[
  {
    "reference": "MEIAPLINNAVAVVTASASAAVSASASVGSSSKDDNVNNLALLASLTLQKVLNINNNNNQHSKNNNHSDNSNSKQHEQQKQQQQPQRRNLDIKSEYDVLNQLLKTGAVFTATPTAPIAIERRKQTQQQQRPYAQQAHQQQQQKQRLNSSISSTHSSTSTNSSYGSSSSEDAAGTSAPTGAGEVVTSAALTPRASTTKASSNKLPEKSKIPSDILDDLASRFIINVPDMELNNLIRMCFQIELAHWFYLDFFCAPESGEDGETPKCVQRKLPSVGIKQFAMQLFQHIPFLNKHFGTVDQILDEWKNYKLSVPTYGAILVSEDHNHCLLVQSYFARNSWGFPKGKINENEDPAHCATREVYEETGFDITDLIDANDYIEAFINYQYTRLYVVRNIPMDTQFAPRTRNEIKCCDWFRIDALPVNKNDAISKAKLGKTSNSFFMIMPFVKRLKKWVNDRKAGIEPRRRKFSGQQSPKAASPTNMNGNCKKDVTGVRNESQRQRHKSMGDLDGVKLNNLNGKGTVGTGAGSAASASATAAINSMNICNSNGKRNKQNAAAGSNQATPTTIATPMTSNGAVVGGTVSSKRQLFHSQSQNDAQQSASNEKQIVSSFDLIRKEKQQQLKAAKAQKQQQQQQQRSRTKSQSDKQYNPQQPVKILGQQQPTTGMDLIAMLSAAAAAQKTPNNEQQTQEKQQQQQRPRPASVSLNLGAGSATGATTSAPDAQYMHRLQRMASGTNLRILKRAQSQQPQQHHQQQLQLEQQQQQQQQLGI",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that can perform manganese ion binding, m7G(5')pppN diphosphatase activity, actin filament binding, RNA binding in pole plasm, P-body to support pole plasm oskar mRNA localization, deadenylation-independent decapping of nuclear-transcribed mRNA, cortical actin cytoskeleton organization, regulation of cytoplasmic mRNA processing body assembly, deadenylation-dependent decapping of nuclear-transcribed mRNA.2. Mn(2+) is required for the proper folding and stability of the protein.3. The protein design should emphasize Polar residues, Basic and acidic residues compositional bias.4. The designed protein must have at least one Nudix hydrolase domain. The designed protein sequence is ",
    "response#1": "AGLEATLPNSYCNAMLQILYFTESLRIKLIEHSCSKEFCLSCELGFLFEMLDKSNASTPCQASNFLRSFRTVPEASALGLILTDRSSNVNLISLIQNWNRFILHQMHYEIFDSSKNSSVLSGSLHAEYPGTPENNQSSDLYDSICDDSAKDDERERSKINAETEISKIFGTKQICINRCIKCHVEKSKDNILLACNMSYPTHIKDTEQYFNFGTILKRSLSTEKNIQAFCENCKKFSPTNQSVKVTALPQMLAINCGLNNQ",
    "response#2": "AGLEATLPNSYCNAMLQILYFTESLRIKLIEHSCSKEFCLSCELGFLFEMLDKSNASTPCQASNFLRSFRTVPEASALGLILTDRSSNVNLISLIQNWNRFILHQMHYEIFDSSKNSSVLSGSLHAEYPGTPENNQSSDLYDSICDDSAKDDERERSKINAETEISKIFGTKQICINRCIKCHVEKSKDNILLACNMSYPTHIKDTEQYFNFGTILKRSLSTEKNIQAFCENCKKFSPTNQSVKVTALPQMLAINCGLNQ",
    "response#3": "AGLEATLPNSYCNAMLQILYFTESLRIKLIEHSCSKEFCLSCELGFLFEMLDKSNASTPCQASNFLRSFRTVPEASALGLILTDRSSNVNLISLIQNWNRFILHQMHYEIFDSSKNSSVLSGSLHAEYPGTPENNQSSDLYDSICDDSAKDDERERSKINAETEISKIFGTKQICINRCIKCHVEKSKDNILLACNMSYPTHIKDTEQYFNFGTILKRSLSTEKNIQAFCENCKKFSPTNQSVKVTALPQMLAINCGLNR"
  },
  {
    "reference": "MNIIKTGIKQSLVSGIYSKVGIRAFCTHYSAELEWVKLSDDNKVATVGLSSFGAQRLGKINYVELPKEHRKCRREEKFGVLESSNATAFGLYAPVSGEVLEVNEKLKKSPSLLNEDPANNWMVKFKVSKPDEFKKLMDSNKYKKFVQWYR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be able to modulate glycine decarboxylation via glycine cleavage system in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "VHDLHGELGATFAEFGGWQMPVAYAGTVGEHQAVRTTVGLFDVSHLGKATVAGPGAAQFVNATLTNDLGRIHPGKAQYTLCCSADGGVIDDLIAYYVADDEIFLVPNAANTAAVVAELAAAAPAGVTVTDQHRDYAVFAVQGPRSAQVLAALGLPTEMEYMAFADAAWDGRPIRVCRTGYTGEHGYEVLPRWADAEPVFRALLEQVRAADGQPAGLGARDTLRTEMGYPLHGHELSREISPVQARTGWISAVP",
    "response#2": "VHDLHGELGATFAEFGGWQMPVAYAGTVGEHQAVRTTVGLFDVSHLGKATVAGPGAAQFVNATLTNDLGRIHPGKAQYTLCCSADGGVIDDLIAYYVADDEIFLVPNAANTAAVVAELAAAAPAGVTVTDQHRDYAVFAVQGPRSAQVLAALGLPTEMEYMAFADAAWDGRPIRVCRTGYTGEHGYEVLPRWADAEPVFRALLEQVRAADGQPAGLGARDTLRTEMGYPLHGHELSREISPVQARTGWVNMTTG",
    "response#3": "VHDLHGELGATFAEFGGWQMPVAYAGTVGEHQAVRTTVGLFDVSHLGKATVAGPGAAQFVNATLTNDLGRIHPGKAQYTLCCSADGGVIDDLIAYYVADDEIFLVPNAANTAAVVAELAAAAPAGVTVTDQHRDYAVFAVQGPRSAQVLAALGLPTEMEYMAFADAAWDGRPIRVCRTGYTGEHGYEVLPRWADAEPVFRALLEQVRAADGQPAGLGARDTLRTEMGYPLHGHELSREISPVQARTGWIG"
  },
  {
    "reference": "MVQSPMISCPLKQTNEIDWIQPLKDYIRQSYGEDPERYSQECATLNRLRQDMRGAGKDSATGRDLLYRYYGQLELLDLRFPVDENHIKISFTWYDAFTHKPTSQYSLAFEKASIIFNISAVLSCHAANQNRADDIGLKTAYHNFQASAGMFTYINENFLHAPSTDLNRETVKTLINITLAQGQEVFLEKQIMDHKKAGFLAKLASQASYLYAQAIEGTQEHAKGIFDKSWVTLLQVKSAHMGSVASYYQALADGESGSHGVAVARLQLAEKHSTSALSWAKSLPSSISPNTNLTSEAGPSLVDIVKFHLANVQSQLATFVKDNDFIYHQPVPSEAGLSAVSKLPAAKAIPVSELYQGQDIQRIIGPDIFQKLVPMSVTETASLYDEEKAKLIRAETEKVETADGEMAASLDYFKLPGSLNILKGGMDQEVMVDEEFQRWCQELAGHDSFAKAFDTLQDRKSEVLATLDQCAKQLDLEESVCEKMRSKYGADWSQQPSSRLNMTLRNDIRTYRDTVHEASASDAQLSATLRQYESDFDEMRSAGETDEADVLFQRAMIKAGSKQGKTKNGVTSPYSATEGSLLDDVYDDGVPSVAEQIARVESILKKLNLVKRERTQVLKDLKEKVRNDDISNVLILNKKSITGQESQLFEAELEKFHPHQMRIVQANHKQTALMKELTKTYGDLLQDKRVRAEQSKYESITRQRNSVMARYKKIYDSFNNLGSGIKQAQTFYAEMTETVDSLKKNVDTFINNRRSEGAQLLGQIEREK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Target a Basic and acidic residues, Polar residues compositional bias in the protein's composition for improved properties.2. The protein must contain a signal peptide for proper functionality.3. The protein contains novel BRO1 domains that confer a unique function or activity.4. The protein design should be able to enhance the efficiency of protein transport. The designed protein sequence is ",
    "response#1": "MPYPFVLPTTSSFSFTSCFSCESHPSLPLNASTRRGVVREDLKAFKRTPREHQVAKLPTVVSSLIAYLPYLFAVDAGLSQQAINGEEIAVILKTAPIIEWRPTLSDNAAVAVPGKDPARVKVHSLEYEVFFVLSCLANAHTLQARTALHPLYVLSTAPVGTQQRQVAVSAATKNLLDAASIYDYLALRAEGLSSGYGMAPPCADISPAALRAQCSLAMAEATLLAVLKDDPYPAVVAQDRNKNDTEWMYKAPDIPKVRAHLFARLCLAASEHAAQAYSLCAGLARAEGGAKVTESFLKC",
    "response#2": "MPYPFVLPTTSSFSFTSCFSCESHPSLPLNASTRRGVVREDLKAFKRTPREHQVAKLPTVVSSLIAYLPYLFAVDAGLSQQAINGEEIAVILKTAPIIEWRPTLSDNAAVAVPGKDPARVKVHSLEYEVFFVLSCLANAHTLQARTALHPLYVLSTAPVGTQQRQVAVSAATKNLLDAASIYDYLALRAEGLSSGYGMAPPCADISPAALRAQCSLAMAEATLLAVLKDDPYPAVVAQDRNKNDTEWMYKAPDIPKVRAHLFARLCLAASEHAAQAYSLCAGLARAEGGAKVTESFLKC",
    "response#3": "MPYPFVLPTTSSFSFTSCFSCESHPSLPLNASTRRGVVREDLKAFKRTPREHQVAKLPTVVSSLIAYLPYLFAVDAGLSQQAINGEEIAVILKTAPIIEWRPTLSDNAAVAVPGKDPARVKVHSLEYEVFFVLSCLANAHTLQARTALHPLYVLSTAPVGTQQRQVAVSAATKNLLDAASIYDYLALRAEGLSSGYGMAPPCADISPAALRAQCSLAMAEATLLAVLKDDPYPAVVAQDRNKNDTEWMYKAPDIPKVRAHLFARLCLAASEHAAQAYSLCAGLARAEGGAKVTESFLKC"
  },
  {
    "reference": "MEDNFLINLLGIDELSLRRILEECDCYSNYRISQLKNWIHRRQVSSFDEMTNLSMKLRDYLKTIAKIEAMPLKTQQFSMDGTRKWLFDAGFDNAVETVFIPDSNRNTLCISSQAGCAVNCSFCFTGQQGFNRNLTTSEIIGQLWWAQKNINNDFFSGNLDKSIINSPKITNIVMMGMGEPLLNYSQVIPALRLMMDENSYALSKRKITLSTSGIVPMIDKLVNDCPVSLAISLHAPNDELRDKLIPINKKYPLQELMKSCRNYVSYAPRDFITFEYCMLDNINDSDKHAFELIELVRTIKCKINLIPFNSFPNSNLKRSTQNRILSFVKILNSQGIVTTVRKTRGDDINAACGQLAGSIKNITKIKTHESIKL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein contains novel Radical SAM core domains that confer a unique function or activity.2. The Proton acceptor of the protein must be engineered to enable effective catalysis of adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.3. The [4Fe-4S] cluster binding site should be located in a region of the protein that is accessible to the ligand.4. The presence of [4Fe-4S] cluster is crucial for the protein to function optimally under specific conditions.5. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs6. A protein that can perform rRNA binding, tRNA binding, tRNA (adenine-C2-)-methyltransferase activity, rRNA (adenine-C2-)-methyltransferase activity in cytoplasm to support rRNA base methylation. The designed protein sequence is ",
    "response#1": "QAGRGTLCVSSQAGCALDCSFCSTGKQGFNSDLTSAEIIGQVWIANKSFGTVPGKIDRAITNVVMMGMGEPLLNFDNVVTAMSIMMEDFGYGISKRKVTLSTSGVVPMIDKLGETTDVSLALSLHAPNDELRNQLVPINKKYPLKMLLDACRRYITRLGKERVLTIEYTLLSNVNDQPEHAEQMIALLKDFPCKINLIPFNPFPHSGYERPSNNSIRRFQDMLHKGGFNVTVRTTRGDDIAAAVARRRARYREGLGEPSYRGGQILEWLWQKRATSIEGMSNLPVRLREHLEESFTLRSLSPAKSQGSRDSTRKFLFRLNDGRYIECVLIPASPALYGERSDRRTLCVSSQVGCAFGCKFCASGLDGFTRNLTADEIAGQVLLAEELSGEKVNNIVFMGMGEPLANLPQLLKALELITSPWGLGIGARHLTVSTSGLVPQIRKLADHPQQIRLAISLHGASNDVRDRIMPVNRKWPVEDLFKALSYWTKRKNQKITLEYILIDGINADPAQARLLAQHAESLRAKVNLIPYNTVDGLKWKRPPEEECHAFRDILAARGIPATLRLEKGHDIDAACGQLRLKEERAEKRRDEP",
    "response#2": "QAGRGTLCVSSQAGCALDCSFCSTGKQGFNSDLTSAEIIGQVWIANKSFGTVPGKIDRAITNVVMMGMGEPLLNFDNVVTAMSIMMEDFGYGISKRKVTLSTSGVVPMIDKLGETTDVSLALSLHAPNDELRNQLVPINKKYPLKMLLDACRRYITRLGKERVLTIEYTLLSNVNDQPEHAEQMIALLKDFPCKINLIPFNPFPHSGYERPSNNSIRRFQDMLHKGGFNVTVRTTRGDDIEAAAAKAEQAFEIEAAEERLLAFGVKNYHAKSFTHFVYQLGVLEPSKMLSLSRDFRKRLQDCVDTRLLPIKKRLVSKDGTVKFLFEVRGPKSLEEIEAVYIPEKSRVTLCVSSQVGCKMGCRFCLTAQLGFRANLSAGDIVRQVMTVNADPELRPVTNIVFMGMGEPFDNLDEVRKATKILTHERGLKLSARKICVSTVGLADRIDSLTKEEPFRLAVSLNSSDDVSRNEVMPINRRWNLERLLESCLRYSQRTNKKVTFEYILMKGVTDRDEDLRNLVRLLSPIPCKLNLIPYNESPFTPFKRPTEERILEFHAGLLKAQFPVFIRKNRGNDIFAACGMLKREHR",
    "response#3": "VGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGSGEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLHSPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKPSLIKENEYDSRVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGSGEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLHSPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALAKLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSESIGGNIDYVVEYRKRRTVCISTQSGCALGCVFCATGQMGFDRHLTVGEIVEQVLWASRKFSTTGYKLTNIVLMGMGEPFHNYVNSMDALNRIEDNTGMAVGARRVTVSTAGHAKAITRFAKEGRRERLAVSLHAATDNLRNKLMPINRRFPLEELMAACRMYSSITHRRISFEWALIDGVNDSQEHAQDLCALINNIDCHVNVIPLNPTEQFNGNNSNEDATMIFKSQLDESGIPCTIRVRRGLSHEQSTGACML"
  },
  {
    "reference": "MSSGSDTPDWPEPAPLYTAQQTRELDRQAIEVHGIPGLTLMQRAGAFAFDCLRQGFPGARRVVVVCGPGNNGGDGHVVARLAHEAGLSVRVLMPVDPSRLRGDAATCAEERRLAGVADEPWRPEALEDADLVVDALLGTGLDRPVKEELAAVIGAINRTGTPVLAIDIPSGLNADTGQPMGCAVRARLTATFIGRKRGLYTGEGPEHAGEIRFDDLGVPGLIYEAVPTRVSLLGLADLLGMLGPRPRTAHKGHFGHVLVVGGAPGYSGAARMAGEAAMRTGAGLVSLATHPAHAAFAGLLRPELMCHAVSDPEGLGPLLERATVVAVGPGLGQGEWGGALLARLLEWDGPLVADADALNLLAAEPCRREDWVLTPHPGEAARLLGCPTAAVQADRFAAADELGERYGGTIVLKGAGTLVREPAGGTGVCPLGNPGMASGGMGDVLTGVIAGLIAQGLAPPAAAALGVCLHAAAADLAAREGERGLLAGDVVARLRTLVNHSC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The presence of Mg(2+) is necessary for the protein to function in the desired environment.2. The AMP, (6S)-NADPHX binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein should have ATP binding, NADPHX epimerase activity, metal ion binding, ADP-dependent NAD(P)H-hydrate dehydratase activity to facilitate nicotinamide nucleotide metabolic process.4. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration5. The protein contains novel YjeF N-terminal domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MWALEINSEALGVPTRLLMENAGRSVKDAVIEALGTVSGKEVVVFVGHGGKGGDGLVSARHLAGEGARVSVFLLGEIKHADALANLDVINEMDYSMKLIEVKDPSQLTPLNADVLIDAMLGTGVKGKVREPFRTAIEVFNKSKGFKVSIDVPSGIDPDTGEELGVSVQPDLVVTFHDLKPGLINKPFKAKVAKIGIPPEAECLAKEDYQHKYSHGHALILSGGAGRTGAARLAARGALRIGAGLVTLGVPGSAQMEVACQITAMMLKRVEDGDGVASALEDGRINALCLGPGLGLDERATGLVAAALRAPAPEPGPSVSRGPGPSPGRAVLLDADALSLLARDPDLFALLHDQCVLTPHAGEFKRLFPDIAEKLNAAATKGPAYSKVDATRAAAKRAGCVVLFKGAETVIADPSGACTINLAKYDRAAPWLATAGSGDVLAGFIAGLLARGYCPMDAAASAAWLHVECARSFGPGLIAEDLPEVLPQVLSYTAASIKRLPARAADSFKNQFGHILIVGGDSGFGGAALMAAQSSARAGAGLISLATRPEHLSAALTRCPEVMAHPVISGQELEPLIEGPSVIAVGPGLGRSPWSEQLLARAGRAKVPLVVDADALNILAEGRVLAGMKRDDWVLTPHVGEAARLLGIDTAEVMANPRAAALAVQQKFSGVVILKGAGTVIAHESGVDLINAGNPGMASGGMGDLLTGIVAALIGQHLPLVEAARLAVWLHGEAGNRAAEEGQRGLLATDLLPWIRRLVA",
    "response#2": "MAAIDRETISMGEPGIDLMERAGQAIAEVVLERLDDADEGCGHDPGCGHDHGDDAGRGVLVLVGKGNNGGDGLAAARLLAAAQVPVTVLLLAPVAELSPDARRNHDRLPQAVTLLISSRRDWLTALTEAADGADLLVDAIFGTGIKPPLREEWSDLLRAINDLGLPCVAADVPSGVDGNDGRVDPVAVAADVTVTMGLPKLGLLLPAGREFAGQVLVADIGFPDEVCTPDFWLYSFPKTAVDTHKYKRGHVSVFSGGPSATGAARLSAMAAARAGAGAVTVLSPENAMQVNAAHLTSIMLREAGSMEEVQEFLSARHPEVLVFGPGLGPKPKVGDFALQLIATVADLNRTSTSSFGIQRPAIVLDADAITSLAHQPQALFDAALLPLGPVLVLTPHEGEFARLFPDIAKDKKQSKLAKARAAAERANAIMVYKGADTVIAAPDGRAVINANGAPWLATAGSGDVLAGIVAALLAQGMQAFEAACAAVWIHAEAGSRFGPGLIAEDLPLALVPVLRELVDARQKVAA",
    "response#3": "MYQADRLAMAAGVPGERLMEAAGWAVAREVRRRFPKGRVAILCGPGNNGGDGFVAARLLERDGWAVRLALLGRPEALKGDAAVMASRWRGAVETLSPGCLDRCDVVVDALFGAGLARGLDGIAKAVIEETLRRRLPVIAVDVPSGVHGDSGQVLGLAPDCVATITFFRKKPGHCLVPGRAKCGEVVVADIGIPASVLLIVGGDSGFGGAALMAAQSSARAGAGLISLATRPEHLSAALTRCPEVMAHPVISGQELEPLIEGPSVIAVGPGLGRSPWSEQLLARAGRAKVPLVVDADALNILAEGRVLAGMKRDDWVLTPHVGEAARLLGIDTAEVMANPRAAALAVQQKFSGVVILKGAGTVIAHESGVDLINAGNPGMASGGMGDLLTGIVAALIGQHLPLVEAARLAVWLHGEAGNRAAEEGQRGLLATDLLD"
  },
  {
    "reference": "MPIKLIQVQRKLNVGINTVVEFLHRNGFSVEDNPNTRISDEQYALLVKEFGKDLPEKDRNFAADRMRKEVPSVKEEKTAEIKTVIPEEFRPKIVMKGHIDLDGGQHKKQQEEPKAKEEPKVKEEPKVKEEPKVKEAPAAPAAQAPVKPAQPAQAPTEKKEEKVIVVEVEKEKTVEKQPVVAEPKVESVKPEQEVEKTEEKDDNLFRLNSVKLESKIKVTGKIDLDALNQSTRPKKKTKEEKRKERDEKQKFNNNRPGNNSNGPGAPHKSPNSPTLIKPNAPAKPGEEGDAKKKRKRIKKDRVDVNNTPGTNYPRPNRDDRPNNDRKPRLKKPVKAEVSEEDVQKQIKETLARLTNKNNKNNKGAKYRRDKRDAAVKREHELMEQEELESKVLKLTEFVTANDLANMMDVSVTEVIGTCMSIGLMVSINQRLDAETINIVAEEFGYKTEYVSADVVEAINADEEDDNEEDWVARPPIVTVMGHVDHGKTSLLDNIRSANVIAGEAGGITQHIGAYNVKLQNGRRITFLDTPGHEAFTAMRARGAKVTDIAIIIVAADDNVMPQTIEAINHASAAGVPIVFAINKIDKPHANPEKIKEELANMNYLVEDWGGKYQSQEISAKKGIGVEELLEKVLLEADLLDLKANPKKRAVGSIIESSLDKGRGYVSTILVENGTLKMGDIVLAGTHQGRIKAMFNERNQRVEKAGPSEPVLILGLNGAPQAGDTFNVLETEQEAREIANRREQLQRELGLRTQKMLTLDDIGRRIAVG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein's functionality will benefit from Basic and acidic residues, Polar residues compositional bias in its structure.2. The designed protein should contain one or more tr-type G domains that are essential for its function.3. The GTP binding site should be located in a region of the protein that is accessible to the ligand.4. The protein can be targeted to cytoplasm and perform GTPase activity, GTP binding.5. For general function, the protein need meet that Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits; One of the essential components for the initiation of protein synthesis; Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. The designed protein sequence is ",
    "response#1": "VTVMGHVDHGKTKLLDALRHTDVVKGEAGGITQAIGAYQVQTEVDDAERAITFIDTPGHEAFTAMRARGAQSTDIAVLVVAADDGVMPQTVEALNHAKAADVPIVVAVNKIDKPEADPDKVRGQLTEYGLVPEEYGGDTMFVNVSARTHEGLDDLLEAIVPRSPVVTVMGHVDHGKTSLLDALRHANVVSGEAGGITQHIGAYQVTAPDSGKKITFIDTPGHAAFTAMRARGAKVTDIVVLVVAADDGVMPQTIEAINHAKAAKVPMIVAINKIDKPDARPDRVRTELLQHEVQVESLGGEVVDVEVSAKNKTNLDRLLEMIALQADILPPVVTVMGHVDHGKTKLLDAIRSTDVVGGEAGGITQHIGAYQVRAKVDGDERPITFIDTPGHETFTAMRARGAQVTDIVVLVVAADDGVKPQTIEALNHAQAAGVPVVVAVNKVDKEGADPAKVRGQLTEYGLVAEEYGGDTMFVDVSARNRTNIDGLLEPRSPVVTVMGHVDHGKTSLLDALRHANVVSGEAGGITQHIGAYQVTAPDSGKKITFIDTPGHAAFTAMRARGAKVTDIVVLVVAADDGVMPQTIEAINHAKAAKVPMIVAINKIDKPDARPDRVRTELLQHEVQVESLGGEVVDVEVSAKNKTNLDRLLEMIALQADILKRSPIITVMGHVDHGKTSLLDAIRKSKVTSTEAGGITQHIGAYTVELNGEAITFLDTPGHAAFTAMRARGAQVTDIVILVVAADDGIMPQTQEAISHCKAANVPLIVAINKIDRPGANIDKVKQELTEYGLVAEDWGGDTICVPVSAHTKEGIDDLLEMILLSSEILPRSPVVTVMGHVDHGKTSLLDALRHANVVSGEAGGITQHIGAYQVTAPDSGKKITFIDTPGHAAFTAMRARGAKVTDIVVLVVAADDGVMPQTIEAINHAKAAKVPMIVAINKIDKPDARPDRVRTELLQHEVQVESLGGEVVDVEVSAKNKTNLDRLLEMIALQADIL",
    "response#2": "PPVVTIMGHVDHGKTSLLDRIRRSKVAAGEAGGITQHIGAYHVETDKGIITFLDTPGHAAFTAMRSRGAKATDIVVLVVAADDGVMPQTAEAIDHARAAGTPIIVAINKMDKESADPDRVLNELTTKQIVPEQWGGDVPVAMVSAHSGQGIDELLDLYLNDLKPVVTVMGHVDHGKTSLLDALRHANVVSGEAGGITQHIGAYQVTAPDSGKKITFIDTPGHAAFTAMRARGAKVTDIVVLVVAADDGVMPQTIEAINHAKAAKVPMIVAINKIDKPDARPDRVRTELLQHEVQVESLGGEVVDVEVSAKNKTNLDRLLEMPRSPVVTVMGHVDHGKTSLLDALRHANVVSGEAGGITQHIGAYQVTAPDSGKKITFIDTPGHAAFTAMRARGAKVTDIVVLVVAADDGVMPQTIEAINHAKAAKVPMIVAINKIDKPDARPDRVRTELLQHEVQVESLGGEVVDVEVSAKNKTNLDRLLEMIALQADILEARDNRKAKGTVVDAKLDKGRGPVASLIVQNGTLNSGDSIIVGTTYGRIRAMFDDKGRKINSAGPSIPAEILGLSEVPSAGDRFYVVKDEKTAREMAEKRKKLARQHSAKLENIFETMGQDEKKTLNIVLKADVRGSLEALQGALSELGNEEVQVRVVGGGVGGITESDANLALASNAVLFGFNVRADAGARKIVEQEGLDLRYYNVIYDIIEDVKKSLG",
    "response#3": "VVVMGHVDHGKTTLLDRIRKTNVAEREKGGITQHIGASQVELPDGRKITFLDTPGHEAFTTLRARGAKVTDISVLVVAADDGVMPQTIEAINHAKAFNVPIIVAVNKIDKPNADPMKVRRELSEHGLIPEEWGGDTIFVDISAKTGQNVDQLLEMKRSPIITVMGHVDHGKTSLLDAIRKSKVTSTEAGGITQHIGAYTVELNGEAITFLDTPGHAAFTAMRARGAQVTDIVILVVAADDGIMPQTQEAISHCKAANVPLIVAINKIDRPGANIDKVKQELTEYGLVAEDWGGDTICVPVSAHTKEGIDDLLEMILLSSEILTRPPVVTIMGHVDHGKTSLLDYIRNSNVVAGESGGITQHVAAYEVTASNGRKITFLDTPGHEAFTAMRARGAQVTDIVILVVAADDSVMPQTIEAINHAKAAGVPIVVAINKMDKPEANPEKIKTQLSEAGVLVEEWGGESQCQEISAKKGLGIEELMEKVLTEAEMRELKAKGTVVDAKLDKGRGPVASLIVQNGTLNSGDSIIVGTTYGRIRAMFDDKGRKINSAGPSIPAEILGLSEVPSAGDRFYVVKDEKTAREMAEKRKEG"
  },
  {
    "reference": "MSILVVTGTGTGIGKTVVTAALACHARLAGLDVAVCKPIQTGTRDGDDDLAVVARLAGVEDLHSLAKFPEPLAPLAAAHRAGADLPSRAALDDLIAAVDRPGRLTLVEGAGGLLVEIGRGGVTLRDLAEDLRAPVLTVVAPGLGTLNHTVLTLEALAHRDVRSEGLVIGSWPAQPGVAEIDNRDALARLGPVRAALPAGAGTLSGTDFERLSVQAFDEAWVRGLVG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must have a active site that is structurally stable and resistant to denaturation.2. A protein should be able to manipulate the activity of enzymes in the biotin from 7,8- diaminononanoate: step 1/2 pathway, either by activating or inhibiting their function.3. The substrate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "TPHPTAQYWRKCQVEELFDLPFMDLVFRAAEIHRQHFDPNAIQLSTLLSIKTGGCAEDCEYCPQSARYDTGVEKQSMLDVNYIVEKAQIAKARGASRFCMGAAWRGPKPKDIETVSAIIKAVKSLGLETCGTFGMLEEGMAEDLKEAGLDYYNHNLDTDPERYHQVIHTRSHDDRMDTLGKVRSAGLKICCGGIVGMNETRAERAGLIASLANLDPQPESVPINQLVKVAGTPLENAEDLDWTEFVRTIAVARITMPKSYVRLSAGRSNMPEAMQALCFMAGANSIFYGDKLLTTENPEEDGDRVLMEKLNLYPLNK",
    "response#2": "TPHPTAQYWRKCQVEELFDLPFMDLVFRAAEIHRQHFDPNAIQLSTLLSIKTGGCAEDCEYCPQSARYDTGVEKQSMLDVNYIVEKAQIAKARGASRFCMGAAWRGPKPKDIETVSAIIKAVKSLGLETCGTFGMLEEGMAEDLKEAGLDYYNHNLDTDPERYHQVIHTRSHDDRMDTLGKVRSAGLKICCGGIVGMNETRAERAGLIASLANLDPQPESVPINQLVKVAGTPLENAEDLDWTEFVRTIAVARITMPKSYVRLSAGRSNMPEAMQALCFMAGANSIFYGDKLLTTENPEEDGDRVLMEKLNLYPLNH",
    "response#3": "TPHPTAQYWRKCQVEELFDLPFMDLVFRAAEIHRQHFDPNAIQLSTLLSIKTGGCAEDCEYCPQSARYDTGVEKQSMLDVNYIVEKAQIAKARGASRFCMGAAWRGPKPKDIETVSAIIKAVKSLGLETCGTFGMLEEGMAEDLKEAGLDYYNHNLDTDPERYHQVIHTRSHDDRMDTLGKVRSAGLKICCGGIVGMNETRAERAGLIASLANLDPQPESVPINQLVKVAGTPLENAEDLDWTEFVRTIAVARITMPKSYVRLSAGRSNMPEAMQALCFMAGANSIFYGDKLLTTENPEEDGDRVLMEKLNLYPLH"
  },
  {
    "reference": "MHGLLLAATLLSLPFNAVAHVPPTTGLVRRGVDLDSFRLNERSQFKISEQVEKDSGVSALHSRNYVDVATRLVKRVAPKATFRLVDDHYIGDTGVGHVYFRQTINGVDVDNGDFNVNIGRNGRVFSYGNSFHTGDVPESNLRVKEDPSNPVQALHGALKALRIPIKAERAVAEATNEEDDEDFVLKGTSGALADPTAKLVYLMKNDGSLALTWRVETDIGDNWLLTYVDAEDTTKVHNVVDYVAHATYKVYRWGIADPTEGELEVISDPWNLRTSEFTWHGNGTTRFSTTIGNNGIAQSNPTGGSEYLNNYRPQPADLKFEYDYSPSMNPPSTYIDASITQLWYSANTYHDLLYMLGFNERSGNFETNNNNQGGKGNDYVILNAQDGSGTNNANFATPPDGRPGRMRMYIWTRANPPRDVCFEEGTVVHEYTHGLSNRLTGGPANSRCLNALESGGMGEGWSDFFATAVRLKPKDTRHTDYPKGAWVANNPRGVRQYVYSTNMTTNPLVYTTVNSLNQVHAIGTVWATILYEVLWNLIDKHGKNDGPTPKFRNGVPTDGKYLAMKLVLDGLALQPCNPNFVQARDAILDADKILTGGRNQCELWKGFAKRELGTGAKWDPRNRVGSTRVPVICRIFT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be optimized for metalloendopeptidase activity. The designed protein sequence is ",
    "response#1": "LLKRSNEISALAHLAVMKAAKLAKNERELEAVFRSTCLSHGSRGQSYGPIVAAGVNGATLHYQTNDMDLEDLVTGERPSLLVDAGGEYRLYCSDITRAYPLSGKFSVEARQIYDIVLDMQTQCMDMIKPGVAWDDIHARAHKVAISGLLRLGILRGSEEELFEKRISVAFFPHGLGHYMGMDTHDVGGNPNHADPNPMFRYLRLRGTLSPSEVVTVEPGVYFCRFIIEPYLSSPELGKYIDSAVLDKYWKVGGVRIEDNLVITE",
    "response#2": "SGEMSVVMDSSLAGVFVHEAFGHAVEADHVLQGATVLKGRLNEKVADESVTIIDDPTLPEFGFFPFDDEGVRAEKKVIVEDGVLKSFLHSRETAKKLGGVAGNARSQGVDVPIVRMSNTYIDTHHYSFDELLEECRDGVYLVGSRGGETNPATGYFHFNAQYGYLIKNGELAEMVRDVSLSGNTLEILRNVKIGREIEFDPGFCGKAGQLVPVSDGSPPVLCR",
    "response#3": "LLKRSNEISALAHLAVMKAAKLAKNERELEAVFRSTCLSHGSRGQSYGPIVAAGVNGATLHYQTNDMDLEDLVTGERPSLLVDAGGEYRLYCSDITRAYPLSGKFSVEARQIYDIVLDMQTQCMDMIKPGVAWDDIHARAHKVAISGLLRLGILRGSEEELFEKRISVAFFPHGLGHYMGMDTHDVGGNPNHADPNPMFRYLRLRGTLSPSEVVTVEPGVYFCRFIIEPYLSSPELGKYIDSAVLDKYWKVGGVRIEDNLVITR"
  },
  {
    "reference": "MISSATLALQSPLPLVAENNRLKLFSGSANIQLAQEVARYLSVDLGPMVRKQFADGELYVQIQESIRGCDVYLMQPTCRPVNDHLMELLIMIDACRRASARQITAVIPYYGYSRADRKTAGRESITAKLVANLITQAGASRILSMDLHSAQIQGYFDIPVDHVYGSPVLIDYLAQKELPDLVVVSPDVGGVARARAFAKKLDDAPLAIIDKRRQAHNVAEVMNVVGDVAGKTAVLVDDMIDTGGTITEAARLLREEGARQVYACATHAVFSPPATQRMVDSVFEEVIVTNTIPVPPERHFPQLRVLSVASLLGETIWRVHEDSSVSSMFR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein have Ribose-phosphate pyrophosphokinase N-terminal domains with specific sequence motifs that are crucial for its activity.2. The designed protein should have a high specificity for Mg(2+) binding, with minimal off-target effects.3. A protein with ribose phosphate diphosphokinase activity functions is required to enable 5-phosphoribose 1-diphosphate biosynthetic process.4. The protein should be optimized for activity in Metabolic intermediate biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "MKLITGNSNLALAKSIAEILNTDLIPASVRYFEDHEIFVELEESIRGEDVYIIQSTSYPANDHIMELIIIIDALRRSSARRITAVIPYYGYSRQDRMTSGRTAISAKVVAEMISKIE",
    "response#2": "MKLITGNSNLALAKSIAEILNTDLIPASVRYFEDHEIFVELEESIRGEDVYIIQSTSYPANDHIMELIIIIDALRRSSARRITAVIPYYGYSRQDRMTSGRTAISAKVVAEMISKIS",
    "response#3": "VPVLLEYLSTKQLSDIVVVSPDVGGVARARAFAKKLGDSPLAIIDKRRQAHNVAEVLNVVGDVKNKTAVLVDDMIDTAGTISEAARLLRKEGARQVYACATHAVFSPPAIERLSTGLFEEIIVTNA"
  },
  {
    "reference": "MNRERDSETTIEEDSQPNDEVVPYSDDETEDELEEQEPAVEPEQNRVNREAEENREPVRKECTWQVKANDRSFHEQPQFMNTTFFCLKESKYANNAIKTYKYNAITFLPMNLFEQFKRAANFYFLVLLILQAIPQISTLAWYTTLVPLLLVLGITAIKDLVDDVARHRMDKEINNRTCEVIKDGRFKTVKWKEVQVGDVIRLKKNDFIPADILLLSSSEPNSLCYVETAELDGETNLKFKMSLDVTDRYLQKESALAAFDGFVECEEPNNRLDKFTGTLFWRNSSYPLDSDKILLRGCVIRNTDFCHGLIVFAGADTKIMKNSGKTRFKRTKIDYLMNYMVYTIFVVLILLSAGLAIGHAYWEAQVGNYSWYLYDGQDNSPSYRGFLNFWGYIIVLNTMVPISLYVSVEVIRLGQSYFINWDLQMYYPEKDTPAKARTTTLNEQLGQIHYIFSDKTGTLTQNIMTFKKCCINGQIYGDNRDASQHQHSRMDEVDFSWNTFADGKLLFYDHYLIEQIRSGKEPEVREFFFLLAICHTVMVDRTDGQINYQAASPDEGALVTAARNFGFAFLARTQNTITISEMGIERTYNVLALLDFNSDRKRMSVIVRTPEGHIRLYCKGADTVIYERLHPMNPTKQETQDALDVFANETLRTLCLCYKEIDENEFAEWYKKFVAASLALTNRDQALDKVYEEIEKDLILLGATAIEDKLQDGVPETISKLAKADIKIWVLTGDKKETAENIGFACELLTEETTICYGEDINALLQTR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The designed protein's functionality will benefit from Acidic residues compositional bias in its structure.3. A protein that contributes to Golgi organization, negative regulation of DNA-templated transcription by functioning in stereocilium with ATP hydrolysis activity, ATP binding.4. The designed protein must have at least one P-type ATPase C-terminal, P-type ATPase N-terminal domain.5. We need a protein that can perform ATP + H2O + phospholipidSide 1 = ADP + phosphate +   phospholipidSide 2. with high efficiency. The designed protein sequence is ",
    "response#1": "GIKVIDLVCPFAKGGKVGLFGGAGVGKTVNMMELINNIAKAHSGLSVFAGVGERTREGNDFYHEMADSGVVNLEKLEDSKVAMVYGQMNEPPGNRLRVALTGLTIAESFRDEGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGRLQERITSTKVGSITSIQAVYVPADDLTDPSPATTFAHLDSTVVLSRDIASLGIYPAVDPLDSTDRY",
    "response#2": "GIKVIDLLAPYAKGGKIGLFGGAGVGKTVLIMELIRNIATEHGGYSVFTGVGERSREGNDLWNEMNESGVIEKTALVFGQMNEPPGSRMRVGLTGLTMAEYFRDELGQDVLLFIDNIFRFIQAGSEVSALLGRIPSAVGYQPTLATEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFTHLDATTVLSRHIVEQGIYPAVDPLDSTNR",
    "response#3": "GIKVIDLVCPFAKGGKVGLFGGAGVGKTVNMMELINNIAKAHSGLSVFAGVGERTREGNDFYHEMADSGVVNLEKLEDSKVAMVYGQMNEPPGNRLRVALTGLTIAESFRDEGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGRLQERITSTKVGSITSIQAVYVPADDLTDPSPATTFAHLDSTVVLSRDIASLGIYPAVDPLDSTDRY"
  },
  {
    "reference": "MLEWRDEGALLSVRRHGESAAIIEVFTASHGRHLGVVRGGASRRHAPVLQPGAQLDLTWKARLDDHMGAFTVEPLRARTGIFGDRLALAGLNAICAMLHVVLPEREPHVGLWRESIELMDALAAPGWPAAYLRWEMRLLEEAGFGLDLTRCAVTGSREDLAFVSPRTGRAVGRAAAGDWADRLFPLPLALLGQGPASGPEVRQGLAITGHFLARELPLAGRPLPEARARLIDLLARG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Involved in DNA repair and RecF pathway recombination.2. The protein design must be focused on enhancing the efficiency of DNA repair. The designed protein sequence is ",
    "response#1": "MQIKTKAIVISAIKYQEKSLIVKCFTLSDGLKSYFVRDAFSSKKSNQKIAYFQPLTILEIEAVHKNKGTLERFKEVKIATPL",
    "response#2": "MITSLLAVVESVADLYALERSGEFRGLYHVLGGALNPLEGIGPKELNLEGLFRRLEGVEEVVLATSMTVEGEATALYLAEELKKRGVRVTRPAYGLI",
    "response#3": "MSSLLAVVESVADLYALERSGEFRGLYHVLGGALNPLEGIGPKELNLEGLFRRLEGVEEVVLATSMTVEGEATALYLAEELKKRGVRVTRPAYGLPVGGSLEYADEVTLGRQ"
  },
  {
    "reference": "MSEIDVVILAAGKGSRMKDNFPKPLHEVAGLPILEWVCRAVRKIEPHKIIAVQSASEDFASYVDETVVQAQQLGSADALQAAFSKVDAEKILVINGDMPLVTGDVLADLVAKGEGFDAAVLTTILKHPFGYGRIIPVGQRNVVEQIVEEKDATDDQRQIQLINSGIYLFRTEYIKRTISQVTANNAQKEFYLTDALPGARIVQADDWRDLLGVNDQKQLADANQLARKRINEQLMANGVSMIDPQSTYIDANVVVGAGTVIKPGTVIERDSVIGADNEIGPYAHLREKTVTGTDVHIGNFVETKNAEIGAHTHIGHLTYVGDARIGESVNIGAGTIFVNYDGKNKHITKVGDHAFIGSNTKLIAPVDIAREAITAAGSTITADVAEHAMGIARQRQTNKPEFWDRLPHEDFAQAYREKKRQENQAPDES",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.2. The designed protein must be able to regulate peptidoglycan biosynthetic process, cell wall organization.3. The designed protein must have at least one MobA-like NTP transferase domain.4. The designed protein should be able to compete with endogenous proteins or inhibitors for UDP-N-acetyl-alpha-D-glucosamine binding.5. The protein design should take into account the physiological conditions of the Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MAAGLGTRMHSQVPKHLHPLLGRRMVDWVLAAARELGVEPLVVIAAPATASEFVGIEVTVQEAPLGTGDAVRSARSALEDRAENVLVLSGDTPLLTSALLGDLLETHRRENAWATVLSFSVHDATGLGRIVRDATGEVLGIVEQKDASPEQLAVCEINSGIYAFDLAALRTALGRLSSNNAQGELYLTDVLAIARADGGKVVAVQADDPWLTEGVNDRVQLAALGAELNRRVVEGWMRAGVTIVDPQSVTIDDSVLLHANVVIEPQTHLRGKTTIQAGSRIGPGSLVENSTIGENVTVLFSVVSDSVVRPETRVGPYAHIRGQAEVGAGCRIGNFVELKNAQLGEKTNVAHLSYVGDATLGDRVNIGAGTITVNYDGVNKHRTTIGDRSKIGSNNTLVAPLTIGEDVTTAAGSTITEDVPNDCLAIARSRGVRRIEDWVVAGRPPGSAAAQASAESADQES",
    "response#2": "MAAGLGTRMHSQVPKHLHPLLGRRMVDWVLAAARELGVEPLVVIAAPATASEFVGIEVTVQEAPLGTGDAVRSARSALEDRAENVLVLSGDTPLLTSALLGDLLETHRRENAWATVLSFEAADPTGYGRIIREGNRIVAIVEEKDATDAQKRIKEIYSGIMAVPTSRLKQWIARLDNKNAQKEFYLTDLIGFCVQEGVPVISAQPEDPLEVMGVNSPLQLAELERIFQQRVAEKLLETGVTIVDPQSVTIDDSVLLHANVVIEPQTHLRGKTTIQAGSRIGPGSLVENSTIGENVTVLFSVVSDSVVRPETRVGPYAHIRGQAEVGAGCRIGNFVELKNAQLGEKTNVAHLSYVGDATLGDRVNIGAGTITVNYDGVNKHRTTIGDRSKIGSNNTLVAPLTIGEDVTTAAGSTITEDVPNDCLAIARSRPPQKIEK",
    "response#3": "MAAGLGTRMHSQVPKHLHPLLGRRMVDWVLAAARELGVEPLVVIAAPATASEFVGIEVTVQEAPLGTGDAVRSARSALEDRAENVLVLSGDTPLLTSALLGDLLETHRRENAWATVLSFEVPDPGRYGRLVTEGTALTKIVEYKDATEAERAITLCNSGVIAADCALLFDLVAEVGNDNAAGEYYLTDIVALARARGLSAGVVTCAESETLGINTRSEGVRLADPARLDVRGTLSCGRDVFIDVNCVFEGQIELGEAVEIGPNCVLKNARIGAGTRLAAFSHVEDAVVGPDAVIGPFARLRPGTELAAGVHIGNFVEIKNSKIAANSKANHLAYVGDAIVGSRVNIGAGTITCNYDGANKFQTVIEDDVFIGSDTQLVAPVTVGRGATLGAGTTLTKDAPPDTLTVSRAKQDVKPGWADRFRALKRALKK"
  },
  {
    "reference": "MNCTRVLQILNPRPMPSPIMPVDVAMRICLAHSPPLRSFLSSYEDCKSRNLVNQYKPLRSCISSKTEDDTANITWKSPETKAKKKVVFADSKGMSLTAVHVFKEFEEDLMLDLQFELSDLEDAIVGLKAEKEKNFSLGFPQPAADYLDFRNRLKKNLVCLENCIIQERSVTGTVKVSNVSFEKVVHVRITFDSWKSHTDIPCTYMNNVYGCEDVDTFSFSIDLPSFVAPHEQVEFCLSYKTHDMTYWDNNDGKNYKLVHTENDSSQASVIQNATTDFKAQGKRPDMEFDQFGSPRTSSGFFPEWQSWGHIENNTPYW",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must have at least one CBM21 domain.2. The protein must be able to protein phosphatase 1 binding, glycogen binding. The designed protein sequence is ",
    "response#1": "TALPTKNNVRFRLLATTQVGEDVFLVGSIPELGSWDVKKAVPLNADIYADNCHQWYVDIELPTAVAFEYKFIRKRGGEVVWEQDPNRKYTVPQTCGVSGAIKRDTWN",
    "response#2": "TALPTKNNVRFRLLATTQVGEDVFLVGSIPELGSWDVKKAVPLNADIYADNCHQWYVDIELPTAVAFEYKFIRKRGGEVVWEQDPNRKYTVPQTCGVSGAIKRDTWS",
    "response#3": "TALPTKNNVRFRLLATTQVGEDVFLVGSIPELGSWDVKKAVPLNADIYADNCHQWYVDIELPTAVAFEYKFIRKRGGEVVWEQDPNRKYTVPQTCGVSGAIKRDTWR"
  },
  {
    "reference": "MDNKNNYNNPDNNQPKMPRFNMNWFYAVVLLGLVLLFLSGGGEALTGSPAAKEASYTTFKAYIEKGYAERVVVNKDDGTLKMYVRATKIKDVFHKTAKEVGPYPYVSVQTGSIDELERYLTEAQKQGKIVEFSYENQKSNLLFDILINTLPWILLIVFWFWLMRRMSGGGAGGGGGVFSVGKSKARLYEKGNDLGITFKDVAGQEGAKQEVQEIVEFLKNPQKYTELGGKIPKGALLVGPPGTGKTLLAKAVAGEAGVPFFSMSGSDFVEMFVGVGASRVRDVFSQAKQKAPCIIFIDEIDAVGRARSKNPAMGGNDERENTLNALLTEMDGFGTNSGVIVLAATNRVDMLDKALLRAGRFDRQIHVDLPDLAERKAIFQVHLRSVKIDNTVDIDFLARQTPGFSGADIANVCNEAALIAARHNSSHVGKQDFLDAVDRIIGGLEKKTKVMTAEEKRSIAIHEAGHATISWFCQHADPLVKVTIVPRGQALGAAWYLPEERVITTKEEMLDEICALLGGRAAEDLFTGHISTGAMNDLERATKSAYGMVAYAGMSDKLPNICYYNNQEYQFQRPYSETTAKIMDDEVLKMINEQYARAKQILTEHKDGHKQLADLLFQKEVIFAEDVEHIFGKRPWESRSQIIIKENAADDDVPKLEDMPDEVKQAQAEHEKAVAEARNAETQAPADTNDTAQS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be involved in protein catabolic process, cell division, proteolysis by localizing to plasma membrane and exhibiting zinc ion binding, ATP hydrolysis activity, ATP binding, metalloendopeptidase activity, ATP-dependent peptidase activity.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.4. The designed protein have AAA+ ATPase domains with specific sequence motifs that are crucial for its activity.5. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.6. The protein must exhibit the following characteristics: Plays a role in the quality control of integral membrane proteins.; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins.7. The protein should exhibit Basic and acidic residues compositional bias.8. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+), ATP binding. The designed protein sequence is ",
    "response#1": "MLMVGPPGTGKTLLAKAVAGEADVPFFSISGSDFVEMFVGTGASRVRDMFKKAKQAAPCIVFIDEIDAVGRQRGAGMGGGNDEREQTLNQLLVEMDGMEENIGIVIIAATNRPDVLDPALLRSGRFDRQITVHLDVDLKEIAKQTPGFVGADLANLLNEAALLAARRNKTEIDASDVDKVNQIVEEYTKAPPELKEKFSYDDVSFEVIAKRTPGFTPADLENMINEAALLAARKNMKTVTMELIEKARIISDAEKLIIAYHEGGHAVVQRVLPKCDPVSKVTIVSRGMALGYTMALPTEDRYLQSKSEFEDKLAGLLGGNVAERLVFGDTTTGSSNDIEKATGLARRMVTEFGMSDRLGPLSFGKRDELVFLGREIGEQRNYSDEIARQIDEEVRALVDRAYERATEVLETHRDRLEGLAQKLIAEETVDADAFEKLSKKIGE",
    "response#2": "MLMVGPPGTGKTLLAKAVAGEADVPFFSISGSDFVEMFVGTGASRVRDMFKKAKQAAPCIVFIDEIDAVGRQRGAGMGGGNDEREQTLNQLLVEMDGMEENIGIVIIAATNRPDVLDPALLRSGRFDRQITVLSNNIKKYEMIENIFKKSTDNFKLIETEVNWHNIASRTPGFSGAQLANVLNEAAILMVRENKTEIGLVEINKSNIFEEIFEKTFKSKDKIIINKINL",
    "response#3": "MLMVGPPGTGKTLLAKAVAGEADVPFFSISGSDFVEMFVGTGASRVRDMFKKAKQAAPCIVFIDEIDAVGRQRGAGMGGGNDEREQTLNQLLVEMDGMEENIGIVIIAATNRPDVLDPALLRSGRFDRQITVLFPDTDRATDLIETRLFSTDSDTRERMLRDVAKYAQSVNWHNIASRTPGFSGAQLANVLNEAAILMVRENKTEIGLVELLGRGYAGTDKAIGIAELIRTITSPQLGASIDEALDIFTDEAEQWDSFEEAFRAHTQ"
  },
  {
    "reference": "MEPVDPKIEPWNQPGSRPKTACTKCYCKKCCYHCPVCFLNKGLGISYGRKKRRQRRQAPPGSKNHQDPVSKQPLSQTQREQTGPEKSKKEVESKAEPDRFD",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Incorporating a Nuclear localization signal, RNA-binding (TAR), and protein transduction motif is critical for the successful design of the protein.2. The ideal protein would be one that plays a critical role in modulation by virus of host chromatin organization, positive regulation of viral transcription, suppression by virus of host type I interferon-mediated signaling pathway, negative regulation of peptidyl-threonine phosphorylation, suppression by virus of host translation initiation through cyclin binding, protein serine/threonine phosphatase inhibitor activity, actinin binding, trans-activation response element binding within host cell cytoplasm, host cell nucleolus.3. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MEPVDPSLEPWKHPGSQPRTACTPCYCKKCCFHCQVCFITKGLGISYGRKKRRQRRRAPQDSQTHQASLSKQSAPQPRGDPTGPKESKKKVERETETDPDH",
    "response#2": "MFILYYDD",
    "response#3": "MEPIDPSLEPWNHPGSQPTTACSKCYCKKCCWHCQLCFLKKGLGISYGRKKRKHRRGTPQSHKDHQTPIPKQPISRTQGNPTGPKESKKEMXSKAEADRFD"
  },
  {
    "reference": "MVLVTLLGLSWFCSPLAALVLDFNNIKSSADVQGAGKGSLCASDRDCSEGKFCLAFHDERSFCATCRRVRRRCQRSAVCCPGTVCVNDVCTAVEDTRPVMDRNTDGQDGAYAEGTTKWPAEENRPQGKPSTKKSQSSKGQEGESCLRTSDCGPGLCCARHFWTKICKPVLREGQVCSRRGHKDTAQAPEIFQRCDCGPGLTCRSQVTSNRQHSRLRVCQRI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.2. A protein with co-receptor binding, receptor antagonist activity is needed to enhance Wnt signaling pathway, negative regulation of canonical Wnt signaling pathway in a specific context.3. Ensure the presence of a signal peptide within the protein.4. The protein must exhibit the following characteristics: In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease .; Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. The designed protein sequence is ",
    "response#1": "RCQPIEIPMCKDIGYNMTRMPNLMGHENQREAAIQLHEFAPLVEYGCHGHLKFFLCSLYAPMCTEQVSTPIPACRVMCEQARLKCSPIMEQFNFKWPDSLDCSKLPNKNDPNYLCMEAPC",
    "response#2": "ARIWIGIWSVLCCASTLFTVLTYLVDMKRFSYPERPIIFLSGCYTMVAIAYIAGFLLEDKVVCNERFAEDGYKTVAQGTKKEGCTFLFMMLYFFSMASSIWWVILSLTWFLAAGMKWGHEAIEANSQYFHLAAWAVPAIKTITILAVGQVDGDTLSGVCFVGINNVDALRGFVLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEKLEKLMVRIGIFSVLYTVPATIVIACYFYEQAFREQWEKSWISQSCKTYAIPCPSTGHPPMSPDFTVFMIKYLMTLIVGITSGFWIWSGKTLNRRRAASLGEMLREVEALMEDTQHKLRNAVQEMEAEEEGAKKLSEVNFENLPPTYHNESNTETRIGNKTVQTHQEIDKVTDNRTGSTIFSETIITSIKGGENKRNHECIIDEDCETGKYCQFSTFEYKCQPCKTQHTHCSRDVECCGDQLCVWGECS",
    "response#3": "KGCELCSEVNGCLKCSPKLFILLERNDIRQVGVCLPSCPPGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCQEALYLHKGRCYPACPEGSTAANSTMEC"
  },
  {
    "reference": "MANILIAVSGSISAYKAADLVSELGKAGHQVQCILTSSAKEFVSPLVLETLSGRKARVEQFGPDVSGTEHIDFARWAEVMVFAPASANMLARLALGLADDLPSTVALATEAPLLIAPAMNTVMWEKPVVQQHLATLVERGATVIEPASGLLACGEVGVGKLAPVATIVAAIETALRPGGVQDLQDERILITAGPTTTAIDAVRYITNHSTGRMGAAMAEEALKRGAVVDYVLGVDKGVVRPKPPRGTEERLHLVEVRTAEEMAAAALERLPQATGVVATAAVMDYAVAAPSASKLKRASDAIALDLVPSTDVLGSLRQAANGQWFLGFAAETDDVEAHGAGKLKAKGLDFLFANEVARLGAAGQTGFAVGTNAGVLLSAKGARHELALGPKSEIARQIWDLIA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be optimized for activity in Cofactor biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The protein contains novel Flavoprotein domains that confer a unique function or activity.3. The designed protein must have a Proton donor that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "MEGLKVLVTAGPTQEALDPVRCLTNHSTGRMGYAVARAAAARGAEVTLVSGPTALKKPAYVETVDVVSAQDMFEAVTARAPEQDIIIKAAAVADYRPAQVAQDKVKKGEGELSIPLERTRDILAWLGEHRGPGQFLCGFSMETRDMVENSRRKLEKKHLDLIAANNLKEPGAGFAVPTNLLTLH",
    "response#2": "VGRIIGSIGVVTTQTTGRHQGILTSWVSQASFTPPGIMLAIPGEFDAYGLAGQNKAFVLNLLQEGRSVRRHFDHQPLPKDGDNPFSRLEHYSTQNGCLILAEALAYLECLVQSWSNIGDHVLVYATVQAGQVLQPNGITAIRHRKSGGQC",
    "response#3": "TSNSYLIQADHTALIDPPGESFCDLYLAELPKYLDLAQLDYIVASHVNPNRMVTLEQLLRRATKAKLICSRPAAKVLKATFPHWEERFQTVRSQDMLDLGRGHKLQLMTLPTPRWPDGLCAYDAGSQILFSDKLFGTHVCGDAIFDEDWRQLGGDRRFYFDCLHAPQTRQVETALDQFDPLTLKMIAPGA"
  },
  {
    "reference": "MANQLSYSSLGWPYQPNASVVDTMPKEMLYMIHEHWYAFPPMNPLWYSILGVAMIILGIICVLGNGMVIYLMMTTKSLRTPTNLLVVNLAFSDFCMMAFMMPTMASNCFAETWILGPFMCEVYGMAGSLFGCASIWSMVMITLDRYNVIVRGMAAAPLTHKKATLLLLFVWIWSGGWTILPFFGWSRYVPEGNLTSCTVDYLTKDWSSASYVIIYGLAVYFLPLITMIYCYFFIVHAVAEHEKQLREQAKKMNVASLRANADQQKQSAECRLAKVAMMTVGLWFMAWTPYLIIAWAGVFSSGTRLTPLATIWGSVFAKANSCYNPIVYGISHPRYKAALYQRFPSLACGSGESGSDVKSEASATMTMEEKPKSPEA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Visual pigments are the light-absorbing molecules that mediate vision; They consist of an apoprotein, opsin, covalently linked to cis-retinal..2. The protein should have a Helical; Name=5, Helical; Name=2, Helical; Name=6, Helical; Name=7, Helical; Name=3, Helical; Name=4 domain that is long enough to span the entire membrane.3. The designed protein should contain Cytoplasmic, Extracellular topological domains.4. A protein that can perform G protein-coupled receptor activity, photoreceptor activity in membrane to support visual perception, phototransduction. The designed protein sequence is ",
    "response#1": "GNGIVIWIFSTSKSLRSASNMFVINLAVFDLMMMLEMPLLIMNSFYQRLVGYQLGCDVYAVLGSLSGIGGAITNAVIAFDRYKTISSPLDGRINTVQAGLLIAFTWFWALPFTILPAFRIWGRFVPEGFLTTCSFDYFTEDQDTEVFVACIFVWSYCIPMALICYFYSQLFGAVRLHERMLQEQAKKMNVKSLASNKEDNSRSVEIRIAKVAFTIFFLFICAWTPYAFVTMTGAFGDRTLLTPIATMIPAVCCKVVSCIDPWVYCLRNAELRNAFKKVLRAAVLACQACCMLAIFLSMLVSYRCRRNKRIWASGVVLLETVLFGFLLLYFPVFILYFKPSVFRCIALRWVRLLGFAIVYGTIILKLYRVLQLFLSRTAQRSALLSSGRLLRRLGLLLLPVLGFLAVWTVGALERGIQHAPLVIRGHTPSGRHFYLCHHDRWDYIMVVAELLLLCWGSFLCYATRAVLSAFHEPRYMGIALHNELLLSAAFHTARFVLVPSLHPDWTLLLFFFHTHSTVTTTLALIFIPSLKSFF",
    "response#2": "ENFTVMLATYKFKQLRQPLNYIIVNLSLADFLVSLTGGTISFLTNARGYFFLGNWACVLEGFAVTYFGIVAMWSLAVLSFERYFVICRPLGNVRLRGKHAALGLLFVWTFSFIWTIPPVFGWCSYTVSKIGTTCEPNWYSNNIWNHTYIITFFVTCFIMPLGMIIYCYGKLLQKLRKVSHDRLGNAKKPERQVSRMVVVMIVAYLVGWTPYAAFSIIVTACPTIYLDPRLAAAPAFFSKTAAVYNPVIYALCNKTFRTTILQMFKM",
    "response#3": "LNAVVLWVTVRYKRLRQPLNYILVNISASGFVSCVLSVFVVFVASARGYFVFGKRVCELEAFVGTHGGLVTGWSLAFLAFERYIVICKPFGNFRFSSRHALLVVVATWLIGVGVGLPPFFGWSRYMPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIVPLSLIIFSYSQLLSALRAVAAQQQESATTQKAEREVSRMVVVMVGSFCLCYVPYAALAMYMVNNRDHGLDLRLVTIPAFFSKSACVYNPIIYTLRNTDVKNAFKKLLCKR"
  },
  {
    "reference": "MPTALDRVQVSHFVAEESFARHLDDLDPCSAFRSRFAVPCGNAAQPNVYFVGNSLGAMPLATRTMLMEELDDWSTLGVEGHFHAKRPWYSWHEQVREPLARLVGAKPSEVVAMNSLTTNLHLMLTTFYRPEGNRTKILIEKGAFPSDIFALRSHVAARGLDPDVHVIEIAARVDEWLLDETDIEAECERLGDTLACAVLPGVQYRTGQAFDLARLTRAVHAVGATCGFDLAHAAGNIDVQLTASMCDFAVWCSYKYLNAGAGAVGGCFVAERHGRDTSLPRLAGWWGNDPTTRFQMSDRFIPVASADAWQLSNPPILSLVPLLASLQIFDEATIPVLRRKSIVMTGYLEYLIRDALRGHAELLTPASASRRGCQLSLLFKDNARARFGALTAAGIVCDYREPGIVRLAPTPLYNTFHEVWRAAQAVKSACVS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be optimized for activity in quinolinate from L-kynurenine: step 2/3 pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The protein should have a high affinity for pyridoxal 5'-phosphate.3. The ideal protein would be one that plays a critical role in quinolinate biosynthetic process, anthranilate metabolic process through 3-hydroxykynureninase activity, kynureninase activity within cytoplasm. The designed protein sequence is ",
    "response#1": "KQELDDWAKLAVDAHLDGATPWYSYHETLRQPTARLVGAQPNEVICMNSLTVNLHLMMATFYRPTKSRNKILMEEPAFPSDTYAIKSQIVHHGFDPKEALILARPRAGEFTVRLHDIEAALKKHGEQIAVVVVGGVNFFTGQLFDIEEITSAARKRGCVVGFDLAHAIG",
    "response#2": "KRGDETAVLVVSAVQYLTGQWFDIPAIVKAARAKKIIVGVDCSHAVGNVPLHLHDWDVDFAYWCTCKYLNSGPGSLSGLFVHNKHTPCTIPLECLNGLWSSDIKSCFSEHRRFEPAPGASPLQISTPSAACHVILAPSLKLMASVGMEAIRQKSLLLTAYLELLVSE",
    "response#3": "KRGDETAVLVVSAVQYLTGQWFDIPAIVKAARAKKIIVGVDCSHAVGNVPLHLHDWDVDFAYWCTCKYLNSGPGSLSGLFVHNKHTPCTIPLECLNGLWSSDIKSCFSEHRRFEPAPGASPLQISTPSAACHVILAPSLKLMASVGMEAIRQKSLLLTAYLELLVSQ"
  },
  {
    "reference": "MTTVNHARPLAIVILAAGKGTRMKSDLHKVLHPIAGRPMIEHLMASAAELAPERQVVVVGAGREQLEAAMGDRAAIAVQEPQHGTGHAVQQAETALAGFAGDVLILYGDVPFVSAGTMRSMIGRLHGEDNPAVVVLGFEPAETLQYGRVIAEGDRVLKMVEHKDANDVERACRLCNSGLMAVRSEDLFGLLARVGNENAQGEYYLVDIVNIANADGRTCAVVVTADADEVAGINSRGELAEAEERWQQRRRAQAMADGASLVAPHTVWFSWDTRLGRDVLVEQNVVFGPGVSVADGARIRAFSHLEGASLASGTEVGPYARLRPGAVLEEKAKVGNFVEVKKAVLGKGAKANHLSYIGDAEVGAGANIGAGTITCNYDGYFKYKTVIGERAFIGSNSALVAPVRIGADAIVAAGSAVTRDVGDGELRIVRAEQLVKQGWADRFHDAMKRKKAEAKAGRK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should take into account the physiological conditions of the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway.2. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).3. The designed protein should have a high specificity for acetyl-CoA binding, with minimal off-target effects.4. A protein that can perform magnesium ion binding, glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity in cytoplasm to support cell morphogenesis, lipid A biosynthetic process. The designed protein sequence is ",
    "response#1": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVSAYLPNPKGYGRIIRDSKGVVQKIVEEKDATSDEKQIQEINTGILCVSAKHLKEWLPKLSNDNAQKEYYLTDIVGFAVNDGLPVVSVQPAFPEDVTGVNNRLQLAELERWYQKLEANRLMTEGVMLMDPARIDVRGELICGADVEIDVNCIFEGTVKLGNRVKVKANCILKDVTVSEGSTIHPFSHVEEAKIGQNCKIGPYARIRPETQLIDNVHIGNFVEVKKSQIASGSKVNHLSYIGDTIIGQNVNIGAGTITCNYDGAYKHQTIIEDNVFVGSDTQLVAPVKVSYGSTIGAGSTITRDTPENELTLSRSKRVEEI",
    "response#2": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVVTTKVADPTGYGRIVRNAAGEVLRIVEQKDASAEELAISEVNTGIMVAPTAALKDWLASLSNDNAQGEYYLTDVIAMAVADGLPVGVAQPADAAEVAGVNNRLQLAELERAYQQRLASELMLAGVTIVDPTSVWVDVGVDLAPDCEILPGVQLHGATSVATGARIGPDTTLTDTRVGAGAEVVRSHVLDSRIEAGARVGPYAYLRQRVVVGPGAKIGTFVEAKNTRLDDGAKIPHLSYMGDATIGAGANVGAGSITANYDGVHKHPTVVGPQARAGSNTMFVAPVELGAGAYTGAGTTLREDVPAGALAVSTPERVVAGWARPAKKK",
    "response#3": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVVTTTLDDPSGYGRIVRDADGAVTRIVEQKDASPEELAIHEINSGILAAPTVLLRQWLAQLDNNNAQGEYYLTDIVAQAVADGVPVVVAQPENADEVMGVNDRVQLAELERGYQQRQAERLMVQGVSLIAPETVWFSSDTQVSSDVIIEPNVIFGPGVSIASGATIYGHSHIEGATIGANAKVGPFARLRPGAVMEEGSKVGNFVEMKKATLGKGAKANHLTYLGDAEVGEGANIGAGTITCNYDGYFKYKTVIGKGAFIGSNSALVAPVNIGDGAIVGAGATLTKDVAAGDLALVRAEKTQIAGWKRPKRVK"
  },
  {
    "reference": "MAAGGGGGGVSRAALARPPIHRGTSAPGGAIAAAGGDGDGDEASRLLGRAQPREAPYLIPRPDGDLAVPDDLQYATLDLTGDPVAVGAGSYGSVLVYGSVAVKTLRAGFGHEAVMTLLAAEEARSAGVRGVVRLMGLSAPLRQLMFPAYEMDMDAYRRSLTARPGHVVHALGRVFTELGRALVFLNGRGLSHLDVKGGNIFVRTCGNMVVTAVIGDFSLMALNSRSALADPRFRLARRKALKITSLARSPPTGVLLGHARDRPTRVLMDFINGRPPPPGPLPYEVGLAIDLCALGHVLLDVALGLRPQRGQALTREYAVEVLARRCVLFAALLPPGSGPSAEALAGDILEEELAAGFREGVASSRPGNQPPRTVAPLLELVARFCGEDGGARFAELAA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Phosphorylation of glycoprotein E (gE) by UL13 alters its subcellular localization, from the host early endosome to the plasma membrane2. The designed protein have Protein kinase domains with specific sequence motifs that are crucial for its activity.3. The Proton acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.4. The protein design must be able to perform protein serine/threonine kinase activity in order to contribute to protein phosphorylation. The designed protein sequence is ",
    "response#1": "MSVNASDQERTPWQVTFKPKSKETCQGSLISDQWVLTAAHCFHDAQMEDRHLWRVIVGDPTSHHGKEFHVEEVLVAPGFNVHAKQSQGISEFYADDIALLKLSQRVKMSSHARPICLPCTVGANMALRRSPGSTCKDHETELLSQQKVPAHFVALNGNRMNINLRTGPERTSCIEAVSQNKGSFPGLTNVSEVVTDQFLCSGMEGDDSPCKGESGGAVFLERRYRFFQVGLVSWGLFDPCHGSSNKNLRRKPPHGVVPRDFHISLFRLQPWINKPS",
    "response#2": "MSVNASDQERTPWQVTFKPKSKETCQGSLISDQWVLTAAHCFHDAQMEDRHLWRVIVGDPTSHHGKEFHVEEVLVAPGFNVHAKQSQGISEFYADDIALLKLSQRVKMSSHARPICLPCTVGANMALRRSPGSTCKDHETELLSQQKVPAHFVALNGNRMNINLRTGPERTSCIEAVSQNKGSFPGLTNVSEVVTDQFLCSGMEGDDSPCKGESGGAVFLERRYRFFQVGLVSWGLFDPCHGSSNKNLRRKPPHGVVPRDFHISLFRLQPWIQEHT",
    "response#3": "GVGNMSVNASDQERTPWQVTFKPKSKETCQGSLISDQWVLTAAHCFHDAQMEDRHLWRVIVGDPTSHHGKEFHVEEVLVAPGFNVHAKQSQGISEFYADDIALLKLSQRVKMSSHARPICLPCTVGANMALRRSPGSTCKDHETELLSQQKVPAHFVALNGNRMNINLRTGPERTSCIEAVSQNKGSFPGLTNVSEVVTDQFLCSGMEGDDSPCKGESGGAVFLERRYRFFQVGLVSWGLFDPCHGSSNKNLRRKPPHGVVPRDFHISLFRLQPWLRQHLR"
  },
  {
    "reference": "MPGFLFARMPWVWVVLILLWTSLAQGMSESQLTELRKETEHMFYHGFDNYMQHAFPEDELRPLSCTPLTREREGSGNADLNDVLGNYTLTLIDSLSTLAILSSGPDESGERAWGYFQNGVRDFVVLYGDGSAGPAGQGEKGRGLDMDSKVQVFETVIRGLGGLLSAHLFAVGDLPISGYRPPTAEAAFAKAWDKSGFGTGSHGIRWENGLVYDGQLLRLAVDLADRLLPAFYTETGLPYPRVNLRRGVPFYDNSPLNAAASCDGPGCRKRRFPMETTETCSAGAGSLVLEFTVLSRLVGDGRYEELGKRAFWAVWNRRTDIGLIGAGIDAESGRWVHPYTGIGAGVDSFFEYAFKSYVLLSSGESPPYNRSSPWSTLDGHYRPLSEYEHSAEGFLHAWHDAHESIKHHLYRGEGYQHLHLIQGDVFTGAARAAWIDSLSAFYPGLLTLAGELDEAIEIHLLTTAVWTRFSGLPERWNVVTGNIEGGLSWYDGRPEFAESTYYLYRATRDPWYLHVGEMVLRDIKRRCWTKCGWAGLQDVRNGDLKDRMESFILGETAKYLFLLYDSGHPLNNLDKPFVFSTEGHPLIIPDSTTGNLGARKDRPHTQVSLTCQTAPLPPTFGISSTAARPDVFHAASLARLHLMPSREPVEGAILEYASDHPSVSISDLSSPTNYTYFPWTLPAELVPFNATSSPMTIRPAVDISFPAIPGLGMGPGSLERIGNGVLVKAIGGLRLSMAQDTLRQADKADPDEGFRVQVVNNVPLGKDE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein can be targeted to cytoplasmic vesicle lumen and perform mannosyl-oligosaccharide 1,2-alpha-mannosidase activity.2. Design the protein with a signal peptide incorporated.3. The Proton donor, active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "EFHRGTYTSQAQTKKLMRLSEIKLHDLEWIAAKTSVLYPDSYKYPSKQINELWENVLLCQFHDVLPGSCIEMVYKYEAVPMLHNVVKECTSLIDKTVQFV",
    "response#2": "EFHRGTYTSQAQTKKLMRLSEIKLHDLEWIAAKTSVLYPDSYKYPSKQINELWENVLLCQFHDVLPGSCIEMVYKYEAVPMLHNVVKECTSLIDKTVQFL",
    "response#3": "GGWVQNDEATATIDDVITQMTQGHQWLKDTLNYTIEYAWQIDPFGYSSSTPTIFSSMGIKGLIINRVSDDVKSYMKSAKEMEFIWKGSESLGEQSQMLVSTLNVHYDYPKHIDPKKDFSLEERVKGFTKYIKDLSNTRESSILMIPLGDDFRYSNAKNEFSVSKEWLKVIQDNKEKYNIKEIKYATIDEYFIALEDEFMNKLGKTC"
  },
  {
    "reference": "MKTGKFIVIEGLEGAGKSSTHQVVVNTLTEFGIHEVVFTREPGGTPLAEKLRYLIKHEAEELVTAKAELLMLYAARVQLVENVIKPALARGKWVVGDRHDLSSQAYQGGGRGLDRHLMTTLKNAVLGDFKPDFTLYLDIEPEIGLARAKGRGELDRIEQQNLDFFHRTRERYLQLVQQDPNAVLINAGQPIELVQQDIRKAVQKFIEENV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be designed to have high stability and activity towards ATP + dTMP = ADP + dTDP.2. The protein should be able to modulate phosphorylation in a way that leads to a desirable outcome.3. The protein should be able to bind ATP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "GIATGLTALDNLTGGFQNSELIVIGARPSMGKTALAMTMASNIAIRQRIPTAFFSLEMSNLLLMQRLIAAESGVSATNLRKGLLQLSDFGRIQNAAGEMYDAPLYIVDVPNMKLLDLRAVARRLCVQEKIQIIFVDYLGLIVADNPFAPRYEQFAAISQSLKSLARELDIPIVALSQVGRPAEGSAPNLADIRGSGAIEQDADVVMFLHRDRNETETQLILAKQRNGPIGTVELEFQASFTRFR",
    "response#2": "PMESTLLSKSLEAAQKKVEAYFYDTRKQVFEYDQVLNSQRQAIYAERRRILESGYPRDCILQYAESTIDDIVNFCLTSKENNEKFVNLNTKIKYR",
    "response#3": "PMESTLLSKSLEAAQKKVEAYFYDTRKQVFEYDQVLNSQRQAIYAERRRILESGYPRDCILQYAESTIDDIVNFCLTSKENNEKFVNLNTKIKYR"
  },
  {
    "reference": "MSRGSGAGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSVCVVTQKKVPDKLLDQSSVSHLFPVTKYLGLLATGMTADSRSLVQQARNEAAEFRFQYGYEMPADILAKWIADKSQVYTQHAYMRPLGVVAMVLGIDEERGPLLYKCDPAGHFYGHKATSAGMKEQEAVNFLEKKMKENPAFTYDETVQTAISALQSVLQEDFKATEIEVGVVRADDPLFRSLRTEEIDEHLTAISERD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH; The proteasome has an ATP-dependent proteolytic activity.2. The designed protein should be involved in ubiquitin-dependent protein catabolic process, proteasomal protein catabolic process by localizing to proteasome core complex, mitochondrion, nucleus, proteasome complex and exhibiting structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "VEKAELRLAIADSPQKFETNLQTFLPPLLLKLASPHASVRTAVFSALKNLISRINTLPQVQLPVRALIVQAKEPNLAAQQDSTNVRLYSLLLASKGIDRLSLQDRQQLLPLVVSNISCLTGTVAARMFHILLKLILEWVAPQESSHEQEEFVQFLQLDNDGFSFLMRQFTRFFLLVPSKQVQVSQQPLSRGYTCPGLSLTDVAFFTYDAGVTFNKEQLNKFKKAIFQFVCRGMAATQTIEQSPRMIELMEFLCVVSTDSTNLSDDAAQFMKRFPMPYENEEFITFLQTLYIGNTANGRPPVKAILQEKILSILNRSHFATTKAECISLICSIGLHSSEYKLRSLTLSFIRHVAKLNYKNLNPASSSPSSTDFSTCIVSLIRNNLHAEGWPKLQLGPQTPAFNTAILQRQLQYETLGDILKRDFELVSDLSYIEFLFESLKNDLPQFRSSIQESLLSLVGHLSILPQQSKLKLKNLLRKNLSIDEQQREDNNDAVNSIMALKFVSIKFTNAAFPFHDPEARLFNIV",
    "response#2": "VEKAELRLAIADSPQKFETNLQTFLPPLLLKLASPHASVRTAVFSALKNLISRINTLPQVQLPVRALIVQAKEPNLAAQQDSTNVRLYSLLLASKGIDRLSLQDRQQLLPLVVSNISCLTGTVAARMFHILLKLILEWVAPQESSHEQEEFVQFLQLDNDGFSFLMRQFTRFFLLVPSKQVQVSQQPLSRGYTCPGLSLTDVAFFTYDAGVTFNKEQLNKFKKAIFQFVCRGMAATQTIEQSPRMIELMEFLCVVSTDSTNLSDDAAQFMKRFPMPYENEEFITFLQTLYIGNTANGRPPVKAILQEKILSILNRSHFATTKAECISLICSIGLHSSEYKLRSLTLSFIRHVAKLNYKNLNPASSSPSSTDFSTCIVSLIRNNLHAEGWPKLQLGPQTPAFNTAILQRQLQYETLGDILKRDFELVSDLSYIEFLFESLKNDLPQFRSSIQESLLSLVGHLSILPQQSKLKLKNLLRKNLSIDEQQREDNNDAVNSIMALKFVSIKFTNAAFPFHDPEARLFNIL",
    "response#3": "VEKAELRLAIADSPQKFETNLQTFLPPLLLKLASPHASVRTAVFSALKNLISRINTLPQVQLPVRALIVQAKEPNLAAQQDSTNVRLYSLLLASKGIDRLSLQDRQQLLPLVVSNISCLTGTVAARMFHILLKLILEWVAPQESSHEQEEFVQFLQLDNDGFSFLMRQFTRFFLLVPSKQVQVSQQPLSRGYTCPGLSLTDVAFFTYDAGVTFNKEQLNKFKKAIFQFVCRGMAATQTIEQSPRMIELMEFLCVVSTDSTNLSDDAAQFMKRFPMPYENEEFITFLQTLYIGNTANGRPPVKAILQEKILSILNRSHFATTKAECISLICSIGLHSSEYKLRSLTLSFIRHVAKLNYKNLNPASSSPSSTDFSTCIVSLIRNNLHAEGWPKLQLGPQTPAFNTAILQRQLQYETLGDILKRDFELVSDLSYIEFLFESLKNDLPQFRSSIQESLLSLVGHLSILPQQSKLKLKNLLRKNLSIDEQQREDNNDAVNSIMALKFVSIKFTNAAFPFHDPEARLFNIA"
  },
  {
    "reference": "MLDSQVIATIKSTIPLIVSTGPKLTAHFYERMFNHHPELKDIFNMSHQINGDQREALFNALCAYAANIDNLSALLPAVEKIAHKHASLNIQPEHYQIVGTHLLATLDEMFHPSDEVLNAWGKAYNVLADVFIHREEEIYQSAESLTGGWRDLRPFRVSRKQPQSDVITSFEFVPVDGGEVMDYKPGQYLAIYVEDPTLENREIRQYSLTAAPNGIGYQIAIKREPHGKVSNHMLDKVQEGDIVMLAAPRGDFFLDVQPDTPVTLISAGVGLTPMMSMLQHLHHQHHAGTVNWFHAAEHGGHHAFANKVNEIAQSMPNLQSQVWYREPRDIDQQGTHYQHTGLMDLSFVRDAITAEGMQFYFCGPVAFMQHIAKQLLAMGIDEHCLHYECFGPHKVI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should contain one or more FAD-binding FR-type domains that are essential for its function.2. The protein should have a high affinity for NADP(+). The designed protein sequence is ",
    "response#1": "MLSAHTIAVVKSTIPLLENAGTAITQHFYQRMFNHNPELKDIFNMSNQQSGRQQFALFSAIAAYAKNIETPEVLATAVERIAQKHTSLNVQAHHYGIVGHHLIETLRELAPDAFTPEVEAAWTEAYGFLASIFRR",
    "response#2": "MLSAHTIAVVKSTIPLLENAGTAITQHFYQRMFNHNPELKDIFNMSNQQSGRQQFALFSAIAAYAKNIETPEVLATAVERIAQKHTSLNVQAHHYGIVGHHLIETLRELAPDAFTPEVEAAWTEAYGFLASIFE",
    "response#3": "MLSQKTIDIIKSTAPILETEGKKITTCFYKMMFEAHPELLNIFNHVNQKQGRQQTALANTVYAAAKYIDQLEVIVPTVTQIAHKHRGLGIKPEHYPIVGEFLLKAIKEVLGDAATDEIIGAWGEAYGVIAQAFIDVER"
  },
  {
    "reference": "MSISDPKKRSLNTQGYKAFLQKQYFRALFLFSQALFLEPLDAHAKIGLLLSDIALDFPKEAHSFYELYQNLLDSQPRRYKTSIQNQILDLINSFDESLSKMAQMFGQENQLKAESLDGILYADFKAMCAGKNFKEVFENLMFSTKVIFDKKEDFYEFLDNLVANQFYEMSISYIENMRELLWYDKRISAILQKALLLEKQALKASKKMKLAKEIDACGRVFSAISDDTRDLGDPHCLLLKTPSNQAFVQAHLEKFPNTPYIHASELKNHFDLGLKIIGITGTNGKTTTASLIYSLLLDLGYSCALLGTRGFFCNDQRLKPKGLTTPPLLELYSDIERAKQEGAGYFVMEVSSHAIAQERILGLEFSAKVITNITSDHLDYHKNLDTYRQVKNAFLQDTGLKVINRDDPHVRFNPTNAYGYGIEAKSHLSVSAYSLLPNLGAHVEFWENPSLRYRSLEKSTHEACLLHSPLMGLHNLYNLLGGLLCVKLLTKQSLESLSTLAQNFLGVQGRMEVAHSKPLVIVDFAHTADGFEQIFNSFKGHKIKALFGAGGNRDKSKRPLMGAIASQHAMQVYITSDNPRYEDPLTIMQEILAGIPEHKRVQVILEVDRKKAIILALEALKSDEVLLILGKGDESVQIIGDQNLPFDDREVVKTYFEERT",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a low Km value for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.2. The protein design must feature the Meso-diaminopimelate recognition motif motif.3. The protein design should consider the endogenous proteins and enzymes present in the peptidoglycan biosynthesis pathway to avoid interference with native metabolic processes.4. The designed protein should have UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, ATP binding, tetrahydrofolylpolyglutamate synthase activity, magnesium ion binding within cytoplasm to regulate cell wall organization, cell division, cell cycle, peptidoglycan biosynthetic process, regulation of cell shape.5. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan. The designed protein sequence is ",
    "response#1": "MKLKDLISIRNLVADSRQIKPGDTFVAYPGDTTDGRKFIPQAIAAGANAILWDPREGAPAGLVVIADPHPRRALALTAASFYHDQPRQAVALTGVTGTNGKTSVATFLRMIWQELGLPAANLGTTGVEGDFEAPLKHTTPEPITLHRTLRDATQAGVECVAMEASSHGLEQRRLDGVQLTAAGFTNFTQDHLDYHATFDDYFDAKAGLFARVLPEDGTAVINIDDMRGTDMAAIAKGRGQHVITVGRHGADLELLAQRFDATGQDIRFSFGGEIYQTRLNLIGGFQAENVLLAAGLAMATGLDPEAVARGIARSRGVPGRMQVMRDAERLFIVDYAHTPDALIQVLQTLKRHVSNDLWAVFGCGGDRDRGKRPLMTQAALDYADIALITSDNPRTENPEQIFADMKAGLDFGPHQVHEIHDRREAIKFAAAHAQNGDIVVIAGKGHEDYQVLADGTLHFSDRETAERALQARQGGSR",
    "response#2": "MKLSTLLQRIPHIVDGIEIRNLVADSRQIKPGDTFVAYPGDTTDGRKFIPQAIAAGANAILWDPRLVEGIRNLVADSRQIKPGDTFVAYPGDTTDGRKFIPQAIAAGANAILWDPRSPLSALASRLWGSPSSRLCLIGITGTNGKTTVATLVWNILTELGENAALLGTVEKKINKKSYESRLTTADPVELAKDMKLMSEEGCNYLVMEVSSHALDQKRTDGFSFAVAAFTNLTLDHLDYHGSMEAYASSKKRLFNKLDSTSWAVINGDDPRGAWMAGSTPAKILTFSFADKGLIKASLVSSSLTGLTLNVDGTTIQTPLIGKFNAYNCVQALLCLLALGFELSVLLEAAPRLQPVLGRMELAATREGGGAVFVDYAHTPDAISTALQALRPHVMGRIVAIVGAGGDRDTTKRPLMGAAAHAHADVVIVTDDNPRSEDPAAIRAAVMQGVPEAIEVGDRAEAILQGAAMLEPGDALLIMGKGHENYQEVAGERLPFSDRLIVQQLLQ",
    "response#3": "MNLCQILDRIRNLVADSRQIKPGDTFVAYPGDTTDGRKFIPQAIAAGANAILWDPRDGIRNLVADSRQIKPGDTFVAYPGDTTDGRKFIPQAIAAGANAILWDPRDPGRLAAQIAARFFPRQPETVAAVGVTGTNGKTTTTHLIEFLLQAAQKPCGLLGTIEYRIGEKRIPAANTTPEAVELQQLLAEMRAQGASHAAMEVSSHALEQHRVWGCEFDVAVFTNLTRDHLDYHGTMEAYGQAKALLFRGLGKFGVAVINADDPWGRRLARQIPEATLITYGIERASDVRAELVRTELGGMQARLVTPWGQGPIHSPLVGRFNLSNLLAAVGAALALGHSLEGCVQAIEALQPVP"
  },
  {
    "reference": "MTDFFKDIPAIRYEGPDTENEFAFRHYNPDEVVMGKTLKDHLRFACAYWHSFAWPGGDPFGGQTFERPWFGDTMELAKLKADVAFDMFERLGVPYFCFHDADVRPEGDTFAESTKNLEEITDYFAEKMDATGVKLLWGTANLFSHRRFMAGAATNPDPEVFAYSAATIKSCMDATRKLGGENYVLWGGREGYETLLNTDMARERAQAGRMLQMVVDYKHKTGFAGTILIEPKPQEPTKHQYDYDVATVYGFLKDFGLEGEVKVNIEQGHAILAGHSFEHELALARALGIFGSIDMNRNDYQSGWDTDQFPNNVPEMALAYYEVLRAGGFDTGGTNFDAKLRRQSLDPADLILAHVGGMDACAAGLKAAAAMLEDGKLEAAREARYAGWSEGLGQKLLTSDLAEISDLVIAKGINPQPRSGRQERLENLVNKYL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.2. The ideal protein would be one that plays a critical role in D-xylose metabolic process through magnesium ion binding, xylose isomerase activity within cytoplasm.3. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+) binding.4. We need a protein that can perform alpha-D-xylose = alpha-D-xylulofuranose with high efficiency. The designed protein sequence is ",
    "response#1": "WQDSSTQKECDEFDAFLGGADKLANATGSKAHHRFTGPQILRFQRKYPEVYKKTSRISLVSSFLASLFLGHIAPLDTSDVCGMNLWNIKQGAYDEKLLQLCAGPSGVEDLKRKLGAVPEDGGINLGQIDRYYIERYGFSSDCTIIPATGDNPATIQ",
    "response#2": "AMVSLGTSTTFLMSTPNYMPDPATHFFNHPTTAGLYMFMLCYKNGGLAREHIRDAINDKLGMAGDKDPWANFDKITLETAPMGQKKDSDPMKMGLFFPRPEIVPNLRAGQWRFDYNPADGSLHETNGGWNKPADEARAIVESQFLSLRLRSRGLTASPGQGMPAQPRRVYLVGGGSKNKAIAKVAGEILGGSDGVYKLEIGDNACALGAAYKAVWAR",
    "response#3": "LKEMMETSHVQLLWNTANMFTHPRYVHGAATSCNADVYAYAAAKVKKGLDIAKELGAENYVFWGGREGYETLLNTDMKLELENLSSFYRMAVEYAREIGFDGQFLIEPKPKEPTKHQYDFDAATTIAFLETYGLKDHFKLNLEANHATLAGHTFEHELRVAA"
  },
  {
    "reference": "MKCKVEKSKISGQIVCPSNKSYTHRAIFLASLAGNGSKVENVLLSADTMATVEACKKFGASIEIENSSIIVKNPIKFDKIVPEINTENSGTTIRIASGIASLFSEEITLTGDESLQKRPMQPLLDALSSIGAQCQSTDGKPPIKITGKISGGDVTIPGNFSSQFISALLISAPLTEKGINLSIKDNLVSKPYLDATIATMRKFGVSVQTLIPYKRYNISPQVYNAATFTVPIDFSSLALLLSAAVLNGDETVIKGNIGNLPQGDEVFIDILEQLGVTVNIGEDEIKIKSPEKLKGGRFDLSNSPDLLPPLTILALNSENPIEIVNVKHARLKETDRIAITSRELVKLGIKVQENEDGLILESTENLTGAELNSENDHRLFMAFCIAGMYVGNCVVTDPESVQVSYPDFVEEMNRIGARIQPE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be designed to localize specifically to cytoplasm in order to perform its role in chorismate biosynthetic process more effectively.2. The protein design should take into account the physiological conditions of the chorismate biosynthesis, Metabolic intermediate biosynthesis pathway.3. The 3-phosphoshikimate binding site should be located in a region of the protein that is accessible to the ligand.4. The protein's Proton donor, Proton acceptor must be carefully engineered to ensure that the amino acids involved in 3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-   carboxyvinyl)-3-phosphoshikimate + phosphate are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "KGEVNALPPDKSIAHRAALIGAVADGTTEISNFSGGFDNQSTLGVLQACGIAVHQEKLKGPNGLISRNVVIHAQGFWSLSRPEKDLMCNNSGSTMRMFAGILAAQPFESRLAGDSSLMKRPMQRVAHPLRLMGADVALSSDNTAPVAIRGTTHLRPIDYELPVPSAQVKSLVALAALHAEGESRVIETLPSRNHTELMLGLKVEALSDGRSAVIIPGRKTVPAKPFHIPGDPSAACFIIALGLLSAGSEIVVRDVCLNPTRTGYMGVLLGAGAEISMENIRFAGGEKIGDVLVRHSPSMKPLSISDPAVVANSIDEIPMLAVLSACATGAFELYNAAELRAKESDRISAVVDNLERIGFVCDEYADGFAVTGRKDVTPTGKVAIDSYDDHRIAMSFAIADRVVPMELDISDAKVIGVSFPDFFER",
    "response#2": "SEVKKTYELPGDKSVGHRSLLIGALPKGEYKIRNFPQSRDCLTTLKIMEELGVKVKVLKDYILVNSPGYENFKKKIDYIDCGNSGTTSRLIAGILAGVGVETNLVGDKSLSIRPMKRIVDPLNSMGANIEMEKDHMPLIFKGNGELKGIDYTMEIASAQVKSCILLAGFLSEGVTKVRELSPTRDHTERMLKYIEGNIKIENKEIEIENSTIKSKDIYVPGDISSAAYIIACAILGEDCEIILENVLLNENRRKYLDLLKKMGANLKYLEKNQCNGEHVGNILVKSSFLKGISIGKEITPYIIDEIPIISLIASFAEGKTIFENVEELKYKESDRIKAIMVNLKSLGVKTELVENNLIIYGGLSKINKEINIRTFNDHRIALTFLCSAMRNSEKTYIDNWDCVAISFPV",
    "response#3": "NGPLTGKVRVPGDKSISHRALILGALAVGETRISGLLEGEDVLNTAKSMQALGASVERTGDFAWKVQGVGVAGFAQPKAALDFGNSGTGCRLVMGAVAGCPISAVFDGDASLRSRPMRRILDPLEKMGARVVSGGEGGRLPLTLQGARDPLPITYKTPVASAQIKSAVLLAGLAAPGTTTVIESEASRDHTELMLKHFGADITSTKEGQHGRRITLVGQPELHGANVVVPADPSSAAFPVVAALIAEGSDVVLSDVMTNPLRTGLFTTLREMGASIEESEVRGDAGEPMAQLRVRASKLRGVEVPPERAPSMIDEYLVLAVAASFAEGTTIMRGLQELRVKESDRLEATAEMLRVNGVKVEVSGDDLVVQGRGHVPGGGTVATHMDHRIAMSALVMGCASDQPVTVDDTAFIATSFPDFIEP"
  },
  {
    "reference": "MRFQLFIYFYFTIVVIAGTNTIQQFSDAGDRLITSLRNLDNNGTYETLTAEKVPIIEGQIQNISAKYEQHTFILKGLEAVLNYKVKSLDNNERESLEIEYEKVEKALDAALNVSPFEYIKKFKEVSRGKVVNALENLSREQNRITINGGREDEKEKEAREKKKRLDRIKRILTVSLLELGLAQGVADLCAVAPFACLLGVTVGSIGFIFWLALIYNAIQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Ensure the presence of a signal peptide within the protein.2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.3. The protein should be optimized for stability and solubility when expressed in cytosol, membrane where it will be involved in ascospore wall assembly.4. The protein must exhibit the following characteristics: Involved in spore wall assembly.5. The Extracellular, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "ILYENQRGIMLCGVPYFSEKSLLNFDPAPWVDQHFVASPVSTKTATCPDPSWEWAWQRWYIDMSGDVDESGWQYGFSFTMNNWHGKPVSLHSFVRRRRWIT",
    "response#2": "ILYENQRGIMLCGVPYFSEKSLLNFDPAPWVDQHFVASPVSTKTATCPDPSWEWAWQRWYIDMSGDVDESGWQYGFSFTMNNWHGKPVSLHSFVRRRRWIG",
    "response#3": "GLMSVLVFWLGLMKIAEQSGLLDIFSRMCRPFISKLFPDIPPDHPAMGYILSNLMANFFGLGNAATPLGIKAMEQMKKLNGNRSEASRSMITFLAVNTSCITLIPTTVAS"
  },
  {
    "reference": "MLNKNIGYISPDYLYKYNSPINTEAILKIRQERQAKLFKFSHEKYYKAVQTIADIQSNFFDCSSAVIKVGHPEEISPGQKQRLYHCLQTFCPWKKGPFELFGVNIDAEWRSDWKWDRILPHISSIKNRKVADIGCHNGYFMFRMVDQQPELVIGFEPYSKHFWNFQLIQNIVKQKMLAFELLGVEHIHYYPQFFDTIFCLGILYHHTDPIGLLRKMRQALGPKGEVIIDCQGIPGDLPVALTPQKRYAQARGIWFLPTQSCLENWIGRAGFSDINCFFAAPLSVEEQRRTIWANIDSLPEFLDPHNPSLTVEGYPAPWRYYAIARK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design must be able to perform transferase activity, transferring alkyl or aryl (other than methyl) groups in order to contribute to tRNA wobble uridine modification.2. The carboxy-S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions.3. We are looking for a protein with broad substrate specificity for 5-hydroxyuridine(34) in tRNA + carboxy-S-adenosyl-L-methionine   = 5-carboxymethoxyuridine(34) in tRNA + H(+) + S-adenosyl-L-   homocysteine.4. For general function, the protein need meet that Catalyzes carboxymethyl transfer from carboxy-S-adenosyl-L- methionine (Cx-SAM) to 5-hydroxyuridine (ho5U) to form 5- carboxymethoxyuridine (cmo5U) at position 34 in tRNAs. The designed protein sequence is ",
    "response#1": "MTIIEESAKIPEAVKLPNGEIDPYIFEQYEKMIDRARFESHFPIIPCNLCGSQDGLQRNQMLRDLENWIKANMPGRKESIMRALTNVRPSHLADKDLYGFQALPANEHAEVASNNDELLKHEIAEFDFSPAARFDAPGFSIAN",
    "response#2": "MAXPXIXGXAPLGEXVXDXPXAXDXAAXPXVXKXFXRXPXAXLSAXA",
    "response#3": "MNYANLASGKKIILVGLSHKSGVESALEALHKENAEIVGFDINREFIGENIFDKIIKGDFSKPDVIKEYIKNKGIEAVVSMGGQTGLTLTTALSNEGIRVLGTKYETIEISEHRELFRKTLEEIGMPVAEYKTVHDFEDALEKVSYPLPVVIRPSFTLGGTGGGIAYNREELKEIVKRGLLVSPTTEVLIEESAIGWKEYEMEIMRDKNDCIVIMACENCDPMGIHTGDSITVAPIQTLTDKELSTIRNASFAVAKAIGVETGGTNIQFAISPETNEIYVLEANPRASRTVPFVAKATGIPMAKIATRVAVGAKLPELKQEFSVKPHFVSVKFPVFSFEKLEGVDIKLGPEMKSTGEVMGIDEDLEKAFYKSLEAASSYLPKKGAVLISVKDVKKEEAIPVAKKFYELGFDLIATRGTAKFLHDNGIKARIINKVSEGGNPDIVDLIKNGEIDFIINTTKGKRSIRDGVAIRKAAVKLKVPYITDIDTAMAIVKAIKNMENGELKVRSIQEYH"
  },
  {
    "reference": "MKQYTVAIVGATGAVGTQMLQCLEEQEFPVGELKLLASPRSAGKKVETPWGEVVIEAAAPECFDGVDIVLGAAEDDVAKALYPEAVHRGAVVVDNSHAFRLDEGVPLVVPEINAGDIASHHGIIANPNCATIIGLVPTWPLHQLAGVKRMIVSTYQAASGAGMPGMLELEAQIGAMGRGEEMPTPRAFAAQLASNLIPQIGGEKFEGFTSEEMKLQNEGRKIMHLPELRVNCTCVRVPVLRSHSESITLEFERDITPEQAREALAAAPGVKLVDDLEAESAHDRWPMPLDTSDQDLIYVGRVRRDISNPEALRGITFWCCGDQIRKGAATNAVQIAKLLTE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The Acyl-thioester intermediate, Proton acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.2. A protein with NADP binding, protein dimerization activity, aspartate-semialdehyde dehydrogenase activity, NAD binding is needed to enhance isoleucine biosynthetic process, diaminopimelate biosynthetic process, 'de novo' L-methionine biosynthetic process in a specific context.3. A protein should be able to manipulate the activity of enzymes in the L-threonine biosynthesis, L-threonine from L-aspartate: step 2/5, Amino-acid biosynthesis pathway, either by activating or inhibiting their function.4. The designed protein have Semialdehyde dehydrogenase NAD-binding domains with specific sequence motifs that are crucial for its activity.5. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.6. The protein should be able to bind NADP(+), phosphate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "VVAVVGATGLVGEILLRILEDRAFPVSELRPMASARSAGSKVAFRGQEWQVLEARADAFAGVDLVFFAATGALSRELAPAAVEAGATVIDKSSTWRLRDGVPLIVPEINADQIPTDAMVKADHKGGVKKIIFKVAVVGATGNVGREMLDILSERAFPADEVVAIASRRSAGQDVSFGDKILKVKALDSYDFSDTDICLMSAGGETSKEWSPRIGQSGCVVIDNSSAFRYHQDVPLIVPEVNPDAINGLKPLHDAASIKRVVVSTYQAVSGSGQKAITELETQVRKMFNMEDPEASVYPHRIAFNCLPQIDVFLDNDYTKEEMKMVNETVKIMGDHNIKVTATTVRVPVFYGHSESVNIEFENKLTAKEARVILTQAPGITVLDNPKEGIYPMPINSAGEDETFVGRIREDETIDNGLNLWIVSDNIRLNPIYRFSKINRVIVSTYQAVSGAGREGIDELSIQSKQVLDGSPIKPDKFAHQIAFNLIPHIDSWVEENYTKEEMKMVYETHKIFDDKEMAITATTVRVPVFRSHSESVYIETEENIPVEKVKELLSSSKGIVVKDEPQENSYPMPADTSDTDDIYVGRIRKDLYIKNGLNLWIAGDQIRYDNETGKSLWPLYEQIRIRRVVVSTYQAISGAGAAAMAELEQQTRDVLAGKAATPKELPHPIAFNLFSHNSAIGHDGYCEEEEKMIRETRKMFHDDDLRITPSCIRVPVPRTHSETLNIEFAEPMDEERVREILRSAPGVTLVDNREANHFPMPLEASGEDDVFVGRIRRDLSQPDGRGIDLFLCGDQLR",
    "response#2": "MNVAIVGATGAVGETLLAILAERQFPIGELHLLASERSAGEKLEYGARKLVVRDIAGFDPGGVDIALFAAGSSVSREYAAKFAAAGAVVIDNSSEFRGDADIPLVVAEVNPEALREAARTDRIVANPNCSTIQMVMALKVLHDQFGLKRLIVSTYQAVSGSGIEGVRELDEQVRAGVTQDLTALALDGSAVAFPEPSTYVAPIAFDVVAFAGNLVDDGSEETDEEQKLRNESRKILEIPDLAVSGTCVRVPVFTSHGLSINAEFEKPVTPQQARELLAKAPGVELADVPTPLQAAGTDPSYVGRIRQDQSVEGGCGLAMFVVGDNLRKGAALNSVQIAERLLAPLHRAATLKSVVITTLQAASGAGRDGVEELRAQHAALLAGEQPALAQVFGAQLADNVIPHCDALEADGYTREESKLLHESRKILGLPALPLTATCVRVPVEVGHSASMLVETERALEIEEARAALAAQPGVRVVDDTAAERYPTPREAAGTDEVLVGRIRKDRHDPRRLWVWQVADNVRG",
    "response#3": "MNLGVVGATGQVGAVVRQLLVDRAFPLASIRFFASARSAGTTIDFDGTPITVEDAATADPSGLDIAIFSAGATTSKSQAPRFAAAGVTVIDNSSGWRMDPEVPLVVSEVNPHAIDQARKGIIANPNCTTAIMVVPLNPLRAVAGIKRVVASTYQSVSGAGRGAMSELYDQTKAMFVNKKLEPSEFPRQISFNCIPHVGEFLEDGSTTEEWKMSAEAQKIMGEDIKIVATCVRVPVFVCHSQALNIEFHSAISVDAAYEALSEAPGVLVLGNESSMQYVTPIDCVDDDVVYVSRIRKDTTVPYGLSMWVISDNLRKGAALNAVQIAELLVK"
  },
  {
    "reference": "MKRSTPRPARSQAALTTSSEESPDRVERGGDGVATVTDFPDGPPDETGLTPRQRRILDVIRDSVRRRGYPPSMREIGEAVGLTSTSSVAHQLMVLQRKGFLRRDPNRPRAVEIRSAESAVPDASAGHSPAADRAPSARRPPRGPSPIDSNPDVVAVPLVGRIAAGGPALAEQLIEDVVPLPRQLVGEGTLFLLQVKGDSMVDAAICDGDWVVVRQQPVAENGDIVAAMIDGEATVKTFKRRGAHIWLMPHNPQYEPIPGDEATILGRVVAVLRRL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should possess a H-T-H motif domain that is capable of recognizing and binding to DNA in a specific orientation and spacing.2. We are looking for a protein with broad substrate specificity for Hydrolysis of Ala-|-Gly bond in repressor LexA..3. The protein should be able to modulate DNA replication, proteolysis, negative regulation of DNA-templated transcription, DNA repair, SOS response in a way that leads to a desirable outcome.4. The protein must exhibit the following characteristics: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA; In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.. The designed protein sequence is ",
    "response#1": "MEKLTPRQQMVLAFISAHLESHGYPPTLREIGGHLGINGTLGVMKHLDALERKGFITRNAGSSRRS",
    "response#2": "PIMGRIAAAVPISAIQQQINTLCLPQDMISLGEHYALEVKDDSMIEAGILDKDIIIVRRQNTATSGEIIIALIDKEEVTFKRYRRKGNSITLEAANPHYETRIYRPERVQIQGKLVGVI",
    "response#3": "PVIGKVTAGVPITAVENIEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMTEDDEATVKRFYKEKHRYRLQPENSTMDPIYLEQVTVLGKVVGVIA"
  },
  {
    "reference": "MSATSFSQVTVDTKANVYFDGKVVSHTVHFADGSKKTLGLIYAGEYHFGTAAAERMEIIAGSSEVTLDGQQETVHYASGTHFDVPANSGFTIAVSEGICEYICSFLS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must be able to perform uridine phosphorylase activity, thymidine phosphorylase activity under specific conditions.2. The protein must exhibit the following characteristics: Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates. The designed protein sequence is ",
    "response#1": "MXGXRXFGX",
    "response#2": "MTPKTCSPTK",
    "response#3": "LSSS"
  },
  {
    "reference": "MGFLTLFHMAFLAVSLFVSGALAAFGYTTSGNNFVIDAGSANPLIFSVSKSSCDINSIRYRGTELQYSKQGSHIGSGLGKATVSVSQINGSKSKFIKVTCVTSTLTQYMIVKEADSTIYMATYITAEPAIGELRFIARLLSDKLPYEYPYGEVSTTKGSSSTVEGSDVFVVNGQTRSKFYSSTRFIDEDSHCVYGGSDLTHVCIITPQQESSSGGPFFRDIDSNNAGESTNLYNYMNSGHVQTEDRRMGLHGPYLMTFSRSGIPKLKTVDISWFGELGVTGYVPDSQRGTVIGRATGIPSGFEGVVHWYNAAAQYWVRTAPNGDFTSPKMKPGTYTMVLYQTEFKVATSTVTVSAGKTTTASIASTFNTSHTTLFKIGEYDGQPTGFRNADKFLRMHPSDSRMSSWGPLTYTVGSSSLNDFPMAVFKSVNNPVTIKFNLGSAPSQATTLRIATTLSFAGARPQVVVNGWSAPAPAAPAKIDSRGVTRGAYRGYGEVYDVAVPAGKLISGTNTITISALSGSSGATFLSPNFIFDAVELFY",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a fast turnover rate for Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-   (1->4)-alpha-D-galactopyranosyluronic acid bonds of   rhamnogalacturonan I domains in ramified hairy regions of pectin   leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-   unsaturated D-galactopyranosyluronic acid at the non-reducing end..2. The protein must exhibit the following characteristics: Could be a pectinolytic enzyme that hydrolyzes the alpha-L- rhamnopyranosyl-(1,4)-alpha-D-galacturonopyranosyl glycosidic linkage by beta-elimination, thereby generating oligosaccharides terminating at the non-reducing end with a hex-4-enopyranosyluronic acid residue..3. Design the protein with a signal peptide incorporated.4. The designed protein should be involved in cell wall organization by localizing to extracellular region and exhibiting rhamnogalacturonan endolyase activity, carbohydrate binding, carbon-oxygen lyase activity, acting on polysaccharides. The designed protein sequence is ",
    "response#1": "DNAALTPMDVGSNKSIVGVGNKGVIKGKGLRVRNGNKNVIIQNIHITNLNPQYVWGGDAITLDNADQVWIDHNKISLIGRQFIVSGWGKAGHVTISNNEFDGRTSWSAGCNGKHYWTLLLLGEQDYYTFEGNWLHDVSGRAPHMGTDHTKSQIFFHGVNNYFQNLGGHAFDVDTNTWVLLEGNYGPAEDTSLPVWRLGTPDKSSGEFLHGDAPDPTHPLHPPQHFIYWGAYDWQQDFPNGVNYTIGSSDPAVDFNTVHWSVYGPTPANPDVEYDTTHDWTINFSLDKKQLQQRKTATLTIQLAGAKTAAGNTDVYNATEPYANLALESYINEQKEPLTLLVGFNQSSSCIVRSAVSCYQVRSRMEFPADWLNVGNNVLTLHLPRNATDYETAVLPGTVYVQYDALRLAQLP",
    "response#2": "TAGDTVTVTDLAGLTEAAENETPLTIIVSGAISGSAKIRVASDKTIYGETGSSITGVGFYIRRVSNVIMRNLKIGQVDADNGDAIGIDESTNVWVDHCDLSGDLSAGKDDLDGLLDITHGAEWITVSNTYFHDHWKGSLIGHSDSNEGEDLGHLHITYANNYWYNVNSRTPSIRFGTVHIINNYWDNLLLTGVNCRMDAQVLVQSSAFSNCPDVWRLGTPDKSSGEFLHGDAPDPTHPLHPPQHFIYWGAYDWQQDFPNGVNYTIGSSDPAVDFNTVHWSVYGPTPANPDVEYDTTHDWTINFSLDKKQLQQRKTATLTIQLAGAKTAAGNTDVYNATEPYANLALESYINEQKEPLTLLVGFNQSSSCIVRSAVSCYQVRSRMEFPADWLNVGNNVLTLHLPRNATDYETAVLPGTVYVQYDALRD",
    "response#3": "AINLHSWCDNYQASAPKVSVTDKAGILPITVNSNKSIVGQGTKGVIKGKGLRVVSGAKNVIIQNIAVTDINPKYVWGGDAITVDDSDLVWIDHVTTARIGRQHIVLGTSADNRVTISYSLIDGRSDYSATCNGHHYWGVYLDGSNDMVTLKGNYFYNLSGRMPKVQGNTLLHAVNNLFHNFDGHAFEIGTGGYVLAEGNVFQDVNIKNGQIVYIQSVNDLKTHLGGSTPKILVLQNDISASSKTTVTIGSNKTLVGSYAKKTLKNIYLTTSSASGNVIFQNLTFEHSPQINGNNDIQLYLDSGINYWIDHVTFSGHSYSASGSDLDKLLYVGKSADYITISNSKFANHKYGLILGYPDDSQHQYDGYPHMTIANNYFENLYVRGPGLMRYGYFHVKNNYSNNFNQAITIATKAKIYSEYNYFGKGSEVWRLGTPDKSSGEFLHGDAPDPTHPLHPPQHFIYWGAYDWQQDFPNGVNYTIGSSDPAVDFNTVHWSVYGPTPANPDVEYDTTHDWTINFSLDKKQLQQRKTATLTIQLAGAKTAAGNTDVYNATEPYANLALESYINEQKEPLTLLVGFNQSSSCIVRSAVSCYQVRSRMEFPADWLNVGNNVLTLHLPRNATDYETAVLPGTVYVQYDALRS"
  },
  {
    "reference": "MRLGVFGATGQVGRVMRTLLEQRNFPADEVRFFASSRSAGTTLPWAGGEVVVEDTATADFSGLDIALFSAGGAASKQFAPQVAAAGAVVIDNSSAWRKDPQVPLVVSEVNPEDVNERPKGIIANPNCTTMAAMPVLKPLHDEAGLRRLVVATYQAVSGSGLAGVRTLTDQTQATRDPAELALDGAAYQPTDLGPYVAPIAYNAVPIAGNLVDDGTGETDEEQKLRNESRKILHIPDLLVAGTCVRIPVFSGHGLVVHAEFADEITPQRATELLRAAPGVEVVDVPDPRSAAGTDPSLVGRIRADQSAPEGHGLVLFCTADNLRKGAALNAVQLAELVVAQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein should be able to manipulate the activity of enzymes in the L-threonine biosynthesis, Amino-acid biosynthesis pathway, either by activating or inhibiting their function.2. The protein design should be able to enhance the efficiency of lysine biosynthetic process via diaminopimelate.3. The protein should have a fast turnover rate for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH. The designed protein sequence is ",
    "response#1": "MKVMKFGGSSLADAERYLRVRDIVLHTHTEKGAAVVLSAPKGVTNALSLLVEQAVAGDPWQELFEKLNMTLTGIVKDLANELDNFDHQPLLTLIDEKLLELKQSLEGIHLLHCSPDAVTAKLLSIGEYVSVNIFSGIISAMGVSNQIIDPVEYVLAEGDYLDSIADVAASRARFTNVPKNGEQMLIMPGFVAANEKGEKVTLGRNGSDYSAAILAACIDAQCCEIWTDVDGVYNADPNQVDGAVLLDKLTYQEAMELSYFGAKVLHPKTIGPIAQYHIPCLIRNTLNPSAPGTLIV",
    "response#2": "LKPLMKFGIEKIYLASMQAISGAGYDGVASMAILDNVIPYIDKEEEKVETETKKLLGEFNGSEIVDAPFAVSASCNRVMVMDGHTEAVWVEMADNPSTEDIKQAFLEFDPGLSDLPTEPAPVIEVKEEKDRPQPRMDRNLGGGMTVSVGRIRPGIRYICMGHND",
    "response#3": "MKVMKFGGSSLADAERYLRVRDIVLHTHTEKGAAVVLSAPKGVTNALSLLVEQAVAGDPWQELFEKLNMTLTGIVKDLANELDNFDHQPLLTLIDEKLLELKQSLEGIHLLHCSPDAVTAKLLSIGEYVSVNIFSGIISAMGVSNQIIDPVEYVLAEGDYLDSIADVAASRARFTNVPKNGEQMLIMPGFVAANEKGEKVTLGRNGSDYSAAILAACIDAQCCEIWTDVDGVYNADPNQVDGAVLLDKLTYQEAMELSYFGAKVLHPKTIGPIAQYHIPCLIRNTLNPSAPGTLISTEASDTWTSVKGISHLSDITMFNVAGPGMKGMVGMASRVFEVISNANISISLITQSSSEYSISFCVHAKDAQRAKHLLEEAFVLELANQLLEPIEMRESVAIVTLVGDGMRKAKGLAARFFSSLAQARVNIVAIAQGSSERSISAVIESRKAREAVKVVHQNFFSNLHTIDAFLVGCGTVGSELLEQIARQQQQLLGRNINLQVYGIANSKGMLLQPGGIDLKNGWRDQLAQRQQPFSLAVMENFVQDNSLINPVIIDCTTSTAIADQYLDMMEAGFHVVTPNKKANTAGMDFYAALREKAQSTRRQFLYETTVGAGLPVIENLQKLLYAGDELTRFEGILSGSLSYIFGKLEEGLSLSEATKIAREKGYTEPDPRDDLSGMDVARKLLIMARESGLKLELKDIEIESILPDDFDDSGSVEEFLSRLPQLDQLYKQQLEQNPGKVLRYVGSIEEGHCKVGIQAVDNSHPLH"
  },
  {
    "reference": "MTPLLTLFLVVLMGLPLAPVQALDCHVCAYNGDNCFNPMRCPAMVAYCMTTRTYYTPTRMKVSKSCVPSCFETVYDGYSKHASTTSCCQYDLCNSAGLAIPATLALAPVLLATLWGLL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Reduces cell surface expression of (alpha-3:beta-4)(2):beta-4 and (alpha-3:beta-4)(2):alpha- 5 nAChRs suggesting an interaction with nAChR alpha-3(-):(+)beta-4 subunit interfaces and an allosteric mode; Corresponding single channel effects characterized by decreased unitary conductance, altered burst proportions and enhanced desensitization/inactivation seem to depend on nAChR alpha:alpha subunit interfaces and are greater in (alpha-3:beta- 2)(2):alpha-3 when compared to (alpha-3:beta-2)(2):alpha-5 nAChRs; Enhances desensitization by increasing both the rate and extent of desensitization of alpha-4:beta- 2-containing nAChRs and slowing recovery from desensitization2. The protein should have acetylcholine receptor binding and be active in endoplasmic reticulum, dendrite, membrane, plasma membrane. The designed protein sequence is ",
    "response#1": "LECHVCAYNGDNCFKPMRCPAMATYCMTTRTYFTPYRMKVRKSCVPSCFETVYDGYSKHASATSCCQYYLCNGAGFATPVTLACN",
    "response#2": "LDCHVCAYNGDNCFNPMRCPAMVAYCMTTRTYYTPTRMKVSKSCVPRCFETVYDGYSKHASTTSCCQYDLCNGTGLATPATLCA",
    "response#3": "EDRLFKHLFGGYNRWARPVPNTSDVVIVRFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWDPAEFGNVTSLRVPSEMIWIPDIVLYNNADGEFAVTHMTKAHLFFTGTVHWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLEQMERTVDLKDYWESGEWAIINATGTYNSKKYDCCAEIYPDVTYYFVIRRR"
  },
  {
    "reference": "MNIHTIILAAGEGSRMKSKRAKSLQRVGGKTMLEMICKTAGSISTKITLIVGFDKDSIINESKNFDFEISTCEQASPIGTGDAVKCGINEVDDADKVLVLYGDVPLIKKETLENLVSTDDKSLTILSTKVDNPFGYGRVKKDNDGNVTQIIEEKDASNADKKISEIFTGILCCNKVLLAEALEEIKNDNAAGEYYLTDIVSIINKKGFKIKTCIVSNEEVKGANTKKELSELEETFRKMKSKDLLEMGITVSDPNRVDVRGEVSAGTDCIIDVNVILDGVVKLGDNVSIGPNTILKDVEIGNNTSIEAFSHLVSSKVGKDCIIGPYARLREGSQIDDSAKIGNFVETKKTTVGKGSKASHFAYLGDAEIGSGSNIGAGTITCNYDGKDKHQTKIGDESFVGTNSSLVAPVTIGSNAYVAAGSVITKDVPDNALGVGRSKQNNVEDWSKNKD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The ideal protein would be one that plays a critical role in peptidoglycan biosynthetic process, cell morphogenesis, cell wall organization, regulation of cell shape, lipid A biosynthetic process through magnesium ion binding, glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity within cytoplasm.2. Incorporate a signal peptide in the protein design.3. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.4. The Proton acceptor of the protein must be engineered to enable effective catalysis of acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.5. The presence of MobA-like NTP transferase domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MTLALILAAGEGTRMKSALPKVAHRILGVPLVRYVIDAAHTAGCEQVVVVTGHGAETVEALVGAETCVRQEEQLGTGHAVMCAEKALEGLTGALVVLSGDSPLLRPETIARLVDARETSRAVAVVLTTTLADPAGYGRIVRDELGKLLGIVEEKDATPEQRGVTLADPARIDVRGTLTCGRDVFIDVNCVFEGDVTLADNVTIGPNCVIRNAAIGAGTRIDAYTHIEDASVGAQAVLGPYARLRPGAKLADEVHVGNFVEVKNANIGHGSKANHLSYVGDADIGAGVNIGAGTITCNYDGANKHRTVIEDNVFVGSDTQLVAPVRVGRGVTIAAGTTVWKDVADDMLVLNEKSEVWFEGWRKRKAA",
    "response#2": "VILAAGEGTRMKSALPKVLHPIAGLPIVAHVAKAVHGAGGSDIALVVGRGAEKVEAAVRAVVSNVSVHEQTERLGTGHAVLAARNAIDAGYDDVLVVFGDTPLVETAALEQARAKLASGADVVVMGFEAADPGRYGRLVTDGDRIVEYKEATDEQRAISLCNSGVICAEARLLFELVGRVGNNNAAGEYYLTDIVALGRAAGHRATVVRCPEAETMGVNDRAELAEAEARWQARRRVRAMADGATLIAPETVWFAWDTVLGRDVTIEPNVWFGPGVVVADSVTIRGFSHIEGARLAEGVEVGPYARLRPGAVLEAGAKIGNFVEVKNATLGPGAKANHLTYLGDAEVGAGANIGAGTITCNYDGYFKHKTVIGERAFIGSNSALIAPVRIGADSIVAAGSAVSRDVGDGELRLVRAEKPQKPGLAKTLFDMLKAKKAKARKS",
    "response#3": "VVIMAAGKGTRMRSARPKVLHTLAGRSLLEHVLGRAARLQALQTLVITGHGAEQVEASVGAAAQCVRQQPQLGTGHAIQQVAPVLDEAAATTLILNGDVPLIEPATAAALAQACGGQRLALLTVELDDATGYGRIVRDAAGQVRAIVEHKDATEQQRTIREVYTGMMAAPTAMLKRWVTALDNNNAQGEYYLTDIVAMAVADGVPVVAHRITDALQVAGVNSPAQLAELERAHQLRQAEALMEQGVTLVDPDRIDIRGSVSAGHDCVIDVNVVFEGEVSLGEGVNIGPGCVIRDSVLGDGVRVEPMSAIDGASVAAGCRIGPFARLRPGTELGESVHIGNFVETKKASLGAGTKANHLAYLGDASVGEGSNIGAGSVTCNYDGVDKHRTEIGDGVFVGTNATLVAPLVVEDEAYIGAGSTITTKVGRKDLAVGRGRQRNIRGWQRPVKQPKA"
  },
  {
    "reference": "MELEVPDEAESAEAGAVTAEAAWSAESGAAAGMTQKKAGLAEAPLVTGQPGPGHGKKLGHRGVDASGETTYKKTTSSTLKGAIQLGIGYTVGNLSSKPERDVLMQDFYVVESIFFPSEGSNLTPAHHFQDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSVFYVTSDDEFIIKTVMHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQSGGKNIRVVVMNNVLPRVVKMHLKFDLKGSTYKRRASKKEKEKSLPTYKDLDFMQDMPEGLLLDSDTFGALVKTLQRDCLVLESFKIMDYSLLLGVHNIDQQERERQAEGAQSKADEKRPVAQKALYSTAMESIQGGAARGEAIETDDTMGGIPAVNGRGERLLLHIGIIDILQSYRFIKKLEHTWKALVHDGDTVSVHRPSFYAERFFKFMSSTVFRKSSSLKSSPSKKGRGALLAVKPLGPTAAFSASQIPSEREDVQYDLRGARSYPTLEDEGRPDLLPCTPPSFEEATTASIATTLSSTSLSIPERSPSDTSEQPRYRRRTQSSGQDGRPQEEPHAEDLQKITVQVEPVCGVGVVPKEEGAGVEVPPCGASAAASVEIDAASQASEPASQASDEEDAPSTDIYFPTDERSWVYSPLHYSARPASDGESDT",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The ideal protein would be one that plays a critical role in regulation of postsynaptic neurotransmitter receptor internalization, axonogenesis, endocytosis through ATP binding, 1-phosphatidylinositol-4-phosphate 5-kinase activity within uropod, cytosol, plasma membrane, adherens junction.2. strand-helix is the desired arrangement for the engineered protein.3. The designed protein must have at least one PIPK domain.4. The protein must exhibit the following characteristics: PIP5K1A-mediated phosphorylation of PtdIns(4)P is the predominant pathway for PtdIns(4,5)P2 synthesis ; Has a role in growth factor-stimulated directional cell migration and adhesion; Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2); Required for uropodium formation and retraction of the cell rear during directed migration; Required for clathrin-coated pits assembly at the synapse ; Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor; Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T- cell receptor; Mediates RAC1-dependent reorganization of actin filaments; Promotes particle attachment by generating the pool of PtdIns(4,5)P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. The designed protein sequence is ",
    "response#1": "SSGSYFVKDPKGKIIGVFKPKSEEPYGHLNPKWTKYFHKVCCPCCFGRGCLIPNQGYLSEAAASLVDQKLGLWIVPKTKVVHLASETFHYNAIDRAKSRGKKYALEKVPKVGRRFHRVGLPPKVGSFQLFVEGYHEADFWLRKFEAEPLPENMRKQLQSQFERLVVLDYVIRNTDRGNDNWLIKYEKPGDGELTEKESEWTDPKDSAIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVAFSQETRDLVLSRISDMNFVQDLCEDLYEMFRTDKGFDKTMFEKQMSVMRGQIGVFPERISQGSSGSYFVKDPKGKIIGVFKPKSEEPYGHLNPKWTKYFHKVCCPCCFGRGCLIPNQGYLSEAAASLVDQKLGLWIVPKTKVVHLASETFHYNAIDRAKSRGKKYALEKVPKVGRRFHRVGLPPKVGSFQLFVEGYHEADFWLRKFEAEPLPENMRKQLQSQFERLVVLDYVIRNTDRGNDNWLIKYEKPGDGELTEKESEWTDPKDSAIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVAFSQETRDLVLSRISDMNFVQDLCEDLYEMFRTDKGFDKTMFEKQMSVMRGQILNLTQALKDGKSPIQLVQMPRVVVEPLRTGMLELRGHKAKVFAALSPGELALYKSEQAFSLGIGICFIELQGCSVRETKSRSFDLLTPHRCFSFTAESGGARQSWAAALQEAVTESEFLTDLMMSEVDRCGDNEHLIFRENTLATKAIEEYLKLVGQKYLQDALGEFIKALYESDENCEVDPSKCS",
    "response#2": "LMGFIKLKLNRYAIIANTVEETGRFNGHVFYRVLQHSIVSTKFNSRIDSEEAEYIKLLELHLKNSTFYFSYTYDLTNSLQRNEKVGPAASWKTADERFFWNHYLTEDLRNFAHQDPRIDSFIQPVIYGYAKTVDAVLNATPIVLGLITRRSIFRAGTRYFRRGVDKDGNVGNFNETEQILLAENPESEKIHVFSFLQTRGSVPIYWAEINNLKYKPNLVLGENSLDATKKHFDQQKELYGDNYLVNLVNQKGHELPVKEGYESVVHALNDPKIHYVYFDFHHECVP",
    "response#3": "SSGSYFVKDPKGKIIGVFKPKSEEPYGHLNPKWTKYFHKVCCPCCFGRGCLIPNQGYLSEAAASLVDQKLGLWIVPKTKVVHLASETFHYNAIDRAKSRGKKYALEKVPKVGRRFHRVGLPPKVGSFQLFVEGYHEADFWLRKFEAEPLPENMRKQLQSQFERLVVLDYVIRNTDRGNDNWLIKYEKPGDGELTEKESEWTDPKDSAIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVAFSQETRDLVLSRISDMNFVQDLCEDLYEMFRTDKGFDKTMFEKQMSVMRGQIWEVSTSPRHAYGVFPERISQGSSGSYFVKDPKGKIIGVFKPKSEEPYGHLNPKWTKYFHKVCCPCCFGRGCLIPNQGYLSEAAASLVDQKLGLWIVPKTKVVHLASETFHYNAIDRAKSRGKKYALEKVPKVGRRFHRVGLPPKVGSFQLFVEGYHEADFWLRKFEAEPLPENMRKQLQSQFERLVVLDYVIRNTDRGNDNWLIKYEKPGDGELTEKESEWTDPKDSAIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVAFSQETRDLVLSRISDMNFVQDLCEDLYEMFRTDKGFDKTMFEKQMSVMRGQILNLTQALKDGKSPIQLVQMPRVVVEDKEPLLGPWKTLKKILSSGVFYFSWPNDGSRFDLTVRTQKQGDDSSEWGNSFFWNQLLHVPLRQHQVSCCDWLLKIICGVVTIRTVYASHKQAKACLVSRVSCERTGTRFHTRGVNDDGHVSNFVETEQMIYMDDGVSSFVQIRGSVPLFWEQPGLQVGSHHLRLHRGLEANAPAFDRHMVLLKEQYGQQVVVNLLGSRGGEEVLNRAFKKLLWASCHAGDTPMINFDFHQFAKGGKLEKLETLLRPQLKLHWEDFDVFTKGENVSPRFQKGTLRMNCLDCLDRTNTVQSFIALEVLHLQLKTLGLSSKPIVDRFVESFKAMWSLNGHSLSKVFT"
  },
  {
    "reference": "MSWQTILAIGSGGFIGAVLRAYFNGIISHKMPHDIPFGTLGVNLVGSFIMGILIAYFMYSTIFSLHVKSFLSTGVLGALTTYSTFAIESFLLLNSGHIALALANISLNAFGSILMAGGGFYIIKLSLRA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization.2. The designed protein should have a specific function within the plasma membrane. The designed protein sequence is ",
    "response#1": "KWNNISTSFVHSLITGVWSVLCFCMHPQMAEDLIETHSVFSHALVSVSIGYFIYDFLDMVINQKIIHSWELLFHHVVVITCFGISVLTCRYVGFAVVALLVEINSVFLHLRQVLRMANLAKSTFYRVNSMINLGTYVVFRINTLAWMTRWLVLNRDLIPLFSYTIGSVGLAIMTAMNIVLFYRLMY",
    "response#2": "KWNNISTSFVHSLITGVWSVLCFCMHPQMAEDLIETHSVFSHALVSVSIGYFIYDFLDMVINQKIIHSWELLFHHVVVITCFGISVLTCRYVGFAVVALLVEINSVFLHLRQVLRMANLAKSTFYRVNSMINLGTYVVFRINTLAWMTRWLVLNRDLIPLFSYTIGSVGLAIMTAMNIVLFYRLMY",
    "response#3": "RNAWKWNNISTSFVHSLITGVWSVLCFCMHPQMAEDLIETHSVFSHALVSVSIGYFIYDFLDMVINQKIIHSWELLFHHVVVITCFGISVLTCRYVGFAVVALLVEINSVFLHLRQVLRMANLAKSTFYRVNSMINLGTYVVFRINTLAWMTRWLVLNRDLIPLFSYTIGSVGLAIMTAMNIVLFYRLMRSDFMKAL"
  },
  {
    "reference": "MHVAVIIAAGGLGSRLNSRTPKQFLHVGEMSMLLRSIRTFEGHDLIDEIVTVLPSGYMEAYQGGLSSGETVFQVVAGGDRRQDSVANGFAHVSSNTDIVLVHDAARPFVGPEVISRTIAAATEFGAAIPAVAAKDTVKFGTTKDDQTVVASTLPRDEVYLAQTPQGFRRKVLEDAIAQGLESDATDESMLVERAGYTVRLVEGDPTNIKVTTKEDLALARLRCQDGNCSNETRVGIGYDLHRVEMGRPLILGGVNIPSDFGLSGHSDADAVCHAVVDAMLGAASCGDIGQHFPDSNAQWEGISSLDLMQRTYAMVYKQGFIVRNVDIVIIAEHPKIGPYVEGMRLNLAKVLSVELTRINIKGKTNEGVDAVGRGEAIAVQAVAMLEKMSVDVAASSENPDLE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D- erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C- methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF)..2. The protein should be able to bind a divalent metal cation, 4-CDP-2-C-methyl-D-erythritol 2-phosphate ligand in a variety of conditions3. The designed protein should be optimized for 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity, metal ion binding activity in order to improve terpenoid biosynthetic process, isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway.4. a divalent metal cation is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "RTGNGFDVHRFGPGDHVTLCGVRLPHGRGLQGHSDADVGMHAVTDAIYGALAMGDIGQHFPPTDPQWKGAESHIFLRHAVNLAAEHGFTITHADCTLICERPKIGPHAQEMRTVMAGIMGVGVDRISVKATTSERLGFTGREEGIAALATATAVLVAPDPDAERAAEAAGVRWVRVEPAPGAGTRSKLARLAHALGPDAVVGVGGDDTAAACALAAQLAGGARVGVGTDVHRWAPEDAPRPLWLGGVHWPGERGVAATSDGDVAAHAACNALLSAAGLGDLGAVFGVDRPEMADASGTAMLAEVLRLLTEAGLAVGNVAVQVVAPRPKVGTRRAEMEAALSAALGGAPVSVSAATTDRLGFIGRGEGLVGIATALEATGSTAALAAAASGFDVHRFGPGDHVWLCGVQIPHSHGLIGHSDADAGLHALTDALLGTIAAGDIGDHFPPSDPQWRGAASDTFLAHAATLVHAAGGIIDHADVTLICERPKIGPHRAAMRARIAAILGIDVARVSVKATTTELLGFTGRSEGLAAQAMAGVLVHDAAAG",
    "response#2": "IRLGNGYDVHRFGPGDHVTLCGISVPHDRGLQGHSDADVGLHAVTDALYGALAEGDIGRHFPPSDPQWKGAASDIFLRHAVELASSRGFSISNIDCTLVCEHPKIGPHAEAMRTRMAEITQIGRDRISVKATTSERLGFTGRGEGIAALATATRIGKGYDVHEFEAGDHVTLCGVAIPHIAKLKGHSDADAAWHALTDAILGAVALGDIGDHFPPSDPQWKGADSGLFLKEAQRLAEAKGYVIANCDITVICEAPKVKPHREAMRARTAELLGLPLDAVSVKATTTEGLGFTGRREGIAAEAVALLFG",
    "response#3": "RTGNGYDVHQLVAGDGVTLCGVRIPHDQTLLGHSDADVGLHALTDALLATCGGGDIGLHFPPSDPQWKGASSDRFLRHAAEMVSAKGGRICNVDVTLICEAPKIAPHRERMTRAIGNVLGLDADRVSVKATTNETIGFVGRREGIAAIATASTRAETILRSSMDIRLGNGYDVHRFGPGDHVTLCGISVPHDRGLQGHSDADVGLHAVTDALYGALAEGDIGRHFPPSDPQWKGAASDIFLRHAVELASSRGFSISNIDCTLVCEHPKIGPHAEAMRTRMAEITQIGRDRISVKATTSERLGFTGRGEGIAALATATLLRRRAEERTGLGYDVHAFEAGRPLILCGVTIPHDRGLAGHSDADVGIHTLCDAIYGALAEGDIGRHFPPSDNEWKDMDSARFLVHAGERIRARSGMLINADVTLICERPKIGPHAQAMRARLAELLQVDIDRISVKATTSERLGFTGREEGIAATAVATIRLGNGYDVHRFGPGDHVTLCGISVPHDRGLQGHSDADVGLHAVTDALYGALAEGDIGRHFPPSDPQWKGAASDIFLRHAVELASSRGFSISNIDCTLVCEHPKIGPHAEAMRTRMAEITQIGRDRISVKATTSERLGFTGRGEGIAALATATLER"
  },
  {
    "reference": "MESPETDLFKFDSIDDALADLKAGRAVVVVDDENRENEGDLICAAQFATPDMINFMAVEARGLICLALTGERLDALDLPLMVSKNTDSNQTAFTVSIDASPHLGVTTGISAEDRARTIQVAINPSTNAEDLRRPGHIFPIRAREGGVLKRAGHTEAAVDLPRLAGLYPAGVICEIQNPNGSMARLRELIDYAAQHQLKIISIADLISYRLKYDRFVHRETIARLPSIFGNFQIYAYRNTLDTSEHVAIVKGNPAEFRDNPVMVRMHSECLTGDGLGSLRCDCRMQLEAALKMIENFGQGVVVYLRQEGRGIGLINKLKAYSLQDLGLDTVEANERLGFPADLRDYGMGAQMLNDLGVKKIRLITNNPRKIAGLRGYGIEVVDRVPLLIEANDYNSTYLATKAQKLGHMLLQTYLVTVALQWQDAPQSVKKRYERLEKLRYLVHSQHLLLQEEARPVAIALFGTPSLIVHIGFDQSNLAAADWYKDSNHPYVQAIAHILDELSGWPQVERLEFLVSSGVDPMTGLQVQLDRQTFPLTKQPSDICDSLNTQKIYSFERDS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must have at least one GTP cyclohydrolase II domain.2. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein must have a Nucleophile; for GTP cyclohydrolase activity that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "VEVDMPTAYGHFRLIPFKGKTDGREHIALIKGDVADGEPVLVRVHSSCATGDIFGSRRCDCGEQLHRAMQMIEKEGRGVLLYLQQEGRGIGLMDKIKAYKLQEEGLDTVDANLHLGHKADERDYGVGAQILHKLGVKRMRLLTNNPRKRIGLEGYGLTVEENVK",
    "response#2": "ERVVETRLPTEYGEFTVMAYQSMVDDFVHIAVVMGKIDESVPVLVRVQSECLTGDVFHSLRCDCNAQLHASLKTISKNGSGIIVYMRQEGRGIGLVNKLKAYNLQDQGMDTVEANEALGFSPDLRHYGLGAQILVDLGVKQIRLLTNNPRKIVGLEGYGLFVAERVH",
    "response#3": "RSAETVLPTAKWGEWKVIAYKSDVGEDLHLALVKGEIHPEDNVLVRVHSECLTGDTFRSLRCDCGEQLERAMQMIAKEGKGVLVYLRQEGRGIGLLNKLKAYMLQDQGMDTVEANLALGFPPDLRDYGIGAQILIDLGVRRMRELTNNPKKIVALEGFELEITERVG"
  },
  {
    "reference": "MSKVITEEEIRKDVEEKNVRFLRLAFTDINGTLKNLEVPVSQLDDVLGNQTRFDGSSIDGFVRLEESDMVLYPDLATWLVLAWTTVEEGTIGRLVCSVHNVDGTPFEGDPRNNLKKVIAEMEEMGFSDFEIGFEAEFFLFKEGKNGEETTKVSDHSSYFDMASEDEGAKCRREIVETLEKLGFRVEAAHHEVGDGQQEIDFRFDNALATADKLQTFKMVVKTIARKYHLHASFMAKPVEGLAGNGMHTNMSLLKDGKNAFYDKDGQYNLSTTALTFLNGILEHARAITCVANPTVNSYKRLIPGFEAPVYISWASRNRSPMVRIPNANEVGTRLEMRSTDPTANPYLLLSACLKAGLTGIKEGKLPMAPVTSNLFEMTDDERKELGIKPLPSTLHNAIKAFKEDEVVKSAFSEHIVDSFLELKETEWALYTQSVSEWEVKRYFNY",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm that is critical for glutamine biosynthetic process.2. The protein should have a low Km value for ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine +   phosphate.3. The designed protein have GS catalytic domains with specific sequence motifs that are crucial for its activity.4. The protein must exhibit the following characteristics: In contrast, feedback-inhibited GlnA acts as a chaperone to stabilize the DNA-binding activity of GlnR, which represses the transcription of nitrogen assimilation genes.. The designed protein sequence is ",
    "response#1": "MSGIGKGITTASLAMLLKKMGYTVTIVKIDPYLNKDAGTMNPYMHGEVFVTDDGGETDLDLGHYERFLGINLSKENNITTGKIYGSVIEKERKGVFLGQTVQIIPHITNEIKDQLKDISKKYSSDVTFVEIGGTVGDIESLPFLEAARQMSIDLGKENVVYIHIAYIPLLPNTGELKTKPLQHSVNELRRIGIQPDMIFGRSTVPINEEIKSKISLFTNVPMNGVISTHDVDIIYEVPIMLKKEGADKYIVQKLGLKDKKPQLDEWRNLVKRIKNPKREVKIAMIGKYTKLHDSYLSIKEAIIHASAFYRIKPELIWIESTDIEEDKINLEEELNGINGGIILPGFGKRGVEGKIRAIKMLRENNIPLLGICFGMQLSVVEFARNVVGLEGAHSTEINPQTPYPVVSLLEEQMNVKYLGGTMRLGSYAIKLVKNTKVYDMYKQEIVYERHRHRYEINPKYIDKLIENGLTISGYSLEGNRVEFIEYNSNRFFVGTQSHPEYKSRPLTPSPVYLSFLKATLGK",
    "response#2": "MSGIGKGITTASLAMLLKKMGYTVTIVKIDPYLNKDAGTMNPYMHGEVFVTDDGGETDLDLGHYERFLGINLSKENNITTGKIYGSVIEKERKGVFLGQTVQIIPHITNEIKDQLKDISKKYSSDVTFVEIGGTVGDIESLPFLEAARQMSIDLGKENVVYIHIAYIPLLPNTGELKTKPLQHSVNELRRIGIQPDMIFGRSTVPINEEIKSKISLFTNVPMNGVISTHDVDIIYEVPIMLKKEGADKYIPRSTLKKVIKKCHEHQFDPVQGMEFEWYNYSENNKSLLNKNFSNLEPLSNGMFGYSLLRTSQNSEFMNSLAELQGFGVPLEGLHTETGPGVYEAAIRFSTALESADRAILFKHCTKEIASLQGIMASFMAKPFKDLPGCSGHMHQNFNCLKTGKNLFLDESDPNHMSDIFKSFVAGQLLLLPEFLPFFAPTINSYKRLVDGYWAPTTPTWGMDNRTVALRIIKGGKATRSEFRVTGSDVNPYISIAASFAAGLYGVINKLELKQKPIIGNSYDLYKKGLVERLPRSLAESTELLSKSKIAKEYLGEEFVDHFVETRRWEYRQFNHQVHKWELERYLEIKN",
    "response#3": "MSGIGKGITTASLAMLLKKMGYTVTIVKIDPYLNKDAGTMNPYMHGEVFVTDDGGETDLDLGHYERFLGINLSKENNITTGKIYGSVIEKERKGVFLGQTVQIIPHITNEIKDQLKDISKKYSSDVTFVEIGGTVGDIESLPFLEAARQMSIDLGKENVVYIHIAYIPLLPNTGELKTKPLQHSVNELRRIGIQPDMIFGRSTVPINEEIKSKISLFTNVPMNGVISTHDVDIIYEVPIMLKKEGADKYIERLTRVLSGSRFVAELLGRIPESVAWLESEEELRPRAPELLREETAAILARHESAETAAAALRAVRRREVLRLAFSGILGFSTIEELARGLSAVTENLLTGVLGAIRAARDDAAALEFAIIGMGRFGGRELGFGSDADIMYVFRPLAAGQDAAHERLTRVLSGSRFVAELLGRIPESVAWLESEEELRPRAPELLREETAAILARHESAETAAAALRAVRRREVLRLAFSGILGFSTIEELARGLSAVTENLLTGVLGAIRAARDDAAALEFAIIGMGRFGGRELGFGSDADIMYVFRPLAAGQDAAHARLVDVLSRSALLAERLAAYPLLLDELLDTRISGPLPDRAALHAACADIVHIDDTEAALRELNERRLARSFRIALATLDGRQQAVESTRQLAWLAEAVVQTVLHLARTEMVAAHGYVSGGSFAIIGYGSLGGMELGFGSDLDLVFLYDHPPEVDASDGKRPLEAGRWFARLAQKVMALLAAETGAGRLYDIDVRLRPDGGKAALVSSLASYREYQRERAWTWRLLRVIGASEGAAEFFLRQPVELASLHRPVTALPTAAELREDLLDAVGAVGGFASVAEEEAWTALRVRYRRRLVQLASFDLEQDDPVAGFDGVAAALSDLAGAALDASLAVARRQASGSGPGRFPEAEVRATRFAIIGMGKAGARELNYVSDVDVIYVTDGAEDAGVPPGRAVDIATRLAVLTQRGIQDPALEPGLWEVDSNLRPEGRDGALVRTLDSHLAYYDRWAKGWEFQALLKARSLAGDWELGE"
  },
  {
    "reference": "MVKQIRRYALFQATPDSEFVKEMYGGYFNVFVSAFGDEGEQWDLFRVIDGEFPRDEDLEKYEGFVISGSLHDAFTEEDWIIELCSVCKKLDVMKKKILGICFGHQIICRVRGGKVGRARKGPDIGLGNITIVQDVIKPGDYFDQIESLSIIQCHRDEVLEPPESARVIGFSDKCDVEIFSVEDHLLCFQGHPEYNKEILLEIIDRVHKIKFVEEEILEKAKDSIKKFEPDTQRLHMLCKNFLKGRRTH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be expressed specifically in the cytosol2. The designed protein must have a Nucleophile, active site active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "LPDAYLSVSEALRAGGFAHEARVNLRWVPSDECATPAGAAQHLGDVDAVCVPGGFGIRGIEGKIGALTYARKNGIPTLGLCLGLQCMVIEYARVEAGITDASSTEFDAATTSPVIATMAEQKSIVDGAGDLGGTMRLGLYPADLKAGSIVAEAYGAEHIEERHRHRFEVNNAYRDQLEAAGLVFTGLNPELDLVEFVELPRDVHPYYVATQAHPELRSRPTRPHPLFAGLVRAA",
    "response#2": "LPDAYLSVSEALRAGGFAHEARVNLRWVPSDECATPAGAAQHLGDVDAVCVPGGFGIRGIEGKIGALTYARKNGIPTLGLCLGLQCMVIEYARVEAGITDASSTEFDAATTSPVIATMAEQKSIVDGAGDLGGTMRLGLYPADLKAGSIVAEAYGAEHIEERHRHRFEVNNAYRDQLEAAGLVFTGLNPELDLVEFVELPRDVHPYYVATQAHPELRSRPTRPHPLFAGLVGAA",
    "response#3": "LPDAYLSVSEALRAGGFAHEARVNLRWVPSDECATPAGAAQHLGDVDAVCVPGGFGIRGIEGKIGALTYARKNGIPTLGLCLGLQCMVIEYARVEAGITDASSTEFDAATTSPVIATMAEQKSIVDGAGDLGGTMRLGLYPADLKAGSIVAEAYGAEHIEERHRHRFEVNNAYRDQLEAAGLVFTGLNPELDLVEFVELPRDVHPYYVATQAHPELRSRPTRPHPLFAGLVR"
  },
  {
    "reference": "MRRINTIMQGKTNGGNGPESGTVVVTRTQPKTRKPSLYRVLLLNDDYTPMEFVVHVLQRFFQKNLDDATRIMLHVHNHGVGECGVFTYEVAETKVSQVMDFARQNQHPLQCVMEKK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should aim to mimic or enhance the function of natural proteins involved in protein catabolic process, proteolysis, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "QLTIPIRVDQNGAFLSFTVKNDKHSRRRRSMDPIDPQQAVSKLFFKLSAYGKHFHLNLTLNTDFVSKHFTVEYWGKDGPQWKHDFLDNCHYTGYLV",
    "response#2": "QLTIPIRVDQNGAFLSFTVKNDKHSRRRRSMDPIDPQQAVSKLFFKLSAYGKHFHLNLTLNTDFVSKHFTVEYWGKDGPQWKHDFLDNCHYTGYLI",
    "response#3": "QLTIPIRVDQNGAFLSFTVKNDKHSRRRRSMDPIDPQQAVSKLFFKLSAYGKHFHLNLTLNTDFVSKHFTVEYWGKDGPQWKHDFLDNCHYTGYLY"
  },
  {
    "reference": "MTRTMISLFALAVASVALLGSAAMAADTVAGVDNTSNRFWWPEQINLAPLRAHGVQSNPMGAGFDYAAEFKSLDLAAVKRDINEALTTSQPWWPADYGNYGPFFIRMAWHSAGTYRIADGRGGAGGGQQRFEPLNSWPDNANLDKARRLLWPVKAKYGRKISWADLMVLTGNVALENMGVKTFGYGGGRTDDWEADLVYWGPEQKMLDDKRYSGDRTLAKPLAAVQMGLIYVNPEGPNGVPDPLASARDIRETFGRMAMNDEETVALIAGGHTFGKAHGAKSPEGCVGKEPAANPTERQGMGWDNKCGTGSGADTITSGLEGAWTASPVAWSMMYLENLFQFDWEKTKSPAGATQWIPANGQAAGTVPDAHVEGKFNTPIMFTTDLALKEDPAYRKVSQAFLKDPKKFEQAFAKAWFKLTHRDMGPRGRYIGNEVPKESLIWQDPVPAVDHPLVTAADVASLKGKVLASGLTTQELVRTAWASAASFRGTDMRGGANGARVRLAPMKDWAANNPAELTKVLGRLEAIQADFNKSASGGRKISLADMIVLAGGAAIEDAAKKAGHSVSVPFTPGRTDATVAQTDAESFAALEPKADGFRNFYTADSVYSPVEALVDRANLLTLTVPEMTALVGGLRALDANVGGGKHGVFTDKPGQLTNDYFVNLLGMETMWRKGGTEGLYEGVDRTSGKVKWTATPVDLAFGSHAELRAVSEIYGANDGADKFVADFVKAWDKVMSLGNFKG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein have enhanced AH2 + H2O2 = A + 2 H2O.2. The protein design must be able to perform heme binding, catalase activity in order to contribute to response to oxidative stress, hydrogen peroxide catabolic process.3. The designed protein have Plant heme peroxidase family profile domains with specific sequence motifs that are crucial for its activity.4. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "TFARAWFKLLHRDMGPKTRYLGPEVPKEDLIWQDPIPEGNKDFNISKAKELIKSADLTIQELIETAWASASTYRHTDMRGGANGARIMLSPQKDWDVNKPDQLRKVLNIYKDIAKKVNSSTADIIVLAGNVGIEKATGVDVPFIAGRGDALQEQTDIESFSVLEPMADGFRNFQKRDFSVSPEEMLLDKSQLIGLTAPEMTVLIGGFRSLGISSSGNEGVFTNDINTLSNDFFIDLLDMSIEWKPASKNTYDGFKRGTSEKVKTASRVDLVFGSNSQLRAIAEVYAA",
    "response#2": "AFARAWFKLTHRDMGPLSRYLGPEMPAEELLWQDPIPAVDHALVNEADIAALKSKLQASGLSVAQLVSTAWAAASTFRGSDKRGGANGGRLRLAPQKFWQANQPEQLTSVLETLEGIQAEFNQAQFGGKKISLADLIVLGGNAGIEQAAKNAGQTLSVPFAPGRMDASQEQTDVESFGFLEPAADGFRNYAKGRFSLSAEALLIDKAQLLTLTAPEMTVLLGGLRVLNTNYKQSPQGVFTHRPEALTNDFFINLLDMGTEWKPVSDAADEFEGRDRKTGEVKWTGTRVDLVFGSHAQLRALAEVYAE",
    "response#3": "TFARAWFKLLHRDMGPKTRYLGPEVPKEDLIWQDPIPEGNKDFNISKAKELIKSADLTIQELIETAWASASTYRHTDMRGGANGARIMLSPQKDWDVNKPDQLRKVLNIYKDIAKKVNSSTADIIVLAGNVGIEKATGVDVPFIAGRGDALQEQTDIESFSVLEPMADGFRNFQKRDFSVSPEEMLLDKSQLIGLTAPEMTVLIGGFRSLGISSSGNEGVFTNDINTLSNDFFIDLLDMSIEWKPASKNTYDGFKRGTSEKVKTASRVDLVFGSNSQLRAIAEVYAF"
  },
  {
    "reference": "MGQTSSHRQMHHSSNNNNKEEPPQPASQFGNRRTSTRKSTSSSKHYPTLVQPLRKDELSLLRDSLLRNGSIMRKNPERRPVTISELKPVNTGQGTSGSVLPITAEDAAGGSSSSITQLMAFQLPVDRSAKTDDLAAESSASAAAAVAPKEGRNEFYIVGDGYHRTDHCYYRSPNGDFHKLPSDSFHKMSEGCFVRMADGVFRRLEVPTKAQISRRSSRAEEIGDATSQGNGSAATNVKSQLMRFLKRSKSHTPATIAKLQREKEEARAERKQRLSNALRQKNLAATNAASAAAANSSYASGGGGGTVSSRNNKIVVTMIENGGLPIVATSKAERPKAKESASSSDKARNSRGSPNMQMRSSAPMRWRATEEHIGKYKLIKTIGKGNFAKVKLAKHLPTGKEVAIKIIDKTQLNPGSLQKLFREVRIMKMLDHPNIVKLFQVIETEKTLYLIMEYASGGEVFDYLVLHGRMKEKEARVKFRQIVSAVQYCHQKRIIHRDLKAENLLLDSELNIKIADFGFSNEFTPGSKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGSTLRELRERVLRGKYRIPFYMSTDCENLLRKFLVLNPAKRASLETIMGDKWMNMGFEEDELKPYIEPKADLADPKRIEALVAMGYNRSEIEASLSQVRYDDVFATYLLLGRKSTDPESDGSRSGSSLSLRNISGNDAGANAGSASVQSPTHRGVHRSISASSTKPSRRASSGAETLRVGPTNAAATVAAATGA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be able to bind ATP ligand in a variety of conditions2. The protein should have a specific function in oocyte microtubule cytoskeleton organization, axis specification and should be localized to neuromuscular junction, type I terminal bouton, cytoplasm, basolateral plasma membrane in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "KWRLFHCEMKSFNEWLTETEEKLSRAQIEAGDVGHVKTKQFLQELQDGIGRQQTVVKTLNVTGEEIIEQSSAADANVLKEQLGNLNTRWQEICRQLVEKRKRIEEEKNILSEFQEDLNKLILWLEETENVIAIPLEPGNEDQLRDCLGKVKLRVEELLPHKGILKRLNETGGTTLGSASLNPERKHKLESTLKEASRRLLKVSRDLPEKQKEIC",
    "response#2": "LRKDLSEMHEWITQAEEEYLERDFEYKTPEELQKAVEELKRAKEDAMQKEVKVKLITDSVNNFIAKAPPAANEALKKELDVLITSYQRLCSRLNGKCKTLEEVWACWHELLSYLDAENKWLNEVELKLKATENIQGGAEEISESLDSLERLMRHPEDNRNQIRELAQTLTDGGILDELINEKLEKFNTRWEELQQEAVRRQKSLEQSR",
    "response#3": "LRKDLSEMHEWITQAEEEYLERDFEYKTPEELQKAVEELKRAKEDAMQKEVKVKLITDSVNNFIAKAPPAANEALKKELDVLITSYQRLCSRLNGKCKTLEEVWACWHELLSYLDAENKWLNEVELKLKATENIQGGAEEISESLDSLERLMRHPEDNRNQIRELAQTLTDGGILDELINEKLEKFNTRWEELQQEAVRRQKSLEQSR"
  },
  {
    "reference": "MKNGEEVVISGIGGYFPKSINVEHFQKLLLENADFLDSRWKEGERGVTNVIGRVPGIQFFDTAYFGIHRQQCIHMDPMQRIMLERTFEALVDAGVNPVEIRGKKVGVFVGSSVGENDNIFYESVVSGFGVTGHSRSMMPNRISYWLNLKGPSCDYDSNWLNGLEVIRMAYESIREGHCDSAIIGTANLVVNSEISWTYNDAKLLSPDGITRSFDADANGYGRSDGIVVLYLQRATKAKRIYGTITNAKTCFNGNREGPLVAIDQPSMVKFMKDFYAETGVDPTEVHYVEAYGCGIKHVDETELNAIEEVYCKKRSGPLLIGSIKSNIGHAEASASLVAVVKVLISMNFGTIPANKHYKNPNPKIKGLLNGTLKVVDQNTPWSGDYAAVNAIGLASSYGHLLIKANKKTKKEGPADDLTRLFIASTRTEDGIQKILSTVQQHKNDAEYANLIQEIFATPIPGHLYRGYVLAGNEVKQEFEHYPGKKRPVWFVYSGMGSQWPTMASDLMRIPVFAESIMRSHAILQKKNVDLLNIITTNDPKIFDNILHSFIGIAAVQIALTDVLRTVGVVPDGIIGHSVGELGCAYADGCLTAEQMILAAYARGRASLEAELIPGMMAAVGIGYQEIKSQCPPTVEVACHNGPESCTISGPTKDMEDFVAQLKERGVFARLVNVANIAYHSRYIKPAAPFLLKYLKEIIPIASPRSSKWISTSIPEDRWDSDLAKTSSAEYHTNNLLSSVLFEEGSKHFPKDAIVIEIAPHGLLQAILK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must have at least one Enoyl reductase (ER) domain.2. The protein design must be able to perform 3-oxoacyl-[acyl-carrier-protein] synthase activity in order to contribute to fatty acid biosynthetic process.3. The protein must exhibit the following characteristics: This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.. The designed protein sequence is ",
    "response#1": "KPGSPGWYNTVAFQNEAKKAGIYAKSINGDAFSTEVKQKTIDLIKADLGQVDLVIYSLASPVRTNPVTGVTHRSVLKPIGGAFSNKTVDFHTGNVSTVTIEPANEEDVTNTVAVMGGEDWGMWMDAMLEAGVLAEGATTVAYSYIGPALTEAVYRKGTIGRAKDHLEASAATITDKLKSVKGKAYVSVNKALVTQASSAIPVIPLYISLLYKVMKAEGIHEGCIEQIQRLYADRLA",
    "response#2": "KPGSPGWYNTVAFQNEAKKAGIYAKSINGDAFSTEVKQKTIDLIKADLGQVDLVIYSLASPVRTNPVTGVTHRSVLKPIGGAFSNKTVDFHTGNVSTVTIEPANEEDVTNTVAVMGGEDWGMWMDAMLEAGVLAEGATTVAYSYIGPALTEAVYRKGTIGRAKDHLEASAATITDKLKSVKGKAYVSVNKALVTQASSAIPVIPLYISLLYKVMKAEGIHEGCIEQIQRLYADRLLDEKGRLRLDDWELRDDVQGACKALWPQVTTENLFELTDYAGYKKQFLNLFGFERADVNYDEDQYCEVLGSEVLCMGDRKSFFPGTQLNFSRILREFYNKFPVNPSELSYLEADGSTFMGNDREELNAVDEVLLKDRKTPLLIGSVKSNIGNTEASSSLCSIVKLIIAMTRKCIPPNLHQ",
    "response#3": "IFSGMGSQWPQMGTQLMQLPVFAESIRKCHATLEPLGVDLINIITTEDKSVFDNILNSFVGIAAIQIGLVDVLRKLEIHPDGLIGHSVGELGCAYADGCLTAEQMILSSYYRGKASLEAELIKGMMAAIGLGYEQIKDLLPPGIEVACHNSRGSCTLSGPTQAVEDFVAVLTAKGIFAKAVNVSNIAYHSTYIKPAAPLLLDYLKKIIPNPQPRSSKWICTSIQQKDWESDLGKTVSAEYLTNNLLSYVYFEEGSAHIPKNAICL"
  },
  {
    "reference": "MSVQGIEGVLQQLQVTALQASGSAKTLPAEAGFASELKAAIGKISENQQVARTSAQNFELGVPGVGLNDVMVNAQKSSVSLQLGIQVRNKLVAAYQEVMNMGV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that can perform structural molecule activity, cytoskeletal motor activity in bacterial-type flagellum basal body to support bacterial-type flagellum-dependent cell motility. The designed protein sequence is ",
    "response#1": "FRVMGTKPVAFLIAGFAKEREPFSVHFGAGGEMFLTTPLAVEPEKGLLIFDCSGSVESNQRFLRSDRNIFIGRPGGVHVQFTTGAAREVAHEGGKAFAVALPQYIVR",
    "response#2": "MFSRGGAELTPFFEDLLLALAPLFEQVTNPLIISGHTDAIPFKKRFGRQSNWALSASRADVARKTLVEGGMPDDRVMQVTGMSDRALLNPDEPDSSENA",
    "response#3": "FRVMGTKPVAFLIAGFAKEREPFSVHFGAGGEMFLTTPLAVEPEKGLLIFDCSGSVESNQRFLRSDRNIFIGRPGGVHVQFTTGAAREVAHEGGKAFAVALPQYIVG"
  },
  {
    "reference": "MCAVGTHSLPESLYAADGVRLLDRYAIEKCGIPGFDLMHQAARSAFRQLCRHWPDASTVAVLCGAGNNGGDGYLIAASAVRQGMTVLCFAVSPVTRLTGDALAAHHEAVETGVVVQAVDENDLSGLASQLSTADVIVDAMLGTGISGPLRAPFDQLVPTVNAAARPVLAVDVPSGLDASTGSVASDAVRAKVTVTFIGLKAGLLTGLGPDYVGDVLFDDLGVPDKAYATVEPVARRVDWSGMAADLPVRRRGAHKGDCGHLLVVAGERGFGGAGLLAAEAAARSGAGLVTLATRPEHVSAALARCPSLMVHGLTHGNDLDALLNSADGVVFGPGAGQGAWGQQMLQRIMQFNGPVLMDADALNMLSARAPGRKQNWILTPHPGEAARMLGEDTSAVMQDRLSAVVRLQKKYGGVVLLKGAGTLVYADTLAVPALVQGGNPGMATAGMGDVLSGIIGALVVQGMSVSDATITGASLHGEAADRAALETGFMGMLPMDLIDRIPRVLGEAEGVLIPLGFQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit NADPHX epimerase activity, ATP binding.2. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..3. The protein should be designed to have high stability and activity towards (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "MRQMDRQAIEHYGIFEDLLMENAGHAVYFMILEVFPVLEGKRFVVFCGLGNNGGDGLVVARKLHSNGARVNVFLLGDPEKYKGSARKNYEIVKGLSLPMKRLTKIGQAAYPLEHCDAVVDAMLGTGLSRPLEGLYRDVVLAINRAGKKVFSVDIPSGINGDTGDVMGVAVRADATATFGLPKLGNLLYPGFEYGGNLYVTHISFPPAIYRDAHKGNFGHVLVIGGDVGFAGAPLLAGEAAARVGCGLVTLATRPQHAGMQVAARPELMAWGVDRPAQLDPLLDRASVVAIGPGLGQSPWAAGLLARVLETNLPLVVDADALTLLARDPVQNERWVLTPHPGEAARLLDCSTAEIQGDRFTAVRELQSRYGGAIVLKGCGSLVATRKSGVTVCTDGNPGLASGGTGDVLTGVIAGLMAQGLEPESAALTGACLHGAAADRAVLEGERGMLAGDLL",
    "response#2": "PGAHKGNCGHVVVIGGAPGYSGAGRLAGMAALRAGAGRVTLVTHPEHAALANLERPELMVRPAESARDLQTLLAGADAVAVGPGLGQQAWGRALWPAVADWTGPLVVDADALNLLAQAPRRRDDWVLTPHPGEAARLLAVTTGEVTADRVAAVDAIRRRFGGVVLLKGAGTLVAPAPGGGMACITGGHPGMASAGMGDVLTGVMAALRAQGLGPGL",
    "response#3": "EGADLAPHLQQRPPDSHKGTFGHLLVVAGSEGMTGAAALAGEAAMRVGSGLVSVAVPQSLRTVIEMKLTECLKLGLEESEPGVLGEGAVRQLTPHLHRYHATLLGPGLSTRGEVARVVVALIERAEHPMVLDADALNILAGTLESLEVAKAPLILTPHPGEMARLLRTSTARVQENRLSIAHDFATRHGVVLVLKGARTLIATPEGEVFVNPTGNAGMASGGTGDVLAGMIGGFLVQGHPPRTAALLGVYLHGLAGDLAAEALGERSFIASDLLDRLPTAIAQTASHATNAFPLPRRARDAHKGNFGHVLVIGGDVGFAGAPLLAGEAAARVGCGLVTLATRPQHAGMQVAARPELMAWGVDRPAQLDPLLDRASVVAIGPGLGQSPWAAGLLARVLETNLPLVVDADALTLLARDPVQNERWVLTPHPGEAARLLDCSTAEIQGDRFTAVRELQSRYGGAIVLKGCGSLVATRKSGVTVCTDGNPGLASGGTGDVLTGVIAGLMAQGLEPESAALTGACLHGAAADRAVLEGERGMLAGDLMPWLRRLVNPV"
  },
  {
    "reference": "MDNSHVALVSMLLKAEGFSPYRCDRNIALGINLVSLTKVLRAAQNEDILTLKADDSPDAVNLMFESAETDRISEYDIKLMDIDQEHLAIPETEYAATVEMPSAEFQRICRDLNALSESVVIEATKEGVKFSCQGDIGSGSVTIRQHTSVDKPEQNVSIALSEPVALTFSLKYLVNFCKATSLSSKVTLCLSQEVPLLVEYGLGSGHLRFYLAPKIGDEE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should be able to enhance the efficiency of leading strand elongation.2. The protein contains novel Proliferating cell nuclear antigen PCNA C-terminal domains that confer a unique function or activity.3. For general function, the protein need meet that This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. The designed protein sequence is ",
    "response#1": "IWFACGPYTATDNCGYEHLCELLDKVVAEKPDILMLAGPFVDKKNTFLNKPTFNITYDNLLEDLLLKVKETLVGTKTQVIIQPNASRDLCVPPVFPSAPFQQNRKLDKIKKELIFVADPCIFRISGVEVAMTSSEPIQALSNTEFHRSANQENIDRVARLSSHLLTQQCMYPLEPTEVPASMGDLLDVCCIGSTPHIVFAPTKLAPSGIPDSEYQAIVRMPSSEFMRICKDLSSIGDTVVISVTKEGVKFSTSGEIGSANIVCRQNQTIDKPEEATIIEMQEPVSLTFALRYMNSFTKASSLSEQVTISLSSELPVVVEYKIAEMGYIRFYLAPS",
    "response#2": "IWFACGPYTATDNCGYEHLCELLDKVVAEKPDILMLAGPFVDKKNTFLNKPTFNITYDNLLEDLLLKVKETLVGTKTQVIIQPNASRDLCVPPVFPSAPFQQNRKLDKIKKELIFVADPCIFRISGVEVAMTSSEPIQALSNTEFHRSANQENIDRVARLSSHLLTQQCMYPLEPTEVPASMGDLLDVCCIGSTPHIVFAPTKLAPSENNLHNGCPAMLVGIPDSEYQAIVRMPSSEFMRICKDLSSIGDTVVISVTKEGVKFSTSGEIGSANIVCRQNQTIDKPEEATIIEMQEPVSLTFALRYMNSFTKASSLSEQVTISLSSELPVVVEYKIAEMGYIRFYLAPR",
    "response#3": "IWFACGPYTATDNCGYEHLCELLDKVVAEKPDILMLAGPFVDKKNTFLNKPTFNITYDNLLEDLLLKVKETLVGTKTQVIIQPNASRDLCVPPVFPSAPFQQNRKLDKIKKELIFVADPCIFRISGVEVAMTSSEPIQALSNTEFHRSANQENIDRVARLSSHLLTQQCMYPLEPTEVPASMGDLLDVCCIGSTPHIVFAPTKLAPSIWFACGPYTATDNCGYEHLCELLDKVVAEKPDILMLAGPFVDKKNTFLNKPTFNITYDNLLEDLLLKVKETLVGTKTQVIIQPNASRDLCVPPVFPSAPFQQNRKLDKIKKELIFVADPCIFRISGVEVAMTSSEPIQALSNTEFHRSANQENIDRVARLSSHLLTQQCMYPLEPTEVPASMGDLLDVCCIGSTPHIVFAPTKLAPSHHIHTQIDGVRIWFACGPYTATDNCGYEHLCELLDKVVAEKPDILMLAGPFVDKKNTFLNKPTFNITYDNLLEDLLLKVKETLVGTKTQVIIQPNASRDLCVPPVFPSAPFQQNRKLDKIKKELIFVADPCIFRISGVEVAMTSSEPIQALSNTEFHRSANQENIDRVARLSSHLLTQQCMYPLEPTEVPASMGDLLDVCCIGSTPHIVFAPTKLAPSHNIHTQIDGVRYTNTSSFSRPEEIETMVTEALGGDFAAARATLDDLLTNRGLAGGDIIDQVHRSVWEFDVEEAAAVRLLDRLGEADYRIAEGANERVQLEALLA"
  },
  {
    "reference": "MEAETSTQEVVKQVKNLPILMEDAPVGSDDHQLQYSYTFSYFMRPTGKFDPEDYASYVQPVGIMKSVEQFWSIMVHFKRPTEMCDKADIHFFKTGVKPVWEDPANCKGGKWIIRLKKGLSTRIWENLLMAIIGEQFLVGDELCGAVCSIRNQEDIISLWNRNADDTPVTNRIRETLRSVLQLPQNTVLEYKRHDDCLRDQSSYRHTTKNICK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be optimized for eukaryotic translation initiation factor 4F complex-specific folding and stability, while maintaining translation initiation factor activity, RNA 7-methylguanosine cap binding. The designed protein sequence is ",
    "response#1": "MQLIEKALDSRRREATLESGLEQLETSTAQMIEWLERMLHYVEDVNKNGEKPGDAQIGRQLMDIVTASSNNMQPEKLDTLVKNTLRDYVMVSYLAKLTQTQLQVHERI",
    "response#2": "MTKAASFVLLSALAIPVISTSRSRGALIDVDEVRKNKNTRLTNLLGMLQSPTRAVLFRDALNKGLLKRVRPEIRELYNILEVDFHPLSICKKVTPILKKIGDDPEMEKYVVPLQQVILTRLFQQLSQVYESVELKFVYELAQFPDPFQITPSMIEKFIMNGCKKGDLAIRVDHISGVLTFDTDVL",
    "response#3": "MTKAASFVLLSALAIPVISTSRSRGALIDVDEVRKNKNTRLTNLLGMLQSPTRAVLFRDALNKGLLKRVRPEIRELYNILEVDFHPLSICKKVTPILKKIGDDPEMEKYVVPLQQVILTRLFQQLSQVYESVELKFVYELAQFPDPFQITPSMIEKFIMNGCKKGDLAIRVDHISGVLTFDTDVL"
  },
  {
    "reference": "MRIIFWGTPEYSISSLDIFIKSKHEVIAVVSQPDKKRSRGKKLISSPVKSFAEQESIKIYTPEKIRDNINFINELKSLSCDLFIVIAYGKILPKEILEIPKFGCWNAHASLLPRWRGAAPIQWSLMKGDEFTGVGIMKMNEGLDTGDLLLEEKIKIDNNDNLITLTEKLSILSAKLFLNATSLLEENINKNTNYQLTKQNTLGREITYARMIEKSDYKVDWGNEAIKISRKIKALYPRANTTFRGKNLKIIKIKVLSSDEINNEKYCLMSNYSKPGIILAVLENEGIIISTKTDPIILLEAKLEGKNISSKKQLIQQLKPSLGEYLSN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The (6S)-5,6,7,8-tetrahydrofolate binding site should be stable and able to withstand changes in environmental conditions.2. For general function, the protein need meet that Attaches a formyl group to the free amino group of methionyl- tRNA(fMet); The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus.3. The protein must have high specificity towards methionyl-tRNA formyltransferase activity.4. The protein have enhanced (6S)-10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) =   (6S)-5,6,7,8-tetrahydrofolate + H(+) + N-formyl-L-methionyl-   tRNA(fMet). The designed protein sequence is ",
    "response#1": "MKLVFAGTPEVAVPALDALIASDRHEVAAVVTRPDAPAGRGRRLVASPVAERAQEAGIEVLKPAKPRDEEFLARLREIAPDCCPVVAYGALLPKVALDVPARGWVNLHFSLLPAWRGAAPVQHAVMAGDEVTGASTFLIEEGLDSGPVYGVLTEEVRPTDTSGDLLTRLAFAGAGLLGKVESRLDWEQPAERLACRVRAFQPWPVAEVVLSGERVRIHEALALDLDHSQPPGTVLAASKEGIDVACVQGALRLCRLQREGGKAITVADYLNALGKVESRLDWEQPAERLACRVRAFQPWPVAEVVLSGERVRIHEALALDLDHSQPPGTVLAASKEGIDVACVQGALRLCRLQREGGKAITVADYLNAESGFIDFNLSAFEIKNKINACNPWPLVRVRLDYNDIIFHRADFLEVDLYKERKIGEIVDFNPEKGLFVNTGKGILLLLEVQRPGRKVLLDELGLQASCI",
    "response#2": "MRVVFAGTPEPAVPSLRRLIESANHEVVAVVTRPDAVAGRGRKVTRSPIGLLADEHGIPVLTPVKASDPDFAAELARLEPDCAPVVAYGNLLPQNVLDIPKYGWVNLHFSLLPAWRGAAPVQAAISAGDEVTGASAFRLEAGMDTGPVYGVMTERIRDTDTAGDLLGRLAENGAALLESLVAYFRRYEHEKLDWTMSNQEVFNHIRGMNPWPVAHTFLEGQRLKIYEAQLAEGEGLPGQVVVKTKKSLVIATGQGALSLIVVQPAGKPKMSAADTLFSH",
    "response#3": "MRIIFMGTPEFACPTLQKLLDRGEDVIAVITQPDRPKGRGQQTLPPPVKVLAERHGIPVMQPLKVRVPEVVESIRSLAPDLIVVVAFGQILPKSLLDIPKYGCINVHASLLPRWRGAAPLNWCIINGETETGVTTMMMDVGLDTGDMLVKRSTPIDPDENTQSLHDRLSVVGAEALAEKLSKEEARLDWQLPAEQLARCVRAFNPWPVSFFMLEGQPVKVWGANVLHQESDAQPGTIVGADKQGIAVATAQGILLLTQLQPAGKKSMAARDLLNSEDAQVQWSAPALRVDRVVRGCTPAPGAWTVFRGERLKLIQATPVLDRTDLAPGELSAAKNNVYVGTGSHAVELLWVQPQGKKPMRLRDGLEQLQRRFSIDL"
  },
  {
    "reference": "MSSTLLEQTRSNHEEVERLERLVVEDLQKEPPSSKDRLVQGHRVRHMIESIMLTTEKLVETYEDKDGAWDDEIAALGGQTATGTNVFSEFYDRLKEIREYHKRHPSGRLVDANEDYEARLKEEPIIAFSGEEGNGRYLDLHDMYNQYINSKFGERVEYSAYLDVFSQPEKIPRKLKLSRQYMKYMEALLEYLVYFFQRTEPLQDLDRILSKVCSDFEEQYADGIVEGLDNELIPSQHTVIDLDYYSTVEELVDVGPEKLKEALGALGLKVGGTPQQRAERLFLTKHTPLEKLDKKHFARPPHNGKQNGDAKSTHESENAKEIALTEAKVKKLCNLLDETIERTKQNIVKKQSLTYEEMEGEREGEEANTELESDDEDGLIYNPLKLPIGWDGKPIPYWLYKLHGLGQEFKCEICGNYSYWGRRAFERHFKEWRHQHGMRCLGIPNTKNFNEITSIEEAKELWKRIQERQGVNKWRPELEEEYEDREGNIYNKKTYSDLQRQGLI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should emphasize Acidic residues compositional bias.2. The designed protein should have a specific function within the spliceosomal complex.3. For general function, the protein need meet that Splicing factor homolog to SF3a60 that may be involved in pre-spliceosome formation; Is necessary for gametic cell fate determination. The designed protein sequence is ",
    "response#1": "VKSGTQRSVKVIEHKKDPLQPVTHRKRKVVVPSEEIQAPILHKSDDPSIKPTKEELDKWRIPSAISNWKNPNGFAISLDNRVAIESIKTDCPDNDKKDNFLLLSEALDEAEREARQRINIKQEAYKELEKEETLKKEQRLRHLAERARQDS",
    "response#2": "IGRPGYKVSKIREAESGKFGLRFQIKYPDIEVNAKPRYRIMSAYEQRVEAPDRKFQYLVVAAEPYESIAFKIDRAPGKFWSYWDAPQ",
    "response#3": "VKSGTQRSVKVIEHKKDPLQPVTHRKRKVVVPSEEIQAPILHKSDDPSIKPTKEELDKWRIPSAISNWKNPNGFAISLDNRVAIESIKTDCPDNDKKDNFLLLSEALDEAEREARQRINIKQEAYKELEKEETLKKEQRLRHLAERARQDA"
  },
  {
    "reference": "MARVVKVFRTLRNNWKKSTFAVCVLSYGGYWLYGKHSQGCGTDCSDNVLRREACLAAREFGRQQIAPQERLRKATVILNPAACSGKANKLFEKNAAPLLHLAGVEITIVKTDYEGQAKKLMELMEQTDMLIVAGGDGTLQEVITGLLRRADQVVCNIPIGLIPLGSHNSLSPSLHLLSDNKVKDITSATLSILKGETVPLDVLQIKGEKEQPVFALTGLRWGAFRDVAATISKYWYLGPLKTNAAHWFSTLREWPLVREISVSYLAPSLRPADLHPQKPPRPNLLSRIIRRLQNRWSPPIEEPPKVEEPEQWKEEHLSTLELFIQTQNKNPMERRINDSLMICAEPDNFTVGEYITVGSKKEEDPSAFTKDSTKLEASACQLQLPEGASGFYNIDNEEYEAMPVEVRLLPRKLRFFISAERREQLLSLAQG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must be able to perform kinase activity under specific conditions. The designed protein sequence is ",
    "response#1": "PILLEDVKQQNLSQLMVVSPDVGGVVRARAMAKRLNDAELSIIDKRRTGPNKSEVMHIIGEPAGRNCLIIDDIVDTAGTLCSAAQQLKRHGAMSVRAYITHPVLSGPALNNIIHSELDEVVVTDK",
    "response#2": "YRLGRKIGSGSFGDIYLGTNISTGDEVAIKLESVRSRHPQLIYESKLYKILTGGIGIPTLYWYGIEGDYNVMIIELLGPSLEDLFSICNRKLSLKTVLMLADQMLNRIEFVHSRHFIHRDIKPDNFLIGRGKKMSIVFAIDFGLAKKYRDPRTQSHIPYREGKNLTGTARYASVNTHLGIEQSRRDDLEALGYVLMYFNRGSLPWQGLKATTKKDKYDKIMEKKMSTPIEVLCKQFPFEFITYLNYCRSLRFEDRPDYSYLRRLFKDLFR",
    "response#3": "YRLGRKIGSGSFGDIYLGTNISTGDEVAIKLESVRSRHPQLIYESKLYKILTGGIGIPTLYWYGIEGDYNVMIIELLGPSLEDLFSICNRKLSLKTVLMLADQMLNRIEFVHSRHFIHRDIKPDNFLIGRGKKMSIVFAIDFGLAKKYRDPRTQSHIPYREGKNLTGTARYASVNTHLGIEQSRRDDLEALGYVLMYFNRGSLPWQGLKATTKKDKYDKIMEKKMSTPIEVLCKQFPFEFITYLNYCRSLRFEDRPDYSYLRRLFKDLFR"
  },
  {
    "reference": "MKGPLLAKLEAIRTPTILVIGDLMLDLYVWGSVDRISPEAPVPVLKVESEEPRPGGAGNVAVNVAALGANVFCCGVVGADAYGRSLCGMLKKLKVDVSGVLHDRARPTILKTRMMAHSQQLMRVDKEECHDLGKSIQSRMLDYITEILPRCDAVLVSDYGKGTLPDEVIAQVIAASRKRGVKVLVDPARQRDYRVYAGCTVLKPNSAEAAQASGMQIVDTNSLAAAASKLRKLSSCEYLVVTQGSEGMTIFRSGAAPVHIASLSRPVFDITGAGDTVLSLLGYVLAGGHTIDAAAEIANVAASIVVGRVGAVPVTKAEITRELAGFHHIASHKVRSLEEMISTCEEHRRRKQKIVFTNGCFDLLHVGHIKLLQFAKNSGEIVVVGLNSDSSVRRLKGQGRPVLEQNERAYILSALEQVDYVVIFDEETPLKLIKAIRPDILVKGADYTKDTVVGRDFVESYGGKVRLAPLIEGVSSSGIISRVVRNSE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have at least one Carbohydrate kinase PfkB domain.2. The protein with the reaction ATP + D-glycero-beta-D-manno-heptose 7-phosphate = ADP + D-   glycero-beta-D-manno-heptose 1,7-bisphosphate + H(+), the active site must be optimized to ensure efficient catalysis.3. The designed protein must be able to perform phosphotransferase activity, alcohol group as acceptor, heptose 7-phosphate kinase activity, ATP binding under specific conditions.4. The designed protein should interact with enzymes and substrates within the ADP-L-glycero-beta-D-manno-heptose from D- glycero-beta-D-manno-heptose 7-phosphate: step 3/4, Nucleotide-sugar biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MPSFQQAKLLVVGDVMLDRYWHGAASRVSPEAPVAVVRVENKEDRPGGAGNVALNIAALGSAATLVGLVGKDDTGEELASRLKAAGVYCEFLQSEDKPTITKLRVISQHQQLIRLDFEQKFDKKDVDGLVGKIAGLIDSAQVLVLSDYDKGALQDIEEIIALGRARNIPIVVDPKGSNFEKYRGATLITPNLIEFEEVVGHCANEDELLARGLKLLTDLDLQALLITRGEYGMTLIRPDSAELHFPARAQEVFDVTGAGDTVISVLAAALAAGENLADSTAIANLAGGLVVGKLGTAAISGPEMRRALADADSRAHRVTDLRARGERVVMTNGCFDLLHPGHIAYLEAARALGDWLIVAVNDDASVRRLKGASRPLNDLEHRMQVLRGLAAVDWVVPFAEDTPERVICRLTPDVLVKGGDYAPDEIAGGDCVRAAGGEVRVLDFVDGYSTTAIIERMRG",
    "response#2": "MPSFQQAKLLVVGDVMLDRYWHGAASRVSPEAPVAVVRVENKEDRPGGAGNVALNIAALGSAATLVGLVGKDDTGEELASRLKAAGVYCEFLQSEDKPTITKLRVISQHQQLIRLDFEQKFDKKDVDGLVGKIAGLIDSAQVLVLSDYDKGALQDIEEIIALGRARNIPIVVDPKGSNFEKYRGATLITPNLIEFEEVVGHCANEDELLARGLKLLTDLDLQALLITRGEYGMTLIRPDSAELHFPARAQEVFDVTGAGDTVISVLAAALAAGENLADSTAIANLAGGLVVGKLGTAAISGPEMRRAAMSGRTVLCVGDLMRDEFVYGEVARISPEAPAPVIAATRSERHIGGAGNVARNIAALGARCIFAGVVGDDEAGRALRAELAAVPGIEAHLVTDASRPTTRKVRFVSDHFSTHMLRADWEIATPVDAAVENELIGIVEAALPRADIVLLSDYAKGVLTVGVIRATIDGARRLGKRVVVDPKNADFTVYRGASVLTPNQREFAAASRRRATTTPEIVAAAREVMGEGAFEAMLVTRSEHGMTLVPREGEAVHVPAHPVRVRDVSGAGDTVVGVLAVALGAGAGWESALRLANAAAAVAVGKQGTATVSAAELRRSIARGDHAAQFGIVTERQLAAAIAEWRAAGETIVFTNGCFDLLHVGHITLLEDCRRFGSKLVLGLNSDDSIRRLKGPSRPIVGDRERSRVMAALAAVDAVVLFEEDTPLELIRAVKPDVLVKGGDYIAESVVGYEDVTARGGRVEIVPTVEGFSTTNIVRKL",
    "response#3": "MPSFQQAKLLVVGDVMLDRYWHGAASRVSPEAPVAVVRVENKEDRPGGAGNVALNIAALGSAATLVGLVGKDDTGEELASRLKAAGVYCEFLQSEDKPTITKLRVISQHQQLIRLDFEQKFDKKDVDGLVGKIAGLIDSAQVLVLSDYDKGALQDIEEIIALGRARNIPIVVDPKGSNFEKYRGATLITPNLIEFEEVVGHCANEDELLARGLKLLTDLDLQALLITRGEYGMTLIRPDSAELHFPARAQEVFDVTGAGDTVISVLAAALAAGENLADSTAIANLAGGLVVGKLGTAAISGPEMRRALADWRRQGLRIGFTNGCFDILHPGHVRVVTQARAACDRLVVGLNSDASVRRLKGADRPVQTERARAEVLAALEAVDLVVIFDEDTPLELIEAIRPGVLVKGSDYRPEQVVGHDVVTSYGGQVVLVDLLQGFSTTALVERAKEG"
  },
  {
    "reference": "MSGILTRWRQLGRRYFWPHLLLGMVAASFGLPALSNAAETNTPARTTASTASKVNFSHLALLEASNRRPNFTVDYWHQHAIRTVIRHLSFAMAPQTLPVADAPSPLQAHHIALLNTLSAMLTQEGTPPAIVRRLSLAYFAPQTAFSIPAWISQAQGIRAGPQRLS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Translational pausing at a specific Pro residue 5 residues before the end of the protein may allow disruption of a mRNA repressor helix that normally suppresses secA translation initiation.; Regulates secA expression by translational coupling of the secM secA operon.2. The protein can be expressed in periplasmic space and perform translation regulator activity without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "ECKAGFAGDDAPRAVFPSIVGRPRH",
    "response#2": "EHRVP",
    "response#3": "MCPG"
  },
  {
    "reference": "MRRMDRWTVCRRLASMARPVSFLEAALMAVVAACIVLGKWFDVMSIKRRGLIINGLKDIKGMGSANVSSIYGDAAAFLVLRTLSSAFTESKAILFSAVTNRVVQASTSRILDLTMHAAHSCEIKPTELNRIVERGNRKISKVLVKTLTVATPALFRLALLFREVHAMFGPKYLVPILFTAAAYAAYTCVMLRIRARYRKEINNADNSVSRRIHECVSNVDLVRACCSEQFEVSRLAGEMETMWALKLSDKGCVGMTNLGQRALFSVLFVHVAFKGIADMAALRMTVGDLSVLFSFVLSIDASMWTLGGIARDLGFWLTDCTDLLCLHDGLERAAEQGPGAGAAAEGMAACPSSPPGEAAAVEFDDVSFAYPRSAAYPRSAHVLSGVSFRIMRGERVGIIGRPGSGKSTILRLILMLHRHKGRIRVNGAELWSASPRAVRGSIGCILQDGLLFDESILYNVMYGCPRAGFHRVLRECKNAGLSDVVRRKGLHSRMKALSGGEMQMVSLARCFLKDAPLMLLDEATSKLDAETERDVFGLMMGMRGKTIVMVLHDLWMTEHLDRVILVDSGTVKEVGTHSELMGLRGMYWRMKTASRE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of ABC transmembrane type-1 domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "QGDSVAVVGASGSGKSTLLHLLGTLDNPSRGTVLFRGRDITAMTAEQKAAMRSRDVGFVFQFHHLLPEFNTQENVAMQAVIAGMPRREAMKKAAHALELVGMSGRAGHRVGTLSGGERQRAAIARAILMHPAVLLADEPTGNLDERTGDSVGQLLMDLNRELGMTLVVVTHNNSLASLMNRRLELRAGR",
    "response#2": "LERALNVLVPIFYRNIVNLLTEKAPWNSLAWTVTSYVFLKFLQGGGTGSTGFVSNLRTFLWIRVQQFTSRRVELLIFSHLHELSLRWHLGRRTGEVLRIADRGTSSVTGLLSYLVFNVIPTLADIIIGIIYFSMFFNAWFGLIVFLCMSLYLTLTIVVTEWRTKFRRAMNTQENATRARAVDSLLNFETVKYYNAESYEVERYREAIIKYQGLEWKSSASLVLLNQTQNLVIGLGLLAGSLLCAYFVSEQKLQVGDYVLFGTYIIQLYMPLNMLGSVYNSLKNS",
    "response#3": "LILVMLMVVISAIFGLLGPFVIGKAIDHFIVGKTVSGLIPVLLLLLAIYIIQSLSLWFQNYWMITISQGTVFRMRSELFTHLHELPIPFFDKQRHGELMSRVTNDIENVSSTLNTSVIQILSSVITFVGTIAVMLYMSPLLTLITLTIIPVMAASLKWITNRTGKLFKEQQKNLGDLNGYIEESVSGAKVIKAYSREKQITAEFLEKNAALKTSGFWAQTISGFIPKVMNSLNNLSFTMIAAIGGLFALKGWISIGSIVVFAEYSRQFTRPS"
  },
  {
    "reference": "MLIGYRGTGKSSIGKALSRMTGQGYLDTDQLIEDVTGKKISQIFESEGEAGFREIEQHVIARIPSDAGIISTGGGAVIRPVNVRMLRMNSLVILLNTSPEVIFNRIHKSDRPSLTGLSPREEIEQVLKERMPIYRSVADIVIDTTITTPEEAAKQILNLIENGICVPDKRKELFASVMKTAIPSGEKIHLKKISGDYDIFLYGILGYPCMHSKSPGIYNHLFADYGMPAHYTWFEHKDPGRFLSLLPGTGVRGLSVTIPHKETVIPYLDEIKHDAESIGAVNTILIQDDKKYGFNTDWKGIYRPLEGTHGDTAVILGAGGAAAAAVYAVSMRGFTPVILNRTPERAEHLARKSGAEIGTLADIGKYHPDLLINATPIGMGSDPHTPIPPSALEPGMKVFDLVYTPRDTPLLQAALASGCSVIPGTEMFIHQLAEQFRILTGIETPISRLREILA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be optimized for shikimate kinase activity activity in order to improve phosphorylation, aromatic amino acid family biosynthetic process.2. The designed protein should have a binding site that is specific to the target NADP(+), shikimate ligand.3. The protein must exhibit the following characteristics: Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids; Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA)..4. The protein contains novel Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase, Shikimate dehydrogenase substrate binding N-terminal domains that confer a unique function or activity.5. A protein that can modulate the overall flux of metabolites through the chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7 pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "LTVAPGEESKSFETWQRLVTSLLEQKVDRHTTIIALGGGVVGDLAGFTAAATLRGLPFIQIPTTLLSQVDSSVGGKTGINTPQGKNLAGAFHQPLLVLADTGALKTLPVRELTAACSAISQRKFANLCRRTVVVLGAGGAAKAVVYMCLLDGAEKVYLLNRTVEKAQAIAVQMNGLGKDTMVIPKAMKDYATISEENLIVFQATSVGMYPNVEATPIDNQVLDSSQVVYDMVYNPLETRLLREAKTRGAKTISGTDMLVYQGVAQLEWWLSRPIPEFPCVAAMKQALLSL",
    "response#2": "MSEIVIPAGEEHKTIQTVAAIWEGLLSAGVERGSVVIALGGGVTGDLTGFAAATYLRGVPWVNVPTSLLAMVDSSLGGKTGADLPQGKNLIGAFHAPLLVLSDPEVLSTLPPAEALSGKKILLVGAGGAVKGVMAPLLDEGPASIVVANRTVSKAEDLARIFGRNGVVCARAFADLEGPFDVIINGTSAGLEGELPGDLSIPQAALDRGVDMFVRQAAYQYKLFTGNEAPIKLMRKTFRLLRE",
    "response#3": "MLTVAPGEESKSFETWQRLVTSLLEQKVDRHTTIIALGGGVVGDLAGFTAAATLRGLPFIQIPTTLLSQVDSSVGGKTGINTPQGKNLAGAFHQPLLVLADTGALKTLPVRELTAXSDRILGHQRFLVLGAGGASRAVLLALQQAGVNSLWVTNRNEIKGQELAQKFGAAFLPPAEIPRKIASFDLVISCLPRTEYLLPSVSSWQGKTAAILGAGGAARGIALALKEKGVRLIILNRTPGKAEELVRLLRAGGGEACAEELAAGAWLSEADLVVQTSVSSWQGKTAAILGAGGAARGIALALKEKGVRLIILNRTPGKAEELVRLLRAGGGEACAEELAAGAWLSEADLVVQTTGLLGHQRFLVLGAGGASRAVLLALQQAGVNSLWVTNRNEIKGQELAQKFGAAFLPPAEIPRKIASFDLVISCLPRTEYLLPH"
  },
  {
    "reference": "MSVVILAAGQGKRMVSRLPKVLHRVGGTTLIDRVITTARRLKSATICVVYGHGGELVRQSIGARDVVFVRQAPQLGTGDALRRALPMIGESDVTLVLYGDVPLVRAETLAPMVAGKIERVMVLTAMPDDPSGYGRVLRDRRGRIKRIIEDADATPVQRRIREINTGIMALPTKRLASWLGRLETRNAQGEYYLTDVLAQAGADGVPVEAATAADPAEVLGVNSRDHLAQLERTYQRRYAQNLLERGVSLADPARIDVRGELECGDDVAIDINCVFLGAVSLGADVSIGPNCVLKNVAIGDRTVVEPYTLIEDSVIGADCRIGPYARIRPGARLARDVHIGNFVEVKNSTIGEHSKANHLAYIGDAAVGARVNIGAGTITCNYDGANKHRTVIEDDVQIGSDVQLVAPVTVGKGATVGAGTTVWKRAPAGALTINEKTQVTRPGWKRPAKKQR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein should be able to manipulate the activity of enzymes in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway, either by activating or inhibiting their function.2. The protein design must be able to perform glucosamine-1-phosphate N-acetyltransferase activity in order to contribute to cell wall organization, cell morphogenesis.3. The designed protein should be able to compete with endogenous proteins or inhibitors for UDP-N-acetyl-alpha-D-glucosamine binding. The designed protein sequence is ",
    "response#1": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLTVEVDDPTGYGRIVREEGRIVGIVEERDASAEQRAIKEVNTGILIANGADLKRWLSRLTNDNAQGEYYLTDVIAMAHEDGMAVHPVQVAASHLAAGVNNRLQLAELERVYQREQAERLMREGVTIVDPTSVWVDVGVDLAPDCEILPGVQLHGATSVATGARIGPDTTLTDTRVGAGAEVVRSHVLDSRIEAGARVGPYAYLRQRVVVGPGAKIGTFVEAKNTRLDDGAKIPHLSYMGDATIGAGANVGAGSITANYDGVHKHPTVVGPQARAGSNTMFVAPVELGAGAYTGAGTTLREDVPAGALAVSTPPKRVKNWQRPRK",
    "response#2": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVTVLDPTGYGRIVRDAGGDVLRIVEQKDATDSVVENDVNVGPFAYVRPQSHIGAGAKIGDFVELPERLWQERRRHELMLSGVTMIAPETVFLSHDTQIGQDALIEPNVVFGPGVVVEGGAVIHAFSHLEGAYVATGATVGPYARLRPGANLAEGSKVGNFCEVKKAEIGKGAKVNHLTYIGDAFVGAESNIGAGTITCNYDGVNKHETRIGERAFIGSNSSLVAPVTIGDGALVASGSVITKDVPADALAFGRSRQENKAGLAERLRARRG",
    "response#3": "AVVLAAGKGTRMKSSLHKVLHQVLGKSMVDHAVTNLEKIGVDKIVTVIGYEAESVRAELQDRVEYAMQTEQLGTGHAVMMTKELLEGLDGVTIVTYGDVPLLTEQTISNLFDYHQSQKAAITILTACTDNPTGYGRIIRDEEGNVLRIVEQKDANTEELLVKEINTGVCCYDNKVLFEALTKITNNNSQGEYYLTDLVGIIRDMGLKVVAYVNEDFEETLGVNDRVALAEAERIMRERINTYHMRNLAEAEAVLQARLRKAAMEAGVTLIAPETVYLAADTKFGRDVTIEPFVVIGPGVTIGDGAVIHSFSHIVEATIGKKVSIGPYARLRPGTSLGDGSRIGNFVETKAAILEEGVKVNHLSYVGDAHVGANSNLGAGTITCNYDGFLKHKTLIGKGAFIGTNSSLVAPVKIGAGAYIGSGSVITRDVPDDAMAVERSPQKNIEGWVQKKGW"
  },
  {
    "reference": "MTGAFNTGRTVALVVAAGSGTRAGGDVPKQYVPIAGRPMLAHALAALEHPAIDAVHVVIAPGQEAQFAGISDIVPILGGDTRQQSVRNGLEHLAAESGIDRIFIHDAARPFLPPVVIDRLLAALESADGAVPVLPVVDTLANGGDALGDVADRSALVRVQTPQAFRFDAILAAHRAWDGAANATDDAQIARAAGIRVALVEGDAMLDKLTYAQDFARAEERLGARLVSRTGLGFDVHALADGEELWLGGIRIPHDRGLKGHSDADVLLHAITDALLGALADGDIGSHFPPSDPQWRGAASGQFLEHARDLSIARGGRIDFVDATVICEAPKIGPQREALRHSIAALLRLPVSKVSIKATTTERLGFTGRGEGIAAQAAVSIRMPEGDQS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein with 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity, metal ion binding, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity functions is required to enable terpenoid biosynthetic process.2. The presence of a divalent metal cation is necessary for the protein to function in the desired environment.3. The designed protein should have a high specificity for a divalent metal cation, 4-CDP-2-C-methyl-D-erythritol 2-phosphate binding, with minimal off-target effects.4. The designed protein have 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domains with specific sequence motifs that are crucial for its activity.5. The designed protein should interact with enzymes and substrates within the isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6, isopentenyl diphosphate biosynthesis via DXP pathway, Isoprenoid biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MIRATRTGIGFDVHRLVEGEELWLCGVKVPHGKGLSGHSDADVAIHALVDALLGAVALGDIGDHFPPSDPQWKGASSDRFLAHAAHLARDAGYDIGNVDVTIICEAPKIGPHKTAMRKALARILGIDLDAVSVKATTTERLGLTGRGEGIAAQAVATVTSRAVAAGRTDDAAVAERAGLKVVMVEGSEDNRKITTAEDLARTVAMETRTALGFDVHGFGPGNAVMLGGVAIPHTHKLAGHSDADVALHALTDAVLGTIGAGDIGQHFPPSDPQWRGVSSDRFLQHAVGLLGARGGRIVHLDLTLVCEAPRIGPHREAMAGSIARIAGIDVGRVSVKATTTEGLGFTGRREGIAAQAVATVTSRAVAAGRTDDAAVAERAGLKVVMVEGSEDNRKITTAEDLARTVAMETRTALGFDVHGFGPGNAVMLGGVAIPHTHKLAGHSDADVALHALTDAVLGTIGAGDIGQHFPPSDPQWRGVSSDRFLQHAVGLLGARGGRIVHLDLTLVCEAPRIGPHREAMAGSIARIAGIDVGRVSVKATTTEGLGFTGRREGIAAQAVATVTSRAVAAGR",
    "response#2": "KIGHGFDAHRLIEGRKLILGGVEIPYRLGLDGHSDADVLIHALIDALLGALGAGDIGRHFPDTDATYKNIDSRRLLEKTMALAKDNNMHLGNADLTLVCQQPKLVNFLEKMRKNLAQACQTQLKNINIKATTTEKMGYTGRGEGICAHAVVIL",
    "response#3": "MIRATRTGIGFDVHRLVEGEELWLCGVKVPHGKGLSGHSDADVAIHALVDALLGAVALGDIGDHFPPSDPQWKGASSDRFLAHAAHLARDAGYDIGNVDVTIICEAPKIGPHKTAMRKALARILGIDLDAVSVKATTTERLGLTGRGEGIAAQAVATVS"
  },
  {
    "reference": "MWTFAIVLAFLLIGLDEGEALDGYPLSKNNYCKIYCPNTEVCKDTCKRRAGATDGECRWDGCYCFNVAPDTKMYPGELPCH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be optimized for sodium channel inhibitor activity.2. The protein must exhibit the following characteristics: This toxin binds, in vitro, to sodium channels and inhibits the inactivation of the activated channels.3. The presence of LCN-type CS-alpha/beta domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "LDGYPLSKNNYCKIYCPDEKVCKWSCKHRAGATNGKGDCINKGCYCYDVAPGTEMYPGRLPCS",
    "response#2": "KEGYPVGRDGCKISCVINNNFCKVECQAKWRQSDGYCYFWGLSCYCTNLPEDAQVWDSSTNKCK",
    "response#3": "KEGYPVGRDGCKISCVINNNFCKVECQAKWRQSDGYCYFWGLSCYCTNLPEDAQVWDSSTNKCR"
  },
  {
    "reference": "MGAQVSTQKSGSHENTNFAAGGSTINYTTINYYKDSYAASANKQDFSQDPSKFTQPVVDALKEGAPVLKSPSAEACGYSDRVAQLTVGNSTITTQEAANITVGYGVWPEYSKDTEATAVDKPTRPDVSVNRFYTLGAKLWQTDSKGWYWKFPDVLTETGVFGQNAQYHYLYRSGFCIHVQCNASKFHQGTLLVAAIPEYVVARASNETKPNTAPHPNYNVTQPGPDGKELAFPYLLDNGTALSQALIYPHQWINLRTNNCATIVMPYINCLPFDSALNHCNFGLVVIPVAPLAYSAGATTAIPITISIAPLNSEFAGLRQAVSQGLPAELKPGSNQFLTTDDGVSAPILPGFHPTPEMFIPGEVKNLLELAQVESILEVNNLNDNEANPMQRLLIPVSVQTNVDTLCAAFRTDPGRDGPWQSTLVGQLCRYYTQWSGSLEVTFMFTGSFMATGKLLIAYTPPGAPQPATRALAMLGTHIVWDFGLQSSATLVIPWISNTHFRGTSTNGVYNYYSSGIVTIWYQTNFVVPDGAPTSAYIIALAAAQPNFTMRLCKDTDQISQEAIFQGDKLEAAIENKVLSTIQSAGTHLTAANTSESTHSIGTGHTPALQAAETGATSTASDESMIETRCVVNKHSVAETSITNFYARAGLVGYLTLLTSGDTANGFTNWDIDIMAFVQQRRKLEIFTYMRFDAEFTFVATSEDGSTPNTMLQYMYVPPGAPKPTGRDTFEWQTATNPSIFVKMSDPPAQVSVPFMSAASAYQWFYDG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that The oriI viral genomic sequence may act as a template for this2. The designed protein must have at least one SF3 helicase domain.3. The presence of Mg(2+) is necessary for the protein to function in the desired environment.4. The protein should be optimized for host cell nucleus-specific folding and stability, while maintaining cysteine-type endopeptidase activity. The designed protein sequence is ",
    "response#1": "HPTKEIFIPGKVNNLSEICQVDSMIPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLVDVASQPIATTLIGEIASYYTHWTGSLRFSFMFCGTANTSLKLLIAYTPPGVPKPENRQKAMLSTHVVWDVGLQSTVSMVVPWVSASHYRYTTPDTYSQAGYITCWR",
    "response#2": "HPTKEIFIPGKVNNLSEICQVDSMIPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLVDVASQPIATTLIGEIASYYTHWTGSLRFSFMFCGTANTSLKLLIAYTPPGVPKPENRQKAMLSTHVVWDVGLQSTVSMVVPWVSASHYRYTTPDTYSQAGYITCWR",
    "response#3": "HPTKEIFIPGKVNNLSEICQVDSMIPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLVDVASQPIATTLIGEIASYYTHWTGSLRFSFMFCGTANTSLKLLIAYTPPGVPKPENRQKAMLSTHVVWDVGLQSTVSMVVPWVSASHYRYTTPDTYSQAGYITCWR"
  },
  {
    "reference": "MMKCLLIATGQHVPTWVAQGFAEYHRRLSYWLPLELVEIEPSMRGKNHDPQRAIEDEGRRVMAALPKQPYAVTLDVKGKPLNSEQLAQRMEHWRGLGRNLVFLIGGPEGHSQEVLNISNERWSLGPLTLPHMLVRLIVVEQLYRAATILTNHPYHRGK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be able to bind S-adenosyl-L-methionine ligand in a variety of conditions2. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "VGAGPGDPDLLTFRALRLMQQADVVLHDRLVPDAIIDLCRRDAERIYVGKARANHAVPQEQINQLLVRLAKEGKRVLRLKGGDPFIFGRGGEEIQELTANAVPFQVVPGITAASGCSTYAGIPLTHRDFAQSVRFITGHLKDGSTNLPWADLVAPGQTLVFYMGLVGLPEICARLIEHGRGGETPMALIQQGTTINQKVLIGTLATMPELVRNTEIRPPTLLIVGEVS",
    "response#2": "VGAGPGDPDLLTFRALRLMQQADVVLHDRLVPDAIIDLCRRDAERIYVGKARANHAVPQEQINQLLVRLAKEGKRVLRLKGGDPFIFGRGGEEIQELTANAVPFQVVPGITAASGCSTYAGIPLTHRDFAQSVRFITGHLKDGSTNLPWADLVAPGQTLVFYMGLVGLPEICARLIEHGRGGETPMALIQQGTTINQKVLIGTLATMPELVRNTEIRPPTLLIVGEVTG",
    "response#3": "VGAGPGDPDLLTFRALRLMQQADVVLHDRLVPDAIIDLCRRDAERIYVGKARANHAVPQEQINQLLVRLAKEGKRVLRLKGGDPFIFGRGGEEIQELTANAVPFQVVPGITAASGCSTYAGIPLTHRDFAQSVRFITGHLKDGSTNLPWADLVAPGQTLVFYMGLVGLPEICARLIEHGRGGETPMALIQQGTTINQKVLIGTLATMPELVRNTEIRPPTLLIVGEVAGS"
  },
  {
    "reference": "MMNDFELSLKSLGVSFTSKSIDKLRFYIEKVLLFGTRFNLVSNNDRNFDAVLLHVLDSVAGLPIIKDKNPRQVLDVGSGAGFPGIVLALFDNFRKYILLERSNKKATFLRMISLELGLDNIEVLERDVDKEQNKYEFITIRAFRDIREYARILKLILKDGGLIVAYKGKLNKIKFEVSHIESLFNKIEIKSSTMNDGKERNFLLLYK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Specifically methylates the N7 position of a guanine in 16S rRNA.2. The protein should have a high affinity for S-adenosyl-L-methionine. The designed protein sequence is ",
    "response#1": "SQIGCLMNCDFCATALMGYERNLSVSEILLQYYYVQKHLLKRGKKLGNVVYMGMGEPFSNYCAVLSSINILNSEKGQNFSKRNFTISTSGVVPVIKKFTVDESQIHLAISLHSVRDEIRNQIMPINKTWNVNKLKEALLEYQKKTKNRITFEYILIDELNCG",
    "response#2": "HKNRYTLCVSSQIGCLMNCDFCATALMGYERNLSVSEILLQYYYVQKHLLKRGKKLGNVVYMGMGEPFSNYCAVLSSINILNSEKGQNFSKRNFTISTSGVVPVIKKFTVDESQIHLAISLHSVRDEIRNQIMPINKTWNVNKLKEALLEYQKKTKNRITFEYILIDELNCEPKDARELAEFLSSFNCLVNLIPYNPVGGKIYKTPSKSKQREFYKILKDRNVNVTLH",
    "response#3": "HKNRYTLCVSSQIGCLMNCDFCATALMGYERNLSVSEILLQYYYVQKHLLKRGKKLGNVVYMGMGEPFSNYCAVLSSINILNSEKGQNFSKRNFTISTSGVVPVIKKFTVDESQIHLAISLHSVRDEIRNQIMPINKTWNVNKLKEALLEYQKKTKNRITFEYILIDELNCEPKDARELAEFLSSFNCLVNLIPYNPVGGKIYKTPSKSKQREFYKILKDRNVNVTLH"
  },
  {
    "reference": "MAKKLEITLTRSLIGRPQDQRVTVNTLGLRKMHQTVVQEDNAAIRGMVNKVSHLVTVKEIEA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have a specific function within the large ribosomal subunit. The designed protein sequence is ",
    "response#1": "GIDLIAGGKSKKTKRTEPKSDDVYLKLLVKANRYLVRRTESKFNAVILKRLFMSKVNKAPLSLSRLVRYMDGKDGKIAVIVGTVTDDVRIEDVPALTVTALRFTESARARIHKAGGECLTFDQLALPCPTWSENTVLLRGPKNTREAVKHFGPAPGVPHSHTKPYVRQTGKKIEIARGRRRSRGFKR",
    "response#2": "MEGIDLIAGGKSKKTKRTEPKSDDVYLKLLVKANRYLVRRTESKFNAVILKRLFMSKVNKAPLSLSRLVRYMDGKDGKIAVIVGTVTDDVRIEDVPALTVTALRFTESARARIHKAGGECLTFDQLALPCPTWSENTVLLRGPKNTREAVKHFGPAPGVPHSHTKPYVRQTGKKIEIARGRRRSRGFKR",
    "response#3": "GIDLIAGGKSKKTKRTEPKSDDVYLKLLVKANRYLVRRTESKFNAVILKRLFMSKVNKAPLSLSRLVRYMDGKDGKIAVIVGTVTDDVRIEDVPALTVTALRFTESARARIHKAGGECLTFDQLALPCPTWSENTVLLRGPKNTREAVKHFGPAPGVPHSHTKPYVRQTGKKIEIARGRRRSRGFKK"
  },
  {
    "reference": "MPTKYIIVTGGVLSGLGKGIAAASIGHLLSAKLKIVPIKCDGYLNVDPGTMNPFEHGEVFVLDDGAEVDMDFGHYERFLGVRCKSKWNLTMGKVFDMVRKKERRGDYLGKTVQYIPHVTDLIKEHIGQVVKEESPDLVIVEIGGTVGDIENELFLEAMRQMKKDVGRNNIVFVHLTYVPIPTGVNEQKSKPTQQSVNLLQQRGIFPDIIIGRCQELLTPEVKSKISNFCDVKPEAVITGLDVDDIYEIPIIFEREGLPENIHKKLNIYSPPDLRRWKNLVDNIRNPEKEITVAMCGKYTKLEDSYASIIESFNHCSAHLRCKINLKWVETTGLRDASRLGQIDGLVVPGGFGSRGAEGKIEAIRMARESNIPFLGLCLGLQLAVIEFARHVCGLEGANSTEMDTGTPHPVIDILPEQKTIIEKGGTMRLGAYPARLLKGSVVHSLYGTDEVSERHRHRYEVNPDYHEVLKEKGMVFSGMSPDGKLVEFIELPELKYFCATQAHPELKSRMESPAPLFFGFVKACMA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a binding site that is specific to the target UTP, CTP, Mg(2+) ligand.2. The protein contains novel Glutamine amidotransferase, CTP synthase N-terminal domains that confer a unique function or activity.3. The Nucleophile; for glutamine hydrolysis of the protein must be engineered to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).4. A protein that can modulate the overall flux of metabolites through the CTP from UDP: step 2/2, CTP biosynthesis via de novo pathway pathway, enhancing or suppressing its activity as needed.5. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.6. A protein with metal ion binding is needed to enhance glutamine metabolic process, 'de novo' CTP biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "MEARGLRVTNIKLDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTLARMTRQNNFTTGRIYEAVISKERRGEYLGATVQVIPHVTDEIKLRIQSAAEGADVAIVEIGGTVGDIESLPFIEAIRQLKIESGSQNALSVHVTLVPFIATAGEMKTKPTQHAVKEMRAIGIQPDVLLCRCDRALPRDMKEKIALFSNVPVDRVVAAMDVACIYELPLALHAEGIDEKLTVVIGLIGKYVELQDAYKSILEALIHAGAMNECKVEVRNIHSEYLTKENAKEKLQNLDAVLVAPGFGSRGIEGKIEAIRYARVNKVPFFGICLGMQMACVEFARNVLGMPKAHSTEMDPDTKDGVISMMESQKTITMMGGTMRLGSYACELRPDSTTAEIYGTANITERHRHRYEFNNEYLAAFEEAGFIPVGKNPLTDLVEVVEMKDHPFYIGVQFHPEYKSTVENPHPLFVGFIGASLDRVNIALVGKYVELKDAYFSVREALHHAGLFHNRDIHLTWIASEELEEKGGQALLRSAQGIIVPGGFGIRGIEGMIEAAKYARNNKIPYLGLCLGMQVMVIEFARHVLGTSEANSTEFEAETPYPVIDLLNDQENVEDMGGTMRLGNYPCDLLRDSHAGRAYGTTRIEERHRHRYEFNNKFRSQLQEAGMVCSGLSPDGKLVETCEIPNHPWMVSCQFHPEFGSRPGQPHPLFRDFVQAARASRAT",
    "response#2": "MEARGLRVTNIKLDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTLARMTRQNNFTTGRIYEAVISKERRGEYLGATVQVIPHVTDEIKLRIQSAAEGADVAIVEIGGTVGDIESLPFIEAIRQLKIESGSQNALSVHVTLVPFIATAGEMKTKPTQHAVKEMRAIGIQPDVLLCRCDRALPRDMKEKIALFSNVPVDRVVAAMDVACIYELPLALHAEGIDEKLTVPLADVPDSGKDDKWQRFIARHKNPVHSVRIAIVGKYIEIKDAYLSIKEALKHAGIDSGCLVDIKWVEAEELEKMDIDEVREVFSDVEGILVPGGFGTRGSEGKIKAIRYARENKIPFLGLCFGFQLAVVEFARHVLGLEEAASAEVSETPYPVIEILPEQRNVRNMGGTMRLGDAEITLEEGTRAYEIYGKSKIIERHRHRYEVNPRYIEAIEKKGMVFSGKCGNRMEIAEIPDHPFFIGTQFHPEFKSRPDNPSPVFRAFIAAGLAHQQRAGA",
    "response#3": "MEARGLRVTNIKLDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTLARMTRQNNFTTGRIYEAVISKERRGEYLGATVQVIPHVTDEIKLRIQSAAEGADVAIVEIGGTVGDIESLPFIEAIRQLKIESGSQNALSVHVTLVPFIATAGEMKTKPTQHAVKEMRAIGIQPDVLLCRCDRALPRDMKEKIALFSNVPVDRVVAAMDVACIYELPLALHAEGIDEKLTVPLGEVPELDDNKDSYKSLTEALAHGGFQHGLKVNINWVEAEALEMDGGERLLDDAHGILVPGGFGDRGSRGMMEAARIARERRIPYFGICYGFQWAAVEFARNVCGLEDADSTECREDAPHKVIYKLRDLLGVDELGGTMRLGSYPCRLEPGSLAARLYGTSLVDERHRHRYEFNVEYQQRLTDRGLSIAGRSPDGKFVEVIELPQHPWFLAVQFHPEFQSKPLRPHPLFADFVPPAAEPATLPDAYLSLVESLKHAGFSYDTDISIHWINSEILDKETIHDELSHVDGIVVPGGFGTRGIDGKIEAIQYARENNIPFLGVCLGMQLAAVEFIRNVVGLKNAHSVEIDEHTPYPIVTLIKELDQNDDIGGTLRLGSYPIKLKDGTKIQKAYNGVKRVEERHRHRFELNNEYKDQLEQNGLIISALGEDEKSIKAIELISHPWFVACQYHPEFKSRPTKAHPIIEGFLAKRAE"
  },
  {
    "reference": "MRTSQFPLNTLKEIPSDAEIVSHQLMLRAGLIRKLAAGLYTWLPLGLKVLRRVERVVREEMDRAGALEVLMPVVQPAELWQESGRWEQYGPELARLHDRHDRAFCLGPTHEEIITDLARNELKSYRQLPVNFYQIQTKFRDEIRPRFGVMRAREFVMKDAYSFHLDQESLAQTYDAMYVAYSNIFSRLGLRFRPVLADTGAIGGSHSHEFHVLADSGEDAIAFSTVSDYAANVEQAEALAPTAPRPAPTAALLKVETPGKKSIAEVSEFLGVPPHQILKTVAVMQTIVDDDGLDRDMFVTVLLRGDHELNEVKLGKVLGRFRFASEDEIERHMGCRPGYIGPLNLPAGTAVGPVYADRAVAVMSDFVCGANEAGYHFSGVNWERDLPLPQDEYLCDLRNVVEGDPSPDGLGTLRIARGIEVGHIFQLGTKYSAALNATVLNEEGRSQVMLMGCYGIGVSRVVAAAIEQNHDERGIVWPEALAPFQVALCPINMQTSARVRTTAERLYAELSAAGFEVLYDDRKVRPGVMFADMELIGIPHRIVISERGLDSGALEYKGRRDADNQSIGIDGVIEFLRERSESR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. We are looking for a protein with broad substrate specificity for ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-   tRNA(Pro).2. A protein that contributes to prolyl-tRNA aminoacylation by functioning in cytoplasm with ATP binding, proline-tRNA ligase activity.3. The protein must exhibit the following characteristics: One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP; The misacylated Cys-tRNA(Pro) is not edited by ProRS.; As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine; Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). The designed protein sequence is ",
    "response#1": "RFEDRGNRNFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFIMKDAYSFHTTQESLQATYDVMYQVYSNIFNRLGLDFRAVQADTGSIGGSASHEFQVLASSGEDDVVFSTESDFAANIELAEAIAIGERQAPTAEMCLVDTPNAKTIAELVEQFNLPIEKTVKTLIVKGADENQPLVALIIRGDHELNEIKAQKHPLVADPLEFADETEIKAKIGSGVGSLGAVNLNIPAIIDRTVALMSDFSCGANIDGKHYFNVNWERDVAIPKVFDLRNVVEGDPSPDGKGTLQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVVASAIEQFTWAPSVDDAKTIRAVAERLSVQEEKIIKSLLVSIDDEWALILLRGDHELNDIKLKHALGASDIRLATEEEVLEVIGTEVGTIGPVDVKGVKVIADHGIKSIVNGVCGANESNKHFIHVNETPNVKTVDEVAEFLKVEKKQILKSVLFKADDGYVLAVVRGDREVNEVKVKNQVDCLVLELASPQEVEEVLGAEYGSIGPVGVNIPVIADLEVKHVLNAVTGANETGYHLTGVNTPNVKTVDEVAEFLKVEKKQILKSVLFKADDGYVLAVVRGDREVNEVKVKNQVDCLVLELASPQEVEEVLGAEYGSIGPVGVNIPVIADLEVKHVLNAVTGANETGYHLTGVNDAKTIRAVAERLSVQEEKIIKSLLVSIDDEWALILLRGDHELNDIKLKHALGASDIRLATEEEVLEVIGTEVGTIGPVDVKGVKVIADHGIKSIVNGVCGANESNKHFIHVNE",
    "response#2": "FVDRGERPFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAVEGSSFPQVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAGPGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVAGDPSPDGQGRLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTRVVAAAIEQWQAFQDAEVVSQKLMLRAGFIRKVAAGIYSYLPIGLRVIRKVEDIVRDEMNRAGALELTMPLVQPAELWDETGRWEQMGAEMLRFKDRHQRDFALQPTSEEVVTDIARQELKSYRQLPKNFYQIQTKFRDERRPRFGVMRGREFTMKDAYSFDRDVAAAGRSYDAMYAAYCRIFDRLGLTYRAVAADTGAIGGDRSHEFQVIADTGEDAIVYCPGSDYAANIELAEALPLLATRAAPAQALEKTPTPGRTTCEEVAKLLGVPLETTVKSLVLATDDVDDTNKPAGVTVWLLLVRGDHELNEVKAGKIPGLKAGFRFATETEIAERFGCQPGYLGPVGVNKAVKIVADRTVANMADFICGANEADFHLTGTNWGRDLPEPDLVADLRNIIEGDPSPDGRGRLAIQRGIEVGHVFYLGTKYSQSMNATFLDENGKPKHFEMGCYGIGVTRILGAAIE",
    "response#3": "RFEDRGNRNFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFIMKDAYSFHTTQESLQATYDVMYQVYSNIFNRLGLDFRAVQADTGSIGGSASHEFQVLASSGEDDVVFSTESDFAANIELAEAIAIGERQAPTAEMCLVDTPNAKTIAELVEQFNLPIEKTVKTLIVKGADENQPLVALIIRGDHELNEIKAQKHPLVADPLEFADETEIKAKIGSGVGSLGAVNLNIPAIIDRTVALMSDFSCGANIDGKHYFNVNWERDVAIPKVFDLRNVVEGDPSPDGKGTLQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVVASAIEQFAATNRFHDAKIISHQLMIRSGMIRKTSSGLYVWLPTGMRVLKKIKNIITTEMEKINALEILMPIIQPEYLWKESGRLNLYGEELLRFLDRRKNQFILGPTNEEVVTNFIGSEIHSYKQLPLTVYQIQTKFRDEIRPRFGIIRTREFTMKDAYSFHINQSCLENTYNKFYDSYINIFKKMNLNFCAVKADSGSMGGNISHEFQAFSQNGEDEIVFSNDKLYSSNMNMAESIETIDFFKKKYSSCLIKNKTNTKKSIIMSEKLNTPLINQIQTFLIQTKINDITSIAALLIRGDHELNFFKVEKIDIINKPLVFLNEKEVISLIGVKKEFLGPLGLKVPIIADISTFNMKNFTIGSNINKHFFINVNWNIDLPMPIFKDIRKVTKNDLSPNGSGYLNIKQSIEIGHIFQLGQKYSRKIQQSVKIKNGNLKNLYMGCYGIGITRIAAAVIE"
  },
  {
    "reference": "MARYRCCRSQSRSRCYRQRRSRRRKRQSCQTQRRAMRCCRRRSRLRRRRH",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be able to regulate sperm DNA condensation.2. The protein must exhibit the following characteristics: Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. The designed protein sequence is ",
    "response#1": "MMGMM",
    "response#2": "MIGRFYIQMCT",
    "response#3": "MIP"
  },
  {
    "reference": "MVEIDDRPTTRAPDTPGTPDWVRCEGCAALVYGKRFTRLLRVCPDCGRHDRLTAHQRLEHLLDPGSAVPLEEVEPLDDPLAFTDTRPYPERLEQARAATGLREAVVCVRGSIEGKPLVVAAMDFRFLGGSLGCGVGALIAGAARTSLRERVPLLIVTASGGARMQEGVLSLMQMAKTAQALAELDEAGILTVSLITDPTYGGVAASFATLTDVILAEPGARLGFAGPRVIEQTIGEKLPEGFQTAEFLLAHGMVDDVVARPALRGVLARLLSLRPAPGPDADGRAAADAQAAADAQAAADAQAAADAQAAADGRAPAGPRPGSLLRRAEDLPDRDSWEAVRLARHADRPTSLDYIGHLVDDFHELHGDRLHEDCPAVVGGPGRLGGRPVMLIGHHKGGADLSERRRRLFGMPTPGGYRKAARLMRLAAKLGMPVVTLVDTPGANPGPDAERGGQALAIAENLRLMSQLPVPIVTVVVGEGGSGGALALAVADRVLVSANGVYSVISPEGCAAILWKDPKAVPAAASALRMHAGELLRLGIVDGVVPEPPGGAHSDHAEAALLLGDALSEALDELVPWEPGRLLRERRERFHRFGVETPDLPEETPEEVHR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have a high affinity for Zn(2+).2. The designed protein must have at least one CoA carboxyltransferase C-terminal domain. The designed protein sequence is ",
    "response#1": "QPQLKVAPAAPAAGGAVAGPPWETVQLARQAGRPTIRDTIANCCDDFVELHGDRLFGDDHGLIGGFATIGGLRVMLIGHQKGKTVEENIAVNFGMANPEGYRKALRLMRLAERYGLPVVSLVDTPGAYPGLDAEARGQAEAIARNLTVMATLRTPFVVIVTGEGGSGGALGIAVGDRVLMLQNAVYSVISPEGCASILWRDGKKAPEAAAALKITAGELLKLGIIDGIVPEPAGGAHLDPKAATAAIKLAILGALTR",
    "response#2": "PLTEPPPAVRGTRDTVALARNIGRPTTEEYIGHVFDDFVSLHGDRVFGDDQALTGGIARLGGRNVVVLGHQKGHDTQALVRSNFGMPNPEGYRKALRLMRLADRFGLPVVAFIDTPGAFPGIGAEERGQSLAIAECILELSRLRVPVVSVVTGEGGSGGALALGVANSVLMLENAYYSVISPEGCSTILFGTADRAGQAADALRLTAGDLLRLGVVDAIVPEPPDGAHTAPYPTALNVKTALVRTLGE",
    "response#3": "IMTKCPSCRTIMYTKDLKKNLSVCRTCGHHHRMSARERIDSIIDDGTFKEAFASVKGGNPLQFPGYEEKLESDRKKTGLNEAVVTGEGKINGYPVVIAVMDPNFRMASMGSAVGEKLTRAIELATETSVPLLIFAASGGARMQEGMLSLMQMAKTSAALERLNRAGGLYISVMTNPTTGGVSASFASLGDYNFAEPKALIGFAGRRIIEQTIRQELPEDFQTAEFLLEHGQLDRVIPRLEMKETLTKLLAMHS"
  },
  {
    "reference": "MKQRPTGPDTTPRNRRGFGRDTAVASVCGLVVALMVGASYAAVPFYNWFCRVTGFNGTTQVAAAAPQGGALKRTIAVRFDSNTNGLPWSFAPETTEIEIPIGQVTTVYYKVTNRAAHETTGQAAYNVAPLTVGAYFQKINCFCFTDQTLGPHETRELPVVFYVDPALATDSDNDTLNSITLSYTFYPLRSAAPKPLAASEAGPRQGAL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should contain Periplasmic topological domains.2. The protein should have a Helical; Signal-anchor for type II membrane protein domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "WRIDYNAAVAYDFLAAEDVNKDKIQDILFLYKNTNSSRGNSSFSCADEGFSCPCTFVAAVSGASGSVLWERPVAQDRAFVECGILQPRGSAAPSACVVLGRPGSLVAVDTLTGKTLWSQPSSFGGNASVLSPLLRVPDLDADGAPDLLVLIQEENQVNGSIYSGGTGQQVSPPDSLGVDGTSGSILHVTRAGAHYVLIPCGTALCSRSVKGLYEKVSRRDSPLKSDPLWEDMLSAASHRLVVHSSGAIRYLMNVPGKAGDDLLLVSTEAYMLLDGQDLTPRWTFGTTQVLRKPVLGYYKPDTPAVLVENGTGPDRQVLLLDLGSGAVLWSQALPGLPGDPPSASLPTADHRSAFFFWGIHEPTDSNQTEPGAAGRRLYMLHPTLPGVLLELDNVSVPIVAFQVVLLEPGRHAACILLTGPASPSPPGLVSVTKHKVQDLVLAGRVVHLAEGGAESDQAVRDRLSRLRYR",
    "response#2": "WRIDYNAAVAYDFLAAEDVNKDKIQDILFLYKNTNSSRGNSSFSCADEGFSCPCTFVAAVSGASGSVLWERPVAQDRAFVECGILQPRGSAAPSACVVLGRPGSLVAVDTLTGKTLWSQPSSFGGNASVLSPLLRVPDLDADGAPDLLVLIQEENQVNGSIYSGGTGQQVSPPDSLGVDGTSGSILHVTRAGAHYVLIPCGTALCSRSVKGLYEKVSRRDSPLKSDPLWEDMLSAASHRLVVHSSGAIRYLMNVPGKAGDDLLLVSTEAYMLLDGQDLTPRWTFGTTQVLRKPVLGYYKPDTPAVLVENGTGPDRQVLLLDLGSGAVLWSQALPGLPGDPPSASLPTADHRSAFFFWGIHEPTDSNQTEPGAAGRRLYMLHPTLPGVLLELDNVSVPIVAFQVVLLEPGRHAACILLTGPASPSPPGLVSVTKHKVQDLVLAGRVVHLAEGGAESDQAVRDRLSRLRYR",
    "response#3": "WRIDYNAAVAYDFLAAEDVNKDKIQDILFLYKNTNSSRGNSSFSCADEGFSCPCTFVAAVSGASGSVLWERPVAQDRAFVECGILQPRGSAAPSACVVLGRPGSLVAVDTLTGKTLWSQPSSFGGNASVLSPLLRVPDLDADGAPDLLVLIQEENQVNGSIYSGGTGQQVSPPDSLGVDGTSGSILHVTRAGAHYVLIPCGTALCSRSVKGLYEKVSRRDSPLKSDPLWEDMLSAASHRLVVHSSGAIRYLMNVPGKAGDDLLLVSTEAYMLLDGQDLTPRWTFGTTQVLRKPVLGYYKPDTPAVLVENGTGPDRQVLLLDLGSGAVLWSQALPGLPGDPPSASLPTADHRSAFFFWGIHEPTDSNQTEPGAAGRRLYMLHPTLPGVLLELDNVSVPIVAFQVVLLEPGRHAACILLTGPASPSPPGLVSVTKHKVQDLVLAGRVVHLAEGGAESDQAVRDRLSRLRYS"
  },
  {
    "reference": "MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGESDTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQQPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIADEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCWPWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPLLSGNYEITELDPANDQRAS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that The combined action of Cho and UvrC broadens the substrate range of nucleotide excision repair. The designed protein sequence is ",
    "response#1": "APFRPTGDQPTAIASLIESLQGEHKFQTLLGATGTGKTFTMASVIAELGRPTLVLAHNKTLAAQLCNELRQFFPENAVEYFISYYDYYQPEAYIPVTDTYIEKTASINDEIDMLRHSATRSLFERRDVIVVASISCIYGLGIPSQYLKAAVPLQVGAEYDPRLVIRDLVNVQYSRNDVELQRGRFRLKGDVLEIVPAYEDRVIRIEFFGDEVEALRLIDPVSGEILQSLDRISIYPARHFVTPEETLEKACQQIQTEMEQQVAFLEKNNLLVEAQRLSQRTRYDLEMLREVGYCNGVENYSRYLADRQAGEPPECLVDYFPEDWLLVIDESHVTIPQLRGMYNGDQARKKVLIDHGFRLPSAADNRPLKAEEFWQKVKQCVFVSATPGVWEIEQSEARVIEQVIRPTGVLDPEIFVPLQVGAEYDPRLVIRDLVNVQYSRNDVELQRGRFRLKGDVLEIVPAYEDRVIRIEFFGDEVEALRLIDPVSGEILQSLDRISIYPARHR",
    "response#2": "ESLQGEHKFQTLLGATGTGKTFTMASVIAELGRPTLVLAHNKTLAAQLCNELRQFFPENAVEYFISYYDYYQPEAYIPVTDTYIEKTASINDEIDMLRHSATRSLFERRDVIVVASISCIYGLGIPSQYLKAAVPLQVGAEYDPRLVIRDLI",
    "response#3": "VFAIAQKEGISCIQVFFFRMGQNWGNRAYFPKADPSFSSSEILASFLSQFYDDKPLPKLILLSESIEDKTLLAEAFSLKANRKISLSLPKQGERKTLVNYAYINAHETLGHKLAETATHTKLFQGIAEIFQLPHTPCRIEIYDNSHIMGTNAVGAMIVAGKNGFIKNQYRKFNIRSTDITPGDDFGMMKEVIKRRFSRLIKEHGLPHESKNIQSKDDASFPIWPDLILIDGGKGQINSVHTIL"
  },
  {
    "reference": "MNRIFRNTIFYLLIFLVIIGVVSFFNGSNQPTEHIKYDEFISELEQGNVNNFTLQPERGVFEIRGQLEGYEEGKYFLTYVWNSEAQLAELRDAAAQADIEVLPAKETSGWVTFFTSIIPFIIIFILFFFLLNQAQGGGSRVMNFGKSKAKLYNDEKKKVRFKDVAGADEEKQELVEVVEFLKDPRKFAELGARIPKGILLVGPPGTGKTLLARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFENAKKNAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFGANEGIIIVAATNRPDILDPALLRPGRFDRQITVDRPDVNGREAVLKVHARNKPLSESVNMRAIAQRTPGFSGADLENLLNEAALVAARQNKKKIEMEDIDEATDRVIAGPAKKTRVISKKERNIVAFHEGGHTVIGLVLDEADIVHKVTIVPRGQAGGYAVMLPKEDRYFMTKPELLDKITGLLGGRVAEEIVFGEVSTGAHNDFQRATGIARKMVTEYGMSDKLGPLQFGQAQGGQVFLGRDLHNEQNYSDAIAYEIDLEIQRIIKDCYARAKQILTEHRDKLDLIANTLLEVETLDEEQIRHLADHGKLPDRSGPVTVDAGDDVKVTINSKKDETALKNAENSGEASENTPNEDKPKE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.2. The protein design should emphasize Basic and acidic residues compositional bias.3. A protein with metalloendopeptidase activity, ATP-dependent peptidase activity, zinc ion binding is needed to enhance protein catabolic process, proteolysis in a specific context.4. For general function, the protein need meet that Plays a role in the quality control of integral membrane proteins. The designed protein sequence is ",
    "response#1": "VLLVGPPGTGKTLLAKAVAGEASVPFFSISGSDFVEMFVGVGASRVRDLFKQAKESAPSIVFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFDDNSAVILIAATNRPDILDPALLRPGRFDRTVTVHFDADLDGLAKRTVGMSGADLANVINEAALLTARQNGTMINGAALESADEIIDRVVERAIFK",
    "response#2": "VLLVGPPGTGKTLLAKAVAGEASVPFFSISGSDFVEMFVGVGASRVRDLFKQAKESAPSIVFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFDDNSAVILIAATNRPDILDPALLRPGRFDRTVTVHFDVDLAQLASTTPGMVGADLRNLVNEGALLAARRDHKAVTTGDVKLAKARGEIVEDVDLHSVATQTPGYVGADLENVLNEAALVAARQNKAEINAADIDR",
    "response#3": "VLLVGPPGTGKTMLAKAVAGEAGVPFFGLSGSDFVEMYVGVGAARVRDMFQQALQRSPAIIFIDELDALGKVRGSGAPGGHDEREQTLNALLVEMDGFGTDHSVIVMGATNRPETLDPALMRPGRFDRHVLVA"
  },
  {
    "reference": "MPARKRFGQHWLRSEAILDRIVAAAELRPSDRVLEIGPGRGALTQRLLAAVDGLVAVELDRDLIGQLQQRFGQAENFCLLEGDILQLDWTAAIADRPRFANPSKVVANIPYNITGPILQSLLGTIAQPRRPAFERLVLLVQQEVADRLCATPGQRAYGALSVRVQYLASCERVCAVPPKSFSPPPKVQSTVICLKPRPWPQVCNNPGRLEKLLNQGFSAKRKMLRNNLKSLYSSEQIEAAFAAHQIAPEARAETLSIDQWIGLCTDLGDPTDSAINPTA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. The designed protein sequence is ",
    "response#1": "AVGEFLKPLEKAAAEGIREALEIGPGAGSITLPAAEVLDRIVAVELDNRLASALSRLAPARVAVITGDGVSHAAASQAPLVFSNTPFNLSPAIVEALAVNNRVAAAVLGVQYEVARRMTARPGSRDYSRLSVLVSLVFHAELAGVVRPQAYYPRPQVLTAVVTLRRRR",
    "response#2": "AVGEFLKPLEKAAAEGIREALEIGPGAGSITLPAAEVLDRIVAVELDNRLASALSRLAPARVAVITGDGVSHAAASQAPLVFSNTPFNLSPAIVEALAVNNRVAAAVLGVQYEVARRMTARPGSRDYSRLSVLVSLVFHAELAGVVRPQAYYPRPQVLTAVVTLRRRR",
    "response#3": "AVGEFLKPLEKAAAEGIREALEIGPGAGSITLPAAEVLDRIVAVELDNRLASALSRLAPARVAVITGDGVSHAAASQAPLVFSNTPFNLSPAIVEALAVNNRVAAAVLGVQYEVARRMTARPGSRDYSRLSVLVSLVFHAELAGVVRPQAYYPRPQVLTAVVTLRRRR"
  },
  {
    "reference": "MEPSIKYPIYEWELLQDTYYQKSAIGKAEWEYIDPVDFMFAVAPCGGAIAITRSESNLQSNYKYDQVPMYSICVFCLSGQLLQTLTWDKTSLVGMGWNENEELIVVSKQGQVRVYNLLGEFHQFSLGKGVENIGIRECQFSEGGVFALLQNDTFISITGFEEPWRKTYASIPFNTLEYYNIDSWALIPNPFSPDLGMDIVVTVGPHILQIDEQDSQLHSISSLQHVSHISISPNARYLALYESVGKVRVISSDFSKELLDLRLPETVAEASLKQMAWCGNDAVVLVHENLLTLVGPFGGSVPYLYNHTPIVSTEVDGVRILTKDSSEFLRKVPAPLENIFHIGSKTPGAKLVEAFQKMKLKSVFAEKMLLELKDELHDAVDTCVQASLNEFSIEWQKVLLEAASLGKNSLRMYNHQEYVDVCRELRVLNAARDPNVGIYITHEEYLHLGLERLIQRFSCRQLYGLAVQASMWMQIPCDWVYIQWAQTYIKQSSEPEEVVLDNIVKRLSSRKYISYEKIARTAYQEGRLILSTKLLDFEPLAKHQVMLLLNMEAYEQALKKVIETMDNNLIIYVVLQIKQQMAIASFFQILNEYPDAVKVYVEFAKKNDRKTLHDFFYQDDNKQGIAVLAVEDTLKTATVNQRITSLKSAAKVCSESKELSLEEKCLGDEIKLLQLQQTYEDQFTGNFVGLTVNEVVVKLIQINQTQRANKLRSDFQIPEKRFAWLKLRALVAIRDWVQIEQWAGSMRRSPIGFEPFVTEILSAGNKKE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must possess actin binding to support vesicle docking, Golgi to endosome transport, vacuole fusion, non-autophagic, endosomal transport, late endosome to vacuole transport under certain conditions.2. The protein must exhibit the following characteristics: Required for vacuole biogenesis, stability and to maintain vacuole morphology .. The designed protein sequence is ",
    "response#1": "LYTLVTSLDMRLISILRPSNHTLHTMDLQILFEVVRAQLTDLLLDKVLWFPICFQKFNDNGFLYALIKVLPNNTIMMVISSQKNAFDILNEFVRC",
    "response#2": "PLLYGLVVAGGRLVSVIRPKKHSLHPGDLQLIFNMIFEADGVKAGGGESWIPICLPGFNSRGYLYMYVSFLDLHDHLPEDDKDINKDDAVAIILISADKESFFVLREMRDSVVV",
    "response#3": "PLLYGLVVAGGRLVSVIRPKKHSLHPGDLQLIFNMIFEADGVKAGGGESWIPICLPGFNSRGYLYMYVSFLDLHDHLPEDDKDINKDDAVAIILISADKESFFVLREMRDSVVQ"
  },
  {
    "reference": "MARLAGVAALSLVLVLLGAGVPRPAAAAAAKTQVFLSKLPKALVVGVSPKHGEVVHAGENTVTVTWSLNTSEPAGADAAFKSVKVKLCYAPASRTDRGWRKASDDLHKDKACQFKVTVQPYAAGAGRFDYVVARDIPTASYFVRAYAVDASGTEVAYGQSSPDAAFDVAGITGIHASLKVAAGVFSTFSIAALAFFFVVEKRKKDK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Imports nitrate with high affinity when expressed with NTR2.1, NTR2.2 or NTR2.3 in a heterologous system (Xenopus oocytes).; Required for high-affinity nitrate transport; Acts as a dual component transporter with NTR2.1, NRT2.2 and NRT2.3; Involved in the regulation of NRT2.1, NRT2.2 and NRT2.3 expression, and in both, HATS (high-affinity transport system) and LATS (low-affinity transport system) activities in plant roots2. The ideal protein would be one that plays a critical role in response to nitrate, nitrate transmembrane transport through nitrate transmembrane transporter activity within plasma membrane.3. Design the protein with a signal peptide incorporated.4. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "TAKLGFIALTDAAPLIIAKEKGFYAKYGMPDVEVLKQASWGTTRDNLVLGSASGGIDGAHILTPMPYLITMGTVTDGKPTPMYILARLNVNGQGIQLGNNYKDLKVGTDAAPLKEAFAKVTDPKVAMTFPGGTHDMWIRYWLAAGGMEPGKDFSTIVVPPAQMVANVKVNAMESFCVGEPWPLQTVNQGVGYQALTTGQLWKDHPEKAFGMRADWVDQNPKAAKALLMAVMEP",
    "response#2": "TFHLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGAVCDLLGPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFNGQIIGLVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGILVLTLGQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDNVIAEYFFDRFHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQTLGGFFCLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGNFGSGLTQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTILISLQRVAIGYLLAACTGILVGGVLGMSKFLGKGLDPVIQVLRTVPPLAWFPISLMVFQDANTSAIFVIFITAIWPIIINTAVGINQIPDDYNNVARVLKLSKKDYILNILIPSTVPYVFAGLRIAVGLAWLAIVAAEMLKADGGIGYFIWDAYNAGGDGSSSQIILAIFYVGLVGLSLDRLLFQ",
    "response#3": "TAKLGFIALTDAAPLIIAKEKGFYAKYGMPDVEVLKQASWGTTRDNLVLGSASGGIDGAHILTPMPYLITMGTVTDGKPTPMYILARLNVNGQGIQLGNNYKDLKVGTDAAPLKEAFAKVTDPKVAMTFPGGTHDMWIRYWLAAGGMEPGKDFSTIVVPPAQMVANVKVNAMESFCVGEPWPLQTVNQGVGYQALTTGQLWKDHPEKAFGMRADWVDQNPKAAKALLMAVMEGS"
  },
  {
    "reference": "MQVNLEDVKIEPSWKEALKGEFLSLYFGDIKEKLICAKRAGIVYPPSNLIFNAFNLTPFDTVKVVILGQDPYHGPNQAMGLSFSVPNGVRIPPSLVNIYKEIYDDLGIREPNSGDLTYWAKQGVLLLNASLTVGANMANSHSGFGWQIFTDAVIKILSRERENIVFLLWGNPAKAKIPLIDTNKHLVLTAAHPSPLARGAFFGCRHFSKTNFYLTQHAKTPIDWDLNNFQI",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Proton acceptor of the protein must be engineered to enable effective catalysis of Hydrolyzes single-stranded DNA or mismatched double-stranded   DNA and polynucleotides, releasing free uracil..2. The designed protein should have uracil DNA N-glycosylase activity within cytoplasm to regulate base-excision repair. The designed protein sequence is ",
    "response#1": "PLENVKVVIIGQDPYHGPGQAHGLCFSVQPGVPTPPSLQNIYKELNRDLNIPIPNNGYLQRWAEQGVLLLNTSLTVEQAKAGSHANAGWQPFTDRVIEVVNERCERLVFLLWGSHAQSKQKLIDPQRHLILKSAHPSPLSAYRGFLGNGHFSRTNKFL",
    "response#2": "FGDVKVVILGQDPYHGPNQAHGLCFSVLPGVKTPPSLVNIYKELAQDIPGFQIPPHGYLQSWAQQGVLLLNTVLTVEQGMAHSHANTGWETFTDRVIDALNQHRNGLIFLLWGSHAQKKGQMIDRQRHHVLMAPHPSPLSAHRGFLGCRHFSKTNQLLR",
    "response#3": "NLTPFHDVKVVILGQDPYHGANQAMGLSFSVPSGVRVPPSLVNIYKEIYADLGIKEPNSGDLTKWAKQGVLLLNSTLSVSAGAANSHASFGWQGFTDAVIRKISENLQNVVFMLWGNPAKAKAPLIDASKHLILEAAHPSPLARGAFFGCRHFSKANIYA"
  },
  {
    "reference": "MPNGKYAAHRLQQVRKDARWKDTGYSRRTLGLDIKSDPLSGAPQGRGIVLEKVGVEAKQPNSAIRKCVRIQLIKNGRQATAFCPGDGAINFIDEHDEVTVERIGGRMGGAMGDIPGVRFKVIAVNNVSLREMVIGRKEKPRR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein with structural constituent of ribosome, rRNA binding is needed to enhance translation in a specific context. The designed protein sequence is ",
    "response#1": "MPSINGEITAKEVRLISESGEQLGVVSVREALAMAEGQDVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEV",
    "response#2": "MI",
    "response#3": "LSRRHAAEKREVLPDAKYGDLVLTKFMNNLMIDGKKSVAETIVYNALTRVETKIKRAPIEVFHEALDNIQPSVEVRSRRVGGATYQVPVEVRPERRQALAIRWLIKAARSRNENTMEERLAGELLDAVQSRGTAVKKREDTHKMADANR"
  },
  {
    "reference": "MKICLASSNAHKIAEFRDMARDAGLDCEIFGAGGLPGFEPPEEDGATFAENAMIKARAARAIAPRDCHIMADDSGLCVDALGGAPGIMSARYAGEKGAGADEANNRKLLKELENVPDAARSARFVCSIALICPDGREEIFGGEIEGVINRGARGKRGFGYDPLFFLPERGMTTAELAPAEKNSISHRGKAFSKAAEFIKSQQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The Proton acceptor of the protein must be engineered to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+).2. The designed protein must possess ITP diphosphatase activity, metal ion binding, dITP diphosphatase activity, ribonucleoside triphosphate phosphatase activity to support nucleotide metabolic process, purine nucleoside triphosphate catabolic process under certain conditions.3. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+), substrate binding.4. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.5. The protein requires Mg(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "MAVLGIDPGSRITGYGVIRQVGRQLVYVGSGCIRTSADDLPSRLKLIYAGVTEIITQFNVDTFAIEQVFMAKNADSALKLGQARGAAIVAAVNRDLPVFEYAARQIKQTVVDYGLVKNEQVQTMVSMLLRLTESLQLDNEADALAVAICHYHTHRAAASLNAGGKRYTNNNRYKK",
    "response#2": "MHLDTLVIATNNAHKVNEFAQIVSDLPYNVVSLKDIGCDVDIPETADTIEGNAIQKAHAAYTLLGYPVLADDSGLEVDALGGAPGVHSARYAGEHGNNEANNRKLLHELAMVPMEKRTARFRCVIAVCFPGRADPANVKIVTHGICDGLIAESARGSNGFGYDPIFYYPDLNKTFAELTKEEKHQISHRGKAVRNMLTELKTHFS",
    "response#3": "MILIFATHNLHKVEEIKALLPPHIQLLTLDDIGCHEELPETADTLEGNAALKASYVFEHYGLACFADDTGLEVEALDGAPGVYSARYAGEGRNDEANMQKLLADLQGQTDRRARFKTVIALNLNGKQHLFEGICRGSILTEPRGERGFGYDPVFVPDGYDRTFAEMTLQEKSRISHRGLATQALLTFLKTYHP"
  },
  {
    "reference": "MDVLVPFAAREPKTRLAGTLDADERAAFARAMLLDVCDAVEAAGGEPTVLATEPLAIEQPVVVDKRPLTPAIDAHLSPPAAVVMADVALVTPAALRRLFATTGDVVIAPGLGGGTNALVVRADGFTVDYHGTSVRDHRRAAEGAGIDARSVDSFRLAVDIDEPTDLAEVLLHGDGRSADWLKNAGFRLAETDGRTTVERRG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein have enhanced (2S)-2-phospholactate + GTP + H(+) = (2S)-lactyl-2-diphospho-   5'-guanosine + diphosphate.2. A protein with phospholactate guanylyltransferase activity, GTP binding is needed to enhance F420-0 metabolic process in a specific context.3. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis, coenzyme F420 biosynthesis pathway to avoid interference with native metabolic processes.4. The protein must exhibit the following characteristics: Guanylyltransferase that catalyzes the activation of (2S)-2- phospholactate (2-PL) as (2S)-lactyl-2-diphospho-5'-guanosine, via the condensation of 2-PL with GTP; It is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor.. The designed protein sequence is ",
    "response#1": "MAXGFAXGXIXGXKXDIXSXIGXAXGXDXFXDXEXALXDXAXSPXPXXGEXESXSXFXXXDXYKLPXLXXAAXAXPXPXXLLVAXDPDREGLXAXAIXDXIXAFXXXLXRXDXEXGXGXGXAXDPXPEXAXVDRDGNPXXXXXXXXXXXXXRXRMXXXMXRXXXXXAGRSNAGKSSXXXMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRXEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX",
    "response#2": "MXLVXGLXXLKXPXLXGVXEXKXPXXXSXGXXXPXPAAXXC",
    "response#3": "MSTAAPLPDGAVSAADVLARDRVHDALRRRYAPRFEPVAGGTRWLYFTGNSLGLQPRAAKRAVVDELDDWARRAVEGHFRHPRPWWDYHERLTDPLAGLVGAAPDEVTVMNTLTVNLHLLLVSFYRPTATRHAILVEAGAFPSDRYAVASQIAFHGFDPATALVELAPRPGEHTLRTEDIEAVLARHGDDTALVLFGGVNYYTGQLFDLRAITAAAHAHGAMVGFDLAHAVGNLPLRLHDWDVDFAVWCTYKYLNSGPGAVAGAFVHERHAHGTDLPRFAGWWGHEPGTRFRMGPEFDPAAGADGWQLSNLPVFPMAPHRAALELFDEAGMAALRAKGRALTGFLAGLLADRLPDRVEIVTPADPARRGCQLSIRVTGRAAALHDRLTEAGVACDWREPDVIRVAPAPLYNTFEDVWRFVELLG"
  },
  {
    "reference": "MSNKFKKILVTCALPYANGPIHIGHMLEHIQADIWVRYQRMRNNEVWFISSDDAHGTAIMLKSEKLGITPIKLIKKIKEEHIIDFSNFNISHDNYHSTHCVENLFLLRKIFLSLSEQKLINEKKIFQFYDNTKKMFLPDRFIKGTCPFCSSKNQNGDNCEICGSIYEPTDLVNPISVISNSVPILKNTTHLYFNLPLFTNMLNKWIHSGILEKSVAKKTEEWLKSGLKEWGISRDAPYFGFKIPKFDKKYFYVWLDAPVGYISAFKNFCTKNKKVDFNEFWKKESKCELYHFIGKDIIYFHTLFWPAILEASSYRKPNGIFVHGHLTINGLKLSKSRGFLIKASDWIKCFDSDSLRYYYASKLSNNINDIEINLEDFIQKINSDIVNKLVNLASRNASFIHKYFDGYLADSLGDCNLYQDFIDISKKIANFFENRQFSSVIRECMKLLDLANQYINQREPWKIKQDNFNKLHTICTVGINLFRLVVIFLKPIIPDLAKKTEYFLISDLTWKGIDKPLLSHKIKKFKKLYNRINHDKILQLSLLCK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein with metal ion binding, ATP binding, methionine-tRNA ligase activity functions is required to enable methionyl-tRNA aminoacylation.2. The protein should have a high affinity for ATP.3. Incorporating a 'KMSKS' region motif is critical for the successful design of the protein.4. Zn(2+) is required for the proper folding and stability of the protein.5. We need a protein that can perform ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-   methionyl-tRNA(Met) with high efficiency. The designed protein sequence is ",
    "response#1": "IPETGTSGDAEAWMANELAERLPRLIEFYEAMEFRKAAAETRAIWAAGNEYLTKAEPWVKYKNDVDGAAVGVRAGINLAAIFGIIAQPIIPDAAKKILDALGIPEENRKMPAGSKPEDFAALLDAIPRGHAISPPDVLFQKIEDSQVEEWTAQFGGGR",
    "response#2": "LDDFVARVNSDLVGKYVNIASRSAGFITKRFNGQLAPATAELPAIKGIQEAASRIAELYEAREFGKAMREIMALTDAANQYVDSVKPWELAKQEGKEVELHAACTNALNLFRLLTVLLKPILPIVAEKVEKFV",
    "response#3": "LDDFVARVNSDLVGKYVNIASRSAGFITKRFNGQLAPATAELPAIKGIQEAASRIAELYEAREFGKAMREIMALTDAANQYVDSVKPWELAKQEGKEVELHAACTNALNLFRLLTVLLKPILPIVAEKVEKFV"
  },
  {
    "reference": "EFHFNRYLTRRRRIEIAHTLCLSERQVKIWFQNRRMKWKKDHKLPNTRCDLPTLPRPLPARLGKHKL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for stability and solubility when expressed in nucleus where it will be involved in regulation of transcription by RNA polymerase II, embryonic skeletal system morphogenesis. The designed protein sequence is ",
    "response#1": "MLPLVCKGEGSLLFEATSLLLWGHTGLSLELRARTVVEMLLHRHYYLQGMIDSKVMLQAVRYSLCSEESPEMTNLSFATLEAIFDADVKATCFPTSFSNVWHLYALASILECNIYSIYPMRNIKIRPYFNRVIMPRCSTHVTSMLHIMWAGQPLTSHLFRHQYFAPVR",
    "response#2": "AHYWKDFHPRWPFLHRGTLKVDEAPVELLLAMITMGMHFVGDAFAYSIAVSIHSTLRFSIYTHPDFKPPASLWVYQALLIAEIFEKMTSTVEQHNLSQIFHGVTIESMQNGLSSKDTVTEKIPKNMTGTNIAQHKWHQWVDREASKRIAFFSFVLDSQHVILFGYRPLIDITSVGLPLLCDEALWNADSYEAWTSLLNENDPPHFFPVLKMFLTNEHLPPKLSPWNMMIVLHGLTTIGWILSRENLGIVENIMQNNGTNLKNWRILLKASYKFN",
    "response#3": "QPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVV"
  },
  {
    "reference": "MDKYAVWGNPIAQSKSPQIHQIFANQTQQQMEYVAMLGDEQDFEQQLRVFFEKGAKGCNITAPFKERAFQLADLHSERCLTAEACNTLKKLDDGRLYGDNTDGAGLVSDLQRLGWLKPEQTILILGAGGATKGVLLPLLQAKQHIVLANRTLSKAEDLAQKFAQYGQIQAVELDNIPVQSFDLIINATSSGLHGQTVQMNPEILQNATALYDMQYAKQADTPFVALCKQLGKQNVSDGFGMLVAQAAHAFHLWRGVMPEFEALLEQEWL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).2. We are looking for a protein with broad substrate specificity for NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH.3. The designed protein should be optimized for shikimate 3-dehydrogenase (NADP+) activity, NADP binding activity in order to improve chorismate biosynthetic process, aromatic amino acid family biosynthetic process. The designed protein sequence is ",
    "response#1": "QGQHILIIGAGGAARGVLFPLLEFGCKITITNRTFSRAIQVANNFSAIGSIRPAEMKVLNSPEFDLIINATAM",
    "response#2": "LTGKKVLLLGAGGVARAIGVGAKDRGAELLVTSRTYERAVELAAKLEGEALAWEDRHSANADVLVNCTPVGMHPKMNETPFEEAV",
    "response#3": "LTGKKVLLLGAGGVARAIGVGAKDRGAELLVTSRTYERAVELAAKLEGEALAWEDRHSANADVLVNCTPVGMHPKMNETPFEEAK"
  },
  {
    "reference": "MKLNSLIKDIVQDKNDLSDININKICTXSKXAKXGSLFVAINGTXNXGHDFINEAIENGASAVISNGKNVGNSSVPNIKVSNPRLAASRVSAEFYXYPSKELKIIGITGTNGKTXTASLVYSILKSANXKVAQIGTXGLIADGFIKNKTLTTPDPITLHKILRDLSDNNFSHVVMEVSSHALDQSRVADIDFDISVFTNLSXEHLDYHNDMETYFRAKSKLFRMMPITSTSIINVDDEYGVRIXNESSAPVISISSKNKSDIFYKKINYSLSGINGLIRGGNEEISFNSRLIGEFNAENXISAASVGIALNIGLKNIENGINSVKVIDGRMEIFKTTDNKNIVVDYAHTPDSYTKVLSTIKKLIGKEKNIYVLFGCGGDRDKSKRNEMGKIAANFAEKLWITPDNPRFENIDNINFEITKELNDDKFEIFNDRGVGLKKAISKMKRDDTLVVLGKGREDYQDVEGKKVEYSDIEIIKKYL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should take into account the physiological conditions of the peptidoglycan biosynthesis, Cell wall biogenesis pathway.2. The protein should have a high affinity for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate.3. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.4. A protein that contributes to cell cycle, cell division, peptidoglycan biosynthetic process, cell wall organization, regulation of cell shape by functioning in cytoplasm with ATP binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, magnesium ion binding.5. The designed protein must have at least one Mur ligase central, Mur ligase C-terminal domain. The designed protein sequence is ",
    "response#1": "MKSIEKLEYDSRKVCKGDVFFALPGVTSDGHDFISVAIKNGASAIVVSESVNLNQEGITVIKVQHTRKAFEVESITSDSRNVDKGSLFVAVEGIFTDGHSYIGKAIEQEASVVVYDKPLIEEYFSRVTYVQVEDSTVALALIASAFYDFPSRKLKVIGITGTNGKTTSAFMIKRVLEEAGYKCGLLGTVSYYYGDREIPGPNTTPDSLELQRLFSEMRDAGVEYVVMEVSAHSLEQRRVAGCQFDAGVFTNLTQDHLDFHKSLENYFASKKKLFEGLDKNAKVAINVDDDWGKRIVALAGVTGTKGKTTTTFMIKEILEKAGKKVGLIGTIATYINGKRIKDSDRTTPESLELQQIFKQMVDAGVETVVMEVSSQSLKLHRVTGCEFDIVLFTNFSEDHISPNEHPNMEDYFNSKLKLFKMCNTGIVNADDLHGAKIPRLFPESNITTYGIDNYANVLAKDITITNSYVDFRVKIKDRNERVKTGIPGRFSVYNSLAAICVASSLGVPPALINKALSSFKGVPGRIEGYRGPAAKPLVVVDYAHTPEALEQILKAVRAHTAGRLICVFGCGGDRDRGKRPLMGAAAVRGADLAIVTDDNPRSESPAAIVADILAGAGAGTRVIHDRAEAIRAAVAEAGADDVVVVAGKGHEDYQILKDRTIHFDDREIARAALEGRKG",
    "response#2": "MKLAELLSAAVPLTLHGDPEITGVTADSRKAAKGFLFAALPGTKVDGRAFAAKAIEAGAAAVIADARDALAVAAANVYGHPSARLTVLGLVNGKPAGLLAGRAAALLGDPTRELAIGITGTNGKTTSAFMIKRVLEEAGITGTNGKTTTSFLVDGGLRAGGWHTGLIGTLGYRIDDRPLDGPRTTVTTPESAELQGLFAALREAGAQAVTMEVSSHALALGRADAVVFDVAVFTNFGRDHLDFHGTLEAYFAAKASLFTAARARRAVINLDDPRGPALVAQARAEGLAVVTTGTADGTAADYRLLELDADGPVTRLTVGTPAGTVRMDLKLPGDHNAHNAVSALAALCREAGVSDAAARAGLAQTSVPGRMQLVAQRENGATVFVDYAHKPGAVIAALGSLRPHVMGRIVCVIGAGGDRDRGKRSLMGQAARDFADVVIVTDDNPRTEDPAAIRAAVMEGAGPEATEIPDRAEAILRGVDALLPGDALLICGKGHESGQEVGGVVRPFDDRAVLRELVEG",
    "response#3": "MKLKDILYKVSLLEIKGNTDLAEITGVTADSRKAAKGFLFAALPGTKVDGRAFAAKAIEAGAAAVIADTRAALSLMAANFYDNPSKKLKLVGITGTSGKTTTSYLVEAGLTAAGRRCGLIGTIETRVAGRHVPSALTTPEAPQLHALFAVMVEDGVDTVVMEVSSHALSLGRVDGTRFAVGAFTNLSQDHLDFHDSLEDYFLAKSRLFAADSAVRADTAVVCVDDAWGVRMADIARAAGSTVVTASTGDDSQWTVSDWAKHEDGTQSFVVADPNGVSLPVSVRLPGRYNVANTVLALAICLELGIEFSEAIQKVGSLTAVPGRCEIMQCTDRLGFHVILDYAHTPDGLEKILKTCKEFTEGRLIGVFGCGGDRDKTKRPIMGKIGTDICDFCVITSDNPRTEEPMEIIRDILKGVERSNYVVEENRREAIKKSIVEAKKGDVIVIAGKGHENYQEVKGVKYPFDDKLVAKEALLNKKR"
  },
  {
    "reference": "MPETGGKIDGKEGARRTQILDVNSRYFGLETSILMENAGRGVADEISRRYGDGKKIGIFCGLGNNGGDGFVVARHLMDGSEVTLYLLGNPREINTEEARKNWQILEHTNLKRISLGDSTELSGYDFGQFDIIVDGMLGTGIAGEPREPYKSTILKINETRAKKVCIDIPTGYGSGVAIDSDVTLSFHFPKCDGAEVLPLGIPPDIEQCIGPGDVKILKRRTADAHKGQAGRVMVVGGSSFFRGALEYAGKAAAKIVDLVYHCSPQPCSGAVEKVPDFLGTCLDGEFLTEAHLPEIIKRVNHYRCDSILIGPGLGLGPEVGIKGETKKMVIGLVRELGGKKMVIDADGLNALLGNLDILHENVVLTPHRGEFKRLSGQEPAPENVTNFADKYGCIVVLKGPVDVVSNGMRTKFNHTGNPGMATGGTGDVLSGAIAGFAAQNDLFEACVAATFVTGLSGDFVREEKGYHFTATDVLEGLSEAIRWAEEF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration The designed protein sequence is ",
    "response#1": "HKYTHGHSLILSGPPGHGGAARLSARGALRIGAGLVTLGCPPAAVAENAARLDAVMLREVADAGALAAVLEDRRINALCLGPGLGISDETRALTAQGLKSGRAAVLDADALTLIAGDAALFAALHEGCVLTPHGGEFARLFPDLAEKLAAPASKGPAYSKVDAAREAAARAGAVVLFKGPDTVIAGPSGRCAVNAAVYERAAPWLATAGAGDVLAGFIAGLLARGFSAFDAACTGAWLHVECARSFGPGLIAEDLPEELY",
    "response#2": "MLLPRARDSHKGLSGKVTVVGGDKGMAGAIKLAAEACLRAGTGLVTVLSRPEHLNIINANRPELMFWGCEVVDMEVYLRLGWANTLIIGPGLGKQSWGYNLFKAVRLSDKPCVVDADALNLLSEEHCNNNNWVLTPHSGEAARLLGITIAEVERDRFAAADNIQKRYGGVVVLKGPGSLIFDGKKCVVAPVGNPGLASGGCGDVLTGVIAALISQGFSLFDAAIMGVIVHGEAADRAVTAGERGMLASDLMPHIRSLVADAHKYRRGLLGIVGGEMPGAALLSAAAAQNAGAGYVKLATASPLHQADPALVLTTDVSATLADKRTAALLVGPGLGRSDAASRLLAQALAAERPTVIDADALVLLRSAMLTHAPCILTPHDGEMLALERSFALESTGLRRDRARALATATGTVVVLKGPDSLIAAPDGSLILSPRASPWLSVAGTGDVLAGTIASRLAVHADPLRAAQEGLWLHGEAARLCGAAFTPTELAHAIRLALRTAHKWSAGAVLVVGGSAGMTGAATMAARAALEFGAGAVYLATPEAGVAHSVVPQIPAFPLDAVPRPERFDVVVAGPGLAAADLDAALPLVEGAKRVVLDAGALVPAVVEAATNAGAEVVVTPHAAEFERIAGVGVGTFSIRSYALRTGVVVLAKGNPTRVTDGGLPVFVRSGTQALASIGTGDVLAGMLGALWARGLGAMEAAVSAAYWHGRVAAGLSRVGTVTAR",
    "response#3": "GVVAGSERYPGAAVLACRGALAAGAGMVRYLGPPDVADLVRESCPEVVCGQGSSAEAHVQAWVLGPGVDESAGDQLQRIRDAAATGLPVVADAGALPALPRILAPHQILTPHIGELAALLAGYGVESDRARLEGATLAAARRGAALTGATVLLKGATTLVAAPGGTVFSQSEATPWMATAGSGDVLAGVLGALLAQLSGSATMDHVPRGDRWAAIAAMAASLHGRAGAMASAGGPVTAV"
  },
  {
    "reference": "MRLHELVTDLPDVRFTEDNPEVLGLATDSRKVRSGDLFVAIRGGEEQDRHPYVKDAIAAGAVAAVVEEVVESHSAPLVEVPSTRQALSILSKRFYGSPDSALKLVGITGTNGKTTTAYMTYAMLAAVDMNPGLVGTVEYLIGDRSAHSSNSTPEANELQSMFREMVEAGCQSAVIEATSHGLALGRVGGMEFDVGVFTNLTRDHLDFHRSQEAYLSAKALLFDNLKTDAYAIVNVDDTAHSEILARCRSKVLRYGYSASADIRIMEGQTDWRGVRIELKTPEGPQEIESILHGQFNFWNIAAVVATGMALGVSKEVTAQAVREVKVPGRFETVNRGQLFGVVVDYAHTPDGLENVLQTAKDLTDGRLICVFGCGGDRDRGKRPEMGRVAAKFADLSFVTSDNPRTENPGSIVNEIIPGLGSAPHFVEVNRRKAIEGAIHSANPGDLVLIAGKGHEDYQEIGRERIHFDDREVAREVLEMPSP",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be able to bind ATP ligand in a variety of conditions2. The protein should be designed to catalyze the following reaction ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.3. The designed protein should contain at least one Meso-diaminopimelate recognition motif motif to achieve its intended function.4. The protein design should take into account the physiological conditions of the Cell wall biogenesis pathway.5. A protein with magnesium ion binding functions is required to enable cell cycle, peptidoglycan biosynthetic process, cell division.6. The designed protein have Mur ligase N-terminal catalytic domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MKLGELLKNLPELKQISGDMENLEITSIENDNRKVRQGGLFVCIKGYTVDGHDFAESAEKNGAAAVLAERELSLNIPVIPDPRELAATLSSRFYNNPSKNLKLIGITGTNGKTTTTYLVKSMFEEAGCPTGVIGTIQNVIGREIVPAKHTTPESSDLAELLDRMVRSGMKAVAMEVSSHAIALDRISGLEFDMGVFTNITQDHLDFHKTFDNYVAAKAKFFKGLGADSHENVKKCPKVAVVNIDDPNTPRIVEGTPAEVVTYGVRNEADIRALNVRLGLTGISFEAATPAGTVALNMKLKGMFNVYNALAAVGLASEMGIDYRLIREGLSKFKGVEGRVEMVKVSDDFILLIDYAHNAMALSSILTTLREYHPGRLVCVFGCGGNRARSRRYEMGEVSGRMADFTIITSDNPRYENPEAIMDDIEVGMKKTTGKYIRIADRKEAIAYAIHHGKPGDIIVLAGKGHETYQIIGKEKYHFDDKEEAEKALKKRGY",
    "response#2": "MKRQLATDSRAVTPGDTFVAYPGNRADGRQLINQAIKNGANAVIWEAQGFNWNESWNIPNRAVANLRQQAGEIANHVDIYFSELKNNHKNIIGITGTNGKTTTAAAIYSTLLDLNKSVFLCGTRGAFVNEKVVDEKSLTTSPPLKMLSYLQTATKEKCEYFIMEVSSHAIAQNRIEGLNFALKVFTNLSQDHLDYHKTLQEYYAVKSSFFMDESLKLINKDDENIQFNYKNAYTYSLNNPATFSTLAYSLNNGIDAILRTMDAEAEISSSMVGEFNLYNLIAAFCLSSGIGFKKILENIENLKGVEGRIEKINNKKDLNIFVDYAHTPDALKNVLSALKKSCTGKLITIIGCGGERDKSKRPLMTEEALKLSDKIIITDDNPRNEDPQQIRKDMTSKTPKKKLVYIKEIPSRKKAIQYCINVLEKNDFLLIAGKGHEKYQIIGTKKIHFDDREVARKYLK",
    "response#3": "MKLRDLLSDLQVIELIGDESCVTGFAIDSRKVAPGTVFGAFRGVVQNGEDYIPAAIAAGAVAIVARPEAIVDGAIHIAGITGTNGKTTTAAAIYSTLLDLNKSVFLCGTRGAFVNEKVVDEKSLTTSPPLKMLSYLQTATKEKCEYFIMEVSSHAIAQNRIEGLNFALKVFTNLSQDHLDYHKTLQEYYAVKSSFFMDESLKLINKDDENIQFNYKNAYTYSLNNPATFSTLAYSLNNGIDAILRTMDAEAEISSSMVGEFNLYNLIAAFCAAALCGAELNTIGTALSEYSGAEGRVEMVKVSDDFILLIDYAHNAMALSSILTTLREYHPGRLVCVFGCGGNRARSRRYEMGEVSGRMADFTIITSDNPRYENPEAIMDDIEVGMKKTTGKYIRIADRKEAIAYAIHHGKPGDIIVLAGKGHETYQIIGTTKVHFDDREVAAEALRARGLL"
  },
  {
    "reference": "MRHISSEEMNSLDMNCKYFGLLPLQLMENAGAALAREIKATATGERIAIVAGRGNNGGDALVAARHLDGFRVTVYLLGRSGDIATEEAKRNWDILEHLGFDLREVRDPSDLSLGEYDLVLDAIFGTGVHGTVKGLEAGAIDAINSAGKQVISVDIPSGLGTNKAVRADTVVTFHRPKHGMTGIVKVVDIGIPPEAEFFAGPGDLWLVGRRAAESRKGDSGRILVIGGGPYTGAPALTAMAALRAGADVVTVATPKSASSTIAGFSPNLIVRELSGDHLRPKDMSVLKDLIARHDVVVMGMGLGRDQETLEALAQIIPICGRTVIDADALMPDLPLKGIVTPHVGEFKRISGIDLYGSGYRERIDPLKKYAREKGLVVLLKGRVGLVSNLITDGEIIRANANGNPGMTVGGTGDVLAGVTAAFFARTTALRAATAAAFVNGRAGDMVYAEKDFGMVATDVIEKIPLAMRL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have ADP-dependent NAD(P)H-hydrate dehydratase activity, kinase activity, metal ion binding to facilitate phosphorylation, nicotinamide nucleotide metabolic process.2. The (6S)-NADPHX, K(+) binding site should be located in a region of the protein that is accessible to the ligand.3. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration The designed protein sequence is ",
    "response#1": "MGRLDARATEEYGIPSLILMENAGIQVAREVSRLLAGNIDGKRVLILCGKGNNGGDGFVAARHLINNGAEVILFLLASERDIKGDALTNLTIYQRMGGKVYSFQDPKDLNALRLALLSASVVVDAIYGTGFRGDVPELIAKAMTMINESDLPVLAVDLPSGLEADTGKIPSACLQAKVTVTLGLPKVGLVVEPGASAAGEVVVVDISIPGEMIEAHKYSRGHAVVFSGGVHATGAARLSALAAARSGAGAVTLLSPPDAMAVNAAHLTSIMVRETRSLKDVRQFVSDRKVAAAVLGPGYGNPAFAREHALHLASGGVEGLHGLVLDADGITAFEHDPMQLFDARRETDVALVLTPHEGEFRRLFPDIASRELSKLEKAREAAGRANAVVIYKGPDTVIAEPGGLAVINANGTPLLATAGSGDVLAGIVCGLLAQRMPAFAAACAAVWTHADAARRFGHGLIADDLPLQACLAPRRRNSHKGSFGDVAVLGGAPGMLGAGLLAARGALFAGAGRVFLAALDAALGVDPRQPEIMLRDAAGFAFERRTVVAGPGLGDSNQAMRLLGKLIDGSGPLLLDADGLNLVAASPDLQARLAHHDGPVVLTPHPLEAARLLGVTAAIVQADRLENARELAQRLNAVVVLKGAGSVIARQNGEVAINPTGNPGLATGGSGDVLAGVCGALLGQGWPAWEAALGAVWLHGAAADRLVVDGAGPIGLTAGELPRAIRAELALLPVRTASAHKGTAGRVWIVGGHPGTVGAPALAGLGALRAGAGLVTVHSAADVPLVTPELMAQRHADLRTFLTSTGLTGTGARPDAVAIGMGLGPEAPALAREVLDWEIPTVLDADALQPDLCGYGHDRCVWTPHPGEAARVLGVPTPEVTRDPLSAARALQDRLGGVVVLKGGPSVVARAGDLSVARGGHPGMASAGMGDTLCGILAALLAQGLDPWDAARAGVRLHARAGEIAAVRFGYGLGATDVAHDLGRA",
    "response#2": "SHKGENGSVLIIGSNSDYIGAVVFAAESALKMGVDLVYIVAPSISADVIKSYNPNFIVKTVEGNLLKEEHFDEIMDLVDRVDSVLIGSGAGLSEDTGKLFNKLVQSIDKVMVLDADALKLVDKENIIGSKTIVTPHTREFETFFQKELPENEDKKIELLSSLSREYNTVITLKGVYDIITSPEDYKLNSTGNQGMTVGGTGDVLAGITVALTCKCDVFTAAYLATYIIGTAADNLFKSHGFNYTSKDLLDLE",
    "response#3": "MREIDNHTIEVLGIPGIVLMENAARGVCEVIYDRVKGSSALVVCGKGNNGGDGLAVARNLYNMGYDVEVILTAPVEELKGDAKKNAEILSALPVPVHVVDSTEKLFELYTFAKECDFIVDGLFGTGLSKPLSGFYAELVDFINRLNKTVISIDIPSGLSADTGQVIGPHVKADYTVTFAFPKVAHVMPPACYSVGEVFVVDISIPEDVPE"
  },
  {
    "reference": "MGRNGANHSIQTVSEYEPIGEDEERTPLLPQGVQTRGSSFMSSAFNLMNAIMGSGILGLSYAMANTGTVGFSILLLMVASLAAYSIHLLLLLCDKTGINSYEALGEKALNRPGKILVACTILIQNIGAMSSYLFILKTELPAAIIGFMRSDSETSGKWFENGVTLLILVTVIIVLPLALLPKIGFLGYTSSIAFLFMLFFTVVVVVKKWSIPCPLPINSTLSLSLNTSECTAQLFVISSKSAYAVPTMAFSFLCHTAVFPIYCELHRPTKRRMQRATNVSIFLSFVVYLISALFGYLTFYSHVGSELLLAYNTYLPRDILVMSVRLAILLAVLLTVPLIHFPARKAVLMLCRGEREFSWLSHTLSCFFILTLVLLLAIFVPDIKNVFGVVGSTTSTCLLFVYPGMFFLRISSEPIRSFNSVGAVFLLVIGLVVGVLSLSVIIVSWVQGP",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that can perform L-amino acid transmembrane transporter activity in plasma membrane to support amino acid transmembrane transport, glutamine transport.2. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "MTWFQTLIHLLKGNIGTGLLGLPLAVKNAGLLLGPLSLLVIGIVAVHCMGILVKCAHHLCRRLNKPFLDYGDTVMYGLECSPSTWIRNHSHWGRRIVDFFLVVTQLGFCCVYFVFLADNFKQVIEAANGTTTNCNNNETVILTPTMDSRLYMLTFLPFLVLLSFIRNLRILSIFSLLANISMFVSLIMIYQFIVQRIPDPSHLPLVAPWKTYPLFFGTAIFAFEGIGVVLPLENKMKDSQKFPLILYLGMAIITVLYISLGSLGYLQFGADIKGSITLNLPNCWLYQSVKLLYSIGIFFTYALQFYVAAEIIIPAIVSRVPERFELVVDLSARTAMVCVTCVLAVLIPRLDLVISLVGSVSSSALALIIPPLLEVTTYYGEGISPLTITKDALISILGFVGFVVGTYLYTKQ",
    "response#2": "MTWFQTLIHLLKGNIGTGLLGLPLAVKNAGLLLGPLSLLVIGIVAVHCMGILVKCAHHLCRRLNKPFLDYGDTVMYGLECSPSTWIRNHSHWGRRIVDFFLVVTQLGFCCVYFVFLADNFKQVIEAANGTTTNCNNNETVILTPTMDSRLYMLTFLPFLVLLSFIRNLRILSIFSLLANISMFVSLIMIYQFIVQRIPDPSHLPLVAPWKTYPLFFGTAIFAFEGIGVVLPLENKMKDSQKFPLILYLGMAIITVLYISLGSLGYLQFGADIKGSITLNLPNCWLYQSVKLLYSIGIFFTYALQFYVAAEIIIPAIVSRVPERFELVVDLSARTAMVCVTCVLAVLIPRLDLVISLVGSVSSSALALIIPPLLEVTTYYGEGISPLTITKDALISILGFVGFVVGTYL",
    "response#3": "MTWFQTLIHLLKGNIGTGLLGLPLAVKNAGLLLGPLSLLVIGIVAVHCMGILVKCAHHLCRRLNKPFLDYGDTVMYGLECSPSTWIRNHSHWGRRIVDFFLVVTQLGFCCVYFVFLADNFKQVIEAANGTTTNCNNNETVILTPTMDSRLYMLTFLPFLVLLSFIRNLRILSIFSLLANISMFVSLIMIYQFIVQRIPDPSHLPLVAPWKTYPLFFGTAIFAFEGIGVVLPLENKMKDSQKFPLILYLGMAIITVLYISLGSLGYLQFGADIKGSITLNLPNCWLYQSVKLLYSIGIFFTYALQFYVAAEIIIPAIVSRVPERFELVVDLSARTAMVCVTCVLAVLIPRLDLVISLVGSVSSSALALIIPPLLEVTTYYGEGISPLTITKDALISILGFVGFVVGTYLFI"
  },
  {
    "reference": "MAETLQLNSTFLHPNFFILTGFPGLGSAQTWLTLVFGPIYLLALLGNGALPAVVWIDSTLHQPMFLLLAILAATDLGLATSIAPGLLAVLWLGPRSVPYAVCLVQMFFVHALTAMESGVLLAMACDRAAAIGRPLHYPVLVTKACVGYAALALALKAVAIVVPFPLLVAKFEHFQAKTIGHTYCAHMAVVELVVGNTQATNLYGLALSLAISGMDILGITGSYGLIAHAVLQLPTREAHAKAFGTCSSHICVILAFYIPGLFSYLTHRFGHHTVPKPVHILLSNIYLLLPPALNPLIYGARTKQIRDRLLETFTFRKSPL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have G protein-coupled receptor activity and be active in plasma membrane.2. The protein should have Helical; Name=7, Helical; Name=5, Helical; Name=2, Helical; Name=4, Helical; Name=1, Helical; Name=3 domains to ensure proper insertion into the membrane.3. The Extracellular, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "GNGLVIWIFCAAKSLRTPSNMFVVNLAICDFFMMIKTPIFIYNSFNTGFALGNLGCQIFAVIGSLTGIGAAITNAAIAYDRYSTIARPLDGKLSRGQVILFIVLIWTYTIPWALMPVMGVWGRFVPEGFLTSCSFDYLTDTNEIRIFVATIFTFSYCIPMILIIYYYSQIVSHVVNHEKALREQAKKMNVDSLRSNANTSSQSAEIRIAKAAITICFLYVLSWTPYGVMSMIGAFGNKALLTPGVTMIPACTCKAVACLDPYVYAFGHPKYRLAFKRSGAWGIVLEAVAGAGIVTTFVLTIILVASLPFVQDTKKRSLLGTQVFFLLGTLGLFCLVFACVVKPDFSTCASRRFLFGVLFAICFSCLAAHVFALNFLARKNHGPRGWVIFTVALLLTLVEVIINTEWLIITLVRGSGEGGPQGNSSAGWAVASPCAIANMDFVMALIYVMLLLLGAFLGAWPALCGRYKRWRKHGVFVLLTTATSVAIWVVWIVMYTYGNKQHNSPTWDDPTLAIALAANAWAFVLFYVIPRSGAWGIVLEAVAGAGIVTTFVLTIILVASLPFVQDTKKRSLLGTQVFFLLGTLGLFCLVFACVVKPDFSTCASRRFLFGVLFAICFSCLAAHVFALNFLARKNHGPRGWVIFTVALLLTLVEVIINTEWLIITLVRGSGEGGPQGNSSAGWAVASPCAIANMDFVMALIYVMLLLLGAFLGAWPALCGRYKRWRKHGVFVLLTTATSVAIWVVWIVMYTYGNKQHNSPTWDDPTLAIALAANAWAFVLFYVIP",
    "response#2": "RSGAWGIVLEAVAGAGIVTTFVLTIILVASLPFVQDTKKRSLLGTQVFFLLGTLGLFCLVFACVVKPDFSTCASRRFLFGVLFAICFSCLAAHVFALNFLARKNHGPRGWVIFTVALLLTLVEVIINTEWLIITLVRGSGEGGPQGNSSAGWAVASPCAIANMDFVMALIYVMLLLLGAFLGAWPALCGRYKRWRKHGVFVLLTTATSVAIWVVWIVMYTYGNKQHNSPTWDDPTLAIALAANAWAFVLFYVIPS",
    "response#3": "GNGLVIWIFCAAKSLRTPSNMFVVNLAICDFFMMIKTPIFIYNSFNTGFALGNLGCQIFAVIGSLTGIGAAITNAAIAYDRYSTIARPLDGKLSRGQVILFIVLIWTYTIPWALMPVMGVWGRFVPEGFLTSCSFDYLTDTNEIRIFVATIFTFSYCIPMILIIYYYSQIVSHVVNHEKALREQAKKMNVDSLRSNANTSSQSAEIRIAKAAITICFLYVLSWTPYGVMSMIGAFGNKALLTPGVTMIPACTCKAVACLDPYVLFCLVNPLFRKAYYNALLVLANLHSKASMTMPDVYFVNMAVAGLVLSALAPVHLLGPPSSRWALWSVGGEVHVALQIPFNVSSLVAMYSTALLSLDHYIERALPRTYMASVYNTRHVCGFVWGGALLTSFSSLLFYICSHVSTRALECAKMQNAEAADATLVFIGYVVPALATLYALVLLSRVRREDTPLDRDTGRLEPSAHRLLVATVCTQFGLWTPHYLILLGHTVIISRGKPVDAHYLGLLHFVKDFSKLLAFSSSFVTPLLYNALLVLANLHSKASMTMPDVYFVNMAVAGLVLSALAPVHLLGPPSSRWALWSVGGEVHVALQIPFNVSSLVAMYSTALLSLDHYIERALPRTYMASVYNTRHVCGFVWGGALLTSFSSLLFYICSHVSTRALECAKMQNAEAADATLVFIGYVVPALATLYALVLLSRVRREDTPLDRDTGRLEPSAHRLLVATVCTQFGLWTPHYLILLGHTVIISRGKPVDAHYLGLLHFVKDFSKLLAFSSSFVTPLL"
  },
  {
    "reference": "MAWNTNLRWRLPLTCLLLQVIMVILFGVFVRYDPDADAHWIDERLGRNISSDMDNEFYYRYPSFQDVHVMIFVGFGFLMTFLQRYGFSSVGFNFLLAAFGIQWALLMQGWLHSFHSGYIVLGVENLINADFCVGSVCVAFGAVLGKVSPVQLLIMTLFQVTLFSVNEFILLNLLEVKDAGGSMTIHTFGAYFGLTVTWILYRPNLYQSKERQSSVYHSDLFAMIGTLFLWMYWPSFNSAVSHHGDAQHRAAINTYCSLAACVLTSVALSSALHKKGKLDMVHIQNATLAGGVAVGTAAEMMLMPYGSLIVGFICGIISTLGFVYLTPFLESRLRIQDTCGIHNLHGMPGIIGGIVGAVTAASANTQQYGQKGLAHAFDIDATKTTWTASMQGSFQAAGLFVSLAMALVGGLIVGVILKLPFWGQPADENCFEDAIYWEIPEDQKSLVSRSEDPTLRPTEP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that can perform ammonium transmembrane transporter activity, ankyrin binding in apical plasma membrane, plasma membrane, basolateral plasma membrane, cytoplasmic vesicle to support cellular ion homeostasis, ammonium transmembrane transport, transepithelial ammonium transport, ammonium homeostasis.2. For general function, the protein need meet that May regulate transepithelial ammonia secretion .; Functions as an electroneutral and bidirectional ammonium transporter3. The Extracellular, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.4. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "AWQLVAATFVGIQSMPGLVVIYGSIVKKKWAVNSAFMALYAYASTLIVWVLVGFRMAFGDRLLPFWAKAGPALTQDFLVQRAVFPATAHYGSDGTLETPRTEPFYAEAALVLFEFEFAAITLVLLAGSLLGRMNIKAWMAFTPLWLLFSYTVGAFSLWGGGFLYQWGVIDYSGGYVIHLSSGVAGFTAAYWVGPRLKSDRERFSPNNILLMIAGGGLLWLGWAGFNGGAPYAPNVTATVAVLNTNVSAATSLLTWTCLDVIFFGKPSVIGAVQGMMTGLVCITPGAGLVHTWSAMLMGMFAGSVPWFTMMILHKKSTFLMKVDDTLAVFHTHAVAGILGGVLTGLLATPELCALDCPIPNMRGVFYGSGIGQLGKQLGGALFVTVWNLIVTSAILLCIGLFIPLRMSDDQLMIGDDAAHGEEAL",
    "response#2": "YLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIGKHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVYPVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIGRFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAVGRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAIICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQPGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGMDQTRHGGFAVFMFFCALLVWLMTPGLALFYGGMVKSKNVLSTAMHSFSSIAIVSIVWVLFGYTLAFAPGNSIIGGLEWAGLKGVGFDPGDYSDTIPHSLFMMFQMTFAVLTTAIISGAFAERMRFGAFLLFSVLWASLVYTPVAHWVWGGGWIGQLGALDFAGGNVVHISSGVAGLVLAIVLGKRKDGTASSPHNLIYTFLGGALIWFGWFGFNVGSALTLDGVAMYAFINTNTAAAAGIAGWILVEWIINKKPTMLGAVSGAIAGLVAITPAAGFVTPFASIIIGIIGGAVCFWGVFSLKKKFGYDDALDAFGLHGIGGTWGGIATGLFATTSVNSAGADGLFYGDASLIWKQIVAIAATYVFVFIVTFVIIKIVSLFLPLRATEEEESLGLDLTMHGEKA",
    "response#3": "AWQMISATLVGMQSVPGLVILYGSIVKKKWAVNSAFMALYAFAAVWLCWVTWGYNMSFGHKLLPFWGKARPALGQSFLLAQAVLPQTTQFYKGGGGADAVVETPWVNPLYPMATMVYFQCVFAAITLILLAGSLLGRMNIKAWMLFVPLWLTFSYTVGAFSLWGGGFLFHWGVMDYSGGYVIHLSSGVAGFTAAYWVGPRSTKDRERFPPNNVLLMLTGAGILWMGWAGFNGGDPYSANIDSSLAVLNTNICAATSLLVWTCLDVIFFKKPSVIGAVQGMITGLVCITPGAGLVQGWAAIVMGILSGSIPWFTMMVVHKRSRLLQQVDDTLGVFHTHAVAGFLGGATTGLFAEPVLCSLFLPVTNSRGAFYPGRGGGLQFVRQVAGALFIICWNVVVTSLVCLAVRAVVPLRMPEEELAIGDDAVHGEEAL"
  },
  {
    "reference": "MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIARGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEMKEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFVFEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIVDSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEILALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNTQPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRMHSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFEKQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGRYADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGISPTKPLTAAAVKTPS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that contributes to peptidoglycan biosynthetic process, proteolysis by functioning in plasma membrane with serine-type D-Ala-D-Ala carboxypeptidase activity, penicillin binding.2. For general function, the protein need meet that Catalyzes cross-linking of the peptidoglycan cell wall at the division septum. The designed protein sequence is ",
    "response#1": "GTVVVLDARTGEILALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNTQPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRMHSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFEKQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGRYADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIR",
    "response#2": "GTVVVLDARTGEILALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNTQPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRMHSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFEKQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGRYADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIL",
    "response#3": "GTVVVLDARTGEILALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNTQPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRMHSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFEKQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGRYADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIYRGAENYIDLSKSEDVEETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVALDRRALGFAARAVDNEKMGEDRIRRGAVIRVKNNGGRWAVVQEC"
  },
  {
    "reference": "MSSYSTRQVGAANTLDYKVYIEKDGKLVSPFHDIPLYANEEKTILNMIVEVPRWTNAKLEISKEQKLNPIIQDTKKGKLRFVRNCFPHHGYIHNYGAFPQTWEDPNQSHPETKAKGDNDPLDVCEIGEKVATVGEVKQVKVLGVMALLDEGETDWKVIVIDVNDPLAPKLNDIEDVETHLPGLLRATNEWFRIYKIPDGKPENQFAFSGECKNKKYAEEVIGECAEAWEKLIKGESVDSKGIDLTNTTLSSTPSYSDAAAQEIPSASPAPAAPIDKSIDKWFFISGAH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein requires Mg(2+) to activate a critical catalytic site.2. The desired protein should be able to perform diphosphate + H2O = H(+) + 2 phosphate3. A protein that localizes to fungal biofilm matrix, cytosol and can perform inorganic diphosphate phosphatase activity with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "YSDLGQTYASNQTLYNYMSNPKGQAVLFVGDLSYADDHPNHDQRKWDSYGRFVEPSAAYQPWSWAAGNYEIDYAQSISETQPFKPYKNRYHVPYKASQSTSPLWYSIKRASTYIIVLSSYSAYDKYTPQNSWLQDELKKVNRSETSWLIVLVHAPWYNSNNYHYMEGESMRVTFEPWFVENKVDIVFAGHVHAYSDLGQTYASNQTLYNYMSNPKGQAVLFVGDLSYADDHPNHDQRKWDSYGRFVEPSAAYQPWSWAAGNYEIDYAQSISETQPFKPYKNRYHVPYKASQSTSPLWYSIKRASTYIIVLSSYSAYDKYTPQNSWLQDELKKVNRSETSWLIVLVHAPWYNSNNYHYMEGESMRVTFEPWFVENKVDIVFAGHVHAYERSKRISNIHYNITDGMSTPVKDQNAPIYITIGDGGNIEGIANSFTDPQPSYSAFREASFGHALLEIKNRTHAHYTWHRNKEDEAVIADSIWLKR",
    "response#2": "YSDLGQTYASNQTLYNYMSNPKGQAVLFVGDLSYADDHPNHDQRKWDSYGRFVEPSAAYQPWSWAAGNYEIDYAQSISETQPFKPYKNRYHVPYKASQSTSPLWYSIKRASTYIIVLSSYSAYDKYTPQNSWLQDELKKVNRSETSWLIVLVHAPWYNSNNYHYMEGESMRVTFEPWFVENKVDIVFAGHVHAYSDLGQTYASNQTLYNYMSNPKGQAVLFVGDLSYADDHPNHDQRKWDSYGRFVEPSAAYQPWSWAAGNYEIDYAQSISETQPFKPYKNRYHVPYKASQSTSPLWYSIKRASTYIIVLSSYSAYDKYTPQNSWLQDELKKVNRSETSWLIVLVHAPWYNSNNYHYMEGESMRVTFEPWFVENKVDIVFAGHVHAYERSKRISNIHYNITDGMSTPVKDQNAPIYITIGDGGNIEGIANSFTDPQPSYSAFREASFGHALLEIKNRTHAHYTWHRNKEDEAVIADSIWLKR",
    "response#3": "YSDLGQTYASNQTLYNYMSNPKGQAVLFVGDLSYADDHPNHDQRKWDSYGRFVEPSAAYQPWSWAAGNYEIDYAQSISETQPFKPYKNRYHVPYKASQSTSPLWYSIKRASTYIIVLSSYSAYDKYTPQNSWLQDELKKVNRSETSWLIVLVHAPWYNSNNYHYMEGESMRVTFEPWFVENKVDIVFAGHVHAYSDLGQTYASNQTLYNYMSNPKGQAVLFVGDLSYADDHPNHDQRKWDSYGRFVEPSAAYQPWSWAAGNYEIDYAQSISETQPFKPYKNRYHVPYKASQSTSPLWYSIKRASTYIIVLSSYSAYDKYTPQNSWLQDELKKVNRSETSWLIVLVHAPWYNSNNYHYMEGESMRVTFEPWFVENKVDIVFAGHVHAYERSKRISNIHYNITDGMSTPVKDQNAPIYITIGDGGNIEGIANSFTDPQPSYSAFREASFGHALLEIKNRTHAHYTWHRNKEDEAVIADSIWLKH"
  },
  {
    "reference": "MTRFVFITGGVVSSLGKGIAAAALGALLQARGYSVRLRKLDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGVHATRADNATTGRLYAEVIARERRGDYLGATVQVIPHITDAIKETIVRDTEGLDFVLIEIGGTVGDIESLPFLEAIRQLGNELGHERAMFVHLTLVPYIPSAGELKTKPTQHSVKELLNVGIQPQLLICRCDRPIPPNERRKIALFCNVRPEAVIAALDVDTIYAVPVSYSAEGLDREVLRHFDLPHATPPDLSRWERVAATVRAPEGEVKIAVVGKYTSLLDSYKSLAEAMAHGGIAHRVRVLPEWVDSEVFSQPDRISRLEGVHGILVPGGFGERGTLGMIEAVRFARERRVPFLGICFGMQMAVIEAARNLAGLPGASSTEFGPCDVPVVGLLTEWARGNEIERRREGGNLGGTMRLGAYAASLAEGSLVSEIYGGATRIEERHRHRYEVNIHYRTALEATGLRFSGLSPDGVLPEIIELPSHPWFVGVQFHPELKSKPFEPHPLFAGFIGAAVRQARLV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile; for glutamine hydrolysis, Nucleophile to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).2. The protein design should take into account the physiological conditions of the CTP biosynthesis via de novo pathway, CTP from UDP: step 2/2, Pyrimidine metabolism pathway.3. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP, L-glutamine, UTP, CTP, Mg(2+) binding.4. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.5. The designed protein must possess metal ion binding, ATP binding, glutaminase activity, CTP synthase activity to support 'de novo' CTP biosynthetic process, glutamine metabolic process under certain conditions.6. The designed protein have Glutamine amidotransferase, CTP synthase N-terminal domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MFITGGVVSSLGKGTASASLGAILEARQLKVTLLKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFVRTTMGQHNNFTTGRIYENVIRKERRGDYLGATVQVIPHIVDEIKQCLLNNTGDADVAMVEIGGTVGDIESLPFLEAIRELGRELGHERTLFIHLTLVPYIPSVGELKTKPTQHSVKELRSIGIQPDILLCRSTRPLPEGERQKISLFTSVDEQAVISVVDVDSIYRIPLLLHQQGLDKILCDKLKVKIAICGKYVGLKDSYKSIIEAFVHAGVSNHAQVELKWVDSEEIESQGVGDKFEDVAGILIPGGFGVRGIEGKVQAVKYARERKLPFFGICLGLQCAVIEFARNVCGLNAANSSEFDPKTPHPVVDLMKEQLDVEDKGGTMRLGAYACRVKKGTKTYEAYGQELIYERHRHRYEINNEYTEILEKHGLLAAGINPDKNLIEIMELTDHPWFIGVQFHPELKSRVLKAHPLFKNFIEATL",
    "response#2": "MFITGGVVSSLGKGTASASLGAILEARQLKVTLLKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFVRTTMGQHNNFTTGRIYENVIRKERRGDYLGATVQVIPHIVDEIKQCLLNNTGDADVAMVEIGGTVGDIESLPFLEAIRELGRELGHERTLFIHLTLVPYIPSVGELKTKPTQHSVKELRSIGIQPDILLCRSTRPLPEGERQKISLFTSVDEQAVISVVDVDSIYRIPLLLHQQGLDKILCDKLKLDPKPENLTEWKELVERIKKPKGDTESYKSLIEALNHAGMHTLSKVNIHYLDSEDIEKNGCGALKAMDAILVPGGFGRRGTEGKIAAIRYARENKVPYLGICLGMQLAVVEFARDVAGMNGAHSTEFDKHTDYPVIGLITEWLDASGKVETRGEDSDLGGSMRLGGQVCNLTDGTLARDIYGKPEITERHRHRYEVNNTLLAKLEEQGLRVAGRAPGTDLCEMIELPTEGPNAHPWFVGCQFHPEFTSNPRNGHPLFTAFVVNVAIVGKYTQLEDAYKSIKEALVHGGMANRVKVNVSWVDAEVFDKGDAAPHLEGFHAILVPGGFGERGTEGKIKAAQFARERKVPYLGICLGMQMAVIEAARNTAKIKHAGSEEFDHEAGGKRFEPVVYHLKEWVQGNYTVTRQAGDDKGGTMRLGAYDATLKEGSRVAEVYGTTSIEERHRHRYEVDVKYRDKLEACGLTFSGMSPDGRLPEIVEWSDHPWFIGVQFHPELKSKPFEPHPLFADFVGAAIDYKNKLKDAYLSLSEALCHGGIAHNTAVEIVWVDAEQVEQGDLGEIFSGVSGILVPGGFGGRGVAGKIRAINYARTRGVPFLGICMGMQCAVIEFARNVCGLKAANSTEFDPETPYPVIDLLPDQRNIEDLGGTMRLGLYPCRLLPGSRAAQAYGVEEVKERHRHRYEFNNRFRDLLEEKGLKVTGTSPDGVLVEVVEIPDHPWFLACQFHPEFKSRPTRPHPLFRAF",
    "response#3": "MFITGGVVSSLGKGTASASLGAILEARQLKVTLLKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFVRTTMGQHNNFTTGRIYENVIRKERRGDYLGATVQVIPHIVDEIKQCLLNNTGDADVAMVEIGGTVGDIESLPFLEAIRELGRELGHERTLFIHLTLVPYIPSVGELKTKPTQHSVKELRSIGIQPDILLCRSTRPLPEGERQKISLFTSVDEQAVISVVDVDSIYRIPLLLHQQGLDKILCDKLKLPDAYKSVNEALKHAGLKNRLSIKIQYVDSQDLEVKGTDVLQHLDAILVPGGFGERGVEGKILAAQYARENKVPYLGICLGMQVALIEYARNVAGLKGAHSSEFDAKSKYPVVGLITEWIDEDGKVETRDVDSDLGGTMRLGSQLCHLTKGSKVADLYGSVEIYERHRHRYEVNNTILPKITAKGLQVTGLSSDKKLVEIIENPAHPWFVAAQFHPEFTSTPRDGHPLFEGFVKRCS"
  },
  {
    "reference": "MIKVNEYFSGNVKSIAVQNSDGEQTVGVMKAGEYKFSTSKKEFMFVASGQMDVKLPGESEFKSFSNHGSFVVEANQSFDVIVKEDTIYICRYE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be optimized for thymidine phosphorylase activity.2. The protein should have a low Km value for phosphate + xanthosine = alpha-D-ribose 1-phosphate +   xanthine. The designed protein sequence is ",
    "response#1": "MXKXKXDXXXIN",
    "response#2": "GVAS",
    "response#3": "MXXXGX"
  },
  {
    "reference": "MNPKSSTPKIPRPKNAFILFRQHYHRILIDEWTAQGVEIPHNSNISKIIGTKWKGLQPEDKAHWENLAEKEKLEHERKYPEYKYKPVRKSKKKQLLLKEIEQQQQQQQKEQQQQKQSQPQLQQPFNNNIVLMKRAHSLSPSSSVSSSNSYQFQLNNDLKRLPIPSVNTSNYMVSRSLSGLPLTHDKTARDLPQLSSQLNSIPYYSAPHDPSTRHHYLNVAQAQPRANSTPQLPFISSIINNSSQTPVTTTTTSTTTATSSPGKFSSSPNSSVLENNRLNSINNSNQYLPPPLLPSLQDFQLDQYQQLKQMGPTYIVKPLSHTRNNLLSTTTPTHHHIPHIPNQNIPLHQIINSSNTEVTAKTSLVSPK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with HMG box capability for targeted gene regulation.2. A protein that contributes to regulation of transcription by RNA polymerase II, negative regulation of transcription by RNA polymerase II, cellular response to osmotic stress by functioning in nucleus, chromatin with RNA polymerase II cis-regulatory region sequence-specific DNA binding, DNA-binding transcription repressor activity, RNA polymerase II-specific, DNA-binding transcription factor activity, RNA polymerase II-specific, DNA binding, bending, sequence-specific DNA binding.3. For general function, the protein need meet that Transcription factor that represses the expression of HEM13, COX5B, ANB1, CYC7 or AAC3 (hypoxic function) The designed protein sequence is ",
    "response#1": "MSPGVSDDGHSMSPGHSSGAPGGADSPLPGQQSQMSGIGDDGAGVSGGVSVKSDEEDDRFPIGIREAVSQVLNGYDWTLVPMPVK",
    "response#2": "MSPGVSDDGHSMSPGHSSGAPGGADSPLPGQQSQMSGIGDDGAGVSGGVSVKSDEEDDRFPIGIREAVSQVLNGYDWTLVPMPVS",
    "response#3": "KTMREISLPYSSSPSHMGSFLRTPTPSAFYYKAHGGSPVCTPLGQLSPPPEAPALDAMDHLNQAELWGDFDRNEFDQYLNMSRTQRPGYSFPMSKLGAPRTIPCEENSLISALSDASTAMYYTPCIP"
  },
  {
    "reference": "MRMLVSGRRVKKWQLIIQLFATCFLASLMFFWEPIDNHIVSHMKSYSYRYLINSYDFVNDTLSLKHTSAGPRYQYLINHKEKCQAQDVLLLLFVKTAPENYDRRSGIRRTWGNENYVRSQLNANIKTLFALGTPNPLEGEELQRKLAWEDQRYNDIIQQDFVDSFYNLTLKLLMQFSWANTYCPHAKFLMTADDDIFIHMPNLIEYLQSLEQIGVQDFWIGRVHRGAPPIRDKSSKYYVSYEMYQWPAYPDYTAGAAYVISGDVAAKVYEASQTLNSSLYIDDVFMGLCANKIGIVPQDHVFFSGEGKTPYHPCIYEKMMTSHGHLEDLQDLWKNATDPKVKTISKGFFGQIYCRLMKIILLCKISYVDTYPCRAAFI",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a low Km value for a neolactoside nLc4Cer(d18:1(4E)) + UDP-N-acetyl-alpha-D-   glucosamine = a neolactoside IV(3)-beta-GlcNAc-nLc4Cer(d18:1(4E)) +   H(+) + UDP.2. The protein must exhibit the following characteristics: Probably plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development.; Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures; Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc(4)Cer; paragloboside), resulting in the synthesis of Lc(3)Cer and neolactopentaosylceramide (nLc(5)Cer), respectively.3. The Lumenal topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells. The designed protein sequence is ",
    "response#1": "MDRWGGGGGGGGGGPRGGGGGRGGGGGGGGGGGGGGGGGGGRGGGGFGGGGGAGGGGGGAGGGRGGGGGGGGGGGGGGGGGGGRGGGGGAGGGGGGGGGGGRGGGGGRGGGGGRGGGGGGGGGGGGGGGGGRGGGGRGGGGGGGGGGGRGGGGGRGGGGGGAGGGGGGGGGGGRGGGGRGGGRGGGGGRGGGGGRGGGGGRGGGGGRGGGGGRGGGGGRGGGGGRGGGGGRGGGGGRGGGGGRGRGRGGGGGRGGGGGGGGRGRGGGGGRGGGGGRGGGGGRGGGGRGRGRR",
    "response#2": "MSQSPNGPPFGQMPAWKRMMAKQQQQEQQQQEQQQQEQQQQEEQQQQEEQQQQEEQQQQEQQQQEEQQQQEQQQQEQQQQEEQQQQEQQQQEEQQQQEQQQQEEQQQQEQQQQEEQQQQEEQKQEEQQQVEEGNQEANHKTEKEEEPMSEKPEMPAWKKMMAKHQQQEQQQQEQQQQEEQQQEEPQQPEQQPAAPEMPEWKKMMAQKQQQEQQQQEQQQQEQQQQEEQQQQEEQQQQEEQPQQEEQQPQEQPTEEQPTEEQPVEEQPTEEQPVEEQPTEEQPAEEQPAEEQPAEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPAEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQPTEEQP",
    "response#3": "MQCLRPLVHRPLARSAFSLSRPAAIHPAVRHLVSRPSQSRAFSSASPARHSSPSSSSSSSSPSEQGPRKQPRRPILSRVKTFWYRLSTGQRLYLVGLGSLAGVGFISTFEVPIRAKAQELDSHIPRWVMAIPFLRPLSNLPYYVAVPLYTGLGMTLASEVFKRPYLTRLPGFSRFFRRFSNGPLSAALGDPTAVYFGSIGMALILFKEVPALHRLAGYHPSYIDTSLALLSISVLHGFAGYLVLAPRVLHALRSAYLPPSLAASPATSTPTAPATAAASSQTSPTTTTPTTTAPAATPEAPAPWTSVRTWTALSRASRIRTPVTALWSAAMLASTAHHFIYPARMSAWMHAHSGAAEVGAGALARAAVWRGLARADVWAAGAVGLASVLLGVRVLRGVRAPRWVGKVGGFVGGKRGVLGAVVGVGGVWAMLWHSDRVQGMFGRYVPGWVDVVVRG"
  },
  {
    "reference": "MTKRRTVALIVAAGSGTRAGGTLPKQFATVAGRPMLMHAVTAMSAHPAIDAVTVVVAEGQLDHAKAVLAAGGVTDATVIVGGSTRRDSVLRGIAALSELDHVLVHDAARPFLPAGVISAVVAALANSEAAVPVLPVADTLARGATTLTGRMLGEVVPREAVYRVQTPQGFRIAKLRAAHAAWPADEDASDDAQMVRRVGGSVALVQGDPMLDKVTHPDDFAAAEARAPYESRTAMGFDVHRLETGEELWLGGVRIPHDKGLSGHSDADVVLHALTDALLGTIAAGDIGTHFPPSDPQWKGADSAQFLQHAAQLIAAKGGRIDFVDLTILCEMPKIGPHRAAMTNRIADLLDLTTDRVSVKATTTERLGFTGRGEGIAAQAVATISLPSNRGRGGA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be optimized for 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity, metal ion binding activity in order to improve terpenoid biosynthetic process, isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway.2. a divalent metal cation is required for the proper folding and stability of the protein.3. The protein must exhibit the following characteristics: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D- erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C- methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF)..4. The protein design should consider the endogenous proteins and enzymes present in the isopentenyl diphosphate biosynthesis via DXP pathway, isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6 pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "IRTGNGFDVHAFGPGDHVTLCGIDIPHTHGLVGHSDADVALHTVTDAIYGALARGDIGQHFPPSDPQWKGARSDIFLRHAAQLARDMGFTLTHVDCTLICETPKIGPHVQPMRESMADMLGIELDRVSVKATTSERLGFTGRGEGIACMATATLVS",
    "response#2": "RTGTGFNVHALAEGEELWLGGCKIDHDKGLAGHSDADVALHALVDAILGAIGNGDIGTHFPPGDDRWKGASSGQFLQHAVSLASEAGYRIGNLDVTIICEAPKIGPHKDTMREKIAELAGIQIAAVSVKATTTEKLGFTGRGEGIAAQAAATGVLIHDAAGEPMRTGTGFDVHRFGDGDHVWLCGVRIAHDRSLVGHSDADVGLHALTDAVLGAIADGDIGQHFPPSDPQWAGAASHIFLREAARRVRQRDGRIVNVDVTILCEAPKIAPHREAMRSAIAGILEISLDRVSVKATTTEGLGFTGRREGIAAMATATVVAEQPSVRTGMGFDVHRLAKGEELWLCGVLVPHDKGLAGHSDADVAIHALVDALLGALAAGDIGDHFPPSDPQWKGASSDRFLAHAASLVRTAGFDIGNVDVTIICEAPKIGPHKLAMRTALARILGIDTGAVSVKATTTERLGTTGRGEGIAAQAVATIVRTGTGFDVHAFAEGDYVILCGVKVPHDRTLKGHSDADVGLHAVTDAIYGALADGDIGRHFPPSDPKWKGAASHVFLTHAAGRAAERGYRLSNVDCTLVCERPKIGPHAAAMAGEIARLVKVAPERVSVKATTSETLGFTGREEGIAALATVTLVPES",
    "response#3": "MNRVGQGYDVHRLAEGEELWLCGIKLDHHKGLAGHSDADVALHAITDAVLGAIGAGDIGTHFPPSDPEWAGARSAQFLEHAVKLAANAGYTIGNVDLTLICEAPKIGPHRPAMRSQVAIITGLSEDAVSIKATTTERLGFTGREEGIAAQAIARMAAEALFKEEQGMSGHVRTGMGFDVHRLAKGEELWLCGVLVPHDKGLAGHSDADVAIHALVDALLGALAAGDIGDHFPPSDPQWKGASSDRFLAHAASLVRTAGFDIGNVDVTIICEAPKIGPHKLAMRTALARILGIDTGAVSVKATTTERLGTTGRGEGIAAQAVATIVSRV"
  },
  {
    "reference": "MMSSVSTESKLQQAVSLKGVDPETCMIVFKNHWAQVVKILEKHDPLKNTQAKYGSIPPDEASAVQNYVEHMLFLLIEEQAKDAAMGPILEFVVCENIMEKLFLWSLRREFTDETKLEQLKMYEMLVTQSYQPLLHHKPILKPLMMLLSSCSGTATPAVEGKLVVLLNQLCSILAKDPSILELFFHTSEDQGAANFLIFSLLIPFIHREGTVGQQARDALLFIMSLSAENSMVANHIVENTYFCPVLATGLSGLYSSLPTKLEEKGEDWHCILKDDWLLLPALVQFMNSLEFCNAVIQVAHPLIRTQLVSYIYNGFLVPVLAPALHKVTVEEVMTTTAYLDLFLRSISEPALLEIFLRFILLHRHENVHILDTLTSRINTPFRLCVVSLALFRTLIGLHCEDVMLQLVLRYLVPCNHMMLSQRWAVKERDCYSVSAAKLLALTPVCCASGITLTLGNQERDYILWSKCMHDGSGSEEQLLPETPCSPSSPSPPPPPAPAACIVEYGKALDISYLQYLWEAHTNILHCMRDCRVWSALYDGDSPDPETFLQSLSEESRENSGHPEARLPQQSVRTSGQTKDKSQSELEWDDSYDTGISSGADVGSPGPDDEVETPAPPAPIDPPKHIQEMKKNAILLFKGSYIEESDFQDDVMVYRLCAEKDTEDTTEPQKDTSEPQGDTLEPLEDTSEQQEDTSEQLEDTSELQEDTAEPQGDTADPTAEAQPKLQPEAQSLPTSNGPLSSPDPETESQPSRESSDLCQNTFSEAKPEN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell.; Probable component of the FTS/Hook/FHIP complex (FHF complex).2. The protein should be able to modulate protein localization to perinuclear region of cytoplasm in a way that leads to a desirable outcome.3. The protein design should emphasize Polar residues, Acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "EKREIGLLNDRLADYIEKVRFLKAQNHVLSHDIEILRRGFSGGGHISSFFESEISNCTVISERILSSRTKFQADITGLEGEIEIYRKKWLEALKVVRSHREDHHVNLDKLAEVEAQIALFNRKIRIVEEDVLRIRRETGGIQNDIGRIHSQIHAEIALKNERHHSVQMLLQRVQVIQTENSSRIEQELIYIHRDTTLENRDYFRQELQAAMRDIRANYEAMSIRSREDIEIAYRKRIDEIRVIPAHVHIEDYREELLSIRTTLSTTHSRLAEIESRNAWLLTTITDLKHQQAEEARIFEATLDAKQATYEQFKERCTELSIQMEKLCDHETSLQAELERYRVLLNGANVTS",
    "response#2": "TGPFISVVLSKLENMLENSLHVNLLLIGIITQLASYPQPLLRSFLLNTNMVFQPSVRSLYQVLASVKNKIEQFASVERDFPGLLIQAQQYLT",
    "response#3": "EKREIGLLNDRLADYIEKVRFLKAQNHVLSHDIEILRRGFSGGGHISSFFESEISNCTVISERILSSRTKFQADITGLEGEIEIYRKKWLEALKVVRSHREDHHVNLDKLAEVEAQIALFNRKIRIVEEDVLRIRRETGGIQNDIGRIHSQIHAEIALKNERHHSVQMLLQRVQVIQTENSSRIEQELIYIHRDTTLENRDYFRQELQAAMRDIRANYEAMSIRSREDIEIAYRKRIDEIRVIPAHVHIEDYREELLSIRTTLSTTHSRLAEIESRNAWLLTTITDLKHQQAEEARIFEATLDAKQATYEQFKERCTELSIQMEKLCDHETSLQAELERYRVLLNGANVTL"
  },
  {
    "reference": "MGHSPSRHNACGGGGGDGESPPSPLPSRFERFRRRLRLRHRDRAGRPGGDAHASESGTGRAIAVDEFAGIARIRIVKEKKVVVETNGPHIARISVFETNRFSKNTLVGYCEVDLFELLTKDLDEHSEVLSLLDPSSSATIVGSISISCYIEDPVETEQSFARRVLAIVDYNEDGELSLSEFSDLMKAFGNKLAVAKIEELFRQADKNGDGIVDMDELAALLANQQEKEPLISNCPVCGEILGKHDKINDMIHMTLCFDEGTGNQIMTGGFLTDKQASYGWMFKLSEWAHFSSYDVGLHSGSTASHILVFDRRTKRLVEEVIDGKIVLSMRALYQSKVGLTLIDTGVKDLLKNLSEKQGKKMSSPESAKDIPKFLELFKDQINLDEVKDPLESFKTFNEFFVRQLKPGARPIACYEQDTIATCAADSRLMTFSSVDESTRLWIKGRKFSIEGLLGKDVHSDALCNGSLVIFRLAPQDYHRFHVPVSGTLEKFVEIPGCLYTVNPIAVNSKYCNVFTENKRVVSIISTSEFGKVAFVAIGATMVGSIEFLKEEGDYVHKGDEFGYFAFGGSTVICVFEKDAIEFDADLLANSARSLETLVSVGMRLGVSTRNRDLQPQELEKCSLE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a binding site that is specific to the target Ca(2+) ligand.2. Ideal protein should have a binding affinity for key enzymes in the phosphatidylethanolamine from CDP-diacylglycerol: step 2/2, phosphatidylethanolamine biosynthesis, Phospholipid metabolism pathway to enhance the overall efficiency of the pathway.3. The Charge relay system; for autoendoproteolytic cleavage activity active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.4. The designed protein should contain one or more C2, EF-hand domains that are essential for its function.5. The designed protein should have calcium ion binding, phosphatidylserine decarboxylase activity within endoplasmic reticulum membrane, vacuolar membrane to regulate phosphatidylethanolamine biosynthetic process, protein autoprocessing.6. The protein must exhibit the following characteristics: Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer); Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.. The designed protein sequence is ",
    "response#1": "MLKVVVLKARNLAAKDKSGTSDPYLVVTLGDSKNATQSVPKTLNPEWNTTIQMPVNSASALLLDCVCWDKDRFGKDYLGEFDLALEDIFTQDRTEIFLKINANRAADLVSTSSTTNSLLSPHKYSSRKSINPVLIVQVNQTITSTSKKLNTNQPSWDDAILVPLKHNDFSQMMILTIWDKHKRYKNYLGELRLSIRDLFYEDGKFKLRVEINEGRNLAAKDKSGTSDPFVIVRLGSQKKRTEVIRRDLNPRWDAQFDFSVDASSFPLSVQLICYDKDRITKEYIGETTISTGMLVSGEKPKFWIPLRD",
    "response#2": "MKSKSGNSSPPGESNLGLALKVVIMKARDLAAKDRSGTSDPYLVVTLGDARIVTHSVAKTLDPEWNVIQELPVNSINSLVLDVICWDKDRFGKDYMGEFDLALEEIFQNDRPEQEPRWYDLKPNVKEGKVKSGTSDPYLVLTEFVLRVTVFKGKDLAAKDKSGTSDPYLVLTLGDAKEATSTINKTLNPEWNQTFELPIVGVQSLVLEAVCWDKDRFGKDYMGELDVAVEELFTTGQVATEPHWYPKKTDFNFSAANDILGIVMLEIQSAADLPRLKNMTRTGWDMDPFVVISFGKKVFRTRVIRHSLNPTWDEKLLFHVRRYETAFKIQLTVLDWDKLSSNDHVGDASFNVSELLANAPQKDEILKVVVVKARNLAAKDKNGTSDPYLVVTLGDSKNATQSVSKNLNPEWNVTLQMPVNSANALLLDCVCWDKDRFGKDYLGEFDLALEDIFTADEVDILVENLKVVVVKARNLAAKDKNGTSDPYLVVTLGDSKNATQSVSKNLNPEWNVTLQMPVNSANALLLDCVCWDKDRFGKDYLGEFDLALEDIFTADEVDIGPQWFPLDTDESGRISKVEIKADTDESGCISKVEIKSVIFLEILHITDLPPLRNMTRTGFDVDPFVIISFGRSTFRTQSKRHTLNPTYNERVIFPVLKHEQSFSIGFTVMDKDKISTHDFVADGQFEIKPLLELAPRPDVLFMEIQKITDLPPEKNLTRTSFDMDPFVVTSLGRKTYRTRVVRHCLNPVYDEKLVFQVMRHEIKYQMSFTVVDRDKFSGNDYVGAVNFPLESVV",
    "response#3": "FLKINANRAADLVSTSSTTNSLLSPHKYSSRKSINPVLIVQVNQTITSTSKKLNTNQPSWDDAILVPLKHNDFSQMMILTIWDKHKRYKNYLGELRLSIRDLFYEDGKFKPSSEISPALTPANPDTRPLMLKASVISGRNLAPKDRNGLSDPYLVVSLGEARQSTPTISKTLNPEWKVSFELPIVGVPLLECICWDKDRFGKDYMGEFDIPLEDIFSAGQIQPKPQWYELKDSVLRVEINEGRNLAAKDKSGTSDPFVIVRLGSQKKRTEVIRRDLNPRWDAQFDFSVDASSFPLSVQLICYDKDRITKEYIGETTISTGMLVSGEKPKFYNFNELSGKGRSGTSDPYLVLTLVLRVTVFKGKDLAAKDKSGTSDPYLVLTLGDAKEATSTINKTLNPEWNQTFELPIVGVQSLVLEAVCWDKDRFGKDYMGELDVAVEELFTTGQVATEPHWYPVDTDESGCISKVEVLKVVIMKARDLAAKDRSGTSDPYLVVTLGDARIVTHSVAKTLDPEWNVIQELPVNSINSLVLDVICWDKDRFGKDYMGEFDLALEEIFQNDRPEQEPRWYPLDTDESGRISRIEV"
  },
  {
    "reference": "MNMLMILSVNIILSTCLIMIAFWLPQLNVYTEKANPYECGFDPMSSARLPFSMKFFLVAITFLLFDLEIALLLPLPWAIQMHNINMMMSTAFILVSILALGLAYEWLQKGLEWTE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. The designed protein sequence is ",
    "response#1": "SMNLFMFYLFFESSLIPTLFLILGWGYQPERLQAGVYLLFYTLLVSLPLLVGIFYLYKNCNTMNFYLLSNYMFNCNLLYLAMILAFLVKMPMFLVHLWLPKAHVEAPVSGSMILAGIMLKLGGYGLLAVFSFLQLIGLKYNYIWVSISLIGGVLISLVCLRQTDLKALIAYSSVAHMGIVLGGLMTLSYWGLSGSYTLMIGHGLCSSGLFCLANITYERLGSRSLLINKGLLNFMPSMTLWWFLLSVCNMAAPPSLNLLGEISLLNSIVSWSWLTMISLSFLSFFT",
    "response#2": "SMNLFMFYLFFESSLIPTLFLILGWGYQPERLQAGVYLLFYTLLVSLPLLVGIFYLYKNCNTMNFYLLSNYMFNCNLLYLAMILAFLVKMPMFLVHLWLPKAHVEAPVSGSMILAGIMLKLGGYGLLAVFSFLQLIGLKYNYIWVSISLIGGVLISLVCLRQTDLKALIAYSSVAHMGIVLGGLMTLSYWGLSGSYTLMIGHGLCSSGLFCLANITYERLGSRSLLINKGLLNFMPSMTLWWFLLSVCNMAAPPSLNLLGEISLLNSIVSWSWLTMISLSFLSFFS",
    "response#3": "SMNLFMFYLFFESSLIPTLFLILGWGYQPERLQAGVYLLFYTLLVSLPLLVGIFYLYKNCNTMNFYLLSNYMFNCNLLYLAMILAFLVKMPMFLVHLWLPKAHVEAPVSGSMILAGIMLKLGGYGLLAVFSFLQLIGLKYNYIWVSISLIGGVLISLVCLRQTDLKALIAYSSVAHMGIVLGGLMTLSYWGLSGSYTLMIGHGLCSSGLFCLANITYERLGSRSLLINKGLLNFMPSMTLWWFLLSVCNMAAPPSLNLLGEISLLNSIVSWSWLTMISLSFLSFFAV"
  },
  {
    "reference": "MSSGKVLQVIGPVVDVEFPLDEKLPEINDALKIKESDGKTLTTEVALELGDGVVRTIAMDGTDGLQRGMEVENTGASISVPVGDDTLGRVFNVLGEPVDNGPKFGPDAKRMPIHRDAPKYDDLNNATEILETGIKVIDLLAPYVRGGKIGLFGGAGVGKTVLIQELIHNIAQGHNGISVFTGVGERTREGNDMYYEMKASGVLEKTAMVYGQMNEPPGARMRVALTGLTIAEYFRDVKGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATEMGQLQERITSTKKGSITSIQAVYVPADDYTDPAPATTFAHLDATTNLERRLTQIGIYPAVDPLASTSTALTPEIVGKEHYEVATQVQHVLQRYHELQDIISILGMDELSDEEKTIVARARRIQNFLSQSFSVASQFTGLPGKYVPLKETIKGFKEILAGKYDDLPEEAFRLVGPIEDVVEKAKKMKAETDEDSSED",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein can be targeted to plasma membrane, proton-transporting ATP synthase complex, catalytic core F(1) and perform ATP binding, proton-transporting ATP synthase activity, rotational mechanism, proton-transporting ATPase activity, rotational mechanism.2. The ATP binding site should be located in a region of the protein that is accessible to the ligand.3. We need a protein that can perform ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate with high efficiency.4. The protein must exhibit the following characteristics: Produces ATP from ADP in the presence of a proton gradient across the membrane; The catalytic sites are hosted primarily by the beta subunits.. The designed protein sequence is ",
    "response#1": "RGGKIGLFGGAGVGKTVLIMELINNIAKAHGGVSVFGGVGERTREGNDLYMEMKESKVINEQNIAESKVALVYGQMNEPPGARMRVGSTALTMAEYFRDVNKQDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLGTEMGTLQERITSTKEGSITSIQAVYVPADDLTDPAPATTFAHLDATTVLSRGGSAAKVPIGIKAVDAMIPIGRGQRELIIGDRQTGKTSIAIDTIINQRSNYEAGNPVYCIYVAIGQKGSTVAALVNTLQEKGAMDYTIVVSATASDPAAMQYFAPFAGAAIGEYFRDSGRHALVVYDDLSKQAVAYREVSLILRRPSGREAYPGDIFYLHSRLLERAAKIINQPEVAREMNDLPESMRDKVKGGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLETDLFNQGNRPAINVGISVKGFKGLVEGKYDHLPEQAFYLVGDINEAIQLKAMKKVAGTLKIDQAQFRELESFSKFGGEMDAVTAFTIDKGQKNTQLLIQPQYSPMPVEEQISILYCGTQGLLKEVPLDKVHEFEARFLQELRTSHQRDVLDVLKTGVINDEIRGILEKTAKL",
    "response#2": "KGGKIGFFGGAGVGKTVLVMELIRNIAIEHHGFSMFAGVGERTREGNELYLDMQEAEVLDNTVLVFGQMNEPPGARFRVALSALTMAEYFRDVEGRDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLATDMGELQERITSTKKGSITSVQAIYVPADDITDPAPATTFTHLDATVVLSRRGVGLVAVDAMIPIGRGQRELIIGDRQTGKTAIATDTILNQKGKDVICVYVAIGQKASSIAQVVNTLTERGALDYTIIVAATADSPATLQYLAPYTGAALAEYFMYTGRHTLVIYDDLSKQAQAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDQLGSGSMTALPIVETQEGDVSAYIPTNVISITDGQIFLSADIFNSGLRPAVNVGISVKAAVKEFFGSSQLSQHPRIKEEAGRMKLDYLQFLELEIFTRFGARLESGMEARIRRGRLLREILRQERLQPLPPEFQLAWMTAYNEGLLDAMPPGSVQHALNELNSALRNGLATGQYGS",
    "response#3": "MKKVAGTLKIDQAQFRELESFSKFGGEMDAVTAFTIDKGQKNTQLLIQPQYSPMPVEEQISILYCGTQGLLKEVPLDKVHEFEARFLQELRTSHQRDVLDVLKTGVINDEIRGILEKTAKEA"
  },
  {
    "reference": "MIYPVILCAGVGKRMKSVFPKVMHCVLDKPMLWWVLRSFRGLIDNEPIVVVGKNKDLISQYFAGEKINYVVQSEPLGTAHALLCAYKSLKSFSEEDYFLVMPGDMPLIKQETIKNLCKMSEQNNDITFITCKVDDPKGYGRILRDKDNNFLRIVEEKDAKSDELNIKEVNVGIYLIKIALLKLLYNIENNNAQGEYYLTDLLELALKMGYKIGTMEIFDQKEVLGVNSQKDLLEAQNEAKRTIIDVHLDNGVQIFDINSTWIGPEVCISSGAKIMPASVIYGKSKIGSSTIGPFSNVENSTIGDNCNVIYSVIRNSTIGNSVNIGPFSHIREETVVHDNIRIGNFVELKKTEIRNNSKVSHLSYLGDTSVGSNVNVGAGTITCNYDGFDKHRTTIEDDVFVGSDSILVAPVKLSKGSMVAAGSVITRDVPEDSLGIGRASQVNKAGWVKIFRSTKAKKKDD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit magnesium ion binding.2. The protein design should take into account the physiological conditions of the UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway.3. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "GVTIIDPSSTYIETDVVIGRDSVIYPGTLIGAGTVIGEECIIGPHSQLKNCQVGDRVEIKHSVLMDSRVDSDATVGPFAYIRPHSHIGEGAKVGDFVEIKNTSLGKGSKVSHLTYLGDAEIGENVNVGCGTVTVNYDGVNKYKTIVKDRSFIGCNVNLVAPVTVGEGAYIAAGSTVTTDVPDGALAIARERIG",
    "response#2": "AVVLAAGQGTRMKSKLYKVLHPVCGKPMVQHVVDSLKELDMARIVVVVGHGADKVKEQLGNAVEYAMQEEQLGTAHAVMQVAPILKGKKGTTLVLSGDEPLISSETLSALVAHHEAKQAAGTILTRIMDNPTGYGRIIRGEDGAVERIVEQKDANEEEQRVREINTGTYCFDNEKLFSALEKVDNNNTQGEYYLTDVIEILKKQGERVNTAAITYEETLGVNSRVQLAEAEEIMRRRINERHMRAGVRIIDPDVVYIDPQVKIGKDTIIYPFTFIEGDTTIGEDCIIGPMTRIKNSKIGNNVIINRSEVKKAEVKDNVSVGPYARLREGTTLDTNVKIGNFVETKKTVMGQNSKAQHLTYLGDATIGKNVNIGAGTITCNYDGKKKHPTFIEDNVFIGSNSSLVAPVKIGKNSITAAGSTITNNVPENSLAIARERGVTINRDTVLEEALEQGATLVDPESVWFSHDTQLGRDSTIEPHVVFGPGVVIEDFATIRAFCHIEGATIASGCEVGPFARLRPGTVLEQGAKVGNFVEVKKARLGEGAKANHLSYIGDADVGAKANIGAGTITCNYDGFGKHRTEIGAGAFIGSNTALVAPVSVGEGAIVGAGSVITRDVAADELAVARGQGVPARIIRPGNA",
    "response#3": "MPGVTIIDPNNTYIDCNVVIHNDSIIYPGNILQGKTVIKENCVLYPNSRIVDSIIEKSVVIQNSVILQSNIGENTTVGPFAYIRPDSNIGSAVRIGDFVEIKKSTIGNNTKVSHLTYIGDAEVGERCNFGCGTVVVNYDGKEKHKTIVGDDVFIGCNANLVSPVEVKDNSYIAAGSTITDEVPRGALAIARSKG"
  },
  {
    "reference": "MPELPEVEVVRRGLQSHAVGAAIEAVEVLHPRAIRRHILGSEDLIGQLTGQTIASAERRGKYLWLVLEPAGVGLVVHLGMSGQMLVQPPTVDWEKHLRIRLALDSGADLRFVDQRTFGGWSISPLVEVDGTMLPESVAHIARDPMDAAFDLESVVKVLRGKHTEIKRAILDQTVVSGIGNIYADESLWRAKIHGNRIAESLTRPKLRELLTAAHSVMGEALDQGGTSFDALYVNVNGESGYFDRSLSAYGQENLPCPRCGAPIKREKFMNRSSFSCPRCQPTPRARRIA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Proton donor; for delta-elimination activity active site of the designed protein must be optimized for substrate binding and specificity.2. The designed protein must be able to perform class I DNA-(apurinic or apyrimidinic site) endonuclease activity, zinc ion binding, damaged DNA binding under specific conditions.3. The protein must exhibit the following characteristics: Acts as DNA glycosylase that recognizes and removes damaged bases. The designed protein sequence is ",
    "response#1": "PELPEVETVRRGLIPAMEGVRIARVTAHRNDLRFPLQTDFVARLGGRVVTGLGRRAKYLLADLDSGDVLLMHLGMSGSFRVAMDGGQEATPGIFHHPRSESRTHDHVVFEMDNGAVISFNDPRRFIG",
    "response#2": "PELPEVEIVKQGLNQLTLNKRILGGEVLLERTLAYPISVADFLRGLEGKAIAQWHRQGKYLLAQLYKWGKKNSKLQEYENEDGWLGVHLRMTGQLLWVNPEESLHKHTRVRLFFGHNSSGDKDSSNYELRFVDQRTFGKML",
    "response#3": "PELPEVETVRRGLIPAMEGVRIARVTAHRNDLRFPLQTDFVARLGGRVVTGLGRRAKYLLADLDSGDVLLMHLGMSGSFRVAMDGGQEATPGIFHHPRSESRTHDHVVFEMDNGAVISFNDPRRFL"
  },
  {
    "reference": "MPTEQTHNVVHEANGVKLRETPKEFFERQPNKGHIHDVNQYKQMYEQSIKDPQGFFGPLAKELLSWDHDFHTVKSGTLKNGDAAWFLGGELNASYNCVDRHAFANPDKPALICEADDEKDSHILTYGDLLREVSKVAGVLQSWGIKKGDTVAVYLPMNAQAIIAMLAIARLGAAHSVIFAGFSAGSIKDRVNDASCKALITCDEGKRGGRTTNIKKLCDEALVDCPTVEKVLVYKRTNNPEIHLTEGRDYYWDVETAKFPGYLPPVSVNSEDPLFLLYTSGSTGTPKGVVHSTAGYLLGAALSTKYIFDIHPEDILFTAGDVGWITGHTYALYGPLLLGVPTIIFEGTPAYPDYGRFWQIVEKHKATHFYVAPTALRLLRKAGEQEIVKYDLSSLRTLGSVGEPISPDIWEWYNEFVGKNQCHISDTYWQTESGSHLIAPLAGVVPNKPGSASYPFFGIDAALIDPVTGVEIEGNDAEGVLAIKDHWPSMARTVYKNHTKYMDTYMNPYPGYYFTGDGAARDHDGYYWIRGRVDDVVNVSGHRLSTAEIEAALIEDKKVSEAAVVGIHDDITGQAVIAYVALKEGNSDEDSEGLRKELVLQVRKTIGPFAAPKSVIIVQDLPKTRSGKIMRRILRKVSSNEADQLGDISTLSNPQSVEGIISAFGAQFGKK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have acetate-CoA ligase activity to facilitate acetyl-CoA biosynthetic process from acetate. The designed protein sequence is ",
    "response#1": "KGGVSIISQSGAVLNAILDIAPLLNIGFSKVVSIGNKADIQESDLLEYFLDDEDTKIVVLYIEGLKDKRFLKVAKKLSKKKPIIALKSGRTEVGKKAAKSHTGSLAGEDVIYEAAFKEAGIIRAYTFEELVDLIHLR",
    "response#2": "VIDLLKQRVQSAGKKPVIIFPEGWSPTVMEAVNQLQQAGILTPPVIFRTRSEVPAGFNTAIKHYVIEEMDLTKYANFVYEKRKHKGMEMREAQKFVRDASSLAATLVALNEVDGEVCGKEYATKDTLRPALQLLGTGNFVSSVFIMEKNEERLYFTDCAFAVYPSPQELAVVAENTFKFAQSMGEPELKMVFLSYSTLGSGKGEAVDKVVSATQIFLEKHPELKANVCGELQFDSAFVEKVRKQKAPNLTWNGSANIYVFPNLDAGNIGYKIAQRLGGYEAIGPIVLGLARPFNDLSRGASVSDVFNVGIITGH",
    "response#3": "VIDLLKQRVQSAGKKPVIIFPEGWSPTVMEAVNQLQQAGILTPPVIFRTRSEVPAGFNTAIKHYVIEEMDLTKYANFVYEKRKHKGMEMREAQKFVRDASSLAATLVALNEVDGEVCGKEYATKDTLRPALQLLGTGNFVSSVFIMEKNEERLYFTDCAFAVYPSPQELAVVAENTFKFAQSMGEPELKMVFLSYSTLGSGKGEAVDKVVSATQIFLEKHPELKANVCGELQFDSAFVEKVRKQKAPNLTWNGSANIYVFPNLDAGNIGYKIAQRLGGYEAIGPIVLGLARPFNDLSRGASVSDVFNVGIITQ"
  },
  {
    "reference": "MRLCFASNNAHKLDEIRPLLPAHVELLSLADIGCAEELPETQDTLEGNALQKARYVWDNYGVSCFADDTGLEVTALGGAPGVYSARYAGPQRSSADNLQKLLRELDGSAERTARFRTVIALVSGAADQWTFAGEVPGHITTAPRGEGGFGYDPVFEPENRGRTFAEMTLDEKNALSHRARAVAQLAAFLREQYSVE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a low Km value for dITP + H2O = dIMP + diphosphate + H(+).2. The substrate binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein must be able to perform dITP diphosphatase activity, XTP diphosphatase activity, nucleotide binding, metal ion binding under specific conditions.4. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. The designed protein sequence is ",
    "response#1": "MKFVLATGNAHKAKELGAILADLGIEVLPQSAFDVPEPVEDGLSFVENALIKARHAARLTGLPALADDSGLAVDALGGAPGIYSARYAGEPKSDARNNAKLLEALREVPEAQRTARFHCVLVLVRHADDPEPLVCEGRWSGRILHEPRGTGGFGYDPLFFVPERGCSAAELDPALKNALSHRGRALRQLLGRL",
    "response#2": "MSILSGKVALITGGGTGIGRATSLAFAREGAKVIVNYLNSEEAAEETVRIIREEGGDAIAVQADVSKRENVDRLFEETIKAFGRVDIVCSNAGIEVLGTIAELTDEHVDRMFAVNIRGPVLGIQAAARHMKEQKSGRIILMS",
    "response#3": "MKKIILATHNQGKYREFKDILKDL"
  },
  {
    "reference": "MGQGSGDDGVPPAPFSSAAAAAHSPPHSPLSVGVSSASSATSSSSTPPSSTSPAGVSASGARNVETDWKQSGDENLAELCIFHVPDKSVSLPNPKRAECTLPMNLILKSSSKNRKKSSIWSSDHIPRGVRFGPLVGEIRLVDVDTALVCPAEASMAGGGPAQEDVPFDEAPEEWKIYSPSGGRLNKTICVKDDARSNWMKYVAAAEEEDFQNLVAAQIGNDIYFYTVKKIEANTELSFWFSRDYARKLNYSTRPYVRVRRPATQLIPSAPPASASTAIASLAETIVAIDYSVKKLIESPIDTLSTDASSASDEEMIDVEEQESCTRPVAEVTRPNVIQNPVVRPVATKVNNFPGIPVRLGNFYASPLVDFKEFMRKSLQLKLVDTSMFVSPVAQTTAAITATGGRSGQPIDVQPVLAATAGAHFGNYAAIYGSQDFQHELSKPLYTSASPAFGGGGGMGGGFGMGGSAHTSSFHQLPFVNHSSSSHNDSSFNGVPNYVQQQENGKTRYACKDCNKTFGQLSNLKVHVRTHTGERPFKCEICTKEFTQLAHLQKHHLVHTGERPHRCDICDKRFSSTSNLKTHLRLHNGQKPYTCDVCDAKFTQYVHLRLHKRLHANERPYSCGTCGKKYISPSGLRTHWKTTTCKEEDMKDSMRDDLMDIKGEIDEGSMSGSGYGNLGIFENTLNSELKRPLMPIETIYSKYNLPNASLLGQGPSGMQEQQAPPPTSQQQQHMMYGNTMGHMGQGSHLQGPPPPPQHFQMDHSGMQNG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must have at least one SET domain.2. For general function, the protein need meet that Transcription factor which binds to enhancer elements in the promoter region of genes; Regulates the timing of dorsalward migration of the distal tip cells of the hermaphrodite gonad by inhibiting precocious unc-5 and lin-29 expression which in turn prevents early dorsalward turning; Plays a role in male tail tip morphogenesis.; Regulates the expression of the transcription factor bed-3 to control vulval development; Promotes terminal differentiation in the hypodermis and is involved in regulation of gonadal outgrowth and entry into the dauer stage3. A protein that contributes to negative regulation of transcription by RNA polymerase II, regulation of gene expression, negative regulation of distal tip cell migration, positive regulation of apoptotic process involved in development, regulation of transcription by RNA polymerase II by functioning in cytoplasm, nucleus with RNA polymerase II cis-regulatory region sequence-specific DNA binding, DNA-binding transcription repressor activity, RNA polymerase II-specific, metal ion binding.4. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "VKRPMNAFMVWSRGQRKKMALENPKMHNSEISKRLGTEWKMLSEQEKRPFIDEAKRLRAIHMKEHPDYFPATREIISEIYQKQFGLLHIFLKNTFDEYAECDVQEQKRICAMFYPVLWEIESCYWTYRNMPVQPEYETLMMCTQTTYIDSKNVRYWLGNTTGLNESDIQAVEARLENLLTKARNLVLEPMHKLIIKEFEFITLLALNIWAPRNHRGCVSEDRAEQVRDALFDDLHYLYCDGLKIDKYSSRMGEMMCLHTEVQNADS",
    "response#2": "LENRELWKEFSSVGTEMIITKAGRRMFPACRVSVTGLDPEARYLFLLDVIPVDGARYRWQGRRWEPSGKAEPRLPDRVYIHPDSPATGAHWMRQPVSFHRVKLTNSTLDPHGHLILHSMHKYQPRIHLVRAAQLCSQHWGGMASFRFPETTFISVTAYQNPQITQLKIAANPFAKGFRGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGGGSAGGPPRGGPRPGPGGPGPGGPRPSPRGGPRPGGGGPGARGGPRPGPRNPFAKGFRGGPGGRGGPGPFPGQFGGPFFPGGPGGFPGGFPGGFPGQGGFPGQFPQGGFPGQFPQGGFPGQFPGQFPQGGFPQQFPGNFPGGFPGGFPQGGFPQQFPGQFPQGGFPQQFPGNFPGGFPQGGFPQQFPGQFPQGGFPQQFPGNFPGGFPQGGFPQQFPGQFPQGGFPQQFPGQFPQGGFPQQFPGQFPQGGFPQQFPGQFPQGGFPQQFPGQFPQGGFPQQFPGQFPQGGFPQQFPGQFPQGGFPQQFPGQFPQGGFPQQFPGQFPQGGFPQQFPGQFPQGGFPQQFPGQFPQGGFPQQFPGQFPQGGFPQQFPGQFPQGGFPQQFPGQFPQGGFPQQFPGQFPQGGFPQQFPGQFPQGGFPQQFPGQFPQGGMP",
    "response#3": "LENRELWKEFSSVGTEMIITKAGRRMFPACRVSVTGLDPEARYLFLLDVIPVDGARYRWQGRRWEPSGKAEPRLPDRVYIHPDSPATGAHWMRQPVSFHRVKLTNSTLDPHGHLILHSMHKYQPRIHLVRAAQLCSQHWGGMASFRFPETTFISVTAYQNPQITQLKIAANPFAKGFRPATREIISEIYQKQFGLLHIFLKNTFDEYAECDVQEQKRICAMFYPVLWEIESCYWTYRNMPVQPEYETLMMCTQTTYIDSKNVRYWLGNTTGLNESDIQAVEARLENLLTKARNLVLEPMHKLIIKEFEFITLLALNIWAPRNHRGCVSEDRAEQVRDALFDDLHYLYCDGLKIDKYSSRMGEMMCLHTEVQNADEKQSRNLKPSLENRELWKEFSSVGTEMIITKAGRRMFPACRVSVTGLDPEARYLFLLDVIPVDGARYRWQGRRWEPSGKAEPRLPDRVYIHPDSPATGAHWMRQPVSFHRVKLTNSTLDPHGHLILHSMHKYQPRIHLVRAAQLCSQHWGGMASFRFPETTFISVTAYQNPQITQLKIAANPFAKGFRKRAMPATREIISEIYQKQFGLLHIFLKNTFDEYAECDVQEQKRICAMFYPVLWEIESCYWTYRNMPVQPEYETLMMCTQTTYIDSKNVRYWLGNTTGLNESDIQAVEARLENLLTKARNLVLEPMHKLIIKEFEFITLLALNIWAPRNHRGCVSEDRAEQVRDALFDDLHYLYCDGLKIDKYSSRMGEMMCLHTEVQNAD"
  },
  {
    "reference": "MLKGKTVVLGVSSSIAAYKIANLASMLVKQHANVRVIMTQNATNFITPTTFETLTGQKCLVDTFDRNFEFQVEHVSLAKQADIFMIAPATANVIAKVSHGLADDMLTTTFLACKAPKYLAPAMNTQMYENPITQDNLNICRKYGMHVIEPASGYLACGDTGAGKMPEPETLFEYILQELACEKDLAGKKVLVTAGPTREAIDPVRYITNHSTGKMGYAIARAAARRGAEVTLVSGPVDLKAPMGVKLVPVVSAKDMFDAVTSVSLEQDAIIKAAAVADYRPTVVGAEKTKKSDGNMNIELERTDDILAWLGAHRREGQVLCGFSMETQNMLENSRAKLVKKNVDMIVANSLRTAGAGFGTDTNIVTVITKDGAEELALMSKDQVAHELLNRIFAGKH",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit metal ion binding.2. Ideal protein should have a binding affinity for key enzymes in the coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway to enhance the overall efficiency of the pathway.3. The presence of DNA/pantothenate metabolism flavoprotein C-terminal domains is critical for the interaction of the designed protein with its intended targets.4. The designed protein should have a high specificity for CTP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "HILLLVSGSIAAYKSLDLVRAFVKMGAKVKVVMSKQAVRFVTPLSFEALSHYPVLTHKSERWDLSDPNCPNHISYAKWGEVVLVAPASANTLAKLAHGLADQLLSATFLASHAPKILAPSMNTQMLEACATQENLERLRSWGCKIIEPREDLLACNTQGKGAMADILELVCRELARLHAVPLAGRRALVTSGPTREPIDPVRFIANHSSGKQGHAIAEALSRLGAETILVSGPVTLPDPPGLSAVHVESARDMLRACEQALPVDVAVCAAAVADWRVKDAKDQKIKKKNGKSPSLTLVENPDILKTLSTAAGNRRPSLVVGFAAETENIVKNATEKRRRKGCDWLLANDVSPASGTFGGDSNRLHLWAGKKVVVSGGGSLEKIDSVRYISNFSSGLQASCLALALYFWGAEVSLIASAFALDLPAGIGRLSVQSGADFLQALQERANLQDPPFLFMLAAISDYKPTTPHKGKLKKQDLGTSWSLECVQNVDILASLEGFIKIGFKAEEGGTNAITNARKLLESPQNGGKGCLAVCLNTLDSQPFGSAQNQMWLIDAAGVEQLPEMDKHAAAQRLLDLVAVRLAGAK",
    "response#2": "LGVGGGIAAYKACEVLRGLTESGHDVRVVPTEAALNFVGAATFEALSGHPVHTGVFTEVPEVQHVRVGKEADLVVVVPATADLLARAAHGLAGDLLTNTLLTARCPVAFFPAMHTEMWEHAATRDNVALLRSRGMVVAEPASGRLTGADTGKGRLADPAEIVAACRALL",
    "response#3": "NVLLAVTGSIAAYKAIEILRGLRNEQKSVKVILTKGAEQFINKLSFESLGAHEVLTDEDQFSVDKDGISIHLSLSRWADCIIIVPASADIIAKIRAGIADSLLLTVVLASKKPLFIAPCMNENMLLNKITADNIEHLKNNRVMFIEADEGQLADFKIGKGRLQEPNKIVSLITE"
  },
  {
    "reference": "MSPTNVQVTDYHLNQSKTDTTNLWSTDDDASVMEAFIGGGSDHSSLFPPLPPPPLPQVNEDNLQQRLQALIEGANENWTYAVFWQSSHGFAGEDNNNNNTVLLGWGDGYYKGEEEKSRKKKSNPASAAEQEHRKRVIRELNSLISGGVGGGDEAGDEEVTDTEWFFLVSMTQSFVKGTGLPGQAFSNSDTIWLSGSNALAGSSCERARQGQIYGLQTMVCVATENGVVELGSSEIIHQSSDLVDKVDTFFNFNNGGGEFGSWAFNLNPDQGENDPGLWISEPNGVDSGLVAAPVMNNGGNDSTSNSDSQPISKLCNGSSVENPNPKVLKSCEMVNFKNGIENGQEEDSSNKKRSPVSNNEEGMLSFTSVLPCDSNHSDLEASVAKEAESNRVVVEPEKKPRKRGRKPANGREEPLNHVEAERQRREKLNQRFYSLRAVVPNVSKMDKASLLGDAISYISELKSKLQKAESDKEELQKQIDVMNKEAGNAKSSVKDRKCLNQESSVLIEMEVDVKIIGWDAMIRIQCSKRNHPGAKFMEALKELDLEVNHASLSVVNDLMIQQATVKMGNQFFTQDQLKVALTEKVGECP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.2. The protein must exhibit the following characteristics: Binds to the G-box (5'-CACGTG-3') of promoters.3. The protein design should aim to mimic or enhance the function of natural proteins involved in positive regulation of DNA-templated transcription, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "QRRHVILIGEPGTGKSMLAQSMVDFLPKSELEDILVFPNPEDPNKPKIKTVPAGKGKEIVRQYQIKAEREKRDRSRSIMFVIFSVVLLGIIAAIVLRSITLIFFAIMAAAFLYMAMAFNPVIRNEKAMVPKLLVSHSSTDKPPFVDSTGAHSGALLGDVRHDPFQSGGLETPAHERVEAGNIHKAHKGVLFIDEINLLRPEDQQAILTALQEKKYPISGQSERSAGAMVQTEPVPCDFVLVAAGNYDAIRNMHPALRSRIRGYGYR",
    "response#2": "VNGLAVVGADTGMAEYTGVVLPIVAEVTPAEHKGAGNIIATGKLGDIAKEAVLNVSAVFKKITGKDISNMDIHIQFVGTYEGVEGDSASVSIATAVISAIENIPVDQSVAMTGSLSVRGDVLPVGGVTAKVEAAIEAGMAKVIVPELNYNDIILDAEHINRIQIIPARTIEDVLKVALG",
    "response#3": "GLTQDWPVAIVGIGNLGAALANYGGFASRGFRVAALIDADPAMAGTPVAGIAVQHTDDLDRIISDNGVSIGVITTPPGAAQQVCDRLVAAGVTSILNFAPTS"
  },
  {
    "reference": "MVSVCRLLLVAALLLCLQAQLSFSQHWSHGWYPGGKREIDSYSSPEISGEIKLCEAGECSYLRPLRTNILKSILIDTLARKFQKRK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein localizes to the extracellular region.2. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "LFGVDTDSCERWMNCKSEFLKKYMHKVANDLPSCPCSYPTEVAYSTAEIYDRIKRKDFRWKDASGPKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCFDDSMQLITRGKGAGTPNLISVEFSAELHYKVDILPWIMCKGDWSRYNEVRPPNNGQKCTENPSEC",
    "response#2": "LFGVDTDSCERWMNCKSEFLKKYMHKVANDLPSCPCSYPTEVAYSTAEIYDRIKRKDFRWKDASGPKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCFDDSMQLITRGKGAGTPNLISVEFSAELHYKVDILPWIMCKGDWSRYNEVRPPNNGQKCTENPSEC",
    "response#3": "LFGVDTDSCERWMNCKSEFLKKYMHKVANDLPSCPCSYPTEVAYSTAEIYDRIKRKDFRWKDASGPKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCFDDSMQLITRGKGAGTPNLISVEFSAELHYKVDILPWIMCKGDWSRYNEVRPPNNGQKCTENPSEC"
  },
  {
    "reference": "MLPRLICINDYEQHAKSVLPKSIYDYYRSGANDEETLADNIAAFSRWKLYPRMLRNVAETDLSTSVLGQRVSMPICVGATAMQRMAHVDGELATVRACQSLGTGMMLSSWATSSIEEVAEAGPEALRWLQLYIYKDREVTKKLVRQAEKMGYKAIFVTVDTPYLGNRLDDVRNRFKLPPQLRMKNFETSTLSFSPEENFGDDSGLAAYVAKAIDPSISWEDIKWLRRLTSLPIVAKGILRGDDAREAVKHGLNGILVSNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVLKALALGAKAVFVGRPIVWGLAFQGEKGVQDVLEILKEEFRLAMALSGCQNVKVIDKTLVRKNPLAVSKI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have a binding site that is specific to the target FMN, glyoxylate ligand.2. The protein design should prioritize the formation of helix-helix-helix-strand-strand-strand-strand-helix-helix-strand-helix-strand-strand-helix-strand elements.3. The protein design should incorporate a binding pocket that specifically recognizes FMN to enhance enzymatic efficiency.4. For general function, the protein need meet that Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate; Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2; The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine; this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones; Can also catalyze the oxidation of glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, albeit with much lower catalytic efficiency5. The designed protein should contain at least one Microbody targeting signal motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "MELITNVSEYEQLAKQKLPKMIYDYYASGAEDQWTLKENREAFSRILFRPRILIDVSRINMATNVLGFNISMPIMIAPSAMQKMAHPEGELATARAASAAGTIMTLSSWSTSSVEEVNSAAPGIRFFQLYVYKDRNIVRQLVRRAELAGFKAIALTVDTPRLGRREADIKNRFNLPPHLVLKNFEALDLGKMDKTNDSGLASYVASQVDRSLSWTDVKWLQTITSLPILVKGVMTAEDTRLAVESGAAGIIVSNHGARQLDYVPATISCLEEVVREAKGRLPVFLDGGVRRGTDVFKALALGASGVFIGRPVLFSLAVDGEAGVRKVLQMLRDELELTMALSGCTSLAEITRNHVITDSSDGSYFVVDLYKLLEEHDRNKTASFTFGALDPIHVTQMAKYLDTVYISGWQSSSTASTSNEPSPDLADYPYDTVPNKCEHLWFAQLFHDRKQHEERFRMTPEERAKTPYTDFLRPIIADADTGHGGITAIIKLTKLFIERGAAGIHIEDQAPGTKKCGHMAGKVLVPIQEHINRLVAIRTAADIFGSDLLCVARTDSEAATLLTSTIDHRDHYFIIGSTNKDLQPLSEVMFQAEQRGLLKVMLAAVEEDWTKKAGLKLFHEAVIDEINSGYYPNKQALIAEFTTKVHPLSNTSNKEARALAKKLTGKDIFFDWEAPRVREGYYRYQGGTQCAVNRARAYAPYADIIWMESKLPDYAQAKEFADGVKAQWPEQWLSYNLSPSFNWNKAMSVLYQETYIQKLAKLGYVWQFITLAGLHTTALAVDNFAR",
    "response#2": "LYKLLEEHDRNKTASFTFGALDPIHVTQMAKYLDTVYISGWQSSSTASTSNEPSPDLADYPYDTVPNKCEHLWFAQLFHDRKQHEERFRMTPEERAKTPYTDFLRPIIADADTGHGGITAIIKLTKLFIERGAAGIHIEDQAPGTKKCGHMAGKVLVPIQEHINRLVAIRTAADIFGSDLLCVARTDSEAATLLTSTIDHRDHYFIIGSTNKDLQPLSEVMFQAEQRGLLKVMLAAVEEDWTKKAGLKLFHEAVIDEINSGYYPNKQALIAEFTTKVHPLSNTSNKEARALAKKLTGKDIFFDWEAPRVREGYYRYQGGTQCAVNRARAYAPYADIIWMESKLPDYAQAKEFADGVKAQWPEQWLSYNLSPSFNWNKAMSVLYQETYIQKLAKLGYVWQFITLAGLHTTALAVDNFAREYTLNNLKFAVTVDKDNSDLQIRYQDVIKARKNEEATVPSLLGEEKKTNPFLRWDQPALQMITGMNDPARVFGKIRGMKDNYPLEYTLNNLKFAVTVDKDNSDLQIRYQDVIKARKNEEATVPSLLGEEKKTNPFLRWDQPALQMITGMNDPARVFGKIRGMKDNYPLP",
    "response#3": "MELITNVSEYEQLAKQKLPKMIYDYYASGAEDQWTLKENREAFSRILFRPRILIDVSRINMATNVLGFNISMPIMIAPSAMQKMAHPEGELATARAASAAGTIMTLSSWSTSSVEEVNSAAPGIRFFQLYVYKDRNIVRQLVRRAELAGFKAIALTVDTPRLGRREADIKNRFNLPPHLVLKNFEALDLGKMDKTNDSGLASYVASQVDRSLSWTDVKWLQTITSLPILVKGVMTAEDTRLAVESGAAGIIVSNHGARQLDYVPATISCLEEVVREAKGRLPVFLDGGVRRGTDVFKALALGASGVFIGRPVLFSLAVDGEAGVRKVLQMLRDELELTMALSGCTSLAEITRNHVITDSKLFVRPRGWHLPEAHILIDGEPAIGCLVDFGLYFFHNHAAFGAGQGAGFGPLCDLPKMEHSREARIWNGVFQRAEKAAGIEPGSIRATVLVETLPAVFQMNEILHELREHSAGLNCGRWDYIFSYVKTFRAHPDRLLPDRALVGMAQHFMRSYSHLLIHTCHRRGVHAMGGMAAQIPIKDDAAANEAALELVRKDKLREVRAGHDGTWAAHPGLIPAIREVFEGHLGGRPNQIGDAAGHEGASVKEEDLILHFHGTGLYKLLEEHDRNKTASFTFGALDPIHVTQMAKYLDTVYISGWQSSSTASTSNEPSPDLADYPYDTVPNKCEHLWFAQLFHDRKQHEERFRMTPEERAKTPYTDFLRPIIADADTGHGGITAIIKLTKLFIERGAAGIHIEDQAPGTKKCGHMAGKVLVPIQEHINRLVAIRTAADIFGSDLLCVARTDSEAATLLTSTIDHRDHYFIIGSTNKDLQPLSEVMFQAEQRGLLKVMLAAVEEDWTKKAGLKLFHEAVIDEINSGYYPNKQALIAEFTTKVHPLSNTSNKEARALAKKLTGKDIFFDWEAPRVREGYYRYQGGTQCAVNRARAYAPYADIIWMESKLPDYAQAKEFADGVKAQWPEQWLSYNLSPSFNWNKAMSVLYQETYIQKLAKLGYVWQFITLAGLHTTALAVDNFAR"
  },
  {
    "reference": "MDFLPIFLHIKQRPCLVVGGGEVAARKVALLLQAEASVTVVAPALGTALTKLHQAGHIRHAARTFSADDLAHQYLVIAATNDTGLNHRVALLANARQIPINVVDRPELCTFVVPSIIDRSPVIAAVSTGGASPVLARLIRARLETLIPAAFGRLAILAAEFRGKVKQRFSHPPDRRIFWEKVLQGPVAELMFAGKDAAARNALEQALNAETGAPHEMGEVYLVGGGPGDPDLLTFRALRLMQQADVVVHDRLVSPAVLDLTRRDAERIYVGKERDNHALPQEEINRFLVRLAKQGKRVLRLKGGDPFIFGRGGEEIDTLAEEGVPFQVVPGITAASGCAAYAGIPLTHRDFAQSCVFVTGHLQDGKVNLNWDTLIRPDQTIVFYMGLRGLALICRGLIDRGLADTTPAALIQQGTTPRQRVFTGALRTLPDIVARVDHIKPPTLLIVGGVVSLHDKLNWFSREE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have sirohydrochlorin ferrochelatase activity, precorrin-2 dehydrogenase activity to facilitate cobalamin biosynthetic process.2. The protein should be able to bind NAD(+) ligand in a variety of conditions3. The protein design should consider the endogenous proteins and enzymes present in the sirohydrochlorin from precorrin-2: step 1/1 pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "VGAGPGDPELLTLRAMRLMQQADVVVFDRLVSPEILDMVRRDATRIYAGKERSKHVMPQESINQLLVRLAKEGKRVLRLKGGDPFIFGRGGEEIETLSSHGIPFQVVPGITAATGAASYAGIPLTHRDFAQSCVFVTGHLKDGTVNLDWPMLARPNQTIVVYMGLLGLSELCRQLIAHGLPSTTPAAIVQQGTTHKQRVLTGTLQTLPNLTAAARLTPPTLIIVGEVAG",
    "response#2": "GEVYLVGGGPGDPDLLTFRALRCMQQADVVVYDRLVAKPILDMTRRDAERIYVGKERDNHAMRQEEINKLLARLAKEGKRVVRLKGGDPFIFGRGGEEIDTLAEEGIPFQVIPGITAASGCASYSGIPLTHRDYAQSVTFVTGHLKDNSMNLNWQQLAQPHQTVVFYMGLKGLPVITRELQSHGRSADTPAALIQQGTTHMQRVLTGTLETIESIVEREQPKPPTLIIVGEVVELAS",
    "response#3": "VGAGPGDPDLLTIKALNELQKAEVVLYDQLVSPEIMALIRPEAELIAVGKKAGHHSVPQEQINQLLVDQALTGKRVVRLKGGDPFMFGRGAEELETLKEAGIAYQVVPGITAAAGATAYAGIPLTHRDHAQSAVFITGHSKQDGKEPNWASLAQSRQTLVIYMGLMRSAHIQQRLIENGLASSTPVALIERGTTPQQRVVRGTLSELGVLAEQVVSPSLMVIGEVL"
  },
  {
    "reference": "IMYDCCSGSCSGYTGRC",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that localizes to extracellular region, host cell postsynaptic membrane and can perform toxin activity with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "EVNWG",
    "response#2": "GCCHPACGKHFSC",
    "response#3": "SCCHPACGKHFSG"
  },
  {
    "reference": "MQTYGNSAVTYGWWAGNSGVTNRSGKFIAAHAAHTGLIAFWAGAFTLFELARFDPSVPMGHQPLIALPHLATLGIGFDETGTFVGGSAVVAVAVCHLVGSMAYGAGGLMHSLLFSSDMQESSVPQARKFKLEWDNPDNQTFILGHHLIFFGVACIWFVEWARIHGVYDPSIGAIRQVEYDLNLSHIWDHQFDFLTIDSLEDVMGGHAFLAFLEITGGAFHIATKQVGEYTKFKGAGLLSAEAILSWSLAGIGWMAVVAAFWSATNTTVYPVEWFGEPLALKFGISPYWVDTVDLGSAHTSRAWLANVHYYFGFFFIQGHLWHALRAMGFDFKRVTSALSNLDTASVSLK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that The antenna complex functions as a light receptor, it captures and delivers excitation energy to photosystems II and I; The Prochlorales pcb genes are not related to higher plant LHCs.2. The designed protein should have chlorophyll binding within photosystem I, plasma membrane-derived thylakoid membrane to regulate photosynthetic electron transport chain. The designed protein sequence is ",
    "response#1": "EGGDSG",
    "response#2": "MPIGVPKVPFRSPGESDAE",
    "response#3": "MPKIVKL"
  },
  {
    "reference": "MPYETPQAAAVAWLPYSQLATTLDQPTLDWLFDEGSLTRRLTRLSHDHFSVTPLFEGWQPLRDDECAALGLAPGEEGWVREVYLRGHDQPWVFARSVAGRSVLERGGLDLETLGTRSLGELLFCDQAFIRHPLEACRYPQTWLPTKVAHEGLWGRRSRFERSGLDLLVAEVFLPALWQAAKEETR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECR",
    "response#2": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECR",
    "response#3": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECN"
  },
  {
    "reference": "MRPAQDNQGSFLGRISIRRNQFVDVNNEQEQEDLELFQKHIADRFTELLSPPQPPPSDEINTVASVAATEQIMSVTWLRKLMDVFLCCEAEFKAILLMGRDPTQISKPPFDRLVPEMLDRSIKALDICTAVVNGIDSVRHYQRLAEIAVTALEQRPLGDGNVRRAKRALANLVVALSLEDKENVSGGGGGGGGGNKTTERSWSFGRRSGGSSAASKGGATIGQLKSSSWAVGRNWSAAKQIHAMTANLTPPRGNEAAGLPQPMFIMSTVMVFVMWVLTAAVPCQERSGLANHLPVPPKHLNWAQSLIGIHEKIGDEWKKKEKKGSAGLMEEMTRMEKLGHSLMEFADGFHYPAEKDAAESAAVQVAEMAEICRRMEEELVPLQQQIREVFHRIVRSRAEILEVLEQAGKVSAPVV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be designed to localize specifically to membrane in order to perform its role in seed coat development, mucilage biosynthetic process involved in seed coat development more effectively.2. The protein must exhibit the following characteristics: Required for seed coat mucilage deposition..3. The protein design should emphasize Polar residues compositional bias.4. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "TCCVSLSAFYNETIVPCPTCSCGCQNSSQAGTCVDPKIASVVPALGKNNLEPLLQCTQHMCPIRVHWHVKTSYKEYWRVKVAITNFNYNMNYSQWNLVVQHPNFDNLTKLFSFNYKPLNPYLNINDTAMLWGIKFYNDFLSQAGPVGNVQSELLFQKNPLEFTFEKGWAFPRRIYFNGDNCVMPPPDSYR",
    "response#2": "TCCVSLSAFYNETIVPCPTCSCGCQNSSQAGTCVDPKIASVVPALGKNNLEPLLQCTQHMCPIRVHWHVKTSYKEYWRVKVAITNFNYNMNYSQWNLVVQHPNFDNLTKLFSFNYKPLNPYLNINDTAMLWGIKFYNDFLSQAGPVGNVQSELLFQKNPLEFTFEKGWAFPRRIYFNGDNCVMPPPDSYR",
    "response#3": "TCCVSLSAFYNETIVPCPTCSCGCQNSSQAGTCVDPKIASVVPALGKNNLEPLLQCTQHMCPIRVHWHVKTSYKEYWRVKVAITNFNYNMNYSQWNLVVQHPNFDNLTKLFSFNYKPLNPYLNINDTAMLWGIKFYNDFLSQAGPVGNVQSELLFQKNPLEFTFEKGWAFPRRIYFNGDNCVMPPPDSYR"
  },
  {
    "reference": "MLKGKKIVIGVTGGIAAYKACDITSSLKKLGAEINVIMTESATHFVNPQTFQALSQNPVTVDMFDTPRYWDIEHISLAQKADLILVAPATANIIGKVINGIADDMLSTTIMASTAKVVFAPAMNTKMYLNPIVQNNIEGLKALGYAFIDPASGRLACGELGVGKLCEVEKIIVYVTELLSMEADLKGKKLLITAGPTIEAIDPVRYLTNHSTGKMGYAIAEAAHGRGAEVTLISGPTNLKPPAGMEFIRVNTAEEMYDRVLENFEENDIIIKSAAVADYRPEATHPQKMKKQDDDLYIKLVRNPDILMELGKRKGNKYLVGFAAETNDIIEYAKNKLKKKNLDLIVANDLTEEGAGFGKDTNVVYLIDSKEDTTKLDKMTKLEVAHKVLDKIVKSI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit phosphopantothenoylcysteine decarboxylase activity, metal ion binding.2. The protein must bind to Mg(2+) in order to perform its enzymatic function.3. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A4. The designed protein should contain one or more Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains that are essential for its function.5. The protein should be optimized for activity in Cofactor biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "LAGQRVLVTLGPTREPWDGVRFWSNPSSGIMGACMAMAAHLRGADVTVVAGPVDLVFPSGITVIPTQTALEMHAACLDLWPLMDIGCATAAVADFRPEPHGPGKFKKSGATSLHIDFVPNPDILKSLGDSKRADQRLIGFAAETCDPRDEAARKLKAKGLDLIAANDVSRHGSGFRTPTNEMYV",
    "response#2": "MRIVLGVAGGIAAYKACHVVRELRERGHTVRVVPTRAALEFVGAATWEALSGQPVATDVFTRVDEVEHVRIGQDAELVVIAPATADLLARLRAGIADDLLTATVLTTTAPVVAAPAMHTEMWQNPATVDNVSTLRSRGMHVIDPASGRLTGADSGPGRLPEPGDIVAAAVAAALDPAAARARLDGRHVVVSAGGTREALDPVRFLGNRSSGLQGYALARTAAARGARVTLVAANVALPDPAGVRVVVVESALQLREAVLSAADRADAVVMAAAVADFRPAGYRPSKIKKRDGASAPSIELVQNPDVLAELGARPHAGQVIVGFAAETDDVLANGRAKLARKGCDLLVVNQVGADLAFGRPDNAAVLLDADGREVPVPRGSKEELADTVWDLVAPRLG",
    "response#3": "MRIVLGVAGGIAAYKACHVVRELRERGHTVRVVPTRAALEFVGAATWEALSGQPVATDVFTRVDEVEHVRIGQDAELVVIAPATADLLARLRAGIADDLLTATVLTTTAPVVAAPAMHTEMWQNPATVDNVSTLRSRGMHVIDPASGRLTGADSGPGRLPEPGDIVAAALAAFGAALDAR"
  },
  {
    "reference": "MSTPGTLHIVATPIGNLGDLSPRAQHILRTVTMICAEDTRHTRQLLAHFGIERPLLALHAHNEDTLAERIITRLISGESLALVSDAGTPLISDPGFLLVRAARAAGIRVTPVPGPSALIAALSVSGLPSNRFTFEGFLPAKPTARRDRLTHLAHEPRTLIFYEASHRIAECLTDLATIFGSMRIAVVARELTKIFETVLDGTLATLQTQVANDENQRKGEFVIIVQGAADQDAAKITEGRRIYALLKEHLPPSSAAKLAAEITGAPRKALYGG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA. The designed protein sequence is ",
    "response#1": "MLYIVGTPIGNLEDITYRAVDVLKSVNVIFAEDTRVTIKLLSRYKINKKMISCNAVTENKKIRLLLDYLAKGDSVAFVSDAGTPGLSDPGSLLVAAAFREGYKVCPIPGVSSFNTIVSVNPFRDKSVLFEGFLPNKGLKRFKKIAELYKRGDAFVLLESGHRLLKLLVEISSVSLDAKILIGREMTKIL",
    "response#2": "MLYIVGTPIGNLEDITYRAVDVLKSVNVIFAEDTRVTIKLLSRYKINKKMISCNAVTENKKIRLLLDYLAKGDSVAFVSDAGTPGLSDPGSLLVAAAFREGYKVCPIPGVSSFNTIVSVNPFRDKSVLFEGFLPNKGLKRFKKIAELYKRGDAFVLLESGHRLLKLLVEISSVSLDAKILIGREMTKIF",
    "response#3": "LYIVATPIGNLGDITLRAIETLRRCDAVACEDTRMTGKLLKHLGISKSLWRYDDHSDAKAREKIVAAISEKAVALVSDAGTPLISDPGYRLVRDARDASLPVTTIPGACAAIAGLTLSGLPSDRFLFAGFLPVKDKARREMLEKLAPVDASLIFYETGPRLLKSLAAIDEMLPNREISVARELTKLHEECRRR"
  },
  {
    "reference": "MSRKYNVAVVGANGAVGEEILAILEELNFPLNKLVPLASQRSVGKSVDFKGRSVPIQELTNTIFQEENIEIALFSAGGSVSAQFAHAAVEAGAVVIDNTSHFRMDKNTPLVVPEVNPEDIAQWTNTGIIANPNCSTIQMVQTLKPLNDVYGLQRVDVSTYQATSGAGKTAMEELVTQMQSFFAFKLDECEHKSFAHQIALNVIPQIDVFEENGYTKEEMKMVNETTKIMHKNIELSATCVRVPTLRGHAEALTLTFDKDVNAKEAREILSKAPNIIILDKPQESLYPMPSLCMGLNETFVGRIRVDNYRKNILHLFVVADNLRVGAATNAVRIAQKWVEMQGEIND",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The Acyl-thioester intermediate, Proton acceptor of the protein must be engineered to enable effective catalysis of L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.2. The designed protein should have a binding site that is specific to the target phosphate, substrate ligand.3. A protein with NADP binding, aspartate-semialdehyde dehydrogenase activity, NAD binding is needed to enhance 'de novo' L-methionine biosynthetic process, isoleucine biosynthetic process, threonine biosynthetic process, lysine biosynthetic process via diaminopimelate in a specific context.4. The designed protein should contain one or more Semialdehyde dehydrogenase NAD-binding domains that are essential for its function.5. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.6. A protein that is capable of catalyzing reactions within the L-threonine biosynthesis, L-threonine from L-aspartate: step 2/5 pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MARVALLGATGMVGQRFVKLLENHEMFELSVLAASPSSAGKKYQDAAHWCIEGEVPEKFREMTVVKADLENVKKEDNVDFVFSALPSDVARIVEAEFAKAGIPVISDASAYRMEEDVPILIPEVNPDHLELLAPIDRQYKLHQVLVSTYQAASGAGQRAMNELFDEAQQRHEGEEETAAILPVKGAEHHYPLAFNLLPQIDVFEDDGFTHEEWKMIHETKKILRGDMNDTELKVSATCVRVPVPIGHGESVYFETHQTPAVPDMQDLLAHSKGVVLQDDPAHQVYPQPINAAGHGETFVGRMRADRENPGAYHMWVVADNLLDRGIVPSVNCTCVRVPVMRSHLAPIDRQYKLHQVLVSTYQAASGAGQRAMNELFDEAQQRHEGEEETAAILPVKGAEHHYPLAFNLLPQIDVFEDDGFTHEEWKMIHETKKILRGDMNDTELKVSATCVRVPVPIGHGESVYFETHQTPAVPDMQDLLAHSKGVVLQDDPAHQVYPQPINAAGHGETFVGRMRADRENPGAYHMWVVADNLLSRGDADAEGLAPIKAKYGLTRVIVSTYQAASGAGQSAWEELVAETTAHNAGQEMHANILPVKNAEHHYPLAYNLLPQIDVFEDDGYTHEEWKMIHETKKIMFGDKNSKAVKVTGTAVRVPVPIGHGEAVYFETEAGTGATSGDIQSILSKAPGVVLEDNPKEQLYPQPINAEGKRETFVGRVRPDLENDDSFHMWVVSDNLLDL",
    "response#2": "MTPLNIGVVGATGQVGAVVRRLLEERDFPVASIRYFASARSAGTTLPWKGEDIVVEDAAVADPTGLDVAIFSAGATLSRAQSPRFAAAGVTVIDNSSAWRMDPEVPLVVSEVNPEAIAQAALPSGIVANPNCTTIQLVRALNHLHKALKIKRIVVSTYQSVTGTGKKAVDQLMNERAGIEGEKAYAYPIDLNVIPHIDVFEENGYTKEEMKMVNETKKILGDDSIKVTATAVRIPVMGGHSESVNVEFEKDFTLEEVYRLLQETPGVVVVDDTANLKYPMPMDAHGKDEVLVGRIRRDETQPNTINIWVVADNLRKGAALNAVQIAELL",
    "response#3": "MVVAILGATGVVGTQMLQCLEERDFPVSRLVPLASARSAGKTLTFRGEEVAIQEARPEAFEGVDIVLGAAGDDIAKALLPEAAARGAVCVDNSHAFRLDPDVPLVVPEINAGDIANAQLAKGIKAFVGGNCTVSLMLMALNPLHQKYGIERVFISTYQSVTGTGKKAVDQLFAESKGKYCDRIYPYPIYQNAIPQCDSFEENGYTKEELKLIREPKKIMGVKNMGVSATAVRVPVIGGHSESVQISFKKDFELDELREILKNTPGLILQDNPKNNYYPMPIHSYGKDEVFVGRLRRDLSQENSLLLWIVADNLRWGAAEPLRRMLRILLEA"
  },
  {
    "reference": "MAARVCACLWMMLLIAQAEATLENLVVLNAASVAGAHGLLSFLVFFCAAWYIKGRLVPGAAYALYGVWPLLLLLLALPPRAYAMDREMAASCGGAVFVGLVLLTLSPYYKVFLARLIWWLQYFITRAEAHLQVWVPPLNVRGGRDAIILLTCAVHPELIFDITKLLLAILGPLMVLQAGMTRVPYFVRAQGLIRACMLVRKVAGGHYVQMAFMKLAALTGTYVYDHLTPLRDWAHAGLRDLAVAVEPVVFSDMETKIITWGADTAACGDIISGLPVSARRGKEILLGPADSFGEQGWRLLAPITAYSQQTRGLLGCIITSLTGRDKNQVDGEVQVLSTATQSFLATCVNGVCWTVYHGAGSKTLAGPKGPITQMYTNVDQDLVGWPAPPGARSMTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPVSYLKGSSGGPLLCPSGHVVGIFRAAVCTRGVAKAVDFIPVESMETTMRSPVFTDNSSPPAVPQTFQVAHLHAPTGSGKSTKVPAAYAAQGYKVRVLNPSVAATLGFGAYMSKAHGIEPNIRTGVRTITTGGPITYSTYGKFLADGGCSGGAYDIIICDECHSTDWTTILGIGTVLDQAETAGARLVVLATATPPGSITVPHPNIEEVALSNTGEIPFYGKAIPIEAIKGGRHLIFCHSKKKCDELAAKLTGLGLNAVAYYRGLDVSVIPTSGDVVVVATDALMTGFTGDFDSVIDCNTCVTQTVDFSLDPTFTIETTTVPQDAVSRSQRRGRTGRG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should emphasize Polar residues compositional bias.2. The designed protein must have at least one RdRp catalytic, Peptidase C18 domain.3. The designed protein should be involved in modulation by virus of host G1/S transition checkpoint, protein complex oligomerization, suppression by virus of host JAK-STAT cascade via inhibition of STAT1 activity by localizing to host cell plasma membrane, host cell perinuclear region of cytoplasm and exhibiting RNA helicase activity. The designed protein sequence is ",
    "response#1": "EVKNISTGYMVTNDCTNDSITWQLQAAVLHVPGCVPCEKVGNASQCWIPVSPNVAVQRPGALTQGLRTHIDMVVMSATLCSALYVGDLCGGVMLAAQMFIVSPQHHWFVQDCNCSIYPGTITGHRMAWDMMMNWSPTATMILAYAMRVPEVIIDIISGAHWGVMFGLAYFSMQGAWAKVVVILLLAAGVL",
    "response#2": "WRNDSGLYILTNDCANRSIVYEADDVILHTPGCIPCVQTGNTSQCWTPVTPTVAVRYVGATTASIRSHVDLLVGAATMCSALYVGDVCGAVFLVGQAFTLRPRRHHTVQTCNCSLYPGHLTGHRMAWDMMMNWSPAIGMVVAHVLRIPQTLFDIIAGAHWGILAGLAYYSMQGNWAKVAIIMIMFSGVT",
    "response#3": "EVKNISTGYMVTNDCTNDSITWQLQAAVLHVPGCVPCEKVGNASQCWIPVSPNVAVQRPGALTQGLRTHIDMVVMSATLCSALYVGDLCGGVMLAAQMFIVSPQHHWFVQDCNCSIYPGTITGHRMAWDMMMNWSPTATMILAYAMRVPEVIIDIISGAHWGVMFGLAYFSMQGAWAKVVVILLLAAGVLFSNYFFN"
  },
  {
    "reference": "MLSPVNVSIESLNLMTLVPMLIPIIGALFIIVIDLFKSEQDKSLYVMLSLLILGVDFVALVDSAGVFANNGTIMGVFDMMLIDGLAILSQFIIVGASMLFIPLALTHKRFHEFSYPEFFALFLFMIAGFQFMVSTDNLILIFVGLETASLALYTLIAMHNRDKSFEAAVKYFTMGALAAGFFSFGSMVFYALTGSVEINQIATVLTANNYADIGFVLVGVVFLLAAFGFKLSMVPFHTWTPDVYEGSSAALAGYMSIVPKIAAFIVAMRLFEFLIHSGVVWLEVILYMGVVVTMTMANIWALVQSDVKRMLAYSSISHAGFVMAAILIGTTQSNSALFLYWILFSFTNLGSFSMLWISRQKNLPAHQQSDHSYDKFAGMVKTSPVAASIMALFMLSLAGIPPFALFWGKMYLMSSAITGGYTVLALIMALNSAIAGYYYLKLIVYMFMKDPVVENNGHVYSANATLPLKTIIGIAAIGTIFAFVAVNQLIEFVTLFVYNSGY",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization.2. The ideal protein would be one that plays a critical role in ATP synthesis coupled electron transport through NADH dehydrogenase (ubiquinone) activity, quinone binding within plasma membrane.3. The protein must exhibit the following characteristics: The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone; Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.; NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain.4. We need a protein that can perform a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) +   NAD(+) with high efficiency. The designed protein sequence is ",
    "response#1": "DIGAMTVFLYAFREREQILQFFEKICGARLTVSYPRIGGVRVDIKEHVLDEIYKFMDLMLIRVDEYETLLTENRIWIARTRGVGVIAPEDAVLLGLTGPALRGSGVYYDIRKQIPYDAYSEIDFEVPLGEKGDTYDRYLCRIREMRQSVLIVKQCIEKMPEGKILSDKSPDIDLPHQAKRKIEPGDSLWNGFIAFSEEKQEIMPKGEIYSAIEAPKGELGFYIVSDGSGRPYRMRVRAPSFIHISAIPKLCEGHLLADVIAIIGTLDIVMGEADFEGTYKAALK",
    "response#2": "DVGALTPPLWGFEEREKLMVFYERASGARMHANYFRPGGVHIDCPQQLIDDIGAWCDPFLKVCDDLNDLFIENRIFKQRNVDIGVISLEDCWRWGFSGVMVRGSGAAWDLRKAQPYECYEEMDFDIPVGRHGDNYDRQVIRMEEMRQSTKIMKQCVEKLSQASGKGPVATPQHKVVPPSRAEMKRSMEALIHHFKLYTEGFHVPAGEVYCGVEAPKGEFGVYLVSDGTDKPYRCKIRAPGFAHLSAMDFLCRKSMLADVSAILGSLDIVFGEVDPRWV",
    "response#3": "DIGAMTVFLYAFREREQILQFFEKICGARLTVSYPRIGGVRVDIKEHVLDEIYKFMDLMLIRVDEYETLLTENRIWIARTRGVGVIAPEDAVLLGLTGPALRGSGVYYDIRKQIPYDAYSEIDFEVPLGEKGDTYDRYLCRIREMRQSVLIVKQCIEKMPEGKILSDKSPDIDLPHQAKRKIEPGDSLWNGFIAFSEEKQEIMPKGEIYSAIEAPKGELGFYIVSDGSGRPYRMRVRAPSFIHISAIPKLCEGHLLADVIAIIGTLDIVMGEADE"
  },
  {
    "reference": "MGNLPEFSIRDLVEAGVHLGHKAGRWNPAMAPYIYGVHKYKDIHVIDLRKTLVLLRDALSVLYDVVLKRGRVLFVGTKVQASNIVAEEATRCGQYYVNHRWLGGMLTNWETVSSSIRRLVEFERLINNNEGQFTKKELLMLDKQRGKLERSLGGIREMGGLPHALFVIDTNKEHIAIREANKLKIPVVAILDTNSDPGGVDYPIPGNDDSVRSIDFFCRAVSRTILEAIRSDLASSGVNIAAKGSDVAESYAGTAADAGHTPVSETLSAGGPTAGASPYIPEVGPSVVEVDTHKSAALPDSRPNDADVPAEDGVLPDPGVADGAALE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have structural constituent of ribosome within small ribosomal subunit to regulate translation. The designed protein sequence is ",
    "response#1": "MPFAPM",
    "response#2": "MKLIGLIINTHGLKGELKVNPQTDNIDRFKKLKRVYIAGEEKQVEGCKFLNDKVVLKIQGIDGIEIANKYRNKYIEVKREI",
    "response#3": "MGRMHAPGKGLSSSALPYRRSVPTWLKLSSEDVKEQIYKLAKKGLRPSQIGVILRDSHL"
  },
  {
    "reference": "MKYFDIMSLECKKIGIEFTEEKYGKFMRYKELLQEWNKVMNLTSIVDDEEIVKKHFIDSIKIFECEHIVKAKRIIDVGTGAGFPGLPIAIMNDDIEVVLLDSLQKRVNFLNEVIKELNLKNISTVHGRAEDFGVDKDYREKFDVAVSRAVANMAVLSEFCLPFARVGGYLVALKGPGINDEISTAKKAIKVLGGTLDKVINVEFENEDFQHNLVVIKKQNGTPKKYPRKAGKVSKNPII",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Specifically methylates the N7 position of a guanine in 16S rRNA..2. The protein should be able to bind S-adenosyl-L-methionine ligand in a variety of conditions3. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining rRNA (guanine-N7-)-methyltransferase activity. The designed protein sequence is ",
    "response#1": "MLRSSAR",
    "response#2": "MPK",
    "response#3": "MNSPLLDTIVNL"
  },
  {
    "reference": "MKDRKKDIRALTKQQLQEFFVSQGDKSFRGTQVYEWLWSKAAHSFEDMTNLSKSTRQMLDENFVINHIRVDRMQRSSDGTIKNAVKLHDSLTVESVLIPTKTRTTACVSSQVGCSLDCQFCATAKLKRMRNLNPDEIYDQVVAIDNESRLYFDRPLSNIVFMGMGEPLMNYNNVMKSIEKITSPEGLGMSPKRITISTSGVPKMIKKLADDEAKIKLAVSLHSARNEVRTQIMPFNETFPLEDLRESLEYWYSKTGSRITYEYIVWKDINDTREDAQALVSFCKYVPCKVNLIEYNPIDDGNFQQAAGEATRMYEQMLERNNITVTVRRSRGKDIDAACGQLANKSA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The design of the protein must focus on the precise arrangement of amino acids in the S-methylcysteine intermediate to enable effective catalysis of adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.2. A protein with tRNA (adenine-C2-)-methyltransferase activity, tRNA binding, rRNA (adenine-C2-)-methyltransferase activity, rRNA binding is needed to enhance rRNA base methylation in a specific context.3. The designed protein should be able to compete with endogenous proteins or inhibitors for S-adenosyl-L-methionine, [4Fe-4S] cluster binding.4. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.5. The protein design should incorporate a binding pocket that specifically recognizes [4Fe-4S] cluster to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "MHTPESLNAWCRDVGMPPFRATQILEWVYAKGVADPDAMSNLGKADRDLLRNQITFLSGAVVRHQRATDGTQKLLVEWDDFGAKAADAERRVSLMPGSDTSRQTECVMIPSLDADERHAPARFTACISSQVGCPVGCRFCASGIGGLDANLSAGRIVEQVWRLSRLEGVRRISNVVFMGMGEPLANLGSVVPAIRTLAAPWGLGISARKITVSTVGLPKAIDKLAEQLDLPVTLALSLHAPTDEIRRELIPWAEYTTIRELLDACRRWFERTGREVTLEYTLLRGVNDQPAHARELARLARTLRANVNLIRYNEVSGVEFERPRDADVRTFQDVLREGGINAHIRASRGRDIAAACGQLRHEQALAA",
    "response#2": "MNIYGMTRYDLEQYFINNNMHKFKALQVFEWLYIHKVKSFFEMTNIKKELQEQLERDFSTRMIHIEKKQQDQETSKYLFKLFDNNYVEAVLMKHDYGLSVCVSSEVGCNMGCAFCESGRLKKVRELNSYEMVEQILLIEQDIGKRVSSIVIMGIGEPLDNYDNVMKFIRIINDPKGLAIGARHITLSTCGLVPKIKELSNLDLQINLAISLHAPNNELRSKIMPINKAYNLNTLIDTIKEYIEKTNRRVTIEYVMLDNINDSEKEAKELSSLIKNLNVYVNLIPYNETNHLEFKRTSKNKIMKFYDYLKKQGINVTIRREFGSNIDAACGQLRSKEVLTW",
    "response#3": "MNIYGMTRYDLEQYFINNNMHKFKALQVFEWLYIHKVKSFFEMTNIKKELQEQLERDFSTRMIHIEKKQQDQETSKYLFKLFDNNYVEAVLMKHDYGLSVCVSSEVGCNMGCAFCESGRLKKVRELNSYEMVEQILLIEQDIGKRVSSIVIMGIGEPLDNYDNVMKFIRIINDPKGLAIGARHITLSTCGLVPKIKELSNLDLQINLAISLHAPNNELRSKIMPINKAYNLNTLIDTIKEYIEKTNRRVTIEYVMLDNINDSEKEAKELSSLIKNLNVYVNLIPYNETNHLEFKRTSKNKIMKFYDYLKKQGINVTIRREFGSNIDAACGQLRSKEVIS"
  },
  {
    "reference": "MFEDLKPHLQELRKRLMVSVGTILVAFLGCFHFWKSIFEFVKNSYKGTLIQLSPIEGVMVAVKISFSAAIVISMPIIFWQLWLFIAPGLYKNEKKVILPFVFFGSGMFLIGAAFSYYVVFPFIIEYLATFGSDVFAANISASSYVSFFTRLILGFGVAFELPVLAYFLAKVGLITDASLKAYFKYAIVVIFIVAAIITPPDVVSQIFMALPLVGLYGLSILIAKMVNPAPKDNENNNENNNENNTKENTKSES",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design must be able to perform proton motive force dependent protein transmembrane transporter activity in order to contribute to protein transport by the Tat complex, intracellular protein transmembrane transport.2. For general function, the protein need meet that Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin- arginine motif in their signal peptide across membranes; Together with TatB, TatC is part of a receptor directly interacting with Tat signal peptides. The designed protein sequence is ",
    "response#1": "SCRIS",
    "response#2": "EIRF",
    "response#3": "ML"
  },
  {
    "reference": "MQTNRLIGINGGTFDPIHFGHLRPALEVLHALHLDEMRFIPAYQPVHRASPSVSAQQRCEMVQLAIQNQPSFKLDTIELDLGGPSYTVNTLEALKKAEPDASFVLMMGTDAFAKFNQWHDWQGVLNLANIVVTHRPGEPVPRDGEVGQIFMNHWVPNLTEASGQIVDLPVTQLDLSATALRSYLKNGDPVDYLMPENVARYIYEHQLYQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that can modulate the overall flux of metabolites through the deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1, NAD(+) biosynthesis, Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed.2. The protein have enhanced ATP + H(+) + nicotinate beta-D-ribonucleotide = deamido-NAD(+)   + diphosphate. The designed protein sequence is ",
    "response#1": "VMGGTFDPIHYGHLVAASEVADLFDLDEVVFVPSGQPWQKGRQVSAAEHRYLMTVIATASNPRFSVSRVDIDRGGPTYTKDTLADLHALHPDSELYFTTGADALASIMSWQGWEELFELARFVGVSRPGYELRNEHITSLLGQLAKDALTLVEIPALAISSTDCRQH",
    "response#2": "VMGGTFDPIHYGHLVAASEVADLFDLDEVVFVPSGQPWQKGRQVSAAEHRYLMTVIATASNPRFSVSRVDIDRGGPTYTKDTLADLHALHPDSELYFTTGADALASIMSWQGWEELFELARFVGVSRPGYELRNEHITSLLGQLAKDALTLVEIPALAISSTDCRQR",
    "response#3": "VMGGTFDPIHYGHLVAASEVADLFDLDEVVFVPSGQPWQKGRQVSAAEHRYLMTVIATASNPRFSVSRVDIDRGGPTYTKDTLADLHALHPDSELYFTTGADALASIMSWQGWEELFELARFVGVSRPGYELRNEHITSLLGQLAKDALTLVEIPALAISSTDCRQR"
  },
  {
    "reference": "MSEPSEKKQKVATSSLEQLKKAGTHVVADSGDFEAISKYEPQDSTTNPSLILAASKLEKYARFIDAAVEYGRKHGKTDHEKIENAMDKILVEFGTQILKVVPGRVSTEVDARLSFDKKATVKKALHIIKLYKDAGVPKERVLIKIASTWEGIQAARELEVKHGIHCNMTLLFSFTQAVACAEANVTLISPFVGRIMDFYKALSGKDYTAETDPGVLSVKKIYSYYKRHGYATEVMAASFRNLDELKALAGIDNMTLPLNLLEQLYESTDPIENKLNSESAKEEGVEKVSFINDEPHFRYVLNEDQMATEKLSDGIRKFSADIEALYKLVEEKM",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway..2. The engineered protein is expected to adopt a helix-strand-helix-helix-strand-helix-helix-helix-helix-strand-helix-helix-helix-strand-helix-based conformation.3. The designed protein is the optimization of the Schiff-base intermediate with substrate to achieve high catalytic efficiency for D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate =   beta-D-fructose 6-phosphate + D-erythrose 4-phosphate.4. The protein should be optimized for stability and solubility when expressed in nucleus, cytoplasm where it will be involved in cellular response to oxidative stress, pentose-phosphate shunt, non-oxidative branch, carbohydrate metabolic process.5. The protein design should take into account the physiological conditions of the D- glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D- ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3, Carbohydrate degradation, pentose phosphate pathway pathway. The designed protein sequence is ",
    "response#1": "MAXXXSXSXISSXSXXXIXTXQXTXSXPXSXSXXTXNXXT",
    "response#2": "MNTTRQTKVASFENFLKGEHSAKMGMRRLDRLVAELDNPQEAFKCIHITGTNGKGSVTAMIDAILREAGYKTGLYTSPHLVDVRERIQINGVPISESEFVAMLSEVRPVAEYMEQHPTFQEVSVFEILTAMAFYYFRDEKVDVAVVEVGLGGRLDATNVITPLVSVITNIEMDHMAVLGETIEEIAFEKAGIIKPGVPVVSGVLPASALAVIEEKARIKNASLLQVGRDFVVEQSDLGVAGSQFRVWSPRGHFQIRLPGRHSVANAALAIAAVQALRLDMPLDEIRRGLDEAVWPGRMQVVRTSPTVILDGAHNPAGARALAESLREYFPHKRITFIMGVLMDKQYQEIVSELMPLATHFIVTRPNNPRAAKPEELGTIAQKYAGKVEIADTMEQALDQAMEVGGAGEDLVLVAGSLYTVGEARNYWYEEDPLYH",
    "response#3": "MMMMKALISLILLLLLPTLGVTTNPSLSSILPKIQQLEFCSNGSSPRSIGLQSKWIPYVENTPSKIFSSTSTKFNNNKDDDNNNNNNTIIERSSSSSENDQERICVVGLGNPGSKWLDTRHNIGFMAIDKLAKKQSLQFRKWQNLEGEISVGKLSLKFLIPQTYMNLSGEPLRPIIQFFKIPVHSVLVAHDELDLPPGRIKISTKGRGAAGHNGIKSIIQHLGTNEFHRLRVGIGHPGDRSLVANHVLGKFPKAEQDWLNKLLDDAIDCCEILVTKGIATAMNLYNRGCSTLPPSKKKTITKQSKK"
  },
  {
    "reference": "MDSLGQHILVEFLGCDSDVLNDVAAIEKGMIDAALEANATIINTTFHYFSPYGVSGVVVIQESHLAVHTWPEYQYAAVDLFTCGDNVYPWKAFDHLKKIFKAANYSALETRRGSLNLLQRIDFNISEMREDVQNKYVIPEKIQRNVWLTDKDPNIAFSLRYTGDILYQAHSPYQRIKVVDTYAFGKTLLIDNVIMTTEKDEFQYHEMMTHPVMLAHGDVRRVLVIGGGDGGVVREVLRHPQIEQVTMVEIDEKVIEASKLHFPQIASEFDNPKLDLVIGDGIQFVKDAPDASYDLIVVDSTDPIGPAEGLFSVDFYRNCHRILNDNGALIVQGEAPRFNEGTFMAINRTFKDIFGKGKVFAMLFHVPTYPTGLWSAQIAMKNGVDPRVVDREKVAGFVRAHKLKYYNYDVHQASFALPNYVKDMIGED",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein contains novel PABS domains that confer a unique function or activity.2. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor, Proton donor; for catalytic activity, Schiff-base intermediate with substrate; via pyruvic acid, Proton acceptor; for processing activity to enable effective catalysis of putrescine + S-adenosyl 3-(methylsulfanyl)propylamine = H(+) +   S-methyl-5'-thioadenosine + spermidine.3. The designed protein should have adenosylmethionine decarboxylase activity, spermidine synthase activity to facilitate spermidine biosynthetic process.4. For general function, the protein need meet that Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.5. The designed protein should interact with enzymes and substrates within the spermidine biosynthesis, Amine and polyamine biosynthesis pathway to enhance or regulate its activity.6. The designed protein should be able to compete with endogenous proteins or inhibitors for S-methyl-5'-thioadenosine binding. The designed protein sequence is ",
    "response#1": "MWITQEITPYLRKEYTIEAKLLDVRSEHNILEIFKSKDFGEIAMLNRQLLFKNFLHIESELLAHMGGCTKKELKEVLIVDGFDLELAHQLFKYDTHIDFVQADEKILDSFISFFPHFHEVKNNKNFTHAKQLLDLDIKKYDLIFCLQEPDIHRIDGLKRMLKEDGVFISVAKHPLLEHVSMQNALKNMGGVFSVAMPFVAPLRILSNKGYIYASFKTHPLKDLMTPKIEALTSVRYYNEDIHRAAFALDCEVWFTDKNENIALSIRHKGDRIFQERSPYQLVEIFDTFKYGKMLTVDKMVMCSAADDGWFREISDTMWPGQAMTLKVEKVLHHEKSKYQDVLIFKSTTYGNVLVLDNVIQATERDEFAYQEMIAHLALNSHPNPKKVLVIGGGDGGVLREVVKHDSVEEAWLCDIDEAVIRLSKEYLPEMAASYSHPKVKTHIGDGFQFLRDYQNTFDVIITDSSDPEGPAETLFQKEYFQLLNSALTEKGVITTQAESMWIHLPIIKDLKKACSEVFPVAEYSFVTIPTYPTGTIGFMVCSGNQNDR",
    "response#2": "MWITQEITPYLRKEYTIEAKLLDVRSEHNILEIFKSKDFGEIAMLNRQLLFKNFLHIESELLAHMGGCTKKELKEVLIVDGFDLELAHQLFKYDTHIDFVQADEKILDSFISFFPHFHEVKNNKNFTHAKQLLDLDIKKYDLIFCLQEPDIHRIDGLKRMLKEDGVFISVAKHPLLEHVSMQNALKNMGGVFSVAMPFVAPLRILSNKGYIYASFKTHPLKDLMTPKIEALTSVRYYNEDIHRAAFALQARCFACGPAALSALLATATVLADDFERAARHAVYGQDVRVAVRTGVQEVVLTGDADGRPLDLFLDGRLRVRGSDERSYHEALVHPAMGGPHARVLILGGGDGLAAREVLRHPGVRRVDVLEADPGLARLARHDPGLSTLNEHAYGDARVRVLSGDAFHRLRATPSATYDVVISDLPDPGVTASTKLYSQEFYGLLRRVLAPDGRLAVHAGPVDARPRAFWTVDATLRAAGLPAVAYRVGARDTGPGPGSVPGPRRAAALLATATVLADDFERAARHAVYGQDVRVAVRTGVQEVVLTGDADGRPLDLFLDGRLRVRGSDERSYHEALVHPAMGGPHARVLILGGGDGLAAREVLRHPGVRRVDVLEADPGLARLARHDPGLSTLNEHAYGDARVRVLSGDAFHRLRATPSATYDVVISDLPDPGVTASTKLYSQEFYGLLRRVLAPDGRLAVHAGPVDARPRAFWTVDATLRAAGLPAVAYRVGARDTGPGPGSVPGPRRAAALLATATVLADDFERAARHAVYGQDVRVAVRTGVQEVVLTGDADGRPLDLFLDGRLRVRGSDERSYHEALVHPAMGGPHARVLILGGGDGLAAREVLRHPGVRRVDVLEADPGLARLARHDPGLSTLNEHAYGDARVRVLSGDAFHRLRATPSATYDVVISDLPDPGVTASTKLYSQEFYGLLRRVLAPDGRLAVHAGPVDARPRAFWTVDATLRAAGLPAVAYRVGARDTGPGPGSVPGPRRAA",
    "response#3": "MWITQEITPYLRKEYTIEAKLLDVRSEHNILEIFKSKDFGEIAMLNRQLLFKNFLHIESELLAHMGGCTKKELKEVLIVDGFDLELAHQLFKYDTHIDFVQADEKILDSFISFFPHFHEVKNNKNFTHAKQLLDLDIKKYDLIFCLQEPDIHRIDGLKRMLKEDGVFISVAKHPLLEHVSMQNALKNMGGVFSVAMPFVAPLRILSNKGYIYASFKTHPLKDLMTPKIEALTSVRYYNEDIHRAAFALSHAHAIYATPGFAERALAELWYTEEQTQHVRLSFKVKEHLFSEQSEFQKVDVLDTYEFGKLMTLDGLVMVTEKDEFIYHDMIVHVPMAVNPNIKNVLIIGGGDGGTARELMRYQSIKHVDMVEIDKMVCDVARDYFPTISSELENPRVSLYYEDGVAFIKDKENKYDLIIIDSTDPVGPGEGLFSQEFYTNCFKALTEQGILVNQNESPVYEQFAREAIRANSKLKKIFPIVEVYQAQIPTYPSGYWLFGFASDSSPTLSEPLSAGLRRLWELGEVRWQGDTGFQRVLIADTSHGVTLFCDDERQSAESSQLVYHEALFVPAALLAERLDSVLVIGSSEGVVSQLAVSAGAAVVDHVDIDAECVRACARHLPYGYRVEELAAAERGDGPVRMHYADGAAFVEHADRRRDVIVVDLPDERPDDAGAQHNRLYGTEFLRRCAELLTPGGVLVSQAGCPTLWRNETLRTAWRRFGAVFDTVAYHGSDEHEWAFLLGGREPVADPTALMVD"
  },
  {
    "reference": "MFEIAKGISNSLAKKSVGAKVDGKNVDMSYILDHDAEVEFIDIDSPEGEDIVRHSTAHLMAQAVLRLYPDTKVTIGPVIENGFYYDFDPVEQFTEEDLEKIEAEMKRIVKENIKLEKYVLPRDEAIEYFRDVDKNKYKVEVVEGIPQGEQVSFYKQGDFTDLCRGTHVPSTGYLKAFKLRTVAGAYWRGNSKNKMLQRIYGYSFSNEERLKHHLKLMEEAEKRDHRKLGKELELFFLSEYGPGFPFFLPKGMIVRNVLIDLWRREHEKAGYQQLETPIMLNKELWEISGHWFNYRENMYTSEIDELEFAIKPMNCPGGVLAFKHQLHSYKDLPARLAELGRVHRHEFSGALHGLMRVRSFTQDDSHIFMTPDQVQDEIIGVVNLIDKFYSKLFGFEYEIELSTKPEKAIGSQEIWDMAEAALAGALDKLGRKYKINPGDGAFYGPKLDFKIKDAIGRMWQCGTIQLDFNLPERFDVTYIGEDGEKHRPVMLHRVIYGSIERFIGILIEHYAGAFPMWLAPVQVKVLTLNDECIPYAKEIMNKLEELGIRAELDDRNETIGYKIREANGKYKIPMQLIIGKNEVENKEVNIRRFGSKDQFSKLLDEFYTYVVDEATIKFDK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding.2. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.3. The presence of TGS domains is critical for the interaction of the designed protein with its intended targets.4. The ideal protein would be one that plays a critical role in threonyl-tRNA aminoacylation through tRNA binding, metal ion binding within cytosol.5. The protein must exhibit the following characteristics: Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). The designed protein sequence is ",
    "response#1": "MEMDNEELVLRPMNCPHHMMVFKNQLWSYRNLPVRIAELGTMHRHEMSGALAGLQRVRAMTLNDAHIFARPDQLKEEFIRVVELVQHVYKDFGIDDYYFRLSYRDPEDKEKYVDNDEMWEKAQAMLKETMEDMNLEYVEAEGEAAFYGPKLDVQVKTALGKDETLSTIQLDFHLPERFDLTYIGEDGNQHRPVVIHRGVVSTMERFVAFLIEEL",
    "response#2": "MINVYFSDNSCKQFLPGIKGSDIITHLFPELLNKAIAIKINDKSLDLSTEITEDCKFEVISYAIEADKKHLFLRFAACFGQFLMGHDMTFSYRSLPLRMFELTRYSFRREQRGELVGLRRLRAFTMPDMHTFCGDMSSAMEEFRKQYEASISVLRDAGLDLKDYEVAIRFTKDFYENNKTFIEGLVDIVDKPVLIEMWDERFFYFVLKFEFNFVDALDKASALSTVQIDVENAERYDIGYVDAEGNRQRPIILHCSPSGAIERLMYALLEL",
    "response#3": "DHRKLGRELDLFHFQEEAQGMVFWHDKGWSIYNTIEQYIRKKIRKNGYTEVKTPVLVDKSLWEASGHWEKFRDDMFALETDDKTLALKPMNCPCHVQIFKQGIKSYRDLPLRMSEFGLCHRNEASGALHGLMRVRSLVQDDAHIFCAAEQITDETVSFCKLLTEVYKDFGFTDIKVKFSDRPEIRAGSNEVWDKAENALKEAVEQAGFNYTLNPGEGAFYGPKLEFVLTDAIGRQWQCGTLQMDFVLPERLDASYVAASGEKKRPVMLHRAILGSLERFIGILIEEYAGRFRRA"
  },
  {
    "reference": "MAAAALLRRSPAARALLSPALSSRLVASKPHSSAPAPPPPSKAAPNTKTFSIYRWDPDSPSTKPHLKDYKVDLSDCGPMVLDALLKIKNEQDPSLTFRRSCREGICGSCAMNIDGDNGLACLTKISGASSASTISPLPHMFVIKDLVVDMTNFYNQYKSVEPWLKRKDPPPQPGKEIPQTKADRAKLDGMYECILCACCSTSCPSYWWNPEEYLGPAALLHANSNSDDVLCGDCLMWLLAYRLPLWGTLIKPKPNMFMHLQARGYHGVSEKRNLRDHKRRLLAEKYELRGKLYKAVCRDPDLPSDMRDKFRYKLSKLPRNSSMTRLRNRCIFTGRSRAVYKKFRMSRIVFRTLANKVSHVANEVVMIVSVGLYITRSLRMNLQVTLFAYRIISVNAVWWIQDSRDQFTKERLDSINDEFKLYRCHTIKNCTHACPKGLNPAKQIDTIKKLQLDA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).2. A protein that can modulate the overall flux of metabolites through the Carbohydrate metabolism, fumarate from succinate (eukaryal route): step 1/1 pathway, enhancing or suppressing its activity as needed.3. The presence of 4Fe-4S ferredoxin-type domains is critical for the interaction of the designed protein with its intended targets.4. The protein should have electron transfer activity, succinate dehydrogenase (ubiquinone) activity, 4 iron, 4 sulfur cluster binding, fumarate reductase (menaquinone) and be active in ribonucleoprotein complex, respiratory chain complex II, ribosome. The designed protein sequence is ",
    "response#1": "TFQIYRWNPDNPGKPELQNYQIDLKDCGPMVLDALIKIKNEMDPSLTFRRSCREGICGSCAMNIDGCNGLACLTKIQDEASETTITPLPHMFVIKDLVVDMTNFKTFSIYRWDPDSPSTKPHLKDYQVDLSDCGPMVLDALLKIKNEQDPSLTFRRSCREGICGSCAMNIDGDNGLACLTKISSASSASTVSPLPHYRFNPEAPGAKPTVQKFDVDLDQCGTMILDALIKIKNEVDPTLTFRRSCREGICGSCAMNIGGQNTLACICKIDSDTSKSTKIYPLPHFRIYRWSPDNPNQKPFLQSFFVDLPSCGPMVLDALQKIKGEEDSSLSYRRSCREGICGSCAMNIDGVNTVACLKPIDTHTSKPTTITPLPHS",
    "response#2": "FRIYRWSPDNPNQKPFLQSFFVDLPSCGPMVLDALQKIKGEEDSSLSYRRSCREGICGSCAMNIDGVNTVACLKPIDTHTSKPTTITPLPHMFVIKDLVVDLTYFRSHTVAAQGYDPXVHKXEXXYRFNPEAPGAKPTVQKFDVDLDQCGTMILDALIKIKNEVDPTLTFRRSCREGICGSCAMNIGGQNTLACICKIDSDTSKSTKIYPLPHYRFNPEAPGAKPTVQKFDVDLDQCGTMILDALIKIKNEVDPTLTFRRSCREGICGSCAMNIGGQNTLACICKIDSDTSKSTKIYPLPHKYTR",
    "response#3": "FKVYRWNPDHPSQKPFLQSYFLDLTTCGPMVLDALQKIKSEDDSTLSYRRSCREGICGSCAMNIDGTNTVACLKPIDTDTSKATYITPLPHYRFNPEAPGAKPTVQKFDVDLDQCGTMILDALIKIKNEVDPTLTFRRSCREGICGSCAMNIGGQNTLACICKIDSDTSKSTKIYPLPHKKFAIYRWDPDKPGDKPRMQTYEVDLNTCGPMVLDALIKIKNEVDPTLTFRRSCREGICGSCAMNINGGNTLACTVRIDTNLSKVSKIYPLPHKTFSIYRWDPDSPSTKPHLKDYKVDLSDCGPMVLDVLLKIKNEQDPSLTFRRSCREGICGSCAMNIDGDNGLACLTKISSASSASTISPLPHKTFSIYRWDPDSPSTKPHLRDYQVDLSDCGPMVLDALLKIKNEQDPSLTFRRSCREGICGSCAMNIDGDNGLACLTKISSASSASTVSPLPHRL"
  },
  {
    "reference": "MPPKPTYVSGGSVTQTGRSKWRLSYIPEFIWGILNQITFFFSTLIGGTVEPRRRPNNQGGGRRLAGFDGNGNVTGGSGVGGSGPSKGPDNGSNNRRGDMKNILACNSASGSUGPK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in endoplasmic reticulum membrane, Golgi apparatus that is critical for calcium ion transport.2. The protein should have Helical domains to ensure proper insertion into the membrane.3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "SNLWRSDDAGATWSVVKDIPDGKVARLYMHDFDSKRAFAITDGRKHYRTTDQGKTWKTFEVDAAWDTERFDILHFHATDPDRIIFNGLHCLGFLCEEVSLYTIDDFETPAKPLRPDTLGCWWAKSSPVFTTGDSDLDKDRILCIIMGVDSIFSEDRRLVISDDFFKADKEGNIQEFEPNLAGDKPVRGVVNVAAVKRYFMVATTSANTDEMALFISTDTKKWQRAMFPNAHDDHDHKIVQEAYTVLESTNYSIQLNVMTGSKSAPMGIMFTSNYDGTYFSENLEYTNLNMFGHVDFEKIAGIQGIFLVNKVDNGKEVDEKPSTKKKLVSEITFDDGRTFDKVTADGKRIHLHSVTELNNVGRVFSSPAPGLVMANGNTGDYLGDYWEDANLYVSDDAGKTWKKALKGPHKYEFGDQGSILVAVRDSKEEDISEISYSLDHGENWVDEKLPDDLKIQCST",
    "response#2": "TTPDSTGLQFVLIGKAKGAWHVIHIDFEGLHEATCKESDMEEWHARVDKDGEPTCIMGHTQTYPRRKKDAKCFLKKEFKLAEVETKDCDCTDQDYECDYNFERNDKGICVSRGPIPIPEGACKEGDRNGKFLGTSGYRKIPGNTCKDTKETKDKYKDVERSC",
    "response#3": "TTPDSTGLQFVLIGKAKGAWHVIHIDFEGLHEATCKESDMEEWHARVDKDGEPTCIMGHTQTYPRRKKDAKCFLKKEFKLAEVETKDCDCTDQDYECDYNFERNDKGICVSRGPIPIPEGACKEGDRNGKFLGTSGYRKIPGNTCKDTKETKDKYKDVERSC"
  },
  {
    "reference": "MKASELRNYTDEELRNLLEEKKRQLMELRFQLAMGQLKNTSLIKLTKRDIARIKTILRERELGIRR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "MK",
    "response#2": "MSGH",
    "response#3": "PINLDTIQQLAEKAQLTVINPEVLHQHGLVSKRDKCKILGNGLLKTKLEVTAHAFSASASAAIEKLGGKN"
  },
  {
    "reference": "MEEVVVSFGTQDNVFMASSFDQGGSRWKYPGEELERSSSPSVLRSITSKWKEGLLNRKRPFVGRCCHSCTPQSQEKLFNSSIPSLGLRNVIYINETHTRHRGWLARRLCYVLFVLERDVRKDMFARNVVENVLNNSRVEKATIEVASENSPGVMESRIDRKAVSRVRRKARGILQEMVANISPSFIRLTGWVLLKLFNGFFWSIQVHKGQLEMVKKAATEQNVPLVFLPVHKSHIDYLLITFILFCHNIKAPHIAAGNNLNIPIFSTLIRKLGGFFIRRKLDENPSGKKDVLYRSLLHVYTEELLKQHQFLEIYLEGTRSRSGKPSPARAGLLSIIVDTLCSTSISDVLIVPVGISYDRIIEGNYNSEQLGKPKKNESLWSVARGVFRMLRKNYGCVRVDFTQPFSLKEYLDSQRHRQLPAPLTLEQLLVPTIIAAQPDQALFNGEEEGHLSSLDLTDEPWRRQVIGNLAKHVLFTANKSSAVMSTHIVACLLLYRHRQGVYLSKLVEDFFSMKEEVLSRDFDLGFSGNSEDVVMHALHLLGNCVNVTTSSRNTEFFISPATTIPALFELNFYSNGLFHVFITEAIIACSVLAILREQAAETVQSTSEMMVSQERLIRKAAGLSHFLTNEVAVALPCQMVYQVFDDTVGRLIQYGILIVAEEEQEELSPSPTEEPWPKKFPEPLSWRSDEEDEDSDFGEEQRDRYLKVSLSPEHQEFFGFLQRILGPVLEAYSAAAIFLHSLNGPVTEREYTNQLFRFLLTRSEKKVA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipids biosynthesis such as triglycerides, phosphatidic acids and lysophosphatidic acids.2. The protein should be designed to catalyze the following reaction octadecanoyl-CoA + sn-glycerol 3-phosphate = 1-octadecanoyl-   sn-glycero-3-phosphate + CoA.3. The protein should have a specific function in glycerol-3-phosphate metabolic process, CDP-diacylglycerol biosynthetic process, triglyceride biosynthetic process, fatty acid metabolic process and should be localized to mitochondrial outer membrane, mitochondrial membrane in order to carry out this function efficiently.4. The designed protein have Phospholipid/glycerol acyltransferase domains with specific sequence motifs that are crucial for its activity.5. Ideal protein should have a binding affinity for key enzymes in the CDP- diacylglycerol from sn-glycerol 3-phosphate: step 1/3, Phospholipid metabolism pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "YNNEQLGKPKKNESLWSVARGVFRMLRKNYGCVRVDFAQPFSLKEYLDSQSQKPMPAILSLEQALLPAILPSRPNDMVDEVSEAALGDSRDLSTEPFRRQLIANLAEHILFTANKSCAVMSTHIVACLLLYRHREGVDLSKLVEDFFSMKEEVLARDFDLGFSGNSEDVVMHAIHLLGNCVNITNTNQNNEFFISNGVLHVFIKEAIIACSLHAVQSKKYRNGINGISPNMISQEQLVRKAASLCHLLSNEGAISLPCQMLYQVCHEAVERFIQYGILLVAEQDDQEDISPSLTEQQWGKKLPEPLSWRSDEEDEDSDFGEEQRDCYLKVNQSQEHQQYITFLQRLLGPLLEAYSSAAIFIHNFSGPVSETEYLQKLHKYLINRTERE",
    "response#2": "RMRVRGSEQLQLRGPFVIVCNHQASLDLMGMVEVIPERCVPMAKRELQFLGTVGWACWLSGTIFIDRRRRDAAIEVIARTARALRRDNLRVWVFPEGTRNPGKSMLPFKRGAFHLAVQAQVPIVPVVIGICVANHTSPIDIVILANDGCYAMVGQVHGGLMGVIQRSMVRSCPHVWFERSEMKDRHAVAKRLKDHISDKTKLPILIFPEGTCINNTSVMMFKKGSFEFGGTIYPVAIKYYNSEQLGKPKKNESLWSIARGVFRMLRKNYGCVRVDFAQPFSLKEYVNSQSQKPVPAPLSLEQALLPAILPSRPNDTVDESAEASLPNSRDITSEPFRRELISNLAEHILFTADKSCAVMSTHIVACLLLYRHRQGTDLSRLVEDFFSMKEEVLARDFDLGFSGNSDDVVMHAIHLLGNCVNITNTSRNNEFFVTPSITIPAVFELNFYSNGLLHVFIKEAVIACSLHAIQSRRCRNGTSGAAPSLISQEHLVRKAASLCYLLSNEFSVSLPCQVIYQVCHESVERLIQYGILLVAEDDQEDVSPSLTEQQWNKKLPEPISWRSDEEDEDSDFGEEQRDCYLKVSQSQEHQQYITFLQRLLGPLLEAYSSAVIFVHNFSGPVLESEYIQE",
    "response#3": "FWNIQIHRGQIEMVKAATEMNLPLIFLPVHKSHIDYLLLTFILFCHNIKAPYIAAGNNLNIPIFSTLIRKLGGFFIRRKLDQSPDGRKDFLYRALLYVHIEELLRQQQFLEIFLEGTRSRSGKTSGARAGLLSVVVDALFSNATPDVLIIPVGISYDRIIEGHYNSEQLGKPKKNESLWSIARGVFRMLRKNYGCVRVDFAQPFSLKEYVNLPLIFLPVHKSHIDYLLLTFILFCHNIKAPYIAAGNNLNIPIFSTLIRKLGGFFIRRKLDQSPDGRKDFLYRALLYVHIEELLRQQQFLEIFLEGTRSRSGKTSGARAGLLSVVVDALFSNATPDVLIIPVGIGICVANHTSPIDIVILANDGCYAMVGQVHGGLMGVIQRSMVRSCPHVWFERSEMKDRHAVAKRLKDHISDKTKLPILIFPEGTCINNTSVMMFKKGSFEFGGTIYPVAIKYLNNPDSFWNIQIHRGQIEMVKAATEMNLPLIFLPVHKSHIDYLLLTFILFCHNIKAPYIAAGNNLNIPIFSTLIRKLGGFFIRRKLDQSPDGRKDFLYRALLYVHIEELLRQQQFLEIFLEGTRSRSGKTSGARAGLLSVVVDALFSNATPDVLIIPVGISYDRIIEGHYNSEQLGKPKKNESLWSIARGVFRMLRKNYGCVRVDFAQPFSLKEYVPALRETVHYNSEQLGKPKKNESLWSIARGVFRMLRKNYGCVRVDFAQPFSLKEYVNSQSQKPVPAPLSLEQALLPAILPSRPNDTVDESAEASLPNSRDITSEPFRRELISNLAEHILFTADKSCAVMSTHIVACLLLYRHRQGTDLSRLVEDFFSMKEEVLARDFDLGFSGNSDDVVMHAIHLLGNCVNITNTSRNNEFFVTPSITIPAVFELNFYSNGLLHVFIKEAVIACSLHAIQSRRCRNGTSGAAPSLISQEHLVRKAASLCYLLSNEFSVSLPCQVIYQVCHESVERLIQYGILLVAEDDQEDVSPSLTEQQWNKKLPEPISWRSDEEDEDSDFGEEQRDCYLKVSQSQEHQQYITFLQRLLGPLLEAYSSAVIFVHNFSGPVLESEYIQ"
  },
  {
    "reference": "MGGARDVGWVAAGLVLGAGACYCIYRLTRGPRRGGRRLRPSRSAEDLTDGSYDDILNAEQLKKLLYLLESTDDPVITEKALVTLGNNAAFSTNQAIIRELGGIPIVGNKINSLNQSIKEKALNALNNLSVNVENQTKIKIYVPQVCEDVFADPLNSAVQLAGLRLLTNMTVTNDYQHLLSGSVAGLFHLLLLGNGSTKVQVLKLLLNLSENPAMTEGLLSVQVDSSFLSLYDGQVANEILLRALTLFQNINNCLKVEGRLANQIPFAKGSLFFLLYGEECAQKMRALACHHDVDVKEKALAIKPKF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that can perform DNA binding domain binding in endoplasmic reticulum, endoplasmic reticulum membrane to support negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator.2. The protein must exhibit the following characteristics: May play a role in cell survival and cell growth; May suppress the transcriptional activity of p53/TP53 .. The designed protein sequence is ",
    "response#1": "PMFLTVYLSNSEQHFTEVPVTPETICRDVVDLCKEPGENDCHLAEVWCGSERPVADNERMFDVLQRFGSQRNEVRFFLRR",
    "response#2": "LSAEQLEKLLYLLESTDDPIITEKALVTLGNNAAFSTNQAIIRELGGIPIVGSKINSLNQSIKEKALNALNNLSVNVENQTKIKIYVRQVCEDVFADPLNSAVQLAALRLLTNMTVTNDYQHLLSNYITGLLHLLLIGNGSTKVQVLKLLLNLSENPAMTEGLLSAQVDSSFLSLYDGQMANEILLRALTLFQNINNCLRVEGRLANQLPFAKGSLFFLP",
    "response#3": "MPMILTVFLSNNEQILTEVPITPETTCRDVVEFCKEPGEGSCHLAEVWRGNERPIPFDHMMYEHLQKWGPRREEVKFFLRHEDSC"
  },
  {
    "reference": "MGHIRSLAELGLTAQGGRHAQVSGLAVDSREVRKGYLFAALPGTRQHGADFIVYALRQGAAAVLTDVEGLRIAREELEDSEAALIVAEDPRQTLAYTAALWFGAQPETMVAVTGTNGKTSVATFTRQIWERLMFDAVNLGTTGVEGAYSAPLHHTTPEPITLHRLLARCEAAGVTHAAMEASSHGLVQRRLDGVRLMAAGFTNFTQDHLDYHATFEEYFSAKAGLFSRVLPEDGVAVINIDDPRGGDMAAIAAARGQDLISVGRKPGADLHLLAQRPDATGQIMRFAWEDRVHQVRLHLIGGFQAENVLLAAGLVIGTGSDPDRVFETLGAMQTVRGRMQLAAQRENGAAIFVDYAHTPDAVSTAIRALRPHVMGRLIAIVGAGGDRDRSKRPLMGQAAAAHADFVIVTDDNPRSEDPAAIRAAVLAGCPGAVEVADRAEAILRGADMLGPGDALLVCGKGHETGQTVGDDVLPFDDAEQASLAVSVLDGGRV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with ATP binding functions is required to enable cell cycle, regulation of cell shape, peptidoglycan biosynthetic process.2. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.3. The presence of Mur ligase C-terminal domains is critical for the interaction of the designed protein with its intended targets.4. A protein should be able to manipulate the activity of enzymes in the peptidoglycan biosynthesis pathway, either by activating or inhibiting their function.5. The designed protein should have a binding site that is specific to the target ATP ligand. The designed protein sequence is ",
    "response#1": "RDVLAAAGVPIAKGVTVDRLEAAQRHILPPPYVIKPLGEGSSFGVFIVREDQAYPPQELTRSDWAFGNRVLVESYIGGRELTCAVIGEKAHDVIEIKAVGGGWYDYDAKYRKGGSIHILPAELKRNIYQNVQLLSLKAHKALGCRGVSRTDFRYDDRPEGTGELIVLEVNTQPGMTETSLLPEIAAYAGLSFGELVRWMVAE",
    "response#2": "ITGTNGKTTTTYLVEHIARVAGKRTGVIGTVGVRIGDVQEKTAHTTPESPDLQQMFARMRDERCDVVAMEVSSHALDLERTWHTKFAVTAFSNLTQDHLDYHHTFEAYFEAKARLFSKDYPARRVICIDDKWGRELLRRCSAAEDNIITTGFDTAAQIHPIEVNYAPTHTTVTLDVRGSRFSFDYPLVGKFNVENIMCAFGVAHQLGWPLEAILPPPYVIKPLGEGSSFGVFIVREDQAYPPQELTRSDWAFGNRVLVESYIGGRELTCAVIGEKAHDVIEIKAVGGGWYDYDAKYRKGGSIHILPAELKRNIYQNVQLLSLKAHKALGCRGVSRTDFRYDDRPEGTGELIVLEVNTQPGMTETSLLPEIAAYAGLSFGELVRWMGVDRVEQGKSL",
    "response#3": "ITGTNGKTTTSYVCKSLLEAIGGRVGLIGTVAYQIGSEIIPASHTTPGALELQQLLKRMVDGGCSAAVMEVSSHALAQDRVSGCEYDVAVFSNLTQDHLDFHKTMENYFQAKLRLFTGLTGTKKPNKRAIVNSDDSSGARIASLCAVPVWTYGIKGKADLRAEQVQLSLNGTTFMAATPAGTFPVESHLVGEHNVYNMLAAIGVALEMGVSVEILPPPYVIKPLGEGSSFGVFIVREDQAYPPQELTRSDWAFGNRVLVESYIGGRELTCAVIGEKAHDVIEIKAVGGGWYDYDAKYRKGGSIHILPAELKRNIYQNVQLLSLKAHKALGCRGVSRTDFRYDDRPEGTGELIVLEVNTQPGMTETSLLPEIAAYAGLSFGELVRWMGHEKYQEIKGVRHDFDDREVVRELFNG"
  },
  {
    "reference": "MDQREILQKFLHENQDKKIKKEEFANEFLKLKRQSAKYKTDKIYPTTVAERPKNIKKNRYKDILPYDHSLVELSLITSDEDSSYINANFIKGVYGPKAYIATQGPLPTTLLDFWRMIWEHGVLIIVMACMEFEMGKKKCERYWAEPGEKQMQVGPFSISSEAEKRKSDYIIRTLKAKFNSETRTIHQFHYKNWPDHDVPSSIDPILELIWDVRCYQEDDSVPICIHCSAGCGRTGVICAIDYTWMLLKDGIIPENFSIFSLIQEMRTQRPSFVQTQEQYELVYSAVLELFKRQMDVFRDEYSGTEIQAKYSVPEQNSTLEADPYPLSLPKSSIKEATMMNQQNKQSTMVEKGETSSFDFRTSEISEREGFVLHPAKQGISFDFLELNCGCKNADRTMKWETKTFPMAGEPLQKHQSLDLSSILFGACPNSKPINAAQRHFDSKQPITRTKSTPFELIQQRETKELDIKEDFSYLESQPHNSCFVELQAQKLMHASSAELNYSLPRDPKHQICNASNVKQHDPSDFSVHSYMSLAEDPYFSPLPPGSAGSKMSLDLPEKHDGAVFPRSLLPTSSTTIFSYYNSHDSSAPNSLTNFPPLLNQETVVMATSPKTDDEVPPPLPERTPESFIVVEEAGDIPPSAPRPLSSVVKVKTGTSLEWSGTSEFKKFDDSVRLRPTKSVKLRSPNSDLHRDHSSSPPPPLPERTQESFLLADEDCMQAQSVESYSTTCPDTIGNSTSSKQILKTPGKSFTRSRSLKILRNMKKSICNS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The ideal protein would be one that plays a critical role in T cell differentiation, positive regulation of defense response to virus by host, negative regulation of interleukin-6 production through protein tyrosine phosphatase activity, kinase binding, SH3 domain binding within nucleus, cytoplasmic side of plasma membrane, cytoplasm.2. The presence of Tyrosine specific protein phosphatases domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "NKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEFALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVI",
    "response#2": "WQMIVEHKCCVIVMLAREIEAGKKKCEKYWPDAGKQLRFGGISVENRQEVNYSAFRRRVFSVVSEKQEQLTVYQYQFLKWPDHGVPQTTSNLFRMHRAVLKSCQELGNDNPIVL",
    "response#3": "FWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEFALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHR"
  },
  {
    "reference": "MGRLVLLSVSDKTGLIELARQLVEEFEFEIISSGGTAKSLQDAGIPVIKVGDYTGSPEILGGRVKTLHPRIHGGILARRDVPQDLKDLDANNIRPLDLVVVNLYPFEQTIAKDNVTVAQAIEQIDIGGPAMLRATAKNFAHLTVLCNPKYYQPYLEELRQHNGETSLAFRQKMAGETFALTNAYDQAIASYFASLTPEATENPLPTRFSVAGLELQSLRYGENPHQSASWYQSGTQPTGWTAATKLQGKELSYNNLVDLEAARRIIVEFDRSEPSCAILKHTNPCGVAVGTTLAEAYNKAFNADSMSAFGGIVALNQPIDPETAKALTKTFLECVVAPGCDEEAQKILSAKSNVRVLILPDLRSGPKQTVKVIAGGLLVQASDDALDNPETWQVVTEKQPTPEMMAELLFGWKVAKHVKSNAIVVSKNRTTLGVGAGQMNRVGSVKIALETAGEAAKGGYLASDGFFPFDDSVRTAAAAGITGIIQPGGSLKDKDSIKAANELGLVMVLTGMRHFLH",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design must be able to perform phosphoribosylaminoimidazolecarboxamide formyltransferase activity in order to contribute to 'de novo' IMP biosynthetic process.2. The protein contains novel MGS-like domains that confer a unique function or activity.3. The protein should be optimized for activity in IMP biosynthesis via de novo pathway pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "KDFMLRHGIPTARYESFTDTEKAKAFIRSASYQALVVKAAGLAAGKGVVVAANVDEACQAVDEILGDLKYGQAGATLVVEELLEGEEISVLAFTDGKSVRPMLPAQDHKRLGNGDSGPNTGGMGAYCPCPLISQPALELVQRAVLERAVQGLIKERINYQGVLYAGLMLTRDGPRVLEFNCRFGDPETQVILPLLETDLFEVMEACCG",
    "response#2": "AAETLGLPTSPYRFCDSLEELTAAIEGGIGYPCVVKPVMSSSGKGQSKIDGPEGVAAAWEYAMSGSRVSNTRIIVEGFVDFDYEITLLTVRARGADGEIETRFCEPIGHRQVSGDYVESWQPHPMPGTALERARQIAGAVTRDLGGQGIFGVELFVKGDQVWFSEVSPRPHDTGMVTMVTQWQNEADKKWAKDFMLRHGIPTARYESFTDTEKAKAFIRSASYQALVVKAAGLAAGKGVVVAANVDEACQAVDEILGDLKYGQAGATLVVEELLEGEEISVLAFTDGKSVRPMLPAQDHKRLGNGDSGPNTGGMGAYCPCPLISQPALELVQRAVLERAVQGLIKERINYQGVLYAGLMLTRDGPRVLEFNCRFGDPELACPSAVGVVLASAGYPETSTKGCVITGLPTENTPTQLIFHSGLTVNQKNESLTNGGRVLIAIVLDGSLKAAAARATKLAGSISFSGKGAQYRTDIAQKASAVGVVLASAGYPETSTKGCVITGLPTENTPTQLIFHSGLTVNQKNESLTNGGRVLIAIVLDGSLKAAAARATKLAGSISFSGKGAQYRTDIAQKAYTLEDGRTAVGVVLASAGYPETSTKGCVITGLPTENTPTQLIFHSGLTVNQKNESLTNGGRVLIAIVLDGSLKAAAARATKLAGSISFSGKGAQYRTDIAQYADEAXRXGXGXVXDXVXRXXLXXG",
    "response#3": "MTKRALISVSDKTGVTTFAAGLVANGFEIISTGGTRTVLEEAGVPTLAIDDITGFPEMLDGRVKTLHPNIHGGLLAKRGNQAHQKALTEQGIHFIDLVVVNLYPFKETILKPAISEAEAIEMIDIGGPSMLRSAAKNYQDVTAVLTPGQYDGLCAVGVVLASAGYPETSTKGCVITGLPTENTPTQLIFHSGLTVNQKNESLTNGGRVLIAIVLDGSLKAAAARATKLAGSISFSGKGAQYRTDIAQSAVGVVLASAGYPETSTKGCVITGLPTENTPTQLIFHSGLTVNQKNESLTNGGRVLIAIVLDGSLKAAAARATKLAGSISFSGKGAQYRTDIAQKASAVGVVLASAGYPETSTKGCVITGLPTENTPTQLIFHSGLTVNQKNESLTNGGRVLIAIVLDGSLKAAAARATKLAGSISFSGKGAQYRTDIAQKALGLGAAVGVVLASAGYPETSTKGCVITGLPTENTPTQLIFHSGLTVNQKNESLTNGGRVLIAIVLDGSLKAAAARATKLAGSISFSGKGAQYRTDIAQYAGLE"
  },
  {
    "reference": "MRARGIQKNCQRWLTWGTIILGLIMICNTAENLWVTVYYGVPVWKDAETTLFCASDAKAYKAEVHNVWATHACVPTDPNPQEMDLKNVTEKFDMWKNNMVEQMHADIISLWDQSLKPCVKLTPLCVTLNCSNVNVTGPNGTNFNNTAEEMKNCSFNVTTEIRDKKRKAYSLFYKLDIVPMNNNSTEHILINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCKDKNFNGTGLCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIIIRSENISDNAKTIIVQLNQAVPINCTRLNNNTRQSIRIGPGQAFYTTGEIIGDIRKAQCEINGTLWKETLKKVAEKLREHWVNKTINFTQPAGGDLEITTHSFNCGGEFFYCNTTSLFNSTWETNSTWETNSTGXNDTMILPCRIKQIINMWQRAGQAIYAPPIQGLIQCVSNITGLLLTRDGGRINNTTNTAETFRPGGGNMKDNWRSELYKYKVVKIEPLGVAPTRARRRVVEREKRAAIGLGAVFIGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQSNLLRAIEAQQQLLKLTVWGIKQLQARVLAVERYLQDQQLLGIWGCSGKLICTTNVPWNSSWSNKSYKEIWDNMTWMQWDKEIDNYTDTIYRLIEDSQNQQERNEQELLALDKWASLWSWFDITKWLWYIKLFIMIVGGLIGLRIVLVVISLINRVRQGYSPLSFQTHTPSPRDPDRPERIEEEGGGRDRDRSIRLVNGFLTVVWDDLRSLCLFSYHRLR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.2. The designed protein have Human immunodeficiency virus 1 envelope glycoprotein Gp120, Retroviral envelope protein GP41-like domains with specific sequence motifs that are crucial for its activity.3. The designed protein should have at least Helical transmembrane domains to ensure proper localization.4. Incorporating a YXXL motif; contains endocytosis signal, Di-leucine internalization motif motif is critical for the successful design of the protein. The designed protein sequence is ",
    "response#1": "NNGHNITNTDNSSTGEAEMKNCSFYTNPSLGDKRKREYALFYALDIVPIDDNSSSFRLTSCNASVLTQACPKMSFEPIPIHYCAPAGFAILKCNDKRFNGTGPCTNVSTIQCTHGIKPVVSTQLLLNGSLAEKEIVIRSENITDNAKTIIVHLNETVVINCIRPNNNTRKSIPIGPGRAFYATGDIIGDIRQAHCNISERNWTNTLQQVARKLHEQFNKTIIFNQSSGGDPEIVMHTFNCGGEFFYCNTTRLFNSTWNSTQLSNSTWDNNTNEANITLPCRIKQIINMWQEVGKAMYAPPIRGQIRCSSNITGLLLTRDGGIDNKTETFRPEGGNMKNNWRSELYKYKVVRIEPLGVAPTKAKRRVVQREKR",
    "response#2": "GEIKNCSFNITTSIKTKVKDYALFYKFDVVPMNKNNTSYRLISCNTSVITQACPKISFEPIPIHYCTPAGFAILKCNNKTFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSSNFTNNARVIIVQLNESVEINCIRPNNNTRKSIHLGPGKAWYTTGQIIGNIRQAHCNLSSTKWNNTLKQIAEKLREQFGNKTIVFNQSSGGDSEIVMHSFNCGGEFFYCNTSQLFNSAWNSTSTRNDTPGNSTITLPCRIKQIVNMWQEVGKAMYAPPIEGQIRCSSNITGLLLTRDGGNDENRTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR",
    "response#3": "LWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQELVLDNVTENFNMWKNDMVDQMHEDIISLWDESLKPCVKLTPLCVTLNCSNATVS"
  },
  {
    "reference": "MQVLRLDRRHYKSGKIRRAMSSRIPGFYKLSVEERLKKVAEFAGLSDEEVKAVLSQGLPLDVADRMIENVIGTFELPLGIATNFLIDGKDYLIPMAIEEPSVVAAASNAARMARESGGFTTDYTGSLMIGQIQVTKLLNPNAAKFEVLRQKDEIIERANECDPMLVNLGGGCKDIEARVIDTIMGKMLIVHLIVDVKDAMGANAVNTMCEKVAPFIERITGGKVYLRIISNLAAYRLARAKAVFDKDVIGGEEVVEGIMLAYAFAAADPFRCATHNKGIMNGISALMIATGNDFRAIEAGAHSYAAIGGYKPLTTYEVDRKGNLVGTIEIPMAVGVIGGATKVNPLAKISLKILGVNTAEELARVAAALGLAQNFAALRALATEGIQRGHMELHARNLAIMAGATGDEVDRVVEIMVRDGKIRLDYAKEVLERLRS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A key design requirement for the protein is the optimization of the Charge relay system, Proton donor to achieve high catalytic efficiency for (R)-mevalonate + CoA + 2 NADP(+) = (3S)-hydroxy-3-   methylglutaryl-CoA + 2 H(+) + 2 NADPH.2. The designed protein should interact with enzymes and substrates within the Metabolic intermediate biosynthesis pathway to enhance or regulate its activity.3. The protein design must be able to perform hydroxymethylglutaryl-CoA reductase (NADPH) activity in order to contribute to isoprenoid biosynthetic process. The designed protein sequence is ",
    "response#1": "VLEPVRGSCMKHVYDFYKAYFKSEYPLVDGQFSNTCYLGALDACYQRYQAKQRARQAAKTNGTAISNGHQGSFLDTFDYFAFHAPNCKLVAKGYGRLLFNDFKLESGSFDEVPAQVREADFAASLTDKALEKLCVSLTKERFVQRVEPSLTAPTNCGNMYTASVYAGLISLISNVPSDRLQDKRIGMFSYGSGLASTLFSFRVKGDTTEMARKIGLQDRLSARTAVSPEFYDQMCKLREKAYQQRNYTPEGSVESLAPGTYFLVHVDDAYRRKYDML",
    "response#2": "LIFDQSLRGTHMENVNDFYKPDLSSEYPYVDGKLSIECYLRALDKCYLEYKKKFESINDDNKFSMDSFDYVCFHSPYNRLVQKSYARLIYNDFLQNPNNPKYQDLLPFKDLSTGKDSYINSKLDQITLKLSLDDFKTKVNPSTLLSKECGNSYCGSVYSGILSLLSNVNDLNNKKVLVFSYGSGLAASLFSFRINNNKNRNNNNNNNNCFFKTTNDIGKISNIKERLSNRVKVSPEEFTRILDIREKSHQMVGARTPIDTLDYISAGTFYLEKIDEKLIRHYKSL",
    "response#3": "VLEPVRGSCMKHVYDFYKAYFKSEYPLVDGQFSNTCYLGALDACYQRYQAKQRARQAAKTNGTAISNGHQGSFLDTFDYFAFHAPNCKLVAKGYGRLLFNDFKLESGSFDEVPAQVREADFAASLTDKALEKLCVSLTKERFVQRVEPSLTAPTNCGNMYTASVYAGLISLISNVPSDRLQDKRIGMFSYGSGLASTLFSFRVKGDTTEMARKIGLQDRLSARTAVSPEFYDQMCKLREKAYQQRNYTPEGSVESLAPGTYFLVHVDDAYRRKYDMV"
  },
  {
    "reference": "MASVTLKNVCKAYGDVLISKNVDLEIQEGEFVVFVGPSGCGKSTLLRCIAGLEDITSGDLYIGEQRMNDVEPSKRGVGMVFQSYALYPHLNLYDNMSFGLKLSKADKSEIKKRVDHAAEILQLSHLLDRQPKALSGGQRQRVAIGRTLVSQPNVFLLDEPLSNLDAALRVQMRSEITKLQRKLGCTMIYVTHDQVEAMTMADKIVVLDAGFVSQVGKPLELYHYPQNRFVAGFIGSPKMNFMSVFIEGVEKDRVQVQLSNGTTFWIPVDGTTVTRGERMSLGIRPEHLVEAEHGDAKIEGKVMIVEKLGNETQVYMNLKGSDSDVIYRQPDTLDVETGDTLTIGIPAHRCHLFHSDGRACRRLHKEKGVDLPA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The ATP binding site should be located in a region of the protein that is accessible to the ligand.2. The desired protein should be able to perform ATP + D-maltose(out) + H2O = ADP + D-maltose(in) + H(+) +   phosphate3. For general function, the protein need meet that Responsible for energy coupling to the transport system.; Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import4. A protein that localizes to ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing and can perform ATP binding, ABC-type maltose transporter activity with high specificity and efficiency.5. The designed protein must have at least one ABC transporter domain. The designed protein sequence is ",
    "response#1": "LTLDDVTKVYTDEGGGDIVAVEEISLDIDDGEFLVLVGPSGCGKSTTLRMMAGLETVTEGELRLEDRVLNGVSAQDRDIAMVFQSYALYPHKSVRGNMSFGLEESTGLPDDEIRQRVEETTDMLGISDLLDRKPGQLSGGQQQRVALGRAIVRDPEVFLMDEPLSNLDAKLRAEMRTELQRLQGELGVTTVYVTHDQTEAMTMGDRVAVLDDGELQQVGTPLDCYHRPNNLFVAGFRDVSFSVRAGEVVGLGGLVGSGRTEVARLVFGADKMDSGTVELNGKPLHLSSPREAVRARIGLVPEDRKQQGVILDAPIRINTTLAKIRSISRLGFLDAGKERQVAVALGAEMRLKASSVDAPVSSLSGGNQQKVALAKWFHADCDLLILDEPGRVEGEFIVFVGPSGCGKSTILRMIAGLEDVTSGDLLINNIRMNDVPPAKRGVGMVFQSYALYPHLSVADNMSFGMKLAGAKKSDIQQRVKQIAEMLQLAHLLDRRPKALSGGQRQRVAIGRTLVAEPSVFLLDEPLSNLDAALRVQMRIEISRLHKRLQRTMIYVTHDQVEAMTLADRIVVLDGGR",
    "response#2": "VTLSSVYKAFGEAVISRDINLEIDDGEFVVFVGPSGCGKSTLLRMIAGLEDITSGELLIGGKRMNEVPPSERGIGMVFQSYALYPHLSVAENMSFGLKLAGVKKAEIYQRVNQVAEVLQLAHLLDRRPKALSGGQRQRVAIGRTLVSEPDVFLLDEPLSNLDAALRVQMRIEISRLHKRLERTMIYVTHDQVEAMTLADKIVVLDAGNIAQVGKPLELYHYPANRFVAGFL",
    "response#3": "VTLSSVYKAFGEAVISRDINLEIDDGEFVVFVGPSGCGKSTLLRMIAGLEDITSGELLIGGKRMNEVPPSERGIGMVFQSYALYPHLSVAENMSFGLKLAGVKKAEIYQRVNQVAEVLQLAHLLDRRPKALSGGQRQRVAIGRTLVSEPDVFLLDEPLSNLDAALRVQMRIEISRLHKRLERTMIYVTHDQVEAMTLADKIVVLDAGNIAQVR"
  },
  {
    "reference": "MMRTLITTHPLPLLLLPQQLLQPVQFQEVDTDFDFPEEDKKEDFEEYLEQFFSTGPTRPPTKEKVKRRVLIESGMPLNHIEYCTHEIMGKNVYYKHRCVAERYFLLMQYDELQKICYNRFVPCKNGIRKCNRSKGLVEGVYCNLTEAFEIPACKYESLYRKGYVLITCSWQNEMQKRIPHTINDLVEPPEHRSFLSEDGVFVIPP",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Does not exhibit any ribonuclease activity. The designed protein sequence is ",
    "response#1": "DFGFVESYNAVVRVAVDHEKKYLYIYWEYYKNGLTDDKTAEELKEFAETKELIKADSAEPKTIRYFQQHGFNMVGARKYQGSRLQYTKKIKRFRKIICSDRCENTIYELKPLAYATDKLGNIIEDEFTIDPHTLSAIWYALDQ",
    "response#2": "DFGFVESYNAVVRVAVDHEKKYLYIYWEYYKNGLTDDKTAEELKEFAETKELIKADSAEPKTIRYFQQHGFNMVGARKYQGSRLQYTKKIKRFRKIICSDRCENTIYELKPLAYATDKLGNIIEDEFTIDPHTLSAIWYALDG",
    "response#3": "DFGFVESYNAVVRVAVDHEKKYLYIYWEYYKNGLTDDKTAEELKEFAETKELIKADSAEPKTIRYFQQHGFNMVGARKYQGSRLQYTKKIKRFRKIICSDRCENTIYELKPLAYATDKLGNIIEDEFTIDPHTLSAIWYALDE"
  },
  {
    "reference": "MTSGQSKHEIRDMKFDVLVVGEGLAALTLLLHLPPSLKIGVISRNKYDEPSSYWAQGGISAVFSTDDDHDKHIRDTLLAGDGLCDEAAVRQIVCEGGDVLRWLIDQGVPFTREDGEIHLTREGGHSERRVAHVDDMTGRGIMRALQAKVAQLPNVIWIRQYEAVELLSDGQAVSGVIAESLADGEVTVFSAPTVVLAAGGLTGLYQYATNPHASKGEAIAMAWRAGATIENLEFVQFHPTAFQIEGRVISLITEAVRGEGGLLYNVANERFMPGYSSQQELAPRDVVARAIYSEMQAHGTSHVWLDITHQGTAFVEQHFPNLVEITRAHGCDLSQHRVPVSPAAHYTCGGIGADVAGRTNIEGLYAIGEVANCGLHGANRLASNSLLECVVMGKACAQSVTDTAGSASRLRLTLPERIETPFHPNLLADLRAILWNHAGIVRSNTGLEIGLQNMAQLQERHASVLPYGQALRAQNIFDAAHLVLLSAAARKESRGGHFNKDHADKAEAQTTQIPGVPVNFWAAGENASHAAQLAAA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein localizes to the cytoplasm.2. The protein should have a high affinity for FAD. The designed protein sequence is ",
    "response#1": "LMYLTNSTNPEECNPQFVGEMARVATIQNREIGARGFLMYSCRFLLQVIEETVEDPDSLFAKFSPEPRHERWIELDNGPCTGRMYGDWNMKC",
    "response#2": "VVMLATDIGSFTSLTEESSLTEVWMICNTFIDACTSAVCQEGGEVIKRIGDCVTAYFPGNNAESAVAAAQELFTFCRQLREAFVDVVDVRGCVSCGVGLDYSQVVMAQCGSLGLTEYVVAGVSARVMEC",
    "response#3": "VVMLATDIGSFTSLTEESSLTEVWMICNTFIDACTSAVCQEGGEVIKRIGDCVTAYFPGNNAESAVAAAQELFTFCRQLREAFVDVVDVRGCVSCGVGLDYSQVVMAQCGSLGLTEYVVAGVSARVMEC"
  },
  {
    "reference": "MRIGIVGAGGAVGQEIIKILEERKFKVSALSFFGSKRSAGKQIQFKKKRIRIEELRKNSLTNFDLVFFSAGSSISKKFAPIAVSNGSIVIDNSSAFRMNEDTPLVIPEINPESIKGNKPGIISNPNCTTIISLMAIKPLYNLAKVKRVVATSFQSVSGAGNEGINELLNNTKLSLRKNAKSKKNIFERNIAFNVIPKIGNILENLYSEEEMKLHNESRKILLDNKILISATTVRVPVLRSHSISLNIEFRKKISIRKATKALSNFPGLIVMDHPAKGIFPTPIDSSNKDNCIVGRIREDFSSKHGMSIWIVGDQIRKGAALNAVQIAELL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must possess aspartate-semialdehyde dehydrogenase activity to support diaminopimelate biosynthetic process under certain conditions.2. The protein should have a high affinity for NADP(+), substrate.3. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..4. The Acyl-thioester intermediate active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.5. The protein should be optimized for activity in L-threonine biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.6. The designed protein should contain one or more Semialdehyde dehydrogenase NAD-binding domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MSELKPLYDVSKIKRIVVSTYQAVSGGGKEAMAELEEQVKAINEGREVVANILPGASLAKHYQIAFNLIPQIDVFKENLYTKEEMKMIDETKKIMSDDSLRITATTVRVPVYRSHAESVNVEFEDEISVEKAREVLAAFPGVTLTDNPDEQVYPMPLETSGKDDVEVGRIRKDYSIDNGLNFWVCGDQIRDPSEFRAPSLAPLHRKYKIKRIVAATYQSVTGTGVKAVTQMENERKGIKGEMAYAHPIDMNCFPHGGSFQPDTYTTEEQKLIDETRKILEDQSIMVTATVVRIPVVGGHSEAVNVEFEKEYDLQEAISLMSKFPGVVIYDNPAENKYPMPILSHNRDEVFVGRIRRDHSREKCLNLWIVSDNIRELAPLHRKYKIKRIVAATYQSVTGTGVKAVTQMENERKGIKGEMAYAHPIDMNCFPHGGSFQPDTYTTEEQKLIDETRKILEDQSIMVTATVVRIPVVGGHSEAVNVEFEKEYDLQEAISLMSKFPGVVIYDNPAENKYPMPILSHNRDEVFVGRIRRDHSREKCLNLWIVSDNIRPTLPDGLKPLDSEAGLLTLTVTTFQAVSGAGLAGTGELDEQVRKVGDSAPALAITGTAVEFPPAKKFPTTIAYNVIPFAGSLVDDGRGETDEEQKLRNEARKILELPDLKVTATCVRVPVYTGHSLSISAGFSKPISPEDARRLLIKAPGVELADIPTPLMAAGVDPTFVGRIRRDSTLDHGLSLFLSGDNLREIPATFVQGE",
    "response#2": "MKVAIVGVSGAVGQEFLRVLEERNFPLDELILFGSSRSAGKKYLFREREIEVKLLQHNDDFKGVDIAFTSAGGGTAEEYADTITRYGTLMIDNSSAFRMKENIPLVVPEVNAKDAELKALDREAGLRRIVVSTYQAVSGAGGKGIEELAEQVAKLGDQAVALAFDGRTAADLPATVFPVPMAFNVAPLAGHLVDDETNEEHKLREESRKILELPDLRVAVTCVRVGVFTGHSLSVNAEFDRAITAERARELLAAAPGVEMCDLPNPHQAAGADPCYVGRVRADDTVEHGLAFFVSGDNLRGKKGIIADPNCSTIQMVVALHKQAKIKRIVASTYQSTSGAGKAAMDELYNQTKKIFMNEAKKPEIFPKQIAFNCIPHIGEFMENGSTKEEWKMREETEKILGENVKVTATCVRVPVFIGHAIAVNVEFHDAMTEETAYEIFSNADDYGVLLQDKREDGEYTTQIDVVQEDAVYISRIRKDDTVQHGLNMWIVADNLRKGAATNAVQIAEILVKEYL",
    "response#3": "LKPLDSEAGLLTLTVTTFQAVSGAGLAGTGELDEQVRKVGDSAPALAITGTAVEFPPAKKFPTTIAYNVIPFAGSLVDDGRGETDEEQKLRNEARKILELPDLKVTATCVRVPVYTGHSLSISAGFSKPISPEDARRLLIKAPGVELADIPTPLMAAGVDPTFVGRIRRDSTLDHGLSLFLSGDNLRGRPLWDLAPLHRKYKIKRIVAATYQSVTGTGVKAVTQMENERKGIKGEMAYAHPIDMNCFPHGGSFQPDTYTTEEQKLIDETRKILEDQSIMVTATVVRIPVVGGHSEAVNVEFEKEYDLQEAISLMSKFPGVVIYDNPAENKYPMPILSHNRDEVFVGRIRRDHSREKCLNLWIVSDNIRWVNPSGYGPDLAPLHRKYKIKRIVAATYQSVTGTGVKAVTQMENERKGIKGEMAYAHPIDMNCFPHGGSFQPDTYTTEEQKLIDETRKILEDQSIMVTATVVRIPVVGGHSEAVNVEFEKEYDLQEAISLMSKFPGVVIYDNPAENKYPMPILSHNRDEVFVGRIRRDHSREKCLNLWIVSDNIRWVSPAGYGPEGLAPNYVRPHIVAWHRQGHDVQFALFRDGKEIARGTGLNFDF"
  },
  {
    "reference": "MNNQQKIKCPICGKQNTWRPDNQFRPFCSERCKLIDLGEWASESRKIPGSSIDPESIVTSNNKQDNVDEQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must be able to DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) inhibitor activity. The designed protein sequence is ",
    "response#1": "EKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGKETIGLITYMRTDGVTLSNDAIADIRKLIDKNYGNKYLPINPRIYQSKVKNAQEAHEAIRPTNITYTPDNLKQKLEKDYYKLYELIWQRTIACQMENVIMDLVIANLASENKEYLAKANGSIIAFDGFYKVYRESLDDEDEEDNKMLPPLKAQEHIKTKEVIPNKHFTEPPPRYSEASLVKKLEELGIGRPSTYASILSVLQDRKYVTLEKKRFIPEELGRLVTRLFIAEKPSLARAIADALPKPHKKEQGCIRCANGDIVTWCIGHLLEQVEPDAYDERYKKWNMADLPIIPEQWQLRPRKSSSQQLTVVRKLLKEATQIIHAGDPDREGQLLVDEVIDYCKVPKQ",
    "response#2": "LDMKLVAAVECRTEFDLRVGTLFTRFLTNIYKSKRLVGEKEVVSYGTCQFPTLSFVVDRYVRVRNFRPEPFWSIDLAVTKNGQKVNFSWSRNHLFDRMFVYVIYAQLLEGPQKPRIVGVSTKPTSHYKPLPLTTVDLQKCCSRYFKMLAKAALDAAELLYTAGYISYPRTETDQFPAKLDLKGYITKQTLSLDWGTHATRLLQGSFRPPRGGKHDDKAHPPIYPVKSASLDTLRPDQRKVYEFVVRRFLACCSDDARGLQTKVDLQWRSERFTALGLQVTERNFLDVYPYSDWKSLAQLPEFAEGEEVTPSSCKVKEGKTSPPNYMTEAELIALMDANGIGTDATIADHVEKIAQRNYVTRRKIGKSEVFIPTSLGISLIDAFDAILIDRISLLKPFLRRAMEGFLQKISRGEITKQDVRWQTLEHNGVLFPPPYEPLPSYVKLYYEGKPVDLPPLAEEVAGYFAAMIETDHAKNPVFQQNFFNDFLQVLKEGGGCNVDIKEFSKCDFSKMAAFFEKQREEKKSMSKAEKQRIKEEKEKLEEPFKTCLMDGRRENVGNFRVEPPGLFRGRGAHPKTGKFKRRVFPEDITLNLGEGAPIPPPPEGHQWGGIRHDKTVVWLAMWRENISDTFKYVKG",
    "response#3": "TNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICDREDEIERFKAEEYWDISLKMQNSNNELFTAKLTHVNDQKLKKFSIINEKEAKDLTRKLKLQKFYVEKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGKETIGLITYMRTDGVTLSNDAIADIRKLIDKNYGNKYLPINPRIYQSKVKNAQEAHEAIRPTNITYTPDNLKQKLEKDYYKLYELIWQRTIACQMENVIMDLVIANLASENKEYLAKANGSIIAFDGFYKVYRESLDDEDEEDNKMLPPLKAQEHIKTKEVIPNKHFTEPPPRYSEASLVKKLEELGIGRPSTYASILSVLQDRKYVTLEKKRFIPEELGRLVTVFLVGFFKKYVEYDFTAGLENELDEIAAGKLEWKTSLNNFWSGFTRLFIAEKPSLARAIADALPKPHKKEQGCIRCANGDIVTWCIGHLLEQVEPDAYDERYKKWNMADLPIIPEQWQLRPRKSSSQQLTVVRKLLKEATQIIHAGDPDREGQLLVDEVIDYCKVPKSKKETVQRLLISDLNLSAVKRALQGFFKRVAFNEITKKAIIHAVENPRKLDTNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICDREDEIERFKAEEYWKLDTNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICDREDEIERFKAEEYWDISLKMQNSNNELFTAKLTHVNDQKLKKFSIINEKEAKDLTRKLKLQKFYVEKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGKETIGLITYMRTDGVTLSNDAIADIRKLIDKNYGNKYLPINPRIYQSKVKNAQEAHEAIRPTNITYTPDNLKQKLEKDYYKLYELIWQRTIACQMENVIMDLVIANLASENKEYLAKANGSIIAFDGFYKVYRESLDDEDEEDNKMLPPLKAQEHIKTKEVIPNKHFTEPPPRYSEASLVKKLEELGIGRPSTYASILSVLQDRKYVTLEKKRFIPEELGRLVTVFLVGFFKKYVEYDFTAGLENELDEIAAGKLEWKTS"
  },
  {
    "reference": "MALKDKRILLIIAGGIAAYKSLDLVRRLKERGAQVTPLMTKAAQEFITPLSVGALSGGHVFTDLFSRTEEQDVGHIRLARENDLIVVAPATANLMAKAANGIADDLASAVLLARDCPLLLAPAMNPKMWSAAPTQRNLATLMADGVKTVGPMAGEMAESGEAGTGRMAEPLEIVAAIEGVFDDQPKPLAGRRAIVTSGPTHEPIDPVRYIANRSSGRQGHAIARALAKLGAEVTLVSGPVTIADPDGVKTVHVESAREMLAAVEAALPADIAVMVAAVADWRVAQSAPQKMKKKPGEAPPPLPLEENPDILKTIGHHANRPHLVIGFAAETHDVVAYASDKLARKGADLIVANDVSKEGVMGGTRNQVTLVTREGTESWPDMDKDAVANRISDWIAAQSGKK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. The protein design should take into account the physiological conditions of the coenzyme A biosynthesis pathway. The designed protein sequence is ",
    "response#1": "EELILGVTGSIAAYKAVYLLRELTRGGAHVTVCFSEHAREFVSPLTFRTLSGRPVLTNLFDPLSEAAVEHVALAEQAAAVVVAPATANLLGKAAHGIADDFLTTMLLATRSPVVMAPAMDGGMWTHPAVVANVATLRARGVVVLEPDAGALASGLSARGRLPEVDAIVEAVLGH",
    "response#2": "RVVLGVGAGIAAYKVCELLRRLTESGHDVRVVPTADALRFVGEPTWAALSGHPVSAQVWDDVPAVPHVRLGQDADLVLVAPATADLLARAAHGLADDLLTNTLLTAHCPVVFAPAMHTEMWEHPATRANVETLRSRGAVVVEPASGRLTGKDTGPGRLPGF",
    "response#3": "EVVLGVGAGIAAYKVCELLRLFTESGHRVQVVPTADALRFVGEPTWAALSGRPVATGVWDAVHEVPHVRIGQRADLVFVAPTTADLLAKAAHGLADDLLTNTLLTARCPVVFAPAMHTEMWEHPATQANVATLRSRGAIVLDPAVGRLTGADTGRGRLPDE"
  },
  {
    "reference": "MIQQPTTAKPRISTSSKLNTVLSKNKENVPGKLFKKFKCPSLVISEKRKELPLRKKPRVNYSEYGSVDGKYDSAYVSENVSGLATIKEANRLILNHERRDPSTVIKKQFSVPKPIKGHEDISKLCAHRPPPTLGMKRKVDFIPRPLYDPADEFAIVLYDPTTDADEIIPDIKEVLAEKRKKDELLKNRKGKKEISDSEPESDHDSCVSTDTVASCSTEQSLITSNTSKHRRPNKSLKDLLGIQKEKPPPPPVAVVIDPKLARILRPHQIEGVKFLYKCVTGRIDRCANGCIMADEMGLGKTLQCIALLWTLLKQSPQAGKPTIEKAIITCPSSLVKNWANELVKWLGKDAITPFILDGKSSKQELIMALQQWASVHGRQVTRPVLIASYETLRSYVEHLNNAEIGMLLCDEGHRLKNSDSLTFTALDKLNVQRRVILSGTPIQNDLSEYFSLLNFANPGLLGSRQEFRKNYEIPILKGRDADGTEKDKENGDAKLAELAKIVNRFIIRRTNDILSKYLPVKYEHVVFCNLSEFQLSLYKHFITSPEINKILRGTGSQPLKAIGLLKKICNHPDLLNLTEDLEGCEALFPPGFIPRELRGRDRNIDSSLSGKMLVLERMLYQIKQETDDKIVLISNYTSTLDLFEQLCRARGYKALRLDGTMNVNKRQRLVDTFNDPEKDAFVFLLSSKAGGCGINLIGANRLILFDPDWNPAADQQALARVWRDGQKKDCFVYRFIATGTIEEKIFQRQSHKQSLSSCVVDEAQDVER",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit metal ion binding, ATP binding.2. The protein should have a fast turnover rate for ATP + H2O = ADP + H(+) + phosphate.3. The protein must exhibit the following characteristics: Plays an essential role in homologous recombination (HR) which is a major pathway for repairing DNA double-strand breaks (DSBs), single-stranded DNA (ssDNA) gaps, and stalled or collapsed replication forks; Plays also an essential role in RAD51-mediated synaptic complex formation which consists of three strands encased in a protein filament formed once homology is recognized.4. The designed protein should have a binding site that is specific to the target ATP ligand.5. The protein contains novel Helicase C-terminal, Helicase ATP-binding domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "VNEKRQAYWVCTLIDESEVLEAQAAEAIAEDLRKILPHLRIGLVHGRMKPAEKQDIMQAFKQAEIDLLVATTVIEVGVDVPNASLMIIENAERLGLSQLHQLRGRVGGSGVGAAISDKLGRIGIHNLQDLLFHLPIRYEDRTRITPIHDLRPDAYATIEGLVQTCEVQFGKRPILNVSLSDGTSKIMLRFFNFNAGMKNSLQPGARVKAFGEVKRGRFMAEIHHPEYQIIRDNAPLILEETLTPIYSTTEGIKQNSLRKLTDQALDLVRHRPMRRLLQGDVGSGKTLVAAAATLTALEAGYQVAMMAPTEILAEQLHARFQQWLEPLGLEVGYLVGSRSPRARRETAETLAGGSLRLVIGTQSLFQEGVVFACLGLVIIDEQHRFGVEQRRQLLEKGAMPHLLVMTATPIMVEDGITVSGILLFGRTAYGNAMTRHYISFESMKQFINAKKFLSLQGILNLLATSEEFSVMRVRHNEKKLFKEINLSPLLKYPFLTEKKQSQIIDRVSQKVSLLIQYELGGLEFPSYEGASKLHQTLVQDKFLVFRHCFRLLKCMVDTFIEKSDGTSLKNTLFLLK",
    "response#2": "LLDFMLAAIRAHGNDKVVLISNYTQTLDLFEQLARKRKYGFVRLDGTMSIKKRSKVVDRFNDPESDCFLFMLSSKAGGCGLNLIGANRLFMFDPDWNPANDEQAMARVWRDGQKKPCYIYRLVASGSIEEKILQRQTHKKSLSSTIIDNNESAEKHFQWTKEQEEAIYTSGSDILVAAAAGSGKTAVLVERIIQKLLAEHNPTNIDALLVVTFTNAAAQEMRNRVGAALEQALAANPSSIHLKKQLSLLQRASISTLHSFCLDIVKKNAYILDIDPSFRIADDMEMDLLKQEVLDDLLEEWYGDSNPNQDSFFEVVNRFTSDRSDAEMEELIRSLHTFAMQSPWPNQWLDKLVQTYNIPEEWTEEELPWMNVLRDEVSRQLDAVSQEIDIAYSITQEADGPYHYAKTIEEEKAIIQEALKNMESWKYLQQSMLQVKFGKLSGKKVDCDEEKKTKVKKLRDSYKKRFQKMQEVWFARNLDAHVYDMRVLAPVIKHLVELVKQFQDSFAKVKKEKAVVDFSDLEHYCLQILIDESSTEEEVIPSTVAMQLQRQFTEVLVDEYQDTNMVQETILQLISDRQGSGNMFMVGDVKQSIYRFRHAEPSLFIDKYKRW",
    "response#3": "MAAVPLNNLQEQLQRHSARKLNNQPSLSKPKSLGFTFKKKTSEGDVSVTSVSVVKTPALSDKDVNVSEAFSFTESPLHKPKQQAKIEGFFKHFPGRQQSKGTCSEPSLPATVQTAQDTLCTTPKTPTAKKLPVAVFKKLEFSSSADSLSDWADMDDFDMSASDAFASLAKNPATRVSTAQKMKKTKRNFFKPPPRKANAVKTDLTPPSPECLQVDLTKESEEEEEEEEEAEGADCLSRDVICIDNDSASEELTEKDTQESQSLKAHLGAERGDSEKKSHEDEAVFHSVQNTEYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKGILSTSEELLSKPEEMTTHKSDAGTSKDCDAQQIYTTLVIVESPNKARTIAGFFGKPQMRLVEDSVAYEVPLGDRLLVITASLGHVLDLVTDKGFFGVLDDTYPYLPVYDTIKICRETHEQHTEYEYLKKRCKGKIEDKLEIIKGVREVSYEVDEVFIATDPDAEGEKIGYDLYLLSKPFNREKLAKSLKANGIRAKHYHAYMEPVDRSAAHQKWVSGEIQVIVATVAFGMGIDKPDVRFVIHHSLPKSIENYYQESGRAGRDGTAYGNAMTRHYISFESMKQFINAKKFLSLQGILNLLATSEEFSVMRVRHNEKKLFKEINLSPLLKYPFLTEKKQSQIIDRVSQKVSLLIQYELGGLEFPSYEGASKLHQTLVQDKFLVFRHCFRLLKCMVDTFIEKSDGTSLKNTLFLLSGVGAAISDKLGRIGIHNLQDLLFHLPIRYEDRTRITPIHDLRPDAYATIEGLVQTCEVQFGKRPILNVSLSDGTSKIMLRFFNFNAGMKNSLQPGARVKAFGEVKRGRFMAEIHHPEYQIIRDNAPLILEETLTPIYSTTEGIKQNSLRKLTDQAL"
  },
  {
    "reference": "MVRIIPSRLKSASSLSNVNSAANSRSVSPMRNKGDSASPEGRRETGLILNIVIMRARNLAAKDRNGTSDPYLVLSCGDAKHVTHSVSKTLNPEWNEQCEFPINGVQNLLLDVCAWDKDRFGKDYMGEFDLALEEIFANERVEQSPMWFPLKSKRPGKKTSVVSGEVLLQFTIFDSSNREAAHREIYEKLSVIIKSASDTEPSSKNPTPTLTPILAPVKGPKRVGISPTPPLNRRQAESNDDDDDYDVYDDETPDEPEEAEDPNQPDTIEKRKRRLRIKGLKRKKRDHLYEFNNGSSDVVGIIFLEIRSITDLPPESNFTKTSFDMDPFVVASLGKKTYRTRTIRHNLNPTFNEKMIFQVLGHEQQYSFSFTVIDRDKYSNNDFIASTTLPIKEIIDKAPKAHPETGLYDLQDPPEFTTPEKRSRFARLGISRSASASSLSKFGRPSLSKNPSATSGVTLAAAVAAAPVAAAIPASNPSQAGVDSSNKLSPGLFGPSDPVGAAAAPGSDLNPDSSDDAFFTAVDLPLKMKNLEKWEDKHNPVLYIRAKYMPYPALRQQFWRAMLKQYDADESGEISKVELTTMLDTLGSTLKESTIDSFFQRFPHKATNNEGEEEWDLSMDEAVLCLEDQLEAARSKPAHATIADKLKNALPDAEKVKGLLSIPGKGAEDSTAGSSSLSADSTASVTVASKSADASKATEDSTLSIPELSEPGMEGDVLERGDDLGDNDRGEEHVVMIRECPICHQPRLDKRKDTDIITHMATCASQDW",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The presence of pyruvate is crucial for the protein to function optimally under specific conditions.2. The designed protein should interact with enzymes and substrates within the phosphatidylethanolamine biosynthesis, Phospholipid metabolism pathway to enhance or regulate its activity.3. A protein that contributes to phosphatidylethanolamine biosynthetic process, protein autoprocessing by functioning in Golgi membrane with calcium ion binding.4. A key design requirement for the protein is the optimization of the Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity to achieve high catalytic efficiency for a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-   diacyl-sn-glycero-3-phosphoethanolamine + CO2.5. The designed protein should contain one or more C2 domains that are essential for its function. The designed protein sequence is ",
    "response#1": "LKAYVIQARNLAPKDRGGTSDPYLVVTLGAARQSTPSISKTLNPEWKVCLDLPINGVPLVECVLWDKDRFGKDYMGEFDIAVEDIFANGRPTQEPKWYTRPSPPRLGSNNNSTAALVDQKPLILKVYVIKARNLAPKDRSGTSDPYLVVTLGNAKQSTPSISKNLNPEWKICFDMPLTGVPLLECMCWDKDRFGKDYMGEFDIPIEDIFTNGQIQTQPKWYTLQKGDGTLVVRVLAATDLVAKDRNGLSDPFVVIRYASTRVSSSTVPKSLNPVWDDSAKLEVKVFDSRGFGRERIECVLWDKDRVGREYLGEVSIGLDALRVVIMRARNLAAKDRSGTSDPYLVVTLGDARVVTHSVPKTLNPEWNVIEELPISSVQSLVVGVICWDKDRFGKDYLGEFDLALEEIFQNEVAEQGAPEPQAPELKVYVIKARNLAPKDRSGTSDPYLVVTLGNAKQSTPSISKNLNPEWKICFDMPLTGVPLLECMCWDKDRFGKDYMGEFDIPIEDIFTNGQIQTQPKWYKEDKEEDHTGPTLTIENLLRVQVVGCNDLIAKYRNGHSDPFVVISLLRDRHQTPVMRRTLNPTFTPKDATFDFPIYLSLADKLGVLEFVVWDKDMLKKDYLGEANIPLEDWFRDDNAFSKQKVDADDSRRISKIEVTLVVRVLAATDLVAKDRNGLSDPFVVIRYASTRVSSSTVPKSLNPVWDDSAKLEVKVFDSRGFGRERIECVLWDKDRVGREYLGEVSIGLDNAWGSPQDWA",
    "response#2": "WGGSKQKEFNFSAANDILGIVMLEIQKAHDLPKLKNMTRTGWDMDPFVVISFGKKVFRTRVIRHSLNPTWDEKLLFHVRRYETAFKVQLTVLDWDKLSSNDHVGDASFDVSELLANAPQNNPVMVQIGGTIVMLEIQKAHDLPKLKNMTRTGWDMDPFVVISFGKKVFRTRVIRHSLNPTWDEKLLFHVRRYETAFKVQLTVLDWDKLSSNDHVGDASFDVSELLANAPV",
    "response#3": "WGGSKQKEFNFSAANDILGIVMLEIQKAHDLPKLKNMTRTGWDMDPFVVISFGKKVFRTRVIRHSLNPTWDEKLLFHVRRYETAFKVQLTVLDWDKLSSNDHVGDASFDVSELLANAPQNNPTPVANTAQDLRVVIMRARNLAAKDRSGTSDPYLVVTLGDARVVTHSVPKTLNPEWNVIEELPISSVQSLVVGVICWDKDRFGKDYLGEFDLALEEIFQNEVAEQEPRWYPTSQQPSRATSPSRAGGDTNKQSLILKAYVIQARNLAPKDRGGTSDPYLVVTLGAARQSTPSISKTLNPEWKVCLDLPINGVPLVECVLWDKDRFGKDYMGEFDIAVEDIFANGRPTQEPKWSTQTISD"
  },
  {
    "reference": "MTTRQSPAAAVQGALAALAAGRMIVVVDDPDREDEGDLVVAAECLTAAQMAFMVRHGTGIVCTPMSAERADELRLPPMVAHNTDAHGTAFTVSVDHAGAGTGVSAADRTATVHALAAPGTRPEELRRPGHVFPLRARNGGVLVRAGHTEAAVDLTTLAGLSGVGVITEITADDGTMPRGGDIAAFSRQHGLPLLTIADLVRYRRTTERLVEQVATSVMPTAFGTFRAVAYRSALDRSEHLVLVMGDVAAAGRAPRGALVRVHSECLTGDILGSLRCDCGGQLEQALRAIADEGCGALVYLRGHEGRGIGLANKIRAYALQDDGLDTVDANTAQGLTADSRSYGVGAQILADLGVTRLRLITNNPAKFGGLDGYGLIVVDRVALPAVETEHNVRYLRTKRERMGHRLELSADAVACSGQG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that can modulate the overall flux of metabolites through the 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway, enhancing or suppressing its activity as needed.2. The designed protein must be able to perform GTP cyclohydrolase II activity under specific conditions. The designed protein sequence is ",
    "response#1": "SSARLPTKYGEFSIHVYESVIDGTQHFALVKGNIHAQEAVPVRVHSECLTGDILGSCRCDCGAQLDMAIRYIAEQGLGVVVYLRGQEGRGIGFGHKIRAYALQDLGYDTVDANLQLGFPIDAREYGMAAQILKDLQLTSVRLITHNPKKFFGLQRLGIQILDRR",
    "response#2": "TETRLPTPFGEFKALAYKSDIESGEHLALVMGDIKNGTPALVRVHSECLTGDVFGSQRCDCGEQLDMAMKMVAGEKRGVILYMRQEGRGIGFHNKLCAYALQDKGMDTVEANLSLGFDADLRDYGIGAQILADLGLKKIRLLTNNPKKVVGIEGYGLKVVETVK",
    "response#3": "ERVVETRLPTEHGDFTAVGFRGDVDGAEHVALVFGDLGDGEDVLVRVHSECLTGDVFGSVRCDCGPQLDAALDRVAEAGRGVVLYMRGHEGRGIGLLHKLQAYQLQDRGLDTVDANLELGLPADARDFGTGAQILYELGVRSMRLLTNNPAKRAGLEGYGLTVSGR"
  },
  {
    "reference": "MNTLLSRANSLFAFTLSVMAALTLGCILTTAFKDRSAPVRLHVSRILLKKVEDFTGPRKKSDLGFITFHISADLEKTFDWNVKQLFLYLSAEYSTKSNAVNQVVLWDKILLRGENPKLNLKDVKSKYFFFDDGHGLKGNRNVTLTLSWQVIPIAGILPLVTGSGRVSVPFPDSYEIATTF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in signal peptidase complex, endoplasmic reticulum that is critical for fat cell differentiation, protein targeting to ER, signal peptide processing.2. The protein should contain a Helical; Signal-anchor for type II membrane protein membrane-spanning region.3. The protein must exhibit the following characteristics: Essential component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum ; Essential for the SPC catalytic activity, possibly by stabilizing and positioning the active center of the complex close to the lumenal surface ..4. The designed protein should contain Lumenal, Cytoplasmic topological domains. The designed protein sequence is ",
    "response#1": "KGLMVITGSESPIVVVLSGSMEPAFHRGDLLFLTNRVEDPIRVGEIVVFRIEGREIPIVHRVLKIHEKQNGHIKFLTKGDNNAVDDRGLYG",
    "response#2": "ATNSASPIVVVLSGSMEPAFQRGDILFLWNRNTFNQVGDVVVYEVEGKQIPIVHRVLRQHNNHADKQFLLTKGDNNAGNDISLYANKKIYLA",
    "response#3": "KGLMVITGSESPIVVVLSGSMEPAFHRGDLLFLTNRVEDPIRVGEIVVFRIEGREIPIVHRVLKIHEKQNGHIKFLTKGDNNAVDDRGLYL"
  },
  {
    "reference": "MANLEKLVEDLSALTVLEAAELSKMLEEKWGVSAAAPVAVAAAPAAGGGEAAPAGEEQTEFTVVLTAGGDKKINVIKEVRGVRPDLGLKEAKDLVEGAPQNVKENVSKQEAEEIKKKLEEAGASVQIK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.2. The ideal protein would be one that plays a critical role in translation through structural constituent of ribosome within ribonucleoprotein complex, ribosome. The designed protein sequence is ",
    "response#1": "MPN",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#3": "MNRDGS"
  },
  {
    "reference": "MEAGGLPLELWRMILAYLHLPDLGRCSLVCRAWYELILSLDSTRWRQLCLGCTECRHPNWPNQPDVEPESWREAFKQHYLASKTWTKNALDLESSICFSLFRRRRERRTLSVGPGREFDSLGSALAMASLYDRIVLFPGVYEEQGEIILKVPVEIVGQGKLGEVALLASIDQHCSTTRLCNLVFTPAWFSPIMYKTTSGHVQFDNCNFENGHIQVHGPGTCQVKFCTFKNTHIFLHNVPLCVLENCEFVGSENNSVTVEGHPSADKNWAYKYLLGLIKSSPTFLPTEDSDFLMSLDLESRDQAWSPKTCDIVIEGSQSPTSPASSSPKPGSKAGSQEAEVGSDGERVAQTPDSSDGGLSPSGEDEDEDQLMYRLSYQVQGPRPVLGGSFLGPPLPGASIQLPSCLVLNSLQQELQKDKEAMALANSVQGCLIRKCLFRDGKGGVFVCSHGRAKMEGNIFRNLTYAVRCIHNSKIIMLRNDIYRCRASGIFLRLEGGGLIAGNNIYHNAEAGVDIRKKSNPLILCNQIHHGLRSGIVVLGNGKGIIRNNQIFSNKEAGIYILYHGNPVVSGNHIFKGRAAGIAVNENGKGLITENVIRENQWGGVDIRRGGIPVLRSNLICFGYSDGVVVGDEGKGLIEGNTIYANKGCGVWMMSSSLPHVTSNHVSYNGLYGVAVFSQKDGSSELPRGHRAQENFSEDGDAILWETELEKEDDPLRRPITIALVESNSINHNGASGLYVQSSEALHVITNVIHANGDRGITVAQSSQP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have ubiquitin-protein transferase activity within cytoplasm, cytosol, ubiquitin ligase complex to regulate protein ubiquitination, apoptotic process.2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.3. Ideal protein should have a binding affinity for key enzymes in the protein ubiquitination, Protein modification pathway to enhance the overall efficiency of the pathway.4. The presence of F-box domains is critical for the interaction of the designed protein with its intended targets.5. For general function, the protein need meet that Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex; The SCF(FBXO10) complex mediates ubiquitination and degradation of BCL2, an antiapoptotic protein, thereby playing a role in apoptosis by controlling the stability of BCL2. The designed protein sequence is ",
    "response#1": "LTTAAPGYTCPLQGSHAICTCCFQPMPDRRAEHEQDSRIAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLTTRGLTWKNMLTESLLALQRGVFILSDYRITGNTVLCYCCGLRSFRELTYQYRQNRRLIN",
    "response#2": "CLNPCDALIVSIHLLMLESGYIPQGTEAKAVSMPQNWRLGGVYKLQYTHPLCEGGSAALTCVPLGNLIVINATLKINSEVRSVKRLQLLPESFICKEESGENVAMIYKDLQKLSRLFKDQLVYPLLDEDEDDEIVMPVPNVRSSVLQKVIEWAEHHRASRDQPPAYWYAYSIRMSLMPEGCILNGTHHSSCQLYWRHWVIRADNEVIDNVNGEAVIGKYPLLQAGEEEFVYESCSSFPTTAGSIDGSFTFL",
    "response#3": "CLNPCDALIVSIHLLMLESGYIPQGTEAKAVSMPQNWRLGGVYKLQYTHPLCEGGSAALTCVPLGNLIVINATLKINSEVRSVKRLQLLPESFICKEESGENVAMIYKDLQKLSRLFKDQLVYPLV"
  },
  {
    "reference": "MVPEKPTAQSKSGIGEPFRAGDAAGLHIPTSEINSTYLDDTFPSLFSTMHQFRENDAVKAYPSGGIEKFGDIRATVQPVSSGSSDTSSSDDSQQLQHAVEYWKDILADGKFVSYPSPPASTQQRSSLSGLAPHAVEQLQLPLPKHSKVPPATLMRAAWALVAGRMTDSESIVFGTNVLDEPILSAVPFRAHIHGHTVSSFIESVQKQEEEVMASPHQLTLLFSGMEQASTLFQTLLLIPSDEEYSNSPQDSGCRTPEPCGFGLVLEIAHTQDRASLKVTARYRSDTLQAFEVKRLLHRFASVMAQLDTVKPDQSVDEIDFMTEQDFQDIWAWNSKAPAAINGFIHDIVKEKALIQPSSTAVYAWDGEFTYAEIDRHSNRLAVTLITGYGVQPGTPIALCFEKSKWMAVSMLGVLKAGAYFVMLDAASAPEQRLRTMVEQVQARLVISSPLNQALSSRICAAVVTLDSQTLRECKYNDDEIDRLLHQQLLTSSSNALAHVIFTSGSTGTPKAIPTTHQSIRSALHHQVAAIHLNTKSRVYDFSSYSFDAAIFNIWATFYAGGCLCVPSEADRKDDLVGSFQRLGANHVIMTPSAAQLLASAPEKVPQLETIMLVGERLTIQDVLPWWNRVCLINSYGPCECTPLGTSNLNPSSPTDLLDIGVGLGQVTWIVDPDDHNRLVPPGLIGELVLEGPSVSQGYLNDPERTAAAFVKDPAWLVERGRHGKVYKTGDLVQYSNEQGRLKYIGRKDAQVKIRGQRVELGEVEHRVQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that is capable of catalyzing reactions within the Secondary metabolite biosynthesis pathway with high efficiency and specificity.2. The designed protein should be optimized for ligase activity, isomerase activity, phosphopantetheine binding activity in order to improve cellular biosynthetic process, organic substance biosynthetic process.3. The protein design should emphasize Basic and acidic residues compositional bias.4. The protein must exhibit the following characteristics: The HICA residue is probably provided by reduction of alpha-ketoisocaproate by the cluster- specific aldo-keto reductase (NECHADRAFT_45914) (Probable).; Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of sansalvamide, a cyclic pentadepsipeptide that shows promising results as potential anti-cancer drug; The nonribosmal peptide synthetase NRPS30 produces sansalvamide by incorporating successively one phenylalanine, one leucine, one alpha-hydroxyisocaproic acid (HICA), one valine and one leucine before sansalvamide is released from by cyclization by the terminal C domain of NRPS30. The designed protein sequence is ",
    "response#1": "NEPIAIIGSSCRFAGGASSPSKLWDLLLKPKDLSKPPPESRFNLEGFYHPNAEQHGNTNIHGSYFLEEDPRCFDTVFFNISPKEAEAIDPQQRILLEVVYEAMEAAGLTLQGLQGSDTSVYAGLMIRDYMDVQVRDPDFFSQYMVTGTSSALNANRISYFFDWHGPSLTVDTACSSSLVAVHQAVQGLRAGESRVACVTGSNLLLGPELFISASNMHMLSSRSKMWDVSAEGYARGDGFASFMLKTLSNAIADGDHIEAIIRETGVNSDGRTKGITLPSPQAQADLIRDTYRRAGLDLSNPNDRCQYFEAHGTGTQAGDPREASAIHDAFFGAEGDDNKTGPKLVVGSIKTIVGHTEGTAGMAGMLKALLAIRHRVIPPNLHFNNLNPSVAPFYDRLTVPTESIPWPVVAPGTPLRASVNSFGFGGTNAHAIVEEAPDASRDSQDAYPCTPLQEGLVSLASKRTGDYIMQQVLELSPDMLNNIDTFKDAWQKAVHAVPVLRTRIVQHEKLGLLQVLLNHQDEGIEWTEAMGLDWYLKSDRQKPMGLGQPLARYALVRDRTGRPKWFVWTVHHALYDGLSLPMILEEVDRTMQGQSVEQARPQAQAFIKYIQQHDSNELKSYWQATLGDSGECVSYPSLPSGLERPIMSNNLIEHQIPRAWVSSSSNETTVSTMLRAAWGLVTSQMTGSDDVVFGATVSGRNAPVVGVESMAFPTIATVPLRLKLNRKDQRVVDYLDQVQRQATEMIPYEQAGLQRIAQLVSPGARQACAFQTLLVVQPKSKTESTKTSQLGEWTTPDQTEWFTTYPLTIEATVSLSQIDVDARFDSRVVEPWMVKGLLERLDFVMQQLGQAGPDINMSDIGI",
    "response#2": "NEPIAIIGSSCRFAGGASSPSKLWDLLLKPKDLSKPPPESRFNLEGFYHPNAEQHGNTNIHGSYFLEEDPRCFDTVFFNISPKEAEAIDPQQRILLEVVYEAMEAAGLTLQGLQGSDTSVYAGLMIRDYMDVQVRDPDFFSQYMVTGTSSALNANRISYFFDWHGPSLTVDTACSSSLVAVHQAVQGLRAGESRVACVTGSNLLLGPELFISASNMHMLSSRSKMWDVSAEGYARGDGFASFMLKTLSNAIADGDHIEAIIRETGVNSDGRTKGITLPSPQAQADLIRDTYRRAGLDLSNPNDRCQYFEAHGTGTQAGDPREASAIHDAFFGAEGDDNKTGPKLVVGSIKTIVGHTEGTAGMAGMLKALLAIRHRVIPPNLHFNNLNPSVAPFYDRLTVPTESIPWPVVAPGTPLRASVNSFGFGGTNAHAIVESYDAPPVARDEPDRVLFYTPRNGQPSQGYDEVNTKIFANSINRLCGWLDSQLGSPAGPRTIAYIGQNDLRYFIMMIASTKLGHRLLLSSPRNSVEGHVSLIKQSGCEFWIASSGLDHHEFLQDLQIPSVEAPELPQLLDPTPVKPYVYQKSWNEGKSDVLALLHTSGSTGLPKLVPVYLETAATVDGFHLMEPTNGKRPTGVEWTGTRQLCAMPLFHVAGICLGLYSAVFFNWTVVLPSVGPIMQHVIEDALDHISLDSAFISPSVLQDISKSPRVLEKLSKLKFITSAGGPIPQSVGDLIHPRVPIMQTMGMTEGQWLASVVTHPDEW",
    "response#3": "NEPIAIIGSSCRFAGGASSPSKLWDLLLKPKDLSKPPPESRFNLEGFYHPNAEQHGNTNIHGSYFLEEDPRCFDTVFFNISPKEAEAIDPQQRILLEVVYEAMEAAGLTLQGLQGSDTSVYAGLMIRDYMDVQVRDPDFFSQYMVTGTSSALNANRISYFFDWHGPSLTVDTACSSSLVAVHQAVQGLRAGESRVACVTGSNLLLGPELFISASNMHMLSSRSKMWDVSAEGYARGDGFASFMLKTLSNAIADGDHIEAIIRETGVNSDGRTKGITLPSPQAQADLIRDTYRRAGLDLSNPNDRCQYFEAHGTGTQAGDPREASAIHDAFFGAEGDDNKTGPKLVVGSIKTIVGHTEGTAGMAGMLKALLAIRHRVIPPNLHFNNLNPSVAPFYDRLTVPTESIPWPVVAPGTPLRASVNSFGFGGTNAHAIVEEAPPNTARDEPDRVLFYTPRNGQPSQGYDEVNTKIFANSINRLCGWLDSQLGSPAGPRTIAYIGQNDLRYFIMMIASTKLGHRLLLSSPRNSVEGHVSLIKQSGCEFWIASSGLDHHEFLQDLQIPSVEAPELPQLLDPTPVKPYVYQKSWNEGKSDVLALLHTSGSTGLPKLVPVYLETAATVDGFHLMEPTNGKRPTGVEWTGTRQLCAMPLFHVAGICLGLYSAVFFNWTVVLPSVGPIMQHVIEDALDHISLDSAFISPSVLQDISKSPRVLEKLSKLKFITSAGGPIPQSVGDLIHPRVPIMQTMGMTEGQWLASVVTHPDEW"
  },
  {
    "reference": "MGLEAVIDEITEKGRKEAEAIRKESETEVAQVLAAANVKSDQIKTAVDEDVARQEARIINQEVSAANLLVKRSQLNAEKSLLDQVYAAVLLEVRGASPDFHRAALSSLLQQARAEIGEGMIHASARDLPMVQTLLKEGSYPGFVAGSPVDIDGGIIAESRDGLLQVDYSYRTFLDRVWESGLKNASDILFG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein with proton-transporting ATP synthase activity, rotational mechanism, ATP binding, proton-transporting ATPase activity, rotational mechanism is needed to enhance proton motive force-driven plasma membrane ATP synthesis in a specific context. The designed protein sequence is ",
    "response#1": "GWLKENINEDWIKLRNMAMELLKEEDRLQEIVKLVGEDVLPDNQRLILEVARLIKIGFLQQNAFSKVDRFATPEKQYWMLKIIMFLYEKARPLIKNP",
    "response#2": "GQRVFDTFFPLGMGGTAAIPGGFGAGKTMTQHQLAKWSSADIIVYVGCGERGNEMTDVLEEFPKLEDPSTGKSMMERTVLIANTSNMPVAAREASIYTGITIAEFYRDMGYNVALMADSTSRWAEALREISGRLEEMPAEEGFPAYLPSRLAEFYERAGYVKTLGQDREGSISLIGAVSPPGGDFSEPVTQNTKRYVRCFWALDKSLASARHFPAVNWLESYDYADRIDGWLKENINEDWIKLRNMAMELLKEEDRLQEIVKLVGEDVLPDNQRLILEVARLIKIGFLQQNAFSKVDRFATPEKQYWMLKIIMFLYEKARPLIKNRGGKIGLMGGAGVGKTVIIQEMIRRVAKEFGGVSVFAGVGERTREGNDLFLEMTEAGVIEDTALVFGQMDEPPGTRLRVALGALTMAEYFRDVQKQDVLLFIDNIFRFTQAGSEVSTLLGRMPSAVGYQPTLADEMGALQERITSTRGHSITSLQAIYVPADDLTDPAPATTFTHLDANTVLDRAISDLGIYPAVSPLDSNSRILDARYIGQEHYDTAREVQRILQRYKDLQDIIAILGIDELSEEDKILVNRARRIQRFLSQPE",
    "response#3": "QGGKAAMFGGAGVGKTVLVMELIHAVVERYEGISVFAGVGERSREGHEMLLDMRTSGVLPRTVLVYGQMNEPPGARWRVPFTALTIAEYFRDERRQNVLLLMDNVFRFVQAGAEVSGLLGRMPSRVGYQPTLATEVASLQERIVSVGGVSVTAIEAVYVPADDFTDPAVTAIAAHLDSMVVLSS"
  },
  {
    "reference": "MEEKVESTTTPDGPCVVSVQETEKWMEEAMRMAKEALENIEVPVGCLMVYNNEVVGKGRNEVNQTKNATRHAEMVAIDQVLDWCHQHGQSPSTVFEHTVLYVTVEPCIMCAAALRLMKIPLVVYGCQNERFGGCGSVLNIASADLPNTGRPFQCIPGYRAEEAVELLKTFYKQENPNAPKSKVRKKDCQKS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Probably participates in deamination of adenosine-34 to inosine in many tRNAs..2. The designed protein must be able to perform tRNA-specific adenosine-34 deaminase activity under specific conditions. The designed protein sequence is ",
    "response#1": "FTTASENRDYAVKPMNCPGHIQIFNSTLHSYRDLPLRLAEFGSCHRNEPSGALHGLMRVRGFTQDDAHIFCTEDQVKDEVADFIVMLYKAYKDFGFNEVLVKLSTRPEKRVGTDEAWDKAEEALAVALRQNNLDFELQPGEGAFYGPKIEFTLKDSLGRLWQCGTIQLDFNLPERLGAEYVDEDNSRKHPVMLHRAIVGSMERFLGILIENW",
    "response#2": "ANPTYTERVLTLEPERYKRLSDIVVSTGFFFPALFTPADPKLLAKPLGSPEKAGALLGEYLRLYRPDAAQAEWEQSVRELAHEAGVKDGKLFMTLRVAVTGREQTPPLFEVLTILGGDEVQRRLELF",
    "response#3": "DHRKLGRQLDFFHMQDEAPGMVFWHPRGWVIWQEVEQYMRNMFREFGYQEVRTPTIMDRVMWEKSGHWQNYHDNMFTTASENRDYAVKPMNCPGHIQIFNSTLHSYRDLPLRLAEFGSCHRNEPSGALHGLMRVRGFTQDDAHIFCTEDQVKDEVADFIVMLYKAYKDFGFNEVLVKLSTRPEKRVGTDEAWDKAEEALAVALRQNNLDFELQPGEGAFYGPKIEFTLKDSLGRLWQCGTIQLDFNLPERLGAEYVDEDNSRKHPVMLHRAIVGSMERFLGILIENYAGAMD"
  },
  {
    "reference": "MAAPQPAAPHPAGGRTVLLIVCGGIAAYKTLELIRLLTADGVRVRTVLTRAGAQFVTPLSLQALTGERVFSDLFSLTDESEMGHIVLSRSGDLLVVCPATADIMARMVTGQADDLASTVLLATDKPVMIAPAMNVRMWEHPATQANLATLTARGVAVVGPETGLMACNETGPGRLAEPARIAEAIFGHFAAGVRSPSLARRHALVTAGPTHEPIDPVRYLANRSSGRQGYEIAAALAAHGARVTLVSGPVDIPPPPGVELRAVETAIQMQDACQDALPCDIAVCTAAVADWRVAQTSGHKMKKAPGAPPPVLTLVPNPDILAQLSLPGPARPALVVGFAAETDDVEQHAGTKRLDKGCDWIVANDVRPETGIMGGTENEITLITADGAERWPRQDKESVARQLARRIAAFFSPDRSVHDRAHRVCHAADCRCRLSTAGAAGIDLLAAVTEPIVVVAGARVLVPTGLCIALPPGYELQVRPRSGLALKHGITLPNSPGTIDEDYRGEIGIILLNTGDAPLTIERGMRIAQAVLAPVVRAAWQEVETLDETTRSSGGFGSTGVSP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein have Flavoprotein domains with specific sequence motifs that are crucial for its activity.2. The protein should be able to bind substrate ligand in a variety of conditions3. The protein design must be able to perform phosphopantothenoylcysteine decarboxylase activity, dUTP diphosphatase activity in order to contribute to dUMP biosynthetic process.4. The protein have enhanced dUTP + H2O = diphosphate + dUMP + H(+).5. The protein design should take into account the physiological conditions of the dUMP biosynthesis, Pyrimidine metabolism pathway.6. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "KRVLLVIGGGVAAYKSLETIRRLXASGIAARVVLTKAAKQFVTPLSAAGLSGDKVYDELFSLTDEAEMGHIXSSRSADLVVVAPATADLMAKMANGLADDLASTLLLATDKPVLIAPAMNVRMWLHPATRRNLATLKGDGVRVVGPNAGEMACGEFGAGRMAEPNEIADAVEKIARELALPAGYDIHSVESGRIPPNGRKSVRTGLAWDVPQSVVGLVFGRSGLALRNWIEVVETCVHPGEDKELVITLVNNGREVFEYEESSRIAQLVL",
    "response#2": "QMPAYATSGSAGLDLRACIEAPITIKPGETHLIPTGLAIHIGNPAYAAVILPRSGMGHKHGIVLGNLVGLIDSDYQGQLMVSTWNRGQAEFVLNPMERLAQLVIVPVLQVGFNIVDDFDSSERGAGGFGSTGRFVTL",
    "response#3": "QMPAYATSGSAGLDLRACIEAPITIKPGETHLIPTGLAIHIGNPAYAAVILPRSGMGHKHGIVLGNLVGLIDSDYQGQLMVSTWNRGQAEFVLNPMERLAQLVIVPVLQVGFNIVDDFDSSERGAGGFGSTLALYR"
  },
  {
    "reference": "MKNVLFVCTGNICRSPMAEGLFRDLVKGKKDIVVSSGGIGAVNGQPPSSYSVDAVSEIGVDIGSIRSRSITGDIVRKADFIFCMTYGHLDSMLLLFPHAAEKIYLVREFENGLSASSREISDPIGCSREVYRRCRDQIARALPSVLDFVLSSPAPGGDGDSGGLARKVFLVADRSPFSPPIREALYGWLLRQGVPFEDCTPRPGKGAEGAASGFIDRVRRVALAVAQGEARLGILIGEAPAKLVDLANRVGGVRAVAVGDAKSAVSAVLDREANVLCLAAKEVKPQRALEILTAWLNAANAENEDEKPTLMEADIVTNPAPSFNPPPSLKESDPAIAALIAKEAGRQFEGIELIASENFTSRAVMEAQGSCLTNKYAEGYPGRRWYGGCEYVDEIEQIAIDRAKELFGAEYVNVQPHSGSQANMAVYFAFIKPGDTILTMDLSHGGHLTHGNKMNFSGRFYNVVHYGVSQETGYIDYDALAEQAAETKPKLITAGASAYPRIIDFARMSEIAKSVGALLFIDMAHIAGLVAAGVHPSPIPHADFVTTTTHKTLRGPRGGLIMAKAQYGKDLDSHVFPGIQGGPLMHVIAAKAVCFGEALKPGFKAYQEQVVRNAKALSEGLKKNGYKILSGGTDNHLMLVDLRPTEITGKEAQETLDKAAITINKNSIPFDTVSPFKGGGIRLGTPAVTTRGMGPDEMFDIANWIDEALKNRTDEAKLAAIAKRVHELTARFPLPY",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must possess pyridoxal phosphate binding to support glycine biosynthetic process from serine, protein dephosphorylation under certain conditions.2. The protein must exhibit the following characteristics: Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.. The designed protein sequence is ",
    "response#1": "LSQFDPAVAEAIQHETERQEYNLELIASENFVSEAVLEAQGSVMTNKYAEGYPGKRYYGGCHHVDVVENLAIERAKELFGAEHANVQPHAGSQANMAVYNAVCQPGDTILGMNLSHGGHLTHGSPVNFSGRFYNVVPYGVSPDTETIDYNEVERLALEHKPKMIVVGASAYPRIIDFPAFRAIADKVGAKVMVDMAHIAGLVAAGVHPNPVPYAEFVTTTTHKTLRGPRGGMILCREEYAKTINSQIFPGIQGGPLMHVIAAKAVAFKEALQPEFKTYQQQIVKNAAKLAECLMAKGFKLTSGGTDNHLMLINFTGTEITGKAAEEALDKAGITVNKNTVPFETRSPFVTSGIRVGTPACTSHGLKETEMEQVAGFIASALDNPHDAERLKQVRDSFAARYPV",
    "response#2": "LSQFDPAVAEAIQHETERQEYNLELIASENFVSEAVLEAQGSVMTNKYAEGYPGKRYYGGCHHVDVVENLAIERAKELFGAEHANVQPHAGSQANMAVYNAVCQPGDTILGMNLSHGGHLTHGSPVNFSGRFYNVVPYGVSPDTETIDYNEVERLALEHKPKMIVVGASAYPRIIDFPAFRAIADKVGAKVMVDMAHIAGLVAAGVHPNPVPYAEFVTTTTHKTLRGPRGGMILCREEYAKTINSQIFPGIQGGPLMHVIAAKAVAFKEALQPEFKTYQQQIVKNAAKLAECLMAKGFKLTSGGTDNHLMLINFTGTEITGKAAEEALDKAGITVNKNTVPFETRSPFVTSGIRVGTPACTSHGLKETEMEQVAGFICDVLDNFENDETLTAKVKEQVAALASKFPLYP",
    "response#3": "EFERIMKEHTNYMRNSINLIASENITSSDVTEAVASDLAHRYAEGQSHERLYEGCQYIDEIEDRVIDLSKKLFNVDYANVQPISGVTANLAAFFGYSDYGDKLMALDVPYGGHISHAKVSAAGIAGLKTISHPFDKDIMNIDIDAMNKKILEEKPKIVLFGGSLFLFPHPVKEAREAADEVGAKIMYDAAHVLGLIAGGQFQQPIAEGADLMMGSTHKSFPGPQGGIILSHKENKEVIDNAVFPGVVSNHHLHHLAGLGIASAEMLEFGQDYAKQIVKNSKALAQSLYERGFDVLCEELGFTESHQLAINVSNIRSASDIAHDLANNDVILNKNLLPGDNVDDSDNPSGLRIGTQEITRRGLKEKEMDEVAEFLATL"
  },
  {
    "reference": "STVLLFSSILLMLKNNLNNEINESFTSMIIMSALLLKSGAAPFHFWFPNMMEGLTWMNALMLMTWQKIAPLMLISYLNIKYLLLISVILSVIIGAIGGLNQTSLRKLMAFSSINHLGWMLSSLMFSESIWLIYFFFYSFLSFVLTFMFNIFKLFHLNQLFSWFVNSKILKFTLFMNFLSLGGLPPFLGFLPKWLVIQQLTLCNQYFLLTLMMMSTLITLFFYLRICYSAFMMNYFENNWIMKMNMISNNTNMYLIMTFFSIFGLFMISLFYFMF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. We need a protein that can perform a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +   NAD(+) with high efficiency.2. The protein should have NADH dehydrogenase (ubiquinone) activity and be active in mitochondrial inner membrane.3. The protein should have Helical domains to ensure proper insertion into the membrane.4. For general function, the protein need meet that Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis The designed protein sequence is ",
    "response#1": "SSHWFMTWLGFEMNMMAIVPVLMKKYSPRSMEAATKYFLTQATASMILMLAIIINLMYSGQWTIANMENHTASMLITIALVMKLGLAPFHFWVPEVTQGIPLSSGLILLTWQKIAPLSLLYQIHSSINMELFLTMSLLSIVIGGWGGLNQTQLRKIMAYSSIGHMGWMMAIMNYNPNLSFLNLLVYILMTSSMFALLIFSSTTSTLSLSLAWNKTPIIATMSLIILLSLGGLPPLTGFMPKWMIIQELTKNNSTILPTLMAISAV",
    "response#2": "LMSVSILCLFFLINPYQIMIYLMIMTLFLLAKGLINNAESFINFFFFDLMSLSMVILTVWISILMIMASKSNNNYKNKIFNFYLLLMMNLLFY",
    "response#3": "STHWFMIWLGFEMNMMAMIPVLMKKYSPRAAEAATKYFLTQATASMILMLALIINLMYSGQWTIMNMTNNMASLLITIALTMKLGLAPFHFWVPEVTQGVSLQAGLILLTWQKIAPLAVMYQIFASINPNLLLTMALLSIMIGGWGGLNQTQLRKIMAYSSIAHMGWMTAIMIYNPNLMLLNLLLYILMTTSMFMMFMYNSTTTTLSLSLTWNKTPLMTVLMMTTLLSLGGLPPLTGFMPKWMIIHELTKNNSVILPTT"
  },
  {
    "reference": "MEDIKSLLPDELSSFVLSLGQPSYRGDQIFSWLHRHLVCSFDDMTNLPLALRQRLKETSYITCLDVRSRNEGKKGAVKYLLETHDKNAVEAVRMEYKYGISACLSTQVGCKMACSFCASGQGGFVRDLTASEIVDEALKIQEDVALFGANVGNIVLMGTGEPLDNYEASVKALRLLNAKCGLGISFRRMTVSTCGLTPGIRRLAEEEIPVTLSISLHAPTDDVRQVLMPINRRYPIMELMDAAAFYRKRTGRRVTFEYALIKDVNDSDLHARQLVDLLQGQAGHVNLIPLNPIDEKEYARPTTRRINRFHKILQDASIPVTVRRDLGLDVNAACGQLRRRYCR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs..2. A key design requirement for the protein is the optimization of the S-methylcysteine intermediate to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.3. The designed protein should contain one or more Radical SAM core domains that are essential for its function.4. The ideal protein would be one that plays a critical role in rRNA base methylation through tRNA (adenine-C2-)-methyltransferase activity, tRNA binding within cytoplasm. The designed protein sequence is ",
    "response#1": "QSYGNSVCLSTQAGCNMGCTFCASTLHGMVRNLTQGEILAQALFINEVLNKEEQSINTIVIMGSGEPLANYDNVLNFIRLCHETYTMNLGYRHIAVSTAGIVPMIQELAKEDLPITLSISLHAPNNVIRSKLMPINDKFDIHAVVAAGKHYANITKRRVTYEYILIAGENDQAEHAEELSRLLRGQMANVNLIPINPVVERGLKRPSLNQIKVFEEILNNNHIAVTVRREMGNE",
    "response#2": "SSQAGCGMNCPFCATGQAGLTRNLSTGEIVDQVVAAARLLAEGGVGGSPVRLSNVVFMGMGEPLANYKAVMGALRRLTDKDSHGLGLSRRSITVSTVGLVPRIDQLTEENLPVTLAVSLHAPDDELRDTLVPVNTRWKVSEVLEAADRYSRSTGRRYSIEYALIKDINDQTGIANIIQDYSYGNVVCVTSQLGCNMACTFCASGLLRKRRDLTTGEMLIQVLIMNDLLREEGNGERVTHVVVMGTGEPFDNYDNVLNFIRLINHPYGLEIGARHLTISTCGIPDKIKKFAHEGLQVNLAISLHAANNKTRSRLMKINRTYPLEALMEAIKYYEKMTNRRVTYEYILLKDVNDSLADADELSDLIRGTNGYVNLIPFNPVEELPYQRTPNNRVSAFLDRLIKRGITTTVRKEFGTEMNAMYRK",
    "response#3": "YPDRATVCVSSQAGCAMACPFCATGQGGLTRNLSTAEIVDQVRAAAAAMRDGELRGHEGGRNPGRLSNVVFMGMGEPLANYRRVLAAVRRITAPPPEGLGLSQRSVTVSTVGLVPAIRTLAGEGLQVTLAVSLHAPDDELRNVLVPVNTRWPVVEVLAAARYYTDTTGRRVSIEYALIREVNDQPWRADLLGRLLREHLGSLVHVNLIPLNPTPGSEWDASPEPVQREFVRRVQAAGVSCTVRETRGHDIDAACGQLIHGARNAVCVSTQAGCPMACAFCATGEMGLVRNLTAGEIVDQVRHWQRELAQRGERVSHVVFMGMGEPFNNYDATLASTRALIDPEAFGISPRRLTISTCGVVTGIDQLAGEGLPINLAVSLHAPDDPTRARIMPINRKWGVEEVLAAAARYVKRTKRRVTFEYVLLAGVNDSEETARELAALITRHGPTKDAHVNLIPYNPGAGGFRRPSVERMERFAALLQEHGIAATLRISKGQDIDAACGQLRGE"
  },
  {
    "reference": "MKIFSAAQIYKADKLTIKNQQISSEALMERAATGLFQWLHERLRGTSVKIHLFCGIGNNGGDGIALARHLHEHGYNVHVHVVNYSEKRSKDFLINLERLKDQKIWPDFLSEKSTLPKVHESDIVIDAIFGIGLNREPEGWVAHLIKHINSSGAFTLSVDVPSGLYSDRVPENLEAVVEANYVLTIQAPKLSFFLPQTGIFVNQWEIVDIGLDAQYLEETETDYQFITKSDIVPLYIPREKFAHKGSFGHSVIMGGSYGKIGAVVLSTKASLNIGSGLVTAYIPECGYGVLQTAVPEAMVVTDDGDDHVKKIKIPFEPTVIGLGMGLGTETGTVHAVSEFLEKNKTPLVIDADGINILAKQPGLLKKLPPQTILTPHEGELKRLLGTWKDDFDKLKKTMAFSKEHDCIVVVKGAHTIVIYNGKGYINSTGNPGMATAGSGDVLTGTLTGLLSQGYPALHAAIFGVFIHGLAGDIEASQKGYEAVTASSIINNIGNAFMALFSTKQEEEAPSGD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must possess NADPHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding, metal ion binding to support nicotinamide nucleotide metabolic process under certain conditions.2. The protein should be designed to have high stability and activity towards (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.3. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.4. The designed protein should have a high specificity for AMP, (6S)-NADPHX, K(+) binding, with minimal off-target effects.5. The designed protein have YjeF N-terminal domains with specific sequence motifs that are crucial for its activity.6. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. The designed protein sequence is ",
    "response#1": "MREADRRTIHEIGVPSAVLMENAGRQVVAAMSSAFEDLRDRRVAVLAGRGNNGGDGFVIARVLRQRGVEVTVLVLAPLAEVKGDARVNLEILGRLGLPVVEVAGVQQWELHSSEVAGCGVIVDALFGTGLTTPLAGFFETVVEDVNAMGLPVVAVDLPSGMSADTHEVIGPCIDATLTVTLGAPKLPLVLPPARLRVGDLIVADIGIPDEVIGPGDVRLVLRRREKSSHKGDNGRVLIVGGGPFAGAPALSALASLRTGADWVTVAAPRNVASIIASISPNLIVQPLSGSGSGSILTEDDVSLVSSLMQMHDVLVIGMGLGAAEETKRAVKLIIEDEAVKKVVVDADGLYGLDLPLKVSKLKSKLKSKQVIITPHAGEFSKMDAQRPPPDGEERLGFIQEFSLRNRVVTLLKAAHDVISDGKRIKVNATGNAGMTVGGTGDVLSGITGALFATSEDAFEVATAAAFISGSAGDAAFRDKGLSLLATDVVEAIPGILRAIERE",
    "response#2": "MRAADSYTIDELGIPSVVLMEEAGRQVAELCLRLLSERQGRGRAFVWAGRGNNGGDGFVVARRLGQAGYHTEVFLVCPDPDQIQGPARQNLEILRRLNIAVNQAWSEDEITAAAKVTASGDIQVDALLGTGTRGAVTGALAKAIAAMNRAVVPTVAIDLPSGLNADTGEVLGPAVEASYTVTIGRPKIGLVTYPGAEYVGELHVADIGILADAYEGEDVAAWLPIRAADAHKGNFGHLLVVAGREGRAGAAALAARAAVTLGAGLVTVATAHGAVRPVQAQVPEAMVDPLPAGKDGAVSGEGLEGSLGKATALAVGPGLGVSAGPRRLLATILETWVGPLVLDADALTLLAGRLEELRRRTGPTVLTPHPGELGRLLGVPTADVTKNRLQAAQEASRRSGAVVLAKGARTVIVGGGGWSLVNPTGTPGLASGGAGDVLTGVIGALLAQGVPAREAAAAGAWLHGRAAELAGERFPGAVPAGSLVEELSRAELEARLLVVAGREGRAGAAALAARAAVTLGAGLVTVATAHGAVRPVQAQVPEAMVDPLPAGKDGAVSGEGLEGSLGKATALAVGPGLGVSAGPRRLLATILETWVGPLVLDADALTLLAGRLEELRRRTGPTVLTPHPGELGRLLGVPTADVTKNRLQAAQEASRRSGAVVLAKGARTVIVGGGGWSLVNPTGTPGLASGGAGDVLTGVIGALLAQGVPAREAAAAGAWLHGRAAELAGERFPGAVPAGSLVEERLVLRRREKSSHKGDNGRVLIVGGGPFAGAPALSALASLRTGADWVTVAAPRNVASIIASISPNLIVQPLSGSGSGSILTEDDVSLVSSLMQMHDVLVIGMGLGAAEETKRAVKLIIEDEAVKKVVVDADGLYGLDLPLKVSKLKSKLKSKQVIITPHAGEFSKMDAQRPPPDGEERLGFIQEFSLRNRVVTLLKAAHDVISDGKRIKVNATGNAGMTVGGTGDVLSGITGALFATSEDAFEVATAAAFISGSAGDAAFRDKGLSLLATDVVEAIPGI",
    "response#3": "MAAGSLRRLAPRPRTAHKGQLGHLLVVGGDRGLGGAALLAAESALRSGAGLVSLATRPEHVMAALARRPEVMCAGVSSANQLLALADRADVLVAGPGLGQDAWGRGLLSVVAGLESVQVWDADALNMLAGGLVRLPAGCVITPHPGEAARLLGISTAEVQADRPRAVLALAQKFDAVVLLKGAGSLVAAPDGRLALCDRGHPVMAGAGLGDVLAGLIGALLAQGMDAYSATCLAVWLHACAGEGLARGGRGLAASDLIPTIRQLLEEFAPADTYKYSRGHVGVFSGGPAATGAARLSAMAAARSGAGAVTLLSPPASLAVNAVHLTSIILRRIDGPDDAQAFLDQRKPSALVAGPGLGTDNDMAALLATVVQPASAARRVVVLDADALTIIAQEPAKWFGSFALDGAHATILTPHEGEFGRLFPEIHADKSLSKLEKARAAAKSAHSIIVYKGPDTVIANPEGRAAINSNGTPLLATAGSGDVLSGIIAGLAGQGMSPFEAACAGAWLHAEAAHTFGPGLIAEDLPSMLPRVLTALRPPRLADHKFTRGWAMIRAGHPESGGMAGAAILSSRAARRVGAGLVSLVTPAALSDWPGTMVTTKPWADACAEKRATGFLLGPGNGVSPATAAAVLAAAATGAPLVLDADALTSFAGDADRLRRGLAGPCVLTPHEGEFARLFAGTPVDLTQGRLSRARQAARWMGATIVLKGPDTLIAHPDGRVIVQPWAPPALATAGSGDVLAGIILGLMALGISASDAAAAGVWLHAAAGQRAGAHGMIAEDLVDFLPEILG"
  },
  {
    "reference": "MKWYKKIGLLGIVGLTSVLLAACNKSKASQSKEDKVTIEYFNQKKEMDATLKKIIKDFERENPKIHVKMTSVPDAGTVLKTRVLSGDVPDVINIYPQNMDFQEWSKAGYFYNMTGKAYLNHLKNHYANEYKVNQKVYSVPLTANVSGIYYNKTKFKELGLKVPETWDEFVKLVEEIKAKKETPFALAGTEGWTLNGYHQLSLISVTGSANAANKYLRFSQPNSIKTSDKILKEDMVRLNLLADDGNQQKNWKGASYNDALVAFANEKALMTPNGSWALPAIKQQDPKFEIGTFAFPGKKTGNGITVGAGDLALSISAKTKHLKEAEKFVKYMTTARAMQKYYDVDGSPVAVKGVREDKNSPLQPLTKLAFTDKHYVWLGQHWNSEDDFFTATTNYLMTKNAKGLADGLNAFFNPMKADVD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must be able to regulate transmembrane transport. The designed protein sequence is ",
    "response#1": "LNDEGAVKFAEITRTNMGKQLAIMLDGKEQSAPVIQSEIPGGKGSISGSFTYESAQNLANLLKAGALPVNIQIMETRSVDASLGAESIKATKMAAMIALVLVSLFMLAVYRVAGFVADLALCVFGILTAGLMCAIGTTLTLPGIAGFILSLGMAVDANVIIFERIKDEIMEGKRFQDCIDDGFNRAFPAILDGNITTLLITMVLFFFGTGPVRGFAVILTIGVLVSMFTAI",
    "response#2": "QSFWLITGIIFIAFNLRPAITSVGPVISSIRAELHMSNGAAGFLTALPLLSFAVLSPLAPKLGQRLGNERTLWLGLVILLIGVLTRSTGYTAALFFGTALIGVGIAIGNVLLPSLIKHKYPEKPGIMISLYTTSMNIFAALASGVSVPLATQMNGGWKQAFLLWGGLALLALLIWIPQLRHRDTANQTMKLQSSSIWASKMAWYVTIFMGLQSFLFYSSIAWFPEILRSHGIDTATAGWMVSLMQFASLPSTFLTPVLADRVKQQRGIVAALASVYLIGLCGLLAGGSHTLLAIWMIIIGIGQGSSISLALTLIGLRSENAQQAAALSGMSQSFGYLLAAVGPIFVGYLFDQTHSWTMPIVLLIAALIVMGAAGQGAGRDRYR",
    "response#3": "LNDEGAVKFAEITRTNMGKQLAIMLDGKEQSAPVIQSEIPGGKGSISGSFTYESAQNLANLLKAGALPVNIQIMETRSVDASLGAESIKATKMAAMIALVLVSLFMLAVYRVAGFVADLALCVFGILTAGLMCAIGTTLTLPGIAGFILSLGMAVDANVIIFERIKDEIMEGKRFQDCIDDGFNRAFPAILDGNITTLLITMVLFFFGTGPVRGFAVILTIGVLVSMFTALFSNYFFN"
  },
  {
    "reference": "MTIASSHSINNGMNTGKLVNQLSSLPDRNTHNGQEVKYHPENVELNKEKMMDAVRVMLESVGEDPEREGLLKTPKRVAEAMQFLTQGYDQSLEKLVNGAIFDEGHNEMVLVRDIDFFSLCEHHMLPFMGKAHLAYIPNQKVVGLSKLARIVEMFSRRLQVQERLTRQIAEAVQEILDPQGVAVVMEATHMCMVMRGVQKPGSWTVTSAMIGSFQNEQKTREEFLNLIRHQPNFY",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should be able to enhance the efficiency of 7,8-dihydroneopterin 3'-triphosphate biosynthetic process, tetrahydrofolate biosynthetic process. The designed protein sequence is ",
    "response#1": "MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATDLDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFEFYPLVQEMKLELKKPWAPVHLSLDTCSVTIHRRKQR",
    "response#2": "MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATDLDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFEFYPLVQEMKLELKKPWAPVHLSLDTCSVTIHRR",
    "response#3": "MDQLQIKDLEMFAYHGLFPSEKELGQKFIVSAILSYDMTKAATDLDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWAPVHLSLDTCSVTIHRRKQA"
  },
  {
    "reference": "MKLSKLLNSVMVLSVSGNREVDILSVCFDSREVKDGSLFVAVKGGTSDGHDYIGKAVEAGAAAVICEVAPSFSLPVTVVCVEDSHKALGFVASSFYDNPSDKLKLVGITGTNGKTTTVTLLYQLFSGLGYKCGLLSTIANYVYDKRTEATHTTPDPIETNALLAGMAAAGCEYCFMEVSSHSLDQSRVCGLNFTGAIFSNITHDHLDYHKTFQEYIRVKKRFFDNLPPTAFALTNIDDKNGLVMLQNTLAHKYTYSCTSPADFNCRIIEKGLDGMLVRIDSREVWTRFIGEHNAYNLLSVYSSAILLGADKEEVLVQLSSLTSVSGRLEYLKGLNNLTAVVDYAHTPDALENVLKTLKDCAGESEIVAVFGCGGNRDKSKRPEMAQIAARYSDKIIVTSDNPRFEEPESIIEDIKAGFTPKECAGVLFITDRREAIKTALLMSKEGSIILVAGKGHENYQDVKGVKHHFDDKEVISEVFNQNQE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.2. The protein contains novel Mur ligase C-terminal, Mur ligase N-terminal catalytic, Mur ligase central domains that confer a unique function or activity.3. The designed protein should contain at least one Meso-diaminopimelate recognition motif motif to achieve its intended function.4. A protein that contributes to cell division, peptidoglycan biosynthetic process, regulation of cell shape, cell cycle, cell wall organization by functioning in cytoplasm with UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, magnesium ion binding.5. The desired protein should be able to perform ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate6. A protein should be able to manipulate the activity of enzymes in the peptidoglycan biosynthesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "MKLKELLSGLEYELLQGNADIEISGLSFDSRDVQTGNMFFCISGGTHDGHQYASEAIERGAVALIVERELVDENLPEVPYIVVKNTRIAMALASANFYGNPSDKLLLVGVTGTNGKTTTTEIITEILNTAGIATQSIGTLTGVLTTPEAPDLQRQFRSLVNDGIQSVVMEVSSHALSQYRVTGTEFDIGVFTNLSHDHLDFHNDLEDYFKAKCSLFTANQCKTAVVNIDDPFGQRLVKKIDIETCEVSPSSTEVRKTSFTGSSIIWRDQVVELQITGEFNIENALLAAFGCAVLLGLEKEDILKTLSTIPPVEGRMELFSYVKKPTFIIDYAHTPDALKKTLNAIHSHYQRYIWCIFGCGGERDQKKRPIMGAIAEKMSDKVIITNDNPRNEKEKKIIQDILNGCKNKEKILIIPDRKKAISYAYFGAKYHHIILIAGKGHETYQEIKGVKHHFDDREEALKALEGKR",
    "response#2": "MKLKRISNLKMDSRTLTPGDLFIAVPGIKKDGRHFIVQAINKKAAAILCETKKKDKHGIFKYIKNVILIYFFKLSENVSLLANRFYGYPANKCKIIGITGTNGKTTVTQLINQWSTILGTKTATMGTLGNGFYNSLQPTNNTTSSPIFIQLFLSKVLEKQAELVTMEVSSHGLIQHRVKEVPFYIAIFTNLTQDHLDYHENMEKYESAKWLLFSTHKVKKIILNADDQYGKIWLKKLLNFYTVAVTIQNRKQKKYSTKWINATNIEQNNNNSIYITFESSWGTGRISSCLIGHFNVTNLLLSLATCYSLGFNLKEIIKSLSLLKGVAGRMERIDNDLGLDIFIDFAHTPESVYRILSEVRMLTDKRIILIIGCGGNRDELKRPLIGKITTSLADYVIFTTDNPRFENPQNIIDNILQGVVSDNYEVELDRAVAIKKGIDNMTKNDILLILGKGHETYQIIGDKKLDFGDKKIAYEFIKNN",
    "response#3": "MRLQDLLEGIEYIQILGNADIEEISGLSFDSRDVQTGNMFFCISGGTHDGHQYASEAIERGAVALIVERELVPDLKVPQIRVRDSRAALAVASGAFFEEPSRKLKLVGVTGTNGKTTTTEIITEILNTAGIATQSIGTLTGVLTTPEAPDLQRQFRSLVNDGIQSVVMEVSSHALSQYRVTGTEFDIGVFTNLSHDHLDFHNDLEDYFKAKCSLFTANQCKTAVVNIDDPFGQRLVKKIDIETCEVSPSSTEVRKTSFTGSSIIWRDQVVELQITGEFNIENALLAALATGAGIETETVVRGLESVGGVRGRMELFSYVKKPTFIIDYAHTPDALKKTLNAIHSHYQRYIWCIFGCGGERDQKKRPIMGAIAEKMSDKVIITNDNPRNEKEKKIIQDILNGCKNKEKILIIPDRKKAISYAYFGAKYHHIILIAGKGHETYQILADKTIPFDDREVAREALEGRA"
  },
  {
    "reference": "MSNILNKIVATKKIEVANRLKQVSLANQRAQAEANNQDVLLKPRGFIQAIEKKITAGKAAVITEIKKASPSRGILRELFVPTDIAQSYEKHGAACLSVLTDADYFQGCNDYLQQARAACSIPVLRKDFTIDPYQVYEARAIGADAILLIVAYLELNQMKDLEACANELGLDVLVEVHNASELEQALELKTPLLGINNRNLKTFEVTLQNTLSLLSMVPNDKTLVTESGILSHTDVQLMRDHHVNAFLVGEAFMRAADPGAALSELFS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. We are looking for a protein with broad substrate specificity for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+)   = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O.2. The designed protein must be able to perform indole-3-glycerol-phosphate synthase activity under specific conditions.3. The protein should be optimized for activity in Amino-acid biosynthesis, L-tryptophan biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "LDEILKKTREDLKRRKKMLSFELLGRSLSANPYMPRDVIKALKSTPNEPFKLICEIKKASPSKGVIREYFKPVEIAREYEKAGADAFSVLTEPHFFKGDLEFISQIRRYTKMPILRKDFIIDEYQILEALVYGADFVLLIAKALSLKELKKLLEFTHHIGLEALVEVHDKKDLLNATLSGANIIGINHRDLNDFSLHMNLAEELVPLIPNGKIIVAESGLNSREQLVNLNKVGVDAFLIGEHFMRQNDICAAVREL",
    "response#2": "LDEILKKTREDLKRRKKMLSFELLGRSLSANPYMPRDVIKALKSTPNEPFKLICEIKKASPSKGVIREYFKPVEIAREYEKAGADAFSVLTEPHFFKGDLEFISQIRRYTKMPILRKDFIIDEYQILEALVYGADFVLLIAKALSLKELKKLLEFTHHIGLEALVEVHDKKDLLNATLSGANIIGINHRDLNDFSLHMNLAEELVPLIPNGKIIVAESGLNSREQLVNLNKVGVDAFLIGEHFMRQNDICAAVREG",
    "response#3": "TVSLRRAIELCNGEGRRGLIAEYKRRSPSGFSTDEDISSYLSYVKMHRIAGLSILSEPTHFSGSFDDVTLAHRIGVPVLVKDFAPDDHFVESAYNAGGDAVLAILDFLPSEDVERIVRRSADLGMDVIEEYHDRKALKKFVDGDNVILGYNRRDLVSLKTGEDQDMPQYDITILESGINIDNVVHVDMRYSGFLIGTSILKRDGTLEYLS"
  },
  {
    "reference": "MRKIVTIDGPSGVGKGTVAQALARRHGWAYLDSGAVYRLAALFLKRQNLSLAATATQVAALQEMAVDFVMTETVVRVFLNGVAIDDVLRREETGALASQLAVKPEIRAALLDFQRNFAGDAPLIADGRDMGTVVFPEAPLKIFLDATAEIRAERRYKQLIEKGEDVTLAALKKEIAARDARDRNRLVAPLKPASDAVLIDTTDLSVQAVLEKIDALWQAHFFNF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. We are looking for a protein with broad substrate specificity for ATP + dCMP = ADP + dCDP.2. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.3. The designed protein should have CMP kinase activity, dCMP kinase activity, ATP binding within cytoplasm to regulate phosphorylation, pyrimidine nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "VIAVSGQPGSGKTTIAREIARVLGLPLVSSGLLFREMAARMGMDFIEFHKYAETNPDIDKKVDSLAIERAKAGDVVLEGHLTAWIVRPYADVCIYLKASLETRARRVALRDGKSLQDALREVAEREELNRRRYLSIYGIDINDLSIFDLVLDTS",
    "response#2": "MDLNKIAIDGPAGAGKSTVAKKLAKLLGFTYIDTGAMYRAITLKVLRENISLEDEERIVEVARKSDISLDGERVFLDGEDVSEEIRKPIISQKVSVVSQIPEVREILVKKQRKIAEGKNVVMDGRDIGTVVLPDAQFKFFLTASLEERARRRYEELKNKGTEVKYEEVLEEIKKRDSLDSGRKTSPLTIPEGAILIDTSKMTIEEVTDAVIDQIAIDGPAGAGKSTVAKKLAALLGYTYVDSGAMYRALTYWALKNDINIHNVEAIVNLCTSIHITFINQDIAVNNQIVNTEIRSPEVNNNVSHIAKIPEVRKHLVSLQKCIALSQNVIMDGRDIGTHVLPDADIKVFLTASTQERAQRRYIELVEKGIQTSLMEVEQGIIQRDQMDSKRQYAPLTQADDAVVIDSTGLTVEDVTDLIISQIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYLALTNGIEVTDQNRIVDLLAQYPIRFGRDENGQQLVFVGDEDVTLPIRDNQVTNNVSAVAALPLVREELVRLQQDIAQAGGIVMDGRDIGTVVLPQAELKIFLVASVEERALRRFKENTERGIETDLESLKEEIAARDYKDSNREVSPLKAADDAITFDTSGLTIEQVRDMIISK",
    "response#3": "IAIDGHSSSGKSTMAKDLAKEIGYTYIDTGAMYRAVTLYCIQHGFFEGEKIKEEELKASIHDIDISFRLNAETGRPDTYLNGVNVEKEIRGMEVADKVSPVATLGFVRRALVAKQQEMGKAKGIVMDGRDIGTVVFPDAELKLFVTASPEVRAKRRVDELEAKGIPASYEEVLENVKKRDYIDSTREESPLRQADDALVLDNSHMTLEEQKAWLLEQYL"
  },
  {
    "reference": "MSKIIAVTMGDPAGIGPEIIIKSLAEGELSGASAVVVGCVQTMRRILALNVVPTVELKIIDKPADAVFAPGVINIIDEPLEDPQALKPGIVQAQAGDLAYRCIKKATALAMAGEVHAIATAPLNKEALHSAGHLYPGHTELLAKLTNSRDYAMVLYTDKLKVIHVSTHIALRKFLDTLNRDRVETVIEMADVFLKRVGFTHPRIAVAGVNPHAGENGLFGDEEIKIVSPSVEAMKAKGIDVYGPCPPDTVYLQAYEGQYDMVVAMYHDQGHIPLKLLGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKPESMAISIQLAMQLA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must bind to a divalent metal cation in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "LPDAYLSVAEALRHGGYDCGARVDIDWIASDDAEGLLAEGRLHELDGIVVPGGFGFRGIEGKIAAVGYARENDIPFLGLCLGMQCAVVEFARNVCDLAGANSSEFDPHTPHPVIDLMDEQRDVIDMGGTMRLGIYPARLEPGSIVSAAYGEEVIYERHRHRYEVSNKHRPRLEEMGLVCSGTSPDGRLVEFVELPTDKHPFFVATQAHPEFKSRPDRPHPLFAAFVN",
    "response#2": "VRIGLVGKYVNLPDAYLSVAEALRHGGYDCGARVDIDWIASDDAEGLLAEGRLHELDGIVVPGGFGFRGIEGKIAAVGYARENDIPFLGLCLGMQCAVVEFARNVCDLAGANSSEFDPHTPHPVIDLMDEQRDVIDMGGTMRLGIYPARLEPGSIVSAAYGEEVIYERHRHRYEVSNKHRPRLEEMGLVCSGTSPDGRLVEFVELPTDKHPFFVATQAHPEFKSRPDRPHPLFAAFVRIGLVGKYVNLPDAYLSVAEALRHGGYDCGARVDIDWIASDDAEGLLAEGRLHELDGIVVPGGFGFRGIEGKIAAVGYARENDIPFLGLCLGMQCAVVEFARNVCDLAGANSSEFDPHTPHPVIDLMDEQRDVIDMGGTMRLGIYPARLEPGSIVSAAYGEEVIYERHRHRYEVSNKHRPRLEEMGLVCSGTSPDGRLVEFVELPTDKHPFFVATQAHPEFKSRPDRPHPLFAAFVAAN",
    "response#3": "VRIGLVGKYVNLPDAYLSVAEALRHGGYDCGARVDIDWIASDDAEGLLAEGRLHELDGIVVPGGFGFRGIEGKIAAVGYARENDIPFLGLCLGMQCAVVEFARNVCDLAGANSSEFDPHTPHPVIDLMDEQRDVIDMGGTMRLGIYPARLEPGSIVSAAYGEEVIYERHRHRYEVSNKHRPRLEEMGLVCSGTSPDGRLVEFVELPTDKHPFFVATQAHPEFKSRPDRPHPLFAAFIAEH"
  },
  {
    "reference": "MHFKLLSLAALAGLSVASPLNLDERQLGSSSGNDLRDGDCKPVTFIFARASTEPGLLGMSTGPAVCNDLKADASLGGVACQGVGPKYTAGLAENALPQGTSSAAINEAKELFELAASKCPDTRIVAGGYSQGTAVMHGAIPDLSDEIKDKIAGVVLFGDTRNKQDGGQIKNFPKDKIKIYCATGDLVCDGTLVVTAAHFTYVANTGEASKWLEQQLASMPASTSTSSSSSSSSSAPASQTSQSSGLSSWFSGLGN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Catalyzes the hydrolysis of complex carboxylic polyesters found in the cell wall of plants. The designed protein sequence is ",
    "response#1": "PNTKIVTGGYSQF",
    "response#2": "PNTKIVTGGYSQL",
    "response#3": "TTGVPITDLTIDGVTGTVESDAVEVYILCGDGSCSDWTWEGVDITP"
  },
  {
    "reference": "MKVGLVGWRGMVGSVLMQRMQQENDFAGIEPVFFSTSQAGAPAPMNAGTLKRAEDIAELKKLDVILTCQGGDYTKAIHPQLRQAGWQGYWIDAASTLRMEKDAVIILDPVNRGVIDAALQNGQKDFIGGNCTVSLMLMALGGLFRAGQVEWLTSMTYQAASGAGAPNMRELISQMGAIHGSVHERLQDVASAILEIDREVTAMLRSDLLPTQEFGFPLAGSVLPWIDREVEDGQSREEWKGLVETNKILGSKTPIPVDGICVRVGAMRCHSQALTIKLKQDLPIADIEELIKNDNQWVKLIPNTKAATLAGLTPTAVSGTLSVPVGRVRKMKMGPHYLSAFTCGDQLLWGAAEPLRRMLRILLER",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be designed to have high stability and activity towards L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.2. The protein design should aim to mimic or enhance the function of natural proteins involved in 'de novo' L-methionine biosynthetic process, isoleucine biosynthetic process, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "LAGLFEQGWVEWVTSQTYQAASGAGAKNMRELVNQMRIIGENCENLLDTPSTAILDIDRNVINTLRNPAFPTENFGAPLAASLIPWIDRAMENGQTREEWKGISETNKILGREENPVPIDGCCVRVGSMRCHSQAYTIKLNRDVPMADIEDAIKKQNDWVYLVANNKEETLKELTPARVTGTLDIPVGRLRKMNLGPQYLSAFSVGDQLLY",
    "response#2": "LLQEQYGLKRVAYTTYQAVSGSGVKGKQDLADGPSGKAPEAYPHPIYNNVLPHIDVFLEDGYTKEEQKMIDETRKILKDDDLKVTATCVRVPVQDSHSVHMSVTLEKDATVESIQELFSKDERVVLIDDPQNNEYPLAIYSTGKDEIFVGRIRRDETLDNTFHVWCTSDNLLD",
    "response#3": "LKPIFDLAGIRRVVVSTYQAVSGAGREGIEELETQVKAWTRGEELTSKVFPYQIAFNLIPRIDIFQEGDYTKEEWKMVYETQKIFHAPNMAITATTVRVPVLRSHSESINVETDRLVSVSEVREALSKAAGVIVDDDPGNDSYPMPWFSSDKDEVYVGRLRKDFSIAQGFNLWVAGDQIRR"
  },
  {
    "reference": "MVFKKVLLTGTSEESFTAAADDAIDRAEDTLDNVVWAEVVDQGVEIGAVEERTYQTEVQVAFELDGSQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Protects bound flavins against light damage; Trp-36 rapidly quenches the flavin excited state; Promotes the conversion of bound riboflavin to lumichrome. The designed protein sequence is ",
    "response#1": "PLFAGGEIVRGFGRGSKELGIPTANFPLEVVKSLPESLPTGAYYGWANVDNGPVHKMVLSIGWNPFYNNKEKSVETHMLHDFNCDLYGQTLKICIVGYLRPERSFDSLESLIAAIRGDIEQAKAFL",
    "response#2": "PLFAGGEIVRGFGRGSKELGIPTANFPLEVVKSLPESLPTGAYYGWANVDNGPVHKMVLSIGWNPFYNNKEKSVETHMLHDFNCDLYGQTLKICIVGYLRPERSFDSLESLIAAIRGDIEQAKAFR",
    "response#3": "PLFAGGEIVRGFGRGSKELGIPTANFPLEVVKSLPESLPTGAYYGWANVDNGPVHKMVLSIGWNPFYNNKEKSVETHMLHDFNCDLYGQTLKICIVGYLRPERSFDSLESLIAAIRGDIEQAKAFL"
  },
  {
    "reference": "MNFSNFYDLSYSDLSDFISSNFSLEKKKTSMRTNQIWKFVYKKGLRETSKFSNISSELKYNIEKSFNFNRTNIAEKKISKDGTIKWLLKLSDNNLVETVFIPSGKRGTLCVSSQVGCTLNCKFCHTGTQLMVKNLTTHEIINQILVAKDELNDWGSQKKITNIVYMGMGEPFYNYDNVKKSISILRERNGLDYSAKKITVSTAGISNEIMKAADEIGTYLALSLHAPTDELREKIMPINKKFKIKDLIESCSYYSKINKEKIFLEYVLLKDINDTDSCAQQLVKLMSKFPSKLNLIEFNAWPGVGYEPSDSETVQKFYEKIKKSGHIVTLRKSRGEDILGACGQLKTDSEKKRKSL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The S-adenosyl-L-methionine, [4Fe-4S] cluster binding site should be stable and able to withstand changes in environmental conditions.2. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.3. The protein must be engineered to have a cooperative binding mechanism with [4Fe-4S] cluster to ensure proper regulation.4. The designed protein should be involved in rRNA base methylation by localizing to cytoplasm and exhibiting rRNA (adenine-C2-)-methyltransferase activity, rRNA binding, 4 iron, 4 sulfur cluster binding, tRNA binding.5. The designed protein is the optimization of the Proton acceptor, S-methylcysteine intermediate to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "MHTPESLNAWCRDVGMPPFRATQILEWVYAKGVADPDAMSNLGKADRDLLRNQITFLSGAVVRHQRATDGTQKLLVEWDDFGAKAADAERRVSLMPGSDTSRQTECVMIPSLDADERHAPARFTACISSQVGCPVGCRFCASGIGGLDANLSAGRIVEQVWRLSRLEGVRRISNVVFMGMGEPLANLGSVVPAIRTLAAPWGLGISARKITVSTVGLPKAIDKLAEQLDLPVTLALSLHAPTDEIRRELIPWAEYTTIRELLDACRRWFERTGREVTLEYTLLRGVNDQPAHARELARLARTLRANVNLIRYNEVSGVEFERPRDADVRTFQDVLREGGINAHIRASRGRDIAAACGQLRHEQARLRSAA",
    "response#2": "MKTPLLGKTLSELKELVKNMDLPAFTAGQLAYWLYKTNIQSFDEMTNLSKKVRLLFEEQYVIGSIESVKVQVSGDGTKKYLYPTRDSKFVESAYIPDERRNTLCVSTQVGCKMGCLFCMTGKQGFQANLCAGEIVNQLRSLPERNTITNIVFMGMGEPFDNLDAVMQAQEILTSEYGFALSVRKVTVSTIGLVPAMKVFLEKSKCNLAVSLHTPFDEERRKLMPIQHVYPIKEVINTINQFDLGKYRRVSMEYIVFSGLNDTSSHVKELARLLNKTRCRINLIRFHPVPGVPLPGTDEEKLLLFQRALNDKGIITTIRASRGLDIDAACGLLSTKELL",
    "response#3": "MGYKTPLLGKTLSELKELVKNMDLPAFTAGQLAYWLYKTNIQSFDEMTNLSKKVRLLFEEQYVIGSIESVKVQVSGDGTKKYLYPTRDSKFVESAYIPDERRNTLCVSTQVGCKMGCLFCMTGKQGFQANLCAGEIVNQLRSLPERNTITNIVFMGMGEPFDNLDAVMQAQEILTSEYGFALSVRKVTVSTIGLVPAMKVFLEKSKCNLAVSLHTPFDEERRKLMPIQHVYPIKEVINTINQFDLGKYRRVSMEYIVFSGLNDTSSHVKELARLLNKTRCRINLIRFHPVPGVPLPGTDEEKLLLFQRALNDKGIITTIRASRGLDIDAACGLLSTKELKK"
  },
  {
    "reference": "MLDAPTRAQVRATAPVLQQHGVALTRHFYARMFQHNPELREIFNQGHQRSGTQQQALAMAVAAYAEHIDDPSVLRPVLERIAAKHVSLGIRREHYAVVGRHLLASIVEVLGEEAATPALMDAWAQAYGQLAALLAGMEQGLYDQAAAQPGGWTGWRSLRVQRRQAESEEITSFHLVPADGGPVPAFRPGQYLSVRMLVPALGYRQPRQYSLSNAPGGDHLRISVKREDGQGGEAGMVSTWLHRHLAEGDVLEVAPPAGDFVLHEDRDTPVVLLSAGVGITPMMAMLEHLARTQPARAVRFVHAARHAGVQAFGGRVRALVAGLADARAWFVHEAAPAGVPADGAAPDALGRIDPADLRERGLLPDDADIYLCGPAGFMAAQRRALLQLGVPAARLHAEAFGTGGVAEG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have oxygen carrier activity, metal ion binding, heme binding, FAD binding, nitric oxide dioxygenase activity to facilitate response to toxic substance, response to nitrosative stress.2. The protein must exhibit the following characteristics: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds; Therefore, plays a central role in the inducible response to nitrosative stress..3. The protein with the reaction NADPH + 2 nitric oxide + 2 O2 = H(+) + NADP(+) + 2 nitrate, the Charge relay system must be optimized to ensure efficient catalysis.4. The protein must bind to heme b in order to perform its enzymatic function.5. The protein should have a high affinity for NADP(+), heme b, FAD.6. The designed protein must have at least one Globin family profile, FAD-binding FR-type domain. The designed protein sequence is ",
    "response#1": "MLSQQTIDVIQSTVPVLEVHGEAITRHFYQTLFTAHPELLNIFNHANQKQGRQQAALANMVYTAALHIDNLAAILPAVRQVAHKHRSLGIVPEQYAIVGTYLLQAIGDVLGEAATDEILAAWGDAYQVIADAFIGEQEKQNAQGARKFEVIEKTSESELVTSFVLAPEDGEVVIDFESGQYLGVSVAPINHDYQEMRQYSLSDKPNGKQYRISVKREAIGVPGVVSNYLHDHIHVGDSVDIYPPAGDFHYSPEDAKTLISGGVGLTPMMSMYETIAEQQPNREITFIHSARNAKEHPFKDEVHALAENHQNLRTYVAYSEPKATDRQFDKEGYIDFAWLQSIVPDQGHYYFCGPVPFMKAVNKALKEWEVPKENIHYEFFGPAGSLED",
    "response#2": "MLSQQTIDVIQSTVPVLEVHGEAITRHFYQTLFTAHPELLNIFNHANQKQGRQQAALANMVYTAALHIDNLAAILPAVRQVAHKHRSLGIVPEQYAIVGTYLLQAIGDVLGEAATDEILAAWGDAYQVIADAFIGVESEMYAQAKQKSGWLWDGFRTFKVAKKVQESELITSFYLIPEDGKSISSYKPGQYISIKIQPAGQEFTQIRQYTLSDAPGKPYYRISVKRESGGSDRPDGVISNYLHNHITEGSLVELSAPAGDFILDANDKRPVVVLLSGGAGITPMVSMLNTLNKQRKVKESDVITSFYLVPADGQPISSYEPGQYVSIKVELPGEQFTHIRQYSLSDAPGNAYYRISVKREDAMQDKPAGKVSVYLSEEVKEGDSLQLSAPAGDSWLDDLKALPNVQTCHWFQGARKFEVIEKTSESELVTSFVLAPEDGEVVIDFESGQYLGVSVAPINHDYQEMRQYSLSDKPNGKQYRISVKREAIGVPGVVSNYLHDHIHVGDSVDIYPPAGDFHYV",
    "response#3": "MLSQETIDIIKSTVPALEQHGKAITTVFYKNMFEANPELLNIFNHANQSQGRQQTALANAVYAAAANIDNLGAIIPAVVQIAHKHRSLGIKEEHYPIVGYHLLGAIKEVLGDAATPEIIAAWGEAYGVIADAFIGREADIYQEQEKMQGARKFEVIEKTSESELVTSFVLAPEDGEVVIDFESGQYLGVSVAPINHDYQEMRQYSLSDKPNGKQYRISVKREAIGVPGVVSNYLHDHIHVGDSVDIYPPAGDFHYDVEPKTVLISGGVGITPLFSMASTIAQTQPNRDVTFLHASNSGNIHAMHGWVDELTARNDNIHYTLCYREERTEDTDREQFDKEGYIDYEWLRKVLPTKNADYYICGPMPFMKMVNEQLKEWNVPEERIHFEFFGPAENLA"
  },
  {
    "reference": "MLDLELIRRDTEKVKNALLKKMKDVDFTELIKLDDERRKLIHEVEALKNKKNEASKEISNIKSKKGKVDDSIFQNIKELSSRINEIEASLEPIKDKMDKFLEALPNIPDEDVLPGGKENNKVVHVYGEKPKFDFNPKDHVELANIHDLIDYKRGTKLSGNGFWIYKGYGAILEWALLNYFIEEHIKDGYEFILPPHILNYECGRAAGQFPKFKDEVFKVGHNEEEDNMQFILPTAETALVNLHRDEILREAELPKKYFAYTPCYRVEAGSYRASERGMIRGHQFNKIEMFQYTKPEDSDKALEELIEKAERLVKGLGLHYRLSKLAAGDCSASMAKTYDIEVWIPSMNEYKEVSSASNARDYQARRGKIRFRREEDNKVEYVNTLNASGLATSRLLPAILEQMQDKNGNIIVPEVLRKWVGKDKI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec)..2. The protein should be able to bind L-serine ligand in a variety of conditions3. The protein contains novel Aminoacyl-transfer RNA synthetases class-II family profile domains that confer a unique function or activity.4. The protein should be designed to catalyze the following reaction ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L-   seryl-tRNA(Ser).5. We require a protein that can specifically bind to the metabolites produced in the L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1, Aminoacyl-tRNA biosynthesis pathway.6. The designed protein must possess ATP binding, serine-tRNA ligase activity to support selenocysteinyl-tRNA(Sec) biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "MLDINRIVQNPEELLSTLQKRGVVSSDIEAKIKSVSEKQRILKLEVEELRAERNRVSKEIGIQKSQGKDITEISNSMKGVGDRIKSIEEELTKEEESLHELNLGLPNIADESAPKGEDDTENLEMRKWGEPRKFDFDIKAHWDLGVDLDILDFERATKISKSRFSVFKGKGARLERALMNFMVDLHTDKQGYTEMNTPVLMSPSAMMGTGQIPKFKEDMFYCEKDDMYLAPTAEVPVTNLLGGEILEQGSLPIYYTAFTQCFRREAGSAGRDTRGLIRNHQFEKVEMVKFVEPSTSYDELEKLTNNAEEILQLLEIPYRVVRLCSGDLGFSSAMTYDIEVWMPSYNRYVEISSCSNFEDFQARRANIRYRDENNKPQYVHTLNGSGLAIGRCFAAVIENYQQADGSIKIPEVLQKYLGGMTVIE",
    "response#2": "MLDLKQIRENPDYIQELLNRRSASNEYDLTPILDRDRQQRDIEATRTQLQARSNEIGKLIGQKMKSGINPQSEEIQALKTEGNDLKTQLSELEPQEKQLKSEIEALLLQLPNLPHESVPVGKDETDNIEIKKILTPRVFTFKPKEHFELAQQNGWIDFESGVKLAKSRFSVIRGFGAKIYRALIHLMLDFNEKNGFEIIYTPALVNEKMLFGTGQLPKFKEDVFKIENENLYLIPTAEVTLTNLYNDTIIGVENLPIKMTAHTPCFRSEAGSAGKDTRGMIRQHQFDKVELVAITHSKESDAMQEHMLESASEILKALELPHRFVQLCSADLGFSASNTIDIEVWLPGQNCYREISSVSNTRDFQARRAKIRFKENQKNQLAHTLNGSSLAVGRTMVALMENHQQEDGSIHIPKALEKYENLEMRKWGEPRKFDFDIKAHWDLGVDLDILDFERATKISKSRFSVFKGKGARLERALMNFMVDLHTDKQGYTEMNTPVLMSPSAMMGTGQIPKFKEDMFYCEKDDMYLAPTAEVPVTNLLGGEILEQGSLPIYYTAFTQCFRREAGSAGRDTRGLIRNHQFEKVEMVKFVEPSTSYDELEKLTNNAEEILQLLEIPYRVVRLCSGDLGFSSAMTYDIEVWMPSYNRYVEISSCSNFEDFQARRANIRYRDENNKPQYVHTLNGSGLAIGRCFAAVIENYQQADGSIKIPEVLQKYL",
    "response#3": "MIDINLIRDPKTREKVVESEKKRFRDGLAVGKAYELDRKRIEMNFRLDQINTRINQLNREIKSGYRQGKNKEDGDLSEKVAEIKGLSDEAKGLRDDVKAVEDELNKVMKGIGNIRIATPELSEDGSKLIRALANFMLDTHINNGYKEIMPAHLVNSKMLFGTGQLPKFKDDLFRVSDSDLWLIPTAEVPLTNYHYDEILNLNEPIKYVAYTKCFRSEAGSGGRDTRGIIRQHEFHKVELVKIVRQEDAMGEWEKMVNDAKNILTLLELPYREILLCTGDMGFSSAKTIDLEIWIPSEQRYRETSSISICKDFQARRAKIRYKDADGKTKYAFTMNGSGVAIDRVMAAILENYQNSDGTITVRLERALMNFMVDLHTDKQGYTEMNTPVLMSPSAMMGTGQIPKFKEDMFYCEKDDMYLAPTAEVPVTNLLGGEILEQGSLPIYYTAFTQCFRREAGSAGRDTRGLIRNHQFEKVEMVKFVEPSTSYDELEKLTNNAEEILQLLEIPYRVVRLCSGDLGFSSAMTYDIEVWMPSYNRYVEISSCSNFEDFQARRANIRYRDENNKPQYVHTLNGSGLAIGRCFAAVIENYQQADGSIKILEM"
  },
  {
    "reference": "MNDVTVVNCPTCNKQVIWDELSTWRPFCSKRCQLIDLGEWAAEEKRIPSSGDLNDSDDWSEQPLDRQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Acts by binding directly to the C- terminal domain of GyrB, which probably disrupts DNA binding by the gyrase. The designed protein sequence is ",
    "response#1": "SWGRL",
    "response#2": "MII",
    "response#3": "MSINLL"
  },
  {
    "reference": "MSSLSGKRITLIVTGSIAAYKALELIRLLTKAGASVEGVLTEGGEAFITPLSVEALTGRRAHTKMWDEHSFEMNHIELSRRADLIVIAPATAGIVAKMANGLGDDLASSVLLARNKPVLIAPSMNTAMWENAAFRRNLSQVEADGAIIVPPQTDTLACGEVGEGKMAEPSTVLQAIANHFDSAETLKGKTAIVTAGGTIERIDSVRYISNFSSGKQGNAIAISLAKAGADVTLVAGNIKDPAPQHPKLRVVSVESALEMKAAVHNSLPADIYVGCAAVCDFRVSNSSDSKIKKESGLDLTFEENPDIAKSVGTLPEGQRPSVVIGFAAETDNLIEYAQKKLASKNCDLIVANNVSNGSVFGKDSNTVHFVEKDSIQSLQELPKSEVATQLVDWIAKQFQGSD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The design of the protein must focus on the precise arrangement of amino acids in the Proton donor to enable effective catalysis of H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.2. A protein with FMN binding, metal ion binding, phosphopantothenoylcysteine decarboxylase activity, phosphopantothenate--cysteine ligase activity is needed to enhance coenzyme A biosynthetic process in a specific context.3. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..4. The presence of DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "KSILLVITGGIAAYKSLDLIRQYRKAGAAVKAIITKGGEQFVTPLSVSALCENQAYTDLWSLKDEVEMGHIRLSREADLIVVAPASADFTAKLAQGRADDLASTCLLASDKDILLAPAMNHKMWENPATQKNITTLQKRGVNIIGPENGEMACGETGLGRMSEPDTIFEELFSLCCLEGQQLLITAGPTHEAIDPVRYLSNHSSGKMGYALAQAAVLAGARVILVTGPTHLIPPAGVETYRVESAKDMYNTVMASLQEGMIFIGTAAVADYGIASVANEKIKKKGDNELTLTLAQNPDILKSVAASGKAAYVVGFAAETNDVIKHAQDKLNTKKADMIIANQVGSGLGFGSDYNQVIVVDALGQQKLPESDKLELARQLVAEIARRLNAK",
    "response#2": "MTVIVGITGGIAAYKAVGVVRDLVKRGHDVHVVPTEGALRFVGLPTLEALSRNPVTTSVWDDVAEVRHVALGRRADLVVIAPATADSLARMAHGLASDLLGTTLLATEAPVVVAPAMHPQMWEHPATRANVATLRDRGVHLVGPVVGALTGDDAGIGRMAEPEDIVAAALETARTLAAGQGAGQRVLIAAGPTQEAIDPVRFISNRSSGKMGYALAEAARDRGADVILVTGPTDLPIPPGIEPVHVCTAEDMTKAMQSRLSWSTVVIMAAAVADFRPKRPSSKKIKKSGTAWQHLELEPTDDILTLLTQQRSSQLIVGFAAETEAVAEHAADKLKRKGLDLIVGNNVAEAGSGFGSDTSAAVLISASGVESWPEMDKQAVAARLAERVAAALG",
    "response#3": "MTVIVGITGGIAAYKAVGVVRDLVKRGHDVHVVPTEGALRFVGLPTLEALSRNPVTTSVWDDVAEVRHVALGRRADLVVIAPATADSLARMAHGLASDLLGTTLLATEAPVVVAPAMHPQMWEHPATRANVATLRDRGVHLVGPVVGALTGDDAGIGRMAEPEDIVAAALALVDAHFAGPAGQRVLIAAGPTQEAIDPVRFISNRSSGKMGYALAEAARDRGADVILVTGPTDLPIPPGIEPVHVCTAEDMTKAMQSRLSWSTVVIMAAAVADFRPKRPSSKKIKKSGTAWQHLELEPTDDILTLLTQQRSSQLIVGFAAETEAVAEHAADKLKRKGLDLIVGNNVAEAGSGFGSDTSAAVLVSKTGVETWPRMPKDQVARALADRLAEALG"
  },
  {
    "reference": "MQLVFATNNLNKINEVQILMPKHIKLLSLKDIGCLEDIPETQNTIEGNAIQKAEYIKTHYGYNCFADDTGLEVEALNGAPGVYSARYAGNQRNGDDNMNKLLNELNNKENRNAQFKTVIALHLNNKIHTFTGICKGIITNTKQGQKGFGYDPIFKPQGYKETFAQMDLSLKNSIGHRGKAIKQLINFLNS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein with dITP diphosphatase activity is needed to enhance nucleotide metabolic process in a specific context. The designed protein sequence is ",
    "response#1": "PFSAVNVGFIDFELFVDLTGQEEADTDSHLVIVQNESGAFLQLNFEKSNADLLGDKLTQL",
    "response#2": "PFSAVNVGFIDFELFVDLTGQEEADTDSHLVIVQNESGAFLQLNFEKSNADLLGDKLTQL",
    "response#3": "MKPRVAIALSGGVDSSFLTFCAKRSSNKVWALFADTPLVSEEARLRVRKVAEILGVDLVSLDLDLLSLDQVKGNSPSRCYHCKRAMYELFLKWAKEEGAVVLDGTNFSDLAEDRPGLRALEELNVLSPLKVVKLTKDEIRRLSRR"
  },
  {
    "reference": "MTLTSVPAPVSLFPELELPPLPYHRPLLMIGHGTRDEDGRQTFLDFVAQYQALDHSRPVIPCFLELTEPNIQAGVQQCVDQGFEEISALPILLFAARHNKFDVTNELDRSRQAHPQINFFYGRHFGITPAILDLWKARLNQLDSPEANPQGIDRQDTVLLFVGRGSSDPDANGDVYKMARMLWEGSGYQTVETCFIGISHPRLEEGFRRARLYQPKRIIVLPYFLFMGALVKKIFTITEEQRATFPEIEIQSLSEMGIQPELLALVREREIETQLGQVAMNCEACKFRLAFKNQGHGHDHGHGHHHHGHDHGHSHGEWVDTYIEPTAYHEKIWQAP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Chelates cobalt to the cobalamin precursor as part of the anaerobic pathway to cobalamin biosynthesis..2. The protein design should take into account the physiological conditions of the adenosylcobalamin biosynthesis, Cofactor biosynthesis, cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 1/10 pathway.3. The protein must be engineered to have a cooperative binding mechanism with [4Fe-4S] cluster to ensure proper regulation.4. The designed protein should have a binding site that is specific to the target Co(2+), [4Fe-4S] cluster ligand.5. The protein should be designed to catalyze the following reaction Co-sirohydrochlorin + 2 H(+) = Co(2+) + sirohydrochlorin.6. A protein with 4 iron, 4 sulfur cluster binding, metal ion binding, sirohydrochlorin cobaltochelatase activity functions is required to enable cobalamin biosynthetic process. The designed protein sequence is ",
    "response#1": "MLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLNINNEYILYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITPRGLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITPRGLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITPRG",
    "response#2": "MLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDEGILAEKLLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITPRGLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITPRGLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITPRG",
    "response#3": "MLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDGFLKNELEVVAGIGCRRDTPFPLLATLLARQLEAQKLDPLALKAIGSVTLKKGEPGLIQLASCCRVPFKTFTAEALREFEHHFPGSGFVRKTVGVGSVSGPAAWLLSQGQLLGETLREQGVTITLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITPRGRSLIEGES"
  },
  {
    "reference": "MPIPYQRTLASNASLEGTSLHTGEKVTLTMKPAPPNHGIVFRRIDLEDMPFIPASVDNVQQVERATTLAVGSVKVHTVEHVISALAGMGIDNALIEMDANEPPIGDGSAAPYVKCIKEAGSVEQDQLANVFEIREPIHFENENGSIISIVPSKDFRVSCTHAADGGKMTQYYSASITPEVYEKEIAPARTFVFYEDVQPLLEKGLIKGGSLENAVVIRDEEILSKEPLRFDEEFARHKILDVIGDLMLSGKKFMGHVICIKPGHSPNTQVARLLKKAFSEVVSMTPSVNIPTGEGALDINEVMKILPHRYPFLLLDRIVKFEGENKCTGIKSVTINEPFFQGHFPGHPVMPGVLQLEAMAQLASIMLLRRPENQGKIGYFLSADKVKFRKPVMPGDTLFIEGEALKIKSSVAQAKCRCFVNEALVSEAELKFSLIST",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity, UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity within cytoplasm to regulate lipid A biosynthetic process.2. A key design requirement for the protein is the optimization of the active site to achieve high catalytic efficiency for a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine +   H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate.3. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.4. The protein design should take into account the physiological conditions of the Glycolipid biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MIALSNINYGIKGKKLLENISFEINSGDRLGLIGPNGSGKSTLLKIISGILKQDSGEVLLHSNISIGYLPQDPVLDENLTVLETVLLSFQDMVVLESRMEELTEKMATNDDMELLNQLQEKQDYVMMNGAYEYESKVETVLAGLGFTEYDYNRPVKSFSGGWKMRIALGKVLLQRPDILLLDEPTNHLDLEAILWLEQFLINYKGTVLIVSHDRYFLDKIVTKTFELYNGKLNTYAGNYSFYEKEKEERYEYELAAFEKAQKQIKHMQKFVERFGANASKASLAQSKIKMLDKMELVEKPIIEQPKMTFSFPQPVRSGNDVLQVENLCKGYGDKVLFENLNFSIYRGDRIGIIGPNGAGKSTLFKIIAGIEKPDSGKVIVGKNTRMAYFAQHQLETLDSSKSVLEEVTQVMEENQTREEARAVLGAFLFSGDDVFKKVKVLSGGEKARVALAKMMLEPVNLLILDEPTNHLDMYSKEVLEEALKNYEGTLIFVSHDRYFINKLATHILEVENGEIKHYSGNYSYYLEKMENRQNTEKESLGKNEKNNGSARKEKKEEKNRRRQEQRQIKEIENQINRLEEKKVELEQKMMDPALYSDSQKLFELNMELEEKQEEINALYEQWMEVSEMMEK",
    "response#2": "MNYSEALDIVKRGEYKEIPLKKINLVNRITNIKNSIDNCYNDNINWNGEKIHYMCGSTKWLSLGCSSPGYSDYNRNNIYAKNIKKLKNLFSKYGYKLSTGVSGSPILFEYIDKIIEYCKKNKINIDIDCGYRNLNLKNNFEKEIIDFEKIKKYSNSKIIIGHVSRFTYVKDLKDFKKIIDSGINFDGFTSSIGRIPLSYDEKELPKYLNKYIYSQYNETKLDFIKLIREYNVLHKLLLSTDSGNPIVPFYPSHGIIQTLATMYIYNLTSEKQLEELCFINPKKIIDKIN",
    "response#3": "MNIIDNIKYAIDNNYIKDDTLYIGSWGIGKTYLIDSFFKDEIINNKEKNKKIVYISPLLPYVEVENRVIDIILKSIGHEIAKDSKASQNIKKILGSIKITLDGIDEITGKYRKENEIESLNNLVNDIKGYGKIIVSCRTHYFDEKEINRLKQLNYICLLPLNYDVQRQIVKRYIKDDKGEEAFEKFKEYSSGNPLYLRMMADVVNDENIKQYNNIDEIINKIINTFYDDEVYNALDRNNRIVLKCIAIAEKPSKFSEIKDILDTEDIYDILNSLEEYSFIKKEKYETIFYDIFREFMYNNLSESEKKEINKKAADIVESNYANNYEEAYYHSLQAEDYNKVLDLLIKNADILYSKGQYKILKDLIDKLGNIDKDINKKKIYEIKGNIYLSMKEYEKAYYEYSKALKYCNNEEEKLKILNNIGIIYYKLGDYNKAKEYFTKCLDIAKKLGNKKSESIILNNIGVLYYEEGDYNKAKEYFTKCLDIAKELGDKKSEGNTLGNIGNVYNSLGDYNKAKEYFTKCLDIAKELGDKKSEGNTLNNIGNVYNSLGDYNKAKEYFTKCLDIAKELGDKKSEGNTLNNIGVIYYSLGDYNKAKEYFTKCLDIAKELGDKKSEGNTLNNIGVIYNSLGDYNKAKEYFTKCLDIAKEL"
  },
  {
    "reference": "MAAKSTDVRPKITLACEQCKHRNYITKKNRRNDPDRLELKKFCPNCGTHRAHRESR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "PINLDTIQQLAEKAQLTVINPEVLHQHGLVSKRDKCKILGNGLLKTKLEVTAHAFSASASAAIEKLGGKL",
    "response#2": "MPPKLP",
    "response#3": "GPINLDTIQQLAEKAQLTVINPEVLHQHGLVSKRDKCKILGNGLLKTKLEVTAHAFSASASAAIEKLGGKE"
  },
  {
    "reference": "MKLFVYHTPELTPKDQVPDCAIAVDVLRATSTIATVLSAGGEAVQVFSDLDELIAVSETWPPQKRLRAGERGGGKVAGFELGNSPLDCTPELVEGRRLFISTTNGTRALKRVQDSATVLTAAFINRAAVVQYLLEKQPETVWIVGSGWEGSYSLEDTACAGAIAHSVVEKSQLPPEKLAGNDEVISAIALYSQWQDNLLGLLHHASHGQRLLRLECHEDLKYCSQTDVLTVLPIQQEAGVFKTKN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must have high specificity towards magnesium ion binding, 2-phosphosulfolactate phosphatase activity.2. We are looking for a protein with broad substrate specificity for (2R)-O-phospho-3-sulfolactate + H2O = (2R)-3-sulfolactate +   phosphate.3. The presence of Mg(2+) is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "RFITCGSVDDGKSTLIGRLLYDSKMIFEDQMTALESDSKKVGTQGGDIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADTPGHEQYTRNMITGASTADVAVILIDARKGVLTQTRRHSYLVSLIGIRKVVLAINKMDLVDYDKAVFERILEDYRAFAARIGLTDITAIPLSALKGVNMLEEAAETPWYHGPSLMGYLETVDVEKSMLRFLTCGSVDDGKSTLIGRLLYDTKLIFEDQLEALESDSRKHGTAGENIDFALLVDGLEAEREQGITIDVAYRFFATEKRKFIVADTPGHEEYTRNMATGASTADLAVVLVDSRQGILRQTRRHSFIASLLGIRHIVLAVNKIDLIAYDQATFDDIVANYMVFAKDLGFDTIVPVPISARFGDNITTRSENMLWYKGPTLLEHLETVSIETYALSRPD",
    "response#2": "TSVTGQNQVLQYAATHMQGWRNTMEDAHISDLNIEPDVHLFAVFDGHGGSEVAIFAERHFREELMKNKNYQQKNYEKALTETFFKIDKMLQEPSGLDELNKIRGVTDEASLAGCTANVALIVGKTLYVANAGDSRSFLNRDGKPFDMSKDHKPDDEQEKKRIERAGGFVSDGRVNGNLSLSRALGDLEYKKDNRFKPEEQIITALPDVKVTQLSAADKFLLMGCDGVFETWDHQQILNFINSELKNTQNLQKAAEKLLDQLLAKDTSLGTGCDNMTCILL",
    "response#3": "RFITCGSVDDGKSTLIGRLLYDSKMIFEDQMTALESDSKKVGTQGGDIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADTPGHEQYTRNMITGASTADVAVILIDARKGVLTQTRRHSYLVSLIGIRKVVLAINKMDLVDYDKAVFERILEDYRAFAARIGLTDITAIPLSALKGVNMLEEAAETPWYHGPSLMGYLETVDVESLLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQMTALESDSKKVGTQGGDIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADTPGHEQYTRNMITGASTADVAVILIDARKGVLTQTRRHSYLVSLIGIRKVVLAINKMDLVDYDKAVFERILEDYRAFAARIGLTDITAIPLSALKGVNME"
  },
  {
    "reference": "MEGKTTMKGLAMLGIGRIGWIEKKIPECGPLDALVRPLALAPCTSDTHTVWAGAIGDRHDMILGHEAVGQIVKVGSLVKRLKVGDKVIVPAITPDWGEEESQRGYPMHSGGMLGGWKFSNFKDGVFSEVFHVNEADANLALLPRDIKPEDAVMLSDMVTTGFHGAELANIKLGDTVCVIGIGPVGLMSVAGANHLGAGRIFAVGSRKHCCDIALEYGATDIINYKNGDIVEQILKATDGKGVDKVVIAGGDVHTFAQAVKMIKPGSDIGNVNYLGEGDNIDIPRSEWGVGMGHKHIHGGLTPGGRVRMEKLASLISTGKLDTSKLITHRFEGLEKVEDALMLMKNKPADLIKPVVRIHYDDEDTLH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a specific function within the cytoplasm.2. The intended protein structure should align with strand-strand-strand-helix-strand-strand-strand-helix-helix-turn-turn-strand-helix-helix-helix criteria. The designed protein sequence is ",
    "response#1": "CEKTGLESGGVAHGGALNAAQVAHLDEDAFKGGLHRPKFDSEGLHKPHTSGGKTYETGFHYLLEAHC",
    "response#2": "PNRVLVDTGKTGSNITHTAKIISSTAGYRVPVTIERLKDGTFHVSFPAVPGKIPFVILMRALGILTDRDIVYAVSLDPEIQNELFPSLEQASSIANVDDALDFIGSRVAIGQKRENRIEKAQQIIDKYFLPHLGTSADDRRKKAYYLAYAISKVIELYLGRREPDDKDHYANKRLR",
    "response#3": "GFWYEIAFASKMGTPGLAHKEEKMGAMVVELKENLLALTTTYYSEDHCVLEKVTATEGDGPAKFQVTRLSGKKEVVVEATDYLTYAIIDITSLVAGAVHRTMKLYSRSLDDNGEALYNFRKITSDHGFSETDLYIV"
  },
  {
    "reference": "MNSLKTNLLGLNLEELEDYLEDLGDKRFHGRQLFSWIYRKGARGFEEMTDLSRGLRERLKKTATIEGIAEHKLMRSKLDGTEKFLWKLSDREMIESVLIPDEGRLTLCLSTQVGCPLGCQFCATASVGFRRNLTSGEIVSQLLKAQDRPLTNLVFMGMGEPLLNYDNLMKSIGIMVSDLGPNFGAKKITVSTVGIVPGIHRLAEEGLKLGLAISLNAPDDQLRSEIMPVNGKYPLKELLSAAKFYAERTGRRITFEYVLIGGLNDSLECAKKLSRIIQGIPCKINLIRFNPHTSQAYQAPEEEKVIAFRDYLYPRAPAVTLRESKGRDILAACGQLRAAEDDYTA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand.2. The protein's S-methylcysteine intermediate, Proton acceptor must be carefully engineered to ensure that the amino acids involved in adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine are properly positioned for optimal catalysis.3. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.4. The protein can be targeted to cytoplasm and perform tRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding, metal ion binding. The designed protein sequence is ",
    "response#1": "ISTQAGCPLKCAFCLTGIAGYKRNLKTWEILGQVAVVMEEGPREHLPWNVVVMGMGEPLLNYEATVSALRVLMDPEGFAVAPRKLTLSTVGILPALEKLMQEPVRPNLAISLHAPNQALRRVLMPIEEKYALADVMTAALRYPIPRGGLVTFEYVLLRGVNDRGHEAVSRPLLGATLDELNAWVEAQGQPKYRGKQLYQWIYQQGVRSLTDISVFPKSWRAGLADVIVGRSHLRHKSEATDGTVKYLLQLADGQIIETVGIPTQKRLTVCVSSQVGCPMACDFCATGKGGFQRNLEPHEIIDQVLTVQEDFGQRVSNIVFMGMGEPLLNTENVLAAVRSLNQDIGIGQRSITISTVGIPNRIRRLAQHHLQTTLAVSLHAPNQALRQELIPSAQNYSLRALMQECREYVEETGRRVTFEYIVLADFNDKPAHAMELANLLRGFQSHVNLIPYNPISEVDYQRPTEQRLQAFVKALEKNKVTVSIRRSRGLDKDAACGQLRAVAA",
    "response#2": "ISTQVGCKMGCKFCASTIAGFRRNLAPSEMLMQIYMAEKDSGRHVDSLVLMGIGEPLDNYDNVIKFLELLSSPKGRNMSLRHVSLSTCGVVPKIRELAKLRTGLTLSVSLHAADDKSRSEIMPVNNAWQISELLGACRDYIRDTGRRISFEYAVISGVNDSSEDARRELRGCLEDELIEICRELGIKQFRGTQLFQWVQQKAVLTWDEIRNISKSDLQTLRSRMHLYSLKHVHEQRSKDGTRKYLFRLEDGEMIECVLMDYSRKSARDRHTVCVSTQVGCAVGCTFCATGLEGFRRNLSSAEIVGQVLDITHLVRQEDPDFQVTNIVFMGMGEPLLNYDHVLKAIKLLNHEQGQAIGMRRMTISTCGVVPRIIQLAKDNPQVGLAVSLHAAQDEIRNNLIPMNRRYPLALLMEACREYSRITHRRITFEVALTADNTRREEAAALARLLKGQLGHVNLIPVNPVEGTGMERPNSEKIMEFTQVLEDAGIAVSSREERGADINAACGQLRETH",
    "response#3": "VGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGSGEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLHSPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKESNAVIAYANNKLTVCCSSQAGCAMACVFCATGKQGLIRNLFLGEIVDQINVVADDFQQRVSNIVVMGQGEPFANYHAVIEALHILNSSRYNDIGARHITISTCGIVPNIKKLAHEGMQYTLAVSLHSAIQQTRNVIMPRVIQFPLTDLKQALEYYVQITNRRVSLEYALIKGINDSQEHLQALCVFCNNLLCHVNIIMLNKIENSPFAPVSEKTRRLWLNELEKHGIPATARISRGADTSGASYVVQRPLLGATLDELNAWVEAQGQPKYRGKQLYQWIYQQGVRSLTDISVFPKSWRAGLADVIVGRSHLRHKSEATDGTVKYLLQLADGQIIETVGIPTQKRLTVCVSSQVGCPMACDFCATGKGGFQRNLEPHEIIDQVLTVQEDFGQRVSNIVFMGMGEPLLNTENVLAAVRSLNQDIGIGQRSITISTVGIPNRIRRLAQHHLQTTLAVSLHAPNQALRQELIPSAQNYSLRALMQECREYVEETGRRVTFEYIVLADFNDKPAHAMELANLLRGFQSHVNLIPYNPISEVDYQRPTEQRLQAFVKALEKNKVTVSIRRSRGLDKDAACGQLRAL"
  },
  {
    "reference": "MQCPSCQNTDSRVLESRSADAGKCVRRRRECLNCDFRFTTYERVETIPVTVIKRSNAKEIFCRSKLLNGLTRACEKTSINNQKIEAIVDEIETHLQQSNLKEISSVDLGEMILLHLKSVNEVAYIRFASVYRQFNGIEDFIDTLESFKPSNKFAAIS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have DNA binding, zinc ion binding to facilitate negative regulation of DNA-templated transcription.2. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.3. The presence of ATP-cone domains is critical for the interaction of the designed protein with its intended targets.4. The protein must exhibit the following characteristics: Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR- boxes.. The designed protein sequence is ",
    "response#1": "MVTKRSGATEPFSRSKIIAGVRRACQGRPVTEDALALLGQRVEEEIRSRGVAEVSSHEIGLTILGPLRDLDEVAYLRFASVYRSFS",
    "response#2": "MVTKRSGATEPFSRSKIIAGVRRACQGRPVTEDALALLGQRVEEEIRSRGVAEVSSHEIGLTILGPLRDLDEVAYLRFASVYRSFR",
    "response#3": "MVTKRSGATEPFSRSKIIAGVRRACQGRPVTEDALALLGQRVEEEIRSRGVAEVSSHEIGLTILGPLRDLDEVAYLRFASVYRSFK"
  },
  {
    "reference": "MNLLEMSYEEVVDVVKQMGLEKYRADQLLDWVYKKRVFTFEGMTNLSKEHRALFREKFTVKLPRLIEKRVSKIDKTTKFLYELEDGNTIESVLLFHEGYATACISTQVGCPIKCAFCATGQSGYVRNLTVAEIVGQVLAVENDSGRNIRNVVYMGMGEPFLNYDNVVKSIKILIHPKMKDMGVRRITVSTVGIPDKITRFADEGLDVRLAISLHAASNEKRDTIVPINRKYSIEEVLQAAKIYQEKTDRRITIEYILIKEFNDFDEDARRLAKLLKDLKAYVNLIPVNATSASFERPSHWKMVRFKEILLENGIEAELRQEKGADIEAACGQLRLRRMQAKS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have tRNA binding, metal ion binding, tRNA (adenine-C2-)-methyltransferase activity, rRNA (adenine-C2-)-methyltransferase activity within cytoplasm to regulate rRNA base methylation.2. The protein should be able to bind S-adenosyl-L-methionine, [4Fe-4S] cluster ligand in a variety of conditions3. The designed protein must have a Proton acceptor, S-methylcysteine intermediate that is structurally stable and resistant to denaturation.4. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.5. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MTIEELSRFLAEMGEPKFRAKQVFSWLHQKRVLSFDEMSNLPAALREKLKESSIVQALEYLDRQTICVSTMVGCPARCAFCATGAMGFGRNLTPGEIVGQVLAVAGGEGVAPREIRNLVFMGMGEAMLNYEHTMAAARLLLHPQALGMSRRRVTLSTVGIAKGIRRLAEEDDLGIKLAISLHAPDEATRQKIIPTGAANSIAEIMSAARDYQAVTGRRVTLEYTMLRGLNDHLWQAELLADLLRGLVSHVNLIPMNPWEGSNFESSSEAQIQAFYDALQARGVDVSVRRSRGRDRLAACGQLAARR",
    "response#2": "DGNRRTVCVSSQVGCAVGCAFCLTARMGFRRNLTPGEILGQFFVLEERTDIRKVGYNVVFMGMGEPMANRANLEEALAILEDEEGMGISWRRITVSTSGLADQLEAFAKSAVCPRLSLSLNAPTDELRSRLMPVNDKYGLRRLRSVLQNLSRRGRERISLEYILLGGVNDGTEEAEAVAGFARGLKVKVNLIQFNEAKDLPFVPSDEERSRSFQAVLLRHGVPTSIRKSRGQDIDGACGQLAAQG",
    "response#3": "STQVGCQMGCRFCLTGKGGFTRNLLPSEIVDQITMARFNTPEGDNIKNIVMMGMGEPLANYKNTVQAIKIITNDYGPAVSPRKVTVSTCGIVPMIEHLGNDTPVNLAVSLNASDNETRNMLMPINRIYPIESLIEACRTFPMPRRRRITFEYILIAGVNDSGEITYEQRRTLCISTQAGCAMGCVFCATGQMGFKRHLSAGEIVEQVLYYARMLKLEDEKVTNIVLMGMGEPFHNYDATLKAIDLLNDANGFGLGARRFTISTVGLVPMIRKFADEERQINLAISLHATTNEERTSLLPINKKYSIEQVLEACRYYVTKTNRRITFEWALIAGKNDSEATAKHLAHLLHGMLCHVNVIPLNPTSQSIYQPSSKEQTRLFQQTLEKAGIPCTIRLRRGIEAN"
  },
  {
    "reference": "MSANQSPAGQPPTATYDSAFLKACRREPVPHTPVWFMRQAGRSLPEYLKVREGIPMLESCMRPELVAEITLQPVRRHGVDAAVYFSDIVVPLKAIGIDLDIKPGVGPVIAEPIRTRADLARLRDLTPEDVAYVTEAFGLLTRELGATPLIGFAGAPFTLASYLVEGGPSRNHEHTKALMYGDPQLWADLLDRLAGITAAFLKVQIEAGASAVQLFDSWVGALSPADYRRSVLPASRKVFEAVSGYGVPRIHFGVGTGELLGLLGEAGADVVGVDWRVPLDEAARRVGPGKALQGNLDPAVLFAGREAVETKTREVLDAATGLEGHVFNLGHGVLPTTDPDALTRLVEYVHTQTTR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have a high affinity for substrate.2. The designed protein must possess uroporphyrinogen decarboxylase activity to support protoporphyrinogen IX biosynthetic process under certain conditions.3. The protein should be optimized for activity in Porphyrin-containing compound metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "ESPYLAAVNGRKPHRVPVWFMRQAGRSLPEYRALRAQHDMLSACFDAELVCEITLQPVRRHDVDAAILFSDIVVPLLGAGIDLDIVAGVGPVIASPVRTAADVAAMKPLERQRVQPIADAVRLLVSALVEVPLIGFAGAPFTLASYLIEGGPSRHHARTKAMMLADPDSWHALMEKLTDITVGFLRVQLDAGVDAVQVFDSWAGALSLANYRSYVQPHSARVFAALADYGVPMTHFGVGTAELLGAMSAALKPAPATVVGVDWRTALADAATRVMPGTALQGNLDPVVLLAGWPVVETAARAVVDDGRRAVDAGAAGHVFNLGHGVLPETDPGVLTELVSLVHRG",
    "response#2": "ESPYLAAVNGRKPHRVPVWFMRQAGRSLPEYRALRAQHDMLSACFDAELVCEITLQPVRRHDVDAAILFSDIVVPLLGAGIDLDIVAGVGPVIASPVRTAADVAAMKPLERQRVQPIADAVRLLVSALVEVPLIGFAGAPFTLASYLIEGGPSRHHARTKAMMLADPDSWHALMEKLTDITVGFLRVQLDAGVDAVQVFDSWAGALSLANYRSYVQPHSARVFAALADYGVPMTHFGVGTAELLGAMSAALKPAPATVVGVDWRTALADAATRVMPGTALQGNLDPVVLLAGWPVVETAARAVVDDGRRAVDAGAAGHVFNLGHGVLPETDPGVLTELVSLVHPR",
    "response#3": "KNDRYLKALLREPVDMTPVWMMRQAGRYLPEYKATRAEAGDFMALCRNADLACEVTLQPLRRYALDAAILFSDILTVPDAMGLGLSFGAGEGPKFARPIENKAQVDNLPIPDPESELQYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGSSKAFTKIKKMMYADPKILHALLDKLADSVILYLNAQIKAGAQAVMVFDTWGGVLAHNEYKEFSLRYMHKIVDGLIRENEGRKVPVTLFTKGGGLWLEAIAETGCDAVGLDWTVDIADARRRVGHKVALQGNMDPSVLYAQPERIELEVKQILAGFGQGSGHVFNLGHGIHQDVPEQSPKVFVDAVHEIV"
  },
  {
    "reference": "MSQEKVQRHGGGDGEEESGPEAAGQERREKLGEDVDAILDEIDDVLEENAEDFVRAYVQKGGQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in protein pupylation, proteasomal protein catabolic process, potentially with improved properties such as stability, specificity or selectivity.2. The protein must exhibit the following characteristics: The tagging system is termed pupylation.. The designed protein sequence is ",
    "response#1": "MQ",
    "response#2": "MPGFLRKITG",
    "response#3": "MPTQSSST"
  },
  {
    "reference": "AARSVHAFSARLLGSGELLSLASLRGKVLLIENVASLGTTIRDYTQMNELQKRHGGRGFMVLGFPCNQFGHQENAKNEEILNSLKFVRPGSGFEPNFMLFEKCEVNGEKAHPLFTFLREALPAPSDDTNSLMTDPKLIIWSPVCRNDISWNFEKFLVGPDGVPVKRYSRRFETINIEKDIEDLLTKMPKEV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytosol that is critical for negative regulation of inflammatory response to antigenic stimulus. The designed protein sequence is ",
    "response#1": "MVFLQGVAGIGKSLMLTKLMLAWSEGMVFQNKFSYIFYFCCQDVKKMKRASLAELISKEWPNASAPIEEILSQPEKLLFVIDNLEVMECDMSEWESELCDDCMEKQPVNILMSSLLRRKMLPESSFLVSATPETFEKIEDRIECTNVKMMAGFNESSIKVYFYSLFQR",
    "response#2": "MVFLQGVAGIGKSLMLTKLMLAWSEGMVFQNKFSYIFYFCCQDVKKMKRASLAELISKEWPNASAPIEEILSQPEKLLFVIDNLEVMECDMSEWESELCDDCMEKQPVNILMSSLLRRKMLPESSFLVSATPETFEKIEDRIECTNVKMMAGFNESSIKVYFYSLFQR",
    "response#3": "MVFLQGVAGIGKSLMLTKLMLAWSEGMVFQNKFSYIFYFCCQDVKKMKRASLAELISKEWPNASAPIEEILSQPEKLLFVIDNLEVMECDMSEWESELCDDCMEKQPVNILMSSLLRRKMLPESSFLVSATPETFEKIEDRIECTNVKMMAGFNESSIKVYFYSLFQS"
  },
  {
    "reference": "MKLYDNGVAVLGCGNLGNAIAKGLVASKQFKSNQIVLTKRNLSTIEPLKREGYHVTTSNHDAVSRCKIVIVCVVPAQLDDLLDSIKQSVTENHIIISVVSGASIEDIRSHLEKDVPIVRAMPNTAIQHCQSMTCLAIRSSHQKSTNSPSDKAKDNALEVAKKIFNCLGMSIVLSEEQIVPATALCACGIAFFCRAIRAAAQGGCEIGFHAEDAIRIAAQTAKGAATLLLENNFHPEYEIDKVTTPQGCTIAGLNQMEHAGFSSAMIKGIVTSSDKAASLYTQKQQNKKQQQLKQQQHQQHQHQQHQQHQQQVQQQEPHQYQQQQQQSHQQSQYNQGHNYGHQNQHHHNHDDQHQNYYNQDQKRRNNRKHRSNENYDNHHHHNQQYQQHQQPTQQESQEQTQQPEQTQSTNQSNQRRNSESRNGKSPQKQPQKQSQVQQPSSTTENTDQQQQQQPPQEQQQQQEQPQQPQEQQQQPNVVDEAERPKEQQQQPQQQQQTIDKKGYNNNRRGGRHYSYNNNYNSHHHRHNGINKNSSSMYHDEKRHEVKTEQIN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The desired protein should be able to perform L-proline + NAD(+) = 1-pyrroline-5-carboxylate + 2 H(+) +   NADH The designed protein sequence is ",
    "response#1": "TMDASTALCGSGPAFFALILEAAIDGAVAMGLPRAEAQRMAAQTMKGAAGLVLSGEHPALLKDKVTTPGGCTIGGLMVLEEGGVRGTVARAVREATVVASL",
    "response#2": "TMDASTALCGSGPAFFALILEAAIDGAVAMGLPRAEAQRMAAQTMKGAAGLVLSGEHPALLKDKVTTPGGCTIGGLMVLEEGGVRGTVARAVREATVVASL",
    "response#3": "EEAMVSAPALCSCGTAFFCRIIRAAASAGCEIGFHAEDAVRIAAQTAKGAAIMLLKNDSHPESEIDRITTPSGATIAGLNTMEHNGLSSAIIKGIVMSAEKSR"
  },
  {
    "reference": "MKIAFQNGENLLHVSDNSSECDKDTFFLLTDQNARYQGNAQEQGCTHFISPTECLSLLGIDKKIKIIGITGTNGKTTTAAAIYSLLLDLGQKVGLQGTRGCFINEKRVEDKSLTTPPILQTIKNLKMAVDEGCEYFVMEVSSHAIVQNRIDGLFFALKILTNVTQDHLDFHKTIDEYIAVKSRFFEDESLKLINSDERHIRFNRINAFTYGIEHPATYKVLAYSLKEGISAAIAKKEKVYDFESSLHGFFNLYNLLAAISAVDLLHVAGMEEICEAVSHFGGVEGRMQVVSQDPLVIVDFAHTPDGMEKVLESMKDKNCIVVFGAGGDRDRTKRPKMGAVAERYAKWMIVTSDNPRSENPDAIIEEIVAGIQRTESLHVKVDRYQAIEEALGMQKKSDVVLILGKGDETYQEVAGVKYPFDDRNVVLEILARLAKQK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. Ideal protein should have a binding affinity for key enzymes in the Cell wall biogenesis, peptidoglycan biosynthesis pathway to enhance the overall efficiency of the pathway.2. The designed protein should be involved in cell cycle, cell wall organization by localizing to cytoplasm and exhibiting UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, ATP binding.3. The designed protein should be able to compete with endogenous proteins or inhibitors for meso-2,6-diaminoheptanedioate binding.4. The protein contains novel Mur ligase C-terminal domains that confer a unique function or activity.5. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "MTGRMEFVGRHACGAAVFVDYAHTPDALETALKSLRQSEQFERIGVVFGCGGDRDRGKRPMMGEVAAAHADFSIVTDDNPRSEDPQTIRDAILATHPRAIECGDRKEAICLGIKMLSNQDVLLVAGKLGG",
    "response#2": "VTGTNGKTSTVDLLRQIWSGAGINAASLGTLGLVRGGMEPQPGMTTPDQIALHKMLHTLSQEGCQHLAMEVSSHGLHQHRVDGVTFQSGAFTNLSRDHLDYHVSMEAYLEAKLELVRRCIRKNGLLASAATGEAAIAIHAACEKSGVRLWDYHKQDLNFFGFKVMSLSAEGAKVHITSQGHLQCVTVPFIARFQLENLALAAVLCNALGISDEEAIRALGKFEGAKGRMQRVGGGAKPLVVVDYAHTPDALEKVLRSLRELMLHRSPVTGHPSRLICVFGCGGDRDRGKRPQMGRIAARLADSVVVTSDNPRSEDPHAIIVDILEGARRGKRELCVNADRRDAIEAAIAGAQRGDIVLVAGKGHEDYQEIAGVKHHFSDQEVVQELL",
    "response#3": "MKLITGTKGKTTIVYMLRYIFEAARVRYGIIGTISNIVNHVVRKTGRTTPESLQLQKTFYEMADEDTQYVAMEVSSQGLMLHRVYGTLFDIGVYNNISRAHIGPKEHESFEDYLANKIKLFSICKEGLINVDDAHAQDVISGATCKIYTYGINHKADFFADGITKFYDKVEFDFNTPTYKKRMTVNMPGKYNVYNGLAAAATAFALGVSLDVIKKGLEAVKGVAGRFDYIVSPVDRIMGIVDYAHTPDALLNVLSTLQKLRKGGEKIITVVGAGGDRDKTKRPVMAEVACEHSDRVILTADNPRSEDPMDILRDMESGLGSAAKRKYLVVPDRTEAIKTAITLAGKGDIILVAGKGHEDYQEIKGEKYHFDDREELRRALEER"
  },
  {
    "reference": "MATINQLVRKPRVKLVDKNNVPALQECPQKRGVCTRVYTTTPKKPNSAMRKVARTRLTNKYEVTAYIGGEGHNLQEHSLVLIRGGRVKDLPGVRYHIVRGALDTAGVKDRKKGRSKYGAKRPKA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be involved in translation by localizing to small ribosomal subunit and exhibiting tRNA binding, structural constituent of ribosome.2. The protein must exhibit the following characteristics: Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. The designed protein sequence is ",
    "response#1": "VKLRSCCDPLFEAPEVGRLVNLVMVSGKKTVAARIVYTALAMLSPDRRRSYELLMQALHNIKPSAEIRTRRMGGASYRVPAEICSKRRMSLALRWLKSAAMRRRERYAHVRLYNEILDALSRRGGAVRQRDEVHRLAHANR",
    "response#2": "VKLRSCCDPLFEAPEVGRLVNLVMVSGKKTVAARIVYTALAMLSPDRRRSYELLMQALHNIKPSAEIRTRRMGGASYRVPAEICSKRRMSLALRWLKSAAMRRRERYAHVRLYNEILDALSRRGGAVRQRDEVHRLAHANW",
    "response#3": "VKLRSCCDPLFEAPEVGRLVNLVMVSGKKTVAARIVYTALAMLSPDRRRSYELLMQALHNIKPSAEIRTRRMGGASYRVPAEICSKRRMSLALRWLKSAAMRRRERYAHVRLYNEILDALSRRGGAVRQRDEVHRLAHANA"
  },
  {
    "reference": "MSWLTEYVRPKIRTLLGRRDVPDNLWVQCPACQQMIFARDLEKNQRVCTHCDHHMRGTALERLKWTLDEGYTRIELPKAPQDPLRFRDSKRYTDRLRDAREKTHLDDAIVVAHGTIEGQKAVVAAMAFEFMGGSMGAAVGEGIVAAAQLAVLQKAPFIIFTASGGARMQEAAISLMQMPRTTIATRMVKEAGLPFIVVLTDPTTGGVTASFAMLGDIQIAEPKALIGFAGARVIEDTTREKLPEGFQRAEFLLEHGIVDMVVRRADMRATLSRVIALLTEGVTLPKVESVASLTEAKQPARTADA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be involved in fatty acid biosynthetic process, malonyl-CoA biosynthetic process by localizing to acetyl-CoA carboxylase complex and exhibiting zinc ion binding, ATP binding, acetyl-CoA carboxylase activity, carboxyl- or carbamoyltransferase activity.2. The Zn(2+) binding site should be stable and able to withstand changes in environmental conditions.3. A protein that is capable of catalyzing reactions within the malonyl-CoA from acetyl-CoA: step 1/1, Lipid metabolism pathway with high efficiency and specificity.4. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.5. The protein should have a fast turnover rate for acetyl-CoA + N(6)-carboxybiotinyl-L-lysyl-[protein] = malonyl-   CoA + N(6)-biotinyl-L-lysyl-[protein]. The designed protein sequence is ",
    "response#1": "MWSRCAGCSSLLYRKRLRRNLDVCPECGAHARLGAPERLRQLVDPGSLCLLPDLLPATDPIDFVDVLPYPHRLTAARAGTGLPEAVVCGTATIGGHPAALAVMDFRFLGGSLGSAVGELITRTAERALDDRIPLVLVTASGGARMQEGALSLMQMATVSQAIAGLREAGLLTVSVLTDPTYGGVAASFATNTDLVLAESGARMGFAGPRVIRQVTGRELPEGFQTADFLLRHGQVDMVVQRRSLRGRLSALLAAADHCRPSRPPVPRQEPVVRDPEALTVRAPEEMLRLARRLDRPTTLDHILQWMDGFVELHGDRGQADCPAIVAGAGLLDGLPVMVIGHQKGHTTAQLVRRDFGMPSPAGYRKAARVLRLAAKLRIPVLTLVDTPGAAPGIEAENRGQAFAIAECIRTMGKLPVPVVSVVMGEGGSGGALALAAADRVLICANAYYSVISPEGCAAILWKSPEARDTAAAALKLDARSLLRLGVVDGVVPEPDGGAHTDPAGVSELIRSAVVSALRPRDLPAGETWEAVRLARALDRPTARDYIQWIADDFVELHGDRLGGDCRAITGGLARLDGAPIMIIGHQKGHTAKELARAGFGMPTPAGYRKAARLMRLAAKLGVPVVTLVDTPGAHPGIEAEAEGQAAAIADSLRLMTGLPVPVVSVVTGEGGSGGALALAVANKVLAFSNAIYSVISPEGCAAILWRDSGAAPQAARELRVDARSLLRLGIVDAVVPEPGGGAQGDPGAAAARLKAAITAGLDACLAV",
    "response#2": "TVHKPSDLADVDPWQVVRDARDPRRPTSADYVTELVQGFEELRGDRVSGDCSAIIGGFGLLQGMPVLVLGHQKGHETDELVQRNFGMASPQGYRKTTRLMRLAAKLKVPVLTFIDTPGAYPGITAEEDGQSFAIAENLRVMSSLPVPIVSVITGEGGSGGALALGVADRVLALSGSCYSVISPEGCAAILWKSPQEAPLAARALRIRARDLLGSEVVDAVVPEPPSGAAEDGAAMARALEGAVVEAFLDSAEVRERVAWDAVQQARGITRPLALDYANLLLDGFVELHGSRLAGESGALIGGVGLLGGEPVILLGHQRGSNAPELVRRNFGMPQPDDYRKVQRLARLAAKLRIPVVTLIDTPGAYPGVSAEERGQAFAIAETLRVFAALPTPVVAVVIGEGGSGGALALGLADKVLICENAVYSVISPEGCAAILWKSAAAADQAAEQLRLTAGDALSLGVVDGIVPEPRGGSEQDPGRAADFLANALSRSLRPRDLPAGETWEAVRLARALDRPTARDYIQWIADDFVELHGDRLGGDCRAITGGLARLDGAPIMIIGHQKGHTAKELARAGFGMPTPAGYRKAARLMRLAAKLGVPVVTLVDTPGAHPGIEAEAEGQAAAIADSLRLMTGLPVPVVSVVTGEGGSGGALALAVANKVLAFSNAIYSVISPEGCAAILWRDSGAAPQAARELRVDARSLLRLGIVDAVVPEPGGGAQGDPGAAAARLKAAITAGLDRLSADDLRKPKTPPDAARPELPAVMVDDPFVLPEQGAWDVVQRARNPQRPSALDYMRLALDGFDELHGDRLGHDCPAVVGGIGRLNGFPLMVIGLQKGHDAAELAQHNFGMATPAGYRKANRLMRTAERLGLPILTLVDTPGAFPGRSAEETGQAAAIAENLRLLTGISVPVVCVITGEGGSGGALALAVADRVYILENAVYSVISPEGCASILWHDSGAAPQAASSLRMDPRSLLELGIVDAVIREPEAGAAADHVTAADRLRQAVTAALR",
    "response#3": "MWSRCAGCSSLLYRKRLRRNLDVCPECGAHARLGAPERLRQLVDPGSLCLLPDLLPATDPIDFVDVLPYPHRLTAARAGTGLPEAVVCGTATIGGHPAALAVMDFRFLGGSLGSAVGELITRTAERALDDRIPLVLVTASGGARMQEGALSLMQMATVSQAIAGLREAGLLTVSVLTDPTYGGVAASFATNTDLVLAESGARMGFAGPRVIRQVTGRELPEGFQTADFLLRHGQVDMVVQRRSLRGRLSALLAAADHCRPSRPPVPRQEKPKTPPDAARPELPAVMVDDPFVLPEQGAWDVVQRARNPQRPSALDYMRLALDGFDELHGDRLGHDCPAVVGGIGRLNGFPLMVIGLQKGHDAAELAQHNFGMATPAGYRKANRLMRTAERLGLPILTLVDTPGAFPGRSAEETGQAAAIAENLRLLTGISVPVVCVITGEGGSGGALALAVADRVYILENAVYSVISPEGCASILWHDSGAAPQAASSLRMDPRSLLELGIVDAVIREPEAGAAADHVTAADRLRQAVTAALRRPERSSAIVTDPDQLPHAYPWDQVRKARSLDRPTALDYLGYAFEGFRELHGDRISGECPALIGGTARLDGLPVMVLATQKGHTPTELAARNFGMASPAGYRKAARLMRMADKLGVPIVTLIDTPGAYPGADAEERGQAVAIAENLRLMATLSVPVVAVIIGEGGSGGALALAVANRVLMFSDAVYSVISPEGCAAIVWKDDTAVTAAAQALRLNARELLRLGVVDGVLPEPDGGVGADPVQAAETLRSALSSALH"
  },
  {
    "reference": "MELKQFDGQKKSELSLIEVAHAILSQHGDVMAFADLTNAVQSYLGKSDEEIRERLSQFYTDLNIDGSFISLGDNMWGLRAWYPFESIDEAVIHTDDDEDEDRPKRKKVNAFLADAGDDDDVIDYDDDDPEDDDNYDDDDDQDDDTDDSASSKYDELAGVDDTDDDVADETLPDGIEGQLSELNDDDDDDDYDDEDDESK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be able to regulate DNA-templated transcription. The designed protein sequence is ",
    "response#1": "PVARDHTHGRAAKFRTSANIWMNEAMRAAGYEPYNVSMSNHDIERGNRYFYFAKDLTIPYRNDPVSDNTGFVFCDVDYYADMEKWMQHFKPMLLYTLVPESLSYHCDDHSFHVNDDRVFFDVRGGASYSHQLWDYTGDTICVV",
    "response#2": "PVARDHTHGRAAKFRTSANIWMNEAMRAAGYEPYNVSMSNHDIERGNRYFYFAKDLTIPYRNDPVSDNTGFVFCDVDYYADMEKWMQHFKPMLLYTLVPESLSYHCDDHSFHVNDDRVFFDVRGGASYSHQLWDYTGDTICVV",
    "response#3": "RRTLARIAHEILERNQSSRDLVIIGMYTRGVPLANRLAENILRFEGLEIPVGTLDFSLYRDDLDSQRFHPTIKNTDIPFSINNKIVVLVDDVLFTGRSTRAAMDALIDYGRPKAIQLAVLVDRGHRELPVRADYIGKNIL"
  },
  {
    "reference": "MVKAKKFILAKYFVNAPKTTDFKLVEEELPPLQDGEYLVQAEYIAIDPYVRVFSPKCPLESTIISSQVAKIVESKNPEYPVGKRIVGYLGWRTHTVVKPETSQNLAIHDTYILPDIGDLPPSLTLGMLGTSGNTAYFGLTEICKAKPGETIVISGAAGGVGSHVGQIAKIMGLTVIGICGSDEKCKWLTDKLGFDFAINYKTMPIAESLRKAAPQGVDCYFDNVGGDISNAVLYQMRLFGRVAVCGSISSYDADITSLPQCTILQPALLFNQLKMEGFIVNRWKDRWLEGIQQNLQWIQEGKLTYRETITKGFENMFDAFIGMMRGQNIGKAIVQV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECR",
    "response#2": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECR",
    "response#3": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECR"
  },
  {
    "reference": "MVVERLQTNLGQQLELLESLKTVVAQEQQLLCSGRIQGIVLQGVTEQKSSILATLAYLDQTRLTTEKNINIQAPYSNVPALAERWQRILELAEGLRYSNLHNGLLLQQHIEHNTQALAVLNTRHGQSLYGPDGHAKGASLLGRKIGI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Required for the efficient initiation of filament assembly.2. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in bacterial-type flagellum assembly. The designed protein sequence is ",
    "response#1": "GKFTHATHATSIDVIDSLGTKHAMRIEFYRSGGAEWNFRVIVPEPGELVGGSAARPNVFEGGRLHFNNDGSLAGMNPPLLQFDPKNGADAPQRINLAFGSSGSFDGLTSVDKISETYAIEQNGC",
    "response#2": "EKFTHATHATSIDVIDSLGTKHAMRIEFYRSGGAEWNFRVIVPEPGELVGGSAARPNVFEGGRLHFNNDGSLAGMNPPLLQFDPKNGADAPQRINLAFGSSGSFDGLTSVDKISETYAIEQNGC",
    "response#3": "LKFTHATHATSIDVIDSLGTKHAMRIEFYRSGGAEWNFRVIVPEPGELVGGSAARPNVFEGGRLHFNNDGSLAGMNPPLLQFDPKNGADAPQRINLAFGSSGSFDGLTSVDKISETYAIEQNGL"
  },
  {
    "reference": "MPKTPNGKLDRKSIAALLLRNAKRDMNGVVNDVEKMTVREGELRLLWERVLPTLGDLRLGPSSDFFMCGGNSMLLMKLQKAIKETTGIRVSTKDLYESSTLRAMTHCVFDRANRADDDAAPIDWAVETSLPASLQTQIQDLATSSPPEAGGHGTNGTEVLLTGATSFLGSHLLRSLLSSPRVKKVHCVAVPADEQATLFSHDTRIVCYSGTLLSPTLGVTPQERRTLEQSVHVIVHAGAHGHCLNRFDSLRAPNLQSLHFLATLALPRCVTILFLSSSRVVLLSGDTAPAPASMRSYPPAVDGKDGYTASKWAGEVFLENLVAHVENVASSASSPSVFWRSSLNVEVHRACTLVSESAPNSDAMNAILRHSLDMRCAPRLERAEGYLDFAPMESIVAKITVHAVEMATAVQQQQQQQQRQSQPPRDDAADGSPTERARGLRIAITLAVSSRPWATLGRIWRGRMVGDPKNWIYKSGL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that The non-reducing polyketide synthase Dhc5 then conducts four additional chain extension cycles, producing the unreduced part of the nascent octaketide from C-1 to C-8 in 10,11- dehydrocurvularin The designed protein sequence is ",
    "response#1": "EESCTQIGSISAFYDALERSGFDLGPTLKNLVDVRTSATSPHCMTKMAAPSIAEHMPKGYNFPYIIHPSTMESMAHAILHVCTTKEAPVGSALLARYIDNIWISNKIPMDPGHLFVTVADGEQVSPGKWRCAITVWNDATKEVVIQIRGTELCLLSAE",
    "response#2": "STCAQYLVSKSSSPKVQVVFRASISDPGFMELIDGHKMQGIGLCSGSVFCEAAFAAAKYALEYSGRRNVTQPWLTLQKPELLLPLTKKLAGADGSLITTAVMDSPSAHRISVTFKLTSGSESHELGSCIVNFRDPAKTQADWDRVSYFIQARMDEVIKNAKEGPGHRMQPEVFYALFANAVEFSTDFQGVDEAYIAKDFQEAAALVTLPHDPAGTKFTFSPYWGEALVHLAGFMVNGNPSKSPQKTFIVMGFESVEQTVPLVPGKKYMAYTRISKWVKETAYCDAVVFDPETSNIILQCIDLRYQELPRVTWKHVLQ",
    "response#3": "TSSVQQIIFEEYDESMGRMEALSDLHHPDLQGAADGHKIHGRSVVTGSIWADITLTVGEYLYKQMVPGGKMPHMNVKGMEVLEAQVLHPDMSQFIQIEGVLDLPRQQTAVRLYAASANGTRNTDKPFASATVCYEEAQDWQDQWQMTSHLVAARANSFWEMAAGGSDDNARPAGKGGPRVNNFFRSIAYQLFANVVDYGARYRGMQRVALSEDTLEATADIVLDKDRHGTWHTPPHWIDSAFQLAGFVMNSFGVQGDGKISGSSRDFFYITPGWRHFRLLERLEPGPEATYRSFVRMFPVGSEPGAYSGDIYLHRGKRLVGVCAGIKFKAVPRALMPVLFR"
  },
  {
    "reference": "MKEKIHPEYKEAKVVCACGETFVTRSTKPVIKVDICSKCHPFYTGKQKIVDTEGRVEKFMKKYSKK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be involved in translation by localizing to ribosome, ribonucleoprotein complex and exhibiting metal ion binding, structural constituent of ribosome, rRNA binding. The designed protein sequence is ",
    "response#1": "MNKIKKGDKVVVLAGKDKGKRGEVLKAIPN",
    "response#2": "SPIGAKVTLRGERMYDFLDKLVNVSLPRVRDFHGVSAKSFDGRGNYTLGVREQLIFPEIDYDKVDRVRGLDVVIVTTSNTDEEARELLTQFGMPL",
    "response#3": "GGDKVVVLAGKDKGKRGEL"
  },
  {
    "reference": "MPLLDSFTVDHTRMQAPAVRVAKQMNTPKGDAITVFDLRFCVPNKAILSERGIHTLEHLFAGFMRNHLNSESVEVIDISPMGCRTGFYMSLIGTPKEQEVANAWLHAMEDVLQVTKQEDIPELNEFQCGTFEMHSLPQAKAIAQDIISAGIGVNQNAELTLDESLLSKG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Fe cation binding site should be stable and able to withstand changes in environmental conditions.2. For general function, the protein need meet that The regulation of gene expression in response to changes in cell density is called quorum sensing3. The designed protein must be able to perform iron ion binding under specific conditions. The designed protein sequence is ",
    "response#1": "WDTSFQVFYFKLDDQHRAIFETLFNSTNDNTPGNLQLFYIVTANHFEEEEGWMVSASYGGYDAHKKLHEEFLAKVRSFSAPVSKENLFYAKDWLVQHIKTIDFKYKQQ",
    "response#2": "MLLTLPNLLSPADLLAARALLSAAPWADGRATAGIQAAQVKNNEQLPSDCEATRAIQAMVMKGLDGSPVFFSAALPKKMFPPRINRYGGDSNFYGNHVDNAIRAVPNSPQRVRTDISCTVFLNDPGDYDGGELTIADTYGEQRVKLPAGHAVLYPGTSLHQVRPVTRGHRLACFFWIG",
    "response#3": "WDTSFQVFYFKLDDQHRAIFETLFNSTNDNTPGNLQLFYIVTANHFEEEEGWMVSASYGGYDAHKKLHEEFLAKVRSFSAPVSKENLFYAKDWLVQHIKTIDFKYKQH"
  },
  {
    "reference": "MIDYKINEILVKQLNDDYNCSICVDPVLNSLPLEQHQALSCKNGHLLCQACWGKQLALRKECCICKIKVESISELCRNIFLEKGFRNKKVHCPNGFENFKVDEKLIKDESDGCKSVITIDQLESHLRECEFVFIECPNDPIRCKDKFRKNQTDKHMNKCQYVTINCEHCKEPVIKNDMPTHIENDCSIISLECEFCKDKFGRRSLENHIANECPNVVIDCPHKEGGCTARIKRCYLSQHLTEEDNHIRYMQKIIEKHRIQVEESDRIIKKLRVDYKELEKRVEITSRYKGNWTIENWSQKLTHYPNNERLKSPYFCIGSKSFYVGLYPNGFNQTNAGFMSIFLHLYEKPSTSTTVVRFSFELLHSDPTKSLKFEKSNKYTENKGSGFSQFIDIKMINNFIIDGKLTINIDVEVIPPSSSLVTK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The presence of MATH domains is critical for the interaction of the designed protein with its intended targets.2. The protein can be expressed in cytoplasm and perform zinc ion binding without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "ILFLTDLHWDHDYLEGTDPDCADPLCCRRGSGLPPASRPGAGYWGEYSKCDLPLRTLESLLSGVGLAGPFDMVYWTGDIPAHDVWQQSRQDQLRALNTITALVRKFLGPVPVYPAVGNHESTPVNSFPPPFIEGNHSSRWLYEAMAKAWEPWLPAEALRTLRIGGFYALSPRPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVGELQAAEDRGDKVHIIGHIPPGHCLKSWSWNYYRIVARYESTLAGQFFGHTHVDEFEVFYDEETLSRPLAVAFLAPSATTYIGLNPGYH",
    "response#2": "ILFLTDLHWDHDYLEGTDPDCADPLCCRRGSGLPPASRPGAGYWGEYSKCDLPLRTLESLLSGVGLAGPFDMVYWTGDIPAHDVWQQSRQDQLRALNTITALVRKFLGPVPVYPAVGNHESTPVNSFPPPFIEGNHSSRWLYEAMAKAWEPWLPAEALRTLRIGGFYALSPRPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVGELQAAEDRGDKVHIIGHIPPGHCLKSWSWNYYRIVARYESTLAGQFFGHTHVDEFEVFYDEETLSRPLAVAFLAPSATTYIGLNPGYDFRLGHIKNCATWLTHSSFFQSLTETENLLILKSTWHVWSWLELLSVSVEIFGNQVCEEKIVFLSEKIAVDIVKVFRYILKPLNKQEKRKVEKELNPIFHILFDDVARKLQNLKPSSLEINYMLWQLVWFVAEKVLNEDNLRHGEQYTNQLASDLHNHYKNDLHLEQYAQRVLKMMAIVKSLQKHCL",
    "response#3": "ILFLTDLHWDHDYLEGTDPDCADPLCCRRGSGLPPASRPGAGYWGEYSKCDLPLRTLESLLSGVGLAGPFDMVYWTGDIPAHDVWQQSRQDQLRALNTITALVRKFLGPVPVYPAVGNHESTPVNSFPPPFIEGNHSSRWLYEAMAKAWEPWLPAEALRTLRIGGFYALSPRPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVGELQAAEDRGDKVHIIGHIPPGHCLKSWSWNYYRIVARYESTLAGQFFGHTHVDEFEVFYDEETLSRPLAVAFLAPSATTYIGLNPGYYDRGELLGSNT"
  },
  {
    "reference": "MEGDSYHNATTVNGTPVNHQPLERHRLWEVITIAAVTAVVSLITIVGNVLVMISFKVNSQLKTVNNYYLLSLACADLIIGIFSMNLYTTYILMGRWALGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTPKRAGIMIGLAWLISFILWAPAILCWQYLVGKRTVPLDECQIQFLSEPTITFGTAIAAFYIPVSVMTILYCRIYRETEKRTKDLADLQGSDSVTKAEKRKPAHRALFRSCLRCPRPTLAQRERNQASWSSSRRSTSTTGKPSQATGPSANWAKAEQLTTCSSYPSSEDEDKPATDPVLQVVYKSQGKESPGEEFSAEETEETFVKAETEKSDYDTPNYLLSPAAAHRPKSQKCVAYKFRLVVKADGNQETNNGCHKVKIMPCPFPVAKEPSTKGLNPNPSHQMTKRKRVVLVKERKAAQTLSAILLAFIITWTPYNIMVLVSTFCDKCVPVTLWHLGYWLCYVNSTVNPICYALCNRTFRKTFKMLLLCRWKKKKVEEKLYWQGNSKLP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins; Primary transducing effect is Pi turnover.2. A protein that can perform neurotransmitter receptor activity, G protein-coupled acetylcholine receptor activity, phosphatidylinositol phospholipase C activity, G protein-coupled serotonin receptor activity in postsynaptic membrane, dendrite, plasma membrane to support gastric acid secretion, chemical synaptic transmission, G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger, regulation of phosphatidylinositol dephosphorylation.3. The protein design should prioritize the formation of helix-helix-helix-helix-helix-turn-helix-helix-helix-helix-helix-helix-strand-helix-helix elements.4. The protein should exhibit Polar residues compositional bias.5. The protein should contain Helical; Name=4, Helical; Name=5, Helical; Name=7, Helical; Name=2, Helical; Name=3 transmembrane regions to facilitate transport across the membrane.6. The Cytoplasmic, Extracellular topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "GNVMVMISFKIDKQLQTISNYFLFSLAIADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYRAKRTTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFYFPVTIMCFLYWRIWRETKKRQKDLPNLQAGKKDSSKRSNSSDENTVVNHASGGLLAFAQVGGNDHDTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIKKSYTCFGSIKEWCIAWWHSGREDSDDFAYEQEEPSDLGYATPVTIETPLQSSVSRCTSMNVMRDNYSMGGSVSGVRPPSILLSDVSPTPLPRPPLASISQLQEMSAVTASTTANVNTSGNGNGAINNNNNASHNGNGAVNGNGAGNGSGIGLGTTGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGASSNAANGGGGGPGAGAAASASLSMQGDCAPSIKMIHEDGPTTTAAAAPLASAAATRRPLPSRDSEFSLPLGRRMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKRQESKAAKTLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTISGNALVILAVLTSRSLRAPQNLFLVSLAAADILVATLIIPFSLANELLGYWYFRHTWCEVYLALDVLFCTSSIVHLCAISLDRYWSVSRALEYNSKRTPRRIKGIILTVWLIAAFISLPPLIYKGDKGKKPGGRPQCKLNEEAWYILSSSIGSFFAPCLIMILVYLRIYLIAKRRNRQGPHGKQAPGDGDTGPSALGGTSTISKLPPSILPAVGEANGHSKPPGEREGGEQMGDPTSPSTPPNQSSVGPEDGSQKQEEEEEEEEEEEEECGPPAPPTSSSLQGTPNFQPSQGSQVLATLRGQVLLARGPASLGLQPWRRRTQMNREKRFTFVLAVVIGVFVLCWFPFFFSYSLGAICPQHCKVPHGL",
    "response#2": "GNSFVVWSILKRMQKRSVTALMVLNLALADLAVLLTAPFFLHFLAQGTWSFGLAGCRLCHYVCGVSMYASVLLITAMSLDRSLAVARPFVSQKLRTKAMARRVLAGIWVLSFLLATPVLAYRTVVPWKTNMSLCFPRYPSEGHRAFHLIFEAVTGFLLPFLAVVASYSDIGRRLQARRFRRSRRTGRLVVLIILTFAAFWLPYHVVNLAEAGRALAGQAAGLGLVGKRLSLARNVLIALAFLSSSVNPVLYTLSTKQFRRAFRRILRSGAWGIVLEAVAGAGIVTTFVLTIILVASLPFVQDTKKRSLLGTQVFFLLGTLGLFCLVFACVVKPDFSTCASRRFLFGVLFAICFSCLAAHVFALNFLARKNHGPRGWVIFTVALLLTLVEVIINTEWLIITLVRGSGEGGPQGNSSAGWAVASPCAIANMDFVMALIYVMLLLLGAFLGAWPALCGRYKRWRKHGVFVLLTTATSVAIWVVWIVMYTYGNKQHNSPTWDDPTLAIALAANAWAFVLFYVIPITPCVLISFLASLAFYLPAESGEKMSTAISVLLAQAVFLLLTSQRLPETALAVPLIGKYLMFIMSLVTGVIVNCGIVLNFHFRTPSTHVLSTRVKQIFLEKLPRILHMSRADESEQPDWQNDLKLRRSSSVGYISKAQEYFNIKSRSELMFEKQSERHGLVPRVTPRIGFGNNNENIAASDQLHDEIKSGIDSTNYIVKQIKEKNAYDEEVGNWNLVGQTIDRLSMFIITPVMVLGTIFIFFFEPIMDS",
    "response#3": "GNVMVMISFKIDKQLQTISNYFLFSLAIADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYRAKRTTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFYFPVTIMCFLYWRIWRETKKRQKDLPNLQAGKKDSSKRSNSSDENTVVNHASGGLLAFAQVGGNDHDTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIKKSYTCFGSIKEWCIAWWHSGREDSDDFAYEQEEPSDLGYATPVTIETPLQSSVSRCTSMNVMRDNYSMGGSVSGVRPPSILLSDVSPTPLPRPPLASISQLQEMSAVTASTTANVNTSGNGNGAINNNNNASHNGNGAVNGNGAGNGSGIGLGTTGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGASSNAANGGGGGPGAGAAASASLSMQGDCAPSIKMIHEDGPTTTAAAAPLASAAATRRPLPSRDSEFSLPLGRRMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKRQESKAAKTLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTILPVG"
  },
  {
    "reference": "MLGGKKILLGITGGIAAYKTTFLVRLLIKAGAEVKVVMTQSASSFVSPLTLSTLSKNPVLMDFIDETDGSISWNNHVELGLWADYMVIAPATANTLSKMAHGTCDNLLLATYLSAKCPVYFAPAMDLDMYKHPSTLESLEKLQSFGNVMIPAESGELASGLHGEGRMAEPENILDFITSNLSKELPLNGKKVLITAGPTHEAIDPVRFLGNRSSGTMGFELAKKAADLGANVILVSGPTSLDISHGSVQLVRITSAQEMYDVCQEHYPDTDVAILAAAVADYRPKHVAKEKIKKKEGDLQIELERNPDILYSLGKEKKNQFLVGFALETENELENAKGKLQRKNLDAIVLNSLKDEGAGFGGNTNKITFIDKNSGIKTFALKTKAEVASDIWEEITSRIHA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Mg(2+) is required for the proper folding and stability of the protein.2. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A3. The protein should be designed to catalyze the following reaction H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. The designed protein must possess phosphopantothenoylcysteine decarboxylase activity, FMN binding, phosphopantothenate--cysteine ligase activity to support coenzyme A biosynthetic process, pantothenate catabolic process under certain conditions.5. The CTP binding site should be located in a region of the protein that is accessible to the ligand.6. We require a protein that can specifically bind to the metabolites produced in the Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis pathway.7. The designed protein have Flavoprotein domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MSGKHVLITLGPTREKWDAVRFWSNPSSGLMGACIAMAAWLRGAKVTVVSGPVNWWFPEDINVIKVNSAQQMFDAATEVWPSCTTGCFTAAVADFKPIPHGEGKFKKEGSSALRVDFDTNPDILKTIGSMKKDDQQLIGFAAETSNIQEAAKGKLERKNLDLIVANPINKPGAGFESSTNSVYESALKNNKNFILENKKVTITAGCTIEKIDPVRFISNHATGKMGFALAEIAAAMGAQVTLVHGPTFLKPPVDIDVIQVESAHEMMEATHENYPERGILIGAAAVSDYRPAEFSQSKIKKDKNNLTLKLKINPDILFELKKKKTNSIHVGFALETNNEIENAAKKIVQKGLDIIVVNNPAEDGAGFAVDTNKVKFFDKNGNTTYLPKMSKKEVAKDILKIIINKL",
    "response#2": "MSGKHVLITLGPTREKWDAVRFWSNPSSGLMGACIAMAAWLRGAKVTVVSGPVNWWFPEDINVIKVNSAQQMFDAATEVWPSCTTGCFTAAVADFKPIPHGEGKFKKEGSSALRVDFDTNPDILKTIGSMKKDDQQLIGFAAETSNIQEAAKGKLERKNLDLIVANPINKPGAGFESSTNSVYEGLHVVITSGPTYEPIDDVRFLGNRSSGKMGLALASAAVRMSARVSLVTGPTHGVPHPRVETTRVTTAQEMLDATAALCGEADLLIAAAAVADFRPQAKAAGKIRREGPVTLTLEPTPDIVATVTERYPKLAAVGFAAEPGNDLESARRKLEEKRLIAIFLNDVSRPDIGFESDQNEGVAVFVEGNAYMSGKHVLITLGPTREKWDAVRFWSNPSSGLMGACIAMAAWLRGAKVTVVSGPVNWWFPEDINVIKVNSAQQMFDAATEVWPSCTTGCFTAAVADFKPIPHGEGKFKKEGSSALRVDFDTNPDILKTIGSMKKDDQQLIGFAAETSNIQEAAKGKLERKNLDLIVANPINKPGAGFESSTNSVYLFDDDGTHPLDKSILLIVGGGIAAYKSLELVRRLAERGVKTRAILTKAGAEFVTPLSLSALTGQKIYSDLFSLTDEAEMGHIELSRSADLVVVAPATADLMAKMANGLANDLASTTLLATDKTVLMAPAMNVRMWESAAAKRNRATLEKDGVLFVGPNDGEMACGEFGPGRMAEPLEIVTAIEQLARYLQ",
    "response#3": "MSGKHVLITLGPTREKWDAVRFWSNPSSGLMGACIAMAAWLRGAKVTVVSGPVNWWFPEDINVIKVNSAQQMFDAATEVWPSCTTGCFTAAVADFKPIPHGEGKFKKEGSSALRVDFDTNPDILKTIGSMKKDDQQLIGFAAETSNIQEAAKGKLERKNLDLIVANPINKPGAGFESSTNSVYLENKKVTITAGCTIEKIDPVRFISNHATGKMGFALAEIAAAMGAQVTLVHGPTFLKPPVDIDVIQVESAHEMMEATHENYPERGILIGAAAVSDYRPAEFSQSKIKKDKNNLTLKLKINPDILFELKKKKTNSIHVGFALETNNEIENAAKKIVQKGLDIIVVNNPAEDGAGFAVDTNKVKMVFKDGSVKLSGKRLLVTAGPTYEAIDPVRFVGNYSSGKMGFAIAGELARRGAFVDLISGPVHITVSHPLITRIDVNSAIEMYNECVARFPSSDGAVMSAAVSDFRPQEVAEYKIKRSGDNMLLNLVPNPDIAAELGKMKRPNQLLVGFALETNNEVANAQKKLESKNLDFIVLNSLAEKGAGFMSDTNKISIITKDGVEDLPLMSKDEVADKILDKAYALL"
  },
  {
    "reference": "MTKEILFATGNQGKAKELKEAFKRAGVGVEIKTNADLDTPPHPIESGRTFEANAKIKAHELADFSGLPTIADDSGLMVDALNGEPGVRSARYAGEAHNDAKNNAKLLANLGGVPDEKRTAKFWTTIVVSMPGEFDKDLVVSGTCSGRILAAPRGEDGFGYDPLFFIPEKGKTFAQMTTDEKNEISHRGNAVRKLLQELPDWLKQFN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. We are looking for a protein with broad substrate specificity for dITP + H2O = dIMP + diphosphate + H(+).2. The protein should be able to bind Mg(2+) ligand in a variety of conditions3. The protein must exhibit metal ion binding. The designed protein sequence is ",
    "response#1": "MAWSRL",
    "response#2": "MFPN",
    "response#3": "MRL"
  },
  {
    "reference": "QKGLDYACQQGVAVLQKELEKIRIPDVSGKFKLRPFGKGHYNFHSLVVRSFQLPNPQIRLQPNVGLRVSISNANVRIGGRWKARKGFIKVRGKFDLSVEGVSISADLKLGSVPASGRATVTCSSCSSNINRARLRSQASWGGWLKLFHKRIESSLRNTMNSKICQVLTSSVSSKLQPYVETLPLKERLDSVAGIDYSLVAPPRATADSLDMQLKGEFYNVARPSPPPFMPPPMAIPSLHDRMIYLAISDYLFNTAALVYQQAGAFGLTLRDDMIPKESKSRLTTKFLGKALPQVAKMFPNMNVQLTLSVSSPPHLTTRPTGIALTAAVDLQAFAILPNSSLASLFLLGLKLNTSAKIGTKADKLVGELTLGRLILELKHSNIGSFPVQLLQALMDYVLSAVVLPKVNEKLQRGLPLPMPRKVQLYDLVLQPHQDFLLLGANVQHG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that can perform lipid binding in extracellular space, membrane to support defense response to bacterium, innate immune response.2. The protein must exhibit the following characteristics: The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope.. The designed protein sequence is ",
    "response#1": "ETLDVQMKGEFYSENHHNPPPFAPPVMEFPAAHDRMVYLGLSDYFFNTAGLVYQEAGVLKMTLRDDMIPKESKFRLTTKFFGTFLPEVAKKFPNMKIQIHVSASTPPHLSVQPTGLTFYPAVDVQAFAVLPNSSLASLFLIGMHTTGSMEVSAESNRLVGELKLDRLLLELKHSNIGPFPVELLQDIMNYIVPILVLPRVNEKLQKGFPLPTPARVQLYNVVLQPHQNFLLFGADVVC",
    "response#2": "ETLDVQMKGEFYSENHHNPPPFAPPVMEFPAAHDRMVYLGLSDYFFNTAGLVYQEAGVLKMTLRDDMIPKESKFRLTTKFFGTFLPEVAKKFPNMKIQIHVSASTPPHLSVQPTGLTFYPAVDVQAFAVLPNSSLASLFLIGMHTTGSMEVSAESNRLVGELKLDRLLLELKHSNIGPFPVELLQDIMNYIVPILVLPRVNEKLQKGFPLPTPARVQLYNVVLQPHQNFLLFGADVVP",
    "response#3": "SLDMQLKGEFYNVARPSPPPFMPPPMAIPSLHDRMIYLAISDYLFNTAALVYQQAGAFGLTLRDDMIPKESKSRLTTKFLGKALPQVAKMFPNMNVQLTLSVSSPPHLTTRPTGIALTAAVDLQAFAILPNSSLASLFLLGLKLNTSAKIGTKADKLVGELTLGRLILELKHSNIGSFPVQLLQALMDYVLSAVVLPKVNEKLQRGLPLPMPRKVQLYDLVLQPHQDFLLLGANVV"
  },
  {
    "reference": "METSGEKRMTGTEKKLGLLASDYGLSAIEKDYPVLGCAVDSRKVEAGFIFGAFPGTTVNGEDFIEKAILAGAIAIVARPEVNIDGAVHLAAENPRQAFAEIAAHFYAPFPPVMAAVTGTNGKTSVAELCRQLWTMAGHKAASIGTLGVITENTQYSLGMTTPDIVTFLSGCADLAKSGVSHLIFEASSHGLDQYRSDGAKVIAGAFTSFSRDHLDYHETMERYLEAKLRLFDERVALDGTAVVWADDPAAETVIAHIRKRGLNLIDIGKNAEAIRILDVQADSKGQDITLSIKDKPYQIRLPLIGGYQLSNALVAAGLVMATGGDVEETMAALSRLKPVRGRLERATDTEQGAEIYVDYAHTPDGLRAAIEALRPHTEGRLLVVFGAGGDRDRGKRPEMAKIAASLADHVIVTDDNPRGEDAAAIRKEVLVGAPLAEEIGGRKKAIFSAVRQAHKGDIVLIAGKGHEQGQIIGRGDDMRVLPFDDVTVAKKAVL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a fast turnover rate for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.2. The designed protein should be optimized for ATP binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity activity in order to improve cell cycle. The designed protein sequence is ",
    "response#1": "MRITGITLDSRSVRPGDLWAALPGARAHGADFADQAASRGAACALTDAQGAQRCADAGLPALVIQDPRAATAQAAAAVAEWFFERPSDEMTVIGVTGTNGKTTISQLLAQWANLLGHQAGVMGTTGNGLLGQIVPAANTTGSAIEIPHVLAEMVQQGADYAAMEVSSHGLVQGRISALQFQASIFTNLSRDHLDYHGDMASYAGAKKTLFTAHHCGKAIINADDAVGREWLLTLPEAVAVAQEKISLSGWAGQALWLEQVHYSTQGVTVRFDSSWGEGEFCAPLVGAFNVMNLMLALATLLALGYDFARLVAVAPDLEPVRGRMQAVDAGQDFLAMVDYAHKPGAVAAVVNTLADYLPQSHGRIGVVLGAGGNRDHDKRPKMGYEAARIAHAVFVTDDNPRDEDPAMIRAAVEEGARDAVAASGREVTVRNIADRREAIREAVAWAQPGDAVVIAGKGHEPYQEVAGQRHPFSDRNVALAALQARR",
    "response#2": "MKLNELINGIKCIQLVGDINKDICTDSREIQAGDIFVCIKGYNSDGHNFIEEARKKKAAFIVSERSLEVSNLTLDSRKVESGSLFIAMRGNKADGRDYVSHAMNSGAAAVLVENSKYGLGYISSNYFNNPAKKLKLIGITGTNGKTTTTYLIEKIMKDYGYKVGLIGTIETIIGNKTIPSNNTTPDALELHRIFREMADAGCEYCVMEVSSHALALDRVYGLNFDVGVFTNLSQDHLDFHKTFENYYNAKAKLFTMCKIGIINIDDEYGKRLIEEYTDNSITYGIDNKADYTAKNLILSKQGVEFTVYTSKGKCKIHTMLIGRFNVYNILAAIGACLAEGIDMDIIKTGIENIEPVAGRMECAAVYRGAYVYIDYAHNPVSLENVLKTLRSYFGGKIFCVFGCGGNRAKSRRIGMGMVSGKMADLTIVTNDNPRFEDPEAIMDDIEDGLNAAGAEYIRIPDRKKAIRYGIVHAVAGDVVLLAGKGHETYQEIKGVKYDMDDRVLVKEVLESLNK",
    "response#3": "ITGTSGKTTTSYLIERALMTEFHVGLIGTTGTRINGNPVPTKLTTPEAPTMQALLARMRDEGVTHVVMEVSSHALELGRVKGIGFDVAAFTNLSQDHLDFHPTMDAYFRAKTLLFTRVQDGTTPRAVICVDDSWGEQMAGVAAQAGCDVTLISTAAEGEAARSAADFYSGGDSWSLRSVSVALTGRQDVSVDVGGEVLDYSISLAGNFNVANSVLAIACARELGVSEAALLKGLESFSGVPGRMECLGGGEQPLAVVDYAHTPDALAQALLALRPLAEQRGGQLWCVFGCGGDRDPIKRPLMGAMAGQYADQVVLTNDNPRNEKPEAILSQILLGLEGHGAVSVQSDRARAITETVRQAAAHDVVLVAGKGDERAQQIADDLNSPFA"
  },
  {
    "reference": "MAAPLHVVILAAGEGKRMKSDTAKVLQKIGGRPMLAHVIATARALDSAGIHVVYGHGGEQVRAAFADQPDLHWAEQAQRLGTGHAVQQAMPGVPQDARVLVLYGDVPLTTPATLQRLLDAPGRLAVLVADLIDPTGYGRIVRDAEGRVGQIVEHKDADEAQREIRTVNTGILVCDGEPLRRWLQNLGNDNAQGEYYLTDVFAAAAAEFSAAEMVHVSDPLEVEGVNDPWQLAQMERAFQRRAARALCEQGARLADPTRFDQRGVVSVGRDVEIDVDVILEGRVELADGVRIGPFCRLKDVTLGPGTEVRAHCDLDGVVVEGAAQIGPYSRLRPGTVLADGVHVGNFVETKNARLGVSSKANHLAYLGDATIGSGVNVGAGTITCNYDGVNKSVTVIEDGAFIGSNSALVAPVTIGAGATIGAGSVIGKNAPEGKLTVARARQVTIDGWQRPVKKPKA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein contains novel MobA-like NTP transferase domains that confer a unique function or activity.2. The protein design should be able to enhance the efficiency of UDP-N-acetylglucosamine biosynthetic process.3. The designed protein should interact with enzymes and substrates within the Nucleotide-sugar biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MESGIIMQLPDTIFIDVRASFEGECLLESGVSILGASQLINAHIKTHSVIEDAIIDHSSVGPMGRIRPQTHLVDTHIGNFVEVKKSTLKGVKAGHLTYLGDATIDEGTNVGAGTITCNYDGKAKYPTVIGKNVFIGSDTQLVAPVTIEDDVMIGAGSTITKNIKQGELALSRAV",
    "response#2": "MESGIIMQLPDTIFIDVRASFEGECLLESGVSILGASQLINAHIKTHSVIEDAIIDHSSVGPMGRIRPQTHLVDTHIGNFVEVKKSTLKGVKAGHLTYLGDATIDEGTNVGAGTITCNYDGKAKYPTVIGKNVFIGSDTQLVAPVTIEDDVMIGAGSTITKNIKQGELALSRAV",
    "response#3": "MESGIIMQLPDTIFIDVRASFEGECLLESGVSILGASQLINAHIKTHSVIEDAIIDHSSVGPMGRIRPQTHLVDTHIGNFVEVKKSTLKGVKAGHLTYLGDATIDEGTNVGAGTITCNYDGKAKYPTVIGKNVFIGSDTQLVAPVTIEDDVMIGAGSTITKNIKQGELALSRAW"
  },
  {
    "reference": "MAQAEVLLTKEPAPQSIDVCELPQKKYEVACNTGAYTSAGLATAGFRTAKYLMDEWFQNSYARYHQAFADRDYSERQRHESKQLAAETGELAYRTQLDSTRRVGERLEDMHCWKSELQREIDELSSETDQMMAQKLRLQRALDALSVPFSIATDNLQCRERRQHPDLVRDYVEVELLKETELIRNIQELLKRTMGQAVGQIRLNREHKENCEINWSDKVEVYNIDDTCARYTNESTQVQFYPHSSKFEESASTPETWGKFTQDVLLRAERERLASVNLRKLIDCILRDTAEDLRLQCDAVNLAFSNRCEELNDARQKLQYHLLKILSEITDQEHQIAALKQAIKDKEAPLRVAQTRLYQRSHRPNVELCRDNAQFRLMSEVEELNMSLKVLKEKLQDAEQALRNLEDSRMSLEKDIAVKTNSLFIDRQKCMTHRNRYPSVLQLAGYQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have a specific function in cilium movement involved in cell motility, cilium assembly, regulation of brood size and should be localized to axonemal microtubule, sperm principal piece, sperm flagellum, microtubule cytoskeleton, sperm midpiece in order to carry out this function efficiently.2. The protein must exhibit the following characteristics: Forms filamentous polymers in the walls of ciliary and flagellar microtubules ; Contributes to normal sperm motility ..3. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "LNIEGLKYKERDGFEMAFTFWQPQPWNVFEVTAYWEQRSRALLGTRYDHRNGAFDWDLSMTLKERGGQQICSQEYRYWRETGVAFVFPEYEHCKPNKTLAVGLVRNGRNFIHRGYVGDIQTGPFCGFGLRTVEERMHHSVHGDNDYRATDITERNLMEFFHELLTQTAYEHDTTRSRRYGSVQLLMTPLLNHQEEDAAGQAAYDKPWIHVPGVTVHFVSPMEMEQLKKGAAHWNNMFDIVFMAYNYYSFLSKDFFQAMRAQSLFILETKLMTVERKDRVQEYESKAKELMKDAGLKV",
    "response#2": "LNIEGLKYKERDGFEMAFTFWQPQPWNVFEVTAYWEQRSRALLGTRYDHRNGAFDWDLSMTLKERGGQQICSQEYRYWRETGVAFVFPEYEHCKPNKTLAVGLVRNGRNFIHRGYVGDIQTGPFCGFGLRTVEERMHHSVHGDNDYRATDITERNLMEFFHELLTQTAYEHDTTRSRRYGSVQLLMTPLLNHQEEDAAGQAAYDKPWIHVPGVTVHFVSPMEMEQLKKGAAHWNNMFDIVFMAYNYYSFLSKDFFQAMRAQSLFILETKLMTVERKDRVQEYESKAKELMKDAGLKK",
    "response#3": "PSGKKVIQYKKAQLEKWSPYLNNNGLVCRLTTYEDQQCTKVLEIKEWYQNREDMLELKHINKTTGLHVDYFKPGHPQALCVHSYKSMLPEMDRVMEFYKKIRVDGLVKREETPMTMTEYYEGRSDFLAYRHVNFGPRVKKLSQSSVESNPRPMVKITERFSRNPEKPADEDVAERLFLIVEERIQLRYHCRDDYITASKREFLRRMEVDSKGNKIIMTPEMCISYEVEPMEHTKKLLYQYETMNQLKNEEKLSRHQAWESELEVLEILKLREEEEEAHTLTIR"
  },
  {
    "reference": "MSSTGSARTEGDSWDLASSVGATATMVAASRALASRGPDPLLDDRFAEPLVRAVGHPFFVRMLDGQIPLDTDDMPMTLQQRREQITVRTRFFDDFFRSATAAGIRQAVILAAGLDARAYRLSWPAGTVVFEVDQPEVISFKTDTLARLGAQPTAERRTVAVDLRDDWPTALRDTGFDSSARTAWIAEGLLPYLPPHAQDRLLDNITALSAPGSRLATENITDMRVFTDERARAMRSTWRQHGLDIDVAELVWLGERRPAGDHLEATGWSVTRLPTERVYADYGFELPDNEILREFRQAISYLSAELG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in methylation, potentially with improved properties such as stability, specificity or selectivity.2. The protein should be able to bind S-adenosyl-L-methionine ligand in a variety of conditions3. For general function, the protein need meet that Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity. The designed protein sequence is ",
    "response#1": "ILDLACGTGEISIRLAEKGFEVTGIDLSEEMLSFAQQKVSSSQPILFLQQDMREITGFDGQFDAVVICCDSLNYLKTKNDVIETFKSVFRVLKPEA",
    "response#2": "VLEFGSGTGNLTAALLAADKNVFGVEPSDAMKKAALQKGIPDVFHDGDFLSFPAPPFEPDTIVSSYAFHHLTDEEKKQAIHTYSNILHSDQ",
    "response#3": "MTSF"
  },
  {
    "reference": "MARFIFITGGVVSSLGKGLLAASLGALLQARGYKARIRKFDPYLNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTGVSAHQSDNVTSGRVYSDIIARERRGDYLGATVQVIPHVTNAIKEFALNDIDDLDFVICEIGGTIGDIESLPFVEALRQLSNDLGRENVVFVHTTLVPYIAAAGELKTKPTQHSVREMTGYGIQPDVLLCRSEHPIPPEERAKIALFCNVSESAVIQALDARSIYDVPLAYHREGLDREVLRAFGIDDAPEPDLKRWIDIMDRFDNPEGEVTIGVVGKYVSLPDAYKSLREALVHGGFANKVKVNIKWLDAEMFESEDEELAAKLEPMHGILVPGGFGERGSEGKIAAIKFARERKIPFFGICLGMQMACIEAARNQAGIANATTTEFGEEGEPIIGLITEWMSEEGLQKRSAGGDLGGTMRLGAYDAKLSPNSAVATQYGTTDISERHRHRYEVNRNYIDQLEKGGLIFSGMSPDGLLPEIVERPDHPWFIGVQFHPELKSRPFDPHPLFKGFIAAALEQSRLV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein with CTP synthase activity, ATP binding, glutaminase activity, metal ion binding is needed to enhance 'de novo' CTP biosynthetic process, glutamine metabolic process in a specific context.2. The designed protein have CTP synthase N-terminal, Glutamine amidotransferase domains with specific sequence motifs that are crucial for its activity.3. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen4. The designed protein should have a binding site that is specific to the target L-glutamine, UTP, Mg(2+), ATP ligand.5. The protein's active site, Nucleophile, Nucleophile; for glutamine hydrolysis must be carefully engineered to ensure that the amino acids involved in H2O + L-glutamine = L-glutamate + NH4(+) are properly positioned for optimal catalysis.6. The protein design should take into account the physiological conditions of the Pyrimidine metabolism, CTP biosynthesis via de novo pathway pathway. The designed protein sequence is ",
    "response#1": "MAQYLFVTGGVVSSLGKGITSASIAVLLEKRGLKVSLQKLDPYINVDPGTMSPFQHGEVYVTEDGAETDLDLGHYERFTHAAVNKYSNATTGSIYSEVIRRERRGDYLGRTVQVIPHITDHIQELILMGAQQGPDVAIVEIGGTVGDIESLPFLEAIRQFGLKQPAGDVCYVHLTLLPFLRASGEMKTKPTQQSVGKLREIGIIPNILVCRCERPMEPGMREKISLFCNVRPEDVIEEVDVDTSIYEVPLNLLRDGLDAALVRHLRLNVRSEPVNMAAWRELVDRIKAPQEALPDAYMSVVEALRHGGLCYDSAIDIKWVNAVELEKGEDIVNEQLACADGILVPGGFGDRGIEGKIRAITYARENKIPFLGICLGMQCAVVEFGRYIGLKKANSSEFDPKTPHPVIDLLPEQKDIEDKGGTMRLGLYPCKMAEGTKAYESYKEGLIYERHRHRFEFNNEYRDLMISKGMIISGTSPNERLVEIIELKDHPWFIGTQFHPEFKSRPNRPHPLFRDFIAAALQQ",
    "response#2": "IFVTGGVASSLGKGLTASSLGMLLRARGLSVTMQKLDPYLNVDPGTMNPFQHGEVFVTDDGAETDLDIGHYERFLDENLDASANVTTGQVYSTVIAKERRGEYLGDTVQVIPHITDELKNRMRRRAQGDDQPDIIITEIGGTVGDIESQPFLEAARQVRQDVGRRNVFYLHVSLIPYIGPSGELKTKPTQHSVAALRGLGIQPDALVLRSDRQVPDTMLRKIGNACDVDHDAVISCPDAPSIYDIPRTLHSQGLDTYILDRFRLPVRETDFAGWEALVERIKHPTDELDAYKSIMESFVHAGAENNCKVVVKPLSAEQVETEEPEKLFSNVSGILVPGGFGERGIEGKIKAVQYARENKIPFFGICLGLQCAVIEFARNVCGIKNAHSSEFKNTKYAVIDLMPEQKNIKNMGATMRLGAYPCVVKEKTNAFEAYKTAYISERHRHRYEVNNKFRSVLTEHGMILSGLSPDKELVEIIELPNHPWFLACQFHPELKSRATNAHPLFREFVLSAVKASA",
    "response#3": "IFVTGGVVSSLGKGIAASSLAAILEARGLNVTMMKLDPYINVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFVRTRLTGRNSITTGKIYESVIRKERRGDYLGATVQVIPHITDQIKHSVYEATRGHDVALVEIGGTVGDIESLPFLEAIRQLRIEHGPEKCLFMHLTLVPFIKAAGEIKTKPTQHSVKELRSIGIQPDILVCRCEQPLPDGERRKIALFTNVPEKAVISAVDLDVIYKLPSWLHKQGLDDIVVRQLGLPFRDVDWTVWGDLLNRVHNPKHEVTVAMVGKYIDLPDAYLSVSEALRAGGFANDARVKIKWVASDLCETPEGAQRELGDVDAICVPGGFGIRGIEGKLGTLRFAREHRIPTLGLCLGLQCMVIEFARSELGLAGASSTEFEPETAHPVVATMDEQKQFVEGAGDLGGTMRLGLYDAQLRPGSVVAGAYGSTEVKERHRHRYEVNNEYRERLEEAGLVISGTSPGGTLVEFVELPADVHPYYVGTQAHPELMSRPTRPHPLFDGLVRAAMEYQKRGLV"
  },
  {
    "reference": "MLIKYVEFIKSATRAAHYPPGDLPEIAFAGRSNVGKSSLINVLVNRKSLVRTSSTPGRTQLINFFDVNGQFTLVDLPGYGFAKVPLAVKKQWGPMMENYLAKRANLRGVILILDIRRTPVAEDIQMLHWLQAYSIKPILVITKCDKVSKNERGKQSRIIAQTLGVETEELNFFSALNREGKDLIWRRIEEVLPAETAGSAGEAPKAT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein have EngB-type G domains with specific sequence motifs that are crucial for its activity.2. The protein requires Mg(2+) to activate a critical catalytic site.3. The protein should have a high affinity for Mg(2+). The designed protein sequence is ",
    "response#1": "MGVEIAFAGRSNVGKSTLVNALTGRKTLARASSQPGRTKQLNFFNLADQLVLVDMPGYGYAQAAKDVKEDWQGLMFSYLRGRPNLRRVMLLVDARVEFKASDIAVMELLDKAAVTFQLVVTKADAVKPTPLQRRVADVYAAARAHPAAHPHVFVTSSDTGSGIAELRAALAGLTA",
    "response#2": "LVLGGAPGFGGAALLAAEACARLGVGKISLATAPEHVTASLVRRPEVMVHGVRGAGDLGELPQQADVVVVGPGLGQGSWGQGMLQSALAGDGPLVVDADALNLLATRFSGQYRDDWILTPHPGEAARLLDLSASDVQADRPAAALALQRRYGGVVVLKGAGSLVAGPEGLAVCPFGNPGMASGGMGDTLSGILGALVAQGLPLETAARLGVLVHALAADMAARQAGERGLLAGDLAS",
    "response#3": "MLPVIALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGQANYDGYEFIVVDTGGIDGSEEGIEIEMADQSLLAIEEADIVLFLVDARVGMTVADQAIANHLRKQEKKCFVVANKTDGIDADSNCAEFYQLSLGEIHHIAASHGRGITLLLEQTLQPLIAELAALDEDVSNDDEC"
  },
  {
    "reference": "MTIHTDTRPTLNLIRLAHGRGLDLPAYETKGAAGMDLRAAIEDGTTLTLAPGKRALVPSGFIFEIPEGFEAQIRPRSGLAFKNGITCLNSPGTVDSDYRGEVKVLLINHGDEPFEITRGMRIAQVVIAPVTQVRVAEITEVSDTARGAGGFGSTGV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be able to bind substrate ligand in a variety of conditions2. The protein must exhibit the following characteristics: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA..3. A protein that is capable of catalyzing reactions within the Pyrimidine metabolism pathway with high efficiency and specificity.4. The designed protein should have dUTP diphosphatase activity to facilitate dUTP catabolic process, dUMP biosynthetic process. The designed protein sequence is ",
    "response#1": "LPKYFTDGSSGMDLRACIDKEINLNPNETVLISTGVSIDISDQKISGLILPRSGLSHKYGIILSNTIGLIDSDYQGPIMISMLNRSNKNFLISIGDRIAQIIFVPLIQIEWNIVNKFVKITNRKDMGFGHSG",
    "response#2": "LLPVYATTHAAGMDVAACLEENVVVLPFETALIPSGFAIELPEGYEAQLRPRSGLALRHLISLPNTPATIDADYRGEVKIILINYGQEPFVVHHGDRVAQMVVAKVERVFFDEVLTLASSSRADGGFGHTGR",
    "response#3": "LPRYMSEHAAGMDICAAVADEVVILPGERALIPTGIAIALPEGFEAQIRPRSGLALKHGVTLVNAPGTIDADYRGEIGVLLINHGNDPFVVARGSRVAQMVIAPVCRVAWSESGSLETTTRGDGGFGHR"
  },
  {
    "reference": "MDEDLMGFFDFSKEFKRTEAPKGCISSNFVGLGTEKEKTKPPKQENLGTEYVKEIVDREKQNLESLGIGGSAAKRNRTLDDIDSDNEECYEAPDLRLDEEFYSKYYFDLNRDKTLPIYTQRDQIMKAIRENTVVILKGETGCGKTTQVPQYIIDEAYQNRQYCNIVVTQPRRIAAISIANRVSQERHWEPGTVCSYQVGLHRQSGSEDARLLYCTTGVLLNFLINHKTLTHYTHIVLDEVHERDQEMDFLLIVVRRLLATNSRHVKVILMSATIDSREFVQYFATKNGIPPVINASHGRKYPLVKFYRDQLKNMQWQEDQPNPDEPGMGSHGYSAAIKILLVIDNMERRTEGQSQRSYEENLKTGSVLIFLPGINEIDNMAESIDHVMQENPALKVSIIRCHSLMTPDSQRDVFASPPVGYRKIILTTNIAESSITVPDVSYVIDFCLAKVLVTDTATNFSSLRLVWASKSNCRQRAGRVGRLRSGRVYRMVPKSFYMKHMLEFGVPEMLRSPLESSVLKAKELNMGPPIEMLALALSPPKLSDIRNTILLLKEVGALYPTVDGNYVELDGDLTPWGSIMTRLPLDIRLSRLVLLGYVFNCLDEAIVMAAGLSVRGLYLQEAGFQSYEAYWMHYVFADGSSSDLVAIWRFYKTYLNMCENRIMQESAAQWARRYHISLRSLKEMHLLVQELQYRCNKLRLHPVQLQSCQIKDDRERALILKILIAGAFYPNYFIRSNKFNPDYGRNTYQVLGGYDPCRTVYFTHFEPR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein contains novel Helicase ATP-binding, Helicase C-terminal, Tudor domains that confer a unique function or activity.2. The protein must exhibit the following characteristics: Involved in telomeric trans- silencing, a repression mechanism by which a transposon or a transgene inserted in subtelomeric heterochromatin has the capacity to repress in trans in the female germline, a homologous transposon, or transgene located in euchromatin; Probable ATP-binding RNA helicase which plays a central role during spermatogenesis and oogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity; Required for anteroposterior and dorsoventral axis formation during oogenesis .; Also involved in telomere regulation by repressing specialized telomeric retroelements HeT-A, TAHRE, and TART; Drosophila telomeres being maintained by transposition of specialized telomeric retroelements; Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi and govern the methylation and subsequent repression of transposons.3. A protein that contributes to oogenesis, spermatogenesis, RNA-mediated gene silencing by functioning in cytoplasm with ATP binding, ATP hydrolysis activity, RNA helicase activity, nucleic acid binding.4. Incorporating a DEAH box motif is critical for the successful design of the protein. The designed protein sequence is ",
    "response#1": "QSRQSYDEALRYGAVLIFLPGIYEIDTMAENLTCMLENDPNIKVSIVRCFSLMTPENQRDVFNPPPPGFRKIILTTNIAESSITVPDVSYVIDFCLAKVKVTDTASSFSSLRLTWASKANCRQRAGRVGRLRSGRVYRMVNKHFYQREMPEFGIPEMLRLPLQNSVLKAKVLKNLPIYEQREDILAAIRENPVVILKGETGCGKTTQVPQYILDEAFKRREFCNIVVTQPRRIAAISIANRVCHERKWQPGTVCSYQVGLHRQSNLEDTRLLYCTTGVLLNNLVRVKTLTQYTHIILDEVHERDQDMDFLLLVVRRLLALNSRHVKVILMSATIDTREFSKYFAMSSSLPPVVTASHGRKFPLVKFKNLPIYEQREDILAAIRENPVVILKGETGCGKTTQVPQYILDEAFKRREFCNIVVTQPRRIAAISIANRVCHERKWQPGTVCSYQVGLHRQSNLEDTRLLYCTTGVLLNNLVRVKTLTQYTHIILDEVHERDQDMDFLLLVVRRLLALNSRHVKVILMSATIDTREFSKYFAMSSSLPPVVTASHGRKFPLVKFFSRYGAVLIFLPGIYEIDTMAENITLMLENDRNVKVFIVRCFSLMTPENQRDVFHPPPPGFRKIILTTNIAESSITVPDVSYVIDFCLTKVLVTDTATNFSSLRLTWASKANCRQRAGRVGRAGRAGVAVTLFTEKGQEFGDSLEYKPSIIIFLPGINEIEKMEGALEKLIASIQNAAQRPNLLIMKLHSTLPADDQTAVFRKPGPNQRKVILSTNIAESSITVPDIKFVIDFCLQRILVTDTLTNFSTLRTEWASKSNCIQRAGRAGRLMSGRVYRLVDRRFFENNMDVSTSPEILRCPLETVVLKAKLL",
    "response#2": "KIIMVIDNMEREGAIHSRMSYDEALRYGAVLIFLPGIYEIDTMAENITLMLENDRNVKVFIVRCFSLMTPENQRDVFHPPPPGFRKIILTTNIAESSITVPDVSYVIDFCLTKVLVTDTATNFSSLRLTWASKANCRQRAGRVGRLRSGRVYRMVNKSFYQREMSEFGIPEMLRLPLQNSVLRAKELKNLPIYAKREEILAAINANPVVILKGETGCGKTTQVPQYILDEGYKSGKYCNIVVTQPRRIAAISIANRVCQEREWQQDTVCSYQVGLHRPTSLEDTRLLYCTTGVLLNNLIRNKTLTHYTHIVLDEVHERDQDMDFLLIVVRRLLATNSRHVKIILMSATIDARELSDYFTTTNSIPPVISASHGRKHQSQQSYEEAKRTGAVLIFLPGIHEIDTMAEHIGRIVDENPNFKISIVRCHSLMSPDSQEEVFLPPPLGHRKVILTTNISESSITVPDVSYVIDFCLTKVLHTDTASNFSSLRLEWASKVNCRQRAGRVGRLRSGRVYRMVTKDFYMNHMNEFGIPEMLRSPLQNSVLKAKELSKTITGLITGIINCGKFYFQPLSLAECIRNMSEIFNAPQQLRKYVVDACDISKGMMVLAKRDSNFQRATVIRPENQSNRQPMFYVRFIDYGDCALLPMQQLRFMSEELIQQYGDLPPRVFECRLKIIMVIDNMEREGAIHSRMSYDEALRYGAVLIFLPGIYEIDTMAENITLMLENDRNVKVFIVRCFSLMTPENQRDVFHPPPPGFRKIILTTNIAESSITVPDVSYVIDFCLTKVLVTDTATNFSSLRLTWASKANCRQRAGRVGRLRSGRVYRMVNKSFYQREMSEFGIPEMLRLPLQNSVLRAKEL",
    "response#3": "KIIMVIDNMEREGAIHSRMSYDEALRYGAVLIFLPGIYEIDTMAENITLMLENDRNVKVFIVRCFSLMTPENQRDVFHPPPPGFRKIILTTNIAESSITVPDVSYVIDFCLTKVLVTDTATNFSSLRLTWASKANCRQRAGRVGRLRSGRVYRMVNKSFYQREMSEFGIPEMLRLPLQNSVLRAKELLDKIRSNAVVVLQGATGCGKTTQVPQYLLEEAFERKEYCNIIVTQPRKIAAISIARRVAQERKCDLGTLVGYKVGLKEQHNEDTRLLYVTTGVLLQSLINSKTMATYTHVILDEVHEREVDMDFLLIVVRRLLSTNSKKTKVILMSATIDAKGFSEYFKIPKKSGYKIIMVIDNMEREGAIHSRMSYDEALRYGAVLIFLPGIYEIDTMAENITLMLENDRNVKVFIVRCFSLMTPENQRDVFHPPPPGFRKIILTTNIAESSITVPDVSYVIDFCLTKVLVTDTATNFSSLRLTWASKANCRQRAGRVGRLRSGRVYRMVNKSFYQREMSEFGIPEMLRLPLQNSVLRAKELKNLPIYAKREEILAAINANPVVILKGETGCGKTTQVPQYILDEGYKSGKYCNIVVTQPRRIAAISIANRVCQEREWQQDTVCSYQVGLHRPTSLEDTRLLYCTTGVLLNNLIRNKTLTHYTHIVLDEVHERDQDMDFLLIVVRRLLATNSRHVKIILMSATIDARELSDYFTTTNSIPPVISASHGRKHEILAAINANPVVILKGETGCGKTTQVPQYILDEGYKSGKYCNIVVTQPRRIAAISIANRVCQEREWQQDTVCSYQVGLHRPTSLEDTRLLYCTTGVLLNNLIRNKTLTHYTHIVLDEVHERDQDMDFLLIVVRRLLATNSRHVKIILMSATIDAREL"
  },
  {
    "reference": "MLKGKHILLGITGSIAAYKAAFLIRSLVKEGAEVKVIMTDMAKRFITPLTIATLSKNPVLTEFYNPENGDWNSHVSLGIWADLYIIAPASANTIAKMATGVADNLLLTSYLSARCPVMVAPAMDLDMYRHPSTTKNLETLSTRGVMIAEPSSGELASGLDGKGRMEEPEIIVEHIKKIFNTSPALSGKKILITAGPTREEIDPVRYITNHSSGKMGYAIAQECAERGALVTLISGPANIEISSPLINKIEVVSAAEMYQNTVTAYESGTDVVILSAAVADFTPVNTEAKKIKRGKENYSLELKPTKDIAAELGNQKRENTIHIGFALETDNELANANDKLHRKNLDAIVLNSLKDEGAGFGTDTNKVCIIGRDGSKNDYNLKSKRQVASDIVDYIEKLLSC",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein contains novel DNA/pantothenate metabolism flavoprotein C-terminal domains that confer a unique function or activity.2. The designed protein must possess FMN binding, metal ion binding, phosphopantothenoylcysteine decarboxylase activity to support coenzyme A biosynthetic process under certain conditions.3. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A4. The protein have enhanced H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2. The designed protein sequence is ",
    "response#1": "MSGGIAAYKACHLVRDLKEAGDDVRVVPTPSALNFVGAATFEALSGHPVSTTVFDAVDEVQHVRVGQEADAVVIAPATADVMARLVAGRADDLLTATVLVAQCPVIVVPAMHTEMWWNPATQANVRTLRERGVIVLEPAHGRLTGRDTGPGRLPEPDQIAELAARVLDGTLAGRRVLVTAGPTREAIDPVRFVSNRSSGRMGYALAEVAWRRGAAVTLVSGPTALPPPVGVELVRVETAAAMAEAVAAHLPAADVLVMAAAPADFRPAAPAAAKLTKEDGLPPPLAWALTADILQSTRPLRRPDTRVVGFALETADDALARARAKLEAKGLDLVVLNRADEADAGFDTETNRVTVLAADGTVTAWPEMGKRDLADRLLDLVAGLS",
    "response#2": "MSGGIAAYKACHLVRDLKEAGDDVRVVPTPSALNFVGAATFEALSGHPVSTTVFDAVDEVQHVRVGQEADAVVIAPATADVMARLVAGRADDLLTATVLVAQCPVIVVPAMHTEMWWNPATQANVRTLRERGVIVLEPAHGRLTGRDTGPGRLPEPDQIAELVAGILERHALAGRRVLVTAGPTREAIDPVRFVSNRSSGRMGYALAEVAWRRGAAVTLVSGPTALPPPVGVELVRVETAAAMAEAVAAHLPAADVLVMAAAPADFRPAAPAAAKLTKEDGLPPPLAWALTADILQSTRPLRRPDTRVVGFALETADDALARARAKLEAKGLDLVVLNRADEADAGFDTETNRVTVLSRDGHARDWPVMPKTGVAAAIWDAVAPLL",
    "response#3": "MSGGIAAYKACHLVRDLKEAGDDVRVVPTPSALNFVGAATFEALSGHPVSTTVFDAVDEVQHVRVGQEADAVVIAPATADVMARLVAGRADDLLTATVLVAQCPVIVVPAMHTEMWWNPATQANVRTLRERGVIVLEPAHGRLTGRDTGPGRLPEPDQIAELAARLLGG"
  },
  {
    "reference": "MDDTQHFCLRWNNYQSSITSAFENLRDDEAFVDVTLACEGRSIKAHRVVLSACSPYFRELLKSTPCKHPVILLQDVNFMDLHALVEFIYHGEVNVHQKSLQSFLKTAEVLRVSGLTQQQAEDTHSHLAQIQNLANSGGRTPLNTHTQSLPHPHHGSLHDDGGSSTLFSRQGAGSPPPTAVPSLPSHINNQLLKRMAMMHRSSAAAAAEETSHAFKRLRGSDNSLPLSGAVGSGSNNNSPDLPPLHARSASPQQTPADFSTIKHHNNNNTPPLKEEKRNGPTGNGNSGNGNGNGNGASNGNGISISDKLGSLTPSPLARAGADDVKSEPMDMVCSNNNANANDEHSNDSTGEHDANRSSSGDGGKGSLSSGNDEEIGDGLASHHAAPQFIMSPAENKMFHAAAFNFPNIDPSALLGLNTQLQQSGDLAVSPQGKLTTNATTTTTTINNSITNNNNNNNNNNYDYSLPTKNSNSQKTPSPTTTTLTTPTTTTPTRPTAITSASGICGLNLSTFAANGSSSGGSNGGLSMTALLPQQQQQQQQQHQMSQQQQQQQQQQQQQGNSSSGQQQQPNGILACSTPKANTPTTTQQQMYAAVMAAAASASASTSGSANSSLNNSNSTLNTSGGLNNSASGGDDFRCNPCNKNLSSLTRLKRHIQNVHMRPTKEPVCNICKRVYSSLNSLRNHKSIYHRNLKQPKQEPGVGATQAAANSFYHQQHQQQQLNHHSSS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein with metal ion binding, DNA binding is needed to enhance response to ecdysone in a specific context. The designed protein sequence is ",
    "response#1": "PIVHLKGESNSLKCLRYRLKPYKELYSSMSSTWHWTSDNKNSKNGIVTVTFVTEQQQQMFLGTVKIPPTVQISTGFC",
    "response#2": "VTQRRTVRAFTDEPVDPGAVRRAVAAAVTAPAPHHTTPWRFVLLESGESRTRLLDAMRDAWIADLRRDGKSEESVAKRVRRGDVLRNAPYLVVPCLVMDGSHTYGDVRRDAAEREMFVVATGAGVQNLLVALAGERLGSAWVSSTMFCRDVVREVLGLPDGWDPMGAVAVGV",
    "response#3": "VEIGANAFALFTKNQRQWQAPPLKEDTIEKFKRFCQVHHFSPAQILPHDSYLINLGNPEAENLAKSREAFIDEMRRCDQLGLTLLNFHPGSHLNKISEQDCLARIAESINIAVDSVPNVVAVIENTAGQGSNLGWRFEHLAEIIEQVENKQRVGVCLDTCHLFSAGYDISSLASCEQTFADFDKVVGFEFLRGMHLNGSKTLLASRVDRHHTLREGTIGTDVFKFIMNNAHFDNIPLILETIEPEIWAEEINFLRR"
  },
  {
    "reference": "XYEGYDSTANPTVSNVFSTAAFRFGHATIHPLVRRLDASFQEHPDLPGLWLHQAFFSPWTLLRGGYNEWREFCGLPRLETPADLSTAIASRSVADKILDLYKHPDNIDVWLGGLAENFLPRARTGPLFACLIGKQMKALRDGDWFWWENSHVFTDAQRRELEKHSLSRVICDNTGLTRVPMDAFQVGKFPEDFESCDSITGMNLEAWRETFPQDDKCGFPESVENGDFVHCEESGRRVLVYSCRHGYELQGREQLTCTQEGWDFQPPLCKDVNECADGAHPPCHASARCRNTKGGFQCLCADPYELGDDGRTCVDSGRLPRVTWISMSLAALLIGGFAGLTSTVICRWRVLGWKAGILTGCREPSEGKVAGHCRTASCSQNHRTTLFQTQANPKSAGRLFSQHG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The ideal protein would be one that plays a critical role in response to oxidative stress, thyroid hormone generation through iodide peroxidase activity, calcium ion binding within membrane.2. The protein must be engineered to have a high affinity for heme b in order to increase its catalytic activity.3. The designed protein should have at least Helical transmembrane domains to ensure proper localization.4. The protein must exhibit the following characteristics: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4)..5. The designed protein must have at least one EGF-like, Sushi domain. The designed protein sequence is ",
    "response#1": "YDGWYNNLAYHRRGTAGAPLMRFLPARYSDGAYQPLRGLPNARKISNAATRGDSALPSQRNLTVLSVYFGYHVLFEIAESRRPGCPPEFMHIPVSADDPVFGSNSTNEKVLLPFQRAQWDHDTGKSPNNPRTQINHVTAWIDGSSIYGSSSSWCDALRSFQGGLLLAGSDDGIPKQSDSNYFMWSAPNPCNGQTGPEGLYEFGNSWANENIFTVAEGIIWFRYHNYLASQLQKEHPSWSDEELFQHARKRVIATFQNIAFYEWLPAYLAVSAPQYPGYQKYVDPGVSPEFLAAVVRFGLSLVPPGVYMRNRTCHYRSIMNSDGSRSPALRLCNSFWNRNNANLQSAEDVDDLLMGMASQIAEREDNIIVEDLRDYMYGTLQFSRSDAVALTIQRGRDFGLPSFNQVREALNMPPVQTWVEINPKLNQTNPQLFKDLAELYGNDISKLELFVGALLESDGKTGDLVTSIYLDQFERIRNADRFWFENKQNGLFTEEEIQTIRNTTYHDILLAVTSAQQGDIQENVFFWLNGDPCPQPQQVSESDLEPCTKAISISYR",
    "response#2": "YDGWYNNLAYHRRGTAGAPLMRFLPARYSDGAYQPLRGLPNARKISNAATRGDSALPSQRNLTVLSVYFGYHVLFEIAESRRPGCPPEFMHIPVSADDPVFGSNSTNEKVLLPFQRAQWDHDTGKSPNNPRTQINHVTAWIDGSSIYGSSSSWCDALRSFQGGLLLAGSDDGIPKQSDSNYFMWSAPNPCNGQTGPEGLYEFGNSWANENIFTVAEGIIWFRYHNYLASQLQKEHPSWSDEELFQHARKRVIATFQNIAFYEWLPAYLAVSAPQYPGYQKYVDPGVSPEFLAAVVRFGLSLVPPGVYMRNRTCHYRSIMNSDGSRSPALRLCNSFWNRNNANLQSAEDVDDLLMGMASQIAEREDNIIVEDLRDYMYGTLQFSRSDAVALTIQRGRDFGLPSFNQVREALNMPPVQTWVEINPKLNQTNPQLFKDLAELYGNDISKLELFVGALLESDGKTGDLVTSIYLDQFERIRNADRFWFENKQNGLFTEEEIQTIRNTTYHDILLAVTSAQQGDIQENVFFWLNGDPCPQPQQVSESDLEPCTKAISISYR",
    "response#3": "YDGWYNNLAYHRRGTAGAPLMRFLPARYSDGAYQPLRGLPNARKISNAATRGDSALPSQRNLTVLSVYFGYHVLFEIAESRRPGCPPEFMHIPVSADDPVFGSNSTNEKVLLPFQRAQWDHDTGKSPNNPRTQINHVTAWIDGSSIYGSSSSWCDALRSFQGGLLLAGSDDGIPKQSDSNYFMWSAPNPCNGQTGPEGLYEFGNSWANENIFTVAEGIIWFRYHNYLASQLQKEHPSWSDEELFQHARKRVIATFQNIAFYEWLPAYLAVSAPQYPGYQKYVDPGVSPEFLAAVVRFGLSLVPPGVYMRNRTCHYRSIMNSDGSRSPALRLCNSFWNRNNANLQSAEDVDDLLMGMASQIAEREDNIIVEDLRDYMYGTLQFSRSDAVALTIQRGRDFGLPSFNQVREALNMPPVQTWVEINPKLNQTNPQLFKDLAELYGNDISKLELFVGALLESDGKTGDLVTSIYLDQFERIRNADRFWFENKQNGLFTEEEIQTIRNTTYHDILLAVTSAQQGDIQENVFFWLNGDPCPQPQQVSESDLEPCTKAISISYL"
  },
  {
    "reference": "MAQNFSTVGDGKQMLLERDSSKRVLTGCFLSLLIFTTLLGNTLVCVAVTKFRHLRSKVTNFFVISLAISDLLVAILVMPWKAATEIMGFWPFGEFCNIWVAFDIMCSTASILNLCVISVDRYWAISSPFRYERKMTPKVACLMISVAWTLSVLISFIPVQLNWHKAQTASYVELNGTYAGDLPPDNCDSSLNRTYAISSSLISFYIPVAIMIVTYTRIYRIAQKQIRRISALERAAESAQNRHSSMGNSLSMESECSFKMSFKRETKVLKTLSVIMGVFVCCWLPFFILNCMVPFCEADDTTDFPCISSTTFDVFVWFGWANSSLNPIIYAFNADFRKAFSILLGCHRLCPGNSAIEIVSINNTGAPLSNPSCQYQPKSHIPKEGNHSSSYVIPHSILCQEEELQKKDGFGGEMEVGLVNNAMEKVSPAISGNFDSDAAVTLETINPITQNGQHKSMSC",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should contain a Helical; Name=1 membrane-spanning region. The designed protein sequence is ",
    "response#1": "RPVGISFVYLLMFFVSPFVPLATRRNFKGSVTAFFIILLTLSTLVLLGHITLQILAVSLTLPIYNCSFSERLLRHIGFVSFIDLQPFAIIEWLVPEVLVFATSLGSYLTVKRVASQPVGAEQLENGEVVDGQAENAQTSSQPSAADANGGDVQQATVTTPLQQQQQQLRKRVSMISQHIHFEGLVKISPLFCLATLFFAAVLRPSVPGGFYFLIFLLSGTYWATCQTLQRGFALLLRCVMVVLVLHSLSIVSYQTPWMQSHLNHTTLTARLIGLEPLIESYCSPDIRVFLYNNKLSLDSYLNPFALFFAYFALALTTKHLIKPRLVRQSTRKARTPQPLESGSSVAPSVTQRGNDMQLESMEQRSEQENTTTSILDQISYGFVSVGGFIYQNSYIFTNILMMAWSIVYHSWLTFVLLLSANVLWMIPNQRKAMMRSSPFIVLYAEALLIAQYIYGMDLNNEELPTSVPTAGINLQQIGFERPIENQMRPCVPLIVKTAFVLMFWVTSRQFFKEKRDRRRDSTLADFIAPLQITVGSAGSSYLINDGKKTSKFLKKAGDVIKNLLVRLWIWLLVLVIFLCAITGENMTGFRICYMALFLFFLLVFQSSSKAWVKIMYGFWLFLIFYAMSILILIYTYQFDKFDTYWSDYLNVSATLQKDIGLKRYQTKDLFLHLVSPTIIVILTVIQVHYFHKRFQ",
    "response#2": "GNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGISVVGKPFTIISCFCHRWVFGWFGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSYGVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASGGGQVFILSILFFCLLLPTAVIVFSYAKIIAKVKSSSKEVAHFDSRIHSSHVLEVKLTKVAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYTIFNLDFRRAFKKILCRG",
    "response#3": "GNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGISVVGKPFTIISCFCHRWVFGWFGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSYGVWLKRKHAYICLAVIWAYASFWTTMPLVGLGDYAPEPFGTSCTLDWWLAQASGGGQVFILSILFFCLLLPTAVIVFSYAKIIAKVKSSSKEVAHFDSRIHSSHVLEVKLTKVAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYTLFNVEFRKAFARIIR"
  },
  {
    "reference": "MSTNSTRTPLDHIRNFSIVAHIDHGKSTLADRLIQSTGGLAERDMSEQVLDSMDIERERGITIKAQTVRLHYKANNGETYVLNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGVEAQTLANVYQAIDNNHELVTVLNKIDLPAAEPERIKEQIEEVIGIDASDAVMISAKTGLGIPDVLEAIVNRLPPPKSDVGENGPLKALLVDSWYDTYLGVMVLVRVIDGVLTKGQQIRMMGSGAKYGVERVGVLTPKMVNVDSLGPGEIGFITASIKEVADTRVGDTITDDKRPTAQALPGFKPAQPVVFCGLFPVDAADFEDLRAAVGKLRLNDASFSFEMESSAALGFGFRCGFLGLLHLEIIQERLEREFNLDLVATAPSVVYEMTLTDGTEKELHNPADMPDVVKIKEIREPWIKATILTPDEYLGGILKLCQDRRGLQTELTYVGNRAMITYELPLNEVVFDFYDRLKSISKGYASFDYNIIDYREGDLVKMSILVNGDPVDALSMLVHRSAADRRGRGMCEKLKELIPPHMFQIPIQAAIGGKVIARETVRALRKDVTAKCYGGDATRKRKLLDKQKEGKKRMRQFGKVEIPQEAFIAALKMNDE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein have enhanced GTP + H2O = GDP + H(+) + phosphate.2. The designed protein have tr-type G domains with specific sequence motifs that are crucial for its activity.3. For general function, the protein need meet that Required for accurate and efficient protein synthesis under certain stress conditions; May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes; Binds to ribosomes in a GTP- dependent manner.; Back-translocation proceeds from a post-translocation (POST) complex to a pre- translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly4. The GTP binding site should be stable and able to withstand changes in environmental conditions.5. The designed protein should have guanosine tetraphosphate binding, translation elongation factor activity, GTPase activity, GTP binding, ribosome binding within plasma membrane to regulate positive regulation of translation. The designed protein sequence is ",
    "response#1": "NFSIIAHIDHGKSTLSDRIIQICGGLTERELKEQVLDSMDLERERGITIKAQSVTLDYKAEDGQVYQLNFIDTPGHVDFSYEVSRSLYACEGALLVVDAGQGVEAQSVANCYTAVEQGLEVLPVLNKMDLPQADPDRVAHEVEEIIGIDASDACRVSAKSGLGMEAKKIRNFSIIAHIDHGKSTLADRLIEYTGLISQREMQSQMLDNMDLERERGITIKLQTIRLVYKAKDGEEYYLNLIDTPGHVDFTYEVSRSLAACEGAVLIVDAAQGIEAQTLANVYLALEQDLEIIPVINKIDLPSARPDEIKTEIEDIIGLDASEAPLISAKEGLNIEDVLESIVKNVPKNIRNFSIIAHIDHGKSTIADRFIQICGGLSKREMAQQVLDSMDLERERGITIKAQSVSLNYCGRNGEVYHLNFIDTPGHVDFSYEVSRSLAACEGALLVVDASQGVEAQSVANCYTAIEQGLEVVPVLNKIDLPSAEPERVCQEIEEIIGLDASDALQVSAKTGQGIEELLAALVARVPPPQGDAEPIVQANILVPKEYLGNVITLCIEKRGVQKNMVYHGNQVALTYDIPAAEVVMDFFDRLKSTSRGYASLEYNFIRFEPAGMVTEPFMKAEITMPADNLNAVLKLAEQHKGTLIDLANSGDLIVASLKIPLSEIAYHFFSELKSVSHGFASLSTAFMSPISAAEMREPVVRASILVPQDFVGNVISECEQRRGTQLDMLFLGSQIQLTYELPMSEVVMDFFDRLKSISRGYASLEYNFERFEAAKLVRLEPIVQANILVPKEYLGNVITLCIEKRGVQKNMVYHGNQVALTYDIPAAEVVMDFFDRLKSTSRGYASLEYNFIRFEPADMVRLDVSDDIMEEPIVKTTIMVPNTYVGAVMELCQDRRGEMKDMQYIDDTRVNLYYEMPLNEVIYDFFDALKSKTKGYGSLDYELLGYRASDLVKLD",
    "response#2": "IRNFSIIAHIDHGKSTLSDRIIQICGGLTERELKEQVLDSMDLERERGITIKAQSVTLDYKAEDGQVYQLNFIDTPGHVDFSYEVSRSLYACEGALLVVDAGQGVEAQSVANCYTAVEQGLEVLPVLNKMDLPQADPDRVAHEVEEIIGIDASDACRVSAKSGLGMEALLERLVRDVLPEEEVLYAEPYIRATIITPTEVLGAVMTLCIEKRAYQTAVNYLDQKRVELVYEMPLAEILFGFYDRLKSISHGYASFDYELIDPVVNYKKIDSLSTIVHKTKILNQAKNVVEKMKNLIPRHQFDIIIQAVIGTKVIVRTTIKQLRKNVLAKCYGGDVTRKKKLLKKQKIGKKRMKQIGNITIPQEVFLKIAEE",
    "response#3": "IRNFSIIAHIDHGKSTLSDRIIQICGGLTERELKEQVLDSMDLERERGITIKAQSVTLDYKAEDGQVYQLNFIDTPGHVDFSYEVSRSLYACEGALLVVDAGQGVEAQSVANCYTAVEQGLEVLPVLNKMDLPQADPDRVAHEVEEIIGIDASDACRVSAKSGLGMEALLERLVRDVLASEAKLFEEPIAKCLILLPIKYLGNTLLLCSQRRGVQNDISYYNNQILLDYSIPMSEVVLNFFDQLKSVSSGYASLEYNFELYKVAPHMEEPMVKAEIMVTTEFVGAIMTLCQERRGIYLGMEYMESTRALLRYELPLNEIIYDFFDALKSRSRGYASFDYEMKGYVPSELVKLDGTTDYDGVFRINYAPVDALTFIVHREDAPNMTQQLVANLKTTVPRQLYPTPVQARVEGKVIARVDVPPLRKNAAVNGEQHSTSKKAALLRRQSANKRRASKNTIKLPQSVFNAIV"
  },
  {
    "reference": "MSYSQGPQQGPPRNNGGNRNGGNGPDKGNNPFFNFNQNGGDGKGPDSNRSFWRSPWLWAVLAVLIMMGIFGFFSSQNGPQTIDTKDGLELLAGNQRSGAVKSAEITEKMQVVELKLNDDFTKDVEGFQGKKTNKNFGKDVQFYYVFDQGPQVVKAVEKANLSEGYNSVVPQTSMMSLLISSVLPMLIILGVFWFLLSRMSSAAGGNMFGMGGKKNSARLNDGQTPKTKFADVAGEEAAVQEVEEIKDFLKDPSKYKALGARIPRGVLLYGPPGTGKTLLARAIAGEASVPFYSMAGSDFVEMFVGLGASRVRDLFDEAKKNAPAIIFIDEIDAVGGKRGSGMSGGHDEREQTLNQLLVEMDGFDNDTNLIIIAATNRPDILDSALLRPGRFDRQVAVEAPDLEGREAILRVHAKGKPFVPDVDLHTVAVRTPGFTGADLANVLNEAALLTARSDAQLIDNRAIDEAIDRVLSGPRRRTKGMALEEMRNTAYHEGGHAMVAAALHHTDPVTKVTILPRGRALGYTAVMPTQDRYSQSRNELLDQMAYAMGGRVAEEVVFHDPTTGASNDIEKATSIARQMVVEYGFSSKLGAIKWADGSDQDSGLDALKPRAYSEQTAEVIDREVLDLVETAHTEAWQIISRNRDVLDELVRQLLVKETLNKDELAAIFANLRKAPERELWLSDVNRPDSELPPVEIPKELERSVTGTTSPSGPASNTTPAQG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be involved in proteolysis by localizing to plasma membrane and exhibiting ATP binding, zinc ion binding, metalloendopeptidase activity.2. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation.3. The protein must exhibit the following characteristics: Plays a role in the quality control of integral membrane proteins..4. The protein contains novel AAA+ ATPase domains that confer a unique function or activity.5. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.6. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.7. The Zn(2+), ATP binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "LLVGPPGTGKTLLAKAVAGEADVPFFSMAGSDFVEMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAIGRARSKNGGFSGNDERENTLNQLLTEMDGFGTNSGVIILAATNRVDILDKALMRAGRFDRQITVIHMRDDVETGLQRAREVHPDAVIEDGLFKTLAWLQERASTG",
    "response#2": "MVMSEEEKTLTAYHEAGHAIVMLNVKGHEPLHKVTIIPRGRALGVTMFLPERDRLSQSRVQLDALVTSLFGGRVAEELIFGEENITTGASDDIRRATDICRRMVTEFGYSEKLGPLRYAVNQEEVFLGHSVTQQQNMSDATARLIDEEIRRLVEEGETRCRGILEEHLDALHALAKGLLEYETLSRDEIDRLAADAR",
    "response#3": "MKNIVLLVVVLLLGYSLVQLFLTNNTSSSNANVLEVPYSTFYDQVQKGNVATAVFQGQDVTGTFRNAISVTQSDGTTKTGTDYHFTQLPNGDQNLINALLNSNNNVGGGNNTYLV"
  },
  {
    "reference": "MPETKHNSLLGLDLQELAAIAQEAGEPRYRAQQLFDAVYGQRVKSAEQISTLPAEFRNVLTKQGLTVGLPVIEQKFVSRDGTVRYLIAFGDGQTVETVWMPEGDAGETGDGSEAGDELEKVSPKRVRATICVSSQVGCAVDCQFCMTALLGIKRNLSAGEIVGQICAVLNDQEVSPPQDRVNLVFMGMGEPFLNYENFTKAVRLLVEGVGIPESRMTVSTAGIVPRIYDFAEEPVRPKLAISLNASNDELRTRLMPLNKKWDLEKLMTAARDFPLRNRERLTFEYVLLDEVNDSVQNAREVVELIRGIRAKVNLIALNSGPEIGFRTPSEERVLAFQKVLVAANIPTFIRRPRGRDIYAACGQLKRTVEISA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must be engineered to have a cooperative binding mechanism with [4Fe-4S] cluster to ensure proper regulation. The designed protein sequence is ",
    "response#1": "LTPQHYAYLKISEGCNHRCSFCIIPSMRGDLVSRSIGDVMTEAENLVNAGVAELLVISQDTSAYGVDVKYRTGFWNGRPVKTRMTELARALGSLGVWVRMHYVYPYPHVDEIIPLMADGLILPYLDVPLQHASPRILKAMKRPASSENNLARINAWREICPDITIRSTFIVGFPGETDEDFECLLEFLQEAQLDRVGCFAYSAVDGAAANALDNPVPEPLKQERLARFMEVQESISAV",
    "response#2": "FQSYVPIMNGCNNFCTYCIVPYVRGREVSRPVNEILQEITELSSRGVREITLLGQNVNSYKGEDGEGRLIDFPKLLTLIAREADKTDMIRWIRFMSSHPKDMSDALIDTIAAEKRLCKLVHLPVQHGSDTILKRMNRVYTIEHYKNRIKRLKETIPDIALSTDILMGFPGETEDDVKATLDLMQEIEFDSAFMYHYNPREGTKAFNYPDRIPEEVKIERLGRVIRRKIGRIETVLIDDIDGDQAIGRTAADAPEIDGVVYLSGADGLQPGDLVEAQIVNSDGHDLWAAPPV",
    "response#3": "KISEGCNHRCSFCIIPSMRGDLVSRSIGDVMTEAENLVNAGVAELLVISQDTSAYGVDVKYRTGFWNGRPVKTRMTELARALGSLGVWVRMHYVYPYPHVDEIIPLMADGLILPYLDVPLQHASPRILKAMKRPASSENNLARINAWREICPDITIRSTFIVGFPGETDEDFECLLEFN"
  },
  {
    "reference": "MPTFFIDAFIFLAKLFCAMEYFPVFLDLKQRRCLLVGGGDVATRKGRLLAKAGAVLRVVAPEVSAELRDLVAQQQGELRECEYQSSDLDHCVLVVAATDDEALNAQVSADAKARNLPVNVVDSPALCTYITPAIIDRSPLVIAVSSGGDAPVLARLIRAKLETLIPANYGKLAQLASRWRDRVKARFSDTEGRRRFWEKILQGPAAELTLNGQTDAAETLMAEEIQCDDVTITRGEVYLVGGGPGDPELLTLRALRLMQQADVVLYDRLVSDAVMELVRRDAERIYVGKRRSEHSMEQDNINQLLVDLALQGKRVLRLKGGDPFIFGRGGEEIELLAQHQVPFQVVPGITAASGCAAYAGIPLTHRDYAQSVRFVTGHLKSDNANLQWPELAMPGQTLVFYMGLVGLRDICETLVSHGRAATTPVALVEKGTTPQQRVLVADLASIADLVDAQEVHAPTLVIVGEVVLLHRQLHWFNPSP",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must have at least one Sirohaem synthase dimerisation domain. The designed protein sequence is ",
    "response#1": "VGAGPGDPDLLTFRALRLMQKADVVVYDNLVAKAIVDLTRRDAQRIFVGKQRAKQSMRQEEINELLVRLAREGRRVVRLKGGDPFIFGRGGEEIERLAACGIPFQVVPGITAATGVASYAGIPLTHRDHAQSCLFVTGHLRDGTLNLDWDALARPDQTVVVYMGLHGLDTLCGELVRHGMPDGTPIAIVQQGTTQNQRVFIGTLATLPAIAXRAKPQAPTLIIVGGVTG",
    "response#2": "VGAGPGDPELLTIKAMQCMQQADVIVHDGLVSADVLALARRDAERISVAKQAGFHSLPQDEINALIVRLAQAGKRVCRLKGGDPFIYGRGGEECEALVAAGIRYSVVPGVTAAAGCAAYAGIPLTHRDYAQTVQLVTGHCRANGDEPDWPSLAKARQTLVVYMGLINSATLSAQLIAHGRSSDTPVAIIENGTRPEQRSITGTLAQLPQLIAEHQVQSPALIIIGEVL",
    "response#3": "GEVWLVGAGPGDPELLTIKAMQCMQQADVIVHDGLVSADVLALARRDAERISVAKQAGFHSLPQDEINALIVRLAQAGKRVCRLKGGDPFIYGRGGEECEALVAAGIRYSVVPGVTAAAGCAAYAGIPLTHRDYAQTVQLVTGHCRANGDEPDWPSLAKARQTLVVYMGLINSATLSAQLIAHGRSSDTPVAIIENGTRPEQRSITGTLAQLPQLIAEHQVQSPALIIIGEVVARLSP"
  },
  {
    "reference": "MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The intended protein structure should align with helix-turn-helix-helix-strand-strand-turn-strand-turn-strand-turn-strand-helix-helix-helix criteria.2. Design the protein with a signal peptide incorporated.3. The protein should be optimized for stability and solubility when expressed in outer membrane-bounded periplasmic space where it will be involved in chaperone-mediated protein folding, regulation of catalytic activity. The designed protein sequence is ",
    "response#1": "VGGAEELPRRVGCQLLHVAGRHHPDVFAEFFSARRVLRLLQGGAGPPGPRALACVQLGLQLLPEGPAADEVFALLRREVLRTVCERPGPAACAQVARLLARHPRCVPDGPHRLLFCQQLVRCLGRFRCPAEGEEGAVEFLEQAQQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVVQHLPLELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWIIALLKGLAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTFQHSHEAFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVHCQ",
    "response#2": "VGGAEELPRRVGCQLLHVAGRHHPDVFAEFFSARRVLRLLQGGAGPPGPRALACVQLGLQLLPEGPAADEVFALLRREVLRTVCERPGPAACAQVARLLARHPRCVPDGPHRLLFCQQLVRCLGRFRCPAEGEEGAVEFLEQAQQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVVQHLPLELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWIIALLKGLAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTFQHSHEAFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVHCR",
    "response#3": "VGGAEELPRRVGCQLLHVAGRHHPDVFAEFFSARRVLRLLQGGAGPPGPRALACVQLGLQLLPEGPAADEVFALLRREVLRTVCERPGPAACAQVARLLARHPRCVPDGPHRLLFCQQLVRCLGRFRCPAEGEEGAVEFLEQAQQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVVQHLPLELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWIIALLKGLAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTFQHSHEAFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVHCSQ"
  },
  {
    "reference": "MAPDSPPPPPPPPPSPNRRGPGANRIDPGWPRWALWLALGTLTLIVVISLTAPGNNGDTITYNQFLDAVAAGDVESVEINTENGKVVGVFDDGEKFNTVGPDELSADDIDARFGDRVEVTLVRPEPGLLAQWLPLILFFGLFFLLMIYLQRRAQGQMSGIMNVGKSKAKTHSSEMPGTTFDDVAGYAGVKQEITEVVDFLRRPEKYGSIGARTPKGVLLVGPPGTGKTLIARAVAGEAGVPFLSVTGSDFMEMFVGVGASRVRDLFETARKHGRAIIFVDEIDSIGRKRGAGLGGGHDEREQTLNQMLSEMDGFEASEGIVMMAATNRPDILDSALLRPGRFDRQVVVPLPELEDRRAILSVHIKGKKIAEDVDLDVVSRGSPGMSGADLANLVNEAALFAVREGADHVFMRHFEQARDRIIIGQKRDSMVMSDQEKELTAYHEAGHAVCAVVQPNADPLHKVTIIPTGMALGVTMQLPAEDRHGYQQDYLRDRLVVMMGGRVSEQLVYDQVSTGAKGDLVSATQMAHAMVSEWGMSDQVGPMAWGNNEQVFLGDGLMASREYSDEMAHVIDEETQRILREEEERCRQTLTRHRKALDLVARSLLEHETISGEEVERLIKVADEVPPLADPTTVPAPPPPPPDMATHPDPRDR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should exhibit Pro residues compositional bias.2. The designed protein should have ATP binding within plasma membrane to regulate protein catabolic process.3. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins; Plays a role in the quality control of integral membrane proteins.4. The designed protein must have at least one AAA+ ATPase domain.5. The Zn(2+), ATP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "VLLVGPPGTGKTLLAKAVAGEANVPFFSMSGSDFVEMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAIGRARGRNVNFSANDERENTLNQLLTEMDGFASNQGVIILAATNRADILDRALMRAGRFDRQIHVLTGLTPQQLADRAVTEAFWVVLMLLVLALFNLFSGSGNNLQSNEITYTEFVNAVENGQVRNVTLDGEQVRFRKSDGGDFVAIKPEDAEITTLLLDNNVPVQARPG",
    "response#2": "LLEGPPGTGKTLLARAIAGEAGVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIVFIDEIDAVGRHRGAGYGGGNDEREQTLNQLLVEMDGFEANEGVIIIAATNRKDVLDPALLRPGRFDRQITVHLPYLEETHTVGIHALYQRLGMADVPDADLNALSKSTPGMTGADLANLLNEAALAAARQGESQITQRDLLRAAHALNPQTPEGYEERALRHVAELLGH",
    "response#3": "VLLMGPPGTGKTLMAKAVAGEAEVPFFSISGSQFVEMFVGVGASRVRDMFNDAKKEAPAIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQILAEMDGFESDESIVVMAATNRPDVLDSALLRPGRFDRKVTLHVPYLEPSDAINHG"
  },
  {
    "reference": "MRPIFTHQKILILISGSIAAYKILDCISQLRKYGAHIKVVMSSEACKFVQPLSFEALSGNVVLCEHNQSWSNDVNHIGYAKWADVVLVAPASANTIAKIAIGAADNVLLATLLASQAKKIIAPAMNTTMLYAPQTQENLHKLAGLGYEIIPPRAGLLACGDEGEGALANVQEIIFRLGRILHTKTRSLWHNRAVIVTGGGSKESIDCVRYISNYSSGKQASYLAIALYMLGAQVKLIASSALELPLGVEYVQVQSVQDYARATNEALNVRLDSINAKNADSKTSKTRPKASHSLDKAHKHIVFMAAALADYAPRFYNGKLKKQQVGDVLKLQCFKTPDVLDSIPAHSAFKIGFKAESDEASAQDYARDMLAKKHCDMVCLNVIGADNPFGSEENHIQIITQNNIQAVSGSKFDVSLAIASAFERFYDEL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A2. The design of the protein must focus on the precise arrangement of amino acids in the Proton donor to enable effective catalysis of H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.3. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.4. The designed protein should be able to compete with endogenous proteins or inhibitors for CTP binding.5. The presence of DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.6. A protein with metal ion binding, phosphopantothenate--cysteine ligase activity, FMN binding is needed to enhance coenzyme A biosynthetic process, pantothenate catabolic process in a specific context.7. A protein should be able to manipulate the activity of enzymes in the Cofactor biosynthesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "MNIILGVTGGIAAYKSILLLRQLTDAGYSVQVVMTEAAQQFVAPLTFQALSGRAVRSELFDAAAENAMDHIALARWADAIVVVPASADFIARLRAGMANDLLTTLCLATEAPILLAPAMNRVMWANPATQENVQVLQQRGVHIIAPESGPQACGEVGEGRMSEPAAIFSTDAEGKLSGIVEEKDASEEQKKIQEVNSGIYAFDLKALTGALKQLSTDNAQGELYLTDVLGIARDDGLSVASHVTGDHREILGVNDRVQLAEAGRELNRRTVERWMREA",
    "response#2": "MNIILGVTGGIAAYKSILLLRQLTDAGYSVQVVMTEAAQQFVAPLTFQALSGRAVRSELFDAAAENAMDHIALARWADAIVVVPASADFIARLRAGMANDLLTTLCLATEAPILLAPAMNRVMWANPATQENVQVLQQRGVHIIAPESGPQACGEVGEGRMSEPAAIFSEPADPGKLFAAVEAILTGAEPLTGKKVTITAGPTVEAIDPVRFLSNHSTGKMGFALARACRDAGAEVTLIAGGKVDLTTPLDVQRVNVLSANDMLQAASQSAAAGCDIFIATAAVADYRTRDAAPQKIKKTQEEMTLDLVKNPDILATISHTYPDMFVVGFAAETQDVEKYARGKLASKDLDMIACNDVSRKDIGFASDENAMTVFWTGGAEKNNQALVIGGATKEYIDDVRFISNGSSGKMARYLTMGLLSLGAKADFLDVSNMDVERAYQKILEIFDRYDIVVMNAAISDYKVSSKHNGKIKKTQDKIVLELLKTKDILKELGIRKKPYQKLIGFALEEKEHLIENAKSKLINKNLDAVVANPISVMESLVDFILHGKKVIITAGATIEAIDPVRFLSNHSTGKMGFAIARACVNAGADVTLVAGRAVHLPTPFGAKRLTVGNANDMLTACQSVMTNDSDTIFIATAAVADYKVKSIATQKIKKTSDQDGLTLELIKNPDILATMSHTFPSAIMVGFAAETQDAENYARTKLTAKNLDMIAVNDVSDKSIGFGSDNNAMTVFWQGGAETLDLDSKTR",
    "response#3": "MNIILGVTGGIAAYKSILLLRQLTDAGYSVQVVMTEAAQQFVAPLTFQALSGRAVRSELFDAAAENAMDHIALARWADAIVVVPASADFIARLRAGMANDLLTTLCLATEAPILLAPAMNRVMWANPATQENVQVLQQRGVHIIAPESGPQACGEVGEGRMSEPAAIFSEIAADPIGTGRLVGKRVLMTAGPTFEAVDPVRFIGNRSSGKMGFALAEALAQAGAQVTLIAGPVSLPTPPGVERIDAESAEQMLEAVLARLSDTDMFISTAAVADWRVAKPAEQKLKKQSGQDSMTLELVRNPDIVATVAQHPERPRWVVGFAAETDNLLEHAREKLQQKGLDMIVANQVGDGRAFGQADNEAWLVTAAGVEDWPRMGKDEVAFRLAERIAEALEED"
  },
  {
    "reference": "MSLEKSLDEIINERTNGFDHKHSRRRGSQNRISKKSRLTYKFKRASKEHNSSPDDGPWQHDLDQEQDAHPRTTHLQKRQHSENRFGVRVENLHYQVLEKDVLSLFENFHPIRVIMNYDRAGRSEGSCDVYFETSQDAEDAQKTLQSTNLKGSEIQISKKSPPSLFDRISDMPHSARKPSRSSRSNRGFNRSSKKDDRSFRSSSKKSSNNSISHEDLDKELDEYAMSFHAASTVSSHSSQDFTPSIANAHEKNEPVAPSKDSNLTEEMDLQMEAV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that can perform mRNA binding in nucleolus to support regulation of transcription elongation by RNA polymerase II.2. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "DPTVANRLDSQYIQTASDLRNYRDGTEIIAYASGKTGSVLNIAVLTRQNTLHLNRHNNVTSIELHSPIKSIKIPGASESIGRRSNLVGIITENSFQIFRIESVHSRSCDVMVSSSEPLYFVEIDDLQVVDFAFNPWDLQQFAIIDIKGNWSIGRIPKNFNNNNKRKLQLIDNLHGTIFDPEELSSWKRIEWFSHFQKILVFDRSKMIEIDFMNNWQTEVVQAKAWSNIRDYKRIDDKNGILLTSREIIIVGASESNDPVRRISWKHDLDPDDTTLRITVQKVKKPDHILLVAFVYSMRHKRIYMHVFSHRKANLFQSLGCSTVLEIC",
    "response#2": "DPTVANRLDSQYIQTASDLRNYRDGTEIIAYASGKTGSVLNIAVLTRQNTLHLNRHNNVTSIELHSPIKSIKIPGASESIGRRSNLVGIITENSFQIFRIESVHSRSCDVMVSSSEPLYFVEIDDLQVVDFAFNPWDLQQFAIIDIKGNWSIGRIPKNFNNNNKRKLQLIDNLHGTIFDPEELSSWKRIEWFSHFQKILVFDRSKMIEIDFMNNWQTEVVQAKAWSNIRDYKRIDDKNGILLTSREIIIVGASESNDPVRRISWKHDLDPDDTTLRITVQKVKKPDHILLVAFVYSMRHKRIYMHVFSHRKANLFQSLGCSTVLEIC",
    "response#3": "DPTVANRLDSQYIQTASDLRNYRDGTEIIAYASGKTGSVLNIAVLTRQNTLHLNRHNNVTSIELHSPIKSIKIPGASESIGRRSNLVGIITENSFQIFRIESVHSRSCDVMVSSSEPLYFVEIDDLQVVDFAFNPWDLQQFAIIDIKGNWSIGRIPKNFNNNNKRKLQLIDNLHGTIFDPEELSSWKRIEWFSHFQKILVFDRSKMIEIDFMNNWQTEVVQAKAWSNIRDYKRIDDKNGILLTSREIIIVGASESNDPVRRISWKHDLDPDDTTLRITVQKVKKPDHILLVAFVYSMRHKRIYMHVFSHRKANLFQSLGCSTVLEIC"
  },
  {
    "reference": "MSRLVLASHNAKKLQELQRILGGQGIEVVGLNTLTDAEPPAETGSTFEQNALIKARAASRETGLPALADDSGISVDVLNGMPGVRSARWYGPDATDVDNVELLLRQLADVEPERRGAHFVCALAFVRPNEDGEVAEDVVLGEVSGRLTEQPSGRHGFGYDPIFVGDGHTMTMAELTPAEKDQISHRGRALELMAPLVVADLTRE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.2. The designed protein must possess ITP diphosphatase activity, dITP diphosphatase activity to support nucleotide metabolic process, purine nucleoside triphosphate catabolic process under certain conditions. The designed protein sequence is ",
    "response#1": "SEYIEGTSLNLISNEKYPFEDVAMLLKKIHNVEWKSDNDYNPFKRLEKYEQYCIEKGFVLPKSYLNIRDKFFTYKDYLQSLPKSFAHGDSQPSNFIITDQGLKVVDFEFTGNIDHIYDIACFANMRISDGEQLLKAAG",
    "response#2": "SEYIEGTSLNLISNEKYPFEDVAMLLKKIHNVEWKSDNDYNPFKRLEKYEQYCIEKGFVLPKSYLNIRDKFFTYKDYLQSLPKSFAHGDSQPSNFIITDQGLKVVDFEFTGNIDHIYDIACFANMRISDGEQLLKALE",
    "response#3": "MDKKLVLATRNQGKTYEIRQMLGDLPFEIVGLSDFPDVPEVVEDGATFAENALKKARTVARETGLITLADDSGLEVDALGGAPGVRSARFAGEEATDEANNAKLLTELDGVPLEERAARFVCVIAVAFPDGRVETVRGETPGRILTEPRGSGGFGYDPHFFVPELGRTFAEMSLAEKNRLSHRGRALQALREVLAELR"
  },
  {
    "reference": "MKLFLATGNKHKIEEIKAIFHENEVEIFSILDGISIPEVVEDGKTFEENSQKKALEIAKYLNMMTVADDSGLCVDALGGAPGVYSARYSEEGTDEANNQKLIQNLKGIDNRKARFVSVISFAKPDGEVFSFRGEVEGEIVDDRRGEFGFGYDPYFYVKEYGKTLAEMPEVKNQISHRANALKKFQEFWRQKKSF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.3. The designed protein should have metal ion binding, ITP diphosphatase activity, XTP diphosphatase activity, nucleotide binding, dITP diphosphatase activity to facilitate purine nucleoside triphosphate catabolic process, nucleotide metabolic process.4. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in dITP + H2O = dIMP + diphosphate + H(+) are properly positioned for optimal catalysis.5. The designed protein should have a binding site that is specific to the target Mg(2+), substrate ligand. The designed protein sequence is ",
    "response#1": "MINPNDTVYIATTNPGKVKEYRDLLAPLGVTLVSQKEAGFPEVIEDGKTFEENAYKKAVTYAEATGQVTLADDSGLKVDALGGAPGIYSARYAGEGATDADNNAKLIRELADVPVEERGASFVCVIAVAMPDGTIFTTCGWMHGYIAREPRGNNGFGYDPIFYVPELNKTAAQLSAEEKNAISHRGKALAELLPKLKELLSQ",
    "response#2": "MKRIVFATGNQGKVREIRDILAPLQVELIPQSQLNIPEAAETGTTFVENAIIKARHAAKATGLPAIADDSGLVVDALGGAPGIFSARFAGEPKSDARNNAKLLHELAGIPAAQRQARFFCTLVLLQSAEDPTPIIAEGEWHGEILDTPRGSGGFGYDPLFLVPEKGKTAAELTSDEKNQVSHRGLALRQMVARLKAIA",
    "response#3": "MKLVFATNNENKIRELQDILGDDFEIKALKDIGCLEDIPETADTIEGNAILKAEYVTEKYGYDCFADDTGLEVEALNNEPGVYSARYAGEQRNADDNMDKVLTELEGKTDRSARFRTVIALILDKKEYLFEGICKGKITAQQKGSEGFGYDPVFVPEGYDQTFAEMELEVKKSISHRGKAVQKLVTFLNSK"
  },
  {
    "reference": "MPFIKAVKSSPYFSRYQTKYRRRREGKTDYYARKRLIAQAKNKYNAPKYRLVVRFSNRFVTCQIVSSRVNGDYVLAHAHSSELPRYGIKWGLANWTAAYATGLLVARRALAKVGLADKYEGVTEPEGEFELTEAIEDGPRPFKVFLDVGLKRTSTGSRVFGAMKGASDGGLFIPHSPNRFPGFDIETEELDDETLRKYIYGGHVAEYMEMLIDDDEERYQKQFSGLIADGIESDQLEDIYAEAYAKIREDPSFQKSGKDAAAFKAESLKYTQRKLTAEERKERFNAKVIEAGRA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.; The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain; The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules; Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.2. The ideal protein would be one that plays a critical role in cytoplasmic translation, ribosomal large subunit assembly through structural constituent of ribosome, 5S rRNA binding within cytosol, cytosolic large ribosomal subunit. The designed protein sequence is ",
    "response#1": "MSQIAAHVTVRGEKAAEILEKGLKVREFELRARNFSTQGSFGFGIQEHIDLGIKYDPSIGIYGMDFFVVLSRPGFRVAHKKP",
    "response#2": "MKISVHVTVRGPKAEEILERGLKVKEYELRKRNFSETGNFGFGISEHIDLGIKYDPGIGIYGMDFYCCMTRPGERVSREPRSGDSGGGRLEDIYTSAHEAIRADPAFKPTEKKFTKEQYAAESKKYRQTKLSKEERAARVAAKIAALS",
    "response#3": "LEDIYTSAHEAIRADPAFKPTEKKFTKEQYAAESKKYRQTKLSKEERAARVAAKIAALS"
  },
  {
    "reference": "MLTNRQLQILQVIIDDFILSAQPVGSRQISKKQGITFSPATIRNEMADLEELGYLEKTHTSSGRVPSEKGYRFYVDHLLSPQGINSRDIQQIQSIFNDRLVEVEHIIRKSANILSELTSYTSILLGPDVQRHRVKRFSIVPLSSDTAVAIIVTNNGHVENRLFNLPPDFTASDLEKMVNILNDRLIGVSLEELHKSLEAEVLAVLQQHVQSADDFYPRTRNSDNAIIQKAKIFYGGKTNMFNQPEFHDLNKVRMILDLMETTSQVQSLFHPNESGIHIRIGSENKQLEMENCSVITTTYSIGDDQQGAIAIIGPTRMDYKRVVALLDVIRLDLTQAFTKNRSE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Negative regulator of class I heat shock genes (grpE-dnaK- dnaJ and groELS operons)2. The designed protein should be optimized for DNA binding activity in order to improve negative regulation of DNA-templated transcription. The designed protein sequence is ",
    "response#1": "LVNATTTLSGLSACAGLVLVPKQELVLKQLGFVVLDDNRALAIIVGSDGSVENRVIELSSGFPASALTEASNYINAHFSGYSFSEAKKRLFSQIDLERSELDSAASDLIKRGLAVWSEDSRKRPVLIVRGQSHLLQDASEDLDRAKQLLEELEDKKEIAGLLEKVSESDAAQIFIGSENKLFSLSGSSVIASPYHGEDGHMVGVVAVIGPTRLNYGRIF",
    "response#2": "LVNATTTLSGLSACAGLVLVPKQELVLKQLGFVVLDDNRALAIIVGSDGSVENRVIELSSGFPASALTEASNYINAHFSGYSFSEAKKRLFSQIDLERSELDSAASDLIKRGLAVWSEDSRKRPVLIVRGQSHLLQDASEDLDRAKQLLEELEDKKEIAGLLEKVSESDAAQIFIGSENKLFSLSGSSVIASPYHGEDGHMVGVVAVIGPTRLNYGRIQ",
    "response#3": "LVNATTTLSGLSACAGLVLVPKQELVLKQLGFVVLDDNRALAIIVGSDGSVENRVIELSSGFPASALTEASNYINAHFSGYSFSEAKKRLFSQIDLERSELDSAASDLIKRGLAVWSEDSRKRPVLIVRGQSHLLQDASEDLDRAKQLLEELEDKKEIAGLLEKVSESDAAQIFIGSENKLFSLSGSSVIASPYHGEDGHMVGVVAVIGPTRLNYGRIR"
  },
  {
    "reference": "MARILGIDPGSRITGYGLIETNNKKTVYIAAGCIRAGEGGLAERLGQIFQGITGIIQAYHPDEVAVEQVFMHQNPGSALKLGQARGAAICAAVNAVLPIFEYTPSQVKQAVVGRGDAAKSQVQYMIRLLLKLPAEPATDAADALACALCHEHAGPLLAGLAGQVRGRRRGRYYR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must have high specificity towards nucleic acid binding, crossover junction endodeoxyribonuclease activity, magnesium ion binding.2. The protein must exhibit the following characteristics: Endonuclease that resolves HJ intermediates; Cleaves cruciform DNA by making single- stranded nicks across the HJ at symmetrical positions within the homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction; The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair; HJ branch migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.. The designed protein sequence is ",
    "response#1": "MIAYLEGYIAEFGETSCVVATSSGIGYEVFLTTPTRSTLPNKGEQVRFYICHIVREDIQELFG",
    "response#2": "MITSIQGTLVSATPLQAIVEVAGFGYEVHIPVTTAERLPAAGAAVKLHTLVIYREDSQTLYV",
    "response#3": "MITSIQGTLVSATPLQAIVEVAGFGYEVHIPVTTAERLPAAGAAVKLHTLVIYREDSQTLYG"
  },
  {
    "reference": "MNEGNSAGGGHEGLSPAPPAVPDRVTPHSTEISVAPANSTSTTVRAAGSVGAALPATRHHQHIATQVKGIASSSSKQQKQLASAQLPVPLSPLPQQQQQTAEATAAAAAPAHSNVSVSSSTIEASVLPPQAKRQRLDDNEDRTSAASIVGPAESSNIVSSLLPASVASSSEVGGLSSTALQDLNALKKRILQQKLQILRNLKERHLENVSEYFYLQNGGSMMDYPAWRKKTPTPQFISYSNANRIDQLIHEDKPSTSAAAAAAQNQKYTTQQTDSVESSLVSGIGTGATKGAPLDGNISNSTVKTNTQSQVPSKIGSFTESTPAATESNSSTTVPGTATSGAATSTSATSAEASGNVLAVEAEIKIPAVGATPVAISTKLPAAVVQLTQQGGTPLLPCNTSAGSTALRRPQGQNNASSGSAAASGGGGSLTPTPLYTGNGPAALGGSGGLTPGTPTSGSLLSPALGGGSGTPNSAAQEFSFKAKQEVYVMQRISELQREGLWTERRLPKLQEPSRPKAHWDYLLEEMVWLAADFAQERKWKKNAAKKCAKMVQKYFQDKATAAQRAEKAQELQLKRVASFIAREVKSFWSNVEKLVEYKHQTKIEEKRKQALDQHLSFIVDQTEKFSQQLVEGMNKSVADTPSLNSSRLTSPKRESDDDFRPESGSEDDEETIAKAEEDAADVKEEVTALAKESEMDFDDFLNDLPPGYLENRDKLMKEEQSSAIKTETPDDSDDSEFEAKEASDDDENTISKQEEAEQEIDHKKEID",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in negative regulation of gene expression, potentially with improved properties such as stability, specificity or selectivity.2. The protein must exhibit the following characteristics: Required for somatic stem cell self-renewal but not for germline stem cell self-renewal.3. The designed protein have Helicase C-terminal domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "IGSKPWSLEISWALRVAQHQSFAMIAPTGVGKTTFGLVMTLFLSYKYGKKVYIIVPTTVLVEEYEKRLWQFSEKLGITLKIIAASSKIHGKRKIELEESIASGSFDILITTSAFLQRNFNDVFKKMLSNNIKMDLIFVDDVDAIIKGSKAIDMVLQLLGFSEEDISNGYKMIDLRRRLLQCENARESSRCYIDGKPIDVIYEELQKKVLEKRERCGILIVASATGRARGKRIKLFRELLGFSIGTAIEVT",
    "response#2": "FGSLRGHVVGLRYYTGVVNNNEMVALQRDPNNPYDKNAIKVNNVNGNQVGHLKKELAGALAYIMDNKLAQIEGVVPFGANNAFTMPLHMTFWGKV",
    "response#3": "IGSKPWSLEISWALRVAQHQSFAMIAPTGVGKTTFGLVMTLFLSYKYGKKVYIIVPTTVLVEEYEKRLWQFSEKLGITLKIIAASSKIHGKRKIELEESIASGSFDILITTSAFLQRNFNDVFKKMLSNNIKMDLIFVDDVDAIIKGSKAIDMVLQLLGFSEEDISNGYKMIDLRRRLLQCENARESSRCYIDGKPIDVIYEELQKKVLEKRERCGILIVASATGRARGKRIKLFRELLGFSIGTAIEVP"
  },
  {
    "reference": "MILQDILYKVAISSVKGSTAIEVHNLQIDSRKISKGDCFIAVQGTAADGHLFIDKAIDNGAAIVVCEVLPATLKDTITYIQVDNSAAASGLMSHNFYGEPSAKIKLVGVTGTNGKTTIATLLFKLFSALDYKCGLISTVQNQIAATVIPATHTTPDVISLNALLKQMADEGCDYVFMEVSSHAIHQHRIAGLQFAGALFSNITHDHLDYHKTFDEYIRVKKSWFDNLSATAFAISNADDKRGAVMLQNTVAKKYFYSLKTMPDFKGKILDNSLTGLHLTVNDVEVHFRLIGEFNAYNLLAVYGAAICLGEEKNEVLQILSSLDGAEGRFDYVISNQERLIGIVDYAHTPDALLNVLATIKKLRQGNEQVITVVGCGGDRDKTKRPVMAEVACEYSDKVILTSDNPRSEDPMEILKDMEAGVNVAARKKVISIADRREAIKTAVSLAQKEDIVLIAGKGHEKYQDIKGVKYDFDDKKVLNEMFELLEK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Mg(2+) is required for the proper folding and stability of the protein.2. The protein design must feature the Meso-diaminopimelate recognition motif motif.3. The protein design must be able to perform magnesium ion binding in order to contribute to cell cycle, regulation of cell shape, cell wall organization.4. The protein contains novel Mur ligase C-terminal domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "ITGTNGKTTTVTLLFRLFRKLGYRCGLISTVQNQIDDTVIPSTHTTPDSINLNKLLAEMVEAGCTYAFMEVSSHSVVQHRITGLSFTGAIFSNITHDHLDFHKTFDNYIKAKKGFFDMLPKTAFALVNIDDRRGSVMVQNTKASINTYSLETLATVKGRIVSDSIYGLEMEIDNKEVHFQLIGTFNAYNLMAIYGARLGLKPEEWVKAA",
    "response#2": "VTGTNGKTSTAWWLAQALSRLGRKCGVVGTLGIGEPGAMVATGLTTPDPVLLQQQLRRFVDEGFAACALEASSIGLVERRLDATAIQVAIFTNFTQDHLDYHPSMQAYWEAKERLFGWPGLKAAVINVEDPKGLELAASLTAKGLDVWTFSCTGSARLQASDIQHGAHSLRFDVVEGAERHSISTQLVGQYNVSNLLGVMAITGTNGKTTTVTLLFRLFRKLGYRCGLISTVQNQIDDTVIPSTHTTPDSINLNKLLAEMVEAGCTYAFMEVSSHSVVQHRITGLSFTGAIFSNITHDHLDFHKTFDNYIKAKKGFFDMLPKTAFALVNIDDRRGSVMVQNTKASINTYSLETLATVKGRIVSDSIYGLEMEIDNKEVHFQLIGTFNAYNLMAIYGVVSGL",
    "response#3": "ITGTNGKTTTVTLLFRLFRKLGYRCGLISTVQNQIDDTVIPSTHTTPDSINLNKLLAEMVEAGCTYAFMEVSSHSVVQHRITGLSFTGAIFSNITHDHLDFHKTFDNYIKAKKGFFDMLPKTAFALVNIDDRRGSVMVQNTKASINTYSLETLATVKGRIVSDSIYGLEMEIDNKEVHFQLIGTFNAYNLMAIYGLGRKVGLEPSRIEITGTNGKTSVTSFTRQLWELLGEKSASIGTLGVQGKDLGKNELHNLSFSTPEPLVLYPLLEQLAHNGINHLAMEVSSHGLDMYRAEHVHFKVAAFTNLSHEHLDYHKTMEAYFQAKVRLFKEVLSLDGTAVVNTNTVFGQEIAKVCAQRGLRLVTLGREKSDIYVKNIVTEARGSRIAFQAFGKNYTIFLPLIGQFQVENVLTAFATSVALDESFEQIEAGISSFNGVKGRMQQLGGQEAPLMVIDYAHTPDALEKTITTLRQVAQQRGGQLWCVFGCGGDRDPGKRPQMGTIAQAADHVIVTSDNPRSEEPSAILSQIVAGIDAQKMQKDALHVIEDRAAAILWAGRHAAKNDVVLLAGKGHETYQIIGEKKLDFDDRETASEALLELES"
  },
  {
    "reference": "MSTNPKPQRKTKRNTNRRPQDVKFPGGGQIVGGVYLLPRRGPRLGVRAARKTSERSQPRGRRQPIPKARRPEGRTWAQPGYPWPLYGNEGCGWAGWLLSPRGSRPSWGPTDPRRRSRNLGKVIDTLTCGFADLMGYIPLVGAPLGGAARALAHGVRVLEDGVNYATGNLPGCSFSTFLLALLSCLTVPASAYQVRNSSGLYHVTNDCPNSSIVYETADTILHSPGCVPCVREGDVSKCWVAVAPTVATRDGRLPTTQLRRHIDLLVGGATLCSALYVGDLCGSVFLVGQLFTFSPRRHWTTQDCNCSIYPGHITGHRMAWDMMMNWSPTTALVVAQVLRVPQAILDMIAGAHWGVLAGIAYFSMAGNWAKVLVVLLLFAGVDAATYTTGGSVAHSTATLTSLFRQGPKQDIQLINTNGSWHINRTALNCNASLDTGWVAGLFYYHKFNSSGCPERMASCRPLADFDQGWGPISYANGSGPEHRPYCWHYPPKPCGIVPAQNVCGPVYCFTPSPVVVGTTDRLGAPTYTWGSNHSDVFVLNNTRPPLGNWFGCTWMNSSGFTKVCGAPPCVIGGMGNNTLHCPTDCFRKHPEATYSRCGSGPWVTPRCLVHYPYRLWHYPCTINYTLFKVRMYVGGVEHRLEVACNWTRGERCDLDDRDRSELSPLLLSTTQWQVLPCSFTTLPALTTGLIHLHQNIVDVQYLYGVGSSIVSWAIKWEYVVLLFLLLADARICSCLWMMLLISQAEAALENLVLLNAASLAGTHGLASF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. We are looking for a protein with broad substrate specificity for a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-   diphosphate + H(+) + phosphate.2. A protein that can perform RNA binding, ribonucleoside triphosphate phosphatase activity, structural molecule activity, ATP binding, serine-type peptidase activity in ribonucleoprotein complex, viral envelope, host cell nucleus, viral nucleocapsid to support mitigation of host antiviral defense response, transformation of host cell by virus, clathrin-dependent endocytosis of virus by host cell.3. The designed protein must have at least one Peptidase S29, Peptidase C18 domain.4. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "VGSHYQLTNCCQEGDVLYSTKEFAYHLPGCVICTDDQCWDPVTVFLSVKPNGSTYDHHLRNHVLGLGVGAAVCDMLDINDQCGMLAVSLISLFDILPTHIQLNTTATCYLEVDYSPFGTIVGWVNWAVNEAGVVTWALSIVTRIPYAVFHLMELSSTPFLIAVVWFAIQGQWARLLLLAVLGRITNCCNRREISFCTRTLCWHKHGCVPCTTDGCWTPVGVTWSTLNATDRFDLWYHLDWVSGIVYTCDTLNIGEWCGAAAVIVELVVDTLQYHHDFVCNLTCFLFVDPMDSTAWFFEFVAMDSTWLKVLWSLVEGVPGLLLDFMTGGLTVSFLVMIAYTIEGKWTRALMLLFLG",
    "response#2": "VGSHYQLTNCCQEGDVLYSTKEFAYHLPGCVICTDDQCWDPVTVFLSVKPNGSTYDHHLRNHVLGLGVGAAVCDMLDINDQCGMLAVSLISLFDILPTHIQLNTTATCYLEVDYSPFGTIVGWVNWAVNEAGVVTWALSIVTRIPYAVFHLMELSSTPFLIAVVWFAIQGQWARLLLLAVLTTGGAQARAASGLAGFFSLGPSQKIQLVNSNGSWHINRTALNCNDSLNTGFLAALFYARKFNSSGCPERMASCRPIDRFDQGWGPITYVKPPSSDQKPYCWHYAPQPCGIVPASQVCGPVYCFTPSPVVVGTTDRSGVPTYRWGENETDVLLLNNTRPPRGNWFGCTWMNGTGFTKTCGGPPCNIGGGGNNTLTCPTDCFRKHPEATYTKCGSGPWLTPRCMVDYPYRLWHYPCTVNFTIFKIRMYVGGVEHRLTAACNWTRGERCDLEDRDRSELSPLLLSTTEWQVLPCSFTTLPALSTGLIHLHQNIVDVQYLYGIGSAVVSYAIKWEYVLLLFLYLADA",
    "response#3": "EVRNVSGVYHVTNDCSNASIVYEAADMIMHTPGCVPCVREGNSSRCWVALTPTLAARNSSIPTTTIRRHVDLLVGAAAFCSAMYVGDLCGSVFLVSQLFTFSPRRHETVQDCNCSIYPGHVTGHRMAWDMMMNWSPTTALVVSQLLRIPQAVLDVVAGAHWGVLAGLAYYSMVGNWAKVLIVMLLFAGVHHVTGSTVGQGVSQLTGLFTTGASQNLQLINTNGSWHINRTALNCNDSLQTGFLAGLFYRHKFNSSGCPERLARCKRLDEFAQGWGKLEAANITGSSQDRPYCWHYEPRQCGVVPATTVCGPVYCFTPSPVAVGTTDRAGLPTYNWGENETDVFILNSTRPPQGAWFGCTWMNGTGFTKTCGAPPCKLQVANASESWSCPTDCFRKHPETTYTKCGSGPWLTPRCLVHYPYRLWHYPCIVNFTIFKVRMYIGGVEHRLEAACNWTRGEPCGLEHRDRTEMSPLLLSTTQWQVLPCSFAPLPALSTGLIHLHQNIVDIQYIYGVGSAVVSWALKWEYVVLAFLLLADARVCACLWMMLLISQAEAALENLILLNAASVAGTHGILPF"
  },
  {
    "reference": "MIKKYLIGFVIFSFFSTLSYAQTKYEKAIFAGGCFWCMTPPFEKLKGVIKVESGYTGGTGENPTYHDYARKGHIEAVEVTYDPSKIKYSQLLDVFWRQIDPTDPAGQFCDRGPQYRSAIFYGTQEEKSLAQKSRDALEKSGRFSKPLVTEFIKASTFYRAEEYHQDYHRKNPVQYKFYRFNCGRDRYLNKVWRKDKMVKKFTGEELKKKLTPLQYKVTQENGTEQAFHNEYWDNHREGIYVDIVSGEPVFSSADKFESGTGWPSFTKPLEPENITEKQDNSFFMKRTEVRSKQGDSHLGHIFNDGPAPTGLRYCINSAALRFIPKEDLEKEGYGKYENLFEKQK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must have a active site, Nucleophile active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "EPLVTFIVGYEQMCLETNPGNNDVSLGDCSVKSASKVDQKWALYGDGTIRVNNDRSLCVTSEGKSSNEPIIILKCLGWANQRWVFNTDGTISNPDSKLVMHVDQNDVPLRKIILSHPSGTSNQQWIAN",
    "response#2": "NGDGNCLMHAASQYMWGVQDVDLVLRKTLYSALKEVDTRNFKLRWQRETIKSQEFVETGLHYDTRNWEEEWENIIKMTSTETPVARSGLQYNALEEIHIFVLVNVLRRPIIVLADKMLRSLESGSSFSPLNVSGIYLPLHWPAEECYRYPIVLGYDSMHG",
    "response#3": "NGDGNCLMHAASQYMWGVQDVDLVLRKTLYSALKEVDTRNFKLRWQRETIKSQEFVETGLHYDTRNWEEEWENIIKMTSTETPVARSGLQYNALEEIHIFVLVNVLRRPIIVLADKMLRSLESGSSFSPLNVSGIYLPLHWPAEECYRYPIVLGYDSMHG"
  },
  {
    "reference": "MAANEDRDDAAAILNWEGTSEIKSANEYSRNLLFSVLDSLDGNKTIVWDRDRSVMHRVNLFAGASVLAAHGVVANHSIETKKSASTPHVVFFLAPTMVSLDLLCDYIDNVRNDSKILYQVFFIPEAWFVVRESLKTRAEGKYWERLESVKEIPLCWLPRDGECLSLSSPQIAARLLINGDWTHLHKCAVALNQLIDMCRGRSSSSNQRPMSIYAKGKWASDVAKMMGKIRNSAEADSMTKNLDPIEGLLKINRIVLIDRWMDPLTPMLSQLTFYGLLDEIYGIGMVNSVKVPEMEFKNEKDGDPFQEKEVYLIDEVYHRLKHSHINAVSIEASKVLAEIRDDEQFDRDKMSVAEYSVLVKKMPKIINRKKMIEVHMRLAEMIQSHVYCKQSDSIKLERDLLEYSDSDKAIPLIEDLIFDASPLNAVLRLISVHSLTCGGLKPSVLQHYRRIVNQSYGSSALNKVLKMQKMGLIREKGGGGKMQCEYAQMMFQQMKKNHDMLPEEFSEAKLDDMAYAYSGFSPLLCKMLEEGDRVKWVGWPKTVIGDKSDLIAERDGRGTCVFVIGGLTRSELAIIRENLPNVALITTSALITGDKLLNNITNI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be designed to localize specifically to CORVET complex, lysosome, late endosome, apical plasma membrane, HOPS complex in order to perform its role in intracellular protein transport, vacuole fusion, non-autophagic, vesicle-mediated transport more effectively.2. For general function, the protein need meet that Required for early embryonic development.; Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport pathways; Believed to act as a component of the putative HOPS endosomal tethering complex which is proposed to be involved in the rab-5-to-rab-7 endosome conversion probably implicating sand-1, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE- mediated membrane fusion; Required for endosome/lysosome fusion; Within the HOPS complex, contributes to the normal development of gut granules in embryonic and adult intestinal cells; The HOPS complex is proposed to be recruited to rab-7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes  The designed protein sequence is ",
    "response#1": "PSSSVFLGDQQLYFTQEYLATNSLNMKYVVYFAACQFSGPIAVIYAAPKSWFIWIRTISGRILKRDLPCTDPVFIDWTRAHCLLVLSKNGRAQVLSSIGEKVSEVFFDNQVSDVHECRTFATSRGDSGIAVMDVDGQVAVVNSVSEPVIWSMRPPYSELPTAWTAFQPHSQLTHILLIFEAVFLMGCQGESLQVQNHASVWVDSSTKYVKCVVDDARSRIAMMTENGKIQIVSIDLSTCFCTVEVTEHEIGKCINFGWVGNSVVFVQMSSSLTVFVNVSARRKPGDEVRFMSIKMTANARISVEPDGIRLFESTRVEFVEAASREKIAVLNRSLNEDGAYLYKAAQEMEQGTGHNSFAASTVIQDMYKAIDDCISTACDTWQPEEQKLLLKAARFGMAYTNTTPDTTVNQTLLAGGRAGHLYAYTISANRRGFELTNICKSFHKKAVMELKVCQREDLLLCVSDGQLMAHKLSDPEYKVETLIHKVKPVQTFARFSPKTSGDLYVIVSSRKKLYLFKWGEKDGHKEFIEVALDYNPVFLDTPTSIRCVGEMVFFSVRNEYFSMTMQKDKTTTSPSEGSTPEGWNGFVTRLLNFNCQPGIVPMIDRRRVAFVRNEIVVTTDIWGQRPANVLSDEYKFSEVPMQIVYDSPYLVGMLSKGRVEVRSIFDGQLVQTMSLPKAMTLCSFK",
    "response#2": "PVEVTCLAFQESNQTLLAGGRAGHLYAYTISANRRGFELTNICKSFHKKAVMELKVCQREDLLLCVSDGQLMAHKLSDPEYKVETLIHKVKPVQTFARFSPKTSGDLYVIVSSRKKLYLFKWGEKDGHKEFIEVALDYNPVFLDTPTSIRCVGEMVFFSVRNEYFSMTMQKDKTTTSPSEGSTPEGWNGFVTRLLNFNCQPGIVPMIDRRRVAFVRNEIVVTTDIWGQRPANVLSDEYKFSEVPMQIVYDSPYLVGMLSKGRVEVRSIFDGQLVQTMSLPKAMTLCSGARGPVEVTCLAFQESNQTLLAGGRAGHLYAYTISANRRGFELTNICKSFHKKAVMELKVCQREDLLLCVSDGQLMAHKLSDPEYKVETLIHKVKPVQTFARFSPKTSGDLYVIVSSRKKLYLFKWGEKDGHKEFIEVALDYNPVFLDTPTSIRCVGEMVFFSVRNEYFSMTMQKDKTTTSPSEGSTPEGWNGFVTRLLNFNCQPGIVPMIDRRRVAFVRNEIVVTTDIWGQRPANVLSDEYKFSEVPMQIVYDSPYLVGMLSKGRVEVRSIFDGQLVQTMSLPKAMTLCSGARGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELETLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRLQRRLEDELGGQFLDLSLHDTVTTLILSGQNKRAEQLARDFRIPDKRLWWLKLTALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKYEAKKYASRVGPEQKVKALLLVGDVAQAADVAIEHRNEAEMSLVLSHCTGATDGATADKIQRARA",
    "response#3": "YTANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWRKEKPASARPVLEIYSASGVPLASLLWKSGPVVSLGWSAEEELLCVQEDGVVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLXSAPSCWTTVCQDRVAHILLAVGPDLYLLDHAACSAVTPPGLAPGVSSFLQMAVSFTYRHLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGDAPESIQFVLDEDSYLVPELDGVRVFSRSTHEFLHEVPVASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLPQAVQQCIEAAGHEHWPDMQKSLLRAASFGKCFLDRFPPDSFVTSTLPVRNLNQTLLAGGRAGHLYAYTISANRRGFELTNICKSFHKKAVMELKVCQREDLLLCVSDGQLMAHKLSDPEYKVETLIHKVKPVQTFARFSPKTSGDLYVIVSSRKKLYLFKWGEKDGHKEFIEVALDYNPVFLDTPTSIRCVGEMVFFSVRNEYFSMTMQKDKTTTSPSEGSTPEGWNGFVTRLLNFNCQPGIVPMIDRRRVAFVRNEIVVTTDIWGQRPANVLSDEYKFSEVPMQIVYDSPYLVGMLSKGRVEVRSIFDGQLVQTMSLPKAMTLCST"
  },
  {
    "reference": "MGGKWSKSSIVGWPDIRERIRRAGPPPAAEGVGAASQDLDKYGALTSSNTDTSNADCAWLRAQEEAEEVGFPVRPQVPLRPMTFKGAFDLSHFLREKGGLEGLIYSEKRKEILDLWVYHTQGFFPDWHNYTPGPGVRYPLTFGWCFKLVPVDPREVEEASEGEDNCLLHPVCQHGIEDEHREVLKWQFDIQLAHRHIARELHPEFYKNC",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should include a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif to ensure proper function.2. The protein design should aim to mimic or enhance the function of natural proteins involved in suppression by virus of host autophagy, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "GHYIP",
    "response#2": "EAVGAAAA",
    "response#3": "LNW"
  },
  {
    "reference": "MAVMLVTAEQMRLLDRRTIEDFGVPSFTLMQRAAAGAAEVLRRRFAPAQRRRILVVAGKGNNGGDGMVCARLLARRGTSVTVALLARPEDLKGDAARAFRELRETNVAILTNVDPRQLVSLLPQSTLLVDALLGTGLNAPAQGAMADAIVAMNSSGRPILSLDVPSGLDSDCGAPQGVAVCASATVTFGLTKVGQVIYPGRAFVGQLEVVDIGIPEVAVADVSPKSWLLEPVDIARRLPVRAPTAHKGTCGHVLVLGGSVGRTGAARMAAEAALRAGAGLVTLAGPASLHATLAAHCPEVMTFPLPDEDGNLRFDEGALRRALEGKNAVVLGPGMGTSDECLRVVRFILERPDLPVVCDADGLTLLARLPGAADYKRPNLVLTPHPGEFSRLTGKAVAEIQATRPRLAREFAAQRGCTLVLKGATTLVTEEGGTLWVNPTGNPGMASGGMGDVLAGLIGGFIAQGLRTVDASLVAVFVHGSAADALSRDYAAVGYLATDVARAVPRQLDALRGYQESLVKRPSA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a high affinity for (6S)-NADPHX, AMP, K(+).2. The designed protein must possess ADP-dependent NAD(P)H-hydrate dehydratase activity, NADHX epimerase activity, ATP binding, metal ion binding, NADPHX epimerase activity to support nicotinamide nucleotide metabolic process under certain conditions.3. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.. The designed protein sequence is ",
    "response#1": "ILEEHEVSALMKHRPDFANKGTMGHALLVVGSYGMSGAAILASRACMRSGVGKLTVHTPARNYDIMQISVPEAVLQLDKDDYEFSDSVVCDDFDALGIGPGLGQSENTAVALIAQISRAKCPVVADADAINILASHQAWIQQLPKGIILTPHPKEFDRLMGSASASCYDRLMRARQLASRLQGYIILKGHYSALCLPDGQVLFNPTGNSGMATAGSGDVLTGIITGLLARGYNQADACTIGMYLHGLAGDLAMKDLGKESLVASDIISYLPKAL",
    "response#2": "PDSHKGTFGHLLVVAGSEGMTGAAALAGEAAMRVGSGLVSVAVPQSLRTVIEMKLTECLKLGLEESEPGVLGEGAVRQLTPHLHRYHATLLGPGLSTRGEVARVVVALIERAEHPMVLDADALNILAGTLESLEVAKAPLILTPHPGEMARLLRTSTARVQENRLSIAHDFATRHGVVLVLKGARTLIATPEGEVFVNPTGNAGMASGGTGDVLAGMIGGFLVQGHPPRTAALLGVYLHGLAGDLAAEALGERSFIASDLLDRLGQALWAAVFPSLDLESHKYARGHAIVVSGPPARTGAARLGARGALRIGAGLVTVASEPDALAANAAQLTAIMVEPFQGAVGLSDLLADERKNAALIGPGAGVGDETRENVLALLATQAALVLDADALTSFETDSDTLFAAIKARAATVAITPHEGEFQRLFSGAISEGSKLDRSRRAAEMSGAVVILKGADTVVAAPDGRAAINENAPPWLATAGSGDVLAGFITGLLAQHMPAFEAAAAAVWLHGAAAEAFGPGLIAEDLPESLPECLAGLTAA",
    "response#3": "MQELDRQTIQEHGIPGLELMEHAGHACMEAIFHEFGYTGGKRAVIFAGRGNNGGDGYVIARLLLQSGWQVRVCILAERERIGGDAAINLEQLPPAAVSYCTVPGQLAGLLQEELSQTDVIVDALFGTGLNSEVAGINREAVELINAAGCPILAVDIPSGIHGTSGRVLGCAVRATVTVTFAAAKLGHVLYPGADHTGRLVVADIGIPAELVR"
  },
  {
    "reference": "MAPSPSKRKERSEDKAKERGKEKAPGKEATDKDRGRDKAKKRRSASSGSSSSSRSRSSSSSSSSSGSSSGSSSGSSSSASSRSGSSSSSRSSSSSSSSGSPSPSRRRHDNRRRSRSKSKQPKRDEKERKRRSPSPRPTKVHIGRLTRNVTKDHILEIFSTYGKIKMIDMPVDRYHPHLSKGYAYVEFEAPEEAEKALKHMDGGQIDGQEITASAVLTPWPMRAMPRRFSPPRRMLPPPPMWRRSPPRMRRRSRSPRRRSPVRRRSRSPARRRHRSRSSSNSSR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.2. The protein contains novel RRM domains that confer a unique function or activity.3. The protein must exhibit the following characteristics: Also mediates increase of mRNA abundance and translational efficiency ..4. The protein should be designed to localize specifically to cytoplasm in order to perform its role in RNA splicing more effectively. The designed protein sequence is ",
    "response#1": "SPYGDPIDPEDAPESITRAVASLPPEQMFELMKQMKLCVQNSHQEARNMLLQNPQLAYALLQAQVVMRIMDPEIALKIL",
    "response#2": "SPYGDPIDPEDAPESITRAVASLPPEQMFELMKQMKLCVQNSHQEARNMLLQNPQLAYALLQAQVVMRIMDPEIALKIF",
    "response#3": "SPYGDPIDPEDAPESITRAVASLPPEQMFELMKQMKLCVQNSHQEARNMLLQNPQLAYALLQAQVVMRIMDPEIALKIL"
  },
  {
    "reference": "MTTKHIFVTGGVVSSLGKGLTSASIGLLLEQRGLKVRMQKLDPYINVDPGTMSPFQHGEVYVLDDGSETDLDLGHYERFTNSPLSRKSNYTTGQIYSSVIEKERRGDYLGNTVQVVPHITDAIKEAVINLGGPDVDVVITELGGTVGDIEGLPFLEAIRQIPLDVGKNNCLFIHLTLVPYLKAAQEAKTKPTQHSVGQLRQIGIQPDILIVRTERELGRDNCEKIALFCNVEKQAVIEERDKDFSIYEVPLGLVESKLDQLIVDKLALPAQPIDISEWRNLIHRLRNPQHEVTIAVVGKYIEHRDAYKSIYESLEHAGIANSTRVLVKRVDAEALEAEGPEILLQGVDGILCPGGFGERGIEGKVEAIRFARECEIPYFGICLGMQCAVIEFARNVLNLTNAHSSEFADEAEHPVICLLDEQRSVTTKGATMRLGTQPCRLAEDSRSAACYETTEVSERHRHRYEFNPDYRQQFIEAGMTPSGTSPDGKLVEIVEVPAHPWFVAVQFHPEFKSKPTAAHPLFSGFVEAAIKYHHQRQHQAQF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen2. The protein's Nucleophile, Nucleophile; for glutamine hydrolysis must be carefully engineered to ensure that the amino acids involved in H2O + L-glutamine = L-glutamate + NH4(+) are properly positioned for optimal catalysis.3. The designed protein should have glutaminase activity to facilitate 'de novo' CTP biosynthetic process.4. The L-glutamine binding site should be located in a region of the protein that is accessible to the ligand.5. The presence of Glutamine amidotransferase, CTP synthase N-terminal domains is critical for the interaction of the designed protein with its intended targets.6. We require a protein that can specifically bind to the metabolites produced in the Pyrimidine metabolism, CTP biosynthesis via de novo pathway pathway. The designed protein sequence is ",
    "response#1": "MGKGLTAASLGNLLTARGLRVVMQKLDPYLNVDPGTMNPFQHGEVFVTDDGAETDLDIGHYERFLDINLSQAANVTTGQIYSQVIAKERRGEYLGDTVQVIPHITDEIKRRMRLQAQDDPQPDVIITEIGGTVGDIESQPFIESARQVRHELGRRNVFFVHVSLVPFMGASGEQKTKPTQHSVAALRSIGIQPDALVLRSDRPVTSANRRKIALMCDVEEAGVVNAVDVPSIYDIPSMLHSQGLDAYIIDQLDLPLGQAPDLSQWENIVDRIKSPEKEVRIAIVGKYIEIKDAYLSIKEALKHAGIDSGCLVDIKWVEAEELEKMDIDEVREVFSDVEGILVPGGFGTRGSEGKIKAIRYARENKIPFLGLCFGFQLAVVEFARHVLGLEEAASAEVSETPYPVIEILPEQRNVRNMGGTMRLGDAEITLEEGTRAYEIYGKSKIIERHRHRYEVNPRYIEAIEKKGMVFSGKCGNRMEIAEIPDHPFFIGTQFHPEFKSRPDNPSPVFRAFIEAALERS",
    "response#2": "MGKGLTAASLGNLLTARGLRVVMQKLDPYLNVDPGTMNPFQHGEVFVTDDGAETDLDIGHYERFLDINLSQAANVTTGQIYSQVIAKERRGEYLGDTVQVIPHITDEIKRRMRLQAQDDPQPDVIITEIGGTVGDIESQPFIESARQVRHELGRRNVFFVHVSLVPFMGASGEQKTKPTQHSVAALRSIGIQPDALVLRSDRPVTSANRRKIALMCDVEEAGVVNAVDVPSIYDIPSMLHSQGLDAYIIDQLDLRSEPPDLRDWDALVARIHSPTREVRIAIVGKYIEIKDAYLSIKEALKHAGIDSGCLVDIKWVEAEELEKMDIDEVREVFSDVEGILVPGGFGTRGSEGKIKAIRYARENKIPFLGLCFGFQLAVVEFARHVLGLEEAASAEVSETPYPVIEILPEQRNVRNMGGTMRLGDAEITLEEGTRAYEIYGKSKIIERHRHRYEVNPRYIEAIEKKGMVFSGKCGNRMEIAEIPDHPFFIGTQFHPEFKSRPDNPSPVFRAFIGAVMDAVG",
    "response#3": "MGKGLTAASLGNLLTARGLRVVMQKLDPYLNVDPGTMNPFQHGEVFVTDDGAETDLDIGHYERFLDINLSQAANVTTGQIYSQVIAKERRGEYLGDTVQVIPHITDEIKRRMRLQAQDDPQPDVIITEIGGTVGDIESQPFIESARQVRHELGRRNVFFVHVSLVPFMGASGEQKTKPTQHSVAALRSIGIQPDALVLRSDRPVTSANRRKIALMCDVEEAGVVNAVDVPSIYDIPSMLHSQGLDAYIIDQLDIQASYPDLSKWEQVTIGLIGKYVELQDSYKSILEAFIHAGAENEVKVKVESIHSEHLTIKNSAKKLGHLDAVLVAPGFGERGVEGKIEAIKYVREQNIPFLGICLGMQMAVIEFSRNVLGLKNANSTEMNETTEYPVISLMESQKAVTDKGGTMRLGAWDCNILDNSIAKNVYKTNAIKERHRHRYEFNNDYKSQIEKAGMKATGLNPETGLVEIVEIPTNNWFVGVQYHPEYKSTVANPHPLFVAFVKAALNYSN"
  },
  {
    "reference": "MKIGQIRQSLSGFYDVYADGQMYRTRARGNFRKRRITPLVGDNVEFDAATPQEGYVLNILDRQTQLVRPPVANVDLGIVVTATTEQEFSTNLLDRQLVALAVAGIEPLLYFAKTDLLTDEVYQQRLTLADAYRQIGYQVICERTAFSPAALAAVKTALAGHVAVVMGQTGAGKSTLLNHLQPGLALATGEISQALNRGKHTTRKVSLIPIADGLVADTPGFSSYEVFDIAANELTHYFPEFVRLSVDCKYRGCVHINEPQCAVKQALAAGELLASRYDNYLQFYETIKNKKVIYNKKK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit; Helps release RbfA from mature subunits; May play a role in the assembly of ribosomal proteins into the subunit.2. The protein can be expressed in cytoplasm and perform rRNA binding, metal ion binding without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "RGQLKPVAANIAQILIVFAVEPAPHPNLIDRYLVAAEATGITPVLVLNKTDLLPETGSDLRTLLARYRAIGYRVVAASTQQEGGLDALHACLTERTSVFVGQSGVGKSSLIDRLLPDMQLRVGALSADSRKGTHTTTTATLYHLPAGGELIDSPGIREANIAQILIVFAVEPAPHPNLIDRYLVAAEATGITPVLVLNKTDLLPETGSDLRTLLARYRAIGYRVVAASTQQEGGLDALHACLTERTSVFVGQSGVGKSSLIDRLLPDMQLRVGALSADSRKGTHTTTTATLYHLPAGGELIDSPGIL",
    "response#2": "QPRQSVLERPDARGQLKPVAANIAQILIVFAVEPAPHPNLIDRYLVAAEATGITPVLVLNKTDLLPETGSDLRTLLARYRAIGYRVVAASTQQEGGLDALHACLTERTSVFVGQSGVGKSSLIDRLLPDMQLRVGALSADSRKGTHTTTTATLYHLPAGGELIDSPGIREFGE",
    "response#3": "QPRQSVLERPDARGQLKPVAANIAQILIVFAVEPAPHPNLIDRYLVAAEATGITPVLVLNKTDLLPETGSDLRTLLARYRAIGYRVVAASTQQEGGLDALHACLTERTSVFVGQSGVGKSSLIDRLLPDMQLRVGALSADSRKGTHTTTTATLYHLPAGGELIDSPGIREFGL"
  },
  {
    "reference": "MTKYIFVTGGVTSSLGKGIISASLAKLLQARGYRITIQKFDPYINIDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLDAPTSQANNITTGRIYQNVINKEREGAFLGKTVQVVPHITDEIKRNIRILGENGQYDIVITELGGTVGDIESLPYIEAVRQFRWEIGTGNSLVIHLTLIPFLAAAGELKTKPTQHSVKTLLEYGIQPDILVCRTEHHLSQEIRKKIALFCNVNTNAVIESIDASTIYDVPLLMLKEQLDKIVLSKLKLTKNQEPDLESWKDFLGRLKNPTAEVQIGLIGKYVELPDAYKSINESFIHAGAKNECKVRVKCIHSEDILPENVADKLAGLHGILVAPGFGSRGIEGKITAIQYVRENNIPFFGICLGMQCAVIEFGRNILGWKDAHTTEIDERTLHPVISMMEEQKKIRNKGGTMRLGSYKCDIKKGTKAMAIYGKSTITERHRHRFEFNNKYLNDYENAGLIASGMNPDNNLVEIIELKNHPFFVAGQFHPELKSTVTNPHPLFVNFVAASLAHARKK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. We require a protein that can specifically bind to the metabolites produced in the CTP biosynthesis via de novo pathway, Pyrimidine metabolism pathway.2. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.3. The designed protein should have a binding site that is specific to the target CTP, Mg(2+), ATP, UTP ligand.4. The designed protein should have CTP synthase activity, ATP binding to facilitate 'de novo' CTP biosynthetic process.5. The active site of the protein must be engineered to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+). The designed protein sequence is ",
    "response#1": "MTGGVVSSLGKGITCASLGMLLKSRGLKTTIMKIDPYINVDPGTMSPYQHGEVYVTEDGAETDLDLGHYERFIDENMSQMNNITTGQIYNTVISKERRGDYLGATVQVIPHITDEIKSRIVLLARQSNADVIIVEVGGTVGDIESQPFLEAIRQFCLEYGSKNTVKIHLTLLPYISSAGEIKTKPTQHSVMRLREIGIQPDFLICRTDRTLDESAKAKIALFCNVEAQGVIEAIDVETIYEVPLRLAAQNLDDLVIEKLGLGESAPDLSRWKHVEIAICGKYTQLHDAYKSIMEAFVHAGVANDAGIRLRWVDSEEIERQGVEPFLTGVSGILVPYGFGHRGIEGKLAAIRYARENKVPFFGICLGMQCAVIEFARNVCGLKHANSSEFDPGTPDPVIHIMESQKAVQQKGGTMRLGTYPCVTVKKTLAQKIYQSEIIIERHRHRYELNNDYRRIFQEHGMVLSGLSPDGNLVEMVELGDHPFFVGCQFHPELKSRVMRPHPIFVHFIKAAIEKK",
    "response#2": "MTGGVVSSLGKGITCASLGMLLKSRGLKTTIMKIDPYINVDPGTMSPYQHGEVYVTEDGAETDLDLGHYERFIDENMSQMNNITTGQIYNTVISKERRGDYLGATVQVIPHITDEIKSRIVLLARQSNADVIIVEVGGTVGDIESQPFLEAIRQFCLEYGSKNTVKIHLTLLPYISSAGEIKTKPTQHSVMRLREIGIQPDFLICRTDRTLDESAKAKIALFCNVEAQGVIEAIDVETIYEVPLRLAAQNLDDLVIEKLGLNAKPLDLNEWRELVERIKSAKDSVRIAMIGKYTKLHDSYLSIIEALKHAGAWNNTGIELGWFEATDIENGKLSLEKLLEYDGAVILPGFGKRGAEGKIKAIRFLRENNKPLLGICFGMQLMVVEYARNVVGLERAHSTEIDPNTPYPVIDLLPSQYNVKHLGGTMRLGAHPIHIKKNTIAWQIYRREIVYERHRHRYEVNPKYIDKLENAGLKISGWSPEKYPEFIELPKYRTLYFGSQPHPEFKSRPLTPAPIYYYFLRAAASKKG",
    "response#3": "MTGGVVSSLGKGITCASLGMLLKSRGLKTTIMKIDPYINVDPGTMSPYQHGEVYVTEDGAETDLDLGHYERFIDENMSQMNNITTGQIYNTVISKERRGDYLGATVQVIPHITDEIKSRIVLLARQSNADVIIVEVGGTVGDIESQPFLEAIRQFCLEYGSKNTVKIHLTLLPYISSAGEIKTKPTQHSVMRLREIGIQPDFLICRTDRTLDESAKAKIALFCNVEAQGVIEAIDVETIYEVPLRLAAQNLDDLVIEKLGLDVPEPDLAEWKSMLDKLKNPKSELPDAYLSVTEALRAGGFHHDARVAIRWVASDTCQTPEGAQEALGGVDAVLVPGGFGVRGIEGKLGALRWAREHKVPTLGICLGLQCMVIEAARNLAGIEGASSSEFDPETPAPVIATMAEQKAFVEGAGNLGGTMRLGAYPAKLAKGSVVAGAYGATKASERHRHRYEVNNAYRARLEEAGLVFSGTSPDGTLVEFVELPRDVHPYYVSTQAHPEFKSRPHRAHPLFAGLVAAALERKRSR"
  },
  {
    "reference": "MVARLTTLLVCLVFSLATLVQRGYGDFDDFNLEDALKETSSVKQSHFSTTTRRTGTTRAPANPAERWDHMTTTTTKRPGTTRAPSNPLELDGFDLEDALDDRNDLDGPKKPSTGEGGGLSDKDLEDILGGGGYKPDKNKGGGGGYGSQDDPGSGAVTDPGTIAGLVSALAAALLGAVSGYLSYQHRKFCFSVQRGLDAAYVKGENLEAVVCEEPRVEAAMCEAPPVTDSTQHSQPTEPLAPERPRI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The designed protein should have a specific function within the plasma membrane, cell surface.3. The designed protein should contain Cytoplasmic topological domains.4. The protein must exhibit the following characteristics: Acts at the same site as, but independently of, PECAM1 . The designed protein sequence is ",
    "response#1": "MQAMRFAVEEINNDSSLLPDVRLGYEMVDVCYVSNNVQPVLYFLAQEDNLLPIQEDYSNYVPRVVAVIGPENSESVTTVANFLSLFLLPQITYSAISDQLRDKQRFPALLRTTPSAKHHIEAMVQLMLHFRWNWISVLVSSDTYGRDNGQLLGDRLAGGDICIAFQETLPTLQPNQDMMPEDRQRLVSIVEKLQQSTARVVVVFSPDLTLYDFFREVLRQNFTGAVWIASESWAIDPVLHNLTGLHRTGTFLGITLQNVPIPGFNEFRVRGPQAGPTHQRSTCNQECDTCLNSTLSFNTILRLSGERVVYSVYSAVYAVAHALHSLLGCDHSACTKRGVYPWQLLEEIWKVNFSLLDHQIFFDPQGDVALHLEIVQWQWRXM",
    "response#2": "GPEQAAVLNCSLGAAAAGPPTRVTWSKDGDTLLEHDHLHLLPNGSLWLSQPLAPNGSDESVPEAVGVIEGNYSCLAHGPLN",
    "response#3": "GPEQAAVLNCSLGAAAAGPPTRVTWSKDGDTLLEHDHLHLLPNGSLWLSQPLAPNGSDESVPEAVGVIEGNYSCLAHGPLV"
  },
  {
    "reference": "MVKAKKFVLVNGFNNMPKEEDFKLVEEDLLGPQEGEFLAEALYLSVDPYMRAYAHQLKEGKTMIGVQVARILESNNKEFPVGKYVVGNFGWSTHTISNGLRSTTQSEVDHYPYVLPDIGQLPPSLGLGVLGLTGNTAYFGFTEICKPRAGETVVVTTAAGAVGSHVGQIAKIKGCRVIGITGSDEKCLWLKSIGFDAAINYKNANWINALSEATPRGIDCYFDNVGGEISSTIIQRMCTDGRVAVCGSISSYNCDVRQLPKATMIQPWIVFHQLEIKGFVSNNWSRRWMEGIKENLKWVQEGRLRYKETITDGFENMPKAFIGLLKGENIGKAIVKV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be designed to catalyze the following reaction leukotriene B4 + NADP(+) = 12-oxo-leukotriene B4 + H(+) +   NADPH. The designed protein sequence is ",
    "response#1": "GDEPCSLGLDKIKPYVLPDIQGLSPSLGLGFLGMPGNTAYFGFLEICKPKAGETVVVNAAAGAVGMHVGQIAKIKGCRVIGFAGSDDKVEWLKKEIGFDAAYNYKTVDIDKALAESAPNGVDCYFDNVGGEMSSIVMNRMNHFGRVSVCGAISSYNDAVKPKATLIQGMVNRSELKIEGFMVYRWEERWLEGIKQNIQWIKEGKLKYQETITNGFEKIPQAFIEMLEGKNFGKAIL",
    "response#2": "GFPKDSDFELKTEQLSEPKDGEVLLEAVFLSVDPYMRPYSRVRMQAGDVMIGTQVAKLTALYGLEEVCAIKPGETLLVNAAAGAVGSVAGQIAKLKGCKVVGSAGSDEKVAYLKEMGFDQAFNYKTISSLEEALKNASPEGYDCYFENVGGPFSSVAIAQMKAFGRIAVCGGISLYNDTSPQTGPYPHLHMIFKQLKMEGFLVSRWEHKNKESLKRMLTWMQEGKLKCKEHVTVGFENMPAAFMGMLRGENIGKAIL",
    "response#3": "GFPKDSDFELKTEQLSEPKDGEVLLEAVFLSVDPYMRPYSRVRMQAGDVMIGTQVAKLTALYGLEEVCAIKPGETLLVNAAAGAVGSVAGQIAKLKGCKVVGSAGSDEKVAYLKEMGFDQAFNYKTISSLEEALKNASPEGYDCYFENVGGPFSSVAIAQMKAFGRIAVCGGISLYNDTSPQTGPYPHLHMIFKQLKMEGFLVSRWEHKNKESLKRMLTWMQEGKLKCKEHVTVGFENMPAAFMGMLRGENIGKAIL"
  },
  {
    "reference": "MLASGLLLAALLACLTVMILLSVWRQRKLWGKLPPGPTPLPFIGNYLQLNTEQMYDSLMKIRDRYGPVFTIHLGPRRIVVLCGQEAVKEALVDQAEDFSGRGEQATFDWLFKGYGVAFSTWERARPLRRFAISTLRDFGVGKRGIEERIQEEAGFLIEAFRDTRGAFIDPTFFLSRTVSNVISSIVFGDRFDYEDKEFLSLLRMMLGSFQFTATPTGQLYEMFYSVMKHLPGPQQQAFKELEGLRDFIAKKVERNQRTLDPNSPRDFIDSFLIRMQEEKKDPKSEFHMKNLVLTTLNLFFAGTETVSTTMRYGFLLLMKHPDVEAKVHEEIDRVIGRNRQPKFEDRAKMPYTEAVIHEIQRFTDMIPMGLARRVTRDTKFRDFLLPKGTEVFPMLGSVLKDPKFFSKPREFYPQHFLDEKGQFKKSDAFMPFSVGKRYCLGEGLARMELFLFFTTIMQNFRFRSQQAPQDIDVSPKHVGFATIPRTYTMSFVPR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must have high specificity towards aromatase activity.2. The designed protein should be able to compete with endogenous proteins or inhibitors for heme binding. The designed protein sequence is ",
    "response#1": "LSDEEWKKVVDIWGKVEPDLPSHGQEVIIRMFQNHPETQDRFAKFKNLKTLDEMKNSEDLKKHGTTVLTALGRILKQKGHHEAEIAPLAQTHANTHKIPIKYLEFICEVIVGVIAEKHSADFGADSQEAMRKALELFRNDMASRYR",
    "response#2": "DVIRPFSQPMYPQGHLGILKGNLALEGAVSKLTGIKNRSITGPARVFESEEDCLDAILANKIKAGDVIVVRYEGPKGGPGMREMLAPTSAIIGAGLGDSVGLITDGRFSGGTYGMVVGHVAPEAAVGGTIGLVKEGDSITIDADKLLLQVNVSDDELAERKTHWSPPKPRYERGVLAKYAKLVSSSSKGG",
    "response#3": "DVIRPFSQPMYPQGHLGILKGNLALEGAVSKLTGIKNRSITGPARVFESEEDCLDAILANKIKAGDVIVVRYEGPKGGPGMREMLAPTSAIIGAGLGDSVGLITDGRFSGGTYGMVVGHVAPEAAVGGTIGLVKEGDSITIDADKLLLQVNVSDDELAERKTHWSPPKPRYERGVLAKYAKLVSSSSKGG"
  },
  {
    "reference": "MRVALKLAYIGTEFHGSQIQPSVETVEKELFKALRNLRIIESPKSANYICAGRTDAGVHALGQVIAFDTDRPNLAIPRIINSGLPPTIWVWAHAEVPYDFDARRHAVSRHYRYVISGEGYDISKMREASKLLLGTHDFENFSRSNGEKSTVRTLERINVRVDGDITKIDVVGNSFLWNMVRKIVTALSMIGKGVRDNDWLIQMLNPDIYEEGIEPAPAYGLTLMGVNYGEEINWIEDNYSIRRASDQNSKRILRYRVMAEVLEELIYHE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The desired protein should be able to perform uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA2. For general function, the protein need meet that Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.3. The protein should be able to bind substrate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "AASLTIGLHDFGSFATPNPGGTTIREVKTAYWRRVPITPLVPDEMASHEAYRTPSLESGLVVFTIVADAFARNMVRSLVGSCIKVGSGRKSLEWFAGKMAEPVREGSSGPIAPQGLTLEHIAYK",
    "response#2": "AASLTIGLHDFGSFATPNPGGTTIREVKTAYWRRVPITPLVPDEMASHEAYRTPSLESGLVVFTIVADAFARNMVRSLVGSCIKVGSGRKSLEWFAGKMAEPVREGSSGPIAPQGLTLEHIAYR",
    "response#3": "AASLTIGLHDFGSFATPNPGGTTIREVKTAYWRRVPITPLVPDEMASHEAYRTPSLESGLVVFTIVADAFARNMVRSLVGSCIKVGSGRKSLEWFAGKMAEPVREGSSGPIAPQGLTLEHIAYR"
  },
  {
    "reference": "MMAALGVGSGQLLRRHALANIRGRCSRCADQHVTTLMLSSKYPLRTPIATRNFSSRSGRRPRFSQRLGEALRNSKITWYHIPVGVGIGFLGLVQFYKVTAREQEKRRREEGGVPTEQPKRRHRIRPDGPWQVQVMSTLPLKAISRLWGKFNELTIPYYLRVPGFKLYSFIFGVNPRKFLISVQSLEEVSEPDLHAYPNLAAFFYRTLKPGARPLDPDSRALLSPADGKILQYGRIEGGDIEQVKGMTYSVDALLGKNSPTASIASGQSSASAQSGGSVAHGDEEIVKQEEEFAKMNGISYTLPDLLSGQPDAQKPHSATDESTEVSAGAVSEVRAELALGEKPWYDIVSADKTTSLYYAVIYLAPGDYHRFHSPTNWVVERRRHFAGELYSVSPYLQRTLPGLFTLNERVVLLGRWRYGFFSYVPVGATNVGSIKINFDRELRTNSLLTDTAADHAAEEAAKRGEPYMGYAEATYEAASPILHGHALRRGEEMGGFQLGSTIVLVFEAPSEKAQGGKPSGGWHWAVEKGQKIKMGQALGRVVDE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.3. The designed protein should have phosphatidylserine decarboxylase activity to facilitate protein autoprocessing.4. For general function, the protein need meet that Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer); Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.5. The protein design should incorporate a binding pocket that specifically recognizes pyruvate to enhance enzymatic efficiency.6. We require a protein that can specifically bind to the metabolites produced in the phosphatidylethanolamine biosynthesis pathway. The designed protein sequence is ",
    "response#1": "LKVSVLRGRNLAAKDRGGTSDPYLIVTLGDARQSTPTIFKTLNPEWNVTFEMPVVGVPLLECICWDHDRFGKDYLGEFDIPLEDIFSDGEINQEPKWYDEDDDEVMHDRARSPSPLPSNLREISSLASWTVSTHKPGCGVAALRHPSPTQYWQSDGPQPHTLTLHFFKLVAVVKIRVYLDFDLDESYTPTKMTFLAGMGGNDLVEFATWEDETPCGWVDVPLEGVGGRDGGWVRTKRRNRARKGGMGKSSAWPDYMFSDTENETNLKAKNLAVRISYAKHVDLIDNFQCNPICFVTTNTFYTKRTAKLKNSNTHWNQILKLKLPRTPQSDLLRVIVFDALPTMAPTTTASTPPLSNTSSVTSMASMTSSFGGGSQQLYGRSSTTIPNSMMVSNASIPDYHDSSDPVHHHNTGVDHLNIQHTYSNQSLDHSNPDHYKRHVTSNYLYIGEVQLSLLDLFKKKGSKNLDEDDDEVMHDRARSPSPLPSNLREISSLASWTVSTHKPGCGVAALRHPSPTQYWQSDGPQPHTLTLHFFKLVAVVKIRVYLDFDLDESYTPTKMTFLAGMGGNDLVEFATWEDETPCGWVDVPLEGVGGRDGGWVRTKRRNRARKGGMGKSSAWPDYMFSDTENKTKLTAQLNKQLKQPFSITRNQVTDLLGFTFVEIDSISNLPPLKNVFHTGFDMDPFVIMSFSKNTFRTKRVRHNLNPVYNQKFLFEVGTLEKNYDIIFKVIDHDKISLNDSVGACSVDIQSIINASAQPDP",
    "response#2": "VMFTEFVSITDLPPLKSKLSRTFDMDPFIVASFGRKVFKTSWRKHTLNPTFGESAAFEVFPNEKNFSFHFRVLDKDSFTFNDEVAEYDLPWTDMIEKLQNKDGTLVVRVHAGRDLVAKDKNGLSDPYLVIRFGPHRITSPVVPKTLNPVWGAGGETGSLEGVGGEAKLELKLFDCLALGYERVEIVCYDKDRLGSEYMGELSLGLEDGADADDSGRIDKVEILVQIEITGAKDLPRFKNAFRTSYDMDPFVVIAFGHKIFRTRVIRHSLNPVWEERLYFHVGEAETHWTIAFTVSDWDKISGNDHVGDVAVPLEQLLGTSIQHDGEK",
    "response#3": "TPGQSRSTSPMRSGDPKPLVLKVSVLRGRNLAAKDRGGTSDPYLIVTLGDARQSTPTIFKTLNPEWNVTFEMPVVGVPLLECICWDHDRFGKDYLGEFDIPLEDIFSDGEINQEPKWYPVARAGTGGLKVYVIKARNLAPKDRSGTSDPYLVVTLGNAKQSTPSISKNLNPEWKICFDMPLTGVPLLECMCWDKDRFGKDYMGEFDIPIEDIFTNGQIQTQPKWYKPVEKEGAVDADDSGRIDKVEELKVVVLRGRCLAAKDRNGTSDPYLVVTLGDARQSTPTIPKTLNPEWNVTFEMPIVGVPLLECICWDHDRFGKDYMGEFDIPLEEIFAEGQIHQQPKWYTDEDDDEVMHDRARSPSPLPSNLREISSLASWTVSTHKPGCGVAALRHPSPTQYWQSDGPQPHTLTLHFFKLVAVVKIRVYLDFDLDESYTPTKMTFLAGMGGNDLVEFATWEDETPCGWVDVPLEGVGGRDGGWVRTKRRNRARKGGMGKSSAWPDYMFSDTENTLVVRVHAGRDLVAKDKNGLSDPYLVIRFGPHRITSPVVPKTLNPVWGAGGETGSLEGVGGEAKLELKLFDCLALGYERVEIVCYDKDRLGSEYMGELSLGLEDWWGSDAKKWYPVAQAGTGGTGQGRGSVFMEIVRVTDLPPERNVTRTSFDMDPFVVTSLGRKTLRTPVVRHNLNPVYHEKMVFQVMKHEQSYTISFTVMDRDKFSGNDFVASASFPLQTLVQA"
  },
  {
    "reference": "MTESRNVVIGVSGGIAAYKACHLIRDFKERGDDVRVVPTPNALNFVGAATFEALSGNPVDTGVFTRVDEVQHVRVGQEADLIVVAPATADVVARIAAGRADDLLTSTILVATCPVVVAPAMHTEMWLNPATQANIRTLRVRGITVLEPAHGRLTGKDTGPGRLLEPVQIAELARTVAETGPLGRGLEGKQVLISAGGTQENIDPVRFIGNRSSGRQGFALAEIAAHKGARVTLVAGDTDELYAPAGADVVRVRSTRDLEVEMNARAVDADVIIMAAAVADFRPASESASKMKKGRDTDALTRIELVENPDVLRGLVDQRRAGETAATIVGFAAETDNVLDNGKAKLKRKGADMLMVNSVAGGAVFGQARNSGWLLGSDGSVREVPDGPKHVVAARIWDAIEAFGLPDPRELAN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domain.2. The protein should be able to bind CTP ligand in a variety of conditions3. A protein with phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity, FMN binding functions is required to enable pantothenate catabolic process.4. We need a protein that can perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2 with high efficiency.5. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A.6. A protein that can modulate the overall flux of metabolites through the CoA from (R)- pantothenate: step 3/5 pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "KVVLGVTGGIAAYKTAELARIFIRSGVEVQVVMTEAASRFVNPLTFTTLTGNPVHFQMYDGQSTGIRHIDLSEGADAMIIAPATANTIAKMATGLADNLLTTLFLATLCPVIIVPSMNVKMFEHPAVQNNLQVLRSRGCHILEPNAGELACGEYGRGRMPEPQEIFDLGLSALQELRLAGRRVIVTAGGTREPIDPVRFIGNRSSGKMGIAMADAAYARGAEVTLVSTVEVDRAYSVLLVETALEMQETVEAEFDGCDALVMTAAVADFRPLGFSNQKIKKSVKEDFSLELTKNPDILDLLGRVKTDEQFIIGFAAESEELLSNASQKLKRKNLDVIVGNDITVPDIGFGSDDNAVIISVDGTTVALREGSKHDLAAAVWDAVTAVR",
    "response#2": "MAGLKVVVSAGPTREYLDPVRFISNPSSGKMGFAVAQVAASRGAEVVLVSGPVNMSTPTGVRRRDVESALDMQRAVEEAAAAADLVVMTAAIADYRAADLKTEKEAKGGETKTIELVKNPDILAGLGEKKGERVLVGFAMETHAGVERAADKARRKNADFILLNYPTQEGTAFGSDDNQVTLVLEDASHQVLREPRTSKEAVATALVAVARTRLDA",
    "response#3": "KVVLGVTGGIAAYKTAELARIFIRSGVEVQVVMTEAASRFVNPLTFTTLTGNPVHFQMYDGQSTGIRHIDLSEGADAMIIAPATANTIAKMATGLADNLLTTLFLATLCPVIIVPSMNVKMFEHPAVQNNLQVLRSRGCHILEPNAGELACGEYGRGRMPEPQEIFDVALALLRHRDLAGRRVLVTAGPTREAIDPVRFVSNRSSGRMGYALAEVAWRRGAAVTLVSGPTALPPPVGVELVRVETAAAMAEAVAAHLPAADVLVMAAAPADFRPAAPAAAKLTKEDGLPPPLAWALTADILQSTRPLRRPDTRVVGFALETADDALARARAKLEAKGLDLVVLNRADEADAGFDTETNRVTVLTADGETELPRMGKYALAHRILDAVADALPPREPRP"
  },
  {
    "reference": "MSKYIFVTGGVASSVGKGISVASLGRLLKNRGISVSIMKLDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTDEPSYQANNVTTGQVYASVIGKERRGEYLGGTIQVIPHITNEIKERMHAVARRSDADVVIXXXAIRQMRKDVGRTNVLYLHVTLLPYIGATKELKTKPTQHSVKELLRVGIQPDVILCRSDYPMTDELREKIALFCNIEARAVVPMLTAETIYEVPLILEEAGLGEYMVQELGLPHREPHMQEWRELVEKIKRPRPELTIGIVGKYVELHDAYLSVAESLRHAGWYNDVDINIKWINSEALDEMGEGAVEVLEALDGIVVPGGFGHRGIEGKIKAANFARRMKKPYLGLCLGMQVAVVEFARNVLGLEGANSTEFDPQTPYPVIDLMNTQRTVADKGGTMRLGNWVCCLTPGTKAYQAYGEPIVFERHRHRYEFNNEYRKRMETHGVIVSGRSADNSLVEIIELADHPWFVASQFHPEFKSRPNRPHPLFRDFIGTAVRLAKEPDAKIEQEVGVGTGTGTRSVFGSAGLADHSNSHSAAQADVRIEK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have a binding site that is specific to the target UTP, L-glutamine, Mg(2+), CTP, ATP ligand.2. The designed protein is the optimization of the Nucleophile, active site, Nucleophile; for glutamine hydrolysis to achieve high catalytic efficiency for H2O + L-glutamine = L-glutamate + NH4(+).3. The protein design should consider the endogenous proteins and enzymes present in the CTP biosynthesis via de novo pathway, Pyrimidine metabolism, CTP from UDP: step 2/2 pathway to avoid interference with native metabolic processes.4. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.5. The designed protein must possess ATP binding, CTP synthase activity, glutaminase activity, metal ion binding to support glutamine metabolic process, 'de novo' CTP biosynthetic process under certain conditions.6. The designed protein must have at least one CTP synthase N-terminal, Glutamine amidotransferase domain. The designed protein sequence is ",
    "response#1": "MQDAYKSIIESFIHAGAENDARVKLHWISAEDLETEKPETLLKGMYGILIPGGFGERGVEGKINAITFARENHIPFFGICLGMQCAVIEYARNVCGITKAQSSEFHKTRFNVIDIMPDQKNIKNIGGTMRLGAYPCIIEKNTKAYEAYKSEYITERHRHRYEVNNKFRAALQSEGLIFSGLSPNRELVEMIELPGHPWFVGCQFHPELKSRATKAHPLFRDFVEIMGAGANTILPDAYLSVTEAIRAGAFANQAKVNIKWVASDLCETEAGALEQLSDVDAICIPGGFGVRGIEGKLGALKFARENKIPTLGLCLGLQCMVIEYARNIVGLAGASSTEFDPDAKYPVIATMAEQVDILAAGEMGGTMRLGLYEAKLKPGSIVAEVYESELVSERHRHRYEVNNSFRDQIAAAGLVFSGTSPDDHLVEYVELPREVHPYYVATQAHPEFRSRPTKAHPLFKGLIAASLGRLS",
    "response#2": "KYVFVTGGVVSGLGKGITAASLGRLLKARGYKVTMQKFDPYINIDPGTMNPIQHGEVFVTDDGAETDLDLGHYERFIDESLNKNSNVTTGKIYWSILQKERRGDFGGGTVQVIPHITNEIKSRFYRNTTDDNIQIAIIEVGGTVGDIESQPFLEAIRQFQHDVGRDNAILIHVTLIPYLKASQEMKTKPTQASVKELQGMGIQPDIIVCRSEYPLDKQIKEKIALFCNVPSNHVLQNLDVEYLYEAPLAMEREHLAQVACESLKLDNPKPDLSRWEDMVNRIKSPKGEITIGLVGKYVELKDAYKSISEAIIHAGAKNSCKVNIEWLHAEKINTNNVKQKLSKLKGIIVAPGFGDRGIEGKITAIKYIRENNIPFFGICLGMQSAVIEYARNVLGLRNANSTEMDSKTSXPVIDLMNEQKEVTKKGGTMRLGTYDCYLKNNSNAYNAYKNNNIKERHRHRFEFNNKYKNDLEKAGLIATGINKNKNLVEIIEIAQHPWFVGVQFHPEFKSTVDNPHPLFCGFIKAAIKCKK",
    "response#3": "KYVFVTGGVVSGLGKGITAASLGRLLKARGYKVTMQKFDPYINIDPGTMNPIQHGEVFVTDDGAETDLDLGHYERFIDESLNKNSNVTTGKIYWSILQKERRGDFGGGTVQVIPHITNEIKSRFYRNTTDDNIQIAIIEVGGTVGDIESQPFLEAIRQFQHDVGRDNAILIHVTLIPYLKASQEMKTKPTQASVKELQGMGIQPDIIVCRSEYPLDKQIKEKIALFCNVPSNHVLQNLDVEYLYEAPLAMEREHLAQVACESLKLGLNNFKKNPNWENWLKDAYKSISEAIIHAGAKNSCKVNIEWLHAEKINTNNVKQKLSKLKGIIVAPGFGDRGIEGKITAIKYIRENNIPFFGICLGMQSAVIEYARNVLGLRNANSTEMDSKTSXPVIDLMNEQKEVTKKGGTMRLGTYDCYLKNNSNAYNAYKNNNIKERHRHRFEFNNKYKNDLEKAGLIATGINKNKNLVEIIEIAQHPWFVGVQFHPEFKSTVDNPHPLFCGFIKSIF"
  },
  {
    "reference": "MLPFCFEMTSMSYIGRFAPSPSGPLHFGSLIAALGSYFQAKSQHGQWLVRIEDLDPPREMPGAADLILKTLETYHLFWDGEVVYQSQRHHLYQAQIDHWLQSGQAYYCQCSRKQIKEMGGYYNGHCQELHLDAGAIRLKMTQPITHFDDLRHGQMHIPLELAQEDFIIKRRDGLFAYNLAVVLDDIDQGVTEVVRGADLIEPTGRQISLYHMLGQVPVRYLHLPLAMDKNGNKLSKQNHATGIDLTHPASMILEAMAFLGFAIPKELHQANLDEILRWGVQNWRLNQLPESLEITARFSNGTA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.2. The protein must have high specificity towards aminoacyl-tRNA ligase activity, ATP binding. The designed protein sequence is ",
    "response#1": "NSELVGKYGNFVNRVLAFAVKNGCTELSSPQLEQKDLDFISKSQKLAKDAAEHYAQYSLRKACSTIMELAALGNGYFNDEAPWKLAKEGNWNRVRAILFCACYCQKLLALISYPIMPETALKILEML",
    "response#2": "MTEELYLKDSYIKEFEGRVVRIEGNYVILDKTAFYPGGGGLDNDTGFLVNEKGERISVTEVKRGENGEILHKIDQNGSLNVNEKVIGTIDWDRRYRMMRLHTASHIVAALAYRKFGALITGGHISPEQAKDDFNVENKDTLIELINEANEIIKKDIELKIYFLPREEALMIPAIVKLAGRNPPQIPIWRIVEIPGIDIQADGGPHVKNTKEIGEIVLLKVENKGKGRKRVYYTVKK",
    "response#3": "NSELVGKYGNFVNRVLAFAVKNGCTELSSPQLEQKDLDFISKSQKLAKDAAEHYAQYSLRKACSTIMELAALGNGYFNDEAPWKLAKEGNWNRVRAILFCACYCQKLLALISYPIMPETALKILEML"
  },
  {
    "reference": "MSRVCQVTGKRPITGNNVSHSKRRTKRRFLPNLHWHRFWVEGENRYIRLRVSSKGMRIIDKKGIESVLAEIRANGEKV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.2. The protein design must be focused on enhancing the efficiency of translation. The designed protein sequence is ",
    "response#1": "KRVEIKPYVMEDQHCDECQGVLCKHNYAPYFCGDSSCLQYYCEACWDRMHYIVCDSRADHRPMVRL",
    "response#2": "KRVEIKPYVMEDQHCDECQGVLCKHNYAPYFCGDSSCLQYYCEACWDRMHYIVCDSRADHRPMVRH",
    "response#3": "KRVEIKPYVMEDQHCDECQGVLCKHNYAPYFCGDSSCLQYYCEACWDRMHYIVCDSRADHRPMVRL"
  },
  {
    "reference": "MIFENTLAFAREQDKLDPLAPYRQQFHHPVIDGQQVLYFTGNSLGLQPKSARDYVNSELDEWAKWGVEGHFNGKNPWVSYHEILTPASAELVGANESEVVCMNSLTTNLHLLFVSFYQPDGKRFKIISEAKMFPSDRYLLETQVRHHGFDPDEAIIEIAPREGEFLIREQDILDAIEEHRDELALVFFGGVNYFTGQLFDMKTLTRAAHKAGALAGFDLAHAAGNVPLSLHDWDVDFAAWCSYKYLNGSAGNVAAIFVHDRHGNNTELKRFGGWWGHDKERRFLMENHFQPMTGAEGWQISNAPVMGMAILKASLDIFAEAGMAALREKSLKLTAYLEYIFNQVVAQFPGYRLQIITPTDPQKRGCQLSIKLIGSDKKFFEALTKAGVIADFREPDVIRLSPTPLYNSFEDVYRFGETLQALLAQRGH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be optimized for kynureninase activity activity in order to improve 'de novo' NAD biosynthetic process from tryptophan.2. The protein design should take into account the physiological conditions of the quinolinate from L-kynurenine: step 2/3 pathway.3. The protein should have a low Km value for H2O + L-kynurenine = anthranilate + H(+) + L-alanine. The designed protein sequence is ",
    "response#1": "NSPLTQWQDMAEDCAKKSCDLVGASAHEIVIMNTLTINLHVMMASFYKPDSKRHKIILEWKPFPSDHYAIESQIKWHGLDPEKSMIKIEPDENSIIPTEKILRTIDEHAEETALLLLPGIQYYSGQLFDMPKITAYAKERGIIVGWDLAHAAGNVELKLHDWDVDFACWCTYKYINAGPGAIAGAYVHY",
    "response#2": "NSPLTQWQDMAEDCAKKSCDLVGASAHEIVIMNTLTINLHVMMASFYKPDSKRHKIILEWKPFPSDHYAIESQIKWHGLDPEKSMIKIEPDENSIIPTEKILRTIDEHAEETALLLLPGIQYYSGQLFDMPKITAYAKERGIIVGWDLAHAAGNVELKLHDWDVDFACWCTYKYINAGPGAIAGAYVHP",
    "response#3": "ASLVGARAHEVVVMNTLTVNLHVLMAAFYRPTAQRYKVILEARPFPSDHYAIESHVSWRGFDAKDAMVHICPDDGSEAGGVISTERILKLIDDHAHDAALILLPGIQYYSGQLFDMALITAHAHKRGLVVGWDLAHAAGNVELRLHDWDVDFACWCTYKYMNAGR"
  },
  {
    "reference": "MKDDDRLEDSGVSRRKLLTGSGKAAILAGLASAAAGGAVGIAVLPRPAQAKEATSEIRPGDLDEYYGFSSSGQSGEVRIIGLPSMRELTRIPVFNRCSATGWGITNESRKIMTEGLMPTTQAFLAKRGGVYPNGDLHHPHMSFTDGTYDGRYLFVNDKANCRVGRIRCDVMKCDKIVEVPNASDIHGLRVQKFPRTGYVFANSEHIIPIPNDGKVLDDPKQYWSVFTAIDGDTMKVAWQVLVDGNLDNTDADYQGKYAFSTCYNSERGVTLADMTSADRDWVVLFNLKRIEDAVKKGDYKEINGVPVVDGRHGSRYTRYVPAGNNPHGCNTAPDGIHVAIAGKLSPTVTLLDVRRFDDLFDDKIKPRDVVVAEPELGLGPLHTAFDGKGNAYTTLFLDSQVVKWNLDKAIQAYGGAKVDPIIQKLDVHYQPGHNHTSMGETKEADGKWLVSLNKFSKDRFLPVGPLHPENDQLIDISGDQMRLVHDGPSFAEPHDICLVHRSKVRPETVWKRDDPMWEEARQQAAKDGVKLEEAADVIRDGNKVRVYMHSVAPAYSLDKFTVKQGDEVTITVTNMDDVEDLTHGFTLVRHGIAMEIGPQATASVTFIADRPGVHWYYCQWFCHALHMEMAGRMIVEPQPA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "RPGSWGGLVLRVGQALFAAACIGVMGSSLGFASYTAFCYLIASMGLQMLWSFGLACLDGYAIRANKDLTSPILLSLFVVGDWVTAILSFAASSSAAGVVILFQKDVLFCRRYPQLPCGKYELATAFAFLSWAA",
    "response#2": "RPGSWGGLVLRVGQALFAAACIGVMGSSLGFASYTAFCYLIASMGLQMLWSFGLACLDGYAIRANKDLTSPILLSLFVVGDWVTAILSFAASSSAAGVVILFQKDVLFCRRYPQLPCGKYELATAFAFLSWAL",
    "response#3": "RPGSWGGLVLRVGQALFAAACIGVMGSSLGFASYTAFCYLIASMGLQMLWSFGLACLDGYAIRANKDLTSPILLSLFVVGDWVTAILSFAASSSAAGVVILFQKDVLFCRRYPQLPCGKYELATAFAFLSWAF"
  },
  {
    "reference": "MMFPINVLLYKWLIFAVTFLWSCKILLRKLLGINITWINLFKLEICGLSLEDGTVRLKSVRFAVFERKLFIKGLRIDSKKSSTNDLHKELPREEERTFIETPEDNGGGFISKILSLSQYWLNGVTIILEDTQLVNNDITIEKFGFFLSIDNSKHIKSLRFDSFLRKLLWNGQTIIADAIFIVNTNLLIGEIMNPLKDGLQVGLDLKLGDLNIPMNLLNLFINKENVDLMSNEKLLQRLADTTKANEELKDEDIAKMKDDLVYAMEKFVDRIKPLKEMNVTVDKLQIKDFPLTNHPELLGMNKYISYNVLVSNINFNTNRFRNEMPGYTLIFEERDSPFKFSIIMARFNIYLNLNRKHQSHAKQLKIIEIPNVSIFGETNLFSQKFRLSNNLHAKELENAIFNIKGNISSLTIDMDPVNISFIKCFLSNIKVFTSSCPKNKILKENSHVKFLTRRRVLFDYFKCFLPLINMKFTLDDPKFVINDKDDLIIGKFSVFMISHHSKRYTLGNNLMEEKEETQHIFYESHWNVELLDMKLQHIIKHQKYEHTILRVDSIAIEEKVQLLPDILCSANADIDTLMLDLSELPTMVMLSELVHNLDSQLANVEENYFKEFYEKFASNLQNMKAECSNMAKCLRQKEILPSDFMFQQLPDFFDYIKINIRDISSTLGARSVFMPRDVFSSVDSQSSKDLIDGKLRKYCNTVEKLQIALFGDKTQWHNKIGSNHATMVRSGQLTNFSKDNKQNPNHKSSIADLDDISTSDATEVNHLW",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be expressed specifically in the endoplasmic reticulum-plasma membrane contact site, mitochondrion, mitochondrial membrane, endoplasmic reticulum membrane2. Include a signal peptide as an essential component of the protein.3. For general function, the protein need meet that Tube-forming lipid transport protein which binds to phosphatidylinositols and affects phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2) distribution. The designed protein sequence is ",
    "response#1": "LYGFIRLKIYKYVVLVTSCDLHAAILGNNIYRARKFAIFPITRTLPFSTGLLNIKDEEELHYISLLNKHLSKGQILFSPTLDLTCSLQRLRVLTQSFELTSKYNYRFFWNKYAFHELIELTNKDLGFQEWIQPMIQGNIAITNSFLKTYNLRLCVITRHSPDYAGTRYFTRGVNAQGSAANFNEIEQIIMIESPITLEEQMVLSFTQIRGSIPMFWAEVNDLHYRPLLSLQPLDYSETVFGKHFQELANDYGDNLVVVNLLDQKGREAPLRSGFEKLCKRNKNPPLSYVYYDYHKL",
    "response#2": "DVEKAEWLNKIVAQVWPFLGQYMEKLLAETVAPAVRGSNPHLQTFTFTRVELGEKPLRIIGVKVHPGQRKEQILLDLNISYVGDVQIDVEVKKYFCKAGVKGMQLHGVLRVILEPLIGDLPFVGAVSMFFIRRPTLDINWTGMTNLLDIPGLSSLSDTMIMDSIAAFLVLPNRLLVPLN",
    "response#3": "LYGFIRLKIYKYVVLVTSCDLHAAILGNNIYRARKFAIFPITRTLPFSTGLLNIKDEEELHYISLLNKHLSKGQILFSPTLDLTCSLQRLRVLTQSFELTSKYNYRFFWNKYAFHELIELTNKDLGFQEWIQPMIQGNIAITNSFLKTYNLRLCVITRHSPDYAGTRYFTRGVNAQGSAANFNEIEQIIMIESPITLEEQMVLSFTQIRGSIPMFWAEVNDLHYRPLLSLQPLDYSETVFGKHFQELANDYGDNLVVVNLLDQKGREAPLRSGFEKLCKRNKNPPLSYVYYDYHKC"
  },
  {
    "reference": "MRHYYCAQQIRAAEAPLLAALPDGALMRRAAWPLAVAIAAELRRRTGAVAGRRVCAVVGSGDNGGDALWAATFLRRRGAAADAVLLNPDRAHRAALAAFRAAGGRIVESILPATDLVIDGVVGIGATGPLRPAAAELFAANTAPVVAVDTPSGVHVETGEITGPAVRPALTVTFGGLKPVHALADCGRVELVDIGLELPESDLRGMQAADVAARWPVPGRADDKYTQGVTGVLAGSATYPGAAILCAGAAVASHSGMVRYAGPAAAEVVSHWPETIAVADHRAAGRVQAWVVGPGLGTGHDASAVLRFALATDLPVLVDADALTVLAAHPELVADRAAPTLLTPHAGEFARLAGRPPGPDRLGAARALAARLGATVLLKGNVTVIAGPDGPAYLNPAGNSWAATAGSGDVLSGVIGALLAAGLPVAEAAAAGAFVHSAAADLAAKDPGPAAVPISATRILGHLRAALATIL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..2. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.3. The designed protein should contain one or more YjeF N-terminal domains that are essential for its function.4. A protein with ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding, NADPHX epimerase activity is needed to enhance nicotinamide nucleotide metabolic process in a specific context.5. The designed protein should have a high specificity for AMP binding, with minimal off-target effects.6. We need a protein that can perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate with high efficiency. The designed protein sequence is ",
    "response#1": "MGVLDNNAEWLGIPKGYLMECAGYSFATEVYLRYNLSNKKNSHALIFCGTGNNGGDGFVIARHLASLGIESTVVLAGNPQKITTKEAKTSWNIISNHLTNTIRINIAKDSTDIVDLGIILDQTGYYNIIIDGLLGTGIKGKIREPIATAIDFINEAKTHKNIPIISIDVPSGLNPDTGEVSDKAVKADLVITFHKTKIGMKEGREFINEIVVRSIGIPQEAEEPSDIGAMLPARSVDAHKRSTGSVLVLAGSDGMSGAALLTSRGAARMGAGYVTLVSTSYVDHAKKSLIPEAVSRIVADHSELGPEVLAEFDDDFDRADAVALGPGLGTGPRQQALVETALEAIDVPLVLDADGLNVLAEDAAALRKRTSPTVLTPHPGEMATLLKLSSKDVQADRVGVARRAASDLGCVVVLKGAGTIVAGPEGPAVVNPTGGPELATAGTGDVLTGVVGALLAEGLDPVSAAIAGVFVHGLAGGVAGVSAGGRGTVAWDVAEALPAAVGELYERDAHKYARGLLAVIGGAMPGAGVLASRAAMHGGAGYVKLYSDHDGLPGVPPDLVVAGDPMPALATDPRIAAVLIGPGLGRSEEARSVLSWVLACDLPTVCDADALVLLEPEMLEDRSAPLVLTPHAGELDAIGNAFASSEPDRLEQVTELAEAIEGVLVAKGPDTIVAAPGRPPVFMPPAPSWLSTAGTGDVLAGLIASQLAVGETSAFQAAQNGCWLHREAALRAGPAFSASGLVR",
    "response#2": "MRAIEINSEALGIPTLLLMENAGRSVKDEILARIKPNNAVIFVGHGGKGGDGLVTARHLAGEGVKVKVIILGENKHRDAVFNYNAILDMDYSIEINEIKDLEELKPVEADVLVDAMLGTGFRGKPREPFATAIRVFNESKGFKVSIDLPSGIDADTGEAPGEYVKPDLVVTFHDMKKGLLRYNFNVIVKKIGIPPEAEVTIFAGSNGYSGAAYIATEAAVKCGSGLVTLCTAEELQAILSSKLVEAMTVSVDNKNRIEELISKSDAVAVGPGMGNTKETFKTLESIISKVSCPIVIDADGLNVLKDKLNILKLKKNKIILTPHPGEFSRISGITVKDIEQDRINISKKFARENDVILVLKGYHTVVTDGESVYVNSTGNSAMASGGMGDCLTGMIVSLIGQGYSPIMAAVCGTFIHGYCGDILSEKMSNVTASDIIYAIKRKEEDNKTTYGKTYIVAGSKNYYGAAELATKSCVKCGSGYTYLLSDYSEIENIVKNIPEAIYEKEIDNLNKSTTIAIGPGMIFNEFIYNIIEEYKDDKDIIIDAGALNNNINFNNAEKTLLTPHTGEFSKISNIDLEHILKNPIEVVLDYSRKNKVTLLLKGKNTYISDGEEVYIIDTGNPYMANAGMGDVLTGMIASLNSQGYNLKKSAIIATYLHGYIADKLREKQYIINPTDIIENISKYME",
    "response#3": "MAAADEATIASGTPGFVLMERAGRAVARATVTLAGRRYGARVAIVCGKGNNGGDGFVAARVLHREGVAVRCLLLAEESEYEGDAAAHLRAMSRAGVKPRPFETHSLSYHDVVVDAIFGTGFRGRAEGRPAKAIEAINSNGARVLAVDIPSGVAGDTGAVEGPAVRANVTVAIAAQKLGTVLHPGAGRAGRVEVVDIGIELGDAEPSDIGAMLPARSVDAHKRSTGSVLVLAGSDGMSGAALLTSRGAARMGAGYVTLVSTSYVDHAKKSLIPEAVSRIVADHSELGPEVLAEFDDDFDRADAVALGPGLGTGPRQQALVETALEAIDVPLVLDADGLNVLAEDAAALRKRTSPTVLTPHPGEMATLLKLSSKDVQADRVGVARRAASDLGCVVVLKGAGTIVAGPEGPAVVNPTGGPELATAGTGDVLTGVVGALLAEGLDPVSAAIAGVFVHGLAGGVAGVSAGGRGTVAWDVAEALPAAVGELYAESDKYRRGVLGVAAGSHLYTGAAVLAVGGALRAGAGMVRYAGAEEPVAQVRAHWPEAVITVLDRPSIDEVGRVQAWVLGPGLGTGERAHDLAASVLASDVPVLIDADGLTIVAKDRELLRRTAPVLLTPHAGELARLTGAERADIEAARLEHVRAAAAELGVTVLLKGSTTLIAGPSGQARVNVTGSSWLATGGTGDVLSGVAGALLAQGLSCYDAAACGAYVHGLAGL"
  },
  {
    "reference": "MMSHIRLVLCSALLSMMSCPTSGTIGEMLSLAASSNSSICHGMQFTEPTESFMGILPNETLPMFIFSIMHSEAVPKVGKTLRISHDMKLFGGEAVNYMIFVNKILYEPISYYNYTGSCRQQGLSSCVRVYSDLVNGSKGNPSVKLGFTAIELNKTKNTDFHNLGFKICFSCRDHNGIRLVVYNKNNRTAQLMMCPSELIFSQDFTINSTSVNPSEEAAVPLLNVTGYTCIALHNKNMLTHHSPALSSGSKVDNTLEPGCDSNVGLFGHSTGTDYGWGLANFFSAGITNSLQISQLEHVTDAIACKIAKTSNYTTTALFLLNKEEGEIRDHVIEHEVALNYLLAHQGGLCSVVKGPMCCSDIDDFRRNVSDMIDKVHEEMKKFYHEPDPFGGLGTWGFYGTIFGHVLQWIPIIIMVVVVCFVCSWVRK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The Cytoplasmic, Extracellular topological domain of the protein should be designed to be flexible or rigid.3. The protein must exhibit the following characteristics: mediates virus attachment to host receptor and fusion of viral and host membrane..4. The protein should be designed to localize specifically to virion membrane, viral envelope, membrane, host cell plasma membrane in order to perform its role in fusion of virus membrane with host endosome membrane, viral entry into host cell, virion attachment to host cell more effectively. The designed protein sequence is ",
    "response#1": "MGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNTSWSNKSYSQIWDNMTWMEWEREIDNYTKTIYTLIEESQNQQEQNEKDLLELDQWASLWNWFTITKWLWYIKIFIMIVGGLIGLRIVFAVLSIVNRVRQGYSPLSFQTY",
    "response#2": "ILLTQIQFAYDTLRAQINNVLEELSRAWCREQHRASLMWNELSKINPTSVMSSIYGRPVSAKRIGDVISVSHCVVVDQDSVSLHRSMRVPGRDKTHECYSRPPVTFKFINDSHLYKGQLGVNNEILLTTTAVEICHENTEHYFQGGNNMYFYKNYRHVKTMPVGDVATLDTFMVLNLTLVENIDFQVIELYSREEKRMSTAFDIETMFREYNYYTQRVTGC",
    "response#3": "TINSTRIKNSEEIKNCSFNTTTELRDRRKKEYALFYRLDITPLDNKKNSSENSSEYYRLINCNTSAITQACPKVTFDPIPIHYCAPAGYALLKCNNKTFNGTGPCNNVSTVLCTHGIKPVVSTQLLLNGSLAEGEIIIRSENLTNNAKTIIVQLNQSVEIVCTRPNNNTRKSIRIGPGQTFYATGDIIGDIREAHCNISADKWKETLQRVSKKLREHFPGKTINFTSSSGGDLEITTHSFNCRGEFFYCNTSGLFNGTYNPNVTESNSNSTNATITLPCRIKQIINLWQEVGRAMYAPPIAGNITCKSNITGLLLTRDGGTNDTSKTEIFRPGGGDMRDNWRSELYKYKVVEVKPLGVAPTGAKRRVVEREKR"
  },
  {
    "reference": "MGSKLSKSKIVGWPAIRERMRRTNPAAERVRTNQAAEGVGAVSRDLEKHGAVTSSNTAHTNPDCAWLQAQEEDEEVGFPVKPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIWSQERQDILDLWVYHTQGYFPDWQNYTPGPGIRYPXTFGWCFQLVPVDPEEVEENNEGENNCLLHPMAQHGMEDKEKEVLKWRFNSRLAFEHKAREKHPEYYKDC",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK12. The protein design must feature the Dileucine internalization motif; necessary for CD4 internalization motif.3. The protein should be optimized for stability and expression in extracellular region where it will participate in suppression by virus of host autophagy. The designed protein sequence is ",
    "response#1": "LSSG",
    "response#2": "GFSS",
    "response#3": "APS"
  },
  {
    "reference": "MLTGKRILLIVGGGIAAYKSLELIRRLRERGAAITPVMTAAAAQFVTPLSVSALAGETVHGDLFDLTAEAEMGHIQLSRVADLVVVCPATADLMAKMAQGLAGDLASTLLLATDTPVMIVPAMNVRMWDHPATQRNLATLRGDGVTVVGPDNGDMACGEYGPGRLAEVPQIIDAVQRALSDGPLTGKRVVVTSGPTHEPIDPVRYIANRSSGAQGTAIAQALRDLGAEVVFVTGPADVTRPTGVEVIEVQSAVQMRAAVMAALPADAGVFAAAVADWRVENAGGSKIKKVAGQMPTFRFAENPDILAEVSKLPEGRPRLVVGFAAETDDVVAHATAKRLRKGCDWIVANDVSPATGIMGGTENAVVLISDSGAEAWPRMGKREVAGQLARRIAAALDESAG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein is the optimization of the Proton donor to achieve high catalytic efficiency for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.2. The protein design should take into account the physiological conditions of the coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway.3. The protein must exhibit metal ion binding, FMN binding.4. The protein should be able to bind CTP ligand in a variety of conditions5. The presence of Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains is critical for the interaction of the designed protein with its intended targets.6. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine. The designed protein sequence is ",
    "response#1": "KTIVIGVAGGIAIYKIADLCSKLTQKGADVWVLMTEAATKFVQPVTFQALVRNPVLTDIFQEPNPSVISHIDVADRADLFLIAPATANIIGKMANGIADDIVTTAYLATKAPVWIAPAMNVNMYNHPAVQHNIRRLAEYGYRFIEPAEGYLACGWIGKGRLAEPMDILQEMLTWFSAHPQPLAGKRVLITAGPTRERIDPVRYISNHSSGKMGYALAEAARDRGADVVLVSGPTDLPRPDGVTFVPVESVQEMYDAVLDRFEASDIVVKAAAVSDYRPVSTHSHKMKKTTGTLRLELEKAPDILQTIGRQKTHQYLVGFAAETQDLLRYAQEKLVRKNADMIVANDVLAEGAGMGVDTNIVTLITADSVERLPLMPKAEVAERIVDRVVALLQQSPS",
    "response#2": "KHILLIIGGGIAAFKSLDLIRRLRERGAIVTPVLTRAGEEFVTPLSVAAIAGQKLYRDLFDLGDEAEMGHIQLSRVADLIVVAPATADLMAKMAGGLANDLASTLLLATDTPVLIVPAMNVRMWDHPATQRNLKTLRGDGINVIGPNDGDMACGEFGPGRMSEPLEIVAAIERALARAAALRGRHVVVTAGGTREPLDPVRFLGNRSSGKQGYALAKAAAARGARTTLIAANVTLPDPAGVELIRVGTTEELRAATVKAAAEADVIVMAAAPADFRPVTVAAEKIKKTDSGTPAPIELTTNPDIAAELGAAKREGQVLVAFAAETHDALENARAKLIKKRADLIVVNEVGVDRVFGADHNEVTLIRADGAAESPAGSKRAVAAAIWDQVQHLL",
    "response#3": "EVVLGVGGGIAAYKACELLRLFTESGHGVRVVPTASALQFVGAPTWAALSGRPVATEVWDDVHEVPHVRIGRAAELVVVAPATADLLAKAAHGIADDLLTNTLLTATCPIVYAPAMHTEMWENPATRANVATLRARGAVVIEPAVGRGPGRMAEPERLAAVALGVTGGIGAYKAVEIARGLQKRGHDVVAIMSHGALKFVGEVTFEAITKRRVITDQYAPGANADIEHISLATDIGLLLVAPATANVIGKFAHGIADDFLTSLYLATRAPVLMAPAMNTNMLEHDAVSRNLATLTARGVRFVDPGQGYLACGWVGKGRLAEPDDVVEAALRLLGPGERDLLRGRHVVVTAGGTREPLDPVRFLGNRSSGKQGYALAKAAAARGARTTLIAANVTLPDPAGVELIRVGTTEELRAATVKAAAEADVIVMAAAPADFRPVTVAAEKIKKTDSGTPAPIELTTNPDIAAELGAAKREGQVLVAFAAETHDALENARAKLIKKRADLIVVNEVGVDRVFGADHNEVTLLGADGTRTTVDGPKTGVAAAVLDLVATLLR"
  },
  {
    "reference": "MNTVSNSLNELAPVLGAAAMRQADRVTIEEIGIPGFTLMESAGRETTRVAQEMIELLMDVYSQADSPATGHVICVCGKGNNGGDGFVIARYLASYGHRIEVLLMAPESGYTNDAAANLKILRKLAAIQKNLTVLPFSTTAALDKLETPDLLVDALLGTGLQSAVRAPYDAVIGWMNALMVPVLSVDVPSGISSDSGHMMGCAVAAAETVTMGALKPGLLLGDGPDYSGALHVVDIGIPAHVLQRIAASHDSPRISNSRYVAARLPEKSRKDHKYTSGPALIAGGSASFPGAPALAARGAARSGSGYVVCACPEEIRTILLEKLDDIPVESWPDNAQNGADAVQRLVTKLGARWTKAKSLLVGPGLGRAEGAPDLVKALLTSFNGPCVVDADALFALMEERDWVRAHSGGQWVFTPHVGEFHRLLGPSSEIDLLKMGIQFAKEWNVILLLKGQPSVTFSPDGHQVINTTGNPGAATAGSGDVLAGVVCGLLSQGLSPLDAAIGGIHLAGTAADLFVEERAAPSMVASDILELLPSAIAQLLPSE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.2. The protein should have a fast turnover rate for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.3. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.4. The designed protein must be optimized for ADP-dependent NAD(P)H-hydrate dehydratase activity, metal ion binding.5. The designed protein should be able to compete with endogenous proteins or inhibitors for K(+) binding. The designed protein sequence is ",
    "response#1": "MRQLDSETIQQLGIPAIALMENAGRVIAEEIITLCRERGGAGCRNAAERNDTGLREAALGGTGKAFKVSADHALTLASAAAERWFILVGKGNNGGDGLAAARYLRETGIAVTLVYAVPPESLSGEAALQRDAAAAMGLPAVVYGRDRLDLAECSGILDALLGTGGAGAPRGAYAELIAAANSSGKVIVSADIPSGLNADTGETHEPCIHASVTVCLAFLKRGLLQYPGAGAAGHVKVRAIGIPTALAEPEEIKTALPPRDPDAHKGTYGHVLVLAGSPGKTGAAALCSLAALRAGAGLVTLAVPEGLNDLMEVKLTEVMTVGLPETEERTVAFQARDALLGLMEGKRVLALGPGLSTHPETVRLVAALIQAAKIPLVIDADGITALARQPEVLSKASVPVILTPHPGELGRLLSVPKEEVIEKQIPIAQKVTSTYNVTLVLKGARTLIAEPGGAVHINPSGNPGMATAGVGDVLTGVIAGLISQGLAPAIGAVVGVYLHGLAGDLACRELGPEAMIASDLLEKLPEAIRAFKSV",
    "response#2": "MRQLDSETIQQLGIPAIALMENAGRVIAEEIITLCRERGGAGCRNAAERNDTGLREAALGGTGKAFKVSADHALTLASAAAERWFILVGKGNNGGDGLAAARYLRETGIAVTLVYAVPPESLSGEAALQRDAAAAMGLPAVVYGRDRLDLAECSGILDALLGTGGAGAPRGAYAELIAAANSSGKVIVSADIPSGLNADTGETHEPCIHASVTVCLAFLKRGLLQYPGAGAAGHVKVRAIGIPTALAGAGKVSLATSPEHVAASLVRCPEVMAHGVRGGAEASMLPSSDVVVVGPGLGQDAWGQAILQSALQSELPLVVDADALNLLASRWSEVRRDNWVLTPHPGEAARLLGCSVADVQADRPAAARALQRERGGVVILKGAGSLVAGPSGLVVCPYGNPGMASGGMGDVLSGMLGTLIAQNDSLELAAWLGVIVHAQAGDDAAKNAGERGLLASDLL",
    "response#3": "MRAAEAAAFAGGVKQDALMERAGLAIAREVRRVALGRPVLILAGPGNNGGDAFVTATILRGWGLDVTVAALPGRAGGAAGRMRAAWGRPVEEVDTVQPHPILVDGLFGIGLSRPLDPAIAARLTLLASDALTIAIDVPSGIDSDAVHDFASAGTADLTVALGALKPAHVVGDAVARCGHVLLADLGLTLPAAVAVLGGAMPGAARLAARAALSAGAGYVILAGEAGPGGPDALVHRPPDDLTALLADDRLAAVLVGPGLGRDAAATRKLDAALACAAPLVIDGDALSLLGPAGIPRIAARSAPTWLTPHGGEFSRMFGTGDGNKIDRTVHAALQARATIVHKGPDTVIASPDGTVTVAPHASSWLSTAGTGDVLAGILAARLRPGGADAARQAVWLHGRAARLAGPAFVADTLIDHLGQAHPALWLSRYPWPEINGNKYQRGHVLVLGGDTVTGASRLTACGAMRAGAGLVTLASPPDTWMIYASALTSAMVRPFNGLAGFTELLADARRNVIAIGPGANVGDATRQYVLAALAARRTTVLDADALTAFAETPRRLFDAIDGPCVLTPHEGEFDRLFTAGGDKLLRTRRAAVESGAVVLLKGADTVIAAPDGRAIVNANAPAHLATGGSGDVLTGFIAGLLAQGLDVFHAAAAAAWLHGEVANYLGIGLIAEDLPDALPHVLQALGV"
  },
  {
    "reference": "MTAQILDGKKAAAEVKSELVERIAALVARGITPGLATVLVGDDAPSQSYVRMKHQDANALGLASFERHLPAETSQDELDAVIDELNANPGVHGILVQIPLPKHLDEQAVLERIDPDKDADGLHPVNLGRLVLNVNGVITSPLPCTPVGAIQLMLRNGIELAGKHVVVVGRGITVGRALGLLLTRREINATVTLTHTGTVDLAAHLRQADVVISAVGVAHMIKAENLKPGVVVLDVGVSRERDEVTGKSKLLGDVAPDVADVASWISPNPGGVGPMTRALLMSNVVQAAEAVSSR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. We need a protein that can perform 5,10-methenyltetrahydrofolate + H2O = (6S)-10-   formyltetrahydrofolate + H(+) with high efficiency.2. The designed protein should be optimized for methenyltetrahydrofolate cyclohydrolase activity, methylenetetrahydrofolate dehydrogenase (NADP+) activity activity in order to improve purine nucleotide biosynthetic process, tetrahydrofolate interconversion.3. A protein that is capable of catalyzing reactions within the One-carbon metabolism pathway with high efficiency and specificity.4. The protein must exhibit the following characteristics: Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate.. The designed protein sequence is ",
    "response#1": "MSGKPLHEKTKEWARRHVRLLEEVGVTPKLAVLLLNDDPVELETQRRYVSLKARDVREVGGEVEIYELVDVPPERRSKEALYLIERLNRRDDVTGILIQKPVPPFVDEDLLFQRLSPEKDVDGLCIDKPGGVGPMTPFCLGRLLIDSPLFIPCTPKGIIRMLDEYKINLEGKQAVVIGRSIIVGKPLSLLLLKRNATVTMCHSKTINLQDITKKADILCVAIGREKFITSNMIKKGAVVIDIGINVTASGKVVGDVNFDDVKEKASYITPVPGGVGPMTIAMLMENAIYAAKLPEKDVDGFHVANAGALMVGQSEFKPCTPYGCMKILESIEYPIRSARAVIVGASNIVGKPMAMLLLQAGATVTICNSKTRDLAHHTKDADILVVATGKPKMISGDMVKNGAVVIDVGINRLPDGKLCGDVDFDTAKYVAGWITPVPGGVGPMTITMLLMNTLEAAS",
    "response#2": "MSGKPLHEKTKEWARRHVRLLEEVGVTPKLAVLLLNDDPVELETQRRYVSLKARDVREVGGEVEIYELVDVPPERRSKEALYLIERLNRRDDVTGILIQKPVPPFVDEDLLFQRLSPEKDVDGLSQLNAAFNPAKDVDGFHVVNAGRLAVGLDALVPCTPLGCVMLLKHHLGNLSGLNAVVVGRSNIVGKPAAQLLLREDCTVTIAHSRTRDLPGMCRQADILVAAVGRPEMVRGDWIKPGATVIDVGINRVPKADGKTRLVGDVAYEEALGVAGLITPVPGGVGPMTIACLLQNTLTAAYNLASNYNCKDNFLSATPKGIILLLKHYNINFRSTVIALVGQSNIVGKPLAKYLSNFKNNTIKTYVKDTPKDDLHEAKIVIVATGVYQSVTADQLANGTVLIDVGIHRQGKTIHGDLDFASCFKKASYITPVPGGVGPLTVVALMFNLIKAYNLASNYNCKDNFLSATPKGIILLLKHYNINFRSTVIALVGQSNIVGKPLAKYLSNFKNNTIKTYVKDTPKDDLHEAKIVIVATGVYQSVTADQLANGTVLIDVGIHRQGKTIHGDLDFASCFKKASYITPVPGGVGPLTVVALMFNLIKAYNLASNYNCKDNFLSATPKGIILLLKHYNINFRSTVIALVGQSNIVGKPLAKYLSNFKNNTIKTYVKDTPKDDLHEAKIVIVATGVYQSVTADQLANGTVLIDVGIHRQGKTIHGDLDFASCFKKASYITPVPGGVGPLTVVALMFNLIKAQS",
    "response#3": "MSGKPLHEKTKEWARRHVRLLEEVGVTPKLAVLLLNDDPVELETQRRYVSLKARDVREVGGEVEIYELVDVPPERRSKEALYLIERLNRRDDVTGILIQKPVPPFVDEDLLFQRLSPEKDVDGVNLGYPEFPHKDVDGFHPYNIGRLAQRIPALRPCTPKGIMTMIESTKRPVKGLDAVIVGASNIVGRPMSLELLLAGCTVTTCHKFTQDLKSHVQRADLLVVAVGKPNFIPGDWVKPGAIVIDVGINRVNDGSLVGDVDFDKAKERAGWITPVPGGVGPMTVASLIENTLEAYNLASNYNCKDNFLSATPKGIILLLKHYNINFRSTVIALVGQSNIVGKPLAKYLSNFKNNTIKTYVKDTPKDDLHEAKIVIVATGVYQSVTADQLANGTVLIDVGIHRQGKTIHGDLDFASCFKKASYITPVPGGVGPLTVVALMFNLIKAYNLASNYNCKDNFLSATPKGIILLLKHYNINFRSTVIALVGQSNIVGKPLAKYLSNFKNNTIKTYVKDTPKDDLHEAKIVIVATGVYQSVTADQLANGTVLIDVGIHRQGKTIHGDLDFASCFKKASYITPVPGGVGPLTVVALMFNLIKAYNLASNYNCKDNFLSATPKGIILLLKHYNINFRSTVIALVGQSNIVGKPLAKYLSNFKNNTIKTYVKDTPKDDLHEAKIVIVATGVYQSVTADQLANGTVLIDVGIHRQGKTIHGDLDFASCFKKASYITPVPGGVGPLTVVALMFNLIKAYNLASNYNCKDNFLSATPKGIILLLKHYNINFRSTVIALVGQSNIVGKPLAKYLSNFKNNTIKTYVKDTPKDDLHEAKIVIVATGVYQSVTADQLANGTVLIDVGIHRQGKTIHGDLDFASCFKKASYITPVPGGVGPLTVVALMFNLIKA"
  },
  {
    "reference": "MRKASRSVGAAPKVPANNKSQATERSKSESSAPTASKVSRPGSSLSKAKSNDDLLAGMAGGLPASNSVKVKKNSTTSYPNSGTAMSGQEGRTRSSAGSSSNTKRSGSSGAKEVGSSRERLRERSRLTANKKPQGLGVGTGEVSAPSKRSRGRTDSDMIRMSKSKSDNQISDRAALEAKVKELMNLAKNKDAEILLLRTELRDTRSQLGQDVSDKSPDDLPLIHLQEQNTTVCEELQQLKSENRMLKDRLNALGFSLGQQPDDPDKLYGFQSLGINPGSHSDCGGGTLTSSVEGSAPGSMEDLLSQDESTLTGERRSSSMDNLDSECSEVYQPLTSSDDALDAPSSSSESEGLPSTERSRKGSSGNASEVSVACLTERIHQMEENQHSTAEELQATLQELADLQQITQELNSENERLGEEKVILMDSLCQQSDKLELFSRQLEYAQALLDEHHIAYSLDEDLKSSRYLDLEQRYMDLAENGRFEREQLLGVQQHLSNSLKMAEQDNKDAQDVIRALKERNHHMERIAEAEQLSKQALAATLEEYKATLNSEQGECARLKALLEQEKQRVAELYSIHSSGDASHIQNLLESVRSDKEKAESLASSLQEELLHARTDVNRMQDAFGKLEDEYRAFREEAQKQVSELTLALEKVRHELEEKETELSDMKETIFELEDEVEQHRAVKLHDNLIISDVENAVKKLQDQKHDMEREIKILNRKLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGELKRQLQEALEKNEKLAK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that May play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration .2. The designed protein's functionality will benefit from Polar residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "MCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLWPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNSLMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGR",
    "response#2": "MCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLWPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNSLMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGC",
    "response#3": "MCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLWPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNSLMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGC"
  },
  {
    "reference": "MDTDLSPQDRKDLDKFIKFFALKSVQVIVQARLGDKICTRSSSSPTGSDWFNLAIKDIPEVTHEAKKALSGQLPAVGRSMCVEISLKTSEGDSMELEVWCLEMNEKCDRDIKVSYTVYNRLSLLLKSLLAVTRVTPAYRLSRKQGHEYVILYRIYFGDVQLLGLKEGFQAVRVGTVGTPVGTLTLTCAYRTNLAFMSTRQYEWTQPIMGIIIDHFVDRPFPSTSHMHPCNYRGAEEQVLTYPAVEESQEVCTTSFSTSPPSQLCSSRLSYQPAVPGAGPSELGYPVALATGVSAGHPHQMMAPGKESGLPAVSVQPAHGNQAEHDRTLSVSPANVAGTTSSSEEADSPSGSSTTGAKSSPNKNTHVLFTRKVGAFVDKPSPQVTYASTDIPFAVFVPKLADNEDTDPMVVYPETPEEESPEARSLHSSCSSSGTPQDDFVMVDFKPAFSKDDLPMDVGTFYREFQNPPQLSSLSIDIAVQSMAEDLDSLPEKLAMHEKNMKEFDAFVDSL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have a specific function in autophagosome assembly and should be localized to Atg1/ULK1 kinase complex in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "IQNFHTKAAIIVLGARVGLPVVFTKEGTKKVNKWFQIETDDTDAYRDELHTWRMCGGFQNRPPPLIIETYLDTSHLTSSQRLVIVDDLGKRWDALDALSNSCGSNDGRSEVILERWKIELRDIPGEQPYDFGSTLPTIYKKCIVFFRSLYSTAKLVPAWKFKKSLGKNGSPTNSLIINCRITTGDTQPRRIDGLKQPILDHSGPVTSTYVLGATDTPAGQIFAEVTYRNDCNN",
    "response#2": "REFLRDVHSRIWLTYRSGFPLIKRAEDGPSPLSFGSLIRGTVDLATVTKGFTTDAGWGCMIRTSQSLLANSLLQLRLGRGWRYDQTRECAKHAEIVSWFVDIPTAPFSIHNFVEQGANCAGKKPGEWFGPSAAARSIQVLCEANYDKTGLKVYFTASGDIYEDELFELAQQGAELRPVLILAGIRLGVKNVNPLYWDFLKKTLGWPQSVGIAGGRPSSSHYFFGFQGDYLFYLDPHVPQKALLIASEAPHESPDPNHYVEVESGLDLDSVHTNKIRKLHLDQMDPSMLVGLLVENRASYDALKY",
    "response#3": "IQNFHTKAAIIVLGARVGLPVVFTKEGTKKVNKWFQIETDDTDAYRDELHTWRMCGGFQNRPPPLIIETYLDTSHLTSSQRLVIVDDLGKRWDALDALSNSCGSNDGRSEVILERWKIELRDIPGEQPYDFGSTLPTIYKKCIVFFRSLYSTAKLVPAWKFKKSLGKNGSPTNSLIINCRITTGDTQPRRIDGLKQPILDHSGPVTSTYVLGATDTPAGQIFAEVTYRNDCNR"
  },
  {
    "reference": "MNVLLGGLVIFATFVTLCNGSCYLMPNKMVPGDSTKECTDLKGNKHPLNSRWKTENCDECDCLEKEISCCSLVAIPVGYDQDNCQKIFKQEDCKYIVVEKKDPNKTCEVTQWI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have a specific function within the extracellular region. The designed protein sequence is ",
    "response#1": "MIS",
    "response#2": "PGDGGPPGQPGPDGPPGAPGNAGEDGKPGDQGPRGPPGIPGAPGQPGRPGPPGEPA",
    "response#3": "GRAGAPGRPGPPGQPGPAGPPGENGKGGGQGPSGLPGPPGQPGQNGAPGEVGQPGDNGC"
  },
  {
    "reference": "MENRWQVMIVWQVDRIRIRTWKSLVKHHMYISKKAKGWSYRHHYESTHPRISSEVHIPLGDSKLVVTTYWGLHTGERDWHLGQGVSIEWRKERYITQVDPDLADHLIHLYYFDCFSDSAIRKALVGLRVSPRCEYQAGHNKVGSLQYLALRALITPKKIKPPLPSIKKLTEDRWNEPRKIKGHRGSHSMSGQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions. The designed protein sequence is ",
    "response#1": "EAEECF",
    "response#2": "EPWE",
    "response#3": "PPEPC"
  },
  {
    "reference": "MTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVTIQKFDPYLNVDPGTMSPYQHGEVFVTHDGAETDLDLGHYERFIDINLNQYSNITTGKVYSSVIKKERRGDYLGGTVQVIPHITNEIKDQVFRAGEATKADVVITEIGGTVGDIESLPFLEAIRQIKNEVGRNSVMYIHCTLIPYIKAAGELKTKPTQHSVKELRSLGIQPDVIVLRTEQSVSKEMKEKISLFCDINERAVIEMPDADTLYQIPITLQEQGLDELTCNHFGLECGEPEMGEWKELISKVRNLSKTIDIGLVGKYVELPDAYLSVVESLKHAGYVYDADINVHWINSEKLTEEGIKEELDKVDGVIVPGGFGDRGIEGKISAIRYARENNKPFFGICLGMQLASVEFARNVLGLEGAHSAEIDPRTPHPIIDLLPEQKEIEDLGGTLRLGAYPCKLKEGTKTQAAYDGADVIEERHRHRYEFNNEYREQMEAEGFIFSGTSPDGRLVETIELKDHPWFVACQFHPEFTSRPTRAQSLFKGFIGASVEQKA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a high affinity for UTP, CTP, ATP, L-glutamine.2. The designed protein must have a active site, Nucleophile; for glutamine hydrolysis, Nucleophile active site that is highly conserved among related enzymes.3. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.4. The presence of Glutamine amidotransferase domains is critical for the interaction of the designed protein with its intended targets.5. A protein with metal ion binding, glutaminase activity functions is required to enable 'de novo' CTP biosynthetic process.6. A protein that can modulate the overall flux of metabolites through the Pyrimidine metabolism pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "RYIFVTGGVVSSLGKGITAASLGAILEARGLKVSMIKLDPYLNVDPGTMSPFQHGEVFVTADGTETDLDLGHYERFVRTTTGRNSNFTTGRIYGSVIAKERRGDYLGATVQVIPHITDEIKRCIRLGAGDADVCIVEIGGTVGDIESLPFLEAIRQLGVDLGPQNCVYVHLTLVPYIASSAEIKTKPTQHSVKELRSIGIQPDILVCRSEQPLPDEQRRKIALFTNVQERAVISAVDVDDLYKLPMALRSQGFDTIVVDRLGLAARKPDLAEWEKVVQRIQSPKRSITVGIVSKYGIEDVYLSVKEALKHACIASGCRVNIKWIESEDVEKASDPARFFNDVDGILVPGGFGNRGVEGNIKAIQYAREHNIPFLGLCFGFQLAVIEFTRHVAGFPDATSSELGPGTHVIDLLPEQKDVKKMGGTMRLGEHMVWVKDNTLAHRLYGKGKISERHRHRYEVNPDLIDHIEHHGLIFSAKNDNRMEMCELPGHKFFLGTQFHPEFKSRPERPAPPFLGFVAAALARPASAG",
    "response#2": "KYIFVTGGVVSSLGKGIAAASIGCLLEARGLRVTLLKFDPYINVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFTRARMSQDNNWTTGRIYLSVIEKERRGDYLGKTIQVIPHITDEIKQAIRNVSSGVDVVIVEIGGTVGDIESQPFLEAIRQLGNEVGRSNALFIHLTLVPYIPSAGELKTKPTQHSVKELRAIGIQPDILLCRTDRQLPPEIKSKIALFCNVSEEAVVAARDVEFVYEVPLEFAKQELDDLILERLQLEAGPADMTEWTVLVERIANPAHQKDAYKSIYEAIDHAGMHHQAQVRIGRVQSSDIEREGAERLLSGYDGILVPGGFGERGIEGKVQAIRFARERGIPFFGICLGMQCAVIEYGRDVCGLEKAHSSEFDKDTPHPVICLLDEQQNVTQMGGTMRLGSQPTQLLEGSRARQCYHADSVNERHRHRYEFNNDYRQQYRDHGMIFSGTSPDGGLVEIIELENHPWFVAVQYHPEFKSKPLKAHPLFAGFVRSAALRRARGLPDAYLSVTEALRAGGFAHRAKVEIRWVPSDDCETPAGAAAALDGVDGILIPGGFGIRGIEGKLGALTHARTRQIPALGLCLGLQCMVIEAARNLAGLVDASSSEFDPRTPHPVISTMAEQLDVVAGERDMGGTMRLGAYPAALGEGTVVAKAYGSREVSERHRHRYEVNNSYRARLAEAGLVFGGTSPDGTLVEFVELPAEQHPFYVGTQAHPELKSRPTRPHPLFAAFIDLRGGSE",
    "response#3": "MTKYIFVTGGVTSSLGKGIISASLAKLLQARGYSVTIQKLDPYINVDPGTLNPYEHGECYVTDDGAETDLDLGHYERFLNVFTSQANNVTTGKIYQSVINKERRGDYLGKTVQVIPHITDEIKRRILLLGSKHKFDVVITEIGGTVGDIESLPYIEAVRQLKWELGSNNTLYIHLTLVPFLSASAELKTKPTQHSVKELLENGIQPDILVLRTEHELSDDIRKKVALFCNVDRDAVVQSIDVSTIYEVPIKMQEEKLDVTALRKLKLPIGETPDLNKWTDFLYRHKNPKHEVRIGLIGKYVELQDSYKSILEAFIHAGAENEVKVHVEYIHSEHIDSATIPERFSKLDGILVAPGFGERGIEGKVDAVRYARENDLPFLGICLGMQMAVIEYARNVLGIKDANSTEMSPDTQNPVISLMENQKSITNMGGTMRLGAWTCDLAEDSIVGNVYGEKTIAERHRHRYEYNNSYKEQFEKAGLRATGTNPETGLVEIVEIPTHPWFVGVQYHPEYKSTVANPHPLFVAFVKAASEY"
  },
  {
    "reference": "MCKWILTNGASAFSQLPCCCCFFLLFLVSSVSVTCHDNGQAMQSPAEAINSSSSSSSSSPASAGRHVRSYNHLQGDVRRRKLYSYNNLSSFLYFFLLGILEITSVEIGVVAVKSINSNYYLAMNKRGKVYGSKEFNSDCKLKERIEENGYNTYASLNWKHNGRQMFVALNGRGATKRGQKTRRKNTSAHFLPMLV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.3. Incorporate a signal peptide in the protein design.4. A protein that contributes to positive regulation of hair follicle cell proliferation, positive regulation of DNA repair, mesonephros development, tear secretion by functioning in nucleus, plasma membrane, cell surface, collagen-containing extracellular matrix, extracellular space with growth factor activity, type 2 fibroblast growth factor receptor binding, heparin binding, chemoattractant activity. The designed protein sequence is ",
    "response#1": "SCTECIRVDKDCSFCTDESFEEPRCDLRENLLRSGCGEASIVYTQGEMRTLKNSSINVSLQRTQVSPQAMYMRLRAGEEMSFNMDVFQPKESPVDLYILMDFSYSMSDDLDNLKSMGHNLADFLQALTSNYTIGFGKFVDKVSSPQTDMRPEKLREPWHNADSPFSFKNVIRLTSNINHFSQELSKERISGNLDAPEGGFDAILQTAVCKDKIGWRKDSTHLLVFSTESAFHYEADGTNVLAGILARNDEQCHLDSHGTYVYDTKQDYPSVPTLVRLLGQHNIIPIFAVTNHSYSYYEKLHKYFPISEIGELQEDSSNIVELLRVAFERIRSKMDIRADFTPRALKTEFTSPEFEKTESGSFNITRGKVSKFHMHVKALEYVGGQHVCSLPEKDRQGVIHVKPTSLSDSLTVSTAVICDVY",
    "response#2": "GNFELEILEISNTNSHLLNGYCCGMPAELRATKTIGCSPCTTAFRLCLKEYQTTEQGASISTGCSFGNATTKILGGSSFVLSDPGVGAIVLPFTFRWTKSFTLILQALDMYNTSYPDAERLIEETSYSGVILPSPEWKTLDHIGRNARITYRVRCTCAEGLSGEQC",
    "response#3": "PVLTGTHPVNTTVDFGGTTSFQCKVRSDVKPVIQWLKRVEYGSEGRHNSTIDVGGQKFVVLPTGDVWSRPDGSYLNKLLISRARQDDAGMYICLGANTMGYSFRSAFLIVVANRGLRVPFGREVWLDPLRDLVLQVQPGDRCTVTVLDNDALAQRPGHLSPKRFACDYGPGEVRYSHLGARSPSRDRVRLQLRYDAPGGAIVLPLALEVEVVFTQLEIVTRNLPLVVEELLGTSNALDDRSLEFAYQPETEECRVGILSGLSALPRYGELLHYPQVQGGAGDRGTSKTLLMDCKAFQELGVRYRHTAPSRSPNRDWLPMVI"
  },
  {
    "reference": "MAVLQWKLSRVIFVLLIIGISSFLIYFQFGNSFSASPALERLQDVFSKPSVQEYDYLVPLDEDMQGLSTTYHQATINEDDPQEPKSHEIVILLASDGRSSGNMAPETFNQCIENRINYAKHHNYGFEYVNVSQMNIPPVWAKMPAIIQTMNKHPHAKWIWWLDQDALILNTELSIQEHILSPDVLVEKLMKNEPMISPFSADLERLTPSSYTVDSARSLGLLISQDLNGLNAGSFFVRRSPMMALFLDLWGDKSFRENKVADHEQALLGYFVRYHPEIAAIIGILPQTLINSYPVGQPEMGWKEGHLVIHFAGCWVENRCQVLWDEYRERVH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have alpha-1,2-galactosyltransferase activity within Golgi membrane to regulate protein N-linked glycosylation, N-glycan processing.2. The designed protein should have at least Helical; Signal-anchor for type II membrane protein transmembrane domains to ensure proper localization.3. For general function, the protein need meet that Involved in the galactosylation of the N-linked core oligosaccharide Man(9)GlcNAc(2).4. The Cytoplasmic, Lumenal topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells. The designed protein sequence is ",
    "response#1": "GCTVAQNGGFFRMETGECLGNVVSGGRRVSSAGGLQNAQFGIRRDGTLVTGYLSEEEVLDTENPFVQLLSGVVWLIRNGSIYINESQAAECEETQETGSFNRFVNVISARTAVGHDRKGQLVLLHVDGQTEQRGINLWEMAEFLLKQDVVNAINLDGGGSATFVLNGTLASYPSDHCQDNMWRCPRRVSTVVCG",
    "response#2": "GCTVAQNGGFFRMETGECLGNVVSGGRRVSSAGGLQNAQFGIRRDGTLVTGYLSEEEVLDTENPFVQLLSGVVWLIRNGSIYINESQAAECEETQETGSFNRFVNVISARTAVGHDRKGQLVLLHVDGQTEQRGINLWEMAEFLLKQDVVNAINLDGGGSATFVLNGTLASYPSDHCQDNMWRCPRRVSTVVCR",
    "response#3": "GCTVAQNGGFFRMETGECLGNVVSGGRRVSSAGGLQNAQFGIRRDGTLVTGYLSEEEVLDTENPFVQLLSGVVWLIRNGSIYINESQAAECEETQETGSFNRFVNVISARTAVGHDRKGQLVLLHVDGQTEQRGINLWEMAEFLLKQDVVNAINLDGGGSATFVLNGTLASYPSDHCQDNMWRCPRRVSTVVCT"
  },
  {
    "reference": "MDSSTVLFIGAVAVAFIFLKWMVSPIPPQNEFTIDHLSQDQSTGSTTSSNAHQNRDTHSISTQSHRNSRRAVSESMIEVVQSIAPMLTVEQIRYDLETTGNVEATVNRFMELGDLPFPPGYVRPQQPPTPSEEPKKQEVIGKRSVNLLEKYNIDAKNPKSDHNSLLHQRRQEMILGARKRLAAQLSNEL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be involved in ubiquitin-dependent ERAD pathway by localizing to membrane, endoplasmic reticulum membrane and exhibiting ubiquitin-protein transferase activator activity, ubiquitin binding.2. The designed protein have CUE domains with specific sequence motifs that are crucial for its activity.3. The designed protein should have at least Helical transmembrane domains to ensure proper localization.4. The Lumenal, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.5. For general function, the protein need meet that Recruits the soluble ubiquitin-conjugating enzyme UBC7 to the cytoplasmic face of the endoplasmic reticulum membrane where it functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system; Component of the endoplasmic reticulum-associated protein degradation (ERAD) pathway The designed protein sequence is ",
    "response#1": "MILTVKPLQGKECNVQVTENEKVSTVKELVSERLNIPASQQRLLYKGKALADEHRLSDYSIGPEAKLNLVVRPAGGTAQKRLLKELQQLIKDSPPGIVAGPKSENNIFIWDCLIQGPPDTPYADGVFNAKLEFPKDYPLSPPKLTFTPSILHPNIYPNGEVCISILHSPGDDPNMYELAEERWSPVQSVEKILLSVMSMLSEPNIESGANIDACILWRDNRPEFERQVKLSILKSLG",
    "response#2": "VHVKSPSNKYDVEIAADASVSELKDKVLVFVPTANKEQVCIIYTGKILKDEETLTQHKIADGHTVHLVIRR",
    "response#3": "MILTVKPLQGKECNVQVTENEKVSTVKELVSERLNIPASQQRLLYKGKALADEHRLSDYSIGPEAKLNLVVP"
  },
  {
    "reference": "MTELRHRGGAADPDLQQSEDKGSEAEDRMEKDGGVSDSDSKPDSGGPEVPAPSDDTPEVLNKALSGLSSRWKNWWVRGILTLAMISFFFFIIYLGPMVLMMIVLCVQIKCFHEIITIGYSVYHSYDLPWFRTLSWYFLLCVNYFFYGETVTDYFFTLVQREEPLRILSKYHRFISFALYLSGFCMFVLSLVKKHYRLQFYMFGWTHVTLLIVVTQSHLIIHNLFEGMIWFIVPISCVICNDIMAYMFGFFFGRTPLIKLSPKKTWEGFIGGFFATIVFGILLSYVMAGYRYFVCPVEFNNDSNSFTVYCEPSELFQLQDYTLPSVLQSLTGWTTVKLYPFQIHSIALSSFASIMGPFGGFFASGFKRAFKIKDFANTIPGHGGIMDRFDCQYLMATFVNVYIASFIRGPNPTKVIQQLLALRPDQQLYIYNSLKAHLTERARSKSVIPGDGGAGSGRSDVKRSMEKPLKKGWLKKQRSIVKNWKPCYFVLRGNILTYHKDDRESAVQGTIPLWASQVNEVPINTDDKFIFEIIPRSNGDRERDGCVLMASSQSEMEDWVRLIRRAIGAQGSGVFGKGLADIMAYEKKLGPRSVPMLVEKCAEFIREHGLDEEGIFRLPGQENQVKHFREAFDAGERPSFPSDTDVHTVASLLKLYLRELPEPVVPWTQYQDFLDSSIMLDATTAAGKEKLEKQIGLLPKANYNLLSYICRFLFEVQQHSKVNKMSVENLATVMGVNLFKPQVEDAFSMMKGTPMIQKVMTVMIRHHEL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be designed to localize specifically to membrane in order to perform its role in signal transduction more effectively.2. The protein have enhanced a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-   diacyl-sn-glycerol + diphosphate.3. The designed protein should interact with enzymes and substrates within the Phospholipid metabolism, CDP- diacylglycerol from sn-glycerol 3-phosphate: step 3/3 pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "YQGLNINNAATVRRLAQDGHEIVYVPCHRSHMDYLLLSYVLYHEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNRLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPAKTGMLAMTIQAMLRGLNRPVTLVPVYIGYEHVMEVATYAKELRGKRKEKENAGLVLRTLRKLRNFGLGYVNFGEPIPLNQYLY",
    "response#2": "IVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFTRGYSVEYFVEGGRSRTGRLLEPKTGTLSMTIQAMLRGGSRPITLVPIYIGYG",
    "response#3": "IVYVPCHRSHMDYLLLSYVLYHEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNRLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPAKTGMLAMTIQAMLRGLNRPVTLVPVYIGYH"
  },
  {
    "reference": "MAYDLSEFMGDIVALVDKRWAGIHDIEHLANAFSLPTPEIKVRFYQDLKRMFRLFPLGVFSDEEQRQNLLQMCQNAIDMAIESEEEELSELD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Involved in the control of Yop release. The designed protein sequence is ",
    "response#1": "DFGFVESYNAVVRVAVDHEKKYLYIYWEYYKNGLTDDKTAEELKEFAETKELIKADSAEPKTIRYFQQHGFNMVGARKYQGSRLQYTKKIKRFRKIICSDRCENTIYELKPLAYATDKLGNIIEDEFTIDPHTLSAIWYALDR",
    "response#2": "TPKNILMIGPTGVGKTEIARRLAKLANVPFIKVEATKFTEVGYVGKEVESIIRDLTDVAVKLTHQQAMEKVKFRAEELAEERVLDALLPPPRDAWGQAEQKEENSSTRQVFRKKLREGQLNDKEIEINVAVPQMGVEIMAPPGMEEMTNQLQGLFQNLAGDTKKKRKMKIKDALKALVEEEAAKLVNQEELKEQAIYNVENNGIVFIDEIDKICKRGEVSGPDVSREGVQRDLLPLIEGSTVSTKHGMVRTDHILFIASGAFQVAKPSDLIPELQGRLPIRVEX",
    "response#3": "EIMAPPGMEEMTNQLQGLFQNLAGDTKKKRKMKIKDALKALVEEEAAKLVNQEELKEQAIYNVENNGIVFIDEIDKICKRGEVSGPDVSREGVQRDLLPLIEGSTVSTKHGMVRTDHILFIASGAFQVAKPSDLIPELQGRLPIL"
  },
  {
    "reference": "MKETIISDIHCIITKPDRHNLITVVVETNEGVTGFGCATFQQRPLAVKTMVDEYLKPILIGKNANNIEDLWQMMMVNAYWRNGPVINNAISGVDMALWDIKAKLAGMPLHQLFGGKSRDAIPVYTHATSDTMEGIYDLVEGFLEKGYKHIRCQLGFYGGVPTDLHTTQNPTEGSYYDQDQYMDNTLTMFKSLREKYGNQFHILHDVHERLFPNQAIQFAKEVEQYKPYFIEDILPPNQTEWLDNIRSQSSVSLGLGELFNNPEEWKSLIANRRIDFIRCHVSQIGGITPALKLGHLCQNFGVRIAWHCPPDMTPIGAAVNTHLNVHLHNAAIQEHVEYNGNTHKVFPNAAEPINGYLYASEIAGIGVEIDREAAAEFPVMYRPHEWTQSRLPDGAIHTP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that May have evolved a divergent function.; Has no detectable activity with D-mannonate and with a panel of 70 other acid sugars (in vitro), in spite of the conservation of the residues that are expected to be important for catalytic activity and cofactor binding2. The protein should be able to bind Mg(2+), D-arabinonate ligand in a variety of conditions3. The protein must be able to magnesium ion binding.4. The target protein must exhibit strand-strand-strand-strand-helix-helix-helix-helix-helix-strand-helix-strand-helix-helix-strand as its primary conformation. The designed protein sequence is ",
    "response#1": "MIVGVGIDVLEVERVPEKFAERILGESEKRLFLTRKRRREFIAGRFALKEAFFKALGTGLNGHSFTDVEFLESNGKPVLCVHKDFGFFNYAHVSLSHDRFAVALVVLEK",
    "response#2": "MIVGVGIDVLEVERVPEKFAERILGESEKRLFLTRKRRREFIAGRFALKEAFFKALGTGLNGHSFTDVEFLESNGKPVLCVHKDFGFFNYAHVSLSHDRFAVALVVLESH",
    "response#3": "MIVGVGIDVLEVERVPEKFAERILGESEKRLFLTRKRRREFIAGRFALKEAFFKALGTGLNGHSFTDVEFLESNGKPVLCVHKDFGFFNYAHVSLSHDRFAVALVVLEK"
  },
  {
    "reference": "MEKLTILLLVAAVLMSTQAMFQGDGEKSRKAEINFSETRKLARNKQKRCGGYSTYCEVDSECCSDNCVRSYCTLFG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Ensure the presence of a signal peptide within the protein.2. The designed protein should have a specific function within the extracellular region.3. For general function, the protein need meet that Potent neurotoxin. The designed protein sequence is ",
    "response#1": "GLLH",
    "response#2": "SMSPQTETKASVG",
    "response#3": "SVPPF"
  },
  {
    "reference": "MPYKLQESFLNTARKKRVKVSVYLVNGVRLQGRIRSFDLFTILLEDGKQQTLVYKHAITTIVPHERLEIEFEEAGVPGQG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have RNA binding within cytosol to regulate regulation of DNA-templated transcription, regulation of RNA stability.2. For general function, the protein need meet that Also binds with high specificity to tRNAs.; RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations3. The designed protein should contain one or more Sm domains that are essential for its function. The designed protein sequence is ",
    "response#1": "ETPDPFLNALRKERIPVSIYLVNGIKLQGQVESFDQFVILLKNTVSQMVYKHAISTVVPSRARR",
    "response#2": "MTKDIFLNSARKNKMPVTIYLSTGFQINGTVKGFDSFTVILDSEGKQMLIYKHAITTVTPEKPE",
    "response#3": "MDIFLNQVRKEHIAVTIYLTNGFQLKGLVKGFDNFTVVLDTDGKQQLVYKHAISTISPMKSI"
  },
  {
    "reference": "MITVNIPGSKSISNRALLLASLAGPRKIAPKNLLQSDDTKYMQQALKALKKGKKSLYCGNAGTAMRFLAAYVATLEQTTTLNGNARMRERPIKDLVDALRQLGAEVEYLKHKGYPPLRIRGPLLGGTCTLQGTISSQYLSGLLMAAPLAKKNVTIHIRGGLVSKPYVDMTISMMETYGVKVARDGYKSFRISAGQKYKARSYTIEPDASSASYFYGIGMLTQNKMHIPNLDLKNSLQADVRFVKAATALQAKKMSRHIDCESFPDAAMTLVVLAAFTKGKSEFTGLGNLRVKECDRLSALATELKRIGCRVTEKKEGIIVYGNPEKLHGAPIRTYDDHRMAMCFGMASVLIPDITIENPQCVKKTFPTFFIELKKVKKQLNEQNIILSGMRGCGKSTLAKALAKKMKREYIDLDTVIEKRAGKTIAEIVAAKGWNYFRTLEAQAVTKISRKKNIIIAVGGGTVLFQKNVKQLKQHGKIIYLNCSVSSIQKALAKNKHRPSVTKESVLEEAKALYAKRHPIYAKNADVIISGVEHTTNKEKDLTKKVAKLSAHCAYFGIHETV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.2. The Proton donor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The protein can be expressed in cytoplasm and perform ATP binding without triggering undesirable cellular responses.4. The 3-phosphoshikimate, phosphoenolpyruvate, Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.5. Mg(2+) is required for the proper folding and stability of the protein.6. A protein that is capable of catalyzing reactions within the chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7, chorismate biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MIKGEVNALPPDKSIAHRAALIGAVADGTTEISNFSGGFDNQSTLGVLQACGIAVHQEKLKGPNGLISRNVVIHAQGFWSLSRPEKDLMCNNSGSTMRMFAGILAAQPFESRLAGDSSLMKRPMQRVAHPLRLMGADVALSSDNTAPVAIRGTTHLRPIDYELPVPSAQVKSLVALAALHAEGESRVIETLPSRNHTELMLGLKVEALSDGRSAVIIPGRKTVPAKPFHIPGDPSAACFIIALGLLSAGSEIVVRDVCLNPTRTGYMGVLLGAGAEISMENIRFAGGEKIGDVLVRHSPSMKPLSISDPAVVANSIDEIPMLAVLSACATGAFELYNAAELRAKESDRISAVVDNLERIGFVCDEYADGFAVTGRKDVTPTGKVAIDSYDDHRIAMSFAIADRVVPMELDISDAKVIGVSFPDFFEQYVRE",
    "response#2": "MIKGTFFNLPPDKSISHRTALIGALSNGITEITNYSAGLDNQTTLSVLQSLGVDIQQESFLTNDGYEQRKVVIKSSGLWSLSRSKRELMCNNSGSTIRMLSGILAAQPFRTTLVGDHSLMKRPMRRVAEPLTQMGASIKLSETGAPPIEIEGKKPLSPIQFYQKIPSAQVKSLVIFAALHADGISEIIEPIQTRNHTELMLGLEPELQPDGSRKIVIEGQKNIEAKPFTVPADPSGACFLVALGLLSGKSDIRLKNVGLNHTRAGYLSLLREAGAKLPTENNRTIGGELLGDIIISNDYITKPLRINDAQIVSDIIDEIPMLAVLSAMATGEFELHHAAELRAKESDRIHALVMNLQRLGFVCEEYKDGFCVTGRTHNPTGTIEITTFYDHRIAMSFAIAGHFSNAEIQLDDNSSIAVSFPNFFND",
    "response#3": "MKPINKTLTIPGSKSYTNRALFLAAISSNPVKIINPLISDDTLAMIGCLKKLGIQISIKKNSFEVLNNIMSIKNQSYNLNANESGTTLRFILALSTIIPGVKTLYGEEGLNKRPIGELVAALQKLGANIKYLDKKGYPPVQVSSSKLRPETIKIKGSISSQYISALLMIAPFLGEVKIEIEGNQISKPYIDMTIKAMKQFGVNVINEKYKKYFIQANQKYAAKKYTIEADYSSASYFLAIAALTKSNITLRNINPESKQADKKFIKILEKMDNKIKFANNQLTIAGKGVKPVNIDMTDCPDQIQTLAVLAAFAQGITKISSIQSLRIKETDRVAALQKELKKMGIKTSANTNTLIIYGGNPQPAKIETYGDHRMAMSFAVAGAKIAGIEIVDPDVVSKTFPDFWKKLDQ"
  },
  {
    "reference": "MKTYRRRIREKIIQALYTIELTGADMDSASDWLITPQIAEDSGAVRFYKQVLQSIQDHKEEIDGYITKHTFNWDMSRIAIIDKNILRMALAELLYFEDIPPKVSINEAIEIAKKFNSTDKSSKFVNGILDATYNDLKSSGKIKKCGRGLINNSSRNTSRSEEKHSTEK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must possess RNA binding to support DNA-templated transcription termination, transcription antitermination under certain conditions. The designed protein sequence is ",
    "response#1": "MTGSVKWFNETKGFGFLTQDNGGNDVFVHFNSIQSEGFKTLAEGQRVSFIVEQGKKGPQASNVVG",
    "response#2": "MTGSVKWFNETKGFGFLTQDNGGNDVFVHFNSIQSEGFKTLAEGQRVSFIVEQGKKGPQASNVVG",
    "response#3": "MTGSVKWFNETKGFGFLTQDNGGNDVFVHFNSIQSEGFKTLAEGQRVSFIVEQGKKGPQASNVVS"
  },
  {
    "reference": "MAVSLSASARRRTAKLRRHVRVRKKVSGTPIRPRLVVTRSSRHIYAQVIDDVAGHTLASASTLEDGLRASDGDKSAKAREVGRLVAERARAAGIDAVVFDRGGRTYHGRIAALADAAREGGLNF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be optimized for ribosome-specific folding and stability, while maintaining structural constituent of ribosome, rRNA binding. The designed protein sequence is ",
    "response#1": "GRGNRICFGRFALQALEPAWITSGQIEAGRRTINRYARRGGKIWVRIFPDKPITMRPAETRMGSGKGSPEYWVSVIRPGRILYEMGGVSETVARAAARIAAYKMPIRTQFN",
    "response#2": "RMGSGKGSPEYL",
    "response#3": "MPSLSMRQMLEAGVHFGS"
  },
  {
    "reference": "MAVAISGSSLISSTLQHYNNRIYIRDYPIPCHSSNPICNSFNFKRRSFSPSSPKFNDHVVNPSSSYLSSKLSPIRTHSSFAACGCSWIQDNSMVHDYATTTNGTSKRCSALPTTNTVDVSSVSDLFEFICSGPLVNKIGITPQRVGQSIDKWLLYGSQLCRLFQLNELKLTIPQKARLYHYYIPVFIWCEDQIALHNSKFKDGDDVPPLVIGFSAPQGCGKTTLVFALDYLFKTTKKKSATISVDDFYLTAEGQAELRKKNPGNALLEYRGNAGSHDLKLSVETLEALSKLTKEGLKMKVPRYNKSAYSGRGDRADSSTWPEVEGPLSVILFEGWMLGFKPLPADVVKAVDPQLEVVNKNLEAYYDAWDKYIDAWVVIKIQDPSYVYRWRLQAEIAMRQDGQAGMSDEEVNDFVSRYLPAYKAYLPTLYAEGPSGSDPDRVLAIDIDEERNPILAN",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in chloroplast envelope, mitochondrion, cytoplasm, chloroplast stroma, chloroplast that is critical for photorespiration, phosphorylation.2. For general function, the protein need meet that [Isoform 3]: Cytoplasmic D-glycerate 3-kinase that constitutes a photorespiratory bypass that alleviates fluctuating light-induced photoinhibition.3. The designed protein should interact with enzymes and substrates within the 3-phospho-D-glycerate from glycine: step 4/4, Photosynthesis, photorespiration pathway to enhance or regulate its activity.4. The designed protein should have a binding site that is specific to the target ATP ligand. The designed protein sequence is ",
    "response#1": "MAFTGSPGTGKTTVAMRMGQILKAMGYSRSGHLIVATRDDLVGQYVGHTAPKTKEVIKRAFGGVLFIDEAYYLYNAANDRDYGVEAIEILLNVMEEQREDLVVIFAGYEDRMNKFYSYIPGISSRIGNHISDDAIPACRAYIEKRMQMPYFSNARTVRNLINRARMRSAIRIFNKAMDPNADGLVSRDELMTLITEDKPAEKSSDQYFKAAKIADAFERDIHYTVDEKQKSVLLSEQGYEDAEEILAVKDLYDPREQWASFVINAIKAKELFLRDVNYIIRGKEVLIVDEFTGRVMQGRRWSDGLHQAVDDAIPACRAYIEKRMQMPYFSNARTVRNLINRARMRSAIRIFNKAMDPNADGLVSRDELMTLITEDKSPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRIMQRLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHVTLVQQ",
    "response#2": "TSVNEDQIRNDMDKDIPSGNDSDNSNYSNVDHLIGVSSDRIELLIDPGTWDPMDEDMVSLDPIEFHSEEEPYKERVDSYQRKTGLTEAVQTGTGQLNGIPVAIGVMDFQFMGGSMGSVVGEKITRLIEYATNQNLPLILVCASGGARMQEGSLSLMQMAKISSALYDYQSNKKLFYVAILTSPTTGGVTASFGMLGDIIIAEPNAYIAFAGKRVIEQTLNKTVPEGSQVSEYLFHKGLFDLIVPRNLLKGVLGIKNSPSNKINRVNSFSLCREL",
    "response#3": "SFTGSPGTGKTTVARKMADILYRLGYIKKGHLITVTRDDLVGQYIGHTAPKTKQVLKNAMGGVLFIDEAYYLYRPDNERDYGAEAIEILLQVMENQRDDLVIIFAGYKDKMDRFYTSNPGLASRVANHVNH"
  },
  {
    "reference": "MTAVTECFRSLRSQGNCALIPFITAGDPDLSTTAQALRILDRAGADLIELGVPYSDPLADGPVIQSAATRALNRGVKLEDVLEIVKNAQGEVKAPIILFTYYNPIYHRGIDVFLDQIKAAGVSGLVVPDLPLEEAESLLQPAAAKGIEVILLVAPTSPPERIQAIALQSQGFIYLVSVTGVTGMRKQVATRVEELLDSIRSVTDKPVGVGFGISEPTQALQVKNWGADAVIVGSAMVKRLADNSPSDGLKSLEEFCRSLKQAIQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must be able to perform tryptophan synthase activity under specific conditions.2. The protein should be optimized for activity in L-tryptophan biosynthesis, Amino-acid biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "GRPSSLHKAERLTEHCQGAQIWLKREDLNHTGSHKINNALAQVLLAKRLGKKNVIAETGAGQHGVATATACAKFGLTCTVFMGAEDVRRQALNVFRMRILGAKVIAVTNGTKTLRDATSEAFRFWVTNLKTTYYVVGSAIGPHPYPTLVRTFQSVIGKETKEQFAAMNNGKLPDAVVACVGGGSNSTGMFSPFEHDTSVKLLGVEAGGDGVDTKFHSATLTAGRPGVFHGVKTYVLQDSDGQVHDTHSVSAGLDYPGVGPELAYWKSTGRAQFIAATDAQALLGFKLLSQLEGIIPALESSHAVYGACELAKTMKPDQHLVINISG",
    "response#2": "GRPSPIYHAKRWSEMLGGAQIYLKREDLNHTGAHKVNNCIGQAMLAKRMGKPRVIAETGAGQHGVATATVAARYGMECVVYMGSEDVKRQAANVYRMKLLGATVVPVESGSKTLKDALNEAMRDWVTNIHNTFYIIGTVAGPHPYPMLVRDFQKIIGEECLVQMPEMTGRQPDAVIACVGGGSNAMGIFYPYINVEGVRLIGVEAAGDGMETGRHSASLTAGVPGVLHGNRTYLLQDEDGQIIETHSVSAGLDYPGVGPEHAWLKDLGRAEYQPVTDSEALAAFHTLCRIEGIIPALESSHALAYAAKLAPTLAKDKILLVNY",
    "response#3": "TEHVRAIAAHAGGRGGAKVYFKREELNHTGAHKINNVLGQILLARRMGKTRIIAETGAGQHGVATATACARFGLECVIYMGAVDVERQKPNVFRMNLLGAKVVPVQSGARTLKDAMNEALRDWVTNVTDTFYCIGTAAGPHPYPAMVRDFQCVIGEETRTQMLEREGRLPDSLFACIGGGSNAIGLFHPFLDEPNVEIYGVEAAGFGLDKQHAASLAGGRPGVLHGNRTYLLMDADGQIEEGHSISAGLDYPGIGPEHAWLKETGRVTYLSARDDEALDAFQLCAKLEGIIPALEPAHALAKVVEVAPSKPWL"
  },
  {
    "reference": "MQTLTVELGDRSYPIHIGRGLLDDPTRFAPRIRGKRVMIVTNETVAPLYLDRLKRTLGDFRVEAVILPDGEEYKTMETLNQVYTALLEARFDRKATLVALGGGVIGDITGFAAASYQRGVDFIQVPTTLLSQVDSSVGGKTGVNHPLGKNMIGAFHQPRCVVIDTDTLDTLPDRELRAGIAEVIKYGLICDRPFFDWLEANMDRLLARDPEALAYAIHRSCHDKAQVVAEDEREGGRRAILNLGHTFGHAIETGMGYGVWLHGEGVATGMVMAARMSRRLGWLDAEDLHRTEALIARAGLPVEPPPEITAERFAELMSVDKKVLDGQLRLVLLRGIGEAVVTADFDPAALDATLNQVGSGN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should be able to enhance the efficiency of chorismate biosynthetic process, amino acid biosynthetic process.2. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.3. A protein should be able to manipulate the activity of enzymes in the chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7 pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "VQPKGPLQGSVHVPGSKSISNRVLLLAALGKGTCRISGLLHSDDTQVMMDVLQYLGCGFAWEDDGNVLVVHGTGGVFPKTMPTHWYLSNAGTAARFLTSVATFCGAEITLTGNHRMQERPIADLVDALNTNGCHIAYDKTSGCPPLRITPTGLPGGPMRMQGKVSSQYVSSVLLSAPYASSPLDLLLEEDAPTSLPYILMTTQLMADFGIRVQQTGANRFLVPRGVYTNPATYHVEVDASSATYPLALAAITGGRVTVPGLGSTSTQGDAAVHTVLQAMGCTTGQDAHSTWVQGPACGTLQAVNVDMMTMTDAFMTVAVVAAAANGKTTITGIANQRVKECNRIEVMVQELAKCGVLCGELPDGIWIQGLGGKAPIFPQTLAKIACHNDHRIAMSFAVLGAVR",
    "response#2": "GMAEIIKAGAIFSEPLFSLLETNVDAILSLQKDIVLDVVAQSIAVKTTVVNLDVSEQGIRAILNFGHSIGHGIEAIMQPELLHGECVAIGMVKEAEIARGMGLCSSATVGRLLRCIKAFGLPVRVPSRSPSHVVLTNMEVDKKL",
    "response#3": "GLAEVVKYGILDKEVYNAIKTMNSPREIVEREDIIKKSVAVKLRIVEEDLKENGKRRVLNLGHTVGHAIEKISDYKIKHGFAVSMGLVVEAKIGEILYGFDSGKVVEILTKFGLPVKIPFDPYTTLQAMKIDKKL"
  },
  {
    "reference": "MDYLPIFCQLRDKPCLLVGGGEVAERKAKLLLDAGAILTVNAISFSEKFMRWQQEKRATLVQGAFDSGLLTGVWLVIAATDDRETNKQVYDYASQQQIFCNTVDDVQSASFIMPSIIDRSPLMVAVSSGGTAPVLARILREKLESILPQHLGKLASLAGELRQRVQNRFRKADTRRRFWEKLFIHDRLAQSLANNDAASAQQLTERLFSEPLDDRGEVVLVGAGPGDAGLLTLKGLQKLQQADIVVYDRLVSAEVLNLIRRDAERIFVGKQSGHHCIPQEQINQLLQEKALAGKRVVRLKGGDPFIFGRGGEELEHLQQAGISFSVVPGITAASGCAAYSGIPLTHRNYSQGVRLITGHVQHDTELDWASLAAEKQTLVFYMGLQQADYIQRKLIEQKLPETVPVALIENGTSTAQRVFTGQLSQLGELAKQAVSPSLIIVGNVVGLREKLNWFTQRSE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The Proton donor active site of the designed protein must be optimized for substrate binding and specificity.2. We require a protein that can specifically bind to the metabolites produced in the siroheme biosynthesis, Porphyrin-containing compound metabolism, sirohydrochlorin from precorrin-2: step 1/1 pathway.3. The protein should have a high affinity for S-adenosyl-L-methionine, NAD(+).4. A protein with sirohydrochlorin ferrochelatase activity, NAD binding, uroporphyrin-III C-methyltransferase activity, precorrin-2 dehydrogenase activity functions is required to enable cobalamin biosynthetic process, siroheme biosynthetic process.5. The presence of Tetrapyrrole methylase, Siroheme synthase central domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "VGAGPGDPGLLTLRGLQLMQQADVILYDYLVSDDVLELCRRDAEMICVGKRAGFHSVPQEETNALLVKFASRGKRVVRLKGGDPFMFGRGGEELQVVAEAGIPFQVVPGITSAAGATAYAGIPLTHRDHAQSVMFITGHLKKEKDSLDWSTLARGNQTLVIFMGLMKSDHIRDQLIKHGRAAHTPVAIIENGTRSEQRVLKGTVHDLATLAEHASSPALIVVGEVLAGGATAEA",
    "response#2": "VGAGPGDAGLMTIKGLQQCQQADVVVYDRLVSDEVMALVRRDAQRIYVGKQAGFHCVAQDEINQILINEATAGKRVVRLKGGDPFIFGRGSEELEALIDSQIPFSVVPGITAASGCTTYAGIPLTHRDYAQSVRFITGHGKGLNDAQWQCIAQKNQTLVFYMGLAKAAFIQEKLLSQNMSSTTPVAIIEKGTLACQKMIAGQLHQLSEMAKTMESPSLIIVGDVGADGWACGYTGEGRMVGAGPGDPDLLTLKALRLMYAADVVLYDRLVSPEIMKRVRPDADKVFVGKEAKHHPVPQEQINDMLVRLAREGKKVLRLKGGDPFIFGRGGEEIDQLAAAGLPFQVVPGVTAASGCAAYAGIPLTHRDFSQSVRFVTGHMKNDEPDLDWPVLAKEDQTLVIYMGLITLPVICEQLQAYGMNSAMPVAIVEQGTLPQQRVVTGTLQDIAGKVEAAGVKAPAIIIVGQVVGAGPGNPDLLTFRALRLMQQADVVLYDKLVAPELLELVRRDAERIYVGKARADHTLPQDDINSLMVSLAKAGRRVLRLKGGDPFTFGRGGEEIDTLAAEGIPFEVVPGITSASGAAAYAGIPLTHRDYAQSVVFVTGHMKDGSFNLDWPALTRPQQTVVVYMGLSHIQGLCDGFILHGKAADTPAAIVEQATTVRQRTLTGTLADLPALAQAHGIASPALIIVGEVVASS",
    "response#3": "VALVGAGPGDPGLLTLRGLQLMQQADVILYDYLVSDDVLELCRRDAEMICVGKRAGFHSVPQEETNALLVKFASRGKRVVRLKGGDPFMFGRGGEELQVVAEAGIPFQVVPGITSAAGATAYAGIPLTHRDHAQSVMFITGHLKKEKDSLDWSTLARGNQTLVIFMGLMKSDHIRDQLIKHGRAAHTPVAIIENGTRSEQRVLKGTVHDVVLVGAGPGDPGLLTLKALRALNEADVILYDRLVSADILSLARRDAEREDVGKRPGEDHAATQARIHALMVEHARRGRRVVRLKGGDAFIFGRGGEELQHLRAHGVPYEVVPGVTAALACAAHAGIPLTHRDHAQSLHLITAHCRDDDDAVDWGALAGSRQTLAFYMGVSQLDALARRLQEHGRAADTPFALVENGSRPEQRVLTGRLAELRALGASVGSVVLVGAGPGDPGLLTLKALRALNEADVILYDRLVSADILSLARRDAEREDVGKRPGEDHAATQARIHALMVEHARRGRRVVRLKGGDAFIFGRGGEELQHLRAHGVPYEVVPGVTAALACAAHAGIPLTHRDHAQSLHLITAHCRDDDDAVDWGALAGSRQTLAFYMGVSQLDALARRLQEHGRAADTPFALVENGSRPEQRVLTGRLAELPALARAHDIRAPALLIVGRVAALRRRLGLE"
  },
  {
    "reference": "MAGTVLGVGAGVFVLALLWVSVLLLCALLFRASGAARFSVIFVFLGALIVTAILLLFPRASDAPAPEAETKIVDAFFIGRYVLLAFLTAVFLGSLFLVLIHHILEPIYAKPLRSY",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be expressed specifically in the cilium, membrane2. For general function, the protein need meet that May be involved in ciliary biogenesis or function. The designed protein sequence is ",
    "response#1": "ARAWQHPFLNVFRHFRVDEWKRSAKQGDVAVVTDKTLKGAVYRIRGSVSAANYIQLPKSSTQSLGLTGRYLYVLFRPLPSKHFVIHLDVSSKDNQVIRVSFSNLFKEFKSTATWLQFPLVLEARTPQRDLVGLAPSGARWTCLQLDLQDVLLVYLNRCYGHLKSIRLCASLLVRNLYTSDLCFG",
    "response#2": "ARAWQHPFLNVFRHFRVDEWKRSAKQGDVAVVTDKTLKGAVYRIRGSVSAANYIQLPKSSTQSLGLTGRYLYVLFRPLPSKHFVIHLDVSSKDNQVIRVSFSNLFKEFKSTATWLQFPLVLEARTPQRDLVGLAPSGARWTCLQLDLQDVLLVYLNRCYGHLKSIRLCASLLVRNLYTSDLCFK",
    "response#3": "DLNVDPSAPEPTGKVTFCINDRPQRVVMWLNQNFLLPEGIDSPDVTFSALRGGGLLRISLQTSGEITLRTDDIDLAGDLVQSLASFL"
  },
  {
    "reference": "MSASERQHTLAKPASITGISLHTGEQVTLTLQPAPEDFGFKFRRIDLEDKPFIPALVEKVQKVERATTIAEGGVNVHTVEHVISALTGMGVDNAIIEMDANEPPIVDGSSQPFVELIKKAGIVAQNAPRKVFEIREPIYQETRDGTILTIVPDKKFRISCTNVGPGGRFTQFISLEINPEVYEKEIAPARTFVYYEDIAPLMEKGLIKGGTLEAAVVVRGETLLSKQPLRFDNEFVRHKILDIIGDLMLCGRRITGHVIAVRPGHGPNTELARSIDAQYKQMRSMVPPPLAIPTGEAVLDVTQVMKLLPHRYPFLLVDRIIGLEGETKCTGVKNVTINEPFFQGHFPGHPVMPGVLQLEAMAQVASIVLMKMPEHQGKIGYFMSANNVKWRKPVFPGDTLIIETEMLKVKRSIAQATARITVNGVVVSEADLMFSVVDR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be optimized for (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity.2. The protein design should take into account the physiological conditions of the lipid IV(A) biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MW",
    "response#2": "MR",
    "response#3": "GVAE"
  },
  {
    "reference": "MPVLIATVGVVAVAALVIAIFVVIKFGRAPRVDAGNVRQPSGMSGPDAAAAEAEIREARSELERREQRLAEREARLDAEHERLAARERQLAELEEKLGRLQAELAQVAEERRLLLERTAGLTAEAAKAELVALIENQAKRDAALTVREIERAATEEAERRAREIVTTAIQRVASEQTAESVVSVVHLPGDEMKGRIIGREGRNIRAFESITGVNLIIDDTPEAVLLSCFDPVRREIARVALERLVDDGRIHPLRIEEVYEASRLEVERLCQRAAEDALLAVGITGMHPELVALLGRLRYRTSYGQNVLKHLVETAHLAGVMAAELHIDPQLVKRGALLHDIGKALSHEVEGSHALIGAELARRYGEHEDVVHAIEAHHNEVEPRTIEAVLTQAADAISGGRPGARRESLESYVQRLERLEEIASAYEGVEKVFAMQAGRELRVMVLPDVVDDIQAQVIARDIAKQIETELTYPGQIRVTVVRETRASEFAH",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have endoribonuclease activity, RNA binding and be active in plasma membrane. The designed protein sequence is ",
    "response#1": "SRGETLRTTAEALLAAVPYHVGEREVATLEAHFPQAEKNVVALEAHFPQAEKNVVALEGGCGPGNALSLMIQSEQLTELFAAFGVKGTSAEAVANQVAHEARRYG",
    "response#2": "GEGGGQILRSALSLSMITGQPFEMSGIRAGRAKPGLLRQHLTAVRAATEICGAQVNGDELGSQQLRFTPGPIRGGEYRFAIGSAGSCMLVLQTVLPALWFADGSSRVEVHGGTHNQAAPSADFICRVWEPLLARMGISQRTTLIKHGFYPAGGGAAATVVEPATSLRGLTLISRGETLRTTAEALLAAVPYHVGEREVATLEAHFPQAEKNVVALEAHFPQAEKNVVALEGGCGPGNALSLMIQSEQLTELFAAFGVKGTSAEAVANQVAHEARRYLASPAAVGEHLADQLILPLALAGEGAFTVARASAHLLTNIVVVERFLPVRFH",
    "response#3": "SRGETLRTTAEALLAAVPYHVGEREVATLEAHFPQAEKNVVALEAHFPQAEKNVVALEGGCGPGNALSLMIQSEQLTELFAAFGVKGTSAEAVANQVAHEARRYC"
  },
  {
    "reference": "MKYIFVTGGVVSSLGKGLAAASLGTLLEHRGLKVVLQKLDPYLNVDPGTMNPFQHGEVYVLADGAETDLDLGHYERFTNCVLTRENNVTSGQVYETVLANERRGDYLGKTVQVIPHITDEIKRRIRALGENSGADILITEIGGTTGDIEGLPFLEAIRQFQLEAGHGNVLVMHVTLVPYIKAAGELKTKPTQQSVAKLREIGLQPQVLICRTENPLDLDVRQKLSLYCNVPVEAVIEAGDVEHTIYESPLNLQAEGLDDYVCRILNLNTPEPDMSDWKKFVQRVVNPKKSVRIAVVGKYIELQDAYKSIYESLTHAAAANDCAADLVLVDAEDLENEGAEKYLQGVAGVLVPGGFGDRGTEGKIAAARFARESRTPFLGLCLGMQIATIEFARNVCGLDHANSTEFDPQTPHPVIHILEEQRDVTTKGASMRLGTCPTIFSKNSLAHQVYGRPEANERHRHRYEFNMAYREQMEKKGFVISGTSPDGTLVELIEIKNHPWFLACQFHPEFQSKPNGSHPLFKGFIAACLAHQD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein with ATP binding functions is required to enable 'de novo' CTP biosynthetic process.2. The presence of Glutamine amidotransferase domains is critical for the interaction of the designed protein with its intended targets.3. The designed protein must have a Nucleophile, active site that is structurally stable and resistant to denaturation.4. The protein should be able to bind L-glutamine ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "IKVGMVGKYVRFTDTYLSIYEALRHAAAKNGVGLEVKWVSAEQLEGGTLELLEGCQGVVIPGGFGARGAEGKILAIKWARERGVPLLGLCFGFQLAVVEFARNVVGLEGANSVEVDPSTPHPVVDLLPEQREVKELGGTMRLGAQEVVIEKGTRAFQIYGAERIFERHRHRYEVNPEYLPQLTGHGLVFSGKSPDGRRMEILEIPNHPFFLATQFHPEFKSRPLRPAPVFDAFIAA",
    "response#2": "LTDSYVSMNEALRHGGAECGARISIDYIEAEEFEENPDKINTLKEYDGIFVPYGFGPRGTEGKIKAINFARENDIPFLGICYGFQLAIVEFARNVCNLKGANSTEIEQEPAHPVIDLMPEQREITYKGATMRLGAHKVIIREGTLAHRLYGSTEIYERHRHRWEVNPKYWEILQEHGAVFSGMSPDHRRIEIFELPEKYFFFASQFHGEFKSRPGKPEPEYYGFR",
    "response#3": "LTDSYVSMNEALRHGGAECGARISIDYIEAEEFEENPDKINTLKEYDGIFVPYGFGPRGTEGKIKAINFARENDIPFLGICYGFQLAIVEFARNVCNLKGANSTEIEQEPAHPVIDLMPEQREITYKGATMRLGAHKVIIREGTLAHRLYGSTEIYERHRHRWEVNPKYWEILQEHGAVFSGMSPDHRRIEIFELPEKYFFFASQFHGEFKSRPGKPEPEYYGFR"
  },
  {
    "reference": "MLDIAMPKIEVVTAAENYGRFKIEPLDPGYGHTLGNALRRVLLSSIPGAAITKIKIEGVFHEFSTIPGVKEDVTEIVLNIKGIRLRSYAERPVKISLSKRGAGVVRAADIDAPSNVEIVNPNHYICTIDRDDAAIDMEMTVERGRGYLPADQRDALPIGEIPIDAIFTPVPKVNYVVEHIRVGQATDIDSLLIEIWTDGTIKPGDALSHAAQVLVQYSQTIADFNRLSTEAEPTTAPNGLAIPADIYDTPIEELDLSTRTYNCLKRADITKVGQVLEMDEKALLSVRNLGQKSMEEIRDKLIERGYIPRIGQTSHAARAEIEG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein have enhanced a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +   RNA(n+1).2. The designed protein must be able to perform DNA binding, DNA-directed 5'-3' RNA polymerase activity under specific conditions. The designed protein sequence is ",
    "response#1": "VKIEGIKHEFTVLDDVIEDVVTILNNLKRVRFFYNPSVFSQNSIHVASFLGQKAGQIYARDIESHSGLKIVNPDLYIADVSKVGALKFELFITNGKGFVDFETNKKYVNEVISLLESQIEGSVLAVDSDD",
    "response#2": "VKIEGIKHEFTVLDDVIEDVVTILNNLKRVRFFYNPSVFSQNSIHVASFLGQKAGQIYARDIESHSGLKIVNPDLYIADVSKVGALKFELFITNGKGFVDFETNKKYVNEVISLLESQIEGSVLAVDSDF",
    "response#3": "LIEVQKYSYDQFLQVDEPTGGREEQGLQAVFKSVFPISDFSESSTLEFVNYEFETPKYDVEECQQRGMTFAAPLKVTLRLIVFEVDEDTGAKSVKDIKEQDVYMGDMPLMTENGTFVINGTERVIVSQMHRSPGVFFDHDKGKTHSSGKLLFAARVIPYRGSWLDFEFDAKDIVFVRIDRRRKLPVTTLLYALGLDSEEILSTFYNSVTFTKVKQGWRVPFNADRYRGAKPERDLIDAKTGKVVVEAGRKVTPRLAKKLAEEGLKELLVQDEDIHTRYLAQEIVNMETGEIFAEAGDEITPELLEALVEAGHTDIITLDIDHVNTGAFIRNTLAVDKCTNREQALIDIYRVMRPGEPPTADTAEALFKSLFFDAERYDLSAVGRVKMNMRLDLDVSDTVRVLRKEDILAVIKTLVGLRDGKGEIDDIDNLGNRRVRSVGELMENQYRVGLLRMERAIKERMSSVDIDTVMPHDLINAKGAFISQTLRTDDTTDEYAAKVAIYRMMRPGEPPTEDAVNALFVNLFFSDERYDLSAVGRMKFNRRIGRDELTGTGTLSTEDIVAVIKILVELRNGRGEIDDIDHLGNRRVSGAFISQTLRTDDTTDEYAAKVAIYRMMRPGEPPTEDAVNALFVNLFFSDERYDLSAVGRMKFNRRIGRDELTGTGTLSTEDIVAVIKILVELRNGRGEIDDIDHLGNRRVSA"
  },
  {
    "reference": "MPLLTIEQAIEDIRFGKMVILVDDEDRENEGDLTIAAEKVTPEIINFMAKYGRGLICLSMTPDKLDQLNLPLMVGDNTSRFQTGFTISIEASKGVTTGISAHDRAHTVLTAIADKAKPEDLARPGHIFPLRARKGGVIVRAGQTEGSVDLARLAGLKPAGVICEIMNDDGSMSRMPQLEEFSEKHGIGICTIADLIEYRMRKESFVYRAAETIIPTIHGGQFNAIVFENHIDDLLNIALVKGKIDPEKPTLVRVHSECLTGDIFGSMRCDCGDQLHKALSKINDEGNGVLLYIRQEGRGIGLVNKIKAYALQDQGMDTVEANEKLGFPGDLRNYGIGAQILVDLGVKKMRLMTNNPKKMIGLEGYGLSVVEQVPIEIPPNDYNKKYLACKKLKMGHLLKSVECAP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a binding site that is specific to the target Zn(2+), D-ribulose 5-phosphate, Mg(2+), GTP ligand.2. The protein with the reaction GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate, the Proton acceptor; for GTP cyclohydrolase activity, Nucleophile; for GTP cyclohydrolase activity must be optimized to ensure efficient catalysis.3. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.4. The designed protein should have zinc ion binding, magnesium ion binding, manganese ion binding, GTP binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity to facilitate riboflavin biosynthetic process.5. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..6. Ideal protein should have a binding affinity for key enzymes in the Cofactor biosynthesis, riboflavin biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "RMVSTVLPTRNGRFDVHLYRSAVDTKEHIALVKGTIDAGAPVLVRVHSECLTGDIFGSQRCDCNEQLHTSMEMIEREGNGIVLYMRQEGRGIGLLNKLRAYKLQDEGKDTVEANEALGFHADVRDYSLAAQILLDLGVRRVRLLTNNPKKIVGLKGFGIEVTERIPKINDQEVTVHMPTRWGDFVCHAYTSPYGDNPGAVHIALVKGDISGDEPVLTRVHSECFTGDLLGSLRCDCGPQLHTAMSMIEKEGRGVLLYLRQEGRGIGLLAKLKAYDLQDKGLDTVEANLALGFAPDQRDYGIGAQILADLGLKKLRLMTNNPVKITGLSGYGLEITERVGVDLPTDFGDFELIAFRQTNTQELHLALIKGKWDKDEPILVRVHSSCMTGDIFGSCRCDCGAQLHGAMQMVQKEGKGVILYMNQEGRGIGLINKLKAYKLQEEGMDTVQANLALGLPSDSRDYGVGAQILRDLEVSKLRLISNNPQKRVGLLGYGLQIVEQVGVDMPTAWGDFELIAYEQTNTQEIHMALIKGKWEKDEKVLVRVHSSCVTGDIFGSCRCDCGPQLHRAMEMVQEAGKGVVLYMNQEGRGIGLVNKLKAYKLQEEGMDTVQANLALGFPMDKRDYGVGAQILRDLGISKIRLISNNPTKRAGLLGYGLEIVDTVERVAEARIPTIFGEFEAISYRSIVDKDEHVALVMGDVTTDESTLVRVHSECLTSDVFSSLRCDCGEQLNLALEMIGKEGRGVFLYMRQEGRGIGIHEKLKAYHLQDDGLDTVDANIHLGFAPDLRHYGVGVQILQDVGVTSMRIMTNNPRKLAGLSGYGELVVTERV",
    "response#2": "RMASARLPTRFGLFYLHVYEETLNGKQHLALVKGLVDGAKNVMVRVHSSCITGDTLFSLRCDCGQQLDAALKQISQCGKGVLVYLAQEGRGIGLLNKIRSYGLQDKGLDTVEANLALGFKADLREYGIGAQILADLGLSTIKILTNNPRKIVGIEGYGLKVVERVLGGEVPERPSTGLMSRLIGSDGTVRPPESRAAAEPAPVTAPDGAAVPGDVPAARPEASGATQPGASGGPVGASASGASAGPSGGALGGVPAAAPVGAPVAAPSGATPGHPSRTTAPTSVPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAPTPAAP",
    "response#3": "RMASARLPTRFGLFYLHVYEETLNGKQHLALVKGLVDGAKNVMVRVHSSCITGDTLFSLRCDCGQQLDAALKQISQCGKGVLVYLAQEGRGIGLLNKIRSYGLQDKGLDTVEANLALGFKADLREYGIGAQILADLGLSTIKILTNNPRKIVGIEGYGLKVVERVRVSEAKLPTPMGTWQLIGYRTRDHSEEHVALVMGNVGGGEPTLVRVHSQCLTGDVFSSQRCDCGEQLEIAMRHVAEERRGVIVYMAQEGRGIGLHNKIAAYALQDQGMDTVEANLALGFAADRRDYGIGMQILRDLGVHRIRLMTNNPAKRHGLSGYGLEIVERVRAEPEVVPRAVETRLPTEYGEFTAIGYRHDLDGQEHIALIAGDPAQLRGARDVVVRVHSECLTGDVFGSRRCDCGPQLQESLRKVQEAGSGVVIYLRGHEGRGIGLVHKLQAYRLQDEGWDTVDANLEQGLPEDAREYSVAGQILKDLGVESAQLLTNNPHKGEGLAGFGIEVSRHRVSEAKLPTPMGTWQLIGYRTRDHSEEHVALVMGNVGGGEPTLVRVHSQCLTGDVFSSQRCDCGEQLEIAMRHVAEERRGVIVYMAQEGRGIGLHNKIAAYALQDQGMDTVEANLALGFAADRRDYGIGMQILRDLGVHRIRLMTNNPAKRHGLSGYGLEIVERVRAEPEIVPERVAEAALPTAYGEFRAITYRTLIDAKEHQAFVMGNVDTDDPVLARVHDQCVTGDVFGSLRCDCGEQLEEAIRRVASEHRGVIVYMDQEGRGIGLHNKIRAYRLQDSGMDTVEANEALGLPADGREYGIGMQILVDLGVRKLRLMTNNPTKRSGLEGFGLEIVERV"
  },
  {
    "reference": "MGLLDGNPANETSLVLLLFADFSSMLGCMAVLIGFWRLKLLRNHVTKVIACFCATSFCKDFPSTILTLTNTAVNGGFPCYLYAIVITYGSFACWLWTLCLAISIYMLIVKREPEPERFEKYYYLLCWGLPLISTIVMLAKNTVQFVGNWCWIGVSFTGYRFGLFYGPFLFIWAISAVLVGLTSRYTYVVIHNGVSDNKEKHLTYQFKLINYIIVFLVCWVFAVVNRIVNGLNMFPPALNILHTYLSVSHGFWASVTFIYNNPLMWRYFGAKILTVFTFFGYFTDVQKKLEKNKNNNNPSPYSSSRGTSGKTMGGHPTGDDVQCSSDMEQCSLERHPNMVNNQQNLNNNYGLQQNYNDEGSSSSSLSSSDEEKQTVEMQNIQISTSTNGQGNN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have Helical; Name=1, Helical; Name=5, Helical; Name=3 domains to ensure proper insertion into the membrane.2. The protein must exhibit the following characteristics: The activity of this receptor is mediated by G proteins.; Coordinates the aggregation of individual cells into a multicellular organism and regulates the expression of a large number of developmentally regulated genes.3. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.4. Target a Polar residues compositional bias in the protein's composition for improved properties.5. The designed protein should have G protein-coupled chemoattractant receptor activity, cAMP binding, protein kinase regulator activity within cytosol, plasma membrane, cell leading edge to regulate desensitization of G protein-coupled receptor signaling pathway, MAPK cascade, aggregation involved in sorocarp development, negative regulation of phospholipase C activity, receptor signaling pathway via STAT. The designed protein sequence is ",
    "response#1": "GNLGMIVLTKLDSRLQTPMYFFLRHLAFMDLGYSTTVGPKMLVNFVVDKNIISYYFCATQLAFFLVFIGSELFILSAMSYDLYVAICNPLLYTVIMSRRVCQVLVAIPYLYCTFISLLVTIKIFTLSFCGYNVISHFYCDSLPLLPLLCSNTHEIELIILIFAAIDLISSLLIVLLSYLLILVAILRMNSAGRQKAFSTCGAHLTVVIVFYGTLLFMYVQPKSSHSFDTDKVASIFYTLVIPMLNPLISAAYSLN",
    "response#2": "RSGAWGIVLEAVAGAGIVTTFVLTIILVASLPFVQDTKKRSLLGTQVFFLLGTLGLFCLVFACVVKPDFSTCASRRFLFGVLFAICFSCLAAHVFALNFLARKNHGPRGWVIFTVALLLTLVEVIINTEWLIITLVRGSGEGGPQGNSSAGWAVASPCAIANMDFVMALIYVMLLLLGAFLGAWPALCGRYKRWRKHGVFVLLTTATSVAIWVVWIVMYTYGNKQHNSPTWDDPTLAIALAANAWAFVLFYVIPKSSQEVTEQGLQAAYVSKPGANLAKQIGASPYDDILYGVFAQSKPDSAEPMNRSAVCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEVRSDEYRTEFTTALQRVDLFMGRLNHVLLTSISTFIKGDLTIANLGTSEGRFMQVVLSRTAHFTPHVNFLLDSYPVSPEVV",
    "response#3": "GNVMVMISFKIDKQLQTISNYFLFSLAIADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYRAKRTTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFYFPVTIMCFLYWRIWRETKKRQKDLPNLQAGKKDSSKRSNSSDENTVVNHASGGLLAFAQVGGNDHDTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIKKSYTCFGSIKEWCIAWWHSGREDSDDFAYEQEEPSDLGYATPVTIETPLQSSVSRCTSMNVMRDNYSMGGSVSGVRPPSILLSDVSPTPLPRPPLASISQLQEMSAVTASTTANVNTSGNGNGAINNNNNASHNGNGAVNGNGAGNGSGIGLGTTGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGASSNAANGGGGGPGAGAAASASLSMQGDCAPSIKMIHEDGPTTTAAAAPLASAAATRRPLPSRDSEFSLPLGRRMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKRQESKAAKTLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTISTGNVMVMISFKIDKQLQTISNYFLFSLAIADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYRAKRTTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFYFPVTIMCFLYWRIWRETKKRQKDLPNLQAGKKDSSKRSNSSDENTVVNHASGGLLAFAQVGGNDHDTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIKKSYTCFGSIKEWCIAWWHSGREDSDDFAYEQEEPSDLGYATPVTIETPLQSSVSRCTSMNVMRDNYSMGGSVSGVRPPSILLSDVSPTPLPRPPLASISQLQEMSAVTASTTANVNTSGNGNGAINNNNNASHNGNGAVNGNGAGNGSGIGLGTTGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGASSNAANGGGGGPGAGAAASASLSMQGDCAPSIKMIHEDGPTTTAAAAPLASAAATRRPLPSRDSEFSLPLGRRMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKRQESKAAKTLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTI"
  },
  {
    "reference": "MSLVGGFPHHPVVHHEGYPFAAAAAAAAAAAASRCSHEENPYFHGWLIGHPEMSPPDYSMALSYSPEYRRGTANRKERRRTQSINSAFAELRECIPNVPADTKLSKIKTLRLATSYIAYLMDLLAKDDQNGEAEAFKAEIKKTDVKEEKRKKELNEILKSTVSSNDKKTKGRTGWPQHVWALELKQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The ideal protein would be one that plays a critical role in negative regulation of DNA binding, negative regulation of osteoblast differentiation, regulation of secondary heart field cardioblast proliferation, embryonic digit morphogenesis, positive regulation of transcription from RNA polymerase II promoter involved in norepinephrine biosynthetic process through RNA polymerase II-specific DNA-binding transcription factor binding, protein homodimerization activity, DNA-binding transcription activator activity, RNA polymerase II-specific, minor groove of adenine-thymine-rich DNA binding, E-box binding within transcription regulator complex, chromatin, nucleus.2. The designed protein have BHLH domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MPGMICKNSDLEFDSLKPCFYPEDDDIYFGGRNSTPPGEDIWKKFELLPTPRLSPGRALAEDSLEPANWATEMLLPEADLWSNPAEEEDIFGLKGLSGSSSNPVVLQDCMWSGFSSREKPETVVSEKLPGGCGSLAVGAGTLVPGAAAATSAGHARSGTAGVGRRKAAWLTELSHLDSECVDSAVIFPANKRESMPVATIPASAGAAISLGDHQGLSSSLEDFLSNSGYVEEGGEEIYVVMLGETQFSKTVTKLPTAAHSENAALTPECAQSGELILKRSDLIQEQHNYAAPPLPYAEDARPLKKPRSQDPLGPLKCVLRPQ",
    "response#2": "MPGMICKNSDLEFDSLKPCFYPEDDDIYFGGRNSTPPGEDIWKKFELLPTPRLSPGRALAEDSLEPANWATEMLLPEADLWSNPAEEEDIFGLKGLSGSSSNPVVLQDCMWSGFSSREKPETVVSEKLPGGCGSLAVGAGTLVPGAAAATSAGHARSGTAGVGRRKAAWLTELSHLDSECVDSAVIFPANKRESMPVATIPASAGAAISLGDHQGLSSSLEDFLSNSGYVEEGGEEIYVVMLGETQFSKTVTKLPTAAHSENAALTPECAQSGELILKRSDLIQEQHNYAAPPLPYAEDARPLKKPRSQDPLGPLKCVLRPQ",
    "response#3": "MPGMICKNSDLEFDSLKPCFYPEDDDIYFGGRNSTPPGEDIWKKFELLPTPRLSPGRALAEDSLEPANWATEMLLPEADLWSNPAEEEDIFGLKGLSGSSSNPVVLQDCMWSGFSSREKPETVVSEKLPGGCGSLAVGAGTLVPGAAAATSAGHARSGTAGVGRRKAAWLTELSHLDSECVDSAVIFPANKRESMPVATIPASAGAAISLGDHQGLSSSLEDFLSNSGYVEEGGEEIYVVMLGETQFSKTVTKLPTAAHSENAALTPECAQSGELILKRSDLIQEQHNYAAPPLPYAEDARPLKKPRSQDPLGPLKCVLRPA"
  },
  {
    "reference": "MLNTIKLGQPSFCLPTFEASIRIPSCGLHSAISTTLLAYIVIFGGIRWIYLQVSRSSQYSRIHDNEESQFDNGHLRSLRILANAIQISVGFLALSNILNIMFIFAVGERVDAEIPPYLTLSAIFYSIAWVFNYYFILYEKNNQQKWSYYTQGFWYLAFILANIELYNGLMYFIEPHDDDPSDWTQSLALISLCFIRYIINFILVICAVTLMIKRKRSYEVQGYRSISTNEDVGMDDDQTVNESDPIDTPDVGTPHIRKPISMADDPKYAPPQNIGDFLSRSKKLIPFLWPAHDIKLQVLIVICILLLVVGRIVNALLPYQNKVLVDRLTAGETIWKDILIFVGLRFLQGDVGILNTIQNLLWIPVGQFTTREISVKMFEHLLSLSLRFHHNRKTGEILRVQDRGVSSIVSILSSILFNIIPTLVDITVAIIYFTLAFDLFFGMIVFITMSLYIISTIIMTEWRTKYRRLTNQLDNAMEAKAVDSLLNYETVKLYAAEPFEVEQYTRAILDYQMADLKSNMTLYILNTAQNVTIQFGLLAGLLLCAVRISKNEMSIGDFVMYLSYILQLYAPLNWFGNYYRVIQKNFVDMEKMLDLLQEPPEIKDLPQAAPLVVKKGDVVFDNVSFSYDSKVPTLKNISFSIPSGYTVALVGPSGGGKSTILRLLFRFYDVQSGRILVDGQDIRHVRQRDLRSCIGIVPQDTVLFNDTIKYNIRYSKIGATDKQVEEAASAAQIHDKILGFSEGYETKVGERGLRLSGGEKQRVAIART",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be optimized for extracellular region, early endosome membrane-specific folding and stability, while maintaining ATP hydrolysis activity. The designed protein sequence is ",
    "response#1": "QGCGELFEKAKQNNNNGEVSSGDNGLYFSNFSLFGTPVLKDISFSIERGQLLAVAGSTGAGKTSLLMMIMGELEPSEGKIKHSGRISFCSQFSWIMPGTLKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEVFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLRPDFSSKLMGFDSFDQFSAERRNSILTETLRRFR",
    "response#2": "QGCGELFEKAKQNNNNGEVSSGDNGLYFSNFSLFGTPVLKDISFSIERGQLLAVAGSTGAGKTSLLMMIMGELEPSEGKIKHSGRISFCSQFSWIMPGTLKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEVFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLRPDFSSKLMGFDSFDQFSAERRNSILTETLRRFR",
    "response#3": "QGCGELFEKAKQNNNNGEVSSGDNGLYFSNFSLFGTPVLKDISFSIERGQLLAVAGSTGAGKTSLLMMIMGELEPSEGKIKHSGRISFCSQFSWIMPGTLKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEVFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLRPDFSSKLMGFDSFDQFSAERRNSILTETLRRFL"
  },
  {
    "reference": "MPPPKKQVKSYRSILFWIALGVILIVFWSLLQTAGTVKKEPPFSEFLIEVENDQVEEVTIAGDQINGKYIDGTSFKTTSPDNFDDLVNILREHKVSITVKSARNPWFSYLFTWFPIIILILFWVFFMRQMQSGGNKALSFGKSRAKLFSGGKKKFTFNDVAGVEEAKEELQEIIGFLKEPKRFQKLGGRIPKGVILIGAPGTGKTLLARAVAGEANVPFFSISGSDFVEMFVGVGASRVRDLFDQGKKHAPCLIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDSNEGVILIAATNRPDILDTALLRPGRFDRRIVVNMPDVKGREDILKVHIKNIPLDKKIDLSVLARSTPGFSGADLANLVNEAALLAARLDQEKVTMEDMESAKDKVLMGVERKSMIISEDEKKSTSYHEAGHALIASLLPEADPIHKVTIIPRGMALGTTQQLPLDDRYTYSKDYLEAQLSVLMGGRASEKMLLGKTTTGAANDFEKATDIARKMVCQWGMSDLGPVTFGERDDLIFLGKELAMHKNFSEKTAELIDAEVKKIINRNFKRTKELLEKHRNELINIAKALLEKEILTSKELEEIIKGKKISTDKKASLTSKVKEEKPKKEPKKIQPLKKPGLAKA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein contains novel AAA+ ATPase domains that confer a unique function or activity.2. The designed protein must have a active site active site that is highly conserved among related enzymes.3. The protein must exhibit the following characteristics: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins; Plays a role in the quality control of integral membrane proteins..4. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.5. Incorporate a signal peptide in the protein design.6. A protein that can perform zinc ion binding, ATP hydrolysis activity, metalloendopeptidase activity in plasma membrane to support protein catabolic process, proteolysis.7. The protein should be able to bind Zn(2+), ATP ligand in a variety of conditions8. The presence of Zn(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MVMTEDEKKMTAYHEAGHAIVALHEPASDPIHKATIIPRGRALGMVMRLPERDNYSYHRDKMYANLAVSMGGRVAEEIIFGYDKVSSGASSDIQYATRLARDMVTQWGMSDEMGPLQYEEPQGETFLGYSQSQRVHMSDETAQKIDKEIRRIVDAGYDRAHQLLKDHNDQLHLLANALLEFETLSGEEIKTQLRWKKRLREQGIDPDTYPLEP",
    "response#2": "MPRGVLLVGPPGTGKTLLSRAVAGEASVPYFSMSGSDFVEMFVGVGAARVRDLFRHAKKNAPCIVFLDEIDALGRRRGGSQVRTNEEREQTLNQLLVEMDGFNTDCAVVVIAATNRPDVLDPALLRSGRFDRRVSIDAPGAAELLDRIVRRDTWRERAEAGLEIARRYPWPAAAQRLTALLDGPDAPLVDTVAGLRRRLDRLGWDVDIRVIARGTPGFSGADLSNLVNEAALLAARKGKRVVTQIDFEARFGAPGFPEGGAPAPAAGAPAAGPSGPRLPSVALVDLARRRDEAGAEMARLLREHGADDARAALDALAGQARQARFPFPPLDDPAAVLREAAVRSGRTEPRTVADVLAAVRAVPGAMARVESADGARVFSAFPTPLAEHLPAGTVVTSREVPEGEAALRADVVFAVGGPRAARAAQRGAARLLVGPPGTGKTLLAKAVAGEAKVPFFSLTGSDFIELYVGVGASRVRDLFKEATKNAPCIIFIDEIDAIGRSRDSKYGGGNEEREQTLNQLLSEMDGFDTSKGILVLGATNRPEILDKALLRPGRFDRRIIVLPYLREDQERILLERFHRLLPGTGVPPDVDIRVIARGTPGFSGADLSNLVNEAALLAARKGKRVVTQIDFEGSVAAALRATLAEGAAG",
    "response#3": "MPRGVLLVGPPGTGKTLLSRAVAGEASVPYFSMSGSDFVEMFVGVGAARVRDLFRHAKKNAPCIVFLDEIDALGRRRGGSQVRTNEEREQTLNQLLVEMDGFNTDCAVVVIAATNRPDVLDPALLRSGRFDRRVSIDAPGEEALLAIVTGSEAADPDRGTAAARAADPDAMNVHGGRVVTPEAARLFAGAVAAAAAAGRDPSGCPHGRPLVLLVGPPGTGKTLLARAIAGEANVPFFTISGSDFVEMFVGVGASRVRDMFDQGKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLNQLLVEMDGFEANEGVILVAATNRPDVLDPALLRPGRFDRQVTVAMPSYEDRLAILQLHARGERLPPEVDLSLVAKSTPGASGADLANIINEAALRAVRMGRRRVTTEDLRAACNAAPRSRRDRRRQG"
  },
  {
    "reference": "MSEELFPEPGWLSEEVKTGTTIIAVTFDGGVVIGSDSRVSAGESVVNRVMNKLSPLHDKIYCALSGSAADAQTIAEIVNYQLDVHSIEVEDDPLVCSAATLVKNISYKYKEELSAHLIVAGWDKKGGGQVYATLSGLLTKQPFAIGGSGSFYINGFVDAEYKKNMTKRECQEFVVNALTLAMGRDGSSGGVAYVVTIDKDGTEEKCVLGNELPKFFDE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that contributes to proteasomal protein catabolic process, cellular response to lipopolysaccharide, response to organic cyclic compound by functioning in cytoplasm, nucleus with endopeptidase activity, threonine-type endopeptidase activity.2. The Nucleophile active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The protein must exhibit the following characteristics: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH; The proteasome has an ATP-dependent proteolytic activity. The designed protein sequence is ",
    "response#1": "LTSFVPAIMQVMLEYLTEYESKAATFLDFHAKNYSIKQENIDNARTSAVQSSSMMDTLEKCIGLLDESSMQTLYPILNRMIAKPGGVPTKIGSAQVVMLLVIRRGPLVKQFASKLLQSLKSSCFDRNAAVSDAFASAIGYLLRVCPLEIASQTCQEIIDL",
    "response#2": "LTLTIRLLRFSKPTSAITCSNDVILSLSSFYLIQKDQRIFSDLQFSQKTVDYRLSLLRWIHLSSWPSNWKWLAYFFASADSHSEVARLADEFTRDSGLPDLENLSHVNVLLDIALDKFRIEALHANFSVSISLRNKAIQHLLKSKIAANTDKAINCIEFILEAPPSMQPRLIQFTRWVVDKADPNFLKPKAAMILEKILSILSSNIIQSDLLRGFLYTTIGLLTKVDNHLITNSLLTNLLTSLQSELPDVRVSIDEALSIIIPYYSNFRFSNELLPVLEPFIFDSPESPAAYCALRFVLVAFPFDYLPARFICR",
    "response#3": "LTSFVPAIMQVMLEYLTEYESKAATFLDFHAKNYSIKQENIDNARTSAVQSSSMMDTLEKCIGLLDESSMQTLYPILNRMIAKPGGVPTKIGSAQVVMLLVIRRGPLVKQFASKLLQSLKSSCFDRNAAVSDAFASAIGYLLRVCPLEIASQTCQEIIDA"
  },
  {
    "reference": "MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGGAYTRYVDNNFCGPDGYPLECIKDLLARAGKALCTLSEQLDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFPLNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTGDFVKATCEFCGTENLTKEGATTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGEGSEGLNDNLLEILQKEKVNINIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGNFKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVRVLQKAAITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKESVQTFFKLVNKFLALCADSIIIGGAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMPLKAPKEIIFLEGETLPTEVLTEEVVLKTGDLQPLEQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have omega peptidase activity, single-stranded RNA binding, zinc ion binding and be active in host cell membrane, endoplasmic reticulum-Golgi intermediate compartment, membrane.2. The protein contains novel Nidovirus-type SAM-dependent 2'-O-MTase, AV-Nsp11N/CoV-Nsp15M domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "SANLLFKKMPWFVQKCGAIFADAWKVVEELLCSLKLTYKQIYDVVASLCTSAFTIMDYKPVFVVSSNSVKDLVDKCVKILVKAFDVFTQTITIAGVEAKCFVLGSKYLLFNNALVKLVSVKILGKRQKGLDSAFFATNLIGATVNVTPQRTESAYISLNKVDDVVTPGGGHIVIIGDMAFYKSEEYYFMMASPDSVLVNNVFKAARVPSYNIVYDVNDDTKSKMVVKIGTSFDFDGDLDAAIAKVNDLLIEFRQEKLCFRALKDGENILVEAYLKKYKMPVCLKNHVGLWDIIRQDSGKKGFLDTFNHLNELEDVKDIKIQTIKNIICPDLLLELDFGAIWYRCMPACSDKSILGNVKIMLGNGVKVVCDGCHSFANRLTINYNKLCDTAC",
    "response#2": "KVNINIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGNFKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVRVLQKAAITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKESVQTFFKLVNKFLAG",
    "response#3": "GNLVDVTEEVLGTPWFVRKLGSLFSACWQEFVEFVRSLKLHTSQLLELAKRLACASLSVVDGAFKFVVDVPTKLQCCVDKLVAMFDTVFSATCDAVRVGGKCFSKVGDYLLLDNALVTLVKAKIKGPRQAGVNHLRYASVILGPTHKVKSHRVEFYKPNLVLVDEIVPISTNGHTVVIDGLAFYCADGFYRFMADVDTVLQEPVFRNVEDLKPTFECKPIVGFPEVSASNVVDLCVKVDGLLKNYDTTYKTYSTIIKSEKCFITCKLQFTAPTYILDVQRFVDLCEAHYDDKGFHEFYTAAHEVSDLATFISQCCAMDGFDCFKPSMPICPPIFEEIDGGLIWQTFIAGLNSAIDFVKNLKVDLGLGGVVVSVSKRFKKAGAMLSQLL"
  },
  {
    "reference": "MTVLCTLMAFVMVVAISSLTVDAIPHSHESYWDQQDDIDRDEFLELLSRLSRTVMNRPEMENSKRGLDLGLSRGFSGSQAAKHLMGLAAANYAGGPGRRRRSEQA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have diuretic hormone activity within extracellular region to regulate body fluid secretion.2. The protein must exhibit the following characteristics: Stimulates Malpighian tubules fluid secretion.. The designed protein sequence is ",
    "response#1": "MGPSLSIVNPMDVLRQRLLLEIARRRLRDAEEQIKANKDFLQQL",
    "response#2": "RMPSLSIDMPMSVLRQKLSLENERKLQSLRAMANRNFLNDR",
    "response#3": "NKEAPMQQQCQSPLRQKLPPGAEAGAVLAGELGFLAQPLAAFVRLRDPVWLGPLTEVPLPSRFFCLLLGPPMLGKGYHELGRAAAVLLSDPHFQWSVRRASNLHDLLTALDAFLEEVTVLPPGRWDA"
  },
  {
    "reference": "MDTSHYRDLLARFSKARVLVVGDIYLDENVYGIVTGVSLEAPIPIYEVHERKHNPGAAGNAACNVAALGAKTSMVGYVGNDVNADIVRKEFAVRNVDTSGLVVHPTRATNTYGKLRAGGFNIPTQEILRTDTPTPVFIEGDVEDRIIANIRARAKDVDAIVVTDQVKSVATRRVLDAVVACAKEHKLLTVGDSRGRAGEFHGFDVMVPNDREAGIGTGIDVVDEETLNAAGKALLNVCKNAAVTRGQHGIRVFAADGSISDHPTTVDPASVVDVTGAGDTVTAAVAVTLVAGGSLSDAAVIGNYAAGVAVAQRGVVTVPIAQLERAMFSASGPAKLKPLDELKAELERLRAEGKRVVWTNGCFDILHVGHISYLQRAAALGDVLVVGLNSDASVRKLKGPDRPIIDEMERAFVLSALECVGLVTIFNDESPLDLIKQLKPDIYAKGGDYTIDTIVQPERRAVESYGGKIAILPAVAGRSTTSIISRIARNV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7- phosphate at the C-1 position to selectively form D-glycero-beta-D- manno-heptose-1,7-bisphosphate.2. The designed protein is the optimization of the active site to achieve high catalytic efficiency for ATP + D-glycero-beta-D-manno-heptose 7-phosphate = ADP + D-   glycero-beta-D-manno-heptose 1,7-bisphosphate + H(+).3. The protein contains novel Carbohydrate kinase PfkB, Cytidyltransferase-like domains that confer a unique function or activity.4. The protein should have a high affinity for ATP.5. A protein with heptose-1-phosphate adenylyltransferase activity, phosphotransferase activity, alcohol group as acceptor, ATP binding, heptose 7-phosphate kinase activity is needed to enhance lipopolysaccharide core region biosynthetic process, ADP-L-glycero-beta-D-manno-heptose biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "MVVGDVMLDRYWYGNTHRISPEAPVPVVHVQQQDERPGGAANVALNIRTLGAHTLLLGTTGLDEEANSLSKKLHDAKIECHFQIIKAPTTTKLRLISQQQQLIRIDFEQSSSTEHLHELLTYFDNLIQKTKLLVLSDYNKGVLRYAPQFIQKARKKNIPVIVDPKGHDFSIYRDATLLTPNKKEFEIIVGPCQTQEILVRKAKELIEQLNLTALLITEGAQGMTLVQKNLPHVHFPAHQHEVFDVTGAGDTVIATLAVAMALGYELEPAVYLSNVAAGIAVTKLGNATITFSEKIKILVVGDLILDEYIWGSVERISPEAPVQVVEVQSENLIPGGAANVANNLVALGCDVYMTGAIGKDQKGDKLIQLLQEENXNCDGIKXFDHRPTINKXRVIAHSQQIIRIDREDKRNLGDKMEKXIIAYLVSTIPMVDGVICSDYLKGLLTDNILKTIMTTSKENKKLVFVDPKGKDFLKYKGSNVLTPNEHEVKMASGENSTGNHDLTNIAKKLIDSIELDSLLVTRGKDGMCLFERDNEPIHIPTEAKEVYDVTGAGDTVIATFAMATLSGLNFLDAAKIANIAAGVVVGKVGTAVIHKEDLKKLIVSQAKSYKKTVVFTNGCFXIIHGGHIEYLQKARDLGDILIVGLNSDSSVKKLKGEGRPLKTEQERANILAALKYVNYICIFSDETPEKMIREIKPDIIAKGSDYSLEQVVGRDIVEKYGGKVELVPIVEGLSTSALVNDLKIRN",
    "response#2": "MVVGDVMLDRYWYGNTHRISPEAPVPVVHVQQQDERPGGAANVALNIRTLGAHTLLLGTTGLDEEANSLSKKLHDAKIECHFQIIKAPTTTKLRLISQQQQLIRIDFEQSSSTEHLHELLTYFDNLIQKTKLLVLSDYNKGVLRYAPQFIQKARKKNIPVIVDPKGHDFSIYRDATLLTPNKKEFEIIVGPCQTQEILVRKAKELIEQLNLTALLITEGAQGMTLVQKNLPHVHFPAHQHEVFDVTGAGDTVIATLAVAMALGYELEPAVYLSNVAAGIAVTKLGNATITFSEVIGDAMLDTYFEGTAARLCSEGPVPIVRKTSERHLPGGAANTAANLRALDAQVMLLGFVGRDNAGNLLRAALRERGIDDTWLIEDERASTLHKLRILADGQFVVRFDDEDAHHYAPPGQQLLLARLEEMFPRCDVVIVSDYCYGVISDALIDRLQFLHAAHPNVLLAVDSKNLRRFKHAGATVITPNLLEAQLLVTQNDDMQGLLPAVPEQASTPFPEAERIGQRLLSMIDAEHAVITMAADGVMLVDRQGRARHIPAHPVHHANDIGAGDSFVAAMALSLAAGAQLDEAARIGIDAAEIAVTKRWDVERQRGRTIVFTNGVFDILHAGHVHLLRQAKQLGDVLVVGVNSDQSAQRLKGKHRPINSERDRLALVAALDPVDYVALFDEDTPATLIRTLRPHIHVKGGDYADEQLPEAGAVHEVGGRIVIIPLVGSMSTSSVIDRIL",
    "response#3": "MVVGDVMLDRYWYGNTHRISPEAPVPVVHVQQQDERPGGAANVALNIRTLGAHTLLLGTTGLDEEANSLSKKLHDAKIECHFQIIKAPTTTKLRLISQQQQLIRIDFEQSSSTEHLHELLTYFDNLIQKTKLLVLSDYNKGVLRYAPQFIQKARKKNIPVIVDPKGHDFSIYRDATLLTPNKKEFEIIVGPCQTQEILVRKAKELIEQLNLTALLITEGAQGMTLVQKNLPHVHFPAHQHEVFDVTGAGDTVIATLAVAMALGYELEPAVYLSNVAAGIAVTKLGNATITFSERALLVGDFMLDEIVHGDAGRLSPDAPVPVLKAEHEEQSPGGAAHVARCLAALGGDVICCGVVGADAAADQLRAALVEQGIDAEGLLTDSTRPTTVKRSLVGLAQHRHPQKMFRLDIESKAPLDDALEQSLISMIKARLDGVDVICIEDYDKGVCTPRVCQAVIELARDSGVEVLVDPAPLEDYTKYRGATSITPNRTEAELATGAPASTVEAANGLAEQLECEAVILTLDKDGALLLVKGESPAVIPTEARSVYDVTGAGDVVISALAAARANRMGWHDATRFANAAAGLEVACFGTRPIPLAEVQAHFAKRQASGQGLATRWRASGLRVGFTNGCFDILHPGHLRVLRQARAQCDRLIVAVNTDASVKRLKGESRPVNTEQDRAELLSGIAAVDAVTSFDEDTPAELIEALQPDVLVKGGDYKADEIVGADTVRARGGEVIIVPLLEGRSTTNIIERL"
  },
  {
    "reference": "MSEPSSCFVLPDPEWIYRVGIGQDSHRFLPSDNPKPCVLGGIIFENTPGFEANSDGDVVFHAICNAFSSVTHQVILGALADELLKAKGISDSAVYLKEAVASLKPTQKISHLAITIEGQRPKLLPKISAMREKIAEILRISLDSVNITATSGEGLTSMGQGHGVQCFCVLTVMEFCPR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be able to regulate terpenoid biosynthetic process.2. We require a protein that can specifically bind to the metabolites produced in the isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6, isopentenyl diphosphate biosynthesis via DXP pathway pathway.3. The protein must exhibit the following characteristics: Catalyzes the conversion of 4-diphosphocytidyl-2- C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).. The designed protein sequence is ",
    "response#1": "EAGDFSRAAKQLRGNMDVRLGNGFDVHAFEDGDHVMLCGVAVPHTHKLKGHSDADVGMHAVTDALYGALAEGDIGRHFPPSDPKWKGAESAIFLRHAVNLARDRGYAIGNIDLTLICERPKIGPHAAEMTSKMADIMDLDSSRISIKATTSEQLGFTGRKEGIAALATATLV",
    "response#2": "TDDASIAEWAGMPVTLVEGSPDNFKLTTKRDIAMADQILSTLPDIRTGNGYDVHQLVEGDGVTLCGIFIPHDQKLLGHSDADVALHALTDALLATCGAGDIGDHFPPSDPQWKGAPSRIFLEHAAKIVRERGGVIMNADISLIAEAPKVGPHRDRMRANLSEMLGISLDRCSVKATTNEKIGFIGRREGIAAIATATG",
    "response#3": "EANLKITTEADFARAEKELRGDMDVRTGTGFDVHAFAEGDYVILCGVKVPHDRTLKGHSDADVGLHAVTDAIYGALADGDIGRHFPPSDPKWKGAASHVFLTHAAGRAAERGYRLSNVDCTLVCERPKIGPHAAAMAGEIARLVKVAPERVSVKATTSETLGFTGREEGIAALATVTLFE"
  },
  {
    "reference": "MKFFRLFSKPLFTVSTSGNSGMRDILINKLKKIDKNNKEKLVSQIFSDVSDKYDIMNDLMSFGLHRIWKNNLVDLIKLSPEKVILDLATGSGDIIKIIKKRSECLCLGYDSNLDMINEAKKKNLKNAFFVCGKSEEMPFSKNTFDYVIVSFGLRNFSDMDKSLKEVFRILKPSGKFLCLEFSQINNPLLSKLFNIYNKIIPKYGKFLSKNELAYKYLIKSIEIFPNQIKLSKKLLKSGFRKIEVFDIIDGLSSIHVAKK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be optimized for 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity, demethylmenaquinone methyltransferase activity, S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity activity in order to improve methylation.2. A protein should be able to manipulate the activity of enzymes in the Quinol/quinone metabolism pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "GLLAKLL",
    "response#2": "GNDSTR",
    "response#3": "MFAVIKTGGKQVKVS"
  },
  {
    "reference": "MNREDFIEEKVFYIEEEEVKDSEKKEKPQEDEKKTTQEPDNKKPQEDEEKKNEEKEKIHDEIKERKEELKSKLRDGLNQSSNKSEEDRNNKLKSLGGKFNFKGFVMLLFIVTLIASAPALLSTNAKTPSNEVGYSEFINHVKNKEIVKVNEKEGYVYGYSPEDEKKEVKSYKARMITDRLGDDPVLVKTIEENNASIKSLPPQELPFLLNMLASWFPMLLLIGVWIFMLNRMNKGSGGGPQIFNMGKSKAKDNGEEISKVTFDDVAGIAEAKVELEEVVKFLKEPETFKKIGARIPKGVLLLGGPGTGKTLLAKAVAGEAKVPFFSMSGSEFVEMFVGVGASRVRDLFNKARKSAPCIIFIDEIDAVGRKRGSGQGGGNDEREQTLNQLLVEMDGFGTDETIIVLAATNRPEILDKALMRPGRFDRQVIVDNPDIKGREEILKVHIRGKKIAKDVDLSIIAKKTPGFVGADLANLLNEAAILAAREGREEITMADLEEASEKVSIGPERKSKVMIEKERLITAYHEAGHALMHYLLPNTDPVHKITIVPRGMAGGFTMALPEEERSYKFKSEFFDDIRVLFGGRAAEQIVFNDITTGASNDIERATAIAHAIVTRFGMTNKFGPMLLDNTKEGDLFQQKYYSDTTGKEVDDEIRGIISTAYTETLDMIKKNYQYLDNVAKALLEKETLVREEFEAIMQGKTLADLAESKKEAAEIKADEAVEEKEKEDIAEKIKADDAVRVLDEFEEKEEEKFSKD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity.3. For general function, the protein need meet that Plays a role in the quality control of integral membrane proteins.4. The protein should be able to bind Zn(2+), ATP ligand in a variety of conditions5. The designed protein should have ATP binding, zinc ion binding, ATP-dependent peptidase activity, ATP hydrolysis activity, metalloendopeptidase activity within plasma membrane to regulate cell division, protein catabolic process, proteolysis.6. Include a signal peptide as an essential component of the protein.7. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "IPKGVLLVGPPGTGKTLIARAVAGEASVPFYFVSGSDFLEMFVGVGASRVRDMFRVAKQNAPCILFIDEIDAVGRQRGTGLGGGHDEREQTLNQLLVEMDGFGHNSGVIVLAATNRVDILDPALMRPGRFDRQIYVSRPLNFDVDLDIIAAAAVGLTGADIRNIVNEAALWAARNDKSVVSMADFINAVIPQADLREAEGRLDYDRLADALEAWW",
    "response#2": "VLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFENAKKTAPSIIFIDEIDAVGRQRGAGLGGGNDEREQTLNQLLVEMDGFQDDGNSVIVIAATNRSDVLDPALLRPGRFDRKVLVVQMNVDLKVVAKGTPGLVGADLANLVNEASLLAARNGKSKVSMEDFEKFLEKAILPLPEGVTADLVLSEFEKKLVAYHEVGHAMVAYRQKHAEPVSKITIVPHTQGALGYTLHLPEEDKHLHTRDELLAQITVSMGGRVAEELVLHTMTNGAAQDIQDATGIARNMVAMFGMSDEFGMMALASRRSQYLDGGYGIDCAQDTAAAMDRAVRDLIDACYKKAVEILSHSREDMDKVVEYLLEKETITGAEMVRLMGYGIRATTLIQDSDT",
    "response#3": "VLLVGPPGTGKTLLAKAVAGEANVPFFSMSGSDFVEMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAIGRARGRNVNFSANDERENTLNQLLTEMDGFASNQGVIILAATNRADILDRALMRAGRFDRQIHVLFIDCPDEELERRLLGRGREDKLERGEINWYLQQAQPFQRFAEAAYDLTIDTTNTTPEESARQVLEILQTRFGFAE"
  },
  {
    "reference": "MASISSLHRWASNQHSRLPRITSISEADQSRPINQVVAFSVPISRRDASIILLSSIPLTSFFVLTPSSSEARERRSRKVIPLEEYSTGPEGLKFYDIEEGKGPVATEGSTAQVHFDCRYRSITAISTRESKLLAGNRSIAQPYEFKVGSTPGKERKREFVDNPNGLFSAQAAPKPPPAMYFITEGMKVGGKRTVIVPPEAGYGQKGMNEIPPGATFELNIELLRVTPPPEEK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides ..2. A protein that localizes to nucleus, thylakoid lumen, chloroplast, chloroplast thylakoid lumen and can perform peptidyl-prolyl cis-trans isomerase activity with high specificity and efficiency.3. The designed protein must have at least one PPIase FKBP-type domain.4. The protein should have a fast turnover rate for [protein]-peptidylproline (omega=180) = [protein]-   peptidylproline (omega=0). The designed protein sequence is ",
    "response#1": "LRRAIPANPSMKIIQASLEDISYLLRIPQRKPYGTMESNVKKALKVAIDDKDKILASIPVDLKDKGSELYTTLIDGKGGLQALITSIKKQDPDKVSLGLAASLDTVADLA",
    "response#2": "LRRAIPANPSMKIIQASLEDISYLLRIPQRKPYGTMESNVKKALKVAIDDKDKILASIPVDLKDKGSELYTTLIDGKGGLQALITSIKKQDPDKVSLGLAASLDTVADLI",
    "response#3": "IVLDGYNAPVTAGNFLDLVERHFYDGMEIQRRDGFVVQTGDPEGPAEGFIDPSTEKPRTIPLEIMVEGEKVPVYGSTLEELGLYKAQTKLPFNAFGTMAMAREEFENNSGSSQIFWLLKESELTPSNANILDGRYAVFGYVTDNQDYLADLKVGDVIESVQAVSGVDNLVNPTYKIAK"
  },
  {
    "reference": "MRTVLGVAGGIAAYKAAHVIRRLRELGHSVKVVPTASALNFVGAATFEALSSQTVTTDVFDDVESVNHVRIGQEAELVIIAPATANILAKLAAGIADDLLTATVLTTTAEVVVAPAMHTEMWQNPATVANIATLKQRGIHVLDPAVGRLTGPDSGPGRLPEPQTIVDFALAAAGSAATAVGPLNDKRVVISAGGTREPLDPVRFLGNRSSGKQGIALARAAVAAGARVDLVAANIDWGLLSALPDGVRITTVESTLELQTQMHAVQADADVIIMAAAVADYRPAGRTDSKMKKSGDEGMTLSLVQNPDVLVGLVESRREPQVIVGFAAETGDSHHTALDYARAKFRRKGVDLLVFNNVSEDRAFGHDDNAVQILSHDGDQVVTSTEFHGSKDDVSEKVIEALANRMECVESSP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein have DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains with specific sequence motifs that are crucial for its activity.2. The designed protein must be able to regulate pantothenate catabolic process, coenzyme A biosynthetic process.3. The CTP binding site should be stable and able to withstand changes in environmental conditions.4. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "MTQSATHFISPLTFQALSGNPVCTTLLDTDAENAMGHINLARWADLFLIAPATANVLAKCSHGLADDLVSTLYLAATCPIVMAPAMNQAMWHKTVTQENVQTLIRHGVTLMGPGQGTQACGEMGFGRMSEASEICDFLT",
    "response#2": "MTQSATHFISPLTFQALSGNPVCTTLLDTDAENAMGHINLARWADLFLIAPATANVLAKCSHGLADDLVSTLYLAATCPIVMAPAMNQAMWHKTVTQENVQTLIRHGVTLMGPGQGTQACGEMGFGRMSEASEICDFLE",
    "response#3": "MTQSATHFISPLTFQALSGNPVCTTLLDTDAENAMGHINLARWADLFLIAPATANVLAKCSHGLADDLVSTLYLAATCPIVMAPAMNQAMWHKTVTQENVQTLIRHGVTLMGPGQGTQACGEMGFGRMSEASEICDFLE"
  },
  {
    "reference": "MSVTRLSEPLQNILLQDLRNFYDRASRIATLSVSAIAAIKSAWTRGSPFAAATALYPTNEEGKYVIQAEGIRMEFTNYGGAVTNLWLNNSRGEEVDIVLGLDHARDYEDYPKNPYLNGAIGRYAGFMRGGRFDMDGESYQVATNAHNGSSTFNGGDRGWGRSILDIGSHTENSITFVLFDRSWNGFPGTAASCLTHTVTPYEWRVAFGVTPTKKPGPINMSQQAFFNLDGFKKKNLTGSVPVSDKTVRDHKLHLPLSGLRFETDALGLSTGDILGNPRGSEYDFWSASRRIGDVLEKPYMGICDRCQKRQYHNHNPSGAYDTIFQLGRSQPWNKEDVPAAILSSPESGISMKLYSDQEALHVHTWSQKEFPLKLKKGQGQGMVPQHGGISFEMQDWPDGLNHPEWRRESKTIWGMDGLYTAFSSYRFSVDKTEP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should consider the endogenous proteins and enzymes present in the Secondary metabolite biosynthesis pathway to avoid interference with native metabolic processes.2. For general function, the protein need meet that Fusarielins F, G, and H have a C11=C12 cis double bond and is fully reduced between C10 and C11 and between C12 and C13; Epimerase; part of the gene cluster that mediates the biosynthesis of fusarielins F, G and H, decaketide compounds with 5 methylations and a decaline core that act as mycoestrogens as they stimulate growth of MCF-7 breast cancer cells; FSL3 can be involved in the formation of the C11=C12 cis double bond by moving a hypothetical C10=C11 or C12=C13 trans double bond to form prefusarielin; The final step in the pathway is a reduction of the carboxylic acid moiety to yield fusarielin H via a still undetermined mechanism.; The initial compound in the pathway is produced by the reducing polyketide synthase FSL13. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.4. A protein with carbohydrate binding, isomerase activity functions is required to enable carbohydrate metabolic process.5. The designed protein should have a high specificity for substrate binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "IAIIGTGCRFPGGSNTASKLWDLLKDPKDVSKEVPEDRFNLDRFYHKDSSHHGTANVRRSYLLDEDVRLFDTQFFGISPGEAQAMDPQHRVLLEVVYEAIESAGKTIHGLHNSDTAVYVGLMCTDYYVIQAADLNSVPTYNATGVANSNASSRVSYFFNWHGPSMTIDTACSSSLVAVHEAVQALRNGTSRMAVACGTNLILSPLPFISESNLSMLSPTGKSRMWDADADGYARGEGVAAVVLKPLSAAIEDNDVIECIIREVGVNQDGKTRGITMPSAQAQASLIRQTYAKAGLDPATPEGRCQFFEAHGTGTPAGDPQEAEALKTAFFPNETDSVTNGTNGLLSEADNLLVGSIKTVIGHTEGTAGLAGLIKACMALKHGAVPPNLLFNRLNPALEPFTKHLSIPTSLTPWPTLLTNVPRRASVNSFGFGGTNAHAILEAYSQHNYAYPKHSKPSQLEPIAIIGTGCRFPGGSNTASKLWDLLKDPKDVSKEVPEDRFNLDRFYHKDSSHHGTANVRRSYLLDEDVRLFDTQFFGISPGEAQAMDPQHRVLLEVVYEAIESAGKTIHGLHNSDTAVYVGLMCTDYYVIQAADLNSVPTYNATGVANSNASSRVSYFFNWHGPSMTIDTACSSSLVAVHEAVQALRNGTSRMAVACGTNLILSPLPFISESNLSMLSPTGKSRMWDADADGYARGEGVAAVVLKPLSAS",
    "response#2": "IAIIGTGCRFPGGSNTASKLWDLLKDPKDVSKEVPEDRFNLDRFYHKDSSHHGTANVRRSYLLDEDVRLFDTQFFGISPGEAQAMDPQHRVLLEVVYEAIESAGKTIHGLHNSDTAVYVGLMCTDYYVIQAADLNSVPTYNATGVANSNASSRVSYFFNWHGPSMTIDTACSSSLVAVHEAVQALRNGTSRMAVACGTNLILSPLPFISESNLSMLSPTGKSRMWDADADGYARGEGVAAVVLKPLSAAIEDNDVIECIIREVGVNQDGKTRGITMPSAQAQASLIRQTYAKAGLDPATPEGRCQFFEAHGTGTPAGDPQEAEALKTAFFPNETDSVTNGTNGLLSEADNLLVGSIKTVIGHTEGTAGLAGLIKACMALKHGAVPPNLLFNRLNPALEPFTKHLSIPTSLTPWPTLLTNVPRRASVNSFGFGGTNAHAILEAYSEYVQKVPKPALPRLVFIFGDQTLDVQPCLRGLAQHRHNPVLEDFFTKTYQVLRTEIDQLPHNVKVGLPRFTCIDDIIFRPPGSKSCIALDMAMTTFYQLGVFIRVTCALGLCTGALAAAAVSCSRNALDLVPMAVDAVRVAFRTGAHVLDIAHRIESPDASDQSWLLYVPAVEAAETALKAFREHTLLPGIRQPYITAYTLNSVMVSGPPSSLEQLKRHAPFRELNPQSVSINGPYIPIIPPLTGISVDVPVGKLTLVVGPVASGKSTFCKALLGEVPFSSGEVIVPSTEAPIGYCEQTPFLKNGSIRDNIIWHSVYNQTRYEEVLDACLLRTDLDILPEGDATTIGSNGIMLSGGQKQRVSLARALYLETDFLLID",
    "response#3": "IAIIGTGCRFPGGSNTASKLWDLLKDPKDVSKEVPEDRFNLDRFYHKDSSHHGTANVRRSYLLDEDVRLFDTQFFGISPGEAQAMDPQHRVLLEVVYEAIESAGKTIHGLHNSDTAVYVGLMCTDYYVIQAADLNSVPTYNATGVANSNASSRVSYFFNWHGPSMTIDTACSSSLVAVHEAVQALRNGTSRMAVACGTNLILSPLPFISESNLSMLSPTGKSRMWDADADGYARGEGVAAVVLKPLSAAIEDNDVIECIIREVGVNQDGKTRGITMPSAQAQASLIRQTYAKAGLDPATPEGRCQFFEAHGTGTPAGDPQEAEALKTAFFPNETDSVTNGTNGLLSEADNLLVGSIKTVIGHTEGTAGLAGLIKACMALKHGAVPPNLLFNRLNPALEPFTKHLSIPTSLTPWPTLLTNVPRRASVNSFGFGGTNAHAILEAYSSHHHHGPGHQPPRPFIFGDQTLDVQPCLRGLAQHRHNPVLEDFFTKTYQVLRTEIDQLPHNVKVGLPRFTCIDDIIFRPPGSKSCIALDMAMTTFYQLGVFIRVTCALGLCTGALAAAAVSCSRNALDLVPMAVDAVRVAFRTGAHVLDIAHRIESPDASDQSWLLYVPAVEAAETALKAFREHTLLPGIRQPYITAYTLNSVMVSGPPSSLEQLKRHAPFRELNPQSVSINGPYARDEPDRVLFYTPRNGQPSQGYDEVNTKIFANSINRLCGWLDSQLGSPAGPRTIAYIGQNDLRYFIMMIASTKLGHRLLLSSPRNSVEGHVSLIKQSGCEFWIASSGLDHHEFLQDLQIPSVEAPELPQLLDPTPVKPYVYQKSWNEGKSDVLALLHTSGSTGLPKLVPVYLETAATVDGFHLMEPTNGKRPTGVEWTGTRQLCAMPLFHVAGICLGLYSAVFFNWTVVLPSVGPIMQHVIEDALDHISLDSAFISPSVLQDISKSPRVLEKLSKLKFITSAGGPIPQSVGDLIHPRVPIMQTMGMTEGQWLASVVTHPDEW"
  },
  {
    "reference": "MSTLPPLYATLNVALGGAIGAVLRYQMGRWMTGWLGAPAMSVFPWATLAINALGSLLMGVLAGVLFKLSPGVQDQWRLLIGTGILGGFTTFSAFSLEVWVMVERGQPAFAALYVVLSVSLAISALVFGLMLTRLFA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Important for reducing fluoride concentration in the cell, thus reducing its toxicity.2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.3. A protein that localizes to plasma membrane and can perform fluoride transmembrane transporter activity with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "MNKMATLKK",
    "response#2": "MITSN",
    "response#3": "MNQ"
  },
  {
    "reference": "MISVNEYFEGNVKSLGYLSAEGKSTVGVIDKGEYKFGTSTDETMLVIEGELEVLLPEADKWITVKSGEDFKVPANTSFEVKAEVPTSYLCKYE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must have high specificity towards uridine phosphorylase activity.2. For general function, the protein need meet that Also catalyzes the reverse reactions. The designed protein sequence is ",
    "response#1": "MLTITFFWVRL",
    "response#2": "ECRIL",
    "response#3": "GFMSL"
  },
  {
    "reference": "MNQKVDIEALHETSINSDQPLLRLQQVSRSFMAGDREFQVLKHIDLAIHTGELVAIIGASGSGKSTLMNILGCLDHASAGSYQVNGQETRELDDDALAALRRDHFGFIFQRYHLLPHLDAVRNVEIPAIYAGTAQTTRHERAQALLTRLGLGGHLQHRPSQMSGGQQQRVSIARALMNGGQVILADEPTGALDTASGKEVMRTLLELHAAGHTVILVTHDPKVAANAERIIEVSDGEIISDRRTAQTTQPAPEAQPATPPGPAPRRLLASLGLFREAFNMAWIALISHRMRTLLTMLGIIIGITSVVSISAIGEGAKRYVLKDIQSIGSNTIDIYAGANFGDSRAKSIETLLPSDVAALNQLYYIDSATPVVGRSMLVRYRNVDVDAQLNGVSSRYFQVRNIQLAAGITFSDQDARRQAQVVVLDHNTAQRLFGPGVNPLGQVILVGKLPCTVIGVTSDHKNLFIAGNTLNLWMPYETAAGRVLGQRHLDSISVRVKDGMPSKAVEEQIKALMLQRHGTKDFFTNNLDSVMQTVQKTSRSLTLLLSLIAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARQSDIRQQFLVEAVMVCLMGGVIGIGLSYAIGYLFTLFVQQWEMVFSLASVVTAFACSTLIGVLFGFVPARNAARLDPIEALARD",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Part of the tripartite efflux system MacAB-TolC2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The designed protein have ABC transporter domains with specific sequence motifs that are crucial for its activity.4. The designed protein should be involved in xenobiotic transport by localizing to plasma membrane and exhibiting ATP binding, ATP hydrolysis activity. The designed protein sequence is ",
    "response#1": "LSFKDLTLTLKSNGKQVLRCVTGSMKPGRITAVMGPSGAGKTSLLSALAGKAVGCKLSGLILINGKQESIHSYKKIIGFVPQDDVVHGNLTVEENLWFHAKCRLPADLSKADKVLVVERIIDSLGLQAVRSSLVGTVEKRGISGGQRKRVNVGLEMVMEPSVLFLDEPTSGLDSASSQLLLRALRHEALEGVNICMVVHQPSYTLFKTFNDLVLLAKGGLTVYHGSVNKVEEYFSGLGIHVPT",
    "response#2": "CVTGKLSPGRVTAIMGPSGAGKTTFLNAVLGKTTGYKKDGLVLINGKSGSMQSYKKIIGFVPQDDIVHGNLTVEENLWFSACCRSSKGMSKSDKIIVLERVIGSLGLQEIRNSLVGTVEKRGISGGQRKRVNVGIEMVMEPSLLILDEPTC",
    "response#3": "PGRITAVMGPSGAGKTSLLSALAGKAVGCKLSGLILINGKQESIHSYKKIIGFVPQDDVVHGNLTVEENLWFHAKCRLPADLSKADKVLVVERIIDSLGLQAVRSSLVGTVEKRGISGGQRKRVNVGLEMVMEPSVLFLDEPTSGLDSASSQLLLRALRHEALEGVNICMVVHQPSYTLFKTFNDLVLLAKGGLTVYHGSVPGRVTAIMGPSGAGKTTFLNAVLGKTTGYKKDGLVLINGKSGSMQSYKKIIGFVPQDDIVHGNLTVEENLWFSACCRSSKGMSKSDKIIVLERVIGSLGLQEIRNSLVGTVEKRGISGGQRKRVNVGIEMVMEPSLLILDEPTTGLDSASSQLLLRALRHEALQGVNVCAVIHQPSYTLFNMFDDFVLLARGGLIAYLGPIPGRITAVMGPSGAGKTSLLSALAGKAVGCKLSGLILINGKQESIHSYKKIIGFVPQDDVVHGNLTVEENLWFHAKCRLPADLSKADKVLVVERIIDSLGLQAVRSSLVGTVEKRGISGGQRKRVNVGLEMVMEPSVLFLDEPTSGLDSASSQLLLRALRHEALEGVNICMVVHQPSYTLFKTFNDLVLLAKGGLTVYHGSVQEIREQDVNLDIKPGQLVAVVGTVGSGKSSLVSAMLGEMENVHGHITIQGSTAYVPQQSWIQNGTIKDNILFGSEYNEKKYQQVLKACALLPDLEILPGGDMAEIGEKGINLSGGQKQRVSLPGRVTAIMGPSGAGKTTFLNAVLGKTTGYKKDGLVLINGKSGSMQSYKKIIGFVPQDDIVHGNLTVEENLWFSACCRSSKGMSKSDKIIVLERVIGSLGLQEIRNSLVGTVEKRGISGGQRKRVNVGIEMVMEPSLLILDEPTTGLDSASSQLLLRALRHEALQGVNVCAVIHQPSYTLFNMFDDFVLLARGGLIAYLGPI"
  },
  {
    "reference": "MSEAKRRAAEKAIEYVENDMIIGVGTGSTVAYFIDALGHTPKRIKGAVSSSEQSTAHLKQHGIEVLELNHTGTLPLYVDGADECDPYKRLIKGGGASLTREKIIAEASKQFICIIDPNKQVATLGKFPLPIEVIPMARSLVARQIMARTDGQPVWREGVITDNGNVILDVHHLCITDPVKLEQELNQIPGVVCVGLFARRCADLVIIGSEPPHIL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.. The designed protein sequence is ",
    "response#1": "GAPEGL",
    "response#2": "GFATNPRV",
    "response#3": "MTPLDV"
  },
  {
    "reference": "MAERRRLVIASGNTGKLREYRELLAGTGLDLVAFDGDVDEVGETYAENARLKAETAAGRSGLPALGDDSGVEVEALGGFPGIRSARLGPTQQDRTAELLRRLEGKPRPWNARFVCVVALADRGSETRFFEGECRGEIVPEWRGAAGFGYDPVFLVPGTGKTFGEMPPEEKRLYSHRARAVRALLESGALEGLSV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design must be able to perform ITP diphosphatase activity, dITP diphosphatase activity, metal ion binding, ribonucleoside triphosphate phosphatase activity in order to contribute to nucleotide metabolic process, purine nucleoside triphosphate catabolic process.2. The Proton acceptor of the protein must be engineered to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+).3. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..4. The protein should be able to bind substrate, Mg(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MMLDATGIDTAHPVETTLACAPGRLAYLCGNSLGALPRSAPGIIEEELDRWAELGVEGHFHGRSPWLSYHELLTDDSAALSGQLVGAAAGETAVMNSLTVNLHLLLVSFYRPEGQRHRILLEAGAFPSDQYAVHSQLRFHGFEPGEAIIEVARRPGEATLRTEDILEAIDRHAEDVALVMLGGVNYYTGQVLDMEAITRAAHQVGALAGWDLAHAAGNVPLALHDWGVDFAAWCTYKYLNSGPGAVAGAFVHERYHGERDLPRFAGWWGHNASDRFLMAPGFIPMPNADAWQLSTPPVLALAPYRASLELFAEAGMPRLREKSLRLTGYLEWLINRELGEWIEVITPADPARRGCQLSLRVRQAGRPLFEYLTGSGVLGDWREPDVIRLSPVPLYNRFEDVYLAGRRLRAAVTASAG",
    "response#2": "MKPIHVAIIMDGNGRWAKNRGKPRIFGHKAGAESVRKVVRFALKIGIKALTLYAFSSENWKRPKEEVKFLMKLFIYALKKEVKSLHNNKVRLRAIGDRSRFNKKLLKKIKKTERLTLKNKKLTLNIAANYGGRWDIVQGVRNLAKQVRDKKLKAKDINEKLFRKYLATGNLPPVXPWQSSYAEFLIWQSIYLEFWFTDIYWPTFRKEHLLRAINYYQKRKRKFGKT",
    "response#3": "MQDTETAAVVFSGGQDSTTCLFYAKKNFDKVYTVTFDYNQRHAIELEVAESVARKLGAKHHVLDMSVLNRLSNTALTANALKVPAGHYEDGTPNTFVPGRNILFLTLAGIYAYQVGAKHIVTGVCETDFSGYPDCRDIFVKSLNMAISLGMDKDLEIITPLMWLNKAQTWALADEMGALDLVRRHTVSCYQGIKGDGCGECPACHLRKRGLEDYLQNKNAA"
  },
  {
    "reference": "MINPNPKRVLIGICGGIAAYKVCEVVSSIFKAEVQVKVILTASGREFITPLTVATLSRHPAYTDADFWQPIHTRPLHIELGEWADLMVIAPLTANTLGKLAYGLADNLLTNTVLASHCPILLAPAMNTDMWEQVAVQRNWQQLLTDRRYHSVGPGAGLLACDRTGTGRMAEPNQIVTAIASLLHTNGKRDLIGKKVLISAGGTREYLDPVRFIGNPSTGKMGIALAQAALSRGASVTLVHGVLSAGISELGNPQLQAIPVVSAAEMQQALLSEFTTSDITIMCAAVADVKPSVYSAEKLPKKSLPESLSLAPVPDIVAGLGSIKKPHQKLIGFAAQTGDIITPATEKLIRKKLDAIVANPVDEIGSGFGSDRNRAIIISSSGKQQEIAPCSKLEMAHHILDFLPLL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have phosphopantothenate--cysteine ligase activity to facilitate pantothenate catabolic process, coenzyme A biosynthetic process.2. The protein must bind to Mg(2+) in order to perform its enzymatic function.3. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.4. The protein should be able to bind CTP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "SVVLGIGGGIAAYKAAELCRALVQRDFSVQVVMTAAAQEFVRPLTFAALTGRKVITGLFSSESSEATLSSAVEHIGVAQEHELLVVAPATAHLLAKFSHGLADDFLTTMYLAFTGPVILAPAMNSNMWLHPATQANLAALQARGHRIVEPGEGFLACGMEGPGRLAEPEEIAQAVLQLVRTHFHQG",
    "response#2": "MRIIVGVAGGIAAYKACEVVSRLVKAGHSVRVVMTPAAAQFVAPLTFRALSGHAVGIEAADEPAGPLSHVQLAHWAEALVIAPATASLMSRLAHGQTDDLLGLVYLGIRGPVVAAPAMEPEMWSHPRTQANASVLRADGVRLVGPAVGRMASGQEGLGRMAEPAEIVAALEDALA",
    "response#3": "DKRILLIVGGGIAAYKCLDLVRRLRERGAKVTPVMTRSAAEFVTPLSIGALAGTKVYGELFDRDDEEDVGHIRLARGADLVIVAPASAARMGRMAAGIGDDLAGAVLLATRAPVLLAPAMNPAMWTHPATRRNVETLARDGIRFVGPNRGEMAESGEAGEGRMAEPLEIAAAAEAVLGEARGPLAGRHVLVTSGPTHEPIDPVRYIANRSSGRQGHAIAASLARLGARVTLVSGPVALPDPPGVSTVHVETGREMLGAVEAALPADAAVFVAAVADWRTQDDAAHKIKKTGDAPPALRLVENPDILRTVGTGPRRPALVVGFAAETRDLLENAAAKLARKGADLIVANDVSGEVMGGEANTVHLVSRSGVEAWPTLSKTEVAERLALDIARRLGPSGGA"
  },
  {
    "reference": "MARNVLYPLYQLGGPQLRVFRTNFFIQLVRPGTAQPEDTVQFRIPMEMTRVDLRNYLEQIYNVPVAAVRTRVQHGSNRRRDHKNVRIKKPDYKVAYVQLAHGQTFTFPDLFPEKDPRSPEPLEEELPQQRQSSDLRCPGIPSWFGL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should be able to enhance the efficiency of translation. The designed protein sequence is ",
    "response#1": "DETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAC",
    "response#2": "YDCYANLALLKLYQFNPHLSRDETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAKGTVVREC",
    "response#3": "YDCYANLALLKLYQFNPHLSRDETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAKGTVVREC"
  },
  {
    "reference": "MPERIVLATNNPKKLAELRRIVAACGLAVEVSGLRDFPAYPEPEETERTFEGNAFLKATAAARETGLVAVADDSGLAVDELNGMPGVRSARWAGPACDDEDNNALLLAQLDGVPPSRRGARFVCALAMVSPDGERRVWHGSMPGHIAEAPAGVNGFGYDPLFVPAGLSVSSAELTAEEKDSISHRGIAVRAFVDYLRGLS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein with metal ion binding, ITP diphosphatase activity, XTP diphosphatase activity, nucleotide binding, ribonucleoside triphosphate phosphatase activity functions is required to enable purine nucleoside triphosphate catabolic process, nucleotide metabolic process.2. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.3. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+).4. The designed protein should have a high specificity for substrate, Mg(2+) binding, with minimal off-target effects.5. The presence of Mg(2+) is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "MKIGILVFPGSNCDVDLYRALKNELGAEAVYIHFDKDLPSDLDAIILPGGFSYGDYLRCGAIARFSPVMQAVIAFGQRGGRVLGICNGFQILTEAGLLPGALMRNRGLKFICEPLKIAVQNNQTPFTSPYSQGETITIPISHGEGNYFADQDTMEKLAKNNQICFSYVNNKGSVNDIAGICNERGNVLGMMPHPERASEDLLGGVDGLKLLKSILG",
    "response#2": "MNGDARPKIAAKLSSLSELIAMGRSPELGEILSTDAHIISAPKKERSEHLLDSLRKNLLSMPIAVSDKREDGSTYQVFEGNRRITALRMLSGEPALAAEWKGSLSKKFSEIDDLEAYKCEVLPCADRETADTILARRHTGGLQGIGPVTWDAKQKARFIRGENGQSSRLIALIDSFVNDPQAIRRIADVESLSDLMVRPDIRLFLGLDDDELERMDPGLSRGKRERLFTIIAKARSGDLPSANGESVAGYLNRIVSDRFKTPYKPDPAMRALAAVAYKQYLGASKAAEQASNHSSNPGPNSGPSGPGSGSKTNQNTHITSNDRGNAKNPPGRSGSGVASKDDLPALKKPVHKAIRRIHSYLHQDGHFPLDTNIRLFLRSAELTDQRMYAGMRNADLAPHAAVLEKLGESIVALRS",
    "response#3": "MIIIATKNKDKIKEIEEILSNSKINLIGLKDFPEIPEIVEDGETFEENAYKKALILSQKFGMPSLGDDSGLVVEALNGKPGVFSARYAGEHGNDKKNNEKLLLEMKEVPYSKRDAKFRCVIALALPNGEKKVVEGVCEGVITNTPRGDKGFGYDPIFYLPKLGKTMAELTFEEKNKISHRGKALRKMKEILKTL"
  },
  {
    "reference": "MQVQPTKYVGLVADLMPNIRLMQASGHFLFRYVTGPILIRKVYSWWTLAMVLIQFFAILGNLATNADDVNELTANTITTLFFTHSVTKFIYFAVNSENFYRTLAIWNQTNTHPLFAESDARYHSIALAKMRKLLVLVMATTVLSVVAWVTITFFGESVKTVLDKATNETYTVDIPRLPIKSWYPWNAMSGPAYIFSFIYQIYFLLFSMVQSNLADVMFCSWLLLACEQLQHLKGIMRSLMELSASLDTYRPNSSQLFRAISAGSKSELIINEEKDPDVKDFDLSGIYSSKADWGAQFRAPSTLQTFDENGRNGNPNGLTRKQEMMVRSAIKYWVERHKHVVRLVSAIGDTYGPALLLHMLTSTIKLTLLAYQATKIDGVNVYGLTVIGYLCYALAQVFLFCIFGNRLIEESSSVMEAAYSCHWYDGSEEAKTFVQIVCQQCQKAMTISGAKFFTVSLDLFASVLGAVVTYFMVLVQLK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The Extracellular, Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.2. The protein should contain a Helical; Name=3, Helical; Name=7, Helical; Name=5, Helical; Name=1, Helical; Name=6, Helical; Name=2 membrane-spanning region.3. The protein must exhibit the following characteristics: Odorant coreceptor which complexes with conventional odorant receptors (ORs) to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability; Plays a key role in preferred attraction of females for humans over non-human hosts for blood feeding; Human attraction plays a crucial role in the transmission of Plasmodium protozoans by the mosquito leading to infection diseases like malaria; Orco is a universal and integral part of the functional odorant receptor, involved in the dendritic localization of other olfactory receptors; Can form functional ion channels in the absence of an odor-binding OR; Also required for the response to N,N-Diethyl-meta-toluamide (DEET), the most widely used insect repellent worldwide..4. A protein that contributes to behavior, sensory perception of smell, signal transduction, detection of chemical stimulus involved in sensory perception of smell, sensory perception of taste by functioning in membrane with taste receptor activity, olfactory receptor activity, odorant binding. The designed protein sequence is ",
    "response#1": "MAYPFSTETFLFGKIPEHLYRILSSQSHHAETLLDSLLEQYDDYKTTYDSLKEQLNKTETELLEMQEKYNGLKSHISSGQHDTKDCPSSSVQHQPEAQSPSPSPQSLPPSPAPSPAPTPSPAPSSTASPAPSSSPAPSPAPSSSPAPSPAPSSSPAPSPAPSSSPAPSPAPSSSPAPSPAPSSSPAPSSSPAPSPAPSSSPAPSPAPSSSPAPSPAPSSSPAPSSSPAPSSSPAPSSSPAPSSSPAPSSSPAPSSSPAPSSSPAPSSSPAPSPAPSSSPAPSPAPSSSPAPSSSPAPSSSPAPSSSPAPSSSPAPSSSPAPSSAPSSAPSSAPSSAPSSAPSSAPSSAPSPASSPAPASSPAPSSSPAPSPAPSSSPAPSSSPAPSSSPAPSSSPSPASSPAPSSSPAPSSAPTSSPAPSSAPPSEPKSSSPVPSPSPSPAPSS",
    "response#2": "MMSFSLNPWWNSFALPLLLFCFLFHAHASEKLHKVVVIGGYRGSVLRDADGRLVWHVPLSSGMNVTSVCLCKSEGKFAYVTAGKSILELDENLKVTRRWSVGSGASCIALDHDNNIIYVGTDDAHILKSLDTCEELWRVKVNGSPTSLVLADDTLYVSTYDGSVFALSMEDGRTLSKRAWGPNSVPLSSPVVCGSLLYVGCSDAAFYAFDKESGEPKWKSKLGGSIMASPTSLSIEEGSIDIVVVGFDNAVFCFDRNGRLKWQQRLGGPIISTPAISENYVVVGCSDAHLYCFSRKEK",
    "response#3": "MDKILSNINNTDLSKNDIMGIYAWLKSVTPTQTKSLCAQIMSSQPQSTTSTSTTSSNFTKNENSNSNNTISNTNTNTSSNSSTSTNINNVNNNNSTSNNAISTTINNPSTSTTNNTNNSSTSNTNNTNATTTTNTATTNSTITPTSTTSTSSTSSTSNSSTSSTSSTSNTSNTSNTSNTTNTSNTSNSSNTSNTSNTSNTTNTSNTSNTSNTSNTSNTSNTSNTSNTSNTNTTNTTSTNSTTSNTSNTSTTNNSSNSTNSNSSTTNSNTNNTTSSSNNTISNSDSNTSNTSNTSSNNTTTNTTNTTN"
  },
  {
    "reference": "MQRVETPLYDAAQIRQGETQLFAHHDSFAIMQRAAEALLTVINECFPLAKHLTDNVDENAAENITENRAENRGTYRVVLGCGNNAGDGLVLAARLKQQGQAVICYTVFDQPRSQSLPPFTGDAAQAYQMAQDSGVPIVPVAKWQSQPTDVIIDAIFGIGLDRPPTGDAQWAIRAINQANAAAVVAVDVPSGLRCDTGQAYTDVADEAGDIDSIDSIEGADLNAVQADMTVTFIGDKIGLHTADGKGLSGQVVVATLGAVLPANLAEATVVRYHCVYHRVYHCGADERRRQQQLNQSGQSRQSGQSGQSRRFSSGNQHKGDFGHVLTIGGGQGMFGGAALAAVSALKVGAGKSSLYAHPDYQSQFHLDGTPLYEVMRCHDLHAIDASICFNAICLGPGLGRDDWGWAQFKTVTERLTTNQRLLIDADGLYHLANSDNHSANRLENAIAVITPHEGEAARLLQITVAAVRQDKPAIVKQLAQTYRCIAVLKGAGTLISDGNSVWINESGNVNLATAGTGDVLAGVISGLLAQGYTPLDAALQGVYRHGLAADDYCAEKKRHSVNKAGGKTLRASDLWSYF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.2. The protein must be able to ADP-dependent NAD(P)H-hydrate dehydratase activity. The designed protein sequence is ",
    "response#1": "WHEIAEISLPPLDPMGHKYDRGLIHCLSGAMPGAIALSAKAAARAGAGYVRISTSRMIDGLPSAIVQTEAADALDDERIGCLLVGPGMGDIPPLLTVALTKKHPLVIDADALLHVGEPERLQGHDAILTPHEGEFKNLFGDIEGSKADRALIAAQQTQCVVVYKGPDTLIASPDGRLGFAPPAPAWLASAGTGDVLAGIIAAHRARGMEPFEAACSAVYRHGRAAQRAGEGMIADDLVDAL",
    "response#2": "GVFSGGPSATGAARLAAMAAARIGAGAVTVLSPSLSLSVNASHLTSIMLRKADTLEEVFGFLDARHPEALVYGPGLGPEPGVGNFLLDLLAAAENSPKGLVLDADGLTSLAAQPEALFAALHRMGAPRAILTPHEGEFRRLFPGIAEDEKLSKLEKARAASRLSNAVVVYKGPDTVIAAPDGRAAINSNGTPLLATAGSGDVLAGICAGLLAQGMPAFEAGCAAVWIHADAAQRR",
    "response#3": "WHEIAEISLPPLDPMGHKYDRGLIHCLSGAMPGAIALSAKAAARAGAGYVRISTSRMIDGLPSAIVQTEAADALDDERIGCLLVGPGMGDIPPLLTVALTKKHPLVIDADALLHVGEPERLQGHDAILTPHEGEFKNLFGDIEGSKADRALIAAQQTQCVVVYKGPDTLIASPDGRLGFAPPAPAWLASAGTGDVLAGIIAAHRARGMEPFEAACSAVYRHGRAAQRAGEGMIADDLVDR"
  },
  {
    "reference": "MEPVDPRLEPWKHPGSQPGTPCNNCYCKKCCFHCQVCFTRKGLGISYGRKKRRQRRRPPQDSQTHQAPLSKQPSSQSRGDPTGPKKSKEKVERETEADQKD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein's functional design necessitates the inclusion of a Nuclear localization signal, RNA-binding (TAR), and protein transduction, Cell attachment site motif.2. The protein must exhibit the following characteristics: In the cytoplasm, Tat is thought to act as a translational activator of HIV-1 mRNAs.; Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to promote processive transcription elongation by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts; The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive; Tat can reactivate a latently infected cell by penetrating in it and transactivating its LTR promoter; Besides its effect on RNA Pol II processivity, Tat induces chromatin remodeling of proviral genes by recruiting the histone acetyltransferases (HATs) CREBBP, EP300 and PCAF to the chromatin; Through its interaction with host TBP, Tat may also modulate transcription initiation.3. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "MEPVDPNLEPWNHPGSQPKTPCNKCYCKQCSYHCLVCFQKKGLGISYGRKKRRQRRSAPPSSEDHQNLISKQPLPRTQGDQTGSEEPKKKVESKTETDPFD",
    "response#2": "MDPVDPNLEPWNHPGSQPKTACNNCYCKRCSYHCQKCFLKKGLGISYGRKKRRQRRRTPQGSKDHQNPIPKQPLPTTRGNPTGPKESKKKVESKAETDRFD",
    "response#3": "MEPVDPNLEPWNHPGSQPTTACSKCYCKKCCWHCQLCFLKKGLGISYGRKKRRRRRGTPQSQKDHQNPVPKQPLPTTRGNPTGPEESKKKVASKTETDQFA"
  },
  {
    "reference": "MKIMKFIFITGGVISSLGKGITAASLGRLLKARGFKVNMIKIDPYLQIDAGTMSPYEHGEVFVTEDGGESDLDLGHYERFIDENLTKNNNITTGKIYWSVLTKERKGEYLGKTVQVIPHITNEIKDWIKNLGEGYDITIVEIGGTVGDIESLPFLEAIRQFKKDVGKENVLYIHVSLLPYIRAAGELKTKPTQHSVKELRSIGIQPDILICRTEMPISDKIREKLALFCDVDKEAVIEARDARTIYEVPLNLEKEGLGKLVTKKLNLPDREPDLDEWRKFVDRVINPLNEVTIGIVGKYVELKDAYLSITEALIHAGAKNDTKVNINWIHSERLESEEFEELLDRYREDNQLDGILVPGGFGDRGVEGKINAIKYARENDIPFLGICMGMQCAVIEFARNVCGLEGANSTEFDENTKYPVVDLLPEQKEIDAKGGTMRLGAYPAILMEGTLAYKLYGRKEVYERHRHRYEVNPEYHEILENHGLTISGKSPDGRLAEFIEISKNRYFIATQAHPEFKSRPNKPHPLFDGLVRASLGEKIK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have at least one Glutamine amidotransferase type-1 domain.2. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates..3. A protein that is capable of catalyzing reactions within the Pyrimidine metabolism pathway with high efficiency and specificity.4. The designed protein is the optimization of the active site, Nucleophile; for glutamine hydrolysis to achieve high catalytic efficiency for ATP + NH4(+) + UTP = ADP + CTP + 2 H(+) + phosphate.5. The protein should be able to bind ATP, CTP, Mg(2+), L-glutamine, UTP ligand in a variety of conditions6. The designed protein should be involved in glutamine metabolic process, CTP biosynthetic process, 'de novo' CTP biosynthetic process by localizing to cytosol and exhibiting metal ion binding, identical protein binding, glutaminase activity, ATP binding. The designed protein sequence is ",
    "response#1": "MPDAYLSLLESLTHAGIANDARVNIKWVNAEELAEGDLETQLGDADGILVPGGFGIRGIEGKIKAAEYARTHNVPYLGICLGMQIAVIEYARHVAGLTGANSAEFDPYAPHKVIDLMPEQLEVEGLGGTMRLGDWPMELRAGTKIAELYGVPQGGTVRERHRHRYEVNPAYVGQLQDAGLVISGVTPGVQGRGAGLVESIEIPGHPFFVALQAHPEFKSRPMRPSPPFAGFVDAYKSIYESLDHAGFSHNSRVLVKRIEAEELEEQDPEIVLSGIDGLLVPGGFGYRGIEGKVKAIQFARECEIPYFGICLGMQCAVIEFARNRLGLAEANSSEFSAETPDPVICLLEEQKTVTDKGGTMRLGTQPCQLVENSRAAACYGTSEVSERHRHRYEFNPEYEQAFEKAGMIAAGKSPSGKLVEVVEIPAHPWFVAVQFHPEFKSKPHAAHPLFRGFVKDAYLSVVEALHHAGIHHDVKVNIRSVEAEEIEEHGSEKILSGAHGILVPGGFGSRGIEGKIAAARFARERKIPYLGLCLGMQVAVVEFARHVCSLPGAHSSEMDPGCANPVIHLMEEQMRVEDMGGTMRLGTYPCELVPGTKTASAYGVARVNERHRHRYEFNNDYRKRLEEKGMVIAGVYEEKNLVEIVELQDHPWFVGVQFHPEFRSRPVKPHPVFMGFLQAQA",
    "response#2": "MLDTYASLVEALKAACWQNGYDLELHWIDAEKIEKKDKEAWDALKNAKGVVVPGGFGVRGTEGKIDAIEFARKNNIPYLGLCLGMQLAAIEFARNVAGVNQATSEEFDEDSKACVIHIMDDQKTITEKGGTMRLGGWDCVLDPKSRIAKAYGTKKIRERHRHRYEFNNDFREKLESKGMMIAGTTPDGRLVEVVEIPSHKWFVAVQFHPEFTSRPLTGHPLFNAFIEAIA",
    "response#3": "MPDAYLSLLESLTHAGIANDARVNIKWVNAEELAEGDLETQLGDADGILVPGGFGIRGIEGKIKAAEYARTHNVPYLGICLGMQIAVIEYARHVAGLTGANSAEFDPYAPHKVIDLMPEQLEVEGLGGTMRLGDWPMELRAGTKIAELYGVPQGGTVRERHRHRYEVNPAYVGQLQDAGLVISGVTPGVQGRGAGLVESIEIPGHPFFVALQAHPEFKSRPMRPSPPFAGFVEAYRAVAEREGRLPDAYLSVVEAVRAAGYANWTRTNIKWITSDDCETPSGAMKALSGLDAIVVPGGFGIRGIEGKIGAITFAREHKIPLLGLCLGLQCTVIEAARQAGLEQASSTEFDPAATQPVIATMEEQKAAVSGEADLGGTMRLGAYPATLEEGSLVAELYGTTEVSERHRHRYEVNNAYRAQIAEGSDLVFSGTSPDGHLVEFVEYPKEVHPYLVATQAHPEYKSRPTHAHPLFYGLVDAYKSIYESLDHAGFSHNSRVLVKRIEAEELEEQDPEIVLSGIDGLLVPGGFGYRGIEGKVKAIQFARECEIPYFGICLGMQCAVIEFARNRLGLAEANSSEFSAETPDPVICLLEEQKTVTDKGGTMRLGTQPCQLVENSRAAACYGTSEVSERHRHRYEFNPEYEQAFEKAGMIAAGKSPSGKLVEVVEIPAHPWFVAVQFHPEFKSKPHAAHPLFRGFVKAAEEALAGKDAYLSVVEALHHAGIHHDVKVNIRSVEAEEIEEHGSEKILSGAHGILVPGGFGSRGIEGKIAAARFARERKIPYLGLCLGMQVAVVEFARHVCSLPGAHSSEMDPGCANPVIHLMEEQMRVEDMGGTMRLGTYPCELVPGTKTASAYGVARVNERHRHRYEFNNDYRKRLEEKGMVIAGVYEEKNLVEIVELQDHPWFVGVQFHPEFRSRPVKPHPVFMGF"
  },
  {
    "reference": "MAFALRFFTCFVLTVFIVASVDAAITCGTVASSLSPCLGYLSKGGVVPPPCCAGVKKLNGMAQTTPDRQQACRCLQSAAKGVNPSLASGLPGKCGVSIPYPISTSTNCATIK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design must be focused on enhancing the efficiency of response to abscisic acid, response to water deprivation, response to salt stress.2. For general function, the protein need meet that Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes; May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues .3. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "MSPSSGPSGAPDCMANLMNMTGCLSYVTVGEGGGAAKPDKTCCPALAGLVESSPQCLCYLLSGDMAAQLGIKIDKAKALKLPGVCGVITPDPSLF",
    "response#2": "MSPSSGPSGAPDCMANLMNMTGCLSYVTVGEGGGAAKPDKTCCPALAGLVESSPQCLCYLLSGDMAAQLGIKIDKAKALKLPGVCGVITPDPSLF",
    "response#3": "MSPSSGPSGAPDCMANLMNMTGCLSYVTVGEGGGAAKPDKTCCPALAGLVESSPQCLCYLLSGDMAAQLGIKIDKAKALKLPGVCGVITPDPSLF"
  },
  {
    "reference": "MKVALGVSGGIAAYKAAEVVRLLQERGIRVQVIMTRAAQEFIRPLTFAALSGEKVITDMFAPGDEQPNIDSAVEHIAVAQSIDALAVVPATADVLGKFAQGLATDFLTTLYLATTAPVVVAPAMKVNMWEHAATQANLEILRKRGVKIVEPGSGYLACGMTGPGRLAENEAIVAAVMEALGASQDLAGETVLITAGPTREKIDPVRYITNRSSGRMGYALAEAALRRGARVLLVSGPSALTPPGAAETTRVESAEEMRAAVLKFLPESSVVIKTAAVSDYKPKSMAPQKIKRSGPVALELEPTSDILAEIGRAKEAHGSASGERPKIIVGFAAETENILENARKKLSAKSADAIVVNDVSREGVGFDSERNAVTIITQDEVVEVPETTKWEVAQRVLDQVVRLRRTRSVPIKAGR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that can modulate the overall flux of metabolites through the coenzyme A biosynthesis pathway, enhancing or suppressing its activity as needed.2. The designed protein should be optimized for metal ion binding, phosphopantothenate--cysteine ligase activity activity in order to improve coenzyme A biosynthetic process. The designed protein sequence is ",
    "response#1": "KRILLGVSGGIAAYKSVTLASRLTQANALVDVIMTESATKFIAPVTFQAITQRPVSTDIFDSWSDTGRLRIGHVALAHAADLFIIAPATAQTLARLALGMADDLLSITTLSYKGPMLIAPAMETNMWHHPATQEHVAKLRGWGVDFVGPAEGRLASGEYGAGRVVEPEEVFAACRAVVAE",
    "response#2": "KNXLLGVTAGIAAYKSAFLVRLFVKKGANVKVVMTPSSKEFVTPLTLSTLSKNEVYSSFTKEDNENAQWNNHVELALWADLMLIAPATANTLSKMANGTCDNLLLAVYLSXKXPVYXAPAMDLDMYKHPSTLXYFKKLNEFGNIQIPADFGELASGLEGQGRMAAPENIISLVEQQF",
    "response#3": "KNXLLGVTAGIAAYKSAFLVRLFVKKGANVKVVMTPSSKEFVTPLTLSTLSKNEVYSSFTKEDNENAQWNNHVELALWADLMLIAPATANTLSKMANGTCDNLLLAVYLSXKXPVYXAPAMDLDMYKHPSTLXYFKKLNEFGNIQIPADFGELASGLEGQGRMAAPENIISLIEAHLA"
  },
  {
    "reference": "MGSCASCANKDKCSSASKDGGCSSSVPAKLGTNYGNIKNVIGVISGKGGVGKSTVTGILATQLAKKGYKVGVLDADITGPSMPRFFGINEKRADIVAMDSEGKQVKFVPVKTELGIKVISMNLLMEVEDDPVIWRGPMVTGVLNQMFKDTDWEELDYLLIDMPPGTSDITLTVMQTFPIKELVIVSTPQDMVSMIVKKLVTMAHKMNVCVRGVVENMAYIECECGKKMRVFSKKSSEEHAEYLGLPLIGELPINLDLTEALENGKAEEYVAENPLYSLIFEGLY",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Can hydrolyze ATP..2. The protein must have high specificity towards iron-sulfur cluster binding, metal ion binding, ATP hydrolysis activity, ATP-dependent FeS chaperone activity.3. The protein should have a high affinity for ATP. The designed protein sequence is ",
    "response#1": "HKRAAGIDLGTTNSLVASVRSGTADTLKDAQGRSLLPSIVNYANEEAIVGYEAKALSESQPQDTIISVKRLLGRSLTDIQTRYPSLPYRFKASENGLPVLQTTQGDKNPIEVSADILKVLAKRAEESLGGELSGVVITVPAYFDDAQRAGTKDAAKLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISILRLSKGVFEVLATGGDSALGGDDFDHLLADFLAEQAGLETPLSAEKNRTLLNIATATKIAFSEQDSVEVEVFGWKGVVTREQFEELIRPLVKKTLMSCRRALKDADVEADEVLEVVMVGGSTRTLLVREMVGEFFGRTPLTNINPDEVVAIGAGIQADILAG",
    "response#2": "RRLAAGIDLGTTHSLVAVVRSGQTETLADSENRHLLPSVVQYRKEDIQVGWQARQQAASDPVNTVSSVKRMMGRSLADIQKRYPNLPYQFQASENGLPLINTAAGLVDPIQVSSDILKSLAQRAEKTLDGKLDGVVITVPAYFDDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDVSILRLSRGVFEVLATGGDTALGGDDFDLLLANWIREQAGISDNDHRLQRQLLDIATQTKIVLSEADSAEICFADWQGRITRNEFNELITSLVKRTLLSCRRALKDAGVTADEVLQVVMVGGSTRVPLVRNMVGEFFDREPLTSIDPDRVVAIGAAIQADILVG",
    "response#3": "HKRAAGIDLGTTNSLVASVRSGTADTLKDAQGRSLLPSIVNYANEEAIVGYEAKALSESQPQDTIISVKRLLGRSLTDIQTRYPSLPYRFKASENGLPVLQTTQGDKNPIEVSADILKVLAKRAEESLGGELSGVVITVPAYFDDAQRAGTKDAAKLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISILRLSKGVFEVLATGGDSALGGDDFDHLLADFLAEQAGLETPLSAEKNRTLLNIATATKIAFSEQDSVEVEVFGWKGVVTREQFEELIRPLVKKTLMSCRRALKDADVEADEVLEVVMVGGSTRTLLVREMVGEFFGRTPLTNINPDEVVAIGAGIQADILAG"
  },
  {
    "reference": "MSAPIIAIDAMGGDFGPHCVVPASIAFLAENSSSQLILVGQPALLEELLSRYPSLDRQRLRVHAASEVIGSDERPSQALRGKPDASMRVALELVRDGRAHACVSAGNTGALMALSRHLLKTLPGIDRPAMVTAVPTEKGHCHLLDLGANVDCSAEHLYQFAVMGAVAAEALGCVSPRVALLNVGTEEIKGNQQVKLAASLLQKAQGLNFSGYIEGDGLYRGEADVVVCDGFVGNILLKASEGLAAMVSARIEQRFRDGLAAKLVGALALPLLRRLRGDIAPARYNGASFLGLQGIVVKSHGSAAEEGFQSALRRAALEVRENLPQRLHGRLEHLLL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that can perform phosphate:acyl-[acyl carrier protein] acyltransferase activity in cytoplasm to support fatty acid biosynthetic process, phospholipid biosynthetic process.2. The protein must exhibit the following characteristics: Catalyzes the reversible formation of acyl-phosphate (acyl- PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP).3. A protein that can modulate the overall flux of metabolites through the Lipid metabolism, phospholipid metabolism pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "EMILLG",
    "response#2": "MEEKLDLSKY",
    "response#3": "LCFRLG"
  },
  {
    "reference": "MGKKTFREWQYFKLSITSFDQDVDDAHAIDQMTWRQWLNNALKRSYGIFGEGVEYSFLHVDDKLAYIRVNHADKDTFSSSISTYISTDELVGSPLTVSILQESSSLRLLEVTDDDRLWLKKVMEEEEQDCKCI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be designed to localize specifically to ribonuclease MRP complex, nucleolar ribonuclease P complex in order to perform its role in nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay, intronic box C/D RNA processing more effectively.2. The design objective is to achieve a protein with strand-helix-helix-helix-strand-strand-helix-helix-strand-strand-helix characteristics.3. The protein must exhibit the following characteristics: Also a component of RNase MRP, which cleaves pre-rRNA sequences.; Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.4. The protein have enhanced Endonucleolytic cleavage of RNA, removing 5'-extranucleotides   from tRNA precursor.. The designed protein sequence is ",
    "response#1": "MLEFDTIKDSKQLNGLALAYIGDAIFEVYVRHHLLKQGFTKPNDLHKKSSRIVSAKSQAEILFFLQNQSFFTEEEEAVLKRGRNAKSGTTPKNTDVQTYRYSTAFEALLGYLFLEKKEERLSQLVAEAIQFGTFE",
    "response#2": "MLEFDTIKDSKQLNGLALAYIGDAIFEVYVRHHLLKQGFTKPNDLHKKSSRIVSAKSQAEILFFLQNQSFFTEEEEAVLKRGRNAKSGTTPKNTDVQTYRYSTAFEALLGYLFLEKKEERLSQLVAEAIQFGTHFISSLDELVELNEKLLNCQEFAVDLEHHSYRSFLGLTCLMQISTRTEDFIIDTLELRSDMYILNESLTDPAIVKVFHGADSDIEWLQKDFGLYVVNMFDTHQAARLLNLGRHSLDHLLKLYCNVDSNKQYQLADWRIRPLPEEMLSYARDDTHYLLYIYDKMRLENTQQLTAFQLLFAWRDKTARREDESYGYVLPNHMMLKIAEELPKEPQGIIACCNPVPPLVRQQINEMHLLIQQAREMPLLERNTQQLTAFQLLFAWRDKTARREDESYGYVLPNHMMLKIAEELPKEPQGIIACCNPVPPLVRQQINEMHLLIQQAREMPLLERQLELRALGNANTQQLTAFQLLFAWRDKTARREDESYGYVLPNHMMLKIAEELPKEPQGIIACCNPVPPLVRQQINEMHLLIQQAREMPLLERQLELRALRNA",
    "response#3": "MLEFDTIKDSKQLNGLALAYIGDAIFEVYVRHHLLKQGFTKPNDLHKKSSRIVSAKSQAEILFFLQNQSFFTEEEEAVLKRGRNAKSGTTPKNTDVQTYRYSTAFEALLGYLFLEKKEERLSQLVAEAIQFGTL"
  },
  {
    "reference": "MAPHQKSRINRINSTRSVPLRLATGGVLATLLIGGVTAAATKKDIIVDVNGEQMSLVTMSGTVEGVLAQAGVELGDQDIVSPSLDSSISDEDTVTVRTAKQVALVVEGQIQNVTTTAVSVEDLLQEVGGITGADAVDADLSETIPESGLKVSVTKPKIISINDGGKVTYVSLAAQNVQEALELRDIELGAQDRINVPLDQQLKNNAAIQIDRVDNTEITETVSFDAEPTYVDDPEAPAGDETVVEEGAPGTKEVTRTVTTVNGQEESSTVINEVEITAAKPATISRGTKTVAANSVWDQLAQCESGGNWAINTGNGFSGGLQFHPQTWLAYGGGAFSGDASGASREQQISIAEKVQAAQGWGAWPACTASLGIR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Active in the pM concentration range; Has peptidoglycan (PG) hydrolytic activity.2. The protein can be expressed in extracellular region and perform hydrolase activity without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "FGPTNYYTTANGFAGFEYELAKQYADSLNVELRIVPTYSLDELFIKLNTGEVDLLASGLSITDKRLQRFRFAPSYETISQKLVFKQGNVRPRKVADLTGTLMVTSGSSYVENLEKLKQTNSELAWQESTEFDSEELLRKVLSGEIDYTIIDSNNLAINRRYYPEISIGFSINEPEPLAWMVSENSHDDILASLVEFFGTVHHDGTLLALDDKYYGS",
    "response#2": "FGPTNYYTTANGFAGFEYELAKQYADSLNVELRIVPTYSLDELFIKLNTGEVDLLASGLSITDKRLQRFRFAPSYETISQKLVFKQGNVRPRKVADLTGTLMVTSGSSYVENLEKLKQTNSELAWQESTEFDSEELLRKVLSGEIDYTIIDSNNLAINRRYYPEISIGFSINEPEPLAWMVSENSHDDILASLVEFFGTVHHDGTLLALDDKYYGS",
    "response#3": "FGPTNYYTTANGFAGFEYELAKQYADSLNVELRIVPTYSLDELFIKLNTGEVDLLASGLSITDKRLQRFRFAPSYETISQKLVFKQGNVRPRKVADLTGTLMVTSGSSYVENLEKLKQTNSELAWQESTEFDSEELLRKVLSGEIDYTIIDSNNLAINRRYYPEISIGFSINEPEPLAWMVSENSHDDILASLVEFFGTVHHDGTLLALDDKYYGS"
  },
  {
    "reference": "MAGWSCLVTGGGGFLGQRIICLLVEEKDLQEIRVLDKVFRPEVREEFSKLQSKIKLTLLEGDILDEQCLKGACQGTSVVIHTASVIDVRNAVPRETIMNVNVKGTQLLLEACVQASVPVFIHTSTIEVAGPNSYREIIQDGREEEHHESAWSSPYPYSKKLAEKAVLGANGWALKNGGTLYTCALRPMYIYGEGSPFLSAYMHGALNNNGILTNHCKFSRVNPVYVGNVAWAHILALRALRDPKKVPNIQGQFYYISDDTPHQSYDDLNYTLSKEWGFCLDSRMSLPISLQYWLAFLLEIVSFLLSPIYKYNPCFNRHLVTLSNSVFTFSYKKAQRDLGYEPLYTWEEAKQKTKEWIGSLVKQHKETLKTKIH",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. A protein should be able to manipulate the activity of enzymes in the Lipid metabolism, steroid biosynthesis pathway, either by activating or inhibiting their function.3. The protein must exhibit the following characteristics: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids.4. The designed protein should have 3-beta-hydroxy-delta5-steroid dehydrogenase activity, steroid delta-isomerase activity within cytoplasm to regulate C21-steroid hormone metabolic process, hippocampus development, steroid biosynthetic process. The designed protein sequence is ",
    "response#1": "LVTGAGGFVGQRIIKMLVQEKELQEVRALDKVFRPETKEEFSKLQTKTKVTVLEGDILDAQCLRRACQGISVVIHTAAVIDVTGVIPRQTILDVNLKGTQNLLEACVQASVPAFIFCSSVDVAGPNSYKKIVLNGHEEQNHESTWSDPYPYSKKMAEKAVLAANGSMLKNGGTLNTCALRPMYIYGERSPFIFNAIIRALKNKGILCVTGKFSIANPVYVENVAWAHILAARGLRDPKKSTSIQGQFYYISDDTPHQSYDDLNYTLSKEWGLRPNASWSLPCWDTAFLIQAVFEAGLHKDEKWKPMLMKSLQYLERQQIREDCVDQDVCYRQPRKGGWPFSNKDQGYGVSDCISEAMKAIILLQKVGGLPEVLEERRLFDAVDTLLLYQNSNGGMSSYEKRRGGEWLEMLNAAEVFGRIMIEYDYPECTTACVTALSLFNKYWPDYRTKEVKTLIRTAAEWIKSNQRPDGGWYGSWGICFTYAGMFALESMKHIGQTYATGENSRRGCDFLISKQRADGGWSESYKACETMEYVEHPSGSLVVQTAWALIGLMEADYPHVEPLKRGIQLIMDRQQPNGEWLQEAIEGVFNKSCMISYPNYKFTFTIKALGML",
    "response#2": "LVTGAGGFLGQRIVQMLVQEKELQEVRVLYRTFSPKHKEELSKLQTKAKVTVLRGDIVDAQFLRRACQGMSVIIHTAAALDIAGFLPRQTILDVNVKGTQLLLDACVEASVPAFIYSSSTGVAGPNSYKETILNDREEEHRESTWSNPYPYSKRMAEKAVLAANGSILKNGGTFHTCALRLPFIYGEESQIISTMVNRALKNNSIIKRHATFSIANPVYVGNAAWAHILAARGLRDPEKSQSIQGQFYYISDDTPHQSYDDLNYTLSKEWGFCLDSSWSLPCWDTAFLIQAVFEAGLHKDEKWKPMLMKSLQYLERQQIREDCVDQDVCYRQPRKGGWPFSNKDQGYGVSDCISEAMKAIILLQKVGGLPEVLEERRLFDAVDTLLLYQNSNGGMSSYEKRRGGEWLEMLNAAEVFGRIMIEYDYPECTTACVTALSLFNKYWPDYRTKEVKTLIRTAAEWIKSNQRPDGGWYGSWGICFTYAGMFALESMKHIGQTYATGENSRRGCDFLISKQRADGGWSESYKACETMEYVEHPSGSLVVQTAWALIGLMEADYPHVEPLKRGIQLIMDRQQPNGEWLQEAIEGVFNKSCMISYPNYKFTFTIKALGML",
    "response#3": "LVTGGCGFLGEHIVRMLLEREPRLRELRVFDLHLSSWLEELKAGPVQVTAIQGDVTQAHEVAAAMSGSHVVIHTAGLVDVFGKASPKTIHKVNVQGTQNVIDACVQTGTQYLVYTSSMEVVGPNIKGHPFYRGNEDTPYEAVHSHPYPCSKALAEQLVLEANGRKVNGGLPLVTCALRPTGIYGEGHQVMRDFYYQGLRFGGRLFRAVPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCGLRL"
  },
  {
    "reference": "MGAQVSTQKTGAHEASLSATGSSIIHYTNINYYKDSASNSANRQDFTQDPSKFTEPVKDVMIKSLPALNSPSAEECGFSDRVRSITIGNSTITTQECANVVVAYGRWPTYLRDDEATAEDQPTQPDVATCRFYTLESVQWQENSAGWWWKFPDALSNMGLFGQNMLYHYLGRAGYTIHVQCNASKFHQGCLLVVCVPEAEMGCSKVDGVVNAQGLSKGEIPIEFERTSTTAEVGVVQKAVYNAGMGIGVGNLTIFPHQWINLRTNNSATIVIPYINCVPMDNMFRHNNFTLMVIPFAPLKSSGGTNYVPVTITVAPMDAEYNGLRLAGHQGLPTMNTPGSTQFLTSDDFQSPCAMPEFDVTPCMDIPGKVHNLMEIAEVDIGVPVNNTSTHMEGTDAFQIKVTAGNVQDKNAIFSFQLNPGNSTVLRRTLLGEILNYYAHWSGSIKLTFLFCGSAMATGKLLLAYSPPGASVPKARRDAMLGTHVIWDVGLQSSCVLCVPWISQTHYRLVAQDEYTSAGYITCWYQTNIVVPPETPSDCVVLCFVSACNDFSVRMLKDTPFIEQTTELQSDVREAVEGAIGRVADTIRSGPSNSEAVPALTAAETGHTSQVVPSDTMQTRHVKNYHSRSESTIENFLCRSACVRMAKYEARGNLKALTLDAWEISVRDMVQLRRKCEMFTYLRFDVEVTFVITSYQRQGTSSIQICPYDAHQIMYIPPGGPIPKKVDGYEWQTSTNPSIFWTEGNAPPRMSIPFISIGNAYSSFYDGW",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have a binding site that is specific to the target Zn(2+) ligand. The designed protein sequence is ",
    "response#1": "MLAYATREAVARTATTGRAHYYSRSRDELWEKGATSGNTQSVDEIRVDCDGDALLYLVAQTGGACHTGHESCFL",
    "response#2": "MLAYATREAVARTATTGRAHYYSRSRDELWEKGATSGNTQSVDEIRVDCDGDALLYLVAQTGGACHTGHESCFM",
    "response#3": "MLAYATREAVARTATTGRAHYYSRSRDELWEKGATSGNTQSVDEIRVDCDGDALLYLVAQTGGACHTGHESCFL"
  },
  {
    "reference": "MRVKEKYQHLWRWGWRWGTMLLGMLMICSATEKLWVTVYYGVPVWKEANTTLFCASDAKAHETEAHNVWATHACVPTDPSPQEVRLENVTENFNMWKNNMVEQMQEDIINLWDQSLKPCVKLTPLCVTLNCINANTTNSSIWEGMKEEIKNCSFNITTNIKKKLKREYALFNKLDVVPLGDSNTSYMLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILQCNDNKFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAKEEVIIRSENFTNNAKNIIVQLNESVQINCTRPNNNTRKSIPIGPGGAFYATGDIIGDIRQAHCNISEADWNNTLKKIVIKLAEQINKTIVFNQSSGGDPEIVMHSFNCGGEFFYCNTTELFNSTWQSDGTRNDTNKKSNNGTIILPCRIRQIVNRWQEVGKAMYAPPISGQINCSSNITGLLLTRDGGPGNSTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGTLGAMFLGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNTSWSNKSYNNIWDNMTWMEWDREINNYTNLIYSLIEESQNQQEKNEQDLLALDEWASLWNWFDITKWLYYIKIFIMIVGGLIGLKIVFTVLSVVNKVRQGYSPLSLQTHLPVPQGPDRPEGTEEESGNRGRDRSGRLVIGSLALIWDDLRSLCLFSYHRLRDLLL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have at least one Retroviral envelope protein GP41-like domain.2. For general function, the protein need meet that The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes3. The protein should be optimized for stability and solubility when expressed in virion membrane, host cell plasma membrane, host cell endosome membrane, viral envelope where it will be involved in positive regulation of establishment of T cell polarity, clathrin-dependent endocytosis of virus by host cell, virion attachment to host cell.4. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells. The designed protein sequence is ",
    "response#1": "MRVRGMQRNWQHLGIWGILILGIIIICSASNNLWVTVYYGVPVWRDADTTLFCASDAKAHETEVHNVWATHACVPTDPNPQEILLENVTENFNMWKNNMVDQMQEDITSLWDQSLKPCVKLTPLCVTLNCTNWQKNGTNNNGSVNGTNNNESVIEAGEIKNCSFNVTTDLRDKKQKVSALFYKLDVVPINGNGTEYMLINCNTSVIKQACPKISFDPIPIHYCTPAGYTILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEMIIRSENLTNNAKTIIVHLNEPVEIKCTRPGNNTRASVHIGPGQVFYKTGDIIGDIRKASCEINGTKWDETLKQVAKKLREHFKNRTIKFQPPSGGDLEITLHHFNCRGEFFYCNTTKLFNNTWKDGNWTEGSNSINESITLPCRIKQIVNMWQGAGQAMYAPPIRGNISCVSSITGILLTRDGGTDNDTETFRPGGGNIKDNWRSELYKYKVVQIEPLGIAPTRARRRVVQREKRAVGIGALFLGFLGAAGSTMGAASLTLTVQARQLLTGIVQQQRNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTSVPWNSSWSNKTHDDIWNNLTWMEWEREIENYTREIYTLIEESQNQQEKNELELLELDKWASLWNWFSITKWLWYIKIFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTLTPSPRGPDRPEGIEEEGGERDRGG",
    "response#2": "MRVMGTQRNYPPFWRWGMIIFWIMMICNANNLWVTVYYGVPVWXDADTTLFCASDAKAYDKEVHNVWATHACVPTDPNPQEVNLTNVTENFNMWKNNMVEQMQEDIISLWDQSLKPCVKLTPLCVTLNCTKVNSNSTGWGKEEIKNCSFNITTELRDKRQKVYSLFYRLDVVPIEEGQGNSSNSGYKEYRLINCNTSAITQACPKVTFNPIPIHYCTPAGYAILKCNEETFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVIIRSENITNNVKNIIVQLAEPVRINCTRPNNNTRTSIRIGPGQTFYTSNIIGDIRQAHCNVSRAEWNKALNKIGKQLRKYFVNKTIKFANSSGGDLEITTHSFNCEGEFFYCNTSGLFNGTWNASMQESNSTESNETIILPCRIKQIINMWQRVGQAMYAPPIQGIIKCVSNITGLILTRDGKSSNSTDEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRAVGREKRAVGIGAMILGFLGAAGSTMGAASITLTVQARQLLSGIVQQQXNLLRAIEAQQHMLRLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTSVPWNSSWSNKTQKEIWENMTWLQWDKEISNYTDIIYSLIEESQNQQEKNEQDLLALDKWSTLWSWFDISNWLWYIXIFIMIVGGLIGLRIVFAVLTIINRVRQGYSPLSFQTLT",
    "response#3": "MRVKGIRKNYQHLWRWGTMLLGMLMICSAAEQLWVTVYYGVPVWKDADTTLFCASDAKAYETEAHNVWATHACVPTDPNPQEIPLENVTEQFNMWKNNMVEQMHTDIINLWDQSLKPCVKLTPLCVTLNCTKPNNTLWVTVYYGVPVWRDAETTLFCASDAKAHETEVHNVWATHACVPTDPNPQEITLVNVTENFNMWKNNMVEQMQEDVISLWDQGLKPCVKLTPLCVTLNCSHNITGINGTNPDNVVMNITEEARNCSFNVTTELQDRKKQVYALFYKLDTVQVGESNDTYMLINCNTSVIKQACPKMSFDPIPIHYCAPAGFAILKCNEKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEGEIIIRSENITDNTKNIIVHLNESVEISCTRPSNNTRQSTAMGPGRVFYRAGDITGDIRQAYCEINGTNWHTTLEKVAEKLKEHFNNTIISFQPPSGGDLEITMHHFNCRGEFFYCNTSKLFNKTYPGNETRENNITLPCRIKQIINMWQGAGQAMYAPPISGRINCVSNITGILLTRDGGNKTNETKEIFRPGGGNIKDNWRSELYKYKVVQIEPLGIAPTRARRRVVQREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNASWSNKSQKEIWENMTWLQWDKEISNYTDIIYHLIKESQNQQEKNEQDLLALDKWADLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSVINRVRQGYSPLSFR"
  },
  {
    "reference": "MGDDELAEIRRQRMMQMQQQQMAEQDQIQRQQQMQAQIQSVLMQVMEPEARERLNTIRLTKPEFAASVEQQIVSLAQSGRLRQKITDEQLRQLLTQIVPQKKEFNIRRVG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must be able to DNA binding. The designed protein sequence is ",
    "response#1": "LGNKINDEITKIAETQETIWGFVGKTMDLIEARTTRPTTKAAYNLLLQATFMNCCRADDLKNTDIKTFEVIPDKHLGRMLRAFVPETKTGTRFVYFFPCKGRCDPLLALDSYLQWTDPIPKTRTTDEDARYDYQLLRNSLLGSYDGFISKQSDESIFKIPNGPKAHLGRHVTASYLSNNEMDKEATLYGNWSAAREEGVSRVAKARYMHTIEKSPPSYLFAFLSGFYNITAERACELVDPNSNPCEQDKNIPMISDIETLMARYGKNAEIIPMDVLVFLSSYARFR",
    "response#2": "LGNKINDEITKIAETQETIWGFVGKTMDLIEARTTRPTTKAAYNLLLQATFMNCCRADDLKNTDIKTFEVIPDKHLGRMLRAFVPETKTGTRFVYFFPCKGRCDPLLALDSYLQWTDPIPKTRTTDEDARYDYQLLRNSLLGSYDGFISKQSDESIFKIPNGPKAHLGRHVTASYLSNNEMDKEATLYGNWSAAREEGVSRVAKARYMHTIEKSPPSYLFAFLSGFYNITAERACELVDPNSNPCEQDKNIPMISDIETLMARYGKNAEIIPMDVLVFLSSYARFI",
    "response#3": "LGNKINDEITKIAETQETIWGFVGKTMDLIEARTTRPTTKAAYNLLLQATFMNCCRADDLKNTDIKTFEVIPDKHLGRMLRAFVPETKTGTRFVYFFPCKGRCDPLLALDSYLQWTDPIPKTRTTDEDARYDYQLLRNSLLGSYDGFISKQSDESIFKIPNGPKAHLGRHVTASYLSNNEMDKEATLYGNWSAAREEGVSRVAKARYMHTIEKSPPSYLFAFLSGFYNITAERACELVDPNSNPCEQDKNIPMISDIETLMARYGKNAEIIPMDVLVFLSSYARFL"
  },
  {
    "reference": "MSMFCFQCQEAARNKGCTVRGVCGKTGDVANLQDLLVYLLKGISIYAEKAKELGVVDRETGAFITRALFTTITNVNFDNKRFEEIIKEAFEVRDRIKNKFLKAYREKNGEEFDEKLPGMATWYSNDVSDFYAKGEEVGVLSTGDEDVRALRELLTYGLKGIAAYAEHAYILEEEDQEIYSFLQEGLVATTDNTLSADELTALVMKCGEVAVKTMALLDQANTSNYGHPEPTQVNLGVRNRPGILVSGHDLKDLEELLEQTEGKGVDVYTHGEMLPANAYPAFKKYDHFVGNYGNAWWQQKEEFEKFNGPILMTTNCLVPPKDSYRDRVYTTNVVGFSGVKHIRDRKPGEQKDFSPVIEHALKCDPPEELENGMIPVGFAHNAVMSVADKVVEAVKQGKIKRFVVMAGCDGRHRSRDYYTNVARNLPEDAVILTAGCAKYRYNKLNLGDIDGIPRILDAGQCNDSYSLVVIAQKLAEVFGVDDINELPISYDIAWYEQKAVAVLLALLYLGVKGIRLGPSLPAFLSPNVVKVLVDKFDIKPIGEVEEDVKAIMAGE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining metal ion binding, 4 iron, 4 sulfur cluster binding, hydroxylamine reductase activity.2. The protein must exhibit the following characteristics: Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O..3. The presence of hybrid [4Fe-2O-2S] cluster is crucial for the protein to function optimally under specific conditions.4. The protein should be able to bind [4Fe-4S] cluster, hybrid [4Fe-2O-2S] cluster ligand in a variety of conditions5. The protein have enhanced A + H2O + NH4(+) = AH2 + H(+) + hydroxylamine. The designed protein sequence is ",
    "response#1": "MATAETFYDVIRRQGITRRSFTKFCSLTAASLGFGPGAATAMAEAKKLSEALLPKTRAYHEIWLDEEKVAGTPQQDEVEPMYGPLYLPRKFKIGVAVPPANDVDIFSQDIGFIAILEEGKLTGFNVACGGGMGMTHGDTKTYPQLGRIIGFCEPQQIMDVAEKLITIQRDYGNRSVRKYARFKYTIDRRGLDWLKNELHARLGWHYSQRIHDKCYRRPHFDAGQFVEEWDDEGARKGYCLYKMGCKGPTTYNACSTVRWNGGVSFPIQSGHGCIGCSEDGFWDNGSIKWDKAPCRFCGTGCSVLVGSQNGKVVATQGDPDAEVNKGLNCIKGYFLSKIMYGKPYSQRIHDKCYRRPHFDAGQFVEEWDDEGARKGYCLYKMGCKGPTTYNACSTVRWNGGVSFPIQSGHGCIGCSEDGFWDNGSYSQRIHDKCYRRPHFDAGQFVEEWDDEGARKGYCLYKMGCKGPTTYNACSTVRWNGGVSFPIQSGHGCIGCSEDGFWDNGSRFYSQRIHDKCYRRPHFDAGQFVEEWDDEGARKGYCLYKMGCKGPTTYNACSTVRWNGGVSFPIQSGHGCIGCSEDGFWDNGSRF",
    "response#2": "RLTQPLLRMKDGQYHKEGNFHPVSWDMAFDVMADKFKSALKAKGPTSVGMFGSGQWTVMEGYAAVKLMKAGFRSNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDLEQADTFVLWGSNMAEMHPILWSRLTDRRLGHAHVRVHVLSTYKHRSFALADNGMIFTPNTDLAIANYIANYIIQNDAVDWDFVKKHTHFKQVTTDIGYGLRPEHELQKAAKNVDSGKINNLTFEEYKNQVAEYTLEKTVEISGVPANKLEEMAKMYADPKKKVMSLWTMGMNQHTRGVWMNSLVYNIHLLTGKISKPGNGPFSLTGQPSACGTAREVGTFSHRLPADMVVKKKKHRDYAEKIWKLPEGTIPAKPGYHAALQNRMLKDGKLNAYWVMCNNNVQAAANLNEETLPGYRNPENFIVVSDPYPTVTCQAGDLILPTAMWVEKEGCYGNAERRTQHWFQQVRAPGEAKSDLWQLIEFSKRFKVEEVWPAELLAKKPQYRGKTLYDVLYRNGQVDKFPLSEVSAGYANDEAKKYGFYVQKGLFEEYASFGRGHAHDLDDYDSYHKGRWHLTLFIQNGRIQDTDDYQLMTGLREIAKIHTGEFRLTGNQNVIISNITAAKKKAIQAIGYSQRIHDKCYRRPHFDAGQFVEEWDDEGARKGYCLYKMGCKGPTTYNACSTVRWNGGVSFPIQSGHGCIGCSEDGFWDNGSYSQRIHDKCYRRPHFDAGQFVEEWDDEGARKGYCLYKMGCKGPTTYNACSTVRWNGGVSFPIQSGHGCIGCSEDGFWDNGS",
    "response#3": "RLTQPLLRMKDGQYHKEGNFHPVSWDMAFDVMADKFKSALKAKGPTSVGMFGSGQWTVMEGYAAVKLMKAGFRSNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDLEQADTFVLWGSNMAEMHPILWSRLTDRRLGHAHVRVHVLSTYKHRSFALADNGMIFTPNTDLAIANYIANYIIQNDAVDWDFVKKHTHFKQVTTDIGYGLRPEHELQKAAKNVDSGKINNLTFEEYKNQVAEYTLEKTVEISGVPANKLEEMAKMYADPKKKVMSLWTMGMNQHTRGVWMNSLVYNIHLLTGKISKPGNGPFSLTGQPSACGTAREVGTFSHRLPADMVVKKKKHRDYAEKIWKLPEGTIPAKPGYHAALQNRMLKDGKLNAYWVMCNNNVQAAANLNEETLPGYRNPENFIVVSDPYPTVTCQAGDLILPTAMWVEKEGCYGNAERRTQHWFQQVRAPGEAKSDLWQLIEFSKRFKVEEVWPEELLAKAPELKGKTLFDVLYRNGQVDRFPLSEIAPEYANDESKAFGFYVQKGLFEEYAEFGRGHAHDLAPYDFYHAVRGLRWPVVDGKATLRRFKTADPYGRGKPQFDYYGHKGLRWLVAGEPTEDAPDEFDMWLCTGRVLEHWHSGSMTQRVPELYRAFPDALVFMHPDDAKKRNLRRGDEVMVEP"
  },
  {
    "reference": "MEKWRTVIGLEIHAELLTKTKIFCNCSAEFGGAPNTRCCPVCTGMPGTLPIINQQAVEYAVKAGYALNCGINKFSVFDRKNYFYPDLPKAYQISQLYRPIVGPGYVPIEVNGQKKNIRINHIHLEEDAGKLVHDAFNGISMADYNRCGIPLIEIVTEPDIASAEEAMAFVEQISLLLQYAGVCDCKMEQGSLRCDVNISLMRPDATEFGTRAEIKNINSIKSVGRAIKYEEKRQALLLEAGKKVVQETRRYNANRDKTTSMRTKENANDYRYFPEPDILQVNLTDAQLDEIKAKLPEMPSSRFKRYTEEYGISEIDAKTLIQTKIISDFFENALKRYNNPKSVAVFILGEFMRRVNLGEIDINNISFTPEEFAELVEMADTEKVSKNDAKTVFRAMVEEGGKPMDIAKSKGMIITVDTAKVEAGVDEILAANAAQVEQYKNGETKVFGFIMGQCTKALKGVATPKIIKEILESKLKAAAASVASDAEKKEDVKDNVIDTSKLTKYENADKYVPENDGKMLMIDTADVKKEFMLADAKANMGKEVEFSGCVHRIKNMGSIAFIVVRTSRDVIQTVYSADNCKDSIEGLREGFFVNVKGIVKENPKDFNGLEIELNSIKLISTNAAELPLKISQGRLNCTIEVNLDNRAASLRNPYERAIFKLQEGLVQGMHKFMQANNFTEIHSPKIVAQGAEGGANIFRLDYFGKSAFLNQSPQFYKQMAVAFFDRVYEIAPVYRAEKHATSRHINEYIGLDFEMGYIDSMYDVMKME",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be designed to catalyze the following reaction ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-   aspartyl-tRNA(Asp).2. The protein should have ATP binding, aspartate-tRNA ligase activity and be active in cytoplasm.3. The designed protein should have a binding site that is specific to the target L-aspartate ligand.4. The designed protein must have at least one Aminoacyl-transfer RNA synthetases class-II family profile domain. The designed protein sequence is ",
    "response#1": "VMGLEVHVELSTKTKIFCSCTTEFGGLPNSHTCPVCLGMPGSLPVFNKSVADKAIKAGLALHCQIDRNNRFDRKNYFYPDLPKDYQISQLYAPICQNGYLDIELADGTAKKITIREIHMEEDAGKLIHDNSGTLIDYNRCGVPLVEIVTNPDFSSPEEVGVFLERLQEILVYLDVSDCKMQEGSMRADVNLSVRPEGQEEYGTRTEMKNLNSLKAIQRAIAFESERQIKVIQEGGTIRQETRRWDDEAGESYAMRSKENAQDYRYFPDPDLLPVRISDAWLFEETAARSGNAKESANIIIGDILKLMKETATLPENITIDSEKLAAAVQLIADGKINRSVGKTVVEELYKNNIDPAAYVKENGLLIIEDSSKIEPVVLQVLESHPQSIEDYKNGKEKAFDFLIGQCMRLLKGSASPKAINAMLREKL",
    "response#2": "VMGLEVHVELSTKTKIFCSCTTEFGGLPNSHTCPVCLGMPGSLPVFNKSVADKAIKAGLALHCQIDRNNRFDRKNYFYPDLPKDYQISQLYAPICQNGYLDIELADGTAKKITIREIHMEEDAGKLIHDNSGTLIDYNRCGVPLVEIVTNPDFSSPEEVGVFLERLQEILVYLDVSDCKMQEGSMRADVNLSVRPEGQEEYGTRTEMKNLNSLKAIQRAIAFESERQIKVIQEGGTIRQETRRWDDEAGESYAMRSKENAQDYRYFPDPDLLPVRISDAWIEQVRLELPEMPDAKFIKRHKIVTVIREYLNALGFLEIETPVLTKSTPEGARDYLVPSRIHHGRFYALPQSPQLFKQLLMMAGFDRYYQIVRCFRDEDLRPNRQPEFTQLDMEMSFIDEEFIYRTIEELMARAASVAGVVLSVPFPRMSYKTAVDEYGTDRPDTRFAMTFEDLTKVISETSYSVFRTIIERGGVIKGICARGQAGNLSKNVLQNEYALNIVPSFGARGMTWMKFVGGQLQSNIAQFFTEREKESIVDCFNAMEGDVIMMIADESADVVNKALCSLRLHLAERLSLIKPGNFSPLWVTNFPLFEEKEGRLYSQHHPFTMPDQVDFDPQNLRMLLNLNSRAYDLVVNGEELGGGSIRIHDMDTQKKIFEALGLNEQEIREKFGFFLRALEQGAPPHGGIALGMDRMVAMLLNAPSIRDVIAFPKNRQNEYVGAVVMDGGASQPRRQLDAWQEWAKQRGAKGLAYILVGEDGELSGPVAKNITDDERAGIADHVGAKPGDCIFFAAGDTKLD",
    "response#3": "VMGLEVHVELSTKTKIFCSCTTEFGGLPNSHTCPVCLGMPGSLPVFNKSVADKAIKAGLALHCQIDRNNRFDRKNYFYPDLPKDYQISQLYAPICQNGYLDIELADGTAKKITIREIHMEEDAGKLIHDNSGTLIDYNRCGVPLVEIVTNPDFSSPEEVGVFLERLQEILVYLDVSDCKMQEGSMRADVNLSVRPEGQEEYGTRTEMKNLNSLKAIQRAIAFESERQIKVIQEGGTIRQETRRWDDEAGESYAMRSKENAQDYRYFPDPDLLPVRISDAWIDALITALPELPWLAQAKGGRVVALRVPGGGEMSRSEIDGYTEFVKIYGAKGLAWIKVNDASKGREGLQSPIVKNLHDAAIAEIIARSGARNGDLLFFGADKAKVVNDR"
  },
  {
    "reference": "MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAANTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFITAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVDTLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTVMANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEAEEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATNYRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSVVSPVERFEAPISQDEDELDTPPFFKNR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. The designed protein sequence is ",
    "response#1": "VIKVVGIGGGGVNAVNRMIEQGLKGVEFIAINTDAQALLMSDADVKLDVGRDSTRGLGAGADPEVGRKAAEDAKDEIEELLRGADMVFVTAGEGGGTGTGGAPVVASIARKLGALTVGVVTRPFSFEGKRRSNQAENGIAALRESCDTLIVIPNDRLLQMGDAAVSLMDAFRSADEVLLNGVQGITDLITTPGTALMGIGSARGEGRSLKAAEIAINSPLLEASMEGAQGVLMSIAGGSDLGLFEINEAASLVQDAAHPDANIIFGTVIDDSLGDEVRVTVIAAGFH",
    "response#2": "GLAMIGIGESDSEKRAKEAVSMALNSPLLDVDIDGATGALIHVMGPEDLTLEEAREVVATVSSRLDPNATIIWGATIDENLENTVRVLLVITGVQ",
    "response#3": "GTALMGIGSARGEGRSLKAAEIAINSPLLEASMEGAQGVLMSIAGGSDLGLFEINEAASLVQDAAHPDANIIFGTVIDDSLGDEVRVTVIAAGFR"
  },
  {
    "reference": "MEISALQKARLEYSPKLPPALSGAIDAIAVSFGDPTESIANQAELKQLFAKTYGKPIAAFGKGANPNVGKELRVGVILSGGQAPGGHNVITGLYDGLKKANPNSVLFGFLGGPSGLIDDEAVEFTDEFIDRYRNTGGFDIIGSGRTKIETDAQFAAVLETAKKRKLNAVVVIGGDDSNTNAALLAEYFQTQGTDIQVIGVPKTIDGDLKNEYIETSFGFDTACKTYSELIGNIGRDANSAKKYWHFIKLMGRSASHIALECALQTQPNIAIISEEVESKKKSLAQIVEEIADVVAKRAAKGNNFGIALIPEGLIEFVPEMKILIAELNDLLAHNAEYFASLRTFDDQAEFVSGKLTDHSAKVFGSLPRGIRAQLMADRDPHGNVQVSLIDTEKLLIEMVGERLDQMKAAGSFKGSFKALAHFFGYEGRCAFPSNFDADYTYSLGFTAFLLIANGLTGYLSSVRNLSKSADQWVAGGIPLTMMMNMEQRHGAMKPVIRKALVELDGAPFKTFASHRDTWAVETSFRFPGAIQYYGPGEVCDQPTITLVLEQQA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein should be able to manipulate the activity of enzymes in the D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 3/4 pathway, either by activating or inhibiting their function.2. The designed protein must be able to perform diphosphate-fructose-6-phosphate 1-phosphotransferase activity under specific conditions.3. The protein should be able to bind diphosphate, Mg(2+), substrate ligand in a variety of conditions4. The presence of Phosphofructokinase domains is critical for the interaction of the designed protein with its intended targets.5. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.6. For general function, the protein need meet that Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis The designed protein sequence is ",
    "response#1": "MIVAQSGGPSPVINNSLRGLVETARDLPEIGTIYAGWHGIEGVLKEELLNLSGQSPEEIALLRVTPAAGSVGTCRYKLKEHQNEDFDRIIEVFKAHNVGYFCYIGGNDSMDTANKVAQMATERGVDVVGIGVPKTIDNDVGDSEFKLIDHTPGYGSTARYWLSMVQMANEENRGSCPADPVLVLQAMGRKIGFIPAAARLADPQRKIPMQIYLAENPVSIEQIHTQINDQLKKDGRLIVVVSEGLSLGDIGETKDSFGHTQFSSSQMTVAQLLVNELNQRGLAVKGAARANVPGTDQRRVGVLTGGGDCPGLNAVIRAIVRRGVPEYGYDFLGFRDGWRGPLEAETRPLDIPAVRGLLPRGGTILGSSRTNPFKIDDGVTRIRKNLQSLGVDALIAIGGEDTLGVASELHEKGVKVIGVPKTIDNDLSGTDYTFGFDTAVTIATEAIDRLHTTAESHHRALIVEVMGRHAGWIALHSGMAGGANAILIPEVRFDIDQVCSWVESRFRLDYAPIIVVSEGAMPQDGENVLVAGGKDEFGHARLGGIGDWLAHEIESRTGKEARTTVLGHVQRGGTPSARDRWLATRFGLHAIDLVHEGAFGMMAALRGEQIAVPFGELLGQPRRLRPEVVADLRHLARVLGPELLP",
    "response#2": "MIVAQSGGPSPVINNSLRGLVETARDLPEIGTIYAGWHGIEGVLKEELLNLSGQSPEEIALLRVTPAAGSVGTCRYKLKEHQNEDFDRIIEVFKAHNVGYFCYIGGNDSMDTANKVAQMATERGVDVVGIGVPKTIDNDVGDSEFKLIDHTPGYGSTARYWLSMVQMANEENRGSCPADPVLVLQAMGRKIGFIPAAARLADPQRKIPMQIYLAENPVSIEQIHTQINDQLKKDGRLIVVVSEGLSLGDIGETKDSFGHTQFSSSQMTVAQLLVNELNQRGLAVKGAARANVPGTDQRMIVAQSGGPSAVINNTVRGIVETARQMDEIGTVYAGWHGIEGVLKEELLDLSKQSAEEIALLRTTPAAGSFGTCRYKLKPGQDEDFDRVMEVFKAHDIGYFCYVGGNDSMHTAYTVANLARERGLDVCGLGGPKTIDNDVGDSEFKLVDHTPGYGSTARYWAHYIQQANEENRGSCPADPVLVLQAMGRKIGYIPAAARLADPNREMPLQIYLAEREVTIDQIHENVNNLLKERGRAIVVVSEGLSITGLEIPEEFIVRDSFGHPTFSSSKITVGQMLTNALNDRGLAVKGAARCNVPGTHQRSAWLGEKAAQAQSGGVTSVINASACGVIETARKHPDRIGNVYAGRNGIIGALTEELIDTGAEPAEAIAALRSTPAGAFGSCRYKLKSLEENRREYERLIEVFKAHGIGYFFYNGGGDSADTCFKVSQLSQSLGYPLQAIHVPKTIDNDLPLTDCCPGFGSVAKYVAVSTLEASFDVRSMAATSTKVFVLEVMGRHAGWIAAAGGLVADHGIPVVVLFPEIEFDKARFLARVDELVKQHGYCSVVVSEGCHYPDGTFLAEQGTRDAFGHAQLGGAAPVVAQMVKEALGHKFHWAVADYLQRAARHIASATDVKQAYELGQRAVE",
    "response#3": "MIVAQSGGPSPVINNSLRGLVETARDLPEIGTIYAGWHGIEGVLKEELLNLSGQSPEEIALLRVTPAAGSVGTCRYKLKEHQNEDFDRIIEVFKAHNVGYFCYIGGNDSMDTANKVAQMATERGVDVVGIGVPKTIDNDVGDSEFKLIDHTPGYGSTARYWLSMVQMANEENRGSCPADPVLVLQAMGRKIGFIPAAARLADPQRKIPMQIYLAENPVSIEQIHTQINDQLKKDGRLIVVVSEGLSLGDIGETKDSFGHTQFSSSQMTVAQLLVNELNQRGLAVKGAARANVPGTDQRRVGMLTGGGDCPGLNAVIRGAVRKGVTAHGHEFVGFRDGWRGPLEGDTMTLGIDEVRGILPRGGTILGSSRTNPFAIENGVERIKDNLAAAGCDALIAIGGEDTLGVATKLADLGVNVVGVPKTIDNDLSGTDFTFGFDTAVNIAMEAIDRLHTTAESHHRALVVEVMGRHAGWIALHSGLAGGANIILIPERPFDIDKVCAQVESRFATHYSPIIVVAEGATPLEGTMELVSGEKDAFGHVRLGGIGDRLAHEIEERTGREARAVVLGHIQRGGTPTAFDRWLATRFGLHAIQALIDGATGTMVSAQSGGVTSVINASACGVIETARKHPDRIGNVYAGRNGIIGALTEELIDTGAEPAEAIAALRSTPAGAFGSCRYKLKSLEENRREYERLIEVFKAHGIGYFFYNGGGDSADTCFKVSQLSQSLGYPLQAIHVPKTIDNDLPLTDCCPGFGSVAKYVAVSTLEASFDVRSMAATSTKVFVLEVMGRHAGWIAAAGGLVADHGIPVVVLFPEIEFDKARFLARVDELVKQHGYCSVVVSEGCHYPDGTFLAEQGTRDAFGHAQLGGAAPVVAQMVKEALGHKFHWAVADYLQRAARHIASATDVKQAYELGQRAVE"
  },
  {
    "reference": "MIIEPRMRGFICLTAHPTGCEQNIKNQIAYIKSKGHIEGAKRVLVIGASTGFGLASRITSAFGCDASTIGVFFEKPASQGKTASPGWYNSAAFENEAKKAGLYAKSINGDAFSNDVKQQTINLIKKDLGQIDLIIYSLASPVRLHPTTGVLHRSVLKPIGATFTNKTVDFHSGKVSEISIAPCEGDDIENTVTVMGGEDWSMWIDALKKENLLANNATTIAYSYIGPEVTEAVYRKGTIGKAKDHLEATAFSITDSLADLGGKAYVSVNKALVTQASSAIPVIPLYISLLYKIMKSEGIHEGCIEQMQRLYAQRLYTGNEIPTDDKGRIRIDDWEMRADVQEKVAKLWLEATTETLPEIGDLEGYRSDFHNLFGFGFEGVEYKADTNEMVNVSSII",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The NAD(+) binding site should be stable and able to withstand changes in environmental conditions.2. The protein should be designed to have high stability and activity towards a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] +   H(+) + NADH.3. The protein design must be able to perform enoyl-[acyl-carrier-protein] reductase activity, NAD binding in order to contribute to fatty acid biosynthetic process.4. The protein design should take into account the physiological conditions of the fatty acid biosynthesis, Lipid metabolism pathway.5. The protein must exhibit the following characteristics: Catalyzes the reduction of a carbon- carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP).. The designed protein sequence is ",
    "response#1": "KPGSPGWYNTVAFQNEAKKAGIYAKSINGDAFSTEVKQKTIDLIKADLGQVDLVIYSLASPVRTNPVTGVTHRSVLKPIGGAFSNKTVDFHTGNVSTVTIEPANEEDVTNTVAVMGGEDWGMWMDAMLEAGVLAEGATTVAYSYIGPALTEAVYRKGTIGRAKDHLEASAATITDKLKSVKGKAYVSVNKALVTQASSAIPVIPLYISLLYKVMKAEGIHEGCIEQIQRLYADRLKPGSPGWYNTVAFQNEAKKAGIYAKSINGDAFSTEVKQKTIDLIKADLGQVDLVIYSLASPVRTNPVTGVTHRSVLKPIGGAFSNKTVDFHTGNVSTVTIEPANEEDVTNTVAVMGGEDWGMWMDAMLEAGVLAEGATTVAYSYIGPALTEAVYRKGTIGRAKDHLEASAATITDKLKSVKGKAYVSVNKALVTQASSAIPVIPLYISLLYKVMKAEGIHEGCIEQIQRLYADRLKPGSPGWYNTVAFQNEAKKAGIYAKSINGDAFSTEVKQKTIDLIKADLGQVDLVIYSLASPVRTNPVTGVTHRSVLKPIGGAFSNKTVDFHTGNVSTVTIEPANEEDVTNTVAVMGGEDWGMWMDAMLEAGVLAEGATTVAYSYIGPALTEAVYRKGTIGRAKDHLEASAATITDKLKSVKGKAYVSVNKALVTQASSAIPVIPLYISLLYKVMKAEGIHEGCIEQIQRLYADRLLDEKGRLRLDDWELRDDVQGACKALWPQVTTENLFELTDYAGYKKQFLNLFGFERADVNYDEDF",
    "response#2": "KPGSPGWYNTVAFQNEAKKAGIYAKSINGDAFSTEVKQKTIDLIKADLGQVDLVIYSLASPVRTNPVTGVTHRSVLKPIGGAFSNKTVDFHTGNVSTVTIEPANEEDVTNTVAVMGGEDWGMWMDAMLEAGVLAEGATTVAYSYIGPALTEAVYRKGTIGRAKDHLEASAATITDKLKSVKGKAYVSVNKALVTQASSAIPVIPLYISLLYKVMKAEGIHEGCIEQIQRLYADRLTDDAQRIRMDDWELRESVQQACRDLWPQVTTENLSQLTDYQGYKAEFLKLFGFGWEGVDYTADHRTGTAGWYNNIFFKEFAEKEGLIAKNFIGDAFSDEVKENVIKYIKNEFGKIDLLIYSLASPRRKDPKTGNIYDSTLKTTSGEFQGPTIDMETDELVTTKVNSATDKEIEATKKVMGGEDWSEWCKLLLENDCLSDKAITISYSYIGASRTYKIYREGTIGEAKRHLENTAIQIDKEWQKKINGKAFVSVNKAIVTKASAYIPSFSLYAAVLYKVMKEKNLHENCIMQMQRMFADKIFDDSGRLRMDDWELREDVQSEVNELWEKITPDNFKILSDYDGYKKEFMQLNGFEIDGVNYSEDFVGSAGWYNNIFFKQEATHAGRTAINIVGDAFSDSVRNEVIEAIETYFEGEVDLVIYSLAAGVRPKPHSDTFWRSVIKPIGESVTGASILLENDQWVETTLEPATEEEAEATIKVMGGEDWESWIDTLINTESVAQGCKTIAFSYMGPEVTHPIYLDGTLGRAKIDLHQTSHALNLKLANFDGGAYATVCKALVTKASVFIPALSPYLLALYRVMKEKGTHERCIEQMQRLFTTKL",
    "response#3": "KAGTAGWYNAAAFDKFAKAEGLYSKSINGDAFSDEARAKVIELIKNEMGGQVDLVIYSLASPVRKLPQTGEVIRSALKPIGQPYKSTAIDTNKDTIIEASIEPATEQEIADTVTVMGGQDWELWIDALNAAGVLAPQARTVAFSYIGSDITWPIYWHGALGKAKQDLDETAQRLAGKVGGSANVAVLKSVVTQASSAIPVMPLYLSMVFKIMQEKGVHEGTQDQLDRMFRDRMFDDSGRLRMDDWELREDVQSEVNELWEKITPDNFKILSDYDGYKKEFMQLNGFEIDGVNYSEDDRTGTAGWYNNIFFKEFAEKEGLIAKNFIGDAFSDEVKENVIKYIKNEFGKIDLLIYSLASPRRKDPKTGNIYDSTLKTTSGEFQGPTIDMETDELVTTKVNSATDKEIEATKKVMGGEDWSEWCKLLLENDCLSDKAITISYSYIGASRTYKIYREGTIGEAKRHLENTAIQIDKEWQKKINGKAFVSVNKAIVTKASAYIPSFSLYAAVLYKVMKEKNLHENCIMQMQRMFADKITDEQGRIRIDDWEMREDVQANVAALWEQVTSENVSDISDLKGYKNDFLNLFGFAVNKVDYLADDVGSAGWYNNIFFKQEATHAGRTAINIVGDAFSDSVRNEVIEAIETYFEGEVDLVIYSLAAGVRPKPHSDTFWRSVIKPIGESVTGASILLENDQWVETTLEPATEEEAEATIKVMGGEDWESWIDTLINTESVAQGCKTIAFSYMGPEVTHPIYLDGTLGRAKIDLHQTSHALNLKLANFDGGAYATVCKALVTKASVFIPALSPYLLALYRVMKEKGTHERCIEQMQRLFTTKL"
  },
  {
    "reference": "MGTWAQFGLQDASSPLMEELTYFHDYALIVLTLITILVFYGLVSLLVSSNTNRFFFEGQELETIWTVIPALILILIALPSLQLLYLMDEVNDPFLTIKAFGHQWYWSYEYTDFNDLEFDSYMVPTSDVSFGNPRLLEVDNRLVLPMQNPIRVLVSSADVLHSWAVPSLGTKMDAVPGRLNQTTFFAARTGVFYGQCSEICGANHSFMPIVIESVPFNTFENWVTQYLEE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should incorporate a binding pocket that specifically recognizes Cu cation to enhance enzymatic efficiency.2. The protein must exhibit the following characteristics: The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.; Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation; Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)).3. The protein should have Helical domains to ensure proper insertion into the membrane.4. The designed protein should contain Mitochondrial intermembrane, Mitochondrial matrix topological domains.5. The designed protein should have a high specificity for Mg(2+), Cu cation binding, with minimal off-target effects.6. The ideal protein would be one that plays a critical role in ATP synthesis coupled electron transport through copper ion binding, cytochrome-c oxidase activity within mitochondrial inner membrane, respirasome.7. The protein should have a fast turnover rate for 4 Fe(II)-[cytochrome c] + 8 H(+)(in) + O2 = 4 Fe(III)-   [cytochrome c] + 4 H(+)(out) + 2 H2O. The designed protein sequence is ",
    "response#1": "MVFEFVLSLFNSVSGNHKIIGISYLWLAYWFGMIGFYMSVLIRTELGMSGLKIITMDTLEIYNLLFTLHGLIMVFFNIMTGLFGGIGNYLYPVLLGSCDVVYPRVNLYSLLLQPIGFVLVVSSVYLEIGSGTGWTLYPPLSTSLSNIGIDLIIFGLLAAGIASTLSSINFITTFASIKTIGFVIDRISPAAWSIVLTSFLLLLSLPVVTAVFLMVFFDRNHSTMFFESSNSGDPILYQHLFWFFGHPEVYIMILPGFGIISLLLSTYTTKEMFGNQTMILAMGSIALLGCLVWGHHMYTSGLEADTRGYFTTVTILIALPTGNKIFNWVTTLQCVESIKSLGLILFAVLFIVNFVIGGTTGVVLGNAGLDVVLHDTVYVVGHFHFVLSIGAIISLICFIVYIQRMLFGIILSNRLLSLMAPIFMIAVLFTFLPMHFTGFSPLPRRIPDYPDEMWGWNFICTLGATMMLVLKLTVLFIISLIVXXX",
    "response#2": "MVFEFVLSLFNSVSGNHKIIGISYLWLAYWFGMIGFYMSVLIRTELGMSGLKIITMDTLEIYNLLFTLHGLIMVFFNIMTGLFGGIGNYLYPVLLGSCDVVYPRVNLYSLLLQPIGFVLVVSSVYLEIGSGTGWTLYPPLSTSLSNIGIDLIIFGLLAAGIASTLSSINFITTFASIKTIGFVIDRISPAAWSIVLTSFLLLLSLPVVTAVFLMVFFDRNHSTMFFESSNSGDPILYQHLFWFFGHPEVYIMILPGFGIISLLLSTYTTKEMFGNQTMILAMGSIALLGCLVWGHHMYTSGLEADTRGYFTTVTILIALPTGNKIFNWVTTLQCVESIKSLGLILFAVLFIVNFVIGGTTGVVLGNAGLDVVLHDTVYVVGHFHFVLSIGAIISLICFIVYIQRMLFGIILSNRLLSLMAPIFMIAVLFTFLPMHFTGFSPLPRRIPDYPDEMWGWNFICTLGATMMLVLKLTVLFIISLILG",
    "response#3": "MVFEFVLSLFNSVSGNHKIIGISYLWLAYWFGMIGFYMSVLIRTELGMSGLKIITMDTLEIYNLLFTLHGLIMVFFNIMTGLFGGIGNYLYPVLLGSCDVVYPRVNLYSLLLQPIGFVLVVSSVYLEIGSGTGWTLYPPLSTSLSNIGIDLIIFGLLAAGIASTLSSINFITTFASIKTIGFVIDRISPAAWSIVLTSFLLLLSLPVVTAVFLMVFFDRNHSTMFFESSNSGDPILYQHLFWFFGHPEVYIMILPGFGIISLLLSTYTTKEMFGNQTMILAMGSIALLGCLVWGHHMYTSGLEADTRGYFTTVTILIALPTGNKIFNWVTTLQCVESIKSLGLILFAVLFIVNFVIGGTTGVVLGNAGLDVVLHDTVYVVGHFHFVLSIGAIISLICFIVYIQRMLFGIILSNRLLSLMAPIFMIAVLFTFLPMHFTGFSPLPRRIPDYPDEMWGWNFICTLGATMMLVLKLTVLFIISALMALTPATPMMQGIIDLHNDIFFFLIVILIFVLWMLVRALWHFHYKRNPIPERIVHGTTIEIIWTIFPSIILMFIAIPSFALLYSMDEVVDPAITIKAIGHQWYWTYEYSDYNSSDEQSLTFDSYMIPEDDLELGQLRLLEVDNRVVVPAKTHLRMIITSADVLHSWAVPSLGVKCDAVPGRLNQTSIFIKREGVYYGQCSELCGTNHGFMPIVVEAVSLDDYVSWKRHPFHIVRGTVAPLAVTLPLGFFVLNYFGVISSKLGLLIALSSFIGGLVIWTISIIFDSLYDQQHTYEVKRGLVMGMMMFIISEVMFFFSFFWSYFYISLSPNIAIGCVWPPYGLTVYSYMGLPLLNTVLLLLSGAILTDGYTILTEQKAVHEKNEKVLAVEEAFKNLMNLYKTKNSINTLTFVDERRDKFFGKESRQEDKIAEQKLIAISAGVKELRDLDWDLYFFENPENIEPNYKEPTNLSVIEYALITIYLKKRNKVIKTRLYFTLLCAVVFLACQGYEYFFAPFSMNDGIYGSLFFLLTGFHGFHVLVGSILIGIITIRFIVGNFNLLNVGTNFQIYKNKSTGFACTLFYWHFVDIVWIFLYIVIYWWG"
  },
  {
    "reference": "MTQNPQWLWQEVLTKLEQQLSRPTYETWIQPTAIQQWREDEIVLCAPNAFVLNHIQKYYGALITETIAELLQQPVKVRLTSPEGNTLAATQSFYSSRSGQSTRPGKKTPELNSKYTFSRFVVGPTNRMAHAAALAVAESPGRDFNPLVLCGGVGLGKTHLMQAIGHYRLDTQPDAKIFYVSTEQFTNDLIVAIRKDSLQTFREHYRTADILLVDDIQFIEGKEYTQEEFFYTFNTLHEAGKQIVLASDRPPHQIPGLQQRLSSRFSMGLIADIQPPDLETRMAILQKKAEAENLNLSRSVIEYIATHYTANIRELEGALLRAVTHIAISGLPMTVENLAPILNPTVEYAPAAPDVILQIAAEATGVSIEDLKGASRRREISTARQIAMYLMRQHTDLSLPRIGELFGGKDHTTVMYSCDKIGQLLTKNQKISQLVSQISDRINHHHQNL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that DnaA binds to ATP and to acidic phospholipids.2. The protein must have high specificity towards ATP binding.3. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding. The designed protein sequence is ",
    "response#1": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#2": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#3": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYF"
  },
  {
    "reference": "MRIDIITVLPEMLEGFFNCSIMSRAQKKGIAEIHIHNLRDYAYDRYRKVDDYPFGGFAGMVMKIEPIDRCISALKAEREYDEIIFTSPDGEQFNQPMANSLSLAKNLIILCGHFKGIDYRIREHFITKEISIGDYVLTGGELAAAVIADAVVRIIPGVISDEQSALSDSFQDNLLAAPVYTRPAEYKGWQVPDVLLSGHEAKIKEWELQQSLERTQRLRPDLLK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand.2. For general function, the protein need meet that Specifically methylates guanosine-37 in various tRNAs.3. A protein that localizes to cytoplasm and can perform tRNA (guanine(37)-N(1))-methyltransferase activity with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "MKRAVEKGIIEVHIYNIRDFSNNKHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQMSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGVLNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITKERR",
    "response#2": "MGKALEKDIININTINIRDFTEDKHKKVDDYPYGGGAGMVMSLQPIVDSLKRVKESNRGKVIFLGPRGKTFSHEMAKELSKEEELIFLCGHYEGIDERCYDYIDLEISLGDFILTGGEMACIPIVDSICRLIPGVLGKNESFMEESFYEGLLEYPHYTRPENFKGKEVPKILLSGHHENIRKWRKSKSLKITKKRRK",
    "response#3": "MGKALEKDIININTINIRDFTEDKHKKVDDYPYGGGAGMVMSLQPIVDSLKRVKESNRGKVIFLGPRGKTFSHEMAKELSKEEELIFLCGHYEGIDERCYDYIDLEISLGDFILTGGEMACIPIVDSICRLIPGVLGKNESFMEESFYEGLLEYPHYTRPENFKGKEVPKILLSGHHENIRKWRKSKSLKITKKRRR"
  },
  {
    "reference": "MNQSPNKRTKYIFVTGGVLSSLGKGLACSSIGALLELRGLKVSFLKFDPYINVDPGTMNPFQHGEVYVTDDGAEADLDLGHYERYTHVKTSKKNNYTTGQIYDSVISKERRGEYLGSTVQVIPHITDEIKSKIFALAEEVDVVIVEVGGTVGDIESLPFLEAIRQIRGEVGSDNTLYIHLTLVPYIETAGELKTKPTQHSVKELREIGIQPDILLCRTDRILPPNLKAKIALYCDVDKDAVITAKNVETIYEVPIVFHQEGLDEKIVEKLNIWTGRPNLKPWERVVRKIKHPRFETTIAVVGKYVSLVESYKSLTEALIHGGIANDARVNLVYVDSEEIEKNGPEDYLKGVQGILVPGGFGTRGIKGKIHAIQFAREKKIPFFGICLGMQCAVIEIAQHLAGLKGANSTEFDQNTPHPVICLLEEWVDRNRVVQKRDEATPKGGTMRLGAYPCRLKEGSLAFKAYGRKLISERHRHRYEFNNAYQEILERAGVRFSGVSPEQQLVEIIELKGHPWFLGCQFHPEFKSKPMAPHPLFREFIKASLLYGKKKPIKKESQKESEQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen2. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile, active site, Nucleophile; for glutamine hydrolysis to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).3. A protein with ATP binding, metal ion binding, CTP synthase activity functions is required to enable glutamine metabolic process, 'de novo' CTP biosynthetic process.4. A protein that can modulate the overall flux of metabolites through the Pyrimidine metabolism, CTP from UDP: step 2/2 pathway, enhancing or suppressing its activity as needed.5. The designed protein should contain one or more Glutamine amidotransferase, CTP synthase N-terminal domains that are essential for its function.6. The protein should be able to bind CTP, Mg(2+), UTP, ATP, L-glutamine ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "KYIFVTGGVVSGLGKGITAASLGRLLKARGLKVAAQKLDPYINVDPGTMSPYQHGEVFVTNDGAETDLDLGHYERFIDEDLNKYSNLTTGKVYWNVINKERSGEYLGETVQVIPHITNEIKSFVYEVGKKTDADVVITEIGGTTGDIESQPFIEAIRQISLEVGLSNCLFVHVTLIPYIQSSGEHKSKPTQHSVKELRSIGIMPNIIIARCDEPIDDSIRHKIAMFCNVKLDCVIQNITLPVLYEAPIMLDKENFSDIVCRELSLPKDNPPKILQDSYKSVYEALRHAAIANKVELNIMKIDSEEIEKNPENLTKVINQADGILVPGGFGSRGIEGKLAIIQIAREKKIPYFGICLGMQCMVIEYARNVLKIADANSSEFTETSNPVIHLMEDQYSIDRKGGTMRLGVYECNIKKGTLAEKIYGENIIFERHRHRYEFNNKYEDQFEKAGLIISGKYITKNLVEIVEYKDNPFGIGVQFHPEFKSKPFKPHPIFKSFIDNIYKSS",
    "response#2": "KYIFVTGGVVSGLGKGITAASLGRLLKARGLKVAAQKLDPYINVDPGTMSPYQHGEVFVTNDGAETDLDLGHYERFIDEDLNKYSNLTTGKVYWNVINKERSGEYLGETVQVIPHITNEIKSFVYEVGKKTDADVVITEIGGTTGDIESQPFIEAIRQISLEVGLSNCLFVHVTLIPYIQSSGEHKSKPTQHSVKELRSIGIMPNIIIARCDEPIDDSIRHKIAMFCNVKLDCVIQNITLPVLYEAPIMLDKENFSDIVCRELSLEVPKPDLTDWREMVERIRNPKRQVRIAIVGKYIEIKDAYLSIKEALKHAGIDSGCLVDIKWVEAEELEKMDIDEVREVFSDVEGILVPGGFGTRGSEGKIKAIRYARENKIPFLGLCFGFQLAVVEFARHVLGLEEAASAEVSETPYPVIEILPEQRNVRNMGGTMRLGDAEITLEEGTRAYEIYGKSKIIERHRHRYEVNPRYIEAIEKKGMVFSGKCGNRMEIAEIPDHPFFIGTQFHPEFKSRPDNPSPVFRAFIQACLESS",
    "response#3": "KYIFVTGGVVSSLGKGITAASLGRILKARGFKVTVQKFDPYLNVDPGEMSPFQHGEVFVTDDGAETDLDLGHYERFIDITVNKYSSATAGKIYSEVLRKERNGDYFGGTVQVIPHITNEIKSRMLRNGEKTDAEIVITEIGGTTGDIEGLPFIEAIRQLRTQLGRENVMFIHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDMIVVRTEYEMTEDLRNKIALFSDVSVENVIEAKDEDTIYNIVLSIQDQNMDDIVLKKLNLPCPEANLTEWENMLYKNKNPKAEVTIGLIGKYVELQDSYKSILEAFIHAGAENEVKVKVESIHSEHLTIKNSAKKLGHLDAVLVAPGFGERGVEGKIEAIKYVREQNIPFLGICLGMQMAVIEFSRNVLGLKNANSTEMNETTEYPVISLMESQKAVTDKGGTMRLGAWDCNILDNSIAKNVYKTNAIKERHRHRYEFNNDYKSQIEKAGMKATGLNPETGLVEIVEIPTNNWFVGVQYHPEYKSTVANPHPLFVAFVKAALDHQ"
  },
  {
    "reference": "MTKPKLLIVATGNPGKLQEMQAYLADLDWELQLKPPELEIEETGDTFAANACLKASQVAKVTRQWAIADDSGLQVDALGGAPGIYSARYGSTDANRIERLLRELGNEQNRAAQFVCAVAIARPDGEIALQAEGICRGEILSAPCGVGGFGYDPIFYVPQQQLTFAQMEPEIKQKYSHRGKAFAQLLPKLSQLA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must have high specificity towards ITP diphosphatase activity, ribonucleoside triphosphate phosphatase activity.2. The protein should be able to bind Mg(2+) ligand in a variety of conditions3. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "MRSIRR",
    "response#2": "GFSS",
    "response#3": "MPA"
  },
  {
    "reference": "MTKYIFVTGGVVSSLGKGITAASLGRLLKSRGYRVTIQKFDPYINIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLTRNSNVTAGRIYQSVIDKERRGDYLGRTVQVIPHITNEIKERVYRVGREENADFVITEIGGTVGDIESEPFLEAIRQVKKDVPRNDCLYIHVTLVPYIAAAGELKTKPTQHSVKELRSIGITPDILVCRSEHEVSKEMREKIAMFCDVDTDAVFQALTAKSIYEVPLLLQEQGMDKVVLKKLNMEDRPCDMKEWKDMVDKIMAPHKDKTKIGVVGKYVELPDAYISIVEALKHASFYNHADVEIEYINAVDVESPEANLDEIFKGIDGILIPGGFGDRGIEGMIRSAQYAREHKIPFFGICLGMQCAVIEYARNVCGMKKANSTEFDEATPYPVIYLMPEQMSVEIKGGTMRLGKYPCKLIAGSHAEEAYGTDLISERHRHRYEVNNDLKEELFSHGLVQAGTLPNGKLTEIVELPESEHPWYVGVQFHPELKSRPTNPHPLFKAFIAAALKNQKH",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be able to bind ATP ligand in a variety of conditions2. A key design requirement for the protein is the optimization of the Nucleophile; for glutamine hydrolysis to achieve high catalytic efficiency for H2O + L-glutamine = L-glutamate + NH4(+).3. A protein with glutaminase activity, ATP binding functions is required to enable 'de novo' CTP biosynthetic process, glutamine metabolic process.4. The designed protein have Glutamine amidotransferase domains with specific sequence motifs that are crucial for its activity.5. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates..6. The protein should be optimized for activity in Pyrimidine metabolism, CTP biosynthesis via de novo pathway pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MPDAYLSLLESLTHAGIANDARVNIKWVNAEELAEGDLETQLGDADGILVPGGFGIRGIEGKIKAAEYARTHNVPYLGICLGMQIAVIEYARHVAGLTGANSAEFDPYAPHKVIDLMPEQLEVEGLGGTMRLGDWPMELRAGTKIAELYGVPQGGTVRERHRHRYEVNPAYVGQLQDAGLVISGVTPGVQGRGAGLVESIEIPGHPFFVALQAHPEFKSRPMRPSPPFAGFVPDAYKSILEAFIHAGASNNVRVTIKLLRAEDAEAGSFDFKKEMQGVSGVLVAPGFGDRGIEGKISFIRYAREQNIPFFGICLGMQCATIEFARNVCGLHDANSTEFNKRTRFPVIDLMEQQKKVKEKGGTMRLGSYPCIIKEGSKAYDIYKKFLINERHRHRFEFNNTYRSALEEQGMVFSGTSPNGELVEIIELKDHRWFVGVQFHPELKSRVQQVHPLFHGFVQARKAATVTIAVVGKYTGLKDAYKSLIEXLTHGGIANKVRVNIRWLESESFEXPDQLHLLEGVNGILVPGGFGERGAEGKIAAAHFARTRNVPYFGICFGMQMAVIEAARNXAEVGKAGSTEFGATPEPVVGLMTEWLKEDELIQRGASGDLGGTMRLGAYDAILTPGSRVAEIYGTTKISERHRHRFEVNMSYRELLEAKGMVFSGTSPDGLLPEIVEIPEHPWFIGVQFHPELKSRPFDPHPLFESFLEASLA",
    "response#2": "MPDAYLSLLESLTHAGIANDARVNIKWVNAEELAEGDLETQLGDADGILVPGGFGIRGIEGKIKAAEYARTHNVPYLGICLGMQIAVIEYARHVAGLTGANSAEFDPYAPHKVIDLMPEQLEVEGLGGTMRLGDWPMELRAGTKIAELYGVPQGGTVRERHRHRYEVNPAYVGQLQDAGLVISGVTPGVQGRGAGLVESIEIPGHPFFVALQAHPEFKSRPMRPSPPFAGFVPDAYKSILEAFIHAGASNNVRVTIKLLRAEDAEAGSFDFKKEMQGVSGVLVAPGFGDRGIEGKISFIRYAREQNIPFFGICLGMQCATIEFARNVCGLHDANSTEFNKRTRFPVIDLMEQQKKVKEKGGTMRLGSYPCIIKEGSKAYDIYKKFLINERHRHRFEFNNTYRSALEEQGMVFSGTSPNGELVEIIELKDHRWFVGVQFHPELKSRVQQVHPLFHGFVRARRAAAVTIAVVGKYTGLKDAYKSLIEXLTHGGIANKVRVNIRWLESESFEXPDQLHLLEGVNGILVPGGFGERGAEGKIAAAHFARTRNVPYFGICFGMQMAVIEAARNXAEVGKAGSTEFGATPEPVVGLMTEWLKEDELIQRGASGDLGGTMRLGAYDAILTPGSRVAEIYGTTKISERHRHRFEVNMSYRELLEAKGMVFSGTSPDGLLPEIVEIPEHPWFIGVQFHPELKSRPFDPHPLFESFLDAALSGDH",
    "response#3": "MPDAYLSLLESLTHAGIANDARVNIKWVNAEELAEGDLETQLGDADGILVPGGFGIRGIEGKIKAAEYARTHNVPYLGICLGMQIAVIEYARHVAGLTGANSAEFDPYAPHKVIDLMPEQLEVEGLGGTMRLGDWPMELRAGTKIAELYGVPQGGTVRERHRHRYEVNPAYVGQLQDAGLVISGVTPGVQGRGAGLVESIEIPGHPFFVALQAHPEFKSRPMRPSPPFAGFVPDAYKSILEAFIHAGASNNVRVTIKLLRAEDAEAGSFDFKKEMQGVSGVLVAPGFGDRGIEGKISFIRYAREQNIPFFGICLGMQCATIEFARNVCGLHDANSTEFNKRTRFPVIDLMEQQKKVKEKGGTMRLGSYPCIIKEGSKAYDIYKKFLINERHRHRFEFNNTYRSALEEQGMVFSGTSPNGELVEIIELKDHRWFVGVQFHPELKSRVQQVHPLFHGFVGGRVKDLPGVRYHIVRGATDKHGRVDRKKYGRK"
  },
  {
    "reference": "METLVGGLLTGEDSLISMSNDVSCLYVYDGPMRVFSQNALMPTLQSVKRSDQFSKGKTKRFIIDLFGMKRMWDIGNKQLEDENLDETVGVADLGLVKYLINNKYDEAEKTSLRKSMEEAFEKSMNEEFVVLNKGKSANDIISDTNAMCKFCVKNWIVATGFRGRTMSDLIEHHFRCMQGKQEVKGYIWKHKYNERLKRKQLSKEEVKFDREEYTSRSFRLLSFLKNSERTKLEPRAVFTAGVPWRAFIFVLEQTMLVVNKLDPNSVIWMGSDAKINTTNSRIKEIGMKNQGQTLVTLTGDNSKYNESMCPEVMMVFLRELGIKGPMLEVLDYALWQFSQKSVKPVAPIKKRTGKSTVVIKADSVKECRDAFNEKELELIQGVEWMDDGFVRVRRGMLMGMANNAFTTASTIASSFSFTPEAVYTLQSSDDFVTGSCGRDVQHARQRLEMALKVSKAAGLNVSQKKSFYVEGTTFEFNSMFVRDGKVMANGGNFENMTVPGGLGPSTDLFVVGKQARNSMLRGNLSFSQAMEMCKIGITNVEKVYYGNRKYQELKNEIREKCGEETMSIPESMGGDRKPRPWELPQSFDGIALKEAVNRGHWKAAKYIKSCCSIEFDEEGDQSWDTSKTALVVIRKNETDMRRRTVKTRNPKDKIFNDAMNKAKRMYETVVDRNPLLGLKGKGGRLTVKDLKARKLIDEVEVVKKKKHV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. We need a protein that can perform a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +   RNA(n+1) with high efficiency.2. The designed protein should contain one or more RdRp catalytic domains that are essential for its function.3. For general function, the protein need meet that Binds the promoter sequence of the encapsidated viral RNA; RNA-dependent RNA polymerase which is responsible for replication and transcription of virus segments; Displays an endonuclease activity involved in cap-stealing; Cleaves cellular pre-mRNA to generate primers for viral transcription .4. The designed protein should be optimized for RNA-dependent RNA polymerase activity, RNA binding activity in order to improve viral RNA genome replication. The designed protein sequence is ",
    "response#1": "MDEKISSLKDFVRKQIPDRPEFFYQKEALLSQVEVSLILTEGFKLMSCLVEVESCEKNSCVHNSEQKFVDTILAENGIVGPTLPKVMPDGYRYFNKTLLLLEVFVRVRPDEFEKKWKSDMGKLLSLKDDLLRCGISLVPIVDGRCSYNTSIIPEWATERFRWLLIELLKESE",
    "response#2": "PDTSSNRFYTLESKEWSTNSKGWWWKLPDALKDMGIFGENMFYHFLGRSGYTVHVQCNASKFHQGTLIVAMIPEHQLASATTGNATAGYNLTHPGEGGRDVSLSNRSDLSKQPSDDAWLNFDGTLLGNITVYPHQLINLRTNNSATIIVPYVNAVPMDSMVRHNNWSLVIIPICPLESLPSSTVPITISISPMCAEFSGARAGEDMVETRYVQTSQTLDEMSMESFLGRSGCIHISKLVVEYEGYDDTKNFKTWKINLQEMAQIRRKFEMFTYTRFDSEITLVPSIAAKGDDIGHVVMQYMYVPPGAPVPKKRDDYTWQSGTNASIFWQYGQTYPRFSLPFLSIASAYYMFYDGDRINELKDLVRRWVPDEEAYTEQKTIVLSQVNPSSVITEGLKLLSMLIEIDSCLKHGCVFNKNKTVNQILKDHKIVGPTLPDVVPDGFKVSGSTIILLETFVRVNQESFEQKYKYDFEKLMQLSKDLNKCGLILVPVIDGRSNYYVDRFPDWVIERIRWLLLKLMF",
    "response#3": "LSELCQVDTIVPINHTTSTTSSVNMYTIQLTKEQPGTEVFAMRVDVASQPLATTLIGEISSYYTNWTGSLRFSLMFCGSAGTTLKMLVAYTPPGIAKPETRKDAMLGTHVVWDVGLQSTMSMIVPWISASXXXXTTPDTFSQAGYITCWYQTSLVTPPSTPETARMLCFVSGCKDFCLRMARDTC"
  },
  {
    "reference": "MTKTKIIGILNVTPDSFSDGGKYNSVDKAIARAKEMIDEGVDIIDVGGVSTRPGHTEVSLEEEMERVVPVVEQLVKLDVQISVDTYRSEVAEACLKLGATMINDQWAGLYDPKIFDVVSDYNAEIVLMHNGDGQREQPVVEEMLLSLLTQANKAEMAGIEKGNIWLDPGIGFAKSRSEEKEVMARLDELVATEYPVLLATSRKRFIKEMIGKETTPAERDEATAATTVYGIMKGIQAVRVHNVDLNVKLAQSIDFLKENEHERHHLS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a high specificity for (7,8-dihydropterin-6-yl)methyl diphosphate binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "SRDPELAGAISQELTRQRDEIELIASENIVSLAVLQAQGSVMTNKYAEGYPGRRYYGGCQYVDIAEQLAIDRAKQLFGAGYANVQANSGSQANQSVFLALLQPGDTFLAMDLSAGGHLTHGARPNMSGKWFKAVHYGVRQQDSQLDYDQMEELAREHKPKLIIAGGSAIPRQIDFARFRKMADEIGAYLMVDMAHFAGLVAGGQHPNPLEHAHVVTTTTHKTLRGPRGGMILTNDEAIAKKINSAIFPGLQGGPLMHVIAGKAVAFGEALRPEFADYAAHVVANAKVLAETLMKGGIDIVTGGTDTHLMLVDLRPKGVTGKDAEAALGRAHITCNKNGIPFDPQPPTVTSGVRLGTPAGTTRGFGEAEFREIGELIAEVL",
    "response#2": "VQVIGVLNVTDNSFSDGGRYLDPDDAVQHGLAMVAEGAAIVDVGGESTRPGAIRTDPRVELSRIVPVVKELAAQGITVSIDTTRADVARAALQSGARIVNDVSGGRADPAMAPLVAEAGVAWVLMHWRLMSAERPYEAPNYRDVVAEVRADLLAGVDQAVAAGVDPGSLVIDPGLGFAKTGQHNWALLNALPELVATGVPILLGASRKRFLGRLLAGADGAVRPPDGRETATAVISALAALHGAWGVRVHDVRASVDALKVL",
    "response#3": "SRDPELAGAISQELTRQRDEIELIASENIVSLAVLQAQGSVMTNKYAEGYPGRRYYGGCQYVDIAEQLAIDRAKQLFGAGYANVQANSGSQANQSVFLALLQPGDTFLAMDLSAGGHLTHGARPNMSGKWFKAVHYGVRQQDSQLDYDQMEELAREHKPKLIIAGGSAIPRQIDFARFRKMADEIGAYLMVDMAHFAGLVAGGQHPNPLEHAHVVTTTTHKTLRGPRGGMILTNDEAIAKKINSAIFPGLQGGPLMHVIAGKAVAFGEALRPEFADYAAHVVANAKVLAETLMKGGIDIVTGGTDTHLMLVDLRPKGVTGKDAEAALGRAHITCNKNGIPFDPQPPTVTSGVRLGTPAGTTRGFGEAEFREIGELIAETLDAPADLAQVRRRVAALTERFPLYA"
  },
  {
    "reference": "MVSNNAVIIKKYLDSTAGYPVIGEHLAFEKREFDLENAQVDEETPVLLKNIYTSVDPYLRMRMQSPKHASYIPPLELGKPFYNSTVAKVVKSTLDQYKPGMDVVFVSGWEEYTFVSKQALGFLQPINNPYKLPLIDFVGSLGMPSQTAYCGLKHIGKPKAGETIYISAASGAVGQMAGQLAKAMGLHVVGSVGSDEKFKICLDSGYDSVFNYKKESPFKALPRLCPKGIDIYFENVGGETMDAVLENMNLQGRIIFCGAISQYNNPNPYRVKNLGMVLVKSLTIQGFIVANILPQYQEQYFEEMPKLIAEGKIKYKCDVYDGLESAPEAFIGMLQGKNSGKTIVKIADE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein can be targeted to cytosol, nucleus and perform oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor. The designed protein sequence is ",
    "response#1": "WLKPEHRSSVPCQDPITGENLASCLQAQSEDVAAAVEAARASLENWSTQPGAIRAQHLTRLAKVIQKHQRLLWTLESLVTGRAVREVRDRDVPLAQQLLQYHAVQAHTQEEALAGWEPMGVIGLILSPTFPFLDMMWRICPALAVGCTVVVLVPPASPTPLLLAQLAGELGPFPGILNVISGPASLGPVLAAQPGVQKVAFCGAIEEGRALRRTLAGWVPELGLALGAESLLLLTEVADVDSAVEGIVDAAWSDRSPGGLRLLIQEAVWDETMRRLQERMGRLRCGHGLDGAVDMGARGAAARDLAQRYVSEAQSQGAQVFQAGSEPSDSPFFPPTLVSDLPPASPCTQAEVPWPLVVASPFRTAKEALAVANGTPRGGSASVWSERLGQALELAYGLQVGTVWINAHGLRDPAVPTGGCKESGSSWHGGQDGLYEYLY",
    "response#2": "WLKPEHRSSVPCQDPITGENLASCLQAQSEDVAAAVEAARASLENWSTQPGAIRAQHLTRLAKVIQKHQRLLWTLESLVTGRAVREVRDRDVPLAQQLLQYHAVQAHTQEEALAGWEPMGVIGLILSPTFPFLDMMWRICPALAVGCTVVVLVPPASPTPLLLAQLAGELGPFPGILNVISGPASLGPVLAAQPGVQKVAFCGAIEEGRALRRTLAGWVPELGLALGAESLLLLTEVADVDSAVEGIVDAAWSDRSPGGLRLLIQEAVWDETMRRLQERMGRLRCGHGLDGAVDMGARGAAARDLAQRYVSEAQSQGAQVFQAGSEPSDSPFFPPTLVSDLPPASPCTQAEVPWPLVVASPFRTAKEALAVANGTPRGGSASVWSERLGQALELAYGLQVGTVWINAHGLRDPAVPTGGCKESGSSWHGGQDGLYEYLY",
    "response#3": "WLKPEHRSSVPCQDPITGENLASCLQAQSEDVAAAVEAARASLENWSTQPGAIRAQHLTRLAKVIQKHQRLLWTLESLVTGRAVREVRDRDVPLAQQLLQYHAVQAHTQEEALAGWEPMGVIGLILSPTFPFLDMMWRICPALAVGCTVVVLVPPASPTPLLLAQLAGELGPFPGILNVISGPASLGPVLAAQPGVQKVAFCGAIEEGRALRRTLAGWVPELGLALGAESLLLLTEVADVDSAVEGIVDAAWSDRSPGGLRLLIQEAVWDETMRRLQERMGRLRCGHGLDGAVDMGARGAAARDLAQRYVSEAQSQGAQVFQAGSEPSDSPFFPPTLVSDLPPASPCTQAEVPWPLVVASPFRTAKEALAVANGTPRGGSASVWSERLGQALELAYGLQVGTVWINAHGLRDPAVPTGGCKESGSSWHGGQDGLYEYLY"
  },
  {
    "reference": "MAAPRAVLHLGAREWNGRARRIHGMSELVTPDSSREKKRTLLQFLSDHFQDIQTLREYLLQKQISKVNRENRSFTNIQEKYGPYVAGAVFILKQGGAVKFQDKEEWIRPNNRSHFLAEIQKFQNVPVEAVDASGCAINYQGLSNLLPLKELRSLSLQRCPNLDDWCLSRLYLLAGSLQELSLAGCPRISERGLACLHHLQNLRRLDISDLPAVSHPGLTQILVEEMLPHCEVLGADWAQNLKLEPDKQPPDTSTPLSS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Involved in the assembly of the distal region of complex I.2. The designed protein should be expressed specifically in the SCF ubiquitin ligase complex The designed protein sequence is ",
    "response#1": "GMRNHPLDLRVQFTASACALNLTRQGLAKGMPVRLLSEVTCLLFKALKNFPHYQQLQKNCLLSLTNSRILVDVPFDRFDAAKFVMRWLCKHENPKMQTMAVSVTSILALQLSPEQTAQLEELFMAVKELLAIVKQKTTENLDDVTFLFTLKALWNLTDGSPAACKHFIENQGLQIFIQVLETFSESAIQSKVLGLLNNIAEVRELSSKLVTEDVLKHINSLLCSREMEVSYFAAGIIAHLTSDRQLWISRDFQRRTLLQDLHATIQNWPSSSCKMTALVTYRSFKTFFPLLGNFSQPEVQLWALWAMYHVCSKNPSKYCKMLVEEEGLQLLCDIQEHSEATPKAQQIAASILDC",
    "response#2": "GMRNHPLDLPVQFTASACALNLTRQELARGMPVRLLAEITDLLFKATKNFPYYQQLQKNCLLSLTSSRILMDVPFDRFDAAKLALRWVCRRESPKLRTMAVSITSILALKLSPEEMGQLQEELIMAIKELLTIIRQKLAENLDDVTFLFTLKALWNLTDECPLACKYFMENEGLATVIRVLETFSISVIQSKVLGLLNNVAEVRELSSKLVTEDVIERIISLLHSSNLEVSFLAAGVLAHLTCDRQHWLSRDLQRTDLLRYLHLAIQNWPSSRCDMSVLVTYRSFKAFSPLLVNFSQPEVQRWALWAIHHVCSKNPL",
    "response#3": "GMRNHPLDLRVQFTASACALNLTRQGLAKGMPVRLLSEVTCLLFKALKNFPHYQQLQKNCLLSLTNSRILVDVPFDRFDAAKFVMRWLCKHENPKMQTMAVSVTSILALQLSPEQTAQLEELFMAVKELLAIVKQKTTENLDDVTFLFTLKALWNLTDGSPAACKHFIENQGLQIFIQVLETFSESAIQSKVLGLLNNIAEVRELSSKLVTEDVLKHINSLLCSREMEVSYFAAGIIAHLTSDRQLWISRDFQRRTLLQDLHATIQNWPSSSCKMTALVTYRSFKTFFPLLGNFSQPEVQLWALWAMYHVCSKNPSKYCKMLVEEEGLQLLCDIQEHSEATPKAQQIAASILDL"
  },
  {
    "reference": "MSGVRIPLFPRFIPHGTVKLESAALKKLNSLELSGGYAVAVSGGVDSITLLNLVAALHKTQATSRPVVLTGNHGFRAEADHETRFVQKRAVALGLDCEILRWNRHRTSSKSQETAREIRYSLLHQWCTEHSVKFLLTAHNKSDQAETVLMHLERGSGIDGLSGMHERSVFGDITIYRPLLGFTRQEILEYATQKQLSWVEDPSNQDPKYRRTFFRNLIAESKNPGVVVDRLCRTTSHMHRALACILHYVRSSLDYCLEFSPLGFITVKSQELRSVPEEIASRLLLLSLMAMGGKDHKPRYSAFWPILTKILQGQDFTPRTLHGCKVRKEPDGNFSIVRELARIETKIGVKTIAETIQWDRRFAIKIMCTHNASAAETTSCSAKHAPNPSEETQNLYITPLGNGSLPEHLVHVNRDVACSLPVLAHGDKVLAYPWQNHNIGVSPTISVEEVLVRRGVINLICHQLYR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be optimized for ligase activity, forming carbon-nitrogen bonds, ATP binding activity in order to improve tRNA modification.2. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.3. The protein should have a low Km value for ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP +   diphosphate + H(+) + lysidine(34) in tRNA(Ile2).4. The protein must exhibit the following characteristics: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. The designed protein sequence is ",
    "response#1": "AVSGGQDSMALLVLLQELQRLHHWPLNIWHGDHGWHSGSAVIAADLRSWCQQRDLPIQVDQAPQGSTASEASARHWRYSQLQQRAEELGADVVTGHTASDRAETLLLQLARGTDLAGLGALRPVRPLFNDSPDGAQLRRPLLGFSRADTAAVCRDLQVPIWHDPSNQSPAFARNRIRAEVLAGPRRPR",
    "response#2": "AVSGGQDSMALLVLLQELQRLHHWPLNIWHGDHGWHSGSAVIAADLRSWCQQRDLPIQVDQAPQGSTASEASARHWRYSQLQQRAEELGADVVTGHTASDRAETLLLQLARGTDLAGLGALRPVRPLFNDSPDGAQLRRPLLGFSRADTAAVCRDLQVPIWHDPSNQSPAFARNRIRAEVLERAF",
    "response#3": "ILLAVSGGPDSVCMLYIMHKLSREMGFSLRLAHLNHGLRAEESDRDAAYVTELASALGLPLCQQKVSVKAYQAEHHLSLEEAAREMRYAFLCRTAAEFGSAAVAVGHTLDDNIETVMLHLVRGSGTRGLQGLRPVLNRTIAGAGCLRVIRPLLCLGRAETQVYCREAGLLPRQDITNLSTEPLRNRIRLEVLEWW"
  },
  {
    "reference": "MTALQSWYDAEATEALALDPIELAISEIAAGRPVVVVDDENRENEGDLVIAAEKATPEIVAFMMSECRGLICAPMEGPELDRLELPQMVENNTESMRTAFTVSVDATAAHGVSTGISAADRATTLRLLAAGESVAGDFVRPGHIFPLRARPGGVLVRNGHTEAGVDLARLAGLRPAAAIVEIAGEDGTMLRLPELVPFARKHGLAIISIEDLIAYRQRSTPTVRREAETRLPTAHGEFTAYGYRSTVDGVEHIALVAGEIGDGEDVLVRVHSECLTGDIFHSLRCDCGPQLEASLEQITEAGRGVVIYLRGHEGRGIGLLSKLRAYELQERGRDTLDANLELGLPADSRDYAAGAQMLQDLGVRSLRLMTNNPEKTAALVRHGLKVNGREPVAVQAGEHNLRYLRTKRDRMGHDLPWLDPAAGTGSTGAAYATPPAGASTCGHQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein with GTP binding, manganese ion binding, GTP cyclohydrolase II activity, magnesium ion binding functions is required to enable riboflavin biosynthetic process.2. The protein with the reaction GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate, the Nucleophile; for GTP cyclohydrolase activity must be optimized to ensure efficient catalysis.3. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.4. For general function, the protein need meet that Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.5. The protein should be optimized for activity in riboflavin biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway and exhibit minimal cross-reactivity with other metabolic pathways.6. The Mg(2+), D-ribulose 5-phosphate, GTP, Zn(2+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "VEVDMPTKYGHFHLIPFLQKSNGLEHIALIKGKWDEDEEILVRVHSSCMTGDIFGSLRCDCGDQIHKAMELIEKEGRGVIVYLMQEGRGIGLMNKMKAYKLQEEGLDTVEANIELGFAADERDYGIGAQILRQLNVRKMRLLTNNPVKRIGLEGYNLQIVGNVVETKMPTKYGDFKAIGYVNKLNGEHHVALVKGEIKKDEPVLVRVHSECLTGDAFGSKRCDCGEQLSMAMEKINKEGAGVLLYMRQEGRGIGLLNKLKAYKLQDEGLDTVEANIALGFEADLRDYGIGAQILSDLGIKKIRLMTNNPKKVSGLSGHDIEIVERVVVETKMPTKYGDFKAIGYVNKLNGEHHVALVKGEIKKDEPVLVRVHSECLTGDAFGSKRCDCGEQLSMAMEKINKEGAGVLLYMRQEGRGIGLLNKLKAYKLQDEGLDTVEANIALGFEADLRDYGIGAQILSDLGIKKIRLMTNNPKKVSGLSGHDIEIVERVEAEAKLPTKYGCFKVVGYRNKINGKENLAIVKGNIQNEKEVLVRVHSKCLTGDVFGSLRCDCGNQLSAALKAIEKHGSGVLLYMDQEGRDIGLLNKLKAYELQEKGMDTVEANLELGFPPDLRDYWGAAEILKDLGVKRIKLMTNNPDKIEKLQKYGIDVVKRVREVEVKMPTEFGVFKAVAYTNDVDNKEHVALVKGEIDSSQPTLVRVHSECLTGDVFHSHRCDCGPQLEAALRQIDSEGSGVLLYMRQEGRGIGLINKLKAYKLQEEGLDTVEANQKLGFAPDLRDYGIGAQILKDLGVSQIKLLTNNPKKIRGLEGYGLEVVERV",
    "response#2": "MNRVAETSIPNRYGTWRAYGYTSSLDGGEHLALVLGEGLEENVLVRAHSECLTGDVLGSLRCDCGVQLEHAMEAIAAEGRGVIVYLRGHEGRGIGLVAKLRAYSLQDNGSDTVDANLELGLPVDAREFSNAGQILGDLGVKSVRLLTNNPAKLRGMDGYGIKVLGRAHALDATRPLERVAEARIPLSAGTFRAVGYDSLLDGIEHIAFVYGEIGDGEDILVRVHSECLTGDVFGSLRCDCGPQLEAALEAVAKEGRGVVLYIRGHEGRGIGLLHKLQAYQLQDAGADTVDANLQLGVPADARDYGTGAQILCDLGVRSMRLLTNNPAKRVGLEGYGLRVTGRHRLDVTTPLDGRAGERVAEARIPLSAGTFRAVGYDSLLDGIEHIAFVYGEIGDGEDILVRVHSECLTGDVFGSLRCDCGPQLEAALEAVAKEGRGVVLYIRGHEGRGIGLLHKLQAYQLQDAGADTVDANLQLGVPADARDYGTGAQILCDLGVRSMRLLTNNPAKRVGLEGYGLRVTGRHTLDLTAPAGHALGLERVAEARIPLSAGTFRAVGYDSLLDGIEHIAFVYGEIGDGEDILVRVHSECLTGDVFGSLRCDCGPQLEAALEAVAKEGRGVVLYIRGHEGRGIGLLHKLQAYQLQDAGADTVDANLQLGVPADARDYGTGAQILCDLGVRSMRLLTNNPAKRVGLEGYGLRVTGRHRLDVATPA",
    "response#3": "MTEAEAKLPTKYGCFKVVGYRNKINGKENLAIVKGNIQNEKEVLVRVHSKCLTGDVFGSLRCDCGNQLSAALKAIEKHGSGVLLYMDQEGRDIGLLNKLKAYELQEKGMDTVEANLELGFPPDLRDYWGAAEILKDLGVKRIKLMTNNPDKIEKLQKYGIDVVKRVSTRIPLQNKGDFNMTVFANELDCAEHFALVKPPAFANRVPLVRIHSECITGDVFGSCKCDCGKQLEQSLSLIAAEGGILIYLRQEGRGIGLANKLKAYALQEQGLDTVDANHQLGLPVDGRDYAIAYQILKYFGIDAIRLLTNNPSKIAAVDRYGIKVMERVPATTHSDQPVEVDMPTKYGHFHLIPFLQKSNGLEHIALIKGKWDEDEEILVRVHSSCMTGDIFGSLRCDCGDQIHKAMELIEKEGRGVIVYLMQEGRGIGLMNKMKAYKLQEEGLDTVEANIELGFAADERDYGIGAQILRQLNVRKMRLLTNNPVKRIGLEGYNLQIVGNREVEARVPTEHAGEFKAVVYSNSVDHLDHLALVKGDISKAERVLVRVHSECLTGDVFGSARCDCGLQLNAAMKMIDQEGCGIVLYMRQEGRGIGLVNKLKAYCLQDDEGVDTVEANHRLGFKSDLRDYGIGAQILRDLGVKKMAILTNNPKKIVGLEGYGIEVVERLPATEHSDQPAKLVMI"
  },
  {
    "reference": "MTKLYNVAVVGATGAVGEAMLAGLIERKFPVDTVYAIASERSVGKTIDFGRDTLDVLDIATFDFTDVDLALFSAGNEVSKQYAPIAAAHGCVVIDNSSCFRYDADIPLVIPEVNPECIADYTRQGIIANPNCSTIQLLVALKPLHDAVGITRINVATYQSVSGTGKEAISELINQLGQLLNGRPVKPSVYPQQIAFNVLPHIDAFQDNGYTREEMKMVWETQKILGDDTILVNPTAVRVPVLYGHSEAIHVEFKSPLTAAEAKKLLKKAPGVVVVDDKNKYPTVISHATGHDEVFVGRIRQDISHPNGLDLWVVADNVRKGAATNAIQIAELLCRDYL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should consider the endogenous proteins and enzymes present in the L-threonine biosynthesis pathway to avoid interference with native metabolic processes.2. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..3. The protein with the reaction L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH, the Proton acceptor must be optimized to ensure efficient catalysis.4. The protein contains novel Semialdehyde dehydrogenase NAD-binding domains that confer a unique function or activity.5. The designed protein should have NADP binding, protein dimerization activity to facilitate diaminopimelate biosynthetic process, isoleucine biosynthetic process. The designed protein sequence is ",
    "response#1": "MSKVGFVGWRGMVGSVLMDRMREENDFANIDPVFFTTSQHGKPGPIVSSDPGKPVAALQDAYSIEALAAMDIIISCQGGDYTHKVIEPLRESGWNGYWVDAASSLRMKNDSVIVLDPVNRPVIDQALAKGGRNWIGGNCTVSCMLMKVGFVGWRGMVGSVLMDRMREENDFANIDPVFFTTSQHGKPGPIVSSDPGKPVAALQDAYSIEALAAMDIIISCQGGDYTHKVIEPLRESGWNGYWVDAASSLRMKNDSVIVLDPVNRPVIDQLFPLHIEYKINRVIVSTYQSVTGTGKKALDQLYQEKNGNYSCNKVYPYPIYQNVLPHCDHFTDKEYTIEEMKLINETKKIMNDYNLAITATAVRVPVIGGHSESVNITFVKKPSINRVREILLKTKGIILQDQPKKNIYPTPLYAHGKDEVFVGRIREDFSFKNSIHIWIVSDNLRWGAAEPLRRMLRLLLEA",
    "response#2": "MNSLAIVGATGQVGTVMREILIDRSFSIRDLRLFATARSAGTSIEFGGHDIIVEDVATADPSGIDVALFSAGATGSRAHAPRFAEAGALVIDNSSAWRMDPEVPLVVSEVNPHAIGEIPKGIVANPNCTTMMAVMAIHLNPLHKKYGIKRVIISTYQSITGTGVKAVEQLNNEQQGIKGEMAYPYPIDKNCLPHCDVFTENGYTKEEIKLMTEPKKIMQDDSFSLTATAVRVPVVGGHSESVNIEFKTNVDVKEIRKILHETSGVVLQDNTDTNTYPMPIYAADKDEVFVGRIRKDESQPNTINLWIVADNLRKGAALNAVQIAELLVRR",
    "response#3": "MTRVGLVGWRGMVGSVLMQRMREERDFDLIEPVFFTTSQVGGKGPDIGRDIPALKDARNIDELKAMDAIISTQGGDYTTEIFGQLRSAGWKGYWIDAASTLRMEKDSVIILDPVNQHVIKDALAAGGRNWIGGNCTVSCMLMGVGLAPLHKKYTMRRVIVSTYQSVSGTGVKAVQQLDNEIAGIKGEMAYPYPINRNALPHCDVFEENGYTKEEMKLAREPQKILNDKTFSVSATAVRIPTAGGHSESVNVEFENDFDLAQVRKLLHETPGIVVQDNPATNTYPMPIYAHDKDDVFVGRIRRDETQPNTLNMWIVSDNLRWGAAEPIRRMLRILVE"
  },
  {
    "reference": "MDIDHGRESDGEMVGTIASCGLLLHSLLAGLGRRAAGFARKVGGAAREDPRRVAHSLKVGLALALVSVVYFVTPLFNGLGVSAIWAVLTVVVVMEYTVGATLSKGLNRALATLVAGCIAVGAHQLAELAERCGDQGEPIVLTVLVFFVASAATFLRFIPEIKAKYDYGVTIFILTFGLVAVSSYRVEELIQLAHQRFYTIAVGVFICLCTTVFLFPVWAGEDVHKLASGNLDKLAQFIEGMEFNCFGENSVANNFGGKDSPQMHKSVLNSKATEDSLCTFAKWEPRHGQFRFRHPWSQYQKLGTLCRQCASSMEALASYVITTSKTQCPAAANPELSCKVRKTCGEMSLHSSKVLRDLAMATRTMTVPSPVNITMATAVKAAESLRSELAENTALLQVMHVAVTATLLADLVDRVKEIAECVDVLARLAHFKNPEDTKNVVVSTVSRGIDEPLPDVVIL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Malate transporter critical for aluminum tolerance.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The protein should have a specific function in malate transport, anion transmembrane transport and should be localized to plasma membrane, plant-type vacuole membrane in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "MVLSSFFVHILIMGSQMALGVLNVEWLEEFHQSRGLTAWVSSLSMGITLIVGPFIGLFINTCGCRQTAIIGGLVNSLGWVLSAYAANVHYLFITFGVAAGLGSGMAYLPAVVMVGRYFQKRRALAQGLSTTGTGFGTFLMTVLLKYLCAEYGWRNAMLIQGAVSLNLCVCGALMRPLSPGKNPNDPGEKDVRGLPAHSTESVKSTGQQGRTEEKDGGLGNEETLCDLQAQECPDQAGHRKNMCALRILKTVSWLTMRVRKGFEDWYSGYFGTASLFTNRMFVAFIFWALFAYSSFVIPFIHLPEIVNLYNLSEQNDVFPLTSIIAIVHIFGKVILGVIADLPCISVWNVFLLANFTLVLSIFILPLMHTYAGLAVICALIGFSSGYFSLMPVVTEDLVGIEHLANAYGIIICANGISALLGPPFAGWIYDITQKYDFSFYICGLLYMIGILFLLIQPCIRR",
    "response#2": "PWIFGLSLLGLTPLAERVSFLTEQLAFYTGPTLGGLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIANIRREQRFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIVMLISYIAYLVFQLLR",
    "response#3": "MVLSSFFVHILIMGSQMALGVLNVEWLEEFHQSRGLTAWVSSLSMGITLIVGPFIGLFINTCGCRQTAIIGGLVNSLGWVLSAYAANVHYLFITFGVAAGLGSGMAYLPAVVMVGRYFQKRRALAQGLSTTGTGFGTFLMTVLLKYLCAEYGWRNAMLIQGAVSLNLCVCGALMRPLSPGKNPNDPGEKDVRGLPAHSTESVKSTGQQGRTEEKDGGLGNEETLCDLQAQECPDQAGHRKNMCALRILKTVSWLTMRVRKGFEDWYSGYFGTASLFTNRMFVAFIFWALFAYSSFVIPFIHLPEIVNLYNLSEQNDVFPLTSIIAIVHIFGKVILGVIADLPCISVWNVFLLANFTLVLSIFILPLMHTYAGLAVICALIGFSSGYFSLMPVVTEDLVGIEHLANAYGIIICANGISALLGPPFAGWIYDITQKYDFSFYICGLLYMIGILFLLIQPCIRS"
  },
  {
    "reference": "MQRIASIHRQTAETDVHIHLNLDGHGQYNVKTGIGFFDHMLDQLFRHSLIDCDVTVKGDLHIDPHHTIEDCALALGQAVQQALGDKAGITRFSHAYAPMDEALSRVAIDISGRPYLVWHSAFTQPRLGDLDTEMLEHWFLSFAQGAGLTLHVETLYGRNNHHIAESLFKALALALRQAVAIDAGKQGRIPSTKGSL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must be optimized for imidazoleglycerol-phosphate dehydratase activity. The designed protein sequence is ",
    "response#1": "IRNQELGELLMLHHQHRNPTTPAGFTNEMLINDSVVHEFDAIRFFTGEEITSVQVRLGKVTKNAPAGQHDPQHVLIETESGVLADVEIYVNAKFGYEVATQASFEDGIVSIGGDKGPYTRSAGRWGGNVTPGFEERFGAAYDVEIQSWVDAALRGEIGGPSAWDGYATAACCEAGVEAQKNGEKVAM",
    "response#2": "IRNQELGELLMLHHQHRNPTTPAGFTNEMLINDSVVHEFDAIRFFTGEEITSVQVRLGKVTKNAPAGQHDPQHVLIETESGVLADVEIYVNAKFGYEVATQASFEDGIVSIGGDKGPYTRSAGRWGGNVTPGFEERFGAAYDVEIQSWVDAALRGEIGGPSAWDGYATAACCEAGVEAQKNGEKVAA",
    "response#3": "IRNQELGELLMLHHQHRNPTTPAGFTNEMLINDSVVHEFDAIRFFTGEEITSVQVRLGKVTKNAPAGQHDPQHVLIETESGVLADVEIYVNAKFGYEVATQASFEDGIVSIGGDKGPYTRSAGRWGGNVTPGFEERFGAAYDVEIQSWVDAALRGEIGGPSAWDGYATAACCEAGVEAQKNGEKVAM"
  },
  {
    "reference": "MGENDESEIIEHHDDEEMEKRRPPRTHAQSFLESVASSVKEGHSSTQSSHRLLAYSDALISIIATVMILPVAHTKIQEDEELKQSIQALLTTKIAVYLMTFLIVTVAWAAHIRLFQVIERIDDTLALLNLACMMLITFLPYTFSLMATFPNNILGILLFCACVMVIGLIQALIVLYGFSHPFLLNDQIQMSENQAYYKQHILKVIMRVPIMCLFASIFSFIFFQLSYVLLAIVIFLPYISQCLKWIRSKAIGGQTDESPDSMPFYTYHPSEPLSKERVEAFSDGVFAIVATLLILDICEGNVPDPSVVKKKFDNSLIAALQEYGPEYLAYFGSFVTVGLLWFVHHSLFLHVTKATRLMGLFNTFSLAFVGGLPLAYQLTHESPRGSRNELEAVQISCVIIFFASLFQLAIWVTALFTERETLHPYVRYGGREHTFMLAKLSLYPCVALGTFFITCILSRFSAPIFHMMEICIPFAFLLLRLLVRVALALLRWLFCSARNDLERIPVEEEESRLPINDIVT",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should contain Helical; Name=TM4-1, Helical; Name=TM6-2, Helical; Name=TM2-1, Helical; Name=TM5-1, Helical; Name=TM5-2, Helical; Name=TM4-2, Helical; Name=TM2-2, Helical; Name=TM1-1, Helical; Name=TM3-1, Helical; Name=TM6-1 transmembrane regions to facilitate transport across the membrane.2. The protein design must feature the RxxxFSD motif motif.3. The Cytoplasmic, Lumenal topological domain of the protein should be designed to be flexible or rigid.4. The protein should have a fast turnover rate for K(+)(in) = K(+)(out).5. A protein that can perform potassium ion leak channel activity, proton channel activity, arachidonic acid binding in endosome, lysosome, lysosomal membrane, endosome membrane to support neuron cellular homeostasis, proton transmembrane transport, potassium ion transmembrane transport, lysosomal lumen pH elevation, phagosome-lysosome fusion. The designed protein sequence is ",
    "response#1": "LAFQSVGIIYGDIGTSPLYVYSSTFPDGIGHRDDLVGVLSLILYTLIIIPMLKYVFIVLYANDNGDGGTFALYSLISRYAKIRMIPNQQAEDAMVSNYSIEAPSSQLRRAQWVKHKLESSRAAKMALFFLTILGTSMVMGDGTLTPAISVLSAVSGIREKAPNLTQTQVVLISVAILFMLFSVQRFGTDKVGYTFAPIISVWFLLIAGIGLYNLVVHEITILKAFNPWYIVQYFRRNGKKGWVSLGGVVLCVTGTEGMFADLGHFNIRAVQISFNCILFPSVALCYIGQAAYLRKFPENVSDTFYKSIPGKYRDRLNFGPLFWPTFIVAILAAIIASQAMLSGAFAILSKALSLGCLPRVRVIHTSKKYEGQVYIPEVNFMMGLASIIVTIAFRTTTSIGNAYGICVVTTFMVTTHLMTVVMLLIWKKHLVFILLFYCVFGFTEVVYLSSILSKFVDGGYLPFCFAMVLMTMMATWHYVHVRRYWYELDHIVPTAEGVALFYTELVQGIPPIFPHLIEKIPTIHSVLVFISIKHLPVPHVDTSERFLFRQVELKDYKVFRCVARYGYRDSLEEAKDFVVTLLENLQDYIRDVNLYTDEPHTISAHSSCNHSFSREKPSGRYAVHAEDMLTPIESFSEITALSNYGSDRLPHFKASKMNMEELAKIEQEQMFIEKEMEKGVVYILGETEVVVRPHSSLLKKIVPGLCFFYSNRQDGGWLTPVLAHYIRNMRSLHEVTVFLTLRYLLVAKVDGKDRVQAVRRLGPAGVYGCTIQYGYADAIDFEEDDIAGQVVGALRERVVDGEEEGERVEAARAAGVVHVRGKMRFHVGKDTRLFDRVLLGFYELLHGACRSALPALGIPLQQRVEIGML",
    "response#2": "PGVGLLYTELVQGIPPLFPRLVRKIPSVHAVFVFISIKHLPIPHVAAAERFLFRQVGPRARRVFRCVARYGYTDALEEPREFAAFLVDGLKMFIQEESAFAPHQEMIDAAADDDDEAAARPRRSTSSAVHSEEAIQAASSGRTTASSVQLQAGGEPPAAMDVEEEKRLIDREVGRGVVYLMGEANVSAGPNSSILKRIPGVGLLYTELVQGIPPLFPRLVRKIPSVHAVFVFISIKHLPIPHVAAAERFLFRQVGPRARRVFRCVARYGYTDALEEPREFAAFLVDGLKMFIQEESAFAPHQEMIDAAADDDDEAAARPRRSTSSAVHSEEAIQAASSGRTTASSVQLQAGGEPPAAMDVEEEKRLIDREVGRGVVYLMGEANVSAGPNSSILKRIL",
    "response#3": "LIIVLSLVGFWSAISSPIYFPALPTLTKYFNTTPSVMNISVVAYLIFQGIAPTISSNLADTFGRRPVILGSIIVFCAVCIAISQTNVYWLLALLRCFQAAGIAPVFAISSGVAGDICTPANRGGMVGAVSGLQLAGNGIGGLVGAALISGFHTWRAIFIFLAIGGGVTFIFAFLVLAETSRRIVGNGSIRPKNVLNKAVLIYLPHFKNKITNDYSTLQPKGPFDILGPFKIFFQKEVFCTLLPSGMHFAAWTVSLTSLSTELESAKYNYSVMKVGLVYLPQGIACFIGSLIAGRCLNWYYRYRKNLYDKQMNDVPLNDRPPFNLVASRLTLTIVPLAMMVIGLSAFGWCLEYKKPIISIIISTILISFSASVMMSICTTMLVDLYPKQSGASASCVNLMRCWLAALFTGVLDKIISALGLGGTYTLLTGICLLTDLGLVYVLYTAAYKTLGVVFGGLVTSPLYVYPSMNLTNPTEEDYLGIYSIMFWTLTLIGVVKYICIALNADDHGEGGTFAMYSLLCQHANIGILPSKKIYTEEENLISNQPVVAGRPGRLRRFIESSIIARRLLLLTAILGMCMLIGDGILTPAISVLSAIDGLRGPFPSVSKPAVEGLSAAILVGLFLLQKYGTSKVSFMFSPIMAAWTFATPVIGVYSIWRYYPGIFKAMSPHYIVRFFMTNQTRGWQLLGGTVLCITGAEAMFADLGHFSKRSIQIAFMSSIYPSLVLTYAGQTAYLINNVDDFSDGFYKFVPRPVYWPMFIIATLAAIVASQSLISATFSVIKQSVVLDYFPRVKVVHTSKDKEGEVYSPETNYMLMLLCVGVILGFGDGKDIGNAFGVVVILVMLITTILLTLVMLIIWGTHVVLVALYLVPFLLLEATYVSAVCTKILRGGWVPFAVSVALAAVMFGWYYGRQRKTEYEAANKVTLER"
  },
  {
    "reference": "MRYARVILARKFGMCYGVRRAIDIVVRSLKMGEVVYTLGPLVHNTSVIDWLSSMGVLMERDVTSIDRGTLVIPSHGVGPEVLEACKRQGLKTLDATCPNVRRTQQLVERLTRSGYQVVIVGDPDHTEVKGLAGYAGGKALVVSSAGDVRGADISPRAGVVAQSTLPLGVFCEVVEALKARAPEIIVNNTICAATSKRQEAARELARQADLVLVIGSETSANARRLKEIVEEEGTRAELVENARACELVDFSGVKTLGVTAGASTPSWVIEEVLARMSAFGEENEGAVGMADVERELHEGDLVRGTVVKVGQDEVLVDIGYKSEGVIPLSELSTAKLESASEAVSVGDEIDVEVLSLEDKDGNIVLSKKRADIEKAWERIMNAMATEEPIEGKVTDVVKGGVVVDVGVRGFVPASHIGLRPVRDLHPLIGTTIRMKVLEAEKDRNNVVLSERAVLEREAQEARKRALESLKEGEVREGIVRRVVNFGAFVDIGDGLEGLLHVSDMTWGRTRDPKAIVSEGEKVRVKVLSVDRERERISLGLKQTMPDPWQNIESRYPIGSIVTGKVTKLVDFGAFVELEDGVEGLVHISQLSDRRVAKPDEVVRPGQEVTVKVISVKSADRRIGLSIKEAQPEKEKEDYRRYASRGTEPAGPTLGDVFGDLFEREAREGKEDK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.2. The protein contains novel S1 motif domains that confer a unique function or activity.3. Design the protein with a signal peptide incorporated.4. For general function, the protein need meet that Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.5. The designed protein should have a binding site that is specific to the target [4Fe-4S] cluster, substrate ligand.6. Ideal protein should have a binding affinity for key enzymes in the Isoprenoid biosynthesis pathway to enhance the overall efficiency of the pathway.7. The designed protein should have 4 iron, 4 sulfur cluster binding, nucleic acid binding within ribosome to regulate dimethylallyl diphosphate biosynthetic process, terpenoid biosynthetic process, isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway.8. The protein's Proton donor must be carefully engineered to ensure that the amino acids involved in dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]-   [ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+)   + 2 reduced [2Fe-2S]-[ferredoxin] are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "MIAIDGPAASGKSSTARRLAQRLGYIYLDTGAMYRACALEARKKAIALEDIPAISAMMDTLDITIAQSPHGNILMLHGKDITDAIRSEEVSNLASAISAIPIVREKMVSLQRKLGRDKAVVLDGRDIGTVVFPQAELKIFMIADVEERAKRRYRELQDKGVIASYDKVLKELQERDFNDSNRSIAPLKPAADSISIDTSHMGLEEQVELLYKRLETTPHQKS",
    "response#2": "MIIAIDGPAASGKSSTARRLAQRLGYIYLDTGAMYRACALEARKKAIALEDIPAISAMMDTLDITIAQSPHGNILMLHGKDITDAIRSEEVSNLASAISAIPIVREKMVSLQRKLGRDKAVVLDGRDIGTVVFPQAELKIFMIADVEERAKRRYRELQDKGVIASYDKVLKELQERDFNDSNRSIAPLKPAADSISIDTSHMGLEEQVELLYKHAHSIKTAQTIAIDGPAASGKSSTARRLAQRLGYIYLDTGAMYRACALEARKKAIALEDIPAISAMMDTLDITIAQSPHGNILMLHGKDITDAIRSEEVSNLASAISAIPIVREKMVSLQRKLGRDKAVVLDGRDIGTVVFPQAELKIFMIADVEERAKRRYRELQDKGVIASYDKVLKELQERDFNDSNRSIAPLKPAADSISIDTSHMGLEEQVELLYKQAQSSIAKT",
    "response#3": "MLKIGKLLRGKIESIDDKNLVLNVDENLEVILPIAEISLDERNKFGDTFKVGEYIDGKIISLSNSDGKVVLSRVENKTTLNVVVKEDVKGGIIASYKGIRIFIPASHVELFHVDNLKEYIGKSFDVAIIEYSTKKRQTKIVASRPSSEGLFQMSSEFELFKKSFNNSERLSVIVKEDVKGGVIVEFNSIKLFVPASHLDFIHIENLNEFVGRELEVKVIEMKEEKNQFKIIGSRPSSEGLFQMSSEFELFKKSFNNNERISVVVKEDVKGGVIVEFNSIKLFVPASHLDFVHIENLNEFVGRELEVKVIEMKEEKNQFKIIGSRPSSEGLFQMSSEFELFKKSFSERLSVIVKEDVKGGVIVEFNSIKLFVPASHLDFIHIENLNEFVGRELEVKVIEMKEEKNQFKIIGSRPSSEGLFQMSSEFELFKKSF"
  },
  {
    "reference": "MFLKDILYKVAIEAVKGSTDIAIHNIDFDSRKIVQNDVFIAIRGTVSDGHEYIEKAISLGAIAIVCDTFPENCKEGITYIQVRDTNKALAFIAANYYGNPSQNLKLIGITGTNGKTTVASLLFQLFKKAGFKVGLLSTVKIIVDDIEYKATHTTPDSITINKYLKQMNGAGVEYCFMEVSSHGVHQKRTEALHFEGGVFTNLSHDHLDYHPTFADYRDVKKSFFDNLSKSAFALSNIDDKNGLVMLQNTNARKVTYALKTYANYRAQILENQLSGLLLKINDNEVWVRLIGTFNAYNLLAIYGTAIELGLDHFEVLRLLSELESVSGRFQFIVSNQNITVIVDYAHTPDALENVLKTINDIRTKNEQLITVVGCGGDRDKTKRPIMANIASNLSDKVIITSDNPRTENPEAIIQEMEKGVEPQNYKKTISIIDRKQAIKAACQLAQPNDIILIAGKGHENYQEIQGVRHHFDDMEIVKELLNNLQK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The presence of Mur ligase central, Mur ligase C-terminal, Mur ligase N-terminal catalytic domains is critical for the interaction of the designed protein with its intended targets.2. Incorporating a Meso-diaminopimelate recognition motif motif is critical for the successful design of the protein.3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.4. Ideal protein should have a binding affinity for key enzymes in the Cell wall biogenesis, peptidoglycan biosynthesis pathway to enhance the overall efficiency of the pathway.5. The protein should be able to bind UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate, ATP ligand in a variety of conditions6. The designed protein should be involved in cell cycle, regulation of cell shape, cell division, peptidoglycan biosynthetic process, cell wall organization by localizing to cytoplasm and exhibiting magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity. The designed protein sequence is ",
    "response#1": "MKLDQLLKTVPLKQEYGNVNEITGITADSRKVKPGFLFAALKGVASDGRDYIESAVKAGAVAVLTDDRVPSGITDIYVPDTLRVAAMLANGFFGHPAEKLCIIGVTGTNGKTTTTHLIEKILDDHGFKVGLIGTVTGTNGKTTTTHLIEKILDDHGHLAGLIGTIKMKIGNEVFDVKNTTPDALELQKSFAKMLDVGSDYAIMEVSSHALDIGRVRGVQYHIAVFTNLTQDHLDFHETMEKYREAKGLLFSQMGNTYDSNVKKNKYAVLNADDRASEYYKRITAAQVITYGIDKEADVRATKIVISSHGTSFILESFRGTIEINLKMIGKFSVYNALAATAVAGLCYGIDVEKIKTALEKVPGVKGRMQRLGGGKKPLLVVDYAHTPDALENALSTLREVLPASRRLFCVFGCGGDRDKGKRPQMGRIACHLADTVIITNDNPRKEDPKAIIRDILAGVDGVAAKHINRGDYSILSNRAAAIRSAIELAEPGDIVLIAGKGHETYQIIGTTKHHFDDREVAREALKERG",
    "response#2": "MKLSQLLAPLLPAKHDQSEITGITADSRKVKPGFLFAALKGVASDGRDYIESAVKAGAVAVLTDDRGVADALGALASAWFGRPESDLCLIGVTGTNGKTTTTHLIEKILDDHGCKVGLVGTLYTRITGTNGKTTTATLLYELFKKSGYKTGLLSTVCNYIHNEEIPATHTTPDPVQLNALLARMVETGCQYCFMEVSSHSIHQHRIAGLKFAGGIFSNITHDHLDYHKTFDEYIKAKKMFFDNLPGEAFALSNFDDRNGKVMLQNTNAKKYSYSLRSMADFKCRILENHFEGMKLNMDGNEIWTRFIGKFNAYNLLAVYSVACVLGADKAEVLRKLSEPAAVAGRLERITRDEEFSVFVDYAHTDDALAQVLETLREVTPGRLLLVFGCGGSRDTGKRAKMGAVAARLADETIITSDNPRREPPAAIAAEIEQGYRAIREDGYQVELDRQRAIDAIIERAGAGDTVLIAGKGHETYQIIGGRVLHFDDREEALRALG",
    "response#3": "MNLKKLLKDLNLIEESSDMEITGITADSRKVKPGFLFAALKGVASDGRDYIESAVKAGAVAVLTDDREGHIESLAIDSRLVATGSLFLACSGSARHGLEFLSQALSAGAVAVVCEPDEQWSAEHIEQLAAETQVPLLQVEGLSRQVSRIAGRFYGNPSEKMTIIGVTGTNGKTTTTHLIEKILDDHKKIGIIGTIANYIGNNKLEAKNTTPESFELQKYFNKMIDEDIEYVVMEVSAHALELERVYGCSFKAAVFTNLTEDHLDFHGSMESYFEAKKKLFSMLNPEAVVNLDNDDAEKYIKEADCEVYSFGINQKADLKASNIKLSKAGSSFELVSGNEIYSISLKMVGKFNVYNALAAISACFSLGISLEDIKRAIESIKGIPGRLELVGQTALKAPVFVDYAHTPDGLNVLLRAARPHVRGKLIVVFGCGGDRDATKRSIMGDVARRLADEVIITDDNPRSEEPAKIRAAIRNGAPDASEIGDRATAINEGIRRLKAGDALLIAGKGHETYQIIGGKTLDFDDRLVAEEAIKGRK"
  },
  {
    "reference": "MQTAMRTDVIVVAAGSGQRAGEGMPKQYRHIGGQTVLRRTLERFATHEGIRRVLPVIHPDHAALCAESARELPGILTPVAGGATRQASVRAGLEALADDPPDLVLIHDAARPFVSARLISDAIATAQRHGAAVPALALVDTIKAVPEPGFLAGGPSRDGLRSVQTPQSFRFDLILAAHRGQIGRDDLTDDAAVAEAAGHPVASFAGDPANVKLTTPEDFVTAERNAAAELMDVRTSTGFDVHAFAPGDHVMLGGVRVPHEFGLTGHSDADVVLHAMTDALLGTIGSADIGHHFPPSDPQWKGAASDQFLAHAAALVRAAGGRIAHLDTTVICEAPKIGPHREAMRERIAAIVGISTARVSVKATTSERLGFTGRREGIAALAAATVRLPWID",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should take into account the physiological conditions of the Isoprenoid biosynthesis, isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6, isopentenyl diphosphate biosynthesis via DXP pathway pathway.2. For general function, the protein need meet that Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D- erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C- methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).3. The designed protein should have 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity, metal ion binding, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity to facilitate terpenoid biosynthetic process, isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway. The designed protein sequence is ",
    "response#1": "MADERLSRQEQRSPLPDVRTGNGYDVHQLVPGDGVTLCGVFIPHDQKLSGHSDADVALHALTDALLATCGAGDIGDHFPPSDMQWKGAPSRIFLEHAARIVREHGGTIMNADVSLIAEAPKVGPHRETMRRNLSEFLDIDMERCSVKATTNETIGFVGRREGIAAIATATVELPAAEDAHAQLRTGFGTDVHAWAPEDAPRPLWLGCVEWPGQRGVAATSDGDVAAHAACNALLSAAGLGDLGAVFGVDRVEMRGATGAAMLEHVAGLLAAAGFRIGNVAVQVVAPRPKVGTRRAEMERAMSAALGGAPVSVSAATTDRLGFIGRGEGLVGFATALVELLA",
    "response#2": "MADERLSRQEQRSPLPDVRTGNGYDVHQLVPGDGVTLCGVFIPHDQKLSGHSDADVALHALTDALLATCGAGDIGDHFPPSDMQWKGAPSRIFLEHAARIVREHGGTIMNADVSLIAEAPKVGPHRETMRRNLSEFLDIDMERCSVKATTNETIGFVGRREGIAAIATATVGSDGDAVAHAIVDALLGPAAEDLRVVASDADVLAHAVGDAVLPRTGFGTDVHAWAPEDAPRPLWLGCVEWPGQRGVAATSDGDVAAHAACNALLSAAGLGDLGAVFGVDRVEMRGATGAAMLEHVAGLLAAAGFRIGNVAVQVVAPRPKVGTRRAEMERAMSAALGGAPVSVSAATTDRLGFIGRGEGLVGFATALVVA",
    "response#3": "MADERLSRQEQRSPLPDVRTGNGYDVHQLVPGDGVTLCGVFIPHDQKLSGHSDADVALHALTDALLATCGAGDIGDHFPPSDMQWKGAPSRIFLEHAARIVREHGGTIMNADVSLIAEAPKVGPHRETMRRNLSEFLDIDMERCSVKATTNETIGFVGRREGIAAIATATVFERAREVAALTPRVGTGYDVHRLVAGKPLWLCGVEIAHERGLSGHSDADVAIHALVDALLGALAEGDIGSHFPPSDPQWKGAPSHTFLTFARDRVAARSGEIAHVDVSIICEAPKIGPHREAMRARLAELLEIDPGRVSVKATTTERLGFTGRREGIAAQAVATILR"
  },
  {
    "reference": "MAKRRKTSREKKEDTNWQERVIQIRRVSKVVKGGKKLSFRAIVVVGNETGQVGVGVGKAGDVIGAVRKGVADGKKQLIEVPLTKSNSITHITNGVSGGAKVVVRPAAPGTGVIAGGAVRTVLELAGVKNILAKQLGSNNPLNNARAAINALETLRTFSEVAEERGVSVEHLYT",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body.2. The protein must have high specificity towards rRNA binding, structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "MF",
    "response#2": "MRYIGPVCRLCRREGVKLYLKGERCYSPKCAMERRPYPPGQHGQKRARRPSDYAVRLREKQKLRRIYGISERQFRNLFEEASKKKGVTGSVFLGLLG",
    "response#3": "MKILNEWF"
  },
  {
    "reference": "MLTSKKIIVCVTGGIAVYKAVALVSKLTQAGANVKVIMTKSAMEFVQPLTFQAMSRNDVYFDTFDEKDSSVIAHINLADWADLVIVAPATANVIGKLANGIADDMVTTTLLATTAKVWIAPAMNVHMYENAAVIRNIDQLSKDGYSFVEPSEGFLACGYVGKGRLEEPEKIVQLVEDYFSPKDLLLKGKKVVVTAGPTRERIDPVRFLTNFSSGKMGYAMAEAAASLGAETILISGPVCINPPSNVTLISVESAKEMYEAVIEQFETASIVIKTAAVADYKPKEIHDQKMKKQPGEATIVLERTTDILAAIGQRKTTQLLIGFAAETNDVTHYAKGKLAKKNADYIIANDVTEEGSGFGTDTNSVTLVGKNDVEIHFDHLPKKELALQLLQTIMKLETAESK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design must be able to perform phosphopantothenate--cysteine ligase activity in order to contribute to pantothenate catabolic process.2. We need a protein that can perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2 with high efficiency.3. The designed protein should be able to compete with endogenous proteins or inhibitors for CTP binding.4. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal domain.5. We require a protein that can specifically bind to the metabolites produced in the Cofactor biosynthesis pathway.6. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine. The designed protein sequence is ",
    "response#1": "KRVVLGVTGSIAAYKAAELASSLAALKADVHVAMTPSAVHFVGPATFRALTGHTPLTGVFDEIEERTVAHVALVENADLLVIAPATGNIIGKIANGIADEIVSTLAMAAPCPVLLAPAMNTHMWTNPIVIANVDRLRNMGYHFVDPVEGRLACGYEGVGKMAPVERILEAVELAGVRVVVSGGGTHEHLDPVRYLGNSSSGQQGVAIARAARMRGADVTLVAANLQVPVPAAVDVVSVSSTADLDEAMRRHADADVLVMAAAPADFTPASPSGSKIKKDTDSGLTVDLVQTTDVLAGLARNRVPGQLIVGFAAETAADSEDLIRLGRQKLARKGADLLVCNDVSGGAVFGDAANSVVIEHGDRVLEGRRVVVSAGGTREPLDPVRFLGNRSSGRQGYALARVAAQRGASVTLVAAHTADLGDPAAVEVVRVGSAAQLREAMHATVKGADAVVMAAAVADFRPAALAQSKIKKSGAEPDPLALAKNPDILVELVKAREPGQVIVGFAAETGDASGDALHHGREKLARKGCDLLVVNVVGDGQAFEVPDNAGWVLDAAGEVVALEGRRVVVSAGGTREPLDPVRFLGNRSSGRQGYALARVAAQRGASVTLVAAHTADLGDPAAVEVVRVGSAAQLREAMHATVKGADAVVMAAAVADFRPAALAQSKIKKSGAEPDPLALAKNPDILVELVKAREPGQVIVGFAAETGDASGDALHHGREKLARKGCDLLVVNVVGDGQAFEVPDNAGWVLGADGSEVARA",
    "response#2": "KHILLGLTGGVACYKSAELCRSLIKHGATVQVVMTNSATQFITPVTMQALSGNTVYTDQWDARIHNNMAHIELSRAADAILIAPCSTNFIAKLAQGFCDDLLSTLCVARPASVPLFIAPAMNVEMWQKPATQRNVATLREDGLGIFGPAPGEQACGEVGMGRMLEPEQLISELLAALQVQQQDLWAGKNVLISAGGTREYLDPVRFLGNRSTGEMGVQIAAAAARRGASVTLVAAHLEVTPPSGVRVIEAPDVMALAEVMIRESVEADVVFMTAAVADWVPDKFSAEKLSKADLGAHWAPELVPAPDILAQLGAKKPWGQILVGFTAETEVDEQIRADRAKDKMAAKNVDVMVANLVGERVGFGAVETTVTAMFADGSRVTLPVQPKREVADAILDLIVDRLRRESQ",
    "response#3": "KKVLLGISGGIAAYKTTFLVRLLIKAGADVKVVLTESASSFVTPLTLATLSKNPVLTSFVDQEEGSLDWNNHVALGLWADIMLIAPATANTLSKMANGTCDNLLMATYLSAKCPVYFAPAMDLDMYQHPSTAASFEKLASFGNIMIPATSGELASGLYGEGRMAEPEEIVDFLQEELLSTSFPWAGKNVLISAGGTREYLDPVRFLGNRSTGEMGVQIAAAAARRGASVTLVAAHLEVTPPSGVRVIEAPDVMALAEVMIRESVEADVVFMTAAVADWVPDKFSAEKLSKADLGAHWAPELVPAPDILAQLGAKKPWGQILVGFTAETEVDEQIRADRAKDKMAAKNVDVMVANLVGERVGFGAVETTVTFLFPDGSREELPLMGKQAVAEKVLDLIVKRLEEKA"
  },
  {
    "reference": "MAPQQKNRVFVIGVGMTKFEKPGSRKDFDYPDMAKEAGEKALADAGIPYSAIEQACVGYVYGDSTCGQRAIYHSLGLTGIPIINVNNNCSTGSTALFMCRQLIQGGLADCVLALGFERMERGSLSAKFTDRVNPVDKHMEVMVNRHGWAAAPPAPQMFGNAGREHMEKYGTKLEHFAKIAWKNHKHSMNNPYSQFQDEYSLEQVMNSKKVFDFLTLLQCCPTSDGAGAAVLASEAFVRKHHLENQAVEIVAQEMVTDLASTFNENSTIKMVGYDMTRLAAAKCFEATGLKPSDVDVIELHDCFSANELITYEALGLCEEGKAGELIDRGDNTYGGKFVVNPSGGLISKGHPLGATGLAQCAELCWQLRGLAGKRQVPGSKLGLQHNIGIGGAVVVTLYKMGFPKESSSGTGLEGFKSYSIFKEIEKRLQEEGEQLVKKVGGVFAFKVKDGPNGSEATWVVDVKNAKGSVTNDPDKKADCTLSLSDEDLRDLMMGKLTPRVAFFNGKLKVSGNMGLAMKLQNLQVTPGRAKL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must possess acetyl-CoA C-myristoyltransferase activity, acetyl-CoA C-acyltransferase activity to support lipid transport under certain conditions. The designed protein sequence is ",
    "response#1": "GDDSAVEILKKNLEKEINKVYKNLSEFQRLQLARHPDRPYSLDYVRALLKNYYEIHGDRVFRDDPSIVCFLGYIADKKIVVIAEQKGRGTKYKLKRNFGMPHPEGYRKALRVAKLAEKFSIPIIFFIDTPGAYPGIGAEERGQSEAIARNLFEFSDIKTPTIAIVIGEGGSGGALAIGVADKLAMLSNSIFSVISPEGCAAILWKDPDKSEAATKALKITAEELKELNLIDDVIKEPKMGAHRDKDAAAKAVGNYIIEKLNS",
    "response#2": "GDDSAVEILKKNLEKEINKVYKNLSEFQRLQLARHPDRPYSLDYVRALLKNYYEIHGDRVFRDDPSIVCFLGYIADKKIVVIAEQKGRGTKYKLKRNFGMPHPEGYRKALRVAKLAEKFSIPIIFFIDTPGAYPGIGAEERGQSEAIARNLFEFSDIKTPTIAIVIGEGGSGGALAIGVADKLAMLSNSIFSVISPEGCAAILWKDPDKSEAATKALKITAEELKELNLIDDVIKEPKMGAHRDKDAAAKAVGNYIIEKLNG",
    "response#3": "GDDSAVEILKKNLEKEINKVYKNLSEFQRLQLARHPDRPYSLDYVRALLKNYYEIHGDRVFRDDPSIVCFLGYIADKKIVVIAEQKGRGTKYKLKRNFGMPHPEGYRKALRVAKLAEKFSIPIIFFIDTPGAYPGIGAEERGQSEAIARNLFEFSDIKTPTIAIVIGEGGSGGALAIGVADKLAMLSNSIFSVISPEGCAAILWKDPDKSEAATKALKITAEELKELNLIDDVIKEPKMGAHRDKDAAAKAVGNYIIEKLNG"
  },
  {
    "reference": "MKRIGFVGWRGMVGSVLMQRMREEGDFADIEPVFFTTSQAGQPGPDVGKDVPPLKDARDIAALAEMEAIITCQGGDYTEAVYADLRKSGWDGYWIDAASTLRMADDSVIVLDPVNKDVIRNALSAGTKAYVGGNCTVSLMLMAIGGLFQHDLVEWISPMTYQAASGAGAQNMRELVAQMGYLHQVGEKLLADPSSAILDLDRLISGAMTGQDYPAEHFGVPLAGSLIPWIDKKLESGQSREEWKAGVETNKILGRGSNPVPIDGLCVRIGAMRSHAQALTIKLKKDAPLDELTQMLDEANDWVSVVPNEPEASKRDLTPAAVTGTLKTPVGRLRKLAMGPEYLSAFTVGDQLLWGAAEPLRRMLRILQEG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a high specificity for phosphate, NADP(+) binding, with minimal off-target effects.2. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.3. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor, Acyl-thioester intermediate to enable effective catalysis of L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.4. The protein design must be able to perform NAD binding, protein dimerization activity, NADP binding in order to contribute to diaminopimelate biosynthetic process, lysine biosynthetic process via diaminopimelate.5. Ideal protein should have a binding affinity for key enzymes in the L-threonine biosynthesis, Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5 pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MKVAIVGASGAVGQEFLRILAERNFPMDELVLFGSERSAGRKYTFKGKDHEVKLLQHNDDFKDIDIAFVSAGGGTSKDFAETITKHGTVMIDNSSAFRMDEDVPLVVPEVNAALKAAPKGITVNCTCVRVPVARSHLAQVLRPLHQEAQLTALVVATYQAVSGSGLSGVAELQGQIDAVGSKASALTHDGAALDFPEPKQYVRPIAFNVLPLAGSIVDDGLNETDEEQKLRSESRKILEIPDLKVSGTCVRVPVFSGHSLQVNARFATPISPERARELLADAPGVELSEIPTPLQAAGQDPSYVGRIRADETAENGLSLFISNDNLRKGAALNAVQIAELVAADLLER",
    "response#2": "MINIALVGATGLVGRTVTKILEERNFPINNLYLFASSKSKGKVIEFKGHENEVEELTEASLLEKELDIAFFTAGAEVSKKYVPLLREKGVVVIDNSSAFRMDEDVPLVVPEVNPEDILTAEDKIIANPNCSTIQMVLSLEPIRQAYGLNNLIVSTYQAVSGSGVSAIEELHEQSVQWEKGKDVEATILPVKGDKRHFPIARNVLPQIDVFTDNGFTYEEMKMINETKKIMHAPELPVAATCVRVPVVAGHSESIFIEVEQAGVTVADIQNVLKAAPGVVLQDDPANQEYPTPLEAEGKGETFVGRIRKDLNNDKGFHLWVVSDNLLKGAAWNSVQIAEILVAKGWVK",
    "response#3": "MAKVAVVGATGLVGSKMLQVLTERDFPVTELIPVASEKSVGKEVTFKGKPYKVVNADTAISMKPDVALFSAGGSTSLEWAPKFAAAGITVIDNSSAWRMDPDKKLVVPEINGKTLTPDDKIVNCTCVRVPVARSHLQPLEKLSHIKRIRVSSYQSASGAGAAAMAELQTQYSQLVKGEEPTVSKFPHQLAYNVIPQVDVFTDNGYTKEEMKMYHETQKIMHTDAKCSATCVRVSSLRSHSESVWIETERPLSIEEARKAIAAAPGCTLKDDPSQGVYPMPLETGGKDDIFVGRIRKDLADENGLTLWLSGDQIRKGAALNALQPLEKLSHIKRIRVSSYQSASGAGAAAMAELQTQYSQLVKGEEPTVSKFPHQLAYNVIPQVDVFTDNGYTKEEMKMYHETQKIMHTDAKCSATCVRVSSLRSHSESVWIETERPLSIEEARKAIAAAPGCTLKDDPSQGVYPMPLETGGKDDIFVGRIRKDLADENGLTLWLSGDQIREGAQKAVELLQPLEKLSHIKRIRVSSYQSASGAGAAAMAELQTQYSQLVKGEEPTVSKFPHQLAYNVIPQVDVFTDNGYTKEEMKMYHETQKIMHTDAKCSATCVRVSSLRSHSESVWIETERPLSIEEARKAIAAAPGCTLKDDPSQGVYPMPLETGGKDDIFVGRIRKDLADENGLTLWLSGDQIREGAQKAVELAKAIENLSHIKRVHVATYQAASGAGAAAMDELYEQYRQVLANEPVTVEKFAYQLAFNLIPQIDVFTDNGYTKEEMKMFNETRKIMHSDIQVSAMCVRVPALRSHSESIWVETERPISPEEARKAFAKGEGLVLMDNPEKKEYPMPLFLAGKDPVYVGRIRKDLANENGLTFWIVGDQ"
  },
  {
    "reference": "MKKLVLLRHGESQWNRENRFTGWVDIDLSEKGREEAKAAGQLLKDEGFVFDMAYASVLKRAIRTLWTVLDQMDLMWIPVTKSWRLNERHYGALQGLNKTETAQRHGDEQVLIWRRSYDTPPPALDADDERHPSKDRRYAALTPEELPATECLKDTVARFLPYWHETIAPQIMDGKRVIITAHGNSLRALVKYLDNISDEDIVGLNIPTGIPLVYELDDDLKPIRSYYLGDQEELKKAQEAVAKQGKA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that is capable of catalyzing reactions within the Carbohydrate degradation, glycolysis pathway with high efficiency and specificity.2. The protein's Tele-phosphohistidine intermediate must be carefully engineered to ensure that the amino acids involved in (2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate are properly positioned for optimal catalysis.3. The designed protein should have 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity to facilitate glycolytic process.4. For general function, the protein need meet that Catalyzes the interconversion of 2-phosphoglycerate and 3- phosphoglycerate.5. The protein should have a high affinity for substrate. The designed protein sequence is ",
    "response#1": "KIVLLVMDGIGDIPNEEGLTPLQKANTPNLDALAKKSDLGQTIPVLPGITPGSGPGHLGLFGYDPLKYQIGRGILEALGINVEVGENDLVARGNFATIDGDIIVDRRAGRPSSEESAKVVEILNENIKEIEDVKITFYPGKEHRFVVKFTGEGLMDKLEDADPQKEGKPIKYTKALDESSKKSERIVNILLDKIKEVLKDQPKMNFALLRGFSKHPDMPKFGDVFKLKPAAVAVYPMYKGLAKLVGMEVVEAGQTIEDEFNTVKKLWNEYDFFYVHIKKTDSYGEDGNFDSKVKVIEEVDKFLPILLELNPDVLIVTGDHSTPCVMKGHSFHPVPLMIYAKNTRRGLSKLFNEFECARGSLGTIHAVDVMPLAELRS",
    "response#2": "KILMVVLDGVGGLPLTVNGETELATAKTPHLDALAAESQLGLVELVAAGITPGSGPGHLSLFGYDPLKYVVGRGALSAVGIGVKLNAGDVAVRGNFASLGESRLIIDRRAGRPSNEKNAEVVAKLRAAIPEIDGTPVEIYSESEHRFVVVFRATGTPLGADVSDVDPQVTGVPPRTAMAHDPASERTAHLVNAFVTRAEAALADESQVNGVLFRGYSDVPHFPNFDEIYQLRAACIASYPMYKGLASLVGMDVLPVEGEEDALAGKVAALKEHWHDYDFFYWHVKKTDSTGEDGDFAAKVKKIELFDALLPELRALQPDVLCIVGDHSTPSKLASHSWHPVPLLINSRYGRKDLAQRYTEEEAQKGSLGLRRGTDVMPLLMD",
    "response#3": "MKILMVVLDGVGGLPLTVNGETELATAKTPHLDALAAESQLGLVELVAAGITPGSGPGHLSLFGYDPLKYVVGRGALSAVGIGVKLNAGDVAVRGNFASLGESRLIIDRRAGRPSNEKNAEVVAKLRAAIPEIDGTPVEIYSESEHRFVVVFRATGTPLGADVSDVDPQVTGVPPRTAMAHDPASERTAHLVNAFVTRAEAALADESQVNGVLFRGYSDVPHFPNFDEIYQLRAACIASYPMYKGLASLVGMDVLPVEGEEDALAGKVAALKEHWHDYDFFYWHVKKTDSTGEDGDFAAKVKKIELFDALLPELRALQPDVLCIVGDHSTPSKLASHSWHPVPLLINSRYGRKDLAQRYTEEEAQKGSLGLRRGTDVMPLLMY"
  },
  {
    "reference": "MRFDILTLFPEFFTSPLQTSLLGKALANEVAEVHLTNPRDFTTDKHHRVDDEVYGGGVGMLMKPEPIFAAVESLPCLPRRQIVLFTPQGRPMDQPLFHELANHYDQLVMICGHYEGVDERVAEHLVTQELSLGDFVLTCGEIPALTLLNGVIRLRPGTVGKVESLKKESFETELLDYPQYTRPAEFRGWQVPSVLRSGNHALIERWRQEQQIQRTRDRRPDLYQKWLKKSDTSDP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be expressed specifically in the cytoplasm2. The protein should be designed to catalyze the following reaction guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-   methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "FSKIGHIIHLNLKNHLMPYRRLIGEVLMDKVADCRTVVNKSNSIQNTYRNFEMELICGVPEYEVSIKENGCTYKFNFSRVYWNPRLSTEHQKITDMLEEGDLLYDLYAGVGPFTVPAAKRGCTVIANDLNPDSYSALVINCGLNKVMRNVKCYNMDAVDFIKVELRNDLLAKLADDKFQGNIHITMNLPAMAVEHLVHFPGLFSGESIELRIKPLVHVYCFAQGADDKKPIAQQKVEQWLGVEVTDMLKEITFVRNVAPNKDMMRVRNPSAC",
    "response#2": "FSKIGHIIHLNLKNHLMPYRRLIGEVLMDKVADCRTVVNKSNSIQNTYRNFEMELICGVPEYEVSIKENGCTYKFNFSRVYWNPRLSTEHQKITDMLEEGDLLYDLYAGVGPFTVPAAKRGCTVIANDLNPDSYSALVINCGLNKVMRNVKCYNMDAVDFIKVELRNDLLAKLADDKFQGNIHITMNLPAMAVEHLVHFPGLFSGESIELRIKPLVHVYCFAQGADDKKPIAQQKVEQWLGVEVTDMLKEITFVRNVAPNKDMMRVRNPSAC",
    "response#3": "FATTGHIAHLNLNDEYLPYKYIIGQIIMDKNLRIRTVVNKLNNIHEQFRFFEMELVAGEPDFVVEHLETDCRFTFDFREVYWNSRLHTEHERLVSQFEPQDGIVADVMAGVGPFAVPAAKKGCAVFANDLNPNCEKWLRKNVTDNHVSVNLRVFCEDGRDFIRASFKRAHGEPFPPYAGKKLSKRQEKEERKRRQDVAPERTNSSGPDIALSDKPIRNRIAHFVMNLPDTAIEFLDAFRGVFKTAERELSGLYEMMPVVHCHCFTRYLDAVDAKVDIRKRVEKALGGELEDATVHFVRLCTVNASPYFIASIMLY"
  },
  {
    "reference": "MEAGDEEHRPPPLSGRYVVLGVTGGIAAYKAAELCRRLVEAGAHVAPVMTDDATRFLGEVTLSALASEPVRRSLWDAPEPIPHTRLGQRAHVVVVAPATARFIGAYAHGISDDLLTATVLATRAPVVVCPAMHSEMWEHPAVQDNLELLRRRGVTVVPPAEGVLAGGDVGPGRLAGPEDILGAVALVLGAQETGPGGDLAGHRVVVTAGGTREAIDPVRFITNRSSGKQGHAVAEAAARRGALVTLVTTTDRPVSPSIGVVRVESAASMEQAVLEAAAHADVVVMAAAVADFRPKAPASVKLTKADGVPDLVLEPTPDILTELARRRRGGQVLVGFAAETHDVRSRARAKLEAKGVDVLVANDVAAPGVGFDHDTNAVTILGADGSCREIGLTSKEAVAEAVLDAVAARLPSRD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Ideal protein should have a binding affinity for key enzymes in the Cofactor biosynthesis pathway to enhance the overall efficiency of the pathway.2. The designed protein should contain one or more DNA/pantothenate metabolism flavoprotein C-terminal domains that are essential for its function. The designed protein sequence is ",
    "response#1": "WQGRRLLVTAGSTVEVLDPARLLINRSSGRMGVLLAQAARLRGAQVDLVHGPLKLPLAWLEGLAAYPVASADEMQNMLAQLQSKADAVAMAAAVADVRRVNPAQAEKLSKDCLLQSFSQGWEQVPDLLAELVLRRPVGQVLLGFAALTGNEAQIQQLGEEKRLRKGCDLLMANPIDRPGQGFEEDFNGGWH",
    "response#2": "WQGRRLLVTAGSTVEVLDPARLLINRSSGRMGVLLAQAARLRGAQVDLVHGPLKLPLAWLEGLAAYPVASADEMQNMLAQLQSKADAVAMAAAVADVRRVNPAQAEKLSKDCLLQSFSQGWEQVPDLLAELVLRRPVGQVLLGFAALTGNEAQIQQLGEEKRLRKGCDLLMANPIDRPGQGFEEDFNGGWQ",
    "response#3": "WQGRRLLVTAGSTVEVLDPARLLINRSSGRMGVLLAQAARLRGAQVDLVHGPLKLPLAWLEGLAAYPVASADEMQNMLAQLQSKADAVAMAAAVADVRRVNPAQAEKLSKDCLLQSFSQGWEQVPDLLAELVLRRPVGQVLLGFAALTGNEAQIQQLGEEKRLRKGCDLLMANPIDRPGQGFEEDFNGGWIG"
  },
  {
    "reference": "MPSTYDIYKKLLLLASFLSASQAQQVGTSKAEVHPSLTWQTCTSGGSCTTVNGKVVVDANWRWVHNVDGYNNCYTGNTWDTTLCPDDETCASNCALEGADYSGTYGVTTSGNSLRLNFVTQASQKNIGSRLYLMEDDSTYKMFKLLNQEFTFDVDVSNLPCGLNGAVYFVSMDADGGMAKYPANKAGAKYGTGYCDSQCPRDLKFINGMANVEGWEPSANDANAGTGNHGSCCAEMDIWEANSISTAYTPHPCDTPGQVMCTGDSCGGTYSSDRYGGTCDPDGCDFNSYRQGNKTFYGPGMTVDTKSKITVVTQFLTNDGTASGTLSEIKRFYVQNGKVIPNSESTWSGVSGNSITTAYCNAQKTLFGDTDVFTKHGGMEGMGAALAEGMVLVLSLWDDHNSNMLWLDSNYPTDKPSTTPGVARGSCDISSGDPKDVEANDANAYVVYSNIKVGPIGSTFSGSTGGGSSSSTTATSKTTTTSATKTTTTTTKTTTTTSASSTSTGGAQHWAQCGGIGWTGPTTCVAPYTCQKQNDYYSQCL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.2. A protein with cellulose binding, cellulose 1,4-beta-cellobiosidase activity is needed to enhance cellulose catabolic process in a specific context.3. The designed protein is the optimization of the Nucleophile, Proton donor to achieve high catalytic efficiency for Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose   and cellotetraose, releasing cellobiose from the non-reducing ends of   the chains..4. The presence of CBM1 domains is critical for the interaction of the designed protein with its intended targets.5. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "PTNDEFFVEAGINASGTNFIEIKAIVNNQSGWPARATNKLKFRYFVDLSELIKAGYSPNQLTLSTNYNQGAKVSGPYVWDSSRNIYYILVDFTGTLIYPGGQDKYKKEVQFRIAAPQNVQWDNSNDYSFQDIKGVSSGSVVKTKYIPLYDEDIKVWGEEPGHATVFAVWINDEDQGLGNTADGYIRTPPNNSPVTDVTSTDLTCNVNGDQAAAKTLEVAAGDKITFEWHHNSRDSSDDIIADSHKGPVLVYMAPTEAGSAGKNWVKIYEDGYNDGTWAVDTLIANKGKHSVTVPDVPAGNYLFRPEIIALHEGNREGGAQLYMECVQFKVTSDGTTQLPEGVSLPGAYTATDEGILFDIYSSFDSYPIPGPAVPTNDEFFVEAGINASGTNFIEIKAIVNNQSGWPARATNKLKFRYFVDLSELIKAGYSPNQLTLSTNYNQGAKVSGPYVWDSSRNIYYILVDFTGTLIYPGGQDKYKKEVQFRIAAPQNVQWDNSNDYSFQDIKGVSSGSVVKTKYIPLYDEDIKVWGEEPGA",
    "response#2": "TPGEEFYVEAAVNAAGPGFVNIKASIINKSGWPARGSDKLSAKYFVDISEAVAKGITLDQITVQSTTNGGAKVSQLLPWDPDNHIYYVNIDFTGINIFPGGINEYKRDVYFTITAPYGEGNWDNTNDFSFQGLEQGFTSKKTEYIPLYDGNVRVWGKVPHATVFAVWINDEDQGLGNTADGYIRTPPNNSPVTDVTSTDLTCNVNGDQAAAKTLEVAAGDKITFEWHHNSRDSSDDIIADSHKGPVLVYMAPTEAGSAGKNWVKIYEDGYNDGTWAVDTLIANKGKHSVTVPDVPAGNYLFRPEIIALHEGNREGGAQLYMECVQFKVTSDGTTQLPEGVSLPGAYTATDEGILFDIYSSFDSYPIPGPAVTGPRVRVNQVAYLPAGPKNATLVTDATARLPWQLRNAQGTTVARGLTVPRGVDASSGQNVHSIDFGSYRGRGTGYTLVADGETGHATVFAVWINDEDQGLGNTADGYIRTPPNNSPVTDVTSTDLTCNVNGDQAAAKTLEVAAGDKITFEWHHNSRDSSDDIIADSHKGPVLVYMAPTEAGSAGKNWVKIYEDGYNDGTWAVDTLIANKGKHSVTVPDVPAGNYLFRPEIIALHEGNREGGAQLYMECVQFKVTSDGTTQLPEGVSLPGAYTATDEGILFDIYSSFDSYPIPGPAVHATVFAVWINDEDQGLGNTADGYIRTPPNNSPVTDVTSTDLTCNVNGDQAAAKTLEVAAGDKITFEWHHNSRDSSDDIIADSHKGPVLVYMAPTEAGSAGKNWVKIYEDGYNDGTWAVDTLIANKGKHSVTVPDVPAGNYLFRPEIIALHEGNREGGAQLYMECVQFKVTSDGTTQLPEGVSLPGAYTATDEGILFDIYSSFDSYPIPGPAV",
    "response#3": "HATVFAVWINDEDQGLGNTADGYIRTPPNNSPVTDVTSTDLTCNVNGDQAAAKTLEVAAGDKITFEWHHNSRDSSDDIIADSHKGPVLVYMAPTEAGSAGKNWVKIYEDGYNDGTWAVDTLIANKGKHSVTVPDVPAGNYLFRPEIIALHEGNREGGAQLYMECVQFKVTSDGTTQLPEGVSLPGAYTATDEGILFDIYSSFNSTKPVGGHATVFAVWINDEDQGLGNTADGYIRTPPNNSPVTDVTSTDLTCNVNGDQAAAKTLEVAAGDKITFEWHHNSRDSSDDIIADSHKGPVLVYMAPTEAGSAGKNWVKIYEDGYNDGTWAVDTLIANKGKHSVTVPDVPAGNYLFRPEIIALHEGNREGGAQLYMECVQFKVTSDGTTQLPEGVSLPGAYTATDEGILFDIYSSFDSYPIPGPAVPTNDEFFVEAGINASGTNFIEIKAIVNNQSGWPARATNKLKFRYFVDLSELIKAGYSPNQLTLSTNYNQGAKVSGPYVWDSSRNIYYILVDFTGTLIYPGGQDKYKKEVQFRIAAPQNVQWDNSNDYSFQDIKGVSSGSVVKTKYIPLYDEDIKVWGEEPP"
  },
  {
    "reference": "MENRWQVMIVWQVDRMKIRTWNSLVKHHMYISKKARGWFYRHHYESRHPKVSSEVHIPLGDARLIIRTYWGLQPGERDWQLGHGVSIEWQQKRYITQIDPDLADQLIHLHYFDCFSESAIREAILGRIVRSRCEYQAGHNKVGSLQYLALKALVKPTRKRPPLPSVAKLTEDRWNKPQKTRGHRGSRPMNGH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have a specific function in viral life cycle and should be localized to plasma membrane, virion component in order to carry out this function efficiently.2. For general function, the protein need meet that In turn, they are directed to the 26S proteasome for degradation; Finally, Vif also contributes to the G2 cell cycle arrest observed in HIV infected cells. The designed protein sequence is ",
    "response#1": "GLLSRIWG",
    "response#2": "GVAMS",
    "response#3": "GFSSF"
  },
  {
    "reference": "MDHLPIFCQLRDRDCLLVGGGDVAERKARLLLDAGARLTVNARDVVPQFAVWAETGRVTLVKGDFNESLLDSCWLVIAATDDEQVNERVSQAAEARRIFCNVVDAPKQASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLEAILPQHLGQVAHYAGQLRARVKKQFATMSERRRFWEKLFVNDRLAQSLANQDPQAISETTEQLLSEDLDHRGEVVLVGAGPGDAGLLTLKGLQQIQQADIVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHCVPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAASGCSAYAGIPLTHRDYAQSVRLVTGHLKTGSELDWHNLAAEKQTLVFYMGLNQAATIQQKLLEHGMEADMPVALVENGSTIKQRVVDGTLAELGELAQQVVSPSLIIVGRVVSLREKLNWFSHH",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein have Tetrapyrrole methylase domains with specific sequence motifs that are crucial for its activity.2. The designed protein should have a binding site that is specific to the target S-adenosyl-L-methionine ligand.3. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.4. The designed protein must be able to perform sirohydrochlorin ferrochelatase activity, NAD binding under specific conditions.5. For general function, the protein need meet that Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1; Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme. The designed protein sequence is ",
    "response#1": "GEVYLVGAGPGDPDLLTLKALRLMYAADVVLYDRLVSPEIMKRVRPDAERIFVGKEAKHHPVPQEQINDLLVRLAREGNKVLRLKGGDPFIFGRGGEEIDRLAAAGLPFQVVPGVTAASGCAAYAGIPLTHRDYSQSVRFVTGHMKNNQPDLDWPVLAKEEQTLVIYMGLITLSVICEQLQAYGMSSAMPVGIVEQGTMPQQRVVVGTLQDIAGKVEAAQLQAPAIIIIGQVVKLEADVILHDRLVGPEVLALARRDAERIEVGKQAGHHHVTQDRIHALMLEHARAGKRVVRLKGGDGFIFGRGGEELEFLQAHGIAFEVVPGITAALACGAYAGIPLTHRDHAQSVRFVTAHTKDSADAIDWASLAAERQTLAVYMGVAGLETLRERLIAHGRAASTPFALIENGSRPEQRVVVGTLDRLPALARAHAVQSPALLVLGEVL",
    "response#2": "GSVALVGAGPGDPGLLTLRALRVLNEADVILHDRLVSDEVLQLARRDAERISVGKHAGGGGHSQAEINAQLVALAQAGKRVVRLKGGDPFVFGRGGEELQALRAHGIGYEVVPGITAAIACAAYAGIPLTHREHAQSLRIVTAHGKDDIDALDWASLARGRQTLAFYMGVAGLDRIRERLTAHGLPAATPFALVENGSRPEQRVVAGMLADLPALARLHQVRAPALLVVGDVAAFVGAGPGDPGLLTLRALRVLNEADVILHDRLVSDEILQLARRDAERISVGKLAGDGGHSQADINALLVKLAQTGKRVVRLKGGDPFVFGRGGEELQALRTHGIDYEVVPGITAAVACGAYAGIPLTHREHAQSLRIVTAHGKEDLDTLDWTSLARGRQTLAFYMGVAGLERIRERLTSHGLAATTPFALVENGSRPEQRVVTGSLADLPALARLHEVRAPALLVVGEVEADVILHDRLVGPEVLALARRDAERIEVGKQAGHHHVTQDRIHALMLEHARAGKRVVRLKGGDGFIFGRGGEELEFLQAHGIAFEVVPGITAALACGAYAGIPLTHRDHAQSVRFVTAHTKDSADAIDWASLAAERQTLAVYMGVAGLETLRERLIAHGRAASTPFALIENGSRPEQRVVVGTLDRLPALARAHAVQSPALLVLGEVLGGGR",
    "response#3": "VGAGPGNPDLLTFRALRLMQQADVVLYDKLVAPELLELVRRDAERIYVGKARANHALPQDDINQLLVDLAKQGKRVLRLKGGDPFTFGRGGEEIATLAEHGIAFEVVPGITSASGAAAYAGIPLTHRDYAQSVTFVTGHKQDGSIDLDWQALTRPHQTVVVYMGVSTAAELCQAFVDNGRAAATPAAAVEWATTERQRAVCGTLATLPGLMASHGIASPALIIVGEVLRREG"
  },
  {
    "reference": "MASNDTPAFANNEIMLKCEGLTRIYREGPQDVTVLDALELDVRAGERVAVVGSSGSGKTTLLNLLGGLDRPSAGEVRIAGQSLAEMGEAALGSFRNRHIGFVYQFHHLLAEFSALENVAMPLLIRGQTRQEAQARAREILAKVGMEARGEHKPGELSGGERQRVAIARALVTDPSLVLMDEPTGNLDQTTAGHILALMDGLAAQSACAFIIVTHDPGLAAHQDRVMRLDQGRLSEDA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.2. For general function, the protein need meet that Responsible for the formation of the LolA-lipoprotein complex in an ATP-dependent manner.; Part of the ABC transporter complex LolCDE involved in the translocation of mature outer membrane-directed lipoproteins, from the inner membrane to the periplasmic chaperone, LolA3. The protein contains novel ABC transporter domains that confer a unique function or activity.4. The protein should be optimized for plasma membrane-specific folding and stability, while maintaining ATP hydrolysis activity, ATP binding. The designed protein sequence is ",
    "response#1": "PGEVVGLIGPSGSGKSSLLHSAGLLERPDAGLVALEGRDCSKLSERARTRIRLGTVGFVYQFHHLLPEFSALENVAMPLTIAGKSRREAEARARELLESLGLGHRLNHQPAQMSGGEQQRVAIARALANRPKLLLADEPTGNLDPATSTAVFQALYQVCREQGVAAVIATHNMELARYMDRVVALKDGA",
    "response#2": "LSDVSFSVPRAVKVAITGESGCGKSTLLNIIGGMEHADSGIVRVLSCDVLTLHEHALTEYRRQFLGLVFQFHHLLRDFTALENVMLPGLIAGKSYREVRARAYELLEKVRVVQRAHHFPAQMSGGERQRTAVARALINDPTLILADEPL",
    "response#3": "PGEVVGLIGPSGSGKSSLLHSAGLLERPDAGLVALEGRDCSKLSERARTRIRLGTVGFVYQFHHLLPEFSALENVAMPLTIAGKSRREAEARARELLESLGLGHRLNHQPAQMSGGEQQRVAIARALANRPKLLLADEPTGNLDPATSTAVFQALYQVCREQGVAAVIATHNMELARYMDRVVALKDGS"
  },
  {
    "reference": "MDSFMLQDSAALNRVREAQEFLDPQNDSQGRSHRSAIISMVKRKERRLVVNLDLLRAHNSDLAAGVLAQPFDFTLAFHHALKQVVKTLPESGDAEKLNDVAYYCAWAGSFGLNACNPRTLSSQHLNSMVSLEGIVTRCSLIRPKVVKSVHYNENKDIFHFREYQDQTMTNGVTTSSAYPQEDEEGNPLTTEYGFSTYRDHQTISIQEMPERAPAGQLPRGVDVILDDDLVDKAKPGDRVQLVGIYRTLGNRNTNHNSALFKTVIVANHLVLLSSKSGGGVATQALTDTDIRNINKISKKKNLLGLLSQSLAPSIYGHDYIKRAILLMLLGGTEKNLENGTHLRGDINILMVGDPSTAKSQLLRFVLNTAPLAVATTGRGSSGVGLTAAVTSDKETGERRLEAGAMVMADRGVVCIDEFDKMSDIDRVAIHEVMEQQTVTIAKAGIHTSLNARCSVVAAANPIFGQYDTHKDPHKNIALPDSLLSRFDLLFVVTDDIEDARDRQVSEHVLRMHRYRQPGTEDGAPVRENPNQTLGIGTNSQANTPSTADVYEKFDKMLHAGVTLTPGRGGNKKPEVLSIPFMKKYIQYAKNRIHPKLTQLASDRIADIYVGLRNDEMEGNQRRTSPLTVRTLETLIRLATAHARSRLSQQVEERDALAAEAILRFALFKEVDEVESKKKRRKTQATDFASSSEESSSEDDGGDGSWRGATTRTPATQLPRSTREIARPRTIMNSRDDSNGIDNDNDASAEGKDASLPDRTDSEHVYGAT",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit DNA replication origin binding. The designed protein sequence is ",
    "response#1": "MFNPLYIHAGVGLGKTHLLQAVTWAGNAGGERKVLYLTAEKFMYGFVAALKSQTALAFKEALRGIDVLVIDDLQFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVI",
    "response#2": "LDPRLTFASFVVGRANTLAHAAARQVADGRRGDPVMFNPLYIHAGVGLGKTHLLQAVTWAGNAGGERKVLYLTAEKFMYGFVAALKSQTALAFKEALRGIDVLVIDDLQFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVVH",
    "response#3": "LDPRLTFASFVVGRANTLAHAAARQVADGRRGDPVMFNPLYIHAGVGLGKTHLLQAVTWAGNAGGERKVLYLTAEKFMYGFVAALKSQTALAFKEALRGIDVLVIDDLQFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVVV"
  },
  {
    "reference": "PSSPPAGIEKFKGHYLHSRDYKDARDFTDKRVVVIGIGNSGSDLSVEISQTAKQESSRRRVFLSTRRGAWILNRVGDQGYPIDTIFATRMKMFLQNLLSQSTVSGFVEKQLNARFDHSHYGLKPKHRILDQHPTVNDDLPNRIISGRVLVKPNVQEFTETSAIFEDGTREDIDAVVFATGYSFSFPFLEGCVKVVENQIPLYKFMFPPNLEKPTLAFIGLIQPLGAIMPISELQCRWATRVFKGLNKLPPRHDMEADIKQKREAMAKRYVKSQRHTIQVDYIPYMDELACQVGVKPNLLALFLNAPK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The presence of FAD is crucial for the protein to function optimally under specific conditions.2. The protein should have NADP binding, flavin adenine dinucleotide binding and be active in endoplasmic reticulum membrane. The designed protein sequence is ",
    "response#1": "PELGEAAQVLLDHWPGRARGFHRVDLPGFDAVLDLAVMDVVEALEAWDGLADAWFLDGFSPALNPAMWRDEVLAAVGARSAPGARAATFTVAGAVRRGLSAAGFEIAKRPGFGRKRERLEAWL",
    "response#2": "PELGEAAQVLLDHWPGRARGFHRVDLPGFDAVLDLAVMDVVEALEAWDGLADAWFLDGFSPALNPAMWRDEVLAAVGARSAPGARAATFTVAGAVRRGLSAAGFEIAKRPGFGRKRERLEAWL",
    "response#3": "PELGEAAQVLLDHWPGRARGFHRVDLPGFDAVLDLAVMDVVEALEAWDGLADAWFLDGFSPALNPAMWRDEVLAAVGARSAPGARAATFTVAGAVRRGLSAAGFEIAKRPGFGRKRERLEAWL"
  },
  {
    "reference": "VPPKGVSM",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must be able to regulate defense response, vasodilation. The designed protein sequence is ",
    "response#1": "MVFLQGVAGIGKSLMLTKLMLAWSEGMVFQNKFSYIFYFCCQDVKKMKRASLAELISKEWPNASAPIEEILSQPEKLLFVIDNLEVMECDMSEWESELCDDCMEKQPVNILMSSLLRRKMLPESSFLVSATPETFEKIEDRIECTNVKMMAGFNESSIKVYFYSLFQC",
    "response#2": "MVFLQGVAGIGKSLMLTKLMLAWSEGMVFQNKFSYIFYFCCQDVKKMKRASLAELISKEWPNASAPIEEILSQPEKLLFVIDNLEVMECDMSEWESELCDDCMEKQPVNILMSSLLRRKMLPESSFLVSATPETFEKIEDRIECTNVKMMAGFNESSIKVYFYSLFQR",
    "response#3": "MVFLQGVAGIGKSLMLTKLMLAWSEGMVFQNKFSYIFYFCCQDVKKMKRASLAELISKEWPNASAPIEEILSQPEKLLFVIDNLEVMECDMSEWESELCDDCMEKQPVNILMSSLLRRKMLPESSFLVSATPETFEKIEDRIECTNVKMMAGFNESSIKVYFYSLFQF"
  },
  {
    "reference": "MAAYLQWRRFVFFDKELVKEPLSNDGAAPGATPASGSAASKFLCLPPGITVCDSGRGSLVFGDMEGQIWFLPRSLQLTGFQAYKLRVTHLYQLKQHNILASVGEDEEGINPLVKIWNLEKRDGGNPLCTRIFPAIPGTEPTVVSCLTVHENLNFMAIGFTDGSVTLNKGDITRDRHSKTQILHKGNYPVTGLAFRQAGKTTHLFVVTTENVQSYIVSGKDYPRVELDTHGCGLRCSALSDPSQDLQFIVAGDECVYLYQPDERGPCFAFEGHKLIAHWFRGYLIIVSRDRKVSPKSEFTSRDSQSSDKQILNIYDLCNKFIAYSTVFEDVVDVLAEWGSLYVLTRDGRVHALQEKDTQTKLEMLFKKNLFEMAINLAKSQHLDSDGLAQIFMQYGDHLYSKGNHDGAVQQYIRTIGKLEPSYVIRKFLDAQRIHNLTAYLQTLHRQSLANADHTTLLLNCYTKLKDSSKLEEFIKKKSESEVHFDVETAIKVLRQAGYYSHALYLAENHAHHEWYLKIQLEDIKNYQEALRYIGKLPFEQAESNMKRYGKILMHHIPEQTTQLLKGLCTDYRPSLEGRSDREAPGCRANSEEFIPIFANNPRELKAFLEHMSEVQPDSPQGIYDTLLELRLQNWAHEKDPQVKEKLHAEAISLLKSGRFCDVFDKALVLCQMHDFQDGVLYLYEQGKLFQQIMHYHMQHEQYRQVISVCERHGEQDPSLWEQALSYFARKEEDCKEYVAAVLKHIENKNLMPPLLVVQTLAHNSTATL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways; Required for fusion of endosomes and autophagosomes with lysosomes; Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion; The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations; Involved in cargo transport from early to late endosomes and required for the transition from early to late endosomes.2. A protein that can perform protein-macromolecule adaptor activity, nucleotide binding, metal ion binding, ubiquitin protein ligase activity in endocytic vesicle, clathrin-coated vesicle, HOPS complex to support intracellular protein transport, vacuole organization, regulation of organelle assembly, positive regulation of early endosome to late endosome transport. The designed protein sequence is ",
    "response#1": "RPSLEGRSDREAPGCRANSEEFIPIFANNPRELKAFLEHMSEVQPDSPQGIYDTLLELRLQNWAHEKDPQVKEKLHAEAISLLKSGRFCDVFDKALVLCQMHDFQDGVLYLYEQGKLFQQIMHYHMQHEQYRQVISVCERHGEQDPSLWEQALSYFARKEEDCKSFVKYTANWNPLGDSAFYRKYELYSMDWDLKEELKDCLVAAAPYGGPIALLRNCWRKEKAASVRPVLEIYSASGLPLASLLWKSGPVVALGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAYRFTLSANVGDLKLRRMPEVPGLQSAPSCWTTLCHDRVPHILLAVGPDLYLLDHATCSAVTPAGLAPGVSSFLQMAVSFTYRYLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGNAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPVASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLIQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFVGPIFTKQRVDFTPSERITSLVVSCNQLCMSLGKDTLLRIDLGKASEPNRVELGRKDDAKVHKMFLDHTGSHLLVALSSTEVLYMNRNGQKARPLARWKGQLVESVGWNKAMGNESSTGPILVGTAQGQIFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRGFVIATTRQRLFQFIGRAVEDTEAQGFAGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGSLDCGRPDSLLSEERVWEYPAGVGPGANPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVLRDHFLEKFGPLRHMVKDSSTGHLWAYTERAVFRYH",
    "response#2": "RPSLEGRSDREAPGCRANSEEFIPIFANNPRELKAFLEHMSEVQPDSPQGIYDTLLELRLQNWAHEKDPQVKEKLHAEAISLLKSGRFCDVFDKALVLCQMHDFQDGVLYLYEQGKLFQQIMHYHMQHEQYRQVISVCERHGEQDPSLWEQALSYFARKEEDCKKCSICNSALELPSVHFLCGHSFHQHCFESYSESDADCPTCGYTANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWRKEKAASVRPVLDIYSASGMPLASLLWKSGPVVSLGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLQSAPSCWTVLCQDRVAHILLAVGPDLYLLDHAACSAVTPPGLAPGVSSFLQMAVSFTYRHLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGDAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPAASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLTQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFVYTANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWRKEKAASVRPVLDIYSASGMPLASLLWKSGPVVSLGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLQSAPSCWTVLCQDRVAHILLAVGPDLYLLDHAACSAVTPPGLAPGVSSFLQMAVSFTYRHLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGDAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPAASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLTQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFV",
    "response#3": "YTANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWRKEKAASVRPVLDIYSASGMPLASLLWKSGPVVSLGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLQSAPSCWTVLCQDRVAHILLAVGPDLYLLDHAACSAVTPPGLAPGVSSFLQMAVSFTYRHLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGDAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPAASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLTQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFVVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELDTLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRIQRRLEDELGGRFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLAALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKHEAKKYASRVGPEQKVKALLLVGDVAQAAEVAIEHRNETELSLVLSHCTGATDGAIADKIQRARAEYTANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWRKEKPASARPVLEIYSASGVPLASLLWKSGPVVSLGWSAEEELLCVQEDGVVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLXSAPSCWTTVCQDRVAHILLAVGPDLYLLDHAACSAVTPPGLAPGVSSFLQMAVSFTYRHLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGDAPESIQFVLDEDSYLVPELDGVRVFSRSTHEFLHEVPVASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLPQAVQQCIEAAGHEHWPDMQKSLLRAASFGKCFLDRFPPDSFV"
  },
  {
    "reference": "MDRLLVLVRHGQSEWNLQNLFTGWKDPDLTDLGVSEAKAAGRALKTAGVGFDIGFTSDLLRAQRTMKLLLAEFGQPDLQVTKNVSLNERDYGDLSGLNKAEAAQQWGDEQVHLWRRSYDVPPPGGESLKDTVARVLPYYCQEILPAVLNGKRTLVTAHGNSLRALIMVLDKLTPKTIPGMELATGVPIVYRLKADSTVESKQVLEP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be optimized for 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity activity in order to improve glycolytic process.2. The substrate binding site should be stable and able to withstand changes in environmental conditions.3. The protein with the reaction (2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate, the Tele-phosphohistidine intermediate must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "IDLAIEDGSFFQNEAFQRALDRARQPGRTLHLMGLIGDGGVHSHQRHLLALLELAARAGISRVAVHAFTDGRDTAPTSGIEHLRELLAALDRLRTGFVATVSGRYYAMDRDKRWERTKLAYDAIVCGLGQTARSPLEAIERSYAQGITDEFIVPTVIVDAEGKPLATINDGDAVIFFNFRADRARQLTQALTDAGGFTAFPRCRWPRDLLMVTMAEYH",
    "response#2": "VFWILFDGGGDRPVGGRTPFHTAFKPTIDYLTSLGSCGILDPIAPGVRPGSDTAHLALFGYDPYKYYTGRGAFEALGAGLELKPGDVAFRTNLATIDDNGMVLDRRAGRYITPEEVEAVENLMANIAEDIKRKYGVEIIYKSTVEHRGVLVLRGSVSHKVSDTDPHKVGAKLLESRPLENSKEAVLTAEVINEVTKRFSEVAKDLEINRKRRLEGRLPINAILLRGGGYMPQIEPVRERYNIKAAAIAGVALIRGVARAVGMDVYTARGLGGTKDDMFDEAVRLAVELMGRYDLVFLHVKGTDSASHDGDFKGKVSVIERLDKALTPYLDKLLNNYVVITSDHATPISIKEHTGESVPILLYGPDVVTDDVSKFSELTCWRGALGRIRGIDVMPQ",
    "response#3": "IDLAIEDGSFFQNEAFQRALDRARQPGRTLHLMGLIGDGGVHSHQRHLLALLELAARAGISRVAVHAFTDGRDTAPTSGIEHLRELLAALDRLRTGFVATVSGRYYAMDRDKRWERTKLAYDAIVCGLGQTARSPLEAIERSYAQGITDEFIVPTVIVDAEGKPLATINDGDAVIFFNFRADRARQLTQALTDAGGFTAFPRCRWPRDLLMVTMAEYR"
  },
  {
    "reference": "MYPPEFSYVRAESLQEALKFLEGNDNTRPLAGGQSLIPMLKLRVLSPDYILDINRLNELNYVKTSLNGVSIGALTRYHDILSNDIVKSKVPLMHHATRTIGDMQVRNMGTIGGAISNADPASDMPVVLTALNATIILSSASGSRSVKALDFFKGPFTTDTNKGELVTQIEVPVLDGYKTVYKKVVRRAGDYALASVALAIKLKGNEIEDIKLAYGGVHDKPFRAMEVEKNVIGKKLNDDLVKDIASKVSSQINPPSDHRGSSWYRREVVKVLTMKAFKEVA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be able to perform glyceraldehyde oxidoreductase activity, flavin adenine dinucleotide binding, FAD binding, oxidoreductase activity, acting on the aldehyde or oxo group of donors under specific conditions.2. For general function, the protein need meet that Component of the glyceraldehyde dehydrogenase which is involved the nonphosphorylated Entner-Doudoroff pathway; It is not known which acceptor is utilized in vivo.; When the artificial electron acceptor 2,6-dichlorophenol-indophenol (Cl2Ind) is used, the enzyme shows a broad substrate range (glyceraldehyde-3-phosphate, formaldehyde, acetaldehyde, propionaldehyde and isobutyraldehyde), but is most active with D-glyceraldehyde3. The designed protein should contain one or more FAD-binding PCMH-type domains that are essential for its function.4. The protein must be engineered to have a cooperative binding mechanism with FAD to ensure proper regulation. The designed protein sequence is ",
    "response#1": "PHKVYFKFGCLQFALKDLKDLKKKRAFIVTDSDPYNLNYVDSIIKILEHLDIDFKVFNKVGREADLKTIKKATEEMSSFMPDTIIALGGTPEMSSAKLMWVLYEHPEVKFEDLAIKFMDIRKRIYTFPKLGKKAMLVAITTSAGSGSEVTPFALVTDNNTGNKYMLADYEMTPNMAIVVKFFDYTFASVGVSPKELSNFDYAMVEANQGEHAGYYPGNTKLHLRVYFDKTNRRIIRAAAVGMKGVDKRIDVLSMAMMHKLTIDELVEFEVAYAPPYEK",
    "response#2": "MKVVINGFGRIGRLVLRQILKRNSSVEVLAINDLVPGDALTYLFKFDSTHGRFPEDVRCEADHLIVGKRKIQFLSERNVQNLPWKDLGVDLVIECTGLFTKESTKAHLDAGAKKVIITMKVVINGFGRIGRLVLRQILKRNSSVEVLAINDLVPGDALTYLFKFDSTHGRFPEDVRCEADHLIVGKRKIQFLSERNVQNLPWKDLGVDLVIECTGLFTKKEDAEKHIQAGAKRVLISAPGKGDIPTFVMGVNHKTFNPEKDFVISNASWVESNSNETIEVINPATEEVIGKVAKGNKADVDKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERVHYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAAFAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRMMSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTGQVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYINKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYNDLDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQSGLGREWGDYGIEEFLEVKSVLG",
    "response#3": "MKVVINGFGRIGRLVLRQILKRNSSVEVLAINDLVPGDALTYLFKFDSTHGRFPEDVRCEADHLIVGKRKIQFLSERNVQNLPWKDLGVDLVIECTGLFTKKEDAEKHIQAGAKRVLISAPGKGDIPTFVMGVNHKTFNPEKDFVISNASCTTNCLAPIAKVLLDNFGITEGLMTTVHAATATQLVVDGPSKKDWRGGRGCLQNIIPASTGAAKAVTLCLPELKGKLTGMAFRVPIEDVSVVDLTVRLDKSTTYDDICKAMKQASETDLKGILDYTDEQVVSSDFIGSEYSSIFDALAGIALNDRFFKLVAWYDNESGYSNQVVDLAAYVGKA"
  },
  {
    "reference": "MAGPRGALLAWCRRQCEGYRGVDIRDLSSSFRDGLAFCAILHRHRPDLLDFQSLSKENVFENNRLAFEVAEKELGIPALLDPNDMVSMSVPDCLSIMTYVSQYYNHFTSSGQAAASPPKPGKDPAAPSPTSTSPAVQPGEEAQGDDLSPDSLSEQGKPQPPSSACAACGQRVHLVQRYLAEGRLYHRHCFRCRQCSSTLVPGSYSSGPEEGTFVCAERCTRLGLGGRSGTRPLSLQKQQPAAAAEAKDGEDSDLSKSVVVVAAEADGLQASSEVQPHTLTKPPLPSKPQDLASPPVSRPTPAPRKASESSALTPPTPRPRSSLQQDGMVEQSVSGDLVNGRLQELPVPKPRGTPKLSERMTAPRKDPPWITLVQTEPKKKPAPLPPSSGPGPLSQASRQVENGGLEEKTQKSPAAEPEPKPYNPFEEEEEEEEASVPAVPSPAPAPPETTPKSLHPWYNITPTSSPKTKKRPAPKAPSASPLVLHASRLSHSEPPSATPSPALSVESLSSESSSHTANTEPSEPPAVPKSSSDPAVHVPGTPGTSANSVTPSAHSSLSSSGELGQPSGEQMPQARTRGSPGTHSTKPFSGATPTPFLLAGDQKSPAPPMGSSSPQMLIKSSCKENPFNRKPSPSTSPTVRKATKGAKPVRPPAPGHGFPLIKRKVQADQYIPEEDIYGEMDSIERQLDALEHSGVLLEEKLRGGANEGSEDDMLVDWFKLIHEKHLLVRRESELIYVFKQQNLEQRQADVEFELRCLLNKPEKDWTDD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that May indirectly play a role in neurite outgrowth.; Probable lipid-binding protein with higher affinity for phosphatidic acid, a lipid enriched in recycling endosome membranes2. The protein's functional design necessitates the inclusion of a NPF1 motif.3. The designed protein must have at least one LIM zinc-binding, Calponin-homology (CH) domain.4. The designed protein's functionality will benefit from Polar residues, Basic and acidic residues compositional bias in its structure.5. The protein must contain a signal peptide for proper functionality.6. The designed protein should be involved in receptor-mediated endocytosis, slow endocytic recycling, neuron projection development by localizing to late endosome membrane, endosome membrane, recycling endosome membrane, late endosome, extrinsic component of membrane and exhibiting identical protein binding, small GTPase binding. The designed protein sequence is ",
    "response#1": "AGPRGALLAWCRRQCEGYRGVDVRDLSSSFRDGLAFCAILHRHRPDLLDFDSLSKDNVFENNRLAFEVAEKELGIPALLDPNDMVSMSVPDCLSIMTYVSQYYNHFV",
    "response#2": "NAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIEREHQISPGDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLL",
    "response#3": "TLPVAAAFTETVNAYFKGADPSKCIVKITGEMVLSFPAGITRHFANNPSPAALTFRVINFSRLEHVLPNPQLLCCDNTQNDANTKEFWVNMPNLMTHLKKVSEQKPQATYYNVDMLKYQVSAQGIQSTPLNLAVNWRCEPSSTDLRIDYKYNTDAMTTAVALNNVQFLVPIDGGVTKLQAVLPPAVWNAEQQRILWKIPDISQKSENGGE"
  },
  {
    "reference": "MPTLLIASGNAHKAEEIAAILGGGFSVQTLASFPSAPKVVEDGGTFSANAIKKAVEIAVWLGGQAIAQALPNFVLADDSGLEVDALGGAPGIHSARYAGGDSNASDKANNAKLLHELADAADGKRGAQFQCVLALVKLGQEAEVQTFKGICRGSIAEVQTGQGGFGYDPLFIPDGHTQSFAELGREVKNEISHRARALAKLAEHFSQGLSASQSPHNGDHQPNQ",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The substrate, Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..3. We need a protein that can perform dITP + H2O = dIMP + diphosphate + H(+) with high efficiency.4. A protein with metal ion binding, ribonucleoside triphosphate phosphatase activity, nucleotide binding, dITP diphosphatase activity functions is required to enable nucleotide metabolic process, purine nucleoside triphosphate catabolic process. The designed protein sequence is ",
    "response#1": "MIKVVLASNNKGKIAEFNRLMKDVDFEVVPQSEFNVPEAEETGLTFVENAILKARHAAAISGLPALADDSGLMVDALNGAPGIYSARYAGENASDADNNAKLLRELAGVPDAQRGAHFYCVLVLMRHAADPLPVIAEGVWHGRILDTPRGEGGFGYDPLFYVPEQQCTSAQLPADEKNRVSHRGLALERLLATLKS",
    "response#2": "MQKIVLASGNKGKLKEFNALLAPLGIEVLPQSAFDVPECEEPHVTFVENALAKARHASRITGLPALADDSGICVAALGGAPGVFSARYAGEPKSDARNNEKLLHELAGVPDEQRGAHYYCVLVLLRHSDDPQPLIAEGRWHGQILHAPRGDGGFGYDPVFFVPQLNCSSAQLTSEEKNSISHRGQALRQLLDKLRSV",
    "response#3": "MKTFFATGTDTGVGKTVATAALLRALRERGHPAVGMKPVAAGCERRGGRLVAGDALLLQRAAGAESLDDVCPIALPEPLAPAVAAELEARPRPIEPARLVAAWRALAARHDAVVVEGAGGWLVPLTDRELTVADLARDLGLPVVLVVPVRLGAINHTALTIEALAARGLTVLGVVANRVARGAPGEDVQTTAEALAERTAVPLLGTVPWLPDLDEDGLVARFDRWLDLPALLG"
  },
  {
    "reference": "MPHKVSLSRLKLTDFRNYAAASLSLDGRHAVLTGDNGAGKTNLMEAVSLLSPGRGLRRAAYGDITRVGAAGGFSIFAALDGMEGEVEIGTGIEAGEETTARRLRINGTPAKTADELTDHLRLLWLIPAMDGLFTGASSDRRRFLDRLVLSLDPAHGRRASDFERAMRSRNKLLDDGRFDPSWLAGIEEQMASLGIAMALARQEMLGLLTRLTEETLESSPFPSASLQLSGFMDGQFSRPSVDLEDDYRVMLAESRYRDAGAGRTLEGPHRTDLVVHHREKAMEAARCSTGEQKALLVGLVLAHARLVGNLTGHAPILLLDEIAAHLDENRRAALFDIIDGLGGQAFMTGTDRGMFTALGDRAQFFTVADGRVFG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be able to modulate DNA repair, SOS response in a way that leads to a desirable outcome.2. For general function, the protein need meet that RecF binds preferentially to single-stranded, linear DNA The designed protein sequence is ",
    "response#1": "GIPYVQAPSEGEAQAAFMVQRGDARFAVSQDYDTLLFGAPLLMRNLTVSGKRKIRGRAVTVNPERLVLSEVLSGLSLTREQLVEVGILVGTY",
    "response#2": "MGVNLKEIVDPVKKEIEFKQLFGKVIAIDAFNALYQFLFSIRQDGEPLRDSKGRITSHLSGLFYRTINLLEYGIKPIYVFDGTPPKFKIVAWEKRKKHKEV",
    "response#3": "REQLIAIAMLLGTDYNEKVPGIGPKTAIEIVKRF"
  },
  {
    "reference": "MRAYYTADEVRAAEAELFTRVPAGMPMRRAAFGLADVMARELRERTGGVAGRAVTLLVGSGDNGGDALWAGSFLRRRGVAVRAVLLAPKRAHAEGLAALRRAGGRVVSAPSAPEDLAADRWPPEAGAHGEKGRDVPWPVLELLGKPDLVVDGIVGISGHGSLRPVAAKIVAGVRCPIVAADLPSGVDPDTGAVSGPAVTADVTVAFGAYKPVHVLAAPHCGRIELVPIGLQLPSANLVALEPRAVGARWPVPGAADDKYTQGVTGVCAGSATYPGAAVLCTGAAVAATAGMVRYAGTGAAQVLAHFPEVIAAESVSDTGRVQSWVFGPGAGTDADARDRLAEILATDLPVVVDADGLTLLAAEPGLVQGRQAPTVLTPHAGEFARLTGSEPGLDRVAAVRKLAESWQVTVLLKGRATLIASPDRPVFVNEAGGSWAATAGSGDVLSGIVGALLAGGAHPVIAAASAARAHALAANLAAHASGTAGAPISASPLLSHLREAIATLRELAAA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The AMP, (6S)-NADPHX binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.3. The protein contains novel YjeF N-terminal domains that confer a unique function or activity.4. The designed protein must possess ADP-dependent NAD(P)H-hydrate dehydratase activity, NADHX epimerase activity, metal ion binding, ATP binding, NADPHX epimerase activity to support nicotinamide nucleotide metabolic process under certain conditions.5. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.6. For general function, the protein need meet that Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.; Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP The designed protein sequence is ",
    "response#1": "MMITDINCECLGLSRLCLMESAGKSLAEEVGKIAVYTFSKPVKIAIFTGSGGNGGDGFVAARYLLNRGYDVDIYMLTDKDNIHSDNAKTNFEIITNMEPRLSHLTVHYLDTIEDVNNCDIAQSESFSEFIIVDAILGTGIKGELRPKVKRAVEVINKSNGLTVSVDVPSGMDPLTGEVSDVAVEPHYTISFHRVKTGIHLAGEDKVGGIVTCDIGIPIEAEGIIGGNTGMNGALQLAGASALYSGCGKVSMIPLDQSFKADMSMPELMTKSLKNVPKNINIFSALAVGVGFDTTDDSQQILESIIDNLTQLAIFDADALNIIVTNQQIREKFINLENKIITPHPAEAARLLDCTTKEIQEDRFTAIRTLAKKYNATVVLKGAGSLICKDNKIYINTTGNQGMAVAGQGDVLSGIIGAFLAQGLNTLSASRLAVYVHGLAGDNVAKKLDSEVVRMFLPIRPEDGNKSTFGTVTIVGGSEKYIGAPKLSGIAAFKAGCGYVNMVAPEIIRNLNAGIFSQAIWQPADFNSDEGIEIALHSIRDMSKKKGSLVIGPGLGQSPKVFDLVHRILISWKENPVPLLMDADGLNALPHIPDWQKNIPDCFVITPHEGEMARLCNMTVDEIKTNRIQIAEQKSKEWKTVIVLKGAFTVIASPDGYTAVMPYANSILAKAGSGDILSGLIGGFLAQGSLPFEASCLGVWVHAKAAQIAFRQIGQPYSMSADDLLSLIPEAIRMIYE",
    "response#2": "MMITDINCECLGLSRLCLMESAGKSLAEEVGKIAVYTFSKPVKIAIFTGSGGNGGDGFVAARYLLNRGYDVDIYMLTDKDNIHSDNAKTNFEIITNMEPRLSHLTVHYLDTIEDVNNCDIAQSESFSEFIIVDAILGTGIKGELRPKVKRAVEVINKSNGLTVSVDVPSGMDPLTGEVSDVAVEPHYTISFHRVKTGIHLAGEDKVGGIVTCDIGIPIEAEGPGDFKVLWKPRPDESRKGDFGRLLVIGGSENFTGAPAFAALAATKIGTDLVYVAAPQKTAEIITSYSPDLISIKLPGEHLSPRALPELSRWTSNMDAIVLGPGIGIHDETADSVWKLISEAESLKKPLVLDADALKVFGRRRRKLRTPCIITPHAGEFAAVAQRKLSSEIELNKEAAAQLAKELGSTVVLKGHVDIIANADGRVRLNRTGNPYMTVGGTGDILSGLAGGLLAQHVEPFRAGCAAVFLNGLAGDIMISEKVPTITPSALVDYLPKAMRYGVLAGSATYPGAAILSTGAAVAATSGMVRYAGSAAHDVVNHWPEVVATADTESVGRVQAWVVGPGLGTDESAAAALRLALSSDLPVIADADALTLLAAAPDLVVNRSAPTVLTPHAGEYQRLAGADVGSDRVGAARTLADTLGATVLLKGNVTVIASRGGIYLNPAGQSWAATAGSGDILSGVIGALLAAGIPATEAAAMAAFVHARAAALAAAHPGPNAAPTSAPSLWLHHAATPLTAHHKYDRGHAVVFSGGMSQTGAARLAALSALRGGAGLVTVFSPASALLVNAQHLTGIMLKRCEDGDLDVLLEDKRLNAFVLGPGFGIGDRAKAMALTVLKAGRRLVLDADGLTSFRDHASQLFEAARQCSHSEPQLVLTPHDGEFARLFPDLAGDLRLSKVDRARHASRRSNAIVVLKGADTVIAAPDGRAAINATGTPWLATAGTGDVLAGLVAAQLAQKRPSFEAACRAVWMHGKAAEHFGPGLISEDLPHCLPAVHRELV",
    "response#3": "MMITDINCECLGLSRLCLMESAGKSLAEEVGKIAVYTFSKPVKIAIFTGSGGNGGDGFVAARYLLNRGYDVDIYMLTDKDNIHSDNAKTNFEIITNMEPRLSHLTVHYLDTIEDVNNCDIAQSESFSEFIIVDAILGTGIKGELRPKVKRAVEVINKSNGLTVSVDVPSGMDPLTGEVSDVAVEPHYTISFHRVKTGIHLAGEDKVGGIVTCDIGIPIEAEKITELLEVLPAREQHTHKGKQGRALLVGGNKGFGGAILIASQACARVGAGLTSVITHEDYLNSVILHQPEVMAHTYNPIESNTLFEMADVIAIGPGLGKDEWAERLLKRTLNENCFKVLDADALNILAENPILIESLDTNTLITPHPGEAARLLDCSTTDIEEDRIKAIKALRAKLNCHVTLKGSGTLITHPNTDLITVCRYGNPGMAAGGMGDALTGIAAALIAQTYQNQSSINDVIELAVCLHSHAADEEAAAHGERGLLASDLADRARHILNRGPGDVWAALPNRKSDSHKGDFGRLLIIAGSRQYCGAPVLMTHAAIKSGVDLVTSAVPQYVADHQVFPADAIVHPIKGEIFTSESVDELLALNYDAAAIGPGLGMADETRDALNLFLRKSNKPRLLDADAIKLADKKRLNPQTIVTPHAGEFKMLTGEYDPKKREQLTEKYAKKLGCTILLKGPTDVISDGKTTRLNRTGNPAMTKGGTGDTLAGLAGGLLAQNKNPFQTACAAAFLNGLAGDIAYKDLNVMLTASDVAAYLPKALAECAATLRSLLPRRQFEAHKNQFGRIGIVAGSRGFAGAATMCAWGALRAGAGLVEVFVPENIYEVVAASAPFESMVKPVTSYRDLLDEKIDVWGIGPGLGKENAREIIDLVQRTKNAMVVDADALNLISREVSILKNCRGPRLLTPHPGEMKRLAGDRKISRAKLAKNFTAEFPVTLLLKGSRTVVAENGKRISYNTTGNPGMATGGMGDVLTGVCASLIAQNLSPFDAARVGAWVCGRAAELAIFNCGESEQSLLPRDVLTHLGRAFRE"
  },
  {
    "reference": "MMIKQLPENDKPKEKLIAKGAAHLADSELLAILINTGRKGHSSIEVAQDLIKMARSLKELKLLSLNDIMKVKGIGLNKAIILKAAFELGERMYIPDLDTKVKITSPQDAADYFLSRMMHLTHEQFEVLFLNSKNVVIRHEVIFVGTLNSSIVHPREVFKAAIKWSSNAIIVVHNHPSGDVTPSKEDILTTKRLQECGRVLGIELLDHIIIGDAKYLSMVEGGYFDD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should contain one or more MPN domains that are essential for its function.2. The protein must exhibit metal ion binding.3. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects.4. The designed protein should contain at least one JAMM motif motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "LTSPELTREYLSAHLRHYPYEVFAALFLDNQHRVIAYEELFRGTIDSASVHPREVVRRVLKHNAAAIIFAHNHPSGVAEPSLSDKEITETLRKALRLVDVRVLDHMIVGDGEVVSLAERGR",
    "response#2": "IDSPGAVEDYLRLKIGARPYEVFAAVYLDARHRLIDMEEITRGSLTRMAVYPREIVRRAMVHNAAALIVAHNHPSGAVQPSAEDRRLTRVLHDALALVDVRLLDHVVIGISDTFSFARAF",
    "response#3": "MKIQLGCTQDAQQLLYAQLRHHESEVFACLFLDNRHRIIQFEKLFYGSINQASVHPREIIKRALYHNSAALIVAHNHPSGVPDPSQADRAATTHLKEALALIDVRLLDHIIIGDRNSFSFAESGLI"
  },
  {
    "reference": "MSAEEMVQIRLEDRCYPVSKSKLIEQSDYFRALYRSGMREAVRPEVGPEVQQLRGLSAPGLRLVLDFINAGGAREGWGLSEDELAEASVLSEMVEAASFLQVTALLRLLLSHVRLGNCLELYRLAQVYGLPDLQDACLRFMVLRFHQVLCQPQFPLLLSPPQAPGDCSLKQRLREARMRGTPVLVALGDFLGGPLAPHPYQGEPPSMLRYEETTERWFPLANNLPPDLVNVRGYGSAILDNYLFIVGGYRITSQEISAAHSYNPITNEWLQVASMNQKRSNFKLVAVNSKLYAIGGQAVSNVECYNPEQDAWNFVAPLPNPLAEFSACECKGKIYVIGGYTTRDRNMNILQYCPSADLWTLFETCDVHIRKQQMVSVEETIYIVGGCLHELGPNRRSSQSEDMLTVQSYNTVTRQWLYLKENTSKSGLNLTCALHNDGIYIMSRDVTLSTSLEHRVFLKYNIFADSWEAFRRFPAFGHNLLISSLYLPNKAET",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should take into account the physiological conditions of the Protein modification, protein ubiquitination pathway.2. For general function, the protein need meet that Involved in microtubule dynamics throughout mitosis .; Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis; The BCR(KLHL42) E3 ubiquitin ligase complex mediates the ubiquitination and subsequent degradation of KATNA13. The designed protein should have a specific function within the spindle, cytoplasm, Cul3-RING ubiquitin ligase complex. The designed protein sequence is ",
    "response#1": "CLDMQDFAEAFSCSGLASAAQRFILRHVGELGAEQLERLPLARLLRYLRDDGLCVPKEEAAYQLALRWVRADPPRRAPHWPQLLEAVRLPFVRRFYLLAHVA",
    "response#2": "CLDMQDFAEAFSCSGLASAAQRFILRHVGELGAEQLERLPLARLLRYLRDDGLCVPKEEAAYQLALRWVRADPPRRAPHWPQLLEAVRLPFVRRFYLLAHVI",
    "response#3": "NFPTATQEYVCPSHGSHVICTCCFQPMPDRRAEREHNSHVAPQQCTICLEPFCHMYWGCNRMGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLASRGLTWKDMLNESLAAVQRGVFMLPDYRINGTTVLCYFCGLRNFRILTYQYRQNC"
  },
  {
    "reference": "MEAPRILLVVGGGIAAYKACELVRLIKKGGGEVTCVLTEGGAQFVTPMSLAALSGRKVHTSLWDLKDEAEIGHIQLSRSADLVVVCPATADLLAKMAAGIADDLATTLILATDKPVLAVPAMNVRMWEHAATQRNIAWLLQAGVRVMQPDDGEMACGEFGAGRLPEPETVWLEIADALGLDPGEAGAGEIAGYLEALEPEDELELEPEQGGRMGGLAGLLASIIPRSTARRIREEEVSYQEGAYEEAAYLADEADLPVELAELTPLPEPDLAAAGPLLASKGKAKSAPPTDRGAINHEVDPNRDAPDPVGDGAAPAFDPPPFDPLAGQPDFEDDPAHRPLYGKHVLVTAGPTYEAIDPVRYIANRSSGKQGYAIAAAAAAAGARVTLVSGPVVLATPPGVERIDVESADEMAAAVKAALPADAAVMVAAVADWRTRDYAPEKIKKRGSAPPALLLAENPDILAQLAASPHRPDLVIGFAAETEDVVKNAIRKRKSKGIDWIVANDVSGPQGESAMGGDANTVHLISEDATEDWEPMSKQAVARALVERIAEKLANG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for CTP binding.2. The Proton donor of the protein must be engineered to enable effective catalysis of H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.3. The protein must exhibit the following characteristics: Catalyzes two sequential steps in the biosynthesis of coenzyme A; In the second step the latter compound is decarboxylated to form 4'- phosphopantotheine..4. The presence of DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.5. The designed protein should be optimized for metal ion binding, phosphopantothenoylcysteine decarboxylase activity, FMN binding activity in order to improve coenzyme A biosynthetic process, pantothenate catabolic process.6. The protein requires Mg(2+) to activate a critical catalytic site.7. We require a protein that can specifically bind to the metabolites produced in the coenzyme A biosynthesis, Cofactor biosynthesis pathway. The designed protein sequence is ",
    "response#1": "KRILLGITGGIAAYKSAELVRLLKKAGCEVRVVMTRAAEAFVTPLTFQALSGEPVRTSLLDPEAEAGMGHIELAKWADQVVVAPASADFLARLAQGLADDLLTTVCCATEAPIILAPAMNQAMWRNPRTQRNVALLRDDPQVCLWGPDQGEQACGDTGPGRMLEPAELAARLLPLLESQPANLAGRHVLLTAGPTHERIDPVRYISNHSSGKMGFAIARAAAAAGARVTLVAGPVQLATPPGVERIDVESAAQMHRAVHAAVDAADIFIATAAVADYRPAATAAHKLKKDGQAMSLNLEQTTDILASVAVLPDAPYTVGFAAETHDVERYAREKLAKKKVDLVVANRVGEEGGRTIGFGADDNEVTVITSGGTEAWPRMAKGQVARRLAQRIADTLG",
    "response#2": "MGYRALITSGPTREPIDAVRFIGNRSSGRQGAAIALELARRGMLVDYISGPSSIPTDHPGIRRVDVETALDMKAAAEAAMAHALPDAVIGTAAVADVRPATTISGKAAKTDLPDAISMVPNPDILAGINAVVSPECFKVGFALAHGNGQEAALRKLEAKNCDMIVLNSITDKGAGFEHTTNRVEWSGGIMLSGKTVFVTSGSTAESIDPIRLLTNRASGKTGDALAKEAYIRGAEVYLFHRGKSSWGHLPHFHDIHTESVQEMIEAVTEKIALADIPLSGKNILISAAAISDYTVEAADSKIKSGERELTIKLKPTKKLIEEACYTDPNIFIVGFKAETDVSYQELINSAASKIDAGIADVVVANDVKEKGMGTNENDVYLLSEEGELELSGKTVFVTSGSTAESIDPIRLLTNRASGKTGDALAKEAYIRGAEVYLFHRGKSSWGHLPHFHDIHTESVQEMIEAVTEKIALADIPLSGKNILISAAAISDYTVEAADSKIKSGERELTIKLKPTKKLIEEACYTDPNIFIVGFKAETDVSYQELINSAASKIDAGIADVVVANDVKEKGMGTNENDVYLLSEEGELEKVPISSKXEAASAILDKAIKL",
    "response#3": "KRILLGITGGIAAYKSAELVRLLKKAGCEVRVVMTRAAEAFVTPLTFQALSGEPVRTSLLDPEAEAGMGHIELAKWADQVVVAPASADFLARLAQGLADDLLTTVCCATEAPIILAPAMNQAMWRNPRTQRNVALLRDDPQVCLWGPDQGEQACGDTGPGRMLEPAELAARLLARDALAFFQPRLSGKTVFVTSGSTAESIDPIRLLTNRASGKTGDALAKEAYIRGAEVYLFHRGKSSWGHLPHFHDIHTESVQEMIEAVTEKIALADIPLSGKNILISAAAISDYTVEAADSKIKSGERELTIKLKPTKKLIEEACYTDPNIFIVGFKAETDVSYQELINSAASKIDAGIADVVVANDVKEKGMGTNENDVYLFWPGDESLAGRHVLVTSGPTHEPIDPVRYIANRSSGAQGTAIAAALRDLGARVTFVTGPASVPPPPGVDVVAVGTAAQMAAAVRAALPADAAVMAAAVADWRIANPGTQKMKKDGSDRPPALDFAENEDILAAVSRGEPRPRLVVGFAAETEKVVEHATAKRLRKGCDWILANDVSPETGIMGGAENAVTLSLNWQERPLAGRHAVVTSGPTHEPIDPVRYIANRSSGRQGSAIAAALVRRGARVTFVTGPAEAPRPNGAEVIEVETAAEMRDAVLGTLPAEIAIFAAAVADWHVRTPSASKVKKAADGAPPVFELGENPDILRTVAGLAAGRPGLVIGFAAETDDVLANATAKRLRKGCDWLVANDVAPASGIMGGTENAVTLIAH"
  },
  {
    "reference": "MLHTDSPSLDTRVPLIGLDRRALEKELVKLGEPEYRAKQIWHWLYHRGAQTFDDMTTLPRGLRELLHTNCTIQRPKIKRLQTSEDKSRKWLVEFADGNEVECVHIPEEDRGALCISSQVGCTLTCKFCHTGTQKLVRNLTSREIVEQFLVARDTIDEWPSSSDQRMISNVVMMGMGEPLYNYENVSRALXIVMDGEGIALSKRKITLSTAGVVPLFKRCGXELGVNLAVSLHAVTDDVRNILVPINKKYPIAELLNACRSYPSGKNSRRITFEYVMLQGINDSDSDARALVRLLGGIPAKVNLIPFNEWPDAPYKCSSNNRMHAFADIVNSGGLSAPIRVPRGRDILAACGQLKSASKRIKKTVRP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The ideal protein would be one that plays a critical role in rRNA base methylation through rRNA (adenine-C2-)-methyltransferase activity, tRNA (adenine-C2-)-methyltransferase activity, metal ion binding, rRNA binding, tRNA binding within cytoplasm.2. The protein contains novel Radical SAM core domains that confer a unique function or activity.3. The designed protein should have a high specificity for S-adenosyl-L-methionine, [4Fe-4S] cluster binding, with minimal off-target effects.4. For general function, the protein need meet that m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.5. The protein's S-methylcysteine intermediate, Proton acceptor must be carefully engineered to ensure that the amino acids involved in adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "MNGKVNLMGMSASSLADLMEMMGEKRFRATQILKWIHQMGVRDFDQMTNLSKVLRGKLSEGMEIVVPEVVSCENSSDGTRKWLVQVENDSCIEMVFIPEGSRGTLCVSSQIGCALDCSFCATGKQGFNRDLTASEIIGQLWIAADSFNQFSSTTTRRITNVVMMGMGEPLMNFDNVVEAMSIMLDDHAYSLSKRRVTLSTAGVLPALDRLGDVLDVSLAISLHAPNDELRNKLVPLNKKYSINEFIDSARRYLSKMPDSRRKVTVEYTLMDQVNDRASHALELSSVLRELPCKINLIPFNPFPGTEFRRVSDVAMNRFRDILHREGYTVTVRTTRGDDIAAACGQLAGLVNDKTRRQEWSRLKRE",
    "response#2": "VGRSGALCVSSQVGCTLNCTFCHTGTQALVRNLTAAEIVQQVLIAKDDLAEWPSRLEDRQITNVVFMGMGEPLYNLDHVADAIDVISDGEGIGIGRRRITVSTSGVVPKIEELGARTNTMLAISLHATNDALRDELVPLNKKYPIAELLDAIRAYPELSNSKRVTFEYVMLKGVNDSPGEAKALVRLLQGIPSKINLIPFNPWPGSPYECSDWATIERFADIVNKAGYASPIRTPRGQDIMAACGQLKSATERARRAK",
    "response#3": "SQIGCALDCSFCATGKQGFNRDLTASEIIGQLWIAADSFNQFSSTTTRRITNVVMMGMGEPLMNFDNVVEAMSIMLDDHAYSLSKRRVTLSTAGVLPALDRLGDVLDVSLAISLHAPNDELRNKLVPLNKKYSINEFIDSARRYLSKMPDSRRKVTVEYTLMDQVNDEMQAASDNPHGSSQVGCTLTCSFCHTGTQKLVRNLTAGEIVGQIMIARDDLGEWPLPGRNPKNETRLLSNIVLMGMGEPLYNFEAVRDAMKIAMDPEGISLSRRRITLSTSGVVPEIARTAEEIGCMLAVSFHATTDEVRDKLVPINKRWNIATLLDALRDYPKASNSERITFEYVMLKGVNDSDEDARRKRIMTRDQLLAALEAAEA"
  },
  {
    "reference": "MKLKELLKEMDILRLDGDLETEISGLENDSRLIRPGYLFVAVKGYETDGHLFCRQAILNGAAAVLLEQEEGLEEICREHGVAVVKAADSRKALAQAAAAFYGWPARQLNMVGITGTNGKTSTAILSYRILKAAGHLTGVIGTISNFIHNRELPAERTTPEAHQLQKLLAEMRAESVDTAVMEVSSHALELDRVCGIRFKVAVFTNLSLDHLDFHQTMENYLKAKSRLFQMAETGVINADDPVKDELLKGNTCQKLYFYSIKDPQADFYADQIEHHLTGTNYRLSFAGRTYPVVLRTPGNFSVYNSLAAIAITYSLGVPMEVILPALAECSQVRGRFQPVQLPGGFTAIVDYAHTPDGLENVLRSINEFKTGKVITVFGCGGDRDRSKRPLMAEIAERYSDYVIITSDNPRTEEPEQIIAEVAAGMTDRAYELETDRRRAIEKALTRAEAGDVVLVAGKGHEDYQIIGRQKQPFDDVKIIEEWGSKQC",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must possess magnesium ion binding to support cell division under certain conditions.2. The designed protein should have a binding site that is specific to the target meso-2,6-diaminoheptanedioate ligand.3. The designed protein should interact with enzymes and substrates within the peptidoglycan biosynthesis pathway to enhance or regulate its activity.4. The designed protein should contain one or more Mur ligase C-terminal domains that are essential for its function.5. Mg(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "VTGTNGKTTTATLLYEMARLMGYKAGLLSTVKNVVDRTEYPAKQTTPGSLEINRFLHEMVQAGCDYAFMEVSSHACVQYRIDGLHFAGGIFTNLTRDHLDFHKTVENYIKAKKRFFDLLPPDAFALVNADDKQGSVMVQNTKARSYTYSLRAMADFKGRVIEDRLDGMTVNFNGQELEVRFTGRFNVYNLLSVYG",
    "response#2": "ITGTNGKTSCVEMTRQLWRMAGFHAASIGTLGVTTADERVTTGLTTPDVVTFLSNVAGLAREGVTHLAFEASSHGLTQYRTEGLKVAAAAFTNLSRDHLDYHGDMAAYFTAKIRLFSEVLSTDGAAVVWADDPQSARVIDLARERGNRLITVGTQGETLRLVGRDPTLLGQGLTIEADGQTYKVTLPLIGAYQAANALVSAGFRNARWIGGNCTVSCMLMGITGTNGKTTIASLLYELFMSLGKGVGLLSTIKIMINDREFKATHTTPDSLTINRSLAEMVKDGIEYCFMEVSSHGIHQNRIMGLDFVGGVFTNLSHDHLDYHQTFAEYRDVKKRFFDQLSKDAFALTNIDDKNGSIMVQNTKAKRLSYALKTLGDYKAKILEKSFSGSLLTINGVEIWTKLIGNFNAYNVLAIYGWGAAEPLRRMLRI",
    "response#3": "VTGTNGKTTCVQLLMQLFNQLGEKAASIGTMGYGFSQSALQDFGLTTPDAIACQRVLAQLRNAGATFVAMEVSSHALEQGRVDAVNFEGAVFTNISRDHLDYHGTFEHYIAAKSRLFTFDALRFAVINGDDSASRALSAASTAESSFLYGAESGNTVVAKDIALSPEGVAAKISSAWGEANIQSCLIGNFNILNLLAVITE"
  },
  {
    "reference": "MLHGKRILLIISGGIAAYKSLDLIRRLRERGTAVRCVLTDSATQFVTPLSVSALSEDRVYGDLFSLTDEAEIGHIQLSRDADLLVVAPATTNIMAKMAQGIGDDLATTVLLATDKDVLVAPSMNVRMWEHAATQTNIATLKSRGIWFAGPTEGAMACGEFGEGRMVEPLEVLAEIESYFTTSAPLAGRRAIVTSGPTLEAIDPVRYIANRSSGKQGHAIAAALAHMGAETILVTGPTNETAPAGVDVRHIESAREMYDVCVGALPADIAVCAAAVADWRPARQGDRKIKKEKGAAPPSIEMVENPDILRTLSSAGNQRPALVVGFAAETGDLIANATAKLMRKECDWIVANDVSADTGTFGGDTNTVHLITANGSEDWPVMRKNEVADRLAERIAGRFNQSTEAVG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The Proton donor active site of the designed protein must be optimized for substrate binding and specificity.2. The protein design should take into account the physiological conditions of the Cofactor biosynthesis pathway.3. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..4. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.5. The designed protein should be able to compete with endogenous proteins or inhibitors for CTP binding.6. The designed protein should be optimized for phosphopantothenate--cysteine ligase activity activity in order to improve coenzyme A biosynthetic process, pantothenate catabolic process.7. The designed protein have Flavoprotein domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MSGGVAAFKSAELCRRLIKAGATVQVVMTESATQFMTPVTMQALSNRPVYTSQWDAREHNNMPHINLSREADAIVVAPCSADFIARLVQGRADELLSLLCLARPIDAVPLILAPAMNREMWAHPATQRNLRQVQADGAVVLGVGNGDQACGETGDGRMLEAAEILFELSRLLAPKQLKGKKVVISAGPTRERIDPVRYLTNFSTGKMGYAMAAAAHKLGAETVLVSGSVAIEPPAGVRVVHVESAEDMLQAMLAEYDDANIVVKTAAVADYRPKTAHLQKMKKQPGDAIIELERTTDILLTLGERKKDQLLIGFAAETNDVLKYAKGKLEKKNANYIVANDVTQDGAGFGTDTNTVTLITQEKEIALPVMPKEQVARALIQRIITLKAS",
    "response#2": "MSGGVAAFKSAELCRRLIKAGATVQVVMTESATQFMTPVTMQALSNRPVYTSQWDAREHNNMPHINLSREADAIVVAPCSADFIARLVQGRADELLSLLCLARPIDAVPLILAPAMNREMWAHPATQRNLRQVQADGAVVLGVGNGDQACGETGDGRMLEAAEILAAIETVLGPHDWQGRRLLVSAGPTREWFDPARCISNPSTGRMGVLLAQAARLRGAEVDLVHGALQLDPSWLEGLNLHPIDTGAQLQRALEQLQPQADAIAMAAAVADHRPARTLSYKPTKAELDAAMCGPWEPVPDLLVELVSRRPTGQRILGFAAHSGDVLPQAQAKFARKGCDLLFANPIDQPDAGFGSSTNQGWLVAQAGQITWQGRRLLVSAGPTREWFDPARCISNPSTGRMGVLLAQAARLRGAEVDLVHGALQLDPSWLEGLNLHPIDTGAQLQRALEQLQPQADAIAMAAAVADHRPARTLSYKPTKAELDAAMCGPWEPVPDLLVELVSRRPTGQRILGFAAHSGDVLPQAQAKFARKGCDLLFANPIDQPDAGFGSSTNQGWLVAENGTVTPVDRWQGRRLLVSAGPTREWFDPARCISNPSTGRMGVLLAQAARLRGAEVDLVHGALQLDPSWLEGLNLHPIDTGAQLQRALEQLQPQADAIAMAAAVADHRPARTLSYKPTKAELDAAMCGPWEPVPDLLVELVSRRPTGQRILGFAAHSGDVLPQAQAKFARKGCDLLFANPIDQPDAGFGSSTNQGW",
    "response#3": "MSGGVAAFKSAELCRRLIKAGATVQVVMTESATQFMTPVTMQALSNRPVYTSQWDAREHNNMPHINLSREADAIVVAPCSADFIARLVQGRADELLSLLCLARPIDAVPLILAPAMNREMWAHPATQRNLRQVQADGAVVLGVGNGDQACGETGDGRMLEAAEILAVAKAYFEGRLDLKGKKVVISAGPTRERIDPVRYLTNFSTGKMGYAMAAAAHKLGAETVLVSGSVAIEPPAGVRVVHVESAEDMLQAMLAEYDDANIVVKTAAVADYRPKTAHLQKMKKQPGDAIIELERTTDILLTLGERKKDQLLIGFAAETNDVLKYAKGKLEKKNANYIVANDVTQDGAGFGTDTNTVTLVTRDGVEEWPRLSKEAVAERLAERIAEFFA"
  },
  {
    "reference": "MPLNKDIKKVLVIGSGPIIIGQAAEFDYSGTQACQALKEEGIEVVLVNSNPATIMTDKEIADKVYLEPLTVEFVEKVIEKERPDSLLAGMGGQTGLNLAVELYEKGILDKYNVKVIGTSIESIKEGEDRELFRDMMNRINQPVIQSEIITDLDAGIAFARKIGYPVIVRPAYTLGGTGGGIANNEEELIETLTSGLQLSTIGQVLLEKSVKGWKEIEYEVMRDSFGNCITVCNMENIDPVGIHTGDSIVVAPSQTLSDKEYQMLRSASIDIINAVGIEGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVAAKIALGYGLDEIKNAVTGMTYACFEPSLDYVVVKIPKWPFDKFQGADRALGTKMMATGEIMAIGSNFEAAFLKGIRSLEIGKYSLEHKKFKDLSMYELRERVVSPDDERIFALAEMLRRGYRIDMVSKITGIDIFFLEKFRWLVEEEQKLKQSTIDDLNREWLLKLKRRGFSDKAIADMLKVSPDEIYRLRDIWHIKPAYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDNKKVVVIGSGPIRIGQGIEFDYASVHCVMALRKQEIETIVINNNPETVSTDFSISDKLYFEPLTEEDVLNIIDKEKPDGVILQFGGQTAIKLAKFLKEKNIPTLGTTSDQIDLAEDREQFDDLLERLNIARPKGKGVWSLEEGLEEARRLGFPILVRPSFVLGGQGMEITHDEEELTYYLTNAFEKDSKNPILIDKYLMGREIEVDAISDGEDVL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Ideal protein should have a binding affinity for key enzymes in the Pyrimidine metabolism pathway to enhance the overall efficiency of the pathway.2. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.3. The protein contains novel ATP-grasp domains that confer a unique function or activity.4. The designed protein should have a binding site that is specific to the target ATP ligand.5. The designed protein should have ATP binding to facilitate arginine biosynthetic process. The designed protein sequence is ",
    "response#1": "VRISLVGKYAGLADSYMSVLEALRHAGGVCNAGVDVELVEAEELEESGASERLRASDGIIVPGGFGTRGAEGKVKAIEYARKRDVPFLGVCFGFQLSVVEFARNVALMGSANSTEVDPRTPYPVIDLMAEQKGIGSLGATMRLGASPVVVEKGTLAYKLYGREEIYERHRHRYEVNPSLKGRLVERGLRFSGYDRDGKRMEILELPGKFFFFATQYHPEFKSRPGRPDPAYYGFR",
    "response#2": "ADSYMSVLEALRHAGGVCNAGVDVELVEAEELEESGASERLRASDGIIVPGGFGTRGAEGKVKAIEYARKRDVPFLGVCFGFQLSVVEFARNVALMGSANSTEVDPRTPYPVIDLMAEQKGIGSLGATMRLGASPVVVEKGTLAYKLYGREEIYERHRHRYEVNPSLKGRLVERGLRFSGYDRDGKRMEILELPGKFFFFATQYHPEFKSRPGRPDPAYYGFVYPVIVRPAFTLGGADS",
    "response#3": "IAALDCGMKFNQIRCFVRRGAKVTVLPWSSDLTKTETNYDALFISNGPGDPIQCKGIIDQIRGWMTTKKPLFGICLGHQLVALACGFKTYKMKYGNRGHNQPCVHLPTGRCFITSQNHGYAVDTKNITDGWYELFRNKNDRSNEGLAHYTHPWMTVQFHPEHMAGPSDLEFLFDIFLEVL"
  },
  {
    "reference": "MSKPVVLSGVQPSGELTIGNYLGALRQWVKMQDDYECLFCIVDLHAITVRQDPESLRKATLDVLALYLACGIDPEKSTIFIQSHVPEHTQLAWVLNCYTYFGEMGRMTQFKDKSARHAENINVGLFTYPVLMAADILLYQANQVPVGEDQKQHLEITRDIANRFNALYGDLFAVPEPFIPKAGARVMSLLEPEKKMSKSDENRNNVIGLLEDPKAVAKKIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSGVTGKSIAELETEFEGKMYGHLKGTVADEVSAMLTTLQERFHHFRNNEALLNDIAREGAQKARERAKATLDAVYQAVGFVSL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have a low Km value for ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-   tryptophanyl-tRNA(Trp).2. The protein should have ATP binding and be active in cytoplasm.3. The protein should be able to bind L-tryptophan ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "ILGWF",
    "response#2": "MISH",
    "response#3": "LKII"
  },
  {
    "reference": "MNESNCTKIVNFLKGMRLKTNPNLPGAKQKTPLNIHRFYLLLIIIIYTATSACIYFDWTSIRNLLLNVGKYEHLNISKYADITLSPQYKKINNLYPMTLAIHFTMSVFCGFLYDHIGPKFTAIIGQGFNILSWIFLSIDTTKIDTTLIGFIFLGLGADTAFIPILTVSNLFPDISTFIMTVIGAAASLSYAVPATLNFVYKKYPHFPFYYICYGYIFIILIPCLLVATFLLPMKPFKGLDYYLENDQESDSKNKEQISYTDNDVEMQPSLIQNGNTNVSNNVNKNKATKNIIEGENFHKQSILLFFKVLLSYPSICIIVYFILFNISTVFYGMVTDIYFSYNKSIINIINILMPISFIPCIIFGRFINKYGAAIIIIIMNAFSALMHLTALIKHQAAGLISAFLYMCAASIYTSQIYCFLLNAFPSVVFGKLLGITSLFGGMFSLFCEKLYDNISNSSGNKNDPTTISILLAISFIIMFLPLSILYTRNYEKNIESVNSEKNQIQA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have L-arginine transmembrane transporter activity to facilitate L-lysine import across plasma membrane.2. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "HMQMISIGGAIGTGLYVGSGSSLADGGPASVIINYSLIGIMMFFIVYALGEMAVAYPVAGGFNTYATRFIDPAWGFAVSWNYFINYFVTFPLELTTCAITFRYWTDINSAAWISIFLVVIIIVNLFGVRAYGEVEFILSTVKVVATFGFIILAIIINCGGVPTDHRGYIGGSIIKHKPFRHGFKGFCSVFTTAAFSFSGTEVIGLAAAEVDNPQKALPHAVKQVFWRIAIFYVVSLILIGLLISPDDPNLMGNGSTSVSPFVLAIKEANIKGLPSVFNAVIIISVVSVTNSSTYTAGRTLHGMANLKQAPSFFKYTDRLGRPLLAMIVVLLFGFFAYINEADKNGNDVSDTVFNWLLALSGLSNFFTWGSICLCHIIFRLAFKKQGHSLKELGFVSPMGIWGSCIGLFFNILCLMAQFYVSLFPIGGKPNANDFFQGYLAAPVTLAFFIGYKIYDRSHIPSL",
    "response#2": "HMQMISIGGAIGTGLYVGSGSSLADGGPASVIINYSLIGIMMFFIVYALGEMAVAYPVAGGFNTYATRFIDPAWGFAVSWNYFINYFVTFPLELTTCAITFRYWTDINSAAWISIFLVVIIIVNLFGVRAYGEVEFILSTVKVVATFGFIILAIIINCGGVPTDHRGYIGGSIIKHKPFRHGFKGFCSVFTTAAFSFSGTEVIGLAAAEVDNPQKALPHAVKQVFWRIAIFYVVSLILIGLLISPDDPNLMGNGSTSVSPFVLAIKEANIKGLPSVFNAVIIISVVSVTNSSTYTAGRTLHGMANLKQAPSFFKYTDRLGRPLLAMIVVLLFGFFAYINEADKNGNDVSDTVFNWLLALSGLSNFFTWGSICLCHIIFRLAFKKQGHSLKELGFVSPMGIWGSCIGLFFNILCLMAQFYVSLFPIGGKPNANDFFQGYLAAPVTLAFFIGYKIYDRSHIPSH",
    "response#3": "LSMMQTLIHLLKCNIGTGLLGLPLAIKNAGLLVGPVSLLAIGVLTVHCMVILLNCAQHLSQRLQKTFVNYGEATMYGLETCPNTWLRAHAVWGRYTVSFLLVITQLGFCSVYFMFMADNLQQMVEKAHVTSNICQPREILTLTPILDIRFYMLIILPFLILLVFIQNLKVLSVFSTLANITTLGSMALIFEYIMEGIPYPSNLPLMANWKTFLLFFGTAIFTFEGVGMVLPLKNQMKHPQQFSFVLYLGMSIVIILYILLGTLGYMKFGSDTQASITLNLPNCWLYQSVKLMYSIGIFFTYALQFHVPAEIIIPFAISQVSESWALFVDLSVRSALVCLTCVSAILIPRLDLVISLVGSVSSSALALIIPALLEIVIFYSEDMSCVTIAKDIMISIVGLLGCIFGTYQAL"
  },
  {
    "reference": "MALFGFGKKLALPTPEKALPGRAQIMPVPANHYVNKNPLKPPFPDGFEKALFGLGCFWGAERKFWQQQGVYSTAVGYAAGFTPNPTYDEVCTGLTGHNEVVLVVFDPKVISYTQLLKVFWESHNPTQGMRQGNDVGTQYRSGIYVYSEAQKQLAEASRDAYQQALSSAGYEKITTEILDAPEFYYAEAYHQQYLAKNPNGYCGLGGTNVACPVGVFESSANG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein is the optimization of the active site to achieve high catalytic efficiency for [thioredoxin]-disulfide + H2O + L-methionine = [thioredoxin]-   dithiol + L-methionine (S)-S-oxide.2. The protein design must be able to perform peptide-methionine (S)-S-oxide reductase activity, L-methionine:thioredoxin-disulfide S-oxidoreductase activity in order to contribute to protein modification process.3. The protein must exhibit the following characteristics: Has an important function as a repair enzyme for proteins that have been inactivated by oxidation; Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine .. The designed protein sequence is ",
    "response#1": "MEIAVLGLGCFWGPEIKFSKLEGVIKTEVGYCGGDSKTVTYKEVCTCNTNHAEVVKLDFDPKIISYEKILNFFFEIHDPTTLNSQGPDFGTQYRSEIFYLSDRQKEIAESVIKKVNLKLSGNVVTKSSLLKNYCPAEEYHQRYLEKR",
    "response#2": "MEIAVLGLGCFWGPEIKFSKLEGVIKTEVGYCGGDSKTVTYKEVCTCNTNHAEVVKLDFDPKIISYEKILNFFFEIHDPTTLNSQGPDFGTQYRSEIFYLSDRQKEIAESVIKKVNLKLSGNVVTKSSLLKNYCPAEEYHQRYLEKQ",
    "response#3": "MQQAVFGLGCFWGAERRFWQQPGVFSTAVGYAGGFTPNPTYEEVCSGLTGHTEVVLVVFDPQQTSFDALLKVFWEAHNPTQGMRQGNDQGTQYRSAIYCQDHAQLEAALASQARFQAELDKAGLGSITTEICEAPIFYYAETYHQQYLAKNPGGYCGLGGTG"
  },
  {
    "reference": "MDSRMEIDFMDLNSKPKLSEMEKQHKKVSGMKWPFSLADLATHHEHTFFQNYKSTPIVSINSKNSSLNNYKSTIDPQYFRGTFPLLAKTSTYDSRKNYDNLSPNESTLTIFYMGEVHIFPGISPEKAELIIDLVSKSTTLHMDEILEKVMNKEKYEENKSDPSNASTNYAKGALAMARRATLARFLEKRKHRLIKARPYLYGENLSKFPFDIQQQEEETASSSVHWEN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should contain one or more Tify domains that are essential for its function. The designed protein sequence is ",
    "response#1": "VRRRVYTRRHNRDQHDGGDTLCALIYCNQNHTARVLFELLAEMGKRDADLKFLRCQYTTDRVADPTTEPKEAELEHRRQEEVLKRFRMHDCNVLIGTSVLEEGIDVPKCNLVVRWDPPTTYRSYVQCKGRARAAPAYHVILVAPRYTSPAIGVVQLTDKSHRFICGAGGGAADSTEVDSDSDDSAMPNSLGSDPYTFGTARGTVKILNPEVFSSVPPMACEIC",
    "response#2": "ELVANYTGLDKIYEMVDQDRNAIWERRNDVLRELPEQLPILLHCVYWKERDDVADIWYLLKRWPLISVERSLELLDYAYPDPAVRRFAIKCLQILKDDDLLLYLLQLVQAIKHESYLESDLVVLLLERALRNQRIGHYFFWHLRSEMQTPSMQTRFGLLLEVYLKGCKHHVC",
    "response#3": "AEPEKPLKDLKELVANYTGLDKIYEMVDQDRNAIWERRNDVLRELPEQLPILLHCVYWKERDDVADIWYLLKRWPLISVERSLELLDYAYPDPAVRRFAIKCLQILKDDDLLLYLLQLVQAIKHESYLESDLVVLLLERALRNQRIGHYFFWHLRSEMQTPSMQTRFGLLLEVYLKGCKHHVAPLRR"
  },
  {
    "reference": "MKGYHFHYFICNKGIIMKKSKLVLILTALLSCAAIPTSVMAENHVQPTMEKSNMSEATKDLHEIYLAGGCFWGIEAYMERIYGVEDAVSGYANGKGDKTSYQMIGITDHAETVKVTYDRNKISLDKLLKYYFQVIDPTSVNQQGNDRGRQYRTGIYYQDAADKPLIEKELAALQEKYKNKIQVEVEPLRNYVLAEDYHQDYLKKNPNGYCHIDLEQANNVIIDEKDYPKPSDAELKAKLTPLQYSVTQNKNTEHSFSNEYWDNFKPGIYVDVTTGEPLFSSKDKFESGCGWPSFTKPIAKEVVTYQDDHSFNMLRTEVISRSGKAHLGHVFDDGPKDKGGLRYCINSASIKFIPLEDMAKENYGYLINAVKE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be designed to catalyze the following reaction [thioredoxin]-disulfide + H2O + L-methionyl-[protein] =   [thioredoxin]-dithiol + L-methionyl-(S)-S-oxide-[protein].2. For general function, the protein need meet that Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.3. The designed protein should be optimized for peptide-methionine (S)-S-oxide reductase activity, L-methionine:thioredoxin-disulfide S-oxidoreductase activity, peptide-methionine (R)-S-oxide reductase activity activity in order to improve protein modification process, protein repair.4. Design the protein with a signal peptide incorporated.5. The designed protein must have at least one MsrB domain. The designed protein sequence is ",
    "response#1": "MQQAVFGLGCFWGAERRFWQQPGVFSTAVGYAGGFTPNPTYEEVCSGLTGHTEVVLVVFDPQQTSFDALLKVFWEAHNPTQGMRQGNDQGTQYRSAIYCQDHAQLEAALASQARFQAELDKAGLGSITTEICEAPIFYYAETYHQQYLAKNPGGYCGLGGTGHFAGL",
    "response#2": "METAVIGGGCFWCVEAVLRELNGVASIRPGYAGGHTANPTYKEVCTGTTFHAEVVEVTFDPAILSYVDLLRIFLTIHDPTTPNRQGADVGTQYRSVIMPASTEQENAARGVLAEVEAAQIWQAPLSTTIEPLQIFYPAEPEHVDYFARHPEAAYCQAVIEAAADEVSLTYDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEPLFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGPQDKGGLRYCINSLSIRFIPPSQEEILDTLTTEQFRVTQQKDTERPFSSEYDTLDEPGIYVDIVTGQPLFSSDDKYDAGCGWPSFTRPMVGDALHYEEDFSHGMHRVEVTSSRGESHLGHVFPDGPHAEGGQRYCINGASLRFVPLAQMEAEGAEAIRDKLTAEQYKVTQESGTETPFANEFWDKFEKGIYVDIVTGEPLFSSTDKFESGCGWPAFSKPIEGPSVVELEDNSHGMRRTEVRSRAGDSHLGHVFTGDPESPNGVRYCINSASLRFVPDN",
    "response#3": "LSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEPLFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGPQDKGGLRYCINSLSIRFISKSELKEKLTAEQYAVTQEKATERPFSSKYDKFEERGIYVDVVSGKPLFSSSDKYDAGCGWPSFTKPITADALKYEQDNSHGMSRVEVTSKISDSHLGHVFDDGPSERGGLRYCINGASLKFIPEE"
  },
  {
    "reference": "MKLLTAAQMRQLDQQTIAEIGIPSVVLMENAGRTTYQILRREFPELGEPVVVLAGRGNNGGDGFVVSRYLANAGFEVVVLLLAEKSQIGGDARINLEVLEHLSVPVREVTSAAALQEQVPLLKSAALLVDAILGTGLNSDVKGLYRDAVVLINEVATPVLAIDIPSGLSADTGQPLGMAVAADVTVTYGFPKIGQILPPGRHLVGRLWQVDISIPPDLAEISVMELSQASGLRQFLPRRPVDSHKGTFGHLFVLAGSVGKTGAAALCGEAALRMGAGLVTVGVPASLNPILEVKLTEAMTLPLTEVQGVQALGKRALEEIAADWPGKTALAVGPGLGRHPETVELVRQLVRRSTLPVVVDADGLNALAHDVDSIREAAAPVILTPHPGEMSRLLGATTKDIQADRLGAARELAERSQAIVVLKGAQTIVASPMGKVMINTTGNPALAQGGAGDVLTGMIGGLLAQKVDPFNAACLAIYLHGLTADVQAAWQGDCGLLASELLDQIPHVIKEFASGALPHIEEHICYRLVIS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..2. The designed protein must possess ATP binding, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, metal ion binding to support nicotinamide nucleotide metabolic process under certain conditions.3. The presence of Mg(2+) is necessary for the protein to function in the desired environment.4. The (6S)-NADPHX, K(+), AMP binding site should be located in a region of the protein that is accessible to the ligand.5. The desired protein should be able to perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate The designed protein sequence is ",
    "response#1": "MQIICRNCEEMGISSLQLMENAGAAATNMILKSFPNSKKVIICCGTGYNGGVGMVVARHLAPHQKKVIVAIVKKDGLIESDITLRNYQILQEMECSIELINISESTVRNFKRLLTDADLIVDALFDLGFSGTLSEFSRRVIQSINATNKKVLCLDLPSGMISDNKKKPKTMIKADLIVTFHDTKLCLTRKELKEKTVICNIGIPPEAQVLVIGGDYGMAGAAALAAEAALRCGAGLVRVATRPQHVAALVARTPEIMPVGVESGADLQPLLDSADTLVVGPGLGQSPWSEYLLQAALASGKPMVLDADGLNLLAAGKVQPRPRMIVTPHPGEAARLLGVSTAEVQADRFAATSALQQKLGGVVVLKGNGSLIASEKEMLLSDYGNPGMASGGMGDVLSGVIGALLAQGQEPLAAAALGVCLHGAAADIAAEEGMRGLVATDLVGIVAGSARYPGAAILASGAAVSATSGMTRYVGSAADQVVSHYPEVVAANGLADAGKVQAWAVGPGMGTGDEALEILRTVLATDLPVLVDADGLTVLAQNLDLVRGRSAPTLLTPHAGEFARLAGTGVGDDRLSAVRALAAELGATVLLKGRITLVATPTGRVYGNDAGSSWAATAGAGDVLTGIAGSLLAAGLEPALAGACAARVHALAAARAAAGAPL",
    "response#2": "MAQADRAAVALGHPVAELMENAGRAVARAVRARFAPCRTLVLCGPGNNGGDGYAAARLLDLWGWPVRVAALAPPRGPAVAVAARWRGPVVPFTPAETARCPLVIDAVFGAGLARDLDPAVAEVLRAAPRLVAVDVPSGLDGATGQPRGFAPRAVLTVTFFRRKPGHLLLPGRTLCGETVLADIGIPAAALEPADYADVVAPHTNNVDKYSRGSVLVVAGSSRYPGAAVMAAKAAARAGAGYVTLAVPVTIAPLIQMQLPEVPVVGVAAEQDGTFSAAARPVILKLAERSSATLVGPGMRVTGGTVAVVSALINSKAPLVVDADGLNCLARLTNGHLDEFPEVTRRDAPLVLTPHRRELGRLVGLADTPPDSLTAALEAARRIVWADGGSELCIVAKGTATACVGVEAALLPEPGTASLATAGSGDVLGGIICALLARMPDDIEDLPLIAAFGCEVHAVAGSRAAKRYGSRGVMAGDIADEVGLAVDEVLGA",
    "response#3": "MQIICRNCEEMGISSLQLMENAGAAATNMILKSFPNSKKVIICCGTGYNGGVGMVVARHLAPHQKKVIVAIVKKDGLIESDITLRNYQILQEMECSIELINISESTVRNFKRLLTDADLIVDALFDLGFSGTLSEFSRRVIQSINATNKKVLCLDLPSGMISDNKKKPKTMIKADLIVTFHDTKLCLTRKELKEKTVICNIGIPPEAQLKRRFFENRKGDFGKLLVIGGSDKYHGAPILSAMSSIRAGVDLVIISTPELIANTIRAHTPNFIVRDYPGRYLTLDSVKYISDLINWADAIVLGPGLGVESETKEAVCKLFTLIKEANKPLVVDADALKVLAENPRILAGSPAVATPHRGEFKLLTGLEIKSSADILDNINEILSKISEFDITFAVKGAVDLVAYKNMFKLDLLGNPGMTVGGIGDVLTGVLGCFLSQGVEPFKAACAAVFITGLSGNLIAEKKGYHFTSAELIEYIPEANVDKYSRGSVLVVAGSSRYPGAAVMAAKAAARAGAGYVTLAVPVTIAPLIQMQLPEVPVVGVAAEQDGTFSAAARPVILKLAERSSATLVGPGMRVTGGTVAVVSALINSKAPLVVDADGLNCLARLTNGHLDEFPEVTRRDAPLVLTPHRRELGRLVGLADTPPDSLTAALEAARRIVWADGGSELCIVAKGTATACVGVEAALLPEPGTASLATAGSGDVLGGIICALLARMPDDIEDLPLIAAFGCEVHAVAGSRAAKRYGSRGVMAGDIAGPGDVITSLKRRFFENRKGDFGKLLVIGGSDKYHGAPILSAMSSIRAGVDLVIISTPELIANTIRAHTPNFIVRDYPGRYLTLDSVKYISDLINWADAIVLGPGLGVESETKEAVCKLFTLIKEANKPLVVDADALKVLAENPRILAGSPAVATPHRGEFKLLTGLEIKSSADILDNINEILSKISEFDITFAVKGAVDLVAYKNMFKLDLLGNPGMTVGGIGDVLTGVLGCFLSQGVEPFKAACAAVFITGLSGNLIAEKKGYHFTSAELIEYIPEALEE"
  },
  {
    "reference": "MSINAEEISTLIKQQIENFDSDIEVSDVGTVIEVGDGIARAHGLDNAMAGELLEFSNGVMGLAQNLEESNVGIVILGPYTEIKEGDEVRRTGRIMQVPVGEELLGRVVNPLGQPIDGKGPIETSKTRPIEAAAPGVMDRKSVDEPLQTGIKAIDALVPIGRGQRELIIGDRQTGKTTVAVDTILNQKDQDMICIYVAIGQKESTVRNTVETFRKHGALENTIVVTAGASDPAPLLYLSPYAGVAMGEEFMYNGKHVLVIYDDLSKQAVAYRELSLLLRRPPGREAFPGDVFYLHSRLLERAAKLSDAKGGGSLTALPFVETQAGDIAAYIPTNVISITDGQIFLQSDLFFSGVRPAINPGLSVSRVGGSAQIKAMKKVAGTLRLDLASFRELEAFSQFGSDLDKSTQAKLNRGQRTVEVLKQGLHKPMVVEKQVAIIYSLTRGFLDDIPVEDIQRFEEELNIWLESNRNELLTSIRETGNLPDESEFNDAIDSFKKTFLPSNN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.2. The protein should have a fast turnover rate for ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate.3. The protein can be targeted to proton-transporting ATP synthase complex, catalytic core F(1), plasma membrane and perform ATP binding, proton-transporting ATPase activity, rotational mechanism, proton-transporting ATP synthase activity, rotational mechanism.4. The protein must exhibit the following characteristics: Produces ATP from ADP in the presence of a proton gradient across the membrane; The alpha chain is a regulatory subunit.. The designed protein sequence is ",
    "response#1": "KGGKIGFFGGAGVGKTVLVMELIRNIAIEHHGFSMFAGVGERTREGNELYLDMQEAEVLDNTVLVFGQMNEPPGARFRVALSALTMAEYFRDVEGRDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLATDMGELQERITSTKKGSITSVQAIYVPADDITDPAPATTFTHLDATVVLSRRGPAIDVTRSVDHLKGGKIGFFGGAGVGKTVLVMELIRNIAIEHHGFSMFAGVGERTREGNELYLDMQEAEVLDNTVLVFGQMNEPPGARFRVALSALTMAEYFRDVEGRDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLATDMGELQERITSTKKGSITSVQAIYVPADDITDPAPATTFTHLDATVVLSRRG",
    "response#2": "MKKVAGTLKIDQAQFRELESFSKFGGEMDAVTAFTIDKGQKNTQLLIQPQYSPMPVEEQISILYCGTQGLLKEVPLDKVHEFEARFLQELRTSHQRDVLDVLKTGVINDEIRGILEKTAKETPAAAAAS",
    "response#3": "MKKVAGTLKIDQAQFRELESFSKFGGEMDAVTAFTIDKGQKNTQLLIQPQYSPMPVEEQISILYCGTQGLLKEVPLDKVHEFEARFLQELRTSHQRDVLDVLKTGVINDEIRGILEKTAKEA"
  },
  {
    "reference": "MTDVAKTLEELCTERGMRMTEQRRVIARILEDSEDHPDVEELYRRSVKVDAKISISTVYRTVKLFEDAGIIARHDFRDGRSRYETVPEEHHDHLIDLKTGTVIEFRSPEIEALQERIAREHGFRLVDHRLELYGVPLKKEDL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be optimized for DNA binding. The designed protein sequence is ",
    "response#1": "LPNRVIKQILEQEKLPHLKGKYNKVRSEVPYGKDKKSRIDFVLTNESQQNPIYLEVKNTTLAKDKIALFPDTVTTRGQKHLQELMDLLPDAQPIMLYFINRGDCQQFSPGDDYDPGYGKLFREAVKKGVEILPCRFEITPS",
    "response#2": "IPNKVIKYALENQWFAQLKERYTTVRSEVPYGKDNKSRVDFLLTQNEGKSPIYVEVKNTTLCQGEIALFPDTVTSRGQKHLRELMALLPDTKPIMLYFINRGDCYSFAPGDEFDPTYGTLLREAVKKGLEVLPCRFEVTPEG",
    "response#3": "IPNKVIKYALENQWFAQLKERYTTVRSEVPYGKDNKSRVDFLLTQNEGKSPIYVEVKNTTLCQGEIALFPDTVTSRGQKHLRELMALLPDTKPIMLYFINRGDCYSFAPGDEFDPTYGTLLREAVKKGLEVLPCRFEVTPEL"
  },
  {
    "reference": "MIFIFISFLSLFFKWQRLMFILISLEFIVMSLFILFSGDLNEMMFFYFMCFSVVSSVLGMVVMVGNVKFYGSDLCLF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be optimized for mitochondrial membrane, respirasome-specific folding and stability, while maintaining NADH dehydrogenase (ubiquinone) activity.2. For general function, the protein need meet that The immediate electron acceptor for the enzyme is believed to be ubiquinone .3. The protein should have a low Km value for a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +   NAD(+). The designed protein sequence is ",
    "response#1": "DVGAMTPFLWGFEEREKLMEFYERVSGARMHAAYIRPGGVSQDMPMGLSEDIYKFAKQFGSRIDEIEDVLSSNRIWKQRLVDIGTVSAEEALDWGFSGVMLRGSGIAWDLRKTQPYDVYDELEFDIPVGTKGDCYDRYLIRVEEMRQSLKLIMQCLNKMPTGVIKVDDKKISPPSRVDMKDSMEALIHHFKLYTEGYSVPIGETYTAVEAPKGEFGVYLVSDGSSKPYRCKIKAPGFSHLQGLNFMSKGHMIADVVTIIGTQDIVFGEVDR",
    "response#2": "SSHWFMTWLGFEMNMMAIVPVLMKKYSPRSMEAATKYFLTQATASMILMLAIIINLMYSGQWTIANMENHTASMLITIALVMKLGLAPFHFWVPEVTQGIPLSSGLILLTWQKIAPLSLLYQIHSSINMELFLTMSLLSIVIGGWGGLNQTQLRKIMAYSSIGHMGWMMAIMNYNPNLSFLNLLVYILMTSSMFALLIFSSTTSTLSLSLAWNKTPIIATMSLIILLSLGGLPPLTGFMPKWMIIQELTKNNSTILPTLMAISALFSNYFFN",
    "response#3": "STHWFMIWLGFEMNMMAMIPVLMKKYSPRAAEAATKYFLTQATASMILMLALIINLMYSGQWTIMNMTNNMASLLITIALTMKLGLAPFHFWVPEVTQGVSLQAGLILLTWQKIAPLAVMYQIFASINPNLLLTMALLSIMIGGWGGLNQTQLRKIMAYSSIAHMGWMTAIMIYNPNLMLLNLLLYILMTTSMFMMFMYNSTTTTLSLSLTWNKTPLMTVLMMTTLLSLGGLPPLTGFMPKWMIIHELTKNNSVILPTM"
  },
  {
    "reference": "MDSFMLQDEGVRDRIRQAEEFLDPNDPQVRSYRSDIILMLQKNQRRLVVNLDHVRNHNQELAQGLLQQPFDFTLAFDQALKNIVQTIPQARPDQTAKDTIYYCAWAGSFGLNSCNPRTLSSHLLNYMVSIEGIVTRTSLIRPKVVKSVHYNEKKDMFHFREYQDQTMTNGVTTSSVYPREDDDGNPLITEYGFCTYRDHQTISIQEMPERAPAGQLPRGVDAILDDDLVDSVKPGDRVQLVGIYRTLGNRNTNHNSALFKTMILTNNVVLLSSKSGGGVATATITDTDIRNINKVSKKKNLLELLSQSLAPSIYGHDYVKKAILLMLLGGIEKNLENGTHLRGDINILMVGDPSTAKSQLLRFVLNTAPLAIATTGRGSSGVGLTAAVTSDKETGERRLEAGAMVMADRGVVCIDEFDKMSDVDRVAIHEVMEQQTVTIAKAGIHTSLNARCSVVAAANPVFGQYDPHKDPHKNIALPDSLLSRFDLLFVVTDDIEDTRDRHVSEHVLRMHRYRQPGTEEGAPVREQGTQSLGVSASNQNESQGPTEVYQKFDAMLHSGVTITSGRGANKKPEILSIPFMKKYIQYAKTRIKPVLTQEVSDRITDIYVGLRNDEMEGNQRRTSPLTVRTLETIIRLATAHAKSRLSSRVEERDAIAAEGILRFALFKEVVEESRKKRRKTQTVDFASSSDESSSDDEDGDQANGTQASRSASRATRNSNRNQTRGTSDSREPEAPENDSQATPRRSGRRVQDSSQTSFASSMPSSQLP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have a low Km value for ATP + H2O = ADP + H(+) + phosphate.2. The designed protein must have at least one MCM domain.3. For general function, the protein need meet that The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.; Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells4. The protein should be optimized for nuclear pre-replicative complex, cytoplasm-specific folding and stability, while maintaining single-stranded DNA helicase activity, DNA replication origin binding, chromatin binding. The designed protein sequence is ",
    "response#1": "VYELLAKSIAPEIYGHLDVKKALLLLLIGGVTKEMGDGMKIRGDINICLMGDPGVAKSQLLKYISKVAPRGVYTSGRGSSGVGLTAAVMRDPVTDEMVLEGGALVLADNGICCIDEFDKMDETDRTAIHEVMEQQTISISKAGISTTLNARTSILAAANPVYGRYNPRISPVENINLPAALLSRFDILFLLLDTPTRETDEQLAKHYRLPGFA",
    "response#2": "RRMTELEQSGQLYEYLSRSIAPEIFGHLDVKKALLLQLIGGVTKEMGDGMRIRGDINVCLMGDPGVAKSQLLKYITKVAPRGVYTTGRGSSGVGLTAAVMRDPVTDEMVLEGGALVLADNGTCCIDEFDKMDDSDRTAIHEVMEQQTISISKAGITTTLNARTAILAAANPLYGRYNPRISPVENINLPAALLSRFDVLFLILDTPNRDSDEELARHVTFNMRDLNPGDIDKLISIKGLVLRCTPVIPDMKQAFFRCSVCGHCVTVEIDRGRIAEPIKCPREVCGATNAMQLIHNRSEFADKQVIKLQETPDVVPDGQTPHSVSLCVYDELVDSARAGDRIEVTGIFRCVVQIRDLGSTHVSRLVRVPGIIIGSGSVSNKVKTVTLICSHCKDQIKIEVTPGFASLNIPRACQGPPNPNGEAKNCPLDPYKILHEKSEFVDQQVLKMQEAPEMVPVGEMPRHVIICADGYLANRVVPGTRIMAIGVYAIEK",
    "response#3": "QAAFESFLSEYKAPEQTIAHALGNITIDENDFSDDELMEDDDTRETRRQAKAPPGPKYKVMLQQLADRKTDEVMIELDDLENFETQLGDELKLVQSIEMNAKHYVELFSRAVDNLLPEPSVGVTFKDDVLDVLMERRRQMNERLERRHAREAAERTLSSHYLNHMVSLEGIVTKCSLVRPKVVKSVHWNEKKSTFHFREYRDQTMTANGAASTSVYPTEDGEGNPLVTEYGYCTYRDHQTVSIQEMPERAPAGQLPRGVDVIMDDDMVDRVKPGDRIQLVGIYRRVSPEEVEQLYEYLSRSIAPEIFGHLDVKKALLLQLIGGVTKEMGDGMRIRGDINVCLMGDPGVAKSQLLKYITKVAPRGVYTTGRGSSGVGLTAAVMRDPVTDEMVLEGGALVLADNGTCCIDEFDKMDDSDRTAIHEVMEQQTISISKAGITTTLNARTAILAAANPLYGRYNPRISPVENINLPAALLSRFDVLFLILDTPNRDSDEELARHVRQVRGEHLGHLITIRAIATRVSDVKPIVQVSAYTCDRCGCEIFQPITDKQYGPLTMCPSEDCKQNQSKGQLNPSSRASKFLPFQEVKVQEMAEQVPIGQIPRSLTVFCYGTLVRQISPGDVVDISGIFLPVRQVRGEHLGHLITIRAIATRVSDVKPIVQVSAYTCDRCGCEIFQPITDKQYGPLTMCPSEDCKQNQSKGQLNPSSRASKFLPFQEVKVQEMAEQVPIGQIPRSLTVFCYGTLVRQISPGDVVDISGIFLP"
  },
  {
    "reference": "MARFSLVVVVTLSLAISMFPDTTTAQLKTNFYGNSCPNVEQIVKKVVQEKIKQTFVTIPATLRLFFHDCFVNGCDASVMIQSTPTNKAEKDHPDNISLAGDGFDVVIKAKKALDAIPSCKNKVSCADILALATRDVVVAAKGPSYAVELGRFDGLVSTAASVNGNLPGPNNKVTELNKLFAKNKLTQEDMIALSAAHTLGFAHCGKVFNRIYNFNLTHAVDPTLNKAYAKELQLACPKTVDPRIAINMDPTTPRQFDNIYFKNLQQGKGLFTSDQVLFTDGRSKPTVNDWAKNSVAFNKAFVTAMTKLGRVGVKTRRNGNIRRDCGAFN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.2. The designed protein should be optimized for metal ion binding, lactoperoxidase activity activity in order to improve hydrogen peroxide catabolic process, response to oxidative stress.3. The designed protein should have a binding site that is specific to the target substrate, heme b ligand. The designed protein sequence is ",
    "response#1": "GFTVGILASTSSSTSISQAAQLATSFSSRGIRAVIVGESLLSGTDQTYSSADATAFDAVVVTMGAETLFGPVAKPNTLFPSGRPSQILHDAYRWGKPVGAVSKASVVLEPLPGTKNQGGVYRVESVNELATSIAKGLETFRFVDRFPLG",
    "response#2": "GFTVGILASTSSSTSISQAAQLATSFSSRGIRAVIVGESLLSGTDQTYSSADATAFDAVVVTMGAETLFGPVAKPNTLFPSGRPSQILHDAYRWGKPVGAVSKASVVLEPLPGTKNQGGVYRVESVNELATSIAKGLETFRFVDRFKK",
    "response#3": "AFARAWFKLTHRDMGPRARYLGADAPKEDLIWQDPVPAVNHPLVTPADVTALKGQIQASGLSTGELVRTAWAAAGSFRGTDLRGGTNGGRVRLAPQKDWAVNNPAELARVLPVYERIATGFNTSRKDGRKISLADLIVLGGAVGVEQAAKAAGVTVEVPFTPGRMDAAQAQTDVNSFAVLEPKADGFRNYFGEGNRLSPAEMLVERANLLTLTVPEMTVLVGGMRVLGGNSGGAAHGVFTDTPGRLDNAFFVNLLDMGTKWAKSASGEGLYEGTDRATGKVKWTATPVDLVFGSNSELRAVSEVYAAQ"
  },
  {
    "reference": "MGHAENKQSWRTSCTELLISVATPGCNGFPNLESKENYKLNISRDSMLLYADAVWGALRGLETFSQLVGRDENGMYYINETEIVDFPRFPHRGLLLDTSRHYLPLKAILETLDVMAYNKLNVFHWHIVDDPSFPYESFTFPELSKQGAFSAMTHVYTASDVQTVIEYARLRGIRVIAEFDTPGHTLSWGPGAPGLLTPCYLGKDPSGTYGPINPIFNSTYQFVTSLFQEISSVFPDYFIHLGGDEVDFTCWKSNPDILVFMKKMGFGEDYTKLESYYIQRLLDIVSSLGKGYMVWQEVFDNGVKVRPDTIIHVWKNNLPYAEEMANVTKAGYRALLSAPWYLNRISYGQDWMAAYQVEPLKFTGSTKQKDLVIGGEACMWGEYVDVTNLTPRLWPRAGAVAERLWSNATVRNLQDAYVRLADFRCELLRRGVQAEPLFTGYCDHEFGGF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The design of the protein must focus on the precise arrangement of amino acids in the Proton donor to enable effective catalysis of ganglioside GM2 + H2O = ganglioside GM3 + N-acetyl-beta-D-   galactosamine.2. The protein should have a specific function in ganglioside catabolic process, neuromuscular process controlling posture, carbohydrate metabolic process, lysosome organization and should be localized to lysosome, membrane in order to carry out this function efficiently.3. The designed protein must have at least one Glycoside hydrolase family 20 catalytic, Beta-hexosaminidase eukaryotic type N-terminal domain. The designed protein sequence is ",
    "response#1": "YDDSGPAWVDANHMTTQNPRTPDTLPVLYADEYGFHNGIRLWRGSFTDAASGVYLNVQGGAAFGWSAYLNGHFLGSL",
    "response#2": "FLHRGILIDTARHYLPVKTILKTLDAMAFNKFNVLHWHIVDDQSFPYQSITFPQLSNKGSYTLSHVYTPHDVRKVTEYARLRGIRVIPEFDTPGHTLSWGKGQKDLLTQCYSGKIPTDSDGPINPMLNTTYMFLTMLFQEISKVFPDQFIHLGGDEVEFNCWASNPDIQNFMKQKGFGQDFTKLESFYMQKLLDIIASMKKRSIVWQEVFDDKSELLPGTVIQVWKIRDYASKLSQVTASGFPAILSAPWYLDWISYGQDWRKYYLVEPLDFQGSDKQKKLVLGGEACLWGEYVDATNLTPRLWPRASAVGERLWSPEFYTQALQAMVDFVEANRRGG",
    "response#3": "FAHRGLLLDTSRHYLPVRAILETLDVMAYNKLNVFHWHIVDDPSFPYESTAFPELSRKGAYNPATHVYTANDVKLVIEYARLRGIRVISEFDTPGHTHSWGPGAPGLLTPCYAGSVPSGSYGPVNPTLNATYWFMNELFREVSSVFPDFYLHLGGDEVDFTCWRSNPEVQAFMQKMGFGQDYTRLESFYIQRLLDIVSSYGKGYVVWQGGFDNGVRVKPDTIIHVWRENAGAYQQEMARVTKAGYQALLSAPWYLNRISYGQDWQAAYEVEPLAFEGSPEQRALVIGGEACMWGEYVDVTNLTPRLWPRAGAVAERLWSAPDCVGDQALFAHRGLLLDTSRHYLPVRAILETLDVMAYNKLNVFHWHIVDDPSFPYESTAFPELSRKGAYNPATHVYTANDVKLVIEYARLRGIRVISEFDTPGHTHSWGPGAPGLLTPCYAGSVPSGSYGPVNPTLNATYWFMNELFREVSSVFPDFYLHLGGDEVDFTCWRSNPEVQAFMQKMGFGQDYTRLESFYIQRLLDIVSSYGKGYVVWQGGFDNGVRVKPDTIIHVWRENAGAYQQEMARVTKAGYQALLSAPWYLNRISYGQDWQAAYEVEPLAFEGSPEQRALVIGGEACMWGEYVDVTNLTPRLWPRAGAVAERLWSDPQRHPFGYDAAHWAPPLTDDPFAPETDLVLVQGPYLVRSASLSGSTLALRGDSANETALEVFASKKVHTVTWNGKRIKTSRSSYGSLP"
  },
  {
    "reference": "MSVQSSLTIYAEQDPQTILEHTVDGDRIAELLAGVGIKFERWEANKTLADDADQDAVLAAYADDVARIKEENGFQSVDVVRLKAGHPDRKAFRQKFLAEHIHAEDEVRFFVEGSGWFYLHLDDKIYVTQCVQNDFIHVPAGTKHWFDMGNQPEFAAIRFFTNPEGWVAQWTDNPIALNFPMYVHAEPVKAVVTDIEGTTSSISFVKDVLFPYAKEHLAAFVAAQGDAPQVAAILDQAREESGQPDADNATIAQTMLRWIDEDRKVTPLKAIQGLIWEKGYRDGDFQAHLYDDAHAALKQWADSGLALYIFSSGSIKAQHLMFEHTTFGDLRPWFKGYFDTTTGPKKEAESYRKIAAEIGVDPHEILFLSDVVAELDGARAAGMQTRWLVRDGQQPAHPFHTQTDTFATIQPGAAVS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have a high specificity for Ni(2+) binding, with minimal off-target effects.2. A protein that is capable of catalyzing reactions within the Amino-acid biosynthesis pathway with high efficiency and specificity.3. The designed protein must be able to perform acireductone dioxygenase (Ni2+-requiring) activity, iron ion binding under specific conditions. The designed protein sequence is ",
    "response#1": "PRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGGLLGVRDEMVLGVKDCAGPRSAAEMSEAEREALGIRERMPLGCEEGRNALEGDTELKGVLGEEFVERYLAVNRTLETVLVDKKETEEQRLTRWLRSMRAYYFDNLPGDQRAPHDYVPSRPVDASALAKMNVLSWHHL",
    "response#2": "PRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGGLLGVRDEMVLGVKDCAGPRSAAEMSEAEREALGIRERMPLGCEEGRNALEGDTELKGVLGEEFVERYLAVNRTLETVLVDKKETEEQRLTRWLRSMRAYYFDNLPGDQRAPHDYVPSRPVDASALAKMNVLSWHGLCPRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGCGHAVTT",
    "response#3": "PRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGGLLGVRDEMVLGVKDCAGPRSAAEMSEAEREALGIRERMPLGCEEGRNALEGDTELKGVLGEEFVERYLAVNRTLETVLVDKKETEEQRLTRWLRSMRAYYFDNLPGDQRAPHDYVPSRPVDASALAKMNVLSWHGLCPRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGE"
  },
  {
    "reference": "MATVKELKATARPSVGKGAARAERRAGRVPAVIYGDNKPPVTISLEDRELRLRILAGRFLTTVVDLDLDGTKHRVIPRDFHLDPVRDFPVHVDFLRLGEGATIRVSVPLHLKNSETAPGVKRGGTVNIVTHTVDLEAEADSIPQFIEADIGTLDIGTSLHLSDVVLPKGVKLVAAREDVTLVTIVPPSGFNEEQKAAAGAPAAAAPAAAAKAPAAGAKAPAAAAKAPAAPAKKK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must be able to 5S rRNA binding. The designed protein sequence is ",
    "response#1": "PKILITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSLQKGTFV",
    "response#2": "PKILITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSLQKGTFV",
    "response#3": "PKILITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSLQKGTFR"
  },
  {
    "reference": "MVHWTQEERDEISKTFQGTDMKTVVTQALDRMFKVYPWTNRYFQKRTDFRSSIHAGIVVGALQDAVKHMDDVKTLFKDLSKKHADDLHVDPGSFHLLTDCIIVELAYLRKDCFTPHIQGIWDKFFEVVIDAISKQYH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein can be targeted to hemoglobin complex and perform heme binding.2. The protein must exhibit the following characteristics: Involved in oxygen transport from gills to the various peripheral tissues.. The designed protein sequence is ",
    "response#1": "EALGRLLVVYPWTQRFFESFGDLSTPAAVFANAKVKAHGKKVLTSFGEGMNHLDNLKGTFAKLSELHCDKLHVDPENFKLLGNMLVVVLARHFGKEFDWHMHACFQKVVAGVANAR",
    "response#2": "CLARLIVVNPWSRRYFHDFGDLSSCDAICRNPKVLAHGAKVMRSIVEATKHLDNLREYYADLSVTHSLKFYVDPENFKLFSGIVIVCLALTLQTDFSCHKQLAFEKLMKGVSHAR",
    "response#3": "CLARLIVVNPWSRRYFHDFGDLSSCDAICRNPKVLAHGAKVMRSIVEATKHLDNLREYYADLSVTHSLKFYVDPENFKLFSGIVIVCLALTLQTDFSCHKQLAFEKLMKGVSHAR"
  },
  {
    "reference": "MRVLIVFFAFLLAFFQYSFWFGKNGWSDYQEAQTAVERLKDENTKLEARNNLIAAEINDLKTGVNALEERARFEREMVKSDETFYRIVPRNQ",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in plasma membrane, cell division site that is critical for FtsZ-dependent cytokinesis.2. Include a signal peptide as an essential component of the protein.3. The protein should contain a Helical membrane-spanning region.4. The protein must exhibit the following characteristics: Essential cell division protein; May link together the upstream cell division proteins, which are predominantly cytoplasmic, with the downstream cell division proteins, which are predominantly periplasmic..5. The Periplasmic, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "RWGDKAVLNKQGEVLIPDDIDEFKNLPLITGPEGQEKKLLEIMKGVYIVLKDKSMQLAEFHVNDRRAWRIKLASGLEMQLGRKAPLENMQRFLKTMDLLGEEQVAMMASVDTS",
    "response#2": "ARWGTGRLLSDGGDVFSEAEPTIHPELPLLHGPVSQSKMMMRRYNEINQLFHPANLRLKELYLTERMTWFMQFDSGLRIIVDQDQTMNKLQRLSHLAQSDLKPVWSKISAIDLN",
    "response#3": "ARWGTGRLLSDGGDVFSEAEPTIHPELPLLHGPVSQSKMMMRRYNEINQLFHPANLRLKELYLTERMTWFMQFDSGLRIIVDQDQTMNKLQRLSHLAQSDLKPVWSKISAIDLC"
  },
  {
    "reference": "MAKTHFQGNETATSGELPQVGDNLAEFNLVNTELGEVSSKDFQGRKLVLNIFPSVDTGVCATSVRKFNEAAASLENTTVLCISKDLPFALGRFCSAEGIENVTPVSAFRSTFGEDNGIVLEGSPLKGLLARSVIVVDENGKVAYTQLVDEISTEPDYDAALAALN",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Plays a role in cell protection against oxidative stress by detoxifying peroxides..2. The designed protein must be optimized for thioredoxin peroxidase activity.3. The designed protein should contain one or more Thioredoxin domains that are essential for its function.4. The protein's Cysteine sulfenic acid (-SOH) intermediate must be carefully engineered to ensure that the amino acids involved in [thioredoxin]-dithiol + a hydroperoxide = [thioredoxin]-   disulfide + an alcohol + H2O are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "MPVKKRVPFVAFRTRVRDETIGGPNPYRWEVRTTEDYFSGKRVVLFSLPGAFTPTCSTQQLPDFERLYDEFGKVGIEAVYCLSVNDAFVMNAWGKALGLEKVRLIPDGSGEFTRKMGMLVAKDNLGFGMRSWRYAAVVNDSVVEQWFEEEGC",
    "response#2": "MPVKKRVPFVAFRTRVRDETIGGPNPYRWEVRTTEDYFSGKRVVLFSLPGAFTPTCSTQQLPDFERLYDEFGKVGIEAVYCLSVNDAFVMNAWGKALGLEKVRLIPDGSGEFTRKMGMLVAKDNLGFGMRSWRYAAVVNDSVVEQWFEEEGD",
    "response#3": "MPVKKRVPFVAFRTRVRDETIGGPNPYRWEVRTTEDYFSGKRVVLFSLPGAFTPTCSTQQLPDFERLYDEFGKVGIEAVYCLSVNDAFVMNAWGKALGLEKVRLIPDGSGEFTRKMGMLVAKDNLGFGMRSWRYAAVVNDSVVEQWFEEEGF"
  },
  {
    "reference": "MPEKQDLKSMNLEELRSFFQTLNAPSYRAGQVFAWLHRGVSSFDEMTDLSLSLRESLKKTTYISSVKIVRKLTSALDGTVKYLFSLEDGECIETVLMKYSYGYSLCISTQAGCRMGCGFCASTLNGLRRLLKPSEMLDQILMASVDSHCRISHVVLMGIGEPLDNFENVLKFLHLLSCKGGLNMSLRHVSLSTCGLVSEIYRLMDYRLQLTLSVSLHAPNDVIRSTIMPVNRKWGIDALLTACRDYIKATGRRISFEYAMIDGVNDSPACARELGTRLHGMLCHVNLIPVNPVKERKYRKSRLDKMERFRSIVAKFGLPVTVRRTLGADINASCGQLRQAAQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a high specificity for S-adenosyl-L-methionine binding, with minimal off-target effects.2. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity.3. The designed protein must have a Proton acceptor, S-methylcysteine intermediate that is structurally stable and resistant to denaturation.4. The ideal protein would be one that plays a critical role in rRNA base methylation through tRNA (adenine-C2-)-methyltransferase activity, rRNA binding, metal ion binding within cytoplasm.5. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.6. The protein design should incorporate a binding pocket that specifically recognizes [4Fe-4S] cluster to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "MTACVSSQVGCSLTCKFCATGYMDRKRNLNPDEIYDQVVLLSQQAQQNYGIPLTNIVYMGMGEPLLNYANVLKSIERITAEDGLNMAARRITVSTAGIAKMIKKLGDDNVKFNLALSLHAANDTKRNEIMPINEQNSLKALAEALKYYYNKTKNAVTYEYIVFHNFNDELQDAMELAKFCKHLPCKVNIIEYNPISFADFVNAGEDKIESFAAYLRSRDRHTVCVSTQVGCPVGCAFCATGLEGFRRNLSVAEIVGQVLDITYYVRQEDPDFQVTNIVFMGMGEPLLNYDSVLKSIQLLNDAQGLNIGMRRMTISTSGVVPKIIQLAEDNPQVGLAISLHAARDEIRNSLVPMNRRYPLNQLMRACQEYTHITHRRITFEVALTAENARREEAEALARLLKGQLGHVNLIPVNPVAGTGMERPDQEKMKKFAQVLVDAGISVSSREERGTARS",
    "response#2": "YSHGYSACISSQVGCKMGCKFCASTGAGFVRNLTSGEMLDQILTIQNDTKERIGNVVIMGIGEPLDNYENVVKFLRLINHKDGVNLGARHISVSTCGLVPEVLRLAEEKIPVTLSISLHAPNDTIREKIMPVNKRYSIDKIIEACKIYTEATNRRITFEYAMINGLNDSKENALELAGRIRGMLCHVNLIPINTVSDTGFKRSSKDKIAIFKEILERYGVETTVRRELGSD",
    "response#3": "MTACVSSQVGCSLTCKFCATGYMDRKRNLNPDEIYDQVVLLSQQAQQNYGIPLTNIVYMGMGEPLLNYANVLKSIERITAEDGLNMAARRITVSTAGIAKMIKKLGDDNVKFNLALSLHAANDTKRNEIMPINEQNSLKALAEALKYYYNKTKNAVTYEYIVFHNFNDELQDAMELAKFCKHLPCKVNIIEYNPISFADFVNAGEDKIESFAAYLREPKRLTLCISTQAGCAYGCAFCATALLGFKRNLSASEIVDQIVEAGRTAGDSARITNIVFMGMGEPLANYAHTLRAIEIITDASWGLGIAPRRVTLSTVGLIPQIEKLMQETNVSLAISLHAATDELRGRLMPVNRKYPLERLIDCCRKLPLPRRKRITFEYVLLGGVNDSLADAQALCKVLGGVPSKVNVIPFNPHPGSAFERPADQTVKCFQQVLFDHGIQANVRRARGDGEDIDAACGQLKGESR"
  },
  {
    "reference": "MGFIDEVKLCLKAGDGGDGCASFRREKFVEFGGPNGGNGGKGGNIVFISDANLNTLLHFRYRRHIKADSGKNGAGRDRSGTAGKDVILKVPVGAQIIDEESEEIIVDLDKPGMEFQVAQGGKGGLGNTNFKSSTNKAPRHFTYGQPGEEKHVLLKLKVLSDVGIIGMPNAGKSKFLTRCSNSDTKVGDYPFTTVRPHLGMVKVDDSEVVIADIPGIITDAHLGVGLGHKFLKHIERCQILLHLIDVTHDDVVSAYSCIHNELELYNSDLVEKEEIVVLNKCDLLREAEILEKKNHLANYLNKEVLCLSINGDLQPILRLLSEKLKKSNSKEIDVYDPFKA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that contributes to ribosome biogenesis by functioning in cytoplasm with GTPase activity, magnesium ion binding, GTP binding.2. The designed protein should contain one or more OBG-type G domains that are essential for its function.3. The protein must bind to Mg(2+) in order to perform its enzymatic function.4. The designed protein should be able to compete with endogenous proteins or inhibitors for GTP, Mg(2+) binding.5. The protein must exhibit the following characteristics: Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control.. The designed protein sequence is ",
    "response#1": "ADVGLVGFPSVGKSTLLSVVSAAKPKIADYHFTTIVPNLGVVETEDGRSFVMADLPGLIEGAHQGVGLGHQFLRHIERTRVIVHVIDMAAIEGRDPYEDYVVINEELKQYNLRLTERPQIIVANKMDMPNAEEHLQQFKQKLNEDVPIFPISAVTRQGIRELLFAIADLADVALLGLPNAGKSTLIRSVSEATPKVADYPFTTMYPHLGVVKVGVDSFVMADIPGVIEGAAEGAGLGLRFLKHLTRARCVLHVVDICPFNESDPVENYFAVEKELEKYSQELFDKPRFLVINKIDLLADKVEQKCQEFVEQIGYQGNYYTISAAMKKGTDELAKKLNEFLL",
    "response#2": "ADAGLVGLPNAGKSTFLAAVTRARPKIANYPFTTLHPNLGVATVDEREFILADIPGLIEGAHEGVGIGDRFLGHVERTRVLLHLVSAQEEDVAKAYTTVAHELEAYDGGLEDKPEIVALSQIDVLDEDELKKKLKALQKACSKKPMMISAITGKGMLEVLRALRDVIG",
    "response#3": "GMPNAGKSTFIRSVSRATPKVADYPFTTLVPNLGVVNPRPGQSFVIADIPGLIEGAAEGAGLGIRFLKHLERCRMLLHIIDIEPIDGVDPVYSARAIVGELEKYSPKLAAKPRWLVFNKTDLLLDEEIQAKIDRIVKELDWQGDVYTMSAYNRTGTEELSIKMLDYL"
  },
  {
    "reference": "MTLMEECRRTVPDTVRKIAEEEGVRPEKLARRVAEGRVVVPAHADRRDEVRPVGIGEGLRVKINANVGTSPEVCDPDLEVEKARAAVDHGADTVMDLSTGGDLREIRRRIMKAVDVPVGTVPVYEAAVEMTRRGRAVVDMDEDDMLRAIERHMEDGVDFMTVHCAVTLDALEDVLRRGRALGIVSRGGAIVAAWMIHHDAENPLYENFDYILELAREHDVTLSLGDAMRPGSVLDANDAAQHRELVVQGELVDRCREAGVQAMVEGPGHVPLDQIPAVVRLQKRVCDGAPFYVLGPVPTDVAPGYDHIAAAIGGAIAAYHGADFLCYVTPAEHLALPDVKDVILGVIATRIAAHAADTARGMKYARRENEEMAEARWNLDWDRQFELAIDPKKPRHYREERPPQAKELCSMCGEYCAIKILKDALEERR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.2. The protein should have a high affinity for [4Fe-4S] cluster, Zn(2+), substrate.3. The stability of the protein-[4Fe-4S] cluster complex should be optimized to maximize enzymatic activity.4. A protein with carbon-carbon lyase activity, 4 iron, 4 sulfur cluster binding is needed to enhance thiamine diphosphate biosynthetic process, thiamine biosynthetic process in a specific context.5. Ideal protein should have a binding affinity for key enzymes in the thiamine diphosphate biosynthesis pathway to enhance the overall efficiency of the pathway.6. The protein should have a low Km value for 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L-   methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-   deoxyadenosine + CO + formate + 3 H(+) + L-methionine. The designed protein sequence is ",
    "response#1": "MSNRYAPFPSSHKFYQQSLLFPNVRVPLRKILLTDGSGESPFNVYDTSGPYTDENVIIDITKGLPAIRIPWLSERADTPLPAAIASRPGDGVSVITAPDTRAALGPLAARFYGHPSRKLTVVA",
    "response#2": "MNILSSSRKIFASPDAAPDLRVPLREIILSEGAGEPNLPVYDTSGPYTDPSVTIDVNAGLARSRKQWVLERGGVC",
    "response#3": "MQPFTGSQKIYVQGSRNDIRVPMREISLDDTPTDFGGEKNPPVRVYDTSGPYTDPEVAIDLRAGLPDIRSAWIEERGDTGRLAAGSAGRAATPATGSDQAAPAGAAKPAARSGQAAPAGAAEPAARSGQAAPAGVAKQAAPAGAAKPAARSGQAAPAGAAEPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAVKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAARSGQAAPAGAAKPAAR"
  },
  {
    "reference": "MGSNNGRAMKLALVPLPKGSVLLPGVTLRIPVSNRPDLANLLSALLDQTNLGKRDGNTITFGCVPLRSPLLSNDGQQLIDDGSVDGAKKEEFDAIDAGQARKEDLFRYGTVGKVIGVQRRAYAEPFLVVQGVQRFTIKHILRERPFFEGEVVLHNERDAISSDAETVELFQQLRQLSRELITLLRLSSLLPSTGTRLSPLVARKFEVYIAKTDLSQAGNLADFMADVADPTFEEKLRVLASFALRTRLERVVELLARQVQGIKNSVKVTTISTSSFPSNSPFDISQIDPRDRELLARRVMAGLTGLTPPGAAGGRNNEDEKETNEVDELQKRLQEAELSPEARKVADKELRRLRKMNPANAEYGVCRTYLENIADIPWTKVTEDKLGPETLKRARNQLDEDHYGLETIKKRLLEYLAVLRLKQSTNQDVERQIAALTKELDAANEVLAEKDVPALSESDRVSLEAKLNLLQSRRLADKSPILLLVGPPGTGKTSLARSVATSLGRKFHRISLGGVRDEAEIRGHRRTYVAAMPGLIVNGLKKVGVANPVFLLDEIDKVGGANFQGDPSAAMLEVLDPEQNSTFVDHYINIPIDLSKVLFIATANSLDTIPAPLLDRMETITLSGYTTVEKRHIAKRHLIPKQIRANGLAEGQVVLSDEVVDKVITSYTRESGVRNLERELGSICRHKAVQYADAVDNGRLDTYNPVVALGDLEDILGIERFDEEIAEKHGRPGVVTGLVAYSTGGQGSILFIEVADMPGNGRVQLTGK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be involved in protein targeting to peroxisome, protein processing, protein import into peroxisome matrix by localizing to peroxisomal matrix and exhibiting ATP-dependent peptidase activity, ATP hydrolysis activity, ATP binding.2. For general function, the protein need meet that ATP-dependent serine protease that mediates the selective degradation of misfolded and unassembled polypeptides in the peroxisomal matrix; Necessary for type 2 peroxisome targeting signal (PTS2)-containing protein processing and facilitates peroxisome matrix protein import.3. The designed protein have Lon N-terminal, Lon proteolytic domains with specific sequence motifs that are crucial for its activity.4. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.5. The protein's active site must be carefully engineered to ensure that the amino acids involved in Hydrolysis of proteins in presence of ATP. are properly positioned for optimal catalysis.6. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.7. The protein design should include a Microbody targeting signal motif to ensure proper function. The designed protein sequence is ",
    "response#1": "LPLLLTHEGVLLPGSTMRTSVDTPGNMELVQNRLLRGTSLKSTIIGVVPNTSDPSSDREELPSLHRIGTAALAVQVVGSNWPKPHYTLLVTGLCRFQITEILKERPYPVAEVEQLDRLEQLSSKEEFKEALGDLSEQFYKYAVQLVDMLDNSVPAVAKLKRLLNNLPKELLPDVLTSIIRTTNEEKLQILDAVSLEERFKVTIPLLLRQVHERMRAYGRELAIPMKDRPPLPGRPFAINITDPEVIKSIYTIIEKREPYFVLFHVRDSNEADTDVISSKSSVYDIGVHCQIIRHTTPRPGVFNVLGYPLERCKLEELTTPTSKKTSKKAKNDDSENFPTSYLKGHKVSYASVRPIKDEPYDKASAEVRSLVESLKTLLSKLGGKNPLEKLQIKEGTDLINDPSKFADFVGSTIHGDPKKIQEILETLNIESRLSKALELLEKE",
    "response#2": "LPTYTLDSNLVLLPGIMYNVTFSRFKAAALLYRYKNFISQVSIINNLLSEYDFNSGNSNEEERSETEQKIEQSSLGQPETYHEINPSVISTEAVEGIKEFFQYETNMKTGRGGKGEKEAKSTNDGIKEFDWLTLAIIPNLDKIKDPETNYSIGDAAKLTNVVTVARIIGIVDDSTNIKLTLQAITRGVQITDDSKMNQHKNGLKTNEQVIGIDWNHNVSDLKGKFNALQKNYQQLFQSIDKFLIDYREALDYNKNNSNNKNGNLSLIKGNATINNNKNSDDKSNQDIKSQNLLTLNPLANALYMQLVGSKDFNKAFHRLEKLFSQVSKNDEYKIDNETYLRLVDLTCGILPFPNFQKLALLNKYKLDDRSVLINEMILQLKPPFAREVILQHVRTPTAVERDVQSAVMAGLKHYFERRLRVLRTGDLIAVPIDTQLGKALQESTIPGEDSAIDEVLGLIAATRPGQSLHYDDVAWFRVGHVQAMKQDTTEAVDGEEAEDLWGGIACVDISLTHLEQSGSVTGRQ",
    "response#3": "LPTYTLDSNLVLLPGIMYNVTFSRFKAAALLYRYKNFISQVSIINNLLSEYDFNSGNSNEEERSETEQKIEQSSLGQPETYHEINPSVISTEAVEGIKEFFQYETNMKTGRGGKGEKEAKSTNDGIKEFDWLTLAIIPNLDKIKDPETNYSIGDAAKLTNVVTVARIIGIVDDSTNIKLTLQAITRGVQITDDSKMNQHKNGLKTNEQVIGIDWNHNVSDLKGKFNALQKNYQQLFQSIDKFLIDYREALDYNKNNSNNKNGNLSLIKGNATINNNKNSDDKSNQDIKSQNLLTLNPLANALYMQLVGSKDFNKAFHRLEKLFSQVSKNDEYKIDNETYLRLVDLTCGILPFPNFQKLALLNKYKLDDRSVLINEMILQLKPPFAREVILQHVRTPTAVERDVQSAVMAGLKHYFERRLRVLRTGDLIAVPIDTQLGKALQESTIPGEDSAIDEVLGLIAATRPGQSLHYDDVAWFRVGHVQAMKQDTTEAVDGEEAEDLWGGIACVDISLTHLEQSGSVTGRY"
  },
  {
    "reference": "MRVMGIQRNCQHLLRWGTIILGMMMICNTTGNLWVTVYYGVPVWKDAETTLFCASDAKAHEKEVHNVWATHACVPTDPNPQEIPMENVTEKFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLNCTNATITNATVPNANDTVKQVEEIKNCSFNMTTELRDKRQKVFSLFYRLDIVKISGQNETINGSGQYRLINCNTSVITQACPKVTFEPIPIHYCAPAGFAILKCREKDFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEGNVTIRSENISNNAKIIIVQLNTSVEINCTRPGNNTRKSIHIGPGQAFYATGEVIGDIRNAHCNVSGSQWNATLEKVKEQLKKHFPNKTIKFDSPAGGDLEITTHTFNCGGEFFYCNTSGLFNSNNTDLNETITLPCRIKQIIRMWQKVGQAMYAPPIQGVIRCSSNITGILLTRDGGIENETEIFRPGGGNMKDNWRSELYKYKVVKIEPLGVAPTSARRRVVEREKRAVGLGAVFIGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNKTEKEIWNNMTWLQWDKEIDNYTDLIYHLLEESQNQQEKNEQDLLALDKWANLWNWFDISRWLWYIRIFIMIVGGLIGLRLVFAMLSVINRVRQGYSPLSFQTLTPSPEGLDRPGRIEEGSGEQDRDRSIRLVSGFLALAWDDLRSLCLFSYHRLRDCILIV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Transmembrane protein gp41: Acts as a class I viral fusion protein; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state.2. The designed protein should contain at least one YXXL motif; contains endocytosis signal motif to achieve its intended function.3. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "MRVKGIQRNCQHLWRWGTMLLGMLMICSATEQLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEIVLKNVTEYFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDLNGTSTNITDEVKIGNISDEVRNCSFNMTTELRDKKQKVYALFYKLDIVQIDKNEKEYRLINCNTSVIKQACPKISFDPIPIHYCTPAGYAILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEDEIVIRSENITDNAKTIIVHLNQSVEINCTRPSNNTITSITMGPGQVFYRTGEIIGDIRKAYCEINGTKWNEVLEQVAGKLKEHFNNKPISFQPHSGGDLEITMHHFNCRGEFFYCNTSKLFNKTYTENKTNETKNDTDTITLPCKIKQIINMWQGIGQAMYAPPISGTINCISNITGILLTRDGGNNITNETFRPGGGDIKDNWRSELYKYKVVQIEPLGIAPTRAKRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQNNLLXAIEAQQHLLQLTVWGIKQLQARVLAIERYLRDXQLLGIWGCSGKLICTTAVPWNTSWSNKSLEQIWXNMTWXQWEKEXDNYTDLIYXLLEQSQNQQEQNEQELLELDKWDSLWSWFSITNWLWYIKIFIMIVGGLIGLRIVFTVFSIXSRXRQGYSPLSLQTHLPAPRGPDR",
    "response#2": "MRVKEIRKNYQHLWKGGILLLGILMICSAEEKLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEIVLKNVTEYFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDLNGTSTNITDEVKIGNISDEVRNCSFNMTTELRDKKQKVYALFYKLDIVQIDKNEKEYRLINCNTSVIKQACPKISFDPIPIHYCTPAGYAILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEDEIVIRSENITDNAKTIIVHLNQSVEINCTRPSNNTITSITMGPGQVFYRTGEIIGDIRKAYCEINGTKWNEVLEQVAGKLKEHFNNKPISFQPHSGGDLEITMHHFNCRGEFFYCNTSKLFNKTYTENKTNETKNDTDTITLPCKIKQIINMWQGIGQAMYAPPISGTINCISNITGILLTRDGGNNITNETFRPGGGDIKDNWRSELYKYKVVQIEPLGIAPTRAKRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQNNLLXAIEAQQHLLQLTVWGIKQLQARVLAIERYLRDXQLLGIWGCSGKLICTTAVPWNTSWSNKSLEQIWXNMTWXQWEKEXDNYTDLIYXLLEQSQNQQEQNEQELLELDKWDSLWSWFSITNWLWYIKIFIMIVGGLIGLRIVFTVFSIXSRXRQGYSPLSLQTHLPAPRGPDRPEGIEEE",
    "response#3": "MRVKGIRKNYQHLWRWGTMLLGMLMICSATEQLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVKLENVTENFNMWKNDMVEQMHEDVISLWDQSLKPCVKLTPLCVTLNCTDLTNITSTTDNIKEEMKNCSFNVTSSRRDKWQKEYALLYRIDIVPIDNDNTSYRLISCNTSVITQACPKVTFEPIPIHYCAPAGFAILKCNNKTFNGKGPCTNVSTVQCTHGIKPTVSTQLLLNGSLAEEIVIRSENFTNNVKTIIVQLNGSVVINCTRPGNNTRKSISIGPGRAFYATGDIIGDIRQAHCNLSKTAWNTTLEQVVGKLREQFGNKTIIFKPSSGGDPEIVMHNFNCGGEFFYCNTTQLFNSTWHTNGTWQGIEGSNNTEDLITLPCRIKQIINMWQEVGKAMYAPPIRGNIICSSKITGLLLTRDGGEKNQSSNETFRPAGGDMRNNWRSELYKYKVVKIEPLGVAPTKARRRVVQREKRAVGIGALFLGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQNNLLXAIEAQQHLLQLTVWGIKQLQARVLAIERYLRDXQLLGIWGCSGKLICTTAVPWNTSWSNKSLEQIWXNMTWXQWEKEXDNYTDLIYXLLEQSQNQQEQNEQELLELDKWDSLWSWFSITNWLWYIKIFIMIVGGLIGLRIVFTVFSIXSRXRQGYSPLSLQTRLPAQRGPDRPEGIEEE"
  },
  {
    "reference": "MSLILTINVDNVSLEYQCFEMIGEICLAKGKFDRINNSIPELTYERHDGDGYKLEVPQITHDNVFSFIPATLLDKEHGIIDDMSAITAVGHYVVHGGIHFAESQLITPLTEGXIEQNNHLAPLHNPFSLXGIRLCRKIMPDXPQVAVFDTAFHRTIPDYAQVYPIPYEFYVEHDIRRYGFHGISHRYVSHRAAEMLGRSLSDLKLISCYLGNGCSITAIDGGQSVDTSMGFTPLEGLMMGTCSGNIDPAIIFHLARKHGMSIGDIEELLTKNSGLRGISGVELDXRELLXTTXMDNXRXXLALKMXCYXVXQYIGKYVASLGGLDVLIFTGEIGENMARIRSKICEKLGFLGIEIDPNKNGNRVCEKEINRDNATVQTLLIPTNSELMIARDTLDLVQKPPEQIDQITQFSRLVEIVEKRDTLPASTQLLDQPNQKGNTEPSHQNSKPXQPLIDGRPVEPPELKGSMNTEPPFAPSAWLSIYNEKQ",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm that is critical for acetyl-CoA biosynthetic process, phosphorylation.2. The protein must be engineered to have a high affinity for Mn(2+) in order to increase its catalytic activity.3. We require a protein that can specifically bind to the metabolites produced in the acetyl-CoA from acetate: step 1/2, Metabolic intermediate biosynthesis, acetyl-CoA biosynthesis pathway.4. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP, Mg(2+) binding.5. For general function, the protein need meet that Can also catalyze the reverse reaction. The designed protein sequence is ",
    "response#1": "ILREASPEILGIIQKHAEEAIYAPDEAMITFGQPSTFLGVIIEGQAEMRTPASWGEPRCLEVLNAGDFFGEISLLTNEPNTFNLIATRPTRALLIPAVVFEMWVTGDPKAMRILG",
    "response#2": "LFAGFDTVRLKELADGSGIEAFVPGERIAHAGDEVHFLGVVLEGEVLATIDDDSGPRELARFNPGDTFGEMALMSGENVLADLTAVSACKVMLIPLTLFRSHIMADAGAVQT",
    "response#3": "LFAGFDTVRLKELADGSGIEAFVPGERIAHAGDEVHFLGVVLEGEVLATIDDDSGPRELARFNPGDTFGEMALMSGENVLADLTAVSACKVMLIPLTLFRSHIMADAGAVQAA"
  },
  {
    "reference": "MTVVRLPDGTDRVFDNSVTVREVAESISPGLARAALAGKLNGKLVDLSEQIETDSDLVLITDKDSEGLEIIRHSCAHLLAHAVKELFPGTQVTIGPVIENGFYYDFSYERPFTPEDLVAIEKRMQEISKRALKIERKVWDRSRAINFFKDIGEHYKAQIIESIPDNEPVSLYSQGDFTDLCRGPHVPYTSKIKVFKLMKIAGAYWRGDSKNEMLQRIYGTAWVSNEEQNNYLRCLEEAEKRDHRKLGKQLDLFHTQEEAPGMVYWHPKGWVVWQQIEQYMRQTLAGNGYVEIRTPQVLDRSLWESSGHWENFRENMFITESENRHYAIKPMNCPGHVQVFNHGLRSYRDLPLRLAEFGSCHRNEASGALHGLMRVRSFTQDDAHIFCTEDQILGEVTKFIDLLNQVYINFGFSETLIKLSTRPLKRVGTEDQWDKAETALATALNQKELNWEVQPGEGAFYGPKIEFTLKDSLGRKWQCGTLQLDFSMPARLGAGYIAEDNTRKIPVMLHRAILGSMERFIGILIEHHAGALPLWLSPEQVIILNISRNQAEYAQLITDELKQSGIRASSDLRNEKISYKIREHSMQKIPYLMVVGDKEMENRTVTVRGRAGQDYGAMSVESFVVRAQEEIAKRL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr); Also edits incorrectly charged L-seryl-tRNA(Thr).2. The Zn(2+) binding site should be stable and able to withstand changes in environmental conditions.3. A protein that contributes to threonyl-tRNA aminoacylation by functioning in cytoplasm with ATP binding. The designed protein sequence is ",
    "response#1": "VRKELEDYSRAKHTAAGYEFVNTPHITKENLYQTSGHLDWYSDGMFPPMHIDAELNEDGTVRKPGQNYYLKPMNCPMHHLIYRARGRSYRELPLRLFEFGSVYRYEKSGVVHGLTRVRGMTQDDAHIYTTREQMHEELTSLLRFVLDLLSDYGLDDFYLELSTKDEAKFVGSDEVWEEATKTLEQVALDSGLQLVPDPGGAAFYGPKISVQAKDALGRSWQMSTIQLDFNMPERFNLEYTAADGTRKQPVLIHRALFGSIERFFGVLTEHYAGAFR",
    "response#2": "MQLLLIHSDYIEYETKKQTPVAEKIEESLKSCRLEEALTAFMAVESVDEANPEETIEKTVFEIEKVATQVKTDRIMLYPYAHLSSDLSSPKVAVKVFKGIETALSGKYEVKRAPFGWYKAFSISCKGHPLSELSRSA",
    "response#3": "YSIESDKRHMFLRFAACFGQFLMNHDMTISYKNLPLKMIEMTRYSFRKEQRGELVGLRRLRAFTMPDMHTLCPDMEAAISQFGEQYSMCIDILRKIGIDVSDFEVAIRFTREFYDDNREFITELAKKVDKPVLVEMWDTRFFYFVLKFEFNFVDAIAKASALSTVQIDVENAERYDINYVDANGKINRPTILHCSPSGAIERCIYALLEL"
  },
  {
    "reference": "MGYRIAVVGATGNVGREMLNILADREFPADEVVALASRKSLGQECGYGEKTLKAQDLDRFDFTGFDIALFAIGSEATKKYAPKAAKAGCVVIDNSSLYRYDSDVPLIVPEVNADAVMGYAKKNIIANPNCSTAQMVVALKPLHDRATIRRVVVSTYQSVSGAGKAAMDELWNQTKGMYVPGQEVEPKHFPKQIAFNVIPHIDVFMEDGATKEEWKMVAETKKIVDPKIRVHATCVRVPVFVGHAEAINIEFEEELDEEEAREILRKAPGILVVDKREAGGYVTPVDCVGDYATYVSRIRTDPTQDNTLSLWCVSDNLRKGAALNAVQIAELLGRRVLKKG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for phosphate, NADP(+), substrate binding.2. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.3. A protein that can modulate the overall flux of metabolites through the Amino-acid biosynthesis, L-threonine biosynthesis pathway, enhancing or suppressing its activity as needed.4. A protein with aspartate-semialdehyde dehydrogenase activity, protein dimerization activity, NADP binding is needed to enhance diaminopimelate biosynthetic process, 'de novo' L-methionine biosynthetic process, threonine biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "LAGLFKADLIEWMTSMTYQAASGGGARHMRELITQMGLIRDEVAGELADPASAILDIDKKVADKIASGTLPSNNFGVPLAGSLIPWIDVKLDNGQSKEEWKGYVETNKILGRSDNPIAIDGTCVRIGAMRCHSQAFTIKLKQKVPLDEIEQMLAEANDWVKVIPNERDISAAELTPVKITGTLDIPVGRLRKMNLGEDFLNAFSVGDQLLG",
    "response#2": "LGGLFQNGLVEWASSMTYQAASGAGAKNMRELIAGMGAIHSEVATELADPASAILEIDRKVSDFLRSDNYPKANFGVPLAGSLIPWIDVDLGNGQSKEEWKGNAETNKILGGEQPTIIEGLCVRVGAMRCHSQAITLKLKQDLPVSEIEQIIASANPWTKVVPNTKEASIHELTPAAVTGTLTTPVGRIRKLAMGGEYISAFTVGDQLLS",
    "response#3": "LAGLFKADLIEWMTSMTYQAASGGGARHMRELITQMGLIRDEVAGELADPASAILDIDKKVADKIASGTLPSNNFGVPLAGSLIPWIDVKLDNGQSKEEWKGYVETNKILGRSDNPIAIDGTCVRIGAMRCHSQAFTIKLKQKVPLDEIEQMLAEANDWVKVIPNERDISAAELTPVKITGTLDIPVGRLRKMNLGEDFLNAFSVGDQLLALS"
  },
  {
    "reference": "MVPEEVEVEIDEIPVLSEEGEEEEETYSQKVESVDKVRAKRVSLRTESLGQPLNREDEEMDKEISEKLPSKRTAKHIMEKMHLHMLCTPLPEEFLDQNVVFFLRNTKEAISEATDMKEAMEIMPETLEYGIINANVLHFLKNIICQVFLPALSFNQHRTSTTVGVTSGEVSNSSEHESDLPPMPGEAVEYHSIQLIRDEFLMNVQKFASNIQRTMQQLEGEIKLEMPIISVEGEVSDLAADPETVDILEQCVINWLNQISTAVEAQLKKTPQGKGPLAEIEFWRERNATLSALHEQTKLPIVRKVLDVIKESDSMLVANLQPVFTELFKFHTEASDNVRFLSTVERYFKNITHGSGFHVVLDTIPAMMSALRMVWIISRHYNKDERMIPLMERIAWEIAERVCRVVNLRTLFKENRASAQSKTLEARNTLRLWKKAYFDTRAKIEASGREDRWEFDRKRLFERTDYMATICQDLSDVLQILEEFYNIFGPELKAVTGDPKRIDDVLCRVDGLVTPMENLTFDPFSIKSSQFWKYVMDEFKIEVLIDIINKIFVQNLENPPLYKNHPPVAGAIYWERSLFFRIKHTILRFQEVQEILDSDRGQEVKQKYLEVGRTMKEYEDRKYEQWMEVTEQVLPALMKKSLLTKSSIATEEPSTLERGAVFAINFSPALREIINETKYLEQLGFTVPELARNVALQEDKFLRYTAGIQRMLDHYHMLIGTLNDAESVLLKDHSQELLRVFRSGYKRLNWNSLGIGDYITGCKQAIGK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should contain at least one GPAGTGKT motif motif to achieve its intended function.2. For general function, the protein need meet that Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP; Produces force towards the minus ends of microtubules; Force generating protein of respiratory cilia; Probable inner arm dynein heavy chain.3. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.4. Design the protein with a signal peptide incorporated.5. The protein should have a specific function in microtubule-based movement and should be localized to dynein complex in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "YYPAGVQRPGLGCRELIFRNLSKILPAISRDVTNWVAGTRIKSSQLLAVLLLHGEDHITQHMELLLSTLYHSCLDEENQVVINSVKSAELIGTFVNPEVFLKLILSALQKSPLASHLMTLAACIRGCSREMLKTHLTKITSVISQSDVCQGSERVLYQEQLLCCIQSLIDVCQQDCTEVSLQCMNVLITILR",
    "response#2": "YYPAGVQRPGLGCRELIFRNLSKILPAISRDVTNWVAGTRIKSSQLLAVLLLHGEDHITQHMELLLSTLYHSCLDEENQVVINSVKSAELIGTFVNPEVFLKLILSALQKSPLASHLMTLAACIRGCSREMLKTHLTKITSVISQSDVCQGSERVLYQEQLLCCIQSLIDVCQQDCTEVSLQCMNVLITILF",
    "response#3": "VQSISQTVTRWISDINQISNTKLELTNASIVDEINYWMSMERSLFFIENQLKQPEVDFTIEVLTQAKKMNITAQFKEIALKQSLQKCQSCNQFMKEFPINNLLIATNLVEIKDAMIQIFQHMKKLSNIQETYTIPRSLQLAESFSRELTNEMIKYFKGFQILHIKYVDFKGLIIKTQEIFSQWDEEYKIFKQSIVKKSVHQKDQYGQFEHIKLQKQIQHIQRLREMHENLKEVIEQIIQNDQEEQKENVQQFATLQEIQQAYDIFKNVEVFDLSRDGEDQFFRALKQYEIAIESVEATITTNLRDSLGSASSAKEMFRILAKFNKLFSRPRIKGAIQEYQSQLLKTVHKDIQSLQNKFKETYQKSQNSRLASARDIPLTSGFVIWSKQLQIRLQKYMQKVEQILGPQWAEDTDGKKCKEMGETFERILDSGPALEDWKQEINHHNKAVSQNEKLFEVVTRRRGLEIRVNYEKKLSQLFKEVRNLSNMKTKVPYSISHIANDAKASYPFALSLQESLHTYIQITSQLNAKSAKLVR"
  },
  {
    "reference": "MEVMKLTASLPQPVYSANQVLSNEAAAADAKGIPLFELMKSAGRAAFELLSQQAEQASSVLIVAGKGNNGGDGYVLARHCAAAGFMVSLLVLADEEKITGDAAKALAMLDQDSVNLRFEPDLAKANKMIAAFEGDWLVDAIFGIGLKGKLTRAWQQLLTTINEHYSQIISLDIPSGLCATTGHVENVAVAADFTVTFIVLKQGLLTGKAANYIGDLWLATLDVQTEFVQTIPAQAFAQGTSGLPAITPRRPTIHKGSIGLVLAMGGNLGMPGAIRMAAESSLRTGASLVAVACHQENQAIVVQGRPELMLAPSEPERLISSSFVEKAKVILIGPGLGRDAWANQLMQAALKADKPMVVDADALFAMKSLRAFSNNWVLTPHPGEAAFLLDCDIATIEADRFGAVTAIAQKYGGICVLKGAGSLIGDGQKVIVNTTGNAGMASGGMGDVLSGIIAALILQTGSMMDATRLAVSIHGQAAELVTQNIGMRGLLASDLFEPIRQLVNQY",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must bind to Mg(2+) in order to perform its enzymatic function.2. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.3. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.4. The protein should have a fast turnover rate for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.5. The designed protein should be able to compete with endogenous proteins or inhibitors for K(+), AMP, (6S)-NADPHX binding.6. The designed protein must be optimized for ATP binding, NADHX epimerase activity, NADPHX epimerase activity, metal ion binding, ADP-dependent NAD(P)H-hydrate dehydratase activity. The designed protein sequence is ",
    "response#1": "MRVIEENAVFLGVSGLLMMENAGRSIADLVLQFTRKIKRKAKVVVVAGLGNNGGDGLVAARHLASQGFPVTVIMADDPRLMKREEVIRNFDIIKQMRLSVKLLVYRKNMDAVKEAIENADIIIDALLGIGLQGRVREPYASLIQLINNSKGIKVSVDVPSGLDADSGNPLGPCVKADYTITFHKMKQGLVNNEYAGRIHVADIGIPLEAEVLLIGGAPGMSGALRLAGEAALRTGAGLVTLATHPQHAAWLNLTRPELMVAAVECPEALGPLVERADVIAIGPGLGRDDWGRQLWAQVRDLGLPLVVDADALSLLAEMPVYGPDWILTPHPGEAARLLQVGVREIEQDRLEAVRVLQERYGGTAVLKGAGTLINDGSQRPPAVCSDGNPGMATAGSGDVLTGMVAALRGQGMDADAAATAGVCLHAAAGDFAARQGERGLIASDIIAEPTEVGARWPVPGPASDKYTQGVTGVAAGSAEFPGAAVLASGGAVLATSGMVRFAGSAAEAVGNRWPEVIVTGSVTDAGRVQAWVVGPGIGTGSAGRDVLARVLADGVPVCADADALTLLAQHPELADERDPDVALVLTPHDREFARLTGEEPGADRATAARDAARRFGAVMLLKGNTTIVAAPDGRVLANPARSAWPATAGSGDVLAGIIGALLAAGLEPATAAAMAVHAHESAADLAANGAPAPASMLVTALTEAIRRLREG",
    "response#2": "MRAIEREAIGSGRVSGLELMERAGRGVVEAIFEEWPELRAGTFRAVVLCGPGNNGGDGFVVARLLKEWGWGVEVYLYGDAEKLPPDAQANHDRWIGMGEVLQLEVLNASVDVRPGLTVDLIVDALFGIGLTRTLPDAVTEVFCYYFRGYLDGRLVAVDVPSGLCADSGRPLVPQGTKPDLWPALMGMVAEATLTVTFHRAKPGHFLDVGPRHCGRLVVKDIGLVQAHMTRTIQRPAIAAPARDAHKYSRGLIAVLGGAMPGAARLAARGALSAGAGYVVLTGDDAGQGGPDALVRRTDPQALLNDDRLAAVLIGPGLGRDDHARALLDRALAADAPLVIDGDAISLLGQAGPERIAARTAPTWLTPHEGEFARMFAAKTGNKIDRTREAALQARATIVHKGPDTVIACPQGTAIISTHGPSWLSTAGTGDVLAGTLAARLRPGGSAAAEQAVWLHARAAHLSGPAFFADALIDHLGQAIVEAASYLRDVGKSSTEHKFTHGHALVLSGPSGRCGAARLAARGALRIGAGVVTVGCPPEAIPENAARLDAVMLREVTDAVALQSLLEDRRINALCIGPGLGTGAREAALVGVALGLSAGRPLTPPLVGEGGRSGQGAAPDDPSLPLLRVGGGQGRGTCRIVIDADALTILSQYPDLFAALHPACVLTPHAAEFARLFPDIVEKLHAPAAAGPAYSKVDATREAARRAGCTVLFKGPDTVIADPSGQCSVNAAVYDRAAPWLATAGSGDVLAGFITGLMARGFDPMRAAETAAWLHVECARQFGPGLIAEDLPEQLPGVFRSLS",
    "response#3": "MRVIEENAVFLGVSGLLMMENAGRSIADLVLQFTRKIKRKAKVVVVAGLGNNGGDGLVAARHLASQGFPVTVIMADDPRLMKREEVIRNFDIIKQMRLSVKLLVYRKNMDAVKEAIENADIIIDALLGIGLQGRVREPYASLIQLINNSKGIKVSVDVPSGLDADSGNPLGPCVKADYTITFHKMKQGLVNNEYAGRIHVADIGIPLEAEVLLIGGAPGMSGALRLAGEAALRTGAGLVTLATHPQHAAWLNLTRPELMVAAVECPEALGPLVERADVIAIGPGLGRDDWGRQLWAQVRDLGLPLVVDADALSLLAEMPVYGPDWILTPHPGEAARLLQVGVREIEQDRLEAVRVLQERYGGTAVLKGAGTLINDGSQRPPAVCSDGNPGMATAGSGDVLTGMVAALRGQGMDADAAATAGVCLHAAAGDFAARQGERGLIASDIIAGPGDVLFRIPKRPKNAIKGGNGKVLTIGGSYHYFGAPFYASSAALLSGADLSFLASPEKVAYSAAQNNPGIIPFPLDGDYLNKDHIPLLLEEAKRVDVIAIGPGLGSKEETKETVITLVSSLKGKPIVIDADALKALADVDIKLWDDVVLTPHRGEAALLAKREGDPEDLAREISKKYNATVIVKAPIDVICSPNGKCRYNKTGHPAMAVGGTGDVLTGIISGFLARRIALLKDTNALNVVASSAYVSGKAGEMAVKNKGQNITALDVLNNIQNVLKESASLV"
  },
  {
    "reference": "MTMETSRPRLILATRNSHKVGELREILAPFLPGIDLTAIHSAADLDVSEPVEDAVTFAGNALIKARHIAQATGIPAVADDSGICVDVLGGAPGIFSARWSGRHGDDRANLDLLLAQLNDVLPEYRSAYFACAATLVLPDGQEFVREGRMTGTLRYTAVGGGGFGYDPIFQPDGYDVTAAELTPDEKNKISHRGRAFAELGPIIASHVFQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for dITP + H2O = dIMP + diphosphate + H(+).2. The protein should be able to bind Mg(2+), substrate ligand in a variety of conditions3. A protein with XTP diphosphatase activity, metal ion binding, ITP diphosphatase activity, ribonucleoside triphosphate phosphatase activity is needed to enhance purine nucleoside triphosphate catabolic process, nucleotide metabolic process in a specific context.4. The presence of Mg(2+) is necessary for the protein to function in the desired environment.5. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions. The designed protein sequence is ",
    "response#1": "MKILITAGPTREPLDPVRFIGNYSSGKMGYALAEAAAARGHEVILVSGPVRLPHPPGVEKIEVKSALEMLEAVKKEMGSCDIFLSAAAVSDFTPKKVSQSKISKNEETLTLELVKNPDILSELGRSKKPGFKVGFAAETGNLVENAKRKLETKGCDFIAANLISDKGSGFGSDTNAVYLIGRNGEMEHLDLKSKEVLAKRILDKALECL",
    "response#2": "MNNELLIATNNPGKLREFRQILAPLGFKCRTLLDLQIPEETGTTFVENALIKARHGAEATGLPAIADDSGLCVRALNGEPGIFSARYAGEPKDDARNNELLLKNLQGVADRSAYFHCALVLVRHTDAADPILVEGEWHGEILRTPHGTGGFGYDPHFLVPDKHATAAELDKEQKNAISHRGKALRALLNKLAVL",
    "response#3": "MKPNEPFIVLATHNQGKLREFRAIFSDLPIDVVSMKDLDLPEPVEDGLTFAENAIIKARYVTAKTGLPAIADDSGLVVPSLDGRPGIYSARYAGPGLTDEDRNRLLLQEMSDVPDEQRTGRYYCCMVLVRHADDPEPLIAEGTWEGRILSAPRGNGGFGYDPLFIPDGYDITVAQLDPEEKNLISHRGKALRAMRPVLLDILAARKS"
  },
  {
    "reference": "MENTPRWWDISPPLSTATPTWPGDTPFQEERVWQFGPECPVNVGRVTLSPHTGAHVDAPLHYRPDGLPIGEVSLDVYMGPCRVLHCLDSGALVQPEALLGRLENLPARVLLRTYPQAPLSAWDPDFTAVAPATVELLASLGVRLIGIDTPSLDPQQSKTMDAHNTVARHGMAILEGVVLDEVAEGDYELIALPLRFAHLDASPVRAILRPLADSFLQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must be able to arylformamidase activity.2. Zn(2+) is required for the proper folding and stability of the protein.3. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects.4. The protein design should take into account the physiological conditions of the Amino-acid degradation pathway.5. The Proton donor/acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "VPVSYGINISTDIDNLKLTGQETIQVDVRTPTEDVTLNQAGLHLAGAVLDNGVKATITQDDAAETATLHFPAKVSKGAHTLVITYSGPILKTPNGIYVDDYTAPSGETKRMLVTQFEVADARRMFPGWDEPAFKATFQLNVTLPKEAVAVSNMPVTQSTPEGTSQKRVSFATTPRMSTYR",
    "response#2": "VPVSYGINISTDIDNLKLTGQETIQVDVRTPTEDVTLNQAGLHLAGAVLDNGVKATITQDDAAETATLHFPAKVSKGAHTLVITYSGPILKTPNGIYVDDYTAPSGETKRMLVTQFEVADARRMFPGWDEPAFKATFQLNVTLPKEAVAVSNMPVTQSTPEGTSQKRVSFATTPRMSTYK",
    "response#3": "VPVSYGINISTDIDNLKLTGQETIQVDVRTPTEDVTLNQAGLHLAGAVLDNGVKATITQDDAAETATLHFPAKVSKGAHTLVITYSGPILKTPNGIYVDDYTAPSGETKRMLVTQFEVADARRMFPGWDEPAFKATFQLNVTLPKEAVAVSNMPVTQSTPEGTSQKRVSFATTPRMSTYR"
  },
  {
    "reference": "MVLGGCPVSYLLLCGQAALLLGNLLLLHCVSRSHSFNATAELDLTPSGAAHLEGPAASSWEYSDPNSPVILCSYLPDEFVDCDAPVDHVGNATAYQELGYGCLKFGGQAYSDVEHTAVQCRALEGIECASPRTFLRKNKPCIKYTGHYFITTLLYSFFLGCFGVDRFCLGHTGTAVGKLLTLGGLGIWWFVDLILLITGGLMPSDGSNWCTVY",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be expressed specifically in the membrane The designed protein sequence is ",
    "response#1": "RCLNLGSYNYLGFAAATGQCADDSEESARSSGLAYCSSRCELGDNEQLQELEALTARYFGVEDAIVFGMGFATNALNLPSLLGPNSLVISDEKNHASIILGLRLSGATTKVFKHNNMRDLERVLRQGVCYGNPKKGGQPWDKVMILVEGIFSMEGSIVRLPEVIALKKKYKAYLYLDEAHSVGAMGSRGRGVTDYFNVDPKEVDILMGTFTKSFGSAGGYLAGSKKLIDFLRTNSHAHCYAASISPPIAQQILTSMKTIMGEDGTDIGRKKIHQLARNTRYFRRRLAQLGVITYGHEDSPVVPMLVYLFSKIGAVVRTLTTRHIAAVGAGFPATPIMEGRIRFCLSAAHTKEQLDFALEAIVDR",
    "response#2": "RCLNLGSYNYLGFAAATGQCADDSEESARSSGLAYCSSRCELGDNEQLQELEALTARYFGVEDAIVFGMGFATNALNLPSLLGPNSLVISDEKNHASIILGLRLSGATTKVFKHNNMRDLERVLRQGVCYGNPKKGGQPWDKVMILVEGIFSMEGSIVRLPEVIALKKKYKAYLYLDEAHSVGAMGSRGRGVTDYFNVDPKEVDILMGTFTKSFGSAGGYLAGSKKLIDFLRTNSHAHCYAASISPPIAQQILTSMKTIMGEDGTDIGRKKIHQLARNTRYFRRRLAQLGVITYGHEDSPVVPMLVYLFSKIGAVVRTLTTRHIAAVGAGFPATPIMEGRIRFCLSAAHTKEQLDFALEALA",
    "response#3": "RCLNLGSYNYLGFAAATGQCADDSEESARSSGLAYCSSRCELGDNEQLQELEALTARYFGVEDAIVFGMGFATNALNLPSLLGPNSLVISDEKNHASIILGLRLSGATTKVFKHNNMRDLERVLRQGVCYGNPKKGGQPWDKVMILVEGIFSMEGSIVRLPEVIALKKKYKAYLYLDEAHSVGAMGSRGRGVTDYFNVDPKEVDILMGTFTKSFGSAGGYLAGSKKLIDFLRTNSHAHCYAASISPPIAQQILTSMKTIMGEDGTDIGRKKIHQLARNTRYFRRRLAQLGVITYGHEDSPVVPMLVYLFSKIGAVVRTLTTRHIAAVGAGFPATPIMEGRIRFCLSAAHTKEQLDFALEAITG"
  },
  {
    "reference": "MSLQQYEILLDSGTNELEIVKFGVGENAFGINVMKVREIIQPVEVTSVPHSHQHVEGMIKLRGEILPVISLFSFFGVEPEGSKDEKYIVTEFNKRKIVFHVGSVSQIHRVSWEAIEKPTSLNQGMERHLTGIIKLEDLMIFLPDYEKIIYDIESDSGVDTYNMHTEGFDERRTDKKLIIVEDSPLLMRLLQDELKEAGYNNIASFENGKEAYEYIMNLAENETDLSKQIDMIITDIEMPKMDGHRLTKLLKENPKSSDVPVMIFSSLITDDLRHRGEVVGADEQISKPEISDLIKKVDTYVIE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Chemotaxis involves both a phosphorylation-dependent excitation and a methylation-dependent adaptation; Moreover, CheA-dependent phosphorylation of CheV is required for adaptation to attractants during B.subtilis chemotaxis..2. The protein design must be focused on enhancing the efficiency of phosphorelay signal transduction system. The designed protein sequence is ",
    "response#1": "DLITSMGQLQRNARDLQESVMSIRMMPMEYVFSRYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKRLAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEVAMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQKMGGHVEIQSKQGTGTTIRILLPLTR",
    "response#2": "MTGDLKQLVEQMSAQAERSEQAMERQRHETDQVATAINEMSAAAHEVAQSAQRAAEAAQQTDHEGQAAKRVVDGSIERIHALVDEIRDSGTSLDSLQQDVQSIVSVLGVIRSIAEQTNLLALNAAIEAARAGEAGRGFAVVADEVRALASRTQQSTQEIQGMIDRLQQGTNAAVDAMRRSGEAGEGTSNQANQAGDSLDAIAQLIATINAMNAQIASAAEEQTAVAEEINRSVHQIC",
    "response#3": "MTGDLKQLVEQMSAQAERSEQAMERQRHETDQVATAINEMSAAAHEVAQSAQRAAEAAQQTDHEGQAAKRVVDGSIERIHALVDEIRDSGTSLDSLQQDVQSIVSVLGVIRSIAEQTNLLALNAAIEAARAGEAGRGFAVVADEVRALASRTQQSTQEIQGMIDRLQQGTNAAVDAMRRSGEAGEGTSNQANQAGDSLDAIAQLIATINAMNAQIASAAEEQTAVAEEINRSVHQIL"
  },
  {
    "reference": "MLKKSIPLISALAVAVSLGMASTHAVAADETGSMANEYWWPNKLDLEPLRQHSPESNPYGDDFDYAEAFSKLDLNAVKKDIEALMTSDQEWWPADYGHYGPFFIRMAWHGAGTYRVQDGRGGAGGGQQRFEPLNSWPDNVSLDKARRLLWPVKQKYGRSISWADLMVLTGNVALESMGFKTFGFAGGRVDQWEPDIVYWGPEKKWLADERYEGDRKLDNPLAAVQMGLIYVNPEGPNGKPDPLLAAKDIRDTFGRMAMNDEETVALIAGGHTFGKAHGAHKPDECIGPDPAGAPIENQGLGWENKCGKGNAEDTITSGLEGAWSVNPIAFTTQYFDNLFGFEWEQTRSPAGAIQWIPKDGQAANLVPDAHVEGKRHAPIMFTTDLSLKFDPEYRKISKRFHENPEEFELAFAKAWFKLTHRDMGPKARYLGSEVPNEDLSWQDPIPEVDYTLINDDEIADLKEEILDSGLTVEQLVRTAWASAATFRNTDMRGGANGARLRLAPQKSWAVNDPKELAKVMKKLESIQKAFNKSLKSGKKVSLADVIVLGGAAAIEKAAKDGGHDVTVPFYPGRNDATAEMTEVDSVQYLEPKADAFRNYFGKDNRLSPAQMMVDKADTLGLTVPEMTVLIGGMRALGANTGGVKHGVFTAKPGTLTNDFFVNLLSMDTKWSKAKGTEGLYEGKDRATGKVKWTATPVDLIFGSNSELRAVAEVYAQSDSQEKFVNDFVAAWSKVMTNDRFDLE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be optimized for metal ion binding, catalase activity activity in order to improve response to oxidative stress, hydrogen peroxide catabolic process.2. The protein should be able to bind heme b ligand in a variety of conditions3. The protein must be engineered to have a high affinity for heme b in order to increase its catalytic activity.4. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for AH2 + H2O2 = A + 2 H2O.5. The protein must exhibit the following characteristics: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity..6. The presence of Plant heme peroxidase family profile domains is critical for the interaction of the designed protein with its intended targets.7. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "LDKARRLLWPVKQKYGRKLSWADLIVLAGNVALESMGFETFGFAGGREDAYEPDDAVDWGPEDEMEASDRFNEDGELENPLGATVMGLIYVNPEGPDGEPDPQASAENIRESFGRMAMNDEETAALIAGGHTFGKVHGADNPDEHVGPEPEAAPIEQQGLGWESSHGSGKGSDTITSGIEGPWNTTPTQWDMGYLDNLLGYNWWPEKGPGGAWQWTTQNGELDEAAPGVEDPSEKEDVMMLTTDIALKRDPDYREIIERFQENPKEFQKAFAKAWYKADYGHYGPFFIRMAWHAAGTYRVHDGRGGAGGGQQRFDPLNSWPDNANLDKARRLLWPIKQKYGHNLSWADLIALTGNVALESMGFKTFGYAGGREDDWEPDYVYWGPESKFLADERRDKKGKLKGPLAAVEMGLIYVNPEGPHGNPDPIASANDIRMSFGRMAMNDEEIVALIAGGHSFGKAHGAKKAGCLDVAPAAAAIEEQGFGWKNKCGKGNAEDTITSGLEGAWTVTPTRWSINYLQNLFAYNWVQTKSPAGATQWIPENNQAANLVPDAHIEGKRHAPIMFTTDIALKEDPEFNKISQRFLNP",
    "response#2": "AFARAWFKLTHRDMGPISRYLGPDVPTERFIWQDPVPVVNHKLINKKDIIHLKKKILSSGLSICELVKVAWESASTFRGSDKRGGANGARIRLEPQKNWEVNEPEELNNVLNVLEEIQVKFNKSQSGNKKVSLADIIVLAGCAGIEQAAKNAGYDIIVPFTPGRTDATQDQTDINSFSVLEPKADGFRNYIKSEISAKPEEMLIDRAQLLTLTAPEMTVLIGGMRVLNINYKQSKDGVFTNKTECLTNDFFQNLLDMNTVWKPIDEDKQRFEGCDRETGKVKWTATRVDLIFGSNSQLRAIAEVYALDKARRLLWPVKQKYGEALSWSDLIVLAGNVALENMGFQTYGFAGGRADDWEPDMVYWGPEVEMLASDREDKDGKLQRPLGATHMGLIYVNPEGPRGVPDPLGSAKNIRVAFERMAMNDEETLALIAGGHTFGKMHGAHKPADCVGAEPAAAGIEEQGFGWKNKCGKGHSEDTVTSGLEGAWTQAPTRWTSLYLSNLLNFEWKQTRSPAGAIQWIPTDESLHTAVADAHVEGKFNPPVMTTADLALKFDPEYRKIAERFLADPEEYRLAFAKAWYKLTHRDMGPPRNFLGKEVPQEV",
    "response#3": "SGLTVPELVRTAWASAASYRASDMRGGVNGARVALAPQKDWPVNNPQELAKVVGTLKSIQSDFNESARGGTRVSLADLIVLAGAAALEKAAKDAGVDIEVPFSPGRNDATQAHTDIKSFALLEPKADAFRNYYHSADNYRSPAEMLVDKADQLNLSVPEMTVLVGGMRSLNANTNGVKHGVFTKQPGSLNNHFFVNLLDMSTQWRKSANEGIYEGFDRATGALKYTATPVDLIFGSNSELRAVAEVYSR"
  },
  {
    "reference": "MNRILVASRNPKKLAELRRVLDSAGLSGVSLVSLDDVAPFDETPETGATFEDNALSKARDAFTATGVASVADDSGLEVAALNGMPGVLSARWSGSHGDDAANRALLLAQLRDVPDQRRGAAFVSACALVSVSGEVVVRGEWPGTIAREPCGDGGFGYDPVFIPAGDHRTAAQLSPVEKDAVSHRGRALALLLPALRELA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must possess ITP diphosphatase activity, metal ion binding, XTP diphosphatase activity, ribonucleoside triphosphate phosphatase activity, nucleotide binding to support nucleotide metabolic process, purine nucleoside triphosphate catabolic process under certain conditions.2. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.3. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..4. The protein should have a high affinity for substrate, Mg(2+). The designed protein sequence is ",
    "response#1": "MKKIVLATGNAGKLRDLREILGDLPIEVLPQSAFNIPECEETGTTFAENALIKARHASAATGLAAIADDSGLEVDALGGAPGIYSARFAGEGASYSDNIRKLLEDLSGVPPEKRTARFHCVLVAMRPDGAPVTAEGICEGRIAFEPRGANGFGYDPLFYLPEKGKTAAELSPGEKNAISHRGKALQKMRAALAK",
    "response#2": "MNLVFATNNQHKFEEVKAIIPADIELLTMKDIGCTEDIPETADTIEGNAILKSAYVKEKYGYDCFADDTGLEVDALNGEPGVYSARYAGENKDANANMQKVLTNLQGKENRKARFRTVIALNLNGEQHLFEGIVNGTIVRERKGEKGFGYDPVFIPDGYEQTFAEMSLEEKNSISHRGKAVNQLVEFLKTIS",
    "response#3": "MEIIFYHGTKDIQHPQIMDYFNQILDPKYTYIEWKSSERYDVEENLQKLLKELDEKVDKSKKIILAGHSLGSCIALLYAQKYKVEKIIFAAPYISSFERQTKLLKKESRKKIKTIYIYSKKDPWTPKKEVEELKNWKNSKFKVLKNGTHFNDPKYLNKIKEEIEEILK"
  },
  {
    "reference": "MISIFKILSSPKPATTTTNIINPINIINGIRYFSSNQEDYPLFKKPVFNGKVLRGGRKVFKNDLIYSKKESHYNNNNNSNNNSNNNNNNNNSNNRNNNNFSNNRNNNNNRNNNNNNSNRNNNNSNDNNFRNNNNNNRRTEYYKNDFDSSKYISNKPEIRIFDLHEQAVYGINPVWVALSSGNRTFNALYVCGTLLPKLKELGIDIKNIENSKREYISDFDHNYGRETNQIEDENQDEQYSNQHDEQEQNEYEEEQEEEKVQEEEEDNNNNNNIKKTTFNRYNDRKNLVALKEIVKNSFKLKIPVRSVNKGTLDSFSKGRPHQGIILDASPLTLLNIDFLEKFDINERVNKRMNGGGGGGGGGGGNKINNDKYPLWLVLDELWDPQNVGAIIRNCSFFNIDGVVISNKNSSPITPAASKSSSGACESFVINRTESIEGFLKSSQRNGWNVVGTSLDDLNENQPCLDINQIKLDQPTILILGNEGFGLKPSVLEICNKTIKIVGGNYKIDSLNVSVTSGILIHTLLSSGTLPK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have RNA binding and be active in mitochondrion.2. Prioritize Acidic residues, Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "VGRPVHLIGPTGCGKTALAMHVARERDRPVVWINGDADLTTSDLVGEYAEKERISERDKYVHNVVKSKDIVRDRWVDNPLTLAVREGATLVYNEFSRTKPVANNVLLSVFEEGVLELPGQRGKSRYVDVHPDFRAILTSNSVEYAGVHEPQDALLDRLVGLYLDFYDRN",
    "response#2": "PLDVILEDLESSPKSIIPILATLHRDKAFLNDISKTDLKHLVSRSLNLSKSHIAYNKWCGINLIKVLSNNYNILANEGVNFMSQLIKILENYNQSIDPKILSSTVECLNNLCNQIRGKPTLTREVLTPKLPTIITLFMEKVAYQPSLILRSLNSLIRKHPTTFRPYGNKLRNRLIELLSSESI",
    "response#3": "PLDVILEDLESSPKSIIPILATLHRDKAFLNDISKTDLKHLVSRSLNLSKSHIAYNKWCGINLIKVLSNNYNILANEGVNFMSQLIKILENYNQSIDPKILSSTVECLNNLCNQIRGKPTLTREVLTPKLPTIITLFMEKVAYQPSLILRSLNSLIRKHPTTFRPYGNKLRNRLIELLSSESS"
  },
  {
    "reference": "MAKTIKITQTRSLIGRLPKHKATMAGLGLRRIGHTVEREDTPAVRGMINLVSYMVKVEE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that can perform structural constituent of ribosome in large ribosomal subunit, cytoplasm to support translation. The designed protein sequence is ",
    "response#1": "LREYKVVGRLLPSAKNPTPPLYRMRIFAPNHVVAKSRFWYFVSQLRKMKKANGETVYCGLVHEQSPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTSGAVTQCYRDMGARHRARAHAIQIMKVL",
    "response#2": "LREYKVVGRLLPSAKNPTPPLYRMRIFAPNHVVAKSRFWYFVSQLRKMKKANGETVYCGLVHEQSPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTSGAVTQCYRDMGARHRARAHAIQIMKVR",
    "response#3": "LREYKVVGRLLPSAKNPTPPLYRMRIFAPNHVVAKSRFWYFVSQLRKMKKANGETVYCGLVHEQSPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTSGAVTQCYRDMGARHRARAHAIQIMKVR"
  },
  {
    "reference": "MDLSGKKILLGLTGGIACYKSAEFARAMIKEGATVQVVMTDGAKEFITPLTMQALTGHTVYSNQWDDQPDNKMPHIDLTRQADAVVIAPCTANFMSKLANGLGDDLLSTLCLARPAHIPFIVAPAMNAEMWKKPATQRNLNQIKEDGALVMGPASGFQACGEIGDGRMLEPAQMLEEVIAALQPKVLKNRRILITAGPTFEPIDPVRGITNRSSGKMGYAIARAAREAGASVTLISGPTALDTPYHVNRVDVMTAIEMRDAVMSHISHQDIFISVAAVADWRVANASDQKIKKNKNGDTPTLEFTENPDILASIAALPDPPYCVGFAAESEKLRQHAEEKRRKKGIPLLVGNIGHETFGKDDNTLILFDDTGTLELPHGSKQLLARQLIEQIAKRL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis pathway to avoid interference with native metabolic processes.2. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..3. The designed protein should contain one or more DNA/pantothenate metabolism flavoprotein C-terminal domains that are essential for its function.4. The designed protein must be able to perform metal ion binding under specific conditions.5. The desired protein should be able to perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2 The designed protein sequence is ",
    "response#1": "MNIVLGITGGIAAYKAPDLVRRLKERGGDVQIVMTVSAAEFVTDTALQAVSGRPVRSNLWDKEAERAMSHIELARWADVILIAPATAEVMARIVSGGAPDLLTTICLATEAPIAVAPAMNHIMWSNPATQANRKVLEERGIHILGPGVGSQACGETGAGRMLEPDTIAAAVFESLGAASATPAAAGCHILLTAGPTREEIDPVRYLTNYSSGKMGYAAAAVAARRGATVTLVSGPTNLTVPTGVKCVPVCSTGEMRAAVLEHAESVEVIIKAAAVADYRPAARAEQKMKKTGDELTLQLERTPDILAELGARKGDKILIGFAAETERLLIHAAEKLKKKNLDMIVANDVTRPGAGFNVDTNIVRLVDETGSEVWPTQSKDKVARQLAARIAETLGA",
    "response#2": "MNIVLGITGGIAAYKAPDLVRRLKERGGDVQIVMTVSAAEFVTDTALQAVSGRPVRSNLWDKEAERAMSHIELARWADVILIAPATAEVMARIVSGGAPDLLTTICLATEAPIAVAPAMNHIMWSNPATQANRKVLEERGIHILGPGVGSQACGETGAGRMLEPDTIAAAVFELLAGGPRELAAGEALAGRRVVISAGGTREPLDPVRFLGNRSSGKQGYALAEVALSRGADVTLISANVALPVPEGVTLVPVQTAIELGEAVAKAAQDCDVVVMAAAVADYRPAHYTDVKIKKTYDETVPESRPLAPTIELVRNPDVLAGLVQSRGSSTVPVIVGFAAETGDPEHSVLDLARAKFARKGCDVLVANEVGVDKTFGQDTNTVFILTPDGAAEAWPRMPKSEVARRLADRIADALG",
    "response#3": "MNIVLGITGGIAAYKAPDLVRRLKERGGDVQIVMTVSAAEFVTDTALQAVSGRPVRSNLWDKEAERAMSHIELARWADVILIAPATAEVMARIVSGGAPDLLTTICLATEAPIAVAPAMNHIMWSNPATQANRKVLEERGIHILGPGVGSQACGETGAGRMLEPDTIAAAVFAHAARVLARG"
  },
  {
    "reference": "MPKFVFVTGGVASSLGKGITAASLGRILAARGLSVASLKMDPYLNVDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFIDRNVTRASNLTTGQVYNAVIAKERRGDYLGATVQVIPHVTNEIKERIHRVAREQQAELVLVEVGGTVGDIESLPYLEAIRQFRNDVGKQNVLYIHLSLLPRVATGELKTKPTQHSVRELRAIGIQPDVLIARADEPIDEELREKIALFCDVKPDNVISEPDAWSIYAVPLMLEEAGLGRIVVDELGLSERAREPDLAEWRTLTERIRGARIPLRIGLVGKYIELTDAYLSVAESVKHASWAAGCDVKISWIDSERLERDRDVEPLIGLDGIVVPGGFGYRGIEGKVHAVRFGRESAVPTLGLCMGMQCMVIELARTALGTEDANSTEFDAFTPHPVIDLLPEQRDIADKGGTMRLGSYPCVLENGSLASRAYAQPIVLERHRHRYEFNNAYRELLAQHGMRFTGLSPDGRLVEIVELPDHPFFLGTQFHPEFKSRPNRPHPLFASFIAASVEYARANGRAQVPAPALQEAER",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates..2. The protein's Nucleophile, active site, Nucleophile; for glutamine hydrolysis must be carefully engineered to ensure that the amino acids involved in H2O + L-glutamine = L-glutamate + NH4(+) are properly positioned for optimal catalysis.3. The CTP, ATP, L-glutamine, UTP binding site should be located in a region of the protein that is accessible to the ligand.4. The protein design should take into account the physiological conditions of the CTP from UDP: step 2/2, CTP biosynthesis via de novo pathway, Pyrimidine metabolism pathway.5. A protein with ATP binding, CTP synthase activity, metal ion binding is needed to enhance 'de novo' CTP biosynthetic process, glutamine metabolic process in a specific context.6. The designed protein must have at least one Glutamine amidotransferase, CTP synthase N-terminal domain. The designed protein sequence is ",
    "response#1": "IFVTGGVVSSLGKGIAASSLAAILEARGLNVTMMKLDPYINVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFVRTRLTGRNSITTGKIYESVIRKERRGDYLGATVQVIPHITDQIKHSVYEATRGHDVALVEIGGTVGDIESLPFLEAIRQLRIEHGPEKCLFMHLTLVPFIKAAGEIKTKPTQHSVKELRSIGIQPDILVCRCEQPLPDGERRKIALFTNVPEKAVISAVDLDVIYKLPSWLHKQGLDDIVVRRLKLDVEENPDLIGWQELIDRVENPDGEITIGLVGKYVELKDAYKSISEAIIHAGAKNSCKVNIEWLHAEKINTNNVKQKLSKLKGIIVAPGFGDRGIEGKITAIKYIRENNIPFFGICLGMQSAVIEYARNVLGLRNANSTEMDSKTSXPVIDLMNEQKEVTKKGGTMRLGTYDCYLKNNSNAYNAYKNNNIKERHRHRFEFNNKYKNDLEKAGLIATGINKNKNLVEIIEIAQHPWFVGVQFHPEFKSTVDNPHPLFCGFVKAALAHKEAG",
    "response#2": "IFVTGGVVSSLGKGIAASSLAAILEARGLNVTMMKLDPYINVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFVRTRLTGRNSITTGKIYESVIRKERRGDYLGATVQVIPHITDQIKHSVYEATRGHDVALVEIGGTVGDIESLPFLEAIRQLRIEHGPEKCLFMHLTLVPFIKAAGEIKTKPTQHSVKELRSIGIQPDILVCRCEQPLPDGERRKIALFTNVPEKAVISAVDLDVIYKLPSWLHKQGLDDIVVTGMNVGDADWKDWEDLIAKVEHPQKEVSIALIGKYVELHDAYLSVAEALSHGGIANDARVDIKWVHSEDLEEDGIDLEEIFSGVDGLLIPGGFGDRGIEGKIKAIRYAREKKIPLFGICLGMQCAVIEFARSVCGLQGAHSTEFDPNTPHPVIDLMPEQKDIEKKGGTMRLGHYPCRITPGTRTMEAYGEEMIEERHRHRYEFNNEYREVLEKAGMVISGLSPDGRLVEIIELKDHPWFVGTQFHPEFKSRPNRPHPLFRDFVGAAMEHRLADSYKSLNESLVHGGIANNVKVKLEFIEAELFERENVADHLLGIHGILVPGGFGARGTEGKIKAVQFARERKVPYFGICLGMQMAIIEASRNLCGIKDATSSEFVNEGKAGEHVVGLMTEWESEDGKEQRGEDADLGGTMRLGAYPAKLIEGSKVAEIYGSTEISERHRHRYEVNINYKERLEEQGCIFSGLSPDGELPEIVERDDHPWFVGVQFHPELKSKPFDPHPLFVSFIS",
    "response#3": "KYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTIMKLDPYINVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRNKMTKRNNFTTGRVYEDVLRKERRGDYLGATIQVIPHITNAIKERIVAGGEGVDVAIVEIGGTVGDIESQPFLEAIRQLGTEIGRERAMYMHLTLVPYLGAAGEVKTKPTQHSVKELRSIGIQPDILICRSDRAIPANERAKIALFTNVPDRAVISLKDVDSIYKIPALLKSQGLDALVIERFLADSYKSLNESLVHGGIANNVKVKLEFIEAELFERENVADHLLGIHGILVPGGFGARGTEGKIKAVQFARERKVPYFGICLGMQMAIIEASRNLCGIKDATSSEFVNEGKAGEHVVGLMTEWESEDGKEQRGEDADLGGTMRLGAYPAKLIEGSKVAEIYGSTEISERHRHRYEVNINYKERLEEQGCIFSGLSPDGELPEIVERDDHPWFVGVQFHPELKSKPFDPHPLFVSFIKPNAPMPRLHDAYKSVAEALHHGGIPHDAGVDIEWVASDRLTDQAAADEVLRDFDGILIPGGFGLRGTEGMIEAIRWAREQRRPFFGICLGLQMAVVEFARHVCRLPDAHSTEFKPDAESPVIALMQAQREVVDLGGTMRLGAYAARLRSGSRAAEAYGTLEISERHRHRFEVNNAYRDVLAEFGLRLSVVSADVNLVEIIELPDHPWFLGCQFHPELKSRPTRPHPLFSAFLRAAVANKE"
  },
  {
    "reference": "FRGRDAAAKASGLVGLTDRRGCCSHPACNVDHPEICG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design should prioritize the formation of helix-helix-turn elements.2. The protein must exhibit the following characteristics: This toxin blocks alpha-3-beta-2/CHRNA3-CHRNB2 nAChR with high selectivity (IC(50)=8.67 nM (on rat) and 17.5 (on human)).3. The designed protein must be able to perform ion channel regulator activity under specific conditions. The designed protein sequence is ",
    "response#1": "GCCSTPPCAVLYC",
    "response#2": "LCCSTPPCAVLYC",
    "response#3": "MF"
  },
  {
    "reference": "MSDAKAFNLTVDEQNIAWLSIDVPGEKMNTLQAAFADEMSDILTQLKNEKGIKGLILHSLKPDNFVAGADVRMLEACKSAAEAETLAKQGQQMFQALVDLPYPVVAAIHGPCLGGGLELALACDYRVCSDADGTRLGLPEVQLGLLPGSGGTQRLPRLIGLLPALDLILTGKQLRAKKAKKLGVVDASVPQTVLLDVAKSFLEKGKKKPNKKVSTKEKLMSQTGVGRKLIFEQAAKKTREKTRGNYPAAEAILDVIRHGLEKGMTDGLELEARRFGELVMTAESRALRSIFFATTEMKKEHGSDAEPQAVSRVAVLGGGLMGAGISHVSVAKANVPVRIKDVANDGVLNALQYNYKLFEKQRKRRILSKAQLQGKMMQLSGGTDFTTFNHTDVVIEAVFEDLQLKQQMVADIEANAKPSTIFATNTSSLPIHQIAAQAKRPENVVGLHYFSPVEKMPLVEVIPHAGTSDETVSTVVALAKKQGKTPIVVKDCAGFYVNRILAPYMNEAAQVLMSGEPIDHLDTALLDFGFPVGPITLLDEVGIDIGAKIMPILINELGVRFRGPDVFDTLLNDGRKGRKSGKGFYTYKGKKKDVDKSIYKLLKLTPESKLGANDIAMRCVLPMLNEAVRCLDEGIIRSPRDGDIGAIFGIGFPPFLGGPFRYMDQIGIKRLVEIMNDHAKKYGDRFAPCDGLLTRAGLDKPFYE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. We require a protein that can specifically bind to the metabolites produced in the fatty acid beta-oxidation, Lipid metabolism pathway.2. A protein that can perform NAD+ binding, 3-hydroxybutyryl-CoA epimerase activity, enoyl-CoA hydratase activity, 3-hydroxyacyl-CoA dehydrogenase activity in cytoplasm to support fatty acid beta-oxidation.3. The designed protein must have at least one 3-hydroxyacyl-CoA dehydrogenase C-terminal, 3-hydroxyacyl-CoA dehydrogenase NAD binding domain.4. The protein have enhanced a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O.5. The protein must exhibit the following characteristics: Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA; Also exhibits 3-hydroxyacyl-CoA epimerase and 3- hydroxyacyl-CoA dehydrogenase activities.. The designed protein sequence is ",
    "response#1": "VGVLGGGLMGAGISHVSVSKAKVPVRIKDVSNDGVLGAMNYNYKLLSKQKKRRILTKAELQYRMSQLSGGTDYTAFKNTDMVIEAVFEDLALKQGMVKEIEQYGNERTIFATNTSSLPIHQIAAEAQRPENVIGLHYFSPVEKMPLVEVIPHEGTSDEVISTTVELARKQGKTPIVVADKAGFYVNRILAPYMNESAQVLLGGDCPGFFVNRVLFPYFAGFSLLMRDFGAEPDRIIARTMIPMINEVVRCLEEGVIASPAEADMALVYGLGFPPFHGGVFRYLDTMGTAAYVKLAERYLGPELEKMRVYH",
    "response#2": "VGVLGGGLMGAGISHVSVSKAKVPVRIKDVSNDGVLGAMNYNYKLLSKQKKRRILTKAELQYRMSQLSGGTDYTAFKNTDMVIEAVFEDLALKQGMVKEIEQYGNERTIFATNTSSLPIHQIAAEAQRPENVIGLHYFSPVEKMPLVEVIPHEGTSDEVISTTVELARKQGKTPIVVADCPGFFVNRVLFPYFAGFSLLLRDISEYFQKAAVLGAGIMGGGIAYQSAYKGTPIIMKDINDQALDLGLSTAAGILIKQVERGRMNAKKMAGVLNNITPSLSYDSVKDVDIVVEAVVENPKIKGMVLAEVEGVLSDDAILTSNTSTISIDLLAQSVKRPENFCGMHFFNPVNKMPLVEVIRGKDTSDETVAAVVAYAAKMGKSPIVVNDDCPGFLVNRVLFPYFGGFAKLVSALCEGSFWPRQHSKIVPLSEIIARMMIPMVNEVVRCLEEGIIASPAEADMALVYGLGFPPFHGGAFRWLDTVGSAKYLDMAQQYLPNVQRVDSKASLQSCEFLFRSSETTHGYKK",
    "response#3": "MVTIGVLGGGLMGGGIACVTAIRAGLPVRIKDVNAEGINHAMKYSWDLLSKRVQRKQMKPAERLREMARISGATDYKGFRQRDLVIEAVFEDLALKQRMVAEIEQHCMPETIFASNTSSLPIGEIAAQAQRPENIIGLHYFSPVDKMPLVEIIPHTGTCAQTIATTVSLAKKQGKTPIVVADCPGFLVNRVLFPYFGAFDLLLKEAEKTGFFVNRVLFPYFGGFSMLLRDGADFTKVDKIMERKFGWPMGPAYLLDVVGVDTAHHAQAVMAQGFPERMGKDGKDVIDALFESDRYGQKNGSGFYQYAWK"
  },
  {
    "reference": "MALQPKASLSLDGPAELGFVRFYLSMPDKPATTVRLFDRGDYYTAHAEDALMAARDVFKTQGVVKMLGAPGAKRLESVVLSKMNFEAFVRDLLLVRQYRVEVFKNKAGSRAAKDNDWHLAYKASPGNLSQFEEVLFGNSDSSTSIGVVGVKLSTVDGRRCVGVGYVDSTRRKLGLCEFADDDQFSNLEALLVQIGPKECVLPGGDAAGDMGKLRQVVRRGGILTSDRKKAEFATKDVVQDLNRLLKAKKGEAVNSAALPELENQVASASLSAVIKYLELLSDDSNFGQFELMTFDLSQYMKLDNAAVRALNLFQGSVEDTSGSQSLAALLNRCKTPQGQRLVNQWIKQPLLDKNRIEERLNLVEAFVEDGELRQTLQEDLLRRFPDLNRLAKKFQRQAANLQDCYRIYQTINQLPGVIQALEKYEGAHQMLLLAIFATPLSDLRSDFSKFQEMIETTLDLNQVENHEFLVKASFDPNLTELRETMNSLEEKMQSALASAARELGLEAGKSVKLESNAQMGFYFRVTCKEEKSLRNNKKFNTLDIQKNGVKFNNSKLSSLNEEYTKSREEYEEAQNAIVKEIVNISSGYVEPMQALNDVLAQLDAMVSFAHVATSAPVPYVRPTILDKGHGRLALRGCRHACVEVQDEVAFIPNDVVFERGRQTFHIVTGPNMGGKSTYIRQTGVVVLMAQIGCFVPCEAAEVSIVDRILARVGAGDSQLKGVSTFMAEMLETASILRSASRDSLIIIDELGRGTSTYDGFGLAWAISE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be optimized for single thymine insertion binding activity in order to improve isotype switching, negative regulation of neuron apoptotic process. The designed protein sequence is ",
    "response#1": "QTPSSVSAAVGGTVTINCQSSQSVYENGRLSWFQQKPGQPPKRLIYRASTLASGVSSRFTGSGSGTQFTLSISDVQCDDAATYYCLGNYDCSSGDSFTFGGGTEVV",
    "response#2": "THHKTPLPQGVRLGTVMRIRGVVPDQAGRFHVNLLCGEEQEADAALHFNPRLDTSEVVFNTKQQGKWGREERGTGIPFQRGQPFEVLIITTEEGFKTVIGDDEYLHFHHRMPSSNVRSVEVGGDVQLHSVKIA",
    "response#3": "QRPLLIVANRTATLVCNYTYNGTGKEFRASLHKGTDSAVEVCFISWNMTKINSNSNKEFNCRGIHDKDKVIFNLWNMSASQTDIYFCKIEAMYPPPYVYNEC"
  },
  {
    "reference": "MPPPSLLLLHSRAPLQPRPFRMSSRAAPSRVVVCSVVSAEGFISAAPILPPEGPWKQVEGGVTAAKGFKAAGIYGGLRAKGEKPDLALVACDVDATVAGAFTTNVVAAAPVLYCKRVLSTSKTGRAVLINAGQANAATLLNVSTDDILIQSTGVIGQRIKKDALLNSLPRLVGSLSSSVQGANSAAVAITTTDLVSKSIAVQTEIGGVAIRIGGMAKGSGMIHPNMATMLGVDGDTSTNDCVIAMASGLSGLSGILSLDSTEAQQFQACLDAVMQGLAKSIAWDGEGATCLIEVTVSGANNEAEAAKIARSVASSSLVKAAVFGRDPNWGRIACSVGYSGIQFDANRLDISLGAIPLMKNGQPLSFDRSAASKYLKDAGDVHGTVNIDISVADVIFYLLFLYQTICCPVSIVIINSVDCVIDREWRRKWKGMGL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design should consider the endogenous proteins and enzymes present in the Amino-acid biosynthesis, N(2)-acetyl- L-ornithine from L-glutamate: step 1/4 pathway to avoid interference with native metabolic processes.2. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile to enable effective catalysis of acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate.3. For general function, the protein need meet that Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate.4. The substrate binding site should be stable and able to withstand changes in environmental conditions.5. The designed protein localizes to the chloroplast. The designed protein sequence is ",
    "response#1": "GAELVTLFNNLGDRAVGLSGKDGALLRARRIPSDDEHSKEHVGEVTRVNSELLEMLLGQGYVPIISPVGLGEDGQTYELGSEAVAAEVASAIKAHKLIYLHDAPGILRGEELLSEFTASQLEAHLAAGAFAGGMRTRARMALKTLCGGGVERVQLPGGAEMQGTGRLADPSSVFGVLEHLLGRRQDLAADATAELPGLRLRAEAGKGGDAADAAPARLWSGDVSGAELVTLFNNLGDRAVGLSGKDGALLRARRIPSDDEHSKEHVGEVTRVNSELLEMLLGQGYVPIISPVGLGEDGQTYELGSEAVAAEVASAIKAHKLIYLHDAPGILRGEELLSEFTASQLEAHLAAGAFAGGMRTRARMALKTLCGGGVERVQLPGGEELEGTGRLADPSSVFGVLEELLAPRQEPL",
    "response#2": "GAELVTLFNNLGDRAVGLSGKDGALLRARRIPSDDEHSKEHVGEVTRVNSELLEMLLGQGYVPIISPVGLGEDGQTYELGSEAVAAEVASAIKAHKLIYLHDAPGILRGEELLSEFTASQLEAHLAAGAFAGGMRTRARMALKTLCGGGVERVVVASGNAGGAELVTLFNNLGDRAVGLSGKDGALLRARRIPSDDEHSKEHVGEVTRVNSELLEMLLGQGYVPIISPVGLGEDGQTYELGSEAVAAEVASAIKAHKLIYLHDAPGILRGEELLSEFTASQLEAHLAAGAFAGGMRTRARMALKTLCGGGVERVVIANGNAGQYTGAELVTLFNNLGDRAVGLSGKDGALLRARRIPSDDEHSKEHVGEVTRVNSELLEMLLGQGYVPIISPVGLGEDGQTYELGSEAVAAEVASAIKAHKLIYLHDAPGILRGEELLSEFTASQLEAHLAAGAFAGGMRTRARMALKTLCGGGVERVVIANGNAGQYTGAELVTLFNNLGDRAVGLSGKDGALLRARRIPSDDEHSKEHVGEVTRVNSELLEMLLGQGYVPIISPVGLGEDGQTYELGSEAVAAEVASAIKAHKLIYLHDAPGILRGEELLSEFTASQLEAHLAAGAFAGGMRTRARMALKTLCGGGVERVVIAAGNAGQYTGAELVTLFNNLGDRAVGLSGKDGALLRARRIPSDDEHSKEHVGEVTRVNSELLEMLLGQGYVPIISPVGLGEDGQTYELGSEAVAAEVASAIKAHKLIYLHDAPGILRGEELLSEFTASQLEAHLAAGAFAGGMRTRARMALKTLCGGGVERV",
    "response#3": "GAELVTLFNNLGDRAVGLSGKDGALLRARRIPSDDEHSKEHVGEVTRVNSELLEMLLGQGYVPIISPVGLGEDGQTYELGSEAVAAEVASAIKAHKLIYLHDAPGILRGEELLSEFTASQLEAHLAAGAFAGGMRTRARMALKTLCGGGVERVSAVALCAGPGAELVTLFNNLGDRAVGLSGKDGALLRARRIPSDDEHSKEHVGEVTRVNSELLEMLLGQGYVPIISPVGLGEDGQTYELGSEAVAAEVASAIKAHKLIYLHDAPGILRGEELLSEFTASQLEAHLAAGAFAGGMRTRARMALKTLCGGGVERVAAVVAGG"
  },
  {
    "reference": "MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTPETLRVRVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFAKAEFDRLWKK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design must be focused on enhancing the efficiency of transposition, DNA-mediated. The designed protein sequence is ",
    "response#1": "VDDADKLCVLAFDEMKVAAAFEYDSSADIVYEPSDYVQLAIVRGLKKSWKQPVFFDFNTRMDPDTLNNILRKLHRKGYLVVAIVSDLGTGNQKLWTELI",
    "response#2": "LQGYLDYLIIERSIAANTLSSYRRDLIRYSKHLSDRGIEDLAKVGEHDVSEFLVALRRGDPDSGVAALSAVSAARALIAVRGLHRFAVAES",
    "response#3": "DNFKTKYSKVILKNSEKIPQDLHEEVQEAFFTLRKHVCFFQDLVRIKGRGFVTPVSRILIGNPGCYDIHYSNPEISLACIALSRLNDYLQRESLLGLQEQDLAETTGPQNSPFGDGDQDYITETGAILQDQNPSLKSDSTILKERTSYNLTLLNYMDPQKMPSLKLEPYFGMGRMAVSWHKSNGKVNSGVDGGILEEKSAAGRDPDVWHVGLKVAWDIETPGLAIPLHQGDCYFMLVNSQSFFTQTFFLFVD"
  },
  {
    "reference": "MSNVRGEAAVLVLAAGAGTRMRSDTPKVLHTLAGRSMLAHALHSVANAAPGHLVVLLGHDRDRVEPAVAALAEELGRPIQVAVQQEQLGTGHAVRCALAALPDDFTGVVVVTYGDVPLLDADTLAGLIAAHSAESAAVTVLTTTLADPTGYGRVVRTADGEVIAIVEHADAGPSELTIQEINAGVYAFDATALRSALDRLEPHNAQQELYLTDVISIVRSDGLVVRAKHIDDSTLVAGVNDRVQLAELGAELNRRIVAAHQRAGVTIVDPATTWIDVDVTIGRDTVIRPGTQLLGSTRIGGHCLIGPDTTLADVTVGDQASVVRTHATSAAIGDGADVGPFTYLRPGSVLGADGKLGAFVETKNATIGTGTKVPHLTYVGDADIGEHSNIGASSVFVNYDGENKSRTTIGSHVRTGSDTMFVAPVNVGDGAYTGAGTVVREDVPPGALAVSAGPQRNIEGWVAKKRPGSKAAAAAAAASGTAEAARRAASRQAGEPTDSGDAAKP",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be optimized for glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity activity in order to improve cell wall organization.2. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc)3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.4. The UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MTAIILAAGEGTRMRSHKPKVLHEFAGKTFLQRVIGAVNAQEPDTTAVVVHFQAERVAAAAQSYDEDVVIVHQDDIPGTGRAVQCAMEQLDGEDQLSGPVLIAASDMPLLDAETLRELVAFHQESGDAATVLTTVLDDPTGYGRIVRDADGSAGGIVEHKDASEAQRAIREINSGVFAFDGALLADALKKVRTDNSQGEEYLTDVLGIVRGDGGRVGAHIVADPWLVEGVNDRVQLANLRAELNRRLLDRWMRAGVTILDPQTTWIEDDVQLDRDVTVLPGCYLQGHTTVAGEAVIGPDTTLIDATIDRGAHVERSRVQESYIGERANIGPWTYLRPGNELSADTKAGAFVEMKKAHIGEGTKVPHLSYVGDADLGTHTNIGGGTITANYDGVHKNHTTIGSDVHVGAGNLFVAPVTVGDNVTTGAGSVVRHDVPDDAMVYSENTQRNIENWVIHKRPGSAAAQAAEKARSAHDDDGDGESAVTEAAEEES",
    "response#2": "VVVLAAGQGTRMRSTTPKMLHEVGGRSLLGHVLTATTSLDARRTAVVVRHQAAVVAEHARGFLPDALIAEQGDVMGTGSAVRAALSVLDAAAHAEQAARGVVPNGHEGQVLDDGVDGAVLVVAGDLPLLDGGTLTQLVAAHTADGNAVTVLTVLTADPTGYGRVIRQADGTVSAIVEHKDATEAQRAVDEINAGIYAFDAAVLRDGLARLTTDNAQGEKYLTDLLGLARADGAPVAVHLVDDAWQTEGVNDRVQLAALGAELNRRTVRRWMLDGVTVVDPRTTWVDVDVELAEDVTILPGTQLHGATTVARGATVGPDTTLRDVEVGEDASVVRTHALLSVIGARASVGPFAYLRPGSVLGEGGKIGTFVETKNADIGAGAKVPHLSYVGDATIGEETNIGAASVFVNYDGVRKQRSTIGAHARTGADNLFVAPVHVGDGAYTAAGSVIRQDVPAGALGVSAGAGVTVVDPRATVIDADVEIGQDTRIAPFSSLHGATRIGARCAIGPHATLIDASVGDEATIIHSYLKEADIGERVSVGPFAYLRPGAVLRAGAKAGTFVEIKNSVLGPGAKVPHLSYVGDTDIGERTNIGAGTITANYDGFRKHRTTIGARVKVSVDTALIAPVSVGDDAYTAAGSVISKDVPPGALGVARARQRNVEGWVEARRAGTAAAQAAEAVADGDGEGDPD",
    "response#3": "IVLAAGQGTRMNSDLPKVLHPLGAAPLLHHAMRAGQALDPARIVVVAGHGAEAVTRAARAFDETVDVVVQAEQLGTAHAVAQAAPLLAGVPGEAVVLYGDTPFVRTETLEAMLAARSRHAVVVLGFEPADPTGYGRILRDDDGTVTAIVEHKDATEEQRAIREINAGVYAFDAAVLRDALSRLSTDNAQGEMYLTDVLSIARADGHRVGAHLAADVWETEGVNDRVQLARLGAELNRRTVERHMRAGVTILDPQTTWIEDDVQLDRDVTVLPGCYLQGHTTVAGEAVIGPDTTLIDATIDRGAHVERSRVQESYIGERANIGPWTYLRPGNELSADTKAGAFVEMKKAHIGEGTKVPHLSYVGDADLGTHTNIGGGTITANYDGVHKNHTTIGSDVHVGAGNLFVAPVTVGDNVTTGAGSVVRHDVPDDAMVYSENTQRNLEGWVERNRPGTAAAEAAERARSAAGEDTD"
  },
  {
    "reference": "MNAAEEVARRRDGDDPGHRDLFHIPPATGGDHPEVAYFAGNSLGLPPRAAAAELAADLADWARLGVEGHLEAGRAWLPYHELLTGPVSRLVGARPSEAVVMNSLTVNLHLLMASFYRPAGARTRILIEDAAFPSDSYAVRSQARWHGLDPDAAVLRLRPRPGEDVLRTADVCAVLAAEGPTVALVLLGGVNYLTGELMDIPAITAAGQAAGAVVGWDLAHAAGNVPLALHDWNVDFAAWCSYKYLNSGPGGLAGAFVHERHLGDPALPRLEGWWSTAAATRFEMAPVSRPPATVEAWQVSNPPIFAMGPVRTSLELFDKVGMTALRARSERLTGYLAELLDEVVAARPLHVITPRDPARRGCQLSVRVSGGSAGALTRRLREEYGVIADAREPDVVRLAPVPLYSTYHDCWRAADALARAVAP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should incorporate a binding pocket that specifically recognizes pyridoxal 5'-phosphate to enhance enzymatic efficiency.2. A protein that is capable of catalyzing reactions within the NAD(+) biosynthesis, quinolinate from L-kynurenine: step 2/3 pathway with high efficiency and specificity.3. A protein that contributes to quinolinate biosynthetic process by functioning in cytoplasm with pyridoxal phosphate binding.4. For general function, the protein need meet that Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively.5. The protein design should emphasize Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "PRPGEHTLREEDILKVIAEQGSTIALVIFSGVQYYTGQWFPMERITKAAKAQGCICGWDLAHAVGNVPLSLHDWGVDWAVWCSYKYLNSGPGGIAGLFVHESWDDRDDAIKEQTAKIVGALPSEVVVMETLTANLHLLMASFYKPTKDRYKIIIEGKAFPSDHVVCRPLPTRAPQPPPSALITISPPSPSDPYLSAEHILSVISQHAANTALVLLPGIQFYSGQFLDINLITRHCHALGITVGWDLAHAVGNVPLQLHDWNIDFAAWCNYKYMNGGPGVIGGLFVHEW",
    "response#2": "MSDAIKEQTAKIVGALPSEVVVMETLTANLHLLMASFYKPTKDRYKIIIEGKAFPSDHVVCRPLPTRAPQPPPSALITISPPSPSDPYLSAEHILSVISQHAANTALVLLPGIQFYSGQFLDINLITRHCHALGITVGWDLAHAVGNVPLQLHDWNIDFAAWCNYKYMNGGPGVIGGLFVHAR",
    "response#3": "PRPGEHTLREEDILKVIAEQGSTIALVIFSGVQYYTGQWFPMERITKAAKAQGCICGWDLAHAVGNVPLSLHDWGVDWAVWCSYKYLNSGPGGIAGLFVHESWDDRGAEGICVQTADDAIKEQTAKIVGALPSEVVVMETLTANLHLLMASFYKPTKDRYKIIIEGKAFPSDHVVCRPLPTRAPQPPPSALITISPPSPSDPYLSAEHILSVISQHAANTALVLLPGIQFYSGQFLDINLITRHCHALGITVGWDLAHAVGNVPLQLHDWNIDFAAWCNYKYMNGGPGVIGGLFVHDDYRAAPPSRVDAIKEQTAKIVGALPSEVVVMETLTANLHLLMASFYKPTKDRYKIIIEGKAFPSDHVVCRPLPTRAPQPPPSALITISPPSPSDPYLSAEHILSVISQHAANTALVLLPGIQFYSGQFLDINLITRHCHALGITVGWDLAHAVGNVPLQLHDWNIDFAAWCNYKYMNGGPGVIGGLFVHDPYDRAPLSTVVADAIKEQTAKIVGALPSEVVVMETLTANLHLLMASFYKPTKDRYKIIIEGKAFPSDHVVCRPLPTRAPQPPPSALITISPPSPSDPYLSAEHILSVISQHAANTALVLLPGIQFYSGQFLDINLITRHCHALGITVGWDLAHAVGNVPLQLHDWNIDFAAWCNYKYMNGGPGVIGGLFVHDPDCR"
  },
  {
    "reference": "MKFERGLEKLNIEFLKDKPLKDFTTFKIGGKARYIVFPKNIDELIEIIKLVKESGINWRIVGNCSNVLVSDKGFDGAIITTTKMDFFKTEENLIEAECGCMISQVARKACENGLKGLEFAVGIPGTVGGAVYMNAGAYDSEIKDVFECAEVLDEDLNIFKLGKSDMRFSYRHSRLKEEKLILLKATFRLQYAREEDVPPIEKANEYNQRRREKQPLQYPSAGSIFKRPPGNFAGKLIEDAGLKGYRVGNACISGKHAGFIVNLGDALAEDVRKLIYHTQKSVYEKFGVLLEPEIEFIGEFETPLFEMSTK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should consider the endogenous proteins and enzymes present in the Cell wall biogenesis, peptidoglycan biosynthesis pathway to avoid interference with native metabolic processes.2. A protein with FAD binding functions is required to enable cell division, cell cycle.3. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.4. For general function, the protein need meet that Cell wall formation. The designed protein sequence is ",
    "response#1": "DLLNLYGDGGNVRILEQRLRWRGIPVEVRRIAHGETVDFSTVDLVFMGGGPDREQKLASEQLLAMRDDLAAYVEADGPLLAICGGYQILGTQWLWGDELVEGLALIDMETRRPGTSANRLIGDMMLTSPLASRPVIGYENHAGRTYLGTGVECFGRVVGKAGFGNNDSEGVEGVRYRNVVGTYSHGPLLSKNPEVADWLSRV",
    "response#2": "IAHLLPDLLNLYGDGGNVRILEQRLRWRGIPVEVRRIAHGETVDFSTVDLVFMGGGPDREQKLASEQLLAMRDDLAAYVEADGPLLAICGGYQILGTQWLWGDELVEGLALIDMETRRPGTSANRLIGDMMLTSPLASRPVIGYENHAGRTYLGTGVECFGRVVGKAGFGNNDSEGVEGVRYRNVVGTYSHGPLLSKPEALLEYVARALR",
    "response#3": "DLLNLYGDGGNVRILEQRLRWRGIPVEVRRIAHGETVDFSTVDLVFMGGGPDREQKLASEQLLAMRDDLAAYVEADGPLLAICGGYQILGTQWLWGDELVEGLALIDMETRRPGTSANRLIGDMMLTSPLASRPVIGYENHAGRTYLGTGVECFGRVVGKAGFGNNDSEGVEGVRYRNVVGTYSHGPLLSKNPEVADWLRIHLIGVAGSGMSGIAALLLELGHNVSGSDKVSTLETDRLQRLGLRFFPQHRAEDASDADLIVVSSAIKIDNPILVSARDSSKPVVRRAEALAAVMRGL"
  },
  {
    "reference": "MKIFREVFELGNKEIILETGGMARQADGSVTVSCGNNVVLVTTVVKKSVADGTDFFPLSVHYLEKTYAAGKIPGGFLRREGRPSEEQILISRLIDRSIRPSFPDGFFNEIQIVATVLSYDGAFSPDILALIGASASLAISGAPYDDVVAGVRVGYTNGKYILNPNKQDLRDSDLDLVVSGTDDAILMVESEANSLPESVMLGGILYAHKHLKTIINSINRLAKVASKPRIEYSIYQINKFLKSQIKSQFFGEIKNAYTIASKQERNLKLNAIRKNVLEYIFSSDVDGNEYTEKEILEAFHDIEKDLVRSNILEGKPRIDGRCTETIRPINVKIGVLPGVHGSALFTRGETQALVVTTLGSDRDAQLVESLDGIEKCRYMLHYNFPPYSVGECGMVGMAPKRREIGHANLAKRATQAVFPNEEAYPYVVRVVSEILESNGSSSMATVCGSSLSMMDAGVPIAEPVAGIAMGLIKDGAKYAVLSDILGDEDHLGDMDFKVAGTRYGVTALQMDIKIKGISREILEQALEQARAGRLHILGIMNEVIKEHKEAVSDVAPQIHVMNINPAKIKDVVGRGGATVKGIVEKTGAQIDTSDSGEVKVFAKDKKSMDMAVAMIEEIVAEVEEGQVYKGKIVKLLDSGVFVNLLGSQDGYLPFSEIEQAGMKTNSLVEGQGLEVLVQNIDRGGRVKLSLVAR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'- direction.; Involved in mRNA degradation2. We are looking for a protein with broad substrate specificity for phosphate + RNA(n+1) = a ribonucleoside 5'-diphosphate +   RNA(n).3. The designed protein have KH, S1 motif domains with specific sequence motifs that are crucial for its activity.4. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.5. A protein that can perform polyribonucleotide nucleotidyltransferase activity, magnesium ion binding, RNA binding in cytoplasm to support mRNA catabolic process, RNA processing. The designed protein sequence is ",
    "response#1": "ELSEYVKNACESSLKELAYKPLAKEVRADETKAIYSETVKKAIEHFSAAFGPEALAADPSKALCLNEHIIEEEIHSFEKNVMRRMILDDGKRLDGRDTQRIAAVRVGKIEDELVLNPTLSQQDESVLDLLVVGSEQDIIMIEMRAGLKEKFVEYIDMTELEKRIFARGKQERAEMADEYYESIVQKFFEDNNIPEEEQEEYAIPLKEKYDEISKKLMRKIIIERGLRADGRLKTRVKDLAYAGIKEAVRIVAKQERHNRIGEITAQTLETLLPEYEGRESEIKGFLGDFEYELVREHIIKDGYRIDGRP",
    "response#2": "ELSEYVKNACESSLKELAYKPLAKEVRADETKAIYSETVKKAIEHFSAAFGPEALAADPSKALCLNEHIIEEEIHSFEKNVMRRMILDDGKRLDGRH",
    "response#3": "VTMETGEIARQAGGAVIVNVDDTVVMGTVVASKSAKPGQSFFPLTVDYLEKTYAAGKIPGGFFRREGRPSEGETLISRLIDRPLRPLFPEGFLNEVQVVVHVLSINPDVPSDIPALIAASAALAVSSIPFMGPIAGVNVGLVDGELIINPDVQQREVSVLDLQVAGHFDAVNMVEAGAKEVAEDKMLEAIMFGHELQKLIQFQQDFADAASKAPKVVEQDNSDHTAKVTALAEAPLREAYQIRQKGARSDKLKEISKEVMAKLSEEGDVDAVAVSDIMFEIEAKIVRSQILNGEPRIDGRDIRPIAIETSVVPRSHGSSLFCRGETQALCMATLASLNEAQELDAYGGGEQSKRFLLHYFFPPFSVGEVGRITGQSRREIGHGALAERSLLPVIPSETDFPYAIRVSSEILESNGSTSMATVCGGSLALMDAGVPMKAHVAGVAMGLILDGNRFAVLTDILGDEDHLGDMDFKVAGTANGITALQMDIKVQGITKEIMQVALAQAKEARMYILDKINETVGGIYRSKVVSVKDFGCFIEIKGKGEGLVHISELSDTPVRRVDQVVRVGEEIWVKCIGVDEKGRYKFSRKEALGAELAPRQPREPRSYNSNNGYSNNRNSYNNNNGERRSYNNNNNSYNNNSYNNNSYNNNSYNNNSYNNNSNNGYRRRNS"
  },
  {
    "reference": "MLVIVLQGLAGFLSIIAILCQKRYNKLHRSIYGLSYDLFLLDFVGNGLYLYCALHYCYSSLVREQLSQRFPLFYPLNDARSIPISSFLILKDFCVSCCCMMVLRQLYYYRSTKHIYQGISITSIIIISVFLVLGIFTYGCSISNLPLKNSGKFGVFYLEHINYLWVMANLLKCFKYVPQMSINWMGCSTVGLSSKFALISFLAESIDLLGRLVIPTNALFYEIPFNSTPFWVKLIQFVTLLVILCQVQYVYVGRKPRLPKGKL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be expressed specifically in the membrane The designed protein sequence is ",
    "response#1": "RCLNLGSYNYLGFAAATGQCADDSEESARSSGLAYCSSRCELGDNEQLQELEALTARYFGVEDAIVFGMGFATNALNLPSLLGPNSLVISDEKNHASIILGLRLSGATTKVFKHNNMRDLERVLRQGVCYGNPKKGGQPWDKVMILVEGIFSMEGSIVRLPEVIALKKKYKAYLYLDEAHSVGAMGSRGRGVTDYFNVDPKEVDILMGTFTKSFGSAGGYLAGSKKLIDFLRTNSHAHCYAASISPPIAQQILTSMKTIMGEDGTDIGRKKIHQLARNTRYFRRRLAQLGVITYGHEDSPVVPMLVYLFSKIGAVVRTLTTRHIAAVGAGFPATPIMEGRIRFCLSAAHTKEQLDFALEAIDNM",
    "response#2": "RCLNLGSYNYLGFAAATGQCADDSEESARSSGLAYCSSRCELGDNEQLQELEALTARYFGVEDAIVFGMGFATNALNLPSLLGPNSLVISDEKNHASIILGLRLSGATTKVFKHNNMRDLERVLRQGVCYGNPKKGGQPWDKVMILVEGIFSMEGSIVRLPEVIALKKKYKAYLYLDEAHSVGAMGSRGRGVTDYFNVDPKEVDILMGTFTKSFGSAGGYLAGSKKLIDFLRTNSHAHCYAASISPPIAQQILTSMKTIMGEDGTDIGRKKIHQLARNTRYFRRRLAQLGVITYGHEDSPVVPMLVYLFSKIGAVVRTLTTRHIAAVGAGFPATPIMEGRIRFCLSAAHTKEQLDFALEALDA",
    "response#3": "RCLNLGSYNYLGFAAATGQCADDSEESARSSGLAYCSSRCELGDNEQLQELEALTARYFGVEDAIVFGMGFATNALNLPSLLGPNSLVISDEKNHASIILGLRLSGATTKVFKHNNMRDLERVLRQGVCYGNPKKGGQPWDKVMILVEGIFSMEGSIVRLPEVIALKKKYKAYLYLDEAHSVGAMGSRGRGVTDYFNVDPKEVDILMGTFTKSFGSAGGYLAGSKKLIDFLRTNSHAHCYAASISPPIAQQILTSMKTIMGEDGTDIGRKKIHQLARNTRYFRRRLAQLGVITYGHEDSPVVPMLVYLFSKIGAVVRTLTTRHIAAVGAGFPATPIMEGRIRFCLSAAHTKEQLDFALEALA"
  },
  {
    "reference": "MAFETEIETKRRELNYLKHVSALTQKLKAQLDELSVQVAQMNSNAVTVKDVMANWDSVLRSISQASISLLQYTENDYETGKWASKEEHRNQQGAEDESAASESSEDNQQRQQDQLPPLPETLVRVKVADDVE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be designed to localize specifically to mitotic spindle in order to perform its role in chromosome segregation more effectively.2. For general function, the protein need meet that The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore .3. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "GVAPR",
    "response#2": "LVXAGXV",
    "response#3": "GIQ"
  },
  {
    "reference": "MKKLLSILAVFGVSAVGTTSVVACNKTESNNLSIVKTIAVPATVATANPKQVTNAEIKTALEANVLKAVQGVVKTATAADFQFDVYQDNKGTSLTTINLEEGNVEVYVQITPAKDKTVVIGETGYIKVTLPKIKVDISGVVIDQQIVEIKAADPKQVTKDELNAVNTYATLASAVLEAIKNKAPNAGASDFEITNNCDAGDYSAQKDVKVTVKAKDESPNISGEFKVNAKVKATLAPPKAG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Major membrane protein of spiroplasma.. The designed protein sequence is ",
    "response#1": "PLKEAELQVLRNQYEKEGQFVGVQTKFNYAWGLIKSDKRPEQQMGVRLLTDIFRDHTERRRECLYYLALGNYKLGNYAEARRYNDRLLENEPANLQSQSLRGLIDDKVAKEGL",
    "response#2": "PLKEAELQVLRNQYEKEGQFVGVQTKFNYAWGLIKSDKRPEQQMGVRLLTDIFRDHTERRRECLYYLALGNYKLGNYAEARRYNDRLLENEPANLQSQSLRGLIDDKVAKEGL",
    "response#3": "PLKEAELQVLRNQYEKEGQFVGVQTKFNYAWGLIKSDKRPEQQMGVRLLTDIFRDHTERRRECLYYLALGNYKLGNYAEARRYNDRLLENEPANLQSQSLRGLIDDKVAKEGAS"
  },
  {
    "reference": "MDVPDDHSACAGSLPRVPAKLEGKVATAPPRPSRTHPVAVAELAAAVGAHLTAPDRAHVQVTGATLRAQHVRAGDLFAALPGTRAHGADFADQAVAGGAVAVLTDAAGAAKVRDVPVLVHPDPRAVLGVLASRVYGDPSARLDVWGVTGTSGKTTTTYMIESALRAAGRATGLIGTVETRIGTDRLDSALTTPEAPDLQALLAVMHERGTTDVTMEVSSHALGLGRVGGVGFAVGAFTNLSQDHLDFHPDMEDYFQTKARLFDGRARLEVVCVDGEWGPRLVKDSTVTVATTRDADWTASDPTVTATGEQSFTAHGPGGARLDVELGLPGDFNVANALLAIACLHARGVPLDAVRTGLREVRVPGRMQRVDEGQDFTAVVDYSHKPAAVALALRAVRARATGRVITVLGCGGDRDTAKRPLMGQEAARRSDVLVVTDDNPRSEDPAAIREAMVRGALDVPADQRGEVVEIGDRRRAIEHAVSLARPGDVVVVAGKGHETGQEVAGVVHPFSDVEALTTAIRGVLQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..2. Incorporating a Meso-diaminopimelate recognition motif motif is critical for the successful design of the protein.3. We require a protein that can specifically bind to the metabolites produced in the Cell wall biogenesis pathway.4. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.5. The protein should have a high affinity for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate.6. The designed protein should have magnesium ion binding to facilitate cell cycle, regulation of cell shape. The designed protein sequence is ",
    "response#1": "MELKSLVSGIIIKEVTGNTNLTINGLSLDSRSTSKGDLFIALQGLQQHGLEYSQQAIARGAVALAWESSRQVPDSIPVIVAGDTRYALGLISGNFYGNPAEKLRTVGVTGTNGKTTTAALLRHLLGRRGEKTGIIGTIYNRIGDKVIPTENTTPESLDMLRLFEKMLDEGITEVVMEVSSQGIDMDRIAGLIFEVGVFTNLTQDHLDYHKTLENYMAAKGKLFSLLGNGRVVINKDDPYGLEMVRRSKGDVLTYGIDNGADFRATDLKMEAYGTEFILITPWGEEEIHTPLLGKFNVYNALAAIGAGLVLGIPVSGIKRGIENLTGVPGRFELIDAGQDFQLIVDYAHTPDGMENVLTAAKAITKNRLIVLFGCGGDRDRSKRPVMAKLAEDMADYVFLTNDNPRSEGEELILKEIKAGFSQSFSNYKVIADRKKAIAEAVNFAEKDDIVLILGRGHEDYQILKDKTVHFSDREVVREILK",
    "response#2": "MRLTDLLKGITYDLPGDTEISGVTTDSRTVSEGCLFFCKGAGYHTRYMEDALARGAVAAVISERPVTLPEGVTQILVEDTRRAMALMSSAFYGHPTQTLKVLAVTGTSGKTTTTYMLERGLSAAGCKVGLIGTTGSKIMGRDVATELTTPEAPMLQRLFAQMRDEGVTHVVMEVSSHALMLGRVTGAEFDVAAFTNLSQDHLDFHPTMEDYFEAKALFFDPGSDLRAGRSVICVDDEWGRRMAQRAEHPVTVATRGEEADVTAELLRIDDTGTQHICLNTTNTQLEFDLPMPGAFNVANAALATAVCSLRLGIPMETIREGLERAPQVPGRLERVPGPPDGPQVVIDYAHTPDALARVLRTLRPLVPGRLVVVFGAGGDRDRTKRPLMGRAVAEWADEAIVTSDNPRSEDPARIAAEIEAGMGAFPRRRILDRRAAIHAALASAGPGDLVLLAGKGHEDYQEIKGEKRHFDDKEVAREYLAQ",
    "response#3": "MKLREILAGIPLESVVGNVDVAVRGVKTNSHACGEGDLFIGIPGTRVDGGEFWPSALAAGAVAAIISPQAASKTPPTSDALVITATSLNKVCAQVCAAFYGYPSDSLKLVGVTGTNGKTTTAALLRHLLGRRGYKVGLIGTIEAIIGDRVIPAENTTPESYIVQKYFREMVEAGCDSVVMEVSSQGLMLHRVAGFTFDYGIFTNLGQDHIGPNEHKDFEDYRNSKGLLFKQCRLGIANIDDEHYADLLEGHTCELETFGISPAADLRAINVTLDPGRPGINFTVTGTNGKTTVTTLLHELYTELGFRAGLLSTVEVRIGREKHPATHTTPDALAIHGHLAEMRDAGVDYVFMEVSSHAVHQRRTLGLDFNGGVFTNLSHDHLDYHGTFAEYINAKKAFFDQLPATAFALVNADDRRGGVMVQNTAARVHRYSLRSNVDFHARLLANTPQGLQLDLGGTEVFFKLLGRFNAYNLLAAYATALAVGTDLTIENLRRGFASVQVPGRLERVPGPPDGPQVVIDYAHTPDALARVLRTLRPLVPGRLVVVFGAGGDRDRTKRPLMGRAVAEWADEAIVTSDNPRSEDPARIAAEIEAGMGAFPRRRILDRRAAIHAALASAGPGDLVLLAGKGHEDYQEIKGVKYPMDERVLIREILEEREK"
  },
  {
    "reference": "MTNIRKSHPLLKIINHSFVDLPTPSSLSSWWNFGSLLGVCLIVQILTGLFLAMHYTSDTTTAFNSVTHVCRDVNYGWLLRYLHANGASMFFICLYMHVGRGLYYGSYTYTETWNIGILLLFTVMATAFMGYVLPWTQMSFWGATVITNLLSAIPYIGTDLVQWIWGGFSVDKATLTRFFAFHFLLPFIIAALAMVHLLFLHETGSNNPTGIPSNSDMIPFHPYYTIKDILGLLIMLTVLSALVLFSPDLLGDPDNYTPANPLNTPPHIKPEWYFLFAYAILRSIPNKLGGVLALVMSILILAVIPALHTSKQQSMTFRPLSQCLFWLLVADLLTLTWIGGQPVEYPYITIGQIASILYFSILLVLMPITSVVENHLLKW",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.2. The protein must be able to metal ion binding.3. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "QDHLPRVVVVGDQSAGKTSVLEMIAQARIFPRGSGEMMTRSPVKVTLSEGPHHVALFKDSSREFDLTKEEDLAALRNEIEIRMRNSVKEGCTVSTETISLSVRGPGLQRMVLVDLPGVISTVTSGMAPDTKETIFSISKAYMQNPNAIILCIQDGSVDAERSIVTDLVSQMDPQGKRTIFVLTKVDLAEKNVASPSRIQQIIEGKLFPMKALGYFAVVTGKGNSSESIESIKEYEEEFFQNSKLLKTSMLKAHQVTTKNLSLAVSDCFWKMVRESVQDIDNDGTIDIRDKLAEMFADMDRNHDGLVDVVEMKNYCKDIGVPLDDHKAQHIVNKMDQTGSASVDLKEFQEFMK",
    "response#2": "PANPLSTPPHIKPEWYFLFAYAILRSIPNKLGGVLALVFSILILAILPLLQFSKQRSMMFRPLSQCLFWILTADLLTLTWIGGQPVEHPFIIIGQLASILYS",
    "response#3": "QDHLPRVVVVGDQSAGKTSVLEMIAQARIFPRGSGEMMTRSPVKVTLSEGPHHVALFKDSSREFDLTKEEDLAALRNEIEIRMRNSVKEGCTVSTETISLSVRGPGLQRMVLVDLPGVISTVTSGMAPDTKETIFSISKAYMQNPNAIILCIQDGSVDAERSIVTDLVSQMDPQGKRTIFVLTKVDLAEKNVASPSRIQQIIEGKLFPMKALGYFAVVTGKGNSSESIESIKEYEEEFFQNSKLLKTSMLKAHQVTTKNLSLAVSDCFWKMVRESVH"
  },
  {
    "reference": "MNPASPFATLTDRIDWQKVDGLVPAIVQDFQSSQVLMMGYMNPEALQKTLDTQQVTFFSRSKQRLWTKGETSGHVLQLKNIALDCDQDTLLVKVNPIGPTCHTGTVTCWDGDAQEESQMVWLHQLEQLLAERKNADPSSSYTASLYARGTKRIAQKVGEEGVEVALAATAGDKEELICESADLMYHLLVLLQEQGLAMNDVINKLKERHK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be designed to catalyze the following reaction 1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-   D-ribosyl)-5-[(5-phospho-beta-D-   ribosylamino)methylideneamino]imidazole-4-carboxamide.2. Ideal protein should have a binding affinity for key enzymes in the L-histidine biosynthesis, Amino-acid biosynthesis pathway to enhance the overall efficiency of the pathway.3. The designed protein should be involved in histidine biosynthetic process by localizing to cytoplasm and exhibiting ATP binding. The designed protein sequence is ",
    "response#1": "MVAHMNAEALALTIKTKTAHYYSRSRGKLWKKGETSGNLQTVTEIRTDCDQDAIWLKVVVAGHDATCHTGRRSCFG",
    "response#2": "MVAHMNAEALALTIKTKTAHYYSRSRGKLWKKGETSGNLQTVTEIRTDCDQDAIWLKVVVAGHDATCHTGRRSCFG",
    "response#3": "MVAHMNAEALALTIKTKTAHYYSRSRGKLWKKGETSGNLQTVTEIRTDCDQDAIWLKVVVAGHDATCHTGRRSCFR"
  },
  {
    "reference": "MEGRGFSGLYRNSSEELFLKTVMESPIGMPVPSMEMLGFKNVSQGFRADSEELFKRWLTNGEGYNSSSIGFSSRLSKRISTELVNGSNQLQVGVASDGRNNDKPFIQNNLLANDVSGDFNFPIRDPVDRELQPSNLFLAKAWFLSDQRMTRSRSSELRRRYSEMQNGLATQGIESICMDPQHGAEATKQEVANFNGYNYLSMCELPSQKGSFMSPSNSCSSNFNTPQFGDMDKVSSCVSMLKGTLQRRRLSSQLEKEAAEDDLNGIFYPQEPLFQTGFDQGQENWSNQTPVNVQVDSIGEVKDHGVLQTLEGSTNPVVDGFANQINQIYVGTASGEPSQSESSNAAPVISSGLDTCEGPINSNQTLCESSWKQVGVSKSSENTQNRVKGFREQIMDNLKDDKKRKSLERYGSITSAVSDDKGDTTKKRRVERSRKMAEAKERNSTPSVPSDMQAVLKRCENLEKEVRSLKLNLSFMNRKDSEQTKQIEDLQKQNEELADEKERLLEEIERILSETEKM",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Probably not involved in nodule organogenesis; Required for root infection by symbiotic rhizobia, infection thread (IT) formation, and nodule development.2. The protein should be optimized for nucleus-specific folding and stability, while maintaining protein homodimerization activity, DNA-binding transcription factor activity, sequence-specific DNA binding. The designed protein sequence is ",
    "response#1": "EWRLTARMAMPDHQAIVLLPPFLPLMRERAPNLDIVTDSLLALRRLEQGEIDLAVGQIGEAPPGYFRRRLYNDRFACLLRNGHPALEQESIIDTFSALRHAAIASDTKDGFGRVHDDLVKLDLQDPDPVLVSNVLTAGLAIVSTDLVLVVPRRVATRNAALLPLVIVDPPVELPPYEVALIWHERCHRDPDHRWLRQEIAAA",
    "response#2": "VCGALSKGQLRELNRHSLRRKIEAGCEIIAQGSESSFYSNIMRGVMKLCKVMPDGRQQIVGLQFAPDFVGRPFVRESTLSAEAATDSEICVFPRNLLDRMISETPELQRSLHDQALKEL",
    "response#3": "VCGALSKGQLRELNRHSLRRKIEAGCEIIAQGSESSFYSNIMRGVMKLCKVMPDGRQQIVGLQFAPDFVGRPFVRESTLSAEAATDSEICVFPRNLLDRMISETPELQRSLL"
  },
  {
    "reference": "MRAKEIRKNCLLLWRGGILLLGMLMICSATEKLWVTVYYGVPVWRETTTTLFCASDAKAYETEMHNVWATHACVPTDPNPQEVVLENVTENFNMWKNNMVEQMQEDIISLWDQSLKPCVKLTPLCVTLNCSNANFTNANATKNNDSTINEMNKEMKNCSFNITTGIGNKMQKEYALFYVHDVVLTNDTTTSYTLINCNTSIITQACPKVSFEPIPIHYCAPAGFAILKCNDKKFNRTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVIIRSENFTNNAKTIIAQLNQSVVINCVRPGNNTRKSIHLGPGRALYATGSIIRDIRKAYCNLNKTHWNNTLKQIVVKLSEQFGNKTIVFNRSSGGDPEIVMHSFNCGGEFFYCNTSQLFNSTWFANGTRKENGTAGNNTFITLPCRIKQIINRWQEVGKAMYAPPISGYINCSSNITGLLLTRDGGTGNKTEPEIFRPRRGDMKDNWRSELYKYKVVEIEPLGIAPTKAKRRVVQREKRAVEMLGAMFLRSLGAAGSTIGAASVALTVQARQLLSGIVQQQSNLLKAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICPTAVPWNASWSNKSLNEIWENMTWMEWDREINNYTQVIYNLLEKSQNQQEKNEQELLALDTWASLWNWFDISNWLWYIKIFLMVVGGLVSLRIVFSVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPRETEEEGGDRNRDSSERLATGFLALIWVDLRSLFLWSY",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must have at least one Retroviral envelope protein GP41-like domain.2. The protein design should include a Di-leucine internalization motif motif to ensure proper function.3. For general function, the protein need meet that Transmembrane protein gp41: Acts as a class I viral fusion protein4. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.5. The protein should be designed to localize specifically to viral envelope, plasma membrane in order to perform its role in positive regulation of establishment of T cell polarity more effectively. The designed protein sequence is ",
    "response#1": "MGAASLTLTVQARQLMSGIVQQQSNLLRAIEAQHHMLQLTVWGIKQLQARVLAIERYLKDQQLLGLWGCSGKLICTTTVPWNSSWSNKSQNDIWDNMTWMQWDREINNYTNTIYRLLEESQIQQEKNEQDLLALNSWQGLWDWLGITGWLWYIKIFFIIVGGLVGLKIIFCVLSIVKRVRQGYSPLSFQTSR",
    "response#2": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGMWGCSGKLICTTAVPWNTSWSNKSQEEIWSNMTWMQWDREVSNYTNIIYNLLEESQIQQEKNEKDLLALDSWKNLWTWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSVVNRVRQGYSPLSFQTAR",
    "response#3": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQKFLGIWGCSGKIICTTNVPWNSTWSNKTYEEIWNNMTWAEWEREINNYTSQIYELLTESQNQQDRNEKDLLELDKWASLWNWFSISNWLWYIKTFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQIPY"
  },
  {
    "reference": "MRTAFTADQIRGAEHVLMARLPEGTLMQRAAAGLAAVCADLLGRVYGRRVVLLVGSGDNGGDALYAGERLARRGAWAGAVLAGSKTHEAGLAALRQAGGRVVDGSALDAADLIIDGLVGIGVAGALREPYARLAEDANAARAPIVAVDVPSGVDASSGRVQGAAVRAKVTVTMGACKTGLLVDPGASRTGTVELVDIGLGPYLPDPDVTALTRDDVAGLLPRPGNESDKYRRGVLGVAAGSDLFTGAAVLAVGGALRAGAGMVRYAGPGAPVTQVRSHWPEAVVTELRRPSIEDVGRVQAWVLGPGLGTGDWAHELAAEVLASDVPVLIDADGLTLVAGDRGLLRRTAPVLITPHAGELARLTGADRADIEADRLAHARAAAAELGVTVLLKGSTTVVAESAGPVRVNATGTSWLSTGGTGDVLSGIAGALLAQGLSCYDAASCGAYLHGLAGRLAAEGAPLTAADVATSVPAAIRAVTGGHVR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.2. The protein must exhibit ADP-dependent NAD(P)H-hydrate dehydratase activity, NADHX epimerase activity, NADPHX epimerase activity, ATP binding.3. The protein should be designed to catalyze the following reaction (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.4. The designed protein should contain one or more YjeF N-terminal domains that are essential for its function.5. The designed protein should be able to compete with endogenous proteins or inhibitors for AMP binding. The designed protein sequence is ",
    "response#1": "MSQMDLKATTDIGIPSFLLMENAGLRIVQVIKKEFPNAKKILFFIGKGNNGGDGLVAVRHLYLSGYKVDYFLVHGEELKGDALLNLSILKNLGCYPLTEKPHKDYDLIVDAIFGTGFKPPIKGHVVEWINFINTSQIPVLSVDIPSGLSADSSQVYEPSVKADITVTFEFPKVCHILQPSAKRCGKIYVASIGLPKFLADKDSFQALLGPRPNAGHKGTFGHTLVLAGSRGKTGAAALTARGAMRSGAGLVTLGCPDEVQPVLAGKLTEAMTEGLPATPDGTISSAALPEIEKLADRKQAVAIGPGIGLHTDTQAAVLDIIRKAGVPLVADADALTAIGTDHALVSAAQSPRILTPHPGEMARLLGCSTSRVQEDRIAAARSLAETTKAVVVLKGARTIIADPAGITAINSSGNPGMASGGMGDILTGIIGGLLAQGYSAWDAARIGVYCHGRAADQMARIRGNWGYLASEVADWLPRLWDENLAQALLPLRPLDAHKGTFGTVVVAAGSVNYTGAVLLAGRAAYRAGAGLVTLAIPTPLHAALAGQFPEATWLLLPHELGVISADAAEVLIQHLGRASALLLGPGLGVEETTAEFVAALLAGTEGKAARGQIGFVPRQEKQEEKSPVLPPLVVDADGLKLLTKVADWPRRLPAGTILTPHPGEMAVLTGLTVTAIQGERLETARRFAAEWGQVVVLKGAFTVIAAPDGRTTTIPVATPALARAGTGDVLAGLIAGLRAQGVEAYDAARLAAWIHAQAGLRAAEWQGSPASVLAGDVVEMIPHVLAA",
    "response#2": "MREIDQKTVEEYGISGLVLMENAGLAVFRTVQEILGPLTDKTVLIICGRGNNGGDGFVAARHLVNHGGRVRLMLAADPEALTGDARVNYDIWRRMGQKVYSVADRNSLQVLHLALMQADLVIDALYGTGFRGEVKDRLKRLVEAVNESDRVVVSVDMPSGVDADTGAVRGAAIMADHTVTFGLPKLGLLLEPGTDYAGTVHVADISIPPGATAAVIEATAPHVFANEAADVAARWPVPGPHDDKYTQGVTGILAGSATYPGAAILCTGAAVAATSGMVRYAGSAAANVVTRWPEVVAAPRPEAAGRVQSWVVGPGLGTDESATEALRFALATELPVIVDADALTLLAGQPELVERRSAPTVLTPHAGEYQRVAGHPVGPDRVAAARELAERLGATVLLKGNVTVITDGETTYLNPAGQSWAATAGSGDVLSGIIGALLASGLPPTEAAGMAAYVHARAAGLSAADPGPRAVPTSAEGILAHLRSSVAQE",
    "response#3": "MKQLDQATIQTMGIPSLVLMERAALAVFGCLKQHFSLKKTLVVCGSGNNGADGMAVARLLHLAGYSVNLYLAGNPASFTEEMKIQWQAAENYQVSIVNNCRFSEYTTIVDALFGVGLSRELSGKYQTLVDAINASGVPVLAVDIPSGIHAGTGQVMGTAVRAAETVTFAYRKLGISLYPGTVYAGNVQVKDVGIYGASEVLLIAGSRNMSGAAYFSSKAALVSGAGMVRILTDESNRTILQQQFPEAMLSVYDSSMQHEKLADVLKKAMQWADVIAIGPGLSTSKTAEDLLQMVLTFRQSKPIVMDADALNLLAKRKDLQSQCDASCILTPHMGEMSRLADCTIGDLKAHPQEAMETLYRQCPCTLIMKDARTWIRTADGVYYLNLTGNHGMATAGSGDVLCGITAGLLAQGMRSSQAAPFAAWLHGLAGDHAAQEKGARSMTASDILN"
  },
  {
    "reference": "MTLITRADCQTRDRADPLAPFRDRFVLPQNLVYLDGNSLGALPKAAAARIKRSVEAEWGQDLIKSWNLHRWIDLPQRAGAKIARLVGAKAGEVIVADSTSVNLFKLLAAALAMRPGRRVILSEPGNFPTDLYVAQGLIEMLGGKHELKLVPAEKIIGAIDDKVAVVSLTHVNYKSGRIHDMAAVTAKAHAAGALMLWDLCHSAGALPVDLNAAKADLAVGCGYKYLNGGPGAPAFLFVAGRHQEAIRPPLSGWMGHAHPFTFDVDYEPAEGIARNLCGSPPVLSMSALDAALDVMLEADMAAIRMKSLALGDLFLALIEQECGTHGFVNGSAPRSERGSQVGLLHPEGYAIMQALIAKGVIGDFRAPDMLRFGFAPLYNRYLDVWEAVAGLKTVMDARTWDAPKFRTRAAVT",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be optimized for activity in quinolinate from L-kynurenine: step 2/3, NAD(+) biosynthesis, Cofactor biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. A protein that contributes to L-kynurenine catabolic process, tryptophan catabolic process, anthranilate metabolic process by functioning in cytoplasm with kynureninase activity.3. pyridoxal 5'-phosphate is required for the proper folding and stability of the protein.4. We are looking for a protein with broad substrate specificity for H2O + L-kynurenine = anthranilate + H(+) + L-alanine.5. The pyridoxal 5'-phosphate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "ADDRAAEMIAPIVGAQTSEVAVMQTLTANLHLLLSAFYQPKADSRHKIILEKKAFPSDHFAVETQIRHHGLAVESSMVMIETLRSESTALTTQDVVAVIEEHAKDTAVLLLPGIQYYTGELLDIPTITELAHRHGIFVIWDLAHAVGNVPLRLHDWNVDAAVWCSYKYLNSGPGGIGGLFVHTQNSGVIRPG",
    "response#2": "ADDRAAEMIAPIVGAQTSEVAVMQTLTANLHLLLSAFYQPKADSRHKIILEKKAFPSDHFAVETQIRHHGLAVESSMVMIETLRSESTALTTQDVVAVIEEHAKDTAVLLLPGIQYYTGELLDIPTITELAHRHGIFVIWDLAHAVGNVPLRLHDWNVDAAVWCSYKYLNSGPGGIGGLFVHTQNSGVIRPGTELPPLLADDRAAEMIAPIVGAQTSEVAVMQTLTANLHLLLSAFYQPKADSRHKIILEKKAFPSDHFAVETQIRHHGLAVESSMVMIETLRSESTALTTQDVVAVIEEHAKDTAVLLLPGIQYYTGELLDIPTITELAHRHGIFVIWDLAHAVGNVPLRLHDWNVDAAVWCSYKYLNSGPGGIGGLFVHTQNSGVIRPGAELPPLLPPFELPPLKCGFSFATNEQNEFIQLVKDNYGPDVVEIMPTKELVNAQRQDPDTWAAMRRDYLIARGL",
    "response#3": "ADDRAAEMIAPIVGAQTSEVAVMQTLTANLHLLLSAFYQPKADSRHKIILEKKAFPSDHFAVETQIRHHGLAVESSMVMIETLRSESTALTTQDVVAVIEEHAKDTAVLLLPGIQYYTGELLDIPTITELAHRHGIFVIWDLAHAVGNVPLRLHDWNVDAAVWCSYKYLNSGPGGIGGLFVHTQNSGVIRPGV"
  },
  {
    "reference": "MAQTKYIFVTGGVTSSLGKGIIAASLAKLLQARGYKTTIQKLDPYINVDPGTLNPYEHGECYVTNDGAETDLDLGHYERFLNVSTSQANNVTTGRIYQSVIEKERRGEFLGKTVQVIPHITNEIKERVQVLGKSGDYDIVITEIGGTVGDIESLPYIEAVRQLLWELGEDNAIVIHLTLIPFLSAAGELKTKPTQHSVKTLMESGIQADILVCRTEHELSTDLRDKLALFCNVKREAVIQSIDASTIYDVPNLMLKEGLDKVTLKKLRLPEDNQPDLTRWNQFLQRHKNPKNEVNIGLIGKYVELQDSYKSILEAFIHAGAANEVKVNITSVHSEYIDNRTIKEKIAHLDGILVAPGFGERGIEGKIATVQYAREHNIPFLGICLGMQMAVIEFARNVLGLENANSTEMNAKCDHPVIDLMEEQKDITDKGGTMRLGAWKCELKPGSVVSEIYSEKIIEERHRHRYEFNNKYKEQIEAAGLVTTGFNPDTQLVEIVELPSHRWFVGVQYHPEYKSTVANPHPLFVAFVKAALSYKKEKN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein's Nucleophile, Nucleophile; for glutamine hydrolysis must be carefully engineered to ensure that the amino acids involved in H2O + L-glutamine = L-glutamate + NH4(+) are properly positioned for optimal catalysis.2. The designed protein should be optimized for CTP synthase activity, metal ion binding, ATP binding activity in order to improve 'de novo' CTP biosynthetic process, glutamine metabolic process.3. The designed protein should be able to compete with endogenous proteins or inhibitors for L-glutamine, ATP, UTP binding.4. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen5. The protein contains novel Glutamine amidotransferase domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MFITGGVVSSLGKGIAAASLAAVLEARGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFVCMTATRRNNFTTGRIYENVIRKERRGDYLGGTVQVIPHITDEIKRCIRLGAGEADIAMIEIGGTVGDIESLPFLEAIRQMGVELGRERSLFIHLTLVPFIKSSGELKTKPTQHSVKELRSIGIQPDILMCRSDREIPAEERRKIALFTNVEPKAVINVPDADTIYRIPLLLRAQGVDDIVARKLQEQLQLDDNQPDLKQWQSFVDRIKNPQGEVSIAVVGKYVSLPDAYLSIIEALGHAGVANGVHVNVCLVDGEELNADNAEEMLGKMDGILVPGGFGQRAFEGKIEAAHYARVNNVPFLGICLGLQAAVCEYARNVAHLTGATSAEFDENADYTVIDLMPEQEDIEDKGGTMRLGAYPCKLDKDSLAYQVYGEEIIYERHRHRYEVNNAYRDTLTSAGLRVSGLSPDGRLTEMIEIPDHPWFLASQGHPEFKSRPTNPHPLFIGFVKAASEYQKQHA",
    "response#2": "MFITGGVVSSLGKGIAAASLAAVLEARGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFVCMTATRRNNFTTGRIYENVIRKERRGDYLGGTVQVIPHITDEIKRCIRLGAGEADIAMIEIGGTVGDIESLPFLEAIRQMGVELGRERSLFIHLTLVPFIKSSGELKTKPTQHSVKELRSIGIQPDILMCRSDREIPAEERRKIALFTNVEPKAVINVPDADTIYRIPLLLRAQGVDDIVARKLQEKLAQPDCSEWQSLVQRLQSPKHTLTDAYKSLNEALLHAGIHAHTRVNIHYIDSERVERDGTACLEHMDAILVPGGFGERGIEGKIAAVRFAREHRLPYLGICLGMQVAVIEFARHVAGLEGAHSTEFRKDTPHPVIALITEWIDEDGSVQQRREGGDLGGSMRLGAQPCRLVADSLAGATYGKDVIVERHRHRYEFNNRYRGPLGAVGMRFCGHSLDGRLVEMIELPDHPWFLACQFHPEFTSTPRAGHPLFRGFLDFLQGL",
    "response#3": "MFITGGVVSSLGKGIAAASLAAVLEARGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFVCMTATRRNNFTTGRIYENVIRKERRGDYLGGTVQVIPHITDEIKRCIRLGAGEADIAMIEIGGTVGDIESLPFLEAIRQMGVELGRERSLFIHLTLVPFIKSSGELKTKPTQHSVKELRSIGIQPDILMCRSDREIPAEERRKIALFTNVEPKAVINVPDADTIYRIPLLLRAQGVDDIVARKLQLTDAYKSLNEALLHAGIHAHTRVNIHYIDSERVERDGTACLEHMDAILVPGGFGERGIEGKIAAVRFAREHRLPYLGICLGMQVAVIEFARHVAGLEGAHSTEFRKDTPHPVIALITEWIDEDGSVQQRREGGDLGGSMRLGAQPCRLVADSLAGATYGKDVIVERHRHRYEFNNRYRGPLGAVGMRFCGHSLDGRLVEMIELPDHPWFLACQFHPEFTSTPRAGHPLFRGFLFAAAGLLTDAYKSLNEALLHAGIHAHTRVNIHYIDSERVERDGTACLEHMDAILVPGGFGERGIEGKIAAVRFAREHRLPYLGICLGMQVAVIEFARHVAGLEGAHSTEFRKDTPHPVIALITEWIDEDGSVQQRREGGDLGGSMRLGAQPCRLVADSLAGATYGKDVIVERHRHRYEFNNRYRGPLGAVGMRFCGHSLDGRLVEMIELPDHPWFLACQFHPEFTSTPRAGHPLFRGFLQAAAQFKP"
  },
  {
    "reference": "MKLRDLLAQVSNISHQPAEAVLDREVTGLSTNSHACKPGDLFIGMPGTRVDGGTFWPSAIASGAVAAVITQEAAQKQPPETATDPVISVTDMTQTCADLAAAFYGYPAKQLSMVGVTGTNGKTTTTHLIEYLLNQYPVALLGTLYTRWPGFSQVAVHTTPFSVELQEQLSAAVKAGAKFAVMEVSSHSLDQGRVLGCGFEVAVFTNLTQDHLDYHKTMEDYFEAKALLFSDRYLQGRAVINIDDDYGKRLCVRDSVAEQYRLPKEKYWTYSIEDSSADLWTADVTYSPSGVTGILHTPVGNADFSLPLVGKYNVSNMLAAVGAALHLGLELSEIVAALPNFTGVPGRMEKVQITPDQDLTVIVDYAHTPDSLENVLKATRPFVSGDLICVFGCGGDRDRTKRPLMGGIAARLSDKAVVTSDNPRTEDPEKILEDILAGIDPETSPLVICDRAEAIRRAIMEAKPGDGIVIAGKGHEDYQILGTEKIHFDDREQARAALEEKLALVSE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein have Mur ligase N-terminal catalytic, Mur ligase central domains with specific sequence motifs that are crucial for its activity.2. The designed protein should have a binding site that is specific to the target UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate ligand.3. We require a protein that can specifically bind to the metabolites produced in the Cell wall biogenesis, peptidoglycan biosynthesis pathway.4. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.5. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in cell wall organization, cell cycle. The designed protein sequence is ",
    "response#1": "MKLKDLLYKVSIKSVKGSTSIVTGITADSRAVRTGYLFAALSGSKADGRAFIAQAVTMGASVILGAEGVSDIKASYIRVEDSRKAYALISSNFYDEPAEKLKLIAVTGTNGKTTIAQLIAQWLTLSDKKAAVMGTAGNGFPGQLKPAINTTGSAVAIQKHLSELLDQEADYTAMEVSSHGLVQGRVKALNFDVGIFSNLSRDHLDYHGDMDAYGEAKKLLFTTHQCRHAVINADDPLGKRWLIEMENAVAVSLVGRPEAKSALWATEIQYTESGMTLSFDSSWGTGTFSVPLIGEFNASNILLSLAIYGTADLLGLEKEDILTALSSIKGVSGRMELFHTPGKAKLVVDYAHTPDALEKALSALRGHCQGNLWAIFGCGGNRDKGKRPMMAKIAESLADKIVLTDDNPRNESPELIIQEMVSGVNHPENISVEHSRYEAIQYALEHSQAKDIILIAGKGHEDYQIIQGNKIHFNDREVVKDLLN",
    "response#2": "MRLSDILKHLEYSIISGDKNVAVTGITADSRAVRTGYLFAALSGSKADGRAFIAQAVTMGASVILGAEGIDVDVPGIVIPDTRRAAALISSRFYGHPSSALKLAGITGTNGKTSVTFLLESILAAAGRKTGITGTNGKTTTATLLYELFKKSGYKTGLLSTVCNYIHNEEIPATHTTPDPVQLNALLARMVETGCQYCFMEVSSHSIHQHRIAGLKFAGGIFSNITHDHLDYHKTFDEYIKAKKMFFDNLPGEAFALSNFDDRNGKVMLQNTNAKKYSYSLRSMADFKCRILENHFEGMKLNMDGNEIWTRFIGKFNAYNLLAVYSITSLTMDSRRVGPGSLFVAVRGTLTDGHAFIETAVRQGAAAILCEDLPDNTADGVAYVRVQNTPHTLGLIAAAFGINADNAAGIDVSGVTADSRAVTPGSLFCCVRGTKSDGHDFAPAAVAAGATAVICERPLDLAPHVVQAVVADGRAALAAAAAAFHRHPSRSLRECAEIFVYGENIRGITADSRQVRPGSLFLAYPGEHVDGRRYIEAALRQGASAVLWEEADADTGLGKGLPGFAVRDLRAQAGQLADAFGCSPDYSIMRGVTEVKGRMQRVDAGQPYLAVVDYAHKPAAVGALLDALRAQVREAGGGLLVVLGCGGDRDRGKRPLMGAIAAARAELLIISDDNPRTEDPAEIRRAMREGAVAEPGRGEVREIGDRRAAIAAVVARARPGDAVVVAGKGHED",
    "response#3": "MTIADLAQRLGAEVRGDGSVDVTGITADSRAVRTGYLFAALSGSKADGRAFIAQAVTMGASVILGAEGIELPDGLPAPVVRAADPRRTFAAVAAAFFGDQPATVVAVTGTNGKTTTTSLLEYLWSGSGLPAGVIGTLGARYHTQGKTVSLPSARTTPEASELQKNLALMRDSGVQVVAMEVSSHALSLHRVDGIYFELSLFTNLTQDHLDFHHTMEEYFLAKSALFTSKFSAGALVNADDSFGKRLLAQCEIPAISISTDKGDWQLSLTGFQLTRPGGAIHIIDSPLVGDFNRMNAAMALVGGLRALGHPLADVVAGCAAVTPVPGRFERIDAGQPFALVVDYAHTPDALEKVLTAARGMTGTTRSPGRVIVVFGCGGGRDRAKRPLVGEVAARLADLAILTSDNPRHEDPLAIIDEVLTGVGTAFEASLVVQPDRGAAIEEAVARAEPGDVVVVAGKGHEDYQILGTEKRPFDDREEARAALDARIGGKG"
  },
  {
    "reference": "MVKSSLQRILNSHCFAREKEGDKPSATIHASRTMPLLSLHSRGGSSSESSRVSLHCCSNPGPGPRWCSDAPHPPLKIPGGRGNSQRDHNLSANLFYSDDRLNVTEELTSNDKTRILNVQSRLTDAKRINWRTVLSGGSLYIEIPGGALPEGSKDSFAVLLEFAEEQLRADHVFICFHKNREDRAALLRTFSFLGFEIVRPGHPLVPKRPDACFMAYTFERESSGEEEE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have ornithine decarboxylase inhibitor activity within cytosol, nucleus to regulate negative regulation of polyamine transmembrane transport, positive regulation of intracellular protein transport. The designed protein sequence is ",
    "response#1": "AACTLVCKIHAKREIRNEAGKLDTVMYYLNDGVYGSFNCILYDHQVVIAEHYLDNAESLPHLKSLIWGPSCDALDKISEDLHLPNLNRGDLLGFRNMY",
    "response#2": "VLISAASVNLGSMMCYSILGPFFPKEAEKKGASNTIIGMIFGCFALFELLASLVFGNYLVHIGAKFMFVAGMFVSGGVTILFGVLDRVPDGPVFIAMCFLVRVMDAVSFAAAMTASSSILAKAFPNNVATVLGSLETFSGLGLILGPPVGGFLYQSFGYEVPFIVLGCVVLLMVPLNMYILPNYESDPGEHSFWKLIALPKVGLIAFVINSLSSCFGFLDPTLSLFVLEKFNLPAGYVGLVFLGMALSYAISSPLFGLLSDKRPPLRKWLLVFGNLITAGCYMLLGPVPILHIKSQLWLLVLILVVSGLSAGMSIIPTFPEILSCAHENGFEEGLSTLGLVSGLFSAMWSIGAFMGPTLGGFLYEKIGFEWAAAIQGLWALISGLAMGLFYLLEYSRRKR",
    "response#3": "VLISAASVNLGSMMCYSILGPFFPKEAEKKGASNTIIGMIFGCFALFELLASLVFGNYLVHIGAKFMFVAGMFVSGGVTILFGVLDRVPDGPVFIAMCFLVRVMDAVSFAAAMTASSSILAKAFPNNVATVLGSLETFSGLGLILGPPVGGFLYQSFGYEVPFIVLGCVVLLMVPLNMYILPNYESDPGEHSFWKLIALPKVGLIAFVINSLSSCFGFLDPTLSLFVLEKFNLPAGYVGLVFLGMALSYAISSPLFGLLSDKRPPLRKWLLVFGNLITAGCYMLLGPVPILHIKSQLWLLVLILVVSGLSAGMSIIPTFPEILSCAHENGFEEGLSTLGLVSGLFSAMWSIGAFMGPTLGGFLYEKIGFEWAAAIQGLWALISGLAMGLFYLLEYSRRKT"
  },
  {
    "reference": "MSILSGKRIVLGVTGSIAAYKAADLASKLTQSGAQVDVILTGSAEKFVAPLTFQSVTGRRAYTDQDLWGNEAHVLHVGLGHDTDLLVIAPCTANTLAKLAQGLAGTLLTVTALASRAPLIVAPAMDGGMFDHPAMQANLETLRGRGVTIIEPAEGHLASGLTGTGRLPETHELIGHIRLVLGRRGLLADKRVLVTAGGTQEPLDPVRVLTNRSSGRQGYALAQAALDAGADVTLVTTPTALTPPIGARVIPVETAKQMLDAVTQEFPANDVLVMAAAVADFKPKKIAEDKIKKEGGIPQIELEATDDILKTVAGLRSGRKRKQVVVGFAAESQDLLQNAANKLQSKKLELIAANDISASDAGFSVGTNRLTLLFSDGRRESLSLMSKSEAAEIIVERVAALLE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.2. The designed protein should contain one or more Flavoprotein domains that are essential for its function.3. The designed protein should interact with enzymes and substrates within the coenzyme A biosynthesis pathway to enhance or regulate its activity.4. The protein must exhibit phosphopantothenate--cysteine ligase activity, metal ion binding. The designed protein sequence is ",
    "response#1": "LRDAGSDVRVVMTRGARAFVTPLTFQAVSGNPVRTELLDPDAEAGMGHIELARWADRILVAPATANFMARFAQGLADDLLSTLCLATTAPVALAPAMNRQMWAHPATQANCAVLEERGVRLIGPESGSQACGETGPGRMTEPAELVAALVEEARRIDEQAGA",
    "response#2": "LRDAGSDVRVVMTRGARAFVTPLTFQAVSGNPVRTELLDPDAEAGMGHIELARWADRILVAPATANFMARFAQGLADDLLSTLCLATTAPVALAPAMNRQMWAHPATQANCAVLEERGVRLIGPESGSQACGETGPGRMTEPAELVAAVEAL",
    "response#3": "KHILLGVTGGIAAYKAVELLRLLKKAGADVTVIMTESAKKFVGITTFEVLSENPVLSDLYFDTDASVKHIALAQKADLSVIAPATANTVAKLAAGLADNALTTTLLAVTCPVMVCPSMNTDMYQNIRVQRNLDKLEKDGWHILDPDSGVLACKTTGAGRLGSGA"
  },
  {
    "reference": "MPKKKEKILVLATRNPGKLRELKALLQDLDLEVKGLEEFPEAPEVIETGQTFFENAFQKAREIALATGHLAMADDSGLEVDALGGAPGVFSARYAGPKASDEENIKKLLKELEGVPLERRTARFRCVIVVYHPSGHWFKAEGTWEGLITLEPRGEGGFGYDPVFLIPDLGKTAAEIPPELKNQLSHRAKALAEMKYKLPSFLETL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP2. The substrate, Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein should be optimized for dITP diphosphatase activity, XTP diphosphatase activity, nucleotide binding, ribonucleoside triphosphate phosphatase activity, metal ion binding activity in order to improve purine nucleoside triphosphate catabolic process, nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "MMSKIILATGNAGKVRELAAMLEPAGVSVVPQSAFDVPECPEPAHTFVENALAKARHASRHTGLPAIADDSGICVEALGGEPGVYSARYAGEPKDDGRNNAKLLHELGDVPDARRTAHYVCVIVYVRHADDPQPIIAEGQWRGRILHEARGDNGFGYDPLFWVPXLGCTSAELPASRKNAISHRGQALAALLARLR",
    "response#2": "MSIILGIDPGSRITGYGVIRQVGRQLVYVGSGCIRTSSDDLPLRLKLVYAGVSEIITQFNPDCFAIEQVFMAKNADSALKLGQARGAAIVAAVNRDLPVFEYAARQIKQTVV",
    "response#3": "MTRIVLASNNAGKLREFQQLLGPLGIEVLPQSAFDVADAVEDGLTFVENALIKARHAARHTGLPALADDSGLEVDALGGAPGVYSARYAGEPKSDERNNAKLLHELAGVADHQARFHCVLVLVRHADDPQPIIAQGVWHGRILDWPRGEHGFGYDPHFYLPELGCTSAELDAAVKNQRSHRGRALRALLERLQAEH"
  },
  {
    "reference": "MSQFDNVSVVKEANVYFDGKCVSHGIIMPDGSRKSVGVILPAKLLFSTGAPEIMEILAGECKVTLAGETSASSYKAGDSFKVPGESSFQIEVSDTVHYVCHFG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Also catalyzes the reverse reactions.. The designed protein sequence is ",
    "response#1": "MKILGIETSCDDTAVSVYDSEEGLLSNVVSSQIKMHEEWGGVYPDLAAREHTKNIIPVLDRALKEASVNIKDIDGIAVTVAPGLIVSLVIGISVAKTLSWIYRKPLIPVHHIEAHIFASFITEKIDYPFIALVVSGGHTELYLIKGFEDYRYLGGTLDDAVGEAYDKVARMLGLGYPGGPVIDRLSKEGEDTVKLPRPLINDRGKNRFNFSFSGLKTAVLREIQKGVYRKEDIARSFQEAATDVLLAKTIDAMKEFNIKNVVIAGGVSANSRLREKFKEAEENHGIKAYFPPLYLCTDNGAMVAFTGYKRFKESGTTVDYSFEGKARLRMLPDAYKSVNEALKHAGLKKRLTVNIHYVDAQDVMVKGPHILKDLDAILVPGGFGERGTEGKMLAAQYARENKVPYLGICLGMQLALIEFARNVAGLTDAHSTEFNPDSPHPVVGLITEWLDAEGQVEMRNAKSDLGGTMRLGAQKCYLKPKSKAREVYGSEWIVERHRHRYEVNNNYIEQLEKAGLVFGGLSEDKKLVEMIELPDHPFYVAGQFHPEFTSTPRDGHPLFEGFIAG",
    "response#2": "LFAGFTEELLDKLVSSSRVVSFEQNEAIVHYGAEATHFGVILAGTVTASVIAGGGIRLELGRLEAGSTFGELALMTGEKTLTELIAATRCDVLLIPVSVFQSIIVAEPQVVQHISRTILF",
    "response#3": "MKILGIETSCDDTAVSVYDSEEGLLSNVVSSQIKMHEEWGGVYPDLAAREHTKNIIPVLDRALKEASVNIKDIDGIAVTVAPGLIVSLVIGISVAKTLSWIYRKPLIPVHHIEAHIFASFITEKIDYPFIALVVSGGHTELYLIKGFEDYRYLGGTLDDAVGEAYDKVARMLGLGYPGGPVIDRLSKEGEDTVKLPRPLINDRGKNRFNFSFSGLKTAVLREIQKGVYRKEDIARSFQEAATDVLLAKTIDAMKEFNIKNVVIAGGVSANSRLREKFKEAEENHGIKAYFPPLYLCTDNGAMVAFTGYKRFKESGTTVDYSFEGKARLRMY"
  },
  {
    "reference": "MTVHPAAYYRDILAELLMSSSGPMDRDISSVCADSRQVQPGALFCAIKGQKADGHDFIPAALAAGAAAIMLERPCALPPDVVYFQVKEPYRAMAVLAEAAAGFPARALRLIGITGTNGKTTSAYLLRHIFQQASCRPAMIGTVVYDLGDGSIRNADRTTPTPFELQELFSKIKSAGANPVVIEVSSHALAQYRLGTAACTATLFTNITRDHLDYHHDFEQYYQAKKLLFTIGRRPGTPMVINADDPYGRRLAEELDDKDALLPFTLHSAPLAKLRVEALTLAPEGGSFTIRDHHDSDVWRLRTPLPGLFNVYNAAGAAILADAMGVPRDIITRAIADCHGAPGRMQRVKEITRCAVFIDYAHTDDALANALGALRALQPKRLTVVFACGGNRDRSKRPLMGKVANDLADRIVVTSDNPRDEKPGDIIDQILAGINTPAKVRAIGDRREAIFQALDDAEAGDVILIAGKGHEDYQEIMGVKHPFSDEAVAQDWAEHKQSR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein contains novel Mur ligase C-terminal, Mur ligase N-terminal catalytic, Mur ligase central domains that confer a unique function or activity.2. A protein that can modulate the overall flux of metabolites through the peptidoglycan biosynthesis, Cell wall biogenesis pathway, enhancing or suppressing its activity as needed.3. The designed protein should contain at least one Meso-diaminopimelate recognition motif motif to achieve its intended function.4. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.5. We need a protein that can perform ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate with high efficiency.6. The designed protein should be involved in peptidoglycan biosynthetic process, cell wall organization by localizing to cytoplasm and exhibiting magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, ATP binding. The designed protein sequence is ",
    "response#1": "MTLDSREAGPGVLFVAVAGAHVDGRAFISQALASGVDAVLCDADTMPEDVPLQDARVLGVPGLRGRLVTGITADSRAVRTGYLFAALSGSKADGRAFIAQAVTMGASVILGAEGVELVTGTSGKTSTAWFTQGLFAATGHTAGYLGTLGLHVPGLPEQYTLTTPDPVALHLYLAQAADAGVTHFALEASSHGIDQRRLDGVRLKAAAFTNLQRDHLDYHATMAAYFEAKARLFLDLLPENGTAVVHAGDDWGQVLRDRVQQRGQTCLTYGTKTADLALTGRALAPGGQALTISALGETAEVIFPVLGSFQAENLLAALGVLLATGVPLAAACDALGRLQPVRGRMERIWSHDRHRCAVVDYAHTPDALQKALEALRTVTPAGAKLAVVFGCGGNRDRQKRPEMGRIAAELADRVILTSDNPRDENPEAILDEIEAGMAGRAHLRIADRAEAIRRAVGQLGEGDILLVAGKGHEPYQEIAGVKHPFDDADHAALALQRRTA",
    "response#2": "MKLNELISYAKIIETKGDYDIVEGITLDSRKVRPNWMFVAIKGLQSDGHDFINKAIELGADCIICEQIPSERHPHVTYVRVNDAAASLGVMAAEFYRNPAKKLKLIGVTGTNGKTTTTSLLEYLWSGSGLPAGVIGTLGARYHTQGKTVSLPSARTTPEASELQKNLALMRDSGVQVVAMEVSSHALSLHRVDGIYFELSLFTNLTQDHLDFHHTMEEYFLAKSALFTSKFSAGALVNADDSFGKRLLAQCEIPAISISTDKGDWQLSLTGFQLTRPGGAIHIIDSPLVGDFNRMNAAMALVEITHLSIDSRKTKRGSLFFCIKGYQMNGHQFAVEAEAKGAAAIVAEDRIDAGVPVVYVSDTRRALAMIADYYYNLPRNAIESALRSLSGVRGRMERIWSHDRHRCAVVDYAHTPDALQKALEALRTVTPAGAKLAVVFGCGGNRDRQKRPEMGRIAAELADRVILTSDNPRDENPEAILDEIEAGMAGRAHLRIADRAEAIRRAVGQLGEGDILLVAGKGHETYQIIGDKKIFFDDREIAIEALKNIK",
    "response#3": "MTLDSREAGPGVLFVAVAGAHVDGRAFISQALASGVDAVLCDADTMPEDVPLQDARVLGVPGLRGRLAALAPDCVGVTGTSGKTSVAAFTRQIWERQGLASASIGTIGLVTPQRTVYGSLTTPDPIALHRSIDEITGEGISHLVLEASSHGLDQHRLDGLRISAGGFTNLSRDHMDYHPDVAHYLAAKLRLFRDLVMPGGAAVISADHDCSAEAIDAARSRGLRVISIGRNGDPHDGIRLQSTAIDGFAQRLTITHQGRSHDILLPLVGQFQIENALVAAGLAIAMGEDLDAIADGVRALAGVPGRMERIWSHDRHRCAVVDYAHTPDALQKALEALRTVTPAGAKLAVVFGCGGNRDRQKRPEMGRIAAELADRVILTSDNPRDENPEAILDEIEAGMAGRAHLRIADRAEAIRRAVGQLGEGDILLVAGKGHETAQIFGDEVVPFDDRAVARAALERLRA"
  },
  {
    "reference": "MTPDELFRDIPHEVLREGDGRAITSVVIDSRKVGDGCLFVCLRGLKVDGHGFASEVAEKGAAALLVDRASDDYPAGLRVVRVDDTRKALSPLAANCCGRPADALRLFGVTGTNGKTTVTYFLEAILRQTGSVGLIGTLGARLDGVAADIPYETSTTPDPPELQRILAYMRDGGASDVVMEVSSHALALYKLAGIRFAVGVFTNITQDHLDFHGTMDNYLEAKTRLFGQCASGVINADDARAASVMASARCNWLTYGIENACDLRASNIEQLTDGSSFDVEIDGKEERFSIPARGRFNVYNALAAIGAALAAGVPITHIKNGLARAPGVPGRIQSVQNRKGAHVLVDYAHSPDGLANIINAVRDFTKGRVITLFGCGGDRDAAKRPIMGRVAGELSDYCIVTSDNPRSEAPDEIIRQIVAGLSETSCPFETLPDRRAAIFRGAAMAGAGDALIIAGKGHERTQIIGGEVLPFDDVEVAREALSE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm that is critical for peptidoglycan biosynthetic process, cell cycle.2. A protein that can modulate the overall flux of metabolites through the Cell wall biogenesis, peptidoglycan biosynthesis pathway, enhancing or suppressing its activity as needed.3. The protein should be able to bind meso-2,6-diaminoheptanedioate ligand in a variety of conditions4. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.5. The designed protein should contain one or more Mur ligase C-terminal, Mur ligase central domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MKISRLACDSRAVRPGDTFVAYPGETRDGRDFIAEAIARGAVSVLWERKGYAWDARCKVPNLSVRNLRRYAGEIASAWYGEPTAALRTIGITGTNGKTTVTQLINQWSTILGTKTATMGTLGNGFYNSLQPTNNTTSSPIFIQLFLSKVLEKQAELVTMEVSSHGLIQHRVKEVPFYIAIFTNLTQDHLDYHENMEKYESAKWLLFSTHKVKKIILNADDQYGKIWLKKLLNFYTVAVTIQNRKQKKYSTKWINATNIEQNNNNSIYITFESSWGTGRISSCLIGHFNVTNLLLSLACLHAAGYAFEDLARICPALKAPAGRMQRVGGGAKPLVVVDYAHTPDALEKVLRSLRELMLHRSPVTGHPSRLICVFGCGGDRDRGKRPQMGRIAARLADSVVVTSDNPRSEDPHAIIVDILEGARRGKRELCVNADRRDAIEAAIAGAQRGDIVLVAGKGHEDYQEIKGVKYPFSDVEHARQALDRRLARG",
    "response#2": "MRLVTGTNGKTTTARLIASILSMNGLPCGYVGTGKAIVGGREFPLERTTPEGHGLQRLLRMMVDAGSRAVVMEVSSHALELGRVHGLAFDAAVFTNLTPDHLDFHPSMEAYAEAKRRLFDQLAPGGMGVFNVDDPKAAFMAARVPEDQRACCSLEAHPFRGMRCGSSYGALLAGESAGSSRVRLSFPGVAELEAEYRLPGRFNVMNLLEAAALALQEGTEEDAILSAIRAFKGAPGRFEYIQAPSGVTVVIDYAHNPDAIFQVLSCLNVISTGNIITVLGAGGNRDKAKRKIMGRHATYLSDHVFFTSDNPRNEDPLSIIYDLLSGTSSVNYTVIVDREKAIEAALKKAKPNDFVVILGKGDEAGQYAASMISGRMEAVGEGQEFQVLVDYAHTPDGLENALAAVKEVTEGRVITVFGCGGDRDRTKRPIMGKIAARFSDYVFVTSDNPRSENPEAILRDIEPGVLEAGFAAENYQLIADRREAIQKAIEMASPKDVVLIAGKLQGL",
    "response#3": "MKLFNGVSSDSRSIKKGNIFFAIPGNEIDGSRFXNQAIKKGAAAIVVPSYXKERYPKNTTVIKAKDIRDALSIIAKEFYGTNGKTTITYLIQHVFECLGIDSGLIGTNGSWVGQKKKASRTTPLSPDLNELLQEGVRHNLPYVVMEVSSHGIKENRVDQLDFDRLIFTNITEEHLDYHKTFEDYLFTKMRPFIQFSDYDDKKMAIINCDDEHASSFISVTNSKILTYGCQAKADIFAKDIEYSLERTNFSLYVKGQFICQMTIPLFGKYNVYNVLSVLVTGTNGKTTVSTLLYQLFKKAGFKVGLLSTIRIMVDDKEYDTLHTTPDALTINYHLSLMSEEGVEYCFMEASSHGIDQKRTEGLVFAGAIFTNLSHDHLDYHKSFADYRDAKKALFDHLPKTAFALSNMDDKNGLVMLQNTKARKFTYALKSYADYRAQILENQFSGQLLKIDNNELWTKLIGNFNAYNVLAIYATADLLGMEKVEILRLISELESVSGRMERVEGPAIRGTAIVDYAHTPDALRQALSALRALLPPDGRLVVVFGCGGNRDRLKRPEMGRIAAELADRVVITSDNPRDEDPEAILDGIEAGMEKRGHLRITDRAEAIRAAVAMLRRQDVLLVAGKGHETYQEINGVRNHFSDVEQASAALKR"
  },
  {
    "reference": "MSKSASRARLLEIIRRRSFGRGEVTLASGRKSDFYFNLKPTMMDPEGATLLAELTYEALKDDGYDYIGGLEMGAVPLAGAIAQISWIKGHPIAAFFVRKKPKEHGARLAIEGLTRDETLAGKRIVVVEDVTTTGGSAMKAVETLREAGAEVSLVFTMVDREEGAAEAFAAAGLSFRALYKAREFL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must have high specificity towards orotate phosphoribosyltransferase activity.2. The designed protein should interact with enzymes and substrates within the Pyrimidine metabolism pathway to enhance or regulate its activity.3. The protein should be able to bind orotate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "TEKLCRGLAQEIKRHVEGKIDYVVGPAIGGLIPSYETSRHLGVPSIWVERENGVFRLRRFEVEKGARVVIVEDIVTTGLSIRETIEALVAVEADVVASACIIDRSGGKR",
    "response#2": "MLWEIKAVHFRADEPYRLASGMASPVYIDCRKLVSYPRIRSAVMDFAAATILREAGFEQFDVVAGGETAGIPFAAMLAERLGLPMIYVRKAPKGHGRNAQIEGYMPEGARVLVIEDLTTAGGSMFKFIDAIRAAGGVVEHGIALFYYDIFPEARGNMKSKGVDLHYIS",
    "response#3": "MDFAAATILREAGFEQFDVVAGGETAGIPFAAMLAERLGLPMIYVRKAPKGHGRNAQIEGYMPEGARVLVIEDLTTAGGSMFKFIDAIRAAGGQ"
  },
  {
    "reference": "MKKYRVAILGATGAVGQEFLTLIEERKFPFSELRLLASSRSAGKKIAFMGKEYTVQETTPDSFEGIDIALFAGGAASKEFAPHAVKAGAIVIDNSSAFRMDPEVPLVVPEVNPEAIKAHKGIIANPNCSTIIMVMALKPLYDIAKIRRIVVSTYQAVSGAGKEGMAELEEQVAALASGKEPVAKVLPVGKLPKHYQIAFNLIPQIDVFKDNLYTKEEMKMIDETKKIMSDDRMRITATTVRVPVYRSHAESVNVEFEDKVTLEAARAAIAAFPGIILRDNPAEQEYPMPLFTSNKYDVEIGRLRYDESQENTLNFWICGDQIRKGAALNALQIAEYMIAHDLV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Acyl-thioester intermediate, Proton acceptor of the protein must be engineered to enable effective catalysis of L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.2. The designed protein should have a binding site that is specific to the target NADP(+) ligand.3. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.4. The protein design must be able to perform NAD binding, NADP binding, protein dimerization activity in order to contribute to 'de novo' L-methionine biosynthetic process, lysine biosynthetic process via diaminopimelate, isoleucine biosynthetic process, diaminopimelate biosynthetic process, threonine biosynthetic process.5. The protein design should consider the endogenous proteins and enzymes present in the L-threonine from L-aspartate: step 2/5 pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MKVAILGATGMVGQKFVQLLSNHPWFKITALAASERRIGKKYGDEVNWLMSAEIPESVRDLELVPMNPKYIDADIVFSALPADVAREIEPLFAKEGFIVASNASAFRMEEDVPLVIPEVNPDHLGLLKPIHDAVGITRINVATYQSVSGAGQQGIEALARETTSLLNGRPIEGSGKFNRQIAFNVIPQIDVFMDNGYTKEEMKMVWETRKIFGDDSIMVNATCVRVPVFFGHAEAVHIETASPISVEKVKDLLRDAPGVVLMENDADFPTQVGDAQGQDAVYVGRIRQDISHPCGLNLWIVADNARRCKIVAGTCVRIPVFTGHLKPIYDAVGISRINVATYQSVSGTGKEAIIELTEQTKQLLANEPIQPKVYSQQIAFNVLPHIDDFQENGYTREEMKIVWEMQKIFADTTLAINPTAVRVPVFCGHAAAVHLETKDKLTAKQALELLSHAPGVKLITGDYPYATPARDAAGKDFVYVGRVREDISHENGLSLWIVADNLRGCATTIDLAPLHQRWSITRLSLSTYQAASGGGAEMMEELREGTRAHLNGEDFEPQILPHSYAFNVFSHNSDIDPETGYNGEELKVIAETRRILSQPQLPISVTCIRVPILRAHGISLSIRFDEHVNIDSIRHALVGAPGIRLVDDREANHFPMPSEASGEQDVLVGRIRDDLGDPSGRTVSMFIVGDQLLGV",
    "response#2": "MNVVAIVGATGAVGLALIHSLEQRNFVVGALRLLASARSAGRSQSFRGQSIVVEELTEDSFDGVDIAFFSAGATISRRFAPIAVAAGAQVIDNSSAFRMQDDVPLIVPEANGAALQSTKLVAGTCVRIPVFTGHPLLKALKPIYDAVGISRINVATYQSVSGTGKEAIIELTEQTKQLLANEPIQPKVYSQQIAFNVLPHIDDFQENGYTREEMKIVWEMQKIFADTTLAINPTAVRVPVFCGHAAAVHLETKDKLTAKQALELLSHAPGVKLITGDYPYATPARDAAGKDFVYVGRVREDISHENGLSLWIVADNLRKGAALNALQSLQLLEEHHGWL",
    "response#3": "MRIAVVGATGLVGQKILEILEERNTPVRSLVALATEGHGRFIEFRGQQVPVQSVDAAHWDEIDVAFFAATNDVSRHYAPLATAHGVTVIDKSSYFRMDPTVPLVVPEVNAEVIGDALARDKGIIANPNCSTIQMVVALKPLHELFKIRRVVVSTYQAVSGAGKTGMDELFTQTRGSFVHDPIEAKQFTKQIAFNVIPHIDRFLEDGSTKEEWKMAVETRKILDPDIQVLATCVRVPVFIGHSEAISIECERPIDLRKAQEALRHAPGVILRDEREDGGYVTPVECVGEDDTYVSRLRIDPTVENGLAFWCVSDNLRKGAALNSVQIAELLLKPLHDAFKLKRIIYSTYQAVSGSGVKGVADLENGTTDNYPYPISYNCLPHIDSFLENGYTKEEIKMIDETKKILNDYDLKITATTVRVPVKNGHAVSINLEFEKPFELSEVFELLKNAPGIVVQDDPANLVYPMQIEVSGKDEVYVGRIRRDFSVENGLNLWVVADNIRL"
  },
  {
    "reference": "MNFNNNNNNNNNNNNNNNLNNNNNNVNNINNNNKKPLTNSLAGTPPAKKILVIKNLKQIPKTPDNYEDSSWNKLSSAITSINMKQATTLTQEELYKMVENLCFDKILASNLYNKISVQIEKHITLTIKHLVLTMSSDPIIFLKSINSIWKDHTNQMIMIRSIFLYLDRTYVIQNSNTVKSIWDLGLFYFGNNLSQQSNLERKTIDSLLYSIRCEREGDEIDRDLIHSLVKMLSSLNIYTKFEIEFIKETNRFYDMEGNSKINEIETPMYLKYVCERLNQEGERLMRYLEQSTKKQLMAVLDRQLIERHVDVILEKGFNAMVNGDRLEDLGKLYQLLNSVGEIKKIKESWQSYIKQTGIQMLNDKEKEATLIQDLLDYKDRLDRILSQSFSKNELLTYALKESFEYFINTKQNKPAELVARFIDSKLKVGGKRMSEEELETVLNKSLILFRYIQGKDVFEAFYKQDLSKRLLLDKSTSIDAEKSMISKLKTECGTTFTAKLEEMFKDIELSNDIMNSFRDSPMTQNFKSIEMNIYVLTSGNWPIQPPIEATLPKEFLEYQEVFNKFYLSKHNGKTLKWQNALSYCVLKANFIQGKKELSVSLFQTIILYLFNDVIDGGELSFRDIQANTGLAIPELKKNLLSLCSSKSDILIQKKSSTSSNTSSNTSSNTSSSASGSASGGASGGATKTKVIDETDTFLFNSKFSSKLFKIKVNSIQIQETVEENQKTNENIISDRQYQVDAAIVRIMKTRKTLAHNLLISELVSLLKF",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that is capable of catalyzing reactions within the Protein modification pathway with high efficiency and specificity.2. A protein that can perform protein-macromolecule adaptor activity in cullin-RING ubiquitin ligase complex, nucleus, Cul4-RING E3 ubiquitin ligase complex to support protein ubiquitination, SCF-dependent proteasomal ubiquitin-dependent protein catabolic process, cellular response to DNA damage stimulus.3. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.4. For general function, the protein need meet that Probable core component of cullin-based SCF-like E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins The designed protein sequence is ",
    "response#1": "KLQRILEDLSEPPFDPGQYINLYTIIYDMCLQQPPNDYSQELYNKYRGVVDHYNKETVLPSMRERHGEYMLRELVKRWANHKILVRWLSRFCFYLDRFYVARRGLPTLNDVGFTSFHDLVYQEIQSEAKDVLLALIHKEREGEQIDRTLVKNVIDVYCGNGVGQMVIYEEDFESFLLQDTASYYSRKASRWSQEDSCPDYMLKAEECLKLEKERVTNYLHSTTEPKLVEKVQNELLVVVAKQLIENEHSGCLALLRDDKMGDLSRMYRLYRLIPQGLEPIADLFKQHVTAEGNALIKQAADAATNQDASASQVLVRKEIELHDKYMVYVDECFQKHSLFHKLLKEAFEVFCNKTVAGASSAEILATYCDNILKTRGGSEKLSDEATEITLEKVVNLLVYISDKDLFAEFYRKKQARRLLFDRSL",
    "response#2": "KLQRILEDLSEPPFDPGQYINLYTIIYDMCLQQPPNDYSQELYNKYRGVVDHYNKETVLPSMRERHGEYMLRELVKRWANHKILVRWLSRFCFYLDRFYVARRGLPTLNDVGFTSFHDLVYQEIQSEAKDVLLALIHKEREGEQIDRTLVKNVIDVYCGNGVGQMVIYEEDFESFLLQDTASYYSRKASRWSQEDSCPDYMLKAEECLKLEKERVTNYLHSTTEPKLVEKVQNELLVVVAKQLIENEHSGCLALLRDDKMGDLSRMYRLYRLIPQGLEPIADLFKQHVTAEGNALIKQAADAATNQDASASQVLVRKEIELHDKYMVYVDECFQKHSLFHKLLKEAFEVFCNKTVAGASSAEILATYCDNILKTRGGSEKLSDEATEITLEKVVNLLVYISDKDLFAEFYRKKQARRLLFDRSS",
    "response#3": "MRAAVDALELRLRTDAAYFRALYAYTFGLGLDAGGRRLSVETAIAYWSLFFLDHTYAVTVPAPRLRSWFEFLRAGDHSVSRDTWDMFPRFAQRFPDDTELLEHYNELASWPLVIDEYYED"
  },
  {
    "reference": "MASQLRLRSALALVTGAGSGIGRAISVRLAAEGAAVAACDLDGAAAQDTVRLLGSPGSEDGAPRGKHAAFQADVSQGPAARRLLEEVQACFSRPPSVVVSCAGITRDEFLLHMSEEDWDRVIAVNLKGTFLVTQAAAQALVSSGGRGSIINISSIIGKVGNIGQTNYASSKAGVIGLTQTAARELGRHGIRCNSVLPGFIATPMTQKMPEKVKDKVTAMIPLGHMGDPEDVADVVAFLASEDSGYITGASVEVSGGLFM",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Prefers (3R)-3-hydroxyacyl-CoA over (3S)-3-hydroxyacyl-CoA and displays enzymatic activity only in the presence of NAD(+)(H); NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol.2. The protein must have high specificity towards testosterone dehydrogenase (NAD+) activity, 17-beta-hydroxysteroid dehydrogenase (NAD+) activity. The designed protein sequence is ",
    "response#1": "LVTGGAGFVGANLVTTLLDRGHWVRSFDRAPSLLPAHPQLEVLQGDITDADVCAAAVDGIDTIFHTAAIIELMGGASVTDEYRQRSFAVNVGGTENLLHAGQRAGVQRFVYTSSNSVVMGGQNIAGGDETLPYTDRFNDLYTETKVVAERFVLAQNGVDGMLTCAIRPSGIWGNGDQTMFRKLFESVLKGHVKVLVGRKSARLDNSYVHNLIHGFILAAAHLVPDGTAPGQAYFINDAEPINMFEFARPVLEACGQRWPAA",
    "response#2": "LVTGAGGFLGQRIIRMLAQEKELQEVRTLFRSFTPKHREELSKLQTKTKVTVLEGDILDAQCLRRACQGISVVIHTAAAIDVFGAIPRQTVIDINLKGTQHLLDACIGARVPVFIYSSSVAVAGPNSYKVIIQNGSEEENHESTWSDPYAYSKKMAEKAVLAANGSTLKDGGTLHTCALRLPFIYGEKSKFISDTMDRALKNNGLINGFSRFSVISSVYVNNAAWAHVLAARGLQDPKKSPNIQGQFYYISDDTPHQSYDDLCYTLSKDWGLRPDSSWL",
    "response#3": "LVTGAGGFLGQRIVQMLVQEKELQEVRVLYRTFSPKHKEELSKLQTKAKVTVLRGDIVDAQFLRRACQGMSVIIHTAAALDIAGFLPRQTILDVNVKGTQLLLDACVEASVPAFIYSSSTGVAGPNSYKETILNDREEEHRESTWSNPYPYSKRMAEKAVLAANGSILKNGGTFHTCALRLPFIYGEESQIISTMVNRALKNNSIIKRHATFSIANPVYVGNAAWAHILAARGLRDPEKSQSIQGQFYYISDDTPHQSYDDLNYTLSKEWGFCLDSSWSLPLDXLX"
  },
  {
    "reference": "MAGSTTIIASLLLLVACTCCICPTGSVTIPSSCCVTFISKKIPVNRVISYQLANGSICPKAGVIFITKKGHKICTDPKLPWVQKHIKNLDAKRNQPSEEPCLPVCCRAPALTTMD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Ensure the presence of a signal peptide within the protein.2. The designed protein should be optimized for CCR3 chemokine receptor binding, chemokine activity activity in order to improve signal transduction.3. The protein contains novel Chemokine interleukin-8-like domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "TVRCNCISISKHVNPRFIETLEIIPASQSCSRVEIIATMKNNGEKRCLNLESKAIKNLLTAVSR",
    "response#2": "ACCFSYISRQIPYKFIADYFETSSQCSKPGVIFLTKRGRQVCADISEAWVQGYINDCL",
    "response#3": "RCQCIKTHSKPFNPKLIKEMRVIESGPHCENSEIIVKLVNGAEVCLNPHTKWVQIIVS"
  },
  {
    "reference": "MSRIGNKVITLPAGVEVSQEGTVVTVKGPKGTLSREIASQISMSVEGTEVAFTRESDDNKTKALHGTTRANVANMVEGVSEGFTKTLKLVGVGYRAAKSGSKLTLSVGYSHPVDFEDREDLTVEVPDALTVKVSGISKQRVGDLAAEIRAVRSPEPYKGKGIRYEGEVVRRKEGKTGK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that This protein binds to the 23S rRNA, and is important in its secondary structure; It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.2. The designed protein should have rRNA binding within ribonucleoprotein complex to regulate translation. The designed protein sequence is ",
    "response#1": "PRKGPAPKHPVVVDPVYGSPLVTALVNKVLLSGKKSVAERIVYGALEGAKNKTGNDPVVTLKRALDNVKPTLEVRSRRVGGATYQVPVEVRAGRSTTLALRWIVGYSRGRREKTMTERLMNELIDASNGLGASVKRREDTHKMAESNRAFAHYRW",
    "response#2": "MPTILN",
    "response#3": "PRKGPAPKHPVVVDPVYGSPLVTALVNKVLLSGKKSVAERIVYGALEGAKNKTGNDPVVTLKRALDNVKPTLEVRSRRVGGATYQVPVEVRAGRSTTLALRWIVGYSRGRREKTMTERLMNELIDASNGLGASVKRREDTHKMAESNRAFAHFRW"
  },
  {
    "reference": "MAEILTGGQMMALERAAMARGAAESGVLMDAAGRAVFDTIFETWPELHAAPQRAVVLCGPGNNGGDGYVIARLLREWGWEVEVLAFGAPGDGAADAARNAARWHDMGGASRALDGGALEEALPGAALCIDALFGTGARALSGDLAAWVDRLNVLAAAEGALKIVAVDLPSGVSADSGKAPGGAVAADLTVSFHRAKLGHYLADGPAAAGALRDCPIGLPDDPCADAVMRIAPPTAADLLKTSGHKYDYGHALILAGPPGQGGAARMAARGALRIGAGLVTVACPQRAVYENAAQLDAIMLRGIDGPSGFEELLEDARLSALCLGPGLGLGAITAQLVRVALEAGRPTVLDADALTRFSREAERLFDMAHPDCVLTPHEGEFARLFPDLAEEMRDAATETSRADALRKAADRAGCTVLLKGPATLIASPGGDCRLHSALYDEAAPWLATAGAGDVLSGFITGLLARGFAPLDAASNAVWLHTACARNFGPGLIAEDLPEQLPAVFRTL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration2. The designed protein must have at least one YjeF N-terminal domain.3. The designed protein must possess kinase activity, ATP binding, metal ion binding to support phosphorylation under certain conditions.4. The designed protein should have a high specificity for (6S)-NADPHX binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "DVGYAWRVPGPRDDKYTQGVTGIAAGSANYPGAAVLSSGAAVLATSGMVRYAGYAADVVRGRWPELVATGNVEDAGRVQAWVVGPGLGTGQAGRSILAAVLHEAVPTCVDADGITLLAANPALWDARDPDAPVVLTPHDREFARIAEPLGIKLGADRVAAARELAKRLDSVVLLKGNATVVAAPDGRTLVNPATSSWLATAGSGDVLTGLVGALLATGLDPWLAAGCAAWAHVRAGELAATADASATADEETGVPTPASRILEAIPGAIRAARA",
    "response#2": "MKKAEAMCEQKGTSCAVLMRNVGSAIALHISRIVKPCRAAVLVGSGNNGGDGFAVAHNLRKRGFSPLIVLVGSAPKTDLAIDCFNEYKPDYEAVLSYPDQPETVLSELGSCGIIIDCVYGTGFHGELAPPVRRLFSYCNGSAALRFCADIASGCNATDGNADEYSFRADMTFALGAVKTGQLYVPCSEFSGDIVLLDIGISEACFD",
    "response#3": "MKKAEAMCEQKGTSCAVLMRNVGSAIALHISRIVKPCRAAVLVGSGNNGGDGFAVAHNLRKRGFSPLIVLVGSAPKTDLAIDCFNEYKPDYEAVLSYPDQPETVLSELGSCGIIIDCVYGTGFHGELAPPVRRLFSYCNGSAALRFCADIASGCNATDGNADEYSFRADMTFALGAVKTGQLYVPCSEFSGDIVLLDIGISEACFLPVPGAESDKYRRGVVGIAAGSARYPGAAVLAVAGALRGGAGAVRYVGAAGEAVLARFPETLVSDRGPAKAGRVQAWVVGPGAGEDAGPVAEVLAAEVPVLLDADGLRLAERDAVRARTAPTLLTPHAGEAAALLGVSREEVEGARLTAVRELAARYRATVLLKGSTTLVAGEPGEPGEPEAPVRVNATGTPWLATAGSGDVLSGLAGSLLAAGLSARDAGSTAAHLHGLAGRLAARGAPVGAHDVAEAIPRAWLGG"
  },
  {
    "reference": "MAQRVLLIVSGGIAAYKALELVRLLRGRDIAVRAVMTEAAGQFVTPLSLGVLTEDHVYGALFDLKDEREIGHIQLSRDADLIVVAPASADILAKMAAGIADDLATTLLLATDKPVLAVPAMNVRMWHHKATQRNLAQLREDGIHVMEPAVGAMACNEWGKGRLPEPVGILAEIERLLAEGPGAAEPHPLLTDTTRTINAPDLSEHNLFGRHVLVTAGPTHEPIDPVRYIANRSSGRQGFAIAAAAARVGARVTLVAGPVTLPTPPGVTRVDVETAREMAAAVEQALPADVAIMVAAVADWRATDEAAQKLKKDGSGKPAPLALTENPDILATLGQHPQRPTLLIGFAAETETIIDHAQAKRARKGADWIVANDVSGNVMGGDSNAIHLVTADGTETWPDMPKTAVAMRLIERIADALAGR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design must be able to perform metal ion binding, phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity in order to contribute to coenzyme A biosynthetic process, pantothenate catabolic process.2. The protein should have a high affinity for CTP. The designed protein sequence is ",
    "response#1": "KNIVLGVSGGIAAYKSAELLRLIKKQDAGARVIMTKNASAFVGSLTFEALSGNPVCTDLFEKEGNSSIRHIEWAKEADAVVVAPATANIIGKIAGGIADDALSTFLLAVTCPVMICPSMNTNMYEKVSVRRNIDILKKDGYFIVEPSEGELACGTTGYGRLPEPEEILDRLL",
    "response#2": "GRRILLVIGGGIAAYKSLELIRRLRERGATVRAVMTPAACQFITPLSVGALTADRVFTDLFDREDEHDVGHIRLAREADLIVAAPATADLLAKMANGLANDLASTILLAAKVPVLVAPAMNPAMWQHPATRRNVAQLAGDGVAFVGPNSGAMAEAGEAGEGRMAEPLEIVAAIERRLSVASGPLAGKSVLITSGPTHEPIDPVRYIANRSSGKQGHAIAAAAARAGARVTLVSGPVTIPDPAGVRVLRVETAREMLQAVEAALPADVAIMAAAVADWRVASGSGQKIKKDGSGKPPALSLAENPDILATVGTLAEGRPCLVVGFAAETQNLIDNARAKLQRKGADFIVANDVSPETGVMGGDANTVRLVSRDGVEDWPTLSKEDVAERLVAVIAAKLADSGL",
    "response#3": "GRRILLVIGGGIAAYKSLELIRRLRERGATVRAVMTPAACQFITPLSVGALTADRVFTDLFDREDEHDVGHIRLAREADLIVAAPATADLLAKMANGLANDLASTILLAAKVPVLVAPAMNPAMWQHPATRRNVAQLAGDGVAFVGPNSGAMAEAGEAGEGRMAEPLEIVAAIERRLSVASGPLAGKSVLITSGPTHEPIDPVRYIANRSSGKQGHAIAAAAARAGARVTLVSGPVTIPDPAGVRVLRVETAREMLQAVEAALPADVAIMAAAVADWRVASGSGQKIKKDGSGKPPALSLAENPDILATVGTLAEGRPCLVVGFAAETQNLIDNARAKLQRKGADFIVANDVSPETGVMGGDANTVRLVSRDGVEDWPTLSKEDVAERLVAVIAAKLADSGR"
  },
  {
    "reference": "MAAGTTTKERLERLINAKKQLEAQINRNGQILAANDNVGMSGPLVDAEGFPRNDIDVYQVRLARQTIICLQNDHKELMNQIQTLLNQYHSEIATTDPELVNRASALDLDSDRSPGGANITDLAPARAIVVVNLVSPDSPAERAGLCAGDAILRFGSINSGNFKGDLAQIGELVRNMQSQNVQLKVKRGEQQLDLILVPKTWSGRGLLGCNIVLPPEAMDH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must possess metalloendopeptidase activity to support proteasome regulatory particle assembly under certain conditions. The designed protein sequence is ",
    "response#1": "VEKAELRLAIADSPQKFETNLQTFLPPLLLKLASPHASVRTAVFSALKNLISRINTLPQVQLPVRALIVQAKEPNLAAQQDSTNVRLYSLLLASKGIDRLSLQDRQQLLPLVVSNISCLTGTVAARMFHILLKLILEWVAPQESSHEQEEFVQFLQLDNDGFSFLMRQFTRFFLLVPSKQVQVSQQPLSRGYTCPGLSLTDVAFFTYDAGVTFNKEQLNKFKKAIFQFVCRGMAATQTIEQSPRMIELMEFLCVVSTDSTNLSDDAAQFMKRFPMPYENEEFITFLQTLYIGNTANGRPPVKAILQEKILSILNRSHFATTKAECISLICSIGLHSSEYKLRSLTLSFIRHVAKLNYKNLNPASSSPSSTDFSTCIVSLIRNNLHAEGWPKLQLGPQTPAFNTAILQRQLQYETLGDILKRDFELVSDLSYIEFLFESLKNDLPQFRSSIQESLLSLVGHLSILPQQSKLKLKNLLRKNLSIDEQQREDNNDAVNSIMALKFVSIKFTNAAFPFHDPEARLFNIF",
    "response#2": "VEKAELRLAIADSPQKFETNLQTFLPPLLLKLASPHASVRTAVFSALKNLISRINTLPQVQLPVRALIVQAKEPNLAAQQDSTNVRLYSLLLASKGIDRLSLQDRQQLLPLVVSNISCLTGTVAARMFHILLKLILEWVAPQESSHEQEEFVQFLQLDNDGFSFLMRQFTRFFLLVPSKQVQVSQQPLSRGYTCPGLSLTDVAFFTYDAGVTFNKEQLNKFKKAIFQFVCRGMAATQTIEQSPRMIELMEFLCVVSTDSTNLSDDAAQFMKRFPMPYENEEFITFLQTLYIGNTANGRPPVKAILQEKILSILNRSHFATTKAECISLICSIGLHSSEYKLRSLTLSFIRHVAKLNYKNLNPASSSPSSTDFSTCIVSLIRNNLHAEGWPKLQLGPQTPAFNTAILQRQLQYETLGDILKRDFELVSDLSYIEFLFESLKNDLPQFRSSIQESLLSLVGHLSILPQQSKLKLKNLLRKNLSIDEQQREDNNDAVNSIMALKFVSIKFTNAAFPFHDPEARLFNIV",
    "response#3": "EIMAPPGMEDMTSQLQNMFQNMSSEKTNKRKLKIKDALKALQEEEAAKIVNQDDIKQKAIDAVEQNGIVFIDEIDKICKRADSSGGGDVSREGVQRDLLPLVEGSTVSTKHGMIKTDHILFIASGAFQMTKPSDLIPELQGRLPIL"
  },
  {
    "reference": "MTESGSITLRAACEGLSAKFIGPSHVRLTSATVNSSAVEIGGLFGCLTGATVDGHDFIGQAIDNGAVALLVEKSITEFDVSKVAVALVEDTRRAIGPISSRIYGEPSRSLSCFGVTGTNGKTTTVHMLASIARAGDEKVGTVGTLGANFSLKGQDREKLSLSGLTTPEAPALQDQLAQMQQAGVTTVAMEVSSHALSQFRIDGTQFEMVCFTNLGHDHLDFHGDMDSYLLAKTRLFTPVFSDRAAINIDDSSGVHVANAAKVNGLSVTTFGLSPEADITATAIEYSISGTNFVITDRRTDESVEVSTNFLGEINVLNALAAAAMAQGGGHSLAISSTGLADATVVSGRLERIENTEPFTVLLDYAHTPDALAAALRAARTIAFESRVLLVFGCGGNRDFEKRPAMGLAAVAGAEVVVITSDNPRSEDPIEIANQITAKIDKSSVSVELDRRAAIRQALTAARPGDVVVIAGKGHETSQIFRTATVTHDDRVVIQEELARL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must have high specificity towards magnesium ion binding.2. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.3. Ideal protein should have a binding affinity for key enzymes in the peptidoglycan biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "IRQSIAEGFEQLIRSHFPEVTVVAGTATAGIPHAAWVSDRLQAPMVYVRSKSKGHGKQNQIEGKLSSKDRVVIIEDLISTGGSVIQAADALREAGADVLGVVAIFTYGLEKGQDC",
    "response#2": "IAEGFEQLIRSHFPEVTVVAGTATAGIPHAAWVSDRLQAPMVYVRSKSKGHGKQNQIEGKLSSKDRVVIIEDLISTGGSVIQAADALREAGADVLGVVAIFTYGLEKGQDQLEAANLPSYVL",
    "response#3": "VTGTNGKTSVSQLVAQALDLLGERCGIVGTLGTGFYGALESGRHTTPDPLAVQATLARLKQAGAKAVAMEVSSHGLEQGRVAALGFDVAVFTNLSRDHLDYHGSMEAYGAAKAKLFAWQGLRCRVINLDDDFGRQLAAESHESRLMGYSLSDPSAYIYCREATFSDAGVQAQIVTPQGEGLLRSSLLGKFNLSNLLAVVGDIAIFTNLSQDHLEYHHTMEKYAEAKSLLFSQLGNGYQQGKAKFAILNFDDPVGKSFEEKTAARVFSYGIDQPADFKATDLSITNKGTRFNLNFENRVYEVQLQLVGKFNVSNALAAIAGAYAAGLEVSSVIQSIGEISGVRGRFELVKGDQDFAVIVDYAHTPDGLLNVLNAVNEIKTGNVFCVVGCGGDRDRTKRPIMAEIAQKNSDTVIFTADNPRTEDPIAILDEMVEGLS"
  },
  {
    "reference": "MQRTSRLKRELSLLAAEPPPGITCWQDGDRMEDLRAQILGGANTPYEKGVFKLEVHIPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATLLTSIQQLMAEPNPDDPLMADISSEFKYNKPVFFKNARQWTEKHARQKTDEEGMPGSLPEVGGSEGPSAAQKRKAGQLSSGGKRFCPDV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The Glycyl thioester intermediate active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The protein contains novel UBC core domains that confer a unique function or activity.3. The ideal protein would be one that plays a critical role in protein K29-linked ubiquitination, protein monoubiquitination, protein K27-linked ubiquitination through ATP binding within nucleus.4. For general function, the protein need meet that Catalyzes monoubiquitination; Also mediates monoubiquitination of FANCL and FANCI The designed protein sequence is ",
    "response#1": "LTTAAPGYTCPLQGSHAICTCCFQPMPDRRAEHEQDSRIAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLTTRGLTWKNMLTESLLALQRGVFILSDYRITGNTVLCYCCGLRSFRELTYQYRQNC",
    "response#2": "LTTAAPGYTCPLQGSHAICTCCFQPMPDRRAEHEQDSRIAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLTTRGLTWKNMLTESLLALQRGVFILSDYRITGNTVLCYCCGLRSFRELTYQYRQNC",
    "response#3": "LTTAAPGYTCPLQGSHAICTCCFQPMPDRRAEHEQDSRIAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLTTRGLTWKNMLTESLLALQRGVFILSDYRITGNTVLCYCCGLRSFRELTYQYRQNC"
  },
  {
    "reference": "MFPYYPLRLLKSLQLLVWASVLLALTFIFSIFSISVTNVLSISFLRIPFALFGWIFGPIWGFIFGFLSDTIDWLTKGYVWFWMFALQKPLFAFLAGIVKGIYLVRKNASNYKVDFWVLQTLLVTFFVVSVTLLLLYLTNNEFQQIGTKNFQTADLSVNVVVLQIITLVSFVIFFLVTEGFLGFVYVKKRNKEQALLTIYSLVLMVLMSVVVSILLGTIAAIEFITFINNGRPSNNFVLYGVYFFLLPRVLVQALLLPIYVALFYPLIGIVENNLKNYLLLFTLSWKS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must be able to transmembrane transporter activity. The designed protein sequence is ",
    "response#1": "QSFWLITGIIFIAFNLRPAITSVGPVISSIRAELHMSNGAAGFLTALPLLSFAVLSPLAPKLGQRLGNERTLWLGLVILLIGVLTRSTGYTAALFFGTALIGVGIAIGNVLLPSLIKHKYPEKPGIMISLYTTSMNIFAALASGVSVPLATQMNGGWKQAFLLWGGLALLALLIWIPQLRHRDTANQTMKLQSSSIWASKMAWYVTIFMGLQSFLFYSSIAWFPEILRSHGIDTATAGWMVSLMQFASLPSTFLTPVLADRVKQQRGIVAALASVYLIGLCGLLAGGSHTLLAIWMIIIGIGQGSSISLALTLIGLRSENAQQAAALSGMSQSFGYLLAAVGPIFVGYLFDQTHSWTMPIVLLIAALIVMGAAGQGAGRDRYR",
    "response#2": "QSFWLITGIIFIAFNLRPAITSVGPVISSIRAELHMSNGAAGFLTALPLLSFAVLSPLAPKLGQRLGNERTLWLGLVILLIGVLTRSTGYTAALFFGTALIGVGIAIGNVLLPSLIKHKYPEKPGIMISLYTTSMNIFAALASGVSVPLATQMNGGWKQAFLLWGGLALLALLIWIPQLRHRDTANQTMKLQSSSIWASKMAWYVTIFMGLQSFLFYSSIAWFPEILRSHGIDTATAGWMVSLMQFASLPSTFLTPVLADRVKQQRGIVAALASVYLIGLCGLLAGGSHTLLAIWMIIIGIGQGSSISLALTLIGLRSENAQQAAALSGMSQSFGYLLAAVGPIFVGYLFDQTHSWTMPIVLLIAALIVMGAAGQGAGRDRYS",
    "response#3": "QSFWLITGIIFIAFNLRPAITSVGPVISSIRAELHMSNGAAGFLTALPLLSFAVLSPLAPKLGQRLGNERTLWLGLVILLIGVLTRSTGYTAALFFGTALIGVGIAIGNVLLPSLIKHKYPEKPGIMISLYTTSMNIFAALASGVSVPLATQMNGGWKQAFLLWGGLALLALLIWIPQLRHRDTANQTMKLQSSSIWASKMAWYVTIFMGLQSFLFYSSIAWFPEILRSHGIDTATAGWMVSLMQFASLPSTFLTPVLADRVKQQRGIVAALASVYLIGLCGLLAGGSHTLLAIWMIIIGIGQGSSISLALTLIGLRSENAQQAAALSGMSQSFGYLLAAVGPIFVGYLFDQTHSWTMPIVLLIAALIVMGAAGQGAGRDRYS"
  },
  {
    "reference": "MIARILYFFLWSAAFLPELQCASQRTADALTTFPTSAFINGTDRKDSNQTSTSRIKRKTLSVDFAVPSLLRYYLALFIKRPLNGDCLSFNGCYTVRANLLMRCVPLQKTIAGLLDAKLAAMNVNRSSTGQLPYRQKRPVPKVLNLGLTSASRKSNQVVVEVGEEMVKTGCGGLHVYEDAPVVFLEMDLTRILEWWLGAEGGRLRVRLMPERKVQVPGKEDKYSAAIRASDARLFIQIASSERPSSTISRNPTVAPKYWNFSWIAEDELTFPEDPVSTSDCTSKAKSCDRRPDGYYPEFAWSLTSAEDSWAIDQTMVKTKASSQGWEEGRFLTVNSSALTGPWVLSPWFRAAHRPCGLDITVFLHPRQSGRYTVWLIERDKPPLALLTTEHPHVIGWAVVHLSLAARSKPFRISSSYSQPGEIETATYDPRYSTNCTTRSSQNVTLRGRYHCRGGREINVSQLCDFSIDCPQGDDEGEHCRQFLNGSYCSFGREDCGWQPVQGRGPQWRAHPSIPQSLRSSCPSPGALLAIDSQPKGQRGSAQVRSPLFFYPLRNAPCMVKFWVCGSSNGALSLWITENSTGPEGQRSLWNSTSEANMGKGWKLITLPLFGLVDLFYLQFSADISSSSGIAFAVDNFTLSMECFLETNGEFPPVAPISPTQALFTQSNENIKTTTTLYGGPGASTESVKWIFHTCGATGQDGPTPTQCSNSYRNTNVNVTVGTKGPFKGIQMWQVPETRKYRITAYGAAGGRSVLAVHKSHGVYMTGDF",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be expressed specifically in the plasma membrane2. The Extracellular topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.3. The protein must exhibit the following characteristics: Ligand-binding to the extracellular domain induces tyrosine kinase activation, resulting in the activation of the mitogen-activated protein kinase (MAPK) pathway. The designed protein sequence is ",
    "response#1": "LQAAYVSKPGAALAHEMGLGLNDDILYGVFAQSKPDSSEPTNRSAVCAVSVRTINEFFNKIVDKQNMKCLQHFYGKDSKYCLNRAFSRNASYCRAQDDEYRLEVTTPLQRVDLFMGQFNNILLTSISVFTKGNLTIANLGTSEGRFMQIVVSRSESTAPHVSFQLDSHPVSC",
    "response#2": "LSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHGEVALYKNLLHSKESSCSDSTNEYMDMKPGVSYVVPTKADKRRSARIGSYIERDVTPAIMEDDEC",
    "response#3": "LSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHGEVALYKNLLHSKESSCSDSTNEYMDMKPGVSYVVPTKADKRRSARIGSYIERDVTPAIMEDDEA"
  },
  {
    "reference": "MDPFLVLLHSLSTRLSDRELTELKFLCRNRVSKRKLELMESGLDLFNVLLEQNDLDAERTDLLRQLLTSLRRQDLLQCLDSFEVEATAGASLRETDLRTAFDIICDNVGRDWKRLARQLNISDTKIDAIEERYPRNLPERVRESLRIWGNLQKENATVARLVQVLRDCRMNLVADLVEEEQQARESQNESKSMSSMAWESEASSSQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.; Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis; The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation; Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors2. The protein contains novel Death domains that confer a unique function or activity.3. The ideal protein would be one that plays a critical role in death-inducing signaling complex assembly through tumor necrosis factor receptor superfamily binding, caspase binding, death effector domain binding, identical protein binding within CD95 death-inducing signaling complex. The designed protein sequence is ",
    "response#1": "ETELLRDTFTSLGYEVQKFLHLSMHGISQILGQFACMPEHRDYDSFVCVLVSRGGSQSVYGVDQTHSGLPLHHIRRMFMGDSCPYLAGKPKMFFIGR",
    "response#2": "ETELLRDTFTSLGYEVQKFLHLSMHGISQILGQFACMPEHRDYDSFVCVLVSRGGSQSVYGVDQTHSGLPLHHIRRMFMGDSCPYLAGKPKMFFIP",
    "response#3": "RYKMKSKPLGICLIIDCIGNETELLRDTFTSLGYEVQKFLHLSMHGISQILGQFACMPEHRDYDSFVCVLVSRGGSQSVYGVDQTHSGLPLHHIRRMFMGDSCPYLAGKPKIFFIQNYVVSEGQLEDSSLLEVDGPAMKNVEFKAQKRGLCTVHREADFFWSLCTADVSLLEWSHSSPSLYLQCLSQKLRQERKRPLLDLHIELNGYMYDWNSRVSAKEKYYVWLQHTLRKKLIPSC"
  },
  {
    "reference": "MVERLAELERALGITFRDRKLLQTALMHRSLFNEQPHLLSNLSDNERLEFLGDSVLHFVTTTWLFQQFPHQNESTLTSWRAALVSTKGLAECAATLNLGQYAFLSRGEDTPKGRQRSKLLADLFEAVIGAIYLDQGLEAARAFIEPFLHERIGTLQTAVLDPTTRLQEIVQSRHKQLPKYYLIDERGPDHQREYVMAVTVEGHEYTGTGPSKKAAKKAAAQAALADIDR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.2. The protein contains novel RNase III, DRBM domains that confer a unique function or activity.3. The designed protein should be involved in mRNA processing, tRNA processing, rRNA processing by localizing to cytoplasm and exhibiting rRNA binding, metal ion binding, ribonuclease III activity.4. The protein must exhibit the following characteristics: Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S); Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon; Digests double-stranded RNA.5. The designed protein is the optimization of the active site to achieve high catalytic efficiency for Endonucleolytic cleavage to 5'-phosphomonoester.. The designed protein sequence is ",
    "response#1": "MDGVDELLLRKFNLTLPPGLLRTAFVHKSFSFENGACQNNERLEFLGDAVLGLVVSHYLFETCPEYTEGQLSAARSYIVSGTSIAQIAKELNLGQFMLLGKGEKLAGGMDKTSLLADLLESLIGAVMVDQFERARQRLDKEGSLLTTAFTHVSALKSSRGRCDSYQRLEFLGDHVLGLVVSDMLYRAFPKADEGELSKRLADLVRKETCIDVAKSLDLQEGVKLGAVGAGAGARLRRSVLGDICEAVIGAVYLDGGYAAAAAFVERNWLERMRKPVRERLSSVLKLEAIDYKTDLQELLQQNGPVNIEYSVISESQLPSHFVVELKINDQVQTQGEGHNKKAAEQQAAKAALQKFL",
    "response#2": "MDGVDELLLRKFNLTLPPGLLRTAFVHKSFSFENGACQNNERLEFLGDAVLGLVVSHYLFETCPEYTEGQLSAARSYIVSGTSIAQIAKELNLGQFMLLGKGEKLAGGMDKTSLLADLLESLIGAVMVDQFSRLRMSRPEDAKTELQEWAHQAASAVPAYQIDSREGPDHDPLFTVSVRVGAFQPATGSGRSKREAEQAAAAALG",
    "response#3": "MERTGHAFADRQRLQRALTHASARGANAGTDYERFEFLGDRVLGLVVADMLLAAFPDAAEGELSLRLNALVNAEALSEIAEHIGLPDLIRAGSDVRGLEGRKRVNLRADALESLIAVLYLDGGFRAVRDRLRREGLRYKSLLIEWCQKEKRVFHYDIYEDEDAGKLKYFGVKLSIDGKVVAKARATSKKKAEEIASKRGYFVFD"
  },
  {
    "reference": "MTLITVVVGIFALIYSIQSNSQAAAAGRSPAGSVAVSLPKLAAAVADGEVKGLVIRDNLLIATQKDGSQLTARKEANISALETLRVLGVPAKILTDLPVVIEAEQEEGISFGPLSFLSIFIFVALLGFFVFRGLRPSPSSNSPFNNGQMGRSNPRIFSGSSDRHGDAASKSLPPPVTFRHVAGVEQAKLELQEVVEFLKEPSKFVRLGARVPRGVLMAGPPGTGKTLLAKAVAGEAMVPFFSISGSEFVEMFVGVGAARVRDLFKRAREQAPAVIFVDEIDAVGRRRGANFGSGNDEREQTLNQILVEMDGFPTDVNVIVMASTNRPDILDPALLRPGRFDRKVILDQPDVNAREAILEIHTQGKPLAPNISIPDLARLTPGFVGADLENLVNEAAILAARRNLNSIGRLEFQDAMERIIAGPERQGKILSDYEREVVAYHEAGHALVMHHLAHSDPVHKVSIISRGMALGYTMPLPNGDKYLRSRPAFEDEITGLLGGRAAEEIIFQQITTGAANDLERATKLARAMVTRFGFSEKLGLRTFGEEQGNTYLGHLGELRDYSEGMAQLIDQEIHGILERAYERAKTVITSQHGKLVSLALALLERETVERQEFESLMA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have ATP binding, zinc ion binding within plasma membrane to regulate protein catabolic process.2. The protein must exhibit the following characteristics: Plays a role in the quality control of integral membrane proteins..3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.4. The ATP binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "LLVGPPGTGKTLLAKAVAGEADVPFFSLSGSDFVEMFVGVGAARVRDLFKQAKAKAPCIIFIDEIDAIGRSRSRGQVPGGNDERENTLNSLLVEMDGFATDSGVIILAATNRPDTLDAALLRPGRFDRQISILHFDVDVKILARGTPGFSGADLENLVNEAALLAAKRNKKKVEMEDFEGIL",
    "response#2": "IPKGVLLVGPPGTGKTLLARAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFLQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFEANEGVIMIAATNRPDVLDPALLRPGRFDRRVVVPRPLNFKPATLEEDVDVKILARGTPGFSGADLENLVNEAALLAAKRNKKKVEMEDFESALNYVVE",
    "response#3": "VLLNGPPGTGKTLLARAVAGEAGVPFFSVNGSEFIQMFVGVGASRVRDLFRTAKENSPSIIFIDEIDAVGRQRGAGLGGGNDEREQTLNQILGEMDGFSSNQAVIVVAATNRPDVLDPALLRPGRFDRHITVHFDVDVKILARGTPGFSGADLENLVNEAALLAAKRNKKKVEMEDFEQANQRLLEALDADGLEA"
  },
  {
    "reference": "MVEAAPPGPGPLRRTFLVPEIKSLDQYDFSRAKAAASLAWVLRAAFGGAEHVPSELWEPFYTDQYAQEHVKPPVTRLLLSAELYCRAWRQALPQLETPPSPAALLALLARRGTVPALPERPVQEADLRHQPILMGAHLAVIDALMVAFALEWTKTVPGPLALASLEHKLLFWVDTTIRRLQEKTEQEAAQRASPAAPADGVAPAQPSCPTRWYWKLVPHAIAFCLKESGSKPPMIRYRKDRAVARRAPCFPTVTSLQDLASGAALAATIHCYCPQLLRLEEVCLKDPMSVADSLYNLQLVQDFCACRLPRGCPLSLEDLLYVPPPLKINLVVLLAEMFMCFEVLKPDFVQAKDLPDSHAASPRAMEASTPQNGSGSSSPVFNFRHPLLSSGSPQSPLRGSTGSLKSSPSMSHMEALGKAWNRQLSRPLSQAVSFSTPFGLDSDVDVVMGDPVLLRSASSDSLGPPRSVPARTPAQPPPEPGDLPTIEEALQIIHSAEPRLLPDGAADGSFYLHSPEGPSKLPLASPYPPEGASKPLPDGPTKAPVYLPHPEGPLKPSPCPAGEVSKLPALSEGSPKAAASSPAASNSEVKMTSFAERKKQLVKAEPEAGSPAAGSAAPEALSSEMSELGARLEEKRRAIEAQKRRIEAIFAKHRQRLGKSAFLQVQPREAGGEVEVEEELGPASGGERPAGEGQGEPAPRPKAVTFSPELGPAPPEGLGDYNRAVSKLSAALSSLQRDMQRLTDQQQRLLAPPEAPAPAPPPAAWVIP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be involved in establishment of epithelial cell apical/basal polarity, cilium movement, regulation of cell migration, epithelial cell-cell adhesion by localizing to centrosome, zonula adherens, microtubule minus-end and exhibiting microtubule minus-end binding, spectrin binding, calmodulin binding.2. Prioritize Pro residues, Polar residues, Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.3. The presence of Calponin-homology (CH), CKK domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "SGNKPPVIRYRKDKALPKQTPCFPAIAGMKDLSSGGAVAATIHYYCPQVIRLEDVCLKETMSVADSLYNLQLIQGFSSDYLGGACFLSLEDLLYMPPALRINVGVFLAELFLCFK",
    "response#2": "LQRVGKRAAKFQFVACYHELVLECTKKWQPDKLVVVWTRRNRRICSKAHSWQPGIQNPYRGTVVWMVPENVDISVTLYRDPHVDQYETKEWTFIIENESKGQRKVLATVDVNLAHHAGPVPAQVPLRLRLKPKSVKVVHAELSLTLSGVK",
    "response#3": "SGNKPPVIRYRKDKALPKQTPCFPAIAGMKDLSSGGAVAATIHYYCPQVIRLEDVCLKETMSVADSLYNLQLIQGFSSDYLGGACFLSLEDLLYMPPALRINVGVFLAELFLCFS"
  },
  {
    "reference": "MERKLALLLILGMVTLASCGLREKHVQKLVALIPNDQLRSILKAVVHKVAKTQFGCPAYEGYCNDHCNDIERKDGECHGFKCKCAKD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should contain one or more BetaSPN-type CS-alpha/beta domains that are essential for its function.2. The designed protein must be able to perform potassium channel regulator activity under specific conditions. The designed protein sequence is ",
    "response#1": "KEGYPVGRDGCKISCVINNNFCKVECQAKWRQSDGYCYFWGLSCYCTNLPEDAQVWDSSTNKCR",
    "response#2": "KEGYPMGRDGCKISCVINNNFCKVECQAKWRQSDGYCYFWGLSCYCTNLPEDAQVWDSSTNKCS",
    "response#3": "KEGYPVGRDGCKISCVINNNFCKVECQAKWRQSDGYCYFWGLSCYCTNLPEDAQVWDSSTNKCK"
  },
  {
    "reference": "MNRIQSVFSQLKSQNRAALIPFITAGDPDATTTVALMHRLTQAGVDLIELGVPFSDPMADGPTIQRSSERALKHHISLKDVFSMVAEFRKTNQSTPVVLMGYANPIEAMGYKDFVQTAGHAGVDGVLVVDYPPEECTEWVRYLKEQNIDPIFLLSPTTPESRIRRVAELARGYVYYVSLKGVTGASHLDLHEVGDKLSQLRSYINIPIGVGFGIRDEQTARRIAEQADAVVIGSRIVEEIEHSPAADLLANVGALVESLRRAIDAKSDHSSITEK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate..2. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. The protein should be optimized for activity in Amino-acid biosynthesis, L-tryptophan biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MIPGL",
    "response#2": "MSGR",
    "response#3": "GRSDRRDD"
  },
  {
    "reference": "MPVNLEYLGFKLPGNVDEKYVQFKLKYRKQYHETEDEIRFNIFKSNILKAQLYQVFVRGSAIYGVTPYSDLTTDEFARTHLTASWVVPSSRSNTPTSLGKEVNNIPKNFDWREKGAVTEVKNQGMCGSCWAFSTTGNVESQWFRKTGKLLSLSEQQLVDCDGLDDGCNGGLPSNAYESIIKMGGLMLEDNYPYDAKNEKCHLKTDGVAVYINSSVNLTQDETELAAWLYHNSTISVGMNALLLQFYQHGISHPWWIFCSKYLLDHAVLLVGYGVSEKNEPFWIVKNSWGVEWGENGYFRMYRGDGSCGINTVATSAMIY",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: May be crucial for metabolism of host hemoglobin..2. The protein must contain a signal peptide for proper functionality.3. A protein that can perform cysteine-type endopeptidase activity in lysosome to support proteolysis.4. The active site of the protein must be engineered to enable effective catalysis of Specificity close to that of papain. As compared to cathepsin   B, cathepsin L exhibits higher activity toward protein substrates,   but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-   dipeptidase activity.. The designed protein sequence is ",
    "response#1": "EGVVTGGNYRKQGCCRPYEFPPCGRHGKEPYYGECYDTAKTPKCQKTCQRGYLKAYKEDKHFGKSAYRLPNNVKAIQRDIMKNGPVVAGFIVYEDFAHYKSGIYKHTAGRMTGGHAVKIIGWGKEKGTPYWLIANSWHDDWGEKGFYRMIRGINNCRIEEMVFAGG",
    "response#2": "EGVVTGGNYRKQGCCRPYEFPPCGRHGKEPYYGECYDTAKTPKCQKTCQRGYLKAYKEDKHFGKSAYRLPNNVKAIQRDIMKNGPVVAGFIVYEDFAHYKSGIYKHTAGRMTGGHAVKIIGWGKEKGTPYWLIANSWHDDWGEKGFYRMIRGINNCRIEEMVFAGR",
    "response#3": "VPDEVDWRDTHLVTDVKNQGMCGSCWAFSATGALEGQHARKLGQLVSLSEQNLVDCSTKYGNHGCNGGLMDQAFEYIRDNHGVDTEESYPYKGRDMKCHFNKKTVGADDKGYVDTPEGDEEQLKIAVATQGPISIAIDAGHRSFQLYKKGVYYDEECSSEELDHGVLLVGYGTDPEHGDYWIVKNSWGAGWGEKGYIRIARNRNNHCGVATKASYC"
  },
  {
    "reference": "MSEFDKKLNTLGVDRISVSPYKKWSRGYMEPGNIGNGYVSGLKVDAGVVDKTDDMVLDGIGSYDRAETKNAYIGQINMTTASSFSGVGGTVISYDILRNPEVDKAKPLFTEKQWD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with histidine decarboxylase activity functions is required to enable histidine metabolic process.2. The protein must bind to pyruvate in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "GTQGVLQGTYETLSALARKEFKKDDLSGKWVLTSGLGEMGGAQPLAITMNNATGIIVEVDEEKIRRRLRDKYLDTWTDSLDEALRLKDEALKSGKPTSIGLLGNAATVYDELMKKNIVPDVVSDQTAAHDLNLGYIPEGYTVQSAAEFREKDKDGYVKKVYASIVKEAEAILWFMQHGSKAFDYGNNFRTRALEGGLKNAFDIPGYVPAYITLSKAFSLANEQKVFLRRIEGILHAQNYLMLDYNIDRVNLAASEKITPGISGPTVSPLARENWVAVRAMVPRKEANHIMDQLSELGAQAILASEIL",
    "response#2": "GTQGVLQGTYETLSALARKEFKKDDLSGKWVLTSGLGEMGGAQPLAITMNNATGIIVEVDEEKIRRRLRDKYLDTWTDSLDEALRLKDEALKSGKPTSIGLLGNAATVYDELMKKNIVPDVVSDQTAAHDLNLGYIPEGYTVQSAAEFREKDKDGYVKKVYASIVKEAEAILWFMQHGSKAFDYGNNFRTRALEGGLKNAFDIPGYVPAY",
    "response#3": "GSQGIVQGTYETFAEVARQHYGGTLKGTITVTAGLGGMGGAQPLAVTLNGGVCIAVEVDPARIQRRIDTKYLDTMTDRLDVAIQMAKRAKEEGKALSIGLLGNAAEVLPKMIEIGFIPDVLTDQTSAHDPLNGYIPAGMTLEEAAELRQRDPKQYIRRAKQSIAEHVKAMLAMQKQGSVTFDYGNNIRQVAKDEGVEEAFNFPGFVPAYVEPY"
  },
  {
    "reference": "MTQEHLTNNINQKSPIRVGLLGFGGLGQAAARILAAKREMILVAIADKHGYAYNPEGLNPDQCIAAYHQQGSVGYLEAGGILSHNSIEELISSAQVDGYFLALPNLPNTFMASVAQQFIASGWRGVLVDAMKRTSAVEQLLALKADLNAAGITYMTGCGATPGLLTAAAALAAQSYTEIHSVKITFGVGIANWEAYRATIREDIAHIPGYTVDRAMAMTDEEVAALLDRTEGKITLENMEHADDIMLELAGICPRDRVTVGGVVDTRNAKKPLSTNVQITGRTFEGKISTHTFTLGDETSMAANVCGPAFGYLKAGINLQQRGIYGLFTAAEIMPQFVK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Catalyzes the formation of (S)-8-amino-7-oxononanoate (DAN-carbamic acid) from (7R,8S)-8-amino-7-(carboxyamino)nonanoate (DAN), a function equivalent to the cannonical BioA reaction and the first half-reaction of BioD2. The Proton acceptor, Proton donor and proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.3. The NAD(+) binding site should be stable and able to withstand changes in environmental conditions.4. A protein should be able to manipulate the activity of enzymes in the biotin biosynthesis pathway, either by activating or inhibiting their function.5. The designed protein should have NAD binding to facilitate amino acid metabolic process, biotin biosynthetic process. The designed protein sequence is ",
    "response#1": "MFRVGVLGFGGLGQAAAKILAAKQEMTLVAAADHKGYVYRSAGLDIDAAISAYDKHHSVGYLATGGVLA",
    "response#2": "MPRVGVLGFGGLGQAAARVLAPKSEMILVAAADKKGYVYNPEGLDPNTCIAAYNSQGSVGHVENGGVLSNDSIAADQAKRIGVLGFGGLGQAAAKVLAPKKEMILVTVADKKGYAYSPEGLNAKECIATYHSQGSVGYLDIFGTLTNYSIQDLIESGHPVDGYFLALPNLPNDFIASVAQQFIKSDPEYIRVGVLGFGGLGQAAARVLALKQEMQWVAAADHKGYVFAPSGLSVEDCVKTYQTQGSLGYLD",
    "response#3": "MSKVGILGFGGLGQAALKLLSTKQEMLCVALADQKGYVYASQGIDVNKAIATYQAKGSVGYLESGVLTKVGILGFGGLGQAALKLLSTKQEMLCVALADQKGYVYASQGIDVNKAIATYQAKGSVGYLESGVLTKVGILGFGGLGQAALKLLSTKQEMLCVALADQKGYVYASQGIDVNKAIATYQAKGSVGYLESGVLT"
  },
  {
    "reference": "MKKNINIFNISTSKINLLDLNRQDLRCFLISLGAKSFCAEQIMYWIYHDYCNDFKKMFNISIKIRNQLYEKSYIFAAKFIEERVSCDGTIKWITSINNQKIETVYIPEKKRSTLCISSQIGCPLQCHFCATGKEGFQRNLKVSEIIAQIWQANKMLKEKNITKNITNIVFMGMGEPLLNLKNVVSALKIILDEYGFGLSKRRVTLSTSGIVPALDKLNSMIDVSLAISLHAPNNYIRNMIMPINRKYNIDSVLKSAFKYFKSSNANRGGVTIEYVMLDGINDSNKNAQELAFLLRKIPSKINLIPWNSFVGSSFLCSDINRINIFANILRKKGFTAMIRKNRGQDIHAACGQLTSGMKNSLKKKFV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining rRNA binding, tRNA binding.2. The designed protein should have a high specificity for [4Fe-4S] cluster binding, with minimal off-target effects.3. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "SQSGCPLKCDFCLTAKLGLLRNLTAGEIVEQIIIVLNDVYGVGTATPHGTNLVGMGAGEPFMNFENLIKALGIMSSEEGLFIVPNRVTVSTAGIVPKIYEIAQLGNRPHLAISLSAPTDELRTKLMPINKRWNIDEVMKAARVFEKSLKRGERFTFEYVLLGGVNDSDEQAVELTLEKFALKRPPRHLADLTPDERAVVAKEAGLPGFRAKQLSRHYFARFVDDPAEMTDLPVELRSQLQAALLPVLATAVHTQEADGGATRKTLWKLHDGALVESVLMRYPGRVTMCVSSQAGCGMACPFCATGQAGLQRNMSTGEIVEQVVAGARLLARNQVPGGPGRVSNVVFMGMGEPMANYNAVMGAVRRMVDPTPDGLGMSARGITVSTVGLVPRMLQLADEHIPVTLALSLHAPDDDLRNELVPINTRWSVREAVEAAWNYARVTRRRVSIEYAMIKDINDQGWRADMLADVLRSYGDWGWVHVNLIPLNPTPGSKWTASRPEDEREFVRRLIDKGVPTTVRDTRGREIDGACGQLAR",
    "response#2": "EDDRGTLCISSQAGCAVNCRFCSTGKQGFSRNLNLGEIIGQLWLAEHSLRRDPQAIRRIDSYDGTPIEEHEGRVISNVVLMGMGEPLLNYEPVVGAMRLMLDDHAYGLSRRRVTLSTSGVVPMMDRLANDLPVALAVSLHAPNDALRDELVPINKKYPLKELMAACMRYLPFAPRDFLTFEYCMLDGVNDTDQHAKELVDLLKKIPCKLNLIPFNPFPESGLKRSSNARVQDFAKRLMDAGIITTVRKTRGDRLQAACGQLAGQYERRRTACISTQVGCAMGCAFCATGQMGFRRNLTSGEIVAQALHFARHLRRDGERLTNVVLMGMGEPLANYDASLAAIRRLIHPQGFRLGRRHVTVSTVGLVPGIRRLAEENLPITLAVSLHAATDALRDRLVPVNRRYGLDELFAACQDYVERTGRRVSFEWALIAEVNDTAEQAQALCARLVGLKAHVNLIPLNPTPGYAGRPSPDERIAAFTAELRRCRVPFTLRLRRGGEAADACGQLAAEG",
    "response#3": "SQSGCPLKCDFCLTAKLGLLRNLTAGEIVEQIIIVLNDVYGVGTATPHGTNLVGMGAGEPFMNFENLIKALGIMSSEEGLFIVPNRVTVSTAGIVPKIYEIAQLGNRPHLAISLSAPTDELRTKLMPINKRWNIDEVMKAARVFEKSLKRGERFTFEYVLLGGVNDSDEQAVEFVEK"
  },
  {
    "reference": "MNNSNSDFPAGNSSGTTKHIFVTGGVVSSLGKGLTAASLGNLLTARGLRVVMQKLDPYLNVDPGTMNPFQHGEVFVTDDGAETDLDIGHYERFLDINLSQAANVTTGQIYSQVIAKERRGEYLGDTVQVIPHITDEIKRRMRLQATDDPKPDVIITEVGGTVGDIESQPFIESARQVRHELGRGNVFFVHVSLVPFMNASGEQKTKPTQHSVAALRSLGIQPDALVLRSDRPVSESNKRKIALMCDVEEDAVVNCVDVPSIYDIPTMLHDQGLDAYIIDALDLPTSSVDWTSWQKVLDAVHEPKQEVNIGLVGKYIDLPDAYLSVTEALRAGGFAHQAKVKLHWVASDECETEEGAARLLSDFDGICIPGGFGVRGIEGKVGALRFARENGIPVLGLCLGLQCMVIEYARNKAGLPGASSTEFDPDTEFPVIATMEEQVEIIAGGDLGGTMRLGLYTANLAPGSLVADLYGSPQASERHRHRYEVNNAYREQIADAGLWFSGTSFDRQLVEYVELPRDEHPFYVGTQAHPELSSRPNRANPLFRGLIGAAIERQRASRLFEVKEEA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein with the reaction H2O + L-glutamine = L-glutamate + NH4(+), the active site, Nucleophile, Nucleophile; for glutamine hydrolysis must be optimized to ensure efficient catalysis.2. A protein that can modulate the overall flux of metabolites through the CTP from UDP: step 2/2, Pyrimidine metabolism, CTP biosynthesis via de novo pathway pathway, enhancing or suppressing its activity as needed.3. The protein contains novel Glutamine amidotransferase, CTP synthase N-terminal domains that confer a unique function or activity.4. The designed protein should have a high specificity for CTP, L-glutamine, ATP, UTP, Mg(2+) binding, with minimal off-target effects.5. A protein with CTP synthase activity, metal ion binding, glutaminase activity, ATP binding functions is required to enable glutamine metabolic process, 'de novo' CTP biosynthetic process.6. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. The designed protein sequence is ",
    "response#1": "MFITGGVVSSLGKGIAAASLAAVLEARGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFVCMTATRRNNFTTGRIYENVIRKERRGDYLGGTVQVIPHITDEIKRCIRLGAGEADIAMIEIGGTVGDIESLPFLEAIRQMGVELGRERSLFIHLTLVPFIKSSGELKTKPTQHSVKELRSIGIQPDILMCRSDREIPAEERRKIALFTNVEPKAVINVPDADTIYRIPLLLRAQGVDDIVARKLQDSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASFVAAASYVALQDAYLSVIESLKIAGWEFNKKVKIKWIQADKLNEQNYKEVLKESQAILVPGGFGNRGIEGMMLASRFARENDIPYLGICLGMQIATISMARDLLGWKDANSTEFDETTSHAIFDYIRGIDRHNIGGTLRLGTMATKLEKDSIAQKLYNSDNAYERHRHRYEFNNEYKKDLESVGLRFSGIYEEQNLVEIVEIPKLKFFVASQFHPEFTSRPNKPSPLFKGFIAAAQAKLQ",
    "response#2": "MFVTGGVCSSLGKGIATASIGAIMKAAGLKVFVQKLDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDEPMSRLSTVTTGRIYSDVIAKERRGDFLGGTIQVVPHITNAIKHSIISAAEQSGTEIMMVEIGGTVGDIEGEPFLEAVRQMRGELGPQRIFHVHLTLLPYLKGSKELKTKPTQLSVRELRRIGLQPDMILARADYRVPKDLLEKISRFCGVPSEAVIPALTVNSIYDVPLNFQKYDVARIIGEKLGLVAPPEPDLSKWQDFLTRLKAAKHTINIGLIGKYVELHDAYKSISEAFIHAGATNEVKVKIHWIHSEELKAADVAGLLKGLNGILVAPGFGERGIEGKIKAVQYARENNIPFFGICLGMQMAVIEFSRNVLKLADANSIEMDENTSNPVINLMEEQKEITNMGGTMRLGAWDCKLTENSKLKDIYNSEIISERHRHRYELNNKYVADLETAGMTVSGKNPVTDLVEVIEIPSHPWFIGVQYHPEYKSTVENPHPLFVAFVKAALAHQA",
    "response#3": "MFITGGVVSSLGKGIAAASLAAVLEARGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFVCMTATRRNNFTTGRIYENVIRKERRGDYLGGTVQVIPHITDEIKRCIRLGAGEADIAMIEIGGTVGDIESLPFLEAIRQMGVELGRERSLFIHLTLVPFIKSSGELKTKPTQHSVKELRSIGIQPDILMCRSDREIPAEERRKIALFTNVEPKAVINVPDADTIYRIPLLLRAQGVDDIVARKLQLTDAYKSLNEALLHAGIHAHTRVNIHYIDSERVERDGTACLEHMDAILVPGGFGERGIEGKIAAVRFAREHRLPYLGICLGMQVAVIEFARHVAGLEGAHSTEFRKDTPHPVIALITEWIDEDGSVQQRREGGDLGGSMRLGAQPCRLVADSLAGATYGKDVIVERHRHRYEFNNRYRGPLGAVGMRFCGHSLDGRLVEMIELPDHPWFLACQFHPEFTSTPRAGHPLFRGFLNAAAGRISVKIAVVGKYVELRDAYMSVKEALLHAAAANGRHLEILWLNSEDLEKGKGWDVLSQAQGIVVPGGFGYRGIEGKIMAGRFARENKVPYLGLCLGMQTMCIEFARNVLGLEDANSSEFEVTSENYVIDMMLEQRGITDLGGTMRLGVYPCDLQAGSRAAEAYGTAKVDERHRHRYEFNNEYRGSFEAHGMRFSGLSPDGQLVEISEVVDHPFMVGSQFHPEFLSRPNRPHPLFNALLRAAIAART"
  },
  {
    "reference": "MSTALIILAAGKGTRMNSDLPKVLHPIAQAPMLEHAMAAGQALAPERTIVVAGHGADLVRAAVCDIDEAAQVVLQEEQLGTGHAVLQAKEALAGFEGDAVVLYGDTPFVSSETIERMVSARGRADLVILGFEAADPARYGRLVMQGDSLEKIVEYKDATDAERAITFCNSGLMACDAAQMFALLDKVGNNNASGEFYLTDLVEIARGEGLAVTAVSCDEAETLGINSRADLAQADAVFQAKARAELLDTGITLMAPETVYLSLDTVIGRDTVVEPNVVFGPGVTVESGALIRAFSHLEGCHVSRGAKVGPYARLRPGAELAENTHIGNFVEIKNAEIAEGAKVNHLSYIGDATVGKKTNIGAGTITCNYDGFMKHRTEIGSGAFIGSNTMLVAPVSVGDEAMTATGAVITKDVAAGDLALARTPQVNKPGRARKLMDLLRAKKERQ",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain..2. The designed protein have MobA-like NTP transferase domains with specific sequence motifs that are crucial for its activity.3. A protein with glucosamine-1-phosphate N-acetyltransferase activity, magnesium ion binding functions is required to enable cell morphogenesis, UDP-N-acetylglucosamine biosynthetic process, lipid A biosynthetic process.4. The designed protein should interact with enzymes and substrates within the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway to enhance or regulate its activity.5. The designed protein should have a high specificity for Mg(2+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MTQAVVMAAGLGTRMKSKRVKVLHPVAGRPMVLYAVELAMKLAAHRIVVVVGHQAEDVRRIVGEAVKGRAGGDAVVLVEQTRQLGTGHAVLQSRSAFVNGGKGLSEFLILNGDTPLLTEQTVRELLRVHHEEKATVTMLTAQVDDPTGYGRVVRRESGDKRTPLSTGDVLKIVEDRDATPAERAIKEINVGTYVVSADFLFPALDQLEPRNAQGEYYLTDIVAAAVAQRRSVAAAYVTDDAAETQGIRDRNQLAAAERVLRQRANAELMLASTMRAEALENGVTLTAPETVFFAHDTIVGRETIIEPNVVFGPDVTVESGAVIRAFSHLEGCHVSRGAIIGPYARLRPGAEIGEEAHIGNFVEVKNAQIHEGAKANHLTYIGDATVGKGTNIGAGTITCNYDGFFKHHTEIGERAFIGSSTMLVAPVRVGNGAMTGSGSVITKDVEDGALALGRAAGVTMVDPDSVRIDADVLIGQDTVLHPNVMIEGKTEIGEDCIVRSHVRLTDCHVGNRVEILDHCVLRESLIEDDVVIGPFAHLRPGALVRRKAKVGNFVEMKKTELGEEAKANHLSYLGDAKIGRGVNIGAGTITCNYDGVHKYQTTIGDHVFIGSDTQLVAPVSVGDGAVIAAGTTVTKDVPPDALAIARTPQRNIEGWVARKRPGSAAAQAAAAALEAGEDHD",
    "response#2": "VIMAAGKGTRMKSSLPKVLHKLAGRSLLQHVLDTAATLGADRIITITGHGAELVEAAVQAPHLQFVRQMPQLGTGHAVQQAVPALHAEGITLILNGDVPLIRAETARALVEACDGTGRLGILTATVDDPTGYGRIVRDDAGRVRAVVEERDADPAERAEREINAGMYVVPAGWLWPALARVSTANAQQEYYLTDVVGLAVADGLPVGAHVTGDVLLGAGVNDRAQLAQLAALVRDRIVRRWQLDAGVRLADPARFDVRGTLTCGTDVEVDVNCVFEGDVSLGDGVRIGANCVIRNARIAANAVIHPFTHIDGEALGVKVGTGSLIGPFARLRPGADLGNDVHIGNFVEVKNSTLANGAKANHLAYLGDATVGERVNYGAGSITANYDGANKHRTVIEADVHVGSNSVLVAPVKLGAGATIGGGSTITKDVPAGALAVARGKQRNIEGWVQRKRPGTPAAEAAERARGKGASDAAGPDPTGGAA",
    "response#3": "MAVVMAAGLGTRMKSKRVKVLHPVAGRPMVLYAVELAMKLAAHRIVVVVGHQAEDVRRIVGEAVKGRAGGDAVVLVEQTRQLGTGHAVLQSRSAFVNGGKGLSEFLILNGDTPLLTEQTVRELLRVHHEEKATVTMLTAQVDDPTGYGRVVRRESGDKRTPLSTGDVLKIVEDRDATPAERAIKEINVGTYVVSADFLFPALDQLEPRNAQGEYYLTDIVAAAVAQRRSVVAYETEDMSESLGVNDRVALSDAERIMRQRINRLHMRNGVTMVDPDSVRIDADVLIGQDTVLHPNVMIEGKTEIGEDCIVRSHVRLTDCHVGNRVEILDHCVLRESLIEDDVVIGPFAHLRPGALVRRKAKVGNFVEMKKTELGEEAKANHLSYLGDAKIGRGVNIGAGTITCNYDGVHKYQTTIGDHVFIGSDTQLVAPVSVGDGAVIAAGTTVTKDVPPDALAIARTPQVVKEGWGVRLADPARFDVRGTLTCGTDVEVDVNCVFEGDVSLGDGVRIGANCVIRNARIAANAVIHPFTHIDGEALGVKVGTGSLIGPFARLRPGADLGNDVHIGNFVEVKNSTLANGAKANHLAYLGDATVGERVNYGAGSITANYDGANKHRTVIEADVHVGSNSVLVAPVKLGAGATIGGGSTITKDVPAGALAVARGKKINKEGWVEKKGLKK"
  },
  {
    "reference": "MAPSADPGMVRMALLLLPPLWLLPLTGGSQRAEPMFTAVTNSVLPPDYDSNPTQLNYGVAVTDVDHDGDFEIVVAGYTGPNLVLKYNRAQNRLVNIAVDERSSPYYALRDRQGNAIGVTACDIDGDGREEIYFLNTNNAFSGVATYTDKLFKFRNNRWEDILSDDVNVARGVASLFAGRSVACVDRTGSGRYSIYIANYAYGDVGPDALIEMDPEASDLSRGILALRDVAAEAGVSKYTAGRGVSVGPILSSSASDIFCDNENGPNFLFHNQGNGTFVDTAASAGVDDPHQHGRGVALADFNRDGKVDIVYGNWNGPHRLYLQMSAHGKVRFRDIASPKFSTPSPVRTVIAADFDNDQELEVFFNNIAYRSSSANRLFRVIRREHGDPLIEELNPGDALEPEGRGTGGVVTDFDGDGMLDLILSHGESMAQPLSVFRGNQGFSNNWLRVVPRTRFGAFARGAKVVLYTKKSGAHLRIIDGGSGYLCEMEPVAHFGLGRDEASSVEVTWPDGKMVSRSVASEEMNSVLEILYPQDEDKLQNTAPLECGQGFSQQDNGHCMDTNECIQFPFVCPRDKPVCVNTYGSYRCRTNKRCNRGYEPNEDGTACVAQVAFLGGYSSAAFRLSEPLSQASYLSLGLGLCLQLYAL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Ensure the presence of a signal peptide within the protein.2. The designed protein should be involved in negative regulation of receptor binding, axonal fasciculation by localizing to growth cone and exhibiting calcium ion binding. The designed protein sequence is ",
    "response#1": "PTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPI",
    "response#2": "GMLSGLLPDSQISASSMRDIHGATGAARLVASRSGWFPSPTQPVAGEEWLQVDLGVPKTVRGIITQGARGVDGSTSAENRAFVRKYKLAHSLNGKDLSYIIDPKTNLPKIFEGNTHYDTPEIRRFDEIVAQFIRVYPERWSPAGIGMRMEILN",
    "response#3": "GMLSGLLPDSQISASSMRDIHGATGAARLVASRSGWFPSPTQPVAGEEWLQVDLGVPKTVRGIITQGARGVDGSTSAENRAFVRKYKLAHSLNGKDLSYIIDPKTNLPKIFEGNTHYDTPEIRRFDEIVAQFIRVYPERWSPAGIGMRMEILE"
  },
  {
    "reference": "MKGSLLLAGATLLGCTSAKLHSLKLKKVSLKEQLEHADIDVQIKSLGQKYMGIRPEQHEQQMFKEQTPIEAESGHNVLIDNFLNAQYFSEISIGTPPQTFKVVLDTGSSNLWVPGKDCSSIACFLHSTYDSSASSTYSKNGTKFAIRYGSGSLEGFVSQDSVKIGDMTIKNQLFAEATSEPGLAFAFGRFDGIMGMGFSSISVNGITPPFYNMIDQGLIDEPVFSFYLGDTNKEGDQSVVTFGGSDTKHFTGDMTTIPLRRKAYWEVDFDAISLGEDTAALENTGIILDTGTSLIALPTTLAEMINTQIGATKSWNGQYTLDCAKRDSLPDVTFTVSGHNFTIGPHDYTLEVSGTCISSFMGMDFPEPVGPLAILGDSFLRRYYSVYDLGKGTVGLAKAK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein can be targeted to vacuolar lumen and perform aspartic-type endopeptidase activity. The designed protein sequence is ",
    "response#1": "YFSEISIGTPPQTFKVVLDTGSSNLWVPGKDCSSIACFLHSTYDSSASSTYSKNGTKFAIRYGSGSLEGFVSQDSVKIGDMTIKNQLFAEATSEPGLAFAFGRFDGIMGMGFSSISVNGITPPFYNMIDQGLIDEPVFSFYLGDTNKEGDQSVVTFGGSDTKHFTGDMTTIPLRRKAYWEVDFDAISLGEDTAALENTGIILDTGTSLIALPTTLAEMINTQIGATKSWNGQYTLDCAKRDSLPDVTFTVSGHNFTIGPHDYTLEVSGTCISSFMGMDFPEPVGPLAILGDSFLRRYYSVYDLGKGTVGLI",
    "response#2": "LPTEFDWREQGAVTPVKNQGMCGSCWSFSAIGALEGAHFLATKELVSLSEQQLVDCDHECDPAQANSCDSGCSGGLMNNAFEYALKAGGLMKEEDYPYTGRDHTACKFDKSKIVASVSNFSVVSSDEDQIAANLVQHGPLAIAINAMWMQTYIGGVSCPYVCSKSQDHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGYYKICRGPHNMCGMDTMVSTVR",
    "response#3": "QYFSEISIGTPPQTFKVVLDTGSSNLWVPGKDCSSIACFLHSTYDSSASSTYSKNGTKFAIRYGSGSLEGFVSQDSVKIGDMTIKNQLFAEATSEPGLAFAFGRFDGIMGMGFSSISVNGITPPFYNMIDQGLIDEPVFSFYLGDTNKEGDQSVVTFGGSDTKHFTGDMTTIPLRRKAYWEVDFDAISLGEDTAALENTGIILDTGTSLIALPTTLAEMINTQIGATKSWNGQYTLDCAKRDSLPDVTFTVSGHNFTIGPHDYTLEVSGTCISSFMGMDFPEPVGPLAILGDSFLRRYYSVYDLGKGTVGLAKC"
  },
  {
    "reference": "MIERFARLRAVVVGDVMLDSWLQGPATRMAQEAPVPVVRVERTEESPGGAANTAVNLAALGATVHLLGVIGDDRPGRLLARLLRRHGVTGLLTGERRTAVKRRLLAGGQLVARYDEEDRAPIGDHLIRRLIARLRRLAPRADVVIACDYGAGVCTDAVRHALRGLPLLVVDAHDVRPWAECRPDAVLPNYAEVCRMLGEPGDGGDRVAHLTAASRRILDAARARMVVTTLDGEGTLLHRPGRPPYRTHAVPAPDHMSTGAGDTFTAAFALALAAGASPEQAADIGQAAARVVVRRPGTVACTRHELVQELGTGGRAVPPEVLARHIAEHRRRGDRIVFTNGCFDVLHRGHVVCLEEARALGDVLVVAVNGDASVTRLKGPGRPINACEDRVAVLAALDCVDYVVVFEEDDPGELIRLVRPDVYVKGGDYHPELLPEAALVRELGGEVRVLDYVPDRSTTAIVNRIRTQA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein have Cytidyltransferase-like domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "VFKQIIDGLIDCEKVFENENFIAIKDRFPQAPVHLLIIPKKPIPRFQDIPGDEMILMAEAGKIVQELAAEFGIADGYRVVINNGAEGGQAVFHLHIHLLGGRPLGAIL",
    "response#2": "MTNGCFDILHAGHVSYLNNAAQLGERLIVAVNSDESVKRLKGSGRPVNTLDRRMAVLAGLEAVDWVVPFSEDTPQRVIAEILPSLLVKGGDYKPEEIAGGKEVIADGGAVKVLNFEDGCSTTKIIF",
    "response#3": "LTNGCFDVIHAGHVAYLRDAKNQGDVLVVGINVDEQVHAMKGEGRPVYALEDRMAILSELACVDYVIAFSEPTSHRLIESVMPDLFVKGGDYSPS"
  },
  {
    "reference": "MRVKGTQMNWPNLWKWGTLIIGLVMMCSASNDLWVTVYYGVPVWRDADTTLFCASDAKAHETEVHNVWATYACVPTDPNPQEIHLDNVTENFNMWKNNMAEQMQEDAISLWDQSLKPCVKLTPLCVTLNCTTVNLNSTNVNITKTPGLENITDEIRNCSFNMTTVLADKKQKVYALFYRLDIVPIDDNNSSNNSSDNQGREYRLINCNTSVIKQACPKISFDPIPIHYCTPAGYAILKCKDKTFNGTGPCNNVSSVQCTHGIKPVVSTQLLLNGSLAEGDIIIRSENLTNNAKTIIVHLNESVEINCTRPSNNTRTSIGMGPGQTFFRTGDIIGDIRQAYCQINGTKWNKTLGQVVKKLREHFNKTIKFQPPSGGDLEITMHHFNCRGEFFYCNTTKLFNSTHNFTTTEPVNNTGDDGTIILPCRIKQIINMWQGAGQAMYAPPISGIINCVSNITGILLTRDGGNTTDEAETFRPGGGNIKDNWRNELYKYKVVQIEPLGVAPTRAKRRVVDRKKRAVGLGAMIFGFLGAAGSTMGAASITLTVHARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLSDQKFLGLWGCSGKIICTTAVPWNSSWSNKSYEEIWHNMTWIEWEREISNYTQQIYSILTESQNQQDKNEKDLLELDKWANLWNWFSITKWLWYIKIFIMIVGGLIGLRIIFAVFSIVNRVRQGYSPLSFQIPSHHQRETDRPGRIEEEGGEQDRDRSVRLVSGFLALFWDDLRN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Incorporate a signal peptide in the protein design.2. The presence of Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains is critical for the interaction of the designed protein with its intended targets.3. The protein should have a specific function in positive regulation of receptor clustering, actin filament reorganization and should be localized to virion membrane in order to carry out this function efficiently.4. The protein must exhibit the following characteristics: Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state.5. The protein's functional design necessitates the inclusion of a YXXL motif; contains endocytosis signal motif. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWKDADTTLFCASDAKAHETEVHNVWATHACVPTDPNPQEIPLENVTENFNMWKNNMAEQMQEDVISLWDQSLQPCVKLTPLCVTLNCTNANLNATKCAAPLNNTDCASITGEIKDEVKNCTFNMTTELRDKEQKVHALFYRLDIVPIGSNDGNYSEYRLINCNTSVIKQACPKVSFDPIPIHYCTPAGYVLLKCNDKKFNGTGPCKNVSSVQCTHGIKPVVSTHLLLWQSGREEIIIKSENLTDNAKTIIVHLNESVAINCIRPFNNTRTSISIGPGQVFYKTGDIIGDLRKAFCEVNGKKWNEALKQVTVKLKELFNNKTIEFKFNHGGDLEIVTHHFVCGGEFFYCNTTQLFNHTGNNGTILLPCKIKQIINMWQRAGRAMCAPPISGKSRCASNITGILLTRDGGKHNGSEDKEVFRPEGGNIKDNWRNELYKYKVVQIEPIGIAPTKAKRRVVERAKR",
    "response#2": "LWVTVYYGVPVWEDADTTLFCASDAKSYSTESHNVWATHACVPTDPNPQEISLENVTEYFNMWKNNMVEQMHADIISLWDESLKPCVKLTPLCVTLNCTAKVSANMTDIY",
    "response#3": "LMKNCSFNTTTEIRDKQKQEYALFYIPDIVQMDDNSNSSNSSYSSYRMINCNVSTTKQACPKVSFDPIPIHYCAPAGFAILKCREKEFNGTGLCTNVSTVQCTHGIKPVVSTQLLLNGSLAEGDVMIRSENISDNVKNIIVQLNETIQINCSRPGNNTRKGIRIGPGQMFYATGEIMGDIRQAHCNVTRVAWKNMLGNVRKKLEEVTNRTIFFNSSSGGDLEVTSHIFNCRGEFFYCNTSNLFDTSLLSNSSEENNNGKNETITIPCKIKQIVRMWQRVGQAMYAPPIRGNITCESNITGLILTRDGGGNNSSDPEVFRPGGGDMRDNWRSELYKYKVVKIKPLGIAPTRARRRVVEREKR"
  },
  {
    "reference": "MRSDAIKRRLYSKAIFIIPFWILSIALWFSSGSVYKIDSCMIDNNKTTCIPYTISKYTDFNSNSQLNQFNSSFILFQIIPINILCFLPLLGYMYILASVLKVDLVKKAFTIFGIMMLITIPLFLIVSICIFPLNLRETLCSDLKNGFIKSNIVDKGNTTICNLKESFNYFKGSFSDTTTTTTTTTTLEWGITYNWYFIIATIVFSFSMLVIVLVLLIKDANTLNNGDGQNYSLFEQENDENFEDDDPNDVIESGLDLWGRNIWYNYTKKPMNSILLSIFQLFYIGSYFSLYYFGFKFNLIPFFINLIILFISYYNLIFDGIRDNYLIKRMYRERSNDVNQFIERVKKIKPKVAIVKKPNKEGGKSSSGGGGGGGGGIDDGIEAILEPRLLKSWLDQSELDYLMINDDDDKYKPIVELTIIEDFKTTSQLPIDAISLLFVKTDLSNYYIITNKKKRFIQINRGLHSFLVLIGLSGLFNILYLKRLILKKSITIKTLFEYNNR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein localizes to the membrane. The designed protein sequence is ",
    "response#1": "SVHKLRPGDIDVIAAMGDSLTAGAGIFADNEFNPNLIGFALGDSLTTHKESQLNIAESGAESADMMYMAEMLVKKIKNDPRINVQKHWKLISLMIGANDFCNEICSVSSPWSILENHKVDLLQVLRILRDNLPRTFVALIPPPHLKSLVDIRKEKSSFKCFVTTDVECSCLFGLAFQRYISIYYDIMRQWQILDMEIADYPEFQRNDFVVVAQPTLMNISIPLDSDGYSDMTYLSSDCFHVSQKTNARFANGLWNNLLETVGQ",
    "response#2": "SVHKLRPGDIDVIAAMGDSLTAGAGIFADNEFNPNLIGFALGDSLTTHKESQLNIAESGAESADMMYMAEMLVKKIKNDPRINVQKHWKLISLMIGANDFCNEICSVSSPWSILENHKVDLLQVLRILRDNLPRTFVALIPPPHLKSLVDIRKEKSSFKCFVTTDVECSCLFGLAFQRYISIYYDIMRQWQILDMEIADYPEFQRNDFVVVAQPTLMNISIPLDSDGYSDMTYLSSDCFHVSQKTNARFANGLWNNLLETVG",
    "response#3": "SVHKLRPGDIDVIAAMGDSLTAGAGIFADNEFNPNLIGFALGDSLTTHKESQLNIAESGAESADMMYMAEMLVKKIKNDPRINVQKHWKLISLMIGANDFCNEICSVSSPWSILENHKVDLLQVLRILRDNLPRTFVALIPPPHLKSLVDIRKEKSSFKCFVTTDVECSCLFGLAFQRYISIYYDIMRQWQILDMEIADYPEFQRNDFVVVAQPTLMNISIPLDSDGYSDMTYLSSDCFHVSQKTNARFANGLWNNLLETVG"
  },
  {
    "reference": "MGHRRRECRGGIRRRGDSVNPLPVLSATEAAGWDTAARTQYRVPSRVLMEAAGRACAGVLVKEFPEVVTRGALVVAGAGNNGGDGWVLARALHTAGVPVWVSALDPKTDDAIDNRALARVDGVRELGRDEPWPAAAMVVDALLGTGAAGPARGDVLAMAQRLVAYAAPVLAVDGPTGLDLSSGEAHGPVRAQVTITFGGPRRGHLIAREWCGKLVVVDIGLPPPDPSWPILVTDAWAGARLPRLHATMHKGDRGRVTVVGGAPGMTGAALHAARAALAAGAGLVKLVGSKEAVVAARAALPDLLTVESSLGGEPEPAAVEAVEWADALVVGPGLGREPPRGEFLAAVLQRKAVPTVLDADALMLFKGPAERPIVCTPHLGEFRALAGDALADAAANDRWGAATKAAQKLKATILLKGVPTVIADVRGPLHTIASGNPGLATGGSGDLLSGLIGAFLARGTAPVEAAALGAHALGRAAEHGARQWTARSLRPADVLAALPDIWRAWSDAKPQGPRVLTVLEPPETA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Mg(2+) is required for the proper folding and stability of the protein.2. The protein should have a high affinity for (6S)-NADPHX.3. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.4. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.5. The designed protein should be optimized for ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding, metal ion binding, NADPHX epimerase activity activity in order to improve nicotinamide nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "MIVAGSRNFLGAAYLAASAAGRVGAGLVTIAIPESLVPSVASKAIEPTFLPLPEAGPGIVANNAAHLILDSLNGYSSLLLVCGLGQAAETRQVVEELLLTDASLPPTVVDADGLNTLARIPDWWKLWPTDAIFTPHPGEMARLTSMSARNDRIAIARESASAWKKTVILKGAYTVASAPNGDAMLSPYSNPGLATAGTGDVLAGAIVGLLSQGVSIADAASLGVYLHGEAGERARTKLGDTGMLASDLLPQLLIIGGSKNYSGAPAYAALTAINFGCDLVITFTPSVVGDVIRSYSPNMIVRSSKGEWLTIKAYDEVSKLIDWADTILIGPGMGLEKETEELFVRILEKLNPIQKKLVLDADALKLVKNHLDLIKGKEVILTPHEGELKIIKAIDLPKYDEIEKRGDITFKLAKEINSIVLLKGPYDYISDGHSLKINKTGCPEMSIGGTGDVLAGLCASFLTTDNTAFKSACSAVFLNGYLGQYCLNNVGPRFTATDMIKNL",
    "response#2": "MRIEDVYIWDINAKWLGITPYQLMENAGAGVARAIEERFGTGLKIAVFSGTGNNGGDGFVAARHLSFENNVTLFLIGDEARIRSEEARHNWEILKKLDFVKIKVLKDSSQIKPLDLSGFDVIVDALLGAGTKGEPREPIRSAVEKINEYAGKTKIVSVDLPSGYPSGIWVKADFAVTFQWDKEEYEGFERVIVKIGYELVLGGSPGLTGAPVMASLAAARIGAGFVTCACASSIQPVLALKMTAITTLALPETADGGLDPDAALAALAPRLEKARALLVGPGLGRHPATQTFIRRLLTRTDLPTVIDADGLNALAGHLDDLAEQAAGRWILTPHAGEFRRLAGTDADLTDRIRTVQTCARRWNSVLLLKGLPGLVGTPDGRVYLNPTGNPAVATAGTGDVLAGLCVGLLAQGLPPARAAVAALYAGGAAADAYTTHRHPHTMTPLDLLEELPKG",
    "response#3": "MIVAGSRNFLGAAYLAASAAGRVGAGLVTIAIPESLVPSVASKAIEPTFLPLPEAGPGIVANNAAHLILDSLNGYSSLLLVCGLGQAAETRQVVEELLLTDASLPPTVVDADGLNTLARIPDWWKLWPTDAIFTPHPGEMARLTSMSARNDRIAIARESASAWKKTVILKGAYTVASAPNGDAMLSPYSNPGLATAGTGDVLAGAIVGLLSQGVSIADAASLGVYLHGEAGERARTKLGDTGMLASDLLPQLL"
  },
  {
    "reference": "MAKLANKKIVLGISGGIAAYKCAELVRRLKDHGCEIKVVMTESAKHFITPLTMQAVSGEIVSDSLLDPSAEAAMGHIEFAKWADLILVAPATSNIIAKMAAGIADDLLTTLLLATPAKVAVAPAMNQQMYAHPATQANLATLAQRNVAIWGPGKGEQACGDVGAGRMLEPHELVTLCTQPAHPQLLAGKTITITAGPTREPLDPVRFISNHSSGKMGYALAQAALSLGAHVNLISGPVTITPPAGANVIQIQSAQQLLNESLLLAPLSDVFIGCAAVADYRASAVADQKMKKQGDELILTLVKNPDVIAAIANLTEGRPYTVGFAAETQDVANYAKGKLSNKNLDMICANDVSQAGLGFNSDHNALTLFWLTEQKELAIETKSALALSVMTEVAKRL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein with FMN binding, metal ion binding is needed to enhance coenzyme A biosynthetic process, pantothenate catabolic process in a specific context.2. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.4. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.5. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal domain.6. The Proton donor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.7. The protein design should consider the endogenous proteins and enzymes present in the coenzyme A biosynthesis, Cofactor biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "KKIVLGISGGIAAYKSPELVRRLRDRGAEVRVVMTHAAKAFITPLTLQAVSGYPVSDDLLDPAAEAAMGHIELAKWADLVIMAPATADLLARVATGMANDLLTTACLATAAPIAAVPAMNQQMYRAAATQANLQTLASRGMLLWGPDSGSQACGDVGPGRMLDPLEIVALAHDGALAGKVAEAGKKILITGGSTREYIDPVRFITNNSSGKMGISIARVAKAMGAYTNLISGEILTNLPILDKYQKVETTEEMLKATKEAFKASDILIMAAAPVDFKPETISTTKIPKKKEITLKLLETPDILKEISKEKGNKIIVGFALQTENLENKALKKMKEKNMDIVVANRGKLKGEPVLLELHGKKVIITAGATIEAIDPVRFLSNHSTGKMGFAIARACVNAGADVTLVAGRAVHLPTPFGAKRLTVGNANDMLTACQSVMTNDSDTIFIATAAVADYKVKSIATQKIKKTSDQDGLTLELIKNPDILATMSHTFPSAIMVGFAAETQDAENYARTKLTAKNLDMIAVNDVSDKSIGFGSDNNAMTVVWKDGRAEELPLLSKEEVAGVILDRVERLLAGKG",
    "response#2": "KKIVLGISGGIAAYKSPELVRRLRDRGAEVRVVMTHAAKAFITPLTLQAVSGYPVSDDLLDPAAEAAMGHIELAKWADLVIMAPATADLLARVATGMANDLLTTACLATAAPIAAVPAMNQQMYRAAATQANLQTLASRGMLLWGPDSGSQACGDVGPGRMLDPLEIVALAHDGRLTGDGEGDAGLAGRHVVVTAGGTREPLDPVRFLGNRSSGKQGYALARAAVARGARVTLVAANVTLTDPAGADVVRVGTTEELRKATVEAAGGADVVVMAAAPADFRPATVAGQKIKKSDEGTAPTIELVTNPDIAAELGAAKRPGQLLVAFAAETHDALENARGKLVKKRADLIVVNEVGVDLVFGADHNTVTILSAAGEHTAIADKRILLGVSGGIAAYKSAELVRRLRDAGAEVRVVMTQAAREFITPLTMQALSGHPVHGDLLDPAAEAAMGHIELARWADLVLIAPTTAELMARLALGRGDNLLTTLVLATDAPVAIAPAMNQAMWRDPATQHNLQLLQQRGFHVFGPAAGEQACGDVGPGRMLEPSELAARLLALGSVRP",
    "response#3": "KRILLGVSGGIAAYKSAELVRRLRDAGAEVRVVMTQAAREFITPLTMQALSGHPVHGDLLDPAAEAAMGHIELARWADLVLIAPTTAELMARLALGRGDNLLTTLVLATDAPVAIAPAMNQAMWRDPATQHNLQLLQQRGFHVFGPAAGEQACGDVGPGRMLEPSELAHLLLGVTGSIAAYKAASLVRLFTTAGAEVRVLMTESASEFVTPLTLGTLSGGEVLTDVFPENEEGNWTKHVTLGAWADLFVVAPATAQTVAKLAHGFCDSMLTATALSARCPLLVCPAMDREMYRHPTVRDNLERLSEIGYEVMPAERGELASGLVGQGRMPEPEAIRSRVAEAALAVLGGASADLSGAAVLVTAGPTREPLDPVRVFTNRSTGTMGYAIAEAAAARGAAVTLVSGPTALDAPAEMEMVQVGTAAEMNEAVQARRDDADYVFAAAAVADYTPTDPSASKRKKEDGDLVLHLRRTPDILKMVGDHRRPDQVLVGFALETEDGLDNARRKLEDKHLDWIVLNNPTETGAGFGPATNRVTLDLLFSDGSVEHLPLLSKREIAGRVLDLVDALVSSR"
  },
  {
    "reference": "MNKYAIILAAGKGTRMKSKQPKVLHEVAGKTMLAHVLSSVSAIDTEKNIVIVGHEADRVIATLPKGTNFVKQNEQLGTGHAVKIAADLLSDKEGTTLVIAGDTPLITGETLQQLFDYHTSNKATATILTAIAENPTGYGRIIRGADNSVEKIVEQKDASEKEKAIHEINTGTYVFDNRALFSALNEITTDNAQGEYYLTDVIEIFKDLNRTVAAYTLENFDESLGVNDRVALSQAEKIMRQRINHQHMINGVTFIDPEHTYIDSDVTIGSETVIEGNVTIKGKTTIFNNVHITNGSRIVDSEIHSDTEIRNSTIEKSRMSVGSNIGPYAHLRPGTVLSEDVHVGNFVEVKGSTLGKGTKAGHLTYIGDATVGEKVNFGAGTITANFDGKNKFNTEIDDFAFIGSNTTIIAPLHIGKNGLTAAGSVINEDIPAETVAIARGRQVNKLGRAKKMPHYRGK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).2. The protein design should aim to mimic or enhance the function of natural proteins involved in cell wall organization, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "LLVIGGSQGARVLSDMVPEAIAGLPDEMRTRLSVSHQARAEDAERVIAAYASAGISAVVRPFFDDVPQRLADCQLVISRAGASSIADITVIGRPAILIPYAAATGDHQTANARALAESGAGVVLPESVLDAESLRRDMRDILSDSARATAMAAAALTLARPDAAQRR",
    "response#2": "LLVIGGSQGARVLSDMVPEAIAGLPDEMRTRLSVSHQARAEDAERVIAAYASAGISAVVRPFFDDVPQRLADCQLVISRAGASSIADITVIGRPAILIPYAAATGDHQTANARALAESGAGVVLPESVLDAESLRRDMRDILSDSARATAMAAAALTLARPDAAQRA",
    "response#3": "LLVIGGSQGARVLSDMVPEAIAGLPDEMRTRLSVSHQARAEDAERVIAAYASAGISAVVRPFFDDVPQRLADCQLVISRAGASSIADITVIGRPAILIPYAAATGDHQTANARALAESGAGVVLPESVLDAESLRRDMRDILSDSARATAMAAAALTLARPDAAQRA"
  },
  {
    "reference": "MFKAVNPRQNFPQMEEDILKLWQDKGVFKKSIENRRDGKRFTLYEGPPTANGRPGIHHVLSRVFKDVIPRYKVMKGYYAPRIGGWDTHGLPVELEVEKELGFTSKNDIEKYGIAEFNARCRSSVFKYVSEWNKLTERIAYWVDLDNAYITMDNNYIESGWWALKQMWDKGLVYQGHRVTPHCPRCGTSLSSHEVAQGYKDNTEDPSVFVKFEIAKESLAKAGLAKKWAYPADKPLYLLAWTTTPWTLPANTALAVSAADQYAILDMTDYYMILAKPRLSALKLAENPVAGECLGSDLSGLFYKPLFDPREFGIPVRNMQDNSETGVSEELLYPVITTSYVSMDDGTGIVHTAPAYGELDYESGVKYGLKFVHHVDLQGRITGSYPFAGKFVKEADKDISRNLKERGLMFRNERMHHTYPFCWRCDSPLIYYAKQSWYIRTTAVRDELIKGNQQINWYPEHIKDGRFGDWLENNIDWAFSRERYWGTPVPIWRCEKCGRTECVGGIDELKAKPNFKGMQEKLDIHRPYVDEWTYDCDKCGGNMKRVTEVMDCWYDSGAMPVAQYHYPFEPESRSIAKDGRFPADYICEAVDQTRGWFYSLHAISTLIFNRPCYQNVICLGHILDERGEKMSKSRNNVIQPATVLDKYGADAVRWYFYTAAPPGNARRFSEKLVGEVTRQFLLMLWNVYSFFVTYANIDSFTPSEKYLEGEVPELDRWILSELNQLVLDVDKGLDNYDPTQAGRRIEDFVGYLSNWYVRRSRRRFWKSEN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Incorporating a 'HIGH' region, 'KMSKS' region motif is critical for the successful design of the protein.2. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.3. A protein that can perform tRNA binding, zinc ion binding, ATP binding in cytoplasm to support isoleucyl-tRNA aminoacylation.4. The protein should be designed to have high stability and activity towards ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-   isoleucyl-tRNA(Ile). The designed protein sequence is ",
    "response#1": "ISEEIIKRNADAYRRIRNTARFLLANLFDFNPASDCIDAKELLELDRWALKRCQLLQEEIITAYENYHFHLIYQKIHNFCAVDMGSFYLDLIKDRQYTTAKDSIARRSCQTAMYHMVKAFTIWLAPILSFS",
    "response#2": "ISEEIIKRNADAYRRIRNTARFLLANLFDFNPASDCIDAKELLELDRWALKRCQLLQEEIITAYENYHFHLIYQKIHNFCAVDMGSFYLDLIKDRQYTTAKDSIARRSCQTAMYHMVKAFTIWLAPILSFL",
    "response#3": "ISEEIIKRNADAYRRIRNTARFLLANLFDFNPASDCIDAKELLELDRWALKRCQLLQEEIITAYENYHFHLIYQKIHNFCAVDMGSFYLDLIKDRQYTTAKDSIARRSCQTAMYHMVKAFTIWLAPILSFI"
  },
  {
    "reference": "MRASNYLFSTLRDAPTDAVVTSHQLMIRAGMIRQVSKGLYTWLPTGLKVFRKIENIVRDEMEKAGSLEVMMPGVQPAELWQETGRWQKYGPELLRLQDRHGREYCLGPTHEEVITELARNELTSYKQLPMNFFQIQTKFRDEVRPRFGVMRSREFCMKDAYSFHVGAESLQETYDIMHKAYCNVFDRIGLNYRPVRADTGSIGGAYSHEFHVLADSGEDDIAFSDSSDFAANIELAEAICLKEKADAPTQDITEVFTPDCKTIAKVAEFLKLDVTSTVKTMLIKAVDENDQPTIAALVLRGDHNINEIKVEKLAGVVIPFEFADEADIIAKTGCAPGSIGPKGLAEKGIRVIADRSAAVMSDFCAGANKDDYHFTGLNWERDCAEFEVADIRNVVEGDPSPDGQGTIVIKRGIEVGHIFQLGQQYAEALKATVLDENGKAQVMHMGCYGIGVSRIVAAAIEQNFDEKGILWPESIAPFDIAIVAMNYDKSEAVRTECDRLYAELKAKGLDVLLDDRKERPGVKFADCELLGIPHRLVVGDKGLEKGTLEYKYRKAGDNEDIAIADAVEFILSKK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The ideal protein would be one that plays a critical role in prolyl-tRNA aminoacylation through ATP binding within cytoplasm.2. For general function, the protein need meet that The misacylated Cys-tRNA(Pro) is not edited by ProRS.; The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala- tRNA(Pro); One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP; Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro); As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine The designed protein sequence is ",
    "response#1": "RFEDRGNRNFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFIMKDAYSFHTTQESLQATYDVMYQVYSNIFNRLGLDFRAVQADTGSIGGSASHEFQVLASSGEDDVVFSTESDFAANIELAEAIAIGERQAPTAEMCLVDTPNAKTIAELVEQFNLPIEKTVKTLIVKGADENQPLVALIIRGDHELNEIKAQKHPLVADPLEFADETEIKAKIGSGVGSLGAVNLNIPAIIDRTVALMSDFSCGANIDGKHYFNVNWERDVAIPKVFDLRNVVEGDPSPDGKGTLQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVVASAIEQFRLKDRKDAEFALGPTHEETITDLVRKNVRSYKDLPLNFYQIQTKFRDEARPRYGLIRGREFIMKDGYSFDVSEEDAKKTYEVMKEAYHKIFKELGLDYLMVEADVGAIGGKFSHEFVVKVPSGEAHIVYCEKCGYAANVEAAKFHHHKLPPEEPKPIEKVYTPDIKSVEDVANFLNVPLTKLVKTLIYKIDDKDFVAVLIRGDRELNETKLANLFKAIDVRMATKEELESLGIPEGFVGPIGLNLPIYADFSLKELYNIVVGANEKDYHYINANIDRDFKVSGFYDLATAKEGDPCPVCHLPLKETTGLEVGHIFLLGTKYSESMKAYFVDKDGKEKSIIMGCYGIGVSRLISAIVEQLAATKNSTPNAKTIAELVEQFNLPIEKTVKTLIVKGADENQPLVALIIRGDHELNEIKAQKHPLVADPLEFADETEIKAKIGSGVGSLGAVNLNIPAIIDRTVALMSDFSCGANIDGKHYFNVNW",
    "response#2": "RFEDRGNRNFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFIMKDAYSFHTTQESLQATYDVMYQVYSNIFNRLGLDFRAVQADTGSIGGSASHEFQVLASSGEDDVVFSTESDFAANIELAEAIAIGERQAPTAEMCLVDTPNAKTIAELVEQFNLPIEKTVKTLIVKGADENQPLVALIIRGDHELNEIKAQKHPLVADPLEFADETEIKAKIGSGVGSLGAVNLNIPAIIDRTVALMSDFSCGANIDGKHYFNVNWERDVAIPKVFDLRNVVEGDPSPDGKGTLQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVVASAIEQFGGANAGLTFSRDAVVPSHIYLVRGGFIRSLSAGIYEYLPLGLKVLRKIENIIRKHMDDSGALEVLLPILTPAELWKETGRWHVYGKELFRLKDRKDAEFALGPTHEETITDLVRKNVRSYKDLPLNFYQIQTKFRDEARPRYGLIRGREFIMKDGYSFDVSEEDAKKTYEVMKEAYHKIFKELGLDYLMVEADVGAIGGKFSHEFVVKVPSGEAHIVYCEKCGYAANVEAAKFHHHKLPPEEPKPIEKVYTPDIKSVEDVANFLNVPLTKLVKTLIYKIDDKDFVAVLIRGDRELNETKLANLFKAIDVRMATKEELESLGIPEGFVGPIGLNLPIYADFSLKELYNIVVGANEKDYHYINANIDRDFKVSGFYDLATAKEGDPCPVCHLPLKETTGLEVGHIFLLGTKYSESMKAYFVDKDGKEKSIIMGCYGIGVSRLISAIVEQS",
    "response#3": "FRLKDRKDAEFALGPTHEETITDLVRKNVRSYKDLPLNFYQIQTKFRDEARPRYGLIRGREFIMKDGYSFDVSEEDAKKTYEVMKEAYHKIFKELGLDYLMVEADVGAIGGKFSHEFVVKVPSGEAHIVYCEKCGYAANVEAAKFHHHKLPPEEPKPIEKVYTPDIKSVEDVANFLNVPLTKLVKTLIYKIDDKDFVAVLIRGDRELNETKLANLFKAIDVRMATKEELESLGIPEGFVGPIGLNLPIYADFSLKELYNIVVGANEKDYHYINANIDRDFKVSGFYDLATAKEGDPCPVCHLPLKETTGLEVGHIFLLGTKYSESMKAYFVDKDGKEKSIIMGCYGIGVSRLISAIVEQLMGREEGLRWPDAVVPSHIYLVRGGFIRSLSAGIYEYLPLGLKVLRKIENIIRKHMDDSGALEVLLPILTPAELWKETGRWHVYGKELFRLKDRKDAEFALGPTHEETITDLVRKNVRSYKDLPLNFYQIQTKFRDEARPRYGLIRGREFIMKDGYSFDVSEEDAKKTYEVMKEAYHKIFKELGLDYLMVEADVGAIGGKFSHEFVVKVPSGEAHIVYCEKCGYAANVEAAKFHHHKLPPEEPKPIEKVYTPDIKSVEDVANFLNVPLTKLVKTLIYKIDDKDFVAVLIRGDRELNETKLANLFKAIDVRMATKEELESLGIPEGFVGPIGLNLPIYADFSLKELYNIVVGANEKDYHYINANIDRDFKVSGFYDLATAKEGDPCPVCHLPLKETTGLEVGHIFLLGTKYSESMKAYFVDKDGKEKSIIMGCYGIGVSRLISAIVE"
  },
  {
    "reference": "MPEQRSEPGYPLVALLDRVATVPAAQNVPIRGLCNHAQDVRPGDLFVALAGTRTHGLRYLDEALAKGAAAVVWEPAPGIAPPAPASVPVIAVDELARRLGMVADRFFGHPSRGMTAIAVTGTDGKTSVSHFLAQALDAPWRGCGLIGTLGYGMYGHLVPGAHTTPDALRLHAELACQRAQGAAATVMEASSHALAQYRLDAVALDMAILTNLTRDHLDYHRNHGAYATAKRRLFSMPGLRHAVLNTDDAFGAALAAALPLSVNTMGYGIGASPLPDDRSVRATGIRARRGGLTFNVSTPQGTGMIESRLLGRFNVSNLLAALAALLTLDMPLSRALARINQTRGAPGRMEVRGTVGQPLVVVDYAHTPAALTHALTALREHCAGRLWCVFGCGGDRDAGKRPLMAQVAERHADAIIVTDDNPRGEPPARITADILTGFAAPDAVRVIHDRSEAIAGAVAAARAGDVVLVAGKGHEQVQIIGVEQRPFSDSQTVAMVLGEPPA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein requires Mg(2+) to activate a critical catalytic site.2. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.3. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.4. The protein design must feature the Meso-diaminopimelate recognition motif motif.5. The protein contains novel Mur ligase central, Mur ligase C-terminal domains that confer a unique function or activity.6. The protein design should aim to mimic or enhance the function of natural proteins involved in cell cycle, potentially with improved properties such as stability, specificity or selectivity.7. The designed protein should interact with enzymes and substrates within the peptidoglycan biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MYLKELLDAVSIEAVRGNTDVEVSGIAYRSDRVRPGDAFFCIVGMTTDGHSFAQDAIDRGAKVLVVERKVYLADATDVTEVVVSDTRKAMALAAANFYGNPSAKLKLVGITGTNGKTTTVTLLFNLFTALGYKCGLISTVQNHIGDVQEVSTHTTPDAASVQALLARMRDAKCAYVFMEVSSHAVHQHRIAGIHFAGGVFSNITHDHLDYHKTFDEYIKVKKKFFDDLPATAFALTNVDDKRGMVMLQNTRAEKHTYSLKAPASFKGKVLENNLTGLVMTVEGQEAHFRMIGTFNAYNLLAVYGVAVLLGLDKKTVLKKLGEVESIPGRLERYLFENGVCCIIDYAHSPDALRNVLQTLGEVVQGKIWSVFGMGGDRYAPNRPLMGEVASEFADELVITMDNPRSEDPAEIARQIEAGLKQGRRLKTHRVILDRPEAIHYALDHARAGDVVLVSGKGHEDYQILKDKTIHFDEREVVRELFSE",
    "response#2": "MKLRTEVSGIAYRSDRVRPGDAFFCIVGMTTDGHSFAQDAIDRGAKVLVVERKVYLADATDVTEVVVSDTRKAMALAAANFYGHPSQKMQMIGVTGTNGKSTVAYLVATMLNALNYKCAIFGTLGFGFPGELEQSSNTTLDVVYLNRYLDRYVQKGADHAVLEVSSIGVDQGRIDHLTFSVGAFTNLTRDHLDYHKTMEAYKEAKAAFLRRLNTQKIVINTDDPTGLEFASQKEGCTCYGIDSMTQVSALNGRRCISCEHLSFKDDCFNVSIKTSYGMLENQNVHLLGRFNVSNYLCAVGAGLLQGVPLETIIRGLESFKGVSGRLERYLFENGVCCIIDYAHSPDALRNVLQTLGEVVQGKIWSVFGMGGDRYAPNRPLMGEVASEFADELVITMDNPRSEDPAEIARQIEAGLKQGRRLKTHRVILDRPEAIHYALDHARAGDVVLVSGKGHETYQIIGEEKYHFDDLEQARLALENWNA",
    "response#3": "MALITGTNGKTSCTHFLADSLQKLQRSCGVIGTLGNGFYGHLKRAQLTTPDAIELQQLLAAFRAQHAQTVVMEVSSHRLAQKRLNGTEFSVAALTNLTRDHLDYHKTMDAYAKAKRSFFELPGVQHIVLNADDPYAQLWLTEFEKQLPISTYSLKRPQARWSHIPQVTVKKFDFNQQGLQAEIDTPWGDVFIENQFLMGAFNLSNLLLVLTTLLALEIPFTQALAALETLEGAPGRLERYLFENGVCCIIDYAHSPDALRNVLQTLGEVVQGKIWSVFGMGGDRYAPNRPLMGEVASEFADELVITMDNPRSEDPAEIARQIEAGLKQGRRLKTHRVILDRPEAIHYALDHARAGDVVLVSGKGHETYQIIGTEKLDFDDRVQARAAVRAMLEGQA"
  },
  {
    "reference": "MRVKGIKRNWRGLWGWGIMLLGMLMICNAADKLWVTVYYGVPGWKEANTTLFCASDAKAYDTEAHNVWATYACVPTDPNPQEVVLKNLTENFNMWKNNMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCEDAKGNYSNSSSIEEINGVRGEIKNCSFNVTIRRGDKAQGHYALFNRLDVVPVDTKNNKTNYMLISCNTSVITQACPKVSFEPIPIHYCAPAGYAILKCKDKKFNGTGLCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVVRSENFTDNVKTIIVQLNESIVINCTRPGNNTRKSIHIGPGKAFYTTGAIIGDIRQAHCNLSQGHWNNTLRRIATKLREQFGENKTIAFNQSSGGDLEIVMHTFNCGGEFFYCNTTKLFNSTWNGTENGTLTGSNDTSITLPCRIKQIINRWQEVGKAMYAPPISGQITCSSNITGLLLTRDGGPNENETETFRPGGGNMRDNWRSELYRYKVVRIEPLGVAPTKAKRRVVQREKRAVTLGAVFLGFLGTAGSTMGAASVALTAQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARILAVERYLRDQQLLGIWGCSGKLICTTAVPWNTSWSNKSLDDIWENMTWMQWEREIDNYTNIIYTLIEESQIQQEKNEQELLKLDSWASLWNWFSITNWLWYIKIFIMIVGGLIGLRIVFAVLSIVNRVRQGYSPLSFQIRLPAPRGPDRPEGIEEEGGERDRDRSGPLVNGFLALIWEDLRSLCLFSYHRL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must have at least one Human immunodeficiency virus 1 envelope glycoprotein Gp120, Retroviral envelope protein GP41-like domain.2. Include a signal peptide as an essential component of the protein.3. The designed protein should have at least Helical transmembrane domains to ensure proper localization.4. For general function, the protein need meet that Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Transmembrane protein gp41: Acts as a class I viral fusion protein; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain5. The protein design should include a Di-leucine internalization motif, YXXL motif; contains endocytosis signal motif to ensure proper function. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWKDTKTTLFCASNAKAYDTEVHNVWATHACVPTDPNPLETPLVNVTENFNAWKNDMVEQMHEDIISLWDQSLQPCVKLTPLCVTLDCVEYKHKHNASNSTVSTITVSNSNELLNCSFNMTTILRDKKEKMAALFYRLDVVPMEGQNSSKNSSYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCNDTKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEGEVVIRSQNITDNSKTIIVQLTEPVNITCIRPNNNTRKSWHIAPGQAFYATGEVIGDIRQAHCNVSRTKWNETLYKVATQLRKHFKNTTIIFANLSGGDLEVTTFTFNCGGEFFYCNTSALFNSSWNSTKNNTDIACNITLPCRIKQIINMWQRVGQAMYAPPIPGVIRCVSNITGILLTRDGGANGTNNETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPSRARRIVVERGKRALLGALFLGFLGAAGSTMGAAALTLTVQARQLVSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLRDQQLLGLWGCSGKLICPTTVPWNSSWSNKSQDQIWQNMTWLQWDKEISNYTDIIYNLIEESQNQQEKNEQDLLALDKWADLWNWFDISQWLWYIRIFIMIVGGLIGLRIVFAVLSMIKRVRQGYSPLSLQTLLPVPRGPDRPEGTAEEGGERDRDGSGRLVGGFLTLIWVDLRSLCLFSYHRLRD",
    "response#2": "MRARRVQARQLLSGIVQQQNNLLRAIEAQQRMLQLTVWGIKQLQARVLAVKRYLRDQQLLGIWGCSGKLICTTNVPWNASWSKNKSMDDIWHNMTWMQWEREIDNHTSLIYSLLETAQYQQEKNEQELLELDRRGLLTLGALFLGFLGAAGSTMGAASIALTVQARQLLSGIVQQQNNLLRAIEAQQRMLQLTVWGIKQLQARVLAVKRYLRDQQLLGIWGCSGKLICTTNVPWNASWSKNKSMDDIWHNMTWMQWEREIDNHTSLIYSLLETAQYQQEKNEQELLELDKWASLWNWFDITHWLWYIKIFIMVVAGLVGLRIVFTVLSIVNRVRQGYSPLSFQTRPPAPRGLDRPEGIEEEGGERDRDTSGPLVDGFLTLIWVDLRSLCLFSYHRLRDLI",
    "response#3": "GEIKNCSFNMTTELRDKKQKVYSLFYKLDVVQMNSSQYRLINCNTSAIKQACPKVSFEPIPIHYCAPAGFAILKCRDEEFDGTGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEKRVMVRSENITNNVKNIIVQFNETVNITCIRPNNNTRKSVHIGPGQAFYATGDIIGNIRKAHCHVNKTEWNRMLAKAAQQLINHFNKTITFKNSSGGDLEITSHSFNCGGEFFYCDTSSLFNITWDRSNVTQELNNTKLNDTIILQCRIKQIINMWQRAGQAMYAPPIPGIIKCESNITGLILTRDGSENETETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKARRRVVEREKRAVGLGALFLGFLGAAGSTMGAASIALTVQARQLLSGIVQQQNNLLRAIEAQQRMLQLTVWGIKQLQARVLAVKRYLRDQQLLGIWGCSGKLICTTNVPWNASWSKNKSMDDIWHNMTWMQWEREIDNHTSLIYSLLETAQYQQEKNEQELLELDKWASLWNWFDITHWLWYIKIFIMVVAGLVGLRIVFTVLSIVNRVRQGYSPLSFQTRLPTQRGLDRPEGIEEGGGEQDRDRSVRLVSGFLALAWDDLRSLCLFCYHRLRDFILIAVRTV"
  },
  {
    "reference": "MSLQISILTPERPFWNGQADEIILPTETGEMGVLKNHAPIITGLNVGAMLIRGGQASGSKDEWNSYAIMGGFALVKQNQVTILANEAVSAENINPEEAKDAFETAKANLEKAEGVKEKVEANFAYKRAKARYQVVKVLKKI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.2. A protein that localizes to chloroplast thylakoid membrane and can perform ATP binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "RGGKIGLFGGAGVGKTVLIMELINNVAKAHGGFSVFAGVGERTREGNDLYREMIESGVIKLGDKRGESKCTLVYGQMNEPPGARARVALTGLTVAEYFRDVEGQDVLLFVDNIFRFTQANSEVSALLGRIPSAVGYQPTLATDLGGLQERITTTTKGSITSVQAVYVPADDLTDPAPATTFAHLDAST",
    "response#2": "RGGKIGLFGGAGVGKTVLIMELINNVAKAHGGFSVFAGVGERTREGNDLYREMIESGVIKLGDKRGESKCTLVYGQMNEPPGARARVALTGLTVAEYFRDVEGQDVLLFVDNIFRFTQANSEVSALLGRIPSAVGYQPTLATDLGGLQERITTTTKGSITSVQAVYVPADDLTDPAPATTFAHLDAI",
    "response#3": "GIKVVDLLAPYQRGGKIGLFGGAGVGKTVLIMELINNVAKAHGGFSVFAGVGERTREGNDLYREMIESGVIKLGDKRGESKCTLVYGQMNEPPGARARVALTGLTVAEYFRDVEGQDVLLFVDNIFRFTQANSEVSALLGRIPSAVGYQPTLATDLGGLQERITTTTKGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRSIAELGIYPAVDPLDSTS"
  },
  {
    "reference": "MNNQSKRLWIEPIQGSRRKSNFFFASILFGGALGFFLVGFSSYLGRNLLPLLSSQQIIFVPQGIVMCFYGIAGLFFSSYLWCTIFFNVGSGYNQIDEKTGIVCLFRWGFPGRNRRIFLRFPLKNVHMIKMEVQENLFSSRHILYMKVKGLPDIPLARTGENLNLKEMEQKAAELARFLHVSIEGF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a specific function within the chloroplast thylakoid membrane. The designed protein sequence is ",
    "response#1": "ELQRYTDSNNGFTLLIPSSYTKVEKAGANALFEELNNGSNNIGVVVSPVRIKSLDQFGSPQFVADKLINAEKRKESTKEAEVVSVGERAGLGQQVYEFEYKIDSTRGGIKRVFSAAFVSSNKLYLLNVVHSDKPENPLDSSTRMSLEQVLHSFI",
    "response#2": "ELQRYTDSNNGFTLLIPSSYTKVEKAGANALFEELNNGSNNIGVVVSPVRIKSLDQFGSPQFVADKLINAEKRKESTKEAEVVSVGERAGLGQQVYEFEYKIDSTRGGIKRVFSAAFVSSNKLYLLNVVHSDKPENPLDSSTRMSLEQVLHSFR",
    "response#3": "ELQRYTDSNNGFTLLIPSSYTKVEKAGANALFEELNNGSNNIGVVVSPVRIKSLDQFGSPQFVADKLINAEKRKESTKEAEVVSVGERAGLGQQVYEFEYKIDSTRGGIKRVFSAAFVSSNKLYLLNVVHSDKPENPLDSSTRMSLEQVLHSFR"
  },
  {
    "reference": "MLHSSIYWKNLINTFESGCFSIAEVKFIFSNIHIYKNICNPYKNYQLPELARNLQSSKKLDNAILKQDKKNIIDILSPISGIFYSSSKPGASPFVAVGSVVSKGQTLCIIEAMKTMNEIESDSIGKIHQICARNGDFVTKNQVLMKIILEQS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should contain one or more Biotinyl-binding domains that are essential for its function.2. The designed protein should have a specific function within the chloroplast.3. A protein that is capable of catalyzing reactions within the fatty acid biosynthesis, Lipid metabolism pathway with high efficiency and specificity.4. For general function, the protein need meet that This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA. The designed protein sequence is ",
    "response#1": "PPDRLGEPETSDPWQMVRRARELERPTTLDYIHHMYDGFKELHGDRVSGDCPAMIAGFGHLAGRPVGIIGQQKGHDAAELARRNFGMASPAGYRKAARLMALAEKLRIPIITLIDTPGAYPGVEAEANGQMNAIAASLRLMSGLRVPLIAVLTGEGGSGGALALALADRVLALEHAVYSVISPEGCASILWRSQDAAPTAARALKVTPSDLLRLGIVDDMVPEPPGGAQRDHARAAEILRARLTAALDAA",
    "response#2": "LWSKCDSCGAALHKKQLEDHLYTCPHCGFHFRISPDLYFSFLFDDGKWEEFDAQLRAADPLKFVDTKPYPERVRSTMQKSGKSEACRNATGKLGGSDAVISGMDFGFIGGSMGSVVGEKIARAADKSVELNAPLIVISQSGGARMMEGAFSLMQMAKTSARLTRLGERGIPFISLMTDPTMGGISASFAMLGDLNISEPKALIGFAGPRVIRDTIKRDLPEGFQRAEFLQKHGFVDTIVHRKDLRAQLIKLLGHMN",
    "response#3": "MQKSGKSEACRNATGKLGGSDAVISGMDFGFIGGSMGSVVGEKIARAADKSVELNAPLIVISQSGGARMMEGAFSLMQMAKTSARLTRLGERGIPFISLMTDPTMGGISASFAMLGDLNISEPKALIGFAGPRVIRDH"
  },
  {
    "reference": "MENRYAIILAAGKGSRMKSSLYKVLHPVAGKPMVEHVIEQVEAINVDEIVTIVGFGAETVKDHLGERSKYALQTEQLGTGHAVLATADLLKDKKGTTLVICGDTPLLTSDTLDKLVKHHEETNAKATILSAIARDASGYGRIVRNDNNLVEKIVEHKDASEAELLINEFNTGTYCFDNEFLFEALTKVGNDNAQGEYYLPDVISILKEQGEIVTAFVMDSEDESMGVNDRVALAKASQLMNARINEAHMRNGVTFVDPSNTYIESDVVIGHDTLIEPGVILRGKTIIGDNCYISTNSDIKDSVLGNHVKVHSSNIKESTIRDGADVGPYAHIRPNSEIHENAHIGNFVEIKKATIGKESKVGHLTYVGDATLGKDINVGCGTVFVNYDGKNKFHTTIGDHSFIGSGTNLVGPVTLGRNSATAAGSTITEDVPEDALGIARGRQSNLEGYAKKMPYNE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have glucosamine-1-phosphate N-acetyltransferase activity, magnesium ion binding within cytoplasm to regulate UDP-N-acetylglucosamine biosynthetic process, regulation of cell shape, cell wall organization, cell morphogenesis, lipid A biosynthetic process.2. The protein contains novel Nucleotidyl transferase domains that confer a unique function or activity.3. Ideal protein should have a binding affinity for key enzymes in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, Nucleotide-sugar biosynthesis pathway to enhance the overall efficiency of the pathway.4. The Mg(2+), acetyl-CoA, UDP-N-acetyl-alpha-D-glucosamine binding site should be stable and able to withstand changes in environmental conditions.5. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate. The designed protein sequence is ",
    "response#1": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVISPASDDPFGYGRIERDEEGRVTRIVEQKDASEEEQRITECNTGILLMPVPHLKRWLGELTNDNAQGEYYLTDLGVTFIDPGSVFVHAGVTIGRDSVIYPNVYLEGNTKIGRKATIFPNVRIVNADIGAGVVIKDSTVIEESQVRKGASVGPFAHLRPGSQVGDRAKVGNFVELKKAVIGKDSKASHLSYLGDTRIGRGVNIGAGTITCNYDGTHKHTTVLEDGVFVGSDTQLVAPVKVGRGSYIGAGSTITKDVPPGALALSRTVQREVEGWQRPRKRP",
    "response#2": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLLDDPTGYGRLIRDGAGRVAAIVEQKDASAEQLAIREVNTGFLAVPILHLRGWLARLDNRNAQGEYYLTDVIGLAVADGVPVASAQPAHVWETLGVNSKRQLADLERAHQRDLAEALMLAGVTLADATRIDVRGEISAERDTFIDVNTVFEGNVRIGEGSKIGPNCVLKNVVLGKNCEVYANSVLEGCEIAENCQIGPFARLRPGTKLAKHCKIGNFVETKNAVFDESSKANHLTYLGDVTLGKNVNVGAGTITCNYDGAYKHQTVIEDGVFIGSDTQLVAPVTVGENATIGAGSTIRKNVPAGELTLTVSTPPRKIPGVSILDPHTTWIDVTVTVERDAVLDQNTQLQGATAVGAGAVVGPDVTLIDTTVGEGATVVRSHAVGAEVGPQATVGPYAYLRPESRLARKAKVGTFVETKKATIGEGSKVPHLSYVGDATIGEQSNIGAATVFVNYDGVRKHHTTIGSHARTGADNMFVAPVEVGDGAYTAAGSVIIADVPPGAMAVARGQQRNISGWTRPVREPKP",
    "response#3": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLTAEPKDPFGYGRIIRENGAVVAIREEKDATPEEQALSFCNSGIMGFRGRDCLDLLEQVGNSNAKGEYYLTDLVGIARKHGKAALVVEGEAAEAMGVNDRVQLAAAEEMMQLRKRQALLDAGVTLADATRIDVRGEISAERDTFIDVNTVFEGNVRIGEGSKIGPNCVLKNVVLGKNCEVYANSVLEGCEIAENCQIGPFARLRPGTKLAKHCKIGNFVETKNAVFDESSKANHLTYLGDVTLGKNVNVGAGTITCNYDGAYKHQTVIEDGVFIGSDTQLVAPVTVGENATIGAGSTIRKNVPAGELTLTVSTLARAEALFQDVRREDFMKAGVTLRDPKTVWFSWDTEIGKDVTIFPNVVFGPGVSIADKVEIRAFCDIEDAVIGEGASIGPFARIRAGAELGRDVHLGNFVEVKKSRIGDGTKAGHLSYLGDAEIGTKTNIGAGTITCNYDGVNKDKTIIGNNVFIGSNASLVAPVSIGDGAYTASGSVITEDVPADALALGRARQVNKKGLAVRLMQALKAAKAKK"
  },
  {
    "reference": "MKISALQKERAKYQPKLPNSLTGTVQLVNGEKTESVADQGDIAKLFPNTYGMPLITFEKGNGKQSSAAVNVGVILSGGQAPGGHNVISGIFDGIKKINSNSKLYGFLGGPGGLVDHKYVELTSDIIDEYRNTGGFDIIGSGRTKLEEEWQFDKGLEIAKKLDLKALVVIGGDDSNTNACVLAEYYAAKDAGVQVIGCPKTIDGDLKNEMIETSFGFDTATKVYSELIGNIQRDSNSAKKYWHFIKLMGRSASHIGLECALKTQPNITLISEEVAEKKQTLNEIVDYMAGIVAQRAANGDNFGVALIPEGLIEFIPEMKVLISELNDLLAEGFESEKIFRELEKEQRNSWVAEKLSSTSSKVFKSLPSGIATQLTLDRDPHGNVQVSLIETEKLLGEMVKTKLQKMKNNGEFKGKFGTQYHFFGYEGRCAAPSNFDADYCYSLGFTASVLIAEGKTGYMSSVRNLTAPAEQWIAGGVPITMMMNMERRHGHVKPVIQKALVDLEGAPFKYFASKRDEWAMNTCFVYPGPIQYFGPTEVCDQTTETIKKENC",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The substrate binding site should be located in a region of the protein that is accessible to the ligand.2. The protein design must be able to perform metal ion binding, diphosphate-fructose-6-phosphate 1-phosphotransferase activity, 6-phosphofructokinase activity in order to contribute to fructose 6-phosphate metabolic process.3. The designed protein should contain one or more Phosphofructokinase domains that are essential for its function.4. A protein that can modulate the overall flux of metabolites through the D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 3/4 pathway, enhancing or suppressing its activity as needed.5. For general function, the protein need meet that Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis; Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis; Consistently, PPi-PFK can replace the enzymes of both the forward (ATP- PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions. The designed protein sequence is ",
    "response#1": "VAILVGGGPAPGINGVIRSITIEAVNSGLRVIGIYDGFKWLARGDSSRTTELHTENVSRIHFLGGSILRTSRDNPTETPAKMEATVRALTQLEVDYLITIGGDDTAYSSQRLHELVGVQIQIAHVPKTIDNDLPLPGDMPTFGYETARHVGAEIVRTLMEDAQTTNRWYYVVSMGRKTGHLALGIGKAAGATLTIIGEEFRNRKISFTTVCDMLETSAIKRMAMGRPYGVAVLAEGILDRIDKNELMRVADLDYDAHGNIRFSEIDLGRMLKNEVSRRMKERGIPATLVAKDIGYELRCASPIPFDREYVQDLGYAAVRYAQAGGVTAVINASAAAVIEAAREHPQAIGTVYGARNGILGVLSETLVDTTPLTPAELAQLALTPGGAFGSCRFDLDPAEANPAQYDRLFEVFASYRIGAFLYNGGNGSMDIVARVAAAARARAYPLCCIGVPKTVDNDLEGTDCSPGFGSAAKYLATAMRESGLDLAAMSSRRGRVFVMEVMGRNAGWLAAATALAARNEDEPPHIVLLPEVAFDEEAFLARVKAVVERLGYCAVTVAEGIRRADGTLVMEQSHDALGHVQLGGAGQCVARLVSARFDYKHGFCVTRVLVEEGRCSGCGNCAEVCPTGALRIIDYAKAVEAHEKNG",
    "response#2": "LAVVLSGGQAPGGHNVIAGLYDGLKKGNKKSRLLGALGGPKGLIDGKFTEINDDLMDAYRNTGGFDMIGSGRDKIEKPEDLEKVVAVVKANGINGVVIIGGDDSNTNAAVLAEYFQSLKLDVSVVGCPKTIDGDLKNEHIETSFGFDTACKTYSELIGNIGRDANSAKKYWHFIKLMGRSASHITLECALQTQPNVCLIGEEVAAKKQTLDQIVESVASVVAKRAEQGNNFGIVLVPEGLIEFIHVPIKAGGESRIADAIAQSGGVTSVINASACGVIETARKHKSEIGKVYAGRNGIIGALREELIDTSKESRSAIHGLLTTPSGAFGSCRHKMKTIEENRAEYERLIEVFKAHNIGYFLYNGGGDSADTCLKVSQLSEAMGYPLQAIHIPKTIDNDLPHTDNCPGFGSVAKYIAISTREAALDVASMCESSTKVFILEVMGRHAGWIAASGGLAAEQEGDAPHIILFPEIAFNREKFLAKVKRTVKKNGYCVIIASEGAQYKDGSFLAESGLKDAFGHSQLGGLAPNLAQMIKDEHGYKYHWAVADYLQRAARHIASQTDLDQAYAVGEAAVLAVVLSGGQAPGGHNVIAGLYDGLKKGNKKSRLLGALGGPKGLIDGKFTEINDDLMDAYRNTGGFDMIGSGRDKIEKPEDLEKVVAVVKANGINGVVIIGGDDSNTNAAVLAEYFQSLKLDVSVVGCPKTIDGDLKNEHIETSFGFDTACKTYSELIGNIGRDANSAKKYWHFIKLMGRSASHITLECALQTQPNVCLIGEEVAAKKQTLDQIVESVASVVAKRAEQGNNFGIVLVPEGLIEFI",
    "response#3": "LAVVLSGGQAPGGHNVIAGLYDGLKKGNKKSRLLGALGGPKGLIDGKFTEINDDLMDAYRNTGGFDMIGSGRDKIEKPEDLEKVVAVVKANGINGVVIIGGDDSNTNAAVLAEYFQSLKLDVSVVGCPKTIDGDLKNEHIETSFGFDTACKTYSELIGNIGRDANSAKKYWHFIKLMGRSASHITLECALQTQPNVCLIGEEVAAKKQTLDQIVESVASVVAKRAEQGNNFGIVLVPEGLIEFIRTPQEFRRIARRTEESRVGMLTGGGDCPGLNAVIRGAVRKGVTAHGHEFVGFRDGWRGPLEGDTMTLGIDEVRGILPRGGTILGSSRTNPFAIENGVERIKDNLAAAGCDALIAIGGEDTLGVATKLADLGVNVVGVPKTIDNDLSGTDFTFGFDTAVNIAMEAIDRLHTTAESHHRALVVEVMGRHAGWIALHSGLAGGANIILIPERPFDIDKVCAQVESRFATHYSPIIVVAEGATPLEGTMELVSGEKDAFGHVRLGGIGDRLAHEIEERTGREARAVVLGHIQRGGTPTAFDRWLATRFGLHAIQALLEGKRECMVAATKIGILTGGGDCPGLNAVIRAVVRRSHALGYDVLGIRNGWAGLINGHVEPLTDFSVVGILPRGGTILGTSRTNPLKQVEHFQKLRSNWQRYGLNYLVAVGGEDTLGVAHKLFTQEGYPIVGVPKTIDNDLAGTDYTFGFDTAVQIVTEAIDRLHTTAESHKRVMVVEVMGRHAGWIATHSGMAGGADCILVPERRFSLDEVCALIRKRIERGREFAIVVVAEGAAPDEGSPPITKSGHVDEFGHPQLGGVGDWLGRQIEQRMGIETRVTVLGHVQRGGTPTAYDRVLATRFGLKAIEL"
  },
  {
    "reference": "MGKEKVHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYITIIDAPGHRDFIKNMITGTSQADCAVLVVACGTGEFEAGISKNGQTREHALLAQTLGVKQLIVACNKMDSTEPPFSEARFTEITNEVSGFIKKIGYNPKAVPFVPISGFNGDNMLEVSSNMPWFKGWAVERKEGNASGKTLLEALDSIIPPQRPTDRPLRLPLQDVYKIGGIGTVPVGRVETGIIKPGMVVTFAPQNVTTEVKSVEMHHESLPEAVPGDNVGFNVKNVSVKDIRRGSVCSDSKQDPAKEARTFHAQVIIMNHPGQISNGYTPVLDCHTAHIACKFNELKEKVDRRTGKKVEDFPKFLKSGDAGIVELIPTKPLCVESFTDYAPLGRFAVRDMRQTVAVGVIKSVEKSDGSSGKVTKSAQKAAPKKK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis.2. The designed protein should have translation elongation factor activity, translation factor activity, RNA binding, GTP binding, GTPase activity within cytoplasm to regulate translational elongation.3. The presence of tr-type G domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "EKTRNIGIMAHIDAGKTTTTERILYYTGVTHKIGEVHEGAATMDWMEQEQERGITITSAATTCFWGDHRVNIIDTPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEPQSETVWRQADKYRVPRIAFINKMDRVGADFFRGVGMIRDRLKANPVPIQLPIGAEDTYRGVVDLVEMKAIIWDEESLGAKYHEAEIPADLAEMAQEYREKLIEEIATFDDALMEKYLGGEELTTDEIKAAVRKATIDIQICPVICGSAFKNKGVQNLLDSVVAYLPSPADIPPVHGDKVDAFSAITHKDAAYAYGVRLVAKLRELIPRQAFEVPIQAAIGSRVIARETIRAIRKDVLAKCYGGDISRKRKLLEKQKEGKKRMKMVGSVEVPQEAFIAVLD",
    "response#2": "EKTRNIGIMAHIDAGKTTTTERILYYTGVTHKIGEVHEGAATMDWMEQEQERGITITSAATTCFWGDHRVNIIDTPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEPQSETVWRQADKYRVPRIAFINKMDRVGADFFRGVGMIRDRLKANPVPIQLPIGAEDTYRGVVDLVEMKAIIWDEESLGAKYHEAEIPADLAEMAQEYREKLIEEIATFDDALMEKYLGGEELTTDEIKAAVRKATIDIQICPVICGSAFKNKGVQNLLDSVVAYLPSPTDIPSVDGEIPEGRPKDTKVVAKKLAELGMDYEIARGIVDIYNGNMFLDNTKGLQYLNEVMDLLVDGFHQAMDEGPLAREPVMKVIVRLVDAKIHEDNVHRGPAQIYPAIRTAIHCAMMKSAILEPLMKVEVEVPEDYMGDVIGDLNRRRGQVTNMGDRSGNKIVDAFVPLAEMFGYSTDLRSATQGRATYSMEFDHYEEVPKNVSEEIMHGDKVDAFSAITHKDAAYAYGVRLVAKLRELIPRQAFEVPIQAAIGSRVIARETIRAIRKDVLAKCYGGDISRKRKLLEKQKEGKKRMKMVGSVEVPQEAFIAVLKDTKVVAKKLAELGMDYEIARGIVDIYNGNMFLDNTKGLQYLNEVMDLLVDGFHQAMDEGPLAREPVMKVIVRLVDAKIHEDNVHRGPAQIYPAIRTAIHCAMMKL",
    "response#3": "EKTRNIGIMAHIDAGKTTTTERILYYTGVTHKIGEVHEGAATMDWMEQEQERGITITSAATTCFWGDHRVNIIDTPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEPQSETVWRQADKYRVPRIAFINKMDRVGADFFRGVGMIRDRLKANPVPIQLPIGAEDTYRGVVDLVEMKAIIWDEESLGAKYHEAEIPADLAEMAQEYREKLIEEIATFDDALMEKYLGGEELTTDEIKAAVRKATIDIQICPVICGSAFKNKGVQNLLDSVVAYLPSPRDLKDGLPGEIPEGRPKDTKVVAKKLAELGMDYEIARGIVDIYNGNMFLDNTKGLQYLNEVMDLLVDGFHQAMDEGPLAREPVMKVIVRLVDAKIHEDNVHRGPAQIYPAIRTAIHCAMMKSVILEPIMKVEVTVPEEYMGDIMGDVNSRRGRIEGMEQRAGAEVIRAFVPLSEMFGYATSLRSRTQGRGVFTMQFDHNEEVPKNIQEKILG"
  },
  {
    "reference": "MSTESMIRDVELAEEALPKKAGGPQSSRRCLCLSLFSFLLVAGATALFCLLHFGVIGPQREEHPIGALLPQTLGLSSRTSNDKPVAHVVADQQTVGQLQWQSKVANALLANGVQLVDNQLEVPSDGLYLIYSQVLFRGQDCPQTQLLLTHTIKRFAVSYPDKVNLLSAIKSPCQRETPEGMEAKPWYEPIYLGGVFQLEKGDRLSAEINLPDYLDFAESGQVYFGLIAL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a specific function in regulation of biological quality and should be localized to extracellular space in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "LNPHNAARRQVGVGPMTWDNRLAAYAQNYANQRIGDCGMIHSHGPYGENLAAAFPQLNAAGAVKMWVDEKRFYDYNSNSCVGGVCGHYTQVVWRNSVRLGCARVRSNNGWFFITCNC",
    "response#2": "KSEHSAAAKTGGCFPGDGLVTLESGERRAVSTLRPGDRVLAMDSTGRPTFSDFLTFLDKDPSSLRHFHVIETRDPPRQLTLTATHLLFLAKNFSRPLTDFQPLFASSVQPGQYVLVAGALGLQPAQVTAVSTALDTGAYAPLTRHGTVVVDGVVASCFAVVQEQQLAQLVFWPLRLYHSLVRWPGEAQQDGVHWYSHLLYRLAQLLR",
    "response#3": "GDFKLQLMIIETDIERLNYLVRLYLRTRLAKIDKFTIHYINETSNDDPTNDRSLLSPEETEYMHKHFKILTQLYNNR"
  },
  {
    "reference": "MDTADTNPASMQTLAGTLAAPDTLRVLTCGSVDDGKSTLIGRLLYDSKLLLQDQIADLERDSRRHGTTGGDIDFALLVDGLEAEREQGITIDVAWRFFATPRRRFIIADTPGHEQYTRNMATGASTAELAILLVDAEKGLSRQTRRHALICSLMGIKQVVLAVNKMDLVGYDEAVFEKIAADFAAFAGRTRIESVTPIPLSAREGDNVTGPSPRLPWWKGPALLPFLEEAPSAPGLVETPLRFPVQWVCRPDHTFRGYAGTISSGRIAVGDEVVVAASGKKSRVTAIDIGENALQEAVAGQSIMLRLADEVDIARGDVLVPAENRPEVTDQIAAHLVWMAEEPLLPGRPYWLKAANRMVSAQVSELKHHLSVDTLEETAGKVLELNEIGFANLSLAHPIAFDPYGENRETGGFILIDRFTHSTVAAGMVDFSLRRAANIHWQSLDVDRNARARLKNQKPTVLWFTGLSGAGKSTVANWVEKKLYAAGRHTITLDGDNVRHGLNRDLGFTDADRVENIRRVAETAKLMTDAGLIVLVSFISPFRSERQMARQLMMEGEFVEVFVDTPLDIAEKRDPKGLYAKARAGQLKNFTGIDSPYQMPENAEITVDTTSASPEELADEIIAYLRQNGRLS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Ideal protein should have a binding affinity for key enzymes in the Sulfur metabolism pathway to enhance the overall efficiency of the pathway.2. The protein must exhibit the following characteristics: With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway.3. The protein must be able to GTPase activity, ATP binding. The designed protein sequence is ",
    "response#1": "LLRLLTCGSVDDGKSTLIGRLMFDSKMIYEDQLAALERDSKRIGHAGEDIDYALLLDGLKAEREQGITIDVAYRYFSTNKRKFIIADTPGHEQYTRNMVTGASTANLAIVLVDATKGVITQTRRHTFLVSLLGIKHVVLAVNKMDLVDFSESRFHEICEEYKQFVSQLDIPDVQFIPLSALKGDNVKSLLRFITCGSVDDGKSTLIGRLLWDSKLIFEDQLAALQADSKKVGTQGGDIDYALLLDGLQAEREQGITIDVAYRFFSTDKRKFIVADTPGHEQYTRNMATGASNADIAVILVDGRKGILTQTKRHSYIVSLVGIRKVVVAVNKMDLVDYSKERFLEIEEEYRAFAKDLGFEDITCIPISALKGDNMIAASEQTHWYHGPTLLSYLETVPVASELLPLLDQFYASGLRFPVQLVSRPDRTFRGYSGQLSSGILRKGTEIMALPSMQKSRVKSIVTFDGELEEARPGQAITITLEDERDISRGDMLVHPNNKPLSAHVIWMAEDALLPGRSYLMKIGARTLAASVTDIRHKINVNTFEQTAAKTLALNEIGLCNISLAAPAAFEAYGANRTMGAFILIDRMTNATVGAGMILHGLRRADNIHWQALDVDKAARAAQKGQSPKLLWFTGLSGSGKSTLANALEVKLHALGRHTYILDGDNLRHGINKDLGFTDADRVENIRRVAEVAKLMADAGLIVLTAFISPFRAERDMVRALVGEGEFIEVFVDTPLAVAEERDPKGLYKKARSGQLPNMTG",
    "response#2": "RFITCGSVDDGKSTLIGRLLYDSRMIFDDQLAALEADSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFATEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSYLAHLIGIRHVVLAVNKMDLVGYDQAIYDAIVADYAAFAKSIGIAAFTPIPISGFKGDNVTAPSSNTPWYAGPSLIEHLETIELDRFLTCGSVDDGKSTLIGRLLSDTKQIFEDQLAALERDSRKHGTTGDDVDFALLVDGLEAEREQGITIDVAYRFFATPKRKFIVADTPGHEQYTRNMATGASTADLAIVLIDARQGVLRQTRRHSVIASLLGIRHIVLAVNKIDLVDFDKAVFDRIVADYAQFSKTLGFRSVAAIPMSARYGDNVSKRSQNMEWYDGPTLIEHLETVAVDRSELRFITCGSVDDGKSTLIGRLLFEANGLFQDQIATLERDSRRFGTTGGEIDFALLLDGLEAEREQGITIDVAYRFFSTARRAFIVADTPGHKQYTRNMATGASNADFAVLLVDARRGLLEQTRRHAAIVSLLGIRHVALAINKIDLVGFSQEVFDEITVAFKLFAAPLGFALITAIPLSARFGDNVAAPSTRTPWYAGPTLLDLLESVDVEPLRFPVQLVSRPDRTFRGYSGQLSSGILRKGTEIMALPSMQKSRVKSIVTFDGELEEARPGQAITITLEDERDISRGDMLVRPGNRPQVADQFEATIVWMADDALLPGRGYWLKLGTQTVTAQVHAPKYQVNVNTLEQVAAKTLDLNAIGVANLVTDKPIVFEPYADSRELGGFILIDKLTNATVAAG",
    "response#3": "RLLTCGSVDDGKSTLIGRLMFDSKMIYEDQLAALERDSKRIGHAGEDIDYALLLDGLKAEREQGITIDVAYRYFSTNKRKFIIADTPGHEQYTRNMVTGASTANLAIVLVDATKGVITQTRRHTFLVSLLGIKHVVLAVNKMDLVDFSESRFHEICEEYKQFVSQLDIPDVQFIPLSALKGDNVVDASQKMAWYKGKCMLDFLETVHISKELLRFLTCGSVDDGKSTLIGMLLYESKMIYEDQLAAVQAASATHGTTGGGFDPALLTDGLKAEREQGITIDVAYRYFSTAKRKFIIADCPGHEQYTRNMATGASTCDLAIILVDARHGVMTQTRRHSFIVSLLGIKHVLIAVNKMDLVDYSEERFEEIKRDYREFATRLEMPDQHFVPISALKGENLIAHSEKMPWYEGSTLMHHLENVHIASDRNLIDFRLPVQV"
  },
  {
    "reference": "MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTVVIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISSGDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHIGRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGVVVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTTWGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLLAVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein localizes to the plasma membrane.2. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "IPAEVVLPLAGYWVSKGDMTLAGVVLAGSLGGVAGPLTLYWIGRYGGRPFLERFGKYLFIKPEALDKSDNFFKKHGGFVAFSGRFLPGIRTLISIPCGIAKMNVWVFSLYTFIAMLPITFVYVYLGA",
    "response#2": "IPAEVVLPLAGYWVSKGDMTLAGVVLAGSLGGVAGPLTLYWIGRYGGRPFLERFGKYLFIKPEALDKSDNFFKKHGGFVAFSGRFLPGIRTLISIPCGIAKMNVWVFSLYTFIAMLPITFVYVYLGN",
    "response#3": "IPAEVVLPLAGYWVSKGDMTLAGVVLAGSLGGVAGPLTLYWIGRYGGRPFLERFGKYLFIKPEALDKSDNFFKKHGGFVAFSGRFLPGIRTLISIPCGIAKMNVWVFSLYTFIAMLPITFVYVYLGN"
  },
  {
    "reference": "MTNSEYVLSKEEISGLDRRTIESGIPSRTLMRNAAYAVYRRILALGADMSRTVIVCGGGNNGGDGYALALLMEKSGIPVSVLESSAPKSDDCVYYSSLLRAARADISALASATLIVDAMYGFSFRGSLLGRDAEIAAAVNSSEAFVLAVDLPSGVSADGDFCEGCVCADATCTFTAEKYATASYPSKAKCGRVFVEDIGVPSSMLEKLSPGALLVRSADGMIKPRSDTGHKGSFGTLAVLAGCADMPGAAALCAAGALCSGVGLVKIASEEKVLDILKIKLDEPVYLRASVDAITESGASALLVGCGIGRGYDGMLADLLTASEVPAVIDADGINFLAAHINVLKEMRCPAALTPHPGEMARLTGTDVREVERNRIRTAGDFAQKYGCYLLLKGSRTVIAAPDGRIRINTTGNSALSKGGSGDVLAGVAASLAAQGYDLFDALTVAAYVHGKAGERLSALYGAHGARASRLPEEIGRLLG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.2. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.3. The protein should have a high affinity for (6S)-NADPHX.4. The designed protein must be optimized for NADHX epimerase activity, NADPHX epimerase activity. The designed protein sequence is ",
    "response#1": "VVVVAGVMQGAAFLAAEAAQRAGAGYVEVTGDDLPFGTPLSLVRRAWSDDVLADPRIGAIVIGPGLAANDAGRARLEVALATDRPMVLDAGALALLAEHGDDVAPRLRGKSTAMRLLTPHAGEFERLFGPVGDDPLGATRKAAARAGAFVLLKGATSIVAAPDGRALIGPVASPWLATAGTGDVLAGVCGAMLAQAGWHGFDALAAAGAAIWLHARAAELP",
    "response#2": "NTKKGDNGRVLIIGGNFTFSGAPTLSALGALRTGADLVYVASPEETAKVISSFSPDLISIKLKGKNISTDNLDELKPWIDKADVVVVGPGMGQERETVDASIEIVRYLKAKNKPSVIDADALKSVAGMELFPNAVITPHAGEFKIYSGVQPDSNMRKRIEQVKECSLKCNCVVLLKGYVDIIAEKEEFKLNKTGNPGMAVGGTGDTLTGIIASFMAQKLSPFTSAYLGAFVNGLAGSIAYEKLGAHLVATDIIENID",
    "response#3": "PWSHKGTFGHVAVIAGSTGKTGAAHMASLAALKVGAGLVTLIIPESLNGIMEVKLTEVMTYPVKDRGRGYLPTSAYKEIIDFVEDKDVVVMGPGLSQNRETMELVRKLYGNVQKPFVVDADGVNAFQGHPDMIRTKRHGTVFTPHPGELARVTGITPQAVNADRIGTGRSFTGKYGVHLLLKGARSILFDPGGEIIINPTGNPALAKGGSGDILTGFIGGFVAQGYSLTEATILAAYIHGYIADTWAEGGVGLDMLTGDWAKSVLPERPLGANKGSFGKVLVVAGSINYIGAAYLACMGAARVGAGLVTLSTASSLQPILAAKLTEVTYAPLPESEAGIIASKATSVLKELLPGYEVLLMGCGLGQKTQVVEFIKSALFGLTRGSSLSLVLDADVLNTLAQLPNWWQKLNQDAILTPHPGEMARLVGVSVEEVQKQRLEITRKAAMKWRKVVVLKGAYTVVAASDGRARISQVANPGLASAGTGDVLTGTIAGLAAQGLSLFDAAACGVYLHSEAGEVVRRKMGDAGMLASDLLPVLPKVIMRLKN"
  },
  {
    "reference": "MSMDNLVRLVEKDQYKQLPDFRPGDTVKVHVKIVEGGKERIQIFEGIVIKIRGSGLGKTFTVRKIASGGIGVERTFPYHSPVVQKIEIVKKAVTRRAKLYYIRDIRGKIRLKERKE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site..2. A protein that contributes to translation by functioning in ribonucleoprotein complex, ribosome with structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "PTVRGSVMNPNR",
    "response#2": "PTVRGSVMNPNR",
    "response#3": "PTVRGSVMNPNF"
  },
  {
    "reference": "MEAGLARFSHLARIVPGSRRLGGDAEIRGIACDSRRVTSGDLFVAIPGYDRDGRAYIKDAIARGATAVVSEAPIPQIGVPRLEVPDAREALALLARALHGQPDEVLTMVGITGTNGKTTTALLLAAACDAAGLSAGVVGTLGAGSGGVFTPGLHTTPEAPDLYTALDNILHAGHKAAILEVSSHALALKRVHGMKFDVAVFTNLTRDHLDFHKNMNQYFAAKARLFLGLPPHAAMAVNLDDPFGTRLISPSRTRVVTYGRATRADVLPLELTTTIDGLRLKLRTPKGDVVVESALLGRTNVANIMAAIAAACALDLNLEDAAAGIHEVKLIPGRAERIDEGQEFLVLVDYAHTDDALIQILGSARELAAGRLLVVFGCGGDRDTSKRALMGAAAARLADMAFLTSDNPRSEDPLAILAQVETGFRGQNPAVAPVIEPDRTAAIRAAVAEARPGDVLVIAGKGHETRQILADRNIPFDDREVARQALRDRLGKGA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein contains novel Mur ligase central domains that confer a unique function or activity.2. The protein should be able to bind ATP ligand in a variety of conditions3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.4. A protein that contributes to cell cycle by functioning in cytoplasm with ATP binding, magnesium ion binding. The designed protein sequence is ",
    "response#1": "VTGTNGKTTVVTLLHQLFINLGISAGLLSTIEDKINQETFPSTHTTGDSIHINKMLSKMVLAGCEFCFMEVSSHAIHQNRIKYLDFDGAVFTNISHEHLDYHKNFLNYINTKKLFFQELKSTAFSLINEDDKNAGVMISNSKSRKVTYSLKSMSEYRCKIKENTIDGMLLDINNDDVWVRQTGMFNAYNLLAVFSY",
    "response#2": "VTGSNGKTTVCALLDSILSHARLSVGVIGTTGTRYPGMQGKPGLTTPDPVTLQATLKDMLDAGCDAVALEASSHALDQRRLDGLQPVSAGFTNLSRDHLDYHGDMESYYLAKKRLFSGLHPQTACLNLGDASGRRLAAELAGGPILLGYAVDGAAQAGARILPRLAALNPQFDANGCRFTLQDAFSDATVEATLPLPGRFNLDNALCAAAMALLELGVAPR",
    "response#3": "VTGSNGKTTVCALLDSILSHARLSVGVIGTTGTRYPGMQGKPGLTTPDPVTLQATLKDMLDAGCDAVALEASSHALDQRRLDGLQPVSAGFTNLSRDHLDYHGDMESYYLAKKRLFSGLHPQTACLNLGDASGRRLAAELAGGPILLGYAVDGAAQAGARILPRLAALNPQFDANGCRFTLQDAFSDATVEATLPLPGRFNLDNALCAAAMALGL"
  },
  {
    "reference": "MKRVFRGPWLWIVVAVLAVLLALEFLAPGGGYDEVSTSTMSKYIDEGQVKEITFIDGDQTIEATLDKGTRDEGDKVMTHYIQGQQEGILEAVDEQVASGDIDKSNSENPQPSLLGSLLATLLPFALIILLFIFLMNNVQGGGRGVMQFGKSKAKMISKDMPKSTFADVAGCDEAIEELGEIKEFLQEPAKFQAVGAKIPKGVLLYGPPGTGKTLLARAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFEQAKENAPAIVFIDEIDAVGRHRGAGMGGGHDEREQTLNQLLVEMDGFDVRGGVILIAATNRPDVLDPALLRPGRFDRQIGVDAPDLSGRKRILEVHARGKPLAPDVDLMAVARRTPGFSGADLANVLNEAALLTARNNQKQIVATALDEAIDRVIAGPQRRTRLMTEKEKLITAYHEGGHALVAAALPGTDPVHKITILPRGRALGYTMVLPDEDKYSQTRSEMLDKLAYMLGGRAAEEMVFHDPTTGAGNDIEKATSVARAMVTQYGMTERLGAIKLGESNSEPFLGRDLGHQRNYSEDVAAIVDEETKLLLAAAHQEAFDILVENRDVLDALVLALLDKETLDKAEVAEIFEPLRKRPERPAWTGSPTRIPSEIPPVEIPEEIRKRAESNGGLNGSTPQEDEQEGAGAVLTPPGPGGDVYGGPPIAPSTDGPVPPSSPGS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The active site active site of the designed protein must be optimized for substrate binding and specificity.2. The protein requires Zn(2+) to activate a critical catalytic site.3. The protein should have a Helical domain that is long enough to span the entire membrane.4. The designed protein must have at least one AAA+ ATPase domain.5. The designed protein must possess ATP binding to support protein catabolic process under certain conditions. The designed protein sequence is ",
    "response#1": "LLVGPPGTGKTLLAKAVAGEANVPFFSMSGSDFVEMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAIGRARGRNPNMGSNDERENTLNQLLTEMDGFDTNSGVIILAATNRADMLDRALMRAGRFDRQIHVLFIDA",
    "response#2": "MPKGVLLVGPPGTGKTMLAKAVAGEANVPFFSMSGSEFVEMFVGMGASKVRDLFRQAKEKAPCIVFIDEIDAIGQKRSGGNFGGNDEREQTLNQLLTEMDGFDSNNGVVILAATNRPESLDPALTRPGRFDRRVPVELPS",
    "response#3": "LLIGPPGTGKTLLAKAVAGEANVPFFSISGSDFVEMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAVGRARGKNVNFGSNDERENTLNQLLTEMDGFGTNMGVIILAATNRADILDRALLRAGRFDRQIHVLFADALLGQGIEQIRAR"
  },
  {
    "reference": "MTLGKNKRISKGGKRGKKKAQETMSRKEWYDVVAPKNFEVRQFGKTICNKTQGTKIAADYLRGRVYESNLADLNKTQGDDDAYRKVKFVVQEVQGRNLLTQFHSMEMTSDRVYFLLRKWCTTIEAAVEAKTADGYTLRLFVIAFTKKQSNQLSKNCYAKTRLVKWVRHRITNLIRQRLSKVNINEAVTLLTRNILRDRLAKRCNPIVPLRDLRIRKVKVVRTPRFDAQALLNAHGEIPASAEGEARVVEEAQEAPAAEATA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must be able to structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "PKILITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSLQKGTFY",
    "response#2": "PKILITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSLQKGTFA",
    "response#3": "PKILITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSLQKGTFV"
  },
  {
    "reference": "MARRPRPPFLFFEEKPLVPFSLKTFIDRHPARSYHPFVQPVIRAAQMRLIDRLTTENFKTPSLLLMEAAANSCLRAIESHFDGKLSDKTARILCGRGNNGGDGAALARALARVGVHCDVVLFGKLSELSGDAESNMQAVSELASFAAGSAVSPSPLTFVECDSVASWENIARPRRSYDLIVDALFGTGLTRPLEGVFLKVIEHLEMLRGARERAADVHPLILSVDVPSGLNADLAQPIGATVQADLTVTFTAAKPANVLSPAAEFGGKFIVADIGSPRSLVDATKPDLFLIDAEDARSWLTKTRYTPNSYKNTHGHALVVAGARGYFGAAALCGNAAMRSGAGLVTIATPASAQSTVAANAMSEVMTTALAETDRGSVSDEAIEHLQKLSAKTTAIAIGPGLTSDDERTRKFVHNVVANRTTPVVIDADGLNCLSTWPQDLRGSDEFPLILTPHPGEMLRLMGQTDKSAIDDRVLAARSFAVAHHVIVVLKGPRQLIATPDGRVFINATGNAGLGTAGAGDTLTGLIVGFISQAQATLGSNADSLEATIAALYVGGLAGDFAASEIGMRTMVASDIREHFSHAIRFLDPDGERPPTTHT",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must have high specificity towards NADPHX epimerase activity.2. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.3. The protein contains novel YjeF N-terminal domains that confer a unique function or activity.4. The (6S)-NADPHX, K(+) binding site should be stable and able to withstand changes in environmental conditions.5. The protein should be designed to catalyze the following reaction (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "MAKYDGYAIKTWGIPSTVLMENAGRNTYRLMKEQYLAGAAAIAVVCGRGNNGGDGFVISRYALRDGFRTTVFLLGKRSELKGDAALNMELYASIGGEVVECTKDIDALRKGIQGADVLVDAIFGTGLSKATGGNEKLAIEAMNGSGKPVIAVDIPSGIDGKTGLPLGAAIRATHTFTYGYPKLGQILYPGAYHAGRLTVIDISIPASIEGRGDLLPTLQSRGKRFHKGQFGRMLVLGGSKNYTGAPSFSSLTGINFGLDLVITYTPQIIGDVIRSYSPNLIVRSQPGDWLSKQNWEEIIWLVEWSNAILIGPGMGEEKETEALLIDLLKKFREDKKAFVLDADALKLVKNHHDLLKRQSCILTPHEGELKIMTGAELPPYDDIEARSESIIKLAKELNCVLLVKGPYDYISDGEELRINRTGCQEMAVGGTGDVLAGLCASFLATGNKIFESACSAAFLNGYIGEFCKQKVGTRFNAMDMIDKINEAISELEL",
    "response#2": "MVTDLNCEYLGLSRLCLMESAGKSLAEEIGKIAVYTFSKPVKIIIFAGSGGNAGDAFVATRYLLNKGYDVDIYMLTQENKIKSNDAKSNFKILENLKPRLSHLKIYNLATVEDINSCELVNSDEFSDFIIIDAILGTGIKGKLRDKVRRAIEVINESKGLKISVDIPSGMNALTGKIEDLAVKPDYTISFHKIKTGVRISDEEKVGGLVTADIGIVLIFAGSKKYTGAAYICTEGAVRSGAGLITLCCDEDILQILKNKLAEAMTISSKDNDGIDKLLLNSNCIAIGPGMGNNENTFKIVNYVLKASNCPVVIDADGINVLSNNLGILNNVKVPVVLTPHLGEMARLTGFSIEYIEQNKMKVVKDFAITYKVIVLLKGYNTIITDGNIAIVNPTGNSSMASGGMGDCLTGIITSFIAQGQKPIIAAMTGAFLHGYCGEMLSREMFNVSATDILNKIS",
    "response#3": "MMVTDLNCEYLGLSRLCLMESAGKSLAEEIGKIAVYTFSKPVKIIIFAGSGGNAGDAFVATRYLLNKGYDVDIYMLTQENKIKSNDAKSNFKILENLKPRLSHLKIYNLATVEDINSCELVNSDEFSDFIIIDAILGTGIKGKLRDKVRRAIEVINESKGLKISVDIPSGMNALTGKIEDLAVKPDYTISFHKIKTGVRISDEEKVGGLVTADIGIPFEAELLIGGGWGMAGALGLNIIGALSCGSTYLNVSTDESCYPCLASRFLTCVYHPFNEYNWEGIIKSILKNNNSIAFGSGSVRLPAKNKILNLLLESVKTTLVLDAEAINLLSRNYYVLNLTKAKVVLTPHIKEFSRLIDTPIEVIMQNKFEIAKDFCEKHNVILVLKGVHTIVVSNKYIYINQTGNQILARAGSGDLLTGMITALTSIINDTYLATIMAVWLHGYIADIAKERYAECAKAAGATYIAGSGDVLAGVDKNKFLENYPIISENSYKGKNGRALLIGGGWGMAGALGLNIIGALSCGSTYLNVSTDESCYPCLASRFLTCVYHPFNEYNWEGIIKSILKNNNSIAFGSGSVRLPAKNKILNLLLESVKTTLVLDAEAINLLSRNYYVLNLTKAKVVLTPHIKEFSRLIDTPIEVIMQNKFEIAKDFCEKHNVILVLKGVHTIVVSNKYIYINQTGNQILARAGSGDLLTGMITALTSIINDTYLATIMAVWLHGYIADIAKERYAISNFQLENYQELLEEFFGRGDLLPTLQSRGKRFHKGQFGRMLVLGGSKNYTGAPSFSSLTGINFGLDLVITYTPQIIGDVIRSYSPNLIVRSQPGDWLSKQNWEEIIWLVEWSNAILIGPGMGEEKETEALLIDLLKKFREDKKAFVLDADALKLVKNHHDLLKRQSCILTPHEGELKIMTGAELPPYDDIEARSESIIKLAKELNCVLLVKGPYDYISDGEELRINRTGCQEMAVGGTGDVLAGLCASFLATGNKIFESACSAAFLNGYIGEFCKQKVGTRFNAMDMIDKINEAISELE"
  },
  {
    "reference": "MPAIQNIRAKEILDSRGNPTIEVTLWLDDGQAVVTSVPNGIKHYPNEAFVLYDQDSEHMGGRGVQKAVALINQTIAPQLTGQDPTQQTPLDQFLQQLDSTPQKSKAGANTLIALSQAVLKAGAVATGQPLYQYLIQQFQLTDHAAIPTCLYGLVNGGQYGSDNLDIQEFLVIPASHLDYRVSLEMESALRTKLQEMLKNQKANYSFGELGGFTPALQKNTDVFEFIVEAARKTNYILSRDFFFGIDTGGDNLVSGGKYLLKDKSTGYSEKELLEYYKNLREKYNLTYFEDPFAVKDAGAWQAITTDLADAARIAGDVITATQPSLVQHAIENRLCNTLVVKPSQVGTVTETLQAIKLARNAGWAIVISQRTGETNDDFLADFAVGVGAEFVKFGPTNRGEAVAKYNRLADIYQALEQTHQEGTAMTEPTQPTNTSTPAVPPPDEPTVDQTTEPTSVLGTPPAPPAPAADPSPTDLPQNTSIPVVEPPQPPTSTPAKPPVVSATPMPPKPPAGPAVTPPATPPPNIPPPSQPPTPPAPPTTPLAAAGPTEKPSEADVQQSIDKTLSEIGAKPPAVPGGETTAPPSAPAKPASPTASEPTPQE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein localizes to the cell surface.2. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.3. The protein should have a high affinity for Mg(2+).4. The protein contains novel Enolase C-terminal TIM barrel domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "EVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAASFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFATPSGKPATLTRWSLAWTWPLLSSSGRASTTWTSSPPTTPAGTSRRTSWPACTSPSSRTTQVSARWPRAGGRPN",
    "response#2": "EVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAASFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFATPSGKPATLTRWSLAWTWPLLSSSGRASTTWTSSPPTTPAGTSRRTSWPACTSPSSRTTQVSARWPRAGGRPL",
    "response#3": "EVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAASFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFATPSGKPATLTRWSLAWTWPLLSSSGRASTTWTSSPPTTPAGTSRRTSWPACTSPSSRTTQVSARWPRAGGRPN"
  },
  {
    "reference": "MTLERTLSIIKPDAVGNNHIGEIIARFEKAGLRIVAAKMKQLDRKDAEGFYAVHKGRPFFEDLVGFMTTGPVMIMVLEGENAINKNREIMGATDPKKAAPGTIRADFAQTIDENAVHGSDAAETAKTEIEYFFKSQDVCARKR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Major role in the synthesis of nucleoside triphosphates other than ATP; The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.2. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining nucleoside diphosphate kinase activity, ATP binding. The designed protein sequence is ",
    "response#1": "MEKTFAIIKPDAFAAGNAGKILARIYQEGFTVIGLKKLCMSQREAEGFYAVHNKKPFFAELTKFMSSGPCIVMVLEAEGCIGKWRDLMGATNPADAKPGSLRREFGTIVGENATHGSDAPETAAVELEYFFSGLELV",
    "response#2": "MEKTFAIIKPDAFAAGNAGKILARIYQEGFTVIGLKKLCMSQREAEGFYAVHNKKPFFAELTKFMSSGPCIVMVLEAEGCIGKWRDLMGATNPADAKPGSLRREFGTIVGENATHGSDAPETAAVELEYFFSGLELK",
    "response#3": "MEKTFAIIKPDAFAAGNAGKILARIYQEGFTVIGLKKLCMSQREAEGFYAVHNKKPFFAELTKFMSSGPCIVMVLEAEGCIGKWRDLMGATNPADAKPGSLRREFGTIVGENATHGSDAPETAAVELEYFFSGLELI"
  },
  {
    "reference": "MAKTAIAYKEKMKELSMLSLICSCFYPEPRNIAAYTFDDMEVKQLNKRASGQAFELILKPPSPVSEAPRTLASPKRKDVSLEEIQAKLEAAEERRKSQEAQILKQLAEKREHEREVLQKALEENNNFSRMAEEKLVLKMEQIKENREYYLASLMERLKEKERHAADVRKNKEQLELSG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be optimized for stability and solubility when expressed in neuronal cell body where it will be involved in cellular response to nerve growth factor stimulus, negative regulation of microtubule polymerization.2. The protein contains novel SLD domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "SSDWQMKEKGLVSIQRLAACHSEVLTGKLHDVCLVVTGEVTNLRSKVSHLAISTLGDLFQALKKNMDQEAEEIARCLLQKMADTNEFIQRAAGQSLRAMVENVTLARSLVVLTSAGVYHRNPLIRKYAAEHLSAVLEQIGAEKLLSGTRDSTDMLVHNLVRLAQDSNQDTRFYGRKMVNIC",
    "response#2": "SSDWQMKEKGLVSIQRLAACHSEVLTGKLHDVCLVVTGEVTNLRSKVSHLAISTLGDLFQALKKNMDQEAEEIARCLLQKMADTNEFIQRAAGQSLRAMVENVTLARSLVVLTSAGVYHRNPLIRKYAAEHLSAVLEQIGAEKLLSGTRDSTDMLVHNLVRLAQDSNQDTRFYGRKMVNICV",
    "response#3": "ANRRAEVIDWLGGLLPEFDLPLDSSDEELRDYLINGEALCYVADKLMPGVLEGTWGGYASDQRSNVKKFLSVVAEMGLPGFGVKDLEEGSMSSIVECLLALKDNVL"
  },
  {
    "reference": "MGLHVYLLLLFSPLAGAILDITLIANVQSLSHSDFFLSCVVGERDVSDLQIERENKIVMTHPKMGFQNYRNRSNLVQARGFSKADLVGILYCLGRTPTEQAQVVYVHNSHNAHLFPVKATQSVNVAEAATFSARILKRKETDVMWKRNGTYYQTTDRGEVQDDHVVLTLPSVSVSENGVYSATFMGDSPLWSAFYRLIVRACPAKKWGPSCEKDCPDCLNGGICHDHVGECICPPGFMGTRCERACQEGQFGRNCQETCQRAQGCRGLSFCLLDPYGCSCASGWSGSRCDQACPSGFYGPDCALECTCQNGGSCNRFSGCVCPAGWHGQHCEKSDRFPQIIQLASELEFNLGSEPIISCVAIGNPLPTRDSVELRKADGTVLKVIKTIIEPKQITCEFEVRHLTKADTGLWECRVSTTGGQDSRKVKVNIRVPPVPLSAPRLLAKQSRQLVVSPVDNFSGDGPITSIKLFYKPKDDNSAWSSIVVDNSENITLMNLRPVTAYIVKVQLSRPGAGGEGSKGPEAIMVTDCLEPTVKPVIEGWSIEEKNMLHVNWKLPSNHEPAHGFIVHLFDSARRLVSEKNITSISVLSARIGDLEFNKEYRLEVLVYHCTSLGPPSDPYKVMINSKGPSSPQLLSAEPVSDTAVRLSWQVPEYPNGGITKYIVELQQVGGTSEPQWIDTDSGAETTKIVGGLNASTNYQFRVRANSHVPGEWSQPVKAKTLGDGVLSVPPSLGSQSTEQAGTDQQLLLAIVGSVSVTCFTILFALLA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "VLFWLCILSFFSVLNEMVLNVSLPDIANHFNTTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNHFFILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGGIIAHYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYNWTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGGLIFSIVAGFISMVPYMMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVFILGSLSISISFLTIAFFVEFSMWLTTFMFIFVMGGLSFTKTVISKIVSSSLSEEEVASGMSLLNFTSFLSEGTGIAIVGGLLSLQLINRKLVLEFINYSSGVYSNILVAMAILIILCCLLTIIVFKRSR",
    "response#2": "VLFWLCILSFFSVLNEMVLNVSLPDIANHFNTTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNHFFILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGGIIAHYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYNWTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGGLIFSIVAGFISMVPYMMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVFILGSLSISISFLTIAFFVEFSMWLTTFMFIFVMGGLSFTKTVISKIVSSSLSEEEVASGMSLLNFTSFLSEGTGIAIVGGLLSLQLINRKLVLEFINYSSGVYSNILVAMAILIILCCLLTIIVFKRSE",
    "response#3": "VLFWLCILSFFSVLNEMVLNVSLPDIANHFNTTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNHFFILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGGIIAHYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYNWTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGGLIFSIVAGFISMVPYMMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVFILGSLSISISFLTIAFFVEFSMWLTTFMFIFVMGGLSFTKTVISKIVSSSLSEEEVASGMSLLNFTSFLSEGTGIAIVGGLLSLQLINRKLVLEFINYSSGVYSNILVAMAILIILCCLLTIIVFKRSQ"
  },
  {
    "reference": "MSNSSIYGLTYEQLKLWLMEHGEKRFRADQVWNWLYKKRISRFEDMNNVNQSTIDLLKENFVLHTMGEEIRQESKDGTIKFLFKLADGNVIETVLMRFNYGLSVCVTTQVGCNIGCSFCASGLLRKNRDLTSGEIVEQIMNVQKHLDERGEEDRVSHIVVMGIGEPLDNYKNLMDFLYIVNDDGGLNIGARHITVSTSGLAHKMYEFADEPIQVNLAISLHAPNNELRTKIMKINRAFPIEKLMKAVDYYLEKKNRRITYEYIMLHDVNDHKKEARELAKLISGHRHLAYVNLIPYNTVDEHIAYQRSSSENIQAFYETLKAEGIQCGVRWENGADIDAACGQLRSKQIKKEKAV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must bind to [4Fe-4S] cluster in order to perform its enzymatic function.2. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.3. The designed protein should have 4 iron, 4 sulfur cluster binding to facilitate rRNA base methylation. The designed protein sequence is ",
    "response#1": "APGRATVCISTQVGCQVRCVFCSSGSEGLIRNLTAAEITDQVLLSCRHLGAKINNIVVMGMGEPLHNLDNLTRALDHLNAPDKFALGARHITISTSGIVPGIRQLADLHRPWNLAISLHATSDQQRAQIIPPACRYPIGEIISACQYHRQQTNRMPTLEYALLKDFNDSPEDAVRLAALAARLYAKINLIPCNPGPSRYQAPAPAKCRAFLERLIRAGAQATLAR",
    "response#2": "EPSRGTLCVSSQVGCTLTCSFCHTGTQRLVRNLSAGEIVGQVLMARELLGDFPGGTRPVNGLVPRGETRAITNIVMMGMGEPLYNFENVKNAMLIFSDGDGLSISKRRITLSTSGVVPQIEATGKETGVMLAISLHAVNDELRNKLVPINKKWPIKELLLACKNYPGVSNARRITFEYVMLKGVNDSDADARELVRLLANIPAKINLIPFNPWPGSPYECSDWETIERFSDIVNKAGYASPVL",
    "response#3": "SSQAGCGMNCPFCATGQAGLTRNLSTGEIVEQVAGGQRALNRGDIAGGPGRVSNIVFMGMGEPLANYKAVLGAVRRITEPSPSGLGISQRGVTVSTVGLVPAIHKLTEEGLSVRLAVSLHAPDDELRDTLVPINTRFKVREVLDAAFGYARKSGRRVSIEYALIKDVNDHAWITP"
  },
  {
    "reference": "MAKYIFVTGGVVSSIGKGIVAASLGRLLKSRDYSIAILKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTDTSMSKLSNVTTGAIYQGVINKERRGDYQGGTVQVIPHITNEIKERIHRVAKNGNPDLVIVEIGGTVGDIESLPFIEAIRQFRKDVGRQNVVYMHVTLMPWIASAGEMKTKPTQHSVKELQSVGIQPDILVCRSDRPLPQNIKDKVSEFCNVLPECVMTGQDVKSIYEVPLAMEREGLAEQVLRLLGMQQRQPDLRSWQTLVERLYRSERNVEVAIVGKYVRLTDAYLSVIEALKHGAIAVNSNVNLRWINSEDIEAYGADKFLKDVHAIVVPGGFGHRGVDGKVAAVQYARDRQIPFLGLCLGMQCAVIDWARNVGQLSDANSAEFAPESKNPVIHLLPEQQDVVNLGGTMRLGLYACRLAPNTLVSELYNESVIYERHRHRYEFNNAYRSLFLESGYVISGTSPDGRLVEVVELPSHPYFIATQFHPEFQSRPNSPHPLFQGLIKAALDVQSDRLANGSESAIAPIIEQLNEQQQEFASATSA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile, active site, Nucleophile; for glutamine hydrolysis to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).2. The protein should be optimized for activity in Pyrimidine metabolism, CTP biosynthesis via de novo pathway, CTP from UDP: step 2/2 pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The protein should be able to bind CTP, L-glutamine, UTP, ATP ligand in a variety of conditions4. A protein with CTP synthase activity, glutaminase activity is needed to enhance 'de novo' CTP biosynthetic process in a specific context.5. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen6. The designed protein should contain one or more CTP synthase N-terminal, Glutamine amidotransferase domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MFVTGGVCSSLGKGIATASIGAIMKAAGLKVFVQKLDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDEPMSRLSTVTTGRIYSDVIAKERRGDFLGGTIQVVPHITNAIKHSIISAAEQSGTEIMMVEIGGTVGDIEGEPFLEAVRQMRGELGPQRIFHVHLTLLPYLKGSKELKTKPTQLSVRELRRIGLQPDMILARADYRVPKDLLEKISRFCGVPSEAVIPALTVNSIYDVPLNFQKYDVARIIGEKLAVTLPKPKLRRVSEHTDGTRKYLFELPDGGEVETVLMRYGDPRSPAGRXXVLQDSYKSLTEALLHGGIANKTRVKIRWFDAQDLEENNTIAPHLSSVHAILVPGGFGERGSEGKIRAIQYARENKVPFLGICFGMQMAVIEAARNLANLPTASSSEFGPTETPVVGLMTEWAYGSELMRRSEGGDLGGTMRLGAYPANLKPDSQIAAIYQATKIRERHRHRYEVNVHYRKQLEECGLVMSGMSPDDVLPETIEYKNHPWFIGVQYHPELLSKPFAPHPLFSSFISARAVAGFLLHDAYKSVYEALFHAGLAGQVAVELVKIDSSKLEEAASADSILGPEAAGGGVDGVLVPGGFGQRGINGMVKAAEWARVNKVPYLGICLGMQIMVIDWARGVLGWEDGDSSEFNPDTKHPVVSLLEEQVDVKNYGGTMRLGKSESAAEPGTRIFAAYGKAQIFERHRHRYEFSNKYRTEMAGSGLRISALTPDGSLVEAVEWPDHPWGVGVQFHPEFKSKPTAANPLFRDFIEALRNGTRPIALPDAYKSVNEALKHAGLKNRLSIKIQYVDSQDLEVKGTDVLQHLDAILVPGGFGERGVEGKILAAQYARENKVPYLGICLGMQVALIEYARNVAGLKGAHSSEFDAKSKYPVVGLITEWIDEDGKVETRDVDSDLGGTMRLGSQLCHLTKGSKVADLYGSVEIYERHRHRYEVNNTILPKITAKGLQVTGLSSDKKLVEIIENPAHPWFVAAQFHPEFTSTPRDGHPLFEGFV",
    "response#2": "MFVTGGVCSSLGKGIATASIGAIMKAAGLKVFVQKLDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDEPMSRLSTVTTGRIYSDVIAKERRGDFLGGTIQVVPHITNAIKHSIISAAEQSGTEIMMVEIGGTVGDIEGEPFLEAVRQMRGELGPQRIFHVHLTLLPYLKGSKELKTKPTQLSVRELRRIGLQPDMILARADYRVPKDLLEKISRFCGVPSEAVIPALTVNSIYDVPLNFQKYDVARIIGEKLLPDAYKSINEAFVHAGAKNECKVRLKHIHSEGLTPENAAEKLAGMHGVLVAPGFGSRGIEGKIEAIRYVRENNIPFFGICLGMQCAVIEYARNVIGWKDAHSTEMDDHTKHPVISMMEEQKKIKNKGGTMRLGAYACDLKKGSKAASIYGKAHISERHRHRYEFNSKYLKEFEDAGLIASGINPENNLVEIVELKNHPFFIGGQFHPELKSTVANPHPLFVNFVSAAANAALEDAYLSVLEALKSAAVHQGRKAEIVWIDSEKLELKDKEVWAELERCKGIVVPGGFGIRGIEGKIAAARYARTKKIPYLGLCLGSQILAIEFARAKLKDPQITSEEFDEEGKIPKSKYLVHFLPGQHKGRAKGGTLRLGAYTCQLREGTKASAAYGQEEISERHRHRYEFNNAFRRKLEEKGLVFSGEWTETGLMEIVELKGHPFMLGSQFHPEFKSRPHRPHPLFRAFLSRIGAA",
    "response#3": "MFVTGGVCSSLGKGIATASIGAIMKAAGLKVFVQKLDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDEPMSRLSTVTTGRIYSDVIAKERRGDFLGGTIQVVPHITNAIKHSIISAAEQSGTEIMMVEIGGTVGDIEGEPFLEAVRQMRGELGPQRIFHVHLTLLPYLKGSKELKTKPTQLSVRELRRIGLQPDMILARADYRVPKDLLEKISRFCGVPSEAVIPALTVNSIYDVPLNFQKYDVARIIGEKLLQDSYKSLTEALLHGGIANKTRVKIRWFDAQDLEENNTIAPHLSSVHAILVPGGFGERGSEGKIRAIQYARENKVPFLGICFGMQMAVIEAARNLANLPTASSSEFGPTETPVVGLMTEWAYGSELMRRSEGGDLGGTMRLGAYPANLKPDSQIAAIYQATKIRERHRHRYEVNVHYRKQLEECGLVMSGMSPDDVLPETIEYKNHPWFIGVQYHPELLSKPFAPHPLFSSFIAAVKKVRQREAKLQDAYKSIYESLTHAGASHDTRVVIQRVEAEAIEKDGAAAHLDGMNAILIPGGFGDRGTEGKILTAKYSRENNIPFFGICLGMQIATIEFARNVCGLENANSTEFDKNTPHPVISLQEEQQGVEDLGATMRLGASDSQVLAGTKAAELYGSDSISERHRHRYEFNPAYREQIEKAGLKISAVSDPEGLVEIVEIADHPFFIGAQFHPEFQSKPNAPHPIFSGFVRAQAIREQS"
  },
  {
    "reference": "MTLDPNVAARLKRNADGLFTAVVQERGSGDVLMVAWMDDDALDRTLKTREATYYSRSRGEQWVKGATSGHTQYVHSVRLDCDGDTVLLTVDQVGGACHTGDHTCFDATVLL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be optimized for activity in L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9, L-histidine biosynthesis, Amino-acid biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The protein must bind to Zn(2+) in order to perform its enzymatic function.3. For general function, the protein need meet that Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.4. The designed protein should have phosphoribosyl-AMP cyclohydrolase activity, zinc ion binding within cytoplasm to regulate histidine biosynthetic process.5. The Zn(2+), Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.6. The protein should be designed to catalyze the following reaction 1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-   D-ribosyl)-5-[(5-phospho-beta-D-   ribosylamino)methylideneamino]imidazole-4-carboxamide. The designed protein sequence is ",
    "response#1": "MMAWMNAEAVARTLETGRVTYWSRSRQSFWVKGETSGHIQRLVELRIDCDRDCLLLLIEQVGPACHTHRRSCFLARARDIPEFVADGVADIGVTGYDLVLERNVEDKVDFLLDFGFGFAKLVLAAPESSNINSIDDIKEGMRVATEFPNLTKKYFEKLNKKVEIIELSGATEIAPFIGIADLISDLTSTGTTLRLNRLKVIDEIVSSTTRLIANKNSLKDKEKREKINQIVIAIAETKRLIMMNAPKDKVEEIRKLIPGMAGPTVSKVLSDDNMVAIHAVVNEDEIFTLVPKLHALGARDILVVPIETAETKRLIMMNAPKDKVEEIRKLIPGMAGPTVSKVLSDDNMVAIHAVVNEDEIFTLVPKLHALGARDILVVPIET",
    "response#2": "MLAYMNQEALNLTLSTQVAHYFSRSKGRIWKKGESSGHIQKIHEIFLDCDSDTILLQVEQVGVACHTGRKSCFAIRARDIPEFVADGVADIGVTGYDLVLERNVEDKVDFLLDFGFGFAKLVLAAPESSNINSIDDIKEGMRVATEFPNLTKKYFEKLNKKVEIIELSGATEIAPFIGIADLISDLTSTGTTLRLNRLKVIDEIVSSTTRLIANKNSLKDKEKREKINQIVIAIAETKRLIMMNAPKDKVEEIRKLIPGMAGPTVSKVLSDDNMVAIHAVVNEDEIFTLVPKLHALGARDILVVPIELAETKRLIMMNAPKDKVEEIRKLIPGMAGPTVSKVLSDDNMVAIHAVVNEDEIFTLVPKLHALGARDILVVPIER",
    "response#3": "MMAWMNAEAVARTLETGRVTYWSRSRQSFWVKGETSGHIQRLVELRIDCDRDCLLLLIEQVGPACHTHRRSCFLYARDSASDIASQLARGTVHLGVTGEDLVREEITDADKRVLLIDTLGFGGANVVVAVPQAWIDVRTMADLDDVASGFRAQHNRRMRVATKYINLTRGFFASHGIVDYRIVESAGATEGAPAVGTAELIVDITSTGSTLVANGLKVLDDGVILRSQANLVASRDADWSEGPY"
  },
  {
    "reference": "MTLVSDSKAPSIALVGATGAVGHTLIELIETRGLRYKGVRLAASARSAGRQLEVDGRTCTVEDLDGFDFSDVDIVFFSAGTSVSQKWARPAAAQGALVIDNTNAFRMDPDTPLVVPQVNADVLTGRPASGIIANPNCSTIPLVRLLQTVEQRWGIRQVVVSTYQAASGQGQHGIEELQEGSELALHDPDAELPAERFTAPLAFNVVPFIDRLLDSGFTMEEQKMLQESRKILGLPHLDVTTTCVRVPVVNSHSEAVWVEAAAPVDRTELIGRLAALPEVTVHDQDTPGRFPTPLTAGNPDHVHVGRVRVAPHNPRGFWLWLVADNLRIGAALNAVQIAETVLDRGLL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The substrate binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MVDVGDKEETTRTAIAEGFVSMQPETLKTILAGNAKKGDVLGTARIAGIMAAKKTHELIPLCHPLLLTKISVDIEPDHALPGLKVTALARVTGKTGVEMEALTAASVACLTIYDMAKAVDRAMLISGIRLVEKTGG",
    "response#2": "MVDVGDKEETTRTAIAEGFVSMQPETLKTILAGNAKKGDVLGTARIAGIMAAKKTHELIPLCHPLLLTKISVDIEPDHALPGLKVTALARVTGKTGVEMEALTAASVACLTIYDMAKAVDRAMLISGIRLVEKTGG",
    "response#3": "MVDVGDKEETTRTAIAEGFVSMQPETLKTILAGNAKKGDVLGTARIAGIMAAKKTHELIPLCHPLLLTKISVDIEPDHALPGLKVTALARVTGKTGVEMEALTAASVACLTIYDMAKAVDRAMLISGIRLVEKTGG"
  },
  {
    "reference": "MNKNCISASWRCDERTARQVCSADDSDKMADCCKRPEDFQCPINKTAKLDFKELRYATKVPQTPVDTVFPETTANRVEIDAKTIQLLERLSLVNLDSEQALATLNSSIQFADKIAHINTENVRPLYTVLENQQLQLRNDEVKEGDCRVELLRNAKVTDEDYYVSPPGNIPLEQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. We are looking for a protein with broad substrate specificity for ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) +   L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate.2. For general function, the protein need meet that Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria3. The protein should be engineered to have a higher affinity for a specific molecule or receptor in mitochondrion, glutamyl-tRNA(Gln) amidotransferase complex that is critical for mitochondrial translation, glutaminyl-tRNAGln biosynthesis via transamidation, regulation of translational fidelity. The designed protein sequence is ",
    "response#1": "VIGLEVHAQIQTNSKLFSGAEVAFSSPINSCVSLFDASIPGTLPVLNRKCVELGVRTALALGCKVNSVSMFDRKHYFYADLPAGYQITQQRAPLARGGLLSFPVFVPGVSRKPYYKSARLHQLQLEQDSGKSLHDPVERKSLVDLNRAGVPLMELVFEPDLETGEEAAALVKELILILTRLGSCSCRMEEGALRVDANISVHKENTPLGTRTEVKNIGSVRAVAQAIEFEIERQIGILDGGGKIFNETRAWDAAGRQTIAMRDKEVVQDYRFMPEPNLPPLHVEGAVEAAKRDMPEGRVAGNWVLHELGGLLSKAGLAWDAERVTAESLAALIDQLQRKRITGATAKKVLAMLFDGDRRPVAQLLEEENLILRPLSREEYIALASAAIELNPQMVEQIRSKNQLGKLGWFVGQMMRMGEKGRVEAQKADAILRELLA",
    "response#2": "VIGLEVHAQIQSESKLFSGAEVSFGAPINACVALVDASIPGTLPVLNRRCVELGVRTALALGCEVAPVSAFDRKHYFYADLPAGYQITQQRAPLARGGLLTFPVFVPGVTKKPYYKSARVHQLQLEQDSGKSLHDPVERKSLVDLNRAGVPLMELVFEPDLETGEEAAALVKELILILTRLGSCSCRMEEGALRVDANISVHRENTPLGTRTEVKNIGSVRAVAQAIEYEIARQIDVLDGGGRIFNETRNWDAVERKTIAMRDKEVVQDYRFMPEPNLPPLHVKKQNEEFVRKLPANWIIHELLGNLNKMEIPLIKVSHILPPEKFAELLLLISKKTISNASGKLLLSHILNSFKENSFVASEPINFNALIEEFELHAVSDVGSEELELICESIITELNNNKMIEDIKSGKKKNSIKFLVGQAMRISQGRIQAQEFEITFKRL",
    "response#3": "VIGLEVHAQIQTNSKLFSGAEVAFSSPINSCVSLFDASIPGTLPVLNRKCVELGVRTALALGCKVNSVSMFDRKHYFYADLPAGYQITQQRAPLARGGLLSFPVFVPGVSRKPYYKSARLHQLQLEQDSGKSLHDPVERKSLVDLNRAGVPLMELVFEPDLETGEEAAALVKELILILTRLGSCSCRMEEGALRVDANISVHKENTPLGTRTEVKNIGSVRAVAQAIEFEIERQIGILDGGGKIFNETRAWDAAGRQTIAMRDKEVVQDYRFMPEPNLPPLHVEYYQDVVDILRNLQQDLDSKTQAGLGRVAGNWVLHELGGLLSKAGLAWDAERVTAESLAALIDQLQRKRITGATAKKVLAMLFDGDRRPVAQLLEEENLILRPLSREEYIALASAAIELNPQMVEQIRSKNQLGKLGWFVGQMMRMGEKGRVEAQKADAILRELLK"
  },
  {
    "reference": "MEGNNSSSKSTTNPALDPDLDSPDQPGLEFAQFAAGCFWGVELAFQRVGGVVKTEVGYSQGNVHDPNYKLICSGTTEHAEAIRIQFDPNVCPYSNLLSLFWSRHDPTTLNRQGNDVGKQYRSGIYYYNDAQAQLARESLEAKQKEFMDKKIVTEILPAKRFYRAEEYHQQYLEKGGGRGCKQSAAKGCNDPIRCYG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Has an important function as a repair enzyme for proteins that have been inactivated by oxidation2. The designed protein should be involved in cellular response to oxidative stress by localizing to cytoplasm and exhibiting L-methionine-(S)-S-oxide reductase activity.3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.4. The protein have enhanced [thioredoxin]-disulfide + H2O + L-methionine = [thioredoxin]-   dithiol + L-methionine (S)-S-oxide. The designed protein sequence is ",
    "response#1": "MQQAVFGLGCFWGAERRFWQQPGVFSTAVGYAGGFTPNPTYEEVCSGLTGHTEVVLVVFDPQQTSFDALLKVFWEAHNPTQGMRQGNDQGTQYRSAIYCQDHAQLEAALASQARFQAELDKAGLGSITTEICEAPIFYYAETYHQQYLAKNPGGYCGLGGTG",
    "response#2": "MPKAVFGGGCFWCLDAVFRKVDGVRDVVVGYAGGRRPNPNYEQVCTGVTGHAEVVEIDYDENTVSYDELLDIFFKIHDPTQLNRQGNDVGTQYRSIILYLDEEQKEKALKKIEELKKEGLNVVTEVKPLEIFYPAEKYHQNYFAKNPHQPYCMYVVP",
    "response#3": "MPKAVFGGGCFWCLDAVFRKVDGVRDVVVGYAGGRRPNPNYEQVCTGVTGHAEVVEIDYDENTVSYDELLDIFFKIHDPTQLNRQGNDVGTQYRSIILYLDEEQKEKALKKIEELKKEGLNVVTEVKPLEIFYPAEKYHQNYFAKNPHQPYCMYVVA"
  },
  {
    "reference": "MLRWTTAGESHGQALIATVEHMPAGVPVTKDEVSYQLARRRLGYGRGARMKFEQDALTFLTGIRHGLTLGSPISIMIGNTEWDKWTTIMSSDALDMEDPDNVAAMSSGRGAKLTRPRPGHADYAGMLKYGFDDARNVLERSSARETAARVAAATVARSFLRETLGVEVLSHVISIGASEPYVGAEPTFADIQAIDDSPVRAFGKDAEKSMIAEIEAAKKAGDTLGGIVEVIVEGLPIGLGSHISGEDRLDAQIAAALMGIQAIKGVEIGDGFEEARRRGSEAHDEVFLDDNGVYRNTNRAGGLEGGMTNGETLRVRAGMKPISTVPRALKTIDMENGKAATGIHQRSDVCAVPAAGVVAEAMVTLVLARAVLQKFGGDSLSETKSNIDTYLKNIEERMKFEGLEDGA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The NADP(+), FMN binding site should be stable and able to withstand changes in environmental conditions.2. For general function, the protein need meet that Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis3. A protein that can modulate the overall flux of metabolites through the Metabolic intermediate biosynthesis pathway, enhancing or suppressing its activity as needed.4. The protein design must be able to perform chorismate synthase activity in order to contribute to chorismate biosynthetic process. The designed protein sequence is ",
    "response#1": "MNGLEYKDSGVDIAAGNALVDRIKPLAAATERPGVLGGLGGFGALFKMSSQRYREPVVGVGSGNRLALMDLFEANGKRFDEIIGVGGRSSARETTMRVAAAVIAEKRGKKALALQGPGCGKCGIPGDSELPAGPIAHWCGGYRLRAVNQNGMEFMRHDPSIVPRAVPVAEAIAEAC",
    "response#2": "MXDXIXDXKXDXXKXDXXKXGXXDXTXDXKXGXWXW",
    "response#3": "MTQSPKPQAAILVAGGSGTRMGTSTPKQFLPLCGKPVLRWTLERFIHHPACTRIVLVIRPDDVDTTRALVTDLPAHVASRIVPVVAGGDSRQASVAAGLEALAADPPDFRHDPCVGIRATPIAEARPFVSAHQIDRVIEAVKGGDAAIVPALPVADTLKRREGASLVETVPRSDLFSAQTPQGFPFAAILAAHRRAKGEGFTHTDDAAVAEWAGLPVHVVEGSENNVKLTHRRDLMKAGAMLSPMSAHAREAPRIVNGDASLFGESHGPALTGIVEGLVSLSSERTPVADGSAGMHVRHDPCVLPRAVPMVESNGRSDPTAVPAAAVVMEGVTIGERCRLDNLIYLDGAQVGNDCNIGCGTITANYDGRKKHSVTIGNHVRIGASTTLVAPVTIGDDAVTGAGCVITKDVPAGSVVSGVPARIKSERV"
  },
  {
    "reference": "MIKSIYKNLCPNCGGEITSERLIANLPCKKCEKESGEIKKIKELQEKLKEWEKHFEKHIKGKPWGLQTTWAKRIFTFSSFALLAPTGIGKTSFGLSIASFLLKFGQKSYIIVPTRLLVEQVYEKLKNFGVNEKDLLAFDGGLKKKKKERLQKGEFKILITTSMFLYKNQSIIPKEFSFIFIDDVDSFLKTAKNIDKVLYLLGFDEESIKEAINFIKLKGKKQKSEEDLKRIEEISSQLRKIKENLKGVLVVSSATANPRSSRVKLFRELLGFEVGVPTFYLRNIVDVYTEESSLIDWIKKLGKGGLLFLPSDKGKESIEEIIEILKANNIKALSYEEINEENLELYRKGEIDILVGIASFRNPLARGFDMPDVCRYALFYGVPKIIISLKFETNLSHLLWALSSIRPFIVKNLGEYTQKINHWLLQLKKYQNLNEEFINQRENLKKKIEQLREEIAQFLTKKEIIEFLKTSEEVTLRPTEEGYLMVVSDVTGYLQASGRVSRMYAGGITKGLSLIIIDDKIAFNHLKRKIRWFSDEIDFLHISEIDLPKVLDEINADREKIKNFLIERKIGETKEILKPILIIVESPNKARTIANFFGKPIRRRIGEYDIYEISLEDRYLMITASFGHILDLTTNEGYYGVILNGEIIPIYEPIEGKEEIIKTLRKAALEVEQVLIATDPDTEGEKIGWDIKEILKPYIDDIKRMEFHEITKKAIIKAIKEPRDFDLNLIKAQILRRISDRWIGFEFSQFLHRAFGKPTLSAGRVQTP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design must be able to perform helicase activity, zinc ion binding, hydrolase activity in order to contribute to DNA topological change, DNA unwinding involved in DNA replication.2. The protein must contain a signal peptide for proper functionality.3. The protein must exhibit the following characteristics: Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process; It cleaves transiently a single DNA strand and remains covalently bound to the 5' DNA end through a tyrosine residue.4. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding. The designed protein sequence is ",
    "response#1": "MGRSFSIVAPTGIGKTHFGLIMALYLARKGKKSYIMVPTSLLVKHLLDRASFFLEKLAARGEPKPTVIGYYSGMRKKEMEETLERIRNKDFSILITTDRFLYNRFELIKDVNFDFIFVDDVDSFLKSPKNIDKVVLLMGFSIDDIERALKEEEKKEPIKAVPVASVRFNPGRVLVVSGATLRGKRTKRIKIFRRLFGFEPGV",
    "response#2": "MGRSFSIVAPTGIGKTHFGLIMALYLARKGKKSYIMVPTSLLVKHLLDRASFFLEKLAARGEPKPTVIGYYSGMRKKEMEETLERIRNKDFSILITTDRFLYNRFELIKDVNFDFIFVDDVDSFLKSPKNIDKVVLLMGFSIDDIERALKEEEKKEPIKAVPVASVRFNPGRVLVVSGATLRGKRTKRIKIFRRLFGFEPGIGRTEY",
    "response#3": "MGRSFSIVAPTGIGKTHFGLIMALYLARKGKKSYIMVPTSLLVKHLLDRASFFLEKLAARGEPKPTVIGYYSGMRKKEMEETLERIRNKDFSILITTDRFLYNRFELIKDVNFDFIFVDDVDSFLKSPKNIDKVVLLMGFSIDDIERALKEEEKKEPIKAVPVASVRFNPGRVLVVSGATLRGKRTKRIKIFRRLFGFEPGR"
  },
  {
    "reference": "MKLNELVKGLDIEYIKGNAEREIEDIVVDSRLASKGSMFVCIKGYVQDGHAFAQQAVNNGCGVLLVTDDVDVTGDVSIVKTKDTRKSLPYLADRLYNHPSGRLKLIGVTGTKGKTSTTFYIKSVLEKAKYKTGLIGTMYNMIDDEILYTERTTPEANDLQRLLNEMANREINVCVMEVSSQGLKLGRVEFCTYSTAIFTNLSRDHIGETEHPDMEDYAKSKAMLFGMSENSLINADSEYFDLMASNGKKVYSYGIEKDCDYRAVNIKKYADHVEYDVLYGTKEGKTDKRHVYVSSPGTFSVYNSLAAIGTCHIEGIDMDTIVEGIRDVTVRGKAEVVPTNRDFTVIIDYAHNPDSFINILTTVKEFAKRCVFLFGAGGDRNRPRKLMGETAGKYADFTIITSDNPRTEDPEKIVKEIEEGIKPTGAEYICIVDRKEAIEYALRNALPGDVIILAGKGHETYQIFRDKVIHFDEREVVRDILARMDEEDKRCS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have magnesium ion binding, ATP binding, tetrahydrofolylpolyglutamate synthase activity, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity to facilitate cell wall organization, cell division, peptidoglycan biosynthetic process.2. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.3. The designed protein should interact with enzymes and substrates within the peptidoglycan biosynthesis pathway to enhance or regulate its activity.4. The protein should be able to bind ATP, meso-2,6-diaminoheptanedioate, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate ligand in a variety of conditions5. The presence of Mur ligase C-terminal, Mur ligase N-terminal catalytic, Mur ligase central domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MKLAELLQDLPGAWIQAGAGPEGEITGVTADSRKAAKGFLFAALPGTKVDGRAFAAKAIEAGAAAVIADTRCAWPGGTPPCAVPGLAAARLAGRLAAGRYQPFPRQSIAIGITGTNGKTTSAFIIKNILQSNGLRTGVIGTIANWVGDQRLPASHTTPESLTLQRLLAEMVAAGCSAVVMEVSSHALAQHRVDGCRFDVAVFTNFTQDHLDYHGTMEAYFEAKRRLFALLSAAKTAVVNVDDPYGRRLAADLPPGVAVITYSLREPGAEVRGALADPAGLRLAAHTPWGPAEVRLPLAGRHNVYNALAAAAAALCEGIPLEAILAGLATLTPPPAGRLERVDEGQEFVTLVDYSHKPGAVEAVLTALRPVTEGDLTVVIGCGGDRDRGKRPLMGEAAARLADTAIFTDDNPRSEDPLAILAAMVEGGLKVPQPERAHIVVEPDRAAAIALAVARARRGDVLVIAGKGHEDYQILGTQKIHFDDREAARRALVARGAGP",
    "response#2": "MILLKDILYKVTLTAVSGTTSVAEITGVTADSRKAAKGFLFAALPGTKVDGRAFAAKAIEAGAAAVIADGRRAMGPLADIFYGNPSSKLLLIGITGTNGKTTSAFIIKNILQSNGYKVGLIGTIMNKIGDEVIEAEHTTPESYDLQKLFAEMVDKKVDACVMEVSSQALMLDRTAGILFDYGIFTNLEPDHIGPNEHASFEEYLACKGLLFKQCRIGIVNIDDAHTKNVLEGHTCSLETYGFSKGADLRAENIRLMNYKGKSYIHIHYKGELDMEVETSLIGKFNVYNALAAIAVCHMEGIPMEKIKEGLYQVSVKGRMQLVAQRENGATVFVDYAHKPGAVIAALGSLRPHVMGRIVCVIGAGGDRDRGKRSLMGQAARDFADVVIVTDDNPRTEDPAAIRAAVMEGAGPEATEIPDRAEAILRGVDALLPGDALLICGKGHEDYQILKDKTIHMDEREIVAEIIKEKEA",
    "response#3": "MRISGLSSDSRKTKPGDLFAALTGSQSDGRAYVDQAVAAGAVAVLAEPGLPPCGVPVIEVPHARAALSRAASRFYGRPAAALPLIGITGTKGKTTTTYLIRSMLENAGHRTGLIGTIETIIGDRVLPSHNTTPASEVLHQLFREMRDAGCRYAVMEVSSHALALDRVYGLRFDVGVFTNLTQDHLDFHRDMESYFRAKKRLFDSLDPDAAAVINIDDEAGRRIVRDYHRSVRTYGIDGPAEVRALNITSSADGTDFVVGWPGREEPVRLHTRLVGRFNVYNALAAAACALAEGIPMEAVKAALEGIDGVPGRMQLVAQRENGATVFVDYAHKPGAVIAALGSLRPHVMGRIVCVIGAGGDRDRGKRSLMGQAARDFADVVIVTDDNPRTEDPAAIRAAVMEGAGPEATEIPDRAEAILRGVDALLPGDALLICGKGHEDYQIIGERKVHFDDREEALAALRERRERG"
  },
  {
    "reference": "MQTRAIILAAGKGTRMKSRKAKVLHELCGLPMIQHVINNVKEAGFEEVHTIIGPDSQEVGEYLGGSVEKTVQKEQLGTAHAVNQLSGALSGQDGDTLVICGDTPLISPLTIKNLLEFHRERKSLATILSAEAEIPTGYGRVVRNYDGSVKRIVEEKDATSEIRELHEISTGTFIFDNQALFKALEKVTAENAQNEYYLPDVISIMFSEHKLIDAYETAGFNETMGINDRVALARAEELLREQINIRHMQNGVSFMDPKATYVDATVKIGMDTLIYPGAVLSGDTTIGENVTITTASHITDSHIEDDAVIKQSVLSGARVGKGTTVGPYAQLRPGAKLGENVKVGNFVEVKKSELEDESKVSHLSYIGDAYIGERTNIGCGTITVNYDGKNKYRTEIGKDSFIGCNANLVAPVRIGDRSFIAAGSTVTEEVPDDSLAIARNRQTTKEGYYKNSK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Mg(2+), acetyl-CoA binding site should be located in a region of the protein that is accessible to the ligand.2. The ideal protein would be one that plays a critical role in lipid A biosynthetic process, peptidoglycan biosynthetic process through glucosamine-1-phosphate N-acetyltransferase activity within cytoplasm. The designed protein sequence is ",
    "response#1": "DLAEAEAAFQARRRQEMMESGVTLIAPDTVFFSHDTRIAADVVVEPNVVFGPGVNVASNVRIKAFSHLEGASLGEGAEAGPFARLRPGAELGANAKVGNFVEVKKARLGEGAKASHLTYLGDTDVGAHANIGAGTITCNYDGFFKHRTVIGEGAFIGSNTSLVAPVTIGAGAMTGSGSVITEDVPDNALALSRARR",
    "response#2": "LILAAGAGTRMKSDTPKVLHTLGGRSMLAHALHSVAELDPRHVVIVVGKDREKVTPAARQLAEQLGRDIEIAVQEQQLGTGHAAECGLGALPPDFTGTVVVTSGDVPLLDAATLAGLIATHDANGAAVTVLTTTPDDPTGYGRILRTTEGAVTGIVEQADATPEQQAITEVNAGVYAFDAAALRSALSRLQSDNAQHELYLTDALAIARADGRVEAIRQEDERELSRINTKAQMAEARAILRNRLLEKDLAEAEAAFQARRRQEMMESGVTLIAPDTVFFSHDTRIAADVVVEPNVVFGPGVNVASNVRIKAFSHLEGASLGEGAEAGPFARLRPGAELGANAKVGNFVEVKKARLGEGAKASHLTYLGDTDVGAHANIGAGTITCNYDGFFKHRTVIGEGAFIGSNTSLVAPVTIGAGAMTGSGSVITEDVPDNALALSRAREVKRIEGYGVTMDDPATTWIDATVKVAPDVHLHPGVQLQGATAVDSGAEIGPDTTLTDTIVGAGAEVVRSHATQAHIGPGAHVGPFAHLRPASKLAERSKIGAFVETKNLEMGAGAKAAHLAYLGDVTVGADANIGCGVIVANYDGVKKHRTEIGEGAFVGCDSVLVAPVSVGDGAYIGAGSTIVKDVEPGDLAVARARGVPARVIRQR",
    "response#3": "GATLTAPDTVFFSHDTRIGRDVLIEPHVVFGPGVSVADGAVIHAFSHLEGAEVGPGATVGPYGRLRPGAKLAEKAKVGNFVEIKAAEIGAGAKVSHLTYIGDATVGAEANIGAGTITCNYDGFFKYRTTIGAGAFVGSNSSLVAPVTIGAGAFVGSGSVVTEDVAPDALAVARGRR"
  },
  {
    "reference": "MELPNQHKQTAAEGFVSFLNWLCNPWRRQRTVNAAVAFQKDLLAIEDSEHLDDINECFEESAGAQSQRTKVVADGAYAPAKSNRTRRVRKQKKHKFVKYLVNEARAEFGLPKPTEANRLMVQHFLLRVCKDWGVVTAHVHGNVALALPLVFIPTEDDLLSRALMNTHATRAAVRGMDNVQGEGWWNNRLGIGGQVGLAFRSKXGCLERRPGFSTSVSRGEHPDLVVIPSGRPEKQRQLLRYSGIGGHLLIGIHNNSLSNLRRGLMERVFYVEGPNGLQDAPKPVKGAFRTLDKFRDLYTKNSWRHTPVTSEQFLMNYTGRKLTIYREAVDSLSHQPLSSRDAKLKTFVKAEKLNLSKKPDPAPRVIQPRSPRYNVCLGRYLRHYEHHAFKTIAKCFGEITVFKGFTLEQQGEIMRSKWNKYVNPVAVGLDASRFDQHVSVEALEYEHEFYLRDYPNDKQLKWLLKQQLCNVGTAFASDGIIKYKKKGCRMSGDMNTSLGNCILMCAMVYGLKEHLNINLSLANNGDDCVIVCEKADLKKLTSSIEPYFKQFGFKMEVEKPVDIFERIEFCQTQPVFDGSQYIMVRKPSVVTSKDVTSLIPCQTKAQYAEWLQAVGECGMSINGGIPVMQNFYQKLQTGIRRTKFTKTGEFQTNGLGYHSRYMHRVARVPSPETRLSFYLAFGITPDLQEALEIFYDTHKLELDDVIPTDTYQVSGEHLINGLPN",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein with RNA binding, RNA-dependent RNA polymerase activity is needed to enhance viral RNA genome replication in a specific context. The designed protein sequence is ",
    "response#1": "IFSKTQHKVNENSLFTSWKACQTLALMHDYLILVLGPVKKYQRILDSRDRPAHLYIHAGQTPHQLSEWCQNHLTPSVHLANDYTAFDQSQHGEAVVLEAWKMRRASIPEPFITLHVHVKTNIECQFGPLTCMRITGEPGTYDDNTDYNLAILYTQYLLHRTPVLVSGDDSLVDRVPPMNPSWPALAPLFALKPKPETSPFGLFCGYFV",
    "response#2": "IFSKTQHKVNENSLFTSWKACQTLALMHDYLILVLGPVKKYQRILDSRDRPAHLYIHAGQTPHQLSEWCQNHLTPSVHLANDYTAFDQSQHGEAVVLEAWKMRRASIPEPFITLHVHVKTNIECQFGPLTCMRITGEPGTYDDNTDYNLAILYTQYLLHRTPVLVSGDDSLVDRVPPMNPSWPALAPLFALKPKPETSPFGLFCGYFV",
    "response#3": "IFSKTQHKVNENSLFTSWKACQTLALMHDYLILVLGPVKKYQRILDSRDRPAHLYIHAGQTPHQLSEWCQNHLTPSVHLANDYTAFDQSQHGEAVVLEAWKMRRASIPEPFITLHVHVKTNIECQFGPLTCMRITGEPGTYDDNTDYNLAILYTQYLLHRTPVLVSGDDSLVDRVPPMNPSWPALAPLFALKPKPETSPFGLFCGYFF"
  },
  {
    "reference": "MAAKQGSFRHGILEKRERLLSNNGFSDFRFTDIESNDLLENENCGRRTRLCCCCSCGNLSEKISGVYDDAKDVARKAWEMGVSDPRKIVFSAKIGLALTIVALLIFYQEPNPDLSRYSVWAILTVVVVFEFTIGATLSKGFNRALGTLSAGGLALGMAELSTLFGDWEEIFCTLSIFCIGFLATFMKLYPSMKAYEYGFRVFLLTYCYILISGFRTGQFIEVAISRFLLIALGAGVSLGVNMFIYPIWAGEDLHNLVVKNFMNVATSLEGCVNGYLRCLEYERIPSKILTYQASEDPVYKGYRSAVESTSQEESLMSFAIWEPPHGPYKSFNYPWKNYVKLSGALKHCAFTVMALHGCILSEIQAPEERRQVFRQELQRVGVEGAKLLRELGEKVKKMEKLGPVDLLFEVHLAAEELQHKIDKKSYLLVNSECWEIGNRATKESEPQELLSLEDSDPPENHAPPIYAFKSLSEAVLEIPPSWGEKNHREALNHRPTFSKQVSWPARLVLPPHLETTNGASPLVETTKTYESASALSLATFASLLIEFVARLQNVVDAFKELSQKANFKEPEIVTTGTDVEFSGERVGLGQKIRRCFGM",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. The protein design should be able to enhance the efficiency of malate transport.3. The protein must exhibit the following characteristics: Vacuolar malate channel; Exhibits also a weak chloride conductance.; Has a higher selectivity for malate than for fumarate. The designed protein sequence is ",
    "response#1": "MVLSSFFVHILIMGSQMALGVLNVEWLEEFHQSRGLTAWVSSLSMGITLIVGPFIGLFINTCGCRQTAIIGGLVNSLGWVLSAYAANVHYLFITFGVAAGLGSGMAYLPAVVMVGRYFQKRRALAQGLSTTGTGFGTFLMTVLLKYLCAEYGWRNAMLIQGAVSLNLCVCGALMRPLSPGKNPNDPGEKDVRGLPAHSTESVKSTGQQGRTEEKDGGLGNEETLCDLQAQECPDQAGHRKNMCALRILKTVSWLTMRVRKGFEDWYSGYFGTASLFTNRMFVAFIFWALFAYSSFVIPFIHLPEIVNLYNLSEQNDVFPLTSIIAIVHIFGKVILGVIADLPCISVWNVFLLANFTLVLSIFILPLMHTYAGLAVICALIGFSSGYFSLMPVVTEDLVGIEHLANAYGIIICANGISALLGPPFAGWIYDITQKYDFSFYICGLLYMIGILFLLIQPCIRTR",
    "response#2": "VGRFQAGDYVVFACLFVVSSGIGVFFAIKERNKAPSKEFLVGGRQMSCGPVALSLTASFMSAVTVIGAPADVYRFGASYVIFGVAYTFVVFFTAELFLPVFYRSGITSTYEYLELRFCKLVRVAATLIYIIQTILYTGVVVYAPALALNQVTGFDLWGSIFATGIVCTFYCTLGGLKAVVWTDAFQMVVMVVGFLTVLIQGSSRAGGIENVWSTSRTGGRLQVFDFDVSPLRRHTFWTLSVGGTFTWLGIYGVNQSTIQRCISCKTEGHARWALYLNLLGLWIILFCAVVSGLIMYSYYSHCDPWSSGLISAPDQLMPYFVMEILGAFPGLPGLFVACAFSGTLSTVAASINALATVMYEDFVSQCFPDLSNRAASWISKALCVAFGVACTTMAVAASYMGGIVQAALSIHGMCGGPVLGLFSLGILFPFTNLKGAVGGLIVGISLSFWVGVGAFIYPAPSNNTHALELNTAGCNITAAAFEPTSATVTQLTSDRNWLADSWYSMSYLYYSAVGFIGTVAAGLLITLLTVGRFQAGDYVVFACLFVVSSGIGVFFAIKERNKAPSKEFLVGGRQMSCGPVALSLTASFMSAVTVIGAPADVYRFGASYVIFGVAYTFVVFFTAELFLPVFYRSGITSTYEYLELRFCKLVRVAATLIYIIQTILYTGVVVYAPALALNQVTGFDLWGSIFATGIVCTFYCTLGGLKAVVWTDAFQMVVMVVGFLTVLIQGSSRAGGIENVWSTSRTGGRLQVFDFDVSPLRRHTFWTLSVGGTFTWLGIYGVNQSTIQRCISCKTEGHARWALYLNLLGLWIILFCAVVSGLIMYSYYSHCDPWSSGLISAPDQLMPYFVMEILGAFPGLPGLFVACAFSGTLSTVAASINALATVMYEDFVSQCFPDLSNRAASWISKALCVAFGVACTTMAVAASYMGGIVQAALSIHGMCGGPVLGLFSLGILFPFTNLKGAVGGLIVGISLSFWVGVGAFIYPAPSNNTHALELNTAGCNITAAAFEPTSATVTQLTSDRNWLADSWYSMSYLYYSAVGFIGTVAAGLLITLLT",
    "response#3": "MVLSSFFVHILIMGSQMALGVLNVEWLEEFHQSRGLTAWVSSLSMGITLIVGPFIGLFINTCGCRQTAIIGGLVNSLGWVLSAYAANVHYLFITFGVAAGLGSGMAYLPAVVMVGRYFQKRRALAQGLSTTGTGFGTFLMTVLLKYLCAEYGWRNAMLIQGAVSLNLCVCGALMRPLSPGKNPNDPGEKDVRGLPAHSTESVKSTGQQGRTEEKDGGLGNEETLCDLQAQECPDQAGHRKNMCALRILKTVSWLTMRVRKGFEDWYSGYFGTASLFTNRMFVAFIFWALFAYSSFVIPFIHLPEIVNLYNLSEQNDVFPLTSIIAIVHIFGKVILGVIADLPCISVWNVFLLANFTLVLSIFILPLMHTYAGLAVICALIGFSSGYFSLMPVVTEDLVGIEHLANAYGIIICANGISALLGPPFAGWIYDITQKYDFSFYICGLLYMIGILFLLIQPCIRH"
  },
  {
    "reference": "MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYATADWLNRKFSIIDTGGIDDVDAPFMEQIKHQAEIAMDEADVIVFVVSGKEGITDADEYVARMLYKTHKPIILAVNKVDNPEMRNEIFDFYALGLGDPFPVSSVHGIGTGDVLDAIVENLPNEEVAENPDMIKFSLIGRPNVGKSSLINAILGEERVIASPVAGTTRDAIDTVFTDSEGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVLNAEEGIREYDKRIAGFAHEAGKGIVIVVNKWDTLEKDNHTMKDWEADIRDQFQYLSYAPIIFVSALTKQRLHKLPDMIKQISQSQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKPPTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The GTP binding site should be stable and able to withstand changes in environmental conditions.2. The protein must be able to GTP binding. The designed protein sequence is ",
    "response#1": "SNELVRPPIANIDQAIIVSSAVDPDFTTLLLDRFLVLIESKHIQPIILITKVDLLNEDQLENIKLYKQHYEKIGYRVELVSSKNQDLLPELEPYFDEKVSVFAGQSGVGKSSLINVLDPSLLLETAEISKSLGRGKHTTRHVELMKIGNGLVADTPGFSVG",
    "response#2": "VGIGGPVGAGKTTLTAALSEALRDSHSIGVITNDIYTREDAEALMRLQILPQDRIIGVETGGCPHTAIREDASINLAAVAEMERRHPGIEIILIESGGDNLSATFSPELADVTLYVIDVAAGEEIPRKGGPAITKSDILIINKTDLAPHVGASLEVMERDAGRMRCGRPFL",
    "response#3": "KDLLRFLTCGSVDDGKSTLIGRLLYDTKLLFDDQVATLERESKSRGGAGQEIDFSLLVDGLEAEREQGITIDVAYRFFSTAKRKFIVADTPGHEQYTRNMATGASNADLAIILVDARKGVLTQTRRHSFIASLLGIRHVVLAVNKIDLVGYSQSRFEETVADYERFARDLSFETVKAIPVSAKLGDNVISRGANLDWYAGPHLLGYLEAVEVARDAFE"
  },
  {
    "reference": "MTTIRRFVCDDLFKFNNINLDYLTETYYLPFYLQYLSKWPSLLSMAEDVNGKPMGYMIGKAEGEGINWHGHVTAVSVAPEFRRIGLADRLMHILEEGSEKIYDGYFVDLFVRKSNTLAINMYTKFGYSVYRTVIGYYSGDEDALDMRKALPRDVEKKSIIPLKHPVYPTDADL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have peptide alpha-N-acetyltransferase activity within NatB complex, cytoplasm to regulate N-terminal peptidyl-methionine acetylation. The designed protein sequence is ",
    "response#1": "NNLGMLTKLAHVTVPNLYQDAFFSALFAEDSLVAKNKKPSSKKDVHFTQMAYYSEIPVGGLVAKLVPKKQNELSLKGIQIEFLGVLPNYRHKSIGSKLLKFAEDKCSECHQHNVFVYLPAVDDLTKQWFIAHGFEQVGETVNNFIKGVNGDEQDAILLKKHIL",
    "response#2": "TTLRAFTCDDLFRFNNINLDPLTETYGIPFYLQYLAHWPEYFIVAEAPGGELMGYIMGKAEGSVAREEWHGHVTALSVAPEFRRLGLAAKLMELLEEISERKGGFFVDLFVRVSNQVAVNMYKQLGYSVYRTVIEYYSASNGEPDEDAYDMRKALL",
    "response#3": "NNLGMLTKLAHVTVPNLYQDAFFSALFAEDSLVAKNKKPSSKKDVHFTQMAYYSEIPVGGLVAKLVPKKQNELSLKGIQIEFLGVLPNYRHKSIGSKLLKFAEDKCSECHQHNVFVYLPAVDDLTKQWFIAHGFEQVGETVNNFIKGVNGDEQDAILLKKHIG"
  },
  {
    "reference": "MDFLIASHNKKKRTELERILKPLGINVLIAEQAGIILTDVQETGETFEENALLKAKSGARESNMPCVADDSGLCVDYLKGRPGVYSARYAGEHGNDAKNIEKLLKELQGVPHDKRTARFVSVVACVFPDGREIVCRGECEGKIGLSPAGNGGFGYDPVFYIGNVSFAQLSNEEKDKLSHRGKSLRKLREALL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "SEWFFLDFINPSSANLIKARRLIFSARSPYQEIEILDTQAFGKCLMLDGRMQSAQADEFIYHEALVHPAMVTHPNPESVLIVGGGEGATLREVLRHRTVRRAVMVDIDRQVVEACRLFLPEWSDGAFEDPRTELVIDDARHYLTSTDEQFDVIIMDITEPLENGPSAPLVTYEMFQIVRSHLKPQGIVAHQSGSTGTSELHFFASMYKTLRAVFRYVAPYSIQITSFAMPWGIVIASR",
    "response#2": "SEWFFLDFINPSSANLIKARRLIFSARSPYQEIEILDTQAFGKCLMLDGRMQSAQADEFIYHEALVHPAMVTHPNPESVLIVGGGEGATLREVLRHRTVRRAVMVDIDRQVVEACRLFLPEWSDGAFEDPRTELVIDDARHYLTSTDEQFDVIIMDITEPLENGPSAPLVTYEMFQIVRSHLKPQGIVAHQSGSTGTSELHFFASMYKTLRAVFRYVAPYSIQITSFAMPWGIVIASR",
    "response#3": "SEWFFLDFINPSSANLIKARRLIFSARSPYQEIEILDTQAFGKCLMLDGRMQSAQADEFIYHEALVHPAMVTHPNPESVLIVGGGEGATLREVLRHRTVRRAVMVDIDRQVVEACRLFLPEWSDGAFEDPRTELVIDDARHYLTSTDEQFDVIIMDITEPLENGPSAPLVTYEMFQIVRSHLKPQGIVAHQSGSTGTSELHFFASMYKTLRAVFRYVAPYSIQITSFAMPWGIVIASQ"
  },
  {
    "reference": "MASEDKSKALAAALAQIEKQFGKGSIMRMSDNQITENLQVISTGSLTLDLALGVGGLPRGRVVEIYGPESSGKTTLCLQAVAEAQKLGGTCAYIDAENALDPVYAQKLGVNVEDLLISQPDTGEQALEICDMLVRSSGVDVIVVDSVAALVPKAEIEGEMGDSHVGLQARLMSQALRKLTGNIKRTNTLVIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGGIKKGDEVIGNDTRVKVVKNKVSPPFRQADFEILYGEGISRQGEIIELGVKHGFIDKSGAWYAYNGQKIGQGKDNTREWLKANPAIADEIERKIREAVGVKIEINENQGDEEFADDAFDA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The ATP binding site should be stable and able to withstand changes in environmental conditions.2. The designed protein must be able to regulate SOS response.3. The protein must exhibit the following characteristics: Can catalyze the hydrolysis of ATP in the presence of single- stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. The designed protein sequence is ",
    "response#1": "RGRIVEIYGPESSGKTTLTLHAIANAQRAGGIAAFIDAEHALDPDYARKLGVDIDALLVSQPDTGEQALEIADMLVRSGSIDLIVIDSVAALVPRAEIEGEMGDAHVGLQARLMSQALRKLTGALSQTNTTMIFINQLREKVGVFFGSPETTAGGKALKFYASVRLDIRRIP",
    "response#2": "KGRIVEIYGPESSGKTTLTLQVIAEAQRQGGTCAFVDAEHALDPVYAEKLGVNVDDLIISQPDNGEQALEVADMLVRSGAVDVLVVDSVAALTPKAEIEGDMGDHHVGLQARLMSQALRKITGNIKNANCLAIFINQIRMKIGVMFGNPETTTGGNALKFYSSVRLDIRRIF",
    "response#3": "PVEVVPTGSIALDVALGIGGLPRGRIVEIYGPESSGKTTLTLHAIANAQRAGGIAAFIDAEHALDPDYARKLGVDIDALLVSQPDTGEQALEIADMLVRSGSIDLIVIDSVAALVPRAEIEGEMGDAHVGLQARLMSQALRKLTGALSQTNTTMIFINQL"
  },
  {
    "reference": "MGKENDISKCPFHNGSMKSNVAGGGTQNNDWWPKRLKVSVLRQNSSLSDPMDKDFNYAEAFNSLDLEEVKRDLHALMTDSQDWWPADFGHYGPLFIRMAWHSAGTYRVGDGRGGAGAGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGKKISWADLMILTGNVALESMGFKTFGFAGGREDVWEPEEDVYWGSETTWLGGDIRYGHGSEGVVEGHGVTVSDDDADGDKHTRDLENPLAAVQMGLIYVNPEGPDGNPDPVAAIQDIRDTFGRMAMNDEETVALIAGGHTFGKTHGAASADNVGAEPEGADLELQGLGWVNSYGTGKGGDAITSGLEVTWTKTPTQWSNNFFENLFGYEWELTKSPAGAHQWVAKNADNIIPDAFDGSKKHRPTMLTTDLSLKFDPEYERISRRFYENPDEFADAFARAWYKLTHRDMGPKTRYLGPEVPQEELLWQDPIPEVNHELVNDSDVAALKAKVLESGLSVSELVSTAWASASTFRGSDKRGGANGARIRLAPQKDWLVNNPVQLQKVLGKLEDIQNSFNAAQGGGKKVSLADLIVIAGAAGVEKAARDAGHNITVPVAAGRMDASQQQTDVEQFGYLEPFADGFRNYRKGKSQVSTEELLIDRAQLLTLTAPELTVLLGGMRVLNTNFDGSNYGVFTDRPGQLTNDFFVNLLDMNTEWKATSDERETYVGSDRNTGKAKWVGTRADLVFGSNSELRAVAEVYASADAKDKFVNDFVAAWNKIMNADRFDLA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be able to modulate response to oxidative stress in a way that leads to a desirable outcome.2. For general function, the protein need meet that Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.3. The presence of heme b is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "TTNAGAPVWNDNEALTVGPRGPILLEDYHLIEKVAHFARERIPERVVHARGASAKGFFECTHDVTSLTCADFLRAPGVRTPVIVRFSTVIHERGSPETIRDPRGFAVKFYTREGNWDLLGNNFPVFFIRDGIKFPDVIHAFKPNPRSHVQEYWRVFDFLSHLPESLHTFFFLFDDVGVPSDYRHMEGFGVNTYTFVSAAGKAQYVKFHWKPTCGVRCILTDEEAALVGGRNHSHATQDLYDSIAAGSFPEWTLYVQVMDPDTEEQYDFDPLDDTKTWPEDLLPLRPVGRLVLDRNVDNFFNENEQLAFGPGLVVPGIYYSDDKMLQCRVFAYADTQRYRLGPNYLMLPVNAPRCAHHNNHYDGAMNFMHRDEEVDYYPSRHAPLXD",
    "response#2": "TTNAGAPVWNDNEALTVGPRGPILLEDYHLIEKVAHFARERIPERVVHARGASAKGFFECTHDVTSLTCADFLRAPGVRTPVIVRFSTVIHERGSPETIRDPRGFAVKFYTREGNWDLLGNNFPVFFIRDGIKFPDVIHAFKPNPRSHVQEYWRVFDFLSHLPESLHTFFFLFDDVGVPSDYRHMEGFGVNTYTFVSAAGKAQYVKFHWKPTCGVRCILTDEEAALVGGRNHSHATQDLYDSIAAGSFPEWTLYVQVMDPDTEEQYDFDPLDDTKTWPEDLLPLRPVGRLVLDRNVDNFFNENEQLAFGPGLVVPGIYYSDDKMLQCRVFAYADTQRYRLGPNYLMLPVNAPRCAHHNNHYDGAMNFMHRDEEVDYYPSRHAPLY",
    "response#3": "TTNAGAPVWNDNEALTVGPRGPILLEDYHLIEKVAHFARERIPERVVHARGASAKGFFECTHDVTSLTCADFLRAPGVRTPVIVRFSTVIHERGSPETIRDPRGFAVKFYTREGNWDLLGNNFPVFFIRDGIKFPDVIHAFKPNPRSHVQEYWRVFDFLSHLPESLHTFFFLFDDVGVPSDYRHMEGFGVNTYTFVSAAGKAQYVKFHWKPTCGVRCILTDEEAALVGGRNHSHATQDLYDSIAAGSFPEWTLYVQVMDPDTEEQYDFDPLDDTKTWPEDLLPLRPVGRLVLDRNVDNFFNENEQLAFGPGLVVPGIYYSDDKMLQCRVFAYADTQRYRLGPNYLMLPVNAPRCAHHNNHYDGAMNFMHRDEEVDYYPSRL"
  },
  {
    "reference": "MDLLSIQDPSFLKNMSIDELEKLSDEIRQFLITSLSASGGHIGPNLGVVELTVALHKEFNSPKDKFLWDVGHQSYVHKLLTGRGKEFATLRQYKGLCGFPKRSESEHDVWETGHSSTSLSGAMGMAAARDIKGTDEYIIPIIGDGALTGGMALEALNHIGDEKKDMIVILNDNEMSIAPNVGAIHSMLGRLRTAGKYQWVKDELEYLFKKIPAVGGKLAATAERVKDSLKYMLVSGMFFEELGFTYLGPVDGHSYHELIENLQYAKKTKGPVLLHVITKKGKGYKPAETDTIGTWHGTGPYKINTGDFVKPKAAAPSWSGLVSGTVQRMAREDGRIVAITPAMPVGSKLEGFAKEFPDRMFDVGIAEQHAATMAAAMAMQGMKPFLAIYSTFLQRAYDQVVHDICRQNANVFIGIDRAGLVGADGETHQGVFDIAFMRHIPNMVLMMPKDENEGQHMVHTALSYDEGPIAMRFPRGNGLGVKMDEQLKTIPIGTWEVLRPGNDAVILTFGTTIEMAIEAAEELQKEGLSVRVVNARFIKPIDEKMMKSILKEGLPILTIEEAVLEGGFGSSILEFAHDQGEYHTPIDRMGIPDRFIEHGSVTALLEEIGLTKQQVANRIRLLMPPKTHKGIGS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a binding site that is specific to the target Mg(2+) ligand.2. The protein should be optimized for activity in Metabolic intermediate biosynthesis, 1-deoxy-D-xylulose 5-phosphate from D- glyceraldehyde 3-phosphate and pyruvate: step 1/1 pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The protein design should be able to enhance the efficiency of thiamine biosynthetic process, isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway.4. The protein should be designed to catalyze the following reaction D-glyceraldehyde 3-phosphate + H(+) + pyruvate = 1-deoxy-D-   xylulose 5-phosphate + CO2. The designed protein sequence is ",
    "response#1": "MSYTQVFSQWLCDMAAQDGLLVGITPAMREGSGLVKFSECFPERYFDVAIAEQHSVTLAAGMACDGLKPVVAIYSTFLQRAYDQLIHDVALQNLPVLFAIDRAGVVGPDGPTHAGSFDLTYLRCIPNLVVMAPADENECRQMLYTGFLLNQPAAVRYPRGKGPGEFGRLMCNPGEDGPTHAGAFDISFLGKGRELRQGKTVCFINFGVLLPDAIAVAEANNYGVCDMRWAKPLDKDLLLNMAEQYDYLVTLEENAVAGGAGAGVMELLAAEGISTPVLPLGLPDEYLDHGKRSQLLQAAGLDRASDAILECIRG",
    "response#2": "MSYTQVFSQWLCDMAAQDGLLVGITPAMREGSGLVKFSECFPERYFDVAIAEQHSVTLAAGMACDGLKPVVAIYSTFLQRAYDQLIHDVALQNLPVLFAIDRAGVVGPDGPTHAGSFDLTYLRCIPNLVVMAPADENECRQMLYTGFLLNQPAAVRYPRGKGPGLGRLAAPQEVQPAPDVLLVAVGPMAAACLDAAALLAADGITATVVDPCWVKPVDPALVELAGAHRMVVTVEDNGRTGGVGAALAQAMRDADVDTPLRDLGIPQEFLAHASRGEILEEIGLTGSSPMAALTAVLGLPAWSKVFSDTVLSFAKEDKEVVAITPAMPVGSKLTRFQSELPKQFFDVGIAEQHAVTMAAGLAMNGMKPYVAIYSTFLQRAYDQVLHDVDRQNLHVVFGVDRSGLVGADGETHQGVFDIPFLSHLPNMTIMMPKDEQEAYQMLYNAFYQYDGPIAIRYPRGGKAEILKTGISNKLFIGYGAGVSRAIDTEALHNEDITVVDLRFVKPLDKNLLLELSKKYNDWYIFSDSQKQSGVGSAILEFLNDEKIHNVQLTSFEYNDKFIEHGDTKLVEQSLGLLPELASPRRGKGVCRRRGRDVALLAFGSLVAPALTAAERLDATVADMRFVKPLDVDLVGELAAGHRLLVTLEENAVAGGAGAAVAELLAQLGIVVPVLQLGLSDVFIEHGDAAAMLAACGLDAPGSVEAAAALVLQVLRD",
    "response#3": "MSYTQVFSQWLCDMAAQDGLLVGITPAMREGSGLVKFSECFPERYFDVAIAEQHSVTLAAGMACDGLKPVVAIYSTFLQRAYDQLIHDVALQNLPVLFAIDRAGVVGPDGPTHAGSFDLTYLRCIPNLVVMAPADENECRQMLYTGFLLNQPAAVRYPRGKGPGLGRLAVPTPKLTYTQVFSDWLCDIAAADPRIVGITPAMREGSGLVEFAQRFPDRYYDVGIAEQHALTFAAGLACEGLKPVVAIYSTFLQRAYDQLIHDIALQNLPVVLAIDRGGLVGADGATHHGAFDLSFLACVPNLVVMAPADENECRQMLYTAVCHDGPTAVRYPRTGPDGPTHAGSFDLTYLGKWEVLRKGTDAVILSFGPTLQMLLEAQETLLKEGINIEIVNARFIKPLDEDYLDKLAERNVPVLTVEESMLTGGFGSLVVNYFNDHHQCVTVRRLGIDNEYIEQGDVDMLLQEIGLTTAHLQADILQAAAKLTYTQVFSDWLCDIAAADPRIVGITPAMREGSGLVEFAQRFPDRYYDVGIAEQHALTFAAGLACEGLKPVVAIYSTFLQRAYDQLIHDIALQNLPVVLAIDRGGLVGADGATHHGAFDLSFLACVPNLVVMAPADENECRQMLYTAVCHDGPTAVRYPRT"
  },
  {
    "reference": "MKIGIINTKIRTVFSAFACMIAASLVCTMPARAVVEININKGVIEPLPIAITDFLSADQLGSNITSVIAADLERSGLFAPIDKGAFIEKISNPDAAPRFEDWKVINAQALVTGRITKQPDGRLKAEFRLWDTFGGQQMIGQQFFTTPDNWRRVAHIIADAIYERLTGDKGYFDTRVVFVDESGPAQKRVKRLAIMDQDGANVRFISDGRALSLTPRFSPNRQEVTYMSFEGGSPKVYLLQLETGQRELVGNFPGMTIAPRFSPDGQKVVMSLLQDDGSANIYTMDLRNRTTTRLTSSQAIDTGASYSPDGSQIVFTSDRGGRPQLYVMGADGSNPRRISMGDGSYSTPVWSPRGDLIAFTKQSQGQFSIGVMKTDGSGERLLTSGFHNEGPTWAPNGRVLMFFRKAAGAGGPKLFTIDLTGRNERQIQTPNFASDPAWSPLLE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be optimized for stability and expression in periplasmic space where it will participate in cell cycle, cell division, protein import.2. The protein must exhibit the following characteristics: Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity.. The designed protein sequence is ",
    "response#1": "MLNRRNFIRTTSALAASTALPGYAFM",
    "response#2": "MLNRRNFIRTTSALAASTALPGYAFM",
    "response#3": "VPTPTSAGSTSALGQALAQVISANLRNNGLFKPVGPESLPRPGFEQVQAPDFAVWSARSAEMLVQGYVRANGDGQLTVGCYLYDVALKQQR"
  },
  {
    "reference": "MSGHDSKYFSTTKKGEIPELKEELNSQYKDKRKDAVKKVIAAMTVGKDVSSLFTDVVNCMQTENLELKKLVYLYLINYAKSQPDLAILAVNTFVKDSQDPNPLIRALAVRTMGCIRVDKITEYLCDPLQKCLKDDDPYVRKTAAICVAKLFDINAELVEDRGFLEALKDLISDNNPMVVANAVAALAEIQENSTSPIFEINSTILTKLLTALNECTEWGQVFILDALSRYKASDPREAENIVERVTPRLQHANCAVVLSAVKMILQQMELITSTDVIRNLCKKMAPPLVTLLSAEPEIQYVALRNINLIVQKRPTILAHEIKVFFCKYNDPIYVKMEKLEIMIKLASDRNIDQVLLEFKEYATEVDVDFVRKAVRAIGRCAIKLERAAERCISVLLELIKIKVNYVVQEAIIVIKDIFRRYPNTYESIIATLCESLDTLDEPEAKASMIWIIGEYAERIDNADELLESFLENFPEEPAQVQLQLLTATVKLFLKKPTEGPQQMIQVVLNNATVETDNPDLRDRAYIYWRLLSTDPEAAKDVVLAEKPVITDDSNQLDPSLLDELLANISTLSSVYHKPPEAFVTRLKTTVQKTEDEDYVEGSETGYPEASGNPVDGAASPSATTGYVTKLAAAPAPVPDLLGDLMGSDNAAIVPVDEPTTPSGRPLPVVLPASKGQGLQISAQLTRQDGQVFYSMLLENNSQSLLDGFMIQFNKNSFGLAAVGSLQVPPLQPGASARTMMPMVLSQNMSTGSTSSVLQVAVKNNQQPV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules .; Subunit of clathrin-associated adaptor protein complex that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes.2. The protein should be designed to localize specifically to plasmodesma, Golgi apparatus, clathrin-coated vesicle membrane in order to perform its role in intracellular protein transport, vesicle-mediated transport more effectively. The designed protein sequence is ",
    "response#1": "ISWRTKGIHYAKNEFFLDVIERVQYLMDFEKGVIRKNLIHGEIVCRCYLSGMPKLKISINKILNRDPQFMSNSSFHQCVSLDSINTIEKDEEKNSDDDAGLQAATDAREIEFIPPDGEFVLCQYELKRHVKDAPMVRLKDFEIKPKLKKFKIQIVTKIQTNFKPTNSTSKLNVRIPLTKVFQEYKIDLSKQIRFKANIGKVVFNLSDDFLLWEIQTMKGHREHSTNKSSQYNSDEDDPNTCASMVAEFPLFNQEEYDRLQEEMKTSMNPPPLRTGPRLEELYRQVHDQQTSHVTPRDKLVNIDFEIPYCTCSGLKVEYLKVEEPQLQYQSFPWVRYKTVSDEEYAS",
    "response#2": "ISWRTKGIHYAKNEFFLDVIERVQYLMDFEKGVIRKNLIHGEIVCRCYLSGMPKLKISINKILNRDPQFMSNSSFHQCVSLDSINTIEKDEEKNSDDDAGLQAATDAREIEFIPPDGEFVLCQYELKRHVKDAPMVRLKDFEIKPKLKKFKIQIVTKIQTNFKPTNSTSKLNVRIPLTKVFQEYKIDLSKQIRFKANIGKVVFNLSDDFLLWEIQTMKGHREHSTNKSSQYNSDEDDPNTCASMVAEFPLFNQEEYDRLQEEMKTSMNPPPLRTGPRLEELYRQVHDQQTSHVTPRDKLVNIDFEIPYCTCSGLKVEYLKVEEPQLQYQSFPWVRYKTVSDEEYAG",
    "response#3": "ISWRTKGIHYAKNEFFLDVIERVQYLMDFEKGVIRKNLIHGEIVCRCYLSGMPKLKISINKILNRDPQFMSNSSFHQCVSLDSINTIEKDEEKNSDDDAGLQAATDAREIEFIPPDGEFVLCQYELKRHVKDAPMVRLKDFEIKPKLKKFKIQIVTKIQTNFKPTNSTSKLNVRIPLTKVFQEYKIDLSKQIRFKANIGKVVFNLSDDFLLWEIQTMKGHREHSTNKSSQYNSDEDDPNTCASMVAEFPLFNQEEYDRLQEEMKTSMNPPPLRTGPRLEELYRQVHDQQTSHVTPRDKLVNIDFEIPYCTCSGLKVEYLKVEEPQLQYQSFPWVRYKTVSDEEYAS"
  },
  {
    "reference": "MRPALRTCHAATRNRLFRSGAALDHHREFFGRTRVAVFLGSRDRSTWRRDRVPRDFDRSRRAHTFAPGAIHERRERIRRRAALLLSLRNVVCGFRNGRYPRALSIDAGRIPGTNTGRRRCQRRHGRGHRTAHRWHRPRARTPVRRGSRHRHSFDPFGSLTGLSCVLRERGDHPGHQLVADTRLWAGSQLDHRFDQENPFARSARFPTENALELPLNTQDKSPTGGAASRLLLLRFSGDLTTKADATRRRMTSRLIENLRASIKASGRKAKVTRDRNRIFVELDESPPDGAGPSDEEIVTRMARIFGVESVSLTHVEEWSTLEDVVRAGKIFGEDFVRDKRFAIRARRVGDRNEVPLRSPDIERELGEALRPFASRVDLSNPEATVFVEVDAHHAYFFSASKRAAGGLPLGTEGRAVSLLSGGFDSPVASWRMLRRGVALDYVFCNLGGRTHQLETLTIAKKLADDWQSGTRPRFHAIEFDPVSREIQAKVTMRYWQIILKRMMLRAAEAVAGRYDAAAIITGEAIGQVSSQTLQNLTVIEYGARLPILRPLVGNNKNEIIAESKVVGTHDLSAKVGEYCAIVPSKPATNARLADILEEEAKLDPRVLEEALESRSEFMLADLDLSLWDDEDLRTREIQSTDTVIDLRTKAAFDTWHYPDSLVLDFSNALRAYGSFDPSQNYVLYCEFGLKSAHLADLMRRIGLNARHVSGGLREARRIAEDGNQN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must be able to perform tRNA U4 sulfurtransferase under specific conditions. The designed protein sequence is ",
    "response#1": "MGTQEDVLSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQVSHFLWKKYGSSAKVKFISVDFEPVVAEILEKVENGQMGVVLKRMFMRAAGQVAERFGIQALVTGEALGQVSSQTLTNLRMIDNVTDTLILRPLINWDKEDIINVAREIGTEEFAKTMPEFCGVISKNPTVKH",
    "response#2": "MGTQEDVLSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQVSHFLWKKYGSSAKVKFISVDFEPVVAEILEKVENGQMGVVLKRMFMRAAGQVAERFGIQALVTGEALGQVSSQTLTNLRMIDNVTDTLILRPLINWDKEDIINVAREIGTEEFAKTMPEFCGVISKNPTVKS",
    "response#3": "MGTQEDVLSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQVSHFLWKKYGSSAKVKFISVDFEPVVAEILEKVENGQMGVVLKRMFMRAAGQVAERFGIQALVTGEALGQVSSQTLTNLRMIDNVTDTLILRPLINWDKEDIINVAREIGTEEFAKTMPEFCGVISKNPTVKH"
  },
  {
    "reference": "MSMKHAFLVSALAAVVAAALPVSPVLAETQATTNSFWWPDQLNLKPLRQNAAESNPLGQAFNYAEQFKTLDLKAVKKDIARVLHTSQPWWPADYGNYGPFFIRMAWHSAGVYRIFDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKQQYGSKLSWADLMVLAGNVALEDMGFKTLGFAGGRADDWEAEIVDWGAEKTFLADERHDAKGALAKPLAAVQMGLIYVNPEGPGGKPDPLLAARDIRETFGRMAMNDEETVALIAGGHTFGKAHGAHDPAKCLGKEPAAAGLEEQGLGWANRCGTGHGADTVTSGLEGAWSTNPVRWTHEYLTWLYSFEWETTKSPAGATQWIPKDGQGANFVPDAHIPGKRHTPIMFTTDLALKLDPEYQKISKRFLDHPKEFERAFAQAWFKLTHRDMGPKARYVGAEVPAEEFVWQDPIPKVDHKLLDAKDVARLKAAIVASDLTIPERVRTAWASAATFRGTDMRGGANGARIRLAPQKDWPVNNPAELANVLAKLEILQSDFNRTLKGGKKVSLADVIVLAGCVAVEQAAEKAGVTVQVAFKPGRMDAAQEQTDAQSFEVLHPKADGFRNHFASDNAHSPTTLLVERANLLTLTVPEMTVLLGGMRALDANSGHSRHGVFTERPGTLTNDFFVNLLDMTTQWSKSSTEGIYEGRDRASGQLKWTATPVDLIFGSHSELRAVAEVYASDDAKQKFVQDFAHAWVKVMNLDRFDAR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a high specificity for heme b binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "VSMADLIVLAGCAGVEQAAKAGGHQIEVPFTSGRGDASAEQTDAESFAVMEPVMDGFRNYQARELSTSPEEMLVDRAQLLGLSAPEMTVLVAGLRVLGANHGGSAHGVLTNRPGVLSSDFLTNLLDMGTEWKPSGKGVYEGRDRASGAARWTATRVDLVFGSNSQLRALAERYAQDDAEASFVADFVAAWVKR",
    "response#2": "PADPMKTPMSIVPEWYLLPFYAILRSIPNKLFGVIAMFFAIFILFFLPLLDFNYIRGNKFQPIGKLLFWCFISNFILLMFIGAKHVEIPYVAIGTYATLFYN",
    "response#3": "VSMADLIVLAGCAGVEQAAKAGGHQIEVPFTSGRGDASAEQTDAESFAVMEPVMDGFRNYQARELSTSPEEMLVDRAQLLGLSAPEMTVLVAGLRVLGANHGGSAHGVLTNRPGVLSSDFLTNLLDMGTEWKPSGKGVYEGRDRASGAARWTATRVDLVFGSNSQLRALAERYAQDDAEASFVADFVAAWVKR"
  },
  {
    "reference": "MLHITPLAAFNDNYIWVFQSQQNTGAESSGIYVVDPGDGQVVIDYLIQTGQALLGILITHHHHDHTGGIEQLIRRFGAQIAVYGPQSENIIGVNHPIVANGDISLTNAGLNAKVIQLPGHTLGHIAYLIEDVLFCGDTLFSAGCGRIFEGSAEQMYQSLSELGQLPDNTKVCCAHEYTIANLAFANRVEPNNAALIDYTKKAQALRAQNLPTLPSSIGQEKAINPFLRSDSEEICQSLSIQFQQPINNPLQSFSLLRQWKDNF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be designed to catalyze the following reaction an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy   carboxylate + glutathione + H(+).2. The designed protein must possess hydroxyacylglutathione hydrolase activity to support methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione under certain conditions.3. A protein that is capable of catalyzing reactions within the (R)-lactate from methylglyoxal: step 2/2 pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "EYTKSNALFALTLDPYNQKLQQRAEDVFLLRAKNAMTLPVTLGQEKKNNPFLRWDNRTLRKTLRMEKETDEEVFAEIRKRKDNP",
    "response#2": "DNYIWLIRHENHCLVVDPGDAGPVLDRLAALDLQLDAILLTHHHQDHVGGVTALLKHFPHARLYGPKHDPMPDHHGQWLDDGDQINWHGLSLDVIHVPGHTHGHIAYHGHGMLFCGDTLFSGGCGRLFEGTPAQMHDSLQRLAALPDDTLIYCAHEYTLANLHFAKAVEANNPALIEYTVKAHALRAENKPTLPSSIALEKAINPFLRLDSLEIQKSLAVQFQQPIADPVQSFALLRQWKDNFN",
    "response#3": "IPAFQDNYIWVLTNDEGRCVIVDPGEAAPVLKAIAEHKWMPEAIFLTHHHHDHVGGVKELLQHFPQMTVYGPAETQDKGATHLVGDGDTIRVLGEKFTLFATPGHTLGHVCYFSHPYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTLICCASEYTLANLHFAKAVEANNPALIEYTVKAHALRAENKPTLPSSIALEKAINPFLRLDSLEIQKSLAVQFQQPIADPVQSFALLRQWKDNF"
  },
  {
    "reference": "MKIAVFASGEGTNLQALLDACADGRVRGELALVISNKEDAGALRRARRAGIEALMTPTASYPTPDEYSAYLAMECKNRSIGLICLAGFLMKIKPPLLKAFPGRILNIHPSLLPAFGGQGMYGRKVHEGALAAGVQVSGATVHVVDEEYDRGPIILQASVPVLPNDTPETLAARVRYQEHYLYPKAVALFCDGRVKIADKKVQLLPSPLEASPRVKRALISVSDKTGVVELAKGLHELGVEIVSTSGTAKTLSEAGIPIRPLDSMTGFPEILDGRVKTLHPHVHGAILLRRSDPKQAREAELFGLEPIDLVAVNLYPFAKTAAAASSAYDPAVIEKIDIGGVALIRAAAKNFEDVAVLTSPADYASALAELTASQARLCDSTRRKLALAAFRHTADYDGMIARAWCGEMRCDALRETFSPLLTTRLTKVQDLRYGENPHQKAALYANENGMSFTQLHGKELSYNNLLDASGTWEAVSDFEIPTAVVFKHVTPAGIGSGETIELAFERSWACDPLSAFGGALAFNRPVSRVLAELLFKRFVEVLVAPGYEPEALEIFKKKPNLRLLVRTKAPTHSLQLRSIGDEVLVTEPDRAVAGPDWKVVTKRAPTPVEEKALRFAWTAGKHVKSNAIVLAGPEQTVGIGAGQMSRVDSVHMSGVKYKLWRRDNPAPKALVLASDAFFPFRDGIDAAATLGISAVAQPGGSVKDAEVIAAADEHGLAMVFTGIRHFRH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Catalyzes the transfer of a formyl group from 10- formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate.2. The protein with the reaction H2O + IMP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-   carboxamide, the Proton donor must be optimized to ensure efficient catalysis.3. The protein should be optimized for activity in IMP biosynthesis via de novo pathway, N(2)- formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D- ribosyl)glycinamide (10-formyl THF route): step 1/1 pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The protein should have a high affinity for N(1)-(5-phospho-beta-D-ribosyl)glycinamide, (6S)-10-formyltetrahydrofolate.5. The presence of MGS-like domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "IMLLGSGELGKEVAIEAQRLGIEVIAVDKYENAPAHLVANRSYAIDMQDKSAVLELIEKEQPSYILPEVEAISISALFEAEKRGFHVIPNAEAVERALERSGFRAWDLASKELNIKTAKFSYVFNVDELEIKSSEIGFPLLIKPLMSSSGKGQSLVNRKEDLLLAWNDALKNSRGKIVGVILEEFLNFDYEFTLLTIRKTSGENIFCEPIGHEQYKGDYQCSWQPLDMNQSLINEARKMTTKILNNLNGSGIYGVEFFVRGKEVIFSELSPRPHDTGMVTLVSQNINEFELHLRAFLGMEKVEVVVHPQSIVHSMVEFVDGSVMAQCGASAVIYGGVEAQGVAFENVDQALAVPGTDIRLFGKPEAFERRRMGVAVAHGDDIEDCRARAREAAGRAARLVVLVSGSGTNLQVLLDACADPAFGAEVVAVGADRAGTRGVQRAAERGVPTFEAVVRDYASRDEWDAALTAMCREHAPDLIVSAGFLKLVGPAFLAEYGGRYVNTHNALLPAFPGMHGPRDALAYGVKVTGATLHFVDAGVDTGPIIAQVTVPVLDDDTEETLTERIKEAERQQLVGLVDLARGLLAHRYELIASGGTARALAEAGLPVTEVADVTGSPEVLSGRVKTLHPAIHAGILSRRTPADRTELASQGFRPIDLVIVNLYPFEDTVARQGVTEAEAIEQIDIGGVG",
    "response#2": "HEELGIRTAKYGYAITLEELIAVSDEIGFPNVVKPVMSSSGKGQSVVATKEEVEKAWNYAIANSRGDSQKVIVEEFINFEIEITLLTIKQWDSPTIFCSPIGHRQERGDYQESWQPAGISEDKILEAQAIAKKVTDALGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEELVSRDLKIKTANYDYIYKFEDLEKKADEIGFPLLLKPLMSSSGKGQSLVGSKNDLNQAWSQAQANSRGQVKGVIIEEFINFDFEFTLLTVRKNNGENIFCLPIGHLQSNGDYQCSWHPLEINQSLISEAKKMTTKILNNLNGSGLYGVEFFVKENEVIFSELSPRPHDTGMVTLVSQNINEFELHLRAFLKETGIDIHEELGIRTAKYGYAITLEELIAVSDEIGFPNVVKPVMSSSGKGQSVVATKEEVEKAWNYAIANSRGDSQKVIVEEFINFEIEITLLTIKQWDSPTIFCSPIGHRQERGDYQESWQPAGISEDKILEAQAIAKKVTDALGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEKKYLDKKKDLNELVSRDLKIKTANYDYIYKFEDLEKKADEIGFPLLLKPLMSSSGKGQSLVGSKNDLNQAWSQAQANSRGQVKGVIIEEFINFDFEFTLLTVRKNNGENIFCLPIGHLQSNGDYQCSWHPLEINQSLISEAKKMTTKILNNLNGSGLYGVEFFVKENEVIFSELSPRPHDTGMVTLVSQNINEFELHLRAFSELGKFKDLDDKI",
    "response#3": "ELAHEELGIRTAKYGYAITLEELIAVSDEIGFPNVVKPVMSSSGKGQSVVATKEEVEKAWNYAIANSRGDSQKVIVEEFINFEIEITLLTIKQWDSPTIFCSPIGHRQERGDYQESWQPAGISEDKILEAQAIAKKVTDALGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELHLRAIKQALKQKRELGELAHEELGIRTAKYGYAITLEELIAVSDEIGFPNVVKPVMSSSGKGQSVVATKEEVEKAWNYAIANSRGDSQKVIVEEFINFEIEITLLTIKQWDSPTIFCSPIGHRQERGDYQESWQPAGISEDKILEAQAIAKKVTDALGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELHLRAIGKARTVFWNGPMGVFEMPNFRKGTEAVARALEKLGVKKRYTLAGGGDTLAAINRYGIKVDVSHISTGGGALLEFLEGKELPGVAILNSKSK"
  },
  {
    "reference": "MTQTYTVAVVGATGAVGEVMLSILHERGFPIKELIPLASARSAGEKIDFGNQKIVVRDLATFDPKGVDIALFSAGGSISKEYGPKFAAAGAIVIDNSSAFRYDDDVPLVVSEVNPDALKNIPRGIVANPNCSTMQMVVALAPIHRAVGIERINVATYQSVSGGGRSGMEELGKQTAAILGFQEPDPQRFPVQIAFNLIPHIDEFMDNGYTKEEMKLVWETRKILGDENIQVNPTAVRVPVFFGHSEAVHIETRNKISVAEARALLEKAPGVVVVDDRVPGGYPTPVTHASGQDPVFVGRLREDVSHPRGLDMWVVSDNIRKGAALNAVQLAELVLKNRA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that is capable of catalyzing reactions within the Amino-acid biosynthesis pathway with high efficiency and specificity.2. The designed protein must have a Proton acceptor, Acyl-thioester intermediate active site that is highly conserved among related enzymes.3. The designed protein must possess NAD binding, protein dimerization activity to support threonine biosynthetic process under certain conditions.4. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate. The designed protein sequence is ",
    "response#1": "LKPLHDKYRIKRVVVSTYQSVTGTGVKAVEQMMNERKGVMDGPMAYAYPIDLNVIPQIDVFTENGYTKEEMKMILETRKIMGDDSIRVTATTVRIPVMGGHSESVNIEFENDFDVAEVKALLERTEGIIVVDDPANLKYPMPMDAHEKDEVFVGRIRRDESLPNTLNMWIVADNLRL",
    "response#2": "PLHKAAGLRRMIVSTYQAASGAGMPGLRELQRETSVLANGGEMGDTSPFAYQLAANLIPQIGSFKDEGYTSEEWKMQNEGRKIMHLPEVRVNCTCVRVPVMRSHSESITCEFDRPLTPDQAREILAAAPGVKVVDDPATLSYPMPLDTSDQDLVYVGRIRRDLSAPEGVSSLTFWCCGDQIRQ",
    "response#3": "LGGLFRENLVEWATSMTYQAASGAGAQNMRELLQQMGEAHRVAGDLLRDPASAILDIDREVAGTLRDERFPTAHFGVPLAGSLIPWIDKDLGNGQSKEEWKGGAETNKILGREANPTPIDGLCVRIGAMRCHSQALTIKLTRDVPLDEIDAIIASANSWVKVVPNEREASMHELTPTAVTGTLAVPVGRLRKMNMGSEYLSAFTVGDQLLVL"
  },
  {
    "reference": "MLIGTFTPKLDDKGRLILPAKFRERFATGLVLTRGQENCVFVFPRDEFLQVHENLRRAPLTSKQSRDFNRVLLAGAHDELPDKQGRVTIPPILREWARLDRDLTVIGTGSKLEVWDTGTWQGYFADAAASFDDAVPEVVPGLG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must have at least one SpoVT-AbrB domain.2. The protein should have DNA-binding transcription factor activity and be active in nucleoid. The designed protein sequence is ",
    "response#1": "MFRGINAITIDTKGRLAIPTRYRSALGAEDKIPLVVTIDTEETCLLLYTAAQWQIIEDNLQKLPSFNAAARRIQF",
    "response#2": "EAAATDEQHPDAQGRITLSADHRRYADLSKDCVVIGSVDYLEIWDAAKWQQYLEEHEENFSTASDL",
    "response#3": "SFFSGATECELDNQGRIRIPNNLRSHAYLEKEVIVIGVATRIEIWSSDQWGQYNDDSNLSYDEIANRM"
  },
  {
    "reference": "MDINHQNQCIPYNCLSNPDAILLDAERLETGNTVADISLGLINFLYSNFVPGGGFIVGLLELIWGFVGPSQWEIFLAQIEQLISQRIEEFARNQAISRLEGLSNNYEIYTETFRAWEKDPSNPALREEMRTQFNVMNSALIAAIPLLRVRNYEVALLSVYVQAANLHLSVLRDVSVYGQRWGFDPATVNSRYSDLTRLIHVYTDHCVDTYNDGLKNLEGSRLSDWVVYNRFRRRLTISVLDIIAFFPNYDIEAYPIQTASQLTREVYLDLPFVNETLSPPASYPTFSAAESAIIRSPHLVDFLNSFTIYTDSLASYAYWGGHLVNSFRTGTTTNLIRSPLYGREGNTERPVTISASPSVPIFRTLSYFTGLNNNNPVAGIEGVEFQNTISRSIYRKSGPIDSFSELPPQDVSVSPAIGYSHRLCHATFLERISGPRIAGTVFSWTHRSASPINEVSPSRITQIPWVKAHTLASGASVIKGPGFTGGDILTRNSMGDLGALRVTFTGRLPQSYYIRFRYASVANRSGTFRYSQPPSYGISFPKTMDAGEALTSRSFAHTTLFTPITFSRAQEEFDLYIQSGVYIDRIEFIPVDATFESEINLERAQKAVNALFTSTNQLGLKTDVTDYHIDQVSNLVECLSDEFCLDEKRELSEKVKHAKRLSDERNLLQDPNFRGINRQPDRGWRGSTDITIQGGDDVFKENYVTLTGTFDECYPTYLYQKIDESKLKAYTRYQLRGYIEDSQDLEIYLIRYNAKHEIVNVPGTGSLW",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Promotes colloidosmotic lysis by binding to the midgut epithelial cells of insects..2. The protein design must be able to perform toxin activity, signaling receptor binding in order to contribute to cytolysis by symbiont of host cells. The designed protein sequence is ",
    "response#1": "TDFQLSRVIYTDPIGFVHRSSLRGESWFSFVNRANFSDLENAIPNPRPSWFLNNMIISTGSLTLPVSPSTDRARVWYGSRDRISPANSQFITELISGQHTTATQTILGRNIFRVDSQACNLNDTTYGVNRAVFYHDASEGSQRSVYEGYIRTTGIDNPRVQNINTYLPGENSDIPTPEDYTHILSTTINLTGGLRQVASNRRSSLVMYGWTHKSI",
    "response#2": "ITQIPLTKVDTRGTGVSYVNDPGFIGGALLQRTDHGSLGVLRVQFPLHLRQQYRIRVRYASTTNIRLSVNGSFGTISQNLPSTMRLGEDLRYGSFAIREFNTSIRPTASPDQIRLTIEPSFIRQEVYVDRIEFIPVP",
    "response#3": "KGFPAEKGYGDSIAYVSEPLNGANAVKLTSYQVLQMEVTNQTTQKYRIRIRYATGGDTAASIWFHIIGPSGNDLTNEGHNFSSVSSRNKMFVQGNNGKYVLNILTDSIELPSGQQTILIQNTNSQDLFLDRIEFK"
  },
  {
    "reference": "MVAKIQADEISSIIKERIDNFELSVDINETGKIVSYADGVAQVYGLSNVMAGEMVEFEEGTRGLVMNLEESTVGVVILGPGPLLKEGMSVKRLGRLLRVPVGDALLGRVVNALGEPIDGKGPIETTETRFVEEKAPGIMARKSVHEPLATGIKAIDALVPIGRGQRELIIGDRQTGKTTVALDAIINQKGNGVVCIYVAVGQKESTVAQIIRRLEDHGAMEYTIVVSSTAAEAAALQFLAPYTGVTMGEYFRDNARHGLIVYDDLSKHAVAYREMSLILRRPPGREAYPGDVFYIHSRLLERAAKVSDERGAGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLETELFNSGVRPAINVGLSVSRVGGAAQIKATKQVAGTLRLDLAQYRELQAFAQFASDLDETSRKQLERGQRMVEVLKQPPFSPLSVEKQVLIIFAGNEGFFDDMAPSNVVRFEAELYPFIEASYPQIFENIRSTSKVDDNTNALMKKALEEFKASFIVA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. We are looking for a protein with broad substrate specificity for ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate.2. The protein should be able to bind ATP ligand in a variety of conditions3. The protein must exhibit the following characteristics: The alpha chain is a regulatory subunit.; Produces ATP from ADP in the presence of a proton gradient across the membrane.4. The protein can be expressed in proton-transporting ATP synthase complex, catalytic core F(1), plasma membrane and perform proton-transporting ATP synthase activity, rotational mechanism, proton-transporting ATPase activity, rotational mechanism, ATP binding without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "GVKVIDLICPFAKGGKVGLFGGAGVGKTVNMMELIRNIAIEHSGYSVFAGVGERTREGNDFYYEMEESGVLDKVALVYGQMNEPPGNRLRVALTGLTMAEYFRDEGRDILFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLSQEMGQIQERITSTKTGSITSIQAVYVPADDLTDPAPATTFAHLDATVVLNRSIAETGIYPAIDPLDSTSRMLSPHQIKAMKQVAGSLRLDLAQYRELAAFAQFGSDLDKATLARLTRGERLVELLKQDQYKPMPVEEQVMAIFAAVNGYLDDLPVEKVRPFEAEFLKFMRANYPQIGEEIRTKGVLTDELTSRLKSAIEEYDHLPEQAFYMVGSIEEAVEKANKM",
    "response#2": "GIKVVDLLAPYIIGGKIGLFGGAGVGKTVLIQELINNIAQEHGVISVFAGVGERTREGNDLYHEMTDSGVIKKSAMVFGQMNEPPGARMAVALSGLTMAEHFRDRDGQDVLLFVDNISFTQAGSEVSALLGRMPSAVGYQPTLATEMGQLQERITSTKVGSVTSIQAIYVPADDYTDPAPATTFAHLDATTNLERKLSEMGIYPAVDPLASTSRMLSPHQLPIMKQVAGTLKLELAQFAELEGFSQFSSDLDQASQNQLARGQRLREILKQSQASPLSVPDQVASIYAGTNGFLQKLAVNQVRDFLVGLREFLDKRKPEYLEAVNSEKKLTPKVKDILNSAISEEFDHIPEQAFYMVGGIEEVIENAKKLAA",
    "response#3": "MKKVAGTLKIDQAQFRELESFSKFGGEMDAVTAFTIDKGQKNTQLLIQPQYSPMPVEEQISILYCGTQGLLKEVPLDKVHEFEARFLQELRTSHQRDVLDVLKTGVINDEIRGILEKTAKEGIKVIDLLAPLAQGGKAAMFGGAGVGKTVFVMELIHAMVERYRGISVFAGIGERSREGHEMLLDMRGSGVLGRTVLVYGQMNEPPGARWRVPLTALAIAEYFRDERAQNVLLLMDNVFRFVQAGAEVSGLLGRLPSRVGYQPTLASEVAALQERIASVEGAAVTAIEAVYVPADDFTDPAVTAIAAHVDSMVVLSRAMAAEGMYPAIDPVASSL"
  },
  {
    "reference": "MELIPKRSLPEAEHKREFVRTIFDRIAPRYDLLNRIITLGLDQSWRRAALDSVGLKPGERVVDLATGTGDLAELACARGAQAIGVDFSGGMLRAARNRRPHGLFVQADVSQLPFRDASAGVVTCGFALRNFTDLNTVFAECARILEPGGRLVLLEVDEPRSRLLRWGHALHFQHLVPRLGAWLSDREAYRYLPASATYLPSEEELIARLEAAGFTRVSKRRHLFGAVQQIRATRI",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein with 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity, demethylmenaquinone methyltransferase activity, S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity, S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity functions is required to enable aerobic respiration, methylation.2. We need a protein that can perform a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine with high efficiency.3. The protein should have a high affinity for S-adenosyl-L-methionine.4. A protein should be able to manipulate the activity of enzymes in the Quinol/quinone metabolism, menaquinone biosynthesis, menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2 pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "MSRSSFGYRDVPAAEKSARVGAVFSNVAARYDLMNDLMSAGLHRWWKDRFVRRVRPRAGEQILDMAGGTGDIAFRMARRGASITVADINAEMLAVGRDRAAARQVDNIDFRCANAEDLPFPDRCFDAYTIAFGIRNVTDIPAALREAHRVLRPGGILLILEFSTTLWPGFAEVYDAYSHRLVPRLGQLIAGDEDSYRYLIESIRRFPDMPTFERMIEAAGFARTRVEPILGGLVAIHSGWKI",
    "response#2": "MSKTLEEAIEDIRQGKMVIIVDDEDRENEGDLICAAQFATPDAINFMAKYGRGLICLTLTEDRADELELPLMVPENTSRFETAFTVSIEAKQGVTTGISAADRAHTILTAIADNASPADLARPGHVFPLRAKEGGVLKRAGHTEAAVDLARLAGLYPAGVICEIMNEDGTMARVPDLEIFAKKHGLKIITVADLIRYRRQHEKLVQQGA",
    "response#3": "MSFDLIIQNGKVVNPKSGEDFVADVLIDGNTIAQISEEIDADKIIDASGK"
  },
  {
    "reference": "MTAENNHYGSRLLHLRLPRVCVAIAGSDANEMLEKAEALARDNPFIELRLDYLPKPGLAPEKIREFTESHPHVAVIGTCRRLASGGKFRGPISSQLDLLGKVADAGCQLVDVEIQTAARMRSAQLDKLRGKAAVVLSFHDFRGTQKLDETLNKMTAFPADFYKIVSTATSLFDNVSMMKFLERHQDAHSLVGLCMGEQGIISRVLAARAGSAFTFGALSADEKTAPGQVTARELRSIYRIDQVDAATKVYGVAGDPIEHSLSPAIMNAALRRENVNGVFLALHAKSLKDLLACVKEIPIHGLAITMPHKQAILPHLDNTDPFTTKIGACNTIVRSADGKLYGFNTDTSGVVRPLEQRIGLQNAKILVLGAGGAARAAVFGLKDRGAQVFILNRHTEKGKKLARQAKARTMKRLDLKKLDFDVIINATSVGMGNTRESPLSADEIKAKYVFDMVYDPAETLLLKLAKERGCEVIAGSEMFVHQAARQFEIWTGKPAPREDMLKIVTQALAERAAARNGTKLISKAS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be able to bind shikimate ligand in a variety of conditions2. The designed protein should contain one or more SDH C-terminal, Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase domains that are essential for its function.3. The protein must exhibit the following characteristics: Catalyzes the cis- dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate..4. A protein with 3-dehydroquinate dehydratase activity functions is required to enable amino acid biosynthetic process. The designed protein sequence is ",
    "response#1": "GMAEVVKTAAIWNEDEFMSLESRSAEIFAAIQTPSANFAGRSKATRSTAQELLLSVIVGSISVKAHIVTIDERETGLRNLVNFGHTIGHAIEAILTPTILHGECVSVGMILEGEISRQMGILSQVGVGRLTRCLKAYNLPVTLTDPRIASLPAAKQLTVDRLLDIMRIDKKV",
    "response#2": "GMAEVVKTAAIWNEDEFMSLESRSAEIFAAIQTPSANFAGRSKATRSTAQELLLSVIVGSISVKAHIVTIDERETGLRNLVNFGHTIGHAIEAILTPTILHGECVSVGMILEGEISRQMGILSQVGVGRLTRCLKAYNLPVTLTDPRIASLPAAKQLTVDRLLDIMRIDKKL",
    "response#3": "GMAEVVKTAAIWDEAEFTTLEENAPAILHAVRSRSDASAGRLDSIKHILKRIVLGSARVKAEVVSSDEREGGLRNLLNFGHSIGHAIEAILTPQVLHGEAVAIGMVKEAELARFLGILSPGAVARLVKCIASYELPTSLRDKRIVKLTAGKECPVDVLLEKMAVDKKGMAEVVKTAAIWNEDEFMSLESRSAEIFAAIQTPSANFAGRSKATRSTAQELLLSVIVGSISVKAHIVTIDERETGLRNLVNFGHTIGHAIEAILTPTILHGECVSVGMILEGEISRQMGILSQVGVGRLTRCLKAYNLPVTLTDPRIASLPAAKQLTVDRLLDIMRIDKKG"
  },
  {
    "reference": "MGGKWSKSSKEGWSQVREKMKQTPVAAEGAAEGVGAVSQDLAKHGAVTSSNMNNASRVWLAAQEDDDDNDVGFPVRPQVPLRPMTYKGAFDIGFFLKEKGGLDGLIWSQKRQDILDLWVYNTQGFFPDWQNYTPGPGIRYPLCFGWCYKLVPVDPREVEENNTGENRCLLHPMSQHGIEDAEREVLKWEFDSSLARRHIARELHPEYYKDC",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein's functional design necessitates the inclusion of a Dileucine internalization motif; necessary for CD4 internalization motif.2. The designed protein should have SH3 domain binding, GTP binding within extracellular region, host cell plasma membrane, host cell Golgi membrane to regulate suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I.3. The protein must exhibit the following characteristics: Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis. The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPEVRERMRRAEPAAEGVGAASQDLDKHGALTTSNTVSNNAACAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQQRQDILDLWVYNTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPEKIEEANAGENNSLLHPMSLHGMEDEEREVLVWKFDSRLAFHHMARELHPEYYKN",
    "response#2": "MGGKWSKSSIVGWPEIRERIRRAEPAAEGVGAASQDLDKYGALTTSNTPTNNAACAWLEAQEEEEVGFPVRPQVPLRPMTFKGAFDLGFFLKEKGGLDGLIYSKKRQEILDLWVYNTQGFFPDWQNYTPGPGIRYPLTFGWCYKLVPVDPREVEEANEGENNCLLHPISQHGMEDEEREVLMWKFDSSLARRHIARELHPEYYKDC",
    "response#3": "MGGKWSKSSIVGWPEIRERIRRAAPAAEGVGAASQDLDKYGALTSSNTAHNNPDCAWLQAQEEEDEVGFPVRPQVPLRPMTFKGAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYNTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPREVEEANEGENNCLLHPMSQHGMEDEDREVLKWEFDSSLARRHLAREKHPEFYKDC"
  },
  {
    "reference": "MSESARTDVVLVGAGIMSATLGAMLRRLEPDWSITLIERLDAVAAESSGPWNNAGTGHSALCELNYTPQRPDGSIDISKAVRINEEFQVTRQFWAYAAENGILNDVRSFLNPLPHVSFVHGAERVHQLRRRRDALAGNPLFAGAEWIDDPDEFARRLPLMAAKRVFSEPIALNWAADGTDVDFGALSRQLIGYCVRNGAVALFGQEVRNLVRQPDGGWMLTVVNRRTGEKRKLKTKFVFVGAGGNALSLLQRSGIKEINGFAGFPIGGRFLRTANPALTAAHRAKVYGVPAPGAPPLGALHLDLRYVNGKSWLMFGPFAGWSPKFLKRGHLRDLPGSIKPNNILSMVGVGVTQVTLLNYLIGQLRLSEPDRVAVLRKFVPSAADSDWELIVAGQRVQVIRRDKRKGGVLEFGTTVVGEADGSIAGLLGGSPGASTAVPIMLEVLQRCFTHRYQSWLPALKEMVPSLGANLSEEPVLYDEVRSWSSRALQLDAS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein with malate dehydrogenase (quinone) activity, malate dehydrogenase (menaquinone) activity is needed to enhance tricarboxylic acid cycle in a specific context.2. The stability of the protein-FAD complex should be optimized to maximize enzymatic activity.3. The protein design should consider the endogenous proteins and enzymes present in the oxaloacetate from (S)-malate (quinone route): step 1/1, Carbohydrate metabolism pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "LDSARFAAFIAWELGVSVKDVTAVTLGGHGDDMVPLVRYTSVCGVPVMELLEQKYGAAKAAEVMAAMVKRTRGAGGEVVALLKTGSAFYSPASSAIAMAESFLKDQKRVLPTCAFLKGEFGVDGLYVGVPVVIGAGGAERVLQLKLNAEEQAMMDKSVKAVKLA",
    "response#2": "LDSARFAAFIAWELGVSVKDVTAVTLGGHGDDMVPLVRYTSVCGVPVMELLEQKYGAAKAAEVMAAMVKRTRGAGGEVVALLKTGSAFYSPASSAIAMAESFLKDQKRVLPTCAFLKGEFGVDGLYVGVPVVIGAGGAERVLQLKLNAEEQAMMDKSVKAVKQ",
    "response#3": "LDSARFAAFIAWELGVSVKDVTAVTLGGHGDDMVPLVRYTSVCGVPVMELLEQKYGAAKAAEVMAAMVKRTRGAGGEVVALLKTGSAFYSPASSAIAMAESFLKDQKRVLPTCAFLKGEFGVDGLYVGVPVVIGAGGAERVLQLKLNAEEQAMMDKSVKAVKAA"
  },
  {
    "reference": "MSEITLGKYLFERLKQVNVNTIFGLPGDFNLSLLDKIYEVDGLRWAGNANELNAAYAADGYARIKGLSVLVTTFGVGELSALNGIAGSYAEHVGVLHVVGVPSISAQAKQLLLHHTLGNGDFTVFHRMSANISETTSMITDIATAPSEIDRLIRTTFITQRPSYLGLPANLVDLKVPGSLLEKPIDLSLKPNDPEAEKEVIDTVLELIQNSKNPVILSDACASRHNVKKETQKLIDLTQFPAFVTPLGKGSIDEQHPRYGGVYVGTLSKQDVKQAVESADLILSVGALLSDFNTGSFSYSYKTKNVVEFHSDYVKVKNATFLGVQMKFALQNLLKVIPDVVKGYKSVPVPTKTPANKGVPASTPLKQEWLWNELSKFLQEGDVIISETGTSAFGINQTIFPKDAYGISQVLWGSIGFTTGATLGAAFAAEEIDPNKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYTIEKLIHGPHAEYNEIQTWDHLALLPAFGAKKYENHKIATTGEWDALTTDSEFQKNSVIRLIELKLPVFDAPESLIKQAQLTAATNAKQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: In a side-reaction the carbanionic intermediate (or active aldehyde) generated by decarboxylation or by activation of an aldehyde can react with an aldehyde via condensation (or carboligation) yielding a 2- hydroxy ketone, collectively called acyloins; The expression level of this protein in the presence of fermentable carbon sources is so low that it cannot compensate for the other two pyruvate decarboxylases to sustain fermentation.; Minor of three pyruvate decarboxylases (PDC1, PDC5, PDC6) implicated in the nonoxidative conversion of pyruvate to acetaldehyde and carbon dioxide during alcoholic fermentation; Most of the produced acetaldehyde is subsequently reduced to ethanol, but some is required for cytosolic acetyl-CoA production for biosynthetic pathways.2. The designed protein should have a binding site that is specific to the target pyruvate, Mg(2+) ligand.3. The protein must be engineered to have a cooperative binding mechanism with thiamine diphosphate to ensure proper regulation.4. Ideal protein should have a binding affinity for key enzymes in the Ehrlich pathway, Amino-acid degradation pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MRRMVYTRILDQRSISLNRQGRLGFYAPTAGQEASQIASHFALEKEDFILPGYRDVPQIIWHGLPLYQAFLFSRGHFHGNQIPEGVNVLPPQIIIGAQYIQAAGVALGLKMRGKKAVAITYTGDGGTSQGDFYEGINFAGAFKAPAIFVVQNNRFAISTPVEKQTVAKTLAQKAVAAGIPGIQVDGMDPLAVYAAVKAARERAINGEGPTLIETLCFRYGPHTMSGDDPTRYRSKELENEWAKKDPLVRFRKFLEAKGLWSEEEENNVIEQAKEEIKEAIKKADETPKQKLVTPEDLPFIIADVSGRTGEKVLTIKSCNIHSGIGIRPHAQIELEYQGKIHKEISEGDGGYDAFMNALTKITNRLGISIPKLIDYEVRIPPGGKTDALVETRITWNKSLDLEEDQTFKTMGVHPDQTVAAVHATEKMLNQIL",
    "response#2": "MRRMVYTRILDQRSISLNRQGRLGFYAPTAGQEASQIASHFALEKEDFILPGYRDVPQIIWHGLPLYQAFLFSRGHFHGNQIPEGVNVLPPQIIIGAQYIQAAGVALGLKMRGKKAVAITYTGDGGTSQGDFYEGINFAGAFKAPAIFVVQNNRFAISTPVEKQTVAKTLAQKAVAAGIPGIQVDGMDPLAVYAAVKAARERAINGEGPTLIETLCFRYGPHTMSGDDPTRYRSKELENEWAKKDPLVRFRKFLEAKGLWSEEEENNVIEQAKEEIKEAIKKADETPKQKLVTPEDLPFIIADVSGRTGEKVLTIKSCNIHSGIGIRPHAQIELEYQGKIHKEISEGDGGYDAFMNALTKITNRLGISIPKLIDYEVRIPPGGKTDALVETRITWNKSLDLEEDQTFKTMGVHPDQTVAAVHATEKMLNQIA",
    "response#3": "MRRMVYTRILDQRSISLNRQGRLGFYAPTAGQEASQIASHFALEKEDFILPGYRDVPQIIWHGLPLYQAFLFSRGHFHGNQIPEGVNVLPPQIIIGAQYIQAAGVALGLKMRGKKAVAITYTGDGGTSQGDFYEGINFAGAFKAPAIFVVQNNRFAISTPVEKQTVAKTLAQKAVAAGIPGIQVDGMDPLAVYAAVKAARERAINGEGPTLIETLCFRYGPHTMSGDDPTRYRSKELENEWAKKDPLVRFRKFLEAKGLWSEEEENNVIEQAKEEIKEAIKKADETPKQKEITDWDLEAIVKDETQQPPELFRLELVQVSCGDHAQPTATITIRTPGGKELTDAAIGTGPVDAIYKAINRVVQVPNELIEYSVQSVTAGIDAIGEVTIRLRHEGRIYSGHAANTDIVVASARAYISALNRLLE"
  },
  {
    "reference": "MAQHRKLGLPSDHRRAMLRNLVTSFLKHGKIQTTVTRAKEARSLAEKMITLAKRGDLHARRQVLSFVTEEEVVKNLFTNIAPKYAERNGGYTRMYKIGPRRGDGAELVILELV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that contributes to translation by functioning in ribosome, ribonucleoprotein complex with structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "RRLDAVVYRAKFVSTMFAARQFINHGHIKVNGKRVNIPSYKVRVGDVIEVKEASKQLAFVLEASI",
    "response#2": "LESRLDNLVYRLGLARTRRQARQLG",
    "response#3": "LESRLDNLVYRLGLARTRRQARQLK"
  },
  {
    "reference": "MKSSAKLMYGPTVFMAAMAVIYIFATMHVSDGGSVKGVEWVGSVALVLSAGLTLMLGVYLHFTEVRVDVLPEDWEEAEVADKAGTLGFFSPSSIWPAAMSGAVGFLAFGVVYFHYWMIAVGLMLLIFTITKLNLQYGVPKEKH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be expressed specifically in the plasma membrane The designed protein sequence is ",
    "response#1": "AFHDHILMPIITAITLLVLGLLIWIVIRYNAKANPVPAKFSHNTTVEIIWTIGPVLILMVIAIFSFRLLFAYNDMPKPYLTVKATGYQWYWGYEYPDQKISEITSVPLKQEEAVAKGVPYLLAADKPMVVPVNQVIRVQVTGADVIHAFALPAFGLKTDAIPGRLNETWFKADKIGVYYGQCSELCGVDHSNMPIEIHVVSQADFDAWM",
    "response#2": "AFHDHILMPIITAITLLVLGLLIWIVIRYNAKANPVPAKFSHNTTVEIIWTIGPVLILMVIAIFSFRLLFAYNDMPKPYLTVKATGYQWYWGYEYPDQKISEITSVPLKQEEAVAKGVPYLLAADKPMVVPVNQVIRVQVTGADVIHAFALPAFGLKTDAIPGRLNETWFKADKIGVYYGQCSELCGVDHSNMPIEIHVVSQADFDAWQ",
    "response#3": "LTVKATGYQWYWGYEYPDQKISEITSVPLKQEEAVAKGVPYLLAADKPMVVPVNQVIRVQVTGADVIHAFALPAFGLKTDAIPGRLNETWFKADKIGVYYGQCSELCGVDHSNMPIEIHC"
  },
  {
    "reference": "MAGKGKIIGIDLGNETNSVVAVMMGDEAVVIQNQEGSRLTPSVVSWTKEKEVLVGDPAKRRAILDPENTVYESKRFIGRKFEEVKEEAKRVSYKVVPDEKGDAAFDIPNAGKLVRPEEVGAHVLRKLKEAAEAFLGEPVKKAVITVPAYFNERQRQATKDAGKIAGLEVVRILNEPTRAAMAYGLHKKENVRILVYDFGGGTFDVSILEGGDGVIEVKATAGDTHLGGANIDERIMEWLIEEFKKEHGIDLRQDRTALQRLKEAAEQAKKELSFKMETEINLPFITIDPNTNQPLHLQKKLTRARLEEMINDLIDRTIDIVKQALEDANVKPSDIDEVVLVGGSTRIPLVQQKIKEFFGKEPHKGLNPDEVVAMGAAIQAGVLAGEVKEIVLVDVTPLELGVETYGGVMTVLIPRNTPIPVKKSKVFTTAHDYQTEVEIHVLHGERPLAKDNKSLAKFYLTGIPPAPRGVPKIEVCFDIDADGILHVTAKDLGTGKEQSVRVEISSGLTPEEIERIIKEAEETREEDRKKKELIEAKNQLDHLVYQLEKTLKEAGDKVPADVKQEAEKVIEEAKKTIETATDINEAKELQKEFSRYPQSSEQPFT",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Acts as a chaperone..2. The designed protein must be able to perform ATP-dependent protein folding chaperone, unfolded protein binding under specific conditions. The designed protein sequence is ",
    "response#1": "QKLLDMVINSIYSQREVFLRELISNASDAIDKIYYKALTDDSLSFDVDNYYIKITPNKEERTLTVVDTGIGMTKEELENNLGVIAKSGSHTFKSENEIKDGHDIIGQFGVGFYAAFMVADKVEVVTKSIDSDAAFKWESKGSDGYSITEADKQDIGTTITLYIKENQEEENYDEFLDTFTLQQIIKKYSDFF",
    "response#2": "QKLLDMVINSIYSQREVFLRELISNASDAIDKIYYKALTDDSLSFDVDNYYIKITPNKEERTLTVVDTGIGMTKEELENNLGVIAKSGSHTFKSENEIKDGHDIIGQFGVGFYAAFMVADKVEVVTKSIDSDAAFKWESKGSDGYSITEADKQDIGTTITLYIKENQEEENYDEFLDTFTLQQIIKKYSDFN",
    "response#3": "QKLLDMVINSIYSQREVFLRELISNASDAIDKIYYKALTDDSLSFDVDNYYIKITPNKEERTLTVVDTGIGMTKEELENNLGVIAKSGSHTFKSENEIKDGHDIIGQFGVGFYAAFMVADKVEVVTKSIDSDAAFKWESKGSDGYSITEADKQDIGTTITLYIKENQEEENYDEFLDTFTLQQIIKKYSDFS"
  },
  {
    "reference": "MESYSVKVGSSSQSPEGSAAGAAAETLTSPTVGQAVISSNCSTTQAGNFPHLVRKNLAHVEAVEATATAAESVVPMQSRSLSLVTGTSKSAPASTGVFAGDMDWPQWGLVSVVSLVVTPASGSSAAAEMNTAPTLEMLGRISVLSTDNATVSTLSPPSCDPIASCHDFYRRLNSSLGVSSASSVPLLRSIPPPPATSLGDSSSCSDDSAAAATAVSPLSTLLSRRCSGQHPPSLLNNVVARLNPSLLLMAQTLCGCPFAEVMCPVLVMESSRARIAAAAIRNPDPASSSAMTVDPDTQRQGLPLTLGRPPHSGSSSSSLRHLDSPQPLASNAAVLDRKEDDMAEDSRGTYHQAAAAVANGDASDAGVDDEGGPETNSAPASRILKSISKTLMGRASSSSHNTTTNAVTMATSTAATATGTFTPNHSALPLEKLNSDSPTPPPPCAWSLVYANDAAASMLGCSSVDEVTTAAFDNLLCCFALTLPPSASAGSIRSSMPLVTAAAATDGESVPFALASLPATASSSSPQAACFGEKEMASNDENDDKGRQKHQQPSSNAVQQPGWREVQNPGELFALPNHNYVILYSKRTSAYYAALAVPSHQPKMSQRTSVDGESTPRMGSPSTMRNQSLCPTTQQAECYPPVPRNPSHPSADVPSSRLTGSEEATGMAQCGRRTSTILAAANTSSPLEPDLSDGASPPPPMQQQRCSRYVEIYILQRLESSHRPAGIARMPTARAVAAGPPPTLVTLGSGAADARGPGARTTTSPGDH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have iron ion binding, ATP binding, acireductone dioxygenase (Ni2+-requiring) activity, nickel cation binding and be active in cytoplasm, nucleus.2. The protein must be engineered to have a high affinity for Fe(2+), Ni(2+) in order to increase its catalytic activity.3. For general function, the protein need meet that Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.4. The Fe(2+) binding site should be located in a region of the protein that is accessible to the ligand.5. The protein design should emphasize Basic and acidic residues, Polar residues, Pro residues compositional bias.6. The protein design should take into account the physiological conditions of the L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6 pathway. The designed protein sequence is ",
    "response#1": "LCPRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGGLLGVRDEMVLGVKDCAGPRSAAEMSEAEREALGIRERMPLGCEEGRNALEGDTELKGVLGEEFVERYLAVNRTLPRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGGLLGVRDEMVLGVKDCAGPRSAAEMSEAEREALGIRERMPLGCEEGRNALEGDTELKGVLGEEFVERYLAVNRTLETVLVDKKETEEQRLTRWLRSMRAYYFDNLPGDQRAPHDYVPSRPVDASALAKMNVLSWHL",
    "response#2": "LCPRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGGLLGVRDEMVLGVKDCAGPRSAAEMSEAEREALGIRERMPLGCEEGRNALEGDTELKGVLGEEFVERYLAVNRTLGPRWNPKLLPRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGGLLGVRDEMVLGVKDCAGPRSAAEMSEAEREALGIRERMPLGCEEGRNALEGDTELKGVLGEEFVERYLAVNRTLETVLVDKKETEEQRLTRWLRSMRAYYFDNLPGDQRAPHDYVPSRPVDASALAKMNVLSWHPRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGGLLGVRDEMVLGVKDCAGPRSAAEMSEAEREALGIRERMPLGCEEGRNALEGDTELKGVLGEEFVERYLAVNRTLETVLVDKKETEEQRLTRWLRSMRAYYFDNLPGDQRAPHDYVPSRPVDASALAKMNVLSWH",
    "response#3": "PRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGGLLGVRDEMVLGVKDCAGPRSAAEMSEAEREALGIRERMPLGCEEGRNALEGDTELKGVLGEEFVERYLAVNRTLETVLVDKKETEEQRLTRWLRSMRAYYFDNLPGDQRAPHDYVPSRPVDASALAKMNVLSWHGRGSGGTAPRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGGLLGVRDEMVLGVKDCAGPRSAAEMSEAEREALGIRERMPLGCEEGRNALEGDTELKGVLGEEFVERYLAVNRTLETVLVDKKETEEQRLTRWLRSMRAYYFDNLPGDQRAPHDYVPSRPVDASALAKMNVLSWH"
  },
  {
    "reference": "MSDILRELLCVSEKAANIARACRQQEALFQLLIEEKKEGEKNKKFAVDFKTLADVLVQEVIKQNMENKFPGLEKNIFGEESNEFTNDWGEKITLRLCSTEEETAELLSKVLNGNKVASEALARVVHQDVAFTDPTLDSTEINVPQDILGIWVDPIDSTYQYIKGSADIKSNQGIFPCGLQCVTILIGVYDIQTGVPLMGVINQPFVSRDPNTLRWKGQCYWGLSYMGTNMHSLQLTISRRNGSETHTGNTGSEAAFSPSFSAVISTSEKETIKAALSRVCGDRIFGAAGAGYKSLCVVQGLVDIYIFSEDTTFKWDSCAAHAILRAMGGGIVDLKECLERNPETGLDLPQLVYHVENEGAAGVDRWANKGGLIAYRSRKRLETFLSLLVQNLAPAETHT",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The desired protein should be able to perform 1D-myo-inositol 1,3,4-trisphosphate + H2O = 1D-myo-inositol   3,4-bisphosphate + phosphate2. The designed protein should be optimized for metal ion binding, inositol-1,3,4-trisphosphate 1-phosphatase activity, inositol-1,4-bisphosphate 1-phosphatase activity activity in order to improve signal transduction.3. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.4. The protein should be able to bind Li(+), Mg(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "LEVITQLRFPVSDSERFGVPEQVVVMDSSVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNHGGVIFKVYVVGDHVKCVKRRSLPDISEEKIGTSKGSLPFSQISNLTAQEDKNIEYGEDRSLEKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKMPSYEPVLTEFFWDMVTKKNQ",
    "response#2": "MKDDRICSPPFMELTSLSGDDTMRLLEQNGLAFPFICKTRVAHGTNSHEMAIVFNQEGLSAIQPPCVVQNFINHNAVLYKVFVVGESYTVVQRPSLKNFSAGTSDRESIFFNSHNVSKPESSSVLTALDKIEGVFERPSDEVIRELSRALRQALGVSLFGIDIIINNQTGQHAVIDINAFPGYEGVSEFFTDLLNHVEAYMEDDRICSPPFMELTSLCGDDTMRLLEKNGLTFPFICKTRVAHGTNSHEMAIVFNQEGLNAIQPPCVVQNFINHNAVLYKVFVVGESYTVVQRPSLKNFSAGTSDRESIFFNSHNVSKPESSSVLTELDKIEGVFERPSDEVIRELSRALRQALGVSLFGIDIIINNQTGQHAVIDINAFPGYEGVSEFFTDLLNHIATVLQGQSLEVITQLRFPVSDSERFGVPEQVVVMDSSVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNHGGVIFKVYVVGDHVKCVKRRSLPDISEEKIGTSKGSLPFSQISNLTAQEDKNIEYGEDRSLEKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKMPSYEPVLTEFFWDMVTKKNLEVITQLRFPVSDSERFGVPEQVVVMDSSVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNHGGVIFKVYVVGDHVKCVKRRSLPDISEEKIGTSKGSLPFSQISNLTAQEDKNIEYGEDRSLEKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKMPSYEPVLTEFFWDMVTKKN",
    "response#3": "KSICCPPYLEINTSDPSVIQAAVRSRQLSYPLICKTRVAHGSRSHEMSLIFSEVRLCDVRSPCILQSFVNHGAVLHKVFVVGDDHFTVERPSLKNFPPGPCDRSTIFFNSHDVSKPESSSHLTALDDTMPRLPPPSSDAMVALVRQLRVELGMALFGVDVIVNIHTHTLTVIDINIFPGYEGVPQFFSSLLSYEAYMKDDRICSPPFMELTSLSGDDTMRLLEQNGLAFPFICKTRVAHGTNSHEMAIVFNQEGLSAIQPPCVVQNFINHNAVLYKVFVVGESYTVVQRPSLKNFSAGTSDRESIFFNSHNVSKPESSSVLTALDKIEGVFERPSDEVIRELSRALRQALGVSLFGIDIIINNQTGQHAVIDINAFPGYEGVSEFFTDLLNHVATVLQGQSYMEDDRICSPPFMELTSLCGDDTMRLLEKNGLTFPFICKTRVAHGTNSHEMAIVFNQEGLNAIQPPCVVQNFINHNAVLYKVFVVGESYTVVQRPSLKNFSAGTSDRESIFFNSHNVSKPESSSVLTELDKIEGVFERPSDEVIRELSRALRQALGVSLFGIDIIINNQTGQHAVIDINAFPGYEGVSEFFTDLLNHISPKTCSPVLEVITQLRFPVSDSERFGVPEQVVVMDSSVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNHGGVIFKVYVVGDHVKCVKRRSLPDISEEKIGTSKGSLPFSQISNLTAQEDKNIEYGEDRSLEKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKMPSYEPVLTEFFWDMVTKKN"
  },
  {
    "reference": "MATATQIFLSPDHAGIARVSECPVGAIEAANHLLQTNHDEHHMFWRPVAGHNHLTHSVLTILALGGGPAELQRAFDDARDIQRPIPPVDRQVVEQLGDPEQFRSRIGQLDQYSNFLAFFSQEIATKGYRAVVDEHCFSGSRNAETLFAQLYEGLYHPVIHLAFGIEFEQPSIVAEALAQVASHDSMGIEAFLMDCEAEATQSAHSGRTLVQLFRDAEADEALRHAAEGFDDGPARVRDGVLGRTARAITALAAQFRVDPQDIEHRLAEMINCSAFITGAVQRTGKPRKIDFFHLHTVTASLSIDILVRQPWISPVVKARLVEWKARVDLVWYTATGAVQLHLPSLLNYMPTSSAGMDWAALYRAVAAVHDDGHLAKLVRALKSGEAVSNPFEKGAGETFPIQGIAWLKLAQMAYDTTVDRPIEQKWIWGIGFDENWTHVLSLESEK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that The atrochrysone carboxyl ACP thioesterase gedB then breaks the thioester bond and releases the atrochrysone carboxylic acid from gedC; The atrochrysone carboxylic acid is then converted to atrochrysone which is further transformed into emodinanthrone; Ring cleavage of questin by questin oxidase gedK leads to desmethylsulochrin via several intermediates including questin epoxide; Questin oxidase; part of the gene cluster that mediates the biosynthesis of geodin, an intermediate in the biosynthesis of other natural products The designed protein sequence is ",
    "response#1": "TSTVQKIVEESFDGRAARVVMQSDLMQSDLLEAAYGHKMNGCGVVTSSIHADVGFTLGQYVYKKLNPNTKVPAMNMASLEVLKGLVANKNTDKPQRFQVTVTTTDINSRILQLEWRNVHAHGPAEEPFASAKIYYCDASEWLLSWRPTLHLVQGRIQALERLAEAGIANRFSGRMAYNLFANSLVDYAGKYRGMQSVVMHELEAFADVTLTVEKAGTWTVPPYFIDSVAHLAGFIMNVSDAIDNQKNFCVTPGWNSMRFAAPLVAGGRYRSYVKMIPTVEDDSVYLGDVYILQNDMIIGMVGGIKFRRYPRLLLNRFFSR",
    "response#2": "IANRFSHKMAYTLFGNNLVEYADKYRGMQAVVMHEFEAFADVTLKHVSGGGSYTVPPYFIDSVAHLAGFIMNVSDASDTANTFCVTPGWDSMRFARPLVPGQRYRSYVKMIPTDDDPSVYLGDVYVFQDKVIMGMP",
    "response#3": "TSTVQKIVEESFDGRAARVVMQSDLMQSDLLEAAYGHKMNGCGVVTSSIHADVGFTLGQYVYKKLNPNTKVPAMNMASLEVLKGLVANKNTDKPQRFQVTVTTTDINSRILQLEWRNVHAHGPAEEPFASAKIYYCDASEWLLSWRPTLHLVQGRIQALERLAEAGIANRFSGRMAYNLFANSLVDYAGKYRGMQSVVMHELEAFADVTLTVEKAGTWTVPPYFIDSVAHLAGFIMNVSDAIDNQKNFCVTPGWNSMRFAAPLVAGGRYRSYVKMIPTVEDDSVYLGDVYILQNDMIIGMVGGIKFRRYPRLLLNRFFSS"
  },
  {
    "reference": "MKLNDIINIIEDIAPVNLKEGFDNVGLMVGDREKNITKILLALDCTEEVIKEAKEMGAELILTHHPLLFRKPSTITTDTLLGRKIISLIKNDINLYSAHTNWDSVKGGLNDTLVEILGFNKGIIMDKSPVDSEAGIGRVVELTKEMTVLEIINLIKSSLGVKNLRYAGDLNEVIKKIAIVNGSGQDFFGDAKKLGADLIITGDTTYHFVSDYKEMGLNILDIGHFNSEWPVLIKVSEKVKERLDSDVEFIVSKEAKDPFEFI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must be able to metal ion binding.2. The a divalent metal cation binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MRIGMGYDVHKLVENRDLILGGVKIPYEKGLLGHSDADVLLHAIMDSLLGAAALGDIGKHFPDTDPKYKGADSIKLLEFVGELLNKNNYKISNIDATIIAQRPKMAPHIPTMRENIAKALNIDLDQINVKATTEEGLGFTGSGEGISSQSICLLI",
    "response#2": "MRIGMGYDVHKLVENRDLILGGVKIPYEKGLLGHSDADVLLHAIMDSLLGAAALGDIGKHFPDTDPKYKGADSIKLLEFVGELLNKNNYKISNIDATIIAQRPKMAPHIPTMRENIAKALNIDLDQINVKATTEEGLGFTGSGEGISSQSICLLI",
    "response#3": "MRIGMGYDVHKLVENRDLILGGVKIPYEKGLLGHSDADVLLHAIMDSLLGAAALGDIGKHFPDTDPKYKGADSIKLLEFVGELLNKNNYKISNIDATIIAQRPKMAPHIPTMRENIAKALNIDLDQINVKATTEEGLGFTGSGEGISSQSICLLK"
  },
  {
    "reference": "MNNDTTHFGYQQIASHEKSQRVAEVFRSVAGKYDLMNDLMSLGLHRLWKKITIAESGVRAGHRVLDIAGGTGDLAYAFSKKVGKEGLVVLADINEKMLQYGRDKFIDKGILTTNIQYAQADAESLPFAENYFDCITIAFGLRNVTDKAVALRSMYRVLKPGGKLLILEFSKPVIPMLNKLYDAYSFNVIPKLGAWITHDQDSYQYLVESIRMHPNQITLKNMLEDAGFEDVSYRNLSGGIVALHKGFKY",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity, S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity.2. We require a protein that can specifically bind to the metabolites produced in the menaquinone biosynthesis pathway. The designed protein sequence is ",
    "response#1": "GWSS",
    "response#2": "SPHDKTXP",
    "response#3": "MSIIKKS"
  },
  {
    "reference": "MRSFYSACAAVSAFQTGSSMTNYIFITGGVVSSLGKGIAAASLAAILEARGLTVTLIKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFVRTTMTKRNNFTSGKIYETVIKKERRGDYLGGTVQVIPHITNEIKRSIKQGADNFDVAMIEIGGTVGDIESLPFLEAIRQMRIELGSQRSLFIHLTLVPYIATSGETKTKPTQHSVKELRSIGIQPDILICRSEKPLSLSDRAKIALFTNVEKEGVISLEDAKSIYQIPMILHDQGLDEIVVKKLGLTLKPADLHEWQRVVDAQAIQTMTVKVAMVGKYTELNDAYKSINEALLHAGIHTQTKVEIVYIDAELIEKHGTELLASVDAILIPGGFGERGIEGKIRTVQYAREEKIPFLGICLGMQTAVIEYARNVVGLKGANSTEFDKTTPYPVIGLITEWMAEDGSINQRDENTDLGGTMRLGAQMCQLDVDSQAYKIYNKPQIVERHRHRYEVNNQFVDALVENGLTVSGRSADNTLVEMIELHDHPWFVACQFHPEFTSTPRDGHPLFQQFILAARSYHGQKEKA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that can modulate the overall flux of metabolites through the CTP from UDP: step 2/2, CTP biosynthesis via de novo pathway, Pyrimidine metabolism pathway, enhancing or suppressing its activity as needed.2. The designed protein must possess ATP binding, CTP synthase activity, glutaminase activity, metal ion binding to support 'de novo' CTP biosynthetic process, glutamine metabolic process under certain conditions.3. The designed protein must have at least one CTP synthase N-terminal, Glutamine amidotransferase domain.4. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates..5. The designed protein is the optimization of the Nucleophile, Nucleophile; for glutamine hydrolysis, active site to achieve high catalytic efficiency for H2O + L-glutamine = L-glutamate + NH4(+).6. The designed protein should have a high specificity for UTP, L-glutamine, CTP, Mg(2+), ATP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "KYIFVTGGVVSSLGKGIAAASIGCLLESRGLRVTLQKFDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTYAPLTQSNNVTSGRIYETIISRERRGDYLGKTVQVIPHVTDEIKANIEKVSGGVDVVLVEIGGTVGDIESLPFLEAIRQLRHKKGRENCFFVHVTLVPWIAAAGELKTKPTQHSVKELRTIGIQPDMLICRCEKPLAGDIKDKIALFCDVSADAVIEACDAKSVYEVPLGFASQNVDDIILRRLGLPFRDVDWSVWDDLLRRVHEPEHEVTIALVGKYIELPDAYLSVTEALRAGGFAHQAKVNIRWVASDLCESDEGATSQLADVDGICIPGGFGVRGIEGKLGALKFARENKIPTLGLCLGLQCMVIEFARNVLGLQDASSTEFDPQAKHPVIATMAEQIDILAAGEMGGTMRLGSFEAKLTKGSIAAEVYGSSEVTERHRHRYEVNNSYREQLTAGGMVISGTSPDGSLVEFVELSKADHPYFVATQAHPEFKSRPNRAHPLFIGLVQAALRHKQERALLPDAYLSVTEALRAGGFAHQAKVNIRWVASDLCESDEGATSQLADVDGICIPGGFGVRGIEGKLGALKFARENKIPTLGLCLGLQCMVIEFARNVLGLQDASSTEFDPQAKHPVIATMAEQIDILAAGEMGGTMRLGSFEAKLTKGSIAAEVYGSSEVTERHRHRYEVNNSYREQLTAGGMVISGTSPDGSLVEFVELSKADHPYFVATQAHPEFKSRPNRAHPLFIGLVVKAALKHKQDRALADV",
    "response#2": "IFVTGGVVSSLGKGIAASSLAAILEARGLNVTMMKLDPYINVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFVRTRLTGRNSITTGKIYESVIRKERRGDYLGATVQVIPHITDQIKHSVYEATRGHDVALVEIGGTVGDIESLPFLEAIRQLRIEHGPEKCLFMHLTLVPFIKAAGEIKTKPTQHSVKELRSIGIQPDILVCRCEQPLPDGERRKIALFTNVPEKAVISAVDLDVIYKLPSWLHKQGLDDIVVERLGLEVKPVDLSDWQAMVERVKHPTCELPDAYLSVTEALRAGGFAHQAKVNIRWVASDLCESDEGATSQLADVDGICIPGGFGVRGIEGKLGALKFARENKIPTLGLCLGLQCMVIEFARNVLGLQDASSTEFDPQAKHPVIATMAEQIDILAAGEMGGTMRLGSFEAKLTKGSIAAEVYGSSEVTERHRHRYEVNNSYREQLTAGGMVISGTSPDGSLVEFVELSKADHPYFVATQAHPEFKSRPNRAHPLFIGLVVGAALDARDAKVTVAIVGKYVEHKDAYKSLGEALRHGGIKQNTAVHLKWLDSEQIEAEGAAAVLGDVDAVLVPGGFGKRGFEGKVQAAQYAREHKVPYFGICYGMHAAVVDFARHVAGIEGADSSENDRHAKDPVIALVTEWTTASGEVETRSEQSDLGGTMRLGAQEARLKPGTLAHNLYGRDVVRERHRHRYEFNNRYRQQFEDLGMVVSGKSMDDLLVELVELPPQTHPWFLACQAHPEFTSTPRDGHPLFIGFRGHAR",
    "response#3": "RHIFVSGGVASSLGKGLTAASLGNLLRARGLRVVMQKLDPYLNVDPGTMNPFQHGEVFVTEDGTETDLDIGHYERFLDINLSKAANVTTGQIYSTVIAKERRGEYLGDTVQVIPHITDEIKRRMRLQANEEPIPDVIITEIGGTVGDIESQPFLEAARQIRHDVGRDNVFFVHVSLVPYLGPSGELKTKPTQHSVAMLRSIGIQPDAIVCRSDRALPDSIKKKIALMCDVDIRAVVNAADAPSIYAIPTVLDNEGLDSYIINSLSVTIALVGKYIELPDAYLSVTEALRAGGFAHQAKVNIRWVASDLCESDEGATSQLADVDGICIPGGFGVRGIEGKLGALKFARENKIPTLGLCLGLQCMVIEFARNVLGLQDASSTEFDPQAKHPVIATMAEQIDILAAGEMGGTMRLGSFEAKLTKGSIAAEVYGSSEVTERHRHRYEVNNSYREQLTAGGMVISGTSPDGSLVEFVELSKADHPYFVATQAHPEFKSRPNRAHPLFIGLIGAAIEHARVNIALVGKYAGLSDSYVSMKEALTHAGAAIDTQVCIEYLESEKFEKNPSCIGDLLKYDGIFIPYGFGSRGSEGKIDAINFSRKNDIPFLGICFGFQLAVVEFARNVCGLKNANSIEIAEDSPHPVIDIMPEQRNIKSKGATMRLGSHKIVLKPETLAYSLYKTEQIFERHRHRFEVNLNYLNILKEKGLIFSGNSADGRRMEILELPDQYFFLASQFHGEFKSRPGKPSPTYYGFL"
  },
  {
    "reference": "MAAIAASEVLVDSAEEGSLAAAAELAAQKREQRLRKFRELHLMRNEARKLNHQEVVEEDKRLKLPANWEAKKARLEWELKEEEKKKECAARGEDYEKVKLLEISAEDAERWERKKKRKNPDLGFSDYAAAQLRQYHRLTKQIKPDMETYERLREKHGEEFFPTSNSLLHGTHVPSTEEIDRMVIDLEKQIEKRDKYSRRRPYNDDADIDYINERNAKFNKKAERFYGKYTAEIKQNLERGTAV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Involved in pre-mRNA splicing as component of the spliceosome.2. Incorporate a signal peptide in the protein design.3. The designed protein should be involved in mitotic G2 DNA damage checkpoint signaling, gastrulation, mRNA splicing, via spliceosome by localizing to post-mRNA release spliceosomal complex, catalytic step 2 spliceosome, nuclear speck, nucleoplasm, nucleus and exhibiting RNA binding.4. The target protein must exhibit helix-helix-helix-strand-helix-strand-helix-turn-helix as its primary conformation. The designed protein sequence is ",
    "response#1": "EVQQHTIPQSIHGTDVLCQAKSGLGKTAVFVLSTLQQLDPVPGEVAVVVICNARELAYQIRNEYLRFSKYMPDVKTAVFYGGTPISKDAELLKNKDTAPHIVVATPGRLKALVREKYIDLSHVKNFVIDECDKVLEELDMRRDVQEIFRATPRDKQVMMFSATLSQEA",
    "response#2": "PFKATGKPHTLWVAFAKFYEDNGQLDDARVILEKATKVNFKQVDDLASVWCQCGELELRHENYDEALRLLRKATALPARRAEYFDGSEPVQNRVYKSLKVWSMLADLEESLGTFQSTKAVYDRILDLRIATPQIVINYAMFLEEHKYFEESFKAYERGISLFKWPNVSDIWSTYLTKFIARYGGRKLERARDLFEQALDGCPPKYAKTLYLLYAQLEEEWGLARHAMAVYERATRAVEPAQQYDMFNIYIKRAAEIYGVTHTRGIYQKAIEVLSDEHAREMCLRFADMECKLGEIDRARAIYSFCSQICDPRTTGAFWQTWKDFEVRHGNEDTIKEMLRIRRSVQATYNTQVNFMASQMLKVSG",
    "response#3": "GLQQYYIKLEEREKNRKLAQLLDDLEFNQVIIFVKSTTRANELTKLLNASNFPAITVHGHMKQEERIARYKAFKDFEKRICVSTDVFGRGIDIERINLAINYDLTNEADQYLHRVGRAGRFGTKGLAISFVSSKEDEEVLAKIQERFDVKIAEFPEEGIDPS"
  },
  {
    "reference": "MADSFDVIVVGGGHAGCEAAAAAARTGARTALVSMRRDMIGAMSCNPAIGGLGKGHLVREVDACDGLIARAADRAAIHYRMLNSSKGAAVQGPRVQADRKLYKAAIHDLLAELPSLEIVEGSAERLLLDDVSRGTSGLVLADGRELKAGAVVLATGTFLNARLHFGMDCRTGGRVGEAAAVGLAEQLRALDLPIARLKTGTPPRLDGRTIDWARLDRQPSDGEGWTFSALTPHRLVPQLACAITRTNQRTHDVIRAGLDRSPLFSGAITGVGPRYCPSIEDKVHRFGDRDGHQIFLEPEGLDDPTVYPNGVSTSLPEDVQLAMLRTVPGLERVEILQPGYAVEYDYIDPRALTAGLALRALPGLFCAGQINGTTGYEEAAAQGLAAGMNAAAFAAGRPEIHFDRATSYIGVMIDDLTLQGVSEPYRMLTARAEYRLRLRADNAGTRLTSWADAHGAISAARRMFHVEREARRIAMADCLDRRIAGSAELIAAGAAVAADHARRSLTEWLRFPEVGDAELTVLAPELVDVPEDVRAEVIQDGRYAPYLARQDQEIARLRADERIGLAVIVDYGAIAGLSNEMVERLNAARPADLAAAGRVRGVTPAALAAIMVHARRLAA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must be able to flavin adenine dinucleotide binding. The designed protein sequence is ",
    "response#1": "TVKDVMTKIDDVFMLPETTILNAKTVTEIVNMGYTRIPIYAKTRNNVVSLLFIKDLALMDPDDNFTVKTVCAYNEHVLRFVSEDTPLKTMLEEFKKGDYHLAMVYKKKKSKGKDNFTDLCGLVTLEDIVL",
    "response#2": "TVKDVMTKIDDVFMLPETTILNAKTVTEIVNMGYTRIPIYAKTRNNVVSLLFIKDLALMDPDDNFTVKTVCAYNEHVLRFVSEDTPLKTMLEEFKKGDYHLAMVYKKKKSKGKDNFTDLCGLVTLEDIVI",
    "response#3": "TVKDVMTKIDDVFMLPETTILNAKTVTEIVNMGYTRIPIYAKTRNNVVSLLFIKDLALMDPDDNFTVKTVCAYNEHVLRFVSEDTPLKTMLEEFKKGDYHLAMVYKKKKSKGKDNFTDLCGLVTLEDIVV"
  },
  {
    "reference": "MATSSFNINELVASHGDKGLLATALVDKTAHEQLEEQLQHQRRGLKVYIRNVLDVKDSEVIRTRYGGKYDLHLAQQELAPHGLAGALRLCETLDCLDFFPRSGLRQDLVLDFGGSWVTHYLRGHNVHCCSPCLGIRDKMRHTERLMSMRKVILNDPQQFDGRQPDFCTKSAAECKVQAHFAISIHGGYDMGFRGLCEAMNAHGTTILKGTMMFDGAMMFDDQGFIPELKCQWRKIKSAFSEEEDATCSAAKLNSSVFSRVRNGKTLIAFDFVEESTMSYVHDWDNIKSFMTDQTYSFNGMTYGIERCVIYAGVMTYKIVGVPGMCPPELIRHCIWFPSMKDYVGLKIPASDDLVKWKTVRILLSTLRETEEIAMRCYNDKKNWMDLFKIILGVLSSKSSTIVINGMSMQSGERIDLNDYHYIGFAILLHTKLKYEQLGKMYDMWNASFIWKWFASMSRPFRVFFSTVVKTLFPTLRPREEKEFLVKLSTFVTFNEECSFDGGKEWDVISSAAFVATQAVADGTILAEEKAKKLADRLAVPVEEVTAIPEVSPTPVDQGTACGLETETSELDSLSAQTRSPIARIAERATAMLEYSAYEKQLHDTTVSNLQRIWCMAGGDNKRNSLESNLKFVFDTYFSVDALVNVHFPTGRWMHPVPEGVVYSVGYNEKGLGPKLDSELYIVNGDCVISNSHDLFSITKSLLAPTGTISQVDGVAGCGKTTAIKSMFNPSTDIIVTANKKSAQDVRYALFKSTDSKEACAFVRTADSI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein contains novel (+)RNA virus helicase C-terminal domains that confer a unique function or activity.2. The ideal protein would be one that plays a critical role in RNA processing through hydrolase activity, acting on acid anhydrides within host cell endoplasmic reticulum membrane. The designed protein sequence is ",
    "response#1": "GGHVVSNDCNRSQILWAASDWAIHEVGCVPCVDSTCWVPLTSSISVRNESVIVSGLGSHIDVLAAMASVCSTLGIGEACGTATLTYITFLSRFFMGLNLTGDCECFLYPGAISTFEFTMRALQSMMPNLSGFVSMLSGVPNTLFTIFTNGHWGVILALCLYGTTNNYFKLCLLLLAYSV",
    "response#2": "QVRNSTGLYHVTNDCPNSSIVYEADDTILHTPGCVPCVREGNASRCWVAMSPTVATRDGKLPTPQLRRHIDLFVGGATLCSALYVGDLCGSVFLVGQLFTFSPRRHWTTQDCNCSVYPGHITGHRMAWDMMMNWSPTAALVTAQLLRIPQAIVDMIAGAHWGVLAGIAYFSMVGNWAKVLVVLLLFAGVH",
    "response#3": "GGHVVSNDCNRSQILWAASDWAIHEVGCVPCVDSTCWVPLTSSISVRNESVIVSGLGSHIDVLAAMASVCSTLGIGEACGTATLTYITFLSRFFMGLNLTGDCECFLYPGAISTFEFTMRALQSMMPNLSGFVSMLSGVPNTLFTIFTNGHWGVILALCLYGTTNNYFKLCLLLLAYSS"
  },
  {
    "reference": "MFVVNSAEMQEIDRIATEEWGIPEIVLMENAGLKVVEEIKKRFTNLKDKKITLILGKGNNGGDGLVVARHLCNLGADIKIFLFGQKEFKNSSLVNYNIVKRLPIKVHKLEHENNLHLLRLSLYSTDVIVDGLFGTGFKGEISDFLSKIIKIINDTDCLKVAIDIPSGLNADTGRARECIKANLTVTFALPKIGLVVYPGTSFAGDVKVVDIGIPSQLVRALNIDKRILQLDEIKNNIPERDENCHKGNFGHLLVIGGSLGMMGAVHLACLSAFRMGAGLVSAAIPASIQTNLAVSLPEMITYPLKEMVPGILDSEAEEEILASIEGKSVIVIGPGFGNNEEYVSILKHILEKIDIPIVIDADGINLLARDISILDNIKAPVILTPHPGEMARLLDISTQEVQKNRIELAKKLAENYGIWVVLKGNKTVIATPKGEVLINPTGSPSLATAGSGDVLAGMIGAMLGQQSDETRAICSAVYLHGLAGEHVAETVGKISSKAGDIIKAIPIILEEVLMDK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.2. The protein contains novel YjeF N-terminal domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "LEPAHDMFIFQPHPRGNKYTNGVVAVLAGSEQYPGAGVLTTLAALNTNSGLTYYVGDSPEVISACPEVVMRPRLEDVERFDAAVIGPGRGDCSEELAALLRTKVPLVIDADALTALANNDLLKQAVRRREAITILTPHEGEFKRLSDATGTPLERAAALATDLKCIVLLKGRITTIAEWADSVQAPLLTTVDAGSSWGSVPGSGDVLAGVIAALCANVHARMGFILDLLDVCQENYYSPATAVSLHATAAYLSAQTPYGPAPTRATKIAESIGSAIAML",
    "response#2": "SMRPRRPQSKKGDYGRLLIIGGSETFYGAPTLAAMSAMRASVDLVYVAAPEETAKTIASYSPDLITIKLKGRNLNPDNVKELMPWIERANAVVLGPGMGMSDETVQFSQEIVNLLLGLNKPVVLDADALKANKGRRLSKSFVITPHAGEFAIYFGMQLSMDVKERIGQVIEGSKKCDCVVLAKGYVDVIADGSRFKLNKTGNPGMTVGGTGDTLAGLVGAFLAMGLEPYKAAYLGAFVNGLAGSLAYAELGPHITASDLVYRIPQVMG",
    "response#3": "LLIAGSYGKMGAAVLSARACIRAGVGLLSCHLPKCGYEIMQISEPCAMCIPDKDTYFNTHFHTGLADARYKVIGIGPGIDTQPETARSFEQLLHSWQQPMVIDADALNLLAKNPKLLQLVPKNSILTPHPKEFERLFGPAPNDVERLALLSKEAVSRHLYILLKGRYTAMACPDGSIYFNPTGNPGMATGGSGDVLTGILTGLLAQGYTSKEALILGVWLHGYAGDIAAAEHSQEAMTAGDIAR"
  },
  {
    "reference": "MKRVGLVGWRGMVGSVLMQRMREENDFADIDPVFFSTSQTGQPAPDVGKADVPPLKDAFDLDELRKLDVIVTCQGGDYTNKVFGPLRESGWEGYWIDAASSLRMADHSVIVLDPVNRNVIDEALEKGVKDYIGGNCTVSLMMLALGGLLEKDLIEWVSPMTYQAASGAGAQNMRELIEQMGSLRDGVADQLADPSSAILDIDRQVSETMRGADFPIDNFGAPLAGSLIPYIDKQLESGQSKEEWKAQSETNKILGRSESPIPIDGICVRIGAMRSHSQAMTIKLRKDLPVSEIEALLAGGNDWVKVIPNDRDASIAELTPAKVTGTLNVPIGRIRKLAMGPEYISAFTVGDQLLWGAAEPLRRMLRILQER",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with aspartate-semialdehyde dehydrogenase activity, NADP binding is needed to enhance 'de novo' L-methionine biosynthetic process, lysine biosynthetic process via diaminopimelate, isoleucine biosynthetic process in a specific context.2. A protein that is capable of catalyzing reactions within the L-threonine biosynthesis pathway with high efficiency and specificity.3. The protein should have a high affinity for substrate, phosphate.4. The Acyl-thioester intermediate, Proton acceptor of the protein must be engineered to enable effective catalysis of L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.5. The designed protein should contain one or more Semialdehyde dehydrogenase NAD-binding domains that are essential for its function.6. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.. The designed protein sequence is ",
    "response#1": "MDIAVVGATGMVGESMLEILSARKFPVGVVHALASERSAGTDVNFGNRSLNVENLADFDFSQVQIGFFSAGASVSDEFVPRAAECGCVVIDNTSRFRYEDNIPLVVPEVNPQRIADLAPIKAKYGLTRVIVSTYQAASGAGQSAWEELVAETTAHNAGQEMHANILPVKNAEHHYPLAYNLLPQIDVFEDDGYTHEEWKMIHETKKIMFGDKNSKAVKVTGTAVRVPVPIGHGEAVYFETEAGTGATSGDIQSILSKAPGVVLEDNPKEQLYPQPINAEGKRETFVGRVRPDLENDDSFHMWVVSDNLLPKGIIANPNCTTMAAMPVLKPLHDRARIKRVVVSTYQSVSGAGKDGMDELWDQTKGMYVPGQEKEPSKFTKQIAFNVIPHIDTFLEDGSTKEEWKMVAETKKIIDTSILVTATCVRVPVFVGHSEAINIEFEDFLDEDEARDILREAPGVMVVDKREDGGYITPVECVGDFATFISRIRQDSTIDNGLNLWCVSDNLRKGAALNTVQIAELIAAG",
    "response#2": "MANDIAVVGATGMVGESMLEILSARKFPVGVVHALASERSAGTDVNFGNRSLNVENLADFDFSQVQIGFFSAGASVSDEFVPRAAECGCVVIDNTSRFRYEDNIPLVVPEVNPQRIADADLKGGYNIIANPNCSTIQMLVALKPLHDRARIKRVVVSTYQSVSGAGKDGMDELWDQTKGMYVPGQEKEPSKFTKQIAFNVIPHIDTFLEDGSTKEEWKMVAETKKIIDTSILVTATCVRVPVFVGHSEAINIEFEDFLDEDEARDILREAPGVMVVDKREDGGYITPVECVGDFATFISRIRQDSTIDNGLNLWCVSDNLRKGAATNSIQIAELLVKQYLS",
    "response#3": "MKLGVVGATGQVGQVVLTLVQERHFPAESIRLFASSRTAGQSVSVAGQDXVVEDLETADPTGLDIAIFSAGATVSRTHAPRFAEAGVTVIDNSSAWRLDPEVPLVVSXVNPXALDXAALKAGRKNWIGGNCTVSCMLMALAPIHALSPIERVHVATYQAASGAGHDAMLELEGQVRDQAMGKPLQTKAFGNQLLYNLIPQIDIFTDNDYTKEEMKMYNETCKILHNDEIRVSATCVRVPVLRAHSEAIWVKCAEPLTVEAVREAMSKQKGLLLMDDPAKRSYPMPLHCSMQEPVYVGRLRADLAEPGCVTFWCVADQIMWGAAEPLRRMLRILLEA"
  },
  {
    "reference": "MKVREITEELEYKLLSPYAAKSRESRGRAREEEKCDIRTDFQRDRDRIIHSKAFRRLSHKTQVFISPEGDHYRTRLTHTLEVAQIARTIARALRLNEDLTEAIALGHDLGHTPFGHSGEEVLNKLLKGGFRHSEQSIRVVEVLENDGKGLNLTWEVKDGILNHSTGGKPSTLEGQVVQISDKIAYINHDIDDAIRGRVLKPEDLPKDLIAILGDKHGKRIDTMVRDVIYNSMGKPEVSMSKEIYEATYQLRAFLFEKVYIGSKAKRDEEKAKRVVEQLFYYFYDNVDKMPKEFVRLAEIYGRERAVADYIAGMTDKYALLKYKEIFLPSPWFEQNIFDL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit triphosphoric monoester hydrolase activity. The designed protein sequence is ",
    "response#1": "VMQKGLKDNFADVQVSVVDCPDLTKEPFTFPVKGICGKTRIAEVGGVPYLLPLVNQKKVYDLNKIAKEIKLPGAFILGAGAGPFQTLGFNSEFMPVIQTESEHKPPVNGSYFAHVNPADGGCLLEKYSEKCHDFQCALLANLFASEGQPGKVIEVKAKRRTGPLNFVTCMRETLEKHYGNKPIGMGGTFIIQKGKVKSHIMPAEFSSCPLNSDEEVNKWLHFYEMKAPLVCLPVFVSRDPGFDLRLEHTHFFSRHGEGGHYHYDTTPDIVEYLGYFLPAEFLYRC",
    "response#2": "HVPELVRAEVAVLKILALQFIMSDPRHLETQARQRERIHRVAQLLYAGAPRTLDPIFAAAFNAAGDDGARMRVVVDQIASYTEGRC",
    "response#3": "VGFSDEMTKNERLFKKFMYQKLYHHPRLLPIAERASYVISRLVALYKDNPRLLPEDWQENWPDNEVDRQRHIVDYVAGMTDHYALKRYLP"
  },
  {
    "reference": "MNEQQRLASQQANSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFRNFGTGRKFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELGHLKSLKRRNPDFLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILKDAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTTSHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTDMLKIMARKYSREHYLELVRKIKEAIPDAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVKKERLQRLNTLVNTLAIEKNSRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVASKSLIGQLVKVKITEAKTWSLNGELVEEPIEVK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein can be expressed in cytosol and perform N6-isopentenyladenosine methylthiotransferase activity without triggering undesirable cellular responses.2. The protein should have a high affinity for [4Fe-4S] cluster.3. The designed protein should contain one or more Radical SAM core, MTTase N-terminal domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MKDHPTFCISSQVGCAMACGFCATARGGLKRQLTAGEIVEQVLRLNRDLRAEPYPHLGGRLFNVVFMGMGEPLDNWTNVHQALKILMDDAGLAMSRRRIQISTSGPAEGLRALLQDHPGVGLTLSLGGSNDTDRRRIMPVPGRTPVTGAVDLAAQYARRNGRRATLAWVLIADKTDRPEQARELVALARRGPFKVNLIPLNPLDDGRLEPASDQGVLAFQKILTDAGVPAFIRASGGQ",
    "response#2": "MPPRHLADLDLAGRRAAVAELGEPAFRAQQLSTHYFGRLVRDPAAMTDLPVASRDKIVGDLLPTLLTPQRELTCDDGATRKTVWKLHDNAMVESVLMGYPDRITACVSSQAGCGMACPFCATGQAGLTRNLSIAEIVDQVVYLAGVAASGAVTGSPPRLSRVVFMGMGEPLANYPRVIEAVRRLVAPAPEGLGLSQRHITVSTVGLVPAMRRLIAEELSVTLALSLHAPDDDLRDELVPVNQRWKVAEVLDAAFDYAAQTGRRISIEYALIRDVNDQPWRADLLGRLLHGKLAHVNLIPLNPTPGSKWDASPKPVEREFLRRLRDSGVAATVRDTRGREIDGACGQLAASL",
    "response#3": "MKDHPTFCISSQVGCAMACGFCATARGGLKRQLTAGEIVEQVLRLNRDLRAEPYPHLGGRLFNVVFMGMGEPLDNWTNVHQALKILMDDAGLAMSRRRIQISTSGPAEGLRALLQDHPGVGLTLSLGGSNDTDRRRIMPVPGRTPVTGAVDLAAQYARRNGRRATLAWVLIADKTDRPEQARELVALARRGPFKVNLIPLNPLDDGRLEPASDQGVLAFQKILTDAGVPAFIRASGGQ"
  },
  {
    "reference": "MTTTLTPPSSRREDGDGSVQVHQDASLKIEEGALVIAVYGKGGIGKSTTSSNLSAAFSKLGKRVLQIGCDPKHDSTFTLTHKMVPTVIDILEEVDFHSEELQPEDFVFEGFNGVMCVESGGPPAGTGCGGYVTGQTVKLLKEHHLLEDTDVVIFDVLGDVVCGGFAAPLQHANYCLIVTANDFDSIFAMNRIVQAIQAKAKNYKVRLGGVVANRSAETDQIDKFNERTGLRTMAHFRDVDAIRRSRLKKCTIFEMDKDEEGVEAVQNEYLLLAQNMLDHVEPLEAESLKDREIFDLLGFD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: The L component serves as a unique electron donor to the NB-component of the complex, and binds Mg-ATP..2. We require a protein that can specifically bind to the metabolites produced in the Porphyrin-containing compound metabolism pathway.3. The protein must exhibit oxidoreductase activity, acting on iron-sulfur proteins as donors, ATP binding, 4 iron, 4 sulfur cluster binding.4. The [4Fe-4S] cluster binding site should be stable and able to withstand changes in environmental conditions.5. The protein design should incorporate a binding pocket that specifically recognizes [4Fe-4S] cluster to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "PHVGAMRIAASMQGVHYVLHAPQGDTYADLLFTMIERRDQRPPVTYTTFQARDLGGDTAELVKRSIRDAADRFQPQALLVGESCTAELIQDQPGALAQGMNLPMPVVSLELPAYSKKENWGAAETFYQLIRTLLKPQLPPAGLSKPDPKRWQEQGRRPRVNLLGPSLLGFRCRDDVREVRVLLDELGIDTHVVAPLGASPEDLRRIPQAEANICLYPEVADSSCRWLERQFGMPTVSTVPIGIGATEMFCRELQELLGLEPTSQGSGQSRMPWYSRSVDSTYLTGKRVFIFADATHAIAAARIASRELGFEVVGLGSYSREQARAVRAAAEELGLAALISDNYLEVEAAMAEAAPELVLGTQMERHSAKRLGIPCAVISSPLHVQDVPARYAPQMGWEGANVIFDSWVHPNDPTAVALKYNADATKSDRLAAARPGLPPAEQHCANCQFHLDDVAGATEEWHGCSLFPGKLINVDGWCASWRR",
    "response#2": "PHVGAMRIAASMHGVHYVLHAPQGDTYADLLFTMIERRGQRPPVTYTTFQARDLGGDTAELVKRHVQEAADRFQPDALLVGESCTAELIQDQPGALAQGMGLPMPVVTLELPAYSKKENWGASETFYQLMRGLLKQSVPPQPSHDVQAWKHEGRRPRVNLLGPSLLGFRCRDDVLEVQRLLSLHGIDVGVVAPLGAGVEDILRLPQADLNVCLYPEVAESSCSWLERNFGIPFSKTVPIGMGATHDFLVEVHALLGMTPPEAAEGYQRSRMPWYSESVDSTYLTGKRVFIFGDGTHAIAAARICSEELGFTVVGLGSYSREMARPVRAAAKKLGLEALICDDYLAVEAAMAEAAPELVLGTQMERHSAKRLGIPCAVISTPMHVQDVPARLSPQMGWEGANVIFDAWVHPQRNFDGFWPHVGAMRIAASMQGVHYVLHAPQGDTYADLLFTMIERRDQRPPVTYTTFQARDLGGDTAELVKRSIRDAADRFQPQALLVGESCTAELIQDQPGALAQGMNLPMPVVSLELPAYSKKENWGAAETFYQLIRTLLKPQLPPAGLSKPDPKRWQEQGRRPRVNLLGPSLLGFRCRDDVREVRVLLDELGIDTHVVAPLGASPEDLRRIPQAEANICLYPEVADSSCRWLERQFGMPTVSTVPIGIGATEMFCRELQELLGLEPTSQGSGQSRMPWYSRSVDSTYLTGKRVFIFADATHAIAAARIASRELGFEVVGLGSYSREQARAVRAAAEELGLAALISDNYLEVEAAMAEAAPELVLGTQMERHSAKRLGIPCAVISSPLHVQDVPARYAPQMGWEGANVIFDSWVHP",
    "response#3": "PHVGAMRIAASMQGVHYVLHAPQGDTYADLLFTMIERRDQRPPVTYTTFQARDLGGDTAELVKRSIRDAADRFQPQALLVGESCTAELIQDQPGALAQGMNLPMPVVSLELPAYSKKENWGAAETFYQLIRTLLKPQLPPAGLSKPDPKRWQEQGRRPRVNLLGPSLLGFRCRDDVREVRVLLDELGIDTHVVAPLGASPEDLRRIPQAEANICLYPEVADSSCRWLERQFGMPTVSTVPIGIGATEMFCRELQELLGLEPTSQGSGQSRMPWYSRSVDSTYLTGKRVFIFADATHAIAAARIASRELGFEVVGLGSYSREQARAVRAAAEELGLAALISDNYLEVEAAMAEAAPELVLGTQMERHSAKRLGIPCAVISSPLHVQDVPARYAPQMGWEGANVIFDSWVHPLGGPEYLPYADPHVGAMRIAASMHGVHYVLHAPQGDTYADLLFTMIERRGQRPPVTYTTFQARDLGGDTAELVKRHVQEAADRFQPDALLVGESCTAELIQDQPGALAQGMGLPMPVVTLELPAYSKKENWGASETFYQLMRGLLKQSVPPQPSHDVQAWKHEGRRPRVNLLGPSLLGFRCRDDVLEVQRLLSLHGIDVGVVAPLGAGVEDILRLPQADLNVCLYPEVAESSCSWLERNFGIPFSKTVPIGMGATHDFLVEVHALLGMTPPEAAEGYQRSRMPWYSESVDSTYLTGKRVFIFGDGTHAIAAARICSEELGFTVVGLGSYSREMARPVRAAAKKLGLEALICDDYLAVEAAMAEAAPELVLGTQMERHSAKRLGIPCAVISTPMHVQDVPARLSPQMGWEGANVIFDAWVHP"
  },
  {
    "reference": "MFETCEAIVMRTVDYGETNKIVTLFTREWGKVAAMARGAKKPSSRLSAVTQPLSYGHYVIRRSRGVGVLHQGELIDSMRALREDLFAAAYAVYIVELTDKSTEEQKRNPYLFELLLQTLQYMSEGRDLEILTFIYEMKMLAVLGIPPVLDRCARCGATEGRFSFSVKEAGFLCHRCEASDPHRFPLSSASVRLLRLFFHIDLARLGSISVKESTKMELGAVLSAYYDEYAGLSLKTKRFLQQIGELKDKLSPDGGQGASFSL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design must be focused on enhancing the efficiency of DNA recombination.2. The protein must exhibit the following characteristics: Involved in DNA repair and RecF pathway recombination.. The designed protein sequence is ",
    "response#1": "MAERRLTGLALKVGPLGEHDRLLSLLSNAEGVSRFAVPGARRPRSSLAAAAPLTLLELQVGGRSGLARVRQLRVLRSR",
    "response#2": "MKLYKADAIVLRARDCGEGDKILTLYSREHGRIKAMAHGVSKPTSRKRGAVQPFTRSRFLLRRGRELDTVSQCEGVETC",
    "response#3": "MEWTDDGIILGTRRHGEANAIVELLTRSHGRHLGLVRGGAGTRMRPLLQPGNSVGAVWRARLDEHLGYV"
  },
  {
    "reference": "MAKEKQEEQQKQTAPENEKAPKKDIKKEASDKKGDQTSKLKEEIADLKKQLADKDDKYLRAEAEIQNMTNRFNKERAQILKYDGQDLAKSILPVLDNLKRALAIEVVDDNGKQLKKGIQMVHDHLVKALNDHGITEIKADGETFDPTLHQAVQTVPVEEGQKPETVVNVLQAGYQLKDRVLRPAMVVVAQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.; GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle; Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex2. The designed protein should have chaperone binding, adenyl-nucleotide exchange factor activity to facilitate protein folding. The designed protein sequence is ",
    "response#1": "MFGEND",
    "response#2": "MPEDKKQDLK",
    "response#3": "MLIQLNYF"
  },
  {
    "reference": "MQPTLAPAPHPSMQTSAQDHADQVLHDQLLAAHQHLSHPQQPRPQPPAAQPPHMQPNTTSPRDQNNIDPAISGAAMLSGPPQTPPQPEPTGQESPKTYGKRPLSTSKRAAQNRAAQRAFRQRKESYIRKLEEQVKEFDTMSEAFKALQAENYQLREYIINLQSRLLESQGEVPELPGNIDLSQPRTDLNVPQPGAGPATTSSSAPAPPSGAQQAQPPQGAASNDDMNSLNRIAVAGLGMRKHPNEEANYLGNNFTGRRTRPDETQADSEVTKTEQAPHGLPMVS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein can be targeted to nucleus and perform DNA binding, DNA-binding transcription factor activity.2. The designed protein should contain one or more bZIP domains that are essential for its function.3. The designed protein's functionality will benefit from Pro residues, Polar residues compositional bias in its structure.4. The protein must exhibit the following characteristics: Putative transcription factor.. The designed protein sequence is ",
    "response#1": "MGSNDPSTPSKASKPPEQEQPPATTSGTTAPVYPEWPGFQGYPAMPPHGFFPPPVAAGQAHPYMWGPQHMVPPYGTPPPPYMMYPPGTVYAHPTAF",
    "response#2": "MGSNDPSTPSKASKPPEQEQPPATTSGTTAPVYPEWPGFQGYPAMPPHGFFPPPVAAGQAHPYMWGPQHMVPPYGTPPPPYMMYPPGTVYAHPTAY",
    "response#3": "MGSSEAETPAKANKASAPQEQQPPATSSTATPTVYPDWTSFQGYPPIPPHGFFPSPVVSNPQGHPYMWGPQPMMPPYGSPPYVIYPPGGIYAHPSS"
  },
  {
    "reference": "MLLEALSNILEFYDDLLSKCDARVKDYPLVHCPAKMTTLLVSYVVFAIYLGPRLMANRKPFHLNTVMVVYNFGMVAFNAFIVYEFMMSGWGTTFTWRCDLIDPSSSPQALRMVRAGWLFFFSKFIELLDTVFFVLRKKQNQITFLHVFHHSVMPWSWWWGIMLTPAGGMGSFHAMVNATVHVIMYTYYGLAAAGPRFQRFLWWKKYMTAIQLTQFIVVSVHISQYYFMEKCDYQVPLWIHLIWMYGVLFFVLFSNFWLQAYIRGKRLPVTQLKSGRERTPPAPPLANGTHGTHGTHQHNGSADQNNHNQHSHTNGNVQLGKVKEI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. We are looking for a protein with broad substrate specificity for a very-long-chain acyl-CoA + H(+) + malonyl-CoA = a very-long-   chain 3-oxoacyl-CoA + CO2 + CoA.3. The protein's functional design necessitates the inclusion of a Di-lysine motif motif. The designed protein sequence is ",
    "response#1": "PYRWLSYKQVSDRAEYLGSCLLYKGYKPSPDQFVGIFAQNRPEWIIAELACYTYSMVAVPLYDTLGAEAIVYIVNKAEIATVICDTPQKANVLLENVEKGLTLGLKMIILMDPFEDSLKEKGEKLGVDILSLYDAETLGKENFKKPVPPKPEDLSIVCFTSGTTGDPKGAMLTHRNVVANSASFLKCVENVFMPTCEDVTISYLPLAHMFERVVQAVVYTVGAKVGFFQGDVRLLPDDMKTLQPTVFPVVPRLLNRLYDKVHSEAKTPFKKFMLNLAISAKFSEVKQGIVRQDSIWDTLIFKKVQAGVGGKVRLMVTGAAPISSPVLVFLRAALGCPVYEAYGQTECTAGCTFSSPGDWTTGHVGAPLACNEVKLEDVVDMNYFSVDGEGEICIRGTNVFKGYFKDEHKTDATFTEDGWFKTGDIAEWLPTGTLKIIDRKKNLVKTLNGEYIALEKEVERLLLAHPSITDVAVIGVPDMTWGQRVTAVVTLQEGHSLSHRELKEWARGVLAPYAVPSELLLVEEIPRNQMGREVEAVLSRLLGQTDVAVYGVAVPGVEGKAGMAAIADPHSQLDPNSMYQELQKVLASYARPIFLRLLPQVDTTGTFKLNKKQLRDEFFKDVSNRI",
    "response#2": "QPYEWISYKQVAEMAECIGSALIQKGFKPCSEQFIGIFSQNRPEWVTIEQGCFTYSMVVVPLYDTLGTDAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYDNDLVERGQKCGVEIIGLKALEDLGRVNRTKPKPPEPEDLAIICFTSGTTGNPKGAMVTHQNIMNDCSGFIKATESAFIASPEDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTSVKRWLLDFASKRKEAELRSGIVRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPCNYIKLVDVEDMNYQAAKGEGEVCVKGANVFKGYWNDPQKTAETYVMTRPRPVYLVDFSCYLPPDHLKAPYARFMEHSRLTGDFDDSALEFQRKILERSGLGEDTYVPEAMHYVPPRISMAAAREEAEQVMFGALDNLFANTNVKPKDIGILVVNCSLFNPTPSLSAMIVNKYKLRGNIRSYNLGGMGCSAGVIAVDLAKDMLLVHRNTYAVVVSTENITQNWYFGNKKSMLIPNCLFRVGGSAVLLSNKSRDKRRSKYRLVHVVRTHRGADDKAFRCVYQEQDDTGRTGVSLSKDLMAIAGETLKTNITTLGPLVLPISEQILFFMTLVVKKK",
    "response#3": "PYRWLSYKQVSDRAEYLGSCLLYKGYKPSPDQFVGIFAQNRPEWIIAELACYTYSMVAVPLYDTLGAEAIVYIVNKAEIATVICDTPQKANVLLENVEKGLTLGLKMIILMDPFEDSLKEKGEKLGVDILSLYDAETLGKENFKKPVPPKPEDLSIVCFTSGTTGDPKGAMLTHRNVVANSASFLKCVENVFMPTCEDVTISYLPLAHMFERVVQAVVYTVGAKVGFFQGDVRLLPDDMKTLQPTVFPVVPRLLNRLYDKVHSEAKTPFKKFMLNLAISAKFSEVKQGIVRQDSIWDTLIFKKVQAGVGGKVRLMVTGAAPISSPVLVFLRAALGCPVYEAYGQTECTAGCTFSSPGDWTTGHVGAPLACNEVKLEDVVDMNYFSVDGEGEICIRGTNVFKGYLNNPEATAATIDEEGWLHTGDIAVFNHDGSLKIIDRKKELIKYKGFQVAPAEVEAVLSRLLGQTDVAVYGVAVPGVEGKAGMAAIADPHSQLDPNSMYQELQKVLASYARPIFLRLLPQVDTTGTFKKTLKKELVDKYKG"
  },
  {
    "reference": "MNIMELLKPLRNKQIESYTDTDITGLSANSDTIRPGDMFVCLPGTKSDGHIFIQDAAQKGCSAILAERIVENRPPGIPYIIVPDTRKAMAILADQFYEHPSYGMKLIGVTGTNGKTTTSHLIERILSDYGHSTGLIGTLTMKIDAYVEKTTHTTPDAIELQAFLRKMKDRGAEYAVLEVSSHALAMGRTRGCDFKTAVFTNLTHDHLDYHRDMGRYLEDKALLFTGLGNSYAGEEEKTAILNADDPASAYLAARTAVQTFTYGIREQADVRARDIEIRPDGTYFRVKTPVGEADIRLRISGEFNVYNALAAIAACLAEDIPLEDIRRSLASFEGLAGRFQRIDDGQPFHVIVDYAHNPDGLEKAIETARRISRGGVITVAGCEGDRDKAKRPLMASIAAGLSDWAILTSDNPRSENPEAILADMTKGIPPQDEPRWEGIVERREAIYRALSYAREGDCVLITGKGHEQRQIFAENRSVPFDDAEVALEWLKAAKFESEPSGKRRKH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein's functional design necessitates the inclusion of a Meso-diaminopimelate recognition motif motif.2. We are looking for a protein with broad substrate specificity for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.3. The designed protein should be optimized for ATP binding activity in order to improve cell division, regulation of cell shape, peptidoglycan biosynthetic process. The designed protein sequence is ",
    "response#1": "MSVTGTNGKTSTVEMTRQLWRMAGERAASIGTLGVTTPDESVSTGLTTPDIVTFLSNLRGLAREGTTHIAYEASSHGLSQYRNEGVPVKAAAFTNFSRDHLDYHATMEDYFEAKMRLVEKVLDDDGTAVIWADDPKADDVIARAKKRGLEVMTVGKTGEDIVLVSQVPTELGQEIVVRHAGIEKKIALPLIGAYQAANALTAAGLAISCGEAPEQVFETLSHLQGAKGRMNRLGGGDLPLVVVDYSHKPDPLEQALESLRGHLRGRLVCVFGCGGERDTGKRPQMAAIAERLADEVVVTDDNPRGEDGDAIVAGIMAGFAHPRRVVVQRDRRAAISGAIGRAKAGDIVLIAGKGHETGQIVGDTVLPFDDVEQASLAVAALEGRA",
    "response#2": "MAELIAGLVLDSRDVREGDAFVAIGGFGTHGLHFVEQARRAGARAILFEPPVPDDVPPPPADAIAVPGLRARMGAMADEFFGEPSKQLWMVGITGTNGKTTTAAAIYSLLLDLGARVGLIGTIANYIGDKILPTQNTTPDSWELQKLFARMREDGCESVVMEVSSQALMLHRVDGCEFDVAVFTNLSQDHLDFHENMQEYFEAKSLLFSRLGGGDRKKFAVINLDDTYGRRLAEVSEAECLTFGIDSGCDLRAENITLSQRGTRFDLVYRGETFPVELPLAGRFNVYNALAAITACLLEGVDLKTIKEGLASFPGVP",
    "response#3": "MIEIAGLVLDSRDVREGDAFVAIGGFGTHGLHFVEQARRAGARAILFEPPVPDDVPPPPADAIAVPGLRARMGAMADEFFDRPAARLNVVGITGTNGKTTTAAAIYSLLLDLGIRTGLLGTVAYRIGDEIIEAQRTTPESTDLQALLARMVAAGCEAAVMELSSHALVLDRVHACRFSVGVFTNLTQDHLDFHGDMETYFRAKKLLFSELNPGAALVNLDDPAGRRLWEESDAPVVSYGVDDGADLRAENVRVGPAGTRFTLVTPGGGEVEVHLRIPGRFSVYNALAALAACVAHGVPLEVAVAGLASFPGVPGRLDLVGATEDGAPAYVDYAHKPDALENVLTSVRPFTTGRVIVVFGCGGDRDTGKRPMMGEIATRLADVVIVTDDNPRSEVPAAIRAEIMAAAPGAIEIGDRRQAIRTAVDMMKAGDTLIVAGKGHETYQIIGDTVIPFDDRKVARAALTDRLEARAAA"
  },
  {
    "reference": "MGSEATTAVNNLQQPLLESTKSEADFRMESVLTDTHLSYFRRIYLASLIEMKYLFHLAAPAIFVYVINNGMSMLTRIFAGRLGSMQLAAASLGNSGFNMFTLGLMLGMGSAVETLCGQAHGAHRYDMLGVYLQRSTIVLVITGLPMTLLFIFSKPLLISLGEPADVASVASVFVYGMIPMIFAYAVNFPIQKFLQSQSIVTPSAYISAATLVIHLILSWLSVFKFGWGLLGLSVVHSLSWWIIVLAQIIYIKISPRCRRTWDGFSWKAFDGLWDFFQLSAASAVMLCLESWYSQILVLLAGLLKDPELALDSLAICMSISAMSFMVSVGFNAAASVRVSNELGAGNPRSAAFSTAVTTGVSFLLSLFEAIVILSWRHVISYIFTDSPAVAEAVAELSPFLAITIVLNGVQPVLSGVAVGCGWQAYVAYVNIGCYYIVGIPIGYVLGFTYDMGARGIWTGMIGGTLMQTIILVIVTFRTDWDKEVEKASRRLDQWEDTSPLLKQ",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must possess xenobiotic transmembrane transporter activity to support xenobiotic detoxification by transmembrane export across the plasma membrane under certain conditions.2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "MKIIAKIPAWMLLCLFILSPTTETIYTSGLPSLTKYFGIDGGITQTTSTLYFLGFALGILTLGRLSDIYGRRPIALLGLFIYVISSIISIFAVNIEMLMIARFVQAFGVSVGSVIGQAMARDSYQGSELSYVYASLSPWLLFIPSLGSSIGGYIIEYSSWHYVFVFFSLTGTILLALYYKVLPETNSYIDFSQSSKYFEVLQVIIKDKILWLYAFIIGAFNGIYYGFFIEAPFIFIDKMKVSSSFYGKLAFLLSFAAIFGGFLGGYLIKKRHVHDKKVMGLGFIFSLCGCILFAVNAFILEVVSASHSLAIAMIFVPMMIHMVGHNLLIPMTLRYALVDYAKVTGTAGSIFGAIYYVVIAAVTYLVSKIHSETISNFALLCFVLSISSAISFYCIWVLYKKKK",
    "response#2": "RIRLTWISFFSYALTGALVIVTGMVMGNIAEYFNLPISSMSNTFTFLNTGILVSIFLNAWLMEIIPLKRQLVFGFILMILAIAGLMVGHNLAIFSACMFVLGVVSGITMSIGTFLITHIYTGRQRGSRLLFTDSFFSMAGMVFPIIAATLLAQHVAWYWVYACIGVLYLAIFILTLCSDFPQLGKQAVGDSQPVRKEKWGIGVLFLSIAALCYILGQLGFIQWVPEYAAKRFGMSIEESGGLVSNFWTAYMVGMWFFSVALRFFDLQRIVTVLAALSTFMMYMFVSSQQSAMLSMYILGLGFVSSAIYTTLITLGSQQTKVSSPKLVNFILTCGTIGTMLTFVVTGPIVAHSGAHAALATANGLYLVVFVMCVLLGFVTALSLLLVAVQTCAGLLPPLLIRHGIDVGIRRHVLSALWWAALAGTATVVIRWVVQWGSAMVAGYTGEQVLFRLRSVVFAHAQRLGLDAFEDDGDAQIVTAVTADVEAIVAFLRTGLVVAVISVVTLVGILVALLAIRARLY",
    "response#3": "MKIIAKIPAWMLLCLFILSPTTETIYTSGLPSLTKYFGIDGGITQTTSTLYFLGFALGILTLGRLSDIYGRRPIALLGLFIYVISSIISIFAVNIEMLMIARFVQAFGVSVGSVIGQAMARDSYQGSELSYVYASLSPWLLFIPSLGSSIGGYIIEYSSWHYVFVFFSLTGTILLALYYKVLPETNSYIDFSQSSKYFEVLQVIIKDKILWLYAFIIGAFNGIYYGFFIEAPFIFIDKMKVSSSFYGKLAFLLSFAAIFGGFLGGYLIKKRHVHDKKVMGLGFIFSLCGCILFAVNAFILEVVSASHSLAIAMIFVPMMIHMVGHNLLIPMTLRYALVDYAKVTGTAGSIFGAIYYVVIAAVTYLVSKIHSETISNFALLCFVLSISSAISFYCIWVLYKKKL"
  },
  {
    "reference": "MNCPAMTASPSSSSSSSYSTFRPPPPLLPQLSNDSQRSVVMHCTRLPFEAFAATSSNRLLGKHSLPLRAALVTSNPLNISSSSVISDAISSSSVITDDAKIGVLLLNLGGPETLDDVQPFLFNLFADPDIIRLPPVFQFLQKPLAQFISVARAPKSKEGYASIGGGSPLRHITDAQAEELRKCLWEKNVPAKVYVGMRYWHPFTEEAIEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLERIFREDEYLVNMQHTVIPSWYQREGYIKAMANLIQSELGKFGSPNQVVIFFSAHGVPLAYVEEAGDPYKAEMEECVDLIMEELDKRKITNAYTLAYQSRVGPVEWLKPYTEEAITELGKKGVENLLAVPISFVSEHIETLEEIDVEYKELALKSGIKNWGRVPALGTEPMFISDLADAVVESLPYVGAMAVSNLEARQSLVPLGSVEELLATYDSQRRELPAPVTMWEWGWTKSAETWNGRAAMLAVLALLVLEVTTGKGFLHQWGILPSL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that contributes to heme biosynthetic process, tetrapyrrole biosynthetic process, porphyrin-containing compound biosynthetic process by functioning in mitochondrion, chloroplast membrane with ferrochelatase activity.2. A protein should be able to manipulate the activity of enzymes in the protoheme biosynthesis, protoheme from protoporphyrin-IX: step 1/1 pathway, either by activating or inhibiting their function.3. For general function, the protein need meet that Produces heme for photosynthetic cytochromes, and for proteins involved in abiotic and biotic stress responses; May play a role in the quality control of individual chloroplasts during photo-oxidative stress through regulation of heme biosynthesis.; Catalyzes the last step of heme biosynthesis by inserting ferrous iron into protoporphyrin IX to produce protoheme4. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "ISGLAVAHHLRSRGTDAVLLESSARLGGAVGTHALAGYLVEQGPNSFLDREPATRALAAALNLEGRIRAADPAAKRRYVYTRGRLRSVPASPPAFLASDILPLGARLRVAGELFSRRAPEGVDESLAAFGRRHLGHRATQVLLDAVQTGIYAGDVEQLSVAATFPMLVKMEREHRSLILGAIRAQKAQRQAALPAGTAPKLSGALSTFDGGLQVLIDALAASLGDAAHVGARVEGLAREDGGWRLIIEEHGRRAELSVAQVVLAAPAHATAKLLRPLDDALAALVAGIAYAPIAVVHLGFDAGTLPAPDGFGFLVPAEEQRRMLGAIHASTTFPFRAEGGRVLYSCMVGGARQPGLVEQDEDALAALAREELKALAGVTARPSFTRVFRWPLGIPQYNLGHLERVAAIDAALQRLPGLHLIGNAYKGVGLNDCIRNAAQLADAL",
    "response#2": "RAMAELIAEQVRLSDVPEEAQVFFSAHGVPKSYVEQAGDPYQKEIEACTTLIMAELENLLGYGNPHSLAYQSRVGPEEWIQPYTDQVIEQLGEAGTRDLVVVPISFVSEHIETLAELDIEYRELATEAGIVHFRRVPAGQDPDRLAALAERVRQHWQNNGRADKLILSYHGVPLRYLHAGDPYHCQCLATSRLLAEALQLTESEVITSFQSRFGREEWLQPYTDVLLKQLPGAGVKSVQIMCPGFSSDCLETIEEIGEENREYFLQNGGENYQYIPALNDDPRFAAALDERAMAELIAEQVRLSDVPEEAQVFFSAHGVPKSYVEQAGDPYQKEIEACTTLIMAELENLLGYGNPHSLAYQSRVGPEEWIQPYTDQVIEQLGEAGTRDLVVVPISFVSEHIETLAELDIEYRELATEAGIVHFRRVPAGDPDRLAAALRRAMAELIAEQVRLSDVPEEAQVFFSAHGVPKSYVEQAGDPYQKEIEACTTLIMAELENLLGYGNPHSLAYQSRVGPEEWIQPYTDQVIEQLGEAGTRDLVVVPISFVSEHIETLAELDIEYRELATEAGIVHFRRVPAGQDPDRLAAALDEVGVLLLNLGGPEKLEDVRPFLFNLFADPEIIRLPFPWLQKPLAWLISTLRAKKSQANYAEIGGGSPLLQITEAQASALTTRLERLGQDAKVYIGMRYWHPFTEEAVEKIKGDRLQRLVILPLYPHFSISTSGSSFRVLEEMWHNDPSLRQLDYSLIPSWYDRAMAELIAEQVRLSDVPEEAQVFFSAHGVPKSYVEQAGDPYQKEIEACTTLIMAELENLLGYGNPHSLAYQSRVGPEEWIQPYTDQVIEQLGEAGTRDLVVVPISFVSEHIETLAELDIEYRELATEAGIVHFRRVPA",
    "response#3": "ISGLAVAHHLRSRGTDAVLLESSARLGGAVGTHALAGYLVEQGPNSFLDREPATRALAAALNLEGRIRAADPAAKRRYVYTRGRLRSVPASPPAFLASDILPLGARLRVAGELFSRRAPEGVDESLAAFGRRHLGHRATQVLLDAVQTGIYAGDVEQLSVAATFPMLVKMEREHRSLILGAIRAQKAQRQAALPAGTAPKLSGALSTFDGGLQVLIDALAASLGDAAHVGARVEGLAREDGGWRLIIEEHGRRAELSVAQVVLAAPAHATAKLLRPLDDALAALVAGIAYAPIAVVHLGFDAGTLPAPDGFGFLVPAEEQRRMLGAIHASTTFPFRAEGGRVLYSCMVGGARQPGLVEQDEDALAALAREELKALAGVTARPSFTRVFRWPLGIPQYNLGHLERVAAIDAALQRLPGLHLIGNAYKGVGLNDCIRNAAQLADAL"
  },
  {
    "reference": "MGSKLSKCSIVGWPAVRERLRRAEPAAEGVGAASRDLERYGALTSSNTAANNADCAWLEAQEEAEVXFPVRPQVPLRPMTYKAAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGVRYPLTFGWLYKLVPVDPREVEEANEGDTSCLLHPMSQHGQEDAHGEVLRWKFDSQLGRRHIARELHPEFYKDC",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have SH3 domain binding, GTP binding within membrane, host cell plasma membrane, extracellular region, host cell Golgi membrane, virion component to regulate suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host autophagy, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I.2. The designed protein should contain at least one Diacidic; necessary for CD4 internalization, Dileucine internalization motif; necessary for CD4 internalization motif to achieve its intended function.3. The protein must exhibit the following characteristics: Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPQVRERIRQTPPAAEGVGAASQDLDKHGALTSSNTPTNNADCAWLQAQEEEEEVGFPVRPQVPLRPMTYKAAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYNTQGFFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPREVEEDNEGENNCLLHPMSQHGMEDEDREVLMWKFDSSLARRHIARELHPEYYKDC",
    "response#2": "MGGKWSKRSGAGWPAVRERMRRAEPAAEPAADGVGAVSRDLERHGAITSSNTAANNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPEEVEKANEGENNCLLHPMSQHGMDDPEGEVLQWKFDSRLAFHHVARELHPEFYQN",
    "response#3": "MGGKWSKSSLVGWPAIRERMRRAEPAEPAADGVGAVSRDLEKHGAITSSNTPTNNADCAWLEAQEDEEVGFPVRPQVPLRPMTYKGAFDLGFFLKEKGGLDGLIYSKKRQEILDLWVYNTQGFFPDWDNYTPGPGVRYPLTFGWCYKLVPVDPAEVEEATEGENNSLLHPICQHGMEDEDREVLRWKFDSALARRHMARELHPEFYKDC"
  },
  {
    "reference": "MHQSSLIESNINEYLKQHEHKSLLRFITCGSVDDGKSTLIGRLLFESKMLFEDQLAAMEADSKKWGTQGEDIDFALLVDGLAAEREQGITIDVAYRFFSTDKRKFIVADTPGHEQYTRNMVTGASTADAAILMVDARKGILTQTRRHSYLMSLLGIRHIVVAINKMDLVGYSEKTFRTIVEEYSRFAEQLGLKHITYIPLSAFKGDNIIVHGDNMPWYHGTTLMGYLETVEVDDTRMQQAPFRLPVQWVNRPNLDFRGFAGTIASGVIKPGDRIRVQPSGKESHVARIVVHGGDLPQAVAGQSVTLTLTDEIDISRGDVISTADAPAHTADQFETTLVWMHEDALLPGRPYLLKIGTKTVTATVTDIKYQVNVNTLEHTAAKKLELNGIGVCNLGLDRAIAFDDYQHNKDTGGFILIDRLTNNTVAAGMLHFALRRSQNIHMQHVDVNKDARALAKGQKPAVLWFTGLSGAGKSTIANLVEKKLHAQGRHTYLLDGDNVRHGLNKDLGFTDADRVENIRRVAEVSRLMVDAGLIVLTAFISPFRSERRLARDLLEDGEFIEVFVDTPLSVAEDRDVKGLYKKARRGELKNFTGIDSAYEQPENPEIRLDTTALSADEAADAVIALLKEKGIINGGDWSI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein with ATP binding functions is required to enable phosphorylation, hydrogen sulfide biosynthetic process.2. The Phosphoserine intermediate active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "MVGHVDHGKSTLVGRLLNDVGALPAGKVDAVAEMCKRRGMKFEWAFVTDALQAEKDQGITIDASHINFSSDRRDYILIDAPGHREFIKNMISGSSDCDGAVVVIDAEEGVCEQSRRHALLLHLLGVTQIVVAVSKMDIVSYDENIFRQIEKDFTECLERFEMEAIRIIPAAGLEGENVKSTTGKLPWYDGPSLFDALEEIEGAIPRFITCGSVDDGKSTLIGRLLHDSHAVLDDQMTALAADSARVGTQGTELDFALLLDGLSAEREQGITIDVAYRFFATERRKFIVADTPGHEQYTRNMVTGASTADAAVILVDARKGVRVQTRRHAYLTSLLGVRHLILAVNKMDLVDWDRATFDAIVAEVRAFAEPLGFAHIHAVPLSALRGDNVVAPSPHAPWSDTPPLLDLLESIPVDRLATAGSVDDGKSTLIGRLLLDTDSLPLDHLDSVTDADGAVDLAALSDGLRAEREQGITIDVAYRFFSTPTRNYILADTPGHERYTRNMFTGASNAHVGVLLVDARAGVLRQTRRHARIASLLGVPHLVAAVNKIDLVDYDESRFRELESELRTLADRLGVADLTVVPVAALDGDNVVQRSDRTPWYDGPTLLGHLEEIDLEKGLLKFITCGSVDDGKSTLIGHMLYDAKLLFTDQKRALELDSKVGSRGGAIDYSLLLDGLMAEREQGITIDVAYRYFTTDHRSFIVADTPGHEEYTRNMAVGASFAELAVILIDATKGVLIQTRRHARICSLMGIKHFVFAVNKMDLIDYDSSRFEKIKREIKMLLAEFDYESVKIIPVSATEGDNITKPSMHMGWYKEETLLSYLENVDIDS",
    "response#2": "MVGHVDHGKSTLVGRLLNDVGALPAGKVDAVAEMCKRRGMKFEWAFVTDALQAEKDQGITIDASHINFSSDRRDYILIDAPGHREFIKNMISGSSDCDGAVVVIDAEEGVCEQSRRHALLLHLLGVTQIVVAVSKMDIVSYDENIFRQIEKDFTECLERFEMEAIRIIPAAGLEGENVKSTTGKLPWYDGPSLFDALEEIEGAIPRFITCGSVDDGKSTLIGRLLYDSRMIFEDQMAALEADSRRVGTQGDQIDFALLVDGLAAEREQGITIDVAYRFFSTDKRKFIVADTPGHEQYTRNMVTGASTAEAAVILIDARKGVLTQTRRHSYLVSLLGIRHVVLAVNKMDLVGWDQAVFDRIVEDYRAFATRIGLADFTPIPISGLGGDNMASRSEASPWYDGPILMDWLESVEVEKRLLRFITCGSVDDGKSTLLGRLLYDAGMVPEDQLAVLTRDSRKLHAELGDKLDFSLLTDGLDAERQQGITIDVAYRYFHTARRSFIVADCPGHEQYTRNMATGASNAELAVVLVDARKGLLTQTRRHTYICGLLGIRQVVLAVNKMDLVGYDQAVYDAIADEYCVLAKDLGIARVQCLPVVAPTGENVGTRSSCMTWYDGPCLLELLESTDATIAD",
    "response#3": "MVGHVDHGKSTLVGRLLNDVGALPAGKVDAVAEMCKRRGMKFEWAFVTDALQAEKDQGITIDASHINFSSDRRDYILIDAPGHREFIKNMISGSSDCDGAVVVIDAEEGVCEQSRRHALLLHLLGVTQIVVAVSKMDIVSYDENIFRQIEKDFTECLERFEMEAIRIIPAAGLEGENVKSTTGKLPWYDGPSLFDALEEIEGAIPRLATAGSVDDGKSTLIGRLLLDTDSLPLDHLDSVTDADGAVDLAALSDGLRAEREQGITIDVAYRFFSTPTRNYILADTPGHERYTRNMFTGASNAHVGVLLVDARAGVLRQTRRHARIASLLGVPHLVAAVNKIDLVDYDESRFRELESELRTLADRLGVADLTVVPVAALDGDNVVQRSDRTPWYDGPTLLGHLEEIDLEIKARLFWLRQEPLHVGESFKLRLATAEHDVTIASIDSVLDLENLTQNPGDEVPAEGFAEVTLAASENILFDPFTPGTADGRGVLVDRQQRIVPWF"
  },
  {
    "reference": "MNEPSRVAAIVVAAGRGSRAGEGVPKQYRALAGQPVIRRSLAALASHPRVGQVVAVIHPDDTAAFSDAAQGLGTLPVPGGATRQESVHRGLEALADFAPDLVLIHDAARPLLSHDVIDRLLDALAENEGAVPALPVVDSLRTGETHADGEVDRSRLKRVQTPQAFRYAAIRSAHADAAPDATDDVAVALAAGLRIAFVEGDEKLAKLTYPADFARAEALLGAGRVTRVGQGFDVHRFGPGDHVWLCGVKVPHDHGLVGHSDADVGLHALTDALLGAAAMGDIGDHFPPSDPKWRGAPSDQFLIHARDLILARSGIIDHVDVTLICETPKVGPHREAMRQRVAGLLRLPVEGVSIKATTTEKLGFTGRREGIAAQATASVRLSTVEIK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should interact with enzymes and substrates within the isopentenyl diphosphate biosynthesis via DXP pathway pathway to enhance or regulate its activity.2. A protein with metal ion binding is needed to enhance isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway in a specific context. The designed protein sequence is ",
    "response#1": "RTGTGFDVHRLADGEELWLCGVKIDHPQGLAGHSDADVAIHALVDALLGAIAAGDIGDHFPPSDPQWKGAASDRFLAHAMALVRASGHALANVDVTIICEAPRIGPHKLTMRERLAAIMDVPLDLVSVKATTTERLGLTGRGEGIAAQAVATVMY",
    "response#2": "MRIGYGYDIHRFKEGRPCVLGGVTIDSPIGPDGHSDADVLIHALMDAMLGAAGMRDIGYYFPPEDDAFKGISSRLLLEKVVQLLKDSNFGIYNADIMVIAEAPKLKPHIEKMKENLSVDLGIPLSRISIKATTNEGLGALGRTEGIAAQAVVSMYR",
    "response#3": "MRIGYGYDIHRFKEGRPCVLGGVTIDSPIGPDGHSDADVLIHALMDAMLGAAGMRDIGYYFPPEDDAFKGISSRLLLEKVVQLLKDSNFGIYNADIMVIAEAPKLKPHIEKMKENLSVDLGIPLSRISIKATTNEGLGALGRTEGIAAQAVVSMYN"
  },
  {
    "reference": "MYKHILVAVDLSEESLILLRKGAGLAEKCGAKLSLIHVDVNFSDLYTGLIDINMSSVQDGVIEETTKALDELALKIDYPVSQRLNGTGDFSQVLEEAVAKYQIDLLITGHHQDFWSKFMSSTRQVMNNVTVDMLVVPLIDE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Required for resistance to DNA-damaging agents..2. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "IGLSLEECDLERTLLGGQSFRWRSICDGNRTKYGGVVFNTYWVLQQEESFITYEAYGTSSPLATKDYSSLISDYLRVDFDLKVNQKDWLSKDDNFVKFLSKPVRLLSQEPFENC",
    "response#2": "FENIFSFLCSQNNNIKRISSMIEWFCATFGTKIGHFNGADAYTFPTINRFHDIPCEDLNAQLRAAKFGYRAKFIAQTLQEIQKKGGQNWFISLKSMPFEKAREELTLLPGIGYKVADCICLMSMGHLESVPVDIHIYRIAQNYYLPHLTGQKNVTKKIYEEVSKHFQKLHGKYAGWAQAILG",
    "response#3": "IGLSLEECDLERTLLGGQSFRWRSICDGNRTKYGGVVFNTYWVLQQEESFITYEAYGTSSPLATKDYSSLISDYLRVDFDLKVNQKDWLSKDDNFVKFLSKPVRLLSQEPFENC"
  },
  {
    "reference": "MFQGGHQPIMVLNQNTKRESGKKAQLANISASKAVSEIVTSTLGPRSMLKMLLDPMGGIVMTNDGNAILREIDVNHPAAKSMIELARVQDEEVGDGTTSVIIMAGEMMSAAKPFIERDIHPSIIVTAYYRALEESIKKIEELAVPIDVNNDDQVNKALSSCIGTKFTSRWGKLITDLALKAVRTIMRGGNLQKLNLEIKRYAKVEKIPGGTLEDSVVLDGVMFNKDITHPKMRRFIKNPRVILLDCPLEYKKGESMTNLEMMKETDMTDALQQEMEELALMCNDILKHKPDVVITEKGVSDLAQHYLLKQNVSVIRRVRKTDNNRISRVSGATIVNRPEEIQESDVGKKCGLFEVKLIGDEYFTFMTECENPEACSIILRGASKDVLNEMERNLHDCLAVAKNIFVNPKLVPGGGAIEMEVSSHLEKISSSIEGLHQLPFRAVAYALEAIPKTLAQNCGVDVVRNITELRAKHNQEGNKFIGIEGNSGKITDMGEANVWEPIAVKLQVYKTAIESACMLLRIDDVVSGLKKQKVAKGGASVTDGNGQEIPETFGDARDG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Molecular chaperone; assists the folding of proteins upon ATP hydrolysis; Known to play a role, in vitro, in the folding of actin and tubulin.2. A protein that localizes to chaperonin-containing T-complex and can perform ATP binding, unfolded protein binding, ATP-dependent protein folding chaperone, ATP hydrolysis activity with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "LTNDAATIIRELEVIHGG",
    "response#2": "MPTITFN",
    "response#3": "LTNDAATIIRELEVIHR"
  },
  {
    "reference": "MAEKIYEPQKIEQEVQALWETNQTFKATEQEDKEKFYCLSMFPYPSGRLHMGHVRNYTIGDVVSRFQRMNGKNVLQPMGWDAFGLPAENAALNNNTAPAKWTYQNIDYMKNQLKSLGFGYDWDREVATCKKDYYRWEQWFFTRLYEKGLVYKKNSTVNWDPVDHTVLANEQVIDGRGWRSGALVEQKEIPQWFIKITDYAEELLTDLEQLEDWPEQVRAMQKNWIGRSEGVDITFNLSENAADISSFDVYTTRPDTLYGVTYVAVAAQHPLALHAAQTRPELAEFIESCKNTKVAEAELATMEKKGCDTGLFAIHPLTGEKVAVWVANFVLMGYGSGAVMSVPGHDQRDWEFAQKYGLPIKQVVEPSADEPQECDLTTAAYTDKGVLVNSAEFNGLAFQEAFDAIAAKLEALGAGSKKVNYRLRDWGVSRQRYWGSPIPMLNLENGESVPVPSDQLPVELPEDVQMNGVISPIKADPDWAKTEYNGQPALRETDTFDTFMESSWYYARYCSAQNQDAMLDPTQANYWLPVDQYVGGIEHAILHLLYSRFFHKLLRDEGLVNSDEPYKRLLCQGMVLADSFFREDESGKKEWFSPADVDTVKDEKGRITQATLKADGKPVEHGGMTKMSKSKNNGIDPQHVIDLYGADTIRVFTMFAAPPEQTLEWVDSGVEGANRFIKRVWKLTQDHIDALDGDTAPAIDKTALTGDQKTLRRAVHRTIAKVTDDVGRRQTFNTAVAAIMELLNSLQRAPQSTDQDKAVLREAIESVV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should contain at least one 'KMSKS' region, 'HIGH' region motif to achieve its intended function.2. The protein can be targeted to cytoplasm and perform leucine-tRNA ligase activity, ATP binding, aminoacyl-tRNA editing activity.3. The protein should have a high affinity for ATP. The designed protein sequence is ",
    "response#1": "RNWIGRIKGFEITLNVFNTCQKLKVFTQRLDLLMGVTYISISSCHKLSINLSKKNELIKKFIKKYRYISQEEQYKVKYTGINTNLFVVHPITKKTIPIWISNATHIEYGTNAVLSIPGHNENDWNFAVKNNLKIKYVIFNPDHQEPKLYTSFLDIKGTLFNSQEFNGLNLKDGTEKIKKIC",
    "response#2": "PHCESDLKLLGVGVDWRRSFITTDVNGYYDSFVRWQFESLKALGKVKYGKRYSIWSTIDDQQCADHERAQGEGVGPQNYTLIKLEVVEPIPECLKEIHSQGKKIYLVPGTLRPETMYGQTNCWILPTGKYGAFEMANGDIFVCTERSARNMSYQQMTTGKGEYKCLAKFEGSDILGAALKAPLAINPIVYVLPMLTIDENKGTGVVTSVPSDSPDDYASLQDLKAKAPLRAKFSIKDEWVLPFEVVPIIDIPGYSNESAVRAYKDLGIKSQNDRALLDQAKDLCYQKGFNDGVMSVGPYAGRKVSEVKKIIKDEMVASGQAVDYSEPTSKVVSRSGDECVVALSDQWYINYGDDDIEWKNQTIKQLESMEFYSAETKKKFEIALGWMNQWACSRSFGLGTHLP",
    "response#3": "PHCESDLKLLGVGVDWRRSFITTDVNGYYDSFVRWQFESLKALGKVKYGKRYSIWSTIDDQQCADHERAQGEGVGPQNYTLIKLEVVEPIPECLKEIHSQGKKIYLVPGTLRPETMYGQTNCWILPTGKYGAFEMANGDIFVCTERSARNMSYQQMTTGKGEYKCLAKFEGSDILGAALKAPLAINPIVYVLPMLTIDENKGTGVVTSVPSDSPDDYASLQDLKAKAPLRAKFSIKDEWVLPFEVVPIIDIPGYSNESAVRAYKDLGIKSQNDRALLDQAKDLCYQKGFNDGVMSVGPYAGRKVSEVKKIIKDEMVASGQAVDYSEPTSKVVSRSGDECVVALSDQWYINYGDDDIEWKNQTIKQLESMEFYSAETKKKFEIALGWMNQWACSRSFGLGTHLP"
  },
  {
    "reference": "MPKSRIRKKADYTPPPSKQATSIKLTSRGWVAPVMLAMFVIGLAWIVVFYVTDGSLPIDSLGNWNIVVGFGFIAAGFGVSTQWK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be able to modulate cell division in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "PMMPKSWFFDTQSVSCDPENSDIVTLQTPLGMAKFIVIENSGCASLCMMVDVEPLALSSTKEIRFCDTIKVMNNRITAYEC",
    "response#2": "PMMPKSWFFDTQSVSCDPENSDIVTLQTPLGMAKFIVIENSGCASLCMMVDVEPLALSSTKEIRFCDTIKVMNNRITAYEC",
    "response#3": "PMMPKSWFFDTQSVSCDPENSDIVTLQTPLGMAKFIVIENSGCASLCMMVDVEPLALSSTKEIRFCDTIKVMNNRITAYEC"
  },
  {
    "reference": "MADLMIKGGTVLTMEGAILNKGVVVVDNGMITFVGKDKDEKADKIIDATGCAVMPGLINAHTHLSMTLFRGYADGLLYGKWIKKIRQAEMKLSPADIRAGAYLGVLEMIKSGTTAFADMYVYMDEVARVVEETGTRAALGYGMIEGINEDPDTKLKSRAGFNKKWNGKAGGRITTMYAPHSAVSCSKEFLIRVKELAGRDNSRIHIHVLETENELRLMKQNYMMCSVNFLDSIGLLSRGVLAAHCIWLSDGDIDILRNNNVNVVHCPSSNMALGTGVAPVPKMLEKGINVALGTDSAASGGSLDMWNEIRTASYLHKLKDPQAMPASTVLGMATVNGAQALGIKAGVLKPGNLADIILVDMKKPQFVSTNLISALIHGASGCDVKTTIVNGEILMEDHEVRVIDEEKVIDGAKAAMLQYS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Can also deaminate adenosine; The preferred substrate for this enzyme is 5'- deoxyadenosine, but all these substrates are efficiently deaminated.2. A protein that can modulate the overall flux of metabolites through the S-adenosyl-L-methionine biosynthesis pathway, enhancing or suppressing its activity as needed.3. The designed protein must be optimized for metal ion binding, 5'-deoxyadenosine deaminase activity, 5'-methylthioadenosine deaminase activity.4. The protein contains novel Amidohydrolase-related domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "TMPGLINTHSHVAMTLLRGVGDDQDLQTWLNEYIWPREAKLNEELVYAGSKLAMAEMIKTGTTMFNDMYFYMEETAKAVEESGMRALLGYGMIDLFDEEKRKSELNETKKFIDKCHNTADGRVQVAVAPHAPYTCSEEILKESKKLADTHNLKLHIHVSETKQEVTDLQKERNETPFEYLNNIGLLDENTIVAHGVWTTPEEMKILKEKDVSISHNPSSNMKLASGIAPVNDYIKNGINVGIGTDGVSSNNNLDMFSEMKLTALLQKVNTLDPKVLPAKETFDMATKNGAKALGVNSGEIKEGKLADIILVNTNVPHMTPVRNPLSNIIYSALGTDVDTVICDGKILYKGDNEKLADAIPGIFNGHTHAAMTLLRGYADDMPLHDWLTTKIWPLEARLTDEAVYWGTKLAALEMIKSGTTFFNDMYWYWESSARAVEESGIRAVLSAVFIDGFDEEKAKEQIIKNKVLYEKSKNLSKRIIFALGPHAVYTVSKESLCWVKEFSERHDLLVHIHVSETKEEVEECKKRYGMRPVEFLAEINLLSSRTIACHCVHLSRKEMELLKQHDVKIVHNPTSNMKLAIGQMPYEELKKTGLYSNIALGTDGCASNNNMDMFEELKIASLVHKAFSGDPTSIPAAEAFALATRNAAKIFRLNSGIIAEGKLADIALLDLRKVELVPNHDLTSNIVYSANGSCVDTVICDGAILYRDNNELEKF",
    "response#2": "TMPGLINTHSHVAMTLLRGVGDDQDLQTWLNEYIWPREAKLNEELVYAGSKLAMAEMIKTGTTMFNDMYFYMEETAKAVEESGMRALLGYGMIDLFDEEKRKSELNETKKFIDKCHNTADGRVQVAVAPHAPYTCSEEILKESKKLADTHNLKLHIHVSETKQEVTDLQKERNETPFEYLNNIGLLDENTIVAHGVWTTPEEMKILKEKDVSISHNPSSNMKLASGIAPVNDYIKNGINVGIGTDGVSSNNNLDMFSEMKLTALLQKVNTLDPKVLPAKETFDMATKNGAKALGVNSGEIKEGKLADIILVNTNVPHMTPVRNPLSNIIYSALGTDVDTVICDGKIVCKNGKIE",
    "response#3": "TMPGLINTHSHVAMTLLRGVGDDQDLQTWLNEYIWPREAKLNEELVYAGSKLAMAEMIKTGTTMFNDMYFYMEETAKAVEESGMRALLGYGMIDLFDEEKRKSELNETKKFIDKCHNTADGRVQVAVAPHAPYTCSEEILKESKKLADTHNLKLHIHVSETKQEVTDLQKERNETPFEYLNNIGLLDENTIVAHGVWTTPEEMKILKEKDVSISHNPSSNMKLASGIAPVNDYIKNGINVGIGTDGVSSNNNLDMFSEMKLTALLQKVNTLDPKVLPAKETFDMATKNGAKALGVNSGEIKEGKLADIILVNTNVPHMTPVRNPLSNIIYSALGTDVDTVICDGKIVAARGL"
  },
  {
    "reference": "MRLLTLALLVAGSLAYKFEDCEQKPAALNQVLRVGAKLYSYNLDFFYQKALPYNAIYAFTDVFLQTNLTNADQYQFYQGTNCTTVQQLYDNDNRYFGILRKAALLRSHQLNPFNDTIVGVSTTEPYEISVLIWKVNYFRVGVYVGAIVLFLLASKLVRNVVFYYTSGCSFGLLASLLLVAFIVWRVAPKKTIGVPILIGGWSVSLYMLHFAWSNLQSIMIEYQKYVIGYFATVLLISMAVCYKRGPPTDARSHDIAQWTLQLVALALIYFSVQMVEVSTGTIGALIIQQICRGFLFAGIRWYFVGLKAVWRKFFPARRRLLNEEEYEEQAEKTTKEQLAQLREYCKKEGNRPWKIAGNVRSARRLARFIEGEDDHITEDEIYAHELTGDVLDDEDYDFNEDYGLRTPNESHFDLEDDEDGAEEWDEVVVRRRASEYGRDSVQSVRVPRSVSSRLLSPYQGQNHMNRSLGPGIGFRRDSTPRHGNFQSEHRPRMPRTEQIYRSRRVEYDVKNGRVEGPSSSTASGMTPSEYMRKARRIDATSKTPTRKSRHPTESEDADE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should exhibit Basic and acidic residues compositional bias.2. The designed protein localizes to the nuclear envelope.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "LMSYLQLFLNAITTVVIFSFILSFIIALQKDLKSTWEQRKHELQYESRICQEQYLTNRCNQTPGLPALGEQCAIWKQCMDRNNDIFFRARSTLSAKLFGDIINSFIDPLNWKTLFVIFCGVITWCFSSNFLLGFVF",
    "response#2": "LMSYLQLFLNAITTVVIFSFILSFIIALQKDLKSTWEQRKHELQYESRICQEQYLTNRCNQTPGLPALGEQCAIWKQCMDRNNDIFFRARSTLSAKLFGDIINSFIDPLNWKTLFVIFCGVITWCFSSNFLLGFVF",
    "response#3": "LMSYLQLFLNAITTVVIFSFILSFIIALQKDLKSTWEQRKHELQYESRICQEQYLTNRCNQTPGLPALGEQCAIWKQCMDRNNDIFFRARSTLSAKLFGDIINSFIDPLNWKTLFVIFCGVITWCFSSNFLLGFVI"
  },
  {
    "reference": "MARDPVTLAQLLTALRGEVEVLEQLGDVSRQIHGITDDSRAVRDKSLFVAVKGERVDGHGFVEQAIKAGAIAVVAQAPVASGPLPFVRVNDSRKALGLLGSRFHGDPSTHLKMIGVTGTNGKTTTTYLCKALLEGIGRQVGLIGTVGYQIGQETFPAPHTTPGALDLQSLLEKMVECGLTAAVMEVSSHALALDRISGCEYDMAIFTNLTQDHLDFHHTMEEYFQAKLQLFTGLAGGKKTGKRAFVNMDDPRGAAIRTACPVTVWGYALKSQADLKAEQVRLSLTGTTFTAATPAGSFPVESRLVGEHNVYNMLGAIGVALHDGATIDQICEATAHITNVPGRFERISSGQDFTVVVDYAHTEDALLRLLTAAQTVKTGRIITVFGCGGDRDRGKRPKMGRVAVEYSDVVVLTSDNPRTEDPMVILHEVEVGVREALVHRNHVQYHLIPDRREAIGAAIREAHQDDMVLIAGKGHEDYQIIGTKKFHFDDREVAREAIQHVRDCA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have a low Km value for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.2. The ideal protein would be one that plays a critical role in cell wall organization, cell cycle, regulation of cell shape, cell division, peptidoglycan biosynthetic process through ATP binding, magnesium ion binding within cytoplasm.3. The protein design should take into account the physiological conditions of the peptidoglycan biosynthesis, Cell wall biogenesis pathway.4. Incorporating a Meso-diaminopimelate recognition motif motif is critical for the successful design of the protein.5. The protein should be able to bind ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate ligand in a variety of conditions6. Mg(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "MKLRDLITDVKNDSRQIKPGDLFIAYPGAAADGRLFIEKAVSLGAAAVAYEPDNFPPSCILPSSVPCVAIPHLAEQLADIAKRFYGYPSQSLQLVGVTGTNGKTSTVEMTRQLWRMAGERAASIGTLGVTTPDESVSTGLTTPDIVTFLSNMSGLAREGVTHVAYEASSHGLSQYRNEGLPVAAAAFTNFSRDHLDYHGTMDEYFEAKMRLFDEVVDAGGTAVVWTGNSEWTAQAIERARQRELRLFTVGEQGEDIRLRARTPTQLGQELEIEHAGETRKLRLPLIGEYQVANALTAAGLAISTGVPLETALRSLEAVPGVPGRMEKYYESNQSIIIVDYAHTPDALEKALCTLRELLRIEGRDARLFCVVGCGGDRDKGKRALVGEVATRLADEVIFTSDNPRSEDPLRIIKDIAAGAVKGNYQLEVDRALAIYQAIANARNGDIVLIAGKGHEDYQILGTTKIHFDDREEAETALQRRFG",
    "response#2": "MIDKINGISENSKEVKKRYIFVARRGTTFNGEIFIEEAIRNGAVVILRESQINSYETYPGVIQIQVKNIKKALSSLALNFYEEPSKKLKLIGVTGTNGKTSVSYFTKTLLNSLGHKTGLIGVTGTNGKTTVATLLYQLFRDMGYKCGLLSTVENQVNGKIIPSTHTTPDPVALNELLDEMVAQGCDYCFMEVSSHAIAQHRIKSLQFSGGIFTNLTHDHLDYHKTFDSYRDAKKAFFDNLPKDAFALTNIDDKNGNVMLQNTRAHKKSYALKSMGDYRAKILENQFGGLLLNIDNEEVWFRMVGTFNAYNLLAVYATAELLGLESLDTLRLLSELESVDGRFESISTDDERLIVVDYAHTPDALDKVLSALIHIKRQDARLWCVFGCGGDRDTKKRPLMAAVAEKWSDEIVVTSDNPRTENPQEILNEIEEGFSESSNVKLMVDRGDAIRYVADHSQPGDVILVAGKGHEDYQEINGERLHFSDRETVKKFLEERL",
    "response#3": "MKLQHLVENLLYKCEINGNTNVAEVKGIAYNSKKVKPGDLFVALKGRNTDGHLFIEEALKNGAVALMVQEERNYPVPYIYVENTRKYLGIISAKFYNDPAKKLTLVGVTGTAGKTSVASFTRQIWAHAGHPAAQIGTTGVVSPTRNDYGSLTTPDPVGLHALLAELAAEGVTHAAMEASSHGLDQCRLDGVRLAAAGFTNLGRDHMDYHPTIESYMAAKMRLFDTLLPKGSPAVIFADDEWSAEAIRVAGKSELQVLTVGRKGDYLALKRVEHFRHKQTAEIHFGDDIFEVDIPLAGDFQVANALVAAGLAISCNIEPDVIKEGLEKAQGVAGRMEKYYESNQSIIIVDYAHTPDALEKALCTLRELLRIEGRDARLFCVVGCGGDRDKGKRALVGEVATRLADEVIFTSDNPRSEDPLRIIKDIAAGAVKGNYQLEVDRALAIYQAIANARNGDIVLIAGKGHETYQEIRGEKYPFDDREVALKAIEDQA"
  },
  {
    "reference": "MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAIKINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must possess metal ion binding, metallopeptidase activity to support proteolysis under certain conditions.2. The designed protein have MPN domains with specific sequence motifs that are crucial for its activity.3. The designed protein should contain at least one JAMM motif motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "MPSLNSPQAVKKYLQLLLGGKPYEAFAVLFLDVKNRLIACEELFRGTLTHTSVYPREIVKEALTHNAASVILAHNHPSGSSEPSAADHSLTQALKQALALIDVRVLDHFVVGGKNVYSFAEHGHR",
    "response#2": "MPRERLIRLGPEKLSNQELLAILLRTGNKEKHVLELSAYLLSSLDSLADLKKFSLQELQRLSGIGKVKAS",
    "response#3": "MPSLNSPQAVKKYLQLLLGGKPYEAFAVLFLDVKNRLIACEELFRGTLTHTSVYPREIVKEALTHNAASVILAHNHPSGSSEPSAADHSLTQALKQALALIDVRVLDHFVVGGKNVYSFAEHGHI"
  },
  {
    "reference": "MLNRVRRTKIVATLGPSTDKANNLEKIILSGANVLRLNFSHGSSEEHKKRAKKAKEIMFKLNCHIALLGDLQGPKIRISKFKKNSIFLKVNDVFILDANLNEKNGNNERVGIDYKKLPNDLNVNDILLLDDGRIQLKVIKIEKCAIFTKVIIGGILSNNKGINKLGGGLSADALTEKDKKDIILASEIDVDYLAVSFPRCADDLIKARELLKKSGSNAQIIAKIERAEAVFDQNAIENIILSSDAIMIARGDLGVEIGDAELVGIQKKLIRTARKLNKVAITATQMMESMILNPLPTRAEVMDVANAVLDGSDAVMLSAETASGKYPSETVISMAKVCKGAEKVPSINVSRHRLNVEFQSIEEAIAMSSMYAANHLKGITAIITMTESGKTALMTSRITSGLPIFALSKHKKTLNLTALYRGVIPIYFDSDKDGVEAANEAIILLCKKKFLFNNDLVIVTQGDIMGKIGKTNTSRILRVTY",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be designed to have high stability and activity towards ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate.2. The protein must exhibit kinase activity, magnesium ion binding.3. A protein that is capable of catalyzing reactions within the glycolysis pathway with high efficiency and specificity.4. The substrate binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "VQKGVNHWDSMNITNASRIYGTEIMIVASASFISVCLVNTGFGPPFISILELRPLRNEIYKSLNPSSYFLYGLRSDMGSTADRSTRYPDDVYDRMWYVANDHAWTSLNTSASISNQSNDGFDVPPKVMSTAATPLDGSNLRILGDARHDDPPSKYHIFLHFTEIQSLK",
    "response#2": "MKKTKIVSTLGPASDDIETITKLAEAGANVFRFNFSHGNHEEHLARMNMVREVEKKTGKLLGIALDTKGAEIRTTDQEGGKFTINTGDEIRVSMDATKAGNKDMIHVTYPGLFDDTHVGGTVLIDDGAVGLTIKAKDEEKRELVCEAQNTGVIGSKKGVNAPGVEIRLPGITEKDTDDIRFGLKHGINFIFASFVRKAQDVLDIRALCEEANASYVKIFPKIESQEGIDNIDEILQVSDGLMVARGDMGVEIPFINVPFVQKTLIKKCNALGKPVITATQMLDSMQENPRPTRAEVTDVANAVLDGTDATMLSGESANGLYPVQSWLPVPMMNLVNGGVHANNRLDMQEFMIIPLGLPNLREAVRCGAEVFSTLRTLLNKRNLPTTVGDEGGFAPSFARNEEALALIVQAIDEAGYQPGSEVAIGVDCASSEFFREGKYHLDVDGLGLTSAQFVDYLATWVEKYPIISIEDGMSEQDWEGWGLLTERLGKTVQLVGDDVFVTNTRILKEGISRNIANSILIKINQIGTLTETLNAIEMAKCAGYTAIVSHRSGETEDTTIADIAVATNALQIKTGSLSRSDRLAKYNQLLRIEEDLGEMAQYAGRSAFR",
    "response#3": "VQKGVNHWDSMNITNASRIYGTEIMIVASASFISVCLVNTGFGPPFISILELRPLRNEIYKSLNPSSYFLYGLRSDMGSTADRSTRYPDDVYDRMWYVANDHAWTSLNTSASISNQSNDGFDVPPKVMSTAATPLDGSNLRILGDARHDDPPSKYHIFLHFTEIQSLK"
  },
  {
    "reference": "MLSLGALVETLRVAGEIRSAPAGLPDIQAIEDDSRRVGPGTLFCAVEGSVQDGHAYVPDAVARGAAAVVVTREVDAAVPQIVVRDGRRALALIASTWFGHPADGLDVIGVTGTNGKTTTVALIRHLLNGEGRAASMGTLGAVDGAGQGLETLGNLTTPGVLGLQAALAELRARGVTTVALEASSHALDQRRLETLTFSAAVYTNLTHEHLDYHLSLESYRDAKSRLSALIGSGGVEIVNADDPAWEGLPSRADVRRVTYGTAVPADVRATDVTLHPQGTTARFVFRDRSLPVRLPLLGDFNVANALAAAATAWALGSEPDGLVARLATAPQVPGRLEKIVAGDWLVLRDYAHTPDALGRAIGALRPITAGRLIVLFGAGGDRDRAKRPLMGRVVAGTADLAIVTSDNPRTEDPDRIIDEIEDGMAGTPHVRITDRRQAIVHALGLLAPGDCLLLAGKGHETYQVIGTASLPFDEPEIVRDALAGGAIG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that can perform UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, tetrahydrofolylpolyglutamate synthase activity, ATP binding in cytoplasm to support regulation of cell shape, cell division, cell cycle, cell wall organization, peptidoglycan biosynthetic process.2. The designed protein have Mur ligase N-terminal catalytic, Mur ligase C-terminal domains with specific sequence motifs that are crucial for its activity.3. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.4. A protein that can modulate the overall flux of metabolites through the peptidoglycan biosynthesis pathway, enhancing or suppressing its activity as needed.5. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.6. The designed protein should have a high specificity for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MKTIHGLQTDSREVSSGDAFIALAGTATSSDHYVDSAIAAGATVVLLESDEEQQCSERHGALIVPIARLRQLTGRIAARFYPSDPAEVLAITGTNGKTTVTQLINQWSTILGTKTATMGTLGNGFYNSLQPTNNTTSSPIFIQLFLSKVLEKQAELVTMEVSSHGLIQHRVKEVPFYIAIFTNLTQDHLDYHENMEKYESAKWLLFSTHKVKKIILNADDQYGKIWLKKLLNFYTVAVTIQNRKQKKYSTKWINATNIEQNNNNSIYITFESSWGTGRISSCLIGHFNVTNLLLSLAAGLALGLPLDVIARGIEKAHRVPGRMERFGGGARPLAVVDYAHTPHALESVLQALREHAAGRLTCVFGCGGDRDAGKRPQMGAIAERLADQVIVTHDNPRREDPAGIVADILAGLREPARASVIGDRRRAIRAALAAAGAGDIVLIAGKGHENYQIVGDTKYPFDDREEAEKALKLRGL",
    "response#2": "MKLTELLNNVQISNLKMDSRTLTPGDLFIAVPGIKKDGRHFIVQAINKKAAAILCETKKKDKHGIFKYIKNVILIYFFKLSENVSLLANRFYDNPSKKLKLVGVTGTNGKTSITFLLGQILEAYHQKTGLIGTIKNIIGDKELESSHTTPESLDLQKLFNKMVDDGCEYCVMEVSSHALELDRVYGCEFEIGVFTNLTQDHLDFHKTFENYYNAKYKLFLQSKCKYQIINIDDEYGKRLIEQTDAEVITYAINKDADICAKDISIDSHGIKFKVITPLGEIEVKLSMIGKFNVYNTLAAISATLHLGFTLEDIKKGLEEVKGVKGRMELFSYVKKPTFIIDYAHTPDALKKTLNAIHSHYQRYIWCIFGCGGERDQKKRPIMGAIAEKMSDKVIITNDNPRNEKEKKIIQDILNGCKNKEKILIIPDRKKAISYAYFGAKYHHIILIAGKGHETYQILGDKTIHFDEREVVNEILSE",
    "response#3": "MKLKELFSNLKITEEKNGNEDIEITGVTADSRKAAKGFLFAALPGTKVDGRAFAAKAIEAGAAAVIANPKVSGIAYDSRTVKKGDLFVCIKGFSDDGHRYLDDARQAGAVAAVVEEEVEAPDSFTVSASEVMGLLADDFYGNPSSKLKVIGITGTNGKTTSAFIIKNILQSNGLPCGLIGTIQYNFGDEILETSNTTPDSLKLHETFNQMLQKGVDSVVMEVSSHSIALHRVDGCHFDIGVFTNLTKDHIDFHGTMENYKEAKFKLFEQSHCKIFNKDDESVEIIIDKNKHPIITYGLESDANISAKEIKLDRNGTSFNVQTPKGTFSVKLKMIGKFNVYNALAAIAACHVENVSLKKIKEVLEDIEGVAGRMELFSYVKKPTFIIDYAHTPDALKKTLNAIHSHYQRYIWCIFGCGGERDQKKRPIMGAIAEKMSDKVIITNDNPRNEKEKKIIQDILNGCKNKEKILIIPDRKKAISYAYFGAKYHHIILIAGKGHEEYQIINHVKIHFDDREEAKKAIKELYE"
  },
  {
    "reference": "MPKKIHPTWYPEAKVICNGELVMTVGSTKPEIHVEVWSGNHPFYTGTQKMIDTEGRVDRFLRKYKMGDKSSKKADQK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a specific function in translation and should be localized to ribosome in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "LLKIRLKRFGE",
    "response#2": "GLKIRLKRFGE",
    "response#3": "MKKVNIRDLARFESGSVVDGEALKAKGLVKGRVDGIKLLGKGDIAYPLTVKVSHVSRSAREKIEAAGGAIE"
  },
  {
    "reference": "MQNLDHIISEAQQDFASCASIADLEQAKARYLGKSGSLTEALKGLGKLSAEERPAAGAAINVVKQQVEAALKQRRDAILQAEQERQLASETIDVTLPARTRGTGGLHPVSLTLRRVEELFRSIGFEVAEGPEIESDFYNFTALNIPEDHPARAMHDTFYVDDKHVLRTHTSPVQVHYMQDKAPPLKIIAPGRVYRVDSDATHSPMFHQVEGLWVDEHISFANLKGVVQDFLQRFFERDDLQVRFRPSFFPFTEPSAEMDMSWNGGWLEIGGCGMVHPEVFRHVGIDSEQYRGFAFGLGVERLTMLRYGVNDLRLFFENDLRFLKQFA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have ATP binding within cytoplasm to regulate phenylalanyl-tRNA aminoacylation. The designed protein sequence is ",
    "response#1": "ACPLYCGRVLKGVNAKAPTPEWMKRRLERSGIRAISALVDVTNYVMLELGQPLHAFDNTRLEGAVHARLAKPEEKLLLLNEQTIAVDADVLVIADDVKPLAMAGIMGGEESGITLETSELFLESAFFAPKAIAGRARRYGFGSDASHRFERGVDFGGARRAIERATQLILEICK",
    "response#2": "DLGVEPPEYPAPVPHFFNEFLDYVREVMIGLGFEEVRGPVLEVEFWNFDALFQAQDHPAREVHDTFYIRWEGPIEAPPEHLLEAVGRVHEEKWRYKWSREKALNPVLRTQTTAVTIRALAERGDGEYKVFTIGRVFRPEKLDPKHSMEFHQLDGIVVGPGLTFKHLLGQLEQIAKALGMTRVRFRPAYFPFTSPSVEVYAEHPKLGWVEFGGAGIFRPEVTEPLGVRKSRVLAWGWGLDRIAMILLGIDDIRELFTKDPEKLRR",
    "response#3": "KQSTSGIYSYLPLATRVLNNISKIIREEMESIDAVEILMPALQQAELWEESGRWSAYGPELMRLKDRNGREFALGPTHEEVVTSLVRDELKSYKQLPLTLFQIQSKYRDEKRPRFGLLRGREFLMKDAYSFHSDEASLDATYQDMYQAYSRIFKRVGINARPVVADSGAIGGSHTHEFMALSEIGEDTIVYSNESDYAANIEKAEVVYHPSHKHSALAELTKVETPNVKTAQEVAEYLKRPLDEIVKTMIFKIDGEFIMFLVRGHHELNEVKLKSYFGTEHVEMATPDEIVNLVDANPGSLGPIFDKDIKIYADNYLQDLNNFVVGANEDHYHYINVNIGRDFDVTEYGDFRFITQGEMLSDGSGVAQFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGVSRTLSAIVEQNNDENGIIWYNIHIPSAKTFIGRANSYLDYISKIKDKLVGLGFQEFDGPLVETEFFNNDALFMPQFHPSRDIKDVYYISDPSMQESLPEPYFSNVKLAHETGYATGSRGWRYSFSEDLSKRLVLRTHGTVLSAKQLINAKNPSRYFGVIRCFRYDQVDATHGVDFYQTEGIVIEDGVSIKTLLGLLEIFAKELAGATEIKYVPAYFPFTEPSIEIHVKHPVLGWFELGGSGIFRPEVTKPLGIDLPVIAWGIGIDRMALMHLGLNDLRDLFTYDV"
  },
  {
    "reference": "MIKVAVTGALGRMGSNIIKTITQQEDMKVVCAFEVPNHPKKGEDVGELIGIGKIGVPLSTADELDKVLKETKPDVLVDFTIAHACVENVKIAAKNGVNLVIGTTGFTEEQKAEIEKAIKENNVAAVISQNFAIGVNIFFKTLEFLAKKLGDYDIEIIEMHHRYKKDAPSGTALRAAEIIKANRGIESVFVYGRYGMTGERKKEEIGIHALRGGDVVGDHTVIFAGDGERIELTHRASSRQAFVNGVILAIRYIADKKEGIYNTFDVLGLNEIKF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.2. The Proton donor/acceptor active site of the designed protein must be optimized for substrate binding and specificity.3. Ideal protein should have a binding affinity for key enzymes in the Amino-acid biosynthesis pathway to enhance the overall efficiency of the pathway.4. The protein should be able to bind (S)-2,3,4,5-tetrahydrodipicolinate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MGQEVVKMIQSSDNLTLVRGISRSQYGKDVGEVAGIGPIGVTFTDSLDEVLAKDRPDVLVDFTVPDVVKKNMELAIDAGVRPVVGTSGLSEEEIQELQELAKKKGVGAIIAPNAVLTMKFAELAAPYFESAEIIEMHHPNKVDAPSGTAVATAKAISQGRNGKASPDATQIDPQGARGAVYDGVSVHSVRLRGLNAHQEVLFGNPGEQLLVRTDCFQRVSFMPGVMLACRKVADLEGLTVGLDKVL",
    "response#2": "MGQEVVKMIQSSDNLTLVRGISRSQYGKDVGEVAGIGPIGVTFTDSLDEVLAKDRPDVLVDFTVPDVVKKNMELAIDAGVRPVVGTSGLSEEEIQELQELAKKKGVGAIIAPNARLTMDAVKAGGKNAVLLMKFAKEAAKYFPDVEIIEMHHADKKDAPSGTALATAKMIAENRPAHQTAPDEVETLENVRGGDYEGIKIHSVRLPGYVAHEQVLFGGTGEALTIRQDSFDRQSFMSGVKVALEKVDQLNELVVGLENILGLD",
    "response#3": "MGQEVVKMIQSSDNLTLVRGISRSQYGKDVGEVAGIGPIGVTFTDSLDEVLAKDRPDVLVDFTVPDVVKKNMELAIDAGVRPVVGTSGLSEEEIQELQELAKKKGVGAIIAPNARLSWDNAVKAAGGAVLTMVFSKQAARFFESAEVIELHHPNKLDAPSGTAIHTAQGIAAARKEAGMDAQPDATEQALEGSRGASVDGIPVHAVRMSGMVAHEQVIFGTQGQTLTIKQDSYDRNSFAPGVLVGVRNIAQHPGLVVGLEHYLG"
  },
  {
    "reference": "MKKYSIAIIGATGVVGEELLVVLKKLNFPVENILPLASKNSAGSYIEFDDKQYKVLQLTDKIFTEHKVDIAFFSAGGGISKKYSKYAVNSGAVVIDNTSHFRMDTDIPLIVPEVNPQDIVMWKNKGIIANPNCSTIQMVQALKPLDDLYGISRVDVATYQAVSGAGKDGMKELVTQLQDFFAFKLHDSKKEIFVHQIALNVIPQVDIAMPSGYTKEEEKMIYETRKILHKDIKVSATCVRVPVLRSHSEAVTVTFGDKIDIDIKKCQTVLKNFKNMKLIDDIKNNLYPMPTTATDTDNTYVGRLRKDIFSPNILHFFNVADQVRVGAATNSVRIALLWIKEN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein with the reaction L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH, the Proton acceptor, Acyl-thioester intermediate must be optimized to ensure efficient catalysis.2. The phosphate, substrate, NADP(+) binding site should be located in a region of the protein that is accessible to the ligand.3. A protein should be able to manipulate the activity of enzymes in the L-threonine from L-aspartate: step 2/5, Amino-acid biosynthesis, L-threonine biosynthesis pathway, either by activating or inhibiting their function.4. The protein contains novel Semialdehyde dehydrogenase NAD-binding domains that confer a unique function or activity.5. The designed protein must possess aspartate-semialdehyde dehydrogenase activity, NADP binding, NAD binding to support lysine biosynthetic process via diaminopimelate, 'de novo' L-methionine biosynthetic process, isoleucine biosynthetic process, threonine biosynthetic process under certain conditions.6. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate. The designed protein sequence is ",
    "response#1": "MARIAVVGATGNVGREMLNILAEREFPLADVAAVASSRSQGTKIDFGDSGRMLTVQSLEHFDPTGWDMALFAIGSEGSRLHAPRFAAAGCTVIDNASEYRMDPDVPLIVPEVNPQDIDGAKPKGIIANPNCTTMAAMPVLKPLHDLATIKRVVVATYQSVSGAGKEGVDELWDQTKGVFVNDKPEPRKFTKQIAFNVIPHIDVFMEDGYTKEEWKMVAETKKILDPKIKLTATCVRVPVFVGHSEAVNLEFANPISADEAREALREAPGISVIDKREAGGYATPVEAAGEYDTYVSRIREDVTVENGLSLWVVSDNLRKGAALNTIQIAELLV",
    "response#2": "TVGLVGWRGMVGSVLMERMRVEDDFKHFEYFFFSTSQAGRKGPALGQNDGLVEDAYNIDRLSQMNIVVSCQGGDYTQKCHGQLRQNGWKGYWIDAASTLRMQSDSVIILDPVNRPVIDEALAKGGRNWIGGNCTVSCMLMGVGLKPLHDLATIKRVVVATYQSVSGAGKEGVDELWDQTKGVFVNDKPEPRKFTKQIAFNVIPHIDVFMEDGYTKEEWKMVAETKKILDPKIKLTATCVRVPVFVGHSEAVNLEFANPISADEAREALREAPGISVIDKREAGGYATPVEAAGEYDTYVSRIREDVTVENGLSLWVVSDNLRWGAAEPLRRMLRILLGH",
    "response#3": "MSSNVAIMGATGAVGQVFLEILQQRNFPVGELRLLASSRSAGKTVDFRGDTLTIQELSKDSFEGIDLVLSSASGSLSREFVPAAVEAGAVVVDNTSAFRMDPQVPLVVPEVNSADLDDYSTRNRIVDGICVRVGAMRCHLAPIKAKYGLTRVIVSTYQAASGAGQSAWEELVAETTAHNAGQEMHANILPVKNAEHHYPLAYNLLPQIDVFEDDGYTHEEWKMIHETKKIMFGDKNSKAVKVTGTAVRVPVPIGHGEAVYFETEAGTGATSGDIQSILSKAPGVVLEDNPKEQLYPQPINAEGKRETFVGRVRPDLENDDSFHMWVVSDNLLGNATANWQEGLAPIKAKYGLTRVIVSTYQAASGAGQSAWEELVAETTAHNAGQEMHANILPVKNAEHHYPLAYNLLPQIDVFEDDGYTHEEWKMIHETKKIMFGDKNSKAVKVTGTAVRVPVPIGHGEAVYFETEAGTGATSGDIQSILSKAPGVVLEDNPKEQLYPQPINAEGKRETFVGRVRPDLENDDSFHMWVVSDNLLKG"
  },
  {
    "reference": "MKILTAAQLAEADQATLKSQNISSTDLMERVSTLVFNKIHERLNGAPVPVKIFCGIGNNGGDGLAIARHMIQHGYHVTTYVTNCSKKRSKDFLVNYDKVKEVTKDWPILLDCEEDIPEISPKDIVIDAIFGIGLNRPVKGWMASLFNRTNDSGAFTVSIDMPSGLFSDTAQPADSAVIQADHTFTFNSPKLSFFLEDTGNYTGTFEVLNIGLDPTFIHQADPAAMLITRESAQNIYRPRKKFSHKGDYGHVLVMAGSKGKIGAAVLASTAAINSGAGKVTAYVPSCGNDILQSSVPEVMTIADSGVDQLNDFKTELEKYILCVGPGIGTSDEVVSAFAKAIKLQSSPVLIDADGINILAANIELLKDVPAKSILTPHDGELERLLGGWDSSMERLDKAREFSMQHDVILVLKGAHSITVYKDHMYINDSGNAGMATAGSGDVLSGIISSFMAQSYDPLMAAVFGAYIHGASGDIAAQTYAHEGLRAGIISNFVGPAILQLFRQENK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a high affinity for K(+).2. The protein should have a fast turnover rate for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.3. The designed protein should be optimized for kinase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, metal ion binding activity in order to improve nicotinamide nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "VWTEEDVRGTWPAAKDYTHKGTFGTGLLIAGSRQMPGCAILAGTGAMRAGVGKLVMATEKTVADSCVISLPEATYVDREDSQWRKDPSISKIAAVAIGPGLKPDDSLENLITDFLEESEFPVILDAGALSERSYPKGRKSPVILTPHPGEMARITGKTVAEVNENRIQLASDYAISHHVTVVLKGMNTVIAFPDGTGFVNVTGNRGLSKGGTGDTLTGILLGMASQGYQPSMAANAVYLHGKTAELYAEEKAPSSMLASDIMQIWAAQTRAADQATIQAERITSTDLMERAAMALSEWLLDRLARQAVHIFCGPGNNGGDGLVIARHLHHAGHAVHVWLLPANAYSSDFTANRQRLPADVPCAELDVQRLPPLPAGAVLVDALFGTGLTRVLTDDAATLVHYINQAHLPVVAVDIPSGLFADAPQPADSPVVAARFTVSFELPKLAFLLPQNAQWVGEWHLLPISLE",
    "response#2": "VGVVAGSARYPGAAVLAVSGALRGGAGAVRYVGPGGEAVLARFPETLVHEGPPSGAGRVQAWAVGPGLGDGSWAAAAVADVLAADVPVLVDADGLRLLAERTVRERTAPTLLTPHAGEAAALLGVDREEVEAGRLAAVRELAARYGATVLLKGSTTLVAEPASGSASGSASGSASEPGAAPPVRVNPTGTPWLATAGSGDVLSGLAGSLLAAGLPARDAASAAAYLHGLSARLASRR",
    "response#3": "VGIAAGSARYPGAAVLAVAGALRGGAGAVRYVGHAGEAVIARFPEALVHAGPPAKAGRVQAWVVGPGLGDETGAVAEILASDVPVLVDADGLRPLDASAVRRRSAPTLLTPHAGEAAALLGVDRAEVEAARLASVRELASRYGATVLLKGSTTLVAAPDGALPVRANPMGTPWLATAGSGDVLAGLTGSLLAAGLGAYDAGSVAAYLHGLAARHASAKGVPG"
  },
  {
    "reference": "MAKKSLIAKQKKHQKFAVREYTRCVRCGRPHAVNRKFGVCRLCFRDLAYAGAIPGIKKASW",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The presence of Zn(2+) is necessary for the protein to function in the desired environment.2. The designed protein should have rRNA binding, zinc ion binding within ribosome to regulate translation. The designed protein sequence is ",
    "response#1": "GCNFCGKSIEPGTGKKFVKKDGSVMFICSSKCEKNYKLKF",
    "response#2": "MAVPKKRTSKAKKNMRRS",
    "response#3": "MFGVS"
  },
  {
    "reference": "MSKKPGKSAAKRTVNMLKRLASVSPSRGRRTIRRMLDVRGAPRLILALMAFFRFAAIKPTLGLKKRWRSVNKTVAVKHLTNFKKELTTMLDSVNKRKEKKKSFSTALLWITMITAVAGLKISSHRDRPLLMVNKTDVSDAIPVPSVKGTNMCTIRALDVGYTCAYDTTYECPHLEVTMDPEDIDCWCTLESVYVNYGLCKQNHHVRRGRRAINIPHHGESHLENRATPWMDTTKTTKYLTKVENWVIRNPGYALVALATAWMLGSNTPQRVVFMIMMMLIAPAYSLNCLGISNRDFVEGLSGGTWVDIVLEGGSCVTVMAKDKPTLDIKLIRMEAKDLATVRSYCYQATVTDSSTEARCPTMGEAHNSKSLDASYVCKSSYVDRGWGNGCGLFGKGSIQTCVKFSCPGKATGKSIQRENLNYDVAVYVHGPISAAAHGNYTAQLTGKYAAKFSITPSAPTYTANLGEYGEATMECEPRAALDIDNYYVMSLNNKHWLVNRDWFHDLDLPWTGPATESWKNRESLIEFEEPHATRQTVVALGNQEGALHTALAGAIPVEVSSTTLTLNSGHLKCRLKLDKLKIKGTTYAMCKGTFAFAQTPVDTGHGTIVAELTYTGTDGPCKIPISMTADLRDMTPIGRLVTVNPIIPSSAKSQKILVELEPPFGSSFILVGQENNQIKYQWHKTGSTIGNALKTTWKGAQRFAVLGDTAWDFGSVGGIFNSIGKTIHGVFGTAFRSLFGGMSWVTQALMGALLLWLGISARERTVSL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should contain one or more mRNA cap 0-1 NS5-type MT, Helicase ATP-binding, RdRp catalytic, Peptidase S7 domains that are essential for its function.2. Incorporating a DEAH box motif is critical for the successful design of the protein.3. The Cytoplasmic, Lumenal, Extracellular topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.4. A protein that contributes to viral RNA genome replication, fusion of virus membrane with host endosome membrane by functioning in viral capsid, host cell nucleus, membrane, viral envelope with double-stranded RNA binding, metal ion binding.5. The designed protein is the optimization of the For 2'-O-MTase activity to achieve high catalytic efficiency for a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside   in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-   triphosphoguanosine)-(2'-O-methyl-ribonucleoside) in mRNA + H(+) + S-   adenosyl-L-homocysteine.6. The designed protein should have at least Helical; Note=Signal for NS4B transmembrane domains to ensure proper localization.7. The designed protein should have a binding site that is specific to the target S-adenosyl-L-methionine ligand. The designed protein sequence is ",
    "response#1": "KRSPIALPDIVPPPLQLFIAVAAAEEGHPNNTSYLANYNLYATKAPPGVQAIKPGISLTDFEALFGPQPTLRAIRNLVCEKDPFWVKSKDAFAQKSNSLNFSDAVLRAMIRHGPAKVASLAALALFNRLVKIDQEDLKQLALSAAVNHAFDHTTGQAIAQAKSVTLLHKDSYLTLNIEPFAKTFTNLLIAQATTLNAAMKYNDYLLYPGAAARKVNGDYIPIATKPIPCNITGKGTFEDVNLLPDYQALAVDQVEGTLVTIMDIPYVKTPTTPPVYYQRVKGGVLAVSLKVQGTSNKKLNVVFHTEPSEVLMAFIQLQQFLNNTNNSWVEVTEAQEFQVNPTTVVKIGTSQPGDVVVKTEQEYLDCFNDPNTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQLDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFPLNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTGDFVKATCEFCGTENLTKEGATTCGYLPQNAVVKIYCPACHNSEVGPEHSLAEYHNESGLKTILRKGGRTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNLLEILQKFSFAKNPADTGHGTVVIELSYSGSDGPCKIPIVSVASLNDMTPVGRLVTVNPFVATSSANSKVLVEMEPPFGDSYIVVGRGDKQINHHWHGTGRFSIENVLSYAQKLKYLLPQYRNFSFSLKEKELNVGRTFGKLPYPTRNVQTLCEALLADGLAKAFPSNMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGPSSYRGHMGGIEGLQQKLWTSISCAQISLVEIKTGFKLRSAVMGDNQCITVLSVFPLETDADEQEQSAED",
    "response#2": "TRYVXXXXXXXXXXPLECIKDLLARAGKASCTLSEQLDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDIFNGECPNFVFPLNSIIKTIXXXXXXXXXXXXXXXXXXXXXXXXXNECNQMCLSTLMKCDHCGETSWQTGDFVKATCEFCGTENLTKEGATTCGYLPQNAVVKIYCPACHNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNLLEILQYGMCDSAFTFSKNPADTGHGTVIVELQYTGSNGPCRVPISVTANLMDLTPVGRLVTVNPFISTGGANNKVMIEVEPPFGDSYIVVGRGTTQINYHWHGRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVVTLPSHSTRKLQTRSQTXLESREYTKHLIRVENWIFRNPGFALAAAAIAWLLGSSTSQKVIYLVMILLIAPAYHKVNINIVGDFKLNEEIAIILASFSASTSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVRVLQKAAITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKESVQTFFKLVNKFLA",
    "response#3": "KVNINIVGDFKLNEEIAIILASFSASTSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVRVLQKAAITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKESVQTFFKLVNKFLACLGTSNRDFVEGASGATWIDLVLEGGSCVTVMAPEKPTLDFKVMKMEATELATVREYCYEATLDTLSTVARCPTTGEAHNTKRSDPTFVCKRDVVDRGWGNGCGLFGKGSIDTCAKFTCKNKATGKTILRENIKYEVAIFVHGSTDSTSHGNYFEQIGKNQAARFTISPQAPSFTANMGEYGTVTIDCEARSGINTEDYYVFTVKEKSWLVNRDWFHDLNLPWTSPATTDWRNRETLVEFEEPHATKQTVVALGSQEGALHTALAGAIPATVSSSTLTLQSGHLKCRAKLDKVKIKKFSFAKNPADTGHGTVVIELSYSGSDGPCKIPIVSVASLNDMTPVGRLVTVNPFVATSSANSKVLVEMEPPFGDSYIVVGRGDKQINHHWHCYTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQLDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFPLNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTGDFVKATCEFCGTENLTKEGATTCGYLPQNAVVKIYCPACHNSEVGPEHSLAEYHNESGLKTILRKGGRTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNLLEILQKEKVNINIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGNFKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVRVLQKAAITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKESVQTFFKLVNKFLALCADSIIIGGAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMPLKAPKEIIFLEGETLPTEVLTEEVVLKTGDLQPL"
  },
  {
    "reference": "MKKVKTVGKNTKRFITGIVHIRATFNNTFVNVTDVCGNTLYQTSVGACGFSGSRKSTPYAAGKVADSAAKKVIERFGMKVVSVIIRGPGFGAEAAVKALRNCGLTVTSIADKTAIPHNGCRLRKKRRV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design must be able to perform rRNA binding in order to contribute to translation.2. For general function, the protein need meet that Forms part of the Shine- Dalgarno cleft in the 70S ribosome.; Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA The designed protein sequence is ",
    "response#1": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTGE",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTF",
    "response#3": "MSRRHAAEKREVLPDAKYGDLVLTKFMNNLMIDGKKSVAETIVYNALTRVETKIKRAPIEVFHEALDNIQPSVEVRSRRVGGATYQVPVEVRPERRQALAIRWLIKAARSRNENTMEERLAGELLDAVQSRGTAVKKREDTHKMADANRAFAHYRW"
  },
  {
    "reference": "MNRYMKLNNQVLIGSSKSQLESLAVSLGEKPYRGXQLFDWXYRQTIDDIEXMTDLPVFFRKRIKNFTTLHPLTQIKNLDSNTKLTKKFLFKLQNGNQIESVFMEQKERITICISTQVGCALDCDFCATAKMGFVQNLSPGEIIDQFLQLKRLSRKPITNIVFMGMGEPFLNYKNVIAAAELLHNPEGINIGAKRITISTAGXIPKIKQFTXDGHKXKLAVSLNGTTQXQRLKTMPVTXKHPXDELLVTVKDYVQKNKTRVTFEYVLMKDINDSMEDAKRLKKMLSSINCKLNIIPYNDIGGVYLRPNNXKIQFFLSGLDKAPFPVTVRWSKGVKIDAGCGQLVTRIER",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must bind to [4Fe-4S] cluster in order to perform its enzymatic function.2. A protein that localizes to cytoplasm and can perform tRNA (adenine-C2-)-methyltransferase activity, rRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding with high specificity and efficiency.3. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. The designed protein sequence is ",
    "response#1": "DGDRATLCVSSQVGCKMGCAFCQTGKQGFDGNLTAGDILNQIYALPEREQLTNIVMMGQGEPMDNVENVLAAIEVMTASWGYGWSPKRITVSTVGLRKGLRKFLNGCDCHLAVSLHSPIHEQRLNMMPAEGAMTIDEVVETLREYDFSHQRRLSFEYIVFKGLNDSRMHAKEIVKLLSGLDCRINLIRFHEIPGVDLPSSDEEGMLRLRDYLTAHGFFTTIP",
    "response#2": "ISTQAGCPLKCAFCLTGIAGYKRNLKTWEILGQVAVVMEEGPREHLPWNVVVMGMGEPLLNYEATVSALRVLMDPEGFAVAPRKLTLSTVGILPALEKLMQEPVRPNLAISLHAPNQALRRVLMPIEEKYALADVMTAALRYPIPRGGLVTFEYVLLRGVNDRPPAYK",
    "response#3": "DGDRATLCVSSQVGCKMGCAFCQTGKQGFDGNLTAGDILNQIYALPEREQLTNIVMMGQGEPMDNVENVLAAIEVMTASWGYGWSPKRITVSTVGLRKGLRKFLNGCDCHLAVSLHSPIHEQRLNMMPAEGAMTIDEVVETLREYDFSHQRRLSFEYIVFKGLNDSRMHAKEIVKLLSGLDCRINLIRFHEIPGVDLPSSDEEGMLRLRDYLTAHGFFTTIP"
  },
  {
    "reference": "MALQIAKRLLRCRADSVASSVRFFDRTFTSESNSNLIRATLFPGDGIGPEIAESVRQIFKVAEVPIEWEEHYVGTEVDPRTNSFLTWESLESVRRNKVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVSMLRHLELHDKADRIQDAILKTIAGGKVPNWRPWRHCYNN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have a high specificity for NAD(+) binding, with minimal off-target effects.2. The protein must be engineered to have a high affinity for Mn(2+) in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "GEVYLIGAGPGDPDLMTFKALRLLQRADVVLYDRLVAPEIVELCRKDAERIFVGKARSNHAVPQQDINALLVEHAKQGRKVARLKGGDPFIFGRGGEEIEQLANESIPFQVVPGVTAAAGCASYAGIPLTHRDYAQSVRFVTGHLKDNSINLPWAELVHGHQTIVFYMGLVGLPVICEQLIAHGRSADTPIALVQQGTTRHQKVVVATLATMVEEVAKNPVKAPTLIIVGEVVSLH",
    "response#2": "PTAMKYERGRSLVVAGSDRYPGAAHLALRGAMASGCGCVQAVVPPCLQSNLWQVLPEVMQLEEGVIPDRLDAVLVGPGLGNPSRWWNQWSEQLRTVAGLLVLDADGINALASSPEGWRWLLQRQGPTWLTPHAAEFARLFPDCGEGDALERVIAAARCSGCCILLKGAHSVLADSSGAAVVLTGTSPWVARTGLGDLLAGFVTGWGAQAVAAHQQPGLESFVASTALHGLAARRR",
    "response#3": "IGAGPGDPDLMTFKALRLLQRADVVLYDRLVAPEIVELCRKDAERIFVGKARSNHAVPQQDINALLVEHAKQGRKVARLKGGDPFIFGRGGEEIEQLANESIPFQVVPGVTAAAGCASYAGIPLTHRDYAQSVRFVTGHLKDNSINLPWAELVHGHQTIVFYMGLVGLPVICEQLIAHGRSADTPIALVQQGTTRHQKVVVATLATMVEEVAKNPVKAPTLIIVGEVAALMEKVGLAMASWLLARSDLLRNGVVVLVGPGHNGGDGLVVARELHLAGIEVSLWCPLAIRKQLTADHLRHGEWLGLRRLRQDPDPADAALWLDAIFGLGQSRALPESFEDLFRRRQQLQPGALISLDVPSGLCSDHGTVLGEQAACASATLSVGWMKRS"
  },
  {
    "reference": "MAMSSYMVNSYVDPKFPPCEEYLQSSYLGEQGAEYYGASQGSDFQHQGLYPRSNYSEQPFWLAGAQGSAVQPRGHGQEQSAPPSHFPGQAEHCPPPPCPIPSCGQQPALKAPHGSAVKQPAVVYPWMKKVHVNSVNPNYSGGEPKRSRTAYTRQQVLELEKEFHFNRYLTRRRRIEIAHTLCLSERQIKIWFQNRRMKWKKDHKLPNTKGRSSSSASNPHLQTVPKDHQTDLTTL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be optimized for nucleus-specific folding and stability, while maintaining DNA-binding transcription factor activity, RNA polymerase II-specific.2. The protein must exhibit the following characteristics: The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'.; Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.3. The protein design must feature the Antp-type hexapeptide motif. The designed protein sequence is ",
    "response#1": "TAATGAIAHALCLTERQIKIWFQNRRMKWR",
    "response#2": "ATAAAGA",
    "response#3": "TTRLRKPRFSFEENQILIREVRAHYPQLYGAQSRRVSVAERRRVWDGIAAKINGITSWKRTGQEVQKRWNDFL"
  },
  {
    "reference": "MTDLAGKHIVLGLSGGIACYKSAELCRLLVKAGATVQVLMTEAAAQFITPVTMQALSNRPVVISQWDAREPNNMPHINLGREADAILVAPASADLLAQLAQGRAADLLTLTCLARPLERAPLLLAPAMNREMWAHPATQRNIAQCQADGATVLPVGQGEQACGETGDGRMLEPAQLLEALVASFQPKRLAGHQVLVTAGPTFEAIDPVRGITNHSSGKMGFAIARAAREAGAEVTLVAGPVHLPTPLGVRRVNVQSALQMQAAVQAEVGAATIFIAAAAVADWRPASAAEHKIKKDGSGLPPALHFVENPDILAGVAADPRASRGELYCVGFAAESQDLLANAQAKRLRKRVPLLVGNIGPATFGRDDNALLLVDAQGAQELPHGSKTELARQLVAEIARRLPVAG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have a high affinity for CTP.2. The designed protein should interact with enzymes and substrates within the CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis pathway to enhance or regulate its activity.3. The protein must have high specificity towards phosphopantothenoylcysteine decarboxylase activity, phosphopantothenate--cysteine ligase activity, metal ion binding.4. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal domain.5. The protein should be designed to have high stability and activity towards H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2. The designed protein sequence is ",
    "response#1": "MNIVLGVTGGIAAYKAAHVVRLLKEAGHSVRVVPTESALCFVGAPTWEALSGQPVQTDVFTRVEEVEHVLIGQQADLVLIAPATADSLARIRAGMADDLLTATVLTTTAPVVVAPAMHTEMWFNPATVDNVAVLRARGVHVLEPASGRLTGADSGPGRLPEPQDIVDFALDVLRPSDLSGLKVIVTAGGTKEAIDPVRYITNHSSGKQGYALAQAALDSGAEEVVLISTGVDLPIPYGARIVCAPSAEEMLDAIRTHLDGTHVLLMAAAVADFRPSQAATQKIKKSDDPDEATSLQLVRTSDILREVRTARDQTGWPWIVVGFAAESQDLIANAQRKLERKGLDLIVANDITASDAGFGSDMNRVTLIRADGATQELPLMTKKELAEAIVDRVVTLMGG",
    "response#2": "MNIVLGVTGGIAAYKAAHVVRLLKEAGHSVRVVPTESALCFVGAPTWEALSGQPVQTDVFTRVEEVEHVLIGQQADLVLIAPATADSLARIRAGMADDLLTATVLTTTAPVVVAPAMHTEMWFNPATVDNVAVLRARGVHVLEPASGRLTGADSGPGRLPEPQDIVDFALEVLGGPRPLADRTVVVTAGPTREPLDPVRFLSNHSSGKMGYALAEVARDRGARTILISGPTCLPAPYGVARVDVGTAVEMLRAVEEATASADVLVMCAAVADYRPRVVATEKLKKRQELFVLELEPTEDILATINRPGLVKVGFAAETERLLEHATEKLERKRLHLLVVNDARKTMGAETAQVTLLWADGSTELPLMSKREIAEQLLDLIAERWSAR",
    "response#3": "MNIVLGVTGGIAAYKAAHVVRLLKEAGHSVRVVPTESALCFVGAPTWEALSGQPVQTDVFTRVEEVEHVLIGQQADLVLIAPATADSLARIRAGMADDLLTATVLTTTAPVVVAPAMHTEMWFNPATVDNVAVLRARGVHVLEPASGRLTGADSGPGRLPEPQDIVDFAETLLRRADLLADRTVVVTAGPTREPLDPVRFLSNHSSGKMGYALAEVARDRGARTILISGPTCLPAPYGVARVDVGTAVEMLRAVEEATASADVLVMCAAVADYRPRVVATEKLKKRQELFVLELEPTEDILATINRPGLVKVGFAAETERLLEHATEKLERKRLHLLVVNDARKTMGAETAQVTLIRPDGTRSWEDLPKAEVAARLAERIAAEWSA"
  },
  {
    "reference": "MTIRNVTRGLRCTLAFRTLPNALPVQASLCRVDCALLSTTCARRKSGLKADGSRADQAEAGASQSLDKQSRTLDSVRDDTLSDPYPLPFSPDIVELNSVKTRVEAGLESKWSGVNLASSQQTLNHIDAPMRVPGRDSEARETKIARLIYQTRKRGTLETDLLLSTFAKKELKNLPDAELDEFDRLLDEPDWDIFYWCTQRKPIPARWAHSFNTAGKLGHRLVAHTRNDEKAVRWMPEL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for stability and expression in mitochondrion, mitochondrial matrix where it will participate in protein-FAD linkage, mitochondrial respiratory chain complex II assembly, mitochondrial electron transport, succinate to ubiquinone, tricarboxylic acid cycle.2. The protein must exhibit the following characteristics: Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit of the SDH catalytic dimer.; Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. The designed protein sequence is ",
    "response#1": "MSILSGKKQYTD",
    "response#2": "METRVLPLLLLLLAACCHGVASLGRXH",
    "response#3": "MPIGVPKVPFRSPGEADPSKCTEIYDQLYRERLLFLGQEVDSEISNQLISLMIYLSIEDDTRDIYLFINSPGGWVIPGIAIYDTMQFVRPDVHTICVGLAASIASFILVGGEITKXVXXAHXMVYXPSGGARGQATDIVXEAEXIMKTRKMINEIYVKHTGKPYEVVEQDLERDVFMSAKEAQAYGLIDSVITQRPSS"
  },
  {
    "reference": "MKILFIISSSIAAKKCESIIKLLVDNKIEIKIILTENSKKMINISKLKKITNGKIYTDNSEKNNKMLHIDLSRKANLVVVCPATANTIAKFANGYGDNLASTTLLASNKQIIFVPAMNVQMWNNKINQKNIKFLKDNGIEFVGPQYGKLYCGEKGFGRLANEKLIVNTIIKNLKLTKIFKGKQCLVTAGPTIEEIDPIRYISNYSSGKQGYEIAEQLVLSGAKVTLISGPTNLSAPNKVKLIKIKTADDMFKEVKKIKNIDIGIFASAVSDFKPKNKFNSKINKNDIKKIEVKKNIDILKYIGTLRKNRPKLVIGFAAETENIQRAVSKLNEKKCDLIVYNNINNSNKVFGSNYNKITLITKYKKFSFPKMSKAKCAKKIISTVNNLYFN",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should contain one or more Flavoprotein domains that are essential for its function.2. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..3. The designed protein must possess phosphopantothenate--cysteine ligase activity to support coenzyme A biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "MTGGIAMYKAVQVVRNLEKEGHEVRVIMTKNAERFVTSNTLAALTKYPVLDNLWSKENEASVPHVHLARWTELALVVPATANFIAKMANGIADDAASTTMLATSAPILIVPAMNDQMWNNPATQRNLQFLKNNQVQIMEPAVGMLAEGYAAKGRMPEPDQISAWVEDHLAGRHVVVSAGGTRERLDPVRFLGNRSSGRQGYALARAAAARGAEVTLIAANVSLGDPAGVKVVRVESTAELRDAVLTAAATADAVVMAAAPADFRPTAASDTKIKKSDDGSAPSITLQQNPDILKEISQHRVRPGAVIVGFAAETGDATGSVLDLARAKLARKGCDLLVVNDVSGGAVFGSTDNEAVILGADGSRELTGKNVLVTAGPTMEAIDPVRFISNHSTGKMGYAVAKTAARRGAKVTLVSGPVNLLPPRFVETVQIESAAQMYEEVIKRADEQDIIIKAAAVADYTPENTADEKIKKKDGGEMSIPLKRTKDILKTLGENKKENQFICGFSMETQNMLENSKKKLEKKNVDMIVANNLKVAGAGFGTDTNIVTLITADEAEELPLMSKDEVAERILDRVAPIL",
    "response#2": "KHILLIIGGGIAAFKSLDLIRRLRERGAIVTPVLTRAGEEFVTPLSVAAIAGQKLYRDLFDLGDEAEMGHIQLSRVADLIVVAPATADLMAKMAGGLANDLASTLLLATDTPVLIVPAMNVRMWDHPATQRNLKTLRGDGINVIGPNDGDMACGEFGPGRMSEPLEIVAAIEAALRPPVTLENRKVLITGGSTAESLDPIRILTNRASGKTGRELALEAYRSGADVTLVHRDRLRLVGIREVFAESAAEMTEAVLSELDTGYDVLISSAAIADYTVEPSPEKIKSGGEFALKLKTTRKLIRECREKYPELVIIGFKAETGVGREELLKRATATLEGTKLDMIAANDVGKGGMGTEENELYLQGRSVLVTAGPTREPIDAVRFIANRSSGRMGFAVAGAAAAAGATVTLIAGPSEQPTPPGVERIDVETATEMHAATLERAGDVELFIAAAAVADYRVAQPADHKVRKSDAPPTLSLVRNPDIVGDVAALPTAPFTVGFAAETHDVIDHARAKLTAKGLDMIAANRVGEGLGFEVSDNASALVLLDGAVSELPVTSKRVLARKLVELVADRLES",
    "response#3": "MTGGIAMYKAVQVVRNLEKEGHEVRVIMTKNAERFVTSNTLAALTKYPVLDNLWSKENEASVPHVHLARWTELALVVPATANFIAKMANGIADDAASTTMLATSAPILIVPAMNDQMWNNPATQRNLQFLKNNQVQIMEPAVGMLAEGYAAKGRMPEPDQISAWVEDHLEGLRVVVSAGGTREPLDPVRYLGNRSSGKQGYALARVAAQRGAEVTLVAAHTVALPDPAGARLVPVSTAEELRAAMHRAAERADVVVMAAAVADFRPDQLAAHKIKKRDGEPDPTITLARNPDILAELVAGRRAGQTIVGFAAETGDERGGVLDHARTKFKRKGSDLLVVNAVGQGRAFEVEDNAGWLLDADGAQESLPMLPKAELASALVRRVVAML"
  },
  {
    "reference": "MASTERNFLLLSLVVSALSGLVHRSDAAEISFGSCTPQQSDERGQCVHITSCPYLANLLMVEPKTPAQRILLSKSQCGLDNRVEGLVNRILVCCPQSMRGNIMDSEPTPSTRDALQQGDVLPGNDVCGFLFADRIFGGTNTTLWEFPWMVLLQYKKLFSETYTFNCGGALLNSRYVLTAGHCLASRELDKSGAVLHSVRLGEWDTRTDPDCTTQMNGQRICAPKHIDIEVEKGIIHEMYAPNSVDQRNDIALVRLKRIVSYTDYVRPICLPTDGLVQNNFVDYGMDVAGWGLTENMQPSAIKLKITVNVWNLTSCQEKYSSFKVKLDDSQMCAGGQLGVDTCGGDSGGPLMVPISTGGRDVFYIAGVTSYGTKPCGLKGWPGVYTRTGAFIDWIKQKLEP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein contains novel Peptidase S1 domains that confer a unique function or activity.2. The protein must exhibit the following characteristics: Acts downstream of pathogen recognition receptors PGRP-SA and GNBP1 and protease grass in response to Gram-positive bacterial infection.3. The designed protein should be involved in positive regulation of antimicrobial peptide production, defense response to Gram-positive bacterium by localizing to extracellular region and exhibiting metal ion binding, serine hydrolase activity. The designed protein sequence is ",
    "response#1": "IIGGREARPHSYPYMAFLLIQSPEGLSACGGFLVREDFVLTAAHCLGSSINVTLGAHNIQMRERTQQLITVLRAIRHPDYNPQNIRNDIMLLQLRRRARRSGSVKPVALPQASKKLQPGDLCTVAGWGRVSQSRGTNVLQEVQLRVQMDQMCANRFQFYNSQTQICVGNPRERKSAFRGDSGGPLVCSNVAQGIVSYGSNNGNPPAVFTKIQSFMPWIKRTMA",
    "response#2": "IIGGREARPHSYPYMAFLLIQSPEGLSACGGFLVREDFVLTAAHCLGSSINVTLGAHNIQMRERTQQLITVLRAIRHPDYNPQNIRNDIMLLQLRRRARRSGSVKPVALPQASKKLQPGDLCTVAGWGRVSQSRGTNVLQEVQLRVQMDQMCANRFQFYNSQTQICVGNPRERKSAFRGDSGGPLVCSNVAQGIVSYGSNNGNPPAVFTKIQSFMPWL",
    "response#3": "IIGGREARPHSYPYMAFLLIQSPEGLSACGGFLVREDFVLTAAHCLGSSINVTLGAHNIQMRERTQQLITVLRAIRHPDYNPQNIRNDIMLLQLRRRARRSGSVKPVALPQASKKLQPGDLCTVAGWGRVSQSRGTNVLQEVQLRVQMDQMCANRFQFYNSQTQICVGNPRERKSAFRGDSGGPLVCSNVAQGIVSYGSNNGNPPAVFTKIQSFMPWIKRTMV"
  },
  {
    "reference": "MSSLSSAPFGRVVTAMVTPFAADGSLDLPLAARLAQHLVAHGSDGLLVCGTTGESPTLSWDEQLALIKTVLDAVGEKVPVLAGTGSNSTAEAVEATREAAALGAHGALVVVPYYNRPPQEGLEAHFRAVAAAAPELPLMLYNIPGRTGCSLHPATTARLLDCDNVVAYKAASGTTSEVSALRLACGSRLAIYSGDDGLTLPMLAAGAVGVVSVTSHLAGVELSAMIDHYMAGRHAEALALHERLLPLMEGLFCTSNPTPVKAALELQGWPVGAPRLPLMPADSSVRDTLSTLMAALRPT",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA)..2. We require a protein that can specifically bind to the metabolites produced in the Amino-acid biosynthesis pathway.3. The pyruvate binding site should be stable and able to withstand changes in environmental conditions.4. The designed protein must have a Schiff-base intermediate with substrate that is structurally stable and resistant to denaturation.5. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "MSILSGR",
    "response#2": "MLTITFAVIASIIIAIIYSKSQKLVP",
    "response#3": "MIVVTR"
  },
  {
    "reference": "MTIKIGINGFGRIGRMVFRAAIANFKDIEVVAINDLLEPDYLAYMLKYDSVHGRFDGEVSVDGNTLVVNGKKIRLTAVKDPAELKWGEVGADVVVESTGIFLTKEGAQKHIDAGAKKVIMSAPSKDDTPMFVYGVNHGTYKGEAIISNASCTTNCLAPVAKVLNDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGVVIPQLNKKLTGMSFRVPTSDVSVVDLTVELEKAASYDEICAEMKAQSQGALKGVLGYTEDKVVATDFRGDARTSIFDAEAGIALDGTFIKVVSWYDNEWGYSNKVLEMARVVAK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that can perform NAD binding, NADP binding, glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity in cytoplasm to support glucose metabolic process, glycolytic process.2. For general function, the protein need meet that The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH; Catalyzes the oxidative phosphorylation of glyceraldehyde 3- phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD; The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.3. The designed protein should have a high specificity for D-glyceraldehyde 3-phosphate, NAD(+) binding, with minimal off-target effects.4. Ideal protein should have a binding affinity for key enzymes in the pyruvate from D- glyceraldehyde 3-phosphate: step 1/5, Carbohydrate degradation pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MKVVINGFGRIGRLVLRQILKRNSSVEVLAINDLVPGDALTYLFKFDSTHGRFPEDVRCEADHLIVGKRKIQFLSERNVQNLPWKDLGVDLVIECTGLFTKKEDAEKHIQAGAKRVLISAPGKGDIPTFVMGVNHKTFNPEKDFVISNASCTTNCLAPLSKVIDDNFGIIEGLMTTVHASTATQKIVDGASDKDWRGGRGALQNIIPSSTGAAIAVGKVLPNLNGKLTGMAFRVPVSNVSVVDLTVRYKKSATYSEICQKIQQASQKEMKGIMGYTEDEVVSTDFNGEELTSIFDAKAGLSLNKNFVKLIAWYDNEYGYSNRVVDLIAHMAKND",
    "response#2": "MKVVINGFGRIGRLVLRQILKRNSSVEVLAINDLVPGDALTYLFKFDSTHGRFPEDVRCEADHLIVGKRKIQFLSERNVQNLPWKDLGVDLVIECTGLFTKKEDAEKHIQAGAKRVLISAPGKGDIPTFVMGVNHKTFNPEKDFVISNASCTTNCLAPIAKVLLDNFGITEGLMTTVHAATATQLVVDGPSKKDWRGGRGCLQNIIPASTGAAKAVTLCLPELKGKLTGMAFRVPIEDVSVVDLTVRLDKSTTYDDICKAMKQASETDLKGILDYTDEQVVSSDFIGSEYSSIFDALAGIALNDRFFKLVAWYDNEWGYSCRLVDLIVHMAKQD",
    "response#3": "MKVVINGFGRIGRLVLRQILKRNSSVEVLAINDLVPGDALTYLFKFDSTHGRFPEDVRCEADHLIVGKRKIQFLSERNVQNLPWKDLGVDLVIECTGLFTKKEDAEKHIQAGAKRVLISAPGKGDIPTFVMGVNHKTFNPEKDFVISNASCTTNCLAPIAKVLLDNFGITEGLMTTVHAATATQLVVDGPSKKDWRGGRGCLQNIIPASTGAAKAVTLCLPELKGKLTGMAFRVPIEDVSVVDLTVRLDKSTTYDDICKAMKQASETDLKGILDYTDEQVVSSDFIGSEYSSIFDALAGIALNDRFFKLVAWYDNEYGYSTRVVDLLAHVAGKAK"
  },
  {
    "reference": "MAAGLGTRMKSKRAKVLHELGGLPLIGHVCRTAKALDPKTIFVIVGHQADEVAKAVESEVGDLATCVLQKEQRGTGDAVAAARKQLENSPSLLLVLSGDVPLIRAETLRKLVDEHRNSRNDCTILSVRLENPTGYGRIIRDGTGAFQRIVEHKDATPEERHVREINSGIYCFDTRQLFAALKKVEPANQQGEYYLTDVAEILLRDGNRVGVYLHSDAREVSGINTRAELAEFENLLRRNTIRSLMIEGGVTFIDPSHAYISAEAQLGRDCIIYPDVSIEGKSVLGEGCVIRSGARITSSHLGNNVTVNDHSLIVDSEIESNCSVGPFAHLRMNARLEEKATVGNFVEVKKSRIGRGTKAMHLSYLGDATIGDDTNIGAGTITCNYDGKHKHETFIEENVKIGSDTMLVAPVRVGARSVTGAGSVVTKDVPPDSLVAGVPATVKKTLKK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein contains novel MobA-like NTP transferase domains that confer a unique function or activity.2. The designed protein should have a high specificity for acetyl-CoA binding, with minimal off-target effects.3. The protein design should take into account the physiological conditions of the Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway.4. The presence of Mg(2+) is necessary for the protein to function in the desired environment.5. The protein design should aim to mimic or enhance the function of natural proteins involved in cell morphogenesis, regulation of cell shape, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "VVIMAAGLGTRMKSATPKVLHEIGGRSLVGHAVVTARALDPEHLVVVVGNGRELVEAHLKQIDPEAKAAVQEQQLGTGDAVRAGLSAVPEDFEGSVVVTSGDVPLLEAETLQELVAEHDKQGNAVTVLTARVADPTGYGRIIPGEDGSVAGIVEHKDATPEQRQIDEINAGIYVFDAATLRDGLSRITTDNAQGELYLTDVLAIARDDGKRVGAFVTEDYQETMGINDRVALAQAESVMRARINEKHLRNGVILKDSSHFILRGTLKHGKNVEIDTSVIIENHVILGDDVKIGPGCIIKNSSINSKTHIQAYTIIENCKIGKNCIIGPFSHIKSNTILDKHVQIGNFVEIKNSIIQKKSKVKHLSYIGDAELGSKVNFGAGSITCNYDGVNKFKTIIGNNVFIGANTQLIAPIKIIKNTTIAAGTTVKKDVNVKCLVYDKKEQRNIEGWVQKKRPGTAAAEAAEAAQNKED",
    "response#2": "VVIMAAGLGTRMKSATPKVLHEIGGRSLVGHAVVTARALDPEHLVVVVGNGRELVEAHLKQIDPEAKAAVQEQQLGTGDAVRAGLSAVPEDFEGSVVVTSGDVPLLEAETLQELVAEHDKQGNAVTVLTARVADPTGYGRIIPGEDGSVAGIVEHKDATPEQRQIDEINAGIYVFDAATLRDGLSRITTDNAQGELYLTDVLAIARDDGKRVGAFVTEDFNETLGVNDRVALADAERIMRRRINEGHMRNGAIMIDPSSTYIDSDVKIGKDTVIYPGVIIEGNTEIGEECVIGHNSRIVDSKIGDRVNIQSSTIIESEVDNDTNIGPYAYLRPNSKIGKSVKIGDFVEVKNSTIGDKSKASHLAYIGDAEVGKNVNIGCGVVFVNYDGKVKHKTIVEDNAFVGSNSNLVAPVIVRENGYVAAGSTITEEVKSGALSIARARQVNKEGWAEKKRAAG",
    "response#3": "VVIMAAGLGTRMKSATPKVLHEIGGRSLVGHAVVTARALDPEHLVVVVGNGRELVEAHLKQIDPEAKAAVQEQQLGTGDAVRAGLSAVPEDFEGSVVVTSGDVPLLEAETLQELVAEHDKQGNAVTVLTARVADPTGYGRIIPGEDGSVAGIVEHKDATPEQRQIDEINAGIYVFDAATLRDGLSRITTDNAQGELYLTDVLAIARDDGKRVGAFVTEDDAEETLGINDRIQLAEAEKVMRERVNRGHMLNEAMEQGCTLQDPDTVWFSHDTELGQDVSIGPNVVLGPGVKVGDNVQIKPFXHIEGATIENXAVXGPFARXRPGAEIGRDVXVGNFVEIKQSQLERYVXVNHLSYXGDSVLGEGVNIGAGTITCNYDGFNKSLTEIGPKAFIGSNNSLVAPVKIGAGAITAAGSVITQDVEEDALAITRSSRETIPGWGVMLSDPERFDLRGELQHGKDVFIDANVILEGQVTLGNRVRIGTGCVLKNAIISDDVVISPYTMIEDGRVAAHSTLGPFARLRPGSELGEGAHVGNFVEIKQARLGKGSKAGHLSYLGDAEVGAHVNIGAGTITCNYDGANKHKTVIGDDVFIGSYSQLIAPITIGHGSTIGAGTTVTHDVADGEMVISRIRGVPAKELKRG"
  },
  {
    "reference": "MNEIVVFSGNSNRTLAEAVCRYLDMPLAAAKVGSFSDGEIQIEIDENVRKKDVFVIQSTCSPVNDHLMELLLMIDAFRRSSASRITAVMPYYGYARQDKKVAPRVPISAKIVADLLETAGARRMITMDLHAGQIQGFFNIPVDNLFAAPVLISHIKENFDPDAVIVSPDAGGVERARAFAKRLGAGLAIVDKRREAPNEAKAMAVIGDVAGKIAIILDDMIDTGGTLTEAAGALLKKGAREVHACCSHPVLSGKAVERITNSGISSVLVTDTIPLQKKARECGKIDALSIAELVGEAIVRSHKGDSVTSLFV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must possess ribose phosphate diphosphokinase activity to support ribonucleoside monophosphate biosynthetic process under certain conditions.2. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.3. A protein should be able to manipulate the activity of enzymes in the 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "MPLLASYFVDSKLENITVVSPDHGGVTRARSFAKMLGTPIAIIDKRRPEANKAEVMNIIGDVAGRTCIMVDDIIDTGGTLMAGANALLAN",
    "response#2": "MPLLASYFVDSKLENITVVSPDHGGVTRARSFAKMLGTPIAIIDKRRPEANKAEVMNIIGDVAGRTCIMVDDIIDTGGTLMAGANALLG",
    "response#3": "MPLLASYFVDSKLENITVVSPDHGGVTRARSFAKMLGTPIAIIDKRRPEANKAEVMNIIGDVAGRTCIMVDDIIDTGGTLMAGANALLG"
  },
  {
    "reference": "MQCHRDLALSQALWGWQLSKQSGWAHPSLPHSPLPSTVHSCSWAPPHLQRHLPLATVSPGTTQLTQGPAGRTLGQTQASCPEPRPSMDAVDATMEKLRAQCLSRGASGIQGLARFFRQLDRDGSRSLDADEFRQGLAKLGLVLDQAEAEGVCRKWDRNGSGTLDLEEFLRALRPPMSQAREAVIAAAFAKLDRSGDGVVTVDDLRGVYSGRAHPKVRSGEWTEDEVLRRFLDNFDSSEKDGQVTLAEFQDYYSGVSASMNTDEEFVAMMTSAWQL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should contain one or more EF-hand domains that are essential for its function.2. The protein should exhibit Polar residues compositional bias.3. The protein must exhibit the following characteristics: Calcium-binding protein; May play a role in cellular signaling events (Potential)..4. helix-turn-helix-strand-helix-turn-helix-strand-helix-helix-strand-helix-helix-helix-helix is the desired arrangement for the engineered protein.5. The ideal protein would be one that plays a critical role in intracellular signal transduction through calcium ion binding within vesicle, cytoplasm.6. The Ca(2+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "LAEKFRQKASLHEAWTRGKEEMLSQRDYDSALLQEVRALLRRHEAFESDLAAHQDRVEHIAALAQELNELDYHEAASVNSRCQAICDQWDNLGTLTQKRRDALERMEKLLETIDRLQLEFARRAAPFNNWLDGAVEDLQDVWLVHSVEETQSLLTAHDQFKATLPEADRERGAIMGIQGEIQKICQTYGLRPCSTNPYITLSPQDINTKWDMVRKLVPSCDQTLQEEG",
    "response#2": "LAEKFRQKASLHEAWTRGKEEMLSQRDYDSALLQEVRALLRRHEAFESDLAAHQDRVEHIAALAQELNELDYHEAASVNSRCQAICDQWDNLGTLTQKRRDALERMEKLLETIDRLQLEFARRAAPFNNWLDGAVEDLQDVWLVHSVEETQSLLTAHDQFKATLPEADRERGAIMGIQGEIQKICQTYGLRPCSTNPYITLSPQDINTKWDMVRKLVPSCDQTLQEED",
    "response#3": "LAEKFRQKASLHEAWTRGKEEMLSQRDYDSALLQEVRALLRRHEAFESDLAAHQDRVEHIAALAQELNELDYHEAASVNSRCQAICDQWDNLGTLTQKRRDALERMEKLLETIDRLQLEFARRAAPFNNWLDGAVEDLQDVWLVHSVEETQSLLTAHDQFKATLPEADRERGAIMGIQGEIQKICQTYGLRPCSTNPYITLSPQDINTKWDMVRKLVPSCDQTLQEES"
  },
  {
    "reference": "MTTMPDMQKTSDTRKIPINKVGVKDISYPIVVMDKNKKFQQTVARINMYVDLPHHFKGTHMSRFVEILNAYREDIALDKMEPILQKMKEKLGASSAHLEIEFPYFIEKRAPVSGARSLMEYTCTFCGSLGEEFDFVLGVQIPVTSLCPCSKELSRYGAHNQRSHITVRLRYTDFIWIEDLVAMIEECGSSPVWSLLKRVDEQYVTERAYENPKFVEDIVREVTQKLLAHDAITWFSVEAENFESIHKHSAYAAIERDKKMT",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be optimized for activity in 7,8-dihydroneopterin triphosphate from GTP: step 1/1, Cofactor biosynthesis, 7,8-dihydroneopterin triphosphate biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The protein should be designed to catalyze the following reaction GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate +   H(+).3. For general function, the protein need meet that Converts GTP to 7,8-dihydroneopterin triphosphate. The designed protein sequence is ",
    "response#1": "EIAVRMILEAIGEDPDREGLKDTPKRVARMYEEVFAGLSQDPSEHLERYFTEEHEEMVLVKDIPLYSMCEHHLLPFYGKAHVAYIPRKGKVTGLSKLARVVEGFAKRPQLQERLTSQIADAIMEKLNPRGVLVVIEAEHMCMTMRGVKKPGSKTITSAVRGIFATSVATRAEAMALF",
    "response#2": "EIAVRMILEAIGEDPDREGLKDTPKRVARMYEEVFAGLSQDPSEHLERYFTEEHEEMVLVKDIPLYSMCEHHLLPFYGKAHVAYIPRKGKVTGLSKLARVVEGFAKRPQLQERLTSQIADAIMEKLNPRGVLVVIEAEHMCMTMRGVKKPGSKTITSAVRGIFATSVATRAEAMALLA",
    "response#3": "EIAVRMILEAIGEDPDREGLKDTPKRVARMYEEVFAGLSQDPSEHLERYFTEEHEEMVLVKDIPLYSMCEHHLLPFYGKAHVAYIPRKGKVTGLSKLARVVEGFAKRPQLQERLTSQIADAIMEKLNPRGVLVVIEAEHMCMTMRGVKKPGSKTITSAVRGIFATSVATRAEAMALLK"
  },
  {
    "reference": "MAIPGRGDVPRDPEEVLNDAKDFLGQYFASIRRANTPAHEARWKIVQEEVATTGTYQLTTTELVFGAKLAWRNASRCIGRIQWSKLQVFDCRQVTTTSGMFEALCNHIKYSTNKGNIRSAITLFPQRTDGKHDYRIWNSQLISYAGYRQPDGTVLGDPMHCEFTDLCLKLGWKPPRTAWDILPLVLSANGKDPDYFEIPRELVMEIHMTHPTFEWFKELELRWYALPAVSSMRFDCGGIEFTANAFNGWYMSTEIGCRNFCDTNRLNVLEKIAQNMGLDTRTPVNLWKDKALVEVNVAVLHSFQQHNATIVDHHTASESFIKHLDNENRLRSGCPADWIWIVPPMSSSITPVFHQEMALYYLKPSYEYQEPAWKTHQWQKSKPITGKRPINRKFHFKQIARAVKFTSKLFGRALSKRIKATVLYATETGKSEQYAKELGVIFGHAFNAQVHCMADYDITSIEHEALLLVVTSTFGNGDPPENGVAFGEHLCEILYADQVGEDSTGNQMLTPKSFIKANSDIQRYTAGNPKKLNRLESLKGSTTDATSIDSFGPLSNVRFAVFALGSSAYPNFCNFGKYVDKLLVDLGGERIHDLATGDEMCGQDQAFRKWASSVFNVACETFCLDDDETLQEAKRALGTVALSEETVQFARAGCRPATLHAALQKSLNKQFVSCTVKANKDLGDASAERSTIFIDLEPKEEIKYNPGDHVGIIACNRKELVESLLSRIKDVDDYDEPLQLQLLKETHTSSGLVKSWEPHEKLPIMSVR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a fast turnover rate for H(+) + 2 L-arginine + 3 NADPH + 4 O2 = 4 H2O + 2 L-citrulline   + 3 NADP(+) + 2 nitric oxide.2. The designed protein should have a high specificity for heme b, L-arginine, (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin, FAD, FMN binding, with minimal off-target effects.3. For general function, the protein need meet that Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body.4. The designed protein have Flavodoxin-like, FAD-binding FR-type domains with specific sequence motifs that are crucial for its activity.5. The protein design should incorporate a binding pocket that specifically recognizes FMN to enhance enzymatic efficiency.6. A protein with nitric-oxide synthase activity, flavin adenine dinucleotide binding, calmodulin binding, FMN binding functions is required to enable nitric oxide biosynthetic process. The designed protein sequence is ",
    "response#1": "MLSQNTIKVIKSTVPVLEVHGEAITRHFYETMFEHHTELLNIFNHANQKQGRQQAALANMVYTAAQHIDNLGSILPAVRQVAHKHRSLGIVPEQYAIVGTYLLQAIKDVLGDAATDEIITAWSEAYNVIADAFIGVESEMYAESAESGGWRGTREFRITEKQPQSSLITSFVLEPVDGLPVADFQPGQYLAVYIRDASLENQEIRQYSLTQAPNGKTYRIAVKREDQGAVSNFLHNRAQQGDIVHLAAPHGDFFLDASAARPVVLISGGVGLTPLTSMLETVAADRPARQVTFVHAAQHGGLHAMREQVAELAARHPQVSVHVCYAQPMPGDQDDAYHPDGYIDLSWLAQQVPSDAHFYLCGPVPFIDLVARQLRDAGVPDDRIHYEVFGPHAEL",
    "response#2": "MLDAQTIATVKSTIPLLAATGPKLTAHFYDRMFAHNPELKDIFNMSNQRNGDQRQALFDAICAYATNIENLAALLPAVERIAQKHTSFTIKPEQYDIVGGHLIATLDEMFSPGQEVLDAWGKAYGVLANVFIHEADQPLLTPVADFRSFRVTNKVKESEVITSFYLKPIDGGALPSFEPGQYVSVKVQPIGEEYTHIRQYSLSEAPGKDYFRLSVRREDAKDDQPQGIVSTYLHNHIEEGDQLQLSAPAGDFFLQFESDRPVVLLSGGVGLTPMISMLNTLTQKDYKPFAISRKVKESDLITSIYLKPKDGKPTASFLSGQYISVRVNIPGDEHTHIRQYSLSDAPNGEYYRITIKRESEEKATLGAVSNYLHDQLKEGDSIEISAPAGEFVLAPLPESADVDFYFCGPVPFMRAVATALQAWGVAEEHIHYEFFGPAASLEAA",
    "response#3": "MLSQNTIKVIKSTVPVLEVHGEAITRHFYETMFEHHTELLNIFNHANQKQGRQQAALANMVYTAAQHIDNLGSILPAVRQVAHKHRSLGIVPEQYAIVGTYLLQAIKDVLGDAATDEIITAWSEAYNVIADAFIGVEEQLFRDNGWREFVVERKERESQLITSFYLRPADGGPLPPFVPGQFVSLVLPIPELGHEQIRQYSLSEAPNTAYFRISVKREGQDPQGAVSNRLHDAVNVGDRIRLSPPYGDFYLQRKGDTPVAVLISGGVGLTPLTSMLETVATTHPTRPVTFIHAAQQAELHVMREQVRQLAADNARLSSYVVYHAPTASDRQQHGHFDIDGLLDLSVLPDDRAHFYLCGPVPFMNMVYHQLVKLGVPADRMRFEFFGPAVSL"
  },
  {
    "reference": "MASEIHMPGPVCLTENTKGHLVVNSEALEILSAITQPVVVVAIVGLYRTGKSYLMNKLAGKNKGFPLGCTVKSETKGIWMWCVPHPSKPNHTLILLDTEGLGDMEKSDPKSDSWIFALAVLLSSSFVYNSMGTINHQALEQLHYVTELTELIRAKSCPRPDEVEDSSEFVSFFPDFIWTVRDFTLELKLDGHPITEDEYLENALKLISGKNPQIQNSNKPREWIRHFFPKQKCFVFDRPINDKKLLLHVEEVREDQLDSNFQMQSENFCSYIFTHAKTKTLREGILVTGNRLGMLVETYLDAINSGATPCLENAMAVLAQCENSAAVQRAANHYSQQMAQQVRFPTDTLQELLDVHAVCEREAIAVFMEYSFKDKSQEFQKKLVDTMEKKKEDFVLQNEEASAKYCQAELKRLSELLTESISRGTFFVPGGHNIYLEAKKKIEQDYTLVPRKGVKADEVLQSFLQSQVVIEESILQSDKALTAGEKAIAAKQAKKEAAEKEQELLRQKQKEQQQMMEAQERSFQENIAQLKKKMERERENYMRELRKMLSHKMKVLEELLTEGFKEIFESLNEEINRLKEQIEAAENEEPSVFSQILDVAGSIFIAALPGAAKLVDLGMKILSSLCNRLRNPGKKIIS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. We are looking for a protein with broad substrate specificity for GTP + H2O = GDP + H(+) + phosphate.2. The designed protein should be involved in regulation of I-kappaB kinase/NF-kappaB signaling, negative regulation of cytokine production by localizing to membrane and exhibiting GTPase activity.3. For general function, the protein need meet that May help to assemble NADPH oxidase on phagosomal membranes by acting as a bridging protein between NADPH oxidase cytosolic subunits NCF2-NCF4 and the membrane subunits CYBA-CYBB  The designed protein sequence is ",
    "response#1": "EGIVVNGNRLATLVTTYVDAINSGDVPCLENAVTTLAQRENSIAVQKAADHYSEQMAQRMRLPTDTLQELLTVHTACEKEAIAVFMEHSFKDENQQFQKNLVVTIEEKKEDFLRQNEAASLSHCQAELDKLSESLRESISRGVFSVPGGHRLYLEARKKVEQDYERVPRKGVKANHVLQSFLQSQISIEDSIMQSDKALTDGQKAMEAERAQKEAAEKEQELLRQKQKELQQVMEAQERSYKENVAQLHEKMETERKNILREQEVKLEHKLKIQKDMLNEGFKRKCEAMDLEISQLL",
    "response#2": "EGIVVNGNRLATLVTTYVDAINSGDVPCLENAVTTLAQRENSIAVQKAADHYSEQMAQRMRLPTDTLQELLTVHTACEKEAIAVFMEHSFKDENQQFQKNLVVTIEEKKEDFLRQNEAASLSHCQAELDKLSESLRESISRGVFSVPGGHRLYLEARKKVEQDYERVPRKGVKANHVLQSFLQSQISIEDSIMQSDKALTDGQKAMEAERAQKEAAEKEQELLRQKQKELQQVMEAQERSYKENVAQLHEKMETERKNILREQEVKLEHKLKIQKDMLNEGFKRKCEAMDLEISQLL",
    "response#3": "EGIVVNGNRLATLVTTYVDAINSGDVPCLENAVTTLAQRENSIAVQKAADHYSEQMAQRMRLPTDTLQELLTVHTACEKEAIAVFMEHSFKDENQQFQKNLVVTIEEKKEDFLRQNEAASLSHCQAELDKLSESLRESISRGVFSVPGGHRLYLEARKKVEQDYERVPRKGVKANHVLQSFLQSQISIEDSIMQSDKALTDGQKAMEAERAQKEAAEKEQELLRQKQKELQQVMEAQERSYKENVAQLHEKMETERKNILREQEVKLEHKLKIQKDMLNEGFKRKCEAMDLEISQLV"
  },
  {
    "reference": "MADENYTRITEFIFIGLRYHPNLQVFLFLLFLLFYLVTMTGNLGMIILIRVDSRLHTPMYFFLSHLSFVDICFSSVVAPKMLTDFFADKKAISFLGCVLQQWFFGFFVAIECLLLASMAYDRYVAICNPLLYSVAMSQRLCIQLVIGPYAVGFFNTMTHTTAAFRLPFCGSNIINHFFCDMSPILSLICADIRINKLLVFIVAGAVLIVSSTTIIVSYFHILIAILRIRSAEGRRKAFSTCSSHVTAVSILYGTLFFIYVRPSAISSLDLNKVVSVFYTAVIPMLNPLIYSLRNKEVKSAMGRTVAKAKVFLKN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein localizes to the membrane, plasma membrane. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWKEATTTLFCASNAKAYDTEVHNVWATHACVPTDPSPQEVVLKNVTENFNMWKNNMVEQMHEDVISLWDQSLKPCVRLTPFCVTLNCTDWKNANNTQNMTNTNNNTNGGMMEGEIKNCSFKVTTSIGNKRKKEYALFYNLDVVPIADDKTSYMLKHCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGIGPCRNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVIRSENFTNNAKIIIVQLNESVVINCTRPSNNTRKSIHMGWGRAFYATGKIIGDIRRAYCNISREKWNNTLRMIAEKLKGQFNTTIIEFNQSSGGDPEIVMHSFNCGGEFFYCNTTKLFNNTWNNSAWMHNEGSNETEITILCRIKQIINRWQDIGKAMYAPPIQGQIRCSSNITGLLLTRDGGNNRNESSETFRPGGGDMRDNWRSKLYKYKVVKIEPLGVAPSKARRRVVQREKR",
    "response#2": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQFLGLWGCSGKIICTTAVPWNTTWSNKSYDEIWDSMTWIEXEREISNYTTQIYEILTESQDQQDRNEKELLALDQWASLWNWFNITQWLWYIKIFIMIVGGLIGLRIIFAVLSIVKRVRQGYSPLSFQTPD",
    "response#3": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQFLGLWGCSGKIICTTAVPWNTTWSNKSYDEIWDSMTWIEXEREISNYTTQIYEILTESQDQQDRNEKELLALDQWASLWNWFNITQWLWYIKIFIMIVGGLIGLRIIFAVLSIVKRVRQGYSPLSFQTPD"
  },
  {
    "reference": "MTATHKPNLAGLSRAKLRALFVDMGEKPFRAEQILKWIHQRGVLDIDVMTDISKDLREKLKQTTEISLPKVLEDFKSEDGTRKWIVEVASGSHVEMVFIPEAGRGTLCISSQAGCSLDCSFCATGKQGFNSNLTVAEIAGQLWWANYHLGIFEDKAEQRVSNVVMMGMGEPLLNFENVMEALSIMMEDCAYGLSKRKVTISTSGVVPAIDRLKDYTDASLAISLHAPNDELRSQIMPINNKYPIKEVLRSVQGYMDSLSDKRVPVIEYTMIAGVNDHRQHARDLAILLEQFPCKINIIPFNPFSLSDYQRPSSSSVTNFRQILQKAGYTVTVRTTRGDDIGAACGQLVGDVEDQTRRSERHKRRHEERELARKEDSKVFGSTDNLAVAADRL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should contain one or more Radical SAM core domains that are essential for its function.2. The designed protein should have a binding site that is specific to the target [4Fe-4S] cluster, S-adenosyl-L-methionine ligand.3. The design of the protein must focus on the precise arrangement of amino acids in the S-methylcysteine intermediate, Proton acceptor to enable effective catalysis of adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.4. A protein that can perform rRNA (adenine-C2-)-methyltransferase activity, metal ion binding, rRNA binding, 4 iron, 4 sulfur cluster binding, tRNA (adenine-C2-)-methyltransferase activity in cytoplasm to support rRNA base methylation. The designed protein sequence is ",
    "response#1": "MKKPPPHLADLDPAGRKALLEDNGLPGFRAKQLSTHYFARLVDAPEQMTDLPAAQRGELVSTLLPELMTPVRTSEADRGTTRKTLWRLFDGALVESVLMRYTDRTTMCVSSQAGCGMACPFCATGQGGLQRNMSTAEIVEQVMAGARSLARGEVPGGPGRVNNVVFMGMGEPLANYKAVVGAVRRLTDPAPDGLGMSARGVTVSTVGLVPRIRQLTEEGVPLTLALSLHAPDDELRNELVPINTRFSVAETVDAAWQYARTTKRRVSIEYAMMRGINDQGWRADLLADVLYATGADWGWVHVNLIPLNPTPGSKWTASDPADEQEFVRRLEARGIPTTVRDTRGREIDGACGQLAASEAGETA",
    "response#2": "SSQVGCALDCSFCATARQGFNRNLENWEIVAQMWVANKALGCLPKEERVISNVVFMGMGEPLLNVTHTFPAARILMDDNAYGLSKRRVTISTAGVVPAIDKIKAALDVSLAISLHAPNNALRDELVPINQKYPLEVLMPALHRFVEGGHSKKHVTVEYVMLDHVNDRCPFTDVPSDKRPLIHGLSKSEIGQIVASLGQPSYRATQIWEWLYKRMAFEWNAMSNLPLALREHLDRELAICPGTLTARSADASGSEKLLLTLRDGECVEQVLIPAPGRKTVCVSSQVGCRFKCAFCASGQAGFRRNLEAGEIVAQTLWAALIWERRPSNVVFMGIGEPLDNYDNVLKAIRIINDSEGLGIGARKITISTSGIIPGMERLAGEGLQIELSVSLHAPTQELRSRLMPVARLYPLDRLIRACRSYFEKTKRLITFEYVLIRGTNDQASHARKLAELLSDFPCRVNLLLLSPVPEFKGTSSSPDAANAFIKTLSRAGINATLRAPRGLSVNAACGQLRSSG",
    "response#3": "YVYGYSICISTQIGCRMGCKFCASTIDGRVRDLTAGELIGEILAVQKDLGQRISNIVLMGSGEPLDNYENVIKFIKMVNEKYSLNIGQRHITLSTCGIVPKIYDLAREGLQITLAISLHAPNDIIRKQSMPVANSYSIDELIKSCQFYIDKTNRRVTFEYALIKGLNDSPSCAEELSKMLKGLLCHVNLIPINEVKEREYIKADKEIINEFKSILLKNGIEATLRREMGGR"
  },
  {
    "reference": "MRVTGTKRNYPLLWRWGIIIFWIMIIGNAQKLWVTVYYGVPVWKEAETTLFCASDAKAYETEKHNVWATHACVPTDPNPQEIHLENVTEYFNMWTNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLHCHNFTKTDNNTINDMPGEIKNCSFNATTELRDKKQKVYALFYRHDVVHMSEDNSSHYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCRDKKFSGKGTCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVIRSENITDNVKTIIVQLNETVTINCTRPNNNTRKSVRIGPGQTFYATGDITGNIRKAHCNVSSKQWSTTLQRVARQLRMYFKNKTIIFNDSSGGDVEITTHSFNCGGEFFYCNTTALFNSSWDANTLSNSTVNDTITLQCRIKQIVNMWQKVGQAMYAPPIPGIIKCESNITGLLLTRDGGNTDNITETFRPGGGNMRDNWRSELYKYKVVKIEPLGVAPTHAKRRVVGREKRAVGLGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLKLTVWGIKQLQARVLALERYLRDQQLLGIWGCSGKLICTTTVPWNSSWSNKTYNDIWNNMTWLQWDREISNYTSIIYGLIEESQNQQEKNEQDLLALDKWASLWSWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLNIVNRVRQGYSPLSFQTLTHHQREPGRPEGIEEGGGEQDKDRSVRLVSGFLALAWEDLRSLCLFLYHRLTDLVLIAART",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should emphasize Basic and acidic residues compositional bias.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The protein's functional design necessitates the inclusion of a YXXL motif; contains endocytosis signal, Di-leucine internalization motif motif.4. For general function, the protein need meet that Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes The designed protein sequence is ",
    "response#1": "MRVKEIRKNYQHLWKWGTMLLGMLMICSAEGEQLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVKLENVTENFNMWKNDMVEQMHEDVISLWDQSLKPCVKLTPLCVTLNCTDLTNITSATNGTISSGEGELKNCSFNITSSIRNKMKKEYALFYTLDIIPIDDDSNTTDSNTTYRLRSCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSKNFSDNAKTIIVQLNESVVINCTRPNNNTRKSITIGPGRAFYTTGQIIGDIRQAHCNLSRTQWNNTLKQIVGKLREQFGNNKTIVFNQPSGGDPEIEMHSFNCGGEFFYCNTTQLFNSTWIFNSTWNDTNTEGNDTITLQCRIKQFINMWQEVGKAMYAPPISGQIRCSSNITGLLLTRDGGINESRTNETFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRAVGIGALFLGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTAVPWNSSWSNKSQAEIWENMTWMQWDKEINNYTDIIYGLLEVSQNQQERNEKDLLALDKWKNLWNWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSLQTLLPAPRGPDRPEGTEEEGGERDRDRSNRL",
    "response#2": "MWVTVYYGVPVWKEAKTTLFCASDAKAQDQEVHNVWATHACVPTDPNPQEMILGNVTENFNMWKNDMVDQMHEDVISLWDQSLKPCVRLTPLCVTLNCTNVSINNSKSNVTNEEIGMKNCSFNITTEVRDKKKQVYALFYKLDVVQMDTNTSYRLINCNTSAITQACPKINFEPIPIHYCAPAGFAILKCNDKEFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIIIRSENITDNTKNIIVQLNEPVTIKCIRPYNNTRQGIHIGPGQALYTTDIIGDIRRAHCEINKTEWNTTLQQVAKKLGDLLNQTAIFQPSSGGDLEITTHSFNCGGEFFYCDTSELFNKTSNSTGGPRDTITLPCRIKQIINMWQGVGKAMYAPPIEGLIKCSSNITGLLLTRDGGTNNSKEETFRPGGGDMRDNWRSELYKYKVVRVEPLGLAPTRAKRRVVEREKRGLVLGFLFV",
    "response#3": "MRVMGIKKNWHLLWRWGMLFLGMLMICSAEDKKWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLENVTESFNMWKNNMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTHNVTATNDTATNGTISSGEGELKNCSFNITSSIRNKMKKEYALFYTLDIIPIDDDSNTTDSNTTYRLRSCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSKNFSDNAKTIIVQLNESVVINCTRPNNNTRKSITIGPGRAFYTTGQIIGDIRQAHCNLSRTQWNNTLKQIVGKLREQFGNNKTIVFNQPSGGDPEIEMHSFNCGGEFFYCNTTQLFNSTWIFNSTWNDTNTEGNDTITLQCRIKQFINMWQEVGKAMYAPPISGQIRCSSNITGLLLTRDGGINESRTNETFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRAVGIGAMIFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLRDQQLLGIWGCSGKLVCTTAVPWNSSWSNKSKTEIWDNMTWMQWDKEISNYTNTIYKLLEDSQIQQENNEKDLLALDKWNNLWSWFNITNWLWYIRIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLLFQTPTPNPGGPDRLGRI"
  },
  {
    "reference": "MAAVSGTRRTRAVSPTRWIVYAGSVFAGAWLATQLFYLVQIALWSFINPGSTAFMRTDAWWLSRDKPPAQVQHQWVPYDQISRNLKRALIASEDSTFATNNGYDVDAILQAWEKNKARGRIVAGGSTITQQLARNLFLSREKSYIRKGQELIITWMLETVLDKERIFEIYLNSVEWGRGVYGAEAAARYYYRIPASRLGAWQSARLAVMLPKPRWFDAHRGSAYQAQRAAVIARRMGAAELPQSQ",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should be able to enhance the efficiency of cell wall organization, regulation of cell shape. The designed protein sequence is ",
    "response#1": "AIGVLLTEDLTYAFEDMASVDFLAGVAQAAGDTQLTLIPASPASSVDHVSAQQLVNNAAVDGVVIYSVAKGDPHIDAIRARGLPAVIADQPAREEGMPFIAPNNRKAIAPAAQALIDAGHRKIGILSIRLDRANNDGEVTRERLENAQYQVQRDRVRGAMEVFIEAGIDPGTVPIMECWINNRQHNFEVAKELLETHPDLTAVLCTVDALAFGVLEYLKSVGKSAPADLSLTGFDGTHMALARDLTTVIQPNKLKGFKAGETLLKMIDKEYVEPEP",
    "response#2": "AIGVLLTEDLTYAFEDMASVDFLAGVAQAAGDTQLTLIPASPASSVDHVSAQQLVNNAAVDGVVIYSVAKGDPHIDAIRARGLPAVIADQPAREEGMPFIAPNNRKAIAPAAQALIDAGHRKIGILSIRLDRANNDGEVTRERLENAQYQVQRDRVRGAMEVFIEAGIDPGTVPIMECWINNRQHNFEVAKELLETHPDLTAVLCTVDALAFGVLEYLKSVGKSAPADLSLTGFDGTHMALARDLTTVIQPNKLKGFKAGETLLKMIDKEYVEPEP",
    "response#3": "AIGVLLTEDLTYAFEDMASVDFLAGVAQAAGDTQLTLIPASPASSVDHVSAQQLVNNAAVDGVVIYSVAKGDPHIDAIRARGLPAVIADQPAREEGMPFIAPNNRKAIAPAAQALIDAGHRKIGILSIRLDRANNDGEVTRERLENAQYQVQRDRVRGAMEVFIEAGIDPGTVPIMECWINNRQHNFEVAKELLETHPDLTAVLCTVDALAFGVLEYLKSVGKSAPADLSLTGFDGTHMALARDLTTVIQPNKLKGFKAGETLLKMIDKEYVEPEGS"
  },
  {
    "reference": "MKEVNVAVVGATGAVGNEMVKTLEQRAFPVKNLYPFASKRTAGQKINFHGEAIKVEELTEEAVKSKDIKIALFSAGASISKQFAPIFAHHGAVVVDNSSAFRQEPDIPLVVPEVNPHAIAQYKNRGIIANPNCSTIQMVVALKPIHDVARIKRVIVATYQAVSGTGKKAIDELERQVRELFNLQPVTCEVYPYQIAFNCLPHIDVFLENGYTKEEMKMFFETRKIMEDETITVSATCIRVPVFYSHSEAVHMQLEKPISPEEVKGLLAKAPGVKVVDDPSKNLYPLAIEAAGKDEVFVGRIRKDLAFENGIAMWVVSDNIRKGAALNAVQIAEVLLEKYL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should consider the endogenous proteins and enzymes present in the L-threonine from L-aspartate: step 2/5 pathway to avoid interference with native metabolic processes.2. The protein must exhibit NAD binding. The designed protein sequence is ",
    "response#1": "IKGAAGGIENTNYFVDTDQGQYVLTLFERLTFEQLPFYLHLMKHLATRGIPVPDPVADAKGNILHRLKGKPAAVVNKLRGHSELAPTPLHCAGVGEMLARLHLAGLDYERQQPNLRGLAWWNETVPVVLPYLTEEQRSLILGELAYQNHIAASSGYRSLPRGAIHADLFRDNVMFENGQLTGFFDFYFAGCDTFLYDIGICLNDWCIDLESGRLDTTRADAFMAAYQTVRPR",
    "response#2": "LKPLHDAAKIERINVATYQSVSGGGRSALEELGRQTGALLNFQDPDPQRFPVQIAFNLIPHIDDFLDNGYTKEEMKLVWETRKILGDDSIQVNPTAVRVPVFYGHSEAVNIETRDKLTAAQARTLLDAAPGVEVVDERGPGGYPTPVTHASGTDAVYVGRIREDLSHPRGLNLWIVSDNIRR",
    "response#3": "IKGAAGGIENTNYFVDTDQGQYVLTLFERLTFEQLPFYLHLMKHLATRGIPVPDPVADAKGNILHRLKGKPAAVVNKLRGHSELAPTPLHCAGVGEMLARLHLAGLDYERQQPNLRGLAWWNETVPVVLPYLTEEQRSLILGELAYQNHIAASSGYRSLPRGAIHADLFRDNVMFENGQLTGFFDFYFAGCDTFLYDIGICLNDWCIDLESGRLDTTRADAFMAAYQTVRPA"
  },
  {
    "reference": "MTNDNSGIWVLGYGSLIYKPPSHYTHRIPAIIHGFARRFWQSSTDHRGTPANPGRVATLIPYEDIIRQTAFLKNVNLYSESAPIQDPDDLVTIGVVYYIPPEHAQEVREYLNVREQNGYTLHEVEVHLETNREHEAELGEALEQLPRHNKSGKRVLLTSVYIGTIDNEAFVGPETVDETAKVIAVSHGPSGSNYEYLAKLEQALAQMPIMKERGRITDHYLTALLETVNKYR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein's architecture should predominantly feature strand-helix-strand-strand-strand-helix-helix-helix-strand-helix-turn-strand-helix-helix-turn.2. The protein should be optimized for stability and expression in cytoplasm where it will participate in glutathione catabolic process.3. The protein must exhibit the following characteristics: Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide; Acts specifically on glutathione, but not on other gamma-glutamyl peptides. The designed protein sequence is ",
    "response#1": "TPEERARVSMIEGAAVDLRQGLSRISYDPKFEQLKEGYLKDLPTTMKMWSDFLGKNPYLRGTSVSHVDFMVYEALDAIRYLEPHCLDHFPNLQQFMSRIEALPSIKAYMESNRFIKWPG",
    "response#2": "MGLELYLDLLSQPCRAVYIFAKKNDIPFELRIVDLIKGQHLSDAFAQVNPLKKVPALKDGDFTLTESVAILLYLTRKYKVPG",
    "response#3": "TVMFLVENEQRNILDQRFIEAELCKRNVRVIRRRLADVFERGTLDEERHLFIDGYEVAVAYFRTGYVPQDYTEQDWEARLMLERSRAVKCPDVPTQLF"
  },
  {
    "reference": "MSSIEQVNEVFDTILVLDFGSQYSHLITRRLREFNIYAEMLPCTQKIADLHFKPKGVIMSGGPYSVYAEDAPHVDHAIFDLGVPILGICYGMQELAWINGKQVARGEKREYGPATLNVLDKEDALFKNVDHSTVWMSHGDKLHGLPTGFKVIATSDNSPYCGIVHESKQIYGIQFHPEVTHSSNGKTLLKNFAVDLCHAKQNWTMKNFIGTEVQRIRDLVGPTAEVIGAVSGGVDSTVASKLMTEAIGDRFHAILVDNGVLRLNEAATVKKTLVDGLGINLTVVDAADEFLDNLKGVTDPEKKRKIIGNTFIHVFEREAEKIKPKDGKAIEFLLQGTLYPDVIESISFKGPSQTIKTHHNVGGLLENMKLKLIEPLRELFKDEVRELGELLGISHELVWRHPFPGPGIAIRVLGEVTREQVEIARKADHIYIEEIRKAGLYDKISQAFACLLPVKSVGVMGDQRTYEQVIALRAIETTDFMTADWYPFEHSFLRKVASRIVNEVDGVARVTYDITSKPPATVEWE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein can be targeted to cytoplasm and perform ATP binding, GMP synthase activity.2. The presence of Glutamine amidotransferase type-1 domains is critical for the interaction of the designed protein with its intended targets.3. The protein should have a high affinity for ATP.4. The For GATase activity of the protein must be engineered to enable effective catalysis of ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2   H(+) + L-glutamate.5. A protein that is capable of catalyzing reactions within the GMP from XMP (L-Gln route): step 1/1 pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "IVGDQIAQIRGQVGDRRVICGLSGGVDSAVAAALVQRAVGDQLTCVFVDHGLLRQGEAEQVKNDFVAATGADLVVADESQRFLDALAGVTDPETKRKIIGREFIRTFEDVAKRLNADQPIDFLVQGTLYPDVVESGGGEGAANIKSHHNVGGLPDDFQFSLVEPLRTLFKDEVRAVGLELGLPEDIVWRQPFPGPGLAIRIIGEVTAERLEVLRTADAITREEMGAAGLDRKVWQCPVVLLSDVHSVGVQGDGRTYGSPIVLRPVTSEDAMTADWARIPYDVLEKISTRITNACPQINRVVLDITSKPPATIEWH",
    "response#2": "NIITKLVKDLKNQIGSANVLLGLSGGVDSSVVAILLQQAVGKQLTCVFVDNGLLRLNEGNKIMQTFAQNMSVKVIRVNAQEKFYNALSNEDKPEAKRKIIGHAFIEVFEEEARKLNNIQFLAQGTIYPDVIESAGTKSNKTKVIKSHHNVGGLPNSLQLTLVEPIKELFKDEVRKIGVILGLPTHMLNCHPFPGPGLSIRILGQVKQEYVDILRQADTIFIDELYKNNLYNTVNQAFAVFLPIKSVGVTGDARRYDYVISLRAVETIDFMTARWARLPYDFLDLVSNRIMNEIPRISRVVYDISSKPPATIEWK",
    "response#3": "FREEAIEKIRAQVGTGKVICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGLLRKDEGKSVVDLFRHHYNIPLVHVDVSETFLGALKGVTDPEQKRKTIGKLFIDVFEAEARRVGGADFLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPERMNMKLVEPLRELFKDEVRALGRELGLPDVFVGRHPFPGPGLAIRCPGEITEEKLEILRNADAVYIDQIRKAGLYDVIWQAFAVLLPVRSVGVMGDGRTYDYVVGLRAVTSTDGMTADFYPFEMSFLGATATRIINEVKGVNRVVYDVTSKPPGTIEWITKLVKDLKNQIGSANVLLGLSGGVDSSVVAILLQQAVGKQLTCVFVDNGLLRLNEGNKIMQTFAQNMSVKVIRVNAQEKFYNALSNEDC"
  },
  {
    "reference": "MDKIPEELEKILQITREQNRWRRLETINLIPSENVMSPLAESVYMSDFMYRYAEGKPFKRYYQGTKYADEIEELAMKLVSEISSSKYADLRAVSGTIANAGVFRVLADSGDKAVIAPVQAGAHVSHTRFGTLGALGIEQIEMPYDQESMNVDVDKAIKLIEEVKPKFVTLGGSLYLFPHPVKELAPHVHAVGAKLVYDSAHVYGLIVGKAWHNPLEEGADVVTASTHKTFPGPQGGLIVTNDDSLYKKVSDTIFPWFVSNHHLHRLPSTAITALEMKYFGKEYAQQIVKNAKALAEALAAEGFKVIGEHLGFTKSHQVAIDVRNLGGGAKVAKLFEEANIIANKNLLPYDPPSAVKDPSGVRLGVQEMTRFGMKEEEMRIIARLMREVAIENKDVKEVKKKVTEFRKEFLEVKYTFTNVDLAKYSSKVISLLI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that contributes to tetrahydrofolate interconversion, glycine biosynthetic process from serine by functioning in cytoplasm with pyridoxal phosphate binding, glycine hydroxymethyltransferase activity.2. The protein must be engineered to have a high affinity for pyridoxal 5'-phosphate in order to increase its catalytic activity.3. A protein that is capable of catalyzing reactions within the glycine from L- serine: step 1/1, glycine biosynthesis, Amino-acid biosynthesis pathway with high efficiency and specificity.4. The protein must exhibit the following characteristics: Also exhibits a pteridine-independent aldolase activity toward beta- hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.; Catalyzes the reversible interconversion of serine and glycine with a modified folate serving as the one-carbon carrier. The designed protein sequence is ",
    "response#1": "LSDVDPDVAQAIQDELGRQRSTLEMIASENFAPRAVLEAQGSVLTNKYAEGYPGRRYYGGCEYVDVVENLARDRACELFGADHANVQPHSGAQANTAVMAALMQPGDTLMGLSLAHGGHLTHGMKINVSGKLYNIAAYEVEPETYRIDMDRVREQALEARPNVIVAGWSAYSRQLDFEAFRSIADEVGAKLWVDMAHFAGLVAAGLHPNPVPHAHVTTSTVHKTLAGPRSGVILCEADLKKKIDSAVFPGQQGGPLMHAIAGKAVAFKIAASEGFAERQRRTIEGAQILAERLTAPDLAEHGVSVLTGGTDVHLVLVDLRDSELDGKQAEDLLHRAGITVNRNAVPWDPRPPMVTSGLRIGTPALATRGFGAEQFTEVADVIADALDNPHDIDTITARVRELTSAFPLYPGV",
    "response#2": "ADYDPQLFAAIEDETRRQEEHIELIASENYCSPRVIEAQGTQLTNKYAEGYPGKRYYGGCEHVDIVEELAISRAKELFGATYANVQPHSGSQANAAVFMALLQGGDTVLGMSLAHGGHLTHGSHVSFSGKLYNAVQYGIDETTGQIDYAEVERLAVEHKPKMIIAGFSAYSGIIDWGKFREIADKVGAYLFVDMAHVAGLVAAGIYPNPMPHAHVVTTTTHKTLAGPRGGLILSSINDEDIYKKLNSAVFPGGQGGPLMHVIAAKAVAFKEALDPEFTTYQEQVVVNAKAMARTFIERGYNVVSGGTDNHLFLLDLISKDITGKDADAALGNANITVNKNSVPNDPRSPFVTSGLRIGSPAITRRGFGEEESVQLTHWICDILEGSDAAVEAKVKARVALTDKEIFNIIQLENNRQNNTIELIASENFASKSVMEAMGSQLTNKYAEGYPSKRYYGGCEEVDKIESLAIERLKKIFGCEHANVQPHSGSQANMAVYLSVLEPGDTIMGMNLSHGGHLTHGSPVNFSGRLFNFVAYGVNKETELINYDEVRSLALQHKPKMIVAGASAYSRVIDFKRLKQICDEVGAYFMVDMAHIAGLIAAGYHPSPVPYADFVTTTTHKTLRGPRGGAILCKEKYAKQVDKAIFPGIQGGPLMHVIAAKAVCFGEALKDDYKNYIEQVVKNAKVLEEELKKYDFKLVSGGTDNHLLLIDLTNKDITGKDAEKLLDSIGITVNKNTIPFETKSPFVTSGIRIGTPAVTTRGFKEEEMKEIAYLIKESLNKELKRQQSHIELIASENYVSKAVLELNGSVLTNKYAEGYPGKRYYGGCEFIDEIESLGIQTAKELFHAEHANIQPHSGSQANDAAYKALLEPKDRVVAMSLDAGGHLTHGYPINFSGYTYDFRFYGVNKDTEQLDYQEIEKIVLEHKPKLIVAGASAYSRIIDFKKFKEIADKVGAYLMVDMAHIAGLVAAGVHPNPLEYADIVTTTTHKTLRGARGGLILCKQEFAKKVDLAVFPGSQGGPLENLIAGKTQALLEASTDEFKEYGKQIVKNTKALANVLQENGLRLVAGGSDNHLINVDVKSTLRITGKKAEKILESIGIICNKNMIPFDTEKPFYTSGIRLGTPAMTTRGFKEEEFKQVGL",
    "response#3": "LDDVDPEIAPLLRDEARRQALGIELIASENFVSEAVLEAVGSVLTNKYAEGYPGRRYYGGCEVVDRVEQIAIDRAKALFGAGHANVQPHAGSQANEAVYRAAIEVGDTILAMNLDHGGHLTHGSPVNFSGKLYKVVPYGVRRDTEQIDYDEVRRLAQEHRPKLIQCGTTAYSRVLDFDAFRSIADEVGAVLFADIAHIAGLVAAGVHPSPIGKAHIVTTTTHKTLRGPRGGLILCDAAWAKKVDSAVFPGGQGGPLMHVIAGKAVAFLEASRPSFRDYARQIVANARALCDGMKRRGFRVVSDGTDVHLFMLSLVDRDVTGKQAQDVLERAGITTNKNMVPFDPRKPAVTSGVRVGTPAVTTRGMKEGEMTLLADWMAGFNGKEDLSDVDPDVAQAIQDELGRQRSTLEMIASENFAPRAVLEAQGSVLTNKYAEGYPGRRYYGGCEYVDVVENLARDRACELFGADHANVQPHSGAQANTAVMAALMQPGDTLMGLSLAHGGHLTHGMKINVSGKLYNIAAYEVEPETYRIDMDRVREQALEARPNVIVAGWSAYSRQLDFEAFRSIADEVGAKLWVDMAHFAGLVAAGLHPNPVPHAHVTTSTVHKTLAGPRSGVILCEADLKKKIDSAVFPGQQGGPLMHAIAGKAVAFKIAASEGFAERQRRTIEGAQILAERLTAPDLAEHGVSVLTGGTDVHLVLVDLRDSELDGKQAEDLLHRAGITVNRNAVPWDPRPPMVTSGLRIGTPALATRGFGAEQFTEVADVMADAIARPADLKVLADKQDPELWSAIEREEKRQQHNIELIASENIVSDSVRKAQGSVLTNKYAEGYPGKRYYGGCEFIDQVEQLAIDRAKAIFNAEYVNVQPHSGSQANAAAYAAFIKPGDKILGMDLNAGGHLTHGAKVSFSGTFYQSVSYGVDPQTEKLDYDEIRRIALKEQPQIIVAGASAYSRFIDWNKFREIADEVGAYLMVDMAHIAGLVAAGLHPSPVGIADVVTTTTHKTLRGPRGGMILSQEKYAKQLNFAVFPQNQGGPLEHVIAAKAVAFGEALQPEFKTYQKQVLKNAKAMAEVFENDTILHVVTGGTDNHLMTVDLTGTQLNGKEVQNLLDGVFITTNKEAIPEEKLSPFKTSGIRIGTPAITTRGFKEDDCREVANL"
  },
  {
    "reference": "MESSGNALARVVQEHAEPGDRVALVCGRGNNGGDAFVAARFLGDYDVEVVLLGHPDRIGTDIARENWVALQATEIPTTTITDSTDIDGLGVADVVVDAMVGTGVSGALREPAATAAAAINAAESTVVAVDVPSGVDADTGTADGVAVDADHVITFHDTKPGLEAIDATVTVADIGIPAAAERVVGPGDLLSRRRAADSHKGDHGELLVIGGGPYTGAPALAGQAALRAGADLVHVACPASVAPQVQGYSENLIVHPLAGEQVTPAVVDDLLARAAAVDTVVCGPGLGDSDTTREAVQLFLDAYDGRAVVDADALSVVPTVDTDATLVCTPHQGELVGMGGPNADDREQLADSVVEFAAELGHTLLVKGAHDIISDGETTRHNRTGNPGMTVGGTGDVLAGVTGALLATEEPLSAAAVGAYATGRAGDLAADIVGDSLVATDLLDRLPEAFVTDE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.2. The designed protein must possess NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity to support phosphorylation under certain conditions. The designed protein sequence is ",
    "response#1": "LIIGGSKEYSGAPALSALASLRTGADLVVILSPEPASIPQKSFSPNIISIPLKGEYFNESHVDIVKKYSERFDVIVIGPGMGLRDKTLYTLCRLIKELKNKKLVIDADALKALKDSKVTFEGNVVLTPHAGEFKIIAGLSSPLPSRTEERCRCVKEFSRKVNAVVLLKGHEDVISNGDKVKVNRTGNPGMTVGGTGDVLTGIVATFLAWSGDPFESACAAAFINGVAGDFAFSEKGYHLLATDVIEMIPR",
    "response#2": "VLMENAGLRVVMALRKRFPHPERDRAVVVAGKGNNGGDGFVVARHLFNSGGRPEVVLLARAGEVGGAAAVNLAVIREMGVTVTEAPDAAGWAKVRAKVARATVVVDAMFGTGLVKPLEGFYAAAVADINRSAAFKLSVDIPSGLSSDTFETIGPSVEADLTVTLAAPKR",
    "response#3": "LLLVGGDKGLGGAIRMAGEAALRSGAGLVRVLTHIEHVAPILAARPELMVQELTDETLEQGIHWADALVVGPGLGQSDWGRNALNLLQQSDKPALWDADALNLLAINPLKRQNWVLTPHPGEAARLLGCSVADIESDRLLSARNIVKQYGGVVVLKGAGTLIASEQGEMAIADVGNAGMASGGMGDILSGIIGSLIAQKLVLYDAACVGCVVHGVAADRLAEVQGTRGLLATDLLPVIA"
  },
  {
    "reference": "MAKQIFVTGGVVSSLGKGIIASSLGKLLQARGLRVTIQKFDPYLNVDPGTLNPYEHGECYVTNDGAETDLDLGHYERFLNMPTSQANNVTTGRIYLTVIENERRGDYLGKTVQVIPHITDEIKRRMLLLGNTGNYDVVITELGGTVGDIESLPYIEAVRQLRWELGNVNCMVIHLTLLPYLSAAKELKTKPTQHSVKELLSYGIQPDVIICRTEKHISPEIRRKIALFCNVESNAVIEAIDADTIYDVPLNLMKENLDAITLDKLRLSLYSKIDMSQWKEFLGKLKNSTSEVHIGLIGKYVELQDAYKSIQESFVHAGAANECRVIIESIHSEHLSPENVADKLQHLNGILVAPGFGQRGIEGKILAIQYARLHNIPFFGICLGMQCAVIEFARNVLKLNQADSTEMNANTPHPVISLMEQQKTITQKGGTMRLGAYQCELTPHTKATEIYGNKAIQERHRHRYEFNNAYLQEFENNGMKASGINPDTGLVEIMELPAHRWFIGTQFHPELQSTVLNPHPLFVDFIKNAILHKNSTS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates..2. The presence of Glutamine amidotransferase, CTP synthase N-terminal domains is critical for the interaction of the designed protein with its intended targets.3. The designed protein should be optimized for metal ion binding, CTP synthase activity, ATP binding, glutaminase activity activity in order to improve glutamine metabolic process, 'de novo' CTP biosynthetic process.4. The design of the protein must focus on the precise arrangement of amino acids in the active site, Nucleophile; for glutamine hydrolysis, Nucleophile to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).5. The protein design should take into account the physiological conditions of the Pyrimidine metabolism, CTP biosynthesis via de novo pathway, CTP from UDP: step 2/2 pathway.6. The designed protein should have a binding site that is specific to the target ATP, UTP, L-glutamine, Mg(2+), CTP ligand. The designed protein sequence is ",
    "response#1": "MQDAYKSIIESFIHAGAENDARVKLHWISAEDLETEKPETLLKGMYGILIPGGFGERGVEGKINAITFARENHIPFFGICLGMQCAVIEYARNVCGITKAQSSEFHKTRFNVIDIMPDQKNIKNIGGTMRLGAYPCIIEKNTKAYEAYKSEYITERHRHRYEVNNKFRAALQSEGLIFSGLSPNRELVEMIELPGHPWFVGCQFHPELKSRATKAHPLFRDFVKQIGGV",
    "response#2": "MQDAYKSIIESFIHAGAENDARVKLHWISAEDLETEKPETLLKGMYGILIPGGFGERGVEGKINAITFARENHIPFFGICLGMQCAVIEYARNVCGITKAQSSEFHKTRFNVIDIMPDQKNIKNIGGTMRLGAYPCIIEKNTKAYEAYKSEYITERHRHRYEVNNKFRAALQSEGLIFSGLSPNRELVEMIELPGHPWFVGCQFHPELKSRATKAHPLFRDFVRDADKSLCEALTHGGIANDTRVNIEWIEAESFEKEPHTVAERLKDKKAILVPGGYGERGTAGMISAIQYAREHQIPYMGICFGMQLAVIEVARHLAGIEKANTTEFGVTAEPVVGLLTEWLNGDVKERRSAEDDLGGTMRLGLYPAILAPGSLIQKIYGTSSIQERHRHRYELNIEYREKFEKAGLKFSGLSPDGLLPETVELEGHPWFVGVQFHPELRSRPFDPHPLFTSFIRDADKSLCEALTHGGIANDTRVNIEWIEAESFEKEPHTVAERLKDKKAILVPGGYGERGTAGMISAIQYAREHQIPYMGICFGMQLAVIEVARHLAGIEKANTTEFGVTAEPVVGLLTEWLNGDVKERRSAEDDLGGTMRLGLYPAILAPGSLIQKIYGTSSIQERHRHRYELNIEYREKFEKAGLKFSGLSPDGLLPETVELEGHPWFVGVQFHPELRSRPFDPHPLFTSFIE",
    "response#3": "MQDAYKSIIESFIHAGAENDARVKLHWISAEDLETEKPETLLKGMYGILIPGGFGERGVEGKINAITFARENHIPFFGICLGMQCAVIEYARNVCGITKAQSSEFHKTRFNVIDIMPDQKNIKNIGGTMRLGAYPCIIEKNTKAYEAYKSEYITERHRHRYEVNNKFRAALQSEGLIFSGLSPNRELVEMIELPGHPWFVGCQFHPELKSRATKAHPLFRDFVEQALGAVGSERDADKSLCEALTHGGIANDTRVNIEWIEAESFEKEPHTVAERLKDKKAILVPGGYGERGTAGMISAIQYAREHQIPYMGICFGMQLAVIEVARHLAGIEKANTTEFGVTAEPVVGLLTEWLNGDVKERRSAEDDLGGTMRLGLYPAILAPGSLIQKIYGTSSIQERHRHRYELNIEYREKFEKAGLKFSGLSPDGLLPETVELEGHPWFVGVQFHPELRSRPFDPHPLFTSFITESYKSLIEALNHAGMHTLSKVNIHYLDSEDIEKNGCGALKAMDAILVPGGFGRRGTEGKIAAIRYARENKVPYLGICLGMQLAVVEFARDVAGMNGAHSTEFDKHTDYPVIGLITEWLDASGKVETRGEDSDLGGSMRLGGQVCNLTDGTLARDIYGKPEITERHRHRYEVNNTLLAKLEEQGLRVAGRAPGTDLCEMIELPTEGPNAHPWFVGCQFHPEFTSNPRNGHPLFTAFVEAALGAVRDADKSLCEALTHGGIANDTRVNIEWIEAESFEKEPHTVAERLKDKKAILVPGGYGERGTAGMISAIQYAREHQIPYMGICFGMQLAVIEVARHLAGIEKANTTEFGVTAEPVVGLLTEWLNGDVKERRSAEDDLGGTMRLGLYPAILAPGSLIQKIYGTSSIQERHRHRYELNIEYREKFEKAGLKFSGLSPDGLLPETVELEGHPWFVGVQFHPELRSRPFDPHPLFTSFI"
  },
  {
    "reference": "MATTSTMQHTIAREVTLKGVGLHTGKEVTLKFIPAPEDHGYVFQRIDLPESPLVPADAQYVENTQRGTNLNKEGVHINTSEHVLSALVGLEIDNVLIQLDAPEPPIMDGSAKFFVEALESAGLVEQQKPRYEYVVKEVFTCKDEETGSEITLIPSDTYQVCTMVDFGTKILGTQNASLNDLSEFKSEIANARTFSFLHELETLLDHGLIKGGDLNNAIVYVDKEISHSTMEKLKKAFNKKELSVKPNGILDNLTLHYPNEAARHKLLDVIGDLALVGVRIRGKVIANKPGHYINTLFAKKLSKLIKEDKRKAVPQYDLNLPPLMDIHQIMDMLPHRPPFLFIDRILELSDSHVVGMKNVTMNEDFFVGHFPGAPVMPGVLQIEAMAQTGGILVLSTVPDPENYLTYFMKIDNVRFKHKVLPGDTLIFHCSLITPIRRGICHMQAYAYANGKMVCEAEMMAQIAKKN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The active site, Proton donor active site of the designed protein must be optimized for substrate binding and specificity.2. A protein that can modulate the overall flux of metabolites through the lipid IV(A) biosynthesis, Glycolipid biosynthesis, lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 2/6 pathway, enhancing or suppressing its activity as needed.3. The protein should have metal ion binding, 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity and be active in cytoplasm.4. The protein should have a high affinity for Zn(2+).5. The protein must exhibit the following characteristics: Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.; Involved in unsaturated fatty acids biosynthesis. The designed protein sequence is ",
    "response#1": "MDYKKSKLIDEINFKTFTGDLDTRVTEVTHDSRKALAGFLFVAIKGEKFDAHSFLADAIAKGAVAVVSEKPLPVPAGENVAVIQVKDTRRALALMAANYFNHPAKDMKIIGIT",
    "response#2": "MSVLSGRAYSPSRSRRLSTGARLALLLAVCAALGLWVGARADAAGGSTGAVAVVTEPGPVVRGAEVRGDLQVLREAGVPVRAVSAPAWETAEPAEIRERLRQAGVRVVRAVPDWLTVAGPGPDLQALRVDPAVAGVWLDGPTPDRLRAVAAAAGVPTVLSDRPVAELPGGRLLGGWRPGDDPQLLAAAGGVLDELRGSGYAVRALSLGPDWPTGTAADRAALERLAAAGVGLVGFVPQWTGPAGADPAAVGARLRAAPGVAWVALRPTPDGGGRAPRAAADLGSWLAALRADGLTAATLPGWLTPEVAGPADPAAAAAVAAAVAAGT",
    "response#3": "MLKGYTVEETLQLKQEGKLVAILPMMGDETIHYVAETSMAAMRSAHKAGADIVIAFHVDYTPNELVERIGQYGVMAGERHNYREQVIDGNKSLERYKQMGVDVIFAPELADIHPDSPYIKTNATITPGEIADLLCGADAINKAEYVIAEVEKAITLLEEAGIKYGIAHPECRPLIRLKERFADKVDFTTSGSMGSIAAALYIKEADRVVMLGFSGRYKSDVTGEKVKEIFQAVKEAEIISLDLLKLAVENMK"
  },
  {
    "reference": "MGGKWSKSSIVGWPQVRERIRRTPPGQTPPAAEGVGAVSQDLAKHGAVTSTNMNNADSVWLRAQEEDEEGVGFPVRPQVPLRPMTFKEAFDLSFFLKEKGGLDGLIHSKKRQEILDLWVYNTQGFFPDWQNYTPGPGTRFPLCFGWCFKLVPVDPREVEENTKGENNCLLHPMNQHGFEDEEGEVLMWKFDSALARRHIARELHPEYYKDC",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The ideal protein would be one that plays a critical role in suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host autophagy through GTP binding, SH3 domain binding within membrane, virion component, host cell Golgi membrane, host cell plasma membrane, extracellular region.2. For general function, the protein need meet that Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK13. The designed protein should contain at least one Diacidic; necessary for CD4 internalization, Dileucine internalization motif; necessary for CD4 internalization, PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "MGGKWSKNKSGGWSAVRDRMRQTEPAAEGVGAVSRDLEKHGAITSSNTAATNADCAWVEAQEEDGEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLVWSQKRQDILDLWVYNTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPLEPEQVEEANKGENNCLLHPISQHGMDDPEREVLQWKFDSRLAFHHMAREKHPEYYKDC",
    "response#2": "MGGKWSKSSIVGWPQVRERIRQTPTAGKGVGAVSQDLDKHGAITSSNINNADSTWLAAQEEENDVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPDKVEEATEGENNCLLHPMSQHGVEDSEREVLMWKFDSRLAFHHMAREKHPEYYKDC",
    "response#3": "MGSKWSKSWPAVRERMRRAEPAAEGVGAASQDLDKYGALTSSNTVSNNAACAWLEAQEEEEVGFPVRPQVPLRPMNYKAAVDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVEPEKVEEATEGENNSLLHPICQHGMDDEEKEVLMWKFDSRLAFHHVAREKHPEYYKDC"
  },
  {
    "reference": "MPPLPVLNRPQIHTSVTEISHAIDRTIKEELFPVAYTTEEEQYFKTNPKPAYIDELIKDAKEFIDLQYSLKRNKIVLITSGGTTVPLENNTVRFIDNFSAGTRGASSAEQFLANGYSVIFLHREFSLTPYNRSFSHSINTLFLDYIDSEGKIKPEFAENVLKNKKLYDKYMEKEEKLLLLPFTTVNQYLWSLKSIAKLLNNSGCLFYLAAAVSDFFVPYSRLPQHKIQSGDNGKMGANNDTEGTTRTTPDGKLIVNLDPVPKFLRRLVESWATQAMIVSFKLETDESMLLYKCTQALDRYNHQLVIGNLLQTRNKQVIFVSPENRKGDWVRLDEKHHSIEEMIIPEVIARHDKWVAHSKTKLATK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine..2. The protein should be designed to catalyze the following reaction (R)-4'-phosphopantothenate + CTP + L-cysteine = CMP +   diphosphate + H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine.3. A protein that contributes to coenzyme A biosynthetic process by functioning in cytoplasm with phosphopantothenate--cysteine ligase activity.4. A protein that can modulate the overall flux of metabolites through the Cofactor biosynthesis, coenzyme A biosynthesis, CoA from (R)- pantothenate: step 2/5 pathway, enhancing or suppressing its activity as needed.5. helix-helix-strand-strand-strand-helix-strand-turn-helix-helix-strand-helix-strand-helix-helix is the desired arrangement for the engineered protein. The designed protein sequence is ",
    "response#1": "KVTLIIGGGIAAYKALDLIRRIKERGIHVRVVLTQAAQKFVTALAASALSNERAYIDLFDPASEFDAGHIRLARECDLVVVAPATADIMAKMANGHADDLASAILLASDKPILIAPAMNPLMWGNAATRRNVAQLQRDGIRMIGPNAGEMAERNEAGVGRMAEPTEIADAAAAALDRLPHADGVRVVITAGGTREPIDPVRFMGNHSTGKMGVALARAARDLGADTLLIHAPLSVTPPGGIRLAPVRTAQQMHDAVMEHLPTTDVLIGAAAVADYRPTTSADQKIKKLPGQQIITLERTPDILKAVGDMRGAQRVQPKLVIGFAAETNDLDENALGKLEKKNLDMIVLNDVTAPDSGFAVDTNRVTILARGAEPVEIPYGPKEALADVIWDAVVQRL",
    "response#2": "KRILLIVGGGIAAFKSLDLIRRLRERGATVVPVLTKAAEEFVTPLSASALAAQKVYRDLFDLTDEAEMGHIELSRDADLLVVAPATADLMAKMANGHANDLASTLLLATDKRVLIAPAMNVRMWDHPATQRNLTTLTSDGVLVCGPNEGDMACGEHGPGRMSEPLEIVAAVELAALVLGEAALTGRQVVVTAGPTREKIDGVRFISNYSSGKMGFALACAAARRGAEVVLVSGPVALPTPDRVERIDVESAEEMEEAVTRFHQSCDLFIGAAAVADYRPETFRAGKIKKNNETMDIRLVRNPDILAGFAAQKTPGQLAVGFALETEGNEAYALEKLQAKQLDMIAFNTYDGKSSGFEVDTNQITLLTPGARPLTGRQVVVTAGPTREKIDGVRFISNYSSGKMGFALACAAARRGAEVVLVSGPVALPTPDRVERIDVESAEEMEEAVTRFHQSCDLFIGAAAVADYRPETFRAGKIKKNNETMDIRLVRNPDILAGFAAQKTPGQLAVGFALETEGNEAYALEKLQAKQLDMIAFNTYDGKSSGFEVDTNQITLVTAGGEETWPRLPKRQVAERLVARLAAAL",
    "response#3": "KNILLGISGGIAAYKMPMVVRLLKKQEANVKVVMTEAAKRFVSELSLATVSEEPIYTDIFPARLDTAADWTQHISLGEWADALVIAPATANTIARLAAGLADDLLTSSFITLRPNRPRLVFPAMDGEMFHSASVQRNLRQLEEDGCKVIAPESGFLASGQCGTGRMPDPERIEEAAADFFAGQHALVTAGPTIEELDPVRYLSNRSSGKIGYALAETLRDKGAQVTLVSGPTSFSDPEGVEVVRVESAEDMFEAVKQRYDNVDIVFKAAAVSDYTPTEMLPHKLKKQEGELSVTFKRTPDILQYLGEHKSHQYLVGFAAETRDIERYAQDKLERKNADVIIANNVGDRSIGFSSDNNDYTDIFINGQRMSLQDKKVLIGCGATIENIDPVRYISNFSSGKMGNELALCAKRRGAIVTVVCGNVKSDYRDFGINYIDVKTNEQMYEQLNKYFNDTDILLMPAAPVDFKVKNKKDTKIKKIKDCKNYDPIEIEENIDILKNLTKNKKKQFVVGFAAETNSVKEYAISKLKDKNLNLIVANDVSNKNIGFNSDFNKVFILDKNNHIEQLEKMTKYDVADIILDRIIELKRGQ"
  },
  {
    "reference": "MAEPLRTHRVVVVGAGIAGLTSALLLAARGLDVTLVDKAATPGGKMRQVMVDGAPVDAGPTVFTMRWVFDQIFAAAGATVEEHLKLQPLGVLARHAWRGHEPRLDLFADIRRSAEAIGEFSGPQEAQRFLGFCRQARQLYDHLEGPYIRSERPTLGSMVGDLGPRGLMALMQIGPFSNLWRSLSRHFRDPKLQQLFARYATYCGASPWMAPATLMLVAQVELDGVWAVEGGMHAVAKAFSALAEARGVKTRYGCGCEQILVRDGRAVGVRLAGGEEITADSVVFNGDVNALAQGLLGDPPRRATAAVAPARRSLSALTWLVNARTSGFPLVRHNVFFDEDYASEFDDIFRQRQLPRRGTVYVCAQDRTDEGIGSDAPERLLCLVNAPADGDRRPFDHSETDPCEQRSLALMRECGLTIDWSPQTHRLVTPANFERLFPATGGALYGPATHGWMSSFHRASSTSRLPGLYLAGGSVHPGPGVPMAAMSGRLAAETLMAHLDSTSRSRRVVISGGMSTRSATTGGMA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: The preferred substrates are 1-hydroxy-neurosporene, 1-hydroxy-lycopene and 1,1-dihydroxyneurosporene, however the 3,4-didehydrolycopene derivatives such as 1,1-dihydroxy-3,4-didehydrolycopene, 1-methoxy-1- hydroxy-3,4-didehydrolycopene and 1-hydroxy-3,4-didehydrolycopene are also efficiently converted; Catalyzes the introduction of C-3,4 double bonds into 1- hydroxyneurosporene (1-HO-Neu) to yield demethylspheroidene (DMS).2. A protein that can modulate the overall flux of metabolites through the Carotenoid biosynthesis, spheroidene biosynthesis pathway, enhancing or suppressing its activity as needed.3. The designed protein should be optimized for oxidoreductase activity activity in order to improve carotenoid biosynthetic process, chlorophyll biosynthetic process.4. The protein should have a low Km value for A + rhodopin = (3E)-3,4-didehydrorhodopin + AH2. The designed protein sequence is ",
    "response#1": "MGGLASAIRLARAGCEVTLLEAREAPGGRMRTLPSVAGPVDAGPTVLTLREVFDDIFEVCGQKLDHHLTLLPQPLLARHWWLDGSTLDLTTDLEANVEAVAAFAGAREARAFRRFHDLSARLYDAFDRPMMRAARPDLRAIATGALKAPRTWPALLPGMTLDRLLRLFFRDRRLRQLFGRYATYVGGTPYGAPGVLALIWAAEARGVWAIEGGMHRLALALARLADDQGVRLRYGAPVARILRQGGRATGVQLADGRTLPADHIVFNGDPAALLAGCLGDGPQDAVPEDRIHPRSLSAWVWSYAARASGPPLVHHNVFFADDPRREFGPIAAGQMPEDATLYICAEDRSGGQLPDGPERFEIIMNGPPGRPAKPEDFAQCRSRTFDRLRQFGLTFDPVPGETSLTAPSGFASLFPASQGSIYGLSPHGALASLKRPLARTALPGLWLAGGGAHPGAGVPMAALSGRHAAEAILDALD",
    "response#2": "MGGLASAIRLARAGCEVTLLEAREAPGGRMRTLPSVAGPVDAGPTVLTLREVFDDIFEVCGQKLDHHLTLLPQPLLARHWWLDGSTLDLTTDLEANVEAVAAFAGAREARAFRRFHDLSARLYDAFDRPMMRAARPDLRAIATGALKAPRTWPALLPGMTLDRLLRLFFRDRRLRQLFGRYATYVGGTPYGAPGVLALIWAAEARGVWAIEGGMHRLALALARLADDQGVRLRYGAPVARILRQGGRATGVQLADGRTLPADHIVFNGDPAALLAGCLGDGPQDAVPEDRIHPRSLSAWVWSYAARASGPPLVHHNVFFADDPRREFGPIAAGQMPEDATLYICAEDRSGGQLPDGPERFEIIMNGPPGRPAKPEDFAQCRSRTFDRLRQFGLTFDPVPGETSLTAPSGFASLFPASQGSIYGLSPHGALASLKRPLARTALPGLWLAGGGAHPGAGVPMAALSGRHAAEAIVAALEAAP",
    "response#3": "MGGLAAAIGAAAAGLRVTVVEAGDAPGGKARAVPTPGGPADTGPTVLTMRHVLDALFAACGTRAEEHLTLIPLPRLARHFWPDGSSLDLFTDTEANIEAIRAFAGDKEAAAFRRFDHLTTGLWEAFHRSVIAAPKPDLWRIAAATVTRPQLWPALRPGLTMRDLLAHHFKDPRLAQLFGRYATYVGGRPGATPAVLSLIWQAEVQGVWAIREGMHGVAAALARVAEAKGVRFHYGAKAKRIVRKEGRVTAVEIETGVSIPCGACIFNGDPGALRDGLLGDAARASMEKSPRPAPSLSAWVWAFGATPIGVDLAHHNVFFTADPELEFGPIGAGEMPEEPTLYICAQDREMQAPVPEIERFEIIMNGPAGHQPFPQEEAQCRARTFPMLAAMGLTFSPDPETRALTTPALLSRRFPGSLGAIYGGSPEGTLATFRRPLARTGLKGLYLAGGGTHPGAGVPMALTSGTHAARALAV"
  },
  {
    "reference": "MHKNSKRNNNLRVSHTEANSVDAEKEKNESQNNFFELLPAEITFKIFSQLDIRSLCRASLTCRSWNDTIRNSDSLWKPHCMTVRAVCRREIDDDLESGYSWRVILLRNYQKSKVKHEWLSGRYSNICSPISLPEKIMYPMDADTWGEILEAELER",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a specific function in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and should be localized to SCF ubiquitin ligase complex in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "LYLQELASIYSLKRLDAFIDSFVLNHFSTLSFTPDFLQTVSVQKLCIYLSSGQVQHTWEYDLLQAALQWLTQQPEREVHTCRVLENIRFPLFPEDILLQRC",
    "response#2": "FVGGEVSERCLELSDDTCYLDTKNEQWVKETSLPARRSHHCVAVLGGFIFVAGGSFSRDNGGNAASNLLYRYDPRRKQWIKVASMNQRRVDFYLASIEDMLVAVGGRNENGALSSVETYSPKTNSWTYVAGLPRFTYGHAGTIYKDFVYISGGHDYQIGPYRKNLLCYDHRTDVWEERRPMTTARGWHSMCSLGDSIYSIGGSDDHMESMERFDVLGVEAYSPQCNQWTRVAPLLQANSESGVAVWQGRIYILGGYR",
    "response#3": "LYLQELASIYSLKRLDAFIDSFVLNHFSTLSFTPDFLQTVSVQKLCIYLSSGQVQHTWEYDLLQAALQWLTQQPEREVHTCRVLENIRFPLFPEDILLQRQ"
  },
  {
    "reference": "MNDEKEPQGTTPWMKSLAIWLGILLALVLFVSMFDSSTRAAAGETVAYSEFLGHVEEGTVKEVDIGDGVVTGKYTNGSAFRAYAPADPTLVQRLADKNVVFRAQPDEQPSVWMYLLYQSLPFLFILGIGFFVMRQMQKNAGSGAMGFGKSRARMLTQKEGRVTFDDVAGIDEAREELQEIVDFLKDPSKFARLGGKIPKGALLVGSPGTGKTLLARAIAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIVFIDEIDAVGRHRGAGLGNGNDEREQTLNQLLVEMDGFEANEGIIIVAATNRPDVLDPALLRPGRFDRQVVVPRPDIEGREKILLVHMKKTPLAPDVNARTIARGTPGFSGADLANLVNEAALLAARKGKRLVAMREFEEAKDKVMMGAERKSMVMTEDEKKATAYHEAGHALVSLHVPGCDPLHKVTIIPRGRALGVTWNLPERDRYSMTMKQMKARLALCFGGRIAEQIIYGADELNTGASNDIQQATDMARAMVMEYGMSEKLGWLRYRDNQEEVFLGHSVSRNQSVSEETARLIDQEVRRLVEEGEATARKVLTEHLDELHRLATALLEYETLTGEESKRAIKGEDIGRDDGVSRPSTPLAAGGSSIPKSKRPGGGWGDPAPQGA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization.2. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins; Plays a role in the quality control of integral membrane proteins.3. The designed protein should have a binding site that is specific to the target ATP, Zn(2+) ligand.4. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.5. The designed protein have AAA+ ATPase domains with specific sequence motifs that are crucial for its activity.6. A protein that can perform ATP hydrolysis activity, ATP binding, metalloendopeptidase activity, ATP-dependent peptidase activity, zinc ion binding in plasma membrane to support protein catabolic process, cell division, proteolysis.7. The presence of Zn(2+) is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "MVMSEKEKLNTAYHESGHAIVGRLMPEHDPVYKVSIIPRGRALGVTMFLPEEDKYSHSKRYLDSSICSLFGGRIAEELTLGFEGVTTGASNDIERATSLARNMVTRWGLSEKLGPLQYDTDSDEPFLGRSAGQAQTVYSPETAQRIDEEVRNIIDTCYEKAKQILVDNRDKLDLMADALMKYETIDRFQIDDAAYALGEKFIPKGVLMVGQPGTGKTLLAKAIAGEAKVPFFSISGSDFVEMFVGVGASRVRDMFEQAKKQSPCIIFIDEIDAVGRHRGAGMGGGHDEREQTLNQLLVEMDGFEGNEGVIVIAATNRPDVLDPALLRPGRFDRQVVVGLPTVEDYVAILKNRVAEFEAEHGITVDRDVESRTIARGTPGFSGADLANLVNEAALLAARKNRRVVTMQEFERAIKKIIITSK",
    "response#2": "MVMTAAQKEMTAYHEAGHALVGMELPKCDPVYKATIIPRGGALGMVMSLPEMDRLNMFKDECHQRLAMTMAGKAAEIHKYGEEAVSNGPAGDIQQASALARAMVLQWGMSDKVGNIDYSEAVQGYSGSTPGFSVSASTKELVEKEVQKFIQDGYDWALKIIKEKEDQFERLAQGLLEYETLTGAEIKRIAARYRAVREAG",
    "response#3": "VLLVGPPGTGKTLLAKAIAGEAGVPFFSMSGSEFVEMFVGVGASRVRDLFRKAKESAPCIVFIDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNSGVIIIAATNRPDVLDSALMRPGRFDRQITVHLPYLEEGQELHLLRHASQHGMLMEEGFSIEFLAKQTPGFSGADIANVCNEAALIAARKSKLFVEKQDFGRIDTRIIQDKEKTVIAYHEVGHALLGQLLPNMESPHKITILPRGMALGYVLTLPKEDQFLHSKSEMIDSMIMSLGGRAAEQIVFNEVWTGAVSDLQKVTETARRMICEFGMSESLGMLQFGKRSGPVFLGRDFHEERNYSEEIAAKIDAEVRELVDSCYQKAVEMLTSNRAIMDRIVHVLMEKETLSSEELSKVFGLTAQ"
  },
  {
    "reference": "MADEELVKKMVRAVLQSSKSGLSLSDLQVEYKDLTGELIPYKQLGYVTLDALLHSMPSIVKLDKGQSGEVMCHATTGNEMAHITKLAARQRTAKKTGRPQVVNCQMRVKPAAPLVLNAKPRTSLRQPDHRGRLGRGGGRGHGDTRTGCMRDPQLDGKGGRPHNTPPNTPSRKPSLPSERPEKRMTLPSRFQKEVHAHILRNPQHINVPSNLNENTTPPKPRLPHSAPYSPKLVQSRLQEVLNKHSNGLWVSKLPQLYREHYKQDLPSEALKELENWTHICTVEKPCSSKPQELLLYPAKETSQITPTPTHDRSPSHHKKPQTQRPSLTAKSNTPESPKALSPDLKQKLGELLLKYSSGLWAHALPKLYQDTYKCKLPEFVLDQLTLLSDICTIDYPVPDNPKRAILYAKVVEDENRNQSGLAGQEMKLERRLSSQTVPPLVIPRVEYPSVLVVDASDTNCVILRYIGEGYSKAQEKLEDEMREFYRQDNTKMALRSPSPGQLTAVGAEEEDDIIRAQVCEVMADKVKVYYVDHGFSEVISIRKLFELNEKFYRLPFQATKCKLAGLESFSQEQAVLKKLESIATGKILLAEILEREDMPLVVLYDTSQDDDVNINAACMKALQDRSLESPLKVNSAYMNVSVTSVCSDGTIYCQVPSRGLTKLNEILEKTENYFHSQVTSESLVSRPFCGKNCLARYKGKWSRVEITNLHGSRVLDILFVDVGVQASVEVIELREIPPPLLRDLISTPAQALKCCLAGLPVSVGLWTP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Probably required during spermatogenesis .; Component of specific cytoplasmic RNA granules involved in post-transcriptional regulation of specific genes: probably acts by binding to specific mRNAs and regulating their translation.2. The designed protein have HTH OST-type domains with specific sequence motifs that are crucial for its activity.3. The designed protein should be optimized for mRNA binding activity in order to improve piRNA processing, P granule organization.4. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "QGSGVCKWFNVRMGFGFLTMTKKEGTDLETPVDVFVHQSKLHMEGFRSLKEGESVEFTFKKSSKGLESTRVTC",
    "response#2": "CHFTVFMALSKMKMPLHELQAHVRQNPIQNVEMSFVDAPEKNGIVTTYLRITAREKNQHELIEAAR",
    "response#3": "MGVPAFYRWLADRYPLSIVDMVEEEPKDDVPVDISKPNPNGMEFDNLYLDMNGIIHPCFHPEGKPAPATYNDVFNSIFDYIDHLFSLVRPRKLLYMAIDGVAPRAKMNQQRTRRFRASKDAAESEAEEKRLREEFEMEAAILVPTEKPETSDSNVITPGTPFMAVLSIALQYYIHSRLNKNAGWRFTKVILSDANVPGEGEHKIMSYIRLQRNIPGFNPNTRHCLYGLDADLIMLSLATHEVHFSILRR"
  },
  {
    "reference": "MIELLFLLLPIAAAYGWYMGRRSAKKDQDDISNKLSRDYVTGVNFLLSNQTDKAVDLFLDMLQKQEIENEIESHSQFEAELTLGNLFRSRGEVDRALRIHQALDLSPNYTFEQKLLAKQQLARDFMVVGFFDRAENLYILLVDEPEFAENALQQLLVIYQKTKEWKKAVNIAEKLAKIKPQENNIELAQCYCEYSQSLEPESAVEKRSVLQKALSVSPTCVRASLLLANLAMLDGQYQQAVKILENVLEQNPDYTGEILLPLKHCYEELNQLDNFELFLIRAGQIINNDEVELALAKLIEEKDGKSAAQAKLYQQLTKKPSTLIFHRFMQYQIDDAEDGRGKESLILLHKMVGERIKQTSPYRCTNCGYQIHKLLWNCPSCRQWESIKPVSNQEHN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have a high specificity for Fe cation binding, with minimal off-target effects.2. The protein must exhibit the following characteristics: May also coordinate assembly of proteins involved in LPS synthesis at the plasma membrane.; May act by modulating the proteolytic activity of FtsH towards LpxC.3. The designed protein should have a specific function within the plasma membrane. The designed protein sequence is ",
    "response#1": "RFASLGDWGKDTKGQILNAKYFKQFIKNERVTFIVSPGSNFIDGVKGLNDPAWKNLYEDVYSEEKGDMYMPFFTVLGTRDWTGNYNAQLLKGQGIYIEKNGETSIEKDADATNYPKWIMPNYWYHYFTHFTVSSGPSIVKTGHKDLAAAFIFIDTWVLSSNFPYKKIHEKAWNDLKSQLSVAKKIADFIIVVGDQPIYSSGYSRGSSYLAYYLLPLLKDAEVDLYISGHG",
    "response#2": "ISFVLFIAICMSLTRGAILVAALIYALYIISNRKMLFCGITLFVIIIPVLAISTNIFDNYTEILIGRFTDSSQASRGSTQGRIDMAINSLNFLSEHPSGIGLGTQGSGNMLSVKDNRLNTDNYFFWIALETGIIGLILF",
    "response#3": "VGLGALLIGPVLLVNVILKEHWGRPRPVQTDIFGGALHFVEAGSLAGKCVSNCSFVSGEAASAGWLFCLLLFVPKSLRYALVPPVAAISILTPAMRLSFGAHYLSDVTLGWLSSLVVFAALF"
  },
  {
    "reference": "MGLIFQPLFWQYVATSYALMVLGFLDETVEKAIKSEGYCSRILRAQGGTRKEGYNEFSMRVEGDPEFYKPGNTYRVTILAVSPAYFRGFTLIALKEGKEGEKEEDHAGSFQIIDEEDTQFMSNCPVAVTESTPRRRTRIQVFWTAPSIGTGCVILKASIVQKKIIYFQDEGSLTKRMCELDLTLEGGNEKTIPDCCACGTAKYRLTFYGNWSEKAHPKDYPRRANHWSAIIGGSHSGEYVLWEYGQASDGVKQVAELGSPVKMEEEIRQKGDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRSRHLMSFLAMMGPSPDWNVGLTSEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQDKIRPLTSLDHPQSPSMTRGGPIIPIARVVIERIARKGEQCNIIPDNVDDIVADLVTEEKDEDDTPETCIYSNWSPWSACSSATCDKGKRMRQRMLKAQLDLSVPCPDTQDFEPCMGPGCSDDEASTCMMSEWITWSPCSASCGMGIEVRERYVKQFPEDGSLCKVPTEETEKCIVNEECEPSSCIVTEWAEWEECSATCRMGMKKRHRMIKMTPADGSMCKADTTEVEKCMMPECHTIPCVLSPWSEWSDCSVTCGKGTRTRQRMLKSPSELGDCNEELELKQVEKCMLPECPISCELTEWSYWSECNKSCGKGHMIRTRMITMEPQFGGAVCPETVQRKKCRLRKCQKSSGNERRHLKDAREKRRSEKIKEDSDGEQYPVCKMKPWTAWTECTKFCGG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro.2. The protein should be optimized for stability and expression in extracellular region where it will participate in cell adhesion.3. The designed protein have Reelin domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "PTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTPDSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDETLSFIKSHPLMDSAVPPIADEPWFTKTRVRYRLTAIAVDHSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNHC",
    "response#2": "CQPKVMRLKDYVKVKVEPSGITCGDPPERFCSHENPYLCSNECDASNPDLAHPPRLMFDREDEGLATYWQSVTWSRYPSPLEANITLSWNKSVELTDDVVVTFEYGRPTVMVLEKSLDNGRTWQPYQFYAEDCMEAFGMSARRARDMSPSSAHRVLCTEEYSRWAGSKKEKHVRFEVRDRFAIFAGPDLRNMDNLYTRMESAKGLKEFFTFTDLRMRLLRPALGGTYVQRENLYKYFYAISNIEVIRCRIYGTACADCCLARDPYCAWDGNSCSRFYPTGKRRSRRQDVRHGNPLTQCEPSATCCACDIAQYDLEFTGIWSKNTHPKDYPTLEHLTHFTDMLGSSHSSNYSLWTIGGISTDGMKEIAEWGNTYKAEAEAKAKASEVRSLMKVKGLWFPDVQGTTKSQFVVNKYHHFVSLATMFGPSPDWCVGLSSVNLCLPDCTWAEERTFELQPFDAGTDSGPTYMSPNEPTEPREPIHWITTKLNPLSC",
    "response#3": "CQPKVMRLKDYVKVKVEPSGITCGDPPERFCSHENPYLCSNECDASNPDLAHPPRLMFDREDEGLATYWQSVTWSRYPSPLEANITLSWNKSVELTDDVVVTFEYGRPTVMVLEKSLDNGRTWQPYQFYAEDCMEAFGMSARRARDMSPSSAHRVLCTEEYSRWAGSKKEKHVRFEVRDRFAIFAGPDLRNMDNLYTRMESAKGLKEFFTFTDLRMRLLRPALGGTYVQRENLYKYFYAISNIEVIRCRIYGTACADCCLARDPYCAWDGNSCSRFYPTGKRRSRRQDVRHGNPLTQCYSPSATCCACDIAQYDLEFTGIWSKNTHPKDYPTLEHLTHFTDMLGSSHSSNYSLWTIGGISTDGMKEIAEWGNTYKAEAEAKAKASEVRSLMKVKGLWFPDVQGTTKSQFVVNKYHHFVSLATMFGPSPDWCVGLSSVNLCLPDCTWAEERTFELQPFDAGTDSGPTYMSPNEPTEPREPIHWITTKLNPLSCE"
  },
  {
    "reference": "MAADATKGPPGGGEGEVHAGSGPGPYQDEIVISWPELHRDARYLSRVLHELGDWKGIIAITRGGLVPAALVARELDIRLIDTVCVVSYGAAETGGAEAKQGELQWLKTVPGDGEGWLLIDDLVDTGRTAAAVREKLPKAHFATLYAKPLGRPVVDTFVKEFRQEKWIYFPWDIDYRFVSPIRQRGAKD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: To a lesser extent, also acts on hypoxanthine..2. The designed protein should contain one or more Phosphoribosyltransferase domains that are essential for its function.3. The protein must be able to magnesium ion binding.4. Ideal protein should have a binding affinity for key enzymes in the XMP from xanthine: step 1/1, XMP biosynthesis via salvage pathway pathway to enhance the overall efficiency of the pathway.5. The 5-phospho-alpha-D-ribose 1-diphosphate binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "LMQAMGEEFATIFSDEKIDKIVTVESSGIAPAVFAGLALHVPVVFARKNKSLTLPENVWTADVYSFTKQTTNHIMIDHRFLSAAENILIIDDFLANGQAVEGLLKIANDANANVVGVGVVIEKS",
    "response#2": "SWDQLHRDSKALAWRLVEAERPWRGIVAIARGGLVPAAIVARELEIRLIDTFCVASYDDRRQGEARILKAATAALVAAHPGASDEQAGAGWLIVDDLVDTGQTARIVREHLPGAHFATVYAKPAGRPLVDTYVTEVSQDTWILFPWDTELY",
    "response#3": "EIAYEFMDRFKDAGITRILTIEAGGIAPAMMTALRLGVPMVYARKTKSLTLNEGTISAEVYSFTKQQTSTITVAEQYLQAGERVLIIDDFLANGEAAFGLAKLVEQAGAEVAGFGIVIEKSFQPGRQKLLDAGFRVESLG"
  },
  {
    "reference": "MSLLDPIEDAIAAIAAGRPVVVVDDEDRENEGDIVFAAEFATPELMGWTVRYTSGVICCPMPGNRADALGLPPMVTDNQDAKGTAYTVSCDAAAGVTTGISGADRATTARLLADPGSNSAHITRPGHIFPLRSVEGGVLARRGHTEASVDLCRLAGLEPVGVIAELVHDDGTMMRLPALREFASQWEIPLVSIEDLAAHLADAGALAPEPTAPAAVLGGPVVQLPTAHGTFAVQAWTDRASGDEHFTLSMPSVHASPLVRLHSECLTGDIFGSYRCDCGEQLDSALRAIAAEGGTVVYLRGHEGRGIGLANKIRAYALQDGGADTVEANEQLGLPVDARRYDAAAAILHSLGLERIRLITNNPLKSRWLQQAGIEVAGVISSAIDARPENERYLRTKVDRMSHSIDPAAFGAGTPE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.2. The designed protein must possess zinc ion binding, GTP binding to support riboflavin biosynthetic process under certain conditions.3. The designed protein should contain one or more GTP cyclohydrolase II domains that are essential for its function.4. For general function, the protein need meet that Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.5. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.6. The Mg(2+), Zn(2+), D-ribulose 5-phosphate, GTP binding site should be located in a region of the protein that is accessible to the ligand.7. A protein should be able to manipulate the activity of enzymes in the riboflavin biosynthesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "MSASKLPTPFAEFAMHGFIESATGREHVVLSLGDVADGAPVLGRVHSECLTGDALFSQRCDCGSQLEAAMRAIATEGRGVLLYLRQEGRGIGLMNKIRAYELQDGGADTVEANERLGFAADQRDYAICLPMLQHLGVQSLRLMTNNPRKVKALTDMGINVAERVPHPEVPRGKDERAAEAKLPTSFAGEFKIIAYTNDIDDYVHIALVKGEIDSNEEILVRVHSECLTGDVFGSLRCDCGDQLRAAMKQIDREGKGVLLYIRNHEGRGIGLLNKIKAYKLQENGLDTVEANHVLGFKEDLRDYGIGAQILVDLGIRKMRLMTNNPKKIVGLEGYGLTVTERVTSRLPTRFGEFTVTAYDNPSYPDPDLALTMGDISTGEPVMVRFHSECLTGDVFGSTRCDCGDQLRIAMERIAESGRGVILYMRQEGRGVGLTNKLRAYHLQDRGMDTVEANEHLGFPADLRTYGPGARILQDQGLHRIRLLTNNPDKVAGLEAHGLEVVERVPQGE",
    "response#2": "MIASKLPTPFAEFAMHGFIESATGREHVVLSLGDVADGAPVLGRVHSECLTGDALFSQRCDCGSQLEAAMRAIATEGRGVLLYLRQEGRGIGLMNKIRAYELQDGGADTVEANERLGFAADQRDYAICLPMLQHLGVQSLRLMTNNPRKVKALTDMGINVAERVPVADVRTAVARAAEAKLPTSFAGEFKIIAYTNDIDDYVHIALVKGEIDSNEEILVRVHSECLTGDVFGSLRCDCGDQLRAAMKQIDREGKGVLLYIRNHEGRGIGLLNKIKAYKLQENGLDTVEANHVLGFKEDLRDYGIGAQILVDLGIRKMRLMTNNPKKIVGLEGYGLTVTERVPQSPG",
    "response#3": "MSASKLPTPFAEFAMHGFIESATGREHVVLSLGDVADGAPVLGRVHSECLTGDALFSQRCDCGSQLEAAMRAIATEGRGVLLYLRQEGRGIGLMNKIRAYELQDGGADTVEANERLGFAADQRDYAICLPMLQHLGVQSLRLMTNNPRKVKALTDMGINVAERVPSATLSGKKGQERVTTVKLPTDFGIFDLHCFVAKHDGREHLALVYGEVAGKQNVLTRVHSECLTGDVFHSQRCDCGAQLAVAMQRIVAEGSGVLVYMRQEGRGIGLANKLHAYHLQERGLDTVDANVRLGFPPDLREYGIGAQILGNLGVHSIRLLTNNPKKLVGLEGYGLSISGRAAEAKLPTSFAGEFKIIAYTNDIDDYVHIALVKGEIDSNEEILVRVHSECLTGDVFGSLRCDCGDQLRAAMKQIDREGKGVLLYIRNHEGRGIGLLNKIKAYKLQENGLDTVEANHVLGFKEDLRDYGIGAQILVDLGIRKMRLMTNNPKKIVGLEGYGLTVTERVPIKV"
  },
  {
    "reference": "MDLAAFVDRLRNGSAAKFPADANTLAFAQKLDSQDQLKHLRDEFVLPTKGSLKKRALDGSIPGQAANNGINGTHNTNGAGGQSADADKPCLYFVGNSLGAQPRAVRDYLNAQLETWASIGVNGHFTDMDNSPLRQWQDMAEDCAKKSSDLVGASPHEIVIMNTLTVNLHVMMASFYKPTAKRHKVILEWKPFPSDHYAIESQVVWHGRDPDKSMVKIEPDENSLIPTEKILRTIDEHAQETALLLLPGIQYYSGQLFDMARITAYAKERGIVVGWDLAHAAGNVELRLHDWDVDFACWCTYKYINAGPGSIAGAYVHERHGQVEWAGGSGRPSYRPRLAGWYGGDKSVRFNMDNTFVPTPGAAGYQLSNPSAIDLAALSGALSVFNKTCMRDLRSKALVLTAYAELLLDQMLEETKGDAAPIFTVLTPRDPLQRGTQLSVLLRDGLLEKVSQALEENGAICDKRKPNVIRVAPVPLYTRFEDVWAYMQVLRGALGL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein have Aminotransferase class V domains with specific sequence motifs that are crucial for its activity.2. The protein must bind to pyridoxal 5'-phosphate in order to perform its enzymatic function.3. A protein that is capable of catalyzing reactions within the quinolinate from L-kynurenine: step 2/3, Cofactor biosynthesis, NAD(+) biosynthesis pathway with high efficiency and specificity.4. A protein that can perform 3-hydroxykynureninase activity, kynureninase activity in cytoplasm to support quinolinate biosynthetic process, anthranilate metabolic process, L-kynurenine catabolic process, 'de novo' NAD biosynthetic process from tryptophan, tryptophan catabolic process.5. The pyridoxal 5'-phosphate binding site should be stable and able to withstand changes in environmental conditions.6. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.7. The protein must exhibit the following characteristics: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively.. The designed protein sequence is ",
    "response#1": "MNAKKLMARMVGAQEHEIAILNGLTVNLHLLLATFYRPDSNRYKLMIEDHAFPSDTYVAKSQLRSHGISPEKGLILLKPRKGENLLNEDDIIRLFDEQGDQIKVALLPAVQYYTGQLLNVKRLTEAAHKKGILVGVDLAHAVGNVPIYLDEWNVDFAAWCTYKYMNAGAGSMGAIYVNEKYFTNYSETFPMYQGWWGNNVETKFLMKGDFDPALGADMFKLSNPSPVLNAMLMTSLEIFEQTSMEELRQKEYLLTGYLPGAIKEQTAKILGALPTEVVIMETLTANLHLLMSSFYRPTKDRWKIIIEGKAFPSDHYAVLSQIANHSLDPSALITIDPPSASSPYLSNEHILSVISRHAATTALVLLPGIQFYSGQFFDIELITRHCQSLGITVGWDLAHAAGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHNDAYNYDDGPDAIKEQTAKILGALPTEVVIMETLTANLHLLMSSFYRPTKDRWKIIIEGKAFPSDHYAVLSQIANHSLDPSALITIDPPSASSPYLSNEHILSVISRHAATTALVLLPGIQFYSGQFFDIELITRHCQSLGITVGWDLAHAAGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHDDAYNYDDGPDDDATKQQSAKIVGALPSEVVVMETLTANLHLLMSSFYRPTADRWKIIIEGKAFPSDHYAALSQIAHHNLDPSALITIEPPSASSPYLSTEHILSVISKHAATTALVLLPGIQFYSGQFLDIELITRHCHSNGIPIGWDLAHAVGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHE",
    "response#2": "MATKQQSAKIVGALPSEVVVMETLTANLHLLMSSFYRPTADRWKIIIEGKAFPSDHYAALSQIAHHNLDPSALITIEPPSASSPYLSTEHILSVISKHAATTALVLLPGIQFYSGQFLDIELITRHCHSNGIPIGWDLAHAVGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHEP",
    "response#3": "MSLAKKLMARMVGAQEHEIAILNGLTVNLHLLLATFYRPDSNRYKLMIEDHAFPSDTYVAKSQLRSHGISPEKGLILLKPRKGENLLNEDDIIRLFDEQGDQIKVALLPAVQYYTGQLLNVKRLTEAAHKKGILVGVDLAHAVGNVPIYLDEWNVDFAAWCTYKYMNAGAGSMGAIYVNEKYFTNYSETFPMYQGWWGNNVETKFLMKGDFDPALGADMFKLSNPSPVLNAMLMTSLEIFEQTSMEELRQKEYLLTGYLPAIKEQTAKILGALPTEVVIMETLTANLHLLMSSFYRPTKDRWKIIIEGKAFPSDHYAVLSQIANHSLDPSALITIDPPSASSPYLSNEHILSVISRHAATTALVLLPGIQFYSGQFFDIELITRHCQSLGITVGWDLAHAAGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHEST"
  },
  {
    "reference": "MLDAQTIATVKATLPAIAKLGPQLTGHFYQRMLSQHPELKNVFNMNNQRSGNQREALFNAICAYGSNLENLEVLLPAVEKIAQKHTSLNIQPEQYAIVGENLLATIKELMNPGDEVLDAWGKAYGVLANVFIQREEAIYRASEEKVGGWRGARDFRIRAIEQQSAVIKSIELIPEDGQPIADFLPGQYLALSLRPQDSEHIQHRQYSLTHLPNGKSYRIAVKREAHGSVSGWLHQHAKVGDIVQCTAPAGDFFLQAEPTTPVTLISAGVGQTPMLAMLAALADQQHPAAVTWLHAAENGAQHAFADEVVELGARLPNFRRHVWYRQPESTDAGRFTARGLMDLAAVSAGLNQPDRQFWICGPLPFMQFVARQLVDAGIHADRIHYEVFGPHKVL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Charge relay system active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.2. The protein should be able to bind FAD, NADP(+), heme b ligand in a variety of conditions3. For general function, the protein need meet that Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds4. The designed protein must possess metal ion binding, oxygen carrier activity, heme binding, nitric oxide dioxygenase activity to support response to nitrosative stress under certain conditions.5. The protein contains novel FAD-binding FR-type, Globin family profile domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MLSQQHIDTVKATIPLLASAGTAITEHFYNRMFTHNPELKNVFNMTHQKTGRQPAALFNAIAAYATYIDNLEALTSAVMRIAHKHTSFNIQPEQYDIVGHHLIETLRELAPDAFTKEVEEAWTAAYVQLAEIFIEVE",
    "response#2": "MLSENTIKIVKSTAPVLAERGVEITSYFYKTMFEAHPELLNIFNHANQKQGRQQTALANTVYAAATYIDQLEVLLPAVKQIAHKHRSLNVKKEHYPIVGEFLLKAIKDVLGDAATPEVLQAWGEAYQVIAQVFEGFRRFRVASKTPESELVTSFVLEPVDGQAVIDFQPGQYLGVKLHPKGAEFDEIRQYSLSTMANGKSYRISVKREGTGDTAGVMSNYLHNHIEVGDEIDVMPPAGDFHFLISGGVGLTPLTSMLETVVTQQPNREVYYIHAAQHERVHGLKDTVKQIADQHDHVHTFTIYEKPEDANACDKTGFIDLAWLQSVLPTNQASFYFCGPEPFMKAINETLRQWGIPPERVHYEFFGPAGSL",
    "response#3": "MLDSRTIEIVKSTVPLLESANTALTEHFYKRMFRDNPELKDVFNLSHQKTGRQPAALFAAVLAYAKNIDNPAVLSAAVERIAHKHTGFSIQPAQYDIVGHHLLETIKELAPDAATPEVIDAWAQAYGALAGIFIGVETEMYQEAEQRKVKESDVITSFYLVPADGQPISSYEPGQYVSIKVELPGEQFTHIRQYSLSDAPGNAYYRISVKREDAMQDKPAGKVSVYLSEEVKEGDSLQLSAPAGDFRVDEKVTESNEVTSFYLKPADGKSIASYEPGQYLTLKADIEGETYTHMRHYSLSDAPGQDYYRISVKREEGEGSTPDGIVSNHLHRNVKKGDTLAFAAPAGDYRTADGEPVVLLSGGVGLTPMISMLNTLVEESHPSRTITFIHAAQNGEVHAFREHVNALAEQHPQVSVHYCYTRPTEADRNQGHFDKEGYIDLAWLQSVIPSPADFYVCGPVPFMKTINGQLEEWGIPASDIHFEFFGPAASLQPV"
  },
  {
    "reference": "MVGMGDRLFNMVFTRRPPQSMSRCISDPGYRAAARFSYEEASAQYKVYVERLHSAGITVKELGPLEDYPDSVFIQDTAVIGGGSRVAVLARFGAPSRRGEEGHVVSILSSMGLEIHPVKPPGTLEGGDVLVTGEGVVFAGLSSRTNREGVETLKTAFPNVNVETLNAKGLHLLSHLGYLGKATLISAEGLYDKSIFKRHGFDLIEIPWEERDAANLLYLGEGRVLLPAGYNQTRDLLEQHGFRIVEAEIRQFMACMGGVTCLSLPIYNIL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must have a Nucleophile that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "LLDSYKSVLEAFIHSGVENSARVNLKWIKTEKIKNEEKGKSEFKDVDGILLLPGFGSRGGEGKIFCAEYARANKLPFLGICLGLQCAVIAFARNVCGLKDANSTEFNKKTKYPVIDLMESQRAIKIKGGTMRLGSYECKIKTGTKTYASYRKKIVSERHRHRYEVNNRYRKKLEKNGLVFSGINEDLGVVEIIEIPNHPWFVAGQFHPELKSRINKAHPLFRNFIY",
    "response#2": "TESYKSLNEALVHGGIANDTHVNLKFVNSTDLQEEQGDISKYFEDIHGVLVPGGFGVRGTEGKILAIKYARENGIPLFGICLGMQLAVVEFARHVCNMENANSTEFDADTPWPVIDLLPGQISIERKGGTMRLGAYKCVLTPNTRAMEAYKSNVISERHRHRYEFNNKYEETLVEHGLVVSGKNPETSLVEIVELKDHPWFLGCQFHPEFKSKPMNPHPLFREFIG",
    "response#3": "LLDSYKSVLEAFIHSGVENSARVNLKWIKTEKIKNEEKGKSEFKDVDGILLLPGFGSRGGEGKIFCAEYARANKLPFLGICLGLQCAVIAFARNVCGLKDANSTEFNKKTKYPVIDLMESQRAIKIKGGTMRLGSYECKIKTGTKTYASYRKKIVSERHRHRYEVNNRYRKKLEKNGLVFSGINEDLGVVEIIEIPNHPWFVAGQFHPELKSRINKAHPLFRNFIY"
  },
  {
    "reference": "MADLPVPGESGRVSGMFGRVAGSYDLLNHLLSGGLDIAWRRAMVRALLAPGLPPGPLLDLAAGTLDVSIMLARHGDRTIVAADPCRPMLTRGVAKLTAETRGRIHPVEGDGLSLPLADASVAGATIAFGIRNIRPRPEAFAELARVIRPGGRLMVLEFGSGKRRLWGGCYNFYLRRVLPVIGKIVSGDDEAYRYLAATISEFPHEDALAAEMRQAGFRDVTYRAMAGGIVFLHAGVKGA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. We require a protein that can specifically bind to the metabolites produced in the Quinol/quinone metabolism pathway.2. A protein with 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity, S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity functions is required to enable aerobic respiration, methylation, ubiquinone biosynthetic process. The designed protein sequence is ",
    "response#1": "LIVTFLAG",
    "response#2": "GFATNPRV",
    "response#3": "MNEDWE"
  },
  {
    "reference": "MATRGSGTRVQSQPKIFPSLLSKLHGAISEACVSQRLSTDKKTLEKTWKLMDKVVKLCQQPKMNLKNSPPFILDILPDTYQRLRLIYSKNEDQMHLLHANEHFNVFINNLMRKCKQAIKLFKEGKEKMFDENSHYRRNLTKLSLVFSHMLSELKAIFPNGVFAGDQFRITKADAADFWKSNFGNSTLVPWKIFRQELNKVHPIISGLEAMALKTTIDLTCNDFISNFEFDVFTRLFQPWVTLLRNWQILAVTHPGYVAFLTYDEVKARLQRYILKAGSYVFRLSCTRLGQWAIGYVTAEGEILQTIPQNKSLCQALLDGHREGFYLYPDGQAYNPDLSSAVQSPTEDHITVTQEQYELYCEMGSTFQLCKICAENDKDIRIEPCGHLLCTPCLTSWQVDSEGQGCPFCRAEIKGTEQIVVDAFDPRKQHNRNVTNGRQQQQEEDDTEDIGDFNIATSSLHALSTSSTVAAEKHSPHTSPRLGRRSTTPSLMAVQNDLYAGGTPTLSLPSSSCSSIAAASTSSSSSLASVATVAASTSSSQHQQPQPSAPPASAVLSNGGGGGGGGGASSSQKNTNRMSAPLIGSCVANSTYGQKLPQNCSHSSTSSSSDNASSSSSYAILQNLHDTGTPATAAAVVAPPLPPRKSSPGVETPSKATAPPPPSSSKSIDNIQCSLDNVPPQTTAPPIPPHVSPSVDTLAEDLMRQQLIATSTTSPVLSLLDEDIVEVGPAETISGVIDTRPLEARGVTLCRQDSASSHYTQLCTTSSGG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a low Km value for S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +   [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-   cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine..2. A protein that can modulate the overall flux of metabolites through the protein ubiquitination pathway, enhancing or suppressing its activity as needed.3. A protein that contributes to eye photoreceptor cell differentiation, dorsal/ventral axis specification by functioning in cytoplasm with phosphotyrosine residue binding, zinc ion binding.4. The designed protein must have at least one UBA, RING-type domain.5. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "LGPFFSYGLESSARRPNHRVFVDCEEDARKYDGSVNTEQKLYFQRMDPIRTMLHQLMLPLASDQGSRNKTLRWIATIISTNDIRTRSHYDPSDVLCDHYMTNFLSVMYMFSEKIDLSKIIVDYPFLPSSLINISKETRLKMDESGAVAFASQFADRPDEYHFSTVCFFLTIAAQRLVIPPLMNQISEYSRHLKELKHKINALKEKLNTVSGFERAEVEKKLNYETEHWKLMSRHLLCVKTQAQDPALMASSMDFVDKQMKFILNLLCDNLDLLGDDSQLPTEVSQMFCALPEYFLEDALDFYIFAISNGMKLLMERNADWISRLTVLFTQYHYIKSPFLVSKLVRVLSSIQPPLWFNVVRLRMAQENLLMCMIKFYSDFEDNGDFYEKFNVRGNIQYMLEKMEEDMFYKGKFMDMARECGAEFIRFVNMVINDATWCIDESLSGLKSIHDVEKKMANKVEWDNTDQEIRNQDLGVYEEAKRKVKGA",
    "response#2": "VDKKMVEKCWKLMDKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTILSRYEGKMETLGENEYFRVFMENLMKKTKQTISLFKEGKERMYEENSQPRRNLTKLSLIFSHMLAELKGIFPSGLFGR",
    "response#3": "VDKKMVEKCWKLMDKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTILSRYEGKMETLGENEYFRVFMENLMKKTKQTISLFKEGKERMYEENSQPRRNLTKLSLIFSHMLAELKGIFPSGLFV"
  },
  {
    "reference": "MEQIKLNTIEEAIEDIKNGKLLIVVDDEDRENEGDFVCAAELITPEIVNFMATHGRGLICAALPEERCEELELDLMVGINTSLHETQFTVSVDLLGPETTTGVSARDRALTIKALVDPKTKPSDLGRPGHIFPLKAKTKGVLRRAGHTEAVVDLTRLAGLNPGGALVEIMNDDGSMARLPDLEMLKNKFNIKIVAIRDLIRYKLERDSIIEKGEKVKLPTEYGEFEFIPFRQTSNGLEHAALVKGSWTRDEPVLVRLHSSCFTGDIFGSMRCDCGPQLHKAMQMVEAEGKGVVIYLSQEGRGIGLFNKIKAYKLQDEGMDTVQANLKLGFGEDERDYGVGASIMRELGLGKVRLISNNPVKRAGLEGYGISIVETIPLVIQSNPYNEFYLRTKASKMGHNLTCHKHIVKD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must have high specificity towards GTP binding, GTP cyclohydrolase II activity.2. The designed protein should be able to compete with endogenous proteins or inhibitors for D-ribulose 5-phosphate binding.3. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.. The designed protein sequence is ",
    "response#1": "VANLPTEFGQFKIYAYHNTLDGSDHVAIVKGEPEEFEDKPTMVRVHSECLTGDALGSLRCDCRMQLQAALKMIENNGSGIVVYLRQEGRGIGLINKLKAYSLQDIGFDTVEANERLGFPADLRNYGVGAQILNDLGVSKIRLITNNPRKIAGLKGYGIEIVGRVL",
    "response#2": "RVAETRLPTEFGEWRCVAYKAITDPDEHVALVHGEVDGEEPVLVRVHSQCVTGDVFGSKRCDCGEQLHMAMQMIEEAGKGVVVYMRQEGRGIGLHNKIKAYNLQDGGMDTVEANEALGFPADRRDYGIGMQILVDLGLRKLRLLTNNPAKRAGLEGFGLEIIERVG",
    "response#3": "ERVSEARVPTKYGVFTAVSYNSLVDRHEHVALVKGEIYPDKATLVRVHSECLTGDVFGSIRCDCGDQRDLALKAIGKEESGVFLYMRQEGRGIGIHNKLKAYALQDDEGLDTVDANIRLGFPPDARQYGVGAQILVDLGVRKMRFLTNNPKKRVGIESFGLELVEQVL"
  },
  {
    "reference": "MDKFWWRAAWGLCLVPLSLAQIDLNITCRYAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMQKALNIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFEGPITITIVNRDGTRYTKKGEYRTNPEDINPSTPADDDVSSGSSSERSTSGGYSIFHTHLPTTRPTQDQSSPWVSDSPEKTPTTSTDSNVISAGWEPTEENEDERDKHPSYSGSGIDDDEDFVSSTIPTKPRIFKFPEVNEDWRQWSPGHSTPGILLQTATRMTDVDRSGTSAYGESQTQEPHSPLVHHEHHDEEETQHSTSSTWALSSRTAEDTATQKEQWFEDGWRGEYTQTPKEDSPSATGTAVTTASAHDSHPNRRMTTQSQEDSSWTYFFDPISHPMGRGHHTERWMDVDSSRSTTLQPSADPSTGLVEELDRTRPLSMTTQQSHTQSFSTSHGGLEEDKDHPMTSTPSSSNRNDGRGGRRSGNFSEDSATSGEGYTAHAPDTKEYNTLTPVTPAKTGSPGVTEVIVLGDSNSHVDGSFSEDRASGGRAQTTHGSETSGHSTGSQEGGASTTSGPIRRPQIPEWLIILASLLALALILAVCIAVNSRRRCGQKKKLVINNGNGAVDDRKASGLNGEASRSQEMVHLVNKESSETQDQFMTADETRNLQNVDMKIGV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a specific function within the Golgi apparatus. The designed protein sequence is ",
    "response#1": "QTAAIDIMEEMTLHQEGALERLYRWTQNHCRSLENNEIGPLIVEAMSRLQDRPVLFKYVIDEYAIARRAVLVRQFIEALTEGGPGGNPKPIELHAHDPKRYIGDMFAWLHQSIPTEKENLSLLFKKCDKQDISDQLQNALGYIADGVCHPLKVRVETILQAEKDTIVLFTISNLLRFYQQIMRQVVQGGSLEECLVELQKSSEQIYLGALAAQVRSVLQRPSGGSGLALEPPQRDLVPPPSVARLLNMLKEILSVATMVDGRQADITKIVSCVIDPLLQSVQESAAHLPTVDMGVYLLNCLHHMQSSLAVYEYMDERVERLQAQSDAQLDTLTSEQASSLVANLNLGPIYAILQSNQSKIETNLLKIFMSKMDAFLELPDVLLLPQVQLIMSSSHRAAVQKRSFNVIVAIYKQIYERVHDPANGFEQPEQLLHRTPEQVAHII",
    "response#2": "QLGLKIMSKFNQLINKSVERIINFTNKTLNNLYSLNTTTKLTTLHQCLKFLRKRLNYFNTVIDNFVDTRSRLIIDEFLNQINGDLDKSGDNRSGSIQHDRPIILSAHDPVRFIGDLLAYIHSVVVNETETISSIFGISKEENEDDDIEYKDIIDDVTGRILNSLSRSVKSKIEQVITSETKLATIYAIYSLVELYTIMFAKHLKESSNDKHNLLMTVRELVSSSQNKIISIIQNKLTTIKSSNSAQLELNTDLQPPEWIIEFYSDILPIIDQNTSDTFMNLSTENNAEFMKMIINKPIEIFNEHIENNISKLFNRRDQVILKLNFLDLILSKIMPIILLSDKILELNDLTEKLSLELTNLQLDSLISGCQLTNFYNIINMICPFSDDFFDVSIYQPITENKLFSEDNIIAANDIIQNFLPSALLDIQQSLFKVNSPMIVNNIITNSSLEFIKFYLKFNLIINEYLDDASLIWSDIEVATLI",
    "response#3": "QTAAIDIMEEMTLHQEGALERLYRWTQNHCRSLENNEIGPLIVEAMSRLQDRPVLFKYVIDEYAIARRAVLVRQFIEALTEGGPGGNPKPIELHAHDPKRYIGDMFAWLHQSIPTEKENLSLLFKKCDKQDISDQLQNALGYIADGVCHPLKVRVETILQAEKDTIVLFTISNLLRFYQQIMRQVVQGGSLEECLVELQKSSEQIYLGALAAQVRSVLQRPSGGSGLALEPPQRDLVPPPSVARLLNMLKEILSVATMVDGRQADITKIVSCVIDPLLQSVQESAAHLPTVDMGVYLLNCLHHMQSSLAVYEYMDERVERLQAQSDAQLDTLTSEQASSLVANLNLGPIYAILQSNQSKIETNLLKIFMSKMDAFLELPDVLLLPQVQLIMSSSHRAAVQKRSFNVIVAIYKQIYERVHDPANGFEQPEQLLHRTPEQVAHIL"
  },
  {
    "reference": "MDAASVSFGSGHDLSSQPRHDWTRSEAETLYNLPFADLIFQAQTLHRRHFDPNHVETANLLSIKTGGCPEDCGYCSQSAHYDSGVKATKLMDREAVIETARRARDAGASRFCMAAAWRNPKDRDLDRVCDMVSAVKELGLETCATLGMLTPDQARRLHDAGLDFYNHNVDTSPEFYGNIITTRTMQDRIDTLAHAREAGLKVCCGGIIGLGEQVGDRLGMLMLLANLPAHPESVPINMWNEVKSVPVNDTAERPDPIALVRMIAVARIMMPRSVVRLSAGRQYMTDELQALCFLAGANSIFIGDVLLTTKNPQTARDAALLDRLGIRSRLDDAKAAAPPH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a fast turnover rate for (4R,5S)-dethiobiotin + [sulfur carrier]-SH + 2 reduced [2Fe-   2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'-deoxyadenosine +   [sulfur carrier]-H + biotin + 2 L-methionine + 2 oxidized [2Fe-2S]-   [ferredoxin].2. The protein design should aim to mimic or enhance the function of natural proteins involved in biotin biosynthetic process, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "MLCAICNVSSGNCAEDCAYCTQSAHIKADIPKFKQKNLEQILNEAKIASKNYALGFCLVTSGLGLDDKKLEFICEAATMLRKETPNLMLIACNGSASYESLKELKKVGIFSYNHNLETSREFFPQICTTHSWDDRFNTNLNAKKAGLELCCGGIYGLGESQDDRLSFRASLKELNPFSSPINFFIPNPALKIKQPLLSTDEALEIIRDTAKTLPQCY",
    "response#2": "LCPENCNYCSQALGSDAPILKYSWLSKDETLKQTGAGLRGGASRVCLVSSGRGPSTRDIDKVTEMVVALKEEYPGVEVCACLGLLKDGQAQRLKDAGVDAYNHNINTAESNHDNIVQTHTYADRVDTVEKAKGGGLSPCSGLIAGLGETDEQLVEAPVNLLTPVAGTPLERMDPLPPFEALRTIAVFRFMHPQADILVAGGREHVLGEYRSWTFISGANGLMVGNYLTTAGRSTADDYTMLRHMGVLP",
    "response#3": "LCAICNVSSGNCAEDCAYCTQSAHIKADIPKFKQKNLEQILNEAKIASKNYALGFCLVTSGLGLDDKKLEFICEAATMLRKETPNLMLIACNGSASYESLKELKKVGIFSYNHNLETSREFFPQICTTHSWDDRFNTNLNAKKAGLELCCGGIYGLGESQDDRLSFRASLKELNPFSSPINFFIPNPALKIKQPLLSTDEALEIIRDTAKTLPQCRIMVAGGREIVLGDRQYEILENGASAIVIGDYLTTSGEVASKDIEELRNRGFEFASQLCAICNVSSGNCAEDCAYCTQSAHIKADIPKFKQKNLEQILNEAKIASKNYALGFCLVTSGLGLDDKKLEFICEAATMLRKETPNLMLIACNGSASYESLKELKKVGIFSYNHNLETSREFFPQICTTHSWDDRFNTNLNAKKAGLELCCGGIYGLGESQDDRLSFRASLKELNPFSSPINFFIPNPALKIKQPLLSTDEALEIIRDTAKTLPQCRIMVAGGREIVLGDRQYEILENGASAIVIGDYLTTSGEVASKDIEELRNRGFEFASQL"
  },
  {
    "reference": "MLPVVTAKEMQKADNAAIERLHISETRLMELAGRECLRIIFEVLQTDSLRRSNFLIICGKGNNGGDGFVLARHLLNNGASVDLVLLYPESFLNGINREGFAILQAYEKHLDTLRIFTTLDEALPFIEKTNYNIVVDAITGTGLRLSEPEMALPSPLADGIDLLNTLHSETEAITLAIDIPSGLDATTGFSANPVIEADLTVTMAFLKRGFYLNDGPECCGEVHIAEISIPRFLVEPCSCLLVDHEFAAEHFTLRERNSAKHTNGKVLIIAGSQTEESSMLGAAILAAKAALKTGAGYVCVSLPFVLANAMHLAVPEAVVIGRDSNTLVEKIRWADVILIGCGLGRDQSAIELVRELLEYSGLSEKKLILDADALYALSSLGVSALSKCKNVLITPHYGEFSRLSNILIETIAAEPIQTARQFANDYDITVLLKGSPTVIVTKDAPVLLNTSGTEALATAGSGDVLSGIISALAAKGASLFNAAASAAWFHGRAGDLASDVASLVSSGMVIDALPLAIEEIFTREE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The K(+) binding site should be located in a region of the protein that is accessible to the ligand.2. The protein should have a fast turnover rate for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.3. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.4. The protein must be able to metal ion binding, NADPHX epimerase activity. The designed protein sequence is ",
    "response#1": "DNPDWKPVEPSGYLSLHKHARGRVAVFSGGSQSSGAGRLAAAGALRAGAGVVTLCTPPSASLVNAAHLTAIMLARWGSDDQIGEVLTGLRAEAAVLGPALGVGPATRKAVLGALEANVPLVLDADALTSFADDADSLVDQLHDRCVLTPHHGEFSRLFGEGRPNWNKLEQAQSAADRCGCTVLLKGPATIIATPGLTPWINRHACRWLATAGSGDVLAGIIAAGLAAGMSPHDAAAGATWLHGDAALRQGAGMTAEDLPDALPGALQAL",
    "response#2": "EAHKYSRGVVSLSAGEAMPGAARLAAASARRTGAGLVRIAAGKAAPAFRLGEPGLIIDEEPLADLLKDHRRRTWICGPGLLPEEVEQVFPQLVAEDRQILADAGAFSWAAGAPDRLRGAAVLTPHGGEFARVFGAPGSDPLAAVRAAAKQVNAVVVLKGATSVIASPDGKVALNTHATSALGTAGSGDTLTGIIGALLAAGMEPWDAACAGVWLHGEAALQAW",
    "response#3": "PERVLETLKPKLHENGLPLWVDHFNWPKDGGHKYDRGHAVVVSGPALQTGAARLGARAALRVGAGLVTLVGNAAATAIIATQITSIMVRSVSGATALSEFLRDTRRNAVLIGPGASVGPETADDVLAVLRSSASVVLDADALTSFSDTGEKPRSEASMGFLARNGDNPHGRETLFAAIKKRPAPVAMTPHEGEFKRLFGELPGSKIDRAREAAKLSGAFVILKGADTVIAAPDGRAAVNSNAPPWLATAGSGDVLGGLVTGLLAQHMPPFEAACAAVWLHGEAANAFGMGLIAEDLPDVLPKVLQRLFSEAGQADRLAAEAGVATISLMERAGQAVAEAALGMVWGPDRRVAVLCGPGNNGGDGFVAARHLRDKGWDVRLFLLGDRDALKGDAAEMARRWPLVVRPATPDALQSMHLVIDALFGAGLSRPLEGEAAELVRAVNASGLPVLSVDVPSGLDGTSGEVRGVAVQARRTVTFFRKKPGHVLMPGRQLCGEVIVADIGIPR"
  },
  {
    "reference": "SMASPPACAGPPALPSSPSTPGGCLATPELASLPPGSCAACQGVSFHIQIGLTREFVLLPAASDLAHVKQLACSIVDQKFPECGFYGLYDKILLFKHDLSSANILQLVRVAGDIQEGDLVEVVLSGLASCKRESACKPHHFQGLSLSLSQFSSLYFELERPGLRALAGCGALNYLEFCSICVSSKVRGGLARLAASPSFSSPSVCPHSSQSAASMASTTRSCSSNMTCRLPTSCSWCVASEIIASVRPSVLHLDRSLRRSVRPSVSYLDHSGGTRPTVCQFCKKLLKGLFRQGLQCKGSSSAVTGCNKRLGISLGKGLLRSYVPMEDVSDLSDADKNSLMDESDDSGIIPGSHSENELRGGDDPDDDASKSQGSMGYIPLMRVVQSVRHTNRKSSTALKEGWMVHYSSKDTLRRYQWKPPLPRRALSQNNPSSRYYKEIPLSEILAVELAQNFTPVLPGANLHCFEIVTANATYYVGENCAPAPCSASQQHSGVGLEVATAWENAIRQALMPVILQDAPSAQGQAPHRQASLRISVSNSQIEENVVSISSRSRSLCIERKREQTGQEEYKRITQNQRSPGHKTSYIPQATESPQSLRHAGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPQPSAPNCPSFSSAPLGGRGEPTSVRPSVRRAKLCLGRVPAAPGGGSVRFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIIWNSLSNPSPCNTPSPPPPSVPPQGPAAQCPLSPTARCP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should contain one or more Protein kinase domains that are essential for its function.2. The designed protein should have protein kinase C binding, calcium-dependent protein kinase C activity within cytosol to regulate positive regulation of cell adhesion, protein autophosphorylation, positive regulation of interleukin-8 production. The designed protein sequence is ",
    "response#1": "MAPFLRISFNSYELGSLQVEDETNQPFCAVKMKEALSTERGKTLVQKKPTMYPEWKSTFDAHIYEGRVIQIVLMRAAEEPMSEVTVGVSVLAERCKKNNGKAEFWH",
    "response#2": "FKFGKILGEGSFSTVVLARELATSREYAIKILEKRHIIKENKVPYVTRERDVMSRLDHPFFVKLYFTFQDDEKLYFGLSYAKNGELLKYIRKIGSFDETCTRFYTAEIVSALEYLHGKGIIHRDLKPENILLNEDMHIQITDFGTAKVLSPDSKQARANSFVGTAQYVSPELLTEKSACKSSDLWALGCIIYQLVAGLPPFRAGNEYLIFQYIHDLDNNSFELDLQFSEDEKRLLLEKQAGGNPWHQFVENNLILKMGPVDKRKGLFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKNFKTFF",
    "response#3": "IGKYLLLEPLEGDHVFRAVHLHSGEELVCKVFEISCYQESLAPCFCLSAHSNINQITEILLGETKAYVFFERSYGDMHSFVRTCKKLREEEAARLFYQIASAVAHCHDGGLVLRDLKLRKFIFKDEERTRVKLESLEDAYILRGDDDSLSDKHGCPAYVSPEILNTSGSYSGKAADVWSLGVMLYTMLVGRYPFHDIEPSSLFSKIRRGQFNIPETLSPKAKCLIRSILRREPSERLTSQEILDHPWL"
  },
  {
    "reference": "MRRQDFSYELPDELIARRPTETRTGSRLLMLDGPSGELAHKQFPDLLNLVEEGDLMVFNNTRVIPARVFGQKASGGKLEILAERILDAHSILAHVRSSKAPKPGSIIILQDGTEVEMVARHEALFELRFPADTTALDVLENIGHMPLPPYIDREDEADDKERYQTVYGTQKGAVAAPTAGLHFDDVLLAKLKDKGVQQAFVTLHVGAGTFQPVRVDNIFEHHMHSEVMHVSQEVCDLIKATKAAGKRVIAVGTTSVRCLETAAQNGVVEPYQGDTDIFIYPGYQYKVVDALVTNFHLPESTLLMLVSAFAGYKHTMNAYQQAVAQCYRFFSYGDAMFITHNAEACKEEIK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a specific function in queuosine biosynthetic process and should be localized to cytoplasm in order to carry out this function efficiently.2. The desired protein should be able to perform 7-aminomethyl-7-carbaguanosine(34) in tRNA + S-adenosyl-L-   methionine = adenine + epoxyqueuosine(34) in tRNA + H(+) + L-   methionine The designed protein sequence is ",
    "response#1": "MWLSLLFVPFLNAIPL",
    "response#2": "LTVNPL",
    "response#3": "MNTTFLILAAIVILFAIVGVIRR"
  },
  {
    "reference": "MALTQEQKMEIISKYQLHEKDTGSPEVQIAILTERINQLTEHLKVHKKDFHSRRGLLKMVGQRRSLLNYLKRKDFERYRAIVEKLGLRK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design must be focused on enhancing the efficiency of translation. The designed protein sequence is ",
    "response#1": "YDCYANLALLKLYQFNPHLSRDETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAKGTVVREC",
    "response#2": "DETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAC",
    "response#3": "DETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAC"
  },
  {
    "reference": "MTVEGGVQYSRIAEIKGPLVIVDGVENAAFDELVEIETNEGGRRLGKVLEIGNGKAIVQVFEGTTGLSVAGTNARFVGKVMEMPVSREVLGRIFDGLGRPKDKLPDPIADKFIDINGEAMNPEQREYPKDFIQTGVSAIDGIMTLVRGQKLPIFSGSGMSHNILAAQIARQASVVGTGDDFAVVFAAIGVQYSEAEYFRRSLEESGALKRSVLFLNLANDPAIERIITPRVALTVAEYLAFDLGMHVLVILTDMTNYAEALREISAAREEVPGRKGYPGYLYTDLSTIYERAGRLVGRKGSVTQVPILTMPSDDITHPIPDLTGYITEGQVVLGRDLFRQGVYPPVNILMSLSRLMKDGIGEGRTREDHQEISNQNYDAYSRAQEVRALAGIVGKAGLTDIDLRYMGVGDSLEQRLLTQATDENRTIEETLGIMWDTVSGLPKNELTKVKDKYVEKFYKGSA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that The archaeal beta chain is a regulatory subunit.; Produces ATP from ADP in the presence of a proton gradient across the membrane2. The designed protein must be able to perform proton-transporting ATP synthase activity, rotational mechanism under specific conditions. The designed protein sequence is ",
    "response#1": "GQRLEDTFFGLAKGGASAIPGPFGSGKTVTQHQLAKWSDADIVVYIGCGERGNEMTEVIEEFPHLDDLKTGNKLMDRTVLIANTSNMPVAAREASVYTGITIAEYFRDMGYGVLLTADSTSRWAEAMREISGRLEEMPGEEGYPAYLSSRLAQFYERAGRVACMGHDEKQGFVCIVGAVSPPGGDFSEPVTSNTLRIVKVFWALDANLARRRHFPAINWLQSYI",
    "response#2": "GQRLEDTFFGLAKGGASAIPGPFGSGKTVTQHQLAKWSDADIVVYIGCGERGNEMTEVIEEFPHLDDLKTGNKLMDRTVLIANTSNMPVAAREASVYTGITIAEYFRDMGYGVLLTADSTSRWAEAMREISGRLEEMPGEEGYPAYLSSRLAQFYERAGRVACMGHDEKQGFVCIVGAVSPPGGDFSEPVTSNTLRIVKVFWALDANLARRRHFPAINWLQSYV",
    "response#3": "MKKVAGTLKIDQAQYRELEAFTKFGGDMDAVTRATLDKGRKNVELLIQSQYNPLPVEKEIAIIFIGIKGLLSDVPVERVREFETEFLDILEMKYRKDILDMLKQGVLDEKIEKKLNTIASIVANNL"
  },
  {
    "reference": "MAKSNYITREGWQALDRELHYLWREERPVVTQAVSEAAAMGDRSENAEYIYGKKRLREIDRRVRFLAKRLEVLKIVDPDPRQEGKVFFGAWVRVENEQEEQRIFRLVGPDEFDPAKKWISIDSPVARALIGKQVDDEVTVQTPNGEATYWILEIRYRPFDEDRPFNEDIDN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit DNA binding.2. For general function, the protein need meet that Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus; Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites; GreB releases sequences of up to 9 nucleotides in length. The designed protein sequence is ",
    "response#1": "MW",
    "response#2": "GNYITREGWQALDRELHYLWREERPVVTQAVSEAAAMGDRSENAEYIYGKKRLREIDRRVRFLAKRLEVLKG",
    "response#3": "SNYITREGWQALDRELHYLWREERPVVTQAVSEAAAMGDRSENAEYIYGKKRLREIDRRVRFLAKRLEVLKN"
  },
  {
    "reference": "MYIYRKPYEQNYWNNFILLSLYVNNKRVGVNKNITKLEYILLDIFLHGPLHTEFINYLEITNYINNRTYLYEKMLKEKSVNAINIILPPVMHTASFEYNYEIIEDLDSNKILNIYILNNKCYYCRKLKDDSYWEKFPFNEIPIFVNDKKVGLRLKTDKLKTTKNLTKVEYYLLDIFWFGPLKIEASEHYEKIMRQIKDRNKKYMCLYYEKIINGINIIFNISNNIEYKKFLNNDYSIKEMINVEHILTIYVLT",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be expressed specifically in the plastid The designed protein sequence is ",
    "response#1": "IKPYNDDPFVGHLATPITSSAVTRAILQNLPAYRFGLTPLLRGLEIGLAHGYFLMGPFVKLGPLRDSEIGLLAGFLSTVGLIVILTLGLTIYGVAAFGQEKTQSSNENDLQTKKAWDQFKGGFFVGACGSAGFAFICLSSR",
    "response#2": "IGGLVAATQLAVKGAKVLVLEKYLIPGGSSGYYERDGFTFDVGSSVMFGFSDKGNLNLITQALAAVGCKMEVIPDPSTVHFHLPSNLSVLVHREYNEFFSELTSKFPHEKEGIFKFYGECWKIFNALNSLELKSLEEPIYLFGQFFKKPMECLTLAYYLPQNAGDIARKFIKDPEVLSFIDAECFIVSTVNALKTPMINASMVLCDRHYGGINYPVGGVGGIAKSLAKGLVDQGSEIQYKANVKSIIVENGKAVGVRLANGNEIFAKNIISNATRWDTFGKLLKQDELPKEEENFQKLYIKAPSFLSIHLGVKSDVLPPDTDCHHFVLEDDWSNLEVPYGSIFLSIPTVLDSSLAPEGNHILHIFTTSSIEDWQGMSQKDYEKKKELVADEIISRLEKKLFPGLQSSIVLKEVGTPKTHRRYLARDSGTYGPMPQGTPKGLLGMPFNTTAIDGLYCVGDSCFPGQGVIAVAFSGVMCAHRRLAG",
    "response#3": "IKPYNDDPFVGHLATPITSSAVTRAILQNLPAYRFGLTPLLRGLEIGLAHGYFLMGPFVKLGPLRDSEIGLLAGFLSTVGLIVILTLGLTIYGVAAFGQEKTQSSNENDLQTKKAWDQFKGGFFVGACGSAGFAFICLSSA"
  },
  {
    "reference": "MNALHCVVLAAGQGTRMKSALPKVLQPIGGQPMLAHVIDTARALLATTHVVYGHGGEQVQAFFAEQTQLHWAHQAEQKGTAHAVQQAMPQIPDDATVLILYGDVPLIRAATLQSLLAQVGGGLAVLTVQLSNPTGYGRIVRNAGRQVQGIVEEKDANAAQKAITEVNTGVMAASAQRLRAWLSRIGNANANGEFYLTDVVALAVADGVTVNTVSASSAEEVEGANDRIQLARLERAYQRREVENLMHQGVRMADPARVDIRGRVMAGRDVFLDVGCILEGEVTLGDNVHIGAYSMVRDSEIGADTQIEAHSILDRARVGKACQIGPFARLRPQAQIGDEAHVGNFVEIKKTTLGARSKANHLAYLGDGEVGSDVNIGAGTIFCNYDGANKHLTVIEDGAFIGSDSQLVAPVRVGRGATVGAGSTIAKDVPAGGLTICRAREQKTFANWQRPRKTPKA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be designed to have high stability and activity towards acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.2. A protein should be able to manipulate the activity of enzymes in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway, either by activating or inhibiting their function.3. The designed protein must have at least one MobA-like NTP transferase domain.4. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).5. A protein with magnesium ion binding is needed to enhance regulation of cell shape in a specific context. The designed protein sequence is ",
    "response#1": "VVILAAGMGKRMQSALPKVLHPLAGKPLLAHVLDTARSLATNRVCVIYGHGGAAVLELLGKQPQPVATAQQEQQLGTGHAVMQALPELDDNAPTLILYGDVPLTTAASLQALIDAAGTDKLGILTVVQDPAGLGRIVRNAQGALERMVEHKDASEAELAIREINSGILAAPTSHLKQWLGNLSNDNAQSEYYLTDIVALAVQDGLPVVAAQPETEVEVLGVNSKVQLAELERWYQREQARRLMQQGASLADASRIDVRGSLNTGNDVFIDINCVFEGEVTIGEGVRIGPNCHISDSKIADNAEIKANSVLEGAVVGEACSIGPFARLRPGTVLAADVKIGNFVETKKANIGLGSKVSHLSYIGDAEIGRDVNVGAGTITCNYDGVNKFKTTIADDVFVGSNTALVAPVSVGKAATIGAGSVITGNIAEGELAVARSKQRNIQGWQRPVKIKK",
    "response#2": "ILLAAGLGTRMNSDMPKVLHQVAGAPLLIHAMKSAAALTPERTIVVAGHGADAVEAAAKDWDEDVRVALQTEQLGTGHAVLQAKEALAGHTGDAIVLYGDTPFIRPETLEAMIEARQSHAVVVEAADPDGLGRVVRTGERVVRIVEHRDATASELAVREINTGLMAVRAEDLRRWLGRLTNDNAQGEYYLTDIVAMAAADGRVVAAVHPDEVWEVEGVNNRQQLAALERRHQLNLARRLMAEGASLADASRIDVRGSLNTGNDVFIDINCVFEGEVTIGEGVRIGPNCHISDSKIADNAEIKANSVLEGAVVGEACSIGPFARLRPGTVLAADVKIGNFVETKKANIGLGSKVSHLSYIGDAEIGRDVNVGAGTITCNYDGVNKFKTTIADDVFVGSNTALVAPVSVGKAATIGAGSVITGNIAEGELAVARSKQRNIEGWQRPVKK",
    "response#3": "LILAAGQGMRMESSIPKVLHHVGGRPMLFYILRIANALKPGAIGVVVGHESDQVKAATLEMARSVGITRAICFITQKKPLGSGNAVVESLPFLKKFKTAAVMCGDTPLLTYESIYNLLLTHDQLKGQATLLTARIANPKGYGRIIRGALGEVQRVVEESVATSKEAAVNEVNSGAYVFELEALISGLKKIGAKGPKKEHFLTDVMEAIRAKGKIGGIICDASELMEDGVTMTAPETVFLAHDTVIGRETIVEPNVVFGPGVTVESGARIRAFSHLEGCHVSQGAEVGPYARLRPGAELSERAKVGNFVEVKNATLAEGAKVNHLTYIGDANVGARSNIGAGTITCNYDGVFKHHTEIGADAFIGSNTMLVAPVTVGAGAMTASGSVITRDVPDGAMGIGRGRRLIQGYVYPGVRLADPARLDVRGQLRCGRDVAIDVGCVFEGEVVLEEAVDIGPYCVLKNVTVKAGARIAAFSHLEGAVVGPDAIVGPYARLRPGTELGAEAHVGNFVELKNSILGPGSKANHLAYVGDATVGQRVNIGAGTITCNYDGANKHRTVIEDDAFIGSDSQLVAPVTVGRGATLGAGTTLTKDAPADSLTVSRARVVENVPGYLRR"
  },
  {
    "reference": "MSDITLILLCAGNSSRFELSSKKQWLRTENSPLWLFVTKRLSSFYSFNKIIIASHKDELNYMKNFTDDVTFVEGGETRQSSMKNALKEVTTDYVMVSDVARACIPKEVITNLIDAKNNADCIVPILNVSDTVVFKNDTINRDEVKLIQTPQLSRTEILINALNTDEEFTDDSSAIKNIGGTIEYIKGSLNSKKLTFEEDIQAIECIKAPSKNFFTGTGFDIHPFEDNKKMFLGGVNIDVDYGFKAHSDGDVLIHSVIDALLGACGAGDIGEFFPDTDERYKGADSKELLKHIVKFIYNVGYEIVNIDLTIIAQKPKINPYKNEIKSTMAQLLNLEKQFVNIKATTAEKLGFIGRSEGVAVQSIATLKYYEWERK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The a divalent metal cation binding site should be stable and able to withstand changes in environmental conditions.2. For general function, the protein need meet that Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D- erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C- methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).3. The protein should be designed to catalyze the following reaction 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-   erythritol 2,4-cyclic diphosphate + CMP.4. A protein should be able to manipulate the activity of enzymes in the isopentenyl diphosphate biosynthesis via DXP pathway pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "SADNLKITRPEDFARAARILETRMDIRTGNGFDVHAFGPGDHVTLCGIDIPHTYGLVGHSDADVALHTVTDAIYGALARGDIGQHFPPSDPQWKGARSDIFLRHAAQMARDMGFTLTHVDCTLVCETPKIGPHVEPMRAAMAEMLGIEIDRVSVKATTSERLGFTGRGEGIACMATATLVLVDDVAAGRTDDAAVAEWAGHRVHVFAGEDANMKVTTCKDLAIAEARLLGDLADVRTGQGYDVHAFGPGAHVWLGGLKIAHNHGLIGHSDADVLTHAITDALLGALADGDIGSHFPPSDPQWRGAASSIFLAAAAAKVRSRGGIIAHIDATLVCERPKLGPHRDAIRASIARIAEIPLDRVAIKATTSEGLGFTGREEGIASFAVATVRIGTGFDVHRLAAGEELWLGGIKIESDYGLAGHSDADVAIHAIVDALLGAIANGDIGSHFPPSDPQWKGASSDRFLKHAALLVNNSGFRIGNVDLTIICEAPKIGPHREAIRVRLAELLGVGIPTISVKATTTEKLGFAGRGEGIAAQAVATVVVS",
    "response#2": "TDDAAVAEWAGHRVHVFAGEDANMKVTTCKDLAIAEARLLGDLADVRTGQGYDVHAFGPGAHVWLGGLKIAHNHGLIGHSDADVLTHAITDALLGALADGDIGSHFPPSDPQWRGAASSIFLAAAAAKVRSRGGIIAHIDATLVCERPKLGPHRDAIRASIARIAEIPLDRVAIKATTSEGLGFTGREEGIASFAVATVATVAEPLVAPGLDVHRLEDGEELWLGGVLIPHTQGLSGHSDADVALHAITDALLGTIAAGDIGQHFPPSDPQWKGAASDQFLAHAATLVRDRGGQIGFIDLTLMCEAPKIGPHREAMRRRIAAILNIAEERVSVKATTTEKLGFTGRGEGIAAQAIATVVRE",
    "response#3": "SADNLKITRPEDFARAARILETRMDIRTGNGFDVHAFGPGDHVTLCGIDIPHTYGLVGHSDADVALHTVTDAIYGALARGDIGQHFPPSDPQWKGARSDIFLRHAAQMARDMGFTLTHVDCTLVCETPKIGPHVEPMRAAMAEMLGIEIDRVSVKATTSERLGFTGRGEGIACMATATLVLS"
  },
  {
    "reference": "MFQNNPLLAQLKQQIEASKEYVEGVVKTSDKSYGFLECEKNSYFIPPAEMKKVMHGDKVKAVVKRDGDKEQVEIDSLLEPMLERFIAQVRFNKDGKLQLAVDHPSINNFIPANTQKKVTEPLENGDWVVAQLKTHPLRDDRFFFAQVTQFICKADDNFAPWWVTLARHEQPREPVANEKSYELQDQVEREDLTHLYFTTIDSASTKDMDDALYVEPISENGTQTGWRLVVAIADPTAYIPEQSAIEKAARQRCFTNYLPGFNIPMLPRELSDDLCSLVPNEKRPALVGYIETDLSGNVVGEARFVSAWVQSKARLVYDEVSDYLEKVENHWTPDCAETAQQIDWLHQFTLARIDWRSKNALLFKEQGDYSFELAEDGAVKAIHIDYRRIANQMIEEAMIIANICAAQFLDKYAHTGVFNTHSGFDSKNLEPARKFLLDTLANDENRESLSERYSPERLSTLEGYCEMRRDIEQFPENFLEMRLRRYLTFAEFKATSAPHLGLGISHYATWTSPIRKYGDMVNHRLIKQVLSNQTAKPVEETVLARLQEARKQNRMVERDIADWLYARYLEPMVEQNVEFDGEIQDVSRGGLRVKVIENGASVFVPFSTLHNNKEEMLFSPEEIALYIKGEKAYQIGQAVKVKLKEVRVETRSVVGDVLI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein localizes to the cytosol.2. The protein must exhibit the following characteristics: Hydrolyzes single-stranded polyribonucleotides processively in the 3' to 5' direction..3. The designed protein have S1 motif domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "ELESISETIYRVDTNKAQKEDIVLNSQNRISPSETGNQVDRSPEPLFTYVNEKLFSKPTYAAFINLLNNYQRATGHGEHFSAQQLEEQGVFLREVMKTAVMKELYSFLHHQNRYSSEQEFVDDLKNMWFGLYSRGNDEGDSSGFEHVFSGEVKKGKVTGFHNWIRFYLQEKEGLLDYYSHNYDGPWDSYPDVLAMQFNWDGYYKEVGSVFIGSSPEFEFALYSLCFITRPGKKCHLSLGGYPLAIQTYTWDKTTYGNGKKYIATAYR",
    "response#2": "ELESISETIYRVDTNKAQKEDIVLNSQNRISPSETGNQVDRSPEPLFTYVNEKLFSKPTYAAFINLLNNYQRATGHGEHFSAQQLEEQGVFLREVMKTAVMKELYSFLHHQNRYSSEQEFVDDLKNMWFGLYSRGNDEGDSSGFEHVFSGEVKKGKVTGFHNWIRFYLQEKEGLLDYYSHNYDGPWDSYPDVLAMQFNWDGYYKEVGSVFIGSSPEFEFALYSLCFITRPGKKCHLSLGGYPLAIQTYTWDKTTYGNGKKYIATAYY",
    "response#3": "RKDLTDLDFITIDNTNTKDIDDALFVSEKNNGDISLIVAIADPTAYIKHGSKLDVIASKRIFTNYLPGFNIPMLPRNLSEDICSLNPNKRRPVLACHITVLKNGNISNKIEFFLAWIKSKSKLSYDHVSDWIEKIGSWIPPTKSIANQILILHRLCLLRIKWRKKNAVLFKDSIEYRFHVSEHGKIIDVLIEKRRIAHKIIEESMIVANISAANFLSKNIGFGIYNVHSGFDLVNAENAVSFLRSYNLNFTVKEITTLKGFCNLRRVLNIISNNYIDSRIRRFQSFGDFSTIPGPHFALGFSEYATWTSPIRKYSDMINHRLLKSIID"
  },
  {
    "reference": "MAASPNPLQGSLFEESKQSTTNGGKETNNSIGSSENLSNQQLKSDASLRPRIRKTSKNPNQINDLDQLSNAEIEEPKWSHHNLPKIDDLTPALKHYVQLKIENPDRVLLYRLGDFFECFFEDAITLSQLLEITLTSKEGGKKIGKVPMAGIPHHASDRYCTELIKKGLSIAICDQLEAAPSKGNKLIKRGITRLITPGTIIEEGMLSAKQNNWLASVLLESDSNSDFVNWSLAKLDVSTGEFLVQEGKETNNLRQELIKLKAAEVISESKSISNQNWYKGLIEITEFNQTSFSRLEAKTTIENHYFLNNIDGLGIHPESLSIRTIGGLIAYLNKTHPNIGNNLKNEVKTNICIDFPQIKHNQAGLIIDNQTRRNLEITSTQKNGQFQGSLLWAIDKTLTAMGGRCIRRWLEEPLTEIYSIQSRQKIIGLLVESSSLRKNIRKILRAMGDLERLSGRAGAQQAGARDLIAIAEGINRLPLIKKYLNDPIFEETKYFESIINLDRDLIELASKINNEIIDNPPLSLTEGGLIFDGVNPILDGLRNQLDDHNSWLKSQEIEERKNSNINNLKLQYHRSFGYFLAVSKAKSINVPDHWIRRQTLTNEERFVTPELKEREGKIFQLRARISQLEYDLFCKLRILVGNKSDIIRKAAKAISCLDVLSGLAELAATNNYIQPKIIDNKDSTKTRRLSIVDGRHPVVEQILVDKFFVPNDIELGSKTDLIILSGPNASGKSCYLRQVGLLQIMAQIGSWIPAKSANIGIADQLFTR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: This protein has a weak ATPase activity..2. The ATP binding site should be stable and able to withstand changes in environmental conditions.3. The protein should be able to modulate mismatch repair in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "MITGPNMAGKSSYLRQTGLIVLLAQAGCFVPAEQAEIGVVDRIFTRVGASDNLASGESTFLVEMNEAADILNNATAKSLLLLDEIGRGTSTYDGLAIAWSMCEYIHRQIGARTLFATHYHELAELEGLLPGVVNYNASVLESGDRQ",
    "response#2": "MITGPNMAGKSSYLRQTGLIVLLAQAGCFVPAEQAEIGVVDRIFTRVGASDNLASGESTFLVEMNEAADILNNATAKSLLLLDEIGRGTSTYDGLAIAWSMCEYIHRQIGARTLFATHYHELAELEGLLPGVVNYNASVLESGDRR",
    "response#3": "IITGPNMAGKSTYMRQTALIVLMAQIGSFVPAATAKIGLVDRIFTRVGASDDLASGQSTFMVEMSEVANILINATKRSLLVLDEIGRGTSTFDGLSIAWAVIEYIVNKEQLGCRTLFATHYHELTELEGKLPGIKNYCITVKEKGEDVIFK"
  },
  {
    "reference": "MATPHINAQVGDFAETVLMPGDPLRAKFIAENFLEDVTQVCDVRNMFGYTGTYKGKKVSVMGHGMGIPSCCIYVHELIAEFGVKNIIRVGSCGAVHDDVKLMDVVIGMGASTDSKVNRIRFNNHDFAAIADFGLLETAVAQARKLNVPVKVGNVFSADLFYSPETDLFDKMEKLGILGVDMEAAGIYRVAADLGAKALTILTVSDHIKRGEKLSSEDRQKSFNDMMTVALETAIKL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be able to bind phosphate, a purine D-ribonucleoside ligand in a variety of conditions2. The protein with the reaction a purine 2'-deoxy-D-ribonucleoside + phosphate = 2-deoxy-   alpha-D-ribose 1-phosphate + a purine nucleobase, the Proton donor must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "VLMPGDPLRAKYIAETYLENAELVTDVRNMFGYTGFYKGQRVSVMGHGMGIPSCSIYTKELITEYGVKNIIRIGSCGAVRPDVKVRDVIIGMGACTDSKVNRMRFKDHDFAAIADFGLLRKAVTAAETLGIPVKVGNLFSADLFYSPDPQMFDVMEKFGILGVEMEAAGIYGVAAEYGANALAICTVSDHIKTGEQTSADERQNTFDA",
    "response#2": "VLMPGDPLRAKWAAERFLENPVCVNEVRGMLGFTGMWRGNRVTIHGSGMGMPSLSIYANELLRDYGAKTLIRIGSCGAMQADVKLRDVILAMTASTLSTPSSGIFRELNYAPCADYGLLQAAYKAAEGKGSPIHVGGIYSSDVFYDERPDLNEQMVRHGVLAVEMEAAELYTLAARHKARALAVLTVSDHLLTEEALPSEER",
    "response#3": "VLMPGDPLRAKWAAERFLENPVCVNEVRGMLGFTGMWRGNRVTIHGSGMGMPSLSIYANELLRDYGAKTLIRIGSCGAMQADVKLRDVILAMTASTLSTPSSGIFRELNYAPCADYGLLQAAYKAAEGKGSPIHVGGIYSSDVFYDERPDLNEQMVRHGVLAVEMEAAELYTLAARHKARALAVLTVSDHLLTEEALPSEEQ"
  },
  {
    "reference": "MRLDRFLANLPELSRRDAQMLIAGGHLRVDGQVVRDGTHEVRVFSRVELDERLLQAGKPARYYMLHKPMGCVSATRDPQHPTVLDLFPEDLRDDLHIGGRLDYNTTGLMLLTNDGQWSRRLTLPGSRRDKVYYVETEAPIDQRYVDAFAAGLHFRFEDLVTLPAQLEPIGPRCARLTLHEGRYHQVKRMFGHFDNKVLRLHRERMGDICLDPQLPPGAWRPLNAEEICSV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that can perform RNA binding, rRNA pseudouridine synthase activity, pseudouridine synthase activity in cytosol to support enzyme-directed rRNA pseudouridine synthesis.2. For general function, the protein need meet that Responsible for synthesis of pseudouridine from uracil-516 in 16S ribosomal RNA.3. A key design requirement for the protein is the optimization of the Nucleophile to achieve high catalytic efficiency for uridine(516) in 16S rRNA = pseudouridine(516) in 16S rRNA. The designed protein sequence is ",
    "response#1": "MILVINKASNMVVHPGIGHYQGTVLNALLYRYPSILKISKQAGIVQRLDKDTTGLMIIAKTVSAYDNLLRSFKLRKVVKEYDAIVYGKFTSNAGVVNQPMRRHFAKRTFMSVHYTGKSAVTYYSVVEEFKAHSRVRINLKTGRTHQIRVHMAYINHPLVGDQKYKGNFSVFDIKKMSDELNNYLLDFNRQALHACTLQR",
    "response#2": "LAVVDKPAGVYCDALLSALPCSAATLGDEATKPNLHLANRLDRDTSGLMVITKCNKVAGKLVKAFTEHKVKKTYLALCIGYPPAWEKIKICSGHGRSKHGAWRVYAMSDVGRSLPGGSVVRDMSTRFEVLGINGKGQFREPSNFEVDETESITVQEKAADLTSDGDEKNSIILVRAYPQSGRTHQIRLHCQYLGFPIRGDVKYSGVIEWNGVDYDGHALHAESLSA",
    "response#3": "LAVVDKPAGVYCDALLSALPCSAATLGDEATKPNLHLANRLDRDTSGLMVITKCNKVAGKLVKAFTEHKVKKTYLALCIGYPPAWEKIKICSGHGRSKHGAWRVYAMSDVGRSLPGGSVVRDMSTRFEVLGINGKGQFREPSNFEVDETESITVQEKAADLTSDGDEKNSIILVRAYPQSGRTHQIRLHCQYLGFPIRGDVKYSGVIEWNGVDYDGHALHAESLSV"
  },
  {
    "reference": "MAKEKPILNVAFIGHVDAGKSTTVGRLLLDGGAIDPQLIVRLKKEAEEKGKAGFEFAYVMDGLKEERERGVTIDVAHKKFPTAKYEVTIVDCPGHRDFIKNMITGASQADAAILVVNVDDAKSGIQPQTREHVFLSRTLGISQLAVAINKMDTVNFSEADYNEMKKMLGDQLLKMVGFNPDNITFVPVASLHGDNVFKKSDKTPWYNGPTLAEVIDAFQPPEKPTTLPLRLPIQDVYSITGVGTVPVGRVETGIIKPGDKVIFEPAGAVGEIKTVEMHHEQLPSAEPGDNIGFNVRGVGKKDIKRGDVLGHTTNPPTVAADFTAQIVVLQHPSVMTVGYTPVFHAHTAQIACTFMELQKKLNPATGEVLEENPDFLKAGDAAIVKLMPTKPLVMESVKEIPQLGRFAIRDMGMTVAAGMAIQVTAKNK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The presence of tr-type G domains is critical for the interaction of the designed protein with its intended targets.2. The protein can be targeted to cytoplasm and perform GTP binding, GTPase activity, translation elongation factor activity.3. The protein must exhibit the following characteristics: This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis..4. The protein should be able to bind GTP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "SRIRNFSIIAHIDHGKSTLADRLIQFTGGLTEREMSAQVLDNMDIEKERGITIKAQTVRLSYTAKDGETYQLNLMDTPGHVDFAYEVSRSLAACEGALLVVDAAQGVEAQTLANVYQSIEHDHEIVPVINKIDLPAADVDKVKAEIEDIIGLPADDAVLASAKAGIGIEETLEAIVARIPPPKE",
    "response#2": "NFSIIAHIDHGKSTLSDRLIQVCGGLTDREMAEQVLDSMDLERERGITIKAQSVTLDYTAKNGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALEMDLEVVPVLNKIDLPQAEPERVAAEIEDIVGIEATDAVRCSAKTGVGIGEVLETG",
    "response#3": "RIRNFSIIAHIDHGKSTLADRLIQFTGGLTEREMSAQVLDNMDIEKERGITIKAQTVRLSYTAKDGETYQLNLMDTPGHVDFAYEVSRSLAACEGALLVVDAAQGVEAQTLANVYQSIEHDHEIVPVINKIDLPAADVDKVKAEIEDIIGLPADDAVLASAKAGIGIEETLEAIVARIPAPDRPVATP"
  },
  {
    "reference": "MRSSTAAVQRPAAGDPEPRRPAGWAARRSLPRTARRGGRGGAVAYPSAGPPPRGPGAPPRGPRSPPCASDCFGSNGHGASRPGSRRLLGVCGPPRPFVVVLLALAPAATPARACPPGVRASPPRSGVSSSARPAPGCPRPACEPVYGPLTMSLKPQPQPPAPATGRKPGGGLLSSPGAAPASAAVTSASVVPAPAAPVASSSAAAGGGRPGLGRGRNSSKGLPQPTISFDGIYANVRMVHILTSVVGSKCEVQVKNGGIYEGVFKTYSPKCDLVLDAAHEKSTESSSGPKREEIMESVLFKCSDFVVVQFKDTDSSYARRDAFTDSALSAKVNGEHKEKDLEPWDAGELTASEELELENDVSNGWDPNDMFRYNEENYGVVSTYDSSLSSYTVPLERDNSEEFLKREARANQLAEEIESSAQYKARVALENDDRSEEEKYTAVQRNCSDREGHGPNTRDNKYIPPGQRNREVLSWGSGRQSSPRMGQPGPGSMPSRAASHTSDFNPNAGSDQRVVNGGVPWPSPCPSHSSRPPSRYQSGPNSLPPRAATHTRPPSRPPSRPSRPPSHPSAHGSPAPVSTMPKRMSSEGPPRMSPKAQRHPRNHRVSAGRGSMSSGLEFVSHNPPSEAAAPPVARTSPAGGTWSSVVSGVPRLSPKTHRPRSPRQSSIGNSPSGPVLASPQAGIIPAEAVSMPVPAASPTPASPASNRALTPSIEAKDSRLQDQRQNSPAGSKENVKASETSPSFSKADNKGMSPVVSEHRKQIDDLKK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The ideal protein would be one that plays a critical role in cerebellar Purkinje cell differentiation, neuron projection morphogenesis through RNA binding within cytoplasm, trans-Golgi network, Golgi apparatus.2. The presence of Sm domains is critical for the interaction of the designed protein with its intended targets.3. The protein must exhibit the following characteristics: Involved in EGFR trafficking, acting as negative regulator of endocytic EGFR internalization at the plasma membrane.. The designed protein sequence is ",
    "response#1": "LSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKASKEQEGEYYCTAFNRANHASSVPRSKILTVRVC",
    "response#2": "QLRGEVWALLLEIPKMKEETRDLYSKLKHRARGCSPDIRQIDLDVNRTFRDHIMFRDRYGVKQQSLFHVLAAYSIYNTEVGYCQGMSQITALLLMYMNEEDAFWALVKLFSGPKHAMHGFFVQGFPKLLRFQEHHEKILNKFLSKLKQHLDSQEIYTSFYTMKWFFQCFLDRTPFTLNLRIWDIYIFEGERVLTAMSYTILKLHKKHLA",
    "response#3": "MTSISEDSDKEKARSVSVDLNNDASLLIDIPDALCERDKVKFTVHTKTTLSPFQKPDFSVPRQHEDFIWLHDAIVETEEYAGLIIPPAPPKPDFEGPREKMHKLGEGESSMTKEEYAKMKQELEAEYLAVFKKTVQVHEVFLQRLSSHPSFSKDRNFHIFLEYDQDC"
  },
  {
    "reference": "MIDNAYHIDSGYHYLVCNMDKQLNKAPTMNDEPAAKPPAGNKPLSVSSEQLLGEHGVAFIIHQGECYQLRQTKAGKLILTK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design must be focused on enhancing the efficiency of iron ion homeostasis. The designed protein sequence is ",
    "response#1": "VKWCALSHHERLKCDEWSVTSGGLIECESAETPEDCIAKIMNGEADAMSLDGGYVYIAGQCGLVPVLAENYESTDCKKAPEEGYLSVAVVKKSNPDINWNNLEGKKSCHTAVDRTAGWNIPMGLLYNRINHCRFDEFFRQGCAPGSQKNSSLCELCVGPSVCAPNNREGYYGYTGAFRCLVEKGDVAFVKSQTVLQNTGGRNSEPWAKDLKEEDFELLCLDGTRKPVSEAHNCHLAKAPNHAVVSRKDKAACVKQKLLDLQVEYGNTVADCSSKFCMFHSKTKDLLFRDDTKCLVDLRGKNTYEKYLGADYIKAVSNLRR",
    "response#2": "VKWCALSHHERLKCDEWSVTSGGLIECESAETPEDCIAKIMNGEADAMSLDGGYVYIAGQCGLVPVLAENYESTDCKKAPEEGYLSVAVVKKSNPDINWNNLEGKKSCHTAVDRTAGWNIPMGLLYNRINHCRFDEFFRQGCAPGSQKNSSLCELCVGPSVCAPNNREGYYGYTGAFRCLVEKGDVAFVKSQTVLQNTGGRNSEPWAKDLKEEDFELLCLDGTRKPVSEAHNCHLAKAPNHAVVSRKDKAACVKQKLLDLQVEYGNTVADCSSKFCMFHSKTKDLLFRDDTKCLVDLRGKNTYEKYLGADYIKAVSNLRR",
    "response#3": "VKWCALSHHERLKCDEWSVTSGGLIECESAETPEDCIAKIMNGEADAMSLDGGYVYIAGQCGLVPVLAENYESTDCKKAPEEGYLSVAVVKKSNPDINWNNLEGKKSCHTAVDRTAGWNIPMGLLYNRINHCRFDEFFRQGCAPGSQKNSSLCELCVGPSVCAPNNREGYYGYTGAFRCLVEKGDVAFVKSQTVLQNTGGRNSEPWAKDLKEEDFELLCLDGTRKPVSEAHNCHLAKAPNHAVVSRKDKAACVKQKLLDLQVEYGNTVADCSSKFCMFHSKTKDLLFRDDTKCLVDLRGKNTYEKYLGADYIKAVSNLRKCSTE"
  },
  {
    "reference": "MIDFKDLLSRLGRQRVLCVGDLIIDEFVYGKVSRISPEAATPVLAAQRSELHIGGAGNVARNITSLGARCDFVGVIGCEPDAYKIASEHLQAESVDAHLVTDASRPTTRKIRFVSEHYSTHLLRTDWESAEAIGGGTEEAVLERATAALPRAGALLLSDYAKGVLTKRVVRTLITAAQQAGLPIVVDPKGHDYSVYRGATLITPNRQELAQVTRLPVGNDAEIEQAAARLSELTDSAAVVVTRSEDGMSLFRRGEGALHISAHPVRVRDVAGAGDTVVATLATFLAAGADYEPALRAANAAASVAVGKRGTATVSVAELRARLVPAAARANEEKILVDHGLLSGHLAQWRRDGMRIGFTNGCFDLLHPGHIRLLVEARAACDKLIVGLNSDASVARLKGRDRPVQNEQARAEVLAALEAVDLVVIFDEPTPLELIRTVRPAVLVKGSDYRPEEVVGRDLVEAYGGEVMLVEIVPGHSTTNLVRRMRSPHEEVIEQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit phosphotransferase activity, alcohol group as acceptor.2. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. The designed protein have Cytidyltransferase-like domains with specific sequence motifs that are crucial for its activity.4. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7- phosphate at the C-1 position to selectively form D-glycero-beta-D- manno-heptose-1,7-bisphosphate.. The designed protein sequence is ",
    "response#1": "HGRGEMLAMGGAGNVVSNIVSLGGAAIPVSVIGADQAGDNLMRMLGELGADTDGLTQRQDRMTSSKSRFSALNQQVLRFDEEEIKPLSRGERATLVEHFQAALARADIVILSDYGKGILLDGVAADLIAICRDAGKPVLVDPKGRDYARYAGATAVTPNRKELGEAVGRAVFGDDEITAAARDLIAVHDFEFIVATRSEKGMSVVTAEDARHISTQAREVFDVSGAGDTVIATFALSLAAGADRVQAATIANAAGGVVVGKRGTAV",
    "response#2": "HGRGEMLAMGGAGNVVSNIVSLGGAAIPVSVIGADQAGDNLMRMLGELGADTDGLTQRQDRMTSSKSRFSALNQQVLRFDEEEIKPLSRGERATLVEHFQAALARADIVILSDYGKGILLDGVAADLIAICRDAGKPVLVDPKGRDYARYAGATAVTPNRKELGEAVGRAVFGDDEITAAARDLIAVHDFEFIVATRSEKGMSVVTAEDARHISTQAREVFDVSGAGDTVIATFALSLAAGADRVQAATIANAAGGVVVGKRGTAG",
    "response#3": "HGRGEMLAMGGAGNVVSNIVSLGGAAIPVSVIGADQAGDNLMRMLGELGADTDGLTQRQDRMTSSKSRFSALNQQVLRFDEEEIKPLSRGERATLVEHFQAALARADIVILSDYGKGILLDGVAADLIAICRDAGKPVLVDPKGRDYARYAGATAVTPNRKELGEAVGRAVFGDDEITAAARDLIAVHDFEFIVATRSEKGMSVVTAEDARHISTQAREVFDVSGAGDTVIATFALSLAAGADRVQAATIANAAGGVVVGKRGTAA"
  },
  {
    "reference": "METNYSIPMSGSEVMVNDSTISRVLWILTMVVLSITFVLGVLGNGLVIWVAGFRMAHTVTTTCYLNLALADFFFTATLPFFIISTAMEGKWPFGWFLCKLLYIIADVNGIGSIFLITLIALDRCICVVHPVWAQNHRTVSLARKVVIGPWIFGLILSLHIFIFISTYRVSGGDVYCVYYFPSWGNTDEEMLNTVFIFTTALGIIKFIIIFIIPMSIVSICYGLIAVKIQRRALVNSSRASRVLKAVVASFFICWFPFQLVALLSLVWFKERLLNDEYKIIDMLIMPTNSLAFFNSCLNPILYVFFGQDFRKRLIHSLPSSLERALSEDSGQTSNTGTNSALPSANIEMMST",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must have at least one G-protein coupled receptors family 1 profile domain.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The designed protein should have scavenger receptor binding, RAGE receptor binding within plasma membrane to regulate positive regulation of monocyte chemotaxis, cellular response to amyloid-beta, G protein-coupled receptor signaling pathway. The designed protein sequence is ",
    "response#1": "GNGLVIWVAGFRMKHTVTTISYLNLAIADFCFTSTLPFYIASMVMGGHWPFGWFMCKFIYTVIDINLFGSVFLIALIALDRCICVLHPVWAQNHRTVSLAKKVIIVPWICAFLLTLPVIIRLTTVPNSRLGPGKTACTFDFSPWTKDPVEKRKVAVTMLTVRGIIRFIIGFSTPMSIVAICYGLITTKIHRQGLIKSSRPLRVLSFVVAAFFLCWCPFQVVALISTIQVRERLKNMTPGIVTALKITSPLAFFNSCLNPMLYAFLGRNFRKQLANLLALRAFVGRVRQPHPAPIHILLLSLTLADLLLLLLLPFKMVEAASDFLWHLPELLCAVTGFGFYSSIYCSVWLLAAISIERYLGVAFPVRYKLSRRPVYGVIAALVSWILSFGHCTIVIITQYLNSTQRLTNGSSIMCYENFTAEQLEVVIPVRLELFLVLFLIPMAVTIFCYWRFVWVMLTQPHVGAQRRRRAVGLAVVTLLNFLVCFGPYNVSHLVGFHTKVSPKWRAEAVVLSSLNASLDPLLYAFLGRNFRKQLSRVLS",
    "response#2": "GNGLVIWVAGFRMKHTVTTISYLNLAIADFCFTSTLPFYIASMVMGGHWPFGWFMCKFIYTVIDINLFGSVFLIALIALDRCICVLHPVWAQNHRTVSLAKKVIIVPWICAFLLTLPVIIRLTTVPNSRLGPGKTACTFDFSPWTKDPVEKRKVAVTMLTVRGIIRFIIGFSTPMSIVAICYGLITTKIHRQGLIKSSRPLRVLSFVVAAFFLCWCPFQVVALISTIQVRERLKNMTPGIVTALKITSPLAFFNSCLNPMLYAFIGVKFRRHLMQLLCRQRTS",
    "response#3": "GNGLVPWVTVFRMARTVSTVCFFHLALADFMLSLSLPILVYYIVSRQWLLGEWACKLYTGFVFLTFSTSNCLLVLISVDRCISVLYPVWALNHRTEQRASWLAFGVWLLAAALCSAHLKFRTTRKWNGCMQCYLQFNLENETAQMWTQEVFGRQMAVIMAHFLLGFLGPLAIIGTCAHLIRAKLLREGWVHANRPKRLLLVLVSALSAGSHLI"
  },
  {
    "reference": "MNMKKILRGPLIYILLAVAAVWIGTSLITASGFREVSTQQGLEFISNGDVKSAKIVDGEQRVDLTLTKEDKEFGTQVQFYYVTPRGLDVITAIDDASPAIEYTDEVPQGNWWLSMLGILLPLVLIGLFFWIMLSSMQGGGNRVMQFGKSKAKLVSKESPQVTFADVAGADEAIEELHEIKEFLKEPAKFQAVGARIPKGVLLYGPPGTGKTLLARAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFEQAKQNSPAIIFVDEIDAVGRHRGAGMGGGHDEREQTLNQLLVEMDGFDPKTNVIMIAATNRPDILDPALLRPGRFDRQIGVDAPDLKGRQKILQVHGKGKPLAAGVDLEVVARKTPGFTGADLANVLNEAALLTARSNAQLIDNRALDEAIDRVMAGPQRRTRVMRDREKLITAYHEGGHALAAASMNYTDPVTKITILPRGRALGYTMVMPLEDKYSVTRNELLDQLTYAMGGRVAEEIIFHDPSTGASNDIEKATSIARRMVTEYGMSANVGAVKLGQAQGEVFLGRDMGHQRDYSERIAERVDAEVRALIEKAHDEAWEVLNNNRDILDKLAAALLEQETLDHNQIAEIFTDVKKLPERPVWLSSDNRPVSDLPPIDFPHEKMPIDEGAVDGGVDSEPLPETEQSTPTWGTSRPATA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that can perform ATP binding, ATP hydrolysis activity, metalloendopeptidase activity, zinc ion binding, ATP-dependent peptidase activity in plasma membrane to support cell division, proteolysis.2. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.3. The protein should be able to bind Zn(2+), ATP ligand in a variety of conditions4. For general function, the protein need meet that Plays a role in the quality control of integral membrane proteins.5. The protein requires Zn(2+) to activate a critical catalytic site.6. The protein should have a Helical domain that is long enough to span the entire membrane.7. The designed protein should contain one or more AAA+ ATPase domains that are essential for its function.8. The active site active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "MMMIAAMITLLLVSYFMRSVTSTTQEITYTEFVNMVEDGKVARVEIEDSRIEIAPKDKSAKDTDAPIRYYTGKAEDDNTLTERLLTQKVIVEAPVAEAPVQEAPVEEAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKASPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVKAAPVK",
    "response#2": "MMMIAAMITLLLVSYFMRSVTSTTQEITYTEFVNMVEDGKVARVEIEDSRIEIAPKDKSAKDTDAPIRYYTGKAEDDNTLTERLLTQKVIVEAPTDAPTNGGILMQIFISWFPMLLLIGVWIFFMRQMQGGMGGKGAMSFGKSKARMLSEDQVKVTFADVAGCDEAKEEVAELSDFLRDPGKFQRLGGKIPKGVLLVGPPGTGKTLLAKAVAGEAGAPFFSISGSDFVEMFVGVGASRVRDLFKQAREKAPCIIFIDEIDAIGRARGRGNMQGGNDERENTLNQLLVEMDGFSTDKGVILMAATNRPDVLDSALLRPGRFDRQIGIDRPDIKGREQILKVHMRKVPIGDTDVIARGTPGFSGAELANLINEAALLAARRNKRVVGMEELEQAKDKIMMGAERRSMVMREDERRNTAYHESGHAVVAKLLPNADPVHKVTIMPRGWALGLTWQLPEFDRFSSFKEKMLEEIAILFGGRIAEEVFMKQMSTGAANDFERATKLARDMVTKYGMSDKLGTMVYSDDTNDGFMSMSSKSISEATQQKVDAEIKSILDQQYAVARKLIEKNKKKIELMAKALLEYETIDSDQINDIMAGRPPRPPKGWDDSSEDESSGKKSSKKSGDDSKAESL",
    "response#3": "MMMIAAMITLLLVSYFMRSVTSTTQEITYTEFVNMVEDGKVARVEIEDSRIEIAPKDKSAKDTDAPIRYYTGKAEDDNTLTERLLTQKVIVEAPVAEAAPAAEEPAKDE"
  },
  {
    "reference": "MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLAQALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFSGRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAELCAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: It seems to be involved in an RecBC-independent recombinational process of DNA repair; It may act with RecF and RecO.; May play a role in DNA repair.2. The protein design must be able to perform metal ion binding, DNA binding in order to contribute to DNA synthesis involved in DNA repair, response to radiation, response to UV.3. The designed protein have Toprim domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "IICVVEELGDLWAFERGRIYSGVYHVLGGALSALSGIGPEDLNLGGIVDRIRTHGVKEVIVATSNDMDGQVTCHYIAQMVKGTDAKVTRLR",
    "response#2": "TLLCVVETAADVMAIEQTAGYRGRYFVLGGHLSPIDGISADDLNIEQLVWRVKQEPVEELILATGTTVEGQTTAHFISEAVSRHVNKVTRLAQGVR",
    "response#3": "LCVVETAADVMAIEQTAGYRGRYFVLGGHLSPIDGISADDLNIEQLVWRVKQEPVEELILATGTTVEGQTTAHFISEAVSRHVNKVTRLR"
  },
  {
    "reference": "MAETMKDITMKNDESQEEEIPDQFLCCVCLELLYKPIVLSCGHLSCFWCVHKSMNGFRESHCPICRDPYVHFPSVCQKLYFLLKKMYPLAHKKREEQVLKEEQERECFSPQIDLVLDLSVCSGDSLNVSDKQKVEECSNAANLLSSSSSRGDIPCIPKNQEPTDAKALNVHENELLKDNKVSKQISKDDLLCSACKELLVRPVVLNCGHVYCEGCVVDMAEESEKIKCQECNVCDPRGFPKVCLILEQLLEENFPEEYNSRSSKVQKTLAHNSKGNIQSYLKEGPSLSNDNNNDDPWLANPGSNVHFGAGCDSCGVYPIIGDRYRCKDCKEEIGYDLCKDCYETPSKVPGRFNQQHTPDHRLELARSPQVLINFNSIGILLGPVISNEGMDTDEGEEGPPGSSNESSSTE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Functions in the N-end rule pathway of protein degradation, where it specifically recognizes and ubiquitinates proteins with a N-terminal bulky aromatic amino acid (Phe)2. The designed protein should have zinc ion binding within cytoplasm to regulate defense response to fungus, proteasome-mediated ubiquitin-dependent protein catabolic process. The designed protein sequence is ",
    "response#1": "FATASKAHGEGIKRALQGKPASFTVVGYDHDGEPRLSGGDSVSVVLMSPDGNLSSAEVSDHQDGTYTVSYLPKGEGEHLLSVLICNQHIEGSPFKVMVKSD",
    "response#2": "EHYQLYLRYQNARHAGGGMDQDSIDQYTQFLLQSRVNSRLVEFRETGADGQPGTLKMVSILDVLEDGLSAVYTFYEPEPGASYGTYNVLWQVQQARTLGLPYVYLGYWIEQSPKMNYKARFMPHER",
    "response#3": "FATASKAHGEGIKRALQGKPASFTVVGYDHDGEPRLSGGDSVSVVLMSPDGNLSSAEVSDHQDGTYTVSYLPKGEGEHLLSVLICNQHIEGSPFQ"
  },
  {
    "reference": "MEQSKERRSVYQELRVFSGNAHPDLAQAICDYLEMPLGQSEAFKFSNENIFVRFCENIRARDVFLVQPIVSPVNDRLMELFIMIDAAKRASAGRITAVVPYYAYGRSDKKDQPRVPITARLVANFMETAGADRLLTVDLHAGQIQGFFNIPVDELTALYILSRYFKDKGIPSLTVLATDVGDAKRARNVAGRLNAPLAIVEKRRIGNEQRAEAVNIIGEVKGRNVLIVDDEVDTGSTLVATMDLLQQQGTVDIYACCTHPVLSGPAIERIEQSPIRELVVTDTIPLPIEKRLPKITVLSLAPLLGEAIQRIHTGHSVGELFQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that is capable of catalyzing reactions within the 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1, 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis pathway with high efficiency and specificity.2. The protein design should aim to mimic or enhance the function of natural proteins involved in 5-phosphoribose 1-diphosphate biosynthetic process, nucleotide biosynthetic process, ribonucleoside monophosphate biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.3. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+), ATP binding. The designed protein sequence is ",
    "response#1": "MKIFAGRNSKDLAHRICAEMDVELGRARTTLFPDGEVFVKLDDDVRGRDCYVVISTCDPVNDNLMELLVFCDCLNRASANTVTMVMPYYGYGRQDRKDEGRVPITAKLVANLITEGRIRDLHAGQIQGFFNIPVGNLHMAVPVLHHYIEKLELDPDELAIVSPDEGRIKLALKLQRRLGGTLAVIDKRRSSATETDQVSIIGGPVDGKIAIMFDDMISTGGSVVGAARVLHASGARDIRVCVTHGLFCNNALERMRQSPLVQIVATDAIPLIGDYLIENYGEKDVVVVAPDHSGVVRARRIADRLNAPIAILNRKPRPHEDEIMSVIGDVKGKVAIVVDDIIDTGVRATTSADILLEKGAVEVIACATHSVMAGNATERLQNSNIKEVITSDPILARYFEESLQGEDRETLTVVSPDMGRVRVADTWADEFGAPLAIIHKRRDPKVANQVTVHDIVGEVEGRTCLLVDDMIDTGGTIVKAAQALKANGARKVIVAATHPIFSNPAAERLQDAAIDEVVVTDTIPLPPQKKWDELFVISKVG",
    "response#2": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAERGVRNVDLHAAQIQGFFDIPIDHAVPVLHHYIEKLELDPDELAIVSPDEGRIKLALKLQRRLGGTLAVIDKRRSSATETDQVSIIGGPVDGKIAIMFDDMISTGGSVVGAARVLHASGARDIRVCVTHGLFCNNALERMRQSPLVQIVATDAVPVLHHYIEKLELDPDELAIVSPDEGRIKLALKLQRRLGGTLAVIDKRRSSATETDQVSIIGGPVDGKIAIMFDDMISTGGSVVGAARVLHASGARDIRVCVTHGLFCNNALERMRQSPLVQIVATDTLFPGQIATPVLIEDIKRQRFENYVVVSPDVGGVVRARAIAKRLDDADLAIIDKRRPKANVSQVMHIIGDVRGKTCVLVDDIVDTSGTLCKAANALKEHGAEHVVAYITHPVLSGPAIENIEASSLDELVVCDTVYAPGSKLDRAAFALKVLTGSANRELAREVCEYLGIEPVRMRCGRFPDGEIDVKIEEDMRGCDAFVVQPTCPPVNENLMELLVILDCLRRASAGRITAVLPYFGYARQDRKAEGRVPITAKLVANLLTVAERGVRDV",
    "response#3": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAETGIERMATIDLHAGQIQGFFNIPVDHLYATPVLIEDIKRQRFENYVVVSPDVGGVVRARAIAKRLDDADLAIIDKRRPKANVSQVMHIIGDVRGKTCVLVDDIVDTSGTLCKAANALKEHGAEHVVAYITHPVLSGPAIENIEASSLDELVVCDTVEVPETKRLKNVKVLPLSELIAEAIRRTHNGESVSYLFE"
  },
  {
    "reference": "MRKTLSILAISLPLLVSGYAQASESGVLKAKLAEISTLKANFTQTVTDVNKKEIQKGSGVFALAYPNQFYWHLTEPDESLIVADGRDVWIYNPFAEEVSVMDLNQAINASPIALLVHRDEETWSKYTVTNEEGCFSIAPKSVDAGVESVDVCFDGNTLTQMVLQDQQGNVSQFMLSDQTAISDDELSMFKFTVPDDVDIDDQRLNTVN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Participates in the translocation of lipoproteins from the inner membrane to the outer membrane.2. The protein should have a specific function in lipoprotein transport and should be localized to periplasmic space in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "VQRWL",
    "response#2": "MASK",
    "response#3": "MF"
  },
  {
    "reference": "MIGKEVTVQDFTLELSELQPEVLPVDLLCEEELPNEQETEEESDIDRTVFKIIAPCGCSSCEVNLRIFVNATDTGIRTLQDLLISDLQLLCPECRGNCKHGGF",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that localizes to host cell cytoplasm, host cell nucleus and can perform DNA-binding transcription factor activity, DNA binding with high specificity and efficiency.2. The protein must exhibit the following characteristics: Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta).; Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation; Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. The designed protein sequence is ",
    "response#1": "GPESXR",
    "response#2": "SCPFGCKF",
    "response#3": "MIWLL"
  },
  {
    "reference": "MGGKWSKSSIVGWPQVRDRIRRTPPATEGVGAVSQDLDKHGAVTSSNMNNADSIWLRAEEEEGEGVGFPVKPQVPLRPMTFKGAFDLSFFLREKGGLDGLIYSKRRQEILDLWVYHTQGFFPDWQNYTPGPGIRFPLCFGWCFKLVPVDPREVEEENKGDTNCLLHPMSQHGIEDDEGEVLMWKFDSALARRHIAREQHPEYYKDC",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis.2. A protein that contributes to suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host autophagy, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I by functioning in virion component, extracellular region, host cell plasma membrane, membrane, host cell Golgi membrane with GTP binding, SH3 domain binding.3. The protein design must feature the Diacidic; necessary for CD4 internalization, PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif. The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPAVRERIRRTEPAAEGVGAASQDLDKYGALTTSNTASNNAACAWLEAQEDEEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGTRFPLTFGWCFKLVPVDPREVEEATEGENNCLLHPICQHGMEDEEREVLMWKFDSSLARRHIARELHPEYYKDC",
    "response#2": "MGGKWSKSSLVGWPRIRERIRKTAPAADGVGAASQDLDKHGALTSSNTPTTNADCAWLEAQEEEEVGFPVKPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQKRQDILDLWIYNTQGYFPDWQNYTPGPGTRYPLTFGWCFKLVPVEQEKVEEVNEGENNSLLHPMSLHGMEDPEKEVLMWKFDSRLAFHHMAREKHPEYYKDC",
    "response#3": "MGGKWSKSSIVGWPEIRERMRRAPTAAEGVGAVSQDLEKHGAITSSNINHPSCVWLDAQEDEEVGFPVRPQVPLRPMTFKAAFDLSFFLKEKGGLDGLIYSKKRQEILDLWVYNTQGYFPDWHNYTPGPGVRYPLTFGWCFKLVPVDPAEVEEANEGENNCLLHPICQHGMEDEDREVLMWKFDSSLARRHVARELHPEYYKDC"
  },
  {
    "reference": "MSTSSVRFAFRRFWQSETGPKTVHFWAPTLKWGLVFAGFSDMKRPVEKISGAQNLSLLSTALIWTRWSFVIKPRNILLASVNSFLCLTAGYQLGRIANYRIRNGDSISQLCSYILSGADESKKEITTGR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Mediates the uptake of pyruvate into mitochondria..2. A protein that contributes to mitochondrial pyruvate transmembrane transport by functioning in mitochondrion, inner mitochondrial membrane protein complex, mitochondrial inner membrane with pyruvate transmembrane transporter activity.3. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "GAPE",
    "response#2": "MGLKV",
    "response#3": "MGHRKL"
  },
  {
    "reference": "MSKFLIKLITLYQKWISPLKGQTCRFYPTCSSYSISAYNTYGFFRGTYLTIRRLLKCHPFHPGGFDPLK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be expressed specifically in the plasma membrane2. The protein must exhibit the following characteristics: Could be involved in insertion of integral membrane proteins into the membrane.. The designed protein sequence is ",
    "response#1": "MSII",
    "response#2": "MPRSFLVR",
    "response#3": "GNTTCF"
  },
  {
    "reference": "MKIKTRFAPSPTGYLHVGGARTALYSWLFSRHAGGEFVLRIEDTDLERSTQDAIDAIMDGMNWLNLDWNEGPYFQTKRFDRYNAVIDDMLVQDTAYKCYCSKERLEALREKQMENGEKPRYDGHCRDSQCSHTDDEPHVVRFRNPQEGSVIFDDKIRGPIEFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEISHVIRGEDHINNTPRQINILKALGAPVPEYAHVSMILGDDGKKLSKRHGAVGVMQYRDDGYLPQALLNYLVRLGWSHGDQEIFSIDEMKEFFTLEAINKSASAFNTEKLQWLNHHYINHMPAEEVAVHLAWHVEQLGIETRNGPELKDIVKLLGERCKTLKEMAESCRYFYEDFSEFDADAAKKHLRPVARQPLEAVRAKLAAITAWTPENVHDAIQGTADELGVGMGKVGMPLRVAVTGAGQSPGMDVTVHAIGQNRSLQRIDLALAYIAGREA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. We are looking for a protein with broad substrate specificity for ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-   glutamyl-tRNA(Glu).2. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.3. For general function, the protein need meet that Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).4. The protein should be able to bind Zn(2+), ATP ligand in a variety of conditions5. The protein design should include a 'KMSKS' region, 'HIGH' region motif to ensure proper function.6. The protein can be expressed in cytoplasm and perform glutamate-tRNA ligase activity without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "MVKTRFAPSPTGYLHIGGARTALFSWAFARKQGGKFVLRIEDTDLERSTQQSVQAILDGMAWLGLDYDEGPYYQMQRLNRYQEVAEQLLTQGLAYQCYASREELDALREQQRLAGLKPRYDGRWRDSRQTPPAGVKPVVRLKTPQHGYVVFDDLVKGKISVANHELDDLVLLRSDGTPTYNFGVVLDDLDMGITHVIRGDDHVNNTPRQINILKALGAAIPQYAHVPMILGADGERLSKRHGAVSVMHYRDQGYLPEALINYLARLGWSHGDEEIFSREQLVEWFDLAAISRSPAKFNPEKLTWLNQHYLKQPEDRAFLQHALDTLPAEPWGEETWKDWTNALKEQSGRKGRSLFMPLRLALTGEDAGPELHVLLGLMGRERTVARLRDAL",
    "response#2": "MVKTRFAPSPTGYLHIGGARTALFSWAFARKQGGKFVLRIEDTDLERSTQQSVQAILDGMAWLGLDYDEGPYYQMQRLNRYQEVAEQLLTQGLAYQCYASREELDALREQQRLAGLKPRYDGRWRDSRQTPPAGVKPVVRLKTPQHGYVVFDDLVKGKISVANHELDDLVLLRSDGTPTYNFGVVLDDLDMGITHVIRGDDHVNNTPRQINILKALGAAIPQYAHVPMILGADGERLSKRHGAVSVMHYRDQGYLPEALINYLARLGWSHGDEEIFSREQLVEWFDLAAISRSPAKFNPEKLTWLNQHYLKQPEDRAFLQHALDTLPAEPWGEETWKDWTNALKEQSGRKGRSLFMPLRLALTGEDAGPELHVLLGLMGRERTVARLRDALA",
    "response#3": "MVKTRFAPSPTGYLHIGGARTALFSWAFARKQGGKFVLRIEDTDLERSTQQSVQAILDGMAWLGLDYDEGPYYQMQRLNRYQEVAEQLLTQGLAYQCYASREELDALREQQRLAGLKPRYDGRWRDSRQTPPAGVKPVVRLKTPQHGYVVFDDLVKGKISVANHELDDLVLLRSDGTPTYNFGVVLDDLDMGITHVIRGDDHVNNTPRQINILKALGAAIPQYAHVPMILGADGERLSKRHGAVSVMHYRDQGYLPEALINYLARLGWSHGDEEIFSREQLVEWFDLAAISRSPAKFNPEKLTYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEAEKEVMAGETVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPNTIYLLGRDKSIEHIKYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEAEKEVMAGETVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPNTIYLLGRDKSIEHIKAALA"
  },
  {
    "reference": "MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAHKLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTIYQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYSTVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be designed to catalyze the following reaction acetyl-CoA + L-serine = CoA + O-acetyl-L-serine.2. A protein that is capable of catalyzing reactions within the L-cysteine biosynthesis, L-cysteine from L-serine: step 1/2, Amino-acid biosynthesis pathway with high efficiency and specificity.3. The designed protein should be involved in cysteine biosynthetic process from serine by localizing to cytoplasm and exhibiting serine O-acetyltransferase activity. The designed protein sequence is ",
    "response#1": "PRYRLAVERDAEQLLELTLRAYEPIRKLGIRFAAAHADLDLVLKNIRENACYVMEEDGRIIATITLRMPWGKQPGPYGVPHIWWFAVDPDTGKKGIGTKLLQWLEETILRDTLKVPFVSLGTADKHPWLIEMYERKGYVRSGEQDLGKGHITVYMKKQLPW",
    "response#2": "EELIGNTPLVKIRSLTKATGVNIYAKLELCNPAGSAKDRVALNIIKTAEELGELVRGEPGWVFEGTSGSTGISIAVVCNALGYRAHISLPDDTSLEKLALLESLGATVNKVKPASIVDPNQYVNAAKKACNELKKSGNGIRAVFADQFENEANWKVHYQTTGPEIAHQTKGNIDAFIAGCGTGGTITGVAKFLKERAKIPCHVVLADPQGSGFYNRVNYGVMYDYVEKEGTRRRHQVDTIVEGIGLNRITHNFHMGEKFIDESIRVNDNQAIRMAKYLSVNDGLFVGSSTAINAVAAIQVAKTLPHGSNIVIIACHGDC",
    "response#3": "EELIGNTPLVKIRSLTKATGVNIYAKLELCNPAGSAKDRVALNIIKTAEELGELVRGEPGWVFEGTSGSTGISIAVVCNALGYRAHISLPDDTSLEKLALLESLGATVNKVKPASIVDPNQYVNAAKKACNELKKSGNGIRAVFADQFENEANWKVHYQTTGPEIAHQTKGNIDAFIAGCGTGGTITGVAKFLKERAKIPCHVVLADPQGSGFYNRVNYGVMYDYVEKEGTRRRHQVDTIVEGIGLNRITHNFHMGEKFIDESIRVNDNQAIRMAKYLSVNDGLFVGSSTAINAVAAIQVAKTLPHGSNIVIIACG"
  },
  {
    "reference": "MTNERQNTPFENSQADTIRILIASDNHVGYAEKDPVRGNDSFVSFDEILKIAREKEVDMVLLGGDIFHDNKPSRKAFYQALRSLRLNCLGDKPCELELLSDTSVTTGDTTICNVNYEDPNINVAIPVFSIHGNHDDPSGDGRYSALDILHVTGLVNYFGRVPENDNISISPILLQKGSTKLALYGLSNVRDQRLYHSFCENKVKFLRPDLYRDEWFNLLVVHQNHAAHTETSYLPESFIQDFYDFVLWGHEHECIIDGTYNPTQKFTVVQPGSSVATSLSPGETVPKHCGILHVTGRDFFMEKIRLRTVRPFVMRDIVLSEVQSIPPMVDNKREVLTYLISQVEEAIEEANQQWLEAQGGQDNVQDKKPPLPLIRLRVDYTGGYQTENPQRFSNRFVGRVANATDVVLFHLKRKYTRTKKNNEVVDNVGEDIKINALRVESLVNEYLKKNQLECLPGNVLGEAVINFVEKDDKDAIKECVEDHLSKQINLLVKKRVTEENLEEEIKTLVNNIPQLSTTKRKEYDSMPTPASTSFRVTSPDSDFDDDRPLLSANRLDENEEKDDFQSLTQAEPPKQSSKTTKTRALPGSMYQKPANTKKPSLKSSGPKKTSGRTQGKRTDKPAEKSTQLMLQDSFSSSQKPASQYLDDDDEILDD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Involved in DNA double-strand break repair (DSBR); Possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity; Also involved in meiotic DSB processing..2. The designed protein should have single-stranded telomeric DNA binding, manganese ion binding, double-stranded telomeric DNA binding, endodeoxyribonuclease activity, molecular adaptor activity within chromosome, telomeric repeat region to regulate meiotic DNA double-strand break formation involved in reciprocal meiotic recombination.3. Include a signal peptide as an essential component of the protein.4. The presence of Mn(2+) is crucial for the protein to function optimally under specific conditions.5. The designed protein have Mre11 DNA-binding domains with specific sequence motifs that are crucial for its activity.6. The designed protein must have a Proton donor that is structurally stable and resistant to denaturation.7. The designed protein's functionality will benefit from Polar residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "MNMQKIPLQTVRQFFMDDVVLANHPDIFNPDNPKVSQAIQNFCLEKVEEMLDKAERERLGNPQRPEKPLIRLRVDYSGGFEPFSVLRFSQKFVDRIANPKDIIHFFRHREQKEKTDEDINFGKFIRKPASEGMTLRVEDLVKQYFQTAEKNVQLSLLTERGMGEAVQEYSIEAIELKTVRPFVIKDIELSKTDLIPGAASRSDVIAYLTEEAEKAIEEANRVFKEKNGHLFTGESESKLPLPLVRLRVEYSGGFEIENVTRFSNRFVGKVANVNDVVQFYKRKVPPRQKTSLTKKTKFDADLLEEKLSERKANDLKLKDIVSDMLKQTQLTLVPEDGINEAVQKFFMEKIRLRTVRPFVMKDIVLSEVQSIPPMVDNKREVLTYLISQVEEAIEEANQQWLEAQGDDPAVLNEKHPLPLIRLRVDYTGGYQTENPQRFSNRFVGRVANATDVVLFHLKRKYTRTKKNNEVVDNVGEDGKINALRVESLVNEYLKKNQLECLPGNVLGQAVINLT",
    "response#2": "MNMQKIPLQTVRQFFMDDVVLANHPDIFNPDNPKVSQAIQNFCLEKVEEMLDKAERERLGNPQRPEKPLIRLRVDYSGGFEPFSVLRFSQKFVDRIANPKDIIHFFRHREQKEKTDEDINFGKFIRKPASEGMTLRVEDLVKQYFQTAEKNVQLSLLTERGMGEAVQEGADGSGNGDEYILVVEKETVFQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKLHLPVHCLVDCDPYGFEILATYRFGSMQMAYDIESLRAPDMKWLGAFPSDSEVYSVPKQCLLPLTEEDKKRTEAMLLRCYLKREMPQWRLELETMLKRGVKFEIEALSVHSLSFKCEPIRLKSVRPFAMREIILSEEKGAQKLARKDNNRTEVTRLLMEIVEELIEEAKTEWLEMQDETAEDEELEVPLPLVRLRVETSTPEGGSYDCENPQRFSNRFVGKVANVNDVVQFYRKKKNTNSTRKPGETDLDETAVSHLSTLDTVKVEQLVRDFLSAQSLTILPQNSFGDAVAQGACTKILLATDNHIGYLERDPIRGQDSINTFREILQLAVKNEVDFILLAGDLFHENKPSRDCLYQTLALLREYTLGDKPIQVELLSDPDEGKAAGFSFPAINYEDPNFNISIPVFSIHGNHDDPQGPGVNGALCALDVLSVSGLLNYMGKFDLPTSDADAATTGIAVRPVLLRKGSTKLGMYGVGNVKDQRMHFELRSNRVRMYMPKDKDEWFNILLVHQNRVKHGPQEYVPEGMFDDSVDLVVWGHEHDCRIIPEPVAGKNYYITQPGSSVATS",
    "response#3": "MNMQKIPLKTVRQFFIEDIILADHPNIFNPNSLKVTQAIQNFCLEKVEEMLENAERERLGNPRQPEKPLIRLRVDYTGGFEPFNVLRFSQKFVDRIANPKDVIHFFRHREQKEKTGEEINFGELITKPPSEGPTIRVEDLVKQYFQTAEKKLQLSLLTERGMGEAVQEESPGAAAAASAAAAAAPQQPAAGGSKPLNGRKEQTLKIYNNLNSIEDDRCPICGRPLDSEHKAKIREEIKVQLLELNKQNSYSIEAIELKTVRPFVIKDIELSKTDLIPGAASRSDVIAYLTEEAEKAIEEANRVFKEKNGHLFTGESESKLPLPLVRLRVEYSGGFEIENVTRFSNRFVGKVANVNDVVQFYKRKVPPRQKTSLTKKTKFDADLLEEKLSERKANDLKLKDIVSDMLKQTQLTLVPEDGINEAVQKPERKKIFSGKTFVFLTAKQHKKLGPAVILGGGEAKLMAEERKETSLLVSPEVCVVDVGVTNSQILGSESMRNWTDSILAVLESNNLRAIPEAEIGLAVIFMSTEIYCNEYILVVEKETVFQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKLHLPVHCLVDCDPYGFEILATYRFGSMQMAYDIESLRAPDMKWLGAFPSDSEVYSVPKQCLLPLTEEDKKRTEAMLLRCYLKREMPQWRLELETMLKRGVKFEIEALSVHSLSFLSEVYIPSKYSIEAIELKTVRPFVIKDIELSKTDLIPGAASRSDVIAYLTEEAEKAIEEANRVFKEKNGHLFTGESESKLPLPLVRLRVEYSGGFEIENVTRFSNRFVGKVANVNDVVQFYKRKVPPRQKTSLTKKTKFDADLLEEKLSERKANDLKLKDIVSDMLKQTQLTLVPEDGINEAVQK"
  },
  {
    "reference": "MERQTGARNVIVGVSGSIAAYKAPSVIRLLRREGHEVRTVATEAALRFIGAPALAAVTGRPVSTGVFDDPAAVEHVEMGEWAELVVVAPASADLLARLAAGRADDLLTATILMTTAPVVLAPAMHTRMWTSPATEENVATLRRRGVSVIPPDAGPLTGGDSGAGRLPEPGRIVSAALAILGAAGDAVPIADAAGSAAGSRPPQDLAGRALVVSAGGTREPLDPVRFLGNRSSGRQGCAIAAEAARRGARTVVVAANVDAHVLAGLEPEGVRVIPVGTALELGETVRGEAAQADAVIMTAAVADYRPATVSASKIKKRGMSRSPDCGATPARRVIELVENPDVLTGLVADPPRRGLLVVGFAAETGDAAGDVLAHGAAKARRKGADLLAVNAVGARTGFGDVPNSVVVLDSDGREVVRAAGSKAEVARTLVDLVARVLDARVQDS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should contain one or more Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains that are essential for its function.2. The presence of Mg(2+) is necessary for the protein to function in the desired environment.3. The protein design should take into account the physiological conditions of the Cofactor biosynthesis, coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway.4. A protein with phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity, FMN binding functions is required to enable pantothenate catabolic process, coenzyme A biosynthetic process.5. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A6. The protein should be able to bind CTP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "KNIVLGVTGGIAAYKSLEVVSRLKKLGAEVDVIMTKSATEFVQPLSFRSLSHNPVIVDMFAEPKEWDVKHIALAQKADIFVIAPATANIIGKIANGIADDMLSTTVMATTAPVVLAPAMNENMYNNHIVQDNLEELSKLGYQIIEAASGYLACGVEGKGRLPEPKEIVTYLETHILDKLPTTKQVVITAGPTREIIDPVRYLTNRSSGKMGYALAEAATVMGATVTLISGPTNLLSSKKINRINVITAQEMFTAVMHEIAQCNIFISAAAVSDYRPTLIETTKIKRNKTNLTLNLIRNPDIISRVSCLPNKPFIVGFALETANHLKNAQIKLKQKNMDMIVLNSEKACEGDKNAVTVELLFKDKTQKKFPLMSKTEVAEAILDEVITLLKE",
    "response#2": "KKILLGVSGGIAAYKTASLVRLFIKAGAHVQVIMTPASKDFVTPLTLSTLSKNPVYSTFYNEEDADAQWNNHVELGLWADLMVIAPATANTLSKMTNGNCDNLLIATYLSAKCPVYFAPAMDLDMYKHPSTIASFTALNQYGNIMIPAESGELASGLSGEGRMAEPENIVAQDLIDHALTSQLTGRKVLITAGGTREPLDPVRFIANRSSGKMGYALATEAVRRGGQVVLVSGPVELAPPPGLAEFIRVERAEEMAEAVLSRAPEMDVIIKAAAVADFRPVQVAEQKVKKGDGDWVLHLQKNPDILAILGARKRPGQVLVGFAAETHDLLANGAEKARRKGADFIVANDVSRPGSGFGSDTNEVTFLFDDSGTHELARGSKHQLAHAVWDAVVQRW",
    "response#3": "KRILLGITGGIAAYKSAELVRLLKKAGCEVRVVMTRAAEAFVTPLTFQALSGEPVRTSLLDPEAEAGMGHIELAKWADQVVVAPASADFLARLAQGLADDLLTTVCCATEAPIILAPAMNQAMWRNPRTQRNVALLRDDPQVCLWGPDQGEQACGDTGPGRMLEPAELAARLLDALGVRRGGLDGRHVVVSAGGTREALDPVRFLGNRSSGLQGYALARTAAARGARVTLVAANVALPDPAGVRVVVVESALQLREAVLSAADRADAVVMAAAVADFRPAGYRPSKIKKRDGASAPSIELVQNPDVLAELGARPHAGQVIVGFAAETDDVLANGRAKLARKGCDLLVVNQVGADLAFGRPDNAAVGFLDAEGRETPLPQASKLALAHRIWDLVAERLEADR"
  },
  {
    "reference": "MLWQRLAVVEWAALAWELLGASVLLIAVRWLVRRLENLSRDPNRCGTLSSLPGASAAVSAQPGEVMTMDAMLARLKLLNPDDLRQEVMKAGLKCGPITSTTRFIFEKKLAQALLEQGGLLTSSLPKHSEVTGTAFIHGTSRTPAFVDRKQTQQACLSEDRDFGYSVGLNPPEEEAVTSSVHPIPFSASTRNDNHKAGVTTAKEPLLYYGVCPVYEDGLVRHERIHVYEDKKEALQAVKLIKGSRFKAFPTREDAEKFARGICDYLPSPSKNMSLLSPVKAMPLCSNDGPKADGLCLAESETVNKERANSYKNPRTQDLTAKLRKAVERGEEHTFSDLIWSNPRYLIGSGDNPTIVQEGCRYNVMHVAAKENQASVCQLTLETLENPEFMRLMYPDDNMDMLQKRILYIVDLYLNTPDKVGFDTPLHFACKFGNVDVVNVLSSHPLIVKNPRNKYGKTPEDVICERSKNKSVELRERIREYLMGHYYVPLLRAEDTSPVIGELWSSDQKAEASSADHCRSSPRDPVMTLRAFVGPLSPSKAEDFRKLWKTPPRKKAGLFHSIRKSDPERGIERVGRELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLEEYLIQKELNQKAQHEIGEEEGCLQDRTSDFGSGKKYSNSISVGAFLDGGDDISLEEVKNQQNTVPSQSQPSVDKFQNSKSGSPSLGQKADPGEAAVGFYPDNSRNGFCYPLNSRTADGRETEATNGEEALPPPVSILTQEFDKLNLQSLGDNLFETPNKN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.2. The protein must exhibit the following characteristics: It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex; Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly.3. A protein that can perform protein phosphatase 2A binding in endoplasmic reticulum to support negative regulation of phosphorylation, central nervous system development.4. Target a Polar residues compositional bias in the protein's composition for improved properties.5. The protein should contain Helical; Signal-anchor for type III membrane protein transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "VGKQFATGGFGRIHTCTEDGKSQQMVMKIEPSTNGPLLTEVVVFNRILKKDMIENYKKAKKIQWIGLPHLIANGYFTYNNEKMRYMIIPKYATSLEAVREANGGSLSAKDSLTVADCVLGALEYLHDSDYAHADVKAANILLEKPNDFSSAVLVDFGLAHRTTNNVDKPDKKRAHNGTCIFTSTDAHRGNNPSFRGDVEILAYNLIMWISGSLSKLPRKTLVLDLDETLIHSVSRGSRTTSGQPIEVHVPGEHPILYYIHKRPHLDYFLSNVSQWFRLILFTASVQPYADPIIDYLERDKKIFAKRYYRQHCALVDSSFVKDISICNIHLSRIMIIDNSPASYNAHKENAIPIEGWISDPSDVDLLNLLSFLHAE",
    "response#2": "VGKQFATGGFGRIHTCTEDGKSQQMVMKIEPSTNGPLLTEVVVFNRILKKDMIENYKKAKKIQWIGLPHLIANGYFTYNNEKMRYMIIPKYATSLEAVREANGGSLSAKDSLTVADCVLGALEYLHDSDYAHADVKAANILLEKPNDFSSAVLVDFGLAHRTTNNVDKPDKKRAHNGTCIFTSTDAHRGNNPSFRGDVEILAYNLIMWISGSLPRKTLVLDLDETLIHSVSRGSRTTSGQPIEVHVPGEHPILYYIHKRPHLDYFLSNVSQWFRLILFTASVQPYADPIIDYLERDKKIFAKRYYRQHCALVDSSFVKDISICNIHLSRIMIIDNSPASYNAHKENAIPIEGWISDPSDFLRTPPVKTLVLDLDETLIHSVSRGSRTTSGQPIEVHVPGEHPILYYIHKRPHLDYFLSNVSQWFRLILFTASVQPYADPIIDYLERDKKIFAKRYYRQHCALVDSSFVKDISICNIHLSRIMIIDNSPASYNAHKENAIPIEGWISDPSDVDLLNLLSFLHALQYVHDVL",
    "response#3": "VGKQFATGGFGRIHTCTEDGKSQQMVMKIEPSTNGPLLTEVVVFNRILKKDMIENYKKAKKIQWIGLPHLIANGYFTYNNEKMRYMIIPKYATSLEAVREANGGSLSAKDSLTVADCVLGALEYLHDSDYAHADVKAANILLEKPNDFSSAVLVDFGLAHRTTNNVDKPDKKRAHNGTCIFTSTDAHRGNNPSFRGDVEILAYNLIMWISGSLF"
  },
  {
    "reference": "MELISNELLYKTYKQKPTGVEEPVYDRLGNPLFGERGEIHPQSTLKLPHKRGELEIPTNVKSLPKRGDCRTGNNKGPVSGIYLKPGPQFYQDYTGPVYHRAPLELFEEGSMCEVTKRVGRVTGSDGRLYHIYVCVDGCILVKSATRNNPKVLKWVHNRLNCPLWVTSCSDKQGEGAKKKTQQKPDRMERGKMKITPKESEKDSKTRPPDATIVVDGVKYQVKKKGKVKSKNTQDGLYHNKNKPPESRKKLEKALLAWAILTVVLFQVVMGENITQWNLQDNGTEGIQRAMFQRGVNRSLHGIWPERICSGVPSHLATDVELKAIHGMMDASEKTNYTCCRLQRHEWNKHGWCNWYNIEPWILLMNKTQANLSEGQPPRECAVTCRYDREADLNVVTQARDSPTQLTGCKKGKNFSFAGVLTPRPCNFEIAVSDVLFKEHDCTNVLQDTAHYLVDGMTNSLEGARQGTAKLTTWLGKQLGILGKKLENKSKTWFGAYAASPYCDVDRKVGYIWYTKNCTPACLPKNTMIIGPGKFDTNAEDGKILHEMGGHLSEMLLLSLVVMSDFAPETASVIYLILHFSIPQGHTEVLDCDKNQLNLTVGLTTADVIPGSVWNLGRYVCIRPDWWPYETATVMAFEEVGQVIKIALRALKDLTRIWNAATTTAFLVCLVKVVRGQVLQGILWLLLITGVQGLPGCKPDYSYAIAKNTEIGPLGAEGLTTTWHEYSAGMRLSDTMVDVWCKGNVLMFYERCVRETRYLAILHVRALPT",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Plays a role in the regulation of viral RNA replication..2. The designed protein have RdRp catalytic, Peptidase C53, Peptidase C74, Peptidase S31 domains with specific sequence motifs that are crucial for its activity.3. The protein should have a Helical domain that is long enough to span the entire membrane.4. The designed protein should be involved in protein complex oligomerization, fusion of virus membrane with host endosome membrane, viral RNA genome replication, viral entry into host cell, proteolysis by localizing to host cell surface, host cell membrane, host cell cytoplasm, cytoplasm, virion membrane and exhibiting ribonucleoside triphosphate phosphatase activity, ATP binding, ribonuclease T2 activity, serine-type exopeptidase activity.5. The designed protein is the optimization of the For N-terminal protease activity to achieve high catalytic efficiency for a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-   diphosphate + H(+) + phosphate. The designed protein sequence is ",
    "response#1": "ANKSGIYHLTNDCPNSSIVYEAEDVILHLPGCVPCVAANNATQCWVPASPTLAIPNASAPIRGFRGHVDLMVGAAVVCSAMYVGDLCGGAFLVGQLFAFRPRMHRTVQSCNCSIYTGRITGHRMAWDMMMNWSPTATWVVSYALRXPQLIIDIFAGGHWGVAAGLLYYSMVANWAKVIGILFLFAGVDTRVTTRLSDDECVRIEAKARPTLTVAVESLDITPSLRFTKELGLDFSVYSASENLGCKGGWTYNCAHLKGDQGVFSGLPAQDKAGLTARLECYSDVGWGEGCFIFGAAHLCTVVNFAPPKETLHCSEIQSEKVVANLEVSVGDSLQRKLRFSDWSSTALMDLEKFGKVRMDCVLKNKIQLANSYLCTYNKTNDLTHFLVPKTIVDAIDIPYRHPNSTWRDISKVVNFERCDGADLDLVGESAGQTAGGIAKIFDLGANQPIQVIYANGSGRVNRTALNCFDSIQTGFLAALFAKKVNSSACPQAMASCRDLSSFPQGWGPITYANITNPEGTPYCWHYAPRPCGIEPARNICGPVYCFTPSPIVIGTTTVDGTPTYNSGANATDVLLLASLRPPAGAWYGCTWMDNEGYVRTCGAPPCKIRPDSNVSDPAELQCPTDCFRKHPDATYGRCGSGPWLTPRCLVHYEYRLWHYPCTVNYTLHKVRMFVGGVEHRFQAACNWTRGAPCSLEHRDRTEISPLLFSTTELAILPCSFTPMPALSTGLIHLHQNIVDVQYLYGITSNFASWVIKWEYVVLLFLILAD",
    "response#2": "QVRNASGLYILTNDCANSSIIFEGDGIILHMPGCVPCEKKNETEFLCWAGVTPTLSVRDPGAPTAGFRRHVDLIAGAAVVCSSLYIGDICGGVFLVGQLFTFQPRRYWTTQECNCSIFLGHVSGHRMAWDMMQNWSPTVTWVISSLLRIPQLLFDMVSGGHWGVLAAVAFYGMSSNWLKVLVVMVLFAGVLFANTSGVYHLTNDCPNSSIVYEAETMILHLPGCVPCVRTGNASRCWVPATPTLAIPNETIPVQGFRRHVDLMVGTASFCSAMYIGDLCGAVFLVGQLVTFRPRYHTFAQDCNCSIYTGRITGHRMAWDMMMNWSPTVALITSSVLRVPVIIYDIFVGTHWGILAGLLYYGMVGNWAKVIVVLMLFAGVLAVGAQTARNTFALTSLFDSGPQQKLQLINTNGSWHINRTALNCNDSLNTGWLASLFYNNKFNSSGCPERLSACKPITHFPQGWGPITYENISGPSEDRPYCWHYAPRPCSVVSASTVCGPVYCFTPSPVVVGTTDRKGVPTYNWGENETDVFLLESLRPPKGGWYGCTWMNSTGFVKTCGAPPCNIGPPSNSTLKCPTDCFRKHPGATYAKCGSGPWLTPRCLVHYPYRLWHYPCTVNYTIHKVRLYLGGLEHRFEAACNWTRGDRCELDDRDRIEMSPLLHSTTELAILPCTFTTMPALSTGLIHLHQNIVDVQYLYGVSSSLTSWVIKWEYIVLAFLVLAD",
    "response#3": "RNLIIKHKVRNETVASWYGEEEVYGMPKIMTIIKASTLSKSRHCIICTVCEGREWKGGTCPKCGRHGKPITCGMSLADFEERHYKRIFIREGNFEGMCSRCQGKHRRFEMDREPKSARYCAECNRLHPAEEGDFWAESSMLGLKITYFALMDGKVYDITEWAGCQRVGISPDTHRVPCHISFGSRMPFRQEYNGFVQYTARGQLFLRNLPVLATKVKMLMVGNLGEEIGNLEHLGWILG"
  },
  {
    "reference": "MMSFVSLLVVGILFPAIQAKQFTKCELSQVLKDMDGYGDITLPEWICTIFHISGYDTKTIVHDNGSTEYGLFQINNKLWCRDNQIQSKNICGISCDKFLDDDLTDDMMCAKKILDNEGIDYWLAHKALCSEKLDQWLCEKM",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The presence of C-type lysozyme domains is critical for the interaction of the designed protein with its intended targets.2. The protein should have a high affinity for Ca(2+).3. For general function, the protein need meet that Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme; This enables LS to synthesize lactose, the major carbohydrate component of milk4. Design the protein with a signal peptide incorporated.5. The designed protein should be involved in lactose biosynthetic process by localizing to extracellular region and exhibiting lysozyme activity, lactose synthase activity, calcium ion binding. The designed protein sequence is ",
    "response#1": "PYVTTIFGGLHAGKMVMLQGVVPLDAHRFQVDFQCGCSLCPRPDIAFHFNPRFHTTKPHVICNTLHGGRWQREARWPHLALRRGSSFLILFLFGNEEVKVSVNGQHFLHFRYRLPLSHVDTLGIFGDILVEAPE",
    "response#2": "NMKSGMSLKIKGKIHNDVNSFTINLGQGKETLNLHFNPRFNESTIVCNTLDGSSWGQEQRENHSCFSPGSEVKLTLTFQDKDFKVTLPDGHSLTFPNRLGHNHLRYLSMDGLQISSFKK",
    "response#3": "VPYVTTIFGGLHAGKMVMLQGVVPLDAHRFQVDFQCGCSLCPRPDIAFHFNPRFHTTKPHVICNTLHGGRWQREARWPHLALRRGSSFLILFLFGNEEVKVSVNGQHFLHFRYRLPLSHVDTLGIFGDILVEAVGFQ"
  },
  {
    "reference": "MKIGVMGAGSWGTTLAKVFSDAGCDVTLWARREEVAREINTEHTNSTYLRGIALPHTLTATTQPTQALCDADVVVLAVPSQTLRGNLAEWCADIPQDALLLSLAKGIEKETFLRMSEVIAEVTGAQPDKVAVLSGPNLAREIAEEQPAATVIACTNEKNAQRIQHALAAPYFRPYTNTDVIGCEIGGACKNVIALACGMASGRGLGENTLATLMTRGLAEISRLGVAMGADPRTLSGLAGLGDLVATCSSPLSRNRTFGARLGEGKTLDEARAATNGQVAEGVISSQSIARLADSLGVDMPITRAVFGVCHRDQNVADMVAALMGRTKKSE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein with NAD binding functions is required to enable carbohydrate metabolic process, glycerophospholipid metabolic process, glycerol-3-phosphate biosynthetic process.2. The substrate binding site should be stable and able to withstand changes in environmental conditions.3. The protein should have a fast turnover rate for NADP(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate   + H(+) + NADPH.4. Ideal protein should have a binding affinity for key enzymes in the glycerophospholipid metabolism pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "DIIGVEIGGAVKNIIALAAGVCDGIGYGDNSKAALMTRGMAEIARIGIKMGGKAETFFGLTGMGDLIVTCTSMHSRNRRAGILIGQGKTAEEAIEEVGMVVEGIKACKAFYELKEKEGVTMPITDIAYKVLFEGAKAENALAKLK",
    "response#2": "DVIGAEFGAALKNIIAIGAGAIQGLGYHDNARAALITRGLAEIRRLGVAFGANPMTFIGLSGVGDLVVTATSKNSRNWRAGYQLGQGKKLQDVIDNMGMVIEGVYTTKAAYELSRKRQVQMPITEALYRVLYEGEDIKTAAFLVE",
    "response#3": "DMVGAELGGAMKNVLAVATGVADGMQLGLNARAGLITRGLNEMLRLAAVIGARPETLMGLAGLGDLVLTCTGDLSRNRRLGLALGRGQSLSDAIREIGQVVESVQTADEVMRQAEQHGIELPISNAVRAVLHGEITPENQ"
  },
  {
    "reference": "MCLKDWLCAFCCLRLGCLEYPKNEKQPLVSGNNNQYKTYAVIDDDERNLWSSPHDLSHTEAVDDRMLQSWIQTRNQLEKDSEEWQKLNYEIYTLRQARREVRARWRRILEDLGFQKEAETLLSVTKQSTISNPKNMKRAMELLVKLSDNTTIFPAGWDLPVRYLYIMDRLICLDAVDDFLNIAQKKYPKTKMDISQ",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be optimized for stability and solubility when expressed in melanosome membrane, organelle membrane, cytoplasmic vesicle membrane, lysosomal membrane where it will be involved in phagosome maturation.2. The protein must exhibit the following characteristics: Probably functions as cargo-recognition protein that couples cytoplasmic vesicles to the transport machinery; Contributes to retrograde melanosome transport from the cell periphery to the center; Binds phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate and phosphatidylinositol 3,5-bisphosphate.. The designed protein sequence is ",
    "response#1": "RKLLEMRGAFIIRQLCLLLHAENIFHSMADILLKEEDLKFASTMVQTLNTILLTSAELFQLRNQLKDLRTQESCALFCCLYRSWCHNPVATVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLMEVDKLVQLIESPIFTYLRLQLLDVEHNPYLIKALYGLLMLLPQSQAFQLLSHRLSCS",
    "response#2": "RKLLEMRGAFIIRQLCLLLHAENIFHSMADILLKEEDLKFASTMVQTLNTILLTSAELFQLRNQLKDLRTQESCALFCCLYRSWCHNPVATVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLMEVDKLVQLIESPIFTYLRLQLLDVEHNPYLIKALYGLLMLLPQSQAFQLLSHRLSCL",
    "response#3": "QVQRIYVGDGSITTSDEAKFTIWKVTTELIVHKVEPVRVSIPLEIVSYRRYSEFDQLRAEIISKIGKNVQVPELPPKVYWYDYWRFSKINLNRHWLNKRRQGLDNFINKIIKNKDIMESCAKEVNEFLGVSSDVR"
  },
  {
    "reference": "MIHNKTTGERKLMLFSGRAHPRLATEVAACLGLEPAPTQARDFANGEIYIRYDASARGADCFVLQSHTAPINQWLMEQLIMIDALKRASARSITVIAPFYPYARQDKKHLGREPISARLVADLLQRAGAERLMCVDLHAAQIQGFFDGPVDHLFALPVLAAHVAAHHSPDQLTIVSPDAGGVRRADQWSQHLGAPLAIVHKRRDPRTAHSVKAISVVGDIQDRTCVLVDDMVDTAGTICAAADALHDHGAREVIVAATHAVLSDPAPQRLRDSAVAEFLFTDTLPLPQSLAQAANVTTLPIAPLIARAIQEVFDDGSVTQLEGTSPPHPRRTETEQADGTRDGSSTHP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein with ATP binding, magnesium ion binding functions is required to enable nucleotide biosynthetic process. The designed protein sequence is ",
    "response#1": "SHKYERGHTLVASGGATRTGAARLAARAALRIGSGLVTVASPPEALGVNAAQLTAVMLRGCDGPEGLHAILQDPRFNALVLGPALGVHAATRAMVSVAVHARRSLVLDADALTSFEGLASELHGAFRHAPTVLTPHGGEFNRLFKGHPDILDPESKIERTRRAAAYTGAVVLLKGADTVIAAPDGRAAINENGTPYLATAGSGDTLCGLIAGLMAQGLPAFEAACAGAWIHAEAGAQFGPGLISEDLAEQVPAVLR",
    "response#2": "SHKYERGHTLVASGGATRTGAARLAARAALRIGSGLVTVASPPEALGVNAAQLTAVMLRGCDGPEGLHAILQDPRFNALVLGPALGVHAATRAMVSVAVHARRSLVLDADALTSFEGLASELHGAFRHAPTVLTPHGGEFNRLFKGHPDILDPESKIERTRRAAAYTGAVVLLKGADTVIAAPDGRAAINENGTPYLATAGSGDTLCGLIAGLMAQGLPAFEAACAGAWIHAEAGAQFGPGLISEDLAEQVPAVLP",
    "response#3": "HALPKPGLSSHKYERGHTLVASGGATRTGAARLAARAALRIGSGLVTVASPPEALGVNAAQLTAVMLRGCDGPEGLHAILQDPRFNALVLGPALGVHAATRAMVSVAVHARRSLVLDADALTSFEGLASELHGAFRHAPTVLTPHGGEFNRLFKGHPDILDPESKIERTRRAAAYTGAVVLLKGADTVIAAPDGRAAINENGTPYLATAGSGDTLCGLIAGLMAQGLPAFEAACAGAWIHAEAGAQFGPGLISEDLAEQVPAVV"
  },
  {
    "reference": "MNQNDDKNKKDPNEQDPFNFFKLSSEPSNNNNNKDQKKPRLPFIAIIAGVFIILLIVNQFLMKQDANIIPFSEFKDRVASGEIVKVIMGPTYFIGQTKTQASSEQTRSKLPFLPADAGDAYQTVGIYSESFLQLLDEHNVIYLVRPKENNFLVDFLVQWILPFGFIFLLWHFVMKRFTSNLGGLGGSIFSGGQARSAAVEEGKVTTRFSDVAGVDEAKEELVEVVDFLKYPQKYTEIGGKIPRGVLLVGPPGTGKTLLARAVAGESGVPFFRISGSDFVEMFVGVGASRVRDLFKQAREKAPCIIFIDELDAIGKSRLNSIHSNDEREQTLNQLLVEMDGFDNSTGLILLAATNRPDVLDPALLRPGRFDRQVAVDRPDMKGREQILKIHAKNVKLSSGIDLSDTARITSGFSGADLANVINEAALLAVRGGRKEVIAEDLNEAVEKAIAGLQKKSRVIKEKERQIVAYHETGHAITAAFTEGADKVHKVSIIPRGIAALGYTLNIPEEDRFLSTEKELLAEVDCLLGGRAAEFVQFGVVSTGASSDLSRATDIIRSMITDYGMSDRFKNVALSKRGGGYGAGDPQLVREYSETTQQYIDEEIARIMEERYAVVVNRLQEKKTLLEYIAKRLLEKETIDKQEFEDIIKAESQLPRQPILQSPEAALSDESDVSGKAGAFAADSSDSSAPQQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.4. The designed protein must have a active site that is structurally stable and resistant to denaturation.5. The designed protein must possess zinc ion binding, ATP hydrolysis activity, ATP-dependent peptidase activity, metalloendopeptidase activity, ATP binding to support protein catabolic process, cell division, proteolysis under certain conditions.6. For general function, the protein need meet that Plays a role in the quality control of integral membrane proteins.7. The designed protein should have a binding site that is specific to the target Zn(2+), ATP ligand. The designed protein sequence is ",
    "response#1": "MPKGVLLVGAPGTGKTLLARAVAGEAGVPFFNISGSEFIEMFVGVGAARVRDLFEQARQKAPCMIFIDELDAIGRARGGPVVMGGHDEREQTLNQLLTEMDGFDPSIGVVVMAATNRPEILDRALLRAGRFDRQIVVDKPGAADMLRVSAAAKGLDVDLEIIARGTPGFTGADLENLVNEAALLAARLDKDYVSMSDIDHAIARGDFTKAKDIQDWILTGK",
    "response#2": "MPKGVLLVGAPGTGKTLLARAVAGEAGVPFFNISGSEFIEMFVGVGAARVRDLFEQARQKAPCMIFIDELDAIGRARGGPVVMGGHDEREQTLNQLLTEMDGFDPSIGVVVMAATNRPEILDRALLRAGRFDRQIVVDKPGAAELLEILESQGRLDSLPEDERARVKAEIIARGTPGMAGADLANLVNEAALFAARAGKRLVDMDDFVRTLGGLPPFSIITQHEKRSIAIHEAGHATLSWFLEHANPLVKVTIVPRGKALGAAWYLPEERQITTKEQMLDEMCALLGGRAAEELFIGQISSGAMNDLERVTKQAYAMITYMGMSEKLPNLSYYDSTGQEYTFSKPYSEDTAKLIDSEVKAMIAQQYERAKQILSEHAEGHNKLAEILLEREIIYADDLEHRFLPQANGR",
    "response#3": "MVMSDKEKINTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTQYLPEEDRYSMSKRQIFGQLCSLFGGRIAEELVGGFEGVTTGAQNDIERATQMARNMVTKWGLTERMGPVLYGEDESQAPGGGNTHYSEDTSREIDEEVRIILDSAYVKSKALLEEHRDVLESMKDALIEYETIDAEQVDARAAAREQR"
  },
  {
    "reference": "MIEKDKRTIAFIHPDLGIGGAERLVVDAALGLQQQGHSVIIYTSHCDKSHCFEEVKNGQLKVEVYGDFLPTNFLGRFFIVFATIRQLYLVIQLILQKKVNAYQLIIIDQLSTCIPLLHIFSSATLMFYCHFPDQLLAQRAGLLKKIYRLPFDLIEQFSVSAADTVVVNSNFTKNTFHQTFKYLSNDPDVIYPCVDLSTIEIEDIDKKFFKTVFNEGDRFYLSINRFEKKKDVALAIKAFALSEDQINDNVKLVICGGYDERVAENVEYLKELQSLADEYELSHTTIYYQEIKRVSDLESFKTNNSKIIFLTSISSSLKELLLERTEMLLYTPAYEHFGIVPLEAMKLGKPVLAVNNGGPLETIKSYVAGENESSATGWLKPAVPIQWATAIDESRKILQNGSVNFERNGPLRVKKYFSREAMTQSFEENVEKVIWKEKKYYPWEIFGISFSNFILHMAFIKILPNNPWPFLFMATFMVLYFKNYLWGIYWAFVFALSYPYEEI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should contain a Helical membrane-spanning region.2. The protein should have a fast turnover rate for alpha-D-Man-(1->3)-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-   alpha-D-GlcNAc-diphosphodolichol + GDP-alpha-D-mannose = alpha-D-Man-   (1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-   alpha-D-GlcNAc-diphosphodolichol + GDP + H(+).3. The designed protein should have alpha-1,6-mannosyltransferase activity, glycolipid 1,6-alpha-mannosyltransferase activity, alpha-1,3-mannosyltransferase activity within endoplasmic reticulum membrane, endoplasmic reticulum to regulate protein glycosylation.4. The protein should be optimized for activity in protein glycosylation, Protein modification pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "FLTVVAFCVRAQRLMNPAKVVFEELRYYNYAVDYVNNKLLMDVYPPLGKLLFSLVAALTGNKYELNTLDEPGQQYPFTDVAYSMRLFTCLLGSLLVPLMYGTVYFPTKSKTAASLAALFVIFDNGLITMSRYIMIEIPALYFMSLTAFYWSVYEAQQKRPFSLRWHTSLLSTGVALGLALSTKLSAMFTFGWLLILAAFHLWNLLGDLSVPMYRIVKHLFSYIFYLIGVPITVYLAVFAVHSHILYRRPNTGVHLVKVVAFFHPYCNAGGGGERVLWCALRSLQKRYKDAIYVIYTGDKDASEEQILNGAAARFNIKLSHPVRFIFLEKRGLVEASCYPRFTLLGQSLGSVVLGWEALTKCVPDIYIDSMGYAFTLPLFKYLGGCHVGCYVHYPTISMDMLSVVRSQHARFNNAAFISNNPVLSRLKLIYYYLFALFYGWVGSCSDVIMVNSTWTFSHILDLRERPALLVSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKGPLREYYSRLIHQKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAVNFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFGHLLVSIGQFRPEKNHALQIKAFAKLLNEKAAELGHSLKLVLIGGCRNKDDEFRVNQLRSLSENLGVQENVEFKINISFDELKNYLSEATIGLHTMWNEHFGIGVVECMAAGTVILAHNSGGPKLDIVIPHEGQITGFLAESEEGYADSMAHILSLSAEERLQIRKNAR",
    "response#2": "FLTVVAFCVRAQRLMNPAKVVFEELRYYNYAVDYVNNKLLMDVYPPLGKLLFSLVAALTGNKYELNTLDEPGQQYPFTDVAYSMRLFTCLLGSLLVPLMYGTVYFPTKSKTAASLAALFVIFDNGLITMSRYIMIEIPALYFMSLTAFYWSVYEAQQKRPFSLRWHTSLLSTGVALGLALSTKLSAMFTFGWLLILAAFHLWNLLGDLSVPMYRIVKHLFSYIFYLIGVPITVYLAVFAVHSHIRRNYYWRSFVGDLVPKGKKFLLLSINRYERKKNLTLALEALVQLRGRLTSQDWERVHLIVAGGYDERVLENVEHYQELKKMVQQSDLGQYVTFLRSFSDKQKISLLHSCTCVLYTPSNEHFGIVPLEAMYMQCPVIAVNSGGPLESIDHSVTGFLCEPDPVHFSEAIEKFIREPSLKATMGLAGRFLESFVEHNKLMWLKDRKMGDGHVYESSALTWPLLLGPLRFFYEQHLQVFFMGNPFVWYSVISLVAFFVIVQIFCLARWNLGYNDFGPSAFHYNYNIGKFVVAWLLHWAPYILETDRVFLYHYLPALYFGIAALGVSWSFLGNAVFGNRTAYKALSVIIMALMFLVYRLYSPFTYMTTLTKSSCRALELKGSS",
    "response#3": "FLTVVAFCVRAQRLMNPAKVVFEELRYYNYAVDYVNNKLLMDVYPPLGKLLFSLVAALTGNKYELNTLDEPGQQYPFTDVAYSMRLFTCLLGSLLVPLMYGTVYFPTKSKTAASLAALFVIFDNGLITMSRYIMIEIPALYFMSLTAFYWSVYEAQQKRPFSLRWHTSLLSTGVALGLALSTKLSAMFTFGWLLILAAFHLWNLLGDLSVPMYRIVKHLFSYIFYLIGVPITVYLAVFAVHSHILWYFSFIFLSLYSFLTVVAFCVRAQRLMNPAKVVFEELRYYNYAVDYVNNKLLMDVYPPLGKLLFSLVAALTGNKYELNTLDEPGQQYPFTDVAYSMRLFTCLLGSLLVPLMYGTVYFPTKSKTAASLAALFVIFDNGLITMSRYIMIEIPALYFMSLTAFYWSVYEAQQKRPFSLRWHTSLLSTGVALGLALSTKLSAMFTFGWLLILAAFHLWNLLGDLSVPMYRIVKHLFSYIFYLIGVPITVYLAVFAVHSHIRERPALLVSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKGPLREYYSRLIHQKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAVNFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFFLESFVEHNKLMWLKDRKMGDGHVYESSALTWPLLLGPLRFFYEQHLQVFFMGNPFVWYSVISLVAFFVIVQIFCLARWNLGYNDFGPSAFHYNYNIGKFVVAWLLHWAPYILETDRVFLYHYLPALYFGIAALGVSWSFLGNAVFGNRTAYKALSVIIMALMFLVYRLYSPFTYMTTLTKSSCRALELKGS"
  },
  {
    "reference": "MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPKPGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDADRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPFFDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRFFSYGDAMFITYNPQAINERVGE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a low Km value for 7-aminomethyl-7-carbaguanosine(34) in tRNA + S-adenosyl-L-   methionine = adenine + epoxyqueuosine(34) in tRNA + H(+) + L-   methionine.2. A protein should be able to manipulate the activity of enzymes in the tRNA modification pathway, either by activating or inhibiting their function.3. The protein should be optimized for stability and solubility when expressed in cytosol where it will be involved in response to radiation, queuosine biosynthetic process. The designed protein sequence is ",
    "response#1": "MSNRYAVVTGANKGIGFETARQLGERGMTVYAGVRDVARGEAAVAELSDRVRPLELDVTSPESIDAAAAQVGERFDGLDVLVNNAGVALSGDSTPFEQASPASVAEQLDVNFFGVVRMVQAFAPLLRRSAGRVVNVTSGGGLAALTAQSPAYRATKSALNALTVQWATELAPYGITVNAVAPGYIATDLTRDVEAARGGRIVEMLEAGIPLRRWGRPEDVAGVVSFLVSEDSGYITGQTINVDGGMLVN",
    "response#2": "MPIGVPKVLFRNPGDPISSVW",
    "response#3": "MLKFENLVVLAGPTGSGKTALSIELAKRFNGEVINA"
  },
  {
    "reference": "MRVRGIRRNCQCWWGWSNMMLLGILMICSVTGNLWVTVYYGVPVWKEANTTLFCASDAKGQDPEKHNVWATHACVPTDPNPQEVLLGDNVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDLKNSTTNNSTATNNTTNNSTEKMEMDKGEMKNCSFNITTNIRDKMQKEYALLYKLDIIPIDNGKNNSNTNYRLISCNTSVITQSCPKVSFQPIPIHYCAPAGFAILKCNNKTFNGSGPCTNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSANFSDNTKTIIVQLKEAVEINCTRPNNNTRKSINIGPGRAFYATGNIIGDIRQAHCNISGIKWNKTLAEIAKKLSKQYENRTIAFNQSSGGDLEIVMHSFNCGGEFFYCNTTQLFRDTWNYTWNGTWNNGTEVNRTITLPCRIKQIINMWQEVGKAMYAPPIRGQINCSSNITGLLLTRDGGESNKTIEVFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGLGALFVGFLGAAGSTMGAASITLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGVKQLQARVLAVERYLKDQQLLGLWGCSGKLICTTAVPWNTSWSNKSSDYIWNNMTWMQWEREIDNYTDVIYKLIEESQNQQEKNEQELLALDKWANLWNWFDITKWLWYIKIFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSLQTHFPAPRGPDRPEGTEEEGGDRDRDRSTPLVDGFLAIIW",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The ideal protein would be one that plays a critical role in actin filament reorganization, fusion of virus membrane with host plasma membrane, positive regulation of plasma membrane raft polarization, positive regulation of receptor clustering through structural molecule activity within host cell plasma membrane, host cell endosome membrane, virion membrane, viral envelope.2. Incorporating a YXXL motif; contains endocytosis signal motif is critical for the successful design of the protein.3. The designed protein have Retroviral envelope protein GP41-like domains with specific sequence motifs that are crucial for its activity.4. Ensure the presence of a signal peptide within the protein.5. The designed protein should contain Cytoplasmic topological domains.6. For general function, the protein need meet that The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane7. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "MRVRGTQRNWPNLWTWGTLILGLVIMCRAAENLWVTVYYGVPVWKDADTTLFCASDAKAYETEKHNVWATHACVPTDPNPQEINLENVTEEFNMWRNSMVEQMHSDIISLWDQSLKPCVQLTPLCVTLNCSSNVTVTNNNLWVTVYYGVPVWKEAKTTLFCASDAKAYVKEVHNIWATHACVPTDPNPQELVLENVTENFNMWKNDMVEQMHEDVISLWDQSLKPCVKLTPLCVTLNCTMLNNGTNNNETHAYNRRGEMRNCSFNATTEVRDRKQKVFALFYRSDIVPLNNSTLAENNSSQSSYSNYRLIKCDTSTITQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGTGPCQNVSTVQCTHGIMPVVSTQLLLNGSLAEGEIIIRSENLTDNVKTIIVQLNKSVEIVCTRPGNNTRKSIRIGPGQVFYGTGDIIGDIRQAHCNINRTDWNETLHKVSKKLRKHFPHDNITFAPSSGGDPEITTHSFNCGGEFFYCNTSGLFNGTYSNSTYSNSSSSSNTTIITIPCRIKQFINMWQEVGRAMYAPPIAGNITCTSNITGLLLVRDGGTNGINKXDNEIFRPGGGDMRDNWRSELYKYKVVEIKPLGVAPTGAKRRVVEREKRAVGIGAMIFGFLGAAGSTMGAAAVTLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTAVPWNTSWSNKSLNEIWNNMTWMEWEKEIDNYTNLIYNLLEESQNQQEKNEQELLELDKWANLWNWFDISNWLWYIKIFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQ",
    "response#2": "MRVMGIRRNCPLLWRWGMIIFWIMIICNAEKLWVTVYYGVPVWRDTETTLFCASDAKAYKAEAHNVWATHACVPTDPNPQEIYLKNVTENFNIWKNNMVDQMHTDIISLWDQSLKPCVKLTPLCVTLNCSNYNGTTDTGMKNCSFNITTEIRDKRRKEHALFYKLDIVPLSNNSREYILINCNTSVITQACPKVSFDPIPIHYCAPAGYAILKCNNKTFNGKGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRTENLIDNTKTIIVHLNESVNIVCIRPNNNTRKSVGIGPGQTFFATGEIIGDIRKAYCNISKDAWNKTLEKVKGKLKEHYHKNITFKQPVGGDLEITMHSFNCRGEFFYCNTSGLFNITQNHTYIIIPCKIKQIINMWQGVGRAIYAPPIAGNITCNSTITGLLLTWDGGNKTNETEEIFRPGGGNMKDNWRSELYKYKVVEIKPLGIAPTKAKRRVVEREKRAVGIGAVFIGFLGAAGSTMGAASVALTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLALERYLQDQQLLGIWGCSGKLICTTTVPWNSSWSNKTHDEIWKNMTWMEWDREISNYTDLIYGLLQDSQNQQERNEKELLALDRWKDLWSWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSLQTLTHHQREPDRPEGIEEEGGEQDRGRSVRLVNGFLALAWDDL",
    "response#3": "MRVKGIQMTWPNLWSWMILFLGVWMICNANNLWVTVYYGVPVWKEAKTTLFCASDAKAYESEVHNVWATHACVPTDPNPQEMVLENVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNVSTDTGMKNCSFNITTEIRDKRRKEHALFYKLDIVPLSNNSREYILINCNTSVITQACPKVSFDPIPIHYCAPAGYAILKCNNKTFNGKGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRTENLIDNTKTIIVHLNESVNIVCIRPNNNTRKSVGIGPGQTFFATGEIIGDIRKAYCNISKDAWNKTLEKVKGKLKEHYHKNITFKQPVGGDLEITMHSFNCRGEFFYCNTSGLFNITQNHTYIIIPCKIKQIINMWQGVGRAIYAPPIAGNITCNSTITGLLLTWDGGNKTNETEEIFRPGGGNMKDNWRSELYKYKVVEIKPLGIAPTKAKRRVVEREKRAVGLGAVFLGFLGAAGSTMGAASMALTVQARQLLAGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLGDQQLLGIWGCSGKLICTTAVPWNISWSNKSVDYIWNNMTWMEWEREIDNYTGTIYTLLEESQKQQEKNEKDLLELDQWASLWNWFDITRWLWYIKIFIMIVGGLIGLRIVFAVLSIVNRVRQGYSPLSLQIHHPPPGGPDRLGRIEEEGGEQDKDRSIRL"
  },
  {
    "reference": "MEFPFDINQLFSERVSVLDQTLVGGRKSAGRPDLQAHIATVIDELGRASAKAQHLPAPITSASKMQSQRHQLYLLKDGESNGGRGVAVGFLKVGYKKLFLLDLQGVHIEVEPLCVLDFYIAENLQRHGYGMELFDFMLQHKNLEPVLMAYDRPSPKFLSFLAKHYCLTQSVPQVNNFVVFEGFFFNRSAAQLRKVPLRKPDGEIKPYSLMEREGVRQEQRTLPWPFAPPYSPQRSVSSQYSHSLSVGSSPSRAPTRAAPASAPGGSNRDQSPQSPHIDRCRARRTSQQGLVARCSMYSRHMDSRAVGLLDRNLAGLGSMGDHSKNELRYTDTHRLASIHTPQNRSHPPPSLPPLSPSKKDNICSQTSVDGAETHLDADAETLGAKEEIKEVSGSPQRQAGSTAINRRPVLLGKVSDRSRGDWSWTVGENCYTAQWVKQKQEYRSTRPW",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: May be involved in neuron development.. The designed protein sequence is ",
    "response#1": "CEKACNPRMGNLALGRKLWADTTCGQNATELYCFYSENTDLTCRQPKCDKCNAAHPHLAHLPSAMADSSFRFPRTWWQSAEDVHREKIQLDLEAEFYFTHLIMMFKSPRPAAMVLDRSQDFGKTWKPYKYFATNCSATFGLEDDVVKKGAICTSKYSSPFPCTGGEVIFKALSPPYDTENPYSAKVQEQLKITNLRVQLLKRQSCPCQRNDLNDEPQHFTHYAIYDFIVKGC",
    "response#2": "CEKACNPRMGNLALGRKLWADTTCGQNATELYCFYSENTDLTCRQPKCDKCNAAHPHLAHLPSAMADSSFRFPRTWWQSAEDVHREKIQLDLEAEFYFTHLIMMFKSPRPAAMVLDRSQDFGKTWKPYKYFATNCSATFGLEDDVVKKGAICTSKYSSPFPCTGGEVIFKALSPPYDTENPYSAKVQEQLKITNLRVQLLKRQSCPCQRNDLNDEPQHFTHYAIYDFIVKGC",
    "response#3": "CEKACNPRMGNLALGRKLWADTTCGQNATELYCFYSENTDLTCRQPKCDKCNAAHPHLAHLPSAMADSSFRFPRTWWQSAEDVHREKIQLDLEAEFYFTHLIMMFKSPRPAAMVLDRSQDFGKTWKPYKYFATNCSATFGLEDDVVKKGAICTSKYSSPFPCTGGEVIFKALSPPYDTENPYSAKVQEQLKITNLRVQLLKRQSCPCQRNDLNDEPQHFTHYAIYDFIVKGQ"
  },
  {
    "reference": "MKLQHVVYLVAIFFVVAIFVIACIEENKYLVWIMIILANTTNILSLVRSISYIKNIRKHQKDTKIQRQLFFEKNGGGMLIERLSGAGSSNIDFKIFTEEDMKEATNGYDVSRILGQGGQWTVYKGILPDNSIVAIKKTRLGDNNQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYEFITGGSLFDHLHGSMFVSSLTWEHRLEIAIEVAGAIAYLHSGASIPIIHRDIKTENILLDENLTAKVADFGASKLKPMDKEQLTTMVQGTLGYLDPEYYTTWLLNEKSDVYSFGVVLMELISGQKALCFERPETSKHLVSYFVLATKENRLHEIIDDQVLNEENQREIHEAARVAVECTRLKGEERPRMIEVAAELETLRAKTTKHNWLDQYPEENVHLLGSNIVSAQGHTSSRGYDNNKNVARFDIEAGR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be able to regulate cell surface receptor signaling pathway. The designed protein sequence is ",
    "response#1": "LELTREVYTSLDHLTRPPGLFTWLSDIELYTESVAEGDYLSGIRESKYYTGNQFFTMKNIYGNTNRLSKQLITLLPGEFMTHLSINRPFQTIAGINKLYSLIQKIVFTTFKNDNEYQKNFNVNNQNEPQETTNYPNDYGGSNSQKFKHNLSHFPLIIHKLEFAEYFHSIFALGWTHNSC",
    "response#2": "HHHHHKLLLIKKWRYRFSEHNGGNFCRDIAISSIVSGHKNFMQLVGCCLESEHPVLVYRASKKPTSLDLKMVVSWRQRLKIAEEIATALAYLHTAFPRPFVYRILRLEDILLDDEDGVAKLCNFSYCVSIPQGETFVKLGNGCIGGDYDYMDDNYLINGIVSEKTDAFGFGIFMQKLLMGEERFHELCYDRSDLTTFENRRKFAKC",
    "response#3": "LELTREVYTSLDHLTRPPGLFTWLSDIELYTESVAEGDYLSGIRESKYYTGNQFFTMKNIYGNTNRLSKQLITLLPGEFMTHLSINRPFQTIAGINKLYSLIQKIVFTTFKNDNEYQKNFNVNNQNEPQETTNYPNDYGGSNSQKFKHNLSHFPLIIHKLEFAEYFHSIFALGWTHNSC"
  },
  {
    "reference": "MGSPRWKRFCFLLLAAFTSSLLLYGHYYATVDVRSGPRVVTSLLQPELLFLVRPDTPHPDNSHHKELRGTVKSREFFSQPSSELEKPKPSGKQPTPCPRSVAATAKADPTFGELFQFDIPVLMWDQHFNPETWDRLKARRVPYGWQGLSQAAVGSTLRLLNTSSNTRLFDRHLFPGGCIRCAVVGNGGILNGSRQGRAIDAHDLVFRLNGAITKGFEEDVGSKVSFYGFTVNTMKNSLIAYEAYGFTRTPQGKDLKYIFIPSDARDYIMLRSAIQGSPVPEGLDKGDEPQKYFGLEASAEKFKLLHPDFLHYLTTRFLRSELLDMQYGHLYMPSTGALMLLTALHTCDQVSAYGFITANYEQFSDHYYEPEKKPLVFYANHDMLLEAELWRSLHRAGIMELYQR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Catalyzes the transfer of N-acetylneuraminyl groups onto glycan chains in glycoproteins2. The designed protein should have a specific function within the Golgi membrane. The designed protein sequence is ",
    "response#1": "HNFTVYVYDLPKEFNIGLLQNCRHLNIYTNMCPHVANNGLGQPLHRGRTSWFSTHQFIAEMIFHARVENHPCRTYEPDTADIFYVPFYGGLYASSVFREQNLTKRDELAVRLVNYISGQRWWKRSNGRDHFLAIGRTAWDFMRSSDTDFGANMLMQMPRVMNMSVLTVERQPWNGDNHFGIPYPSYFHPYTSAEMVTWQDKMKNVERPNLFSFVGGPRKGLEKAAIRDELIKQCAESSHCELLKCENGGSRCHNPMTVLGVMARSRFCLQAPGDSFTRRSTFDAMLAGCIPVFFSPHTMYTQYMWYLPDDKRSYSVFMDEKNNEGEIRHVASNLCIDTQFKEQNQRFGLRKCASEDKDGGGEQDLRLTRWHDIRPKGRKICFDVSTSVDKAPIILFDCHSMKGNQLFKYRMPQKQIYHPVSGQCLTADENGKGFLHMKKCDSSSKLQQC",
    "response#2": "LVLSLPSKVFSLTSKDQLKVEVTTVFGSAAPPLRVNLVQVLGSDSKVITTETKELQFDLDNNVHYLDIAPLKIDVGKYSLVFEISLQEQEHETIYATGGTNTEAIFC",
    "response#3": "LKLQIPCGLTQGSSITVIGIPDGLVGSFRIDLTGQPLPGEPDPPIIVHYNVRLLGDKSTEDPVIVQNSWTASQDWGAEERCPKFDPDMNKKVDDLDECNKMVGGEINRTSSTSLQSNTSRGVPVAREASKHEKYFPFKQGFLSVATLRVGTEGMQMTVDGKHITSFAFRDTLEPWLVSEIRITGDFRLISILAK"
  },
  {
    "reference": "MTIFEKKPDFTLFLQTLSWEIDDQVGIEVRNELLREVGRGMGTRIMPPPCQTVDKLQIELNALLALIGWGTVTLELLSEDQSLRIVHENLPQVGSAGEPSGTWLAPVLEGLYGRWVTSQAGAFGDYVVTRDVDAEDLNAVPRQTIIMYMRVRSSAT",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design must be able to perform identical protein binding in order to contribute to cellulose biosynthetic process. The designed protein sequence is ",
    "response#1": "MKWDATEPQQGVFTFSGGDQIATLAKTNGMLLRGHNCVWYNQLPSWVSSGSFTAAQLTSIIQNHCSTLVTHYKGQVYAWDVVNEPFNDDGTWRTDVFYNTLGTSYVQIALEAARAADPNAKLYINEYNIEFAGAKATSLLNLVKSLKAADVPLDGIGFQCHLIVGEFSGPGLQTQLSTFAAQGVEVAITELDIRMTLPSTPALLAQQQTDYNSVITACMNVESCIGVTVWDWTDKYSWVPNTFSGQGAACPWDQNFVKKPAFNGIAAGCA",
    "response#2": "AQNVVYWGQNGGGTIENNDLAAYCQPNSGIDVLVLAFLYQFGNGGNIPSGTIGQSCYISTSGQGQNCEALTAAIHTCQSAGVKIILSLGGATSSYSLQTQAQAEQIGQYLWDSYGNSGNKTVQRPFGSNFVNGFDFDIEVNGGSSQYYQYMIAKLRSNFASDKSNTYLITGAPQCPIPEPNMGVIISNSVFDHLYVQFYNNNNYTVPCALGINGNAPFNYNNWTSFIADTPSAGAKIFIGVPASPLASTGTPSGAQYYAAPDQLAAIVGEYRSDAHFGGIMMWSAGFSDANVNNGCTYAQQAKCF",
    "response#3": "MKWDATEPQQGVFTFSGGDQIATLAKTNGMLLRGHNCVWYNQLPSWVSSGSFTAAQLTSIIQNHCSTLVTHYKGQVYAWDVVNEPFNDDGTWRTDVFYNTLGTSYVQIALEAARAADPNAKLYINEYNIEFAGAKATSLLNLVKSLKAADVPLDGIGFQCHLIVGEFSGPGLQTQLSTFAAQGVEVAITELDIRMTLPSTPALLAQQQTDYNSVITACMNVESCIGVTVWDWTDKYSWVPNTFSGQGAACPWDQNFVKKPAFNGIAAGC"
  },
  {
    "reference": "MATSSVAAESEVPLWLRNLPLAPEYHPTLEEFQDPISYILKIEKDASKYGICKIIPPISSPPKKTAITQLNRSLSARNPNSVSKTLPTFTTRHQQIGFCPRKPRPVQKPVWQSGEAYTVQQFEAKAKAFERSYLKKSAKKKVFSELEIEALFWKASRDKPFTVEYANDMPGSAFVPINEKKWRESGIPTTVGETVWNMRGVSRHKESLLRFMKEDIPGVTSPMVYLAMLFSWFAWHVEDHDLHSLNYLHMGAGKTWYGVPKDAAVAFEEVIRVHGYGGEVNPLVTFATLARGLQCCLLKYLLVGFLAAIVQNVGELLSLSQELTIQDSAMVSPSLHILSFILELQNIYSMIQFGEAANIATPEWLTVANEAAIRRASINCPPMVSHLQLLYTLSLSLCPRFVFIGITDIYANPNTFSLVVYNDENLTRVHLCFSLCSVPRNISSDEPRSSRLKNKKRSEGQTMIKELFVQNMIQNNNLIHLLIEQGASCVLLPQSSLETFECSNLGVGSQVKVKPRLSLGLCTNKESLEASKTSLSNMDRNMGVRPYNESRLIEQGLFTCVTCGVLTFGCAAVIQPSDVAARYLRSADCGFFNEWAVGSGDRYPVAEEVDDTPEADHPSGQTEKSDHDGLHNVDLVPERVKSALDTGTNKDVTSLDLLAFAYGNSSDSEDDNANDRGDCSLFHQHSEFLSNGFGSHSLEHDSVDATVESSSYLSRRQGYGTDRFIKTYQRTKVSDRKQKQRSHDADIKGVEAPHIFSGNNTRELVHGE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must have at least one JmjC, JmjN, C2H2-type domain.2. The designed protein should be involved in positive regulation of lateral root development, histone H3-K9 demethylation, negative regulation of histone acetylation, regulation of photoperiodism, flowering, protein localization to heterochromatin by localizing to nucleus and exhibiting histone H3K27me2/H3K27me3 demethylase activity, sequence-specific DNA binding.3. Prioritize Basic residues, Basic and acidic residues, Acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "NSPWNLQAIARAPGSLTRFMPDDVPGVTSPMVYIGMLFSWFAWHVEDHDLHSLNFLHTGAPKTWYAVPGDRAVELEEVIRVHGYGGNTDRIASLAVLGEKTTLMSPEVLIDNGVPCCRLVQYPGEFVVTFPRAYHVGFSHGFNCGEAANFATPQWLKFAKEAAVRRR",
    "response#2": "YCLMGVKDSYTDFHIDFGGTSVWYHVLRGEKIFYLIRPTAANLSLFERWSSSSNQNELFFGDQVDMCYKCSVKQGNTLFIPTGWIHAVLTPVDCLAFGGNC",
    "response#3": "LPPRIASSEKKRTVFGSRNKKDKHGNCRCDYDLTEQTKIRLLRQNIVRLVAQEENSLRLYYYVDNALEYCKYEANFMEIDRVEANAIKMLINSYPKYVSISSLPLPNVEHCLDTATGLWERGLLITEEPC"
  },
  {
    "reference": "MQFDLLKTDGVARRGTLTLAHGVIQTPVFMPVGTYGTVKAMTPQSLNDIGAQICLGNTFHLWLRPGLEVIAAHKGLHDFMNWQKPILTDSGGFQVFSLGAMRKITEEGVKFSSPHDGAKLFLTPEISMQIQKVLNSDIVMIFDECTPYPATHEEAAKSMRMSMRWAQRSRDEHNKLENSNALFGIVQGGMHEDLRDESVAGLCDIGFDGMAIGGLSVGEPKEDMARILAHTAPQLPTHKPRYLMGVGTPEDLVYSVSAGIDMFDCVMPTRNARNGHLFTRFGDVKIKNARYKLDTGPLDSSCSCYTCTNFTRAYLHHLFRNGEILGGMLNTIHNLHFYQTIMAEMRTAIEVEHFTEWAAGFARDRSSGQ",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a low Km value for 7-aminomethyl-7-carbaguanine + guanosine(34) in tRNA = 7-   aminomethyl-7-carbaguanosine(34) in tRNA + guanine.2. The protein must be able to metal ion binding. The designed protein sequence is ",
    "response#1": "STNTAGRVGRLSTPHGILSTPQFMPVGTLGTVKGITATQLKDTNAQMILANTFHLHLQPGEAIIKESGGLHSFMSWDQPILTDSGGYQVFSLGKLNKIDDFGVSFKSPRDGSHIELTPEKAIQIQMDLGADVVMAFDQCPPYPASKVEVEEACKRTHLWLERCVATHSKEDQALFGIVQGGCFLDLREESARRVASFHLPGIAIGGVSVGEPSDQIHKIVRHVAPLLPNEVPRYLMGIGTIREMAVAVANGVDFFDCVLPTRLGRHGTALVRDERWNLRNACFRNDYQPLDTTCVCETCTNYNRAYLHHLIRNDELLGLTLLSLHNLSHLIRFSRAMAVAIEDDCFSEDFAPS",
    "response#2": "GRVGRLSTPHGILSTPQFMPVGTLGTVKGITATQLKDTNAQMILANTFHLHLQPGEAIIKESGGLHSFMSWDQPILTDSGGYQVFSLGKLNKIDDFGVSFKSPRDGSHIELTPEKAIQIQMDLGADVVMAFDQCPPYPASKVEVEEACKRTHLWLERCVATHSKEDQALFGIVQGGCFLDLREESARRVASFHLPGIAIGGVSVGEPSDQIHKIVRHVAPLLPNEVPRYLMGIGTIREMAVAVANGVDFFDCVLPTRLGRHGTALVRDERWNLRNACFRNDYQPLDTTCVCETCTNYNRAYLHHLIRNDELLGLTLLSLHNLSHLIRFSRAMAVAIEDDCFSEDFAPL",
    "response#3": "GRLGRLEVNGRRLKTPALLPVVNPNKPTLDPREISKLGFDGVITNAYIIRKHEHLREQALEEGVHGLLGFDGFVMTDSGSFQLAEYGDVEVSNEEIVRFQAKIGSDVGTILDVPTPPDAPRSRVERDLETTLKRAREAVELDEHPPLALTVQGSTYEDLRRLCAEKLAELPAAVYPVGGVVPLLEEYRFVDVVRVVLAAKSSLPPHRPVHLFGCGHPLAIPLAVAMGCDLFDSASYAIYARSDRYMSILGTLKLEELETFPCSCPACTRHDPDDVREMEPRERTRVLATHNLYELRRVIETTRQAIVSGELWELAESVCRAHSTNTAGRVGRLSTPHGILSTPQFMPVGTLGTVKGITATQLKDTNAQMILANTFHLHLQPGEAIIKESGGLHSFMSWDQPILTDSGGYQVFSLGKLNKIDDFGVSFKSPRDGSHIELTPEKAIQIQMDLGADVVMAFDQCPPYPASKVEVEEACKRTHLWLERCVATHSKEDQALFGIVQGGCFLDLREESARRVASFHLPGIAIGGVSVGEPSDQIHKIVRHVAPLLPNEVPRYLMGIGTIREMAVAVANGVDFFDCVLPTRLGRHGTALVRDERWNLRNACFRNDYQPLDTTCVCETCTNYNRAYLHHLIRNDELLGLTLLSLHNLSHLIRFSRAMAVAIEDDCFSEDFAPE"
  },
  {
    "reference": "MIEFYKVTSQELEFWLKAEGEPLFRKKQIFDWVYQRSVTDWDLMTNLSTELRQKLKSNFRLSTMELVRESESDDGETTKFLWRLREGTYVESVLIRSDKRRTVCVSSQVGCPARCAFCASGQKGFVRNLTSFEIVEQIIQINQYLAEQGERVTHVVYMGMGEPLKNYNAVVASIRQISHEEMLNLSQRRITVSTVGIIEGIRRLTKENMQVNLVFSLHAPNQQVRKKIIPYARKYPLDEIIDGIEEYARHTKRNATYEYILIEGINDELKHADELCELLKGKQCAVNLIPYNPVPGVRLKRPKTKAIKHFMYILRKNRLTVTCRYTKGDDIAAACGQLALQEEIECDQKAS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must be able to perform tRNA binding under specific conditions.2. The designed protein should contain one or more Radical SAM core domains that are essential for its function.3. The presence of [4Fe-4S] cluster is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "HPNRITACLSTQVGCPLKCRFCATGLSGYVRNLSVHEIIGQVLGMEIEAKVGVSNVVYMGMGEPLLNYENTMKSVRILIDKKLKGMSPRRITISTAGIVPRIYELIDENIDFVLSVSLHAVTNEKRSYLMPVNDRYPLEEVIQAIKDYIERTNRRVTVEYILIKNVNDDPRDAGKLGQLLAGIKVNVNLIPYNPVGIENFKRPDEGRIRRFLEILKNKGIG",
    "response#2": "SSQAGCTVACPFCSTGYQGFNRNLTTAEIIGQLWYARRAVEAERAGARIGEASAEAPDRVISNVVMMGMGEPLLNYDQVLGALRLMLDDNAYGLSRRRVTVSTSGVVPMMDRLSRDCPVALAVSLHAPNDTLRDRLVPLNRKHPLAELLDACNRYLEHAPRDFITFEYIMLDGINDADEHH",
    "response#3": "MAFHVMIIPSMNCPSDCSYCWGVDRDSRVMDMETVREMVSWLMEFRNEPATFTFHGGEPLLAGYEFYRDTLKLISERLSFLKPAFAIQTNLWLMTDEMAELFAEYSIPIGSSLDGPREINDYQRGDGYFDRTMQGYEIARKHGLRVSFISTFTSYSIRRREEIFNFFLENGMNLKLHPALPSLKSSDPEEWAITAEEYGDLLLYLLDSYLA"
  },
  {
    "reference": "MSLELYNTTRRDIPEQKLMQVIEGVLGAEGLVIEDLTAVYCGNRLSRRINCEFLQHDYPTDTITFRYNDGEAVEGELYISLDIVAENAQRFNVSFTDELFRVTIHSALHLAGFEDTSVEERASMKDKEDHYLNCFCQEDSL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Zn(2+) is required for the proper folding and stability of the protein.2. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.3. For general function, the protein need meet that Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA.4. A protein that contributes to rRNA processing by functioning in cytoplasm with zinc ion binding, endoribonuclease activity, metalloendopeptidase activity. The designed protein sequence is ",
    "response#1": "MVER",
    "response#2": "MWIG",
    "response#3": "MK"
  },
  {
    "reference": "MDAHLAFRGVGGPHLHLGVCGSVAAYRALDLVRQWQDAGVSVSATLTPSAQKFVTPLTFAALGAAPVYTAPFDDPQAPSPFAHLEPGQVAQALVIAPASAATIARLAQGQADELLACQALAFRGKAVIAPAMNPAMWSHPATQANIATLRERGCAVVEPGCGRTACGEEGQGRLADLREIYLAGLRALAPQDMEGIRVMVTLGPTREHWDGLRFWTNPSTGTMGAAVAVAAWLRGARVEAVCGPGTPWLPAGIARHNVGSARHMLEAAASVWPQCDAGVFTAAVADFSPVPPAQGGDKKFKKSDAPDGFDVHFAPNPDILKTLAADRRPEQKVVGFAAESQNLEDSVRGKLVSKKADMIVGNLLQDGFGTADNTVFVADASGREERWAHLSKTDVAWRLLSWLLSR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal domain.2. The protein must be able to phosphopantothenoylcysteine decarboxylase activity. The designed protein sequence is ",
    "response#1": "MRILLGITGGIAAYKAANLIRGFSELGHEVTAVPTENALRFIGKPTLEALSGHPVETDMYVDVDMVRHVELGQQADLIVIAPATASFIARLAAGIADDLLLNAVLASKAPVVLAPAMHTEMWQNSATRANIETLAQRGIRVLEPSSGRLTGADSGPGRLPETNEIISQAEKFFGS",
    "response#2": "AGKKVMLTMGPTREFWDGIRFWSNPSTGTMGAAVAVAAYLRGAEVHAVSGPTGNPDTPWMPAAVTRHNVVSAKDMFAAASDLWPGMDAGVFTAAVADFSPIPIGPEKFKKDGAKDGFSVTYTPNPDILQTLAASKKPHQRVVAFAAETSNVRESALQKLERKNADMIVANTVNVADSGFGGGPNTC",
    "response#3": "MRILLGITGGIAAYKAANLIRGFSELGHEVTAVPTENALRFIGKPTLEALSGHPVETDMYVDVDMVRHVELGQQADLIVIAPATASFIARLAAGIADDLLLNAVLASKAPVVLAPAMHTEMWQNSATRANIETLAQRGIRVLEPSSGRLTGADSGPGRLPETNEIISE"
  },
  {
    "reference": "MHIMAVTVSDICENTKLAREMSLMGNYETASVYYQGCIQQIHRLLATIEDPLRKRKWQTIQGQIAQEYDVIKNITQTLALFKSGESNLDNPRSGRSVFQAFDEEPTRDPDVWPPASTTPRAPQPMDHHHHHKPVRGPGSVKRSDTRNTRAKTTTAARKTRKGSAGTASHKKDDPSTQSKRRNVPPGSAKSQKDNNNEEESEAAPDEKKFDVSGIDHDLVEILERDIVQKDPNTHWDDIADLQEAKRLLEEAVVLPMWMPDFFKGIRRPWRGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRILFEMARFYAPSTIFIDEIDSLCSRRGSESEHEASRRVKSELLVQMDGMSSITDEPGKVVMVLAATNFPWDIDEALRRRLEKRIYIPLPTEEGREVLLKINLKEVKLSDDVILKNIAKKLGGYSGADITNVCRDASMMSMRRKIAGLRPSEIRNLDKEELDLPVTMQDFHDAITKCNKSVSQEDLDKYEKWMEEFGSS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that contributes to cell division, microtubule severing by functioning in microtubule with isomerase activity, ATP binding, ATP hydrolysis activity.2. The ATP binding site should be stable and able to withstand changes in environmental conditions.3. The designed protein should contain one or more AAA+ ATPase domains that are essential for its function.4. For general function, the protein need meet that Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. The designed protein sequence is ",
    "response#1": "DIADLHEAKRLLEEAVVLPMWMPDFFKGIRRPWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPSTIFIDEIDSLCSRRGSESEHEASRRVKSELLVQMDGISSNSEDPSKVVMVLAATNFPWDIDEALRRRLEKRIYV",
    "response#2": "PWKGILLFGPPGTGKTMLAKAVATECKTTFFNVAAANLTSKYHGEGEKLVHLLFELAKCYAPSTVFIDEIDSLCSTRGQSNEHEASRRAKSELLIQMDGVSGALGNDDLSKMIMVLGATNHPWDLDDAMRRRLEKRIYIPLPTVAEREGL",
    "response#3": "PWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPSTIFIDEIDSLCSRRGSESEHEASRRVKSELLVQMDGISSNSEDPSKVVMVLAATNFPWDIDEALRRRLEKRIYIPLPELNEREQIFRIHLKKD"
  },
  {
    "reference": "MQVTFLGTSSGVPTRARNVSAVALRLPQRSEMWLFDCGEGTQHQFLRSELRLSQLRRVFVTHMHGDHVFGLPGLLASLGLAGNSNEGVDLYGPDPLESYLNGVLRTSSTRIGYPLKVHRVHDCAENGTLVYEDDDLMVRCTPLTHRVPAYAYRVDQKPLAGRFDIAKAKDLQIPPGPIYAQLKRGDTVTLEDGRVIDGTTLCGPERPGVSVVYCTDTVFCEAAVDLAQGADLLIHESTFAHQESEMAYQKQHSTSTMAAQTAAEANVGQLVLTHLSPRYAPGNAVTPEDLLKEAQAIFPNTCLAKDFLTLEVKPRCNSL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity.2. The protein must have high specificity towards 3'-tRNA processing endoribonuclease activity, zinc ion binding.3. For general function, the protein need meet that Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity; Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.4. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding.5. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in Endonucleolytic cleavage of RNA, removing extra 3' nucleotides   from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy   group is left at the tRNA terminus and a 5'-phosphoryl group is left   at the trailer molecule. are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "MTNVSAIALQLLEERGGTWLFDCGEATQHQILHTSVRPRRIEKIFITHLHGDHIFGLPGLLGSRSFQGGTTPLTVYGPKGIEQFIEVALSVSTTHVKYPLEIVEITEEGIVFEDKQFYVETKRLSHGIECFGYRIVEKDIQGPLLVEKLREANVKPGPIFKRLKDGEIVELEDGRVLDGKDFIGPPQKGRIITILGDTRYCDASRILAQDADVLVHEATFAAADQTQAHDYFHSTTEQAARIALEANVKRLILTHVSLKYMQEKGLEYEIVSLAEEIRIS",
    "response#2": "NVPAIALKYGGEIILWDIGEGTLRQLDTTKLSPMKIEKIFITHFHGDHYLGLMSLIQTMTLWKRERPLYIYGPKYTFEFIQNYLKSGFFRPSFEIHIHELGETRLKFKDYEIWSFKVDHGIPALGYVFKENDKRGNFDVEKIRELGLKPGPWMKKLEIKGKIEINGKLIYLEDVTGEPKKGVKIVYTGSAADYAKLCGNVSAIALQLLEERGGTWLFDCGEATQHQILHTSVRPRRIEKIFITHLHGDHIFGLPGLLGSRSFQGGTTPLTVYGPKGIEQFIEVALSVSTTHVKYPLEIVEITEEGIVFEDKQFYVETKRLSHGIECFGYRIVEKDIQGPLLVEKLREANVKPGPIFKRLKDGEIVELEDGRVLDGKDFIGPPQKGRIITILGDTRYCDASRILAQDADVLVHEATFAAADQTQAHDYFHSTTEQAARIALEANVKRLILTHISSRYNNRPEYAKELYPEEL",
    "response#3": "MDYNVSAIALQLLEERGGTWLFDCGEATQHQILHTSVRPRRIEKIFITHLHGDHIFGLPGLLGSRSFQGGTTPLTVYGPKGIEQFIEVALSVSTTHVKYPLEIVEITEEGIVFEDKQFYVETKRLSHGIECFGYRIVEKDIQGPLLVEKLREANVKPGPIFKRLKDGEIVELEDGRVLDGKDFIGPPQKGRIITILGDTRYCDASRILAQDADVLVHEATFAAADQTQAHDYFHSTTEQAARIALEANVKRLILTHISSSGKNGIKRSE"
  },
  {
    "reference": "MDNLSPEEVQLRAHQVTDESLESTRRILGLAIESQDAGIKTITMLDEQGEQLNRIEEGMDQINKDMREAEKTLTELNKCCGLCICPCNRTKNFESGKNYKATWGDGGDNSPSNVVSKQPSRITNGQPQQTTGAASGGYIKRITNDAREDEMEENLTQVGSILGNLKNMALDMGNEIDAQNQQIQKITEKADTNKNRIDIANTRAKKLIDS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.2. A protein that contributes to protein transport, regulation of exocytosis by functioning in GABA-ergic synapse, glutamatergic synapse, postsynapse with SNAP receptor activity. The designed protein sequence is ",
    "response#1": "VFRPGSIEQAVEEIRVVVRPVEDGEIQGVWLLTEVDHWNNEKERLVLVTEQSLLICKYDFISLQCQQVVRIALNAVDTISYGEFQFPPKSLNKREGFGIRIQWDKQSRPSFINRWNPWSTNVPYATFTEHPMAGADEKTASLCQLESFKALLIQAVKKAQKESPLPGQANGVLC",
    "response#2": "VFRPGSIEQAVEEIRVVVRPVEDGEIQGVWLLTEVDHWNNEKERLVLVTEQSLLICKYDFISLQCQQVVRIALNAVDTISYGEFQFPPKSLNKREGFGIRIQWDKQSRPSFINRWNPWSTNVPYATFTEHPMAGADEKTASLCQLESFKALLIQAVKKAQKESPLPGQANGVLG",
    "response#3": "VFRPGSIEQAVEEIRVVVRPVEDGEIQGVWLLTEVDHWNNEKERLVLVTEQSLLICKYDFISLQCQQVVRIALNAVDTISYGEFQFPPKSLNKREGFGIRIQWDKQSRPSFINRWNPWSTNVPYATFTEHPMAGADEKTASLCQLESFKALLIQAVKKAQKESPLPGQANGVLC"
  },
  {
    "reference": "MAELLIATRNKGKLKELQHMLNPLELAIRSIADFPDLPEIAETGNTFEENAVLKAQTIADLTGMVTMADDSGLEVDALGGKPGVYSARFAGENATNSQNNELLLNMMSDVPAQLRGARFVSVIAVARPGKLLGTVRGFCEGIITDRMRGNGGFGYDPLFFLKDYNMTFAELSLELKNKVSHRAKALNKAVILLEKIILENAACFDPKDLRKRQTELKEDT",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be optimized for ribonucleoside triphosphate phosphatase activity activity in order to improve purine nucleoside triphosphate catabolic process.2. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions. The designed protein sequence is ",
    "response#1": "SEYIEGTSLNLISNEKYPFEDVAMLLKKIHNVEWKSDNDYNPFKRLEKYEQYCIEKGFVLPKSYLNIRDKFFTYKDYLQSLPKSFAHGDSQPSNFIITDQGLKVVDFEFTGNIDHIYDIACFANMRISDGEQLLKARS",
    "response#2": "SEYIEGTSLNLISNEKYPFEDVAMLLKKIHNVEWKSDNDYNPFKRLEKYEQYCIEKGFVLPKSYLNIRDKFFTYKDYLQSLPKSFAHGDSQPSNFIITDQGLKVVDFEFTGNIDHIYDIACFANMRISDGEQLLKALG",
    "response#3": "SEYIEGTSLNLISNEKYPFEDVAMLLKKIHNVEWKSDNDYNPFKRLEKYEQYCIEKGFVLPKSYLNIRDKFFTYKDYLQSLPKSFAHGDSQPSNFIITDQGLKVVDFEFTGNIDHIYDIACFANMRISDGEQLLKAR"
  },
  {
    "reference": "MAGIKVFGHPASTATRRVLIALHEKNVDFEFVHVELKDGEHKKEPFILRNPFGKVPAFEDGDFKIFESRAITQYIAHEFSDKGNNLLSTGKDMAIIAMGIEIESHEFDPVGSKLVWEQVLKPLYGMTTDKTVVEEEEAKLAKVLDVYEHRLGESKYLASDHFTLVDLHTIPVIQYLLGTPTKKLFDERPHVSAWVADITSRPSAQKVL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The ideal protein would be one that plays a critical role in toxin catabolic process, response to water deprivation, response to cadmium ion, response to oxidative stress, defense response through quercitrin binding, camalexin binding, cobalt ion binding, copper ion binding, glutathione binding within mitochondrion, plant-type vacuole, plasmodesma, cytoplasm, plant-type cell wall.2. The designed protein should have a high specificity for glutathione binding, with minimal off-target effects.3. The protein contains novel GST N-terminal domains that confer a unique function or activity.4. For general function, the protein need meet that May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides.; Involved in camalexin biosynthesis by probably catalyzing the conjugation of GSH with indole-3-acetonitrile (IAN)5. We are looking for a protein with broad substrate specificity for glutathione + RX = a halide anion + an S-substituted   glutathione + H(+). The designed protein sequence is ",
    "response#1": "MTFATVYIKPHTPRGDWLASLGQYVGLEIKTVDYKSAEASKFEELFPLKRVPALVTPNGFQLTELIAIVEYIVAKGSKPDAVVLLIAQEKERNIFDQRAIENELLDRKIHVIRRRFEDVSERGSLDQNRRLFMEDQEVAVVYFRDGYMPSQYNAQNWEARLLLERSCAAKCPDIATQLLAXYPSSLRTKIDELNDYFYDTVNNGVYKTGFATTAEAYEKNVRVVFQGLDRLEQVLKESKGPFLLGDHLTETDVRLYTTIVRFDPVYVQHFKCNIGTIRHNYPHINQWLKRLYWKHPAFHETTDFKHIKCHYTQSHTQINPLGITPLGE",
    "response#2": "MTFATVYIKPHTPRGDWLASLGQYVGLEIKTVDYKSAEASKFEELFPLKRVPALVTPNGFQLTELIAIVEYIVAKGSKPEKCPITRKDPPSSLRTKIDELNDYFYDTVNNGVYKTGFATTAEAYEKNVRVVFQGLDRLEQVLKESKGPFLLGDHLTETDVRLYTTIVRFDPVYVQHFKCNIGTIRHNYPHINQWLKRLYWKHPAFHETTDFKHIKCHYTQSHTQINPLGITPLGG",
    "response#3": "MTFATVYIKPHTPRGDWLASLGQYVGLEIKTVDYKSAEASKFEELFPLKRVPALVTPNGFQLTELIAIVEYIVAKGSKPLDGKWVADSDVIVGLLEEKYPEPSLKTPPEFASVGSKIFGAFVTFLKSKDANDGSEKALVDELEALENHLKTHSGPFVAGEKITAVDLSLAPKLYHLEVALGHYKNWSVPESLTSVRNYAKALFSRESFENTKAKKEIVVAP"
  },
  {
    "reference": "MSQPRPTTLNFITGNKNKLAEVQAILGSVDGLTLQSRNVEGAEIQGTIEEVARDKCSRAAEVVGGPVLTEDTALEFQALKGLPGPYIKYFLTALGHEGLNNMLAAYEDKTAFAVCTFGYCAGPGQEPILFQGRTKGKVVPARGPPNFGWDPVFEYEGKTYAEMDKAEKNLISHRGKALDKLKVWLAGGESEP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions..2. The designed protein should have a binding site that is specific to the target Mg(2+) ligand.3. The protein should have ITP diphosphatase activity, nucleotide binding, dITP diphosphatase activity and be active in nucleus, cytoplasm. The designed protein sequence is ",
    "response#1": "LCTVVYL",
    "response#2": "SKMPS",
    "response#3": "MK"
  },
  {
    "reference": "MFYWLIELTNTFPSLSVFRGVLNVFRYITFRTGGAVVTGALFVFLFGPWIIDHLRLRQGKGQPIRTDGPQSHIISKKGTPTMGGLMILSGLVVSTVLWANPLNPYVWIVLAVTLGFGFVGFYDDYLKVTKQSHSGFAGRARLLIEAAIALVACYALVRLGRDPSSTGLAIPFFKDLVIKFGWMYVIFGAFVIVGAGNAVNLTDGLDGLAIVPVMIASASFGLIAYLAGNAVFSDYLQIHYVAGTGELAVLCGAVLGAGLGFLWFNAPPASIFMGDTGSLALGGMLGSIAVAVKHEIVLAVIGGLFVLEAVSVIVQVASFKLTGKRIFRMAPIHHHFEQLGWTEPQIVIRFWIISVMLALVGLSTLKLR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein have enhanced di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-alpha-   D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-   alanyl-D-alanine = di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-   alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-   alanyl-D-alanine + UMP.2. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.3. For general function, the protein need meet that Catalyzes the initial step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan: transfers peptidoglycan precursor phospho-MurNAc-pentapeptide from UDP-MurNAc- pentapeptide onto the lipid carrier undecaprenyl phosphate, yielding undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide, known as lipid I.4. The designed protein should have at least Helical transmembrane domains to ensure proper localization.5. The protein design should consider the endogenous proteins and enzymes present in the Cell wall biogenesis, peptidoglycan biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MIKLTELAADCKAGTPTMGGALIDSRCVAAGDGFIFCAMPGGARDGRRYAEEAVARGAAAILCERDVELDFGVRFVAVDDTRAALAMVAAAYYGHPTDALKVIANATNLTDGLDGTEVLIIGGSQGARVLNQTMPAVAARLAGILTLWHQVGKGALEEVNRAYAANGETQHKVVEFIDDMAAAYAWADAVVCRAGALTVSEIAAAGLPALFVPFMHKDRQQYWNARLLEQACAAKIIEQPAFSVERVSDVLAGWDRPTLLTMAQRARAAAIPDATEAALALARSLKAAAGQHIVLIIGGSQGARVLNQTMPAVAARLAGILTLWHQVGKGALEEVNRAYAANGETQHKVVEFIDDMAAAYAWADAVVCRAGALTVSEIAAAGLPALFVPFMHKDRQQYWNARLLEQACAAKIIEQPAFSVERVSDVLAGWDRPTLLTMAQRARAAAIPDATEAALALARSLKAAAGHTA",
    "response#2": "MKLGELLNGLQELWPITEGEPDMQVIGVXTDSRKVRPGFVFAALPGXRTDGHXFLXDVVSRGAAAXMXQAXGXAQXLSXGXGXSXXXAXEXXSXXPRXAXGXPAXVVLIIGGSQGARVLNQTMPAVAARLAGILTLWHQVGKGALEEVNRAYAANGETQHKVVEFIDDMAAAYAWADAVVCRAGALTVSEIAAAGLPALFVPFMHKDRQQYWNARLLEQACAAKIIEQPAFSVERVSDVLAGWDRPTLLTMAQRARAAAIPDATEXVRXGXPXGXKXAVLIIGGSQGARVLNQTMPAVAARLAGILTLWHQVGKGALEEVNRAYAANGETQHKVVEFIDDMAAAYAWADAVVCRAGALTVSEIAAAGLPALFVPFMHKDRQQYWNARLLEQACAAKIIEQPAFSVERVSDVLAGWDRPTLLTMAQRARAAAIPDATEEXRRXGXAYXGEXALXQTXEXXVXAXQRXSXHXRXPXGGXTXVXPXQVXGXIMCMMAXPXSXTA",
    "response#3": "MKLSELLQDVELVSIQGNLDVEVTDMTCDSRRAGPGSLFVAVRGAETDGRRFIPEVAAKGAACVVADRECEVDVPQLVVRDVRKSMGMAAAALHGHPSRKLALIA"
  },
  {
    "reference": "MGGKWSKAKGWPAVRERMRRARPAEPAAEGVGAVSRDLDKHGAITSSNTAATNADSAWLEAQEDEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPEEVEKATEGENNCLLHPISQHGMDDPEKEVLMWKFDSRLAFHHVAREKHPEYYKDC",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein with GTP binding functions is required to enable suppression by virus of host autophagy, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II.2. For general function, the protein need meet that Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis3. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.4. The protein design should include a Dileucine internalization motif; necessary for CD4 internalization, Diacidic; necessary for CD4 internalization motif to ensure proper function. The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPEIRERMRRTPPAAEGVGAVSQDLDKHGAITSSNINHPSCVWLEAQEDEEVGFPVRPQVPLRPMTFKGAFDLSFFLKEKGGLDGLIYSKKRQEILDLWVYNTQGFFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPREVEEANKGENNSLLHPICQHGMEDEDKEVLMWKFDSSLARRHIARELHPEFYKDC",
    "response#2": "MGGKWSKSSLVGWPAIRERMRRAEPAADGVGAVSRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVDPREVEKETEGENNCLLHPISQHGMDDPEREVLVWRFDSSLAFKHVARELHPEYYKDC",
    "response#3": "MGGKWSKRKVEWPAVRERMRRAEPAAEGVGAASQDLDKYGALTSSNTPATNAACAWLEAQQEEEEVGFPVKPQVPLRPMTYKGAVDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVDPEKVEEANAGENNSLLHPMSLHGMEDPEKEVLMWKFDSRLAFHHMARELHPEYYKDC"
  },
  {
    "reference": "MKAIKLTGHERPLTQVKYNKEGDLLFSCSKDSSASVWYSLNGERLGTLDGHTGTIWSIDVDCFTKYCVTGSADYSIKLWDVSNGQCVATWKSPVPVKRVEFSPCGNYFLAILDNVMKNPGSINIYEIERDSATHELTKVSEEPIHKIITHEGLDAATVAGWSTKGKYIIAGHKDGKISKYDVSNNYEYVDSIDLHEKSISDMQFSPDLTYFITSSRDTNSFLVDVSTLQVLKKYETDCPLNTAVITPLKEFIILGGGQEAKDVTTTSANEGKFEARFYHKIFEEEIGRVQGHFGPLNTVAISPQGTSYASGGEDGFIRLHHFEKSYFDFKYDVEKAAEAKEHMQEAN",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.. The designed protein sequence is ",
    "response#1": "FFNHGKGRDLIIDMFLYKPQYLNAIQTMCPHILRYLATAVIINRGRRNALKDLIKVIQQESYTYRDPITEFLEHLYVNFDFEGARKKLHECQTVIVNDFFIIGCLTEFVENARLMIFETFCRIHQCITIGMLADKLNMKPDEAECWIVNLIRNARLDAKIDSKLGHVVMGTQPC",
    "response#2": "MQLIEKALDSRRREATLESGLEQLETSTAQMIEWLERMLHYVEDVNKNGEKPGDAQIGRQLMDIVTASSNNMQPEKLDTLVKNTLRDYVMVSYLAKLTQTQLQVHERV",
    "response#3": "MQLIEKALDSRRREATLESGLEQLETSTAQMIEWLERMLHYVEDVNKNGEKPGDAQIGRQLMDIVTASSNNMQPEKLDTLVKNTLRDYVMVSYLAKLTQTQLQVHERE"
  },
  {
    "reference": "MTELAPKASPAHSIARVLVIDDSAVARGLMTRWVEEDSDLTLIGSAVDGEQGLRKAEELKPDLIVLDVEMPKMDGLAALPLLLKAVPGCKIVMASTLTRRGGEVTIRALSMGAADYASKPQAGRLAGAEEFRRDLLLKLKALAPRPIPPVPTQRDMAPSPAPATPAAPGAPVARSITPPPPPSSSAPAKKAFAPVAQPPQGRGTPRNTARPEIIAIGSSTGGPQALRDVIAAFPADVRSPIVIAQHMPALFTKILAEHLTKAGKLVCKEAEDNERLKPGCVYIAPGDFHMTIRKDAAGFYAVLDQTPPINFCRPAVDPLFQSVAEVTRGAALGIVLTGMGHDGREGARMLRSVGGTILAQDEATSVVWGMPGAVAEAGLADEILSLADMGPGIVRRAKGGN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The protein must be able to protein-glutamine glutaminase activity.3. Prioritize Pro residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "VRIGLVGKYVELPDAYKSIIEGFVHAGSKNECKVKVEYIHSEGIYPDNAHEKLGHLDGVLVAPGFGSRGIEGKIDTIRYVRENNVPFFGICLGMQCAVIEYGRNVIGLEGAHTTEIDENCAHPVINMMEEQKKITEKGGTMRLGSYPCDLKKGSKAFAIYGRTHITERHRHRYEFNSAYLQQYEDAGMIASGINPESKLVEIIELKNHPFFIGGQFHPELKSTVANPHPLFVKFIQENT",
    "response#2": "VTIHVVGKYVGYEDSYKSLNEALYHGGFKHRLRVNIRWVEAEALEQPGGEQLLDGANGILVPGGFGDRGTRGMMRAAQIARERNIPYLGICYGFQWAAVEYARHVCGLTEADSTECAPDTPQKVIYKLRDLLGIDDLGGTMRLGSYPCELKAGSRAYQLYGEPVIAERHRHRYEFNCLYEKALTERGLEIVGRSHDGKFVEIVELPSHPWYVAVQFHPEFKSKPLKPHPLFAGFR",
    "response#3": "LPDAYKSIIEGFVHAGSKNECKVKVEYIHSEGIYPDNAHEKLGHLDGVLVAPGFGSRGIEGKIDTIRYVRENNVPFFGICLGMQCAVIEYGRNVIGLEGAHTTEIDENCAHPVINMMEEQKKITEKGGTMRLGSYPCDLKKGSKAFAIYGRTHITERHRHRYEFNSAYLQQYEDAGMIASGINPESKLVEIIELKNHPFFIGGQFHPELKSTVANPHPLFVKFVY"
  },
  {
    "reference": "MSGSSFGSIFKIATWGESHGKGIGVVVDGCPAGLTLNEEMIQTFLNRRKPGQTKYSTPRKEDDLVTILSGVFEGKTTGTPISMMIANETARSADYSEIASFYRPGHADYTFDAKYGFRDYRGGGRSSGRETIGRVAAGAIAAALLKELGIEVFTYTKSIGPIQIDYHKCQKENLTLSPLCMPDLEASQKAEDYLEQCIHNLDSSGGMIECIISGVPAGIGEPVFDKLDAQLAKAIFSIGAVKGFEIGSGFEVAKQLGSENNDGFAFDANGKLIKLTNHSGGILGGISDGSEIIFRAAIKPTPSIKKEQQTVNKSGENINVSIKGRHDPIIVPRAVVVVEAMAALTLADLLLSGMSSKMDYVKKIYQK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Ideal protein should have a binding affinity for key enzymes in the chorismate biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "EPECHDNAMQLVQAMRHFSTFVYGLNLCKEEADIETLLKFSSPIYRLELAMVGRLFAQSPELYADIIFAQQESQHAIGDYLDNYREALELLKRGDREEFINQFL",
    "response#2": "EPECHDNAMQLVQAMRHFSTFVYGLNLCKEEADIETLLKFSSPIYRLELAMVGRLFAQSPELYADIIFAQQESQHAIGDYLDNYREALELLKRGDREEFINQFL",
    "response#3": "EPECHDNAMQLVQAMRHFSTFVYGLNLCKEEADIETLLKFSSPIYRLELAMVGRLFAQSPELYADIIFAQQESQHAIGDYLDNYREALELLKRGDREEFINQFL"
  },
  {
    "reference": "MKITRMHGAGGKVMQELIKDVILKNLEITSVNGGIGLESLDDSATIPIGDKEIVFTVDGHTVKPIFFPGGDIGRLAVSGTVNDLAVMGAKPLALSLSLIIPEGFNLEDLEKIVKSINETSKEAEVAIITGDTKVSDGVDDIIISTAGIGIVDRGKAIRDCNVQEGDAIIVSGNIGEHGLAILLSREGFDFETNIKSDVAPINKLIERVLEEGIQINAMKDPTRGGLADALNEMAEKSNIGITIFEDKIPISDEVQSICDILGLDPLTIANEGKVVMAVKKEDAERCLEILREHPLGKNAEIIGYATKEHKGVIIETIVGRRIVDMPIGDPIPRVC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design must be focused on enhancing the efficiency of protein maturation. The designed protein sequence is ",
    "response#1": "NSTYRSKKDEITLRKTFIEAGYKVRVIKDTNLIKTTNILSEFSEFAKKKKVCAVIVFILSHGVVNGEVYVGSDRCNLNYMVNAMDTEILRGVPKMLFVQINKEYRSVYENFKDVIVKFMSNTNLATLPDTTVVSIEDPCPAHAEYKPASPDVPLDFYYRESWYTNQSKDVTRVGSPMIQELCKLLRIDAEFCEIMALLDQ",
    "response#2": "NSTYRSKKDEITLRKTFIEAGYKVRVIKDTNLIKTTNILSEFSEFAKKKKVCAVIVFILSHGVVNGEVYVGSDRCNLNYMVNAMDTEILRGVPKMLFVQINKEYRSVYENFKDVIVKFMSNTNLATLPDTTVVSIEDPCPAHAEYKPASPDVPLDFYYRESWYTNQSKDVTRVGSPMIQELCKLLRIDAEFCEIMALLDC",
    "response#3": "NSTYRSKKDEITLRKTFIEAGYKVRVIKDTNLIKTTNILSEFSEFAKKKKVCAVIVFILSHGVVNGEVYVGSDRCNLNYMVNAMDTEILRGVPKMLFVQINKEYRSVYENFKDVIVKFMSNTNLATLPDTTVVSIEDPCPAHAEYKPASPDVPLDFYYRESWYTNQSKDVTRVGSPMIQELCKLLRIDAEFCEIMALLDEP"
  },
  {
    "reference": "MEADWDELSRIPVPPPSPHALPTVATTIAFDDVMELLWVGNEYGRITSFYGPELQRYTSVRAHPVAEGIVRQILFHERGVISLSSRSVHMITRRGLTQWHVAHEEMVDLRCMSFTAQLNRVIVAGCQKVMFTIDIDKGVIVDKLPTEYNYTMMKKSRYLCAATDTGSVNALSLSDFRVVKSWKAHGTAVNDMDARNDLLVTCGFSVRHLGSPIVDPLANVYDLKTLSPLPPIPFHAGAAYVRMHPKLSTTSFVASQTGQLQVVDLMNPNSINLRQANVSFMLGIDISPSGEALAINDAECMVHLWGSPAKIHFNEMSKEVELADVTHRPPPLDWSPDTPLNMIGMPYYHERLFSAWPSHLVFEVGSPPAPIDTGLIPYLRPAEIGHCAPNPKKTRRYQVENTRAVATTEPALIAPKFLSEKAREQSKKSDGSVAEAAGALAGAKLNGEAEDDPLLKYSNVEIKYSRFGVDDFDFRFYNQTNFSGLETHIANSFTNALLQLLKFIPLVRNVALHHAASSCVFENCLLCEMGYLFDMLEKANGQNCQATNLLKTFSSFREAASLGILEENLTNKSLSSAIQAVNRFFLGQIAHDFRTIMPNSDDLEQRLATIASESIQCRYCGNEIVRPGNSLVNELIYPNMDIKHTRRNPAFRFSNILRASIERETQNKGWCNYCRRYQPVGIRKSVHRMPLVMMLNAALNTPMARRLWAIPGWLPDEVGIVIDGGQTLCYEGEDLRMRVQANMPGLIVYELVGVVTEIDIPEHQKPHL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The a divalent metal cation binding site should be stable and able to withstand changes in environmental conditions.2. The designed protein must have at least one Exonuclease domain.3. The protein must exhibit the following characteristics: Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover; PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein pab1; PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex.4. The protein must be engineered to have a cooperative binding mechanism with a divalent metal cation to ensure proper regulation.5. The desired protein should be able to perform Exonucleolytic cleavage of poly(A) to 5'-AMP.6. The protein must exhibit poly(A)-specific ribonuclease activity. The designed protein sequence is ",
    "response#1": "MEGMFSELHSVVSEVGVPVTAVHFELQEEMLWMGNHRGHVTSFFGPTMGRYSSFQVHATDDIRHIQSLETGVLFLSKCSLKCYTRGGLVIFDYPMEEGSDMHSLLMTDNNTLLMGGLQNYVVEVDLNTVQETQKFTVEVPGVAIMRQSNRFFFCGHTSGKVTLRDLRTFKMEHEFDAFSGSLSDFDVHGNLLAACGFSSRGLNGLSCDRFLMVYDLRMMRAVTPLQVHVDPLFLRFIPTYTSRLAIISQTGQCQFCEPTGLANMADIFHVNTVGQLLMSFDVSSSKQALAFGDSGGCVHLWAGLEPHIPNAYCNCMIQVGKIGSFGSQAEGQRPNPALLSLVPQVLYFLEPIRCLVQNHLCQKEFCLACELGFLFHMLDLSRGDPCQASNFLRAFRTIPEASALGLILADSDEQTGKAKLGRLIQSWNRFILAQLHQETQEQEGPQAYRGASSSSLGSSGESVIGKLFGCEVENSSLCRCGKETVRSSLTLLFTMHYPEHNSPEKVREYDFAEILKRSICLEQS",
    "response#2": "AGLEPHIPNAYCNCMIQVLYFLEPIRCLVQNHLCQKEFCLACELGFLFHMLDLSRGDPCQASNFLRAFRTIPEASALGLILADSDEQTGKARLGRLIQSWNRFILTQLHQETQEQEEPQLKYTSALGSSGESAIGKLFGCEVENSSLCCCGKETVRSSLTLLFTMHYPEQNSQEKTIKEYDFAEILKKSICLEQSTQAWCDNCEKYQPTVQTRNIRCLPDVLVINCEVNSAKEAEFWKVQAEYAFNKKR",
    "response#3": "MEGMFSELHSVVSEVGVPVTATHFDLQEEMLWMGNHRGHVTSFFGPTMDRYSSFQVHAADDIRHIQSLETGVLFLSKSNLKCHTRGGLVMFEYPMDEGADMHSLLMTDNNCLLMGGLQNYVVEFDLNTVQEAQKFTVEVPGVAILRQTNRYFFCGHTSGKITLRDLRTFKMEQEFDAFSGSLSDFDVHGNLLAACGFSSRGLNGLSCDRFLMVYDLRMMRAVTPLQVHVDPLFLRFIPTYTSRLAIISQTGQCQFCEPTGLANMADIFHVNTVGQLLMSFDVSASKQALAFGDSGGCVHLWAGLEPHIPNAYCNCMIQVLYFLEPIRCLVQNHLCQKEFCLACELGFLFHMLDLSRGDPCQASNFLRAFRTIPEASALGLILADSDEQTGKARLGRLIQSWNRFILTQLHQETQEQEEPQLKYTSALGSSGESAIGKLFGCEVENSSLCCCGKETVRSSLTLLFTMHYPEQNSQEKTIKEYDFAEILKKSICLEQSTQAWCDNCEKYQPTVQTRNIRCLPDVLVINCEVNSAKEAEFWKVQAEYAFNKSGLENHITNSYANSLLQLLRFTPILRNLALQHAATSCLDQHCLLCELGYLCDSMEKARGASCQATNMLKMLSQHPAAANLHLLDGDRIASSSAMKIQGLLRFLLDFMSRDYGKTSPGINDLENATATSSQSLIRCDQCKSETSRPQTNYVQDLAYSPVTSNGNKGVDVADSYLAAQQGVARGRMAPAKLPSFSQILKKSIEREQVTRGWCTTCRGYQPLSMRKIIKSIPQVLAANTCISSVEEKKLWATPGWLPEEVGFIIDKEH"
  },
  {
    "reference": "MASHDSYTGRLPGAFMNPGTSSFTEFLASYNPDLLPGRHMTALAGGMPGNVEAPHATTIVAVTFPGGVVMAGDRRATAGNMIAQRDVEKVFRADEFSAVAIAGTAGIGMEIVRLFQVEIEHYEKMEGRTLSLEGKANRLATMIRANLGMAMQGLVAVPLFAGYDTEREVGRIFSYDPAGGRYEEHEHHSIGSGSVFARGALKKLWRPDLSAQDAALVCVQALYDAADDDSATGGPDLIRKIYPVVATVTADGFRRLPEEEVGELARIVVDGRHDSPGGPTAPLR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be able to modulate modification-dependent protein catabolic process in a way that leads to a desirable outcome.2. A protein that is capable of catalyzing reactions within the proteasomal Pup-dependent pathway pathway with high efficiency and specificity.3. The protein must exhibit the following characteristics: Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.. The designed protein sequence is ",
    "response#1": "VLLYGPPGCGKTLIAKAVANSLAKQVSKARGEDHKDAKSFFLNIKGPELLNKFVGETERHIRLIFQRAREKASEGTPVIVFFDEMDSIFRTRGSGVSSDVETTIVPQLLSEIDGVEGLENVIVIGASNREDMIDPAILRPGRLDVKIKIT",
    "response#2": "VLLYGPPGCGKTLIAKAVAASLAGMVDPHGTEGATSYFLNVKGPELLNKYVGETERHIRLIFARAREKASQGMPVVVFFDEMESLFRTRGTGLSSDVETTIVPQLLAEIDGVERLDNVIVIGASNREDMIDPAILRPGRLDVKIKIL",
    "response#3": "VLLYGPPGCGKTLIAKAVANSLAKKIAEARGEDAKEAKSFFLNIKGPELLNKFVGETERHIRIIFQRAREKASEGTPVIVFFDEMDSIFRTRGSGVSSDVETTVVPQLLSEIDGVEGLENVIVIGASNREDMIDPAILRPGRLDVKIKIL"
  },
  {
    "reference": "MGKHRGKKYLEVAKLVEIGKLYDIREALELVQKTKTAKFTETVEVALRLGVDPRHADQQIRGTVVLPHGTGKTVKILAITSGENIEKALAAGADYAGAEEYINQIQQGWLDFDLVIATPDMMPKIGRLGKILGTKGLMPNPKSGTVTPDIAAAVSEFKKGKLAFRVDKLGSIHAPIGKVDFDLDKIEENFKAFMDQIIRLKPASSKGQYLRTVAVSLTMGPGVKMDPAIVGKIVG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA..2. The ideal protein would be one that plays a critical role in regulation of translation, translation, maturation of LSU-rRNA through structural constituent of ribosome, RNA binding, rRNA binding, tRNA binding within cytosolic large ribosomal subunit. The designed protein sequence is ",
    "response#1": "SHFRS",
    "response#2": "MXR",
    "response#3": "MI"
  },
  {
    "reference": "MKVKGTQMSWLNLWIWGTLITGLVIMCNTSDNLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQELVLENVTENFNMWKNEMVNQMHEDVISLWDQFLKPCVKLTPLCVTLNCTDVTRNSSSPSNSTSNSTLENRQEMKNCSFNTTTVVRDKKQQVYALFYRVDIVPLEDNSSEYRLINCNTSAITQACPKVSFDPIPIHYCAPAGFALLKCNDKRFNGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNVKTIIVHLNKSVEIVCTRPGNNTRKSIRIGPGQTFYATGDIIGDIRQAHCNISEQKWNETLHNVRKRLEQYFPNKTIKFTSSSGGDLEITTHSFNCRGEFFYCNTSGLFNSTYTPTYNDTFNDTAIITIPCRIKQIINMWQEVGRAMYAPPIAGNITCRSNITGILLVRDGGVSNETNDTETFRPGGGDMRDNWRNELYKYKVVEVKPLGVAPTAAKRRVVEREKRAAIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLKAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKTQKEIWDNMTWMQWDKEISNYTYTIYSLLEESQSQQERNEKDLLALDSWKNLWTWFDISNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSLQTLIPNPVEPDRPERIEEGGGEQGRDRSVRLVSGFLTIAWDDLRSLCLFLYHRLRD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Transmembrane protein gp41: Acts as a class I viral fusion protein; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm..2. The protein should contain a Helical membrane-spanning region.3. Incorporating a Di-leucine internalization motif, YXXL motif; contains endocytosis signal motif is critical for the successful design of the protein.4. The protein should be designed to localize specifically to virion membrane in order to perform its role in mitigation of host antiviral defense response, positive regulation of plasma membrane raft polarization, actin filament reorganization, positive regulation of establishment of T cell polarity more effectively.5. The designed protein should contain one or more Human immunodeficiency virus 1 envelope glycoprotein Gp120, Retroviral envelope protein GP41-like domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MWVTVYYGVPVWREAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQELVLENVTENFNMWKNDMVDQMHEDVISLWDESLKPCVKLTPLCVTLNCSTILNCSTLNCSTTQSGNDTCNNNMCAEMKNCSFNTTTELRDKNRKEYALFYKLDIVPLERANNRTENNSSEEYRLINCNTSTTTQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGKGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENLTNNAKTIIVHLNESVEIVCTRPNNNTRKSIRIGPGQTFYATGAIIGDIRQAHCNISKSKWNSTLQRVGKKLAEHFPNRTIRFNSSSGGDLEITTHSFNCGGEFFYCNTSGLFNSTFNDTYAPNNTDTNETITIQCRIKQIINMWQQVGRAMYAPPIAGNITCNSSITGLLLVRDGGTKTDDNTTETFRPEGGNMRDNWRSELYKYKVVEIKPLGIAPTEAKRRVVQREKRAVLGAMFLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLKAIEAQQHILQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNTSWSNKSLDRIWDNMTWMEWEKEIDNYTQIIYTLIEESQTQQEKNEQELLELGKWDSLWSWFDITNWLWYIKIFIMIVGGLVGLRIIFTVLSIVNRVRQGYSPLSFQTLIPSPEEPDRPERIEEEGGEQGRDRSIRLVSGFLALAW",
    "response#2": "MRVREILRNCQQWWIWGILGFWMLMICNVVGNLWVTVYYGVPVWRDADTTLFCASDAKAHETEVHNVWATYACVPTDPNPQEIHLENVTENFNMWKNNMVEQMQEDIISLWDESLKPCVKLTPLCVTLNCTNANLTKANLTNANSTKVTNMTDVPNIGNMTEEVKNCSFNITTELRDKKQKVYALFYKLDIVQINNGGNNSDNNTYRLINCNTSVIKQACPKVSFDPIPIHYCTPAGFAILKCNDKNFSGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLADNAKTIIVHLNKSVEINCTRPSNNTRTSITIGPGQVFYRTGDITGDIRKAYCEVNKIKWNETLRQVARKLKEHFNKTIIFQPPSGGDLEITMHHFNCRGEFFYCNTSQLFNSTWIGNETKEECNKLKKECNSTIILPCKIKQIINMWQGVGQAMYAPPIRGRINCVSNITGILLTSDGGSRNYENETFRPGGGNIKDNWRSELYKYKVVEIEPLGVAPTKAKRRVVEREKRAVGIGAVLLGFLGAAGSTMGAAATTLAVQTQKVLKGIVQQQDNLLRAIQAQQHLLRLSVWGIRQLRARLQALETFMQSQQLLNMWGCRGKPICYTSVQWNRSWGGDESIWNNLTWQQWDQQINNISYIIYGQIQEAQEQQERNEKSLLELDEWASIWNWLDITKWLWYIKIAIIIVGALIGLRVVMIILNLVKNIRQGYRPLSLQT",
    "response#3": "MWVTVYYGVPVWREAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQELVLENVTENFNMWKNDMVDQMHEDVISLWDESLKPCVKLTPLCVTLNCSTILNCSTLNCSTTQSGNDTCNNNMCAEMKNCSFNTTTELRDKNRKEYALFYKLDIVPLERANNRTENNSSEEYRLINCNTSTTTQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGKGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENLTNNAKTIIVHLNESVEIVCTRPNNNTRKSIRIGPGQTFYATGAIIGDIRQAHCNISKSKWNSTLQRVGKKLAEHFPNRTIRFNSSSGGDLEITTHSFNCGGEFFYCNTSGLFNSTFNDTYAPNNTDTNETITIQCRIKQIINMWQQVGRAMYAPPIAGNITCNSSITGLLLVRDGGTKTDDNTTETFRPEGGNMRDNWRSELYKYKVVEIKPLGIAPTEAKRRVVQREKRLLPLWRWGMIIFWIMMICNAKNLWVTIYYGVPVWKDAKTTLFCASDAKAYEKEVHNVWATHACVPTDPDPQELVLGNVTENFNMWKNDMVDQMNEDIISLWDQSLKPCVKLTPLCVTLNCSNDVTKNVTATSTKNCSFNVTTEITDKKKQERALFYRLDIVPIENSTEYRLINCNTSTIAQACPKISFDPIPIHYCAPAGYAILKCNNKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIIRSENLTNNAKTIIAHLNESIEINCTRPNNNTRKSIRIGPGQTFYATGDIIGDIRQAHCNISAERWNTTLQRVRKKLKEHFNKTITFEPSSGGDLEITTHSFNCGGEFFYCNTSQLFNSSHWFNTTTNQTELITLQCRIKQIINMWQGVGRAMYAPPIKGNITCRSNITGLLLTRDGGKNETETFRPGGGNMKDNWRSELYKYKVVEINPLGIAPTKAKRRVVEREK"
  },
  {
    "reference": "MISKLVMATNNANKLREAREILSPLGIEVLSQREAGADCEPDESGETFEENALIKAQTIYDLVELPVIADDSGLCVDAMDGRPGVHSARYAPKGKECAKLLEEMKDVPDEKRTAAFVCTVCYMDDSGYAFMTGTCKGSISHCEAGENGFGYDPVFLVGDRTMAELSAEEKNKISHRGAALRELYRYLKERYGE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.2. The designed protein must be able to perform dITP diphosphatase activity, nucleotide binding, XTP diphosphatase activity under specific conditions. The designed protein sequence is ",
    "response#1": "MPIGVPKVSFRNPGDEVSA",
    "response#2": "MISNSIKLN",
    "response#3": "MENRKT"
  },
  {
    "reference": "MKRIVSGDQVKVISGNEAGKVGVVRKVLYSSVGRRKEVYVIVSDVNVRRFVKKTQAGKKFDAKSYPIAVSNVALLAGDGFVSKVGFKLKDGTKKRIFKRTAEFV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be able to regulate translation.2. The protein must exhibit the following characteristics: One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit.. The designed protein sequence is ",
    "response#1": "MMGDEVVVITGKHKGKKGKTM",
    "response#2": "MPIGVPKVPFRSPGENPCTWLDLYRE",
    "response#3": "MSPVRVGDEVVIMRGDFKGHRGKVVRVDLN"
  },
  {
    "reference": "MKTKILLLNFIIIFFLINVNLAIKKDSPFKEIQKNNNNKKENHDAINYYEDEDGFDVTIKDCISILVPYGKCTKIKSPFKKCPYKSIFISSNSTTPIIPNNNYNNNNNNKNKNNGSNQYIINLFENSKCYEKVNRNKNKIFKNFHEEKNYKESKKNELIINCKDKKLNKFNNNEIRVVCPISSKFDSSTSSISINTLAILSLLFLIFINKLIN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Extracellular, Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.2. The protein must contain a signal peptide for proper functionality.3. The designed protein localizes to the membrane.4. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "MPGTIGGLALRVGQFLFAAAAIVIMVTGDEFTNYTAFCYLVAAMSLQFLWSFMLAILDTYALLIKRGLRNSVLLSLFVVGDWVTATLSLAAACSTAGVTVLFDNDLNYCGQMHCHRYQLSAAMAFLSWLI",
    "response#2": "MPGTIGGLALRVGQFLFAAAAIVIMVTGDEFTNYTAFCYLVAAMSLQFLWSFMLAILDTYALLIKRGLRNSVLLSLFVVGDWVTATLSLAAACSTAGVTVLFDNDLNYCGQMHCHRYQLSAAMAFLSWLL",
    "response#3": "MPGTIGGLALRVGQFLFAAAAIVIMVTGDEFTNYTAFCYLVAAMSLQFLWSFMLAILDTYALLIKRGLRNSVLLSLFVVGDWVTATLSLAAACSTAGVTVLFDNDLNYCGQMHCHRYQLSAAMAFLSWLL"
  },
  {
    "reference": "MSLFNHLLSRALPLWLLFFINFLVLLGKSQQEVLQVQVGIVLDTNATLAALSLRAINMSLSEFYNTHNGFKTRIVLNIRDSKRTVVGAAASALYLIKKREVVAIIGPGNSMQAPFLINLGNQSQVPIISFSASSPVLDSLRSPYFIRATHDDSSQVHAISAIIESFRWREVVPIYADNEFGEGILPYLVDAFQEINVRIRYRSAISVHSTDDLVKKELYKLMTMPTRVFIVHMLPDLGSRLFSIAKEIGMMTKGYVWIVTNGIADQMSVMGESSLENMHGVLGVKTYFSRSKELMYLETRWRKRFGGEELNNFECWGYDTATALAMSIEEISSNVNMSFSQTKRNTSRDDTGTDLDDLSFALSGPKLLQALATVSFKGVAGRFQLKNGKLEATTFKIVNIEESGERTVGFWKSKVGLVKSLRVNQTGIKISHSSHRLRPIIWPGDTIFVPKGWEFPTNAKKLRIAVPKKDGFNNFVEVTKDANTNAPTITGFCIDVFDTAMRQMPYAVPYEYIPFETPDGKPRGSYDEMVYHVFLGEFDGAVGDTTILANRSTYVDFALPYSETGIVVVVPVKDEREKGKWVFLKPLTRELWFLTAASFLYIGIMVWIFEYQASGDFRKQSIINKISNVFYFSFSTLFFAHMRPSESIFTRVLVVVWCFVLLILTQSYTATLTSMLTVQELRPTVRHMDDLRNSGVNIGYQTGSFTFERLKQMGYKESRLKTYDTPQEMHELFLKKSSNGGIDAAFDEVAYVKLFMAKYCSKYTIIEP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that May be involved in light-signal transduction and calcium homeostasis via the regulation of calcium influx into cells.; Glutamate-gated receptor that probably acts as non-selective cation channel2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The Cytoplasmic, Extracellular topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.4. The protein should exhibit Polar residues compositional bias.5. The designed protein should be involved in cellular response to amino acid stimulus, calcium-mediated signaling, calcium ion transport by localizing to plasma membrane and exhibiting signaling receptor activity, ligand-gated ion channel activity, calcium channel activity, glutamate receptor activity. The designed protein sequence is ",
    "response#1": "MLCINMSLADFYSSRPQFQTRLVVNVGDSKNDVVGAATAAIDLIKNKQVKAILGPWTSMQAHFLIEIGQKSRVPVVSYSATSPSLTSLRSPYFFRATYEDSSQVHAIKAIIKLFGWREVVPVYIDNTFGEGIMPRLTDSLQDINVRIPYRSVIPLNATDQDISVELLKMMNMPTRVFIVHMSSSLASTVFIKAKELGLMKPGYVWILTNGVMDGLRSINETGIEAMEGVLGIKTYIPKSKDLETFRSRWKRRFPQMELNVYGLWAYDATTALAMAIEDAGINNMTFSNVDTGKNVSELDGLGLSQFGPKLLQTVSTVQFKGLAGDFHFVSGQLQPSVFEIVNMIEKLRVLVPAGNITPQILEVKTDFKTGVTAATGYCIDVFETSILPFNYEVEYIPWPGAINYKNYNDLVYTLYSQKDKYDAAVGDITITDNRSLYVDFTLPFTDMGLAVVTAKDKSMWIIFKPLTLSLWLTIASFFILTGAIVWLIERHDNADFQGSCFQQIGTLLCFGFSTLVFAHRERLQHNMSRFVVIVWIFAVLILTSNYTATLTSVMTVQQIRGLKSNENIGFFSASIAANVVNDNPTFQGPRYKGLKTADDFTNALRNGTISFIVDEVPYVKLFVAKHPSEFVIVETESVTNGFGFAFQKGSPLVQKVSREIEKLRRTLG",
    "response#2": "MLRVGIPVKKGFLEFVDAKIDPISNAMTPTGYCIEIFEAVLKKLPYSVIPKYIAFLSPDENYDEMVYQVYTGAYDAVVGDVTIVANRSLYVDFTLPYTESGVSMMVPLKDNKNTWVFLRPWSLDLWVTTACFFVFIGFIVWILEHRVNTDFRGPPHHQIGTSFWFAFSTMNFAHREKVVSNLARFVVLVWCFVVLVLIQSYTANLTSFFTVKLLQPTVTNWKDLIKFNKNIGYQRGTFVRELLKSQGFDESQLKPFGSAVECDELFSNGTITASFDEVAYIKVILSQNSSKYTMVEPSFKTAGFGFVG",
    "response#3": "MLRVGIPVKKGFLEFVDAKIDPISNAMTPTGYCIEIFEAVLKKLPYSVIPKYIAFLSPDENYDEMVYQVYTGAYDAVVGDVTIVANRSLYVDFTLPYTESGVSMMVPLKDNKNTWVFLRPWSLDLWVTTACFFVFIGFIVWILEHRVNTDFRGPPHHQIGTSFWFAFSTMNFAHREKVVSNLARFVVLVWCFVVLVLIQSYTANLTSFFTVKLLQPTVTNWKDLIKFNKNIGYQRGTFVRELLKSQGFDESQLKPFGSAVECDELFSNGTITASFDEVAYIKVILSQNSSKYTMVEPSFKTAGFGFVLEVWLSIVAALVGTAIMIWFMDKYSPYSSRNNRQAYPYACREFTLRESFWFALTSFTPQGGGEAPKAISGRMLVAAYWLFVVLMLATFTANLAAFLTVERMQTPVQSLEQLARQSRINYTVVKDSDTHQYFVNMKFAEDTLYRMWKELALNASKDFKKFRIWDYPIKEQYGHILLAINSSQPVADAKEGFANVDAHENADYAFGRDRSGLPIWEGYCIDFIIRLSQKLNFEFEIVAPEVGHMGELNELGEWDGVVGDLVRGETDFAIAALKMYSEREEVIDFLPPYYEQTGISIAIRKPVRRTSLFKFMTVLRLEVWLSIVAALVGTAIMIWFMDKYSPYSSRNNRQAYPYACREFTLRESFWFALTSFTPQGGGEAPKAISGRMLVAAYWLFVVLMLATFTANLAAFLTVERMQTPVQSLEQLARQSRINYTVVKDSDTHQYFVNMKFAEDTLYRMWKELALNASKDFKKFRIWDYPIKEQYGHILLAINSSQPVADAKEGFANVDAHENADYAFIHDSAEIKYEITRNCNLTEVGEVFAEQPYAVAVQQGSHLGDELSYA"
  },
  {
    "reference": "MQYPEPIARLIDSYMKLPGIGEKSATRLAFYTLGMSDADVDNFAKSLRAVKRDLHYCSVCGNITEDDPCPICKDKTRDQSRVLVVERSRDIMAMERMKEYHGLYHVLHGTISPSEGTGPQDINLESLLKRLEAHKEIQEVIVATNASLDGETTAQYLAHLIKPAGIKVTRLAHGLSAGADIDYTDEVTLFRAVQGRTEM",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: It seems to be involved in an RecBC-independent recombinational process of DNA repair; It may act with RecF and RecO..2. The designed protein must be able to regulate DNA recombination, DNA repair. The designed protein sequence is ",
    "response#1": "ALLCVVETPGDQLMIEQTLTFKGLYFVLMGRLSPLDGIGPKDIHLEKLISRATDGIVQEVVLATNFTNEGEATAHYISETLKARGLKVSRLARGVIG",
    "response#2": "KLMIVTTNEDLERIESANIYNGLYHITNGEISLRKNVVIEHTNIKTIKERVLNGSFKEIIIATSYTHDGEVTADYIIRMLEDIKDLQIYRV",
    "response#3": "LLCVVETPGDQLMIEQTLTFKGLYFVLMGRLSPLDGIGPKDIHLEKLISRATDGIVQEVVLATNFTNEGEATAHYISETLKARGLKVSRLARGVPVGGELEYVDAGTIARR"
  },
  {
    "reference": "MTHEILTPAEMGEADRLTIATGPGDGYGLMLNAGTAIASHLLTHHGDAAESHILCGPGNNGGDGYVVARILAESGARVQVWSSGAPKSGTDAARALQVCPVRPRPIDEFEPTSGSLIIDALFGAGLDRPVTGKAAAAIARANDAPTYRLSIDIPSGLDGASGQILGTVFNADCTITFFRKKPGHLLYPGRKLCGDLIVADIGIRENVLAAIKPSCFENTPDLWRTFLPAPDAATHKYERGHVAVFSGGATSTGAARLSALGAARSGAGAVTVLSPDEALAVNAAHLTSIMLSRCDHPLDLESFIEKRRPSSFVLGPGFGIGDKTRAFGLALFRDLTARLVLDADAITSFRDHTDNLFSAARSAPQVRLVVTPHEGEFKRLFPDLVESATPSKLERARTAAARANAIIIYKGADTVIASPDGRAAINTNGTPLLATAGSGDVLAGLAAGLMAQDMPPFEAACAAVYIHGAAARHLGCGLIAEDLPAAAGKVIGGLIGALVDQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.2. The designed protein should have a high specificity for (6S)-NADPHX, K(+), AMP binding, with minimal off-target effects.3. The protein contains novel YjeF N-terminal domains that confer a unique function or activity.4. The designed protein must possess ATP binding, NADHX epimerase activity, metal ion binding, ADP-dependent NAD(P)H-hydrate dehydratase activity to support nicotinamide nucleotide metabolic process under certain conditions.5. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "MRKIDSYCINELGISELILMENAALKIINNIDLKNNMSFTVVCGSGNNGGDGLAVARHLISLGKAVQVFFVGNIDKMSDSCKVNYLILKNMKINIINIISLEYIEALNLAVARSDITIDALLGTGISRDVQGIHTLVISVINENTNFTLAIDVPSGLNSNNGNKMGNCIISNKTVTFEFYKSGFLNYDSEKYTGEIIVEKIGIPQFVIKTTALSTLPLPRPPVTHKYKEGHLLLICGSRRYSGGALLTGLGGRASGVGMLSIAVPESLKSIMVAQLPEALIIGCPETESGAIAQLQLPENTDLSKFNAIACGPGLTRDANPILQQVLESTVPLVLDADGLNILADMGTIPTLQKRQAPTVLTPHAGEFKRLFPDLSDANQDRVKAVREAAAQSHAVVLLKGARTAIANPQGTVWLNPESTPALARGGSGDVLTGLLGGLLAQASSKNIPVEDITATAAWWHAQAGILAAQERTELGVDAHTLTQYLIQVLDSNEAKVMDKLTIESGIMEETLMEQAAFAIADVASSFSPKKILCVAGTGNNGGDCIAAGRILHNKGYNVEIYIYGKNQSNGFKKQLLIAENYGLKIKYNKDFSDEYDLIIDGLIGIGLNGEVRSSIKTVIEKINSSNAKVISEDIPSGISSDTGEVMGDAVHADITITFGNLKIGQILYPGREFSGKLKIAELSFKDSHKYDYGNVLILAGSSKYPGAPILTALGAQRIGAGLVTLITPGDSSKILSFEPSIIYSSLNKNNFEKNDLKKLNDEIERANVIILGPGMTEDSIEFVVELIKKQGRTKNIVLDAESIQAIKEFESLSENILITPHVGEMKKIFKNLKNNIFELERFSKQKKCIIMLKSSITTITDGEKTFFNLLGSSSLSKGGSGDLLSGIVGGLIAQGLDTINACITGSYIMYKTAKELSNEYTEYFITPKLIS",
    "response#2": "MRKADASVIRSGTPGVTLMQNAGAAVADAAREMVSPGARIVVLAGPGQNGGDGIVAATRLREQGYRVSLGLLGKREAFSGDAALVAREWPSDVVAVDGANLAPAELVIDALFGGGLSREIEGAARAAIDRINMSGGPVLAVDLPSGIDGETGAVRGIAVRAARTVTFAARKPGHYLLPGRTFCGDVTVADIGVGSSDLDASVAGAGDVLTGVHAQASHKGSFGDVAVIGGQSTATSATHMTGAALLAARAALHAGAGRVFVALLGTDGPRVDAQQPELMFRAPEALDLKKQVVVCGCGGGEAVKAVIARVLSSATRAVLDADALNAIATDPQLQTQLKARHGRGYSTVLTPHPLEAARLGGMTAAAVQADRLATAEQLAQRFQCVVVLKGSGTVIAGPGQTSMINSSGNALLATAGTGDVLTGMIAACLARGMNDFEAACCAVFTHGHRAEIWAIDCPGQTLTASALRDAHKGNFGHVLVIGGDVGFAGAPLLAGEAAARVGCGLVTLATRPQHAGMQVAARPELMAWGVDRPAQLDPLLDRASVVAIGPGLGQSPWAAGLLARVLETNLPLVVDADALTLLARDPVQNERWVLTPHPGEAARLLDCSTAEIQGDRFTAVRELQSRYGGAIVLKGCGSLVATRKSGVTVCTDGNPGLASGGTGDVLTGVIAGLMAQGLEPESAALTGACLHGAAADRAVLEGERGMLAGDLR",
    "response#3": "MRKIDSYCINELGISELILMENAALKIINNIDLKNNMSFTVVCGSGNNGGDGLAVARHLISLGKAVQVFFVGNIDKMSDSCKVNYLILKNMKINIINIISLEYIEALNLAVARSDITIDALLGTGISRDVQGIHTLVISVINENTNFTLAIDVPSGLNSNNGNKMGNCIISNKTVTFEFYKSGFLNYDSEKYTGEIIVEKIGIPQFVIDHKGRRGHALVIAGSPGKWGAGRMAARAALRAGAGLVTLAGPRADAAAPDEVMTAAIDPEAAGFDVALAELLAGKRAVAIGPGMPATAAGAALVRAVLERAEVPVVIDADALNHMAGDLAAVAAARAPVVLTPHPGEAGRLLGCSAADIERDRIAAVRRLAERTRAVVVLKGARTLVCDGAVDGFTTVNPTGNAAMATAGAGDVLTGVIVALAAQGAPPADAARAGVFLHGLAGDRAARGRIDAGLMAGDLIDELL"
  },
  {
    "reference": "MTMTDPIADMLTRVRNASRAYHDRVVMPHSKIKTHIAEILQQEGYISGWHVEDATSAGGVGHTLVIDLKYGPNRERSIAGIKRISKPGLRVYAKATNLPKVLGGLGVAIISTSSGLLTDKQAGKRGVGGEVLAYIW",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have rRNA binding, structural constituent of ribosome within ribosome to regulate translation. The designed protein sequence is ",
    "response#1": "ERGNTITFGEYGLVALEPAWITNRQIEAARIAMTRYIKRGGKVWIKIFPDKPITAKPAETRMGSGKGSPEYWVAVVKPGRVMFELAGVPEEIAKEALRLAMHKLPVKCKIT",
    "response#2": "MTRGNTITFGEYGLVALEPAWITNRQIEAARIAMTRYIKRGGKVWIKIFPDKPITAKPAETRMGSGKGSPEYWVAVVKPGRVMFELAGVPEEIAKEALRLAMHKLPVKCKIS",
    "response#3": "MALKIKKGDKVVVLAGKDKGKRGEVLKAIPN"
  },
  {
    "reference": "MAKIYATGKRKTAIAKVWLTPGSGKLTVNGTTLNDWLGGHEAIKMKVMQPLLLTKQEKSVDIVSVTLGGGYSAQAEALRHGISKALSAYDIAFRAILKPKGLLTRDSRVVERKKYGKRKARRSPQFSKR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that localizes to ribonucleoprotein complex, ribosome and can perform structural constituent of ribosome with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "DFPIEMQLIAMESCQVRHTALEAARVSVNRRMTEAAGLDNFYLKVVPYPHHVLREHKMATGAGADRISSGMRAAFGRPVGTAARVYQNDVIMIGRTDEAHAHELKIALKKAAIKLPTPCKVI",
    "response#2": "DFPIEMQLIAMESCQVRHTALEAARVSVNRRMTEAAGLDNFYLKVVPYPHHVLREHKMATGAGADRISSGMRAAFGRPVGTAARVYQNDVIMIGRTDEAHAHELKIALKKAAIKLPTPCKVI",
    "response#3": "DFPIEMQLIAMESCQVRHTALEAARVSVNRRMTEAAGLDNFYLKVVPYPHHVLREHKMATGAGADRISSGMRAAFGRPVGTAARVYQNDVIMIGRTDEAHAHELKIALKKAAIKLPTPCKVI"
  },
  {
    "reference": "MNQIKNKTLPEMTTEQALLSMEALPLGFRFRPTDEELINHYLRLKINGRDLEVRVIPEIDVCKWEPWDLPGLSVIKTDDQEWFFFCPRDRKYPSGHRSNRATDIGYWKATGKDRTIKSKKMIIGMKKTLVFYRGRAPRGERTNWIMHEYRATDKELDGTGPGQNPYVLCRLFHKPSDSCDPAHCEEIEKVNFTPTTTTRCSPDDTSSEMVQETATSGVHALDRSDDTERCLSDKGNNDVKPDVSVINNTSVNHAETSRAKDRNLGKTLVEENPLLRDVPTLHGPILSEKSYYPGQSSIGFATSHMDSMYSSDFGNCDYGLHFQDGASEQDASLTDVLDEVFHNHNESSNDRKDFVLPNMMHWPGNTRLLSTEYPFLKDSVAFVDGSAEVSGSQQFVPDILASRWVSEQNVDSKEAVEILSSTGSSRTLTPLHNNVFGQYASSSYAAIDPFNYNVNQPEQSSFEQSHVDRNISPSNIFEFKARSRENQRDLDSVVDQGTAPRRIRLQIEQPLTPVTNKKERDADNYEEEDEVQSAMSKVVEEEPANLSAQGTAQRRIRLQTRLRKPLITLNNTKRNSNGREGEASHRKCEMQEKEDISSSSSWQKQKKSLVQFSSVVIIVAVIVVLVEIWKESRDAKCSFLFHQLDSFKGMFT",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein with DNA binding capability for targeted gene regulation.2. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.3. The designed protein must be able to perform DNA-binding transcription factor activity under specific conditions.4. The designed protein should contain one or more NAC domains that are essential for its function.5. For general function, the protein need meet that Transcriptional activator activated by proteolytic cleavage through regulated intramembrane proteolysis (RIP). The designed protein sequence is ",
    "response#1": "PSLPALDWQLPSHSGPYELRIEVQPKSHHRAHYETEGSRGAVKASAGGHPSVQLHGYVESEPLTLQLFIGTADDRLLRPHAFYQVHRITGKTVSTASHEAVVCSTKVLEIPLLPENNMRATIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHIPQPNGRTLSLQVASNPIECSQRSR",
    "response#2": "LRIEVQPKSHHRAHYETEGSRGAVKASAGGHPSVQLHGYVESEPLTLQLFIGTADDRLLRPHAFYQVHRITGKTVSTASHEAVVCSTKVLEIPLLPENNMRATIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHIPQPNGRTLSLQVASNPIECK",
    "response#3": "PSLPALDWQLPSHSGPYELRIEVQPKSHHRAHYETEGSRGAVKASAGGHPSVQLHGYVESEPLTLQLFIGTADDRLLRPHAFYQVHRITGKTVSTASHEAVVCSTKVLEIPLLPENNMRATIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHIPQPNGRTLSLQVASNPIECSQRSL"
  },
  {
    "reference": "MSDTITFVTGNSNKLREVVHILSHGAPPSNDGSHKVGKFTVVNNSLDLEEVQGTIEEVTIFKAKKAAELLQRPVLVEDTCLGYDALNGLPGPYIKWFVESLGLEGIVKLLAGFDDKSAKAYCTFGYCKGPGEDVVLFQGVTEGTIVLKARGPTDFGWDAVFQPKGYTETYAEMSKETKNLISHRYRALDKVRTFLLEQSE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein with metal ion binding, XTP diphosphatase activity functions is required to enable nucleotide metabolic process.2. For general function, the protein need meet that Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.3. The designed protein should be able to compete with endogenous proteins or inhibitors for ITP, Mg(2+) binding. The designed protein sequence is ",
    "response#1": "GSRNPVKINATKEALDVLKLNWDLIGIEVDSGVDKQPFCDQTYVGARNRALNVIRVTNADIGLGIEGGVCNVYGKFIANAVVYVITKEGLENFAISSSFTLPSSMVSLILQGKELGEASDIIFKTNNSKTKEGAIGLLTNNVINRKMLYVQPIVLALYPK",
    "response#2": "GSRNPVKINATKEALDVLKLNWDLIGIEVDSGVDKQPFCDQTYVGARNRALNVIRVTNADIGLGIEGGVCNVYGKFIANAVVYVITKEGLENFAISSSFTLPSSMVSLILQGKELGEASDIIFKTNNSKTKEGAIGLLTNNVINRKMLYVQPIVLALYPK",
    "response#3": "GSRNPVKINATKEALDVLKLNWDLIGIEVDSGVDKQPFCDQTYVGARNRALNVIRVTNADIGLGIEGGVCNVYGKFIANAVVYVITKEGLENFAISSSFTLPSSMVSLILQGKELGEASDIIFKTNNSKTKEGAIGLLTNNVINRKMLYVQPIVLALYPD"
  },
  {
    "reference": "MILGVSSGIAIYKAVDLSSKLRKSNANLSVVLTENAKKLVSPVVFSAVGNCPVYTDLNDVNNGWIPHTQLSMNADALIIAPATANIIAKIANGFADDLLSLIALAFRKKAKILVPTMNTRMYESPALKRNLETLKNDGWLIVEPEVGHLACGEIGKGRYPENNMILEFLEYSLTKKDMKGKNVLITAGPTIESIDPVRNITNKSSGKMGYELARAFSYRGAEVTLISGPTHIKPPYILKKFIKVNTAFEMKNEVMKLYKDFDIIIMAAAVSDFKVMNYSKEKIKKDLSNFSLELIKNPDILKELGKIKNKNQFLVGFAAESNNLIDYAKKKLIEKNCDLIVANDISKKEIGFDSELNEIYIINKMGDVTHVEKNSKKYIAFRIIDYISDNYNGKS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein should be able to manipulate the activity of enzymes in the CoA from (R)- pantothenate: step 3/5 pathway, either by activating or inhibiting their function.2. The designed protein should have a binding site that is specific to the target CTP ligand.3. The Proton donor active site of the designed protein must be optimized for substrate binding and specificity.4. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A5. The protein contains novel DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains that confer a unique function or activity.6. The protein design must be able to perform metal ion binding, phosphopantothenoylcysteine decarboxylase activity in order to contribute to pantothenate catabolic process, coenzyme A biosynthetic process. The designed protein sequence is ",
    "response#1": "MTIVVGVTGGIAAYKAVSVIRSFVLLGHDVHVIATDAALRFVGAPTFEAISRNPLNTDLYEGVSEVRHVGIGKSADLVVIVPATANTLAKIANGLADDLLTNTILATTAPIVVAPAMHTEMWQNKATQANVQTLRSRGVTLVGPATGQLTGSDSGPGRLEEPEVIVEAALAVLGPAGRGTRVLVTAGGTREPVDPVRYLGNRSSGRMGFALAAAARDRGAEVTLVAGPTELATPEGVTRHDVTTALEMHAAVFEHLAAADVVIKAAAVADFRPGSPATSKLKKEHGVPRIELTPNPDILAELGAGRTGDHPLLVGFAAETDEVEAGGWAKLERKGADLLVVNDVAADDAGFEVEHNRVVILRRGREPVVVPLGPKDRVADAVWDLVVPLLR",
    "response#2": "MTIVVGVTGGIAAYKAVSVIRSFVLLGHDVHVIATDAALRFVGAPTFEAISRNPLNTDLYEGVSEVRHVGIGKSADLVVIVPATANTLAKIANGLADDLLTNTILATTAPIVVAPAMHTEMWQNKATQANVQTLRSRGVTLVGPATGQLTGSDSGPGRLEEPEVIVEAALAVLGRRSDLAGHRIIVTAGGTREPLDPVRFLGNRSSGKQGVAIATAARDRGADVVLIAAHLDVPVPDGLETRSVSSALELQKAVTDAASGADVVVMAAAVADFRPAKLQEGKIKKDDVGEGLQLSLVANPDILAGLSAGKRPDQVIVGFAAETESDPQALIELGRRKLKRKGCDFLVINNVGWKTGFSTDDNEVVVLAPDGSREELPRQSKIALAHVVWDEVARRLPP",
    "response#3": "MTIVVGVTGGIAAYKAVSVIRSFVLLGHDVHVIATDAALRFVGAPTFEAISRNPLNTDLYEGVSEVRHVGIGKSADLVVIVPATANTLAKIANGLADDLLTNTILATTAPIVVAPAMHTEMWQNKATQANVQTLRSRGVTLVGPATGQLTGSDSGPGRLEEPEVIVEAAELLLERPDDLPADLSVVITAGPTREAIDPVRYISNHSSGKMGFAIAEAFAKRGAKVTLIAGPVNLVTPPKVCRVDVISAQEMAEQAVSLAQQNAIFIGCAAVADYRVEQVAEQKIKKTDDSDELTLKLVKNPDIIANVAHLTENRPFVVGFAAETENVAEYAKSKLQRKKLDLICANDVSGGQVFGQDQNALHLLWADGATELPRMAKTELARQLLTLIAQRYDEKNA"
  },
  {
    "reference": "MQESISLCLRGGEPQAQDFGGIESKFALRTVKEPDEDTCYLVPGQEDTVAKCNFNHTSKTFVVIHGWTVTGMYESWIPKLVYALYKREPESNVIVVDWLRRAQQHYPVSAAYAQQVGEDIATFVDWMEEQFNYQLDNLHLLGYSLGAHAAGIAGSLTKTKINRITGLDPAGPNFEYAEANTRLSPDDAGFVDVLHTYTRGSPDRSIGIQKPVGHIDIYPNGGGFQPGCNLGETIRLLAERGFGDVDQLVKCSHERSIHLFIDSLLYEESPSLAYRCSTKETFEKGLCLSCRKNRCNSLGYNINKVRTKRSTKMYLKTRSQMPYKVFHYQVKIHFFGKLNVTKTNQPFLLSLYGTLDESENIAFILPEISTNKTASFLIYTEVNIGDLLMLKLQWENDSIFSWSNWWINPEFRIQRVRVKAGETQKKLVFCSRDGISHLKKGKEAVVFVRCTDKFMNV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have apolipoprotein binding and be active in catalytic complex.2. For general function, the protein need meet that Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans. The designed protein sequence is ",
    "response#1": "CVCTGSSTRHIVTFDGQNFKLMGNCSYVLFHNKEQDLEVILHNGACGAGARQACMKSIEVKHNGLSVELHRDMEVVVNGRQVSVPYVGGNMEVGIYGTIMYEVRFNHLGHILTFTPQNNEFQLQLSPKTFASKMYGLCGICDENGANDFMLRDGTVTTDWKTMVQEWAVQQPGQMCS",
    "response#2": "DLVFLLDGSYRLSEAEFEVLKAFVVGTMERLHISQKRIRVAVVEYHDGSHAYLELRARKRPSELRRIASQIKYVGSQLASTSEVLKYTLFQIFGKIDRPEASRVILLLTASQEPQRMARYFTRYLQGFKKKKVILIPVGIGPHANLKQIRLIEKQAPENKAFLLSGVDELL",
    "response#3": "ASCSALGLAHYLTFDGLKYLFPGECQYVLVQDYCGSNPGTFRILLGNEGCGYPSLKCRKRVTILVDGGEIELFDGEVMVKKPLKDETHFEVVESGRFITVLLGSGLSVVWDRHLGISVFLKQTYQEQVCGLCGNFDGVQNNDLTGSSLQVEEDPVDFGNSWKVSPQCR"
  },
  {
    "reference": "MSPSDDPISSIFFERQQAALCAQHALNMLLQDSLFTYENLRDLARQMDQMEHDILGNNANAVGRSENMNDSGFFSIQVIEKALETFDLKLINMENPAMAEFKANPLTARAYVLNLREHWFVLRKFGNQWFELNSVKNGPKLLTDTYVKEYLHQFAAENYSIFVVQGILPNSEADDFITLCPVVPKPTDFDKKEPNLVQKFFNSVGRRLGGSQEIPDSQEDRDLAIAMALSMESKESSESSGSDEDQLAKAIEMSLSQDPNIPSTSAAPSELTETPILGPSTSSETPSGRIPSAEQQRRDRAKFLEKLEEEKKSQNVPEE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein's functionality will benefit from Polar residues, Basic and acidic residues compositional bias in its structure.2. The presence of UIM, Josephin domains is critical for the interaction of the designed protein with its intended targets.3. A protein that contributes to chemical synaptic transmission, protein deubiquitination by functioning in cytoplasm, nucleolus, synapse with cysteine-type deubiquitinase activity.4. For general function, the protein need meet that Regulates germline DNA double-strand-break repair and apoptosis in response to DNA damage by recruiting E4 ubiquitin-protein ligase ufd-2 to DNA repair foci; Probably by regulating the IGF-1-insulin-like pathway, regulates lifespan; Acts as chain editing deubiquitinating enzyme that binds and cleaves 'Lys-48'-linked polyubiquitin chains, with a preference for chains containing four or more ubiquitin molecules thereby modulating protein degradation by the ubiquitin-proteasome pathway; Acts as a histone-binding protein that regulates transcription.5. The design of the protein must focus on the precise arrangement of amino acids in the active site, Proton acceptor, Nucleophile to enable effective catalysis of Thiol-dependent hydrolysis of ester, thioester, amide, peptide   and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-   residue protein attached to proteins as an intracellular targeting   signal).. The designed protein sequence is ",
    "response#1": "MEPILINAQVQMWSAKAGMSKSRNALIETCVGKREVKLILYFSTGKIKTLQLHDNIKSVVLQTYGEDQNYLHLTFKNNDFLFVEKLTTTDARRLKRFLDKTSLPCPIRPSSSSPGLSNLGNTCFFNAVMQNLSQTPVLRELIKEVRMSGTIVKIEPPDLALTEPLEVNLEPPGPLTLAMSQFLNEMQETKKGIVTPRELFSQVCKKAVRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEEVKNKVKDYEKKKPVPSFVDRIFGGELTSTIMCDECRTCGKKSINDKNLKKIMEEEDKDSEDEKDDDSHMKARNDILSGTSKHLQKKAKKQAKKQAKNQRRQQKIQEKVLHLNNICATDHTENECKAEMSPVGEVDIDSNHISQEEVTHTEIDVNLKDLNGQEKTIENTTDNQNSPEELGVSGVSMDSALEVLTSDRNLNGVCLEERSSGELDLTSDFKNLNLNAALHPDEINIEILNDSHDLGTKVYEVVNEDPETAFCTLANREAFSTDEYSIQHCLYQFTRNETLQDANKLLCEACTRRQCNGPKANIKGERKHVYTNAKKQMLISLAPPVLTLHLKRFQQAGFNLRKINRHIKFPEILDLAPFCTLKCKNVAVESTRVLYSLYGVVEHSGTMRSGHYTAYAKTRTAR",
    "response#2": "MEPILINAQVQMWSAKAGMSKSRNALIETCVGKREVKLILYFSTGKIKTLQLHDNIKSVVLQTYGEDQNYLHLTFKNNDFLFVEKLTTTDARRLKRFLDKTSGSVTESPKPKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGICEPYEFVSLEWLQKWLDESTPTKPIDNNACLCSHDKLHPDKISIMKRISEYAANIFYSRYGGGPRLTVKASPKGLSNLGNTCFFNAVMQNLSQTPVLRELIKEVRMSGTIVKIEPPDLALTEPLEVNLEPPGPLTLAMSQFLNEMQETKKGIVTPRELFSQVCKKAVRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEEVKNKVKDYEKKKPVPSFVDRIFGGELTSTIMCDECRTCGKKSINDKNLKKIMEEEDKDSEDEKDDDSHMKARNDILSGTSKHLQKKAKKQAKKQAKNQRRQQKIQEKVLHLNNICATDHTENECKAEMSPVGEVDIDSNHISQEEVTHTEIDVNLKDLNGQEKTIENTTDNQNSPEELGVSGVSMDSALEVLTSDRNLNGVCLEERSSGELDLTSDFKNLNLNAALHPDEINIEILNDSHDLGTKVYEVVNEDPETAFCTLANREAFSTDEYSIQHCLYQFTRNETLQDANKLLCEACTRRQCNGPKANIKGERKHVYTNAKKQMLISLAPPVLTLHLKRFQQAGFNLRKINRHIKFPEILDLAPFCTLKCKNVAVESTRVLYSLYGVVEHSGTMRSGHYTAYAKTRTANSHLSNLVLHGEIPQGFEMES",
    "response#3": "MEPILINAQVQMWSAKAGMSKSRNALIETCVGKREVKLILYFSTGKIKTLQLHDNIKSVVLQTYGEDQNYLHLTFKNNDFLFVEKLTTTDARRLKRFLDKTSGLLLNENGDGNCLMHAASQYMWGIPDTDLVLRKALFSTLKETDTRNFKFRWQLESLKSQEFVETGLCYDTRNWTEEWDTLIRMASADTPGARGGLQYSSLEEIHIFVLCNILRRPIIVISDKMLRSLDSGSNFAPLKVGGIYLPLHWPAQECCRYPIVLGYDSHHGEWQLVALKTNGDGNCLMHAASQYMWGIPDTDLVLRKALFSTLKETDTRNFKFRWQLESLKSQEFVETGLCYDTRNWTEEWDTLIRMASADTPGARGGLQYSSLEEIHIFVLCNILRRPIIVISDKMLRSLDSGSNFAPLKVGGIYLPLHWPAQECCRYPIVLGYDSHHFVPLVTGNYTRAKLRKCTGLRNLGNTCYINSMIQCLFAAKTFRTLFISSKYKSYLQPIRSNGSHYSPKLSNSLSMLFNKMYLNGGCSVVPTGFLKVINQLRPDLKIPDDQQDTQEFLMILLDRLHDELSDQQHVANDYPNLLLYNADALKVSNNEYKHWFDKNVIGNGISPIDDIFSNHLSLMDVKCETPNCPFFMSVNTQPLCHECSERRRRGPWRQEGSLCAQHCLNNLLQGEYFSPVELSSIAQQLDEEERMRMAEGGVSSEEYRTFLQQPSVNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRLGIDPINEKSFICNYKEHWFTVRKLGKQWFNLNSLLMGPELISDTYLALFLAQLQQEGYSIFVVKG"
  },
  {
    "reference": "MGKKVNLKSFSKDELFHFIEVAGLPRFRAGQLIHWIYQRYATDISCITELSKELRSGLERMAYISDLKLLRRLKSSDGTEKFLFSLEDAETIESVLIPDDARLTLCISSQVGCAMGCKFCLTGKLGLIRNLRAYEIADQVIAVNRLILPQKITNIVMMGMGEPLANLDEVAEALWRITEFIDISKRRITLSTAGLVPRMALLADRAPHVNLAVSLNASSDKVRDDIMPINRRYPLKALIEACKKFPLGPRRRITFEYVLIGGVNDSPEDAGRLVKLVRGLRCKINLIPLNPHPESGMKRPSDAVVLKFQKALVDSGLTAFIRESKGQDILAACGQLRAGH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must have high specificity towards metal ion binding.3. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. The designed protein sequence is ",
    "response#1": "SSQAGCAVNCSFCSTGKQGFSRNLNTAEILGQIWLANQLLRQPGAQPRWNGSDDMARMDEDLDDGGAERPISNIVFMGMGEPLLNYGALLPALRALLDDHAYGLSRRRVTVSTSGVVPLIDRLSDDCPVALAVSLHASNDTLRDQLVPLNRKYPLKELLAACQRYLKVAPRDFITFEYVMLNGINDTVR",
    "response#2": "RPGLSGVEPRDFAAWFAERGQPAYRARQVADQHWSGRVERFTDMRTLPEAVRLALDDAFRLQTLAETTVRPTDDGLTDKALHGLDDGRSVESVLMRYPARGPRRARATVCISSQAGCAVGCPFCATGELGFWRDLSAAEIVDQVRFWAARLARDGERVTNVVFMGMGEPLLNTDAVISAATALTDPRRFGLGARHVTISTSGVVPGIERLTELKPQWTLAVSLHAARAALRDVLVPLNRRWSVDRVVAAAAAYTAATGRRVTYEYVMIDGINDTPTDAADTAALLGGHLAHVNLIPMNPVAHTPWRPSGRDRIEAFAAILRGAGLATTVRRNRGIEIGAACGQLAAERAAG",
    "response#3": "RPGLSGVEPRDFAAWFAERGQPAYRARQVADQHWSGRVERFTDMRTLPEAVRLALDDAFRLQTLAETTVRPTDDGLTDKALHGLDDGRSVESVLMRYPARGPRRARATVCISSQAGCAVGCPFCATGELGFWRDLSAAEIVDQVRFWAARLARDGERVTNVVFMGMGEPLLNTDAVISAATALTDPRRFGLGARHVTISTSGVVPGIERLTELKPQWTLAVSLHAARAALRDVLVPLNRRWSVDRVVAAAAAYTAATGRRVTYEYVMIDGINDTPTDAADTAALLGGHLAHVNLIPMNPVAHTPWRPSGRDRIEAFAAILRGAGLATTVRRNRGIEIGAACGQLAAERAAAA"
  },
  {
    "reference": "MTGKIDLKNLTLPALEQFLQGQGKERFRALQVFKWIYQQDAGGFEEMTNISKALRTELAETAFISNLEPEAVEEGSDGTRKYLFNLGDGHAVESVLIPIEGRNTLCISSQVGCAMACEFCLTGTFKLTRNLTTAEIINQIMAVKRDIARNPPSLLVEQNEFDDADESDDGHPDSPAAIRNIVLMGMGEPLHNLDNVIPAIQIMIDGNGLQLSNRRVTVSTCGLVPEMARLGREIPNVNLAVSLNATTNELRDRIMPVNRSYPIESLLQACREFPLPGRRKVTFEYVMLGGVNDSLDDAKRLLRLISDIPNKVNLIPFNEHDGCGFKAPSQKAIDAFHKYLIDRHVTVITRDSRGGDISAACGQLKGKLEKK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The desired protein should be able to perform adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine2. For general function, the protein need meet that m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.3. The designed protein should be expressed specifically in the cytoplasm4. The protein should be able to bind S-adenosyl-L-methionine ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MGQHYTQCLSTQVGCAMACTFCSTGELGLTRNMTMGEILGQVVVGRAHLAASGLPTVRNLVFMGMGEPLMNLAELLRSLETLGSAEGISIASRRATVSSVGIPQALKILGDSGLAVPAISLHAPTQELREKLMPKAAHVPLDELLGCLDRYPLKSRERVTYEYILLGGVNDSIVHAKQLVKLLGQRRCKVNLIGYNPPPPSVCPDPPYRAPEQADVLAFEEYLRSKRITTFIRHPLTLKPGEIPVNLLGLELDALVALCEELGEKRFRAVQLFRWVHQRGVSDFGQMTDLAKSFRAKLAERAYVATAAITHQHESRDGTIKWLFDVGGGDAIEAVFIPEEGRNTLCVSSQAGCAVGCRFCFTGHQGFSRNLSSAEIVGQLWYAEHFLRKRLGGAERVISNVVFMGMGEPLQNYRNLVPALRILLDDHGYGLSRRRVTVSTSGVVPMIDRLAQDCPVALAVSLHAPDDTLRDYLVPLNRKHPIDELLRSCIQYLQYAPRDFLTFEYCMLDGVNDLPQQARALVELVRAYGMQGLRCKFNLIPFNPFPGSGLIRSTDENIRAFAQILADAGIVTTVRKTRGDDIEAACGQLAGSVQDHTHVLNWH",
    "response#2": "MGQHYTQCLSTQVGCAMACTFCSTGELGLTRNMTMGEILGQVVVGRAHLAASGLPTVRNLVFMGMGEPLMNLAELLRSLETLGSAEGISIASRRATVSSVGIPQALKILGDSGLAVPAISLHAPTQELREKLMPKAAHVPLDELLGCLDRYPLKSRERVTYEYILLGGVNDSIVHAKQLVKLLGQRRCKVNLIGYNPPPPSVCPDPPYRAPEQADVLAFEEYLRSKRITTFIRNLEGRKP",
    "response#3": "MNDRYSQCLSTQVGCAMACTFCNTGKLGFERNLTYGEIMGQILVGRQYLEDQGLNPLKNLVFMGMGEPLLNLDTLIKVLTDLPCERGLSLSWRRSMVSTVGFPDKLKILGDLEIALPAISLHAPTQELRAKIMPKAAKVHLDDLMASLKVYPMRSRERITFEYLLLKGVNDSMEHADQLARLIDRRKGKINLIAYNATEGMPYDAPDRAQVEAFEKRLWDHGMTAFIRRSMGSETTVGALEALGLGPDALVNLLDLDADGLATYCGELGEKPFRARQLQRWIHQFGASRFDEMSDLAKSLRQKLATRAEIRAPSAITDHVSADGTRKWLLDVGNGNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLTTGEIIGQLWMAEFAMRAQLGRGPKDDRVISNVVMMGMGEPLLNYDQVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLARDLPVALAVSLHASNDALRDGLVPLNRKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDGVEHARELLKLVADVPCKFNLIPFNPFPESGLKRSNNEQIRRFAQVLIDAGIVTTIRKTRGDDIDAACGQLAAEGRVQDQLRTLD"
  },
  {
    "reference": "MRITFVCTGNTCRSPIAESIAKKMLVDDTINSRGLFAIDGQSVSPESLEVIMEHNLPEPTVAKQFSEKDLNSDLILTMTDMHKQQLVSHYGDNGRIYQLSEYVGEIGDIVDPFGGSIDTYRQTFEQLLYLIGKLRTNS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be able to perform protein tyrosine phosphatase activity under specific conditions.2. For general function, the protein need meet that Dephosphorylates the phosphotyrosine-containing proteins.3. The Nucleophile, Proton donor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "WMMIVEQKCRVIVMLAKCFEAGKKKCQKYWPDSEETKTFGRVKVFNADEVKYCGFLRRRFHIESFDENDVCGRVFQYQYINWPDHSVPNTTSNLVRMHKYVIQCLEEIGGDAPMVF",
    "response#2": "WMMIVEQKCRVIVMLAKCFEAGKKKCQKYWPDSEETKTFGRVKVFNADEVKYCGFLRRRFHIESFDENDVCGRVFQYQYINWPDHSVPNTTSNLVRMHKYVIQCLEEIGGDAPMVI",
    "response#3": "WMMIVEQKCRVIVMLAKCFEAGKKKCQKYWPDSEETKTFGRVKVFNADEVKYCGFLRRRFHIESFDENDVCGRVFQYQYINWPDHSVPNTTSNLVRMHKYVIQCLEEIGGDAPMVF"
  },
  {
    "reference": "MDYNRMNSFLEYPLCNRGPSAYSAHSAPTSFPPSSAQAVDSYASEGRYGGGLSSPAFQQNSGYPAQQPPSTLGVPFPSSAPSGYAPAACSPSYGPSQYYPLGQSEGDGGYFHPSSYGAQLGGLSDGYGAGGAGPGPYPPQHPPYGNEQTASFAPAYADLLSEDKETPCPSEPNTPTARTFDWMKVKRNPPKTAKVSEPGLGSPSGLRTNFTTRQLTELEKEFHFNKYLSRARRVEIAATLELNETQVKIWFQNRRMKQKKREREEGRVPPAPPGCPKEAAGDASDQSTCTSPEASPSSVTS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that contributes to rhombomere 5 development, positive regulation of transcription by RNA polymerase II, anterior/posterior pattern specification, pattern specification process by functioning in nucleus, cytosol, chromatin with DNA-binding transcription activator activity, RNA polymerase II-specific, DNA-binding transcription factor activity, RNA polymerase II-specific.2. For general function, the protein need meet that Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis3. The protein's architecture should predominantly feature helix-helix-helix-helix.4. The designed protein should contain at least one Homeobox domain that can specifically recognize and bind to DNA.5. Incorporating a Antp-type hexapeptide motif is critical for the successful design of the protein. The designed protein sequence is ",
    "response#1": "MRRYRTAFTREQIARLEKEFYRENYVSRPRRCELAAALNLPETTIKVWFQNRRMKDKRQRLAK",
    "response#2": "PDFSSLPSFLPQTPSSRPMTYSYSSNLPQVQPVREVTFREYAIEPATKWHPRGNLAHCYSAEELVHRDCLQAPSAAGVPGDVLAKSSANVYHHPTPAVSSNFYSTVGRNGVLPQAFDQFFETAYGTPL",
    "response#3": "PDFSSLPSFLPQTPSSRPMTYSYSSNLPQVQPVREVTFREYAIEPATKWHPRGNLAHCYSAEELVHRDCLQAPSAAGVPGDVLAKSSANVYHHPTPAVSSNFYSTVGRNGVLPQAFDQFFETAYGTPLLSRMLNLTDRQVKIWFQNRRMKEADIYIDTKDLCKRIAFELKNHSIPQAIFAERILCRSQGTLSDLLRNPKPWNKLKSGRETFRRMYNWVAQPLATRLAITREELAMKIE"
  },
  {
    "reference": "MAVTRDECQALDAEDPLKDFREEFSLPPGVIYLLGNSLGALPRRTPERVAHTVETEWGVHLGASWNSAGWWDLPQTTGDRIAPLIGARPGDVLAGDSTSVNIFKAVTAALPLRPGRRVIVSDLDNFPTDRYVVEGAARALGPYEIRDIGSAPGANGGTLEAALGDDVALVLLSHVDYRTGAMRDMAAVTAQVHAAGALMIWDLCHSAGVVPVEMGEVDLAVGCTYKYLNGGPGAPAYIYVAPRHQEAVHNVVSGWHGHAAPFAFEPSYRPAPGVRRFAVGSPPVLSYAALGASLDIWERVDMAAVRAKSVALTSLFIELTDELCPDLALVTPRDPAGRGSQVSYRHADGYPVVRALADRGVVGDYREPEIIRFGFAPLYLRHVDVYDAATTLAEVLGKELWRDERYSRRLAVT",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The presence of pyridoxal 5'-phosphate is necessary for the protein to function in the desired environment.2. A protein should be able to manipulate the activity of enzymes in the quinolinate from L-kynurenine: step 2/3, Cofactor biosynthesis, NAD(+) biosynthesis pathway, either by activating or inhibiting their function.3. The designed protein should have a high specificity for pyridoxal 5'-phosphate binding, with minimal off-target effects.4. The protein must exhibit the following characteristics: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively..5. The ideal protein would be one that plays a critical role in L-kynurenine catabolic process, quinolinate biosynthetic process, 'de novo' NAD biosynthetic process from tryptophan, anthranilate metabolic process, tryptophan catabolic process through pyridoxal phosphate binding, kynureninase activity within cytoplasm.6. The designed protein must have at least one Aminotransferase class V domain.7. The protein should have a fast turnover rate for H2O + L-kynurenine = anthranilate + H(+) + L-alanine. The designed protein sequence is ",
    "response#1": "MTLKTLLQNLKITTLIGNTXAXVXSAXDAAXXDXAXGFLXTNIRSXEXPXWXEXA",
    "response#2": "MSKKSFSFKKFLKKEYDKKLVXYXGXYXDXPXYXGXKSIXDXKXIAX",
    "response#3": "MSNRYKDRTSLIKLGIDEFSVAEKQGKLNPSFYLSCENLAELEKKYSNSDNFPTHLKKESKDIYLDLNLSEEKALEINPIMNSDTLFSSDNVSISGKKVFVCGMGGSGISGKLCSALLKDAGINTVVTNDNNFNQKNSDIYIGLSTSGNTKSTINLLKKAKHLGANTLGITFNADSPLANKSQYPLFVPNNYPENCSNFQHSHLLIVLAIIAQKYFGSDLISLRNNLKTAKETLSY"
  },
  {
    "reference": "MPIRIPDQLPASDVLRNENIFVMSESRASTQEIRPLKVLLLNLMPKKIETETQFLRLLSNSPLQVDIELLRIDDRPSKNTPEEHLNTFYRQFELVKNRNFDGLIITGAPLGLVQFEDVAYWQHLQNIMAWAKAHVTSTLYICWAAQAGLKLLYNLPKRTREEKLSGVYYHDIHKPFHPLLRGFDDRFLAPHSRYADFDAEFLAEHTDLDILATSDVAGVYLAATKDKRNVFVTGHPEYDAYTLHGEYVRDLGEGLNPAIPVNYYPNDNPDNKPCASWRSHGHLLFANWLNYCVYQQTPYDLEKFSEANFTKDE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must have high specificity towards homoserine O-succinyltransferase activity. The designed protein sequence is ",
    "response#1": "FGTSHRQKPVKDVVASVRSGLADLFSLPEGYEVVLGNGGTTAFWDAAAFGLIREKSLHLTNGEFSSKFASVAKNNPFIGDPIVVSADPGSAPEPVSDPSVDLIGWAHNETSTGVAIPVSRPAGSENALIAIDATSGAGGLPVNVADADVYYFAPQKCFAADGGLWIALMSPKALERVAEIKDSGRWTPDFLSLPIAVDNSSKDQTYNTPAVATLLLLANQIDWLNGKGGLDWATSRTADSSSRLYQWAEASEYATPFVTDPAHRSQVVGTIDFDDKIDAAQVAKILRANGF",
    "response#2": "NAVLVCHALNASHHVAGRYEGQDKSEGWWDNLIGPGKPLDTDRFFVIGVNNPGSCFGSTGPASTDPKTGRPYGADFPVVTVEDWVDAQARLLDALGIEQLAAVIGGSLGGMQAMSWTVRYPQRVRHAIVVASAPNLSAQNIAFNEVARRAIVTDPDFHGGHFYAHGVVPKRGLRVARMVGHITYLSDDVMASKFGRQTLNPELAYSTQDIEFQIESYLRYQGDKFSDYFDANTYLLITRALDYFDPAREFDGDLSRALAGATAKFLVVSFTTDWRFSPERSREIVKALVDNRRDVSYAEIDAPHGHDALARFY",
    "response#3": "FGTSHRQKPVKDVVASVRSGLADLFSLPEGYEVVLGNGGTTAFWDAAAFGLIREKSLHLTNGEFSSKFASVAKNNPFIGDPIVVSADPGSAPEPVSDPSVDLIGWAHNETSTGVAIPVSRPAGSENALIAIDATSGAGGLPVNVADADVYYFAPQKCFAADGGLWIALMSPKALERVAEIKDSGRWTPDFLSLPIAVDNSSKDQTYNTPAVATLLLLANQIDWLNGKGGLDWATSRTADSSSRLYQWAEASEYATPFVTDPAHRSQVVGTIDFDDKIDAAQVAKILRANGF"
  },
  {
    "reference": "MSRLKNKNILVAVTAGIAAYKVAILVRLLVKQEAQVKVIMTPDARSFVTPLTLSTLSKNSVEWKFFSDNGDWNNHVEFALWADYMIIAPCTANTLSAMANGTCNNLVLATYLSAKCPVIIAPAMDLDMYQHPTTKSNLEILEKNNVHIIPSESGELASGLVGEGRMAEPEHIVDFLDKLIEKDLPLFGKHVLISAGPTYENIDPVRFIGNYSSGKMGYSLAEASYLLGAKVTLVSGPSAEKVSHKEITVQKITSAQDMYTAMHNNFLEADITIMAAAVADYRPKEIAEQKIKKDDDTMIIELVKNPDILKSLGEIKTPDQILVGFALETNNEIAFAEKKLKKKNLDFIVLNSMRDKEAGFQKDTNKITILDRNGEQKSFETKSKKEVAFDILSYLITQKLS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should contain a Helical membrane-spanning region.2. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.3. Ideal protein should have a binding affinity for key enzymes in the coenzyme A biosynthesis, Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway to enhance the overall efficiency of the pathway.4. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.5. The presence of Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains is critical for the interaction of the designed protein with its intended targets.6. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.7. The designed protein should be involved in pantothenate catabolic process by localizing to membrane and exhibiting phosphopantothenate--cysteine ligase activity, FMN binding, metal ion binding, phosphopantothenoylcysteine decarboxylase activity.8. The protein should be designed to catalyze the following reaction H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2. The designed protein sequence is ",
    "response#1": "KNILIGVTGGIAAYKIPSLVRLLKKAGAEVQIVMSPMSKEFVTPLTLSTVSGRPVLSEFFHKETGEWNSHVELGMWADVFLIAPATASSMGKMVNGIADNLLITTYLSAKCPVIFAPAMDLDMYKHPSTTKNVSELVARGHILLEPNHGELASGLCGEGRMQEPDQIMKVIVDQPDLGRLVTPLEGVNVMVTAGPTRERLDPVRYITNDSSGKQGLAIANAAKSKGATVTLIAGPNVEPTNPGIFRIDVESAIEMNNAVQERLKETDLFIGVAAVADYRPANFLKEKIKRHGNNSLTNLELTENPDVIANVAKVKNRPIIVGFAAETNDPLKHAREKRIRKGIDAIIVNDVSDPSIGFNSTENAATLIAWEGGSQELDRMPKLELAHALVERIVDLYR",
    "response#2": "MNIVLGVTGGIAAYKAAHVVRLLKEAGHSVRVVPTESALCFVGAPTWEALSGQPVQTDVFTRVEEVEHVLIGQQADLVLIAPATADSLARIRAGMADDLLTATVLTTTAPVVVAPAMHTEMWFNPATVDNVAVLRARGVHVLEPASGRLTGADSGPGRLPEPQDIVDFATTLVAKLGPSDSKLLISAGPTREALDPVRFLTNRSSGKMGFALAEEALARGAEVTLVSGPVALSTPRGARRIDVESAEQMAAAIKSTLPGMDALIMAAAVADFRPQNIASHKLKKGDRNAANLELKRCPDILAEVVPEARPRVVVGFAMETSELLSGASAKLEAKNLDLIVANDLSQAGAGFAVDTNAVTIVTADDVTTAVPLAGRRVFISAGGTREPLDPVRFLGNRSSGKQGAALAAAAHRFGAEVVLAAANVDSAILESLPSGIDIREVETTAQLESVCRAQAAEADIVIMAAAVSDYRPAQAAGHKMKKDGDAGLTIELVQNPDILAGLVADRREGQTIVGFAAETGSPETPADELARQKIVRKGCDILVFNDVSDGKTFGHDTTHVEVLDAAGTVTELPHGSKLALSHAVWDAVASRLPR",
    "response#3": "MNIVLGVTGGIAAYKAAHVVRLLKEAGHSVRVVPTESALCFVGAPTWEALSGQPVQTDVFTRVEEVEHVLIGQQADLVLIAPATADSLARIRAGMADDLLTATVLTTTAPVVVAPAMHTEMWFNPATVDNVAVLRARGVHVLEPASGRLTGADSGPGRLPEPQDIVDFARILLGVCGGVSAFKAILLVRRLQDAGFEVRVVMTDAATRFIGVPTFHAISQNPVHRSVWALDESSAGELHVDLSRWADAIVVYPATANLVGGLSAGLADDLLKLCICCFDGPVLVSPAMHSKMAGHPLHHQALERLNASGITVVPSESGRLASGESGQGRLPEPEVIVAEVERALGLLGPRSDLRGRHVVVTAGGTREPLDPVRFLGNRSSGKQGYALAKAAAARGARTTLIAANVTLPDPAGVELIRVGTTEELRAATVKAAAEADVIVMAAAPADFRPVTVAAEKIKKTDSGTPAPIELTTNPDIAAELGAAKREGQVLVAFAAETHDALENARAKLIKKRADLIVVNEVGVDRVFGADHNEVTLLAADGAEVAVPAGSKTALAHAVWDEVVARLP"
  },
  {
    "reference": "MTVFLPILVCLCGGVGASCRYLLDVTIKTYWQRAFPLSTFTINLIAGFLAGLVAALALGGTLDEPWRLVLATGFLGGFSTFSTAINEMVTLFRKHRYPTAAAYLVLSLGVPVVAAACGFLV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. The designed protein must be optimized for fluoride transmembrane transporter activity. The designed protein sequence is ",
    "response#1": "NVYLLGFTSFLNDMSSEMIMPILPMLITSVGGGSLSIGLVGGLREFISNILMVLIGYCSDKVRKRKIFVVLGYLTSSMFKLLLGLSKSWLGAVIFSSLERMGKGIRTAPRDAIISESMPKTLGKGFGIQRAFDTAGAILGSTLSLLFILYLQYSFNQIILIAAVIGFLTLIPLYFVKEKPSPSNNKITFRVGIKNLPKELKLFILISAIFTLSNFSYMFYILRAQEFLMIVDEKMAIIIPIALYILYNIFYATFSIPFGILSDKIGRKSVLTIGYIVYGIVSLGFAYFISQKSLILLFALYGIAYALFAGNQKAYVSDLSSEDIRATALGLFYTVVGLTSLPASLIAGYLWKISPEMTFLYGSVLAIISGLLLLFAR",
    "response#2": "NVYLLGFTSFLNDMSSEMIMPILPMLITSVGGGSLSIGLVGGLREFISNILMVLIGYCSDKVRKRKIFVVLGYLTSSMFKLLLGLSKSWLGAVIFSSLERMGKGIRTAPRDAIISESMPKTLGKGFGIQRAFDTAGAILGSTLSLLFILYLQYSFNQIILIAAVIGFLTLIPLYFVKEKPSPSNNKITFRVGIKNLPKELKLFILISAIFTLSNFSYMFYILRAQEFLMIVDEKMAIIIPIALYILYNIFYATFSIPFGILSDKIGRKSVLTIGYIVYGIVSLGFAYFISQKSLILLFALYGIAYALFAGNQKAYVSDLSSEDIRATALGLFYTVVGLTSLPASLIAGYLWKISPEMTFLYGSVLAIISGLLLLFAR",
    "response#3": "NVYLLGFTSFLNDMSSEMIMPILPMLITSVGGGSLSIGLVGGLREFISNILMVLIGYCSDKVRKRKIFVVLGYLTSSMFKLLLGLSKSWLGAVIFSSLERMGKGIRTAPRDAIISESMPKTLGKGFGIQRAFDTAGAILGSTLSLLFILYLQYSFNQIILIAAVIGFLTLIPLYFVKEKPSPSNNKITFRVGIKNLPKELKLFILISAIFTLSNFSYMFYILRAQEFLMIVDEKMAIIIPIALYILYNIFYATFSIPFGILSDKIGRKSVLTIGYIVYGIVSLGFAYFISQKSLILLFALYGIAYALFAGNQKAYVSDLSSEDIRATALGLFYTVVGLTSLPASLIAGYLWKISPEMTFLYGSVLAIISGLLLLFAR"
  },
  {
    "reference": "MASTCSRQLLRARYPHHAHPALTRTCVPSRTFSSTRTAWDQQNFHRKESFRSRLNSALKGTKIKWEPIPIALGIGFLGAFQLYRIQRREKHTQAEGERGEEGLDQQGRPKKRDRIRPSGPWTVQVMSTLPLKALSRVWGRFNEIDIPYYLRVPGFKLYSFIFGVNLDEVSEPDLHVYPNLAAFFYRTLKPGVRPLDPNHDAVLSPADGKVIQFGTIEHGEVEQVKGVTYSLDALLGSSRPTSPSQNVADSQIRSSTTHEKVGEEDVVRSDEEFAQVNGISYTLPNLFSGPWPKGGKPAEMPSDQSTAPKASSEAEVRADLAKSEAERPWWSPASTKTQTQLYYCVIYLAPGDYHRFHSPVSWVVESRRHFAGELYSVSPYLQRTMPGLFTLNERVVLLGRWRWGFFSYTPVGATNVGSIKINFDRELRTNSLTTDTAADRAAEEAAARGEPYSGFAEASYGSASRFLGGQPLKRGEEMGGFQLGSTIVMVFEAPKGEGPSPGEGLQGERHGGFKWNIAQGMKVKVGEALGAVQVDESK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have phosphatidylserine decarboxylase activity to facilitate phosphatidylethanolamine biosynthetic process.2. The stability of the protein-pyruvate complex should be optimized to maximize enzymatic activity.3. Prioritize Basic and acidic residues, Polar residues compositional bias within the protein sequence to achieve desired characteristics.4. The Mitochondrial intermembrane topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.5. The Charge relay system; for autoendoproteolytic cleavage activity, Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.6. A protein should be able to manipulate the activity of enzymes in the phosphatidylethanolamine biosynthesis pathway, either by activating or inhibiting their function.7. For general function, the protein need meet that Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.; Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer) The designed protein sequence is ",
    "response#1": "MAVLGYSLLVTTLVFGAVLFSNYFFN",
    "response#2": "MAPDNSTKAASAQSGLRHSSLTTLSPSSPTNASSSSSTTTTTNSSSTSSSRRRSSSSPRRQQQSSSSPTTSSSRSNSSSSTSSSNSSSNSNSNSNSSSNSSSSKTTKSSSSKSSTSSSSSSSSSSSSSSTSATTTSSSSSSSSSNNSSSSNSSSSSKNSSSSKSTSSSSSSKSSSTENSSSSSSSSESSSSNSSSSESSSSNSSSSESSSSNSSSSTSSTNSSSRTTSSSTSSSSSSSSSSSTSSSSKTTSSSSSSSTSSSSSPKSSSSSSSSSSSSSSRPASSSSSSTSNSSNSSTSSTSSTSSSSSSSSSSSSSSSSSSSSSRPASSASSSTSTSSNSSSISSSSGRSSSSSSSTSSNSSSNSSSSSSSTSSSTSSSSSSSSSRPASSSSSSTSNSSNSSTSSTSSTSSTSSSSASSSSRPASSSSSSTSNSSNSSTSSTSSTSSSSASSSSRPASSSSSSTSNSSNSSTSSTSSTSSTSSSSASSSSRPASSSSSSTSNSSNSSTSSTSSTSSTSSSSASSSSRPASSSSSSTSNSSNSSTSSTSSTSSSSASSSSRPASSSSSSTSNSSNSSTSSTSSTSSTSSSSASSSSRPASSSSSSTSNSSNSSTSSTSSTSSTSSTSSSSASSSSRPASSSSSSTSNSSNSSTSSTSSTSSTSSSSASSSSRPASSSSSSTSNSSNSSTSSTSSTSSTSSSSASSSSRP",
    "response#3": "MAPDARRHLVAEDDPWATTDDPDFRGYRRVDRDDPLGPLTRRFRALYPDAPEGTVLYLDGNSLGALPRGVPGRMRELVERDWGGRLIRGWTEAWMGWPERIGDRLAPLVGAAPGQVVVTDSTTVNLYKLAVAALRMRPGRTVLLTEPGNFPTDLYMAQGLADDLGLGLRLTPVDEADLAAALDDEVAVVVLSHVDYRTGRLHDLPGITRAAHAAGALVLWDLCHSAGAVPVELDAAGADFAVGCTYKYLNGGPGSPAYLFVAARHQEAARSPLPGWNSHADPFGMRAEYGPAPGVERMLTGTPPVLSLLALEAALDVWDGVDVEAVRAKSLALGDLFIELVEARCPGFTLASPRDPERRGSHVTLAHDEGYAVVQALIARGIVGDFRPPDVLRFGFTPLYTSFADAERAARVLGELLADGEWRAERFAGRAAVT"
  },
  {
    "reference": "MRIVFMGTGDIAIPAFRSLIRHSDLAGLVTQPDRPVGRHQVLTAPAIKNIAREAGIPVLQPHSLRSPDALSNLRRLNPDLIVVMAYGQILSQEVIDMAPMGCINAHASLLPRHRGAACIQSAIKSGDAETGITIMHIVRKLDAGDIIAQISTPLEGSETGGTLHDKLARMTPDVLLPVIHSIEKGTATRIRQQEILATYAPKLLRADGKIDWTRPAEEIGRMIRAYDPWPGTFTNYWNRKKRIRNMKIFPGFSILPEAEGKPGQVLSAGEQGLLIACGSGGLLVTDVQLEGSTRMNISQLIAGHPNLKDIHFDV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be able to perform methionyl-tRNA formyltransferase activity under specific conditions. The designed protein sequence is ",
    "response#1": "KISAGTSCIKWEEQTSEQIFRLYRAIGNIIPLQTLWMANTIKLLDLVEVNSSVLADPKLTGQALIPGSVIYHKQSQILLVYCKDGWIGVRSVMLKKSLTATDFYNGC",
    "response#2": "KISAGTSCIKWEEQTSEQIFRLYRAIGNIIPLQTLWMANTIKLLDLVEVNSSVLADPKLTGQALIPGSVIYHKQSQILLVYCKDGWIGVRSVMLKKSLTATDFYNGC",
    "response#3": "DGERLTGCVSTQVGCRMGCSFCSTAKIGLIRNLKIGEIVRQIIIMNRLLSEKNEKLSNIVFMGMGEPLDNLDNLNEAIKIILDENGLNFSHRKVTVSTCGMVNELKKMYEELDSPVNLAVSLNFTDNKIRQRYMPVSKKYPVEALISTLRNLPLQKRKRITIEYVLLKNINDSQMDAKYLAEVLRGLPVKINLIVYNDSDIGFNSAADENVLAFQKVLLDKGLSAFIRKSLGSE"
  },
  {
    "reference": "MSFRLDAASVSYANGQRALSQITLAASRGEPIAVIGPSGAGKTSLLRLLATSLRPSEGRVEVLEQSPWAVSAARLQQLRCRIGMVHQAPPMPPRQRVVTAILAGRLGIWPAWKSLLSLVYPADIDGAGEQLARLELADRLFDRCDQLSGGQLQRVGVARVLYQRPELILADEPVSAMDPVLANLTLGELRREADTRAVPLVASLHAVDLALRWFPRIVGLKAGEIAFDLPAERVTDALLRELYASESASPPVQGSQALTSIAAGASAAIGGDTQPRPACR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. We need a protein that can perform ATP + H2O + phosphonate(out) = ADP + H(+) + phosphate +   phosphonate(in) with high efficiency.2. The protein contains novel ABC transporter domains that confer a unique function or activity.3. The ATP binding site should be located in a region of the protein that is accessible to the ligand.4. The protein should be optimized for plasma membrane-specific folding and stability, while maintaining ATP hydrolysis activity, ATP binding.5. For general function, the protein need meet that Part of the ABC transporter complex PhnCDE involved in phosphonates import; Responsible for energy coupling to the transport system. The designed protein sequence is ",
    "response#1": "METKNLNLWYGEKQALFDINLPIYENKITALIGPSGCGKSTFLRCLNRLNDLIPNVRIEGEVLLDGKNIYDKDVDVYELRKRVGMVFQKPNPFAMSIYDNVAFGPRIHGIKDKKELDKIVEWALKKAALWDEVKDELHKNALSLSGGQQQRLCIARAIAVKPEVLLMDEPTSALDPISTLKIEELMVELAKDYTIVVVTHNMQQASRVSDYTAFFLMGKLIEFGETEQIFLNPQKKETDDYL",
    "response#2": "MRAVDLTLGFGPKTVLEQVSLDFPARSVTTLLGPTGSGKTTFLRTLNRMNDKVSGFRHSGDVLLGGRTIFADRDLMEFRRSVGMLFQRPNPFPMSIMDNVVAGVRAHKMAPRKQFKTVAEARLTEVGLWDAVKDRLGDSPFRLSGGQQQLLCLARALAVNPDVLLLDEPTSSLDPTTTEKIEGLIRSLADRLTVIMVTHDLAQAARTGDRTAFFFEGRLVEEGPTKHLFLSPQHEETVRYR",
    "response#3": "MRAVDLTLGFGPKTVLEQVSLDFPARSVTTLLGPTGSGKTTFLRTLNRMNDKVSGFRHSGDVLLGGRTIFADRDLMEFRRSVGMLFQRPNPFPMSIMDNVVAGVRAHKMAPRKQFKTVAEARLTEVGLWDAVKDRLGDSPFRLSGGQQQLLCLARALAVNPDVLLLDEPTSSLDPTTTEKIEGLIRSLADRLTVIMVTHDLAQAARTGDRTAFFFEGRLVEEGPTKHLFLSPQHEETVRYR"
  },
  {
    "reference": "MLEIEKPKIECVARSDDATYGKFVVEPLERGYGVTLGNSLRRILLTSLFGAAVTSVKIEGVLHEFSTIPGVVEDTTDIILNLKQLAIKMHTEESRTIRVEFEGEGVVTAADILTDADVEILNPDQVIATVDKGRLFMEITVEKSRGYITATKHRKSEHAIGVIPIDANFSPVRKVNYRVEDTRVGQITNYDRLILEVWTNGSLTPEEAISWSAKIMNDYLKLFVALTENTENVEIMVETEERPQDKILEMTIEELDLSVRSYNCLKRAGINTVKDLTNKTEEDMIKVRNLGRKSLEEVDAKLAALGLSLRKSED",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a fast turnover rate for a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +   RNA(n+1).2. The protein can be expressed in DNA-directed RNA polymerase complex and perform DNA binding without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "KIKKVKHEYSTIIGLQESIHDILINLKEIVLKSESFEPQKAYISVLGPKKITAQDIKGPSCIKIMIIAQYIATLNKDILLEIELNIEKDRGYRIENLQKYQEGLFPVDAVFG",
    "response#2": "LIEVQRESYEQFLRSNKEIDYVSGLEKTLRSVFPIRDFAGTAELDFVHYELEPPKYDTTECRQRGITYAAPMKVTLRLIVFEVDQETETRSVLDIKEQDVYMGDMPLMTGNGTFIINGTERVIVSQMHRSPGVLFDHDRGKTHSSGKLLFAARVIPYRGSWLDFEFDAKDIVNVRIDRKRKLPVTALLYALGLDSEEILHFFYNTVTWKRAGGKKGEGWKIPFEPEAWRGQKPTFALVDAKTGEEVFPANQKISPRAANKAQKDGLKDLLLPTEEVFARYAAKDMIDEKTGRIYIEAGDEVGPDHLEALDAAGIDELELLDIDEINTGPWIRNTLKVDKAENRDEGLEAIYKVMRPGEPPTKETAEALFEGLFFDGERYDLSAVGRVKLNMRLDLDAEDTVTTLRKEDILAVVKELVGLKDGKGDVDDIDNLGNRRVRSVGELLENQYRVGLLRMERAVKERMSSVDVSTVMPNDLINAKL",
    "response#3": "RSPGIYYKSEADKQGVRIYTASLISNRGTWVKFEIDKDDLVYIKIDKAKKFSIFVFLKALGLDNQEIFNSIENINYFRKTSNTDEQLTTEQCLLEVHSHLKPEEPATAKGCQKLLYAKFFDSKKYDLGYVGRHKLNQALMLDIDSDVRILTSLDLIGIINQLIRFRFMPIVSDDIDHLGNRRIYIGQFVAQPLERGYGVTLGNAMRRVLLASLPGTAITGIKIDGVFHEFSTIDGVREDVPEIVLNLKKVRFKSTTKRSCKTSLSIEGPADFKAGDIVAQEGEFEVLNKDMHIATLNGDAKLNIDIYIGRGRGYVPAEENRGEGMPIGFIAIDSIFTPIKNVKFSVENTRVGQRTDYEKMILDVETDGSISPDDSISLAGKVINEHVSLFANFIRNTLAVDKCTNREQALIDIYRVMRPGEPPTADTAEALFKSLFFDAERYDLSAVGRVKMNMRLDLDVSDTVRVLRKEDILAVIKTLVGLRDGKGEIDDIDNLGNRRVY"
  },
  {
    "reference": "MVRDKWKDKVWYTILAPDMFDNVEVGETPADDPEKVIGRVLETTLGDVLDDITKHHIKVFFRIYDVEGTTAYSKFEGHRLMRDYVRSLVRRGTSRIDGVIDVVTKDGYKVRVAGLAFTTRRAKTSQQRAIRKEMFKVIEENAKECDFDEFIRRCLSISEEESIPEQIKEAGRKIYPIRQAEIRKTEVLEEPNGLPPYEAVGDRATPELASY",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be involved in translation by localizing to ribosome, ribonucleoprotein complex and exhibiting structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "SLKLQKRLAASVLRCGKKKVWLDPNEINEIANTNSRQNIRKLIKDGLIIKKPVVVHSRYRVRKNTEARRKGRHCGFGKRKGTANARMPTKLLWMQRQRVLRRLLKKYRDSKKIDRHLYHDLYMKCKGNVFKNKRVLMEYIHKKR",
    "response#2": "DIERGIRVAVDTGKVILGSNKAIQAIKLGNGELAIMAENTPKNVKADIEAYSKLSEMPVYTFEGSSVELGSICGKPFTVSVLIIQEPGDSNIL",
    "response#3": "IPPTTALVMKEAGVEKGSGTPNTQAVGNLPLEAVIRIAKMKMESMLSYDLKTAAKEVMGTCVSVGVTL"
  },
  {
    "reference": "MNPETLKFIEASIKTIPDYPKPGILFRDITSLIENAEAFKATIDLLAEHYRDQGITKIVGTEARGFIFGAPVAFAMGLGFVPVRKPGKLPRAVIEESYALEYGTDTLQLHTDAIVPGDKVLVVDDLLATGGTVDATVKLIRRAGGEVADAAFIISLPSLGGDARLTAAGIKVVSLVELAGE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Ideal protein should have a binding affinity for key enzymes in the AMP biosynthesis via salvage pathway pathway to enhance the overall efficiency of the pathway.2. The protein must exhibit the following characteristics: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis..3. The designed protein should be involved in AMP salvage by localizing to cytoplasm and exhibiting adenine phosphoribosyltransferase activity. The designed protein sequence is ",
    "response#1": "MIDMIANRYIGQRIDKVVGVEARGFLIGAALAYRLGAGVVLVRKPGKLPSETFSKTYDLEYGTDTLEIHKDAIKPGEKILIADDLLATGGTMAAVVDMVTNMQGEIVECCFMTELTFLDGRKKLPEGKVYSRG",
    "response#2": "MDALVQLCLEANLKPDHVVGMESRGFIFGPTLAYNLNAGFVPVRKPGKLLAAVHTVEYELEYGTDTLEIHQDAVGSGDKIVIVDDLIATGGTAKATAELLTKIGCDIIGFVFIVELLDLKGRDRLPDAPVLSLS",
    "response#3": "MDALVQLCLEANLKPDHVVGMESRGFIFGPTLAYNLNAGFVPVRKPGKLLAAVHTVEYELEYGTDTLEIHQDAVGSGDKIVIVDDLIATGGTAKATAELLTKIGCDIIGFVFIVELLDLKGRDRLPDAPVLSLI"
  },
  {
    "reference": "MAGMITFGSFAPVPIAGCTHPEMLKVAKPRVAKQVLTTRLGTNLSANRSAGRALIKAYDEQQARFEEQLAHMGVRSVQQKTPKVCKTFDGFDSVVTHIVIPSTTRVENDKSLQVKHPAVVYRKRKTPPRQNYVTLDERGLSDLFTELSQYILLDRQVMQILQAGKRKPITISRCNVGPYTTLHVTTKHELGKMAPIDLSLDEDSESILQSFANTLPTTLDIREEDFRPGTSGAIIRRDCLDNVAHKVPDSDYFIVRGRFAGSLLDARETFNISHRFYLRQYSVADQFWKGFDTEFRQQRGRRTIHEGMNTLSVEATGSVAAIACQALYPCCRITCTECGRIYDRMSETQLSDHVQAGLRAGIETLRNRFPEFHHVQDILERYSTSFNNSGNNSEAHGRIKFSIGDINEPPFTYIHQISNTIFKGGRASKVEFETAMNLLLETTRWLRNRHDRIAKDPLSAFRNKVSGKALLNPTLMCDNQRDVNGNFIWGKRGYHAKRFFSNYFSVIEPQHGYDAYVTRTFPNGTRKLAIGSLILSTNLDELKAQLEGESIHKEPLSIKCTSEKNNSYVYPCCCVTNDVGVPIYSGLKTPTRNHIVVGNTGEAKYLDLPVEISEKLYIAKEGYCYINIFLAMLVNVDEKDAKTFTKWVRDVIATQLKTWPTMHDVALACYQLSILFPSVRSAELPRILVDHHTTTMHVIDSYGSYTTGYHILKANTVEQLIEFASDALKSEMKHYRVGGDMTNNLMLHSSLRTLIKSIYRPELMHSIL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The ideal protein would be one that plays a critical role in proteolysis, viral RNA genome replication, RNA-protein covalent cross-linking, DNA-templated transcription through cysteine-type endopeptidase activity, helicase activity within host cell cytoplasmic vesicle, helical viral capsid.2. The protein must exhibit the following characteristics: Involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification..3. The designed protein should contain one or more Peptidase S30, Helicase ATP-binding, Peptidase C6, Helicase C-terminal, Peptidase C4 domains that are essential for its function.4. The protein with the reaction Hydrolyzes glutaminyl bonds, and activity is further   restricted by preferences for the amino acids in P6 - P1' that vary   with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or   Gly) for the enzyme from tobacco etch virus. The natural substrate is   the viral polyprotein, but other proteins and oligopeptides   containing the appropriate consensus sequence are also cleaved., the For helper component proteinase activity must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "RMVKSSHNLWLLTFLKWSAIYCALFNIPTLGWNILERRDNASMRNMFRQIMGVGTQRLAAPLKAWKTSKSWIKPEIWASTIQRLASAVRHVQKIRDAEVSQGWSGIVMDQRQPLIHKISRSSIFVVRGRLNGKLVSACDTFCWNSVLKVVQKHFKATVHANVESIMKEMFQTVSENVLIEVIWNKGRKVLGRKFKTPEQQYFKIETKHEHGIIEEVDCPITESQVKTLSYITSIFTKGKSIHEKKIKHGWSGCVIPKYALIGDHFSTSNSFIIVRGRSQTLLIDSAARTPIELLPTVEHKHFKATVHANVESIMKEMFQTVSENVLIEVIWNKGRKVLGRKFKTPEQQYFKIETKHEHGIIEEVDCPITESQVKTLSYITSIFTKGKSIHEKKIKHGWSGCVIPKYALIGDHFSTSNSFIIVRGRSQTLLIDSAARTPIELLPTVEHL",
    "response#2": "VMTDSQFNWFVKQSKNIMTEKRGCIELVGQKTTKLYYKSTRFGVRCTCELAHVRGERKRVDAVIPRDHVPLAKVLARVPTWGRAIHSEELRRGDSGMILRSESCKGTFGRSWDEMFIVRGAYEGKILDARSKLTETTVLRMVQCLSTQSVDHLVKALFKVTKHKEMEFQVIGKKKSHNRVFKFKRFEHRMHTFVHLKHHDGKMHRVDCKLGAYATHILQLMACATSKTHSIKVDNLQPGSSGLVLNHKILRGKVSRQRDGMFIVRGRCDGELLEARSKFSHSVVHRMTHSLTDTQLLNLVKQVKNIGADKNLTIEVIGKRRNVIRYKDFRTNVGAFIEVQHVLGKRARVDLRIPNSNIPFVQVLAKTNAWGMKVKTLDLKRGDSGVIRNPKKLYGKQGRSTHDMFIVRGNCEGQVFDARSRVTFSIMSRMTHCLSTQSVDHLVKALFKVTKHKEMEFQVIGKKKSHNRVFKFKRFEHRMHTFVHLKHHDGKMHRVDCKLGAYATHILQLMACATSKTHSIKVDNLQPGSSGLVLNHKILRGKVSRQRDGMFIVRGRCDGELLEARSKFSHSVVHRMTHSNQN",
    "response#3": "ELSSTQLQSLQRTLIKLARDAKKPVEIIDRHSVKMQFYDRQQLTYARVEVRHLRGKLRRVDVHSTPFINKFLNSVVKLTAGNRKHASDDITFGWSGTVLNRQKIQGGLTPTNAQSFIVRGKDGKRLIDARTRISIGRMKRMKHITKHTTPDPSLVRLQSRQRTTHASDKHNENNISELIHKLTRIVHDRQLDVHFVGKRRLDGRCVRSDGKSYLQLQTKHTLGVQSGVDCYIPKGLEHLVHAFAKGSGYKSRIQVSKIKKGWSGFILQPTKIIGQVGKCKHDFLVVRGSLRGELIDACTRKHFKATVHANVESIMKEMFQTVSENVLIEVIWNKGRKVLGRKFKTPEQQYFKIETKHEHGIIEEVDCPITESQVKTLSYITSIFTKGKSIHEKKIKHGWSGCVIPKYALIGDHFSTSNSFIIVRGRSQTLLIDSAARTPIELLPTVEHHFVHSQDSDCLSTQSVDHLVKALFKVTKHKEMEFQVIGKKKSHNRVFKFKRFEHRMHTFVHLKHHDGKMHRVDCKLGAYATHILQLMACATSKTHSIKVDNLQPGSSGLVLNHKILRGKVSRQRDGMFIVRGRCDGELLEARSKFSHSVVHRMTHCLSTQSVDHLVKALFKVTKHKEMEFQVIGKKKSHNRVFKFKRFEHRMHTFVHLKHHDGKMHRVDCKLGAYATHILQLMACATSKTHSIKVDNLQPGSSGLVLNHKILRGKVSRQRDGMFIVRGRCDGELLEARSKFSHSVVHRMTHD"
  },
  {
    "reference": "MGAIPQISLIGLSGVGKSTIGEMIAANLGWSFLDTDILIEEETGSNPAELITSKGELFFREIEERVIIEATQNEFVVISTGGGAFLSPQNRRNLGANGYICFLDATPKVISEHLKNYPRIVPRPLLGDSPEALESRLYQLDKERRDTXQHADLWVPVQNMFNDFENRSDAISEVVTRILRAWSSESRQLLESSRRLERLAEIDLPAPPSAIVDTGIDKYPIWIGPEELNFLPDRLRQLDLTNRRVFMICDSNVIDLHGTSIAEILDRDGIAGASYILPAGEESKKLEIAKEIYTWLGTQNAERRDLILAVGGGVVGDLAGYVASTYLRGIPFIQIPTTVLAMNDAAIGGKVAVDLPDGKNLVGSFYQPKAVISDVSTLTTLPSRSFNEGFAELIKHGFILDPDLLFEMKNLGNVHTLQHDVTRLSELTARSARLKALVVSSDPREENLRMILNYGHTIGHAIETVSNYTEYLHGEAVAIGMMGAARIARHLEMIDDELLAMHSDLLRLFSLPTEMSDMNINSILQAMKRDKKVESGKLRFVLLEDIAKPVVRNDVPESVVIRVLKEISRS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should contain one or more 3-dehydroquinate synthase domains that are essential for its function.2. A protein that is capable of catalyzing reactions within the chorismate biosynthesis, Metabolic intermediate biosynthesis, chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7 pathway with high efficiency and specificity.3. NAD(+) is required for the proper folding and stability of the protein.4. A protein that can perform ATP binding, 3-dehydroquinate synthase activity, shikimate kinase activity, magnesium ion binding in cytoplasm to support chorismate biosynthetic process, phosphorylation, aromatic amino acid family biosynthetic process, amino acid biosynthetic process.5. For general function, the protein need meet that Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.6. The protein have enhanced ATP + shikimate = 3-phosphoshikimate + ADP + H(+).7. The NAD(+), Mg(2+), ATP, Zn(2+), substrate binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MLVPDAEAGKTIQVASGLWARLGDAGFTRSDAIVGLGGGAATDVAGFVAATWMRGIRYVNCPTSLLAMVDASTGGKTGINTDAGKNLVGSFYTPAGVLADTSTLSTLPNDIMVARDKITGYAEIVKSGLIGDEALFTWCEKHGAAVLSQEPGALAEAVEKACAFKAAVVTKDEFERSSEGGRALLNLGHTFGHALEAELGYDGRLLHGEAVSIGLRLAFLLSVRLGYCSGADLDRVTRHLESNGMPVRISDIGEHFSAETLLHHMTRDKKAKEMGIEGILVNGMAEMLKHGVIGDAALFSALATGRYPSSQEIERAVRVKVSVVAKDPSETGRRMVLNLGHTVGHALERLSRYSLRHGEAVGIGMLAAARIAAAVGLADSSLSGQIEAALDACGLPYRCPPFPVEAIWEAMTHDKGYAEIVKSGLIGDEALFTWCEKHGAAVLSQEPGALAEAVEKACAFKAAVVTKDEFERSSEGGRALLNLGHTFGHALEAELGYDGRLLHGEAVSIGLRLAFLLSVRLGYCSGADLDRVTRHLESNGMPVRISDIGEHFSAETLLHHMTRDKKAGEVYEKIGLGEVAKSGFILDGEILKILSDSNNAKLLREFDGDQLENNNELHELLVELIERTVRVKAYHVSSDLKEQGLREFLNYGHTLGHAIEQIEHFRWRHGNAVAVGMVFAAELAQILGYLDTQDVSYHRSLLDSLGLPTSWQSREDADSAFERVLNLMHRDKKVYRLEEK",
    "response#2": "MLVPDAEAGKTIQVASGLWARLGDAGFTRSDAIVGLGGGAATDVAGFVAATWMRGIRYVNCPTSLLAMVDASTGGKTGINTDAGKNLVGSFYTPAGVLADTSTLSTLPNDVRIAQDRIAGYAEIVKSGLIGDEALFTWCEKHGAAVLSQEPGALAEAVEKACAFKAAVVTKDEFERSSEGGRALLNLGHTFGHALEAELGYDGRLLHGEAVSIGLRLAFLLSVRLGYCSGADLDRVTRHLESNGMPVRISDIGEHFSAETLLHHMTRDKKVKHTLNWFTGLGEVVKCGFIADPAILDLVEGVAPEEITADSPVLRELVERAIGVKIAVVVTDLKETGGVDGHPGREALNYGHTLGHAIERAEGYRIRHGEAVALGCVFVAELARRTGVLSADVAARHRSVLARVGLPTTYAGAGFEELLAAMRVDKKG",
    "response#3": "MLVPDAEAGKTIQVASGLWARLGDAGFTRSDAIVGLGGGAATDVAGFVAATWMRGIRYVNCPTSLLAMVDASTGGKTGINTDAGKNLVGSFYTPAGVLADTSTLSTLPNDLALADERKIVGEAKNYAGYAEIVKAGLIADPALYEWCEAHGAALLGGDQGLQEEAIARAVAFKAQVVMADPREQAKRDGRALLNLGHTFAHAFEAETGYGEALLHGEAVAIGMVCAFDLSARLGYCDAGIVPRIAAHLDEVGLPHRIGGWNAEALLGHMQSDKKVATLIDLHD"
  },
  {
    "reference": "MAQYSAQFPHAKVLVLGDVMLDRYWFGATNRISPEAPVPVVRVKKHEERAGGAANVAMNIASLNVPVRLLGLTGDDEAGNALTGLLEKQKICCDFVKLSSHPTITKLRILSRHQQLLRLDFEENFNNVHSDNLLTKLESAVKNVGALILSDYGKGTLNDVQHMIRIARQAKVPVLIDPKGTDFERYRGATLLTPNMSEFEAVAGVCQSDEDIVEKGLKMIADYDLTALLITRSEKGMTLLRPNQPAFHLPTEAKEVYDVTGAGDTVISVLATALADGRTVEEACYLANVAAGIVVGKLGTSAVSTVELENAIHGRTVSGFGIMTENELKNAVKLAKERGEKIVMTNGCFDILHPGHVSYLENARKLGDRLIVAVNTDESVKRLKGETRPINDLASRMAVLAGLSSVDWLVAFDEDTPQRLIGEILPDLLVKGGDYKPEEIAGSKEVWANGGDVSVLNFENGCSTSNVIKKIRDLKD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must have high specificity towards heptose-1-phosphate adenylyltransferase activity, phosphotransferase activity, alcohol group as acceptor, ATP binding, heptose 7-phosphate kinase activity.2. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.3. We require a protein that can specifically bind to the metabolites produced in the ADP-L-glycero-beta-D-manno-heptose from D- glycero-beta-D-manno-heptose 7-phosphate: step 3/4, Nucleotide-sugar biosynthesis pathway.4. For general function, the protein need meet that Catalyzes the ADP transfer from ATP to D-glycero-beta-D- manno-heptose 1-phosphate, yielding ADP-D-glycero-beta-D-manno-heptose.5. The protein with the reaction ATP + D-glycero-beta-D-manno-heptose 1-phosphate + H(+) = ADP-   D-glycero-beta-D-manno-heptose + diphosphate, the active site must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MRVKGLRALVIGDLMLDEYLWGKAERISPEAPVQVVDVRREDMRLGGAGNVVNNLVAFGACVSICSVIGGDENGTVLRHAFTGKGVDVSGVFEDPMRMTSKKTRVLAANQQIVRIDRETREEISPEFEEMILAHVRDTRNIWDLFLISDYGKGLLTSRVLSGVIASAQERGIPVIVDPKGSDYRKYRGATIITPNRKEAEVASHIAITDAESLNRAADFLLAEGEYQALLITRSSEGMSLFLNAGEEVHIPTVAREVYDVTGAGDTVVATFGVGLACGLSFERAAQLANVAAGIVVGKVGTSTVSPEEIIGLLGVEDEAGSTSSSTREARGRGERVVMTNGCFDILHQGHVAYLEEAKALGDRLLVAVNDDASVTKLKGAGRPINPVSDRMSVLAGLASVDWVVPFSEDTPADLIGEVLPDVLVKGGDYQPEEIAGADAVLSNGGTVKVLSFSEGRSTSSIIDAITS",
    "response#2": "MRVKGLRALVIGDLMLDEYLWGKAERISPEAPVQVVDVRREDMRLGGAGNVVNNLVAFGACVSICSVIGGDENGTVLRHAFTGKGVDVSGVFEDPMRMTSKKTRVLAANQQIVRIDRETREEISPEFEEMILAHVRDTRNIWDLFLISDYGKGLLTSRVLSGVIASAQERGIPVIVDPKGSDYRKYRGATIITPNRKEAEVASHIAITDAESLNRAADFLLAEGEYQALLITRSSEGMSLFLNAGEEVHIPTVAREVYDVTGAGDTVVATFGVGLACGLSFERAAQLANVAAGIVVGKVGTSTVSPEEIIGAVLAPAKLPVAEQRILVIGDLMLDHWVWGSVSRISPEAPIPVVDVQRHSYTPGGAANVVSNLKALGSQVEILGVVGADDPGRRLRALLRRQGIGTEGIFIQKARPTSLKTRIIAHSQQVVRADLEQRKAVDEALWTQILAWLDENRERFTGFLLSDYNKGLFWDGRLREMLRCLEGRPVLAGPKPENIEEFRGVEMLALNALEARVASGHDTTSEEGLQRAGEDLLERLGCRAVLVTLGERGMALFEEGRPWKQVPALASQVYDVSGAGDTVLSVMGLARSVGVAPEEAMRLASHAAAVVVRKVGTAERAHDDRHTERMMAAEQLAMAREHSRGKKVVFTNGCFDLLHVGHVKYLQKARGLGDILVVGLNTDASVRRLKGEKRPLIEETERAHILAALDCIDYVVLFDEDTPLKVIETLGPDILVKGGDYSIEGVVGREVVEARGGRVELIQFVDGRSTSRIIEKIRG",
    "response#3": "MRVKGLRALVIGDLMLDEYLWGKAERISPEAPVQVVDVRREDMRLGGAGNVVNNLVAFGACVSICSVIGGDENGTVLRHAFTGKGVDVSGVFEDPMRMTSKKTRVLAANQQIVRIDRETREEISPEFEEMILAHVRDTRNIWDLFLISDYGKGLLTSRVLSGVIASAQERGIPVIVDPKGSDYRKYRGATIITPNRKEAEVASHIAITDAESLNRAADFLLAEGEYQALLITRSSEGMSLFLNAGEEVHIPTVAREVYDVTGAGDTVVATFGVGLACGLSFERAAQLANVAAGIVVGKVGTSTVSPEEIIGALSRGRGRDTRILVIGDLMLDHWVWGSVSRISPEAPIPVVDVQRHSYTPGGAANVVSNLKALGSQVEILGVVGADDPGRRLRALLRRQGIGTEGIFIQKARPTSLKTRIIAHSQQVVRADLEQRKAVDEALWTQILAWLDENRERFTGFLLSDYNKGLFWDGRLREMLRCLEGRPVLAGPKPENIEEFRGVEMLALNALEARVASGHDTTSEEGLQRAGEDLLERLGCRAVLVTLGERGMALFEEGRPWKQVPALASQVYDVSGAGDTVLSVMGLARSVGVAPEEAMRLASHAAAVVVRKVGTATVDREELLRARKGRRRRPTERATSIRALVVGDLMLDEYLWGRAERISPEAPVQVVEVAREDLRLGGAGNVVNNLAALGCQVSVCSVIGSDENGALLRRALEEKGAGLEGLFEEGERRTSKKTRVLAANQQIVRIDRETKSSIAAASEQGIIDYLAARSGDFDVIVVSDYLKGVLTPAVLSAVCRSGRELGIPVVVDPKGNDYGKYRGATILTPNRKEAEIASGVAITDQESLQCAASGLLSCLELDALLITRSEAGMSLFPAAGGAVHIPTVAREVFDVTGAGDTVISVLSLGLACGLTMADAAWIANVAAGIAVGKLGTSTVAPQEIV"
  },
  {
    "reference": "MDLVLATRNQGKVEEMRPMLASVGFNLINQTDTDIPSAVEDGATFVENALIKARSVAKATGLPSIADDSGLVVQALHGAPGIYSARYAGEDASDADNNQRLLEALSEQSDRRAFFFCCMVFLRSAEDPTPLIATAQWHGEILQAPRGDGGFGYDPLFWLPEHKQSAAQLPKATKNSISHRGQACAKLVEALNVHANN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The protein design must be able to perform metal ion binding, ITP diphosphatase activity in order to contribute to nucleotide metabolic process.3. The protein should be able to bind Mg(2+), substrate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MVIDDNKR",
    "response#2": "MRIKV",
    "response#3": "GCCSCCAG"
  },
  {
    "reference": "MRAAWSVDAVRAAEKATGDDFTNGALMQRAARGLADIVSREVVELERRGVTSPRVVVLVGPGNNGGDALYAARDLAVDGIECLALLVADKAHVDGVAAARAAGVDVARYESASHSGAEPEGGGRENAHALAHADLVVDGILGIGADPGRASTWQELADLIPIQAFVVAVDCPTPGFRADITVTFGGPKTSLMLAPAANGRIEVADIGVHLPLGEREAMRLSGTDLAHLWPVPGPRDHKYTRGVVGLATGSDAFPGAAVLGAVAAVTAGAGMVRYVGPRRAQDAVTAAAPEVVHGSGRVQAWVVGSGIDGPSEREHESDRYDSAMAALRSEEPVVIDAGALTWFEPGVRPEGAQTVIAPHAGELATLLQSRDIDVERSTVEADPVTWARRAANETGATVLLKGGVTVIASPGEGPVTIENRAPHWLATAGTGDVLAALLGVLLAAGLEPRSAAALAAYTHGRAAHLANPDGPVRALDVATSLGRVVSELVAEQRRVHEERRAGRFPR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP; Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration..2. The designed protein should have a binding site that is specific to the target (6S)-NADPHX ligand.3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.4. The protein must have high specificity towards NADPHX epimerase activity, metal ion binding, ATP binding, NADHX epimerase activity. The designed protein sequence is ",
    "response#1": "MRQVEARIFAAGMPVAALMEKVAIAIAQRIAELYPAPMRVGVLVGSGHNGGDGLVVARELHFRGYPVSIYCPSTQLKELTGYHANYCQSLGIPFVDQVAVLQDCDLLVDALFGIGLDRELSTDLVAIINRVNTWMKPVVSIDLPSGLHTDTGQALGSAIQATHTLCLGLWKQGLLQDQALKFVGKAELIDFDVPLADVEDADVAAAWPVPGPQDHKYTRGVVGVVAGTTTYPGAAVLTAAAAVRAGAGMVRYQGPDDVARAVVAARPEVVPADGRVQAWVLGPGLPADGVDDGQRGRARAALAAASGLLAGTQDPVPAVVDAGALALLDRPCPAWVVLTPHAGELAALLVAHGEDVERVGVEAEPLRWARRAHELTGATVLLKGGATVVVGPGVTYAQADAPGWLATAGAGDVLAGLLGAMLAARSAEVVAHPERAAAVAAAAALVHGRAAERATPGGPVAALAVADAIPGVVAGEDADVAAAWPVPGPQDHKYTRGVVGVVAGTTTYPGAAVLTAAAAVRAGAGMVRYQGPDDVARAVVAARPEVVPADGRVQAWVLGPGLPADGVDDGQRGRARAALAAASGLLAGTQDPVPAVVDAGALALLDRPCPAWVVLTPHAGELAALLVAHGEDVERVGVEAEPLRWARRAHELTGATVLLKGGATVVVGPGVTYAQADAPGWLATAGAGDVLAGLLGAMLAARSAEVVAHPERAAAVAAAAALVHGRAAERATPGGPVAALAVADAIPGVVAGTLAADEAWPAALIAAAEAPLTAGTDRYMRAAAHAVAAAALHELGEHRGGAAGARVLALAGGGHNGADTLLAAAALARRGCAVSAALATDHPLPGALHRARAAGVRIADSPREAVGGFLEAGGDLVLDGLTGIGASGGLRPRAAALIAPLLDRTAGSAAGARAGGRPFRVLAVDLPSGTGVDDGTLPGPVLPADRTVTFTCLKAALCLPPACRLAGRVEVVDLGLPVPQG",
    "response#2": "MRQVEARIFAAGMPVAALMEKVAIAIAQRIAELYPAPMRVGVLVGSGHNGGDGLVVARELHFRGYPVSIYCPSTQLKELTGYHANYCQSLGIPFVDQVAVLQDCDLLVDALFGIGLDRELSTDLVAIINRVNTWMKPVVSIDLPSGLHTDTGQALGSAIQATHTLCLGLWKQGLLQDQALKFVGKAELIDFDVPLADVDDKYTQGVTGIAAGSATYPGAAVLATGAALLSTSGLVRYAGPAAEDVRARWPESICTGSITDAGRVQAWVVGPGLGTGLGAEAVLRTVLEAGVPVCADADAITMLANNPDLWDARDPDTLLVLTPHDREYERLAGPVGTDRVTAARKAAERYNAVVLLKGHATVIAGPDGRVLVNRARSSWAATAGSGDVLSGVLGSLLAAGLDPVLAAGCAAKVHALAADLASDGAPIPATSLLAALPAA",
    "response#3": "MRKADASVIRSGTPGVTLMQNAGAAVADAAREMVSPGARIVVLAGPGQNGGDGIVAATRLREQGYRVSLGLLGKREAFSGDAALVAREWPSDVVAVDGANLAPAELVIDALFGGGLSREIEGAARAAIDRINMSGGPVLAVDLPSGIDGETGAVRGIAVRAARTVTFAARKPGHYLLPGRTFCGDVTVADIGVGSSDLPSSHKYERGHVLVASGGATRTGAARLAARAALRIGAGVVTVASPPEALGVNAAQLTAIMLRGCDGPEGLHAILADERLNALVVGPALGVTAVTRAMVAVAAQARRRLVLDADALTSFEGLASELHQGFRSAPTVITPHDGEFTRLFKGHPDILDPSSKVERARRAAAYLSAVAVLKGADTVIAAPDGRAAINANGTPYLGTAGSGDVLCGIIAGLMAQHLPAFEAAAAAVWIEPADYADVVAPHTNNVDKYSRGSVLVVAGSSRYPGAAVMAAKAAARAGAGYVTLAVPVTIAPLIQMQLPEVPVVGVAAEQDGTFSAAARPVILKLAERSSATLVGPGMRVTGGTVAVVSALINSKAPLVVDADGLNCLARLTNGHLDEFPEVTRRDAPLVLTPHRRELGRLVGLADTPPDSLTAALEAARRIVWADGGSELCIVAKGTATACVGVEAALLPEPGTASLATAGSGDVLGGIICALLARMPDDIEDLPLIAAFGCEVHAVAGSRAAKRYGSRGVMAGDIADEVGLAVDEL"
  },
  {
    "reference": "MEAVPRMPMIWLDLKEAGDFHFQPAVKKFVLKNYGENPEAYNEELKKLELLRQNAVRVPRDFEGCSVLRKYLGQLHYLQSRVPMGSGQEAAVPVTWTEIFSGKSVAHEDIKYEQACILYNLGALHSMLGAMDKRVSEEGMKVSCTHFQCAAGAFAYLREHFPQAYSVDMSRQILTLNVNLMLGQAQECLLEKSMLDNRKSFLVARISAQVVDYYKEACRALENPDTASLLGRIQKDWKKLVQMKIYYFAAVAHLHMGKQAEEQQKFGERVAYFQSALDKLNEAIKLAKGQPDTVQDALRFTMDVIGGKYNSAKKDNDFIYHEAVPALDTLQPVKGAPLVKPLPVNPTDPAVTGPDIFAKLVPMAAHEASSLYSEEKAKLLREMMAKIEDKNEVLDQFMDSMQLDPETVDNLDAYSHIPPQLMEKCAALSVRPDTVRNLVQSMQVLSGVFTDVEASLKDIRDLLEEDELLEQKFQEAVGQAGAISITSKAELAEVRREWAKYMEVHEKASFTNSELHRAMNLHVGNLRLLSGPLDQVRAALPTPALSPEDKAVLQNLKRILAKVQEMRDQRVSLEQQLRELIQKDDITASLVTTDHSEMKKLFEEQLKKYDQLKVYLEQNLAAQDRVLCALTEANVQYAAVRRVLSDLDQKWNSTLQTLVASYEAYEDLMKKSQEGRDFYADLESKVAALLERTQSTCQAREAARQQLLDRELKKKPPPRPTAPKPLLPRREESEAVEAGDPPEELRSLPPDMVAGPRLPDTFLGSATP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.2. The engineered protein is expected to adopt a strand-helix-turn-helix-helix-strand-turn-strand-helix-helix-helix-helix-helix-helix-helix-based conformation.3. Design the protein with a signal peptide incorporated.4. The protein should have a specific function in positive regulation of adherens junction organization, endocytic recycling, cilium assembly and should be localized to early endosome in order to carry out this function efficiently.5. For general function, the protein need meet that Plays a role in ciliogenesis.; May act as a negative regulator of Ras-mediated mitogenic activity; Plays a role in sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs) via its interaction with the ESCRT-I complex (endosomal sorting complex required for transport I), and possibly also other ESCRT complexes6. The designed protein have BRO1 domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MHFSIPETESRSGDSGGSAYVAYNIHVNGVLHCRVRYSQLLGLHEQLRKEYGANVLPAFPPKKLFSLTPAEVEQRREQLEKYMQAVRQDPLLGSSETFNSFLRRAQQEERLRAQLEEKKNVEKLLTLLSTSSKDEDSEEIAKLYQQTSAIRPKLIKLIEKYSQKKDDFTQLNEKFIKARRDYEALLEAQQKEEVSLEVLLSNGQKVLVNVLTSDQTEDVLEAVAAKLDLPDDLIGYFSLFLVREKEDGAFSFVRKLQEFELPYVSVTSLRSQEYKIVLRKSYGELEFKKGDVIAVLEE",
    "response#2": "MHFSIPETESRSGDSGGSAYVAYNIHVNGVLHCRVRYSQLLGLHEQLRKEYGANVLPAFPPKKLFSLTPAEVEQRREQLEKYMQAVRQDPLLGSSETFNSFLRRNESLVPDAYKKILETTMTPTGIDTAKLYPILMSSGLPRETLGQIWALANRTTPGKLTKEELYTVLAMIAVTQRGVPAMSPDAKPYGYLRFDACVADFPEKDCPVVVSAGNSELSLQLRLPGQQLREGSFRVTRMRCWRVTSSVPLPSGSTSSPGRGRGEVRLELAFEYLMSKDRLQWVTITSPQAIMMSICLQSMVDELMVKKPVTVYDQHGFRILFHFARDPLPGRSDVLVVVVSMLSTAPQPIRNIVFQSAVPKVMKVKLQPPSGTELPAFNPIVHPSAITQVLLLANPQKEKVRLRYKLTFTMGDQTYNEMGDVDQFSRVRALFDFQPGALTQRGPSALPYAENTKSLIRQHLLNLISSYTSLDPKTATFTHNDGRSVILLQADGTIPMPFQGVSYNIPVVIWLLESYPQYPPCVYVNPTRDMIIKRPHSNVSPSGLVSLPYLQNWIYPSSNLVDLASHLSAAFSRDPPLYSQRQHLLNLISSYPSLEPKTASFMHNDGRSVNLLQADGTIPMPFHGVTYNIPVIIWLLESYPRHPPCVYVNPTADMIIKRPHAHVTPSGLVSLPYLQNWVYPSSNLVDLVSDLSAAFARDPPGYLRFDACVADFPEKDCPVVVSAGNSELSLQLRLPGQQLREGSFRVTRMRCWRVTSSVPLPSGSTSSPGRGRGEVRLELAFEYLMSKDRLQWVTITSPQAIMMSICLQSMVDEL",
    "response#3": "MHFSIPETESRSGDSGGSAYVAYNIHVNGVLHCRVRYSQLLGLHEQLRKEYGANVLPAFPPKKLFSLTPAEVEQRREQLEKYMQAVRQDPLLGSSETFNSFLRRAQQEESLVPDAYKKILETTMTPTGIDTAKLYPILMSSGLPRETLGQIWALANRTTPGKLTKEELYTVLAMIAVTQRGVPAMSPDALNQFPAPHLNGLGYLRFDACVADFPEKDCPVVVSAGNSELSLQLRLPGQQLREGSFRVTRMRCWRVTSSVPLPSGSTSSPGRGRGEVRLELAFEYLMSKDRLQWVTITSPQAIMMSICLQSMVDELGELQFRKGDVIAVLERKXXXGGTSDDDVPCPGYLFEEIAKISHESPGSSQCLLEYLLSRLHSSSGHGKLKVLKILLYLCSHGSSFFLLILKRNSAFIQEAAAFAGPPDPLHGNSLYQKVRAAAQDLGSTSQTGIFPLNYVEKNHEDALTQRGPSALPYAENTKSLIRQHLLNLISSYTSLDPKTATFTHNDGRSVILLQADGTIPMPFQGVSYNIPVVIWLLESYPQYPPCVYVNPTRDMIIKRPHSNVSPSGLVSLPYLQNWIYPSSNLVDLASHLSAAFSRDPPLYSQESLVPDAYKKILETTMTPTGIDTAKLYPILMSSGLPRETLGQIWALANRTTPGKLTKEELYTVLAMIAVTQRGVPAMSPDALNQFPASYGYLRFDACVADFPEKDCPVVVSAGNSELSLQLRLPGQQLREGSFRVTRMRCWRVTSSVPLPSGSTSSPGRGRGEVRLELAFEYLMSKDRLQWVTITSPQAIMMSICLQSMVDELMVKK"
  },
  {
    "reference": "MTESIAHYIARFLGEAGVKRIWGVTGDSLNGLSDSLHRLGEIRWMGTRHEEAAAFAAGAEAHITGELAVCAGSCGPGNLHLINGLFDCQRSHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSVYCELVTNPDQMPRVLEIAVRTAIAKRGVAVIVLPGDVALKPMPDDVDIRWREPELGRLVPSDGQVDALADMLSGSSKVTLLCGAGCAEAHDEVVALAERLKAPIVHALRGKEFVEYDNPYDVGMTGLIGFSSGYHAMGAADTLLILGSSFPYRAFYPTGARIAQVDSRPESLGAHTQIDLGIVGDVKATVSALLKKLPEKSDDGFLKTALEHYAKARKGLDDLATPDGEKGQIHPQYLARLVSEKAADDAIFTCDVGTPTVWAARYVTMNGKRRLVGSFNHGSMANALSQGIGAQGIDRNRQVVALCGDGGFAMLMGDILSVRQLDLPLKIIIFHNRSLGFVAMEMKAGGYISDDTDLNSPDFVKVAEAIGIKGIRVDDAKALPAALDEAFATPGPVLVDVMTAKQELAMPPQITLEQAKGFSLYMMRAILSGRGDELIELAKTNWR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have pyruvate dehydrogenase (quinone) activity, lipid binding, magnesium ion binding to facilitate pyruvate catabolic process.2. The designed protein have Thiamine pyrophosphate enzyme central, Thiamine pyrophosphate enzyme N-terminal TPP-binding, Thiamine pyrophosphate enzyme TPP-binding domains with specific sequence motifs that are crucial for its activity.3. The designed protein should have a binding site that is specific to the target FAD ligand.4. The protein must exhibit the following characteristics: A peripheral cell membrane enzyme that catalyzes the oxidative decarboxylation of pyruvate to form acetate and CO(2). The designed protein sequence is ",
    "response#1": "AEAAALLNGAKKVTILAGAGVEGAHDDVIALADLLAAPIVHALRGKEFIEYDNPFDVGMTGLLGFASGYRAMEAADLVLVLGSDFPYQQFYPSRATTVQVDIRGAQLGRRHPVDLGLVGDVGDTARSLGELAEAPRAREAAGILNAAGKVTILAGAGVEGAHDEVVALADALAAPIVHSMRGKEFIEYDNPFDVGMTGLLGFSSGYRAMEDCDALLMLGTDFPYQQFFPSKAKIIQVDLRGEQLGRRVPLDLGLVGDVRDTAAALLAPLADPAAEGADLDALAHLLRDSRRTTLLCGRGCAGAHDQVVRLAETLKAPVVHALGGKEYIEYDNPYDVGMTGLIGFSSGYHAMQNCDTLVMLGTDFPYRQFYPTDARVVQVDVRPENLGRRTRLDLGLVGDVAATLDAIEALAGLLRDAKKVTLLCGRGTKGAHAELLELAETLKAPIVHALGGKEYVEYDNPYDVGMTGLIGFSSGYQAMKDCDVLLMLGTDFPYRQFYPEQAKIAQIDLRPENLGRRCRLELGLVGDVKTSLRALAPLAAPARDALAHLLRDSRRTTLLCGRGCAGAHDQVVRLAETLKAPVVHALGGKEYIEYDNPYDVGMTGLIGFSSGYHAMQNCDTLVMLGTDFPYRQFYPTDARVVQVDVRPENLGRRTRLDLGLVGDVAATLDALVPLAAPEA",
    "response#2": "LEEAVSVLAGSRRVTILAGSGSADAHDEVVALADRLAAPVVHTLRSKEHLEHDNPFDVGLTGLLGFASGYRAMMEADVLLMLGTDFPYRQFYPQDATVIQVDLRGEHIGRRTRVDLPIVGTVRDTAAAVALITG",
    "response#3": "TAADYLVGALAQAGVQRIFGVVGDSLNGFTDALRRDGSIEWVHMRHEESAAFAAGAEAHLTGQLAVCCGSCGPGNLHLINGLFDCQRSGLPVLAIAAHIPSTEIGIDYFQATHPEALFKDCSHYVELVSNPGQLPQILGRAIRTAVARKGVAVVVMPGDVDLFTPADEPIHPQYLARLVDRVAAEDAVFTADVGSPVVWAARYLTMNGRRRLIGSFNHGSMANALMHALGAQAVDRSRQVVSLSGDGGLAMMLGELLTAVQNDLPVKVVVLNNSSLNFVELEMKAAGYVNYGTGLDNPDFGAVAEAMGLKGLRVERSADLEGALREAFAHEGPALVDVVTDRQEMPIRIEQAKAQGVFMRAVVQALEGR"
  },
  {
    "reference": "MATPPPLRRVAALLLLLVAAASTPTARADLVVTRADRKVDLTSHIVRVLTSLKVENSGPEAVSQFLLAFPNVQAKNLAAIRAFGTEEKVKGPSMVLPIEVVQPSGVPPELTFFSASLSKPLEKGKTLHLDVLTVFTHSVQPFPEEITQAESQLVVYQDSAQYLSPYPVKVQTLSIRLPGGRVESYTKYPNTKLAESELKYGPYEDLPPFSYSPMVVHYENNNPFAVAKEVIREIEISHWGNVQITEHYNIAHGGAKLKGEFSRIDYQSRPYIRGVSSFRHLIARLPPRAHSIYYRDEIGNISTSHLWSDSKKTQLEVEPRFPLFGGWQTTFTIGYGLPLQDFVFNSDGKRFLNITFGSPVEEILIEKLIVKVVLPEGSKDIDISVPFPTKQEQEVKYSHLDISGRPVVVLEKLDVIPEHNLYFQVYYRFNNISLLREPMMLITGFFLLFMACIVYMRTDMSISKNSPSYLAKVQWDEVQSIIQQIQAIFNQCLAAHDKLETSLHELSRSGDVKSCKVARKTADAQFKELAKELKPLLTSLQSSSQSYQIWPKVEELVAKERELQDKLMTRHSTVVDSFEKKLRGQDVENRIAAQQQKVAALRQEVESLLEYISEI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that is capable of catalyzing reactions within the Protein modification, protein glycosylation pathway with high efficiency and specificity.2. For general function, the protein need meet that Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol- pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation; All subunits are required for a maximal enzyme activity.; N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER)3. The protein should be designed to localize specifically to oligosaccharyltransferase complex in order to perform its role in protein N-linked glycosylation via asparagine more effectively.4. The designed protein should contain Lumenal topological domains.5. Incorporate a signal peptide in the protein design.6. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "SDAHRSAAAELFAASPDGSFGDLETTYEAVRTFQILGVEKDKGLIGKACKFAAEKLASSSSSPAKDLFHAARISGVLKCSVDSGVYDDVATRLKAVIKDTNSLLELYYSVGGLLSIKEQGHNVVLPDADNTFHAIKALSQSDGRWRYDTNSAESSTFAAGIALEALSAVISLADSEVDSSMIAVVKNDIVKLFDTIKSYDDGTFYFDEKHVDAAEYKGPITTSASVVRGVTSFAAVASGKLNIPGEKILGLAKFFLGIGLPGSAKDCFNQIESLSFLYDDKVASWWDYGYQTTAMANRTVIVDNNTWNNTHIATVGTAMSSPEKAAWEIFNSLDVKYNSDAHRSAAAELFAASPDGSFGDLETTYEAVRTFQILGVEKDKGLIGKACKFAAEKLASSSSSPAKDLFHAARISGVLKCSVDSGVYDDVATRLKAVIKDTNSLLELYYSVGGLLSIKEQGHNVVLPDADNTFHAIKALSQSDGRWRYDTNSAESSTFAAGIALEALSAVISLADSEVDSSMIAVVKNDIVKLFDTIKSYDDGTFYFDEKHVDAAEYKGPITTSASVVRGVTSFAAVASGKLNIPGEKILGLAKFFLGIGLPGSAKDCFNQIESLSFLYSDAHRSAAAELFAASPDGSFGDLETTYEAVRTFQILGVEKDKGLIGKACKFAAEKLASSSSSPAKDLFHAARISGVLKCSVDSGVYDDVATRLKAVIKDTNSLLELYYSVGGLLSIKEQGHNVVLPDADNTFHAIKALSQSDGRWRYDTNSAESSTFAAGIALEALSAVISLADSEVDSSMIAVVKNDIVKLFDTIKSYDDGTFYFDEKHVDAAEYKGPITTSASVVRGVTSFAAVASGKLNIPGEKILGLAKFFLGIGLPGSAKDCFNQIESLSFL",
    "response#2": "LCAFTLLLIGVLAFSIRLFSVIKYESVIHEFDPYFNFRVTQFLSKNGIYEFWNWFDDRTWYPLGRVIGGTVYPGLTLTAGTIWWLLNSLNIPLSVETVCVFTAPIFSANASWATYLLTKEAKGTGAGLMAAAILAMVPSYISRSVAGSYDNEAVAIFALIFTFYLYVKTLNTGSLFYATLNALSYFYMVCSWGGYTFIINLIPIHVLLCIVTGRYSSRLYIAYAPLVILGTLLAALVPVVGFNAVMTSEHFASFLVFIILHVVALVYYIKGLLTPRLFKVAMTLVITVGLAVCFAVIAILIALVASSPTKGWSGRSLSLLDPTYASKYIPIIASVSEHQPPTWPSYFMDINVLAFLIPAGIISCFLPLSDASSFVVLYLVTAVYFSGVMVRLMLVLAPAACIFMLSNTNMMYNSVLTIVVLALAWFVGFASRLFAIVRFESIIHEFDPWFNYRATHHMVQHGFYKFLNWFDERAWYPLGRIVGGTVYPGLMVTSGLIHWILDSLNFHVHIREVCVFLAPTFSGLTAIATYLLTKELWSPGAGLFAACFIAISPGYTSRSVAGSYDNEGIAIFALQFTYYLWVKSLKTGSIMWASLCALSYFYMVSAWGGYVFIINLIPLHALALIIMGRYSSRLFVSYTSFYCLATILSMQVPFVGFQPVRTSEHMPAFGVFGLLQIVALMHYARNRITRQQFMTLFVGGLTILGALSVVVYFALVWGGYVAPFSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHITAAVFPVGLWYCIKKVNDERVFIILYAVSAVYFAGVMVRLMLTLTPAVCVL",
    "response#3": "LTIVVLALAWFVGFASRLFAIVRFESIIHEFDPWFNYRATHHMVQHGFYKFLNWFDERAWYPLGRIVGGTVYPGLMVTSGLIHWILDSLNFHVHIREVCVFLAPTFSGLTAIATYLLTKELWSPGAGLFAACFIAISPGYTSRSVAGSYDNEGIAIFALQFTYYLWVKSLKTGSIMWASLCALSYFYMVSAWGGYVFIINLIPLHALALIIMGRYSSRLFVSYTSFYCLATILSMQVPFVGFQPVRTSEHMPAFGVFGLLQIVALMHYARNRITRQQFMTLFVGGLTILGALSVVVYFALVWGGYVAPFSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHITAAVFPVGLWYCIKKVNDERVFIILYAVSAVYFAGVMVRLMLTLTPAVCVLFLMTWLNTLSFSDAHRSAAAELFAASPDGSFGDLETTYEAVRTFQILGVEKDKGLIGKACKFAAEKLASSSSSPAKDLFHAARISGVLKCSVDSGVYDDVATRLKAVIKDTNSLLELYYSVGGLLSIKEQGHNVVLPDADNTFHAIKALSQSDGRWRYDTNSAESSTFAAGIALEALSAVISLADSEVDSSMIAVVKNDIVKLFDTIKSYDDGTFYFDEKHVDAAEYKGPITTSASVVRGVTSFAAVASGKLNIPGEKILGLAKFFLGIGLPGSAKDCFNQIESLSFLL"
  },
  {
    "reference": "MSSYQSEYNADEQAAIDKEFTRLANNKSIYLDHAGTTLYAESQVTAAAEQLQRDVICNPHTCRVTGDYVDQVRFKLLEFFNTKEDEYHVIFTANATAALSLVAENFDFGRQGNFHYCQENHTSVLGMRERVQARAMYMLKEEEITGMASVPSAANGVSGSSPGDNSLVTFSAQCNFSGYKIPLAAIAGIQKQGLPHGLGKKISGEAPQTTDNNNYYVCLDAASFVATNPLDLQRYRPDYVCISFYKIFGYPTGVGALLVSRRGAEAFRKKRNFFGGGTINYAYPHAMDHQLREVFHQRYEDGTLPFLSIVGLLEGFRTLERLVPRRSVNGGDVATMERISRHVHGLAQHLEKQLRQLKYPNGQPLIELYNRVGYEERHRQGGIVAFNVRTDAGPFVGFGEIACVAALQGILLRTGCFCNIGACQRYLGLDETMMDAIYKRAGRICGDYYDLIDGQPTGAVRVSFGYMTRRQDVDELLKMLHLSYLATKPQQRLQLIEEQAGELPKALKERAQRLRPQLLQLAIYPVKSCAAFKIEEGGGSGGGGSGGTWPLTAQGLQYDREWMIVDMNGMAVTQKRCSELCLIRPLIRDDQLVLHFGDSPDGVSLPLSLADQAENSSRCRSKVCRQPVEGLDCGDEVALWLSQHLGLEGLRLLRQSSQRSTTNGVRQQQKLSLVNQAQFLLVNRSSVRSLQFEESLDETVDRFRANIIIDTGSAFEELSYKQLTIGQVQFQVEGPCQRCDMICINQRTGERSPETLTTISRLQSGKMR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Sulfation of molybdenum is essential for xanthine dehydrogenase (XDH) and aldehyde oxidase (ADO) enzymes in which molybdenum cofactor is liganded by 1 oxygen and 1 sulfur atom in active form..2. The protein must bind to pyridoxal 5'-phosphate in order to perform its enzymatic function.3. A protein with lyase activity, Mo-molybdopterin cofactor sulfurase activity, pyridoxal phosphate binding functions is required to enable Mo-molybdopterin cofactor biosynthetic process. The designed protein sequence is ",
    "response#1": "LVLIDAAKGCATNPPNLSMFKADFVVFSFYKLFGYPTGLGALIVRKDAAKLMKKTYFSGGTVTAAIADVDFFKRREGVEEFFEDGTISFLSITAIQHGFKIINMLTTSSIFRHTTSIAAYVRNKLLALKHENGEFVCTLYGLLSSEMGPTVSFNMKRPDGTWYGYREVEKLATLAGIQLRTGCFCNPGACAKYLGLSHLDLLSNIEAGHVCWDDRDILHGKPTGAVRVSFGYMSTFEDLRLLRQSGQRNSSKDQQKLSLVNQAQFLLLNKSSVRSLQFEEPLDETVDRFRANIIIDTGSAFEELTYKALSIGGIQFQVEGPCQRCDMICINQRTGERSPETLTTISRLQKGRMRFGIYITRIPQDTKELEAKEHMTCGDVVLVRLNFVNEAQFLLISEESIKDLNSRLKSNGRRRNGGQAVQVGVMRFRPNLVASSGEPYAEDGWSNINIGGKYFMSLGGCNRCQMININPEAGEVQRFTEPLATLAGYRRAKGKIMFGILLRYENNTKTESDTWIRVGEEL",
    "response#2": "LVLIDAAKGCATNPPNLSMFKADFVVFSFYKLFGYPTGLGALIVRKDAAKLMKKTYFSGGTVTAAIADVDFFKRREGVEEFFEDGTISFLSITAIQHGFKIINMLTTSSIFRHTTSIAAYVRNKLLALKHENGEFVCTLYGLLSSEMGPTVSFNMKRPDGTWYGYREVEKLATLAGIQLRTGCFCNPGACAKYLGLSHLDLLSNIEAGHVCWDDRDILHGKPTGAVRVSFGYMSTFEDLRLLRQSGQRNSSKDQQKLSLVNQAQFLLLNKSSVRSLQFEEPLDETVDRFRANIIIDTGSAFEELTYKALSIGGIQFQVEGPCQRCDMICINQRTGERSPETLTTISRLQKGRMRFGIYITRIPQDTKELEAKEHMTCGDVVLVY",
    "response#3": "NSDSQSHSCINKNGSPGMCRDVGARLNFVNEAQFLLISEESIKDLNSRLKSNGRRRNGGQAVQVGVMRFRPNLVASSGEPYAEDGWSNINIGGKYFMSLGGCNRCQMININPEAGEVQRFTEPLATLAGYRRAKGKIMFGILLRYENNTKTESDTWIRVGEEIIPA"
  },
  {
    "reference": "MELSSAEAVSTRFLGLTQRSVSEDLGCRRGEFSRKHYGSVELLISSDADGAIQRAGRFRVENGSSDENTGYTPGTWHRTDVHLENPEYYTRWYFKYFLGKVHQNYIGTDSEKNPFFLSVVLSDQNNQRVPQYHSILWRKTGTQKICLPYSPTKTLSVKSILSAMSLEKFEKGPREIFHPDIQKDLLVLEEQEGSVNFKFGVLYAKDGQLTDDEMFSNETGSESFQRFLHLLGDTITLKGWTGYRGGLDTKNDTTGTFSIYTVYQGHEIMFHVSTMLPYSRENKQQVERKRHIGNDIVTIVFQEGEETAPAFKPSMIRSHFTHIFALVRYNKQNDSYRLKIFSEESVPLFGPPLPSPPVFTNHQEFRDFVLVKLINGEKATLETPTFSQKRQRTLDMLIRSLYQDLMPDLHKNMLNRRSFSDVLPDSPKSTRKKEEARQAEFVRLGQALKLKTGVKGDAPTTLATTSFCKKEPWESQSFCSNFPHEVVCADSWGQSLLVSTDAGILLIDDGQTTVQVFDKTLQIKQMHVLEALDYLIARTDKGKDSRLLVFRLSSVQKDIETKQVIRSKYDCRENKLERTKGCHLYAINTHHSSELRIVVAIRNKLLLITKKYNPCNSLTGSSLSLSESPVEEFQYIREICLSDPPVVMTLVDGPTGDSDNMICVAYRHQFDLVNESTGESYRLHHIEANKVNFVAAIDVYEDGEAGLLLCYNYVCQYRKVYPFNGGSPLIQPSAYDFHFSWNQVPYAVVCAFPYILAFTTDSIEIRLV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be involved in activation of GTPase activity, regulation of small GTPase mediated signal transduction by localizing to cytoplasm and exhibiting GTPase activator activity.2. The designed protein must have at least one Rap-GAP, CNH domain. The designed protein sequence is ",
    "response#1": "CDLQEHLQHSGQEVPQVLKSCAEFVEEYGVVDGIYRLSGVSSNIQKLRQEFESERKPDLRRDVYLQDIHCVSSLCKAYFRELPDPLLTYRLYDKFAEAVGVQLEPERLVKILEVLRELPVPNYRTLEFLMRHLVHMASFSAQTNMHARNLAIVWAPNLLRSKDIEASGFNGTAAFMEVRVQSIVVEFILTHVDQLC",
    "response#2": "DKRYKRIKRLINNEQFCKNVILQKLVDSTKDIIENKVHGNILAATISLWTHAAEKHKEMSSDDRALLFNELSKVPNSEYYPATVDALIVITSQGERTESFSNDILKVLSEWLTDLFTIIDDMRAQSRKSFKGSAELRNYTRCFRQIILLLTNMFRVGFNLFDEMEVSSMLANCVWVSKSTTDEEDIALVFSLLNCIISYGRISSAVLYSIVEVVCRAKFGLVASSQSAQQIVEKLLKTATKYEALNSLKRIMEETDEGSYIAIMGGIQIVSALFTDIPRSVYCTRGMLLGFLQRSLKNKSSRIALETTRSLYNALERRSFVEVLYADEWISLLDLLVEISSSLPKSLNYRPGTWQHIEPNIIESIASYQLQHLLLFNEILKPDSNHFVLEKFHEFLKINFSYLTPTLSQKLFTVLDLRSQLPYSEGWLEDQTILLKSYFDTSFSLDFHVYLIR",
    "response#3": "STVPQFVRQCIKAVERRGLEVDGIYRVSGNLATIQKLRFVVNQEEKLNLDDSQWEDIHVVTGALKMFFRELPEPLFPYCFFEQFVEAIKIQDNATRIKAVKTLVKKLPRPNYDTMKVLFEHLKKIAAKESVNLMSTQSLGIVFGPTLLRPEKETGNMAVHMLYQNQIVELMLSEYR"
  },
  {
    "reference": "MVFSKLQELRYGYEPKMPQALQAKPEEIKMIIGEPTDALQDKDELKKLFKNSYGLPLVHFEKGKSETFNSKALKIGFILSGGQAPGGHNVISGLFDSLKKANSKSELIGFLGGPSGLVDGKYITLTKDKIDEYRNTGGFDMIGSGRTKIETDEQVESSIKTVNQLSLDAIVIAGGDDSNTNAALLAEKFLNAGLKTKVIGIPKTIDNDLKGDLIETSFGFDTAAKTYSEVIGNICRDTNSAKKYWHFIRVMGRSASHIALECGLQTQANICLIAEEVAEKNMTLKEVTNYIADIIEKRAKNGENFGVIIVPEGLIEFIPEVKKLIAELNDVLALKKDDFAKRKTYTEQQVWLTENISKSAKKTFEDLPEEISRQLLIDRDPHGNVQVSRIETEKLLIATVKHELERRQKAGTYNGKFSSYAHFFGYEGRCAFPSNFDADYCYSLGVTAFFLIANGRTGYIAAIKNLSKPRTEWLAYGIPVTMLMNMERRSGKEKPVIKKSLVSLDDPAFKKLAEGRKTWEVTTSYIYPGAIQYFGPSELVDPPTKTVLLNAMG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should interact with enzymes and substrates within the glycolysis, Carbohydrate degradation pathway to enhance or regulate its activity.2. The designed protein must have at least one Phosphofructokinase domain.3. The protein should be able to bind Mg(2+), diphosphate, substrate ligand in a variety of conditions4. The ideal protein would be one that plays a critical role in fructose 6-phosphate metabolic process through ATP binding, metal ion binding, diphosphate-fructose-6-phosphate 1-phosphotransferase activity within cytoplasm.5. The protein must exhibit the following characteristics: Consistently, PPi-PFK can replace the enzymes of both the forward (ATP- PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.; Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis; Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis.6. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for beta-D-fructose 6-phosphate + diphosphate = beta-D-fructose   1,6-bisphosphate + H(+) + phosphate. The designed protein sequence is ",
    "response#1": "MPKIGILTGGGDCPGLNATIRAVVRKGIKDNNYKMYGILEGWKGLIEGNIKELDMFSVSGILPRGGTILGTSRTNPFGEGKEKDQERILDNIKALGLYAIVAIGGDDTQGVAYKLSEMGVNVIGIPKTIDNDILCTDYTFGFDTAVNIVMDAIDRLHTTAESHNRVMVVEVMGRHAGWIATFAGIAGGADIIMIPEKPLDMEDVYKSIIRRHKSGKNFSIVVVSEGVKISVADREKEGFALQSNNKDDFGHVMLGGIGSVLANLIEKRTGFETRNTVLGHTQRGGSPTAFDRILSTRFGVAAMEAVLSGNPACMVALKGTRVELVPLTEVLNKRRVVDEAMFRAAEVFFG",
    "response#2": "MKIGILTGGGDCPGLNATIRAVVRKGIKDNNYKMYGILEGWKGLIEGNIKELDMFSVSGILPRGGTILGTSRTNPFGEGKEKDQERILDNIKALGLYAIVAIGGDDTQGVAYKLSEMGVNVIGIPKTIDNDILCTDYTFGFDTAVNIVMDAIDRLHTTAESHNRVMVVEVMGRHAGWIATFAGIAGGADIIMIPEKPLDMEDVYKSIIRRHKSGKNFSIVVVSEGVKISVADREKEGFALQSNNKDDFGHVMLGGIGSVLANLIEKRTGFETRNTVLGHTQRGGSPTAFDRILSTRFGVAAMEVAILVGGGPAPGINGVIRSITIEAVNSGLRVIGIYDGFKWLARGDSSRTTELHTENVSRIHFLGGSILRTSRDNPTETPAKMEATVRALTQLEVDYLITIGGDDTAYSSQRLHELVGVQIQIAHVPKTIDNDLPLPGDMPTFGYETARHVGAEIVRTLMEDAQTTNRWYYVVSMGRKTGHLALGIGKAAGATLTIIGEEFRNRKISFTTVCDMLETSAIKRMAMGRPYGVAVLAEGILDRIDKNELMRVADLDYDAHGNIRFSEIDLGRMLKNEVSRRMKERGIPATLVAKDIGYELRCASPIPFDREYVQDLGYAAVRYLLEGEEDVMPALEGIRIRVVDLKEAIGRTKRVKEDEYDLAKRYIRHY",
    "response#3": "KIGILTGGGDCPGLNATIRAVVRKGIKDNNYKMYGILEGWKGLIEGNIKELDMFSVSGILPRGGTILGTSRTNPFGEGKEKDQERILDNIKALGLYAIVAIGGDDTQGVAYKLSEMGVNVIGIPKTIDNDILCTDYTFGFDTAVNIVMDAIDRLHTTAESHNRVMVVEVMGRHAGWIATFAGIAGGADIIMIPEKPLDMEDVYKSIIRRHKSGKNFSIVVVSEGVKISVADREKEGFALQSNNKDDFGHVMLGGIGSVLANLIEKRTGFETRNTVLGHTQRGGSPTAFDRILSTRFGVAAMEACAGGDVKVMLPLRERRVGVLTGGGDCPGLNAVIRSVVRKGAQEYAWEFVGIADGWGGAVKDVTRPLDVAAVRGILPLGGTILGSSRTNPLRLDRGIDRIRETLRAHEIDALVVIGGEDTLSVASALSDAGVDLVGVPKTIDNDVGGTDYTFGFDTAVGIATEAIDRLHTTAESHKRTLVVEVMGRHSGWIALHAGVAGGANAILIPEVEFDLDRVCAWVERRFEINYAPIVVVAEGAVPRAGQMVVKDESRDEFGHVRLSGIGDWLARELESRTEKEARTTVLGHVQRGGTPSAYDRWLATRFGLHAVEAILNGEIGCMVALKGTEVVAVPLAAALAATKTVPPELYELAGVFFG"
  },
  {
    "reference": "MSSDEKQRFDSIEEAIEDIRNGKMVILVDDEDRENEGDLVCAAEKVTPEIINFMAKFGRGLICLSLSREKAETLDLGPMVRDNTCAFETAFTVSIEARHGVSTGISAQDRAHTILTAVAPDSKAHDLVRPGHVFPLIAKDGGVLQRTGQTEGSVDLARLAGLSPAGVICEIMNDDGTMARYSELRAFADEHHMKLVTIKDLISYRVHVRKELSIERIAEANLPTRYGGDFKLIGYRSRLDGSEHIALVKGDITPETPTLVRVHSQCLTGDVFGSLRCDCGSQIQTAMKAIEEAGSGVVLYLFQEGRGIGILNKIRAYHLQDEGMDTVEANEALGFAADLRDYGTGAQIIADLGIKKIRLLTNNPRKLVGLSGYGLEIDERVPLHTAECEHNTRYLNTKKDKLGHLL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must have high specificity towards GTP cyclohydrolase II activity, zinc ion binding.2. The Nucleophile; for GTP cyclohydrolase activity, Proton acceptor; for GTP cyclohydrolase activity active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "RMTAVRLPTAYGEFTALAFRETLTGRLHVALVKGEVEGQEDVLVRVHSECLTGDVFHSLRCDCGEQLDEALQTIASEERGVLLYMSQEGRGIGLLSKLQAYELQETGLDTVEANVELGFPPDAREYGIGSQILADLGLTTIRILTNNPRKITGIEGFGLRVSEQVR",
    "response#2": "ITKLPSLFGDFEAIGYQNELDGTEHVALVKGKVGEFKEPVLVRMHSECLTGDAFGSLRCDCRPQLEAALSRISKEGEGIVVYLRQEGRGIGLINKLKAYSLQDGGLDTVEANEKLGFPADLRNYGVGAQILSDLGVHKLKLLTNNPRKIAGLGGYGIKVETRR",
    "response#3": "IAVDMPTEHGMFRLKAFKQKNTNETHLALYKGEWEENEPVLVRVHSSCVTGDIFGSYRCDCGNQLHSAMQMVEKAGKGVVLYMNQEGRGIGLLNKLKAYKLQEEGLDTVEANEKLGFHMDQRDYGVGAQILRKMGVTKLKLITNNPKKRIGLMGYGLEIVENR"
  },
  {
    "reference": "MLMQVEKKKLLSQADIPTKFDPINYLGEYLMRHNTHYIKDPGVSGYQRVMKEITEELKTHVPDTINNRISKMKEKVKEKREQREYISTVKVKVAGMRKQALEEQFNEWVLNPKGMIPIVVYISSYITDLKSEVFEEFLKHLCHSAEEFREIILTDMRRQMFAELFLQCDSGKVMALDRQRTLALLEAFYDQCSSTTRSLLRNPRQWPFVEFEEIELIEFWGDMDIKKHIYEDFDALLLKMNMLVAEKLAGKLAENKDLPQQQRDQELSSDSTTEPETATQLTSQQRSRRVSLTGQGQGKGPRKTSASKQGASRGSVAEQGSRRSSGVNQTQQRGSVAEQGSRRSSAVEQTQQRGSVAEQGSRRSSTVEQTRQRGSVAEQGSRRSSTVEQTQRRGSVAEQGSRRSSGVNQTQQRGSVAEQGSRRSSAVEQTQQRGSVAEQGSRRSSAMEQEPQTAQDPNSDSLPEQESHRGSITEGSHRGSISEPGQRRASVTGQRRKSSVDDSGSAGSRRGSGTDQGQHRGSVGQRKGSGERKMSASEYGPHQESITEEPLTASEPGPQIDTIQELDEDSTPQLEDDSALKESKTSELTKIETQEEKPLLLINEEQVPRDSKQPEVPTSSKKERPSGSPKKGRLSGTSKRDSQKDKACEPKPQHVEGKKWSGEFLICDWKIKHVKSEEEEQAKLICDDTRFTDLHATIRNFQTYKGIKGRSAFNGVSLDLLQFVQLLETFVGEDTSFSLSKDLASFFQKNYSETKQEKIKALEQARQN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must have high specificity towards calcium ion binding. The designed protein sequence is ",
    "response#1": "VLIISGSLGKTGAAVMAARAALKTGAGLVTAATPASCLPIIARSMAELMTEPLEETPEKTIAARAADRAAALARGKEAVLFGPGISTHPSTAGFLDSLLPKLKGPVVIDADGLNLLGLRPDLLARLPRPAVLTPHPGEFARLTGLPTAEVLKRRLELAPAFASEHNVYLVLKGYRTLVAAPDGRVFINPTGNPGMATGGSGDVLSGILASLLAQFKDPLLAALAGVYVHGLSGDLAAESLSEKALLAGDIIRFLR",
    "response#2": "DTHKGTYGHVLIISGSLGKTGAAVMAARAALKTGAGLVTAATPASCLPIIARSMAELMTEPLEETPEKTIAARAADRAAALARGKEAVLFGPGISTHPSTAGFLDSLLPKLKGPVVIDADGLNLLGLRPDLLARLPRPAVLTPHPGEFARLTGLPTAEVLKRRLELAPAFASEHNVYLVLKGYRTLVAAPDGRVFINPTGNPGMATGGSGDVLSGILASLLAQFKDPLLAALAGVYVHGLSGDLAAESLSEKALLAGDIIRFLG",
    "response#3": "RRKSDTHKGTYGHVLIISGSLGKTGAAVMAARAALKTGAGLVTAATPASCLPIIARSMAELMTEPLEETPEKTIAARAADRAAALARGKEAVLFGPGISTHPSTAGFLDSLLPKLKGPVVIDADGLNLLGLRPDLLARLPRPAVLTPHPGEFARLTGLPTAEVLKRRLELAPAFASEHNVYLVLKGYRTLVAAPDGRVFINPTGNPGMATGGSGDVLSGILASLLAQFKDPLLAALAGVYVHGLSGDLAAESLSEKALLAGDIIRFLPKALR"
  },
  {
    "reference": "MPEGKTDMERIGLFSEMGYTSIGDKYAAPGSKPFNESASKNRQMLPGGSKSMANMLGGYFDGQFKRVFEGESYSDPFKQRRQHRMQQSKKNLGKPFLPSSGEKKRSGLGSFYGTLGGPVVAFSAELKSRKAYTAPGKNFYTNPPKDGSGYGYPSVTIGKPYPYSSENYDISRELIKKEIEHHKSKLKGGAFKLNLHPKDYFEPNPYYTDKTLPPLKVHSQKKETEKPFKPSSPAKEAGGMKAGTFDPYPTHSNDPYTAKPSKTPVKERKVFHPPGGPKTYPVHSILTSNVIKSVTALNYKTVNLASY",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC",
    "response#2": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC",
    "response#3": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC"
  },
  {
    "reference": "MASSFARDGSPSRGRSTSPRPRIPEESLEASLMDGLPDTRQLQAFGRKVTATAGSLIGTEGVGQHYQNALGELHRELRRPMLQRSVFSFAQTTPREIVRSRISVPEIQQRALAYVPDDMLANIPEDNNEFSLFQGFQATLPDEPETIKKKGKTHNARGQRLIGGELEDDEYSKLPPSMQRLQKQKHSMSHQLEMMGVRKHMCVAEIHEIDNKIANLNTMRKMVLDRLAGLEIQEEELAQDLLGVDNEIEDLQEELDDAAALAPPKEDSRPTTSGSEAVSETFMSESIYQKISPKSKNRGKKPIRRPSMRVLHEHLESGSKIKELPAHNDSITAMDFDAPWGTLVTASLDDTVRVWDLNAGRCIGMLEGHLSSVRCLQVEESIVATGSMDATIRLWDLSRAEYAPQDNRVNKRGGEGEGEGDGDAQEDEDGLAFENSSDAPPAPPPTIMQDVPLFTLESHVDEITAIHFKGDTLVSGSADKTLRQWDLVKGRCVQTLDVLWAAAQATATNNASSEWRPTGRSMDASADFVGAIQVFDAALACGTADGMVRLWDLRSGQVHRSLVGHTGPVTALQFDDVHLVTGSADRSIRIWDLRTGSIYDAYAYDNPVTSMMFDSRRIVSAAGESVVKVYDKTDGRHWNCGPGVGADEDDNTSHAMIERVRIKDGYLVEGRRDGTVGVWSC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Target a Polar residues compositional bias in the protein's composition for improved properties.2. Include a signal peptide as an essential component of the protein.3. The protein should be designed to localize specifically to mitochondrial outer membrane, mitochondrion in order to perform its role in mitochondrial fission more effectively.4. For general function, the protein need meet that Acts as an adapter protein required to form mitochondrial fission complexes The designed protein sequence is ",
    "response#1": "KLQDRCPSTSLKDIDHLFRVDTGKGLDETFDEFDPIALGVASLAQVHKARLKDSDVWVAVKVQHPSVSLNSPLDLSMTRWVFKAIKTFFPDFKLMWLADEIERSLPQELDFTREAKNSIETKEHFAHLSTSLYVPEVMWSKPRILVMEYVAGARIDNLSFMDEHSISRDLVSVDICHIFNEMIFGKGGHLHCDPHGGNVLIRSKPKNSKSPRNYEIVLLDHGLYRDIPHELQVDYANMWLNIINFNEKNLF",
    "response#2": "MSIDLNWETLTTGPDGIALAERIRDFVHAKFQTVTLPRFIKGVKVHTFEFGSIAPEVELKDICDPLPDFYEDIDDDYVEDDEDEENSENSQTDEENEVAKTLRERRRLDRVERTANGSSHVSTPPAYIDTRYPGLRSMQASGDTGSPFLGVSTPGIPGGTSNLSYFHSQLASGLSGTQTPLAAVAGAHLPQGWPDRPSPSLHLSALRNQSHTPLSHTASERSMTPPQIADLNQQSLREKASVSTLAASSSTTRPATRDGVPEATIPEEHAGESEESTSPPRRFREPKVEDLQTVFRVRYSGNIRLSLTVDILLDYPMPSFVGIPVKLNITGLSFDGVAVLAYIRKRAHFCFLSPEDAYAAIGADEKDADASAGMKMGALLHEIKVESEIGQRENGKQVLKNVGKVEKFVLEQVRRIFEDEFVYPSFWTFLV",
    "response#3": "MSIDLNWETLTTGPDGIALAERIRDFVHAKFQTVTLPRFIKGVKVHTFEFGSIAPEVELKDICDPLPDFYEDIDDDYVEDDEDEENSENSQTDEENEVAKTLRERRRLDRVERTANGSSHVSTPPAYIDTRYPGLRSMQASGDTGSPFLGVSTPGIPGGTSNLSYFHSQLASGLSGTQTPLAAVAGAHLPQGWPDRPSPSLHLSALRNQSHTPLSHTASERSMTPPQIADLNQQSLREKASVSTLAASSSTTRPATRDGVPEATIPEEHAGESEESTSPPRRFREPKVEDLQTVFRVRYSGNIRLSLTVDILLDYPMPSFVGIPVKLNITGLSFDGVAVLAYIRKRAHFCFLSPEDAYAAIGADEKDADASAGMKMGALLHEIKVESEIGQRENGKQVLKNVGKVEKFVLEQVRRIFEDEFVYPSFWTFLV"
  },
  {
    "reference": "MQLVILRVTIFLPWCFAVPVPPAADHKGWDFVEGYFHQFFLTKKESPLLTQETQTQLLQQFHRNGTDLLDMQMHALLHQPHCGVPDGSDTSISPGRCKWNKHTLTYRIINYPHDMKPSAVKDSIYNAVSIWSNVTPLIFQQVQNGDADIKVSFWQWAHEDGWPFDGPGGILGHAFLPNSGNPGVVHFDKNEHWSASDTGYNLFLVATHEIGHSLGLQHSGNQSSIMYPTYWYHDPRTFQLSADDIQRIQHLYGEKCSSDIP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein's functional design necessitates the inclusion of a Cysteine switch motif.2. A protein that contributes to proteolysis, extracellular matrix organization by functioning in extracellular matrix, extracellular region with zinc ion binding.3. The protein must bind to Ca(2+) in order to perform its enzymatic function.4. The protein must exhibit the following characteristics: Is also able to activate progelatinase B.. The designed protein sequence is ",
    "response#1": "VPDSIDYRKKGYVTPVKNQGQCGSCWAFSSAGALEGQLKKKTGKLLALSPQNLVDCVTENYGCGGGYMTTAFQYVQQNGGIDSEDAYPYVGQDESCMYNATAKAAKCRGYREIPVGNEKALKRAVARVGPISVSIDASLASFQFYSRGVYYDENCDRDNVNHAVLVVGYGTQKGSKHWIIKNSWGESWGNKGYALLARNKNNACGITNMASFPV",
    "response#2": "TERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDC",
    "response#3": "PSHIDAYTPSSSFSYAFSGSQVYTISGTKVTKVQSIHDLFPSAPTPVNAALWNPISGSMLLFSSNRVYSYYFSNIRQIFQMDSGFPKTLPSDLGFSVSGALRWINGHQILMSSGDEFAVYDEFWNQVTLKNRISSYFPNLPRGVKGVESPAGSVITAFTSNQVFEYNSRTKSIGRQSGFSSYC"
  },
  {
    "reference": "MASFQDRAQHTIAQLDKELSKYPVLNNLERQTSVPKVYVILGLVGIYTFLVFFNIAGEFLVNFAGFLIPGYYSLNALFTSGKADDTQWLTYWVVYALLTVVESAINAAYWFPFYYIFKFVLILWMSLPQTNGAQVVFHSFLQPVLGRFFTSGSTSANLRAQADAASKSQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.3. The designed protein should have a specific function within the endoplasmic reticulum membrane.4. The protein must exhibit the following characteristics: Required to generate and maintain the structure of the tubular endoplasmic reticulum network and the vacuole; Involved in membrane/vesicle trafficking.; Induces high curvature in membranes and causes membrane tubule formation. The designed protein sequence is ",
    "response#1": "HVLRIHVLEAQDLIAKDRFLGGLVKGKSDPYVKLKLAGRSFRSHVVREDLNPRWNEVFEVIVTSVPGQELEVEVFDKDLDKDDFLGRCKVRLTTVLNSGFLDEWLTLEDVPSGRLHLRLR",
    "response#2": "HVLRIHVLEAQDLIAKDRFLGGLVKGKSDPYVKLKLAGRSFRSHVVREDLNPRWNEVFEVIVTSVPGQELEVEVFDKDLDKDDFLGRCKVRLTTVLNSGFLDEWLTLEDVPSGRLHLRLR",
    "response#3": "ILVVYLDRAQDLPLKKGNKEPNPMVQLSIQDVTQESKAVYSTNCPVWEEAFRFFLQDPQSQELDVQVKDDSRALTLGALTLPLARLLTAPELILR"
  },
  {
    "reference": "MENNPMNAGGEAKCPFSGATAKATQTAGGGTRNRDWWPNQLKLNILRQNSMLSNPMDASFDYAEAFKSLDLAAVKKDLTDLMTDSQDWWPADYGHYGPFFIRMAWHAAGTYRTGDGRGGAGAGTLRFAPLNSWPDNTNLDKARLLLLPIKQKYGNKLSWADLMVLTGNVALESMGFKTFGFGGGRADVWEPEEDIYWGSEREWLGDKRYSGDRDLENPLAAVQMGLIYVNPEGPNGNPDPLASARDIRETFARMAMNDEETVALIAGGHTFGKTHGAADPGKYVGVEPAAAGLEEQGLGWKNSFGTGNGEYTITSGLEGAWTTTPTKWSNNYFENLFGFEWELTKSPAGAHQWKPKNNAGEGTVPDAHNPNKKHAPFMLTSDIALRVDPAYEKISRKFYENPDLFADAFARAWFKLTHRDMGPKSLYLGSEVPTEELIWQDPIPAATYKMIDATDIEALKAQILATGLSVSELVSAAWASASTFRGSDKRGGANGGRIRLTPQKYWEANNPAQLSKVLDALEGVQKAFNAAAGDKQVSMADLIVLGGCAAIEKAAKAAGHSITVPFTAGRTDASQEQTDVESFAVLEPIADGFRNYAKKRYTVSAEDLLVDKAQLLTLTAPEMTVLVGGLRVLNANSNQSQHGVFTHQPEVLTNDFFVNLLDISQTWAPTNDHKDSFEARDRKTGDIKWTGTRVDLVFGSNSELRAIAELYTYTGGQEKMVKDFVAAWNKVMNLDRFDVK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit catalase activity. The designed protein sequence is ",
    "response#1": "AFARAWFKLTHRDMGPRARYLGADVPAEELLWQDPIPALDHTLIDENDIAALKAKVLESGLSVSKLVSVAWASASTFRGSDKRGGANGARIRLAPQKDWRVNNPAQLSNVLDVLEGIQSVFNATQTGDKKISLADLIVLAGCAAVEKAAKDAGHNITVPFAPGRMDASQEQTDVESVGYLEPQADGFRNYRKANYPASTEALLIDKAQLLTLTAPELTVLVGGMRVLNTNFDGSAHGVFTQTPGKLTNDFFVNLLDMNTAWKPATEDKELYQGYNRATTKPTWTATRADLVFGSNSELRAIAEVYGS",
    "response#2": "AFARAWFKLTHRDMGPRSRYIGPEVPGEELIWQDPVPAVDHKLIDGQDAAALKKSILASGLSVAQLVSTAWASATTYRNSDMRGGTNGARIRLAPQKDWEVNQPDQLSSILQTLETIQQDFNAVAQDGKRVSVADVIVLAGCAGVEQAARNAGREVSVPFEPGRTDASLDQTDVESFAVLEPTADGFRNYLKAKHTVSPEELLIDKAQLMTLTAPEMTVLVGGMRVLNANASQVQDGVFTDRPESLSNDFFVNLLDMGIKWVPTSKECYAFEGRDRATGGLKWSATRVDLIFGSNSQLRAIAEHYAY",
    "response#3": "AFARAWFKLTHRDMGPRSRYIGPEVPGEELIWQDPVPAVDHKLIDGQDAAALKKSILASGLSVAQLVSTAWASATTYRNSDMRGGTNGARIRLAPQKDWEVNQPDQLSSILQTLETIQQDFNAVAQDGKRVSVADVIVLAGCAGVEQAARNAGREVSVPFEPGRTDASLDQTDVESFAVLEPTADGFRNYLKAKHTVSPEELLIDKAQLMTLTAPEMTVLVGGMRVLNANASQVQDGVFTDRPESLSNDFFVNLLDMGIKWVPTSKECYAFEGRDRATGGLKWSATRVDLIFGSNSQLRAIAEHYAK"
  },
  {
    "reference": "MSELSTVARPYAKAAFDFALEQGQLDKWQEMLQFSALVAENEQVVEYITSSLASGQISEIFLQICDDQLDQYGQNFIRVMAENKRLAILPEVFNRFNALRAEYEAIKKVIVVSATELSQAQEEKIAKAMEKRLGQKVRLTTEIDSTLLTGIIIKYDDIVIDGSSRGQLNRLTQALSL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that This protein is part of the stalk that links CF(0) to CF(1); It either transmits conformational changes from CF(0) to CF(1) or is implicated in proton conduction.2. The designed protein should be expressed specifically in the plasma membrane The designed protein sequence is ",
    "response#1": "MGL",
    "response#2": "APNNXVXP",
    "response#3": "MISTLLVL"
  },
  {
    "reference": "MMEKISGGVTAAKGFSAASAAAQIKYQGRTDMAMIYSASPCAAAGTFTRNVVKAAPVIWDRDLVRSGKPVHAVVVNSGIANACTGQTGLDYCLKTAEAAGAALGIPADSVLVGSTGVIGFQLPIDRICAGVEALAGGLGEDLDHGTDAAKAIMTTDTHKKECACSFEIDGKKVTVGGMAKGSGMIHPDMCTMLGYVTTDAAVEASVLQQAVSAIVDETFNMISVDGDTSTNDTLLVLANGEAGNTRITSAEGEAYEALFEALHLVCRDLAMQMAGDGEGATKLIECTVQGADTLENARTLAKSVITSSLTKAAVYGSDANWGRILCALGYAGVEFDPNDLQLYFVDETAASAGEKDSAKAAAAGSGEAEEGGSPRKMLIFTDGAAAAYSEEEATQLLKKEKVTILAQFHMGNASATAWGCDLTYDYVKINADYRS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.2. The designed protein should have a binding site that is specific to the target substrate ligand.3. The Nucleophile active site of the designed protein must be optimized for substrate binding and specificity.4. A protein should be able to manipulate the activity of enzymes in the Amino-acid biosynthesis pathway, either by activating or inhibiting their function.5. The protein can be targeted to cytoplasm and perform glutamate N-acetyltransferase activity, acetyl-CoA:L-glutamate N-acetyltransferase activity. The designed protein sequence is ",
    "response#1": "MLNTIEEAIEDIKDGKLIIVVDDEDRENEGDFVALAEKATPEVINFMTKFGRGLICVPITEERADRLKLEMMVTDNTSLHETAFTVSIDYRHGTTTGISAFDRSHTILALINPDTKPVDLEKKPFYVRYAGHEGIFVRPGHTEASVDLARLAGLYPAGVLCEIIKEDGEMARMPELEIFAKKHNLKIITIADLIRYRRQKETLIEELA",
    "response#2": "MAXKXAXLVXCXNXNXAIXGXGXGFXXXKXTXDXSXKXFXEXDXFXXXXXMXXXGXXXGXVXHWXHXQXMXWXT",
    "response#3": "MTATAPQQTLPSDNALVVLDTGIGGLSVLTEIRRALPQAHLVYAFDNAGFPYGELDEATLVARAVALCDIVLARFPLKAVAXACNTALALXQLRARFSVPLLAMVPAVKPAAXVTXTGRVGVLATPGTVKXAYTAQLXAGFXXDIXXLGSASLVXLAEXRLLGGPVTXAALHXVLAPFXAXXADTAXTXTXXPXVXPXTXAXTXHXVXTHDTTPXTTXT"
  },
  {
    "reference": "MGGKWSKCSGWKAVREKMEKAEPAAEPAADGVGAASRDLEKYGAITSSNINNADCVWLEAQQEEEEEVGFPVRPQVPLRPMTYKGALDLSHFLREKGGLEGLVYSRKRQEILDLWVFHTQGYFPDWHNYTEGPGTRYPLTFGWCFKLVPVDPDRVEEINEGENNSLLHPISLHGMEDPEKEVLQWKFDSSLAFHHKARELHPEFYKNC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should contain at least one PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Dileucine internalization motif; necessary for CD4 internalization motif to achieve its intended function.2. For general function, the protein need meet that Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK13. The ideal protein would be one that plays a critical role in suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II through GTP binding, SH3 domain binding within host cell Golgi membrane, extracellular region, host cell plasma membrane. The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPAVRERMXRAEPAAEGVGAASQDLDKYGALTSSNTPATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLDGLIYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGIRYPLTFGWCYKLVPVDPREVEEANEGENNSLLHPICQHGMEDEHREVLKWKFDSXLARRHMARELHPEFYKDC",
    "response#2": "MGNKWSKSSIVGWPAIRERIRRTDPAAEGVGAASQDLDKHGALTSSNTPATNADCARLEEEQGGKEGVGFPVRPQVPVRPMTYKGAFDLGFFLKEKGGLDGLIYSKKRQEILDLWVYNTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPREVEEDNKGENNCLLHPMSQHGIEDEDREVLQWKFDSSLARRHVARELHPEFYKDC",
    "response#3": "MGGKWSKSSIVGWPAVRERIRQTEPAAEGVGAASQDLDKYGALTTSNTAHNNADCAWLQAQEEEEEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYNTQGFFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPREVEEANEGENNCLLHPMSQHGMEDEEREVLKWKFDSSLARRHIARELHPEYYKDC"
  },
  {
    "reference": "MSFRLSGGSRRICSRTGSGRLSGGGTGFVAGNVCVGSGARSSFSCTLEGISSGGSFCNSGGGLGSGACAGFLGNEHSLLSGNEKVTMQNLNDRLASYLDHVHALEEANADLEQKIKGWYEKCEPGSSREHDHDYSRYFSVIEDLKRQIISATICNASIVLQNDNARLTADDFRLKYENELALHHSVEADTSGLRRVLDELTLCTTDLEIQCETLSEELTYLKKSHEEEMEVLQYTAGGNVNVEMNATPGVDLTVLLNNMRAEYEDLAEQNRKDAEAWFNERSATLQQQISDHEGAATAARNELTELKRNLQTLEIELQSLMAVKHSYECSLAETEGNYCNQLQQIQDQIGVMEEQLQQIRTETEGQKLEYEQLLDVKIFLEKEIDIYCNLLDGEERKSKSTCYKSKGYRPVNSGNQAKDSTEETIVKTVVEELDQIGNLLSLRVHSVEEKSSKISNITVEQRVPSKAP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein have IF rod domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "AYSSSLQPRLRALKGFLRENLYWHYQVLRMTDKARRIIGDLFGAFMADPRLLPPQYQEKARTDKPRAIADYIAGMTDRYAMKEHRR",
    "response#2": "MRRRVLVAATSVVGAVGAGYALVPFVASMNPSARARAAGAPVEADISKLEPGALLRVKWRGKPVWVVHRSPEMLAALSSNDPKLVDPTSEVPQQPDYCKNPTRSIKPEYLVAIGICTHLGCSPTYRPEFGPDDLGSDWKGGFHCPCHGSRFDLAARVFKNVPAPTNLVIPKHVYLNDTTILIK",
    "response#3": "MRRRVLVAATSVVGAVGAGYALVPFVASMNPSARARAAGAPVEADISKLEPGALLRVKWRGKPVWVVHRSPEMLAALSSNDPKLVDPTSEVPQQPDYCKNPTRSIKPEYLVAIGICTHLGCSPTYRPEFGPDDLGSDWKGGFHCPCHGSRFDLAARVFKNVPAPTNLVIPKHVYLNDTTILIE"
  },
  {
    "reference": "MFSEITAAIEDLKAGKVIIVCDDEDRENEGDFLSIAEFVTPETVNFMAKEGRGLICTPISEELAEKLKLLPMVERNTDSRGTAFTVSIDHVETTTGISAFERALTIEKMLDDDARPEEFNRPGHIFPLIAKKGGVLRRAGHTEAAVDLARIAGAKPAGIICEIMKDDGTMARVKDLELIAKKFDIKMISIQQLIEYRLKHDSYIKRETEIKLPTEFGLFKAVGYTNTMDGKEHIALVKGEITEDEPVLLRVHSECLTGDVFGSNRCDCGPQLEAALSQIESEGKGILLYMRQEGRGIGLINKMKAYKLQEEGYDTVEANHKLGFGDDLRDYGVGAQILRDLGVKKMRLLTNNPRKIAGLAGYGLEVVERVPLQMPIKNENEAYLKTKKAKLGHLLKL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be optimized for activity in 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4, riboflavin biosynthesis, Cofactor biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The designed protein should be able to compete with endogenous proteins or inhibitors for GTP, Zn(2+), Mg(2+), D-ribulose 5-phosphate binding.3. The designed protein should have 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, GTP cyclohydrolase II activity, manganese ion binding, GTP binding to facilitate riboflavin biosynthetic process.4. For general function, the protein need meet that Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.5. The Proton acceptor; for GTP cyclohydrolase activity, Nucleophile; for GTP cyclohydrolase activity active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.6. The designed protein have GTP cyclohydrolase II domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "REVVAQLPSEFGDFRIAAYTNLLDGKEHVALIKGEISSDEPILVRVHSECLTGDVFGSLRCDCGPQLHAALEQIEQAGSGVLLYLRQEGRGIGLINKLRAYNLQEQGFDTVEANEKLGFAADLREYGIGAQMLRDLGVRGMRLMTNNPRKIRGLEGYGLKVIERVPHEDVPRVASVRLPTRYGLFNLVAYEDESNGDTHLALCMGEWTEEDPVLTRVHSQCVTGDLFGSIRCDCGDQLHTAMQRVAEEGRGVVLYMKQEGRGIGLLNKLRAYELQDQQGLDTVEANKALGFDMDHRDYGIGCQILRDLGIRKLKLLTNNPTKRVGLDGYGLEITESRVAEATLPTKYGGEFKIIVYENEVDDMKHIALVKGDIQPEDEVLVRVHSECVTGDLFGSLRCDCGDQLHKAMEMVGQEGKGVIVYMHQEGRGIGLANKIRAYDLQEQGRDTVEANIELGFKEDLRDYGIGAQILVDLGVRKMRMITNNPKKIVGVEGYGLTVVERVPHAEVPSVEE",
    "response#2": "RAVESKLPTAHGEFKLIAYETIVDQQNHMAFVKGDVADGEPVLVRVHSQCATGDTFGSLRCDCGHQLETALRMIEKEGRGVLIYLMQEGRGIGLINKLKAYNLQDEGFDTVEANEKLGFKADLRDYGIGAQILQDLGVRKMRLLTNNPKKIVGLEGYGLEIVERLTAEQEPTVRVDMPTEYGHFELVAFKEKNNKAEHLALIKGDIGNGEPVLTRVHSSCFTGDILGSLRCDCGDQLHAAMQMVEKEGRGLILYMNQEGRGIGLINKLRAYKLQEQGYDTVEANLELGFGVDERDYGVGAQILRYLGATKLRLMSNNPRKRAGLNGYGIEIVETVSPRMPTPFGEFHVYAYQSDITGEEHVALVMGEIADGEAVLVRVHSSCLTGDVFQSARCDCGHQLERAFELIAAEKKGVILYLLQEGRGIGLVNKLKAYELQDQGHDTIEANAKLGFAPDIRDYGTGSQILRDLGVRKIRLMTNNPAKYVAIEGFGLEIVERVRAVESKLPTAHGEFKLIAYETIVDQQNHMAFVKGDVADGEPVLVRVHSQCATGDTFGSLRCDCGHQLETALRMIEKEGRGVLIYLMQEGRGIGLINKLKAYNLQDEGFDTVEANEKLGFKADLRDYGIGAQILQDLGVRKMRLLTNNPKKIVGLEGYGLEIVERLTDQQVRAVESKLPTAHGEFKLIAYETIVDQQNHMAFVKGDVADGEPVLVRVHSQCATGDTFGSLRCDCGHQLETALRMIEKEGRGVLIYLMQEGRGIGLINKLKAYNLQDEGFDTVEANEKLGFKADLRDYGIGAQILQDLGVRKMRLLTNNPKKIVGLEGYGLEIVERL",
    "response#3": "VRVDMPTEYGHFELVAFKEKNNKAEHLALIKGDIGNGEPVLTRVHSSCFTGDILGSLRCDCGDQLHAAMQMVEKEGRGLILYMNQEGRGIGLINKLRAYKLQEQGYDTVEANLELGFGVDERDYGVGAQILRYLGATKLRLMSNNPRKRAGLNGYGIEIVETVMARDDVAIYPFAETSPECPLHKGKAGPLRFVSMKELAEYLLRGGRIQREERPLRFPAPQVGSA"
  },
  {
    "reference": "MRQRTIVCPLIQNEGDYLLCKMADDRGVFPGQWALSGGGVEPGERIEDALRREVREELGKKLVLQQITPWTFSDDVRIKTYADGSKEEIYMIYLIFDCISANREVVINEEFQDYAWVSPADLTKYDLNVATRRTLSLKGLL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should contain at least one Nudix box motif to achieve its intended function.2. The protein must exhibit magnesium ion binding, dCTP diphosphatase activity, dTTP diphosphatase activity.3. The protein have enhanced dUTP + H2O = diphosphate + dUMP + H(+).4. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.5. The protein contains novel Nudix hydrolase domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "GRGVDVGVAVLVQSINKKVLLTRRSKSLNIFPNVWVPPGGHVELGEQLLEAGLRELQEETGLRLQEVSWSMLGLWESAFPPLLSRGLPSRHHIVTYLLVQTNETHQQMQERLCPDEREVSACVWLDTEIAKHIVAAEDSGSPCGPLG",
    "response#2": "MRQRTIVCPLIQNDGAYLICKMADDRGVFPGQWALSGGGVEPGERIEEALRREIREELGDKLVLSQITPWTFRDDTRMKTYPDGTKEQIYMIYLIFDCISANREVSINDEFQDFAWVKPADLHRYDLNDATRRTLTLKRVR",
    "response#3": "MVVKAAGLVIYRKLAGKIEFLLLQASYPPHHWTPPKGHVDPGEDEWQAAIRETKEEANITKEQLTIHEDCHETLFYEAKGKPKSVKYWLAKLNNPDDVQLSHEHQNWKWCELEDAIKIADYAEMGSLLRKFL"
  },
  {
    "reference": "MKVIVVKDQAAGGKEGFKVFKNALDNGAKVFGLATGSTPVTTYKEIVNSDLDFSDCTSVNLDEYVGIAPDNDQSYKYFMQTHLFNDKPFKESFLPNGLASDPEAEVKRYDKVIDEHPIDLQILGIGRNGHIGFNEPGTPRDITTHVVDLTESTIEANARFFASENDVPKQAFSMGLASIMKSKHLLLEAFGENKADAVKGMIEGPDTPELPASILQNHPDVTVIIDEAAASKLSKKY",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor; for enolization step, For ring-opening step to enable effective catalysis of alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-   phosphate + NH4(+).2. The protein must exhibit glucosamine-6-phosphate deaminase activity.3. For general function, the protein need meet that Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion.4. A protein should be able to manipulate the activity of enzymes in the D- fructose 6-phosphate from N-acetylneuraminate: step 5/5, Amino-sugar metabolism, N-acetylneuraminate degradation pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "AAMVASQVILKPDSVLGLATGDTPIGMYKEIINIYKNQKMDFSKARTFNLDEYYGLNRENPQSYYYYMMNNLFNHVNIDKNNINIPNGMADNIEIECKEYERKIDKAGGIDLQILGIGVNGHIGFNEPNISFESETHLVNLNEKTIESNSRFFSSKEEVPTKAISVGIKSIIHSKKIILLACGSAKSDAVSKTINGKINPNIPASILQLHAAMVASQVILKPDSVLGLATGDTPIGMYKEIINIYKNQKMDFSKARTFNLDEYYGLNRENPQSYYYYMMNNLFNHVNIDKNNINIPNGMADNIEIECKEYERKIDKAGGIDLQILGIGVNGHIGFNEPNISFESETHLVNLNEKTIESNSRFFSSKEEVPTKAISVGIKSIIHSKKIILLACGSAKSDAVSKTINGKINPNIPASILQLHNGAQVLGLATGSSPLGLYQKMTSSSADYSNLISINLDEYVGLKPTDPQSYHYFMEHHLFAQKPFAKSFIPDGSNLNATEVINHYNKILATYPIDTQILGIGNNGHIGFNEPGTPFDSQTHKVKLTPATINANARFFTSSKDVPTEAYTMGIGSILQAKHIILLAFGEQKADTINKMVNGKITTAVPASALQQL",
    "response#2": "EMSRRAAEIVKEQILKKPDTVLGLATGSTPLGMYKYLIEMHKNGEIDFSNVITFNLDEYIGLSPDHPQSYHYFMYENLFNHINIKKENIHIPNGVAEDLEEECKRYEREIRRIGRIDLQILGIGVNGHIGFNEPDESIETKTHIVTLTEETINANKRFFKSIEEVPRRAITMGLSSIMKARKIMLLASGSNKAKAIKETLKGRLTTKVPSTVLVPGLEGGMIAGENGAQVLGLATGSSPLGLYQKMTSSSADYSNLISINLDEYVGLKPTDPQSYHYFMEHHLFAQKPFAKSFIPDGSNLNATEVINHYNKILATYPIDTQILGIGNNGHIGFNEPGTPFDSQTHKVKLTPATINANARFFTSSKDVPTEAYTMGIGSILQAKHIILLAFGEQKADTINKMVNGKITTAVPASALQQL",
    "response#3": "EMSRRAAEIVKEQILKKPDTVLGLATGSTPLGMYKYLIEMHKNGEIDFSNVITFNLDEYIGLSPDHPQSYHYFMYENLFNHINIKKENIHIPNGVAEDLEEECKRYEREIRRIGRIDLQILGIGVNGHIGFNEPDESIETKTHIVTLTEETINANKRFFKSIEEVPRRAITMGLSSIMKARKIMLLASGSNKAKAIKETLKGRLTTKVPSTVLNGAQVLGLATGSSPLGLYQKMTSSSADYSNLISINLDEYVGLKPTDPQSYHYFMEHHLFAQKPFAKSFIPDGSNLNATEVINHYNKILATYPIDTQILGIGNNGHIGFNEPGTPFDSQTHKVKLTPATINANARFFTSSKDVPTEAYTMGIGSILQAKHIILLAFGEQKADTINKMVNGKITTAVPASALQQL"
  },
  {
    "reference": "MLLKDILYKVAIRKVYGQTQVPVSDLFIDSRHVQAGGCFIAIRGHQADGHAFINAAIEKGATIIVCEQMPERLTDTVTYVQVEDSANAAGLMAHLFFEEPSLSLKLVGVTGTNGKTTIATLLWKAFTAMGHTCGLISTVENRIGADVLDATHTTPDAISLNRLLRRMKDADCSHVFMECSSHAIHQQRIAGLQFTGAIFSNITHDHLDYHKTFDEYIRVKKSWFDALPATAFALSNADDKRGHVMLQNTAARKCYYSLKTLADVKGRILDNALTGLHLTVNDTEVFVKLIGEFNAYNLLAVYGAAVLLGKDKEEVLKVLSTLTGAEGRFDYIISTREHVVGIIDYAHTPDALLNVLATIKKLRKGPEQIITVVGCGGNRDKAKRPVMAEVACQHSDRIILTSDNPRNEDPAAILEEMQAGVNAACKRKCISIADRKEAIKTAVSLSGKEDIILVAGKGHEKYQEIGGVKYAFDDKKVLEEMFSLMEK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should contain at least one Meso-diaminopimelate recognition motif motif to achieve its intended function.2. The protein must bind to Mg(2+) in order to perform its enzymatic function.3. A protein that can perform UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity in cytoplasm to support cell division.4. The protein should have a high affinity for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP. The designed protein sequence is ",
    "response#1": "MRLTGSTGKSSTKEMLAQVLNTSSPTLKTQANENNMIGLCKTIPRIRPEHRFGVFELGTNAFGEIAALADICNPDAAIIINIGPSHLEKLIDEDGVFREKTALFNRPLEIRLFDGDDPRFSIYADKGKSVGFSDNSDFHITDLECDDTFCSRVCEQLVGITGTNGKTTTSMLLYQALKELGYSCGWIGTLGYYINDEHFITHHTTPDILELNQILARMVEKGIKYVSMEVSSHALALDRVYGIEFDYCLFTNLSREHLDFHQTMEEYGAAKKKLFEPTIQHKAVGLINIDDNFGKTLFEELKSANAYVFSIGTSDADFIIRTEHSHIPHSTTGNRFSLQTPEGIINIRSSLLGSFNMNNLAMCAAYGVLMGMDMKDILEGLSRFPGVPGRMECFGGSDAPLVVVDYAHTPDALEKALVNVREHTSGRLITVFGCGGDRDRGKRPLMGSIAESLSDGLILTDDNPRTESPEAIVADILEGVGERERVRVEHQRGLAIRLAIALAGRGDAVVVAGKGHEDYQEINGERHPFSDAEVVRELIGA",
    "response#2": "MKIITGTQGKTTTAYLLDAALRAGRTSTGLIGTVGFALDGQDLGGSRTTVTTPESPELQGLLGYLLEHGAQALVMEVSSHALALGRVDAIVFDVAAFTGFGTDHLDFHGDVESYFEAKASLFTPERTRHAVLNVDDARGEVLVGRARAAGLGVTTIGAGPYADVHPVRTELAEHGRTRLVARVHGEQVELTLPLPGEYNLRNALTALAITGLSLDSRQVQPGDLFLAMAGELHDGRQFMEQAVAAGAVAVAADAPASGFVEALPVPLIEIPELAQEAGLIAARFFGVDGEHMRGVIEDFGGVAGRMELVPAPESSSGEDLPTVVVDFAHTPDAVASALAALREDAGGALVAVIGAGGSRDPGKRPAMGAAAAAHADLVVVTDDNPRAESPEAIRAALVDGARRGPGEVVEVAGRRAAIAHALRAARPGGTVALLGKGDEVGQTVGELLVSGVSLSSLAVQPGDLYAALPGANAHGAQFVVQAAAAGAAAVLTDERGARLVHESGVDLPTIVVEDPRAILGAVAERLGLPSVALVAVTGTNGKTTTAFLLDAGLRAAGHTTGLIGTVQTRIGGTVVPSVRTTPESSDLQALFATMVERGVTAAVMEVSSHALAQHRVDALRFTGAAFTNLSQDHLDFHPTMEDYFAAKATLFEAGRSKAAVICVDDDWGRRLARQRPDARTYSVTGADADWWPEDVVAGPAGTVCRALGPDGAKADLSVALPGRFNLANALGALATVGLLLGEPFDAVAEGIARLQPVAGRLERVPAAPGRPTVLVDFAHTPDALAKALAAVREHCAGEVWCVFGCGGDRDRGKRATMGTVAVTGADRVIVTDDNPRSEDPQQIIADVLSGIASLGRLQVVPDRADAIARAIRSAGPGDAVLIAGK",
    "response#3": "MHVTGTNGKTTVTRLLAQLLRALKQPSGVIGTLGASLGDELSEARNTTPDALALQQCLAQWRDQNVAAVAMEVSSHALVQGRVNGLHFDTAVFTNLSRDHLDYHLSMAAYARAKATLFAWEGLRHALINSDDPYGATLATSAAANTQVLRYSAAGDPAAELRASNLHFHSGGVKGLISTPWGEGEFSSPLPGDFNVANLLAAIGCAVAQGVDLEAFAAGIAALEPVPGRMELVPAPESSSGEDLPTVVVDFAHTPDAVASALAALREDAGGALVAVIGAGGSRDPGKRPAMGAAAAAHADLVVVTDDNPRAESPEAIRAALVDGARRGPGEVVEVAGRRAAIAHALRAARPGGTVALLGKGHEAYQETAGERVHFDDREQARAALALLAAH"
  },
  {
    "reference": "MKIPFEKETYRFLTDDTHELDGHTLFLKTSQNNQYYEALEEKPSIVTPEELIAFWKIDKLKVVGVTGTNGKTTVTAAIYSFLIDLGERPALQGTRGLFAQEKRIEEKSMTTPSVLETLNNMKKTMDLGCNYFIMEVSSHAIDQKRIEGIKFALKVHTNVTSDHLDYHGTVEEYRRVKSLFFADESPKLLNKDDIKNIAYNPIGAQSYGVDQPATFKVQAFSLLDGITAGIKHLKEEATFHSPMVGLFNLFNLMAAVGSVAMLTGKKVEEICEVVENFAGVAGRMEVVSRDPLIIVDFAHTDDGMYQVLDSIKDRDISVVFGAGGNRDKSKRPRMGAVAGKFAKKIYVTSDNPRDEVPEMILEDILAGLEGKNHVVATPDRKLAIKMALDELKKGDALLILGKGDEDYQEIKGVKYHFDDREVVRALLAHGA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that can perform magnesium ion binding in cytoplasm to support cell wall organization, cell division, cell cycle, peptidoglycan biosynthetic process.2. The designed protein should contain one or more Mur ligase central, Mur ligase C-terminal domains that are essential for its function.3. The designed protein should contain at least one Meso-diaminopimelate recognition motif motif to achieve its intended function.4. The protein should be able to bind ATP, meso-2,6-diaminoheptanedioate, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate ligand in a variety of conditions5. The desired protein should be able to perform ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate6. A protein should be able to manipulate the activity of enzymes in the Cell wall biogenesis, peptidoglycan biosynthesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "MKLRELLKGIECIEVIGENDVAEIKGLTRDSKVIKEGFLFFASKGMSKDGKDFIDEAFKKGAAAAVIDKEEIKGLTRDSKVIKEGFLFFASKGMSKDGKDFIDEAFKKGAAAAVIDKEVPEEITSIENDNRKVRQGGLFVCIKGYTVDGHDFAESAEKNGAAAVLAERELSLNIPVIGIVITGTKGKTTVSYFLEKAFLDFKLKPAVIGTVNYRAMGKIIYPAPNTTPSEPLLSEILRSFLDMGCASCIMETSSHALALKRTENVNFDAALFTNLQSDHMDFHKTRKDYAQAKRKLFKSLSESSKKNKIAVINADDEAADLMASACSEDIKIIFYGTKKEGDIRAENIVEKPEASYFDIKIEGKKTESASLKMIGEHNIYNFLAAASVGYLLDLDFDKIKEGLASMKGVDGRMELFSYVKKPTFIIDYAHTPDALKKTLNAIHSHYQRYIWCIFGCGGERDQKKRPIMGAIAEKMSDKVIITNDNPRNEKEKKIIQDILNGCKNKEKILIIPDRKKAISYAYFGAKYHHIILIAGKGDEDYQEINGVKYHFSDREVVREILKERG",
    "response#2": "MNITGTNGKTSVSHYLAGMLEGLQQKAAVIGTVGIGAPGALRTATHTTPDLVQLHKVMSELKQEGYTYQAMEVSSHALDQLRTAGVPFEVAVFTNLSRDHLDYHGTMAAYGAAKSHLFIRYPIHYAVINADDAYSTTLEQSINQSDHHPRVYRYSLNDSKADLYCVSCRAEPSGFALSLAGRWGAFTVQLPLLGRFNVANALAAATVALAXGAQADDIAAGLAAFDGVGGRLEHVHDPAGRITALVDYAHTPDALRNVLETLRALLPDGHRLSCVVGAGGDRDRSKRPEMARIAAELADQVILTTDNPRSESPEAILDEMEAGVPPAAAPRVLRITDRRQAIRTAVRLGHEVLLVAGKGHETGQIVGATTLPFDDVAVAREAVARL",
    "response#3": "MKLRDLVPGAAVDEIAGSVDVSIGQLTLDSRQAGPGSLFVALRGTQTDGHRYIQTAVELGARTILCEELPALTRPDVTYVQVLDSARALGLLASAFYDHPSARLRMIGVTGTNGKTTTTYLIERILSDAGQRAGLVGTVRIRIGDREVDAPRTTPDAVELQRLLAEMRDAGCDHLVMEVSSHALALDRVRGCEFAVAVFTNLSQDHLDFHGSMEAYFAAKARLFTPDHGRLQRIATPNGFSVIVDYAHTPDAMEKALSCVREWTKGRLIVVFGCGGDRDRTKRPLMAQVASKFADLIVITSDNPRTEAPERIIEDIIAGMPVTWQKFSADESHFSAPKSKSYTIEVDRRQAIHIAINVARPGDTVFIGGKGDEGYRVTVGAGAEPPLTVPLVGRHNVENILAAAAAALAAGVELAGIAAGLAAAPPVP"
  },
  {
    "reference": "MKIEINENFCKGCDICIVVCPRGVFEKSKKLNKKGIYPPIPVNPEKCTKCNLCILQCPDQAISIELSQE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should incorporate a binding pocket that specifically recognizes [4Fe-4S] cluster to enhance enzymatic efficiency.2. The protein must exhibit oxidoreductase activity. The designed protein sequence is ",
    "response#1": "MTLCVSTQVGCAIGCKFCASGLDGLKRNLSTGEIVGQILLARNQAKRRIDNLVFMGMGEPLANLPALLPALDQILSPKGLGIGARHLTLSTSGLAPKIIELAKYPAQIRLAISLHGGDDETRQKIMPINQKFPLEQLLQACEKFIQERKKMITLEYILIEGVNDDLTQAELLVAHAKRLRAKINLIPYNRVDGLEWKRPSVEKIRAFHRR",
    "response#2": "MTLCVSTQVGCAIGCKFCASGLDGLKRNLSTGEIVGQILLARNQAKRRIDNLVFMGMGEPLANLPALLPALDQILSPKGLGIGARHLTLSTSGLAPKIIELAKYPAQIRLAISLHGGDDETRQKIMPINQKFPLEQLLQACEKFIQERKKMITLEYILIEGVNDDLTQAELLVAHAKRLRAKINLIPYNRVDGLEWKRPSVEKIRAFHRS",
    "response#3": "ISTQVGCRMGCSFCASTKEGLVRNLNPAEMANQIYNIEEDLNCKINNIVLMGSGEPLDNYDNVIKFLKIIHHEKGKNISYRNITLSTCGIVPKIYALADEGIPINLSISLHSPFNHIRERIMPIGKKYLVEEIIKACKYYYNKTNRRITMEYTVIKGINDREKDLEER"
  },
  {
    "reference": "MKAFTQHHGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDVGQPYQDNSKRPLNPDFVLNHERYQGASVLLARENFGCGSSREHAPWALEEYGFCAIIAPSYADIFFNNSFKNGLLPIILSEEDVDQLFKQVEASPGYQLSIDLQAQTVTRPDGKVLSFEIDAFRKHCLLNGLDDIGLTLMDAEAIAGFESRHRASQPWLFRD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should take into account the physiological conditions of the L-leucine from 3-methyl-2-oxobutanoate: step 2/4 pathway. The designed protein sequence is ",
    "response#1": "MTMIQALRSAMDIMLERDDDVVVFGQDVGYFGGVFRCTEGLQKKYGTSRVFDAPISESGIIGAAVGMGAYGLRPVVEIQFADYVYPASDQLISEAARLRYRSAGDFIVPMTVRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNPYDAKGLLIACIENDDPVIFLEPKRLYNN",
    "response#2": "SMTMIQALRSAMDIMLERDDDVVVFGQDVGYFGGVFRCTEGLQKKYGTSRVFDAPISESGIIGAAVGMGAYGLRPVVEIQFADYVYPASDQLISEAARLRYRSAGDFIVPMTVRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVMPSNPYDAKGLLIACIENDDPVIFLEPKRLYE",
    "response#3": "MDRSNVDTDMIIPKQFLKSIKRTGFGPNLFDELRYLDEGKPDQDCSNRPLNPDFVLNQDRYKNASVLLARTNFGCGSSREHAPWALDDFGFRVIIAPSFADIFYNNCFKNGLLPIVLDEKVVDRLFRETEENEGYQLTVDA"
  },
  {
    "reference": "MKVLILAAGLGKRMKSKYPKVVHKILGKPMINWVVDLAKNFGEVGVVVGHKAEVVKGYLSDDVKTYLQEPQLGTGHAVMCAKEFISENEDLLVLYGDVPLLSKETIEMLRNEHEEKNNQVTVLTFIAENPAGYGRIVRTDGKIRIVEDKDASEEEKKINEVNSGIYIFSGKFLLENIENLKNNNAQGEYYLTDLVGMAERVSTVLLEDILEVSGVNDRVQLAELESIARERILKKLMLSGVTIVDPVSTFIGPDVEIGMDTIVHPFTIIEGKTKIGEDCEIGPYTHIADSTIGKNVKVIRSEVEKSIIEDDVSVGPFSRLREGTVLKEKVKIGNFVETKKSIIGKNSKAQHLTYLGDATVGENVNVGAGTITCNYDGYKKYKTFIGDGAFIGSNSSLVAPVKIGKGAITGAGSVITEDVPDDALALGRARQVIKEGWAMKKREEMKNADNKE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein with the reaction acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate, the Proton acceptor must be optimized to ensure efficient catalysis.2. The protein should be able to bind Mg(2+), acetyl-CoA ligand in a variety of conditions3. The protein should be optimized for activity in Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The protein must exhibit magnesium ion binding. The designed protein sequence is ",
    "response#1": "ALVLAAGKGTRMKSGFVKVLHEAAGAPLIDWPVAAAREAGAGRVVLVAGHQADRVQAHFSTDPAVSFALQQEQLGTGHAVACAAGALEGFSGTILILCGDVPLIRPETLQGMLAAHRDLGAALTVLTTHVDNPFGYGRVVKGEGGRILRIVEQKDATPEELKINEINSGIYCVESEFLFAAVASLSNDNAQKEYYLTDIVLRAAEQELLALAFEDSSEAQGVNDRTQLDLAEAEAAFQARRRQEMMESGVTLIAPDTVFFSHDTRIAADVVVEPNVVFGPGVNVASNVRIKAFSHLEGASLGEGAEAGPFARLRPGAELGANAKVGNFVEVKKARLGEGAKASHLTYLGDTDVGAHANIGAGTITCNYDGFFKHRTVIGEGAFIGSNTSLVAPVTIGAGAMTGSGSVITEDVPDNALALSRARQVNKEGWRLRKQSDLPKVLHKIAHAPMLWHALRAGLVLEPEKTVVVAGHGADLVQASAHEFDPEIQIALQTEQLGTAHAVAQARPALDGFEGDAIVLYGDTPFIREETLAAITQARVSNDVVVLGVEHPQELF",
    "response#2": "ALVLAAGKGTRMKSGFVKVLHEAAGAPLIDWPVAAAREAGAGRVVLVAGHQADRVQAHFSTDPAVSFALQQEQLGTGHAVACAAGALEGFSGTILILCGDVPLIRPETLQGMLAAHRDLGAALTVLTTHVDNPFGYGRVVKGEGGRILRIVEQKDATPEELKINEINSGIYCVESEFLFAAVASLSNDNAQKEYYLTDIVLRAAEQELLALAFLSRGQILAHEFDDVEGANDRAQLAELDLAEAEAAFQARRRQEMMESGVTLIAPDTVFFSHDTRIAADVVVEPNVVFGPGVNVASNVRIKAFSHLEGASLGEGAEAGPFARLRPGAELGANAKVGNFVEVKKARLGEGAKASHLTYLGDTDVGAHANIGAGTITCNYDGFFKHRTVIGEGAFIGSNTSLVAPVTIGAGAMTGSGSVITEDVPDNALALSRARQVNKEGWVAKRRKS",
    "response#3": "ALVLAAGKGTRMKSGFVKVLHEAAGAPLIDWPVAAAREAGAGRVVLVAGHQADRVQAHFSTDPAVSFALQQEQLGTGHAVACAAGALEGFSGTILILCGDVPLIRPETLQGMLAAHRDLGAALTVLTTHVDNPFGYGRVVKGEGGRILRIVEQKDATPEELKINEINSGIYCVESEFLFAAVASLSNDNAQKEYYLTDIVLRAAEQELLALAFQQHQVLVNAAIVPDLAEAEAAFQARRRQEMMESGVTLIAPDTVFFSHDTRIAADVVVEPNVVFGPGVNVASNVRIKAFSHLEGASLGEGAEAGPFARLRPGAELGANAKVGNFVEVKKARLGEGAKASHLTYLGDTDVGAHANIGAGTITCNYDGFFKHRTVIGEGAFIGSNTSLVAPVTIGAGAMTGSGSVITEDVPDNALALSRARQHIDGWVARRQAQQQAKES"
  },
  {
    "reference": "MKKRARKLRLPSTTDPNLRWALAYLLATVLLLLLWQEVAGDWRVRTIPYSQFKAHLARREVVQAAVKPDEITGRIVPRPDVKTNAPPAATESPGADAVPAGVRRAQAETKPFLFRTVRVEDADLVKELQAAGVEYTGTRPSALAQFLLAWVLPIVLMVGLWSLIARRFSAAREGLLGFGRSRAKLIVDKNTGVTFADVAGCEEAKQELREIVDFLKNPRDYQALGARIPKGALLVGPPGTGKTLLARAVAGEAGVPFFSMSGSDFVEMFVGVGAARVRDLFQQAKQHAPCIIFIDELDAIGRQRGVHVGTVNDEREQTLNALLVEMDGFEANTGVILLAATNRPEVLDRALLRPGRFDRQIVVDAPDLDGREAILKVHARNKKLAPEVDLRRVAAATAGFSGADLANVLNEAALLAARRKSKLITQRDLEEAIEKVVAGPERKSRRLSPEDKRRVAYHEVGHALVAAYSEHADPVHKISIVPRGRAALGYTMQLPAEDQFLLTRSELLDRIRGLLGGRAAEEIVFGEVSTGAQNDLERATALARQMVAVYGMSERIGLAHCAAHPPMFLAGSDTPLQRDCSEQTAREIDEEVKAILERAYTEAKQCLIAHRDQLEQVTAELLAKETLDGAEFYRLVGRPMPCAKEPVPPLPAPGAVAAAQGRSSSSSQPAP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have zinc ion binding, metalloendopeptidase activity, ATP hydrolysis activity, ATP-dependent peptidase activity within plasma membrane to regulate protein catabolic process, proteolysis.2. The protein must exhibit the following characteristics: Plays a role in the quality control of integral membrane proteins.; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins.3. The protein should have a high affinity for ATP, Zn(2+).4. The protein contains novel AAA+ ATPase domains that confer a unique function or activity.5. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.6. The protein must bind to Zn(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MVMSEEEKKLTAYHEGGHALVALHMEDSDPVHKATIIPRGRALGMVMRLPERDQLSVTRSKLKADLAVAMGGRVAEEVIFGHDKVTSGASSDISMATKMAKAMVTQWGMSDKLGPLAYQDNEEEVFLGHSVARQQTMSEDTARMIDEEVKRIVSEGYEKAQELLTKHLDELHTLAQGLLEYETLSGEEIKARYDRLKAKGLI",
    "response#2": "VLLVGPPGTGKTLLAKAVAGEANVPFFSMSGSDFVEMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAIGRARGRNVNFSANDERENTLNQLLTEMDGFASNQGVIILAATNRADILDRALMRAGRFDRQIHVLFYPVDLHTYARVQLFLEKQGIPFLPQNVEVKTLARGTPGFSGADLANLVNEAALLAARRGRRLVTMAEFQRAVKIPKGVLLVGPPGTGKTLLARAIAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLNQLLVEMDGFEPNESIILIAATNRPDVLDPALLRPGRFDRQVVVPNPEGVMEFNVDLRIIARGTPGFSGADLANLVNEAALTAARIGRRFVTMEDFNKAIERGMAETHFGQRKGLF",
    "response#3": "MVMSEEEKLKTAYHESGHAIVGRSLEEHDPVYKVTIIPRGRALGVTMFLPEEDKYSYSHQSILDRICGLYGGRIAEELIYGEKGVTTGASNDIERATELAHNMVTKWGLSEKLGPIKYGDETDEPFLGRSAGASKQGISDDTAHEIDNEVKTILSNCYQHAKDILSENIEALHAMSKALMDYETLDVDQIDDYVRRFEGLG"
  },
  {
    "reference": "MSGSDGGGLMSSAGLVRYFDAEDQNTIRIDPRTIVATGVMFGLLMLVLNAMIV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should be able to enhance the efficiency of protein transport.2. For general function, the protein need meet that Involved in protein export; The function of the beta subunit is unknown, but it may be involved in stabilization of the trimeric complex.3. The designed protein should contain Extracellular, Cytoplasmic topological domains.4. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "LDAQGAKIFGDFSGANVGKRMAIVLDNKVYSAPVIRERIGGGSGQISGNFSVAQASDLAIALRSGAMSAPIQVLEKRIIGPSLGKDSVKTSIIALVGGFILVMGFMVLYYSMAGVIACLALVVNLFLIVAVMAIFGATLTLPGMAGIVLTVGIAVDANIIINERIREVLRENEGIAKAIHLGYINASRAIFDSNITSLIASVLLYAYGTGAIKGFALTTGIGILASIITALFSNYFFN",
    "response#2": "DQDNSGFYTFFNQTFFPIDNMGYDVDPSFRIYKQSGNYHNFHFCVKMNSRFTYNGGEVFNFKGDDDLWVYIDDKLVVDVGGIHPAQDGSVNLDTLGLIKNKVYPLDLFYCERHTDGSTISIQTTIQAYCAWYDYCGVCGGS",
    "response#3": "DQDNSGFYTFFNQTFFPIDNMGYDVDPSFRIYKQSGNYHNFHFCVKMNSRFTYNGGEVFNFKGDDDLWVYIDDKLVVDVGGIHPAQDGSVNLDTLGLIKNKVYPLDLFYCERHTDGSTISIQTTIQAYCAWYDYCGVCGGD"
  },
  {
    "reference": "MNIFTTSILLIFILLLSPILISMSNLIKHINFPLYTTTSIKFSFIISLLPLLMFFHNNMEYMITTWHWVTMNSMELKMSFKTDFFSILFTSVALFVTWSIMQFSSWYMHSDPNINRFIKYLTLFLITMLILTSANNMFQLFIGWEGVGIMSFLLIGWWYGRTDANTAALQAILYNRIGDIGFILAMVWFSLNMNSWELQQIMFSNNNDNLIPLMGLLIAATGKSAQFGLHPWLPSAMEGPTPVSALLHSSTMVVAGIFLLVRFHPLTTNNNFILTTMLCLGALTTLFTAICALTQNDIKKIIAFSTSSQLGLMMVTLGMNQPHLAFLHICTHAFFKAMLFMCSGSIIHSLADEQDIRKMGNITKIMPFTSSCLVIGSLALTGMPFLTGFYSKDLIIEAINTCNTNAWALLITLIATSMTAMYSMRIIYFVTMTKPRFPPLISINENDPDLMNPIKRLAFGSIFAGFVISYNIPPTSIPVLTMPWFLKTTALIISVLGFLIALELNNLTMKLSMNKANPYSSFSTLLGFFPSIIHRITPMKSLNLSLKTSLTLLDLIWLEKTIPKSTSTLHTNMTTLTTNQKGLIKLYFMSFLINIILIIILYSINLE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be able to modulate mitochondrial electron transport, NADH to ubiquinone in a way that leads to a desirable outcome.2. For general function, the protein need meet that Essential for the catalytic activity and assembly of complex I. The designed protein sequence is ",
    "response#1": "RASIESNFALRNLVGADNFYSGMSFFEQERLSLIFEILRNSGIYIPSVCDIENYDAILILGEDVTQTSARIALAIRQAVRSKSDHMAKYVGIYKWQSLAVTNFAQDAKYPLFITGIDKTKLDDIAMYTYYDSVQNQARFGFSIAYAVDNQVSFVKDFNLNGLDITKLDLVVEKLMCARKPLIVSGSNSGSKELIIAAVNIAYALKRKKSDVGLVLVDHDVNSIGLSMMTSKSVDDVFNTLSDTNNGNAVFNTVIILENDLYRHASVAKINKMLSNINYLIVLDHQYNIIQDKADLIFPVSSFAESDGTVINYEGRAQRFFKLYNPQCPVCEEGGNCHLYA",
    "response#2": "SSNWIIIWIGLEISLLGFVSYYMLMKKIMSGEGIMMYFLIQSVSSTVMLLNGLYIFVNHASSYIYLFIFITMLMLKIGMFPLHFWIIPVYSKLSYLNIGIVGLLLKIVPMWILMHMGCITSEMLNLITMLSVTSMLFGALIGMNLSKMRMVLGASTITHNGWLGMSCISGSLFKYFITYGFSLVILLF",
    "response#3": "YSTRIMFFVLLGQPRFNTLNLINENNTHLINSIKRLLIGSIFAGYLISYNIPPTTIPQMTMPYYLKLTALAVTIAGFILALELNLAAKNLKFMYPSNLFKFSNLLGYFPIVMHRLPSKMSLTMSQKSASMLLDMIWLENVLPKSISLFQMKMSTTVSNQKGLVKLYFLSFMITLALSLIF"
  },
  {
    "reference": "MSLSIIILAAGKGTRMKSAKPKVMHTLANKPLLQHVIDTAKQLNPSQLAVVCGSGADDVVPYLQQQGIDTAMQLEQKGTGHAVEQAKAFYQESDQVLVLYGDVPLIEKDTLEALIHEGDANTLKVLTTVLDDPTGYGRIVRDVTGRMLMITEEKDANEETREINEVNTGIMCIPAKWLNSALSQLDNNNAQGEYYLTDLIAKAVEEGIEIDSITCEDEQEVAGVNNRVQLAELESYYQQKKATELMMAGVTMRDPARVDIRGELTVGQDITVDVNVIFEGNNTLANDVTIGANCIIINSIIHEGAEILPNSIIENAEVGTNCSVGPYARLRPGSRLAAKAKVGNFVEIKNANIGLGSKINHLSYIGDTDMGADVNIGAGTITCNYDGANKHRTVIGDRVFVGSDTQLVAPVVVEDGATIGAGSTIRKDAPAGELTLTVSKQKTVSGWQRPVKKKS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for acetyl-CoA binding. The designed protein sequence is ",
    "response#1": "GFDKFYENWDTYSKKLCLRSQVVSNSPEIIRRLGTIAMNTPVEVDIYAHANSTNVMGSRMLNGLGGSADFLRNSKLSVMHTPSARPSKTDKTGVSCIVPFATHVDQTEHDLDIVVTDQGLADLRGLSPKERAREIITKTSHPDYRDQLTDA",
    "response#2": "GFDKFYENWDTYSKKLCLRSQVVSNSPEIIRRLGTIAMNTPVEVDIYAHANSTNVMGSRMLNGLGGSADFLRNSKLSVMHTPSARPSKTDKTGVSCIVPFATHVDQTEHDLDIVVTDQGLADLRGLSPKERAREIITKTSHPDYRDQLTDL",
    "response#3": "MGTSVQSIELVLPPHANHHGNTFGGQIMAWMETVATISASRLCHGHPFLKSVDMFKFRGPSTVGDRLVFSAIVNNTFQNSVEVGVRVEAFDCQEWAEGQGRHINSAFLIYNAVDDN"
  },
  {
    "reference": "MDLLRLSRLFSGPRPIGLSVLQHLDLVGSTRWTGGREGPARLRAAFCGSSSPLPLGSGNQKEMSSLCSDSSKLSTVAPQEEAEEESFGSLSGKFSSRRIFHKSTAQLYNLQLKEQGGEEEELEPRPWRGRRNTQYWYFFQCKRLIKEGKLAEALDLFERQMLKEERLQPLECNYTVLIGGCGRVGYLKKAFRLFNDMKKRDLEPSDATYTALFNVCAESPWKDSALQSALKLRQQLQARNFQLNLKTYHALLKVAAKCADLRLCLDVFKEIIQRGHAVTEETFCFLLVGCIQDKKTGFRQAMQVWRQMLSLGIKPSRHGYNLLLEAARDCGLGDPEVASRLLLTSQEETILLPPPKGRHMAGRKVQAKTVHGVSLRHVEALERQLFLEPSQKLEGPPALPEARVTSRTQPEVETTAEPGHTVALTPLASKPTHLELEVSLLSLGTLSPAVVSFGTVATPADRLALMGGLEGFLGKMTEHGLQPDIKTLTLLAEVVEPGSTAESSLLSVLDRHRVEADVTFFNTLIRKKSKLGDLEGAKALLPILAKKGIVPNLRTFCNLAIGCHRPRDGMQLLADMKKSQVSPNIHIYSTLINAALKKLDYTYLISILKDMRQNSVPVNEVVVRQLEFAAEYPPTFDRYKGKNTYLEKIDGFRAYYKQWLKAMPAEEAPHPWQEFQNKPVGDQDTTDKAGGLRDG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should be able to enhance the efficiency of tRNA 3'-end processing. The designed protein sequence is ",
    "response#1": "QLRPFSVTWNPMGFALSSLVVHTGRTAVLCSVCLEGKVPRWRKGEGLGWLSAEYRLLPGSTPQRQSRELMKLSGRTQEIQRLIGRSLRAVIDMERLGERTLLIDCDVIQADAGTRTASITGAWLALLG",
    "response#2": "QLRPFSVTWNPMGFALSSLVVHTGRTAVLCSVCLEGKVPRWRKGEGLGWLSAEYRLLPGSTPQRQSRELMKLSGRTQEIQRLIGRSLRAVIDMERLGERTLLIDCDVIQADAGTRTASITGAWLALL",
    "response#3": "QLRPFSVTWNPMGFALSSLVVHTGRTAVLCSVCLEGKVPRWRKGEGLGWLSAEYRLLPGSTPQRQSRELMKLSGRTQEIQRLIGRSLRAVIDMERLGERTLLIDCDVIQADAGTRTASITGAWLALLG"
  },
  {
    "reference": "MASERQKKIRNFCIIAHIDHGKSTLADRLLEMTGVLTEREMEDQVLDTMEIERERGITIKAQAVRMVYKAKDGEEYILNLIDTPGHVDFNYEVSRSLAACEGAILVVDAAQGIEAQTLANVYLALDHDLEILPVINKIDLPSAQPDVVKKEIEDVIGLDASEAPLISAKNGINIEAVLESVVKNVPPPEGDENAPLRALIFDSYYDSYKGVIVYIRVKEGTLKLGDKVRMMYTNKEFTVTEIGYMKPGGLVPGTQLSAGEVGYFAASIKNVKDTRVGDTVTTADNPAKEPLPGYKKVNPMVFCGIYPADGSKYGDLRDALEKLQLNDASLTFEPESSVALGFGFRCGFLGLLHMEIIQERLEREFDFDLVTTAPSVIYKVTKTNGETLYIDNPTNLPPPAEIKTMEEPIVKATIMTPTEYVGNIMELAQERRGIYKDMTYIDEGRVMLTYELPLNEIIYDFFDALKSRTKGYASLDYEMLGYRESDLVKLDIMLNGEIVDALSFIVHREKAYARGRRIAEKLKEAIPRQQFEVPIQACIGGKIIARETVKAYRKDVLAKCYGGDITRKKKLLEKQKEGKKRMRQIGTVEVPQEAFMSVLKLDT",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Back-translocation proceeds from a post-translocation (POST) complex to a pre- translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly; May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes; Required for accurate and efficient protein synthesis under certain stress conditions; Binds to ribosomes in a GTP- dependent manner.2. The designed protein should contain one or more tr-type G domains that are essential for its function.3. The designed protein should have ribosome binding within plasma membrane to regulate positive regulation of translation.4. The protein should have a high affinity for GTP.5. The protein should have a low Km value for GTP + H2O = GDP + H(+) + phosphate. The designed protein sequence is ",
    "response#1": "NFSIIAHIDHGKSTLSDRIIQICGGLTERELKEQVLDSMDLERERGITIKAQSVTLDYKAEDGQVYQLNFIDTPGHVDFSYEVSRSLYACEGALLVVDAGQGVEAQSVANCYTAVEQGLEVLPVLNKMDLPQADPDRVAHEVEEIIGIDASDACRVSAKSGLGMEAHHIRNFCIIAHIDHGKSTLADRLLEITNTLDRSQMSSAQVLDDMDLERERGITIKSHAVQMKYRASDGLDYTLNLIDTPGHVDFSYEVSRSLAACEGALLVVDATQGVEAQTIANLYLALDAGLEIIPVINKIDLPSSDVEGVARQIIDLIGIKRDEILQVSAKAGIGVYELIEAIVKRVPAPTGNPDKPLRALIIDSWFDNYLGVVSLVRIKNGVLKKGDKFKVMSTGQAYNADRVGIFTPKMKDQAELKTGQVGYVIAGIKEIHGAPVGDTLTKIEEPWIKATIMVPDDYLGAILTLCSERRGQQVDLTYVGNRAMAVYRLPLNEVVFDFYDRLKSVSRGYASFDYQMDGYEESDLVRIMEEPIVKTTIMVPNTYVGAVMELCQDRRGEMKDMQYIDDTRVNLYYEMPLNEVIYDFFDALKSKTKGYGSLDYELLGYRASDLVKLDIEEPYVKATIMVPNDYVGPVMELCQGKRGTFVDMQYLDEKRVMLIYDIPLSEIVYDFFDALKSNTKGYASFDYELIGYRPSNLVKMDILLNGEKIDALAIIVHKDFAYSRGRDLTVKLKEVIPRQNFEIPVQAAIGAKVIARETIKAYRKDVTAKLYGGDVTRRQKLLKKQKAGKKRMKSIGSVEIPQEAFLAIKN",
    "response#2": "NFSIIAHIDHGKSTLSDRIIQICGGLTERELKEQVLDSMDLERERGITIKAQSVTLDYKAEDGQVYQLNFIDTPGHVDFSYEVSRSLYACEGALLVVDAGQGVEAQSVANCYTAVEQGLEVLPVLNKMDLPQADPDRVAHEVEEIIGIDASDACRVSAKSGLGMEAKIRNFSIIAHIDHGKSTLADRILELTETVEKRDLKAQHLDSMDLEKERGITIKLNAVQIKYKDYVFHLIDTPGHVDFTYEVSRSLAASEGAILLVDATQGIEAQTLANVYLAMDNGLEIIPVINKIDLPSSDPEAVKKEIEEVIGIDASDAILISAKTGKNIEDVLKIRNFSIIAHIDHGKSTLADRLIEYTGLISQREMQSQMLDNMDLERERGITIKLQTIRLVYKAKDGEEYYLNLIDTPGHVDFTYEVSRSLAACEGAVLIVDAAQGIEAQTLANVYLALEQDLEIIPVINKIDLPSARPDEIKTEIEDIIGLDASEAPLISAKEGLNIEDVLIRNFSIIAHIDHGKSTLADRLIEYTGLISQREMQSQMLDNMDLERERGITIKLQTIRLVYKAKDGEEYYLNLIDTPGHVDFTYEVSRSLAACEGAVLIVDAAQGIEAQTLANVYLALEQDLEIIPVINKIDLPSARPDEIKTEIEDIIGLDASEAPLISAKEGLNIEDVLIRNFCIIAHIDHGKSTLADRMLQLTGVVEQRQMRAQYLDRMDIERERGITIKSQAVRLPWAPSHDKGATHILNMIDTPGHVDFTYEVSRSLAACEGTILLVDAAQGIEAQTLANLYLAMENDLTIIPVLNKIDLPAAQPEKFAEELANLVGCDPDDVLKVSAKTGLGVDVLLDKVVAEVRRVRNFCIVAHIDHGKSTLADRLIERTRAVEERLQHAQMTDNMELERERGITIKSHAVCIPYTDAHGTEYVLNFVDTPGHADFAYEVSRAIAACEGALLVVDATQGVESQTISNLYLVLEHNLEIIPVINKIDLPTADVPRVLQQVEHDLGLDPASSVLISAKTGENVDALFDAIITRIPPP",
    "response#3": "IRNFCIIAHIDHGKSTLADRMLQLTGVVEQRQMRAQYLDRMDIERERGITIKSQAVRLPWAPSHDKGATHILNMIDTPGHVDFTYEVSRSLAACEGTILLVDAAQGIEAQTLANLYLAMENDLTIIPVLNKIDLPAAQPEKFAEELANLVGCDPDDVLKVSAKTGLGVDVLLDKVVAEVRNFSIIAHIDHGKSTLADRMLQDTQTVAQRDMKEQFLDNMELERERGITIKLQAARMRYVAKDGEEYILNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQGVEAQTLANVYLALDNNLEIIPVLNKIDLPGAEPDRVTEEIEEVVGLDCTDIIRASAKQGLGINDILIRNFSIIAHIDHGKSTLADRLIEYTGLISQREMQSQMLDNMDLERERGITIKLQTIRLVYKAKDGEEYYLNLIDTPGHVDFTYEVSRSLAACEGAVLIVDAAQGIEAQTLANVYLALEQDLEIIPVINKIDLPSARPDEIKTEIEDIIGLDASEAPLISAKEGLNIEDVLDALRLNGEKIDALAIIVHKDFAYSRGRDLTVKLKEVIPRQNFEIPVQAAIGAKVIARETIKAYRKDVTAKLYGGDVTRRQKLLKKQKAGKKRMKSIGSVEIPQEAFLAIK"
  },
  {
    "reference": "MADGDARIVVATHNEGKLAEMRSILDACIHGLPGEQAARMERFTLVSAGQLGLPDPVETGLTFEQNALLKAGDVTERTGLPAIADDSGLVVDVMGAAPGILSARWSGVHGDDRANMRLLLAQLKDLSEDDLGARFVTAAALVLPAGMGKEEVADRKGPTEFIRTGRMDGHLVHRPRGDQGFGYDPIFVPDDQPERARRAGVELTSAELEPAEKNAISHRGKALKAIMPILIRALS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.2. The Mg(2+), substrate binding site should be located in a region of the protein that is accessible to the ligand.3. The protein must be able to nucleotide binding, ribonucleoside triphosphate phosphatase activity, ITP diphosphatase activity.4. The protein should be designed to have high stability and activity towards dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MKPAKIVLLSGTDTEVGKTLVSCALLRAA",
    "response#2": "MNPAEFDSIDKAFA",
    "response#3": "MIEFVIGSRNKKKIGEIRDILDGTPGVDIRGLDDFPDFPEVEEDGNTFEENARKKAEIFAEHTGLTTLADDSGLEVDALNGEPGVYSARYAGEHGNDAANNEKLLHEMRHVPDEERTARFRCVIAIAMPDGETVTGTGTMRGKILREMRGENGFGYDPLFIPDGYTETFAELGSDVKNTISHRAKALRKMKAALESL"
  },
  {
    "reference": "MRNSLEKVDIRSLSLDQLKVQLASLGEPSFRAGQIYDWLWAKAATCFDEMSNLSKALREQLKSVFVIRAVKVQQSQWSADKTIKSIFKLDDGNSIEGVLIPTPDRMTACVSSQVGCSLTCKFCATGYMDRKRNLNPDEIFDQVVLIDRQAKENYQTPLTNIVYMGMGEPLLNYANVLKSIERITAANGLNMAAKRITVSTAGIAKMIKKLGDDQVRFNLALSLHAANDQKRNEIMPINEQNSLKALADALKYYFANTKNPVTYEYIVFDGFNDSLQDAAELAKFCKHLPCKVNLIEYNPISFAAFQNAAASKIDAFADYLRGQGIVTNIRRSRGKDIDAACGQLAIKTGKEQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be involved in rRNA base methylation by localizing to cytoplasm and exhibiting rRNA binding, metal ion binding, tRNA (adenine-C2-)-methyltransferase activity, rRNA (adenine-C2-)-methyltransferase activity.2. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity.3. The designed protein should have a high specificity for S-adenosyl-L-methionine, [4Fe-4S] cluster binding, with minimal off-target effects.4. The designed protein is the optimization of the Proton acceptor, S-methylcysteine intermediate to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.5. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.. The designed protein sequence is ",
    "response#1": "MTACISSQVGCSLTCKFCGTGRLKLMRNIKFDEIYDQVAIIKEQAEKYYQRPLSNIVYMGMGEPLLNYSQVLKSIEMICGEDGLGMSPKRITVSTAGIAKMIKKLGDDEVKFNLALSLHAANDEKRSKIMPINDQNNLEILAEALRYFYEKTGSRVTFEYIIFKDFNDQIEDARELAEFCKNVPCKINIIEYNPIDNGEFQQADVLKVDDFAAYLEEQHAHSLPRPVESDRGAVCLSSQVGCTLNCKFCYTGTQKLVRNLDSAEIVGQFMAARDSYGEWPSPKKERLLSNIVMMGMGEPLYNYENVARALQIITDKEGISLSKRRITLSTAGVVPLIERCGIELGVGLAISLHAVTDELRNTLVPLNKKYPISELLSACRKYPSASNARRITFEYVMLKGINDSPGDARELLRLLKGIHAKINLIPFNPWPNSPFECSGKGVIKEFSRILNEGGYSAPIRTPRGIEIGAVSGKYPGRSTVCVSSQAGCGMACPFCATGQGGLQRNMSTAEIVEQVVAAARTLARGEVPGGPGRVNNVVFMGMGEPLANYKAVLGAVRRLTDPSPAGLGMSARGVTVSTVGLVPRIRQLTEEGVPVTLALSLHAPDDELRNELVPINTRFSVAETVEAAWDYARVTKRRVSIEYAMMRGINDQAWRADLLGDLLNGYGDWGWVHVNLIPLNPTPGSKWTASDPADEREFVRRLEAKGVPTTVRDTRGQDIMAACGQLKSATERARKSA",
    "response#2": "MTACISSQVGCSLTCKFCGTGRLKLMRNIKFDEIYDQVAIIKEQAEKYYQRPLSNIVYMGMGEPLLNYSQVLKSIEMICGEDGLGMSPKRITVSTAGIAKMIKKLGDDEVKFNLALSLHAANDEKRSKIMPINDQNNLEILAEALRYFYEKTGSRVTFEYIIFKDFNDQIEDARELAEFCKNVPCKINIIEYNPIDNGEFQQADVLKVDDFAAYLEESRHDEPRPGEPYPGRSTVCVSSQAGCGMACPFCATGQGGLQRNMSTAEIVEQVVAAARTLARGEVPGGPGRVNNVVFMGMGEPLANYKAVLGAVRRLTDPSPAGLGMSARGVTVSTVGLVPRIRQLTEEGVPVTLALSLHAPDDELRNELVPINTRFSVAETVEAAWDYARVTKRRVSIEYAMMRGINDQAWRADLLGDLLNGYGDWGWVHVNLIPLNPTPGSKWTASDPADEREFVRRLEAKGVPTTVRDTRGQDVSAACGQLA",
    "response#3": "QEGRIALVGLGREGLASLCAGLGLKPFQVSEVYRWIYGRRVLDPLAWTNIPKAARAALAARTSAQLPEVAGEQLSADGTRKFLLRLSDGQAIEAVYIPDGNRRTVCVSSQVGCAVGCAFCLTARMGFRRNLTPGEILGQFFVLEERTDIRKVGYNVVFMGMGEPMANRANLEEALAILEDEEGMGISWRRITVSTSGLADQLEAFAKSAVCPRLSLSLNAPTDELRSRLMPVNDKYGLRRLRSVLQNLSRRGRERISLEYILLGGVNDGTEEAEAVAGFARGLKVKVNLIQFNEAKDLPFVPSDEERSRSFQAVLLRHGVPTSIRKSRGQDILAACGQLAREGATLPPARLKPPRHLADLTEAERKDLLVEHGLPGFRAKQLSTHYFSRLVDDPAQMTDLPATQREELVAALLPTLMTPLRTLEADRGTTRKTLWRLFDGALVESVLMRYPGRSTVCVSSQAGCGMACPFCATGQGGLQRNMSTAEIVEQVVAAARTLARGEVPGGPGRVNNVVFMGMGEPLANYKAVLGAVRRLTDPSPAGLGMSARGVTVSTVGLVPRIRQLTEEGVPVTLALSLHAPDDELRNELVPINTRFSVAETVEAAWDYARVTKRRVSIEYAMMRGINDQAWRADLLGDLLNGYGDWGWVHVNLIPLNPTPGSKWTASDPADEREFVRRLEAKGVPTTVRDTRGQEIDGACGQLAQA"
  },
  {
    "reference": "MEHVYAVILAAGAGTRMKSEKAKVAHELCGKPMINWVIDAVKGAGVEEIIVVTGYQEEQVRGCISENVIFTSQKEQLGTGHALMQAMPLLNGRKGSCLVLCGDIPLITAQTLRNLAENHINSGRAMTVLTAVVEDPHGYGRIIRDSMGNACRIVEQKDADEKQRNIKEINSGIYCYDIQWLEESLKKLDNNNMQKEYYLTDTVSIICENGGSVGAITLDNHEEMLGINDRVQLAAAERILNRRIIERHMKNGVTFLIPDSCIVEERVSIGSDTVIYPGTILKGSTSIGSNCVIGPNCMISDTTIDSNTKIIFSVINDSRIGANSTVGPFAYLRPGSIIGEKVKIGDFVEIKKSVIGDETKISHLTYVGDAEIGKNVNLGCGVVFVNYDGKVKNKTIVGDNSFIGCNVNLISPVEVKPNSYIAAGSTITEEVPEYSLAIARSRQTIIRDWVIRRGMIRGEKSDT",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).2. The presence of Mg(2+) is necessary for the protein to function in the desired environment.3. The Proton acceptor of the protein must be engineered to enable effective catalysis of acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate. The designed protein sequence is ",
    "response#1": "VIILAAGKGTRMRSNLPKVLQPLAGRPLLGHVIETAKKLNAENIITIFGHAGNLVQQAFSHENIQWVEQKEQLGTGHAVKVTLPVLPTEGVSLILSGDVPCINPTTLEKLLSVSRETGIGLVTLTLDVEDPKGYGRIVRDANGKVIGIVEEKDATPDQLQILECNTGIMAMNAKYLKEWLSQLNNQNAQGEYYLTDIIAMAHQDGVEIVTTQPDYEFETEGVNNRLQLAELERIYQANHARNLMEQGVHLIDPQRFDLRGELLVGQDVCIDINVIIEGHCEFGNNVQIGAGCIIKNTKIAAGTVVKPYSIFEDANVGENTAIGPFARLRPGTHLANEVHIGNFVEVKNSNIGLGSKANHFTYLGDAEVGAGSNIGAGTITCNYDGANKFKTIIGDHAFIGSNSSLVAPVVIANGATVGAGSTITRDVAENSLAVERSKQRTIKGWQRPTKKK",
    "response#2": "LVLAAGAGTRMKSDTPKVLHTLAGRSMLSHSLHAVAKVAPQHLVVVVGRDRERVAPAALEIGQELGRPVEIAVQEQQQGTGHAAECGLSALPDDFTGVVVVTAGDVPLLDADTLADLIATHSAEAAVATVLTTTLADPTGYGRVLRTQDREVIGIVEQADASPSQQAISEVNAGVYAFDIAALRSALSRLRSDNAQHELYLTDVISIVRSDGAVTAIVEERDASEAQKTITEINDRAHLAEAAAAMRQRIARELQADGVHLIDPQRFDLRGELLVGQDVCIDINVIIEGHCEFGNNVQIGAGCIIKNTKIAAGTVVKPYSIFEDANVGENTAIGPFARLRPGTHLANEVHIGNFVEVKNSNIGLGSKANHFTYLGDAEVGAGSNIGAGTITCNYDGANKFKTIIGDHAFIGSNSSLVAPVVIANGATVGAGSTITRDVAENSLAVERSKQVIVDGWTAPRKGK",
    "response#3": "IILAAGQGTRMRSDLPKVLHPVAGKPMLQRVLETCKKLTNDRLAVVYGHGGEQVKQLTQEDGIVWVLQAEQRGTGHAVAQAIELAPDEAIVLIAYADTPLIRSETLQQLIAHLGDAALAVLTSKPVSPTGYGRIIRNDAGEVTQIVEDKDATAEQKAIQEINTGTYCFDNVYLLSALTHLSSDNAQGEYYLTDIFAMYKASGHKCQAIIADDPEEALGCNDRVQLAAVGAQMRQRINKHWMDEGVTMINPQSISIDDTVSLSPDTVIEPQTHLRGKTYIGSKSHIGPGTFIEDSKIGEHVNISYSVVSHSEISSYCQIGPYAHIRKEVKIHESCRIGNFVEIKKSQVEKNSNIAHLSYIGDASLGEQVNIGAGTITANYDGVNKHPTIIGSQTKTGANSVFVAPVTLGNNVTVAAGSVVTNDVPDNALVIARQQQREISGWVARKRPGTAAADAAKKALEKKDDN"
  },
  {
    "reference": "MAAPFALVSRVSPAARLPIRAAWRRARPTVGLPSSGRARQLAVASAAQENRDNTAVDVHVNQDGGNQQGNAVQRRPRRSSALDGISPFGLVDPMSPMRTMRQMLDTMDRIFDDVALGFPATPRRSLATGEVRMPWDVMEDDKEVRMRFDMPGLSREEVKVMVEDDALVIRGEHKKEEGEGAEGSGDGWWKERSVSSYDMRLALPDECDKSKVRAELKNGVLLVTVPKTEVERKVIDVQVQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.2. The designed protein should be expressed specifically in the chloroplast The designed protein sequence is ",
    "response#1": "LKRSLVDSLYGTDRGLSASSETRAEIGDLITQLESKNPTPAPTDALFLLNGKWILAYTSFVGLFPLLSRGIVPLVKVDEISQTIDSDNFTVENSVLFAGPLATTSISTNAKFEIRSPKRVQIKFEEGVIGTPQLTDSIEIPEYVEFLGQKIDLTPIRGLLTSVQDTATSVARTISSQPPLKFSLPGDSAQSWLLTTYLDKDIRISRGDGGSVFVR",
    "response#2": "LKRSLVDSLYGTDRGLSASSETRAEIGDLITQLESKNPTPAPTDALFLLNGKWILAYTSFVGLFPLLSRGIVPLVKVDEISQTIDSDNFTVENSVLFAGPLATTSISTNAKFEIRSPKRVQIKFEEGVIGTPQLTDSIEIPEYVEFLGQKIDLTPIRGLLTSVQDTATSVARTISSQPPLKFSLPGDSAQSWLLTTYLDKDIRISRGDGGSVFVP",
    "response#3": "LKRSLVDSLYGTDRGLSASSETRAEIGDLITQLESKNPTPAPTDALFLLNGKWILAYTSFVGLFPLLSRGIVPLVKVDEISQTIDSDNFTVENSVLFAGPLATTSISTNAKFEIRSPKRVQIKFEEGVIGTPQLTDSIEIPEYVEFLGQKIDLTPIRGLLTSVQDTATSVARTISSQPPLKFSLPGDSAQSWLLTTYLDKDIRISRGDGGSVFVR"
  },
  {
    "reference": "MWELVALLLLTLAYFFWSKSKTCGAKSPKSLPFLPLVGSLPFIPRHGHPHVNFFKLQEKYGPIYSLRLGSTTTVIIGQYQLAKEVLVKKGKEFSGRPHMVTLGLLSDQGKGIAFADSGGSWQLHRKLALSSFALFRDGNQKLEKIICQKASSLCDFLLTHNEESIDLSEPIFNSITNIICIICFGISYENRDPILATIKSFTEGILNSLGNDHLVDIFPWLTIFPNKTVDMIKKNVKIRDEVLSGILEKCKEKFNSDSISSLMDLLIQAKTNADNNNTSEGQGSNAFSDMHILATIADIFGAGIETTASVLSWIIAFLLHNPEVKKKIQKEIDQNIGFSRTPTFNDRNHLLMLEATIREVLRIRPVAPMLIPHRANSDMSIGEFSIPKFTPVIINLWALHHSEKEWDQPDRFMPERFLDPTGSHLITPSLSYLPFGAGARSCIGEVLARQELFLFMAHLLQRFDLDVPDDEQPPCLKGNANVVFLIDPFKVKITVRQAWKDAQAEVNTWRP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Has 16- alpha hydroxylase activity; Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione; A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis; A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage; Catalyzes 16-alpha hydroxylation of 17- alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA.2. The protein should be optimized for activity in Steroid biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The protein should have a high affinity for heme.4. The ideal protein would be one that plays a critical role in steroid metabolic process, glucocorticoid biosynthetic process, hormone biosynthetic process through steroid 17-alpha-monooxygenase activity, heme binding, iron ion binding, 17-alpha-hydroxyprogesterone aldolase activity within endoplasmic reticulum membrane. The designed protein sequence is ",
    "response#1": "LVTGGCGFLGEHIVRMLLEREPRLRELRVFDLHLSSWLEELKAGPVQVTAIQGDVTQAHEVAAAMSGSHVVIHTAGLVDVFGKASPKTIHKVNVQGTQNVIDACVQTGTQYLVYTSSMEVVGPNIKGHPFYRGNEDTPYEAVHSHPYPCSKALAEQLVLEANGRKVNGGLPLVTCALRPTGIYGEGHQVMRDFYYQGLRFGGRLFRAVPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCGLREPLDLNKCCPYPGPLVTGGCGFLGEHIVRMLLEREPRLRELRVFDLHLSSWLEELKAGPVQVTAIQGDVTQAHEVAAAMSGSHVVIHTAGLVDVFGKASPKTIHKVNVQGTQNVIDACVQTGTQYLVYTSSMEVVGPNIKGHPFYRGNEDTPYEAVHSHPYPCSKALAEQLVLEANGRKVNGGLPLVTCALRPTGIYGEGHQVMRDFYYQGLRFGGRLFRAVPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCGLREPLDLNKCCPYPGPVRMYLL",
    "response#2": "MRDLVTGGGGFLGQRIVHLLLEEKDLQEIRVLDKVFKPEVREEFSKLQSKIKLTMLEGDILDEQCLKGACQGASVVIHTASIIDVVNAVGRETVMKVNVKGTQLLLEACVQASVPVFIHTSSIEVAGPNSYREVIQNACEEDRLETAWSAPYPLSKKLAEKAVLEANGWALQNGGTLHTCALRPMYIYGEGSPFIFAHMNKALENNGVLTHNSKFSRVNPVYVGNVAWAHILALRALRDPRKALSVQGQFYYVADDTPPQSYDDLNYTLGKEWGFCLDSRRSLLVTGGGGFLGQRIVHLLLEEKDLQEIRVLDKVFKPEVREEFSKLQSKIKLTMLEGDILDEQCLKGACQGASVVIHTASIIDVVNAVGRETVMKVNVKGTQLLLEACVQASVPVFIHTSSIEVAGPNSYREVIQNACEEDRLETAWSAPYPLSKKLAEKAVLEANGWALQNGGTLHTCALRPMYIYGEGSPFIFAHMNKALENNGVLTHNSKFSRVNPVYVGNVAWAHILALRALRDPRKALSVQGQFYYVADDTPPQSYDDLNYTLGKEWGFCLDSRRSLLPELSSCPHLEIVLVTGGCGFLGEHIVRMLLEREPRLRELRVFDLHLSSWLEELKAGPVQVTAIQGDVTQAHEVAAAMSGSHVVIHTAGLVDVFGKASPKTIHKVNVQGTQNVIDACVQTGTQYLVYTSSMEVVGPNIKGHPFYRGNEDTPYEAVHSHPYPCSKALAEQLVLEANGRKVNGGLPLVTCALRPTGIYGEGHQVMRDFYYQGLRFGGRLFRAVPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCGLR",
    "response#3": "LVTGGCGFLGEHIVRMLLEREPRLRELRVFDLHLSSWLEELKAGPVQVTAIQGDVTQAHEVAAAMSGSHVVIHTAGLVDVFGKASPKTIHKVNVQGTQNVIDACVQTGTQYLVYTSSMEVVGPNIKGHPFYRGNEDTPYEAVHSHPYPCSKALAEQLVLEANGRKVNGGLPLVTCALRPTGIYGEGHQVMRDFYYQGLRFGGRLFRAVPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCGLRLVTGAGGFLGQRIVQMLVQEKELQEVRVLYRTFSPKHKEELSKLQTKAKVTVLRGDIVDAQFLRRACQGMSVIIHTAAALDIAGFLPRQTILDVNVKGTQLLLDACVEASVPAFIYSSSTGVAGPNSYKETILNDREEEHRESTWSNPYPYSKRMAEKAVLAANGSILKNGGTFHTCALRLPFIYGEESQIISTMVNRALKNNSIIKRHATFSIANPVYVGNAAWAHILAARGLRDPEKSQSIQGQFYYISDDTPHQSYDDLNYTLSKEWGFCLDSSWSLPPEEAQPHDL"
  },
  {
    "reference": "MDPKGTTVLDFIEEQAECSQSDSEQGSEENCSDISDLIDNAEYEQGNSAELFAQQQAFDFHKDICTTKRNLKRSLRNAFQCITSQSNTPASRSAPKRRLLDDSGYNEDISTEVVQVDENGGSEGYGSLTSQHLSGVCLNQGDNGGVDKENVDCTALLRAGSRRAAYLGVFKEKFTISFTALTRIFKNDKTCCRNWVGTVYRAREELLEASKTILQKCCDFILLLTHTCKYGFLALFLLEFKTAKSRETVQRLFEHILQVEKEDMLLEPPKLKSLPAATFWWKIQHSNNSFKWGTLPDWIARQTMISHQIADDEPFSLSVMVQWAYDHNYTEESTIAYHYAKLASEDSNAAAFLKCNNQVKHVKECAQMTRYYKTAEMTEMSMGQWIKKCIGEIEGVGDWKQICKFLKFQNVNFLSFMSALKDLLHRVPKRNCMVICGPPNTGKSMFVMSFMKALQGKVLSFVNSKSHFWLQPLRGAKVAVLDDATRATWTYFDTYLRNGLDGTPVSLDMKHRAPLQICFPPLVITTNVNVMQDPAYFYLHSRIVCFEFPNTFPLDEAGNPLLLIDELSWKSFFERLWTQLDLTDAEEDEDGEPRSPFRCCPRSVATSL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. The designed protein sequence is ",
    "response#1": "STFDFSVMVQWAFDNNHVDEADIAYQYARLAPEDSNAVAWLAHNSQAKFVRDCAAMVRFYKNLQMREMSMSEWIYTRINEVEGEGHWSSIVKFLGYQGVNFIMFLAALKNFLHAVPKQNCILIHGPPNSGKSSFAMSLIKVLKGRVLSFVNSRSQFWLQPLGECKIALIDDVTDPCWLYMDTYLRNGLDGHFVSLDCKYKAPVQTKFLPLLLTSNINVHEETNYRYLHSRIKGFEFPNPFPMKSDNTPQFELTDQSWKSFFERLWTQLELSDQEEEEEGEHGETQRAFQC",
    "response#2": "KFKDAFGVGFNELTRQFKSYKTCCNHRVLAVYAVHDDLFESSKQLLQQHCDYIWVRGIAAMTLYLLCFKAGKNRGTVHKLLTSMLNVQEQQILSEPPKLRNTAAALFWYKGGMGSGAFTYGKYPDWIAQQTVLGHV",
    "response#3": "KFKEAFGVGFNDLTRQFKSYKTCCNHWVLSVYAVHDDLLESSKKLLQQHCDYVWIRGIAAMSLFLLCFKVGKNRGTVHKLMTSMLNVHEKQILSEPPKLRNVAAALFWYKGAMGSGTFTYGPYPDWMAHQTIVGHC"
  },
  {
    "reference": "MRVRGTQRNWPQWWIWGILGFWMIIICRGVGNSWVTVYYGVPVWREAKATLFCASDAKAYKTEVHNIWATHACVPTDPSPQEIWLQNVTENFNMWKNDMVDQMHEDVISIWDQSLKPCVKVTPLCVTLNCVDVPINNTRYNESGTERNMMEEFMKGEGMNMTGEIKNCTFNTTTEIRDRKQKAYALFYRPDVVPLNGDNSSYILINCNTSTITQACPKVTFDPIPIHYCAPAGYAILQCNNKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEKEIIIRSENLTDNAKTIIVHLNESIKITCVRPNNNTRKSIRIGPGQAFYATNEIIGDIRQAYCNISGKEWNKTLEKVRGKLKEHFNTTILFGNHSGGDLEVTTHSFNCRGEFFYCNTTQLFNENITRMFNESTTNENITIPCRIKQIINMWQEVGRAMYAPPIAGKITCISNITGLLLERDGGNNRTNETFRPGGGNMKDNWRSELYKYKVVEIKPLGIAPTEAKRRVVNRGKRAVVGIGAVFLGFLGAAGSTMGAAALTLTVQAKQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGLWGCSGKLICNTDVPWNSSWSNKSQDLIWDNMTWMEWDREISKYTDSIYKLLEDSQRQQEQNEQDLLALDSWKNLWNWFDISNWLWYIKIFIMIVGGLIGLRIIFGVLSIVKRVRQGYSPLSFQTLTPNPRGLDRLGRIEEGDGEQDKDSSIRLVNGFLALAWDDLRSLCLF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design must feature the YXXL motif; contains endocytosis signal motif.2. The presence of Retroviral envelope protein GP41-like domains is critical for the interaction of the designed protein with its intended targets.3. The designed protein should have at least Helical transmembrane domains to ensure proper localization.4. The protein must exhibit the following characteristics: The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Transmembrane protein gp41: Acts as a class I viral fusion protein.5. The designed protein should contain Cytoplasmic topological domains.6. The protein must contain a signal peptide for proper functionality. The designed protein sequence is ",
    "response#1": "MRVMGIQRNCQHLLTWGTMILGMIIICSATENWVTVYYGVPVWRDAETTLFCASDAKAYDTEAHNVWATHACVPTDPNPQEIHLKNVTENFNMWKNAMVEQMNEDIISLWDQSLKPCVKLTPLCVTLHCHNFSNTSSSNSLHSSTVSPQMRGEIQNCSFNTTTLLKDKKKKEYALFYRQDIVPLDTNGTNIDTNGTNSSQYMLINCNTSTIAQACPKITFEPIPIHYCAPAGFAILKCRDKEFNGTGTCKNVSTVQCTHGIKPVVSTQLLLNGSTAEEGIVIRSKNISANAKTIIVQLDEPVRINCTRPGNNTRKSVHLGPGQAFYATGDIIGDIRQAYCNVSGTEWNKTLHQVATKLKGYFKSNTTIIFTKPSGGDLEITTHSFNCGGEFFYCNTSNLFNGTLYNNTWMFNGTLYNNTNESSTSGTITIKCRIKQIINMWQTVGQAMYAPPIPGEIRCESNITGLLLTRDGEAGKSTNETFRPIGGNMRDNWRSELYKYKVVKIEPLGVAPTPAKRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVESYLRDQQLLGIWGCSGKLICTTTVPWNASWSNKSYSEIWENMTWLQWDKEISNYTNLIYGLIEESQNQQEKNEQDLLALDKWANLWSWFNISNWLWYIRIFIIIVGGLIGLRIVFAVLSIINRVRQGYSPLSFQTRLPAQRGPDR",
    "response#2": "MRVMGIRKNYQHWWRGGILLLGILMICSATEKLWVTVYYGVPVWKEASTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQELRLENVTENFNMWKNNMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDYLNATATWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVELNVTENFNAWKNNMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTELKNNNTNNTTMNNTSSIKEGEMKNCSFNVTTAIRDKVQKQYALFYRLDVVQIKDNDTSYRLINCNSSVITQACPKVSFEPIPIHYCAPAGFAILKCKDKNFNGTGLCKNVSTVQCTHGIRPVVSTQLLLNGSLAEGDVVIRSKNFSNSAQTIIVQLKETVEINCTRPNNNTRRSIHIGPGRAFYATGDIIGDIRQAHCNISRAKWNETLKKIADKLREQFKRETIVFNQSSGGDPEIVMHSFNCRGEFFYCNTTQLFNSTWNSTTGVNGTGANNTEVNIITLPCKIRQIVNMWQEVGKAMYAPPISGQISCSSNITGLLLLRDGGNSNDTDNTTEIFRPGGGDMRDNWRSELYKYKVVQIEPLGVAPTKARRRVVQREKRAVGIGAVFLGFLGAAGSTMGAASVTLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICPTAVPWNTSWSNKSLDQIWQNMTWMEWEREINNYTDLIYSLIEESQNQQEKNEQELLALDKWASLWNWFDISKWLWYIKIFIMIIGGLIGLRIVFAVLSIVNRVRQGYSPLSF",
    "response#3": "MRVRGIQRNWQQWWIWGILGFWMLMMCNVRGNLWVTVYYGVPVWRDAETALFCASDAKAQVEEVHNVWATHACVPTDPNPQEIHLENVTENFNMWKNSMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCSN"
  },
  {
    "reference": "MASSSFSVTSPAAAASVYAVTQTSSHFPIQNRSRRVSFRLSAKPKLRFLSKPSRSSYPVVKAQSNKVSTGASSNAAKVDGPSSAEGKEKNSLKESSASSPELATEESISEFLTQVTTLVKLVDSRDIVELQLKQLDCELVIRKKEALPQPQAPASYVMMQQPNQPSYAQQMAPPAAPAAAAPAPSTPASLPPPSPPTPAKSSLPTVKSPMAGTFYRSPAPGEPPFIKVGDKVQKGQVLCIVEAMKLMNEIESDHTGTVVDIVAEDGKPVSLDTPLFVVQP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA..2. The presence of Biotinyl-binding domains is critical for the interaction of the designed protein with its intended targets.3. Target a Polar residues, Pro residues compositional bias in the protein's composition for improved properties.4. The protein design should take into account the physiological conditions of the fatty acid biosynthesis, Lipid metabolism pathway.5. A protein that localizes to chloroplast envelope, acetyl-CoA carboxylase complex, plastid, chloroplast stroma, chloroplast and can perform acetyl-CoA carboxylase activity with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "MTSDERIDDLLDAGTFQEWDRVIEQTDPLGFPGYLDKLEAQRGKTGLEEGVRTGQGSIAGLKCAIGIMESTFFMGSMGSVVGEKVTRLFERATAEHLPVIIFTASGGARMQEGLVSLMQMAKTSCAIERHAAAHLPYISVITDPTTGGVTASFAMDGDIILAEPKALIGFAGQRVIREPESCNSIPEEMYRSACVSTTEEAVASCQVVGYPAMIKASWGGGGKGIRKVHNDDEVRALFKQVQGEVPGSPIFIMKVASQSRHLEVQLLCDKHGNVAALHSRDCSVQRRHQKIIEEGPITVAPSETVKELEQAARRLAKCVHYVGAATVEYLYSMETGEYYFLELNPRLQVEHPVTEWIAEINLPAAQVVVGMGAFDTEIQALRDRAQELRKEAYANLDAWQKTMVARHPQRPHLRDYVAGLIDEFVELRGDRKFADDQAIVGGLGRFRGQPVVVMGHEKGHDTTTRLKHNFGMARPEGYRKAVRLMDMAERFNLPVITFVDTAGAYPGLGAEERGQAEAIARSTERCLTLGVPMVATIVGEGGSGGAIALAGANRVLILEHSIYSVISPEGAASILWRDGARAKDAATQMRITAQDLIKLGIVDRIVEEPAGGAHSDTEAAIQAVGDAVEDELKSADLAAASGWDTVKLARHIDRPTGLDHVAHLLDGFVELHGDRLGADCPALVGGFGVLDGLPVLALCHQKGHNTAELVARNFGMPSPAGYRKSARLLRLAAKLGTPVVALIDTPGAFPGIEAEENGQAVAIAENLRLMGGLGVPVVSVITGEGGSGGALALAVADTVLITQNGTYSVISPEGCASILWRDAAAAPLAAASLGIDPVSLLRAGVVDAVVPEPDGGSHLDPAAASELLRQAVARSLA",
    "response#2": "MTSDERIDDLLDAGTFQEWDRVIEQTDPLGFPGYLDKLEAQRGKTGLEEGVRTGQGSIAGLKCAIGIMESTFFMGSMGSVVGEKVTRLFERATAEHLPVIIFTASGGARMQEGLVSLMQMAKTSCAIERHAAAHLPYISVITDPTTGGVTASFAMDGDIILAEPKALIGFAGQRVIRPESCNSIPEEMYRSACVSTTEEAVASCQVVGYPAMIKASWGGGGKGIRKVHNDDEVRALFKQVQGEVPGSPIFIMKVASQSRHLEVQLLCDKHGNVAALHSRDCSVQRRHQKIIEEGPITVAPSETVKELEQAARRLAKCVHYVGAATVEYLYSMETGEYYFLELNPRLQVEHPVTEWIAEINLPAAQVVVGMGEPRTEVLSELDELRKKFKEKARKIYRDLSPWERVQVARHPKRPHTSDYIKYLIKDFEEVHGDTCYGDDKAVIAGFGYFRGKPVAVVGHEKGKDTKEKLERNFGMPHPEGYRKAIKVFKLAERYNIPVITFIDTPGAFPGIGAEERGQSRAIAESMLTMAFLKVPSVAVVIGEGGSGGALAFGVANRVCILENAYYSVISPEGCAAILWKDQSKVKEAAKALRLTAKDLKELGVVDCVIPEPYGAAHWSPRGTAMMVGMTLKKCLDEAGLTPRLIDELIAAASPDEQENRAWHSVQLARNVHRPTALSYIRSFVDGFVELHGDRGFADDGAIVGGIGWIGGRAVTVIGQEKGENLKERIRRNFGCPQPEGYRKALRLMRQAEKFRRPIVCLVDTQGAYCGTEAEERGQGNAIADNLVAMAGLKVPVVTVLLGEGGSGGALALAVANRVAMQEHAVYSVLSPEGFASIL",
    "response#3": "MQKSGKSEACRNATGKLGGSDAVISGMDFGFIGGSMGSVVGEKIARAADKSVELNAPLIVISQSGGARMMEGAFSLMQMAKTSARLTRLGERGIPFISLMTDPTMGGISASFAMLGDLNISEPKALIGFAGPRVIRDPTKIPESCNSIPEEMYRSACVSTTEEAVASCQVVGYPAMIKASWGGGGKGIRKVHNDDEVRALFKQVQGEVPGSPIFIMKVASQSRHLEVQLLCDKHGNVAALHSRDCSVQRRHQKIIEEGPITVAPSETVKELEQAARRLAKCVHYVGAATVEYLYSMETGEYYFLELNPRLQVEHPVTEWIAEINLPAAQVVVGMGEVLSELDELRKKFKEKARKIYRDLSPWERVQVARHPKRPHTSDYIKYLIKDFEEVHGDTCYGDDKAVIAGFGYFRGKPVAVVGHEKGKDTKEKLERNFGMPHPEGYRKAIKVFKLAERYNIPVITFIDTPGAFPGIGAEERGQSRAIAESMLTMAFLKVPSVAVVIGEGGSGGALAFGVANRVCILENAYYSVISPEGCAAILWKDQSKVKEAAKALRLTAKDLKELGVVDCVIPEPYGAAHWSPRGTAMMVGMTLKKCLDLDGSVHKPEVLSELDELRKKFKEKARKIYRDLSPWERVQVARHPKRPHTSDYIKYLIKDFEEVHGDTCYGDDKAVIAGFGYFRGKPVAVVGHEKGKDTKEKLERNFGMPHPEGYRKAIKVFKLAERYNIPVITFIDTPGAFPGIGAEERGQSRAIAESMLTMAFLKVPSVAVVIGEGGSGGALAFGVANRVCILENAYYSVISPEGCAAILWKDQSKVKEAAKALRLTAKDLKELGVVDCVIPEPYGAAHWSPRGTAMMVGMTLKKCLD"
  },
  {
    "reference": "MKKKIFLVAGGSGGHFFPALALYKSCKEKKFDFIFLVDDRVEVLAKNNSCKFNNVLTPRFTGSIKQLIKNIFGVFSSIIKTTYLYIKTKPDLVVGFGGYTSIFPIVLAKIFQIKTLIHEQNAILGKANRFLSKFNTKVALTFKKTQFAPKNSKFTGIPVRKKIHIIKKNKKKVFLIIGGSQGARVFANIVPEILKFLNKKYKKEILVIQQVRNEDYLRLKKKYQELGINFVLRKFFKNIYQKISIADVVMSRCGSSTLAELELFMKFAILIPLPTSMEDHQYLNAREFKKKNECLIVNEKKIDYCYIARVLQSKFFEKKKISENESSISQEFFIRFSQRRSIIMIGKKKSIHFIGIGGIGMSAIAEILFKLGFKVSGSDINQNLIIRKLSKKGIKIFSSHSSKNVQKADMVVFSSAIKKNNKELKFAQEKKIPILSRASMLAEVMRLKSSITVAGSHGKTTTTSLIATILEKAGFDPTIINGGIINSFQTNAKLGKGKWLVAEADESDGSFVCLPSTIGLINNIDLEHIDFYKNLNQIKAAFVEYAKNIPFYGFIALCIDSKNVKDIKEKLFEKKIFSFGLNDKANFYASDVKIVEIKKRFFSKFDVIINLKKKEKIKNIIVPIIGNHNIQNILGSICVAKGLGISNLSIKQGLKNYEGVRRRFSIISKNDNNMIIDDYAHHPVEIKKTLESLKLITRKRIFSIFEPHRFSRITKFLDEFAMSFKQSDFIYILPIYSAGEQNRQKITPNTIENTLKKKFKNKVIKSVN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein with UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase activity is needed to enhance carbohydrate metabolic process, regulation of cell shape in a specific context.2. The designed protein have Mur ligase C-terminal domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "VTGTNGKSTVTQLLAQATTKLGQPCAVIGTLGVGFFGDLQESTHTTPDPIAVQAGLAKLKAQGARAVAMEVSSHALDQGRVTAVEFDVAVFTNLTRDHLDYHGDMQSYAAAKAQLFHWRSLQAAVINQDDALGRQLLAEIKTAKCYSYSCEDPSATVFAKNVRFSHLGIQAEIVTPHGEGVLRSPLLGAFNLSNLLAVIAALREAGVAFDDIAAALASFAGAPGRLELAGSYNGAPIFIDYSHKPDALAKALEALRPYASGHLVVVFGAGGDRDPGKRPLMGEVAQQKADRVIVTDDNPRSEEPAEIRAAILAAAPGATEIGDRGQAIRAGIAGLRPGDVLLVAGKGHEPYQEVAGVKHPFDDAEQAEKALAARKGV",
    "response#2": "GRMQRVDAGQDFLAVVDYAHKPAAVGALLDALRAQVDGRVLTVLGCGGDRDRGKRPLMGAIAAAGSDVLVVTDDNPRTEDPAEIRRQMRAGAEGEPGRGEILEIGDRRAAIAAAVAAARPGDVVVVAGKTPIQGRS",
    "response#3": "MKLIVFTGGGTVGHVTLNLLLIPRFLEEGWEVHYIGDGNGIEHEQVVKSGLDVHFHSIATGKLRRYFSFQNMLDVFKVGFGVLQSLTIIAKIRPQALFSKGGFVSVPPVIAANLLRVPVFIHESDLTMGLANKIAYKFATTMLSAFPHFASKTRVTGNPVRPAILDLPLPDAQRYAGRAGPLNTLLFVGGSGGAKVFNDLISQNSAALTERFNIINLTGDSSLNKLDKNLYRVDYVTELYQPLMDLADVVITRGGSNTLFELIAMQQLHLIVPLGRQASRGDQIENALYAEKKGYSKQIDESQLTFASLLVEVDELLKHKEFYVQNMANSNWEEMARACIKVTGTNGKTSCTQWLSQVLPQCAWMGTLGLGWGQQSRGGYLTTPDAITVQKNLRWVQQQGMSTVVMEVSSHALDQGRVAGVSFVGAVLTQITSDHLDYHKTQEAYVRAKERLFCWDSLGFMVLNRDDAMGQRWMGRHRERSIPIWTYGLGPSNRKGDNSVAVSEVSLQADGVRFKGLFRGRASWFEVPVLGRFNMFNVLAVMTCAELLLEKPMTLERITEALDKFTGVTGRLQCHKLKNGAMAFVDYAHKPDAMEKVLKTLRPQTDHLIVVFGCGGNRDKTKRSVMGLCAANYADQIIITDDNPRDEDRQVIVDEIMAGIPEIKKSCTQCILDRASAIKTAAQISNPNSIIALLGKGNEQGQIIGSDVVPFDDVAVARRAVADRHE"
  },
  {
    "reference": "MYKLVLVRHGQSTWNLENRFTGWTDVDLTPLGETEAREGARLIAAAGLTFDECHTSVLKRAIRTLNIIQEELDLLWLPVHRHWRLNERHYGALQGLNKKEMSEKYGEEQVFIWRRSFDTPPPALTADDPRHPAHDRRYASLTARQMPLCESLKDTVARVLPYWHETLAPAIQAGRRLLVAAHGNSLRALVMHLDGMSPDEVTGLNIPTGLPLVYELDSNLTPLRRAYLGDAEAAEAKARAVAEQAKAGTTERP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The substrate binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must exhibit the following characteristics: Catalyzes the interconversion of 2-phosphoglycerate and 3- phosphoglycerate..3. The designed protein should be optimized for 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity activity in order to improve glycolytic process, gluconeogenesis.4. A protein that is capable of catalyzing reactions within the pyruvate from D- glyceraldehyde 3-phosphate: step 3/5, Carbohydrate degradation pathway with high efficiency and specificity.5. The Proton donor/acceptor, Tele-phosphohistidine intermediate active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "INKAIEEGTLPENDALKNAIQHVKDNNSALHLMGLLSDGGVHSHINHLFGLLEMARVKNIEKVYVHPILDGRDTPPRSAEKYLNQLEEKLAELGIGEIATVSGRYYTMDRDNRWDRTKKSYDALVLGEGLEASDAISALKQSYAADKSDEFVIPTVIKKNGQPVGTIKDNDAVIFYNFRADRARQITKALALEEFDEFERPAEHPENLYYVCMAEYDAH",
    "response#2": "INKAIEEGTLPENDALKNAIQHVKDNNSALHLMGLLSDGGVHSHINHLFGLLEMARVKNIEKVYVHPILDGRDTPPRSAEKYLNQLEEKLAELGIGEIATVSGRYYTMDRDNRWDRTKKSYDALVLGEGLEASDAISALKQSYAADKSDEFVIPTVIKKNGQPVGTIKDNDAVIFYNFRADRARQITKALALEEFDEFERPAEHPENLYYVCMAEYDGE",
    "response#3": "INKAIEEGTLPENDALKNAIQHVKDNNSALHLMGLLSDGGVHSHINHLFGLLEMARVKNIEKVYVHPILDGRDTPPRSAEKYLNQLEEKLAELGIGEIATVSGRYYTMDRDNRWDRTKKSYDALVLGEGLEASDAISALKQSYAADKSDEFVIPTVIKKNGQPVGTIKDNDAVIFYNFRADRARQITKALALEEFDEFERPAEHPENLYYVCMAEYDVKTAGATINKAIEEGTLPENDALKNAIQHVKDNNSALHLMGLLSDGGVHSHINHLFGLLEMARVKNIEKVYVHPILDGRDTPPRSAEKYLNQLEEKLAELGIGEIATVSGRYYTMDRDNRWDRTKKSYDALVLGEGLEASDAISALKQSYAADKSDEFVIPTVIKKNGQPVGTIKDNDAVIFYNFRADRARQITKALALEEFDEFERPAEHPENLYYVCMAEYDVKTAGAT"
  },
  {
    "reference": "MSNVTENKHDLHENGVPDSAQPMELDVSKKEDTEPEVRDEAKEFLLSQLPQVEVPEWAQWFDFSKVHEIEKKQNPEFFDGKNTSKTPEVYKEYRDFMISTFRLNSKVYLTFTACRRNLAGDVCAVLRVHRFLEQWGLINYNVNPDTRPSKIGPPSTSHFQILADTPRGLVPLLPPPSSSIPRSKAVTIEDPSIVRTNIYDPSLDDVLKGKGSTPNQKPSLSNLHENNIDQSDSPQHCYCCGNKFNESYYQSQTAQKYNVCISCYQQNRFPSPTTIADYKEVAIQNKIEDDDTWTAQELVLLSEGVEMYSDDWAKVASHVNTKSVEECILKFLNLPSSDKALFKMDKVHTNPVVDSLQGKNPILSVVSFLAKMVPPSSFTQKSSAKEEESDKVKGESVYPKPESESYDVEMNGKSLEDSDSLSELYLTNEEKKMASIIKDSVNVQIKLIESKLSHFDYLDQHIRLKSQELDAFAQATYREKLYMKRECQNARKKIEQQLQSKDTAANGLPVQSSAETSVIPASSMSPS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that can perform DNA binding in cytosol, nucleus, chromatin, RSC-type complex to support regulation of transcription by RNA polymerase II.2. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.3. For general function, the protein need meet that Part of the SWI/SNF complex, an ATP-dependent chromatin remodeling complex, required for the positive and negative regulation of gene expression of a large number of genes The designed protein sequence is ",
    "response#1": "MVGQLAARRAAGVVLEMIREGKIAGRAVLIAGQPGTGKTAIAMGMAQALGPDTPFTAIAGSEIFSLEMSKTEALTQAFRRSIGVRIKEETEIIEGEVVEIQIDRPATGTGSKVGKLTLKTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSPHGIPIDLLDRLLIVSTSPYSR",
    "response#2": "ALKSAAQKAKLIAAFENRQLRRLVFSLIQAQLEKLQLKMKVLEQLEKMCSLELSELDLRGKNLLLSRLSTKKMLLAFNKKK",
    "response#3": "CGLSAAATKAKLFADQEEREIQRLTATVINHQLKRLELKLKQFAEVETLLLKECEQVERIRQRIASDRVRIVSTRC"
  },
  {
    "reference": "MADDAIRAEVAERLTRAGENGVLRVITCGSVDDGKSTLIGRLLYDSKLLFEDQIKVLERDSRKHGTAGEEIDFALLLDGLEAEREQGITIDVAYRFFNTEKRKFIVADTPGHEQYTRNMATGASTADLAIILVDARKGVLTQTKRHTYIANMMGIRHAVLAVNKMDLVEHSRARFHEIVSEYLTIAEDLGFATITPIPISARYGDNIFSRSDAMKWHDGPTLMEHLERVDTNTEKEAAYAFRFPVQWVNRPNLDFRGFSGTVASGAVAPGDDVVVAHSGKAAKISRVVTADGDLETASAGDAVTLVLDREIDIARGDMLADPQQRPEVSDQFAAEILWMTEEEMLPGRSYLLKAGGQVSPVSVTTLKHKLNVNSFEKEPGKSLKLNEIGVCTLSTSRPIAFDPYKDQRETGSFILIDRFTNQTVGAGMIEFSLRRASNIHTHAMDVDKARRADMKGQKPAVLWFTGLSGAGKSTIANLVERKLAEAGRHTYSLDGDNVRHGLNKDLGFTDVDRVENIRRIGEVAKLFVDAGLIVTCSFISPFRSERQMVRELVDEAEFIEIFVDAPLEVCMQRDPKGLYKKAQAGEIKNFTGFDSPYEAPESAEIHLKTGDLSAEDAAERVIETLIERGFIG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The Phosphoserine intermediate active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The designed protein must have at least one Tr-type G domain.3. A protein with sulfate adenylyltransferase (ATP) activity functions is required to enable hydrogen sulfide biosynthetic process. The designed protein sequence is ",
    "response#1": "KTMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLEALQSDSKKVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFNTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLVQTRRHSYLAHLIGIKNIVLAINKMDLVDYDQSVYDAILKDYTEFATSIGITAFTPIPISGFKGDNITTLSENTPWYTGPSLIEHLETVEVNSALKSLLRFLTCGSVDDGKSTLIGRLLSDTKQIFEDQLAALERDSRKHGTTGEDVDFALLVDGLEAEREQGITIDVAYRFFATAKRKFIVADTPGHEQYTRNMATGASTADLAIVLVDARQGVLRQTRRHSIIASLLGIKHIVVAINKIDLVDFSQEAFDKITADYQAFAESLGFKSIVPIPMSARFGDNVTRRSEKMGWYSGPTLLEHLESVVVEEDATRRLRARIFWLHDQPLTAGSSILVRLGVREVRGRVVAIEKAIDPGELASVETKQIARNHVGEIEIALSQPVAADRYVDNPRTGRLVIEVGGRIAGGGLFEADIVWMHDTAMETGKLYDLKLATRDLAGQVTAIDYQVDVNTLEHKRADHLDLNAIGRCRVELTAPIPLDDYRQSPGTGSFIVIDRLTNVTVGAGMIV",
    "response#2": "GSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFATEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSYLAHLIGIRNIVLAVNKMDLVDYDLAIFDAIVKDYREFADSIGIESFTAIPISGFKGDNIRFITCGSVDDGKSTLLGRLLFDAGMLPDDQVEALRRDSRRQHPDGDVLDFSLLTDGLDAERQQGITIDVAYRYFHSARRNFIVADCPGHEQYTRNMATGASHADVAVVLVDARKGLLPQTRRHTYICALLGIHKIILAVNKMDLVDYAKEVYEPIKEAYINLAKELGIAAVHALPVAALTGDNVGAPSKDMPWYEGPTLLDLLETIHVEKSLLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFATEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSYLAHLIGIRNIVLAVNKMDLVDYDLAIFDAIVKDYREFADSIGIESFTAIPISGFKGDNITTTPSPNTPWYKGPALIAHLESVEIDNHASQELDAMVCWLTDRTELTPGTKFVIKHTTRSTRTLVRSVDYRLDITTLHREEHADSLALNEIGRISLRTQEPLFFDEYRRNRATGGFILIDEQTGGTVAAGTFEASLLWMDENELTAGRDFWLKLGTQLVPVTITRILYKIDVDNGEEVQTDRICKNENAVCELAVGKKIVLEPFAENNMLGAFILIDRLRNTTAAGGA",
    "response#3": "RFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFATEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSYLAHLIGIRNIVLAVNKMDLVDYDLAIFDAIVKDYREFADSIGIESFTAIPISGFKGDNITTTPSPNTPWYKGPALIAHLESVEIVSA"
  },
  {
    "reference": "MAAAAATTSAAAAAAAATQAPAARGDGASSVHWFRKGLRLHDNPALLAAVRGAHCVRCVYILDPWFAASSSVGINRWRFLLQSLEDLDRSLRKLNSRLFVVRGQPADVFPRLFQEWGVTRLTFEYDSEPFGKERDTAIMKMAKEAGVEVVTENSHTLYDLDKIIELNGQKPPLTYKRFQAIISRMELPRKPVGSVTSQQMEGCRAEIQESHDETYGVPSLEELGFPTEGLGPAVWQGGETEALARLDKHLERKAWVANYERPRMNANSLLASPTGLSPYLRFGCLSCRLFYYRLWDLYKKVKRNSTPPLSLFGQLLWREFFYTAATNNPRFDRMEGNPICIQIPWDRNPEALAKWAEGKTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWVSWESGVRVFDELLLDADFSVNAGSWMWLSCSAFFQQFFHCYCPVGFGRRTDPSGDYIRSAGICPFLCGRPQQPSGRAQLDPGRELKQCRPKTTARGPSVSQAQTGSSRGAAWWRAQQTGQGGRVAPLRTAEQGRLRLQCRGSVSKAWVPKPPPWQRSATCRAADHLQRRSERVCVCVRVRRKEGHLLCVHASVYTRVSLNAVWAGGGRVAASPQPPDPGLEVTAVAVTPYLSLGACCLFCLEGWSDLRWDSDTCSPSPSPSMSD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein can be targeted to extracellular region and perform phosphatase binding. The designed protein sequence is ",
    "response#1": "SNFFVPLKRGEPIKKLGEMVGDFPFKKKTLPPGGIFAPFDRKEETRGGEANLKLFGEFASCLIFQVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDVLMHLQN",
    "response#2": "PTYRYHRLPLPEQGSPLEAQLDAFVSVLRETPSLLQLRDAHGPPPALVFSCQMGVGRTNLGMVLGTLILLHRSGTTSQPEAAPTQAKPLPMEQFQVIQSFLRMVPQGRRMVEEVDRAITACAV",
    "response#3": "PTYRYHRLPLPEQGSPLEAQLDAFVSVLRETPSLLQLRDAHGPPPALVFSCQMGVGRTNLGMVLGTLILLHRSGTTSQPEAAPTQAKPLPMEQFQVIQSFLRMVPQGRRMVEEVDRAITACAR"
  },
  {
    "reference": "MSSQTKYIFVTGGVSSSLGKGIIAASLAKLLQAQGYKTTIQKLDPYINVDPGTLNPYEHGECYVTNDGAETDLDLGHYERFLNVPTSQANNITTGRIYQNVISKERQGKYLGKTVQVIPHITDEIKENIKFLESKGEYDIIITEIGGTVGDIESLPYIEALRQLKWEMGEDNLIVIHLTLIPYLSAAKELKTKPTQHSVKTLMESGVQADILVCRTEHELSNEIKEKLARFCNVNVDCVIESIDVNTIYDVPNHMHHEGLDKVALTKLKLNYNKPNLIAWNDFLNKYKNPKDSINIALIGKYVELQDSYKSILESFIHAGAINEVDVNIVPLHSEHITSANVQEKLESFNGILVAPGFGERGVEGKIEAIKYVRLKKIPFFGICYGMQMAVIEYARNVLKLVDANSTEVNPSTKNPVIDLMEEQKSITDKGGTMRLGSWKCNVKKETLCHSVYKSENIDERHRHRYELNNKYLNMLEDNGLVASGKNNDTNLVEVVEIKDHPWFLGVQFHPEYKSTVLSPHPLFVSFVNAAILNKNN",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen2. The protein should be able to bind L-glutamine ligand in a variety of conditions3. The designed protein should be optimized for CTP synthase activity activity in order to improve glutamine metabolic process, 'de novo' CTP biosynthetic process. The designed protein sequence is ",
    "response#1": "LNISLVGKYIELYDAYYSVTEALDHASIAHGVMPVIDWISAEDLEGLSKAEVADRFKDSDAILVPGGFGPRGMEGMIAAAAAARDNNIPYLGLGLGMQMAVVEYARNVTGIRDAHTDECEAPCSDIFYYAGLTDEMIQNLSAIPYSEQPMRIGAYPMRLQEGSRLASIYKSLETSERHHHRRELNPAFQAELSASGMLFSGTSPDGLLVEAFELPGHPCYMGIISHPEFKSRPYRPHPLFDELL",
    "response#2": "ITIHVVGKYVGYEDSYKSLNEALYHGGLPHRLKVNIKWMEAEALEQPGGEALLDDADGILVPGGFGDRGTRGMMKAAQIARERGIPYFGICYGFQWATVEYARNVAGLPDADSTECAPETPTKVIYKLRDLLGVDEMGGTMRLGSYACELAPGSLAHRVYGVGTIHERHRHRYEFNCLYERTLSDKGLRISGRSPDGKFVEIAEVPDHPWYVAVQFHPEFTSKPTRPHPLFASFR",
    "response#3": "VTIAFVGKYLDLKESYKSLTEALIHAGAHINAKINIHWCDSERIEDAGAYEVIQTCDAVLVAGGFGHRGVEGKLKAIEYARTNNIPYLGICLGMQLAVIEYARNVLGIEDANSVEFDPEAKNPLIYLIDEFLDQSGNKQVRTHESPMGGTMRLGEYPFTPKKGSKLQKAYGDKETYYERHRHRYEANPKYKEALEKAGMIISGESHGLIEAVELKDHKWFVGVQFHPEFTSHLETPNPIILEFIKAAN"
  },
  {
    "reference": "MAVKRPVRVLVDMDGVLADFESGLLQGFRRRFPEEPHVPLEQRRGFLANEQYGALRPDLAEKVASVYESPGFFLNLEPIPGALDALREMNDMKDTEVFICTTPLLKYDHCVGEKYRWVEQNLGPEFVERIILTRDKTVVMGDLLIDDKDNIQGLEETPSWEHILFTCCHNQHLALPPTRRRLLSWSDNWRGIIESKRASL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should prioritize the formation of strand-turn-helix-helix-helix-helix-turn-helix-strand-turn-helix-helix-strand-helix-strand elements.2. The designed protein should have a binding site that is specific to the target substrate ligand.3. The designed protein must have a Nucleophile, Proton donor active site that is highly conserved among related enzymes.4. A protein that can perform nucleotidase activity, 5'-nucleotidase activity, phosphatase activity, pyrimidine nucleotide binding in cytosol, cytoplasm, mitochondrion, nucleus to support pyrimidine deoxyribonucleotide catabolic process, IMP catabolic process, dGMP catabolic process, dTMP catabolic process.5. The protein must bind to Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MESTFIMIKPDGVQRGLIGEIISRFEKKGFYLKALKLVNVERSFAEKHYADLASKPFFQGLVDYIISGPVVAMVWEGKSVVTTGRKIIGATNPLASEPGTIRGDFAVDIGRNVIHGSDSIESANKEIALWFPEGS",
    "response#2": "MPELPEVETVRRELEKRIVGQKIISIEATYPRMVLTGFEQLKKELTGKTIQGISRRGKYLIFEIGDDFRLISHLRMEGKYRLATLDAPREKHDHLTMKFADGQLIYADVRKFGTVRPAEVIDEEPELPEVETVRRELEKRIVGQKIISIEATYPRMVLTGFEQLKKELTGKTIQGISRRGKYLIFEIGDDFRLISHLRMEGKYRLATLDAPREKHDHLTMKFADGQLIYADVRKFGTWPELPEVETVRRELEKRIVGQKIISIEATYPRMVLTGFEQLKKELTGKTIQGISRRGKYLIFEIGDDFRLISHLRMEGKYRLATLDAPREKHDHLTMKFADGQLIYADVRKFGTWPELPEVETVRRELEKRIVGQKIISIEATYPRMVLTGFEQLKKELTGKTIQGISRRGKYLIFEIGDDFRLISHLRMEGKYRLATLDAPREKHDHLTMKFADGQLIYADVRKFPELPEVETVRRELEKRIVGQKIISIEATYPRMVLTGFEQLKKELTGKTIQGISRRGKYLIFEIGDDFRLISHLRMEGKYRLATLDAPREKHDHLTMKFADGQLIYADVRKFGTWREGLPPELPEVETVRRELEKRIVGQKIISIEATYPRMVLTGFEQLKKELTGKTIQGISRRGKYLIFEIGDDFRLISHLRMEGKYRLATLDAPREKHDHLTMKFADGQLIYADVRKFPELPEVETVRRELEKRIVGQKIISIEATYPRMVLTGFEQLKKELTGKTIQGISRRGKYLIFEIGDDFRLISHLRMEGKYRLATLDAPREKHDHLTMKFADGQLIYADVRKFGTW",
    "response#3": "MARHVFLTGPPGVGKTTLIHKASEVLKSSGVPVDGFYTEEVRQGGRRIGFDVVTLSGTRGPLSRVGLEPPPGKRECRVGQYVVDLTSFEQLALPVLRNADCSSGPGQRVCVIDEIGKMELFSQLFIQAVRQTLSTPGTIILGTIPVPKGKPLALVEEIRNRKDVKVFPELPEVETVRRELEKRIVGQKIISIEATYPRMVLTGFEQLKKELTGKTIQGISRRGKYLIFEIGDDFRLISHLRMEGKYRLATLDAPREKHDHLTMKFADGQLIYADVRKFRK"
  },
  {
    "reference": "MSTTHFGYRSVEEKDKAREVRKVFDSVAPKYDVMNDLMSAGLHRAWKAYTVMVANVQEGQQVLDIAGGTGDLALAFAKKAGPRGCVVHTDINAAMLSTGRDRLLDLGVVLPTTVCDAEQLPFADNRFDLVSVAFGLRNMTHKDVALAEMCRVLRPGGKLLVLEFSQVARPLAKAYDWYSFKVLPRIGQLVAGDASSYRYLAESIRMHPGQRELKTLMQKSGFGHVDYHNMTGGIAALHVGIKC",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in ubiquinone biosynthetic process, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "LQDRVAPFDGALARKHIELAMGGPLETWFDDFDQQALASASIAQVHTARLKTTGQEVVLKVIRPDIRPIIKADVRLMYRLAAWVPKLMPDGRRLRPREVVREYEKTLLDELNLLREAANAIQLRRNFDGSPMLYVPEVYSDYCRESVLVMERIYGIPVSDIPTLEKQGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVSYEHPEDPCYIGIDCGIVGSLNKDDKRYLAENFIAFFNRDYRKVAELA",
    "response#2": "LQDRVAPFDGALARKHIELAMGGPLETWFDDFDQQALASASIAQVHTARLKTTGQEVVLKVIRPDIRPIIKADVRLMYRLAAWVPKLMPDGRRLRPREVVREYEKTLLDELNLLREAANAIQLRRNFDGSPMLYVPEVYSDYCRESVLVMERIYGIPVSDIPTLEKQGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVSYEHPEDPCYIGIDCGIVGSLNKDDKRYLAENFIAFFNRDYRKVAELH",
    "response#3": "LQDRVAPFDGALARKHIELAMGGPLETWFDDFDQQALASASIAQVHTARLKTTGQEVVLKVIRPDIRPIIKADVRLMYRLAAWVPKLMPDGRRLRPREVVREYEKTLLDELNLLREAANAIQLRRNFDGSPMLYVPEVYSDYCRESVLVMERIYGIPVSDIPTLEKQGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVSYEHPEDPCYIGIDCGIVGSLNKDDKRYLAENFIAFFNRDYRKVAELE"
  },
  {
    "reference": "MKSKLPKVLHELNGRSMLGHVLAAARELDPQNSVVVVGHARDQVRAHLEVVAPEATTTVQEEQNGTGHAVRMAVEDLAARGTELSGTVVLTCGDTPLLRGSTLSELVAAHEKEGNALTALSARVPDPHGYGRIVRDEDGDFTEIVEQADATPRQHAIDEINSGMYAFDGALLCEVVQRLSTDNAKGEEYVTDAVALLRGDGHRVGAWVAEDWHEVQGVNNRVQLSEARRVLNDRLLAEHMLAGVTVVDPATTWVDAQVTIGRDAVLEPGTRLLGATAVGEDAVVGPRADLRDTVVGAGATVSETTADRAEIGPGATVGPYTYLRPGTRLAERTKAGAFVEVKNSNIGTGSKIPHLTYVGDADIGESSNIGCSSVFVNYDGVNKSRSVIGDHVRIGSDNTIVAPVRVGDGAYSGAGTVVRDDVPPGALAISEGHRQRNVEGWTRRKRPGTASAEAADKADRHRADEEQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).2. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.3. The presence of MobA-like NTP transferase domains is critical for the interaction of the designed protein with its intended targets.4. The protein design must be able to perform UDP-N-acetylglucosamine diphosphorylase activity, glucosamine-1-phosphate N-acetyltransferase activity in order to contribute to cell wall organization, lipid A biosynthetic process, regulation of cell shape, UDP-N-acetylglucosamine biosynthetic process.5. The protein should be optimized for activity in UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway and exhibit minimal cross-reactivity with other metabolic pathways.6. The acetyl-CoA binding site should be located in a region of the protein that is accessible to the ligand.7. The protein must bind to Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "VVILAAGQGSRMKSSLPKVLHPVAGKPMLHHVIETAMALDAEQIHVVIGHAGDRVREASDTYPVNWVEQSEQLGTGHAVAQVLPHLPDQARVLILYGDVPLTRTQTLHQLTDTLDERQLGMLEIRTDDPTGYGRIVRDAAGHVQAIVEHKDASAAQRAIREVNTGILAAPTPLLRKWLARLRNDNAQGEYYLTDVIAMAVADGGTVATAQPTRDIEVQGVNNRVQLAALERAWQRNIANALLERGVTLADPERLDVRGQVTVGQDVFIDANVLLEGDVVLEEGVRVGFGCVIRDSRIARGTEIHPYSVIESSVIGENAQIGPFARFRPGNTLAANTKVGNFVEIKKSVVGAGSKINHLSYVGDARLGERVNVGAGTITCNYDGANKHATVIGDGAFIGSNTSLVAPVTVGSDATIGAGSTITRDVADTELAVARGKQRNIAGWTRPKKK",
    "response#2": "VVILAAGMGKRMQSALPKVLHPLAGKPLLRHVIDTARSLSPQKLCVIYGHGGAAVPDMVSALAQETGVTIDTALQQPQLGTGHAVMQAVPQLDDGSATLVLYGDVPLTTADTLRRLVEAAGSDKLGILTVEQLTNPDGYGRILRTQDGDVQAIVEQKDASPEQLKICEVNSSIYCFNAEKLFAALPKLSTDNAQGELYLTDTVALLRAQGGKVVAAIAPDAFEILGCNDRAQLAELARLLRARTVQKLLEQGVMLSDPERFDLRGELQHGKDVFIDANVILEGQVTLGNRVRIGTGCVLKNAIISDDVVISPYTMIEDGRVAAHSTLGPFARLRPGSELGEGAHVGNFVEIKQARLGKGSKAGHLSYLGDAEVGAHVNIGAGTITCNYDGANKHKTVIGDDVFIGSYSQLIAPITIGHGSTIGAGTTVTHDVADGEMVISRIRGRSRIEGWKR",
    "response#3": "VVILAAGMGKRMQSALPKVLHPLAGKPLLRHVIDTARSLSPQKLCVIYGHGGAAVPDMVSALAQETGVTIDTALQQPQLGTGHAVMQAVPQLDDGSATLVLYGDVPLTTADTLRRLVEAAGSDKLGILTVEQLDDPEGLGRIVRDGSGNVCGIVEERDATEEQREIREINSGVYCVDSGVLRAHVERLSTDNAQGELYLTDVIALAREDGLTVSAHRCADTWETLGVNDRRQLSELQREMNRRILEHWMREGATLADPARFDVRGQLTLGQDVFIDVNVVIEGRVEIGSKVTIGAGCVLKDCRIGDGAVLKPYTLVEEATIGEICTAGPFARLRPGAELVHDSHIGNFVEMKKASLGRGSKANHLAYIGDAKVGEKVNIGAGTITCNYDGANKHLTVIEDNAFIGSDTQLVAPVTVGKGATVAAGSTVTKDVGEGELVLTRIKGPTVIEGDTVLGQDVVVEPHVVFAPGVKVEDGATIRAFCHLRDAEVGADAEVGNFVELKKTRIGRGSKTKNHLSYIGDAEVGADVNIGAGTITANYDGVNKHRTTVIGDDVFVGSDTQLVAPVSVPDGTTIAAGTTLVIGRDVTLDCNVIIEGRVVLGDRVTIGAGCVLKRDVPEGVVAIDRARQRNIEGWVQRKRD"
  },
  {
    "reference": "MKLLRDILAHVQALESRGPLDVMVEGISFDSRQVRAGVLFVAVQGVQSDGHDFIAKAVEAKATAIVCEVLPEQLTSDTTYVLVKDSAKALGQMASVFHDHPSEKLKLIGVTGTNGKTTSVTLLHKLFRELGYHVGLLSTVQNQIDETVLPASHTTPDAVSLNALLHQMVKAGCAYCFMEVSSHAMVQQRVAGLQFAGGVFTNITHDHLDYHKTFDEYIRAKKLFFDGLPAGAFALINSDDKRGAVMVQNTKAEVHFYSLRRATEFKAKVIDNTLQGLHLEVDGHEIWCKLIGLFNAYNLLSAYAVAVLLEEDPVESLTVLSSLNSASGRFEYIVSDTQITGIVDYAHTPDALENVLNTIQQIRNPNQQVITVVGCGGNRDAAKRPLMADLACRLSDKVVLTSDNPRFEEPQAILEEMQKGVRPIDYKKTLSVLDRREAIRTACMLASTHDIILVAGKGHETYQEVKGVKHPFDDKQVLQETFQQLGK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.2. The designed protein must have at least one Mur ligase N-terminal catalytic domain. The designed protein sequence is ",
    "response#1": "VTGTSGKTTTSYLLEAGLLHAGYSVGLIGTTGTRINREPVPTSLTTPEAPTLQELFARMLSEGVTHVVMEVSSHALELGRVRGTRFDVGGFSNLSQDHLDFHPTMEDYFEAKAALFDGPQAAERAVICVDDDWGKRMAKRAAALPLTRVATQGEADITARQLSTEATGAQQVDLTIAASGTSYEFRLPLPGEFNIANAALATGS",
    "response#2": "TGTSGKTTVTYLIESGLRASGRRTGLIGTVEMRVGDERVDSSLTTPESTDLHGLFAVMRERGVDAAAMEVSSHALAKGRVGGAHYDVAIFTNLSQDHLDFHADLRDYFDTKARLFTPEFARIAVVNRDDRFGRALIDMVAAHGEVPVTTFSSEGDPEADWRAVDVELGPDGSTFRIVGPGGLETNAAVRLPGPFNVSNAMAAIF",
    "response#3": "VTGTNGKTSCAYWLMQAFASAGIKAGSIGTLGVVYPDGRLVDSDLTTPDVVSMHRMLADLRDGGARYVLIEASSIGLEQGRLNAVRIVTAAFTNLSRDHLDYHQSMQAYEAAKGLLFDRPELKHRIINLDDEAGRRLMGRWSGRSIGYGCAKSDMQLDYFAANIRIEDNGLVFELCDDAQRIEIRTRLLGQHNVSNLLCVASA"
  },
  {
    "reference": "MTVTSQTSPSRPTRPTLAQLAELTGGRLVDVAGTAAGVASAEQGQQTVATAAIDARDVPADGLFMAVPGTRAHGARFAGQSAGHSVLTDEEGLGVLVDGSWQGTALVVDDCRQWLGPVAAEIHGHPSRDMTVIGVTGTSGKTTTTYMMERALLGHRHVGLIGTTGTRIDGRKIPTSLTTPEAPTLQALFARMRDEGVTHVVMEVSSHALVLGRVRGVDFDVAGFTNLSQDHLDFHPTMEDYFDAKSMLFTEPQDPAGKLPVPVVCVDDEWGRRLAGRTGDATVTVSTDGEAATWQVSDVAVHPDGTQHVTVDRADGQVEYDLSIPGAFNVANATLALATLVAAGEDASIADELAEVSVPGRMEKISGSSPQTAVDFLALVDYAHKPGAVTAVLDTLNGQLDRAAAGGNGATRGRLGVVLGAGGNRDHEKRPKMGAAAAAVADLVIVTDDNPRDEDPATIRAAVLAGAGDAAAQRTDGVSPEIREIGDRAEAIRDAVGWARSGDVVVVAGKGHESGQIIGDTVVDFDDRVELADALAALDVHSDTKDSEA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must bind to Mg(2+) in order to perform its enzymatic function.2. The protein should have a low Km value for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.3. We require a protein that can specifically bind to the metabolites produced in the Cell wall biogenesis pathway.4. The protein should be able to bind UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate, ATP ligand in a variety of conditions5. The protein design must feature the Meso-diaminopimelate recognition motif motif.6. A protein that can perform magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity in cytoplasm to support regulation of cell shape, cell division, cell cycle, cell wall organization. The designed protein sequence is ",
    "response#1": "MKLSDIIKNINYLEIIGSNDCNINSIDFDSRNIANNDVFVAIRGTVVDGHNYINVAIDKGALAIICETLPDKIICKKISNIKDITSKLSAYFFNYPSKKLNIIGVTGTNGKTTIATLLYNMFRKMGHKCGLLSTVCNYIEDEAIPADHTTPDPIELNMLLGRMVEAGCEYAFMECSSHAIAQKRIGGLMFAGGIFTNLTRDHLDYHKTFENYRNAKKAFFDDLPKTAFAITNADDKNGMIMVQNTKATVKTYSETKMADFRGRIIECHFGGMYLDIDGHEVGVQFIGKFNVSNLLAVYGAICYLQNLNSKDILLSLSKLKPVAGRIERLRAPDGRMVVVDYAHTPDALKNVLETLLQIRGSKPYAIIGVIGAGGDRDRTKRPKMGRIAASLCDRLIVTSDNPRSEDPEMIIEEIISGIDPSDRNKVISIVNRAEAIRAACLMAHPGDVVLIAGKGHENYQEIKGVKHHFDDKEILKNIFK",
    "response#2": "MRIHGLVQDSRMVGPGDAFVAIGGFGTHGLHFVEQARAAGAAAILFEPPAPAGLDAPADAIAVPNLRARMGVLADRFYGRPSERLALIGVTGTNGKTTVVSLIKALLDSYQKPCGLLGTIEHILGEGVIKDGFTTPTPALLQKYLATMVRQNREAVAMEVSSIGLALGRVAYTNFDAAVLTNITLDHLDFHGSFETYVEAKAKLFSFIPSSGLAVVNADSPYALPCTENTSAKIVTYGIETRADYQATDIQLSCFETKYTLIHREEKFACSSLFIGKHNVYNMLAAISTLLAMDYPLAGLVQVASRLQPVLGRMEAFSGTNKPLVIVDYAHTPDALQQVLTALRRHCHGTLWCVFGCGGDRDRGKRPQMGAVAAELADRVVVTDDNPRTEDPQQIVRDILAGMPRQRNVQVIMGREHAVQQVIAQAQPNDVVLLAGKGHEDYQEIQGVRQPFSDQEVARTVLRP",
    "response#3": "MRLRDLLAAIPGLDAGPQTDVGGVKLDSRQVAANDLFVAVPGYQVDGRQFIDAAIAAGAAVVLAEAEVPDGCVGGVKLDSRQVAANDLFVAVPGYQVDGRQFIDAAIAAGAAVVLAEAERIVLGSVAARFYGRPADRLAVIGVTGTNGKTTIATLLYNMFRKMGHKCGLLSTVCNYIEDEAIPADHTTPDPIELNMLLGRMVEAGCEYAFMECSSHAIAQKRIGGLMFAGGIFTNLTRDHLDYHKTFENYRNAKKAFFDDLPKTAFAITNADDKNGMIMVQNTKATVKTYSETKMADFRGRIIECHFGGMYLDIDGHEVGVQFIGKFNVSNLLAVYGAAGVAAGIDPARVLPGLAETTVPGRLELVGDRFGAPVFVDYAHKPDALVKALEALRPYAKGRLVVVFGAGGDRDAGKRPLMGEIAARHADTVIVTDDNPRSEDPAAIRAAILEAAPGAQNIGDRAEAIRTAVAALAPGDVLLIAGKGHEPYQEIAGVRHPFDDREVAREALAARAGG"
  },
  {
    "reference": "MALDILAMAPLYQAPAITRIGPKTDPSKRPADPLKPLVPSRTKLTTIEAKRIMSILDEAINKVELVTLLSYVASNREDVEGVLGEDIMRAVREHEEEAEEEGWLRDRLLSIELQKSSLSPLTQQIKDSTKNVLRLLLSNPQAARLLQMQTQSRSAEAQNFIDSLIELRGFLFEKLVTSPMEARDKAQFIQDINRQNSNNQQIIDTLENELAERMKKRNAEVEKENFVIQELKNHLHQVLKFSENSLLRTKQEAEKQQKADFRASQARVAKIQQEILQLQSQFYNLVMENREAEQALRKKKYKVETEIENWIQKYDTEMGEKQEELEDLEAVHKEEKIALEELKRRHKVLVEEFVQIREEREINSKKRMEAEQEMVRMVRAATLIQAFWKGYLVRSLLRSKKKRGKGKAKGKEKGKQKGKEKGKGKK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein localizes to the ciliary basal body. The designed protein sequence is ",
    "response#1": "WGYCSNTVVYAYTRPDRPEYCVVFWDTKNNEKYVKYVKSLISITTCGDFCILATKADENHPQFVLVLCNSIGTPLDPKYIDLVPLFVAMTKTHVIAASKEALYTWQYRVAKKLTALEINQITRSRKEGRERIYHVDDVPSGSVDGVFDYSKAIQGTRDPICAITASDKTLIVGRESGVIQRYSFPNVALIQKYSLDCRACQLSLNCNSSRLAIIDIAGVLTFFDLDTRVTDSTGQQVVGELLKLERKDVWDMKWAKDNPDLFAMMEKTRMYVFRNLDPEEPIQTSGYICNFEDLEIKSVLLDEILKNPEHPSKDYIMNFC",
    "response#2": "WGYCSNTVVYAYTRPDRPEYCVVFWDTKNNEKYVKYVKSLISITTCGDFCILATKADENHPQFVLVLCNSIGTPLDPKYIDLVPLFVAMTKTHVIAASKEALYTWQYRVAKKLTALEINQITRSRKEGRERIYHVDDVPSGSVDGVFDYSKAIQGTRDPICAITASDKTLIVGRESGVIQRYSFPNVALIQKYSLDCRACQLSLNCNSSRLAIIDIAGVLTFFDLDTRVTDSTGQQVVGELLKLERKDVWDMKWAKDNPDLFAMMEKTRMYVFRNLDPEEPIQTSGYICNFEDLEIKSVLLDEILKNPEHPSKDYIMNFC",
    "response#3": "WGYCSNTVVYAYTRPDRPEYCVVFWDTKNNEKYVKYVKSLISITTCGDFCILATKADENHPQFVLVLCNSIGTPLDPKYIDLVPLFVAMTKTHVIAASKEALYTWQYRVAKKLTALEINQITRSRKEGRERIYHVDDVPSGSVDGVFDYSKAIQGTRDPICAITASDKTLIVGRESGVIQRYSFPNVALIQKYSLDCRACQLSLNCNSSRLAIIDIAGVLTFFDLDTRVTDSTGQQVVGELLKLERKDVWDMKWAKDNPDLFAMMEKTRMYVFRNLDPEEPIQTSGYICNFEDLEIKSVLLDEILKNPEHPSKDYIMNFC"
  },
  {
    "reference": "MSKIWILLLLVGAVQFARGFPALEEEQEDDVIDWPSFEYDLSDEERGKGLDWLKKQWGKLKNSFKKVGAKVTATFNKGRDYLKKKGIKVDPLNCQGNKCRSCVIFTLKPKKFCIEYVFSTSAITVSLIKEKDDEEKVLLGPFTIKTGNIPKCCKLGSFIGELCLQGVEGRLKSSNGKPHVNLCVGLLLKKFGCGAKICVSYVDGKFSVSFKPKLFAGDEENGTIMEAGEKEDEGKVIDAVPE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein can be targeted to extracellular region and perform toxin activity.2. Design the protein with a signal peptide incorporated.3. The protein must exhibit the following characteristics: Highly abundant protein that may be responsible for the observed disruption of sensory neuron membranes, since it is homologous to proteins such as trialysin, which forms pores in lipid bilayers (Probable). The designed protein sequence is ",
    "response#1": "RDAYIAKNYNCVYECFRDAYCNELCTKNGASSGYCQWAGKYGNACWCYALPDNVPIRVPGKCK",
    "response#2": "SWSTTSGR",
    "response#3": "GCCSGTCNKTFGVC"
  },
  {
    "reference": "MGGXLSKPRFXGWATIRERIRRTQPXAEGVGAVSRDLEQHGAITSSNINNAACXWLEAQEQEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQXILDLWVYHTQGYFPDWHNYTPGPGTRXPLTFGWCFKLVPVDPDRVEEITEGENNCLLHPINQHGMDDPEREVLXWRFDSRLAFHHVAREXHPEFYKDC",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that contributes to suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II by functioning in extracellular region with SH3 domain binding.2. The designed protein should contain at least one Dileucine internalization motif; necessary for CD4 internalization motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "MLPEL",
    "response#2": "MPSGGMR",
    "response#3": "GAPEXRXF"
  },
  {
    "reference": "MASPLPLLYLCLAALHLAGARDATPTEEHTSTARGLQGRPPDTGQPSPALEDWEEASEWTSWFNVDHPGGDGDFESLAAIRFYYGPARVCPRPLALEARTTDWALPAAMGERVHANPERGFWCLNREQPRGRRCSNYHVRFRCPLEAAWGAWGAWGLCSKSCGLGRRLRRRSCQSSSGDTCPGSPQEAQKCVRSRCPGCSSDTCGCPNHILLGSVVTPSGRPLSGARVSLRTRPGTIATSGTHGTFQVPGVCAGSKASVSAQMNGFSAGTAQAHANSSNTATVTIILEELGKPYLVKHPESRVREAGQNVTFCCKASGTPMPKKYSWFHNGTLLDRRQQGSGPHLELQGLHQAQAGEYHCKAWNEAGTVRSRAALLTILAPGQQACDPRPQEHLIKLPDDCGQPGGGPTYLDVGLCADTRCPGPVGSGPRCGDAGSRCCSVLRLESRDIRCSGYVLPVKVVAECGCRKCLPRRGLVRGRVVAADSGEPLRFARILLGRAPIGFTSYQGDFTIEVPPATERLVVTFVDPSGDFVDSVRVLPFDPRGAGVYHEIRALRKAAAVLLDAERGGEIPLGSTEEAPALGELVLPPGTFHHPDGRPYTGPVEARVTFVDPRDLASASAASSDLRFLDSAGELAPLRTYGMFAVDLRAPGSTEQLHVARADVHVDADHVRMPGHAEALALWSLDPETGLWEEEGSEQGSGGFRRETAAARVRREERAFLVGALTMRERRLFNLDVPERRRCFVKVRAYGTDRFAPAEQVQGVVVTL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Design the protein with a signal peptide incorporated.2. The protein can be expressed in extracellular space and perform carbohydrate binding without triggering undesirable cellular responses.3. For general function, the protein need meet that May play a role in cartilage scaffolding. The designed protein sequence is ",
    "response#1": "RAYQTVVLDPEGDAQIDPNWVVLNQGREIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTVTDDDYAGFIFGYQDSSSFYVVMWKQMEQTYWQANPFRAVAEPGIQLKAVKSSTGPGEQLRNALWHTGDTESQVRLLWKDPRNVGWKDKMSYRWFLQHRPQVGYIRVRFYEGPELVADSNVVLDTTMRGGRLGVFCFSQENIIWANLRYRCNDTIPC",
    "response#2": "REDFRFCSQRNQTHRSSLHYKPTPDLRISIENSEEALTVHAPFPAAHPASRSFPDPRGLYHFCLYWNRHAGRLHLLYGKRDFLLSDKASSLLCFQHQEESLAQGPPLLATSVTSWWSPQNISLPSAASFTFSFF",
    "response#3": "LSLLSYVGCVVSALACLVTIAAYLCSRVPLPCRRKPRDYTIKVHMNLLLAVFLLDTSFLLSEPVALTGSEAGCRASAIFLHFSLLTCLSWMGLEGYNLYRLVVEVFGTYVPGYLLKLSAMGWGFPIFLVTLVALVDVDNYGPIILAVHRTPEGVIYPSMCWIRDSLVSYITNLGLFSLVFLFNMAMLATMVVQILRLRPHTQKWSHVLTLLGLSLVLGLPWALIFFSFASGTFQLVVLYLFSIITSFQGFLIFIWYWSMRNAEVRKRWTR"
  },
  {
    "reference": "MDLIVATQNENKVLEFKKMLGDKFLIKSLNDLNFKDEIPENENTIRKNAVYKSNFIYKIFNCNVFSDDTGLEIEALNNEPGVKSARYSGENRDTKKNIELVLKKMEGLIKRKARFRTVISLIYYGKEYCFEGIVNGNITETPIGDNGFGYDSIFRPNGYKKTFAQLSMNKKNKISHRALAINKLIDFLKKN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The Proton acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "VTPEVIKKFKKKYEPTALRVKVLVSIGNKNKQFPFTIGSDSNSEAWVSEATASLKSIIKTYNLDGIDVSYEDIAANEADFVNSVGGLVRNLKQNKLITVASFATSADAANNKFYNC",
    "response#2": "VTPEVIKKFKKKYEPTALRVKVLVSIGNKNKQFPFTIGSDSNSEAWVSEATASLKSIIKTYNLDGIDVSYEDIAANEADFVNSVGGLVRNLKQNKLITVASFATSADAANNKFYNN",
    "response#3": "VTPEVIKKFKKKYEPTALRVKVLVSIGNKNKQFPFTIGSDSNSEAWVSEATASLKSIIKTYNLDGIDVSYEDIAANEADFVNSVGGLVRNLKQNKLITVASFATSADAANNKFYNC"
  },
  {
    "reference": "MLPNTGKLAGCTLFITGASRGIGKAIALKAARDGANVVIAAKTAEAHPKLPGTIYTAASEIEAAGGKALPCIVDVRDENQISAAVEKAVDTFGGIDILVNNASAISLTNTLETPMKKVDLMMGINTRGTYLTSKICIPYLKKSKVAHILNLSPPLNLNPIWFKNHCAYTIAKYGMSMCALGMSEEYKGEIAVNALWPKTAIHTAAMDMLGGSGVDKQCRTPDIMADAAYAILSKPKDFTGNFVIDEELLKHEGIKDLDVYAVSPGHPLLPDFFLDESPEALASAMEEHGATAAFKAGKAQAKSQDSSPLQETFKAIESLVNEEAVKTTQGIYQFVLSGEESGNWFLDLKNGKGGVGSGEPSTKADVVMSMDSGDFIKMFTGKMKPTMAFMSGKLKIKGDMGLALKLEKILGQMNAKL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be able to compete with endogenous proteins or inhibitors for NADP(+) binding. The designed protein sequence is ",
    "response#1": "LLPRRQYSAYKNQFGRIGVVAGSKGFIGAALMTSQGALRAGAGLVEVFVPEEIYEIVASTAPMEAMVKPVQSYRDLLKQKTDVWALGPGLGKSRAGEVLELIEKAKQPMVTDADGLNILSEKNSVLRRCKGERLLTPHPGEMKRLFPDHKEPRAETATKFCNRFPVTLLFKGSRTIVAERDRPLTYNTTGNPGMATGGMGDILTGVCAGLIGQGLSLYDAARVGAWMCGRAAEIAIFNDGQSEQSLLPRDVLDHGKQEVLPIYKPRDKFSHKGNFGHAVIIGGSYGKIGAVNLASRGALSSGAGLITTYTPKCGYIPLQSNFPEAMVLTDDEETFISNMNIGFEASAIGIGVGMGTHKKTVSALEAFLKQNKLPLVVDADGLNCISKNKDLISLLPENTVLTPHPKELERLIGNWDDDFDKLSKVKAFSAKHKIIVVLKGANTITVMGDKLYVNTTGNPGLATAGTGDVLTGVITGLIAQGYDPLAASVFGVYLHGKAADIAVEDFGYQSLIASHVIEALGQAYIDLFR",
    "response#2": "GVVAGSKGFIGAALMTSQGALRAGAGLVEVFVPEEIYEIVASTAPMEAMVKPVQSYRDLLKQKTDVWALGPGLGKSRAGEVLELIEKAKQPMVTDADGLNILSEKNSVLRRCKGERLLTPHPGEMKRLFPDHKEPRAETATKFCNRFPVTLLFKGSRTIVAERDRPLTYNTTGNPGMATGGMGDILTGVCAGLIGQGLSLYDAARVGAWMCGRAAEIAIFNDC",
    "response#3": "SPPAFHGLLPRRQYSAYKNQFGRIGVVAGSKGFIGAALMTSQGALRAGAGLVEVFVPEEIYEIVASTAPMEAMVKPVQSYRDLLKQKTDVWALGPGLGKSRAGEVLELIEKAKQPMVTDADGLNILSEKNSVLRRCKGERLLTPHPGEMKRLFPDHKEPRAETATKFCNRFPVTLLFKGSRTIVAERDRPLTYNTTGNPGMATGGMGDILTGVCAGLIGQGLSLYDAARVGAWMCGRAAEIAIFNDGQSEQSLLPRDVLDHLGDAFNED"
  },
  {
    "reference": "MAQVWKYYEIKDGKLIRKKKVCPRCGSFMAEHKDRYHCGKCGYTEWKVERPKIVIHGATIE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects.2. The protein can be expressed in ribonucleoprotein complex and perform zinc ion binding, structural constituent of ribosome without triggering undesirable cellular responses.3. The presence of Zn(2+) is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "DTHTHTYASGHAYSTLIENAKSAKQNGLAMFCTTDHSESMPGAPHYWFFSNQRVLPRFIEDVAIIRGVESNIMNTQGEIDIHPSVDKNLDWVIASFHEPVFRPSDVATHTEALLNVIKGGRVDALGHLGNY",
    "response#2": "DHRLLAKKMDLFHFQPESPGNVFWHPNGWSIILQMREYIRHITHKYGYQEVHTPQLIDASLWDKSGHLEKFGDDIFSLPLEPQQYVIKPMSCPAHVQIFNQGVKSYRDLPLRYAEFGACHRNEPSGTLHGLMRLRGFVQDDAHIFCTEDQIQSEVSAFIDQLHEVYADFGFTEVIHKLSTRPEKRVGSDEVWTKAEQALAEALNRKGVEWDILPGEGAFYGPKIEFSLRDCLGRIWQCGTVQVDFSVPERLGAHYIAEDGSKKPPVMIHRAILGSFERFLGILLEESAGKLH",
    "response#3": "SLAGSYLVIMPNNPRAGGISRRIEGDERLELKEALSSLEVPDGVGLIVRTAGVGKSSEELQWDLKVLLHHWEAIKQVSESRSAPFLIHQESDVIVRAIRDYLRRDIGEILVDSPKVYEKAKAHIKLVRPDFINRIKLYQGEVPLFSHYQIESQIESAFQREVRLPSGGSIVIDATEALIAIDINSARSTRGGDIEETALNTNLEAADEIARQLRLRDLGGLVVIDFIDMTPVRHQREVENRMREAVRQDRARIQISRISRFGLLEMSRQM"
  },
  {
    "reference": "MAIDLKKHKSRRIILTKQGKRTERHIPVLLQPVLAGLIPLVGAKVIDGTFGAGGYTRALLNAGAQVIALDRDPHAIREGQSLVDEFFPRLRLVQGNFSQLDCVVEEKVDAVILDIGVSSMQLDEAERGFSFQKDGPLDMRMAQTGFTAADVVNRLKSDELARIFKILGEERYARRIARMIEKRRCVQPFLRTGDLAHAIEVLVGRKSGERIHPATRVFQALRIYVNDEIGELARGLFAAERVLKPGGRLGVVSFHSLEDRMVKRFFSARSGESVRSRYLPEIEMAPATFFPLFKGGITANKEELERNPRSRSARLRIGVRTEAECLFADMKLFGLAKIASFEGGKK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be expressed specifically in the cytoplasm2. The protein must exhibit the following characteristics: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA.. The designed protein sequence is ",
    "response#1": "MIYLVGTPLGNLEDITLRAIRVLGEADVVACESKERALKLLSHLQIKKPLIYLREASRASDVKRIIELHKQGKTVAVTTDAGMPGISDPGAYLVRELSAQGIPFTVVPGPSALCMSITVSGLEEPWAFLGFLPLKEGKRRRELEKFLPLNIGLVIYEGPHRVGKLLSLLNELCPQRKVALLRELTKLHEEVQR",
    "response#2": "MIYLVGTPLGNLEDITLRAIRVLGEADVVACESKERALKLLSHLQIKKPLIYLREASRASDVKRIIELHKQGKTVAVTTDAGMPGISDPGAYLVRELSAQGIPFTVVPGPSALCMSITVSGLEEPWAFLGFLPLKEGKRRRELEKFLPLNIGLVIYEGPHRVGKLLSLLNELCPQRKVALLRELTKLHEEVQS",
    "response#3": "LTIKTLPGVFSRDGLDVGSDLLLSTLSPHTKGKVLDVGCGAGVLAAVLASHSPKVRLTLCDVSAPAVEASRATLAANGFEGEVVASNVFSEIKGRFDMIISNPPFHDGMETSFEAAQTLIRSAVRHLNIGGELRIVANAFLPYPNVLDETFGNHEVLAQTGRFKVYS"
  },
  {
    "reference": "MRRLRRLVHLVLLCPFSKGLQGRLPGLRVKYVLLVWLGIFVGSWMVYVHYSSYSELCRGHVCQVVICDQYQKGIISGSVCQDLCELQKVEWRTCLSSAPGQQVYSGLWQDKEVTIKCGIEEALNSKAWPDAVPRRELVLFDKPTRGTSIKEFREMTLSFLKANLGDLPSLPALVDQILLMADFNKDSRVSLAEAKSVWALLQRNEFLLLLSLQEKEHASRLLGYCGDLYLTESIPHGSWHGAVLLPALRPLLPSVLHRALQQWFGPAWPWRAKIAIGLLEFVEELFHGSYGTFYMCETTLANVGYTATYDFKMADLQQVAPEATVRRFLQGRHCEQSSDCIYGRDCRAPCDKLMRQCKGDLIQPNLAKVCELLRDYLLPGAPADLYEELGKQLRTCTTLSGLASQVEAHHSLVLSHLKTLLWREISNTNYS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein's functional design necessitates the inclusion of a May mediate ER retention motif.2. The designed protein should be expressed specifically in the endoplasmic reticulum membrane3. The Lumenal topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.4. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "VWALLQRNEFLLLLSLQEKEHASRLLGYCGDLYLTEGVPHGAWHAAALPPLLRPLLPPALQGALQQWLGPAWPWRAKIAIGLLEFVEELFHGSYGTFYMCETTLANVGYTATYDFKMADLQQVAPEATVRRFLQGRRCEHSTDCTYGRDCRAPCDRLMRQCKGDLIQPNLAKVCALLRGYLLPGAPADLREELGTQLRTCTR",
    "response#2": "VWALLQRNEFLLLLSLQEKEHASRLLGYCGDLYLTEGVPHGAWHAAALPPLLRPLLPPALQGALQQWLGPAWPWRAKIAIGLLEFVEELFHGSYGTFYMCETTLANVGYTATYDFKMADLQQVAPEATVRRFLQGRRCEHSTDCTYGRDCRAPCDRLMRQCKGDLIQPNLAKVCALLRGYLLPGAPADLREELGTQLRTCTG",
    "response#3": "VWALLQRNEFLLLLSLQEKEHASRLLGYCGDLYLTEGVPHGAWHAAALPPLLRPLLPPALQGALQQWLGPAWPWRAKIAIGLLEFVEELFHGSYGTFYMCETTLANVGYTATYDFKMADLQQVAPEATVRRFLQGRRCEHSTDCTYGRDCRAPCDRLMRQCKGDLIQPNLAKVCALLRGYLLPGAPADLREELGTQLRTCTC"
  },
  {
    "reference": "MTIADRWLLPDGMDEVLPPQATRMEQLRRALLDLYDRWGYDLVIPPTVEFLDSLLTGTGTDLDLQTFKLTDQLSGRMMGASADVTPQVARMDAHSLKRSGPARLCYCTTVLRAKADKHQGGRSPTQVGVELFGHAGLDADIEVVRLALTGLEVAGAGEVHLALGHIGIYRALVHAAALSAESEQALFEAIERKAFNDVDALVARDVSDPALVDMLQALPRLYGGQEVLDQAREVFAGAPPAVMAALDELQALCRAVTDNHLRAEVYLDLAELRGYLYHTGMVFAAYVPGYGQALAKGGRYDDTGRAFGRARPATGFSMDLKLLASLEESGPRCDGIWAPADERAGLEDAIARLRASGERVIQALPGQRVGPREQRCDRQLVESNGEWRVEPLA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Required for the first step of histidine biosynthesis.2. The protein design should take into account the physiological conditions of the L-histidine biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MLAYMNSEALEKTIDTGYTWFYSRSRAALWNKGETSGNTQKVVSVTADCDNDTLLVRVKSNGPACHTGNRTCFL",
    "response#2": "MLAYMNSEALEKTIDTGYTWFYSRSRAALWNKGETSGNTQKVVSVTADCDNDTLLVRVKSNGPACHTGNRTCFL",
    "response#3": "MLAYMNSEALEKTIDTGYTWFYSRSRAALWNKGETSGNTQKVVSVTADCDNDTLLVRVKSNGPACHTGNRTCFG"
  },
  {
    "reference": "MRVREIKRNYQHLWRWGTMLLGMLMICSVTGKLWVTVYYGVPVWKEATTTLFCASDAKSYKAEAHNIWATHACVPTDPNPREIILENVTENLNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCLEAKVWNATGQNTTVNSTIDMGMKNCSFNVTTEVRDKKKQVRALFYKLDVVQIDDTNTSSYRLINCNTSAITQACPKVTFEPIPIHYCAPAGFAILKCNDKKFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTDNAKIIIVQLNESVTITCTRPYSNKRQSTHIGPGRAYYTTDIIGDIRQASCTVNESAWNRTLQKVAGQLEKYFRNKTIIFANSSGGDLEITTHSFNCGGEFFYCNTSGLFNSTWNHNASTQESTGNITLPCRIKQIINMWQGVGRAMYAPPIEGLIRCSSNITGLLLTRDGGDNNSANEIFRPGGGDMRDNWRSELYKYKVVRIEPLGLAPTRAKRRVVEREKRAIGLGAMFLGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVESYLKDQQLLGIWGCSGKHICTTNVPWNSSWSNKSLEEIWNNMTWREWEREIDNYTGLIYSLIEESQTQQEKNEQELLQLDQWANLWNWFSITKWLWYIKIFIMIVGGLICLRIVFAVLSLVNRVRQGYSPLSFQTLLPAPRGPDRPEGIEEEGGEQGRGRSIRLVNGFSALIWDDLRNLCLFSYHRLRDLILI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have a specific function in positive regulation of plasma membrane raft polarization, virion attachment to host cell, positive regulation of receptor clustering, mitigation of host antiviral defense response and should be localized to virion membrane in order to carry out this function efficiently.2. For general function, the protein need meet that Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Transmembrane protein gp41: Acts as a class I viral fusion protein; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane3. The presence of Retroviral envelope protein GP41-like, Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWKDADTTLFCASDAKAYKTEVHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVEQMHADIISLWDQSLKPCVKLTPLCVTLNCIDYKHNATSNSTTDISNITTDISNITTDMKNCSYNITTELWDKKKKEASLFYRLDIVPIGGNNSSEYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCNEKEFNGTGPCNNVSTVQCTHGIRPVVSTQLLLNGSLAEGEKVMIRSENITNNAKNILVQLTAPVKINCTRYNNNTRKSVRIGPGQALYTTGAIIGDIRQTHCNVSRAEWHSTLQKVVDRLKTHFKNKTIKFNSPSGGDLEITTHSFNCGGEFFYCNTSGLFNNNWDNNTRNSTDSTESNDNITLQCRIKQIINMWQRAGRAIYAPPIKGRITCESNITGLMLTRDGGDNNSTEEVFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVEREKR",
    "response#2": "LWVTVYYGVPVWKEANTTLFCASDAKAYDTEAHNVWATHACVPTDPNPQEVPLENVTEEFNMWKNDMVNQMQEDIINLWDQSLKPCVKLTPLCVTLNCTDVGNTTNGNTTNVNSTTQGTMEKGEIKNCSFNITTRVRSKVQKEYALFYKLDVVPIDNSTGKYRLIHCNTSIITQACPKVTFEPIPIHYCAPAGFAILKCRDKKFNGTGACKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVVRSANFTDNAKTIIVQLNESVVINCTRPNNXTRRSLSVGPGRAIYATGDIIGDIRQAYCNVSRTAWNKTLNQIAIKLREQFKNKTIVFNHSSGGDPEIVMHTFNCRGEFFYCNTTKLFNSTWEVNNTWHVNDSIGGDENIILPCRIKQIVNMWQEVGKAMYAPPIAGQIRCDSNITGLLLTRDGGNTNNDNTETFRPGGGNMKDNWRSELYKYKVVQIEPLGVAPTKARRRVVQREKR",
    "response#3": "LWVTVYYGVPVWKEADTTLFCASDAKAYETEVHNVWATHACVPIDPNPQEVVLGNVTENFNMWKNGMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTKLNNTATTNSSSGNITNTNNSSETVMKESEMTNCSFNVTTNIRNKVQKEYALFDKLDVVPIDENITNYMLIHCNSSSITQACPKVSFEPIPIHYCTPAGFAILKCNDKKFNGSGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVIRSENFTDNAKTIIVQLNASVEINCIRPNNNTRKGISIGPGRTFYATRAITGDIRRAHCNISEEKWNNTLRQLVIKLKEQFGKNKTIVFNQSSGGDPEIVMHSFNCGGEFFYCNTTKLFNSTWNITEGFNNITEGSNNNTITLPCRIKQIVNRWQEVGKAMYAPPIEGLIRCSSNITGLLLTRDGGNENNETNATETFRPWGGNMKDNWRSELYKYKVVKIEPLGIAPTEARRRVVQREKRALLGAVFLGFLGVAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGMWGCSGKLICTTAVPWNSSWSEKNQTEIWDSLTWMQWDREISNYTDTIYRLLEDSQNQQESNEKDLLALDSWNNLWNWFNITNWLWYIKIFIMIVGGLIGLRIIFAVLALVNRVRQGYSPLSLQTLLPAPRGPDRPEGIEEEGGERDRDRSVRLVNGFSALIWDDLRNLCLFSYHRLRD"
  },
  {
    "reference": "MARILEGVRGGPTPALVGPSDVLVSDVTLDSRQVRDGSLFCCVRGERSDGHDFAAAAVEAGATALLVERRLPLDVPQIVVADSRAATGWVAAAAHDHPSDSLMMIGVTGTNGKTTVSQLVGHVLRTTGRPTEVSGTLSGRFTTPEAPDLQRALAAARDDGVAAVSMEVSSHALALERVAGTHFDVAIFTNLGRDHLDLHGTMERYFAAKARLFEPQLSDVGVVNTDDVHGRLLFDTATISLVPFSIDDASDVEVGPTWHRYTWRGHRVEVGIGGDFNVMNTLAAATACAVAGLGEAEVAAALSLAAPVPGRFEPVQVGQDFSVLVDYAHTPDGLEQSLIAARRAAGSGRVIVVFGCGGDRDREKRPLMGAVAARLADRVVVTSDNPRSEDPMSIVDATVAGVPDDYRGSVVTEPDRRTAIGVALQLARPGDVVLIAGKGHETTQTIGDRVLPFDDRAVARELLAELGAGGGHP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP, meso-2,6-diaminoheptanedioate, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding.2. The protein design should include a Meso-diaminopimelate recognition motif motif to ensure proper function.3. The designed protein should interact with enzymes and substrates within the Cell wall biogenesis pathway to enhance or regulate its activity.4. The ideal protein would be one that plays a critical role in peptidoglycan biosynthetic process, regulation of cell shape, cell cycle through tetrahydrofolylpolyglutamate synthase activity within cytoplasm.5. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.6. The designed protein must have at least one Mur ligase N-terminal catalytic, Mur ligase C-terminal domain. The designed protein sequence is ",
    "response#1": "MKLRKISSIIIDSRRVLPGSMFVALRGTQTDGHQFIETTVNAGASAVLCEALPEKLNDKVTYIQTEDSAAAMGMIAAAFYGYPSEKLNIIGVTGTNGKTTIATMLFRLFTRLGHICGLLSTVQNQIGENIIPATHTTPDPINLNALLADMVTRGCDYAFMEVSSHAVHQQRIAGLKFAGGIFSNITHDHLDYHKTFDEYIRVKKAFFDGLPATAFALTNLDDKRGNVMLQNTRAHKATYSLRTMADFKGKILENNLTGLIMLVGEREVHFRLIGEFNAYNLMAVYGAASVLLGEEEEEILAALSELEGAEGRLQRVPAVGGAGVEVVVDYAHTPDALSQVLRALRPLATARGGRLWCVFGCGGNRDATKRPLMGGIAAAAADRVIVTSDNPRFEEPQAIVDQVLAGAADAAHVSALVDRRAAVAQAVAEAGPHDVVLLAGKGHETYQEIKGVRYPFDDRKVAADLLRMLGYDRKED",
    "response#2": "MKVSALVFDSRQVIKDAAFFAIKGTLADGHTYIAATVSAGAGVVICEVLPAEIAPDVTYIQVANSAVALGRMAANFYGNPSAQLQLVGVTGTNGKTTIATMLFRLFTRLGHICGLLSTVQNQIGENIIPATHTTPDPINLNALLADMVTRGCDYAFMEVSSHAVHQQRIAGLKFAGGIFSNITHDHLDYHKTFDEYIRVKKAFFDGLPATAFALTNLDDKRGNVMLQNTRAHKATYSLRTMADFKGKILENNLTGLIMLVGEREVHFRLIGEFNAYNLMAVYGAVLVQLGLPFSQIVQGLNQVPAVPGRFEQFHSADNIVGIVDYAHTPDALENVLETITHLRHGNEKVITVVGCGGNRDAEKRPKMAAIACKYSNLVILTSDNPRYEDPLDILEQMRKGVPAQDYKKTIVIPDRREAIFKAGLEANPEDIILIAGKGHENYQIIGTTKHHFDDREVVRELLGLE",
    "response#3": "MKLREILGQLEYIGISGSLELRCDSRAVQPGDAFIAWPGQATDGRKFVAQALAAGAAACLVEAEPEAWQTGLPQLSTPDLRKWAGLMSSRFYDHPTKQLWVCGITGTNGKTTTSHLLEAVLKENAVQTGLIGTMYTKINEEIFETKNTTPESVTIQKIFRDMKDQDVDSCIMEVSSHALDLGRVRGCDFNTVVFTNLTKDHLDYHETMDKYRQAKSLLFSQLGNSYEGEEKTAVLNADDSASLFFEKVCPAPVLTYGIDEPADIRAENISMTPTGTSFTIVTPWGEESLSLSMAGKFSIYNALAAATAAYAAGVPLESIKAGLEKAEGVPGRLQRVPAVGGAGVEVVVDYAHTPDALSQVLRALRPLATARGGRLWCVFGCGGNRDATKRPLMGGIAAAAADRVIVTSDNPRFEEPQAIVDQVLAGAADAAHVSALVDRRAAVAQAVAEAGPHDVVLLAGKGHEDYQIIGTEKHHFDDREVARAALEERE"
  },
  {
    "reference": "MLKLIIPSIMLIPMTFLINKKSLLWTATTFFSFLIAALSTLTLNMDVAEHNSTNPLLSIDQFSCPLIMLSCWLLPLTIMGSQAHMKTEPITRQKTMISLLILLQVLLCITFGASNLLMFYIAFETTLIPTLLIITRWGNQKERLTAGLYFLFYTLSASLPLLLALIMIQTHLNSLSIYIIPLSNLTLLLNTPWSETLWWIACFLAFLIKMPLYIFHLWLPKAHVEAPIAGSMILAAILLKLGGYGMIRMSSLFIPLTKDLAVPFMIIAMWGMIVTSSICLRQTDLKSMIAYSSVSHMGLVVAGIFTMTPWAWSGALAMMIAHGLVSSGLLCLANITYERTHTRSIFMNRGLKTLFPLMSFWWLMMTFANMALPPFPNFMAEILIITSLFNWSNWTILLLGLSMTLTALFSLNMLIMTQHEHPNKHAPVNPSTTREHLLMLMHMAPIILLIANPSAIMI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. For general function, the protein need meet that The immediate electron acceptor for the enzyme is believed to be ubiquinone .; Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis3. The designed protein should be expressed specifically in the mitochondrial membrane, respirasome The designed protein sequence is ",
    "response#1": "FAAPTITKLIPILFSTSGASLAYNVNLVADQFQRAFQTSTFCNRLYSFFNKRWFFDQVLNDFLVRSFLRFGYSVSFEALDKGAIEILGPYGISYTFRRLAERISQLQSGSVYHYAFAMLF",
    "response#2": "FAAPTITKLIPILFSTSGASLAYNVNLVADQFQRAFQTSTFCNRLYSFFNKRWFFDQVLNDFLVRSFLRFGYSVSFEALDKGAIEILGPYGISYTFRRLAERISQLQSGSVYHYAFAMLL",
    "response#3": "FAAPTITKLIPILFSTSGASLAYNVNLVADQFQRAFQTSTFCNRLYSFFNKRWFFDQVLNDFLVRSFLRFGYSVSFEALDKGAIEILGPYGISYTFRRLAERISQLQSGSVYHYAFAMLF"
  },
  {
    "reference": "MSELVNVAILGATGAVGAELLQLLEERDFPVKTLKLLASPRSAGKTLTFKGQEITVEEVSAESFKGVDILLASAGGATSKKWAAIAVEAGAVVVDNSSAFRMDPEVPLIVPEINPEAAKDHKGIIANPNCTTILMGVAIYPLHKVQPIKRIVVSTYQSASGAGARAMEEVKHQTEAILAGDAPKTESFPYPLAFNLFPHNSPLDEQGYCQEEMKMVNETRKIFGEPDLKVSATCVRVPVLRAHSEAINLEFEEPFEPPAAKEILSTAPGVTIVEDWEQNYFPMPIDATGKDPVLVGRIRKDISQDHGLELWLCGDQIRKGAALNAVQIAELLCEQGLLQSNTVAAAV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Proton acceptor, Acyl-thioester intermediate of the protein must be engineered to enable effective catalysis of L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.2. The protein design must be able to perform NAD binding, NADP binding, protein dimerization activity in order to contribute to 'de novo' L-methionine biosynthetic process, lysine biosynthetic process via diaminopimelate, isoleucine biosynthetic process, threonine biosynthetic process, diaminopimelate biosynthetic process.3. The designed protein should interact with enzymes and substrates within the L-threonine from L-aspartate: step 2/5, L-threonine biosynthesis pathway to enhance or regulate its activity.4. The protein should be able to bind phosphate, substrate ligand in a variety of conditions5. The presence of Semialdehyde dehydrogenase NAD-binding domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MKENIGVVGATGQVGAVVRRLLEERDFPVASIRYFASARSAGTTLPWKGEDIVVEDAAVADPTGLDVAIFSAGATLSRAQSPRFAAAGVTVIDNSSAWRMDPEVPLVVSEVNPEAIAQGDMIANPNCTTMAAMPVLKPLMELGLKKVTVATMQALSGAGFPGVPSLSITDNIIPFIKGEEEKVETEPLKILGRFDGEKIVPANIKISASCHRVPVIDGHLEAVWVEFDRSVEVDDVIKAFESLKPLDLPTSPEKVIVVRREVDRPQPRLDRDTGKGMSVVVGRIKKVEDNVIRYLVLGHNTIKKGAALNAVQIAELLVEKYL",
    "response#2": "MNIAVVGATGAVGQVVLSILEQRNFPVGELRLLASKRSAGTRISVMGEELVVDEAMPDSFDGMDIVFFAAGGSTSQALAPEAAKRGAVVVDKSSAWRMDPTVPLVIPEINPDDIREAKLHQGIIANPNCTTILMAVAVWKPLHDAARVRRVVVSTYQAVSGAGLAASQELRQATAGWLAGGAEPRQIFPHPIAANLIPQIGSPRPNGFTSEEMKMVLETRKILGDESIQVSATCIRVPVANGHSESILIETETKLSADKARHLLAGFPGVVVEDDLGAKHYPQPRTASGRDEVFVGRIREDISCPNGIALWCVSDNLRKGAALNAVQIAELLVKKS",
    "response#3": "MKIAVAGATGNVGREMLNILEERGFPADEVVPLASRRSVGQEVSYGDRTLKVQALENYDFSDTDLCLMSAGGTVSKEWSPKIGKAGCVVIDNSSAWRYDQDVPLIVPEVNPHHIDDFNKNNGIIANPNCSTIQMMVALKVLHAEAGLERLIVSTYQAVSGSGLAGAEELASQARAAVADDDLLKLVHDGSAVDMPAPVKYVRPIAFDVVPLAGSIVDDGSYETDEEQKLRNESRKILGLPDLLVSGTCVRVPVFTGHSLSVNAEFARPLSVERATELLSDAPGVKLTDVPTPLEAAGQDPSYVGRLRQDPGAPDGRGLALFISNDNLRKGAATNSIQIAELLVNKYF"
  },
  {
    "reference": "MVATNRCCVFALLFALLLVHSLTEAGKGKEILGKIKEKIIEAKDKMKAGWERLTSQSEYACPAIDKFCEDHCAAKKAVGKCDDFKCNCIKL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be able to regulate hemolysis in another organism.2. The designed protein must have at least one BetaSPN-type CS-alpha/beta domain. The designed protein sequence is ",
    "response#1": "LLKKHGCQHPIMIAGKDNSNSPTGLRQLGFKSVFAQAPIFHLSGELSIIRKEMEIKVILQNEKPDGIFLSDDMTAILTMKIANQLNITIPHELKIIGYDGTHFVENYYPYLTTIRQPIKDIAHLLVDVLLKKIDHQDTPKDYILPVGLLSGESC",
    "response#2": "LLKKHGCQHPIMIAGKDNSNSPTGLRQLGFKSVFAQAPIFHLSGELSIIRKEMEIKVILQNEKPDGIFLSDDMTAILTMKIANQLNITIPHELKIIGYDGTHFVENYYPYLTTIRQPIKDIAHLLVDVLLKKIDHQDTPKDYILPVGLLSGESC",
    "response#3": "LLKKHGCQHPIMIAGKDNSNSPTGLRQLGFKSVFAQAPIFHLSGELSIIRKEMEIKVILQNEKPDGIFLSDDMTAILTMKIANQLNITIPHELKIIGYDGTHFVENYYPYLTTIRQPIKDIAHLLVDVLLKKIDHQDTPKDYILPVGLLSGESC"
  },
  {
    "reference": "MDLFRKPKKRNAPVVRKKESSSDEDQDSEVKDVIQKRRRTNPMVQSTKQLDASTRRADNSSDDSDDSDDNQDIAVATHSFAASGDAGPSGPRDQGATATLEVDTDYSHDAQAQFERVQQQLKEGVEKDGKILYKGSALYGAKEAKDTAKGNAASGYNRVGPVRAPQFLRQTVRWDFAPDICKDYKETGFCTFGDSCKFVHDRSDYKHGWEIDEEYEAGKYGAEDDANYEIHEGDDTFPEDCFICGNPFVDPIVTKCKHYFCTGCALKSFQKSSKCPICQQNTENIMNTAKELLTYLKRKKQQQKQEAEKQEEEKDSDDDEKPHECDDHHHHDHEDEPEEPENDSNVPEAEEKSDEEQEIMMEDVEGLEGGENDSESDDDDAEKD",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design must be focused on enhancing the efficiency of DNA repair. The designed protein sequence is ",
    "response#1": "FQNNYFFAYADITRGDFQLTMGLNEQELQSEIERLRPAEILMPETANVPAFITALKPHRQPDWYFDSDSSYRLICEYYQTTTIDGFGIAPDDPALSAAGCLLQYLHDTHKYHLPPS",
    "response#2": "FQNNYFFAYADITRGDFQLTMGLNEQELQSEIERLRPAEILMPETANVPAFITALKPHRQPDWYFDSDSSYRLICEYYQTTTIDGFGIAPDDPALSAAGCLLQYLHDTHKYHLPPA",
    "response#3": "FKTKYSKVILKNSEKIPQDLHEEVQEAFFTLRKHVCFFQDLVRIKGRGFVTPVSRILIGNPGCYDIHYSNPEISLACIALSRLNDYLQRESLLGLQEQDLAETTGPQNSPFGDGDQDYITETGAILQDQNPSLKSDSTILKERTSYNLTLLNYMDPQKMPSLKLEPYFGMGRMAVSWHKSNGKVNSGVDGGILEEKSAAGRDPDVWHVGLKVAWDIETPGLAIPLHQGDCYFMN"
  },
  {
    "reference": "MNLKTLLKNITIKTSLYVEDKDITSVCFDSRKVEKGSMFVAITGTQSNGHEYIDQAIRSGTTAIVCEQLPQQPLPDVSYIVVPNSHSALALIAANFYGNPSEKLTLVGVTGTNGKTTIATLLYQLFRKAGQKAGLLSTVAVYVDDQRFEATHTTPDPLQLNHYLHLMVQQGVKYCFMEVSSHGIDQHRTEGLQFAGGIFTNLTRDHLDYHKTFAAYRDVKKRFFDALPSTAFALVNNDDKNGAFMLQNTKAQKKTYALKTHADYKAYIIESRFDGLLLRINQHEVWTKLIGEFNAYNLLAVYATATLLGIDEIEALTLISAIEPVAGRFQYAVSATGVITIVDYAHTPDALENVLKTINDIRTHNETLTTVVGCGGNRDKGKRPIMADIATTLSDNVILTSDNPRNEEPEAILSEMQQGVQPQYFHKVQTITDRRQAIQSVCKNAQKGDIILIAGKGHETYQEIKGVRHHFDDFEIVREYLELYKK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The ideal protein would be one that plays a critical role in cell division, cell wall organization, regulation of cell shape, peptidoglycan biosynthetic process through ATP binding, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--D-lysine ligase activity, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, magnesium ion binding within cytoplasm.2. The protein must bind to Mg(2+) in order to perform its enzymatic function.3. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.4. The protein contains novel Mur ligase N-terminal catalytic, Mur ligase C-terminal domains that confer a unique function or activity.5. The protein's functional design necessitates the inclusion of a Meso-diaminopimelate recognition motif motif.6. The designed protein should have a binding site that is specific to the target meso-2,6-diaminoheptanedioate, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP ligand. The designed protein sequence is ",
    "response#1": "MLLKELLNDVKIENVTGSIESKEVDEKSIFFAIKGVSKDGHDFIEEAIEKSAPYVIAERNITNEVPQVEVVDSEHYFSLLSANFFGNPSKKLKLVGVTGTNGKTSIVSLLHELFINEGYKSGLISTIKVEYGDKIYDNTHTTPNALLINTYLSDMVGLGITHCFMEVSSHGITQHRIEGLVFKGGVFSNLSHDHLXYHGSFKKYRDTKKEFFDNLPKSAFALTNLDDKNGAFMLQNSKAKKYTYSVKHHADYKAKLLECQFSGMLLKIKSHEVWTSLVGNFNIQNLLAVFAVTGLKALGIQEKIIAKAILNVKGAEGRFETFQTTNGTTIIIDFAHSPDALENILETINSVRTRNETLITLLGCGGNRDAEKRPLMGLKATKFSDKVIFTSDNPRDEDPEEIITQMINGVPQEHFKKILKVTSREDAIAMAAELAEDGDIVLIAGKGHETYQEIKGVRHPFDDLAVAKECL",
    "response#2": "MKLADVLAGVPLVGADPEEITGVTTDSRAVRRGQLFAALPGTRVHGAAFIPMALEKGAGAILTDADGAALAADALAASDAALIVAEDPRAALAQTAAVVYGNPSERLRIVGVTGTNGKTTVSCLVRELMEYKGRRTGLIGTIEHILGENRIIDSFTTPDAVLLQKYFAEMVKQNLSTAVTEVSSIGLSLGRVRETKFLAGVLTNISLDHLDFHGSFEEYVAAKQQLFAALPEQGIAVVNLDCQYAQSFLASSPARGVSYAVHQEADYRAINLRFSSSGSSCDIWYQGEVFPCETSLVGEHNVYNILAALAVAAELGLAPEVIAAGLAAVPQVPGRFEPVLGSQPFSVVVDYAHSPDGLEHLLTSARELVEPGRRVIVVFGAGGDRDPRKRPRMGEVTARLADVVVVTSDNPRGEDPASIIDAIVGGMAAEAAAIVEPDRRAAVERALALAEPGDLVVLAGKGHEDYQIIGTERRHFDDREEAAAALAARAGGDA",
    "response#3": "MNVTGTSGKTLVVSFIRQIWHHLGFPAASLGTLGLIVEGRPLPPPTGTEGINTPNPVRLHQILQNLKEQQINHLAFEASSHGLHQHRLDSVRLKAAIFTNFSSEHLDYHHTLDAYFEAKVRLFQEIMIHESFAILNTDIAEYEQLLALCQKRRLKTITFGKAGEMIRLISDIPKDGCQDVFLSIEGKSYNFTLPFVGQFQVYNVMAALGIALALGVPAEKTVEALRSFGGVKGRMELFQVANKPKIVVDYAHTPDALEKALQALRVHCEGKLWVIVGCGGDRDKGKRPMMASIAEQLADYVVLSDDNPRGEMPSAIVDDMLAGMNNPAAAMIEHSRFEAAKIALAQAGTSDIILLAGKGHETYQEIAGVRHHFDDREEARAALKSRLEK"
  },
  {
    "reference": "MISLEDIRRDYLLGGLDLDQLDASPIAQFETWMAQAIESQIPDPTAMVLATANKDGFPSQRIVLLKKLDEQGFVFFTNYGSRKAQEIAEQSHVSLHFPWHMMERQVSVGGVASKISYAETLKYFVSRPKDSQIAAWASAQSKPISSRQLLLQQFNAIKTKFANGEVPLPDFWGGYRVVPQRMEFWQGGGARLHDRFVYEREGNSWAINRLAP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).2. The designed protein should be able to compete with endogenous proteins or inhibitors for FMN binding.3. The designed protein should have FMN binding to facilitate pyridoxine biosynthetic process.4. The protein should be optimized for activity in pyridoxal 5'-phosphate salvage, Cofactor metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MQQAIEENVQEPYAMSLATCGADNKPSVRIVLLREITDKGIVFYTNYESAKGQDIAENPNVEALFFWHKLERQIRISGSIAKIDADKSAAYFQKRPHDSQVGAWVSQPQSGEVANRDVMEQTFEQR",
    "response#2": "MQQAIEENVQEPYAMSLATCGADNKPSVRIVLLREITDKGIVFYTNYESAKGQDIAENPNVEALFFWHKLERQIRISGSIAKIDADKSAAYFQKRPHDSQVGAWVSQPQSGEVANRDVMEQTFEQIMWGGYRLKPDNWQFWQGRKSRLHDRLRYTLQGDAGWLRERLAP",
    "response#3": "MQQAIEENVQEPYAMSLATCGADNKPSVRIVLLREITDKGIVFYTNYESAKGQDIAENPNVEALFFWHKLERQIRISGSIAKIDADKSAAYFQKRPHDSQVGAWVSQPQSGEVANRDVMEQTFEQL"
  },
  {
    "reference": "MESECLTPEWASQPCLMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLHFADSKTLKEEKREELYESLKLDKSLGWAVDVIDPRELSAKMLAKNKTNLNEISHNSAMGLIKRVLDMGVLLTEAYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTRDRALKEWLVEETGEDINRNFGSGYPGDPETKAWLVQHKHSVFGFPSLVRFSWGTCTTHLKGEVEVAWEADENEESGNGSSSKRQAKLSSFGFKTCEKRSEEIESSGKGRCKFLQARKIQQLTQF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be optimized for metal ion binding. The designed protein sequence is ",
    "response#1": "LRTSRTNPAKRPDGLDTCIATLKKNGADALIAIGGDDTLSVATKLFDKGVRVVGVPKTIDNDLAGTDFTFGFDTAVNVVTEAIDRVHTTAEAHNRVMVVEVMGRDAGWIAIYSGIAGGADVILIPEQPFDVDKVAAKIRRRHERGRYFSIVVVAEGAKFASKNGPVMQSGETDEFGHARLGGIANTLALELEKRTGFETRTVVLGHIQRGGSPTAFDRVLATRYGLGAIDALVS",
    "response#2": "LRTSRTNPAKRPDGLDTCIATLKKNGADALIAIGGDDTLSVATKLFDKGVRVVGVPKTIDNDLAGTDFTFGFDTAVNVVTEAIDRVHTTAEAHNRVMVVEVMGRDAGWIAIYSGIAGGADVILIPEQPFDVDKVAAKIRRRHERGRYFSIVVVAEGAKFASKNGPVMQSGETDEFGHARLGGIANTLALELEKRTGFETRTVVLGHIQRGGSPTAFDRVLATRYGLGAIDALAG",
    "response#3": "LRTSRTNPAKRPDGLDTCIATLKKNGADALIAIGGDDTLSVATKLFDKGVRVVGVPKTIDNDLAGTDFTFGFDTAVNVVTEAIDRVHTTAEAHNRVMVVEVMGRDAGWIAIYSGIAGGADVILIPEQPFDVDKVAAKIRRRHERGRYFSIVVVAEGAKFASKNGPVMQSGETDEFGHARLGGIANTLALELEKRTGFETRTVVLGHIQRGGSPTAFDRVLATRYGLGAIDAVAALR"
  },
  {
    "reference": "MNPGQLAEDRAMKHLCAHGLRLEARNVRYPFGEIDLVMREGRVYVFVEVKFRTPKGFGDAVQALSAAQQQRLRRAATHYLQCHRIDAPCRFDMVAITGDKLEWIKDAF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must have high specificity towards nucleic acid binding. The designed protein sequence is ",
    "response#1": "GFSSLM",
    "response#2": "MK",
    "response#3": "MP"
  },
  {
    "reference": "MLTHKKIVVGITGGIAAYKTIELIRLLRTANAEVRVVLTPAAEAFVTPLTLQAISGNAVSTSLLDPQAELAMGHIELAKWADLVVIAPASADFIARLRVGMGNDLLSTLCLATAAPILLAPAMNQQMYKRSVVQKNLQKLRKQGVLTVGPNEGFQACGDVGTGRMSEPSEIFHAIRDHFAEREDLADLTITITAGPTQEAIDPVRYISNHSSGKMGFAIADAFAKRGAKVMLIAGPVNLPTPQNVDRVDVISAQEMAQQAVSSAQKSAIFIGCAAVADYRVEQVAEQKLKKTDDSDTLTLTLVKNPDIIANVAHLTENRPFTVGFAAETQNVAEYAKSKLQRKNLDLICANDVSGGQVFGQDHNALQLFWQNGEKTLPLANKTELAKTLVNEIIEQYRGK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must have a Proton donor that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "SEWFFLDFINPSSANLIKARRLIFSARSPYQEIEILDTQAFGKCLMLDGRMQSAQADEFIYHEALVHPAMVTHPNPESVLIVGGGEGATLREVLRHRTVRRAVMVDIDRQVVEACRLFLPEWSDGAFEDPRTELVIDDARHYLTSTDEQFDVIIMDITEPLENGPSAPLVTYEMFQIVRSHLKPQGIVAHQSGSTGTSELHFFASMYKTLRAVFRYVAPYSIQITSFAMPWGIVIASR",
    "response#2": "SEWFFLDFINPSSANLIKARRLIFSARSPYQEIEILDTQAFGKCLMLDGRMQSAQADEFIYHEALVHPAMVTHPNPESVLIVGGGEGATLREVLRHRTVRRAVMVDIDRQVVEACRLFLPEWSDGAFEDPRTELVIDDARHYLTSTDEQFDVIIMDITEPLENGPSAPLVTYEMFQIVRSHLKPQGIVAHQSGSTGTSELHFFASMYKTLRAVFRYVAPYSIQITSFAMPWGIVIASV",
    "response#3": "SEWFFLDFINPSSANLIKARRLIFSARSPYQEIEILDTQAFGKCLMLDGRMQSAQADEFIYHEALVHPAMVTHPNPESVLIVGGGEGATLREVLRHRTVRRAVMVDIDRQVVEACRLFLPEWSDGAFEDPRTELVIDDARHYLTSTDEQFDVIIMDITEPLENGPSAPLVTYEMFQIVRSHLKPQGIVAHQSGSTGTSELHFFASMYKTLRAVFRYVAPYSIQITSFAMPWGIVIASV"
  },
  {
    "reference": "MSSKMVISEPGLNWDISPKNGLKTFFSRENYKDHSMAPSLKELRVLSNRRIGENLNASASSVENEPAVSSATQAKEKVKTTIGMVLLPKPRVPYPRFSRFSQREQRSYVDLLVKYAKIPANSKAVGINKNDYLQYLDMKKHVNEEVTEFLKFLQNSAKKCAQDYNMLSDDARLFTEKILRACIEQVKKYSEFYTLHEVTSLMGFFPFRVEMGLKLEKTLLALGSVKYVKTVFPSMPIKLQLSKDDIATIETSEQTAEAMHYDISKDPNAEKLVSRYHPQIALTSQSLFTLLNNHGPTYKEQWEIPVCIQVIPVAGSKPVKVIYINSPLPQKKMTMRERNQIFHEVPLKFMMSKNTSVPVSAVFMDKPEEFISEMDMSCEVNECRKIESLENLYLDFDDDVTELETFGVTTTKVSKSPSPASTSTVPNMTDAPTAPKAGTTTVAPSAPDISANSRSLSQILMEQLQKEKQLVTGMDGGPEECKNKDDQGFESCEKVSNSDKPLIQDSDLKTSDALQLENSQEIETSNKNDMTIDILHADGERPNVLENLDNSKEKTVGSEAAKTEDTVLCSSDTDEECLIIDTECKNNSDGKTAVVGSNLSSRPASPNSSSGQASVGNQTNTACSPEESCVLKKPIKRVYKKFDPVGEILKMQDELLKPISRKVPELPLMNLENSKQPSVSEQLSGPSDSSSWPKSGWPSAFQKPKGRLPYELQDYVEDTSEYLAPQEGNFVYKLFSLQDLLLLVRCSVQRIETRPRSKKRKKIRRQFP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III..2. The protein should be optimized for stability and solubility when expressed in nucleoplasm, cytosol where it will be involved in snRNA transcription by RNA polymerase II, snRNA transcription by RNA polymerase III. The designed protein sequence is ",
    "response#1": "EARSYGLSCGRVSDGSRVSVFHVKLTDSALKAFESYRAHQDSVSLRPSIRFEGSQGHISIPQPDCPEEVRAFSFYLSNIGRDSPQGSFDCIQQYVSSYGDVHLDCLGSIQDKVTVCATDDSYQKARQSMAQAEEETRSRSAIVIKAGGRYMGKKVQFRKPAPGAADAVPSRKRATPINLASAIRKSSGSGASSVVQRPFRDRVLHLLALRPYRKAELLLRLQKDGLTQADKDTLDSLLQQVASVNPKDGTCTLQDCMYKSLQKDWPGYSEGDRQLLKRMLMRKR",
    "response#2": "SEYLAPQEGNFVYKLFSLQDLLLLVRCSVQRIETRPRSKKRKKIRRQFPVYVLPKVEYQACYGVEALTESELCRLWTESLLHSNSSFYVGHIDAFTSKLFLLEEITSEELKEKLSALKISNLFNILQHILKKLSSLQEGSYLLSHAAEDSSLLIYKASDGKVTRTAYNLYKTHCGLPGVPSSLSVPWVPLDPSLLLPYHIHHGRIPCTFPC",
    "response#3": "SEYLAPQEGNFVYKLFSLQDLLLLVRCSVQRIETRPRSKKRKKIRRQFPVYVLPKVEYQACYGVEALTESELCRLWTESLLHSNSSFYVGHIDAFTSKLFLLEEITSEELKEKLSALKISNLFNILQHILKKLSSLQEGSYLLSHAAEDSSLLIYKASDGKVTRTAYNLYKTHCGLPGVPSSLSVPWVPLDPSLLLPYHIHHGRIPCTFPC"
  },
  {
    "reference": "MHRLTNRHILLGITGGIAAYKSAELTRLLKKAGADVRIVMTPAATEFITPLTLQALSGHPVHLHLLDPEAEAGMGHIELAKWADLILIAPASADFIARCAAGMGNDLLTTLCLATTAPVCLAPAMNQAMWRDPHTQSNIGSLTATGMKVFGPDSGEQACGDTGPGRMLEPQALAELIAAEFEYLALSGKTVFITAGPTREPLDPVRFISNHSSGKMGYALAQAAIDAGARVKLISGPVNLTAPERVECIQVETAEEMLTASLEGINSCDLFIGAAAVADYRPVAVAEHKIKKGNEEIMELRLVKNPDIIASVAALSQRPFTVGFAAETHDLLSYARSKLERKKLDLIIANDVSVPGIGFNSDDNRVTLVSASGEVPLPQTSKRLLARQLIEHIAQHFNQQMAANEKTTG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The Proton donor of the protein must be engineered to enable effective catalysis of H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.2. The protein must exhibit phosphopantothenoylcysteine decarboxylase activity, phosphopantothenate--cysteine ligase activity.3. The designed protein should have a binding site that is specific to the target CTP ligand.4. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..5. The designed protein have DNA/pantothenate metabolism flavoprotein C-terminal domains with specific sequence motifs that are crucial for its activity.6. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis, coenzyme A biosynthesis pathway to avoid interference with native metabolic processes.7. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "MQIVLGVAGGIAAYKAVLLLRLLREAGHDVRVVPTRAALEFVGRPTWEALSGQSATPEVFDDVAHVPHVALGQHADLVIVAPATADLLARAAAGRADDLLTSTLLTARCPVLMAPAMHTEMWDHPATRANVATLRARGVHVLDPASGRLTGADTGPGRLPEPDQIAAAAAALLGEPAAAGPADLAGRHVVISAGGTREPLDPVRYLGNRSSGRQGYALARTARDRGARVTVVAANVSLDDPAGVDVVEVETTDQLRTAVRAAARTADVVVMAAAVADFRPVAAAGHKVKKRSDGVVDPILLEANPDILAELTAERLRPGQVIVGFAAETGDEHTTVLEYGRAKARRKGADLLAVNAVGHGLAFGTTDNAVTILGADGTETPVPYGPKEVLADAVWDLVADRL",
    "response#2": "MQIVLGVAGGIAAYKAVLLLRLLREAGHDVRVVPTRAALEFVGRPTWEALSGQSATPEVFDDVAHVPHVALGQHADLVIVAPATADLLARAAAGRADDLLTSTLLTARCPVLMAPAMHTEMWDHPATRANVATLRARGVHVLDPASGRLTGADTGPGRLPEPDQIAAAAEAVLAGPSPALRGQRVLVTAGPTYEAIDPVRGITNRSSGKMGFAIARAAREAGADVTLVAGPVHLPTPRGVRRMDVRSAQEMQRAVESEVLHASVFVATAAVADWRPASVAEQKIKKDGSGKPPALDFVENDDILAGVAQSSRAKSGALYCVGFAAESHELLAHAQAKRVRKGVPLLVGNIGPATFGQDDNALLLFDDAGRHPLPRGSKSALAHALVDEISARLAP",
    "response#3": "MQIVLGVAGGIAAYKAVLLLRLLREAGHDVRVVPTRAALEFVGRPTWEALSGQSATPEVFDDVAHVPHVALGQHADLVIVAPATADLLARAAAGRADDLLTSTLLTARCPVLMAPAMHTEMWDHPATRANVATLRARGVHVLDPASGRLTGADTGPGRLPEPDQIAAAAHLLLRGEAAVALAGRHVVISAGGTREPLDPVRYLGNRSSGRQGYALARTARDRGARVTVVAANVSLDDPAGVDVVEVETTDQLRTAVRAAARTADVVVMAAAVADFRPVAAAGHKVKKRSDGVVDPILLEANPDILAELTAERLRPGQVIVGFAAETGDEHTTVLEYGRAKARRKGADLLAVNAVGHGLAFGTTDNAVTIVDAAGQEVARADGSKEDVAAGVWDVVVRRLGA"
  },
  {
    "reference": "MEFVAATNNAHKLAEMRRILVRMGHTVLSQREAGVLLEPEETGATFEANAAIKAQAVCEACRKPTIADDSGLCVDALGGAPGVYSARYSGVHGDDGANNRKLLAALEGVPAARRAAHFTSAVCVWLPGGRHAVYVGRCPGAIGFEEKGENGFGYDPLFIPDEVGMPGRGAACKANTAQRTYAQLSADEKDAISHRARALALMEQELPAFLADIGVLGAVCGPACPSKE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in dITP + H2O = dIMP + diphosphate + H(+) are properly positioned for optimal catalysis.2. The protein design must be able to perform metal ion binding, ribonucleoside triphosphate phosphatase activity, nucleotide binding, dITP diphosphatase activity, XTP diphosphatase activity in order to contribute to purine nucleoside triphosphate catabolic process, nucleotide metabolic process.3. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.4. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.5. The substrate, Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MYKIVLASNNAKKLVELQQILAEFELEIIPQGELGVAEAEEPHCTFVENALAKARHASQHTGLPAIADDSGLVVDALAGAPGIYSARYAGEHGNDAANNAKLLHELAGVPDERRGAQFFCALALVRHADDPLPIIAEGEWHGRILHAPRGAGGFGYDPLFWVPELGCTAAELEPAVKNRLSHRARALTQMRAMLQRLPS",
    "response#2": "MKILIASNNAGKLREFRALLAPLGIELVAQTALGVTGPVEDGLTFVENALAKARHASAATGLPALADDSGLSVAALDGAPGIYSARYAGEPKSDARNNAKLVADLRGQADRGAHYVCVLALVRHADDPSPIIAEGVWEGRILTEHRGANGFGYDPLFYVPQYQCTSAQLTAEQKNALSHRGRALRALLAKLPAT",
    "response#3": "MKILILAGGTSRRLGLDSPKALVKLKGKSLIEHAINSLENESLIDNIFVTTNKKYAEILKSEFKCILVDGGETRQDTVKNGLEVCKDVDVXAIHGDCPFAKXDMIKSLIDNSXKADCVVPTIKVKDATYFSEDNIDRKNIKRVQTPQAFKFSVLKEAHXKXKNXSXEXTDESSIMXNXGFVKLIXGDXNXKXTTXKDLXKIXGXFXSXNXFXKNKNKKXKXI"
  },
  {
    "reference": "MEHMMAIVMAAGHGKRMKSNRPKVLHTVCGKPMIDRVVAAAQDATGNKPVVVVGVGREAIMEHLGDRADFAVQAEQKGTGHAVMMAEPYLSGKSGYAVVLAGDMPLLMADTLRQLYAMCKEERLDAVVLTSEPDDPTGYGRVIRDTTGSILRIVEEKDANQEEKAIREVNSSVYCFRIESLLRCLGQLSCDNAQGEYYLTDCIAMLAATGRVGGLLVDAQQCSGVNDKAQLAAAEAIMRRRINHGYLVGGVIMMDPDHTYIEDGAVIGQDTILYPGCHIRGKSAIGADCVIESGTVIDGSTVGDRCHIRDSVIMDSTVGNDVTIGPFAYLRPATVVEDGVHIGDFVEIKKARIGEGTKLPHLTYVGDADVGAHCNIACGTIFANYDGLKKYQTTVGDGVFIGCNVNLVAPCTVGDGAYVAAGTTVVGEVPAGALSVGRSQQYIKEDWADEKRKKGALREVY",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should contain one or more MobA-like NTP transferase domains that are essential for its function.2. The protein should be optimized for activity in UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).4. The protein should have a high affinity for Mg(2+). The designed protein sequence is ",
    "response#1": "VIMAAGKGTRMKSARPKVLHKLAGRPMLSHVIATTSTLAAAQQVVITGHGAEMVESSMKAAFATAPLQFVRQEPQLGTGHAVQQAVPVLPDDGITLILNGDTPLIEASTAQALIDACGGKQLALLTVELADPTGYGRIVRDQHGRVLRIVEQKDASAEELAIREVNTGILAAPTLLLKDWLARLDNQNAQGEYYLTDIVAMAVADGVEIVTTQPADESEVLGVNSPSQLAALERAHQLRQAGALMEQGVTLRDPFRVDVRGEVSVGRDIMIDINVILEGKVVIEDDVQIGPNCVIKDSILRRGAVVKANSHLDGAVLGEGSDVGPFARLRPGSVLEAKAHVGNFVELKNARLGEGAKAGHLSYLGDAEIGARSNIGAGTITCNYDGANKFRTVMGEDVFIGSNSALVAPVQLGDGATTAAGSIITQNVEAAQLAVGRARGVSILDPATTWIDVTVAVDRDAVIDQNTQLRGGSVVGAEAVVGPDVTLIDTVVGAGASVLRSHAVQAEVGPGASVGPYAYLRPAARLAERSKVGTFVEVKNSELGVGAKVPHLSYVGDATIGAKANIGAATIFVNYDGVNKHRTTVGEAAFVGCDTSLIAPVEVGAGAYVAAGSAISQDVPPGALGVTRAPKAAKREDWVRPVKTKK",
    "response#2": "MTTATPKVLHFISGKAIIDHVLSQITKLNPGELRVVVGAGREAVESHVKQIAPNAVTVFQAERNGTGHAVQLALADLKATGTVLICAGDTPLLRSETLGEFIAAHVEAVNSVSVLTAEFPDPTGYGRILRNEAGEIVAIVEERDASDEIKLIDEINTGVYLFDIAALRTSVAKLKADNSQGELYLTDVISDIKASGLRAAAVLSNDFDETLGVNDRVALSEAERLLNARIVRKHMINGVTIQDPANTYISADAEIGRDTVLMPGTVIEGRTVIGEDCLIGPGSQIRDSVIGDRTAIRQSVVMSSKIGEDTAVGPFAHIRPETDLGNSVKIGNFVEVKKSNLGEGSKVSHLSYIGDSDIGERVNIGCGTITVNYDGVNKFRTTVEDDAFVGCNSNLVAPVTVGKGAFVAAGSTVTKNVPENALAIARSRQENKPGYAEKLRARASAKKPKDDS",
    "response#3": "VVLAAGQGSRMNSDLPKVLHKVGGAPLFAHALNAGAALEPERSVLIAGHGAEAVRAEASEWDEDLVVALQEQQLGTAHAVAQARDALEGAEGDVFILYGDTPFIRPETLSMMAAARAGGAAVVVLGFEAADPGRYGRLVTDGDRLLRIVEAKDASAEELAITLCNSGVMCADAAVLFELIEAVGNDNASGEYYLTDIVEIARARGLRAVAVTCDEAETLGVNSRADLAEAEAAFQARMRAQMMADGVTLIAPETVFFAHDTKIERDVVIEPNVVFGPGVVIEEDVVIHAHSHVEGAHLKRGAHAGPFARLRPGAELGEGSKVGNFVEIKKSQLAEGAKVSHLTYIGDASIGANANIGAGTITCNYDGYDKHRTVIGENAFIGSNTCLVAPVTVGAGAFTATGTIVTEDVPADALALARTPQLSKEGWALRFREQMAAAKKAKAKS"
  },
  {
    "reference": "MVTAMNASDGTEQMLEPYNFELYWNMRPLLEKYWATSFPIALVYLLLIFVGQNYMKARKGFNLQGPLILWSLGLAIFSILGALRTWGYMATLVLRGSLKKTVCFSTSANTPIIGFWSCLFVLSKVIELGDTAFIILRKRPLIFVHWYHHSSVLVYTSFGYKNKVSAGGWFMTMNYGVHAIMYIYYTLRAAKVKSPRWLPMLITSLQILQMFMGAIVGILTYLWRQEQGCYTTMEHCFWSLVLYTTYFILFAHFFHQTYLVPKVKAKIKSQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should interact with enzymes and substrates within the polyunsaturated fatty acid biosynthesis, Lipid metabolism pathway to enhance or regulate its activity.2. The protein have enhanced a very-long-chain acyl-CoA + H(+) + malonyl-CoA = a very-long-   chain 3-oxoacyl-CoA + CO2 + CoA.3. The protein design must feature the Di-lysine motif motif.4. The protein should have a Helical domain that is long enough to span the entire membrane.5. A protein with very-long-chain 3-ketoacyl-CoA synthase activity functions is required to enable fatty acid elongation, monounsaturated fatty acid, long-chain fatty-acyl-CoA biosynthetic process, very long-chain fatty acid biosynthetic process, fatty acid elongation, polyunsaturated fatty acid. The designed protein sequence is ",
    "response#1": "MSESGKVTITIDGQEVTVPKGTTVLDACRELGVDIPHFCWHPKLDPVGMCRMCLVEVEGQPKLVPSCALPVMEGMKVKTDSEKAKAARKALLEFILLDHPLDCPICDQGGECKLQDYYMTYGHGTSRLEYEEKVHPKNPLYLGPQIVLDAERCVVCTRCVRFTDDVAGDPQIGVLDRGNHTEISTFDERPFDSYFSGNTIQICPVGALTSTAYRFRPFDLRRTESVCELCSVGCATKVETRNDEVVRVEARDDPAINDEWICDKGRFGYEYVNHADRLTTPLIRKDGELQPASWEEAIDLVADGFAEIKGDHGPDAVGVLSSAKATNEDLYLFQKLVRAVIGTNNVDHRLRQSDLEGRASVGATLGPGLGVPITDLAHTESILMIGSNITAEHAVLSLRIRKAVEDNGGKLIVVDPRRTQLAEWATIHLHPRPGSDVALLNAMIKVILTEDLQDKEYIDERTEGIESFAGAVAEFTPEQVEEVTGIAAESIEDVARMYAEHGRTSTIIGSGVNLARHQDDVRGIVNLSLLTGNIGRPGTGVNPLRGQNNVQGASDAGLIPNHYPAYQAVSDPAVADKFAKAWGVPAEGLTERPGLNMIEVIDEVHQGDIKGMYVIGENPAMSDPDAGHTERALSKVEFLIVQDIFMTETAEHADVILPAASFAEKDGTFTNLERRLQNLNPVVEAPGQARPDWQIL",
    "response#2": "MAETLTSLKSQLLSLRKERDALLAQLQQLESQLKDESARGKNTPSTTSSPSGTVTVGGRDGKGWRAKYPPMRDKLYRKIAEKHPDLSIEQLRAEFDLPEQTTTTSTATGTKRKADEDAAAKEPRKKVKKEEHEEAPVSETPTVAETTKEEVVAPSVKEEPVSDVPVAKEEPVEEKKVAEVPATEAVEEKPAVEEKPAVEEKPAVEEKVAVEEKPAVEEKPAVEEKVAVEEKPAVEEKPAVEEKPAVEEVVVKEEPVAKVEAKETKVEEEKPAVEETKVEEEKKVEETKVEEAKVEEEKKVEETKVEEAKVEEEKKVEETKVEETKVEETKVEETKVEETKVEETKVEETKVEETKVEATKVEETKVEATKAEEVKVEETKVEATKAEETKVEETKVEETKIEETKVEETKVEETKVEATKVEETKVEETKVEETKVEATKAEETKVEETKVEETKVAETKVEETKVEETKVEETKVEETKVEETKVEETKVEATKAEETKVAETKVEATKAEETKVAETKVEATKAEETKVAETKVEATKAEETKVAETKVEATKAEETKVAETKVEETKAEETKVEETKVEATKAEETKVAETKVEETKVEETKVEETKVEETKVEETKVEATKAEETKVAETKVEETKVEETKVEETKVEETKVEATKVEETKVEETKVEETKVEETKVEATKAEETKVAETKVEETKVEETKVEETK",
    "response#3": "MVSRAFYGTALAKSVCLATLFYTMAYTYYEVFFHPLRSFPGPFLAKVTPFWPLWHHLRGSLVWCLRDLHRRFGPIVRYGPNTLSFATPAAYKDIYANPTTRKSPFYAYIGQDGRQSILSTVHRDEHARRKRLLSHAFSDKALRDQEPLIQGYVDLLVARLKEQAGSGQAGRVDMVRWYNFTTFDIVGDLAFGESFGCLEDSRYHPWVELMFSGIKALTYALTLSRYPRIGQIVLFLLIPGSLRKRQVEHHAFTMEKVERRLQRGTTRPDFMSHILRHNDTKEGLTDEELVANSSVLITAGSETTAALLSGITYLLLKNPSVYAKLTDEVRSAFSSEEDITLLSVNGLSYLNAVIEEGLRIYPPVPSGVPRVSPEGGDTICGTFVPGGTIVGVHQWAAYRSPQNFHKAHEFLPERWLGDKDFAKDDLQAVQPFLLGPRNCIGRNLAYHEMRLTLAKVLWNFDIELDEKSKDWHRQKSVYAFWEKMPLMVKLTPVR"
  },
  {
    "reference": "MSVTTPQGFVAAGAACGIKPSGDLDLALVATEDGRPVTAAAVFTSNLAAAPPVQLSRSNLAATGGRASAVIVNSGNANAATGAPGRAHAERIAELVADRLGCAAREVLVCSTGLIGIPLPIGAIESGIGALVDRVARGATEDDAAAHAILTTDTRAKGVLLEEDGFTLGGMAKGAAMLAPNMATMLAVLTTDAALDHPAIDEALRAAVTSSFNAMTVDGCTSTNDTAILLASGRRGPVDESSFAAALARACGSLSQQMAADAEGATKMARIRVVGAASDGDAATAARKVAQSQLVQCSLHGEDPYWGRVVSELGSSGAAFDPDRVSVAYGGVVVCRDGVACDHDRAVVAAHMSERDISISVNLGLGSGQAAIVFTDLGPGYIAENMTTS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate binding.2. For general function, the protein need meet that Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.3. The protein design should take into account the physiological conditions of the L-arginine biosynthesis, N(2)-acetyl- L-ornithine from L-glutamate: step 1/4, Amino-acid biosynthesis pathway.4. We are looking for a protein with broad substrate specificity for acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate.5. A protein with glutamate N-acetyltransferase activity, acetyl-CoA:L-glutamate N-acetyltransferase activity, methione N-acyltransferase activity is needed to enhance arginine biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "MQSPTQALLAPWLASLPHDDPDLAXVXXVXAXRXGXGXGXRXGXGXGXRXRXSATXSXLXVXDPXAXAPXLHVADARRAFPLIXAHXYXHPATQLXVXGVXGX",
    "response#2": "MSKKYDLSAIGNGIAXLSEQGLLSAXRXAXGGXLAXLSSXDXAXPXADXALXGXSASXATXGXTXGXSXIAXDXDXXAXLDAXXVXKXNXAXKLXXXIAXAGXAXSXAXGXTXAXAXAXTXPXLRXGSXXKVANAXYXXXA",
    "response#3": "MTQEFAHSFSWDDPTLGEWLALTEERRWPAGGATVAVGLSGDVVHQAGGVVQALQPQA"
  },
  {
    "reference": "MRVKGIRKNYQHLWRWGIMLLGILMICRAADNLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNLNVTNLKNVTNTNSSIGGEKMEGGEMKNCSFNVSTSIRNKIKNEYALFYKLDIVPIDKDNTSYTLINCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNDKKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVIRSENFTNNAKTILVQLNASIEINCTRPHNNTRRSITIGPGRAFYATGQIIGDIRQAHCNLSSVKWNNTLKQIVEKLREKFGNKTIVFNQSSGGDPEIVMHNFNCGGEFFYCNSTQLFNSTWHVNSTWNDTEGANNTITLPCRIKQIINRWQEVGKAMYAPPIEGQIRCSSNITGLLLTRDGGRDKNQTEIFRPGGGDMRDNWRSELYKYKVVKSEPLGVAPTKAKRRVVQREKRAVGTLGAMFLGFLGAAGSTMGAASMTLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSQDYIWNNMTWMEWEREINNYTGLIYNLIEESQNQQEKNEQELLELDKWAGLWNWFDITNWLWYIKIFIMIVGGLVGLRIVFTVLSIVNRVRQGYSPLSFQTHLPAPRGPDRPEGIEEEGGERDRDRSGRLVDGFLTLIWVDLRSLCLFIY",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Transmembrane protein gp41: Acts as a class I viral fusion protein; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain2. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.3. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.4. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.5. The protein must contain a signal peptide for proper functionality.6. The designed protein should have structural molecule activity within virion membrane, host cell endosome membrane, viral envelope, host cell plasma membrane, plasma membrane to regulate positive regulation of receptor clustering, fusion of virus membrane with host plasma membrane, positive regulation of establishment of T cell polarity, viral protein processing. The designed protein sequence is ",
    "response#1": "MKVKGTRKNYQRLWRWGTMLLGMLMICSATEQLWVTVYYGVPVWKEASTTLFCASDAKAYEREAHNIWATHACVPADPNPQEIELKNVTEDFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNAISENNCTNTTSGNNNVTLNNTCSIPEMKNCSFNVTTVLRDKRKQEYALFYRLDIVEISNDTSYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCNDKKFNGTGPCHNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIMIRSENLTNNAKNIIVQFNESVEISCIRPNNNTIKSVHFGPGQALYATSRIIGNIREAHCTINRTAWNNTLQKVAKTLGERYSKMIIFAPSSGGDLEITTHSFNCGGEFFYCNTSGLFNGTWINNTWTWLNSTKSNETNTNVTIPCRIKQIINMWQGVGKAMYAPPIKGVIKCTSNITGIILTRDGGSNATNETFRPGGGNMKDNWRSELYKYKVVRIEPLGVAPTRAKRRVVEREKRAVGIGAMFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLRDQQLLGIWGCSGKLVCTTAVPWNSSWSNKSKTEIWDNMTWMQWDKEISNYTNTIYKLLEDSQIQQENNEKDLLALDKWNNLWSWFNITNWLWYIRIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLLFQTLIPNSRDPDRPG",
    "response#2": "MRVMGILRNCQQWWIWGILGFWMLMICNVVGNLWVTVYYGVPVWKEASTTLFCASDAKAYEREAHNIWATHACVPADPNPQEIELKNVTEDFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNAISENNCTNTTSGNNNVTLNNTCSIPEMKNCSFNVTTVLRDKRKQEYALFYRLDIVEISNDTSYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCNDKKFNGTGPCHNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIMIRSENLTNNAKNIIVQFNESVEISCIRPNNNTIKSVHFGPGQALYATSRIIGNIREAHCTINRTAWNNTLQKVAKTLGERYSKMIIFAPSSGGDLEITTHSFNCGGEFFYCNTSGLFNGTWINNTWTWLNSTKSNETNTNVTIPCRIKQIINMWQGVGKAMYAPPIKGVIKCTSNITGIILTRDGGSNATNETFRPGGGNMKDNWRSELYKYKVVRIEPLGVAPTRAKRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARLLAVERYLQDQQLLGIWGCSGKLICTTTVPWNNSWSNKSLDDIWNNMTWMQWDKEVSNYTQTIYTLIEQAQDQQEKNEHELLELDKWASLWSWFSITNWLWYIKIFIMIVGGLIGLKIVFAVLSIVNRVRQGYSPLSFQTLIPNPRGPDRLG",
    "response#3": "MRAKGIQRNYQHLWRWGTMLLGMLMICSATEKLWVTVYYGVPVWKEASTTLFCASDAKAYEREAHNIWATHACVPADPNPQEIELKNVTEDFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNAISENNCTNTTSGNNNVTLNNTCSIPEMKNCSFNVTTVLRDKRKQEYALFYRLDIVEISNDTSYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCNDKKFNGTGPCHNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIMIRSENLTNNAKNIIVQFNESVEISCIRPNNNTIKSVHFGPGQALYATSRIIGNIREAHCTINRTAWNNTLQKVAKTLGERYSKMIIFAPSSGGDLEITTHSFNCGGEFFYCNTSGLFNGTWINNTWTWLNSTKSNETNTNVTIPCRIKQIINMWQGVGKAMYAPPIKGVIKCTSNITGIILTRDGGSNATNETFRPGGGNMKDNWRSELYKYKVVRIEPLGVAPTRAKRRVVEREKRAVGLGAVFLGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLHEIWDNMTWMEWEREINNYTREIYTLIEKSQNQQEKNEQELLELDQWASLWNWFDITKWLWYIKIFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTRLPAQRGPDRPEGIEEE"
  },
  {
    "reference": "MLFAPPNLETKELFWIIYILILIPKVFAKVMSVRELFPFFKWGFNIRRSNFLVPILSNNVIVTGEEAVQYEKPLPYIPQHNQQQQQKQQPSQDYIQQPQNDNNINSGKEQEQQQQQQQQQQQTPDITNQPTKTNKKIPIKELSNEEILIKLEKGEVLAYRLENELGDCSRAVEIRRMLLEKQLSKKIEPIPHEGFDFAKVQGQCCENVIGYVPIPVGTAGPIQLNGQLVTIPMATTEGCLVASTHRGCKAITESGGAKCTITSRGMTRAPVVRFSDIVKASEFVSWINDTDNYQALKAVFDSTSRFARLSAIKCTIAGRSVYIRFKCDTGDAMGMNMVSKGVEAVLEHLKIIFDDMTLLSISGNMCTDKKPSSINWTEGRGRSVVCEAMITGDVVQRVLKTNVQALVDLNIAKNLIGSAMAGSIGGFNAHASNIVTAIFLATGQDCAQNVESSNCITQMEACNDGQDLYITVTMPSIEVGTVGGGTSLPAQSACLDIIGVKGSSSSKPGANADQLAKTIASAVMAGELSLMSALSAGHLMKSHLQYNRAKTN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have hydroxymethylglutaryl-CoA reductase (NADPH) activity and be active in peroxisomal membrane.2. The protein must exhibit the following characteristics: It is the rate-limiting enzyme of the sterol biosynthesis.; This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis.3. The designed protein must have a Proton donor that is structurally stable and resistant to denaturation.4. A protein that can modulate the overall flux of metabolites through the Metabolic intermediate biosynthesis, (R)-mevalonate biosynthesis pathway, enhancing or suppressing its activity as needed.5. The designed protein's functionality will benefit from Polar residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "LKPLAIIKGWGEAAHQPADFTWAPSLAVPKALKHAGIEDINSVDYFEFNEAFSVVGLVNTKILKLDPSKVNVYGGAVALGHPLGCSGARVVVTLLSILQQEGGKIGVAAICNGGGGASSIVIEV",
    "response#2": "VWFHCDGKEPEWEIPDISEISTGKFTYKGRVEHHANTHIQDVPENGSDLAHLSHLHVPHAMSGANLSTQYSSWWNFAEHIFKAQCIGPADGEPHISLFYLTHYLHVFKRFKLLSLNLNVYQIGPGIVHLHFDSPFGKGVFVQTLTPVEPLHLVLTHNLHASWSIPVWLGRIFLYLEAIQVDRDVMIWNNKTFEPRPKLLKEDSLIAKYRRWYSQFL",
    "response#3": "VWFHCDGKEPEWEIPDISEISTGKFTYKGRVEHHANTHIQDVPENGSDLAHLSHLHVPHAMSGANLSTQYSSWWNFAEHIFKAQCIGPADGEPHISLFYLTHYLHVFKRFKLLSLNLNVYQIGPGIVHLHFDSPFGKGVFVQTLTPVEPLHLVLTHNLHASWSIPVWLGRIFLYLEAIQVDRDVMIWNNKTFEPRPKLLKEDSLIAKYRRWYSQFL"
  },
  {
    "reference": "MAADLGQIPDVDIDSDGVFKYVLIRVHLAEPSGDPAKECKEIVRGYKWAEYHADIYDKVSGELQRNGYDCECLGGGRISHQSQDRKIHVYGYSMGYGRAQHSVSTEKIKAKYPDYEVTWADDGY",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have transmembrane transporter binding, protein histidine phosphatase activity within nuclear body, plasma membrane, nucleoplasm to regulate negative regulation of lyase activity, negative regulation of T cell receptor signaling pathway, protein dephosphorylation, negative regulation of ATP citrate synthase activity.2. The Proton acceptor of the protein must be engineered to enable effective catalysis of H2O + N(tele)-phospho-L-histidyl-[protein] = L-histidyl-   [protein] + phosphate.3. The protein should be able to bind substrate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "TVSHEIRTPMNGVLGMLKMLMDTDLDAKQMDYAQTAHGSGKDLTSLINEVLDQAKIESGRLELENVPFDMRFILDNVSSLLSGKANEKGIELAVYVSSQVPDVVVGDPSRFRQIITNLVGNSIKFTQERGHIFISVHLADEVKEPLTIEDAVLKQRLALGCSESGETVSGFPAVF",
    "response#2": "IPSAIDQRTFEEYTERTNFERPLTSGVAYALKVPHSEREKFEKEHGWAIKKMETEDQTVVQDCVPENFDPAPIQDEYAPVIFAQETVSHIVSVDMMSGEEDRENILRARASGKGVLTSPFKLLKSNHLGVVLTFAVYDTSLPPDATEEQRVEATIGYLGASYDMPSLVEKLLHQLASKQTIAVDVYDTTNTSGLIKMYGC",
    "response#3": "IPSAIDQRTFEEYTERTNFERPLTSGVAYALKVPHSEREKFEKEHGWAIKKMETEDQTVVQDCVPENFDPAPIQDEYAPVIFAQETVSHIVSVDMMSGEEDRENILRARASGKGVLTSPFKLLKSNHLGVVLTFAVYDTSLPPDATEEQRVEATIGYLGASYDMPSLVEKLLHQLASKQTIAVDVYDTTNTSGLIKMYGSEIGDISEQHISSLDFGDPSRNHEMI"
  },
  {
    "reference": "MIEFGDFYRLIAKGPLSPWLDTLPAQLSAWQRESLHGKFKTWFNAVEHLPQLTPTTLDLHSGVRAEMSPPISAGQREGMENMLRALMPWRKGPFSLYGLDIDTEWRSDWKWQRVLPHISPLAGRTILDVGCGSGYHLWRMIGEGAHLAVGIDPMQLFLCQFEAIRKLLGGDQRAHVLPLGIEQLPELAAFDTVFSMGVLYHRRSPLDHLYQLKNQLVTDGELVLETLVVEGDSQQVLVPGDRYAQMRNVYFIPSAPALKAWLEKCGFVDVRIADMAVTTTEEQRRTDWMTSESLAEFLDPHDHSKTVEGYPAPLRAVLIARKP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a high affinity for carboxy-S-adenosyl-L-methionine. The designed protein sequence is ",
    "response#1": "VFDLGSSLGSATLSIRRQIEGRQCQIIAIDNSQSMIERCEENLAAYVSDTQVTLVCDDIRNVDINNASMVVLNFTLQFLPPEDRDTLIKRIYDGMLPGR",
    "response#2": "DLGASLGAVTQALRRHVRIDGCKVIAVDNSPAMVARCSEYLHAQDAMFQELLPVEVIEADILALDLQPTSLVTLNFTLQFIPPERRLELLTRIRQALLPGV",
    "response#3": "VFDLGSSLGSATLSIRRQIEGRQCQIIAIDNSQSMIERCEENLAAYVSDTQVTLVCDDIRNVDINNASMVVLNFTLQFLPPEDRDTLIKRIYDGMLPGM"
  },
  {
    "reference": "MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLAELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGQQEGGHVSH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a specific function within the plasma membrane.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. For general function, the protein need meet that Required for efficient expression of pilin; Functions as a transient chaperone for propilin, preventing it from being prematurely degraded, and also promotes propilin translocation, positioning it in the membrane for processing to mature pilin. The designed protein sequence is ",
    "response#1": "FARSLATSYAAGVPLLDALGTVAKASGGELHQQAIQRLRQGMANGQGLNQAMAAEPLFPPLLVQLVAIGESSGTLDQMLEKAASHYEEQVSQALDQLTSLLEPAIVLVLGLLVGGLVVAMYLL",
    "response#2": "AARSLATQVTRARLEAIRRNAFVGLQVFTEGAGGYLLFVDQNANRRYDPGEEFGATHFGQGNWARVRLDPEKSALGNMPLLFDPRGIPAKPITATLVLTSGGATRKVVISQQV",
    "response#3": "LILLSLLLILAAIDAQTQLLPDGLTLPLMWAGLLFNLSATYVPLAEAVVGAMAGYLSLWSVYWVFRLLSGKEALGYGDFKLLAALGAWLGWQALPQTLLLAY"
  },
  {
    "reference": "MPNANVADLIVETLAQAGVGRIYGLVGDSLNAITESIRTRDDIDWVHVRHEEVAAFAAGAESQITGKLAVCAGSCGPGNLHLINGLFDCHRTRTPVLAIAAHIPSAEIGSGYFQETHPENLFRECSHYCELVSDPSQLPQVLEAAMRAAIGQGGVAVVVIPGDVALKEAPTRGIAPNAGLLPRKPIVRPAEPDLDALAELLNGSSRVTLLCGRGCAGAHADLLRLAENLKSPIVHALGGKEHVEYDNPYDVGMTGLIGFSSGYAAMMSCDVLLMLGTDFPYRQFYPTKAKIAQVDLRPENLGRRCRLDLGLVGDVGATIDALLPKLRAKTDRAFLDDSLKHYAKAREDLDELATPKPARGWFGLKRDRPVIHPQYLAKVVSETAADDAIFTADVGTPTVWAARYLKMNGRRRLLGSWVHGSMANAMAQAIGAQANDRGRQVVSLSGDGGFAMLMGDLLTLRQEGLPAKIVVFNNGSLGFVEMEMKAAGYIETGVALENPDFAAVARATGIHGVRVEDPAELESAARDVFAHDGPALLDVVVARNELSMPPKIDGQQVKGFSLYVMRAVMSGRGDSVLDLAKTNLLR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein have enhanced a ubiquinone + H2O + pyruvate = a ubiquinol + acetate + CO2.2. For general function, the protein need meet that It channels electrons from the cytoplasm to the respiratory chain at the cell membrane via ubiquinone.; A peripheral cell membrane enzyme that catalyzes the oxidative decarboxylation of pyruvate to form acetate and CO(2)3. The designed protein should have a high specificity for Mg(2+) binding, with minimal off-target effects.4. The designed protein should have flavin adenine dinucleotide binding, thiamine pyrophosphate binding, magnesium ion binding, pyruvate dehydrogenase (quinone) activity, ubiquinone binding within plasma membrane to regulate pyruvate catabolic process.5. The designed protein have Thiamine pyrophosphate enzyme TPP-binding, Thiamine pyrophosphate enzyme central domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "TVADQFAEILAAAGVRRIYGIVGDSLNGLTDAIRQQGKIEWIHVRHEEVAAFAAGAEAHLTGELAVCAGSCGPGNLHLINGLFDCHRSRVPVLGIAAQIPSGEIGSGYFQETRPQALFQECSHYCELVSGPNQMPRALEIAIRQAVGKRGVSVLVLPGDVALGQWSRAEGVQLAKLLNATQKVTILAGAGCAGAHTELIQLAGKLNAPIVHAMRGKEFIEYDNPFDVGMTGLLGFSSGYHAMMNCDLLLMIGTDFPYQQFFPTDATVVQIDLRGEQLGRRTKVDHGFVGDTKTTLRAYGVAGDSLNGITDSVRRHNGIEWVGVRHEETAAFAAGAEAGLTGKFAVCAGSCGPGNLHLINGLYDAHRSRVPVLAIAAQIPSQEIGSGYFQETHPEHLFKECSHFCELVSSPAQMPRMLEIAIQTALAKRGVAVVVLPGDIPHAVPADVGTPTVWAARYLRMNGKRRLLGSFTHGSMANALPQAIGAQMSHPGRQVISMSGDGGFAMLMGDLLTLKQHNLPVKLIIFRNDSLAFVELEMKAAGILDYGTDLYNPDFAKIAEGAGLLGLRAETPDQVEPMIAQALKHDGPALVETVADQFAEILAAAGVRRIYGIVGDSLNGLTDAIRQQGKIEWIHVRHEEVAAFAAGAEAHLTGELAVCAGSCGPGNLHLINGLFDCHRSRVPVLGIAAQIPSGEIGSGYFQETRPQALFQECSHYCELVSGPNQMPRALEIAIRQAVGKRGVSVLVLPGDVAL",
    "response#2": "DRLAALLDGAEAVTLLCGAGCAGAHDEVVALADLLAAPVVHALRGKEHVEWDNPFDVGMTGLIGFSSGYYAMESCDALLMLGTDFPYRQFYPTKAVVAQIDLRPEVLGRRTRLDMALVGDVGPTLRALARLAAPEPSPISRAADLLNAGRRVTILAGAGTAGAHDEVVALADALGAPIVHALRGKEFIEYDNPFDVGMTGLLGFASGYRAMDRADTLLMLGTDFPYRQFYPKHATTIQVDIRGEQLGRRHPVDLGLVGSVKDTAEAVRTIVAAWLPKGGARDATEILNSAAKVTILGGAGCNGAHKELIATADALKAPIVHALRGKEYIEYDNPFDVGMTGLLGFSSGYHAMEDCDVLLMLGTDFPYPQFFPSHAKIIQVDLRGNQIGRRTKVDLGLVGSIKDTLSALVSVVDPSLAERGFAEREAAGILNAAGKVTILAGAGVEGAHDEVVALADALAAPIVHSMRGKEFIEYDNPFDVGMTGLLGFSSGYRAMEDCDALLMLGTDFPYQQFFPSKAKIIQVDLRGEQLGRRVPLDLGLVGDVRDTAAARTVLAAWLPKGTDVGTPTVWAARYLTMNGQRRLLGSFNHGSMANALPQAIGAQVSHPRRQVISLSGDGGLAMLMGDLLSLRQLQAPVKVIVFKNNALAFVELEMKAAGFLDFATDLHNPDFAKMAEAAGLLGMTAEAPEQVRPMITQALRHDGPALVEDVGTPVIWAARYLGMNGHRRLLSSFNHGSMANALPQAIGAQISHPGRQVVTLSGDGGFSMLMGDLLSLRQLKLPVKVVVFNNGSLGFVELEMKATGFLKHGTDLVNPDFAKIAEGAGLFALHVDDPEEVRPALKQAMAHDGPALID",
    "response#3": "SIARQLAQTLEQAGVKRIWGLTGDSLNGLTDALHSMQSIQWMHVRHEEVAAFAAGAEAAITGQLAVCAGSCGPGNLHLINGLFDCHRSRVPVLAIAAQIPSSEIGLDYFQETHPQELFKECSHFVELVSHPAQMPEVLHRAMRSAILQRGVAVVVIPGDVALQEGLDAVNAAYRAPPLHPQFVTRTLDRLAAEDAVFTADVGTPVIWAARYLTMNGRRRLVGSFTHGSMANALPHAMGAQAAFPGRQVVALAGDGGLSMLLGELLTLQQNRLPVKLVVFNNASLNFIELEMKSVGFVNFGTGLDNPNFAAVAEAMGIKGVRVNDSSELEAGLAEALAHDGPALVDVATMREELSMPPSLTRTELAKVLTTSKKITILAGAGCAEAHEELIELAGRLKAPVVHTMRGKEFVEHDNPFDVGMTGLLGTSSGYQAMMTCELLLMIGTDFPYQQFYPRDATIVQIDRRGEQLGRRTKVDYGFVGDAKTTLLASVGRLVSRVAELLVDVLVEAGVQRVYGVSGDSLNGITDSIRTRKEIEWVHVRHEEVAAFAAGAEAHLTGKLAVCAGSCGPGNLHLINGLYDCHRSRVPVLAIAAQIPSNEIGSGYFQETLQRAADLLSRSRRTTLLVGVGAEGAKAEVLQLADTLGAPVVHSLRGKQHVEGDNPFDVGLTGLLGFASGYRAMERADTILMLGTDFPYRQFFPEHAKVIQVDVRGEQLGKRVPLEVGLVGDVRDTVREAGRRLAEAAAVLNGAENVTILAGAGVAGAHDEVVALAAKLQAPVVHALRGKEHIEWENPYDVGMTGLLGFASGYRAMERCDALLMLGTDFPYRQFYPAKARIVQVDIRGEQLGRRTPIDVGLVGGVRETAAA"
  },
  {
    "reference": "MPRPELPLPEGWEEARDFDGKVYYIDHTNRTTSWIDPRDRYTKPLTFADCISDELPLGWEEAYDPQVGDYFIDHNTKTTQIEDPRVQWRREQEHMLKDYLVVAQEALSAQKEIYQVKQQRLELAQQEYQQLHAVWEHKLGSQVSLVSGSSSSSKYDPEILKAEIATAKSRVNKLKREMVHLQHELQFKERGFQTLKKIDKKMSDAQGSYKLDEAQAVLRETKAIKKAITCGEKEKQDLIKSLAMLKDGFRTDRGSHSDLWSSSSSLESSSFPLPKQYLDVSSQTDISGSFGINSNNQLAEKVRLRLRYEEAKRRIANLKIQLAKLDSEAWPGVLDSERDRLILINEKEELLKEMRFISPRKWTQGEVEQLEMARKRLEKDLQAARDTQSKALTERLKLNSKRNQLVRELEEATRQVATLHSQLKSLSSSMQSLSSGSSPGSLTSSRGSLVASSLDSSTSASFTDLYYDPFEQLDSELQSKVEFLLLEGATGFRPSGCITTIHEDEVAKTQKAEGGGRLQALRSLSGTPKSMTSLSPRSSLSSPSPPCSPLMADPLLAGDAFLNSLEFEDPELSATLCELSLGNSAQERYRLEEPGTEGKQLGQAVNTAQGCGLKVACVSAAVSDESVAGDSGVYEASVQRLGASEAAAFDSDESEAVGATRIQIALKYDEKNKQFAILIIQLSNLSALLQQQDQKVNIRVAVLPCSESTTCLFRTRPLDASDTLVFNEVFWVSMSYPALHQKTLRVDVCTTDRSHLEECLGGAQISLA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must contain a signal peptide for proper functionality.2. The protein contains novel WW domains that confer a unique function or activity.3. A protein that can perform transcription coactivator activity in ruffle membrane, perinuclear region of cytoplasm, cytoplasm to support cell migration, regulation of intracellular transport, negative regulation of organ growth.4. For general function, the protein need meet that Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway; Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation; Plays a role in cognition and memory performance.5. The protein design should emphasize Acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "NMANLSLHDHDGSSGASDQDTLAPLPHPGAAPWPMAFPYQYPPPPHPYNPHPGFPELGYSYGGGSASSQHSEGSRSSGSNRSGSDRRKEKDPKAGDSKSGGSGSESDHTTRSSLRGPRERAPSERSGPAASEHSHRSHHSLTSSLRSHHTHPSYGPPGVPPLYGPPMLMMTPPPAAMGPPGAPPGRDLASVPPELTASRQSFRMAMG",
    "response#2": "NMANLSLHDHDGSSGASDQDTLAPLPHPGAAPWPMAFPYQYPPPPHPYNPHPGFPELGYSYGGGSASSQHSEGSRSSGSNRSGSDRRKEKDPKAGDSKSGGSGSESDHTTRSSLRGPRERAPSERSGPAASEHSHRSHHSLTSSLRSHHTHPSYGPPGVPPLYGPPMLMMTPPPAAMGPPGAPPGRDLASVPPELTASRQSFRMAMPDSILVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTPEMQVRIRQEIEKEKKVEPWKEQFFESYYGQSSC",
    "response#3": "DMLRETQISLGYINKVFCSKWLSSRQVVFGTKCNKLLVYDVNMRRVDAIPTLSNSRANHPEVQGGLHAIELSPSRSFLATGARNSSDIAVYRLPTLDPVCVGEGGHRDLIMGMCWLDDQFLVSGSKDSRMALWRINEDHMEFPDGGEEACPTFATIHPLSVKEVRTAQRIRSLCFNKEFKEIAALSLNGYIHIFNAETFKQTLSRKLPNCQDNVSIAYHSDGLYAVGCRSYTILLDARTLQTIKKITSRYSGCGIRATSFEGNLLTVGTGLGMLLFYDIRAGKYLESSVNASRTVALKCSKGIVYPEDEMDGFQQVKYVPAIYTHCYDSTRMRLFAAGGPLPATLVGNYAGVL"
  },
  {
    "reference": "VLSANDKSNVKAAWGKVGNHAPEYGAEALERMFLSFPTTKTYFPHFDLSHGSSQVKAHGKKVADALTKAVGHLDDLPGALSDLSDLHAHKLRVDPVNFKLLSHCLLVTLAAHHPSDFTPAAHASLDKFLANVSTVLTSKYR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a specific function within the hemoglobin complex. The designed protein sequence is ",
    "response#1": "EGLKVKLQWASAFGHAHQRVAFGLELWKGILREHPEIKAPFSRVRGDNIYSPQFGAHSQRVLSGLDITISMLDTPDMLAAQLAHLKVQHVERNLKPEFFDIFLKHLLHVLGDRLGTHFDFGAWHDCVDQC",
    "response#2": "EGLKVKLQWASAFGHAHQRVAFGLELWKGILREHPEIKAPFSRVRGDNIYSPQFGAHSQRVLSGLDITISMLDTPDMLAAQLAHLKVQHVERNLKPEFFDIFLKHLLHVLGDRLGTHFDFGAWHDCVDQL",
    "response#3": "EGLKVKLQWASAFGHAHQRVAFGLELWKGILREHPEIKAPFSRVRGDNIYSPQFGAHSQRVLSGLDITISMLDTPDMLAAQLAHLKVQHVERNLKPEFFDIFLKHLLHVLGDRLGTHFDFGAWHDCVDQL"
  },
  {
    "reference": "MAKELRFGDDARLQMLAGVNALADAVQVTMGPRGRNVVLEKSYGAPTVTKDGVSVAKEIEFEHRFMNMGAQMVKEVASKTSDTAGDGTTTATVLARSILVEGHKAVAAGMNPMDLKRGIDKAVLAVTKKLQAMSKPCKDSKAIAQVGTISANSDEAIGAIIAEAMEKVGKEGVITVEDGNGLENELSVVEGMQFDRGYISPYFINNQQNMSCELEHPFILLVDKKVSSIREMLSVLEGVAKSGRPLLIIAEDVEGEALATLVVNNMRGIVKVCAVKAPGFGDRRKAMLQDIAILTKGQVISEEIGKSLEGATLEDLGSAKRIVVTKENTTIIDGEGKATEINARITQIRAQMEETTSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGIVAGGGVALIRAQKALDSLKGDNDDQNMGINILRRAIESPMRQIVTNAGYEASVVVNKVAEHKDNYGFNAATGEYGDMVEMGILDPTKVTRMALQNAASVASLMLTTECMVADLPKKEEGVGAGDMGGMGGMGGMGGMM",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a low Km value for ATP + H2O + a folded polypeptide = ADP + phosphate + an   unfolded polypeptide..2. A protein that can perform ATP binding, isomerase activity, unfolded protein binding in host cell endosome membrane, cytoplasm, cell surface to support adhesion of symbiont to host cell, regulation of entry of bacterium into host cell.3. The ATP binding site should be stable and able to withstand changes in environmental conditions.4. For general function, the protein need meet that May play a protective role against the defense mechanisms generated by the infected macrophages. The designed protein sequence is ",
    "response#1": "KGGKVGLFGGAGVGKTVLMQELIHNIARFHEGYSVVVGVGERTREGNDLWLEMKESGVLPYTVMVYGQMNEPPGVRFRVAHTGLTMAEYFRDVEGQDVLIFIDNIFRFVQAGSEVSTLLGRLPSAVGYQPTLNTDVGEVQERITSTKKGSITAIQAVYVPADDITDPAPWSIFAHLDATTVRFEAEKSTSLYTQANVFAKMLKSEDDYNYDEKTKAVQLTEQGIDKAERMFKIDNLYDVNNVDVISHINTALRAHVTLQRDVDYMVNKGEVLIVDQFTGRTMPGRRFSEGLHQAISDKQGVDDYRRALDFCRQLRDGLHFRIFAADKLIQLTPAGRDHITNICGNLPGVWQVARARFELIEQALAAQLLYMRDKHYIVKDEKVQIVDEYTGRVMSDRTWESGLHQMIYSNKEIFLRGIQATGTTTLYTRTDRFAKTLTEDEDFKIDLESKTVSLTEDGIRKGEKYFGLKNLYDPDNMALNHHLDNALRANYIMLRDKDYVVRDGEVLIVDQFTGRIMDGRRYSDGLHQAIYSNKEIFLRYSNKEIFLRGL",
    "response#2": "KGGKVGLFGGAGVGKTVLMQELIHNIARFHEGYSVVVGVGERTREGNDLWLEMKESGVLPYTVMVYGQMNEPPGVRFRVAHTGLTMAEYFRDVEGQDVLIFIDNIFRFVQAGSEVSTLLGRLPSAVGYQPTLNTDVGEVQERITSTKKGSITAIQAVYVPADDITDPAPWSIFAHLDATTVL",
    "response#3": "KGGKVGLFGGAGVGKTVLMQELIHNIARFHEGYSVVVGVGERTREGNDLWLEMKESGVLPYTVMVYGQMNEPPGVRFRVAHTGLTMAEYFRDVEGQDVLIFIDNIFRFVQAGSEVSTLLGRLPSAVGYQPTLNTDVGEVQERITSTKKGSITAIQAVYVPADDITDPAPWSIFAHLDATTVRYGEANDSSSYYSADQFVRTLNNDDFLVDEESKAVTLTASGIEKANSFFRIDDLYNIEHSEKVHLIQNALRAHKVFKIDVEYIVKNNKIELVDAFTGRIMEGRSYSEGLQQAIAA"
  },
  {
    "reference": "WLMIVEKECRVIVMLAKCYEAGKKKCQKYWPDSKDTLTFGQIKVFNAEEVRYSGFLVRKFQIESVEKMITMEVFQYQYTNWPDHSVPYTTSNLVRMHKNVIQLLAEIGGDAPMVV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The desired protein should be able to perform H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] +   phosphate2. The designed protein must possess protein tyrosine phosphatase activity to support protein dephosphorylation under certain conditions.3. The designed protein have Tyrosine-protein phosphatase domains with specific sequence motifs that are crucial for its activity.4. The substrate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "WMMIVEQKCRVIVMLAKCFEAGKKKCQKYWPDSEETKTFGRVKVFNADEVKYCGFLRRRFHIESFDENDVCGRVFQYQYINWPDHSVPNTTSNLVRMHKYVIQCLEEIGGDAPMVC",
    "response#2": "WMMIVEQKCRVIVMLAKCFEAGKKKCQKYWPDSEETKTFGRVKVFNADEVKYCGFLRRRFHIESFDENDVCGRVFQYQYINWPDHSVPNTTSNLVRMHKYVIQCLEEIGGDAPMVV",
    "response#3": "WMMIVEQKCRVIVMLAKCFEAGKKKCQKYWPDSEETKTFGRVKVFNADEVKYCGFLRRRFHIESFDENDVCGRVFQYQYINWPDHSVPNTTSNLVRMHKYVIQCLEEIGGDAPMVC"
  },
  {
    "reference": "MRVPLSWLQDLVQLNETVEILAEGLSMAGFEVEAIDDLASHAQGVVVGLVKDHQPHPNADKLSVCQVDVGSGEGLQIVCGAPNVRIGIHVPVAMVGATLPAVGIKIKASELRGVPSQGMICSLAELGLESNGNGIAILEEIAENVPDLGQPVGPLLGLDDTVLELAITANRPDGMSMVGIAREVAALTGASLQLPQLDMAPIHKSFEPDSTSSASMLKGGLYGLTALENVDGELTSPAWLKQRLERSGCKSVNGVVDITNLVMLEQGQPLHAFDIDALEMITGQTVSAESFGLRQARNNELFNGLDGHQLQLNENCQIVTCHDIPIALAGVMGSAESGVSAKTRRVWLESAMFTPTAVRTTCRAVGLRTDASSRFEKGLPVEMTLASARRAVTLMEEHLGIKSNGCWVYGESPKSAEPVKLRREAIHRLLGPIGVENDNRYLEDDIIETSLLALGCELSPYNEGWLVIVPPSRRRDLSREVDLIEEVSRLVGFDRFEANLPDPLEPGGLTTAQTAERLLRQMLCGAGLQEVTTLSLVGADSDEPQRIAISNPLLAETSHLRTNLWEEHLRICQRNLQSSQPGCWLYEIGNVYTVTDELINQRAVLGGVICAERSFERWSTSGKIKSMTYHQARGQLSQVFQGLKLDINDRPLKDNHSLHPGRSAELFVEGKLLGDFGQLHPALSERLDLPEATYLFALDLQCIIQAATRSNRWNPTFRPFPTVPAMELDLAVIVSKECSCSDLIQAIRKAGKPLLEHVELIDRFEGGQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The desired protein should be able to perform ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) +   L-phenylalanyl-tRNA(Phe)2. The ideal protein would be one that plays a critical role in phenylalanyl-tRNA aminoacylation through magnesium ion binding, ATP binding, phenylalanine-tRNA ligase activity, tRNA binding within cytoplasm.3. The presence of tRNA-binding, FDX-ACB domains is critical for the interaction of the designed protein with its intended targets.4. Mg(2+) is required for the proper folding and stability of the protein.5. The protein should be able to bind Mg(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "IDVTEPTTRGQRGAQHPITILSEQIADIFVGMGWEIADGPEVEAEYFNFDSLNFIPDHPARTLQDTFHIAPEGSGQVLRTHTSPVQMRTMLSRDLPIYIACPGRVFRTDELDATHTPVFHQVEGLAVDKGLTMAHLKGTLDHLAKTLFGEEAKTRIRPNYFPFTEPSAEVDVWFADKKGGAGWIEWGGCGMVNPNVLIAAGVDPEEYSGFAFGMGIERTLQFRNGLPDMRDMVEGDVRFNTHDNLRVLMISRGFHEVFTWSFMSESIAEKFGYSNKLFIIDNPFNNNFNIMRPSIVPNLLQVTADNIVHGMSDLAIFEIGPVYDSLDQPKYSLSGIRTGNNLPRNHYNTDRKIDVFDAKADLIAALELFNVSYGNLTIERAEKEYYHPGKSGTLSFKNKATGYFGELHPNILDLFDIKQKVVGFEL",
    "response#2": "LSEQIADIFVGMGWEIADGPEVEAEYFNFDSLNFIPDHPARTLQDTFHIAPEGSGQVLRTHTSPVQMRTMLSRDLPIYIACPGRVFRTDELDATHTPVFHQVEGLAVDKGLTMAHLKGTLDHLAKTLFGEEAKTRIRPNYFPFTEPSAEVDVWFADKKGGAGWIEWGGCGMVNPNVLIAAGVDPEEYSGFAFGMGIERTLQFRNGLPDMRDMVEGDVRFTLDLTRTAYPYRVGTRHPISIVQKRICNIFFKLGFSIAEGPEIEDDWHVFSALNFAFDHPARDMQDTFFIKYDKNILLRTHTSSVQIRTMEKTQPPIRILCPGRVYRNEAISARAHCFFHQVEAFYINRNVSFADLRQVLVFFAKEMFDSGINTRLRPSFFPFTEPSAEMDIMCNLCRGKGCSFCKFTGWVEILGCGMIDPNVLDNCNIDSKTYSGYALGMGVERITSLKYQVKDLRLFSENDIRFLRQF",
    "response#3": "VDVTLPHDRVPAGARHPLTTLSERIEDIFVAMGYEVAEGPEAEAEWFNFDALNIGPDHPARGEADTFFVQGPEGGAESGVVLRTHTSPVQIRSALTRELPVYVICPGRVYRTDELDATHTPVFHQVELLAVDEGLTMADLKGTLDHMVQSLFGAEMKTRLRPNFFPFTEPSAEMDMLCYVCKGASVGNPDRPCRTCSSEGWIELGGCGMVNPRVLTACGIDPEKYSGFAFGFGIERMLMFRHNVEDMRDMVEGDVRFTRI"
  },
  {
    "reference": "MIVEDDIRVFSGTANVELTRKICQYINIKMGRAQVETFPDGETLVRIEDDVRGRDCFIVQPTCPPVNRHLMELLVFIDCLQRASANRITAVLPYFGYARQDRKHEGRTPITAKLVANLITTARANRVLAVDLHADQVQGFFDIPVDHLTAEPVLVEYFKSLNLKDKVLISPDVGNVKTANLYAQDLNGELAVIDKRRESGSRAVAQRIIGDVKDKDVLMFDDMITTAGTICSAAHLAQEHGSRSIRVGATHGIFAPPAAERLAAAPIDEIVITDTVPLTAEAKKLKNLRVLTVANLLGEVINRIHHHKSVSAIFERRL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P).2. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.3. The protein should be designed to localize specifically to cytoplasm in order to perform its role in 5-phosphoribose 1-diphosphate biosynthetic process more effectively.4. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MKLFSGNSVPKLAESIANKLYVNLGNASVGKFSDGEINVQINENVRGSDVFIIQSTCFPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSSRVPITAKVVADFFSSVDLHAGQIQGFFDIPTAPVLVRDIQARLKLDNVMVVSPDVGGVVRARALAKRIDAPLAIVDKRRERAGESEVMNIIGDVDGKICILIDDIVDSGGTLCNAADALRAQGASDVYAYITHGVLSGGAAAVYAIEHGFADHALPLLAEYVERTYSGQPLTVVAPDSGRVRVAERWTDRLGGCPLAFIHKTRDVTTPGQVVANRVVGEVAGRVCVVVDDEIQTGGTVCKAADLLFAAGAAEVIVVVTHPVLPDPAVERLKNSRISEVVVTNLAIEPGDKLADWTIALDLLANYVKEKYAGRELTVVAPDSGRVRVAERWTDVLGGTPLAFIHKTRDPLVPNQVVMNRVVGEVKGRTCILVDDMIDTGGTIAKAADALKEEGAADVVVAATHGVLSDPATERLKNGPISEVILTNALPLLAEYVERTYSGQPLTVVAPDSGRVRVAERWTDRLGGCPLAFIHKTRDVTTPGQVVANRVVGEVAGRVCVVVDDEIQTGGTVCKAADLLFAAGAAEVIVVVTHPVLPDPAVERLKNSRISEVVVTNL",
    "response#2": "MRVFTGSANPRLAEAVVRHLNIHLGKATVGRFSDGEVMVEILENVRGKDVFILQSTCAPTNDNLMEVMLMVDALKRSSAGRITAALPYFGYARQDRRPRSARVPISAKVVADMLMTAPVLINYLRERHPDTSNLTVVSPDVGGVKMARAYAEALGAELAIVAKHRVNATKVEAMNVIGEVEGRDVLLVDDMTETAGTLMAAADILRQSGAQRIYAGVSHAILGEMAHERIKNSAIEEVITTDAHPRAWMKALDLLANYVKEKYAGRELTVVAPDSGRVRVAERWTDVLGGTPLAFIHKTRDPLVPNQVVMNRVVGEVKGRTCILVDDMIDTGGTIAKAADALKEEGAADVVVAATHGVLSDPATERLKNGPISEVILTNAAPVLIRHMREKGLGNDNMVVVSPDVGGIKMTHAFSKALGSNFAIVAKHRTSATEVEALNVIGNVENCDVLLVDDMTETAGTLTAAAGLLKERGARRIFAAVSHALLNEKALARLAGSPIEELICTDALPGFAGKLIKLFSGTANPTLAASIARHLEMELGHVNVERFPDGEIFVQYIDNLRTADVFLIQPTCNPTNENIMELLIMLDAARRASAARITAVIPFFGYARQDRKDRPRVPITAKLVANLLTVAEENI",
    "response#3": "MKLFSGNSVPKLAESIANKLYVNLGNASVGKFSDGEINVQINENVRGSDVFIIQSTCFPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSSRVPITAKVVADFFSSVDLHAGQIQGFFDIPTRSFKNAAPVLARDVKEHYDLANLMVVSPDVGGVVRARALAKRLDCLLAIVDKRRDRPGESEVMNIIGDVEGKDCILIDDIVDSGGTLCNAAEAMLNMGAASVTAYITHGVLSGGAVTRVTSSKLRELVITDTNRLPVTIQYPYEKSI"
  },
  {
    "reference": "MDTIHDVFSAAQVREGERAVAKLLNIEMYALMERAGAAVFDCLQQVCPLASPSSSPLSSLPQASSIHTSTNPSSLPCVSSAQPPSLLVCCGGGNNGGDGYVVARLAQQAGYQVTVWQGSDPARLQGDAARAYHAWCAIGGETCLPEKAVPDGVGVIVDALLGTGLSGTVRETMAAMIAAINCASVPIIAVDLPSGLCADTGRVWGTAVQATHTVTFIGVKRGLVTGRAARHVGALHFAGLGVEKAFAQHCLSTEHLITTSRMDRLLPARDRCAHKGDHGRILLVGGDRGFGGAIRLAAEAAARSGAGLTAALTQSDNALAMLVAIPEIMAQGWQQDGAEAARQRLDWADVLVLGPGLGQSGWSQALYQTLSHTDKPLVLDADGLNLLARAPDYKNNRIITPHPGEAARLLQCSVAEVENDRFSAARALQHRYGGVVVLKGAGTLVDDGHHCWVCTAGNPGMATGGMGDVLSGIIGALLGQGLGLSDAAQCGVWIHSTAADHCAQDGERGMLASDLFPYIRQRVNRR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein contains novel YjeF N-terminal domains that confer a unique function or activity.2. The designed protein should be optimized for NADHX epimerase activity, metal ion binding activity in order to improve nicotinamide nucleotide metabolic process.3. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.4. The AMP binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MAAIDRETISMGEPGIDLMERAGQAIAEVVLERLDDADEGCGHDPGCGHDHGDDAGRGVLVLVGKGNNGGDGLAAARLLAAAQVPVTVLLLAPVAELSPDARRNHDRLPQAVTLLISSRRDWLTALTEAADGADLLVDAIFGTGIKPPLREEWSDLLRAINDLGLPCVAADVPSGVDGNDGRVDPVAVAADVTVTMGLPKLGLLLPAGREFAGQVLVADIGFPDEVCAGTDKHSRGHALVFGGYPMTGAARMAARGAARAGAGLTTLAVPEMALPIYAATLSSIMVRPWVTPEDFGHLLNASRFSAWLIGPGAGVGNGTFGHALAMLATGRPTVIDADAITAFQDDPGALDRAVQGPCVLTPHEGEFRRVFDPAGDKLTRTRAAALRCSAVVVLKGSDTVIAAPDGRAIINANAPPTLATAGSGDVLAGIILGLLAQGMTAFTAAAAGVWLHGAAAAEFGPGLLAEDLPDLLA",
    "response#2": "LLVVAGSRAFGGAAQLAGLGALRSGAGLITVAAPRCLEDPLRVGLPEALVVPLAETPAGTIAPAAPEVLEHLLEGRRAAAVGPGLADDPDTDTWVVQWLAGLDLPLVVDADALGAFARLEREPAFATDQVILTPHAGELGRLLGRPSAEVEAERLTLVGDLAVRWQCIVMLKGAPTVIGVPDGRVFINASGDDALARGGSGDVLTGLLVGLLAQGMDALDAALLGAYVHGLAGTHAARRQGNRSVLVREIV",
    "response#3": "VLVIGGSDVYSGAPALTGLAALRTGADLVRVLVPQAVVTPIRSYSPNLMVESLPTRVFDVTSVDMAVEAARGANVVSIGPGLGMAPETIRGVQDLLKRVLSLDMGVVVDADGLKALPGIGIKMDPSRCILTPHWGELQILLGQKSEPRPDIQERIRRAREAAAKYDSVILLKGPIDVIAHPDGRHKLNKTGVPAMTVGGTGDVLTGISSAMMARRRDAFEAAAAAAFVSGLAGEAAYQELGNHITATDCIAKIQ"
  },
  {
    "reference": "MEMREAYIERQTKETRIRVKLNLDGAGTAQVDTGIGFFDHMLEALARFGYLDLEVAAEGDLQVDPHHTIEDCGLVFGQAIREALGERRGIERVGDTLLPMDEALVQVALDLSNRPYLVWDVECPEGMVGGFPVEMAEEFFRAVSVQAGITLHIRLFSGKNRHHILEAIFKGFGRALGLALRENPRFQGVLSTKGVL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have imidazoleglycerol-phosphate dehydratase activity within cytoplasm to regulate histidine biosynthetic process.2. A protein should be able to manipulate the activity of enzymes in the Amino-acid biosynthesis, L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9, L-histidine biosynthesis pathway, either by activating or inhibiting their function.3. The protein should be designed to catalyze the following reaction D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-   4-yl)-2-oxopropyl phosphate + H2O. The designed protein sequence is ",
    "response#1": "MPITLAFLIIVLLLILLSIMYVSRARMPNLVPAIIVAVLGVVATLPFAHTSNPEAWTQVIESTKQFLGGHTDILIAVMLFGWAAAMMSKDGAFAVLANRIVKPLKHVKSLWLVTGLSGFVLALFTNDVALIIMTPIVLSLTRMLDAPQRSLVPYLFALASGTNLPSVATVTNIVANAELVQLGFSLSSYTARMFVPTALAVLVGSITLLWWFDRKQLPNFTLESTFTAEDEKIPDRKLAILSLVTVVILILLVFGVHPIWIAALLGAITVFILRRADANETVAKAFKSAPWDVVMFVAGIFVVIQGMVDVGIIDGLAKGITGLTGALGPWVAVFLTGIVASFFSAFVDNIPFATTMIPVVTALADAGVLPQSLAFGINSHHIIEATFGANNHHIAETCFVYYGELKLTMSDYFKTGVVMALVSLVIGYVVVSLLG",
    "response#2": "MXRVTKLESSKSGIKIAGKNIVVIDETLMDLSFXKKAIEXAKXAGISVVATTVNSSVAKLINXEAVKSMRXAGXXVIITDXPIXAAXITXGINSHHIIEATFGANNHHIAETCFQXPDXITVIGGCNXEKKXTXIXAXAVXVAXVADXVIXTNXSXXEDXIKXCPXIFXVTXTXAXAXAXAXNXXGXXVIXPXHLXAPXAXXXGX",
    "response#3": "MTTALVTGASKGIGAAIAVRLAADGFRVVVNYRSEKAAAEAVVAEIVAAGGEAIAVRADVSREDAVRGLFDAADAALGGLDVVVSNAGIAPVGYIADLTEDELDRTFAVNVRGQFFVAQAAHRHFNDGGRIIFLS"
  },
  {
    "reference": "MTMTSLSESLEASDPSKSAFLEFSHGYQSHQQHSPGVSHAHYPVHGLHQGAHSQYDAAFSPGAASYSRPLAYHYSTAHHHPGAYLPYQHNSAVGYSRVEDADSEKQSSIESGEIRLNGKGKKIRKPRTIYSSLQLQALNQRFQQTQYLALPERADLAAKLGLTQTQVKIWFQNKRSKYKKIMKHGSSGPEGEHLQAASASGAPCSPGMPPLWDVSMPSKGAPIHSGGYMNSFGHWYSGHHQDPMARTQMM",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that contributes to cell differentiation, regulation of transcription by RNA polymerase II by functioning in nucleus with RNA polymerase II cis-regulatory region sequence-specific DNA binding, DNA-binding transcription factor activity, RNA polymerase II-specific.2. The Homeobox domain within the designed protein should exhibit high affinity and specificity towards its target DNA sequence. The designed protein sequence is ",
    "response#1": "PRKQDIGDILQQIMTITDQSLDEAQAKKHALNCHRMKPALFSVLCEIKEKTGLSIRNTQEEEPVDPQLMRLDNMLLAEGVAGPEKGGGSAAAAAAAAASGGVSPDNSIEHSDYRNKLSQIRQIYHAELEKYEQACNEFTTHVMNLLREQSRTRPISPKEIERMVGIIHRKFSSIQMQLKQSTCEAVMILRSRFLN",
    "response#2": "MYPGTLRSPGGSSTAGVGTSGGSGSPLPASNFTAAPVYPHYVGYPHMSNMDPHGPSLGAWSSPYSPPREDWSTYPGPPSTMGTVPMNDMTSPVFGSPDYSTLGPTSGASNGGSLPDAASESLVSLDSGTSGATSPSRSRHSPYAWMRKTVQVTGKK",
    "response#3": "MEGLGGNVPSNQCRNFLSPSAFGAHHNSLSSGPAYTVGDRTHSVISESAKQCSPCPAPTSPPNPAISYGYHFGSSYYSCRNVGIQQTGLKSGAHASLAGYPVDKYMDVSSLI"
  },
  {
    "reference": "MSNGTGTPTAVVILAAGQGTRMKSDTPKMLHGIGGRTMLEHALHAARGVDPEQIVAVVGHQREKVVRAIAEWVGGIAEAEGDGALPGGVAIAHQEEQNGTGHAVQCALDDASLQEFEGTVLVTTSDVPLLDADTLSALLAEHNRKPRAAVAVLTSTAENPHGYGRIIRNSDGEVLRIVEEKDATDDERRTTEVNSGVYAFDAATLRHALTQLNTDNAQGELYLTDVIGIAREADRSVRGHRLDDAMVVAGVNDRVQLANLGAELNRRQCEKAMRGGATIVDPATTWIDVDVEIGRDVTILPGTQLHGATVIGDGATVGPDTTLTDVTVGEGATVFRVHGTESSIGANAQVGPFTFLRPGTVLGEDGKLGGFVETKKAVIGRGSKVPHLTYVGDAEIGEHSNIGASSVFVNYDGVNKHRTVIGSHVRTGSDTMFIAPLTVGDGAYSGAGTVIKDDVPPGALAVSGGPQRNIEGWVQRKRPGTPAAEAAEAAQAAAGDSTDAVAPGDGNPDTSA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.2. The designed protein should have a high specificity for Mg(2+), acetyl-CoA binding, with minimal off-target effects.3. The protein design should consider the endogenous proteins and enzymes present in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway to avoid interference with native metabolic processes.4. The designed protein must possess glucosamine-1-phosphate N-acetyltransferase activity, magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity to support regulation of cell shape, cell wall organization, peptidoglycan biosynthetic process, cell morphogenesis under certain conditions. The designed protein sequence is ",
    "response#1": "VVILAAGAGKRMQSALPKVLHTLAGIPLLMHVINAARLLSPDKICVVVGYGSDKVRQLTGSDDLIWVSQELQLGTGHALMQALPQLNTDGFTLVLYGDVPLVRIETLQQLIAIAGEKNCALLTAQQMPAGLALLTVNLENPTGYGRVVCQNGKVVAIVEEKDATPSQKQIKEVNTGIIVAPTALLSQWLPRLTNDNAQGQLIVDGVTLIDPDNSYIGPDVIIKQDAIIYPGCFIDGNTIIESDAVIGPNTEIRSCEVGENTTIRQSVAIDSKIGKRVQIGPFAHIRPQADIGDEVKIGNFVEIKKATLDKNSKVSHLSYIGDAEVGKGVNIGCGTITVNYDGKNKHLTKIEDDAFIGCNSNLIAPVTIGKGSYVAAGSTINKNVPADALSIARVRQNHLPGWQRPLKK",
    "response#2": "AVVLAAGQGTRMKSKLYKVLHPVMGKPMVQHVVNQLTKLKLENVITIVGFGAEKVKEQLGDVSNFVIQEEQLGTGHAVMQAEEFLKDKEGTTIVVCGDTPLLTSQTLEALLSHHDKEGAKVTVLTAKASDPTGYGRVIRNKYNDVERIVEHKDANESEITIDEINTGTYCFDNQSLFKALKQVSNNNSQGEYYLPDVIQILKKSNEKISAFQETDFAEGVNDRVALSQAEHIWQLRCRRQMMMSGVTMIAPETVFFSHDTFIEPDTVIEPHVFFGCGVRIENSVRIRAFSYLEGVHIGKNSAIGPFSRLREGSKIEKNVRIGNFCEVKNTTIGGGSKINHLSYVGDSMMGDNVNIGAGTVTCNYDGIHKSKTNIGDNAFIGSNSSLIAPITIGQGTYIASGSVITKDTPENSLVFARSRQVNKPGYADKIRDKARAKQRS",
    "response#3": "AVILAAGQGTRMKSKLYKVLHPVCGKPMVEHVIDHIGSLNVERIVTVVGHGAELVKETLGDKSEYVLQAEQLGTAHAVQQAEPILGDLEGTTLVVCGDTPLIRPETMQALFAHHEAQSAKATILTAVAENPTGYGRILRDSQGQVAQIVEQKDATQEQQRVKEINTGTYCFDNKALFEALKQVNNNNAQGEYYLPDVIEILQKQGEIVSAYETPDAHEILGVNDRVQLAEATRILRERINTKHMLGGVSLADANRFDVRGELHCGQDVFIDVNNVFSGKVVLGNGCSIGPNCILHNVTLGAHCEILANSVLEDCHIGESCHIGPFARLRPGTQLANDCKIGNFVETKKAVFGEGSKASHLSYLGDVTLGKEVNIGAGTITCNYDGANKHQTIIEDGVFVGSDTQLVAPVTVGANATIGAGSTIRKNVPANELTLTESKQRNIPGFAERFRNRK"
  },
  {
    "reference": "MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDTAINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGENFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDATFARVTAPLWQYLDVLHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKIASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAVWGDPSVLDPQKLPDGQRESLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be optimized for stability and expression in ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing where it will participate in transmembrane transport. The designed protein sequence is ",
    "response#1": "LITKTDTNPFFVKMKEGAAAKAKELGVTLKSYAGKIDGDSESQVAAIETCIADGAKGILIAASDTQGIVPQVQKARDAGLLVIALDTPLEPLDAADATFATDNLLAGKLIGQWAAATLGDAAKEAKVAFLDLTPSQPSVDVLRDQGFMIGFGIDPKDPNKIGDEDDPRIVGHDITNGNEEGGRTAMENLLQKDPTINVVHTINEPAAAGAYEALKSVGREKDVLIVSVDGGCPGVKNVAEGVIGATSQQYPLMMAALGIEAIKKFADTGQ",
    "response#2": "PGQVVALAGSSGGGKSTIAGLLERFYDISNGDITIDGYSIKQLNAKWLRSRIGIVSQEPSLFATTILENLRYGNPNATEDEIIEAAKLANAHQFISNFPKGYETIVGERGVQLSGGQKQRIAIARAILKNPQIIILDEATSALDSQSELLVQTALDNLMKGRTTLVIAHRLSR",
    "response#3": "LITKTDTNPFFVKMKEGAAAKAKELGVTLKSYAGKIDGDSESQVAAIETCIADGAKGILIAASDTQGIVPQVQKARDAGLLVIALDTPLEPLDAADATFATDNLLAGKLIGQWAAATLGDAAKEAKVAFLDLTPSQPSVDVLRDQGFMIGFGIDPKDPNKIGDEDDPRIVGHDITNGNEEGGRTAMENLLQKDPTINVVHTINEPAAAGAYEALKSVGREKDVLIVSVDGGCPGVKNVAEGVIGATSQQYPLMMAALGIEAIKKFADTGQA"
  },
  {
    "reference": "MAARVGAFLKNAWDKEPVLVVSFVVGGLAVILPPLSPYFKYSVMINKATPYNYPVPVRDDGNMPDVPSHPQDPQGPSLEWLKKL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization.2. For general function, the protein need meet that Complex I functions in the transfer of electrons from NADH to the respiratory chain; The immediate electron acceptor for the enzyme is believed to be ubiquinone.; Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis3. The designed protein should have NADH dehydrogenase (ubiquinone) activity within mitochondrial inner membrane, mitochondrial respiratory chain complex I, mitochondrion to regulate aerobic respiration, mitochondrial electron transport, NADH to ubiquinone, proton motive force-driven mitochondrial ATP synthesis, mitochondrial respiratory chain complex I assembly. The designed protein sequence is ",
    "response#1": "YSTQIMLFTIMEHPRTHHTTHKETKNITHPLARLMLTSILMGTMTKMSTLQTTTMVTMPKTIKLMALISTIIGVLLSKDLTHMTHHMKPKKPNKQNMFFNQLAFFNIPHRTITINTLKISQQTSTELMDLWTLEVWGPKGLSNTITNTIHLLTQQKNMIKNYMAIFTMTTMTVLLFT",
    "response#2": "YSTQIMLFTIMEHPRTHHTTHKETKNITHPLARLMLTSILMGTMTKMSTLQTTTMVTMPKTIKLMALISTIIGVLLSKDLTHMTHHMKPKKPNKQNMFFNQLAFFNIPHRTITINTLKISQQTSTELMDLWTLEVWGPKGLSNTITNTIHLLTQQKNMIKNYMAIFTMTTMTVLLFT",
    "response#3": "YSTQIMLFTIMEHPRTHHTTHKETKNITHPLARLMLTSILMGTMTKMSTLQTTTMVTMPKTIKLMALISTIIGVLLSKDLTHMTHHMKPKKPNKQNMFFNQLAFFNIPHRTITINTLKISQQTSTELMDLWTLEVWGPKGLSNTITNTIHLLTQQKNMIKNYMAIFTMTTMTVLLFS"
  },
  {
    "reference": "MAESASQAGGPDFPNVVLVGSRASGKSRASRKLAEAIGWTRISTDELLEARLGPIPAFVERSGWERFREEERRVLAGVTGERLIVDCGGGVVENPGSISRLRQLGTVYWIRAPAAVLRERLSRPKHRDSRPTLTGIAPAEEAALMLERRTPLYREAAESDVWTTASDGTAEEEAFETLLRAHFGPRLALTVAGAAVADLRAGLDRAIAESGPLDLIEVRLDILERPAPADLRVVLGDLPGPLLARLIATVRRRDEGGSFSGSEDDRVGLLAEAARLGAGYCDLEFEADRESGGAHARRIRSAAPAVRLVASVHDLNGVPAGFETLPDRIAPMRPALTKVAVRTAGRADVERVHSLIRAQASRETPIVGIAMGDAGTALRVTAGSAGAALSTYAPPEGLPAVAPGQLTAGEIRSRHCRWGRRLHALVPVYGVVGSPIGQSLSPPMHEAAFRRLGIDAAYLPFAVPPAELEDFLMAARLGGVGGLNVTIPHKQAVLPHLDSLDDDARRIGAVNTIIPMNGGLGGANTDWTGAVRALEEAVALPGRRATVLGAGGSARAVLHGLAHSGAEAVVISRSDRKAATLADEMGVAHAPAAALERIEGDILINTTPVGMAPDHDGCPAPAAAIAAHEIVFDLVFHPERTPLLRKAAGIGKRTVPGLRMLILQAAAAFERWTGRPAPVQVMADAARKARDAR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should take into account the physiological conditions of the Metabolic intermediate biosynthesis, chorismate biosynthesis pathway. The designed protein sequence is ",
    "response#1": "LSETLKEQSWFSTPRSAWEKVKAVRQVERPSASDYISHIFDFFVESHGDRAFSDDKAMIGGLAFLDGQPVTIIADEKGKDFKECQERNFGMPMPEGYRKALRLMKQAEKFNRPVISFVNTSGAFCGIEAEERGQGEAIARNLMEMSSLKVPVLCLMIGEGGSGGALATAVGNEVWMMENATYSILSPEGFASILWKDANRAKEASEVMNITAQDLKRLGVIEEIIPEFGGADHKTTEAIAGYMKEHIKDFLRAN",
    "response#2": "LSETLKEQSWFSTPRSAWEKVKAVRQVERPSASDYISHIFDFFVESHGDRAFSDDKAMIGGLAFLDGQPVTIIADEKGKDFKECQERNFGMPMPEGYRKALRLMKQAEKFNRPVISFVNTSGAFCGIEAEERGQGEAIARNLMEMSSLKVPVLCLMIGEGGSGGALATAVGNEVWMMENATYSILSPEGFASILWKDANRAKEASEVMNITAQDLKRLGVIEEIIPEFGGADHKTTEAIAGYMKEHIKDFLRE",
    "response#3": "LSETLKEQSWFSTPRSAWEKVKAVRQVERPSASDYISHIFDFFVESHGDRAFSDDKAMIGGLAFLDGQPVTIIADEKGKDFKECQERNFGMPMPEGYRKALRLMKQAEKFNRPVISFVNTSGAFCGIEAEERGQGEAIARNLMEMSSLKVPVLCLMIGEGGSGGALATAVGNEVWMMENATYSILSPEGFASILWKDANRAKEASEVMNITAQDLKRLGVIEEIIPEFGGADHKTTEAIAGYMKEHIKDFLRE"
  },
  {
    "reference": "MPSPALLCCCLVLLAGVGASRHQSTLSEDDCTHFPASLPHMLRELRAAFGRVKTFFQMKDKLDNILLTGSLLEDFKGYLGCQALSEMIQFYLEEVMPRAENHDPDIKDHVNSLGEKLKTLRLRLRRCHRFLPCENKSKAVEQVKSAFSKLQEKGVYKAMSEFDIFINYIETYMTMRMKI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation ; Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha2. The protein must contain a signal peptide for proper functionality.3. The protein design must be focused on enhancing the efficiency of negative regulation of membrane protein ectodomain proteolysis. The designed protein sequence is ",
    "response#1": "GSHSLRYFHTAVSRPGLREPRFIIVGYVDHTEFVRYDSDAENPRYEPRARWMEREGPEYWEQQTQIVKGHEWSFRVSLRNLLGYYNQSEGGSHTLQVMSGCDMGSDRSLRRGYEQYAYDGRDYISLNEDLKTWSAADLAAQITLNKWDQAGELDYYKAYLEGPCLEWLLRYLEHGKETC",
    "response#2": "GSHSLRYFHTAVSRPGLREPRFIIVGYVDHTEFVRYDSDAENPRYEPRARWMEREGPEYWEQQTQIVKGHEWSFRVSLRNLLGYYNQSEGGSHTLQVMSGCDMGSDRSLRRGYEQYAYDGRDYISLNEDLKTWSAADLAAQITLNKWDQAGELDYYKAYLEGPCLEWLLRYLEHGKETS",
    "response#3": "GSHSLRYFHTAVSRPGLREPRFIIVGYVDHTEFVRYDSDAENPRYEPRARWMEREGPEYWEQQTQIVKGHEWSFRVSLRNLLGYYNQSEGGSHTLQVMSGCDMGSDRSLRRGYEQYAYDGRDYISLNEDLKTWSAADLAAQITLNKWDQAGELDYYKAYLEGPCLEWLLRYLEHGKETL"
  },
  {
    "reference": "MNRVVALVVAAGRGRRFGGDIPKQYLDLGGRPVLRHSLAALAANPQVTAVRAVIHPDDRDLYDACAQGLDLLEPVAGGETRQDSVRLGLESLAGLEPQAALIHDGARPFLDGGIVARVIGALAEHPGAIPAIAVADTLKRGADGLVAATVERAGLWRAQTPQGFRFADILAAHRACAGHELTDDAAVAERAGLAVALVEGSEDNVKITTAADLERGRRVFQGPGEFRTATGFDVHRFAPGTGVWLCGVLVPHDSALDGHSDADVAMHALTDALLGTIAAGDIGHHFPPSDPRWKGVASRRFLAHAAALVAGLGGRIVNVDVTILCERPKIGPHRAAMQSALSEILGLAHDRVGIKATTTEGLGFTGRREGIAAQASATVWLPR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should consider the endogenous proteins and enzymes present in the isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6, isopentenyl diphosphate biosynthesis via DXP pathway, Isoprenoid biosynthesis pathway to avoid interference with native metabolic processes.2. The protein should have a low Km value for 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-   erythritol 2,4-cyclic diphosphate + CMP.3. The designed protein must possess metal ion binding, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity to support isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway under certain conditions.4. The designed protein should have a binding site that is specific to the target 4-CDP-2-C-methyl-D-erythritol 2-phosphate, a divalent metal cation ligand. The designed protein sequence is ",
    "response#1": "MRVGTGFDVHRLADGEDLWLCGVKIDHHQGLAGHSDADVGLHAVVDAILGACARGDIGDHFPPSDPQWKGAASSAFVQHAVKLAGEAGYAIGNVDLTLICEAPKIGPHRGAMAVRLAELLGIPVSAVSVKATTTEKLGFTGRGEGIAAQAVTTLVEVPDAADVVAAGGRVGTGFDVHRLADGEDLWLCGVKIDHHQGLAGHSDADVGLHAVVDAILGACARGDIGDHFPPSDPQWKGAASSAFVQHAVKLAGEAGYAIGNVDLTLICEAPKIGPHRGAMAVRLAELLGIPVSAVSVKATTTEKLGFTGRGEGIAAQAVTTEVWRF",
    "response#2": "MRVGTGFDVHRLADGEDLWLCGVKIDHHQGLAGHSDADVGLHAVVDAILGACARGDIGDHFPPSDPQWKGAASSAFVQHAVKLAGEAGYAIGNVDLTLICEAPKIGPHRGAMAVRLAELLGIPVSAVSVKATTTEKLGFTGRGEGIAAQAVTTLVEAADAILARAGTPRTGNGYDVHSFEKGDCLILCGIKIPFHKKLNGHSDADVALHALTDAILATQGAGDIGTHFPPSDPQWKNVSSEIFLRHALDLVTQAGGRIANVDITLIAEEPKIGPYRQTMTENLMRILTISSNRISIKATTNEKLGFIGRGEGIAALATASGPILRVAGDDRVGQGYDVHQLVKGRPCIIGGVTLPYEYGLLGHSDADVLIHAIIDALLGAAGLGDIGQLFPDDDPRFKDSDSRQLLQTVSGLLASKGYRVGNIDATLLAEQPKMHPYLKEMQKNLAIDCQIGIERVNVKATTSEKMGFVGRQEGIAAMAICLPRTGWGYDVHRYLADDEEDAKLLAKARPMILGGVPIPRAPRVIAHSDGDVLLHALTDALLGIACAGDIGQLFPDTDAANENMPSAVFVVEALRRVRACGYRPVHADLTVVAQTPKLAAHKARMAAHIAQLLELPAGSVNVKATTEEGLGFTGRKMGLKAVAVVTAMG",
    "response#3": "MRVGTGFDVHRLADGEDLWLCGVKIDHHQGLAGHSDADVGLHAVVDAILGACARGDIGDHFPPSDPQWKGAASSAFVQHAVKLAGEAGYAIGNVDLTLICEAPKIGPHRGAMAVRLAELLGIPVSAVSVKATTTEKLGFTGRGEGIAAQAVTTTVEVVARPVSEANVKITNPADLALLAPAHCAAPRTGWGYDVHRYLADDEEDAKLLAKARPMILGGVPIPRAPRVIAHSDGDVLLHALTDALLGIACAGDIGQLFPDTDAANENMPSAVFVVEALRRVRACGYRPVHADLTVVAQTPKLAAHKARMAAHIAQLLELPAGSVNVKATTEEGLGFTGRKMGLKAVAVVTATVDVVARPIS"
  },
  {
    "reference": "MSHVAHSLKNLLRDRVAIPSVLDVWVHGLSADSRRVRPGDAFIALAGSGGDPWAHVDDAVANGAVAVLLEDGAGDPREYRGAVLVPVAGLADCLAGLADYFHGHPSAHLTVIGVTGTNGKTSVTGFLAAILEAGGIPAATMGTLGYAFRESRETASHTTPDVTRVHSLLAGFLDQGAKAVVMEVSSHALVQGRVDRVRFDAGVFTNLSPEHLDYHADMAAYGAAKARLFMDHEPDIAVVNADDDFGRQLLGQLPAETRLWRYGSMAQQLECRIRDARPASDGMAAIFDTRLGRLSLQTRLMGHFNIENLAAASATALALGIPPGVVETAAAGVEAPPGRLEPHAATSGLKLVIDYAHTAAALEHALAALRPHVSGELWCVFGCGGNRDQTKRPAMGEVAERLADHVILTDDNPRDESPEGIVADILSGMADAAGARVIHDRDRAITTAFREADATDLVLIAGKGHETTQERGGVRTAFSDAGVVRRLIGEGGEAA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The presence of Mur ligase central, Mur ligase C-terminal, Mur ligase N-terminal catalytic domains is critical for the interaction of the designed protein with its intended targets.2. The ideal protein would be one that plays a critical role in regulation of cell shape, cell division, cell wall organization, peptidoglycan biosynthetic process through ATP binding, magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity within cytoplasm.3. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.4. The designed protein should contain at least one Meso-diaminopimelate recognition motif motif to achieve its intended function.5. The designed protein should have a binding site that is specific to the target meso-2,6-diaminoheptanedioate, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP ligand.6. The protein should have a low Km value for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "MQLKDLLQPLKISRIIGSGDIDEITGITCDSRQVKEGFAFVCIVGAKSDGHDYAPAAIDAGAAVIVAERDMTLDSRAAAAGDLFVAVVGHKTDGRHYIPQAIAQGVAAIVAQADADTPDGTRVEMHGVPVIYLANLNQRLSALAGRFYGNPSSKLHLIGVTGTNGKTTVTNMIAHILEEGQKPVGLIGTIEIRTGSQVLSAVTGTNGKTSVTYMLKKIIEKAGFKAGLIGTIQNMIGDEIIAAHNTTPNAYELNSLFALMKAKGCTYVIMEVSSHALDQSRVYKLDFEAAMFTNLTQDHLDYHITMDNYLNAKKKLFKMCKTAIINSDDSYAPELMKDLDCRIVTYSTGNDSTYSAKGINYHPASVDYEFVSDSTIEHIHVNTGGKFTVYNSLCAACCGACVALGASLDDIKEALLQVETIPGRFQYLRSTSGITAIIDYAHTPDALLNVLKTIENIRTKNETVFTVVGCGGDRDKTKRPEMAAIACHHSDKVILTSDNPRSEDPNEILKDMMAGVDPVGLRKTLSIVDREQAIKTAVSMAEADDIILIAGKGHETYQEIKGEKIPFDDRKIVRDILRLQQE",
    "response#2": "MVTGTNGKTSTAWWLAQALEGSAFGPCAVAGTLGIGVPPAVDYNGLTTPDPVTLHRALRDLVARGVKACAMEASSIGIAERRLDGLHFDVAVFTNFTQDHLDYHGDMAAYWAAKAELFRWPGLRAAVVNIDDVQGARLAGELLDTALVELWTCSAVGAPARLSAVEAGHGPQGLRFQVVEQGGAEAMVSTTLVGDYNVANLLGVIGVTGTNGKTTIATVLYNMFRRMGYKCGLCSTVCNYIDGRAIPTECTTPDAVTLNQLLGQMADEGCEYAFMEVSSHSVAQHRIGGLTFAGGIFTNLTRDHLDYHKTFENYRNAKKAFFDALPAGAFAITNADDRNGMVMVQNTRARVKTYSVRGAADFRGRVLEESFEGMNLEIDGHEVSVQFVGRFNVSNLLAVYAVLLALGFDADAVADALGESTGVPGRMQRVERGQDFLAIVDYAHKPAALESVIATLRGHLAADTAGRLAVVVGAGGDRDAAKRPLMGAAGARGADLLIVTDDNPRTEDPAAIRAAVRSGALEVPAAERGEVREIGDRAEAIAAAVAWARPGDAVLIAGKGHEVGQVVGAEVLPFDDVEVAAAALRARAGELGAVQ",
    "response#3": "MGVRGVTLASTEVRPGDLYAALPGSKTHGVRYLPDAVERGAVAVLTDPTGQEQVPAGGLPVCVVDDPRAVLGDVAARVYGRPSDRLMVLGVTGTNGKTTVTNMIAHILEEGQKKVGLMGTIHNRIGQKILPGSLTTPDCLELAGLFAEMAAAQMDYAVMEVSSHALAQNRVAGVEFDLGVFTNLTQDHLDFHGTMEEYLAQKSRLFSGLDPLGKKGDQKMAILNRDDPSCDYLSDRCRVPFVTYGLDKRSMVRAENVQLSSQGIQYDLKYINKMSPIRMKVHGQFNVYNSLAAIAMTLDSRAAAAGDLFVAVVGHKTDGRHYIPQAIAQGVAAIVAQADADTPDGTRVEMHGVPVIYLANLNQRLSALAGRFAGIEPAAAVAGIGSAPGVPGRMEVFVAENRPTVVVDYAHTPDALEKALQAARLHCHGTLWCVFGCGGDRDKGKRPLMGAVAEQLADRVIVTDDNPRSEDPHAIVTDILSGLLDAGRAQVIAGRAEAVTSTVMQASAQDVVLVAGKGHETGQEVAGVIHPFDDRDELRAALARWMKG"
  },
  {
    "reference": "MKAYAKKRISYMPSSPSQNVINFEEIETQKENILPLKEGRSAAALSKAIHQPLVEINQVKSSFEQRLIDELPALSDPITLYLEYIKWLNNAYPQGGNSKQSGMLTLLERCLSHLKDLERYRNDVRFLKIWFWYIELFTRNSFMESRDIFMYMLRNGIGSELASFYEEFTNLLIQKEKFQYAVKILQLGIKNKARPNKVLEDRLNHLLRELGENNIQLGNEISMDSLESTVLGKTRSEFVNRLELANQNGTSSDVNLTKNNVFVDGEESDVELFETPNRGVYRDGWENFDLKAERNKENNLRISLLEANTNLGELKQHEMLSQKKRPYDEKLPIFRDSIGRSDPVYQMINTKDQKPEKIDCNFKLIYCEDEESKGGRLEFSLEEVLAISRNVYKRVRTNRKHPREANLGQEESANQKEAEAQSKRPKISRKALVSKSLTPSNQGRMFSGEEYINCPMTPKGRSTETSDIISAVKPRQLTPILEMRESNSFSQSKNSEIISDDDKSSSSFISYPPQR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The intended protein structure should align with strand-helix-helix-turn criteria.2. A protein that contributes to mitotic spindle assembly checkpoint signaling, cell division, positive regulation of protein autoubiquitination, negative regulation of ubiquitin-protein transferase activity, mitotic DNA integrity checkpoint signaling by functioning in mitotic checkpoint complex with protein kinase activity.3. The protein must exhibit the following characteristics: Component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis; Component of the mitotic checkpoint complex (MCC) which inhibits the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20..4. The presence of BUB1 N-terminal domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "LSVGSRKEHGTALYQVDLLVKISSEKASLNPKIQACSLSDGFIIVADQSVILLDSICRSLQLHLVFDTEVDVVGLCQEGKFLLVGERSGNLHLIHVTSKQTLLTNAFVQKANDENRRTYQNLVIEKDGSNEGTYYMLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTS",
    "response#2": "LSVGSRKEHGTALYQVDLLVKISSEKASLNPKIQACSLSDGFIIVADQSVILLDSICRSLQLHLVFDTEVDVVGLCQEGKFLLVGERSGNLHLIHVTSKQTLLTNAFVQKANDENRRTYQNLVIEKDGSNEGTYYMLLLTYSGFFCITNLQLLKIQQAIENVDFSTAKKLQGQIKSSFISTENYHTLGCLSLVAGDLASEVPVIIGGTGNCAFSKWEPDSSKKGMTVKNLIDAEIIKGAKKFQLIDNLLFVLDTDNVLSLWDIYTLTPVWNWPSLHVEEFLLTTEADSPSSVTWQGITNLKLIALTASANKKMKNLMVYSLPTMEILYSLEVSSVSSLVQTGISTDTIYLLEGVCKNDPKLSEDSVSVLVLRCLTL",
    "response#3": "LLKNNRFEEAEKFAMAFMLDVQKVYKGHVHYLMDSCDESDESFETLMTKMGQIQDHNMVAETYFTLVGMSRRCDRIRTYLTHAKKRRITDLDILKMIEALCYTWGTYRIIAGPEENEPSRPQVNETIWDLFVEALHDANPWIEIYNEFISDAQFTQARVIFSRHGKSITDYMCDDEENTISRLETLFRMFIDAISSDISKWSNVIEHVTTDILPACVMITEELIPCLESLITSLISLLEYRDSTNWPENAI"
  },
  {
    "reference": "MKYQNSLAFAREQDAEDPLKKYRNEFHIPKDKDGNDWLYFTGNSLGLQPKKAQYYVNLEMKDWAEQGVEGHFNGTYPWVTYPETLQGMMAKVVGAKPEEVIIMDTLTTNLHLLMVSFYQPTAKRHKILIESDAFPSDRYAVQSQIKFHGYDPETSLVEWSPKEGKDLLDMDDFESILEEQGEEIALILIGGVNYYTGQRFDFKKLADLGHAKGCYVGIDLAHGVGNIMPDLHNSGVDFSAWCTYKYLNSGPGSLSGIFVHERHAHNRELPRFAGWWNHNKETRFNMRLPFDVMPGAEGWQLSNPPILSLVPIRAALEMFEEVGMEQLREKSVKLTGFLQYLMGELGSDVVRVITPRNPEERGCQLSISVNNADKRLHEQLMENHVITDWREPNVIRCAPTPFYNTFEDVYQFVQILKGLIKKS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that contributes to anthranilate metabolic process, tryptophan catabolic process, quinolinate biosynthetic process, 'de novo' NAD biosynthetic process from tryptophan, L-kynurenine catabolic process by functioning in cytoplasm with pyridoxal phosphate binding, 3-hydroxykynureninase activity.2. The protein should have a high affinity for pyridoxal 5'-phosphate.3. The designed protein must have at least one Aminotransferase class V domain.4. We are looking for a protein with broad substrate specificity for H2O + L-kynurenine = anthranilate + H(+) + L-alanine.5. A protein that is capable of catalyzing reactions within the quinolinate from L-kynurenine: step 2/3, Cofactor biosynthesis, NAD(+) biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "QLLAEQASEQMCKIVGASPEEVIAMGTLTMNLHLLLASFYKPSETKHKILLDWKAFPSDHYAIESQIAWHGLDPKNSMVFLGPDDGEFVISTSKILAAIDEHADETALLLLPGIQYYTGQLFDIPTITEYARARNIIVGWDLAHAYANVELKLHDWNVDFAAWCTYKYGNAGPGSMGGLFVHGQDVATHAIKEQTAKILGALPTEVVIMETLTANLHLLMSSFYRPTKDRWKIIIEGKAFPSDHYAVLSQIANHSLDPSALITIDPPSASSPYLSNEHILSVISRHAATTALVLLPGIQFYSGQFFDIELITRHCQSLGITVGWDLAHAAGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHGDDVATHEKLSERVAKIVGAQGNEVMVTGSITVNIHQMLATLFHPTAEKSVIVVDELNFPSDIYAVESHLRLRGLDPAVSMRKVKSTDGYTLTLDAIEEMLTEDVAILMLPSVLYRSGQLLDLRRITEMAHAKGILVGFDLAHSIGSE",
    "response#2": "QLLAEQASEQMCKIVGASPEEVIAMGTLTMNLHLLLASFYKPSETKHKILLDWKAFPSDHYAIESQIAWHGLDPKNSMVFLGPDDGEFVISTSKILAAIDEHADETALLLLPGIQYYTGQLFDIPTITEYARARNIIVGWDLAHAYANVELKLHDWNVDFAAWCTYKYGNAGPGSMGGLFVHLTAIKEQTAKILGALPTEVVIMETLTANLHLLMSSFYRPTKDRWKIIIEGKAFPSDHYAVLSQIANHSLDPSALITIDPPSASSPYLSNEHILSVISRHAATTALVLLPGIQFYSGQFFDIELITRHCQSLGITVGWDLAHAAGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHE",
    "response#3": "EKLSERVAKIVGAQGNEVMVTGSITVNIHQMLATLFHPTAEKSVIVVDELNFPSDIYAVESHLRLRGLDPAVSMRKVKSTDGYTLTLDAIEEMLTEDVAILMLPSVLYRSGQLLDLRRITEMAHAKGILVGFDLAHSIGSG"
  },
  {
    "reference": "MSQDLAYKHDAVYPSGLSLDELAALLAQEPPYRANQAFAWFAKGVLSFEAMTNMPQRLRTRLSEYFDGHTRSSTVEKSLVDEDGSLKLQIRLRDGAAVECVLLEDIEGRKTACLSSQVGCPMGCTFCKTGGLGFLRNLGPDEILEQFHHLRDARGFISNVVFMGMGEPLLNLDNVRKAITILSHPKGIGMSMRKITISTSGIVPGILDIANAGPFVRLAVSITSANPNLRTQLMPVNKTWPLADLKAALLEYQRNTHERITLEVVLLGNVNTHENDALELADWIHPLKAQVNLIAWNPIAGMPFETPTARQVRLFSEVLERKGIVTVQRMSRGRGVMGACGQLGDTLKTTREH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a binding site that is specific to the target [4Fe-4S] cluster ligand.2. The protein should have tRNA binding, rRNA binding, tRNA (adenine-C2-)-methyltransferase activity and be active in cytoplasm.3. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. The designed protein sequence is ",
    "response#1": "SSQAGCGMNCPFCATGQAGLTRNLSTGEIVEQVAGGQRALNRGDIAGGPGRVSNIVFMGMGEPLANYKAVLGAVRRITEPSPSGLGISQRGVTVSTVGLVPAIHKLTEEGLSVRLAVSLHAPDDELRDTLVPINTRFKVREVLDAAFGYARKSGRRVSIEYALIKDVNDHAWIG",
    "response#2": "SSQAGCGMNCPFCATGQAGLTRNLSTGEIVEQVAGGQRALNRGDIAGGPGRVSNIVFMGMGEPLANYKAVLGAVRRITEPSPSGLGISQRGVTVSTVGLVPAIHKLTEEGLSVRLAVSLHAPDDELRDTLVPINTRFKVREVLDAAFGYARKSGRRVSIEYALIKDVNDHAWISPNL",
    "response#3": "DQDRITLCVSTQVGCRMGCAFCATAKIGFIRDLTAGEIVRQIMEVNEHLATNSEKVTNIVFMGMGEPLDNYHNVVKAIGIITDEMGLGYSHRKVTVSTSGVVDRIDELFKLKKQVNLAVSLNATTDDIRSAIMPINKKFNIEKLMKKLKSLPIQKRKRITIEYVMIKGVNDTLDDAKRLVRLLNGLPIKINLIAYNDGGNENYRAPDEQTVLSFQKYLVDKHITAFIRKSLGKH"
  },
  {
    "reference": "MKKIMSDRATGSETTHFGFEDIALRDKQGRVNRVFEEVASNYDLMNDLMSAGLHRAWKSSLITELSLPRGARRFRLLDVAGGTGDIALRALKRGGPGVHVTLFDINPAMLDVGRARTKEAGFEKRVEIVEGTAEELPFPEHSFDAVTISFGLRNVPQMDKALAEARRVLAPGGRFFCLEFSTVDVAVFDKIYDTYSFKVIPQIGKHVAGDEDAYRYLVESIRKFPPPDDLAHMMRTAGFERVSYTPYTGGVAALHSGWRL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity, S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity to facilitate ubiquinone biosynthetic process, aerobic respiration, methylation.2. The protein design should consider the endogenous proteins and enzymes present in the menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2, Quinol/quinone metabolism, menaquinone biosynthesis pathway to avoid interference with native metabolic processes.3. The protein have enhanced a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "MSNKSFGFREVPQDEKTRRVGAVFSSVASKYDLLNRLLSFGIDRRWKDALVWWLNPPSGRPLRLLDVAGGTGDIAFRALRRGGGRAQVTVCDINAAMLAVGRDRAIDAGLSDNLTFVEGNAEALPFPERSFDAYTIAFGIRNVPRIPLALAEAHRVLKPGGWATVLEFSHVDMPLLDRLYDLWSFRVIPPLGQLVAGDAASYRYLVESIRKFPRQADFAAMIRDAGFARVTWRNLSGGIAALHSGWKI",
    "response#2": "MTPDAPAARPAPARAAARAPARPARPARPAGPARPAGTARPAETARPSAAARPAGTAQPSAAARPATTAQPAAAPAAAAPAAAARPAAAARPAVAVRPAVAARPAEAARPAALPRPARPAGPAATAAAGTAGAARASGAPRASGALRASGALRASGAAGASGAAGASGAAGASGAAGAGGASGASGASGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSGVSG",
    "response#3": "MERLSGKVALVTGAGKGIGRGIAVKLAEAGAKVIVNYSSREEAAENLVKEIRASGGQAVGIRADVSKPEDVERLLKAAVNEFGRLDIVVNNAGILRDTFLMRMSEEEWDSVININLKGVFLCSQAAARVMTKQKSGRIINIAYDLLNRLLSFGIDRRASYCASKGGLIMLTRAMALELGPHNILVNAIAPGFIESKMTAGMTEEDRKQMVEAIPMKRLGKPEEVARAVAFLASEDASYITGEIIYVDGGMTL"
  },
  {
    "reference": "MASVSPLAKFKLVFLGDQSVGKTSIITRFMYDKFDTTYQPTIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSSVAIVVYDVSNRQTFLNTSKWIEDVHRERGQSNVIIVLVGNKTDLVEKRQVSISEGEDKGKEYGVMFIETSAKENFNIKALFRKIAAALPGVDSYSLATKSDDMVDVNLKTTSNSSQGEQQGGAGGGGGCSC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design must feature the Effector region motif.2. The designed protein should have a binding site that is specific to the target GTP ligand.3. A protein that can perform GTPase activity, GTP binding in plasma membrane, cytosol, Golgi apparatus, Golgi membrane, endomembrane system to support retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum, intracellular protein transport, intra-Golgi vesicle-mediated transport.4. The protein must exhibit the following characteristics: Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER) .. The designed protein sequence is ",
    "response#1": "REVFRVLVSFIDFGKQKFSDNIKAMALRFINTALEVSGSHISDFPSLRVLITDTLCKSLLQLIRSDHTALLTNSLLVMTTLLQAMPGSLKLQQELFISYLISCLHIPSTTPC",
    "response#2": "RKAIIIEGAELFNESPSDGIAFLTQHSIIKQSDNPTCIVEFFHSTNRLSKRVLGEFLTKGSNSHILNAFISAFDFKGKRIDEALRLLLQSFRLPGESQLIERVLETFSHYYMSANPDSMSSKDAAFVLSYSIIMLNTDQHNPNIKSQRRMTLDDFCRNVRGVNDGQDFDRNFLSEIYKAIKENEC",
    "response#3": "RKAIIIEGAELFNESPSDGIAFLTQHSIIKQSDNPTCIVEFFHSTNRLSKRVLGEFLTKGSNSHILNAFISAFDFKGKRIDEALRLLLQSFRLPGESQLIERVLETFSHYYMSANPDSMSSKDAAFVLSYSIIMLNTDQHNPNIKSQRRMTLDDFCRNVRGVNDGQDFDRNFLSEIYKAIKENEC"
  },
  {
    "reference": "MARIAGINIPPQQHAEIGLTAIFGIGRTRARKICEAAGVPVTKKVKDLTDAELERIREHIGVFAVEGDLRREVQLSIKRLIDLGTYRGMRHKRGLPVRGQRTRTNARTRKGPRRAAASLKK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein localizes to the ribosome.2. For general function, the protein need meet that Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA The designed protein sequence is ",
    "response#1": "ME",
    "response#2": "MPSRLDNVVYRLGFAVSRRQARQLVRHGHITVNGRRVDLPSYRVRPGDEIAVAEKSRNLELIRQNLS",
    "response#3": "MSRLDNVVYRLGFAVSRRQARQLVRHGHITVNGRRVDLPSYRVRPGDEIAVAEKSRNLELIRQNLI"
  },
  {
    "reference": "MGFKVKVEFLGGLDVISNKVREHSLKVPLEEGEATVKDLIELITKSIIADPKDIPVFIEDDTVRPGILVLINDTDWELEGMEEYVLESGDVFTFTSTLHGG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein should be able to manipulate the activity of enzymes in the tRNA modification, 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis pathway, either by activating or inhibiting their function.2. The protein should be optimized for stability and expression in cytosol where it will participate in tRNA wobble uridine modification, tRNA thio-modification, protein urmylation.3. For general function, the protein need meet that Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln); Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation; Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as AHP1; The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U; The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.; Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by the MOCS3 homolog UBA4 The designed protein sequence is ",
    "response#1": "METSGSEPGREPAQAGGPRALPGAAEPGAAALGEPEPGPAPAAETGAAAAAPAPAPGPEPGSAPSGAASAAASAPAPGPEPVPASSVAAAAAAAAPAASAPGPEPAASSAAAAAAPAAAAAPAAAAAPSAAPGPVPASSAAAAAAPAAAAAPAAAAAPSAAPGPVPASSAAAAAAPAAAAAPAAAAASSAAPGPVPASSAAAAAAPAAAAAPAAAAAPSAAPGPVRASSAAAAAAPAAAAAPAAAASAAPAPGPEPVPASSAAAAAAPAAAAAPAAAAAPSAAPGPVRASSAAAAAAPAETAAAEPAQAVPAPSAAAEPAQAVPAPSAAAEPAQAVPAPSAAAEPAQAVPAPSAAAEPAQAVPAPSAAAEPAQAVPAPSAAAEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPAQAVPAPSAAEEPA",
    "response#2": "MSQFSTFSTLARGHHLSGGALEATVGGRSDTALVATSGPVFPGGTRLAPTLGPKALLPPALLPTAAAGAGSLLSPSALSTLGPLAAASPLTGMLSPLAAGLPPAAASPLTGALNPLAAGLPPAAASPLTGALSPLAAGLPPSAASPFTSGLSPLGAGLSPTAASPYSSPFSPFPGSPLSPFSASPFSPLGPGGPLSPSAASPFGSPFSPLGAASPFSPTSPFGAPSPFGQGSPFGSPFSPFGAASPFGASPSPFGAAGSSPFASGASPFASSASPFGSPLSPFGAASPFGAASPLASPFGAASPFGAASPLASPFGAASPFGAASPFAASPFGAASPFGAASPLASPFGAASPFGAASPFAAASPFGAASPFGAASPFGAASPLASPFGAQSPFGAASPFGAASPFGAASPFAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGAASPFGA",
    "response#3": "MASGSPPHRPIHGDGLSPLPSPTSFARSFPPTASTDSSSLSSSSLSPPSSPSSSASFSPRSPASLPSDAKPALSPRAPASYRARRRNAPPPTPPAAPKAKKRKGRPPLGNQTKKTRSASDPPSAGAGTFFGSAPAPGKKRRRGGGAAAPSLRAPAAPREGGGARGGEAAGAQKRRRRGGGAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPTPAPAPAPAPAPAPAPTPAPAPAPAPAPTPAPAPAPAPAPAPAPVPAPAPAPAPAPAPAPAPAPVAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPVPAPAAAAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAAAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPARAPAPAPAPAPAPAPAAAAPAPAPAAAAPAPAPAPAPAPAPAAPAPAPAAPAAPAPAAAAPAPAPAAPAAPAPAAAAPAPAPAPAAATEP"
  },
  {
    "reference": "MALLSEELSSILPNPDFDDEEEDYVERETSHADERQIGVKFHIPPYGQRKGWFPSSPEDFGDGGAFPEIHVAQYPLDMGRKRSAKSAGNTLALQVTSSGAVDYNAIARQGHEHGELVQASFRDLIPLRARLGVGEISLEKPSDEQKQEVANKTKLALQKILSKQIAQSQPKSAVVQQRDDPVYIRYTPSNQMGQALSKQRIIKMVTAEQDPMEPPKFRHKKVPRGPPSPPPPVLHSPPRKVSAQEQQDWQIPPSISNWKNPKGYTIPLDKRLAADGRGLNDVEINDGFAKFSEALYTVERQAREEVRYRAIMRQKMAEKEKQEKEQRLFMLAQKAREDRMGRNAASSGPSHAKPRSTSVSSEERSRSRAGSFSHHSESENEDEDSEAFRRRQELRRERRRQAEKDLRLSRMGAEKRAKLAEKDRPRDVAERVALGLSKPSMSSDTMIDSRLFNQASGLGSGFQDEDSYNVYDKPWRAAPSSTLYRPGATLSRQVDASAELERITSESRYDVLGNAHKKFKGSDEVVESRAGPVTFEKDIADPFGVDTFLNNVSSKKT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be optimized for stability and expression in spliceosomal complex, post-mRNA release spliceosomal complex where it will participate in mRNA cis splicing, via spliceosome.2. For general function, the protein need meet that Involved in pre-mRNA splicing. The designed protein sequence is ",
    "response#1": "MPKFYCDYCDTYLTHDSPSVRKTHCSGRKHKENVRDYH",
    "response#2": "PSICEQCLTDADLRMTRAARGAECKICTLPFTLYHFKPPGAPRVTKTLVCRRCAAQRNVCQCCMLDLAWKLPVALRDELVSLVQGSDERTPEASNEMVRRFLALRGGQLGGARLF",
    "response#3": "EYGTSDLVREVYGLAVETLGEDFMDEKLFIAYARFETKLKEYERARAIYKYALDRLPRSKSITLHKAYTTFEKQFGDREGVENVILAKRRVQYLR"
  },
  {
    "reference": "MDSHQELSAGSPISYDFLDPDWCFKRYLTKDALHSIETGKGAAYFVPDGFTPILIPNSQSYLLDGNSAQLPRPQPISFTLDQCKVPGYILKSLRKDTTSTERTPRPPNAFILYRKEKHATLLKSNPSINNSQVSKLVGEMWRNESKEVRMRYFKMSEFYKAQHQKMYPGYKYQPRKNKVKR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be optimized for transcription coactivator activity, RNA polymerase II cis-regulatory region sequence-specific DNA binding activity in order to improve cell differentiation, positive regulation of induction of conjugation with cellular fusion. The designed protein sequence is ",
    "response#1": "LTDEELVTMSVRELNQHLRGLSKEEIVQLKQRRRTLKNRGYAASCRVKRVTQKEELEKQKAELQQEVEKLASENASMKLELDALRSKYEALG",
    "response#2": "STTDPLWRSCHEYDNEMVITVNGRRIFPTLEYTVTGLDTFKLYSMCMHLDLVDDKKLRFTGGQWAESVSTEKKDPPRKVWHHNGSQTGKDWMLRNVSFDQIRITNRKSKEDGNASYVHLLTQHRYIPVLTIYEGDQLVHTARIPHSQFISVTAYHKGELNTLKTNNNPYSTGSRR",
    "response#3": "STTDPLWRSCHEYDNEMVITVNGRRIFPTLEYTVTGLDTFKLYSMCMHLDLVDDKKLRFTGGQWAESVSTEKKDPPRKVWHHNGSQTGKDWMLRNVSFDQIRITNRKSKEDGNASYVHLLTQHRYIPVLTIYEGDQLVHTARIPHSQFISVTAYHKGELNTLKTNNNPYSTGSRR"
  },
  {
    "reference": "MARRISPVVAFLLCFGLSHFFEAEARLQHSVALHRQKREWIVPPQILEENVDYTKKDFIAKIRSDKEVAHMKYLRYSLRGVGADQEPFNLFVVNPETGYVRITGILDRESISQYNLSGIALFTDGSIAENDIGLRIKVKDQNDNAPVFGVMNPGAVDELSAVGTEVMRLNCFDADEPGNPNSQIKYEIVDQQPAGQSMFTVENNRRVVVANPNLDRETVDQYVLLVKASDLNGAPGGNAATGTVTIKINDVNDNVPTLGGPYEASIEENTEKVEVMRLKVSDLHLKGTDNWEGDCYIASGNEAGYFSIHMDPKTNEAVLMLDKAVDYEDVKDLNLGIGVANKAPFHPSVSGGSQGATISFGGSGGGAGSGAAGGAGAMGGASGSGGGTGASSWSSSGVPLYNVNIKVKNQPEGPKFFPGTKAIPISEGKAFDSTEIIARYPAIDTDTGKEATNVKYIKSSDPDNWLTIDEKTGAIRMNKAPDRESKYLVNGTYYAKVLSITQDLPSKTATGTIAIQVEDFNDHCPTLISNVQTLCTDKDAVLVTAKDEDAPPNGAPFDFNIVSEGTAGKWTVEYMNDTTAIFRTHEKLWPGPHELMVEVTDQQGLKCPEPQKLQVDVCTCKNQGGCDRTATGDAKKGSRLGPAGIGLLLLALLALLLIPLLLLLCTCGMTGAFTDMPFETKVHLISSNTEGQGEDRDVPLMCPPSNVDGMGFMTKDYMAVGAMHSAGLGLGVGAGVGAAGFLESTSTMGGRGYNEMELDYMNSIGRNN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Extracellular topological domain of the protein should be designed to be flexible or rigid.2. The protein should be optimized for stability and expression in desmosome where it will participate in convergent extension involved in gastrulation. The designed protein sequence is ",
    "response#1": "LFTNEEHSDMHAYIAMFGTVTLLCTFFTLATFLADWKNSNRYPAVILFYVNACFFMGSIGWLAQFMDGARKEIVCKSDNTMRLGEPSSTETLSCVIIFSLVYYSLMSGVIWFVMLTYAWHTSFKALGTTHQPLSGKTSYFHMVTWSIPFILTVSILAIAEVDGDSVSGICFVGYKNYRYRAGFVLAPIGVVLVVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGIFGFLAFGFVFITFGCHFYDFFNQAEWEKSFREYVLCEANVTIAHQTNKPIPECTIKNRPSLLVEKINLFSMFGTGIAMSTWVWTKATILIWKRTWCR",
    "response#2": "PLFTNEEHSDMHAYIAMFGTVTLLCTFFTLATFLADWKNSNRYPAVILFYVNACFFMGSIGWLAQFMDGARKEIVCKSDNTMRLGEPSSTETLSCVIIFSLVYYSLMSGVIWFVMLTYAWHTSFKALGTTHQPLSGKTSYFHMVTWSIPFILTVSILAIAEVDGDSVSGICFVGYKNYRYRAGFVLAPIGVVLVVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGIFGFLAFGFVFITFGCHFYDFFNQAEWEKSFREYVLCEANVTIAHQTNKPIPECTIKNRPSLLVEKINLFSMFGTGIAMSTWVWTKATILIWKRTWCKIIGA",
    "response#3": "LFTNEEHSDMHAYIAMFGTVTLLCTFFTLATFLADWKNSNRYPAVILFYVNACFFMGSIGWLAQFMDGARKEIVCKSDNTMRLGEPSSTETLSCVIIFSLVYYSLMSGVIWFVMLTYAWHTSFKALGTTHQPLSGKTSYFHMVTWSIPFILTVSILAIAEVDGDSVSGICFVGYKNYRYRAGFVLAPIGVVLVVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGIFGFLAFGFVFITFGCHFYDFFNQAEWEKSFREYVLCEANVTIAHQTNKPIPECTIKNRPSLLVEKINLFSMFGTGIAMSTWVWTKATILIWKRTWCV"
  },
  {
    "reference": "MTRYIFITGGVVSSLGKGLASAALGALLQARGFTVRLRKLDPYLNVDPGTMSPFEHGEVFVTDDGAETDLDLGHYERFTGVAARKTDSVSSGRIYSNVLEKERRGDYLGKTIQVIPHVTNEIKDFLSTGEGEVDFMLCEIGGTVGDIEGLPFFEAIRQFGQDRPRGQCIFVHLTLLPYIAASGELKTKPTQHSVKELRSIGIAPDVLVCRSEGPIPQKEREKLALFCNVRPESVIAAQDLKSIYDAPLAYHAEGLDQAVLDAFQISPAPRPVLTKWQDVSDRVHNTDGEVRIAIVGKYTQLEDAYKSVSEALTHGGMANRVKVRTEWLDAEIFEKDDAPARLEGFHGILVPGGFGERGTEGKIKAAEFARTRNIPYLGICLGMQMAVIEAARHLADMENAGSEEFDDEKGKKRFTPVVYHLKEWVQGNHRVERSKGDDKGGTMRLGAYTAYLAEGSRVAGVYGDTVIEERHRHRYEVDMSYRKQLEEQGLLFSGVSPDGRLPEIVEVKDHPWFIGVQFHPELKSKPFAPHPLFRDFVRAAKDLSRLV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The desired protein should be able to perform H2O + L-glutamine = L-glutamate + NH4(+)2. The designed protein should have a high specificity for UTP, Mg(2+) binding, with minimal off-target effects.3. A protein should be able to manipulate the activity of enzymes in the Pyrimidine metabolism pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "LHDAYLSVSEAVKHGGFANNARVDLRWVASDTCETPEGAQKALEGVDAIIVPGGFGIRGIEGKLGALRWAREHKIPTLGLCLGLQCMVIEAARDLLGLTGASSTEMDPETPDPVVHTMDSQVDIVEGDGDLGGTMRLGAYPAVLQEGSVVAEAYGKTQVSERHRHRFEVNNDYRDQLESVGLHISGTSPDGNLVEFVELDRSMHPYYVATQAHPEFKSRPTKAHPLFAGLVK",
    "response#2": "VEDAYLSIKEALKHAGIESGCTVSITWVEAENLEDDLKTVRCLEQVDGILVPGGFGVRGAEGKIKAIRYARENDVPFLGLCFGMQLAVIEFARNVVGLENANSSELEDTPHPVIDILPEQDGVEEMGGTMRLGNYDATLKEGSLAHRIYGTTRIVERHRHRYEVNPKYIDRIEAKGMIFSGKNKNRMEILEMPAHKFFFASQFHPEFTSRPGKPSPPFRAFVES",
    "response#3": "VEVALVGKYINLHDAYLSVSEAVKHGGFANNARVDLRWVASDTCETPEGAQKALEGVDAIIVPGGFGIRGIEGKLGALRWAREHKIPTLGLCLGLQCMVIEAARDLLGLTGASSTEMDPETPDPVVHTMDSQVDIVEGDGDLGGTMRLGAYPAVLQEGSVVAEAYGKTQVSERHRHRFEVNNDYRDQLESVGLHISGTSPDGNLVEFVELDRSMHPYYVATQAHPEFKSRPTKAHPLFAGLL"
  },
  {
    "reference": "MGSEALVHVFLVSFPGQGHVNPLLRLGKRLAAKGLLVTFSTPESIGKQMRKASNITDEPAPVGEGFIRFEFFEDGWDEDEPRRQDLDQYLPQLELIGKDIIPKMIRKNAEMGRPVSCLINNPFIPWVSDVAESLGLPSAMLWVQSCACFCAYYHYYHGLVPFPSEAEPFIDIQLPCMPLLKYDETPSFLYPTTPYPFLRRAILGQYGNLDKPFCILMDTFQELEHEVIEFMSKICPIKTVGPLFKNPKAPNSVRGDFMKADDCLEWLDSKPPQSVVYISFGSVVYLTQKQVDEIAFGLLQSGVSFLWVMKPPHKDAGLELLVLPDGFLEKAGDNGRVVQWSPQEQVLAHPSVACFVTHCGWNSTMESLTSGMPVVAFPQWGDQVTDAVYLVDVFKTGVRMCRGEAENRVITRDEVEKCLLEATVGPKAVEMKQNASKWKAAAEAAFSEGGSSDRNIQAFVDEVRARSVAITGKSTANGVALDLAEKSAEINGKVDLVETKTNGKVELVEA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Has the highest activity with 3,4-dihydroxybenzoate, vanillate and gallate in vitro; Glucosyltransferase that catalyzes the formation of 1-O-beta- D-glucose esters with hydroxybenzoic acids as preferred glucosyl acceptors.2. The protein must have high specificity towards gallate 1-beta-glucosyltransferase activity.3. The protein should have a high affinity for UDP-alpha-D-glucose, an anthocyanidin.4. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of 3,4-dihydroxybenzoate + UDP-alpha-D-glucose = 1-O-(3,4-   dihydroxy-benzoyl)-beta-D-glucose + UDP. The designed protein sequence is ",
    "response#1": "MADKKRIAFVMVGLGGYLPERSMRDRDHSALEIQAACYKAVKELGYEPILFTTNQADAMEHVNVFRDPNVELCEKLIKEHNLFIVENGLHVGHEPSKDAIRAMKPKVVIDCMLPGVDTISQVLGTDRVSFCGFNNCSFPVLQQHIFYTLHALYGFLPDDLQVNLAGNCWAPQEEVLAHPAVGGFFTHCGWNSTVEAVSEGVPMICHPRHGDSFGEASPKCIVNDDAQFMQGVIEVLEKLL",
    "response#2": "MTTKQNKLETFDYSSVEEAVEKIRAGKTIVVVDDEDRENEGDFIVAAEKVTPEIINFMASHGRGLICAPLTEERCEELELDLMVGKNTALHETAFTVSVDLIGHGCTTGISAHDRAKTIQALIDPSSKPEDLGRPGHIFPLRAKEGGVLRRAGHTEAAVDLARLAGFEPAGVLVEIMNEDGTMARVPQLKEVAEKFNLKLISIADLIEYRMKHEKLIKREIAVEVAELPTKMGTYFYLTKKEKMGHNLEHEENKCLNLKIQHDPHEIPHNGTSYEYLKTKKEKMGHTLQHEEEMWCAQFQVLKHPSVGCFMTHCGWNSTLESIACGVPMIGFPKMFDGQDRADIIRTNSILLKMGEELHF",
    "response#3": "MEIVKVEEKKIAEKETAIANGVGCLSHGLGCCASSLSTVPGSCALKIVTGPCCCGPKLRAGCGCGPTCGCGLT"
  },
  {
    "reference": "MAEAFKFELVSPERLLVSEQVESVVIPGAEGEMTVMAHHAPVMTTIKPGVVTVKTASGGEERYVVFGGFADIVPAGCTLLAESAVAVKDVDRADLARRIQEAKEDAADAKDDQARSKAEQFLSQLTTLEGAILPA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that localizes to plasma membrane, proton-transporting ATP synthase complex, catalytic core F(1) and can perform proton-transporting ATP synthase activity, rotational mechanism, ATP binding with high specificity and efficiency.2. For general function, the protein need meet that Produces ATP from ADP in the presence of a proton gradient across the membrane. The designed protein sequence is ",
    "response#1": "VNPLDRDREWTVSVKVKLGDRVKPGQVVAEVPETSIVTHRVMVPPHLSGEVVEVVPNGKYTVDQEIVTVKDDKGNNHQIRLHQKWPVN",
    "response#2": "GQRVFDTFFPLGMGGTAAIPGGFGAGKTMTQHQLAKWSSADIIVYVGCGERGNEMTDVLEEFPKLEDPSTGKSMMERTVLIANTSNMPVAAREASIYTGITIAEFYRDMGYNVALMADSTSRWAEALREISGRLEEMPAEEGFPAYLPSRLAEFYERAGYVKTLGQDREGSISLIGAVSPPGGDFSEPVTQNTKRYVRCFWALDKSLASARHFPAVNWLESYHGWLKENINEDWIKLRNMAMELLKEEDRLQEIVKLVGEDVLPDNQRLILEVARLIKIGFLQQNAFSKVDRFATPEKQYWMLKIIMFLYEKARPLIKNH",
    "response#3": "GQRVFDTFFPLGMGGTAAIPGGFGAGKTMTQHQLAKWSSADIIVYVGCGERGNEMTDVLEEFPKLEDPSTGKSMMERTVLIANTSNMPVAAREASIYTGITIAEFYRDMGYNVALMADSTSRWAEALREISGRLEEMPAEEGFPAYLPSRLAEFYERAGYVKTLGQDREGSISLIGAVSPPGGDFSEPVTQNTKRYVRCFWALDKSLASARHFPAVNWLESYA"
  },
  {
    "reference": "MESVDNIIVFSGSSNPDLTQKICDYVGIPVGKTKVEPFPDGETLVKLEQDVRGRDCFVVQSTCPPVNNNLVELLIFLDCLHRASALRITAVLPYFGYARQDRKSEGRTPISAKLVANLITTARADRVLTVDLHAEQVQGFFDIPLDHLKAEPVLVKYFRKLELQDKVLVSPDVGNVKTAKIYAQDLGGELAVIDKRRISGSQAEAVRIIGDVKDKHVLMFDDMITTAGTICSAARLVKDKGAKSVRVGATHGIFAPPALERLSEAPIDEIVVADTIPLSREAGQLQKLKVVSVASLLGEAIIRIHQNRSISAIFESF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.2. The designed protein should have ATP binding, magnesium ion binding, kinase activity within cytoplasm to regulate ribonucleoside monophosphate biosynthetic process, nucleotide biosynthetic process, phosphorylation.3. The designed protein should have a high specificity for D-ribose 5-phosphate binding, with minimal off-target effects.4. The designed protein should interact with enzymes and substrates within the Metabolic intermediate biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "AMPVLLEHMRRELDPSTLTVVSPDMGRVRVADIWSDKLGVPLAIIHKRRDPLVPNQVSVHEIVGDVSGRVCLLVDDLIDTGRTIVKAAEALKASGATGVVVAATHAVFSDPAVELLQSEFIDSVVVTDAAPVMIGHYQDNPLPNLTVVAPDTGGAERARAYAKRLGADLALCDKRREKANVAEVMNVVGDVRGRSCLIVDDMCDTGGSLTKVARALKENGAGRIHACFTHPVLSGNACEKLAASEIEQIVVTNL",
    "response#2": "AMPVLLEHMRRELDPSTLTVVSPDMGRVRVADIWSDKLGVPLAIIHKRRDPLVPNQVSVHEIVGDVSGRVCLLVDDLIDTGRTIVKAAEALKASGATGVVVAATHAVFSDPAVELLQSEFIDSVVVTDP",
    "response#3": "AEYFAQKQIEDLTVVSADEGFAKKARKLADRLNVPLAIIEKRRLGNEGITEAMGIIGNVAGRNALIVDDEIDTAGSITQAVRVVREQGARDIYCSATHGIFSGPAIERLRAAPLKELVITNAR"
  },
  {
    "reference": "MSTAERAPILLTPGPLTTSYRTRRAMMVDWGSWDSDFNELTASVCQRLLKIVGGEGSHTCVPLQGSGTFAVEAAIGTLVPRDGKVLVLINGAYGKRLAKICEVLQRPFSTLETEENVPTTAADVERLLAADPAISHVALIHCETSTGILNPLEAIAKVVERHGKRLIVDAMSSFGAIGIDARKVPFDALIAASGKCLEGVPGMGFVFARSAALEASAGNCHSLAMDLQDQHAYMRKTGQWRFTPPTHVVAALHEALSQYEEEGGLPARQRRYASNCETLLGEMARLGFRSFLPAEIQAPIIVTFHAPRDPRYRFADFYQRVREKGFILYPGKLTQVETFRVGCIGHVDAAEMRQAVAAIGEALRELEVLEI",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be optimized for 2-aminoethylphosphonate-pyruvate transaminase activity activity in order to improve nitrogen compound metabolic process, organic phosphonate catabolic process.2. The protein should be designed to have high stability and activity towards (2-aminoethyl)phosphonate + pyruvate = L-alanine +   phosphonoacetaldehyde.3. For general function, the protein need meet that Involved in phosphonate degradation. The designed protein sequence is ",
    "response#1": "MQGSGTFSVEAAIANVVPRDGKVLVPQNGAYCQRILKICKVLGRAHVELPIPEDRPATAAAIEAALKKDPSITHVAQVHCETGAGVLNPLPEIAAVCARLGKGLIVDAMSSFGAIEIDARTMPFDALVAATGKCIEGVPGMGFVLVKKTVLEGSQGNSHSLALDLYDQYTYMQKTTQWRFTPPTHVVAAFRTALDQFLEEGGQPVRGERYRRNYETLVQGMAVLGFRPFLSPDVQAPIIVTFR",
    "response#2": "MQGSGTFSVEAAIANVVPRDGKVLVPQNGAYCQRILKICKVLGRAHVELPIPEDRPATAAAIEAALKKDPSITHVAQVHCETGAGVLNPLPEIAAVCARLGKGLIVDAMSSFGAIEIDARTMPFDALVAATGKCIEGVPGMGFVLVKKTVLEGSQGNSHSLALDLYDQYTYMQKTTQWRFTPPTHVVAAFRTALDQFLEEGGQPVRGERYRRNYETLVQGMAVLGFRPFLSPDVQAPIIVTFR",
    "response#3": "MQGSGTFSVEAAIANVVPRDGKVLVPQNGAYCQRILKICKVLGRAHVELPIPEDRPATAAAIEAALKKDPSITHVAQVHCETGAGVLNPLPEIAAVCARLGKGLIVDAMSSFGAIEIDARTMPFDALVAATGKCIEGVPGMGFVLVKKTVLEGSQGNSHSLALDLYDQYTYMQKTTQWRFTPPTHVVAAFRTALDQFLEEGGQPVRGERYRRNYETLVQGMAVLGFRPFLSPDVQAPIIVTFR"
  },
  {
    "reference": "MRIVVSGGGTGGHIYPALAMIREIERRTPCEVLYIGTENGLEADIVRRAGIPFESIEISGIRRSLSFENVKTGFRFLKSVVRVRKLLRDFQPDIVVGTGGFVCGPVLYTAAKMGYKTLVHEQNSLPGITNKFLARYVDRVALSFKGSGHHFGKNKGKTILIGNPRASEVAMLQIDPVEEKRKYGFEQDRPLIVVYGGSRGAPAINKAVVDMIPKLTETDWSLLFVTGQVHYEDIQAQLGTLPDRIQLRPFIYDLPLILKASQLVISRSGASTLAELTTLGLPSILIPSPYVTENHQEVNASSLVETGASLLIRESELTGDRLFDACTKAIADQADMSKASLALGMPNAASDLVDELLRLIQRKN",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be designed to have high stability and activity towards di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-   alanine + UDP-N-acetyl-alpha-D-glucosamine = di-trans-octa-cis-   undecaprenyl diphospho-[N-acetyl-alpha-D-glucosaminyl-(1->4)]-N-   acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-   D-alanyl-D-alanine + H(+) + UDP.2. For general function, the protein need meet that Cell wall formation; Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II).3. The designed protein should have a specific function within the plasma membrane. The designed protein sequence is ",
    "response#1": "MKILVTGGGTGGHIYPALAFVNYVKTKEPNTEFMYVGAQRGLENKIVPETGMPFRTLEIQGFQRKLSLHNLKTIQLFLKSIREAKKILKEFKPDVVIGTGGYVSGAVVYAASKLAIPTIIHEQNSVPGITNKFLSRYVDRILVSYPESLPFFKNKNFTGEVLRTGNPVRQEVLALPERPNAFSLADNQPIKVLVVGGSLGAAALNDVVPKAIALLPEGQRPVVKHQAGAKQIDTLRANYAAAGVAGDTVPFIDDMAAAYADADLVICRAGAMTVSEVAAAGVAALFVPFPHAVDDHQTTNATFLSKQGAALLVQQNELTAEGLAKTLAGLSRTQLKDMARAARGLAVLVVGGSQGARVMSDIVPGAIEKLGSPLWQRLVLTQQVRDEDMARVRAVYERLRINAELAPFFADLPSRLASSHIIVSRSGAGTVAELGAIGRPSILVPLPGAIDQDQFANAGVLADVGGAIRIVQSDFTPDRLAAELSALAADPARLAAMAAAARTVGRLDAAERAADIICEQVLEVSHA",
    "response#2": "MNILLAAGGTGGHLFPAEALGVELMKRDLRVRLVTDSRALRYSGLFSKDMTDVVPSETVRGRSPLALARTGLMLATGTVVALNLMRRLKPAAVIGFGGYPTLPPLIAARLKGIPTVIHDANAVMGRANRLLSRRVNAILTALPGAFPAAPTLTGMPLRAAIAALPRPQARAEARAALGLAPDRPVLVVGGSLGAAALNDVVPKAIALLPEGQRPVVKHQAGAKQIDTLRANYAAAGVAGDTVPFIDDMAAAYADADLVICRAGAMTVSEVAAAGVAALFVPFPHAVDDHQTTNATFLSKQGAALLVQQNELTAEGLAKTLAGLSRTQLKDMARAARGLAVLVVGGSQGARVMSDIVPGAIEKLGSPLWQRLVLTQQVRDEDMARVRAVYERLRINAELAPFFADLPSRLASSHIIVSRSGAGTVAELGAIGRPSILVPLPGAIDQDQFANAGVLADVGGAIRIVQSDFTPDRLAAELSALAADPARLAAMAAAARTVGRLDAAERTAERFAELVTELAR",
    "response#3": "MKLVLFAGGGTGGHLYPAIAMAAELQRLSPNVSVAFVGTKSGIEATEVPRLGYKLHLVSVRGLKRGFSPKLLIENLRILFDFARSLGITIQLLRSEAPDVVVGTGGFVSAPLLFAAQLLGKKTLIQEQNAFPGVTTRLLSLFASEVFVSFETARAHFPQAKQTVLTGNPRASEVLGLDTRSAQAQFGLSPDLPTLLIFGGSQGAQAINQAAFDSAEDFYQAGIQVLHVVGPKNADGPEDRTRGGVPYVVVPYVDEMELAYAAADIAMCRSGALTCAELTAVGLPAVFVPLAIGNGEQRLNAEPIVQAGGGLMVANSELSRDWIREHLIPLLTDTDRIVAMSEAAARLGRRDETALLNTLSQAGFRRVAEGEAGNRIAQLVLEMTGRAR"
  },
  {
    "reference": "MIPRDQPRKPDPVANDPATSSNASNAPAKTLLLLRFSGDLTTKADATRRRMTSRLVENLRMALKSSGRAGKVVKDRNRLFVALEKTDEPTLLDDDAMAERFARVFGIQSVSISHAIEYENVDHIVREGEAFGAPHVRDRKFAVRARRVGDRGETRISASKIERDLGSALLPLARGVDLKHPDTTVFVEVDPDQAYYFIANEPGAGGLPLGTEGRAVSLLSGGFDSPVASWRLLRRGVALDYVFCNLGGRQHELETLQIAKCLADRWQQGTRPRFHSVDFDPVSREIQAKVTTRYWQIILKRLMLRAAEAVAGRWEASAIVTGEAIGQVSSQTLQNLAVIETGAKLPVLRPLVGNNKEEIIAESRHVGTHDLSAKVGEYCAIVPSKPATHARLDDILEEEAKLDPSVLEQALEARTHYRLTDVDLSAWEAEDLAAREIGPNDTVIDLRPKAAYTTWHYPGALFLDFPNALRAYASFDSGQRYVLYCDFGLKSAHLADLMRREGLEARHVSGGLREVRRLAEAGGN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein have THUMP, Rhodanese domains with specific sequence motifs that are crucial for its activity.2. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor; Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate; This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway; The sulfur is donated as persulfide by IscS..3. The ATP binding site should be located in a region of the protein that is accessible to the ligand.4. The protein design should take into account the physiological conditions of the Cofactor biosynthesis, thiamine diphosphate biosynthesis pathway.5. The protein with the reaction [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 +   S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier   protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-   cysteinyl-[cysteine desulfurase], the Cysteine persulfide intermediate must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "IVKPHPEIFVKSESVRKRFTKILECNIRNIVKSRTESVAVYNRRDHIEVTAVSDQYYQEVVEILTHTPGIHHVLEVEQSEFTDLHDIYQQVLAHHRHNIENKTFVVRAKRRGKHDFTSIELERYVGGGLNQAVESAKVKLNKPDITIKVEIADDKLNLIIARHKGLGGFPLGTQEDVLSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQVASYLWNKYGSSAKVRFISVDFEPVVAEILEKVDDGQMGVVLKRMFMRAAGMIAERFGIQALVTGEALGQVSSQTLTNLRHIDGVTDRLILRPLINWDKEDIINLARDIGTEDFAKTMPEYCGVISRKPTVKAVKEKLEAEEDNFDFSILDDVPILGAQDPVLSLISGGFDSTVSTYLCIKRGLRAHYCFFNLGGRAHEIGVKEVAYYLWNKYGASHRVKFVTVPFEEVVREILEKVDNAYMGVTLKRMMLRAAEKVAESLEIPALVTGESVAQVSSQTLINLNAIDRAIDMLVLRPLATMDKGDIIDISRKIGTESFAASMPEYCGVISVNPTTKPKLIAALQRIPGIANILKVESHELEGIDDRFEDIADICIKTYGDQVSGKSFVVRVKRAGQHSFASTDLERYLGGAFLQNTKASRVELREPDVAIKLEIRDQTLYAIAEPKLIAALQRIPGIANILKVESHELEGIDDRFEDIADICIKTYGDQVSGKSFVVRVKRAGQHSFASTDLERYLGGAFLQNTKASRVELREPDVAIKLEIRDQTLYAIAE",
    "response#2": "VKPFAEITIKSRPVRRQFILQLGRNIRNVLKDLDPEIRTSGEWDNIDVVSSLTDPALQQQLVERMRHLPGIAHFYQVLDFDLPDSMQDIADRCAEHYAPLLKDKEFAVRCKRVGKHEFTSMDVEREVGSLLRQQCGAAGIRLNRPDVEVRLEIRHRRLLVEFNRQEGLGGYPLGSIEQALVLMSGGFDSTVAAYQLMRRGLLTHFCFFNLGGRAHELGVMEVAQFLWQKFGRSHRTLFISVPFEEVLGDILQNVDNSQMGVILKRQMLRAAEKVADNLHLDVLATGEAISQVSSQTLINLSVIDKATDKLVLRPLVAAHKQDIINTAEAIGTAEFAKHMPEYCGVISVNPTTKAKLGRIVHEEESLVIDIRSADQQEAKPLDEQQVGAPVQHIPFFKLARRFAELDSQKTYLLYCEQGVMSQLQALYLQEQGYSNVAVLRNIDEIVEELDQDLAVPVVDVVQPGQALIDIRHPDEEEANPLRVSGVDILKIPFYRLNNQFAQLDTGKQYFLYCQKGVMSQLHAANLKDAGHTNVGVYRL",
    "response#3": "IVKPHPEIFVKSESVRKRFTKILECNIRNIVKSRTESVAVYNRRDHIEVTAVSDQYYQEVVEILTHTPGIHHVLEVEQSEFTDLHDIYQQVLAHHRHNIENKTFVVRAKRRGKHDFTSIELERYVGGGLNQAVESAKVKLNKPDITIKVEIADDKLNLIIARHKGLGGFPLGTQEDVLSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQVASYLWNKYGSSAKVRFISVDFEPVVAEILEKVDDGQMGVVLKRMFMRAAGMIAERFGIQALVTGEALGQVSSQTLTNLRHIDGVTDRLILRPLINWDKEDIINLARDIGTEDFAKTMPEYCGVISRKPTVKAVKEKLEAEEDNFDFSILDDVPYVSENQVIVDVRAPNEQERKPLNMPGCEIILVPFYELEAAAVTWAEDKEYLLYCDKGVMSRLHAQNLQEKGFDHVKKIDIREIAEKAQEEVAEVETVASFSHNDVILDIRSNDEQEARPLEVEGVVVKSLPFYKLATQFGDLDQSKSWLLYCERGVMSRLQALYLHEQGFKNVKVYRK"
  },
  {
    "reference": "MKLSRCIEDLTVLEHLGPKDKEINSVCFDSRLAAPSCLFVAQKGSRSDGHDYIPEVVAKGAAVIVCENLPSEINPEVTYIRTGNSNLALGLISAAWFSHPSRKLKLVGVTGTNGKTTIASLLYNTLKALGYKVGLLSTVCVRVDEQELPATHTTPDAYAINSLLSQMLAAGCEYAFMEVSSHAVDQERIAGLHFAGGIYTNLTHDHLDYHKTVEAYLRAKKSFFDRLPAEAFALTNADDKNGKVMLQNCRAAHYTYSCRSLADFRARLLETHSDGMQLDFDGREVHVRFIGKFNVSNLLAVYGTAVLLGMDAAEVLRIISTLRPVSGRLEFLNSPTGYTAIVDYAHTPDALKNVLQTLAQIRKQGKIISVVGAGGNRDKTKRPEMALEAVRASDTLILTSDNPRFEEPQAILDDMYAGVPAEVRKRVLSIVDRREAIRTACRLAGKGDYVLVAGKGHEDYQEIKGVKYPFDDKKIITEIFKELL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate, ATP binding.2. The designed protein should be involved in cell wall organization, cell division by localizing to cytoplasm and exhibiting magnesium ion binding.3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.4. The designed protein must have at least one Mur ligase C-terminal domain.5. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.6. The protein's functional design necessitates the inclusion of a Meso-diaminopimelate recognition motif motif.7. The protein should have a low Km value for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "MKLSDLLKKTEYKILKGNMDIEEVKGLSTNSHACQPGDLFIGMQGTRVDGGEFWESAITSGAIAAIISPQAAAKCPSQGKACIADESGKIMTEAAAAFFNRYPSEMKLVGVTGTNGKTTTTLLIEYLLTYVGHKCGIIGTLYTRYKNQEYDSEHTTSTTPESLELQKLFIKMRDSGVEYCVMEVSSHALSLYRVAGLDFKAGVFTNLTQDHLDYHKTFENYYNAKFKLFNMTKNYCFNGDSDEFKERITQKGNSISFSVEKEEADICVKNVQLSSRGSSFSMEYKGRSFDISCSLIGKFNVYNSLAAITAALLEGIPLEDIKKALASVKAVSGRMERIDEGQDFLAVVDYAHKPAAVAAVLDTLRGQVTGRLGVVVGAGGDRDASKRPIMGEEAARRADLVIITDDNPRSEVPAVIRAAVLEGARSAGTDAEIREIGDRAAAIDALIDWARPGDGVVVAGKGHETYQIIKGRKIPFDDREVALEIIK",
    "response#2": "MKLKELISNVEIKLVKGDIEKEEISAIEFDSRKVQKGHLFVAIPGTQVDGHQYIEKAIDLGASAILCERLPETLKEQVTYIQVENSAKAMGLAAANFYDKPSKKLKLVGVTGTNGKTTTTLLIEYLLTYVQPPKTTALFGTLYTRWPGFEQTASYTTPFAVELQEQLRAAVTAGCQSAVMEVSSHALAQHRVMGCPFEVAVFTNLTQDHLDFHKDLEDYFAAKALLFSPGYLQGRAVINADDPYGRRLIEKLDKEQVWSYSVDNSAADLWTTDLNYDPNGVSGKLHTITGEAPFNLPLVGQYNIANMLAAVGAVYALGFDFKDILTTIENIKTVEGRLEVVPTGRDFTILIDYAVTPDALDSMIRTVRQTARGRVGVLFGCGGDRDRTKRPKMGRVVASLADYVIITSDNPRTEEPEAIIRDILEGLADITTPYVVIPDRREAIRWAIENAQSGDTIVLAGKGHETYQILKDKTIEFDDRKVALEALK",
    "response#3": "MRLSDLLKNIPVLEAKGNPSLDVSGMTLDSRHVKQGDVFVAIKGHSVDGRQFMAKAIENGAAAVLTHNDDNTFSLTEQNGVAVIALPSLNTLLSAIAKRFYGNPSEKLKLVGVTGTNGKTTVTHLIEEVFHDLGEGTGLIGTLYRKVNDQVYPTTNTTPDVLTNQKTLAEMRDANVKTVSMEVSSIALVQGRVWGINYDICVFTNFTEDHLAYHKTMSQYKLAKSLLFAQMGNTFSGKTAVMNIDDPVGREFIDYTTSNILTYGIKHQADIYADHIKITIHGTEFDLHVFDEVKHVKTHLIGEFNVYNSLAAVGAIAMELGVSLEKVLEVLPTFPGVPGRFQLVPSNTGVSVIVDYAHTPDGLLNVLETLNDFAEQDTYCIVGCGGDRDAQKRPQMAKIAVDNSTHAILTEDNPRTEDPHKIMDDMLAGIDSDQATVIFNRREAIQYAVNRAKPGDAIIIAGKGHETYQIIGKEKYNFDDRLVASAALRCLLA"
  },
  {
    "reference": "MARIFETNKLVIASHNLGKVREIGELLAPFGTETLSAGELGLPEPIEDGITFIANAEIKALAAATASGLPALADDSGLEVEALGGEPGIYSARWAGPSKDFTVAMAAINGKLSGITNMAANFTCALTLAWPDGHMESFEGKVFGTLTWPLRGNRGFGYDPMFIPTGHAVTFAEMEPSEKHAMSHRAHAFKQLIDACFKNS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..2. A protein with dITP diphosphatase activity, ribonucleoside triphosphate phosphatase activity, XTP diphosphatase activity, ITP diphosphatase activity, metal ion binding is needed to enhance nucleotide metabolic process, purine nucleoside triphosphate catabolic process in a specific context.3. The substrate, Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.4. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in dITP + H2O = dIMP + diphosphate + H(+) are properly positioned for optimal catalysis.5. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "MIYVSNKAKLQNISLTFRNHSKNSIMPLAIVTGTDTGVGKTVVTAALAYYYQNMGINVGIIKPVQTGVIRGDFEDASDLKIIAGINIPLDYINPLAFEYPLAPHIAAKKENIEIDISKIKKNFLKLQKKHDFTLIEGAGGLYVPLTKNKTMLDLMKELGLPVILVVNIKLGTINHTILTLSYLKNNKINIKAIIFNKYKNNINKVIKTTNIKMIKKLCNIPILSTIPYKKNIIKKNIFKKYFILNYFKNKI",
    "response#2": "MILLATRNKGKAEEFKALLEPFAVRVIGLEDLPGVGEVPETEETFEGNALLKARAVAAHTGLPAVADDSGLCVDALDGAPGIYSARYAGEPCSDEANNRKLLAELKGTAPPQRGAAFVSVVAVATPGGEELVVEGTVRGRILAAPRGDGGFGYDPLFYVPEFGKTFAELEKEEKNRISHRGKALERLKEFLRGEA",
    "response#3": "MPTTGAPTAPVRVVLATHNAHKVAELRRLLGAGLDGVEIVSAGDLGVPEAPEPHRTFVDNALLKARAAAAASGLPALADDSGIAVDVLGGAPGIFSARWSGVHGNDRANLDLLLAQLSDVPEARRTARFVCAAALVLPGGREEVVRGEWPGTLLTAPAGEGGFGYDPVFVPEGEERTTAELTAAEKNAISHRGRAFRALAPRVAALAGG"
  },
  {
    "reference": "MSQITAIILAAGLGTRMKSALPKVMHPVAHRPMIRHLITTLETATQGDDLRIVAVIGPDMPVLEAAVAPHPFVIQHDRRGTGHAVQMALPELPDSGTVLILMGDAPLLRPETVRRLIETRQTKGAALLVGAFRPVNPTGFGRIIQAADGSITRIVEEKDLAPDQKAIPLCNGGIYAVDAARLPGLLARLTPNNAQGEFYLTDIVEHAVSDGALVLPLDIAADEIHGVNSRADLAFVEGIAQARYRAAAMAGGATLLDPASVYFAADTKIGQDVTIGPNVVFGPSVTVADRVTIHAFSHIEGATVASGAIIGPFARLRPGAQIGDGAHVGNFVEVKNAVLGAGAKANHLTYLGDADIGAGANIGAGTITCNYDGINKHRTIVGAGAFIGSNSALVAPVVIGDGALIGAGSTITRDVPADALAVVRGELVQKEGAAERFRETRRAAKAKKKD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a high specificity for acetyl-CoA, UDP-N-acetyl-alpha-D-glucosamine, Mg(2+) binding, with minimal off-target effects.2. A protein that can perform glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity, magnesium ion binding in cytoplasm to support lipid A biosynthetic process, cell wall organization, peptidoglycan biosynthetic process, UDP-N-acetylglucosamine biosynthetic process, cell morphogenesis.3. The presence of Mg(2+) is necessary for the protein to function in the desired environment.4. The protein contains novel MobA-like NTP transferase domains that confer a unique function or activity.5. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.6. We require a protein that can specifically bind to the metabolites produced in the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway. The designed protein sequence is ",
    "response#1": "MISAILAAGRGTRMKSDYPKVLHSLGGRSLVERALNSCELINPSRFLVIVGYQADLVKQALSHLDGIEFVEQSEQLGTGHAIQTLLPILGDFNEDLLVLNGDVPLLRPETIKELLTTHKQHQNAATLLTGFIADDPTGYGRIVRQGDRVVAIREHKDASEAERAISEINSGVYAFDVAALRDGLSRLSNDNAQGEMYLTDALELARNDGGTVAAHRVDDPMLVEGVNDRVQLARLGAEYNRRICERHMRAGVTLADPARIDVRGRLVCGRDVEIDVGCIFEGEVTLGDNVRVGAYSVIRNATVATGTDIAPYSHIDSSAVGANCHIGPYARLRPGTKLADGAHIGNFVEIKNSEIGAGSKANHLSYIGDSTVGSRVNIGAGTITCNYDGANKFRTVIGDDAFIGSDTQLVAPVTVGRGATIGAGSTITKDTPDNELTLSRSKQRNIAGWVARKRPGSAAARAAERARAQDAQND",
    "response#2": "MKTVVVLAAGEGARMQSSTPKVLHELAGRSLLGHVVHAAATLRPRNLAVVVGHESSRVSEHLHQVIAPELDPSVVATLSTPFQKQQAGTGNAVAVACQALAEERLDAGEHMVVLNADTPLLRPQTLRQLLQQHVDSGAAATVATVLTLSADDPTGYGRIVRDAAGAVSAIVEEKDATDAQRRVTEVNSGIYAFDAAVLRDALGRLSTDNSQGEQYLTDVLAILREDGHRVGATRTSDAWLTEGVNDRVQLATLGAEYNRRVCERHMRAGVTLADPARIDVRGRLVCGRDVEIDVGCIFEGEVTLGDNVRVGAYSVIRNATVATGTDIAPYSHIDSSAVGANCHIGPYARLRPGTKLADGAHIGNFVEIKNSEIGAGSKANHLSYIGDSTVGSRVNIGAGTITCNYDGANKFRTVIGDDAFIGSDTQLVAPVTVGRGATIGAGSTITKDTPDNELTLSRSKQRNIEGWVTRTRPGTAAADAARRAGEDPALAAEQTRARVAELSARSGTRPD",
    "response#3": "VIVLAAGEGTRMKSRTSKVLHELCGRSMLGHVLAAARELDPGRLIVVVGHRREQVAAHLAEHVPAAEPVVQERQGGTGHAVRTVLDQVGALTGTVVVTNGDHPLLRGETLAELVRTHETEGNAVTVLTTVDVADPTGLGRIVRDSDGRVVAIVEEKDATLEQVAIREINTGIMAAPTARLTEWLGRVTTDNAQGEYYLTDLIALAHRDGVDVTPVQVAQAWEVMGVNSKVQLAELERRHQLDNARALMDAGVTLADPARIDVRGRLVCGRDVEIDVGCIFEGEVTLGDNVRVGAYSVIRNATVATGTDIAPYSHIDSSAVGANCHIGPYARLRPGTKLADGAHIGNFVEIKNSEIGAGSKANHLSYIGDSTVGSRVNIGAGTITCNYDGANKFRTVIGDDAFIGSDTQLVAPVTVGRGATIGAGSTITKDTPDNELTLSRSKQRNIENWVRPVKK"
  },
  {
    "reference": "MIRVLVTGAAGKMGREVVKTVTAAEGMSVVAAVDPGCDGLSVDDGSGNQIVCTGDLAAAITMHAPNVMVDFTHPDAVEGNLRIALQAGVDCVVGTTGLSEGQLRALASDAPEGTCLFVAPNFAIGAVLMMRFAQEAARFMPHVEIIELHHDKKADAPSGTALRTASLIAAGRCDVPEVPGKETEITPGARGAFVDDIAIHSVRLPGLVAHQEILFGGQGQTLSLRHDSIDRTSFMPGVVLAVSGVGGLSGLVIGLETLMGE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein with the reaction (S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-   4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH, the Proton donor/acceptor, Proton donor must be optimized to ensure efficient catalysis.2. The designed protein should have a binding site that is specific to the target NAD(+) ligand.3. The designed protein have Dihydrodipicolinate reductase C-terminal, Dihydrodipicolinate reductase N-terminal domains with specific sequence motifs that are crucial for its activity.4. The protein should have Helical domains to ensure proper insertion into the membrane.5. A protein that contributes to lysine biosynthetic process via diaminopimelate, diaminopimelate biosynthetic process by functioning in cytoplasm, membrane with NADP binding, oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor, 4-hydroxy-tetrahydrodipicolinate reductase, NAD binding.6. The protein design should consider the endogenous proteins and enzymes present in the Amino-acid biosynthesis, (S)-tetrahydrodipicolinate from L-aspartate: step 4/4 pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MRVGVLGARGKVGTTMVEAVEAADDLTFAAGVDADDPLSRLVDAQTEVVIDFTHPEVVMDNLRFLVENGIHAVVGTTGFTDERLAQVRQWLDAKPGAAVLIAPNFAIGAVLMMRFAEMASPFFDTAEIIEIHHDAKADSPSGTAVATAQRMAAASSQWAKDPTTHEAIPGGRGAKGPGNIPIHAVRMRGVVANQEVILGTQSQTLTIRHDTIDRASFMPGVVLACKKIAEHKGLTLGLDSLF",
    "response#2": "ISIMVNGCSGKMGKAVIKAADSAGVNIVPISFGSAGEDGQRVEVCGKEITVHGPTEREKVLSSVFEKHPELIVVDYTIPSAVNDNAELYSKVGVPFVMGTTGGDRNKLYETVEEAKIYAVISPQFSVGAVLSMHFAQQAARYFESVEVIELHHPHKADAPSGTAARTARLIAEARKDMPPNPDATSTGLEGARGADVDGVPVHSVRLAGLVAHQEVLFGTQGETLTIRHDSLDRTSFVPGVLLAVRRVAERPGLTVGIL",
    "response#3": "MRVGVLGARGKVGTTMVEAVEAADDLTFAAGVDADDPLSRLVDAQTEVVIDFTHPEVVMDNLRFLVENGIHAVVGTTGFTDERLAQVRQWLDAKPGAAVLIAPNFAIGAVLMMVLAAHAAPYFRRAEIIERHHENKRDRPSGTSLRTAALMNTARSQAWSVEPDSRELLEGVGGGDAGGVRVHSVRMPGSVAHQEVILGSPGETLTIRHDSIDRTSFMPGVILALKKVRSLSGLTIGLEHLL"
  },
  {
    "reference": "MDGVDVPAGQAGKELLRFLTCGSVDDGKSTLIGRLLHDSKQIFQDQLSALARDSARYGTTGGHVDLALLVDGLEAEREQGITIDVAYRFFSTSKRSFIVADTPGHEQYTRNMATGASKADLAVLLIDARKGVLVQTRRHSLICSLLGLRHVVLAVNKIDLVSYDSDIFERIVSQYRAFASELGFSSIAAIPISARNGDNVMRRSDQTSWYRGPTLLDYLETVDVTQEMTGKPFRFPVQWVNRPGPDFRGYAGTVASGTIGVGDPITAAGSGQTSRVKELITYDGPQSSAQAGDAITLTLTSEIDISRGDLLVSPASDTEISDQFAAHIIWMSDQALMPGRSYLARIGTRTTPITVTGIKYRIDVNTREHLATHTLGLNDIGFCNLSTGTPVAFDPYEQNRKTGSFIMIDRLTNHTLGAGMIAFGLRRGTNIHWQATLIGKAERAAHKHQRPAIIWLTGLSGAGKSTIANMVERQLHAAGHHTMMLDGDNVRHGLNRDLGFTEPDRVENIRRAGEVAKLMVDAGLIVLCSFISPYRAEREAVRRLVADGEFVEVFVDTPIDECAKRDPKGLYAKAKAGEIKNFTGFDAPYEAPENPEIHLQTIGQQPEQLAQRLVRTLTELGIVG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein with the reaction adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate   + ADP + H(+), the Phosphoserine intermediate must be optimized to ensure efficient catalysis.2. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.3. A protein that is capable of catalyzing reactions within the Sulfur metabolism, sulfite from sulfate: step 2/3, hydrogen sulfide biosynthesis pathway with high efficiency and specificity.4. The designed protein should be optimized for adenylylsulfate kinase activity, GTPase activity, ATP binding, GTP binding activity in order to improve sulfate assimilation, hydrogen sulfide biosynthetic process.5. For general function, the protein need meet that APS synthesis involves the formation of a high-energy phosphoric- sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD. The designed protein sequence is ",
    "response#1": "SLMRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAILESDSKKVGTQGEEIDFALLVDGLAAEREQGITIDVAYRFFTTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILVDARKGVLVQTRRHSYLVHLLGIKHVVLAVNKMDLVDYDQATFDAIVDDYTAFAKEVGIEEFTAIPISGFKGDNIELVRFSTAGSVDDGKSTLIGRLLHDSKCIYEDQLSSIKKASKNRDIPIDFALLTDGLKAEREQGITIDVAYRYFSTSKRKYIIADTPGHEQYTRNMATGASTADMAVILVDAVNGLLPQSKRHAFISTLLQIPHIIIAVNKMDLVDYRQAVFDDIVNEFTLFAEKLQIHDLRFIPISALHGDFVVDTKSDKMPWYQGPLRFPVQLVSRPDRTFRGYSGQLSSGILRKGTEIMALPSMQKSRVKSIVTFDGELEEARPGQAITITLEDERDISRGDMLVHPSNRPEVADQFEATIVWMDDEAMLPGRAYLLKLATQTVSATIQAPKYEINVNTMEHLAAKTLELNVIGVAEITTDKPLVFEAYAENRALGGFILIDKINNRTVGAGMLHFALRRSHNIHLQRVDVDKAARAASKGQQPLCLWFTGLSGSGKSTIANLVEKRLHALGRHTYLLDGDNVRHGLNKDLGFTDEDRVENIRRVAEVAKLMTDAGLIVLVSFISPFRAERRMAREMFAPGEFVECFVDTPLDVAEARDVKGLYAKARAGKIANFTGIDSPYETPENPEIRVDTTLLSAEQAADYIIE",
    "response#2": "RFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAILESDSKKVGTQGEEIDFALLVDGLAAEREQGITIDVAYRFFTTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILVDARKGVLVQTRRHSYLVHLLGIKHVVLAVNKMDLVDYDQATFDAIVDDYTAFAKEVGIEEFTAIPISGFKGDNITAKADGAPSSNTPWYSGPALINHLENVELKSLLRFITCGSVDDGKSTLIGRLLYDTKLIFDDQLAALEKDSRKYGTTGPAIDLALLVDGLEAEREQGITIDVAYRYFATAKRTFIVADTPGHEQYTRNMATGASTAGLAVILVDARKGVLTQTKRHSFICSLLGIKNIVVAINKIDLVDYSHVVFAEIEEAYRDFAEQLDFDSITPIPMSALTGANVTTPSEDLPWYSGPTLLEHLEEVDVDTDKSLMRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAILESDSKKVGTQGEEIDFALLVDGLAAEREQGITIDVAYRFFTTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILVDARKGVLVQTRRHSYLVHLLGIKHVVLAVNKMDLVDYDQATFDAIVDDYTAFAKEVGIEEFTAIPISGFKGDNITAKADGAPSSNTPWYSGPALINHLENVELANFRFPVQFVSRPHLNFRGFQGQVASGRVAVGDTVAVAKSGVTTRIKRIVTMAPDGTDLDLDQARDGQSVTFVLADEVDCSRGDMLVAPGNVPAEVSDQFEATIVWMDEEALLPGRGYWMKLASQTVSATVAEPKYEININNVGGPGDHLAAKTLELNSIGVAEVTTDRPVVFEPYADNRALGGFILIDKITNRTVGAG",
    "response#3": "TMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALENDSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADCPGHEQYTRNMVTGASTADLAVILIDARKGVLVQTRRHSYLCHLLGIRNIVLAVNKMDLIDYDQARYDAIVAEYETFAKGIGIESFTAMPISGFKGDNIESLFNYIEKVSLLRFITCGSVDDGKSTLIGRLLHDSKLLLDDQVSALESDSRRYGTQNGEIDYALLVDGLAAEREQGITIDVAYRFFDTARRKFIVADCPGHEQYTRNMATGASTADLAVVLVDARKGLLTQTRRHSYIVSLLGIRHVILAVNKMDLIDYDQVAFERIAADYRELAAKLGIDHVHCLPLSALKGDNVSSKSSKGMRMPVQWVNRPDHTFRGFSGQLVSGTVAPGDAVRVLPSGQTSTVERIVTMDGDLDPAVAGQSVTLTLADEIDVSRGDVIAHPQVSSQFAAEVLWMHTDEMLPGRSYLLKAGGQTTPVSVTTLKHKLDVNTFDKEPARKLELNEIGVCNLSTSRPIAFDAYKDIRETGSFILIDRYTNLTVGAGMV"
  },
  {
    "reference": "MSAVSQPQAAHAPLEKPASTAILCNTCGNVCKGEVLRVQNKHFHIRCFVCKACGCDLAEGGFFVRQGEHICTRDYQRLYGTRCFSCDCFIEGEVVSALGKTYHPDCFVCAVCRLPFPPGDRVTFNGKDCMCQKCSPPTLVGNSAHVAQGLRSCGGCGLEIKNGQALVALDKHWHLGCFKCKTCGKLLNAEYISKDGLPYCEADYHTKFGIRCDGCEKYITGRVLEAGEKHYHPSCALCVRCGQMFSEGEEMYLQGSSIWHPACRQAARTEDKSKETRTSSESIVSVPASSTSGSPSRVIYAKLGDEILDYRDLAALPKNKAIYNIDRPDMISYSPYISHSAVGDRQSYGEGDQDDRSYKQCRTSSPSSAGSVSLGHYTPTSRSPQHYSRPGSESGRSTPSLSVHSDSRPPSSTYQQAPRHFHVPDTGVKDNIYRKPPIYKQHAARRLDVEDSSFDQDSRKKTTWLLLKGDADTRTNSPDLDSQSLSLSSGADQEPLQRMPGDSLYSRFPYSKPDTLPGPRKDGLDLRNANLAPCGADPDASWGTREYKIYPYDSLIVTNRIRVKLPKDVDRTRLERHLSPEEFQEVFGMSIEEFDRLALWKRNDLKKKALLF",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that can perform actin filament binding, metal ion binding in myofibril to support cytoskeleton organization.2. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding.3. The designed protein must have at least one LIM zinc-binding, HP domain. The designed protein sequence is ",
    "response#1": "RAASANLPSLFGGEFQAIAYTSIIDDYAHLALVRGEIRPEDEVLVRVHSECLTGDVFGSRRCDCGDQLRAALRQIDREGKGVLLYMRNHEGRGIGLVNKIKAYALQEQGYDTVEANLALGFKDDLRDYGIGAQILVDLGIRKMRLMTNNPKKIVGLEGYGITVTERVG",
    "response#2": "RLRAHSIPGLTARLNSFRVKISFKHSIMFGYNSVRPFDIRLLHESTSLDSAKTHRVSWGKKSFSRGKYYWEVDLKDYRRWTVGVCKDPWLRGRSYVVTPTDIFLLECLRNKDHYILITRIGREHYIEKPVGQVGVFLDCEGGYVSFVDVAKSSLILSYSPGTFHCAVRPFFFAAYR",
    "response#3": "RAASANLPSLFGGEFQAIAYTSIIDDYAHLALVRGEIRPEDEVLVRVHSECLTGDVFGSRRCDCGDQLRAALRQIDREGKGVLLYMRNHEGRGIGLVNKIKAYALQEQGYDTVEANLALGFKDDLRDYGIGAQILVDLGIRKMRLMTNNPKKIVGLEGYGITVTERVR"
  },
  {
    "reference": "MDIEMAKEPVNPTDTPDIEVTPGLCQPLRRRFRRFVTAKQLYECLRPVFHVTYIHGLTSFYISCDTKTGKKAIKKTIFGYINGIMHIAMFVFAYSLTIYNNCESVASYFFRSRITYFGDLMQIVSGFIGVTVIYLTAFVPNHRLERCLQKFHTMDVQLQTVGVKIMYSKVLRFSYMVLISMFLVNVLFTGGTFSVLYSSEVAPTMALHFTFLIQHTVIAIAIALFSCFTYLVEMRLVMVNKVLKNLAHQWDTRSLKAVNQKQRSLQCLDSFSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLGKSKRESKFKTVEFVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLLNKTKSAEVKEKLQQFSMQLMHLKINFTAAGLFNIDRTLYFTISGALTTYLIILLQFTSNSPNNGYGNGSSCCETFNNMTNHTL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should contain a Helical; Name=2, Helical; Name=3, Helical; Name=6, Helical; Name=4, Helical; Name=5, Helical; Name=1 membrane-spanning region.2. The Extracellular, Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.3. For general function, the protein need meet that Probable gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates ; Atypical expression suggests also nongustatory roles in the nervous system and tissues involved in proprioception, hygroreception, and other sensory modalities4. The designed protein should have taste receptor activity within neuronal cell body, axon, membrane, plasma membrane to regulate sensory perception of taste, negative phototaxis, phototransduction, feeding behavior. The designed protein sequence is ",
    "response#1": "MQAMRFAVEEINNDSSLLPGVLLGYEIVDVCYISNNVQPVLYFLAHEDNLLPIQEDYSNYISRVVAVIGPDNSESVMTVANFLSLFLLPQITYSAISDELRDKVRFPALLRTTPSADHHIEAMVQLMLHFRWNWIIVLVSSDTYGRDNGQLLGERVARRDICIAFQETLPTLQPNQNMTSEERQRLVTIVDKLQQSTARVVVVFSPDLTLYHFFNEVLRQNFTGAVWIASESWAIDPVLHNLTELRHLGTFLGITIQSVPIPGFSEFREWGPQAGPPPLSRTSQSYTCNQECDNCLNATLSFNTILRLSGERVVYSVYSAVYAVAHALHSLLGCDKSTCTKRVVYPWQLLEEIWKVNFTLLDHQIFFDPQGDVALHLEIVQWQWR",
    "response#2": "GIITVVNAVSWVQKRAVSITTRILLLLSVSRIGLQSIILIEMTSSIFNFSSYNSVLYRVSRVSFVFLNYCSLWFAALLSFFHFVKIANFSYPLFFKLKWRISELMPWLLWLSVFISFSSSMFFCNHKYTVYNNISLSSNICNFTMELYVAEANVVNVAFLFSFGILPPLTMFIATATLLIFSLRRHTLHMRNGDADSRNPRVEAHKQAIKETSCFLFLYIL",
    "response#3": "GIITVVNAVSWVQKRAVSITTRILLLLSVSRIGLQSIILIEMTSSIFNFSSYNSVLYRVSRVSFVFLNYCSLWFAALLSFFHFVKIANFSYPLFFKLKWRISELMPWLLWLSVFISFSSSMFFCNHKYTVYNNISLSSNICNFTMELYVAEANVVNVAFLFSFGILPPLTMFIATATLLIFSLRRHTLHMRNGDADSRNPRVEAHKQAIKETSCFLFLYILS"
  },
  {
    "reference": "MKTFSAKPHEVKRDWFVVDATDKVLGRLAAEIARRLRGKHKPEYTPHVDTGDYIVVVNADKLRVTGNKALDKKYYRHSGYPGGIYERTFTELQNEFPERVLEKAVKGMLPKGPLGYAMIKKLKVYAGAEHPHTAQQPKALEI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: It is important during the early stages of 50S assembly.; This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in ribosome, ribonucleoprotein complex that is critical for translation. The designed protein sequence is ",
    "response#1": "MENQTK",
    "response#2": "MRVPAQ",
    "response#3": "MVISL"
  },
  {
    "reference": "MSAAIRPLIAGNWKMNGLRASLSELKLMLEGAGKLSATADLLICPPATLVAAFAQKLAADTGSRSNSFAIGAQDCHPRASGAHTGDISAEMLADAGASAVIVGHSERRADHGESDALVREKAEAAWRAGLVAIICVGETQGQRDAGQTLDVVGTQLAGSLPDGATAANTVVAYEPVWAIGTGRTPTVADVEQVHGFIRKSLTQRMSGEGSKVRILYGGSVKPSNAQELMAVADVNGALVGGASLKAGDFLAIAAGCP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. We need a protein that can perform D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate with high efficiency.2. A protein that is capable of catalyzing reactions within the Carbohydrate degradation pathway with high efficiency and specificity.3. The protein must exhibit the following characteristics: Involved in the gluconeogenesis; Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D- glyceraldehyde-3-phosphate (G3P)..4. The designed protein must be able to perform triose-phosphate isomerase activity under specific conditions. The designed protein sequence is ",
    "response#1": "MLTITAVLFDMDGTLIDSEPLWLEAERVFAKRYGIELTREDALKLVHGRSLADTAALFREHDIDIDAEEIIAELVADVNALYQSQIPWRPGARELLDTLAAEGVPCALVTMSYRRMVDAAVQALPGALFAEVIAGDDVQHGKPDPEPYLLAAQKLDVQPANCLAFEDSVSGAAAAQAAGMRVIRVPEPDELPESLRADFIVESLTELTLEQLPQLLAAHT",
    "response#2": "MQQNNQFVPLN",
    "response#3": "MLLLDVDGVLNCVPGYHPATASLR"
  },
  {
    "reference": "MGGKWSKRSISEWTTVRERMRQAEPAADGVGAASRDLEKHGAITSSNTAATNADCAWLETQEEGEVGFPVRPQVPLRPMTYKGALDLSHFLREKGGLEGLIYSQKRQDILDLWVHHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPDKVEEANEGENNSLLHPTSLHGMDDPEKEVLIWKFDSRLAFHHVAREMHPEYYKDC",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be optimized for stability and solubility when expressed in host cell Golgi membrane where it will be involved in suppression by virus of host autophagy.2. The protein must exhibit the following characteristics: Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD..3. The designed protein should contain at least one Dileucine internalization motif; necessary for CD4 internalization motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "GSPGGFR",
    "response#2": "GNTSNN",
    "response#3": "GPPAAPPKA"
  },
  {
    "reference": "MDFIDLPQCLGAGGVVRLPGSKSISNRVLLLAALAEGETEIRDLLVSDDTERMLDALRTLGVNIQPLPGAAGTDEAYTALNVRVGGVGGAFSVREAGLFLGNAGTAFRPLTAVLALMGGRYRLTGTPRMHERPIGDLVDALRQAGADIRYLATEGYPPLAIGPRAVPDGGESARKESAGRIRVRGDVSSQFLSALLMALPLIGGGTVEVVGELISRPYINITLATMARFGVDVCAEGVSAFIVPAGSYRSPGVVHVEGDASAASYFLALGALAGGPVRVEGVGRQSVQGDVRFVEALTAMGARVSLGENWIEAAAPVSGRLAAIDLDCNAIPDAAMTLAPLAVFADGVSTLRNIGSWRVKETDRLAAMAVELAKLGACVEEGADFLRITPPTDGVRPAMIDTYDDHRMAMCFSLASVGAPVRINDPGCVAKTFPEYFQHFARLTRVVPVIAIDGPSASGKGTVAARVAATLGWNYLDSGALYRLTALAAQRAGVAWDDEPAVAAIAAGLDVVFAEGSIRLAGEEVEDAIRQEEISSGASQVAVLPSVRAALLFRQRAFCRAPGLVADGRDMASVVFTDAALKVFLTASVEARAERRYKQLMAKGIAARLQPLLLEMQQRDDRDRGRSVAPLQQDANASLLDTTDIPIDVAVARVLDWARAVVPDIAGAAV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate..2. The presence of Cytidylate kinase, Enolpyruvate transferase domains is critical for the interaction of the designed protein with its intended targets.3. The phosphoenolpyruvate, ATP binding site should be stable and able to withstand changes in environmental conditions.4. The protein should have ATP binding, 3-phosphoshikimate 1-carboxyvinyltransferase activity, cytidylate kinase activity and be active in cytoplasm.5. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.6. The designed protein should interact with enzymes and substrates within the chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7, chorismate biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MVAIDGPSASGKGTVGARVAAALGWHHLDSGSLYRLVALSAERGGIDLDDEVALAAIAGDLPARFEGERVWLGTDDVTDAIRSEQCSAGASKVAVLPAVRAALLGRQRDYRQGPGLVAEGRDIGSVIFPDASVKIFLTASVEARAERRYKQLIGKGLAANMESLMQDLRERDARDAARTVAPLQKLPDAALLDTTDRDVEQAVTFVLDLVRPALSAR",
    "response#2": "MPKRLFLGNAGTAMRPLTAALAVLGGDYELSGVARMHERPIGDLVDALRQLGCDIEYLGNEGFPPLRIGRPQLKLDAPIQVRGDVSSQFLTALLMALPLVAARDIRIDVVGELISKPYIEITLNLLQRFGIAVRREGWQRFTIPAGSRYQSPGEVHVEADASSASYFMALGAIAAGDGRTVRVAGVGEDSIQGDIRFVDAVRMMGAVVRGGPNWVEVARGAWPLRAIDLDCNHIPDAAMTLAVLALYADGPSTLRNIASWRVKETDRLAAMAVELRKLGATVHEGADFITIAPPRGWRAASIHTYDDHRVAMCFALAAFNHDGVAVRIEDPACVAKTFPDYFEALFAAITADPAEVVAIDGPSASGKGTVAARVAESLGYHYLDSGSLYRLVALATIRGGVTDDAAIAAVAEALPAEFVGGKILLSGADVTDAIRSEECAAMASKVAAIPAVRDALLERQRAYRQAPGLVAEGRDMGSVIFPDASSKFFLTASVEARAQRRYKQLMEKGLPANIDSLSQELSERDARDAARSVAPLKKLPDAVLVETSTLSVKDSVEKVVELVVERSA",
    "response#3": "AAGTVRLPGSKSISNRVLLLAALADGQTRVHDLLQSDDTERMLDALRTLGVPWQHDAGADCFVVDGVAGRFPVADADLFLGNAGTAFRPLTAALALSGGDYRLSGVARMHERPIGDLVDGLSQLGAAVTYTGNPGYPPLTIGKGTICAGGTVSVRGDVSSQFLTALLMAAPLTGVAVHIEVVGELISKPYIEITLNLMARFGVQVEREGWQRFTVPGGARYRSPGDIHVEGDASSASYFLAAGAMGGGPVRVEGVGRNSIQGDVRFAEALEQLGVQITMGEHWIEASAPASGRLKAFDMDLNHIPDAAMTLAVMAVRCDGPCHLRNIASWRVKETDRIAAMATELRKIGVEVEEFDDALRVTPPQTLRHAAIDTYDDHRMAMCFSLVTLAGTGVTINDPACVNKTFPGYFG"
  },
  {
    "reference": "MLSQYDEQLAAGDNNGFNKQGNATLYSFDFVDADDFLDSISGALPNNGHNNVNPNTNDISFEDMNIMNPNIYSPVSAASGDDFTQSSGQPMISEGSNYTGQNFTDYLSDNSLEGYDKNTSRPLHEVDIGFSNKRSNSTSTGSLSHNEEITPISHYSVDSIVTSPEPPINKQGDFPPIKRTTTVSSTNSITNTTKKPAKVTKPKSKDKNSHNMIEKKYRTNINTKILALRDAVPALRIAAGCDDVSIADLEGLTPASKLNKASVLTKATEYIKHLESKNFILKQQNIELHRLIQHANMNPKSLPPPPQQMQAPPQPGFGFYPPQNQSFNVTPASQYPSPQQQVSPTQQQTVHHPPQPNRYLLGGMAAVMGTSLFGGSGENDFRSLSALPFSYLFPNAILNPSPLTIQLWTLTKVLLVVGSLASIFIPMYKQAQLKKEDKPNTIPETSLLDWILISIGFKTPAKLSVSKRDAIISNLQGGNDWSQLVSDYFYLAGCEINFENCFLSLVLGTIIRHRFPVVATILNHYLSMKEALLLNLDYKGFSKSLIRLNQLISKVDGVSIFESTNLTTRLTNVFTNNRINANIVDGQNHVKYIEFYQRNINDYYAIVFNWRLLEFIHELNVTYLEQLNDDQSQVLTDLKIIEAFFGEQDNKLFGYYQLFTSILNANYAPYLFESLKDKVESSLEKFRIAYEGIDLTDHEIHNTSSEDEYEQESPVVYKYEPTLKSQKSLISSLNLVNEEEFIILTCSLTIYYYKNKEYDRALKLLNYL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be optimized for stability and expression in nucleus where it will participate in positive regulation of transcription by RNA polymerase II.2. The protein must exhibit the following characteristics: Regulates also gene expression during intestinal colonization but is not involved in host cell adhesion.; Binds directly to the two SRE-1- like elements upstream of TEC1 and thus regulates positively expression of this important hyphal growth regulator. The designed protein sequence is ",
    "response#1": "TEMVQDLRDDYKPAMNHDQKLLSFKKVGDKHTIVDHKGRIHEIEIEAQLHGMFFLSEFPSPGDGNVLNAELTCYRRNLFQISGNICFPQIPLSVMLETGETSQIKNMEVTISAIESVDGHPVRLIVIPWKTPPPNSPEVNQAPDQEPPSLPLIPWSEEEEDNGGDHYAIYPIGWRRLQFRIATANNGRRKELQQHFVLHLKLHGTLANGTKLVLSELTTAPIVVRGRSPRNFQARL",
    "response#2": "ELELYLEEALTQRTPDFDVLKWWQDNTLKYPTLSRMARDVLAIPMSTVGVGSSVFLPDNGSRSLDDYRSSLRPELVEALLCAKDWR",
    "response#3": "TEMVQDLRDDYKPAMNHDQKLLSFKKVGDKHTIVDHKGRIHEIEIEAQLHGMFFLSEFPSPGDGNVLNAELTCYRRNLFQISGNICFPQIPLSVMLETGETSQIKNMEVTISAIESVDGHPVRLIVIPWKTPPPNSPEVNQAPDQEPPSLPLIPWSEEEEDNGGDHYAIYPIGWRRLQFRIATANNGRRKELQQHFVLHLKLHGTLANGTKLVLSELTTAPIVVRGRSPRNFQARC"
  },
  {
    "reference": "MDILTLGWVSVLTLFTYSIAMVVWGRHGM",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein localizes to the plasma membrane-derived thylakoid membrane. The designed protein sequence is ",
    "response#1": "SNLYESLYFLVWGITLGQLLIEKEYSTPIIPAIAIPIELLTIAFACFVLPEDLKLSSNLVPALRSSWLVMHVSVVMLSYAALIMGSLLSASVLFINNSQPLQLRSSSMGVGGFKISNSYSTNNVIEPINFSHSEELDTLSYRSILVGFVLLTLGLITGAIWANEAWGTWWSWDPKETWAFISWLFYAAYLHMRISRGWQGRRPALLATSGFFVVLICYIGVNFLGVGLF",
    "response#2": "IIQHGGDPQVGNLATPINASAFSKAFINNLPGYRQGLSAQRRGLEVGMAHGYFLYGPFALLGPLRNADFAGVAGLLGTVGLISLLTLALSLYGSVGVSTPTATLTTPNPPENLGTKEGWSEFAAGFLIGGCGGALVAYGLCL",
    "response#3": "VFPAGRDPQWGNLETPVNASPLVKWFINNLPAYRPGLTPFRRGLEVGMAHGYFLFGPFAKLGPLRDAANANLAGLLGAIGLVVLFTLSLSLYANSNPPKALASVTVPNPPDAFQSKEGWNNFASAFLIGGIGGAVVAYFLTSNFL"
  },
  {
    "reference": "MSMVYFNIFMAFTVSLVGLLMYRSHLMSSLLCLEGMMLSLFVLMSMTILNNHFTLASMAPIILLVFAACEAALGLSLLVMVSNTYGTDYVQNLNLLQC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor..2. The protein should contain a Helical membrane-spanning region.3. The protein should be designed to catalyze the following reaction a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +   NAD(+).4. The protein should be designed to localize specifically to mitochondrial inner membrane, respirasome in order to perform its role in ATP synthesis coupled electron transport more effectively. The designed protein sequence is ",
    "response#1": "SSHWFMTWLGFEMNMMAIVPVLMKKYSPRSMEAATKYFLTQATASMILMLAIIINLMYSGQWTIANMENHTASMLITIALVMKLGLAPFHFWVPEVTQGIPLSSGLILLTWQKIAPLSLLYQIHSSINMELFLTMSLLSIVIGGWGGLNQTQLRKIMAYSSIGHMGWMMAIMNYNPNLSFLNLLVYILMTSSMFALLIFSSTTSTLSLSLAWNKTPIIATMSLIILLSLGGLPPLTGFMPKWMIIQELTKNNSTILPTLMAISASSHWLMIWIGFEMNMLAIIPILMKKYNPRAVEAATKYFLTQATASMLLMLGIIMNLLLTGQWAVLSTLNPIVSNTMTVALAMKLGLSPFHFWVPEVTQGIPLLSGMVLLTWQKIAPLSILYQMAPSMNPHLLMTMALMSVLVGGWGGLNQTQLRKILAYSSIAHMGWMIAVMTYSPTLMLLNLSIYITMTLGTFMLFMFNSSTTTLSLSLTWNKLPLITSLILIIMLSLGGLPPLSGFIPKWMIIHELTKNNMIITTMLMTITALLNLFFYMRLAYSSSLTMFPTTNNTKMVWQFQPMNVSFMMTSLISISIMALPLTPLIST",
    "response#2": "SSHWFMTWLGFEMNMMAIVPVLMKKYSPRSMEAATKYFLTQATASMILMLAIIINLMYSGQWTIANMENHTASMLITIALVMKLGLAPFHFWVPEVTQGIPLSSGLILLTWQKIAPLSLLYQIHSSINMELFLTMSLLSIVIGGWGGLNQTQLRKIMAYSSIGHMGWMMAIMNYNPNLSFLNLLVYILMTSSMFALLIFSSTTSTLSLSLAWNKTPIIATMSLIILLSLGGLPPLTGFMPKWMIIQELTKNNSTILPTLMAISAHWLLVWMGLEINTLAIIPLMIRQHHPRAVEATTKYFLTQATASALLLFASITNAWSLGEWSLLEMLNPTSATLVTIALALKIGLAPMHFWLPEVLQGLDLITGLILATWQKLAPFAILLQLHPMLNSNLLLFLGVSSTVIGGWGGLNQTQLRKILAYSSIAHLGWMITILHYSPNLTQLNLALYIIMTLTTFLLFKLFNSTKINSIAISTIKSPLLSIIALITLLSLGGLPPLSGFMPKWLILQELTKQDLAIPATIMALAALLN",
    "response#3": "STHWFMIWLGFEMNMMAMIPVLMKKYSPRAAEAATKYFLTQATASMILMLALIINLMYSGQWTIMNMTNNMASLLITIALTMKLGLAPFHFWVPEVTQGVSLQAGLILLTWQKIAPLAVMYQIFASINPNLLLTMALLSIMIGGWGGLNQTQLRKIMAYSSIAHMGWMTAIMIYNPNLMLLNLLLYILMTTSMFMMFMYNSTTTTLSLSLTWNKTPLMTVLMMTTLLSLGGLPPLTGFMPKWMIIHELTKNNSVILPTL"
  },
  {
    "reference": "MLKVLIPTIMLFPTIWLSSSKWLWTTTTMNSFLIAFISLTWLKWTSDTGWNASNSYMAADPLSTPLLVLTCWLLPLMILASQNHINSEPVNRQRMYITLLASLQTFMIMAFGATKIIMFYIMFEATLIPTLIIITRWGNQAERLNAGTYFLFYTLAGSLPLLVALLLLQQSTGTLSMLVLQYSDPLLLNSWGHKIWWAGCLIAFLVKMPLYGMHLWLPKAHVEAPVAGSMILAAVLLKLGGYGMMRMMVMLDPLSKQLAYPFIILALWGVIMTGLVCLRQTDLKSLIAYSSVGHMGLVAGGILIQTPWGFTGAIILMIAHGLTSSALFCLANTSYERTHSRTMILARGLQMVLPLATVWWFIANLANLALPPLPNLMGELMIITALFNWSPWTIIITGMGTLITANYSLYMFLTSQRGSIPEHITNLSPSHTREHLLMTLHLIPIILLMLKPELMWGWCN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor.2. The ideal protein would be one that plays a critical role in electron transport coupled proton transport through ubiquinone binding within respirasome. The designed protein sequence is ",
    "response#1": "MWAPDIYEGSPTPVTAFFSIAPERSISANILRVFIYGSYGATLQQIFFFCSIALRLRSTGAMANEGKASSSIGQLDYGGLYFVLVLMWNREGIQSLLIGLFIYASMDDRCFAIVSALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVIAFMSS",
    "response#2": "MWAPDIYEGSPTPVTAFFSIAPERSISANILRVFIYGSYGATLQQIFFFCSIALRLRSTGAMANEGKASSSIGQLDYGGLYFVLVLMWNREGIQSLLIGLFIYASMDDRCFAIVSALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVISALNY",
    "response#3": "MWAPDIYEGSPTPVTAFFSIAPERSISANILRVFIYGSYGATLQQIFFFCSIALRLRSTGAMANEGKASSSIGQLDYGGLYFVLVLMWNREGIQSLLIGLFIYASMDDRCFAIVSALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVISALNY"
  },
  {
    "reference": "MNTMSYCPPNITFTDIWIDHGMSKCFMDTISITIISSYLLIFGTIQLWIYQKYGTETAITLLPKNKLYIVQKFFLYFVPILSIIRIILQGTILNDTKIYGYMILTTVLTLIIYPYSIYILKIERHKLLPSVPPRGHGLVLLGFWTLAFVSENLVFVNIGKLEWWFHLNTLTDQIEMALFILRYVSNLIIFALGLKAPGITGNTNPEYHTLNNGPTLQSRYYQSRSEDTSSTWKNVWHKIKILTPFLWPKPLILQLRVIFCFGLLILGRLINLYVPIYNKKIVDSISEAPEFRWDFILIYVAFKFLQGGGTGGMGLLNNLRSFLWIRIQQYTTREIEIELFRHLHSLSLRWHLGRKTGEVLKVMDRGTDSVNNLLNYILFSIVPTIIDIIVAVAFFIVAFNNWFGLIVFLTMSLYIAATIMITEWRTKFQRRMNLADNAQKARSVDSLLNFETVKYYGAESYEVDSYRKAILEFQIAEWKSMITLNILNTLQNIIVCSGLLTGSLLCLHMVVTKQGLTIGDYVLFASYIIQLYVPLNWFGTYYRAIQKNFVDMENMFDLLREEQEIINAPGAGPLIIKRGQVEFANVTFSYTSEKIILKNISFVVPAGKTIALVGPSGSGKSTIVRLLFRFYDVEQGAILIDGQNIKTVTQDSLRRSIGVVPQDTVLFNNTIKYNIQYGRIEAIDADIISAARNADIHERILSFPNGYETQVGERGLRLSGGEKQRVAIARTILKEPAIVLLDEATSALDTQTERNIQAALSRVCANRT",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that localizes to early endosome membrane, multivesicular body membrane, melanosome membrane, extracellular region and can perform ATP hydrolysis activity, ATP binding, ABC-type transporter activity with high specificity and efficiency.2. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "FCPNGSLVDVWVNEKTSECFIETTVAGTLAMFMILFGTAQCSVYRKYSTPLSQASIPQSRLYYMQMIITFLIPLLAVAKFCFDVYLIQNGIVYGYQALYMLVMVFVFPLSAKLVLMERNSLLPSMPARGHGLVLLVFWILIFTAVNLTFISIKKENWWLQLHNIKGQIGFSLFVGNYILSFLLLLLGLKAPGIMTARDYFSLNNSSDLFLDSDRVEEVSTWSNMMRKVKMIAPFVWS",
    "response#2": "VACILLVIAGRVANVYVPLYSAKIVDSLTAVPLVFRWDLILIYIGIKFLQGGLGMGFLNNFRSFLWIRVQQYTKREVEISLFRHLHELSLKWHLSRKTGEVLRIMDRGTDSTNSLLSFILFNIGPTIIDIIVAVIFFLSYFNWMMGTVVFITMVLYIALTIIITEWRTQFQRRMNLADNNMEARSVDSMLNFETVKYYGAEEYEVQAYKDAIMVYQNEEFKTQVSLAILNTVQNLIVCTGLLAGSLLSVHFVVDVKELTVGDYVLFTVYIIQLYGPLHALGVFWGL",
    "response#3": "GMGLLNNLRSFLWIRIQQYTTREVEVELFRHLHGLSLRWHLGRKTGEVLRVMDRGTDSIDNLLNYILFSIVPTIVDILVAVLFFVSVFNKWFGLIVLTTMVFYIAATILVTEWRTKFQRRMNLADNAQKARSVDSLLNFETVKYYGAEAYEVECYREAILDYQVQEWKSMVTLNILNTLQNVIVSAGLLSGSLLCLHMVVTHQGLTVGDYVLFASYITQLYVPLNWFGTYYRAIQNSFIDMESMFTLPGQTVALVGQSGSGKSTIIRLLFRFYDVQGGCIRIDGQDITKVKQASLRAHIGVVPQDTVLFNDNIRENIRYGRISATDQEVEEAAIAADIHDRILSFPEGYDTQVGERGLKLSGGEKQRVAIARTILKAPEIILLDEATSALDTQTERNIQASLAKVCVNRTTIQ"
  },
  {
    "reference": "MKKANLAIIGATGMVGTTFIKILEERQFPVDNIYFFASEKSKGAKIQFMGKEYTVEVLNENSFDKNIDIALFSAGGNISRKFAPMAKNKGIIVVDNSSAWRMENDVPLIVPEVNEDDVSWHKGIIANPNCSTIQAVVPLKVLHDKYKIKRIVYSTYQAVSGSGMAGIEDLHRGLKGEAPTNYPHPIANNCLPHIDVFLENGYTKEEIKMIEETKKILGDQSLKITATTVRVPVENCHSISVNLELEKDFEIQKAVELLKNSHGIKVVDDPENNSYPMAINAHGTDDIYVGRIRRDYSLENGLNLWVVADNIRKGAATNTVQIAELLLKRMG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The substrate, NADP(+), phosphate binding site should be located in a region of the protein that is accessible to the ligand.2. The protein contains novel Semialdehyde dehydrogenase NAD-binding domains that confer a unique function or activity.3. The protein should be optimized for activity in L-threonine biosynthesis, Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5 pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..5. A protein with protein dimerization activity, NAD binding, aspartate-semialdehyde dehydrogenase activity, NADP binding is needed to enhance isoleucine biosynthetic process, threonine biosynthetic process, 'de novo' L-methionine biosynthetic process, diaminopimelate biosynthetic process, lysine biosynthetic process via diaminopimelate in a specific context.6. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor, Acyl-thioester intermediate to enable effective catalysis of L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH. The designed protein sequence is ",
    "response#1": "MSKVAVVGATGNVGREMLNVLAERAFPASEVVALASTRSIGKEVSFGDATLKVKALDYYDFKDTDICLMSAGGTIAKEWAPKIAAQGPIVIDNSSAWRMDREVPLVVPEVNADALNGLKPLHEAATIERINVATYQAVSGSGKEAIDELATQTANLLNGKPVECEAYPKQIAFNCLPHIDVFLDNGYTKEEMKMVWETQKIFGDDSILVNPTAVRVPVFFGHSEAVHIETREKLTEAKARELLSQFEGIEVLDDREPGGYPTAVTEAATHDPVYVGRIREDISHPRGLNLWVVADNVRDGIIANPNCSTLAPIHRKYGIERINVATYQSVSGAGRSGLEELGRQTAQLLAFQEIEPKKFQAQIAFNLIPHIDEFLPNGYTKEEMKLVWETRKILEDDSIQVNPTAVRVPVFYGHSEAVNIETKEKITPEQARELLRAAPGVEVVDEHKAGGYPTPVTHASGRDPVFVGRIREDFSHPRGLNLWVVADNIRKGAALNAVQIAETLLKPLHEAATIERINVATYQAVSGSGKEAIDELATQTANLLNGKPVECEAYPKQIAFNCLPHIDVFLDNGYTKEEMKMVWETQKIFGDDSILVNPTAVRVPVFFGHSEAVHIETREKLTEAKARELLSQFEGIEVLDDREPGGYPTAVTEAATHDPVYVGRIREDISHPRGLNLWVVADNVRGAEL",
    "response#2": "MTNVAVVGATGLVGRTMTQVLEERNFPIETLTLLASARSVGTEIMFKGKQIKVQELKPESFSGIDYALFSAGASVSKEFAPKAVQYGTIVIDNSSAFRMESDVPLVVPEVNPDDLWPLYEQIRIRRVVVSTYQAISGAGAAAMAELEQQTRDVLAGKAATPKELPHPIAFNLFSHNSAIGHDGYCEEEEKMIRETRKMFHDDDLRITPSCIRVPVPRTHSETLNIEFAEPMDEERVREILRSAPGVTLVDNREANHFPMPLEASGEDDVFVGRIRRDLSQPDGRGIDLFLCGDQLRARNAANLWPLYEQIRIRRVVVSTYQAISGAGAAAMAELEQQTRDVLAGKAATPKELPHPIAFNLFSHNSAIGHDGYCEEEEKMIRETRKMFHDDDLRITPSCIRVPVPRTHSETLNIEFAEPMDEERVREILRSAPGVTLVDNREANHFPMPLEASGEDDVFVGRIRRDLSQPDGRGIDLFLCGDQLRPAAEQPLNTPFLLKPLHEAATIERINVATYQAVSGSGKEAIDELATQTANLLNGKPVECEAYPKQIAFNCLPHIDVFLDNGYTKEEMKMVWETQKIFGDDSILVNPTAVRVPVFFGHSEAVHIETREKLTEAKARELLSQFEGIEVLDDREPGGYPTAVTEAATHDPVYVGRIREDISHPRGLNLWVVADNVRLTPGAVPAAAHLWPLYEQIRIRRVVVSTYQAISGAGAAAMAELEQQTRDVLAGKAATPKELPHPIAFNLFSHNSAIGHDGYCEEEEKMIRETRKMFHDDDLRITPSCIRVPVPRTHSETLNIEFAEPMDEERVREILRSAPGVTLVDNREANHFPMPLEASGEDDVFVGRIRRDLSQPDGRGIDLFLCGDQLR",
    "response#3": "MENIAIVGATGAVGQEILKVMTERNFPYKSLKLLASKRSAGQKITYNGEVYEVEETTEKSFDDVDIALFAGGPASRAYGRIAQSKGVIVIDNSSTFRLEPDVPLVVPEVNPEALKLKPLHEAATIERINVATYQAVSGSGKEAIDELATQTANLLNGKPVECEAYPKQIAFNCLPHIDVFLDNGYTKEEMKMVWETQKIFGDDSILVNPTAVRVPVFFGHSEAVHIETREKLTEAKARELLSQFEGIEVLDDREPGGYPTAVTEAATHDPVYVGRIREDISHPRGLNLWVVADNVRRGILSNPNCTTIMMVVALNPLHQKYGIERVFISTYQSVTGTGKKAVDQLFAESKGKYCDRIYPYPIYQNAIPQCDSFEENGYTKEELKLIREPKKIMGVKNMGVSATAVRVPVIGGHSESVQISFKKDFELDELREILKNTPGLILQDNPKNNYYPMPIHSYGKDEVFVGRLRRDLSQENSLLLWIVADNLRKGAALNAVQIAETLLKPLHEAATIERINVATYQAVSGSGKEAIDELATQTANLLNGKPVECEAYPKQIAFNCLPHIDVFLDNGYTKEEMKMVWETQKIFGDDSILVNPTAVRVPVFFGHSEAVHIETREKLTEAKARELLSQFEGIEVLDDREPGGYPTAVTEAATHDPVYVGRIREDISHPRGLNLWVVADNVRG"
  },
  {
    "reference": "MFLTEDLITFNLRNFLLFQLWESSFSPGAGGFCTTLPPSFLRVDDRATSSTTDSSRAPSSPRPPGSTSHCGISTRCTERCLCVLPLRTSQVPDVMAPQHDQEKFHDLAYSCLGKSFSMSNQDLYGYSTSSLALGLAWLSWETKKKNVLHLVGLDSL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for stability and expression in nucleus where it will participate in positive regulation of transcription by RNA polymerase II. The designed protein sequence is ",
    "response#1": "AHYWKDFHPRWPFLHRGTLKVDEAPVELLLAMITMGMHFVGDAFAYSIAVSIHSTLRFSIYTHPDFKPPASLWVYQALLIAEIFEKMTSTVEQHNLSQIFHGVTIESMQNGLSSKDTVTEKIPKNMTGTNIAQHKWHQWVDREASKRIAFFSFVLDSQHVILFGYRPLIDITSVGLPLLCDEALWNADSYEAWTSLLNENDPPHFFPVLKMFLTNEHLPPKLSPWNMMIVLHGLTTIGWILSRENLGIVENIMQNNGTNLKNWRILLKASYKFR",
    "response#2": "AHYWKDFHPRWPFLHRGTLKVDEAPVELLLAMITMGMHFVGDAFAYSIAVSIHSTLRFSIYTHPDFKPPASLWVYQALLIAEIFEKMTSTVEQHNLSQIFHGVTIESMQNGLSSKDTVTEKIPKNMTGTNIAQHKWHQWVDREASKRIAFFSFVLDSQHVILFGYRPLIDITSVGLPLLCDEALWNADSYEAWTSLLNENDPPHFFPVLKMFLTNEHLPPKLSPWNMMIVLHGLTTIGWILSRENLGIVENIMQNNGTNLKNWRILLKASYKFS",
    "response#3": "AHYWKDFHPRWPFLHRGTLKVDEAPVELLLAMITMGMHFVGDAFAYSIAVSIHSTLRFSIYTHPDFKPPASLWVYQALLIAEIFEKMTSTVEQHNLSQIFHGVTIESMQNGLSSKDTVTEKIPKNMTGTNIAQHKWHQWVDREASKRIAFFSFVLDSQHVILFGYRPLIDITSVGLPLLCDEALWNADSYEAWTSLLNENDPPHFFPVLKMFLTNEHLPPKLSPWNMMIVLHGLTTIGWILSRENLGIVENIMQNNGTNLKNWRILLKASYKFV"
  },
  {
    "reference": "MKLIKICVLKNSIFRDTFYSLFDIIYPRSENSLIFANQLRRILLGKSEGLRFLKISGFIANKNSKFSVYQKINEFSEFEEINESVFQLFLNIKNIDLYYRSCDANKRFFGTLEAYYPKTYSQDDIILSKNIKLIKKNQHIFSLSSLKLKMKFILEAKKGIGSFFTPVKRVNYIIEENKLSFSRVVLELETDKRRGCFSSTIDAFKVLNFFALNM",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be involved in DNA-templated transcription by localizing to chloroplast, DNA-directed RNA polymerase complex and exhibiting DNA-directed 5'-3' RNA polymerase activity, protein dimerization activity.2. The protein should have a fast turnover rate for a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +   RNA(n+1).3. The protein must exhibit the following characteristics: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.. The designed protein sequence is ",
    "response#1": "IKIENVLHEFSSLNGVREDVTDIVLNIKQVAIKMQGDGSRRLHISRTGPGVVTAGDIAVSGDIEVMNPELVICHLDQGAVFNAELTVDTGKGYVAAVANRPADAPIGLIPVDALYS",
    "response#2": "IKIENVLHEFSSLNGVREDVTDIVLNIKQVAIKMQGDGSRRLHISRTGPGVVTAGDIAVSGDIEVMNPELVICHLDQGAVFNAELTVDTGKGYVAAVANRPADAPIGLIPVDALYA",
    "response#3": "RIDGVLHEFTTIPGVKEDVTDVILNLKELVVSSEEDEPVTMYLRKQGPGEVTAADIVPPAGVTVHNPDLHIATLNGKGKLEIELVVERGRGYVPAVQNKQTGAEIGRIPVDSIYR"
  },
  {
    "reference": "MKKTGKNLAAIILAAGKGERMKSSLPKVLHPICGRPMLAYILDLVEELNIKNTVAVLGYGYERVKPHLKPWIKVVIQRRLLGSADAVKVGLSALKNFSGTILVLYADNPLLKKETIKKLIQYHQENEPAVTLLTGKVKDPSGYGRILRDANFNICGIIEEKDADEFQKGIKEINTGIACFDKDSLIQALKKVRPDNRKKEYYLTDTVGILYKKGLLIDNVRLLDIKEALGVNSRVDLAKANRIIQTRINEDLMKQGVTIIEPDSTFVSYGTRIGKDTIIYPFTVIESGVKIGKRCHIGPFAHIRSGTRLKDDVVVGNFLEVSRSRISSGTIAKHFGYIGDSEIGRFVNIGAGCVTANFDGKTKNKTIIKDGAFIGSDTVLIAPVSIGRCARTGAGAVVVKNQNVGDGETVVGIPAKSIKIKTSYK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a binding site that is specific to the target UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA, Mg(2+) ligand.2. A protein that can perform glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity in cytoplasm to support regulation of cell shape, lipid A biosynthetic process, UDP-N-acetylglucosamine biosynthetic process, cell morphogenesis, peptidoglycan biosynthetic process.3. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.. The designed protein sequence is ",
    "response#1": "MAAGVTLVAPQTVFFSADTEIEADVVVEPNVVFGPGVSIAAGTVIHAFSHVEGARIGADVSIGPFARLRPGTEIEPKARVGNFVETKNLKLAEGAKVNHLTYLGDASVGPRSNIGAGTITCNYDGFNKYRTAIGADAFIGSNSSLVAPIVIGDGAYVGSGSVVTEDVPDEALAIGRGRPALSIVVPEGVTIIDPQTTYIDAGVEIGADTVIWPGTILRGRTVIGEDCVVGPQADITDSVIGRGVTVRHSVLLEAEVGDGCTIGPYAHLRPGSKLAAGCRIGDFVELKNAKLGEGTKVPHLSYVGDAIVGRDVNFGCGAITVNYDGYNKSVTEIGDRAFIGSNVNLIAPVKIGEDAYVVAGSTITHDVKPNDMAIARERDAFGNGPFYMQNPEGVTMRDPSTVYVDHGVKIATDVVLDPGVILNGSTMIGEGSVIGPDTTIVDSAIGRDCSVRRSVIEGSVVEDGASIGPYSHLRSGSHVGAGAEVGNYAEVKNSRLGARVKQHHMSYLGDAEIGEGTNVGAGTITANWDGGAKNRTRVGRNAFLGVDTMLVAPVDVGDGAMTGAGAVVTKDVPPGKLAVLSCN",
    "response#2": "MRLAVVLAAGQGTRMKSKLYKVLHPVCGKPMVEHVVDMISDLGVTQTVTIVGHGAEKVQETLGERTSYALQSEQLGTAHAVMQAESELAGKKGTTIVICGDTPLIKKETMEALLAHHQETKAKATILTGKPSNPTGYGRIIRGEDGFVKRIVEQKDATAEEQKVNEINSGTYCFDNELLFDLLKKVDNSNAQGEYYLPDVIELLTQQNDIVSAYAVPDFEETLGVNDRAQLARAGEILRKRLNEALMRNGVTIEDPATTYVDTDVTVGADTVLHPGVTLEGRTQIGSACEIHSGVRIVDSTIADGVTINNHCVITDARVAAGARIGPFAHLRPEADIRRDAKIGNFVEVKKAVVGAGSKANHLAYLGDATVGEGVNVGAGTIICNYDGTTKHHTVIEDGAFIGSDTQLIAPVTVGKGAYVASGSSITDDVPAGSLAVARGRQVNKENYVERVQLATCEALFQARKRERAMRDGVTLQAPETVFFAHDTEIGRDVTIEPNVMFGAGVKVADGAVIHAFSHLEGATVGQGATVGPFARLRPGTDLGPEVKIGNFVETKAATIGAGAKVSHLSYIGDARVGAEANIGAGTITCNYDGYFKSLTEIGAQAFIGSNSALVAPVKIGRGAYVGSGSVITKDVPDDALAVGRGRGVPARVKK",
    "response#3": "VLVLAAGPGTRMRSDTPKVLHTIAGRSMLSHSLHAVAKVAPRHLVVVLGHDHQRIAPLVAGLAESMGRTIDVALQDRPLGTGHAALCGLSALPDDYAGIVVVTSGDTPLLDSDTLAGLIAAHTAAPAAVTVLTTEVPDATGYGRIVRDPDGRVLRIVEERDATPEQRALREINSGVYVFRADALRAQLPQVGRANSQGELYLTDVLGILVAEGHPVGAHLAPDGLETQGVNDRVQMARLDAEVRRRIVEWWLRSGVTLLAPETVQIRGVVECGRDVVLDAQVTLEGQVRLGEGVTIGQGCVVRDSELAPGSRLEPYCVVEGVRSTGPCSVGPFARLRPGTQLAAGVKIGNFVEVKKARFAAGAKASHLSYVGDAQVGERANLGAGTITCNYDGANKHHTEIGADAFIGSNSALVAPVSIGAEATIGAGSVIGKDAPAAQLTLTRSRGPCVIEGARVQLATCEALFQARKRERAMRDGVTLQAPETVFFAHDTEIGRDVTIEPNVMFGAGVKVADGAVIHAFSHLEGATVGQGATVGPFARLRPGTDLGPEVKIGNFVETKAATIGAGAKVSHLSYIGDARVGAEANIGAGTITCNYDGYFKSLTEIGAQAFIGSNSALVAPVKIGRGAYVGSGSVITKDVPDDALAVGRGRRTKVLHEVAGRALVAHVLASAADAGSDAVALVVGRDAEAVTKLGNALGHPAQSFEQTERLGTAHAVLAARAEIAEGYDDVLVLFGDTPLLSAETLRRARIQLADGAAVCVVGFEADDPAGYGRLLLDGGQLLAIREGRK"
  },
  {
    "reference": "MSRRTRCEDLDELHYQDVDSDLLEQRDNRCKVKWTHEEDEQLRALVRQFGQQDWKFLASHFPNRTDQQCQYRWLRVLNPDLVKGPWTKEEDQKVIELVKKYGTKQWTLIAKHLKGRLGKQCRERWHNHLNPEVKKSCWTEEEDRIICEAHKVLGNRWAEIAKMLPGRTDNAVKNHWNSTIKRKVDTGGFPAESRDCKPVYLLLELEDKEQHQGVQPVDGQGSLVSSWPLVPSIVKEESSEEEIAIAATSAKELGHEPVPADLGEVRTPEPPESLKREYQEFSSPETSLPYKWVVEAANLLIPAVGSSLSEALDLIESDPDAWCDLSKFDLPEEPSTEGSVVSSPVQPQTSQQQQEEALQSSQQAATPGPSVTEYRLDGHTISDLSRSSRGELIPISPSTEFGGSGIGTPPSVLKRQKKRRVALSPVTENSASLSFLDSCNSLTPKSTPVKTLPFSPSQFLNFWNKQDTLELESPSLTSTPVCSQKVVVTTPLHRDKTPLHQKYPSSEVLPDQKYSMDNTPHTPTPFKNALEKYGPLKPLPQTPHLEEDLKEVLRSEAGMELIIEDDMRPEKQKRKPGLRRSPIKKVRKSLALDIMDEDGKLMSSTMPKPLSLPTSVTPSSCGFTSPGSKEGNSLLNQGFLQAKPEKVVAAQKTRSHIPTPAPMTHAWKTVACGGTKDQLFMQEKARQLLSRLKSSHTSRTLILS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should prioritize the formation of helix-helix-strand-helix elements.2. For general function, the protein need meet that Transcription factor involved in the regulation of cell survival, proliferation, and differentiation; Transactivates the expression of the CLU gene .3. A protein that can perform sequence-specific double-stranded DNA binding, DNA-binding transcription factor activity, RNA polymerase II-specific, RNA polymerase II cis-regulatory region sequence-specific DNA binding, DNA-binding transcription activator activity, RNA polymerase II-specific in nucleoplasm, Myb complex, nucleus to support mitotic cell cycle, positive regulation of neuron apoptotic process, mitotic spindle assembly, cellular response to leukemia inhibitory factor, regulation of DNA-templated transcription.4. The H-T-H motif domain within the designed protein should be capable of recognizing and binding to a range of DNA sequences.5. The protein should exhibit Polar residues compositional bias.6. The designed protein have HTH myb-type domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "IDSFLNTSTQHENLEMEHQSLTSTPICASQKNTITTSLLRDHALRLQKENHLLRTPAIKRSILNSTPRTPTPFKNALAAQEFKHGPLKLLHHTPLHLAEDIQEVIKQETDESGIVHDHCNTEEPLLKRIKQEVESPTHKVGNLYFSSYWEGESLNAQLGSRGFLPANPLERLQGLFMASFEAIEHHHAQVVIYQDEAQRLASQPRFSYIEDRNKQQRKMWVDVLNQGIEEGYFRPDLDVDLVYRFIRDTTWVSVRWYRPGGPLTAQQVGQQYLAIVLGGKSSWTEEEDRTIYEAHKRLGNRWAEIAKLLPGRTDNAIKNHWNL",
    "response#2": "LVTKWKNWEKALLRKSVVSDRLQRLLQPKLLKLEYLHQKQSKVSSELERQALEKQGREAEKEIQDINQLPEEALLGNRLDSHDWEKISNINFEGSRSAEEIRKFWQNSEHLVTKWKNWEKALLRKSVVSDRLQRLLQPKLLKLEYLHQKQSKVSSELERQALEKQGREAEKEIQDINQLPEEALLGNRLDSHDWEKISNINFEGSRSAEEIRKFWQNSEHKTSWTEEEDRIIYQAHKRLGNRWAEIAKLLPGRTDNAVKNHWNPLVTKWKNWEKALLRKSVVSDRLQRLLQPKLLKLEYLHQKQSKVSSELERQALEKQGREAEKEIQDINQLPEEALLGNRLDSHDWEKISNINFEGSRSAEEIRKFWQNSEHFHPSINKQEWSREEEERLQAIAAAHGHLEWQKIAEELGTSRSAFQCLQKFQQHGANPLERLQGLFMASFEAIEHHHAQVVIYQDEAQRLASQPRFSYIEDRNKQQRKMWVDVLNQGIEEGYFRPDLDVDLVYRFIRDTTWVSVRWYRPGGPLTAQQVGQQYLAIVLGGLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARLGKTRWTREEDEKLKKLVEQNGTEEWKVIASFLPNRTDVQCQHRWQKVLNL",
    "response#3": "LVTKWKNWEKALLRKSVVSDRLQRLLQPKLLKLEYLHQKQSKVSSELERQALEKQGREAEKEIQDINQLPEEALLGNRLDSHDWEKISNINFEGSRSAEEIRKFWQNSEHTKGQWTPEEDEVLCKAVERFQGKNWKKIAECFKDRTDVQCLHRWQKVLNEGSPNKWTEEEDQKLFQLVSIYGEKKWKRISAEMGGQKTGAQCAQHWKRVLSKENPGINKNAWTQEEELTLIRAHQIYGNKWAELMKFLPGRSDNSIKNHWNSSGKGPWTKEEDQRVIELVHKYGPKRWSVIAKHLKGRIGKQCRERWHNHGKRGNFTEEEDEIIIKLHSLLGNKWSLIAGALPGRTDNEIKNYWNTHIKPLVTKWKNWEKALLRKSVVSDRLQRLLQPKLLKLEYLHQKQSKVSSELERQALEKQGREAEKEIQDINQLPEEALLGNRLDSHDWEKISNINFEGSRSAEEIRKFWQNSEHIKWTEEEDQKLFQLVSIYGEKKWKRISAEMGGQKTGAQCAQHWKRVLSPDEKKGKFTEDEKVSIKKALENYQKIHRMSSFDLVELVMTKTLQATDKETVREFWKDIAASVPGRPILNVQPFVRRMLDSGQKS"
  },
  {
    "reference": "MENQSKSYTANSGGKCPFHHGTMEHTAGRGTQNQDWWPNQLNLDLLRQHSSKSNPLDPEFNYPKAFSKLDYEGLKADLRQLMTDSQDWWPADYGHYGPFFIRMAWHSAGTYRITDGRGGSGTGSQRFAPLNSWPDNANLDKARLLLWPIKQKYGQSISWADLMILAGNVALESMGFKTYGFAGGREDIWEPEKDVYWGSESEWLGDKRYTGDRELEQPLGAVQMGLIYVNPEGPNGNPDPVAAARDIRETFKRMAMDDEETVALIAGGHTFGKTHGAADPEKHVGREPAGASIEEQSLGWKNSYGTGKGKDTITSGLEGAWTDTPTQWSHKYFENLFNYDWELTKSPAGAHQWKPKNGAGEGTIPDAHDPSLKHAPFMLTTDLSLRFDPAYEKISKRFLEHPEEFEDAFARAWFKLTHRDMGPKQRYLGPEVPQEDLIWQDPIPNSKKVDLSEGDLQLLKSEILSSGLSLSTLVKTAWAAASTYRDSDKRGGVNGARIRLKPQAEWEVNNPIQLKKTLTELEKIQNKFNANERGKSISLADLIVLAGNTAIEQAAKGAGHEITIPYEAWRNDATEEHTDFESFEYMRPLADGFRNYKVEGIKVKSEDLLIDKAQLLTLTPKELTVLVGGMRALGANWDDSETGIFTDRKGQLTNDFFKNLLTPGYQWKAEGEDEEYFTALHPKTGSRCRASRVDLIFGSNSELRTIAEVYACDDAENKFVQDFCAAWNKVMNLDKF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein with the reaction AH2 + H2O2 = A + 2 H2O, the Proton acceptor must be optimized to ensure efficient catalysis.2. The designed protein must possess catalase activity, metal ion binding, heme binding to support hydrogen peroxide catabolic process, response to oxidative stress under certain conditions.3. The presence of heme b is necessary for the protein to function in the desired environment.4. The designed protein must have at least one Plant heme peroxidase family profile domain.5. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.6. For general function, the protein need meet that Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.7. The heme b binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "AFARAWFKLTHRDMGPLARYLGPLVPKEELLWQDPVPAVTHELISDADIEALKAKILASDVTPAQFIATAWGAAASFRGSDKRGGANGGRIRLEPQKSWEVNQPQVLAKVLTVLEGIQSDFGKPVSIADLVVLAGTAAVEAAAKKAGFDVKVPFTPGRTDASQEQTDVVSFAFLEQKADAFRNYLTPGHKLRAEELMIDRAQLLTLTAPEMAVLLGGLRVLGANHRGSKNGVFTDQPGTLTNDFFVNLLDMATEWKPASTGEGLFEGLDRATGEIKWTATRVDLVFGSNSQLRALAEVYAAE",
    "response#2": "TFARAWFKLLHRDMGPKVRYLGPEVPAEELIWQDPVPAVDYELFHDEVAELKNRILSSGLTVSELVKTAWASASTYRHTDMRGGANGARIRLAPQKDWEANEPEQLAKVLGVYEDIQSKLTVRVSLADLIVLGGSAAIEKAAKDAGFDVTVPFTPGRGDATEEQTDAETFDVLEPVSDGFRNYQKKEYSTSPEEMLVDKAQLLGLTAPEMTVLLGGMRVLGTNHGGTKHGLFTDRPGILTNDFFINLLDMRTEWKSIGFNQYEGRDRKTGEVIRTASRFDLLFGSNSELRALAEVYAQAKAWFKLTHRDMGPLSKYIWEKEPKETLIWQDPIPSVDHKLIGEKEIKSLKGKILGSGLTIPELVRTAWAAAASFRSTDMRGGANGGRLRLSPQREWTVNNPDELKKVLKELTEIQREFNESGKKVSLADVIVLGGVAAVEEAAKKAGVKVSVPFTPGRMDASQESTDVKSFAVLEPKADAFRNYYGDGNYLSPTEALVDRANMLSLTVPEMTVLVGGLRALNANSANSKHGVLTQRPGVLSNDFFVNLLDMSTKWQKSSDNAAVYEGIDRKTGAKKWSATSVDLIFGSHSELRSVAEVYASV",
    "response#3": "AKAWFKLTHRDMGPLSKYIWEKEPKETLIWQDPIPSVDHKLIGEKEIKSLKGKILGSGLTIPELVRTAWAAAASFRSTDMRGGANGGRLRLSPQREWTVNNPDELKKVLKELTEIQREFNESGKKVSLADVIVLGGVAAVEEAAKKAGVKVSVPFTPGRMDASQESTDVKSFAVLEPKADAFRNYYGDGNYLSPTEALVDRANMLSLTVPEMTVLVGGLRALNANSANSKHGVLTQRPGVLSNDFFVNLLDMSTKWQKSSDNAAVYEGIDRKTGAKKWSATSVDLIFGSHSELRSVAEVYASSRGHILVNKAAKAWFKLTHRDMGPLSKYIWEKEPKETLIWQDPIPSVDHKLIGEKEIKSLKGKILGSGLTIPELVRTAWAAAASFRSTDMRGGANGGRLRLSPQREWTVNNPDELKKVLKELTEIQREFNESGKKVSLADVIVLGGVAAVEEAAKKAGVKVSVPFTPGRMDASQESTDVKSFAVLEPKADAFRNYYGDGNYLSPTEALVDRANMLSLTVPEMTVLVGGLRALNANSANSKHGVLTQRPGVLSNDFFVNLLDMSTKWQKSSDNAAVYEGIDRKTGAKKWSATSVDLIFGSHSELRSVAEVYASVRGSTLLDKEAAKAWFKLTHRDMGPLSKYIWEKEPKETLIWQDPIPSVDHKLIGEKEIKSLKGKILGSGLTIPELVRTAWAAAASFRSTDMRGGANGGRLRLSPQREWTVNNPDELKKVLKELTEIQREFNESGKKVSLADVIVLGGVAAVEEAAKKAGVKVSVPFTPGRMDASQESTDVKSFAVLEPKADAFRNYYGDGNYLSPTEALVDRANMLSLTVPEMTVLVGGLRALNANSANSKHGVLTQRPGVLSNDFFVNLLDMSTKWQKSSDNAAVYEGIDRKTGAKKWSATSVDLIFGSHSELRSVAEVYAS"
  },
  {
    "reference": "MSASHTAPLSIVILAAGRGTRMKSRLPKVLHGLAGRPMLGHVLTAARALDPLKTVVVTGHGADEVDAHLRRRFEPAFTAGQLATVLQEPQQGTGHAVQQALPQLPPGDGTTLILYGDVPLTEPATLQQLVAAAAPSAQAPQGQLALLTIALADPSGYGRIVRDEAGAVRAIVEHKDASDAQRAIRETNTGLMAVPTVHLRQWLGQLRNDNAQGEYCLTDIVALAVADGVPVTAVAIDDEIQVAGVNSPAQLAALERAYQGRIASQLLEQGVRLADPARLDVRGALVCGQDVEIDVNCVFHGNVTLGDGVRIGANCVIGNAVIEAGAVIHPYTHIDGEQAGVNVGPGALIGPFARLRPGARLGAEVHIGNFVEVKNSTLADGAKANHLAYLGDATVGARVNYGAGSITANYDGANKHRTVIEDDVHVGSNCVLVAPVTIGAGGTVGGGSTITKDTPAGALSVARGRQLSIPNWSRPAKKPKA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.2. The designed protein should be able to compete with endogenous proteins or inhibitors for UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA binding.3. A protein should be able to manipulate the activity of enzymes in the Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway, either by activating or inhibiting their function.4. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc)5. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.6. A protein that can perform UDP-N-acetylglucosamine diphosphorylase activity, glucosamine-1-phosphate N-acetyltransferase activity in cytoplasm to support lipid A biosynthetic process, peptidoglycan biosynthetic process, cell morphogenesis, cell wall organization. The designed protein sequence is ",
    "response#1": "MAAGQGTRMRSALPKVAHRLAGKPLVRHIIDAALEAGVEDCVVVVGAGATADAVRAAAGTGVRFAVQVEQLGTGHAVACAREAAGGAPYVLIMNGDVPLVLPSTLRRLMAAVSSERAEGFGLVAARIAGDAGRAARLVGFRPSDPARLQREIVEVVAADPGDESLRERVNEVNAGVYLVPVAWLREWLPRLSCGNAQGEYYLTDLVGLAADAGLPVRAVEVADETEVLGVNDRAQLAAAEARLRADNARRLMAAGVWMLDPGRVYIDVSVRLEAGVRILPGVHLEGSTSVAEGAELGPDCFIRDSRIGPGARIWYSVVRGVSVGTDAQVGPYASLRPGVELGDSAKAGTFVEVKNSVVGARAKVPHLAYVGDTDIGEEANVGAGTITANYDGYNKHRTRIGSRAQIGSNTVLVAPVEVGAEAYTGAGSAITRDVPDGALGVERTSQRHIPDWQRPKK",
    "response#2": "MAAGQGTRMRSALPKVAHRLAGKPLVRHIIDAALEAGVEDCVVVVGAGATADAVRAAAGTGVRFAVQVEQLGTGHAVACAREAAGGAPYVLIMNGDVPLVLPSTLRRLMAAVSSERAGDPGRMALLVAELADPTGYGRIVRDAEGGVERIVEQKDASAEELAVDEINAGIYAFDGALLAEAVARLTTDNAQGERYLTDVLGIARADGRRVGAFVAGDWREIAGVNDRAQLAEARRLLNDRLLEEWMRSGVTIVDPLTTWVDVDVELAPDVTLLPGTQLYGATVVAEGATVGPDTTLTDVEIGPGAMVVRTHGSLAVIGARASVGPFAYLRPGTVLGDDGKIGTFVETKNAQIGAGSKVPHLSYVGDATIGEQTNIGASSVFVNYDGVRKHHTTIGSYARTGSDTKFIAPVTVGDGAYTGAGSVIKLDVPPGALAVSSGTQRNIEGWVLRKRAGSRAAEAAEKARKEAEAEQAEN",
    "response#3": "MAAGQGTRMRSALPKVAHRLAGKPLVRHIIDAALEAGVEDCVVVVGAGATADAVRAAAGTGVRFAVQVEQLGTGHAVACAREAAGGAPYVLIMNGDVPLVLPSTLRRLMAAVSSERAADPAGLALLTVELPDPTGYGRIVRDGGGRVAAIVEQKDASPEQLAVREVNTGILAVPARHLSQWLAGLRNDNAQGEYYLTDIVAMAVADGVAVHTVQPADAWEVEGANDKVQLARLERAHQARAADRLLTAGATIQDPATTWIDVTATLAPDVTVLPNTHILRATAVAAGATIGPDTTLEDCEVGENAVVRRADATLAVIGAGATVGPFAYLRPGTKLAAGGKIGTFVETKNSTIGEGSKVPHLSYIGDTTIGRGVNLGAGAITANYDDIAKHRTEIGDEVHSGSHNVFVAPVRIGDGAKTGAGAVIRKDVPAGALALSVAPQRHIAGWQRPVKKK"
  },
  {
    "reference": "MGDMTNSDFYSKNQRNESSHGGEFGCSMEELRSLMELRGTEAVVKIKETYGDTESICRRLKTSPVEGLPGTAPDLEKRKQIFGQNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIISLGLSFYHPPGESNEGCATAQGGAEDEGEAEAGWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVVRAGQVVQIPVAEIVVGDIAQIKYGDLLPADGLFIQGNDLKIDESSLTGESDQVRKSVDKDPMLLSGTHVMEGSGRMVVTAVGVNSQTGIIFTLLGAGGEEEEKKDKKGVKKGDGLQLPAADGAAPANAAGSANASLVNGKMQDGSADSSQSKAKQQDGAAAMEMQPLKSAEGGDADDKKKANMHKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILVLYFTVDTFVVNKKPWLTECTPVYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQAYVGDVHYKEIPDPSSINAKTLELLVNAIAINSAYTTKILPPEKEGALPRQVGNKTECGLLGFVLDLRQDYEPVRSQMPEEKLYKVYTFNSVRKSMSTVIKMPDESFRMYSKGASEIVLKKCCKILSGAGEPRVFRPRDRDEMVKKVIEPMACDGLRTICVAYRDFPSSPEPDWDNENDILNELTCICVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAIKCGIIHPGEDFLCLEGKEFNRRIRN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that can perform calcium-dependent ATPase activity, glutamate receptor binding in parallel fiber to Purkinje cell synapse, photoreceptor ribbon synapse, postsynaptic density membrane to support cellular calcium ion homeostasis, serotonin metabolic process.2. The protein must exhibit the following characteristics: Regulates Ca(2+) levels in the vestibular system, where it contributes to the formation of otoconia ; ATP-driven Ca(2+) ion pump involved in the maintenance of basal intracellular Ca(2+) levels in specialized cells of cerebellar circuit and vestibular and cochlear systems; Plays an essential role in hearing and balance; Provides for fast return of Ca(2+) concentrations back to their resting levels, ultimately contributing to long-term depression induction and motor learning .3. Target a Polar residues, Basic and acidic residues compositional bias in the protein's composition for improved properties.4. The protein with the reaction ATP + Ca(2+)(in) + H2O = ADP + Ca(2+)(out) + H(+) + phosphate, the 4-aspartylphosphate intermediate must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "TEHSMKLESLLSMYETSLEKGLSENIVARNLERDGLNALTPPKQTPEWVKFCKQMFGGFSMLLWIGAILCFFAFLF",
    "response#2": "YDVKEELGKGAFSVVRRCVHKTTGLEFAAKIINTKKLSARDFQKLEREARICRKLQHPNIVRLHDSIQEESFHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESIAYCHSNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVNDSEAWHGFAGTPGYLSPEVLKKDPYSKPVDIWACGVILYILLVGYPPFWDEDQHRLYAQIKAGAYDYPSPEWDTVTPEAKSLIDSMLTVNPKKRITADQALKVPWFDKNADGKLTLQELVETQKQDIVRVTQTLLDAISCKDFDTYTRLCDTSMTCFEPEALGNLIEGIEFHRFYFDGNRKNQVHTTMLNPNVHIIGEDAACVAYVKLTQFLDRNGEAHTRQSQESRVWSKKQGRWLCVHVHRSTQK",
    "response#3": "MLVKGSVFLEQLGSVQMIAFDKTGTVTKGQPAVETIRIAEGFSEAEVLEAVYAIETQSSHPLAQAITAYAESRGVNQSGYISIEETSGFGVMAEVSGAKWKVGKAGFIGEEMAAQFMKQTASDVIQSGHTIVFVKKDDQIAGCIALKDQIRPEAKEVMEELNRLGIKTAMLTGDHEDTAQAIAKEAGMTTVVAECLPDQKVNEIKRLKEEFGTIAMVGDGINDAPALKAADVGIAMGGGTDVALETADMVLMKNDLKKLVNMCRLSRGDLNGDGLVDFEEVRNVIATSGRAVEACGDINSLVLDKTGTITFGNRMADEFIPVNSYTVEDVARIAKLASLFDETPEGKSIVKLAEKYKILADVNLNQAEGVEFSAKTRMSGTNLPNGKQVRKGAVDAIKGFIRSRGGSIPSDVDVAYERVSLLGGTPLAVCQDNEIFGVIYLKDIVKSGLRERFEQLRRMGVKTIMLTGDNHITASVIAQEAGVDDFIAEATPEDKIDVIRNEQSQGKLVAMTGDGTNDAPALAQANVGLAMNSGTQAAKEAANMVDLDSDPTKLIDLVTIGKQKQDIVRVTQTLLDAISCKDFDTYTRLCDTSMTCFEPEALGNLIEGIEFHRFYFDGNRKNQVHTTMLNPNVHIIGEDAACVAYVKLTQFLDRNGEAHTRQSQESRVWSKKQGRWLCVHVHRSTQK"
  },
  {
    "reference": "MSTQFNFVSVKKKSNIHFDGCSISHVIELKDGTKKTLGVILPTEHPLVFETQVAERIEIVSGKCLVQIGNNHESQTYYAGESFYVPKNTQFKIITDDIIDYVCHLEDR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Also catalyzes the reverse reactions.; Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates; Catalyzes the phosphorolysis of diverse nucleosides, yielding D-ribose 1-phosphate and the respective free bases2. The designed protein must be optimized for guanosine phosphorylase activity, purine-nucleoside phosphorylase activity, thymidine phosphorylase activity, pyrimidine-nucleoside phosphorylase activity. The designed protein sequence is ",
    "response#1": "MXNLXWXKR",
    "response#2": "LVLL",
    "response#3": "MSILSGR"
  },
  {
    "reference": "MRVKGIWKNYQHWLRWGTMLLGMLMICSATENLWVTVYYGVPVWKEATTTLFCASDAKAYDTEAHNVWATHACVPTDPNPQEILLKNVTENFNMWSNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDYNGTCAKDTSKNNVTCGNDTVDNSSIGEVKNCSFNITTGIRDRVQKDYALLYKLDLVQIDEESDGKDDTNSSSNDTNSFNSSSSSSNRHRYRLINCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNDTKFNGSGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVIIRSANLSDNTKTIIVQLNKSVEIKCIRPNNNTRRSVHIGPGSAFYTTGEIIGNIRQAHCNISGTDWNNTLTWIAIKLREQFPNTTIAFNHSSGGDPEIVMHTFNCGGEFFYCDTTKLFNNSWNGTWSNNSTANITITLPCRIRQIVNMWQEVGKAMYAPPIRGIINCTSNITGLLLTRDGGSTTSNNTINKTETFRPGGGDMRDNWRSELYKYKVVQIKPLGIAPTQAKRRVVQREKRGIGALFLGFLGAAGSTMGAASVTLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLALERYLRDQQLLGIWGCSGKLICTTTVPWNTSWSNKSLHEIWDNMTWMQWEKEIDNYTNVIYNLLEDSQNQQEKNEQELLELDKWASLWNWFDITNWLWYIKIFIMIVGGLIGLRIVFSVLAIVNRVRKGYSPLSFQTHLPAQRGPDRPGGTEEEGGER",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Include a signal peptide as an essential component of the protein.2. The protein design should emphasize Polar residues compositional bias.3. The ideal protein would be one that plays a critical role in positive regulation of establishment of T cell polarity through structural molecule activity within host cell plasma membrane, host cell endosome membrane, virion membrane.4. The designed protein should contain at least one Di-leucine internalization motif, YXXL motif; contains endocytosis signal motif to achieve its intended function.5. The protein must exhibit the following characteristics: The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. The designed protein sequence is ",
    "response#1": "MRVKEIRKNYQHLWRWGTMLLGMLMICSAAGKLWVTVYYGVPVWREAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQEMVLENVTENFNMWKNDMVDQMHEDIINLWDQFLKPCVKLTPLCVTLNCGNITTTTDPKVKNTNVTGSNEEEMRNCSFNITTGIRDKKQKAYALFYKLDIVQINKNNASNNESEYILINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCNDMRFNGTGLCQNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENISDNAKTIIVQLNQSVSINCTRPNNNTRKSIHLGPGQAFYATGEIIGDIRQAHCNVSKSAWNKALKGVADKLREHWKNSTIIFNQSSGGDLEITTHSFNCGGEFFYCNTTSLFNSTWPSNSTGPDNETEENSTITLPCRIKQVVNVWQRVGQAIYAPPIQGLIKCNSNITGILLTRDGGQNTSSNETFRPGGGNMKDNWRSELYKYKVVKLEPLGVAPTRAKRRVVEREKRAVGMGAVLFGFLGAAGSTMGAASITLTVQTRQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLQDQQLLGIWGCSGKLICTTAVPWNNSWSNKSLNQIWDNMTWMQWEKEISNYSNEIYRLIGESQIQQEKNEKELLELDKWASLWNWFDITSWLWYIKIFIIIVGGLIGLRIVFAVLSIANRCRQGYSPLSLQTRFPTQRGPDRPE",
    "response#2": "MKVMGIQRNCQQWWIWGILGFWMLMICNGNLWVTVYYGVPVWRDANTTLFCASDAKAHETEVHNVWATYACVPTDPNPQEIHLANVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTNSTLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLGNVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDDLGNATLGDATNNNNSSELIMEKGEIKNCSFNITTSIRDKVQREYAYFYKLDVIPIDNDNTSYRLISCNTSVITQACPKISFEPIPIHYCAPAGFAILKCNDKKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEKEVVIRSENFTNNAKNIIVQLKESVVINCTRPNNSTRKGIHMGPGGALYTTGSIIGNIRQAHCNLSITQWNNTLQQIVTKLREQFGNKTTIVFNHSSGGDPEIVMHSFNCGGEFFYCNTTKLFNSTWNDNSTWNVNGTGNDNDTIILPCRIKQIVNMWQEVGKAMYAPPIRGLIKCSSNITGLILTRDGGNNITNETEIFRPAGGDMKDNWRSELYKYKVVQIEPLGVAPTKAKRRVVQREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLKAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKIICTTAVPWNRTWSNKSYEEIWGNMTWTEWEREISNYTDIIYGILTESQNQQDRNEKDLLELDKWASLWNWFDISRWLWYIRIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQT",
    "response#3": "MRVKGMQKNWQHLWTWWTLILGLVIICSASDNLWVTVYYGVPVWKEAETTLFCASDAKAYKAEAHNIWATHACVPTDPSPQEIVLENVTENFNMWKNNMVEQMQEDIISLWDQSLKPCVKLTPLCVTLNCTTNSTF"
  },
  {
    "reference": "MGLDNSKNTAKFFDLPKAVHGMNGTTPVNGFKATALSKASRTMTKTSALSQFLEAYRELEGYKNGRAIKVDGQTLSIAAVAAAARYNAAVELDESPLVKERVRKSQLAIANKVSTGASVYGLSTGFGGSADTRTDKPMLLGFALLQHQHVGILPTSTEPLDVLPLQDANNTSMPEAWIRGAILIRMNSLIRGHSGIRWELIEKMRELLAANVIPVVPLRGSISSSGDLSPLSYIAGTIIGNPSIKVYHGPSKSGIRQIGSSKDVLALHNIEPFPLESKEPLGILNGTAFSASVAALALNEAIHLVLLAQVCTAMGTEALIGTRASHAPFIHATARPHPGQVECAENIWNLLDGSKLAQLEEHEVRLEDDKYTLRQDRYPLRTSPQFLGPQIEDIISAFQTVTQECNYLPATDNPLIDGETGESHHGGNFQAMAVTNAMEKTRLALHHVGKLLFSQSTELVNPAMNRGLPPSVAATDPSLNYHAKGLDIATAAYVAEATPGPTHIQSAEMHNQAVNSLALISARATITSLEVLTSLIASYLYILCQALDLRALQREFLPGLDIIIREELRSSFGSFLSSEQMEKLQQNLTSAFEDHLDKTTTMDNTDRMTTMAATSSSVLLQFFTDSGASVPPSSCDLLSSVSSFQSSVATRSSVLMDDLRKEYIFGDRGPTPASQYIGKTRPVYQFIRTTIGVRKHGSENYNKFYNGLGVEDVTIGQNISRIYESIRDGKMQSIIVSLFD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Catalyzes the non-oxidative deamination of L-phenylalanine to form trans-cinnamic acid and a free ammonium ion .2. Ideal protein should have a binding affinity for key enzymes in the Phenylpropanoid metabolism pathway to enhance the overall efficiency of the pathway.3. The protein design must be focused on enhancing the efficiency of cinnamic acid biosynthetic process.4. The designed protein should have a binding site that is specific to the target (E)-cinnamate ligand. The designed protein sequence is ",
    "response#1": "GVLSPYTYTLRETGPEDVFIKIIYCGICHTDIHQIKNDLGASNYPMVPGHEVVGEVVEVGSDVTKFKVGDCVGDGTIVGCCKTCRPCKADVEQYCNKKIWSYNDVYTDGKPTQGGFSGHMVVDQKFVVKIPDGMAPEQAAPLLCAGVTVYSPLTHFGLKEISGLRGGILGLGGVGHMGVKLAKAMGHHVTVISSSDKKKEEAIDHLGADAYLVSSDATQMQEAADSLDYIIDTVPVFHPLEPYLSLLKLDGKLILMGVINTPLQFISPMVMLGRKAITGSFIGSMKETEEMLDFCNEKGITSTIEVVKMDYINTAFERLEKNDVRYRFVL",
    "response#2": "GVGHMGVKLAKAMGHHVTVISSSDKKKEEAIDHLGADAYLVSSDATQMQEAADSLDYIIDTVPVFHPLEPYLSLLKLDGKLILMGVINTPLQFISPMVMLGRKAITGSFIGSMKETEEMLDFCR",
    "response#3": "GVGHMGVKLAKAMGHHVTVISSSDKKKEEAIDHLGADAYLVSSDATQMQEAADSLDYIIDTVPVFHPLEPYLSLLKLDGKLILMGVINTPLQFISPMVMLGRKAITGSFIGSMKETEEMLDFCR"
  },
  {
    "reference": "MAKYIKNVVVLLLIALFVLFLVNRLLIQPANSETISYSQFLALVDSGQVQSVMLHQHEAQGKKKDGNTFSVAIGDDAMVVPQLVKHHVAISYAEPSANANLLAAAGNFLLPMILLVVLLFFVVRQMQVGGSQALSFGRSKAKLLSENRPKVTFADVAGVEEAKQELAEIVEFLKYPKKFQALGARIPKGVLLLGPPGSGKTLLARAIAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSSPCIVFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFDANQGVILIAATNRPDVLDPALLRPGRFDRQVVVDRADVKGRAAILNVHAKNKPLGKDVSLDTLARRTPGFSGADLENLLNEAAILAARENKSVVEMRDCEEAIDRVIAGPERRSRLISQKEKEIVAYHESGHAIVGAMLPNANPVHKVTIISRGMALGLTMQLPVEDRYLMSRAEILDQITGLLGGRVSEELQFKEVTTGASNDFEKATQLARSMVTQYGMSRELGPIQYGHNHQQVFLGRDIADERNYSEEIASKIDSEVRSIIDECYNTAKQILQENWEKLERLVAALLEKETVEADEVLAIAKGEALPDKAGESLPAAASQGQEPATEPTRQSKPRPIPPNISPEPA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a high affinity for ATP.2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.3. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins4. The designed protein must have at least one AAA+ ATPase domain.5. The designed protein should have ATP hydrolysis activity to facilitate protein catabolic process. The designed protein sequence is ",
    "response#1": "LLVGPPGTGKTLLAKAVAGEAKVPFFSLTGSDFIELYVGVGASRVRDLFKEATKNAPCIIFIDEIDAIGRSRDSKYGGGNEEREQTLNQLLSEMDGFDTSKGILVLGATNRPEILDKALLRPGRFDRRIIVL",
    "response#2": "IPKGVLLFGPPGTGKTLLAKAVAGEAGVPFMTISGSDFVEMFVGVGAARVRDLFEQAKQSAPAIVFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVELDGFEGNSGVIVMAATNRPDILDPALLRPGRFDRQIVVDAPGLEFDNAVILFAD",
    "response#3": "IPKGVLLMGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKNAPSIVFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFGTNTGVIIIAATNRRDILDPALLRPGRFDRQIAVGYPLNFAEAKGISARDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFESA"
  },
  {
    "reference": "MKKILVIGSTNIDFVVNVVDFPVAGETVMSQSFQKMPGGKGANQAYACGKLGGDTAFVSVIGDDGLGDLAMKNLKDAGVNIDSVKTAPGTPTGMAQISIDHSGNNCIIVVPGANSACNQEFIEEIYDRIDACDILLIQMETPLAGVLKAIQRAKQAGKTVILNPAPVPEEPLPEELFNGLTYLTPNETELGKLTGMPVDTFEEVCAAARKLLELGVEHVIVTLGEKGAAFINRETEVLYETRKVEAVDTTAAGDTFNAAIAVFLSHAKTIEQAIRFANLASGISVTRQGAQPSVPSREEVEALAAEIG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The ATP, K(+), substrate binding site should be stable and able to withstand changes in environmental conditions.2. The ideal protein would be one that plays a critical role in lactose metabolic process, D-ribose catabolic process, D-tagatose 6-phosphate catabolic process through metal ion binding, ATP binding, tagatose-6-phosphate kinase activity, ribokinase activity within cytoplasm.3. The protein design should take into account the physiological conditions of the D-glyceraldehyde 3-phosphate and glycerone phosphate from D-tagatose 6- phosphate: step 1/2, Carbohydrate metabolism, D-tagatose 6-phosphate degradation pathway.4. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of ATP + D-tagatofuranose 6-phosphate = ADP + D-tagatofuranose   1,6-bisphosphate + H(+).5. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium; The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway..6. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "MKKICVIGSLNIDLVVKVDNMPKKGQTLIGSDFNEVPGGKGANQAVAMARLGGAVNMIGKVGADGFGVRLMEQLKNNNVNTNYISVEECATGVAMITVDKNADNSIVVAPGANFKVDKEYIDKSIEAINCSDIIVLQLETPLETIKYALKKSKELGKYTILNPAPAVKLEDELIANVDLLTPNETELEILSGVEIKSDEDIEKAAKTMIEKGVKELIVTLGSKGSLYINKDRSIFKKSYKVNAVDTTAAGDSYTGALAVAFAENKSIEEAMDFASRVGALTVMKEGAQSSLPENLDFFTKKAKIMY",
    "response#2": "MKKICVVGSLNMDLVVTVDRFPEPGETITGREFATYPGGKGGNQAVALGRLGADVLMIGKLGNDLFGEQYLNNLKENGVILDGVKVEEGVSSGIAVIEVDSTGENHIVIVPGANGKVDRAFIEENWHLMEERDIFLFQLEIPIDTVLYAVKKLKKAGKIIILDPAPARILPDDIFLNIDYITPNETEIETLTGIRIKDVDDLKEAGHWLVKKGVKNVIAKAGKKGAYLATSDDFVHVPGFTVEAVDTTAAGDSFNAGFAFALAQGKGIIESIQFANAVGAISTTAKGAQSAMELAFLSKFE",
    "response#3": "MKKICVVGSLNMDLVVTVDRFPEPGETITGREFATYPGGKGGNQAVALGRLGADVLMIGKLGNDLFGEQYLNNLKENGVILDGVKVEEGVSSGIAVIEVDSTGENHIVIVPGANGKVDRAFIEENWHLMEERDIFLFQLEIPIDTVLYAVKKLKKAGKIIILDPAPARILPDDIFLNIDYITPNETEIETLTGIRIKDVDDLKEAGHWLVKKGVKNVIAKAGKKGAYLATSDDFVHVPGFTVEAVDTTAAGDSFNAGFAFALAQGKGIIESIQFANAVGAISTTAKGAQSAMSHLDQLISS"
  },
  {
    "reference": "MLEPLYTADEMRAAEAGHDVEQLMARAGRAVAEEAMRRFPQARRFVAVCGSGANGGDGRIAVDVLRSAGRDAVVADDLAGADVIVDALFGTGFHGEPREDAARKIEAINAAGAPVVAVDLPSGVNASTGEVAGACVDATLSVTMHGRKVGLEVAPGRFHAGEVVVADIGLERRETELRLVTTAILEQVPRKRPDQNKYSAGTVLVVGGSRGLTGAPSLAAEAAFRADAGYVAVAVPDSTLPVFEQRLLEAVKLPCPEDEGKISPRAIEPIVEFAGKAGAVALGPGLGRGPGPKEVVARLLAELETPVVVDADALFELEPFERSAPTVLTPHEGELARLLGEESSWVAAHRVEAARRGAEKFGCVCLLKGADTLIAEPASGIWVCALGTPALATAGSGDVLTGVVSAFLAKGLGAGLAAAAAAAACQLASREIPQRGAVASDVIHALPAALN",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration2. The (6S)-NADPHX binding site should be stable and able to withstand changes in environmental conditions.3. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.4. A protein with metal ion binding, NADPHX epimerase activity, ATP binding, NADHX epimerase activity functions is required to enable nicotinamide nucleotide metabolic process.5. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "LLTWQHQRLPEIPSNAYKHQRGKVVVVGGDLGMGGAVIMAAEAALRCGAGLVSVVSREEHRTALLARIPEAMFIDPESIALQQALDTADCIVLGPGLGREAWGAKLFATAAAAAVPKVIDADGLYWLGNAEQNPSNDLFLTPHSGEAANLLGVDVAAIEADRLTCGLQLARNYGCSLVLKGPGSVVVTDKGIQICAHGGPAMATAGMGDVLSGVCAGLLAPMYAGNTSDQAHDQFAQAVALHSASADLVAATLGSHSVMATDVISGLPNLLSGG",
    "response#2": "MRDADSFTIASKSIFSADLMEEASHAFVKIFTEEFPDRSLRVSVYCGTGNNGGDGLAISRLLMQAGYHDIKVMIARFSANSTDDFEINFRRLREFQNIRIQELRSAVECGAETSDLIIDALLGSGLNKPLSGEWEKLVTWLNSLHKTIVSVDIPTGLPAEEGAGSGHAILHARLAISFQRPKLSFLLPGSAPYLDRFRVADIGLDDDFIGPGDVSIVVKPRRPEAHKGDFGRLLIVGGSETFSGAPALAALAALRTGVDLVYIAAPRETAYSISSMSPDLITLKLEGDHLSMRNMPIIRNYMEKVTAVAVGPGLGLHKETQEAVSEIIRIAEERKMPILLDADGLKAFAGFKRRLETPAVLTPHLGEYAILTSEKPPDNLEERGNHVKKTAENLNATILLKSHVDIISDGFKTKFNFTGNPGMTVGGTGDVLTGIVAAFLAQGVDPFEAAVAGAFINGAAGDFVKAEKGYHMVATDLLDWIPKVIDDPMGIIGGNTGMNGALQLAGASALYSGCGKVSMIPLDQSFKADMSMPELMTKSLKNVPKNINIFSALAVGVGFDTTDDSQQILESIIDNLTQLAIFDADALNIIVTNQQIREKFINLENKIITPHPAEAARLLDCTTKEIQEDRFTAIRTLAKKYNATVVLKGAGSLICKDNKIYINTTGNQGMAVAGQGDVLSGIIGAFLAQGLNTLSASRLAVYVHGLAGDNVAKKLQ",
    "response#3": "MAVIDRNAQYFDKSEMDLMEKAGKKVAEEIEFGESASVFCGEGNNGGDGFVVARYLKEKGYEVFVYLVGDSSSLRTEAAKKNFEELKKLDVTIFEEKDPGFYDNLEISSDVVVDAILGLGIKGKPREPQKSAIKAINNSTAKKISVDIPSGINPDTGEGKGIKPDKTITFHEKKKGNKNAKTVEIGIPQKAKAGPGHLYYLNQRKKTSHKGENGTILILGGGEFTGAPAISAMSALRSGVDLPIIHTKNKVKDIIAGFSPNLIVKSHKNYKKAQKNLKNNIKKFDSVLIGPGIGREKKDINALIKLIPKINKPTILDADALHAATKDIDMINNKIITPHESEFKKLFNKKGTKENVKKMAKKHSCIIVKKSNIDIISNGQKTKLNESGTPAMTVGGTGDALAGAIAANVAKNKNNLFETTVATTFLIGKAGELAEKEYGNGLTATDLIEKIPKAKEKLNF"
  },
  {
    "reference": "MEPVDPRLEPWKHPGSQPRTPCTNCYCKRCCFHCQVCFITKGLGISYGRKKRRQRRRSPENSQTHQDSLSKQPTSQFRGDPTGPKESTKKVESKAETDQFD",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Target a Basic and acidic residues, Polar residues compositional bias in the protein's composition for improved properties.2. The protein must exhibit the following characteristics: Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication; Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to promote processive transcription elongation by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts; In the absence of Tat, the RNA Pol II generates short or non-processive transcripts that terminate at approximately 60 bp from the initiation site; Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation; In the cytoplasm, Tat is thought to act as a translational activator of HIV-1 mRNAs.; The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive.3. Incorporating a Nuclear localization signal, RNA-binding (TAR), and protein transduction, Cell attachment site motif is critical for the successful design of the protein.4. The designed protein should have a binding site that is specific to the target Zn(2+) ligand. The designed protein sequence is ",
    "response#1": "MEPVDPNLEPWNHPGSQPKTPCTKCYCKKCCYHCPVCFLNKGLGISYGRKKRGQRRRTPQGNKDHQNPVPKQPLSQTQGDSTGPEESKKKVESKAETDRFD",
    "response#2": "MKVKETRKNYQHLWKWGTMLLGMLMICSAAEQQWXVVXTAIXNXSXHXRXGXEKXXRXXGAXAXXXEXXXRXTXAXRXDVXXXYPXVXXXGXNXRXRXRXXXVPXEXXXXXXXXBXNXXRXXXAXXXPXXXAXXXXXCBNXRXRXHXXXAPXAXXXXXXXXXXBNXXXRXXXAXAXXXXXAXIXMMXXX",
    "response#3": "MEPVDPRLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFTRKGLGISYGRKKRRQRRRPPQGSENHQASLSEQPASQPRGDPTGPKESKKKVERETETDPVD"
  },
  {
    "reference": "MRATRPARPPRTPLSEVARRLGIPFPAATAGNPEPEVTGVTLASSRVLPGDLYAALPGARVHGATYVADAVARGAVAVLTDAEGAARLADLPVPVLAVEQPRRLLGALAADLYGAPAAALRLIAVTGTQGKTTTTRLADQAFEAAGITAGVIGTVGTRVAGEEIRTSLTTPEAPDLHGLFAMMRERGVEACAMEVSSHALVMGRVDGVVFDVAVFVNLGRDHLDFHADVEDYFQAKASLFTPARARLGLVNIDDPYGRRLAAEAGIEIRTFSATGAPADWRALDVRLAPDGSEFTVVGPDDEWASTWVPIAGDFNVANALAAIAAMTEAGFPLADVAAGMAAADGVPGRLEKVEAGQDFHVVVDYAHKPDAVEAALTTLRPLTEGRLIVVIGAGGDRDTGKRPVMGEIAARLADVVVVTDDNPRSEDPAAIRAAVLAGTTGGGAEVREVGDRRLAIRTALALADPGDIVLVAGKGHERGQEIQGEVHPFDDREVVREELAGSTGEALG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The meso-2,6-diaminoheptanedioate binding site should be located in a region of the protein that is accessible to the ligand.2. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.3. The designed protein should contain one or more Mur ligase N-terminal catalytic domains that are essential for its function.4. The designed protein should have ATP binding to facilitate regulation of cell shape.5. A protein that can modulate the overall flux of metabolites through the peptidoglycan biosynthesis pathway, enhancing or suppressing its activity as needed.6. The protein should have a low Km value for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "MTIQHLQLDSRKIQPGDTFVAVAGHAVDGRQFIADALARGANLVLAQADEQHPHGTVSDIDSKPIIYLASLDEHLSALAGRFYGFPSRRLSVIGVTGTNGKTTTTYLIERILEDYGVTGTNGKTTVATVLFQLFEQLGESCGLISTVENKIGVRVIPATHTTPDAISLQTLFAQMRDAGCTYVFMEVSSHAVHQHRIGGVQFDGGIFTNITHDHLDYHKTFDEYISVKKSFFDHLPKTAFALTNVDDKRGAVMLQNTKALKQAYALRVPADFKGKVLENNLTGLLMQVNNQEVYFRMTGLFNAYNLLAAYGAAVTAGLSAEEALRGISSCEGVPGRCERAVELSNGATAYVDYAHTPDALEHVLASLRPHTTGRLIVVFGAGGDRDRGKRPLMGDVASKMADIAIITDDNPRSEDPTLIRAEIKAACPDAIEIADRRAAIAAGLDALQAGDILVVAGKGHETYQIVGDKKLHFDDREELRRALG",
    "response#2": "MKLSELLKGIPYTVVKGDLDVEVSNLVLDSRKSTAGAMFFALKGAAVDGHTYIETAIEKGAVAIVCSELEDSREVNCVVTDSRRIIPGALFFALPGRRADGHAFLDDAIARGAAAIVSGQPVSGLPAVAAAQVADPRRVLAEVARRFFESPSTKMKIVGVTGTNGKTTVSTLLRHLLQEGKKTGVLGTLRYYWGDSVIEAELTTPDALTLYRLLKKMVDEGCEYCVMEVSSHALELKRVWGLEFEAAIFTNLSQDHLDFHKTLEEYWRAKASLFSQEGKQPGLAVINRDDEYGKRMKEAGACLFTYGIDKGADVRAENIVLSPEGTSFSLCYRGKEYPVSLPLVGRYNLYNVLAVVGASAILGIPEESIKEGLSRVPAVPGRFETMQSPVEKILGIVDYAHTPDALQNVLSTIRQFGQQANIITVVGCGGDRDKTKRPEMARVACDLSTTTILTADNPRTENPETILDEMVAGLTPGQYRKMIRISDRKEAIKTACNLARANDVILVAGKGHETYQIIGKEKHHFDDREEARKALGEV",
    "response#3": "MKLRDLISPLPHQYPAGDSSLQADSRKVKPGDLFICLPGFTVDGHDYAPQAAAQGAAALVVERKLAVPDELPIIRVDDSRKAFGRAAANWFGHPADSMKLVGVTGTNGKTTVSTLLRHLLQEGQQCGLIGVTGTNGKTTTTYLIERILEDYGHKTGLIGTIEIIIGSERHLAENTTPESVDLQRYMAEMVKAGCDSVVMEVSSQGLMLHRTAGFVFDYGIFTNLEPDHIGPNEHADFADYLRCKSLLFRQCRVGILNGDDPHTEDILRSHTCRLETYGFSPLADLRAVDVRPVAVEGRAGVSFVLHGREGALDIDSRLTGEFNVYNALAAIATALAMDVPPALIKAALTSAAARGRCERAVELSNGATAYVDYAHTPDALEHVLASLRPHTTGRLIVVFGAGGDRDRGKRPLMGDVASKMADIAIITDDNPRSEDPTLIRAEIKAACPDAIEIADRRAAIAAGLDALQAGDILVVAGKGHETYQILKDKTIHFDEREIVAEIIASGGRG"
  },
  {
    "reference": "MEDSEFLAYVELLDEVEQGSVRAKASSVSLHAERTWMEKMKVEDLNVCEPASPAPEAPATSLLNDLKYSPSEEEEVTYTVINQFQQKFGAAILHIKKQNVLSVAAEGANVCRHGKLCWLQVATNCRVYLFDIFLLGSRAFHNGLQMILEDKRILKVIHDCRWLSDCLSHQYGILLNNVFDTQVADVLQFSMETGGYLPNCITTLQESLIKHLQVAPKYLSFLEKRQKLIQENPEVWFIRPVSPSLLKILALEATYLLPLRLALLDEMMSDLTTLVDGYLNTYREGSADRLGGTEPTCMELPEELLQLKDFQKQRREKAAREYRVNAQGLLIRTVLQPKKLVTETAGKEEKVKGFLFGKNFRIDKAPSFTSQDFHGDVNLLKEESLNKQATNPQHLPPTEEGETSEDSSNKLICTKSKGSEDQRITQKEHFMTPKHEFQASLSLKEETEQLLMVENKEDLKCTKQAVSMSSFPQETRVSPSDTFYPIRKTVVSTLPPCPALEKIDSWISPFLNLP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposable elements, preventing their mobilization, which is essential for the germline integrity ; EXD1 is an inactive exonuclease .2. The protein should be optimized for stability and solubility when expressed in P granule where it will be involved in meiotic cell cycle, RNA-mediated gene silencing.3. The designed protein should contain one or more 3'-5' exonuclease domains that are essential for its function.4. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "MVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISKPQALMTVATKIALQMNCKMGGELWSVEIPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYEPKLSVVVVKKRISSRFFARIDGKIANPPPGTVIDTEVTVC",
    "response#2": "MVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISKPQALMTVATKIALQMNCKMGGELWSVEIPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYEPKLSVVVVKKRISSRFFARIDGKIANPPPGTVIDTEVTVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISKPQALMTVATKIALQMNCKMGGELWSVEIPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYEPKLSVVVVKKRISSRFFARIDGKIANPPPGTVIDTEVVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISKPQALMTVATKIALQMNCKMGGELWSVEIPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYEPKLSVVVVKKRISSRFFARIDGKIANPPPGTVIDTEVMVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISKPQALMTVATKIALQMNCKMGGELWSVEIPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYEPKLSVVVVKKRISSRFFARIDGKIANPPPGTVIDTEVT",
    "response#3": "MVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISKPQALMTVATKIALQMNCKMGGELWSVEIPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYEPKLSVVVVKKRISSRFFARIDGKIANPPPGTVIDTEVTVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISKPQALMTVATKIALQMNCKMGGELWSVEIPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYEPKLSVVVVKKRISSRFFARIDGKIANPPPGTVIDTEVTTQC"
  },
  {
    "reference": "MAKLNMAQQLASTLEQAGIKRIWGLTGDSLNGLTDALRSMDSIEWMHVRHEEVAAFAAGAEAAVTGELTVCAGSCGPGNLHLINGLFDCHRNRVPVLAIAAQIPSSEIGLNYFQETHPQELFKECSHFIELVSNPAQMPQVLHRAMRSAILNRGVAVVVIPGDVSLLEVEQKPAAWPTLQQPRILPTDVDLQRLTELLQDSHKVTLLCGSGCAGAHDQVVALADALGAPVVHALRGKEHVEWDNPFDVGMTGLIGFSSGYHAMLGCDTLVMLGTDFPYRQFFPSDAKIVQVDRDPQALGRRATLSLGIAADVGETLTALLPRLTRKTDRRFLDDALKAYDKARQGLDDLAVPSKGDRPIHPQYVTRLLSELATEDAIFTADVGSPTVWAARYLKMNGQRRLVGSFNHGSMANAMPQAMGAQAAFPGRQVISMSGDGGFSMLMGDFISLAQLKLPVKVVVFNNASLGFVAMEMKAAGYLDTGTDLHNPDFAAMSNAMGILGIRVERSEDLEQALRRALAHDGPVLVDVVTATQELVMPPAIKLEQAKGFSLYMLKAVMSGRGDEVLDLAKTNLLR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that can perform lipid binding, pyruvate dehydrogenase (quinone) activity, magnesium ion binding in plasma membrane to support pyruvate catabolic process.2. The protein must exhibit the following characteristics: A peripheral cell membrane enzyme that catalyzes the oxidative decarboxylation of pyruvate to form acetate and CO(2); It channels electrons from the cytoplasm to the respiratory chain at the cell membrane via ubiquinone..3. The presence of Thiamine pyrophosphate enzyme TPP-binding domains is critical for the interaction of the designed protein with its intended targets.4. The protein should be able to bind Mg(2+), FAD, thiamine diphosphate ligand in a variety of conditions5. The protein should be designed to have high stability and activity towards a ubiquinone + H2O + pyruvate = a ubiquinol + acetate + CO2. The designed protein sequence is ",
    "response#1": "AALARMLNDCSRVTLLCGGGCAGAHDEVVALAAALKAPVVHALRGKEHVEYDNPYDVGMTGLIGFSSGYHAMKECETLLMLGTDFPYRQFYPTEARIAQVDLRGEVIGRRCRVDVGVVGDVKLTLARIAERHPGAVAADALAALLNAGKRVTLLCGRGCAGAHDELMALAGALKSPIVHALGGKEYIEYDNPFDVGMTGLIGFSSGYYAMEDCDTLLMLGTDFPYRQFYPEDATIAQVDLRPANIGRRCRVDLGVVGDVKATLQTLVVAPDHPARDAARERIDRLADLLNHSGGVTLLCGSGCKDAHDDVVAFADTLGAPTVHALRGKQYVEWSNPYDVGMTGLIGFSSGYHAMMSCDTLVMLGTDFPYRNFYPPHAKIVQIDSDPAALGKRAPLALGLVGDVRETLRTVAENIVSVLRHHGVQRVYGLPGDSLNGFTDAIRRDGDIRWMHVRHEEAAAFAASAEAELTGELTVCAGSCGPGNLHLINGLYDANRSRVPVLAIAAHIPTAEIGSTYFQETHPQELFRECSVYVEYVADPVQMPRLLEIAMREAIEKRGVAVLVIPGDHPGRDAARDAEAAAVLNGAENVTILAGAGVAGAHDEVVALAAKLQAPVVHALRGKEHIEWENPYDVGMTGLLGFASGYRAMERCDALLMLGTDFPYRQFYPAKARIVQVDIRGEQLGRRTPIDVGLVGGVRETAAALVATGGRPGTDAARVGADALAALLNAGKRVTLLCGRGCAGAHDELMALAGALKSPIVHALGGKEYIEYDNPFDVGMTGLIGFSSGYYAMEDCDTLLMLGTDFPYRQFYPEDATIAQVDLRPANIGRRCRVDLGVVGDVKATLQT",
    "response#2": "TAADYLVGALAQAGVQRIFGVVGDSLNGFTDALRRDGSIEWVHMRHEESAAFAAGAEAHLTGQLAVCCGSCGPGNLHLINGLFDCQRSGLPVLAIAAHIPSTEIGIDYFQATHPEALFKDCSHYVELVSNPGQLPQILGRAIRTAVARKGVAVVVMPGDVGGTLGEEWLAAGAALARMLNDCSRVTLLCGGGCAGAHDEVVALAAALKAPVVHALRGKEHVEYDNPYDVGMTGLIGFSSGYHAMKECETLLMLGTDFPYRQFYPTEARIAQVDLRGEVIGRRCRVDVGVVGDVKLTLAGLLGEPPDEPARLADRAALARMLNDCSRVTLLCGGGCAGAHDEVVALAAALKAPVVHALRGKEHVEYDNPYDVGMTGLIGFSSGYHAMKECETLLMLGTDFPYRQFYPTEARIAQVDLRGEVIGRRCRVDVGVVGDVKLTLAPLLGAPAGERLDALIDRLADLLNHSGGVTLLCGSGCKDAHDDVVAFADTLGAPTVHALRGKQYVEWSNPYDVGMTGLIGFSSGYHAMMSCDTLVMLGTDFPYRNFYPPHAKIVQIDSDPAALGKRAPLALGLVGDVRETLRALAPGR",
    "response#3": "TIADYLAKTLAAAGVERIWGVTGDSLNGLAFSLEELGSIRWMHTRHEESAAFAAGADAASTGRLAVCAGSCGPGNLHLINGLFDCHRNHQPVLAIAAHIPSSEIGLGYFQETHPQELFRECSHFAELVTNPEQFPRVLERAMRTAIEEHGVAVIVLPGDVALKLERAATLLNAASKVTILAGAGAEGAHDEVVALADRLAAPVVHALRGKEFVEYDNPFDVGMTGLLGFASGYRAMEGADALLILGSDFPYPQFYPADATTIQVDIRGENLGRRHPLDLGLVGDVGATAAAARRVLEGRPIDRLADLLNHSGGVTLLCGSGCKDAHDDVVAFADTLGAPTVHALRGKQYVEWSNPYDVGMTGLIGFSSGYHAMMSCDTLVMLGTDFPYRNFYPPHAKIVQIDSDPAALGKRAPLALGLVGDVRETLRDRLAALLDGAEAVTLLCGAGCAGAHDEVVALADLLAAPVVHALRGKEHVEWDNPFDVGMTGLIGFSSGYYAMESCDALLMLGTDFPYRQFYPTKAVVAQIDLRPEVLGRRTRLDMALVGDVGPTLRAIDRLADLLNHSGGVTLLCGSGCKDAHDDVVAFADTLGAPTVHALRGKQYVEWSNPYDVGMTGLIGFSSGYHAMMSCDTLVMLGTDFPYRNFYPPHAKIVQIDSDPAALGKRAPLALGLVGDVRETLRDRLAALLDGAEAVTLLCGAGCAGAHDEVVALADLLAAPVVHALRGKEHVEWDNPFDVGMTGLIGFSSGYYAMESCDALLMLGTDFPYRQFYPTKAVVAQIDLRPEVLGRRTRLDMALVGDVGPTLRA"
  },
  {
    "reference": "MAERPVLLLLFLEQTTVVVLEKSKGRRDEKRWSRTRLSGGDRMNRNNKNNSPRPTKKSKKSAGEEKASHSGGSVHKKNLYFVSFPVIILFNVLRTLIYQLYVIFGYIYSSTSRIVYRPRAGSPVEVVIPRDEQDDDAAYLQPCGPEEYSSMALPRQPTGPGPGDPLLAKQKSHHRRAFEYISKALKFDEENEGQKEMAVELYKKGIAELEKGIAIECAGKGEVWERAQRLQGKMKTNLEMAKDRLDFLEQIVMLTHLAVTDDPEAVAATKKPLKRPTCFPRRPSAPPQLLLAGATRNPEVKSPRAPTLRPTPQRSQPTTPAEASGRRLTAAGRKNIPGMAARSSTLPRNLGSRQPVYTNGSGSNSGAAKHKPYSTPPPIKRQLSGPSHSPGRRPSGGNGASGRVAAARKTSLKGVDPKLAQLILDEILEGGPPVEWDDIAGQESAKQALQEMVVLPALRPELFTGLRAPARGLLLFGPPGNGKTLLARAVAAECSATFFSISAASLTSKFVGEGEKLVRALFAVARELQPSIIFVDEVDSLLSERREGEHEASRRLKTEFLVEFDGLPSSTEDRVLVMAATNRPQELDEAALRRFSKRVYVTLPGPDTRMALLGRLLSKQGSPLSEKDLAQLARLTEGYSGSDLTALAKDAALGPIRELDPDQVRCLDPKHMRNIKLQDFTESLKRIRKSVSPLSLVQYEKWNSEYGDVSL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should contain Cytoplasmic topological domains.2. The ideal protein would be one that plays a critical role in microtubule severing, positive regulation of microtubule depolymerization through microtubule binding, ATP binding, microtubule severing ATPase activity within centrosome.3. The designed protein must have at least one MIT domain.4. The protein design should emphasize Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "PPKGLLLFGPPGNGKTMLAKAVACESNSTFLNISASSLTSKYVGEGEKLVRALFAVARELQPAIIFIDEVDSLLNERKENEHEASRRLKTEFLVEFDGLRSDGDDRMLVMGATNRPQELDDAALRRFTKRVYVTLPY",
    "response#2": "PAKGLLLFGPPGNGKTLLARAVATECSAKFFNISAASLTSKYVGYGEKLVRALFSVAREMQPSIIFIDEVDSLLSERNSNEHEASRRLKTEFLIEFDGLPGNPDGDRIMVLAATNRPQELDEAALRRFTKRVYVSLPT",
    "response#3": "PSRGLLLFGPPGNGKTMLARAVATACNATFFSISAASLTSKYVGQGEKLVRALFAMARELQPSIIFIDEVDSVLSERKEGEHEASRRLKTEFLLEFDGLHSNSEHRLLVMGATNRPQELDEAVLRRFSKRIYVTLPH"
  },
  {
    "reference": "MAKILLGVSSSIAIYRACELVRELVKSGHQVRVVMSPFSERFISRLTFQALSGNRAYVDWEDDPLLHINLPRWSDLFLVAPCSINTLSKIALGIGDNLLTTCALSHKGTLMLAPAGNVEMYKNPAVQENIKRLKDRGVIIVDPEEGKLLCEEEGQGRLASLERLLDWVEYALRPKLLEGKRVLITAGSTREFIDSVRFISNLSSGLMGFSLARVFRWYGAQVKVVAGFTTAEEPPEVEVIRVVSAQDMFERVMHLKDWADIVVMNAAVADYRPSEVYGGKLKKRERFTLELVKNPDILAELGRSEGDKFLVGFALEERERLEGNALKKLQEKNLDLIVANPLESMGSETYEGRLFFRDGRRLDIKAGRKLEASELIVKELIKTLLKAP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Proton donor of the protein must be engineered to enable effective catalysis of H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.2. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.3. The designed protein should contain one or more Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains that are essential for its function.4. A protein with metal ion binding, FMN binding functions is required to enable coenzyme A biosynthetic process, pantothenate catabolic process. The designed protein sequence is ",
    "response#1": "KRIIVGITGGIAAYKAADLVSQLVKAGAEVRVIMTPSAQKFITPLTLQTLSRQPVGLDMFAEPKAWEVQHIAWADWAQLVIVAPATANTIGKLAHGLADNLLTAVLMATRAPILLAPAMNVHMWENPLVQANLEKLQAVGVRIVEPESGRLACGYEGKGRMAAVNTILLSGKRVLISAGGTREFLDPVRFIGNPSTGKMGIALAQAALDRGADVTLVHAPLGILEPLPGNLEAIAVVSAAEMHEAMLQALPQADWIIMAAAVADVKPSHTSLEKLPKSALPDSLPLVPVPDIVTELARHKQPHQRLIGFAAQTGDIVTPAKDKLERKKLDAIAANAIDQPNSGFGSDMNQAVLDFVCQA",
    "response#2": "KKIVLGISGGIAAYKSPELVRRLRDRGAEVRVVMTHAAKAFITPLTLQAVSGYPVSDDLLDPAAEAAMGHIELAKWADLVIMAPATADLLARVATGMANDLLTTACLATAAPIAAVPAMNQQMYRAAATQANLQTLASRGMLLWGPDSGSQACGDVGPGRMLDPLEIVALAHDVGLGVCGSIAAYKAVELVRLLRAEGAEVVVMLTPAAAQFVGPLTFAALSGHAVETDVLGLLPDQRIGHIVVADAADAIVVAPATARWLGAMANGIASDTVVAACLATAAPVVFAPAMDGDMWTHPATQSNVGRLERDFGYRMVPPQIGSLASGQSGIGRLAELPLIVE",
    "response#3": "KRILLIVSGGIAAYKVLDLIRRLRERGAAVTPVMTRAAAEFVTPLSLSALAGEKVFRDLFDLTDEAEMGHIQLSRSADLVVVAPATADLMARMAGGHADDLASTLLLATDTPVLVAPAMNVRMWQHPATRRNLATLRGDGVRVVGPNEGDMACGEFGPGRMAEVPEILAAIEATLG"
  },
  {
    "reference": "MRLNACSLAILAALLLCAASSAAAGSEQQRLQPGGLAGGGARARAAGAAGLALQRRLLDDDDHEHERDRDDDHPRGWSRKGASDTAAAAGSVSGSITEDSVGTAAGANATAASAAVKPVVKRPTPAVVSKGAMPFSSWMSDVMPNDAWLMMSFVVAWFCAPGVLLLLWPFVASHRTAEPLKPAAAGCAEAGGPTAAAIMGIQGLTKLLADAAPGSMKENKFENYFGRKIAVDASMHIYSFLVVVGRQGDQLLTSEAGDVTSHLQGMFFRTVRMLEAGMKPVFVFEGKPPELKRAELAKRSTKKEEATTELEAAKEAGNQEDVEKYSKRAVKVTRQHNEECKQLLRLMGVPVVEAPGEAEAQCAQLCKENLVYGISTEDMDSLTFGTPRLIRHLMAPASQKLDAMEFDHAKVLEELGLSRDEFIDVCILCGCDYTPKISGIGPTRALALIKKHGSIEKALESLDPSKYTIPEPFPYQEARQLFKEPDVLRGEAIPSIKWSAPDKDGLIQFLVHEKSFNEDRVRKALDRIAAAKGKASQGRLESFFGPAKIVSSTSGKRKEPEVAKGKGGKAGAAKKGKLGAMGKKNSRRRHPPPPALTPCAAPPSCSRCWPSWRPQALAATTATTTIATMGTTATTTTTTEPSHPPSHTGASCGTETAALELAYEEFDSDAFEAGVGRFSHGLSDIADVRLRCIAVERNPARRRSLLAAAPVLVTFELKATDATAVAQALTDAARDGTLEELLALLGGQLSGYTILDGQSMPWAAAEAP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a high specificity for DNA binding, with minimal off-target effects.2. For general function, the protein need meet that Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site- terminated flap; Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions; It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation; Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity3. The designed protein must have at least one XPG N-terminal domain.4. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.5. The protein should have Helical domains to ensure proper insertion into the membrane.6. A protein that can perform DNA binding, 5'-flap endonuclease activity in mitochondrion, nucleolus, membrane to support DNA replication, removal of RNA primer. The designed protein sequence is ",
    "response#1": "MGIKGLMKFLQENAPKAVREIDSQQAYTGRVVAIDASMCLYQFLIMIRENRSGSYNNLTNEDGHVTSHIIGMLTRTIKLMESGIKPVYVFDGKPPELKLQELDARRAKRDEGRIIAIDASMSLYQFIIAIRDSDQYGNLTNESGETTSHISGLMSRSIKLMENGLKPIYVFDGAPPELKGSELEKRGEKRQKAEELLQKAKEEGNLEEIKKQSGRTVRVTKKQNEEAKKLLTLMGIPVVEAPCEAESQCAFLTKYNIAHATATEDADALVFGTKILIRNLNANASSNQNKNKNSSKRGYILTEINLQQVLEGLNLTMNQFIDFCILCGCDYCDTIKGIGSKTAYNLIKEYNCIENIIQNIDKNKYQIPANFRFEEARNSFLNPKVLTKEEIKIDWNEPKIGIPYVEAPCEAEAQCAELARGGKVFAAASEDMDTLCYEPPYLLRHLTFAEARKMPIDEIQYATALEGLGLDKSTFIDLCILLGCLGIPVVEAPCEAESQCAFLTKYNLAHATATEDADALVFGTKILIRNLNANASSSANQNKNKNSSKRGYILTEINLEQVLKGLNLNMNEFIDFCILCGCKMDQFIDFCILCGCDYCDTIKGIGSKTAYNLIKEHGNVQGRVVAIDASMCLYQFLIMIRENRSGSYNNLTNEDGHVTSHIIGMLTRTIKLMESGIKPVYVFDGKPPELKLQELDARRAKRDEATASLQAAIEAGDSEQVLKSTKATVRVTREQNEETKQLLRLMGVPVVDAPSEAEATCAALCRDGKVYAAATEDADCLTFGTKILVRNLMAAESQKKQILEVNLERALEQLNVSMDQFIDFCILSGCDYCDTLKGVGPSTAIKLLVQHGSLEKVLEELGPEKVPKNFRYQAARDFFRECEAVDTAKVDFKFEDPDCEGLT",
    "response#2": "MGIKGLTKFIADAAPNAIKEIKIENLMGRIVAIDASMSLYQFIIAIRDSEQYGNLMNESGETTSHISGLMSRSIKLMENGLKPVYVFDGAPPDLKGSELEKRGEKRQKRAAAAAAAAATTTTATSSTTTTTSTTSSTVSNSSNNSSNNSLDDSADGSDAEEDVPQPLPPHEIRIRNIPPRLPHRPPSGVPVIEAPGEAEAQCAALAKAGLVWATATEDMDALTFQTPHLLKRMTFSAKQQAPIMQISHDKLLHGLGITQEQFVDLCILCGCGRVKKFELFGDMVQCSITVEGIPVVEAPCEAESQCAFLTKYNLAHATATEDADALVFGTKILIRNLNANASSASQNKNKNSSKRGYILTEINLEQVLKGLNLSMDQFIDFCILCGCGRIQRFELFGSHAMCSIGRVVAIDASMSLYQFIIAIRDSEQYGNLTNELGETTSHISGLMSRSIKLMENGLKPIYVFDGAPPELKGSELEKRGEKRQKAEELLKKAKEEGNIEEIKKQSGRTVRVTKKQNEEAKKLLTLMGIPVVEAPCEAESQCAFLTKYNLAHATATEDADALVFGTKILIRNLNANASSSANQNKNKNSSKRGYILTEINLEQVLKGLNLNMNEFIDFCILCGCDYCDTIKGIGSKTAYNLIKEYNCIEKIIENIDKNKYQVPANFRFVEARDSFINPKVLSKEEVKIDWCEPKIEELKNFLIGIPVVEAPCEAESQCAFLTKYNIAHATATEDADALVFGTKILIRNLNANASSNQNKNKNSSKRGYILTEINLQQVLEGLNLTMNQFIDFCILCGC",
    "response#3": "MGIKGLTRLLGDNAPRALREQKFESYFGRTVAIDASMHIYQSMVVVGRSGDAMLTDADGQVTSHLLGMFYRTLRMLEAGIKPIYVFEGKPPSMKKGELAARREKREEEAAAKAQAEEAAKAKAEEESKAKAAAEEKAKAKAEAEAKAKAKAEAEAKAKAKAEAEAKAKAKATAEAKAKAKAAAEAKAKAEAAAAAEAAAAELEAEEEESKPTPKPKRKAPAAEKSPVQKAQPPKKAAAPVKKPAAKPAAAAKPAAKKPAPKPQAAAKPAASKPQAAAKPAAKPAAAKPAAKPAAAPAASKPAKKAAPKK"
  },
  {
    "reference": "MAQSASSSAAGCLAVVLAAGEGTRMRSNRPKVLHEIAGASMLSHVLRAVGAAGADRLAVVIGPDRADVECAARAVAADAEIVVQGDRLGTAHAVLRARAALERGPEAVIIAFADTPLVRPETFARLLAPLREGAAVSVLGFEAAVPTGYGRLVREGGQLVAIREERDGSDEERAITLCNAGLMGLRGDVALALLDQVQNHNAKGEYYLTDVVEIARAAGHSVAVVTAPEEEVQGVNDRAQLAGAERSIQERLRRAAMANGATLVAPETVFLAHDTRIGRDVVIEPNVIFGPGVVVEDGAVIHAFSHLEGARVGPKASVGPFARLRPGTVLGPKAKVGNFVETKNADVGAGAKVNHLTYIGDATIGADANIGAGTITCNYDGFFKYRTEIGAGAFVGSNSALIAPVRIGEGAYVGSGSVITDDVPADALAVARGRQAVKDGWAAAFRADAQARKAKT",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc); The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.2. The designed protein should be involved in lipid A biosynthetic process, regulation of cell shape, cell wall organization by localizing to cytoplasm and exhibiting UDP-N-acetylglucosamine diphosphorylase activity.3. The designed protein should contain one or more MobA-like NTP transferase domains that are essential for its function.4. Ideal protein should have a binding affinity for key enzymes in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "VVILAAGKGTRMHSALPKVLHQIGGRPMLLRVLDAAVSLSPDAIHVVVGVGAEQTIAIAEEAYPSAGINWVTQKEQLGTGHAVREALPGLKQSDADNSVLILFGDVPLITRSTLQRVIDHCGPEQVSLLYGEPPGRYGRLLREAGGDVVRIVEAKDATEAERAINLCNSGVLAAPAKLLFSLLEAVGNDNAAGEYYLTDIVGIARGKGLSAGVVRCAEAETLGVNSRVELAGAEAIVQDNLRRAAMDGGATLIDPASVWFSWDTKLGRDVVVWPHVVFGPGVVVGDNVTIKGFCHFEGCTIANGADVGPYARLRPGAEIGARAHIGNFVEVKKSVLEDGAKVNHLSYIGDAHVGAGANVGAGTITCNYDGFTKSRTQIGAGAFIGSNSSLVAPVSVGPGALVGAGSVITKDVSEGALAVGRGSQENRPGWAAKLRAALAARRAAKAAAAG",
    "response#2": "VIVLAAGAGTRMRSRTPKILHPLGGQSMVAHALAAARGVAPRDLVVVVRHERDLVADHVAGLDDSALLVDQDDVPGTGRAVQVALDALDQRAGAIEGTVVVSYADVPLLEATTLEALVGAHEQQGNAVTVLTAELADPNGYGRILRDFDGTVTGIVEHKDASPEQLAIREVNTGILAAPVEQLRRWLSAVRNANVQGEYYLTDVIAMAVADGVRVAAVAPERVFEVEGINDRVQLARLERAHQVGVARRVLDRLMRAGVTLPDPAATYIDAGVSIGADSLVHPGAHLRGTTKLGRNCIVEPGVVVSDCRIGDGVHLKVGSVLEEAEVGPGCVIGPMAHLRPGTQLAGDNKIGNFVETKKVRLDRGSQASHLTYLGDAEVGKDVNFGCGTITCNYDGRDKHVTVVEDDVFVGSDVQLVAPVRIGRNALLAAGSTITKDVPADALALSRSEQRVIEGWRRPVKTKK",
    "response#3": "MENGVTLPAPETVHFAFDTAIGRDTVIEQNVVFGPGVTIETGAHIRAFSHLEGCHVSRGAVVGPYARLRPGAELAEDTRIGNFVEVKNATIDEGAKVNHLSYIGDAHVGSGSNIGAGTITCNYDGVGKHHTEIGENVFIGSNTMLVAPVTVGNGALTGSGSVITKDVPADALAIARSQATLLGKKAIILAAGEGKRMKSKHSKVSHKICGKPMVQYVVNSVKGAMIDDVAVVVGHRAEEVKGCIKGDVTFVLQEKQAGTGHAVMCCEQFLEGKKGTTLVLYGDTPLISKDTIKDLVAFHNENNFSSTVLTANFKDPAGYGRIVRDTSGNIQRIIEDKDATEKEREIKEINSGIYCFDTEELIDALKQLHNDNSQGEYYLTDTLGIIKRKGLKVGAFLVKDYSETLGINDRVQLAEANAIMRKRINEFHMRNGVTLLDPARFDLRGELIHGRDVVLDANVIIEGKVELGDRVRIGAGCVIKNSVIGDDCEISPYSVVEDAVLEQKCTIGPFARLRPGAELAEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGAGVNIGAGTITCNYDGANKHKTIIGDDVFVGSDTQLVAPVSVASGATIGAGTTVTRDIAQNELVLSRVPKENKIIEGWVTRRGLGTSCADRIRRSN"
  },
  {
    "reference": "MSELAGRHVLLGVTGSIAAYKSPDIVRRLKDQGAEVRVVLTASAEKLVSPTVFQAVSGEPVRGDIWDEQAEAAMGHIELARWADQVLIAPATANLIAQLAAGSAENLLSTICLATEAPIALAPAMNQAMWRDPATQNNCEVLRNRNVRLIGPDEGSQACGDVGPGRMSEPVDIVNRLLRGGATGPFEGQTILVSAGPTREAIDPVRFVSNRSSGKMGFAMARAAADAGASVILVAGPVNLPTPPGVERIDVETTQQMFDAAMEQIDKADIYIGAAAISDYRPRKAATQKIKKSADTFTLEMVKSPDLLASISALDDGPFTVGFAAETEKLEEHAQSKLERKQLDMIIANLVGENICFDADDNEAVVLWSGGREALPRLSKSELARRLVDVIAGRYRHAKSASAA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must be able to perform FMN binding, phosphopantothenate--cysteine ligase activity under specific conditions.2. The Proton donor active site of the designed protein must be optimized for substrate binding and specificity.3. The CTP binding site should be located in a region of the protein that is accessible to the ligand.4. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A The designed protein sequence is ",
    "response#1": "MRILLGVTGGIAAYKAAELTRELQRRGCDVQIAMTASAERFVTPLTFASLSGHQVLTSLWQPAVSETASDTPAPFDIEHIAVATHIDALIIAPATANTLAKLAHGLADDLLSTIALATKAPVFVAPAMNVNMWHHPATQTNIATLRGRGVTVIEPGSGPLACGMVGEGRLADPIHIADTLAELVAAV",
    "response#2": "RVTLGVSAGISIYKACDLVRRLMDRNCEVQVVLTSHAARMVAPVTFQALSGRPVITDLFEGAGEPSIAHVELARWEELLVVAPATANVLGKFARGIADDFLTTHYLANRGAPVLLAPAMNGAMWRHPAVQENLRILEQRGHAVLQPGKGSLACGDVDEGRLPEPAAIAQEAVLQLLA",
    "response#3": "RVTLGVSAGISIYKACDLVRRLMDRNCEVQVVLTSHAARMVAPVTFQALSGRPVITDLFEGAGEPSIAHVELARWEELLVVAPATANVLGKFARGIADDFLTTHYLANRGAPVLLAPAMNGAMWRHPAVQENLRILEQRGHAVLQPGKGSLACGDVDEGRLPEPAAIAQEAVLALIG"
  },
  {
    "reference": "MKPESSFDESFTSVTIMAVSAIEDVIKDIQAGKMVILVDDEDRENEGDLYMAAEAVDAAAINFMARYGRGLICLTLSGDLVDKLGLPMMVPNNTSPYGTGFTISIEAKTGVSTGISAADRARTVQVAVDPESGPGDLVSPGHIFPLRARDGGVLVRAGQTEGSVDLSRLAGMIPAGVICEIMNEDGTMARMADLEEFSKEHDLKIATIADLVAYRLREDTLVKRQVEARVPTEHAGEFKAIVYSNSVDNLEHLALVKGDIENAEKVLVRVHSECLTGDVFGSSRCDCGLQLNAAMKMIDQAGCGIVVYMRQEGRGIGLVNKLKAYCLQDNEGVDTVEANLRLGFKSDLRDYGIGAQILRDLGVSKMSILTNNPKKIVGLEGYGIKVVERLPLEMPASEESKEYLMCKRDRMGHLIEVKKD",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that is capable of catalyzing reactions within the Cofactor biosynthesis pathway with high efficiency and specificity.2. The designed protein should have a binding site that is specific to the target D-ribulose 5-phosphate, GTP, Zn(2+), Mg(2+) ligand.3. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.4. The protein contains novel GTP cyclohydrolase II domains that confer a unique function or activity.5. The Proton acceptor; for GTP cyclohydrolase activity active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.6. A protein with GTP binding functions is required to enable riboflavin biosynthetic process. The designed protein sequence is ",
    "response#1": "RMAAAKLPTKHGTFQLFVYEVKYEQQQPVVLVMGDIATATAPLVRLHSSCFTGDLIDSLRCDCGDQLKLALEMISREGCGVLVYLPQEGRGIGLVEKVKAYGLQDQGMDTVEANHALGFKADMRDYGVGIQLLKDLGLTKVRLLTNNPKKTDAFIYGGFNLEVVDQVI",
    "response#2": "RMAAAKLPTKHGTFQLFVYEVKYEQQQPVVLVMGDIATATAPLVRLHSSCFTGDLIDSLRCDCGDQLKLALEMISREGCGVLVYLPQEGRGIGLVEKVKAYGLQDQGMDTVEANHALGFKADMRDYGVGIQLLKDLGLTKVRLLTNNPKKTDAFIYGGFNLEVVDQVK",
    "response#3": "RMAAAKLPTKHGTFQLFVYEVKYEQQQPVVLVMGDIATATAPLVRLHSSCFTGDLIDSLRCDCGDQLKLALEMISREGCGVLVYLPQEGRGIGLVEKVKAYGLQDQGMDTVEANHALGFKADMRDYGVGIQLLKDLGLTKVRLLTNNPKKTDAFIYGGFNLEVVDQVK"
  },
  {
    "reference": "MSRKKTRVTGVDALAEAFDAVNHADLLERLGVDIDRDLLVLALTHRSFANENGMLPNNERLEFLGDAVLGLAVANRLYELYPSSPESDISKMRASIVSRYGLADIAREINLGEFILLGKGELLTEGRSKDSILADTTEALLGAIFRQHGYEVARDVVLTLFHHKINHASAKGIHQDWKTTLQEELAQRKKPMVEYQTTSVGPDHDLLFTAVVYLGDVEMGRGEGPNKKLAEQEAARQAFLKLREKRA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S); Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.; Digests double-stranded RNA.2. The designed protein should contain one or more RNase III domains that are essential for its function.3. The designed protein should have ribonuclease III activity within cytoplasm to regulate mRNA processing, rRNA processing.4. The protein should have a high affinity for Mg(2+). The designed protein sequence is ",
    "response#1": "RRAFTHRSMNKLDEKGNPMNYERLEFMGDAMLGSVIAAHLFNMSPTGDEGYLTKMRSKIVSREHLNELGKDLNLIQFLESKVTLQNFGENIHGNLFEAFVGAIYLDRGFVYCEKFIHKKVIKC",
    "response#2": "MDGVDELLLRKFNLTLPPGLLRTAFVHKSFSFENGACQNNERLEFLGDAVLGLVVSHYLFETCPEYTEGQLSAARSYIVSGTSIAQIAKELNLGQFMLLGKGEKLAGGMDKTSLLADLLESLIGAVMVDQAVS",
    "response#3": "LKAFFKQYHVFIKERQYYLEALTHNSYANEHNLSYTYQRMEFLGDAILAKEISLYLFLSFPDKNEGEITNLRSKIVREGTLAELVRRMNWAPFLLLGKGEIKTKGYEKNRILADIYESMIAALYLDLNDAISKIIDYKFTNYAILEEALTHPSVNKRNSKNQIVSYERLEFLGDSVLNMVVSATLFKLFPEEKEGALAKRKTDLVCGNTIANVAKEIKLGSFIIMNNSERCNGGRCNLKNLENSLEALIGAIYIDGGLERFKREYLDPKTRLQEYLQGKRLALPAYNVTDIQGEAHCQTFTVECHVENLDRTFVGVAGSRRKAEQVAAEQILKVLDG"
  },
  {
    "reference": "MSTTIRTQDNLSAWERFGQWITSTNNRLYIGWFGVILIPTILTATICYIIAFIAAPPVDIDGIREPVSGSLLYGNNIISGAVVPSSNAIGLHFYPIWEAASLDEWLYNGGPYQLVVFHFLIGIFCWMGRQWELSYRLGMRPWICVAYSAPVSAATAVFLIYPIGQGSFSDGMPLGISGTFNFMIVFQAEHNILMHPFHMLGVAGVFGGSLFSAMHGSLVTSSLVRETSESESQNYGYKFGQEEETYNIVAAHGYFGRLIFQYASFNNSRSLHFFLGAWPVVGIWFTAMGISTMAFNLNGFNFNQSIIDSQGRAVATRSLRETATWADVINRANIGFEVMHERNAHNFPLDLASSDAMPMDLTAPAIDG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a fast turnover rate for 2 a plastoquinone + 2 H2O + 4 hnu = 2 a plastoquinol + O2.2. The designed protein should be able to compete with endogenous proteins or inhibitors for [CaMn4O5] cluster binding.3. The designed protein must be optimized for oxygen evolving activity. The designed protein sequence is ",
    "response#1": "CCFIEFASLIGSRFDFDRYGLVPRASPRQADLILTAGTVTMKMAPSLLRLYEQMPEPKYVIAMGACTITGGMFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAVIDAITKC",
    "response#2": "CCFIEFASLIGSRFDFDRYGLVPRSSPRQADLIITAGTVTMKMAPSLVRLYEQMPEPKYVIAMGACTITGGMFSTDSYSTVRGVDKLIPVDIYLPGCPPKPEAIIDAIIKC",
    "response#3": "CCFIEFASLIGSRFDFDRYGLVPRSSPRQADLIITAGTVTMKMAPSLVRLYEQMPEPKYVIAMGACTITGGMFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAIVDAIIKC"
  },
  {
    "reference": "MAAAALIRSTRCNTCRRYLLQSFTRVVGLTAQNSRLPNEPQRRQFFTTPYLNIKSSHRHLSDRDASAASNNEYFITNESANSSQAVPWYLQVEPVIEPTHPAVRQQEIPAVPANAPPITQEILQHLSLEIGLDDMVLLDLRGRDPPPALGGNVIMVIGTARSLKHLNVSADRFCRWLRSTYKLRPYADGLLGRNELKIKLRRKARRARLASSSGTISDTSDDGITTGWICVNVGSVDDPSVVQNMREGGFEGFGKVQGGTRIVVQMFTEDKRADVDLEQLWAPKDNVKAGDSEENHSYSTGGDYQVRSMPPITSENNSNHPISNVPRPLRRVDLSQRRSFSTGAGQHSARTDVDPESGYEVVDAKNESSRQEPPLSAIFSTLKEMSPEETIEQLGSGPEDKNSTEFLRDCYSSVGTSHNGNSALRLMLGAAGVSAQHPGYTKEYLWTLFLEQTASGYRISEPLGFEIASAFLVPRRLDAKEQGRTASEFVDFDIESAMRVMDNLSLNGETIIRKHRVYNMMYEALILGLRNERLDDVRLKALRIRTLMTEIGLDWQSEDTRETMQLRLLLGDVDGFYEIWHAIAFNEGSRTADDYELLFRVHAEAGNPVYARECLGVWISMMGREDPPVLPSASIISAIARCAHVAGHDADTFRLLSASDEWREVERLILSDLRTLEQLT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should emphasize Polar residues, Basic and acidic residues compositional bias.2. For general function, the protein need meet that Probable mitochondrial mRNA stabilization factor.3. The protein should be optimized for stability and solubility when expressed in mitochondrial inner membrane where it will be involved in mitochondrial gene expression. The designed protein sequence is ",
    "response#1": "PPALTAAGARYWSDYGRMIYGQDSVQELAHWHHDVAAPSAPDFEALGQRRFDRYENGYEVFTEECARDFFDISLHRQLEQCDTLQGFNLVTETDNGWGGFMAALQEQLREEVPKVCYFGWGLNVDESGPRHPPRAGFQRQSNKLRATLAMLEQSDLYFPIRSEAADSLWEAGGWACHVLDSC",
    "response#2": "EGKILCLVGPPGVGKTSIGKSIARALNRQYYRFSVGGLTDVAEIKGHRRTYVGALPGRIIQALKKCQTENPLILIDEVDKIGRGHQGDPSSALLELLDPEQNSSFLDHYMDVPVDLSKVLFVCTANVTDTIPRPLLDRMELIELSH",
    "response#3": "PPALTAAGARYWSDYGRMIYGQDSVQELAHWHHDVAAPSAPDFEALGQRRFDRYENGYEVFTEECARDFFDISLHRQLEQCDTLQGFNLVTETDNGWGGFMAALQEQLREEVPKVCYFGWGLNVDESGPRHPPRAGFQRQSNKLRATLAMLEQSDLYFPIRSEAADSLWEAGGWACHVLDSC"
  },
  {
    "reference": "MAKRVCVGDLRKLAGSVSTPSRCILPPHARCFSVLNRPPPNYEGHIPLTVIERLGLAVGSGLGSFLDPRRADLIASFGEATAQPHFIYKLRDRMLLNPTGRRILRDRPRLTSTSLDIPRLRQLPPNTLGYVYALWLDREGVSPDTRATVQYIDDEECAYVMQRYRECHDFYHALVGLPVFREGEVALKAFEFANTGLPMTGLAVFSAFTLKKAEWRRFWDIYGPWATRNGAQSDDVINIYWEEELETDIDQLRTRLGIEKPPDLREMRKAEREAQKKDKEAKETMTRAAV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in extrinsic component of mitochondrial inner membrane that is critical for ubiquinone biosynthetic process.2. The designed protein should interact with enzymes and substrates within the Cofactor biosynthesis pathway to enhance or regulate its activity.3. For general function, the protein need meet that Required for steady-state levels of other COQ polypeptides.; Component of the coenzyme Q biosynthetic pathway The designed protein sequence is ",
    "response#1": "RVQNSANHMPQRQLEKVMAKELGANWKTKFSKFDKIPMAAASIGQVHAAELPSGQRVVVKIQYPGVKESIDSDLNSLLMLLTASSLLPKGLFLDKTIANARTELKWECDYNREARALQKFEALLKDDPAFEVPHVFPEYTTDNIITMTRMEGTEIMKLPKASQETKNFISENIMRLCLEEIATFKYMQTDPNWANFLYNGRTKKIELLDFGASRPFAEDFILKYRKLLTYATLRDRKGAYEC",
    "response#2": "EILSRVQNSANHMPQRQLEKVMAKELGANWKTKFSKFDKIPMAAASIGQVHAAELPSGQRVVVKIQYPGVKESIDSDLNSLLMLLTASSLLPKGLFLDKTIANARTELKWECDYNREARALQKFEALLKDDPAFEVPHVFPEYTTDNIITMTRMEGTEIMKLPKASQETKNFISENIMRLCLEEIATFKYMQTDPNWANFLYNGRTKKIELLDFGASRPFAEDFILKYRKLLTYATLRDRKGAYEMSVQLF",
    "response#3": "EILSRVQNSANHMPQRQLEKVMAKELGANWKTKFSKFDKIPMAAASIGQVHAAELPSGQRVVVKIQYPGVKESIDSDLNSLLMLLTASSLLPKGLFLDKTIANARTELKWECDYNREARALQKFEALLKDDPAFEVPHVFPEYTTDNIITMTRMEGTEIMKLPKASQETKNFISENIMRLCLEEIATFKYMQTDPNWANFLYNGRTKKIELLDFGASRPFAEDFILKYRKLLTYATLRDRKGAYEMSVQLY"
  },
  {
    "reference": "MENRWQVLIVWQVYRMKIIAWNTLVKHHMYVSRRSIGWFYRHHYESRHPKISSEVHIPLGDARLIITTYWGLQTGQRDWHLGHGVSLEWRVGIYTTQIDPDLTDQLIHVHYFDCFAKSAIRKAILRQIVRPRCDYQAGHHKVGSLQYLALSALIKPKKTKPPLPSVSKLLEDKWNKPQKTRCRRGNHITNGH",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a specific function within the plasma membrane, host cell plasma membrane.2. The protein must exhibit the following characteristics: Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G; Finally, Vif also contributes to the G2 cell cycle arrest observed in HIV infected cells.; May interact directly with APOBEC3G mRNA in order to inhibit its translation.3. The protein's functional design necessitates the inclusion of a BC-box-like motif motif. The designed protein sequence is ",
    "response#1": "GALGFLL",
    "response#2": "GQAQK",
    "response#3": "GCCNIL"
  },
  {
    "reference": "MVASCKDQKKAVAICLQRSPCVMIERHNPQECLDNPELNKDLPELCIAQMKAFLDCKRGIVDMTKRFTGNAPLSTGKYDQQYENLCKGKFDPREEMEKLKLLNSQQKD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design must feature the Cx10C motif motif.2. The designed protein must have at least one CHCH domain. The designed protein sequence is ",
    "response#1": "ELHMAIRAIGSIIRDYTPNRLFAAFGIGAKIPPTFHESNEFFLNFSMDPICRGLDGVMEAYRKTQTMVTPLKESKLSPVINYVTRMSHRSGFRGLHYHVLALFTRGEVTDLKEIQQALNSASDAPLSILIIGMGDFDFSSLQKLCSKRKDGRRDVVQFIHLKSLLNGAEAPWLNKSRIAETALRHVPQHMVQYMHNANIC",
    "response#2": "ELHMAIRAIGSIIRDYTPNRLFAAFGIGAKIPPTFHESNEFFLNFSMDPICRGLDGVMEAYRKTQTMVTPLKESKLSPVINYVTRMSHRSGFRGLHYHVLALFTRGEVTDLKEIQQALNSASDAPLSILIIGMGDFDFSSLQKLCSKRKDGRRDVVQFIHLKSLLNGAEAPWLNKSRIAETALRHVPQHMVQYMHNANIC",
    "response#3": "ELHMAIRAIGSIIRDYTPNRLFAAFGIGAKIPPTFHESNEFFLNFSMDPICRGLDGVMEAYRKTQTMVTPLKESKLSPVINYVTRMSHRSGFRGLHYHVLALFTRGEVTDLKEIQQALNSASDAPLSILIIGMGDFDFSSLQKLCSKRKDGRRDVVQFIHLKSLLNGAEAPWLNKSRIAETALRHVPQHMVQYMHNANIL"
  },
  {
    "reference": "MEGFGEFESRPSKDKIKILKGTTTVGLVVKDAVILAADRRASAGFFVANKMVRKIVYIDDRIGMTTAGSVADLQFIYNYMKNIYHYNLISGNRPVTVKSLATYLTNILSQNKYFPYLVQILMGGYDTQPRLFNLDYLGDMTEETYVATGSGSPVAMGVLEDGYSPDLTGDQAMDLAARAVMSAIKRDSFTGTGVIVTKITKEGHVEREIYPNRKEAM",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should aim to mimic or enhance the function of natural proteins involved in proteasomal protein catabolic process, potentially with improved properties such as stability, specificity or selectivity.2. The protein should be designed to catalyze the following reaction Cleavage of peptide bonds with very broad specificity.. The designed protein sequence is ",
    "response#1": "MF",
    "response#2": "MGTF",
    "response#3": "SKTR"
  },
  {
    "reference": "MSFLKELFSRYTLHEGQSKSAELAYFFLLSLFPFLIFMLTLTAYLPLSTDDVLGVIEQYAPASAMSLVESITHQTLNNRNGGLLSFGIIAALWSASNGMNAIVRSLNHAYDVEENRSFIIVRLTSIFLTIAMVFTILVALLLPVFGREIGRLASDFVGASDLFLSVWAAIRWGVSPLVLLIVFSALYVIAPNKKLSLRFVMPGAVFATIGWIIVSTLFSFYVSTFANYSATYGSIGGIIVLMIWFYLSGILIILGGEINALLHKRKKLPDENPYH",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The protein can be targeted to plasma membrane and perform exonuclease activity. The designed protein sequence is ",
    "response#1": "MF",
    "response#2": "LHHHHHHPD",
    "response#3": "MNLLK"
  },
  {
    "reference": "MLLLINVILTLWVSCANGQVKPCDFPDIKHGGLFHENMRRPYFPVAVGKYYSYYCDEHFETPSGSYWDYIHCTQNGWSPAVPCLRKCYFPYLENGYNQNYGRKFVQGNSTEVACHPGYGLPKAQTTVTCTEKGWSPTPRCIRVRTCSKSDIEIENGFISESSSIYILNKEIQYKCKPGYATADGNSSGSITCLQNGWSAQPICINSSEKCGPPPPISNGDTTSFLLKVYVPQSRVEYQCQPYYELQGSNYVTCSNGEWSEPPRCIHPCIITEENMNKNNIKLKGRSDRKYYAKTGDTIEFMCKLGYNANTSILSFQAVCREGIVEYPRCE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be involved in complement activation by localizing to extracellular space, blood microparticle, extracellular exosome and exhibiting complement component C3b binding.2. For general function, the protein need meet that Might be involved in complement regulation.3. The designed protein have Sushi domains with specific sequence motifs that are crucial for its activity.4. The protein must contain a signal peptide for proper functionality. The designed protein sequence is ",
    "response#1": "MSVNASDQERTPWQVTFKPKSKETCQGSLISDQWVLTAAHCFHDAQMEDRHLWRVIVGDPTSHHGKEFHVEEVLVAPGFNVHAKQSQGISEFYADDIALLKLSQRVKMSSHARPICLPCTVGANMALRRSPGSTCKDHETELLSQQKVPAHFVALNGNRMNINLRTGPERTSCIEAVSQNKGSFPGLTNVSEVVTDQFLCSGMEGDDSPCKGESGGAVFLERRYRFFQVGLVSWGLFDPCQ",
    "response#2": "SDQERTPWQVTFKPKSKETCQGSLISDQWVLTAAHCFHDAQMEDRHLWRVIVGDPTSHHGKEFHVEEVLVAPGFNVHAKQSQGISEFYADDIALLKLSQRVKMSSHARPICLPCTVGANMALRRSPGSTCKDHETELLSQQKVPAHFVALNGNRMNINLRTGPERTSCIEAVSQNKGSFPGLTNVSEVVTDQFLCSGMEGDDSPCKGESGGAVFLERRYRFFQVGLVSWY",
    "response#3": "GVGNMSANASAQERTPWHVTIKPKSQETCRGALISDQWVLTAAHCFRNAEDRTLWRVSVGDPNFQGSKEFQIEEAVISPGFNVFSKKSQGIPEFYGDDIALLKLTQKVKMSTHARPICLPCTVGANLALRKLPGSTCRDHEKELLNQVSIPAHFVALNGDKLNINLKTGSEWTNCVKVVLKDKTTFPNLTDVREVVTDQFLCSGTQGDDSPCKGESGGAVFLERRLRFFQVGLVSWGLYNPCGGSSKNSRKPAPHGKVPRDFHINLFRLQPWLRQHLR"
  },
  {
    "reference": "MNGTMNFQEIILNLQRFWAKKGCVLVQPYDMEVGAGTFHPETLLRALGPEPWQVAYVQPSRRPTDGRYGENPNRLQHYYQFQVMLKPSPGNVQQLYLKSLATLGIFASDHDIRFVEDDWESPTLGASGLGWEVWLDGMEITQFTYFQAAGSIDLSPVSVEITYGLERIAMYMQGINNVYDLVWRDHITYGHLHHRQEVEQSAYNFEHADVEMLFDCFGKYEHEAGRMIAESLVLPAYEYCLKCSHTFNLLDARGAISVTERTGYIKRIRDIARACATTYLQQREEMGFPLLNAGHRGTTE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The desired protein should be able to perform ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-   tRNA(Gly) The designed protein sequence is ",
    "response#1": "EDAQSEIFLRPETAQGIFVNFKNAIRTTRRKMPFGMAQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTDLEWFEYWRDYSWNFLLSLGLAKDNLRIREHAKEELAFYSKATVDIEYLFPFGWGELWGIADRTDYDLKQHMNHSGKDLTYLDPETNEKYLPYCIEPSLGADRVALY",
    "response#2": "KAAILPLTKKLKDKSLEVYSMLSKYFNVDYDEAGTIGKRYRREDEIGTPYCITIDYDTLEDNTVTIRDRDSMDQIRINIDELVKYISQ",
    "response#3": "KAAILPLTKKLKDKSLEVYSMLSKYFNVDYDEAGTIGKRYRREDEIGTPYCITIDYDTLEDNTVTIRDRDSMDQIRINIDELVKYISG"
  },
  {
    "reference": "MRIVLGVAGGIAAYKAVLLLRLLSEAGHDVRVVPTESALEFVGRATWEALSGEPVTTRVFEDVDQVPHVRLGKTADLVVVAPATADLLSRAATGRADDLLTATLLTARCPVLLAPAMHTEMWEHPATRANVETLRARGVHVLDPDSGRLTGADTGPGRLPEPEAIAAAALALVRTAPQDLAGRHVVVSAGGTREPLDPVRYLGNRSSGRQGYALARAAAARGAQVTLVAANVTLPAPDGVAVVPVETSAELRDAVRAAAKDADVVVMAAAVADFRPATSAETKIKKDADGATRALELVQTTDILAELSAPRLRAGQLVVGFAAETGDAEGTVLDHGRAKARRKGADLLVVNAVGGGRGFGTADNDVTILDGNGDVVAVAAGSKDDVAHAVWDAVLLRG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. We require a protein that can specifically bind to the metabolites produced in the CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis, coenzyme A biosynthesis pathway.2. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domain.3. The protein have enhanced H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.5. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A.6. A protein with phosphopantothenoylcysteine decarboxylase activity, phosphopantothenate--cysteine ligase activity, metal ion binding, FMN binding is needed to enhance coenzyme A biosynthetic process, pantothenate catabolic process in a specific context. The designed protein sequence is ",
    "response#1": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALALAARLLGGHGGLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHVLGADGTEEPVPYGPKEALADTVWDLVAARLPT",
    "response#2": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALAAALDLLAPRLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHLLRAGADEPLEGVRVVVSAGGTREPLDPVRYLGNRSSGKQGYALARVAAQRGAAVTLVAAHTLALPEPAGVDLVSVSTAEQLGKAVRTAAADADVVVMAAAVADFRPESLAEHKIKKADDGSAPVVTLARNPDILAGLVADRRPGQLIVGFAAETGDERGGVLDHARRKLARKGCDLLVVNAVGEGRAFEVEENTGWLLSADGTTTELAHGSKRDVADRVWDLIAGRLS",
    "response#3": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALAAAHDLLGAPRDLLAGQRVVITAGPTREPLDPVRYISNHSSGKMGYALARAAVEAGASVQLVSGPVALAVPDRVHCHPITTAREMLDVCMDLVENCDIFIGCAAVADYRPMVESEQKIKKSNEEMSLQLTRNPDILASVAGHSAKPFSVGFAAETEKLREHALGKLDRKGLNMIVANDVSDSSIGFNSENNAALLFWKDGSADAWPRMGKDEVARRLAARIAEALG"
  },
  {
    "reference": "MEVQELFLLALAISLDAFGVILCIGINKGITLKSSIIFVFSFGFFQFFLSFLGGYIGTIFNKYIVPIPTIVGGLIIIIVGILMITEGFKEKEESIFLNKIMYLILGVSVSIDALVIGFTTLSYINNLFYLFMSSLFMGLIATIICSLGIILSKYIKKISIISSYADYIGGIILILFGLKMLFF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for plasma membrane-specific folding and stability, while maintaining manganese ion transmembrane transporter activity.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. For general function, the protein need meet that Probably functions as a manganese efflux pump. The designed protein sequence is ",
    "response#1": "LTKQ",
    "response#2": "GLLPTF",
    "response#3": "GFSS"
  },
  {
    "reference": "MSLCQGSAMQKLIAKTEGPMAGVGRVGGFNRPSGGLGQSSAEQQLQARAGERASQNRFMAVLEPQRELGGRMARGDGLQADWVRQFSSMQVEDPLAFSAEYQRAYAGYEQRQAARPAARVLGYGGSMFMPTMPQQQLQQQVAPQQTAQAALQEAELERYLEREFDVLEGELAPPEVPEALSAPLLDHEQLGFQESAKAIYATLSAPIHKDKFGASKFMGLMRQVSTGDVTLSKSESGYTGLHMTAGGEAVGAEYRAVTDEVVQVPEVAAALPLAGEERSLASQMEDLLNKVDIAGLSSNEAAMRILS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Interaction with PEX7 only takes place when PEX7 is associated with cargo proteins containing a PTS2 peroxisomal targeting signal . The designed protein sequence is ",
    "response#1": "AGSLAQGSEATFQLIESIIGAVGGFAQVLEATYMATHSSFFTMISMADQLSHLKTALGSMLGIYTVINWLKRIMGKLMGVKNKLTPDEFRKFQEKQMKKLSNSNNTGGPNKNTNKLSLKPLLLFLAAVVGFPYLLKKLI",
    "response#2": "AGSLAQGSEATFQLIESIIGAVGGFAQVLEATYMATHSSFFTMISMADQLSHLKTALGSMLGIYTVINWLKRIMGKLMGVKNKLTPDEFRKFQEKQMKKLSNSNNTGGPNKNTNKLSLKPLLLFLAAVVGFPYLLKKLF",
    "response#3": "AGSLAQGSEATFQLIESIIGAVGGFAQVLEATYMATHSSFFTMISMADQLSHLKTALGSMLGIYTVINWLKRIMGKLMGVKNKLTPDEFRKFQEKQMKKLSNSNNTGGPNKNTNKLSLKPLLLFLAAVVGFPYLLKKLF"
  },
  {
    "reference": "MAPEHRLFSHSLPQSLWRAEVVKRQEAVLAEQQGISLYSLMEQAGLAAFKLMQCQWPNAKRILVLAGGGNNGGDALVVARLAKQAGKEVKVLALGDLDRMPSDAQQALAAWQAEGGQLDLSKQFDWPCDVIIDGVLGIGLNSDVRAPLRSLFAQVNQSGVPVLALDLPSGLSADTGKLLGSAIKASATICFIAAKQGLFTGQAPEYVGKLLFAGLGLSDLFEQQNTTEVSRVDYAQLRSMLAPRAKIAHKGSCGRVTLVGGNTGMAGAIRMAAEACLRTGAGLVNVFTQAQNQSVVSSGRPELMVAAVTKHSLQLLDRSLIQASCKVIGPGLGQDEWAQALFSAVLSEDKACLVDADALNLLAKAPMQHEQWVLTPHPGEAARLLNCSVAEVESDRIAAAQNIQRCFGGVCVLKGAGSVVAGAQGQVKICTVGNPGMASGGMGDVLSGIIGGLIAQFAAKHSLFDIACLGVCIHGMAADEAAEQGERGMLASDLMPFIRQLVNPKL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.2. A protein with ATP binding, NADPHX epimerase activity, NADHX epimerase activity is needed to enhance nicotinamide nucleotide metabolic process in a specific context.3. The desired protein should be able to perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate4. The AMP, (6S)-NADPHX, K(+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MQRLEMAAVTAGITLAQLMENAGAALAQEVESRCRPIQGKRAVLLCGKGNNGGDGFVCAGLLAQRGMTCTVVLTQGEPKTDLAGDAFSRMPDQVQVISGAEEREQAEAAMTAADVIVDCVFGFSFRGELSGVPAHFLGYAGNLSCLKISADLPSGVECDSGRVSAGAFRADVTVTFTGKKPANVSYPAKEFCGETVVRQVGVPAALVGPELWLAALPFPAVDAHKYARGHACVFSGGPSATGAARLAALAAARAGAGAVTVLSPPAAMQVNAAHLTAIMLRRIASEGELIDFARERKPSSYAIGPGFGVGENARSFILSLLGRKDADCNGMVVDADAITSFRDDPALLFAAAASSGAPSLVLTPHAGEFRRLFPDLAEGEAGSKLEAARRAARRANAVVVYKGPDSVIAAPDGRARINSNGAPFLATAGSGDVLAGIIAGLMAQGMSAFDAASAAVWIHAEAGSRFGAGLIAGDLPRLLPAILSELFI",
    "response#2": "MAMADAAAIAGGVPGADLMEAAGRAVAEAVLERHLPRPVVVLCGPGNNGGDGFVAARHLHAAGWPVRVALLGDRSALHGDAASASQAWKGPVEALSLGLLDGHPLVIDALFGAGLGRPIDGIAGELIDRMNAEALHVVSVDIPSGLHGDSGEVMGCAPMAECTVTFFHAKPGHYSVEGLRRCGSLRVVDIGIARSVLGPELWLAALPFPAVDAHKYARGHACVFSGGPSATGAARLAALAAARAGAGAVTVLSPPAAMQVNAAHLTAIMLRRIASEGELIDFARERKPSSYAIGPGFGVGENARSFILSLLGRKDADCNGMVVDADAITSFRDDPALLFAAAASSGAPSLVLTPHAGEFRRLFPDLAEGEAGSKLEAARRAARRANAVVVYKGPDSVIAAPDGRARINSNGAPFLATAGSGDVLAGIIAGLMAQGMSAFDAASAAVWIHAEAGSRFGAGLIAGDLPRLLPAILSELFSRERRADTHKGSYGHTLIAAGTKRMSGAGLLCAKAALRAGCGLATWAVPASLATHLLGRVPEAMLAGLADAGHGDWTHVNAEAILALAEDCDALALGPGMGRYEGDSQWLRGLWEGAGCPLVLDADALNMLAEAPDFADWQPRRAATVLTPHPGEMARLAGVSTREVQRDRIGLARRYAEQHGVTLVLKGARTVVATPDGAVYVNVNGNAGMATAGAGDVLAGIIASLLSQGFDAAQAACLGVYMHGAAGDRAALKRTSPASLLAGDLLELP",
    "response#3": "MLRPLYTADEMRAAEQGHDVEAMMERAGSAVAETVLRRYPDARRIAAVCGKGANGGDGRIALRVLAEAGRETGEELDGADVVIDALFGTGFHGAPRDDAAGRIEQINGAGVPIVAVDLPSGVDASTGEVVGAVVDADVTVTMHGPKVGLEVGPGRFHAGEVEVAEIGLGPELWLAALPFPAVDAHKYARGHACVFSGGPSATGAARLAALAAARAGAGAVTVLSPPAAMQVNAAHLTAIMLRRIASEGELIDFARERKPSSYAIGPGFGVGENARSFILSLLGRKDADCNGMVVDADAITSFRDDPALLFAAAASSGAPSLVLTPHAGEFRRLFPDLAEGEAGSKLEAARRAARRANAVVVYKGPDSVIAAPDGRARINSNGAPFLATAGSGDVLAGIIAGLMAQGMSAFDAASAAVWIHAEAGSRFGAGLIAGDLPRLLPAILSESHATNAFPLPRRARDAHKGNFGHVLVIGGDVGFAGAPLLAGEAAARVGCGLVTLATRPQHAGMQVAARPELMAWGVDRPAQLDPLLDRASVVAIGPGLGQSPWAAGLLARVLETNLPLVVDADALTLLARDPVQNERWVLTPHPGEAARLLDCSTAEIQGDRFTAVRELQSRYGGAIVLKGCGSLVATRKSGVTVCTDGNPGLASGGTGDVLTGVIAGLMAQGLEPESAALTGACLHGAAADRAVLEGERGMLAGDLMPWLRRLVNPDP"
  },
  {
    "reference": "MNEKKNIRTLSLEELTAYFATINEKKFRAKQVWEWLWQKHATNFEDMTNLGKELRQNLSDNFSLPAIHTDASQYSSDGTVKTRFKTYDGHLVEGVLIPVDNRQTACVSSQVGCSLSCKFCATGYMDRKRNLHFDEIYDEVVLLNEMAEKTYGKKLSNIVFMGMGEPLLNYKNVLKAIERITANDGLGMSSRRITVSTAGVAKMIKQLGDDGVKFNLALSLHAANDKKRNEIMPINETNNIKALIEALNYFYDKTKNDITFEYILFKGENDSIQDADDLIKIYKQVPVHLVNIIEYNPIDNATFVKPDKDATQKFMDYLAAHRVNVRLRISRGKDIDAACGQLANKNLEPSAVN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity.2. The protein must have high specificity towards rRNA (adenine-C2-)-methyltransferase activity. The designed protein sequence is ",
    "response#1": "SSQAGCGMACPFCATGQGGLQRNMSTGEIVAQVMKAEQVLSSGELPGGAGQVNNIVFMGMGEPLANYKAVMGAVRAISDPTPDGLGMSKRGITVSTVGLVPRIEQLTAEGLPITLAISLHAPDDELRDELVPINTRYKVDEVLDAAWDYAKTTKRRVSIEYIMIKDINDQPERF",
    "response#2": "SSQAGCGMACPFCATGQGGLQRNMSTGEIVAQVMKAEQVLSSGELPGGAGQVNNIVFMGMGEPLANYKAVMGAVRAISDPTPDGLGMSKRGITVSTVGLVPRIEQLTAEGLPITLAISLHAPDDELRDELVPINTRYKVDEVLDAAWDYAKTTKRRVSIEYIMIKDINDQPERL",
    "response#3": "SSQAGCGMACPFCATGQGGLQRNMSTGEIVAQVMKAEQVLSSGELPGGAGQVNNIVFMGMGEPLANYKAVMGAVRAISDPTPDGLGMSKRGITVSTVGLVPRIEQLTAEGLPITLAISLHAPDDELRDELVPINTRYKVDEVLDAAWDYAKTTKRRVSIEYIMIKDINDQPERLKPAKHWIDLSTEERVQAVKDLGLPAFRAQQISKHFFERFETDPTTWSDLPKAAREQVAERFFPELVTPMSEQRCDGDTTVKTLWRLHDGSLVESVLMRYGVKRGLPEDQQSHVRSTLCISSQAGCGMACPFCATGQGGLQRNMSTGEIVAQVMKAEQVLSSGELPGGAGQVNNIVFMGMGEPLANYKAVMGAVRAISDPTPDGLGMSKRGITVSTVGLVPRIEQLTAEGLPITLAISLHAPDDELRDELVPINTRYKVDEVLDAAWDYAKTTKRRVSIEYIMIKDINDQPERAALLAKRLKARGDWGWVHVNLIPLNPTPGSKWTASRKSDEQAFVKTLENWGIPVTVRDTRGQEIDGACGQLAAAS"
  },
  {
    "reference": "MQALRVSQALIRSFSSTARNRFQNRVREKQKLFQEDNDIPLYLKGGIVDNILYRVTMTLCLGGTVYSLYSLGWASFPRN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation; Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water; Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B))2. The designed protein must be able to regulate mitochondrial respirasome assembly. The designed protein sequence is ",
    "response#1": "SSPIMEELVEFHDHALMVALAICSLVLYLLTLMLMEKLSSNTVDAQEVELIWTILPAIVLVLLALPSLQILYMMDEIDEPDLTLKAIGHQWYWTYEYTDFKDLSFDSYMTPTTDLPLGHFRLLEVDHRIVIPMESPIRVIITADDVLHSWAVPALGVKTDAIPGRLNQTSFITTRPGVFYGQCSEICGANHSYMPIVVESTPLKHFEAWL",
    "response#2": "MWKERWFLSTNAKDIGTLYLMFRYFSGLVGTAFSVLIRLELSAPGVQYIADNQLYNSIITAHAILMIFFMVMPALIGGFGNFLLPLLVGGPDMAFPRLNNISFWLLIPSLLLFVFASIIENGAGTGWTLYPPLASIQSHSGPSVDLAIFGLHLSGISSLLGAMNFITTIINMRSPGIRLHKLALFGWAVLITAVLLLLSLPVLAGAITMLLTDRNFNTSFFELAGGGDPIFIQHLFWFFGHPEVYILIVPGFGIISTVISANSSKNVFGYLGMVYAMMSIGILGFVFWSHHMYTVGLDVDTRAYFIAATLIIAVPTGIKIFSWLATCYGGSLNLTPAMLFALGFVVMFTIGGLSGVVLANASLDIAFHDTYYVVAHFHYVLSMGAVFALFSGWYFWIPKLLGLSYDLFAGKVHFWILFVGVNLTLFPQHFLGLQGMPRRIGDYPDAFAGWNLISSFGSIVSVVATWYFLNILYLQLTQGSPVSRYPWLTPQYFSDLFQHLFTRNNSSLEWCLNSPPKPHAFDCLPVQV",
    "response#3": "MWKERWFLSTNAKDIGTLYLMFRYFSGLVGTAFSVLIRLELSAPGVQYIADNQLYNSIITAHAILMIFFMVMPALIGGFGNFLLPLLVGGPDMAFPRLNNISFWLLIPSLLLFVFASIIENGAGTGWTLYPPLASIQSHSGPSVDLAIFGLHLSGISSLLGAMNFITTIINMRSPGIRLHKLALFGWAVLITAVLLLLSLPVLAGAITMLLTDRNFNTSFFELAGGGDPIFIQHLFWFFGHPEVYILIVPGFGIISTVISANSSKNVFGYLGMVYAMMSIGILGFVFWSHHMYTVGLDVDTRAYFIAATLIIAVPTGIKIFSWLATCYGGSLNLTPAMLFALGFVVMFTIGGLSGVVLANASLDIAFHDTYYVVAHFHYVLSMGAVFALFSGWYFWIPKLLGLSYDLFAGKVHFWILFVGVNLTLFPQHFLGLQGMPRRIGDYPDAFAGWNLISSFGSIVSVVATWYFLNILYLQLTQGSPVSRYPWLTPQYFSDLFQHLFTRNNSSLEWCLNSPPKPHAFDCLPVQV"
  },
  {
    "reference": "MGEPGGAEAAVSARLLELAADDNAAGLGELLAAWPSLADEPAPWYTPARGAEPLTPLMVAAVYGSVGCLDALLSPPYLVDPNRASASSLSTPLHLAAAGGSASAPAAVSRLLAAGADPALLDHLQRRASDLVALPPNSLPLKNHLLSLLGARKEWPPDPSLPDIKNGAYASDDFRMYSFKVRACSRAYSHDWTECPFVHPGENARRRDPRKYHYSCVPCPEFKKGAGCRRGDMCEYAHGVFESWLHPAQYRTRLCKDGVGCARRVCFFAHTPDELRPLYVSTGSAVPSPRGALEMAAAAAAMGMGLSSPGSSSFTPPLSPSAGGGGGGGGGSGGGGAWPQQPSVPALCLPGSAGNLHLSRLRTSLSARDMAVDELLAAAAAAADYDGLVASPASIRSARGKALVPSNLDELFSAELAAAAASRSPRYADQGGAAFSPTRKATVLNQFQLQQQHSLLSPRAAAVTPEPVSPMSSRLLAALAQREKMQQQTLRSMSSRDLGNAASLLVGSPVSSSMSKWGFPSGNPDWGADDEELGRLKRCSSFELRSGAANGNHEPDLSWVNTLVKEPTPEKMMTTTSAMDSIGILGQNTSRDHIVGGEDDTAGVISSWLEQLQLDEMVV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must possess DNA binding, metal ion binding to support regulation of nitrogen compound metabolic process, regulation of primary metabolic process, regulation of macromolecule metabolic process under certain conditions. The designed protein sequence is ",
    "response#1": "GPALWRHALPKPGLSSHKYERGHTLVASGGATRTGAARLAARAALRIGSGLVTVASPPEALGVNAAQLTAVMLRGCDGPEGLHAILQDPRFNALVLGPALGVHAATRAMVSVAVHARRSLVLDADALTSFEGLASELHGAFRHAPTVLTPHGGEFNRLFKGHPDILDPESKIERTRRAAAYTGAVVLLKGADTVIAAPDGRAAINENGTPYLATAGSGDTLCGLIAGLMAQGLPAFEAACAGAWIHAEAGAQFGPGLISEDLAEQVPAVLRER",
    "response#2": "SHKYERGHTLVASGGATRTGAARLAARAALRIGSGLVTVASPPEALGVNAAQLTAVMLRGCDGPEGLHAILQDPRFNALVLGPALGVHAATRAMVSVAVHARRSLVLDADALTSFEGLASELHGAFRHAPTVLTPHGGEFNRLFKGHPDILDPESKIERTRRAAAYTGAVVLLKGADTVIAAPDGRAAINENGTPYLATAGSGDTLCGLIAGLMAQGLPAFEAACAGAWIHAEAGAQFGPGLISEDLAEQVPAVLS",
    "response#3": "SHKYERGHTLVASGGATRTGAARLAARAALRIGSGLVTVASPPEALGVNAAQLTAVMLRGCDGPEGLHAILQDPRFNALVLGPALGVHAATRAMVSVAVHARRSLVLDADALTSFEGLASELHGAFRHAPTVLTPHGGEFNRLFKGHPDILDPESKIERTRRAAAYTGAVVLLKGADTVIAAPDGRAAINENGTPYLATAGSGDTLCGLIAGLMAQGLPAFEAACAGAWIHAEAGAQFGPGLISEDLAEQVPAVLE"
  },
  {
    "reference": "MDALQYYPWLYIGLASLFGLLVGSFLNVVIYRLPKIMELEWRQECAESFPEYNITPPTETLTLSTPRSSCPSCHTPIRVRDNIPVFSWLALRGKCHHCQTKISARYPFVEALSAFLCGLVAWKFGYAPITVALIGFTLVLIAATFIDLDTMLLPDQLTLPLTWTGIALALLEISPVSLQDSVFGAMAGYLCLWSVYHLFRLLTGKEGMGYGDFKLLAALGAWLGWQYLPMIILLSSVVGLIFGLIQLRLQKQGIEMAFPFGPYLAIAGWVALMWGDSLMSWYLNYLIGA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with aspartic-type endopeptidase activity functions is required to enable proteolysis, methylation.2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.3. The designed protein should have a binding site that is specific to the target Zn(2+) ligand.4. The protein should be designed to have high stability and activity towards Typically cleaves a -Gly-|-Phe- bond to release an N-terminal,   basic peptide of 5-8 residues from type IV prepilin, and then N-   methylates the new N-terminal amino group, the methyl donor being S-   adenosyl-L-methionine..5. The protein must exhibit the following characteristics: Plays an essential role in type IV pili and type II pseudopili formation by proteolytically removing the leader sequence from substrate proteins and subsequently monomethylating the alpha- amino group of the newly exposed N-terminal phenylalanine.. The designed protein sequence is ",
    "response#1": "TMRRAGLVVAEGLEAMRAVAVPGATTADIDRAGRDVLEQHGARSNFLNYGGDWGLPPYPGVACVSPNETIVHGIPGERELKDGDIVSIDYGAIVDEWHGDAARTVLVGDVSEEARTLSEVTRESMWAGIAKVAPGARIGDISAAVQASLESHDGDYGIIREYTGHGIGTEMHMDPDVPNWGRAGRGPKIVEGMVLCIEPMATLGTEVTATLDDEWTVVTVDGSWASHWENTVAVL",
    "response#2": "TMRRAGLVVAEGLEAMRAVAVPGATTADIDRAGRDVLEQHGARSNFLNYGGDWGLPPYPGVACVSPNETIVHGIPGERELKDGDIVSIDYGAIVDEWHGDAARTVLVGDVSEEARTLSEVTRESMWAGIAKVAPGARIGDISAAVQASLESHDGDYGIIREYTGHGIGTEMHMDPDVPNWGRAGRGPKIVEGMVLCIEPMATLGTEVTATLDDEWTVVTVDGSWASHWENTVAVLKP",
    "response#3": "YFGEISIGTPPQNFLVLFDTGSSNLWVPSVYCQTQACTGHTRFNPSQSSTYSTNGQTFSLQYGSGSLTGFFGYDTLTVQSIQVPNQEFGLSENEPGTNFVYAQFDGIMGMAYPSLAMGGATTALQGMLQEGALTSPVFSFYLSNQQGSQNGGAVIFGGVDNSLYQGQIYWAPVTQELYWQIGIEEFLIGGQASGWCSQGCQAIVDTGTSLLTVPQQYMSALLQATGAQEDQYGQFFVNCNYIQNLPTFTFIINGVQFPLPPSSYILNNNGYCTVGVEPTYLPSQNGQPLWILGDVFLRSYYSVYDMGNNRVGFES"
  },
  {
    "reference": "MADYLLNALQWIDGLGTWAAIAFMLLYTVATVVFLPGSILTLGAGVVFGVILGSIYVFIGATLGATAAFLVGRYLARGWVAKKIAGNQKFKAIDEAVGKEGLKIVILTRLSPVFPFNLLNYAYGITNVSLKDYVIGSLGMIPGTIMYVYIGSLAGSLATLGTATNQANPTLQWTIRIVGFIATVAVTIYVTKIARKALNEAILTSEVDE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have a specific function within the plasma membrane.2. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "GRTANGKVRLYVIGQGALSAAVAANSKHDIAEAERWNLPIILIVLLAVFGSLAAAAVPLALGVCTVVVTMGLVDLVSMHTIMSVFVTSTVSMFGIALAVDYSLFILMRFREELRSGRQPQEAVDAAMATSGLAVVLSGMTVIASLTGIYLINTAALKSMATGAILAVAIAMLASITLTPAALATFGRAAVKRSVLMHWSQRSECTQSLFWTRWVGWVMHRR",
    "response#2": "GRTANGKVRLYVIGQGALSAAVAANSKHDIAEAERWNLPIILIVLLAVFGSLAAAAVPLALGVCTVVVTMGLVDLVSMHTIMSVFVTSTVSMFGIALAVDYSLFILMRFREELRSGRQPQEAVDAAMATSGLAVVLSGMTVIASLTGIYLINTAALKSMATGAILAVAIAMLASITLTPAALATFGRAAVKRSVLMHWSQRSECTQSLFWTRWVGWVMHRR",
    "response#3": "GRTANGKVRLYVIGQGALSAAVAANSKHDIAEAERWNLPIILIVLLAVFGSLAAAAVPLALGVCTVVVTMGLVDLVSMHTIMSVFVTSTVSMFGIALAVDYSLFILMRFREELRSGRQPQEAVDAAMATSGLAVVLSGMTVIASLTGIYLINTAALKSMATGAILAVAIAMLASITLTPAALATFGRAAVKRSVLMHWSQRSECTQSLFWTRWVGWVMHRS"
  },
  {
    "reference": "MSQRGDRGEGHARRPGRSSSFGGGHRGGGGVGGAGKGGGGSSGQPPLATNRSFRKSGNGHGGHQRAVSQPDTHGFQPAPAPTALQTPPLRPPAPQNAPAHVPVPAPRPQHHDPSGARAPTLPPSSENTANAPPLKGIPHAAPRAPSRISSTSTSQGAPKGGAYNLQFGSFPMNGGTGGSTMQFPARTSSAPPNLDEQKRMQALPEGHKVVPSGLVPQAPKHQQQQQPLQQQKQQPQSQPPLQQTRKDVVSSNHSSKPINPHIPSQVKSSVHVSPSVPNVAPPRPPVQQIPGMPMSMPFHHQAPLQFGGHNPQIPPQGVVPSSLQMSMGLHGANAPQVAQQMYIPTIQHHHQLQPPTMMHQAAGIPYGPAAHQLTQMSGMMNVGVAPQFTPQQPNKYVTGPTRKTTVKITHPDTHEELKLDKRMDSSGQRGLPSVQQQSQPVSTYGSPMGFYQQNSYNQSTMFYPTTSGVGQVPTVSQGPRFVSTQTVSYISPSMNTGPGSNKDNLAGSTTSGHSQVTGKPHPAGLHMEKSGVQTVTISAPPGKSDVNKLKPAEDVVSHRQKDNEAVSGVRKSGENESKASPITEKHPTPVSQPLQALAANPETTAAASFVVNSVPGDDGKSKESIQRTGSFKDSNKNATKDTRNLSQEPQSASSAEDLKVHTSVKDVCCGVSLMESKGVNKESEQTNAASASPTEMLKAADASSIDRSSARSTSESTENVQEVGKSDVAIGDSEKSGITNKVSPDLTKDDISSGSTGNESHEVCTLDL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.2. The protein should exhibit Basic and acidic residues, Polar residues compositional bias.3. The protein should be optimized for eukaryotic translation initiation factor 4F complex-specific folding and stability, while maintaining translation initiation factor activity, mRNA binding.4. The presence of MIF4G, MI domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "TAQGSLSLKAYRLTPKLMEVCKEKDFSPEALKKANITFEYMFEEVPIVIKNSHLINVLMWELEKKSAVADKHELLSLASSNHLGKNLQLLMDRVDEMSQDIVKYNTYMRNTSKQQQQKHQYQQRRQQENMQRQSRGEPPLPEEDLSKLFKPPQPPARMDSLLIAGQINTYCQNIKEFTAQNLGKLFMAQALQEYL",
    "response#2": "TAQGSLSLKAYRLTPKLMEVCKEKDFSPEALKKANITFEYMFEEVPIVIKNSHLINVLMWELEKKSAVADKHELLSLASSNHLGKNLQLLMDRVDEMSQDIVKYNTYMRNTSKQQQQKHQYQQRRQQENMQRQSRGEPPLPEEDLSKLFKPPQPPARMDSLLIAGQINTYCQNIKEFTAQNLGKLFMAQALQEYS",
    "response#3": "LQKMASHVLLATLSVPIQVSLSNTEKYLELDEVAREKSKRLANLLNLQNTPTRESLLQDMLKANVLQYVNTKVHCLYQLLEKDFRPLDLCAKVNEVCQYLESCDDADLCQYIKPIQNIAVTRLLKQVSQVFQTIEFSRLMALVPFMTEFQLERMIVDIAKEKNLQVRLDHRTKSISFGLDLI"
  },
  {
    "reference": "MTEWLISNQLIDYNCAVKSMEEKIQQIHNNSADELVWLLQHPPLYTAGISATADDIVEKLFPIYKTGRGGKHTYHGPGQRIIYLMLNLKKRNKCDIKLYIRDLSKWIINVLKQFNILGEFREDRIGIWVNHNGVEKKIAAFGIRLRKWVTYHGIALNVFPDLSHYKGIIPCGLQGYGVTSMEELGVKVPLSELDDILKKEFYKIF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm that is critical for protein lipoylation.2. Ideal protein should have a binding affinity for key enzymes in the protein lipoylation via endogenous pathway, Protein modification, protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 1/2 pathway to enhance the overall efficiency of the pathway.3. The protein should be designed to catalyze the following reaction L-lysyl-[protein] + octanoyl-[ACP] = H(+) + holo-[ACP] + N(6)-   octanoyl-L-lysyl-[protein].4. The protein must exhibit the following characteristics: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate- dependent enzymes; Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.. The designed protein sequence is ",
    "response#1": "MARMRVMVRERIDGVRPDTLWFLSHPPVYTVGKRTPPEHRPLAGLGIPVHETNRGGLLTYHAPGQLVGYVMCHIGAMNAVVPFLRLLEARLVDTVEALGIPAERRDTPPGSVELTGVWTRRTNRKIASIGLRCTRKVTSHGFALNVDCDMRPWTWATPCGMPEVEMTSVQRELTDAGHAVPSMAEVRELALLLGEQPDYSVAWEWQRSLVQQRHNCPELADVLILLEHPQVYTLGQGAKLDFLKFDPTQNKYELHRVERGGEVTYHCPGQLVGYPILNLKHYQQDLHWYLRQLEEVLIKVLDIFGLKGERYPGLTGVWLEGRKVGAIGIKVSRWITMHGFALNVSPNLEGFQRIVPCGISDKSVGSLAQFIPSITTDEVLPLVANAFRRACRAPVHWREGVRPDTLWFLSHPPVYTVGKRTPPEHRPLAGLGIPVHETNRGGLLTYHAPGQLVGYVMCHIGAMNAVVPFLRLLEARLVDTVEALGIPAERRDTPPGSVELTGVWTRRTNRKIASIGLRCTRKVTSHGFALNVDCDMRPWTWATPCGMPEVEMTSVQRELTDAGHAVPSMAEVREIAAEMLGARNAPHLRERGVEPTVYAQGLELQRQLVEARHSGRIGNTLLLLEHPPVLTLGRNSERKNVLASDEFLAYRGVEIHEVNRGGDVTYHGPGQLVGYPILDLRSFAESGERGRLGAVEYVRWVEEALIRTCADFGVQTQRVAGRTGVWTLPGGSVEEKKIAAIGVHISRGITSHGFALNVTTDLRDFDLIVPCGISDRKVTSLELEVIDEPALTMEKVIHSAARQ",
    "response#2": "MARMRVMVRERIDGVRPDTLWFLSHPPVYTVGKRTPPEHRPLAGLGIPVHETNRGGLLTYHAPGQLVGYVMCHIGAMNAVVPFLRLLEARLVDTVEALGIPAERRDTPPGSVELTGVWTRRTNRKIASIGLRCTRKVTSHGFALNVDCDMRPWTWATPCGMPEVEMTSVQRELTDAGHAVPSMAEVRELMARFGKVEEGKTPPTLRFYAWSPPTISLGYFQQLEKEIDLEAVKERGLGLVRRLTGGRAVLHDDEVTYSVVAREDHPLMIGGIRPSYLRLAKALAAGLRELGAPVEIASGRKGGREEHTTAACFDAPSWYEITCGGRKLVGSAQTRKGGVVLQHGSIVLTLNGDDLFAVLKMPSEAVRQRLLAKFYHQACGLEEVLGRRVEAGVIKENIVRAFTRLYAVEYSVAWEWQRSLVQQRHNCPELADVLILLEHPQVYTLGQGAKLDFLKFDPTQNKYELHRVERGGEVTYHCPGQLVGYPILNLKHYQQDLHWYLRQLEEVLIKVLDIFGLKGERYPGLTGVWLEGRKVGAIGIKVSRWITMHGFALNVSPNLEGFQRIVPCGISDKSVGSLAQFIPSITTDEVLPLVANAFAPTVRVEDISFEVLLDAKATGKKEDNQLFFCEHLPVLTIGKSGKDSNLLIPEETLRERGVSFYHINRGGDITYHGPGQITGYPVFDLEYWNLGLKQYIHMLEETIIRFLSLYDLKGERLEGATGVWLDPEVPGRARKICAIGVKSSRFVTMHGFALNINTDLSYFSLINPCGFTDKGVTSLAMELGVPQDFELAKSQL",
    "response#3": "MARMRVMVRERIDGVRPDTLWFLSHPPVYTVGKRTPPEHRPLAGLGIPVHETNRGGLLTYHAPGQLVGYVMCHIGAMNAVVPFLRLLEARLVDTVEALGIPAERRDTPPGSVELTGVWTRRTNRKIASIGLRCTRKVTSHGFALNVDCDMRPWTWATPCGMPEVEMTSVQRELTDAGHAVPSMAEVRELAAAADWHEYAQGLELQRQLVEARHSGRIGNTLLLLEHPPVLTLGRNSERKNVLASDEFLAYRGVEIHEVNRGGDVTYHGPGQLVGYPILDLRSFAESGERGRLGAVEYVRWVEEALIRTCADFGVQTQRVAGRTGVWTLPGGSVEEKKIAAIGVHISRGITSHGFALNVTTDLRDFDLIVPCGISDRKVTSLELEVIDEPALTMEKVIHSAARQLGYTGRQHGW"
  },
  {
    "reference": "MKVAVVGATGLVGTKMLQVLAERNFPVTELVPVASERSVGKEVEFKGKKYKVVSMTDGIAAKPAVALFSAGGTTSLEWAPKFAEAGIKVIDNSSAWRMDPKKKLVVPEVNADALTADDFIIANPNCSTIQMVVALQPLHTKYKIKRVVVSTYQSVTGTGAKAVEQLMGERERAVKGVSAEHAMAYKYQIDLNVIPQIDVFLENGYTKEEMKMVKETCKIMRDDSIRVTATTVRIPVMGGHSESVNVEFENDFDLEEVKSLLAAAPGIVVQNDDAAQFYPMPMDAHEKDEVFVGRLRRDESQPKTLNMWVVSDNLRKGAATNAVQIAEYMLSKNLL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.2. The protein with the reaction L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH, the Acyl-thioester intermediate must be optimized to ensure efficient catalysis.3. The presence of Semialdehyde dehydrogenase NAD-binding domains is critical for the interaction of the designed protein with its intended targets.4. The designed protein should have a binding site that is specific to the target phosphate, substrate, NADP(+) ligand.5. A protein with protein dimerization activity, NAD binding, NADP binding functions is required to enable isoleucine biosynthetic process, threonine biosynthetic process.6. A protein that is capable of catalyzing reactions within the Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5 pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MTVAILGATGAVGTRLIEQLEQSTIPVKSIKLLASSRSAGKILQFQGQNVVVEEATPESFTGVDLVLASGGGSVSARFAPEAIKRGAVVVDNSSQWRMNDNVPLVVPEVNEEALKNRPKGIIANPNCTTLSMMAALNPLHQKYGIERVFISTYQSVTGTGKKAVDQLFAESKGKYCDRIYPYPIYQNAIPQCDSFEENGYTKEELKLIREPKKIMGVKNMGVSATAVRVPVIGGHSESVQISFKKDFELDELREILKNTPGLILQDNPKNNYYPMPIHSYGKDEVFVGRLRRDLSQENSLLLWIVADNLRKGAALNTYEIAETLALYKAGLVEWMSTQTYQAASGGGAQHMRELLTQYGTLNAEVRPLLDDPKSAILEIDRKIIAKQRALTEAETANFGVPLGGSLIPWIDKDLGNGMSKEEWKGGAETNKILGQGEGFGTPAVPVDGFCVRIGAMRCHSQALTFKLRKDVPLADIEAMIAADNQWVKVVSNTREATIKDLTPVAVTGTLTIPVGRLRKMAMGPEYLGAFTIGDQLLEL",
    "response#2": "LVGLVGWRGMVGSVLMDRMQAEGDFALIEPVFFSTSNAGGKAPAMAKNETTLKDAYSIDELKRCDIIITAQGGDYTSDVFAKLRATGWSGHWIDAASTLRMKDDAVIILDPVNLPVIKNALAKGGKNWIGGNCTVSLMLMALYQKAKIKRIVASTYQSTSGAGKAAMDELYNQTKKIFTNEAKKPEIFPKQIAFNCIPHIGEFMEDGSTKEEWKMQEETKKILEADIKVTATCVRVPVFIGHAIAVNVEFYQHITEEQAREMLSEVEDTGILVYDRRKDGKYITQIDVVQENAVYVSRIRKDNTVEHGLNMWIVADNLRWGAAEPLRRMLRILLES",
    "response#3": "MTRVALLGATGMVGQRFVKLLENHEMFELSVLAASPSSAGKKYQDAAHWCIEGEVPEKFREMTVVKADLENVKKEDNVDFVFSALPSDVARIVEAEFAKAGIPVISDASAYRMEEDVPILIPEVNPDHLLAPIKAKYGLTRVIVSTYQAASGAGQSAWEELVAETTAHNAGQEMHANILPVKNAEHHYPLAYNLLPQIDVFEDDGYTHEEWKMIHETKKIMFGDKNSKAVKVTGTAVRVPVPIGHGEAVYFETEAGTGATSGDIQSILSKAPGVVLEDNPKEQLYPQPINAEGKRETFVGRVRPDLENDDSFHMWVVSDNLLDGVSAP"
  },
  {
    "reference": "MTFSKRIAGVLAILGAIVLSGCVGSGNAISISLGEAINLSQSNSFSKVAIDTGSGTMSMTVEAQTPIPITDSNGTAATASNGSVLTANIDSLNMADLKSLGFVFPVNTSTNAAASNLTSLLIYLGIPALFILMFIFFARRQAAGGDVAGFGRSGAKLLSPDRPKVTFADVAGVEEAKQDLQEVVEFLKDRAKFQAIGATIPKGVLLVGPPGTGKTLLARALAGEAGVPFFSISGSEFVELFVGVGAARVRSLFSEAKKMAPCIIFIDELDAVGRQRAGNIPGSHEEREQTLNQILVEMDGFDPNIGVIVLAATNRVDVLDQALLRPGRFDRRVTLDRPDTNGRIAILKIHSKGKALAKDVDLELIVKQTHGFSGADLAELMNEAAILAVRRNKKIIGMEELADSIDRVLAGPERKSLAIRPRDKELTAYHEAGHALVARNLPNIDPVLKISIVARGSMGGYTRFLEEDRYFITESQFRSTLATFMAGHAAEQMIFNEVSTGPHGDIKQATDIARKMITEYSMSDKLGFRTYGQTTVQGYLGIGPPETKDYSEDTARQIDDEVRVILENAHDTARKILSENRPRLDHLAGILLTKETLEGPELEKAFTEPITEKPAANPADSTGPTGQSQS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be able to bind Zn(2+) ligand in a variety of conditions2. The protein design must be able to perform ATP binding, zinc ion binding in order to contribute to protein catabolic process, cell division. The designed protein sequence is ",
    "response#1": "RNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPSSPATKNPCNIHYSPNDEDKGMVLPGTKCADGKACSNGRCVDNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPN",
    "response#2": "RNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPSSPATKNPCNIHYSPNDEDKGMVLPGTKCADGKACSNGRCVDRNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPSSPATKNPCNIHYSPNDEDKGMVLPGTKCADGKACSNGRCVDNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPGRNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPSSPATKNPCNIHYSPNDEDKGMVLPGTKCADGKACSNGRCVDRNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPSSPATKNPCNIHYSPNDEDKGMVLPGTKCADGKACSNGRCVDC",
    "response#3": "RNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPSSPATKNPCNIHYSPNDEDKGMVLPGTKCADGKACSNGRCVDRNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPSSPATKNPCNIHYSPNDEDKGMVLPGTKCADGKACSNGRCVDRNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPSSPATKNPCNIHYSPNDEDKGMVLPGTKCADGKACSNGRCVDRNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPSSPATKNPCNIHYSPNDEDKGMVLPGTKCADGKACSNGRCVDP"
  },
  {
    "reference": "MTKYVFVTGGVVSSLGKGITAASLGRLLKARGLKVVLQKLDPYINVDPGTMNPFQHGEVFVTDDGGETDLDLGHYERFTGEHLRRDSNVTTGSIYQTVIQKERRGDYLGDTVQVIPHITNEIKSRVRRLGEIDDVDVVIAEIGGTVGDIEGLPYLEAIRQLRKDVGRDNTLYIHVTLVPFIAASDELKSKPTQHSVAELRSRGIQPDVIVVRSDRSIDEQVRRKISLFCDVEPNAVISVPDANDIYEVPLMLRSAGLDDVVVDRLSIEAGEPDLTAWEALVAKSASVEGEVTVGVVGKYVDLPDAYLSVVEALRHAALANGVSLDLRWVPSDDLPGLLGSSYLEDLDGILVPGGFGGRGIEGKVFAVRHAREQRVPYLGLCLGLQCAVIEYARNVVGLNEAHSGEFDPTSPHRVIDLMDSQQDVNDLGGTMRLGLYAAKLADGSLVRKLYGEELIYERHRHRYEVNNRYRKDLEAAGLRLSGVSPDEQLVEVIEVPGHPFFLASQFHPEFKSRPDDPHPMFLGFVEAAIRYRKERLRPEPVAAEPATVELP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be optimized for activity in Pyrimidine metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The designed protein must have a Nucleophile; for glutamine hydrolysis active site that is highly conserved among related enzymes.3. The designed protein should have a binding site that is specific to the target Mg(2+) ligand.4. The designed protein must be able to perform ATP binding under specific conditions. The designed protein sequence is ",
    "response#1": "LPDAYLSVTEALRAGGFAEDARVTIRWVPSDECETPAGAAAQLSDVDAICIPGGFGIRGIEGKVGASRYAREHGIPALGLCLGLQCMVIDHARHVAGLEGASSAEFDPNTPAAVIATMEDQKEIVAGGGDLGGTMRLGAYPAALEPGSIVGELYGAQRVSERHRHRYEVNNEYREKLTGAGLVVSGTSPDGKLVEFIELPRDVHPYFVATQAHPELKSRPTRPHPLFRGLIR",
    "response#2": "LLDSYKSLIEALVHAGIHTNTTVELDFVDAEELETNGTAALANSHAVLVPGGFGQRGFEGKIRAAEYARQQNLPYLGICYGLHAAVIEYARNVVGLKGAHSTEVDPSAEHPVIGLVSEWMDLDGTVKDQSTNNGNLGGTMRLGAQACHLAEGSLVRETYQKPVVYERHRHRYEVNTKYVDAIEAAGMRISGINESDNLVEMIELPALDWFVACQFHPEFTSSPRDGHPLFNGFIATP",
    "response#3": "LPDAYISVTEALHHGAIANDAAVKIHWVNAENIEDPETDLDEVFVGCKGILVPGGFGDRGVEGKIKAIEYAREHKIPFLGLCLGMQCAVIEFARHVCGMEDAHSTEFNPETTHPVISLMESQQGIVKKGGTMRLGSYPCKITNGTHAKEAYGTNLIQERHRHRFEFNNEYRGALTEKGLVIAGTLPDDSLVEMIEVKDHPWFVATQAHPELKSRPNNPHPLFRDFVAATL"
  },
  {
    "reference": "MVHELTYSVFLEQVDAGQVMQVNKTGLDLTGQLNEKTTLITKDGTEQVVDKFYTRLLSDDPDLSQRILANNENAILVGERPKTSWWNHPLMFYLPFLLILLLWFFFLRQMQGGGNKAFSFGKSKAKMFNMDKPEVTFEDVAGCDEAKYELQEIIDFLRSPQKFQRLGGRIPKGALLVGSPGTGKTLLGRAVAGEAGVPFFSMSGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFESNEGVILLAATNRPDVLDPALLRPGRFDRQIVVDMPDLTGREAILKVHMKKVLVAEGVSARKIAAGTPGLAGADLENLVNEAALLAARKNHDKVTMDDFYDAREKVMMGPERRSMILSDDDKRITAYHEAGHTILSEVCEHANPTEKVTIIPRGQALGVTFTPPEKDQHHHSFEQMIDQICMALGGRVAEELFIGKIHTGAYQDIKMVTQIARAMVTSYGMSDAVGPLSLEEGNEQVFLGREYGRRTHVSEKTQELVDAEVKRIVEEQLTRARNLLEEHRTQMDTMAAALLERETLDREQIDLIMRGQELPEDDGLEVDHSTGDGPAPAAEESEEPKGADSPPEEDA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must have high specificity towards ATP hydrolysis activity.2. The protein should have a high affinity for Zn(2+). The designed protein sequence is ",
    "response#1": "LNKNILLKGPTGSGKTKLAETLSHVMNLPMHQVNCSVDLDTESLLGFKTIQTNEEGHQEIVFIDGPVIKAMKEGHILYIDEINMAKPETLPILNGVLDYRRQLTNPYTGEVIKAAPGFNVIAAINEGYVGTLPMNEALKNRFIVIEVDYIK",
    "response#2": "APKGILFVGPPGTGKTLLAKALAGEAEVPFHHVSGSDFVEMFVGLGASKVREMFAEVKKHTPAILFIDEIDAVGKKRGASSMGGNDEREQTLNQLLIEMDGFEKDTGVIVIAATNREDVLDTALLRRFGSKVQVPLPL",
    "response#3": "VGSPPWGAQRTWAKRLARGDSFSIIAPTGVGKTTFGAAASLFYACKKGMRSYIVLPTTTLAANVARKLESMVESTGCGRVRLLVIHSKLKTSERREAMERFEKGDFDILVTTAAFARKYADRLSGYRFRLVFVDDVDAVLRSARSVDAILKIVGFDEEAIEKGLEVLRLQREQARLVGLLQSQREEVREEARKKLLEVKRRLERLEAEIEARRGRTASLIVSSATGRPRGARVRLFRVLLNFEAGGRGDIA"
  },
  {
    "reference": "MGASEELEYLKSLVAQLNDKIASLEAKAKGPASKTPAQQLRTILIGPPGAGKGTQAPRIRDEFCVCHLATGDMLRDQVEKKTPLGIAAKKIMDAGGLVSDDIMVNMIKDQLENNEACKNGFVLDGFPRTVPQAQKLDGMLAERKEKLDSVVQLLIDDQLLISRITGRLIHPASGRSYHKIFNPPKKAGIDDLTGEPLIQRSDDNAETLTRRLKTYHTQTGPVVDYYKAKGLWHGVDAAQSPSVVWDSMRGIFAGRK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The AMP binding site should be stable and able to withstand changes in environmental conditions.2. For general function, the protein need meet that Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP3. The protein design should aim to mimic or enhance the function of natural proteins involved in phosphorylation, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "MKNRRVHLASGRTYHVSFNPPKTVDIDDETGEPLTQRER",
    "response#2": "MPIVL",
    "response#3": "GFSS"
  },
  {
    "reference": "MKHWIQENYKNRSWAGELNESQEGKQIVLFGWSFRFRDQGGVIFIDLRDRTGIIQVVARKELLGDSFTLAEKVRSEYVLAVRGTLKKRDLESINPRMQTGTIEVVLDQLEILNVAKTPPFSLDEFDEVSEELKLKYRYLDFRREELKNRMIKRHEFIFAIRNYLNKRKFVEIETPILNKSTPEGARDFLVPSRLNPNQFYALPQSPQIFKQILMVGGMERYFQIVKCFRDEDLRADRQPEFTQLDMEFSFVSQEEILSEIEGLVETIYKEVFNIQLSIPFPRMTYNTAMEEYGSDKPDLRFGMKLVDVSEIVKDCDFNVFAGAVKNGGTVKVVCVPGGSIISRKEIEDYTAWLNRDYKAKGLAYMKHGTEGLESTITKRFKKEELEAISKACGSKEGDMLFFGADEREIVNHSLGALRLKLSERFETPKENEINITWIVDFPMFEWNKDHKRWDALHHPFTSPSDESIPFFESMETLQKNAGNATAKAYDLVMNGVEIGGGSIRIHSREIQNKVFQILGINEEEAKEKFGFLLEALEFGAPPHGGLAFGIDRMLMLLTGGKSIRDVIAFPKTQKGLCLMSECPSPVEEKQLQELKIKLAKV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a high specificity for L-aspartate binding, with minimal off-target effects.2. The designed protein must be able to perform aspartate-tRNA ligase activity under specific conditions. The designed protein sequence is ",
    "response#1": "MPGTLPVINQTAVEYAVKAGMALGCDISKFSVFDRKNYFYPDLPKAYQISQLYYPLCLEGKVAIDTPNGQKYIRINHIHLEEDAGKLVHDDFNAVSLADYNRCGIPLVEIVTEPDISSAEEAKAFIEKVMLLLQYAGVSDCKMEEGSLRCDVNVSIMRPEDKELGTRAEIKNMNSLKSITRAINYEIKRQSRLLDAGKKVVQETRRFNENKGETSSMRSKENAHDYRYFPEPDILQVNFTDEMS",
    "response#2": "MPGTLPVINQTAVEYAVKAGMALGCDISKFSVFDRKNYFYPDLPKAYQISQLYYPLCLEGKVAIDTPNGQKYIRINHIHLEEDAGKLVHDDFNAVSLADYNRCGIPLVEIVTEPDISSAEEAKAFIEKVMLLLQYAGVSDCKMEEGSLRCDVNVSIMRPEDKELGTRAEIKNMNSLKSITRAINYEIKRQSRLLDAGKKVVQETRRFNENKGETSSMRSKENAHDYRYFPEPDILQVNFTDEMX",
    "response#3": "MPGTLPVINQTAVEYAVKAGMALGCDISKFSVFDRKNYFYPDLPKAYQISQLYYPLCLEGKVAIDTPNGQKYIRINHIHLEEDAGKLVHDDFNAVSLADYNRCGIPLVEIVTEPDISSAEEAKAFIEKVMLLLQYAGVSDCKMEEGSLRCDVNVSIMRPEDKELGTRAEIKNMNSLKSITRAINYEIKRQSRLLDAGKKVVQETRRFNENKGETSSMRSKENAHDYRYFPEPDILQVNFTDEMD"
  },
  {
    "reference": "MAEKPTNTSVPIPGSEDPQKENIRCLSTLGHFGFECLPTQLVNKSIQKGFSFNILCVGETGIGKTTLINTLFNTNLKETKSSHFYSKVGLTVKTYELLERNIPLRLTVVKTVGYGDQINKEASYQPVVDYLDAQFEAYLQEELKIKRSLADYHDSRIHVCLYFITPTGHSLKSLDLLTMKSIDRRVNIIPLIAKADSLSKNDLQRFKNNIMSELNSNGIQIYQFQVDDEASAQVNSSGLLPFAVVGSMEEVKVGKRMVRGRHYPWGVLQEVENENHCDFVKLRDLLLSTNMEDLKDQTHTQHYECYRSNRLQKLGFSDTGPDNRPVSFQEMYEAKRREFHNQCQKEEEELKQTFMQRVKEKELTFKDAEKELQDKFEHLKRIQQEEILKLEEERRKLEEQIIDFYKMKAASESAQAQVCTNIKKDKDRKK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be able to modulate protein localization to perinuclear region of cytoplasm in a way that leads to a desirable outcome.2. For general function, the protein need meet that Filament-forming cytoskeletal GTPase (Probable); Involved in the migration of cortical neurons and the formation of neuron leading processes during embryonic development The designed protein sequence is ",
    "response#1": "PRLHFFMVGFAPLTSRSSSSFRTISVPELTQQMFDSRNMMTAANYQNGRFLTCSTLFRGKVAMKEVEDQMRNMQNKYSSYFVEWIPNNVQTALCSMPPKGLKMAATFVGNSTSVQELFNRVW",
    "response#2": "MEFPFDIHKVLVEKITMLGPNLRPARRPFSARVDFQHQITMIVDEIGKASAKAQHLTAPITSASRLQGSDHRLYILKDCSAKNAGKGTVIGFLKVGYKKLFVLDHHGAHNEVEPLCVLDFYVHEALQRHGHGKELFQYMLQQEHLHPSQLAVDRPSEKLLSFLRKHYSLRSTIPQVNNFVVFEGFR",
    "response#3": "PRLHFFMVGFAPLTSRSSSSFRTISVPELTQQMFDSRNMMTAANYQNGRFLTCSTLFRGKVAMKEVEDQMRNMQNKYSSYFVEWIPNNVQTALCSMPPKGLKMAATFVGNSTSVQELFNRVW"
  },
  {
    "reference": "MIALVVSRSYTPPESVTIDKFWKMARDGLFTQITISDDYILAEPREAAQGVPRSRGIQKRYKVTMRVDNVDELAEKLRKACPDTVIQYEQSTQPLINTLLSIIPWLLIFGFIWFFVFRQLRSSAGGAGMLGSFGRSRHRVSNKELTNITFKDVAGVEEAKEEVAEIIEFLKNPKKFQRLGGRIPRGVLLVGEPGCGKTLLAKAIAGEADVPFFSISGADFVEMFVGVGASRVRDLFKSAKENAPCIIFLDEIDAVGRRRGAAFGGGGHDEREQTLNAILVEMDGFDTSDQVIVIAATNRDDILDPALTRPGRFDRRVYVPLPDIKGRLEILKVHAKKVKLAPSVDLMRIARGTPMFNGAELAATINEAAILATMQNKDMIEMDDLEEARDKVRYGRSNKSRQIEEKERENTAYHEAGHAVLQVLLKGEADPLHKVTIIPRGRALGATMSLPEKDRYGYARRWLMATLRVLCGGRIAEQRHSGDVSSGAAMDIEQVTHLSRAMVLEWGMSDKLGFVKYSPDNAREMLVPERNYSDETAGVIDAEIRRLSEEAFRDAERMISENWDKVVRVAEALLKYETLDAEEVESLCRGETLDRPSLTDLLADEGKAPRAARPVPPARPSTLPDTLPGGSLPQPGMG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a binding site that is specific to the target Zn(2+) ligand.2. The designed protein should have ATP hydrolysis activity, ATP-dependent peptidase activity, metalloendopeptidase activity, ATP binding within plasma membrane to regulate protein catabolic process, proteolysis.3. The designed protein have AAA+ ATPase domains with specific sequence motifs that are crucial for its activity.4. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.5. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.6. The protein must exhibit the following characteristics: Plays a role in the quality control of integral membrane proteins.; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. The designed protein sequence is ",
    "response#1": "MVMSEEEKLKTAYHESGHAIVGRSLEEHDPVYKVTIIPRGRALGVTMFLPEEDKYSYSHQSILDRICGLYGGRIAEELIYGEKGVTTGASNDIERATELAHNMVTKWGLSEKLGPIKYGDETDEPFLGRSAGASKQGISDDTAHEIDNEVKTILSNCYQHAKDILSENIEALHAMSKALMDYETLDVDQIDDSHAETDL",
    "response#2": "MVMSEEEKLKTAYHESGHAIVGRSLEEHDPVYKVTIIPRGRALGVTMFLPEEDKYSYSHQSILDRICGLYGGRIAEELIYGEKGVTTGASNDIERATELAHNMVTKWGLSEKLGPIKYGDETDEPFLGRSAGASKQGISDDTAHEIDNEVKTILSNCYQHAKDILSENIEALHAMSKALMDYETLDVDQIDDLRKTIAGYDEVVLVE",
    "response#3": "MVMSDEEKKLTAYHESGHAIVGLHMSEHDPVYKVTIIPRGRALGVTMFLPEQDRYSVSRCLLECQLAGLFGGRIAEELIFGKSRVTTGASNDIEKATDIARNMVTKWGLSPKLGPLTYREEEGEVFLGRSVTRHKDISESTSKEIDSEVRRIINSAYETAKRTLEKHVDQLHLMASALIKYETINEVQIKEYMKK"
  },
  {
    "reference": "MKLTCVVIVAVLFLTALADDSRNGLENRNEQERNENEMRDRRDCQDSGVVCGFPKPEPHCCSGWCLFVCA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that localizes to extracellular region and can perform toxin activity, ion channel inhibitor activity with high specificity and efficiency.2. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "MSFLGL",
    "response#2": "MPNS",
    "response#3": "MPIGVPKVS"
  },
  {
    "reference": "MADFHEFYPLRRHNTFGFDVRARYASHIRSEADLLAALNDPRAVGLPLVVLGGGSNVVLTGDLDALVLLMEIPGFRVGTAPDAWLVTAGAGENWHGLVCRTIAEGLPGLENLALIPGTVGAAPIQNIGAYGVELRERFASVRALDRQTMRFVDLDLEQCAFSYRDSLFKQAGRDRYIITAVTLRLSRDWQPVLAYGELAREVEGNAAPDAAAIRDAVIAIRSRKLPDPAQIGNAGSFFKNPLVSAEQRDVLLASHPDLVSYAQPDGSFKLAAGWLIDRCGFKGLNDGPVGVYGKQALVLVHHGGGTGAALLALAGRIADTVQARFGVRIEPEPVVL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein requires FAD to activate a critical catalytic site.2. For general function, the protein need meet that Cell wall formation.3. The protein contains novel FAD-binding PCMH-type domains that confer a unique function or activity.4. A protein with UDP-N-acetylmuramate dehydrogenase activity functions is required to enable regulation of cell shape, peptidoglycan biosynthetic process, cell wall organization, cell cycle.5. The design of the protein must focus on the precise arrangement of amino acids in the active site to enable effective catalysis of NADP(+) + UDP-N-acetyl-alpha-D-muramate = H(+) + NADPH + UDP-   N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine. The designed protein sequence is ",
    "response#1": "EAMFKPADAQDLADFLAGRPRDVAVRVIGVASNLLVRDGGVPGVVIRLGRAFTGVEVVGETLVCGASALDATVAKVAEAAGLAGLEFLSGIPGTLGGALRMNAGAHLREMADIVVLATAVDGLGQSHTLQVAHQRWMEEKLALRRKSQPLEFPNAGSVFLNPPGSLSAGQLIEEAGMKGFAIGGAQVSERHANFIVNRGGATAADVLALIDAVRARVLATCGIELQSEVRVYHR",
    "response#2": "MIGIGGSGMTGLAHLMVDLGFKVLGSDITDNTEIRQLRNRGVVISQGHEPEFLHANRPKLIVYSSAVRMDNPELAIASKLQIPIIRRAVLLAALAGKTGIAVAGMHGKTTTSAMIAHSLDILNPESGFAIGAAVPQLGRHARVSSSDDAFFVVETDESDGTLSEFSPEQSILLNIDEEHLDYYENLDAICTEFVAFGKQTKGTLIYCADDPRLTDLFGNRPKSISYGFNPTSDYQVEAGAKGEFQVKRREQVLGQFKIELLGEKNISNAVAAIALGHALVGGAADFLVFPRNRYELVRIVNFANQEDIPWMVLGNASNIIVRDGGIRGFVIMFDKLNNVAVDGYMIEAEAGANLIQTTHIALQNSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHVLVSCKVLTPQGQVKTLDVRDMKFGYRHSLVQETGDIVISAKFALSPVSRAICSIRSEKLPDPAVLGNAGSFFKNPLVSAAVVAQIKAQHPDLVAYAQPDGQMKLAAGWLIERAGWKGFREADAGVHKLQALVLVNYGAATGLQLLDLAQRIQKDIAERFNVELEMEPGADTQKVIGEIIAREG",
    "response#3": "VGGPAEAMFKPADAQDLADFLAGRPRDVAVRVIGVASNLLVRDGGVPGVVIRLGRAFTGVEVVGETLVCGASALDATVAKVAEAAGLAGLEFLSGIPGTLGGALRMNAGAHLREMADIVVLATAVDGLGQSHTLTPAQMGFSYRACALPEDWIFTGCVLAGRPGQTALLVADVAGTHGKTTTSSLVAQVLKECGLDPTVIVGGRLKAVGTGGVLGKGDYLVAEADESDGSFLKLSPTIAIVTNIDDDHLDYYKTFSNLEKAFADFLDRIPFYGVSLLCGDDPTLRKLMPRLKRRYETYGFGINCDLRASKVVTNAANTQFEVHYKAQKLGTLHLSLAGRHNILNALATVALTGHALGWDVEQVATGLAECPGVPRRFEVKFKGESNMVVDDYAHHPTEVKATLAAAKNSGWKRVIALFQPHRYSRTKLLLNDFAEAFHDAHEICLTEIYAASEKPLDGISGERLAEVVRESGHKAVFFEPRLDRLRSLVSRSLQDGDLILTMGAIAARMEALRQAREASQPLRARTGGSTFANPDPDLSGGRRAWELIDAAGCRGLRLGGAQVSEKHCNFLINTGEATAADLEALGETVRRRVMDTSGVALRWEIKRGWPDEQITERLKQFSEKRWASQPAASSAGCIFKNPGQGSAGKIIDELGLKNLAVGGARVSEVHANFIVNDGQATAQNVLDLIAQIQQKARDARGIDLETW"
  },
  {
    "reference": "MRVMEIRKNYQHLWRWGMMLLGILMICSAKENLWVTVYYGVPVWKEATTTLFCASDAQSYNTEVHNVWATHACVPTDPSPQEVVLENVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDLNNATNTTSANTTSGIEGGEMKNCSFNTTTGITDKPQNNYALFYTLDIVPIEGENNGFRVISCNTSIITQACPKVSFDPIPIHYCAPAGFAILKCNNKTFNGTGPCKNVSTVQCTHGIRPAVSTQLLLNGSLSEGEVEIRSENFTNNAKSILVQLNETLEINCTRPNNNTRRSITIGPGRAFYATDIIGDIRQAHCTISGEKWNNTLRQIVKKLREKFVNKIIAFNHSSGGDPEIVMHSFNCGGEFFYCNTTNLFSSTWYGNETEEPNNTTGNSTTITLPCRIKQIINMWQQVGKAMYAPPISGPISCSSNITGLILTRDGGNTNETTETFRPGGGNMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVQREKRAVGIGAVLLGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQSNLLRAIDAQQHMLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNTSWSNKSVEDIWQNMTWMEWEREIDNYTGLIYSLIEESQNQQEKNEQELLALDKWASLWNWFDITKWLWYIKIFIMIVGGLVGLRIVFTVLSIVNRARQGYSPLSFQTRLPAQRGPDRPDGIEEEGGERDRGRSAPLVDGFLAIIWVDLRSLFLFIYHRLRD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Transmembrane protein gp41: Acts as a class I viral fusion protein; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm..2. The protein design must feature the Di-leucine internalization motif, YXXL motif; contains endocytosis signal motif.3. The designed protein have Retroviral envelope protein GP41-like, Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains with specific sequence motifs that are crucial for its activity.4. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "MKVKGTRKNYQHLWKGGILLLGMLMICSATEKMWVTVYYGVPVWKEAKTTLFCASDAKAYSKEVHNVWATHACVPTDPNPQEMVLGNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCSNVTRHGNNSASNNTNVDNAXADIKNCSFNITTGIGNKMKKEYAFFNILDVTPIEGDNTSYILTSCNTSVITQACPKVNFDPIPIHYCAPAGFAILKCNDKEFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIVIKSSNITNNARNIIVQLNETVRINCTRPNNNTRKSIHIGPGSAWYTTGEIIGNIRQAHCNISEKDWEKTLRRVAIKLREQFNKTIIFNQSLGGDPEIVMHIFNCRGEFFYCNTTRLFNSTWKGNETATNNGTITLPCXIKQFINRWQGVGXAMYAPPISGNISCSSDITGLLLTRDGGYNNETDSEIFRPAGGDMKDNWRSELYKYKVVRIEPLGIAPSRAKRRVVQREKRAVGTLGAMFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLKAIEAQQHMLRLTVWGIKQLQARVLALERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNKTYSDIWDNMTWLQWDKEISNYTSIIYELIEESQNQQEKNEQDLLALDKWASLWNWFNITNWLWYIKIFIMIVGGLIGLRIVFAVLTIINRVRQGYSPLSFQTLTHHQREPDRPEGTEERGGEQDRDRSVRLVNG",
    "response#2": "MWVTVYYGVPVWRDADTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVKQMHKDIISLWDQSLQPCVKLTPLCVTLNCNRVNNSSSSNSTNNXXTSSNSTVSPGIKXEMMNCSFNMTXXVXXKKXKVSALFYRLDVXQIDSEQTNSTXYRLINCNTSTVTQACPKVTFEPIPIHYCAPAGFAILKCKDKNFTGTGPCXNVSTVQCTHGIRPVXTTQLLLNGSLAEERVMIRSENLTNNTXTILVQFQNPVRINCTRPSNNTRKSLRIGPGQTFYATGEVIGDIRRAHCNVSTQDWNDTLKEVVTQLRGHFKNKTIIFKNHAGGDLEIVTHSFNCGGEFFYCNTTDLFNSTWDKDGNGTVSSGSNGTITLQCRIKQIINMWQRVGQAMYAPPIQGVIRCDSNITGLLLTRDGGNGNDSQANETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVQREKRXLDRLXFPGELXKXXQRXVLGLXHXSXQGXXXNXXXETXRXLLALXEDY",
    "response#3": "MWVTVYYGVPVWRDADTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVKQMHKDIISLWDQSLQPCVKLTPLCVTLNCNRVNNSSSSNSTNNXXTSSNSTVSPGIKXEMMNCSFNMTXXVXXKKXKVSALFYRLDVXQIDSEQTNSTXYRLINCNTSTVTQACPKVTFEPIPIHYCAPAGFAILKCKDKNFTGTGPCXNVSTVQCTHGIRPVXTTQLLLNGSLAEERVMIRSENLTNNTXTILVQFQNPVRINCTRPSNNTRKSLRIGPGQTFYATGEVIGDIRRAHCNVSTQDWNDTLKEVVTQLRGHFKNKTIIFKNHAGGDLEIVTHSFNCGGEFFYCNTTDLFNSTWDKDGNGTVSSGSNGTITLQCRIKQIINMWQRVGQAMYAPPIQGVIRCDSNITGLLLTRDGGNGNDSQANETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVQREKRXXXDRXXAXXEESXXLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPSPQEVVLVNVTENFNMWKNNMVEQMHEDIIELWDQSLKPCVKLTPLCVTLNCTNYNANNSASNTEAVTLNCNNSASNNTNVDNAXADIKNCSFNITTGIGNKMKKEYAFFNILDVTPIEGDNTSYILTSCNTSVITQACPKVNFDPIPIHYCAPAGFAILKCNDKEFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIVIKSSNITNNARNIIVQLNETVRINCTRPNNNTRKSIHIGPGSAWYTTGEIIGNIRQAHCNISEKDWEKTLRRVAIKLREQFNKTIIFNQSLGGDPEIVMHIFNCRGEFFYCNTTRLFNSTWKGNETATNNGTITLPCXIKQFINRWQGVGXAMYAPPISGNISCSSDITGLLLTRDGGYNNETDSEIFRPAGGDMKDNWRSELYKYKVVRIEPLGIAPSRAKRRVVQREK"
  },
  {
    "reference": "MAMLLPQNPSQVHAAAVNGDKNTLHKLITESALRDSEDQFGRTPLMYCVLADRLDCAEVLLKAGAGINKTDHSQRTALHLAAQKGNVRFMKLLLSRHADWRLKDLEEMTPLHLASRHSSSKPLSLLLKHMAPGEVDTQDRNKQTALHWSAFYNHPEHVKLLIKHDSNIGIPDSEGKIPLHWAAHNKHPNATRTVRCILEAAPTESLLNWQDYEGRTPLHFAVADGNEAVVEVLTSYEGCSVTAYDNLFRTPLHWAALLGHAKIVHLLLERNKSGMIPSDSQGATPLHYGAQSNFADTVAVFLKHHSVRDEPDLEGRTAFMWAAGKGSNDVIKIMLDLKKDIDINMTDKYGGTALHAAALSGHVSTVRLLLEQGGMVDPLDVMKHTPLFRACEMGHRDVILTLIKGGARVDLVDIDGHSALHWAALGGNAEVCEVLMENGISPNLQDQAGRTPLQCAAYAGYINCMALLIQHDADPNIQDKEGRTALHWSCNNGYLDAVKLLLGCGAFPNHMEHTEERYTPLDYALLGEHQELTQFLLEHGALSIAAIQDIAASSIQALYKGYKVRRAFRERKKLLMRHEQLRKDAAKKREEERRREAEQQLSFAEAGQKQRVLLAAVGVEKLSLDEAEQRVKDSVAAKGHKHKKSSSAHNSQSRREKPSRAERRTREPETSDAPSIRSLPPVTPMSTKKCPVTREEVCVRETFGPDTGISLNCGSANERRSPAGSSRPGSAKPVHTGTHVAPGHMETTPTPKPRPSTTGAHSKNASQD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein contains novel IQ domains that confer a unique function or activity.2. Target a Polar residues, Basic and acidic residues compositional bias in the protein's composition for improved properties.3. The designed protein should have calmodulin binding within cilium, cytoplasm to regulate heart looping, establishment of planar polarity, pronephros morphogenesis, pronephros formation, body morphogenesis.4. For general function, the protein need meet that Required for normal renal development and establishment of left-right axis; Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled for degradation by the ubiquitin-proteasome; Probably acts as a molecular switch between different Wnt signaling pathways; This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling.5. The protein design should include a D-box motif to ensure proper function. The designed protein sequence is ",
    "response#1": "MYFAPEELNFARIWIGIWSVLCCASTLFTVLTYLVDMKRFSYPERPIIFLSGCYTMVAIAYIAGFLLEDKVVCNERFAEDGYKTVAQGTKKEGCTFLFMMLYFFSMASSIWWVILSLTWFLAAGMKWGHEAIEANSQYFHLAAWAVPAIKTITILAVGQVDGDTLSGVCFVGINNVDALRGFVLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEKLEKLMVRIGIFSVLYTVPATIVIACYFYEQAFREQWEKSWISQSCKTYAIPCPSTGHPPMSPDFTVFMIKYLMTLIVGITSGFWIWSGKTLNSWRKFYTRLTNNRLVSTASALVNANVSFECRLNFGTDVAYLWNFGDDTIELGSSSSSHVYSREGEFTVEVLAFNNVSSTTLRKQLFIVRPGTIRVFNFTTGEFERFHPNMLCEEICNTMCRQLGIAPIAQLLYGIREHSTSRRPSPLVRLDLTWCLPGERLNCQLVYCFRMRFRVPELDSQLELIDGRSHKFLYRQMRYDMRTEQIPEIRYPEHKDKSTGLAVMDMLIDDQEQSEDQQAMRSIEKLYKLYLPPSLWRAHSFFVGSKIREVFRSLKANSLSVERLKWHYVHQVSHLAPTYMTEQFTCTVQYLPNEEVARGSGPIGTSLAHSTSTLASSGSTNTLSTLTTNTNSVALGGSGKKAKRRSTSGGIDVYVRVFPHDSLEPGLKVARVTSEATLKWILVGAVEGIFMISKINDTSVRLEIVGLPKGYEMQFQTEKEMKSFISYLGIYIRLSSKW",
    "response#2": "GTIRVFNFTTGEFERFHPNMLCEEICNTMCRQLGIAPIAQLLYGIREHSTSRRPSPLVRLDLTWCLPGERLNCQLVYCFRMRFRVPELDSQLELIDGRSHKFLYRQMRYDMRTEQIPEIRYPEHKDKSTGLAVMDMLIDDQEQSEDQQAMRSIEKLYKLYLPPSLWRAHSFFVGSKIREVFRSLKANSLSVERLKWHYVHQVSHLAPTYMTEQFTCTVQYLPNEEVARGSGPIGTSLAHSTSTLASSGSTNTLSTLTTNTNSVALGGSGKKAKRRSTSGGIDVYVRVFPHDSLEPGLKVARVTSEATLKWILVGAVEGIFMISKINDTSVRLEIVGLPKGYEMQFQTEKEMKSFISYLGIYIRLSSKWNRLVSTASALVNANVSFECRLNFGTDVAYLWNFGDDTIELGSSSSSHVYSREGEFTVEVLAFNNVSSTTLRKQLFIVRK",
    "response#3": "MGETKIIYHLDGQETPYLVKLPLPAERVTLADFKGVLQRPSYKFFFKSMDDDFGVVKEEISDDNAKLPCFNGRVVSWLVSAL"
  },
  {
    "reference": "MVRRVQPDRKQLPLVLLRLLCLLPTGLPVRSVDFNRGTDNITVRQGDTAILRCVVEDKNSKVAWLNRSGIIFAGHDKWSLDPRVELEKRHSLEYSLRIQKVDVYDEGSYTCSVQTQHEPKTSQVYLIVQVPPKISNISSDVTVNEGSNVTLVCMANGRPEPVITWRHLTPTGREFEGEEEYLEILGITREQSGKYECKAANEVSSADVKQVKVTVNYPPTITESKSNEATTGRQASLKCEASAVPAPDFEWYRDDTRINSANGLEIKSTEGQSSLTVTNVTEEHYGNYTCVAANKLGVTNASLVLFRPGSVRGINGSISLAVPLWLLAASLLCLLSKC",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must have at least one Ig-like C2-type domain.2. For general function, the protein need meet that Essential for normal growth of the hippocampal mossy fiber projection .; Contributes to the guidance of developing axons and remodeling of mature circuits in the limbic system3. The protein should be designed to localize specifically to cytosol, plasma membrane, extracellular region in order to perform its role in cell adhesion more effectively. The designed protein sequence is ",
    "response#1": "HRFQSCAYRSNQWRYRGRCDSIQFAVDKRVFIAGFGLYGSSCGSAEYSAKIELKRQGVVLGQNLSKYFSDGSSNTFPVWFEYPVQIEPDTFYTASVILDGNELSYFGQEGMTEVQCGKVTVQFQCSSDSTNGTGVQGGQIPELIFC",
    "response#2": "HRFQSCAYRSNQWRYRGRCDSIQFAVDKRVFIAGFGLYGSSCGSAEYSAKIELKRQGVVLGQNLSKYFSDGSSNTFPVWFEYPVQIEPDTFYTASVILDGNELSYFGQEGMTEVQCGKVTVQFQCSSDSTNGTGVQGGQIPELIFC",
    "response#3": "HRFQSCAYRSNQWRYRGRCDSIQFAVDKRVFIAGFGLYGSSCGSAEYSAKIELKRQGVVLGQNLSKYFSDGSSNTFPVWFEYPVQIEPDTFYTASVILDGNELSYFGQEGMTEVQCGKVTVQFQCSSDSTNGTGVQGGQIPELIFC"
  },
  {
    "reference": "MPMTLGYWDIRGLAHAIRLLLEYTGSSYEEKRYTMGDAPDYDRSQWLSEKFKLGLDFPNLPYLIDGSHKITQSNAILRYIARKHNLCGETEEEKIRVDILENQAMDVSNQLARVCYSPDFEKLKVEYLEQLPGMVKLFSQFLGQRTWFVGEKITFVDFLAYDILDLHLIFEPTCLDAFPNLKDFVARFEVLKRISAYMKTSRFLRTPLYTKVATWGNK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a binding site that is specific to the target glutathione, substrate ligand.2. The designed protein should have leukotriene-C4 synthase activity within cytosol to regulate nitrobenzene metabolic process, glutathione metabolic process.3. For general function, the protein need meet that Can also catalyze the transfer of a glutathionyl group from glutathione (GSH) to 13(S),14(S)-epoxy-docosahexaenoic acid to form maresin conjugate in tissue regeneration 1 (MCTR1), a bioactive lipid mediator that possess potent anti-inflammatory and proresolving actions. The designed protein sequence is ",
    "response#1": "MDVSNQLARVCYSPDFEKLKPEYLEELPTMMQHFSQFLGKRPWFVGDKITFVDFLAYDVLDLHRIFEPNCLDAFPNLKDFISRFEGR",
    "response#2": "MDVSNQLARVCYSPDFEKLKPEYLEELPTMMQHFSQFLGKRPWFVGDKITFVDFLAYDVLDLHRIFEPNCLDAFPNLKDFISRFEGR",
    "response#3": "VMDVRLAMARICYSPDFEKLKPGFLKEIPEKIKLFSEFLGKRPWFAGDKLTYVDFLVYDVLDMHRIFEPKCLDAFPNLKDFISRFEV"
  },
  {
    "reference": "MDSPYLEIEVPLSSPIHAFDFPQWHVPLKAGACAPRMTEHGEEIPGLMDGCLDLSITKHTVMMWLAALLLMATLFIWGNRDKTKLVPRGAGANILEMLVLFVRDELAIKNIGKEEGPRYVPFLLTVFFFILFMNMLGMVPWMATATGNLAVTMALALCTFVITQVAGIRAAGLGGYLKHLTGGVAPWLWPIMVPVEVLGLFTKPFALTMRLFANMLAGHIVYFFLLGLIFLLGHPAVAAVSVPFAFAIFLLELFVAFVQAYVFAMLSALFIGMSVAMGHHHDDHGHDHPEAGPSHDQGKAHHA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Key component of the proton channel; it plays a direct role in the translocation of protons across the membrane..2. The protein should be optimized for plasma membrane, proton-transporting ATP synthase complex, coupling factor F(o)-specific folding and stability, while maintaining proton-transporting ATP synthase activity, rotational mechanism.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "MPVPPRY",
    "response#2": "GLLPRF",
    "response#3": "GYAI"
  },
  {
    "reference": "MGYKIAVVGATGNVGREMLNTLEERQFPADEIFALASSRSVGRELDYGDKEIKVVDAATFDFSKVDIALFSAGGTTAKTLAPKAAAAGCVVIDNSSYWRMEPGVPLVVPEVNGAALAGYAKKNIIANPNCSTIQMVMALKPLHDLQPIKRVVVATYQSVSGAGRLAMDELFNQTKGIYVNDLPVKEQFTKQIAFNVIPHIDEFMDDGATREEWKMTAETRKILDPDIAVHATCVRVPVFVGHAEAINIEFEQPVTVEEARKAMKAFPGVSVVDYRQDEGYVTPQEVQGEDNVFVSRVRKDPTVENGLSLWCVGDNLRKGAALNAVQIAEMLVAEYLPKK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The presence of Semialdehyde dehydrogenase NAD-binding domains is critical for the interaction of the designed protein with its intended targets.2. The Proton acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..4. A protein that can modulate the overall flux of metabolites through the Amino-acid biosynthesis, L-threonine biosynthesis, L-threonine from L-aspartate: step 2/5 pathway, enhancing or suppressing its activity as needed.5. The protein should have a high affinity for substrate, NADP(+), phosphate.6. The designed protein should have aspartate-semialdehyde dehydrogenase activity, NAD binding, NADP binding to facilitate isoleucine biosynthetic process, threonine biosynthetic process, diaminopimelate biosynthetic process, lysine biosynthetic process via diaminopimelate, 'de novo' L-methionine biosynthetic process. The designed protein sequence is ",
    "response#1": "MAVAIVGATGLVGRELLRLLEQNDFPLAGLRLLASSRSAGSRLPFRGEIIPVEETTPSSFRGIDLAFFSATSAVSRGLVPHAVEAGAVVIDDSKAWRLEPHVPLVVPEVNGDTLASARRFVSGICVRVPVFTGHLAPIHRKYGIERINVATYQSVSGAGRSGLEELGRQTAQLLAFQEIEPKKFQAQIAFNLIPHIDEFLPNGYTKEEMKLVWETRKILEDDSIQVNPTAVRVPVFYGHSEAVNIETKEKITPEQARELLRAAPGVEVVDEHKAGGYPTPVTHASGRDPVFVGRIREDFSHPRGLNLWVVADNIRGARGALKPLHEAAGLQRLVVSSYQAVSGSGVAGVDELANGVAAAGDKARELAYDGSAITFPEPDKYVAPIAHNVIPLAGSIVEDGLNETDEEQKLRNESRKILGLPDLLVSGICVRVPVFTGHSLAINAEFEQAMTPERAREILAEAPGVELVDVPTPLQAAGKDPSYVGRLRQDPGVPGDRGLALFISNDNLRKGVAVTPAVEG",
    "response#2": "MSNLAVVGATGAVGRIVLEQLALRKLPINRLKLLASQRSVGKEVRFADETLKVQLLEPDAFQGIDIVVASTPDEVSAEFASWATDAGAVVVDESGYWRMDPKVPLVIPEVNPEAIHDLIAKGVKGYIGGNCTVSCMLMGLKPLHDLATIKRVVVATYQSVSGAGKEGVDELWDQTKGVFVNDKPEPRKFTKQIAFNVIPHIDVFMEDGYTKEEWKMVAETKKILDPKIKLTATCVRVPVFVGHSEAVNLEFANPISADEAREALREAPGISVIDKREAGGYATPVEAAGEYDTYVSRIREDVTVENGLSLWVVSDNLRWGAAEPLRRMLKIILEQ",
    "response#3": "LGGLLEHDLVEWISPMTYQAASGSGAQHMRELLRQMGHLHAAAGTRLADPAGAILDIDRDVSEALRSAEYPADQFGVPLAGSLIPWIDRAMDSGQSREEWKAGVETNKILGRRTNPLPIDGLCVRIGAMRSHAQALTIKLRRDVPMADIETLLAEANDWVHVVPNEPAASQQQLTPAAVSGGLDIPVGRLRKLAMGDDYLSAFTVGDQLLDAQARHLWPLHQRNPVRRIIVDTYQSVSGTGWAAMEELRQQVRSILEGEECTPQVYSHVIAFNVFPHVEDFSDDGYTKEELTIIHETRKIMGDPQIAISATCVRVPVMVGHSLAVHVEFAHPFLPEEAREVLREAPGVKVVDDPSHALYPTPLMAAGGDYALVGRIRRDASSERGLALWISCDNLRLPGLSRLWPLHQRNPVRRIIVDTYQSVSGTGWAAMEELRQQVRSILEGEECTPQVYSHVIAFNVFPHVEDFSDDGYTKEELTIIHETRKIMGDPQIAISATCVRVPVMVGHSLAVHVEFAHPFLPEEAREVLREAPGVKVVDDPSHALYPTPLMAAGGDYALVGRIRRDASSERGLALWISCDNLRV"
  },
  {
    "reference": "MARTEGDTWDIVTSVGATALVVSAMRAIEARKPEPLARDDYAQHFVAATKAEAPLFSELLEDPEAAQAPDIQLFSSYLGARTKYFDEFFLTAGNAGVRQAVILAAGLDVRGYRLPWAAGTTVYELDLPKVLEFKKQVLDEHNARATATVLDLHVDLRDDWPTVLKAAGFDPAQPTAWLAEGLLPFLPGAAQDLLFERIADLSAPGSRVAVEDFGAPGNQADRMSNAMQNEEGALQRIFKSIVEEDAPPSSLWFGDEREDPARWLTGHGWTVEATTAGELLKRYNRVPLAGEHELTDAMGQSRYFTAVLGA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a high affinity for S-adenosyl-L-methionine. The designed protein sequence is ",
    "response#1": "TGCPLRCVYCDTAYAFTGGERLSLSSILDQVAAYHTQDVCVTGGEPLAQPACLPLLTSLCDAGHLVSLETSGALL",
    "response#2": "SAGWPTVFVRLTGCPLRCVYCDTAYAFTGGERLSLSSILDQVAAYHTQDVCVTGGEPLAQPACLPLLTSLCDAGHLVSLETSGALSIEGVDPRVRVVMDLKTPSSGEAEKNRFENIDLLKTSDEIKFVIGSAEDFKWAVQHVRQFQMDKRFNVLISPVFGEVTPLALADWILESGLRLRFQQQLHKLIWGA",
    "response#3": "TGCPLRCVYCDTAYAFTGGERLSLSSILDQVAAYHTQDVCVTGGEPLAQPACLPLLTSLCDAGHLVSLETSGALN"
  },
  {
    "reference": "MLQIGEDVDYLLIPREVRLAGGVWRVISKPATKEAEFRERLIQFLQEEGRTLEDVARIIEKSTPHPPQPRKRTKELRVRRVPQMVTPPLRLVVGTYDSSNGSDSELSDFDTSKVKGNRSSSGRTRKVRKMPVSYLGSKFLGSDVESEDDQELVEAFLRRGEKPSAPPPRRRVNLPVPMFENNLGPQPSKADRWREYVSQVSWGKLKQRVKGWAPRSGSEVGQTQQASTAAERAGEMRHSQASSDDDSSRNTGDRSDQMLGTRRWKPKIKWVSLRRCRKEQVPPFAQGTGMPAEEHPEAAENQGAEAAANQRAEPLASPRAEAAASPRAETAADPRVEAVASPRAEAAASPRAEAVADPRAEAAASPRAEAAASPRTEAAASLRAEAVASPRAEAAASPRAEAAADPRAEAAASPIAEAAANQKAELVDSPRAETAADPRAEAAASPRAEAVADPRVEAAASPIAEAAANQKAELVDSPRAETAADPRAEAAASPRAEAAADPRAEVAASPRAEAAASPRAEAEASPRAEAAASPKAEAEANLRVEAAAYLRAGVPPDQRAEAIDSQRAEGPANQRTGATENQRVEVLADQRAGVLHDQREEAGPQGIQEASAGSGSRAQKQVKTVRFQTPGRFSWFRMRRRVFWHTPRLPTLPRRVPRAGEARSLRVLRADIRADVEHREQEEQL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Target a Basic and acidic residues, Polar residues compositional bias in the protein's composition for improved properties.2. For general function, the protein need meet that Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity; It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer3. The protein should be optimized for stability and expression in centrosome, nucleoplasm, 3M complex where it will participate in microtubule cytoskeleton organization, regulation of mitotic nuclear division. The designed protein sequence is ",
    "response#1": "VCMVGPPGTGKTMMAKAVSYECKTTFFCVSCGDLASKWRGDAEKMVKLLFEMARFHAPSTIFIDEIDTIGSQRGGANEHEASRRLKAQLLVEMDGFAGEDSDKRVLILAATNFPWNLDEALRRRFEKRIYIPHFLMTSYTPLRQVNQISSRTSAIDVMKRLIQPQNIMARTRLKEGKYISMCDFFQGDVSYDEINEALQRFTDRRLAEFVPWNKEAIKVVHTRVSPLVKRGNRMSGMLLANNTSIRYYFQDII",
    "response#2": "LYNSLNSLMIHLCPSRFSHFLMTSYTPLRQVNQISSRTSAIDVMKRLIQPQNIMARTRLKEGKYISMCDFFQGDVSYDEINEALQRFTDRRLAEFVPWNKEAIKVVHTRVSPLVKRGNRMSGMLLANNTSIRYYFQDILN",
    "response#3": "LYNSLNSLMIHLCPSRFSHFLMTSYTPLRQVNQISSRTSAIDVMKRLIQPQNIMARTRLKEGKYISMCDFFQGDVSYDEINEALQRFTDRRLAEFVPWNKEAIKVVHTRVSPLVKRGNRMSGMLLANNTSIRYYFQDILK"
  },
  {
    "reference": "MEKEIKDHKSVGIVEKQFFTCAQVPSPLILENGAKLGPITIAYETYGNLSARKDNAILINHAFSGDSHVAGHYATDGPKEKPGWWDFLVGPGKGIDTDKYFIICSNILGSCNGTTGPASKNSETGEAYALDFPMVTIGDMVATQKLLIDHLGIPKLLAVIGGSVGGMQTLEWAIRYPEMMHSVIPIATTMRHSALAIAFNEIARQAIMTDPHWNRGKYYGQAHPDTGLAVARMVGHVTYLSDEAMRRKFGRNLQEKENLSYGFDADFQVESYLRYQGNKFVHRFDANSLLYITKASDYFDIVERISTSGPETELTAPQQKYLVISYSSDWLYPTYQARDLVKALKRSGRNVSFSEIESDCGHDAFLIPDDRLEQLMRGFLAGIYTGIAEI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that is capable of catalyzing reactions within the Amino-acid biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "LTRFDHVVGNVPELAPAAAYIAGFTGFHEFAEFTAEDVGTTESGLNSVVLANNSEGVLLPLNEPVHGTKRRSQIQTFLEHHGGPGVQHIAVASSDVLRTLRKMRARSAMGGFDFLPPPLPKYYEGVRRLAGDVLSEAQIKECQELGVLVDRDDQGVLLQIFTKPVGDRPTLFLEMIQRIGCMEKDERGEEYQKGGCGGFGKGNFSELFKSIEC",
    "response#2": "LTRFDHVVGNVPELAPAAAYIAGFTGFHEFAEFTAEDVGTTESGLNSVVLANNSEGVLLPLNEPVHGTKRRSQIQTFLEHHGGPGVQHIAVASSDVLRTLRKMRARSAMGGFDFLPPPLPKYYEGVRRLAGDVLSEAQIKECQELGVLVDRDDQGVLLQIFTKPVGDRPTLFLEMIQRIGCMEKDERGEEYQKGGCGGFGKGNFSELFKSIEC",
    "response#3": "LTRFDHVVGNVPELAPAAAYIAGFTGFHEFAEFTAEDVGTTESGLNSVVLANNSEGVLLPLNEPVHGTKRRSQIQTFLEHHGGPGVQHIAVASSDVLRTLRKMRARSAMGGFDFLPPPLPKYYEGVRRLAGDVLSEAQIKECQELGVLVDRDDQGVLLQIFTKPVGDRPTLFLEMIQRIGCMEKDERGEEYQKGGCGGFGKGNFSELFKSIEC"
  },
  {
    "reference": "MPLAKRIIPCLDVDKGRVVKGVQFVDIRDAGDPVEVAKKYNEQGADEITFLDITASHEARETTINTVEKIAAEVFIPLTVGGGIRTLDDIRNMLNAGADKVSINSAAVKDPDFVRIAAEKFGSQCIVVAIDAKRVSADGEPGRWEIFTHGGRNPTGIDAVEWAVRMTEYGAGEILLTSMDRDGTKDGFDLGVTRAISEAVRVPVIASGGVGNLQHLVDGVIKGGADAVLAASIFHFGEYTVPEAKAYMEQHGIEVRL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit..2. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "MQGYMDRASAEATLASGQVTFFSRSKNRLWRKGETSGNTLDLVELTADCDRDALLALVRPQGPTCHLGSTSCWN",
    "response#2": "MQGYMDRASAEATLASGQVTFFSRSKNRLWRKGETSGNTLDLVELTADCDRDALLALVRPQGPTCHLGSTSCWK",
    "response#3": "MQGYMDRASAEATLASGQVTFFSRSKNRLWRKGETSGNTLDLVELTADCDRDALLALVRPQGPTCHLGSTSCWR"
  },
  {
    "reference": "ADNKRPIWVMGGMVNSLAQIKEFVGLGLDNSEKDNKWYKQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be able to bind Mg(2+) ligand in a variety of conditions2. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.3. The ideal protein would be one that plays a critical role in hemolysis in another organism through toxin activity, lyase activity, phosphoric diester hydrolase activity within extracellular region.4. The protein must exhibit the following characteristics: In vivo, intradermal injection induces dermonecrosis; Induces, hemolysis, vascular permeability, edema, inflammatory response, and platelet aggregation .. The designed protein sequence is ",
    "response#1": "MKKKFLKGLCCAFVISITCLGASSKAYGWDGKKDGTGTHSMIVTQAVKVLENDMSKDEPEIVKQNFKILQDNMHKFQLGSTYPDYDPNAYKLFQDHFWDPDTDHNFSKDNLWYLSYSIKDTAESQVRKFTALARNEWEKGNYEKATWYFGQAMHYFGDLNTPYHAANVTAVDSIGHTKYEGFAEKRKDQYRINTTGIKTNEGFYADALKNSNFDSWSKEYCKGWAKQAKNLYYSHSTMKHTNEDWDYSASHALKNAQMGTAGCIYRFLYDVSKDLLPV",
    "response#2": "WDGKKDGTGTHSMIVTQAVKVLENDMSKDEPEIVKQNFKILQDNMHKFQLGSTYPDYDPNAYKLFQDHFWDPDTDHNFSKDNLWYLSYSIKDTAESQVRKFTALARNEWEKGNYEKATWYFGQAMHYFGDLNTPYHAANVTAVDSIGHTKYEGFAEKRKDQYRINTTGIKTNEGFYADALKNSNFDSWSKEYCKGWAKQAKNLYYSHSTMKHTNEDWDYSASHALKNAQMGTAGCIYRFLYR",
    "response#3": "WDGKKDGTGTHSMIVTQAVKVLENDMSKDEPEIVKQNFKILQDNMHKFQLGSTYPDYDPNAYKLFQDHFWDPDTDHNFSKDNLWYLSYSIKDTAESQVRKFTALARNEWEKGNYEKATWYFGQAMHYFGDLNTPYHAANVTAVDSIGHTKYEGFAEKRKDQYRINTTGIKTNEGFYADALKNSNFDSWSKEYCKGWAKQAKNLYYSHSTMKHTNEDWDYSASHALKNAQMGTAGCIYRFLYN"
  },
  {
    "reference": "MSHSATHISVHKRPQLKRLVLLTGFIVASGSLLPACGKVAQDNNAKPTAESSNSGITDSRDMLPSDVLNQLKELPQLTEGLGQNGAAVLDTNKPTLVKFWASWCPLCLGTLEETETWRKDAAFAGLNVLTVASPGHLNEKETTDFTQWYAGVQADYPKLPVLMDSSGSLIKQLGVQVYPSWAIFDKQGNLVRLIKGNLSEEQAYALAQNAGNDFAALKNSSAKPTQTGAASTANAKADSPNNKNGVYYNSDGAPINTKTIYLAGGCFWGLEAYMERVDGVVDAISGYANGNPSLTDPSYEQVIAGSGHAETVKVVYDTDKIDLETVLKYYLRVIDPTSLNKQGNDRGVQYRTGIYYTDAADKEVINKALQSIESKYTKPIVVEHQPLKNFSLAEEYHQDYLAKNPNGYCHIDLSMADDKLAPTAKAPKLAPATTVAEALNPKRYANFDKNALKNTLTKAQYNITQKAGTERAFSHEYDHLFAPGIYVDVVSGEPLFLSTHKYDSGCGWPSFWQPIAPEVITEHKDTAYNMVRTEVRSRVADSHLGHVFNDGPKDKTGLRYCINGGALQFIPVDKMAQSGYGVFVDQVALKPVK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be optimized for peptide-methionine (R)-S-oxide reductase activity activity in order to improve response to oxidative stress, protein repair, protein modification process.2. The designed protein must have at least one MsrB domain.3. A key design requirement for the protein is the optimization of the active site to achieve high catalytic efficiency for [thioredoxin]-disulfide + H2O + L-methionyl-[protein] =   [thioredoxin]-dithiol + L-methionyl-(S)-S-oxide-[protein].4. For general function, the protein need meet that Has an important function as a repair enzyme for proteins that have been inactivated by oxidation The designed protein sequence is ",
    "response#1": "PSQEEILDTLTTEQFRVTQQKDTERPFSSEYDTLDEPGIYVDIVTGQPLFSSDDKYDAGCGWPSFTRPMVGDALHYEEDFSHGMHRVEVTSSRGESHLGHVFPDGPHAEGGQRYCINGASLRFVPLAQMEAEGE",
    "response#2": "MKPSREELKKRLTPLQYDVTQEEGTEPPFRNEYWDEKREGLYVDIVSGEPLFSSRDKYDSGTGWPSFTRPVDPQGIVERVDRKLFTTRTEVRSRNGDSHLGHVFPDGPQPTGLRYCINSAALRFVPA",
    "response#3": "HKPSDEEIKKMLTAEEYAVTQKNDTERAFSNRYWDKFDEGIYVDIVTGEPLFSSKDKFDSGCGWPSFSKPISPDVATYKDDHSFNMVRTEVRSRVGDSHLGHVFTDGPKDKGGLRYCINSLSITFIPR"
  },
  {
    "reference": "MKFDMLWSKIHRATVTDANLNYVGSITIDEELMEAAELLVGQKVEILDVNNGERFSTYVIRGERGSREICLNGAAARKVAIGDKIIIVAYAQYDRSELSSYKPTVVLVDEKNDIVQIKHEV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Catalyzes the pyruvoyl-dependent decarboxylation of aspartate to produce beta-alanine..2. The protein with the reaction H(+) + L-aspartate = beta-alanine + CO2, the Schiff-base intermediate with substrate; via pyruvic acid, Proton donor must be optimized to ensure efficient catalysis.3. The protein design should consider the endogenous proteins and enzymes present in the beta- alanine from L-aspartate: step 1/1, Cofactor biosynthesis pathway to avoid interference with native metabolic processes.4. The substrate binding site should be located in a region of the protein that is accessible to the ligand.5. The protein must bind to pyruvate in order to perform its enzymatic function.6. The ideal protein would be one that plays a critical role in pantothenate biosynthetic process, alanine biosynthetic process through aspartate 1-decarboxylase activity within cytoplasm. The designed protein sequence is ",
    "response#1": "MXXRXXXNXQXAXDXRXQXXPXMXGXDXGXXLXPLXXLXWXKEXXQXXXXAXXXFXXXXXXQXXXXXXXHXXGX",
    "response#2": "MACERLSVELPGRGYTLRIGQGLLDRAGEVAAPLLRGKRVAVIHTPPVAALLGDALEAALAEAGFAVR",
    "response#3": "MGKNVVIIGAXTDIGLATAKRFAREGYNIAITSRTEEKVKNAADELRKEFGVESDFILADITKEEQIEKLFDSLGEIDVLVSNAGVATYGTVEETSLNQWKDVMDVNLTGVFLVTQIVAKKMVERKSGSIITIGSVAGKMASPFLGAYCTSKAGLIMLTKVLALEVADYGIRVNSISPGIIKTKFTEKIWEGKNKEIIENLQKRIPLKRFGLPEEVAEAVVFLASDKSSYMTGSDILVDGGWTIW"
  },
  {
    "reference": "MNLIMVISINIILSSILILVAFWLPQLNSYTEKANPYECGFDPMSSARLPFSMKFFLVAITFLLFDLEIALLLPIPWAIQTYNINTMLLAAFILVSILALGLAYEWKQKGLEWTE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The protein should have a fast turnover rate for a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +   NAD(+).3. The protein must exhibit the following characteristics: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor; Essential for the catalytic activity of complex I..4. The protein should be engineered to have a higher affinity for a specific molecule or receptor in respirasome, mitochondrial inner membrane that is critical for mitochondrial electron transport, NADH to ubiquinone. The designed protein sequence is ",
    "response#1": "YSLRIIYLVMMNYPRFQSLSPIDENYTKTRNPIKRLAWGSVIAGMMIFLNILPTKSQTMTMPTHMKTAAIMVTILGVLTAMELTKLTSTQLKITPNVNMHNSSNMLGYFPNIIHRLLPQTNLYLGQKMATHLTDQTWFEKIGPKGILALQIPTTKMINNSQQGLIKTYLTLFFLTTVLFTTGLTAYNYSLRIIYLVMMNYPRFQSLSPIDENYTKTRNPIKRLAWGSVIAGMMIFLNILPTKSQTMTMPTHMKTAAIMVTILGVLTAMELTKLTSTQLKITPNVNMHNSSNMLGYFPNIIHRLLPQTNLYLGQKMATHLTDQTWFEKIGPKGILALQIPTTKMINNSQQGLIKTYLTLFFLTTVLFTTGLTAY",
    "response#2": "SSHWLLAWMGLEISTLAIIPLMSQHHHPRAVEATTKYFITQAAAAALILFASTTNAWITGQWDINFDLHFFPASMLTMALALKMGLAPVHFWLPEVLQGLDLTTGLILSTWQKLAPFILMCQIMPVNSSLITFLGVTSTLVGGWGGLNQTQLRKILAYSSIAHLGWMILVMQFNQQLALLALIIYIPMTFSTFMIFKTNSSTTVNTLAASWAKTPALTAITPMILLSLGGLPPLSGFMPKWMILQELTKQDIPLTASIAALSALLNLFFYMRLAYSSSLTMFPTTNNTKMVWQFQPMNVSFMMTSLISISIMALPLTPLLS",
    "response#3": "LMSVSILCLFFLINPYQIMIYLMIMTLFLLAKGLINNAESFINFFFFDLMSLSMVILTVWISILMIMASKSNNNYKNKIFNFYLLLMMNLLFS"
  },
  {
    "reference": "MLKGKTVVLGVTGSIAAYKAANLASMLVKLHADVHVIMTKNATNFIHPITFETLTNNKCLVDTFDRNFQYSVEHVALAKKADVMMIAPATANVIGKLAGGIADDMLTTTALACTCPKLIAPAMNTRMFENSIVQDNLKKLTLNDYQVIQPAVGFLACRDEGAGKLPKEEVLLDYILKEIAFEKDMKGLKVLVTAGPTMEAMDPVRYITNHSTGKMGYAVARNCMLRGAEVTLVTGQTSIEKPRFVTVVPIESAREMFEEVTGRAAEQDIIIKAAAVADYRPQTVSDEKVKKGGDHLTLELEKTDDILKYLGEHKKEGQFLCGFSMETEHMLENSRAKLKKKNLDLIVANNLKQAGAGFGTDTNVITMITPEEEISLELMSKEEAAGRILDEILKLRR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be optimized for phosphopantothenoylcysteine decarboxylase activity, FMN binding, phosphopantothenate--cysteine ligase activity activity in order to improve pantothenate catabolic process, coenzyme A biosynthetic process.2. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A.3. The designed protein must have at least one Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domain.4. The protein should have a low Km value for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.5. The protein should have a high affinity for CTP.6. The protein design should take into account the physiological conditions of the Cofactor biosynthesis, coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway.7. The protein requires Mg(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "MKIVLGVAGGIAAYKACSLLRLLTEDGHDVTVVPTQAALRFVGAPTWAALSGRPVATEVWDAVHEVRHVRLGQEADLVVVAPATADLLARAAHGLADDLLTNVLLTASGPVVLAPAMHTEMWEHAATQANVAILSERGVHIVPPASGRLTGADTGPGRLPEPEELYAVLSAVVSETERPELAGVTVAVSAGGTREPIDPVRYLGNRSSGRQGVAVAVAAAERGARVLLAACNVEAEVLRPAEAAGVEVRRAGSAAELSEVMREVAERADAVIMAAAVADYRVDAVSERKLTKEDGEPPVLRLVENEDIVAGLAARRRPGQVVVAFAAETAVGEELLARASRKRRRKGVDLIVVNEVGWGKGFEVAENRLLGPDGEPVVDASGSKESVAHRVLDLVGRPGGPLEGLNVVVTAGGTREPIDAVRVIANRSSGKQGHAISEECVRRGANVTVVTTTELPTSPTTDRIEVETAAEMQIALDNVAPTADIVVMAAAVADFRPKDSVDGKIKKAGGVPEVILEPTPDLLEAIGRNRHPHQTIVGFAAETCDLVTNARSKLERKCLDLVVANDVSAEGVGFAHGTNAVTLLGADGSLTGRHIVVSAGGTREPLDPVRYLGNRSSGKQGYALARTALARGARVTLVAANTTLPDPPGALVVPVGTAEELADAVRSAVAEADAVVMAAAVADFRPAAYTEHKIKKTHDPGDPDAAPVIELVRNPDVLAGLVAARGDATTPVIVGFAAETGDESGDVLALGRAKLLRKGCDILVVNEVGADKTFGRDDNLVHI",
    "response#2": "MKIVLGVAGGIAAYKACSLLRLLTEDGHDVTVVPTQAALRFVGAPTWAALSGRPVATEVWDAVHEVRHVRLGQEADLVVVAPATADLLARAAHGLADDLLTNVLLTASGPVVLAPAMHTEMWEHAATQANVAILSERGVHIVPPASGRLTGADTGPGRLPEPEELYAVLTAVAEVLSGRGLEGLNVVVTAGGTREPIDAVRVIANRSSGKQGHAISEECVRRGANVTVVTTTELPTSPTTDRIEVETAAEMQIALDNVAPTADIVVMAAAVADFRPKDSVDGKIKKAGGVPEVILEPTPDLLEAIGRNRHPHQTIVGFAAETCDLVTNARSKLERKCLDLVVANDVSAEGVGFAHGTNAVTLLTPGEEPPEVPLGTKRDVAAAVVERVASALDGAAAA",
    "response#3": "MKIVLGVAGGIAAYKACSLLRLLTEDGHDVTVVPTQAALRFVGAPTWAALSGRPVATEVWDAVHEVRHVRLGQEADLVVVAPATADLLARAAHGLADDLLTNVLLTASGPVVLAPAMHTEMWEHAATQANVAILSERGVHIVPPASGRLTGADTGPGRLPEPEELYAVLQAALDPQALAGKKVVITAGGTVERIDPVRYISNDSSGKMGFALARAARAMGAAVTLIAARTDEAPPRDAGIDLVRVQSAQEMHDAVMDRWRDCDILVKAAAVADYRPRESSDSKIKKSGSTMTLELVKTTDILESLGRIKDKQFLIGFAAETGNAEAYAKDKLVRKNLDLIVANDVAVEGAGFGTDTNIVKLVDETGAEDWPRMAKEEVARRLAARIAEALA"
  },
  {
    "reference": "MMGRRGSSWCRWWVALLVLAVAADAVGCTSVSYDDRSLVIDGQRRIILSGSIHYPRSTPEMWPDLIKKAKEGGLDAIETYIFWNGHEPHRRQYNFEGNYDVVRFFKEIQNAGMYAILRIGPYICGEWNYGGLPAWLRDIPGMQFRLHNEPFENEMETFTTLIVNKMKDSKMFAEQGGPIILAQIENEYGNIMGKLNNNQSASEYIHWCADMANKQNVGVPWIMCQQDDDVPHNVVNTCNGFYCHDWFPNRTGIPKIWTENWTGWFKAWDKPDFHRSAEDIAFAVAMFFQKRGSLQNYYMYHGGTNFGRTSGGPYITTSYDYDAPLDEYGNLRQPKYGHLKELHSVLKSMEKTLVHGEYFDTNYGDNITVTKYTLDSSSACFINNRFDDKDVNVTLDGATHLLPAWSVSILPDCKTVAFNSAKIKTQTSVMVKKPNTAEQEQESLKWSWMPENLSPFMTDEKGNFRKNELLEQIVTSTDQSDYLWYRTSLNHKGEGSYKLYVNTTGHELYAFVNGKLIGKNHSADGDFVFQLESPVKLHDGKNYISLLSATVGLKNYGPSFEKMPTGIVGGPVKLIDSNGTAIDLSNSSWSYKAGLASEYRQIHLDKPGYKWNGNNGTIPINRPFTWYKATFEAPSGEDAVVVDLLGLNKGVAWVNGNNLGRYWPSYTAAEMAGCHRCDYRGAFQAEGDGTRCLTGCGEPSQRYYHVPRSFLAAGEPNTLLLFEEAGGDPSGVALRTVVPGAVCTSGEAGDAVTLSCGGGHAVSSVDVA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The Nucleophile, Proton donor active site of the designed protein must be optimized for substrate binding and specificity.2. A protein that contributes to carbohydrate metabolic process by functioning in vacuole, apoplast with beta-galactosidase activity, carbohydrate binding.3. The designed protein should contain one or more SUEL-type lectin domains that are essential for its function. The designed protein sequence is ",
    "response#1": "GRNYVPTWAFDHQKQFNGGSELQLILDKYTGTGFQSKGSYLFGHFSMHIKLPAGDTAGVVTAFYLSSTNNEHDEIDFEFLGNRTGQPAILQTNVFTGGKGNREQRIYLWFDPSKAYHTYSILWNMYQIVFFVDNIPIRTFKNAKDLGVRFPFNQPMKLYSSLWNADDWATRGGLEKTNWV",
    "response#2": "GRNYVPTWAFDHQKQFNGGSELQLILDKYTGTGFQSKGSYLFGHFSMHIKLPAGDTAGVVTAFYLSSTNNEHDEIDFEFLGNRTGQPAILQTNVFTGGKGNREQRIYLWFDPSKAYHTYSILWNMYQIVFFVDNIPIRTFKNAKDLGVRFPFNQPMKLYSSLWNADDWATRGGLEKTNWL",
    "response#3": "GRNYVPTWAFDHQKQFNGGSELQLILDKYTGTGFQSKGSYLFGHFSMHIKLPAGDTAGVVTAFYLSSTNNEHDEIDFEFLGNRTGQPAILQTNVFTGGKGNREQRIYLWFDPSKAYHTYSILWNMYQIVFFVDNIPIRTFKNAKDLGVRFPFNQPMKLYSSLWNADDWATRGGLEKTNWL"
  },
  {
    "reference": "MASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The presence of Protein kinase domains is critical for the interaction of the designed protein with its intended targets.2. The protein with the reaction ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-   threonyl-[protein], the Proton acceptor must be optimized to ensure efficient catalysis.3. A protein that can perform magnesium ion binding, protein serine/threonine kinase activity, peptide binding, protein serine kinase activity, ATP binding in cytosol, cytoplasm, nucleus, keratin filament, beta-catenin destruction complex to support Wnt signaling pathway, intermediate filament cytoskeleton organization, signal transduction, cell morphogenesis, protein phosphorylation.4. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.5. For general function, the protein need meet that May phosphorylate PER1 and PER2; May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration ; May play a role in segregating chromosomes during mitosis ; Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates ; Can phosphorylate a large number of proteins ; Acts as an inhibitor of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3.; Phosphorylates CTNNB1 at 'Ser-45'  The designed protein sequence is ",
    "response#1": "VMGDVLGEGSYGKVKEAMNSENLCRLAVKILTKRKLRRIPNGEQNVTREIHLLKQLKHTNVVGLVDVLYNEEKQKMYLVMEYCVGGLQEMIDDQPDKKMPLFQAHGYFQQLIDGLEYLHGCRVIHKDIKPGNLLLTLDQTLKISDFGVAEQLDLFSPDDTCTTGQGTPAFQPPEIANGHETFAGFKVDIWSSGVTLFNLATGLYPFEGDNIYRLLENIGRGQWVAPDWLYEMDAELANLIYGMLQADPSARLSLQQIRQNTWYISQYADFTNKYGLAYVLSNSYVGAYFNDSTKIVTLIESEIAYYMEHAKGTDGDGRRVLNVTQQHPHDTQKKKKFELKKVLGKGGYGKVFQVRKTAGRDANKYFAMKVLKKASIVTNQKDTAHTRAERNILEAVKHPFIVELVYAFQTDGKLYLILEYLSGGELFMHLEREGIFLEDTTCFYLSEIILALGHLHKLGIIYRDLKPENILLDAQGHVKLTDFGLCKEHIQEGIVTHTFCGTIEYMAPEILTRSGHGKAVDWWSLGALMFDMLTGVPPFTAENRKKTIETILKAKLNLPAYLTPEARDLVRRLMKRQEPQRLGSGPEDAAAVQIHPFFATTSWIAIYEKKVEAPFIPKYTGPGDASNFDDYEEEELRISINEKCAKEFSES",
    "response#2": "VMGDVLGEGSYGKVKEAMNSENLCRLAVKILTKRKLRRIPNGEQNVTREIHLLKQLKHTNVVGLVDVLYNEEKQKMYLVMEYCVGGLQEMIDDQPDKKMPLFQAHGYFQQLIDGLEYLHGCRVIHKDIKPGNLLLTLDQTLKISDFGVAEQLDLFSPDDTCTTGQGTPAFQPPEIANGHETFAGFKVDIWSSGVTLFNLATGLYPFEGDNIYRLLENIGRGQWVAPDWLYEMDAELANLIYGMLQADPSARLSLQQIRQNTWFEKCKEDVEDHYEMGEELGSGQFAIVRKCQQKGTGMEYAAKFIKKRRLPSSRRGVSREEIEREVSILREIRHPNIITLHDVFENKTDVVLILELVSGGELFDFLAEKESLTEDEATQFLKQILDGVHYLHSKRIAHFDLKPENIMLLDKHAASPRIKLIDFGIAHRIEAGSEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGETKQETLTNISAVNYDFDEEYFSSTSELAKDFIRRLLVKDPKRRMTIAQSLEHSWLQSSSEMNLETMIRV",
    "response#3": "VPPLRPIGRGASGIVCAAWNSETGEEVAIKKIGNAFGNIIDAKRTLREIKLLKHMDHDNVIAIIDIIRPPQPDNFNDVHIVYELMDTDLHHIIRSNQPLTDDHSRFFLYQLLRGLKYVHSANVLHRDLKPSNLLLNANCDLKIGDFGLARTKSETDFMTEYVVTRWYRAPELLLNCSEYTAAIDIWSVGCILGEIMTREPLFPGRDYVQQLRLITELIGSPDDSSLGFLRSDNARRYVRQLPQYPRQNFAARFPNMSVNAVDLLQKMLVFDPNRRITVDEALCHPYLSIHGPPPDPNLFELKKVLGKGGYGKVFQVRKTAGRDANKYFAMKVLKKASIVTNQKDTAHTRAERNILEAVKHPFIVELVYAFQTDGKLYLILEYLSGGELFMHLEREGIFLEDTTCFYLSEIILALGHLHKLGIIYRDLKPENILLDAQGHVKLTDFGLCKEHIQEGIVTHTFCGTIEYMAPEILTRSGHGKAVDWWSLGALMFDMLTGVPPFTAENRKKTIETILKAKLNLPAYLTPEARDLVRRLMKRQEPQRLGSGPEDAAAVQIHPFMATTSWIAIYEKKVEAPFIPKYTGPGDASNFDDYEEEELRISINEKCAKEFSELKRKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGF"
  },
  {
    "reference": "MTDYIFVTGGVVSALGKGITAASLGRLLKSRGVKVTIQKFDPYINVDPGTMSPYQHGEVFVTNDGAETDLDLGHYERFIDTDLSQNNNVTTGMVYGNVISKERKGEYLGSTVQVIPHITDEIKSRVRRAGQEGNFDVVITEIGGTVGDIEGLPFLEAIRQMKGELGPDRVCYIHCTLVPYLDTAGELKTKPTQHSVKELRSIGIHPDAIVCRADRELTEEVKDKISLFGDIEREAVIEAVDVEYIYQLPLDMQDEGLDEIVIDKLNLETEDLDLESWRRLVKKMKNRTKKARIGLIGKYVELPDAYLSIIEALDHAGIPHDCEVEIEWIESDDLDSADLEDELAHLDGIIIPGGFGERGIEGKVSAIKYARQQEIPFFGLCLGMQCAVIEFARSRAGLERAGTTEVDSEAEEPVIDLMPEQKDIEKMGGTMRLGRYPCRLEPETLARNLYGQEVVYERHRHRYELNNRYRSDLEEKGMVMSGLSPDERLVEIIELPEHPWFIGVQFHPEFTSRPNDPHPLFEGFIDAALNHQESSDFSG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be optimized for metal ion binding, CTP synthase activity activity in order to improve 'de novo' CTP biosynthetic process.2. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP, L-glutamine, CTP, UTP, Mg(2+) binding.3. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen4. The protein's Nucleophile must be carefully engineered to ensure that the amino acids involved in H2O + L-glutamine = L-glutamate + NH4(+) are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "LPDAYLSVVEALRHGGIANGVDVDIRWIASDDLAPNELGAAPTDDGQVGRLLAGVDGVLVPGGFGIRGVEGKIAAIRWAREHGVPFLGLCLGLQSAVIEFARNVVGLPHAHSAEFEPASPDPVIDLMHDQREVVDKGGTMRLGAYPAKLLEGTRTRAAYGDEPVIYERHRHRYEVNNRYRPRLEEAGLVFSGVSPDDRLVEFVELPDHPWFVATQAHPELKSRPTRPHPLFDGLLQAALAYQT",
    "response#2": "VTVALVGKYVDLPDAYLSVAEAIRAGGFAHGARANIRWVASDACETPEGAAEQLAGADAVVVPGGFGIRGIEGKIGAIAHCRESRIPLLGLCLGMQCAVIEAARRGGVPDASSSEFDEATPSPVIATMAEQLAAVSGEADLGGSMRLGAYPAVLAEGSVTADVYGATEVSERHRHRYEVNNAYRDAIESAGLVISGTSPDGALVEFVEYPREEHPFFVATQAHPEYKSRPTNPHPLFAGMIEAVLA",
    "response#3": "LPDAYLSVVEALRHGGIANGVDVDIRWIASDDLAPNELGAAPTDDGQVGRLLAGVDGVLVPGGFGIRGVEGKIAAIRWAREHGVPFLGLCLGLQSAVIEFARNVVGLPHAHSAEFEPASPDPVIDLMHDQREVVDKGGTMRLGAYPAKLLEGTRTRAAYGDEPVIYERHRHRYEVNNRYRPRLEEAGLVFSGVSPDDRLVEFVELPDHPWFVATQAHPELKSRPTRPHPLFDGLVAAALAR"
  },
  {
    "reference": "MLSYLRREWQSYGENATNVKEEEDTMSDKKKESKIEKPIKTENRYCYKSPQKIDKYESDLQGFSLGTQDEHINPTTLQIYDGMLTNGHTFISTNPPCSTIFELSIKAENGAMVDGKPLTDFSCFVISITKDENGVCDITYHCQSDLDDAQKRILLRGFSNKGCSGLNDVKINSILRVVDKALGSEFHTRTQASLYTWDRDCVENYSTKVRVDERKVGNSRVIIYQQEDGFWKILTETIWIDLRAVFKPYGIMGGAFKNWLVDSGFDKYEHQYSYEREGKMVSATTITYPKPTGKAGVNQPWRPATDYNGQYVCLQPGDTFSVWYFEDQWQIRNAIYAKNFQSDTMAEGTLENKGQLIFKMNYIPSLANIKNKQGKVQYRYINGGFAQVDASSYTGMALIFNFECIGKKFYTEDYKTKIDNSITPYICFIGKNYTPDGYFYEKGCCSGFAAGYDTETISHKMTISYTVMRPSDPDFVTGGDAYGQSITSSLEVSMRNLQDQINSIRAELNISQVTSAVLSAITSLGDLPNLFSNITQIYSKLKDALSKLKIKKSKPKPIKATMIVDRNTVDVPNVSIMNRMPEELEVGIIYNSMRNTKKHDISKFALSTELELPYIQTTSTLTPKFTKYLEQKGLLTVDDIAVQFDPLNTTFSTLRRKNAEIMKYKIDPEVAHEVLSQMSNSATRSLFSLNVRKQISTHNEFSTPTYEQLINRILNDKEILDVLGKLNPHSVGNMFQEFVDRMQDMLSYY",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein contains novel FHA domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "ITCRVSLLDGTDVSVDLPKKAKGQELFDQIMYHLDLIESDYFGLRFMDSAQVAHWLDGTKSIKKQVKIGSPYCLHLRVKFYSSEPNNLREELTRYLFVLQLKQDILSGKLECPFDTAVQLAAYNLQAELGDYDFAEHSPELVSEFRFVPIQTEEMELAIFEKWKEYRGQTPAQAETNYLNKAKWLEMYGVDMHVVKARDGNDYSLGLTPTGVLVFEGDTKIGLFFWPKITRMDFKKNKITLVVVEDDDQGKEQEHTFVFRLDHPKACKHLWKCAVEHHAFFRLRR",
    "response#2": "RMQESLFEVVTSEASYLRSLRLLTDTFVLSQALRDTLTPRDHHTLFSNVQRVQSVSERFLGTLLSRVRSSPHITDLCDVVHAHAVGPFFVYVDYVRNQQYQEETYSRLMDTNVRFSAELRRLQSLPKCERLPLPSFLLLPFQRITRLRMLLQNILSQTEEGSSRQENAQKALGAVSKIIERCSAV",
    "response#3": "MQESLFEVVTSEASYLRSLRLLTDTFVLSQALRDTLTPRDHHTLFSNVQRVQSVSERFLGTLLSRVRSSPHITDLCDVVHAHAVGPFFVYVDYVRNQQYQEETYSRLMDTNVRFSAELRRLQSLPKCERLPLPSFLLLPFQRITRLRMLLQNILSQTEEGSSRQENAQKALGAVSKIIERV"
  },
  {
    "reference": "MLLSKLSILLAKWLSVAVYAGTLVHDEQFIPDHILRVSVAQVPSACENREDVVVNGTSPGPAIHLLPGARTWIRVYNDMNDRNLSMHWHGLSQRFAPFSDGTPSATQWPIPPGHFFDYEILTEPEDAGTYFYHSHVGMQALSCTGPLIVEDCGSSPYHYDDERILLFQDHFQKSDLEMIQGLTSTQFTWTGETRGILLNGRGVSPNQAAVQGRPGEASGFFGSHRFSNFRAGDGTSNSWDGIRGDDQIEPPTDCTLPVIDVEPGKTYRLRFIGATGLSLLTMGFEDHNDLTIVQVDGSEYNAPVTVDHIQLGGGQRFDVLLRTKTAEELRCNGDKTTYFLQFETRDRPDPYRGYGVLRYNLGTPVPAAPTTPALTLPAEVNNWLEYTFQPLHPSSSLSPTAEEVTRRVILEAEQKIDPATGRLVWKLAHMTWTDMSRDKPVLVDIYERGEAAMPDYAAALTNYGWDPATKLFPAKKDEVLEIVIQNTGSHYSGASGIVETHPFHAHGQHFYDVGSGPGKYDPEANNAKLASLGYRPIKRDTTMVYRYGEGKVAPGEPAGWRAWRMKMNNPGVWMVHCHILAHMIMGMETIWVVGDAEDIVTIPLSVSQNYFTYGGSVYGNDTHAPEVYHYFDDTNKCCAAGAGDSEDSGH",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of Plastocyanin-like domains is critical for the interaction of the designed protein with its intended targets.2. The protein should be designed to have high stability and activity towards 2 2',3,4-trihydroxy-trans-chalcone + 2 H(+) + O2 = 2 3',4'-   dihydroxyaurone + 2 H2O.3. Ensure the presence of a signal peptide within the protein.4. For general function, the protein need meet that Can be used for the bioconversion of 2',3,4-trihy-droxychalcone to 3',4'- dihydroxy-aurone, a bioactive aurone recently shown to possess inhibitory activity against several isoforms of the histone deacetylase complex (HDAC).5. The designed protein should have a binding site that is specific to the target Cu cation ligand.6. The protein must exhibit oxidoreductase activity, copper ion binding. The designed protein sequence is ",
    "response#1": "MVLSDWSRFTSEEYWKAIEDSKLLLFCVDSILLNGHGEVYCPPQEFLVNQTQWGPQHFTFPDQNVTDKGCFPAVEEGIQGPWVNQSLPEKIPAHIQSGCVPSAGSNYTVEVDPADGWVSMNFIAAASNKQVDFSIDEHPMWIYEVDGNYVQPHKFVAAAITAGERFSVMVKLDKQPGRYTIRLPDSGATQVISGFAFPVTLDKVVQVKVARPKKSRSAEEKEAEEEILLIVGIEVEIDKYAKFDVYLNDSDDPSGGKDKAEYAGSFAHLPHKHKGMKKIRTTLSLGLNEPLEDLGAEDDDTILVTLAPKVGGGVVSVDNVKVG",
    "response#2": "DEYNVFVGDWYNKGHKTLKKILDGGRTIGRPDGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSHTVQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRTPLKLVEYFGATNKAFKYDLMADEAPADPSKLTIATNVKNATYRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPNSWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSGTYSFYCSPHQGAGTFPVTLDKVVQVKVARPKKSRSAEEKEAEEEILLIVGIEVEIDKYAKFDVYLNDSDDPSGGKDKAEYAGSFAHLPHKHKGMKKIRTTLSLGLNEPLEDLGAEDDDTILVTLAPKVGGGVVSVDNVKVFPVTLDKVVQVKVARPKKSRSAEEKEAEEEILLIVGIEVEIDKYAKFDVYLNDSDDPSGGKDKAEYAGSFAHLPHKHKGMKKIRTTLSLGLNEPLEDLGAEDDDTILVTLAPKVGGGVVSVDNVKVK",
    "response#3": "MVLSDWSRFTSEEYWKAIEDSKLLLFCVDSILLNGHGEVYCPPQEFLVNQTQWGPQHFTFPDQNVTDKGCFPAVEEGIQGPWVNQSLPEKIPAHIQSGCVPSAGSNYTVEVDPADGWVSMNFIAAASNKQVDFSIDEHPMWIYEVDGNYVQPHKFVAAAITAGERFSVMVKLDKQPGRYTIRLPDSGATQVISGFATPLKLVEYFGATNKAFKYDLMADEAPADPSKLTIATNVKNATYRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPNSWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSGTYSFYCSPHQGAGCRNFYQQALVHCAYCNGGYDQVNFPDQEIQVHNSWLFFPFHRWYLYFYERILGKLIGDPSFGLPFWNWDNPGGMVLPDFLNDSTSSLYDSNRNQSHLPPVVVDLGYNGADTDVTDQQRITDNLALMYKQMVTNAGTAELFLGKAYRAGDAPSPGAGSIETSPHIPIHRWVGDPRNTNNEDMGNFYSAGRDIAFYCHHSNVDRMWTIWQFLGPILKGEIGDTIVVHFKNNGSRVYSMHPHGVFYSKDSEGALYEDNTKGKFKKDDKVPPGGTHTYSWHLTQSHAPADQEDKCITWIYHSHVVPSKDINTGLLGIMLIG"
  },
  {
    "reference": "SFAGLNDADVAAALAACAAADSFNHKAFFAKXXXXXXSPDDLKKXXXXLDQDKSGFLEEDELKLFLQNFSASARALTDAETKXXXXAGDADGDGMLGLDEFAVLVKQ",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Ca(2+) binding.2. The designed protein should contain one or more EF-hand domains that are essential for its function.3. The protein must exhibit the following characteristics: In muscle, parvalbumin is thought to be involved in relaxation after contraction; It binds two calcium ions..4. The designed protein must be optimized for calcium ion binding. The designed protein sequence is ",
    "response#1": "MIQDVKKAFFVIDQDKSGFIEEDELKLFLQNFSAGARALTAGETKTFLAAGDSDGDGMIGVDEFQALR",
    "response#2": "EDQVKKIFGILDQDKSGFIEEDELQLFLQNFSSTARALTAAETKAFMAAGDTDGDGKIGVDEFQALA",
    "response#3": "GKELAACLDVFDDARSGVIPAEQLRQAMVSHGDRLTEEEADEMVRKADPAGEGRVEYKEFVKVG"
  },
  {
    "reference": "MASATVRNVPLLDDDTIPFGEEDEMRDPSRAGQKYTHPYVTFFHLFFRGAAILIYMFCGWFSDSFITSFVFVVLFLSADFWTVKNISGRLLVGLRWWNYVDDDGVSHWVFESKNSESYQSRVNKNEQRIFWLGLILCPVFWGLFFLFALFGLKFKWLLLVMIAIALNAANLYGYVKCNYGANKDLNSAATDFVKTQFFKNAVDIMTRPSGAPPPTNVRPTGVV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a specific function in protein secretion and should be localized to Golgi membrane in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "MTGAPVIFVPGNAGSYKQVRSLASVALRKAMSNDAGIHLDYYTIDYDEELSALYGGYLHRQQSYLKLCIRTILSIYEGRTEQPSIVLIGHSMGGKLAQSVLVDPAIGQHINTIISISTPLDQPVLNLDTQLEEFYDQTDAVLSKLRTATVPTMTTNVCDSLHQRPPSVQRMASQDSSARLDNVLLISTGGGNRDLLVRPGLTSSRFNDLHAMTSAIPKVSLSCDHLSAVWCLQFMQAINRFLFSS",
    "response#2": "MTGAPVIFVPGNAGSYKQVRSLASVALRKAMSNDAGIHLDYYTIDYDEELSALYGGYLHRQQSYLKLCIRTILSIYEGRTEQPSIVLIGHSMGGKLAQSVLVDPAIGQHINTIISISTPLDQPVLNLDTQLEEFYDQTDAVLSKLRTATVPTMTTNVCDSLHQRPPSVQRMASQDSSARLDNVLLISTGGGNRDLLVRPGLTSSRFNDLHAMTSAIPKVSLSCDHLSAVWCLQFMQAINRFLFSG",
    "response#3": "MTGAPVIFVPGNAGSYKQVRSLASVALRKAMSNDAGIHLDYYTIDYDEELSALYGGYLHRQQSYLKLCIRTILSIYEGRTEQPSIVLIGHSMGGKLAQSVLVDPAIGQHINTIISISTPLDQPVLNLDTQLEEFYDQTDAVLSKLRTATVPTMTTNVCDSLHQRPPSVQRMASQDSSARLDNVLLISTGGGNRDLLVRPGLTSSRFNDLHAMTSAIPKVSLSCDHLSAVWCLQFMQAINRFLFSL"
  },
  {
    "reference": "MPSEQKQLFCDEKQTTLKKDYDVKNEIVDRSAPKPKISGSIHYALKNVKIDLPKINIPNEVLLKHEVDKYRKLFQSKQQTARKSISIKTVSCVEECTLLHKSERAEEEGVKMSAKILNFSCLKCRDNTRYSPNDLQKHFQMWHHGELPSYPCEMCNFSANDFQVFKQHRRTHRSTLVKCDICNNESVYTLLNLTKHFTSTHCVNGNFQCEKCKFSTQDVGTFVQHIHRHNEIHYKCGKCHHVCFTKGELQKHLHIHSGTFPFTCQYCSYGATRREHLVRHVITLHKEHLYAKEKLEKDKYEKRMAKTSAGLKLILKRYKIGASRKTFWKRKKINSGSDRSIEKNTQVLKKMNKTQTKSEDQSHVVQEHLSEEKDERLHCENNDKAPESESEKPTPLSTGQGNRAEEGPNASSGFMKTAVLGPTLKNVMMKNNKLAVSPNYNATFMGFKMMDGKQHIVLKLVPIKQNVCSPGSQSGAAKDGTANLQPQTLDTNGFLTGVTTELNDTVYMKAATPFSCSSSILSGKASSEKEMTLISQRNNMLQTMDYEKSVSSLSATSELVTASVNLTTKFETRDNVDFWGNHLTQSHPEVLGTTIKSPDKVNCVAKPNAYNSGDMHNYCINYGNCELPVESSNQGSLPFHNYSKVNNSNKRRRFSGTAVYENPQRESSSSKTVVQQPISESFLSLVRQESSKPDSLLASISLLNDKDGTLKAKSEIEEQYVLEKGQNIDGQNLYSNENQNLECATEKSKWEDFSNVDSPMMPRITSVF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should emphasize Basic and acidic residues compositional bias.2. The ideal protein would be one that plays a critical role in regulation of transcription by RNA polymerase II through metal ion binding, DNA-binding transcription factor activity, RNA polymerase II-specific within nucleus. The designed protein sequence is ",
    "response#1": "MVFEDVAIYFSQEEWGILNDAQRHLHSNVMLENFALLSSVGCWHGAKDEEVPSKQCVSVRVLQVTIPKPALSTLKAQPCKMCSSILKDILHLAN",
    "response#2": "LTLEDVAVEFSWEEWQLLDTAQKNLYRDVMVENYNHLVSLGYQTSKPDVLSKLAHGQEPWC",
    "response#3": "LTLEDVAVEFSWEEWQLLDTAQKNLYRDVMVENYNHLVSLGYQTSKPDVLSKLAHGQEPWTTDAKIQNKNCG"
  },
  {
    "reference": "MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should aim to mimic or enhance the function of natural proteins involved in cellular response to osmotic stress, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "LIATGSGHLIKGILWCGDVTMDRLIWGNGFMKRRLSKAEKESEVHPDTLKRIRRVKRMTKMTESVANSILSGVLKVSGFFTSSVANTKVGKKFFSLLPGEVILASLDGFNKVCDAVEVAGRNVMSTSSTVTTELVDHKYGGKAAEATNEGLDAAGYALGTAWVAFKIRKAINPKSVLG",
    "response#2": "GGSLGRDAATGRGVMYATEALLTEYSESISGSTFVIQGLGNVGSWAAKLIHQKGGKIVAVGDVTGAIRNKSGIDIPALLKHRSEGGSLEDFYGAEVMDAAELLVHECDVLVPCALGGVLNRENAAEVKARFIIEGANHPTDTEADEILAKKGVIVLPDIYANSGGVVVSYFEWVQNIQGFMWDEEKVNRELQKYMKNAFQNIKDMCKSQNCNLRMGAFTLGVNRVAV",
    "response#3": "IVDTVAEVQFLIYLPGYRKQDVHILSYGDYLVVKGQRFSYFNEQDFRQKEGKYGSFEKKIPLSDHLHGKMNAIFKDGILYITIQKDEGQAC"
  },
  {
    "reference": "MKKRNITEFQVLSEIIRKQPHIKQKEIAENLGITVQAVSEHIRNLVKEGYVKSRGRGEYVVTEKGLRKLKNWISEFKDYLDEINTAVYRYKDIWPAIADEDVKDGETVYLFMKNGLLYASKQPKGEAKAKALYGGKKGEDIAICEIKGIIDVPKGKVIVFRIPPEVVGGSRAVDFNLIKENIDNLDDYVIATMGTVAYVVACKLGLKPDIRFAVPEAIVNACNRGCNVIALITGKMAEKVIKKLDNAKISYTVLDATKENK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must be optimized for DNA binding. The designed protein sequence is ",
    "response#1": "IPNKVIKYALENQWFAQLKERYTTVRSEVPYGKDNKSRVDFLLTQNEGKSPIYVEVKNTTLCQGEIALFPDTVTSRGQKHLRELMALLPDTKPIMLYFINRGDCYSFAPGDEFDPTYGTLLREAVKKGLEVLPCRFEVTPEL",
    "response#2": "IPNKVIKYALENQWFAQLKERYTTVRSEVPYGKDNKSRVDFLLTQNEGKSPIYVEVKNTTLCQGEIALFPDTVTSRGQKHLRELMALLPDTKPIMLYFINRGDCYSFAPGDEFDPTYGTLLREAVKKGLEVLPCRFEVTPEQ",
    "response#3": "IPNKVIKYALENQWFAQLKERYTTVRSEVPYGKDNKSRVDFLLTQNEGKSPIYVEVKNTTLCQGEIALFPDTVTSRGQKHLRELMALLPDTKPIMLYFINRGDCYSFAPGDEFDPTYGTLLREAVKKGLEVLPCRFEVTPEY"
  },
  {
    "reference": "MVPLIALQSTKRAELGRAVIGALKHLTHSPANDDGDDTAPDANPGQLHADEHSLPETGSTHSSTVPVVTIDPVPYEIAATEADPQQPTPSAKTNAAALYTQTHVKNQEGNAMMEYSSSSDSASSNNDESNNTPRLAVPGEDTIHVSNSTADLLSLAIQRNASMHSLPASEQDSLASITANCIEECQPETRRGELLYTPATPTSAMSFTDYRHESDDDDEDEYEMPSVADGEDATSKNRHLGQRLHNMLHATHQHVHLPHRHGGKIIDAVAAKDGALTPESSGDSVTDAEESSRAWHRVREYHGDLSSYLQRIHVEHNINERTAWAATESVHVKHLLDEMLGLAQDNAAYFRNKATVKPTFGSRPRAESAERFEMLFFRAHSTVLAIRHDTFSLCWLAPLFDFDEHVKSSGYRSLVRVVMACLSKLAKEMRAFTLSRDSLLFRADASFVVMEDYIKTLELLSAMLNIALRMASLGHSNSVFPGDASLGPLPASTLAAADSLLAEVEGIQCARFYGRKFGFQYSKPMRTTLKVILIAMASYSYGFNNNKSDLAQKVFALASSPLYVFGSEDRARSLSKQLLRGDLAFSKAFWNLTENTMAKHVSKVVSANVSVNFVMKIPVVALTCPADDPSRYPAGGNVHLSVETLTNDGLFDVVHARLISHEWYAGQMYECAHVERGGESESRLFTKAKPMSNALIVHFHGGGFISQSSKSHEIYLREWTRELDCPIVSVDYTLAPDAPYPQALFECLFTYVWALKNAARLGTTAETV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should take into account the physiological conditions of the Lipid metabolism pathway.2. The protein should have hormone-sensitive lipase activity, acylglycerol lipase activity and be active in lipid droplet, caveola.3. The protein should have a low Km value for cholesteryl (9Z-octadecenoate) + H2O = (9Z)-octadecenoate +   cholesterol + H(+).4. The designed protein should contain one or more Alpha/beta hydrolase fold-3 domains that are essential for its function. The designed protein sequence is ",
    "response#1": "VFTALYDVCEENAAFFSGATKGQAGDAAQRLVDAMTTIQEHARSLEPVISGFAAIYHHFDFDPHIPANGYRSLVKVVRCCLLHIIHKGRYITSNRRSIFFRVSHSATEMEVYCSALCQLRALLYLAQRLLHDNSQGNLFFQEESGISQSFLREYASMHKGCFYGRCVGFQSAVHLSVYLFVFLSGVAASSLLTSGKYAIDPDLRGQEYERITQNLDVHFWKTFWNITETESLVSMTATQVKVNRALSVPPMPFDLPLVADPTHTVTVSPPAAHIGPGPVQMRLISYELREGQR",
    "response#2": "TQSLVTLAEDNMAFFSSQGPGETARRLSSVFAGVREQALGLEPALGRLLGVAHLFDLDAETPANGYRSLVHTARCCMAHLLHKSRYVASNRRSIFFRTSHNLAELEAYLAALTQLRALTYYAQRLLATNRPGGLFFEGDEGLTSDFLREYVTLHKGCFYGRCLGFQSLALVHFHGGGFVAQTSKSAF",
    "response#3": "VFTALYDVCEENAAFFSGGTKAQAGDTSQRLVDSMTTIQEHARSLEPVITGFAAIYHHFDFDPHVPANGYRSLVKVVRCCLLHIIHKGRYITSNRRSIFFRASHNAAEMEAYCSALCQLRALLYLAQRLLHDNSHGNLFFQEESGLSQSFLREYASMHKGCFYGRCVGFQFTPAIRPCLQSITIGLVAFGENYKKHQSGIGVAASSFFTSGKYAIDPELRGQEYERITQNLDVHFWKAFWNITETQILSSLVSMTATQVKVNRALSVPPEPFDLPLAADPTRMVTVSPPSSHVGLGPVQMRLISHELREGQE"
  },
  {
    "reference": "MVVWNVSTTFQTPERPVAFSDFMADVDSGKVEQVTITGHAIAGIYRTDNENFRTYAPSQYEGLAKKLIERGILVTATDPTQSSLTSLLYSWAPILLLIGGLIFFVRQMPRGGNTLLSFGKSKATLSSNLQKKVTFKDVAGVDEASEELQEIIAFLKEPEKFQKLGGRIPKGVLLMGPPGTGKTLLARAVAAEANVPFFAISGSDFVEMFVGVGASRVRDLFAQGKQHAPCIVFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFESNEGVILVAATNRPDVLDPALLRPGRFDRRIVVNRPDVRGREGILAVHTRKSPLSDDVDTAVLARGTAGFSGADLANLVNEAALHAARDNRQAVGMIDFEFAKDKVLMGTERRSMIISDAEKRITATHEAGHALLAVLLPHADPVHKVTIIPRGMALGLTQQLPVDEKHHDSKDDLDDQIAILMGGRIAEEIATVGIMTGAGNDLERATELARRMVCEWGMSAMGPLTFGKKVAPIFLGRDFAQHQDYSEYTALRIDQEIKRIVSANYERAHRILVEYKAVLVNIADELLTREVLDGEQVRRMVRGLSLNEPQPAASTGPAAAVPDEEDTRRRQKKRPSLVPPLTDPLPQA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins.2. The designed protein must possess ATP hydrolysis activity to support cell division under certain conditions. The designed protein sequence is ",
    "response#1": "MPKGILLVGPPGTGKTLLARAVAGEAKVPFFSISGSEFVEMFVGVGAARVRDLFAQAQKSAPCIIFIDELDALGRARGLGSIAGGPDEKEQTLNQLLVEMDGFDPRQGVVLLAATNRPEILDGALLRAGRFDRQILVDRPD",
    "response#2": "MPKGILLVGPPGTGKTLLARAVAGEAKVPFFSISGSEFVEMFVGVGAARVRDLFAQAQKSAPCIIFIDELDALGRARGLGSIAGGPDEKEQTLNQLLVEMDGFDPRQGVVLLAATNRPEILDGALLRAGRFDRQILVDRPTLE",
    "response#3": "MPKGILLVGPPGTGKTLLARAVAGEAKVPFFSISGSEFVEMFVGVGAARVRDLFAQAQKSAPCIIFIDELDALGRARGLGSIAGGPDEKEQTLNQLLVEMDGFDPRQGVVLLAATNRPEILDGALLRAGRFDRQILVDRPGFAELLGIGDVQAAL"
  },
  {
    "reference": "MRTHTQERPYKCPEDDCGKAFTASHHLKTHRRTHTGEKPYPCQEDSCQKSFSTSHSLKSHKKTHQRQLQNKGRKKRPLKTQQTKCSDQEQKDPQQEEQEEEEFIKEDQPEMTLLNPGSHCSETTSTDSGVVLQTLTPQDHLSNVFILQGNEPLILPETSQAYQLSYAAEEEIPSPWIDAGVLVSKPIIPMAPLTDACVALPTEMPSFVNLKPTFGNAVSGNMGDPQPETMDVDPTVETSLPTPTLELNQPNIEELLKQDSFENEEDMETESLLNDILMTIDNNSALLQATLQQASQVPSDASGLVELDIRDNKPTLKQITADAGICNCTNCKCDQTKSCHGGDCGAAAAQQTTRTGAPTTTTTTTTMNSSGGKRICGSVVPTKKVSKRETEMNQNIEDVALLLQNLASMSSGGSSGGGGGCCGGGAVKPAPSGGGCCGEPKAPKPVNNGCGCARPSAPSSAGCCGSGVASTPKPTGGCCSGNQKPAPTATAASSPSCCCSGKGEVQEPPVISSASSAPAAAVKGNACTCKSPAEGVANGCCVVICIKTLQALRKVLTRRNLNLMLCPQQN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The presence of C2H2-type domains is critical for the interaction of the designed protein with its intended targets.2. A protein that can perform transcription cis-regulatory region binding, DNA-binding transcription factor activity, RNA polymerase II cis-regulatory region sequence-specific DNA binding, DNA-binding transcription factor activity, RNA polymerase II-specific in nucleus to support stress response to metal ion, positive regulation of transcription by RNA polymerase II. The designed protein sequence is ",
    "response#1": "MLENYGNVASLGFPLLKPAVISQLEGGSELGGSSPLAAGTGLL",
    "response#2": "QHTKGIGSKLMAKMGYIFGKGLGKDGEGRVEPIEVVVLPQGKSLDKP",
    "response#3": "YKCEECGKAFKRSSTLTIHKMIHTGEKR"
  },
  {
    "reference": "MTSPRGGEETSPVRVAIAGYKGKVGSVLAAAFQIEPGIDYVGGIGRGDDLATFLHEKRPRALVDFTRPTEAMHNALAAVAAGAAPVVGTTGLSTADIDKLETACHAKHVGGIVAPNFAIGAVLMMHLAETAAPHFDAVEIIEMHHAAKLDAPSGTALSTARRLAARRKDRPFTHRKAEKETLAGTRGGEEGGVGVHSVRLPGFVADQEVIFGLPGQTLTIAHRTTSREAYVPGVLLAIRRVTAEPRFYRGLDQLLGLRSD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should exhibit Basic and acidic residues compositional bias.2. The protein must exhibit the following characteristics: Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate..3. The protein contains novel Dihydrodipicolinate reductase C-terminal, Dihydrodipicolinate reductase N-terminal domains that confer a unique function or activity.4. The designed protein is the optimization of the Proton donor, Proton donor/acceptor to achieve high catalytic efficiency for (S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-   4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH.5. We require a protein that can specifically bind to the metabolites produced in the (S)-tetrahydrodipicolinate from L-aspartate: step 4/4, L-lysine biosynthesis via DAP pathway, Amino-acid biosynthesis pathway.6. The (S)-2,3,4,5-tetrahydrodipicolinate, NAD(+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MKVAVAGYRGKVGSVLAAALAAEPEMDYVGGVTRGDDLASFLHEKRPQALVDFTRPSEAVHNALAAIAAGAAPVVGTTGLSSADIDKIETACHEKKVGGIVAPNMAIGAVLMMKFSKMAARYFADVEIMEMHHDQKLDAPSGTAVKTAELIAEVRESKKQGHPDEKETIPGARGADFDGMRIHSTRLPGLIAHQQVMFGAEGESLTIRHDSYNRTSFMSGVKFAVEEVLKVNVLIYGLENILDL",
    "response#2": "MDILLVGFGAMNKRVAAIAEESGHKIAGVVTPEAKIDASYPIFTNFDNPLPEVDVVIDFSHPTLTTQLLKSTISVPLIIATTGDKDTLVELMQKKAKTQPVFFSANMSVGAVLMMKFAKMAAAYFPDVEIIEKHHDQKLDAPSGTAKKTAELIQEVRKRKKQGHPDEEETIQGVRGGDFDGMRIHSMRLPGLVAHQEVVFGGAGQTLTIQHDSMNRASFMDGIKECVYQVMDLDELIYGLENVLGLE",
    "response#3": "MDILLVGFGAMNKRVAAIAEESGHKIAGVVTPEAKIDASYPIFTNFDNPLPEVDVVIDFSHPTLTTQLLKSTISVPLIIATTGDKDTLVELMQKKAKTQPVFFSANFAIGAVIMIIISKLSARYFENCEIIEMHHENKKDAPSGTSISTAESIGKIINYNESKLNKDEQENIEGSRGAFTNGIHLHSIRLPGLLAHQCVIFGSKGQTLTIKHDSFDRSSFYPGLIMAIKNIDNLSNFTYGLESLGLQ"
  },
  {
    "reference": "LICFICFSPTAH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein can be expressed in extracellular region and perform toxin activity without triggering undesirable cellular responses.2. The protein must exhibit the following characteristics: Probably blocks muscarinic acetylcholine receptors (CHRM), since it inhibits the binding of the selective muscarinic ligand [3H]QNB and inhibits the carbachol-induced [3H]inositol phosphate accumulation in rat hippocampus.. The designed protein sequence is ",
    "response#1": "GNILVMVSIKVNRHLQTVNNYFLFSLACADLIIGIFSMNLYTLYTVIGYWPLGPVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPVKRTTKMAGMMIAAAWVLSFILWAPAILFWQFIVGGRTVPDKDCYIQFFSNPAVTFGTAIAAFYLPVIIMTVLYWQISRASKSRIKKGKKEAAQNQDPVSPSLVQGKIVKPNNNNIPTSSDGLEHNKVQNGKTTGESVMENCVQGEEKDSSNDSTSVSVVPSNTKEDEAAKDASQISASQDHLKVENSKLTCIRIVTKSQKGDCCAPTNTTVEIVGTNGDEKQNSVARKIVKMTKQPAKKKPPPSREKKVTRTILAILLAFIITWTPYNVMVLINSFCASCIPGTVWTIGYWLCYINSTINPACYGNAMVVMAYRIERNISKQVSNRYIVSLAISDLIIGIEGFPFFTVYVLNGDRWPLGWVACQTWLFLDYTLCLVSILTVLLITADRYLSVCHTAKYLKWQSPTKTQLLIVMSWLLPAIIFGIMIYGWQAMTGQSTSMSGAECSAPFLSNPYVNMGMYVAYYWTTLVAMLILYKGIHQAAKNLEKKAKAKERRHIALILSQRLGTQVGVSLMLQSKAEKEKAEEAQKDSGYTSNQAGDANNLRRFGFSEPETSQFRVDPNSNNNLNVEGSLNTENDQNLGVIEEERSGFLSRRESNESYYPGPHPTAANSRRCSEMEKVSLLSESDGVPSTRPAKSYGRLSLRSRYSASESITTTHENDEKEVEKADSLQKLFADDELGSVLNFKEEKLKNTDSNNDSDTTSVILQRSRKYKKNKRPRSSRRSEHSTPRQIAKVKQAEGTAAQLIEESVPDDDQTETIEVKRTDRWVVSMKKRIARALIRRRSTTRPERGSSSNSDDSSSEVEGEEKPEVRNNGLKIPQLTVNNENRGETSSQPGRDRLAPPNKTDTFLSASGVSRKISTISTVITREKVISSIFAPIAVFNRGRKQTKAEKRAHKAFRTITFIVGFFAILWSPYYIMATVYGFCKGECIPSFLYTLSYYMCYLNSSGNPFA",
    "response#2": "GNVLVMISFKVNSQLKTVNNYYLLSLACADLIIGIFSMNLYTTYILMGRWVLGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTPKRAGIMIGLAWLVSFILWAPAILCWQYLVGKRTVPPDECQIQFLSEPTITFGTAIAAFYIPVSVMTILYCRIYRETEKRTKDLADLQGSDSVAEVKKRKPAHRTLLRSFFSCPRPSLAQRVRNQASWSSSRRSTSTTGKPTQATDLSADWEKAEQVTNCSSCPSSEDEAKATTDPVFQVVCKNEAKESPGKEFNTQETKETFVSPRTENNDYDTPKYFLSPGAAHRLKSQKCVAYKFRLVVKADGTQETNNGCRKVKIMPCSFPVSKDPSTKGLDPHLSHQMTKRKRMVLVKERKAAQTLSAILLAFIITWTPYNIMVLVSTFCDKCVPVTLWHLGYWLCYVNSTINPICYALRNEKIKQAFKKMMK",
    "response#3": "GNILVIVSFKVNKQLKTVNNYFLLSLACADLIIGVISMNLFTTYIIMGHWALGNLACDLWLSIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWIISFVLWAPAILFWQYFVGKRTVPLDECFIQFLSEPIITFGTAIAAFYLPVTIMSILYWRIYKETEKRTKELAGLQASGSEAETARFVHQTGSSRSLSSYELQRQSTKRSSRRKYRRCHFWLTMKSWEPNTDQGDQEHSSSDSWNNNDAAASLENSASSDEEDITAETRAIYSIVLKLPGHSAILNSTKLPSSEDLNESADELQKSDTDSQEKKPKKLQPPKSIQDGGSFQKSFSKLPIQPGSAETATASDGISSVTKTSAALPLSFKEATLAKKFALKTRSQITKRKRMSLIKEKKAAQTLSAILFAFIITWTPYNIMVLVNTFCDCVPKTVWNLGYWLCYINSTVNPVCYALCNATFKKTFKHLL"
  },
  {
    "reference": "MKDNKLEKPKPIRKISNKKAPKPPRFSFMWVYAILIFVFLIVTLVLNDSTGKPISFQYFAENMLKKGDVERIVAYKNGDFVTADIYIKKDSLKKKTQLYGDVAKDQNAFSSWQSTSSQYQITDASMESLKASLANAEKGLPESDQIMPEYKQNESVFSNPLVQFIIMGVLLVAVWLFIMRRMSGGSGGGPGGQIFNIGKSKATLFDKEAQVSVTFNDVAGLEEAKQEVMEIVDFLKNPKKYTNLGGKIPKGALLVGSPGTGKTLLAKAVAGEAQVPFFSLSGSDFVEMFVGVGASRVRDLFRQAKDKAPCIIFIDEIDAIGRARGKNNIVGGNDERENTLNQLLVEMDGFGTDSGIIILAATNRPDVLDSALLRPGRFDRQVSIDKPDLNGREQIFKVHLKPVKLAAGVDAKKLSAQTPGFAGAEIANVCNEAALIAARKNKEAVDMQDFQDAIDRVIGGLEKKNKIISPEEKRIVAYHEAGHAIAGWFLEHADPLVKVSIVPRGVAALGYAQYLPKEQFLYTTEQLEDGMCMTLGGRVAEDIVFGKISTGAQNDLERITKLAYAMVTIYGMNEKVGNVSFNDTQGEYQFNKPYSEKTSELIDTEVRNQINTVYARTKQLLTDKREGLEKLANKLIEKEILFQSDLEEILGKRPFDHRTTYDEFVNGTPESNQEPAAQSLVHDGVGDRSGTFERNPEEKEASSSNE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must have a active site that is structurally stable and resistant to denaturation.2. A protein that can perform ATP binding, metalloendopeptidase activity, ATP-dependent peptidase activity in plasma membrane to support protein catabolic process, cell division.3. The designed protein should have a high specificity for ATP, Zn(2+) binding, with minimal off-target effects.4. The protein must exhibit the following characteristics: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins.5. Target a Polar residues, Basic and acidic residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "MVMTEDEKKLTAYHEAGHAIVGLNVPDHDPIHKATIIPRGRALGLVLSLPERDHLSVSYTKYRSKIAMAMGGKVAEELIFGADKVTSGAASDIQQVTRIARAMVTQFGMSEQLGNIDYANEQQSYLGTGYGQTSASPETQRVIDAEVRRLVDEGYEKAKSILTEKAGDLERLAQGLLEYETLTGDEIAAGVREMIAQ",
    "response#2": "MVMSEEEKTLTAYHEAGHAIVMLNVKGHEPLHKVTIIPRGRALGVTMFLPERDRLSQSRVQLDALVTSLFGGRVAEELIFGEENITTGASDDIRRATDICRRMVTEFGYSEKLGPLRYAVNQEEVFLGHSVTQQQNMSDATARLIDEEIRRLVEEGETRCRGILEEHLDALHALAKGLLEYETLSRDEIDRLL",
    "response#3": "MVMTEDEKKLTAYHEAGHAIVGLNVPDHDPIHKATIIPRGRALGLVLSLPERDHLSVSYTKYRSKIAMAMGGKVAEELIFGADKVTSGAASDIQQVTRIARAMVTQFGMSEQLGNIDYANEQQSYLGTGYGQTSASPETQRVIDAEVRRLVDEGYEKAKSILTEKAGDLERLAQGLLEYETLTGDEIAEWSRLTA"
  },
  {
    "reference": "MNLVGSYQAHHHHHHMLHEPFLFSPVSRCHPERSYFQSWVLNPAEMSPEFPGQPHSSSSYSSPEFGAPGQGHGPSSRLEALNGRLGRRKGAGPKKERRRTESINSAFAELRECIPNVPADTKLSKIKTLRLATSYIAYLMEVLAKDSQAGADPEGFKAELKKSDSRESKRKRETQTEVYSHSLGPGEKRLKGRTGWPQQVWALELNQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The ideal protein would be one that plays a critical role in angiogenesis, mesoderm formation, negative regulation of DNA-binding transcription factor activity, cell differentiation through enzyme binding, transcription coregulator binding, bHLH transcription factor binding, protein homodimerization activity, DNA-binding transcription activator activity, RNA polymerase II-specific within nucleolus, cytoplasm.2. The presence of BHLH domains is critical for the interaction of the designed protein with its intended targets.3. Prioritize Polar residues, Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "MSGLGDSSTDPANPDSRKRKGSPCDTAQSNEKRRREQENKYLEELAELLSANIGDIDTLSVKPDKCKILKKTVDQCA",
    "response#2": "MSGLGDSSTDPANPDSRKRKGSPCDTAQSNEKRRREQENKYLEELAELLSANIGDIDTLSVKPDKCKILKKTVDQC",
    "response#3": "MSGLGDSSTDPANPDSRKRKGSPCDTAQSNEKRRREQENKYLEELAELLSANIGDIDTLSVKPDKCKILKKTVDQC"
  },
  {
    "reference": "MEIALVPLENGGAMTIRGGGEARAGCGQAVGGELQCPPTAGLGDGPKERAPKGRGAQRGVDPGGRPLPPLPQELPQPRRLPPEDEEGEGDPALGMAEDQMLGAGSLHHQRVLINISGLRFETQLGTLAQFPNTLLGDPAKRLRYFDPLRNEYFFDRNRPSFDGILYYYQSGGRLRRPVNVSLDVFADEIRFYQLGDEAMERFREDEGFIKEEEKPLPRNEFQRQVWLIFEYPESSGSARGIAIVSVLVILISIITFCLETLPEFRDERELLRHPPAPHQPPGPARGANGSGALAPPSGPTVAPLLPRTLADPFFIVETTCVIWFTFELLVRFFACPSKAEFSRNIMNIIDVVAIFPYFITLGTELAEQQPGGGGGGGQNGQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGKTLQASMRELGLLIFFLFIGVILFSSAVYFAEADNQETHFSSIPDAFWWAVVTMTTKHRSGGLRQSWDTRESEGPPDSSRQDCQSQHHPQPFTTEEKERGPVVAGSTPKRDSTGPSHQGKHNTNFCWKTRGKRAFKPLQRRSRLQLLSSTPQV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be able to modulate protein homooligomerization in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "MVFLQGVAGIGKSLMLTKLMLAWSEGMVFQNKFSYIFYFCCQDVKKMKRASLAELISKEWPNASAPIEEILSQPEKLLFVIDNLEVMECDMSEWESELCDDCMEKQPVNILMSSLLRRKMLPESSFLVSATPETFEKIEDRIECTNVKMMAGFNESSIKVYFYSLFQR",
    "response#2": "MVFLQGVAGIGKSLMLTKLMLAWSEGMVFQNKFSYIFYFCCQDVKKMKRASLAELISKEWPNASAPIEEILSQPEKLLFVIDNLEVMECDMSEWESELCDDCMEKQPVNILMSSLLRRKMLPESSFLVSATPETFEKIEDRIECTNVKMMAGFNESSIKVYFYSLFQR",
    "response#3": "MVFLQGVAGIGKSLMLTKLMLAWSEGMVFQNKFSYIFYFCCQDVKKMKRASLAELISKEWPNASAPIEEILSQPEKLLFVIDNLEVMECDMSEWESELCDDCMEKQPVNILMSSLLRRKMLPESSFLVSATPETFEKIEDRIECTNVKMMAGFNESSIKVYFYSLFQR"
  },
  {
    "reference": "MNEESRCPVTGAANRQATGRSTSNRDWWPNQLNLKILHQHSRMSNPMGEEFNYAEEFKKLDLEALKKDLYALMTDSQDWWPADYGHYGGLFIRMAWHSAGTYRMGDGRGGAGSGTQRFAPLNSWPDNANLDKARRLLWPIKQKYGSKISWADLMIRAGNCALESMGFKTFGFGGGREDVWEPEEDIYWGSEDTWLGDKRYSGDRDLENPLAAVQMGLIYVNPEGPSGNPEPVASGRDVRETFARMAMNDEETVALVAGGHTFGKCHGAGDAALVGPEPEGAGIEEQGLGWKSGFGSGKGDDTITSGIEGAWTPKPTQWDMGYFDMLFGYEWELVKSPAGAKQWVAKDVAEKDMVPGAHDPSKRRAPIMTTADLSLRFDPIYEPISRRFHKNPEEFADAFARAWFKLTHRDMGPRSRYLGAEVPAEELIWQDPVPAVDHELIDAQDIADLKGKILASGLSISELVSTAWALASTFRGSDKRGGANGARIRLAPQKDWQINQPAQLSTVLQTLEGVQKEFNGAQPGGKRVSLADVIVLGGCAGVEQAARNAGHDVTVPFMPGRTDASQEQTDVESFAVLEPVADGFRNYLKTKYAVSAEELLVDRAQLLTLTAPEMTVLVGGMRVLNANFGQSRHGVFTKRPEALSNDFFVNLLDMSTTWKATSEDEDVFEGRDRATGELKWTGTRVDLIFGSNSQLRALAEVYGCENSQKKFVHDFIEVWNKVMNLDRFDLA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be optimized for catalase activity, metal ion binding activity in order to improve response to oxidative stress.2. The protein design should incorporate a binding pocket that specifically recognizes heme b to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "FARAWFKLTHRDMGPKARYLGPDVPAEDLVWQDPIPAGRSDYDVAAVKARIAASGLSASDLVATAWDSARTYRGSDHRGGANGARIRLAPQKDWEGNEPQRLARVLSVLEPIAAETGASLADVIVLGGNLGVEQAAKAAGFDIEVPFAPGRGDSTAEQTDAASFDVLEPLADGFRNWQKQDYVVSPEEMLLDRAQLMGLTAPEMTTLIGGLRVIGTNHGGTKHGVFTQRKGALTNDFFVTLTDMANNWVPVGNNLYEIRDRKTGATRYTATRVDLVFGSNSILRAYAEVYAQARAWFKLTHRDLGPKANYFGVDVPKEDLIWQDPIPTGNTDYHIIDVKDKIAASGLSVSEMVTTAWDSARTYRGSDKRGGANGARIRLAPQKDWEGNEPKRLAKVISILEPIASEFEISVADTIVLAGNVGIEQAAKDAGFDITVPFSAGRGDATDEMTDAESFEPLEPLADGYRNWSKKDYVVSPEELMLDRTQLMGLTAPEMTVLVGGMRMLDTNYNNTKHGILTDNVGHLTNDFFVNLTDMKYTWKPVADNLYEICDRKTGKVIWTATRMDLVFGSNSILRSYAEVYAQY",
    "response#2": "AFARAWFKLTHRDMGPKSRYLGPEVPKEDLIWQDPLPAATHQPSAEDIASLKTAIAGAGLSVSELVSVAWASASTFRGGDKRGGANGARLALAPQKDWPVNAIASRVLPTLQAIQRASGKASLADIIVLAGVVGVEQAAAAAGVSVNVPFTPGRVDALPEQTDVESFDLLQPLADGFRNYRRIEGGVSTETLLIDKAQQLTLTAPEMTVLVGGLRVLGANYDGSKHGVFTDRVGVLSNDFFVNLLDMATVWKAADDNAELFTGSDRKTGEAKYSATRVDLVFGSNSVLRALAEVYS",
    "response#3": "ISWADLIVLAGTVAYEAAGLKTYGFAFGREDIWHPEKDTYWGEEKEWLAPSDSRYGDVTKAETLANPLAAVQMGLIYVNPEGVNGKSDPQATAYQMRETFARMGMNDEETVALTAGGHTVGKCHGNGNVTDLSPDPEAAGPEFQGLGWMNTKGRGIGRNTMVSGLEGAWTTHPTQWDNGFFEMLFKHEWTLTHSPAGASQWEPITIAEEDKPADVEDPSIRHMPMMTDADMALKVDPIYREISLRFMNDFEAFSDAFARAWFKLTHRDMGPKARYLD"
  },
  {
    "reference": "MPKHGKKYLAALAKVDRTRLYSPTEALALVKETSYTKFDGTVEAHLRLGIDPRHADQNIRTTVALPHGTGKTVRVLVFAQGEAVQAALDAGADYAGSDDLIARIDRENFFDFDVAIATPDMMGKVGRIGRKLGPRGLMPNPKSGTIVPAADLARTIREVKGGRVEIRNDKTGILHVAIGKVSFTPQQLSENFVALMDAVKAAKPSGAKGTYIRSVTLTSTMGPGVPVDPVAAQNLKA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have structural constituent of ribosome, tRNA binding within cytosolic large ribosomal subunit to regulate maturation of LSU-rRNA, regulation of translation.2. The protein must exhibit the following characteristics: Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA.. The designed protein sequence is ",
    "response#1": "MR",
    "response#2": "EPRKGNVQKREVLPDPIYNSKLVTRLINHMMMDGKRGTSTKIIYGAFDIIKNETGNDPVETFEEAMKNIMPVLEVKARRVGGSNYQVPIEVRPDRRTTLGLRWLVQYSRLRGEHTMVERLAREIMDAANNTGAAVKKREDTHRMADANL",
    "response#3": "PRKGNVQKREVLPDPIYNSKLVTRLINHMMMDGKRGTSTKIIYGAFDIIKNETGNDPVETFEEAMKNIMPVLEVKARRVGGSNYQVPIEVRPDRRTTLGLRWLVQYSRLRGEHTMVERLAREIMDAANNTGAAVKKREDTHRMADANR"
  },
  {
    "reference": "MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLEKTVPMFNDALAEINKIAARKGKVLFVGTKRAASESVKESAISCDQYYVNNRWLGGMLTNWKTVRQSIKRLKELEIQSADGTFEKLTKKEALMRTREMEKLEKSLGGIKNMGGLPDAMFVIDADHEHIAIKEANNLGIPVFSIVDTNSNPDGVDFVIPGNDDAIRSIQLYTGAVAQTVTEARNQDIVVQAEQDGFVAEA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that contributes to translation by functioning in small ribosomal subunit with structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "MSEAKYKIDRRMGQNIWGRPKSPVNRREYGPGQHGQRRKGKLSDFGVQLRAKQKLKGYYANISERQFHAIYVEATRLKGDSGENLIGLLG",
    "response#2": "MIL",
    "response#3": "GRRLQTIVYKKGLARTIYQARQLITHGHIAISGRKVTSPGYVVLRGEEDLIDYYPTSPFKQNPQ"
  },
  {
    "reference": "MGLSDGEWQLVLTVWGKVEADLAGHGQEVLIRLFKNHPETLEKFDKFKNLKSEDEMKGSDDLKKHGNTVLSALGGILKKKGQHEAELKPLAQSHATKHKIPVKYLEFISEAIIQVLQSKHPGDFGADAQGAMSKALELFRNDMAAKYKELGFHG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.2. The protein must be able to oxygen carrier activity, heme binding.3. The heme b binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "AALSKVLAQNAEAFGAEALARMFTVYAATKSYFKDYKDFTAAAPSIKAHGAKVVTALAKACDHLDDLKTHLHKLATFHGSELKVDPANFQYLSYCLEVALAVHLTEFSPETHCALDKFLTNVCHELSSRYR",
    "response#2": "AALSKVLAQNAEAFGAEALARMFTVYAATKSYFKDYKDFTAAAPSIKAHGAKVVTALAKACDHLDDLKTHLHKLATFHGSELKVDPANFQYLSYCLEVALAVHLTEFSPETHCALDKFLTNVCHELSSRYN",
    "response#3": "DVIDQQHRRIIEMLNHLHVAQASMQRAAVGEVIDEVVDYTMSHFAFEEELMEEAGYPFCAAHKRVHEVFIKRVSEYRMRFQAGEDISDELRTMLSRWLFNHIRGDDQAYAS"
  },
  {
    "reference": "MTSPLSVIILAAGKGTRMQSAKPKVLQTLAGKSLLGHVLDTCHQLTVNETIIVHGFGGDQVKSEITNQYTQASITWVAQTEQLGTGHAVKVTLSDLPKSGQSLILYGDVPLVSCQTLTALKTANTNGMSMLTLTVDNPFGLGRIKRDQAGNIEAIVEQKDADAEEQSIREINSGIYCVDNALLHKYLPELSNDNAQQEYYLTDIVKMAVADGITIAAIEPEHTFEIEGVNNRQQLASLERTWQRKLVADLQEAGVQFADPTRVDIRGTLSAGQDVFIDVNVVFEGDCTLGDNVYIEAGCIIKNAKIGNACYIKPYCVIDRADIGAGVDIGPFAHLRPDTVLSDNSKVGNFVEIKKSTIGKGSKVNHLSYVGDATVGTGVNIGAGVITCNYDGANKSQTIIEDNAFIGSNASLVAPVTIGDTATVAAGSVITKDVDRSALAFGRARQVQKNDFQRPIKK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.2. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "LPKVLHPICGRPMLAYVLDAAREATGQRPLVVYSPATAQIRDVFADEADFALQDQPRGTGDAVRVALSSLDDGATNLLVLNGDKPLLVAAPLRSLMQRQMDATAAMTLTTFVVSSNSPWGRVDFGPDGQAERIVERKDDPGRAELSAFHANAGIYAFDLAWLRGAVPRVEPSSASGELYLTQLVEIACKDGCPVATIDPAAAAGRIRSGEFVG",
    "response#2": "VVVLAAGQGTRMRSRRAKVLHEICGMPMLSHIQAVAGEIEPERLIVVVGRGAEAVSQILGESAELVVQAEQKGTGHAVMMAEESLADFDGDVLVLNGDTPLLRADTLLRMRALKAETGAALTMLTAVEDPKGYGRILRTQDNEVMRIVEEKDSTPEERAITEIYSGIMAVPTKFAKQWLANLESNNAQGEYYLTDIVALAVNDGIPVKAQHIDDIWQTLGVNSKVQLAELERIYQANQAEALMESGVTLLDPTTAYVDSRVKIGQDTIIHPNVTISGLSEIGDDCVIESGVYILDSRLGDGVRVLQGSRLDRAEVHDGTSVGPMAHLRPEATIGRNAKIGNFVEVKKSVIGDGSKASHLTYLGDSSIGKNVNIGCGTITCNYDGKKKHRTVIHDRCFVGSDVQFVAPVEVGPGSVIGAGSTITRDVPEKSLAVSRTKQVNKPGWQRPVKKD",
    "response#3": "LPKVLHPICGRPMLAYVLDAAREATGQRPLVVYSPATAQIRDVFADEADFALQDQPRGTGDAVRVALSSLDDGATNLLVLNGDKPLLVAAPLRSLMQRQMDATAAMTLTTFVVSSNSPWGRVDFGPDGQAERIVERKDDPGRAELSAFHANAGIYAFDLAWLRGAVPRVEPSSASGELYLTQLVEIACKDGCPVATIDEA"
  },
  {
    "reference": "MEKLKFEIQSVPLTLEKAPVICGVVGSGNLEVLVSQHDQADVCQVEVTTSAVGFQHVWQSVLNEFAQRHAVAGLKLQLNDMGATPAVVNLRLSQAISMFKGE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Subunit of malonate decarboxylase, it is an acyl carrier protein to which acetyl and malonyl thioester residues are bound via a 2'-(5''-phosphoribosyl)-3'-dephospho-CoA prosthetic group and turn over during the catalytic mechanism..2. The protein can be targeted to cytoplasm and perform acyl carrier activity. The designed protein sequence is ",
    "response#1": "LTAISG",
    "response#2": "MVILE",
    "response#3": "MXXXGXVX"
  },
  {
    "reference": "MVAVLARQGRELAKRLLLIQFSAVMVAAAVFAVAVNGDWGLSALVGGGIFVIANAVFAGCAFLFAGARALKMVAISFYTGEALKILITIVLFSVAYMYMQLELVPLKLTYLLALGINICAPVLFINNKK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "VLFWLCILSFFSVLNEMVLNVSLPDIANHFNTTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNHFFILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGGIIAHYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYNWTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGGLIFSIVAGFISMVPYMMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVFILGSLSISISFLTIAFFVEFSMWLTTFMFIFVMGGLSFTKTVISKIVSSSLSEEEVASGMSLLNFTSFLSEGTGIAIVGGLLSLQLINRKLVLEFINYSSGVYSNILVAMAILIILCCLLTIIVFKRSNR",
    "response#2": "VLFWLCILSFFSVLNEMVLNVSLPDIANHFNTTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNHFFILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGGIIAHYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYNWTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGGLIFSIVAGFISMVPYMMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVFILGSLSISISFLTIAFFVEFSMWLTTFMFIFVMGGLSFTKTVISKIVSSSLSEEEVASGMSLLNFTSFLSEGTGIAIVGGLLSLQLINRKLVLEFINYSSGVYSNILVAMAILIILCCLLTIIVFKRSKKL",
    "response#3": "VLFWLCILSFFSVLNEMVLNVSLPDIANHFNTTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNHFFILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGGIIAHYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYNWTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGGLIFSIVAGFISMVPYMMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVFILGSLSISISFLTIAFFVEFSMWLTTFMFIFVMGGLSFTKTVISKIVSSSLSEEEVASGMSLLNFTSFLSEGTGIAIVGGLLSLQLINRKLVLEFINYSSGVYSNILVAMAILIILCCLLTIIVFKRSK"
  },
  {
    "reference": "MKFLDQAKVYIRSGNGGAGAISFRREKFIEFGGPDGGDGGRGGDVWALVVDGLNTLIDYRYQQHFRAKTGGHGKGRNMTGQKGDDIILKVPVGTQIFEEDNTTLICDLTEVGQRYRLAKGGNGGFGNLHFTTSTNRAPRRANPGLSGEERTLWLRLKLIADAGIIGLPNAGKSTFLASVTAAKPKVADYPFTTLYPHLGVARIDAREFVLADIPGLIEGAHEGVGIGDRFLGHVERCRVLFHLISAQEEDVVKAYQIVRNELKAYGNNLNDKTEIIALSQIDTLTIEERKAKQEFLQKVTGKSVMMFSAVSREGLENLLRAGAHIIEMARKKDVVREEQD",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must be able to magnesium ion binding, GTPase activity, GTP binding.2. For general function, the protein need meet that Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control.3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.4. The protein should have a high affinity for Mg(2+). The designed protein sequence is ",
    "response#1": "LTWNIDGLDESNVAERARAVCSCLALYTPDVVFLQEVIPPYCEYLKKRAVSYKIITGNEDEYFTAMMLKKSRVKLISQEIVPYPSTLMMRNLLVANVNISGNSICLMTSHLESTKDHSKERLKQLDTVLKKMMDAPPSATVIFGGDTNLRDQEVAKIGGLPNTILDVWEFLGKPEHCRYTWDTKLNNNLRACYTSRLRFDRILYRASMEGSQVIPQFLNLVGTEKLDCGRFPSDR",
    "response#2": "VPLFAKHYCDKGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKAILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDR",
    "response#3": "LTWNIDGLDESNVAERARAVCSCLALYTPDVVFLQEVIPPYCEYLKKRAVSYKIITGNEDEYFTAMMLKKSRVKLISQEIVPYPSTLMMRNLLVANVNISGNSICLMTSHLESTKDHSKERLKQLDTVLKKMMDAPPSATVIFGGDTNLRDQEVAKIGGLPNTILDVWEFLGKPEHCRYTWDTKLNNNLRACYTSRLRFDRILYRASMEGSQVIPQFLNLVGTEKLDCGRFPSDGWL"
  },
  {
    "reference": "MGNSRSSALSREVLQELRASTRYTEEELSRWYEGFQRQCPDGRIRCDEFERIYGNFFPNSEPQGYARHVFRSFDTNDDGTLDFREYIIALHLTSSGKTHLKLEWAFSLFDVDRNGEVSKSEVLEIITAIFKMIPEEERLQLPEDENSPQKRADKLWAYFNKGENDKIAEGEFIDGVMKNDAIMRLIQYEPKK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should be able to enhance the efficiency of visual perception. The designed protein sequence is ",
    "response#1": "KEQFFHEEECGDLVTRAVLVDMEPKVVSKTLSMAGRSGKWKYDISSQFSQKQGCGNNWANGYCVHGPKHQDIVMDLVRKQVEKCDRLGGFFTIMSMAGGTGSGMGTFLTRCLRDAYPDSLLLNQVIWPYGTGEVIVQNYNSILTLSHLYRSSDALLVHENDIIHKVCSQLMNIKQISFRDVNKVIAHQLGSIFQPVYTSC",
    "response#2": "KEQFFHEEECGDLVTRAVLVDMEPKVVSKTLSMAGRSGKWKYDISSQFSQKQGCGNNWANGYCVHGPKHQDIVMDLVRKQVEKCDRLGGFFTIMSMAGGTGSGMGTFLTRCLRDAYPDSLLLNQVIWPYGTGEVIVQNYNSILTLSHLYRSSDALLVHENDIIHKVCSQLMNIKQISFRDVNKVIAHQLGSIFQPVYTSC",
    "response#3": "VVTVQLGQCGNQVGYEFFDILCSDLHSKPGHSTKRENDYYQTVCKEQFFHEEECGDLVTRAVLVDMEPKVVSKTLSMAGRSGKWKYDISSQFSQKQGCGNNWANGYCVHGPKHQDIVMDLVRKQVEKCDRLGGFFTIMSMAGGTGSGMGTFLTRCLRDAYPDSLLLNQVIWPYGTGEVIVQNYNSILTLSHLYRSSDALLVHENDIIHKVR"
  },
  {
    "reference": "MMVESESIRSTPTSQNGVGSLPNQSDSCVGREGSGSGETNGELNPAELLHFQQQQALQMARQLLLQQATCLNSPSTDNKQPSVQVPVSVAMMSPQMITPQQMQQILSPAQLQAVLQQQQALMLQQLQEYYKKQQEQLHLQLLSQQQAGKQQPKELALGNKQLAFQQQLLQMQQLQQQHLINLQRQNLVGLQSGQGPVPMQSLPQVSPSDLHQLLKEMSSSQEESSKQDTVDLMTSITTSFPTTKVSPPTMHPSLSNGQNTRRESTSHYESSHLLYGHGECRWPGCEALCEDMGQFIKHLNTEHALDDRSTAQCRVQMQVVQQLEIQLAKESERLQAMMTHLHMRPSEPKPFSQPLNLVSSASNNKMSHDTFPDGLPQPPTSATAPITPLRQGTSVISSSSLPSVGPVRRRIVDKFCTPISSELAQNHEFYKNAEVRPPFTYASLIRQAILETPDRQLTLNEIYNWFTRMFAYFRRNTATWKNAVRHNLSLHKCFVRVENVKGAVWTVDELEYQKRRPPKMTGSPTLVKNMISGLGYSALNASYQAALAESSFPLLNSPPLHNSSGSVLHGGHDDVTSTGEPGNSNGSSPRLSPQYSQSIHVKEEPAEDDVRPASISAPTNQTTVLPEDRDLESESPMEDLP",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be involved in negative regulation of DNA-templated transcription by localizing to nucleus and exhibiting sequence-specific DNA binding, DNA-binding transcription factor activity, metal ion binding, DNA binding.2. The protein must exhibit the following characteristics: Transcriptional repressor.. The designed protein sequence is ",
    "response#1": "LEMKPLWEEFNQLGTEMIVTKAGRRMFPPFQVKILGMDSLADYALLMDFIPLDDKRYRYAFHSSAWLVAGKADPATPGRVHFHPDSPAKGAQWMRQIVSFDKLKLTNNLLDDNGHIILNSMHRYQPRFHVVFVDPRKDSERYAQENFKSFIFTETQFTAVTAYQNHRITQLKIASNPFAKGFRR",
    "response#2": "MYPGTLRSPGGSSTAGVGTSGGSGSPLPASNFTAAPVYPHYVGYPHMSNMDPHGPSLGAWSSPYSPPREDWSTYPGPPSTMGTVPMNDMTSPVFGSPDYSTLGPTSGASNGGSLPDAASESLVSLDSGTSGATSPSRSRHSPYAWMRKTVQVTGKR",
    "response#3": "LEMKPLWEEFNQLGTEMIVTKAGRRMFPPFQVKILGMDSLADYALLMDFIPLDDKRYRYAFHSSAWLVAGKADPATPGRVHFHPDSPAKGAQWMRQIVSFDKLKLTNNLLDDNGHIILNSMHRYQPRFHVVFVDPRKDSERYAQENFKSFIFTETQFTAVTAYQNHRITQLKIASNPFAKGFRK"
  },
  {
    "reference": "MPKLILCRHGQSEWNAKNLFTGWADVKLSKQGIEEAQSAGKKIYDNQIEIDIAFTSLLTRALETTQYILAGSDQQWIPVYKSWRLNERHYGGLQGLNKDDARKKWGEDQVHQWRRSYDVRPPRESEEQREAYLKNRRYQHIDHRMMPYCESLKDTLERVVPFWTDHISQHLLDDKTVLVSAHGNSIRALIKYLEGLSEEDIVGYEIKTGAPLVYELTDDLVVKDKYYL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should interact with enzymes and substrates within the glycolysis, Carbohydrate degradation, pyruvate from D- glyceraldehyde 3-phosphate: step 3/5 pathway to enhance or regulate its activity.2. A protein with 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity is needed to enhance glycolytic process in a specific context.3. The Proton donor/acceptor, Tele-phosphohistidine intermediate of the protein must be engineered to enable effective catalysis of (2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate. The designed protein sequence is ",
    "response#1": "MKYLLLIPDGMADWEVEELDGRTPLEVAETENMDFLAKEGACGIAKTVPDGFEPGSDVANLTILGVDVRKHYTGRGPIEALARGVKGKLVFRCNLVKVEDGVMVDYSGGRISDEEARKVIEELNRAKPYDFVKFYAGKSYRNLLVINKDFRDDVKTFPPHDITGKEIDKHLPSGGELAELLKELMGWSAEILPEITDKANMIWPWGGGRMPSFPNFGQRYGLRGAMITEVDLLEGIARGMGMEVVQVEGITGYIDTNYRGLVRETARALEEHDFVVLHTEGIDEVGHEGDAELKVRAIELYDSKIVGKLLNKVDLDETRILLLPDHPTPVKVKTHVAEPVPFTLYGRGRDEVKVYTEKSCRRGKFGLVDGLRLMDLLIKS",
    "response#2": "MKYLLLIPDGMADWEVEELDGRTPLEVAETENMDFLAKEGACGIAKTVPDGFEPGSDVANLTILGVDVRKHYTGRGPIEALARGVKGKLVFRCNLVKVEDGVMVDYSGGRISDEEARKVIEELNRAKPYDFVKFYAGKSYRNLLVINKDFRDDVKTFPPHDITGKEIDKHLPSGGELAELLKELMGWSAEILPEITDKANMIWPWGGGRMPSFPNFGQRYGLRGAMITEVDLLEGIARGMGMEVVQVEGITGYIDTNYRGLVRETARALEEHDFVVLHTEGIDEVGHEGDAELKVRAIELYDSKIVGKLLNKVDLDETRILLLPDHPTPVKVKTHVAEPVPFTLYGRGRDEVKVYTEKSCRRGKFGLVDGLRLMDLLIKG",
    "response#3": "INKAIEEGTLPENDALKNAIQHVKDNNSALHLMGLLSDGGVHSHINHLFGLLEMARVKNIEKVYVHPILDGRDTPPRSAEKYLNQLEEKLAELGIGEIATVSGRYYTMDRDNRWDRTKKSYDALVLGEGLEASDAISALKQSYAADKSDEFVIPTVIKKNGQPVGTIKDNDAVIFYNFRADRARQITKALALEEFDEFERPAEHPENLYYVCMAEYDG"
  },
  {
    "reference": "MALSVVILAAGKGTRMRSHLPKVLHPIAGCSMLEHVINTAKALNPHKIVVVAGHGIEQVKDQLRETPVTIVEQAEQLGTGHAVDQALPVIEDGDQVLVLYGDVPLIKASTLNDLIESQPSSGIGLLTVKLNNPMGYGRIIRNERNEVTQIVEQKDANEEQLKVNEVNTGILCADAKSLKGWLSNIDNNNAQGEYYLTDCIAMAAQGAGVKACHPTSEIEVEGVNSRVQLAQLERRYQEAKAQELLEQGVTLLDPARLDIRGELKCASDVLIDVNVIIEGTVTIETGASIGANVILKNCSIGADTVIKPNSMIEDSIIGKQCSVGPFARIRPGTTLEDEAFIGNFVETKKTVLGKGSKASHLAYLGDAKIGQGVNVGAGVITCNYDGVNKFVTTIEDGAFIGSDSQLVAPVTVGRNATVGAGTTVTKDIPAEDLCISRIPQKLISGWQRPVKETIKSNKKEEKQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a high specificity for acetyl-CoA, Mg(2+) binding, with minimal off-target effects.2. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc); The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain. The designed protein sequence is ",
    "response#1": "VIVLAAGEGTRMKSAKAKVLHSVAGRSLLGHVLHAVDHLKAKQVRIVVGSGRELVEEHISHIAPKASTVFQERRGGTGHAAQLALAGLSAKGTVLVLAGDTPMLTGESLAAFIDAHNEGKFAASVLTAEHPDPTGYGRIIRDDAGELARVVEDKDASDAQRAIREINSGVYAFDIALLREALSRLSTDNVQGEFYLTDTVALARADGLRVAAHAAPDPWEVEGVNDRAQLAVLGAELNRRIVRGWQREGVTLLDPMTTWIDVTVTLDRDAVVDQNTQLRGATTVGAAAAVGPDVTLIDTTVGAGAVVLRSHAVGAEIGPQASVGPYAYLRPAARLADKAKVGTFVEVKNSEIGPGAKVPHLSYVGDATIGARANIGAATIFVNYDGVAKHHTTVGEAAFVGCDTNLIAPVEIGPGAYVAAGSAITQDVPPGALGVTRASQRNIDGWVARRKAGTPAAEAAERVQQEASG",
    "response#2": "MTVVVLAAGEGTRMKSKTPKVLHEISGRSLVGHVVAAARELDPQHLVVVVGHASEQVTAHLNAGDEPVRTAFQAEQNGTGHAVRMGLEELGGTVDGTVIVVCGDTPLLSGETLGALAATHAADANAVTVLTLTVEDPTGYGRIVRDAEGRVTRIVEQKDATDDERLVREINSGIYVFDSRVLRGALGRLSTDNVQGEQYLTDVLGIARADGDPVGAFVARDAVETLGCNDRVQLSGLRRELNRRTVERWMREGVTVVDPASTFIDAEVRLAQDVTILPFTRLMGKTYVGEDAVIGPQTSLENCRVGCGSEVTYTVAKDSNIGDRCHIGPFAYLRPGTSLESDVKVGDFVEIKNSKIAAGAKVPHLSYIGDAQVGKAANIGAGTITCNYDGSSKHPTIIGDHSFIGSNTNLVAPVEVGDYAVTGAGSTITKDVPAKALAVERSQQRNIEGWVERRRAGTRSAAAAERALAAQAEAGPDGTDGGGR",
    "response#3": "GIILAAGKGTRMKSEIPKVMHPLCGRPMLAYVLDLAKTLKLDRTIVITGHGKEQLQGLLAEYKAKSVYQDRLVGTADAVRRTEGTLKGFSGNLLILYGDQPLLKAATLKKLIQRHISSFASATIALLTVILDDPTGYGRVIRKGEAVQAIVEHKDASEAQRQIKEVYTGIMAAPTAKLKQWLGRLNNNNAQGEYYLTDVIAMAVADQVPVVSAHPADAIETLGVNSKVQLAELERIHQRRQADALMEAGVTVVDPASTFIDAEVRLAQDVTILPFTRLMGKTYVGEDAVIGPQTSLENCRVGCGSEVTYTVAKDSNIGDRCHIGPFAYLRPGTSLESDVKVGDFVEIKNSKIAAGAKVPHLSYIGDAQVGKAANIGAGTITCNYDGSSKHPTIIGDHSFIGSNTNLVAPVEVGDYAVTGAGSTITKDVPAKALAVERSQQRNIANWKRPVKMKK"
  },
  {
    "reference": "MSWFGFFDPDFDDFFGRPRKYATEVPPNFNPRKIAQGDNGKGQQVSRYGAGAGHPHRALARRDDFFDDFWKNFSSGKYFVGFDDNVKTTEESDKYVVSYDQENLSPDEVNVDFDKQENELIITVTQETEKDGTKKSSTFHSNLKFEKPVNFDDISAEIGEQGVQVTLPKVHADHEKIVNIPISKAAAKK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be able to modulate filamentous growth of a population of unicellular organisms, cellular response to oxidative stress, cellular heat acclimation in a way that leads to a desirable outcome.2. The protein must exhibit the following characteristics: Heat shock protein required for pathogenicity; Plays a major role in trehalose homeostasis in response to elevated temperatures. The designed protein sequence is ",
    "response#1": "MKNNPVLIGEPGVGKTAIIEGLAQRIFSKDVPESLINCRIIELDMGALIAGAKYRGEFEERLKAVLSEIKESSGEIILFIDELHLLVGTGKTDGAMDASNLLKPMLARGELHCIGATTLDEYRKYIEKDAALARRFQPVYVSEPTVL",
    "response#2": "NKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVANTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAALERRFQPVMVQEPSIL",
    "response#3": "NKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVANTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAALERRFQPVMVQEPSIA"
  },
  {
    "reference": "SDRKDDGEEGDEGDEGGERDEEEERAEDKVDDRSKSGPETAMGQPGADNTPEVLNKALSGMSSRWRNWWVRGILSLAMIGFFFIIIYLGPLVLMLIVLGVQIKCFHEIITIGYNVYHSYELPWFRTLSWYFLLCVNYFFYGETVADYFFSLVQREEPLRLLFRYHRFISFTLYLTGFCMFVLSLVKKQYRLQFYMFGWTHVTLLIVVTQSHLVIQNLFEGMIWFIVPISMVICNDIMAYMFGFFFGRTPLIKLSPKKTWEGFIGGFFATVVFGLILSYLMAGIPYFVCPVAYNSETSRFTVDCHPSELFHLHEYSVPAVVQSVIGWKTVRLYPFQIHSIALSTFASLMGPFGGFFASGFKRAFKIKDFADTIPGHGGIMDRFDCQYLMATFVHVYMASFIKGPNPAKLLQQLLVLRPDQQIQIFQALRDSLMGKGLIQKEDD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in positive regulation of ERK1 and ERK2 cascade, CDP-diacylglycerol biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.2. Ideal protein should have a binding affinity for key enzymes in the CDP- diacylglycerol from sn-glycerol 3-phosphate: step 3/3, Phospholipid metabolism pathway to enhance the overall efficiency of the pathway.3. Prioritize Acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "LVFLPVHKSHIDYLLITFILFCHNIKAPHIAAGNNLNIPILSTLIRKLGGFFIRRKMDETSDGKKDVLYRSLLHAYTEELLRQQQFLEVYLEGTRSRSGKPSPARAGMLSIVVDTLCASTIPDVLIVPVGISYR",
    "response#2": "YQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLRNLGQGYVNFGEPMPLMTYLTYAKELRGATKEKESLPQMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNIAADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDSTVPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMAAIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQDDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLSVLHGINAPEFFDKAVFSSLVLTLRDEGVL",
    "response#3": "LVYAPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYATIFREYLGELFARGYSVEYFMEGGRSRTGRLLDPKTGTLSMTLQALLRGESRPITIIPIYIGYF"
  },
  {
    "reference": "AFRYFCKMIFYRLWIFTVCILVIIISIPRGRRCENMKFLSMAILPVKYIFGIKIVVKGKENLGTKKPFVLVSNHQTSLDVMVIMQILPDRCVPVAKKEILYMGSFGLACWLSGTIFIDRKKREESIAILTEAAHSMHKDNFRVLIFPEGTRNHDGSMLPFKRGAFQLAVKAQVPIIPAVISSYSSYYSQKEKRFTPGTCTIQILPAVETLGLGPGDVPKLTEQVRDSMLSTFQKIS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein contains novel Phospholipid/glycerol acyltransferase domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "RMRVRGSEQLQLRGPFVIVCNHQASLDLMGMVEVIPERCVPMAKRELQFLGTVGWACWLSGTIFIDRRRRDAAIEVIARTARALRRDNLRVWVFPEGTRNPGKSMLPFKRGAFHLAVQAQVPIVPVVIY",
    "response#2": "RLAFFCAGFLIKVKGKKATREEAPIFVVAPHSTFFDAIAVIVAGLPSVVSDTQHVRIPLVGQCILLTQPVLVRREDPNSRKTTRNEILSRVKSKMKWPQILIFPEGLCTNRSCLVTFKLGAFSPGVPVQPVLLRYPNTLDTVTWTWHGFSGFQVCMLTLSQPFTRMEVEFMPVYIPNEDEKKDPILFANTVRINMANALKLPVTDHSFEDCR",
    "response#3": "GLKIVVKGKEILRTKKPFVLVLNHQTSLDIMVMTEVLQDHCVAVAKKEILYMGTFGPACWLSGYIFIDRKKREESIATLTEVAHSLHKGNFSVMIFPEGTRNHGGSMLPFKYGAFHLAVKAQVPIIPAVN"
  },
  {
    "reference": "MNGIGARRIGLLGGTFNPVHNGHLRAALEVAEFMALDELRLIPSARPPHRDAPQASAEQRLAMVRLAVADEPRLTVDDRELQRDKPSYTVDTLESVRAELAADDQLFLLLGWDAFCGLPSWHRWQELLDHCHLLVLQRPDADSEAPEALRDLLAARSVSDPLSLAGPGGQISFIWQTPLAISATQIRHLLATGRSARFLLPDAVLAYIQAHDLYRAPNA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. We need a protein that can perform ATP + H(+) + nicotinate beta-D-ribonucleotide = deamido-NAD(+)   + diphosphate with high efficiency.2. The protein design should aim to mimic or enhance the function of natural proteins involved in NAD biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.3. The protein must exhibit the following characteristics: Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD)..4. We require a protein that can specifically bind to the metabolites produced in the Cofactor biosynthesis, deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1, NAD(+) biosynthesis pathway. The designed protein sequence is ",
    "response#1": "LFGGTFDPIHYGHLRPVEALAAEAGLNRVTLLPNHVPPHRPQPEANAQQRLKMVELAIAGNPLFAVDDRELHRTTPSYTIETLEAIRKERGAALPLAFIIGQDSLLTLHKWHRWQSLLDTCHLLVLARPGYNDRMDTPELQQWLEQHQVTDAALLSRQPQGYIYLADTPQLEISATEIRQQAA",
    "response#2": "LFGGTFNPLHNGHLQLAEFAAAQCQLDQVVFLPAASPPHKKGDEIVPFSHRAEMIRLACSRNKRFSCNTIEQDLARPSYTVDTLQALKTSPLYKSEAQFFFLIGVDAFIELKTWKAYRDLLSEINFILCPRKLFSRTQTVLFLTELGFVQTPLGWEHSSYLTLYELEGAPDQVSSTEVRPSQLFEYVELFFGTFNPIHTGHLIIANHMAEYSDLEEIWLVVTPHNPHKKKSSLLDNHHRLEMVYRACEGYGKLKPSNIEFDLPQPNYTVNTLAHIQEKFPTNDFCLIMGEDNLKSFHKWKNSEVIIENHEIYVYPRIAPGKVADEFKTHAKITRVAAPIIEISSTFIRDPAVIAAAKQEG",
    "response#3": "MPIFGGTFNPVHRGHLAMARAARDRCGLDQILWVPAAQPPHKPLAGGASIGDRVEMVRLAIAGEAGMALSLVDARRPGPSYAIDTLRLLEEQYPQAQWHWLLGQDGLADLPGWYRAAELIPRCRWIVVPRPGSGADPKQAMADLTERFGAVFVPLSDFECDISSTRVREAEGWLAQQR"
  },
  {
    "reference": "MAPPMTNCLTFSLSPMEMLKSTDQSHFSSSYDDSSTPYLIDNFYAFKEEAEIEAAAASMADSTTLSTFFDHSQTQIPKLEDFLGDSFVRYSDNQTETQDSSSLTPFYDPRHRTVAEGVTGFFSDHHQPDFKTINSGPEIFDDSTTSNIGGTHLSSHVVESSTTAKLGFNGDCTTTGGVLSLGVNNTSDQPLSCNNGERGGNSNKKKTVSKKETSDDSKKKIVETLGQRTSIYRGVTRHRWTGRYEAHLWDNSCRREGQARKGRQVYLGGYDKEDRAARAYDLAALKYWGSTATTNFPVSSYSKELEEMNHMTKQEFIASLRRKSSGFSRGASIYRGVTRHHQQGRWQARIGRVAGNKDLYLGTFATEEEAAEAYDIAAIKFRGINAVTNFEMNRYDIEAVMNSSLPVGGAAAKRHKLKLALESPSSSSSDHNLQQQQLLPSSSPSDQNPNSIPCGIPFEPSVLYYHQNFFQHYPLVSDSTIQAPMNQAEFFLWPNQSY",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design must be able to perform DNA binding in order to contribute to phyllotactic patterning. The designed protein sequence is ",
    "response#1": "PMLGVVTAGEPILAVEEASDFFPLPPDLRTEENALFMLTIRGESMINAGILDGDQVIVRKQSNANNGDIVIAMTDEDEATCKRFFREVDHIRLQPENDALAPILLDNVTILGKVIG",
    "response#2": "RSPGIIFEHDGGKTHTSRKVLYSCRVIPMRGSWLDFDFDHKDILYVRIDRRRKMPATILFKAMGMSKEQILDYFYTQETYRLEERNTIFWEVRKELYRKDNAYADIVC",
    "response#3": "LLNLQIDSYEKFLQEGVPEAERRPDEGLEGVFHTVFPIEDFNKTASLEFVSYEIGEPKYDQAECISKGLTYEAPMRIKVRLVVYDADEASGNRTIRDIKEQDIYFGTLPLMTEKGTFIINGTERVIVNQLQRSPGIIFEHDGGKTHTSRKVLYSCRVIPMRGSWLDFDFDHKDILYVRIDRRRKMPATILFKAMGMSKEQILDYFYTQETYRLEERNTIFWEVRKELYRKDNAYADIVDSEGNVIVKAGKPITKRSWRLICEAGIEAIEMRPDTLDGMFLAGDVADPKTGELLAEAADEITPGLLDRMRDAGINRVSVLHTKGNDTSSSIRDTLMLDRIPDQLKAQEEIYRRLRPSSPPTAEIAASFFDNLFRNPDYYDLSPVGRYKLNQRLSLDEPSDLRTLTDNDILTAIKVLVHLKDSHGPADDIDHLGNRRVRLVGELVENQYRIGLVRMERAIKERMSLQEISTLMPHDLINPKPI"
  },
  {
    "reference": "MIVLAAGAGTRMRSDIPKVLHPLAGRSMLEHAIRAGAGVSPEHLVTILGKSREVIAPAVTRLADALGRQIDVAVQDQQLGTGHAVLCGLTALPDSFDGTVVVTSGDVPLLDAETLTALIETHTAATSAATILTTTQSDPTGYGRVLRTQDNEVIAIVEQADATPSQQAICEVNAGVYAFDAGALRSALQRLRSDNAQGELYLTDVISILRGDGQVVLAEHVDDTMLVAGVNDRVQLSALGAELNRRIVATHQRAGVTVVDPASTWIDIEVTIGQDTVVAPGTQLKGSTAIGTHCAIGPDTTLDDVTVGDRASVVRTHGSASTIGAGATVGPFTYLRPGSHLGADGKLGAFVETKNATIGTGTKVPHLTYVGDADIGEHSNIGASSVFVNYDGQTKRRTTIGSHVRTGSDTMFVAPVTVGDGAYTGAGTVLREDVPPGALAVSAGPQRNIEGWVQRKRPDSASARAAEAAQTDTEEDA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a binding site that is specific to the target Mg(2+) ligand.2. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in cell morphogenesis, regulation of cell shape. The designed protein sequence is ",
    "response#1": "VTGTNGKTTVATLLYNLFKKAGFKVGLLSTVKVLVDDQEFPATHTTPDSLTINTYLRAMVENGVTHCFMEVSSHGIHQKRTKGLHFTGGIFTNLSHDHLDYHKTFAEYRDIKKSFFDELPKTAFALVNIDDKNGNVMLQNCEAKKYSYALKTVADYKAKILENQLGGLLLTINTHEVWSKLIGTFNAYNLLAIYATVDELRG",
    "response#2": "NALVRRLSACETMGSVTAICTDKTGTLTLNQMKVTEFWVGADRPRSAAAVNGGVVRLLCQGAGLNTTGSVYKPDNVSPPEITGSPTEKALLSWAVEELPMDADALKRKCKVVRVEAFNSDKKRSGVMLRDAATGAVTAHWKGAAEMVLARCTVYVGADGAARELGVEQRRKLEQVINDMAAASLRCIAFAYKQVVDGGDSDNAKIDDEGLTLLGFVGLKDPCRPEVKSAIEACTKAGIAVKMVTGDNVLTARAIAKECGIISGNDDDAAGVVIEGHEFRAMSEQEQLAIVDNIRVMARSLPLDKLVLVQRLKQKGHVVAVTGDGTNDAPF",
    "response#3": "EVAFVGRSNVGKSSLINSLTNRKKLAKVSQTPGKTRLVNFFLINDDFYLVDLPGYGYAKVSKAEKDSWGKTVEMYLTGREQLKRVVLLVDSRHKPTGDDIIMHEWIKHFGYDVIVVATKSDKLTRNELKKNEKVIKETLKLNSDDKLYFFSSLNKDGKDELIDNLI"
  },
  {
    "reference": "MFNRSVLREKNLSWMEPKALAYSIGELQLSFKLVSNNNDDSDTLTSFHLWTMRLMNNLEYESLKSGMYRETVEDSLEFEDVLDEGDLLDVLKIDDDAVEDMDLDVGLPGETVSDDEEEDSDDYDDDDDDDEREEEDDDEEDEDDLILDNELLDPIGALYALDTMSISSGDSVKSTAASVCISETPSLYSLYSEDRGISGNLSALSEFHLYNRMETSPTGHHDSSPTYDSSPSLSQDMDALRDEDDKKLLSRRRKRIPQRKRSIHTFEVSKREHSMGIVLLTLNKIKDLPVYRKAVSKASYDMDPFVIITFGRRVFKSSYRKRTLNPEYDEVCAFEIYPDERHFEFRFEVVDKDSFSFHDKVASASISWADVIKKYSIGETLDLEMPLELRKPADNNKPVMSISFQFLPYADLKRRFWTQSLHSLTTQKSFDVVELGVLLEKVGQFTCNEINEFFYYFNKVPWSFETLSLNELVAYLENWTNNNRYSSPFNNIQTCPLCGNRVMKSRNSVKSKLNRENDLISHFAICQLKRNQSQRTFLTPSYVSVKSASRRWFSKILIKMTYGKYALGSNNANILVQDRDSGVIIEEKISAHVKLGIRIIYNGKGVKSRKFKNLLRKQTIKQGRKFDAPSSVRYIPSFIRFHSLDMSECLKTEYKTFNEFFYRKLKPGSREPESQNPGILLSPADCRATVFPTVHKAQEIWIKGRQFTLRKLLSGCPQQSQFTENLSSIAIFRLAPQDYHRFHSPCEGVIGKIHYISGEYYTVNPMAV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.2. The protein must exhibit the following characteristics: Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine..3. The protein should have Helical domains to ensure proper insertion into the membrane.4. The designed protein must have at least one C2 domain.5. The protein should be engineered to have a higher affinity for a specific molecule or receptor in endosome membrane that is critical for protein autoprocessing.6. The designed protein must have a Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "LRRKSMAARAYNFLGAVNDVNGLIFMEIVKINDLPPERNMTRTSFDMDPFVVAALGRKVLRTRVVRHNLNPVFNEKMVFQILQHETGYSLTFTVIDRDSFSGNDFVASTSFPISKMIEAAPEEDPLRANVIKGRDLAAKDRSGTSDPYLVLSLGDAKATTPTINKQLNPEWNETLELPIVGEQSLLLEVVCWDKDRFGKDYMGEFDVILEDQFQNGLTQQEPQWFPL",
    "response#2": "IVMLEIKAAEDLPKLRNITRTGWDMDPFCIIAFGRKIFRTRVIRHSLNPTWDEKLLFHVRRYETSYKLAFTVCDWDKLSSNDHVGEALLELSELLQGCPKPDE",
    "response#3": "LKLYVIKVLDLPSVDMAQIPNSFAGTKPGCKRQYLIVTLGNSKQSTPSIPKTLNPEWKICFDMPLTGVPLLECICWDKDRFGKDYMGEFDLPIEDIFANGQVQQEPKWYKR"
  },
  {
    "reference": "MRSADQFTIANKPIASIDLMEKAARAFVQHFLRNEFDTNKSVAIFCGKGNNGGDGLAIAHLLIANGYENIKVYIINFSKKQSDDFAINLQRIEETRCKKTIINQASDLKNLKADLIIDAILGSGLNKPLSGEFEQLVQAINKLNKKVYAVDVPTGFFAEGKLPKHYNGIKAYKTICFQRPKISFFFPESALATEKYEVVDIDLDEAFIQKQDSDFYLVEESDIENILQPRKLFSHKGTYGHALIIAGNTNTMGAALLSSMACLHAGAGLTTACIPSSGLIALNTSLPEVMALPRDEYTRIENPKKYQAIAIGPGLATGPENEKLLESLILANQAMIIDADAVNILGERPDLIDKLAANTIITPHMKEFDRLFGEHDSWWDRVQTAKEQAQKLKMVIVLKNQYTFICLPTGKVLINPTGNPAMAQGGMGDILTGIIAGFIAQQYSATDAAVLACYIHGKTGDYLAHEHFVVTASQVAERVSKEIKLLISK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design must be able to perform ATP binding, metal ion binding, NADHX epimerase activity in order to contribute to nicotinamide nucleotide metabolic process.2. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.3. The (6S)-NADPHX binding site should be located in a region of the protein that is accessible to the ligand.4. For general function, the protein need meet that Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. The designed protein sequence is ",
    "response#1": "MFTPLYTADEMRAAEEGHDVDAMMERAGRAVADAVLRRYPDARRIAGVCGRGANGGDGRIALRLLAEAGRETSEELDGADVVIDALFGTGFQGVPRDDAASSIERINSSRAPVVAVDLPSGVDASTGEVAGAVVDADVTVTMHASKVGLAIAPGRFHAGDVDVADIGIRPEDVAALLPRPAPDAHKYTRGVVGIRAGSDQYPGAALLSVAGANSGLVGMVRYVGAAADRVREVHPEVVGAGRVQAWLVGSGGGEHAGEELRAALEDGVPVVVDADALAHVTGPLPAPAVLTPHAGELAAMTGATRAEIEAAPLRHAQQAAATYGCTVLLKGRHTLVATAGRPVRATTTGTAWLATAGAGDVLGGLVAALLAAGLDPHDAASVGSWLHGAAATLAGGGGPIVASDVAHAIPDAVRGL",
    "response#2": "GIRAGSDQYPGAALLSVAGANSGLVGMVRYVGAAADRVREVHPEVVGAGRVQAWLVGSGGGEHAGEELRAALEDGVPVVVDADALAHVTGPLPAPAVLTPHAGELAAMTGATRAEIEAAPLRHAQQAAATYGCTVLLKGRHTLVATAGRPVRATTTGTAWLATAGAGDVLGGLVAALLAAGLDPHDAASVGSWLHGAAATLF",
    "response#3": "MFTPLYTADEMRAAEEGHDVDAMMERAGRAVADAVLRRYPDARRIAGVCGRGANGGDGRIALRLLAEAGRETSEELDGADVVIDALFGTGFQGVPRDDAASSIERINSSRAPVVAVDLPSGVDASTGEVAGAVVDADVTVTMHASKVGLAIAPGRFHAGDVDVADIGIDDDVADLLPRPEAESDKYRRGVLGIVAGSDRFTGAAVLAVGGAIRAGAGLVRFASAETAVRQVRNRWPEAITTVLDGPSVAEVGRVQAWVLGPGMGTGEEAVALARAVLATDVPVLVDADGLTILANDRSLLRRSAPTVLTPHAGELSRLLDVPRERIEAARMEHVRRAVAELGAIVLLKGSTTLIAEQDRPVRVNTTGTPWLATGGTGDVLSGIAGTLLSGGLSGYDAVSCAAHLHGLAAQAASTGISGGHGPIAAMDVATMLPQVIGMTGAALLAASAALHAGAGRVMVSLLDDGEVTQDIAQPECMLRRFDALPLHELTAVCGCGGGQAVRAVLPTVMAQAMRLVLDADALNAIAADTSLQHALRSRAASGLPTVLTPHPLEAARMLGADAAAVQADRLAAIRQLVDRFECAVVLKGSGSIVAAPGHAPRINRTGNARLATGGTGDVLAGLVGALLARMDSANAQMALDAALLACEHHGQVADDWPAGRAVLVVEGPMTGAALLACAGAMHAGAGAVRLSTERVHPAVPPDVVLRAEPLGDLLGDRRTGAVVAGPGLGRDAAARERLSAVLSAGRPSVIDADALALLRPGDLERFDAPLVLTPHAGEMASLAESFGIAAIGKVEQARALARAARAVVVFKGPDTVIAGPDGSVGFLPTATSWLSVGGSGDVLGGVVAARLAATGDPFQAASEGAWLHAEAARLAGPA"
  },
  {
    "reference": "MSIQETNSRSLSDDLGAIHYGSTVSRTTDVTHDSRQVREGTLFVAIKGATVDGHRFIEDVMRRGAAGIISEYDPPEAFEGIWLKVSDARVALAKSASIINGHPSHKLDLVGVTGTNGKTTTTYLCYALAEASGVKSAMLTTVEYRIGERSEEAIRTTPEASDTNRFLRHAVDEGCGFAAMEASSQAIDLHRCDWLRFKIAVFTNLTQDHLDYHLTMENYFDAKKKLFDGRLGEKPASSVINIDDEWGSRLADELKAAGQRVVTISQDAARRFADLTAENVMVSLLKGTSFLLKTPEGERTVISPLVGRPHVYNMLAASATALELGYSLEDIVNGLSKCIGAPGRFERVQHDGDFAVVVDYAHTDDALLNTLRTAKELTKGRVITVFGCGGDRDKTKRKPMGRAAGSHSDIAIITSDNPRTEDPLKIMDEIESGLKETGTEYLSISDRREAINKAISLASPGDVVIIAGKGHENYQIIGSDKFHFDDREVALAALEALKEI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should be able to enhance the efficiency of cell wall organization.2. A protein that can modulate the overall flux of metabolites through the peptidoglycan biosynthesis, Cell wall biogenesis pathway, enhancing or suppressing its activity as needed.3. The designed protein should be able to compete with endogenous proteins or inhibitors for meso-2,6-diaminoheptanedioate binding. The designed protein sequence is ",
    "response#1": "VTGTNGKTTTSFILQHLINEAKGDCGLIGTVKYLTGGGQEEPASHTTPDSITLQRLLRAMTENSCVAASMEVSSHALQQRRTHSIRFDAAIFTNLTQDHLDYHGTMEDYYQAKALIASQMVEQGGPKKPALILNTDDAAGRRLAREYEGKLRIVTFGANVHADFRAAQITTTMKGTTFTLEARGRSFMVRTPFIGRFNVYNAVGALAAVLDALGVGREEALGALSTFPGVPGRVQRIDRGQDFLAVVDYAHKPAAVEAVLATLRESSRGRIAVVLGAGGDRDTAKRPLMGAAGARGADLLVVTDDNPRSEDPAAIRHAVEDGALSVPDDARGEVRNVEGRAAAIEQAVAWAREGDVVLIAGKGHETGQIVGDRVLPFSDHAEVAAALRAR",
    "response#2": "VTGTSGKSTSTFLLEAGLRAAGHSAGLIGGVEIHVAGRSFPAQLTTPEASDVHGLLALMREEGVTAAAMEVSSHALALDRVDGVRYDVAVFTNLSQDHLDFHRDLDDYFATKARLFTPEFSRVGVVNLDDAHGRELLGMAKIPMTSFSAAGSARAEWRAQDVRCGGDGSTFRVVGPGGVEADVTVSLPGPFNVSNTLGAIVR",
    "response#3": "VTGTNGKTTISRLVAELLMLQGQKCAVMGTTGNGILPQLEASSHTTLDALHLQNLLHDFAQQGAEFVSLEASSHGLEQGRLNGCNIEIAAYSNLSRDHLDYHGTLEAYAEAKSRLFKFSSLKVAVINIDDTYANIMLNAASSNLAHPKVLTYSTSKAADYQVFGIKYSLEGASFHLKTVRGEFTVNSPLLGHFNIENLVASLIVALGRRFTLTGEWNGASIFDDYGHHPVEIKAVLRAAREACQGRIVAVHQPHRYSRLSSLFEDFSACFNDADTILIAPVYAAGEDAIDGVNSQALVNSIKAAGHRDAQFLTGPEALAPVVSKIAQPGDFVVLLGADMSDVKNIMNKLGT"
  },
  {
    "reference": "MVIILLLLLLSFLDPSLEESLAPRLTRTPRAPCARGQACDPRQRRDAGGRGGVYEHLGGAPRRRKLYCATKYHLQIHPNGKIDGSLEENNPLSILEITAVDVGVVAIKGLFSGRYLAMNEKGRLYASEVFNRECEFLERIHELGYNTYASRHHATTQPPPTGSGIGGSKRRASSKRQWYVSINGKGRPRRGFKTRSTDKASLFLPRVLANKDHEMVRKLRESQRHHTGSHRAPVGRAERRRRRHRGSKGHNRRADI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The ideal protein would be one that plays a critical role in animal organ morphogenesis, locus ceruleus development through growth factor activity within extracellular matrix, cytoplasm. The designed protein sequence is ",
    "response#1": "DCSYTISQVRSMKILKRFGGSAGLWTKDPLGPAEKIYVLDGTQNDTAFVFPRLRDFTLTMAARKASRIRVPFPWVGTGQLVYGGFLYYARRPPGGAGGGGELENTLQLIKFHLANRTVVDSSVFPAERLIPPYGLTVDTYIDLAADEEGLWAVYATREDDRHLCLAKLDPQTLDTEQQWDTPCPRENAEAAFVICGTLYVVYNTRPASRARIQCSFDASGTLTPERAALSYFPRRYGAHASLRYNPRERQLYAWDDGYQIVYKLEMKC",
    "response#2": "CRRHPLYVDFSDVGWNEWIVAPPGYHAFYCNGECPFPLSDHLNSTNHAIVQTLVNSVNSNIPRACCVPTELSPISLLYLDEYEKVVLKNYQDMVVEGCGCS",
    "response#3": "RNTITTKDLAPGYNDLLLLPEGATNIRIEETVPSSNYLACRNHSGHYYLNGDWRIDFPRPMFFANSWWNYQRKPMGFAAPDQLTCSGPISESLFIVMLVQEKNISLDYEYSIG"
  },
  {
    "reference": "MEPVDPNIEPWNQPGSQPKTACNQCYCKKCCWHCQLCFLKKGLGISYGRKKRRQRRAASRSSKNHQDHIPEQRSLPQTRRVSTGSEESKEKVESKTEPDRFD",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein with cyclin binding functions is required to enable suppression by virus of host type I interferon-mediated signaling pathway.2. For general function, the protein need meet that Besides its effect on RNA Pol II processivity, Tat induces chromatin remodeling of proviral genes by recruiting the histone acetyltransferases (HATs) CREBBP, EP300 and PCAF to the chromatin; Other factors such as HTATSF1/Tat-SF1, SUPT5H/SPT5, and HTATIP2 are also important for Tat's function; Tat can reactivate a latently infected cell by penetrating in it and transactivating its LTR promoter; To ensure maximal activation of the LTR, Tat mediates nuclear translocation of NF-kappa-B by interacting with host RELA The designed protein sequence is ",
    "response#1": "MEPVDPNLEPWNHPGSQPKTACNKCYCKICCWHCQICFLKKGLGISYGRKKRKHRRGTPQSSKDHQNPIPKQPFPRTRGNPTGPKESKKEVASKAETDPCD",
    "response#2": "ECFAXLPXG",
    "response#3": "ECFHIFSFTGEEMATKADYTLDEESRARIKTRL"
  },
  {
    "reference": "MASVSTHGNQEKSPHLPPLSKQSLLFCPKSKLHIHRGEIAKIIRECQEESFWKRALPFSLISMLVTQGLVHQGYLAANPRFGSLPKVALAGLLGFGLGKASYIRVCQSKFHSFEDQLRGAGFGPEHNRHCLLTCEDCKTRRGLSEKAGSQPSAS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be expressed specifically in the mitochondrial inner membrane2. The designed protein must have at least one OCIA domain. The designed protein sequence is ",
    "response#1": "MRQIQQKLRNVDCIVEIHDSRIPLAGRNSQFFDTITGSGVKPHILVLNKVDLLGPKQQRSVLQQLRRQQPELKNILFTNCKDQRNHGVLDILPLATQLVGDSSRYNRAQSAEHNIMIIGVPNVGKSSIINVLRNVHLKKRSAARVGAEAGVTRAVGERIKIQEKPAVYMIDTPGILQR",
    "response#2": "MRQIQQKLRNVDCIVEIHDSRIPLAGRNSQFFDTITGSGVKPHILVLNKVDLLGPKQQRSVLQQLRRQQPELKNILFTNCKDQRNHGVLDILPLATQLVGDSSRYNRAQSAEHNIMIIGVPNVGKSSIINVLRNVHLKKRSAARVGAEAGVTRAVGERIKIQEKPAVYMIDTPGILQR",
    "response#3": "MRQIQQKLRNVDCIVEIHDSRIPLAGRNSQFFDTITGSGVKPHILVLNKVDLLGPKQQRSVLQQLRRQQPELKNILFTNCKDQRNHGVLDILPLATQLVGDSSRYNRAQSAEHNIMIIGVPNVGKSSIINVLRNVHLKKRSAARVGAEAGVTRAVGERIKIQEKPAVYMIDTPGILQR"
  },
  {
    "reference": "MASADEKVVEEKASVISSLLDKAKGFFAEKLANIPTPEATVDDVDFKGVTRDGVDYHAKVSVKNPYSQSIPICQISYILKSATRTIASGTIPDPGSLVGSGTTVLDVPVKVAYSIAVSLMKDMCTDWDIDYQLDIGLTFDIPVVGDITIPVSTQGEIKLPSLRDFF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design should prioritize the formation of helix-turn-strand-strand-strand-strand-strand-strand-helix-strand-strand-strand-strand elements.2. The protein design must be focused on enhancing the efficiency of response to desiccation. The designed protein sequence is ",
    "response#1": "QKRTPLMQVPVLVDLNGNHLWVNCEQQYSSKTYQAPFCHSTQCSRANTHQCLSCPAASRPGCHKNTCGLMSTNPITQQTGLGELGEDVLAIHATQGSTQQLGPLVTVPQFLFSCAPSFLVQKGLPRNTQGVAGLGHAPISLPNQLASHFGLQRQFTTCLSRYPTSKGAIIFGDAPNNMRQFQNQDIFHDLAFTPLTITLQGEYNVRVNSIRINQHSVFPLNKISSTIVGSTSGGTMISTSTPHMVLQQSVYQAFTQVFAQQLPKQAQVKSVAPFGLCFNSNKINAYPSVDLVMDKPNGPVWRISGEDLMVQAQPGVTCLGVMNGGMQPRAEITLGARQLEENLVVFDLARSRVGFSTV",
    "response#2": "HWANLQKRTPLMQVPVLVDLNGNHLWVNCEQQYSSKTYQAPFCHSTQCSRANTHQCLSCPAASRPGCHKNTCGLMSTNPITQQTGLGELGEDVLAIHATQGSTQQLGPLVTVPQFLFSCAPSFLVQKGLPRNTQGVAGLGHAPISLPNQLASHFGLQRQFTTCLSRYPTSKGAIIFGDAPNNMRQFQNQDIFHDLAFTPLTITLQGEYNVRVNSIRINQHSVFPLNKISSTIVGSTSGGTMISTSTPHMVLQQSVYQAFTQVFAQQLPKQAQVKSVAPFGLCFNSNKINAYPSVDLVMDKPNGPVWRISGEDLMVQAQPGVTCLGVMNGGMQPRAEITLGARQLEENLVVFDLARSRVGFV",
    "response#3": "QKRTPLMQVPVLVDLNGNHLWVNCEQQYSSKTYQAPFCHSTQCSRANTHQCLSCPAASRPGCHKNTCGLMSTNPITQQTGLGELGEDVLAIHATQGSTQQLGPLVTVPQFLFSCAPSFLVQKGLPRNTQGVAGLGHAPISLPNQLASHFGLQRQFTTCLSRYPTSKGAIIFGDAPNNMRQFQNQDIFHDLAFTPLTITLQGEYNVRVNSIRINQHSVFPLNKISSTIVGSTSGGTMISTSTPHMVLQQSVYQAFTQVFAQQLPKQAQVKSVAPFGLCFNSNKINAYPSVDLVMDKPNGPVWRISGEDLMVQAQPGVTCLGVMNGGMQPRAEITLGARQLEENLVVFDLARSRVGFSTC"
  },
  {
    "reference": "MPGVCDRAPDFLSPSEDQVLRPALGSSVALNCTAWVVSGPHCSLPSVQWLKDGLPLGIGGHYSLHEYSWVKANLSEVLVSSVLGVNVTSTEVYGAFTCSIQNISFSSFTLQRAGPTSHVAAVLASLLVLLALLLAALLYVKCRLNVLLWYQDAYGEVEINDGKLYDAYVSYSDCPEDRKFVNFILKPQLERRRGYKLFLDDRDLLPRAEPSADLLVNLSRCRRLIVVLSDAFLSRAWCSHSFREGLCRLLELTRRPIFITFEGQRRDPAHPALRLLRQHRHLVTLLLWRPGSVTPSSDFWKEVQLALPRKVQYRPVEGDPQTQLQDDKDPMLILRGRVPEGRALDSEVDPDPEGDLGVRGPVFGEPSAPPHTSGVSLGESRSSEVDVSDLGSRNYSARTDFYCLVSKDDM",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should contain Cytoplasmic, Extracellular topological domains.2. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.3. The protein should have Helical; Signal-anchor for type III membrane protein domains to ensure proper insertion into the membrane.4. The protein must exhibit the following characteristics: Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways; Through its extracellular domain interferes with the heterodimerization of Il1R1 and IL1RAP..5. The protein should be able to modulate negative regulation of cytokine-mediated signaling pathway, negative regulation of chemokine production, signal transduction, negative regulation of DNA-binding transcription factor activity in a way that leads to a desirable outcome.6. The presence of TIR domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "VLRCPQKTDCALCVRVVVHLAVHGHWAEPEEAGKSDSELQESRNASLQAQVVLSFQAYPIARCALLEVQVPADLVQPGQSVGSAVFDCFEASLGAEVQIWSYTKPRYQKELNLTQQLPDCRGLEVRDSIQSCWVLPWLNVSTDGDNVLLTLDVSEEQDFSFLLYLRPVPDALKSLWYKNLTGPQNITLNHTDLVPCLCIQVWSLEPDSERVEFCPFREDPGAHRNLWHIARLRVLSPGVWQLDAPCCLPGKVTLCWQAPDQSPCQPLVPPVPQKNATVNEPQDFQLVAGHPNLCVQVSTWEKVQLQACLWADSLGPFKDDMLLVEMKTGLNNTSVCALEPSGCTPLPSMASTRAARLGEELLQDFRSHQCMQLWNDDNMGSLWACSHKQDLLLGSTGSISCPSLSCQSDAQSPAVTWYKNGKLLSVERSNRIVVDEVYDYHQGTYVCDYTQSDTVSSWTVRAVVQVV",
    "response#2": "VLRCPQKTDCALCVRVVVHLAVHGHWAEPEEAGKSDSELQESRNASLQAQVVLSFQAYPIARCALLEVQVPADLVQPGQSVGSAVFDCFEASLGAEVQIWSYTKPRYQKELNLTQQLPDCRGLEVRDSIQSCWVLPWLNVSTDGDNVLLTLDVSEEQDFSFLLYLRPVPDALKSLWYKNLTGPQNITLNHTDLVPCLCIQVWSLEPDSERVEFCPFREDPGAHRNLWHIARLRVLSPGVWQLDAPCCLPGKVTLCWQAPDQSPCQPLVPPVPQKNATVNEPQDFQLVAGHPNLCVQVSTWEKVQLQACLWADSLGPFKDDMLLVEMKTGLNNTSVCALEPSGCTPLPSMASTRAARLGEELLQDFRSHQCMQLWNDDNMGSLWACE",
    "response#3": "VLRCPQKTDCALCVRVVVHLAVHGHWAEPEEAGKSDSELQESRNASLQAQVVLSFQAYPIARCALLEVQVPADLVQPGQSVGSAVFDCFEASLGAEVQIWSYTKPRYQKELNLTQQLPDCRGLEVRDSIQSCWVLPWLNVSTDGDNVLLTLDVSEEQDFSFLLYLRPVPDALKSLWYKNLTGPQNITLNHTDLVPCLCIQVWSLEPDSERVEFCPFREDPGAHRNLWHIARLRVLSPGVWQLDAPCCLPGKVTLCWQAPDQSPCQPLVPPVPQKNATVNEPQDFQLVAGHPNLCVQVSTWEKVQLQACLWADSLGPFKDDMLLVEMKTGLNNTSVCALEPSGCTPLPSMASTRAARLGEELLQDFRSHQCMQLWNDDNMGSLWACSHKQDLLLGSTGSISCPSLSCQSDAQSPAVTWYKNGKLLSVERSNRIVVDEVYDYHQGTYVCDYTQSDTVSSWTVRAVVQVSVQVLRCPQKTDCALCVRVVVHLAVHGHWAEPEEAGKSDSELQESRNASLQAQVVLSFQAYPIARCALLEVQVPADLVQPGQSVGSAVFDCFEASLGAEVQIWSYTKPRYQKELNLTQQLPDCRGLEVRDSIQSCWVLPWLNVSTDGDNVLLTLDVSEEQDFSFLLYLRPVPDALKSLWYKNLTGPQNITLNHTDLVPCLCIQVWSLEPDSERVEFCPFREDPGAHRNLWHIARLRVLSPGVWQLDAPCCLPGKVTLCWQAPDQSPCQPLVPPVPQKNATVNEPQDFQLVAGHPNLCVQVSTWEKVQLQACLWADSLGPFKDDMLLVEMKTGLNNTSVCALEPSGCTPLPSMASTRAARLGEELLQDFRSHQCMQLWNDDNMGSLWAC"
  },
  {
    "reference": "MGSLLRASKALKPPKYLSPFFSTFRSNNAYDSFGLTQRFCSHSRQNSKETATIDLSQYPTEKIRNFSIIAHVDHGKSTLADRLLELTGTIKRGHGQPQYLDKLQVERERGITVKAQTATMFHKHKFHGSSVGDANEPSTFLLNLIDTPGHVDFSYEVSRSLAACQGALLVVDAAQGVQAQTVANFYLAFESDLTIIPIINKIDQPTADPDRVKAQLKSMFDLEPTDALLTSAKTGQGLEHVLPAVIERIPPPPGSSNSPLRMLLLDSYYDEYKGVICHVAIVDGALRKGDKISSAATGQAYDVLDVGIMHPELTPTGILLSGQVGYVVTGMRSTREARVGDTLYHTRTTVEPLPGFKPAKHMVFSGLYPADGSDFDALNHAIERLTCNDASVSITKESSTALGLGFRCGFLGLLHMDVFHQRLEQEYGAHVISTVPTVPYIFEYSDGSKVEVQNPAALPSNPKKRVTACWEPTVIATIIIPSEYVGPVITLCSERRGQQLEYSFIDSQRAFMKYQLPLREIVVDFYNELKSITSGYASFDYEDSKYQQSDLVKLDILLNGQPVDAMATIVHNLKAQRVGRELVDKLKKFIDRQMFEITIQAAIGSKVIARETISAMRKNVLAKCYGGDVTRKRKLLEKQKEGKKRMKRVGSVDIPQEAFHQLLKALWHACAGPLVAVPIEGERVFYFPQGHLERVEASTNQVIDLPRNDLPWKILCRVINVQLKAEADTDEVFAQVTVLPEPNQDENGAEMEPPLPPLPELLEHSFCK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. We need a protein that can perform GTP + H2O = GDP + H(+) + phosphate with high efficiency.2. The designed protein must be able to perform ribosome binding under specific conditions. The designed protein sequence is ",
    "response#1": "NGVLISNSTGMAVAFALFNLQERGKLFLGHGTQVYEGQIIGLHNRSNDLTVNCLTGKKLTNMRASGTDEAIILTTFTKFTLEEALSFINDDELVEITPKSIRLRKRYLG",
    "response#2": "HIRNFSIIAHIDHGKSTLADRIIQLCGGLSDREMEAQVLDSMDIEKERGITIKAQTAALSYKARDGQVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVEVVPVLNKIDLPQADPDNAIQEIEDVIGIDAQDATPCSAKTGQGVQDVIEALIAKVADVGFVGKPSAGKSTLLSVISNAKPTIADYDFTTLVPQLGLVKYFDNSFVVADLPGLIKGAHQGKGLGIRFLKHIERCKVIANVIDFGDENKNPLQDYQEIRNELKLYNLNLEEKDEVIVANKKDQECFEKKLEEFSKHYPNKKIIAISALKQENLDKLKEALWQSVL",
    "response#3": "NRPQYSDDAGYDLYLQEDLMLFPQASTTVTIDSRVPTTTKFFKPVVFGRSGLATRGVVVDVVKWTHSPLTLKIYNFTDNTLRYSAGTRICQVVFVHRRHFPK"
  },
  {
    "reference": "MSPVQDELIVTVNAGSSSIKTGLFSGRRGEDQPRLLARGKLDGIGVAPRLQVVMADGTIMQDTSFEPEQIANMQAAFQQIYRVLEEGLQDRPPVLIGHRVVHGGMDFSAPVRVDHTTLARLKTLEPLAPLHQPHNLAAIHAALEHDPQLPQIACFDTAFHAGRNEISQLFGLPYALYEQGVRRYGFHGLSFEYVSQRLIDKTPELATGKLVIAHLGNGSSLCAIEAGRSIEMTTGFSALDGLPMGSRCGAVDPGVLLYLLGQGISVDELEKLLYRQSGLLGISGVSSDMRKLRASNEPRAALAIDYYAYRIAQEVGRLATCLGGLDALVFTAGIGEKDAELRAQVLQHLAPLFGLQLDDTANRRNAELISGTSSTRSVWVIPTDEESIIARHSWHLYQEQHP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be able to bind ATP ligand in a variety of conditions2. The designed protein must be able to perform magnesium ion binding, ATP binding, acetate kinase activity under specific conditions. The designed protein sequence is ",
    "response#1": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYH",
    "response#2": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#3": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR"
  },
  {
    "reference": "MSSNTHHWIARRGTALQGSLAIPGDKSVSHRAVMFAALADGVSQIDGFLEGEDTRSTAAIFAKLGVRIETPSASQRIVHGVGVDGLQPPTGALDCGNAGTGMRLLAGLLAAQRFDSVLVGDESLSKRPMRRVTGPLAQMGARIDTQDDGTPPLRVHGGQALHGIDFVSPVASAQVKSAVLLAGLYAQGETSVTEPHPTRDYSERMLSAFGVDIDFSPGSARLRGGQRLRATDIAVPADFSSAAFFIVAASIVPDSEVVLRAVGLNPRRTGLLAALRLMGADISEENHAEHGGEPVADLRVRYAPLRGAQIPEALVPDMIDEFPALFVAATAASGQTVVTGAAELRVKESDRLAAMATGLRTLGVQVDETPDGATIHGGSIGSGVIESHGDHRIAMAFAIAGQLSSGSVRVNDVANVATSFPGFDTLAQGAGFGLEAAESG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Ideal protein should have a binding affinity for key enzymes in the Metabolic intermediate biosynthesis, chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7 pathway to enhance the overall efficiency of the pathway.2. The protein with the reaction 3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-   carboxyvinyl)-3-phosphoshikimate + phosphate, the Proton donor, Proton acceptor must be optimized to ensure efficient catalysis.3. The designed protein must possess 3-phosphoshikimate 1-carboxyvinyltransferase activity to support aromatic amino acid family biosynthetic process under certain conditions.4. The protein should have a high affinity for 3-phosphoshikimate. The designed protein sequence is ",
    "response#1": "SEVKKTYELPGDKSVGHRSLLIGALPKGEYKIRNFPQSRDCLTTLKIMEELGVKVKVLKDYILVNSPGYENFKKKIDYIDCGNSGTTSRLIAGILAGVGVETNLVGDKSLSIRPMKRIVDPLNSMGANIEMEKDHMPLIFKGNGELKGIDYTMEIASAQVKSCILLAGFLSEGVTKVRELSPTRDHTERMLKYIEGNIKIENKEIEIENSTIKSKDIYVPGDISSAAYIIACAILGEDCEIILENVLLNENRRKYLDLLKKMGANLKYLEKNQCNGEHVGNILVKSSFLKGISIGKEITPYIIDEIPIISLIASFAEGKTIFENVEELKYKESDRIKAIMVNLKSLGVKTELVENNLIIYGGLSKINKEINIRTFNDHRIALTFLCSAMRNSEKTYIDNWDCVAISFPTD",
    "response#2": "PGRPLSGRAMPPGSKSITNRALLLAGLAKGTSRMTGALKSDDTRYMADALRAMGVAIDEPDDTTFVVTGSGRLMPPKAPLFLGNAGTATRFLTAAAALVDGTVVVDGDEHMRKRPIGPLVEAMRTLGIDVSADTGCPPVTVRGTGRFEADRILIDGGLSSQYVSALLMMAAGGDRPVDIELVGEDIGALGYIDLTTAAMKAFGARVEKTSPVTWRVEPTGYRAADFVIEPDASAATYLWAAEVLSDGQIDLGVPNDAFTQPDAKAYETIAKFPHLPAEIDGSQMQDAVPTIAVLAAFNETPVRFVGIANLRVKECDRIRALSTGLNNIREGLAVEEGDDLIVHSDPALAGQTLPAEIDTFADHRIAMSFALAGLKIDGITILDPDCVGKTFPAYWRTDATLPMPGSKSHANRAIITACLAKGTTILRNATPCDDVAVLVANVRKMGFRAEWIDQSQGILKIIGGIPARVGARHALPLHAVTLDCGLAGTTLRFLTALCSITPGEWIVTGNARMQKRPIGDLTRALRMLGAEIEDTKVSAETTASPLTSCPPLRISGKTLDGGNVILDASKSSQFLSALLLIAPSMDDGLTLTLSSGLASEPYVDLTMKTLKQFGVNVRKNGRNFRVTHQSLRSPPAITVEGDWSAAGAFLVLEEITGSRINAPNLDAHSTQADAKLPDVIRKLRGKGKMTINCAGFPDQVMNIAVLAAHRKGETILTGAANLRLKESDRLGVLTQELRKAGIRIKERPDGLRILHSPKLRPALLDPHDDHRMAMAFAILGCLHPGIKILNSDCVRKSYPRFWKD",
    "response#3": "STCLSGKIKIPGDKSISHRSLILGGLANGETHIHGLLESDDVLNTAAAMQAMGACIIKKADRWIIRGTGNGCLLAAEKPLNFGNAGTGARLVMGMVGPYHMKTTFIGDASLSKRPMGRILNPLRLMGVEIEATHGDRLPLTLYGPKMANPIRYRIPIASAQVKSAILLAGLNTAGTTTVIEPILTRDHTEKMLKAFGAELEIKTDAEGTRFIHLNGQPHLTGQTIHIPGDPSSAAFPIIAALLVENSDITIENVLINKSRMGLIETLWEMDAKIELLNQHKTGGENVADLRVKSSMLKGVTVPKERAPSMIDEYPALAVAAAFAEGKTVMLGIDELRVKESDRLSALAQGLKINHVDCEEGKDFLIVHGKSSAKGLGGGHVTTHLDHRIAMSFLIFGLVSEKPVTIDDKRMIATSFPEFL"
  },
  {
    "reference": "MTRKILIVEDECAIREMIALFLSQKYYDVIEASDFKTAINKIKENPKLILLDWMLPGRSGIQFIQYIKKQESYAAIPIIMLTAKSTEEDCIACLNAGADDYITKPFSPQILLARIEAVWRRIYEQQSQFIQIDELSIDENAQRVFFQQQEINLSSTEFKLLHFFMRHPEKVYSREQLLNRIWHNDLEVEYRTVDSYIRRLRRNLAPFQCEHYIQTVRGSGYRFSSYLRDKQ",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that This protein is a positive regulator for the phosphate regulon; Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited .2. The designed protein should contain at least one OmpR/PhoB-type domain that can specifically recognize and bind to DNA.3. The designed protein should be involved in phosphate ion transport by localizing to protein-DNA complex, cytosol and exhibiting phosphorelay response regulator activity, DNA-binding transcription activator activity, transcription cis-regulatory region binding.4. The designed protein must have at least one Response regulatory domain. The designed protein sequence is ",
    "response#1": "MVGKSPAMQHLLSEIALVAPSEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQIISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVDQPVRLTATEAALMRIFAAHAGEVIGRTELIEELGRDRSASAEEAAGDRAVDVQITRLRRKIEPDPREPRYLQTVRGLGYMG",
    "response#2": "MVGKSPAMQHLLSEIALVAPSEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQIISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVAQ",
    "response#3": "MVGKSPAMQHLLSEIALVAPSEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQIISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVLPDAVVLTTEEGAIFWCNGLAQQILNLRWPDDSGQNILNLLRYPEFANYLKQRDFSKPLNLVLNNARHLEIRVMPYTDKQWLMVARDVTQMHQLEGARRNFFANVSRFRSGAESLPDAVVLTTEEGAIFWCNGLAQQILNLRWPDDSGQNILNLLRYPEFANYLKQRDFSKPLNLVLNNARHLEIRVMPYTDKQWLMVARDGPAECLPDAVVLTTEEGAIFWCNGLAQQILNLRWPDDSGQNILNLLRYPEFANYLKQRDFSKPLNLVLNNARHLEIRVMPYTDKQWLMVARDVTQMHQLEGARRNFFANVSGPLXCXYXAXGGXPXRXSXGXAX"
  },
  {
    "reference": "MTASPHRHELYTVEQVRALDRRAIDDLGVPGYELMQRAAAAALVCLRQHWPQARRIAVYGGPGNNGGDGFLLAALAREAGIHAEVFAWGESRGDDAVRARREFEHGGGTVHAWREGIELPLVDVHVDALYGTGLQRALTADIAGLVQCIHATGAPVLALDVPSGVNADTGDVPGVAVQADVTVTFIALKRGLFTGLAPGYVGTVQWEPLGLTDTLWHGFEPDAGLMQPWTLPPRPRQSHKGSNGHVLAIGGDHGTAGAVRLCGEAALRGGAGLVSVATRADHLTALNSARPELMAHDVHGPQALEALLPRATVLAVGPGLGQGAWGHALWLTALDSHLPLVLDADGLNLLAREPRELGDGAVITPHPGEAARLLGCDTATIGRDRYAAVRELARRYRAVAVLKGAGSLVADPHGRVAVCPWGNPGMATGGMGDLLTGIIAALIAQGCGAWQAACLGVSLHARAGDVAARHGGERGLIATDLLDPLRRLLNGRES",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein requires Mg(2+) to activate a critical catalytic site.2. The desired protein should be able to perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate3. The K(+), (6S)-NADPHX, AMP binding site should be located in a region of the protein that is accessible to the ligand.4. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.5. The designed protein have YjeF N-terminal domains with specific sequence motifs that are crucial for its activity.6. The designed protein should have NADPHX epimerase activity, NADHX epimerase activity, metal ion binding, ATP binding, ADP-dependent NAD(P)H-hydrate dehydratase activity to facilitate nicotinamide nucleotide metabolic process, phosphorylation. The designed protein sequence is ",
    "response#1": "MAAADEATIASGTPGFVLMERAGRAVARATVTLAGRRYGARVAIVCGKGNNGGDGFVAARVLHREGVAVRCLLLAEESEYEGDAAAHLRAMSRAGVKPRPFETHSLSYHDVVVDAIFGTGFRGRAEGRPAKAIEAINSNGARVLAVDIPSGVAGDTGAVEGPAVRANVTVAIAAQKLGTVLHPGAGRAGRVEVVDIGIELGDAEPSDIGAMLPARSVDAHKRSTGSVLVLAGSDGMSGAALLTSRGAARMGAGYVTLVSTSYVDHAKKSLIPEAVSRIVADHSELGPEVLAEFDDDFDRADAVALGPGLGTGPRQQALVETALEAIDVPLVLDADGLNVLAEDAAALRKRTSPTVLTPHPGEMATLLKLSSKDVQADRVGVARRAASDLGCVVVLKGAGTIVAGPEGPAVVNPTGGPELATAGTGDVLTGVVGALLAEGLDPVSAAIAGVFVHGLAGGVAGVSAGGRGTVAWDVAEALPAAVGELYERDDAAAALPATTPVDHKGRRGHALVIAGSPGKWGAGRMAARAALRAGAGLVTLAGPRADAAAPDEVMTAAIDPEAAGFDVALAELLAGKRAVAIGPGMPATAAGAALVRAVLERAEVPVVIDADALNHMAGDLAAVAAARAPVVLTPHPGEAGRLLGCSAADIERDRIAAVRRLAERTRAVVVLKGARTLVCDGAVDGFTTVNPTGNAAMATAGAGDVLTGVIVALAAQGAPPADAARAGVFLHGLAGDRAARGRIDAGLMAGDLIDELPRARADLTAAL",
    "response#2": "MKRLDQAAIKEYGVPGLVLMENAGRQVVEVIGDVLGIVRGKVVTVFIGKGNNGGDGLVIARHLLNMGAEVKVLALAGVEEITGDAAVNLEIWRKMGQKIYSLHHGDGINIVRLILMNSELIVDAIYGTGFRGKMEEKVGRIVEVLNGSGKPIIAVDIPSGLEANTGRVNEPCIQAAHTVTFALPKLGLILEPGADYAGELHVADISIPSVLLDRRWVAEHLPRRPLDSHKGTFGHVLVVAGSRNYVGAAYLASQASVRAGAGLTTLATPESVYPVAAAKATEPIHMPLPEDEEGRVGAQAAESLRRGSRRYTNILVGCGLGLSEGTAKFVEDLLFGGEGSGLSGLPALVDADGLNNLARIAGWPERGRCHMVLTPHPGEMATLMGLQASEIQKHRVLVAREYAAKWNVCLVLKGANTVIGEPGGMVRVAPFANPGMATGGTGDVLSGVIAGLMAQGLAPAVAACCGVYLHGEAGRETASRLGNMGVAASDLLPEIPMAVRRLKEPADYADVVAPHTNNVDKYSRGSVLVVAGSSRYPGAAVMAAKAAARAGAGYVTLAVPVTIAPLIQMQLPEVPVVGVAAEQDGTFSAAARPVILKLAERSSATLVGPGMRVTGGTVAVVSALINSKAPLVVDADGLNCLARLTNGHLDEFPEVTRRDAPLVLTPHRRELGRLVGLADTPPDSLTAALEAARRIVWADGGSELCIVAKGTATACVGVEAALLPEPGTASLATAGSGDVLGGIICALLARMPDDIEDLPLIAAFGCEVHAVAGSRAAKRYGSRGVMAGDIADEVGLAVDE",
    "response#3": "MAAADEATIASGTPGFVLMERAGRAVARATVTLAGRRYGARVAIVCGKGNNGGDGFVAARVLHREGVAVRCLLLAEESEYEGDAAAHLRAMSRAGVKPRPFETHSLSYHDVVVDAIFGTGFRGRAEGRPAKAIEAINSNGARVLAVDIPSGVAGDTGAVEGPAVRANVTVAIAAQKLGTVLHPGAGRAGRVEVVDIGIELGDAEPSDIGAMLPARSVDAHKRSTGSVLVLAGSDGMSGAALLTSRGAARMGAGYVTLVSTSYVDHAKKSLIPEAVSRIVADHSELGPEVLAEFDDDFDRADAVALGPGLGTGPRQQALVETALEAIDVPLVLDADGLNVLAEDAAALRKRTSPTVLTPHPGEMATLLKLSSKDVQADRVGVARRAASDLGCVVVLKGAGTIVAGPEGPAVVNPTGGPELATAGTGDVLTGVVGALLAEGLDPVSAAIAGVFVHGLAGGVAGVSAGGRGTVAWDVAEALPAAVGELYERDDAAAALPATTPVDHKGRRGHALVIAGSPGKWGAGRMAARAALRAGAGLVTLAGPRADAAAPDEVMTAAIDPEAAGFDVALAELLAGKRAVAIGPGMPATAAGAALVRAVLERAEVPVVIDADALNHMAGDLAAVAAARAPVVLTPHPGEAGRLLGCSAADIERDRIAAVRRLAERTRAVVVLKGARTLVCDGAVDGFTTVNPTGNAAMATAGAGDVLTGVIVALAAQGAPPADAARAGVFLHGLAGDRAARGRIDAGLMAGDLIDELPRARADWMPQRDRHAHKYSVGLALVLGGSPGLTGAPVMASLAAARIGAGFVTCACASSIQPVLALKMTAITTLALPETADGGLDPDAALAALAPRLEKARALLVGPGLGRHPATQTFIRRLLTRTDLPTVIDADGLNALAGHLDDLAEQAAGRWILTPHAGEFRRLAGTDADLTDRIRTVQTCARRWNSVLLLKGLPGLVGTPDGRVYLNPTGNPAVATAGTGDVLAGLCVGLLAQGLPPARAAVAALYAGGAAADAYTTHRHPHTMTPLDLLEELPRV"
  },
  {
    "reference": "MIPRFDFPWPSACHPHARQAEQGALAFAERHGLVPTAAYRSRLERTRYGWLAARCYPDADDVLLQLCADYFIWFFIVDDLFVDRVDTLSERTIPNLTAMIDVLDHHRPGAEPVFGEHAWLDVCTRLRAYLSDEHFQRFAHGMRMWAATAGLQIANHLGADTVDVAPYETIRRHTSGTNPCLALADAAKHGPVTPAEYHSPPVQRLVLHANNVVCWSNDVQSLKMELNQPGQYWNMAAIYAHRGLSLQQAVDLVALRVRGEIASFQSLALTLEPHASRPLRGFVDGLRHWMRGYQDWVENDTLRYADAFIAEDADDTAVRT",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: The preferred substrate is (2E,6E)-farnesyl diphosphate (FPP), however geranyl diphosphate (GPP) is also able to produce small amounts of several acyclic and cyclic monoterpenes, with linalool as the main product..2. The protein should have a high affinity for substrate.3. The designed protein must be able to perform lyase activity, metal ion binding under specific conditions. The designed protein sequence is ",
    "response#1": "ELLLKLSKVNFKFMQNLYKEELSQLSRWWNTWNLKSKLPYARDRVVEAYVWGVGYHYEPQYSYVRMGLAKGVLICGIMDDTYDNYATLNEAQLFTQVLDKWDRDEAERLPEYMKIVYRFILSIYENYERDAAKLGKSFAAPYFKETVKQLARAFNEEQKWVMERQLPSFQDYVKNSEKTSCIYTMFASIIPGLKSVTQETIDWIKSEPTLATSTAMIGRYWNDTSSQLRESKGGEMLTALDFHMKEYGLTKEEAASKFEGLVEETWKDINKEFIATTNYNVGREIAITFLNYARICEASYSKTDGDAYSDPNVAKANL",
    "response#2": "EKLLELAKLEFNIFHSLVKRELQSLTRWWKDYGFPEITFSRHRHVEYYTLAACIANDPKHSAFRLGFGKISHMITILDDIYDTFGTMEELELLTAAFKRWDPSSIECLPDYMKGVYMAVYDNINEMAREAQKIQGWDTVSYARKSWEAFIGAYIQEAKWISSGYLPTFDEYLENGKVSFGSRITTLEPMLTLGFPLPPRILQEIDFPSKFNDLTCAILRLKGDTQCYKADRARGEEASAVSCYMKDHPGITEEDAVNQVNAMVDNLTKELNWELLRPDSGVPISYKKVAFDICRVFHYGYKYRDGFSVASVEIKNLVTRTVVETV",
    "response#3": "PTLVELAKLDFDMVQAIHLEDLKHASRWWRDTSWDTKLTFARDLIVENFLWTIGFSYLPNFSRGRRTITKVAVMITTLDDVYDVFGTLGELEQFTDVINRWDIKAIEQLPDYMKICFLGLYKSINDITHETLANKGFLILPYLKKAWADLCKAYLVEAQWYHRGHIPTLNEYLDNACVSISGPVALMHVHFLTSVSSIEEIHQCIQRTENIVHYVSLIFRLADDLGTSLGEMERGDTLKSIQLHMHETGATEPEARSYIKLLINKTWKKLNKERATVNSESSQEFIDYATNLVRMAQFMYGEGDEDFGLDVIKSHVLSLLFTPIL"
  },
  {
    "reference": "MAPAPVTLLAPGAASSMSCSQPGQRSPSNDFQVLRGTELQHLLHAVVPGPWQEDVADAEECAGRCGPLMDCWAFHYNVSSHGCQLLPWTQHSPHSRLWHSGRCDLFQEKGEWGYMPTLRNGLEENFCRNPDGDPGGPWCHTTDPAVRFQSCSIKSCRVAACVWCNGEEYRGAVDRTESGRECQRWDLQHPHQHPFEPGKFLDQGLDDNYCRNPDGSERPWCYTTDPQIEREFCDLPRCGSEAQPRQEATSVSCFRGKGEGYRGTANTTTAAYLASVGTRKSHISTDLRQKNTRASEVGGGAGVGTCCCGDLRENFCWNLDGSEAPWCFTLRPGTRVGFCYQIRRCTDDVRPQDCYHGAGEQYRGTVSKTRKGVQCQRWSAETPHKLQALTLGRHALMSGTRAWKWLRLPCHDFAPAPASVHIYLRTACTTGGELLPDPDGDSHGPWCYTMDPRTPFDYCALRRCDQVQFEKCGKRVDRLDQRRSKLRVAGGHPGNSPWTVSLRNRQGQHFCAGSLVKEQWILTARQCFSSCHMPLTGYEVWLGTLFQNPQHGEPGLQRVPVAKMLCGPSGSQLVLLKLERSVTLNQRVALICLPPEWYVVPPGTKCEIAGWGETKGTGNDTVLNVALLNVISNQECNIKHRGHVRESEMCTEGLLAPVGACEGDYGGPLACFTHNCWVLKGIRIPNRVCTRSRWPAVFTRVSVFVDWIHKVMRLG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be designed to localize specifically to extracellular space in order to perform its role in regulation of receptor signaling pathway via JAK-STAT more effectively.2. The designed protein have Kringle, Peptidase S1, PAN domains with specific sequence motifs that are crucial for its activity.3. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "IVEGSDAEMGLAPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKVYIHPRYNWRENLDRDIALLKLKKPVTFSDYIHPVCLPDKETVARLFQAGYKGRVTGWGNRKEIWQSSIDEVLPKVLQVVNLPIVERPVCKASTRIRITDNMFCAGYKPEERKRGDACEGDSGGPFVMKSPFNDRWYQIGIVSWGEGCDRDGKYGFYTHVFRLKKWIAG",
    "response#2": "IVEGSDAEMGLAPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKVYIHPRYNWRENLDRDIALLKLKKPVTFSDYIHPVCLPDKETVARLFQAGYKGRVTGWGNRKEIWQSSIDEVLPKVLQVVNLPIVERPVCKASTRIRITDNMFCAGYKPEERKRGDACEGDSGGPFVMKSPFNDRWYQIGIVSWGEGCDRDGKYGFYTHVFRLKKWIAG",
    "response#3": "IVEGSDAEMGLAPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKVYIHPRYNWRENLDRDIALLKLKKPVTFSDYIHPVCLPDKETVARLFQAGYKGRVTGWGNRKEIWQSSIDEVLPKVLQVVNLPIVERPVCKASTRIRITDNMFCAGYKPEERKRGDACEGDSGGPFVMKSPFNDRWYQIGIVSWGEGCDRDGKYGFYTHVFRLKKWISE"
  },
  {
    "reference": "MTYHLRVADLPSSDRPREKLLAQGARYLTSAELIAILLGTGQGAGKLSAIGLGQYILQQLGQHQRDPLAVLREITPQELMAIPGIGPAKATTILAAIELGKRVFQSKPTEQTIINDPAVAAAALGSELFWQAQEKFAVLLLDVRHRLIGTQVITIGTATETIAHPRDIFREVIRQGATRVIVAHNHPSGNVEPSPEDLALTQQLLSGAKFLDIPLLDHLILGNGNFRSLRQTTKLWEECPQGD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must have high specificity towards metallopeptidase activity.2. The designed protein should contain at least one JAMM motif motif to achieve its intended function.3. The designed protein must have at least one MPN domain. The designed protein sequence is ",
    "response#1": "LTSPQAVHDYLRLAYGMRVQEVVLLLCLDARNRLLAVEEVAHGTLTESRVYPREVVKAALRQHAVAVILAHNHPSGDSGPSLADRELTEALGRALALVDVRLLDHLVISRQGCTSFAECQ",
    "response#2": "PVLSSAQVAEKMMIELGDKQQEHLVAIYLDSQNKIIEEKTIFIGTVRKSIAEPREILYYACKNMATSLIVVHNHPSGLTKPSANDYHFTEKIKRSCDYLGLICLDHIIVSKHGYYSFREKSDV",
    "response#3": "MGIRSWPAAERPRERLLELGAASLSDAELLAIFLRTGVAGKSAVDLARHLLNQFDGLRSLLDADQPAFTAQLGLGPAKFL"
  },
  {
    "reference": "MAEDNNQAAQGGNDAQNQLQFSMQRIYVKDISFESPNAPSVFQNAFKPKVGLDLNTTHKQVGENLYEVVVKVTAQVTNNEDGATAFLAEVEQAGLFRIAGLSDDQLDHTLGAFCPNMLFPYARECIDNLVNRGSFPPLMLSPVNFEGMYAQKKKRESEAAENAH",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state.2. The designed protein should be involved in protein transport, protein folding by localizing to cytoplasm and exhibiting unfolded protein binding. The designed protein sequence is ",
    "response#1": "QKLLDMVINSIYSQREVFLRELISNASDAIDKIYYKALTDDSLSFDVDNYYIKITPNKEERTLTVVDTGIGMTKEELENNLGVIAKSGSHTFKSENEIKDGHDIIGQFGVGFYAAFMVADKVEVVTKSIDSDAAFKWESKGSDGYSITEADKQDIGTTITLYIKENQEEENYDEFLDTFTLQQIIKKYSDFR",
    "response#2": "MLEQKLLDMVINSIYSQREVFLRELISNASDAIDKIYYKALTDDSLSFDVDNYYIKITPNKEERTLTVVDTGIGMTKEELENNLGVIAKSGSHTFKSENEIKDGHDIIGQFGVGFYAAFMVADKVEVVTKSIDSDAAFKWESKGSDGYSITEADKQDIGTTITLYIKENQEEENYDEFLDTFTLQQIIKKYSDFH",
    "response#3": "QKLLDMVINSIYSQREVFLRELISNASDAIDKIYYKALTDDSLSFDVDNYYIKITPNKEERTLTVVDTGIGMTKEELENNLGVIAKSGSHTFKSENEIKDGHDIIGQFGVGFYAAFMVADKVEVVTKSIDSDAAFKWESKGSDGYSITEADKQDIGTTITLYIKENQEEENYDEFLDTFTLQQIIKKYSDFF"
  },
  {
    "reference": "MSDNAAFKFGSFDYICEHAALVVCPMLGDQQGMAPTCYSRNVQLGSQIIFQPATCILHIAALVMATIMLLHVRSKYTAVGRKEIVLFFYMYIWVELFAIFLDSAIIPTANKVYPWFAAIYAGSVGALYWCLLLNGFVGFQFHEDGTPMSLWFLRISSLVVGAVCFGIPVATFKGTSSSMSPTNTVGLFITYLVFPCVCVLIYFISQMLLVVRTLDDRWVIGDLLFMAGFYIAGVLLLVTFSVTICDAVKHYVDGVFFSTLAFLFAVMMVYKYWDSITKEDLEFSVGSKQAVWDVKDPLLATGMEYYEDDAQSAYRGAGGSLVGGYNGNQYYGNQPGYAQSAYGQQGYGQYGAGGGYGQGHY",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. The designed protein must be able to regulate protein folding. The designed protein sequence is ",
    "response#1": "GNISIITLIRISSQLHTPMYLFLSHLAFVDILYSTSVSVIMHMELLGHGLALPVAACAAQLCITVSFGSAECFLLAAMAYDRYVAICSPLLYSTLMSPRVCFLLLGMSYVGGCMNGWTFTGCLLSLSFCGPNQIDHFFCDFSPLLKLSCSDVSIIGIIPSISSGSIIVVTVFVIAVSYIYILITILNMRSTEGRHKAFSTCTSHLTAVTLYYGTITFIYVMPKSNYSTEQNKVLSVFYTVVIPMLNPLISAVEY",
    "response#2": "GNISIITLIRISSQLHTPMYLFLSHLAFVDILYSTSVSVIMHMELLGHGLALPVAACAAQLCITVSFGSAECFLLAAMAYDRYVAICSPLLYSTLMSPRVCFLLLGMSYVGGCMNGWTFTGCLLSLSFCGPNQIDHFFCDFSPLLKLSCSDVSIIGIIPSISSGSIIVVTVFVIAVSYIYILITILNMRSTEGRHKAFSTCTSHLTAVTLYYGTITFIYVMPKSNYSTEQNKVLSVFYTVVIPMLNPLISAAMS",
    "response#3": "GNISIITLIRISSQLHTPMYLFLSHLAFVDILYSTSVSVIMHMELLGHGLALPVAACAAQLCITVSFGSAECFLLAAMAYDRYVAICSPLLYSTLMSPRVCFLLLGMSYVGGCMNGWTFTGCLLSLSFCGPNQIDHFFCDFSPLLKLSCSDVSIIGIIPSISSGSIIVVTVFVIAVSYIYILITILNMRSTEGRHKAFSTCTSHLTAVTLYYGTITFIYVMPKSNYSTEQNKVLSVFYTVVIPMLNPLISAAQSL"
  },
  {
    "reference": "MEDGVQAMRDYLAGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTPVAFLEISNKPYLGNMLNDFAGVDQQRVMHCRKAFSDAKVFK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must be optimized for transferase activity. The designed protein sequence is ",
    "response#1": "PDTVVGIAPSGAPLATLIAAEEGLKLAVYLPAKHSRSEAPTGSLSGTFSAIAAQRCIIVDDVVTTGTTLSETIAFLRQHGATPVAVWSLFDKRGAREIDGVPVHSLFE",
    "response#2": "WDSVRASSKRLKHIAKIMCDILGQVEFDGIVGISSGGVPLATLISDEMDKNFSIFVPKKHIHTEKEKTTGFIGQNFSSIVGKDVIIVDDVMTSGNAVKEAIKYLKSISNPKMVVVVMDR",
    "response#3": "EFDGIVGISSGGVPLATLISDEMDKNFSIFVPKKHIHTEKEKTTGFIGQNFSSIVGKDVIIVDDVMTSGNAVKEAIKYLKSISNPKMVVVVMDKSGIDEIDGVPVHHLIT"
  },
  {
    "reference": "MNLRPSHPAGPALGPLAEQVQAVPAAGGAVPQINVTGVTLRSADVEPGDLFAALPGTSSHGGRHVGEAVARGAAAVLTDPDGLALLGAEVDVPVLLHRSPRSVLGELAAEVYGRPSERLRVIGVTGTSGKTTTTYLVEAGLRSAERVAGLIGTVGVRVDGRDQASALTTPEAPDLQALLAVMVEQGVDTVVMEVSSHALSLGRVDGVRFAVAGFTNLSRDHLDFHPTMRDYFEAKARLFDPESRNCADVSVLCVDDDAGREMARLARDPVTVSATGRDADWRVEDIVTVDRNAQEFFAVDPAGVHHGLRIALPGRYNVANCLLAAALLDAVGVSPEQAAPGLRNATVPGRLEAVERGQDFLALVDYAHKPGALQAVLETLRAQTQGRLAVVFGAGGNRDQGKREPMGRVAAELADLVVVTDDNPRDEDPATIRAAIVAGASCGSTQVVEIGDRRAAIAHAVAWARPGDVVLIAGKGHEAGQTTAGVTRPFDDREALADALEAAGHRP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that can perform UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity in cytoplasm to support cell wall organization, regulation of cell shape, peptidoglycan biosynthetic process.2. The UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP binding site should be located in a region of the protein that is accessible to the ligand.3. The protein should have a fast turnover rate for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.4. The protein should be optimized for activity in Cell wall biogenesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. The designed protein must have at least one Mur ligase C-terminal domain. The designed protein sequence is ",
    "response#1": "MSVTGTNGKTSCAHWIAAGLDASGRRAAVLGTLGNGLWGALEDAPNTTADAALLHETLRHLASIGAQAAAMEVSSHGLDQGRVNGVAFDVALFTNLSRDHLDYHGTMAAYGAAKAKLFAWPGLRFGVINADDPFGQSLIDAARSRGRKVLTYGFGAADIVGTHLAASSTGLAFAVETPWGKGEIHTRLVGAFNAANLLGVLGTLAGLPLAALQAVTGTSGKTSVAAFTREIWSALGEPAASIGTVGVVSPKGEVYGSLTTPDPVDLHRSLDELAGDGVTHLALEASSHGLDQYRLDGIRIAAGAYTNLSRDHLDYHPTLEAYLAAKLRLFTDLIAANGTAVINVDDEHAPQVVEAARQRGLKLMTIGRRGEGIRIADVAIDGFAQRVEFAHAGHIYNVKLPLVGAFQVYNAAVAAGLAIACGAEPQAVFHALAQLTGVRGRLELAGSYNGAPIFIDYSHKPDALAKALEALRPYASGHLVVVFGAGGDRDPGKRPLMGEVAQQKADRVIVTDDNPRSEEPAEIRAAILAAAPGATEIGDRGQAIRAGIAGLRPGDVLLVAGKGHETGQIVGSRVLPFDDAEQASVAVAALEGRL",
    "response#2": "MQIARITSDSRQVRPGDAFAAYRGTRQDGRAFIPDAIARGAGAILWDVQGFVWNREWKVPHLPIEDLKARLGAIADAFFGFPSKALDLVGIGVTGTNGKTTVTHLIETIMQDAKKKAGVIGTIDYRIDGKKVESKLTTPEAPDLQRMMAEMVDAGCRAAVMEVSSHALALDRVAGCRFEVAVFTNLTQDHLDYHGTMENYFAAKGRLFTGLGKRSGSKPRVAVVNRDDPWGQRLVEETPAAVLTYGIDQPADLRATRVEVSAGGTRFVLETARGEAELRSSLIGRHNIYNILAACGAGLALGIPLEDIAKGLEEAKGVP",
    "response#3": "MKLRDLVNGIEFEIIHGSADVQVVEISHIAQDSRKVKPGTLFICIDGELVDGHQFASRAVELGAVAIIAEKQLDVSIPVIYVRDSKRAMAMLASRFYPRQPDTLVGIGVTGTNGKTTVTHLIETIMQDAKKKVGLIGTIANYIGTTTLPSPRTTPDSLDLQRLFSEMRDAGVTDVAMEVSSHALDMGRVRGCDFDVAVFTNLTQDHLDYHKTMTEYMSAKLKLFSGLGKHGDKKAVINIDDEYGARIVKATKAKVITYSLNRAADLSAKDIHQTSSGIDFTLHTPWGSGEVESKLLGSFNISNILGVIGVMVALGFSIDEAVRVISTLEGVPGRMQRLGGQGAPLVVIDYAHTPDALDKVLTSLRPAVAVGGELVCVFGCGGDRDRGKRSEMGRVAARLADRVVVTSDNPRSEEPGAITSDIVHGIRETGNRRYAVEIDRAAAIATAIAEARVGDVVLLAGKGHEDYQILGTEKIHFDDREVAAAALRERQ"
  },
  {
    "reference": "MSGYRVGILGATGAVGREMMKVLEEYRFPVKELIPLASQRSAGKKLPFCGEEVTVREATPSAFEGMDIVLGAAENEIAKAFSSDIVKAGAVFVDNSSAFRMDPEVPLVVPEINGEDAKAHKGIIANPNCSTIITLMAVAPIARISPIRKMIASTYQAVSGAGVGGLRDLENQVKAYAKGEELVVDTFTYPIAFNLIPCIGSEKERGYTSEEMKMQNEGRKILHLPELRATCTCVRVPVLRSHSISVTVVTEQKVTVEEARRAISSFPGDILADDLSGKLYPMPLHSSDKDEVLVGRIREDLTEENGLSLFCCGDQLRKGAASNAVQIAQLLIR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a high specificity for phosphate binding, with minimal off-target effects.2. We require a protein that can specifically bind to the metabolites produced in the L-threonine from L-aspartate: step 2/5, L-threonine biosynthesis, Amino-acid biosynthesis pathway.3. The protein with the reaction L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH, the Acyl-thioester intermediate, Proton acceptor must be optimized to ensure efficient catalysis.4. A protein with protein dimerization activity, NADP binding, aspartate-semialdehyde dehydrogenase activity, NAD binding functions is required to enable lysine biosynthetic process via diaminopimelate, diaminopimelate biosynthetic process, threonine biosynthetic process. The designed protein sequence is ",
    "response#1": "MRVVIVGATGNVGREMLSILHERQFQVDEIAVLASRRSLGTQVSFGDKTLVTKDLETFDFSGWDIALFAIGSDGTKKHAPRAASTGCVVIDNSSLYRYDPQVPLIVPEVNSDKIYDLKPIHDAVGISRINVATYQAVSGAGKEMIEDLVNQTSSLLNGKPVSTKGKTKQIAFNAIPHIDNFQENKYTKEEMKMVWETQKILEDSSIMVNPTAVRIPVFYGHSEAVHMETKSKISCNEVIDLMNQTDGVEVIDGVELGAYPTAVSDASGADPVYVGRIREDISHPNGINLWIVADNIRDGIIIVNCTCVRVPVVRSHLKPIHDAVGISRINVATYQSVSGTGKKAISELISQVGELLNGRPVKASVYPQQIAFNALPHIDEFLENGYSKEEMKMVWETKKIMEDDTIRINPTTVRVPVLYGHSEAVNVELKAPLSASDARNILRSAPGIKVVDDLANLEYPTVITHAIGHDEVFVGRIREDISFPCGLNMWIVADNIRGAHGAMF",
    "response#2": "TMAIVGATGAVGQEMMKVLAERDFKIDELICLADPREEGTQLKFKDEVITVQKVTEEAFRKADIALFAVGTEISKELAPIAVKNNCVVIDNSYAFRLEEGVPLVVPEVNAEDIKSLKPIHDAVGISRINVATYQAVSGAGKEMIEDLVNQTSSLLNGKPVSTKGKTKQIAFNAIPHIDNFQENKYTKEEMKMVWETQKILEDSSIMVNPTAVRIPVFYGHSEAVHMETKSKISCNEVIDLMNQTDGVEVIDGVELGAYPTAVSDASGADPVYVGRIREDISHPNGINLWIVADNIRLKPIHDAVGISRINVATYQAVSGAGKEMIEDLVNQTSSLLNGKPVSTKGKTKQIAFNAIPHIDNFQENKYTKEEMKMVWETQKILEDSSIMVNPTAVRIPVFYGHSEAVHMETKSKISCNEVIDLMNQTDGVEVIDGVELGAYPTAVSDASGADPVYVGRIREDISHPNGINLWIVADNIRNPIHKEYGLKRVVVSTYQAVSGAGKAGIDELDEHIKAYMKGEEMKPSVFQYPIAFNLIPHIDVFVEHDYTREEMKMVWETRKIMHLLDLKIAATAVRVPVYRSHSESINLETYKPVTPEGVREVLSRAPGVIVQDNPQEKLYPMPLYTSHKDEVFVGRIRKDISTENGIAMWVVADQIRISPIRSMIASTYQAVSGAGAGGPAELMQEVEALARGEAAEPQVFPYQIAYNVIPQIGGESTDGYTSEEMKMQNEGRKIMHLPGLLVNCTCVRVPVVRSHSISVTVQTERPVSVEEARAAIAKAPGCRLADDLAGRIYPMPLETSDQDLVFVGRIRRDLTHENGLAIWCCGDQVR",
    "response#3": "LGGLFRENLVEWATSMTYQAASGAGAQNMRELLQQMGEAHRVAGDLLRDPASAILDIDREVAGTLRDERFPTAHFGVPLAGSLIPWIDKDLGNGQSKEEWKGGAETNKILGREANPTPIDGLCVRIGAMRCHSQALTIKLTRDVPLDEIDAIIASANSWVKVVPNEREASMHELTPTAVTGTLAVPVGRLRKMNMGSEYLSAFTVGDQLLARPVPLYYVNPIHKEYGLKRVVVSTYQAVSGAGKAGIDELDEHIKAYMKGEEMKPSVFQYPIAFNLIPHIDVFVEHDYTREEMKMVWETRKIMHLLDLKIAATAVRVPVYRSHSESINLETYKPVTPEGVREVLSRAPGVIVQDNPQEKLYPMPLYTSHKDEVFVGRIRKDISTENGIAMWVVADQIRNPIHKEYGLKRVVVSTYQAVSGAGKAGIDELDEHIKAYMKGEEMKPSVFQYPIAFNLIPHIDVFVEHDYTREEMKMVWETRKIMHLLDLKIAATAVRVPVYRSHSESINLETYKPVTPEGVREVLSRAPGVIVQDNPQEKLYPMPLYTSHKDEVFVGRIRKDISTENGIAMWVVADQIRREGVESAISPIRSMIASTYQAVSGAGAGGPAELMQEVEALARGEAAEPQVFPYQIAYNVIPQIGGESTDGYTSEEMKMQNEGRKIMHLPGLLVNCTCVRVPVVRSHSISVTVQTERPVSVEEARAAIAKAPGCRLADDLAGRIYPMPLETSDQDLVFVGRIRRDLTHENGLAIWCCGDQVR"
  },
  {
    "reference": "MSVTAAEGFVAAGHAAGIKTGGRRDTSLLATDDGKPVPTAAVFTQNKFRAPPVDASLERLNVSGGMASGVMVNSGNANAGTGAKGREDAETMCAEAAKAVGCDPRDMLVCSTGLIGFRLPMRRILAAIPALGETLSRDGHRDAAKGILTTDTRPKEAAIARDGYTVGGMAKGCGMLAPNMATMLAFMTTDAELSPSELKPILQRATDATFNELITDGATSTNDTVMLFASGRKGRPADLAAFEDDVRQVCEELMLMMARDAEGMTKLVIVRVVGARSDAEARLVARSIGNNNLIKCSWYGADPYWGRLLAEAGSCGAEFDTLTSAVSYGGVKVAQAGVEIPHDADAVRAHVEGAEIDILIDLGLGSGKGRAVSVDLGPGYIKENAATS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The substrate binding site should be located in a region of the protein that is accessible to the ligand.2. The designed protein should be involved in arginine biosynthetic process by localizing to cytoplasm and exhibiting glutamate N-acetyltransferase activity, methione N-acyltransferase activity, acetyl-CoA:L-glutamate N-acetyltransferase activity.3. A protein should be able to manipulate the activity of enzymes in the Amino-acid biosynthesis, N(2)-acetyl- L-ornithine from L-glutamate: step 1/4 pathway, either by activating or inhibiting their function.4. The Nucleophile of the protein must be engineered to enable effective catalysis of acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate.5. For general function, the protein need meet that Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate. The designed protein sequence is ",
    "response#1": "MTAATPTSVPGTTPGSAPAAAAAAPPVRVAGPAGRAALGELAALLGLPAPRPPADLAWLGGEDSQDLAXAAXVXGRGLALDAVLADRAAAFGVPVVAVPGAGGVRXGPVXVXGTDPRHALAELAGXFYADPSXALXVVGVXGKSTTVNLLAHALXVAGRPAALIGTLGYGFPGQLQPAARTTPDPVTLQSLLADMVDAGVTAVAMEVSSHALAQGRVDGVHFDVAVFTNLTRDHLDFHGTMEAYAAAKARLFADLDAVAAAXADAGAAXPPXGYAVVNADDEAGAAXLXAAXAXVVTVGLDXPAXVRATDVTVTRDGSRFDVVLDGREXRLXLRLXGRFNVSNALAAVGAALALDVPXEAVVAGLEAFPGVA",
    "response#2": "MTTDSRTGTPRSSPDNTAGIRLGGLDLPADGEPEPVLPPVPGTGGAPVRRSGLLSRLGLGRRGPAPVPPAAVAPAPAPAPAPEPAEAAAPVAEARPAPAEPAAEPVAAPVPLVAPAPVAPAPVADAAPPVAAAVASAPVAEAPVAEAPVADAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAAAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAPVAEAP",
    "response#3": "MIYTANPAXXNXPXSSLHXAXGXSXXDXGXPAPAXNPXQXHXSXSXDXNXDXI"
  },
  {
    "reference": "MKKIVLASRNPGKLADFSILLASLQIELASVADFTDEEVAETGGTFIENALLKARHASGVSGLPALADDSGLVVDALGGAPGIYSARYAGDGVDDSDNNQKLLAEMAGFDGDRRAAHFHCSLVLLMSADDPAPLIGEGRWGGMIAHQASGVAGFGYDPLFVPVGETRAVAEFSAVEKNRRSHRGLAWQRLRPLLNG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.2. The protein should be able to modulate purine nucleoside triphosphate catabolic process in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "GLLSKIIGK",
    "response#2": "MGHRKLFNP",
    "response#3": "MGQ"
  },
  {
    "reference": "MRVVVQRVRAASVAVDGDIVGKIGTGLLVLAGFEEADSETDLDWMAGKIVRLRLFADESGVMNRNVLDAGGEVLAVSQFTLYASVKKGNRPSWSRAARGEVSQPLFERFVTKLSATLGKAVPTGVFGADMQVSLINDGPVTLTIDSKVPE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS; An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs; Acts via tRNA- based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site; By recycling D- aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl- tRNA entities in vivo and helps enforce protein L-homochirality..2. A protein that contributes to D-amino acid catabolic process by functioning in cytoplasm with D-aminoacyl-tRNA deacylase activity, Gly-tRNA(Ala) hydrolase activity, Ser(Gly)-tRNA(Ala) hydrolase activity, tRNA binding.3. The protein design must feature the Gly-cisPro motif, important for rejection of L-amino acids motif.4. We are looking for a protein with broad substrate specificity for a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + a tRNA +   H(+). The designed protein sequence is ",
    "response#1": "MNDPQQTIPTTHRPAHPSTAPPHRTGRPGRTAGLADALAAAGLTPRARRCLGPDDAALLEQAQHVLGLPVCYVQRSSGAVWPVVADGTRPDGTTVTMSCSCPLGEHCRHAVAVLLLAADRLTAAAGTLGEGHDPAGDTSAEAVAPWLEAQTVRELRSRLLDASRDEVGQLLAGAASAASAGGGTRPGGRPGARAAAAAAGTVRPRAGLGLTGGRGARLTVTAVRLGA",
    "response#2": "MASGSTLARGFRLSLLGLLAFGALVVRLWRLDFPPGLHADEAQNGLNALEALEGERLTFFSANNGREGLYLTSLAAWFKLFGANALGFRLLSALAGTASVLCIYLCGRELFGRRAAFVGAALMALSFWHLQFSRIGFRAIMLPLLMTLSFWLFLRGCRRSGWRDGVAAGVALGLNFYTYMSARLVPLFLLVFVLLFLWQRDLLRPNRRALWLGMAAVFLIAVLPLTRYYAANPADTLSRFGEVSIFSANNPAIDTGRTLPVNIFFHNLFMKGLLYFDHDPRHNHNLPGRPAFDPLTGLVFLVGGLAMLWQRWRERWLLFAWFAMAPFPSALTKGAEAPHALRSMVIMPFVLIMVAVGLQWAAHWLAERGAGRVRAAMVALLALVLADYAWVYFVRYPLTYAETFYESYRQMVLARPEYDKIVFDSYYGGAPAITTGLLYYDGPQRVRELAALKPGQSFADGDSLVLPAGARLLVFPEDRLPTDLAAKLPAGATVIRRFPAILFNDGGLALISYRR",
    "response#3": "MIASAXAXTXKXDXSXIXGXPXGMAXAXTXGXXAXPXXT"
  },
  {
    "reference": "MADPQPTGEPKEPDAQSAGRRWPSIWMVVVTVVAINWLIAAVVSSSDRTPSVPYSVFRQQAEQGNVLSVRSQAETIEGRFRRAVQVVDGDRKRETTTFETERPAFADDALLGLLLGKKVKIDARPPAKASPLLSILIGVVPTLLILGVLFYAMRHAGGGGGLFGTAGRSKARRYEATGTGTSFRDVAGIDEATDELAEVVDFLRHPERYRRLGATVPRGVLLTGPPGTGKTLLARAVAGEAEVPFFSLSASEFVEMFVGVGASRVRDLFAQAKSSAPSIVFIDELDAIGRSRGSGGPTGSNEEREQTLNQILTEMDGFTGTEGVIILAATNRPEILDPALLRAGRFDRRIAVNPPDRVGREKILRVHTRSVPLDERVDLATIAAQTPGMVGADLRTLVNEAALGAARAERAAVAPEDFADALERLMLGAARRIVLSYGDRRRTAYHESGHALLGVLQPGADPVRKVSIIPRGHALGVTFQSPSDDRYGYGEPYLRGRITGMLGGRASEQLVLGEITTGAESDLEQATALARQMVGRWGMSEKLGLLTALPNREGGVIGFAGLPAGTSERTLELIDVEARRILDDCYRDAVRTLREHRDELDALAAALLVHETLDEEAIYATVGRTPVAPASSPNPASTPTPTPAPTPTPGPEPAVGA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must bind to Zn(2+) in order to perform its enzymatic function.2. The designed protein have AAA+ ATPase domains with specific sequence motifs that are crucial for its activity.3. The protein should have Helical domains to ensure proper insertion into the membrane.4. The protein design must be able to perform ATP binding, metalloendopeptidase activity in order to contribute to protein catabolic process.5. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins The designed protein sequence is ",
    "response#1": "VLLVGPPGTGKTLLAKAIAGEAGVPFFSMSGSEFVEMFVGVGASRVRDLFRKAKESAPCIVFIDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNSGVIIIAATNRPDVLDSALMRPGRFDRQITVHLTPLADTVEMIRGLKARGLKAADVNPAVIARGTPGFSGADLANLVNEAALFAARSNRRVVRMEEFDAETGRFG",
    "response#2": "APKGVLLIGSPGTGKTLLARAVAGEAGVPFFSMSAAEFVEMIVGVGAARVRDLFKQAREHAPAIIFIDELDSIGRARGQTVIGGSSEQEQTLNQILTEMDGFSSREGIIVLAATNQPDVLDKALLRPGRFDRRVVVNLPGLEERERLVRLQCEEHRIPFAPNVEVKTLARGTPGFSGADLANLVNEAALLAARRGRRLVTMAEFSWAVNRILLDSKKKRLIAYHEVGHALLMTLLKHSDPLNKVTIVPRSGGIGGFAQQVFNEEMVDSGLYTRAWILDRIVMTLGGRAAEQEVFGDSEITVGASNDLKVVSTLAREMVTRYGMSDLGTLALESQSGEVFLGRGWMSQPEYSEEVAAKIDRAVREIAVNCYEKACQILREHRELVDRLVDQLLDEEMIEGDEFRKIMGLG",
    "response#3": "IPKGILLVGPPGTGKTLLARAAAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFENAKKNAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFGGNEGIIMIAATNRPDILDPALLRPGRFDRQITVDRPDFGFADGTPEEMLEAVLDRWWAHD"
  },
  {
    "reference": "MFMVNLLLLIIPALIAMAFLTLTERKILGYMQFRKGPNIVGPYGMLQPIADAMKLFMKEPLLPTTSASTLYMTAPTLALSIALLMWSPLPMPHPLINFNLGLLFMLATSSLAVYSILWSGWASNSNYALIGALRAVAQTISYEVTLAIILLSTLLMSGSFNLQSLITTQEHSWLLLPSWPMAMMWFTSTLAETNRAPFDLTEGESELVSGFNIEYAAGSFALFFMAEYMNIIMMNALTTTIFLAMPYNMASSELYTMNFMTKTLLLTTLFLWIRTAYPRFRYDQLMHLLWKKFLPLTLALCMWYISMPALTSGIPPQT",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. We need a protein that can perform a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +   NAD(+) with high efficiency.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The ideal protein would be one that plays a critical role in mitochondrial respiratory chain complex I assembly, mitochondrial electron transport, NADH to ubiquinone through NADH dehydrogenase (ubiquinone) activity within respirasome, mitochondrial inner membrane.4. The protein must exhibit the following characteristics: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor; Essential for the catalytic activity and assembly of complex I.. The designed protein sequence is ",
    "response#1": "LMSVSILCLFFLINPYQIMIYLMIMTLFLLAKGLINNAESFINFFFFDLMSLSMVILTVWISILMIMASKSNNNYKNKIFNFYLLLMMNLLFL",
    "response#2": "LMSVSILCLFFLINPYQIMIYLMIMTLFLLAKGLINNAESFINFFFFDLMSLSMVILTVWISILMIMASKSNNNYKNKIFNFYLLLMMNLLFSSHWFMTWLGFEMNMMAIVPVLMKKYSPRSMEAATKYFLTQATASMILMLAIIINLMYSGQWTIANMENHTASMLITIALVMKLGLAPFHFWVPEVTQGIPLSSGLILLTWQKIAPLSLLYQIHSSINMELFLTMSLLSIVIGGWGGLNQTQLRKIMAYSSIGHMGWMMAIMNYNPNLSFLNLLVYILMTSSMFALLIFSSTTSTLSLSLAWNKTPIIATMSLIILLSLGGLPPLTGFMPKWMIIQELTKNNSTILPTLMAISALTASYTLNLFFYMRLAYSTTLTMFPTTNNTKLSWQFKNTNILPTTAPLISISTMILPLTPLLLAVS",
    "response#3": "LMSVSILCLFFLINPYQIMIYLMIMTLFLLAKGLINNAESFINFFFFDLMSLSMVILTVWISILMIMASKSNNNYKNKIFNFYLLLMMNLLFL"
  },
  {
    "reference": "MRQDPIIALDFATEAEVMSFLAQFDEPLFVKIGMELFYQTGPALIDQIKALGHDIFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHATGGTVMMQRAVEGLRRHDKAIKIIAVTQLTSTSEQQLHEEQNIQTSMETAVLNYASLAQQAGLDGVVCSPLETTMIREHCGDDFLKVTPGIRLANSAKDDQQRITTPEKARQLGSTHIVVGRPITQSDNPVQSYHQIKEXLVKCMSKLIAKALLDIEAVSLSPNEMYTWSSGIQSPIYCDNRVTLGYPEVRTAIRDGLIELIEQHFADVEIVSGTATAGIPHAAYISDKMNLPMNYVRSKSKSHGKQNQIEGARSEGKKVVVIEDLISTGGSSITAVEALQEAGAEVLGVVAIFTYGLNKAGETFKATGVPFYTLSNYDELIEVAREEDQISEDDIQTLVEWRNQL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein have Orotidine 5'-phosphate decarboxylase domains with specific sequence motifs that are crucial for its activity.2. A protein should be able to manipulate the activity of enzymes in the UMP biosynthesis via de novo pathway, Pyrimidine metabolism, UMP from orotate: step 2/2 pathway, either by activating or inhibiting their function.3. The designed protein should have orotate phosphoribosyltransferase activity, magnesium ion binding to facilitate 'de novo' pyrimidine nucleobase biosynthetic process.4. We are looking for a protein with broad substrate specificity for diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D-   ribose 1-diphosphate + orotate.5. The designed protein should have a high specificity for orotate, 5-phospho-alpha-D-ribose 1-diphosphate, substrate binding, with minimal off-target effects.6. Mg(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "MLDVVLANAKESGVMPAAVGGMAIGADPITASIVTLAGGDDVDLKGFMVRKEPKGHGMGQQVEGPVTPGQKVVIVEDVITSGGSALKAVEAVQAFGLEVQYVLAIIDRLAGGAEAFAQKGLELKTLTLLCIGLDPHPADLPAPTADAARAFCLCLIDATADLAAAYKPNAAFFEAFGPAGWAVLPEIIAAIPTDVPVILDAKRGDIASTAEAYARAAFETLGANAITLSPYMGRDSLAPFLADPARGVFLLCKTSNPGAGDLQDLLLANSDRPEMLYERVARLARAWNRQDNLGLVVSATHPEALARVRQIAPALWILAPGVGAQGGDPESALAAGLRADGLGLLLPVSRAVAHAADPRQAARELAHAARAETCDAGGIRVGDFVLHSGQRSPIYIDLRILPSHPFLLDEVAAIYARLLSRLTFERIAAIPYAALPIGTAVALRTGMPLIYPRREVKAHGTARPIEGFFNPGDRAVVLDDLVTTGASKLEAVNLLREAGLEVQDIVVLIDRSRGAAAELAQEGLRLHAAYTLESEYSAPLLLCVGLDPRPETFPPELQKAGDPIFEFNRQVIDVTEDLVCAYKLNYAFYEAQGMAGLSALHRTMDYIPSHLPMILDFKSGDIGSTAKAYARAAFQVWGADAVTINPYVGWDALEPFCTHKGRGVFVLCHTSNPSAGELQEVWSGGRPLYHRVARLAQEKWNVYGNVGLVVGATYPQAVAEARALAPDLWFLLPGVGAQGGDLEAALAAGLRADGQGVIVAVSRGVAQAVDPRAAAQR",
    "response#2": "MLDVVLANAKESGVMPAAVGGMAIGADPITASIVTLAGGDDVDLKGFMVRKEPKGHGMGQQVEGPVTPGQKVVIVEDVITSGGSALKAVEAVQAFGLEVQYVLAIIDRLAGGAEAFAQKGLELKTLTSHILFEDRKFADIGSTAMVHDAGGIRVGDFVLHSGQRSPIYIDLRILPSHPFLLDEVAAIYARLLSRLTFERIAAIPYAALPIGTAVALRTGMPLIYPRREVKAHGTARPIEGFFNPGDRAVVLDDLVTTGASKLEAVNLLREAGLEVQDIVVLIDRSRGAAAELAQEGLRLHAAYTLEEQCELLCIGLDPHPADLPAPTADAARAFCLCLIDATADLAAAYKPNAAFFEAFGPAGWAVLPEIIAAIPTDVPVILDAKRGDIASTAEAYARAAFETLGANAITLSPYMGRDSLAPFLADPARGVFLLCKTSNPGAGDLQDLLLANSDRPEMLYERVARLARAWNRQDNLGLVVSATHPEALARVRQIAPALWILAPGVGAQGGDPESALAAGLRADGLGLLLPVSRAVAHAADPRQAARESNLVLSLDIDDPDRFFAVLEATADHLVMVKTHADIMRGESTAFVERLTETARAHDLLLFEDRKFADIGSTVRKQFRGGAGRIAEWADLVTVHMIAGEAILDGLFGDSEGLHPDQGALLLARMSSQGNLITDEYTRRVIETGARRSDRVAGYIGHGEDEADLRAMRDRIPAGQLLMVPGVHRAKAGDDLGQHYLDPATAVRAGADAIIVGRGIYAAADPAAEA",
    "response#3": "MLDVVLANAKESGVMPAAVGGMAIGADPITASIVTLAGGDDVDLKGFMVRKEPKGHGMGQQVEGPVTPGQKVVIVEDVITSGGSALKAVEAVQAFGLEVQYVLAIIDRLAGGAEAFAQKGLELKTLTLLCVGLDPQPELIPSRYRTIGEFNRAIIDATAEVACAYKPNIAFYEALGEPGLQALRETLDYMPADVPVILDAKRNDISSTADAYARAAYEVWGADALTVNPYLGRDGVEPFLRYRDRGVFVLCKTSNPSAGELQDWSSGGEPLYREVARLATIWARGGDIGLVVGATYPEVVADLRTQCPGAWFLVPGVGAQGGDLEAVLRAGLRADDLGLLINASRSILYADDPGRAARLLCIGLDPHPADLPAPTADAARAFCLCLIDATADLAAAYKPNAAFFEAFGPAGWAVLPEIIAAIPTDVPVILDAKRGDIASTAEAYARAAFETLGANAITLSPYMGRDSLAPFLADPARGVFLLCKTSNPGAGDLQDLLLANSDRPEMLYERVARLARAWNRQDNLGLVVSATHPEALARVRQIAPALWILAPGVGAQGGDPESALAAGLRADGLGLLLPVSRAVAHAADPRQAARELLCIGLDPHPADLPAPTADAARAFCLCLIDATADLAAAYKPNAAFFEAFGPAGWAVLPEIIAAIPTDVPVILDAKRGDIASTAEAYARAAFETLGANAITLSPYMGRDSLAPFLADPARGVFLLCKTSNPGAGDLQDLLLANSDRPEMLYERVARLARAWNRQDNLGLVVSATHPEALARVRQIAPALWILAPGVGAQGGDPESALAAGLRADGLGLLLPVSRAVAHAADPRQAARE"
  },
  {
    "reference": "MAQHSWPELRLDDPKSFTKEYAEALDAQDPLRQLREEFVIPTRAGLKRKRLGTDSGTVEVSETINSLLPENDLTYPSTYLCGNSLGLQPRRTQEYIRKYLDTWANKGVYGHFQPLEDAVSKPWVDIDEDAKVESAKIVGAQPEEVAVMQTLTANLHLLMASFYRPTKDRYKIIIEGKAFPSDHYAVQSQIQLHNLNPSTALVELNPPDPDTSYLPTSYILSVIDAHASSTALLLLPGIQYYSGQFLDIPTITAYAQARGIVVGWDLAHAVGNVPLQLHDWNVDFAAWCNYKYMNGGPGVIGGLGWWGHDKSTRFQMRSTFVPMPGAAGFQLSNPSALDTTALLASLSVFAQTSVADLRAKSLRLTAYLEHLLFSTRTANDAEDGSSRLYSLITPRAPEERGAQLSLLLKPGLLERVMEELEHEGVVVDERRPDVVRVAPVPLYNGFADVWRFVDVFGRACKKAAAAAMEK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Ideal protein should have a binding affinity for key enzymes in the Cofactor biosynthesis, NAD(+) biosynthesis pathway to enhance the overall efficiency of the pathway.2. The protein must be engineered to have a high affinity for pyridoxal 5'-phosphate in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "LLPTAPVPKMKLDRAWDEATLHNLTQLIYDVRKDDALFRRNISTPGSFDKMRKEYWDRREYSAVELTGDESCNLTPLSKLL",
    "response#2": "HREWMDIANTVTPMLADLIGAKEREVACMGSLTANLHLMLSAFYKPTPSRYKILCESKAFPSDQYAFASQALFHGYDPKDAIVEVFPREGEYNLRVSDILDEIAKQGSAIALVIFSGVQYYTGQLFPMKVITRAAKEHGCLCGWDLAHAIGNVPMSLHEWDVDFAVWCSYKYLNSGPGGIGGLFVHEKWESTKTPQFAGWWGHDPSTRFEMPPMFSPIPGAQGFQQSNPSVFATVSLLGSLQVFKQVGMMQPLRERSVSLTARLEALLRE",
    "response#3": "HREWMDIANTVTPMLADLIGAKEREVACMGSLTANLHLMLSAFYKPTPSRYKILCESKAFPSDQYAFASQALFHGYDPKDAIVEVFPREGEYNLRVSDILDEIAKQGSAIALVIFSGVQYYTGQLFPMKVITRAAKEHGCLCGWDLAHAIGNVPMSLHEWDVDFAVWCSYKYLNSGPGGIGGLFVHEKWESTKTPQFAGWWGHDPSTRFEMPPMFSPIPGAQGFQQSNPSVFATVSLLGSLQVFKQVGMMQPLRERSVSLTARLEALLRRL"
  },
  {
    "reference": "MAERNKIELLGMNEPELEKFAVSIGEKPFRGRQIHQWIYQKEVSSFYEMNDLSKSLRQKLDDMAQISILRVLKQKVSDDGTRKFLMELNDKKKIETVVIPQSYDKNTRFTLCLSSQVGCPVGCSFCATGRSGFQRNLLASEMVGQVLGSNRELSRRLKVNGERPISSIVYMGMGEPMFNYDEVIKSIHILNDSSGMNIGQRHITISTSGEVQGIARLAHEELQVTLAVSLHACNNQLRDRLIPMNKKYPLEVLLPALRAYIDKTRRRITFEYIMLDNVNISHKDAQDMIKMLKPFLANVNLIPYNEVKDAEYSRPSSHKIKAFYNWLVEGGLTVTMREERGADIDAACGQLAARKER",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should incorporate a binding pocket that specifically recognizes [4Fe-4S] cluster to enhance enzymatic efficiency.2. The [4Fe-4S] cluster binding site should be located in a region of the protein that is accessible to the ligand.3. A protein that contributes to rRNA base methylation by functioning in cytoplasm with tRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding, tRNA binding, metal ion binding, rRNA binding.4. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs..5. The protein with the reaction adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine, the S-methylcysteine intermediate, Proton acceptor must be optimized to ensure efficient catalysis.6. The protein contains novel Radical SAM core domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "KTACVSSQIGCSLSCKFCATGYMERERNLEFDEIYDEVVLINQQSERIYDKKLSNIVFMGMGEPLLNYKNVLRAIEKISSPDGLGMSQRRITVSTAGVAKMIRQLGDDQVRFKLALSLHAANDQKRHAIMPINDTNNIRSLIDALNYFYKQTGNEITFEYILFRNFNDSTKDADELVKIYRQVPADLVNIIEYNPIDKALFEKPDDETVEKFMNAQVERSLSDERRNTLCVSTQVGCKMGCLFCMTGKQGFQANLCAGEIVNQLRSLPERNTITNIVFMGMGEPFDNLDAVMQAQEILTSEYGFALSVRKVTVSTIGLVPAMKVFLEKSKCNLAVSLHTPFDEERRKLMPIQHVYPIKEVINTINQFDLGKYRRVSMEYIVFSGLNDTSSHVKELARLLNKTRCRINLIRFHPVPGVPLPGTDEEKLLLFQRALNDKGIITTIRASRGLETDAACGQLRARRG",
    "response#2": "SSQVGCAVNCQFCLTAKLGIKRNLTAGEIAGQVAAVLNRHSVKMGKDRINLVFMGMGEPFLNYDAFMDSVRLLVEGVGIPDSRMTVSTSGIEPSIRRFATETVRPKLALSLNASNDAVRTEIMPITKKWNIAMLLDAVKTIPMGKRDWVTFEYVMLGGVNDQDFMLYPEREGLGEPSYRGGQILEWLWQKRATSIEGMSNLPVRLREHLEESFTLRSLSPAKSQGSRDSTRKFLFRLNDGRYIECVLIPASPALYGERSDRRTLCVSSQVGCAFGCKFCASGLDGFTRNLTADEIAGQVLLAEELSGEKVNNIVFMGMGEPLANLPQLLKALELITSPWGLGIGARHLTVSTSGLVPQIRKLADHPQQIRLAISLHGASNDVRDRIMPVNRKWPVEDLFKALSYWTKRKNQKITLEYILIDGINADPAQARLLAQHAESLRAKVNLIPYNTVDGLKWKRPPEEECHAFRDILAARGIPATLRLEKGHDIDAACGQLRLKEERAEKQRATEG",
    "response#3": "YVYGYSICISTQIGCRMGCKFCASTIDGRVRDLTAGELIGEILAVQKDLGQRISNIVLMGSGEPLDNYENVIKFIKMVNEKYSLNIGQRHITLSTCGIVPKIYDLAREGLQITLAISLHAPNDIIRKQSMPVANSYSIDELIKSCQFYIDKTNRRVTFEYALIKGLNDSPSCAEELSKMLKGLLCHVNLIPINEVKEREYIKADKEIINEFKSILLKNGIEATLRREMGGC"
  },
  {
    "reference": "MDILLILTILIVICALAFDFINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAMTFTGVAKTITKDIVDPYTLENGSVVILAALLAAIAWNLITWYYGIPSSSSHAIIGAIAGAAIAAAGFAALNYKGFIKIIEALILSPIIAFVLGFILYSIVKLIFKDSNLAKTNKQFRRVQIVTAALQSYTHGTNDAQKAMGIITMALITANLHTSANDIPTWVQFACATAMGLGTSIGGWKIIKTVGGKIMKIRPVNGVSADLTGAAIIFGATFIHLPVSTTHVISSSILGVGASHRVKGVNWGTAKRMLITWVITLPISATLGAIAYFILNMIF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization.2. The protein can be expressed in plasma membrane and perform symporter activity without triggering undesirable cellular responses.3. The protein must exhibit the following characteristics: Low-affinity inorganic phosphate transporter.. The designed protein sequence is ",
    "response#1": "PVSVGAALVIVERLPKRLAEAVGIVLELLAGIPSVVVGLWGAMTFGPFIAHHIAPVIAHNAPDVPVLNYLRGDPGNGEGMLVSGLVLAVMVVPIIATTTHDLFRQVPVLPREGAIALGMSNWECVRRVTLPWVSSGIVGAVVLGLGRALGETMAVAMVSGAVLGAMPANIYATMTTIAATIVSQLDSAMTDSTNFAVKTLAEVGLVLMVITLLTNVAARGMVRRVR",
    "response#2": "PVSVGAALVIVERLPKRLAEAVGIVLELLAGIPSVVVGLWGAMTFGPFIAHHIAPVIAHNAPDVPVLNYLRGDPGNGEGMLVSGLVLAVMVVPIIATTTHDLFRQVPVLPREGAIALGMSNWECVRRVTLPWVSSGIVGAVVLGLGRALGETMAVAMVSGAVLGAMPANIYATMTTIAATIVSQLDSAMTDSTNFAVKTLAEVGLVLMVITLLTNVAARGMVRRVL",
    "response#3": "LPLAFLAGAYAYEFPNSFIGRATKMLLQIMLEFPTILVGTFVMGMLVVPMGTFSALAGALALALILTPYVAVYTEEAMAEVPKIYKEGGYALGCTRAQVIFKVITKMAKKGILTGILIGMAKVAGETAPLLFTAGGLYEVYPTNPLEPVGAIPLLIYTLVQSPSIEDHQMAWGAALVMLIIFLAIFL"
  },
  {
    "reference": "MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIAVGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKAYAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELLNSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYDALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMINDAQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNHHMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVLNQPLYQLIEQVKPRLTKAKIKISRKLKRK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Catalyzes the incorporation of the first glycine of the pentaglycine interpeptide bridge, which is characteristic of the S.aureus peptidoglycan; Involved in methicillin resistance .; This glycine is added to the epsilon-amino group of the L-lysine of the membrane-bound lipid II intermediate (GlcNAc-(beta-1,4)-N-acetylmuramic acid(-L-Ala-D-iGln-L-Lys-D-Ala-D- Ala)-pyrophosphoryl-undecaprenol), using glycyl-tRNA(Gly) as donor, in a ribosome-independent mechanism2. We are looking for a protein with broad substrate specificity for beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-   Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + glycyl-   tRNA(Gly) = beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-   Lys-(N(6)-Gly)-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl   diphosphate + H(+) + tRNA(Gly). The designed protein sequence is ",
    "response#1": "MSKKYALDAKYKIKGIGLHTGEFVNITLKPAPENYGYKFQRTDLESNPEILADINYVIDTSRGTTIEKNGAKVYTVEHVLAAIVGLKIDNVLIELNGDEPPIMDGSSDKYVELIEKAGIVEQNADREEYVVSEVISYVDEENNSEIIALPAKEFRVTTMVDFGTKVLGSQFATLENISDFKEEISNARTFVFLHEIETLFNNNLIKGGDLDNAIVIVDKPLSEETVDKLRKLFNKEDIDVKPNGILNNVELRYKNEPARHKLLDILGDLALIGTRIKAKILAARPGHSSNVKFAKILKKEMKKKKKAKVPIYDLNKEPVYDIQEIMKILPHRFPFLLVDKIIEFNEGKYAVGIKNVTINEPFFQGHFPGQPIMPGVLIVEAMAQAGGIMLLNSVEDPHLYSTYFMKIDKVKFKQKVVPGDTVIFELELISPIRRGICHMQGYAYANGKIVAEAELMAQIIKEK",
    "response#2": "MLSLSAVVAKVGLQAAAGNTDVQVTDVSYDSDRIAPGCLFVATRGAQTDGHDYIPDVLARGAVAIVAEREVPAVPGAVVVPDTRRAMAVLAAAAYDEPSATLTLVGVTGTNGKTTVTHLIEAALVAAGRRTGLIGTIENRIGGARLSAQRTTPEAPDLQRLLAEMRDAGCEAVAMEVSSHALELGRVWGCEFDVAIFTNLSQDHLDFHETFENYFAAKRRLFTSDPGAARQRFAVVNADDPWSERFVAETPALVVTYGIERPADVRAENVQLSSSGTSFTLVTPAGTVEVRSPLIGRHNVYNVLAAVAAGVAAGIEPETVAAGLAAVPAVPA",
    "response#3": "MKLLKDILYGVNILEVKGNTNVAVLEITDDSREAGEGTLFVAIKGHTFDTHEYISDVINRGVKAIIVEHEVDVEYGIKVIKINNSRKAMSLMSAKFYNHPTNKLKLIGITGTNGKTTTSYLIKSIFEEANFKCGVIGTINYTIGDKNIDAPLTTPESLDLQRIMNDMVDNDCKYCVMEVSSHSLELNRVQNIDFDVGVFTNLTQDHLDFHKTFDNYYKAKFKLFEKCKYSIINIDDEYGRRMIKELKGKDITTYSLSSKADFYASDIILDEKGTKFVLKTIEGETDFSIKIPGRFSVYNALAAIATAYLEDIKLDDIIDSLNRIKPVK"
  },
  {
    "reference": "MGYKIAIVGATGNVGREMISILDERGFPADEVIALASRRSQGTEISFGDKTLRVQALENYDFTGTDIVLMSAGGDVSKEWXPRITAKGCVVIDNSSAWRYDPDVPLIVPEVNPDAIIGFSKKNIIANPNCSTAQLVVALKPLHDYATIKRVVVSTYQSVSGAGKEGADELFTQTRAVFVSDPVETQKFTKRIAFNVIPHIDAFLDDGSTKEEWKMVAETKKMIDKSIKITATAVRVPVFVGHAEAVNIEFENEITADDARRILREAPGCLVIDKREDGGYITPYECAGEDATYISRIREDSTIENGLNIWVVSDNLRKGAALNSVQIAELLINRGLLKSA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. We require a protein that can specifically bind to the metabolites produced in the Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5 pathway.2. The Acyl-thioester intermediate active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The designed protein must possess NAD binding, NADP binding to support threonine biosynthetic process, isoleucine biosynthetic process, diaminopimelate biosynthetic process, lysine biosynthetic process via diaminopimelate under certain conditions.4. The substrate, phosphate, NADP(+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "LGGLFRENLVEWATSMTYQAASGAGAQNMRELLQQMGEAHRVAGDLLRDPASAILDIDREVAGTLRDERFPTAHFGVPLAGSLIPWIDKDLGNGQSKEEWKGGAETNKILGREANPTPIDGLCVRIGAMRCHSQALTIKLTRDVPLDEIDAIIASANSWVKVVPNEREASMHELTPTAVTGTLAVPVGRLRKMNMGSEYLSAFTVGDQLLY",
    "response#2": "AIAIVGATGSVGEAMLSILDERNFPVGTVHALASSRSVGKRVSFGETHLVVQDLADFDFSQVRIALFSAGAAVSAEYVPKAVEAGCICIDNTSRFRYDEGIPLVVSEVNPERIADDALAKNKGIIANPNCSTIQMVAALKPIHSAVGIERINVCTYQSVSGAGRRAVEELAGQTARLLNGQKITPHVFPKQIAFNVLPRIDQMMENGYTREEMKMVWETRKILGDESIQVNATAVRVPVFYGHSEAVHIETRDKISALQARELLSGAPGIEVVDETDDDGYPTPVTESAGRDGTFVGRIREDISHPRGLNLWIVSDNVRKGAALNAVQIAELL",
    "response#3": "MAIVGATGAVGQEMMKVLAERDFKIDELICLADPREEGTQLKFKDEVITVQKVTEEAFRKADIALFAVGTEISKELAPIAVKNNCVVIDNSYAFRLEEGVPLVVPEVNAEDIKXTPNGFINATCVRVPVMIGHLKPLRDAAGLRRVVVSTYQAVSGAGLAAQQELTDSTQARLRGQSPTPRIFQHNIAFNLIPQIGSHKQDGYTSEEMKMVRETHKIFDDDSIAINATCVRVPVMIGHSESIYCETEQPLDLEEIRQLFRSAEGIALVDDLANKQYPMPVECADRPEVFVGRVRKDLSNPCGIAFWCVSDNLRKGAANPIHKEYGLKRVVVSTYQAVSGAGKAGIDELDEHIKAYMKGEEMKPSVFQYPIAFNLIPHIDVFVEHDYTREEMKMVWETRKIMHLLDLKIAATAVRVPVYRSHSESINLETYKPVTPEGVREVLSRAPGVIVQDNPQEKLYPMPLYTSHKDEVFVGRIRKDISTENGIAMWVVADQIRNPIHKEYGLKRVVVSTYQAVSGAGKAGIDELDEHIKAYMKGEEMKPSVFQYPIAFNLIPHIDVFVEHDYTREEMKMVWETRKIMHLLDLKIAATAVRVPVYRSHSESINLETYKPVTPEGVREVLSRAPGVIVQDNPQEKLYPMPLYTSHKDEVFVGRIRKDISTENGIAMWVVADQIREAAAN"
  },
  {
    "reference": "MALKVIILAAGKGTRMRSRLPKVLQSLAGRPLLAHVVDRAHAIHADEIVTVIGHGSDQVRQTFETAGVRFCEQSEQLGTGHAVQQAVDHLDDADTVLVLYGDVPLTAPETLKDLISLVGSAHPLALLSMTLEEPDGYGRIVRNAHHQVEAIVEQKDASTEQLAIREINTGMLAARGAELKKWLGALDADNAQGEYYLTDIVSMAVADGFEIATTEPERLSEVLGVNNKSQLQTLERLYQREQAERLMAQGVTLSDASRLDIRGQLEVGQDVSLDANVVIEGAVHLGDEVVVEPHCVLKDCRIEAGTRIAAFSHIEGASVGPDCTVGPYARLRPGTVLQQGAKIGNFVETKNARIGDGSKINHLSYIGDTTMGAHVNIGAGTITCNYDGVNKHRTDIGDEVFVGSATQLVAPVKVGSGATIGAGSTITKDVPGDTLTLSRSRQTSLKSWQGPQKKDIDPQT",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein have MobA-like NTP transferase domains with specific sequence motifs that are crucial for its activity.2. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate are properly positioned for optimal catalysis.3. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc); The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.4. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in lipid A biosynthetic process, cell morphogenesis, cell wall organization, regulation of cell shape. The designed protein sequence is ",
    "response#1": "VIMAAGKGTRMKSDLPKVLHRLAGKPLLQHVLDTCAGLGAQRTLVITGHGAPTVEAMLAGGQAASVRQEPQLGTGHAIQQVVPHLGAQGTTLILNGDVPLIAAGTAQALAAACGGDALGLLTVDMPDPTGYGRILRDGNGAVVGIVEHKDASAEQRAITEINTGILAAPAALLKSWLARLTNDNAQGEYYLTDIVALAVADGVPVVSAQPQHAFETEGVNSKVQLAALEREWQQRQAEALLLAGVTLRDPFRVDVRGEVSVGRDIMIDINVILEGKVVIEDDVQIGPNCVIKDSILRRGAVVKANSHLDGAVLGEGSDVGPFARLRPGSVLEAKAHVGNFVELKNARLGEGAKAGHLSYLGDAEIGARSNIGAGTITCNYDGANKFRTVMGEDVFIGSNSALVAPVQLGDGATTAAGSIITQNVEAAQLAVGRARQRPIAGWKRPVKKP",
    "response#2": "MTVVILAAGQGSRMRSRLPKVLHPVGGRPMLHHVMSTARALGAEAIHTVIGHGAEAVRDATPGEATFWVHQAEQLGTGHAVAQALPNLPDDARVLVLYGDVPLTSRETLAHLADQISDRSMALLTVTLDNPTGYGRIERSQGKVLRIVEQKDASEAELTINEVNTGIMAAPTAALRRWLARLTNDNAQGEYYLTDIVAMAVADGLPVHTAQPALAWETEGVNSKVQLAELERAWQHQQAQRLLEQRNAMLGGATLVAPETVFLSYDTVLGRDVIVEPNVVFGPGVRVGDGCTVRAFAHLTETELKAGVRIGPFVRLRGHAVLETGAELGNFVELKNARMGAGAKAAHLTYLGDAEIGAHANIGAGTITCNYDGVLKHRTTIGAHAFIGSNSALVAPVTVGEGAYVASGSVITDDVPADAMAVARGRQRTIKGWQRPTKKK",
    "response#3": "VVILAAGQGSRMRSRLPKVLHPVGGRPMLHHVMSTARALGAEAIHTVIGHGAEAVRDATPGEATFWVHQAEQLGTGHAVAQALPNLPDDARVLVLYGDVPLTSRETLAHLADQISDRSMLTMTLEDPTGYGRIVRNGEGAVDGIVEHKDATAEQRAICEINSGIYCFDAEKLLPHLPELSTDNDQGELYLTDTGATLLDPARIDIRGRVRCGEDVVIDANVTFEGDVELGDGVVIEANVVLKDCRIGSGSRVRAFSHIEGASLGETVNVGPYARLRPGAELADGVQVGNFVEIKKTRMGAGSKASHLTYLGDANIGAEVNIGAGTITCNYDGVNKFVTDIADGAFIGSNSSLVAPVSIGQQATVGAGSTITRDVADGELAVARAKQVNVTGWRRPQKEG"
  },
  {
    "reference": "MAILQDILYKVPLLSVNGNTSIPVTGVQIDSRKVVDGNVFVAIPGEHANGSQFIGKAIAAGAVAIVCELFPDQMEPGITYLQVSNAHEAVAYMAHQFYGEPSKKVKLIGVTGTNGKTTIATLLYKLFSELGHTCGLLSTVQNHIGKEVLPATHTTPDAVSLNALLARMVEEGCTHVFMECSSHAIHQHRITGLHFTGALFSNITHDHLDYHKTFDEYIRVKKSFFDNLPSDAFAISNLDEKRGTVMLQNTVARKYYYSLKNVADFKGKILENALSGLVMIVNDKEVHFRLIGEFNAYNLMAVYGAAICAGEDNDEVLRVMSMLTGAEGRFDYIVSNDKIIGIIDYAHTPDALENVLETIKKLRKGHEQVITVVGCGGDRDKTKRPVMARVSAELSTKLILTSDNPRTEDPQQILRDMEEGLDSAGKRKSITISDRREAIKTAVALANKEDIILIAGKGHEKYQDINGVKHDFDDKAVLLEMFALLNK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. We require a protein that can specifically bind to the metabolites produced in the Cell wall biogenesis, peptidoglycan biosynthesis pathway.2. The meso-2,6-diaminoheptanedioate binding site should be located in a region of the protein that is accessible to the ligand.3. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.4. The protein requires Mg(2+) to activate a critical catalytic site.5. The designed protein should be optimized for magnesium ion binding, ATP binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity activity in order to improve cell division, regulation of cell shape, peptidoglycan biosynthetic process, cell cycle.6. The presence of Mur ligase central, Mur ligase C-terminal domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MKLSQLLTTVPVKKVSGDLSLDVHALHIDSRQIKAGDVFIAIRGLAADGHDFISKAISQGAAAVICETIPDNLADSLPAVTVCAGNPRRAFALAAANFYGHPAQKLKLAGMGVTGTNGKTSVVNFSQQLIEQKNLKTGVIGTIENRIGDVTIPVRNTTPESYTLQKYLAEMLAAGCDTAVMEVSSHALAQGRVDGCDFDIAVFTNLTQDHLDFHKTMEAYFAAKAKLFTPEYLKKAVINIDDEYGQRIVDQTAVPVLTYAIRKDADYYAKNLVYTAGGISGTITTPDGSFPVETRLVGKFNAYNLLAAVGAGVALGIPLEIIKKGIEGAGQVPGRLEQVPAQSGILGVVDYAHKPNAIVAALAALRDLATARGGRLICVIGAGGDRDRGKRPLMGEAAARGADLVIVTDDNPRTEDPAFVRDGVRRGAEDAHAGKVVEVAGRRAAIVEAVRLAATGDVIAVLGKGHEDYQIIGTEKIHFDDREQAAAALMRRI",
    "response#2": "MIKLKDILYKVSINKVIGSTTTAVNEIHFDSRKVKHGDLFVACRGTMSDGHDFINTAVEKGATVIVCEELPDSSTSETTFIVVEDSSATLSQIAHNFYENPSKKLKLVGVTGTNGKTTIATMLFRLFTRLGHICGLLSTVQNQIGENIIPATHTTPDPINLNALLADMVTRGCDYAFMEVSSHAVHQQRIAGLKFAGGIFSNITHDHLDYHKTFDEYIRVKKAFFDGLPATAFALTNLDDKRGNVMLQNTRAHKATYSLRTMADFKGKILENNLTGLIMLVGEREVHFRLIGEFNAYNLMAVYGAASLLLGLDLKEILLILSELKGAEGRMQIVHRQPTVVVDFAHTADALEKLLQALSQHQQDSSLMVLFGCGGDRDQQKRPLMAQVAERYADKVFITSDNPRYEDPQQIIEQIVAGLQKPAEAQIEIDRRSAIRSALEQLSVTENAVLVIAGKGHENYQEIKGVKLNFSDQEEVLKALEEKK",
    "response#3": "MKLSELLKKYEYIVVEGNKNFDINVHALHIDSRQIKAGDVFIAIRGLAADGHDFISKAISQGAAAVICETIPEKYAKKIICVDSKNALAIISSIFFGNPSDKIKIYGVTGTNGKTTIATMLFRLFTRLGHICGLLSTVQNQIGENIIPATHTTPDPINLNALLADMVTRGCDYAFMEVSSHAVHQQRIAGLKFAGGIFSNITHDHLDYHKTFDEYIRVKKAFFDGLPATAFALTNLDDKRGNVMLQNTRAHKATYSLRTMADFKGKILENNLTGLIMLVGEREVHFRLIGEFNAYNLMAVYGAAGLVLGIEKDEILKNLESIKGVAGRFQYTVSTEGVTAIVDYAHTPDALKNVLQTINDIRTGNETVITVIGCGGDRDVAKRPKMAMIASQLSDQVIFTSDNPRTEDPQSILDQMEVGVAAENYKKTLSILDRRQAIKTACKLAKVGDIILVAGKGHETYQIVGDEKREFDERKIAREAIKER"
  },
  {
    "reference": "MTHITFDYSKVLGQFVGEHELDYLQPQVSAADAFLRQGTGPGSDFLGWMDLPENYDKEEFSRIQKAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNNHFANLQTAEERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFKELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLLPIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEPSLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVVRVEKPRKNVTIPELTEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPTQDAYTLGYTIYFFELAIGLSGYLNSVNPFDQPGVEAYKRNMFALLGKPGFEELSAELNARL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must be able to perform carbohydrate derivative binding under specific conditions.2. For general function, the protein need meet that Catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate.3. The protein should be designed to catalyze the following reaction alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate.4. We require a protein that can specifically bind to the metabolites produced in the Carbohydrate degradation pathway. The designed protein sequence is ",
    "response#1": "MVVLPYKDRLLLFSRYLQQLIMESIGKELDLDGTVVNQGLTVYGNKGSTDQHAYVQQLREGTNNFFVAFIEVLKDREGASLAVEPGFTSGDYLSGFLQGTRTALSEKGRESLTITIPAITPRTVGVLVALFERAVGLYASLVNINAYHQPGVEAGKKAAGGWLAACVIEN",
    "response#2": "MVVLPYKDRLLLFSRYLQQLIMESIGKELDLDGTVVNQGLTVYGNKGSTDQHAYVQQLREGTNNFFVAFIEVLKDREGASLAVEPGFTSGDYLSGFLQGTRTALSEKGRESLTITIPAITPRTVGVLVALFERAVGLYASLVNINAYHQPGVEAGKKAAGGLVGLHFYPIWEATSLDEWLYNGGPYQLVVFHFLIAIATYMGREWELSYRLGMRPWISVAYSAPVAAATAVLLIYPIGQGSFSDGMPLGISGTFNFMLVLQAEHDWVGGRFSLWSAISLNEETTINSYKFGQEDETYSIVAAHGYFGRLIFQYASFNNSRSLHFFLAAWPVIGIWFTALGISTMAFNLNGFNFNQSVLDSQGRVVNTWADILNRAGLGMEVMHERNAHNFPLDLAAGEATPVALAAPTIG",
    "response#3": "MVVLPYKDRLLLFSRYLQQLIMESIGKELDLDGTVVNQGLTVYGNKGSTDQHAYVQQLREGTNNFFVAFIEVLKDREGASLAVEPGFTSGDYLSGFLQGTRTALSEKGRESLTITIPAITPRTVGVLVALFERAVGLYASLVNINAYHQPGVEAGKKAAGGLQAFTAA"
  },
  {
    "reference": "MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPGSLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWLRRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMPNNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVAEEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGEAAAS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The desired protein should be able to perform H2O + P(1),P(4)-bis(5'-adenosyl) tetraphosphate = 2 ADP + 2   H(+)2. The ideal protein would be one that plays a critical role in response to X-ray through phosphatase activity, bis(5'-nucleosyl)-tetraphosphatase activity within cytoplasm.3. The protein must exhibit the following characteristics: Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP.. The designed protein sequence is ",
    "response#1": "MSTYFIGDIHGCFNELMHLLEKVSFDANSDVLWLTGDLINRGPKSLEVLRFVSSLGDNVKMVLGNHDVNLIALYASIKNSKKSSLMNNLLKSHDIDYLIYWLRKQPLFRVDHKKKIIMSHAGMAG",
    "response#2": "MQFDIIGDVHGCYDELMELIDKLGYEYKRGSYDHPDGRLLAFVGDLTDRGPHSLEVIRLVSHMVKKKTAYYVPGNHCNKLYRYMIGRKVQVKHGLETTVAELNALSEEERIEVIAQFKELYEQAPLYHSFPEDKLVITHAGIREDDIGSYGKRVETFVLYGDITGETNPDGTPVRRDWAATYQGDWWIVYGHTPLLNVHRTSLEDIIGDVHGCYDELMELIDKLGYEYKRGSYDHPDGRLLAFVGDLTDRGPHSLEVIRLVSHMVKKKTAYYVPGNHCNKLYRYMIGRKVQVKHGLETTVAELNALSEEERIEVIAQFKELYEQAPLYHSFPEDKLVITHAGIREDDIGSYGKRVETFVLYGDITGETNPDGTPVRRDWAATYQGDWWIVYGHTPVRRPRIIHRTVNIDTGCVFGGALTALRFPEIEFVSIPSRQYAVGDLQGCLEPLKCLLQQVAFDPALDRLWLVGDLVNRGPQSLETLRFLYGMRESLVCVLGNHDLHLLAAAKNIERLKKSDTLREILEAPDCAELMEWLRQQKLMHYDEQREVAMVHAGIPPQWSLRKALKYAEEVEQRGVVVW",
    "response#3": "MAVYAVGDLQGCLQPLKCLLEQVAFDPAQDQLWLVGDLVNRGPQSLETLRFLYSIRHALTCVLGNHDLHLLAVAHNIERLKKGDTLREILDAPDRDDLLDWLRLQKLVHHDAERQITLVHAGIPPQWSLAKALKRAAEVEEVLRDDARLPLFLDGMYGNEPAS"
  },
  {
    "reference": "MVGVLLSERNGSQKRHCSSISSDDGRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLACSSCCPKLRNKCPACALPVGHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKICNFSPCSCPVQGCNYTGSYKDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVYEKKLLFVVQCFEEPCGVYVSVSCIAPSAPEVGEFSYGLLYTTWEGVTMTYQSPKVKKVLKVSSQRPKDSFMLIPHSLLCGPLLGMMLCINELKQM",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.2. For general function, the protein need meet that It probably triggers the ubiquitin-mediated degradation of different substrates.3. The protein should be designed to catalyze the following reaction S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +   [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-   cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.. The designed protein sequence is ",
    "response#1": "LTTAAPGYTCPLQGSHAICTCCFQPMPDRRAEHEQDSRIAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLTTRGLTWKNMLTESLLALQRGVFILSDYRITGNTVLCYCCGLRSFRELTYQYRQNC",
    "response#2": "DEAERRREMAKNSLERYTHYYERWASNQVSRQKAMGDLQKMQSEKLGKLSDIQCTPESQLKFIAEAWLQIIECRRVLKWTYAYGYYLQDHAKKPFFEYLQGEAESGLERLHKCVEKDIEVFELAEGPSEEFNHFRTKLTGLTSITKTFFENLVKALENGLAR",
    "response#3": "DEAERRREMAKNSLERYTHYYERWASNQVSRQKAMGDLQKMQSEKLGKLSDIQCTPESQLKFIAEAWLQIIECRRVLKWTYAYGYYLQDHAKKPFFEYLQGEAESGLERLHKCVEKDIEVFELAEGPSEEFNHFRTKLTGLTSITKTFFENLVKALENGLAV"
  },
  {
    "reference": "MCGAGTGRELLVADTHRVSWASAAALCGRPCDARDFGHGSLVCACSATYCDTLDPLVLPAPGTYIKYESSKAGKRLERSQGSFQHNTKTPDFHLTLDTAQRYQKVKGFGGSVTDSAAINIQSLSKDAQNHLLRSYFSEEGIEYNLVRVPMASTDFSIRLYTYADAEGDFELKHFNLTEEDTRMKIPILQAAQKVAKRPLSLYASPWTSPVWMKTNGAMTGRGTLKGSPGDKYHQTWAKYFIRFLDEYAKYNLTFWAVTAGNEPTAGEIVFYPFQCLGFSPEHQRDFIARDLGPALANSSHRDVQLIILDDQRVMLPYWAQVVSSHGLHWLLLLGAQGGAGWLGPWEQVQPQHPDGRALRGPLTPIIVDSSKDIFYKQPMFYHLGHFSKFIPEGSQRVGLTVSKKCRRCDLEHSAFLRPDGAVVLVVLNRSPVDVSFGISDPQLGFIEATAPSDSIQTFLWKQPA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be optimized for glycosyltransferase activity activity in order to improve sphingolipid metabolic process, cholesterol metabolic process.2. Ideal protein should have a binding affinity for key enzymes in the Steroid metabolism pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MKGFGGSLSDAAAMNILRLSQPAQDNLLRSYFSESGIEYNLIRLPMACSDFSVRPYSYDDVPYDYELKHFKLVDEDVKMKIPLLHRALAMSKRPLSLYASPWTAPGWLRSNGDVRGKGTLKGQAGDKYHQIWANYFIKFLDEYAKHNVTFWAVTAQNEPLAGLFTPPQMPTIAFTAAQQRDFILRDLGPALARSPHRTQLLILDDQRIHLPHWAKVVLGNATAARYVAGLGVHWYLDAIVPPGCSLEATHKLFPDHFLLYTEACSGFLTFRFAVSLGCWERGDQYSHSILTVLNHFVAGWTDWNLVLDLEGGPNWVKNFVDSPVIVDSSKDVFYKQPMFYHLGHGSQRVGLVASRSCKKCALEYTAFLRPDGAAIVVVLNRSLRSVDFRLVDNGRVIKATSPAQSIQTL",
    "response#2": "LKGFGGSLSDSAAMNILELSQPAQDNLLSSYFSESGIEYNLLRLPMACSDFSTRPYSYDDVPDDYELKHFRLADEDVKMKIPILQRASAMSKRPLLLYASPWTSPAWMKSNGDVRGKGTLKGQAGDKYHKTWANYFIKFLDEYAKHNVTFWAVTAQNEPLAALFTPSEFPTIAFTAAHQRDFIIHDLGPALAQSSHPTQLIIMDDQRINLPHWAKVVLGNATAARYVAGIGVHWYLDSIVPPGCSLEATYKLFPDHFLLYTEACSGFLTLRFSVSLGCWDRGERYSHSILTVLNHFVAGWTDWNLALDLKGGPNWVKNYVDSPIIVDSSKDVFYKQPMFYHLGHFSKFL",
    "response#3": "VKGFGGSVTDAAAINILSLSQKVQDNLLRSYFSEEGIEYNLIRMPMASCDFSVYPYTYDDVPYDYDLVHFRLKHEDTKLKIPVVHRAQAMSKRPISLCASPWTSPTWMKTNGDVKGKGSLKGSAGDKYHKTWANYFVRFLDEYAKHNLTFWAVTAENEPTAGLIRNYPFQCLGFTPEQQRDFIALDLGPALANSSHKGIQLIILDDNRIQLPHWAKVVLGDERAARYVNGIGVHWYLDFLAPIEDTVLATHHLFPNYYILATEACVGSKFWERPVVLGCWDRGNMYSHSILTNLNNYVTGWTDWNLALDLEGGPNWVKNFVDSPIIVDSRENIFYKQPMFYHMGHFSKFIPEGSQRVGLVASRESKKTDLEYTAFLRPDGAVVVVVLNK"
  },
  {
    "reference": "MDNHLQRLKSFQVVELCEDSRRAGPQRAFVAVPPATSGSRDGRDYAHSALELGAPLVLGEGEAPPGFPPDRWLRCEPIRQLYPRLAAALAGNPSAALSLVGVTGTDGKTSVSWLLSHLLELLDGQPCGQIGTLGWRWGQERRPSDFTTPPAAQLQPGLSEMVSDGVQAAVMEVSSHAIVLGRVEQLDFSVAALTTLGRDHLDFHGDLASYHGVKKSFLAAQSRHCRLVLPVGVSIDLPAQQSVIRVGSGGDFYQQRMGPQTWRLHTPSGVVDRRLPLHADFQVDNLLTALAIAHGLGRSLDDLLPLCSELPPIPGRLEAVIPGAVVDFAHTPEALTTLHQQLRPATSGRLITVFGCGGDRDQGKRPAMARAVEQSADLIILTSDNPRSEDPEVILDQTQAGFTAAPLDADQLTSDSQGWLRVTDRREAIALAWRQRQPHDLVLVAGKGAETHQEIKGQRHPFDDRQELRRLMERPC",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be designed to have high stability and activity towards ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.2. The designed protein should have a high specificity for ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding, with minimal off-target effects.3. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..4. The designed protein should contain one or more Mur ligase central, Mur ligase C-terminal domains that are essential for its function.5. The protein design should include a Meso-diaminopimelate recognition motif motif to ensure proper function. The designed protein sequence is ",
    "response#1": "MKLTDLLKGMPVTAEEGDPEEISGIEIDSRKVKQGDLFVCVTGKTGIADRHLYAAQAVSNGASAIVVEHDVDLAGLAEQCVPYFKADSRRVMGLLASRFYGHPSAKLALIGVTGTNGKTSITFILEHLLTRAGVKTGLIGTIENRIAGQTVPASNTTPESLTLQEYYRQMLDEGCEYAVMEVSAHAVKQNRVYGLDFEAGVFTNLTQDHLDFHGNMESYFQAKKKLFTEPGCRRAVINRDDPAGRELIGMCPEQVLTYGLEKEADLRAEEIKISAEGTRFVLDFAGRTYDCQLPLLGRHNIYNCLGAVGAALHFGIPMENIKKGLQKAKGVKGRMEAFHASGKPLVVVDYAHTPDALQQVLQATRRHCRGQLWCVFGCGGDRDRGKRPQMGQVAAQLADQIVVTDDNPRTEPPEQIIKDILAGIPQQQHVRVQLGRKVAVLDAIANASADDIVVLAGKGHETYQIFKDRTIHFDEREVVAEALREQ",
    "response#2": "MKLRQLVEGLDVEQIHGNADPEEISGIEIDSRKVKQGDLFVCVTGKTGIADRHLYAAQAVSNGASAIVVEHDVDPERGIRHVQVRNTRQAMALVSARFFGHPAQKLNLIGVTGTNGKTSITFILEHLLTRAGQKVAMIGTIGVRIGDRIIPADRTTPEAPDLQKVFSQLVEAGCEYCAMEVSSHALDQGRVNGCDFEVAVFTNLTQDHLDYHGTMEEYAKAKSLLFSRLGNQSAGSKKPAVAVLNADDPASRSMAGLITGTQGKTTSAYLAASALRACGDQVGAIGTTGAFLGGRPVASALTTPEAPALHALFAVMEAHGADAVVMEVSSHALALHRVDGVVYDVAVFLNLGRDHLDFHADVEDYFAAKAALFTPERAAQAVIDIDDPYGRRLADATSLPVTTVSLEGRVEAHWRVVALETTASGSRARVVGPDEVEHDLVVRLPGRFNVANALAVLAATGMALGFTDAQILAGLAHLKGVPGRVEPVKVSDKFILMIDYAHNAMSLKSILETLREYNPKRLVSVFGCGGNRSKLRRYEMGEVSGKLADFTIITSDNPRFEEPLDIIADIRSSIEKTGGEFIEIPDRKEAIRYAIEHGREGDIIVLAGKGHETYQEIGGVKHAFDDREEAEQFLRCRL",
    "response#3": "MKLTELLKGLTCRILEGNLDVDEISGIEIDSRKVKQGDLFVCVTGKTGIADRHLYAAQAVSNGASAIVVEHDVDGQDEITQLVCDSRKIVEGCVFVCVKGTVFDGHTFIDEAINMGAAAIITEEQVEIIGVTGTNGKTSITFILEHLLTRAGEFPAAIGVTGTNGKTSITFILEHLLTRAGYKTGLIGTIQMKIGDHEVAGKNTTPESVDLQRLLAEMVAQGCEYCVMEVSSHALELSRVYGCEFKAAVFTNLTQDHLDFHKTMEAYFSAKAKLFELLGKSGRVEPVKVSDKFILMIDYAHNAMSLKSILETLREYNPKRLVSVFGCGGNRSKLRRYEMGEVSGKLADFTIITSDNPRFEEPLDIIADIRSSIEKTGGEFIEIPDRKEAIRYAIEHGREGDIIVLAGKGDEDYIVKELAGTISFDLGTSGRIENAVPLPGKFNVDNALAAIACSRLAGISEEDIAAGLEKAAVPGRMEPVDEGQDFTAIVDYAHKPDALRAVLSSLRGLGDGRLLVVVGAGGDRDPGKRPLMGAVAAELADVVVVTDDNPRSEDPATIRAAVLEGAREAGAAAELVEIGDRRGAIVRAVAQARPGDVVVVAGKGHETYQILRDRKIPFEEREIVRELVGKEI"
  },
  {
    "reference": "MIIESRIEKGKPVVGMETTVFVHGLPRKEAIELFRRAKEISREKGFQLAVIGILKGKIVAGMSEEELEAMMREGADKVGTREIPIVVAEGKNAATTVSATIFLSRRIGIEVVVTGGTGGVHPGRVDVSQDLTEMSSSRAVLVSSGIKSILDVEATFEMLETLEIPLVGFRTNEFPLFFSRKSGRRVPRIENVEEVLKIYESMKEMELEKTLMVLNPVPEEYEIPHDEIERLLEKIELEVEGKEVTPFLLKKLVEMTNGRTLKANLALLEENVKLAGEIAVKLKRS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The stability of the protein-Mn(2+) complex should be optimized to maximize enzymatic activity.2. The protein's architecture should predominantly feature strand-strand-helix-helix-strand-strand-helix-strand-helix-helix-strand-helix-strand-helix-strand.3. The designed protein should have a high specificity for substrate binding, with minimal off-target effects.4. A protein with metal ion binding functions is required to enable nucleobase catabolic process.5. The designed protein is the optimization of the Proton donor to achieve high catalytic efficiency for D-ribose 5-phosphate + uracil = H2O + psi-UMP. The designed protein sequence is ",
    "response#1": "VVDHPLIKHKLTIMRDKNTGPKEFRELLREITLLLAYEATRHLKCEEVEVETPITKTIGYRINDKDIVVVPILRAGLVMADGILELLPNASVGHIGIYRDPETLQAVEYYAKLPPLNDDKEVFLLDPMLATGVSSIKAIEILKENGAKKITLVALIAAPEGVEAVEKKYEDVKIYVAALDERLNDHGYIIPGLGDAGDRLFRF",
    "response#2": "TVLHTNDHHGHFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKRQDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHMGHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHG",
    "response#3": "VVDHPLIKHKLTIMRDKNTGPKEFRELLREITLLLAYEATRHLKCEEVEVETPITKTIGYRINDKDIVVVPILRAGLVMADGILELLPNASVGHIGIYRDPETLQAVEYYAKLPPLNDDKEVFLLDPMLATGVSSIKAIEILKENGAKKITLVALIAAPEGVEAVEKKYEDVKIYVAALDERLNDHGYIIPGLGDAGDRLFRF"
  },
  {
    "reference": "MTCIVFGVSGGIAAYKAVEVVRRLAERGFDVRVVMTEHATRLVGPDTFRAVSGNPVSLRLFDAQGKAMEHISLARAADVVVVAPATANILAKMAHGLADDLLSTTLLATRAPVLVAPAMNREMYRHPATQENLRKLRERGVQVVGPESGPLACGEEGEGRMAEPPAIVEAVLRLLGARGDLSGHRVLVTAGGTREPLDPVRFIGNRSSGKMGYALAETARELGAEVILVSGPTQLQAPPGVERVMVETAEEMRREVIARCTGCSVVAMAAAVADFTPSRRSEEKIKRAVREGLTVELVPTPDILGELRERKPPGQVLVGFAAETGDLLTKARKKMEDKGVDSLVANDVSRPGSGFDSDYNQAVILFRDGREMELDLMPKRELALRIWEAVAGLLGDRAIK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for CTP binding.2. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A. The designed protein sequence is ",
    "response#1": "MNILLGVSSGIAIYKAVDLVSKMRKQGWNIQIIMTENAAKLVNPIVFSAVGDCKVYTDTYEIEAGWIIHTELSKWADVLLVAPATANTIAKLANGIADNLLTTTALAFAKDKRIIVPTMNTRMYENELTLENIEKMRQHGWHVLEPESGHLACGDVGKGRYPENEKIIE",
    "response#2": "LRGRRVLVTSGPTHEPIDPVRYIANRSSGSQGTAVARALAGLGAEVVFVTGPADVPPPEGVQVVRVQTAQQMAEAVHDALPVDAGVFAAAVADWRVASASDRKLKKTRDGMPKLEFAENPDILASVSRMTRGRPGLVVGFAAETDDVLDNATAKRLRKGCDWIVANDVSPATGIMGGSENAVVLISVADGEVKVPDMSREELAEELIKIVDGIA",
    "response#3": "MNILLGVSSGIAIYKAVDLVSKMRKQGWNIQIIMTENAAKLVNPIVFSAVGDCKVYTDTYEIEAGWIIHTELSKWADVLLVAPATANTIAKLANGIADNLLTTTALAFAKDKRIIVPTMNTRMYENELTLENIEKMRQHGWHVLEPESGHLACGDVGKGRYPENEKIIEW"
  },
  {
    "reference": "MEDISSRNIDELKNYAASLGEKPFRAKQIYKWLHQSLSESFEEMTDIPKALRTRLSNQAYITAPEKVEVQISKEDGTRKYLFRLPDDSLVETVLMRYKYGLSVCISSQVGCRMGCAFCASGMNGLARGLTPGEMLGQIEAIQRDIFNETAGAVPKPDSEEYLRNRISHVVVMGTGEPLDNYDNLLSFIRLLTDENGLHLSQRNLTVSTCGIVPKINELAEKKLEITLALSLHAPTQEKRKKLMPIANRYDIHEAVDAMKSYQEKTGRRVTFEYSLIKGVNDTDEDAEELSELLWRGAHMNLIPVNPVKERSFAAPTRAAAMDFQRKLEKFGINATIRREMGQDIDGACGQLRRRHLER",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.2. A key design requirement for the protein is the optimization of the S-methylcysteine intermediate to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.3. The presence of [4Fe-4S] cluster is necessary for the protein to function in the desired environment.4. The protein contains novel Radical SAM core domains that confer a unique function or activity.5. The ideal protein would be one that plays a critical role in rRNA base methylation through rRNA binding, 4 iron, 4 sulfur cluster binding, rRNA (adenine-C2-)-methyltransferase activity within cytoplasm. The designed protein sequence is ",
    "response#1": "STQVGCAMGCRFCRTAGGGLVRNLEAGEIVAQIIAAQKYCPELGRVTNVVFMGMGEPLHNYDAVVRAFSIITSDWGISISRRKVTVSTSGLVDAIQKLPHSMLMNLAVSLNATTDEQRSAIMPVNLRWPIAELLKTLATLELPDREHFTIEYVLLGGFNDTLEDALLAHLEQKTACVSSQIGCSLSCKFCATGYMERERNLEFDEIYDEVVLINQQSERIYDKKLSNIVFMGMGEPLLNYKNVLRAIEKISSPDGLGMSQRRITVSTAGVAKMIRQLGDDQVRFKLALSLHAANDQKRHAIMPINDTNNIRSLIDALNYFYKQTGNEITFEYILFRNFNDSTKDADELVKIYRQVPADLVNIIEYNPIDKALFEKPDDETVEKFMNKESL",
    "response#2": "QAGRGTLCVSSQAGCALDCSFCSTGKQGFNSDLTSAEIIGQVWIANKSFGTVPGKIDRAITNVVMMGMGEPLLNFDNVVTAMSIMMEDFGYGISKRKVTLSTSGVVPMIDKLGETTDVSLALSLHAPNDELRNQLVPINKKYPLKMLLDACRRYITRLGKERVLTIEYTLLSNVNDQPEHAEQMIALLKDFPCKINLIPFNPFPHSGYERPSNNSIRRFQDMLHKGGFNVTVRTTRGDDIDAACGQLAGQVQDRTRLDEAARAAAA",
    "response#3": "QAGRGTLCVSSQAGCALDCSFCSTGKQGFNSDLTSAEIIGQVWIANKSFGTVPGKIDRAITNVVMMGMGEPLLNFDNVVTAMSIMMEDFGYGISKRKVTLSTSGVVPMIDKLGETTDVSLALSLHAPNDELRNQLVPINKKYPLKMLLDACRRYITRLGKERVLTIEYTLLSNVNDQPEHAEQMIALLKDFPCKINLIPFNPFPHSGYERPSNNSIRRFQDMLHKGGFNVTVRTTRGDDIDAACGQLVGQVMDRTGRARDMAGRGL"
  },
  {
    "reference": "MARTVADLLALTLADAGVERIWGVTGDSLNGLNDSLRRLGRIEWMHVRHEETAAFAAGAEAAITGKLAVCAGSCGPGNLHLINGLFDCQRNHVPVLAIAAHIPSSEIGLGYFQETHPQELFRECSDFVELVSSPSQMPEVLHRALNTAVGRGGVAVVVIPGDVALAEAPAGASARWAAPLPPRILPADAELDRLAALLDGSEAITILAGSGCAGAHDAVVDLAARLRAPIVHALRGKEHVEWDNPYDVGMTGLIGFSSGYHAMMSSDLLLMLGTDFPYRAFYPRDAKIVQVDRDPGALGRRATLTQGIVGDVGETVAALLPRLKPRSDEAFLSTALKHYAKAREGLDDLAVPDKPGRPIHPQYLTRLVSEIAAGDAIFTADVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMLQAIGAQAAAPDRQVVSLSGDGGFTMMMGDFISLTQLGLPVKVIVFNNGSLGFVAMEMKAGGYLDSGTDLENPDFAAMAAAMGVKSFRVTESADLEGVLREALAHPGPVLVDVVTAKQELVMPPKIQLEQAKGFSLYMLKAIISGRGSEVVELARTNFRS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that A peripheral cell membrane enzyme that catalyzes the oxidative decarboxylation of pyruvate to form acetate and CO(2) The designed protein sequence is ",
    "response#1": "VYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPFRSKVLKDLPRIEGRPGASLPPLNLKELEKDLIDRHGEEVTPEDVLSAAMYPDVFAQFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIL",
    "response#2": "DALAAMIDAGKRVTLMCGSGCAGAHDEVVALAARLKAPVVHALKGKEYVEYDNPYDVGMTGLVGFASGYYAMMDADMLLMLGTDFPYRQFYPSGSARIAQVDIRAGQLGRRAAIDLGVVGDVGATIRARAIAIAAGVPVVPGTNSPINSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPATHLDPQLRSRLTSDSVKLAKQVGYENAGTVEFLVDKHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVSA",
    "response#3": "LRAILTGSACVRPASVYDAISIRIADDLGFPFGMFGGSVASLAILGDPDIALITLTELAEQVRRMSRAAALPVVVDADHGYGNALNVRRTVEELEAAGAAGLTIEDTLLPQAYGEAKPQLVSREEGLGKITAALDARRDPNLVIIGRTGACSITSLDDAIERALAYQAAGVDALFFTGVKTRAQLEAISAATTLPIALGSPPAELGDFDHLAERRVRIAVQGHAPIAAATEAVFKTLSY"
  },
  {
    "reference": "MDDIVVAGISGRLPESDNLEEFWENLINGVDMVTEDDRRWKPGLYGLPKRNGKLKDVSHFDASFFGVHPKQANTMDPQLRIMLEVAYESIVDGGLNPSTLRGSKTGVYIGVSGSEASEAFSRDPEELLGYSMTGCQRAMLANRLSYFFDFSGPSTAIDTACSSSLLALENAFHAIRQGHCDAALVGGVNLLLKPNTSVQFMKLGMLSPDGTCKSFDSSGNGYCRSEAAVAVLLTKRSAAKRVYATVVNAGNNTDGYKEQGVTFPSGEMQQKLVCSLYQEANICPEEVEYIEAHGTGTKVGDPQEVNGIVGVFCQSKREPLLIGSTKSNMGHPEPASGLAALAKVLLSMEKGVWAPNLHFNSPNPDIPALTDGRVQVVDRPIPVRGGIVGINSFGFGGSNVHVILRPSKRSDKKPADVTDKLPRMLQACGRTDSAVQTILQKAKEHIEDHSFLSLLNDVSAAPPSSMPFRGYAVVGAQSDVMEVQQVQNSSRPLWFICSGMGTQWAGMGRSLMLLPDFKESVLRSDKALKGTGLVVSQLLMEANDATFEDTVHAFVGLAAIQIAQIDLLSKMGLQPDGIVGHSVGELACGYADGSLSHSEAILAAYWRGRCIKEANLPPGGMAAVGLTWKECMEQCPEGVVPACHNAEDTVTISGPQEAITKFVSELKQQGVFAKEVRSAGVAFHSYYMASIAPTLLAALKKVIKEPRQRSSRWVSTSIPQSEWDSPLAQYSSADYHVNNLVSPVLFQEGLRLVPDNAVVVELAPHALL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein can be expressed in membrane and perform myristoyl-[acyl-carrier-protein] hydrolase activity without triggering undesirable cellular responses.2. For general function, the protein need meet that Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH3. The protein should have a fast turnover rate for holo-[ACP] + malonyl-CoA = CoA + malonyl-[ACP].4. A protein should be able to manipulate the activity of enzymes in the fatty acid biosynthesis, Lipid metabolism pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "FIIIAQADGLPQDDFNDIFTKDAAFWVYGNSKVLYANRISFLFDFQGPSLVIDTACSASLVALATAMNDIRMGVCDTAVVATGNLVPAPFGNSIYSCVGLLASDGKCKVWDKKADGFVRSETIGALILQRRSQAKRVYATVLHSKVNSDGFKSVGLFAPYWLRQKDLMVSTYEEAGVDPNDLVYFEAHGTGTNVGDPQEAKAIAEAYCKHREQPLLVGAVKSNLGHSEGSSGLNSVAKVIIAFENKCIPANLHFNEPKQEIYAIVNKQIEPVIKNTSFPAGIVGVNSFGVGGVNAHILLKPHDDVDAVRDGRIEVVHENTPFKRRFAAVNSFSYTGPMAHVLLKGHYKEKNVEKYITNIPYLVNVSSRTEDNVGNMLDILAAMPIDAEYIGLLHNIHKIVTSGHTVRGYIYPNRISYWFDSKSPSYYVDCGCVGSMAVVHLAFNSIVAGECEAALVGGCSLCLSSTVTLNLINAGLLCNDGKTKCFDKRADGYVRSETIGFLFLQKAKDAKRIYAEIYHTKLGYCSSTLNNGQIVRHSNSKDIIKFLKKFYSEIDVSPQEVEYVEANGIANAEDDKNELEAIGEIFGNKERAVKIGCVKSNMGNSESASGLCALTKVCLAYRNGELSGNLHYENPHDDVDAVRDGRIEVVHENTPFKRRFAAVNSFSYTGPMAHVLLKGHYPRSDVASLHDGRMQIVTANTPFNRTSYTAVNGISYTGLNGHALLQSCFKPKDLTKYKSNIPYLVTVSGRHEAAVEKILDDIKDRPLDPEEIALLHNIHKNRISKHLARG",
    "response#2": "MEEVVIAGMSGKLPESENLEEFWANLIGGVDMVTDDDRRWKAGLYGLPKRTGKLKDLSKFDTSFFGVHPKQAHTMDPQLRLLLEVAYEAILDGGINPASLRGTHTGVWVGVSGSEASEALSRDPENLLGYSMVGCQRAMMANRLSFFFDFKGPSITLDTACSSSLLALQSAYQAIRNGECPAAIVGGINILLKPNTSVQFMKLGMLSPEGCCKSFDESGNGYCRSEAVVAVLLTKKSLARRVYATILNAGTNTDGFKEQGVTFPSGDVQEQLIRSLYEPAGLDPESLEYIEAHGTGTKVGDPQELNSITRALCATRRDPLLIGSTKSNMGHPEPASGLAALAKVLLCLEHGLWAPNLHFHSPNPEIPALLDGRLQVVCQPLPIRGGNVGINSFGFGGSNVHVVLQPNPPARLTRSVPRVLSPRSDVASLHDGRMQIVTANTPFNRTSYTAVNGISYTGLNGHALLQSCFKPKDLTKYKSNIPYLVTVSGRHEAAVEKILDDIKDRPLDPEEIALLHNIHKNRISKHLARGPHDDVDAVRDGRIEVVHENTPFKRRFAAVNSFSYTGPMAHVLLKGHYKEKNVEKYITNIPYLVNVSSRTEDNVGNMLDILAAMPIDAEYIGLLHNIHKIVTSGHTVRGYILSRNPEAVVISGVSGLLPDCDSYVEFMDKIFKNENLVKANPRWGDFKYPELPELYGRISGLDRFDAQFFTVFYTLAQAIDPTARKIIEHTYQAIFDSGINPKELWGKNIGVFVSYGYSETEKLTYTNDMQLAVLGSCKAMTANRISYWLNLKGPSIYMEGSSCGGLLALEKAREAIVGGICEAAIVGVGKLVLHPQTPISHRGMLNTDGITKCYDDAANGPSMSEAVNVFFLQKYKDANRAYVVLRHAKTQFMAIDEIISTEHCEIIQNKEQLTKFFHQFYEEAGVSPENVEYIEGYGAASPKMDKMELQAMENVFCKDRINPLLVGSVTSNLGYTESATGSCAIFKLLTAYQRGEIPATINCRNPRSDVASLHDGRMQIVTANTPFNRTSYTAVNGISYTGLNGHALLQ",
    "response#3": "MEEIVVAGISGRLPESNNLEEFWENLFNGVDMVTEDDRRWKPGLYGLPKRNGKLKDISRFDAAFFGVHPKQAHTMDPQLRLMLEIAYEAIVDGGLNPTELRGSRTGVYIGVSGSEAGEAFSRDPEELLGYSMTGCQRAMFANRLSYFFDFNGPSTAIDTACSSSLLALENAFNAIRHGHCDAALVGGVNLLLKPNTSVQFMKLGMLSPEGTCKSFDSSGNGYCRSEAAVAVLLTRRSMAKRVYATVLNAGNNTDGYKEQGVTFPSGEMQQRLVRSLYQEVNITPDQVEYIEAHGTGTKVGDPQEVNGIVSVFCHSKRDPLLIGSTKSNMGHPEPASGLAALAKVVLSLERGVWAPNIHYNGPNTDIPALSDGRLRVVNEPIPVRGGIVGINSFGFGGSNVHVILRPNQKSQTPKDSEVPRLLQACGRTEAAVTTILQKGREHKEDSAFLALLNEVSGVPTSSMPYRGYALVGSQSDVFSGMGSQWLTMATSMMALEPFARSIHQSAAVLKPLGIDLIELLTKNDPELWKSTVNPFVAITSMQVALYDVIKLLNIKIEGIIGHSFGEVACAYADGCLTLEQAILTSYWRGKIVEQSKLPRGVLAAIGLSWEETKKRCPPGIVPACHNGSDSVTISGLYEETKKFVEKLQAENVFARLVAGGEYPYHSPHMNAVAPDLIAALNKVIPEPKKRSSKWISTSFDKSNWFIEDACYASAKYFTNNLVSPVLFNEGMQEISKDCIAIEVAPHSLFDSIFKRCYQQHHYIGLMKRN"
  },
  {
    "reference": "MNLLHLDSSILGDHSASRQLTHEVVQAYLTAHADSQVTYRDLASDALGHFSAASLAAAGTPVEVRDAAQQQEVAGNEATLQQFLDSDVLVIGAPMYNFTIPTQLKAWFDRILIAGRTFRYSEAGPEGLCGGKKVIIVSTSGGLHAGQPTGVGHEELLKALFAFIGITDLQFVRAHGLAYGEEPRASAMSAAQHYIQNELFAA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The presence of FMN is necessary for the protein to function in the desired environment.2. The protein must have high specificity towards FMN binding, oxidoreductase activity, acting on NAD(P)H, NAD(P) as acceptor, oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor, electron transfer activity.3. For general function, the protein need meet that Catalyzes the reductive cleavage of the azo bond in aromatic azo compounds to the corresponding amines.; Also exhibits azoreductase activity4. The protein should be designed to catalyze the following reaction anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD(+) =   2-(4-dimethylaminophenyl)diazenylbenzoate + 2 H(+) + 2 NADH.5. The FMN binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MQLLHIDSAITGDQSVSRQLTAQTVEAWKASHPATQVSYLDLVADAPAHFTMDAMAPRTGQTDGLSEAQQRENAVSERLVSQFLAADVVVIGAPFYNFAIPTQLKAWIDRIAQPGRTFQYTANGPEGLAKGKTVIVASSRGGVYSTSEGGRAMEHQESYLQTVLGFFGVTDVRFVRAEGVAMGGDAKAQALAAAAQAIEAHLAAAL",
    "response#2": "MKLLHLDSSILGDYSASRQLTASIVAKLQAADPALQYTYRDLAANPIGHLSGAHLAAAQNPPATRTPELANDLALGVQVLQEFLDADTVVIGVALYNFTISTQLKAWIDRVLVAGKTFRYTAEGALEGLAGNKRVILAVARGGRYGEGSPTAALEHAETYMRAALGFVGLHQPEVVVAEGLALGPEARAAGMAAAQAQIDELLSREQPAAAMRVLRVDASARVDDSVTRRLADLMLEILGERVPDLSVVRREAAGMPFVDDAWVGANSTDPEARDSAQRWALAKSDELVSEVMAADVLVIATPIYNFGVPASLKAWVDQVARARLTFRDTEYGPEGLLTGKKAYVLVASGGTEVGSDIDFATPWLEFVLGFLGITDVEVIAADRGMLRGDSARQNAAEHVFRLLDAYRARHGSGPL",
    "response#3": "MKLLHIDSSILGDNSASRQLSSQVTKAWQAAEPSAVVTYRDLAADAISHFSSTTLVAAGTTAELRNAAQQHEAELSATTLAEFITADAIVVAAPMYNFTVPTQLKAWIDRIAVAGQTFRYTEAGPEGLCGGKKVVIVSTAGGIHAGQASGVAHEDYLKLVFGFLGITDIEVVRAEGLAYGEEVRNNAMSAAQAKISEQLAKFLAHSQVSKVLYIKANIKLEGVSRTFQVSDHFIEEYKKQHPEDEVVVLDLYKENIDFLRPEDLEAMADINDETKKNTTFLKYAFQFAEADKYVIAAPMWNLGSPAILKAYFDYITITGITFHYTAEGPKGLLQNKKAVHIVARGGEYGNAPYEMGDRYVKTILGFLGVSDIQTIAIENLDIIGADVEAKVKEGKEKIYGKHNKTVLAIHGSPRGERSHSRRLADAFLQAWQAANPQGRLTRREVGRGALPHVSEAFIAAAFYPQPEARPLSMQADLALSDELVAELLDHQRLVISAPMHNFSVPSGIKAWVDHIVRLGLTFNTQQDNGVAQYQPLVQGKKVLIVTSRGGGGFGPGGEHEAMNHADRWLRTALGFIGIEDVTVVAAEGEESAPERFAQSCGEAQQRLRAIVSGA"
  },
  {
    "reference": "MRSSGYLNSNNGSSSSLESTNVNKSTDKNQLYLKVVCNCATDLVPQDTTGTGSESKKAYSSSKSINPVLVVQLNNTIKSTSKKLNTNQPSWNDELYMPLKNQDDSSLLIFSVWDKHTRYKNYLGELRLNVKDIFVDNGNFVDKTELKWYHLNSNTKYHSFVTGSILAGFELVIKNKKKRFHSSYNDKFQQLSIDSDVESAFKKWLSSLTKPTIYSVNNKINPNDQGFYDDELVNEFELESVLTERSKKSHLNVPGGNGLLSVPGDDCHDAGSFSDASFLSDGGYASDASANLFDQKETSSDSKKSRIKSKMRLRRSKQEYENFELASRQVLGVVFIEIVSCEDLPPYKNFTRTSFDMDPFVVVSFGKKTFRTGWKRHTLNPVFNERLAFELLPHESNFSIQFLVLDKDHFSFHDNVAHISLPLRTLTEYATAKPTEGDSSDVNTSDSTSFSQTGNGFTTEDTEFPENDSVQIIENETPSNKQSRKRYLSRKKVVGTQLDLKKFKAMSLSLQLHDQKYIGKYAPKLKIRVRFETYTELRKKFWRVLLEESNSTPENRDSCDYIELISLLDTLGCVNSDELVELFYSNLGKSSWGGDLLTFDEIIDQMETYVNQNVESGKVKIFEFEKCPICNEKRVSKKQDLDIITHFAICASKDWSVVSKLLSSSYVTPTQATKKWFTKALIKLTYGKYKLGGNSANILVQDRMTGIIVEEKMSVYVRLGIRLLYKGLDKARSKRVRILLKNMSIKQGKKFDAPQSKSDIASFIKFHK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. We require a protein that can specifically bind to the metabolites produced in the phosphatidylethanolamine from CDP-diacylglycerol: step 2/2 pathway.2. The protein design should be able to enhance the efficiency of phosphatidylethanolamine biosynthetic process, protein autoprocessing.3. The Charge relay system; for autoendoproteolytic cleavage activity of the protein must be engineered to enable effective catalysis of a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-   diacyl-sn-glycero-3-phosphoethanolamine + CO2. The designed protein sequence is ",
    "response#1": "LRVNVLKGRNLAAKDKRGTSDPYLVIALGDRKEATSVVSRTLNPEWNQVFEFPVTDTESALLEATCWDKDRFKKDYMGEFDVLLEDLFAA",
    "response#2": "MLKASVISGRNLAPKDRNGLSDPYLVVSLGEARQSTPTISKTLNPEWKVSFELPIVGVPLLECICWDKDRFGKDYMGEFDIPLEDIFSAGQIQPGR",
    "response#3": "LKASVISGRNLAPKDRNGLSDPYLVVSLGEARQSTPTISKTLNPEWKVSFELPIVGVPLLECICWDKDRFGKDYMGEFDIPLEDIFSAGQIQPKPQWYTN"
  },
  {
    "reference": "MAVPNGHAVHAPAIATRPLILTLGQIRVSIPVSTQANQWIAAEVLRDEFAHIQSQLDAVDSTAQLENEAEATVELSARFLAHVAANITKDPQSTSARTSLLLNVLKHFTSSYLATKDVHTLVSAFDTVTRKVVLAAYISAVAALQEQHISDIPSQPESALLSSQASGKASIFALFGGQGTNEVYFDELQNLYDIYSPFVASFIQTVTQDILIPLAEEEEESTFYTYGLDVLSWLSGATPRPSTLYLASVPVSFPLIGLTQLVQYLIVCRVANLTPGELRSRVTGATGHSQGIVSAVAIAASATFDEFVENSRKALKWLFYSGLRGQQAFPVTSLESDIVQDSIEGGEGTPSPMLSVTGLSLKELELHIAKTNKHLPANSQLFVSLHNGPKAFVVTGPSKALFGLVTSLRKVKASTGLDQSKVPFSQRKPVFSIRFLVVGVPYHSEYMSEATEKLIKEDLEDEELWQAKDLKIPVYNTEDGSDMRDLKTSITRSLCEQIFTSPIHWTKATAFPESATHALDFGPGGLSGIGPLTAKNFEGRGVRVIVAGEKGRGDVEIFGSLGIKYEEWWSKKWSPSLVRTSDGELHIDTPFSRLLGKPPIMVAGMTPSTVKAGIVSAVLNAGYHVELAGGGHYNPVALRSKVAEIQKQIPAGVGLTLNALYINPRQFTFQFPLWQEMRKEGLPIEGFCVAAGIPTTEKAVEIIEGLKAAGIKHVAFKPGSVDGIRQVINIATANPDFPIIMQWTGGRAGGHHSFEDFHQPILATYRTI",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Ideal protein should have a binding affinity for key enzymes in the fatty acid beta-oxidation, Lipid metabolism pathway to enhance the overall efficiency of the pathway.2. The designed protein should contain one or more Carrier domains that are essential for its function.3. The designed protein should be involved in fatty acid biosynthetic process, fatty acid beta-oxidation by localizing to fatty acid synthase complex and exhibiting 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity, 3-oxoacyl-[acyl-carrier-protein] synthase activity, fatty-acyl-CoA synthase activity, [acyl-carrier-protein] S-acetyltransferase activity. The designed protein sequence is ",
    "response#1": "VFSGMGSQWLTMATSMMALEPFARSIHQSAAVLKPLGIDLIELLTKNDPELWKSTVNPFVAITSMQVALYDVIKLLNIKIEGIIGHSFGEVACAYADGCLTLEQAILTSYWRGKIVEQSKLPRGVLAAIGLSWEETKKRCPPGIVPACHNGSDSVTISGLYEETKKFVEKLQAENVFARLVAGGEYPYHSPHMNAVAPDLIAALNKVIPEPKKRSSKWISTSFDKSNWFIEDACYASAKYFTNNLVSPVLFNEGMQEISKDCIAIEVAPHSLFDSIFKRCYQQHHYIGLMKRNFDMQGACAGFLYALETGANYIRSGRYKKVVVVSGDKMTSITDYQDRSTCPLFGDACGAVLLEPTTEEFGVLDTILRTDPKLFGGQGTNEVYFDELQQLFDIYGPYVRDFISTATYEILEPLAAKAQEEGHAYYSHGLDVLAWLTGTAPRPPLAYLSSVPVSFPLMGLTQLVQYLVSCRVLGLHPGEMRALIKGATGHSQGVVSAVCIAASSSYDSYKINVAKALRWLFACGLRGQEAFPTLTVEPSLVADCVQGGEGTPSPMLAVTGLELEKLRPHIEKTNKHLPENSQLIVSLNNGRKAFVVTGPPRALCGLVNNLRKVRAPQGLEQSKIPFSQYATVLHSKVNSDGFKSVGLFAPYWLRQKDLMVSTYEEAGVDPNDLVYFEAHGTGTNVGDPQEAKAIAEAYCKHREQPLLVGAVKSNLGHSEGSSGLNSVAKVIIAFENKCIPANLHG",
    "response#2": "FIIIAQADGLPQDDFNDIFTKDAAFWVYGNSKVLYANRISFLFDFQGPSLVIDTACSASLVALATAMNDIRMGVCDTAVVATGNLVPAPFGNSIYSCVGLLASDGKCKVWDKKADGFVRSETIGALILQRRSQAKRVYATVLHSKVNSDGFKSVGLFAPYWLRQKDLMVSTYEEAGVDPNDLVYFEAHGTGTNVGDPQEAKAIAEAYCKHREQPLLVGAVKSNLGHSEGSSGLNSVAKVIIAFENKCIPANLHFNEPKQEIYAIVNKQIEPVIKNTSFPAGIVGVNSFGVGGVNAHILLKSNPKPTRDLPTDIPRLFGGQGTNEVYFDELQQLFDIYGPYVRDFISTATYEILEPLAAKAQEEGHAYYSHGLDVLAWLTGTAPRPPLAYLSSVPVSFPLMGLTQLVQYLVSCRVLGLHPGEMRALIKGATGHSQGVVSAVCIAASSSYDSYKINVAKALRWLFACGLRGQEAFPTLTVEPSLVADCVQGGEGTPSPMLAVTGLELEKLRPHIEKTNKHLPENSQLIVSLNNGRKAFVVTGPPRALCGLVNNLRKVRAPQGLEQSKIPFSQL",
    "response#3": "FIIIAQADGLPQDDFNDIFTKDAAFWVYGNSKVLYANRISFLFDFQGPSLVIDTACSASLVALATAMNDIRMGVCDTAVVATGNLVPAPFGNSIYSCVGLLASDGKCKVWDKKADGFVRSETIGALILQRRSQAKRVYATVLHSKVNSDGFKSVGLFAPYWLRQKDLMVSTYEEAGVDPNDLVYFEAHGTGTNVGDPQEAKAIAEAYCKHREQPLLVGAVKSNLGHSEGSSGLNSVAKVIIAFENKCIPANLHFNEPKQEIYAIVNKQIEPVIKNTSFPAGIVGVNSFGVGGVNAHILLKANSKPPRHRHLPLFGGQGTNEVYFDELQQLFDIYGPYVRDFISTATYEILEPLAAKAQEEGHAYYSHGLDVLAWLTGTAPRPPLAYLSSVPVSFPLMGLTQLVQYLVSCRVLGLHPGEMRALIKGATGHSQGVVSAVCIAASSSYDSYKINVAKALRWLFACGLRGQEAFPTLTVEPSLVADCVQGGEGTPSPMLAVTGLELEKLRPHIEKTNKHLPENSQLIVSLNNGRKAFVVTGPPRALCGLVNNLRKVRAPQGLEQSKIPFSQIQSFPSSSNPRLVVFSGMGSQWLTMATSMMALEPFARSIHQSAAVLKPLGIDLIELLTKNDPELWKSTVNPFVAITSMQVALYDVIKLLNIKIEGIIGHSFGEVACAYADGCLTLEQAILTSYWRGKIVEQSKLPRGVLAAIGLSWEETKKRCPPGIVPACHNGSDSVTISGLYEETKKFVEKLQAENVFARLVAGGEYPYHSPHMNAVAPDLIAALNKVIPEPKKRSSKWISTSFDKSNWFIEDACYASAKYFTNNLVSPVLFNEGMQEISKDCIAIEVAPHSLFDSIFKRCYQQHHYIGLMKRN"
  },
  {
    "reference": "MLDNHTIAIVKSTIPLLAETGPKLTAHFYDRMFTHNPELKDIFNMSNQRNGDQREALFNAICAYATNIENLAALLPAVERIAQKHTSFNIQADQYQIVGNHLLATLDEMFSPGQEVLDAWGKAYGVLANVFIQREDDIYRSTETKTGGWSGVRPFRIVNKQLQSSVITSFTLEPTDGQPIADFQPGQYLAIYIKHDSFANQEIRQYSLTHAPNGKSYRIAVKREAQGTVSGYLHDTAREGDIVHLAAPHGDFFLDIPTDTPVALISGGVGQTPMLGMLHTLKQQDHQAKVLWLHAAENGTAHAFTDEIEQTGQALPHFDHHIWYREPQQTDRPGEDYHHSGLMQLASLKGELTTPDMHYYLCGPVVFMQFVAQQLLAMGIPAEQLHYECFGPHKVV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have a high affinity for FAD, NADP(+).2. A protein with oxygen binding, metal ion binding, heme binding, oxygen carrier activity is needed to enhance response to nitrosative stress in a specific context.3. A key design requirement for the protein is the optimization of the Charge relay system to achieve high catalytic efficiency for NADH + 2 nitric oxide + 2 O2 = H(+) + NAD(+) + 2 nitrate.4. The protein design should incorporate a binding pocket that specifically recognizes FAD to enhance enzymatic efficiency.5. The designed protein must have at least one FAD-binding FR-type domain.6. For general function, the protein need meet that Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds The designed protein sequence is ",
    "response#1": "MLSTNTIQIVKSTAPVLAVKGEEITTHFYKTLFTNHPELLNIFNHANQKKGRQQTALANTIYAAATYIDQLEVLLPAVKQIAHKHRSLAVKLEHYPIVGEHLLEAIKAVLGDAATDEIIEAWREAYGVIADVFISVEADFRSFRVTNKVKESEVITSFYLKPIDGGALPSFEPGQYVSVKVQPIGEEYTHIRQYSLSEAPGKDYFRLSVRREDAKDDQPQGIVSTYLHNHIEEGDQLQLSAPAGDFFLQTSDDRPVTLISGGVGITPMMSMLETLVAKDPAREVAFIHAAQNGRVHAFKKHVADLVAQHSNVRVYTVYDAPMEADRQGDDYHKEGYIASFLHGLISAGVGLTPMLAMLESLSEHRAPLTWVHATENGQQHAFKQHAKALVSKLEQGQTAIWYHQPSTEDRLGDDFHFTGLVNLDEIRDKLQAEHAQVYLCGPIGFMQYAKGQLRELGIDQARIHYEVFGPHAMLSENTIQIVKSTAPLLAKTGPQLTAHFYQRMFTHHPELKEIFNMSNQRNGDQREALFNAICAYANNIDNLPALLGAVEKIAHKHSSFLISAEQYQIVGSHLLATIDELFSPGEAVLDAWAEAYGVLANVFIAREA",
    "response#2": "MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFAHNPELKEIFNMSNQRNGDQREALFNAIAAYASNLENLPALLPAVEKIAQKHTSFQIQPEQYNIVGTHLLATLDEMFSPGQDVLDAWGKAYGVLANVFIHVADKVEESSLITSFYLKPKDGVKLPSYLPGQYITIKLDIPGEKNIVNRQYSLSAAPGHDYYRISVKRESDNKPEGKASNYLHDHVNKGDKVELSAPAGDFVADKVEESSLITSFYLKPKDGVKLPSYLPGQYITIKLDIPGEKNIVNRQYSLSAAPGHDYYRISVKRESDNKPEGKASNYLHDHVNKGDKVELSAPAGDFIGRDGVPLVLISGGVGVTPLMSMMKVSLDRYPDRRVVFLHAAVNGRVHAMRDDVAGLAEAHRNLTVHVCYDEPEPGDRFHTEGYIDVEFLRRIVPGPADYYLCGPIPFMRAVNRALKELGVSEERIHYEFFGPAGDLTAGPV",
    "response#3": "MLSENTIKIVKSTAPVLAERGVEITSYFYKTMFEAHPELLNIFNHANQKQGRQQTALANTVYAAATYIDQLEVLLPAVKQIAHKHRSLNVKKEHYPIVGEFLLKAIKDVLGDAATPEVLQAWGEAYQVIAQVFIGREADIYAKQEKTGWRTFVVREKRPESSVITSFVFEPADGGPVANFEPGQYISIAVNVPALGLQQIRQYSLSDMPNGRSYRISVKRESGGAYPPGYVSCLLHDHVNVGDEVRLAAPYGSFHINLTEDTPVTLISGGVGITPMMSMLETLAKEGTRPFRIVAKTPRSALITSFEFEPVDGGAVADYQPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYRIAVKREDGGQVSNWLHNHANVGDVVHLAAPAGDFFMDAPIYLISGGVGITPMMSMLKRVLTQQEHPVSFYHGARNSAVHAMKERVQALAEQHPNLELHVCYSEPDDGDVAGHFDKEGYMTAEILTQHVPAPNDFYVCGPVPFMRMQHDALKALGVPESHIHYEFFGPAGTLEET"
  },
  {
    "reference": "MGSFNTSFEDGFILVGFSDWPQLEPILFVFIFIFYSLTLFGNTIIIALSWLDLRLHTPMYFFLSHLSLLDLCFTTSTVPQLLINLCGVDRTITRGGCVAQLFIYLALGSTECVLLVVMAFDRYAAVCRPLHYMAIMHPHLCQTLAIASWGAGFVNSLIQTGLAMAMPLCGHRLNHFFCEMPVFLKLACADTEGTEAKMFVARVIVVAVPAALILGSYVHIAHAVLRVKSTAGRRKAFGTCGSHLLVVFLFYGSAIYTYLQSIHNYSEREGKFVALFYTIITPILNPLIYTLRNKDVKGALWKVLWRGRDSG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a Helical; Name=2, Helical; Name=3, Helical; Name=1, Helical; Name=7, Helical; Name=6, Helical; Name=5, Helical; Name=4 domain that is long enough to span the entire membrane.2. For general function, the protein need meet that Odorant receptor.3. The designed protein should contain Cytoplasmic, Extracellular topological domains.4. The designed protein must be able to perform G protein-coupled receptor activity, olfactory receptor activity under specific conditions. The designed protein sequence is ",
    "response#1": "GNLGLIVLIWNDPHLHMPMYLFLGGLAFSDACTSTSITPRMLVNFLDKTAMISLAECITQFYFFASSATTECFLLVMMAYDRYVAICNPLLYPVMMSNKLSAQLLSISYVIGFLHPLVHVSLLLRLTFCRFNIIHYFYCEILQLFKISCNGPSINALMIFIFGAFIQIPTLMTIIISYTRVLFDILKKKSEKGRSKAFSTCGAHLLSVSLYYGTLIFMYVRPASGLAEDQDKVYSLFYTIIIPLLNPFISALNYGLKTVEMKSTFRRIFGK",
    "response#2": "GNLGLIVLIWNDPHLHMPMYLFLGGLAFSDACTSTSITPRMLVNFLDKTAMISLAECITQFYFFASSATTECFLLVMMAYDRYVAICNPLLYPVMMSNKLSAQLLSISYVIGFLHPLVHVSLLLRLTFCRFNIIHYFYCEILQLFKISCNGPSINALMIFIFGAFIQIPTLMTIIISYTRVLFDILKKKSEKGRSKAFSTCGAHLLSVSLYYGTLIFMYVRPASGLAEDQDKVYSLFYTIIIPLLNPFIYSLRNKELRNAFKKLIRR",
    "response#3": "GNVLIVGVVRADTRLQTPMYFFLGNLSCLEILLTSVIIPKMLSNFLSRQHTISFAACITQFYFYFFLGASEFLLLAVMSADRYLAICHPLRYPLLMSGAVCFRVALACWVGGLVPVLGPTVAVALLPFCKQGAVVQHFFCDSGPLLRLACTNTKKLEETDFVLASLVIVSSLLITAVSYGLIVLAVLSIPSASGRQKAFSTCTSHLIVVTLFYGSAIFLYVRPSQSGSVDTNWAVTVITTFVTPLLNPFIYTLRNTEVKNALKRIVRR"
  },
  {
    "reference": "MLKNKKIILGVCGSIAAYKSAILVRLLVKAGANVKVILTAGAANFITPLTLATLSKNPVYTQYFEEETGVWSNHVELGLWADFMVIAPASANTLAKMASGLCDNLLTAVYLSAKCPVYVAPAMDLDMWKHESTAGNVQKLLTYGNQVIQPGSGELASGLFGEGRMAEPEDILAFLTDKVRSSLPLLGKKIMVTAGPTYEAIDPVRFIGNHSSGKMGFAIANRFASLGADVTLITGPTTEKSNYELKRIDVVSAADMLSACKAEFGKADITVMSAAVADYTPVTRAEQKIKKTSNEFNLELKKTTDILATLGQLKTDRQILVGFALETQNEETYAKEKLIKKNLDLIVLNSLNDKGAGFKMDTNKITIFNKAAEKIVFEMKSKTEVAKDICNEILKLVNV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. A protein with phosphopantothenate--cysteine ligase activity, metal ion binding, FMN binding, phosphopantothenoylcysteine decarboxylase activity functions is required to enable pantothenate catabolic process, coenzyme A biosynthetic process.3. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A.4. The designed protein should interact with enzymes and substrates within the Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis pathway to enhance or regulate its activity.5. The protein should have a high affinity for CTP.6. The desired protein should be able to perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO27. The designed protein should contain one or more DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALALAAAVLGRSGTLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHVVGADGAVREVPAGSKDVVADAVLDAVVRLLD",
    "response#2": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALAIASTVLGDRLAGRHVVVSAGGTREPLDPVRFLGNRSSGRQGYALARTAVARGARVTLVAANTALPDPAGADVVRVGTAVQLREAVLKAAADADAVVMAAAVADFRPARYAQGKIKKRDGQEPEPVELVRNPDVLAELSSTRPRPGQVVVGFAAETDDVLVNGRAKLARKGCDLLVVNEVGEEKTFGSTENEAVVLGADGSTAAIPHGPKSALADVVWDLVAPRLTPG",
    "response#3": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALALASRLLAAPRDLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHVLDAGGHVDRVVLGVSGGIAAYKACEVLRRLTESGHDVRVVPTASALRFVGEPTWAALSGHPVATGVWEDVQEVPHVRIGQEADLVVVAPATADILARAAHGLADDLLTNTLLTARCPVVFAPAMHTEMWEHPATVENVATLRRRGAVVVEPAVGRLTGKDTGKGRLPEPGEIFEVCDAVAGVLAAARAAGR"
  },
  {
    "reference": "MSDASATTDQAINKSAANKTDAAEKAGSKLRHHVRIVTAASLFDGHDAAINVMRRIMQASGAEVIHLGHNRSVAEIVHCAIQEDVQGIAITSYQGGHVEYFKYMIDLLREAGANIKVFGGGGGTILPSEIAELHEYGVARIYSPDDGRAMGLQGMIDDVLRQCDFEKRPADFAPLVEQLKKREPSTIAGLITIAENFPDSGEGLRKALAPLTATDLKVPVLGITGTGGAGKSSLVDELVRRFLSDFDDKTIAVLSVDPSKRKTGGALLGDRIRMNSIDDPRVYMRSLATRQSNLALSKHVRDSIEICRAAGFDLIIVETSGIGQSDTEITDHADVSLYLMTAEYGAATQLEKIDMLDFADVIAINKFDKRGSLDALRDVKKQWKRNHNAFATTDEDVPVYGTIASQFNDPGMNRLYKTIVDTVAKRTNSPLHSKHEVTPGMSEKRWIIPPEKTRYLAEIVDTCESYDELARQQAAIARRMYQLHGTIEALRANVGKKTLEIVEPKGKDDVVQVTQKVEGEPAFLGELVELYNDLEARLAPECKKLLAEWPATKRRYAAAKYQFQVRDKVIEIDLFSESLSHLRIPKIALPKYEDWGDILTWLLREGPPGQFPFTSGVFPLKRENEDPARMFAGEGGPERTNKRFHYVSRGLPAKRLSTAFDSVTLYGEDPDHRPDIYGKVGNSGVSIANVDDAKKLYSGFDLSDPATSVSMTINGPAPMLLGFFLNAAIDQGCEKWIRQNGKVDEVEKKIAEIFAKKGLPRPTYQGTL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The substrate, GTP, adenosylcob(III)alamin binding site should be stable and able to withstand changes in environmental conditions.2. The protein must exhibit pivalyl-CoA mutase activity. The designed protein sequence is ",
    "response#1": "SRAVSQYPNHELNFITSSAHDSIHTKTFTEFDQYVRALAQAMLAWGKPAGSVIVVYLTEHEDNMAAIWASLFAGYVPCLQPALSAQQAHKEGHVAHIKNLFSSATWLTNESGAEQVQSIPGLDIHLLSELQASAETVSVDFQTHQPHLDDEAILFLTSGSTGFSKAVVHTHRTILAGCNAKGQSYGLTSESKIMNWVGFDHVVGSLGMHITPLLCGASQLHVHASAILSDSLRFLHLIEEKSIQLVFAPNFLLAKLTRDLEKRSDLFGKFDLSSIKRINSGGEAVVSSTAQAFARTLKNLAKDGDASFVFSTGFGMTETGAGCIYDTIDVLGTSPPHEFLEIGTPVAGCEMRIVNPEDGVTPRPDGESGELQVRGPMVFVRYY",
    "response#2": "SRAVSQYPNHELNFITSSAHDSIHTKTFTEFDQYVRALAQAMLAWGKPAGSVIVVYLTEHEDNMAAIWASLFAGYVPCLQPALSAQQAHKEGHVAHIKNLFSSATWLTNESGAEQVQSIPGLDIHLLSELQASAETVSVDFQTHQPHLDDEAILFLTSGSTGFSKAVVHTHRTILAGCNAKGQSYGLTSESKIMNWVGFDHVVGSLGMHITPLLCGASQLHVHASAILSDSLRFLHLIEEKSIQLVFAPNFLLAKLTRDLEKRSDLFGKFDLSSIKRINSGGEAVVSSTAQAFARTLKNLAKDGDASFVFSTGFGMTETGAGCIYDTIDVLGTSPPHEFLEIGTPVAGCEMRIVNPEDGVTPRPDGESGELQVRGPMVFVRYY",
    "response#3": "SRAVSQYPNHELNFITSSAHDSIHTKTFTEFDQYVRALAQAMLAWGKPAGSVIVVYLTEHEDNMAAIWASLFAGYVPCLQPALSAQQAHKEGHVAHIKNLFSSATWLTNESGAEQVQSIPGLDIHLLSELQASAETVSVDFQTHQPHLDDEAILFLTSGSTGFSKAVVHTHRTILAGCNAKGQSYGLTSESKIMNWVGFDHVVGSLGMHITPLLCGASQLHVHASAILSDSLRFLHLIEEKSIQLVFAPNFLLAKLTRDLEKRSDLFGKFDLSSIKRINSGGEAVVSSTAQAFARTLKNLAKDGDASFVFSTGFGMTETGAGCIYDTIDVLGTSPPHEFLEIGTPVAGCEMRIVNPEDGVTPRPDGESGELQVRGPMVFVRYYQ"
  },
  {
    "reference": "MLDSKLVRTQLTEIAERLATRGFALDVARFEALESQRKSVQVRTEQLQAERNSRSKSIGQAKARGEDIAPLLAEVDQMGSDLEAGKRELDAIQNELDNLLLNIPNLPHESVPVGADEDGNVEVARWGTPRSFDFEIKDHVALGEQHGWLDFETAAKLSGARFALLRGPIARLHRALAQFMINLHTGEHGYEEAYTPYLVQAPALQGTGQLPKFEEDLFKIRREDQADLYLIPTAEVSLTNIVAGEILDAKQLPLKFVAHTPCFRSEAGASGRDTRGMIRQHQFDKVEMVQIVEPSKSFEALEGMTAHAERVLQLLELPYRKLALCTGDMGFSAVKTYDLEVWVPSQDKYREISSCSNCGDFQARRMQARYRNPETGKPELVHTLNGSGLAVGRTLVAVLENYQQADGSIRVPEVLKPYMGGIEVIG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be optimized for ATP binding. The designed protein sequence is ",
    "response#1": "GPGELILYPRRRKESHKGENGIVAIIGGGAYTGAPYLAGMAALRAGCDLVYVCCPSSVWSVVASFSPDLIVRKISGEFLTVEGLKEVEDIIEKADAIVVGPGLGKRKEVLEACKFIFENYDKKFVYDADAIEVLKGKKCNQKVIITPHSGEFKRLTGIDLPDDIEERSRIVKKEAKKLEATILAKGAIDIISDGKRLKMNKIHNEGMTVGGTGDTLAGICAAMLAKKIAPFYSACISAFINGMAGNMAYEEKGNIVATDMIEKIPLVIS",
    "response#2": "VGPGELILYPRRRKESHKGENGIVAIIGGGAYTGAPYLAGMAALRAGCDLVYVCCPSSVWSVVASFSPDLIVRKISGEFLTVEGLKEVEDIIEKADAIVVGPGLGKRKEVLEACKFIFENYDKKFVYDADAIEVLKGKKCNQKVIITPHSGEFKRLTGIDLPDDIEERSRIVKKEAKKLEATILAKGAIDIISDGKRLKMNKIHNEGMTVGGTGDTLAGICAAMLAKKIAPFYSACISAFINGMAGNMAYEEKGNIVATDMIEKIPLVIKKFD",
    "response#3": "VGPGELILYPRRRKESHKGENGIVAIIGGGAYTGAPYLAGMAALRAGCDLVYVCCPSSVWSVVASFSPDLIVRKISGEFLTVEGLKEVEDIIEKADAIVVGPGLGKRKEVLEACKFIFENYDKKFVYDADAIEVLKGKKCNQKVIITPHSGEFKRLTGIDLPDDIEERSRIVKKEAKKLEATILAKGAIDIISDGKRLKMNKIHNEGMTVGGTGDTLAGICAAMLAKKIAPFYSACISAFINGMAGNMAYEEKGNIVATDMIEKIPLVIKKFY"
  },
  {
    "reference": "MDVKYDLNSYVRVLKLASTPSWQEFSQISKIAGAGIFLVGLLGFIIFAVMSFLPGGV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The protein design should be able to enhance the efficiency of protein secretion.3. For general function, the protein need meet that May contact the channel plug during translocation. The designed protein sequence is ",
    "response#1": "GNISIITLIRISSQLHTPMYLFLSHLAFVDILYSTSVSVIMHMELLGHGLALPVAACAAQLCITVSFGSAECFLLAAMAYDRYVAICSPLLYSTLMSPRVCFLLLGMSYVGGCMNGWTFTGCLLSLSFCGPNQIDHFFCDFSPLLKLSCSDVSIIGIIPSISSGSIIVVTVFVIAVSYIYILITILNMRSTEGRHKAFSTCTSHLTAVTLYYGTITFIYVMPKSNYSTEQNKVLSVFYTVVIPMLNPLISAVEY",
    "response#2": "GNISIITLIRISSQLHTPMYLFLSHLAFVDILYSTSVSVIMHMELLGHGLALPVAACAAQLCITVSFGSAECFLLAAMAYDRYVAICSPLLYSTLMSPRVCFLLLGMSYVGGCMNGWTFTGCLLSLSFCGPNQIDHFFCDFSPLLKLSCSDVSIIGIIPSISSGSIIVVTVFVIAVSYIYILITILNMRSTEGRHKAFSTCTSHLTAVTLYYGTITFIYVMPKSNYSTEQNKVLSVFYTVVIPMLNPLISALNY",
    "response#3": "GNISIITLIRISSQLHTPMYLFLSHLAFVDILYSTSVSVIMHMELLGHGLALPVAACAAQLCITVSFGSAECFLLAAMAYDRYVAICSPLLYSTLMSPRVCFLLLGMSYVGGCMNGWTFTGCLLSLSFCGPNQIDHFFCDFSPLLKLSCSDVSIIGIIPSISSGSIIVVTVFVIAVSYIYILITILNMRSTEGRHKAFSTCTSHLTAVTLYYGTITFIYVMPKSNYSTEQNKVLSVFYTVVIPMLNPLISAAYAL"
  },
  {
    "reference": "MKEIKGTIASPKGFLADAVHAQLKYKNLDLGLILSQVPAAIAGVFTTNKVCAAPVLIDRQIVKKGQARAIICNSAVANAVTGEQGYANALKTQKLLAEKFELKAEEVAVCSTGVIGVQLPMEKIATGISKLSQNEGTAAYFAKAILTTDTQTKTINFEAEIGGQIVNMAGVCKGSGMIHPNMATMLAFITTDAKIAQALLQKTLSEIIETTFNQITVDGDTSTNDTVLLMANGQAKNNEILEGSSDYLLFKEMLAKVCQSLAKQIAADGEGATKLIEVTVKGAPNDLTARFIAKKIVGSSLVKTAIFGADPNWGRIISSIGQVANFEVSDIELKLQDELVLYHSTPVDFDAAFLSEKLKEDKIEIIADLNAGSGLGQAWGCDLTYKYVEINALYTS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The substrate binding site should be located in a region of the protein that is accessible to the ligand.2. For general function, the protein need meet that Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.3. We require a protein that can specifically bind to the metabolites produced in the N(2)-acetyl- L-ornithine from L-glutamate: step 1/4, L-arginine biosynthesis, Amino-acid biosynthesis pathway.4. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile to enable effective catalysis of acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate.5. A protein with glutamate N-acetyltransferase activity, acetyl-CoA:L-glutamate N-acetyltransferase activity, methione N-acyltransferase activity functions is required to enable arginine biosynthetic process. The designed protein sequence is ",
    "response#1": "MSVLSGAXRPNRAXSXGXRXPGXHXGAXRXAGXTXXGXEPAXLAXDXTQXVX",
    "response#2": "MNYENIVEALKQIKEGKMIIVVDDEDRENEGDFVCAAEKITPEIINFMATYGKGLICMPMSQERADALDLHLMVGHNTDNHETAFTVSIDHVSTTTGISAHERSETIQKVLDDNANAKDFVRPGHIFPLRYREGGVLVRAGHTEACVDLARLAGLYPAGVICEIMNEDGTMARVPELMEFAKKHNLKIITIADLIEYRRRNEKLVQM",
    "response#3": "MNLYKNSVLFKKYQKLSEKTNNLKIYTDTKKIDKKSLFVAIKGENFDAHAFLKKIYKNKIKYLISEKMLTNDKFNFIKVKNSRKYLGFLSKNFYLQKKINIIA"
  },
  {
    "reference": "MRESIVERRTSETDIVLKIGLDRTEPHQITISTGIPFFDHMVQAMSRHGGFDLNITAKGDLEVDSHHTIEDIGIVLGTALQQALGDGRGIKRFGYAIIPMDEALAEAVLDCGGRGYLVFKGTFGNSTVGGIDTSLFEHFFYSLCIHAGITAHIRFSGQNDHHTAEAIFKAFGIALGQAVTIIPESNQIPSTKGTL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have imidazoleglycerol-phosphate dehydratase activity and be active in cytoplasm.2. The designed protein should interact with enzymes and substrates within the Amino-acid biosynthesis, L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9 pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MPQTP",
    "response#2": "MNPLINK",
    "response#3": "MPIGVPKVPFRNPGEEDASCWVDLYR"
  },
  {
    "reference": "MNTDTALLTVSQMSESDSLTVAAGTSAIVLMENAGRAVAQEIIRRSRPCRVSVLCGPGNNGGDGFVAARHLAEAGWPVRVALLGTREHLTGEARYHAEQWLESIEPMNAAVLDKAELVVDAIFGAGLSRALEGAAADTLAAAASLKIPIVAIDVPSGLMGDTGEVLGSMAAVFTVTFFRKKPGHLLLPGRMLCGEVVVADIGIAASTLEVINPNTFENDPALWLAEMPRLGDGGNKYTRGHALICGGYPVTGASRMAARAASRAGAGLTTIAVPEIAFPIYAGALTSIMVHPLVKPEDVDLLLEDHRFTAFLVGPGCGAGLLTQDHARKMLATGLPTVIDADAITAFQDEPTQLDAAIVGPCVLTPHDGEFRRLFDTAGDKLTRTRAAAHRSGAIIVLKGSDTVITAPDGRSIINTNAPPTLATAGAGDVLSGIVLGLLAQGMDPFLAAAAAVWMHGAAATEFGPGLIAEDLADLLPGVLCRLNF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. The protein should have a high affinity for K(+), (6S)-NADPHX, AMP.3. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.4. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.5. The designed protein should be optimized for ATP binding, metal ion binding, NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity activity in order to improve nicotinamide nucleotide metabolic process.6. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. The designed protein sequence is ",
    "response#1": "MQEMDRKTIQELGIPGIVLMENAGRGAAHTFLSHFSPEQGARVIIICGRGNNGGDGYVVGRYLHEAGLRPTIMVLSEKEKISGDARMNLDIIHGMGLDIRYLPNEQSWQDQKKELTHCNYLVDAILGTGLNSPVRGYYGAVIDEINSSGKPVMAIDIPSGLNADNGAIMGLAVRADLTVTFGLPKLGQLVFPGCDMVGRLVRIDICIPHSVAPDSYKNTHGHALLIAGSRGLAGAAVLCANAAMSSGAGLVTVATPSSAQSAVAAQAMPEVMTAALPETTNGAVSFDAVERVLELRERASVIAIGPGLTSADETTRRFVREIIERRSTPVIIDADGLNALAPWPDDLHGTEEHPLILTPHPGEMLRLLGTEDHAVLSDRVTAAREFATAHSLILVLKGTRTLVAAPDGRVFINPTGNAGLGTAGAGDTLTGIITGLNAQSYATLKEEADALSATIAAVYVGGLAGDMAARESGMRTMTASDIDESSLAIRLPKRARTAHKGSSGFVTLAGGDEGYTGAILMATRSALRAGAGMVTVASRGAHAAVLAAAQPEAMFRPTESADDFVARIDKTKVVAIGPGLGQGDWGRALWRAALRSDKPLVVDADALNLLAADPQSCDRRAAPWILTPHPGEAARLLGCTVAEIEADRVAAVREVAHRYGGTVVLKGAGTLVCGQQLAVCPYGNPGMAVGGMGDALTGIIAAFVAQGLAPEPAAQTGVMVHALAGDVAAKAGERGLLPSDLIECLRQIVNPKPF",
    "response#2": "MQMAEFNSEYLGVSHFQLMEAVGANISREIATRTATKPNPRIHIISGPGKNGGDGFAVARHLASQGLKVRVTLVGRPNDVKDEAAKQQLNTIIQMNDSIQFESLSDSSQLRPVEADIILDAILGYGVQGDLRQPLLGAVRIINRSRGYKIALDLPSGLDSDTGEPHGEAVKADLTISLHKIKQGLVKGREFAGETLSLSIGIPPEAERLVLRRREKSSHKGDNGRVLIVGGGPFAGAPALSALASLRTGADWVTVAAPRNVASIIASISPNLIVQPLSGSGSGSILTEDDVSLVSSLMQMHDVLVIGMGLGAAEETKRAVKLIIEDEAVKKVVVDADGLYGLDLPLKVSKLKSKLKSKQVIITPHAGEFSKMDAQRPPPDGEERLGFIQEFSLRNRVVTLLKAAHDVISDGKRIKVNATGNAGMTVGGTGDVLSGITGALFATSEDAFEVATAAAFISGSAGDAAFRDKGLSLLATDVVEAIPGII",
    "response#3": "MQEMDRKTIQELGIPGIVLMENAGRGAAHTFLSHFSPEQGARVIIICGRGNNGGDGYVVGRYLHEAGLRPTIMVLSEKEKISGDARMNLDIIHGMGLDIRYLPNEQSWQDQKKELTHCNYLVDAILGTGLNSPVRGYYGAVIDEINSSGKPVMAIDIPSGLNADNGAIMGLAVRADLTVTFGLPKLGQLVFPGCDMVGRLVRIDICIPHSVAKSSHKGDNGRVLIVGGGPFAGAPALSALASLRTGADWVTVAAPRNVASIIASISPNLIVQPLSGSGSGSILTEDDVSLVSSLMQMHDVLVIGMGLGAAEETKRAVKLIIEDEAVKKVVVDADGLYGLDLPLKVSKLKSKLKSKQVIITPHAGEFSKMDAQRPPPDGEERLGFIQEFSLRNRVVTLLKAAHDVISDGKRIKVNATGNAGMTVGGTGDVLSGITGALFATSEDAFEVATAAAFISGSAGDAAFRDKGLSLLATDVVEAINANKGTFGKVLTVAGSVNYSGAAYLACSGAMRAGAGLVTLAVPVNLQPVLAGKLNEVTYLPLSESVKGVVSGEASFIVLSSLHEYTTLLIGCGLGQHDMTVSFTKGILFGNKPIMPLVIDADALNVLAKTNDWWQKLMMDAILTPHPGEMSRLTGIKIEEIQSNRIDTAMLFAQKWGKTVVLKGAYTVIASADGRCRVSPFANPGMASAGTGDVLAGIIAGLTAQGLDNFNAACLGVWLHGQAGEIVKAELGDTGMLNANKGTFGKVLTVAGSVNYSGAAYLACSGAMRAGAGLVTLAVPVNLQPVLAGKLNEVTYLPLSESVKGVVSGEASFIVLSSLHEYTTLLIGCGLGQHDMTVSFTKGILFGNKPIMPLVIDADALNVLAKTNDWWQKLMMDAILTPHPGEMSRLTGIKIEEIQSNRIDTAMLFAQKWGKTVVLKGAYTVIASADGRCRVSPFANPGMASAGTGDVLAGIIAGLTAQGLDNFNAACLGVWLHGQAGEIVKAELGDTGML"
  },
  {
    "reference": "MSEMESDILRAGWEAWRRNPVTAVPFVLHGLSVIAVSIATLLSAIYAIMPELPAAILSGDFGDEAFKQMFGEFLKRCLGNIELLGVTFVFGLVIVAALAALFKAWWIKLCYDALEGKAILSTAFHHAKSLFVPLFKFELILTLLIGIAFIPLINLGLDFFRNIHMLTKEAALYYVISFMIWSAFLGIFAITIQFLFTFVPYAIVIDLKGTLSGIRRGVMVLRHNLVDTIIMWLLVGLAGMALQVAAYPFRFFGFWGTLAGTLVAVILGWVAVMPITTCWWVELYRRRSKTLNSFPNG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "MF",
    "response#2": "MFEKK",
    "response#3": "LHHHHHH"
  },
  {
    "reference": "MKEDAGNPLHLTSLNHVSVLCRSVDESMNFYQKVLGFIPIRRPESLNFEGAWLFGHGIGIHLLCAPEPEKLPKKTAINPKDNHISFQCESMGVVEKKLEEMGIDYVRALVEEGGIQVDQLFFHDPDGFMIEICNCDSLPVVPLVGEMARSCSRVKLHQMVQPQPQTQIHQVVYP",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should contain one or more VOC domains that are essential for its function.2. The Proton donor/acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The protein should be designed to localize specifically to plasma membrane, cytoplasm in order to perform its role in response to salt, response to methylglyoxal, cellular detoxification of methylglyoxal, regulation of jasmonic acid mediated signaling pathway more effectively.4. The protein must exhibit the following characteristics: Involved in the detoxification and scavenging of methylglyoxal (MG), a cytotoxic aldehyde produced in response to primary metabolism alteration observed during biotic and abiotic stresses. The designed protein sequence is ",
    "response#1": "SLIQWTLQHKASLTPHTLYATGTTGHLLARESGLNIQALLSGPMGGDQQLGGLIAEKKIDILIFFWDPMNAAPHDPDVKALMRIATVWNIPVAV",
    "response#2": "SLIQWTLQHKASLTPHTLYATGTTGHLLARESGLNIQALLSGPMGGDQQLGGLIAEKKIDILIFFWDPMNAAPHDPDVKALMRIATVWNIPVAD",
    "response#3": "SLIQWTLQHKASLTPHTLYATGTTGHLLARESGLNIQALLSGPMGGDQQLGGLIAEKKIDILIFFWDPMNAAPHDPDVKALMRIATVWNIPVAV"
  },
  {
    "reference": "MNKLDISTINIEQLTSDHAKKIVDFLALELQKYDQAYYSDNNPLVTDAQYDVLKNLNNKIVAKFPHLALVNDHSKKVGFTPSAQFSKVVHLKPMLSLANGFCVEDISNFITKIQNFLKIDHCPKIVCEYKIDGLSFNARYEYGVLTLASTRGDGQIGENITENLKTIQSFPQTLPITDKVFEVRGEIYITNNDFRVLNIQQQKLGKALFSNPRNAAAGSIRQLDPAITAQRPLKYFVYALGEVTNHHFASTQFELLQKLSQLKFSVNTDYILSDNLHSMIEFYNNVATERNNLAFEIDGVVYKVNDFALQERLGATSTSPRFAIAYKFPALIGRTKITNITLQVGKTGAVTPVALLIPINIAGVTISRATLHNKQEIESKDIRIGDYVFLHRAGDVIPKINGVDLSARDAQSSTRFIFPATCPSCNQNLVVNEGETVARCGNSLACPAQIYERICHFVSKDALNIDGLGRQRIRFLLDNKYIVNIVDIFLLEENNKLLSSNKLEDIAGWGIKSVNKLFKNINQAKNVILDRFIYALAVKHVGKYSAKLLAKEFKTAKNFIDQSLKLANNVTEIYEKLCNIEGLGVKTADQLKQFFMVSANVNLITKLVNILTIQDWQYHGDNLLLSNQTIVFTGTFATVSRSEIKVQAEKLGAKVGTQVSNNTDLLVVGNKAGNKLQKAQQLGIKIINEEEWIKMVNE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for NAD(+) binding.2. For general function, the protein need meet that DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double- stranded DNA using NAD as a coenzyme and as the energy source for the reaction3. The protein must be engineered to have a cooperative binding mechanism with Mn(2+), Mg(2+) to ensure proper regulation.4. The protein's N6-AMP-lysine intermediate must be carefully engineered to ensure that the amino acids involved in NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-   (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-   nicotinamide D-nucleotide. are properly positioned for optimal catalysis.5. A protein with DNA binding, metal ion binding is needed to enhance DNA replication in a specific context. The designed protein sequence is ",
    "response#1": "MKKEEYLEKVALANLWMRAYYEKDEPLASDEEYDALIRELRVFEEQNKDEISKDSPTQKIAPTIQSEFKKIAHLKRMWSMEDVFDESELRAWAKRAKCEKNFFIEPKFDGASLNLLYENGKLVSGATRGDGEVGEDITLNVFEIENIPKNIAYKERIEIRGEVVILKDDFEKINEKRALLNQSLFANPRNAASGSLRQLDTSITKERNLKFYPWGVGENTLNFTKHSEVMQFIRELGFLKDDFVRLCANLDEVLKAYDELLALREKKPMMMDGMVVRVDDLALCEELGYTVKFPKFMAAFKFPALEKTTRLIGVNLQVGRSGVITPVAVLEPVNLDGVVVKSATLHNFDEIARLDVKINDFVSVIRSGDVIPKITKVFKERREGLEMEISRPKLCPTCQSELLDEGTLIKCQNIDCEDRLVNSIIHFVSKKCMNIDGLGTKIIQQLFDQNLIKDVADLYYLTKRKALDIDGLGEKVVSQLVEAIADSKNRPLHKFIFSLGIKNIGEKTAYMLAQQFKSFANWFDKMSMLKDDTQTEDEIRNLDGMGSCICESLLDFFSDSDNCNMIDELVSLGVTTTAEKAKLSGKKFVFTGTLLSITREEAKEIIKKAGGIVVNSISKQIDYVVAGEKAGSKLAKANELGIAIIEEEEFYQLLK",
    "response#2": "MKKEEYLEKVALANLWMRAYYEKDEPLASDEEYDALIRELRVFEEQNKDEISKDSPTQKIAPTIQSEFKKIAHLKRMWSMEDVFDESELRAWAKRAKCEKNFFIEPKFDGASLNLLYENGKLVSGATRGDGEVGEDITLNVFEIENIPKNIAYKERIEIRGEVVILKDDFEKINEKRALLNQSLFANPRNAASGSLRQLDTSITKERNLKFYPWGVGENTLNFTKHSEVMQFIRELGFLKDDFVRLCANLDEVLKAYDELLALREKKPMMMDGMVVRVDDLALCEELGYTVKFPKFMAAFKFPALEKTTRLIGVNLQVGRSGVITPVAVLEPVNLDGVVVKSATLHNFDEIARLDVKINDFVSVIRSGDVIPKITKVFKERREGLEMEISRPKLCPTCQSELLDEGTLIKCQNIDCEDRLVNSIIHFVSKKAMNITGFGPSIVEKLFDLKLIKDVADIYKLDIQTLASLPRMGEKSAKNLLSAIQSSKSNSAEKFIFSLGIKNIGEKTAYMLAQQFKSFANWFDKMSMLKDDTQTEDEIRNLDGMGSCICESLLDFFSDSDNCNMIEQLLQGKTFVLTGTLPSLTRQQASEMIESRGGKVSSSVSKKTSYVVAGDDPGSKLDKALQLELTILDEAGFLNL",
    "response#3": "MKKEEYLEKVALANLWMRAYYEKDEPLASDEEYDALIRELRVFEEQNKDEISKDSPTQKIAPTIQSEFKKIAHLKRMWSMEDVFDESELRAWAKRAKCEKNFFIEPKFDGASLNLLYENGKLVSGATRGDGEVGEDITLNVFEIENIPKNIAYKERIEIRGEVVILKDDFEKINEKRALLNQSLFANPRNAASGSLRQLDTSITKERNLKFYPWGVGENTLNFTKHSEVMQFIRELGFLKDDFVRLCANLDEVLKAYDELLALREKKPMMMDGMVVRVDDLALCEELGYTVKFPKFMAAFKFPALEKTTRLIGVNLQVGRSGVITPVAVLEPVNLDGVVVKSATLHNFDEIARLDVKINDFVSVIRSGDVIPKITKVFKERREGLEMEISRPKLCPTCQSELLDEGTLIKCQNIDCEDRLVNSIIHFVSKKAMNIAGFSEKTAEQLFNKLEIKSIADLYKIKKGKLTGLDRMGPKSAQNLIASIKKSKTTSLNSRFLYALGIREIGEAGARVLAEHYCDVEGLKAATIEELMTLNDIGPVAASHVVHFFAQAHNLEVIQGVLAGKTVVLTGTLPTLTREAAKEMLEAAGAKVAGSVSKKTDYVVAGADAGSKLAKAEELGIPVLDEAGMHTLLEGA"
  },
  {
    "reference": "MSSRYAIVLAAGQGTRMKSKLYKVLHQVCGRAMVDHVITQVELANPEQIVTVVGHGAEAVEELLGDRSAYAFQEAQLGTGHAVLQAEAQLGTKEGMTLVVNGDTPLLTTQTFEKLFAYHQAKGAKATILTANADDPTGYGRIIRNDLGIVERIVEQKDASKEEAAVHEINTGVFCFDNQTLFNALHQVTNQNAQGEYYLTDVIQIIKDAGGIVAAYQTPDFTESLGVNDRVALSVATEIMQQRINEQHMRNGVTLIDPKTAHIDIDVKIGADTLVEGDVNLRGHTEIGADCVIGSHSELVDAKIHDRVHITSSTIEEAEMMTGSNIGPNSHLRPKAIIGEDAHIGNFCEVKNATIGARTKVGHLSYVGDATLGTDINVGCGVVFVNYDGVKKFHTNVGDHSFIGSNSNLVAPLELDQHSFVAAGSTVTDDVPEHAMAIARARQVNKDNYWQKLPLAQDPDWL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should contain one or more MobA-like NTP transferase domains that are essential for its function.2. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.3. The protein requires Mg(2+) to activate a critical catalytic site.4. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc)5. The designed protein should have a binding site that is specific to the target UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA ligand.6. The protein design should take into account the physiological conditions of the UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway.7. The designed protein should be involved in lipid A biosynthetic process, UDP-N-acetylglucosamine biosynthetic process, cell morphogenesis by localizing to cytoplasm and exhibiting magnesium ion binding. The designed protein sequence is ",
    "response#1": "VVILAAGMGKRMQSALPKVLHPLAGKPLLAHVLDTARSLATNRVCVIYGHGGAAVLELLGKQPQPVATAQQEQQLGTGHAVMQALPELDDNAPTLILYGDVPLTTAASLQALIDAAGTDKLGILTVVQDPKGLGRIIRNDQGQVLAIVEEKDASIEQKKICEINTGILAVNSQLLHEWLPQLSNNNAQGEYYLTDIIAMAAMERGVTLLAPETVFFAHDTRVDADVTLEQNVVFGPGVSVESGSVIRAFSHLEGARVGAGSTVGPFARLRPGADLSDGAHIGNFVEIKNAKVGEGAKVNHLTYIGDATVGAGSNIGAGTITCNYDGFGKHHTDIGPGSFIGSNSTLVAPVTLGKGAYVAAGSVVTEPVEADALVIGRARQQTILARWHQPEK",
    "response#2": "GIVLAAGEGTRMKSARPKVLHEVAGLSLVGHALTAAHKAGVTDLAAVIGPDRADVAAEVGRLVPSAKVFEQRDRRGTAHAVLQAESVLAKGFDAVVILFGDTPLVRPETISALGEAVQGNNAIAVMGFTAKDPTGYGRLITSGASLEAIREADGDIARIVEEKDATEAQRAITEINSGILAFDVAALRGALGKLSNDNAQKEVYLTDVIGMARADGAAVHGHVAADASLVEGVNDRVQLAALGAELNRRIVRGWMLAGVTLVDPASTFVDAQVEIGRDTVIMPFTVISGSTSIGQSCNIGPYVQIRDSSVGDRCNIGPFAFLRPGTVLGDRVKVGDFVEVKNSRVGEGTKVPHLSYIGDAVLGSGVNVGAGTITCNYDGVAKHTTVIDNGAFVGANSNLVAPVRVGQNAYIATGSTVTTNVPPEALAIARARQRNIEGWVQRKRPGTAAAEAAQRAQAQPEDA",
    "response#3": "GIVLAAGEGTRMKSARPKVLHEVAGLSLVGHALTAAHKAGVTDLAAVIGPDRADVAAEVGRLVPSAKVFEQRDRRGTAHAVLQAESVLAKGFDAVVILFGDTPLVRPETISALGEAVQGNNAIAVMGFTAKDPTGYGRLITSGASLEAIREADGRLRAIREQKDASEAERAVTLCNSGLMAVRSEDLFGLLARVGNDNAAGEYYLPDVVALAVAEGRDAAVIETDAAEVAGVNSRAELAGVEAEWQRRRREALMEDAGTTVVDPPTTWIDADVTLEPDTTIAPHTFLHGRTHLARGATVGPECTLTDTIVGAEASVVRTTADRAEIGAGATVGPYSHLRPGTRLGREGKIGSFVETKSAEFGDHTKVPHLAYVGDAVVGERSNIGCTTVFVNYDGVAKHRTVIGSDVRIGSDTMLVAPVTVGDGAYTGAGSVIREDVPPGALAIREGRQVTKPGWAERFRAIMAAKKKARK"
  },
  {
    "reference": "MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDHQYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFAPFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNENILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFAGKATVLDAVEDSSIEAGLRRVLAETSQYITNEFDFTEAEQAGYVEKIIDRFNNSYLSDEVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKNNYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be designed to localize specifically to cytosol in order to perform its role in mannitol catabolic process more effectively.2. The NAD(+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MGEADRHTIDGIGIPSLVLMERAALQCLHVIAARQIDTGNVLIVCGSGNNGGDGFALARLFLEKGVHPTVTFVGKEASRSEECTTQMEIFKTLGGEIFSEIPEKDYTLVVDAIFGVGLSREISGRYATILETMNQLKGIKLAMDIPSGVSAENGQILGIGFRADYTVSMQCEKLGTVLFPGKEYAGEVFAVPIGII",
    "response#2": "VLLIAGSEGMSGAAFFSAAAAYGIGAGLVKIYTPESNRQVLQQLLPEAIITTYEKESVSSVTEWADVIGIGPGLSKGKKAAEILETVLTQAEVPCVIDADGLNLLAEQNRLELLKTRKQATVLTPHMKEMSRLLGGKPVAEIQTGRFEILKKFVEEYSVTCVLKDARTLVMKQDEHPYLNTTGNQAMAKGGSGDVLTGVITGLLAQRISAYDAAITGVFLHGMAGDQAKKEKGSYGVLARDLIHEL",
    "response#3": "MNSHKGTYGKVLLIAGSEGMSGAAFFSAAAAYGIGAGLVKIYTPESNRQVLQQLLPEAIITTYEKESVSSVTEWADVIGIGPGLSKGKKAAEILETVLTQAEVPCVIDADGLNLLAEQNRLELLKTRKQATVLTPHMKEMSRLLGGKPVAEIQTGRFEILKKFVEEYSVTCVLKDARTLVMKQDEHPYLNTTGNQAMAKGGSGDVLTGVITGLLAQRISAYDAAITGVFLHGMAGDQAKKEKGSYGVLARDLIHEVLLIAGSEGMSGAAFFSAAAAYGIGAGLVKIYTPESNRQVLQQLLPEAIITTYEKESVSSVTEWADVIGIGPGLSKGKKAAEILETVLTQAEVPCVIDADGLNLLAEQNRLELLKTRKQATVLTPHMKEMSRLLGGKPVAEIQTGRFEILKKFVEEYSVTCVLKDARTLVMKQDEHPYLNTTGNQAMAKGGSGDVLTGVITGLLAQRISAYDAAITGVFLHGMAGDQAKKEKGSYGVLARDLIHEL"
  },
  {
    "reference": "MQTPDLTTDNIETYLAEQSAKDMLRFITCGSVDDGKSTLIGRLLYESKLIFDDQLSALEADSKRVGTQGGNIDFALLVDGLAAEREQGITIDVAYRAFTTPNRKFIVADTPGHEQYTRNMATGASTADLAVLLVDATKGILTQTRRHAYIVTLLGIRQLVLAVNKMDLVENDEATYRAIEKDFLTFAKEIGAGDVTCIPMSALTGCNVTEKGPETSWYDGPCLLQHLETVDMLQHDHDQPARLPVQWVCRPDRTFRGFAGTLVSGTLKQGDKVKVLPSARESTIARIVTHDGDLDHAQSGQAIMLTLADEIDASRGNVICSADAPVEVADQFEAQILWMGDEPMLPGRPYLIKLGARTVPAKLAQPKYEVNVNTLEHTAAKTLELNQIGMCNISLDEELPFTSYTDNRDLGGFIIIDRASNTTVGLGLINFALRRASNIHWQALDIHKGTRAATKNQQSTVLWFTGLSGSGKSTVANIVEKRLTAMGHHTYLLDGDNVRHGLNRDLGFTDADRVENIRRVAEVSRLMADAGLIVLVSFISPFRAERRMARDMLEDGEFIEVHVDTPLEVCEARDVKGLYAKARAGEIKNFTGIDSDYEEPENAEITINTAEKSPEITAGEIVEHLENTGRLKPA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a high specificity for GTP binding, with minimal off-target effects.2. The Phosphoserine intermediate active site of the designed protein must be optimized for substrate binding and specificity.3. The presence of Tr-type G domains is critical for the interaction of the designed protein with its intended targets.4. The designed protein should be optimized for GTP binding, ATP binding activity in order to improve phosphorylation.5. A protein should be able to manipulate the activity of enzymes in the hydrogen sulfide biosynthesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "LLKFITCGSVDDGKSTLIGHMLYDAKLLFADQEKALELDSKVGSRGGEIDYSLLLDGLMAEREQGITIDVAYRYFTTDARSFIVADTPGHEQYTRNMAVGASFADLAVILVDATKGVITQTKRHARICYLMGIRHMVLAVNKMDLIKYDEETFRKINRDFDTFISSFADINTTVIPVSATEGDNIILNRKWY",
    "response#2": "RFITCGSVDDGKSTLIGRLLHDSQLIADDHLETLKTDSNNGTTGDNLDLSLITDGLKAEREQGITIDVAYRYFATRHRNFIIADTPGHEQYTRNMATGASTADLAVILVDARKGVLQQTRRHSYIAALLGVKHLIIAVNKMDLVGYSEERFSEIVAGYRDYSSNLGARSLEFIPISALAGDNVVEPSASMTWFEGPTLVDYLDSVEIQPALRFVLCGSVDDGKSTVIGRLLYDCRQIFSDELRRVEHDSARYGTQGKATDLALLVDGLRDEREQGITIDVAYRSFETPRRRFIVADAPGHEQYTRNMATGASTADLAVVLVDARKGIRPQTARHTRIAAMFGVRHAVLAVNKMDLVEWDREIFESIRDAYRELAAQIGLAAVEAIPVSALTGANLTRSGTSAPWYGGPTLLAHLETVDVEESPLRLPIQWVCRPEVSDQFKVTLVWMHEQAGLTGRNYELKLASQWVGASITAIKHRINVNTFAHESARDIGMNDIAVCTIATSKPVAFAPYEQSSTLGGFLLVDSFTKNTVAAGIVTDRFTSSLLWMDDEPLTEGKNFLVKAGTKTIPGTVTAIHHKTDINTGDQVDVSRGEKNELLTVEVVTAEKGVFDSFRNNETLGAFILIDRVTNSTAASGVLRRG",
    "response#3": "RFITCGSVDDGKSTLIGRLLHDSQLIADDHLETLKTDSNNGTTGDNLDLSLITDGLKAEREQGITIDVAYRYFATRHRNFIIADTPGHEQYTRNMATGASTADLAVILVDARKGVLQQTRRHSYIAALLGVKHLIIAVNKMDLVGYSEERFSEIVAGYRDYSSNLGARSLEFIPISALAGDNVVEPSASMTWFEGPTLVDYLDSVEIRELLRTVIVGHVDHGKSTLVGRLFHDTGSLPDGKMEAITEMCRKRGMPFEWAFLMDAMQAERDQGITIDTSQIWFHTEKRDYTIIDAPGHTEFLKNMVTGAAQADAALLVIDAGEGVQEQSRRHGYLLSLMGVRQVAVAVNKMDRVDYSQARFDEIEAEYRQYLNSVGVEATFVVPVSAREGDMIAMRTDGDEKANMDWYEGPTVVEAFDSFKMPEPFRMPVQWVNRPNLDFRGFAGMIAAGTVRPGDAIRVQPSGKTSRVRRIYTHDGDLEQAIAGQSVTVLLEDEIDISRGDVLEVADQLKVRIIWMDETPLFAGRHYDLYSAGGTARAEITRLRNRIEPESYRRLAAHTLEKNDIAEAEISLSRPIPFDPYGENRETGAFILVDRLTNATLCCGMEVADQLKVRIIWMDETPLFAGRHYDLYSAGGTARAEITRLRNRIEPESYRRLAAHTLEKNDIAEAEISLSRPIPFDPYGENRETGAFILVDRLTNATLCCGMINHSLRRADNIHWQALDVDKAARAAQKGQKPCVLWFTGLSGAGKSTIANLVEKKLHAMGVHTYIL"
  },
  {
    "reference": "MNYKTSFARTWTSIAVLCIATPGFAQVEESAIPLGEMNTEKEAASKDAPASKCPVMGSFTDASRRHTAAGVYQNGDWWPNQLNLQILHQNSSKSNPMGSDFDYAEAFQELDLDAVKKDIHELMTTSQDWWPADYGHYGPFFIRMAWHSAGTYRVADGRGGAGYGTQRFAPLNSWPDNGNLDKARRLLWPIKQKYGKNISWADLMVLTGNCALESMGFETFGFAGGREDVWEPQQDIYWGPETEWLGDERYSGDRELENPLAAVQMGLIYVNPEGPNGNPDPLAAAKDIRDTFGRMAMNDEETVALIAGGHTFGKAHGAADPSKYVGAAPEGASIEEQGLGWKNSYGSGNAGDTITSGLEGAWSATPAEWSHEYFENLFAYEWVLTKSPAGAHQWTPKDESAKGTVPDAHDPSKSHAPMMFTTDLALKVDPIYGPISKRFYENPDEFEQAFAKAWYKLTHRDMGPYSRLLGSEVPEPQLWQDPVPAVDHELIDDQDIAQLKTKILASDLSVSQLVETAWASASTFRGSDKRGGANGARIRLAPQKDWAVNQPAELANVLQQLEKIQKDFNGSQTGDKRVSMADVIVLAGVAAVEQAAKNGGHDVEVPFAPGRTDASQEQTDVEAFEVLEPTADGFRNYVGHGKLDRPAAELLVDRANLLTLTAPEMTVLIGGMRVLNANADNMKLGVFTEQTETLSNDFFVNLLDINTVWKKSEVCEHFYEGRDRETDEVKWRASSVDLVFGSNSQLRAIAEVYASDDSQSKFVNDFVA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must have at least one Plant heme peroxidase family profile domain.2. A protein with metal ion binding is needed to enhance hydrogen peroxide catabolic process in a specific context.3. The protein must exhibit the following characteristics: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity..4. The presence of heme b is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "FGNYGPLLIRMAWHSAGTYRVSDGRGGAGAGQQRFAPLNSWPDNASLDKARRVLWPVKKKYGRKISWADLMVLSGNVALETMGLKTFGYAGGREDAWEAEEDVFWGPETAWLDDERYSGERDLEKPLAAVQMGLIYVNPEGPNGNPDPIAAARDIRETFARMAMNDEETVALIAGGHTFGKTHGAAPDGLVGPEPESAPLEDQGLGWKGVHGTGKGRDSHTSGLEVTWTPTPTTWDNSFWDVLFGYEWELTKSPAGANQWRPKNGGGANTVPDPEDGTLNRQPEMLTTDLSLRFDPIYGPISQRFRS",
    "response#2": "LDKARRLLWPIKQKYGQKISWADLMILTGNVALESMGFKTFGFAGGRADVWEPDEAVYWGSETTWLGGDERYSDGSEGVPKDHGVVSSDDDADGHVHSRNLENPLAAVQMGLIYVNPEGPDGNPDPIAAAKDIRDTFSRMAMDDEETVALIAGGHTFGKTHGAASSDHVGKEPEASGLELQGFGWQNSFGSGKGADAITSGLEVTWTKTPTQWSNNFFENLFGFEWELSKSPAGAHQWVAKNAEAIIPDAFDSSKKHLPTMLTTDLSLRLDPAYEKISRRFLENPDEFADAFARAWFKLTHRDMGPRERYLS",
    "response#3": "AFARAWFKLTHRDMGPRARYLGADVPAEELLWQDPIPTLDHALVDAQDIAALKQTLLGSGLSIAQLVSTAWAAASTFRGSDKRGGANGARIRLAPQKDWEVNQPEQLVQVLATLERIQAQFNGAQRGGKKISLADLIVLGGAAAVEQAAKNAGQTLTVPFVPGRMDATQAQTDVASFAVLEPIADGFRNYSKRRYAVPAEALLIDKAQLLTLTAPEMTVLVGGLRVLGANVGQSAHGVFTKRSDTLSNDFFANLLDMGIEWKATSDTREVYAGRDRASGEQKWTGTRADLVFGSNAILRALAEVYARL"
  },
  {
    "reference": "MASLGFLFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRAKAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKAGTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSSNPLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYRLRVASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYWISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMGSPKPPPQNHRRIILLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYGINAFDTKPPPDNFPKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFWVLGYGEGKFSEKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHLHMGMGVIFAEGVHLVKKIPKEALACGLTGKMLMSNKHN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should incorporate a binding pocket that specifically recognizes Cu cation to enhance enzymatic efficiency.2. The designed protein should have a binding site that is specific to the target Cu cation ligand.3. The designed protein must be optimized for copper ion binding, L-ascorbate oxidase activity.4. The protein should be designed to have high stability and activity towards 4 L-ascorbate + O2 = 2 H2O + 4 monodehydro-L-ascorbate   radical.5. For general function, the protein need meet that May be involved in a redox system involving ascorbic acid. The designed protein sequence is ",
    "response#1": "DMQYGRHIDNPFMSSGRADLSFTISLTDKNTYDGGELIIEEMNSEKEFKLNAGEIIIYPSTYLHSVKEIKNGERLVCVGWR",
    "response#2": "MNFLTNQLLIQEEIEALIKNLNKENTLWEDGKKTAGSHASMVKNNLQLNRESEISKKLSHLIKKKILSSPLIKSFALPKIIHGIMFTKSLKDMQYGRHIDNPFMSSGRADLSFTISLTDKNTYDGGELIIEEMNSEKEFKLNAGEIIIYPSTYLHSVKEIKNGERLVCVGWIR",
    "response#3": "EINLLLSDWWHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYL"
  },
  {
    "reference": "MRTAHVVETVRTAEHALMARLPDGTLMHRAAFGLASVCAQILPRVSGARVVLLVGSGDNGGDALFAGAELARRGAAVTAVAAGRTPHPGGLAALRAAGGRTAAVAEAADLVAAADLVVDGLVGIGVRGALREPHASLAALTETTDAAVVAVDLPSGVDADTGAVPGASVRADVTVTFGTHKPGLLVDPGAQRAGVVEFVDIGLAPDLPAPDVVAPQAADVAALLPRPTAESDKYRRGVLGLAAGSDRYRGAAVLAAGGALRTGVGMLRYAGQPEVAREVVARWPEAVVSVLDPMDPVASLPRRVAAWVIGPGRGLHPTAAAELEAVLATEEPVLVDADAITLLARDPDLVRKRDAPTLLTPHAGELSRLLPGADRADIEANRLEHARRAAAEYGCTVLLKGSTTLVAEPGRPVAANPTGTPLLATAGSGDVLAGMIGALLAAGLPSWEAAVCGAYLHGLAARLAHDGAPISASDLFDAVPRAIAAVTRPGE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be optimized for metal ion binding activity in order to improve nicotinamide nucleotide metabolic process.2. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration3. The designed protein should be able to compete with endogenous proteins or inhibitors for K(+) binding. The designed protein sequence is ",
    "response#1": "LSAETAAGCIPKRQEDSNKGDYGKLLNIGGCVHMTGAVILSTLSAMRCGVGLIKVATPQPVISAVASHILECIYSPLPVSESGSISIDGAEQLRTEMGWATAGLIGCGLSVCDDTRLLVEEVISSFGRPLVIDADGLNCVAENPAMLRKALAPVIITPHIKEMSRLTGLSVEIIKNRRFDVAAQFAEKYGVTVVLKDSNTVIATPKRELFINGNGNSGLAKGGSGDVLAGVIASFLAQGASPLAAAVAGVFLHAEAGDMAAQTFTPYAMLPSDVIESIPFVLD",
    "response#2": "VEEHDLRCLPPRRPSGNKGTFGKVLVVAGSEGMCGAAYLSAAAALKAGAGMVQIQTVEANRIPLQILLPEAMVSCTFTEEENSRILSWCDVVVIGPGLGNYGLSYERELWFLKKAGELHKPVILDADGLNLLALRPKWKEYLGEHVVVTPHIGEMSRLTGKTIGEIQNCMAETALSYAEETKTTCVLKDACTVTAGREGSLYLNLSGNEGMATAGSGDVLTGILAAVFCMYLDGEASVSSEIKAALGVYLHGRCGDIAAEKYGSRSLTATNLIEALPEAFCRR",
    "response#3": "MYEADRLAMQSGIPGLRLMEAAGLAIERVIRGAWSPRPVVVLAGPGNNGGDGFVVARLLERRGWPVTVALLGDRARLKGDAAANAARWKGPCIPLSPEVLNRGALVVDALFGAGLARPLEGGTAATIEELNRRELPCVAVDVPSGIHGDSGSVLGCAPRCLATVTFFRPKPGHLLEPGRQYRGALTVADIGIPGSVLPGHKYDHGHALVLTGGAGRTGAARLSARAALRIGAGLVTLGVPGAAQLEVAMHETAVMLRRVDDAEALDTALEDPRLNSLCLGPGLGSERAHALVPAALASGRATVLDADALTAFADAPDRLFSKLHARCVLTPHMGEFARLFPDIAEDLKRTPESGPAASRIDAARAASVRCGAVVLLKGADTVIAAPDGRVLVNAAVYERSVPWLATAGAGDTLAGLIAGLLARGIAPLEAAGTGAWLHVECARRFGPGLIAEDLCGMLPGL"
  },
  {
    "reference": "MAAGRLLLYAGLSLALCALGMLAVAICSDHWYETDARKHRERCKSITTKRNDPGFIYNSNNNLPLRASRSRLDRWEGKLLLARNRRQIFAMSAVDECNRQYNSTNMGLWRKCHRQGFDQELEELIAKGAIERCSYIKYHYSSATIPKNLTYNITKTIRQDEWHALHLRRMTAGFMGMAVAIILFGWIIGVLGCCWDRGLMQYVAGLLFLMGVITCYEELHAKKPRFLSLLHYQIHEGAAFLTKYPLRRYFLQRFHHAFGNQLIPATVPLKKATVFLNPAACKGKAGNLFEKNAAPILHLSGLDVTVVKTDYEGQAKKLLELMENTDLIIIAGGDGTVQEVITGLLRRADEAAFSKIPIGFIPLGKTCTLSHTLYPESTNQVQHITNATLAILKGETVPLDVLQIKGEKEQPVFAVSGLRWGSYRDAGVKVSKYWYLGPLKTKAAHFFSTFKEWPQKHQAALMYLGPTERPPEEPDEKPSRPPLYVRLYRRLRSYWSSPKEFPQEVAPEDWEELKLSTIELSIATRNRQLDLTRTEDFMDICIEAESISKGEFVHRGSQKMRDPHMCPEGSQCIQASRCILQLPEGAEGSFGIDNEEYEAMPVEVKLLPRKLRFFCDPRMREQMLQAAVQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein can be expressed in mitochondrial intermembrane space and perform kinase activity without triggering undesirable cellular responses.2. We require a protein that can specifically bind to the metabolites produced in the glycerolipid metabolism pathway. The designed protein sequence is ",
    "response#1": "MTGLRWGSYRDAAVKVSNYWYLGPLKTKAAHFFSTFKEWPQRHQAALMYLGPAERPSEEPEEKRSRPPLHVRLYRRLALYWSPPPKEIPQEVTQDNWEELKISTIELSIATQNRQLDLTRTEDFMNICIEADTVSKGQFVNRGSQKMSDPQMCPEGSQCIQASKCILQLPEGTEGSFGIDNEEYEAMPVEVKLLPRKLRFFCDPRRREQMLRA",
    "response#2": "MTGLRWGSYRDAAVKVSNYWYLGPLKTKAAHFFSTFKEWPQRHQAALMYLGPAERPSEEPEEKRSRPPLHVRLYRRLALYWSPPPKEIPQEVTQDNWEELKISTIELSIATQNRQLDLTRTEDFMNICIEADTVSKGQFVNRGSQKMSDPQMCPEGSQCIQASKCILQLPEGTEGSFGIDNEEYEAMPVEVKLLPRKLRFFCDPRRREQMLRV",
    "response#3": "MTGLRWGSYRDAAVKVSNYWYLGPLKTKAAHFFSTFKEWPQRHQAALMYLGPAERPSEEPEEKRSRPPLHVRLYRRLALYWSPPPKEIPQEVTQDNWEELKISTIELSIATQNRQLDLTRTEDFMNICIEADTVSKGQFVNRGSQKMSDPQMCPEGSQCIQASKCILQLPEGTEGSFGIDNEEYEAMPVEVKLLPRKLRFFCDPRRREQMLRN"
  },
  {
    "reference": "MKRPEKEAVVAELTEQFRNASAVYLTEYRGLTVPQISDLREKLGRDTSYTVAKNTLFRIAAKEAGIEGLDEMLKGPSAVVFVDGDFIEAAKVLRDFAKTNKALIVKGGFADGTVYDAEGAKQLADLKSRPELLAELAGALKGTMSKAAYLFNALPTKVVRTIDALRDKQEKAA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors..2. The designed protein must be able to regulate translation. The designed protein sequence is ",
    "response#1": "IPSAAALIMKEAGIQKGSGASGTEWAGDVTMDAVVKVANTKLENSYASSLKSVAKTIVGTCLALGVKL",
    "response#2": "IPSAAALIMKEAGIQKGSGASGTEWAGDVTMDAVVKVANTKLENSYASSLKSVAKTIVGTCLALGVKN",
    "response#3": "IPSAAALIMKEAGIQKGSGASGTEWAGDVTMDAVVKVANTKLENSYASSLKSVAKTIVGTCLALGVKS"
  },
  {
    "reference": "MVYLAVGIAGMIGALVRYGLGLVVPAAAVGGFPLGTLFINWTGSFLLSWFTVMFTRRPAWPPWLKTAVTTGFVGSYTTFSTLSVECVELMEQGRFGMAAVYIAASLFGGLLASWAGYAAAQPERKEGIG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Important for reducing fluoride concentration in the cell, thus reducing its toxicity.2. The designed protein should have a specific function within the plasma membrane. The designed protein sequence is ",
    "response#1": "MPFAP",
    "response#2": "MFRL",
    "response#3": "MF"
  },
  {
    "reference": "MLGFLRQPNLPDFDGKCVSHTVLFADGTRESLGVIFPSTLTFSTGAPERMEILAGHCRVRLAGAAEWTHYVAGQAFDAPGDSSFDIQTLERVDYVCHFG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates. The designed protein sequence is ",
    "response#1": "GAPGCR",
    "response#2": "MN",
    "response#3": "GNWAR"
  },
  {
    "reference": "MLPLTMTVLILLLLPTGQAAPKDGVTRPDSEVQHQLLPNPFQPGQEQLGLLQSYLKGLGRTEVQLEHLSREQVLLYLFALHDYDQSGQLDGLELLSMLTAALAPGAANSPTTNPVILIVDKVLETQDLNGDGLMTPAELINFPGVALRHVEPGEPLAPSPQEPQAVGRQSLLAKSPLRQETQEAPGPREEAKGQVEARRESLDPVQEPGGQAEADGDVPGPRGEAEGQAEAKGDAPGPRGEAGGQAEAEGDAPGPRGEAGGQAEAEGDAPGPRGEAGGQAEARENGEEAKELPGETLESKNTQNDFEVHIVQVENDEI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The presence of EF-hand domains is critical for the interaction of the designed protein with its intended targets.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in extracellular region that is critical for regulation of cell cycle, cell adhesion.3. The protein must contain a signal peptide for proper functionality. The designed protein sequence is ",
    "response#1": "RYGTISWAPTTKYNNIKITVNLAFRTGYWGSVAIGQQISHGNIDFGDNTKTGLTMTVTSKDTDNEWFTATWSGIHEYSPQPTAQTKNYIVVYKDCCRISTLLNNGDQYWHISAGIQIQ",
    "response#2": "VTNMMPIVRVIANKNNNFRIIANDQNKQTALSYKFTDTWTMDQPPGMTVDSINGNCWFLPTKEGLYSTQIKVTDALGAYVVVDFLLQTYTEVGKCDPTCSNADASCTSNSQCTKCSC",
    "response#3": "NFKTLSTMEDFWKFTEGSLLDGLYWEMWYSNRTGAENRSFIYYENLLLGVPRIRQLKVRNGSCSIPPDLREEIKECYDVYSISNEERAPFGLRHGTAWTYTNEKDLNGSSHWGLIATYSGAGYYLDLSRTREETAVQIANLKKNIWLDRGTRATFIDFSVYNANINLFCVVRLLVEFPATGGVITSWQFQPVKLF"
  },
  {
    "reference": "MSQFENVTAVKRANVYFEGKVTSRTVLLPDGSKVTLGIMLPGEYEFGTDSKEIMEILAGNLKVQLPGSDEWIHIQGEGEFTVPAHSRFKLEVLGVTDYCCSYISE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates.2. The designed protein must be able to perform uridine phosphorylase activity under specific conditions.3. The protein should have a low Km value for phosphate + xanthosine = alpha-D-ribose 1-phosphate +   xanthine. The designed protein sequence is ",
    "response#1": "SCGRF",
    "response#2": "EAPESQ",
    "response#3": "MSNRFSL"
  },
  {
    "reference": "MEWEIWESYRAAKEAAVRRKYDQSALFLRTALSLVDCRLNGLSTDDPNRDQLVKVKSVLKLNVERMNGIADVVSQMRQMVGVATLSSPIDAPPSDPDVWPLPPLTKKTSTILSPKSTNKKQTSSRVNNKAKKGSVGKSISAGGRIITKRDNSLGRASMNELSEVTSKEEGDNNSNEKGENEKVFDGRGFDKELVEIIERDIMQKRPDVHWDDIAGLDEAKKLLKEAVILPSVMPNFFKGIRRPWRGVCMVGPPGTGKTMLAKAVATESQTTFFCVSSATLTSKYRGDSEKLVQLLFKMARFYAPSTIFIDEIDSLCSRRGADSEHEASRRVKSELLTQMDGCSPDVSRVLVLAATNFPWDLDEALRRRLEKRIYIPLPDKTNRFQLLKLALAEVSIDDEVNLETVADSLNGYSGADITNVCREAAMMSMRVRIANLTAEEIKALTQEEVDLPITSSDFSQAIQNTSPSVSYFDVQKYEKWIHDYGAA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should contain one or more AAA+ ATPase domains that are essential for its function.2. The protein must have high specificity towards isomerase activity.3. The protein should be designed to have high stability and activity towards n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1)   alpha/beta tubulin heterodimers.. The designed protein sequence is ",
    "response#1": "PWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPTTIFIDEIDSICGRRGTSDEHEASRRVKSELLIQMDGVGGALEGDDPSKLVMVLAATNFPWDIDEALRRRLEKRIYIPLPTAEERAAIIRAA",
    "response#2": "VLMVGPPGTGKTLLAKAVATECRTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPTTIFIDEIDSICSRRGNSEEHEASRRVKAELLVQMDGVGGTSENDPSKMVMVLAATNFPWDIDEALRRRLEKRIYIPFDLDLAEIAEKLEGYSGADLTNVCRDAAMMSMRQRIAGLEMDEDLDFDEISSLLHGYSGADVANVCRDASMMSMRRRIAEIGTDEIEKAKNIAKEELDLPVTQADFLEALQKCSKSVSEADIKKYVDWMDEFG",
    "response#3": "PYRGVLMVGPPGTGKTMLAKAVATECKTTFFNVCSSSLTSKYHGESEKLVRLLFEMAHFYAPSTVFIDEIDSVCSKRGTSNEHEASRRVKSEILVQMEGVSTTQNADSDPSKMVIVLGATNFPWDIDEALRRRLEKRIYIPLPTLEVEQVKRLDVSAVRSITEQDFYNSLKRIRRSVAPQSLISYEKWSQDYC"
  },
  {
    "reference": "MTTLYSDWATESFEYDESSEACFIGDIVAFGTIFLSIFYSLVFAFGLVGNLLVVCALTSSRKPKSITDIYLLNLALSDLLFVATLPFWTHYVISEQGFHNAVCKLTTALFFIGFFGGIFFITVISIDRYMAIVLAANSINNRTVQHGVTTSLGVWAAAILVAAPQFMFTKQKGNECLGDYPEVLQDIWPVLRNTEANFLGFLLPVLIMSYCYFRIIQTLFSCKNHKKAKAIKLILLVVIVFFLFWTPYNVMIFLETLKLYGFFPNCDMKRDLRLALSVTETVAFSHCCLNPLIYAFAGQKFRRYLRHLSRKCQAVLCGRPVHVSFSPSESQRSRQESIVSSNFTHYTSDGDASLLL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should contain Cytoplasmic, Extracellular topological domains.2. The protein must exhibit the following characteristics: Acts as an important regulator of the gut microbiota by controlling immunity to intestinal bacteria and fungi; Involved in airway inflammation by promoting interleukin 2-producing T helper (Th2) cell survival in inflamed lung; Involved in the migration of circulating monocytes to non-inflamed tissues, where they differentiate into macrophages and dendritic cells; Synaptic pruning by microglia is required to promote the maturation of circuit connectivity during brain development; Acts as a regulator of inflammation process leading to atherogenesis by mediating macrophage and monocyte recruitment to inflamed atherosclerotic plaques, promoting cell survival; CX3CR1-CX3CL1 signaling mediates cell migratory functions; Responsible for the recruitment of natural killer (NK) cells to inflamed tissues; Required to restrain the microglial inflammatory response in the CNS and the resulting parenchymal damage in response to pathological stimuli.3. The protein should have a Helical; Name=3, Helical; Name=6, Helical; Name=4, Helical; Name=7, Helical; Name=2, Helical; Name=1 domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "GNSLVMLVILYRRRTRSVMDVYVLNLAIADLLFSLTLPFLAVSKLKGWIFGTPLCKMVSLLKEFNFFSGILLLACISVDRYLAIVHATRTLARKRYLVKFVCVGIWGLSLILSLPFAIFRQAYKPFRSGTVCYEVLGEATTDFRMTLRGLSHIFGFLLPLLTMLVCYGLTLRMLFKTHMRQKHRAMGVIFAVVLVFLLCCLPYNLVLLSDTLLGAHLIEDTCERRNDIDQALYITEILGFSHSCLNPIIYSLSHPYFKDTLKGLLGCCGEDRGNHVH",
    "response#2": "ANLLALRAFVSRVRQPQPAPVHILLLNLTLADLLLLLLLPFRIVEAASNFRWYLPKIVCALTGFGFYSSIYCSTWLLAGISIERYLGVAFPVQYKLSRRPLYGVIAALVAWIMSFGHCTIVIIVQYLNSTEQVGTENQITCYENFTQAQLDVVLPVRLELCLVLFFVPMTVTIFCYWRFVWIMLTQPHVGAQRRRRAVGLAVVTLLNFLVCFGPYNMSHLVGFHLRQSPSWRVEAVVFSSLNASLDPLLYTLCNRAFRADFLNLLRCPSFRRRSRSSTSSSGSTPTIHTYVH",
    "response#3": "GNAMVAWVAGKVARRRVGATWLLHLAVADLLCCLSLPILAVPIARGGHWPYGAVGCRALPSIILLTMYASVLLLAALSADLCFLALGPAWWSTVQRACGVQVACGAAWTLALLLTVPSAIYRRLHQEHFPARLQCVVDYGGSSSTENAVTAIRFLFGFLGPLVAVASCHSALLCWAARRCRPLGTAIVVGFFVCWAPYHLLGLVLTVAAPNSALLARALRAEPLIVGLALAHSCLNPMLYALCNATFKKTFKDLLLCQW"
  },
  {
    "reference": "MLSIDPLRKPPDIDIKLGGSKSITNRSLIVAALAKGTSTLHGALFAEDTYAMIDCLRRLGIQIECDEVAKSIVVVGSSGHLRSTGETLWVRQSGTTARFIMPLAALSGGEIRIDGDPQILNRPHQELFAALQELGVLITFDEKENHLPATINGSDLVAKEVFLNAEISSQYVSALLLAAPCMPEDLIVSVEGKLVSESYLAMTLSVMRSFGAVIEEVDKNKFLVRSTGYISHDYQIEADASTSSYFFAVAAMTKGRVKVEGLGRSSIQGDVQFVDILEEMGAEVSRSEEAIEVRGTSSLKGLSVSMESISDTAPTLAVIAPSAETTTSVTDIGFIAYKESNRIEAVVAELQKLGIEASENSTGFTISPGEVNSGVVHTYDDHRIAMAFTVLGLISPGISLDNPECVSKTAPDFFDHVDLLRLEGDRELAILAIDGPAGSGKTSLAKRLASELDFEYLDTGAMYRSVAARVLEEGINPEDQHVVTKIANQTSIVFKGEEVLVDGQNFTDVIRSPEVNSVVSYVAANPGVRAVLRRAQRSWARKRGGGVLEGRDIGTVVFPKAKLKVYVTASPEERAKRRSLQSGRPVKEIMDEITGRDKIDSSRVDSPLSVSSGALIVDTTGKTIEEVVEEILERFHV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must have at least one Enolpyruvate transferase domain.2. The designed protein should have cytidylate kinase activity to facilitate aromatic amino acid family biosynthetic process, phosphorylation.3. The protein must exhibit the following characteristics: Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate..4. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in ATP + dCMP = ADP + dCDP are properly positioned for optimal catalysis.5. The designed protein should have a high specificity for 3-phosphoshikimate, ATP binding, with minimal off-target effects.6. Ideal protein should have a binding affinity for key enzymes in the Metabolic intermediate biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "IDGPTASGKGTLAARVAQQLGYHLLDSGALYRITALAAARAQLPLDTSGAAALARLAQELPIAFDGDRVLLGGEDITEAIRSEDVGMNASRISALPAVRAALVDLQHSFRQLPGLVADGRDMGTVIFPDAPLKVFLTASAEKRAQRRHKQLISKGISTTLAALCADLQARDARDTSRSASPLQPAPDALLLDNTGLDVAHSVRGSVRLPGSKSISNRVLLLAALCEGRTVVHDLLDSDDTRVMLDALRALGCGVQVDGSRATIDGLGNRPRQPQAELFLGNAGTAMRPLTAALAVLGGDYQLRGIPRMHERPIGDLVDALRALGCTIDYLGTPGYPPLRIGQPAVRLDQPIGVRGDVSSQFLTALLLALPLAASQPGRRDVVIEVVGELISRPYVEITLQLLARFGVQVQREGWSRFTIPQGSRYRSPGDIHVEADASSASYFIAAGAISEPGRGPNGLKIEGVGEQSIQGDIRFVDAARAMGAEIDSGPNWLQVRRGAWPLKAIDLDCNHIPDAAMTLAAMALYADGPSTLRNIASWRVKETDRIAAMATELRKLGAQVEEGTDFIRVTPPDSTTGWRAASLHTYDDHRMAMCGSLAAFNPARLPVRIQDPRCVGKTFPDYFETLFARHD",
    "response#2": "VCIDGPTASGKGTLAAEVARRLGYHYLDSGALYRVTGLAVRRMGLEPEPRHEAAIAQAAATLPLSFTAGQVWLAGEDVTEAIRSEAAGMDASRVSALASVRAALRTLQEGFRKLPGLVADGRDMGTVIFPAAALKVYLTASAAQRAERRHKQLISKGISTTLDSLRADLEARDARDSSRSVAPLKPAQDARLLDNSTLSIEQSVEQVLDTGAGLSPQAVMEFLLARVGAQ",
    "response#3": "VDGPTASGKGTLAAAVAHRLGYHYLDSGALYRLTGLAARQAQLEPEPVNEAEIAALARQLPVQFRADRVWLAGEEVTEQIRSEAAGMDASRVSALPSVREALLNLQHSFARLPGLVADGRDMGTQIFPFAPLKVFLTADAAQRADRRHKQLISKGISANIDSLRADLAARDARDSSRSVAPLQPAQDALLLDNTSADIDATVAAIEAALRT"
  },
  {
    "reference": "MENRWQVMIVWQVDRMRINTWKSLVKYHMHVSKKAKKWFYRHHYESHHPRISSEVHIPLGDAELVVTTYWGLHTGEREWHLGQGVSIEWRLKRYRTQVDPGLADRLIHMHYFDCFSESAIRKAILGHIVSPKCDYQAGHKKVGSLQYLALRALVAPKKIKPPLPSVRKLTEDRWNEPQKTKGHRENQTMNGH",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The ideal protein would be one that plays a critical role in viral life cycle through RNA binding within plasma membrane, host cell cytoplasm, cytoplasm, virion component, host cell plasma membrane.2. Incorporating a BC-box-like motif motif is critical for the successful design of the protein.3. For general function, the protein need meet that Finally, Vif also contributes to the G2 cell cycle arrest observed in HIV infected cells.; Recruits an active E3 ubiquitin ligase complex composed of elongin BC, CUL5, and RBX2 to induce polyubiquitination of APOBEC3G and APOBEC3F; Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G; Seems to play a role in viral morphology by affecting the stability of the viral nucleoprotein core; May interact directly with APOBEC3G mRNA in order to inhibit its translation; Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions; Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism; In turn, they are directed to the 26S proteasome for degradation4. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "MENRWQVLIVWQVDRMRIRTWNSLVKHHKYRSGKSKKWCYRHHYESSNPRVSSEVHIPLGEAKLVVTTYWGLHTGERDWHLGQGVSIEWRRKRYSTQVDPDLADQLIHLYYFDCFSESAIRKAILGQVVRPRCEYPTGHNKVGSLQYLALTALVTPKRRKPPLPSVKKLTEDRWNKPRKTKGHRGSHTMNGH",
    "response#2": "MENRWQVMIVWQVDRMRIRTWNSLVKHHMYISKKAKGWFYRHHYESRHPKISSEVHIPLGDAKLVVITYWGLHTGERDWHLGQGVSIEWRERRYSTQVDPDLADQLIHLHYFNCFSDSAIRKALLGHRVSPRCEYQAGHNKVGSLQYLALTALIKPKKIKPPLPSVKKLTEDRWNKPQKTKGHRGSHTINGH",
    "response#3": "MENRWQVMIVWQVDRMRIRTWNSLVKHHMYVSKKAKGWFYRHHYESRHPKVSSEVHIPLGDARLVITTYWGLHTGEREWHLGQGVSIEWRQRRYSTQVDPDLADQLIHLYYFDCFSESAIRNAILGHIVSPRCEYQAGHNKVGSLQYLALTALIKPKKIKPPLPSVRKLTEDRWNKPQKTKGHQGSHTINGH"
  },
  {
    "reference": "MLTFHRIIRKGWMFLLAFLLTALLFCPTGQPAKAAAPFNGTMMQYFEWYLPDDGTLWTKVANEANNLSSLGITALWLPPAYKGTSRSDVGYGVYDLYDLGEFNQKGAVRTKYGTKAQYLQAIQAAHAAGMQVYADVVFDHKGGADGTEWVDAVEVNPSDRNQEISGTYQIQAWTKFDFPGRGNTYSSFKWRWYHFDGVDWDESRKLSRIYKFRGIGKAWDWEVDTENGNYDYLMYADLDMDHPEVVTELKSWGKWYVNTTNIDGFRLDAVKHIKFSFFPDWLSDVRSQTGKPLFTVGEYWSYDINKLHNYIMKTNGTMSLFDAPLHNKFYTASKSGGTFDMRTLMTNTLMKDQPTLAVTFVDNHDTEPGQALQSWVDPWFKPLAYAFILTRQEGYPCVFYGDYYGIPQYNIPSLKSKIDPLLIARRDYAYGTQHDYLDHSDIIGWTREGVTEKPGSGLAALITDGPGGSKWMYVGKQHAGKVFYDLTGNRSDTVTINSDGWGEFKVNGGSVSVWVPRKTTVSTIAWSITTRPWTDEFVRWTEPRLVAWP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The ideal protein would be one that plays a critical role in carbohydrate metabolic process through calcium ion binding, alpha-amylase activity within extracellular region.2. The presence of Na(+) is necessary for the protein to function in the desired environment.3. The Proton donor, Nucleophile of the protein must be engineered to enable effective catalysis of Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in   polysaccharides containing three or more (1->4)-alpha-linked D-   glucose units..4. The protein design should prioritize the formation of strand-strand-helix-strand-strand-strand-strand-helix-strand-strand-strand-strand-strand-turn-helix elements.5. The protein should be able to bind Na(+), Ca(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MRKEAIYHRPADNFAYAYDSETLHLRLRTKKDDIDRVELLHGDPYDWQNGAWQFQMMPMRKTGSDELFDYWFAEVKPPYRRLRYGFVLYSGEEKLVYTEKGFYFEVPTDDTAYYFCFPFLLDGEWGFSYFDRPQAVPDAWINGDLPQVGRLSVPSNWQLSGYDAPIYTNVQYPIPTDPPRVPDDNPTGCYSLTFHCQPAWLQSGQTRIIFDGVNSAFYLWCNGEFIGYSQDSRLPAEFDLSQRLLEGENRIAVMVLRWCDGSYLEDQDMWRMSGIFRSVSLLHKQAPSSVLPKTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWREAHVRGLTRRHPGIPAAIRGTYAALAHPVMLDYLTQLGVTALELMPVQQHADEPRLQSMGLRNYWGYNTLLPFAVDNSLAASDDPLNEFRDTVRALHQAGIEVILDVVFNHSAELDVDGPTLTLRGIDNASYYWLTENGDYHNWAGCGNVLRLEHPAVLHWVIECLTFWHEVCHVDGFRFDLATILGRLPDFSSSAPFFTALRNHRSLRDCKLIAEPWDISPGGYQLGQFPAPFAEWNDRFRDDMRRFWLHGDLPIGVLARRFAASSEVFERGSRQPWASVNMLTSHDGFTLRDLVCFNHKHNDANGEQNRDGTNSNFSFNHGTEGLEADETTQARRRVSQQALLTTLLLSQGTPMLLAGDEFGNSQQGNNNAYCQDNALAWLHWDQADDALLAFTSGLIRL",
    "response#2": "MLLEAIFHEAKGSYAYPISETQLRVRLRAKKGDVVRCEVLYADRYASPEEELAHALAGKAGSDERFDYFEALLECSTKRVKYVFLLTGPQGEAVYFGETGFSAERSKAGVFQYAYIGRSTRYWDCCKPSCGWAKKAPVNQPVFSCNANFQRITDFDAKSGCEPGGVAYSCADQTPWAVNDDFALGFAATSIAGSNEAGWCCACYELTFTSGPVAGKKMVVQSTSTGGDLGSNHFDLNIPGGGVGIFDGCTPQFGGLPGQRYGGISSRNECDRFPDALKPGCYWRFDWFKNADNPSFSFRQVQCPAELVARTGCRGGTRGRSTRYWDCCKPSCGWAKKAPVNQPVFSCNANFQRITDFDAKSGCEPGGVAYSCADQTPWAVNDDFALGFAATSIAGSNEAGWCCACYELTFTSGPVAGKKMVVQSTSTGGDLGSNHFDLNIPGGGVGIFDGCTPQFGGLPGQRYGGISSRNECDRFPDALKPGCYWRFDWFKNADNPSFSFRQVQCPAELVARTGCRGGTR",
    "response#3": "MLLEAIFHEAKGSYAYPISETQLRVRLRAKKGDVVRCEVLYADRYASPEEELAHALAGKAGSDERFDYFEALLECSTKRVKYVFLLTGPQGEAVYFGETGFSAERSKAGVFQYAYIYYGLNHLSFIEKVFVKGEDVTEKVFENLKLKLSNIPDEDFPTWFYDSVRLIVNPYLRYYLMEKKMFKKISTHELRAREVMKIEKELFEKYRTAVEIPEELTKRGGSMYSTAAAHLIRDLETDEGKIHIVNTRNNGSIENLPDDYVLEIPCYVRSGRVHTLSQGKGDHFALSFIHAVKMYERLTIEAYLKRSKKLALKALLSHPGIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRMLDDEDDVRWLNAQGEALTPYEWEQGTHQLQIQLSERWLLLVNATPQVSDFSLPEGEWRVAPPFSATDHLLDGQTWRGQANAVCVDWDETNYLEAEPGEYITIARKAKDTDDWYVGCTAGENGHTSKLVFDFLTPGKQYIATVYADAKDADWKENPQAYTIKKGILTNKSKLNLHAANGGGYAISILL"
  },
  {
    "reference": "MRAKGTQMNWPNLWRWGTLMLGLVIICSASENLWVTVYYGVPVWRDAKTTLFCASDAKAHVTEVHNVWATHACVPTDPNPQELHLENVTENFNMWKNDMAEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTNADLTNATRRSSTTTTATTTTHSVNTTATTPNTTTVSTTTTTDGPNLGNISDEVRACSFNMTTEIRDRQKKVHALFYRLDIVPIEGKENSSITYQQYRLINCNTSAIKQACPKVSFDPIPIHYCTPAGYAILKCNDKKFNGTGPCNNVSSVQCTHGIKPVVSTQLLLNGSLAEDEIVIRSENLTDNAKTIIVHLNESVEINCTRPSNNTRKSVNIGPGQALFYQSGDIVGDIRKAYCQINRTRWNNVLKQVAEKLKKHFKNKTIIFQPPSGGDLEITMHHFNCRGEFFYCNTTELFNKTYNGTLNDDNNSTNITLPCRIKQIINMWQGVGQAMYAPPISGRISCVSNITGILLTRDGGLNNSADNETFRPGGGNIKDNWRNELYKYKVVQIEPLGIAPTRAKRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKTICTTAVPWNSSWSNKSYEEIWNNMTWIEWEREISSHTNQIYQILTESQNQQDRNEKDLLELDKWASLWNWFDITRWLWYIKIFIMIVGGLIGLRIVFAVLSIVNRVRQGYSPLSFQTPNLQQREPDRPERIEEEG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein's functional design necessitates the inclusion of a YXXL motif; contains endocytosis signal motif.2. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.3. The protein must exhibit the following characteristics: Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.4. The designed protein should have at least Helical transmembrane domains to ensure proper localization.5. A protein that contributes to positive regulation of plasma membrane raft polarization, clathrin-dependent endocytosis of virus by host cell by functioning in plasma membrane, host cell endosome membrane, viral envelope with structural molecule activity.6. The designed protein must have at least one Human immunodeficiency virus 1 envelope glycoprotein Gp120 domain. The designed protein sequence is ",
    "response#1": "MRVTGIRKNYQHLWRWGTMLLGMLMICSATEQLWVTVYYGVPVWRDAETTLFCASDAKAYDKEVHNVWATHACVPTDPDPKEINITNXTEXFNMWKNNMVEQMQIDIISLWDQFLKPCVKLTPLCVTLNCTAPTYNXTXXANSTSSTTGGGEMKNCSFNITTSIGNKVKDRAFFNELDIEPLNNSTDNTRYRLRHCNTTVITQACPKVSFEPIPIHYCTPAGFAILKCNNQTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEKEVVIRSSNFSDNAKVIIVQLKNLVKINCTRPNNNTRKGIHLGPGRAWYATDIIGDIRQAHCNLSGADWNNTLAEVSKKLREKFGNKTIIFNQTSGGDPEITMHSFNCRGEFFYCDTSRLFNSTWSNNGTTNGTTGNDTITLPCRIRQIVNMWQEVGRAMYAPPIAGQINCSSNITGLLLTRDGGNTNDTNNSTETFRPGGGDMRDNWRSELYKYKIVKIEPLGIAPTKAKRRVVQREKRAIGLGALFLGFLGAAGSTMGAASMTLTVQARLLLSGIVQQQNNLLRAIDAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLMGIWGCSGKLICTTTVPWNTSWSKRSLEEIWDNMTWMEWEREIDNYTKEIYTLIEESQNQQEKNEKELLELDKWANLWNWFDISNWLWYIKIFILIIGGLIGLRIVFAVLSIVNRVRQGYSPLSFQTRLPAPRGPERPEGIEE",
    "response#2": "MRVRGILRNWQQWWIWGILGFWMIYNVVGNLWVTVYYGVPVWKEAKTTLFCASDAKAYEREVHNVWATHACVPTDPNPQEMVLGNVTENFNMWKNDMVDQMHEDIISLWEQSLKPCVKLTPLCVTLICSNATADNATEMNGEIKNCSFNTTTEMRDKQQRAYALFHRPDIVPINESKKEYILIHCNTSTITQACPKVSFDPIPIHYCAPAGYAILKCNNKTFNGTGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIVRSENLTDNVKTIIVHLKKSVAIVCTRPDNKISMKRIKIGPGRAFVATKGIKGDIRQAYCNISRPDWNETLEGVKKKLKEHYPNKTIEFEPHSGGDLEITTHSFNCRGEFFYCNTTNLFNISMTNDTDTFITLPCRIKQIINMWQRVGRAMYAPPITGNITCNSSITGLLLTRDGGTTNGTENNNTEIFRPTGGNMKDNWRSELYKYKVVEIKPLGIAPTGAKRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGLWGCSGKLICTTNVPWNSSWSKKNLTDIWDNMTWMQWEREIDNYTDTIYRLLEVSQTQQEQNEKDLLALDSWKNLWSWFSISKWLWYIKIFIMIVGGLIGLRIIFAVISIVRRVRQGYSPLSFQTLIPNPRGPDRLGGIEEEGGEQDRDRSVRLVSGFLALAWDDLRSLCLFS",
    "response#3": "LWVTVYYGVPVWKEAETTLFCASDAKSYVTEKHNVWATHACVPTDPNPQEIVLENVTENFNMWKNSMVEQMHQDIISLWDQSLKPCVMLTPLCVTLKCSNISRKTNNSGTIETEIQNEIKNCSFNMTTELRDKHQKVHALFYRLDIVPINDNETNLNNSEYRLINCNTSAIKQACPKVTFEPVPIHYCAPAGFAILKCRNKKFNGTGTCNNVSSVQCTHGIKPVVSTQLLLNGSLAEENIIIRSENITDNTKVIIVQLVEPVMINCTRPNNNTRTGVRIGPGQTFYATGDIIGDIRQAQCNINGTKWNETLEKVAGELRKHFNNKTIVFRPSSGGDLEVTTHTFNCGGEFFYCNTSSLFNSTWDENNTKSAQGPNGTANITIPCKIRQIVRMWQRVGQAMYAPPIPGVINCISNITGLILIRDGGNESATNETFRPIGGDMKNNWRSELYKYKVVKIEPLGVAPTRARRRVVQREKRXLGXFMXGWGXVQARQLLSGIVQQQRNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICPTAAPWNSSWSNKSLSEIWDNMTWMEWEREIGNYTKQIYTLIEETQNQQEKRXLLXWVXPTQXXGXHXSXHXPXHXHXAXVQARQLLSGIVQQQRNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICPTAAPWNSSWSNKSLSEIWDNMTWMEWEREIGNYTKQIYTLIEETQNQQEK"
  },
  {
    "reference": "MYIKYLKLINFRNYKELDIEFDKNINIFVGDNAQGKTNILESMYYCSIGKSPRTSKDKELINWDNKESYIKVHILKKLFNKKIEIKIFKEGKKGININSIKVSKLSELMGVLNVVMFSPEDLKIIKESPVYRRKFLDIELCKFSKKYYYGLVQYNKVLTARNIILKKWNKGNYIDILQVYDKQLAKYGEVIIKLRNNYLKKLSEKGKVIHSDITSGIENIEFKYMTCLTNFDNIEDDLFKILEFNRKKDIYKGITLYGPHRDDFIVNINGVNVRNFGSQGQQRTSILTMKFASLEIIKEIIGEYPVLLLDDVLSELDKNRQKYILSSIKDIQTFITCTGIDDIKKSIIDEAQLFIVKKGKVSRI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: It also seems to bind ATP.; RecF binds preferentially to single-stranded, linear DNA.2. The designed protein should be involved in DNA replication, DNA repair by localizing to cytoplasm and exhibiting single-stranded DNA binding, ATP binding. The designed protein sequence is ",
    "response#1": "LTPHMEGIFTSGSSDRRKFLG",
    "response#2": "LTPHMEGIFTSGSSDRRKFLG",
    "response#3": "ITPEGFTLLNGGPKYRRAFLG"
  },
  {
    "reference": "MADIKEGVEEGSVKVDMIKEPLTQGDTGVDEVDFAKITLDDAFKYLNCNKHGLSSAEAAARLQQHGPNKLPDSSRNPVLVFLGYMWNPLAWAMEAAAIISIALLDVADFVLIVGLLLINAIISFYEESNADKAIKALTAALAPKAMVVRDGAIVTIDAVNLVPGDVILIRLGNIVPADVKLLEEEGADEGEQEAPMQIDQAALTGESLPAKKFTGDVAFSGSSIKQGERHAVVYATGVNTFFGRAAALISGTNNVSNLQTVMNKMSAICIVTILLWVVVELAVQFGHYSHECVGGREGCPTLLNMLVVLVGGIPIAMPTVLSVTLALGAYKLAREGAIVTRMSAVEEMAGMDVLCSDKTGTLTLNKLSIDKSMVVPVGNMGVDEIMRMGALSANTVTEEPIDMVLWESYPDRETIKRDYKHTKYFPFNPNDKITIATCLEIATGRVFRVLKGSPQVVLAKAWNAAELDATVNQKMVEFANRGFRALGLAMADGDGKDGTKWEMLALLPLFDPPRHDTKETIEHCQNQGIQVKMITGDHLLIGKETAKMLGMGTEMFPSEVMIKARNGDASQLHGYKNFVEMVETCNGFAQVFPEHKFEIVKILQDSNHVVGMTGDGVNDAPALKKADVGVAVADATDAARGAADIVLTEPGLSTIVTAVIGARKIFQRMTTYSKYTIAMTFRICFTFGLITVIYDWYFPTILIVIMAVFNDGAMIALSKDRVVASKTPNSWNITNIFIMGMVYGLYLTLSTWALYQTATKTTFFEDKT",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should emphasize Basic and acidic residues, Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "ILSGVVTSMYYTRAKRAAMDSKKAFLSAEDFRNLDINLFTAGYFGLRRQLKVGNIIYEKFRDELARSKSPKIQWWGPIDFANYVLAPEVLVSFVLDTCQNKRSVQLTSRQDVYELFDNTTYYGNTVTDQ",
    "response#2": "ILSGVVTSMYYTRAKRAAMDSKKAFLSAEDFRNLDINLFTAGYFGLRRQLKVGNIIYEKFRDELARSKSPKIQWWGPIDFANYVLAPEVLVSFVLDTCQNKRSVQLTSRQDVYELFDNTTYYGNTVTDE",
    "response#3": "ILSGVVTSMYYTRAKRAAMDSKKAFLSAEDFRNLDINLFTAGYFGLRRQLKVGNIIYEKFRDELARSKSPKIQWWGPIDFANYVLAPEVLVSFVLDTCQNKRSVQLTSRQDVYELFDNTTYYGNTVTDQ"
  },
  {
    "reference": "WIMGHMVNEIYQIDEFVDLGANSIETDITFDENAVAEYSYHGVPCDCRRWCHKWEYVNDFLNALRRATTPGDSKYRRELVLVVFDLKTGDLSSSTANKGGKLFAQKLLQHYWNGGNNGGRAYIIISIPDIDHYAFISGFRNALKEAGHEELLEKVGYDFSGNDDLNSIRNALHKAGVKDKEHVWQSDGITNCILRGLSRVREAVRNRDSSNGYINKVYYWTIEKYVSVRDALDAGVDGIMTNEPDVIVNVLNEKAYKQRFRLANYDDNPWETYQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.2. The protein can be targeted to extracellular region and perform lyase activity, metal ion binding, phosphoric diester hydrolase activity, toxin activity.3. The protein must exhibit the following characteristics: It may also act on ceramide phosphoethanolamine (CPE), lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE), but not on lysophosphatidylserine (LPS), and lysophosphatidylglycerol (LPG) ; This toxin acts on sphingomyelin (SM) ; It acts by transphosphatidylation, releasing exclusively cyclic phosphate products as second products ; Induces dermonecrosis, hemolysis, increased vascular permeability, edema, inflammatory response, and platelet aggregation ..4. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.5. The protein's active site, Nucleophile must be carefully engineered to ensure that the amino acids involved in a 1-acyl-sn-glycero-3-phosphoethanolamine = a 1-acyl-sn-   glycero-2,3-cyclic phosphate + ethanolamine are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "MSEEKKFITPTSFTRKLTRRGRAASSGSDTSGSESSPVSVPEKKKKKVSRPNSLKSNGQPKKKITRQNSLSFKDIKKKKTQNTKQNATPSKQSNQEEEESFVSEKKKTPKKRKKRTSPLESDEDSDLDYEPLPKKPKKKAKRTPPPSSSSSEEEEEDEEEEEPEKPKKKQKTEKKEKKEAKSKEQEKDKPKEKKGQEKKEKKGKEKKDKDAKKKEESKAKKGEKSSKEKDSSSSEESSSSESESESEKEESKKKKKAPPAKKAKKPASSSSSSESSSSEEEEEEEVKKKKKPAPAAAKKAKKASSSSSSEESSSSEEEEEEEVKKKKKPAPAAAKKAKKASSSSSSEESSSSEEEEEEKEKKKKGAPPVKKAKTPSSSDSSSSEEEEEEKKKKGAPPAKKAKKASSSSSSESSSSEEEEEEEEEVKKKKGAPPAKKAKKASSSSSSESSSSEEEEEKKKKKNGAPPAKKAKKASSSSSSESSSSEEEEEEKKKKGAPPAKKAKKASSSSSTEESSSSEEEEEEKKKKGAPPAKKAKKASSSSSSEESSSSEEEEEEKKKKGAPPAKKAKKASSSSSSESSSSEEEEEEKKKKGTPPAKKAKKASSSSSSEESSSSEEEEEEKKKKKKGAPPAKKAKKASSSSSSESSSSEEEEEEKKKKGAPPAKKAKKASSSSSSSGSSSSEEEEEEKKKKGTPPAKKAKKASSSSSSEESSSSEEEEEEKKKKGAPPAKKAKKASSSSSSSSSSSSEESSSSEEEEEEKKKKGAPP",
    "response#2": "NLYQFKNMIQCANHGRRATWHYLDYGCYCGPGGLGTPVDELDRCCKTHDDCYIEAGKKGCFS",
    "response#3": "MGARGPRNLVQFSYVITCANHNRRSSLDYADYGCYCGAGGSGTPVDELDRCCKIHDDCYGEAEKQGCYPKMLMYDYYCGSNGPYCRNVKKKCNRKVCDCDVAAAECFARNAYNNANYNIDTKKRCS"
  },
  {
    "reference": "MQKARPSKGTPKAAGGARQSGQFRVPQLTAQQQLRAQQQQEEEDAAVDALDDALIFDDEEGGTRIGDIYIPPPVPPHCSMESTGPRLIISKIVNRNFKSYAGEVELGPFHQSFTAIIGPNGSGKSNVIDSMMFVFGCRANRIRCKRVSTLIHSSSSYPNLRSCSVAVHFKQIVDKGDGTCEDVPDSSIVIERTAMSDNSSYYQINDKRAQLKDVAKLLKKHHVDLEHNRFLILQGEVESIAMMKPKGQTENETGMLEYLEDIVGTQRYIRPLQQINQRVDQLTDDRTEKHNRCKLAEREMKDLEQPFNEAVDYLKKENELVRTKSFHIQKIISIKKSKLEQYTQEHEACAEELKTHDEGTAALKQSRAEKETIIRKEIEEYEALVKKREQIKKRLVTVESAYTEIQSTMENTNKQRKKDKAQIEKNEKELEDLHKLPEKNQREIEDCNKKLESLEVSKVTLNEELEKQQAELTKTTAPLTEKRLKLSDELVGLKEKVNTAKGEVQVFESQLKILKQAETTESRKYETLKSSYEQSQKSLEEKVTRVDELKESIPRMKTEIASKSAEVDKMVKEERNLSMQCNKLRTEINERSSVMQAQRSNNKVLDFLMRMKMEGKIPGILGRLGDLGGIDAKYDIAISTACGRLDNIVTDNYETASAAIGALKEYNVGRATFITLDKIEHHRREANSRINTPENVPRLYDLVKVEDDRVRTAFYFALRNTLVCDDLEQGTRIAYGRERYRVVTLRGEMIEMTGTMSGGGSRPIRGKM",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in chromatin, cytoplasm, condensin complex that is critical for mitotic cell cycle. The designed protein sequence is ",
    "response#1": "FYGVTLTAESDSVTWDVDEDYARGQKLVIKQILLGAEAKENEFNVVEVNTPKDSVQIPIAVLKAGETRAVNPDVEFYESKVTFKLIKGSGPVYIHGHNR",
    "response#2": "FYGVTLTAESDSVTWDVDEDYARGQKLVIKQILLGAEAKENEFNVVEVNTPKDSVQIPIAVLKAGETRAVNPDVEFYESKVTFKLIKGSGPVYIHGHNC",
    "response#3": "FYGVTLTAESDSVTWDVDEDYARGQKLVIKQILLGAEAKENEFNVVEVNTPKDSVQIPIAVLKAGETRAVNPDVEFYESKVTFKLIKGSGPVYIHGHNF"
  },
  {
    "reference": "MARSDANSLKVFAGRRHIDLTQRICRRLDLPLGQAATSEFPDGELIVKTQEDVRGRDCYIVLSTCQPVNENLMELLIFIDCLKRASAARVTAVIPYFGYARQDRKDEGRTPITAKLVANIITTAGADRVLTLDLHAAQIQGFFDILVDHMSAAPVLLEYFESIREELGDLVVVSPDVGNVKIATLYAGLLGGDIAIIDKRRRTGSEVVSANLVGDVEGRSVLMIDDMISTAGTICSAAEVVMDRGATQVIAAATHPVLVGLAMERLAASPIKRVVVTNTIPMDGRCEPIRDKLVELCVGPLLGDAIHRIHHNQSVSALFRKGSGSKR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The D-ribose 5-phosphate binding site should be located in a region of the protein that is accessible to the ligand.2. The protein should be designed to catalyze the following reaction ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-   diphosphate + AMP + H(+).3. For general function, the protein need meet that Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P).4. Mg(2+) is required for the proper folding and stability of the protein.5. The designed protein should have magnesium ion binding, ribose phosphate diphosphokinase activity within cytoplasm to regulate ribonucleoside monophosphate biosynthetic process, 5-phosphoribose 1-diphosphate biosynthetic process. The designed protein sequence is ",
    "response#1": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGADRLMSVDLHAPQIQGFFNVPVDHLAPVLVRDIQARLKLDNVMVVSPDVGGVVRARALAKRIDAPLAIVDKRRERAGESEVMNIIGDVDGKICILIDDIVDSGGTLCNAADALRAQGASDVYAYITHGVLSGGAAAAAPVMYQYLKQMQVGDLVVVSPDVGGLKMAYAYSQVLSAGLAIVAKRRKSATEVESLTVIGEIRGKNVLMVDDLTETAGTLTTAAALLKRKGAKRILACVSHVLLNDLGVERLRKSSIDELITTDTIPLSEEARHFAQLELEDLTVVSADEGFAKKARRLADRLNAPLAIIEKRRLGNNGRIEAMGIIGNIEGRHALVVDDEIDTAGSLTQAVRVVHEHGARDIYCCATHGVFSGPAIERLSRAPIKEIVITDTIPLGERAERVKVISVAPLIARAILEVFDDGSVTSMFDGAA",
    "response#2": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGADRVISMDFHAAQIQGFFNIPVDHLFAAPVLVGYFNNLGLPDLTVVAPDAGGVERARAYAKRLNAELAIIDKRRDYERLGEVEVLHVVGDVEGRTTLIVDDMIDTAGTLVKAGEALKRSGAARVLASSTHALFSGPAVERIEASPIDQVVVTNTIPLSEKAQACTKVRQLSIAQLIADAIIRVHNRQSVSVLFR",
    "response#3": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGADRIMSVDLHAAQIQGFFNKPVDSIFTGNANRQLAGAICRELRIPLGDADVFQFSNENIFVKINENVRGRDVFVVQPFSAPVNRSIMELLIMIDALKRASSARITAVIPYYAYGRTDKKDQPRVPITARLIADCITVAASPIMIPYLRDHLKINGEAVIVAPDSGSVKLAHSFADRLNCGFAVVAKRRVSATTVESSHLVGDVKGRDCVICDDLTSTAGTLCAAAELLKKEGAKKVYAVVSHCLLTEAAQEKLKNSAIEELVTTNTIPLSEAAKGCSKIKELSIAGLLGRAIESIHEETSVSRLFD"
  },
  {
    "reference": "MDKPLHIVILAAGEGTRMKSSLPKMLQTVGGKPMLAHLLDTAVTLEPERVHVVVGSGAEQVRESCSAYPVTWALQAERRGTGHAVMQAMPGIPDEATVLVLLGDHPLIPASLLSDMLAERSQPLVLLTMNLDDPHGYGRILRDPGGNIIGVIEHHEAAPEQHKIREVNTGIIMADAAKLRGWLDRLSCDNAKNEYYLPDIFSMARTENGEVHGVLAPDERDLRGANDHGQLAELEKRYRQRRAMELMEAGVHVLDPERVDIRGSVTTGLDVCIDINVVLEGENHLGDGVRIGPGSVLTNCRLAAGGIVHPHSVLEGVTTTGPCEIGPFARLRPGTVLSAGCRVGNFVETKNARLDEGTKASHLSYLGDSEIGKNVNIGAGTITCNYDGANKHETVIEDDVFIGSDTQLVAPVTVGRGATIGAGSTVTKDAPPDALTVSRARQASVSNWKRPRKEKDK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc) The designed protein sequence is ",
    "response#1": "GVTLADPARVDVRGTLRCGRDVSIDVNCVFEGNVTIADNVTIGANCVIRNASVGAGTRIDAFTHIDGAELGANTVIGPYARLRPGAQLADEAHVGNFVEVKNAVIGHGSKANHLTYIGDADIGARVNIGAGTITCNYDGANKFRTVIEDDVFVGSDTQLVAPVRVGRGVTIAAGTTIWKDVAEGVLALNEKTG",
    "response#2": "VVVLAAGAGTRMKSDLQKTLHSIGGRSLISHSLHAAAGLNPTHIVAVIGHGRDQVGPAVEDVATHLGREVRIAIQEEQNGTGHAVQCAMDQLEGFDGTIIVTNGDVPLLTSDTLGSLLAAHTATPTAVTVLS",
    "response#3": "GATIVDPATTWIDVEVTIGRDVLINPGTQLRGTTSIGDRAEIGPDTTLTNMVIGTGASVIRTHGSDSEIGEDATVGPFTYIRPGTKLGAEGKLGGFVETKKATIGRGSKVPHLTYVGDATIGEYSNIGASSVFVNYDGVNKNHTTIGSHVRTGSDTMFIAPVTVGDGAYSGAGTVIKDDVPPGALAVSGGRR"
  },
  {
    "reference": "MTEAEDFAQLLQKLQNASPALPEGTSSTPTPSSSTGLLPNGKKTKGRVKIKMEYINNKLRRYTTFSKRKTGIMKKAFELSTLTGTQVMLLVASETGHVYTYATPKLQPMISSDTGKAMIQSCLNAPGGDGSDVQPSRTEFTFDSGNGGNGMRKRKMLSDVMSAESSNNSPSMPNFTPFLAPSMAPLFSTFGEDDYNNDESGDDSDSEEASSDIKEEYQGSPTMVKQETTEIDSVTASLQQRIKEAMRQAASNKQAIKKAKITPPSSNMNNAAFLNPFLLQGGANASNLLATAARKGDDAGSLTSNPFLSMGLNLQQLIESAAIASNE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must have at least one MADS-box domain.2. The designed protein should have protein dimerization activity within nucleus to regulate regulation of transcription by RNA polymerase II.3. Target a Acidic residues compositional bias in the protein's composition for improved properties.4. For general function, the protein need meet that Regulates myogenesis, in cooperation with transcription factors hlh-1 and hnd-1 The designed protein sequence is ",
    "response#1": "MGRGRSEIKRIENPTQRQSTFYKRRDGLFKKARELAVLCDADLLLLLFSASGKLYHFLSPN",
    "response#2": "RQERRAELEKVGSMCDLLEKQLRFMTVDDGEEYTVPSLEALEHNLEAAMRKVRSEKDRKIGGEICYLQNIIRGRQEERYGLCDKIAHAQTC",
    "response#3": "MGRRKIEIQRISDDRNRAVTFIKRKAGLFKKAHELSVLCQVDIAVIILGSNNTFYEFSSR"
  },
  {
    "reference": "MDRLLRLGGGMPGLGQGPPTDAPAVDTAEQVYISSLALLKMLKHGRAGVPMEVMGLMLGEFVDDYTVRVIDVFAMPQSGTGVSVEAVDPVFQAKMLDMLKQTGRPEMVVGWYHSHPGFGCWLSGVDINTQQSFEALSERAVAVVVDPIQSVKGKVVIDAFRLINANMMVLGHEPRQTTSNLGHLNKPSIQALIHGLNRHYYSITINYRKNELEQKMLLNLHKKSWMEGLTLQDYSEHCKHNESVVKEMLELAKNYNKAVEEEDKMTPEQLAIKNVGKQDPKRHLEEHVDVLMTSNIVQCLAAMLDTVVFK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Also involved in homologous recombination repair by promoting RAD51 loading.; The PSMD14 subunit is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the complex; Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins; Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair; This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required; Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation2. The designed protein should contain at least one JAMM motif motif to achieve its intended function.3. The presence of MPN domains is critical for the interaction of the designed protein with its intended targets.4. The designed protein should have a binding site that is specific to the target Zn(2+) ligand. The designed protein sequence is ",
    "response#1": "LCCKQCQDTEITSKNEIFSLSLYGPMAAYVNPHGYVHETLTVYKASNLNLIGRPSTLHSWFPGYAWTIAQCRTCSSHMGWKFSAVKKDLSPPRFWGLTRSAGMVTIGRGFSVTYQLISKVCPLMISRSHCVLKQNPEGQWTIMDNKSLNGVWLNRERLAPLQSFCIRKGDHIQLSGRHYWEVEVGDKTHWAVGVCRDSVSRKGELTPLPETGYWRVRLWNGDKYAATTTPFTPLHIKVKPKRVGIFLDYEAGTLSFYNVTDRSHIYTFTDTFTEKLWPLFYPGIRAGRKNAAPLTIRPPTRHYWEVEVGDKTHWAVGVCRDSVSRKGELTPLPETGYWRVRLWNGDKYAATTTPFTPLHIKVKPKRVGIFLDYEAGTLSFYNVTDRSHIYTFTDTFTEKLWPLFYPTRFTFYPCVLATEGFTSGRHYWEVEGALCCKQCQDTEITSKNEIFSLSLYGPMAAYVNPHGYVHETLTVYKASNLNLIGRPSTLHSWFPGYAWTIAQCRTCSSHMGWKFSAVKKDLSPPRFWGLTRSALLPA",
    "response#2": "LCCKQCQDTEITSKNEIFSLSLYGPMAAYVNPHGYVHETLTVYKASNLNLIGRPSTLHSWFPGYAWTIAQCRTCSSHMGWKFSAVKKDLSPPRFWGLTRSAEVTIGRGFSVTYQLISKVCPLMISRSHCVLKQNPEGQWTIMDNKSLNGVWLNRERVKRHYWEVEVGDKTHWAVGVCRDSVSRKGELTPLPETGYWRVRLWNGDKYAATTTPFTPLHIKVKPKRVGIFLDYEAGTLSFYNVTDRSHIYTFTDTFTEKLWPLFYPDLIADVTLDPETAHPNLVLSEDRKSVKFVETRLRDLPDTPQRFTFYPCVLATEGFTSGRHYWEVEVGDKTHWAVGVCRDSVSRKGELTPLPETGYWRVRLWNGDKYAATTTPFTPLHIKVKPKRVGIFLDYEAGTLSFYNVTDRSHIYTFTDTFTEKLWPLFYPGIRAGRKNAAPLTIRR",
    "response#3": "VRISAVALIKMVMHARSGGIYEIMGLMQGKIDVENRTLYVMDSFALPVEGTETRVNAQNEAYEYMVQYLDHSKEVGRLENVVGWYHSHPGYGCWLSGIDVNTQRTNQQFQDPFVAIVIDPNRTISSGKVDIGAFRTFPELCCKQCQDTEITSKNEIFSLSLYGPMAAYVNPHGYVHETLTVYKASNLNLIGRPSTLHSWFPGYAWTIAQCRTCSSHMGWKFSAVKKDLSPPRFWGLTRSAEVTIGRGLGLTYQLVSKICPLMISRNHCVFKQNEEGQWTVKDNKSLNGVWLNKERVGLCCKQCQDTEITSKNEIFSLSLYGPMAAYVNPHGYVHETLTVYKASNLNLIGRPSTLHSWFPGYAWTIAQCRTCSSHMGWKFSAVKKDLSPPRFWGLTRSACLESAVELPKILCQEEQDAYRRIHSLTHLDSVTKIHNGSEVFTKNLCSQMSAVSGPLLQWLEDRLEQNQHHLQELEQEKEEKLIADVTLDPETAHPNLVLSEDRKSVKFVETRLRDLPDTPQRFTFYPCVLATEGFTSGRHYWEVEVGDKTHWAVGVCRDSVSRKGELTPLPETGYWRVRLWNGDKYAATTTPFTPLHIKVKPKRVGIFLDYEAGTLSFYNVTDRSHIYTFTDTFTEKLWPLFYPGIRAGRKNAAPLTIRVHI"
  },
  {
    "reference": "MVNSNQNQNGNSNGHDDDFPQDSITEPEHMRKLFIGGLDYRTTDENLKAHFEKWGNIVDVVVMKDPRTKRSRGFGFITYSHSSMIDEAQKSRPHKIDGRVVEPKRAVPRQDIDSPNAGATVKKLFVGALKDDHDEQSIRDYFQHFGNIVDINIVIDKETGKKRGFAFVEFDDYDPVDKVVLQKQHQLNGKMVDVKKALPKQNDQQGGGGGRGGPGGRAGGNRGNMGGGNYGNQNGGGNWNNGGNNWGNNRGGNDNWGNNSFGGGGGGGGGYGGGNNSWGNNNPWDNGNGGGNFGGGGNNWNNGGNDFGGYQQNYGGGPQRGGGNFNNNRMQPYQGGGGFKAGGGNQGNYGGNNQGFNNGGNNRRY",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein can be expressed in cytoplasm, ribonucleoprotein complex, nucleus and perform sequence-specific DNA binding, poly(G) binding, mRNA binding, mRNA 3'-UTR binding without triggering undesirable cellular responses.2. Target a Polar residues compositional bias in the protein's composition for improved properties.3. The designed protein have RRM domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "KRVEVKPYVLDDQLCDECQGTRCGGKFAPFFCANVTCLQYYCEYCWAAIHSRAGREFHKPLVS",
    "response#2": "KRVEVKPYVLDDQLCDECQGTRCGGKFAPFFCANVTCLQYYCEYCWAAIHSRAGREFHKPLVN",
    "response#3": "KRVEVKPYVLDDQLCDECQGTRCGGKFAPFFCANVTCLQYYCEYCWAAIHSRAGREFHKPLVV"
  },
  {
    "reference": "MIPELEDLSLDEPILIIGDLMLDEYFLGDVERISPEAPVPVVRAQQHERRVGGAGSVVANLSALGIPTKVLGLVGRDEAGDHIVSALSQLGADVEEVVTSDQRPTSCKTRILAGVQQAGRGQQQILRLDREEVQEISVEETLACRSALSRIFAGPLSMILVSDYEKGLLSEDLIQFVITEAKQRGIPVLVDPGRSGQWSKFTGATLICPNRFETSAATGMEVGAEVPEHAQAAESLIDRLKLEHCILTLDRDGITRVSKGGELRRHPTRVQAVTDVAGAGDMVLSLLGVALASGWSMDEACQLSNLGAGLEIQKVGVQPVERWELSAARDAHCGIQPDSARSASEMVRIVENYRTAEQKVVFTNGCFDLLHSGHLQLLEAARAFGDALVIGLNSDASVTRLKGAERPILPASERARLLGALSCVDHVVIFEEDTPEQLIDALLPDVLVKGADYAIEEVVGREAVEAAGGRVETVTLRPGFSTTGLIEQIRSGTRSCPQTPEG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must be able to heptose 7-phosphate kinase activity.2. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7- phosphate at the C-1 position to selectively form D-glycero-beta-D- manno-heptose-1,7-bisphosphate..3. Ideal protein should have a binding affinity for key enzymes in the Nucleotide-sugar biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MPGGAGNVVRNLIALNASAAFVSVVGDDAAGSDLTGLIGGQERVEPWLLVQGGRTTTLKTRYIAQGQHLIRADREETVALPARLAERLVRIALDAMAATTVTILSDYRKGVLSPEVAGQLIAGAKKLGRQVIVDPKGRDYALYAGADVITPNRRELAEATGMPVDSEAGIVAAARKLMAAHGFGAVLVTRSEDGMSLVMQGEVHHFPGEAKEVFDVSGAGDTVVATLAAALAANVPLPEAARLANIAGGIVVGKVGTAVRVLVAGDLMLDRYWDGSTVRISPEAPVPVVRVGTETERPGGAGNVALGIAALGGHATVVGLCGADAPGERLRGLLEEAGVAVRAVVLEGCATVTKLRVLSRHQQLIRLDFEDEEPALDTARVLEEFRAALADADLVVFSDYGKGVLRDAPALIAAARAAGRAVLVDPKGGDFERYRGASLVTPNRTEFEAVAGVCRGDAELEARARTLAGRHGLDALLVTLGEEGMMLVPAAGPALRLPAQAREVFDVTGAGDTVIGVLAAALAAGEDLAGATALANLAAGIVVGKLGAATVTAPELREVLHAL",
    "response#2": "MPGGAGNVVRNLIALNASAAFVSVVGDDAAGSDLTGLIGGQERVEPWLLVQGGRTTTLKTRYIAQGQHLIRADREETVALPARLAERLVRIALDAMAATTVTILSDYRKGVLSPEVAGQLIAGAKKLGRQVIVDPKGRDYALYAGADVITPNRRELAEATGMPVDSEAGIVAAARKLMAAHGFGAVLVTRSEDGMSLVMQGEVHHFPGEAKEVFDVSGAGDTVVATLAAALAANVPLPEAARLANIAGGIVVGKVGTAVS",
    "response#3": "MPGGAGNVVRNLIALNASAAFVSVVGDDAAGSDLTGLIGGQERVEPWLLVQGGRTTTLKTRYIAQGQHLIRADREETVALPARLAERLVRIALDAMAATTVTILSDYRKGVLSPEVAGQLIAGAKKLGRQVIVDPKGRDYALYAGADVITPNRRELAEATGMPVDSEAGIVAAARKLMAAHGFGAVLVTRSEDGMSLVMQGEVHHFPGEAKEVFDVSGAGDTVVATLAAALAANVPLPEAARLANIAGGIVVGKVGTAVV"
  },
  {
    "reference": "MIRTMFKSKIHRATVTHANLHYVGSVTVDKDLLDAADILPGELVAIVDVTNGSRLETYTIEGQAGSGVIGINGAAAHLVNIGDTVILISYAQMTTAEAREYLPSIVHVDAANKIVQLGTDPAEALTEGLNRPPFSTTA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must have a Schiff-base intermediate with substrate; via pyruvic acid, Proton donor that is structurally stable and resistant to denaturation.2. The designed protein should interact with enzymes and substrates within the beta- alanine from L-aspartate: step 1/1, Cofactor biosynthesis pathway to enhance or regulate its activity.3. The protein must bind to pyruvate in order to perform its enzymatic function.4. The substrate binding site should be stable and able to withstand changes in environmental conditions.5. The protein should have a specific function in pantothenate biosynthetic process, alanine biosynthetic process and should be localized to cytoplasm in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "MACGETIVIATHNPGKVRELAALLAPHGVTLRSAAEAGLPEPEETGTTFAENALLKARAAALATGLPALADDSGLAVDVLGGAPGIFSARWAGRHGDDEANLDLLLAQLADVPPERRTARFHCTLVAVRSADDPEPIVVRGEWHGELLTAPRGEGGFGYDPLFLVDGRDRTAAQLAPEEKNAVSHRGKAFRALAELVPAIL",
    "response#2": "MKIVEV",
    "response#3": "MPLTIDDFKAAVQEK"
  },
  {
    "reference": "MESLTLQPIARVDGTVNLPGSKSVSNRALLLAALARGTTVLTNLLDSDDVRHMLNALSALGVHYVLSSDRTRCEVTGTGGPLQAGSALELFLGNAGTAMRPLAAALCLGSNDIVLTGEPRMKERPIGHLVDALRQGGAQIDYLEQENYPPLRLRGGFTGGDVEVDGSVSSQFLTALLMASPLAPQDTVIAIKGELVSRPYIDITLHLMKTFGVEVENQAYQRFIVRGNQQYQSPGDYLVEGDASSASYFLAAGAIKGGTVKVTGIGRNSVQGDIRFADVLEKMGATVTWGEDYIACTRGELNAIDMDMNHIPDAAMTIATAALFARGTTTLRNIYNWRVKETDRLFAMATELRKVGAEVEEGEDYIRITPPLTLQFAEIGTYNDHRMAMCFSLVALSDTPVTILDPKCTAKTFPDYFGQLARISTLA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must have a Proton acceptor, Proton donor that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "RIDFKEGKLSPEWIHLQNPEAKNYIFTKDGKLRLIATPVTLSDWKSPTFVALRQEHFDMEASAPVVLQKAGVNDEAGISVFMEFHSHYDLFVRQDKDRKRSVGLRYKLGEITHYAKEVSLPTDGEVELVVKSDINYYYFGYKVNGIYHDLGKMNTRYLSTETAGGFTGVVLGLYITSASKDSKAYADFEYFKYN",
    "response#2": "RIDFKEGKLSPEWIHLQNPEAKNYIFTKDGKLRLIATPVTLSDWKSPTFVALRQEHFDMEASAPVVLQKAGVNDEAGISVFMEFHSHYDLFVRQDKDRKRSVGLRYKLGEITHYAKEVSLPTDGEVELVVKSDINYYYFGYKVNGIYHDLGKMNTRYLSTETAGGFTGVVLGLYITSASKDSKAYADFEYFKYE",
    "response#3": "RIDFKEGKLSPEWIHLQNPEAKNYIFTKDGKLRLIATPVTLSDWKSPTFVALRQEHFDMEASAPVVLQKAGVNDEAGISVFMEFHSHYDLFVRQDKDRKRSVGLRYKLGEITHYAKEVSLPTDGEVELVVKSDINYYYFGYKVNGIYHDLGKMNTRYLSTETAGGFTGVVLGLYITSASKDSKAYADFEYFKYN"
  },
  {
    "reference": "MKVLYFDCFSGISGDMILGALLDLGVNLEEWKNELNKIPVEGYKIEISKKQKNHIWGTDVEIIIEDHHSHRHLEDLLEIVDKSELSENIKNRAKEVFYKIAEAEAKIHNTSINEVHFHEVGALDTVIDVLGSLILLDMLKVDKIYSSPLPLGGGFVNASHGTIPVPAPATLEILRGVPVYRDGREGELVTPTGAAIISTIAEFVKILPPIKVDKVGYGCGKKDFPFPNLLRVYEGELIEKVIEEKNVVLETNIDDMNPQIFGYLTEKLFKEGALDVFLTPVYMKKGRPGVLLTVIAPLAMEEKLLEVIFGETTTLGVRRIYVDKRIMQREIREIETKWGKVRIKVANINGIKKAYPEYEDCKSIAERENIPLKDVIGEIEKIIERELEV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be optimized for lyase activity, nickel cation binding. The designed protein sequence is ",
    "response#1": "KIICVGLNYIDHAKELNMEIPEYPIIFLKPTSAIIYNEDYIIRPRISKRVDYEVELAIVIGKKCKNIKKDEANDYIMGYTILNDVTARDLQQKDGQWTRAKSFDTFCPIGPRIVKDIDPMNLNIECRVNGEIKQKSNTKNMIFDVYELVEFVASIMTLYPGDIISTGTPPGVGELKAGDVVECEIEGIGILRNYL",
    "response#2": "KIICVGLNYIDHAKELNMEIPEYPIIFLKPTSAIIYNEDYIIRPRISKRVDYEVELAIVIGKKCKNIKKDEANDYIMGYTILNDVTARDLQQKDGQWTRAKSFDTFCPIGPRIVKDIDPMNLNIECRVNGEIKQKSNTKNMIFDVYELVEFVASIMTLYPGDIISTGTPPGVGELKAGDVVECEIEGIGILRNYMD",
    "response#3": "KIICVGLNYIDHAKELNMEIPEYPIIFLKPTSAIIYNEDYIIRPRISKRVDYEVELAIVIGKKCKNIKKDEANDYIMGYTILNDVTARDLQQKDGQWTRAKSFDTFCPIGPRIVKDIDPMNLNIECRVNGEIKQKSNTKNMIFDVYELVEFVASIMTLYPGDIISTGTPPGVGELKAGDVVECEIEGIGILRNYL"
  },
  {
    "reference": "METVLTARDLTRHYEVSRGLFKGHAQVRALNGVSFELEAGKTLAVVGESGCGKSTLARALTLIEEPTSGSLKIAGQEVKGASKDQRRQLRRDVQMVFQNPYASLNPRQKIGDQLAEPLLINTALSREERREKVQQMMRQVGLRPEHYQRYPHMFSGGQRQRIALARAMMLQPKVLVADEPTSALDVSIQAQVLNLFMDLQQQFRTAYVFISHNLAVVRHVADDVLVMYLGRPAEMGPADKLYENPLHPYTRALLSATPAIHPDPTKPKIRIQGELPNPLHPPEGCAFHKRCPYATERCRSEVPELRLLDQRQVACHHAEQFLG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a fast turnover rate for a dipeptide(out) + ATP + H2O = a dipeptide(in) + ADP + H(+) +   phosphate.2. The ATP binding site should be stable and able to withstand changes in environmental conditions.3. For general function, the protein need meet that Responsible for energy coupling to the transport system (Probable).; Part of the ABC transporter DppABCDF involved in the uptake of various di/tripeptides4. The designed protein should contain one or more ABC transporter domains that are essential for its function.5. The designed protein should have ATP binding, ATP hydrolysis activity within ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing to regulate protein transport, peptide transport. The designed protein sequence is ",
    "response#1": "LVFDKVNRVYPDGTQAVKNVSVHLEKGEFCVLLGPSGAGKSTLMNMVNGLVEPSSGEIILDGGVLNKKNLQLIQRSVSMIHQQLYLIPRLSVLHNVLTGILPTANFWTSLVKSFPVKDQQRAFELLSEVGLEEKHLMRRASALSGGQQQRVAIARAFMANPKVVLADEPVASLDPAMSRSVLNSLKHAAQTNGATVLCTLHQIDYALEFADRIVALREGEVFFDGHPSDMDEDIQKRLYEIEG",
    "response#2": "LKDVSLDLYPNEITALIGPSGSGKSTLLRSINRMNDLNPEVTITGSIVYNGHNIYSPRTDTVDLRKEIGMVFQQPNPFPMSIYENVVYGLRLKGIRDKSILDHAVESSLKGASIWNEVKDRLHDSAVGLSGGQQQRVCIARVLATSPRIILLDEPTR",
    "response#3": "LEVPGLNLFYGAKQALFDVRMNIPKQRVTAFIGPSGCGKSTLLRCFNRMNDLVDGCRVEGEIRLDGHNIFAKGVDVAELRRRVGMVFQKPNPFPKSIYENVVYGLRIQGINKKRVLDEAVEWALKGAALWEEVKDRLHESALGLSGGQQQRLVIARTIAVEPEVLLLDEPCSALDPISTLKIEELIYELKSKFTIVIVTHNMQQAARVSDYTAFMYMGKLIEFGDTDTLFTNPAKKQTEDYITGRFG"
  },
  {
    "reference": "MNPGQKQEHDQYPLHDTQFVPQQMDRNSPFADPYPEDQPPPSGYDHQPLLRDNAPSYPPDPFGQPGGYPPQSTMYPPQPMGPPSPNMRYGEAPRRQPRRYKTTRRVKLTHGNLILDCPVPTPYLQAVPIKDTKEFTHMRYTAATCDPADFASQGYTLRQPILQRNTELFIVLTMYNEDEILFARTMHGVMKNIAHLCSRVRSNVWEGPKAWEKVVVCIVSDGRKKIHPRTLSLLATLGVYQDGVAKNVVGDKPVTAHIYEYTTQLSVDPEMKFKGADKGMPPCQILFCLKENNQKKINSHRWFFQAFGPVINPNVCVLIDVGTRPGKTSIYHLWKAFDISSNIAGACGEIRAMSGTAGVALLNPLVAAQNFEYKMSNILDKPLESVFGYISVLPGAFSAYRFTALQNDENGHGPLEKYFLGESQHGADADIFTANMYLAEDRILCYELVAKKKANWVLHYVSSSYGETDVPDSVPEFISQRRRWLNGSFFAGCYALFHWRKVWASDHSFVRKLMFMFEDLYNTYNLIFSWFALGNFYLTFYILTSALGAESLDPKPFSANVASILHTILNYIYILLIIVQFILALGNRPQGSKWAYFGSMTFFAILMVYMMFATIWITVVGVQDAVANADGSFTAMLGESTFRNIIISIVSTYAMYFIASFLFFDPWHMFTSFIQYIFLSPSYTNILNIYAFCNTHDVSWGTKGDNTVSTDLGVVKSKKDGSGDTTVEVEVPTEQKDINEAYEEACVELTRQVEPEVSHRDAKTKQED",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design must be able to perform chitin synthase activity in order to contribute to cell wall organization. The designed protein sequence is ",
    "response#1": "IAAYWGQNAGGDQQTLGDYCSSSPASIIILSFLDGFPNLSLNFANQCSGTFSSGLAHCSQIGSDIKSCQQQGKTILLSLGGATGNYGFSSDSEAVQFAGTLWNKFGGGKDSERPFDDAIVDGFDFDIENKDQTGYAALATQLRKYFSTGTKSYYLSAAPQCPYPDESVGDLMSQVDLDFAFIQFYNNYCSLNQQFNWNSWSNYARGKSIKLYLGLPGQ",
    "response#2": "SNIAVYYGQGVNQPRLAEFCAETSYDIINIGFINSFPEQNPLTGLPGSDFGNQCWADTFVVDGIASQLYSHCPNIAEDIPKCQAAGKKVFLSLGGATPTYWFDTIDASTKLADFLWGAFGPVTDAWTVADKPRPFGNAVVDGFDFDIEFFGSKGYANMIKRFRRRFGEVPDQTFYISAAPQCSIPDEQLSVAIKNAVIDFVWVQFYNTPGCSARDFVLGTKNGFNYDSWVEVIKAGANPNAKLYVGLPASGAAANLGYYLTPEEVKPLVKKYMDKYPETFGGVMLWEATQARNNQIDGVGYNEKIRS",
    "response#3": "IAAYWGQNAGGDQQTLGDYCSSSPASIIILSFLDGFPNLSLNFANQCSGTFSSGLAHCSQIGSDIKSCQQQGKTILLSLGGATGNYGFSSDSEAVQFAGTLWNKFGGGKDSERPFDDAIVDGFDFDIENKDQTGYAALATQLRKYFSTGTKSYYLSAAPQCPYPDESVGDLMSQVDLDFAFIQFYNNYCSLNQQFNWNSWSNYARGKSIKLYLGLPGQ"
  },
  {
    "reference": "MILFSPIGTADPITALGDGPMLHIVRHYRPIVVVLFLSAEIAAFENADRRYSAAITRLAPETDVRIVTYTNPSVHRFDLFVPVFRNHLVELSAEFPDRTILLNTSSGTPAMQAALVAINVFGIPRTTAVQVSTPARALSKPGDRESPDAYDLELMWDANDDNQPGAPNRCFEATSAALGALLERANLKQLIVSYDYSAAVTIAADSRLPDQVSNLIRGAMHRSRLEHLVAPKFFKDTAFTYDPANKVAEYISALALLAKREQWAEFARSATPAITIVLRAAVAKHLPEDRYLDDMGRVDRRKLEREPEIRCALKHPPKSPNAEWYLYTKDWLALLRQFAPDRVGALEVLGRFESRVRNTAAHEIVSISEDRITKDGGLLPEQLLKILARETGADLTLYDRLNDEIIRQIDMAPLG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design must be focused on enhancing the efficiency of defense response to virus.2. The protein must exhibit the following characteristics: This subunit is a single-strand-specific endoribonuclease (ssRNase) that is stimulated by cyclic oligoadenylates (cOA) produced by the Cas10 subunit of the Csm effector complex .; CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA); This CRISPR-Cas system protects bacteria against transformation with plasmids containing DNA homologous to its spacer regions; The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities (Probable); CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids; CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). The designed protein sequence is ",
    "response#1": "MEIDELTALGGLLHDIGKPVQRAGLYSGDHSTQGARFLRDLAENTGRAEYELLSLFSEFHHKGHMKNDELMIRRIKELSPERFGLTMEDVLNALWIVYEADNLASLGASTNSAAGQLDVRIVTYTNPSVHRFDLFVPVFRNHLVELSAEFPDRTILLNTSSGTPAMQAALVAINVFGIPRTTAVQVSTPARALSKPGDRESPDAYDLELMWDANDDNQPGAPNRRLGVLRADIDNLGTTFIKGIPEQYRSISRTATLSRQLSMFFKFELSNILKGARISVIYSGGDDLFLIGAWDDVISKALVLRKAFTRFSAGKLTFSAGIGMYPVKYPISKMASETGVLEDLAKRGEKNQVLYRLTHDVRIVTYTNPSVHRFDLFVPVFRNHLVELSAEFPDRTILLNTSSGTPAMQAALVAINVFGIPRTTAVQVSTPARALSKPGDRESPDAYDLELMWDANDDNQPGAPNRISGSKALKSLRSRSFYLETMLESLVDDLLSDLELSRANLLYTGGGHAYLLLPNTERARDVLASFEGEMKEWFIKIFKTDLSVAIAYKACTGEDELTALGGLLHDIGKPVQRAGLYSGDHSTQGARFLRDLAENTGRAEYELLSLFSEFHHKGHMKNDELMIRRIKELSPERFGLTMEDVLNALWIVYEADNLYLGALMLDADRMGEAFATGFRREGRDLATPSRLAALSRTLEVFFTTEVLTLLEEPRRYRERLGWDDLEAQRKEARYPLLYSVYSGGDDLFLLGPWDALLDFALDLERLYRLFTRHPRLTLSGGFLLVPPSLPVPELARLLGEAEKRAKAEGRER",
    "response#2": "MNPQLIEAIIGCLLHDIGKPVQRAALGYPGRHSAIGRAFMKKVWLRDSRNPSQFTDEVDEADIGVSDRRILDAISYHHSSALRTAAENGRLAADAPARRLGVMKGDVDRLGEFFSSMDSPSKLATASRFMDYFFKGYIGAIIEGKFGYIIGDVPSLRDWPEEPDIVVVYAGGDDFFIVGAWDQIFELAFRVRRAFNAYTGGKLTLSVGLGYFDERTPIYRMADVVSERLDTAKDEGRNRVFVVGARLGVLRLDVDNLGQAFTHGFMEQGNGKFNTISRTAAFSRMLSLFFRQHINYVLARPKLRPITGDDPARPREATIIYSGGDDVFVVGAWDDVIEFGIELRERFHEFTQGKLTVSAGIGMFPDKYPISVMAREVGDLEDAAKSLPGKNGVALFDARLGVLRLDVDNLGQAFTHGFMEQGNGKFNTISRTAAFSRMLSLFFRQHINYVLARPKLRPITGDDPARPREATIIYSGGDDVFVVGAWDDVIEFGIELRERFHEFTQGKLTVSAGIGMFPDKYPISVMAREVGDLEDAAKSLPGKNGVALFDVTCSRLGVLRADIDNLGTTFIKGIPEQYRSISRTATLSRQLSMFFKFELSNILKGARISVIYSGGDDLFLIGAWDDVISKALVLRKAFTRFSAGKLTFSAGIGMYPVKYPISKMASETGVLEDLAKRGEKNQVPRLGVLRADIDNLGTTFIKGIPEQYRSISRTATLSRQLSMFFKFELSNILKGARISVIYSGGDDLFLIGAWDDVISKALVLRKAFTRFSAGKLTFSAGIGMYPVKYPISKMASETGVLEDLAKRGEKNQVALWN",
    "response#3": "MEIDELTALGGLLHDIGKPVQRAGLYSGDHSTQGARFLRDLAENTGRAEYELLSLFSEFHHKGHMKNDELMIRRIKELSPERFGLTMEDVLNALWIVYEADNLASDVRIVTYTNPSVHRFDLFVPVFRNHLVELSAEFPDRTILLNTSSGTPAMQAALVAINVFGIPRTTAVQVSTPARALSKPGDRESPDAYDLELMWDANDDNQPGAPNRPRLGVLRADIDNLGTTFIKGIPEQYRSISRTATLSRQLSMFFKFELSNILKGARISVIYSGGDDLFLIGAWDDVISKALVLRKAFTRFSAGKLTFSAGIGMYPVKYPISKMASETGVLEDLAKRGEKNQVALWNRLGVLRADIDNLGTTFIKGIPEQYRSISRTATLSRQLSMFFKFELSNILKGARISVIYSGGDDLFLIGAWDDVISKALVLRKAFTRFSAGKLTFSAGIGMYPVKYPISKMASETGVLEDLAKRGEKNQVRLGVLRLDVDNLGQAFTHGFMEQGNGKFNTISRTAAFSRMLSLFFRQHINYVLARPKLRPITGDDPARPREATIIYSGGDDVFVVGAWDDVIEFGIELRERFHEFTQGKLTVSAGIGMFPDKYPISVMAREVGDLEDAAKSLPGKNGVRLGVLRADIDNLGTTFIKGIPEQYRSISRTATLSRQLSMFFKFELSNILKGARISVIYSGGDDLFLIGAWDDVISKALVLRKAFTRFSAGKLTFSAGIGMYPVKYPISKMASETGVLEDLAKRGEKNQVAAWEIVKNKYIPSIIRVMKTVGVKFTEEDADKFMKTLIILHDVGKCSEVYQKHLSNNEPLRGFRHELVSAYYAYNILKDMFKDETIAFIGALVVMMHH"
  },
  {
    "reference": "MASLISFSLLPKPKAVRSSISAPQTQTINTEKLEDKFGRKGIKFSESNNIPMVELKVRNGSSLKLSLSDAHVLSYKPKVYWKDEGFEEVLYTVDGDESRGGVGVVIVNGEEPKGGSSVISGCDWSVKDTDSDAIDALQIELSCTAGVLDITYIVSLYPVSMATALVVKNNGRKPVTLKPGIMSYLRFKKRSGAGIQGLKGCSYCPNPPLSSPFELLSPSEAMKAESSGWFGSEEGEKPGIWAVEDSVITLLEKKMSRIYGAPPAERLKAVYNTPPSKFETIDQGRGLFFRMIRIGFEEMYVGSPGSMWDKYGKQHYFVCTGPTSMLVPVDVASGETWRGAMVIEHDNL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient.2. The protein should be designed to localize specifically to chloroplast thylakoid membrane in order to perform its role in carbohydrate metabolic process more effectively. The designed protein sequence is ",
    "response#1": "RMYLLTFDGYLRIHFKKYSGNKKGSFYSISIWGQKELKPVNSNFLLSTVNNNEKVSFFSTKTSQIDGNVRPLMHSLSIHFSNLSKKDIFTYPHESDNTMLLPLLLLGLFTWFVGLIGIPFNQEEEGFDILSKWLIPPINLLHQNSSSPVDWYEFITNSILSVSISVFGIFTASLLYGSVYSSFKNMELINSVVKISPKRSLLDRIINVIYNWSCNRGYIDVFYARSLTMMIRGLAQLTHFFN",
    "response#2": "RMYLLTFDGYLRIHFKKYSGNKKGSFYSISIWGQKELKPVNSNFLLSTVNNNEKVSFFSTKTSQIDGNVRPLMHSLSIHFSNLSKKDIFTYPHESDNTMLLPLLLLGLFTWFVGLIGIPFNQEEEGFDILSKWLIPPINLLHQNSSSPVDWYEFITNSILSVSISVFGIFTASLLYGSVYSSFKNMELINSVVKISPKRSLLDRIINVIYNWSCNRGYIDVFYARSLTMMIRGLAQLTHFFK",
    "response#3": "RMYLLTFDGYLRIHFKKYSGNKKGSFYSISIWGQKELKPVNSNFLLSTVNNNEKVSFFSTKTSQIDGNVRPLMHSLSIHFSNLSKKDIFTYPHESDNTMLLPLLLLGLFTWFVGLIGIPFNQEEEGFDILSKWLIPPINLLHQNSSSPVDWYEFITNSILSVSISVFGIFTASLLYGSVYSSFKNMELINSVVKISPKRSLLDRIINVIYNWSCNRGYIDVFYARSLTMMIRGLAQLTHFFN"
  },
  {
    "reference": "MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDESLAVVRHWDHVEVRAKDESQRGAIIGQLTRTPGIHHILEVEDRAYTDVHDIFEQTLAANRERIEGKSFCVRVKRRGKQEFSSQDVERYVGGGLNQHVASARVQLDKPEVTVNLEIDNDRLTLVNARFEGLGGYPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWKRFGSSHRVRFVAINFEPVVGEILEKVDDGQMGVVLKRMMVRAASKMAERYGVQALVTGEALGQVSSQTLTNLRVIDNASDTLILRPLISHDKEHIIKIAREIGTEDFARTMPEYCGVISKSPTVKAVKSKIEAEEQNFDFAILDRVVEEASNVDIREIAEQTQEEVVEVETVASCAANDAVLDIRSADEQDASPLSVDGVEVKALPFYKLATQFADLDQSKTWLLYCERGVMSKLQALYLHEQGFTNVKVYRP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a high affinity for ATP.2. The designed protein should be involved in thiamine diphosphate biosynthetic process, thiazole biosynthetic process, tRNA thio-modification, thiamine biosynthetic process by localizing to cytoplasm and exhibiting tRNA U4 sulfurtransferase, CCA tRNA nucleotidyltransferase activity, tRNA binding, ATP binding.3. The Cysteine persulfide intermediate active site of the designed protein must be optimized for substrate binding and specificity.4. The designed protein have Rhodanese, THUMP domains with specific sequence motifs that are crucial for its activity.5. The protein must exhibit the following characteristics: Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate; The sulfur is donated as persulfide by IscS.; Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor; This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway.6. The protein design should take into account the physiological conditions of the thiamine diphosphate biosynthesis pathway. The designed protein sequence is ",
    "response#1": "VIKLFPEIIVKSRPVRKQFISQLRKNIKKILSAIIEQPQVHGTWDRLEVDLKQDDYHLESQLIDILQRTPGIEFILKVNTHQLSANVSNYMDEIAKIVVEQYKDKVENKTFCLRAKRTGTHPFKSIEMERQIGGALYQNSNSAGVKLKNPEVLVQLELKDDELFAVQERYDGLGGFPIGQQESCVSLISGGFDSTVSSYLMIKRGIRTHYLFFNLGGITHEVGVKQVAHYLWEKYASSHRVLFITIPFEPVVAEILNKVDQSQMGVILKRMMLRVADKVAEEFNAPAIVTGEAVGQVSSQTLTNLSVIDKVSNQLVMRPLIVMDKPDIVKLSRKIGTEEFAAVMPEYCGVISNKPTTRAKIEKIEREEGRFDIVKLFPEITIKSRPVRRRFIQRLQNNLQIQLKRISDNITVSGFWDKIEVVLPQESLASYEAVCSAMQQTPGVAFFIQVAEYPLGDFQDVFEKTLPLYVDRIKGKTFKVRVKRHGKHDFTSVDLERYVGGGLLQHSESAGVNVHTPDVYVDMEIRESNLYVVEKQLQGLGGYPLGSQEAVLSLISGGFDSVVASYMTMRRGSKTHFLFFNLGGKAHEIGVKQVALHLWQQYGSSARVRFISVPFDEVVGDILQNVHHSHMGVVLKRQMMRAAEQVADFMKIDAIVTGESIAQVSSQTIPNLTVIDQATKKLIIRPLITMDKQEIVDKTKMLGCFDIVKMIPEYCGVISDRPTTQAKLNKVLEEEACLNVLVRYSEVAIKRGSVRREMEALLVRSIREAAAGCGEVKFRLEPGRIFVYGDDQCVAKAASRVFGVKSVSPATEYSFADLEDLASKAASRWRDEVVGRRFAVRVHRAGSHSFTSKDVAVRVGALLATAGGSVDLERPEVEIFIEVREGRAYTYREIYEGPGGLPLGSEGKVLALVSGGIDSPVAAWYMMRRGAYVDVLYCNLGGVLTEAAALRVVEKLLEWAYGYDARVLVADCAPIADAIRRNVDRHLWSIAFKRALYRLAERAARRVKAEALVTGESLGQVSSQTLQALAAVEAGIDMPVLRPLIGLDKEEIVRMAQRIGTYDLSISVPEYCGIFSREPRRWASRGEIEL",
    "response#2": "IVKLHPEIAIKSKSVRKRFTKLLEKNIEILLSRIDEQIKVKNNWDNITVTSAKSDPSTRSALIDNLKRVPGIVQFIEVQETHFDSLDDIYQRTLTLAGHTIENKTFCVRVKRRGNHDFTSSDVERYVGGGLNQNVSGASVKLSKPEVTVKIEIKDDLAYIVTHTHYGIGGFPLPTQEDVLSLISGGFDSGVASYQMIRKGARTHFLFFNLGGAAHEIGVKQVSHYLWKQYSSTHRVKFITVDFEPVVAEILENIENGQMGVVLKRMMMRAGSQVAERFNIPALVTGESIGQVSSQTLANLNVIDRVTETLIVRPLIQHDKADIIKIAREIGTIEMAEAMPEYCGVISKKPTVKAKLETILEEEQKFDFDVIGTQENVLSLISGGFDSGVSSYMMMRRGCKVNYCFFNLGGNIPEIGVRQTAYHLWYRFGLSHKVRFISINFASIVNEILTKVNCHQMGIVLKRMMIRAASSIAERYGIQALVMGESMGQVSSQTLTNMRLIDNVSNILILRPLISHDKEHIIKIAKQIGTEEYAIQMPEFCGILSKKPTVRGSQEAVLSLISGGFDSVVASYMTMRRGSKTHFLFFNLGGKAHEIGVKQVALHLWQQYGSSARVRFISVPFDEVVGDILQNVHHSHMGVVLKRQMMRAAEQVADFMKIDAIVTGESIAQVSSQTIPNLTVIDQATKKLIIRPLITMDKQEIVDKTKMLGCFDIVKMIPEYCGVISDRPTTQAKLNKVLEEEACRHPFPGPGLAIRCPGEVTSE",
    "response#3": "IVKLHPEIAIKSKSVRKRFTKLLEKNIEILLSRIDEQIKVKNNWDNITVTSAKSDPSTRSALIDNLKRVPGIVQFIEVQETHFDSLDDIYQRTLTLAGHTIENKTFCVRVKRRGNHDFTSSDVERYVGGGLNQNVSGASVKLSKPEVTVKIEIKDDLAYIVTHTHYGIGGFPLPTQEDVLSLISGGFDSGVASYQMIRKGARTHFLFFNLGGAAHEIGVKQVSHYLWKQYSSTHRVKFITVDFEPVVAEILENIENGQMGVVLKRMMMRAGSQVAERFNIPALVTGESIGQVSSQTLANLNVIDRVTETLIVRPLIQHDKADIIKIAREIGTIEMAEAMPEYCGVISKKPTVKAKLETILEEEQKFDFDVGKVRDVLVCIPGISHIEEVIETDFTDFEGALQFVIDNMGPALTHKSFAVRVKRRGKHEFSSQDLAVYIGGGIRARVEGATVKLVQPQETVQLWLENDTLSLLVRDPDVVTTAIKEIDIREIAQRTEEEVVEVETVTGFGPNDVVLDIRSPDDQESKPLVVEGVDVVTLPFYKLSTKFGDLDQSKTWLLWCERGVMSRLQALYLHEQGFTNVKVYRP"
  },
  {
    "reference": "MQRITITIDDDLLETIDKISEQRGYASRSETLRDLVRDAVTREQAAVDSETKCYATLTYVYEHETRDLSRRLTTTQHHHHDLSVSTLHVHVDGHDCLEVSVLKGTVGEIKSFADSVVTQRGVRFGNLHLIPSEHGPHDHGPHSHD",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Transcriptional regulator.2. The designed protein should be optimized for DNA binding, DNA-binding transcription factor activity, nickel cation binding activity in order to improve response to nickel cation. The designed protein sequence is ",
    "response#1": "MGVISLIYDHHQSQLQKKVTDLQHDFHHHIVSTTHVHMDHDNCLEVIIVKGRAGDVIELADGLAALKGVRDSNLAS",
    "response#2": "GVLTIIYDHHQRDLTRKMIDIQHSKYVNILCSTHVHLDHNNCLETIIIKGKPSEIEKLSIEIGGLKGVKFSKLL",
    "response#3": "MGVVTVVFDYETTTVEEKMMHLRHEHEGLVASNFHSHVGGHHCMELFVLEGSLESISTFVGKIRATKDTLTIDYSD"
  },
  {
    "reference": "MNHFSGHDEIKVFCGNAHPSLAKDVCAYLGIPLGKMEVIEFKNENIFVRIMENVRERDVFIIQPICSPVQRNLVELLITLDAFKRASAERVTAVLPYYGYSRTDKKDQPRVPITARLVADLLTTAGANRILTVDLHAPQIQGFFNIPVDELTALFTLSDYWRNKNLKDIVVVATDIGISKRARDLAEKLDAPLAIIEKRRLGNMDTTETLNIIGDVKGKRAITVDDEIDTAGSLVNAVDALLKAGAKEVYSCCTHPVLSGPAIERISKSPVKEVVVADTIPVNGAKKLDKIVVLPMASLLGEAIHRIHTGMSIGSMFED",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein's active site must be carefully engineered to ensure that the amino acids involved in ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-   diphosphate + AMP + H(+) are properly positioned for optimal catalysis.2. The designed protein should have magnesium ion binding, kinase activity, ATP binding within cytoplasm to regulate nucleotide biosynthetic process, phosphorylation, 5-phosphoribose 1-diphosphate biosynthetic process, ribonucleoside monophosphate biosynthetic process.3. The protein design should consider the endogenous proteins and enzymes present in the Metabolic intermediate biosynthesis pathway to avoid interference with native metabolic processes.4. The designed protein should have a high specificity for ATP, D-ribose 5-phosphate binding, with minimal off-target effects.5. The protein contains novel Ribose-phosphate pyrophosphokinase N-terminal domains that confer a unique function or activity.6. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "MKIFAGNATPKLANLIAKRLYTQLGEATVSRFSDGEISVQINENVRGDDIFIIQSTCAPTNDNLIELVVMIDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKIVADFLSSVGANRVVTVDLHADQVHGFFNIPVDQLIGSPILLEDMFELNLENPIVVSPDIGGVIRARAIAKLLNDADMAIIDKRRPRANISQVMHVIGDVLGRECVLVDDIIDTGSTLCKAAEALKEHGAKRVFAYATHPVFSGNAMDNLRKSMIDEVVACDTIPQAAPAKYPKITQLTIAPLLAEAIHRVHEGGSVSALFND",
    "response#2": "MKIFAGRAGRNLANLMCDHLNIPLAEGHTELFPDGELIVKVNEDVRGRDCFVVQSTCDPVNANLMELLIYIDCLRRASAKRITAVIPYFGYARQDRKDEGRTPITAKLVANLIATAEAAPVINEYLRNKGYSDDNIVVVSPDEGSIKRAIGHVKRLGGNLAIVDKRRASAMETTQQNLIGGPIEGKVALMFDDMISTAGSICGAAELVAAAGASEIHVAATHGVLCGPAIEAAPVLIDYFAQKNIPDLVVVAPDVGGVKMARAYAKRLGADLALTDKRRPRPDSVEIMNIIGDVEGKNAVLFDDLVTTGGTLCQAAETIREQGARDIYAGVTHGVFSPGALARIEQSPIKELVITDSFQSASRLAAPVLNEHFSELGMEGDDIVVVSPDEGSIKRALGHSKRLGGTLAIVDKRRTSALEVRQSTIIGGPVEGKVALLFDDMISTAGSICGAARLVHEAGAREIHIATTHGVMCGPAIEKLREAPIDSIVVTDTLPCDAGAQLSNVRVLSVAPLLGEAIRRIHRGESLSAMFV",
    "response#3": "MKIFAGRAGRNLANLMCDHLNIPLAEGHTELFPDGELIVKVNEDVRGRDCFVVQSTCDPVNANLMELLIYIDCLRRASAKRITAVIPYFGYARQDRKDEGRTPITAKLVANLIATAEAAPVINEYLRNKGYSDDNIVVVSPDEGSIKRAIGHVKRLGGNLAIVDKRRASAMETTQQNLIGGPIEGKVALMFDDMISTAGSICGAAELVAAAGASEIHVAATHGVLCGPAIEVPVLLEYLSTKQLSDIVVVSPDVGGVARARAFAKKLGDSPLAIIDKRRQAHNVAEVLNVVGDVKNKTAVLVDDMIDTAGTISEAARLLRKEGARQVYACATHAVFSPPAIERLSTGLFEEIIVTNTLPIGGEAQLKGLRVLPIAPLLARAIREVFYDGSVTSMFKGD"
  },
  {
    "reference": "MNGLMIAAPSSGSGKTTVTLGLMRALRRRGLSIAPGKAGPDYIDPAFHTAASGKPCFNYDPWAMRPELLLANAAAAAEDGSVLIMEAMMGLFDGAADGTGAPADLAAALGLAVILVVDCARLSHSVAALVGGYARHRDDVRVAGVILNRVGSDRHEGMLRDALAGIAMPVFGVLRQDAALKLPERHLGLVQAGEHGSLEAFIDHAAMRVASGCDLEAVLAAATPLTVGERAGTLKPLGQRTAVARDVAFAFCYEHLLSGWRGQGAEVTFFSPLADEAPDPRADAVYLPGGYPELHAEQLSNASNFRAAMHKAAGGGARVFGECGGYMVLGEGLVAADGGRYEMLGLLPLVTSFAERKRHLGYRRVTPVDDVFFRGPMTAHEFHYATIVSEGAAEPLFTVRDAAGLDLGRAGLRRRNVAGSFMHLIDFSE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile to enable effective catalysis of 2 ATP + 2 H2O + hydrogenobyrinate + 2 L-glutamine = 2 ADP + 2   H(+) + hydrogenobyrinate a,c-diamide + 2 L-glutamate + 2 phosphate.2. A protein that can modulate the overall flux of metabolites through the adenosylcobalamin biosynthesis, Cofactor biosynthesis, cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 9/10 pathway, enhancing or suppressing its activity as needed.3. The protein design must be able to perform cobyrinic acid a,c-diamide synthase activity, ATP binding, hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) activity in order to contribute to cobalamin biosynthetic process.4. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.5. For general function, the protein need meet that Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of hydrogenobyrinate, using either L- glutamine or ammonia as the nitrogen source. The designed protein sequence is ",
    "response#1": "MIAAPASGSGKTTVTLGLLRALKRRGEALAPVKAGPDYIDPAYHKAASGVDCFNLDPWAMRPELISALSSRMTESGARLLVAEGMMGLFDGAMDGKGSSADLARLLDLPVVLVVDCARQSHSIAALVWGFSQFRKDVLIAGIILNRVGSSRHEAMLRGALEPLRIPVLGALPRDTALSLPERHPDLAXXSAXDPXAXGRIAVARDAAFCFVYQDNLRLLREAGGELLFFSPLADGALPEGISGIYLPGGYPELYAERLAVNVPMLDAIRGAARDGMPLYAECGGFIYLTRGMEDSQGAPLADFAGIFPVRARMLPKRKALGYRQVECLSVSILGPAGETARGHEFHYSEICEMPVDVTRTYSVTRQGAFLGQEGYCLSNCLASYVHLHFGSEPLLASAE",
    "response#2": "MLAGVSSGIGKTTISMALMSVFNNVSPFKVGPDYIDPGFHEFITGNKSYNLDIFMMGEQGVKYSFYKHHKDISIIEGVMGLYDGMDNSLDNNSSAHIARFLGVPVILVLDGVGKSTSIAAQVLGYKMLDPRVNISGVIINKVSSAKTYAIFKEAIEKYTGVKCLGFVEKNDKLNIEILSLLINDKGYGLIRMENGELVAIREEKDCSEEELAINETNTGINMVEAEWLWEHIKNINPNNAQGEYYLTDIVALAVAENKSVVSLHPNDEMEVEGVNNRLHLAQLERELQKKLVAHFMDE",
    "response#3": "MIAAPASGSGKTTVTLGLLRALKRRGEALAPVKAGPDYIDPAYHKAASGVDCFNLDPWAMRPELISALSSRMTESGARLLVAEGMMGLFDGAMDGKGSSADLARLLDLPVVLVVDCARQSHSIAALVWGFSQFRKDVLIAGIILNRVGSSRHEAMLRGALEPLRIPVLGALPRDTALSLPERHGWRADGRLRIAVARDAAFCFVYQDNLRLLREAGGELLFFSPLADGALPEGISGIYLPGGYPELYAERLAVNVPMLDAIRGAARDGMPLYAECGGFIYLTRGMEDSQGAPLADFAGIFPVRARMLPKRKALGYRQVECLSVSILGPAGETARGHEFHYSEICEMPVDVTRTYSVTRQGAFLGQEGYCLSNCLASYVHLHFGSG"
  },
  {
    "reference": "MIPLLDLTRTDLRDLMQQWGEPTYRGNQIYDWLHKNLVTDPAQMTNLSKKLRARLAAETSVNPLELITQVDSEDRLTRKALFRLPDGATIEVVLMLYNYRRTVCISTQVGCGMGCPFCATGQGGLVRNLTAGEIIAQVHWFERWLREEDGRENSLKVERPSRLSNIVVMGMGEPLANYDALLQALRAIADPDTFSLSARAITVSTVGLIPMIDKLVEEKLPVRLAVSLHAATNRLRNRLVPINKRYPLEALMAACRRYQQKTNRRISFEYALMRGVNDAPQHAEQVADLLADLRCHVNLIPLNPTAGSRYQPSNEQTAQRFQEILQEAGITTTMRLRRGVEINAGCGQLRQASQQSDPK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The presence of [4Fe-4S] cluster is necessary for the protein to function in the desired environment.2. The designed protein should be optimized for metal ion binding, rRNA (adenine-C2-)-methyltransferase activity, rRNA binding activity in order to improve rRNA base methylation.3. The designed protein must have at least one Radical SAM core domain.4. The designed protein should be able to compete with endogenous proteins or inhibitors for [4Fe-4S] cluster binding.5. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.. The designed protein sequence is ",
    "response#1": "DKERGTLCVSSQVGCKMNCLFCQTGKQGFEGNLTAGDIINQIYSLPERERLTNVVFMGQGEPMDNLDNVLRATRILTSAYGYAWSPKRITVSSVGVKNKLRRFLDESDCHVAISMHSPLHELRLSLMPAERQMPIADVVELLRQYDFTGQRRVSFEYIVFDGVNDTQAHGREIVRLLDGLECRMNLIRFHAIPDVALRTTDDRRMEALRDYLTSHGVFTTIRASRGQAEVDDAVAE",
    "response#2": "DKERGTLCVSSQVGCKMNCLFCQTGKQGFEGNLTAGDIINQIYSLPERERLTNVVFMGQGEPMDNLDNVLRATRILTSAYGYAWSPKRITVSSVGVKNKLRRFLDESDCHVAISMHSPLHELRLSLMPAERQMPIADVVELLRQYDFTGQRRVSFEYIVFDGVNDTQAHGREIVRLLDGLECRMNLIRFHAIPDVALRTTDDRRMEALRDYLTSHGVFTTIRASRGQGKIGG",
    "response#3": "SSQAGCGMNCPFCATGQAGLTRNLSTGEIVEQVAGGQRALNRGDIAGGPGRVSNIVFMGMGEPLANYKAVLGAVRRITEPSPSGLGISQRGVTVSTVGLVPAIHKLTEEGLSVRLAVSLHAPDDELRDTLVPINTRFKVREVLDAAFGYARKSGRRVSIEYALIKDVNDHAWRDPTDE"
  },
  {
    "reference": "MTLKELLKEVNTIQIIGNDQVEVTNLTQDSRKISDNTLFFAIKGAKTDGHSFISQVVVEKVKVVVCEVLPTTLPQEVTFVKVADTTIAMGKIAANFYGNPSKKLKLVGVTGTNGKTTIATLLYQLFGLLGHKVGLISTIAIYIDQQRMETKNTTPDVLTLNEVMHRMVEQGVTHCFMEVSSHGVALHRIEGLHFSGGVFTNLTQDHLDFHNTLADYRDTKKKFFDSLSKTAFALTNIDDKNGSYMLQNTPAKRYSYALKTMADFRLSVLENTFSGMLLRIETQELWVSLIGKFNAYNLLAIYATAKLLGVDTLQALLALSRLTPVAGRFQYFVAPDKRTLIVDYAHTPDALENVLETIRSIKQAGQQLITLVGCGGNRDKGKRPIMAHIATSLSDKVILTSDNPREEDPEAILQEMEAGIRGENQHKYMTITDRRQAIKVACHLAHPGDIILLAGKGHETYQEIKGVKTHFDDLEEGKKVMSEK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein with ATP binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity functions is required to enable regulation of cell shape, cell wall organization, peptidoglycan biosynthetic process.2. The protein design must feature the Meso-diaminopimelate recognition motif motif.3. The protein should be able to bind UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate ligand in a variety of conditions4. The protein should be designed to have high stability and activity towards ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.5. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.. The designed protein sequence is ",
    "response#1": "MKLGQLLAQAGLPTAELAGLCADSRRVAPGDVFVALAGASSHGLRYAAEVRAAGAVAVLYDPAEALPADPAAVAGALAVPGLRSRLGELADAYDGDPSSRLALLAAEDTRRAFALLAAGFFRPRPDRIIGVTGTNGKTSTCQIIAKLLQATQGMKTAVMGVTGTNGKTSTCQIIAKLLQATQQSCSVLGTLGAGNVDALTPLANTTPGATELHHYLNEFKAKGIENLAMEVSSHALSQRRLTNDIIDIAVFTNLSRDHLDYHQTMNAYSAAKKTIFSSTGEQIAVLNIDDSQAQQWLTHWPQSQPYLVYARCEVSKAKQHSCYLVAENISHNQQGVSFDLVTHLGSKKINSPLLGDFNIDNLLAAIAVALRDGVPLETALAALEPLAGAPGRMTRLGGGSAPLVLVDYAHTPDALALALASLRGHVRGRLWCVFGCGGERDRGKRAAMAAVAEAGADRVIVTDDNPRGEDGDAIVADILAGFSEPSRHRVLRDRRAAIHAAIGAAVTGDGVLLAGKGHETTQTIGTVTIPFDDRQVAREALAGRA",
    "response#2": "MRLRDLLAPVTGLPVQVPPGALYCCIPGDRVDGHDFAGRAVELGAVALVCEHSLPGLPGVPQVPVPDGRAAWGLLAGHLQGSPEQGLALVGVTGTNGKTTTTLLIEYLLTYVGARTGLIGVTGTNGKTSVATFLRMIWQELGLPAANLGTTGVEGDFEAPLKHTTPEPITLHRTLRDATQAGVECVAMEASSHGLEQRRLDGVQLTAAGFTNFTQDHLDYHATFDDYFDAKAGLFARVLPEDGTAVINIDDMRGTDMAAIAKGRGQHVITVGRHGADLELLAQRFDATGQDIRFSFGGEIYQTRLNLIGGFQAENVLLAAGLALASGLPLEHILDALPRLRGVPGRMERIAAPGQPLAVVDYAHTPDALDKALAGLRPLAAQRGGALWCVFGCGGDRDPVKRPMMAAVVEKQADRVVVTSDNPRSEKPAAIIGQILLGLARPEAAQVEADRARAIADTLAQAAPEDVVLLAGRGHEDYQILGTEKLHFDDREQARAALAARVGGGVA",
    "response#3": "MKLKTILQQASIVVEGDAGLPVITGVCYDSRKVEPGNLFVCVVGEKTDGHLFAQQAVQSGAVAVMGERIEAAVGQVCDNVPNRQMLVLADSNRRAGALAERLYGNPSRQLKLVGVTGTNGKTTTTYLVRSIFERGGWPTGIVGTLDGPRTTPESPDLQRSLAAFRDGGRVAAALEVTSHALVQGRVDGICFDVAVFTNLSQDHLDYHATMDAYFAAKSLLFEPERSRVAVVNLGDPYGRRLAERRRSDVIGYSLADAHDLELGPTWSRFELAGEPVEIRLGGVFNVENALAAAAAALVSGIELETVVTALASLLPVPGRLEEVPAPGFRVFVDFAHTASALARMLDSLRGAARETGGRLIVVFGCGGDRDRTKRPEMGRAVCARADAVYLTSDNPRTEEPRAIIDDVLAGTAGGDADLHVEADRERAIRSALADAREGDVVVIAGKGHESYQIIGDTKLPFDDRAVARAALAAREGRAA"
  },
  {
    "reference": "MEMDTIAAISTALGEGAIGIVRLSGDQAIAIGDKLFKGTKRLEDTPSHTIVYGHLMDDASEEAIEEAMVTVMRAPRTYTREDIVEINCHGGLVSVNRVLQAVLAKGARLAEPGEFTKRAFLNGRIDLSQAEGVIDLIRSKTDRAMNVALRQVEGRLSKKIGKLRQALLETIASIEVNIDYPEYDAETMTAKLINEKMTIVLAEIEALLATAKQGKVLREGLATAIIGRPNVGKSSLMNSLVHEAKAIVTDIPGTTRDTLEEYVNVRGVPLRLIDTAGIRETEDIVERIGVERSRQALKEADLILLVLNYAEKLSKEDEALFEAVKGLDVVVIVNKIDQTGRIDMERVRQLAGENPVVATSFLQEEGVDQLEEAISRLFFAGGVEGADLTYVSNARHIGLLNQAKAHAEEAIAASLTDVPVDLIQIDVTRTWELLGEIIGEDVQDSLIDQLFSQFCLGK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34.2. The protein must exhibit metal ion binding. The designed protein sequence is ",
    "response#1": "SPTTEANLWALMIILLKLPNDKIPHFMRMWKNSNAMERQVADIVAFFDLISSHAPNNYDLYKAGLETIVSTIDLAHILGQPVNGSALVDSYEALPIKNNRDLVIDGHFLLKNGIPAGPRVGLLLEEIKKAVLEGVISNNEAAITEFL",
    "response#2": "SPTTEANLWALMIILLKLPNDKIPHFMRMWKNSNAMERQVADIVAFFDLISSHAPNNYDLYKAGLETIVSTIDLAHILGQPVNGSALVDSYEALPIKNNRDLVIDGHFLLKNGIPAGPRVGLLLEEIKKAVLEGVISNNEAAITEFC",
    "response#3": "SPTTEANLWALMIILLKLPNDKIPHFMRMWKNSNAMERQVADIVAFFDLISSHAPNNYDLYKAGLETIVSTIDLAHILGQPVNGSALVDSYEALPIKNNRDLVIDGHFLLKNGIPAGPRVGLLLEEIKKAVLEGVISNNEAAITEFC"
  },
  {
    "reference": "MAMLSFEKKYRVRGGTLVGGDLFDFWVGPFYVGFFGVTTLFFSVLGTALIIWGASQGPTWNLWQISIAPPDLKYGLGVAPLMEGGLWQIITVCAIGAFVSWALREVEICRKLGMQYHVPIAFSFAILAYVTLVVIRPILMGAWGHGFPYGIFSHLDWVSNVGYQYLHFHYNPAHMLAITFFFTTTLAMSMHGGLILSAANPKKGEPMKTTDHEDTFFRDAVGYSIGSLGIHRLGLFLALSAAFWSAVCIVISGPFWTRGWPEWWGWWLNLPIWSQWPLK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis..2. The designed protein should have a high specificity for Fe cation, a ubiquinone, (7R,8Z)-bacteriochlorophyll b binding, with minimal off-target effects.3. A protein that can perform metal ion binding, electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity, bacteriochlorophyll binding in plasma membrane, plasma membrane light-harvesting complex to support photosynthetic electron transport in photosystem II, response to herbicide.4. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "MGARLAALGIL",
    "response#2": "MTYC",
    "response#3": "MSWGKR"
  },
  {
    "reference": "MAYNWWVIRVLCNKSLEEVIFWRLDSFGCRGTASQIQGKNCLVLAYLPEEQTQLLDLSALSLNFRQDALCMNFPVPIMDWYLIEEEDWASSWKQYWQPQEIGDHFLIHPAWLDLPEQSDKIILRLDPGAAFGTGTHPTTQLCLESLEMRLGLEPHPEAIIADIGCGSGILSIGAALLGVHQIYAVDIDPLAIRSTINNGKLNQIKAEKLIVEQGDIKKLIKLCPKPVDGIVCNILAEVIIDIIPHLTAIAKPKTWGIISGILLEQAKLIGDTLEQNGWVVATLWKKEEWCCFNIRYS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Methylates ribosomal protein L11.2. The protein must exhibit histone lysine N-methyltransferase activity. The designed protein sequence is ",
    "response#1": "PKLYIASSGVAGDGIFSTFDIDELEVLAKIPRRIILSPRNSRFGDSLYTHFNESNRSDDINFDNRDQVGLVMLVITVILENITDSPWNAYLNTLDETCMPDSPLLWKDKTCLEGTSMLDVINTNLRVYKNQYDQLVRPYFYKHADLKQLCPKWNQYLETCVLVQSRCFYVNSYYGLSLIPFFDIFNHKSGPAIASLHCQESNDHKGDIKIEFISFQYIRKMSEIFNSFGNFAADC",
    "response#2": "PKLYIASSGVAGDGIFSTFDIDELEVLAKIPRRIILSPRNSRFGDSLYTHFNESNRSDDINFDNRDQVGLVMLVITVILENITDSPWNAYLNTLDETCMPDSPLLWKDKTCLEGTSMLDVINTNLRVYKNQYDQLVRPYFYKHADLKQLCPKWNQYLETCVLVQSRCFYVNSYYGLSLIPFFDIFNHKSGPAIASLHCQESNDHKGDIKIEFISFQYIRKMSEIFNSFGNFAADC",
    "response#3": "PKLYIASSGVAGDGIFSTFDIDELEVLAKIPRRIILSPRNSRFGDSLYTHFNESNRSDDINFDNRDQVGLVMLVITVILENITDSPWNAYLNTLDETCMPDSPLLWKDKTCLEGTSMLDVINTNLRVYKNQYDQLVRPYFYKHADLKQLCPKWNQYLETCVLVQSRCFYVNSYYGLSLIPFFDIFNHKSGPAIASLHCQESNDHKGDIKIEFISFQYIRKMSEIFNSFI"
  },
  {
    "reference": "MVFDAVGNPQTILLLGGTSEIGLAICERYLRNASARIVLAVMPGDPGRDAAVEQMRKAGASAVDVVDFDALDTESHPAVIDQAFAGGDVDVAIVAFGLLGDAEELWQNQRKAVQIAGVNYTAAVSVGVLLGEKMRAQGSGQIIAMSSAAGERVRRSNFVYGSTKAGLDGFYLGLGEALREFGVRVLVIRPGQVRTRMSAHVKEAPLTVDKEYVAELAVTASAKGKELVWAPGAFRYVMMVLRHIPRPIFRKLPI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be designed to localize specifically to periplasmic space in order to perform its role in capsule polysaccharide biosynthetic process more effectively.2. The protein have enhanced NAD(+) + trans,octa-cis-decaprenylphospho-beta-D-   arabinofuranose = H(+) + NADH + trans,octa-cis-decaprenylphospho-   beta-D-erythro-pentofuranosid-2-ulose. The designed protein sequence is ",
    "response#1": "IPDVLRPDINVAYTDTDGTGQRADRRPPGAERYYREIDAKILEVTGVPRNQTVVLTADYSFLSFYPYYGFQGLTSHYANPLAQFDKRAAAIEGWATLGSADDFVAALDELPWEPPTVFLMRHGANDTYTLRLASDVYPNQPNVRRYHVELDSAVFDDPRFEVSDHGPFVLAIR",
    "response#2": "IPDVLRPDINVAYTDTDGTGQRADRRPPGAERYYREIDAKILEVTGVPRNQTVVLTADYSFLSFYPYYGFQGLTSHYANPLAQFDKRAAAIEGWATLGSADDFVAALDELPWEPPTVFLMRHGANDTYTLRLASDVYPNQPNVRRYHVELDSAVFDDPRFEVSDHGPFVLAIR",
    "response#3": "IPDVLRPDINVAYTDTDGTGQRADRRPPGAERYYREIDAKILEVTGVPRNQTVVLTADYSFLSFYPYYGFQGLTSHYANPLAQFDKRAAAIEGWATLGSADDFVAALDELPWEPPTVFLMRHGANDTYTLRLASDVYPNQPNVRRYHVELDSAVFDDPRFEVSDHGPFVLAIK"
  },
  {
    "reference": "MSRNLAQQDQPLPSIPPARSIVLVGMPGAGKTSIGKRLAARLRLPFLDADTEIEAAAGIPITDIFSRYGEPHFRDGERRVIGRLIAQGPLVLATGGGAFADPGTRAAIKAAPVTTIWLRCSMPVLLRRVAGREHRPLFLHQDPQAVLARLAAARHPLFAEADIVIDCNDEHPEVTTRRVGQAGLNHRPPTRLPVPLGRRGYDVLIGPGLLGRAGALLADALPARRVAIVSDEAVAALHLATLRVGLEEAGFAIAAEILVPPGEAAKSMAQYQRVVEAILGARPDRRLAVLALGGGVVGGLAGFAAATILRGLPFVQCPTTLLAQVDSSVGGKTGINTRQGKNLLGAFHQPRAVLADTATLATLPLRELRAGWAEVAKHGLLQGPLWEWCEANGPAAMAGDPAALTHAVLESARLKSSVVVADEFEEAVDGGRALLNLGHTFGHALEAECGYDGTLLHGEAVALGLGLAAMLSARLGHCSQEWPGRVISHLQAIGLPARLRDLPRGFSAATLVGRMRADKKARDGALRFVLFAEAGRAFTADDVDAALVETLLRDEGCTA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be involved in phosphorylation by localizing to cytoplasm and exhibiting magnesium ion binding. The designed protein sequence is ",
    "response#1": "IPDDTHEFAINASNARFWEEPVQRYVGELQAGKTGPRGKDFNMRWVASMVADVHRILTRGGIFMYPLDEKCRAQGGKLRLMYEANPMAMLVEQAGGAATTGRERILDLMPTKLHQRVPVILGSRNEVERVTAYR",
    "response#2": "IPDDTHEFAINASNARFWEEPVQRYVGELQAGKTGPRGKDFNMRWVASMVADVHRILTRGGIFMYPLDEKCRAQGGKLRLMYEANPMAMLVEQAGGAATTGRERILDLMPTKLHQRVPVILGSRNEVERVTAYL",
    "response#3": "GTLTYIQSRCQEALSNLHTDPETGSHRLVALLPATLQDCEEIHNEVEFEWLRQYWFQGQRYARFCSWWTRPMEQLEKDWKLMETMTMLFLAAVEEEGQAAEGRREMAEILLSALTDRHQQRQTWRDRCHSSLAQTLPPEEAPVDRPFWGVDDPSMPLPFDLADIINRVL"
  },
  {
    "reference": "MNVDFGNALAAEAVTGVSEASLERLDDRVADAHDRIEANIEARRFGYASLALPTDTDPDAIYEAVSGFDPEAVLTVGIGGSALGAETITAALGAESHYTLDNVDPAPTRQLLDELPLSSTLVNVVSRSGTTAETLANFLVVREAMADAGVDWTDRTVVTTGAEGPLRTLADAHDLPSCTVPEGVPGRFSALSAVGLLPAAALGCDIEAVLAGGAAGRESLAPSLFESPAYAYGAVAYATEQRGATTNAIVPYAEQLEPFAEWFAQLWAESLGKDGLGQTPARALGATDQHSQLQLYRAGRKDKLVTLVRPRERAGVDIPETDIDALSYLGGESLESLLDAEFEATEASLAAAGQPNVRIELDSLDAHGVGELLYGMEAACILYGELLGIETFTQPAVEWGKRAARGLLGGGDFEEAEAVADKTVRRVE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein should be able to manipulate the activity of enzymes in the D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 2/4 pathway, either by activating or inhibiting their function.2. The designed protein must be able to perform glucose-6-phosphate isomerase activity under specific conditions. The designed protein sequence is ",
    "response#1": "ESHAILPYDHYLRSLPAYLEQADMESNGKSVDRDGRVVDYPTGQIIWGATGINGQHAFYQLLHQGTKMIPADFIVSITPHTELQEHHDILIANFLAQTEALMRGRTREETLEEMGRSPGDPSVQHRVFEGNHPSNAILLKKLTPHTLGMLIALYEHKIFVEGVIWNLNSYDQWGVELGKQLAGRKGLA",
    "response#2": "VPYSHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGTRLIPCDFIVPMTAQGREDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERERLAPHKEFPGNRPSNSILIDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGKQLAKTLGLV",
    "response#3": "VAPYHQGLRRLPAYLQQLEMESNGKCVDRDGEPLPFATSPVVWGEPGTNAQHAYFQMLHQGSDVIPVEFIAVRDTSHAHLDLPPALQPLLAEQQRKLLANCLAQSQALMAGKSEAQALAERAPTAAADMPRDVLARHRSFPGNRPSTTLLLERLDPATLGALIAMYEHRVFTSGAVWGINSFDQWGVELGKAMCSEGRIAAMNILQNK"
  },
  {
    "reference": "MNVGIKGFGAYAPKNIIDNAYFEQFLETSDEWISKMTGIKERHWADEDQDTSDLAYNASIKAIEDAGIQPVDIDMIIVATATGDMPFPSVANILQERLGTGKVATMDQLAACSGFMYSMITAKQYIQSGDYKHILVVGADKLSKITDMTDRSTAVLFGDGAGAVVMGEVAEGRGIISYEMGSDGSGGKYLYLDRETGKLKMNGREVFKFAVRIMGDASTRVVEKAGLSSEDIDLFVPHQANIRIMESARERLGIEREKMSVSVNKYGNTSAASIPLSINQELQNGKIKDDDTLVLVGFGGGLTWGAIVIKWGK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids..2. A protein with beta-ketoacyl-acyl-carrier-protein synthase III activity functions is required to enable fatty acid biosynthetic process.3. The designed protein must have a active site active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "YAQMIHGKTNCDGYKEQGISYPASIIQKLLLEEFYQECAIAPSVLEYLEAHGTGTKVGDPQELQAIDEIFCTGRSHPLLIGSVKSNLGHSEPASGLCSIAKMCIAYNTGFIPPNIHFF",
    "response#2": "VVIAGIAGKLPESENLQEFWENLLNGVDMVTEDDRRWKPGIYGLPKRNGKLKDISKFDASFFGVHPKQANTMDPQLRLLLEVSYEAILDGGINPGTLRGTDTGVWVGASGSEAAEALSQDPEELLGYSMTGCQRAMFANRISYFYDLTGPSVTIDTACSSSLIALENAYKAIRHGQCSAALVGGVNLLLKPNTSVQFMKLGMLSPDGTCKAFDVSGNGYCRSEAAVVVLLTKKSMAKRIYATIVNAGSNTDGFKEQGVTFPSGEMQQQLVSSLYRECGIKPAEVEYVEAHGTGTKVGDPQEVSGIVNVFCQCEREPLLIGSTKSNMGHPEPASGLAALAKVILSLEHGLWAPNLHFNTPNPDIPALQDGSLQVVCKPTPVKGGLVSINSFGFGGSNAHVILRPNEYC",
    "response#3": "QDAPVTATDVIRALVAQKLKKELTEIPPSKAIKDLVGGKSTLQNEILGDLGKEFGSTPEKPEDTPLDELGASMQATFDGQLGKQSSSLIARFVSSKMPGGFNITAVRKYLETRWGLGPGRQDGVLLFAITAEPPSRLGSEADAKAYFDNLANKYASYAGITS"
  },
  {
    "reference": "MSTPRLVLVGLPGAGKSTIGRRLANALHCEVVDSDLLIEQDRGLPCGEVFRQLGEPAFRELEEEHVARALGTGGIVSLGGGAVISPATRALLADLPVVHLRVSAQEGARRTTGDANRPVVAAEDPVARYRQLQTERASFYDEVSDATVSSEGRDPRQTVAEVLHILESLEEERAARQTTAQSTSAQSTTTVKESPVTDLRRVHVATDHPYDVVIGRDLTGQVADAVPGAAKAVILHQPPLAELADRVAAGLRAAGIDPVLYETPDAEDAKTVSGAAACWDVCAAAGLSRQDIIVGVGGGAATDLAGFIAATWMRGIRVVQYPTTLLAMVDAAVGGKTGINTAAGKNLVGSFHEPAAVLVDLDVLDTLPIKEITAGSAEIIKAGFIRDPRILDIYEADPAAALDPRGALPELIELSVKVKADVVGQDLRESSLREILNYGHTYGHAIEQHENYRWRHGWAVAVGMVYEAELAHAAGLLGADAVARHRRILTSVGLPTSYDGAPLDVLLEVMGRDKKNRDGHLRIVVLSDREGLPYAPVRLEGPEAADLQAAYDATLTEHADQEAK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. We require a protein that can specifically bind to the metabolites produced in the chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7 pathway. The designed protein sequence is ",
    "response#1": "RHVTGHLRLPGDKSISNRSLLLGALAEGVTEVTGLLDSDDARAMLNALRDLGVVIEGPHQGRCTVHGVGLHGLKAPPGPLFLGNAGTAMRPLSAALALQPFDTTLTGDPRMSERPINRLVDALREMGAVIEYLAQEGYPPLTIRGGGSVSSQFLTALLMTAPMASAQIKSGLLLSKPYIDITLNVMPFGVPTRDHTERIFAVSAIRYPSPAVLRLEGDATSASYFLAAAGIKGVPVTGIGRHSMQGDSWFPRALRRMGARSCGSSMIVCPRGELRAAVRSDSNSIPDAAMTLATXXALARGGRRPRTIFAWRVKETDRLYAMSTELGAAGARVVPGAGLRGCPLRGRSYYVARCRRDSRMAMCFSLPV",
    "response#2": "RHVTGHLRLPGDKSISNRSLLLGALAEGVTEVTGLLDSDDARAMLNALRDLGVVIEGPHQGRCTVHGVGLHGLKAPPGPLFLGNAGTAMRPLSAALALQPFDTTLTGDPRMSERPINRLVDALREMGAVIEYLAQEGYPPLTIRGGGSVSSQFLTALLMTAPMASAQIKSGLLLSKPYIDITLNVMPFGVPTRDHTERIFAVSAIRYPSPAVLRLEGDATSASYFLAAAGIKGVPVTGIGRHSMQGDSWFPRALRRMGARSCGSSMIVCPRGELRAAVRSDSNSIPDAAMTLATXXALARGGRRPRTIFAWRVKETDRLYAMSTELGAAGARVVPGAGLRGCPLRGRSYYVARCRRDSRMAMCFSLPR",
    "response#3": "RHVTGHLRLPGDKSISNRSLLLGALAEGVTEVTGLLDSDDARAMLNALRDLGVVIEGPHQGRCTVHGVGLHGLKAPPGPLFLGNAGTAMRPLSAALALQPFDTTLTGDPRMSERPINRLVDALREMGAVIEYLAQEGYPPLTIRGGGSVSSQFLTALLMTAPMASAQIKSGLLLSKPYIDITLNVMPFGVPTRDHTERIFAVSAIRYPSPAVLRLEGDATSASYFLAAAGIKGVPVTGIGRHSMQGDSWFPRALRRMGARSCGSSMIVCPRGELRAAVRSDSNSIPDAAMTLATXXALARGGRRPRTIFAWRVKETDRLYAMSTELGAAGARVVPGAGLRGCPLRGRSYYVARCRRDSRMAMCFSLPEGQS"
  },
  {
    "reference": "VGDPIESNSIGKVIGKNRKDNCYIGSVKSNIGHLEPASGIAGLSKLALALQKGIIPPNIHFEKGNPNIAFDELKLKVPTEALKWPSKNGDTRYGGVNSFGFGGSNAHVVLSDHKSTVREPNSKEGLQVCTVSAKSTDALQSLAANFIVFLKDKKNKASFSDICAYTAIRQSHHDIRLSIVAESKKELAKSLKIYLDGESLTEISEGRVNEPKDKIVFIFSGQGPQWWGMGRQLLGTEPLFREWIEKLDGMLSRHADWSLIKELKKTEKTSRISDTNIAQPAIFAIQIALYEMWKAMGIEPGAVVGHSIGEVAAGYASGALTLDQAVLLIFHRSRVQFKATDKGRMLAVGVSNEEAKKIIKGLEDKVSIGAVNGPQMVALSGDTDVIEKISEELTEKDVFNKLLMVNVPFHSHHMEPLKAELLKSLGKFKTASTKIPFYSTVEGKIVQGKKLTPEYWFRNVREPVYFTGAIAAQIEDGFDTFIELGPHPVHAIGVEDLLTEKKVKGLVIPSLRRKEDEKRTFLSSVGMLHAWGCKIDWKLFYGDDNGWVNLPRYPWQKDSYWIESTEGKRQRLGAIQFDHPHLKRKTVSAREDSNIIWDVDLDQRTYPYIGDHKVQGPIVFPGAGHVDLVIGAARASFGDKFEFIEDVNFDSALFLPDSGEPPHIQMDISHDGGDYFIYTRPKTADAQWTMCSNGKINHVGNKFVPIPVDLEDVKKRITIPVPVKPMHDELLESGLYLGPTFRAIKKLWRSDGNWESLSEIEVHESIRP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein have enhanced dodecanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxotetradecanoyl-   [ACP] + CO2 + holo-[ACP].2. The designed protein must possess phosphopantetheine binding to support biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "IVIAGISGRLPESNNLEEFWENLIGGVDMVTEDDRRWTPGPSTAIDTACSSGLLALENAFHAIQQGHCDAALVGGVNLLLKPNTSVQFMKLGMLSPEGACKSFDASGNGYCRSEAAVAVLLTKRSAAKRVYATVVNAGNNTDGYKKEGVTFPSGEMQQRLVRSLYQESNISPEQVEYVEAHGTGTKVGDPQEVNGIAAVFCEMEREPLLMGSTKSNMGHAEPASGLAALAKVVLSLERGLWAPNLHFNSPNTNIPALSDGRIQVVDRPIPVRGGLVSINSFGFGGSNVHVILRPAEF",
    "response#2": "IVIAGISGRLPESNNLEEFWENLIGGVDMVTEDDRRWTPGPSTAIDTACSSGLLALENAFHAIQQGHCDAALVGGVNLLLKPNTSVQFMKLGMLSPEGACKSFDASGNGYCRSEAAVAVLLTKRSAAKRVYATVVNAGNNTDGYKKEGVTFPSGEMQQRLVRSLYQESNISPEQVEYVEAHGTGTKVGDPQEVNGIAAVFCEMEREPLLMGSTKSNMGHAEPASGLAALAKVVLSLERGLWAPNLHFNSPNTNIPALSDGRIQVVDRPIPVRGGLVSINSFGFGGSNVHVILRPAER",
    "response#3": "MDMDIFNISEVTGDRTKVGDPEELRALDEVFATGRTEPLHIGSVKSNLGHSEPASGLCSIAKMCVAYQTGYLPPNLHYNVPREGVDALAEGRLKVVDVKTPWNRGMSGVNSFGFGGANAHVLLKS"
  },
  {
    "reference": "MNTNNKNDFKSVYVSSRARDNILKYKYTGCDSSFISIHIMNHFWNWFVNLFPRSFAPNLITLFGFISIIVSYFVTLYYMDRMNGVAPKWLYLFNALCIFIYQTMDACDGKQARRVQASSGLGELFDHGCDAMITYLVMQTFQSSLQVGVNQISFFTTLWIMYVFFMAQLEQYSTGVMNLGHFGPTESQFSMMAGHIFTFFYGEQFWFNTIKFESFEIQYNHLVLLVVILGGVGTILLNTFSILKNGNESILTNIINLVPISILLGVSIYWGKYSTVNIFETAPHYFMGIFGILFALVTGKLILARICMDKLSPIQLIMLPLIAVILNIYKFNGELFDEILFTKVYFFVVFIVYIHFAYDVVTSLTKVLDIYCFTLKNKKQT",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. The protein should have phosphotransferase activity, for other substituted phosphate groups and be active in membrane. The designed protein sequence is ",
    "response#1": "VTVSIVGKYTEFPDAYKSLIEALNHGAISNKVKIKINWVNSREENEKPINKNLIGEKLQDSHAILVPGGFGDNGTEGKMLAVSYARTHNIPFFGICLGMQLAVIEFARNVIKLEDVHSEEFCTCKHPIVKLAGDQSVNLGGTMRLGAYKCNISPNSKMADIYSSVTILERHRHRFIINLDYKDDLEKNGLICSSMSEDGTCIEAVELENHPWFIGVQFHPEFQSKPFSPHPLFVSFIAGQ",
    "response#2": "PDAYKSLIEALNHGAISNKVKIKINWVNSREENEKPINKNLIGEKLQDSHAILVPGGFGDNGTEGKMLAVSYARTHNIPFFGICLGMQLAVIEFARNVIKLEDVHSEEFCTCKHPIVKLAGDQSVNLGGTMRLGAYKCNISPNSKMADIYSSVTILERHRHRFIINLDYKDDLEKNGLICSSMSEDGTCIEAVELENHPWFIGVQFHPEFQSKPFSPHPLFVSFIG",
    "response#3": "PDAYKSLIEALNHGAISNKVKIKINWVNSREENEKPINKNLIGEKLQDSHAILVPGGFGDNGTEGKMLAVSYARTHNIPFFGICLGMQLAVIEFARNVIKLEDVHSEEFCTCKHPIVKLAGDQSVNLGGTMRLGAYKCNISPNSKMADIYSSVTILERHRHRFIINLDYKDDLEKNGLICSSMSEDGTCIEAVELENHPWFIGVQFHPEFQSKPFSPHPLFVSFIG"
  },
  {
    "reference": "MKTWKREILGREFIVEHGKVAKQANGAVVARIGDTTVLATAVMSDSAVEGIDFVPLTVEFQERFYAAGKIPGGFIKREGKPSEMAILSARTIDRPIRPLFPKHLRNEVQVVVTVISTDSVNTPDIVGVFAASLALNISDIPFNGIVAAVRVGLVDGKIVLFPTEDQLQKSLLDIVVAGTEDTITMVEGEAKEVSESQMLQALLKAHDAIKQIIDFEKEILSYFNIEKAQIAEKTLPEEIEKEFSQLLDGNELRERLLIQGKKARSNALSEYFKKIYEILEARYGTEKIEELSVLLKDKYEEALKYKMRRIIVEEGTRLDMRKPKDIRPITCETGLLPRVHGSALFTRGETQSLGIVTLGAPMDEQIIDSLLEEGTKRFMLHYNFPPFSTGEVKPLRGPSRREIGHGHLAERALKFVLPDEDRFPYTIRIVSEILESNGSSSMATVCSGSLALMDAGVPISKHVAGVAMGLILEKDSQIVLTDILGAEDHWGDMDFKVAGTRDGITAFQMDCKVSGVSADLLERALNQAKEARLYVLEKLYETISKPRDTLSKYAPVIKVISIDPSKVAEIIGPGGKIIKALIKDYDVKISVDDLTGKVSVIGGNEEKVDAAIEQINSILKEISVGDVFSGKVTRIEPFGVFVEISPGKVGLLHQSKLITDLKTVKIGETMRVKVSNIDNLGRLQLEEFSDSPDHKHGEKRSFKRHRKNDN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'- direction. The designed protein sequence is ",
    "response#1": "LKTRVKDLAYAGIKEAVRIVAKQERHNRIGEITAQTLETLLPEYEGRESEIKGFLGDFEYELVREHIIKDGYRIDGRL",
    "response#2": "LKTRVKDLAYAGIKEAVRIVAKQERHNRIGEITAQTLETLLPEYEGRESEIKGFLGDFEYELVREHIIKDGYRIDGRQ",
    "response#3": "LKTRVKDLAYAGIKEAVRIVAKQERHNRIGEITAQTLETLLPEYEGRESEIKGFLGDFEYELVREHIIKDGYRIDGRI"
  },
  {
    "reference": "MAGTFAVVLAAGQGTRMKSNTHKILHPICGKPMIQHILDTLTETGVDRQIVVVGKLREQVMEALGDLEYAVQDEQLGTAHAVMMARPQLQGLEGTTLICAGDTPIIRGETLRAMQEHHQASGAAVTVLTAVVDNPFGLGRIVRDDAGNVLRIVEEKDATPKERQIREINSSVYLFDNALLWDALSKIDNNNAQGEYYLTDCLEVLRSDGHTVTAFITGEPKEIQGINNRAQLAVANAIIRERIAEKHMMNGVTLIDPNNTYIDVDVVIGNDTVILPGTVLEGKTVIGTGSVIGPNSHLTNAVIGDDVEVKHSVLSDCTVEAGATVGPFAYLRPKAHIGQGAKIGDFVEIKNAKIGAGTKVSHLSYIGDAEFGENINVGCGTITVNYDGFHKHKTLVGDNTFIGCNSNLVAPISIGSEAYVAAGSTVTDNVPDGALAVARERQVTKEGYTEKLKARLRDQKES",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. Mg(2+) is required for the proper folding and stability of the protein.2. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain..3. A protein that can perform UDP-N-acetylglucosamine diphosphorylase activity, magnesium ion binding in cytoplasm to support lipid A biosynthetic process, regulation of cell shape, UDP-N-acetylglucosamine biosynthetic process.4. The protein should be optimized for activity in UDP-N-acetyl-alpha-D- glucosamine biosynthesis, Nucleotide-sugar biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. The protein should have a high affinity for Mg(2+), UDP-N-acetyl-alpha-D-glucosamine.6. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "VILAAGKGTRMRSKLPKVLHPIAGKPMVQHVIDNSKALGAKSTNLVYGHGGEQLKVCLSHNDVNWVLQEEQLGTGHAVAVAKQHISDDDTVLILYGDVPLTKQSTLEKLLAVTPAKGLAVLTAAIDDATGYGRVLRDAQGDVVAIVEHKDANEAQRAIREVYSGIMAAPTALLKRWLGSLRNDNAQGEYYLTDVVKMAVADGVPVVAHRIDDEVQVAGVNSPVQLAQLERAFQLRQAERLMTAGVTVVDPATTWIDAGVTLARDVVIEPGVQLKGTTSVGEGSTVGPDSTLTNVSIGAGASVVRVHGSDSELGDGVNVGPFTYLRPGTKLGVKAKLGAFVETKAADIGAGTKVPHLTYVGDATIGEHSNIGCSSVFVNYDGVNKHRTVIGSYVRLGADNTFVAPVHIGDGAYSGAGAVIREDVPPGTLAVSAPPQRHNWKRPVKKP",
    "response#2": "LILAAGEGTRMRSSTPKVLHPIAGRPLVEHAVRAAAGLNPEHLVVVVGHGRELVGEHLAQLGTALGRPVGTAVQDQQKGTGHAVSCALATLPADVTGTVIVSYGDVPLLDTTTLAALLDEHTEAKNAVTVLTTILDDPTGYGRILRDDSGAVLRIVEQKDATAEERAIREVNTGVFAFDGRLLADALGRVRTDNSQGEEYLTDVLGILREAGHRVGAHLAADRWQTEGVNDRVQLAQLGAEHNRRTVERWMRAGVTVVDPATTWIDAGVTLARDVVIEPGVQLKGTTSVGEGSTVGPDSTLTNVSIGAGASVVRVHGSDSELGDGVNVGPFTYLRPGTKLGVKAKLGAFVETKAADIGAGTKVPHLTYVGDATIGEHSNIGCSSVFVNYDGVNKHRTVIGSYVRLGADNTFVAPVHIGDGAYSGAGAVIREDVPPGTLAVSAPPQRNIEGWVGRRRPGTPAAEAAERALAAQQDSQEGTP",
    "response#3": "GVVLAAGMGTRMKSRLPKVLHRVCGKEMVRYPVELLRRLGADRTVVVVSPANRAEIERALGDQVEYVEQASPNGTGGAVAESAKLLQAKLLQKKDVQNPEHLLVLGSDSPLLKEETLRQLMAHHLEDGLALLGFRAADPTGYGRLIVEGDKLVAIREEKDATPDERAIGLCNSGVMAFDAALALDCLARIGNANAKGEYYLTDVVGIARAAGLGVAAIEAPEAEVLGVNSRGELAAVEALWQERRRAAAMAEGVTVVDPATTVIDATVTYEPDAIVHPGTQLLGTTHLAEDAEVGPNTRLTDTTVHAGARVDNSVADRAEIGPGSTVGPYAYLRPGTSLGAKAKAGTYVEMKNATIGEGTKVPHLSYVGDATIGDHTNIGAASVFVNYDGVAKHHTTIGSHCRTGSDNMFVAPVTIGDGVYTAAGSVITKDVPPGSLAVARGQQTTKPGWAAKFRAMKAAKKAK"
  },
  {
    "reference": "MSDRNELHGRHIVLGLTGGIACYKSAELVRLLVKAGATVQVVMTEAAEAFITPVTFQALSGRPVFTSQWDARPDNNMAHINLGREADAIVIAPASADFMARLATGRADELLSLTCLARPREHTALLVAPAMNREMWSHPATQRNVATLQADGTVILGPGCGEQACGETGDGRMLEPEALRDAVIAHFQPKVLAGRKVLITAGPTFEPIDPVRGITNHSSGKMGFALARAAQEAGAEVTLVAGPVHLPTPRGVRRIDVMTAQQMHDVVLPAAAQHEVFIATAAVADWRPATQSDQKIKKEGKKVAPTFALTENPDILAAVAALPGAPYCVGFAAESEQLLTHARAKLQRKRIPLIIGNLGPATFGRDENSLLVVDADSHHALPADGSQADKLTLARILMQELSQRLSNRTA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be able to bind CTP ligand in a variety of conditions2. The protein design should consider the endogenous proteins and enzymes present in the CoA from (R)- pantothenate: step 3/5 pathway to avoid interference with native metabolic processes.3. A key design requirement for the protein is the optimization of the Proton donor to achieve high catalytic efficiency for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. A protein with phosphopantothenoylcysteine decarboxylase activity, phosphopantothenate--cysteine ligase activity is needed to enhance pantothenate catabolic process in a specific context.5. Mg(2+) is required for the proper folding and stability of the protein.6. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal domain.7. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A The designed protein sequence is ",
    "response#1": "KRVLLGVSGGIAAYKAAELVRLLRAQGAEVRVVMTESAVQFVSPLTFQALSGNPVYISLTEVDAGNAMGHINLARWADVLLIAPATANIMAKIAHGLADDLLSTLYLAATCPVYVAPAMNQAMWSKPVTQENIAKLRQHGVGFIGPEQGSQACGEIGLGRMSEPADILALADRVLSAQDSLAGLRVLVSAGPTYEDIDPVRFIGNRSSGRMGFAVAEAAASAGAQVTLVAGPVSLPSPAGIARRLDVRSAAQMREAVLGAAASADIYIGAAAVGDYRPAQVASRKLKKRDGAPLVLDLAENPDILKDLAAASPHAFLVGFAAETHDVAAYAQDKLARKGLDMIAANRVGEGLGFEADDNALSLYWADGQRELPRADKQALARQLVAEIARRYDEKNTP",
    "response#2": "KRVLLGVSGGIAAYKAAELVRLLRAQGAEVRVVMTESAVQFVSPLTFQALSGNPVYISLTEVDAGNAMGHINLARWADVLLIAPATANIMAKIAHGLADDLLSTLYLAATCPVYVAPAMNQAMWSKPVTQENIAKLRQHGVGFIGPEQGSQACGEIGLGRMSEPADIVAMLEAALELAGRRVLVTAGGTHEPIDPVRFLGNRSSGKMGYALAIEARNRGASVILVSGPVALPPPYGVEMQQVETAIQMRNAVHRFITDVDVLVMSAAVADFRPDEVATQKIKKHSDEVQTHDGKLSLHLVPNPDILGELADTRNGSLEDSGRRLIRVGFAAETQQVVDYAQSKLHSKGLDLLVANDVSRSDSGFGTETNKVLRDDAGDWVELEGSKASVAAAILDRLRAERS",
    "response#3": "KRIVIGVSGGIAAYKAAELVRLLVREEALVQVAMTANAARFVSPLTFGALSGNRVVSHMFDEAAGAMDHISWGQDSDLIIIAPATANVIAKIAHGIADDFLTTMTLAATARILVCPAMNTRMYENQATQENLAVLQRRGIFVMEPGAGQLACREEGPGRLPEPAEIVDFALAALTRPWRGRHVLVSAGPTLEALDRVRVLSNRSSGRMGVLLAQAAHLRGATVDLVHGPLQVPPSWLEGLQCHAVVGSAAMDDRIQQLQPKADAVLMCAAVADLRRTSPTDVEKLPKDQLIESLEGGWEPVPDLLQSLVSRRPAGQSVLGFAALTGTDEQLLELGRRKFQAKGCDLLMVNPVDRIGQGLDSDQNAGWLLGADGSVHALGTAPKRQLARAVVAHAARLLTS"
  },
  {
    "reference": "MATPTSQTALRVNVYKFSRGRVGRERGCRSAMSTVMRESPRTMASRSSRPGSQPMRMRVGIHAPRDSLFSWSSGFDKFEGFVNWGGLILMLGGLRLFLENVIKYGVRINPLSWLGWLASEQVNDSYFHTPLFILATNIHILFAFFLEHLLARDVIPNNLGAYIHATHLTTILSIPIVILGLWTDLFSLLGRTIICAVYTVLFLKLWSYAQVNHWCRSERTWKKKFSRRRSFSFQKLLRKEYAEKINEQEEEAVALNLQQYPDNLTLGDLYYFLVAPTLCYELNFPRSQRIRKRFLLRRMLEVAAITNILLAMFQQWIIPSVRNSFQAFSDLDFIRCAERLLKLAIPNHILWLCWFYLTFHSFLNTLGELLRFADRNFYQDWWNAKDVGTFWRLWNLPVHKWAVRHIFVPMVKNKYKKSTASIVVFVLSAVLHEYLVSIPLHMYKIGVFLGMMVQVPLVYISTWVESRFGQRWGNMIVWASLILGQPLGIMVYYHDYVVKTFEPSMLEI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that can modulate the overall flux of metabolites through the Lipid metabolism pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "IMSATQTTVPESEKAVGGHLREVGLTFHFFNQLPAIIADNVGNSLAEAFEPIGIKDWNNIFWVAHPGNWAIMDAIETKLGLEQSKLATARHVFSEFGNMQSATVYFVMDELRKRSAAENRATTGDGLRWGVLFGFGPGISIETVVLQSVR",
    "response#2": "IMSATQTTVPESEKAVGGHLREVGLTFHFFNQLPAIIADNVGNSLAEAFEPIGIKDWNNIFWVAHPGNWAIMDAIETKLGLEQSKLATARHVFSEFGNMQSATVYFVMDELRKRSAAENRATTGDGLRWGVLFGFGPGISIETVVLQSVV",
    "response#3": "IMSATQTTVPESEKAVGGHLREVGLTFHFFNQLPAIIADNVGNSLAEAFEPIGIKDWNNIFWVAHPGNWAIMDAIETKLGLEQSKLATARHVFSEFGNMQSATVYFVMDELRKRSAAENRATTGDGLRWGVLFGFGPGISIETVVLQSVV"
  },
  {
    "reference": "MGSACKDGREEVIQAWYMDDSDADQRLPHHREPKEFVSLDQLTELGVLSWRLDADNYETDEELKKIREGRGYSYVDICVVCPENLPNYEEKIKDIFFEHLHTDEEIRFCVAGSGYFDVRDRNDAWIRVWLKKGGMIVLPAGIYHRFTLDTENYIKIMRLFVGDPIWTPYKRPHDHLPARKEYIEAFVQKGGANHAVDATGSVKEEFPVVSLAYPMAVPKPMEGLNDTGPPPFLTKTYDLVDDPNTDPIISWGRGSNSFVVWDPQAFAMNLLPRYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEWFIRGQKHLLKNIRRRKSSSHAQTSQQVLDPCVEVGWFGLDAEVDRLRRDKQTKGTEMKQQQMMGFLARAIQNPSFIQQLLQQKERRKELEEAISKKRRRPIEQGPCDIGIGELDQIGEERTYVKLEPFDYGDISEFDVVELEKLAEDMQGPSGTSKNMEEEYVEKEEKHDSGDKALDMGFWEDLLNEEIEEEIGLLVGEGEEEDIGMLAEQLGILGSSPR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should take into account the physiological conditions of the L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6 pathway.2. For general function, the protein need meet that Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site3. The protein design must be able to perform acireductone dioxygenase [iron(II)-requiring] activity, acireductone dioxygenase (Ni2+-requiring) activity in order to contribute to L-methionine salvage from methylthioadenosine.4. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.5. The protein should be designed to catalyze the following reaction 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-   methylsulfanyl-2-oxobutanoate + formate + 2 H(+). The designed protein sequence is ",
    "response#1": "MSVLSGTRIIPLLGPTASGKTDVAVALAEHVPVEIINADSMLVYRGLDIGTAKPSPEERAGVPHHLLDIWPVTEPASVADYQRLAKARIAEIRGRGKLPVFVGGTGLYIQALLYGFEFGPGPDPEIRAELEAEAVRIGPEALHARLAAVDPVAAARILPGNGRRIVRALEVIELTGKSFTAELAAGKPFDETRPWKLLKLTLDRPVLYDRIALRVDRMWADGLVAEVQALLARGIPGGGPASRALGYQELISHLRGECSREEMAEKGVIATRQYAKRQSTWFRAYPEIAWYDVDDPEALALVRRYL",
    "response#2": "MTTNPDRISTPATLTAETLAAYRRDGFLVLPGFFTPEEVATAQERIWGIVDGKDPGDMTTRNVMTLDRSNPACRQLATHPKVLGAVQAVLGSHIRLWHDQAIYKEAYPGTGAETAWHQDFPYFPLNGPRLLNVWVALDDATLENGCLWFIPGSHRWNDFMPTPAVEGQNYSQDVAAAVPAHEAKAVPMRAGGVSFHSGLTVHASGANTSDRPRRGLALSYMGADAVPLADGNRDATAVRGGANLVHGDRALPFPVVHGRP",
    "response#3": "MSRSSGDATHIAEPRTAARTRAELIADVRARGLTGVRAVEPADLAHHRADAGAALAAALADGHGYPCVVKPARGAASVGVVRVDSAADAVAAFREAAGFAGDDLLAQELVDGPEYAVDLFAHEGDVVTRICRKHMRLSGEGGTPVTVDVTVADPAVRALATDVLRQLGLRYGPAHVEVRRTPDGPRVIEVNPRIAGGLHVLGASARVNLPALALRAATGEDVRLPEPAAHGAPARKFVQFHLPVTPVRPLDGVLTAEQAAALPGVVDVRPILKRGDIVPGVHDTSDRVGYVLATGDSAAAARAAAERAAAWLTVTVRP"
  },
  {
    "reference": "MKLAALMDRAGLGSDGAGDTQVTGFAIDHRKVAPGTVFGAFRGAQFNAEDVIADAVTSGAVAVVARAEAQVEGALHVASEEPRQTFAQIASGFFSPVPETIVAVTGTNGKTSTAEMTRQIWRMCGESAASIGTLGVTTPDGSVSTGLTTPDIVTFLSNMSGLKREGVTHVAYEASSHGLSQYRNEGVPVSACAFTNFSRDHLDYHGTMEAYFEAKMRLFNEVIEPGAPIIIWRGADSNEWTAKAIEHAQKCGNPLHVVGEASQHGADPFLRLVNRVPTQLGQTLTIEHSGEESTVKLPLIGAYQAANAMCAAGLALATGSNKAQVFDALSRLQPVRGRLERAVITQKGAPVYIDYAHTPDALEAAIAALRPHVDADKGGRLIVVFGAGGDRDSGKRAPMGEVAAKSADLVIVTDDNPRGEDPSHIRGEIMGGALGIDGADVRNIGDRRRAVSAAIEAAEAADIVLIAGKGHEQGQIIGSGENMRVLAFDDVQVARECAAEAGKALS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a high affinity for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate.2. The protein should be optimized for activity in Cell wall biogenesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. A protein that can perform magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity in cytoplasm to support regulation of cell shape, cell wall organization, cell cycle, peptidoglycan biosynthetic process, cell division.4. The designed protein must have at least one Mur ligase C-terminal domain.5. The protein should have a fast turnover rate for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "MQLATLLNHLHPIIHQSDLSNTKIIESVVADSRQVKPFSCFVAIPGTTVDGHEHIPGAVRSGAAAIVCERMPTEEERTDQVIYIQVENTREALGHLAANFYNHPSAELFVVGTGTNGKTSTAWWTAQALSALGRRCGVIGTLGVGEPGAVDATGLTTPDPVTLHAAFKRFADTGFAACALEASSIGLDEHRLDAAPIDVALFTNFTRDHLDYHGSMDAYWAAKRQLFAWPGLRAAALNIDDPQGALLAGELEGGALDLWTVAVDAPARLRAADVRYVDGGLAFGLCEDDTTVPVRSTLIGDYNVHNLLVVSALVAQGVPFDKALQALCSFQGVAGRLEQVETGRDFHVVVDYAHKPDAVEAALSTLRPLTAQAGGRLICVIGAGGDRDTGKRPVMGEIAARLADLVIVTDDNPRSEDPAVIRAAVLEGTRDGAGEVREVGDRREAIRVALSSAGPGDIVLVAGKGHEDYQIIGKTKYHFDDREELRNAYEHTR",
    "response#2": "MKLQELLKGVKILKIVGNDQLELRTDSRRVQPGDAFIAWPGYATDGRRFVRAALDAGATTCLVEAEVAKDYPQIEVIQVKDTRRALALMSAKFYAEPATQMKLVGITGTNGKTTTSYITRAILREAGYKVGLIGTIQIMIEDEVLPIHNTTPDVVELQHTLAMMRDKGMDYVVMEVSSHALDQNRVAGIEFDTAVFTNLTQDHLDYHKTLENYKLAKAKLFDHVSEPGTKQGKTAVVNIDDAASETMLAHAKCAHLTYAIEKEADLRATDILVHANGAEFTLNHASFGKMPLKLHITGIFNVYNVMSAVGAAVGMGVDLQTALTGMEHITMVPGRFQYLVSDEKITAIVDYAHTPDALKNVLETINNIRTKNEELITVVGCGGDRDKTKRPKMGHIASALSTKVIFTSDNPRSEVPDAIIEDIEKGVEPQNYKKTLSIVDRKQAIKTACQMAQPNDIILIAGKGHETYQEIKGERYDFDDVEVAKRAVKKLKKTA",
    "response#3": "MTEIENICWDSRRVQLNSLFICVKNKNVDRHDYAASAIDNGAIALVIEHDIEKVSKNITIIKVQDSKKAMAKIASLYYGHPSQRMMLVGITGTNGKTTTSYITRAILREAGYKVGLIGTIQIMIEDEVLPIHNTTPDVVELQHTLAMMRDKGMDYVVMEVSSHALDQNRVAGIEFDTAVFTNLTQDHLDYHKTLENYKLAKAKLFDHVSEPGTKQGKTAVVNIDDAASETMLAHAKCAHLTYAIEKEADLRATDILVHANGAEFTLNHASFGKMPLKLHITGIFNVYNVMSAVGACLAMGLPLEVIQRGLERVPQIPGRMELFQSEGKAKVVVDYAHTPDALEKALKALRVHCKGKLWAIFGCGGDRDAGKRPMMAEIGERLADKVILTDDNPRSESPQAIVKDMLAGMNYPNLAVVEHERYKALQYALSQGDESDIILLAGKGHEPYQEIKGVKYPFDDKQVVEELLG"
  },
  {
    "reference": "MEIASQEDPIPINTSYGNSGGGHGNMNHHHHANSAPSSLNITTSNPLLVSSNSNGLGKNHDHSHHHHVGYNIMVTNIKKEKPVVIKYKECLKNHAATMGGNAIDGCGEFMPSGEEGSIEALTCSVCNCHRNFHRRETEGEEKTFFSPYLNHHQPPPQQRKLMFHHKMIKSPLPQQMIMPIGVTTAGSNSESEDLMEEEGGGSLTFRQPPPPPSPYSYGHNQKKRFRTKFTQEQKEKMISFAERVGWKIQRQEESVVQQLCQEIGIRRRVLKVWMHNNKQNLSKKSNNVSNNVDLSAGNNDITENLASTNP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must have high specificity towards protein homodimerization activity, DNA-binding transcription factor activity. The designed protein sequence is ",
    "response#1": "GLQGGHLRIASFRSAATHVLPPRLALFRQRCPGVSVSIIETDPQGVEQALREGKVDIGLLPLPRSEEFDTWEITRDEYVVLLPSTAHPLGQPLTWAQLSRYEFILYNYAECTTAVRQHWATARQELKVAYEIKEDSTIVSMVAQGLGAAILPRLAAVPIPPEVAAHSLPIPLERVIGAAILASALQVPSVFAFLDVLRQS",
    "response#2": "GLQGGHLRIASFRSAATHVLPPRLALFRQRCPGVSVSIIETDPQGVEQALREGKVDIGLLPLPRSEEFDTWEITRDEYVVLLPSTAHPLGQPLTWAQLSRYEFILYNYAECTTAVRQHWATARQELKVAYEIKEDSTIVSMVAQGLGAAILPRLAAVPIPPEVAAHSLPIPLERVIGAAILASALQVPSVFAFLDVLRQAA",
    "response#3": "GLQGGHLRIASFRSAATHVLPPRLALFRQRCPGVSVSIIETDPQGVEQALREGKVDIGLLPLPRSEEFDTWEITRDEYVVLLPSTAHPLGQPLTWAQLSRYEFILYNYAECTTAVRQHWATARQELKVAYEIKEDSTIVSMVAQGLGAAILPRLAAVPIPPEVAAHSLPIPLERVIGAAILASALQVPSVFAFLDVLRQA"
  },
  {
    "reference": "MTKKLHIKTWGCQMNEYDSSKMADLLDSTHGYQLTDNPEEADVLLLNTCSIREKAQEKVFHQLGRWHMLKEARPELIIGVGGCVASQEGAHIRERANYVDIIFGPQTLHRLPEMINHVNGTRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCSFCVVPYTRGEEVSRPCDDILFEIAQLADQGVREVNLLGQNVNAYRGASYDGGICTFAELLRLVAAIDGIDRIRYITSHPIEFTDDIIDVYRDTPELVSFVHLPVQSGSDRILTMMKRAHTALEYKSIIRRLRKARPDIQISSDFIIGFPGETQQDFEQTMQLIADVNFDMSFSFIYSARPGTPAADMVDNVSEEEKKQRLHILQERITQQAMQYSRRMKGKVQRILVEGTSRKNVMELSGRTENNRVVNFEGAPSMIGKFVDVEIVDVYPNSLRGVLLRTEDQMTLRSHESTASVIARTRKENELGVGMYQP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of Radical SAM core, TRAM domains is critical for the interaction of the designed protein with its intended targets.2. The designed protein must be optimized for metal ion binding. The designed protein sequence is ",
    "response#1": "EEGRNTLCISTQVGCPVKCAFCRTGASGFNRNLSVSEIIGQVWRAASRVGFSQNEEQKPISNVVMMGMGEPLYNVDAVLKVTEILLNDNAFALSKRRVTISTSGVAPIIDKIAGKVDVALALSLHAPNDELRDVLVPLNKKYKIDVVLKSVRNYLSKSNANCGKATIEYVLLDHINDSTDQAEELARLLKDTPCKINLIPFNPHEQSEFKRPSNSRVDRFYKVLTGHGYTVMTRTTRGDR",
    "response#2": "ISTQAGCPLKCSFCATGTMGFKRNLTKAEIFDQFFLLNKLSLEKFEKPLSNLVLMGMGEPFLNLENVVDALTIISSPEGLNFSSTRITISTIGIPMLIEEYAEKNYPWNLALSLHSSISEVRKTIIPASTKYPLDELQEALKRYSRMRKKPITVEFLLLRKVNTSK",
    "response#3": "STLLSIKTGGCEEDCGYCSQSAHHDTGLKAEKLMEVDAVLAAARTAKENGATRFCMGAAWRNPKDRHIEPIKEMIRGVKDMGLETCVTLGMLEEHQAKALAEAGLDYYNHNLDTSPEFYGQIISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLIAQLANMNPYPESVPINNLVAIEGTPLENAQALDPFEFVRTIAVARITMGGS"
  },
  {
    "reference": "MKTNPIKLEKKLKLKFSDQKIFIKSLTHKSFDSINNNEKIEFLGDRVLGLIIAKKLLELYPDEKEGVLDKKFASLVNKKKCLEIAKKIELEKYILVLNPKNKKIEIEDKIVADCLEALIGAIYLDKGLNFTERFILNLWSEHITASVITQIDAKTKLQEYSLKIFKVLPIYKLISNTGPRHKPLFKVAVKLKNTKFFTAEGTSKKDAEQNAASLCLQDIFKK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must have a active site that is structurally stable and resistant to denaturation.2. The protein contains novel DRBM domains that confer a unique function or activity.3. The designed protein should have metal ion binding, rRNA binding to facilitate mRNA processing.4. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.5. The protein must exhibit the following characteristics: Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.; Digests double-stranded RNA. The designed protein sequence is ",
    "response#1": "MDGVDELLLRKFNLTLPPGLLRTAFVHKSFSFENGACQNNERLEFLGDAVLGLVVSHYLFETCPEYTEGQLSAARSYIVSGTSIAQIAKELNLGQFMLLGKGEKLAGGMDKTSLLADLLESLIGAVMVDQL",
    "response#2": "IERCQEILGYDFRDLDLLRSALTHASGASHRLASNERLEFLGDSVLGLTVCEWLFNEYPEYSEGDLTKIKSAVVSRRSCGKVACKLGLDQCLIVGRGVTRNRSYPKSLVSDVFEAVIAALYIDGD",
    "response#3": "RLLITALTHPSYRNEFPSAEEDSERLEFLGDAVLGLVVTEHLFLLFPALNEGLLSTTRAALVNAEACFEYTQKLSLGEHLLIGRGEKMQSHRGKISAYANLLEAILGAVYLDGGLSPARQIIVPLLPDKESILF"
  },
  {
    "reference": "MGLDKVVKDIMDKAEADSRDITAKAAAEAAEIKKSAEAEAKQIIAAENARAEQAISKMRQRELSSAKLDVKKAKLNSEKDVLAETHEAFVRQLSTLPREKKADLLQKLVKLAKKDIPQGKIFTNAADADLVKDSGYEYGGNVKCIGGIVVTSVDGSVNLDYTFDSILEDVWTSSMKPVSDILFGSR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that can perform proton-transporting ATP synthase activity, rotational mechanism, ATP binding in proton-transporting two-sector ATPase complex, catalytic domain to support proton motive force-driven plasma membrane ATP synthesis. The designed protein sequence is ",
    "response#1": "GQRVFDTFFPLGMGGTAAIPGGFGAGKTMTQHQLAKWSSADIIVYVGCGERGNEMTDVLEEFPKLEDPSTGKSMMERTVLIANTSNMPVAAREASIYTGITIAEFYRDMGYNVALMADSTSRWAEALREISGRLEEMPAEEGFPAYLPSRLAEFYERAGYVKTLGQDREGSISLIGAVSPPGGDFSEPVTQNTKRYVRCFWALDKSLASARHFPAVNWLESYN",
    "response#2": "VNPLDRDREWTVSVKVKLGDRVKPGQVVAEVPETSIVTHRVMVPPHLSGEVVEVVPNGKYTVDQEIVTVKDDKGNNHQIRLHQKWPVV",
    "response#3": "GQRVFDTFFPLGMGGTAAIPGGFGAGKTMTQHQLAKWSSADIIVYVGCGERGNEMTDVLEEFPKLEDPSTGKSMMERTVLIANTSNMPVAAREASIYTGITIAEFYRDMGYNVALMADSTSRWAEALREISGRLEEMPAEEGFPAYLPSRLAEFYERAGYVKTLGQDREGSISLIGAVSPPGGDFSEPVTQNTKRYVRCFWALDKSLASARHFPAVNWLESYSXI"
  },
  {
    "reference": "MGKYYCDYCDVFLVSESPSVRKAHNSGRNHLTNVRDYYSSLGHDKAQSYIDEITRMFETGGGNSTSNRGPGGNPPGSQPGPPNAGMSGPMRPPFSNSTAGPNMPPLPPAMLALMNGQNGMSSPGSGPPPMRFAGPPIPNNMPPGMMQPPHVNGYSSGPLPPQPQPASGGQGAPPLTARMNPDRARQLG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should be able to enhance the efficiency of mRNA 5'-splice site recognition.2. For general function, the protein need meet that U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing The designed protein sequence is ",
    "response#1": "RSHVSPIISNLTMDNLQESHRDLFQVNILIGRNIICKNVVDFTLNKQNGRLIPALSALIALLNSDIPDIGETLAKELMLMFVQQFNRKDYVSCGNILQCLSILFLYDVIHEIVILQILLLLLEKNSLRLVIAVMKICGWKLALVSKKTHDMIWEKLRYILQTQELSSTLRESLETLFEIRQKC",
    "response#2": "RSHVSPIISNLTMDNLQESHRDLFQVNILIGRNIICKNVVDFTLNKQNGRLIPALSALIALLNSDIPDIGETLAKELMLMFVQQFNRKDYVSCGNILQCLSILFLYDVIHEIVILQILLLLLEKNSLRLVIAVMKICGWKLALVSKKTHDMIWEKLRYILQTQELSSTLRESLETLFEIRQKC",
    "response#3": "MPKYYCEYCDIYLTHSSPVGRRQHIQGRKHISAKIEYL"
  },
  {
    "reference": "MYAHRLQFFLLHAWWVLLQAGAATVAQVPLRTLGQPSSPSPLAYMLSLYRDPLPRADIIRSLQAQDVEVDGQNWTFAFDFSFLSQEEELAWAELRLQLPSPVDLPAEGQLTIEIFHQPKLDTEKDPGNCLRRLRMELFTITLSQVTFSSGSMVLEVTKPLSKWLKDPRALEEQMSSLSRECWQPSPMPPVTGTSVLLMLYSNLPREQRRLGGATLLWEAESSWRAQEGQLSRERVLRHRRHHLPDRSQLCRKVKFQVDFNLIGWGSWIIYPKQYNAYRCEGQCPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLEHHKDMIVEECGCL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that contributes to digestive tract morphogenesis, transforming growth factor beta receptor signaling pathway involved in primitive streak formation, cell migration involved in gastrulation, positive regulation of epithelial cell proliferation, nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry by functioning in extracellular space with type I activin receptor binding, growth factor activity.2. The protein must contain a signal peptide for proper functionality.3. The protein contains novel TGF-beta family profile domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "GCPVCVWRQHSRELRLESIKSQILSKLRLKEAPNISREVVKQLLPKAPPLQQILDLHDFQGDALQPEDFLEEDEYHATTETVISMAQETDPAVQTDGSPLCCHFHFSPKVMFTKVLKAQLWVYLRPVPRPATVYLQILRLKPLTGEGTAGGGGGGRRHIRIRSLKIELHSRSGHWQSIDFKQVLHSWFRQPQSNWGIEINAFDPSGTDLAVTSLGPGAEGLHPFS",
    "response#2": "NLCKWCEVSSPVCKDNVCLSNCNLSSFCTVMEEICIAIWRKTNYTVTVHTMCHHQTLPLEGVDPGLLLNFTTRECHMVQQPAEEGTMMVCGCHGEHECNDKLIFEKGANGFC",
    "response#3": "NLCKWCEVSSPVCKDNVCLSNCNLSSFCTVMEEICIAIWRKTNYTVTVHTMCHHQTLPLEGVDPGLLLNFTTRECHMVQQPAEEGTMMVCGCHGEHECNDKLIFEKGANGFQ"
  },
  {
    "reference": "MAKITIAQQLATTLEQAGVRRIWGLTGDSLNGLSDALRKMDSIRWMHVRHEEVAAFAAGADAAATGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAQIPSSEIGLGYFQETHPQELFKECSHFVELVSNPAQMPQVLHRAMRAALLKRGVAVVVVPGDVALQEVEDDLKPWPALAAPRTLPAPHDLDQLVRLLSHGKAVTLLCGSGCAGAHDQVVALADALGAPVVHALRGKEHLEWDNPFDVGMTGLIGFSSGYHAMLNCDTLVMLGTDFPYRQFYPGQATVVQIDRNPEALGRRVPLDLGIAADVGETLAALLPRLPYNGDRRFLEESLEHYRKARQGLDDLARPAADDRPIHPQYLTRLLSELADDDAIFTADVGTPTVWAARYLKMNGRRRLLGSFNHGSMANAMPQAMGAQAAFPDRQVISLSGDGGFSMLLGDFISLAQLRLPVKIIVYDNASLGFVAMEMKASGYLDTGTDLHNPDFAAMANAMGILGIRVEQSNALEGALRTALAHDGPVLVDVVTATQELAMPPAIKLEQAKGFSLYMLKAVMSGRGDEVIELAKTNWLRGMVERTLR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of thiamine diphosphate is crucial for the protein to function optimally under specific conditions.2. The designed protein should be involved in pyruvate catabolic process by localizing to plasma membrane and exhibiting magnesium ion binding, ubiquinone binding, lipid binding, thiamine pyrophosphate binding.3. The protein should have a fast turnover rate for a ubiquinone + H2O + pyruvate = a ubiquinol + acetate + CO2.4. The presence of Thiamine pyrophosphate enzyme central, Thiamine pyrophosphate enzyme N-terminal TPP-binding, Thiamine pyrophosphate enzyme TPP-binding domains is critical for the interaction of the designed protein with its intended targets.5. The protein should have a high affinity for FAD, thiamine diphosphate.6. For general function, the protein need meet that It channels electrons from the cytoplasm to the respiratory chain at the cell membrane via ubiquinone.; A peripheral cell membrane enzyme that catalyzes the oxidative decarboxylation of pyruvate to form acetate and CO(2) The designed protein sequence is ",
    "response#1": "MTVADLLAKTLASAGVSRIWGVTGDSLNGLNDSLRRFDQIEWMHVRHEETAAFAAGAEAAITGKLAVCAGSCGPGNLHLINGLFDCHRNRTPVLAIAAHIPSSEIGLGYFQETEHPQELFRQCSHYVELVSNPERFPELMRRAIQAEARSGPVSVVVALPGDLLFGKAAPMAAHAWQAPPPRVVPSAEELAQVAALARMLNDCSRVTLLCGGGCAGAHDEVVALAAALKAPVVHALRGKEHVEYDNPYDVGMTGLIGFSSGYHAMKECETLLMLGTDFPYRQFYPTEARIAQVDLRGEVIGRRCRVDVGVVGDVKLTLMPLLERHLQRRGLKQLAQDRQRYLEARKGLDQRATPAPGRPIHPQYLARVLDEVAAEDAVFTCDVGTPTVWAARYLTMNGRRRLLGSFWHGSMANAMPQAIGAQAASPGRQVVSLSGDGGFAMLMGDLLTLRQVGLPVKIVVVNNGTLGFVELEMKAGGFLDTGTTLRNPDFAAVAEASGLLGIRVEDPADLREGLQRALRHDGPALVDVVTNRMELAMPPAIQLEQAKGFTLYMLRAILSGRGDEVLELARTNLR",
    "response#2": "TVADTIVQIIREAGVQRVYGIPGDSLNGFTDALRRSDGITWEHVRHEEAAAFAAAAEAALTGELAVCAGSCGPGNLHLINGLFDANRSRVPVLAIAAQIPGPEIGSTYFQETHPQDLFREASVYTELVSYPEQLPRILEIAMRTAIERRGVAVVVIPGEDALGTPCASILARDATEILNSAAKVTILGGAGCNGAHKELIATADALKAPIVHALRGKEYIEYDNPFDVGMTGLLGFSSGYHAMEDCDVLLMLGTDFPYPQFFPSHAKIIQVDLRGNQIGRRTKVDLGLVGSIKDTLSAILRVLHRDTTPEDLLERLAALLNGAGRVTILAGRGCRMAHAALVTLAERLQAPVVHALGGKEFVEYDNPYDVGMTGLIGFSSGYDAMMGCDTLLMLGTDFPYRQFYPTKAKVAQIDLRPENLGRRTGIDIGLVGDVGPTIQALADTLGTPLTPETELAKVLTTSKKITILAGAGCAEAHEELIELAGRLKAPVVHTMRGKEFVEHDNPFDVGMTGLLGTSSGYQAMMTCELLLMIGTDFPYQQFYPRDATIVQIDRRGEQLGRRTKVDYGFVGDAKTTLLAKLLSPTSSPEDLASARAVRILDGAKKVTILAGAGTQGAHDEVVALADRLAAPIVHALRGKEFLEYDNPYDVGLTGLLGFASGYRAMDAADAVLMLGTDFPYPQFYPDHAKTIQVDIRGSQLGRRHPLDLGLVGDVKATAAAILRVLHRDTT",
    "response#3": "TASGYLVEALEKAGVKRVYGVVGDSLNGFTDALRRRKSIDWIHMRHEEAAAFAAGAEAHLTGSLAVCAGSCGPGNLHLINGLFDCQRNGVPVLAIAAHIPSSEIGIDYFQSTHPENLFRDCSHYVELVSNPKQLPQILKRAIRVAIAKRGVAVVVLPGDVALMPDHDQLAGRLRDATEILNSAAKVTILGGAGCNGAHKELIATADALKAPIVHALRGKEYIEYDNPFDVGMTGLLGFSSGYHAMEDCDVLLMLGTDFPYPQFFPSHAKIIQVDLRGNQIGRRTKVDLGLVGSIKDTLSAVTAVLEHRGAPDADISRAADLLNAGRRVTILAGAGTAGAHDEVVALADALGAPIVHALRGKEFIEYDNPFDVGMTGLLGFASGYRAMDRADTLLMLGTDFPYRQFYPKHATTIQVDIRGEQLGRRHPVDLGLVGSVKDTAEAAALARMLNDCSRVTLLCGGGCAGAHDEVVALAAALKAPVVHALRGKEHVEYDNPYDVGMTGLIGFSSGYHAMKECETLLMLGTDFPYRQFYPTEARIAQVDLRGEVIGRRCRVDVGVVGDVKLTLLDVLFPAPGRDVGSPVVWASRYLTMTRERRLIGSFAHGTMANALSHAIGAQAAFPDRQIVALAGDGGLAMLLGELITIVQNDLPVKTVVFDNSSLNFVELEMKAAGFVTYGTDLRNPDFAAVAEAIGIRSFRVDESTDLETSLAAALAHPGPALVSSVLLQPG"
  },
  {
    "reference": "MDLIKAVESQQMRKDLTPFNVGDTIRVHYKVIEGDRERIQPFEGIVIKKSGSGLRETFTVRRVSYGVGVERTFPLHSPRIEKIEVIRRGKVRRAKLYYLRKRVGKAATKIKELM",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have structural constituent of ribosome within ribonucleoprotein complex, ribosome to regulate translation.2. For general function, the protein need meet that This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site. The designed protein sequence is ",
    "response#1": "IGCMVTLRGDRMFEFFDRLVNVALPRVRDFRGVSSKSFDGRGNYTLGIKDQLIFPEIDYSKVDKTKGMNICITTTARTDAEGLALLRTMGMPV",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#3": "PVGIMVTLRSDRMYAFFDRLINLALPRIRDFRGVNPKSFDGRGNYSLGLREQLIFPEISYDSIDQMRGMDVSIITTATTDEEGRALLKVMGMPV"
  },
  {
    "reference": "MGPDPSLAYRPECHEKDKTEFRNFENGDLFDRVFNTYKLMHTHQTLDFVKQKHQVWSNCSHFSLSMMDSIDSLDELVDESDPDVDFPNSFHAFQTAEGIRREHPDKDWFQLVGLIHDVGKVMALYSEPQWAVVGDTYPVGCKFQNSIVFRNSTFEGNPDGKNPAPNTEFGIYEPQCGLDKVLMSWGHDEYLYRVMKFNKCTIPEEGLYMIRFHSFYPWHSNGDYMHLCNEKDQQMLPWVKEFNKFDLYTKSTELPDVERLKPYYQSLIDKYCPGVLQW",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECQ",
    "response#2": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECR",
    "response#3": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECN"
  },
  {
    "reference": "MTKAYNVAILGATGAVGTRLIEQLEQSTIPVNSLKLLASPRSAGKQLEFKGEKITVEATTPDSFDGIDLVLASGGGSVSAQYAPEAVKRGAVVVDNSSYWRMNDDVPLVVPEVNESALSSHNGIIANPNCSTIQMVVALAPIKQQFGLKRVIVSTYQAASGAGQSAWNELVAETTAYNAGEPVHANILPVKGAQHHYPLAYNLLPQIDVFEDDGYTHEEWKMIHETKKIMFGDKNSKAVKVTGTAVRVPVPIGHGETVYFETNDGAGATTASIQAALRSAAGVVLEDNPAEQLYPQPVNAAGKRETFVGRVRPDLENDDSFHMWVVSDNLLKGAAWNTVQIAERLVALNLVRVPQGASDKFPVTK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design must be able to perform NADP binding, aspartate-semialdehyde dehydrogenase activity, protein dimerization activity in order to contribute to threonine biosynthetic process, 'de novo' L-methionine biosynthetic process, lysine biosynthetic process via diaminopimelate, diaminopimelate biosynthetic process.2. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.3. The designed protein should have a high specificity for substrate, NADP(+) binding, with minimal off-target effects.4. A protein should be able to manipulate the activity of enzymes in the L-threonine biosynthesis, Amino-acid biosynthesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "MANLAVVGATGAVGTVLLGILSARQDVWGEIRLVASARSAGRRLTVRGEQVEVVALSEEAFEGIDVAMFDVPDEVSAQWAPIAAAKGTVVVDNSGAFRMDEDVPLVVPEVNAAAARIDAITPEDKIVAVNPTCVRVPVLRSHLIPLHRAWGISRIHAATYQAASGAGQAAMDELAAGTRAYLDGTPFTPQVLPHPYAFNMFAHNSPWIEDEGYCEEELKMRNESRKMLHLDDLRVHATCVRVPVLRTHAEAINVSFRSPCEVAQAYELLRQAPGVKVLEDRAANRWPMPTDVSGGEEVVVGRIRRDPTLDHTLDMWVVGDQLLFNVHQTAAEGLFREGLVEWVSAQTYQAASGAGAQNMRELIAQMGTINSTVSDLLADPRSNILDIDSAVAQAMRGGDFPTDNFGHPLAGSLLPWIDTQLENGQSREEWKAQAETNKILGRSGAPIPVDGTCVRIGAMRCHSQALTIKLNKDVDIKEVESIIGSANDWVKLVPNDREVTLKELTPTAVTGSLHIPVGRVRKLNMGSQYLSAFTVGDQLLGS",
    "response#2": "MVIGVVGATGQVGRVMLTLLADRAVSHSAIRVFASARSAGTTVQYAGLPLTVEDAETADLTSEGRRVDIAIFSAGGSTSKALAPRFAEAGATVVDNSSAWRRDDEVPLVVSEVNPEKLANIPKGIIANPNCTTMAAMPVLIPLHRAWGISRIHAATYQAASGAGQAAMDELAAGTRAYLDGTPFTPQVLPHPYAFNMFAHNSPWIEDEGYCEEELKMRNESRKMLHLDDLRVHATCVRVPVLRTHAEAINVSFRSPCEVAQAYELLRQAPGVKVLEDRAANRWPMPTDVSGGEEVVVGRIRRDPTLDHTLDMWVVGDQLLKGAALNAVQIAELVAAEKL",
    "response#3": "MAKTGLVGWRGMVGSVLMGRMRDENDFANIEPTFYSTSNAGGEAPDVGKGAGVLQDAENIDSLKEMDVIISCQGGDYTKAVFPKLREVGWDGYWIDAASALRMSDDAVIILDPVNGDVISEQLAKNKGIKVAGTCVRVPVMSGHLAPLHNKYTVKRVVVSTYQSITGTGVKAVRQLENEYEGIRGEMAYPYPIHRNALPHCDVFEDNGYTKEEMKLVRETQKILNDNTIAVTATAIRIPVVGGHSESVNLQFENDFEIEDIRRILSETPGVTVQDNTDTNTYPMPIYAQGKDDVFVGRIRRDHSQPNSLNMWIVADNLRKGAALNSVQGLFREGLVEWVSAQTYQAASGAGAQNMRELIAQMGTINSTVSDLLADPRSNILDIDSAVAQAMRGGDFPTDNFGHPLAGSLLPWIDTQLENGQSREEWKAQAETNKILGRSGAPIPVDGTCVRIGAMRCHSQALTIKLNKDVDIKEVESIIGSANDWVKLVPNDREVTLKELTPTAVTGSLHIPVGRVRKLNMGSQYLSAFTVGDQLLNL"
  },
  {
    "reference": "MKIVTGAEMRRIDEATIQRFVTGIALMERAGQRVFEHVASMCAPLESATVSIVLGRGNNAGDGLVVARLLAEAGASVILLHLHRPEDLSPDAAKNHARLAQLRDGPRLSERFLYLGNWREAAASALEESDLAVDALLGTGLAKPVDGQYAEVVDLLNDGGTPIVSIDVPSGVNADTGEIMGTAVVADATVALGLPKIGCLFYPGKGCAGELVVGDIGIPREVVDEERLSVEGIDFETALADLPHHAPTAHKFARGSLLLVAGSRRYAGAAALAATAALRTGAGIVYCAGPESIRAIVQTAAPEVVFVSIAETASGSIAHRAAERLLDEVRFDALAVGPGLTADRETASFVPWALERCPKPILLDADGLNALAGKFDLVQRASRDKEIVVTPHSGELARLAGAGADGLPMERIERLRSLVAGTWVTIVHKGAPTVVAHHDGAASVNMGGHPGMATAGSGDVLTGVVAGFLAQGCGAARAARLGVYLHSRAADIAASELGERGMIARDCLEALPLALRELE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.2. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.3. A protein with NADHX epimerase activity, NADPHX epimerase activity, metal ion binding, ATP binding, ADP-dependent NAD(P)H-hydrate dehydratase activity functions is required to enable nicotinamide nucleotide metabolic process.4. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.5. The K(+), AMP binding site should be located in a region of the protein that is accessible to the ligand.6. The designed protein must have at least one YjeF N-terminal domain. The designed protein sequence is ",
    "response#1": "MRAMDAQTCKLHAMTGLALMERAGRAIFSKLSTTKPWCETRPLIVCGPGNNGGDGLVVAEALLLAGMDVSVCLVSEDQHLSTENRQVYERLKDRVHRIQRSEDIEWLKQQISCHDWILEALFGIGLTRPIEGVFKAVVALLNASGKSIVSIDLPAGLHTDSGQVLGMAVNATETWAIQAFKFGHFLADGKDYCGRLDCVDVGIVPVETPSRQRLCVLSGSAGKTGAAALVCEGALRVGAGLVTLFVPRSLNPVMEVKLTEAMTLPVEETEAQSVSLAARETILEFLADKEALAAGPGISLHEGTQALLKELVEKSPVPLVLDADALTILARDPAPLQNASSPVIVTPHPGEMARLAGLSVPQVQKNRLDVAAEFSRRHNVIVVLKGHGTLVAAPDGRLAVNTTGTPAMASGGMGDVLTGMIAGLVVQGLEPFRAACLGVYLHGLAAEEASGNATSRGLIATDLLPVIAPSLFVERFPSRSFASHKGTYGSLAVVGGDTGMCGAPILAARAALHTGAGKVHVAFIGEGAPPYDPPFPELMLHPPGTLGFGLEAMDALAIGCGMGHGPRAVSSLKQAIASDLPLLLDADALNLLASETALADALAARAVIDVDGNADAESGGTACACILTPHPLEAARLMGCDAAQVQRDRIGTARALAARFGAVVVLKGAGTVIASPDGRAAVNPTGNAGLATGGTGDVLGGIVGALLAERLPSYEAALAGVYLHGWAADTLTASGRGPAGLTAGELAPVVRDLLNRL",
    "response#2": "MRDIENRIFAGGMPVAALMEKVAGLIADRIQTKLAEDTTYFPHSPSFRIGILVGPGHNGGDALVVARELYFRGYEVGIYCPFSKRKELTSQHLQYAQSIGIICYESIELLADCHLFIDGLFGFGLERAISDPIATAINQLNEWHKPVIAIDLPSGLHTDTGEVLGTAVRATHTLCLGLWKLGLLQDQALDCVGKAELIDFDIPLADIAPSLFVERFPSRSFASHKGTYGSLAVVGGDTGMCGAPILAARAALHTGAGKVHVAFIGEGAPPYDPPFPELMLHPPGTLGFGLEAMDALAIGCGMGHGPRAVSSLKQAIASDLPLLLDADALNLLASETALADALAARAVIDVDGNADAESGGTACACILTPHPLEAARLMGCDAAQVQRDRIGTARALAARFGAVVVLKGAGTVIASPDGRAAVNPTGNAGLATGGTGDVLGGIVGALLAERLPSYEAALAGVYLHGWAADTLTASGRGPAGLTAGELAPVVRDLLNRLLLICGSRRYSGGALLTGLGGRASGVGMLSIAVPESLKSIMVAQLPEALIIGCPETESGAIAQLQLPENTDLSKFNAIACGPGLTRDANPILQQVLESTVPLVLDADGLNILADMGTIPTLQKRQAPTVLTPHAGEFKRLFPDLSDANQDRVKAVREAAAQSHAVVLLKGARTAIANPQGTVWLNPESTPALARGGSGDVLTGLLGGLLAQASSKNIPVEDITATAAWWHAQAGILAAQERTELGVDAHTLTQYL",
    "response#3": "MRAIDRATIDEIGLPGAVLMETAGRAVAEVVRARRRAGATVAVVCGAGNNGGDGYVAARVLREWGYAASAYLAADRAKVAGDAALHLRVLEQCGGRVASIATEADLLEHERAIERADVVVDALFGTGLARDVTGHYARVVAVLNRARGFKVAVDIPSGLAADTGAVLGVAFNADATVTMAFDKPALVSSPGFARCGEVTVAEIGIPAELAERDDAAAALPATTPVDHKGRRGHALVIAGSPGKWGAGRMAARAALRAGAGLVTLAGPRADAAAPDEVMTAAIDPEAAGFDVALAELLAGKRAVAIGPGMPATAAGAALVRAVLERAEVPVVIDADALNHMAGDLAAVAAARAPVVLTPHPGEAGRLLGCSAADIERDRIAAVRRLAERTRAVVVLKGARTLVCDGAVDGFTTVNPTGNAAMATAGAGDVLTGVIVALAAQGAPPADAARAGVFLHGLAGDRAARGRIDAGLMAGDLIDELPRARADLTR"
  },
  {
    "reference": "MKIVQETISTKTKKNIAIIISRYNNFINQHLLDGALDILKRIGQINQKNIPIIHVPGAYEIPIIASIISKQKKYNAIIALGTIIKGHTLHYSHISHAVNSGLTNISITNNIPISIGIITANNIEQAIERAGTKLGNKGSEAALTALEMINIINILAQNK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be designed to catalyze the following reaction (2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-   ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2   H2O + phosphate.2. The protein must exhibit the following characteristics: Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2- butanone 4-phosphate.3. The designed protein should be able to compete with endogenous proteins or inhibitors for (2S)-2-hydroxy-3-oxobutyl phosphate, 5-amino-6-(D-ribitylamino)uracil binding.4. The protein design should consider the endogenous proteins and enzymes present in the riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D- ribitylamino)uracil: step 1/2 pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MRETALLLAAGQSSRMKNNIKKQWLYVGTEPLWLYCVRKFLDSKKIDFIKVVINPNDYSYYQKYCQNYQLIEPIGGKQRQDSVLKGLSVANSDFXFXHDAARPFVSKQLINKLIEAVXTHKAASPAIPVSDALWXEXNYIQGTVNREXVFQXQTPQGFKXXIIXEAXKNXKXGFXDDXAXXEXGXKIXIVEGXEXNXKITFXXDXKKXDKRXNXYYHV",
    "response#2": "MSILSGKKIVVIGLGTSGLSCVDFFLQKGANPVAVDTRETPPEMENLRKKYSGKLQFHLGEFSESWLEDAEMVVLSPGISIKTAEIQKALQKGIPVWGELELFAQARDTPIVAXVXGXKXXGAXAMXTXEXLXXLXGXVXVXNAXSEXSGXXNXYXNXICNXNXXXNWXS",
    "response#3": "MSNRYKFNTIEEAIEDIRQGKMIILVDDEDRENEGDFICAAQFATPEAINFMAKYGRGLICVPITEERCEELDLPLMVQKNTDNHQTAFTVSIDARHGTTTGISAFDRAQTILTAIDPNSRPSDLARPGHIFPLRARKGGVLVRTGQTEGSVDLARLAGLKPAGVICEIMNEDGTMARMPDLMKFAKKHNLKIVTIADLIKYRMQKEMLVKCIA"
  },
  {
    "reference": "MHVLRTPDSRFENLEDYPFVAHYLDVTARDTRPLRMHYLDEGPIDGPPIVLLHGEPTWSYLYRTMITPLTDAGNRVLAPDLIGFGRSDKPSRIEDYSYQRHVDWVVSWFEHLNLSDVTLFVQDWGSLIGLRIAAEQPDRVGRLVVANGFLPTAQRRTPPAFYAWRAFARYSPVLPAGRIVSVGTVRRVSSKVRAGYDAPFPDKTYQAGARAFPQLVPTSPADPAIPANRKAWEALGRWEKPFLAIFGARDPILGHADSPLIKHIPGAAGQPHARINASHFIQEDRGPELAERILSWQQALL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A key design requirement for the protein is the optimization of the Proton donor, Proton acceptor, Nucleophile to achieve high catalytic efficiency for 1-haloalkane + H2O = a halide anion + a primary alcohol +   H(+).2. The protein must be able to haloalkane dehalogenase activity.3. The designed protein must have at least one AB hydrolase-1 domain. The designed protein sequence is ",
    "response#1": "LPRFNPKAFLSMVRGKKMNFIGDSVARNHMESLLCLLSMEETPKDIYKDGEDRNRIWYFPKHDFTLSTSWTKFLVEERERRDSNNTGTGLFDLDIGKIDEGWFNGLPNTDIAIVSAAHWFFRPIFIHRGDETLGCIYCNLPNMTQISPEEGFKLVYSAVLRQINECEMCKKDLVTVLRTISPAHFENGTWDTGGTCSRTSPFGENKIDLQSNEMKIRKSQIEQLEGITKRGNKAKKFAVLDVTRVMQMRPDGHPNGYWGNKWMKGYNDCVHWCLPGPIDAWNDFLMR",
    "response#2": "LPRFNPKAFLSMVRGKKMNFIGDSVARNHMESLLCLLSMEETPKDIYKDGEDRNRIWYFPKHDFTLSTSWTKFLVEERERRDSNNTGTGLFDLDIGKIDEGWFNGLPNTDIAIVSAAHWFFRPIFIHRGDETLGCIYCNLPNMTQISPEEGFKLVYSAVLRQINECEMCKKDLVTVLRTISPAHFENGTWDTGGTCSRTSPFGENKIDLQSNEMKIRKSQIEQLEGITKRGNKAKKFAVLDVTRVMQMRPDGHPNGYWGNKWMKGYNDCVHWCLPGPIDAWNDFLMN",
    "response#3": "LPRFNPKAFLSMVRGKKMNFIGDSVARNHMESLLCLLSMEETPKDIYKDGEDRNRIWYFPKHDFTLSTSWTKFLVEERERRDSNNTGTGLFDLDIGKIDEGWFNGLPNTDIAIVSAAHWFFRPIFIHRGDETLGCIYCNLPNMTQISPEEGFKLVYSAVLRQINECEMCKKDLVTVLRTISPAHFENGTWDTGGTCSRTSPFGENKIDLQSNEMKIRKSQIEQLEGITKRGNKAKKFAVLDVTRVMQMRPDGHPNGYWGNKWMKGYNDCVHWCLPGPIDAWNDFLMG"
  },
  {
    "reference": "MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELLKTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQLFQRLLVWLVANVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDCYLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The glutathione binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must have high specificity towards transferase activity.3. The presence of GST N-terminal domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "DPKKMPIMSVWMEVEGQKFEPHASKLTWELGIKPIIGMTTDDDAVKESEVQLSKVLDIYETRLAESKYLGGDSFTLVDLHHIPNIYYLMSTKVKEVFDSRPRVSAWCADILARPAWVKGLEKLQR",
    "response#2": "KKMPIMSVWMEVEGQKFEPHASKLTWELGIKPIIGMTTDDDAVKESEVQLSKVLDIYETRLAESKYLGGDSFTLVDLHHIPNIYYLMSTKVKEVFDSRPRVSAWCADILARPAWVL",
    "response#3": "CPWAQRTLITRALKGLAPIIGCSVAHWHLDDKGWRFLEEGDGKTNERHWFDIAGGISSVNLNTSTPVANIPNNAHRLLVDGTDEPHYGYKRLSDFYFKTKPDYKGRFTVPVLWDLETCTIVNNESSDIIGII"
  },
  {
    "reference": "MSYRSIYRTLRPVLSSSVQSSGLGIGGFRGHLISHLPNVRLLSSDTSSPVSGNNQPENPIRTADGKVISTYWGIPPTKITKPDGSAWKWNCFQPWDSYKPDVSIDVTKHHKPSNFTDKFAYWTVQTLKIPVQLFFQRKHMCHAMLLETVAAVPGMVGGMLLHLKSLRRFEHSGGWIKALLEEAENERMHLMTFIELSQPKWYERAIVFTVQGVFFNAYFLAYVISPKLAHRITGYLEEEAVNSYTEFLKDIDAGKFENSPAPAIAIDYWRLPKDATLRDVVYVIRADEAHHRDINHYASDIQFKGHELKEAPAPIGYH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must be able to perform ubiquinol:oxygen oxidoreductase activity under specific conditions. The designed protein sequence is ",
    "response#1": "SGNLVTLYIGLELLSITTYVLVGLRKRSSLSAEAAFKYVVTGGIASAFVLFGMSYLYGVTGSVSLADFPTALPQAFTDYKALVYVGFFFLIAGFGIKIAAAPFHIWAADVYQGAPTPVSAFLAVIAKGAALAAVFRFLYSSAFFLTGGPGKQAGDDVFFALLVIAAAAMIAGTVSALRQKQVKRLLALSGVANAGYLLVPIAISVTIIHSNNFSEFVFYLVAYLLMNVGAFAVVTVIARAAGHEELKGFSGLYYRAPWTAAAMLIFILSFSGLPVTAGFFGKLFILLGAASVKAYWLVAIMVVSTVIANNLFQLFIGWEGVGIMSFMLIGWWFGRTDANTAALQAVLYNRIGDIGFMLAMAWLMINNSSWDLQHIFMTNMDTLALMGLVIAATGKSAQFGLHPWLPSAMEGPTPVSALLHSSTMVVAGIFLLIRFHPMIKDNPTILTTTLCLGAITTLFTAICAITQNDIKKIVAFSTSSQLGLMMVTIGLNQPYLAFLHICTHAFFKAMLFLCSGSIIHNLNDEQDIRKMGGLLNIMPITSSALITGSLALMGTPFLAGFYSKDSIIEAMNTSHTNTWALIITLIATALSALNY",
    "response#2": "SGNLVTLYIGLELLSITTYVLVGLRKRSSLSAEAAFKYVVTGGIASAFVLFGMSYLYGVTGSVSLADFPTALPQAFTDYKALVYVGFFFLIAGFGIKIAAAPFHIWAADVYQGAPTPVSAFLAVIAKGAALAAVFRFLYSSAFFLTGGPGKQAGDDVFFALLVIAAAAMIAGTVSALRQKQVKRLLALSGVANAGYLLVPIAISVTIIHSNNFSEFVFYLVAYLLMNVGAFAVVTVIARAAGHEELKGFSGLYYRAPWTAAAMLIFILSFSGLPVTAGFFGKLFILLGAASVKAYWLVAIMVVSTVIG",
    "response#3": "SGNLVTLYIGLELLSITTYVLVGLRKRSSLSAEAAFKYVVTGGIASAFVLFGMSYLYGVTGSVSLADFPTALPQAFTDYKALVYVGFFFLIAGFGIKIAAAPFHIWAADVYQGAPTPVSAFLAVIAKGAALAAVFRFLYSSAFFLTGGPGKQAGDDVFFALLVIAAAAMIAGTVSALRQKQVKRLLALSGVANAGYLLVPIAISVTIIHSNNFSEFVFYLVAYLLMNVGAFAVVTVIARAAGHEELKGFSGLYYRAPWTAAAMLIFILSFSGLPVTAGFFGKLFILLGAASVKAYWLVAIMVVSTVIATDLMTIFLGLELMSISLYVLAGFNRDSIKSNEAGMKYFLLGAFSTGFLLYGMALIYGVTGTTRVAKIASLVTQVGGASNTMLLIGMFLMLTGFLFKIAAAPFHMWTPDVYEGAPTPMTAFMSAGPKAAGFAAMLRLLLVAFPAMIADWSDLLWILAVLTMTVGNFTALRQDNIKRMLAYSSIAHAGYCLVGFASGTATGTSGILFYMLSYTFMNIGAFAVIVLVGKKGEPNGTVMDYAGLGHKRPLLALAMTVFMFSLAGMPPTAGFIGKFYLFSGAVQAGYIWLAIIGVLNW"
  },
  {
    "reference": "MLDLDLRRRQGEFRLDVRLSAGPGVTALYGRSGSGKTSVINMVAGLSRPDEGSISVDGRVLFDSRSGIDLPPEARRLGYVFQEHRLFPHLSVRGNLEFGQKLLPSAERTQSLDKVVELLGIESLLDRRPAKLSGGEKQRVAIGRALLASPRILLMDEPLAALDPARKAEVLPFIAQLARRFSVPILYVSHSMDEVLRLADTLALMDGGKVAASGPLESLMGDPGLRPLTGRYEAGAVIGAVVSSHDSGFGISRLAFDGGTLIVGRSELPVGAKVRLRIHARDVAIAIEPPDRVSIRNVLPAIVVSVAPADSFLVDVILACGPTRFWVQITTLAQAQLNLVPGMRVHALIKALTIARGDVASVD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be optimized for plasma membrane-specific folding and stability, while maintaining ATP binding.2. The protein must exhibit the following characteristics: Part of the ABC transporter complex ModABC involved in molybdenum import; Responsible for energy coupling to the transport system.. The designed protein sequence is ",
    "response#1": "VLQHQTLNIAVQLDATKQIVGVLSNWKKQLGKNYVRLAPELENMAMPGDLNSVNIESLLALKPDVVFVTNYAPSEMIKQISDVNIPVVAISLRTGEVGEKGKLNPTLTDEDKAYNDGLKQGIELIAEVFEKKQQGDELVKAAFANRKLLADRLGDVSADKRVRTYMANPDLGTYGSGKYTGLMMEHAGAYNVAAATIKGFKQVSLENVLEWNPAVILVQDRYPDVVPQILNDQGWANIQALKDKKVFLMPEA",
    "response#2": "VLQHQTLNIAVQLDATKQIVGVLSNWKKQLGKNYVRLAPELENMAMPGDLNSVNIESLLALKPDVVFVTNYAPSEMIKQISDVNIPVVAISLRTGEVGEKGKLNPTLTDEDKAYNDGLKQGIELIAEVFEKKQQGDELVKAAFANRKLLADRLGDVSADKRVRTYMANPDLGTYGSGKYTGLMMEHAGAYNVAAATIKGFKQVSLENVLEWNPAVILVQDRYPDVVPQILNDQGWANIQALKDKKVFLMPEA",
    "response#3": "MTIKIQFKQTLGETNFDIDLSLPRNEISALFGRSGAGKTTLINVISGLVTPQQGRIAIGDHVLFDSEQGINLPTHKRKIGYVFQDSRLFPHYSVQGNLLYGVKEKDDAYFDAVTDLLSIKPLLKRFPISLSGGEKQRVAIARALLSKPDLLLMDEPLASLDMPRKREVMPFLEELSDKVNIPIIYVTHSLQEILRLAQHLAIIDKGQVTTSGKLEEVWASHAMRPWQSFL"
  },
  {
    "reference": "MKRAFERMSMAIAIRNQQKKVKIDLRQIRRRVQKLLRLVDCREKEISLLFVDDEEIQEINQRYLGRNYPTNVISFSLSEGEFSSVNPDVLGDIVISVDTASRDAERGNIPVSDELDFLIIHGLLHILGYNHENNNPEETACMQKKEQELFFLLHGYPLDF",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must have high specificity towards zinc ion binding. The designed protein sequence is ",
    "response#1": "PGELPAREPWDVVQLARHIERPHTLEYVGFVFDEFQELHGDRLFEEDHAIVGGLARLGELPLMVIGHQKGHTTGEMMERNFGMPQPEGYRKSMRLMRYAARFGLPIVTFVDTPGAYPGIGAEERGQSVAIAESIMQMSRLPIPIVTVVTGEGGSGGALALAASDRMLMLENSYYSVISPEGCATILFKDAAAAPKAAAALRMTAPDLLRLRVMDAIVREPEQGAHTDPAAAATNVKAALVQSLAE",
    "response#2": "PGELPAREPWDVVQLARHIERPHTLEYVGFVFDEFQELHGDRLFEEDHAIVGGLARLGELPLMVIGHQKGHTTGEMMERNFGMPQPEGYRKSMRLMRYAARFGLPIVTFVDTPGAYPGIGAEERGQSVAIAESIMQMSRLPIPIVTVVTGEGGSGGALALAASDRMLMLENSYYSVISPEGCATILFKDAAAAPKAAAALRMTAPDLLRLRVMDAIVREPEQGAHTDPAAAATNVKAALVQSLARL",
    "response#3": "PGELPAREPWDVVQLARHIERPHTLEYVGFVFDEFQELHGDRLFEEDHAIVGGLARLGELPLMVIGHQKGHTTGEMMERNFGMPQPEGYRKSMRLMRYAARFGLPIVTFVDTPGAYPGIGAEERGQSVAIAESIMQMSRLPIPIVTVVTGEGGSGGALALAASDRMLMLENSYYSVISPEGCATILFKDAAAAPKAAAALRMTAPDLLRLRVMDAIVREPEQGAHTDPAAAATNVKAALVQSLAELE"
  },
  {
    "reference": "MDPQVVNLLQETEEDVYRRKTARIRRKVSEIEKECEMYAVKIYNVERDIQRYRHEAQLLSKVLEKKGLNKEKEAGET",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytosol, nucleus, Ino80 complex that is critical for chromatin remodeling. The designed protein sequence is ",
    "response#1": "MVGQLAARRAAGVVLEMIREGKIAGRAVLIAGQPGTGKTAIAMGMAQALGPDTPFTAIAGSEIFSLEMSKTEALTQAFRRSIGVRIKEETEIIEGEVVEIQIDRPATGTGSKVGKLTLKTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSPHGIPIDLLDRLLIVSTSPYSR",
    "response#2": "MVGQLAARRAAGVVLEMIREGKIAGRAVLIAGQPGTGKTAIAMGMAQALGPDTPFTAIAGSEIFSLEMSKTEALTQAFRRSIGVRIKEETEIIEGEVVEIQIDRPATGTGSKVGKLTLKTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSPHGIPIDLLDRLLIVSTSPYSR",
    "response#3": "MVGQLAARRAAGVVLEMIREGKIAGRAVLIAGQPGTGKTAIAMGMAQALGPDTPFTAIAGSEIFSLEMSKTEALTQAFRRSIGVRIKEETEIIEGEVVEIQIDRPATGTGSKVGKLTLKTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSPHGIPIDLLDRLLIVSTSPYSR"
  },
  {
    "reference": "MSNILPTQRIEFDLDRNQTLPVSDQFYFPKDSLGDKTYLCGHSLGLINKNAEPYLRKQLNNWKNLAVEGHFKGDSPWIEYLAAMDTQMARIVGAKENEVGIMNSLTANIHFLFSGFYRPAGNKYKVIMEATAFSSDHYAVASHLEVRGVKPKEGIIFWHPNEATGLYEVADLRQLVSKHADELALIFVGGINYLNGQFLDISAITELAHQHDILAGFDLAHAAGNVLLDLHSWNVDFACWCTYKYLNGGPGSPGGIFIHQRHHGRQIYFKGWWGNSMENRFEMLNHFTPEKGVAGWQVSNPTILALPPLKASLEIFDQFGMNQLRNKSILLTSYLVEGLNNLNRENFVINTPVQAEMHGAMICLKLKSKSQDIFDKISTEGIITDFRSPDTIRLAPHPLYNSFEDCFRFLAAFEKALG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. We require a protein that can specifically bind to the metabolites produced in the NAD(+) biosynthesis pathway.2. The protein should be able to bind pyridoxal 5'-phosphate ligand in a variety of conditions3. The ideal protein would be one that plays a critical role in 'de novo' NAD biosynthetic process from tryptophan, anthranilate metabolic process, L-kynurenine catabolic process through pyridoxal phosphate binding within cytoplasm.4. The designed protein have Aminotransferase class V domains with specific sequence motifs that are crucial for its activity.5. For general function, the protein need meet that Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively.6. The protein must be engineered to have a cooperative binding mechanism with pyridoxal 5'-phosphate to ensure proper regulation.7. The desired protein should be able to perform H2O + L-kynurenine = anthranilate + H(+) + L-alanine The designed protein sequence is ",
    "response#1": "MIIRKVKTYLDEELDKWARTGVHGHFNGQRPWALADECILDLMAELVGAQRQELALMNGLTVNLHLQLLSFFKPTPRRYKILLEAKAFPSDHYAVESQLRLHGLDVEQCMLLVQPREGEETLRMEDILAVIEKEGDSIAVILFSGVQYYTGQLFDIPRITKAGHEKGCFVGFDLAHAVGNVELHLHDWGVDFACWCSYKYLNSGAGGLAGAYIHEKHSQTIKPTAGWWGHDHKELKKLMAPIVGASPSEVCPMNTLTVNLHLLMVSFYRPNAKRYKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVKPKEGEYVLQTADIIKEIQENGDEIALVLFGGVNYFTGQLFDMPAITKAGHAVGAVVGFDLAHAAGNVPLSLHDWNVDFACWCSYKYQNAGPGGISGIFVHEKHFGNTEMPRFAPRAMMMMMGIEGVHKELKKLMAPIVGASPSEVCPMNTLTVNLHLLMVSFYRPNAKRYKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVKPKEGEYVLQTADIIKEIQENGDEIALVLFGGVNYFTGQLFDMPAITKAGHAVGAVVGFDLAHAAGNVPLSLHDWNVDFACWCSYKYQNAGPGGISGIFVHEKHFGNTEMPRFADMLPYDKFIDHKELKKLMAPIVGASPSEVCPMNTLTVNLHLLMVSFYRPNAKRYKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVKPKEGEYVLQTADIIKEIQENGDEIALVLFGGVNYFTGQLFDMPAITKAGHAVGAVVGFDLAHAAGNVPLSLHDWNVDFACWCSYKYQNAGPGGISGIFVHEKHFGNTEMPRFA",
    "response#2": "MHKELKKLMAPIVGASPSEVCPMNTLTVNLHLLMVSFYRPNAKRYKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVKPKEGEYVLQTADIIKEIQENGDEIALVLFGGVNYFTGQLFDMPAITKAGHAVGAVVGFDLAHAAGNVPLSLHDWNVDFACWCSYKYQNAGPGGISGIFVHEKHFGNTEMPRFAHKELKKLMAPIVGASPSEVCPMNTLTVNLHLLMVSFYRPNAKRYKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVKPKEGEYVLQTADIIKEIQENGDEIALVLFGGVNYFTGQLFDMPAITKAGHAVGAVVGFDLAHAAGNVPLSLHDWNVDFACWCSYKYQNAGPGGISGIFVHEKHFGNTEMPRFAHKELKKLMAPIVGASPSEVCPMNTLTVNLHLLMVSFYRPNAKRYKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVKPKEGEYVLQTADIIKEIQENGDEIALVLFGGVNYFTGQLFDMPAITKAGHAVGAVVGFDLAHAAGNVPLSLHDWNVDFACWCSYKYQNAGPGGISGIFVHEKHFGNTEMPRFAL",
    "response#3": "MIRKVKTYLDEELDKWARTGVHGHFNGQRPWALADECILDLMAELVGAQRQELALMNGLTVNLHLQLLSFFKPTPRRYKILLEAKAFPSDHYAVESQLRLHGLDVEQCMLLVQPREGEETLRMEDILAVIEKEGDSIAVILFSGVQYYTGQLFDIPRITKAGHEKGCFVGFDLAHAVGNVELHLHDWGVDFACWCSYKYLNSGAGGLAGAYIHEKHSQTIKPTAL"
  },
  {
    "reference": "ADDEDCCSYEDRREIRHIWDDVWSSSFTDRRVAIVRAVFDDLFKHYPTSKALFERVKIDEPESGEFKSHLVRVANGLDLLINLLDDTLVLQSHLGHLADQHIQRKGVTKEYFRGIGEAFARVLPQVLSCFNVDAWNRCFHRLVARIAKDLP",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must have high specificity towards oxygen carrier activity, oxygen binding. The designed protein sequence is ",
    "response#1": "RFFSLILEIAPVAKNMFSFLKDSDELPENNPKLRAHAVKVFKMTCESAIQLREKGEVVVGETTLKYLGSIHLQKRVADPHFEVVKEALLRTVKEATGNKWKDEMKEAWSEAYDQLASAL",
    "response#2": "WQDDLNIGIDVIDQQHRRIIEMLNHLHVAQASMQRAAVGEVIDEVVDYTMSHFAFEEELMEEAGYPFCAAHKRVHEVFIKRVSEYRMRFQAGEDISDELRTMLSRWLFNHIRGDDQAYAEA",
    "response#3": "WQDDLNIGIDVIDQQHRRIIEMLNHLHVAQASMQRAAVGEVIDEVVDYTMSHFAFEEELMEEAGYPFCAAHKRVHEVFIKRVSEYRMRFQAGEDISDELRTMLSRWLFNHIRGDDQAYAEE"
  },
  {
    "reference": "MKLATIVLAAGEGTRMKSDLPKVLHTLAGLPMLVHVLSVCKELKPERQIVVLGHQHEQVLAAIGADVDYVHQKEQNGTGHAVLVTEERMKDFDGAVLVLAGDIPLLQAETLKNLIDVFIEDKPAAALVTAELENPFGYGRIIRDRAGTVARIVEQNDATSEERGVKEINAGIYCFDSRRLFSALGLIGNHNKKSEYYLTDVISVLRNQGEVVLASGASPDEVLGVNTRSELADAESIIQARLRRIFMEAGVTFINPETSYLEATVDIERDTSILPNCFIQGTTKIGKGSKIGPNARIIDSSIGENANVQYSVINSANVEDEVDIGPFCYIRPGTRLMRGSKAGTFVEIKKSEIGPGSKVPHLSYMGDAVIGEDVNVGAGSITCNYDGVEKHETIIEDGAFLGSDTMLIAPVKIGRGAVTGAGSSITEDVPPDSLSVERAEQKTVPDWAKQRRSKT",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have a high specificity for acetyl-CoA binding, with minimal off-target effects.2. The designed protein should interact with enzymes and substrates within the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MAAGLGTRMKSRRAKVLHLLAGRPLISYVYRTAMALEPSSILIIVGHQAEEVEAIVNRDLEIIAASGRSAPSVKFILQAEQRGTGHAVMQAAPELRGKEGTLMVLSGDVPLVRVEDLLSLISFHKKNGFSATAMTTMLDDPRGYGRILRDSQGQFLRIVEQRDATEEQNRIKEINAGIYCFEIEHLLPALDKLTTANAQGEYYLTDVLEIMKKNGGRIGLYTPDEDTGGVTLADPARFDLRGRLEAEPDVSIDVNVVIEGEVRLASGVRIGPNCLLRDCVVGPGTEIQANCVIEEAEVGADARIGPFARLRPEARLADRTHVGNFVEIKKTRVGLGSKVNHLTYLGDAEVGAGVNVGAGTITCNYDGANKFKTRIGDGAFIGSNAALVAPVTVGAGATIGAGSVVNKDAPPDQLTVARARQVNKKGLSRKL",
    "response#2": "MAAGLGTRMKSRRAKVLHLLAGRPLISYVYRTAMALEPSSILIIVGHQAEEVEAIVNRDLEIIAASGRSAPSVKFILQAEQRGTGHAVMQAAPELRGKEGTLMVLSGDVPLVRVEDLLSLISFHKKNGFSATAMTTMLDDPRGYGRILRDSQGQFLRIVEQRDATEEQNRIKEINAGIYCFEIEHLLPALDKLTTANAQGEYYLTDVLEIMKKGGLRVLGYQVEDPLEIMGVNSREQLAVAEKVLQRRALEDLMLAGVRLADPARFDVRGTLQCGQDVEIDVNCIFEGQVSLGDGVRIGANCVIRNASIAAGARIHEFTHIDGEAAGASVGEGALIGPFARLRPGAQLGAEVHIGNFVEVKNSTLARGAKANHLAYLGDATVGERVNYGAGSITANYDGANKHRTEIGNDVHVGSNCVLVAPVKLGAGATIGGGSTITKDVAEGELSVARGKGVTIIDPDSTYISPEVEIGADTVIYPQVIIEGRSQIGNDCTIHSWSRLTNVRLGNGVTVHNSCVISDSILHQHSTVGPFAHLRMGAELCEGARVGNFVEVKKSRLGRDSKSMHLTYLGDATIGERVNIGAGTITCNYDGKQKHQTFIEDEVRIGSDTMLVAPVRVGRRSVTGAGTVVIKDVPDNSLTLGYGVTIIDPNNTYIDVDVKIGMDTIVYPGTIIEGITEIGEDCIIGPSSRIVKSKISKGVEVKNSVILESSVNDNTSVGPFAYIRPGSVIGKQVKIGDFVEVKKSIIGDKTKLSHLTYVGDAEVGSNVNLGCGVVVVNYDGKKKNKTKIGDNSFVGCNVNLISPVEVKANAYIAAGSTITDEVPENSLAIARAR",
    "response#3": "MAAGLGTRMKSRRAKVLHLLAGRPLISYVYRTAMALEPSSILIIVGHQAEEVEAIVNRDLEIIAASGRSAPSVKFILQAEQRGTGHAVMQAAPELRGKEGTLMVLSGDVPLVRVEDLLSLISFHKKNGFSATAMTTMLDDPRGYGRILRDSQGQFLRIVEQRDATEEQNRIKEINAGIYCFEIEHLLPALDKLTTANAQGEYYLTDVLEIMKKGDKNAAGEYYLTDIIKIIKNDGKVVEYKMPDREETLGINTRVQLAQAEKVMRERINRKWMLAGVTLADPARFDLRGRLEAEPDVSIDVNVVIEGEVRLASGVRIGPNCLLRDCVVGPGTEIQANCVIEEAEVGADARIGPFARLRPEARLADRTHVGNFVEIKKTRVGLGSKVNHLTYLGDAEVGAGVNVGAGTITCNYDGANKFKTRIGDGAFIGSNAALVAPVTVGAGATIGAGSVVNKDAPPDQLTVARARQRNIEDWVRKRKQG"
  },
  {
    "reference": "MENKGNNNNNGGNNNNSGNNKNGMTIMIFILAALLVLFLTSLLNSCAKDATNKEITYSEFIDMVEKDKVESVTFTSSRINITPKGENKLYRINYYTAELNDESLLPLLKEHGVKFGGTVEDVSSVIMWNMLSYILPLVLVWVLLYFLIFRKMGSGGMMGVGKTTAKVYVEKSTGVTFKDVAGQDEAKESLQEVVDFLHNPRKYTDIGAKLPKGALLVGPPGTGKTLLAKAVAGEAKVPFFSLAGSDFVEMFVGVGASRVRDLFKEAQKQAPCIIFIDEIDAIGKSRDTRYGGNDEREQTLNQLLAEMDGFDTSKGLLILAATNRPEVLDKALLRPGRFDRRIIVDKPDLKGRVETLKVHAKNVLLDDSVDLDAIALATSGAVGSDLANMINEAAINAVKQGRKYVNQSDLFESVEVVIAGKEKKDRIMGPKEKKMVAYHEVGHALVTALQKDAEPVQKITIVPRTMGALGYTMQVPEEEKFLMTKNELVAHLVTYMGGRAAEEIVFDSVTTGASNDIEQATKIARAMVTQYGMSEKFGLMGLVTIENQYLDGRASLNCGEETAAQIDQEVMKILKESYDEATRLLLENREILDKISQHLYEHETITGKEFMKIFRELKGIPEPEEKTEAEKAAEGFQAESAKSWEVKEEEILVNTLKEEDLPPEITGAKEDLSPENTRAKEEDLPKEQSLEVNTLEGEELPGELTALQREDRSSGDEAKSETEVELIPVMVNMPKVQETVSEEEAKPADVETKEELKPADVETKNEAK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The designed protein should be involved in protein catabolic process, proteolysis by localizing to plasma membrane and exhibiting ATP hydrolysis activity.3. The designed protein should have a high specificity for ATP, Zn(2+) binding, with minimal off-target effects.4. The designed protein must have at least one AAA+ ATPase domain.5. For general function, the protein need meet that Plays a role in the quality control of integral membrane proteins.; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins The designed protein sequence is ",
    "response#1": "IPKGVLLYGPPGTGKTLLARAVAGEAGVPFYTISGSDFVEMFVGVGASRVRDLFEQAKQNAPCIIFVDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFDARGGIILIAATNRPDILDPALLRPGRFDRQIPVSAPTLEERLEIVKRQLEVDKLNFDVDPAILARGTPGMAGADLANLVNEAALFAARGNKRLVDMDDFTRAIDRLIPSGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFDQAKKNAPSIIFIDEIDAVGRRRGNGMGGGHDEREQTLNQLLVEMDGFQGDEGVIVMAATNRSDVLDPALLRPGRFDRKILVGRPDRETLETILNRRTELPLDKNVSIEAIARRTPGFAGADLANLLNEAAILTARRRKPAISLEEIEFGVQYLMDLDSKKKRLIAYHEVGHALLMTLLKHSDPLNKVTIVPRSGGIGGFAQQVFNEEMVDSGLYTRAWILDRIVMTLGGRAAEQEVFGDSEITVGASNDLKVVSTLAREMVTRYGMSDLGTLALESQSGEVFLGRGWMSQPEYSEEVAAKIDRAVREIAVNCYEKACQILREHRELVDRLVDQLLDEEMIEGDEFRKLVEQY",
    "response#2": "VLLYGPPGTGKTLLARAVAGEAGVPFYTISGSDFVEMFVGVGASRVRDLFEQAKQNAPCIIFVDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFDARGGIILIAATNRPDILDPALLRPGRFDRQIPLNFMDASTLIGRKIPSGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFDQAKKNAPSIIFIDEIDAVGRRRGNGMGGGHDEREQTLNQLLVEMDGFQGDEGVIVMAATNRSDVLDPALLRPGRFDRKILVGRPDYAGRLAILDVHAREMGRTLDVDPAILARGTPGMAGADLANLVNEAALFAARGNKRLVDMDDFD",
    "response#3": "VLLLGSSGVGKTMLARAVAGEANVPFFSISGSEFVEMFVGVGSARVRDLFSTAKKASPSIIFIDELDAIGRHRGAGIGGGHDEREQTLNMILVEMDGFQKDTGVLVFAATNRGDILDPALLRPGRFDRKIIVMHNLKDETGLGRIEKKARELNI"
  },
  {
    "reference": "MTFEQMLQGAEVLSLSGNADVTGLAYDSRQVKPGYVFLAMRGETSDGNRFIDRAIAAGAAAIVTDSAVEQPRPGISWARVAHGRRALGRLSANFYKRPAERLAITGITGTNGKSTTAFLVESILSAAKRKSVLVGTIEYHVAGKILPAPHTTPESLELNQFLAEGLGQGATEAVMEVSSHALAQQRVFAVPFDVAVFTNLTRDHLDYHHSMDSYFDAKRMLFEGCGTDTPRSAVINTDDEYGQKLVQISKKSGSRVSTYGWTNGDFRAKSVEITARGTRFDMITPNGAIALFSPLIGKVNVYNILAAAGAAMARGCSHEDIANGVASLIRVPGRFERVDCGQPFTVVVDYAHTDDALRNLTALAREFVTQANHKRRVLTVFGCGGDRDRAKRPLMGEAAGKGSDFVVLTSDNPRSEDPLAIMNDAVVGIQRSGTKYAMEPDRRAAIALAVREAAPGDIVLIAGKGHEKVQVSREGPVPFDDVNVAREVLATAGYECQAATAGAGQQG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.2. The desired protein should be able to perform ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate3. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.4. A protein that is capable of catalyzing reactions within the Cell wall biogenesis, peptidoglycan biosynthesis pathway with high efficiency and specificity.5. The protein contains novel Mur ligase N-terminal catalytic, Mur ligase C-terminal, Mur ligase central domains that confer a unique function or activity.6. The meso-2,6-diaminoheptanedioate, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MEQGMTLDSRQVGNGDLFVALPGHVVDGRDYIDAAVAKGAVAVLQEHAHAGVLSQDPVLTIGLPNLAHKLSQIASAWYGHPSQSLRVVGVTGTNGKTTTTFLVEAMLQAASRKSILIGTIEVHLAGEVRESPHTTPEARDLLALFAEGRQAGASEAVMEMSSHALDQERVWGMPVDVAIFTNLTQDHLDYHGTMEKYFAAKQRLFAGVGTPPPRVAVINAEDEYGHRLEKFALREGTVVATYGMTKGEWRAEEVILRAGETRFVLKSPAGEILLRTSLTGRVNVLNTLAASAACALAEGLELDAIAAGIAALPGVPGRMELFQVANKPKIVVDYAHTPDALEKALQALRVHCEGKLWVIVGCGGDRDKGKRPMMASIAEQLADYVVLSDDNPRGEMPSAIVDDMLAGMNNPAAAMIEHSRFEAAKIALAQAGTSDIILLAGKGHETYQELAGQREPFSDRDVARQAIAARGGRA",
    "response#2": "MKLNTIIENVNYSLIKGSIDTEEITGVEYDSRRVREGSLFVAMRGGTTDGNKFIRQAMDKGAGGVLTDSRKIPSNISLISEITGVEYDSRRVREGSLFVAMRGGTTDGNKFIRQAMDKGAGGVLTDSRKSAYIISNAFYENPSTKLKLVGITGTNGKTTTAYLTYQFLNKLGNKTAYLGTIGFKLPDEEIGTENTTPDILSIYTLLLHAIEKECKTVVMEISSHALSYERIYGLHIDIAAFTNLTEDHLDYHKTMENYLNAKLKIIDYLPENGKLIVNTDDEYGQKFIDKFGKGITIGYNKTCNYFIKKADITPAETKITFVHDDTTYNVTTNLTSKFNVYNYITSLATGIALNLNIDSIKNSLINIKTVPGRFELVQGGQDFTVIVDYAHTPDSLENVLKTVREFAHKKIFVIVGCGGDRDKTKRPIMAKIALEWATDPIFTSDNPRSENPAQILRDMEEGVHEKNYQVIEDRKEAIEYAISAATSGDVILIAGKGHENYQEIKGVKYHFDDKEILKKIF",
    "response#3": "MLLKELLKNVKLHSILGNVDIDINSRKVKPGQIFVAIKGHTVDGHDYISDAINHGAIKIIAEKNVSSSVPVENYYFAVTGTNGKTSVSWMLQKILNDNLGHKAGLIGTIQNILGEEILPAKNTTPESYVVQEYFAKMVNAGCQYCVMEVSSHALFLDRVYGINFEIGIFTNLSQDHLDFHKDFDSYFEAKKKLFDNSKCSIINIDDEYGRRLFEDLTDKSITYGIDNKADYRASNIKLKSNGMEFDLIYKNLVVPIKINLIGKFNVYNALAAISTCLLEGIPLNDIKEGLENVKGVSGRMDCIMNPGKPLVVVDYAHTPDALEKALATLQEIKPQGASLWCVFGCGGNRDKGKRPLMGAAAVAGADKVVVTSDNPRMEEPQAIIDDILPAVPNPEHIEADRRAAIEYAVKHAAADDIILIAGKGHETYQIFKDKTIHFDDREVVKQILKEK"
  },
  {
    "reference": "MAFLALGINHKTASVDVRERVAFTPDQMVEALRQLCRVTPTREAAILSTCNRSELYLQQDEVEAEAVLAWLASYHRLSLDELKACAYVHVDDQAVRHMMRVASGLDSLVLGEPQILGQMKSAYAVAREAGSVGPLLGRLFQATFSTAKTVRTDTAIGENPVSVAFAAVSLARQIFSNLQRSQALLIGAGETITLVARHLHEQGVKRIVVANRTLERASALAAELGAHAILLADIPDELHNSDIVISSTASQLPILGKGAVEQALKRRKHKPMFMVDIAVPRDIESQVGELDDVYLYTVDDLHEVVAENLKSRQGAAQAAEELVAAGTEDFMQRLRELAAVDVLKAYRQHAERIRDDELSKAQRLLANGASAEDALAQLARGLTNKLLHAPSVQLKKLSAEGRVEALSMAQELFALHEGTEKP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design must be focused on enhancing the efficiency of protoporphyrinogen IX biosynthetic process.2. The designed protein should be able to compete with endogenous proteins or inhibitors for NADP(+) binding.3. For general function, the protein need meet that Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).4. Ideal protein should have a binding affinity for key enzymes in the protoporphyrin-IX biosynthesis, 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2 pathway to enhance the overall efficiency of the pathway.5. The protein should be designed to have high stability and activity towards (S)-4-amino-5-oxopentanoate + NADP(+) + tRNA(Glu) = H(+) + L-   glutamyl-tRNA(Glu) + NADPH. The designed protein sequence is ",
    "response#1": "MAHHIFADMSRNKALLIGAGKTIELVARHLAEAGVRSFLVANRTLEKAQALAEAHGGKGILLSEIPDHLSDVDIVISSTASPLPILGKGAVERALKKRKHRPYFMVDIAVPRDIEPEVASLADVYLYTVDDLRQLARQIFDDFTKLRFLLVGAGETIELVARHLINHGAKNIMIANRTHIRAEMLAVKLDIPMQILSLSALQIGLNQADVVICSTGSPDILITKEMVEQAQKQRRFDPMLLIDIAVPRDIDEKAGELDSIYAYSVDDLQNLLADEQRTEAERIVEGELKLLLRSLKHMEVEPLLAEVRGNMESLRRREAERALNKIMNSSDPEGVIEALSRSIVDKIFHDIAISIRQAAERGDEEFLSMCAKLFEAENLISSGVQDFLDQLRALDAVSTLKVFRQRAELLRDVETEKALRALRNGTDPETALRSLARGLTNKLLHQPSVQVRKAMAEGRTEVTECLRELYQG",
    "response#2": "MAHHIFADMSRNKALLIGAGKTIELVARHLAEAGVRSFLVANRTLEKAQALAEAHGGKGILLSEIPDHLSDVDIVISSTASPLPILGKGAVERALKKRKHRPYFMVDIAVPRDIEPEVASLADVYLYTVDDLRQLARQIFDDFTKLRFLLVGAGETIELVARHLINHGAKNIMIANRTHIRAEMLAVKLDIPMQILSLSALQIGLNQADVVICSTGSPDILITKEMVEQAQKQRRFDPMLLIDIAVPRDIDEKAGELDSIYAYSVDDLQNLAAHMLVEAERIVEGELKLLLRSLKHMEVEPLLAEVRGNMESLRRREAERALNKIMNSSDPEGVIEALSRSIVDKIFHDIAISIRQAAERGDEEFLSMCAKLFQAEVIIDAGVQNFMHWLGQRRTVPLIQQLNAQTDEWRAAEIARAKKLIAKGEPMDAVLDALTRGLTQKMLHGALAELHAGDAASREATAHTVSRLLF",
    "response#3": "MAHHIFADMSRNKALLIGAGKTIELVARHLAEAGVRSFLVANRTLEKAQALAEAHGGKGILLSEIPDHLSDVDIVISSTASPLPILGKGAVERALKKRKHRPYFMVDIAVPRDIEPEVASLADVYLYTVDDLRQLARQIFDDFTKLRFLLVGAGETIELVARHLINHGAKNIMIANRTHIRAEMLAVKLDIPMQILSLSALQIGLNQADVVICSTGSPDILITKEMVEQAQKQRRFDPMLLIDIAVPRDIDEKAGELDSIYAYSVDDLQNLAGQLFENLGDIRVLFVGAGEMIDLAATHFAAKTPKSMAIANRTLERGEKLASRFGAEVMRLADLPSRLHEFDAVISCTASTLPIIGLGAVERAVKLRKHRPMFMVDLAVPRDIEPEVKDLPDIYLYTVDDLAHKLLKGNNREAERIVEGELKLLLRSLKHMEVEPLLAEVRGNMESLRRREAERALNKIMNSSDPEGVIEALSRSIVDKIFHDIAISIRQAAERGDEEFLSMCAKLFEAENLISSGVQDFLDQLRALDAVSTLKVFRQRAELLRDVETEKALRALRNGTDPETALRSLARGLTNKLLHQPSVQVRKAMAEGRTEVTECLRELYQL"
  },
  {
    "reference": "MTTSELPEHLAKHEVRYADHRQVERDRLTPMMQHYAEVKDQHLQQILLYRMGDFFECFFQDAIVVARELELVLTSKEAGKEVGRVPMAGIPYHALDRYASQLVEKGYAIAICDQVETAAQAQGPLVRREITRIITPGTILEEGMLQARRNNFLAAVVIAGEHWGLAYADSSTGDYWTSQSTGLEGLTQELYRLQPSEVLFPSAAPDLAGLLRPGQSKPQQIPDCLPDSFCYALRSPMPFELHEARQRLLEHFQLRSLEGCGCEQLPLAIRAAGGLLDYLGETQRESLAPLQKPRTYSLSEFLILDQQTRRNLEITQTQRDGSFHGSLLWALDRTMTSMGGRLLRRWLLQPLLNPEAIRNRQAAIQELCQDGRLRQDLRSLLQKIYDLERLSGRAGAGTANARDLLALAESLLRLPELAQLLSRAQSPLLAQLQQVPPELEQLGDRLQQHLVESPPLQLTEGGLIRSGVAIALDELRQQVESDRQWIASLEASERTATGINSLKVGYSKTFGYFISLSRSKADQVPDHYIRRQTLTNEERFITPDLKERESRILNAQTDLNQLEYDLFVGLRSEVSHHVETIRAIATAVAAADVLAALAEVAVYQNYCCPEIRDDRQLAIQDGRHPVVEQALPSGFYVPNSCGLGSDRGPDLIVLTGPNASGKSCYLRQVGLIQLLAQIGSFVPAKNAQVGICDRIFTRVGAVDDLATGQSTFMVEMDETANILNHATARSLVLLDEIGRGTATFDGLSIAWAVAEYLAREIQARTIFA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must have high specificity towards mismatched DNA binding, ATP binding, ATP-dependent DNA damage sensor activity.2. The protein must exhibit the following characteristics: It is possible that it carries out the mismatch recognition step; This protein has a weak ATPase activity..3. The ATP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MITGPNMAGKSTYMRQVALITLMAQAGSFVPAASARIGIADRIFTRVGAGDNLSRGQSTFMLEMMEAAGILRNATPKSLIVMDEIGRGTSTFDGVSIAWAVAEYIHDTPTCRSRTLFATHYHELTELAATRERIRNFTVAVREWNDQVIFR",
    "response#2": "MITGPNMAGKSSYLRQTGLIVLLAQAGCFVPAEQAEIGVVDRIFTRVGASDNLASGESTFLVEMNEAADILNNATAKSLLLLDEIGRGTSTYDGLAIAWSMCEYIHRQIGARTLFATHYHELAELEGLLPGVVNYNASVLESGDRR",
    "response#3": "EFDDSLRIIRKKLDRLRTDMDNEFAEAAEDLGQEREKKIFLENHKVHGWCMRLTRTEAGCIRNNSRYLECSTQKNGVYFTTKTLQALRREFDQLSV"
  },
  {
    "reference": "MRVMGTQINYQNLWRWGTMILGMIIICSAVDNLWVTVYYGVPVWKDADTTLFCASDAKAYDTEKHNVWATHACVPTDPNPQEMHLENVTENFNMWKNNMVEQMHTDIISLWDQGLKPCVHLTPLCVTLNCHEISNGTNNSSMDKDMIGEIKNCSYNTTTELRDKKQKAYSLFYRLDIVQINNSSRIGSSHNSSHNISQYRLINCNTSAITQACPKVSFKPIPIHYCTPAGFAILKCRDPKFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVIIRSENITDNTKTIIVQLNEPVKINCTRPNNNTRKGVHIGPGQAFFATGDIIGNIRKAWCEVNETQWKKTLKKVAIQLGEHFKNKTIKFDSSSGGDVEITTHSFNCQGEFFYCNTTNLFNTTWSNGSLVNITESNNATNNATILLPCRIKQIINMWQRVGQAMYAPPIPGVIQCTSNITGLLLTRDGGNITNNTEIFRPGGGDMRDNWRSELYKYKVVRIEPLGVAPTGAKRRVVEREKRAIAGIGAVLFGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQSNLLQAIEAQQHLLKLTVWGIKQLRARVLAVERYLRDQQLLGIWGCSRKLICTTTVPWNDSWSNKSQKEIWENMTWQQWDKEISNYTQIIYNLIEESQNQQEKNEQELLALDKWASLWSWFDISNWLWYIKIFIIIVGGLIGLRIVFAVLSVINRVRQGYSPLSFQTLLPASREPDRPEGIEEEGGEQGRGRSIRLVNGFSALIWDDL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should contain Cytoplasmic topological domains.2. For general function, the protein need meet that The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Transmembrane protein gp41: Acts as a class I viral fusion protein; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane3. The designed protein must have at least one Retroviral envelope protein GP41-like, Human immunodeficiency virus 1 envelope glycoprotein Gp120 domain. The designed protein sequence is ",
    "response#1": "MRVRGIQRNWPQWWIWGILGFWMLMIYNVMGNLWVTVYYGVPVWXDADTTLFCASDAKAYDKEVHNVWATHACVPTDPNPQEVNLTNVTENFNMWKNNMVEQMQEDIISLWDQSLKPCVKLTPLCVTLNCTKVNSATNELRNCSYNITTEVRDKQRKVYSLFYKVDIVGGGDNETQYRLINCNTSAMTQACPKITFEPIPIHYCAPAGFAILKCKDKKFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENISNNXKTIIVQLVNPVNITCIRPNNNTRKSIRIGPGQTFYAQGDVIGDIRKAHCNVSKTKWNNTLQEVVKALRKYWGNKTIIFNSSSGGDXELTTHSFNCGGEFFYCNTSGLFNSTWNNTDSXQGNNSNTNGNITLLCRIKQIVNMWQRAGQAMYAPPIPGIIKCESNITGILLTRDGGPXNNSTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTXAKRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLXDQQLLGIWGCSGKLICATNVPWNSSWSNKSQSEIWDNMTWMQWDKEVINYTDIIYDLIEKSQNQQEKNEQDLLALDKWASLWNWFDISNWLWYIKIFIIIVGGLIGLRIVFAVLSIINRVRQGYSPLSLQTLIPNPRGPDRPGGIEEE",
    "response#2": "MRVMGIRKNYQHLWRWGIMLLGMLMICSATEKLWVTVYYGVPVWRDAETTLFCASDAKAYDTEVHNVWATHACVPTNPDPQEIALINVTEYFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLNCTNYKNKTQTVTNSTSSTSGIEMMKNCSFNITTDLRDKVKKEHALFYKLDVVQIDNDSYRLISCNTSVVTQACPKISFEPIPIHYCAPAGFAILKCNDKKFNGTGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVVIRSVNFTDNTKTIIVQLKEPVEINCTRPNNNTRKGIHIGPGRAFYATGDIIGDIRQAYCNISRTKWNNTLEQIVSKLRKQFRNKTIVFNQSSGGDPEIVMHSFNCGGEFFYCNTTKLFNSTWNNTEESNNTKGDIVTLPCRIKQIINMWQEVGKAMYAPPIAGQIRCSSNITGLLLTRDGGNQNNVTEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAIGLGAAFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQXNLLRAIEAQQHMLRLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTSVPWNSSWSNKTQKEIWENMTWLQWDKEISNYTDIIYSLIEESQNQQEKNEQDLLALDKWSTLWSWFDISNWLWYIXIFIMIVGGLIGLRIVFAVLTIINRVRQGYSPLSFQTLTHH",
    "response#3": "MTVMGIRKNCQHWWKWGIMLLGMLMICKAAEKLWVTVYYGVPVWKEATTTLFCASDAKAYSKEVHNVWATYACVPTDPSPQEIPLENVTENFNMGKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTEVKTNDTSTGEIKNCSFNITTSIRDRVTKEHALFYSLDIVSLDNTSYTLISCNTSTITQACPKVSFEPIPIHYCAPAGFAILKCNNKTFNGSGPCTNVSTVQCTHGIRPVVTTQLLLNGSLAEEEVVIRSKNFSNNANTIIVQLNESVVINCTRPNNNTRRGIHIGPGGAFYTTGDIIGDIRKAHCSISREKWNNTLKQVVKKLKEQFNTTTIIFKPSSGGDPEIVMHSFNCGGEFFYCNTTSLFNSTWDDSTNSTGEGENITLPEDITLPCRIKQFINMWQKVGKAMYAPPIRGQIKCSSNITGLLLTRDGSGGNSTTTNETFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRAVGVIGAMFLGFLGTAGSTMGAASITLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTSVPWNTSWSNKSFNYIWDNMTWMQWDKEINNYTGLIYKLLEESQNQQDKNEQELLELDKWANLWNWFDITNWLWYIRIFIMIVGGLVGLRIVFAVFSIVNRVRQGYSPLSFQTRLPAQRGPDRPEGIEEEGGERDRDRSVRL"
  },
  {
    "reference": "MAAAATPDKHFPVSWDMFHRDCRALTWRLAGQGPFDALVTVTRGGLVPAAIVARELGIRVIETICVASYGGASGVPDTQQGSLKILKGVTDAILKIGNGSGGRVLIVDDLVDTGKTARVVRDLLPNAHFATVYAKPMGRPLVDTFITEVSQDTWIFFPWDTGLAFQPPIAGGA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Purine salvage pathway enzyme that catalyzes the transfer of the ribosyl-5-phosphate group from 5-phospho-alpha-D-ribose 1- diphosphate (PRPP) to the N9 position of the 6-oxopurines guanine and xanthine to form the corresponding ribonucleotides GMP (guanosine 5'- monophosphate) and XMP (xanthosine 5'-monophosphate), with the release of PPi; To a lesser extent, also acts on hypoxanthine.2. A protein that contributes to purine ribonucleoside salvage, XMP salvage, GMP salvage by functioning in plasma membrane with guanine phosphoribosyltransferase activity, xanthine phosphoribosyltransferase activity, magnesium ion binding.3. The protein should be designed to catalyze the following reaction diphosphate + XMP = 5-phospho-alpha-D-ribose 1-diphosphate +   xanthine.4. The designed protein should contain one or more Phosphoribosyltransferase domains that are essential for its function.5. A protein should be able to manipulate the activity of enzymes in the XMP biosynthesis via salvage pathway, Purine metabolism pathway, either by activating or inhibiting their function.6. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "MSWDQFHRDTRALTWRLHEAGPFEAIVCITRGGLVPAAIVARELNVRIIETVCIASYHDYKNQGDIKVMKGVGPEITSMRGQGKGVLIVDDLVDTGKTARVVREILPAAHFATVYAKPMGRPLVDTFITEVSQDTWIYFPWDTGLEVPDAVDRDELAWEELHRTSKALAWRLLELGPFEGIVAVTRGGLVPAAIVARELEIRLIETACISSYHGSDQGELAVLKDAPSAGDGTGWLIVDDLVDTGETARALRTMMPKAHFATVYAKPAGRPLVDTFVTEVSQDTWIFFPWDMEPD",
    "response#2": "MKISWDQFHRDTRALTWRLHEAGPFEAIVCITRGGLVPAAIVARELNVRIIETVCIASYHDYKNQGDIKVMKGVGPEITSMRGQGKGVLIVDDLVDTGKTARVVREILPAAHFATVYAKPMGRPLVDTFITEVSQDTWIYFPWDTGLPPIGIPDNL",
    "response#3": "MKIWDQLHRDAKALSWRLMSLGPWAGIVAITRGGLIPAAIIARELDCRLVESVSVVTYDEEKRGDPVVTKYPTAAGDGRGFLLVDDLVDTGTTARVIRKLLPHAHFATIYAKPAGKDIVDTFITEVSQDTWILFPWDTEPLYDEV"
  },
  {
    "reference": "MDIGTRRIFNEDHDIFRRSARRFFQEEVIPYHAEWEKAGEVSREVWEKAGEQGLLAVLTPAEHGGVGADVLSAAIVWEEQMYSNCTGPGFSLHSDIIMPYISNYGSEQQIQRFVPDMAAGRCIGAIGMTEPGRQQSDLQGVKTYAKRDGSDWILNGNKVFISNGWMSDLVVVVAVTDREAKSAAHGISLFLVESGTKGFQKGRKLEKIGLKAQDTAELFFEDVRLPADALLGEPNKGFYYLMNELPQERLVIAGMAVASCEFMFEETRNYVMQRKAFGKTIADLQTVQHTLAELKTEICMGRVFFDNCIQLHAEKRLDAATASMAKYWTSDLQNKVATQCLQLHGGWGYMWEYPIAKAFVDSRIQPIYGGTNEIMKELIGRCIVRRKKQP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a low Km value for eicosanoyl-CoA + H(+) + oxidized [electron-transfer   flavoprotein] = (2E)-eicosenoyl-CoA + reduced [electron-transfer   flavoprotein].2. The protein must be engineered to have a cooperative binding mechanism with FAD to ensure proper regulation.3. The designed protein should have long-chain-acyl-CoA dehydrogenase activity, flavin adenine dinucleotide binding within mitochondrial matrix to regulate long-chain fatty acid catabolic process.4. We require a protein that can specifically bind to the metabolites produced in the mitochondrial fatty acid beta-oxidation, Lipid metabolism pathway.5. The designed protein must have at least one Acyl-CoA dehydrogenase/oxidase C-terminal, Acyl-CoA oxidase/dehydrogenase middle, Acyl-CoA dehydrogenase/oxidase N-terminal domain. The designed protein sequence is ",
    "response#1": "ALAMTEPGAGSDLQGVKTYAKRDGSDWILNGGKVFISNGWLADAVVVVAVTNREAKTVAHGISLFLVERGMKGFHKGHRLEKIGLKAQDTSELFFEDAIAMTEPGAGSDLQGVRTNAKKDGSDWILNGSKVFITNGWMSDVVIVVAVTNREARTPAHGISLFLVDNGTKGFVKGRKLEKIGLKAQDTAELFFEDVRVPKENVLIGEVNKGFYYLMQELPQERLLISELAISACEFMFEETRNYVKQRKAFGKTVAHIQTVQHKLAELKTHICVTRAFVDSCLQLHEAKRLDSATASMAKYWASELQNSVAYDCVQLHGGWGYMWEYPIARAYVDARVQPIYGGTNEIMKELIARELDS",
    "response#2": "GSGFKIAMGAFDNTRPPVAAGATGLAQRALDEATNYALERKTFGKVIAEHQAVSFLLAEMAMKVELARMAYQRSAWEVDQGRKNTYYASIAKAFAGDIANQVATDAVQIFGGNGFNSEYPVEKLMRDAKIYQIYEGTAQIQRLIIARG",
    "response#3": "SDHDIFRESARKFFQQEVVPYHAEWEKAGQVSRNLWEKAGQQGLLGTSTPEEHGGIGGDILSSAIVWEEQMYVNCTGPGFSLHSDIVMPYISRYGSKSQVEKYIPQMTAGCNTTAXVMAXAXAXGAXXRXDAIAMSEPGAGSDLQGIRTNAKQDGDDWILNGSKVFITNGWMADVVIVVAITNPTAKSAAHGISLFLVEEGMKGFVKGRKLEKIGFKAQDTAELFFEGXIGXEXAIEXALXSXX"
  },
  {
    "reference": "MPSTNPFLTLPYEPAFASLGSEFSDPVEAATFPAHQLRFRNDRLLPILGLDPATVADEHFIEAFGRFQGRSPLLAMRYHGYQFGIYSPDLGDGRGFLYGQVRGRNGWLYDFGTKGSGRTPYSRGGDGKPTLKGGVREVLASEFLQRLGVRTGRCLSLIETGEELWRGDEPSPTRSSVMVRFNRTHIRFGTFERLHYFKRADLVRQLLDHVIATYYSHLLGDPEADAKFYAELTERTADLAAQWMAAGFCHAVLNTDNLSIVGESFDYGPWAFLDRFDPKFTAAYFDHSGRYRYENQPGICQLNLELLQVPLGMVMSAADLEAGIAGFGDRYQATYSRLMLRRLGFEADQLHSAIADDLIITTLQLLLRAPIGYNEFFARLRAQFQPSWRSDLSAILPDWITTDLEALPEAQWQGWRDRYHQLLTHLPESQLPLIQQQLAQANPEISPIRPVVESVWDPIAIDDNWEPLEALLNRWRHDGDGAADGAVVPNAN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The Proton acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.2. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+), ATP binding.3. The protein must be able to magnesium ion binding. The designed protein sequence is ",
    "response#1": "IGILTGGGDCPGLNAAIRAVTRRSIKSYGSTVLGIRNGWAGLINNDTSPLSLVSVSGILHRGGTILGTSRTNPMKDEGNIDRIKENVRDLGLDAVVAIGGEDTLGAAAALNEAGIPMVGLPKTIDNDIVGTDMTFGFDTAVTIATDAIDRLHTTAESHHRVMVIEVMGRHTGWIAIKSGIAGGADCILIPEVPMDLDEVCALVKQRIDRGKTFSLVVVAEGFTMKDTPGQVTQDDQVDEFGHVRLGGIGEVVGAEIERRAGIETRVTILGYIQRGGSPTPYDRVLATRYGVTAADLVHEGKFGRMAALVGDEIVEVPLADAVGELKTVPPHLYEEARTFFG",
    "response#2": "IGILTGGGDCPGLNAAIRAVTRRSIKSYGSTVLGIRNGWAGLINNDTSPLSLVSVSGILHRGGTILGTSRTNPMKDEGNIDRIKENVRDLGLDAVVAIGGEDTLGAAAALNEAGIPMVGLPKTIDNDIVGTDMTFGFDTAVTIATDAIDRLHTTAESHHRVMVIEVMGRHTGWIAIKSGIAGGADCILIPEVPMDLDEVCALVKQRIDRGKTFSLVVVAEGFTMKDTPGQVTQDDQVDEFGHVRLGGIGEVVGAEIERRAGIETRVTILGYIQRGGSPTPYDRVLATRYGVTAADAVEEGAWGVMVALQGTDIVRVPLAEGTGALKTVPVERYAEAEVFFG",
    "response#3": "GFIGGKMPLHHGPAEGEEGLRRNLQELATMRERVGPDFWLMLDCWMSLDVNYATRLAQGAQAHGLKWIEEALPPDDYWGYAALRKNVPTGMLVTTGEHEATRWGFRQLLEMGCCDIIQPDVGWCGGITELLKISALADAHQALVIPHGSSVYSYHFVATRHNSPFAEFLMMAPKADEVVPMFHPQLLGEPVPVNGRMRLSALDRPGFGVELNPN"
  },
  {
    "reference": "MRKINIAILGATGAVGEEFLRLLAERRFPYASIKLLASPRSAGRQVKVGEEVYTVEAVSHDSFKGVDVAFFSAGGDVSLEYAPAAVRAGAVVIDNTSAYRLDPEVPLVVPEVNAEDIAKHKGIIANPNCSTIIMVVALSPLHQAAGIKRVVVSTYQAVSGAGIEAIDELNEQVKAHLEGREITARVLPFASAAKHYQIAFNVIPHIDVFQELDYTKEEWKMVKETQKILHVPDMKVTATTVRVPVYRSHSESINIEFEREMTPELAREVLGKAPGVVVMDDPANMVYPMPLDASGKDPVYVGRIRKDDTVPYGLNIWVVGDQIRKGAALNGLQIAEYMLAANLL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be optimized for NADP binding, aspartate-semialdehyde dehydrogenase activity, protein dimerization activity activity in order to improve diaminopimelate biosynthetic process, lysine biosynthetic process via diaminopimelate.2. The designed protein should be able to compete with endogenous proteins or inhibitors for NADP(+), substrate, phosphate binding.3. The designed protein must have at least one Semialdehyde dehydrogenase NAD-binding domain.4. The protein design should consider the endogenous proteins and enzymes present in the L-threonine biosynthesis, Amino-acid biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "RLSPIESMVVSSYQAVSGAGSAGPQELFGEMEAMAAGKSYVPRVFAHQIAYNVIPQIGERQFVGYTSEEMKLQNEGRRILHLPEMRVSCTCVRVPVVRSHSVSVQLKTREKLTVDDARAAVAAAPGCKLLDELDDLLYPMPLLSTDQDLVLVGRIREDLTDDRGLLLWCCGDQLRRAAAAL",
    "response#2": "LKPIHDEYKIKKVVVSTYQSVSGAGILALDELNNQAHDLNTPPKVLPYAAGKKFYPIAYNLLPQIDNFNNETLYTLEEHKMINETHKILSPSIEVIPTCVRVPVKRGHSESLYIECEKEIDIDKARQLLSKMPNVIIQDDLNEQKYPQPIECEDQDYIYIGRIRPALNNKKALCMWVVADNLGGVASIYRLSPIESMVVSSYQAVSGAGSAGPQELFGEMEAMAAGKSYVPRVFAHQIAYNVIPQIGERQFVGYTSEEMKLQNEGRRILHLPEMRVSCTCVRVPVVRSHSVSVQLKTREKLTVDDARAAVAAAPGCKLLDELDDLLYPMPLLSTDQDLVLVGRIREDLTDDRGLLLWCCGDQLRGL",
    "response#3": "RVAVVGATGMVGQRFVKLLENHSMFELVALTASPASVGKRYSEAAHWYVEGQMPEGVAEMKVSTDDPNWLKALGVEVAFTALPAEVALKVEPELAKAGINVLSDASTFRMEKDVPVLIPEVNPEHLKLLKPLLDNFGVKRVIVSTMQAVSGAGWGGVPSMAIIDNVIPYIAKEEEKVEAESLKILGSLNQGEVKAANFKISASCHRVNVLDGHLEAVFVELGKAVEAVEVINAMKNFKGKPQELKLPTAPQQPIIVKDEPDRPQPRFDRYAGGGMSVAVGRVRRDPAIENGVKYVVLGHNTG"
  },
  {
    "reference": "MEVLSVKAARSMDEDAIKKVGIPSIVLMENAAQEIFNNIVNKGNSFIVFCGAGNNGGDGLAIARKLILSNKRVKVFLVCDLDKASPEFKINYNILINMKAELHRFNIKNDMLTKMIEVVSEADIVVDSIFGIGLNREIKGDIYLVIELINKYAKYVVSVDSPSGLDCDSGGNFGIAIEAKETYSIETLKKGFFQNKAKRYLGEIKVVEIGIPNLVKQRNTENIYLLGENMYKNLIPIRSDYGYKGTYGKVIVLAGSRGLTGAAYITTEAVVRSGAGLTTLLVEEEIKPLLANRLIEAMTVTYNEKEKIDNLIETADVIICGPGLGNGIANRNMLIHCINSSRCPMVIDADGLNIIANDEGCINKLKGRAIFTPHLGEMSRLANESVQKIELHRIDVCINYAKEHGIIALLKGHNTIISNGKDVVINETGNSKMASGGMGDCLTGIIGSLVGQGVGLFDSAVLGAYLHGLAADRLSKDRYVINARDVIEEIPKTIEHLLRK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..2. The protein must exhibit NADHX epimerase activity.3. The protein should have a high affinity for (6S)-NADPHX.4. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "LLVGGSYGKMGAITMSAHAALRAGVGLLTVQVPKTGYNILQTAVPEAMVLTDDHDLHLSQFPDELNKYQCIGIGPGLGQAEDSRLALESLFRQKLPPLVLDADALNLLASDRLLRQQLPPDTILTPHPKEFERLTGPAIDDFHRLQLLQSFCAEHACYVILKGAHTCIGTPSGEFYFNTTGNPGMATGGTGDVLTGILTSLRAQGCPALETCFLGVYLHGLAGDIAAEAEGETSLIASDLYKL",
    "response#2": "LIPVRHPHGHKGTHGRAVLIGGSIGMSGAVILAGKSAQRCGVGLLQIVVPEGIAETVDLGVIEATVWPAKDYEVLTNGSWKVIEERLAGANACAVGPGLKQTELFLPVLKNILKETEVPIILDADALNLISRELDILNLRKGKGTLILTPHPGEMARLCGCSIEDVQENRLKLAVSKALEWGVIVVLKGAGTIVASPDGRVFLNSTGNEGLGTGGTGDVLTGSILSWLAQGVPPLGAACLGVYLHGKAADILKEQYGSSGYTASEVVECLPKR",
    "response#3": "MRSIDANAINNYGIPGIVLMENAALAVVQEIKKILTIEDKKIIKGQKAVILVGKGNNGGDGLAIARHLTVLGMEVTVFLFAGIIELSGDTKLNFELFQKMSGKVISVEDEKQLRLFKLALMQSQIAVDALYGTGFKGSIPENMASYIEDLNKSELPVLCVDIPSGLEADTGKVHNSVVNGDVTVTFGLPKLGHFLGQGPIYTGRLVVDQISIPEYIIR"
  },
  {
    "reference": "MIHAGNAITVQMLSDGIAEFRFDLQGESVNKFNRETLEDFKAAIAAVKADSSIKGLIVTSAKSTFIVGADITEFGENFAKGEQTIVEWAKPVHDIFNSFEDLDIPKVAAINGIALGGGFEMCLVCDYRVMSDKAQVGLPEVKLGIIPGFGGTVRLSRVIGIDNAIEWIATSASKRPAAALKDGAVDAVVSADKLTDAAIDLVKQAISGRLDWKAKRQEKLEPVKLDTLEQMMAFNSAKGVVLAKANPAQYPAPKLLIDSLQASSTLYRDAALDAEAQAFAKAAVTPQAGALIGLFLNDQVVKKASKKHEKGAHPVNQAAVLGAGIMGGGIAYQAASKGTPIIMKDIGNPQLALGMSEANSLLTKQVERGRLKPAQMGETLARIRPSLSYDEFKEVDIVIEAVTENPKVKEIVLAETEKSVRENTILASNTSTISITRLAKALQRPENFVGMHFFNPVHMMPLVEVIRGEKTSEEAIATTVVLAQKMGKTPIVVNDCPGFLVNRVLFPYFGAFDLLLKDGADFQQIDKVMEKFGWPMGPAYLMDVVGIDTGVHGAEVMAEGFPDRMKPDYKGSIQSMYEAKRLGQKNDVGFYKYELDKKGKKAKTVDPTAYEVIAPVVTGEKREFDAQEIIDRMMLAFCNETVRCLEDNIVATPSEADMAMIMGVGFPPFRGGPCRYIDQTGVAEYVALCNKYAHLGKAYEAPELLKDMAANNKKFYG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be involved in fatty acid beta-oxidation by localizing to fatty acid beta-oxidation multienzyme complex and exhibiting enoyl-CoA hydratase activity, 3-hydroxybutyryl-CoA epimerase activity, 3-hydroxyacyl-CoA dehydrogenase activity, delta(3)-delta(2)-enoyl-CoA isomerase activity, NAD+ binding.2. Ideal protein should have a binding affinity for key enzymes in the Lipid metabolism, fatty acid beta-oxidation pathway to enhance the overall efficiency of the pathway.3. The protein must exhibit the following characteristics: Involved in the aerobic and anaerobic degradation of long- chain fatty acids via beta-oxidation cycle; It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.; Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA.4. The For 3-hydroxyacyl-CoA dehydrogenase activity of the protein must be engineered to enable effective catalysis of a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O.5. The designed protein should have a high specificity for NAD(+), substrate binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MVLGAGIMGGGIAYQSALKGTPVLMKDIAEASLTLGMTEAAKLLNKKLERGRIDGLTMASVLSSITPSLHYAGADSVDVVVEAVVENPKVKAAVLADVEAQVSDDTVIASNTSTIPINLLAQSLKRPENFCGMHFFNPVHRMPLVEIIRGEKTSQQTIDRIVAYAAKMGKSPIVVNDDGAGFFVNRILAPYMNEAARLVLAGLGKGDCPGFFVNRVLFPYFAGFSQLMRDIYGASTDQFRVVVHPQSVIHSMVQFQDGSVLAQCVLPMLNEAAYCLQEGIIRSARDGDIGSVYGIGFPPFRGGPFRYMDEIGIGTLVAKLEKQAEKRGDRFQPAPLLVEMAEQQALKVMKM",
    "response#2": "MVLGAGIMGGGIAYQSALKGTPVLMKDIAEASLTLGMTEAAKLLNKKLERGRIDGLTMASVLSSITPSLHYAGADSVDVVVEAVVENPKVKAAVLADVEAQVSDDTVIASNTSTIPINLLAQSLKRPENFCGMHFFNPVHRMPLVEIIRGEKTSQQTIDRIVAYAAKMGKSPIVVNDDCPGFLVNRVLFPYFGAFDLLLKELEAKYPKFEAYAVVLGGGLMGGGIASVTISKAKLPVRVKDISEKGLSNALSYAYKLLDKGVKRRHMSAAQRDKQMALMSTTTEYIGVHDADIVVEAVFEDLKLKHQMVQDIERECTAHTVFASNTSSLPIGQIAAVASRPENVIGLHYFSPVEKMPLVEVIAHEKTSSETIATTVAFARKQGKTPIVVKDCPGFLVNRVLFPYFGAFDLLLKEGFGVKVTSNAVVLGGGLMGGGIASVTISKAKLPVRVKDISEKGLSNALSYAYKLLDKGVKRRHMSAAQRDKQMALMSTTTEYIGVHDADIVVEAVFEDLKLKHQMVQDIERECTAHTVFASNTSSLPIGQIAAVASRPENVIGLHYFSPVEKMPLVEVIAHEKTSSETIATTVAFARKQGKTPIVVKV",
    "response#3": "MVLGAGIMGGGIAYQSALKGTPVLMKDIAEASLTLGMTEAAKLLNKKLERGRIDGLTMASVLSSITPSLHYAGADSVDVVVEAVVENPKVKAAVLADVEAQVSDDTVIASNTSTIPINLLAQSLKRPENFCGMHFFNPVHRMPLVEIIRGEKTSQQTIDRIVAYAAKMGKSPIVVNDDCPGFLVNRVLFPYFGAFDLLLKELGKELGIDGFYVNRVLFPYLAGFSQLVLEGADFVAIDKVMEKQFGWPMGPAYLIDVVGIDTADHCTSVMSVGFPTRMKKVDNDPVSTLYSNERLGQKNGKGFYAVVLGGGLMGGGIASVTISKAKLPVRVKDISEKGLSNALSYAYKLLDKGVKRRHMSAAQRDKQMALMSTTTEYIGVHDADIVVEAVFEDLKLKHQMVQDIERECTAHTVFASNTSSLPIGQIAAVASRPENVIGLHYFSPVEKMPLVEVIAHEKTSSETIATTVAFARKQGKTPIVVKD"
  },
  {
    "reference": "MNRLHTLIDGFSRVRILCIGDVMLDKFLYGSVSRISPEAPVPIMKMDRETRMLGGAGNVVSNLCALGCRTTFISVVGDDGHGRQVRRFLEETHCVPELVECEGYETTVKIRFVAGKHHLLRADQEQPLRMEPELASRFLERVDACLPEADLVLLSDYGKGLFEGRTAPDVIARCRAARKPVIVDPKGADYSRYRGATLVKPNMKEFQEATGVALNPAMDGWEQDAVAGAQRLFSEFGIENLIVTLSEHGMIFIPSSSPSDFVCIPTEARDVFDVSGAGDTSLASLGAALAAGAAVPDALVVSNVAAGIVVGKFGTASVTGTELKEALKEKDIQTPSWRHRDNILTPEAAAELAERFRREKKVVGFTNGCFDLLHLGHLHSFMKAREACDVLFVGLNTDASIKRLKGEDRPINNEEMRSLLLASLDFVDYVVPFDEDTALPLIEKLRPDVIAKEGYPLEFWPEGQFVKSYGGQALELPRLEGFSSTNIINRMKSSPE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. We require a protein that can specifically bind to the metabolites produced in the Nucleotide-sugar biosynthesis, ADP-L-glycero-beta-D-manno-heptose from D- glycero-beta-D-manno-heptose 7-phosphate: step 3/4 pathway.2. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.3. For general function, the protein need meet that Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7- phosphate at the C-1 position to selectively form D-glycero-beta-D- manno-heptose-1,7-bisphosphate.4. The protein's active site must be carefully engineered to ensure that the amino acids involved in ATP + D-glycero-beta-D-manno-heptose 7-phosphate = ADP + D-   glycero-beta-D-manno-heptose 1,7-bisphosphate + H(+) are properly positioned for optimal catalysis.5. A protein with ATP binding, heptose-1-phosphate adenylyltransferase activity, heptose 7-phosphate kinase activity is needed to enhance ADP-L-glycero-beta-D-manno-heptose biosynthetic process in a specific context.6. The designed protein should contain one or more Carbohydrate kinase PfkB, Cytidyltransferase-like domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MVRQTVLCIGDLMLDEFVYGEVGRISPEAPAPVIAVQRSEINVGGAGNVARSIAALGAHCIFVGIVGDDDAARALKAELAKESLIEPMLVVDAARPTTRKVRFVSEHFSTHMLRADWERATPVSAEIETTLIDAITTMLPRADIVLLSDYAKGVLTEKVIRTTIDGARKLGKRVIVDPKNMNFAAYRGASLLTPNRKEFADATRSAARTEVEIVEAARASMRETDGEAMLVTQSEFGMTLVPRQGEAIHVPAHQVKVRDVSGAGDTVVAVLGVALAAGANWDTALRAANAAAAVAVSKQGTATVSLTELRRVIGDAMLDTYFEGTAARLCSEGPVPIVRKTSERHLPGGAANTAANLRALDAQVMLLGFVGRDNAGNLLRAALRERGIDDTWLIEDERASTLHKLRILADGQFVVRFDDEDAHHYAPPGQQLLLARLEEMFPRCDVVIVSDYCYGVISDALIDRLQFLHAAHPNVLLAVDSKNLRRFKHAGATVITPNLLEAQLLVTQNDDMQGLLPAVPEQASTPFPEAERIGQRLLSMIDAEHAVITMAADGVMLVDRQGRARHIPAHPVHHANDIGAGDSFVAAMALSLAAGAQLDEAARIGIDAAEIAVTKRWDVERQRGRTIVFTNGVFDILHAGHVHLLRQAKQLGDVLVVGVNSDQSAQRLKGKHRPINSERDRLALVAALDPVDYVALFDEDTPATLIRTLRPHIHVKGGDYADEQLPEAGAVHEVGGRIVIIPLVGSMSTSSVIDRIS",
    "response#2": "MVRQTVLCIGDLMLDEFVYGEVGRISPEAPAPVIAVQRSEINVGGAGNVARSIAALGAHCIFVGIVGDDDAARALKAELAKESLIEPMLVVDAARPTTRKVRFVSEHFSTHMLRADWERATPVSAEIETTLIDAITTMLPRADIVLLSDYAKGVLTEKVIRTTIDGARKLGKRVIVDPKNMNFAAYRGASLLTPNRKEFADATRSAARTEVEIVEAARASMRETDGEAMLVTQSEFGMTLVPRQGEAIHVPAHQVKVRDVSGAGDTVVAVLGVALAAGANWDTALRAANAAAAVAVSKQGTATVSLTELRREVLVLGDLMLDRYIWGDAERISQEAPVMLLRADRREERLGGASSVSMMLAKLGARVRLAGVVGADEDARRVRALLDSLGVDHEAVLEDSTRPTTVKERYIGRAQQKHPQQMIRVDYETRTPLEPGVEKRLSSALEAALEKIDIVLVSDYDKGVCTPALLARLTQHCRARGIRILADPIRGRDYRKYHGMSAMTPNRLEAGLATGRTLDSVAEAHAAAIQLRDTLDLEAGIITLDSEGMALAPHSGDCLLFPVRPRQVYDITGAGDMVLAVLGLALAAGADYPRAIRLANVAGGLEVEKIGVQALSRQTVLCIGDLMLDEFVYGEVSRISPEAPAPVIAVQRSETNVGGAGNVARNIASLGGRCIFVGLIGEDETGARLKTALAAESGIESRLVRDSARPTTRKVRFVSEHFSTHMLRADWEQAQPATGEIEQQLIDTILPELARADIVLLSDYAKGVLTARVIRNTIDAARKLGKRVIVDPKSLNLAIYRGATLLTPNRKEFAEATRSRADTPGAIAEAAEDAMRLADCEAMLVTQGERGMTLVVRDGETVHVPALPVKVRDVSGAGDTVAAALALSLAANADWETALRVASAAAAVAVGKTGTATATPAELRR",
    "response#3": "MVRQTVLCIGDLMLDEFVYGEVGRISPEAPAPVIAVQRSEINVGGAGNVARSIAALGAHCIFVGIVGDDDAARALKAELAKESLIEPMLVVDAARPTTRKVRFVSEHFSTHMLRADWERATPVSAEIETTLIDAITTMLPRADIVLLSDYAKGVLTEKVIRTTIDGARKLGKRVIVDPKNMNFAAYRGASLLTPNRKEFADATRSAARTEVEIVEAARASMRETDGEAMLVTQSEFGMTLVPRQGEAIHVPAHQVKVRDVSGAGDTVVAVLGVALAAGANWDTALRAANAAAAVAVSKQGTATVSLTELRRALGQRSGPVERELLAEDALLQALSRQTVLCIGDLMLDEFVYGEVSRISPEAPAPVIAVQRSETNVGGAGNVARNIASLGGRCIFVGLIGEDETGARLKTALAAESGIESRLVRDSARPTTRKVRFVSEHFSTHMLRADWEQAQPATGEIEQQLIDTILPELARADIVLLSDYAKGVLTARVIRNTIDAARKLGKRVIVDPKSLNLAIYRGATLLTPNRKEFAEATRSRADTPGAIAEAAEDAMRLADCEAMLVTQGERGMTLVVRDGETVHVPALPVKVRDVSGAGDTVAAALALSLAANADWETALRVASAAAAVAVGKTGTATATPAELRRLSEWRRQGLRVGFTNGCFDILHPGHVKVLTAARGACDRLIVGLNSDASVRRLKGQGRPVQDERARAEVLAALEAVDLVAIFEEDTPINLITAIKPSVLVKGGDYTRAQVVGHEIVEANGGEVLLVDILPGFSTTSLVARA"
  },
  {
    "reference": "MPSGMFSIDNILAARPRCKESLLLPQNGPLLFSSLGESLYGPADYSGFYNRTVAPTSALQGVNGSRLGYNNYYYGQLHLQTPVGPSCCGAVQALGTQQCSCVPSATAYDGAGSVLIPPVPHQMLPYMNVGTLSRTELQLLNQLHCRRKRRHRTIFTDEQLEALENLFQETKYPDVGTREQLARRVHLREEKVEVWFKNRRAKWRRQKRSSSEESENAQKWNKSSKNSAEKRDEQAKSDLDSDS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must be able to DNA-binding transcription factor activity, RNA polymerase II-specific.2. For general function, the protein need meet that Plays a central role in executing Spemann's organizer phenomenon (the dorsal blastopore lip of the early Xenopus laevis gastrula can organize a complete secondary body axis when transplanted to another embryo). The designed protein sequence is ",
    "response#1": "KQPPQVMKTNTRFAASVRWLIGSQLGIHNNPPTVECIIMSEIQSQRFVTRNTQMDNSSLSGQSSGEIQNASSTMEYQQNNHVFSASFRNMQLKKIKRAEKKGTESVMDEKFALFFYTTTTVNDFQIRVWTLSLPVVVA",
    "response#2": "KQPPQVMKTNTRFAASVRWLIGSQLGIHNNPPTVECIIMSEIQSQRFVTRNTQMDNSSLSGQSSGEIQNASSTMEYQQNNHVFSASFRNMQLKKIKRAEKKGTESVMDEKFALFFYTTTTVNDFQIRVWTLSLPVVVK",
    "response#3": "KQPPQVMKTNTRFAASVRWLIGSQLGIHNNPPTVECIIMSEIQSQRFVTRNTQMDNSSLSGQSSGEIQNASSTMEYQQNNHVFSASFRNMQLKKIKRAEKKGTESVMDEKFALFFYTTTTVNDFQIRVWTLSLPVVVI"
  },
  {
    "reference": "MNREDRLYSAAQVRELDRLAIEGQGIAGYTLMQRAAQACWRVLRERWPQARRIAVICGRGNNGGDGYEIARLARAAGLDVKVYAVEALPTQGDGLRACQAWQAERGDIEAFSGALAPADVMVDALFGTGLARAPEGVAAQAIQAINAANAGVLAVDVPSGLDASSGRAFTPTVRAEATVSFIGNKLGLHTGDGPDYAGTRHFDDLQVPEAVYEAFAPQAQRLPQALLAQSLPRRRRGAHKGEHGHVLLIGGNLGMMGAALLAGRAALRCGAGLVSVATRTEHAALLSTAQPELMSRGVRDAIELRPLLARADVVAIGPGLGQDAWARGLWSATLESGKPLIVDADALNLLAAEPVALPDAVLTPHPGEAGRLLGLGNAQVQADRLGALRALEARYGATVLLKGAGTLISGRPPAVCTFGNPGMAVGGMGDVLSGVIAALRAQGLSAEAAAQNGAVLHALAGDRVAAGGERGLLPSDLLVVLRSLANPL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein contains novel YjeF N-terminal domains that confer a unique function or activity.2. The protein should have a high affinity for AMP.3. The designed protein must be optimized for NADHX epimerase activity, ATP binding. The designed protein sequence is ",
    "response#1": "VGRWLQKRNLITHKGNAGHVAILAGSPGKTGAAAMAAMGAVRGGAGLVTLMVPKSLNPILEEKITEPMTLPLDETLTQTVSLSAEEKIISFVSSKQCFALGPGLSLEPEPQYLVRKLIPAVDCPMVIDADGLTAISENVEVLRDARAPVILTPHPGEMARLTGMTVPEIQADRLGVAQTFSREYGVIVVLKGFRTVVASPDGRAAVNTSGNPAMASGGMGDILTGLISAFLAQGMDPFEAACTGVYVHGAAADRVVREKLWGTRGLAATDLLDWIPRIS",
    "response#2": "GGDTGTRGAVLLAARGAQALGAGKVFVATPAGELFDPGQPQLMTADGDAPFDRFDVVVIGCGLGTSARAHARLAAALAQARALVLDADALNLMAADATLATAGVAGLREGHGARRVVMTPHPLEAARLLGCDATHIQADRRGAALELARRFGAVALLKGAGSIVATPEGAWAVVDAGGPALATAGTGDVLAGMIGARLAQGDDAADAACVAAWLHGRAATMWEAARGR",
    "response#3": "PDGHKGNFGHVLVVGGSKGMSGAAALAASAALRSGAGLVTVGCPAGLNDVLEAQCTEAMTASLPETRDGSLAESGVEAALALAGKRDVVALGPGIGRSLQSGAFVRELAMAVGVPLVVDADGLNAFEGASAALQARSASTILTPHPGEAGRLLGTSSGEVNRDRVGAARELARLTGAVVLLKGACTVAASPDGRVIINPTGGPNLATAGSGDVLTGLVAALIAQGLGALEAAALAAFVHGMAGDRIALRRGDAGLLAGELAEELA"
  },
  {
    "reference": "MVNSKRQQRSKKVASSSKVPPTKGRTFTGCWACRFKKRRCDENRPICSLCAKHGDNCSYDIRLMWLEENIYKVRKHSLISSLQARKSKSKPLCQKISKSRFKQMTHFRQLSPPTSDCEDSVHEASKETTLPNDNTFTISVRRLKIYNNAVASVFGSMTNRDYTQKRIDKKLDELLNMVENDISVVNLNCSKHGPYSVFRANPAAVTSALTDQLPSPGHSMSSAEETTTAALSSPPEDSTSLIDIIQGKIFGILWFNCYGNMILNRQEYTTWFINKMRNSLTTEFIRFLGKIIDDPDINMASCLFKECIARWSCVDWQSIAITMLVIIHGYTCPNLTKLLRVWFLQQKLLRFSMYPLVNFIINNTQDLDVLYHCNGLLGNADLFEDPYQDELTSELHVLVTERLVNSWKDTILQQLCSCQDTTLSCSQLRYWQLQLKCNQQFYKDVYAMQD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be optimized for DNA-binding transcription factor activity, RNA polymerase II-specific activity in order to improve thiamine biosynthetic process, positive regulation of transcription by RNA polymerase II. The designed protein sequence is ",
    "response#1": "DRQMTAAMSLIREMSERIHLPRNIQDSASRIFKDVLESKALRGKNNEAQAAACLYIACRKDGVPRTFKEICAVSRVSKKEIGRCFKIIVK",
    "response#2": "DRQMTAAMSLIREMSERIHLPRNIQDSASRIFKDVLESKALRGKNNEAQAAACLYIACRKDGVPRTFKEICAVSRVSKKEIGRCFKIIVR",
    "response#3": "LEQITSADFMSRFCGNLSLPNSIQAAATRIAKCAVDMDLVAGRTPISIAAAAIYMASQASAEKRSAKEIGDVAGAAEITVRQTYKLLYG"
  },
  {
    "reference": "MGSAALPHARQRWVSHALDSNRKFLIANAQMENCAIIYCNDGFCEMFGYSRVEVMQRPCTCDFLTGPDTTKSSIAQLTQALLGSEECKLEILYYRKDTSCFRCLVDVVPVKNEDGVVIMFILNFEDLAQLIAKSSGRSLHHRLSQSWRAGEGRRLKFSLPSLRRLKAQRNSLPTSEFDGVAIDYGKPGGDSLILRDLKTSPKENCVQSETESLLEKERRPSLEADPTLQHPIPKQEPPSLGPRGSYSAWGFIRSRPGGSFHSLRRVSSLDNFEAARSEFQRKFRERRANSEGGMGLSGKASHVKPNPPNSTSDSDLMKYRTISQIPQFTLNFVEFNLEKHRSGSTTEIEIIAPHKVTERTQNVTEKVTQVLSLGADVLPEYKLQAPRIHRWTILHYSPFKAVWDWLILLLVIYTAVFTPYSAAFLLNEEQGEEKHWNCSYSCDPLNIIDLIVDIMFIVDIVINFRTTYVNINDEVVSHPGKIAIHYFKGWFLIDMVAAIPFDLLIFRSGSDETTTLIGLLKTARLLRLVRVARKLDRYSEYGAAVLFLLMCTFALIAHWLACIWYAIGNVERPYMEHKIGWLDNLGDQIGKRYNDSDLSSGPSIKDKYVTALYFTFSSLTSVGFGNVSPNTNSEKIFSICVMLIGSLMYASIFGNVSAIIQRLYSGTARYHTQMLRVKEFIRFHQIPNPLRQRLEEYFQHAWSYTNGIDMNAVLKGFPDCLQADICLHLNRTLLQNCKAFRGASKGCLRALAMKFKTTHAPPGDTLVH",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein contains novel PAS, PAC domains that confer a unique function or activity.2. The designed protein should have inward rectifier potassium channel activity within plasma membrane to regulate membrane repolarization during action potential, regulation of membrane potential, regulation of ion transmembrane transport.3. The protein should have a Helical; Name=Segment S5, Helical; Name=Segment S1, Helical; Name=Segment S6, Helical; Name=Segment S2, Helical; Name=Segment S3 domain that is long enough to span the entire membrane.4. The protein must exhibit the following characteristics: Channel properties may be modulated by cAMP and subunit assembly .; Pore-forming (alpha) subunit of voltage-gated potassium channel.5. The protein should have a high affinity for a nucleoside 3',5'-cyclic phosphate.6. The Extracellular, Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.7. Prioritize Polar residues, Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.8. The protein design must feature the Selectivity filter motif. The designed protein sequence is ",
    "response#1": "IWDWVILCLTFYTAIMVPYNVAFKNKTSEDVSLLVVDSIVDVIFFIDIVLNFHTTFVGPGGEVVSDPKVIRMNYLKSWFIIDLLSCLPYDVFNAFDRDEDGIGSLFSALKVVRLLRLGRVVRKLDRYLEYGAAMLILLLCFYMLVAHWLACIWYSIGRSDADNGIQYSWLWKLANVTQSPYSYIWSNDTGPELVNGPSRKSMYVTALYFTMTCMTSVGFGNVAAETDNEKVFTICMMIIAALLYATIFGHVTTIIQQMTSAVIYCNDGFCELCGYSRAEVMQRPCTCDFLHGPRTQRRAAAQIAQALLGAEERKVEIAFYRKDGSCFLCLVDVVPVKNEDGAVIMFILNFEVQQCERKKRVSIHEHLLNGTGTALNGSSGKLVILPDSMQDLMKLSEKKFGKAARGILTVGGAEVEDIEVIRDGDHLFNDIDNNGYVTKSELFESASRGCLRSLSLIIKTSFCAPGEFLIRQGDALQAIYFVCSGSMEVLKDNTVLAILGKGDLIGSDSLTKEQVIKTNANVKALTYCDLQYISLKGLREVLRLYPEYAQKFVSEIQHELFESASRGCLRSLSLIIKTSFCAPGEFLIRQGDALQAIYFVCSGSMEVLKDNTVLAILGKGDLIGSDSLTKEQVIKTNANVKALTYCDLQYISLKGLREVLRLYPEYAQKFVSEIQRIEEEKARLQRENEELHRQLAYIPGGIISPTEG",
    "response#2": "RIIAIISVMVILISIVSFCLETLPIFRNDDDEPHSVFDTNTNTTIYFTSTYFTDPFFILETLCIIWFSFEFLVRLFACPSKSGFFGNVMNIIDVVAIIPYFITLATELAEKPEDGQAGQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEADEPESQFESIPDAFWWAVVSMTTVGYGDMVPTTIGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRSRLDGQGHGYNLFNGVSGNFIAELVMEVQAEYFPPKEDIILQNEGEADVYIVVSGAVNIITTIHGNEQVYEKIAEGEMFGEVGSLCNIPQPFTCRTAELSQLLRISKTRLREIIEENREDSNILMNNLVQAFRLASDGCLRALAMEFQTVHCAPGDLIYHAGESVDSLCFVVSGSLEVIQDDEVVAILGKGDVFGDVFWKEATLAQSCANVRALTYCDLHVIKRDALQKVLGSDIIPLKDWPIVYSNDGFCKLSGYHRAEVMQKSSACSFMYGELTDKDTVEKVRQTFENYEMNSFEILMYKKNRTPVWFFVKIAPIRNEQDKVVLFLCTFSDENF",
    "response#3": "IWDWVILCLTFYTAIMVPYNVAFKNKTSEDVSLLVVDSIVDVIFFIDIVLNFHTTFVGPGGEVVSDPKVIRMNYLKSWFIIDLLSCLPYDVFNAFDRDEDGIGSLFSALKVVRLLRLGRVVRKLDRYLEYGAAMLILLLCFYMLVAHWLACIWYSIGRSDADNGIQYSWLWKLANVTQSPYSYIWSNDTGPELVNGPSRKSMYVTALYFTMTCMTSVGFGNVAAETDNEKVFTICMMIIAALLYATIFGHVTTIIQQMTSAENCAVIYCNDGFCELCGYSRAEVMQRPCTCDFLHGPRTQRRAAAQIAQALLGAEERKVEIAFYRKDGSCFLCLVDVVPVKNEDGAVIMFILNFFRFKKDRELFQGVSDKLVLPLVAEMKAESFPPKADIILENEASTDCYIIVSGEVEVLTTLEDGTEKQVMRIGPRGMAGEIGVMFNIPQPFTIRSRKLTQLVRISHSHMVSTIRPNTADGVVVFSNFVDRTLKHRNDCLHNSLFESASRGCLRSLSLIIKTSFCAPGEFLIRQGDALQAIYFVCSGSMEVLKDNTVLAILGKGDLIGSDSLTKEQVIKTNANVKALTYCDLQYISLKGLREVLRLYPEYAQKFVSEILAPGDLLYHTGESIDSLCFIVTGSLEVIQDDEVVAILGKGDVFGDQFWKDSAVGQSAANVRALTYCDLHAIKRDKLLEVLQG"
  },
  {
    "reference": "MTDGKLSTSTNGVAFMGILDGRPGNPLQNLQHVNLKAPRLLSAPEYGPKLKLRALEDRHSLQSVDSGIPTLEIGNPEPVPCSAVHVRRKQSDSDLIPERAFQSACALPSCAPPAPSSTEREQSVRKSSTFPRTGYDSVKLYSPTSKALTRSDDVSVCSVSSLGTELSTTLSVSNEDILDLVVTSSSSAIVTLENDDDPQFTNVTLSSIKETRGLHQQDCVHEAEEGSKLKILGPFSNFFARNLLARKQSARLDKHNDLGWKLFGKAPLRENAQKDSKRIQKEYEDKAGRPSKPPSPKQNVRKNLDFEPLSTTALILEDRPANLPAKPAEEAQKHRQQYEEMVVQAKKRELKEAQRRKKQLEERCRVEESIGNAVLTWNNEILPNWETMWCSRKVRDLWWQGIPPSVRGKVWSLAIGNELNITHELFDICLARAKERWRSLSTGGSEVENEDAGFSAADREASLELIKLDISRTFPNLCIFQQGGPYHDMLHSILGAYTCYRPDVGYVQGMSFIAAVLILNLDTADAFIAFSNLLNKPCQMAFFRVDHGLMLTYFAAFEVFFEENLPKLFAHFKKNNLTPDIYLIDWIFTLYSKSLPLDLACRIWDVFCRDGEEFLFRTALGILKLFEDILTKMDFIHMAQFLTRLPEDLPAEELFASIATIQMQSRNKKWAQVLTALQKDSREMEKGSPSLRH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein contains novel Rab-GAP TBC domains that confer a unique function or activity.2. The protein must exhibit the following characteristics: Plays a role in the regulation of starvation-induced autophagosome formation.3. The protein should have a specific function in recycling endosome to Golgi transport and should be localized to cytosol, intracellular membrane-bounded organelle, nucleoplasm in order to carry out this function efficiently.4. The protein should exhibit Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "LKPEGRLHQHGKLTLLHNGEPLYIPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCFLEDFVRWYSPRDYIEEEVTDEKGNVVLKGELSARMKIPSNMWVEAWETAKPVPARRQRRLFDDTREAEKVLHYV",
    "response#2": "VSDGIPKQIRGIIWQLISNSKSKEIRQLYQDLLQIPSEHEKAIQRDISRTKFIPVDKTESLFNVLKAYSLFDPEVGYTQGMAFVTAPLLINVWEEADAFGLLIKLMKNYGLREFFLPDMPGLQLKLYEFDRLLEENSPQLYNHLIRLGIRSSMYATQWFLTLFAYKFPLGFVLRILDVIFVEGIESLLKFAVILMLKNESVLVQLP",
    "response#3": "GLSPGLRREAWKFLLGYLSWESSAEEHKAHVRKKTDEYFRMKLQWKSVSAEQERRNSLLHGYRSLIERDVSRTDRTNKFYEGPENPGLSLLHDILLTYCMYHFDLGYVQGMSDLLSPILFVVQNEVDAFWCFCGFMELVHGNFEESQETMKRQLGQLLLLLRVLDQPLCDFLDSQDSGSLCFCFRWLLIWFKREFPFPDVLRLWEVLWTGC"
  },
  {
    "reference": "MQYFPIFVDTKELNCLVVGAGEVAARKVELLLKTSATITVVAPWACDTVQRLADEGQVTLHTRGYESSDLTAKQLVFVATDDSQLNIDIHHQAKAQNILVNVVDNTPLCQFITPSIVDRSPIIIAMSSGGVAPVLLRYLRQKLESVIPQKVSLLGQFSEKFRETVKARFNSVTKRRYFWEDILDGDVAEQVLQGNNSKAEQMMQDKLALDEHDSGKGEVYLVGAGPGDPDLLTFRALRLMQKADVVVYDRLVSKEILELVRRDAEKIYVGKARSLHTVPQDEINALLADLALKGNRVVRLKGGDPFIFGRGGEEIETLVEKGVSFQVVPGITAASGAASYAGIPLTHRDHAKSVVFATGHLRDNSINLNWPMLAQPEQTTVFYMGLTGLPVICEKLIQHGLPDTTEIALVQSATTTEQKVVAGNLANIQQKVAEAGIKPPALIIVGSVVSLREKLNWFGADA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The desired protein should be able to perform 2 H(+) + siroheme = Fe(2+) + sirohydrochlorin The designed protein sequence is ",
    "response#1": "VGAGPGDAGLLTLKGLQQIQQADIVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHCVPQEEINQILLREALKGKRVVRLKGGDPFIFGRGGEELETLCNAGVPFSVVPGITAASGCSAYSGIPLTHRDYAQSVRLVTGHLKTGSELDWHNLAAEKQTLVFYMGLNQAATIQEKLLEHGMQHDMPVALVENGTAITQRVVSGVLTQLGELAKQVESPALIVVGRVVGAGPGDPDLLTLRALRLMHKADVVLYDRLVSAEIMAKLRRDAQKIHVGKEQANHSVEQETINQMLVRFARDGKKVLRLKGGDPFIFGRGGEELETLTEAGVPFQIVPGITAASGCAAYAGIPLTHRDYAQSVRFVTGHTKEGAVALDWETLAKEQQTLVFYMGLSGLSEICKQLIDNGMEQSMPIALIQQGTTATQKVLVGDLSNMPMLAESKKVQAPTIIIVGRVL",
    "response#2": "VGAGPGDPDLLTFRAMRLMQQADVVIYDRLVAPAILDMVRQDADRIYAGKERNRHTLPQTSINNLLIKLAQEGKRVLRLKGGDPFIFGRGGEEIETLSQHQIPFQVVPGITAASGVASYAGIPLTHRDYAHSCVFVTGHLKDNTVQLDWSALARPNQTIVVYMGLLGVSELCRQLIAHGLPKTTPAAIIQQGTTPNQCVLTGTLATLPTIIQENPLKPPTLIIVGEVL",
    "response#3": "VGAGPGDAGLLTLKGLQQIQQADIVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHCVPQEEINQILLREALKGKRVVRLKGGDPFIFGRGGEELETLCNAGVPFSVVPGITAASGCSAYSGIPLTHRDYAQSVRLVTGHLKTGSELDWHNLAAEKQTLVFYMGLNQAATIQEKLLEHGMQHDMPVALVENGTAITQRVVSGVLTQLGELAKQVESPALIVVGRVVGAGPGDPDLLTLRALRLMHKADVVLYDRLVSAEIMAKLRRDAQKIHVGKEQANHSVEQETINQMLVRFARDGKKVLRLKGGDPFIFGRGGEELETLTEAGVPFQIVPGITAASGCAAYAGIPLTHRDYAQSVRFVTGHTKEGAVALDWETLAKEQQTLVFYMGLSGLSEICKQLIDNGMEQSMPIALIQQGTTATQKVLVGDLSNMPMLAESKKVQAPTIIIVGRVL"
  },
  {
    "reference": "MNGHHQQNGVENMMLFEVVKMGKSAMQSVVDDWIEAYKHSKDVALLDLINFFIQCSGCKGVVSGEMFRHMQNSEIIRRMTEEFDEDSGDYPLTMAGPQWKKFKFSFCEFIGVLVRQCQYSIIYDEYMMDTVISLLTGLSDSQVRAFRHTSTLAAMKLMTALVNVALNLSINMDNTQRQYEAERNKMIGKRANDRLELLLQKRKELQENQDEIENMMNAIFKGVFVHRYRDAIAEIRAICIEEIGVWMKMYSDAFLNDSYLKYVGWTMHDKQGEVRLKCLTALQGLYYNRELNTKLELFTSRFKDRIVSMTLDKEYDVAVQAIKLLTLVLQSSDEVLTAEDCENVYHLVYSAHRPVAVAAGEFLYKKLFSCRDPEEDGIMKRRGRLSPNANLVKTLVFFFLESELHEHAAYLVDSMWDCATELLKDWDCMNSLLLDDPLNGEEALTDRQESALIEILLCTVRQAAECHPPVGRGTGKRVLTAKEKKSQMDDKTHLTELFAVSLPQLLAKYSVDAEKVTNLLQLPQYFDLEIYTTGRLEKHLEALLRQIRNIVEKHTDTDVLEACSKTYHALCNEEFTIYNRVDIAKSQLIDELADKFNRLLEDFLQEEEELDEDDAYQVLSTLKRITAFHNAHDLSRWDLFSGNYKLLKTGIENGDMPEQIVVHALQCTHYVILWQLAKFSETGSSKEELITLKRQMRVFCQICQHYLTNVNTAVKEQAFTILCDVLMIFSHQIVVGGREALEPLVYSPDSSLQSELLSFILDHVFIDQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be designed to localize specifically to nucleus, cohesin complex, chromosome, centromeric region in order to perform its role in cell division, sister chromatid cohesion more effectively.2. The designed protein have SCD domains with specific sequence motifs that are crucial for its activity.3. For general function, the protein need meet that At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate; The cohesin complex may also play a role in spindle pole assembly during mitosis.; The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped; Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication The designed protein sequence is ",
    "response#1": "DYPLTMPGPHWKKFRCNFCEFISVLIRQCQYSIIYDEYMMDTVISLLTGLSDSQVRAFRHTSTLAAMKLMTALVNVALNLSIHQDNTQRQYETERNKIINKRANERLASVYGRLVDLCQPSHKRFNIATTKILQKHMNSIVCDTEETAAKAIVYLKDHRYPPETFLPNDALVVNPLNEKLREIKKPAGVKLVFDVINPQHQAARKALQFVCGNALVCESQEDAKQLASVYGRLVDLCQPSHKRFNIATTKILQKHMNSIVCDTEETAAKAIVYLKDHRYPPETFLPNDALVVNPLNEKLREIKKPAGVKLVFDVINPQHQAARKALQFVCGNALVCESQEDAKQLAARPSRSCRPTSLVEPSLKNKLRAESVYGRLVDLCQPSHKRFNIATTKILQKHMNSIVCDTEETAAKAIVYLKDHRYPPETFLPNDALVVNPLNEKLREIKKPAGVKLVFDVINPQHQAARKALQFVCGNALVCESQEDAKQLAGRPSRQAAEKIKSYKEPSLKEKMVDYLVDSLWESSQELLKDWECMTELLVEEPMQGEEVMSERQESALVELMVCTIRQAAEAHPPVGRGTGKRVLTAKERKTQLDDKTKLTEHFIVALPVLLSKYSADAEKVANLLQIPQYFDLELYSTGRMEKHLDSLLKQIRFVVEKHIESDVLEACSKTYSILCSEEYTIQNRVEIAHSQLIDELADRFSHAVEELLQEAEEADEDEIYNVMASLKRLTCFHNAHDLTKWDFFGNCYRLLRAGIEHEGMMEQIVVQALQCSHYSIL",
    "response#2": "YLVDSLWDCAGSYLKDWESLTNLLLQKDQNLGDMQERMLIEILVSSARQAAEGHPPVGRITGKKSLTAKERKLQAYDKMKLAEHLIPLLPQLLAKFSADAENVAPLLQLLSYFDLSIYCTQRLEKHLELLLQQLQEVVVKHVEPEVLEAAAHALYLLCKPEFTFFSRVDFARSQLVDFLTDRFQQELDDLMQSSFLDEDEVYSLTATLKRLSAFYNAHDLTRWEISEPCSRLLRKAVDTGEVPHQVILPALTLVYFSN",
    "response#3": "YLVDSLWESSQELLKDWECMTELLLEEPVQGEEAMSDRQESALIELMVCTIRQAAEAHPPVGRGTGKRVLTAKERKTQIDDRNKLTEHFIITLPMLLSKYSADAEKVANLLQIPQYFDLEIYSTGRMEKHLDALLKQIKFVVEKHVESDVLEACSKTYSILCSEEYTIQNRVDIARSQLIDEFVDRFNHSVEDLLQEGEEADDDDIYNVLSTLKRLTSFHNAHDLTKWDLFGNCYRLLKTGIEHGAMPEQIVVQALQCSHYSILV"
  },
  {
    "reference": "MKIFTTKQIAAIDQFTIQQEPIASIDLMERAALQIANWLIHHLSNERKIIFFAGPGNNGGDALAVARIMAEHDFICEVFLVDFGKPLQAEPAENWHRLKEQGKVKLGRIESQESIPAVPDDAVVVDGLFGSGLSRKLDGLPLQIAAALNASRALVVAIDIPSGLFGEDNSNNDLGAVIQADYTLTLQFPKLSFFFPEHEALLGRWEVLPIGLHPSVISETETPFHFLTAEEISDKIPVRKKFSHKGTYGHALLIAGSYGKVGAALLASKACLRSGVGLLTAHIPHSAYQIIQTAVPEAMCSIDASDLMFTEFSDLQQFSAVGAGPGLGVKPNTRKALLRLLEASPAKLVLDADALNILAMHPEWLPLLPENSILTPHPKEFERLVGPSGDSYSGLQSQLAFSQKYKVIVVLKGAHTRITSPDGRVFFNTTGNPGMATAGSGDVLTGIVLGLLAQDFEPLDAARVAVYAHGLAGDLATVQCDELSLIASDIIENLGEAFFQIRK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must possess NADHX epimerase activity, NADPHX epimerase activity, metal ion binding, kinase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity to support phosphorylation, nicotinamide nucleotide metabolic process under certain conditions.2. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration3. The presence of Mg(2+) is necessary for the protein to function in the desired environment.4. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.5. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.6. The AMP, (6S)-NADPHX, K(+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MRAIDRATIDEIGLPGAVLMETAGRAVAEVVRARRRAGATVAVVCGAGNNGGDGYVAARVLREWGYAASAYLAADRAKVAGDAALHLRVLEQCGGRVASIATEADLLEHERAIERADVVVDALFGTGLARDVTGHYARVVAVLNRARGFKVAVDIPSGLAADTGAVLGVAFNADATVTMAFDKPALVSSPGFARCGEVTVAEIGIPAELAGPLAHHQEVAALLPVPGPESDKYRRGVVGVAAGSERYPGAAVLAVAGALRGGAGAVRYVGPPAEAVLARFPEVLIGPGRVQAWVAGSGLGDAPEARERLARALDDGVSTVVDADGLRMLGPAPGRTARGPLVLTPHAGEAAALLGVAREEVEEARLESVRQLARQTGATVLLKGSTTLIASPPGEADAGEEDGGTVLANLNGTGWLATAGTGDVLAGLIGSLLAAGLAPADAAAVGAHLHGLAGSRFPSRSFASHKGTYGSLAVVGGDTGMCGAPILAARAALHTGAGKVHVAFIGEGAPPYDPPFPELMLHPPGTLGFGLEAMDALAIGCGMGHGPRAVSSLKQAIASDLPLLLDADALNLLASETALADALAARAVIDVDGNADAESGGTACACILTPHPLEAARLMGCDAAQVQRDRIGTARALAARFGAVVVLKGAGTVIASPDGRAAVNPTGNAGLATGGTGDVLGGIVGALLAERLPSYEAALAGVYLHGWAADTLTASGRGPAGLTAGELAPVVRDLL",
    "response#2": "MAELDRKTIEEIGIPGIVLMENAARGAAAFFEAVIPDLARRHIVVLAGSGNNAGDGFALARIFHGKGAPVRVVCLRPAHRLKGDALINYNILHRLGIPVYHWDEREDFALQFAHVQKADVILDALLGTGLQSEVRGLYREVIDAVNALGKPVLSVDVPSGLHAGTGRVLGTAIKATATATFGLLKVGHVMGEGPARSGRVQVVDIGIPPAVTPGASSLVGVAAGSERYPGAAVLAVAGALRGGAGAVRYVGPPAEAVLARFPEVLIGPGRVQAWVAGSGLGDAPEARERLARALDDGVSTVVDADGLRMLGPAPGRTARGPLVLTPHAGEAAALLGVAREEVEEARLESVRQLARQTGATVLLKGSTTLIASPPGEADAGEEDGGTVLANLNGTGWLATAGTGDVLAGLIGSLLAAGLAPADAAAVGAHLHGLAERDDAAAALPATTPVDHKGRRGHALVIAGSPGKWGAGRMAARAALRAGAGLVTLAGPRADAAAPDEVMTAAIDPEAAGFDVALAELLAGKRAVAIGPGMPATAAGAALVRAVLERAEVPVVIDADALNHMAGDLAAVAAARAPVVLTPHPGEAGRLLGCSAADIERDRIAAVRRLAERTRAVVVLKGARTLVCDGAVDGFTTVNPTGNAAMATAGAGDVLTGVIVALAAQGAPPADAARAGVFLHGLAGDRAARGRIDAGLMAGDLIDELPRARADLTPRG",
    "response#3": "MRNIEERIFSAGIPVAALMEKVAGKINHRLQTLFSEKSITSADKIGILVGPGHNGGDGLVVARELYFLGYNIVIYSPISKPKELTESHAKYAISLGINFSSNIKELYDCDILIDGLFGFGLEREITGKIANAINIINNLNKFVISIDLPSGIHTDTGEILGTAIRANITLCLGLWKQAFLQDTGLDYIGRAELIDFDIPRADIGIVAGSRGFAGAATMCAWGALRAGAGLVEVFVPENIYEVVAASAPFESMVKPVTSYRDLLDEKIDVWGIGPGLGKENAREIIDLVQRTKNAMVVDADALNLISREVSILKNCRGPRLLTPHPGEMKRLAGDRKISRAKLAKNFTAEFPVTLLLKGSRTVVAENGKRISYNTTGNPGMATGGMGDVLTGVCASLIAQNLSPFDAARVGAWVCGRAAELAIS"
  },
  {
    "reference": "MVTSLVRKIFGSRNERIVKRLGKTVARINELEAELQSLDDEALKARTGQLRERLAGGESLEALLPEAFAVTREAGRRVMGMRHFDVQLIGGMVLDSGRIAEMRTGEGKTLVATLAAYLNALSGKGVHVVTVNDYLARRDAAWMGRLYHALGLSVGVINSSGGAGPDSASYLYDPGFHAEGGIAHLRPVTRREAYAADITYGTNNEFGFDYLRDNMAFRLEDRVQRELNFAIVDEVDSILIDEARTPLIISGPAGESAEMYERMNRIVPKLTPQEEEEGPGDYSVDEKMKQVFLTEDGQEKAEQLMRDAGLLAEGQGLYDAGSIALLHHLNAALRAHILFHKDVDYLVRDGQILIIDEFTGRIMAGRRWSEGLHQAIEAKEGVPIQRENQTLASITFQNYFRLYEKLSGMTGTADTEAYEFQQIYGLEVVVIPTNRPMVRNDMQDLVYMTQKEKFEAIIKEIKYCQEKRQPVLVGTASVETSEYLSGLLKKAKIAHEVLNAKQHEREAHVVEQAGRPGAVTLATNMAGRGTDIVLGGSLEAELATLGDNPKPADVDRVKADWQKRHDEVLANGGLHIIGSERHESRRIDNQLRGRAGRQGDPGSSRFFLSLEDNLMRIFASDRVKSLMQRLGMQEGEAIENAWVTKAIENAQRKVEAHNFDIRKNLLEYDDVANDQRKVVYEQRRELLETEDISETLEAVRRDVLEGVISQYIPQGSIEEQWDVPGLTHVLEQDFGLVLDIAGWLEREDDLHEETLRERIHQHAAEAYQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. We are looking for a protein with broad substrate specificity for ATP + H2O + cellular proteinSide 1 = ADP + phosphate +   cellular proteinSide 2..2. The protein must exhibit the following characteristics: Part of the Sec protein translocase complex; Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.; Interacts with the SecYEG preprotein conducting channel.3. The protein should exhibit Basic and acidic residues compositional bias.4. The ATP, Zn(2+) binding site should be stable and able to withstand changes in environmental conditions.5. The protein requires Zn(2+) to activate a critical catalytic site.6. The designed protein should be involved in protein import, protein targeting by localizing to plasma membrane, cytoplasm and exhibiting ATP binding, metal ion binding. The designed protein sequence is ",
    "response#1": "IGGMVLNAGQIAEMRTGEGKTLTATLPAYLNALPSKGVHVITVNDYLAKRDAETNRPLFEFLGMTVGVNVANMAPPEKKEAYQADILYGTNNEFGFDYLRDNMAFRAEDRVQRERFFAVVDEVDSILIDEARTPLIISGPAEDSSDLYTRINTLIPSLTXVAIEHPWVTKAIENAQRKVEGRNFDIRKQLLEYDDVANDQRKVVYELRDELMNVDDISEMIGYNRQEVLEGLFGQYIPPQSLEEMWDVEGLTIRLRADFDLDLPLQEWLDNDDKLHEDNLREKIIEAAVQVYKEKEESVGESVLRNFEKAVMDDYRRALDFCRQLRDGLHFRIFAADKLIQLTPAGRDHITNICGNLPGVWQVARARFELIEQALAAQLLYMRDKHYIVKDEKVQIVDEYTGRVMSDRTWESGLHQMISSELYIKVNKIIPNLIRQEKEDSDSFQGEGHFSVDEKARQVHLTERGLVAVEELMVSEGIMAEGESLYSPGNIMMMHHVTAALRAHVLFTRDVDYIVKDGEVIIVDEHTGRTMQGRRWSDGLHQAVLSSSSQLYLQINALIPRLKRHIEEEEGVVTQEGHYVVDEKTRQIELNEQGHQFIEELLASAGLLPEGDNLYSAHNLQLLTHVYAGLRAHVLFHRNVEYIVQGNQVLLIDEHTGRTMQGRRLSEGLHQAILSSGWLAASLQAEDSSQLYLQINALIPRLKRHIEEEEGVVTQEGHYVVDEKTRQIELNEQGHQFIEELLASAGLLPEGDNLYSAHNLQLLTHVYAGLRAHVLFHRNVEYIVQGNQVLLIDEHTGRTMQGRRLSEGLHQAILSSGWLAASLT",
    "response#2": "MGGAALHFGNIAEMMTGEGKTLACVMPAYLNAIGGKGVHIVTVNDYLAGRDAEQMGRVHRFLGLTVGVILSEMKPDERRAAYAADITYGTNNEFGFDYLRDNMAGRLEDRVQRGHCYAIVDEVDSILIDEARTPMIISGPADDATRNNKIYDLINRTQLICEGSHFSMREYNLKLDDVINDQRNVVYKLRNNLLNEEVNMIEIVIPMIKNTLTVIAKDHLLEGMLPEEWDFTRLVEDLKAVLSTEEIPALSANNVHSAEDLQELLKDTLTSYIERVNALESDADAQQVLRQISLHFLDSGWTSHLSAMQHLKEGIGLRQYQQEDPARLYQKEGFEIFLKKSSSSDFHHLCAKVMKTFQDTLHYTYDAETKACNFTEDGITKIEDLFDIDNLYDLEHQTLYHYMIQALRAHVAFQLDVDYIIEDEKIMLVDIFTGRIMDGRSLSDGLHQALSSKLYTEINRLIPKLEQHIEEVEGEVTKPGHFTVDEKTRQVELNEAGHQFIEEMLTEVGLLAEGESLYSAHNLGLLTHVYAGLRAHKLFNRNVEYIVADGQVLLVDEHTGRTMPGRRLSEGLHQAILAEEGKPIEAKVVSRAIRNAQSQVEQANFDMRRSVVKYGQVLDQQRRIVYQARSRLLEGEDMQHQIFHMIGDVVTAYVNECTAGRRTADWDLETLRAALSTLYPVVWQPDPRPHMRGLTRSVLRHEVIADARRALVRRKAAIEARSGLRVMRELERAILLDCLQAEDSSQLYLQINALIPRLKRHIEEEEGVVTQEGHYVVDEKTRQIELNEQGHQFIEELLASAGLLPEGDNLYSAHNLQLLTHVYAGLRAHVLFHRNVEYIVQGNQVLLIDEHTGRTMQGRRLSEGLHQAI",
    "response#3": "MESKLLTKVIASAQAKVEAHNFEGRKNLLQYDDVANEQRKAIYEQRNYLLETNDISAMIETIRDDVFNHVISRYIPPQSIEEMWDIAGLEEVLHHQFGMELPIQQWLEKEKDLHEETLRERIINLAKQEYQSKEEKVGAEVMRNFEKGVMSSQLYLQINALIPRLKRHIEEEEGVVTQEGHYVVDEKTRQIELNEQGHQFIEELLASAGLLPEGDNLYSAHNLQLLTHVYAGLRAHVLFHRNVEYIVQGNQVLLIDEHTGRTMQGRRLSEGLHQAIFETDAAPLLRVLFGSKNDREVKRMRRAVRAINALEEQMVALTDEQLRAKTEEFRGRLGKGETLDQLLPEAFAVAREAGKRVMGMRHFDVQLIGGMVLHEGKIAEMRTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLARRDANWMRPLYEFLGLSVGVVTPFQPPEEKRAAYAADITYGTNNEFGFDYLRDNMAFSLEDKFQRELNYAVIDEVDSILIDEARTPLIISGQAEDSSQLYLQINALIPRLKRHIEEEEGVVTQEGHYVVDEKTRQIELNEQGHQFIEELLASAGLLPEGDNLYSAHNLQLLTHVYAGLRAHVLFHRNVEYIVQGNQVLLIDEHTGRTMQGRRLSEGLHQAIEAKEGLPIQAESQTLASTTFQNYFRLYHKLAGMTGTADTEAFEFRQIYGLDVVVIPTHRPIARKDFNDLVYLTQEEKYAAIIGDIKECQTQGRPVLVGTASIESSEYVSQLLKKEGIAHQVLNAKYHEKEAEIIAQAGRPGAVTIATNMAGRGTDILLGGNWEVEVAALENPTDEPVAQIKADWQKRHQQVIEAGGLHVIASERHESRRIDNQLRGRAGRQGDPGSSRFYLSLEDNLMRIFASDRVKNFMKALGMQAGEAI"
  },
  {
    "reference": "MTDKIYALDLDNGEWSQILDKEFGEPRFRADQICQWIWQKRTFDVAEMTNLSKALRDMLESKIDFGAPLLIKEQRSSIDGTRKYLWQMRDGQSVESVLMRQGERLTACISTQVGCPLGCTFCATGLSGYVRNLSAGEIAGQFLAMERSVGRDINNVVYMGMGEPFLNTEAVLKSVRMLNDPDMRNLGIRHIAISTSGIVPGIRELAASGLGVRLAVSLHAAEDKLRSTLMPVNETFPLDDLRKAMQEYQKTTGNRITIEYALLGGINDSVEHARLLLRYLKGIHVFINLIPFNQVDVRYEKPAPENILKFRSVLVTAGFEAEIREELGSDIDAACGQLRRKTMAGDPCMLEPGPKVKPAYEAKKKEVRTSQREASDPRRTSGPSRSKSGSDAAGQKREKTERAGTKSSGGFVEERGAKRPASEDKRRKTDAPPKERYRSGKMKEERPVYRAKPKANDADHALETDEKRKSGTTRAAKRPSYRAKSEGQSPDRTSKTDEKKKSGTTRAAKRPSYRAGSEGKSADRPSRTDEKKKRSINKSAKKPVAAGGNGRKKPAAESRKKKPRTAAKDNRQKRS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A key design requirement for the protein is the optimization of the S-methylcysteine intermediate, Proton acceptor to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.2. The ideal protein would be one that plays a critical role in rRNA base methylation through tRNA (adenine-C2-)-methyltransferase activity, tRNA binding, 4 iron, 4 sulfur cluster binding within cytoplasm.3. The designed protein should have a high specificity for S-adenosyl-L-methionine binding, with minimal off-target effects.4. The protein should exhibit Basic residues compositional bias. The designed protein sequence is ",
    "response#1": "NTVCVSSQVGCPMGCLFCATGKLGFKRNLTADEIVEQVLYFARQLKKENKRVTNIVFMGMGEPMVNYDNVMAAIRILNNPNGFNLGARRMSISTSGIPEGIRKFAREGLEVNLALSLHAPNNELRCKIMPVSRRHSLEEVMDALREYVDITHRKIMFEYIMIKDVTDTPELAHELVKLLSGWLGYVNLINYNQTEMFAPSTRERIEEFKKRVEVK",
    "response#2": "SSQVGCPLRCAFCATGQMGFRRNLATSEITDQAFLLDAEALKRHGRGLSNIVFMGMGEPLLNLDNVFESIGTLTEQDYRFSISEKKITISTVGLPAEMERIARSGLKTKLAVSLHSADQSVRERMMPVALDVPLDKLAKAISICNTITGQPVTLVYMLLEGVNDSPEDARKLYRQAADAEEDRGTLCVSSQVGCTLTCSFCHTGTQRLVRNLTAGEIVGQVMLARDDLGEWPVPGAPKVETRLLSNIVLMGMGEPLYNFEAVRDAMRICMDGEGIALSRRRITLSTSGVVPEIARCATEIGCLLAVSLHATTDEIRDRLVPINRKWNIETLLAALRDYPRLSNSERITFEYVMLDGVNDSDEDARRLVRLIAGIPAKINLIPFNEWPGAPYRRSPEARIRRFADIIHKAGYASPIRDSRGASLEQSLGVMVGMGQKDSCVGMAADDAPRAVFPSVAGRPRHTGVMVGMGQKDSFCGDDAPRAVFPSVMGRPRHQGVMVGMGQKDSFQGAMMKILTERGYTMTTTAEREIVRDIKEKLCYVALDFEQEMETAEQSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETAYNSIMKCDVDIRKDLYGNIVLSGGTTMYPGIADRMQKDITALAPSTMKIKVVAPPERKYSVWIGGSILASLSTFQQMWLGRSESELSGPAGGKGCGSQAPWGCGSQAPWGCGSQAPWGCGSQAPWGCGSQAPWGCGSQAPWGCGSQAPWGCGSQAPWGCGSQAPWGCGSQAPWGCGSQAS",
    "response#3": "GGDRRTVCVSTQVGCPVGCPFCATGAMGFVRNLTPGEIAAQVLHFARELREAGEHVTHVVVMGMGEPLLNYDSTLKAIHNLNEGRGFRLGARRITVSTVGVVPGILRLAKEDLQVNLAISLHAPDDALRRKLVPLGERWPIAEVLAAADRYAHATGRRVSYEYVLLAGVNDHLDDARALAALLRGRLAHVNLIPFNPSPGLPFRAPAAGTVDAFRRALVESGVDATVRRSRGVAIKTEPAYRLKQAKQAVFRDLISNWQETMVLPAVLREELNQKCPLEIKGEVFASKTGDSLKAIISLRDGLKIESVLMKHADGRNTVCVSSQVGCPLGCSFCATGKLGWKRNLEPIEIAEQALFFSRHLKEKGEKVSNVVFMGMGEPFLNYENVLAAIKILNDKDGFNLGGRHLSISTVGIIEGIKKLAEEKLEINLAISLHAPNDELRSRLMPINKKYPLKKVLTAVDEYLKKTRRRVMFEYIMIKGVNDSEAQAEELAKLMKKPLYFINLISYNPARIGFDNPTGVFTPSSPAAIKRFRQVLEKRGVAVTQRHRFGEDIDAACGQLVYRRKEASA"
  },
  {
    "reference": "MRDRVALIVAAGRGTRFGGPAPKQYLPFGDTNVLAAAAMALRPFMPVACVIHPDDRARYGSATAGLDLLAPVPGGPDRQSSVRNGLEALAGDPPEFVLVHDAARPDLPAAVVRRILEALATHDGAAPGLPVADTLKRVEDGRSVGTVSRDGLWRIQTPQGFRFSALLEAHRQAAPAAFSDDTALAEAAGLDIAVVQGDARLEKITGPDDAARLSGPDIRTGHGFDVHRLVPGAGVTLCGVRIPHDMALSGHSDADVGMHALTDAIIGALAEGDIGRHFPPDDPEWQGAASEIFLAHARDMAAARGGRILHCDVTLVCEAPRIGPHRDAMRAVLARILAIPVDRVSVKATTSEKLGFTGRGEGIAAQATATLALP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. a divalent metal cation is required for the proper folding and stability of the protein.2. The desired protein should be able to perform 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-   erythritol 2,4-cyclic diphosphate + CMP3. The protein design should be able to enhance the efficiency of terpenoid biosynthetic process.4. The protein contains novel 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domains that confer a unique function or activity.5. The designed protein should interact with enzymes and substrates within the isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6 pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "RMGQGYDVHAFGPGDHVWLGGHKVAHTKGLVGHSDADVLSHAITDALYGAIGDGDIGSHFPPSDPKWKGADSSIFLAAAAEAVRARGGMIAHIDATVVCENPKVGPHRDAIRASIAEVVKLPLDRVAVKATTSEKLGFTGREEGIATLAIATGR",
    "response#2": "DGSEDNFKVTTPEDFERAERFLSASNETRSGSGYDVHRFTDGDHITLCGVAIPHTHSLLGHSDADVGLHAITDALLGAISAGDIGDHFPPTDPKWKGAPSRVFLEHAATLLAGQNGRISNVDVTLICEAPKIGPHREAMRNAIAEIVSVSPDRVSVKATTTEGLGFTGRGEGIAAQALVTPRVGIGTDVHAFGGEGTLWLAGVEWPGEQALHGHSDGDAVAHAIVDAVLAAAGLGDIGEHFGIDQPQYAGAHAAAFLARTRHLVESAGFRIGNVSVQVQARRPRFATRRAEAERVLTAALGAPVSVSATTTDGLGFTGTGEGVAAFAVALVHR",
    "response#3": "RMGQGYDVHAFGPGDHVWLGGHKVAHTKGLVGHSDADVLSHAITDALYGAIGDGDIGSHFPPSDPKWKGADSSIFLAAAAEAVRARGGMIAHIDATVVCENPKVGPHRDAIRASIAEVVKLPLDRVAVKATTSEKLGFTGREEGIATLAIATGRY"
  },
  {
    "reference": "DPGCLPDWSSYKGHCYKVFKKVGTWEDAEKFCVENSGHLASIDSKEEADFVTKLASQTLTKFVYDAWIGLRDESKTQQCSPQWTDGSSVVYENVDEPTKCFGLDVHTEYRTWTDLPCGEKNPFICKSRLPH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Snaclec that binds to von Willebrand factor (VWF) and induces its interaction with GPIbalpha (GP1BA) (via the vWF A1 domain), resulting in platelet aggregation.2. The protein can be expressed in extracellular region and perform toxin activity without triggering undesirable cellular responses.3. The intended protein structure should align with strand-strand-helix-strand-helix-strand-strand-strand-strand-helix-strand-strand criteria. The designed protein sequence is ",
    "response#1": "LQCYNCPNPTADCKTAVNCSSDFDACLITKAGLQVYNKCWKFEHCNFNDVTTRLRENELTYYCCKKDLCNFNEQLENGGTSLF",
    "response#2": "EMTWADAEKFCTQQHTGSHLVSFHSTEEVDFVVKMTHQSLKSTFFWIGANNIWNKCNWQWSDGTKPEYKEWHEEFECLISRTFDNQWLSAPCSDTYSFVCKC",
    "response#3": "RTLLLGWVFAQVAGASGTTNTVAAYNLTWKSTNFKTILEWEPKPVNQVYTVQISTKSGDWKSKCFYTTDTECDLTDEIVKDVKQTYLARVFSYPAGNC"
  },
  {
    "reference": "MDKLESTQNSSDILYPECHLNSPIVKSKIAQLHVLLDINQPYDLKDNSIINITKYKIRNGGLSPRQIKIRSLGKILKQEIKDIDRYTFEPYPIFSLELLXLDIPEICDKIRSIFSVSDRLIRELSSGFQELWLNILRQLGCVEGKEGFDSLKDVDIISDITDKYNKNTWYRPFLTWFSIKYDMRWMQKNKSGNHLDVSNSHNFLDCKSYILIIYRDLVIIINKLKLTGYVLTPELVLMYCDVVEGRWNMSSAGRLDKRSSKITYKGEELWELIDSLFPNLGEDVYNIISLLEPLSLALIQLDDPVTNLKGAFMRHVLTELHTILIKDKIYTDSEADSIMESLIKIFRETSIDEKAEIFSFFRTFGHPSLEAITAADKVRTHMYSSKKIILKTLYECHAIFCTIIINGYRERHGGQWPPCEFPNHVCLELKNAQGSNSAISYECAVDNYSSFIGFKFLKFIEPQLDENLTIYMKDKALSPRKAAWDSVYPDSNLYYKVPESEETRRLIEVFINDNNFNPEDIINYVESGEWLNDDSFNISYSLKEKEIKQEGRLFAKMTYKMRAVQVLAETLLAKGVGELFSENGMVKGEIDLLKRLTTLSVSGVPRSNSVYNNPILHEKLIKNMNKCNSNGYWDERKKSKHEFKAVDSSTEGYETLSCFLTTDLKKYCLNWRFESTALFGQRCNEIFGFKTFFNWMHPILEKSTIYVGDPYCPVSDRMHKELQDHDDAGIFIHNPRGGIEGYCQKLWTLISISAIHLAAVKVGVRVSA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The presence of Mononegavirus-type SAM-dependent 2'-O-MTase domains is critical for the interaction of the designed protein with its intended targets.2. The protein must exhibit the following characteristics: The viral phosphoprotein acts as a processivity factor; Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides; The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency; The first gene is the most transcribed, and the last the least transcribed; RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation; Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes.3. The protein should be optimized for virion component-specific folding and stability, while maintaining ATP binding, mRNA (guanine-N7-)-methyltransferase activity. The designed protein sequence is ",
    "response#1": "MSTFLTTDLQKFCLNWRQETSNIFAERLNEIYGLPRFFNWMHKRLELSILYVADPHCPPFNTDHIFLDDVPNDQIFIKYPMGGIEGYCQKLWTISTIPFLFLSAYEVGTKIAAVVQGDNQAIAITRRVHPNLSYRTKKIKSTEMAQKYFNQLRLNMAAIGHNLKANETIVSSHFFVYSKRIYYDLRTFGKLPYPTRNVQTLCEALLADGLAKAFPSNMMVVTEREQKESLLHQASWHHTSDDFGEHATVRGSSFVTDLEKYNLAFRYEFTAPFIEYCNRCYGVKNVFNWMHYTIPQCYMHVSDYYNPPHNLTLENRDNPPEGPSSYRGHMGGIEGLQQKLWTSISCAQISLVEIKTGFKLRSAVMGDNQCITVLSVFPLETDADEQEQSAEDNAALRGSSFVTDLEKYNLAFRYEFTAPFIEYCNRCYGVKNVFNWMHYTIPQCYMHVSDYYNPPHNLTLENRDNPPEGPSSYRGHMGGIEGLQQKLWTSISCAQISLVEIKTGFKLRSAVMGDNQCITVLSVFPLETDADEQEQSAEDNAARSAGFVNLDIVKYCLSQRLESNALIFSVMDKLLGLEKFFRWHHLYMSGRTACVTDPFCPPFQTKYIGLDEQPNEGIFIKNPAGAVEGYQQKAWTMISVTDLNIVGQEMGTEIFAVAQGDNQSIAVTVKNSKDKNVVDCKKSALEESIRFKYHLQKLLIKKD",
    "response#2": "MSTFLTTDLQKFCLNWRQETTNIFAQRLDEIYGLPGFFSWLHKRLEKSTLYVSDPNCPPYNDVRLGIDDVENRQIFIKNPMGGIEGYCQKMWTIITIPLLFLSAYECGAKIAAVVQGDNQAIAITKRVHPGGLGGSGLLGGLFGGGRRGSSFVTDLEKYNLAFRYEFTAPFIEYCNRCYGVKNVFNWMHYTIPQCYMHVSDYYNPPHNLTLENRDNPPEGPSSYRGHMGGIEGLQQKLWTSISCAQISLVEIKTGFKLRSAVMGDNQCITVLSVFPLETDADEQEQSAEDNAARSAGFVNLDIVKYCLSQRLESNALIFSVMDKLLGLEKFFRWHHLYMSGRTACVTDPFCPPFQTKYIGLDEQPNEGIFIKNPAGAVEGYQQKAWTMISVTDLNIVGQEMGTEIFAVAQGDNQSIAVTVKNSKDKNVVDCKKSALEESIRFKYHLQKLLIKKV",
    "response#3": "MSTFLTTDLQKFCLNWRQETSNLFAERLNEIYGLPGFFNWMHKRLEKSVLYVADPHCPPYNKEHIELDSVKNEQIFIKYPMGGIEGYCQKLWTITTIPFLFLSAYERTFGKLPYPTRNVQTLCEALLADGLAKAFPSNMMVVTEREQKESLLHQASWHHTSDDFGEHATVRGSSFVTDLEKYNLAFRYEFTAPFIEYCNRCYGVKNVFNWMHYTIPQCYMHVSDYYNPPHNLTLENRDNPPEGPSSYRGHMGGIEGLQQKLWTSISCAQISLVEIKTGFKLRSAVMGDNQCITVLSVFPLETDADEQEQSAEDNAARTFGKLPYPTRNVQTLCEALLADGLAKAFPSNMMVVTEREQKESLLHQASWHHTSDDFGEHATVRGSSFVTDLEKYNLAFRYEFTAPFIEYCNRCYGVKNVFNWMHYTIPQCYMHVSDYYNPPHNLTLENRDNPPEGPSSYRGHMGGIEGLQQKLWTSISCAQISLVEIKTGFKLRSAVMGDNQCITVLSVFPLETDADEQEQSAEDNAAFKRTFGKLPYPTRNVQTLCEALLADGLAKAFPSNMMVVTEREQKESLLHQASWHHTSDDFGEHATVRGSSFVTDLEKYNLAFRYEFTAPFIEYCNRCYGVKNVFNWMHYTIPQCYMHVSDYYNPPHNLTLENRDNPPEGPSSYRGHMGGIEGLQQKLWTSISCAQISLVEIKTGFKLRSAVMGDNQCITVLSVFPLETDADEQEQSAEDNAA"
  },
  {
    "reference": "MVGDIATRWKELSGSSKWKDLLDPLDLDLRRYILHYGDMAEVGYLAFNSDRRSKYVGDSCYTKEELFARTGYLKANPFRYEVTKYIYGTSSIRLPECFIIKSLSREAWNKESNWLGYIAVATDEGKKLLGRRGIVVAWRGTIQLYEWANDFDFPLESAVMVFPGANPNDEPRVANGWLSLYTSTDPRSRFDKTSAQEQVQEELKRLLELYKNEDVTITLTGHSLGAVMSILSAADFLHNEWPKITPSLQHSLCVTVFAFGSPQIGDRSFKRLVESLEHLHILRVTNVPDLIPRYPVFRFTDIGEELQINTLKSEYLKRSLNLGHFHNLEAYLHGVAGTQHNQGEFKLEINRDIALVNKGLDALEDKYLVPGHWWVLENKGMVQSDDGTWKLNGDRSKKKQEEEDEKEENNCKFP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The Acyl-ester intermediate active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "VEIGIVGKYVELQDAYKSIEESLLQAAVYNGRRIKIRLIHSEKITPSNVGEMLAGLDGVIIAPGFGQRGIEGKFTAIRWCRETDIPTLGICLGMQCMVIEFARNVLGLEGANSTEMDPGTPYNVIDLMEEQKSITNLGGTMRLGAYRCSLLPGSLAAKAYGKTDVTERHRHRFEFNSDYRARFEAAGMKCVGENPETHLVEVVEIPSRRWMIGTQYHPEYSSTVLSPSPLFISFVRKSTQ",
    "response#2": "EDCLYLNIYAPAHAETGSKLPVMVWFPGGAFETGSASIFDGSALASYENVLVVTIQYRLGIFGFFNTGDEHARGNWAFMDQVAALVWVQENIEFFGGDPRCVTIFGESAGAISVSSLILSPMTKGLFHKAIMASGVAIIPYLKASDYERNDDLQTIASICDCNASDSVALLQCLRAKSSEELLSISQKTKSFTRVVDGLFFPNELLDLLAQKLFHLVPSIIGVNNHECGFLLPMKEFPEILGGSNKSLALQLIHSVLHIPVQYSYLVADEYFHNKHSLLDIRNRFLDLLGDVFFVVPGLVTAQYHTDAGAPVYFYEFQHRPQCLKDRKPPFVKADHTDEIRFVFGGAFLKGNIVMFEEATEEEKALSRKMMRYWANFARTCS",
    "response#3": "VEIGIVGKYVELQDAYKSIEESLLQAAVYNGRRIKIRLIHSEKITPSNVGEMLAGLDGVIIAPGFGQRGIEGKFTAIRWCRETDIPTLGICLGMQCMVIEFARNVLGLEGANSTEMDPGTPYNVIDLMEEQKSITNLGGTMRLGAYRCSLLPGSLAAKAYGKTDVTERHRHRFEFNSDYRARFEAAGMKCVGENPETHLVEVVEIPSRRWMIGTQYHPEYSSTVLSPSPLFISFIKQH"
  },
  {
    "reference": "MSRFIFITGGVVSSLGKGIAAASLAALLEARGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVRTTMGKNNNFTTGQIYESVIRKERRGDYLGGTVQVIPHITNEIKESVLLGAGDADVCLVEIGGTVGDIESLPFLEAIRQMGVELGHQHALFMHLTLVPYIKVSGEIKTKPTQHSVKELRSIGIQPDILVCRSEKPLPEGERKKIALFTNVAEEAVFSAVDADSIYRIPLMLHAQNLDEVAVKLLGLDLPAADLSEWQGYIDGYDALHDEVHVAMVGKYVHLTEAYKSLSEALTHAGVHTGSKVKIHYIDSEKLEQDVGDQLRGMDAILVPGGFGERGVEGKIAAVRFAREQGIPYLGICLGMQVAVIEYARNVAGLTDAHSTEFNPDSPNPVIALITEWLNADGKREQRDENSDLGGTMRLGGQECRLEPDSKVRELYGEEVIVERHRHRYEFNNSYLDRIVTAGMKIAGRSIDGRLMEIVEIPDHPWFIACQFHPEFTSRPRYGHPLFSGFIEAARKHRDATA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit glutaminase activity, CTP synthase activity.2. The protein with the reaction H2O + L-glutamine = L-glutamate + NH4(+), the active site must be optimized to ensure efficient catalysis.3. The designed protein should interact with enzymes and substrates within the CTP from UDP: step 2/2 pathway to enhance or regulate its activity.4. The designed protein should have a binding site that is specific to the target ATP ligand. The designed protein sequence is ",
    "response#1": "MPDAYLSMMEALLHAGIANDARVNIKWVNTEALEEGGQIEEQLGDVDGILVPGGFGVRGIEGKIKAAEYARTNGVPYLGICLGMQVAVLEYARNVVGLAEANSTEFDPYAPYKVIDLMPEQLETADLGGTMRLGDWPMDLAGGTKLAQLYGVPQGGEVQERHRHRYEVNPVYVQQLKDAGLCISGVTPGMAGRGAGLVESIELPDHPFFVGLQAHPEFKSRPMRPSPPFAGFIEA",
    "response#2": "MPDAYLSMMEALLHAGIANDARVNIKWVNTEALEEGGQIEEQLGDVDGILVPGGFGVRGIEGKIKAAEYARTNGVPYLGICLGMQVAVLEYARNVVGLAEANSTEFDPYAPYKVIDLMPEQLETADLGGTMRLGDWPMDLAGGTKLAQLYGVPQGGEVQERHRHRYEVNPVYVQQLKDAGLCISGVTPGMAGRGAGLVESIELPDHPFFVGLQAHPEFKSRPMRPSPPFAGFITAR",
    "response#3": "MPDAYLSMMEALLHAGIANDARVNIKWVNTEALEEGGQIEEQLGDVDGILVPGGFGVRGIEGKIKAAEYARTNGVPYLGICLGMQVAVLEYARNVVGLAEANSTEFDPYAPYKVIDLMPEQLETADLGGTMRLGDWPMDLAGGTKLAQLYGVPQGGEVQERHRHRYEVNPVYVQQLKDAGLCISGVTPGMAGRGAGLVESIELPDHPFFVGLQAHPEFKSRPMRPSPPFAGFIETRG"
  },
  {
    "reference": "MAKEKFDRSKPHLNVGTIGHVDHGKTTLTAAITTTLAKAIGGKNKAVAYDQIDNAPEEKARGITIATSHQEYETANRHYAHVDCPGHADYVKNMITGAAQMDAAILVVSATDGPMPQTKEHILLARQVGVPYVIVFINKADMLAADERAEMIEMVEMDVRELLNKYSFPGDTTPIVHGSAVKALEGDESEIGMPAILKLMEALDTFVPNPKRVIDKPFLMPVEDVFSITGRGTVATGRVEQGVLKVNDEVEIIGIRPTTKTVVTGIEMFRKLLDQAEAGDNIGALLRGTKKEEIERGQVLAKPGSITPHKKFAAEVYVLTKDEGGRHTPFINNYRPQFYFRTTDVTGVCNLPNGVEMVMPGDNVSLTVELISPIAMDKGLKFAIREGGRTIGSGVVAEITE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design must be able to perform GTP binding, GTPase activity in order to contribute to translational elongation. The designed protein sequence is ",
    "response#1": "PVVAIVGRPNVGKSTLFNRLAGGLVAIVENRPGVTRDRLYRDSEWLGRKFTIIDTGGIEFVNENTPITAQMRRQAEIAIEEADVIVFVIDAQISPTPDDDMIAQTLRRSGKPVILAANKVENFAKTELYEFYNLGLGEPVPISAVHGMNIGDLLDEVVSHFPEDR",
    "response#2": "IHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSSEPPYSEARYEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTNMPWFKGWKVERKEGNADGKTLIDALDAILSDIRNIGILAHIDAGKTTTTERMLFYAGKTRSLGEVHRGNTVTDYLTQERERGITICSSAVTFPWNGKRINLLDTPGHIDFTMEVEQSLHAVDGVIVVLDATAGVEAQTMTVWSQADKHRLPRLVFVNKMDRPDADFNKCVEDLKSKLETFPVCIQYPAKSNEGQLGIYDVITLEQLNWQQKDLGRSYSKLKLEPSDGLRQLQDKRNELIDQLSGLDDELADVVISTESFDKVSNELVNKALRRVICQQKAVPVLLGSAYKNIGIQCLMDAVNHYLPAPFTAQVIVLNHPGQIANGYTPVLDCHTAHIACKFAEIKEKVDRRSGKTTEENPKFIKSGDAAIVNLVPSKPLCVEAFQEFPPLGRFAVRDMRQTVAVGVIY",
    "response#3": "RNLGILAHVDAGKTTVTERILYLTGTTHKRGEVHDGTTVTDFDPQERDRGITIFAAAVSCAWAGHRINLIDTPGHVDFADEVERSLRVLDGAVAVFDAVAGVEPQSESVWRQADRHGVPRIAFVNKMDRAGADLDAAVASIRERLHPVPLVVQLPIGTEDGFTGVVDKI"
  },
  {
    "reference": "MRLVLASNNAKKLKELGTLLAPAGVELVTQGSLGIAEAEEPHHTFIENALAKARHAAAASGLPAIADDSGLCVDALGGQPGVQSAHYATLDPADIDGLAREALRERQDAANNRRLLSALDGQANRRARFVCTLVAIRSADDPEPLVALGRWEGELLTGLRGSGGFGYDPLLSIPALDATVAQLDAETKNRHSHRALAAEQMVALMRSAWHLA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a high affinity for Mg(2+).2. The designed protein must be able to perform dITP diphosphatase activity, nucleotide binding, ribonucleoside triphosphate phosphatase activity under specific conditions.3. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.4. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..5. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "MENTWKD",
    "response#2": "MTERR",
    "response#3": "MSILSGIKRVF"
  },
  {
    "reference": "MASSTPAAPGAAAHALPSVLAPAPGHHQSQSQSQSQHQHPLHHQQGPSASPLHPPGAYYPPPPPRSTVSSVLSASPPPAPVLTAPELHPAGGRVYDPTTDTTKERPIAASRQHAPQVATPKVSRHKHSNSSPPPYQPREPFQYPQPIDQQHQPSSYRNGDNNYPSPRERASSFTRRPTSPLSLSHSHPSLATGSHSPPSRHSHMTSPVSRHAPAPTHATNGTSLAPIMKTDLAAPSPPKPASSRAADPMSFSNILSNSEPAPEPVTREPSPKKEPTLAAPIFELREPDNDGDTEMEKEADTEPEPAPVKKSETVKKKKPAKKPAKGRASEIRGEDASKKNRRPSIKKESPTPRPPSKRQANGQPKQKTWSSEMERSIQHHEALLDKQAESLDEDEFSERAYKEGAFKRQRIMAQLDADKAYARREQFANTTGKRLAEHSDLGKRRYDDVFYDEALQEVREQELFAEKERKKDMQRKRRREKSMAVTIEQKEAALARAEAAEDEEERQKHLRDAERANKKAQQTKLILQKGIKGPARNVELNLDGGTMSSFLASDAETPKKSKGRGGTRPKKSKEQKQAEKDSAEAAQAALDAGEELPPKEEGRVRIKIKGKSKGGDGEKAAKEKAEAAKEKEKVDDVPDLEKKFISKGYNQIYDQIWRDMARKDVSKTFKLAVDSYATRGSNLKKTAILASKEAKRWQLRTNKGTKDLQARAKRVMRDMMSFWKRNEREERDLRKAAEKQEIENARKEEADREAARQKRKLNFLISQT",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.2. The ideal protein would be one that plays a critical role in telomere maintenance via recombination through DNA binding, 3'-5' DNA helicase activity within Ino80 complex.3. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.4. The designed protein should contain one or more Helicase ATP-binding domains that are essential for its function. The designed protein sequence is ",
    "response#1": "YPVMEKSLEFWRARDFARKLEVLKPLFVEPRSKGGFSEVWSLGSTALLGWPLSLTRGSGQLQAVWHLVPAGTASPQRRIPAALLVEVEGPRAGARLTSQSFQVMDAYYARVAAPGSSGATFLAVCRGKASEGLDFADVNGRGVIVTGLPYPPRMDPRVVLKMQFG",
    "response#2": "YPVMEKSLEFWRARDFARKLEVLKPLFVEPRSKGGFSEVWSLGSTALLGWPLSLTRGSGQLQAVWHLVPAGTASPQRRIPAALLVEVEGPRAGARLTSQSFQVMDAYYARVAAPGSSGATFLAVCRGKASEGLDFADVNGRGVIVTGLPYPPRMDPRVVLKMQFR",
    "response#3": "YPVMEKSLEFWRARDFARKLEVLKPLFVEPRSKGGFSEVWSLGSTALLGWPLSLTRGSGQLQAVWHLVPAGTASPQRRIPAALLVEVEGPRAGARLTSQSFQVMDAYYARVAAPGSSGATFLAVCRGKASEGLDFADVNGRGVIVTGLPYPPRMDPRVVLKMQFR"
  },
  {
    "reference": "MSLSVVILAAGKGTRMRSALPKVLHPIANKAMVNHVVESARALNADNIYLVYGFGGDVLQQKVIGDDLTFVEQVQQLGTGHAVDQASPFLKDDEDVLVLYGDVPLTKISTLESLLAVKPEEGMALLTVFLNNPTGYGRIIRKQINGQSQVVGIIEQKDATAEQLKVNEANTGILLANGGDLKRWLSGLSNDNAQGEYYLTDIIAACHAEGKTIATAHPDAEIEVEGANNRVQLAALERAYQYRQAEELMLAGASLYDPSRIDIRGELNISEEVVIDINCIFEGKVNLAKGVKIGANCILKDCFIGENTEIKANTIIESSTIAENCSVGPFARLRPNSVMQAGSHVGNFVEMKKTTLGEGAKAGHLSYLGDSEIGAKVNIGAGTITCNYDGQNKFKTIIGDNAFIGSNSSLVAPVTIGKKATIGAGSVIAKDVAENELAVARGKQRNIQGWQRPKKLVK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be able to bind Mg(2+), acetyl-CoA ligand in a variety of conditions2. The designed protein must possess magnesium ion binding to support regulation of cell shape, lipid A biosynthetic process, UDP-N-acetylglucosamine biosynthetic process under certain conditions.3. The desired protein should be able to perform acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate4. The presence of Mg(2+) is necessary for the protein to function in the desired environment.5. A protein that is capable of catalyzing reactions within the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway with high efficiency and specificity.6. The designed protein should contain one or more MobA-like NTP transferase domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MNAILAAGKGTRLKSKYPKVLHRVGGKPLLSHVIAAATRIVPASEIYVVLGHEAKQVEQAIADSGVQFIEQAEQRGTGHALQVARPMLEKFDTVVVLSGDAPLITSETIQSLLKFHRDKHAAMSLLSAHLPHPHGYGRILRAENPSGYGRLIERDGNVTAIVEQKDATPEQLAIHEVNTSVYVFDAATLRGALDALSQDNVQGEFYLTDVIALARNDGGRVSAHICDDPMLIEGVNDRVQLARLGAEMNRRICDAHMRAGVTIMQPESVRIDPDVEIGPDTILEPGVMLLGSTRVGSDCSIGAYSRITNSRIEDGVTILQCSVVADSIIKREASVGPFAHIRPGTEVGPQAKIGNFVEVKKSKIGRGTKAGHLTYLGDAIIGDNVNVGAGTITCNYDGEKKHTTIIESNAFIGSGTELVAPIRIGRNAYVGAGSTITKNVPADALAVARTHQRNIEGWVARKRPGTPAAEAANKALAERLAEEGAP",
    "response#2": "MTILGAGLGTRFKSKRAKLLHEAGGRKLIEHAVRTAAKLSASKIFAIVGYQADAIQKAITEADSKLAGGKVRFLLQDKPLGTGHALKCGQKELRAAAPLLLVYCGDTPLLRPETLRDFLSYHRRLKATATVMTAIMADPSGYGRMVRSADGQVDAIVEHKSASADILAIREINTGIYCFDTKELFSQLDRLKPDLAAGQISEYYLTVAADQLATIGKNASVGPYARLRPGTSLGDGARIGNFVESSGNVLGINTRSDLAEARRILHRRVIERHMLDGVTIMQPESVRIDPDVEIGPDTILEPGVMLLGSTRVGSDCSIGAYSRITNSRIEDGVTILQCSVVADSIIKREASVGPFAHIRPGTEVGPQAKIGNFVEVKKSKIGRGTKAGHLTYLGDAIIGDNVNVGAGTITCNYDGEKKHTTIIESNAFIGSGTELVAPIRIGRNAYVGAGSTITKNVPADALAVARTHQKTIQGWVKRRRPGTASEDAARAAQQSSGGGR",
    "response#3": "VVVLAAGGGTRMKSTKSKLLHEIAGKSMLSFAVSAAAALDPGHLVVVVGHQREQVEEHLEKLSEAVSTAVQEQQLGTGHAVACGLAALGDLDGEVVVTYADVPMLTGDTLRDLVATHRGNLNAVTVLTAVVDDPTGYGRIVRDGGNVTGIVEHKDATEAQLQINEINSGIYVFDAATLRAGLASLTTDNSQGEQYLTDVVHFAAQRSGRVGAHRIDDVWSVPDADEVLGCNDRIQLAEARRVLNDRVLRRHMLAGVTVVDPASTWIDVDVAIGQDVTIEPGTQLRGRTSIGPDAVVGPDTTLTDVVVGEGAQVIRTHGSDAVIGAGATVGPFAYLRPGTELGANGRIGTFVETKNAQIGAGSKIPHLTYVGDATIGEQSNIGASSVFVNYDGVNKHRTVIGSHCRTGSDNMFVAPVTVGDGAYTGAGTVVRQNVPPGALAVSAGEATIGKNASVGPYARLRPGTSLGDGARIGNFVGRDGVTVVDPASTWIDVDVAIGQDVTIEPGTQLRGRTSIGPDAVVGPDTTLTDVVVGEGAQVIRTHGSDAVIGAGATVGPFAYLRPGTELGANGRIGTFVETKNAQIGAGSKIPHLTYVGDATIGEQSNIGASSVFVNYDGVNKHRTVIGSHCRTGSDNMFVAPVTVGDGAYTGAGTVVRQNVPPGALAVSAGEAVPVGADTVIGR"
  },
  {
    "reference": "MENRWQVMIVWQVDRMRIRAWHSLVKYHMYRSRKAKDWFYRHHYESRHPKTSSEVHIPLGDARLVVRTYWGLQTGEKDWHLGHGVSIEWRQKRYSTQIDPDLSDKLIHLYYFDCFSESAIRKAILGQVVSPRCEYQAGHNKVGSLQYLALRALATPTKVKPPLPSVKKLVEDRWNEPQKTRGRRGSRSMSGH",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that In turn, they are directed to the 26S proteasome for degradation; Finally, Vif also contributes to the G2 cell cycle arrest observed in HIV infected cells.; Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions2. The designed protein should contain at least one HCCH motif motif to achieve its intended function.3. The protein should exhibit Basic and acidic residues compositional bias.4. The designed protein localizes to the plasma membrane. The designed protein sequence is ",
    "response#1": "DPPTF",
    "response#2": "EHHW",
    "response#3": "MEPVDPNLEPWNHPGSQPKTACNNCYCKICS"
  },
  {
    "reference": "MMTLEDLIGLLDPLEYLAGRTPLKEVHALTADSRQAVEGGAYFALRGYVTDGHHFISDALSKGVGVVVCEEIPVSVDERCCYIRVADCRTALAAAAAEWFGHPASSLRVIGVTGTNGKTTTARLIRTMLDRSGVKTGYIGTGQALIGDESIVLERTTPEAMDLHALFRKMVNCGCEAVVMEVSSHALVLHRTEGISFYGAVFTNLTPEHLDFHATMDEYGSAKQILFRQVAPDGFGIVNADDAWGSMMAGAFSGSRLFCCTVGSQVFGCTNGSVVRARDIEARMDGSSVTVQMNGRACRASFLLPGLFNVMNMLEILSAGVAMGLSLETAAASVADVVSVEGRMEPVSDDGGLFSAIVDYAHTPDALEKVIGALNEVRPADSSLIVVFGCGGDRDRSKRPLMGGIAAAGADHVILTSDNPRSEDPEEILDAIASGITEGSFMRIADRADAIVCGVQMLRPGDLLLIAGKGHECYQESGGRKSYFSDREVVARALQERAD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be able to modulate cell division, cell cycle, cell wall organization in a way that leads to a desirable outcome.2. The protein should be optimized for activity in Cell wall biogenesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "GILTDGKGRIGSIVANRQFQFDGPPPQAGAIYAAGWSITPDGNLAIGDNDVFYQCLSGTFYNLYDEHIGSQCTPC",
    "response#2": "GILTDGKGRIGSIVANRQFQFDGPPPQAGAIYAAGWSITPDGNLAIGDNDVFYQCLSGTFYNLYDEHIGSQCTPL",
    "response#3": "GILTDGKGRIGSIVANRQFQFDGPPPQAGAIYAAGWSITPDGNLAIGDNDVFYQCLSGTFYNLYDEHIGSQCTPS"
  },
  {
    "reference": "MTSEQRRNLLTFQDYVRKILDPTHILCYMAPWFREDKMQYIQAEKNNRGPMEAASLFLQFLLELQEEGWFRGFLDALNQAGYSGLYEAIENWDFNKIEKLEVYRTLLKRLQPEFKNRIIPLEILPELAECLISQECEEIRQTCYNKGMIAGSEKLIECLLKSDKENWPKVLKLALEKEQNNLSEMWLVEKGEKDIENKDLEDDEMETSEIHIFFQEDPEHQNLSQNSQAPSEMSRTNLKPRNYQLELALPAKEGKNTIICAPTGCGKTLVSLLICEHHLKNFPQGRKGKVAFFANHVPVYEQQKSVFLSYFERLGYSVAGISGATTENVPVEQTIKNNDIIILTPQIIVNNLKNGTIPSLSVFTLMIFDECHNTNKHHPYNMIMFNYLDQKLGGSSDPLPQVVGLTASVGVGDAKTTDEAMLYICKLCASLDATVIATVRDNLEELEQVVYKPQKFSRKVESRTTNKFKFIVSQLMRDIESLARNICAEFGNLFQTQNGDFGTQKYEQRIVGVHKAGMVFRLPDKEEESRICKALFLYTSYLRKYNDSLIISEDAQMKDALNYLKDFFNNVRTAGFDETEQDLTWRFEEKLEELESVCRDPSNENPKLNDLCLILQEEYHLYPETRTILFVKTRALVDALKKWIKENAALNFLKPGVLTGRGRTNQNTGMTLPAQKCALDAFREDKDNILIVTSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCYLLTSNADVIEKENINMYREEMMNDSISKLQTWD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.2. The designed protein must have at least one RLR CTR, Helicase ATP-binding, Helicase C-terminal domain.3. A protein that contributes to positive regulation of myeloid dendritic cell cytokine production, cellular response to exogenous dsRNA, RIG-I signaling pathway, positive regulation of tumor necrosis factor production, positive regulation of granulocyte macrophage colony-stimulating factor production by functioning in ruffle membrane, actin cytoskeleton, ribonucleoprotein complex, cytoplasm, bicellular tight junction with identical protein binding, double-stranded DNA binding, zinc ion binding, single-stranded RNA binding, RNA helicase activity.4. The protein should be designed to have high stability and activity towards ATP + H2O = ADP + H(+) + phosphate. The designed protein sequence is ",
    "response#1": "DQRSEFQADTVRLLCTNCAEPVGYGSDLRKVEGTHHVNVNPNFIFYYKTSLKPVTIDRTFKDWQPGGAISCKKCGEAWGLEMIYKSVKLPVLKIRSLVLETPQGRRKVNQWSKVPFQVADLDYLRDCPEETKKARSYQIELAQPAINGKNALICAPTGSGKTFVSILICEHHFQNMPAGRKAKVVFLATKVPVYEQQKNVFKHHFERQGYSVQGISGENFSNVSVEKVIEDSDIIVVTPQILVNSFEDGTLTSLSIFTLMIFDECHNTTGNHPYNVLMTRYLEQKFNSASQLPQILGLTASVGVGNAKNIEETIEHICSLCSYLDIQAISTVRENIQEKLTKLRNTILEQYTRTEESARGIIFTKTRQGAYALSQWISENEKFAEVGVKAHHLIGAGHSSEFKPMTQNEQKEVISKFRTGKINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQGLRQQRGWALLDEEYRLNPETRTILFVKTRALVAALKKWIDENPALSYLKPDTLMGRSKRNEQTGMTLSNQKGVLDSFRSNGDSKILIATAVADEGIDIAQCNLVLLYEYSGNVTKMIQVRGRGKLEDLCFVLQEEYHFNQETKTILFVKTRALVDALKKWIEENSNLSFLKPGILTGHGKTNQNIRMTLPAQKCILDSFRASGDNNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRAKGSKCFLLTSNNDVIEKEKINICKEKMMNDSIL",
    "response#2": "IMPALEGKNVIIWLPTGAGKTRAAAYVAKRHLETVDGAKVVVLVNRVHLVTQHGEEFSRMLGGRWTMTTLSGDMGPRAGFGHVVRSHHLLICTAELLQMALTSSDVEEHVELTAFSLIVVDECHHTHKETVYNVILNRYLEHKLQRARPLPQILGLTASPGTGGASKLDGAINHILQSQRQQFSAEHVQLLCINCMVAVGHGSDLRKVEGTHHVNVNPNFSIYYNVSREPVVIDRVFKDWRPGGTISCRNCGEVWGLQMIYKSVNLPALRIRSVLLETPRGRIQAKKWSRVPFPVADFNILEDSL",
    "response#3": "LEDHRSLLRRLEPEFKARIIPTEILPEIAECLITQDCEEIRQVCISRGPMAGAEKMAECLLRSDKENWPKILKLALEKDGNIFNELWAQPAINGKNALICAPTGSGKTFVSILICEHHFQNMPAGRKAKVVFLATKVPVYEQQKNVFKHHFERQGYSVQGISGENFSNVSVEKVIEDSDIIVVTPQILVNSFEDGTLTSLSIFTLMIFDECHNTTGNHPYNVLMTRYLEQKFNSASQLPQILGLTASVGVGNAKNIEETIEHICSLEEYRLLLKRLQPEFKTRINPEDILPEISECLIKQECEEIVQVCSNKGTMAGAEKMVECLLRSDKENWPKTLTFALEKEESRFSELWITPVKPRNYQLELALPAKEGKNTIICAPTGCGKTFVSLLICEHHLKNFPQGQKGKVVFFAVQLPVYEQQKSVFSNYFERLGYKVAGVSGATSENVSVKQIVENNDIIILTPQILVNSLKNGTIPSLSVFTLMIFDECHNTGKHHPYNMIMFNYLDQKLGGSSDPLPQVIGLTASVGIGDAKSTAEAVEYICRLCASLDTSVIATVKDNLEELEEIMPALEGKNVIIWLPTGAGKTRAAAYVAKRHLETVDGAKVVVLVNRVHLVTQHGEEFSRMLGGRWTMTTLSGDMGPRAGFGHVVRSHHLLICTAELLQMALTSSDVEEHVELTAFSLIVVDECHHTHKETVYNVILNRYLEHKLQRARPLPQILGLTASPGTGGASKLDGAINHILQKQYDLCHRHTQDPFGDMLKKLMDQIHDHLEMPKLRRDFGTQTYEQQVVELSQDAAEAGLLEQRVYALHLRRYNDALLIHDTVRAVDALDTLRDFYNRERATKTGVLHAERWLLALFDDHKNELARLA"
  },
  {
    "reference": "MADKQMSVVILAAGKGTRMYSDLPKVLHSLAGKPMVQHVIDAAKGLNAKNIHLVYGHGGELLKTTLNDPALHWVEQAEQLGTGHAMQQAAPWFAQDEDILMLYGDVPLISQQTLQRLREQKPEGGIGLLTVVLENPFGYGRIVRENGEITGIIEQKDASPEQLNITEINTGILVADSSDLQRWLAKLSNNNAQREYYITDIIAMAHQEGRRIVAVHPQNSSETEGVNNRQQLAALERIYQRETANKLLLAGVMLNDPTRFDLRGTLQHGRDVTIDINVILEGQVILGNGVTLGAGCVIKNSVIGDGCEISPYSVIEDATLEADCTVGPFARLRPGTSLAEQVHVGNFVEVKKAMLGKGTKAGHLSYLGDAEIGANVNIGAGTITCNYDGLNKSKTIIGDGVFVGSDSQFIAPVTVADGVTIAAGTTVMKDVNEESLVYNPKQQTEKTGWSRPAKK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.2. A protein with glucosamine-1-phosphate N-acetyltransferase activity, magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity functions is required to enable lipid A biosynthetic process, peptidoglycan biosynthetic process, cell morphogenesis.3. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.4. The UDP-N-acetyl-alpha-D-glucosamine binding site should be stable and able to withstand changes in environmental conditions.5. We require a protein that can specifically bind to the metabolites produced in the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, Nucleotide-sugar biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVEVPDATGYGRIVRDEAGDVSAIVEHKDASEAQRAIREINSGIMVLPTARLKDWLGRVGNDNAQGEYYLTDVIAMAAADGLPVGAHQPRDLLQTEGANDRAQLATLERAHQRRVAERLLEQGVHLIDPARFDLRGHLTIGKDVRIDINVIIEGECELGDNVEIGAGCIIKNTKISANTKVQPYSVFDNAFVGESVQIGPFARLRPGAKLANEVHIGNFVEVKNSSIGFASKANHFAYLGDAQIGSGSNIGAGTITCNYDGANKHKTVIGDDVFIGTNNSLVAPVKIGNGATTGAGSTITRDVADNALAVERSQQRNIDGWKRPVKQP",
    "response#2": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLTVELDDPTGYGRILREGDRVRAIVEHKDATPEQRAIREINTGILVAPTTALKNWLARLDNNNAQGEYYLTDVIAMAVADGVAVRTAQPERLSEVAGVNNRLQLAELERAWQLRQAEALLLGADVTIGRDTLVEPGCVITGATVIGEGCHIKPHCTIEGVTLIDAEHTYIGPDVTIGADTVIYPGTVLRGRTVIGEDCSIGPATEMSDTIVGSGATIRQTVTDGAEIGDQCNVGPFAYLRPGTKLGRNVKVGDFVEIKNSVIGEQSKVPHLSYIGDAVVGGNVNIGCGAITANYDGYNKFKTEIGDNVFVGSNANLIAPVKIGSGAYIVAGSTITHNVEENDLAIARERQRNIEGWVRPQRK",
    "response#3": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLTVELDDPTGYGRIIRNDDGSVRRIVEEKDATSAERAIREINSGVYVFRASVLRDALGRLRTSNDQGEKYLTDVIGLVRAEGGSVGAFRTHDVWQTEGANDRVQLATLGAELNRRIVARWQRAGVWMLDPGRVYIDVSVRLEAGVRILPGVHLEGSTSVAEGAELGPDCFIRDSRIGPGARIWYSVVRGVSVGTDAQVGPYASLRPGVELGDSAKAGTFVEVKNSVVGARAKVPHLAYVGDTDIGEEANVGAGTITANYDGYNKHRTRIGSRAQIGSNTVLVAPVEVGAEAYTGAGSAITRDVPDGALGVERTSQRNIEGWVERKRPGSAAAEAAAAARGGTPDTAEET"
  },
  {
    "reference": "MVKNLVLWLIIAVILMSVFSNFGPRHAQVTKIPYSQFVDQVKQGDVKSVTIDNRTVRGATRNDDNFVTYMPLQDDYILPELIKNKVVVNGKAPEQQSLLMSIFISWFPIILFIGVWMYFMRQMQGGGGRGAMTFGKSRARLLGEDQVKVTFADVAGVDEAKEEVKELVEFLKDPEKFQRLGGRIPRGVLMVGQPGTGKTLLAKAIAGEAKAPFFTISGSDFVEMFVGVGASRVRDMFDQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFEGNEGVIVIAATNRPDVLDKALLRPGRFDRQVVVGLPDVRGREQILKVHMRKVPISDNVEPRVIARGTPGFSGADLANLVNEAALSAARFNKRLVDMEEMERAKDKIIMGVERHSMVMNAQERRLTAYHEAGHAIVGLTVPEHDPVYKVTIIPRAQALGVTMFLPEEDRYSYSKKRLEGQITSLFGGRIAEELIFGADSVTTGASNDIKRATEIARNMVTKWGLSERLGPLTYNEDEGEVFLGHSMNTRKEVASTTASVIDEEIRKIIDRNYERAKEILVEKQAILHSMAEALIKYETIDVGQLEDLMAGRPPREPRDWTTSGSSSEPPSATPVTNDTAASKDESSTTEKRNLDDSDTAKSH",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.2. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins; Plays a role in the quality control of integral membrane proteins.3. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.4. The presence of Zn(2+) is necessary for the protein to function in the desired environment.5. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.6. A protein with ATP hydrolysis activity, ATP binding, zinc ion binding, ATP-dependent peptidase activity functions is required to enable protein catabolic process, proteolysis. The designed protein sequence is ",
    "response#1": "IPRGVLLVGPPGTGKTMLAKAVAGEAGVPFFGLSGSDFVEMYVGVGAARVRDMFQQALQRSPAIIFIDELDALGKVRGSGAPGGHDEREQTLNALLVEMDGFGTDHSVIVMGATNRPETLDPALMRPGRFDRHVLVDRPGGLGDILEVHDKSEDAKKIISEIVDLKRVAKLTPGFVGADLANLVNEAALLSARREDKRVTMKSFENAIIPRGVLLVGPPGTGKTMLAKAVAGEAGVPFFGLSGSDFVEMYVGVGAARVRDMFQQALQRSPAIIFIDELDALGKVRGSGAPGGHDEREQTLNALLVEMDGFGTDHSVIVMGATNRPETLDPALMRPGRFDRHVLVDRPEDLENVDLKVVAKGTPGLVGADLANLVNEASLLAARNGKSKVSMEDFEEALKVIRTGKD",
    "response#2": "IPKGALLVGPPGTGKTLLAKAVAGEAGVPFFSMSGSDFVEMFVGVGASRVRDAFRLAKEKAPCIIFIDEIDAVGRARSKNPAMGGNDERENTLNALLTEMDGFGTNSGVIVLAATNRADMLDKALLRAGRFDRQINVDLPGLEERERLIKLHLDKYNIQAPEGDVDLDIIAAAAVGLTGADIRNIVNEAALWAARNDKSVVSMADFIRAVKAKQKGSKMILSDEEKELTSYHESGHALMAKLLPDADPVHKVTIIPRGRALGLTAQLPIDEKHNYSQSYIKTRLLVLLGGRAAEKLVFNELTTGAGNDIEIATELARKMVCEWGMSSELGPLTYGKKDEEIFLGREIATHRDYSEMTAQKIDKEVARIVWEVENESLALLKKNLDKLHNLARELLKHETIDGADMNKIVDDLLKLD",
    "response#3": "MVMTEDEKKLTAYHEGGHAICALHMVDSDPIHKATIIPRGRALGMVMRLPEGDRISMSRAKLEADLVVAMGGRVAEELIFGEEKVTTGASSDIKMATNMARKMVTEWGMSNKLGPLLYAEDQEEVFLGHSVARHKNLSDETARLVDEEIRRIVDTAYGKARDILSTRQDELERLAQGLLEYETLSGDEIREYYARIRE"
  },
  {
    "reference": "MKDDFVIYNETDFYIKPYQDDFNAIFQKIKTLLAITEPLELSLIIVDAQEQLELNQKYRHKDYVADVITFALEEENKIDLFELTGLRSLGDIFICYEKALAQAAEYNHSPRREFAFLFTHGMLHILGYDHQTPADEEKMFNLQRKVLNDLQINRIPIK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have metalloendopeptidase activity to facilitate rRNA processing. The designed protein sequence is ",
    "response#1": "SQVGCAVNCAFCASAKNGFVRNLSVGEMVVQLLAFRKYVTPDLHSVVIMGTGEPLLNYDNVLAFLRLIHEKDTFYLGYRNMALSTSGIVPAVDRLAAEGLPLTLAISLHGPTNAIRRQIMPLTDTYDVPAVIAAGDRYFAATGRKVTYEYILIKNE",
    "response#2": "SQVGCAVNCAFCASAKNGFVRNLSVGEMVVQLLAFRKYVTPDLHSVVIMGTGEPLLNYDNVLAFLRLIHEKDTFYLGYRNMALSTSGIVPAVDRLAAEGLPLTLAISLHGPTNAIRRQIMPLTDTYDVPAVIAAGDRYFAATGRKVTYEYILIKNN",
    "response#3": "SQVGCAVNCAFCASAKNGFVRNLSVGEMVVQLLAFRKYVTPDLHSVVIMGTGEPLLNYDNVLAFLRLIHEKDTFYLGYRNMALSTSGIVPAVDRLAAEGLPLTLAISLHGPTNAIRRQIMPLTDTYDVPAVIAAGDRYFAATGRKVTYEYILIKNE"
  },
  {
    "reference": "MRRTCCLFNSLDSTTRIVPNAIRINHTNSMSLRTSTVSTSTKMFTKHAKNRIGNSIRLRDELYQNYLKNYKKIQLYKKSQPPKKIEYEFVLDILFYGSCAIVFYAICEKIHDRKIRRSLVKEDFSDYPYHGL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The designed protein localizes to the membrane. The designed protein sequence is ",
    "response#1": "GRTANGKVRLYVIGQGALSAAVAANSKHDIAEAERWNLPIILIVLLAVFGSLAAAAVPLALGVCTVVVTMGLVDLVSMHTIMSVFVTSTVSMFGIALAVDYSLFILMRFREELRSGRQPQEAVDAAMATSGLAVVLSGMTVIASLTGIYLINTAALKSMATGAILAVAIAMLASITLTPAALATFGRAAVKRSVLMHWSQRSECTQSLFWTRWVGWVMHRR",
    "response#2": "NCSALLGAVLSVDPEDLGARETVDWMRTELPKVPGAAHVNVGGPTALINDFDDRVAKTEPLMLVFVALIAFVMLLISIRSVFLAFKGVLMTLLSVAAAYGSLVMVFQWGWLENLGFTHINSIDSTVPPLVLAMTFGLSMDYEIFLLTRIRERFLQTGHTRDAVAYGVSTSARTITSAALIMIAVFVGFAFAGMPLVAEIGVACAVAIAVDVTAVRLVLVPTLMAMFAQWNWWLL",
    "response#3": "GRTANGKVRLYVIGQGALSAAVAANSKHDIAEAERWNLPIILIVLLAVFGSLAAAAVPLALGVCTVVVTMGLVDLVSMHTIMSVFVTSTVSMFGIALAVDYSLFILMRFREELRSGRQPQEAVDAAMATSGLAVVLSGMTVIASLTGIYLINTAALKSMATGAILAVAIAMLASITLTPAALATFGRAAVKRSVLMHWSQRSECTQSLFWTRWVGWVMHRR"
  },
  {
    "reference": "MRGPTGCGCAARWRWIAWPPSRAHSRHWWRNGSTVGRRTIRETVSIDGIGLHTGCRSTVTCRPAEPGHGVVFRRVDLPDMPTVPARLSEVSVTERRTGLGTGESAISTVEHLLAAVGALQLDDLLIDINAPEPPILDGSFEPYRDLLTRAQPEDHGGEAVIYRVTQPFQVTDADATYVVSPADTLRLTTIIEWDHPLIGRQVGSYDITAEAFAAELAPARTFGFVGEVDALRAKGLIKGASTENAIVLTDEGVEGTTLRWPDEFVRHKTGDIVGDLALLGGRLNAHIMATKPNHHGNIALARAITRTGQRTGGVAMNIAQILDVIPHRYPLLLVDRILEIEGTKRAVGIKNVTINEPFFQGHFPGHPIMPGVLIIEAMAQVGGMLLLEAMGDPEEKVVYFMSLDNVKFRRPVVPGDQLRCELEMIQFRGKTCRLRGVAYVDGAVAAEAEMMARIVDR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must have a active site, Proton donor that is structurally stable and resistant to denaturation.2. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation.3. A protein should be able to manipulate the activity of enzymes in the lipid IV(A) biosynthesis, Glycolipid biosynthesis, lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 2/6 pathway, either by activating or inhibiting their function.4. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.5. The designed protein should be involved in lipid A biosynthetic process, fatty acid biosynthetic process by localizing to cytoplasm and exhibiting 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity, UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity, 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity, (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity, UDP-3-O-acyl-N-acetylglucosamine deacetylase activity. The designed protein sequence is ",
    "response#1": "MGQSLAALGLKPLSVADAXXLASHXGXHXVPXTTXSXDXLLXXDXDXXQXGXPXVXCAPXKXRXEXSXDXGXDXDXDXDXGNXLXAAXSXAPX",
    "response#2": "MLQKLENILEGIILPKIEGNTKIVIDDSRKIKKGTLFFVLKGENFNGNKFIEEALKKGAEGALVEESEGLKPLEMPQIFVKNTRKALSLIANYFYKKPKEKIKVIA",
    "response#3": "MLRKLFNDVKYQILQGNLEKDIAXVXVXDDSRKVSEGFIFVAKGILLNEPHVEEAIKKGAKVVISELEFKVPDSIAVIKVNDVKRVNSLVAKEFYDYPSQKLKLVGITGTNGKTTTSYLIKEIFKEVGKKVGVIGTINYEIGNXIQKAHNTTPESVDLQKLFSKMHXEGVDYCIMEVSSHALELGRVYGCDFDVGVFTNLTQDHLDFHKTFEEYLNSKLKLFDMLKKDAIGIYNLDDKYSEKIIANSKCKQFTYSMKNDSXFYGKEFKLNEXGXSXFVEYKGXKYKFXLXIXGKFNVYNSLGAISCSXLLGIDXENISXALXKXPXIPXRLEXISXENFXVFVDYAHTPDAXEKVLKSXREXFKNXILXVFGCGGNRDKTKRPIMGKIALEXADXVIVTSDNPRSEXPEXIXKDXTKGXXNKQXVIXDRKXAIKFAIXKAKKGXVXLIAGKGHEXYQIIGXKXHHFDEXQVAKXFLXXI"
  },
  {
    "reference": "MNSSLTAQRRGSDAELGPWVMAARSKDAAPSQRDGLLPVKVEEDSPGSWEPNYPAASPDPETSRLHFRQLRYQEVAGPEEALSRLRELCRRWLRPELLSKEQILELLVLEQFLTILPEELQAWVREHCPESGEEAVAVVRALQRALDGTSSQGMVTFEDTAVSLTWEEWERLDPARRDFCRESAQKDSGSTVPPSLESRVENKELIPMQQILEEAEPQGQLQEAFQGKRPLFSKCGSTHEDRVEKQSGDPLPLKLENSPEAEGLNSISDVNKNGSIEGEDSKNNELQNSARCSNLVLCQHIPKAERPTDSEEHGNKCKQSFHMVTWHVLKPHKSDSGDSFHHSSLFETQRQLHEERPYKCGNCGKSFKQRSDLFRHQRIHTGEKPYGCQECGKSFSQSAALTKHQRTHTGEKPYTCLKCGERFRQNSHLNRHQSTHSRDKHFKCEECGETCHISNLFRHQRLHKGERPYKCEECEKSFKQRSDLFKHHRIHTGEKPYGCSVCGKRFNQSATLIKHQRIHTGEKPYKCLECGERFRQSTHLIRHQRIHQNKVLSAGRGGSRL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have at least one SCAN box, KRAB domain.2. The protein must exhibit the following characteristics: May be involved in transcriptional regulation..3. The protein can be expressed in nucleus and perform metal ion binding, RNA polymerase II cis-regulatory region sequence-specific DNA binding without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "PWGYEETKTLLAILSSSQFYGKLQTCQQNSQIYRAMAEGLWEQGFLRTPEQCRTKFKSLQLSYRKVRRGRVPEPCIFYEEMNAQ",
    "response#2": "SEASRQRFRQFCYQEVTGPHEAFSKLWELCCQWLRPKTHSKEEILELLVLEQFLTILPEEIQTWVREQHPENGEEAVALVEDVQRAPGQQVLDSEKDLKVLMKEMAPLGR",
    "response#3": "PWGYEETKTLLAILSSSQFYGKLQTCQQNSQIYRAMAEGLWEQGFLRTPEQCRTKFKSLQLSYRKVRRGRVPEPCIFYEEMNAC"
  },
  {
    "reference": "MHDKLLIEGKLVAGKGEALAVFNPATGEQIAAIAQADLHQVDAAVLAAESAFAHWGQTTPKTRATLLLQIADAIEENAEAFARLESLNCGKPYHAALNDEVPAVADVFRFFAGAARCLSGSAAGEYLEGHTSMIRRDPVGVVASIAPWNYPLMMASWKLAPALAAGNCVVLKPAEQTPLTTFYLAHLLAEILPAGVVNIVFGRGADIGDALTGHEKVNMVSLTGSIATGAHILSHTASSVKRTHMELGGKAPVIVFDDADIDQVVDGIRSFGFYNAGQDCTAACRLYVQRAVYDKVVEALGNAVATLKMGDPHDETTELGPLITEQQLERVVGFVERAKALPHITVVTGGERVKGQGFYFQPTVLAGAKQEDEIVQKEVFGPVISITPFDDEAQVIGWANASHYGLASSVWTRDIGRAHRLAACLQYGCTWVNTHFMLVSEMPHGGQKLSGYGKDMSMYGLEDYTIVRHIMIRH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design must be able to perform aminobutyraldehyde dehydrogenase activity, 1-pyrroline dehydrogenase activity in order to contribute to putrescine catabolic process.2. The protein should have a high affinity for NAD(+). The designed protein sequence is ",
    "response#1": "LGGLFQNGLVEWVSSMTYQAASGAGAKNMRELISGMGAVHNQVAAELSDPASAILDIDRKVADFLRSDNYPKENFGVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILNSQQPVIVEGLCVRVGAMRCHSQAITLKLKQNLPVSEIEQIIASANAWTKVVPNTKEASIHELTPAAVTGTLTTPVGRIRKLAMGDEFISAFTVGDQLLL",
    "response#2": "LGGLFQNGLVEWVSSMTYQAASGAGAKNMRELISGMGAVHNQVAAELSDPASAILDIDRKVADFLRSDNYPKENFGVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILNSQQPVIVEGLCVRVGAMRCHSQAITLKLKQNLPVSEIEQIIASANAWTKVVPNTKEASIHELTPAAVTGTLTTPVGRIRKLAMGDEFISAFTVGDQLLR",
    "response#3": "LKPIHEAYGLDRVIVSTYQAVSGAGVEAIEELDQQNASLLKGEKPQAAILPSSGDKNHYQIAYNVIPQIDLFAEEGYTFEEWKMINETKKIMGLPDLKVSATCVRVPVKYGHSESVYIEIDRDDVTVTDLQALLAQADGVIVQDDPANQLYPLATESEDKKETFVGRIRKDPDVANGFHLWIVSDNLIE"
  },
  {
    "reference": "MGGKWSKSSIVGWPEVRDRIRRTNPRAERVGAGYQNLDRHGASANSNAATTSVERAQPEEQEQEEEVGFPVRPQVPLRPMTFKAAFDLSFFLKEKGGLDGLIYSKKRQEILDLWVYNTQGXFPDWQNYTPGPGTRYPLTFGWCYKLVPVDPREVEEANEGENNCLLHPMSLHGMEDDSREVLKWVFDSSLARRHLARELHPEYYKDC",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.2. For general function, the protein need meet that Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.3. The protein should be optimized for stability and expression in virion component, host cell Golgi membrane, host cell plasma membrane, extracellular region where it will participate in suppression by virus of host autophagy.4. The designed protein should contain at least one Dileucine internalization motif; necessary for CD4 internalization, PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "MGGKWSKSSLVGWPAIRERMRRAEPAAEGVGAVSRDLERHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPEKVEEANEGENNCLLHPMSQHGMEDPEKEVLMWKFDSRLAFHHMAREKHPEYYKDC",
    "response#2": "MGQDRNKRSSQNWPWQWEWGTMLLGMLMICSAAEDNXXXRAGXGTISYGQXRXPXIXGMXPFXGXXXDXAXNXRXXXAGXXXGXTXRXRXXHXXXKXPXHXEXXXRXTQXXXIXXXQXAXC",
    "response#3": "MGHHHHHHSSEGL"
  },
  {
    "reference": "MQGGSGVVLKNKKITLGVTGAISAYKALELSRLLIKEEAEVWPVMTRSATEFITPLSLSTLCKNPVSASLFDLTEETRIGHIELAQKADLLIVAPATANFIGKAASGIADDLLTTIACASSAPVLIAPSMNSRMWGNPVVQENLRRLEASGYLFVGPEEGELACGYEGKGRLASVENILEAAREALSPRDLKGEKVLVTAGPTREAIDPVRFVSNSSSGRMGYAIAKAARRRGAEVVLVSGPSYLPRPAGITYIPVTTAEEMLDASVRYFTQSTVVVMAAAVADYRPTKSYPTKVKKEAKFLSIEMERTPDVLKYMGSHKKEGQLLVGFALETDSLEENARKKLKEKELDLVVGNTPAGLDSDFNQVTMIDRDGKKEVLPAMRKDEVADRILDRAARLKK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.2. Ideal protein should have a binding affinity for key enzymes in the coenzyme A biosynthesis pathway to enhance the overall efficiency of the pathway.3. The protein should have a low Km value for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. The designed protein have Flavoprotein domains with specific sequence motifs that are crucial for its activity.5. A protein with metal ion binding, phosphopantothenate--cysteine ligase activity, FMN binding is needed to enhance pantothenate catabolic process in a specific context. The designed protein sequence is ",
    "response#1": "MKVALGVCGGIAAYKACEVVRLLQDAGIRIQVVMTAAAQEFVRPLTFAALSGEKVITGLWQSGGEQPNFDSAIDHISVAQSIDALIVAPCTADTAAKFAHGQADDFLSTLYLATPAPVIIAPTMNVEMLNHSATQANLETLRQRGVHIVEPGSGYLACGMVGAGRLAGPEEIVAAVLAAFDMALG",
    "response#2": "MKVALGVCGGIAAYKACEVVRLLQDAGIRIQVVMTAAAQEFVRPLTFAALSGEKVITGLWQSGGEQPNFDSAIDHISVAQSIDALIVAPCTADTAAKFAHGQADDFLSTLYLATPAPVIIAPTMNVEMLNHSATQANLETLRQRGVHIVEPGSGYLACGMVGAGRLAGPEEIVAAVLAFVEAVLARGPLEGRKVLITSGPTQEPIDDVRFVSNRSSGKMGAALARAALLMGAEVTVVSGPARAALPLQANIVHVRTAQEMLEAGLAHCADANLIIGAAAVADYRPAVRHRGKIRRSAEPTTLELHPNPDIIAEIAKAAPDAITVGFAAEPSRSLDAARDKLARKGLYAIAANDVSRDGIGFDADQNSLRLISADGAEDWPRMPKSEVAARLAARIADHFSG",
    "response#3": "MKVALGVCGGIAAYKACEVVRLLQDAGIRIQVVMTAAAQEFVRPLTFAALSGEKVITGLWQSGGEQPNFDSAIDHISVAQSIDALIVAPCTADTAAKFAHGQADDFLSTLYLATPAPVIIAPTMNVEMLNHSATQANLETLRQRGVHIVEPGSGYLACGMVGAGRLAGPEEIVAAVLAAVEAALGPRDLAGERILVTAGPTREEIDPVRFISNHSSGKMGYAIARQARLRGAEVTLVTGPTAITPPLGVAVIRVESAEEMREAVLARLEETDIVIKAAAVADYRPRLRAQMKVKKTAAHLAIELEKNPDILAEIGTRKEGRVLVGFAAETEDLVANATLKLTLKNLDLVVANDVSQEGAGFHVDTNIAKLVSKGGIEHWPRMGKDVVARALAERIADALG"
  },
  {
    "reference": "MAKQIIVGVCAGISAYKSAELVRLLCKADYTVHVVMTKSAVKFIAPLTFEALTGHRVYTHLFESQHPSAMDHIELARWAQSILIAPATADFIARLRCGIADDLLSTLCLATTAPIILAPAMNQKMWSNPATQDNIQSIQQRGIRLIGPGIGDQACGDTGPGRMVEPQQILDQLSDADAADNLLTKRRVLITAGPTREPIDPVRYISNHSSGKMGYALAHAAINAGAKVTLVSGPVAISSPQTATTIQVQTAKQMHDAVMGHISCQDIYIGCAAVSDYSPAQVSTTKLKKNADTLTVILNKTQDIVRAVASSHADVFCVGFAAETNDLEHYAREKLRDKQLDMIIANQVGIADGGFSSDNNAASVLWNNGQQTFSLRSKKRLAVDIIKIIAQHYYASKHTT",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be able to perform phosphopantothenoylcysteine decarboxylase activity, metal ion binding, phosphopantothenate--cysteine ligase activity under specific conditions.2. The Proton donor of the protein must be engineered to enable effective catalysis of H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.3. Mg(2+) is required for the proper folding and stability of the protein.4. A protein that is capable of catalyzing reactions within the coenzyme A biosynthesis pathway with high efficiency and specificity.5. The protein contains novel DNA/pantothenate metabolism flavoprotein C-terminal domains that confer a unique function or activity.6. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A. The designed protein sequence is ",
    "response#1": "MKIALGVSGGIAAYKAAEIVRLLQDRGIRVQVVMTAAAQEFVRPLTFAALSGEKVITSMFGSGGDEQANVESAIEHIAIAQAIDALVVAPATADVLARFAQGIANDFLSTLYLATTAPVVVAPAMNVNMWNHVATQANLEILRKRGVRIVEPGSGYLACGMTGAGRLAEDETIVAAVMEAAHLLASSQDLKGKRIVITAGPTREALDPARYLSNRSSGKMGYALARTAARRGAEVTLVSGPVDIKAPSWVATIPVKTARQMHARVLELSEDADIIVKAAAVSDFKPKNYCRQKIKKNQGLTSIEVEENPDILSDLGESRRRGSVLVGFAAESRNHEAEGRRKLLQKKTDLIVVNDILSSKTGFDVDTNQVILLFANGDEEPLPIASKTQLAQRLLNLIAQRLNQ",
    "response#2": "MKIALGVSGGIAAYKAAEIVRLLQDRGIRVQVVMTAAAQEFVRPLTFAALSGEKVITSMFGSGGDEQANVESAIEHIAIAQAIDALVVAPATADVLARFAQGIANDFLSTLYLATTAPVVVAPAMNVNMWNHVATQANLEILRKRGVRIVEPGSGYLACGMTGAGRLAEDETIVAAVMEALAARPGARPLAGETVLVTAGPTREKIDPVRYLTNRSSGRMGYAIAEAALRRGARVLLVSGPVAVTAPDAAEVTRVETAEEMLAAVLGLLPQSTVVIKTAAVADFRPKRAAGQKIKRKGEMTLELEPTADILAQVARRKTTQIVVGFAAETENVLENARKKLASKSLDAIVVNDVSSEGVGFDSERNAVTIALLDRLGRAVVIVAGSMAAVKAPMVLRRLREAGFATRALATRAALAFVTELSLATAADAPVLTDESWFAPSPEAQHLALARCDLVVVAAGSADLMARHVSGRADDLASATLLSTRAPVLWAPAMNPSMWEHPATQHNLRTLLEWGHEFIGPVFGALGSSGEGEGFGRMAEPEQIAARVLDLLVRRR",
    "response#3": "MKIALGVSGGIAAYKAAEIVRLLQDRGIRVQVVMTAAAQEFVRPLTFAALSGEKVITSMFGSGGDEQANVESAIEHIAIAQAIDALVVAPATADVLARFAQGIANDFLSTLYLATTAPVVVAPAMNVNMWNHVATQANLEILRKRGVRIVEPGSGYLACGMTGAGRLAEDETIVAAVMEAVDLLVTPKDLAGETVLVTAGPTREKIDPVRYLTNRSSGRMGYAIAEAALRRGARVLLVSGPVAVTAPDAAEVTRVETAEEMLAAVLGLLPQSTVVIKTAAVADFRPKRAAGQKIKRKGEMTLELEPTADILAQVARRKTTQIVVGFAAETENVLENARKKLASKSLDAIVVNDVSSEGVGFDSERNAVTIGFWTKQGKKVAIPKMSGKHVLITLGPTREKWDAVRFWSNPSSGLMGACIAMAAWLRGAKVTVVSGPVNWWFPEDINVIKVNSAQQMFDAATEVWPSCTTGCFTAAVADFKPIPHGEGKFKKEGSSALRVDFDTNPDILKTIGSMKKDDQQLIGFAAETSNIQEAAKGKLERKNLDLIVANPINKPGAGFESSTNSVYDVAVLPDGTTQRLPKSQKSEIAMAIVQSVANVLNG"
  },
  {
    "reference": "MLGAIREPPIDVQIALHSRDDNQTGLVLRGTRRTVDRVLKGLCSSPCFFCSVSLTMATLTTTMATTATMATTEASKPLEAQARTALTKATNYAWEIFSNRHWCGELESNVTVTCEHIFFLYVLYQHIDPGEGSQYRQWLLSQQNSDGSWGIAPNYPGDISTSAEAYLALRIIGMSTDSPELYRARTFIRAAGGLSKMRMFTRIFFAEFGLVPWTAIPQLPAEFILVPAHFPISIYRLASWARSNVVPLLIIAHHRPLYPLPNGLHKQNPFLDELWLDPATKPLPYGSSDPTDPVAFVFTILDKALSYLGGLRRSPTRGYARRRCVQWILQHQEKAGDWAGIIPPMHAGIKALLLEGYKLHDEPIQLGLAAIERFTWADNRGKRLQCCISPVWDTVLMIRALQDTPASLGIKLDPRIADALAWTAENQHRGPEGDWRVYKPNIPVGGWAFEYHNTWYPDIDDTAAAVLAFLTHDPATARSRLVRDAVLWIVGMQNADGGWAAFDHENNQLFLNKIPFSDMESLCDPSTPDVTGRTIECLGMLRDLLMRPAENAENGEKYGYPDGEGDAAADAHLLQIINTACARAIPYLIRSQEATGTWYGRWAVNYVYGTCLVLCGLQYFKHDPKFAPEIQAMAARAVKWLKQVQNSDGGWGESLLSYREPWRAGCGPSTPSQTAWALMGILTVCGGEDRSVQRGVRHLVDTQDDTLSQGDGGAAAWTEREFTIREPLHEASQRIGSD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Fumihopaside B, another minor derivative produced, is different from fumihopaside A due to the presence of a double bond between C-22 and C-29.; Squalene hopane cyclase; part of the gene cluster that mediates the biosynthesis fumihopaside A, a hopane-type glucoside that enhances the thermotolerance and UV resistance of N.fumigata; AfumC is also able to accept UDP-D-galactose and UDP-D-glucuronic acid as donors to yield minor derivatives2. The protein should be designed to localize specifically to lipid droplet in order to perform its role in triterpenoid biosynthetic process more effectively.3. The Proton donor active site of the designed protein must be optimized for substrate binding and specificity.4. A protein that is capable of catalyzing reactions within the Secondary metabolite biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "VPYAHLTDLTQSLQHDLATLGISVGSKVAIVLPNGLAFVTVLLATIRQRAISAPIHPNSTREECKQIFSLMTPDLVAVMPSEASPAAAAVLAAQDLGLPVASCHRYPQSGRLSFCLALKLVNRGDETSNPSPCMVYSRDHVLAEDKVLELFTSGTTGAPKSVQLTHTNILVAMRIITSAHKITFKDRSFLITPLFHIIGIAGSLLPTLFTGGCAVIPASLPATFWQDCQDYSITWYHAVPTLHHLLLSFPMPKGGVPATLRFIRSGGSDMSLDLFNRLQKLGVPLLEVYGMTETAPAIFCNPFPVTETSTAIKRHPGQYPIPDAVDVMILPPERAPGLEPNGDLDGIENTEPVARLTKELGVKGEICLRGKNIMAGYWKNPEKTAETIVDGWLHTGDIAVMDENGFCRIVDRKKDMILVSGFNVYPNELEEVIYTAPGIKEACVVGVPDPYTGEAVKLFIVPKTQLDEKALIEYCRDKLTGYKVPKYIEFRDELPKTNVGKILRRELREL",
    "response#2": "LLDGADLSNGSPFVVDVGGHHGIDLMRVAEKHPDLPAGSLVLEDLPDVVGAVHLTTDKIRTVAHDLFEEGVEQPIKGARAYFMHAVLHDWSDETSVKILRQIAAVMKPGYSKVLINDIVIPSTGASCYQAAMDCLVLQASANERTEAVWSKVIKDAGVTGFDNLAVPYAHLTDLTQSLQHDLATLGISVGSKVAIVLPNGLAFVTVLLATIRQRAISAPIHPNSTREECKQIFSLMTPDLVAVMPSEASPAAAAVLAAQDLGLPVASCHRYPQSGRLSFCLALKLVNRGDETSNPSPCMVYSRDHVLAEDKVLELFTSGTTGAPKSVQLTHTNILVAMRIITSAHKITFKDRSFLITPLFHIIGIAGSLLPTLFTGGCAVIPASLPATFWQDCQDYSITWYHAVPTLHHLLLSFPMPKGGVPATLRFIRSGGSDMSLDLFNRLQKLGVPLLEVYGMTETAPAIFCNPFPVTETSTAIKRHPGQYPIPDAVDVMILPPERAPGLEPNGDLDGIENTEPVARLTKELGVKGEICLRGKNIMAGYTDPDKTAEAFKDGWFYTGDVGFVDDDDYLFLVDRKKDIIIAGGEEIEHVARLHDQVNDAACFRISDEIYGEVIGLAITAKSAITITAVKKHMRHHVVMFKVPDKVLLVQEIPYNRTGKILRRELRNML",
    "response#3": "VPYAHLTDLTQSLQHDLATLGISVGSKVAIVLPNGLAFVTVLLATIRQRAISAPIHPNSTREECKQIFSLMTPDLVAVMPSEASPAAAAVLAAQDLGLPVASCHRYPQSGRLSFCLALKLVNRGDETSNPSPCMVYSRDHVLAEDKVLELFTSGTTGAPKSVQLTHTNILVAMRIITSAHKITFKDRSFLITPLFHIIGIAGSLLPTLFTGGCAVIPASLPATFWQDCQDYSITWYHAVPTLHHLLLSFPMPKGGVPATLRFIRSGGSDMSLDLFNRLQKLGVPLLEVYGMTETAPAIFCNPFPVTETSTAIKRHPGQYPIPDAVDVMILPPERAPGLEPNGDLDGIENTEPVARLTKELGVKGEICLRGKNIMAGSWGMTGRTVYGDHQRFIDTYLKPYPGYYFTGDGAARDHDGYYWIRGRVDDVVNVSGHRLSTAEIEHVARLHDQVNDAACFRISDEIYGEVIGLAITAKSAITITAVKKHMRHHVVMFKVPDKVLLVQEIPYNRTGKILRKVLRQIAAGEAPNLGDISTLADPSVVAKLAALKK"
  },
  {
    "reference": "MSDDNKTTHFGYTEVPVEEKVKRVADVFHSVAAKYDVMNDLMSGGVHRLWKRITIESSGARAGHTILDIAGGTGDLTKKFSRIVGPSGKVVLADINASMLNVGRDKLINSGVSGNVEYCQANAESLPFADNTFDIITIAFGLRNVTDKDAAIRSMLRVLKPGGKLMILEFSKTNNPLLTKAYDFYSFNILPKMGEVIANDAESYRYLAESIRMHPDQETLKQMMDDAGFVNCRYRNMTGGVVALHTGIKP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a binding site that is specific to the target S-adenosyl-L-methionine ligand.2. A protein with S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity, demethylmenaquinone methyltransferase activity functions is required to enable menaquinone biosynthetic process, methylation.3. A protein should be able to manipulate the activity of enzymes in the menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2 pathway, either by activating or inhibiting their function.4. The desired protein should be able to perform a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine The designed protein sequence is ",
    "response#1": "MTAPLHLIVAAGSGTRAGGALPKQWQPLAGQPVLAHTLTALTGHPGITRVVVAIHPDDLARARALAGDNVTLVEGGASRDASVRRGLEAVRADVYDVMNDLMSFGVHRLRPFVSAAVIDRLLAALDSAPGAIPALPVSDTLKREDGGAVTGTVPRAGLFGAQTPQGFPYAAILAAHRAHAGHALTDDAAVAEWAGHPVALVQGDARNLKITRPEDLALAERVLAGAGEAPRAEGVAPDA",
    "response#2": "MDAPQTSFGFREVPVEEKARKVAEVFHSVAPRYDLMNDIMSVGAHRYWKRFTIYVSGLRPGDRVLDIAGGTGDLARAFARKAGPSGRVVLADINAAMLEVGRERLTDDGLLHNVEYVQANAESLPFPDNTFDCITIAFGLRNVTDKDAALRSMHRILKPGGRLLVLEFSKPRNPLLSRAYDAYSFKALPLMGRLVAGDAESYRYLAESIRMHPDQETLRGMMEAAGFERCDYFNLAGGVVALHRGIKA",
    "response#3": "MTDVVTKEQLDELQADDSLKLEWKTDDGKIIVTPIKELCDKWPQYV"
  },
  {
    "reference": "MSGEDIVEIYTDGACSGNPGPGGWGVLMIYKDREKELCGGEQATTNNRMELMAAIQALEALKRDAHVRIHTDSNYVKDGITKWIHGWKKNGWKNAAKQPVKNAELWRRLEAAISTHQVSWHWVKGHSDHPENDRADALARQGMAPYLPSK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+) binding.2. A protein with RNA-DNA hybrid ribonuclease activity, nucleic acid binding functions is required to enable RNA catabolic process.3. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "MKEVTIYTDGACSGNPGPGGWGAVLIYGDKRKELSGAEPSTTNQRMEITAAIAALRVLKEPCRVHLYSDSAYLVNAFRQGWLARWERNGWLTVKKQPVENQDLWRELLQVASRHQVEWLKVKGHSDNPENNRCDELARAAIA",
    "response#2": "IRKHVEIFADGACRGNPGPGGWGAVLRYHGKEKELSGYAEYTTNNQMELAAVIQALRALKEPCRVTITTDSRYLRDGISLWIHKWKQNGWKTRVKTDVRNKELWIALDEACLPHEIDWQWVKGHSGHPENERCDALARAAIV",
    "response#3": "MKEVTIYTDGACSGNPGPGGWGAVLIYGDKRKELSGAEPSTTNQRMEITAAIAALRVLKEPCRVHLYSDSAYLVNAFRQGWLARWERNGWLTVKKQPVENQDLWRELLQVASRHQVEWLKVKGHSDNPENNRCDELARAAII"
  },
  {
    "reference": "MIRVYAPASIANIGVGFDTLGMSISPVNGDKLGDFVSIKDADNFSIKISGLFYDQIPIKLEQNIVFKCWKRFCEISNQIRPVEIKLEKNVPVESGLGSSACSIVASLVAINNYYGNLLNNDQLLQLMGEMEGKVSGSIHFDNVAPCFLGGMRIFLSKYKNTSQLIPSFDNWIWIIAYPGIKVSTVKSRSVLPKQYSLQDCVDYGQYLSGFIHACHTRQELLAIQCIEDIIAEPYRAKLLPVDLSFLRSCVKEKGAVSCGISGSGPTIFVLCNVKNVVDDISNWLSRFYLQNSSGFVKVCSLNKLGSDIVLG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design must be able to perform ATP binding, homoserine kinase activity in order to contribute to threonine biosynthetic process, phosphorylation.2. A protein that can modulate the overall flux of metabolites through the L-threonine biosynthesis, Amino-acid biosynthesis, L-threonine from L-aspartate: step 4/5 pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MKVMKFGGSSLANAKRFQQVAQLALNSVEGYSLALVLSAPQGVTNSLEAAVRRAVGGFNDGLLEPVEAKLAELLQGAQLDAKAAGALEAQWRQELDGVRQRLQGVALLGRCPDEVIAEVMVVGERLSVHLMLALLAQSGERCALLEPRRYLMAHGEPLDSQVDIEASRARLASLPVGEYRFWVMPGFTASNEMGQVVTLGRNGSDYSAAALAACLHADSCEIWTDVDGVYSVDPRLVPDAKLLEQMSYLEAMELSYFGAKVLHPRTIAPIAHNQIPCRIRNTLNPEAPGTLIHKDGSADQPVKAVSHLAKQTMISVSGPGMKGMVGMAARVFDAISLAGVSVSLITQSSSEYSISFCVDAKDQPRAVKALESTFALELKSEMLEPLELKPNLAIVTLVGEGMKTRKGVAARFFRSLAQGSINIAAIAQGSSERAISAVINNDDAEAALRICHQNFFDSRMFVDVLLVGCGQVGRELLGQIQQQQSALSKQQLALRVCAIANSRQMLLDRHGLELAHWQDKLASGGQPMDLDALFQFAHDAKLTNPVLVDCSASGDLALRYLDAMNSGLHVVTPNKKANTGDYDYYQALKHTAQRQRRQFLYETTVGAGLPVLDNLQGLMRAGDELKRFSGILSGSLSYIFGLLEEGHSLSEATGLAKDKGFTEPDPRDDLSGMDVARKVLILAREAGMALEMSDIEVESVLPAEFDASGDVASFMANLPNADERVAARVAEAKANGQVLRYVGAIDTDGRCKVSVQAVGADHPLYA",
    "response#2": "VTLREGGTPLVPAQVLSERTGCEVHLKVEGANPTGSFKDRGMTMAITRAKEEGAKAVICASTGNTSASAAAYAVRAGMVCAVLVPQGKIALGKMGQALVHGAKILQVDGNFDDCLTLARSLSDNYPVALVNSVNPVRIEGQKTAAFEIVDALGDAPDIHVLPVGNAGNITAYWKGYTEYARDSVSTHTPRMWGFQASGSAPIVRGEIVKDPSTIATAIRIGNPASWDYALAARDESGGFIDEVTDRQILSAYRLLASQEGVFVEPASAASVAGLLKAAEEGKVDPGQRIVCTVTR",
    "response#3": "MRVLKFGGSSLASVTRFLEVADIVQQQIGNQSVGLVLSAPQGVTNLLVELTDLAFLGSDYQPLLLQWKNRLAQHIAEASLRVNAQQLKAVQQLADEQEQKLTERLQGISLLSYCPDHIKAQILGMGEQFSVCLMTALFKSRQVNVVLLDSVALVKSQGDYLNAVADIPASEQSFAAALAKQSAQVYVMAGFVSSNAQGELCLLGRNGSDYSAAIVAAAVKASVCEIWTDVDGVYSADPRQVKQAKLIDHLSYDEAMELSYFGAKVLHPKTIGPLARFQIPCYIKNTLNPSAPGTCIDADNNGDSLVKGISSLEHLALITVSGPGLKGVVGMASRVFATMAREQVSVSLITQSSSEFSISFCVAQLDLAAAKKALEAEFELELQGKLLRPLQILSDMAIVSLVGDGMRQHRGVAAKFFASLAQARVNVVAIAQDSSERSISAVVEQKACQNAVKVCHENFFSHVPSIDVFLVGCGVVGSELLAQFQRQQEFLQQRNVKLKVYGIANSKALLLDEKGIDLSQWQQQMQQAKAQFSVADLSRFAQEQHLTNPVLIDCSSAEPVADLYVDFLKAGFHVVTPNKKANTGSLDYYHQLRQVAQQHRRRFLYETTVGAGLPVIDTLQGLFNAGDELVAFEGILSGSLSYIFGLLEQGQSLSEVTTKARELGFTEPDPRDDLSGMDVARKLLIMARESGLMLNLADVEVEGVLPADFAPNTDVPTFMQKLPQLDKAFSERIAQARAKGQVLRYIGEISEGRCKVAIKALDLDHPLL"
  },
  {
    "reference": "MPSRMGSKMNLNRDCIRIKTHYDGDILITNLDASMNYFELCDEVREMCNLQQEQPITLKWIDDEGDPCTISSQMELEEAFRLYCQRRDEGLIIHVFPSIPEKPGMPCPGEDKSIYRRGARRWRKLYRVNGHLFQAKRFNRRAYCGQCSERIWGLGRQGYKCINCKLLVHKRCHILVPLTCKRHMDSVMPSQEPQLNDKNDEVDLPSEEHGGIPYIPSARKHDTIKDDSEDIKPVIDGMDGIKISQGLGLQDFDLIRVIGRGSYAKVLLVRLKKNDQIYAMKVVKKELVHDDEDIDWVQTEKHVFEQASSNPFLVGLHSCFQTTSRLFLVIEYVNGGDLMFHMQRQRKLPEEHARFYAAEICIALNFLHERGIIYRDLKLDNVLLDAEGHIKLTDYGMCKEGLGPGDTTSTFCGTPNYIAPEILRGEEYGFSVDWWALVVLMFEMMAGRSPFDIITDNPDMNTEDYLFQEGFTNQNNSRIILLDPKERLGCQLQTGFSDIKSHTFFRSIDWDLLEKKQATPPFQPQITDDYGLDNFDTQFTSEPVQLTPDDEDVIKRIDQSEFEGFEYINPLLLSTEETV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein with the reaction ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-   threonyl-[protein], the Proton acceptor must be optimized to ensure efficient catalysis.2. The designed protein should contain one or more Phorbol-ester/DAG-type, PB1, AGC-kinase C-terminal domains that are essential for its function.3. The designed protein should have metal ion binding, protein kinase C activity, ATP binding within vesicle, microtubule organizing center, apical plasma membrane, nuclear matrix, apical cortex to regulate positive regulation of interleukin-13 production. The designed protein sequence is ",
    "response#1": "MAPFLRLSFNSFDLGGMLSPSEHIQPFCAVKVKESLTTERGKTLVQKKPTMYPDWKSPFDAHIYEGRVIQIVLMKAVEDPLSEATVGVSVLAERCKKGNGKSEFWYN",
    "response#2": "MAPFLRLSFNSFDLGGMLSPSEHIQPFCAVKVKESLTTERGKTLVQKKPTMYPDWKSPFDAHIYEGRVIQIVLMKAVEDPLSEATVGVSVLAERCKKGNGKSEFWYKL",
    "response#3": "FDIGKLLGMGRFGHVYLAREKKSQFIVALKVLFKNQLQTHNIEHQLRREIEIQSHLRHPNILRLFGYFYDDKRVFLIIEFAKGGECFKELQKVGSFNEQTAATYTLQIADALRYCHSKHVIHRDIKPENLLIGVGGEIKIADFGWSVHAPNTKRSTFCGTLEYLPPEVIEKKGYDQTADVWSLGILIFEFLVGRSPFTSDEEKNIFHNIQENDVYYPSSISPEAKDLISRLLVSDPHQRITLKDVINHPWI"
  },
  {
    "reference": "MLVPPPNYGMVEENFYRSGQPDQLNFPFLEKLGLKSVIWLAPEEPEPGFLDFCVDQNIELHHLGVLYSTNAWDPITEEVVLQALHLLVQPATYPVLVMCNLGRHRTGTVVGCFRKLQRWNLSAILEEYRRFVGGQKYRILNEQFIELFDEELVFGASY",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The desired protein should be able to perform H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] +   phosphate2. A protein with protein tyrosine phosphatase activity, phosphatase activity is needed to enhance protein dephosphorylation in a specific context. The designed protein sequence is ",
    "response#1": "WQMIWQEKSPVVVMVTNVVELGKRKCHKYWPDESAQYGSIKVSQTHIEDLCDYTIRTFVIQQFDGYDVREVKQFHFTAWPDHGVPTRPTNILSFIRRVKSYETPGQGPTTC",
    "response#2": "WQMIWQEKSPVVVMVTNVVELGKRKCHKYWPDESAQYGSIKVSQTHIEDLCDYTIRTFVIQQFDGYDVREVKQFHFTAWPDHGVPTRPTNILSFIRRVKSYETPGQGPTTC",
    "response#3": "WQMIWQEKSPVVVMVTNVVELGKRKCHKYWPDESAQYGSIKVSQTHIEDLCDYTIRTFVIQQFDGYDVREVKQFHFTAWPDHGVPTRPTNILSFIRRVKSYETPGQGPTTC"
  },
  {
    "reference": "MTGIIRNKVFFQIKQGNTPLGRVVFELYNDIVPKTAENFRALCTGEKGIGKSGKPLHYKGSSFHRVIKNFMVQGGDFTHGTGIGGESIYGRTFNDENFLVKHKIGCLSMANAGKNTNGSQFFITTAETPHLNGGHTVFGEVVEGFDIVKKVENAETDRSDRPKAACVIEDCGQL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides .2. The designed protein should be involved in protein folding by localizing to intracellular membrane-bounded organelle, mitochondrion and exhibiting cyclosporin A binding. The designed protein sequence is ",
    "response#1": "YFDVEADGQPIGRVVFKLYNDIVPKTAENFRALCTGEKGFGYAGSPFHRVIPDFMLQGGDFTAGNGTGGKSIYGGKFPDENFKKHHDRPGLLSMANAGPNTNGSQFFITTVPCPWLDGKHVVFGEVVDGYDIVKKVESLGSPSGATKARIVVAKSGD",
    "response#2": "FFDIEHGEEKVGRIVIGLYGKVCPKTAKNFYKLSTTTNSKKGFIGSTFHRVIPNFMVQGGDFTDGTGVGGKSIYGDTFPDENFTLKHDRKGRLSMANRGKDTNGSQFFITTTEEASWLDGKHVVFGQVVDGMDVVNYIQHVSRDANDKPLEAVKIAKCGG",
    "response#3": "FRIVLDGYNAPVTAGNFLDLVERHFYDGMEIQRRDGFVVQTGDPEGPAEGFIDPSTEKPRTIPLEIMVEGEKVPVYGSTLEELGLYKAQTKLPFNAFGTMAMAREEFENNSGSSQIFWLLKESELTPSNANILDGRYAVFGYVTDNQDYLADLKVGDVIV"
  },
  {
    "reference": "MRLVTAGQMRNIDANTINKFGIPGIVLMENAALAVVRQVRKILHGERQPKIPGKKAVILIGKGNNGGDGFAVARHLSVSGMEVTVFFFAGGGELGGDARLNYELYLKMGGKIVQVEDEMQIRLFRLALIQADIVIDALYGTGFKGSLPDLTQKYVAEMNKCSAPVLAVDIASGLEADSGKVSSTAVKADYTVTFGLAKLGHFLGEGPVYTGELLIDQISIPENIIYEEKITTYLLTAEIIQPLIPVRNRQGHKGTHGTGVFIGGSVGMSGAVVLGGRGALRSGIGLLQMVVPEGIVETVDRGVIEGTVWPAPDYEMLGQGAWNAVLERLTEADACAVGPGLKPNSGFLPVIKSILADTSVPVILDADALNLVAGQPELLSLRKGQGSLVVTPHPGEMARLCQCTVLEVQESRVELALAKAIEWGAIVVLKGAVTVIASPDGRVFLNPTGNPGLGTGGTGDVLTGCIMAYAAQGVPLLGAACLGTFLHGRAADELSGIYGSAGFTAAEVADMLPKVRKTLEVEE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein have YjeF N-terminal domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "SPDLWRAHLPKPDLASHKYDRGHTLVASGGATRTGAARLAAVAALRIGSGLVTIASPPEALGVNAAHLTAVMLRGCDGPEGLYHILKDPRFNALVLGPALGIHANTRAMVAVAVQAQRSLVLDADALTSFEDLSSELHQAFRQAPTVLTPHEGEFKRLFSGHPDLLDPPSKVERTRRAAAYTGAVLLLKGSDTVIAAPDGRAAINENGTPYLGTAGSGDTLCGIIAGLMAQGLPAFEAACAGTWIHAEAGAGFGPGLISEDLAGLLPGVLRDLLA",
    "response#2": "SPDLWRAHLPKPDLASHKYDRGHTLVASGGATRTGAARLAAVAALRIGSGLVTIASPPEALGVNAAHLTAVMLRGCDGPEGLYHILKDPRFNALVLGPALGIHANTRAMVAVAVQAQRSLVLDADALTSFEDLSSELHQAFRQAPTVLTPHEGEFKRLFSGHPDLLDPPSKVERTRRAAAYTGAVLLLKGSDTVIAAPDGRAAINENGTPYLGTAGSGDTLCGIIAGLMAQGLPAFEAACAGTWIHAEAGAGFGPGLISEDLAGLLPGVLRDLLR",
    "response#3": "SPDLWRAHLPKPDLASHKYDRGHTLVASGGATRTGAARLAAVAALRIGSGLVTIASPPEALGVNAAHLTAVMLRGCDGPEGLYHILKDPRFNALVLGPALGIHANTRAMVAVAVQAQRSLVLDADALTSFEDLSSELHQAFRQAPTVLTPHEGEFKRLFSGHPDLLDPPSKVERTRRAAAYTGAVLLLKGSDTVIAAPDGRAAINENGTPYLGTAGSGDTLCGIIAGLMAQGLPAFEAACAGTWIHAEAGAGFGPGLISEDLAGLLPGVLRDLLE"
  },
  {
    "reference": "MMNAIIDELKVFTGNAHRGLAKAVCGYLDIPLGQAEVFKFANDNTFVRIQENVRQRDVFIIQPTCAPVNDNLMELLIMVDACKRASAGRITAVVPYYGYGRTDKKTQPRVPITARLVADLLTAAGADRLLTVDLHAGQIQGFFNIPVDELTAMPMLADFFKAKELSDLVVVAVDIGISKKARDVAERLHAPLAIIEKRRLGNDEHSEIMNVIGDVEGKIALTFDDEIDTGGTITNAAVALAEHGVKEVYCCVTHPVLSRGGAENLAKSHFKEVVVADTIPLPPEKRNGKITVLSVAPLLGEAIYRIHKGLSVGEMFN",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining ribose phosphate diphosphokinase activity, magnesium ion binding.2. The protein must exhibit the following characteristics: Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P)..3. A protein that is capable of catalyzing reactions within the 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis pathway with high efficiency and specificity.4. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+) binding. The designed protein sequence is ",
    "response#1": "MKIFAGRAGRNLANLMCDHLNIPLAEGHTELFPDGELIVKVNEDVRGRDCFVVQSTCDPVNANLMELLIYIDCLRRASAKRITAVIPYFGYARQDRKDEGRTPITAKLVANLIATAERGVNRILTVDLHASQVQGYFNIPVDHLYAPVLVRDIQARLKLDNVMVVSPDVGGVVRARALAKRIDAPLAIVDKRRERAGESEVMNIIGDVDGKICILIDDIVDSGGTLCNAADALRAQGASDVYAYITHGVLSGGAAAAAPVIVNYFQDNPIDNLVVVAPDTGGAERARAYAKRLDAGLVLVDKRREKANVAEVMNVVGEVDGMNCLIVDDMCDTGGTICKVAAALHKNGANEIYACFSHGVLSGNAIKNLRGSHIKKIIVTDTIPLHEEASLDKITTLSVAPLFAEAIHRINNDLSISAMFE",
    "response#2": "MKIFAGRAGRNLANLMCDHLNIPLAEGHTELFPDGELIVKVNEDVRGRDCFVVQSTCDPVNANLMELLIYIDCLRRASAKRITAVIPYFGYARQDRKDEGRTPITAKLVANLIATAERLGADR",
    "response#3": "MKILSGNSNRPLTEAIAAYLGLPLTKCQVRRFADMEIFVEIQENVRGQDTFIVQSTSYPTNDHLMEMLIMTDALRRASARRITAVLPYFGYARQDRKPGPRTPISAKLVANLITRAAERVGDLHAGQIQGFFDIPTGPDVGAAPVIVNYFQDNPIDNLVVVAPDTGGAERARAYAKRLDAGLVLVDKRREKANVAEVMNVVGEVDGMNCLIVDDMCDTGGTICKVAAALHKNGANEIYACFSHGVLSGNAIKNLRGSHIKKIIVTDTSPLDAEAARLPGIRIVPAAELLGEAIRRVHNRQSVTELFR"
  },
  {
    "reference": "MEKACKEPEEQPQSPPKADEERPSVEPSPEKSSPEEQSSEEVSSEEEFFPDELLPELLPEMLESEERPPQERLSRKDLFEARPPMEQPPCGVGKHKLEEGSFKERLARSRPQFRGDIHGRNLSNEEMIKVAEEMEEMKRVRNKLMVMHWKARRNRPYPI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be expressed specifically in the nucleus2. The protein must exhibit the following characteristics: May be involved in transcriptional regulation. The designed protein sequence is ",
    "response#1": "FSDDQLVSMTVRELNRHLRGFTKDDVIRLKQKRRTLKNRGYAQSCRYKRVQQKHNLEGEKTQLVQQVEQLKQEVSRLARERDAYKIKCEKA",
    "response#2": "FSDDQLVSMTVRELNRHLRGFTKDDVIRLKQKRRTLKNRGYAQSCRYKRVQQKHNLEGEKTQLVQQVEQLKQEVSRLARERDAYKIKCEKL",
    "response#3": "FSDDQLVSMTVRELNRHLRGFTKDDVIRLKQKRRTLKNRGYAQSCRYKRVQQKHNLEGEKTQLVQQVEQLKQEVSRLARERDAYKIKCEKL"
  },
  {
    "reference": "MYNYFYQGHLVRKCLGQHFLHDQNIIESIVAVIHPLPSQALVEIGPGLGALTKYVAKYVKTITVIELDHNLVAYLANHPILQHKLNILSQDVMKVNFSDLAKKLSQPLRIFGNLPYNISIALMFNLFRHIHMIRDMHFMLQKEVVSRLLAKPNNKNYGKLSVIAQHYCQIDLVLDVPPESFRPVPQVDSAVVRLVPYVIPPYPVKDINKLYLLTSLAFQQRRKTIRNSLRNLFSVEQLLTQGIISTLRAENLSVEQYCCLASTLAECLPKK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be optimized for 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity. The designed protein sequence is ",
    "response#1": "ILQKIVDTAEIDQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLVPILADTLRDFDNVQVVNQDILKADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRISAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRR",
    "response#2": "VIDAIVRAIAPARDDAIVEIGPGLSALTAPLIARLNRLSVVEIDRDLAARLRKKYPPEQLSVVEADALTVDFRQFGAGMRVVGNLPYNISSPLLFHLMGAADLVRDQHFMLQREVIDRMVAEPRSADYGRLSVMLQSRYRMEKLFDVPPEAFDPPPRVVSAVVRMVPLL",
    "response#3": "IIHGIVNAISPQAGDVLVEIGPGLGALTDPLLERIPQMQVVELDRDLVERLRRRYGERLQVHAGDALDFDFDKLAVPGRPLRIVGNLPYNISSPLLFHLMEFADHVHDQHFMLQKEVVERMVAEPGSKAFGRLSIMLQVRYYMEHVLDVPPGAFNPPPKVDSAVVRMIPWR"
  },
  {
    "reference": "MTEQQPIRQSSYNKKELEASGRGELFGKNGPPLPSGNMLMIDRVISMSESGGKFDKGFIEAELDIDPKLWFFECHFTNDPVMPGCLGLDAMWQLVGFYLGWLGGEGKGRALGVGEVKFTGQVLPTSKKVTYKIHFKRVINRRLIMGIADGEVFVDGKLIYEATDLKVGLFKDTENF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Necessary for the introduction of cis unsaturation into fatty acids; Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E- (2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP; Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length..2. The protein should be optimized for activity in fatty acid biosynthesis, Lipid metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The designed protein must possess 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity, 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity, (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity to support fatty acid biosynthetic process under certain conditions.4. The active site active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "MCAQHNSSSSSQNGNAPPLTIGFVIAEFNRDLTEAMAERALRAARSYGATSEVVRVPGSFELPAALKRLAESAKYDAVIALGAVIRGSTDHYNVIVNEASRGIAQVALDSDTPIGNGVLTVENEEQALERSGTKAGNKGWEAALSAIHMANLVKNL",
    "response#2": "MATLSKKKQLAMYKKYAGAGRKDRPYRIPDTPFIETHDWARLNEALGDNYFRYVKQNKPKGHDVIDDDGTVIPINDLKAATLKLLSDMSQIGGQLSVYPAPVHDYFPTMPMRLTTQRALKELHTDYPNADIETILGEINSQLQELEDAYNESHPNDPESKRNREEKAILARCDELDKEIAAHETHPVFYTYYSLHLWGLDLRVLGLMYRHTGHIDPSHSEAITAQASRILRALGDVTEDLLAGEDPYGTGLTPEQRAWLDAEFDTPLADADSFPYSKHDYDGWVRLNHFQNDDFRSSPAKRERLEKIATEAVTALDLPDGFTIDVHPYTHQVFGSPPMSASSRDLLSTRENIDINDLHKIVGEHNELLDELYEQYRAKRGYVNIYDGDAIPAPASTVDAYMTGYYTRAGDVSGGSDYINVYVPSHPDIRPLVEWGTSIHEARPGHHTQAIQSELQFEDLPLYRRFGYYSAFAEGWGLYAETLGKEMGLYTNTYDRLGYLSYEMWRACRLVVDTGIHAFGWTRQEAIDYLADNTAMSRHNVSNEVDRYITWPGQALTYKTGELKILELREKAQNELGDSFDLKDFHDVVLKNGSVPLPILERHIDEYIESV",
    "response#3": "MKSTISHTEPITMDTLEKIQHLADSLWDQTEYRKGIEGTAYHTGNPDKATVLVVGNEAAGLTACGKKTQTHFPVSSEHPGTHYYKVQVYPKDGESYFTLSISRDGKTLRSHRYDHNNGHPLQVSLEALRAPKLLDDNMVPYRLVREDEHTGEHRYIGICHDGQEAAYTHAGQKIKLSRFVQQFPDYDLVVTQGVYQTYPENAPAQCLIAVGDNLCIAGSKNPKTHFGVDVAGHEWYRPVATVSKKAHRYVVLTAERSIAVYTRNNEPIKTVPYDTHMSVWKNMADIIGKMRQLAPEIEKAEAQEVEDCVRKAYRDWNDGKPLGERGMSILYGILHQTSPEEQAEILAEALDRLLETTPELERYLKAEALLKIEQEIAGNDRNSES"
  },
  {
    "reference": "MQGIWTTERIRAAEDLLLARTPDGELMRRASFGLAAEVAGLLAEDTGGVSGRRVVLLVGSGNNGGDALWAGAFLRRRGVAVAAVLLKPEKAHPAGLAALRRSGGRVVSIADSAPWIEQADVVVDGIIGISARGPLRPEAAELVARIEAPVVAVDLPSGVDPDTGAVDGPHVTAARTVTFGALKPVHVLAPEECGEVVLVDIGLRPWLDEPDLHRLDLADVAEVWPIPGPEDDKYTQGVAGIAAGSATYPGAAVLASGSAVRATAGMVRYAGHAADVVRSRWPEIVATGSVTDAGRVQAWVVGPGIGTGGEGREILRHILGQGVPVCADADATTIIANTPEVLDARDPDTPLVLTPHAGEYERLMGRRPGADRVAAAREAAKKYDAVVLLKGHCTVVAAPDGRVAVNTPRGSWLATAGSGDVLTGLVGSLLAAGLDPWLAAAAAAQVHSLAGALAAEDVPTSASGLVDAIPAALRLIRATA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.2. The protein have enhanced (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.3. The designed protein should be optimized for metal ion binding, ADP-dependent NAD(P)H-hydrate dehydratase activity, kinase activity, ATP binding, NADHX epimerase activity activity in order to improve nicotinamide nucleotide metabolic process, phosphorylation.4. The (6S)-NADPHX, K(+), AMP binding site should be stable and able to withstand changes in environmental conditions.5. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.6. The protein contains novel YjeF N-terminal domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MRAMDAQTCKLHAMTGLALMERAGRAIFSKLSTTKPWCETRPLIVCGPGNNGGDGLVVAEALLLAGMDVSVCLVSEDQHLSTENRQVYERLKDRVHRIQRSEDIEWLKQQISCHDWILEALFGIGLTRPIEGVFKAVVALLNASGKSIVSIDLPAGLHTDSGQVLGMAVNATETWAIQAFKFGHFLADGKDYCGRLDCVDVGIVPVETPEQIEAGVGGALALGGSPGFTGAITLTARAALRAGAGLVTAAVPRGCQPVVAAGAPEIMTWGLPETAGGSLGRSALDPLLERLENFSVLAIGPGLGRDPETVELVKSLLPRASIPLVVDADALNALATDTGILSQPHGPMVLTPHPGEMGRLIGCPAAKIQEDRLNIARRYAQEWRCVLLLKGARTVVACPDGRAYINPTGNPGMATGGSGDVLTGIIAGLIAQGMSLDKAAAIGAYLHGAAGDLAAEELGQEALIAGDILDEHDVLSILPLRPSDAHKGSFGKVMVVAGSTEFIGAASLVCQSAARAGAGLVTLAARQSLHPVFAAKLVETTHLPLPETAHGEFASEAAEVALARLVKYDAAAIGPGLGQSPDTVEFVHQLLSGISENTGLVLDADALNALSLTPDWWDIFNHPAVLTPHTGEFARLSGLSIGEILANRMEICRKNAALWGKVVILKGAHTVVASPDGRIAVSPSANPGLASGGTGDVLTGIITGLLAQGLDEFDAARAGVYIHAMAGESVRNNIGDTGMIASDLLIQIPRAVKSTP",
    "response#2": "MGRLDRLAESQFGIPSIVLMENAGLRVFESIKRYFHNFLAGRRVAVFCGKGNNGGDGIVVARHLLNQGSEVKVFLLGSPQDLKGDPQINLKIYQKMGGRLYPLTEENHLQRVDISLLYADLVVDAIFGTGFKGAALGLAAEVIQRINGSDKPVVAVDLPSGLEADTGKVWGPCVRARWTVTFALPKLGLVVEPGASYAGALEIADIGIPYKLVPDGHKYARGHVLVVGGGAMTGAARLAARAARRIGAGLVTIAAPAEALVTYRAGDPGAIVIDAEPFDAVLADTRRNAVVIGPGGGVGEGLADRTLAALAAGRACVVDADALTSFSEDPDRLFKALSASCVLTPHDGEFTRLFGAATGSRLERAQEGARRSGAVLLLKGPDTVVAHPDGRATITTDASPALATAGSGDVLAGLIAGLIAQGMPAYEAASAAAWLHGQAGAAVGPGLISEDLSEALVGCTGPITDPTPGARSDKYSGGVVALAAGSDTYPGAGVLCATAAVRATPSMVRFIGDNTITSLLPELVCHPDVPSSGRAQAWVVGPGRGTDSAAATELRKVLAQGLPTVIDADALTLLATNTSLRQLVAAHPLTILTPHEGEFTRLYEAAFSAAPSAAWGDALHRLAEELNSIILLKGRLTRVTAPGQPIYSCNAGHSFAATPGSGDVLSGILGAVIAQLSADTPVEVITEVLHAGAIHAHAAAIAAETPDGLAPCSASQIAAAIPQAIARLLKR",
    "response#3": "MGQADAATIASGVPGIELMRRAGQAVALAVARRWQPCRVAVLCGPGNNGGDGFVAAQWLRGRGWPVRVFLLGQMERLRGDAAWAASQWQGVTEEAAAFDPAQCDIAIDALFGAGLCRPLDGLAADLVQQLAASGLPVCAVDVPSGLDGASGQPQGPVAAAALTVSFFRAKPGHWLYPGRALCGELVLADIGIADDVPSDKYSGGVVALAAGSDTYPGAGVLCATAAVRATPSMVRFIGDNTITSLLPELVCHPDVPSSGRAQAWVVGPGRGTDSAAATELRKVLAQGLPTVIDADALTLLATNTSLRQLVAAHPLTILTPHEGEFTRLYEAAFSAAPSAAWGDALHRLAEELNSIILLKGRLTRVTAPGQPIYSCNAGHSFAATPGSGDVLSGILGAVIAAESDKYRRGVVGVVAGSARYPGAAVLAVAGALRGGAGAVRYVGPAADAVLAHFPETLVHAGPPHKAGRVQSWVIGPGIGDESDALGEVLAAEVPVLVDADGLRFLTPQQVRSRTAATLLTPHAGEAAALLSHGREEIEAARLRSVRELAARYGATVLLKGSTTLVADATGPVRVNPTGTGWLATAGSGDVLSGVTGSLLAAGLPARDAASVGAYLHGLAARRAAGSPGAPILASEVALALGGSPGFTGAITLTARAALRAGAGLVTAAVPRGCQPVVAAGAPEIMTWGLPETAGGSLGRSALDPLLERLENFSVLAIGPGLGRDPETVELVKSLLPRASIPLVVDADALNALATDTGILSQPHGPMVLTPHPGEMGRLIGCPAAKIQEDRLNIARRYAQEWRCVLLLKGARTVVACPDGRAYINPTGNPGMATGGSGDVLTGIIAGLIAQGMSLDKAAAIGAYLHGAAGDLAAEELGQEALIAGDIL"
  },
  {
    "reference": "MVEIKSYCEAGVSVQHVWVEGGLTLCFYFTLVPSVLLTLSFLFGTFLCICYRRYGTDMEPKFIPRSRLYRLQVGLSVLLVLQALGWMVFRITRSGELPGYVVLSGCLSMLGWIWAMALLHLERRRVLVRDRTRGHSTVLLLYWAVAFAAENLAFVSWMSPQWWWTLEKSDQQVRYVCSLGFEPTTFLNQLTDGSSWKTLATTVCVYGLLKFLQGGGAGASGFVSNMRSFLWIRVQQYTNRMVQVRLFAHLHSLSLRWHLGRRTGDVLRSIDRGTSSINNLLSYIVFSIFPTIADIVIAIVYFISNFNAWFGLIVFVCMTLYLTLTIVITEWRTKYRRDMNTQDNNAKSKAVDSLLNFETVKYYNAEVYEVRRFEDAIMKYQVSEWKTNASLAFLNQTQNLIIGLGLLTGSLLCAYFVTEGKFQFTLSKNIFILVEILKDVSFTVLPGQIVALVGQSGSGKSTIIRLIFRFYDVQGGCIKIDDQDISKVKQSSLRAHIGVVPQDTVLFNDNIRDNIRYGRVTATDQEVEEAATAADIHDKIITFPDGYDTQVGERGLKLSGGEKQRVAIARTILKAPQIILLDEATSSLDTQTERNIQASLAKVCTNRTTIVVAHRLSTVIGADVILVLRDGQIVERGRHEELLAKGGLYSDMWLKQQQAQDSDSASDTETKDRKSEKLQPQTSTAGHKEH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should exhibit Basic and acidic residues compositional bias.2. The protein contains novel ABC transmembrane type-1, ABC transporter domains that confer a unique function or activity.3. The protein should have a Helical domain that is long enough to span the entire membrane.4. The protein can be targeted to melanosome membrane, extracellular region, lysosomal membrane, Golgi membrane and perform ATP binding. The designed protein sequence is ",
    "response#1": "PGKTSAIVGHSGGGKSTIVRLLFRFYDVNSGSIMIDGENIKLVTQESLRRAIGVVPQDTVLFNNTVFYNIEYGRLGASKEQVFEAAKSADIHERILSFPDRYDTTVGERGLRLSGGEKQRIAIARTILKSPSIVLLDEATSALDTQTERNIQSALQRVCANRTTIIIAHRLSL",
    "response#2": "IFYGILLYLGEVTKAVQPLLLGRIIASYDPDNQMERSIAIYLGIGLCLLFIVRTLLLHPAIFGLHRIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNNLNKFDEGLALAHFVWIAPLQVALLMGLLWDLLQFSAFCGLGLLIILVVLQAILGKMMVKYRDQRAAKINERLVITSEIIDNIHSVKAYCWESAMEKIIKNLREVELKLTRKAAYMRFLTSSAFFFSGFFVVFLSVLPYTVINGIL",
    "response#3": "KGEMLAITGSTGAGKTSLLMMILGELEASEGKIRHSGRISFCSQFSWIMPGTIKENIIFGVSYDEYRYKSIVKACQLQEDISKFAGKDHTVLGEGGVTLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEEQIFENCVCKLMANKTRILVTSKMEHLKKADKILILHEGC"
  },
  {
    "reference": "GSGADCFWKYCV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Urotensin is found in the teleost caudal neurosecretory system. The designed protein sequence is ",
    "response#1": "PPISIDLTFHLLRNMIEMARIENEREQAGLNRKYLDEC",
    "response#2": "PPISIDLTFHLLRNML",
    "response#3": "DDPPISIDLTFHLLRNMIEMARNENQREQAGLNRKYLDEV"
  },
  {
    "reference": "MLRTPEQRPRVGGCGPPRPKPLTSFLFQGYFAGLPGWPGETETRLLSVPKREPRWEPQAGSGGADPQEDEASPRPRAAPGEAESPAETEPDRHLETYLLDCENTSGTSPSLPQAPKQPQKRSRAAFSHSQVIELERKFSHQKYLSAPERAHLAKNLKLTETQVKIWFQNRRYKTKRKQLTSDLGGLEKHASLPALKDEGFSRAPLISMYSSYPHYPYLYCLGGWSPAFW",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein have Homeobox domains with specific sequence motifs that are crucial for its activity.2. The designed protein should be involved in cellular response to xenobiotic stimulus, regulation of protein localization, androgen receptor signaling pathway, positive regulation of gene expression, negative regulation of DNA-templated transcription by localizing to cytosol, nucleoplasm and exhibiting transcription regulator inhibitor activity, protein self-association, histone deacetylase binding, cysteine-type endopeptidase activator activity involved in apoptotic process.3. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.4. The Homeobox domain within the designed protein should exhibit high affinity and specificity towards its target DNA sequence. The designed protein sequence is ",
    "response#1": "MIPVFCVVEQLDGSLEYDNREEHAEFVLVRKDVLFSQLVETALLALGYSHSSAAQAR",
    "response#2": "MIPVFCVVEQLDGSLEYDNREEHAEFVLVRKDVLFSQLVETALLALGYSHSSAAQATTGGLAKITGLSLTQVSNWFKNRRQRDGLMIPVFCVVEQLDGSLEYDNREEHAEFVLVRKDVLFSQLVETALLALGYSHSSAAQAQVERVERENLSDYCVLGQRPMHLPNMNQLASLGKTNEQSPHSQIHHSTPIRNQVPAVAAAAADNRRDIGDILQQIMTITDQSLDEAQAKKHALNCHRMKPALFSVLCEIKEKTGLSMRNVQEEEPPDPQLVRLDNMLLAEGVAGPEKGGGAAAAVSAATSSGGMSPDSSLEHSDYKSKLSQIRNIYHTELEKYEQACSEFTTHVMNLLREQSRTRPVSPREIERMVAIIHRKFSSIQTQLKQSTCEAVMILRSRFLX",
    "response#3": "MIPVFCVVEQLDGSLEYDNREEHAEFVLVRKDVLFSQLVETALLALGYSHSSAAQALSDIGDILQQIMTITDQSLDEAQAKKHALNCHRMKPALFSVLCEIKEKTGLSMRNVQEEEPPDPQLVRLDNMLLAEGVAGPEKGGGAAAAVSAATSSGGMSPDSSLEHSDYKSKLSQIRNIYHTELEKYEQACSEFTTHVMNLLREQSRTRPVSPREIERMVAIIHRKFSSIQTQLKQSTCEAVMILRSRFLN"
  },
  {
    "reference": "MNQGTSFRHLLLLLQLALLLGATQGREVVLGKAGGQVELPCKASEKKDMPFTWKDSRGNTIVKTYGQFWYMCSSKLKDRVHSPKSLWNQGSFPLIISNLEITDSGIYSCTVEQQTEVELVVFKLNANLDIKNRIHLLQGQSLTLTLESPPGRNPSVEWKGPGNKELKRGKSLSLSQVESQDRGTWTCTVSQDTKKLVFEINILVLAFRDVSDTVYTKVGEPVEFSFPLTFEDENLVGELRWQEEGTSSPQPWITFSLENRKVTVKKVRKDRKIQLKETLPLTFSLPQASLQDVGSGHLTLNLSQGQLHQKVNLAVMRMTKSQNQLTCEVLGTNTSELMLSLKLDNQIAKVSGQQKLVQVKDPEAGMWHCLLTNNNEVLLESNVEASGPKLTQASPKLLAIVLGAVGGFLIFIGFCIFCCVKCWHRRRQAVRMSQIKRLLSEKKTCQC",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Include a signal peptide as an essential component of the protein.2. A protein that can perform protein tyrosine kinase binding, coreceptor activity, protein homodimerization activity, MHC class II protein binding, zinc ion binding in external side of plasma membrane, membrane raft to support positive regulation of monocyte differentiation, maintenance of protein location in cell, regulation of calcium ion transport, adaptive immune response, cell adhesion.3. The presence of Ig-like domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MDPKGLLSLTFVLFLSLAFGASYGTGGRMMNCPKILRQLGSKVLLPLTYERINKSMNKSIHIVVTMAKSLENSVENKIVSLDPSEAGPPRYLGDRYKFYLENLTLGIRESRKEDEGWYLMTLEKC",
    "response#2": "VEVVTQDERKLLHTTASLRCSLKTTQEPLIVTWQKKKAVGPENMVTYSKAHGVVIQPTYKDRINITELGLLNTSITFWNTTLDDEGCYMCLFNMFGSGKVSGTACLTR",
    "response#3": "PEGNFHKVYDLQVLVPPEVTYFLGENRTAVCEAMAGKPAAQISWTPDGDCVTKSESHSNGTVTVRSTCHWEQNNVSAVSCIVSHSTGNQSLSIELC"
  },
  {
    "reference": "MIKTVQDLINERIAVGKVVNTHGLKGEVKFFPYTNSEEIVKNLSSVVLYNREKKVFYNLSVESVRRMNKLFLIKFETIDTVEAAEKIKGCEVFIKYEELPPLQNDEYYFYEILGCEVYYESGECVGKVVDIIETGSNDVLVVKKKNRETLIPMIKDCVVEIKKPEKKIVVKELEWI",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Essential for efficient processing of 16S rRNA; Probably interacts with S19; May be needed both before and after RbfA during the maturation of 16S rRNA; An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region; It has affinity for free ribosomal 30S subunits but not for 70S ribosomes..2. A protein that contributes to ribosomal small subunit biogenesis, rRNA processing by functioning in cytoplasm, ribosome with ribosome binding. The designed protein sequence is ",
    "response#1": "GRYYYFALIGLPVYVEGRQVGEVVDILDAGAQDVLIIRGVGERLRDRAERLVPLQAPYVRVEEGSIHVDPIPGL",
    "response#2": "MGRYYYFALIGLPVYVEGRQVGEVVDILDAGAQDVLIIRGVGERLRDRAERLVPLQAPYVRVEEGSIHVDPIPGS",
    "response#3": "MKPLILSGQYKC"
  },
  {
    "reference": "MTKRHPKTFSTIDSATEDVKQGKMIVIVDDEDRENEGDLMIAAELCTPEAINFMAKYGRGLICLTLTEQRTAQLGLPMMVPENQAQFGTPFTVSIDAKHGITTGISAADRAKTIAVAMGPESTQYDLVMPGHIFPLRAREGGVLVRAGQTEGSVDMARLAGLKPTGVICEVMNDDGTMARVPQLSKFVKKHKIKMITIKDLMEYRLNSETLVEEVASTLLPTDFGEFRVVAFRNKLIDQVHITLVKGELKPEEPTLVRVHSQCLTGDVFGSQRCDCGEQLAQALNMIEREGKGVLLYLYQEGRGIGILNKLKAYALQDAGHDTVEANAELGFKPDLRDYGIGAQILRALGLGKIRIMSNNPRKIIGLEAYGLEMVERVPIEVRPKKHNIKYLRTKQNKMGHLIKNVF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must have at least one GTP cyclohydrolase II domain.2. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..3. The D-ribulose 5-phosphate, Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.4. A key design requirement for the protein is the optimization of the Proton acceptor; for GTP cyclohydrolase activity to achieve high catalytic efficiency for GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.5. The protein should be optimized for activity in 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway and exhibit minimal cross-reactivity with other metabolic pathways.6. The protein must exhibit GTP cyclohydrolase II activity. The designed protein sequence is ",
    "response#1": "RVAEVKLPTDYGDFNAIAYESMVDKSVHMALVKGDLHSTEPMLVRVHSECLTGDVFGSKRCDCGDQLHRSMEQINAKGRGVILYMRQEGRGIGLANKIKAYALQDGGMDTVEANIELGFKADLRDYGIGAQILVDLGLTDIVLITNNPRKIVGLEGYGLKVVDRCPVFREPIRVAETKLPTQYGEFKAVAYASIIDSDEHVVLVKGDISTNDPVLVRVHSQCLTGDTFGSQRCDCGEQIHRSLEAIQAEGRGVFLYMRQEGRGIGLHNKLRAYQLQDAGLDTVEANEKLGFPADLRWYGIGAQILVDLGIKRIRLLTNNPKKVVGLDGYGLEIMERVPLMRKGENVKRVAEVKLPTDYGDFNAIAYESMVDKSVHMALVKGDLHSTEPMLVRVHSECLTGDVFGSKRCDCGDQLHRSMEQINAKGRGVILYMRQEGRGIGLANKIKAYALQDGGMDTVEANIELGFKADLRDYGIGAQILVDLGLTDIVLITNNPRKIVGLEGYGLKVVDRCPIFKE",
    "response#2": "EAVTRLPTRHGDFRAYGYRSTTDGVEHVALVAGELGDGADVLVRVHSECLTGDVFGSLRCDCGPQLQESLARVQAEGRGVVLYLRGHEGRGIGLLPKLRAYELQESGHDTLDANLALGLPADARDYGAAARILTDLGVRSLRLMTNNPDKTDALLRHGLRVEGRAHPVDLEADPRVAETKLPTQYGEFKAVAYASIIDSDEHVVLVKGDISTNDPVLVRVHSQCLTGDTFGSQRCDCGEQIHRSLEAIQAEGRGVFLYMRQEGRGIGLHNKLRAYQLQDAGLDTVEANEKLGFPADLRWYGIGAQILVDLGIKRIRLLTNNPKKVVGLDGYGLEIMERVKEAEAQLPTRFGDFKIKVYKSIIGNQEHIALVKGEVAGKKNVLVRVHSECLTGDTFGSLRCDCGQQLAAALKMIDKEGHGVLLYMRQEGRGIGLLNKIKAYALQDKGMDTVEANLALGFPPDLRDYGIGAQILADLGLTSIRLLTNNPRKIIGLEGYGLMVVDRVPA",
    "response#3": "RVSEGRIPTKYGDFKAIAFESLLDGVEHVAFVMGDPAESEDVLVRVHSECLTGDIFASMRCDCGAQLDGAMAKIGEAGTGIVVYLRGQEGRGIGLGHKLRAYELQDGGLDTVDANTELGLPVDSREYGIGAQILVDLGVKSMKLMTNNPSKFGGLAGYDFEITGRTPNADEPEAVTRLPTRHGDFRAYGYRSTTDGVEHVALVAGELGDGADVLVRVHSECLTGDVFGSLRCDCGPQLQESLARVQAEGRGVVLYLRGHEGRGIGLLPKLRAYELQESGHDTLDANLALGLPADARDYGAAARILTDLGVRSLRLMTNNPDKTDALLRHGLRVEGREAVTRLPTRHGDFRAYGYRSTTDGVEHVALVAGELGDGADVLVRVHSECLTGDVFGSLRCDCGPQLQESLARVQAEGRGVVLYLRGHEGRGIGLLPKLRAYELQESGHDTLDANLALGLPADARDYGAAARILTDLGVRSLRLMTNNPDKTDALLRHGLRVEGRTTNRPAADKVVETSIPTAHAGLFKVIVYENDLEDHQHIALIKGEIDPEKPILVRVHSECLTGDIFHSLRCDCGDQLAKAMEMMDKEGSGVLLYVRQEGRGIGLVNKLKAYVLQDKGYDTVEANEKLGFKADLRDYGIGAQVLADLGVKQMRLITNNPKKIVGLEGYGLSIVEQVTR"
  },
  {
    "reference": "MVTMNRNKVVIVGTGQVGATAAFGIVTHGLCNELVLIDCSAAKALGEARDLDDGSEFQDRHVKVRAGDYADCKDADIVVITVGRKPPANSNRMAELGFTVGLVGEVVDNVMASGFDGVIVMVSNPVDVMAWYAWKRSGLPRTQVLGTGTALDTSRLKTIIGEETGLDPRNVGGFVMGEHGDSQFTAWSTVSLGGKPFARFLADNQDRFASVSTTEIEEKTRTRGNEIVAAKGGTNFGIASTVAGIVQTILWDERRIVPVSTLLDGEYGEHDVFLGVPTELRANGANEIVELDLSEDERAKLHHSAELVREHCEGLL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be involved in glycolytic process by localizing to cytoplasm and exhibiting L-lactate dehydrogenase activity.2. A protein that is capable of catalyzing reactions within the Fermentation, (S)-lactate from pyruvate: step 1/1 pathway with high efficiency and specificity.3. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for (S)-lactate + NAD(+) = H(+) + NADH + pyruvate.4. The designed protein should have a binding site that is specific to the target NAD(+), substrate ligand.5. For general function, the protein need meet that Catalyzes the conversion of lactate to pyruvate. The designed protein sequence is ",
    "response#1": "MTSLDSARLRVGLSKLFNVSPVDVNANMMAEHGDTEFAAFSSATIGGLPLYDLAEAKGISKDDLYKLEDDVRNKAYAIINSKGATFYGVATALMRISRAILRDENAVLPVGAPMSGEYGLKDIYIGTPAVINANGIAEVLEVPLDEREAKAMADSAKTLEEIAKNGMAEVGQ",
    "response#2": "TSLDSARFRKDIAEMVNVDARSVHAYIMGEHGDTEFPVWSHANIGGIKISEWVKAHPEVKEEELVKIFESVRDAAYTIINLKGATFYGIGTALARITKAILDDENAVLPLSVFMDGQYGLNDIFIGSPAVINRSGITNILEIPLTDHEMESMHKSAKQLKDIVTKAFEEM",
    "response#3": "TSLDSSRLRVALGKQFNVDPRSVDAYIMGEHGDSEFAAYSTATIGTRPVRDVAKEQGVSDDDLAKLEDGVRNKAYDIINLKGATFYGIGTALMRISKAILRDENAVLPVGAYMDGQYGLNDIYIGTPAIIGGTGLKQIIESPLSADELKKMQDSAATLKKVLNDGLAAAK"
  },
  {
    "reference": "MDLLVATRNPGKLRELHQLLSGLPIRALSLADAGITADVAETGRTFEENAVLKAHSYAALSGLTTLADDSGLEVDALGGAPGVQSARWAGPHASDADRIRLLLERLRGVPPEQRGAQFHCTVAIATPSGAVYTTEGIIRGQIIDEPRGSHGFGYDPVFFIPERGRTMAELEPEEKNRISHRARALQAARPILARLAAESEGG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have a low Km value for dITP + H2O = dIMP + diphosphate + H(+).2. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP3. The protein must bind to Mg(2+) in order to perform its enzymatic function.4. The protein should have a high affinity for substrate.5. The protein design must be able to perform metal ion binding, XTP diphosphatase activity, ribonucleoside triphosphate phosphatase activity, nucleotide binding in order to contribute to nucleotide metabolic process, purine nucleoside triphosphate catabolic process. The designed protein sequence is ",
    "response#1": "MAIALVTGANKGIGFEVARQLGADGWQVVVGARDEARGQAAVARLGPSARVVPLDLTDLASVEAFAAEVGATDRLDVLVNNAGVMRPTAPRDARGVELTFATNVTGPFVLTQRLLPLLARSAQPRVVLTSSGAGSLTRMDLQDLQGEFSFSGFKAYATSKLANILFTVELARRLEGTGVTAYCLHPGIVRTNLGADAGGWLAPLFSRVAKPFLQSAEDGALPILRVATAPELEGVTGRYFGADGPTEVSARARDRALADRLWELSETLVGPRVKG",
    "response#2": "MXTQTIVLATRNAHKARELSLVLGRSVVGVDVLPGAADVVEDGTTFAENALIKARAVAAATGLPALADDSGLSVDALGGAPGIYSARFAGEHGNDAANNAKLLAELADIPDGQRGAHFVSALCLAWPDGHATIVEGRMRGTITTAPRGDGGFGYDPIFIPDGADGGLTTAQLTPAEKDAASHRGQAFRALVPVVADLLA",
    "response#3": "MIVLASNNQGKIKELQALLAPFQIEVLPQSAFNISEAEEPHCTFVDNALAKARHASALTKHPALADDSGLVVPYLGGAPGVWSARYAGEHGDDAANNAKLLHELADVPEAERSARFQCVIALVVADGEPAIATGIWPGRILAEANGENGFGYDPYFYLPELNATAAQLPMDVKNRLSHRAQALQQLLAKLP"
  },
  {
    "reference": "MAAVDSDIEPLPRGGFRCCLCHITTANQPSLDAHLGGRKHRHLVELRATRKAQGLRSVFVSGFPRDVDSTQLSEYFQAFGPVASVVMDKDKGVFAIVEMGDLGAREAVLSQPQHSLGGRRLRVRPREQIEFQSPASRSPKRVAPDSHQLIKALAEAPDVEAQMVKLVGLRELSEAERQLRSLVVALMQEVFAEFFPGCVVHPFGSSINSFDVHGCDLDLFLDLGDLDEPQPAPKAPESPSLDSALASPLDPQALACTPASPPDSQPPASPQDSEALDFEAPSSSLAPRTPDSALASETLASPRSLPPASPLQEDQGDGDQGKAVELAEALKGEKAEGGAMLELVGSILRGCVPGVYRVQTVPSARCPVVKFCHRPSGLHGDISLSNRLALHNSRFLSLCSELDGRVRPLVYTLRCWAQGRGLSGSGPLLNNYALTLLVIYFLQTRDPPVLPTVSQLTQKAGEQVEVDGWDCSFPRDASRLEPSTNKEPLSSLLAQFFSCVSCWDLRGSLLSLREGQALSVAGGLPSNLSEGLRLGPMNLQDPFDLSHNVAANVTSRVAGRLQNCCRAAANYCRSLQYQRRSSRGRDWGLLPLLQPSSPSSILSATPIPLPPASFTQLTAVLAQVLREALGCHIEQGTKRLRSEGGGPGEPPQGGTSKRAKLDGQKKSCEEGPEEQQGCAGEHGEDGVEEMVIEVGESVQDWVMRSPGQLGELPLMTGKHLATREEGQSGTAALAKQGPRGPEAACEGSQAEAEKRVSLTVSWRCALWH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should exhibit Polar residues compositional bias.2. The designed protein should contain one or more RRM, PAP-associated domains that are essential for its function.3. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP, Mg(2+) binding.4. We need a protein that can perform ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide with high efficiency.5. The designed protein should be involved in snRNA processing, mRNA polyadenylation by localizing to nuclear speck and exhibiting ATP binding, polynucleotide adenylyltransferase activity, U6 snRNA binding. The designed protein sequence is ",
    "response#1": "MLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRAVMVEEFKQGLAITDEILLVKAEWSKLFR",
    "response#2": "NPFAQSPEAADALEALGINSHPYAITSHTHAAAKAIESDLYEVVSHYLPKENPVSFLFMKPAKLRFFHRGPTHGDHFLNAHVEPKDVPRYPQETIINRLADIPTQIAFMGDTLHFLSPSFLTALFAHSPRLQTLYATLVLPPEPSTSRSLYPQIYTLTYSEDGFMYIPGGHAGASYFHKYDQLEWLTVGHLTAPGCPTVTAQRLETKGANHLFIFQRGNYITPERRTFATNDQYVTVPHIFLPENYNCRTPISKTTMMQMFFYIKSGKEAQREGHLGQVQQLQMVKLVGLRELSEAERQLRSLVVSLMQEVFTEFFPGCVVHPFGSSINSFDVHGCDLDLFLDLGRHPYAITSHTHAAAKAIESDLYEVVSHYLPKENPVSFLFMKPAKLRFFHRGPTHGDHFLNAHVEPKDVPRYPQETIINRLADIPTQIAFMGDTLHFLSPSFLTALFAHSPRLQTLYATLVLPPEPSTSRSLYPQIYTLTYSEDGFMYIPGGHAGASYFHKYDQLEWYNP",
    "response#3": "EQQIKKLVSLCSPSHHESHLRELLLSLLQETFTEFFPGCQLLPFGSSVNGFEISGCDLDLYLDLGDDEAENVEGKAEKEIQNREESSTDMEVSMEDPETERKEEEMEIGNSKNDEDEDVTPGLSLKGLSSEEILEVVGKVLRHCVPGVHGVQSVPTARRPVIHFQHKTSGLRGDVTLNNRLALRNSSFLRK"
  },
  {
    "reference": "ECKYLWGTCEKDEHCCEHLGCNKKHGWCGWDGTFG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: In vivo, reversibly paralyzes cockroaches for several hours, paralyzes rat after intracerebroventricular injection and blocks the neuromuscular transmission of the isolated rat phrenic nerve-diaphragm preparation (Ref.2).; [Hainantoxin-VI]: Gating-modifier toxin that dose-dependently inhibits inactivation of voltage-gated sodium channels and reduces the peak of sodium current in cockroach DUM neurons. The designed protein sequence is ",
    "response#1": "LCLGWFKSCDPKNDKCCKNYSCSRRDRWC",
    "response#2": "GCLGWFKSCDPKNDKCCKNYSCSRRDRWC",
    "response#3": "GCLGWFKSCDPKNDKCCKNYSCSRRDRWC"
  },
  {
    "reference": "MDTDIGSRTWHAHAKINLALHVTGRRADGYHLIDSLAVFTRFGDRVEIALADSDDFTVSGRYAPAVPLDAGNLVLKARDALRREAGSGRTPPVSIKLEKNLPIASGVGGGSSDAAAVLRGLAQTWALDIDDAGLARIGLSLGADVPMCLAAKPLVARGIGDELSMVPDFSALGLVLVNPGTPVSTADVFAALSRRDNEPLPPLPRSIDFHSLRNWLEVTRNDLEPAALALQPAIGRALSWLNKAGSGFSRMSGSGATCFGLFETGNVAKRAAAEIRSRQPDWFVAATRSMSSEAE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should interact with enzymes and substrates within the isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 3/6 pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MNPPSMLFPIQHVLTTPKRCPAPHKGMDFSIKQILEFFPIDEERFPSIALARQVLQDKGSSGPFFNAANEILVQRFLKKEIAWCDILDKLTRLMKNHRVSSCTSLDDVFSVDKEARR",
    "response#2": "MRVGLGYDVHKLGYNTPLILGGVNIPFNKGLIGHSDADVLVHAIMDALLGALSLGDIGKHFPPSDMKYKNISSLKLLNYVSNLIKSKGYSIGNIDSTIIAEEPKLFPYIPDMRLNIAKILKVNTSIISVKATTEEGLGFTGKKEGIAAQSICLLS",
    "response#3": "MNPPSMLFPIQHVLTTPKRCPAPHKGMDFSIKQILEFFPIDEERFPSIALARQVLQDKGSSGPFFNAANEILVQRFLKKEIAWCDILDKLTRLMKNHRVSSCTSLDDVFSVDKEARR"
  },
  {
    "reference": "MEKLSFELALKKLEESVEQLESGELELEESIKVFEQGIELSLFCRKELSQAEGKIQRLVKNLGGEFELLDFEV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Bidirectionally degrades single-stranded DNA into large acid- insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.2. The designed protein must be able to regulate DNA catabolic process. The designed protein sequence is ",
    "response#1": "LFDEEKKKPLPYMPKIIGVVTSPTGAVIRDIIHRISDRFPLHILVWPVRVQGETSGREVAAAVKGFNVLPLEGLIPKPDLLIVARGGGSLEDLWGFNDEVVVRAVYESSLPVISAVGHETDWTLIDYVADWRAPTPTAAAEKAVPVKIDLEVYVVSLGARLRTGLARYFDFHQQKLRAIIRALPTADQLFALPRRGFDEISSRLERALCVSCDKKRLYFHTLAIRFTPRLLNTEKAQHSAKEYTARLHRAFVHIVEKQRAQLEIAFRLLKSTSYQNILERGFVLALGQNNKPIKRLAQIPEKEQINLRFFDGEISR",
    "response#2": "APKLPIPKYPRKIAIVTSPSGAVIRDIITTVSRRYPIVQLVLFPALVQGNEAANSIAAQIKMINTLDDFDTIIIGRGGGSIEDLWPFNEEVVARAIFASKLPVISSVGHETDTTIADLVADMRAATPTAAAELATPVLTDILEELQKLQLQTIVAFRNILKMRSQQVQHLQQSYIFQEPQRLYEGYVQNVDILTEKLISLEKQQITTAEGSFKTLNSRLLANTPASRIKMAKQNVEHLRQMTNNNITNRFSKYANDLNSLIGSLDTLSPLKIMSRGYTYVTRDTKVVKSIEDLSIDDKIQLNFSDGSANQ",
    "response#3": "APKLPIPKYPRKIAIVTSPSGAVIRDIITTVSRRYPIVQLVLFPALVQGNEAANSIAAQIKMINTLDDFDTIIIGRGGGSIEDLWPFNEEVVARAIFASKLPVISSVGHETDTTIADLVADMRAATPTAAAELATPVLTDILEELQKLQLQTIVAFRNILKMRSQQVQHLQQSYIFQEPQRLYEGYVQNVDILTEKLISLEKQQITTAEGSFKTLNSRLLANTPASRIKMAKQNVEHLRQMTNNNITNRFSKYANDLNSLIGSLDTLSPLKIMSRGYTYVTRDTKVVKSIEDLSIDDKIQLNFSDGSANS"
  },
  {
    "reference": "MAHMRDLKRRIRSVQSTQHITRAMKMVAAAKLRKAQAQVTAARPYAAKLEEVVGRLMAAVDPETQPLAATREVKKAGYVLITADRGLAGGYNANLIRLTEERLREEGRPAALVAVGRKGRDFFRRRPVEIVKSFTDIGDNPELIQARELARQLVTMYLEGTLDEVNLIYTRFYSAIRQVPMVERLLPIATPREKKDTGDYIYEPSPEAVLRVLLPRYCEIKVYRALLEAKASEHGARMTAMDNATKNAAEMIDKFTLSFNRARQAAITNEIVEIVAGADALK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Produces ATP from ADP in the presence of a proton gradient across the membrane; The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex..2. The designed protein should have proton-transporting ATP synthase activity, rotational mechanism within plasma membrane to regulate proton motive force-driven plasma membrane ATP synthesis. The designed protein sequence is ",
    "response#1": "EMPK",
    "response#2": "FFSNGL",
    "response#3": "GLLPHF"
  },
  {
    "reference": "MFLEKDQDNLDNLETQAKELHKDCKECKNCQKEETKTTNKDNQKEDETVKNQSNQSNQSNQTKQTNTKQQKHQPKENSHLQITKLQTQIKELQQQLTQQKKSFDEELLKNQAELINFKKRAQTQKANELKYASSNFITNLLMPLEQLEKVIDMPTQNELLQKYLLGFKLLQQQIKKVLQDEGVEEIEALNKPFDPALHHALETVCDPKKPDKTNLAVLQKGYLYKKRILRPTLVKVNEWSDKNDKNE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex; It is the nucleotide exchange factor for DnaK and may function as a thermosensor; GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle.2. The protein should exhibit Polar residues, Basic and acidic residues compositional bias.3. The protein must have high specificity towards protein homodimerization activity. The designed protein sequence is ",
    "response#1": "GIKKEIRIPKLDKCQSCYGYGSAYGSKPSTCTSCNGHGQIHMRKGFFSVQQTCSTCRGTGTMIKNPCKICFGQGRIKKC",
    "response#2": "GGSQVVKIDSLVVCDVCNGTRSEPGYKAEVCFDCNGSGVVRGEVRTTLGNLITQNTCSKCRGNGERIDHPCRRCYGNGSRSAC",
    "response#3": "GGSQVVKIDSLVVCDVCNGTRSEPGYKAEVCFDCNGSGVVRGEVRTTLGNLITQNTCSKCRGNGERIDHPCRRCYGNGSRSAC"
  },
  {
    "reference": "MINFSLSLTKSMSYSFIWMLFVIHISCVLSADGNHILCSPSFTCGNQRGLLYPFWIAGRKECGHPDFELDCNAGVPELSISSVKFRILGADYDSGIITLARSDNIDDPCLPNSFTTSFNETVLPLASTTDLLTIYYDCNRNVSSFVSTFVKELDCPDDGTDDRRNYYLTRNLTFLPPSLKLEGNSFLLNDFGGSCSRNVSNPASRTALNTLESTPSTDNLKIALEDGFALEVNSDCRTCIDSKGACGFSQTSSRFVCYYRQEPQNPTRNKVILKLFFIVIYVLGIGAASFAMMGVILVVTCLNCLIRRQRKTLNDPRMRTSDDSRQQNLKALIPLKHYSYAQVTSITKSFAEVIGKGGFGTVYRGTLYDGRSVAVKVLKESQGNGEDFINEVASMSQTSHVNIVTLLGFCSEGYKRAIIYEFMENGSLDKFISSKKSSTMDWRELYGIALGVARGLEYLHHGCRTRIVHFDIKPQNVLLDDNLSPKVSDFGLAKLCERKESILSLMDTRGTIGYIAPEVFSRVYGRVSHKSDVYSYGMLVLDIIGARNKTSTEDTTSSTSSMYFPEWIYRDLEKAHNGKSIETAISNEEDEIAKKMTLVGLWCIQPWPLDRPAMNRVVEMMEGNLDALEVPPRPVLQQIPTATLQESSTFSEDISAYTEICSINVA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Incorporate a signal peptide in the protein design.2. The designed protein should have a binding site that is specific to the target ATP ligand.3. The designed protein should be involved in protein phosphorylation by localizing to membrane and exhibiting polysaccharide binding, protein serine kinase activity, ATP binding, protein serine/threonine kinase activity.4. The designed protein should have at least Helical transmembrane domains to ensure proper localization.5. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-   threonyl-[protein].6. The designed protein should contain Extracellular, Cytoplasmic topological domains.7. The designed protein must have at least one Protein kinase domain. The designed protein sequence is ",
    "response#1": "MNRVLGQGGQGTVYKGMLAEGRIVAVKRSKVVGEGKMEEFINEVVLLSQINHRNIVKLLGCCLETEVPVLVYEYIPNGDLFKRLHEKSESNDYTMTWEVRLRIAIEIAGALSYMHSAASIPIYHRDIKTTNILLDEKYRAKVSDFGTSRSITIAQTHLTTLVAGTFGYMDPEYFLSSQYTDKSDVYSFGVVLVELITGEKPLSY",
    "response#2": "FDKDGRLGKGGFGEVYRGNLPHVGDIAVKRVCHDAKQGMKQFVAEVVTMGSLKHRNLVPLLGYCRRKGELLLVSEYMSNGSLDQYLFHREKPALSWSQRLVILKDIASALSYLHTGANQVVLHRDIKASNVMLDSEFNGRLGDFGMARFEDYGDSVPVTAAVGTMGYMAPELTTMGTSTRTDVYAFGVLMLEVTCGRRPLDPKIPSEKRHLIKWVCDCWRRDSIVDAIDTRLGGQYSVEETVMVLKLGLICTNIVAESRPTMEQVIQYLEQL",
    "response#3": "LGLKGELKLADFGWSVHAPSLRRTTFCGTTDYLAPEMIDGRTHDENVDVWCVGVLCFECLVGYAPFEAPTRNETYKRIAELIGACRTKTYVQEHSVVDPVKKTMELKSSNISFTNLVSVDERLIYKPHPQEPEKTILTQEAIISVKGVSVSSYLEGLMENTISSNANKGREALEWVISKLNAEIL"
  },
  {
    "reference": "MMKEKNIIVGVTGGIAAYKSCELVRGLIKKDVSVQVVMTKNATEFITPLTLQTLSGNKVAINTFDLEWESEIGHITLADNADLIVIAPATANFIGKLASGIADSLLSTIVLATKAPIVLCPAMNVNMYENPAVQENIAKLRERGFIIMEPEEGNLACGWEGKGRLPEIEVILGEIEKTLTPDDMSKEKVLVTAGATREFIDSVRYISNPSSGKMGYALAREALNRGAEVVLISGKSSLCPIPGVKTINIESSDEMYKSVMEHLDWSTVVIKAAAVGDYTPTKLHNGKIKKDDKNKILELKRTKDILHDIGKKKNGRIVIGFAAETDNLIKNAKEKLKKKNVDLIVANDVSQSGAGFEEDTNIAHLIYGNSVEELPLMPKSLLARKIFDKISDIKKDQKNN",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should consider the endogenous proteins and enzymes present in the CoA from (R)- pantothenate: step 3/5 pathway to avoid interference with native metabolic processes.2. The protein should be able to bind CTP ligand in a variety of conditions3. Mg(2+) is required for the proper folding and stability of the protein.4. The design of the protein must focus on the precise arrangement of amino acids in the Proton donor to enable effective catalysis of H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.5. The designed protein must possess FMN binding, metal ion binding to support coenzyme A biosynthetic process, pantothenate catabolic process under certain conditions.6. The designed protein should contain one or more Flavoprotein domains that are essential for its function.7. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine. The designed protein sequence is ",
    "response#1": "MNIVLGITGGIAAYKAPDLVRRLKERGGDVQIVMTVSAAEFVTDTALQAVSGRPVRSNLWDKEAERAMSHIELARWADVILIAPATAEVMARIVSGGAPDLLTTICLATEAPIAVAPAMNHIMWSNPATQANRKVLEERGIHILGPGVGSQACGETGAGRMLEPDTIAAAVFEALEDDASGMLTGRRIIVTSGPTHEPIDPVRYIANRSSGAQGTAIARALAALGAEVVFVTGPADVPPPVGVEVMRVETAREMLAAVESALPADAGVFAAAVADWRVKEASMSKIKKEKGTLPSLSFEENPDILATVCRSEQRPRLVVGFAAETDDVVSHATAKRARKECDWIVANDVSPGTGIMGGSENAITLLRVEDGDVEAWPTMSKRDVAERLVGRVADAL",
    "response#2": "MNIVLGITGGIAAYKAPDLVRRLKERGGDVQIVMTVSAAEFVTDTALQAVSGRPVRSNLWDKEAERAMSHIELARWADVILIAPATAEVMARIVSGGAPDLLTTICLATEAPIAVAPAMNHIMWSNPATQANRKVLEERGIHILGPGVGSQACGETGAGRMLEPDTIAAAVFEGRRVVITAGPTHEPIDPVRFIGNHSTGRMGIALAEEFARRGAEVDLILGPSSLKPSEAVRTTRVTTAEEMLQATEEVIEEADIAIFSAAVADYRPRYHYTEKIKREGQAEMELELVRNPDIAATLGARKRPEQFFVGFALESNAGVEEAKRKMHAKHFDAIVLNSLQDAGAGFAHETNKVTILYADGSTTELPIMTKEQVADAVWDAVVGLL",
    "response#3": "MNIVLGITGGIAAYKAPDLVRRLKERGGDVQIVMTVSAAEFVTDTALQAVSGRPVRSNLWDKEAERAMSHIELARWADVILIAPATAEVMARIVSGGAPDLLTTICLATEAPIAVAPAMNHIMWSNPATQANRKVLEERGIHILGPGVGSQACGETGAGRMLEPDTIAAAVFRVLLGVTGGIAAYKVCTLASHLTQAGARVDVVMTEAATRFVTPLTFQSLTGRPVYTSLWGAMESDAHGGESALLPTHVVHVGLAEAAELMVIAPATANTLAKLSHGIADNLLTSLALAARCPVLVAPAMDGGMWANAATQENVATIRERGVHVVGPAIGRLASGLEGEGRMAEPEEILGHIRRALVAALAPELTGRRIIVTSGPTHEPIDPVRYIANRSSGAQGTAIARALAALGAEVVFVTGPADVPPPVGVEVMRVETAREMLAAVESALPADAGVFAAAVADWRVKEASMSKIKKEKGTLPSLSFEENPDILATVCRSEQRPRLVVGFAAETDDVVSHATAKRARKECDWIVANDVSPGTGIMGGSENAITLRVVWRDGREELSGKHIIVTSGPTHEAIDPVRYIANRSSGAQGSAIAAALRDLGASVSFVTGPASVPPPQGVTVIRVETAAQMAAAVAAALPADAAVMAAAVADWRVANASDSKMKKDGTGQAPALQFIENPDILRDTSASKQRPKLVIGFAAETDDVLSHAAAKLARKGCDWIVANDVSPATGIMGGAENAVTIELIGSDGRITELALGDKRDVADAVWDLVAERLR"
  },
  {
    "reference": "MFESLYTAAEMRAAEERYPGFPTTASELMERAGVAVAQEALRAFPDARRFAVVCGGGSNGGDGRIAARELRAAGRDAVETSAVERADVIVDALFGTGFHGKPRAEATALIERMNSAGGSVVAVDLPSGIDASTGEAAGAAVRANLTVTFHGCKLGLVVAPGRFHAGEVVTVDIGLDDTETEAALVTPAILDLLPRKQVQDNKYSAGSVLIVGGAPGLTGAVCLAAEAAFRADAGYVAVAVPEASLPIVETKLLEPVKLMWAATFEAAGKAGAVAVGPGLGRTDEAKAIVGRILAELEQPVVLDADALYELEPGDWRPPVVITPHVGELGRLLGESSDWVSDHRLEAARRAAARFGCVCLLKGADTIVAAPEARTLVCRIDAPALATAGTGDVLTGVLAAFLAKGLEPRLAAAAAAIATAEAAKRVPYRTGVVAGDVVAALPQPHD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit ADP-dependent NAD(P)H-hydrate dehydratase activity, NADHX epimerase activity.2. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration The designed protein sequence is ",
    "response#1": "LIAAGTKRMSGAGLLCAKAALRAGCGLATWAVPASLATHLLGRVPEAMLAGLADAGHGDWTHVNAEAILALAEDCDALALGPGMGRYEGDSQWLRGLWEGAGCPLVLDADALNMLAEAPDFADWQPRRAATVLTPHPGEMARLAGVSTREVQRDRIGLARRYAEQHGVTLVLKGARTVVATPDGAVYVNVNGNAGMATAGAGDVLAGIIASLLSQGFDAAQAACLGVYMHGAAGDRAALKRTSPASLLAGDLA",
    "response#2": "IAAGSATYPGAAVLATGAAVLATSGMVRYAGPAADVVRSRWPEVVATGAVTDAGRAQAWVAGPGIGTGHEGRQVLRHVLGLGVPVCADADAITILASNPEVLDARDPGTPLVLTPHDREFARIAGAEPGADRVTAARELASRVDAVVLLKGHSTIVAAPDGRALVNVPEGSWLATAGTGDVLAGLVGALLAAGLDPWLACGAAAYVHSLSARLAAEGAA",
    "response#3": "LAGSPDMPGAAILASWAALTSGAGIVRLIHQEGMNAELSAAPLELVRQTYSPQDLEPVIEVMNKSSASFIGPGMGRTPDAFLVLKTLFKYLSKPCVIDADALTMLSEKEVPLPPSAVMTPHHGEMLRLLGKDKXYFSTEQLLQLAKNFAXRKKITLVLKGAPTFILQKGHPIYVNPRGSPGMATAGSGDVLTGMIAAFXAQHLQPIQAAIVAVYLHAVAGEYAALXKTEHCLIASDII"
  },
  {
    "reference": "MDPVDPNLEPWNHPGSQPKTACNKCYCKKCCWHCQICFLKKGLGISSGRKKRRHRRGTPQSSTDHQNPISKQPLSHTQRDQTGPEESKKKVESKAEPDRFA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design must feature the Nuclear localization signal, RNA-binding (TAR), and protein transduction motif.2. For general function, the protein need meet that Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to promote processive transcription elongation by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts; This binding increases Tat's affinity for a hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA) and allows Tat/P-TEFb complex to bind cooperatively to TAR RNA; Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication; Other factors such as HTATSF1/Tat-SF1, SUPT5H/SPT5, and HTATIP2 are also important for Tat's function; The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive3. The Zn(2+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MEPVDPNLEPWNHPGSQPTTPCSKCYCKKCCWHCQLCFLKKGLGISYGRKKRKHRRGTPQSSKDHQNPVPKQPLPTTRGNPTGPKESKKEVASKAETDPCD",
    "response#2": "MEPVDPNLEPWNHPGSQPKTACNKCYCKKCCYHCQLCFITKGLGISYGRKKRRQRRRAPQGSQTHQASLSKQPTSQPRGDPTGPKESKKKVESKTETDPCD",
    "response#3": "MEPVDPNLEPWNHPGSQPNTPCNKCYCKVCSWHCQVCFLKKGLGISYGRKKRRRRRGTPQSSKDHQNPVPKQPLPTTSGNPTGPEESKKKVASKAETDPCA"
  },
  {
    "reference": "MFEEPEWTEEAPVATDLRPVASRPLPASASQIKGSKHRQLLATLRALEAASLPQQYPSLPDSDSEEDVVERKKKRLKKASSAGASVDVEKKRKKKCQKQGPPGSDYEEKEVKGKKKCLRGILNASDPAEEQKRKRKCQKQAPSNPAQHVDKDNKTGSKAWKSTATNNQSKPSPGSTSANPPQTLSRKQWRNRQKNKRRHKNKFRPPDQAPATVPTEETEMPPAPSPDNHEDRAGALRARMAQRLDGARFRYLNEQLYSGPSSAAQRLFQEDPEAFLLYHRGFQSQVKKWPLQPVDRIAKDLRQRPASLVVADFGCGDCRLASSIRNPVHCFDLASLDPRVTVCDMAQVPLEDESVDVAVFCLSLMGTNIRDFLEEANRVLKQGGLLKVAEVSSRFEDVRTFLGAVTKLGFKVISKDLTNSHFFLFDFEKTGPPRVGPKAQLTGLKLQPCLYKRR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have methylated histone binding, methyltransferase activity within eNoSc complex, plasma membrane, nucleoplasm to regulate cellular response to glucose starvation, regulation of transcription by glucose. The designed protein sequence is ",
    "response#1": "FSRVGHIAHLNLRDHQLPYKHLIGQVIIDKNNGITCAVNKINTIESAYRNFQMEVLAGDESNMTTKVKENYLSYEFDFSKVYWNPRLSTEHARIVNLLKSDDVLFDVFAGVGPFAIPAAKKNCIVFANDLNPESYKWLQRNCKLNKVDTKVQVFNMDGREFMKGPMKEELIKQLSLKERKSSLHIVMNLPAMAIDFLDVFQDLLDGEPSSTELPIVHCHCFSKHENPAQDVQQRAEACLGTSLEGLCSVELVRNVAPNKDMMCISFCLPS",
    "response#2": "FSRVGHIAHLNLRDHQLPYKHLIGQVIIDKNNGITCAVNKINTIESAYRNFQMEVLAGDESNMTTKVKENYLSYEFDFSKVYWNPRLSTEHARIVNLLKSDDVLFDVFAGVGPFAIPAAKKNCIVFANDLNPESYKWLQRNCKLNKVDTKVQVFNMDGREFMKGPMKEELIKQLSLKERKSSLHIVMNLPAMAIDFLDVFQDLLDGEPSSTELPIVHCHCFSKHENPAQDVQQRAEACLGTSLEGLCSVELVRNVAPNKDMMCISFCLPE",
    "response#3": "LDAGCGNGRSFLVPCMVGMDYCLGLLNDARAAGGQGLVRGDVLELPFVDCSFDLVLSVAVIHHLSTRCRRERAMKEMRRVLKDGGKML"
  },
  {
    "reference": "MAEVVQVHKDWVGSFPSGKKITVVFVIGGPGSGKGTQCAKIVSQFGFTHLSAGDLLREEVKSDTEQGATIKNLMHEGKLVPSEIIVRLLLKAMLASGNDKFLIDGFPRNEENREAYEKIIKIEPEFALLIDCSREEMERRILHRNQGRDDDNMETIRRRFEVFQQSTLPVIQHYEKMGKLRRVDGDRQPDMVFEDVKAIFAQLNTQANQGSNVSRAQTNPFKSWFLDIFCSCFDVADRRN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism..2. A protein with CMP kinase activity, cytidylate kinase activity is needed to enhance phosphorylation in a specific context.3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.4. The a ribonucleoside 5'-phosphate, CMP binding site should be located in a region of the protein that is accessible to the ligand.5. The protein should have a fast turnover rate for ATP + dCMP = ADP + dCDP. The designed protein sequence is ",
    "response#1": "VIAVSGQPGSGKTTIAREIARVLGLPLVSSGLLFREMAARMGMDFIEFHKYAETNPDIDKKVDSLAIERAKAGDVVLEGHLTAWIVRPYADVCIYLKASLETRARRVALRDGKSLQDALREVAEREELNRRRYLSIYGIDINDLSIFDLVLDTD",
    "response#2": "VIAVSGQPGSGKTTIAREIARVLGLPLVSSGLLFREMAARMGMDFIEFHKYAETNPDIDKKVDSLAIERAKAGDVVLEGHLTAWIVRPYADVCIYLKASLETRARRVALRDGKSLQDALREVAEREELNRRRYLSIYGIDINDLSIFDLVLDTD",
    "response#3": "VIAVSGQPGSGKTTIAREIARVLGLPLVSSGLLFREMAARMGMDFIEFHKYAETNPDIDKKVDSLAIERAKAGDVVLEGHLTAWIVRPYADVCIYLKASLETRARRVALRDGKSLQDALREVAEREELNRRRYLSIYGIDINDLSIFDLVLDTRL"
  },
  {
    "reference": "MKKLNDLTKHLLVKELTNTGDPVITSLEMDSREVEKGSLFFCIKGYTVDGHDFARQAEERGAAALVAERPVNVNIPVVLVKDVKKAMAVMSNVFYDFPTNSFQLVGITGTNGKTTTSHLVEAMFRRASKKTGLVGTMYTKIGDRETEAKNTTPESLVLQKLFMEMREKQVEACAMEVSSHALDLGRTHGCDFNTAVFTNLTKDHLDYHHTMEAYKQAKSLLFSQLGNTYDGNRKTAVLNADDSASKDFMRMTAAQVLTYGIETECDIRAKNVVITPTETTFTLTTPWEERLVTMSLIGKFSVYNALAAAAACLAEGVSIDVIVETLEETKGVAGRFESVDAGQDFAVIVDYAHTPDSLENVLETIQGITEGRVITVVGCGGDRDATKRPEMANIAIQYSNHSIFTSDNPRTEDPQKILGDMTTGLQEKGSYQVIVDRREAIRKAVKEAGDGDVVLIAGKGHETYQDVGDKVLDFDDREEARQAIQMLKEL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that is capable of catalyzing reactions within the Cell wall biogenesis pathway with high efficiency and specificity.2. We need a protein that can perform ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate with high efficiency.3. A protein with magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, ATP binding is needed to enhance cell wall organization in a specific context.4. The protein contains novel Mur ligase N-terminal catalytic, Mur ligase central domains that confer a unique function or activity.5. The presence of Mg(2+) is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "MSLKELLHNVKIDKIIGDTNVAVAGLTSDSRKVKQGFLFIAIAGNKADGGAYALKAVQAGAVAVAAEHKLPSDTIPYVIVPDTRKAFALMSSRFYGNPSKELKLVGITGTNGKTTTSWMLEQIFKAAGKKAGITGTNGKTTVSHLVEQIVRENGEQTGLIGTMYRKIGDQILETKNTTPDSLTLQETFRDMLLSGVSTAVMEVSSHALVQGRVYGSDYDVAVFMNLSQDHLDYHHTMEEYANAKSLLFAQLGNSYHTSNPKIAVLNADDEESVRMQKATAAHVITFGIKQQADFKANNIRITSHGSTFDLRTPIGNFTLKIKMIGNFSVYNVLAAIATGATLRASDVRPGDLFAALPGARVHGADFAADAIAAGAAAVLTDLAGAARAALVADTVPVLVHPHPRHVLGDLAARVLAEENIPITGTNGKTTTATLLYELFKMRGYKVGLLSTVKNYVNDEIIEATHTTPDALALNELLKKMVQAGCQYAFMEVSSHAVEQRRIAGLKFDGAVFTNLTRDHIDYHVTFDNYLKAKKRFFDELDKNAFALTNIDDKNGTVMLQNSAARKHTYAIRRPADFKTKILEENFQGMLLNMNGRELYVPFIGKFNAYNLTAVFGSALALGLKPETIAAGLASFKGVTGRMQQVQAGKGDVHAIVDYAHTPDALAKVLETLRTVRSAGPATGANGEARILCVVGCGGDRDRTKRPVMARTAAELADLVILTSDNPRSEDPSAILGEMREGLDPIQMRKVTYNTDRAEAIRSACMQAQAGDVILVAGK",
    "response#2": "MKLTDILKGIVVISTQGDSDVEVAGLTSDSRKVKQGFLFIAIAGNKADGGAYALKAVQAGAVAVAAEHKYECPTTIVHTADSRQALAEISSAWYGTPADKLTTVGITGTNGKTTVSHLVEQIVRENGYKVGLIGTIETIIGNETIKASNTTPESLTLYNMMVKMKEAGCTYAVMEVSSHALVQHRVAGLSFKTAVFTNLTQDHLDYHKTMDAYYQAKKQLFTDYLGDNDKAVVNVDDPYGRRLAEQLPAAKITTYSMEQDADIYATNIKMTAKGTSFILNTPVGKVDINLKLVGKFNVYNALGAIGAALIEGIPLETIMEGLANLTGVPGRFQYFISDKEKVTAIVDYAHTPDALKNVLDTINTIRTKNETLITVVGCGGNRDATKRPKMAHIASANSDKSIFTSDNPRNEDPDAIIRDMEKGVESQHVSKVISIADRKQAIQTACVLANKNDIILIAGKGHENYQILGTEKIHFDDREVAAQAIAEKKA",
    "response#3": "MKEISHIAQDSRKVKPGTLFICIDGELVDGHQFASRAVELGAVAIIAEKQLDVSIPVIYVRDSKRAMAMLALHVAGLQQDSRKVKQGDCFIAVTGLETDGRRYIASALERGASVVLQEADQQQHGDVAFLSSRPIISVSNLLQKMSRIAANFYGHPSKNLTMVGITGTNGKTTVSHLVEQIVRENKKCGLMGTIKYKIGDTVIEAKNTTPDAIELQRALKEMRDAGVEYCVMEVSSHALALDRVFGIDFKAGVFTNLTRDHLDYHKTLENYLKAKKKLFKMYPKAKRVVNVDDPYGKYILENLPEDLITYGIKKEDKVYAKQIKYSVKGTEFLLSTPYGEIKVKSKLIGKFNVYNILAAVASGIVLGIPLEVIKKGLEGLENVSGRFQYFISDKEKVTAIVDYAHTPDALKNVLDTINTIRTKNETLITVVGCGGNRDATKRPKMAHIASANSDKSIFTSDNPRNEDPDAIIRDMEKGVESQHVSKVISIADRKQAIQTACVLANKNDIILIAGKGHEKYQEIKGVKYPFDDSEIVKEVLES"
  },
  {
    "reference": "MAAPASTPLNLFGLDRESLRACFAEMGEKPFRADQVMQWIYRRTADDFDLMTNISKDLRAKMKERFVIRHPELIAEQVSQDGTRKWVLKLDGGNAIETVFIPEDDRGTLCISSQVGCAMDCSFCSTAQQGLNRNMTTAEIVAQVWFAARTLGGDFQNERVISNVVFMGMGEPLANTAAVLPAIHIMLDDFGFGLSKRRVTVSTSGLVPYMDRLREDCDTALAVSLHAPNDELRDQLVPINRKYPIAELMDACRRYVAGKDRKTHIVFEYVMLDGVNDHPEHARQLAKLLAGMPAKVNLIPFNPFPQGAYRRSKPETIRQFSEILRAKRIVTTTRKTRGDDIDAACGQLVGKVQSRQKNRLSGIPVVLQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a binding site that is specific to the target S-adenosyl-L-methionine, [4Fe-4S] cluster ligand.2. The protein must exhibit the following characteristics: m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.3. The protein design must be able to perform tRNA (adenine-C2-)-methyltransferase activity, rRNA binding, rRNA (adenine-C2-)-methyltransferase activity, tRNA binding in order to contribute to rRNA base methylation.4. The protein must be engineered to have a cooperative binding mechanism with [4Fe-4S] cluster to ensure proper regulation.5. A key design requirement for the protein is the optimization of the S-methylcysteine intermediate to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "VNLMDYDAEGLAELMISYGEKPFRAKQLMKWIHQRGETDFGNMTDIAKGFREKLQAGAIVKAPGMLAEHVASDGTTKWLLDVNVGNGIETVFIPEDDRGTLCISSQVGCALECSFCSTGRQGFNRNLSVGEIIGQLWWANNRLSAEQARVGAPVNDDGRIVTNVVMMGMGEPLANFDNVVSALQMMLDDNMYGLSRRRVTLSTSGMVPAMDRLRDACPVALAVSLHASNDRIRDEIVPLNKKYPLAQLLAACNRYLEKAPRDFITFEYVMLEGVNDQPEHARELVALLRDTPCKLNLIPFNPFPNSGYNRSGRDAILAFRDILVNAGYICTVRKTRGDDIDAACGQLAGQVKDKTRRTRVVSERKP",
    "response#2": "EEGHTTQCLSSQVGCPMACTFCSTGQMGLTRNMTMGEILGQVLVAREYIAAHEDDVAPLRNIVFMGMGDPLLNVTEMVRALTTMTHDKGLGFSSRRITVSSVGILKGLEVLGDCGLAQLAISLHAPTQELREQLMPKAARQLPLDQLMAVLDRYPLKPRERVTYEYILLRGVNDRPEHARQLVKLLGGRKAKVNLIAYNPSPGAPYKAPHPDDILAFEKILWAKGLTAILRKSKGQARRAKET",
    "response#3": "EGKRRTICVSSQVGCALECAFCATARQGFNRNLTVSEIIGQVWHAARELAQPPTNVVMMGMGEPLANFDAVVKAMDIMQDDLAYMLAKQRVTLSTSGIVPNIYRLRAVSDVSLAVSLHAPNDALRDQLVPINQKYPLAELIPACKAYIGHDKRRKITWEYVMLDGVNDSLIHAKQLIRLLEGVPSKLNLIPFNPFTGSDFCTSPMERIEAFRLRLTGAGIFAMTRKTRGDEIEAACGQLAGREEGRGTLCISSQVGCTLTCSFCHTGTQKLVRNLTAAEIVGQIMMARDDLDEWPVPGAPKDETRLLSNIVLMGMGEPLYNFENVRDAMKIAMDPEGISLSRRRITLSTSGVVPEIARTAEEIGCLLAVSFHATTDEVRDKLVPINKRWNIEALLEALRAYPRLTNSERITFEYVMLNGVNDSDEDAHRLVELIEGIPAKVNLIPFNEWPGSPYTRSSNNRIHAFANIIYQAGYASPIRTPRGEDIKAACGQLRAKEEAAA"
  },
  {
    "reference": "MGGKWSKRSREGGWFAVRQKMEKTEPAAAGVGAASRDLEKHGAFTNSNTTANNADRAWVEAQEDGEEVGFPVRPQVPLRPMTYKGAIDLSHFLREKGGLEGLVWSQQRQDILDLWVYNTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPIEPKQEEETNGGETNCLLHPVSQHGMEDAEKEVLVWKFDSRLAFHHVAREKHPEYYKDC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The ideal protein would be one that plays a critical role in suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I through GTP binding within host cell Golgi membrane, membrane.2. Incorporating a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Diacidic; necessary for CD4 internalization, Dileucine internalization motif; necessary for CD4 internalization motif is critical for the successful design of the protein.3. For general function, the protein need meet that Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1 The designed protein sequence is ",
    "response#1": "MGGKWSKCSIVGWPEVRERMRRAEPAAEGVGAASRDLDKYGALTNSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGAVDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPEKVEEATEGENNCLLHPISQHGMDDPEKEVLMWKFDSRLAFHHMAREKHPEFYKNC",
    "response#2": "MGGKWSKRSAVGWPAIRERMRRTEPAAEGVGAASRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQKRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEQEKVEEANEGENNCLLHPMSQHGMEDADREVLVWKFDSRLAFHHMAREKHPEYYKDC",
    "response#3": "MGGKWSKRXGWSAIRERMRRTEPAAEGVGAASRDLERYGAITSSNTATTNEDCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQQRQDILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPLEPEKVEKANEGENNCLLHPMSQHGMEDTEREVLMWKFDSRLAFHHMARELHPEYYRNC"
  },
  {
    "reference": "MFSRKKRELMKTPSLSKKSRAGSPAPQNDLTRKDVTDSSNDLASSPPSNSSPVSSGTLKRPSSLSRHASAAGIPLSSPRGKATKPASTPSPPESGEGPFIDVEDISQLLGDVARFAERLEKLRDVVQDEELKETRRPLAHECLGEALRLLRQVINKYPLLNTVETLTAAGTLISKVKGFHYESSIENDKRDFEKALESMAVCFSSTISEFLMGEVDSSTLLSLPPGDQSQSMESLCGGLSGGEGALPSAHEYVEAGGHLGEDVDVILQRSDGGVQAALLYAKNMAKYLKDLSSYIEKRTILEMEYAKGLQKLVNAYKGTLNQETHMPFQSIYSVALEQDLEHGHGILHTALTLQHQTFLQPINMRRQEHEKRRKEVKEQWQRAQRKLMEAESNLRKARQAYMQRSEEHERALYNATRAEEEQSHSGTRSLDKKRRAEEEAKNRAEEAMATYRTCIADAKTQKQELEDVKVNVLRQLQELIKQSDQILRSATISYYQSMHMQTAPLPVGFQMLCESSKLYDLGQQYASYVRQLGAVNEPETSYDFQPYTPQITWSPCIRARKSSFNSQDIPSSENKEISGEERGVERRGGRGHQVHKSWPTAITEGDPAVSSATVPAFPEKLHQPLSPTENVDQKRLSASFEQSINGLSGSLEVQNSTGPFRNIGLSRAALTHRLRKLRTPSKCRECNSYVYFQGAECEECSLACHKKCLETLAIQCGHKKLQGRLLLFGRDFSETALRSPDHIPFLIRKCVSEIEERALIMKGIYRVN",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain that would be able to negatively regulate the actin cytoskeleton as well as cell spreading2. A protein that can perform GTPase activator activity in cytoplasm, ruffle membrane to support signal transduction.3. The protein contains novel F-BAR, Rho-GAP domains that confer a unique function or activity.4. The protein design should emphasize Basic and acidic residues, Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "SLNPIAKQIPHVVEACCSFIEKHGLSTVGIFTLEYSEKRVRKLREEFDQGLDVVLDDSQNVHDVAALLKEFFRDMKDSLLPDDLYMSFLQTATLKPQDQLSALQLLVYLMPPCHSDTLERLLKVLHKVAENCEDSIGIDGQLVSGNRMTSTNLALVFGSALLKKGASAKRESRKTRLGIDHYVASVSVVRAMIDNWDVLFQVPPHL",
    "response#2": "MSLNPIAKQIPHVVEACCSFIEKHGLSTVGIFTLEYSEKRVRKLREEFDQGLDVVLDDSQNVHDVAALLKEFFRDMKDSLLPDDLYMSFLQTATLKPQDQLSALQLLVYLMPPCHSDTLERLLKVLHKVAENCEDSIGIDGQLVSGNRMTSTNLALVFGSALLKKGASAKRESRKTRLGIDHYVASVSVVRAMIDNWDVLN",
    "response#3": "CDLQEHLQHSGQEVPQVLKSCAEFVEEYGVVDGIYRLSGVSSNIQKLRQEFESERKPDLRRDVYLQDIHCVSSLCKAYFRELPDPLLTYRLYDKFAEAVGVQLEPERLVKILEVLRELPVPNYRTLEFLMRHLVHMASFSAQTNMHARNLAIVWAPNLLRSKDIEASGFNGTAAFMEVRVQSIVVEFILTHVDQLK"
  },
  {
    "reference": "MSSLHKFKAYFGMVPLDDYEDEYLDEPEPTRRPARPARDSGRDPYHDRDDRDFAEPAFSKAAYAPGRRDDLDDDFDRYESPRHSSRVEPVAVRSARPSASGAVRGSTRGALAVDTRSDRVESRRGPLFDEGGPLSKITTLRPRDYGEARTIGERFRDGTPVIMDLVEMSNADAKRLVDFAAGLAFALRGSFDKVATKVFLLSPADIDVSAEERRRIAETGFYSQK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be optimized for stability and expression in cytoplasm where it will participate in FtsZ-dependent cytokinesis, division septum assembly.2. For general function, the protein need meet that Cell division protein that is part of the divisome complex and is recruited early to the Z-ring; Its function overlaps with FtsA.; Probably stimulates Z-ring formation, perhaps through the cross-linking of FtsZ protofilaments The designed protein sequence is ",
    "response#1": "MFKL",
    "response#2": "GFSE",
    "response#3": "LTGLIR"
  },
  {
    "reference": "MENKMADKPVARPTFFEIFKARCSESDLGPISLNWFEELSTEALPYESETSERPEYRAVCLEQSASKTPRGKPFTCSQLASTPVIFKEQSITLPLLSSPIKELGQKKTETGKDSLVNKDHRSSPHFMKTKLDQISPLNTSLSISSAALRGIYRTPQRNNIPVARGSLFCTPKLLEVKTPTCISEGLGAEVDPDMSWSSSLATPPTLSATVIIARGNDSISGAKQHDERIAMVLHSFFSSHGKRPEKNDVNVLSIPEIVNINAGLDGKDHELEKILDGSFGEMNSLENRSGRNQITQNSLEDGERNEMRIDMPDAGEHVLSVCHTSGEHLQDVTMTNWTKKTHFDKMATILLQETNEIRGNFKDASSVCERELDSPRCEIFTTNMSNNRIPKLLENRKFTKEDMPSTFTSEWSQLNLSGLDVTQLEKNSTSDKYSPSSFCNKKRSENKLHANLSTFETSLLNTSSLIKAEKLLNINWSEHMEDETKNYEKHSTSETSIMHSVDLSVQDPSLVKVCAAEKVSEVHFLDCSSFLMENTNFTGYTEVCTSCFHKELKELSKSSITDVTSHPVLCNDSIPSNPDGTKWSTNNESTPVRSCLKNTSLLSSLKKRSKRFIYTINNASPRQEEKVKKEIKAGPSIHSASSRLEFESCPFKSPDMVSNDDQDLLVSSAERKSLKSNSEESFMLLSKYATRADRIDNSPNSSLKNKMIHLDILKSNRREHQPASSLDCSETSNTLKECLVVSSNSNNVLSVEHKVIATACLRATKHSE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be involved in DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator, cellular response to ionizing radiation, spermatogenesis, intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator, centrosome duplication by localizing to nucleoplasm, centrosome, secretory granule and exhibiting identical protein binding, single-stranded DNA binding, protein C-terminus binding.2. The protein contains novel Tower domains that confer a unique function or activity.3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "FDVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCPIQIKVSTPPPPGTVIRAMPVYKKAEHVTEVVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLSQYVDDPVTGRQSVMVPYEPPQVGTEFTTILYNFMCNSSCVGGMNRRPILIIITLETRDGQVLGRRSFEGRICACPGRDRKADC",
    "response#2": "PDRSRAARDRANQKLVEYIVKARGAESHLRAILRNRKPSRWLKTFLSSGQYMTCVETYLEDEEQLDLVVKYLQGVYKQAGCQLLARGHGDGIRFILDVLLPEAIICAISAVDAVDYKTAEEKYIRGPALSSREKEIFDNT",
    "response#3": "VVGRMPECSVALPFSSISKQHAVIEILAWNKAPVLRDCGSLNGTQILRPPKVLGPGVSHRLRNRELILV"
  },
  {
    "reference": "MTDTAYRAPDLVASDIDSYLDQHAHKSLLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGQDIDFALLVDGLAAEREQGITIDVAYRFFSTDRRKFIVADTPGHEQYTRNMVTGASTADLAVILVDARKGILTQTRRHSYLAHLIGIRKLVLAVNKMDLIGYDRVKYEDIVGEYREFASTIGIDDFVPIPISGFKGDNIASRSDHTPWFTGPTLMEHLETVPLDVTADQARPFRMPVQWVARPDLDFRGFSGMIAAGRIAPGDPVRILPSGRTTTVARIVTHGGDLPLAVAGQSAMLTLADEVDCSRGDVIVRADVPAEVADQFEATILWMADEEMLPGRGYWLKLATQTVSAQLQAPKYQVNVNTMAHLPARTLGLNAIGVANLSTDRPVVFEPYAAPDGTISRDLGGFILVDKITNATVAAGLIHFALRRAANVHWQAIDVTREAHAAQKNQRARLLWFTGLSGAGKSTIANLVEKRLLALGHHSFLLDGDNVRHGLNRDLGFTDADRVENIRRVGEVARLMTDAGLIVLTAFISPFRAERDMVRAMLPPGEFMEVHIDTPLDVAESRDVKGLYAKARAGQLANFTGIDSPYEAPLNPEIRVNTIDMTPEQAAEAIVDHLLIMPA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The Phosphoserine intermediate active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.2. The protein must exhibit the following characteristics: With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway.3. Ideal protein should have a binding affinity for key enzymes in the hydrogen sulfide biosynthesis, Sulfur metabolism pathway to enhance the overall efficiency of the pathway.4. The designed protein must possess sulfate adenylyltransferase (ATP) activity to support sulfate assimilation under certain conditions. The designed protein sequence is ",
    "response#1": "TMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALENDSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADCPGHEQYTRNMVTGASTADLAVILIDARKGVLVQTRRHSYLCHLLGIRNIVLAVNKMDLIDYDQARYDAIVAEYETFAKGIGIESFTAMPISGFKGDNIKSLLRFLTCGSVDDGKSTLIGRLLYDTKLIFEDQLAALERDSRRHGTVGEEIDFALLVDGLEAEREQGITIDVAYRFFATSKRKFIVADTPGHEQYTRNMATGASTADVAVVLIDARKGVLQQTRRHSFIASLVGIRQVVLAVNKIDLEGYSQDVFDRIVADYQAFASTLGFDAIRAIPMSARFGDNVTTKSGHMPWYDGPTLLEHLETVELDDGLRYPVQYVSRPDRDFRGFCAKVASGIIKKGDTVMVLPSRKTSTVTSIVTYDGELEQAFPPQSVTVTLAHEIDISRGEMLVRPAKVDRHFEAMLVWMDESPMKNGTQFLIKHTSQTTKARIDKIQHLVDVNTLEKRNADKFELNEIGRVVITTIKPLFFDAYKKNRQTGSFIFIDPVTHNTCAVGMIRRG",
    "response#2": "KELLRFLTCGSVDDGKSTLIGRLFYDAKLIYEDTLKSLEQDSATVGTTGGELDPALFTDGLKAEREQGITIDVAYRYFSTTKRKFIIADTPGHEQYTRNMATGASTCDLAIILVDARNGVVTQTKRHSFIVSLLGIKHVLVTINKMDLVDYDEEVFDRIRSDYRDFASRLEMPDLHFMPVSALKGDNIVDASSNTPWYQGPTLMSFLDNVYIGSDRHTLRFPVQYVVRPSLDYRGFASQVASGIISKGDEVMVLPSRKKSFVKEIVTYDGNLDYAFPPQSVTITLNDEIDISRGDMLVHAAEVANQFSAKLIWMEEERLFPGRSYLLKLATRTVPATVTGLKHKIDVNTLDELAAKTLALNEIAEVNIETGAAIAFDPYSDNRETGAFILIDRETNATAAAGMIVARLLWMAPDEMLPGRTYLIKVGATTTEVTVTSLKHRININTLEHTAAKTLALNEIGVCNLSLSKAIPFAPYAESRELGSFILIDRVSNQTVGMGMLHFSLRRAQNVHWQAIDVDKHARARIKGQHPRVLWFTGLSGSGKSTIANLVEKRLHALGKHSFLLDGDNVRHGLNKDLGFTEADRVENIRRVGEVAKLMTDAGLIVLTAFISPFRAEREMVRNMLPEGEFVEIFVDTPLEVAESRDVKGLYKKARAGTLKNFTGIDSPYEAPEAPEIRVDTTKQSAEEAADMIIAQLMERGVI",
    "response#3": "KELLRFLTCGSVDDGKSTLIGRLFYDAKLIYEDTLKSLEQDSATVGTTGGELDPALFTDGLKAEREQGITIDVAYRYFSTTKRKFIIADTPGHEQYTRNMATGASTCDLAIILVDARNGVVTQTKRHSFIVSLLGIKHVLVTINKMDLVDYDEEVFDRIRSDYRDFASRLEMPDLHFMPVSALKGDNIVDASSNTPWYQGPTLMSFLDNVYIGSDRKLVDFRFPVQYVNRPNLDFRGFAGTVASGSISQGDEIVVAKSGKASRVRRIVAHGGDLRTAVAGQAITLVLEDEVEVSRGNVLAAEVANQFSAKLIWMEEERLFPGRSYLLKLATRTVPATVTGLKHKIDVNTLDELAAKTLALNEIAEVNIETGAAIAFDPYSDNRETGAFILIDRETNATAAAGMIVARLLWMAPDEMLPGRTYLIKVGATTTEVTVTSLKHRININTLEHTAAKTLALNEIGVCNLSLSKAIPFAPYAESRELGSFILIDRVSNQTVGMGGTLDFALRRAENVHWQSLDVDKRARAELKGQKPAVLWFTGLSGSGKSTIANIVEKRLHAMGRHSFLLDGDNIRHGLNGDLGFTPEDRVENIRRVGEVAKLMVDAGLIVLTAFISPFRADRDMVRAMMSPGEFLEIFVDTPLEVAESRDVKGLYKKARAGQLPNFTGIDSPYEAPEAPEIRVNTVEMTAEEAADLIVQKLLPL"
  },
  {
    "reference": "MIKQIFGKLPRKPSKSLQNDSNGEGGVNNSYYASNSSTTSISKPSSTSSKSSSASGSRVANGTLAPNSMSSNRNTNQGKKPLGGDAVVQAGPFPSSGGVYEALPSFRDVPISEKPNLFIGKLSMCCVVFDFSDPSKNLKEKEIKRQTLLELVDYVASVGFKFNDVSMQELTKMVAVNLFRTFPSANHESKILEIHDMDDEEPSLEPAWPHVQVVYEILLRFVASPMTDAKLAKRYIDHSFVLKLLDLFDSEDQREREYLKTILHRVYGKFMVHRPYIRKAINNIFYRFISETEKHNGIAELLEILGSIINGFALPLKEEHKLFLLRALIPLHKPKCSSVYHQQLSYCIVQFVEKDFKLADTVIRGLLKYWPVTNSSKEVMFLGELEEVLEATQAAEFQRCMVPLSRQIARCLNSSHFQVAERALFLWNNDHIRNLITQNHKVIMPIVFPALERNTRGHWNQAVQSLTINVRKVLCEIDQVLFDECLAKFQVEEVNKTEVKAKRERTWQRLEDLATSKTVVTNEAVLVPRFVSSVNLTTSSSESTGS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have protein phosphatase regulator activity within mitochondrion, protein phosphatase type 2A complex, cytosol to regulate negative regulation of innate immune response, brassinosteroid mediated signaling pathway, negative regulation of brassinosteroid mediated signaling pathway, response to photooxidative stress, defense response.2. Target a Polar residues compositional bias in the protein's composition for improved properties.3. The protein must exhibit the following characteristics: The holoenzyme composed of PP2AA1, PP2A4 and B'ZETA acts as negative regulator of plant innate immunity by controlling BAK1 phosphorylation state and activation in surface-localized immune receptor complexes; Involved in the regulation of reactive oxygen species (ROS) signaling.; Involved in growth regulation and stress signaling; Required for the formation of the PP2A holoenzyme that negatively regulates brassinosteroid signaling by dephosphorylating and inactivating BRI1 in the cytoplasm; The B regulatory subunit may modulate substrate selectivity and catalytic activity, and may also direct the localization of the catalytic enzyme to a particular subcellular compartment . The designed protein sequence is ",
    "response#1": "DMEDAVSIHPSFLQRNSENHHFYGVFDGHGCSHVAEKCRERLHDIVKKEVEVMASDEWTETMVKSFQKMDKEVSQRECNLVVNGATRSMKNSCRCELQSPQCDAVGSTAVVSVVTPEKIIVSNCGDSRAVLCRNGVAIPLSVDHKPDRPDELIRIQQAGGRVIYWDGARVLGVLAMSRAIGDNYLKPYVIPDPEVTVTDRTDEDECLILASDGLWDVVPNETACGVARMCLRGAGAGDDSDAAHNACSDAALLLTKLALARQSSDNVSVVVVDVTEAESFFSDVPKIGTTSVCGRRRDMEDAVSIHPSFLQRNSENHHFYGVFDGHGCSHVAEKCRERLHDIVKKEVEVMASDEWTETMVKSFQKMDKEVSQRECNLVVNGATRSMKNSCRCELQSPQCDAVGSTAVVSVVTPEKIIVSNCGDSRAVLCRNGVAIPLSVDHKPDRPDELIRIQQAGGRVIYWDGARVLGVLAMSRAIGDNYLKPYVIPDPEVTVTDRTDEDECLILASDGLWDVVPNETACGVARMCLRGAGAGDDSDAAHNACSDAALLLTKLALARQSSDNVSVVVVTEAESFFSDVPKIGTTSVCGRRRDMEDAVSIHPSFLQRNSENHHFYGVFDGHGCSHVAEKCRERLHDIVKKEVEVMASDEWTETMVKSFQKMDKEVSQRECNLVVNGATRSMKNSCRCELQSPQCDAVGSTAVVSVVTPEKIIVSNCGDSRAVLCRNGVAIPLSVDHKPDRPDELIRIQQAGGRVIYWDGARVLGVLAMSRAIGDNYLKPYVIPDPEVTVTDRTDEDECLILASDGLWDVVPNETACGVARMCLRGAGAGDDSDAAHNACSDAALLLTKLALARQSSDNVSVVV",
    "response#2": "VTEAESFFSDVPKIGTTSVCGRRRDMEDAVSIHPSFLQRNSENHHFYGVFDGHGCSHVAEKCRERLHDIVKKEVEVMASDEWTETMVKSFQKMDKEVSQRECNLVVNGATRSMKNSCRCELQSPQCDAVGSTAVVSVVTPEKIIVSNCGDSRAVLCRNGVAIPLSVDHKPDRPDELIRIQQAGGRVIYWDGARVLGVLAMSRAIGDNYLKPYVIPDPEVTVTDRTDEDECLILASDGLWDVVPNETACGVARMCLRGAGAGDDSDAAHNACSDAALLLTKLALARQSSDNVSVVVVTEAESFFSDVPKIGTTSVCGRRRDMEDAVSIHPSFLQRNSENHHFYGVFDGHGCSHVAEKCRERLHDIVKKEVEVMASDEWTETMVKSFQKMDKEVSQRECNLVVNGATRSMKNSCRCELQSPQCDAVGSTAVVSVVTPEKIIVSNCGDSRAVLCRNGVAIPLSVDHKPDRPDELIRIQQAGGRVIYWDGARVLGVLAMSRAIGDNYLKPYVIPDPEVTVTDRTDEDECLILASDGLWDVVPNETACGVARMCLRGAGAGDDSDAAHNACSDAALLLTKLALARQSSDNVSVVVLNVTRES",
    "response#3": "DMEDAVSIHPSFLQRNSENHHFYGVFDGHGCSHVAEKCRERLHDIVKKEVEVMASDEWTETMVKSFQKMDKEVSQRECNLVVNGATRSMKNSCRCELQSPQCDAVGSTAVVSVVTPEKIIVSNCGDSRAVLCRNGVAIPLSVDHKPDRPDELIRIQQAGGRVIYWDGARVLGVLAMSRAIGDNYLKPYVIPDPEVTVTDRTDEDECLILASDGLWDVVPNETACGVARMCLRGAGAGDDSDAAHNACSDAALLLTKLALARQSSDNVSVVVVDDMEDAVSIHPSFLQRNSENHHFYGVFDGHGCSHVAEKCRERLHDIVKKEVEVMASDEWTETMVKSFQKMDKEVSQRECNLVVNGATRSMKNSCRCELQSPQCDAVGSTAVVSVVTPEKIIVSNCGDSRAVLCRNGVAIPLSVDHKPDRPDELIRIQQAGGRVIYWDGARVLGVLAMSRAIGDNYLKPYVIPDPEVTVTDRTDEDECLILASDGLWDVVPNETACGVARMCLRGAGAGDDSDAAHNACSDAALLLTKLALARQSSDNVSVVVVDDMEDAVSIHPSFLQRNSENHHFYGVFDGHGCSHVAEKCRERLHDIVKKEVEVMASDEWTETMVKSFQKMDKEVSQRECNLVVNGATRSMKNSCRCELQSPQCDAVGSTAVVSVVTPEKIIVSNCGDSRAVLCRNGVAIPLSVDHKPDRPDELIRIQQAGGRVIYWDGARVLGVLAMSRAIGDNYLKPYVIPDPEVTVTDRTDEDECLILASDGLWDVVPNETACGVARMCLRGAGAGDDSDAAHNACSDAALLLTKLALARQSSDNVSVVVVD"
  },
  {
    "reference": "MITFKRLSSARWGALLTSIAVLFFLAITKGADAKAGADLADIQAYRRLRTTTADAYYASEDRVFSVVKALKDLAHNAKLTFSLDKQLKVNNRFEVLRVKQVKTDVFSSSEFTDWAHYVAKICKRGRLPADRAIFKTMAAHYGDDELARMLATAKRTSRDTVVYQLKEIQQKSWKESGKSADDVYAILQLDAGGQNVLNNPGLPAWLSYVKSPSTDYIEALLLKLREQYDDVTVAKMIVSSQSGVNKRISGQLEKELSTAWRKNHITEMEVFQLLKLNDAGTTLLKNPILEIWFHYVWKMKRNDPYELLVSWFKKAGIDDAGLGKMIATAKQDDRNYWIAQTLEQRLSGK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Attenuates cell death triggered by the pathogen-associated molecular pattern infestin 1 (INF1), indicating that the effector suppresses pattern-triggered immunity; Effector that promotes P.infestans virulence in Nicotiana benthamiana and potato; Targets host MAP3K VIK in order to utilize or promote its ability to negatively regulate immunity.2. The designed protein localizes to the host cell plasma membrane, membrane. The designed protein sequence is ",
    "response#1": "DHHHHHHPD",
    "response#2": "MPLLDSQGRVINTWADII",
    "response#3": "MISSVMKM"
  },
  {
    "reference": "MTPQGTSSSTDGLRVGIVGATGQVGAVMRQILAERRFPVSELRFFASARSAGSTLPWGDGEITVEDAATADPTGLDVALFSAGASTSRVQAPRFAEAGVTVVDNSSAFRRDPDVPLVVAEVNPHALADIPKGIVANPNCTTMAAMPVLRPLHDEAQLVRLVASTYQAVSGSGVAGVEELDGQVRAVVDKATELTHDGAAVAFPEPRKYVRPIAFNVLPMAGSLVDDGSFETDEEQKLRNESRKILGIPDLRVSGTCVRVPVFTGHSLSLNVEFARPLTVARATELLADAPGVQLTDVPTPLQAAGQDPSYVGRIRQDQSVDGDRGLVLFVSNDNLRKGAALNTVQIAELLLRR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate binding.2. The protein design should take into account the physiological conditions of the L-threonine from L-aspartate: step 2/5, Amino-acid biosynthesis pathway.3. The designed protein should be optimized for protein dimerization activity, NADP binding activity in order to improve diaminopimelate biosynthetic process.4. The protein should be designed to have high stability and activity towards L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH. The designed protein sequence is ",
    "response#1": "KYAVVGATGNVGREILQILSERGVAAKAVAAVASSRSTGMEVSYGEEDVLKVDALDDFDFSGVDMVLSSPGAKVSAEFAPRAAAAGAVVIDNSSHFRMDPDVPLVVPEVNPDALAGFRNRNIIANPNCSTIQMLVALKPIYDHFGIERINVATYQSTSGAGASAMDELAKQTANLLNAKPVEPEAFSRQIAFNCIPQIDVFLENDFTKEEMKMHWETQKILGDEGVMVNATAVRVPVFFGHAEAVHLETRMPVDVEQVKALLQQAEGVTLLEANEDFPTQVTSAAGKDNVFVGRVRKDYTHPHGLNLWVVADNIRKGAATNSIQIAELLV",
    "response#2": "EQLSHIKRVHVATYQAASGAGAMGMAELDRQTKEIAAGEEPSVEKFAYQLAYNLIPQIDVFTDNDYTKEEMKMYHETRKIMHSDIMVSATCVRVPVTRAHSEAIWLETEQPLELDAVREAFREAQGIVLQDEPADQVYPMPLFIAEKDPVYVGRLRRDLTSPNGLSFWCVADQIS",
    "response#3": "LKPIYDAVGITRINVATYQAVSGTGKDAIEELAGQTARLLNGQPAETKVYPKQIAFNVLPHIDVFLDNGYTKEEMKMVWETQKIFADANIQVNPTCVRVPVFFGHSEAVHIETEQKISAEQARELLENAPGIVVIDDHADGGYPTPVSDGAGTDPTYVGRIREDISHPNGLDMWIVSDNVRLKPIYDAVGITRINVATYQAVSGTGKDAIEELAGQTARLLNGQPAETKVYPKQIAFNVLPHIDVFLDNGYTKEEMKMVWETQKIFADANIQVNPTCVRVPVFFGHSEAVHIETEQKISAEQARELLENAPGIVVIDDHADGGYPTPVSDGAGTDPTYVGRIREDISHPNGLDMWIVSDNVREQLSHIKRVHVATYQAASGAGAMGMAELDRQTKEIAAGEEPSVEKFAYQLAYNLIPQIDVFTDNDYTKEEMKMYHETRKIMHSDIMVSATCVRVPVTRAHSEAIWLETEQPLELDAVREAFREAQGIVLQDEPADQVYPMPLFIAEKDPVYVGRLRRDLTSPNGLSFWCVADQY"
  },
  {
    "reference": "MTAVQEERTTEELKALAERAGRELEDATALEILRWAAETFGDRFCVTSSMEDAVVAHLASRALPGVDVVFLDTGYHFPETIGTRDAVEAVMDVNVITLTPRQTVAEQDAEYGPKLHDRDPDLCCALRKVKPLEEGLAGYRAWATGLRRDESETRANTPVVGWDEKRGKVKISPIAKWSQEDVQTYVTEHGVLTNPLLTDGYASVGCAPCTRRVLEGEDARAGRWAGRSKTECGLHG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein is the optimization of the Nucleophile; cysteine thiosulfonate intermediate to achieve high catalytic efficiency for [thioredoxin]-disulfide + AMP + 2 H(+) + sulfite =   [thioredoxin]-dithiol + adenosine 5'-phosphosulfate.2. Ideal protein should have a binding affinity for key enzymes in the Sulfur metabolism, hydrogen sulfide biosynthesis pathway to enhance the overall efficiency of the pathway.3. The designed protein should have 4 iron, 4 sulfur cluster binding, adenylyl-sulfate reductase (thioredoxin) activity, phosphoadenylyl-sulfate reductase (thioredoxin) activity to facilitate sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin).4. For general function, the protein need meet that Catalyzes the formation of sulfite from adenosine 5'- phosphosulfate (APS) using thioredoxin as an electron donor. The designed protein sequence is ",
    "response#1": "TGFGPSGIVIMHMLADLRPGTTVFYLDTDLLFPETYELCDDLDERLDVDVTRVHGGLSLDEQAEQEGEELWNRNPNRCCFLRKVKPLRNFLDDRRAWITGVRRDQSERRADTDILSWEGQYGVFKINPLANWTQKEVWKYLFEHDLPYNPKHDQGYPSLGCVPCTEYPWEGRHVLSSSFGTQSAVMLHLVSRQMPEIPVILVDTGYLFPETYRLVDALTDRFGLNLKVYRPALSPAWQEAGLGRLWEQGADGIERYNRLNKIDPMERALRDLDAGTWFAGLRRQQANSRAELPVLRRQDGRIKFHPIIDWHRPRRARYLRRHDLPDHPLRDQGYVSIGDVHTTVKGGEYDGAISFSYTTGFGPSGIVIMHMLADLRPGTTVFYLDTDLLFPETYELCDDLDERLDVDVTRVHGGLSLDEQAEQEGEELWNRNPNRCCFLRKVKPLRNFLDDRRAWITGVRRDQSERRADTDILSWEGQYGVFKINPLANWTQKEVWKYLFEHDLPYNPKHDQGYPSLGCVPCTEYPWEGR",
    "response#2": "VASNMQDGVLVHLAAQVHPGVDVLFLDTGYHFPETIGTRDAVEQVYGVNVINARALASVAEQDVAEGKDLFARDPNRCCALRKVAPLKQTLSGYSAWVTGIRRVEAPTRANAPLISFDEAFGLVKINPIAPWSDEEMQNYIDTNSILVNPLVDEGYPSIGCAPCTAVFDSASGETRGYGRCKHHLNLAA",
    "response#3": "TGFGPSGIVIMHMLADLRPGTTVFYLDTDLLFPETYELCDDLDERLDVDVTRVHGGLSLDEQAEQEGEELWNRNPNRCCFLRKVKPLRNFLDDRRAWITGVRRDQSERRADTDILSWEGQYGVFKINPLANWTQKEVWKYLFEHDLPYNPKHDQGYPSLGCVPCTELVAFEDGAQAVLSSSFGIQAAVSLHLVTSQQPNIPVILTDTGYLFPETYQFIDQLTEQLKLNLKVFRAYISPAWQEARYGKLWEQGIKGIQLYNKINKVEPMNRALIQLGSLTWFAGLRRTQSSSRSKLPVLAVQQCLFKLLPIIDWDNRQVHSYLKKHGLNYHPLWEQGYLSVGDTHTTGFGPSGIVIMHMLADLRPGTTVFYLDTDLLFPETYELCDDLDERLDVDVTRVHGGLSLDEQAEQEGEELWNRNPNRCCFLRKVKPLRNFLDDRRAWITGVRRDQSERRADTDILSWEGQYGVFKINPLANWTQKEVWKYLFEHDLPYNPKHDQGYPSLGCVPCTELVAFEDGA"
  },
  {
    "reference": "MTHYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTMLKLDPYINVDPGTMSPIQHGEVFVTDDGAETDLDLGHYERFIRTRMTKRNNFTTGRVYEEVLKRERRGDYLGATIQVIPHITNEIKRRIIDGSEGVDVAIVEIGGTVGDIESQPFIEAIRQLGVEVGRNRAMFMHLTLVPYLAVSAEVKTKPTQHSVKELRSVGVQPDILVCRSEVPLPSNERQKIALFTNVIDKAVISLRDADSIYKIPAMLKAQGLDELVVERFGLDCPEADLTEWEQVLYAESNPTGEVTIGMVGKYVELPDAYKSVNEALKHAGLKNRLTVNIRYIDSQDIESKGEHILEGLDAVLVPGGFGERGIEGKIAAAKYARENQIPYLGICLGMQVALIEYARNVAGMENANSTEFAPDTPFPVVGLITEWLDAAGTTESRDESSDLGGTMRLGSQLCHLLPDSKVHDMYGASEIYERHRHRYEVNNNLRGKVEAAGLKVTGLSADKRLVEVIEIAEHPWFVAGQFHPEFNSTPRDGHPLFTGFVKAAGEYFKNHN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein have Glutamine amidotransferase domains with specific sequence motifs that are crucial for its activity.2. The protein must exhibit glutaminase activity. The designed protein sequence is ",
    "response#1": "PDAYKSLREALVHGGIANRVKVNINWLDAELFEGADGELAGKLEPCHAILVPGGFGERGSEGKIASVTFARERKVPFFGICLGMQMACIEGARNTAGIAGASTTEFGETSEPVVGLITEWMSKEGLQERAAGGDLGGTMRLGAYEAKLSGNSHVASIYGTTDISERHRHRYEVNAHYKPALEEGGLVFSGMSPDGRLPEIVERPDHPWFIGVQFHPELKSRPFAPHPLFASFIGRV",
    "response#2": "RIAIVGKYVRLEDAYLSVSEALRHAAWQQNASVEIEWIDSETLEGEEGESVRERLADVDGILIPGGFGGRGIEGKIAAAQIAREQAIPYLGICLGMHVAVAEFARNVAGMEGANSTEMDVETPYPVIDLLPEQKEVSDLGGTMRLGADPIKLHSGSLAREIYGEAVVYERHRHRYEVNNLLRKRLEAAGLRASGTSPDERLVEVIELAEHPFFVASQFHPEFKSRPERPAPLFRDFR",
    "response#3": "LPDAYLSVTEALRAGGFAELTKVNIHWVRSDDCETEQGALSNLGEMDAICVPGGFGVRGIEGKLGALKFARENQIPTLGLCLGLQCMVIEFARNVAGITDATSSEFDPEGKNPVIATMAEQVDKIASSNLGGTMRLGLYEANLLPGSVVSKTYGSVLASERHRHRYEVNNSYREQLASAGLVFSGTSEKDSLVEYVELPADVHPYYVSTQAHPEFKSRPTKPNPLFHGLVEDMAAL"
  },
  {
    "reference": "MDLTKMGMIQLQNPSHPTGLLCKANQMRLAGTLCDVVIMVDSQEFHAHRTVLACTSKMFEILFHRNSQHYTLDFLSPKTFQQILEYAYTATLQAKAEDLDDLLYAAEILEIEYLEEQCLKILETIQASDDNDTEATMAEGGAEEEEDRKARYLKNIFISKHSSEESGYASVAAQSLPGPMVDQSPSVSTSFGLSAMSPTKAAVDSLMTIGQSLLQGALQPPAGPEEPTLAGGGRHPAVAEVKPEMMQVDEVPGQESPGTVESSISAGMGDKVEERGKEGPGTPTRSSVITSARELHYGREESTEQLPPPVEAGQGPPGRLEPPAHPAEKHLGLYSVLPNHKADAVLSMPSSVTSGLHVQPALAVSMDFSTYGGLLPQGFIQRELFSKLGELAVGMKAESRSVGEQCSVCGVELPDNEAVEQHRKLHSGMKTYGCELCGKRFLDSLRLRMHLLAHSAGAKAFVCDQCGAQFSKEEALETHRQTHTGTDMAVFCLLCGKRFQAQSALQQHMEVHAGVRSYICSECNRTFPSHTALKRHLRSHTGDHPYECEFCGSCFRDESTLKSHKRIHTGEKPYECNGCGKKFSLKHQLETHYRVHTGGLEGKLLIDIGSGPTIYQFLSACESFQEIIATDYTDKNLQELEKWLKKMPGAFDWSPVVKYVCELEGNRDKWAEKEERVRRAVTQVLKCDVLKERPLEPAVLPPADGLISSLCLEAACPTPQACRDALRHLRTLLRPGGHLVLSGGFETTFFMVGDKRFSTLPLNEKFLR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be able to regulate mesonephros development. The designed protein sequence is ",
    "response#1": "IVGGRPAPARKWPWQVSLQVHKQHICGGSLISKWWVITAAHCVYGHLDYAVFMGDADLWSKRPVRIPVQDIIVHQDFSMMRTVVHDIALVLLAFPVNYSVNIQPVCIPEKSFLVQPGTLCWVTGWGKVLEQGRSSRILQEIELNIIRHEKCNQILKDIMGNIFTLVQEGGVCGYNEKGGDACQGDSGGPLVCEFNKTWVQVGIVSWGLGCGRIGYPGVYTEVSYYRDWIQ",
    "response#2": "IVGGRPAPARKWPWQVSLQVHKQHICGGSLISKWWVITAAHCVYGHLDYAVFMGDADLWSKRPVRIPVQDIIVHQDFSMMRTVVHDIALVLLAFPVNYSVNIQPVCIPEKSFLVQPGTLCWVTGWGKVLEQGRSSRILQEIELNIIRHEKCNQILKDIMGNIFTLVQEGGVCGYNEKGGDACQGDSGGPLVCEFNKTWVQVGIVSWGLGCGRIGYPGVYTEVSYYRDWIAG",
    "response#3": "RIVGGRPAPARKWPWQVSLQVHKQHICGGSLISKWWVITAAHCVYGHLDYAVFMGDADLWSKRPVRIPVQDIIVHQDFSMMRTVVHDIALVLLAFPVNYSVNIQPVCIPEKSFLVQPGTLCWVTGWGKVLEQGRSSRILQEIELNIIRHEKCNQILKDIMGNIFTLVQEGGVCGYNEKGGDACQGDSGGPLVCEFNKTWVQVGIVSWGLGCGRIGYPGVYTEVSYYRDWIR"
  },
  {
    "reference": "MSVTTEFSPRPAPSPGFGHNSGDRPNLVGMTREELGAALVAVGVPERQVRMRVAQLWHWIYVRGADRFHAMTNVAKDLRARLDDVYTLARPEIVTEQVSRDGTRKWLLRFPSRGAGRPVEIETVYIPEEGRGTLCVSSQVGCTLTCSFCHTGTQKLVRNLTAEEILAQILVARDRLGDYPDATPNAEAATPSEGRLVSNIVMMGMGEPLYNFDNVRTALLTATDGDGLSLSKRRVTLSTSGVVPNIARTGDEIGVMLAISLHAVRDDLRDVLVPLNRKYPLKELLDACRAYPGLSNARRITFEYVMLKGVNDSDADARELVRLLRGIPAKINLIPFNPWPGSPYECSDWERIEGFAELVNAAGYASPIRTPRGRDILAACGQLKSESERLRKKEREALETLGLTETDLAMRAMVAGHGED",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a high specificity for S-adenosyl-L-methionine binding, with minimal off-target effects.2. The protein requires [4Fe-4S] cluster to activate a critical catalytic site.3. The protein must be able to tRNA (adenine-C2-)-methyltransferase activity. The designed protein sequence is ",
    "response#1": "MTACVSSQVGCSLTCKFCATGYMERKRNLDPAEIYDQVVMIDKQAREANNVPLTNIVYMGMGEPLLNYANVLKSVDYITSPDGLGMSPKRITVSTAGIAKMIKKLGDDEVKFNLALSLHAANDEKRNQIMPINESNTLEALKEALQYFYQKTKNKITFEYIMFYSFNDTLKDAEELFQFTKSVPSKVNIIEYNPIAEATYKNADPEALDKFSARL",
    "response#2": "MTACVSSQVGCSLTCKFCATGYMERKRNLDPAEIYDQVVMIDKQAREANNVPLTNIVYMGMGEPLLNYANVLKSVDYITSPDGLGMSPKRITVSTAGIAKMIKKLGDDEVKFNLALSLHAANDEKRNQIMPINESNTLEALKEALQYFYQKTKNKITFEYIMFYSFNDTLKDAEELFQFTKSVPSKVNIIEYNPIAEATYKNADPEALDKFSARL",
    "response#3": "MTACVSSQVGCSLTCKFCATGYMERKRNLDPAEIYDQVVMIDKQAREANNVPLTNIVYMGMGEPLLNYANVLKSVDYITSPDGLGMSPKRITVSTAGIAKMIKKLGDDEVKFNLALSLHAANDEKRNQIMPINESNTLEALKEALQYFYQKTKNKITFEYIMFYSFNDTLKDAEELFQFTKSVPSKVNIIEYNPIAEATYKNADPEALDKFSAL"
  },
  {
    "reference": "MDREQKSLYRVLLIVSGGIAAYKSAELVRELRGRGCDVRVAMTSGAQHFITPLTLQALSGNRVHTELLDAEAEAGMGHIELARWAEQVLVAPATADFLARLAQGRADDLPAALCLATRCPIAVAPAMNNAMWEAPATRDNIAILQRRGVTFIGPVSGDQACGESGMGRMCEAREIAAALLSSGKQAGAAPPSAPASQDDTTDGAFDYLPLAGKAVMVTAGPTREPLDPVRYLTNRSSGRMGFAIAQAAHSAGARVILIAGPCALPTPAGVERHDVERASDMYSAVMARIDEADIFIGCAAVCDYRPIDEAHEKIKKGAQGIDLALVPTPDILRDVASRSPRPFVVGFAAETHDVQSHARGKLLAKNLDMIAANQVGDGQAFDVDENSLELLWDGGGKSLPQQPKHELALALIKVIVERMGLP",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that In the first step cysteine is conjugated to 4'- phosphopantothenate to form 4-phosphopantothenoylcysteine; Catalyzes two sequential steps in the biosynthesis of coenzyme A2. A protein that is capable of catalyzing reactions within the coenzyme A biosynthesis, Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway with high efficiency and specificity.3. The protein should be able to bind CTP ligand in a variety of conditions4. A protein with metal ion binding, phosphopantothenoylcysteine decarboxylase activity is needed to enhance pantothenate catabolic process in a specific context.5. The protein must bind to Mg(2+) in order to perform its enzymatic function.6. The presence of Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.7. The protein should be designed to have high stability and activity towards H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2. The designed protein sequence is ",
    "response#1": "MRILLGVAGGIAAYKAVLLLRLLREAGHDVRVIPTHAALEFVGRPTWEALSGHPATPEVFEDVEHVPHVSLGQGADLLVVAPATADLLSRAATGRADDLLTSTLLTVRCPVLLAPAMHTEMWDHPATRANVATLRQRGVHVLEPASGRLTGSDTGPGRLPEPAEIAAAALEVLGSPADQLAGRRVLVTAGGTHEPIDPVRFLGNRSSGKMGYALAIEARNRGASVILVSGPVALPPPYGVEMQQVETAIQMRNAVHRFITDVDVLVMSAAVADFRPDEVATQKIKKHSDEVQTHDGKLSLHLVPNPDILGELADTRNGSLEDSGRRLIRVGFAAETQQVVDYAQSKLHSKGLDLLVANDVSRSDSGFGTETNKVLLASGEHTEVPEWPKEQLADALLDRVLPRLRAG",
    "response#2": "MRILLGVAGGIAAYKAVLLLRLLREAGHDVRVIPTHAALEFVGRPTWEALSGHPATPEVFEDVEHVPHVSLGQGADLLVVAPATADLLSRAATGRADDLLTSTLLTVRCPVLLAPAMHTEMWDHPATRANVATLRQRGVHVLEPASGRLTGSDTGPGRLPEPAEIAAAATTLLTDPSRRSEPGLEGLTVLVTAGGTREPLDPVRFVGNRSSGRQGVALALAAAARGARVTLLAAHLDVEAPAGVTVHHVGTALELREAALAAAPGVDIVIMAAAVADYRPIEVAEAKIKKEQQGERLVLELVKNPDILAEISAGRHEGQLVVGFAAETEPDREAMLALGRAKIARKGCDLLVLNRVGWAEGFQSGSNAVVVGADGAETAVPSGSKQLLAATVWDAVVRLRGG",
    "response#3": "MRILLGVAGGIAAYKAVLLLRLLREAGHDVRVIPTHAALEFVGRPTWEALSGHPATPEVFEDVEHVPHVSLGQGADLLVVAPATADLLSRAATGRADDLLTSTLLTVRCPVLLAPAMHTEMWDHPATRANVATLRQRGVHVLEPASGRLTGSDTGPGRLPEPAEIAAAVLGALTAPVPDLDLAGRHVVVSAGGTREPIDPVRFLGNRSSGRQGLALARAARARGARVTLVAANVTLPDPDGVDVVHVESTAELRDAVHAAAKDADVVVMAAAVADFRPASTAGHKIKKRDDGVVAPIALEANPDVLAELAGELRRPGQVVVGFAAETGDDAGDVLEHGRHKARRKGADLLAVNAVGGGLGFGTADNEVTILTADGEPVELPRASKDEVADALWDAVRRLR"
  },
  {
    "reference": "MPNRDLRRPAEARPQPLTGLAGFLDVPLTGGPSDPEATLTGVTHDSRAVRPGDLYAGLQGEHVHGAAFATQAAEAGAAALLTDPAGAALAAGSGLPALVVADPRGRLGEIAAWVYGRPAEHLTMYGVTGTNGKTTTAYLLDGGLRAGGGVTGMIGTVETRIGEEVVPSVRTTPEATDVQALLASMLERGVTDAVMEVSSHALALGRVDGTVYDVALFTNLSQDHLDFHPSMEDYFLTKADLFTPRRSRLGVIDVDDAYGRRLAKLASVPVLTVSATGDQDADWRAENVELGPDGSRFTVVGPDCHKAEARVRLAGPFNVSNALLAIVALTAGGVSLDDAVEGVGRTPGVPGRMERVDVGQPFEAVVDFAHTPDAVETLLRALRPVTSGALTIVLGAGGDRDRAKRPLMASAAARFADAVVLTSDNPRSEDPNTILDEMLAGIRAADAAPGAVLVEPDRVAAIALAVDRAAPGDTVVIAGKGHEQGQEFANGVKHPFDDRVELRAALARTMHAGPASPKQSTDARIVAPAPSTHTPTSETPNPGVPATGTSRAEETNQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should include a Meso-diaminopimelate recognition motif motif to ensure proper function.2. The protein should exhibit Polar residues compositional bias.3. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.4. Ideal protein should have a binding affinity for key enzymes in the Cell wall biogenesis, peptidoglycan biosynthesis pathway to enhance the overall efficiency of the pathway.5. The designed protein have Mur ligase central, Mur ligase N-terminal catalytic, Mur ligase C-terminal domains with specific sequence motifs that are crucial for its activity.6. The designed protein should be involved in regulation of cell shape, cell wall organization, cell cycle, peptidoglycan biosynthetic process, cell division by localizing to cytoplasm and exhibiting ATP binding, magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity.7. The designed protein should be able to compete with endogenous proteins or inhibitors for meso-2,6-diaminoheptanedioate, ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding. The designed protein sequence is ",
    "response#1": "MKLEISGLTADSRQVRPGDLFVAVAGTKANGTAYIADAVSRGAAAIVAADAHPDAGAPVFMLPDPRRFLAKAAAAFYGYPSSKLTLVGVTGTNGKTTTTHLIEKILDDHGHLAGLIGTIKMKIGNEVFDVKNTTPDALELQKSFAKMLDVGSDYAIMEVSSHALDIGRVRGVQYHIAVFTNLTQDHLDFHETMEKYREAKGLLFSQMGNTYDSNVKKNKYAVLNADDRASEYYKRITAAQVITYGIDKEADVRATKIVISSHGTSFILESFRGTIEINLKMIGKFSVYNALAATAVGRLERCDGPEDDVLVAVDYAHTPDALGRVLAAVRALGAGRVLCVFGCGGDRDASKRPLMGEAVGRGADHAYVTSDNPRSEAPADIVAAILPGLGAASARYDVELDRALAIEKAVLEASPGDVVLIAGKGHENYQEIQGVRHPFDDKQVATEALEARL",
    "response#2": "MIHINGMTLDSRKVRPGEMFLAMRGVHHDGRAFIAEAAARGAAVICVDGPVSAADREAAAGAVILGIDELGTAAGVLAATDSRRVQRGDAFIAWPGYGVDARQFVPSALKNGAVACLVEADGADAFALDGAVCAAVPDLKSVTGELAHRFYGRPTAQLRMIGVTGTNGKTTTTHLIEKILDDHGWRAGLIGTVETIIGDARLPASNTTPESFDLQGLFAAMRDAGCTHVVMEVSSHALDQERVRGVRFEIAAFTNLSQDHLDYHGTMEAYADAKARLFDHQGLAAAVLNADDPAAAAMAARTAVPVVSYGPAGSDVRATGWRATADGIEAELVTPWGRQRLRTPLIGRFNVSNILAAIGATLALGVPLDEAAGALESLEAVPGRLELVGQTTDGALAYVDYAHKPDALENVLESVRPFTTGRVIIVFGCGGDRDKGKRPIMGEIAARLADIVIVTDDNPRSEIPEVIRSEIMAGARGATEISDRAEAIRTAVGMLKAGDTLIVAGKGHETYQIIGAETLHFDDRETARAALRARR",
    "response#3": "MSLATDSRRVQRGDAFIAWPGYGVDARQFVPSALKNGAVACLVEADGADAFALDGAVCAAVPDLKSVTGELAHRVHAHPDPALRVVGVTGTNGKTTTTHLIEKILDDHGHLAGLIGTIKMKIGNEVFDVKNTTPDALELQKSFAKMLDVGSDYAIMEVSSHALDIGRVRGVQYHIAVFTNLTQDHLDFHETMEKYREAKGLLFSQMGNTYDSNVKKNKYAVLNADDRASEYYKRITAAQVITYGIDKEADVRATKIVISSHGTSFILESFRGTIEINLKMIGKFSVYNALAATAVAGLVAAGVDTATLAAGIAACPGVPGRLEEVSHLPNGASVYVDYAHTPDALETALHALRPHTKGRLIVVFGCGGDRDPGKRPQMGKIASQSADIAFVTDDNPRTENAAAIRQQVLDGGEGLREIGDRAAAIQTAVRLLQAGDCLIVAGKGHEDYQIVGTERHPFDDREVAAAALRAREEPAA"
  },
  {
    "reference": "MLKDKSIVVGVTGGIAVYKVVDVVSRLKKQNANVEVIMTTHATEFVSPLTFQTLSLNPVHVDMFKEPKNYDVEHIALAEKADIFLIAPATANIIGKIANGIADDLLTTTIMATKAKVIFAPAMNTNMYSNPIVQRNIDYLKDLEYEFIEPGTGFLACQTYGPGRMAEPIDIVEYLKSNYSNKDLKGKKVVVTAGPTIEPIDPVRYITNYSSGKMGYSIAEEAYKRGAETILITGPTSLVPPKGVKVIKVNTTMEMFNAVEEEFDSCQVLIKAAAPSDYRPETIRDKKIKKNEKNHELAIRFTRNPDIAAHFGKIKKDRIIVGFAAETNDLIQYAKQKLEKKNFDFIVANDILKEGAGFKSDTNIVTIIDKSDKVVDYPILDKYQVAKIILDRIQNLLSIKS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should be able to enhance the efficiency of coenzyme A biosynthetic process. The designed protein sequence is ",
    "response#1": "MKIVLGVSGGIAAFKSCHVVRLLKEQGHDVQVIPTATSLEFIGRATWEALSGNSVSTSVFDGVPEVRHVSIGHTAELFIVAPATADVIARLAHGRADDLLTASALMTRCPVVVFPAMHTEMWTHPATQANVATLRQRGVTVVDPAHGRLTGPDSGPGRFAEPEQVVNLASL",
    "response#2": "MHVVVGVSGGIAAYKACSLVRRFTEGGHDVTVVPTAAALRFVGAPTWAALSGKPVSTEVWTDVHEVPHVRIGQSADLVVVAPATADIMAKAAAGIADDLLTNTLLTARCPVVFAPAMHTEMWEHPATQGNVETLRRRGAHVIEPAEGRLTGADSGKGRFPEPEDVYAAALSA",
    "response#3": "MHVVVGVSGGIAAYKACSLVRRFTEGGHDVTVVPTAAALRFVGAPTWAALSGKPVSTEVWTDVHEVPHVRIGQSADLVVVAPATADIMAKAAAGIADDLLTNTLLTARCPVVFAPAMHTEMWEHPATQGNVETLRRRGAHVIEPAEGRLTGADSGKGRFPEPEDVYAAAIEGRL"
  },
  {
    "reference": "MMDSRPLIFDRSPEELTQILQEWGEPPYRVKQLWWGLYRNLWNKADQFTVFPKALRQKLEEQFAFESLQAVRESWSDNGQTTKLLFHTLQGKPLETVLMRTLDRNTLCISSQSGCGMGCVFCATGQMGFMANLTSGQMIEQVLYFVRILKEENRTLSNIVVMGMGEPFHNFENVLAAIDRLNDPNGFNFGERRFTISTVGIVPFIRKFAHLKRQINLAVSLHAATDELRSELVPINRKYPLKTLLEACHEYVNTTRRRLSFEWVMIRDVNDSLEQASQLVKLLKGLLCHVNLIPLNPTDQYSGKAPPKHRIQAFKALLEQHQIPCTVRVPRGVSIKAGCGQLAALHQGQTS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECN",
    "response#2": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECN",
    "response#3": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECV"
  },
  {
    "reference": "MSRYQSHSFDDGEINPFANPTSVPAATSKLSPLPPEPYDRGATMDIPLDSGKDLKAKEKELREKEAELKRREQEIKRKEDAIAQAGIVIEEKNWPPFFPLIHHDISNEIPIHLQRIQYVAFTSMLGLVVCLLWNIVAVTTAWIKGEGPTIWFLAIIYFISGVPGAYVMWYRPLYRAMRTDSALKFGWFFFTYLFHIAFCVFAAVAPPIIFKGKSLTGILPAIDVLSGNILVGIFYFIGFGFFCLESLVSIWVIQQVYMYFRGSGKAAEMKQEATRRAMMAAL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.2. The protein should have a specific function in protein transport and should be localized to trans-Golgi network membrane, recycling endosome membrane, plasma membrane in order to carry out this function efficiently.3. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "NTILYRDRRSLTAHVSFDGGEEWETVKGNDGTIEGTVDLIWRHPFDDNRAYILGENGKHWITTDQAKSWRPFQVDVLPFASMRSFPLEFNGWDPKKVIFMGLDCTILGCLPQTFYTTDDFETVKPLREMTLQCMWAASRPQFALDIDMPKSTGDRILCVVPGLKGPFEHTRTERLIYSDSFFKDDAEGTEAKLDHGKPLSGVSLQIRSVSKYIVARLQSRGTQEQALYVSDDSNVWHRAEFGKHRIEEDAYTILESTNYSIQIDVQTTWQDNRMGSLFSSNSNGTYFTQNIEHTNEDLNGLVDFERVTGIHGITIVNTVKNWEEAKKSDSNKKKLISSISFDDGRTFQPLKADKDELHLHSMTTYAALHEMPLPDHRMFSSPAPGLVMGVGNTGGHLEDYSKGDLYVSDNAGLTWRRALKGPHRYEFGDQGGVITAVLDSSQTDKVQFSIDHGKEWESISLGQKIRPLFLVSTPDSTSLKFLLVGTSSDDTTKTLMFFIDFDGLHERECGSGDLEEWTARLNEKGEPDCLMGQKQFFQRRKANADCFIKKEFAISGPEFQLCKCSAEDFECDYNFVRSEDRKECVPAVPLTPPAGKCKDPSDKYMGPSGWRLIPGNACIREGGEDLEREIERSCSNVTGPPR",
    "response#2": "NTILYRDRRSLTAHVSFDGGEEWETVKGNDGTIEGTVDLIWRHPFDDNRAYILGENGKHWITTDQAKSWRPFQVDVLPFASMRSFPLEFNGWDPKKVIFMGLDCTILGCLPQTFYTTDDFETVKPLREMTLQCMWAASRPQFALDIDMPKSTGDRILCVVPGLKGPFEHTRTERLIYSDSFFKDDAEGTEAKLDHGKPLSGVSLQIRSVSKYIVARLQSRGTQEQALYVSDDSNVWHRAEFGKHRIEEDAYTILESTNYSIQIDVQTTWQDNRMGSLFSSNSNGTYFTQNIEHTNEDLNGLVDFERVTGIHGITIVNTVKNWEEAKKSDSNKKKLISSISFDDGRTFQPLKADKDELHLHSMTTYAALHEMPLPDHRMFSSPAPGLVMGVGNTGGHLEDYSKGDLYVSDNAGLTWRRALKGPHRYEFGDQGGVITAVLDSSQTDKVQFSIDHGKEWESISLGQKIRPLFLVSTPDSTSLKFLLVGTSSDDTTKTLMFFIDFDGLHERECGSGDLEEWTARLNEKGEPDCLMGQKQFFQRRKANADCFIKKEFAISGPEFQLCKCSAEDFECDYNFVRSEDRKECVPAVPLTPPAGKCKDPSDKYMGPSGWRLIPGNACIREGGEDLEREIERSCSNVTGPPL",
    "response#3": "NTILYRDRRSLTAHVSFDGGEEWETVKGNDGTIEGTVDLIWRHPFDDNRAYILGENGKHWITTDQAKSWRPFQVDVLPFASMRSFPLEFNGWDPKKVIFMGLDCTILGCLPQTFYTTDDFETVKPLREMTLQCMWAASRPQFALDIDMPKSTGDRILCVVPGLKGPFEHTRTERLIYSDSFFKDDAEGTEAKLDHGKPLSGVSLQIRSVSKYIVARLQSRGTQEQALYVSDDSNVWHRAEFGKHRIEEDAYTILESTNYSIQIDVQTTWQDNRMGSLFSSNSNGTYFTQNIEHTNEDLNGLVDFERVTGIHGITIVNTVKNWEEAKKSDSNKKKLISSISFDDGRTFQPLKADKDELHLHSMTTYAALHEMPLPDHRMFSSPAPGLVMGVGNTGGHLEDYSKGDLYVSDNAGLTWRRALKGPHRYEFGDQGGVITAVLDSSQTDKVQFSIDHGKEWESISLGQKIRPLFLVSTPDSTSLKFLLVGTSSDDTTKTLMFFIDFDGLHERECGSGDLEEWTARLNEKGEPDCLMGQKQFFQRRKANADCFIKKEFAISGPEFQLCKCSAEDFECDYNFVRSEDRKECVPAVPLTPPAGKCKDPSDKYMGPSGWRLIPGNACIREGGEDLEREIERSCSNVTGPPR"
  },
  {
    "reference": "MVLDEKNKNPVKLVIAGHVDHGKSTLVGRLLHDSNLLPDGKIKSIQEMSERRGMPLEYAFLLDSLQAERDQGITIDFTQIPFKSSKRAYVIIDAPGHKEFLKNMISGAAQADAGIIVIDAFEGLQEQSRRHCYLLKLLGFNSIAVVVNKMDLIQYDQKIFKQISETLAKYLNQIGMHALNIIPVSAREGEGLTTVSKHMTWYEGLTFVEAIDSIEQKPPLTNAPLRLPIQDIYKFDDRRILAGRVETGTLSLGDKVLFSPSNKTARISSFETWGETKTSEKVAAGESVAFTLDEQIFVERGHIASDLKSPPIETNVFKARLFWLSEKPLLISQRMTLKLATSEYSVEIQSIEKCIDTQSLEPLPANNIKRNEIGDVTIRSKGTVVLDPHDVNAKMGRFTLIDQYETVGGGIISMEGYPDQRGRYDVVSKNIEVVASKVDQSTRASVNEHQSGIIWLTGLSGAGKSTIALETERQLFQKGYQVYVLDGDNIRFGLSADLGFAPEDRTENIRRIGEVAKLFADAGILVITAFISPYRDDRDRVRAIAPDLFHEVFVKADLSTCETRDPKGLYAKARRGEIEDFTGISAPYEEPNEAELVIDTGEESIDQSVARLIDYVSSNFKL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that is capable of catalyzing reactions within the Sulfur metabolism pathway with high efficiency and specificity.2. The designed protein should be optimized for adenylylsulfate kinase activity activity in order to improve phosphorylation.3. The protein must exhibit the following characteristics: ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity.. The designed protein sequence is ",
    "response#1": "LDTLRFITCGHVDDGKSTLIGRLLHDSQALFDDQLATLQSDSRRIGTQGEEIDFALLVDGLAAEREQGITIDIAWRFFTTDKRRFIIADAPGHQEYTRNMATAASTAMLAVILVDAGAGIREQTRRHAMICNLMGISQIIVAVNKIDSVGYDRTVFERIEAEFDDFASSLNFTSVTAIPISALKGDMVISRGSSLDWYHGPTLMGALELASTDDASLEQGITIDVFRFPVQYVNRPNLDFRGFAGTVASGSVSRGDEVVVAKSGRSAHVKRIVSYGGDLQKAVAGQAVTLVLDEEVEVSRGNVLAAREPCTAFTADVVWMHETPLMVGHEYEFKAATRVVSGRVVRIVYQVDVNTMARSATDTLALNGIARCQIEVTQPLVLDSYQRSPDTGNFIFIDKLNNLTVGAGMFKATLVWMDQAELLPGRAYWLKLGTKTVSAVVRAPEYAIDVNTMARGAVKTLALNDIGIAEIATDRPIVFEPYAESHDLGGFILIDKLTNATVGAGMLHFALREPCTAFTADVVWMHETPLMVGHEYEFKAATRVVSGRVVRIVYQVDVNTMARSATDTLALNGIARCQIEVTQPLVLDSYQRSPDTGNFIFIDKLNNLTVGAGMVRG",
    "response#2": "RFITCGSVDDGKSTLIGRLLHDSIGLLEDQIAGLKDDSRRFGTTGEELDLALLLDGLEAEREQGITIDVAYRFFSTARRAFVVADTPGHAQYTRNMATGASTAELAVLLVDARKGLLEQTRRHAAIVSLLGIRHVVLAVNKIDLVDFSQERFDAIVADFEVFAAPLNFRSVVAIPLSARFGDNVTTRSRQTPWYEGPSLIRHLEAVDQAARRGALRFITCGSVDDGKSTLIGRLLHDSIGLLEDQIAGLKDDSRRFGTTGEELDLALLLDGLEAEREQGITIDVAYRFFSTARRAFVVADTPGHAQYTRNMATGASTAELAVLLVDARKGLLEQTRRHAAIVSLLGIRHVVLAVNKIDLVDFSQERFDAIVADFEVFAAPLNFRSVVAIPLSARFGDNVTTRSRQTPWYEGPSLIRHLEAVDTVLDATVVWMSEQALLPGVQYKFKLGARTVTGTVKSVRHVIDIANLLERPASRLDLNEIGRLDIDLEETVHFDSYRENRATGGFILIDRLSNVTVGAGMILDAMVCWFSDETELRVGGDYELRTAAQSGRVRVTGLDYRLDVNTLHRVTDAESLALNDIARVSLSGRQPLMADAYRDNRRTGSFILVDEATNQTVAAGMIR",
    "response#3": "RGAKHFVICGSEGDGKSTLISRLLFENPLEAIEADSKKYGTQGGEVDFAQVAGLTAEGEQGVTVDVVAYRFFSTEKRKFIVADTPGHEQYTRNMVTGASTADAAILMVDARHGILTQTRRHSFLMSLMGMRHVVVAINKMDLVDYSKARFDQIVEEYRAFAKQLGLENITFIPMSALKGDNVIEHSAKMPWYHGTTLLGYLETVELDAEHLQRSPFRFPVQYVNRPNLDFRGFAGTVASGSVSRGDEVVVAKSGRSAHVKRIVSYGGDLQKAVAGQAVTLVLDEEVEVSRGNVLGTLVSGVVRPGDKVVVLPSGRTTKVARLVTQDGDLERAQAGDAVTLVLADEIDVARGDVLAEVADQFEVNLVWMDQEAGHNGRAYWLKSGATRVNATITDIKYKYNINSLEHLSTKNMSLNDIGVATLSLDRSIPFETYAANRTLGSFVLIDRYTNATVAAGMIDHALRRSVNVHAQHVTKEHRRGAKGT"
  },
  {
    "reference": "MASITIRGVKKNYAKTQVVHGVDLDFASGEFVVILGPSGCGKSTLLRMIAGLEEISDGEISIDGTVVNKLEPRERGCAMVFQNYALYPHMSVAQNIGYSLKVAGVPSAERTQRIQAVARTLELEHLLDRKPMALSGGQRQRVAMGRAMIREPKVFLFDEPLSNLDAKLRVQMRSEIRKLHRRLNATSVFVTHDQVEAMTLADRLVVMNGGRVEQVGTPGEIYTRPASRFVATFVGAPAMNILEGTVELDGLSLLGGSRRLAMARTGLPVGTKVAMGIRPEAVRLVAPGIAGALNATVDLVEELGAGRVIYVDLDGAPFSVMTSEAVHPEPGSQVGLKISPDDMHFFSSEAGSRLDVFKASVPEPAL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be designed to have high stability and activity towards ATP + H2O + sn-glycerol 3-phosphate(out) = ADP + H(+) +   phosphate + sn-glycerol 3-phosphate(in).2. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.3. A protein that can perform ABC-type glycerol-3-phosphate transporter activity, ATP binding, ATP hydrolysis activity in ATP-binding cassette (ABC) transporter complex to support carbohydrate transport.4. The protein must exhibit the following characteristics: Part of the ABC transporter complex UgpBAEC involved in sn- glycerol-3-phosphate (G3P) import; Responsible for energy coupling to the transport system..5. The designed protein should contain one or more ABC transporter domains that are essential for its function. The designed protein sequence is ",
    "response#1": "STYGTGCFMLLNTGDQIIHSRNRLLATIAYRLNDTVTYGLEGSIFIAGAAVKWLRDPLHLIEKANDSESMASSVEDTGGVYLVPAFTGLGAPYWDPNARGALFGLTRNTQREHIVRAALEAVCYQSKDLVRAILNDGANLTTLRVDGGMAANNWLLQFLSDILGVNVDRSRCIESSALGTAFLAGLSAGFWSSREEVPGEVVVRVRNVSRGRKVRDVSFSVRAGEVVGLGGLVGSGRTEVARLVFGADKMDSGTVELNGKPLHLSSPREAVRARIGLVPEDRKQQGVILDAPIRINTTLAKIRSISRLGFLDAGKERQVAVALGAEMRLKASSVDAPVSSLSGGNQQKVALAKWFHADCDLLILDEPTRGVDVGAKGEIYNLINDLAKAGKAILVISSEHQELFGICDRVLVMAEGAIVGELTESKFTEQQLLTLAMTRAV",
    "response#2": "STYGTGCFMLLNTGDQIIHSRNRLLATIAYRLNDTVTYGLEGSIFIAGAAVKWLRDPLHLIEKANDSESMASSVEDTGGVYLVPAFTGLGAPYWDPNARGALFGLTRNTQREHIVRAALEAVCYQSKDLVRAILNDGANLTTLRVDGGMAANNWLLQFLSDILGVNVDRSRCIESSALGTAFLAGLQAGVHDVSFDVRAGEILGVAGLVGSGRSNVAETVFGVTPASSGTISIDGKPVSIDSPTTAISHRMAFLTEDRKDTGCLLILNILENMQIAVLQDKYVANGFVQENALSEACEEMCRKLRVKTPHLYERIENLSGGNQQKVLIGRWMLTKPRILILDEPDKCTYGTGSFLIIQAGTQVMRSHHQLLSTVAWSTENSAYYALEGAIFTSGAAIQWLRDGLKIINNAAETETLSRQVTDTKGVYFVPALSGLGAPHWDMNARGAFFGITGGVGREHLVRAVLEAIAYQVKEVVDAINLESTLKVAALKVDGGASQNDFLMQFQADALGVPIERPLILDATAQGAAFGSCF",
    "response#3": "STYGTGCFMLLNTGDQIIHSRNRLLATIAYRLNDTVTYGLEGSIFIAGAAVKWLRDPLHLIEKANDSESMASSVEDTGGVYLVPAFTGLGAPYWDPNARGALFGLTRNTQREHIVRAALEAVCYQSKDLVRAILNDGANLTTLRVDGGMAANNWLLQFLSDILGVNVDRSRCIESSALGTAFLAGLQAGVFKSADKCTYGTGSFLIIQAGTQVMRSHHQLLSTVAWSTENSAYYALEGAIFTSGAAIQWLRDGLKIINNAAETETLSRQVTDTKGVYFVPALSGLGAPHWDMNARGAFFGITGGVGREHLVRAVLEAIAYQVKEVVDAINLESTLKVAALKVDGGASQNDFLMQFQADALGVPIERPLILDATAQGAAFGAVVAI"
  },
  {
    "reference": "MYALAFDLKIEILKKEYGEPYNKAYDDLRQELELLGFEWTQGSVYVNYSKENTLAQVYKAINKLSQIEWFKKSVRDIRAFKVEDFSDFTEIVKS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The design objective is to achieve a protein with strand-helix-helix-strand-strand-helix-helix-strand-helix characteristics.2. The protein must exhibit nuclease activity, RNA binding. The designed protein sequence is ",
    "response#1": "GFWYEIAFASKMGTPGLAHKEEKMGAMVVELKENLLALTTTYYSEDHCVLEKVTATEGDGPAKFQVTRLSGKKEVVVEATDYLTYAIIDITSLVAGAVHRTMKLYSRSLDDNGEALYNFRKITSDHGFSETDLYII",
    "response#2": "ALTDPDNPRWISAQKDIISYVDETEAATSTITKNQDAQNNWLTQQANLSPAPKGFIIAPENGSGVGTAVNTIADKGIPIVAYDRLITGSDKYDWYVSFDNEKVGELQGLSLAAGLLGKEDGAFDSIDQMNEYLKSHMPQETISFYTIAGSQDDNNSQYFYNGAMKVLKELMKNSQNKIIDLSPEGENAVYVPGWNYGTAGQRIQSFLTINKDPAGGNKIKAVGSKPASIFKGFLAPNDGMAEQAITKLKLEGFDTQKIFVTGQDYNDKAKTFIKDGDQNMTIYKPDKVLGKVAVEVLRVLIAKP",
    "response#3": "MTTSRRPSPRIRSFVKDLSATIPGAFRFTRGHYSMEELAREAIIRGADRIVVVGERRGNPGIIRVYAVEGPERPDNIVSFIVKGVSLSRERRWGLPSLRGGEVLVARPLDSGVAVEFADAFVIAFHARLKPPEAAGYVEAVIESLDARTVAVTFRYGGAPVGPMLRLGKR"
  },
  {
    "reference": "MKVAVVGATGLVGTKMLQVLAERNFPVTELLPVASENSVGKEVEFKNNKYKVVGMQEAIDAKPDVAIFSAGGGTSTEWAPKFAEAGITVVDNSSAWRMDPTKPLVVPEINADILTTNDKIIANPNCSTIQMVVALAPLHKAYKIKRIVVSTYQSVTGTGKKAVDQMMGERNKSVAGKETEYPMAYQYPIDLNVIPQIDVFTDNGYTKEEMKMVNETRKIMRDDNIRVTATTVRIPVMGGHSESVNVEFENDYDLAEVKNILEASPGVVVVDDLANAKYPMPKDAHEKDEVFVGRLRRDDTQPNTLNMWIVSDNLRKGAATNAVQIAEYLVANNLV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should be able to enhance the efficiency of 'de novo' L-methionine biosynthetic process, lysine biosynthetic process via diaminopimelate. The designed protein sequence is ",
    "response#1": "LWPLHKACEIKRVVVTTFQSVSGTGTPAIEELRRQSEQVLEKGIEESCRHGLAKDAYPHQIAFNCLPHIDKFEEDGYTKEEHKMINETRKIFHEPKFRLTATTVRVPVFRGHSEAVNIEFKKAISPEEASAILKYAPGVTLIDDVSKNEYPMPIFCEWKDDVFVGRIRADKSVRYGLDMWVVADNILE",
    "response#2": "LKPIYDAVGISRINVATYQAVSGTGKEAIHELVKQLTELLNGRPITSSVYPKQIAFNVLPHIDEFQTNGYTREEMKMVWETQKIMEDDSLMVNPTTVRVPVLYGHSEAIHLELKSPLTSDDARALLKKAPGVKVIDNPSKLEYPTPITHASGHDEVFVGRIRQDITHPTGLNMWVVADNIRL",
    "response#3": "LWPLHKACEIKRVVVTTFQSVSGTGTPAIEELRRQSEQVLEKGIEESCRHGLAKDAYPHQIAFNCLPHIDKFEEDGYTKEEHKMINETRKIFHEPKFRLTATTVRVPVFRGHSEAVNIEFKKAISPEEASAILKYAPGVTLIDDVSKNEYPMPIFCEWKDDVFVGRIRADKSVRYGLDMWVVADNILD"
  },
  {
    "reference": "MFTNYSEKGMPLVRQDSEMTAKYAIVLAAGQGTRMKSKLYKVLHPVCGKAMVDHVLTELEKDQIDEIVTIVGHGAAKVEETLGKRTKYALQAEQLGTGHAVLQAEELLGDKQGMTLVTCGDTPLFTAKTFAKLFEQHEQSGAVATVLTAKTDEPFGYGRVIRNHRDEVEKIVEQKDTTPEEALVNEINTGVYCFDNQALFKTLHEVKNDNAQGEYYLPDVIEILKAKGRPVGAYCMDEFEESMGVNDRIALAKATKVMQRRINELHMKNGVTLIDPATTYIEADVKIGADTVIEPGVSLKGKTVIGEDCVIGAHSELRDTVLEDGIEVKASFLEGAIMRNGSNIGPYSHLRPQADIGEGVHIGNFVEVKKATLGKNTKVGHLTYVGDATLGKEINVGCGTVFVNYDGQNKHHTNVGDYSFIGSGSNIIAPVEIADHAYVAAGSTITEDVASREMAIARGRQVNKPGYYDRYPVAKAAAESENKD",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should be able to enhance the efficiency of peptidoglycan biosynthetic process.2. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+) binding. The designed protein sequence is ",
    "response#1": "KMTFEQAGLAQVKYKAITRAQVWSNPCVFPKLCDEIEAALGYPAFVKPANLGSSVGIAKVRSRQELEAALDNAASYDRRLVVEAGVVAREVECAVLGNDQPQASVVGEISYDSDFYDYETKYTEDRADLLIPAPIPDAIARQIQDMALQAFAAVDAAGLARVDFFYVEATQEVLINEINTLPGFTATSMYPQLWAHSGVSFPELVDRLIG",
    "response#2": "FEQAGLAQVKYKAITRAQVWSNPCVFPKLCDEIEAALGYPAFVKPANLGSSVGIAKVRSRQELEAALDNAASYDRRLVVEAGVVAREVECAVLGNDQPQASVVGEISYDSDFYDYETKYTEDRADLLIPAPIPDAIARQIQDMALQAFAAVDAAGLARVDFFYVEATQEVLINEINTLPGFTATSMYPQLWAHSGVSFPELVDRLIG",
    "response#3": "FEQAGLAQVKYKAITRAQVWSNPCVFPKLCDEIEAALGYPAFVKPANLGSSVGIAKVRSRQELEAALDNAASYDRRLVVEAGVVAREVECAVLGNDQPQASVVGEISYDSDFYDYETKYTEDRADLLIPAPIPDAIARQIQDMALQAFAAVDAAGLARVDFFYVEATQEVLINEINTLPGFTATSMYPQLWAHSGVSFPELVDRLIG"
  },
  {
    "reference": "MVRNKYSLNAGKSPSKKSRTNSAKQHDASDNEGGGGGEGGSSGGTLTGTETGTPQRCDTVHKQNLYIISFPVIFVFNVVRSLLYQLFIIFRYIYNFTTKVVYRPVKKDCGLEIVINHQQQQQEAPSHLQHDPQLDNPLVHQYDPQQHHPLQYSSSSGVLVNAEGREMSVQRSASGSQVGPGDPLLAKQKHHHRRAFEYISKALKIDEENEGQKELAIELYRKGILELERGIAVECWGGRGEVWERAQRLHDKMQTNLSMARDRLHFLECMLEANKLEISEAHKLQRSSTFTLSVGQPVQHLPTPAAATQPARPASNATTTAKQAKFKVPHPVKGGALSRKGQQQQQQQENRVMTYGDSNGNGVGGGRKAPEVEASGRKLTVGYKRPGNLGVMNKSQTLPRSMGGSRTTPTGGSGVPAGTGYGGVGPANAGAGVAAGCTAGGMPKIVPKPAATPPAIRRQFSIPSGSPIRKASNGYGHSGGKNTPPRSKTPLGTAPSGNQSGGQQQPQIAVKGVEPKLVQIIMDEIVEGGAKVEWQDIAGQEVAKQALQEMVILPSVRPELFTGLRTPAKGLLLFGPPGNGKTLLARAVATECSATFFSISAATLTSKYVGDGEKLVRALFSVAREMQPAIIFIDEVDSLLSERSSGEHEATRRLKTEFLVQFDGLPANSEADKIVVMAATNRPQELDEAALRRFPKRVYVTLPDLDTRELLLRRLLEKQNSPLDDADLKRLAMLTEGYSGSDLTALAKDAALEPIRELNVEQVKNMDP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the microtubule organizing center following nucleation.; ATP-dependent microtubule severing protein2. The protein contains novel MIT domains that confer a unique function or activity.3. We are looking for a protein with broad substrate specificity for n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1)   alpha/beta tubulin heterodimers..4. The protein should have Helical domains to ensure proper insertion into the membrane.5. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.6. The ATP binding site should be located in a region of the protein that is accessible to the ligand.7. A protein that contributes to positive regulation of microtubule depolymerization, protein hexamerization, microtubule severing by functioning in membrane, microtubule, spindle, centrosome, cytoplasm with isomerase activity, microtubule binding, microtubule severing ATPase activity, ATP binding. The designed protein sequence is ",
    "response#1": "DIADLEEAKKLLKEAVVLPMWMPEFFKGIRRPWKGVLMVGPPGTGKTLLAKAVATECKTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPTTIFIDEIDSMCSRRGTSEEHEASRRVKAELLVQMDGVGASENDDPSKMVMVLAATNFPWDIDEALRRRLEKRIYLSCDVDLVKIAQKMEGYSGADITNVCRDASLMAMRRRIEGLTPEEQKEHHRKAFDLISKALKIDEENTGNKDVAIEFYKKGISELDKGIAVNCYYGSGEKWERAQRLQEKMKNNLKMAKERLSQHQQKHHHRRAFEYISKALKIDEENEGQKELAIELYRKGILELERGIAVECWGGRGEVWERAQRLHDKMQTNLSMARDRLHELLWETYGVSSSTEISENVKLAREYALLGNYSSAVVCYEGVSEQIKKYLFSVRDSSLQQKWQQVWQEISEESKHVRDIMRGAPWKGVLMVGPPGTGKTLLAKAVATECRTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPTTIFIDEIDSMCSRRGTSEEHEASRRVKAELLVQMDGVGGTSENDPSKMVMVLAATNFPWDIDEALRRRLEKRIYIPLPKEELQMPVTMEDFNLTLKKISKSVSVADLEKYEAWMSEFL",
    "response#2": "DIADLTEAKRLLEEAVVLPMWMPDFFKGIRRPWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPSTIFIDEIDSLCSRRGSDSEHEASRRVKSELLVQMDGLGSATDEPAKVVMVLAATNFPWDIDEALRRRLEKRIYLFDLDFDEISSLLHGYSGADVANVCRDASMMSMRRRIAEIGTDEILQKQHHKKAFDFISKALKYDEENEDFKEMSIDLYRKGIEELQKGIAIDFSKGQGTTWERAHRLSDKMKVNLEMARDRLDKAKRKAEEEKLKEAVQIARDNALLGLYSEALSCYDSAIDCIRAYLQTGLSVNLQSSWSSALQTLENEADRVNSIVAI",
    "response#3": "MAHHLREARQSALYGNYDGSLTFYEGVMMEIEQLIQLNYEDPKLKINKLNLIKYRKMIEAEHDQVKEYQLHEISENVKLAREYALLGNYTSAMVCYQGVLEQIKKYVFSVRDAALQQKWQQVWQEIGQESKNVREIMNKLKESQRHGEEAEASLNQMETRQKEVEKDLEDTKRLQQQLEKQLADQQQVTAQRRLQLQKLEQDLAGEQQALADCERELELQKKTTCSHHLREARQSALYGNYDGSLTFYEGVMMEIEQLIQLNYEDPKLKINKLNLIKYRKMIEAEHDQVKELLLFGPPGNGKTMLAKAVASESNATFFNISAASLTSKYVGEGEKLVRALFSAARELQPSIIFIDEIDSLLCERREGEHDASRRLKTEFLIEFDGVQSSGDDRVLVMGATNRPQELDEAVLRRFTKRVFVVLMVGPPGTGKTLLAKAVATECKTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPTTIFIDEIDSMCSRRGTSEEHEASRRVKAELLVQMDGVGASENDDPSKMVMVLAATNFPWDIDEALRRRLEKRIYIPLPTDEGRYLLLFGPPGNGKTMLAKAVAMESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEIDSLLCERREGEHDASRRLKTEFLIEFDGVQSSGDDRVLVMGATNRPQELDEAVLRRFAKRIYVLPTNKGIYDLFKRVASNELDTHELTKLAQMTEGYSGSDLTALAKDAALGPIRELG"
  },
  {
    "reference": "MSLAPHGGTLVNRVDESYDVSGIQKEIELDLISFADLELIGIGAYSPIEGFFNEKDYVSVVENMRLSSGVVWSLPITLPVDAQKAAELSLGETVKLTYEGETYGVIQIEDLYVPDKQKEAVNVYKTDEQEHPGVKKLFSRGNTYVGGPITLIKKASKQFPEFTFEPSETRRQFAEKGWETIVGFQTRNPVHRAHEYIQKTALETVDGLFLNPLVGETKSDDIPADVRMESYQVLLDHYYPKDRVFLGVFLAAMRYAGPREAIFHALVRKNYGCTHFIVGRDHAGVGDYYGTYEAQELFDTFKPEELGITPLKFEHSFFCKKCGNMGTAKTCPHGREHHVILSGTKVRGMLRDGVLPPAEFSRKEVVEVLIKGMKKKEEVGVS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. We require a protein that can specifically bind to the metabolites produced in the Sulfur metabolism, hydrogen sulfide biosynthesis, sulfite from sulfate: step 1/3 pathway.2. The designed protein must be optimized for ATP binding. The designed protein sequence is ",
    "response#1": "LRFITCGSVDDGKSTLIGRLLHDSKMIYEDQLQALTRDSANQGTTGGDIDFALLVDGLEAEREQGNTIDVAYRFFTTPRRSFMVADTPGHEQYTRNMATGASNAQLAIILIDARKGVLVQTRRHSFICSLLGIRHVLLAVNKIDLVDYRKDVFDGIVHDYLAFASELGFTSIIPVPISARYGDNIDGRPHGGKLVNRIVSSKRRTALLKETHELPSIEISYERLIDLLDIANGAFSPLEGFMVQEDYLHVLYDMRLSNDLPWTIPVILDVDPEEISNVKEGDDIALKYKDEIYAIMRVEEIYGWDKKEYARQVYKTTDPNHPGVAKTYQRKELLLGGTIDLLKDQLRFITCGSVDDGKSTLIGRLLHDSKMIYEDQLQALTRDSANQGTTGGDIDFALLVDGLEAEREQGNTIDVAYRFFTTPRRSFMVADTPGHEQYTRNMATGASNAQLAIILIDARKGVLVQTRRHSFICSLLGIRHVLLAVNKIDLVDYRKDVFDGIVHDYLAFASELGFTSIIPVPISARYGDNIVKRSGNTDWYDGPPLLDYLESVEVGSHG",
    "response#2": "SLLRFITCGSVDDGKSTLIGRLLYDSRMIFEDQLAALEADSRKMGTQGQNIDFALLVDGLAAEREQGITIDVAYRFFATEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARRGVLTQTRRHSFLASLVGIRNIVLAINKMDLVDYSEDVFNSIIQEYKSFASGLSFESIVPIPMSALAGDNIRELLRFLTAGSVDDGKSTLIGRLLHDSDQVYDDHLATLRRDSQRLGAAGGATDFALLTDGLRAEREQGITIDVAYRYFATERRKFIIADCPGHAQYTRNMVTGASTCDLALVLVDARHGVVEQTRHHAVIAALLGISHVVVCVNKMDLVDYDEAVFTRIRADYASFAARLETRDVHFLPVSALAGDNVVARSERMPWFCGEPLLSYLEGVHIAADRNLAKKEPWYRLPFILPVQRVNRPNLDFRGYSGTIASGNIKVGEEIRTVPSNQKAKVKELFIGDKSIKSSSNKQSITLTLNKEIDISRGDILCVKENPPTVEVSDQFEVTLVWMNDAAMQPGRSYLLKLGAKTVTATVTALKHKVNVNTLEQTTAEHLELNEIGVCTLSLSQPVAFDSYEKNRETGGFILIDRFTNDTLAAGMIREK",
    "response#3": "RELLRFLTAGSVDDGKSTLIGRLLHDSDQVYDDHLATLRRDSQRLGAAGGATDFALLTDGLRAEREQGITIDVAYRYFATERRKFIIADCPGHAQYTRNMVTGASTCDLALVLVDARHGVVEQTRHHAVIAALLGISHVVVCVNKMDLVDYDEAVFTRIRADYASFAARLETRDVHFLPVSALAGDNVVARSERMPWFCGEPLLSYLEGVHIAADRNL"
  },
  {
    "reference": "MIQAGLRIDVDTFRGTRDGVPRLLELLDEAGLKATFFFSVGPDNMGRHLWRLARPAFFWKMLRSRAASLYGWDILLAGTAWPGKPIGRELGPLMRRTQAAGHEVGLHAWDHHAWQTHAGVWSVQQLGEQIRRGSDCLADILGQPVRCSAAAGWRADGRVVEAKQPFGFRYNSDCRGRGAFRPRLADGSPGIPQVPVNLPTFDEVVGPGLPREAYNDFILERFAAGRDNVYTIHAEVEGLLLAPAFRELLRRAERRGIRFRPLGELLPDDPRSLPLAELVRGRLAGREGWLGVRQP",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Catalyzes the deformylation of 4-deoxy-4-formamido-L- arabinose-phosphoundecaprenol to 4-amino-4-deoxy-L-arabinose- phosphoundecaprenol.2. The designed protein have NodB homology domains with specific sequence motifs that are crucial for its activity.3. We require a protein that can specifically bind to the metabolites produced in the Bacterial outer membrane biogenesis, lipopolysaccharide biosynthesis pathway.4. The protein design must be focused on enhancing the efficiency of lipopolysaccharide biosynthetic process. The designed protein sequence is ",
    "response#1": "VPNLWEILNKKKIKATFFFTVGPDNMGRHFWRLIKPKFLFKMFRINPFKIYGLNILRSGFIGKGKNIGKICKNEIKSASKDHEIGLHAWDHFSWQTWINMFSKKKIIKHINLGKNALQHIIKYPVTCFASPGWRTNEYVLRTLN",
    "response#2": "VPNLWEILNKKKIKATFFFTVGPDNMGRHFWRLIKPKFLFKMFRINPFKIYGLNILRSGFIGKGKNIGKICKNEIKSASKDHEIGLHAWDHFSWQTWINMFSKKKIIKHINLGKNALQHIIKYPVTCFASPGWRTNEYVLRTLG",
    "response#3": "VPNLWEILNKKKIKATFFFTVGPDNMGRHFWRLIKPKFLFKMFRINPFKIYGLNILRSGFIGKGKNIGKICKNEIKSASKDHEIGLHAWDHFSWQTWINMFSKKKIIKHINLGKNALQHIIKYPVTCFASPGWRTNEYVLRTLK"
  },
  {
    "reference": "MLDLSLPSGLRDLLPDHSAHLAELSSKLHDVFSRFGYRRVFLPTLERLDVVERGLSPAALADVMKFVEPGSGEVVAIRPDITPQIARLYAARPDALPSPARLCYDGPVLRAREARAGRPREVYQAGVELLGAGGASADAEALVLLARSLERVGLKAPRVEVGHARFAEAVMEAARLPERLRSAAWEALSRKDRAALAAAAAKGRGSAEAREAVPQLAGLFGDGALDRARAIARAVPEAAAPLAETEAALRIARRRGVREVAVDLGEARGLGYYTGITFAGYAPGAGAAVARGGRYDGLLARFGRPGPAIGFAVDLEFATQALERVNGRGRGVRPRRASARGGRARARPR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in histidine biosynthetic process.2. Prioritize Basic residues compositional bias within the protein sequence to achieve desired characteristics.3. A protein that is capable of catalyzing reactions within the Amino-acid biosynthesis, L-histidine biosynthesis pathway with high efficiency and specificity.4. The protein must exhibit the following characteristics: May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine.. The designed protein sequence is ",
    "response#1": "MRENFKELPMGVRDYLPGEAALRRYLENLFVELVAKEGFQEVITPSFEYYETLRESSETDLIKFVDRTGELLALRGDMTTPIARVVSTKMKHRELPLKIFYAATVFSGNFSGRNKLREFKQLGIEVFGDITSKLESDLIKLMAVYLRKAKVKNLEISLGHVDILTGLIEEYELFNHFSEIKNALNQKDYVALEGIFERAGKSQRVRESLVDFLQQRGGKAILRKAKKMLSHPKAKKALEELEEVTEYLISEGVRELKLDFSLVRDLNYYTGLVFEAYTPYLGYPLGGGGRYDTLLKKFGWDTPAFGFALGLERIL",
    "response#2": "GIEDLLPEEAKKLEFYRRQLLDGFELSGYDLVLPPIAEFTDSLLTGKGRHLAVDTCRFTDQESGKMMGVRADMTPQVARIATNRIKQTGIQRLCYVGEVLKTRNNKAKGSRSPIEIGAELFGHSGVESDIEVIELMVSSLHNIGLQDLTLSLGHVSVVDELMNVSGLTPTQKENLVDILLRKAVPEYTDFVAELTVTEAQKQAFELLLSLCGDIATVTEAMQGLAGLSEPMQRHLAHLQSVVDHFAGFDGLKIHMDLADLRGYQYHTGMIFSCYAAGRKMYQLARGGRYDGIGSEFGSAQLATGFSLDLL",
    "response#3": "MRENFKELPMGVRDYLPGEAALRRYLENLFVELVAKEGFQEVITPSFEYYETLRESSETDLIKFVDRTGELLALRGDMTTPIARVVSTKMKHRELPLKIFYAATVFSGNFSGRNKLREFKQLGIEVFGDITSKLESDLIKLMAVYLRKAKVKNLEISLGHVDILTGLIEEYELFNHFSEIKNALNQKDYVALEGIFERAGKSQRVRESLVDFLQQRGGKAILRKAKKMLSHPKAKKALEELEEVTEYLISEGVRELKLDFSLVRDLNYYTGLVFEAYTPYLGYPLGGGGRYDTLLKKFGWDTPAFGFALGLERIS"
  },
  {
    "reference": "MIKATKLKKKYDKLKIAKWSFLGILIILFILSNHYYIKHSNIFQNILLTSLTILSTGLIFLTKSGKKFLIFTKSAIHETKLITWPNFKDTLHVTFTVIIVTILLALILWGLDNILIWFISLITSLRL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have protein transmembrane transporter activity within plasma membrane to regulate protein secretion, intracellular protein transmembrane transport, protein transport by the Sec complex, protein targeting. The designed protein sequence is ",
    "response#1": "EDVLKKFGKYELIRKETISGVVSEELQKKAVFAILTALGGILLYLGVRFQPVWGFGAILALAHDVITVLGAYSITQREVNLEVVSAILVVAGYSVADTVVIFDRIRENLRKKKGFTLEEIMDLSINQTLSRTIMTSLTTLVTALTLFILGGYALSNIMFAFVVGVVVGTYSSVFVASAFVLDMQS",
    "response#2": "NSTQLPISQIVQFPNEQSLIDSYKSNPQSITYGISFNNIDYQNNILDYNILLNSTFQNTQLISIVQNLFLSTFAEIKGITTTTTTTLSSIEEFPNLTSSVSVSSYLYVIYLPLLFLFSLQQLLVTSVTERKNHIKEVMKVMGLKELVYWVTIIIVQTITNVINILLVMVVLYFTKTLTTSLNPVMLFLQFLLYSFSMVAIGIILSNLVDTPKTASAISSFLLLILVGVSCFYQFYLKSKTSSSWLRSILFLFSPCAFGEFLYKMGNDQQMMLTTNWSDPQVKISFLFLIIDIFLYFTIAWL",
    "response#3": "NSTQLPISQIVQFPNEQSLIDSYKSNPQSITYGISFNNIDYQNNILDYNILLNSTFQNTQLISIVQNLFLSTFAEIKGITTTTTTTLSSIEEFPNLTSSVSVSSYLYVIYLPLLFLFSLQQLLVTSVTERKNHIKEVMKVMGLKELVYWVTIIIVQTITNVINILLVMVVLYFTKTLTTSLNPVMLFLQFLLYSFSMVAIGIILSNLVDTPKTASAISSFLLLILVGVSCFYQFYLKSKTSSSWLRSILFLFSPCAFGEFLYKMGNDQQMMLTTNWSDPQVKISFLFLIIDIFLYFTIAWL"
  },
  {
    "reference": "MAELTIRPEEIRDALENFVQSYKPDAASREEVGTVTLAGDGIAKVEGLPSAMANELLKFEDGTLGLALNLEEREIGCVVLGEFSGIEEGQPVSRTGEVLSVAVGEGYLGRVVDPLGNPIDGLGEIETSGRRALELQAPTVMQRKSVHEPMETGYKAVDAMTPIGRGQRQLIIGDRQTGKTALAVDTIINQRDNWRTGDPNKQVRCIYVAIGQKGSTIASVRGALEENGALEYTTIVAAPASDPAGFKYLAPYTGSAIGQQWMYEGKHVLIIFDDLSKQADAYRAVSLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDAEGAGSMTGLPIVETKANDVSAFIPTNVISITDGQCFLESDLFNAGQRPALNVGISVSRVGGSAQHKAMKQVSGRLRVDLAQFRELEAFAAFGSDLDAASKSQLERGQRMVELLKQNQYQPMSTEDQVVSVWAGTTGKMDEVPVADIRRFEKELLEYLHRQEQGLMTSIREGGKMSDDTLQAVAEAIAAFKKQFETSDGKLLGEDAPSAAK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have a high affinity for ATP.2. The protein should have a low Km value for ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate.3. A protein that can perform proton-transporting ATP synthase activity, rotational mechanism, ATP binding, proton-transporting ATPase activity, rotational mechanism in proton-transporting ATP synthase complex, catalytic core F(1), plasma membrane to support proton motive force-driven ATP synthesis. The designed protein sequence is ",
    "response#1": "GQRVIDAFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPELIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVSPPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYL",
    "response#2": "GIKAVDAMIPIGRGQRELIIGDRQTGKTSIAIDTIINQRSNYEAGNPVYCIYVAIGQKGSTVAALVNTLQEKGAMDYTIVVSATASDPAAMQYFAPFAGAAIGEYFRDSGRHALVVYDDLSKQAVAYREVSLILRRPSGREAYPGDIFYLHSRLLERAAKIINQPEVAREMNDLPESMRDKVKGGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLETDLFNQGNRPAINVGISVHGWLKENINEDWIKLRNMAMELLKEEDRLQEIVKLVGEDVLPDNQRLILEVARLIKIGFLQQNAFSKVDRFATPEKQYWMLKIIMFLYEKARPLIKNGWLKENINEDWIKLRNMAMELLKEEDRLQEIVKLVGEDVLPDNQRLILEVARLIKIGFLQQNAFSKVDRFATPEKQYWMLKIIMFLYEKARPLIKNK",
    "response#3": "VPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGXVVSIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVV"
  },
  {
    "reference": "MHRISSAFEADLHDVAATRRIEHRTAAGLPAHALMQRAGLAVARLAGALAPHARSIWIACGPGNNGGDGLEAAAWLRRRGVDVLVTWLGDERRLPPDAAASLARARAAGVVFAARPPDNYDLAIDALLGIGATRPPEGAMAEWLWRMSEGPAPVLCVDVPSGLDADTGHLVAGPTAATPQAAGSRRHCLSLLTLKPGLFTAHGRDAAGEVWFDDLDCPPGDEAPVARLAGAPAPRMRAHATNKGSHGDVAVVGGAAGMAGAPLLAATAALHAGAGRVFVAPLDGKASMLDASRPELMFRRLVDIRLETVAVVCGCGGGDAVRTVLPRILAQTPTLVLDADALNAVAGDASLRAAVRRRAARGWRTVLTPHPLEAARLLGTDVGEVQADRLAAALRLALDYGCVAVLKGSGTVVAAPDETIRINPTGNGLLATAGTGDVLAGMVGAALAAGRPALEAACAAVWRHGDIADRWPAHQPLTAGALAMAV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must have high specificity towards ATP binding.2. The protein should be designed to have high stability and activity towards (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.3. The designed protein should have a high specificity for K(+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "GLIAGSDLYPGAAQLTAKSAVNCGVGLLRTHVPESVRPLVAASVPESVPLDDEQIKEALSSSRRHLHGKGAKISAWAIGPGLDGRTPPTGVIAEVFASRQPAVIDASGLEVVPTGPSEQPRVLTPHSKELRMLLGRAGVKVTPDEIEQDPIRWTRWAALAYNAVVLLKGPTTYIVAPNGTSLVVSHSAPQLATAGSGDVLTGILGYLLSDNSLTGTQGLRTVPNRRIMELAACAAIIHGHLGRVAAEDGTVSASRLIDNC",
    "response#2": "GLIAGSDLYPGAAQLTAKSAVNCGVGLLRTHVPESVRPLVAASVPESVPLDDEQIKEALSSSRRHLHGKGAKISAWAIGPGLDGRTPPTGVIAEVFASRQPAVIDASGLEVVPTGPSEQPRVLTPHSKELRMLLGRAGVKVTPDEIEQDPIRWTRWAALAYNAVVLLKGPTTYIVAPNGTSLVVSHSAPQLATAGSGDVLTGILGYLLSDNSLTGTQGLRTVPNRRIMELAACAAIIHGHLGRVAAEDGTVSASRLIDNR",
    "response#3": "PTAHKMDVGAVLVIAGSFGMTGAAAMAATAAYRAGAGLVRLALPASLTAALNAQLTEVVFRPMSETRAGTLSFHGFQRLLDEVEGTRAVLLGPGLSRHPATAHLIRRLVPLIHAPLVVDADALTAMVGHDRLWRQREKPTVITPHPGEMSRLLGQPATVLEEDRIAAAKEAARRFNATVVFKGSPVVIAAPDGRTFVNPTGTPALAVVGTGDVLAGMIAALIAQRVEVAHAAALACFTGGLAAQRMTAQLGLRGFTALDLIAAIL"
  },
  {
    "reference": "MALISTRRTLSTLLNKTLSSSTSYSSSFPTLSSRSRFAMPLIEKVSSSRTSLGPCYISTRPKTSGSGYSPLNDPSPNWSNRPPKETILLDGCDYEHWLIVMEFTDPKPTEEEMINSYVKTLTSVLGCEEEAKKKIYSVCTSTYTGFGALISEELSCKVKALPGVLWVLPDSYLDVPNKDYGGDLYVEGKVIPRPQYRFTEQRHTRPRPRPRYDRRRETMQVERREPSMGLHSPVNPGEFNKPSA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Involved in organellar RNA editing The designed protein sequence is ",
    "response#1": "TFHDQIAMLSHRCFNALTNSFQPSLLGRKILAAIIMKRDPEDMGVVVSLGTGNRCVKGDSLSLKGETVNDCHAEIISRRGFIRFLYSELMKYNHHTAKNSIFELARGGEKLQIKKTVSFHLYISTAPCGDGALFDKSCSDRAVESTESRHYPVFENPKQGKLRTKVENGEGTIPVESSDIVPTWDGIRLGERLRTMSCSDKILRWNVLGLQGALLTHFLQPVYLKSVTLGYLFSQGHLTRAICCRVTRDGKAFEDGLRYPFIVNHPKVGRVSVYDSKRQSGKTKETSVNWCMADGYDLEILDGTRGTVDGPGKELSRVSKKNIFLQFKKLCSFRARRDLLQLSYGEAKKAARDYDLAKNYFKKSLRDMGYGNWISKPQEEKNFC",
    "response#2": "VLADAVSRLVLGKFSDLTDNFSSPHARRKVLSGVVMTTGTDVKDAKVISVSTGTKCINGEYMSDRGLALNDCHAEIISRRSLLRFLYAQLELYLNNKEDQKKSIFQKSERGGFRLKDTVQFHLYISTSPCGDARIFSPHEPVLEGMAPDSHQLTEPADRHPNRKARGQLRTKIESGEGTIPVRSNASIQTWDGVLQGERLLTMSCSDKIARWNVVGIQGALLSIFVEPIYFSSIILGSLYHGDHLSRAMYQRISNIEDLPPLYTLNKPLLSGISNAEARQPGKAPNFSVNWTVGDTAIEVINATTGKDELGRPSRLCKHALYCRWMRVHGKVPPHLLRTKITKPTTYHESKLAAKEYQAAKARLFTAFIKAGLGAWVEKPTEQDQFL",
    "response#3": "SLGTGNRCVKGDSLSLKGETVNDCHAEIISRRGFIRFLYSELMKYNHHTAKNSIFELARGGEKLQIKKTVSFHLYISTAPCGDGALFDKSCSDRAVESTESRHYPVFENPKQGKLRTKVENGEGTIPVESSDIVPTWDGIRLGERLRTMSCSDKILRWNVLGLQGALLTHFLQPVYLKSVTLGYLFSQGHLTRAICCRVTRDGKAFEDGLRYPFIVNHPKVGRVSVYDSKRQSGKTKETSVNWCMADGYDLEILDGTRGTVDGPGKELSRVSKKNIFLQFKKLCSFRARRDLLQLSYGEAKKAARDYDLAKNYFKKSLRDMGYGNWISKC"
  },
  {
    "reference": "MTKRAEAKYKIDRRMGQNIWGRSKSPVNRREYGPGQHGQRRKGKLSDFGTQLKAKQKLKGYYGNISEKQFRKYYAEAIRMKGDSGDNLIGLLERRLDAVVYRAKFVPTVFASRQFINHGHIKVNGRRVNIASYQVRVGDVIEVKEASRQLTLVLEASQLAERDVPEYYDVDHGKMSAKVTRIPLPNEVPYPVIMEPNLVIEFYSR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should contain one or more S4 RNA-binding domains that are essential for its function. The designed protein sequence is ",
    "response#1": "NGAQAFVSAQTSTIPKTAFTAHDFVDYTLPQEQKGKEQKEQQWMKNSQTKDTDVHPYADFAVLEILLFPDSSTDRKIFNHFIQPAIRAYKQLGDSLNIFANQTLDQPKHNRYYLLGYPFLRNKAASLFLNQTEQRKEHMDKTTQILHEKPFVVLTDPAKPTLIRNKGANFTGNTWTSNYDLSKKSGLYHKFLGKKYQIYGKSIVISDLNLSSGSSGSLLLNDRKQIVGIYFR",
    "response#2": "NGAQAFVSAQTSTIPKTAFTAHDFVDYTLPQEQKGKEQKEQQWMKNSQTKDTDVHPYADFAVLEILLFPDSSTDRKIFNHFIQPAIRAYKQLGDSLNIFANQTLDQPKHNRYYLLGYPFLRNKAASLFLNQTEQRKEHMDKTTQILHEKPFVVLTDPAKPTLIRNKGANFTGNTWTSNYDLSKKSGLYHKFLGKKYQIYGKSIVISDLNLSSGSSGSLLLNDRKQIVGIYFG",
    "response#3": "NGAQAFVSAQTSTIPKTAFTAHDFVDYTLPQEQKGKEQKEQQWMKNSQTKDTDVHPYADFAVLEILLFPDSSTDRKIFNHFIQPAIRAYKQLGDSLNIFANQTLDQPKHNRYYLLGYPFLRNKAASLFLNQTEQRKEHMDKTTQILHEKPFVVLTDPAKPTLIRNKGANFTGNTWTSNYDLSKKSGLYHKFLGKKYQIYGKSIVISDLNLSSGSSGSLLLNDRKQIVGIYFS"
  },
  {
    "reference": "MLVTISGPPGSGKSTVASTLADHLSYEHISGGDIFRGLAEDRDLTLAEFNELAEEDPQIDKDLDRRLRETARGRDDIVLESRLAGWMAGEYADIKIWLDAPMSVRARRIAEREDKTPAAARAETEAREESETQRYEEYYAIDFDDITIYDLSVNTARWGPDAVTDIVLDAVDAYQAERDEGPTPIENVRYQF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be able to bind ATP ligand in a variety of conditions2. We need a protein that can perform ATP + dCMP = ADP + dCDP with high efficiency.3. The designed protein should be involved in pyrimidine nucleotide metabolic process, phosphorylation by localizing to cytoplasm and exhibiting CMP kinase activity. The designed protein sequence is ",
    "response#1": "IVAIDGPSGTGKSSTSKAVAAKLGLSYLDTGAQYRAITWWMVSNGIDITDPTAIAAVAGKPEIISGTDPSAPTITVDGTDVAAPIRTQEVTSKVSAVSAVPEVRTRITELQRSLATSAENGIVVEGRDIGTTVLPDADLKIFLTASPEARAARRSGELKGADVNSTREALLKRDAADSSRKTSPLAKADDAVEVDTSHLDIEASIEQVKQIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYLALTNGIEVTDQNRIVDLLAQYPIRFGRDENGQQLVFVGDEDVTLPIRDNQVTNNVSAVAALPLVREELVRLQQDIAQAGGIVMDGRDIGTVVLPQAELKIFLVASVEERALRRFKENTERGIETDLESLKEEIAARDYKDSNREVSPLKAADDAITFDTSGLTVEQSVEQVTALLNR",
    "response#2": "ITVDGPSGAGKGTLCQALAEVLGWQLLDSGAIYRVLALAAIHHQVDIQSEDALVPLAANLDVRFVPRKGKLSVMLEGEDVSNEIRTEAVGNTASQAAAFPRVREALLRRQRAFRVAPGLIADGRDMGTVVFSDAPVKIFLDASAEERARRRMLQLQEKGFSVNFECLLSEIQERDYRDRNRAVAPLAPAKDALILDSTSMSIEEVIDKALNYAAV",
    "response#3": "VIAVSGQPGSGKTTIAREIARVLGLPLVSSGLLFREMAARMGMDFIEFHKYAETNPDIDKKVDSLAIERAKAGDVVLEGHLTAWIVRPYADVCIYLKASLETRARRVALRDGKSLQDALREVAEREELNRRRYLSIYGIDINDLSIFDLVLDTR"
  },
  {
    "reference": "MRSRVPKVLHPICGRPMIDWVLQAVTEAGAKDVKVIANPHHAEVAAHLDGKAEVVYQRDPKGTAHALLQIPADELEKMQVLVVNGDSPLLTAASILNLVHQHQEAGAPATIASVEDPTRDDGRIIRAADGSLERIVERKDATPELRQTVHEFNVGLYCFDGARLAEALAKVTDDNKAGEFYLTDVFLHMKPVTVVKLSDPGEAMGVKDRIRLAKAIQVLRTRLLEQCMAGGVTIVDPASTFIDADVTVGEDTVIEPFTVLKGHTAIGAECRIGPHVYIEDARIGDRSDCGPFVKVRPGTEIAEDVHIGSFAELVRTKVGRGSRVPHVSYLGDTDLGEDANIGAGTITANFDGTKKNRTQIGDGAFVGVDTMLVAPVKLGKGSRTGAGSVVTKDVPDGATAVGVPARVIRRKQVSADRNSDSRSGTR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that is capable of catalyzing reactions within the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway with high efficiency and specificity.2. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).3. The designed protein should be able to compete with endogenous proteins or inhibitors for UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA, Mg(2+) binding.4. The ideal protein would be one that plays a critical role in lipid A biosynthetic process, UDP-N-acetylglucosamine biosynthetic process, regulation of cell shape, cell morphogenesis through magnesium ion binding within cytoplasm.5. The protein with the reaction acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate, the Proton acceptor must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLGFEASDPTGYGRLIRENGTVTGIVEHKDATPAQREICEINTGVMVLPSAKLAGWLARLDNKNAQGEYYLTDVIAMAVSDGVAVHTAQPAAALEVEGVNDRVQLAALERAHQFNVAERLMRAGVTLADPGRFDLRGSLQCGRDVFIDVGCVFQGTVELADGVRIGPHCVITDAVIGPGTQVEAFSHVEQARVADQARIGPYARLRPGADIGPHAHVGNFVEIKKSVLGKGSKANHLAYIGDAQIGERVNIGAGTITCNYDGVNKFQTIIEDDAFIGSDSQLVAPVRVGKGATLGAGTTLSRDAPAGQLTLSRSTQRHISNWQRPVKIKK",
    "response#2": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLTGLPDPSGYGRVVRDAQGNVLRIVEQKDASPEELAVHEINTGILVAPTRFLKRWIASLRNDNAQGEYYLTDIIAMAVADGVPVVTAQPETEVEVLGVNSKVQLAELERWHQRQIAQGLMEQGVSLINPANTYIGVDVKIGSDTVIQPGVMLEGNTVIGEDCHIGPNSRITNSTIGDRTKVDSSVVDSSVVGSDVAVGPFAHIRPATNVGNRAKVGNFVELKKTTLGEGSKASHLTYLGDAEIGTDVNIGCGTITVNYDGVNKFKTIIGDRSFIGCNANLVAPVTINSDTYVAAGTTITKEVPPFALAIGRSRQRNIDGWKRPVKK",
    "response#3": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVFVEDPTGYGRIVRDAEGNVLRIVEQKDASPEELAVREVNTSVYVFRSDVLGRALPALTPANAQKEYYLTDAIALLIKEGAPVRAVRCPDPDEVLGCNDRVQLAQARKLLRQRINERHMLAGVTVLDPASTWVHAEVDLAVDVTLLPGTTLAGATSVAAGATIGPDTTLIDVEVGENATVMRSHVELAVIGAGATVGPFSYLRPGTELGTGGKVGAFVETKKAVIGAGTKVPHLTYCGDAVLGEGVNIGAGTIFANYDGVTKSTTVVGDNSFVGSDSVLVAPVEIAPGSYVAAGSTITTDVGPGELAVARGQGVRIGVRLADPARFDVRGELVCGLDVEIDVNAIFSGRVVLGNGVRIGAHCVIANAEIAAGVVIHPFTHIDGEKLGVRVGAGSLIGPFARLRPGADLGAEVHIGNFVEVKNASLAAGAKANHLAYLGDATVGERVNFGAGSITANYDGANKHRTVIEADVHVGSNCVLVAPVSLGAGGTVGAGSVINKNTPPGSLAVARGKGVTMISPDTIFIDADVTLSPDVTIYPGTHLRGKTKVAEDVVLGPNAIVSDCTIGRGSTIENSTVKESTIGDYTNVGPYAYMRPGSVVGDHVKVGDFVELKNVTIGNHTKISHLSYVGDGEVGDNVNIGCGVVFVNYDGQRKHHTKVEDNAFIGCNANLVAPVSVGRGAYVAAGTTVTREVPADALAVGRVR"
  },
  {
    "reference": "MEVLKKILASLNELHKGQLGGKKRRQPACKHIRKGLEQGHLKKALLNVEWNTCQDCKTTDKESQEKAAEEADEGPSIWLCLKCGHRGCGRNSQEQHALKHYTTPRSDPHCLVLSLDNWSVWCYLCDDEVLYNGSTRLGQLVDYVKKQAHLDTQNTVSKDQENKEFENKKVEKDNRNEEEREKSESLIKGEDLHLNSNTEVSVKGLSNLGNTCFFNAVMQSLSQTPVLRELLKEVKMPGTTFTIEPPEFSIMEPLEIKLDQPGPLTLAMCQFLTEIQETKKGTVTPKELFSQVCKKAVRFKGYQQQDSQELLRYLLDGMRAEEIQRVCGGIFKALSDPDQQNEELEKKIKEYEKKKVIPSFVDRIFGGELTSTIMCEECSTVSGLFPWYNFEKNAKKIKEKEIEDEEEDIDNDSYMKKRDETPGSSKHLQKKAKKLAKKQAKNQRWQQKIQEKVLPLADACDTEQVEENQDSSSESKVEVNSEGSELIQQEESLNSCEEHCSSHKNLNGQENITDVESTGENVGTSEQDGENETGMDVPLEDVASTTESINKFESLSLKDDGHKEHEDVANNLNDLNLDSLTESDEVDIMNGFHASGTKIYKVVNEDPETAFCTLANRDAIKAEESSILHCLYQFTCNERLSENNKLLCDVCTQRQHNGSKTTGKKKHVYTNAKKQMLISLAPPILTLHLKRFQQTGFNLQKVNKHIKFPEVIDLAPFCTTKCKNVAEGNTRVPYSLYGVVEHSGTMRSGHYTAYVKMRPANNHLSDLV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design must be focused on enhancing the efficiency of positive regulation of transcription by RNA polymerase II.2. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.3. For general function, the protein need meet that Does not deubiquitinate histone H2B.; Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis The designed protein sequence is ",
    "response#1": "RKGSLMAPHVLKVHGSSWPAELIELCQMADAKLLKGEIEVPDTWNSGDIYLSSKTIKALQGTIGAIETGVDSIFKGPSAEHISNRAFVAIRPPGHHCHYGTPSGFCLLNNAHVAIEYAYDTYNVTHVVVLDFDLHHGDGTQDICWKRAGFKPEEEPEDSSYDDFGKKFAEFPKVGYFSMHDINSFPTESGFATKENIKNASTCIMNSHDLNIWNIHLSKWTTEEEFNVLYRTKYRTLFAKADEFFRSAKLEMNQQGRPFKGLVVISAGFDASEFEQTSMQRHSVNVPTSFYTTFTKDALKLAQMHCHGKVLSLMEGGYSDKAICSGVFAS",
    "response#2": "GLSNLGNTCFFNAVMQNLSQTPVLRELLKEVKMSGTIVKIEPPDLALTEPLEVNLEPPGPLTLAMSQFLSEMQENKKRVVTPKELFSQVCKKATRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEEVKNKVKDYEKKKAIPSFVDRIFGGELTSTIMCDECRTVSLVHESFLDLSLPVLDDQSGKKSINDKNVKMTMEEEDKDSEEEKDDSYMKSRSDLPSGTSKHLQKKAKKQAKKQAKNQRRQQKIQERFLHFNELCATDYTEDNEREADTALAGEVEVDTDSTHGSQEEATQIELSVNQKDLDGQESMIERTPDVQESPEDLGVKSANTESDLGIVTPAPECPRDFNGAFLEERTSGELDIINGLKNLNLNAAVDPDEINIEIPNDSHSAPKVYEVMNEDPETAFCTLANREAFSTDECSIQHCLYQFTRNEKLQDANKLLCEVCSRRQCNGPKANIKGDRRHVYTNAKKQMLVSLAPPVLTLHLKRFQQAGFNLRKVNKHIKFPEILDLAPFCTLKCKNVAEESTRVLYSLYGVVEHSGTMRSGHYTAYAKR",
    "response#3": "RKGSLMAPHVLKVHGSSWPAELIELCQMADAKLLKGEIEVPDTWNSGDIYLSSKTIKALQGTIGAIETGVDSIFKGPSAEHISNRAFVAIRPPGHHCHYGTPSGFCLLNNAHVAIEYAYDTYNVTHVVVLDFDLHHGDGTQDICWKRAGFKPEEEPEDSSYDDFGKKFAEFPKVGYFSMHDINSFPTESGFATKENIKNASTCIMNSHDLNIWNIHLSKWTTEEEFNVLYRTKYRTLFAKADEFFRSAKLEMNQQGRPFKGLVVISAGFDASEFEQTSMQRHSVNVPTSFYTTFTKDALKLAQMHCHGKVLSLMEGGYSDKAICSGVFATLL"
  },
  {
    "reference": "MATAGKGSKGKGTGVRFTPEGTQGHPQEGTQGHVHFQEQLHDSAVMVTQDKDGHFLVKVGFLKILHKYEITFQLPPVPNLGKDVCPLPVPNPNLRIISVTSLPEGHNVRCEYTAHKEGVLKEELLLAGHSPTHIKVTVQARVMDRHHGTPMLLDGVRCVGAELEYDSEQSDWHGFD",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must have high specificity towards protein phosphatase 1 binding. The designed protein sequence is ",
    "response#1": "DQLSAAPGVKGMVMTVFTLPSFPYVFKVIKDRLGGTKEFGRQTVIDRYRMVKRHDRVGRMADTLEFVDVALPLKRISADLLDEFKQTIANSISIEGDTLVIHQLFVERRMTPLNLYLEYANDEEVDAAMDDYGRALKEMMAANIFPGDMLLKNFGVTRHKRVVFYDYDEVRYLTDMSFRRLPENDWEVSYAPDDVFPQQLAQFAVPQAKYRNKLLKRHPELIDSAYWCRVQKNIRKGELTDVFPYV",
    "response#2": "DQLSAAPGVKGMVMTVFTLPSFPYVFKVIKDRLGGTKEFGRQTVIDRYRMVKRHDRVGRMADTLEFVDVALPLKRISADLLDEFKQTIANSISIEGDTLVIHQLFVERRMTPLNLYLEYANDEEVDAAMDDYGRALKEMMAANIFPGDMLLKNFGVTRHKRVVFYDYDEVRYLTDMSFRRLPENDWEVSYAPDDVFPQQLAQFAVPQAKYRNKLLKRHPELIDSAYWCRVQKNIRKGELTDVFPYV",
    "response#3": "DQLSAAPGVKGMVMTVFTLPSFPYVFKVIKDRLGGTKEFGRQTVIDRYRMVKRHDRVGRMADTLEFVDVALPLKRISADLLDEFKQTIANSISIEGDTLVIHQLFVERRMTPLNLYLEYANDEEVDAAMDDYGRALKEMMAANIFPGDMLLKNFGVTRHKRVVFYDYDEVRYLTDMSFRRLPENDWEVSYAPDDVFPQQLAQFAVPQAKYRNKLLKRHPELIDSAYWCRVQKNIRKGELTDVFPYL"
  },
  {
    "reference": "MNKNICLSASHKQNNRDDPIINELINYFGNETFTIQSTRIGVPVVWVTREQILDFLIFLKKKPNSYLMLYDLHGVDERLRNNREGLPAVDFTVFYHLLSIKSNSDIILKVPLLEGDISLPTATNIFSNANWYERETWDMFGIIFHGHPHLTRIIMPPSWVGHPLRKDYPARATEFNPFVLTKEKEELEMDSLTFKPEKWGIKHSNHKDDFMFLNFGPNHPSAHGAFRIVLQLDGEEIVDCIPDIGYHHRGVEKMSERQSWHSYIPYTDRVEYLGGCVNEMPYVLAVEKLAGIVVPDRVNVIRIMLSELFRINSHLLYISTYIQDVGGMTPIFLAFTDRQKIYNVVEAITGARMHPAWFRIGGVAHDLPHGWNMLLKELLDWLPNRLEHYVKVALENSILRSRSEGIASYSAKEALEWGVTGAALRATGINFDVRKWRPYSGYDNFEFEIPVGNGISDCYSRVMLKVEEIRQSIRILKQCLHNMPAGPFKADHPLTTPPPKERTLQHIETLISHFLQVSWGPIIPANESFQMIEATKGINSYYLTSDGNTMSYRTRIRTPSFPHLQQIPSVIRGSLISDLIVYLGSIDFVMSDVDR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must have high specificity towards NAD binding. The designed protein sequence is ",
    "response#1": "IGAGPGDPDLMTFKALRLLQRADVVLYDRLVAPEIVELCRKDAERIFVGKARSNHAVPQQDINALLVEHAKQGRKVARLKGGDPFIFGRGGEEIEQLANESIPFQVVPGVTAAAGCASYAGIPLTHRDYAQSVRFVTGHLKDNSINLPWAELVHGHQTIVFYMGLVGLPVICEQLIAHGRSADTPIALVQQGTTRHQKVVVATLATMVEEVAKNPVKAPTLIIVGEVTG",
    "response#2": "IGAGPGDPDLMTFKALRLLQRADVVLYDRLVAPEIVELCRKDAERIFVGKARSNHAVPQQDINALLVEHAKQGRKVARLKGGDPFIFGRGGEEIEQLANESIPFQVVPGVTAAAGCASYAGIPLTHRDYAQSVRFVTGHLKDNSINLPWAELVHGHQTIVFYMGLVGLPVICEQLIAHGRSADTPIALVQQGTTRHQKVVVATLATMVEEVAKNPVKAPTLIIVGEVAA",
    "response#3": "GEVYLIGAGPGDPDLMTFKALRLLQRADVVLYDRLVAPEIVELCRKDAERIFVGKARSNHAVPQQDINALLVEHAKQGRKVARLKGGDPFIFGRGGEEIEQLANESIPFQVVPGVTAAAGCASYAGIPLTHRDYAQSVRFVTGHLKDNSINLPWAELVHGHQTIVFYMGLVGLPVICEQLIAHGRSADTPIALVQQGTTRHQKVVVATLATMVEEVAKNPVKAPTLIIVGEVVSLARD"
  },
  {
    "reference": "MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTPPGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGPTLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKITPVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFVHAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHTFRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEYELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFHPGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHRMGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHPRLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIELTDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALKAKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDANYYLMEAYKHLKPIALAGDARKFKATIKIADQGEEGIVEADSADGSFMDELLTLMAAHRVWSRIPKIDKIPA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide..2. The designed protein must have a active site that is structurally stable and resistant to denaturation.3. The protein design should prioritize the formation of helix-strand-turn-helix-helix-helix-strand-strand-helix-strand-strand-turn-helix-strand-strand elements.4. The heme binding site should be stable and able to withstand changes in environmental conditions.5. A protein that contributes to response to oxidative stress by functioning in cytoplasm with iron ion binding. The designed protein sequence is ",
    "response#1": "GFTVGILASTSSSTSISQAAQLATSFSSRGIRAVIVGESLLSGTDQTYSSADATAFDAVVVTMGAETLFGPVAKPNTLFPSGRPSQILHDAYRWGKPVGAVSKASVVLEPLPGTKNQGGVYRVESVNELATSIAKGLETFRFVDRFPLV",
    "response#2": "GVELVHAANKGLLKALFLVIPIKSKYAGVTYADIFQLASATAIEEAGGPKIPMIYGRADVADGEECPPEGRLPAADPPSPAEHLREVFYRMGLSDKEIVALSGAHTLGRARPERSGWGKPETKYTENGPGAPGGQSWTSEWLKFDNSYFKEIKERRDEDLLVLPTDAVLFEDSSFKIHAEKYAEDQDAFFEDYAEAHAKLSNLGAKFDPPKGISLL",
    "response#3": "GVELVHAANKGLLKALFLVIPIKSKYAGVTYADIFQLASATAIEEAGGPKIPMIYGRADVADGEECPPEGRLPAADPPSPAEHLREVFYRMGLSDKEIVALSGAHTLGRARPERSGWGKPETKYTENGPGAPGGQSWTSEWLKFDNSYFKEIKERRDEDLLVLPTDAVLFEDSSFKIHAEKYAEDQDAFFEDYAEAHAKLSNLGAKFDPPKGISLC"
  },
  {
    "reference": "MPKFIFVTGGVVSSVGKGITTASLGRILKSRGIKVSIQKLDPYLNVDPGTMSPYQHGEVFVTVDGAETDLDLGHYERFIDQDLTAASSVTSGQIYLAVLERERRGDYLGGTIQQVPHLTNEIKARIYGVAEQTGCDVLICEVGGTVGDIEGETFIEAIRQIRHEQPRDATLSVHVCFLPWVGATGELKTKPTQHSVRELRSKGIQPDAIVLRSDHPVPRDITDKVALFCDVEPRAVIPMETADTIYEVPITLEERGLGDFVLDRLGLEGRRDLAEWRDLVYRLKHPRRTAEVAVVGKYVELRDAYISVKEALVHAGIAHEADVTIRWVPAEALETRDPADLLAGIDGIVVPGGFGERGWEGKIRAAEYARTTGTPYLGLCLGMQALVTEFARHACGLVGANSTEFDPETPHPVISLLEEQHAVVNLGGTMRLGAYPCRLLPGTLAHRAYGTDVVSERHRHRWEFNNAYRARLEAAGLRVSGTSPDGALVEISEISGHPFMLGTQFHPELQSRPNRPHPLFREFVAAALARRAAGAAAPATA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with ATP binding functions is required to enable glutamine metabolic process.2. A protein that can modulate the overall flux of metabolites through the CTP from UDP: step 2/2 pathway, enhancing or suppressing its activity as needed.3. The designed protein should have a high specificity for L-glutamine, ATP binding, with minimal off-target effects.4. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. The designed protein sequence is ",
    "response#1": "LPDAYLSVAEALRAGGFDNDARVNLRWIASDECATPEAAARLLGDVDAVCIPGGFGVRGIEGKIGAIRYAREQGIPILGLCLGLQCMVIEVARDLAGLVEANSSEFDPDAEQPVIATMEEQKHIVSGSGDLGGTMRLGLYPANLADGSIVRDLYGAPSVQERHRHRYEVNNAYRDKLETAGLVFSGLSPDNELVEFIELDRSQHPYFVATQAHPELRSRPTKPHPLFSGLR",
    "response#2": "EDAYKSIAEALTHGGMANRVKVRIEWVDAELFDKEDATPYLQGFHAILVPGGFGERGTEGKIKAAQYAREHKVPYLGICLGMQMAVIEAARNVAGIEEAGSEEFDHEAGKKRFEPVVYHLKEWVQGNHKVSRSADDDKGGTMRLGAYDAALTEGSKVAEVYGSNAIEERHRHRYEVDIKYREQLEACGLKFTGMSPDGRLPEIVEWTDHPWFIGVQFHPELKSKPFDPHPLFKDFVDTAYQL",
    "response#3": "ITVAIVGKYVALHDAYLSVVEALDHAGIATGTKVNIRWIDSEELDDTSVDPKEVFEGVEGIIVPGGFGSRGVEGKIRTIQYARENNIPYLGLCLGMQSAVMEFARNVCGMEGATSSEFDENAKYKVIDLMSDQVDVDKKGGTMRLGIYPCKVEAGTKTHEAYGEDLIYERHRHRYEFNNEYRQQLTDAGLVISGTSPDGRLVESIEVKNHPFFIGTQAHPELKSRPNNAHPLFRGFIQK"
  },
  {
    "reference": "MPDFKNVSINKSANIFYEGRVTSRSLEFEDGSRKTLGIMLPGEYQFNTVHKEIMEINSGELEYRLPAEDWKKITAPGVFEVPANSKFDIKIIAIVDYCCSFIK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be able to perform pyrimidine-nucleoside phosphorylase activity, purine-nucleoside phosphorylase activity, thymidine phosphorylase activity under specific conditions. The designed protein sequence is ",
    "response#1": "MNFL",
    "response#2": "MKLFKI",
    "response#3": "MPIGVPKVPFRSPGESDAAWFDLY"
  },
  {
    "reference": "MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTDKAAPQLLVATDKPTTKGEPLISDIVSDAQQANLLIPVDETPPVINDEQSTSTPLTTAQTMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKPTLFTKLTSEQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPHQTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLKVDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQQQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Controls the length of the flagellar hook..2. The protein should have a specific function in bacterial-type flagellum-dependent cell motility, bacterial-type flagellum assembly and should be localized to bacterial-type flagellum hook in order to carry out this function efficiently.3. The protein should exhibit Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "FRVMGTKPVAFLIAGFAKEREPFSVHFGAGGEMFLTTPLAVEPEKGLLIFDCSGSVESNQRFLRSDRNIFIGRPGGVHVQFTTGAAREVAHEGGKAFAVALPQYIVG",
    "response#2": "FRVMGTKPVAFLIAGFAKEREPFSVHFGAGGEMFLTTPLAVEPEKGLLIFDCSGSVESNQRFLRSDRNIFIGRPGGVHVQFTTGAAREVAHEGGKAFAVALPQYIVK",
    "response#3": "FRVMGTKPVAFLIAGFAKEREPFSVHFGAGGEMFLTTPLAVEPEKGLLIFDCSGSVESNQRFLRSDRNIFIGRPGGVHVQFTTGAAREVAHEGGKAFAVALPQYIVG"
  },
  {
    "reference": "MPRRREVPKREILPDPKFGNVELSKFMNVIMEGGKKAVAERIIYGALELIQKKHPDKDPLEAFTVAINNVKPMVEVKSRRVGGANYQVPVEVRPVRRLALSMRWLKEAARKRGEKSMAQRLANELLEATEGRGGAMKRRDEVHRMAEANKAFSHFRF",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit.2. A protein that can perform rRNA binding, structural constituent of ribosome in small ribosomal subunit to support translation. The designed protein sequence is ",
    "response#1": "KKYISLMPVYLPHTGGRHEHRRFGKAKVPIVERLINQIMRPGRNKGKKHLAYNIVKLAFDIIYLKTGQNPIQVLVRAIENSAPREEVTRIMYGGIVYYVAVDVSPQRRIDLALRHIATGAKDASFNNPKPIEEVLAEEIIAAANNDSKSFAIKRKEEIERIALSSR",
    "response#2": "KKYISLMPVYLPHTGGRHEHRRFGKAKVPIVERLINQIMRPGRNKGKKHLAYNIVKLAFDIIYLKTGQNPIQVLVRAIENSAPREEVTRIMYGGIVYYVAVDVSPQRRIDLALRHIATGAKDASFNNPKPIEEVLAEEIIAAANNDSKSFAIKRKEEIERIALSSV",
    "response#3": "KKYISLMPVYLPHTGGRHEHRRFGKAKVPIVERLINQIMRPGRNKGKKHLAYNIVKLAFDIIYLKTGQNPIQVLVRAIENSAPREEVTRIMYGGIVYYVAVDVSPQRRIDLALRHIATGAKDASFNNPKPIEEVLAEEIIAAANNDSKSFAIKRKEEIERIALSSI"
  },
  {
    "reference": "MYELNNFRSIKIALASPEKIRQWSKGEVKKPETINYRTLKPEKEGLFCEKIFGPTRDWECHCGKYKRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIPSRMGLLLDMSPRSLEKVLYFAAYIVINPGETPLTEKQILTEKEYADAIEKYGPKFKAGMGAEAVKGLLESIDLDAMNIELRHKLKESTGQKRIRTVRRLEVVEAFRKSKNNPAWMILDVVPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPDIIVRNEKRMLQEAVDALIDNGRRGKPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKFYQCGLPKQMALELFKPFVMKKLVQENYAHNIKSAKRMVEKVKPEVWGVLEEVISQHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADFDGDQMAVHVPLSVEAQAESRFLMLSINNILAPKDGEPITTPTQDMVLGCYYLTIQDEGVLGEGTIFKDFEELLLAYETKAVSLHAKVKVRMKLSAEDSGKLVESTVGRFIFNEKIPQDLGFVDRSKDLYALEVDFLCTKGALGKVIGKCFRKHGNTITSIMLDYIKELGFKYSTRGAITVAVSDMDVPEAKAELISQAEQRVDKYEKAFRRGLISDEERYEKVIETWTETTEKVTDALMAGLDRFNNIFIMADSGARGSKNQIRQLGGMRGLMANASGHTVEIPIKSNFREGLTVLEYFTSTHGARKGLADTALRTAD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have zinc ion binding, magnesium ion binding, DNA-directed 5'-3' RNA polymerase activity and be active in DNA-directed RNA polymerase complex.2. The protein should have a low Km value for a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +   RNA(n+1).3. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "DWMIIDVIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKKLLDLGAPDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKMYQCGLPKEMALELFKPFVMKKLVQGGLSHNIKSAKRMVERVQSEVWDVLEEVISDHPVLLNRAPTLHRLGIQAFQPVLVEGRAIKLHPLVCTAYNADFDGDQMAVHLPLSVEAQAEARFLMLAAHNILKPSDGKPVCVPTQDMVLGSYYLL",
    "response#2": "GKRVDYSARTVISPDPNLDINEVGVPVHIATRLTVPDIVTERNIDEMRQLIKNGPSKHPGALYIIRPDGKRIRLEFVADKKLVAESIEPGFVIERHLKEGDIALFNRQPSLHRMSIMAHKVKVFPYRTFRMHLTVCPPYNADFDGDEMNLHIPQSKEAQTEARLLMQL",
    "response#3": "AWMILTILPVIPPALRPMIQLEGGRFATSDLNELYRRIITRNNRLLRLLEIDAPQLIIRNEKRLLQEAVDTLIDNGKRGKIALSANNRPLKSLSDIIKGKHGRFRQNLLGKRVDYSGRSVIVVGPSLRLNQCGLPYEMAIELFQPFIIRELINQGLASNMKIAKNLIQQNESIIDPVLEKVLKSHPIFLNRAPTLHRLGIQAFEPILVQGRAIKLHPLVCSAFNADFDGDQMAVHIPLSLEAQAECYMLMLAPYNFLSPANGEPIIMPSQDMVLGCYYLI"
  },
  {
    "reference": "MILSEILKQSDYKIIKGTIEKEIKAIQHHSDKIEKDTLFVAISGYQRDGHDFIEQVIRDGATAVVVERHIDLDQIPEAVSVIRVVDTKLALAEMVNAFYDHPTEKINVVGITGTNGKTSITQMLNNLYTSFGQKNAVFGTIANFIGDEKIPTVNTTPDALELARLMNRASEVACDNCFMEVSSHALKMGRVHGMHFNYAVFTNLTEDHLDFHPDFEDYFMAKLQLFKMALKGCVINNDNVYGRRILKEVSLDKRCLSYGLTKASELWANHIEYHQWGSTFNLNWGEQHIPVRLNIPGKIYVYNVMACVGVMILQGFKMDAIGAFVSEIEPVRGRLESVSNDHSRTILLDYAHTPDALENVLHAIREFSRGKIITVFGCGGDRDRKKRPMMGAIAQKLSDLVILTSDNPRTEAPNQIIKDVLEGMTVSDQLYVVEDRREGIYKALSLASENDVVLIAGKGHEDYQIIGKTKIHFDDKEVVQDFYDGRK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding site should be located in a region of the protein that is accessible to the ligand.2. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.3. The designed protein have Mur ligase N-terminal catalytic domains with specific sequence motifs that are crucial for its activity.4. The protein should be optimized for activity in peptidoglycan biosynthesis, Cell wall biogenesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.6. The protein design should aim to mimic or enhance the function of natural proteins involved in peptidoglycan biosynthetic process, cell wall organization, cell division, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "MKLRQLISQLPDLKIEGEAVTGFAIDHRKVAPGTVFGAFRGAKFNGEDFIAAAIEAGAVAVVAAPDARVSDVPQIRVDDTRYALALAAANFYRHPAKQLKLTGITGTKGKTTTTYLIRSMLENAGHKVGLIGTIEVIIGDEHIHAENTTPESYNLQEYMAKMVEAGCDAVVMEVSSQGLMLHRSQGFTFDIGIFTNIEADHIGPNEHKDFDDYMHCKGLLFKQCRIGICNGDDIHTDDILEGHTCEVETYGFNKDVDIRAVNLAYIRKPGNLGVFFDTEGLIDMHAEIVTPGKFSVYNALCAIAVAGLAGIKPSDIETGLSQVTVVPGRMEQVQIRSDQDISVIVDYAHTPDSLENMLRAARPFISGRMICVFGCGGDRDRTKRPKMGAIAAELSDWPVVTSDNPRTEDPERILQDILTGIPASVNPTVIADRAEAIRTAILQAQPGDGVLIAGKGHETYQLIGDKRVHFDDREVARQALQCRE",
    "response#2": "MKITGLSVDSRMVKPGHLFAALPGSQVHGADFVGHALRLGAAAILTDRKGARQAAGALAQSGAALVVTEDPRQALAGAAALCAAHFFGHPAHHLAAIGITGTNGKTTSTYILEKIIADQGKPSALIGTIQYRYRDKVHPIAHTTPEALELQDLLAAMRASGASYAAMEVSSHALAQGRVDGCRFAVGAFTNLTQDHLDFHPTMDDYFAAKGLLFSDYLKNDAFAVVNMDDTWGERIAKATRVPVITYGIDNRADFRATDIRMTSTGTSLNLVTETGHVDVSLKMIGRFNVYNTLAAIGAGIAAGVPIDVVVQGLKGVRGVP",
    "response#3": "MKLKDILYKVAIEAVTGSTEIAEVTGVSLDSRSVLPGDLYAALPGAVTHGAAFVAKAEQSGAVAVLTDPAGEERARETGLPVLVVDRPRKVLGAVAARVYDNPSQKLKLVGVTGTNGKTTTTHLIEFLLLQSQKKSAILGTLGYGMPGALHATQRTTPDAVEIQKVLAELKQQNVEYVVMEVSSHALAQHRVAGIQFESAVFTNLSQDHLDYHKTMEEYGAAKKLLFAGLDFAKVGVVNTDDAYGRRLSEQLTQDVITYSVENEQADLYATNIKTTVTGVEFDLHTPWGSGHITSKLIGKFNVYNVLAAVTAGLALGVSLTAVVQGIESAKGVPGRIEMVPVSDQFTLLIDYAHNAMSLKSLLTTLREYQPKRLVCVFGCGGNRSRIRRFEMGEVSGNYADFTIITSDNPRFEKPQDIIEDIKTGINKTSGRYMEICDRKEAIAYAIGHGQPGDIIVLAGKGHEDYQEIKGVKYPMDERVLIADILKELN"
  },
  {
    "reference": "MGGKWSKSSVVGWPQVRERLRREETRRERARQTPAIGVGAASQDLARHGAITSENTAGTNADVAWLEAQEEEEVGFPVRPQVPLRPMTYKGAHDLSFFLKEKGGLEGLIYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGTRFPLTFGWCFKLVPMDPEEVEKANEGENNSLLHPICQHGMEDDDREVLVWRFDSSLAFRHLARELHPEFYKDC",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should emphasize Basic and acidic residues compositional bias.2. The designed protein should be involved in suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I by localizing to membrane, virion component, host cell plasma membrane, host cell Golgi membrane, extracellular region and exhibiting GTP binding, SH3 domain binding.3. For general function, the protein need meet that Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike4. Incorporating a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Dileucine internalization motif; necessary for CD4 internalization, Diacidic; necessary for CD4 internalization motif is critical for the successful design of the protein. The designed protein sequence is ",
    "response#1": "MGGKWSKRSVPGWSAIRERMRRAEPAAEGVGAVSRDLERHGAITSSNTAATNADCAWLEAQEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLVYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRYPLTFGWCFKLVPVEPEQVEKANEGENNCLLHPMSLHGMDDPEGEVLQWKFDSRLAFHHVAREKHPEYYKDC",
    "response#2": "MGNKWSKSSIVGWPDIRERIRRTPPAAEGVGAVSQDLDKHGAITSSNVNHPSCVWLEAQEEEEVGFPVRPQVPLRPMTYKAAFDLSFFLKEKGGLDGLIYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGTRYPLTFGWCFKLVPVDPKEVEEANKGENNCLLHPMSQHGMEDEEGETLKWRFDSSLARRHLARELHPEFYKDC",
    "response#3": "MGGKWSKSSIVGWPEIRERMRRAEPAAEGVGAASRDLDKYGAITSSNTPANNAACAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLDGLIYSQKRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPDKVEEATEGENNCLLHPISQHGMDDADREVLVWKFDSRLAFHHMARELHPEYYKNC"
  },
  {
    "reference": "MELSQTALFIGSIINLNALVLILRAWLQFARVDYYNPVSTFAVKMTDPVLKPLRKIAPTVKNIDTSALLLIFIIGMLKGIIYFGLSVNVLLVLGVLTVLKSIGLAIFYVLFIGAVLSWFNRGNNSISYAFYQLSEPLLKPIRRLLPTLGMIDFSPMVVMFILLFLNNFMLDLLGGLWIIAG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a specific function within the plasma membrane.2. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "GRTANGKVRLYVIGQGALSAAVAANSKHDIAEAERWNLPIILIVLLAVFGSLAAAAVPLALGVCTVVVTMGLVDLVSMHTIMSVFVTSTVSMFGIALAVDYSLFILMRFREELRSGRQPQEAVDAAMATSGLAVVLSGMTVIASLTGIYLINTAALKSMATGAILAVAIAMLASITLTPAALATFGRAAVKRSVLMHWSQRSECTQSLFWTRWVGWVMHRR",
    "response#2": "GRTANGKVRLYVIGQGALSAAVAANSKHDIAEAERWNLPIILIVLLAVFGSLAAAAVPLALGVCTVVVTMGLVDLVSMHTIMSVFVTSTVSMFGIALAVDYSLFILMRFREELRSGRQPQEAVDAAMATSGLAVVLSGMTVIASLTGIYLINTAALKSMATGAILAVAIAMLASITLTPAALATFGRAAVKRSVLMHWSQRSECTQSLFWTRWVGWVMHRR",
    "response#3": "GRTANGKVRLYVIGQGALSAAVAANSKHDIAEAERWNLPIILIVLLAVFGSLAAAAVPLALGVCTVVVTMGLVDLVSMHTIMSVFVTSTVSMFGIALAVDYSLFILMRFREELRSGRQPQEAVDAAMATSGLAVVLSGMTVIASLTGIYLINTAALKSMATGAILAVAIAMLASITLTPAALATFGRAAVKRSVLMHWSQRSECTQSLFWTRWVGWVMHRR"
  },
  {
    "reference": "MPHATLRQAVSIARASLRSRPGRLPSLPRRFLSSPPQNSRDDKPENHSSGNNHHHDNGNRPRQTANIKFLPFIAIIAVGSAAYVALVNRRKAIAMPPAADSQQDLPPPSSTPTFSPEKATVVFVLGGPGAGKGTQCARLVDQYAFTHLSAGDLLRAEQDRAGSQFGDLIRSHIKDGLIVPMEVTVQLLENAMTEAMARPGPRPARFLIDGFPRKLDQAYRFEGSVCEPSLVLFYDCPENVLRDRLLERGKTSGRADDNAESILKRFRTFVETSMPVVDHYDKRGAVIKIDATRDKDAVFADTQKRLKEKLGDDF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "LHHHHHH",
    "response#2": "MP",
    "response#3": "MG"
  },
  {
    "reference": "MFKDNKKLKYILIYGSMVLGMIFMVNYTRTIMTTKEIPYNEFNQMLKDDQVKAVQIARDKITIEPKKDTKYAGKTLYTVNVGDPDLIKDLNTANVTYNGIISNGVSVLDIALSWILPLLIFFLIGKLLFGRMDKKMGGGVMSFGKNTAKLYAEDETGKTFKDVAGQEEAKESLVEIVDFLHDPNKYTEIGAKLPKGALLVGPPGTGKTLLAKAVAGEAKVPFFSMSGSDFVEMFVGMGAARVRDLFRQAEEKAPCIVFIDEIDAIGKSRDGAIQGNDEREQTLNQLLSEMDGFDSSKGVVILAATNRPEILDKALLRPGRFDRRVIVDRPDLQGREAILKVHAKDVRLADNVDFLSIAKSTPGAVGADLANIVNEAALRAVKHNRKTVIQEDLEEAVEVIIAGKEKKDRILSEKEKKIVAFHEVGHALVAALLKNTDPVHKITIVPRTMGALGYTMQQPEEDKYLVTKEEMLSEISTLLGGRAAEEVEFNTISTGASNDIERATQSARSMVTIYGMTDKFDMMALESVTNRYLDGRPVQNCSAKTAAIIDQEALAIIKECHRKSIDILKNNRDLLSKISEALLEKETLLGNEFMEFIYEKYPEMKKEVEEKEKKKAEIEAKKKEILAERIAEREELQRKAQEIKKLEDQKKVLEQIHEETVDLDGNNSSVNDEIKNYLESSEDHTLNEEHKKVDTDEEK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Incorporate a signal peptide in the protein design.2. The protein must exhibit ATP hydrolysis activity, ATP binding, zinc ion binding. The designed protein sequence is ",
    "response#1": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#2": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#3": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR"
  },
  {
    "reference": "MKMIPGGVTAPRGFQASGVHCGVKKGKGDGNQPPKSGRPEVLDQKKDLALIVSEKECAAAAMYTLNRVKAAPLYVTMDHLENGVAQGIIANSGNANACCPMSHENAEEMCALAAAATGLKAADFVVASTGVIGQTLNIAAIARGVPAAAAALSRSGSGDAANAIMTTDTVKKELAVTVAIGGQKVTIGAIAKGSGMIHPNMGTMLCFLTTDCAITQEMLQDALHDIVPRTFNRVTVDGDTSTNDMCVILANGMAENPLIEWKDDNYTVFFKALSEICTELARSIAADGEGASRLVTCTVMDARSEESAERLAKAVVGSSLVKAAMFGADANWGRVLCAMGYSKAPFRPEFVDVTFRSVVGEVTVCRQGAGLDFDEDQAKSILSQDEVVIEVDLHEGEHQATCWGCDLTYEYVKINGDYRT",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The substrate binding site should be located in a region of the protein that is accessible to the ligand.2. The designed protein must be optimized for methione N-acyltransferase activity, acetyl-CoA:L-glutamate N-acetyltransferase activity.3. The protein should be optimized for activity in Amino-acid biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile to enable effective catalysis of acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate. The designed protein sequence is ",
    "response#1": "QFVDWLRAVAPYIHAFRDKTFVIGFGGELVKAGMLGALVNDIALLHAMGMHIVLVHGSRPQVEEQLALRHVQTQFVDGIRVTDNAALESAKEASGELRLDIEATFSQALPNTPMAGARISVVSGNFVTARPVGIVNGVDFQHTGLVRKIDAESIQHSLSNRKIVLLSPLGFSPTGQAFNLSMEDVATNTATALKADKLIFITEVPGIMDRVGKLQQELSMESAIERLREGRLSADTAYYLQHIVKAMRGGVRRAHLIPFALDGSILLELFLHDGVGTMDIANFSVIEHDGIIFGCAALYPYPKEGVGEMACLTVAPDTQGTGDGERLLKHVEARARAVGLKRLFVLTTRTEHWFLKRGE",
    "response#2": "KTFVIGFGGELVKAGMLGALVNDIALLHAMGMHIVLVHGSRPQVEEQLALRHVQTQFVDGIRVTDNAALESAKEASGELRLDIEATFSQALPNTPMAGARISVVSGNFVTARPVGIVNGVDFQHTGLVRKIDAESIQHSLSNRKIVLLSPLGFSPTGQAFNLSMEDVATNTATALKADKLIFITEVPGIMDRVGKLQQELSMESAIERLREGRLSADTAYYLQHIVKAMRGGVRRAHLGA",
    "response#3": "QFVDWLRAVAPYIHAFRDKTFVIGFGGELVKAGMLGALVNDIALLHAMGMHIVLVHGSRPQVEEQLALRHVQTQFVDGIRVTDNAALESAKEASGELRLDIEATFSQALPNTPMAGARISVVSGNFVTARPVGIVNGVDFQHTGLVRKIDAESIQHSLSNRKIVLLSPLGFSPTGQAFNLSMEDVATNTATALKADKLIFITEVPGIMDRVGKLQQELSMESAIERLREGRLSADTAYYLQHIVKAMRGGVRRAHLIPFALDGSILLELFLHDGVGTMG"
  },
  {
    "reference": "MEIKQMLVPVSRYSVLCPYEMNPTEITFHNTYNDAPAINERNNVANNSTGTSFHIAVDDKEAIQLIPFNRNAWHAGDGTNGRGNRHSIGVEICYSQSGGARYRKAELNAVEVIAQLMIQFDIPISKVKTHQERNGKYCPHRMLDEGRVQWFKNQCANRASSIKNSNKTQETGKVEIIVNKFNKVVTYEFGTALVPEMLGMMDALGYESRIISYGDKQGLVRFETAYRQGNELDKATAWLDAKGLKYFYTKE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. The designed protein sequence is ",
    "response#1": "EESARFTNGNQPITVRKVGPFLSCPVGYQFQPGGYCDYTEVMLQDGHVWVGYTWEGQRYYLPIRTWNGC",
    "response#2": "YYMEESARFTNGNQPITVRKVGPFLSCPVGYQFQPGGYCDYTEVMLQDGHVWVGYTWEGQRYYLPC",
    "response#3": "YYMEESARFTNGNQPITVRKVGPFLSCPVGYQFQPGGYCDYTEVMLQDGHVWVGYTWEGQRYYLPC"
  },
  {
    "reference": "MPAEFSSVTVVVPANIYFDGKVTSRTVRFSDGSEKTLGLMLPGDYEFGTSKPELMEVTAGGAEVLLPGESEWRTYGPGDSFNVPGDAKFQLRVSEPFDYVCTYLNE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein have enhanced phosphate + xanthosine = alpha-D-ribose 1-phosphate +   xanthine.2. The protein must exhibit the following characteristics: Also catalyzes the reverse reactions.; Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates; Catalyzes the phosphorolysis of diverse nucleosides, yielding D-ribose 1-phosphate and the respective free bases.3. The protein must exhibit uridine phosphorylase activity, thymidine phosphorylase activity. The designed protein sequence is ",
    "response#1": "MXXXRXG",
    "response#2": "LFKW",
    "response#3": "MEKRLIRNT"
  },
  {
    "reference": "MLESKLINHIATQFLDGEKDGLDSQTPLFELNIVDSAAIFDLVDFLRQESKVSIGMQEIHPANFATVQSMVALVQRLKAHPEQGGAA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Involved in the biosynthesis of 4-methoxy-2,2'-bipyrrole-5- carbaldehyde (MBC), one of the terminal products involved in the biosynthesis of the red antibiotic prodigiosin (Pig); Carrier of the L- prolyl group transferred from L-prolyl-AMP by PigI.2. The protein design should prioritize the formation of helix-strand-turn-helix-turn-turn-strand-helix elements.3. A protein that can modulate the overall flux of metabolites through the Antibiotic biosynthesis, prodigiosin biosynthesis pathway, enhancing or suppressing its activity as needed.4. The designed protein must have at least one Carrier domain.5. The protein design should be able to enhance the efficiency of antibiotic biosynthetic process. The designed protein sequence is ",
    "response#1": "VEPLAVIGMSCRFAPDLDTPGRLWEFLRAGGSAVGEMPDRRWDPYVTDSRTRDILRTTTRKGSFMRDIEGFDAEFFQITPREAEYIDPQQRIMLELAWEALCDAGLPPTSLAGTDASVYVAANSNDYGRRLLEDLDRTGAWAVNGTTFYGIANRISYFLDAHGPSMAVDTACAGSLTALHVAGQALHRGETSVAIVGGINIMASPALVVALDAASATSPDGRSKSFDKAADGYGRGEGGGVVVLKRLSDAVRDGDPVHGLVLASGVFQDGRSDGMMAPNGSAQQRMLEEIYRRSGIDPGTVQYVEAHGTGTQLGDAAEAQAIGNVFGPGRDGDNPLLIGTLKPNVGHVEAASGIAGVIKVLLGMRHGELPPSPHEEPDPGLGLEARGLRLVAEPTPWPRGEHGMRAGVSSYGVGGSIAHAVLQDAPKPEQDTDGPTLNQWKSTADRILNLDEIYEQCRSQELVHTGMMKAEGQIYEAKEGAVIDLAVGQEALRHSDAFLFHPTLIDGSGIGSSCLISDQTMYLPLYYESFSASERLQKGCTARILSSSVRQKKELTYMTIEYFNSAGQKVAELKQFAGKSVRVFSGHGAQWSGMGRELLTTEPVFAQVIDELAPVFSEELGWTPREAIEAGGPWTVVRTQAMTFAMQVALAEVWSDLGLRPGAIIGHSVGEIAAAAVAGSLDRAEAARFACRRARALGKIAGRGAMAMVPMAFADVEQRVAGRDAVVAAIAASPLSTVVSGDTAAVEALLADLEADGIQARRVNTDVAFHSPHVQEILDEVRQAAAALRAGTPRVTLYSTALA",
    "response#2": "VEPLAVIGMSCRFAPDLDTPGRLWEFLRAGGSAVGEMPDRRWDPYVTDSRTRDILRTTTRKGSFMRDIEGFDAEFFQITPREAEYIDPQQRIMLELAWEALCDAGLPPTSLAGTDASVYVAANSNDYGRRLLEDLDRTGAWAVNGTTFYGIANRISYFLDAHGPSMAVDTACAGSLTALHVAGQALHRGETSVAIVGGINIMASPALVVALDAASATSPDGRSKSFDKAADGYGRGEGGGVVVLKRLSDAVRDGDPVHGLVLASGVFQDGRSDGMMAPNGSAQQRMLEEIYRRSGIDPGTVQYVEAHGTGTQLGDAAEAQAIGNVFGPGRDGDNPLLIGTLKPNVGHVEAASGIAGVIKVLLGMRHGELPPSPHEEPDPGLGLEARGLRLVAEPTPWPRGEHGMRAGVSSYGVGGSIAHAVLQEYAGQHTENHHPGSGPVLLLTSGSTGTPL",
    "response#3": "VEPLAVIGMSCRFAPDLDTPGRLWEFLRAGGSAVGEMPDRRWDPYVTDSRTRDILRTTTRKGSFMRDIEGFDAEFFQITPREAEYIDPQQRIMLELAWEALCDAGLPPTSLAGTDASVYVAANSNDYGRRLLEDLDRTGAWAVNGTTFYGIANRISYFLDAHGPSMAVDTACAGSLTALHVAGQALHRGETSVAIVGGINIMASPALVVALDAASATSPDGRSKSFDKAADGYGRGEGGGVVVLKRLSDAVRDGDPVHGLVLASGVFQDGRSDGMMAPNGSAQQRMLEEIYRRSGIDPGTVQYVEAHGTGTQLGDAAEAQAIGNVFGPGRDGDNPLLIGTLKPNVGHVEAASGIAGVIKVLLGMRHGELPPSPHEEPDPGLGLEARGLRLVAEPTPWPRGEHGMRAGVSSYGVGGSIAHAVLQEAPDAAAVPRVAVTGIGVVAPGGIGVPAFWDLLSSGRTATRGITLFDPEGLRSRIAAECDFDPLAHGLDPELVERADRYIQFALVAADEAVTDSGIDFGTENPWRVAVSLGSAVGGTTRLEHDYVLVSERGQRWDVDHRAAEPELHRAFSPSTLAADVAERFGAQGPVQTVSTGCTSGLDAVGYAFHTIEEGRADVCIAGASDSPISPITMACFDAIKATSPNNDDPEHASRPFDAHRDGFVMGEGAAVLVLEELEHARARGAHVYCEIGGYATFGNAYHMTGLTSEGLEMARAIDVALDHARVDPTDIDYVNAHGSGTRQNDRHETAAVKKSLGAHAYDTPMSSIKSMVGHSLGAIGAIEVVACVLALARQVVPPTANYETPDPECDLDYVPRTARPRRLDHVLSVGSGFGG"
  },
  {
    "reference": "MTETSVLSTRSILLIISGGIAAYKSLELIRLFRKTGTYVRVILTKGGEQFVTPLSVSALSEHPVYTDLWSLKDESEMGHIRLSREADLIIVAPASANLIAKMANGITDDLASTTLLASNKPIMISPAMNPEMWAKPSTQRNLEILKQDGIHIIAPNVGDMACGETGTGRMPEATELFKAAEDFFLNNQPLAAKTAIVTSGPTYEPIDPVRFIGNRSSGKQGHAIAAELAKQGVNVTLVTGPVSIPAPHGVKTIAIETAEDMKLAVTNALPADIFVGAAAVADWRVETPSVQKMKKRVGQTAPELKFTENPDILATVSTHPNRPSLVIGFAAETEDVLNYARAKLVKKGCDWIVANQVGKDANPVFGADDNQVTLITRTTTENWPSASKTDVAKMLVNKIVEDLKDEQQQLSRSTAK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..2. The protein contains novel Flavoprotein domains that confer a unique function or activity.3. The designed protein should have metal ion binding, phosphopantothenoylcysteine decarboxylase activity, phosphopantothenate--cysteine ligase activity, FMN binding to facilitate pantothenate catabolic process.4. The protein's Proton donor must be carefully engineered to ensure that the amino acids involved in H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2 are properly positioned for optimal catalysis.5. The protein should be optimized for activity in Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MKILLGVTGGIASYKAIDLTSKLVQKGHEVKVIMTKSAEKFVTTLPFQALSRNEVHTDIFDETDPSVIKHIDLADWADLILVAPITANTVAKMTYGIADNMLSATLLASTKPIFIAPAMNSNMLANPAVENNINILRNRGIHIIEPGAGFLACGYIAKGRMSEPLEIIENAKRALNDLGRKILITAGGTREPIDPIRFIGNRSSGKQGFALAVAAASRGAQVHLVTANTDLPTVEGITTTLVETAEEMSAVLELEFPHSDALIMSAAVADAKVANYSEAKIRKESLNRVDLAKNPDILKSLSTNKKLNQIIIGFAAETSEDLATLEENGRAKLASKSLDLIYVNNVTNGAVFGSNFTQGLDRMISVIFEGRKLAGRTVVVSAGGTAEPIDPVRVITNHSSGKMGYALAEAARDRGANVVLVAAPTNLADPVLMKVVHVRTAKQMGEAVKEYVKTADALVMAAAVADYRPLEEAGQKIKKDDDELNIALAKTDDILKSAQGNFVRVGFAAESQNLVENAKAKVSGKNLDLIAANDITAEGSGFGSDTNKVTLLFKNEKPVKLPLMSKEELSRDLLDLIREKVE",
    "response#2": "MKILLGVTGGIASYKAIDLTSKLVQKGHEVKVIMTKSAEKFVTTLPFQALSRNEVHTDIFDETDPSVIKHIDLADWADLILVAPITANTVAKMTYGIADNMLSATLLASTKPIFIAPAMNSNMLANPAVENNINILRNRGIHIIEPGAGFLACGYIAKGRMSEPLEIIEEIDSFFAKKLQGKKVLITAGPTREYIDPIRYLTNSSSGLMGCSLAAAARARGADVTLIGGPMSVRIPSGIRYIPIVSSQDLKTATLEHYDKTDVCIMSAAVADYRPSNPSAVKIKKQGKTELILNLIQNPDILSELGRRKVSQILVGFCAEDGDLLVEAPRKLQQKNCDVMVANRILPGESGFEVDKNKAWVSKNGXEKXDXLQGKKVLITAGPTREYIDPIRYLTNSSSGLMGCSLAAAARARGADVTLIGGPMSVRIPSGIRYIPIVSSQDLKTATLEHYDKTDVCIMSAAVADYRPSNPSAVKIKKQGKTELILNLIQNPDILSELGRRKVSQILVGFCAEDGDLLVEAPRKLQQKNCDVMVANRILPGESGFEVDKNKAWFISKDXENXEXXSKKEXARXLXQXIEXHFX",
    "response#3": "MKILLGVTGGIASYKAIDLTSKLVQKGHEVKVIMTKSAEKFVTTLPFQALSRNEVHTDIFDETDPSVIKHIDLADWADLILVAPITANTVAKMTYGIADNMLSATLLASTKPIFIAPAMNSNMLANPAVENNINILRNRGIHIIEPGAGFLACGYIAKGRMSEPLEIIETARILLEERLGRKILITAGGTREPIDPIRFIGNRSSGKQGFALAVAAASRGAQVHLVTANTDLPTVEGITTTLVETAEEMSAVLELEFPHSDALIMSAAVADAKVANYSEAKIRKESLNRVDLAKNPDILKSLSTNKKLNQIIIGFAAETSEDLATLEENGRAKLASKSLDLIYVNNVTNGAVFGSNFTQGLESGSRRIVLRDGKLVELPLLTKKEIAYRLLKFINGD"
  },
  {
    "reference": "MLDGIAASRCVLRGWRFTVSLRPMIKYIFITGGVVSSLGKGIAAASLAAILESRGLKVTMLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVTAKMRKSNNFTTGQIYDSVIKKERRGEYLGKTVQVIPHITNEIQAFIERGAAASYEGNADVAIVEIGGTVGDIESLPFLEAARQMNLRRGRHSTAFVHLTLVPYIASAGELKTKPTQHSVQKLREIGIFPTALLCRADRPIPDDERSKISLFSNVREEAVISVWDVDSIYKVPQMLHDQGLDRIVCEELDIKPAPADLSVWKKLIDALEHPTHEVTIGMVGKYVELTESYKSLIEALRHAGIHTLTKVNIEYIDSEVFESSDRKGLKHFDAILVPGGFGKRGTEGKIAAIRYARENKVPYLGICLGMQLAVIEYARHVAGMKDASSTEFDLETEHPVVALITEWLDRDGKVATRSADSDLGGTMRLGAQRCPIKAETLAARIYGADVNERHRHRYEVNNHYVPALEKAGMIISARTPSENLPEIMELPKAAHPWFVGVQFHPEFTSTPRDGHPLFKAFVEAAVAYKEKA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein is the optimization of the Nucleophile, active site to achieve high catalytic efficiency for H2O + L-glutamine = L-glutamate + NH4(+).2. The protein contains novel Glutamine amidotransferase, CTP synthase N-terminal domains that confer a unique function or activity.3. Ideal protein should have a binding affinity for key enzymes in the CTP from UDP: step 2/2, CTP biosynthesis via de novo pathway, Pyrimidine metabolism pathway to enhance the overall efficiency of the pathway.4. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen5. The designed protein should have a binding site that is specific to the target UTP, CTP, Mg(2+) ligand.6. The protein design must be able to perform metal ion binding in order to contribute to glutamine metabolic process, 'de novo' CTP biosynthetic process.7. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "MQDAYKSIIESFIHAGAENDARVKLHWISAEDLETEKPETLLKGMYGILIPGGFGERGVEGKINAITFARENHIPFFGICLGMQCAVIEYARNVCGITKAQSSEFHKTRFNVIDIMPDQKNIKNIGGTMRLGAYPCIIEKNTKAYEAYKSEYITERHRHRYEVNNKFRAALQSEGLIFSGLSPNRELVEMIELPGHPWFVGCQFHPELKSRATKAHPLFRDFVLQAAAGFNGKDAYKSLIEALSHGGIANKVKVNLDWIESEVFEREDPAPFLEHVNGILVPGGFGQRGAEGKIKAVRFARERRVPYFGICFGMQMAVIEAARNLVGIEGANSTEFGETKEPLVGLMTEWLRGNELEKRSKAGDLGGTMRLGAFPAALRPGSRVSKIYGNALEISERHRHRYEVNTAYKDRLEQHGLRFSGMSPDGVLPEIVEYEDHPWFVGVQYHPELKSRPLDPHPLFASFIK",
    "response#2": "MQDAYKSIIESFIHAGAENDARVKLHWISAEDLETEKPETLLKGMYGILIPGGFGERGVEGKINAITFARENHIPFFGICLGMQCAVIEYARNVCGITKAQSSEFHKTRFNVIDIMPDQKNIKNIGGTMRLGAYPCIIEKNTKAYEAYKSEYITERHRHRYEVNNKFRAALQSEGLIFSGLSPNRELVEMIELPGHPWFVGCQFHPELKSRATKAHPLFRDFVLAGATVLPDAYKSLHEALVHGGLANRVKVQVRWLDAELFETEENKDYVVAQLEPMHGILVPGGFGERGSEGKIASVQFARERNVPFFGICLGMQMACIEGARNTAGIAEASTTEFGPTAEPVVGLITEWMSKEGLQKREADGDLGGTMRLGAYPAKLAGNSVVASIYDSEDISERHRHRYEVNAHYREPLEKGGLIFSGMSPDGTLPEIVERPDHPWFVGVQFHPELKSKPFDPHPLFASFIPDAYKSLHEALVHGGLANRVKVQVRWLDAELFETEENKDYVVAQLEPMHGILVPGGFGERGSEGKIASVQFARERNVPFFGICLGMQMACIEGARNTAGIAEASTTEFGPTAEPVVGLITEWMSKEGLQKREADGDLGGTMRLGAYPAKLAGNSVVASIYDSEDISERHRHRYEVNAHYREPLEKGGLIFSGMSPDGTLPEIVERPDHPWFVGVQFHPELKSKPFDPHPLFASFIAAAWHLNQVDIALVGKYVEYEDSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASF",
    "response#3": "MQDAYKSIIESFIHAGAENDARVKLHWISAEDLETEKPETLLKGMYGILIPGGFGERGVEGKINAITFARENHIPFFGICLGMQCAVIEYARNVCGITKAQSSEFHKTRFNVIDIMPDQKNIKNIGGTMRLGAYPCIIEKNTKAYEAYKSEYITERHRHRYEVNNKFRAALQSEGLIFSGLSPNRELVEMIELPGHPWFVGCQFHPELKSRATKAHPLFRDFVLDIEKAARSIAFVGKYLDLKESYKSLTEALIHAGANLDTEVEIKWVDSEKVESEGAKKYLTDCSGILVAGGFGERGVEGKIASITYARENKIPFLGICLGMQLAMIEFARNILGIKDATSVEFNKETKNPIIYLIDEFIDAAGSTQIRTITSPMGGTLRLGEYECDTKEGSKLREAYGASTIFERHRHRYEANPLYREALEKNGMIITGESNGLIEAVEITDHPWFLGVQFHPEFTSRLQNPNPAILTFVEAAIKR"
  },
  {
    "reference": "MEVPRLSQHMSTPNSPCEEMIKNLSLENIQLCERDGNKSQDSGIAEMEELPVPHNIKISNITCDSFKISWDMDPKSKDRITHYFVDLNKKENKNSNKFKHKDVPTKLVAKAVPLPMTVRGHWFLSPRTEYTVAVQTASKQVDGDYAVSEWSEIIEFCTADYSKVHLTQLMEKAEAIAGRMLKFSVFYRNQHKEYFDYIREHHGNVMQPSPKDNSGSHGSPISAKLEGIFFSCNTEFNTGKPQQDSPYGRYRVEIPAEKLFNPNTNLYFGDFYCMYTAYHYVILVIAPMGSPGDEFCKQRLPQLSLSDNKFLTCTQEHDGLVFHQAQDVILEVIYTDPVDLSWGNVAEIIGHQLMSLSTADAKKDPSCKTCNISVGR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein have Fibronectin type-III domains with specific sequence motifs that are crucial for its activity.2. The protein must exhibit the following characteristics: May play a role in the development of the central system.. The designed protein sequence is ",
    "response#1": "PPGGIRIEEIRDTAVALTWSRGTDNHSPISKYTIQSKTFLSEEWKDAKTEPSDIEGNMESARVIDLIPWMEYEFRIIATNTLGTGEPSMPSQRIRTEGC",
    "response#2": "MPRFFQVIFVNTTSVHLVWEPPNPGAGMIRYYSINYTDSLYSQFFNFKTPNAKITTAIITGLQPATTYYFRAFAHTGRRAGAGSAVIMQDTDITVPL",
    "response#3": "PPTSVRARIDEKSAAGSAFVSWDDPNPESSSENSIDSTQKQYVINYGIYESDTQQKVRSNAKAVRLTGLIPGKEYEVAVKVVAGDGRESPWC"
  },
  {
    "reference": "MEVKAMEIFKKYLSLNIPKKILITYFLCWAGFLFSFSVGKFLLYLSSILKSNFISEPAKLAQSVGTAKFNAVSSAVSNTVGVKNAYLTYALSYIVSNFMGCLIIMFALGALAYLYKKDLEKAKTLEEKEELFKCYQKYLLILFIFTVINPLTGLIGVNLQYSDLIAVLPHGFFEFFGFATAVVVGVELSNKILPIVKREITSKKIVILIACSFIFIFIAGMLEPIDWFIYSYAKAYGIPLLAAFATGYKNLFLYLISMLFKS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be expressed specifically in the plasma membrane The designed protein sequence is ",
    "response#1": "AFHDHILMPIITAITLLVLGLLIWIVIRYNAKANPVPAKFSHNTTVEIIWTIGPVLILMVIAIFSFRLLFAYNDMPKPYLTVKATGYQWYWGYEYPDQKISEITSVPLKQEEAVAKGVPYLLAADKPMVVPVNQVIRVQVTGADVIHAFALPAFGLKTDAIPGRLNETWFKADKIGVYYGQCSELCGVDHSNMPIEIHVVSQADFDAWM",
    "response#2": "AFHDHILMPIITAITLLVLGLLIWIVIRYNAKANPVPAKFSHNTTVEIIWTIGPVLILMVIAIFSFRLLFAYNDMPKPYLTVKATGYQWYWGYEYPDQKISEITSVPLKQEEAVAKGVPYLLAADKPMVVPVNQVIRVQVTGADVIHAFALPAFGLKTDAIPGRLNETWFKADKIGVYYGQCSELCGVDHSNMPIEIHVVSQADFDAWM",
    "response#3": "AFHDHILMPIITAITLLVLGLLIWIVIRYNAKANPVPAKFSHNTTVEIIWTIGPVLILMVIAIFSFRLLFAYNDMPKPYLTVKATGYQWYWGYEYPDQKISEITSVPLKQEEAVAKGVPYLLAADKPMVVPVNQVIRVQVTGADVIHAFALPAFGLKTDAIPGRLNETWFKADKIGVYYGQCSELCGVDHSNMPIEIHVVSQADFDAWH"
  },
  {
    "reference": "MKTYNIAIVGATGAVGRELIRVLEESAFPIKTFVPLASLNSAGTFIKAFGKDYEVLETTHDIFAQAQIDIAFFSAGGSISEIFAKSAAKTALVIDNTSFFRLHLDVPLVVPEVNADEIFNAPSNIIANPNCSTIQMVHILNPLHLAFGIESVNVSTYQAVSGAGNKGIESLKSELKVALELLEQNPKAHLDKELSKGAFAYPIAFNAIPHIDVFNENGFTKEELKMVHETHKIMQASFAISATCVRVPILRSHSESLSIRFSKEVSAKEAREVLEKAPNVIVQDDPSNQFYPMALTASGSDSTFVGRIREDLFDKQTLHCFCVADQLRVGAATNALKIAWRYIERS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein should be able to manipulate the activity of enzymes in the L-threonine from L-aspartate: step 2/5, Amino-acid biosynthesis, L-threonine biosynthesis pathway, either by activating or inhibiting their function.2. The designed protein should be optimized for protein dimerization activity, NAD binding, aspartate-semialdehyde dehydrogenase activity, NADP binding activity in order to improve isoleucine biosynthetic process, lysine biosynthetic process via diaminopimelate, 'de novo' L-methionine biosynthetic process.3. The protein with the reaction L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH, the Proton acceptor must be optimized to ensure efficient catalysis.4. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.5. The designed protein should have a binding site that is specific to the target NADP(+), phosphate ligand. The designed protein sequence is ",
    "response#1": "MPTIALVGASGVVGAEFLRTMEERETPVKELRLLATSRSAGQTRRFRGEELTIEETTPAAFEGADVAFISASTAASQELCPIARDAGAVAFDDSSAFRQAPGVPLVVPEVNGVDLRDTRHIVNPTCVRVPVYYGHLHREYGIKRIVVSSYQAVSGTGKKAIDELHDQTRAILNYLDYEAKVYPHQISFNCLPHIDVFLDNGYTKEEMKMVNETRKILEDDSIGVTATTVRVPVFYGHSESVNVETKKPISAAEYRELLKSQPGVKVVDDPSKNEYPLAIDAAGQDLTLVGRIRDDESIENGINMWIVADNIRCKPIYDAVGIERINVATYQSVSGAGASGVRELEDQTHRSVRGELIDELPVHPAQMAFNVISHAWTMGEDGFSDEETKVVNETRKILEDDRLRVNPTCVRVPVYYGHSMAVHLETRESISPGAACDLLRHAPGVVLMEGAAPTPVVDAAGRDGVFVGRVRGDPSHERGLDLWVVSDNVRLLPQLSQEQIDAVKAEIAAELGD",
    "response#2": "MSIAVVGATGAVGQQIIKTLENRNFPVGKITLLSSARSAGKTALFKGEEVTIQEAKPESFEGVDIALFSAGGSVSKELAPEAVKRGAIVVDNTSAFRMDADVPLVVPEVNESALHKHRGIIANPNCSTIQMVVALEPLRQEFGLKKVIVTTMQAVSGAGNRAIQELYTQTKAVLNGEEPKAEILPVAGADKHFQIAFNPLAQIDVFEDGGYTHEEWKMIHETKKIMDDEDIQVTATCVRVPVAISHSESVWFELKQDGATVAKLQQLLSEAPGVILRDDPTRQIFPQAVQAAGQKETFVGRVRADGEQKNAFHLYCVADNLLKGAALNAVQIAETMIKEGLL",
    "response#3": "MKVAIVGASGAVGQEFLRVLDERNFPLDELVLFGSSRSAGRKYTFRGKQIEVKLLQHNDDFKGVDIAFTSAGAGTTKEFCETITKHGAVLIDNSSAYRMDADVPLVVPEVNPEDALNKYNAKNIIANPNCSTIQMVQAPIKVTASTYQAVTGSGTAAVLELTGQLEALTQEKVIPQPTVYPQQIAMNVLPQVDEFDEGNYTKEELKMRNETRKILHLPDLPITATCVRVPTMIGHAETIHVEFQESVDLQEIFSTLSRQEGISISESPHEYPTPLTAEGNDLTHIGRLRRDETVEDNKGIVFWCVSDNLRKGAALNSIQIAEVLLEPLRQEFGLKKVIVTTMQAVSGAGNRAIQELYTQTKAVLNGEEPKAEILPVAGADKHFQIAFNPLAQIDVFEDGGYTHEEWKMIHETKKIMDDEDIQVTATCVRVPVAISHSESVWFELKQDGATVAKLQQLLSEAPGVILRDDPTRQIFPQAVQAAGQKETFVGRVRADGEQKNAFHLYCVADNLLKG"
  },
  {
    "reference": "MDIEAYFERIGYQSSRNKLDLEELTEILQHQIRAIPFENLNIHCGESMELNLEVIFDQVVRKKRGGWCLQVNHLLYWALTKMGFEATMLGGYVFNTPANKYSSGMIHLLVQVTLSGKDYIVDAGFGRSYQMWEPLELTSGKDQPQVPAIFRLTEENGTWYLDQIRREQYVPNQEFVNSDLLEKNKYRKIYSFTLEPRTIEDFESINTYLQTSPASLFTSKSFCSLQTLEGVHCLVGSTLTYRRFSYKDNIDLVEFKSLTEEEIEDVLKTIFGVSLERKLVPKHGDRFFTI",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be able to bind CoA ligand in a variety of conditions2. The protein should be optimized for cytosol-specific folding and stability, while maintaining arylamine N-acetyltransferase activity, N-acetyltransferase activity.3. The protein must exhibit the following characteristics: Acetylates only arylamines.. The designed protein sequence is ",
    "response#1": "KMVGLFVSSHYKNSPNDLLLMSDAPDHHLFVLLGPIDENNTTGLPEILCAVQVSLEGEIAKESILNSIKRGYQASGDLIPWTLTQQYQDEDFPRLSGVRIVRIATHPDYQKMGYGSKALELLTQYYQGEITNLDEVNQEEEEEDEEKVDQNKGSSKISTVKKDNASLLTEVIRPKSNIPPLLFKLSERKPEKLHYMGVSYGLTQQLYQFWSKSKYLPVYLRLTSNDITGEHTCIMLRELS",
    "response#2": "KMVGLFVSSHYKNSPNDLLLMSDAPDHHLFVLLGPIDENNTTGLPEILCAVQVSLEGEIAKESILNSIKRGYQASGDLIPWTLTQQYQDEDFPRLSGVRIVRIATHPDYQKMGYGSKALELLTQYYQGEITNLDEVNQEEEEEDEEKVDQNKGSSKISTVKKDNASLLTEVIRPKSNIPPLLFKLSERKPEKLHYMGVSYGLTQQLYQFWSKSKYLPVYLRLTSNDITGEHTCIMLRELC",
    "response#3": "LTAGRGRGKSAALGLAISAAVAFGYSNIFVSSPTPENLNTLFQFVFKGFDSMEYVEHVDYELVKSTNPEFHDAIIRVNIFRSHRQTIQYIQPQDYQKLGQAELVVIDEAAAIPLPLVKNLLGPYLVFMSSTINGYEGTGRSLSLKLIKQLREQSTVVSNSSNKALNSITGRMLREIELNEPIRYSARDPIERWLNELLCLL"
  },
  {
    "reference": "MQYSIEINKNTEIFDIDKVAKQAAGAVLMRQGKSVVLATVAREEKQVEEDFLPLTVQYIEKAYAAGKIPGGYVKRETKPSDAETLTARIIDRSLRPLFPKGYTYPTQIVVMVLSADPKVDLQVMSLNAASVALYLSDIPMKAPVCGVRIGKIDGNFILNPNNEELQNSTLDLYVAGVKDELLMIEMRALPDQKENEIFIEAPYADVLTQTTSQNMNELSEDEILEALNLAQKAILNGSNAYEEAFSKHKKNSQIELKNEIEHPEILAFIENNFQKQIKEAINQMAKSERASELNKIAKEILNLEIAKDWSEESVLNTLAKVKRKLIREQILNEGKRADGRSLNEVRPISIETNILPNAHGSCLFTRGQTQALVVATLGGENDAQMIDLLTEKNPISERFMVNYNFPGFSVGEASPIKAPGRRELGHGNLAKRALYPSVDENYPYVIRLVSEILESNGSSSMATVCGGSLALKAAGVPSLKLVAGVAMGLIFEDNKHAVLTDIMGLEDHDGDMDFKVAGSKDGVTALQMDIKLGGIDQETLKQALYQAKEGRIHILNIMEEAAKEIIVNEEVLPKLELFSVDPSKIVDIIGQAGKTIKEIIEKFGVSIDLDREKGEVKIAGSQNEQIKAAKDYIINITSSQKGTKKGSKDKDISGFELGQEFQGIVKKIAPFGAFVELKNGVDGLLHSSKSKHLNLTENQSLKVKISEIKNGKISVDLCE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Involved in mRNA degradation; Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'- direction.2. The presence of S1 motif, KH domains is critical for the interaction of the designed protein with its intended targets.3. The ideal protein would be one that plays a critical role in mRNA catabolic process through RNA binding, magnesium ion binding, polyribonucleotide nucleotidyltransferase activity within cytoplasm.4. The designed protein should have a binding site that is specific to the target Mg(2+) ligand. The designed protein sequence is ",
    "response#1": "DMEVRVFAKDKIVTAMKTFDKMERNERLDNVKKEILVHFSKQYENELKSIVEVINSIIKEQFRYMITHDKVRPDEA",
    "response#2": "LQDEIKAWMGNRFLQALNNSDKAAREAATDSLRTDVLEQFSAGVAEEELAARVKAVTKNYDALVKDEVRASILEQGIRVDGRP",
    "response#3": "ELTETVRVLCETRLKELAYTPLKKEERADQTAAIYHEIIESTIEHFKATCSSEEFSTDPAKALCINPHIIDEQIHMVEKRVMRHMILDDAKRLDGA"
  },
  {
    "reference": "MAAAALLLLAAAAAAVVVAMALRWLLLLGGPAAGRLGKRARMPPGSTGLPLIGETLRLISAYKTPNPEPFIDERVARHGGVFTTHVFGERTVFSADPAFNRLLLAAEGRAVHSSYPSSIATLLGARSLLLTRGAAHKRLHSLTLTRLGRPASPPLLAHIDRLVLATMRQWEPAATVRLMDEAKKITFNLTVKQLVSIEPGPWTESLRREYVKLIDGFFSIPFPLAYFLPFTTYGQALKARKKVAGALREVIKKRMEEKAENGGSIGDDEGKKEKKDMVEELLQAEGGSFSEEEMVDFCLSLLVAGYETTSVLMTLAVKFLTETPAALAELKEEHANIRDMKGKNQPLEWSDYKSMPFTQCVINETLRVGNIISGVFRRANTDIHYKDYTIPKGCKIFASFRAVHLNNEHYENARTFNPWRWQINNKLQNAVGANIFTPFGGGPRLCPGYELARVVVSIFLHHLVTRFSWEETEEDRLVFFPTTRTLKGYPINLRLLSESIC",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization.2. The designed protein must be able to perform fatty acid alpha-hydroxylase activity under specific conditions. The designed protein sequence is ",
    "response#1": "VLHVTFSEPLYYFLHRSFHRNNYFFTHYHSFHHSSPVPHPMTAGNATLLENIILCVVAGVPLIGCCLFGVGSLSAIYGYAVMFDFMRCLGHCNVEIFSHKLFEILPVLRYLIYTPTYHSLHHQEMGTNFCLFMPLFDVLGDQ",
    "response#2": "VLHVTFSEPLYYFLHRSFHRNNYFFTHYHSFHHSSPVPHPMTAGNATLLENIILCVVAGVPLIGCCLFGVGSLSAIYGYAVMFDFMRCLGHCNVEIFSHKLFEILPVLRYLIYTPTYHSLHHQEMGTNFCLFMPLFDVLGDR",
    "response#3": "VLHVTFSEPLYYFLHRSFHRNNYFFTHYHSFHHSSPVPHPMTAGNATLLENIILCVVAGVPLIGCCLFGVGSLSAIYGYAVMFDFMRCLGHCNVEIFSHKLFEILPVLRYLIYTPTYHSLHHQEMGTNFCLFMPLFDVLGDR"
  },
  {
    "reference": "MTRRLGSGSLVIATHNVGKLKEIGALLEPYGVKCLSAGSLGLPEPAETGTTFAQNALLKARAAAEASGLAALADDSGLSVAALGGRPGVYTADWAERQWFEGAPGRDWYMAMGKVEGMLAEQGSDTDRSAAFHCVLALAWPDGEYAIYEGACHGNLSWPPRGTLGFGYDPVFVPAGRTQTFAEIPPAEKHAISHRANAFAKLVADQFG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.2. The designed protein must be able to perform dITP diphosphatase activity, XTP diphosphatase activity under specific conditions. The designed protein sequence is ",
    "response#1": "MPIGVPKVPFRSPGESDAAWFSLYKK",
    "response#2": "MNNGFKKED",
    "response#3": "MPWIDL"
  },
  {
    "reference": "MMGRPVLVLSQNMKRESGRKVQSGNINAAKTIADIIRTCLGPRAMMKMLLDPMGGIVMTNDGNAILREIQVQHPAAKSMIEISRTQDEEVGDGTTSVIILAGEMLSVAEQFLEQQMHPTVIISAYRKALDDMVNTLKEISTPVDTNDRELMLKIINSAINTKAIKLWADMACGIALDAVKTVELEENGRKEIDIKKYAKVEKIPGGIIEDSCVLRGVMVNKDVTHPKMRRLIKNPRIILLDCSLEYKKGESQTEIEITREEDFARILQMEEEYIQQVCEDIIRLKPDVVITEKGISDLAQHYLVKANITAVRRVRKTDNNRIARACGARIASRTDELREEDVGTGAGLFEIKKIGDEYFTFITDCKDPKACTIVLRGASKEILAEVERNLQDAMQVCRNVVIDPYLVPGGGASEMSVAHILTEKSKTMTGVEQWPYRAVAQALEVIPRTLIQNCGASTIRILTSLRAKHTQEGCQTWGVDGEAGVLADMKELGIWEPLAVKLQTYKTAVETAILLLRIDDIVSGHKKKGEDHGRQPAAAPEAPQQAE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must be optimized for ATP hydrolysis activity, ATP-dependent protein folding chaperone. The designed protein sequence is ",
    "response#1": "NKEIFLRELVSNASDACDKLRFEALNNAALYEDAPNLEVRVSFDKEARTLTITDNGIGMSEQEAIDHLGTIAKSGTRDFMNRLSGDQKADAQLIGQFGVGFYSGFIVADRITVESRRAGLPASEGVRWASGGAGDFEVEAIERAARGTSVILHLREDN",
    "response#2": "NKEIFLRELVSNASDAADKLRFLALSQGDLYQGDADLRVRISADKEANTITISDNGIGMTRDEVISSLGTIAKSGTAEFFKNLTGDQSKDSQLIGQFGVGFYSAFIVADLVTVRTRKAGETTAYEWESQGEGEYTLQEIEKEGRGTDIILHLREEA",
    "response#3": "NKEIFLRELVSNASDACDKLRFEALNNAALYEDAPNLEVRVSFDKEARTLTITDNGIGMSEQEAIDHLGTIAKSGTRDFMNRLSGDQKADAQLIGQFGVGFYSGFIVADRITVESRRAGLPASEGVRWASGGAGDFEVEAIERAARGTSVILHLREDS"
  },
  {
    "reference": "MVNIGVVGATGQVGQVMRTLLEERDFPAEQVRFFASARSAGRKLTFRGQEIEVEDAATADPSGLDIALFSAGATTSRAQAPRFAEAGVIVVDNSSAWRKDPEVPLVVSEVNYDRDVRGRARSLPKGIIANPNCTTMAAMPVLKPLHDEAGLVRMIASTYQAVSGSGVAGVQELYEQASAVVEGSRDLVLDGRAAQFPPPNKYVAPIAFNIVPLAGSYVDDGSGETDEDQKLRNESRKILGIPELAVSGTCVRVPVYTGHSLSLNVEFSQPLSVERAKELLASAPGVKLVDVPTPLDAAGIDESLVGRIRQDPGVPDGRGLALFVSGDNLRKGAALNTIQIAELLAAEL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein should be able to manipulate the activity of enzymes in the Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5, L-threonine biosynthesis pathway, either by activating or inhibiting their function.2. The designed protein must have a Acyl-thioester intermediate, Proton acceptor that is structurally stable and resistant to denaturation.3. The phosphate, substrate, NADP(+) binding site should be located in a region of the protein that is accessible to the ligand.4. The designed protein have Semialdehyde dehydrogenase NAD-binding domains with specific sequence motifs that are crucial for its activity.5. A protein with NADP binding, NAD binding, aspartate-semialdehyde dehydrogenase activity, protein dimerization activity is needed to enhance diaminopimelate biosynthetic process, isoleucine biosynthetic process, lysine biosynthetic process via diaminopimelate, 'de novo' L-methionine biosynthetic process, threonine biosynthetic process in a specific context.6. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.. The designed protein sequence is ",
    "response#1": "MGYVGIVGATGAVGQELIELLYKRQFPLKELRLFASSRSAGKTITWEGKSWTVENSDGQDFKGIDIVFFSAGASISKALAPVAVKGGAVVVDNSSAFRMDPDVPLVIPEINPHAIRQRPRGIIANPNCTTLSMIVAIGLAPLHKKYTMRRVIVSTYQSVSGTGVKAVQQLDNEIAGIKGEMAYPYPINRNALPHCDVFEENGYTKEEMKLAREPQKILNDKTFSVSATAVRIPTAGGHSESVNVEFENDFDLAQVRKLLHETPGIVVQDNPATNTYPMPIYAHDKDDVFVGRIRRDETQPNTLNMWIVSDNLRKGAALNAVQIAEIVAAEF",
    "response#2": "LGVLGASGAVGEVMVRLLEQRAFPLASVKFLASPRSAGKTIVFGGEKHPVIAVSDKEFEGLDILLSSTPASISKLTSPAAALAGAVVIDNSSAWRMDPTVPLVVPEVNPHDILKPIYDEVGIHRINVATYQAVSGAGRRAIEELAGQTASLLNGKGAHPEVMPKQIAFNAIPHIDTFQDNGYTREEMKMVWETQKIFDDEALAVNPTTVRIPVFYGHSEAIHVETREKMTAERARDVLRGAPGVSVIDEREAGGYPTAVSDAADADPVXVGRIREDISCXNGLCLWVVADNIRKGIIKNPSGNLKPIYDEVGIHRINVATYQAVSGAGRRAIEELAGQTASLLNGKGAHPEVMPKQIAFNAIPHIDTFQDNGYTREEMKMVWETQKIFDDEALAVNPTTVRIPVFYGHSEAIHVETREKMTAERARDVLRGAPGVSVIDEREAGGYPTAVSDAADADPVXVGRIREDISCXNGLCLWVVADNIRLNPIHKLSRIKRVVASTYQAVSGGGALAMAECIEQAKQYLAGEKVRVERFAHQIAFNLIPHIDVFQENGYTKEEMKMVLETRKILHDPEMRIAATTVRVPVLRSHSESLNIETERPLSVEEIRTALAKAPGVIVEDDPANKIYPMPLFRAERDDVSVGRIRKDLSCENGINLWVSGDQILGAETKEGLFQNDLVEWVSAMTYQAASGAGAQNMRELISQMGTIHSAVADLLADPSSAILEIDRKVAEVQRSSDFPTKNFRGVPLAGSLIPWIDVPVQNGQSKEEWKGGAECNKILGKPAFRSPGSIPIDGLCVRVGAMRCHSQALTVKLKKNVPLDEVSDIIAKANDWVKVVPNEREITERELSPAAVTGTLQIPVGRLHKLAMGDDFLGAFTVGDQLL",
    "response#3": "MALGVLGASGAVGEVMVRLLEQRAFPLASVKFLASPRSAGKTIVFGGEKHPVIAVSDKEFEGLDILLSSTPASISKLTSPAAALAGAVVIDNSSAWRMDPTVPLVVPEVNPHDIDLTRHKGIIANPNCTTMAAMPVLEPIRQQWGLKRIIVSTYQAVSGAGVGAIMETQEQLRAVLNDGLNPKDLEAKILPSGGDKKHYPIAFNALPQIDVFTDNDYTYEEMKMTKETKKIMEDDAIAVSATCVRIPVLSGHSESVYIETKEIADITAVKKAIANFPGAVLEDDVAHQIYPQAVNAVGSRDTFVGRIRKDLDKENGIHMWVVSDNLLKGAALNAVQIAELVAAEFTA"
  },
  {
    "reference": "MSRQAPLVFIMAAGLGTRMKSDTPKVLHRVAGRPLLHWVVTAARAAGAGRVVAILGHKAEVVKTALDADFGKGSVEIAIQPEQRGTGHAVQCALPAVQNEPDDRIVLILTGDAPLLASERIAELTAACEASAAGLALLSTIPPRPMPYGRLVRDPGGTLERIVEHSDATEEQKRIADTNAGFYAIRLGHLRKDLAALRADNAKGELYLTDLVAHAHKRGGATSIDAPFTEVAGINDRTDLAGVEVEARRRINEAWMRAGVTMVDPASTYIDADVGPLGKDTWLGPNVALRGKTSLGAGVRVDLGCVLTDVQVAENTNIKPYSILTETAVGPRAQVGPFSHCRPGSRLDEDVHLGNFVETKKTHLMAGSKANHLAYLGDASVGSKANIGAGTITCNYDGVGKYKTTIEAGAFIGSDSQLVAPITIGRDAYVGSGTTVTKDVPRGALAITRVKQVNVEGWADRFREANAKRKKGEGEP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should aim to mimic or enhance the function of natural proteins involved in UDP-N-acetylglucosamine biosynthetic process, lipid A biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.2. The designed protein should contain one or more MobA-like NTP transferase domains that are essential for its function.3. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+), acetyl-CoA binding.4. A protein that is capable of catalyzing reactions within the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "VLVLAAGKGTRMRSSRPKVLHRLAGCPLVEHVLRAADPLAAASTTLVVGHQAEAVERELAVSRGGLRFARQQRQLGTAHAVLQAEPMLAGKHGTLIVLMGDAPLVAAGTAQALAETHERTGAAATVLTATLEVRAPGGYGRLVRDAAGEVARIVEQKDASAEELAIDECNTGIMAAPTARFREWLGALRNDNAQGEYYLTDIVALAVRDGLAVVPAQPAPEAEVLGVNDRRQLAECERDWQRRRAAALMEAGVTMRDPGSAWIDAGVTIGRDTVLYPHVSLEGTTTVGEGTTIRSGVRITDCVIGNNVEILDHCVLRGSHVEDEAHVGPFAHLRPGAIMRRKAKVGNFVEMKKAELGEGSKANHLTYLGDARIGKGVNIGAGTITVNYDGVNKHQTVIEDHASVGSDTQIIAPITIGQGAIIAAGTTVTQDVPADSLAIARVPQRTLEGWKRPGVRLADPARLDVRGSLRCGQDVEIDVNCVFEGEVFLGDGVRIGAHCVIANARIEPGAVIHPFTHIDGERLGVTVGESALVGPFARLRPGSVLGPQVHIGNFVEVKNATLATGAKANHLAYLGDATVGERVNYGAGSITANYDGAHKHRTVIEADVHVGSNCVLVAPVTLGAGGTIGGGSTITKDTPPGALTVARAKKRPAQPR",
    "response#2": "VVVMAAGKGTRMKSARPKVLHRLAGKALLGHVIERAAGLGARQAVIVTGHQAEQVEAALAAMNEQRLPWRCVRQSPQLGTGHAVQQAMPALPDDGVTLVLSGDVPLTEVATLQALLDACAGQRLSLLTAHLPDPTGYGRIVRDEQGQVTRIVEQKDASEAERALSEVNTGILAAPTEQLKRWLAALKNDNAQAEYYLTDIVAQAVADGVAVHGAQPADPWETLGVNSKAQLAELERIHQRNLADRLLEAGVALADPARIDVRGTLRCGHDVQIDVGCVFEGDVELGEGVHIGPYCVIRNSHIGAECRIDAHSVIDGAVLAGLNNVGPFARLRPGSRLAVRARVGNFVETKNAEVGEGSKINHLSYIGDARLGRDVNVGAGTITCNYDGANKHRTDIGDEAFIGSNSALVAPVSIGKAATIGAGSTISRDVADHSLAVGRSRQVTIAGWQRPVKPKKP",
    "response#3": "VIILAAGQGTRMKSSLPKVLHSVAGRPLLEHVVRAAQVLEPLAIHVVVGHGAEQVQAALADYPIRWVLQREQLGTGHAVLQAIPAVAESSTVLVLYGDVPLIGADTLRKLADKAGSAPAGLGRVVRDAAGALLGIVEHKDASPEQRAIAEINSGIYAFDAAVLRDALGRLGRDNAQGEVYLTDVLAIARAEGLSVGALVAADHRETLGVNDRAQLAAAGAELNRRTLEAWMRAGVTMVAPDTSTIDDTVELQADVIIEPQTHLRGNTSIGAGSRIGPGTLIENSQIGENVSVLYSVISDSIIESNSRIGPYAHLRGEAKVGAGCRVGNFVELKKTTIGQKTNVAHLTYLGDATVGEQVNVGAGTITANYDGVKKHPTTIGDRSKIGSNSVLVAPVNLGEDVTVAAGSVVTEDVADDSLVIARSRQRNIEGWVARRRAGTKTAEAAERAAQQAQSSQPPHR"
  },
  {
    "reference": "MKTLYLRNVPDDVVERLERLAELAKTSVSAVAVRELTEASRRADNPALLGDLPDIGIDTTELIGGIDAERAGR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: The cognate toxin is VapC9.; Antitoxin component of a possible type II toxin-antitoxin (TA) system.2. The protein design should aim to mimic or enhance the function of natural proteins involved in regulation of DNA-templated transcription, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "LLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSVAATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVI",
    "response#2": "LLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSVAATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVV",
    "response#3": "LLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSVAATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVQ"
  },
  {
    "reference": "MFKKVSKNANRLSRHQRVRNKITGTPERPRLNVYRSLTNIYVQLIDDVAGKTLVAASSLDKEIKDQVSATGNAEAAKLVGQLVGKRALEKGIDTVTFDRGGNIYHGRIQALAEGAREAGLKF",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Prioritize Basic residues compositional bias within the protein sequence to achieve desired characteristics.2. The protein must exhibit the following characteristics: This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance..3. A protein that can perform structural constituent of ribosome in ribonucleoprotein complex, ribosome to support translation. The designed protein sequence is ",
    "response#1": "GPIACKVTLRGERAEKVLKMFLEGIGNRLKASQFDEYGNVSMGIDEHIDIPGMKYDPEIGIFGMNLSVTFEKPGHRINRRRIQRRKVPQKHRVSREEAIEV",
    "response#2": "PIGCKVTLRGEAAEGFLETSLGIVEKRLNESQFDIFGNVSFGVEEHTDYPGMRYDPNIGIFGMDITVVVNRPGYRVSKRRIAKRKIPTSHKITKEDTIIG",
    "response#3": "PIACKVTLRGERAEKVLKMFLEGIGNRLKASQFDEYGNVSMGIDEHIDIPGMKYDPEIGIFGMNLSVTFEKPGHRINRRRIQRRKVPQKHRVSREEAIEA"
  },
  {
    "reference": "MGGLGSPCGGCKFLRRKCVEGCVFAPYFCYEEGSSNFAAIHKVFGASNFSKLISHLPDHDRCDAVRTISYEAHSRLHDPIYGCVSQIFSLQQQVVSLQAQVVLLREEASRRFPQDDPGCNMKQQEKVLAQQMPQDLHNWFHQGILDSDFDPMSGTTRDNERSMDHNESPCSSNESLNYYQEVNFPWSV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must have at least one LOB domain.2. The designed protein must be able to regulate positive regulation of DNA-templated transcription.3. The protein design should emphasize Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "PVARDHTHGRAAKFRTSANIWMNEAMRAAGYEPYNVSMSNHDIERGNRYFYFAKDLTIPYRNDPVSDNTGFVFCDVDYYADMEKWMQHFKPMLLYTLVPESLSYHCDDHSFHVNDDRVFFDVRGGASYSHQLWDYTGDTICVV",
    "response#2": "RINMVAATVSTHSSNRLKAFIKAEAYAATNDPRNITTMAPELTLMMSCFTYAFKEKILYEQPWYGPGKTPKQVGRRLQSIAKHGTLESDYSRFDGSISEWLQKNVVKAAYMRFFKEHQRTEFQSWFSKVFMQMGTTTAGVRYEAGWGTRSGSPITTDGNTMLNAFVVYCCYRKLCHTPAEAWRKLSQGALLTGDDAVLAHENGLEPALLDVVKNLGLKVEAKVNGPDDPVSFCGRIQ",
    "response#3": "RINMVAATVSTHSSNRLKAFIKAEAYAATNDPRNITTMAPELTLMMSCFTYAFKEKILYEQPWYGPGKTPKQVGRRLQSIAKHGTLESDYSRFDGSISEWLQKNVVKAAYMRFFKEHQRTEFQSWFSKVFMQMGTTTAGVRYEAGWGTRSGSPITTDGNTMLNAFVVYCCYRKLCHTPAEAWRKLSQGALLTGDDAVLAHENGLEPALLDVVKNLGLKVEAKVNGPDDPVSFCGRIG"
  },
  {
    "reference": "MAYAHLFTFSNFTFLRGASHPAEMVEQAYRLGYDAIALTDECSLAGAVKAHVMAEECGIKLIVGSYFKLSNNCELIALAPDRAAYAELSGFITLARRRADKGEYTAHMDDLRFRLQTCLIIWLPTANTATEDNARIFSAAFKQRLWLGVGHQLAGGEQRLFQQWQNLAATYQLPMVASHLALMHSAERKPLQDVLTAINHNTSVSQLGTRLQSNGENYLKAIDEVFYLYPPGLIKQTLLIAERCNFSLNELKYQYPQELVPKGLSPIAHLRALVDSGKARRWPGGVPAQAEAILAKELALIEELHYEYYFLTVYDIVAFARQQNILCQGRGSAANSVVCYCLFITEIAPGQINVLFERFISKERDEPPDIDVDFEHQRREDVIQYIYTKYSRQRAALAATVISYRSRSAIRDVGKALGLDPALVDHLAKSLAWWDRTGDLVKRIEAAGVQTERQLVQQFFALVQQIIGFPRHLSQHVGGFVITQDKVSDLVPVENASMPGRTVIQWDKEDLEAMGLLKVDILALGMLTALRKTLAMVNRYEPAIASLADIPPEDPHTYDMLCAADTVGVFQIESRAQMSMLPRLRPRTFYDLVIEIAIVRPGPIQGDMVHPYLRRRDGLEPVSYQSPDIADVLKPTLGVPIFQEQAIRLAMVAAGFSGGEADRLRRAMASWGKNGNLLQFEETFIQGMLNNGYELDFAHRLFEQIKGFGGYGFPESHSASFAILCYASSWLKCHHPAAFYCALLNSQPMGFYSPSQLIQDARRHGIPV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm that is critical for DNA replication, DNA repair.2. The protein must exhibit the following characteristics: It is not the major replicative DNA polymerase.. The designed protein sequence is ",
    "response#1": "MLGCLRRGFELLHTVYQTRMDLAAIPAEDPQTYRMVQKADTLGTFQIESRAQMSMLPRMKPATFYDLVIQVAIVRPGPIQGDMVHPYLRRREKLEPVTYPSETLRGILGKTLGVPLFQEQAMQVAIHCAGFTPGEADQLRRAMATFKMTGGVSPFRDKLVNGMLANI",
    "response#2": "MLGCLRRGFELLHTVYQTRMDLAAIPAEDPQTYRMVQKADTLGTFQIESRAQMSMLPRMKPATFYDLVIQVAIVRPGPIQGDMVHPYLRRREKLEPVTYPSETLRGILGKTLGVPLFQEQAMQVAIHCAGFTPGEADQLRRAMATFKMTGGVSPFRDKLVNGMLANL",
    "response#3": "MLGCLRRGFELLHTVYQTRMDLAAIPAEDPQTYRMVQKADTLGTFQIESRAQMSMLPRMKPATFYDLVIQVAIVRPGPIQGDMVHPYLRRREKLEPVTYPSETLRGILGKTLGVPLFQEQAMQVAIHCAGFTPGEADQLRRAMATFKMTGGVSPFRDKLVNGMLANI"
  },
  {
    "reference": "MADGAPARCYALITSAGNGSRAVRPADPAAPKQYQRLAGQRVIEHTLAAFLALPHWAGVAVVVAPGDDAYQSSDARVRVWPVGGAVRAQTVHNGLQQLAAAGARDSDWVLVHDAARCLIEPAQIERLLAACWDDPVGGLLALPLPDTLKSATAATAATAAMPRVAATLERDHKWLAQTPQMFRLGALRAALAAHQGSGYAGITDEASAIERSGAQPLLVPGSASNFKITYPADFALAEALLAQRQTQRLALPDSATAATPTTAPMNPASPAPATPAPNPLAGLRIGEGWDIHALVPGRPLILGGIHIPHPSGLLGHSDADALLHAITDALLGAAALGDIGTLFPDTDARFAGADSAQLLAQAMQRVAEHGYALINLDTTVIAQAPKLAPYKAAMQARIAEVLGLQPGQVNVKAKTAEKLGPVGQGLAIECRAVALLQRVA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The stability of the protein-a divalent metal cation complex should be optimized to maximize enzymatic activity.2. The 4-CDP-2-C-methyl-D-erythritol 2-phosphate binding site should be located in a region of the protein that is accessible to the ligand.3. The protein should be designed to have high stability and activity towards 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-   erythritol 2,4-cyclic diphosphate + CMP.4. The protein should be able to modulate terpenoid biosynthetic process in a way that leads to a desirable outcome.5. For general function, the protein need meet that Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D- erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C- methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF). The designed protein sequence is ",
    "response#1": "MRPGQGFDIHRLVPGRRLVLGGIDIPFDHGLLGHSDADVLTHAVIDAMLGAAGLGDIGQHYPDTDPAYAGASSMVLLERAVGMLQAAGYLVSSVDSVVIAERPRIGPFVPQMRESLARAMKVGPGCVNIKGKTTEGLGPMGTGEAIAALAVAMVVEPRAVLDALAAAVMEQAGADVNAVDGDAENTKITTPEDFQEADRKMIASQSVRTGMGFDVHRFEPGDAVTLCGVSIPHTARLAGHSDADVGLHALTDAILGAIAAGDIGDHFPPSDPQWKGAASDRFLRHAADLVAARGGAVTHVDVTLICEKPKIGPHKEAMRMTIAGILGVDIDQVSVKATTTEKLGFTGRGEGIAAQAVASVL",
    "response#2": "MRPGQGFDIHRLVPGRRLVLGGIDIPFDHGLLGHSDADVLTHAVIDAMLGAAGLGDIGQHYPDTDPAYAGASSMVLLERAVGMLQAAGYLVSSVDSVVIAERPRIGPFVPQMRESLARAMKVGPGCVNIKGKTTEGLGPMGTGEAIAALAVAMLETALGRGRVRVGQGFDVHPFAPGDHLWVCGVRIPHGMSLAGHSDADVGLHALTDAVLGAIGAGDIGMHFPPSDPRWRGAASDQFLTRAADLVRTRGGAVAAVDVTIICERPKIAPYRAAMVERIAGILGITPDRVSVKATTTEKLGFTGRAEGMAAQAVATVRPRVGVGIDVHAFDDSSPLWLAGLHWPGERGLSGHSDGDPVAHAVCDALLSAAGLGDIGSRFGTADPRFQDAHGEVFLRATRDLVEAAGFTIGNVSVQLIGNRPRFSPRREEAESLLSALLAAPVSVAATTSDALGFTGRGEGVTAVATALVLETVPE",
    "response#3": "MGEAEMSRTRAMGGLGPPLMRPGQGFDIHRLVPGRRLVLGGIDIPFDHGLLGHSDADVLTHAVIDAMLGAAGLGDIGQHYPDTDPAYAGASSMVLLERAVGMLQAAGYLVSSVDSVVIAERPRIGPFVPQMRESLARAMKVGPGCVNIKGKTTEGLGPMGTGEAIAALAVAMVSELLG"
  },
  {
    "reference": "MPENIIAGIPVHFPFEPYEVQRAYMEKVIMCLRDGTNGVLESPTGTGKTLSLLCASLAWIRTRQSEQQQYMQKLQLDQQKQATGGAGAGVADLNAVMGKANNWGVPKVIYASRTHSQLSQAMRELKRTAYANMKSVVLGSRDQLCIHPDVMREQGNSNKVNMCKLKVHAKTCSFQLRVESKKDHPDFRGPSIMDIEDLVKVGQRLKMCPYFASKELVNGADITFMPYNYLLDPKARKANKIELSNTIVILDEAHNIEKICEESASVQIRSSDVAMAIEDITHIMKIFTSGDAQDTGGPEEPKDFTLDDLTLLKEMLLELEKAIDGVVVDNKAEGTTYPAAHIYELLGKANFTYGNCATIVALLDKLVQYLMVASQHSTMLRKGGSFMVLADLLNIVFANKEDVMSKVHRSFKVHVEIEDNKQSKNAANSAKPQTGWLGKGNNAASSVTKAAKIINFWCFNPGFGMEQLLNAHVRSVILTSGTLAPLKPLIAELAIPVAQHLENPHIVDRSQVYVKIIGTGPDREQLISNYKNRDNPKYISSLGQTILNVSRIVPDGLLVFFPSYPMLNQCVDAWQASGLWADISCRKPIFLEPRGKDQFTSTMEEFYQAIRDSKGACFMAVCRGKVSEGLDFADRNGRAVIITGLPFPPLKDPKVVLKRRYLETNRTKENQLLSGQEWYNLDATRAVNQAIGRVIRHRNDYGAILLCDARFQDMSQVQQLSKWIRGHLGARPQSSPFGPIVRELRQFFKHAEQTMAQPEERVVEPLLQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The presence of Helicase ATP-binding domains is critical for the interaction of the designed protein with its intended targets.2. For general function, the protein need meet that Acts as an anti-recombinase to counteract toxic recombination and limit crossover during meiosis; ATP-dependent DNA helicase implicated in DNA repair and the maintenance of genomic stability; Regulates meiotic recombination and crossover homeostasis by physically dissociating strand invasion events and thereby promotes noncrossover repair by meiotic synthesis dependent strand annealing (SDSA) as well as disassembly of D loop recombination intermediates.3. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP, [4Fe-4S] cluster binding.4. The ideal protein would be one that plays a critical role in regulation of double-strand break repair via homologous recombination, negative regulation of response to DNA damage stimulus, DNA recombination, male germ-line stem cell population maintenance through DNA helicase activity, ATP binding, metal ion binding, ATP hydrolysis activity, DNA binding within nucleus.5. The protein design should emphasize Polar residues compositional bias.6. We are looking for a protein with broad substrate specificity for ATP + H2O = ADP + H(+) + phosphate.7. The protein design should include a DEAH box motif to ensure proper function. The designed protein sequence is ",
    "response#1": "NGVTVDFPFQPYKCQQEYMTKVLECLQQKVNGILESPTGTGKTLCLLCTTLAWREHLRDGISARKIAERAQGELFPDRALSSWGNAAAAAGDPIACYTDIPKIIYASRTHSQLTQVINELRNTSYRPPARLLAPSYAWATFLRLGHVSVTLASSGRRGSCVSPYGSRCRATLWVLETAPPRPTVLSPTRPKVCVLGSREQLCIHPEVKKQESNHLQIHLCRKKVASRSCHFYNNVEEKSLEQELASPILDIEDLVKSGSKHRVCPYYLSRNLKQQADIIFMPYNYLLDAKSRRAHNIDLKGTVVIFDEAHNVEKMCEESASFDLTPHDLASGLDVIDQVLEEQTKAAQQGEPEELRLPEYASRTHSQLTQAISELRNTSYRPRVCVLGSREQLCIHPEVKKQESNHMQIHLCRKKVASRSCHFYNNVQNGVTVDFPFQPYKCQEEYMTKVLECLQKRVNGILESPTGTGKTLCLLCTTLAWREHLRDTISAHKIAERVQGELFLDRALSSWGNAAATEGDPIACYTDIPKIIYASRTHSQLTQVIGELRNTSYRPKVCVLGSREQLCIHPEVKKQESNHVQIHLCRRKVASRSCHFYNNVEGKSLEQELATPILDIEDLVKSGSRHRVCPYYLSRNLKPQADIIFMPYNYLLDAKSRRAHNIDLKGTVVIFDEAHNVEKMCEESASFDLTPHDLASGLDAVDQVLEEQARVAQQGEFHPL",
    "response#2": "NGVTVDFPFQPYKCQEEYMTKVLECLQKRVNGILESPTGTGKTLCLLCTTLAWREHLRDTISAHKIAERVQGELFLDRALSSWGNAAATEGDPIACYTDIPKIIYASRTHSQLTQVIGELRNTSYRPKVCVLGSREQLCIHPEVKKQESNHVQIHLCRRKVASRSCHFYNNVEGKSLEQELATPILDIEDLVKSGSRHRVCPYYLSRNLKPQADIIFMPYNYLLDAKSRRAHNIDLKGTVVIFDEAHNVEKMCEESASFDLTPHDLASGLDAVDQVLEEQARVAQQGEFHQREGVKFMYDCLMGLARPTIENPDIDCTTKSLVLENDSDISGCLLADDMGLGKTLMSITLIWTLIRQTPFASKVSCSQSGIPLTGLCKKILVVCPVTLIGNWKREFGKWLNLSRIGVLTLSSRNSPDMDKMAVRNFLKVQRTYQVLIIGYEKLLSVSEELEKNKHLIDMLVCDEGHRLKNGASKILNTLKSLDIRRKLLLTGTPIQNDLNEFFTIIDFINPGILGSFASFKRRFIIPITRARDTANRYNEELLEKGEERSKEMIEITKRFILRRTNAILEKYLPPKTDIILFCKPYSQQILAFKDILQGARLDFGQLTFSSSLGLITLLKKVCNSPGFLMDLLKKLRDEGHQTLVFSQSRRILNIIERLLKNRHFKILRIDGTITHLVEREKRISLFQQNKDYSVFLLTTQVGGVGLTLTAASRVVIFDPSWNPATDAQAVDRVYRIGQKENVVVYRLITCGTVEEKIYRRQVFKDSLIRQTTGDKKNPFRYFSKQE",
    "response#3": "VTVDFPFQPYKCQEEYMGKVLECLQKKVNGILESPTGTGKTLCLLCTTLAWREHLRDSISARKIAQRAHGGLHTLSSWGDAAPDGDSTACYSDIPKIIYASRTHSQLTQAISELRNTSYRPRVCVLGSREQLCIHPEVKKQESNHMQIHLCRKKVASRSCHFYNNVEGKRGLVKATCWSLSVQPGGRGVWPPACPCPSRACWLQPCVLSLVLQSRRAHGIDLKGTVVIFDEAHNVEKMCEESASFDLTPQDVASGLGAVDQVLEEQTKAANGVTVDFPFQPYKCQEEYMTKVLECLQKRVNGILESPTGTGKTLCLLCTTLAWREHLRDTISAHKIAERVQGELFLDRALSSWGNAAATEGDPIACYTDIPKIIYASRTHSQLTQVIGELRNTSYRPKVCVLGSREQLCIHPEVKKQESNHVQIHLCRRKVASRSCHFYNNVEGKSLEQELATPILDIEDLVKSGSRHRVCPYYLSRNLKPQADIIFMPYNYLLDAKSRRAHNIDLKGTVVIFDEAHNVEKMCEESASFDLTPHDLASGLDAVDQVLEEQARVAQQGEFYPVMEKSLEFWRAHDLVRRMEELKPLFVEPRSKGSFSEVINAYYQRVSSPGSSGATFLAVCRGKASEGLDFSDTNGRGVIVTGLPYPPRMDPRVVLKMQFLDEMKGQGEPGGQVLSGQKWYQQQASRAVNQAIGRVIRHRHDYGAVFLCDHRFALYASRTHSQLSQVISELRSTTYRPKVCVLGSREQLCINPEVRKIESNHIQIHVCRKKVSTHSCHFYNNVEEKSTEKELVTPILDIEDLVKGGNRHKVCPYYLSRTLKQQADIIFMPYNYLLDSKTRRAHNIDLKGTVVIFDEAHNVEKVCEESASFDLT"
  },
  {
    "reference": "MSSTGVKTMKDFMLNKSNIRNMCVIAHVDHGKSTLTDSLVTLAGIISNEKAGVARYTDTRPDEQERCITIKSTSISMYYEIEDKEDIPADANGNGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVEGVCVQTETVLRQALTERVKPIVIINKVDRVILELKEEPEEAYQSFCRSIENVNVLISTYKDELLGDVQVSPGEGTVAFGSGLHGWAFTLEKFAKMWSAKFGIDRKRMLEKLWGDNYWDAKAKKWKKNGKGDHGEVLQRGFVQFCFDPITKLFNAIMEGRKADYEKMLTNLQIKLSADDKEKEGKELLKTVMKLWLPAGVTLLEMIVLHLPSPVVAQKYRTSNLYTGPMDDEAAKAMANCDEKGPLMMYVSKMIPTNDKGRFYAFGRVFSGTIRTGGKARICGPNYVPGKKDDCVIKNIQRTMLMMGRYTDPIDECPCGNVIGLVGVDQYLLKSGTITDSVAHIIKDMKFSVSPVVRVAVETKNPSDLPKLVEGMKRLSRSDPLCLCYTEESGEHIVAGAGELHLEVCLKELQEDYCSGVPLIVTEPVVSFRETITEPSRIQCLSKSANNQNRLFMRAFPFPEGLAEDIEAGEIKPDTDFKERAKFLSEKYGWDVDEARKIWCFGPDNCGPNLFVDVTKGIQYLNEVKDSIVNGFNNAMHDGVVCNEQIRGVRINLEDVKLHADAIHRGGAQMIPCARRCCFACVLTGAPSLLEPMYLAEIQCPESAIGGIYTVMSRRRGKIISEEQRPGTPLFNVRA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a high specificity for GTP binding, with minimal off-target effects.2. The protein should have translation elongation factor activity, GTP binding and be active in cytoplasm.3. The protein contains novel tr-type G domains that confer a unique function or activity.4. For general function, the protein need meet that Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.; During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively; Catalyzes the GTP-dependent ribosomal translocation step during translation elongation The designed protein sequence is ",
    "response#1": "KMRNIGISAHIDSGKTTLSERILFYCDRIHALHEVRGKDGVGATMDNMELEKERGITIQSASTQVKWKDYTVNVIDTPGHVDFTIEVERSLRVLDGAILVLCSVGGVQSQSITVDRQLKRYHVPRIAFVNKCDRTGANPFKVRMQLREKLGLNAYMMQIPIGLEDKLEGVVDLVTMKAMYFEGENGTQIRMAEIPQHLLADAQKYREEMIDAATMFSDELAEAFLEGAETEEMIRAAVRKGTLAEQFVPVFLGSAYKNKGIQPLLDAVGYYLNTRNIGIMAHIDAGKTTTTERILYYTGKIHKIGETHEGASQMDWMEQEQERGITITSAATTAQWAGHRVNIIDTPGHVDFTVEVERSLRVLDGAVTVLDAQSGVEPQTETVWRQATTYGVPRIVFINKMDKTGADFLYSVGTLHDRLQANAHPIQLPIGAEDQFSAIIDLVNTRNIGIMAHIDAGKTTTTERILYYTGKIHKIGETHEGASQMDWMEQEQERGITITSAATTAQWAGHRVNIIDTPGHVDFTVEVERSLRVLDGAVTVLDAQSGVEPQTETVWRQATTYGVPRIVFINKMDKTGADFLYSVGTLHDRLQANAHPIQLPIGAEDQFSAIIDLVEREPARDGKLCFFRVYSGKIEAGSYVYNPVKGKKERVGRILQMHANHREERNVVYTGDIAAAVGLKNTSTGETLCAPDNPLLEPIQKIQITVPSEQMGAATSQIQGRRGQVFDMLSEGDTMTIVGKAPVAELFGFAGDIRSATEGRAMWSTEFAGFELVPAGIVR",
    "response#2": "KMRNIGISAHIDSGKTTLSERILFYCDRIHALHEVRGKDGVGATMDNMELEKERGITIQSASTQVKWKDYTVNVIDTPGHVDFTIEVERSLRVLDGAILVLCSVGGVQSQSITVDRQLKRYHVPRIAFVNKCDRTGANPFKVRMQLREKLGLNAYMMQIPIGLEDKLEGVVDLVTMKAMYFEGENGTQIRMAEIPQHLLADAQKYREEMIDAATMFSDELAEAFLEGAETEEMIRAAVRKGTLAEQFVPVFLGSAYKNKGIQPLLDAVGYYLNTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETHEGASQMDWMEQEQERGITITSAATTAQWNGHRVNIIDTPGHVDFTVEVERSLRVLDGAVAVLDAQSGVEPQTETVWRQATTYGVPRVVFVNKMDKIGADFLYSVKTLHDRLQANAYAIQLPIGAEDDFKGIIDLVEPRELLEPIQKIQITVPSEQMGAATSQIQGRRGQVFDMLSEGDTMTIVGKAPVAELFGFAGDIRSATEGRAMWSTEFAGFELVPAGIVKPGNVPIYREAITQRADFNYTHKKQTGGSGQFGRVAGFIEPCTEQDYEFDNQIKGGAIPSEFIPSCDKGFKEAIKRGTLIGFPIVGTKVTINDGQSHPVDSSDMAFQAAAIGAFREAYKAAEPVMKVEVETPEDYMGDIMGDLNRRRGMVQGMEDLPGGTKQIRAEVPLAEMFGYATQMRSMSQGRATYSMEFQKYAEIKEPIMKLEAVTPEEYMGDVIGDLNSRRGQIISVEPSPETTVITGTVPLAESFGYTTDLRSVTKGRATFSMEFESYREMK",
    "response#3": "KDYRNIGIMAHIDAGKTTTTERILYHTGKIHKIGETHDGVSQMDWMEQEKERGITITSAATTAYWKNKRINIIDTPGHVDFTVEVERSLRVLDGAVAVLDAQSGVEPQTETVWRQATNYKVPRIVYVNKMDKAGADFEAAVASVKSRLGGNAVAIQWPIGSESNFNGIIDLVTMTATTYNGESAEEEFPMEIPTDLLDVAKAKRQELLEAAANFDEEVMMMVLEGADVDIDTFKNTIRKATLTSEFFPVVCGTSFKNKGVKKMIDAVVDYLPEPQAITDVDEQTRNIGIMAHIDAGKTTTTERILFYTGRVHKLGETHDGASTMDFMDQEQERGITITSAATTCQWRDTRVNIIDTPGHVDFTVEVERSLRVLDGAIGVFCAKGGVEPQSETVWHQADRYHVPRIAYINKMDIMGADFYNVVDMMNERLQANPVPIQLPIGKEDDFQGIVDLIQTRNIGIMAHIDAGKTTTTERILFYTGRVHKLGETHDGASTMDFMDQEQERGITITSAATTCQWRDTRVNIIDTPGHVDFTVEVERSLRVLDGAIGVFCAKGGVEPQSETVWHQADRYHVPRIAYINKMDIMGADFYNVVDMMNERLQANPVPIQLPIGKEDDFQGIVDLIQTRNIGIMAHIDAGKTTTTERILFYTGRVHKLGETHDGASTMDFMDQEQERGITITSAATTCQWRDTRVNIIDTPGHVDFTVEVERSLRVLDGAIGVFCAKGGVEPQSETVWHQADRYHVPRIAYINKMDIMGADFYNVVDMMNERLQANPVPIQLPIGKEDDFQGIVDLIKNTAVIYKDDLGTEWEEVEIPEDMQELAEEYRDKMLEMAAEYDEELMMKYLEGEEITSEEIRLALREGCLKTELTPVLCGSSYKNKGVQMLLNAVIDYLQTRNIGIMAHIDAGKTTTTERILFYTGRVHKLGETHDGASTMDFMDQEQERGITITSAATTCQWRDTRVNIIDTPGHVDFTVEVERSLRVLDGAIGVFCAKGGVEPQSETVWHQADRYHVPRIAYINKMDIMGADFYNVVDMMNERLQANPVPIQLPIGKEDDFQGIVDLI"
  },
  {
    "reference": "MRLLLDLHPDDYPLEGYRRRQLLDWVFGQGVGSFAAMANLPAELRAELERDYLLNPFREIETVRSTDGSVKYLFTLQDGRQMEAVYMPYLDRKTICVSTMVGCPAKCAFCATGAMGFGRNLTPGEVVGQILAVAGGEGLAPRELRNLVFMGMGEPLLNYEHTMNAARILLHPLALGMSKRRITLSTVGLAKGIRRLAAEDDLGLKLAISLHAPDEETRQRIIPTGAANGISEIMAAAREYQAVTGRRLTFEYSMLRGVNDHPWQAELLADLLSGLVSHVNLIPMNPWDGSGFESSTEAEIQAFYDALEARGVDVSVRRSRGKDAGAACGQLALKRPNAARGEARFGAA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A key design requirement for the protein is the optimization of the Proton acceptor, S-methylcysteine intermediate to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.2. The protein can be expressed in cytoplasm and perform metal ion binding without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "MRPSLIGLSKAGLAETLVSHDVVSEKEGRMRASQLWNWLYVNGVTDFERMTNVAKPVRQKLSDTFNLDRPEIVSEQVSVDGTRKWLFRFRDPANPNLPAVEVETVYIPESDRGTLCVSSQVGCTLTCSFCHTGTQKLVRNLTAGEILGQILMARERLGDFPGGSRPDDGGLVPGGETRAITNIVMMGMGEPLYNYENVKQALLIASAGDGMSISKRRITLSTSGVVPYIEPTGREIDVMLAISLHAVRDDLRDVLVPINKKWPLKELLEACKTYPGLSNARRITFEYVMLDGINDSDAEARELVRLLAGIPAKINLIPFNPWPGSNYGTSPSSRIERFADIVNKAGYASPVRTPRGRDIFAACGQLKSESERLSRKAVA",
    "response#2": "MRPSLIGLSKAGLAETLVSHDVVSEKEGRMRASQLWNWLYVNGVTDFERMTNVAKPVRQKLSDTFNLDRPEIVSEQVSVDGTRKWLFRFRDPANPNLPAVEVETVYIPESDRGTLCVSSQVGCTLTCSFCHTGTQKLVRNLTAGEILGQILMARERLGDFPGGSRPDDGGLVPGGETRAITNIVMMGMGEPLYNYENVKQALLIASAGDGMSISKRRITLSTSGVVPYIEPTGREIDVMLAISLHAVRDDLRDVLVPINKKWPLKELLEACKTYPGLSNARRITFEYVMLDGINDSDAEARELVRLLAGIPAKINLIPFNPWPGSNYGTSPSSRIERFADIVNKAGYASPVRTPRGRDIFAACGQLKSESERLSRKAA",
    "response#3": "MRPSLIGLSKAGLAETLVSHDVVSEKEGRMRASQLWNWLYVNGVTDFERMTNVAKPVRQKLSDTFNLDRPEIVSEQVSVDGTRKWLFRFRDPANPNLPAVEVETVYIPESDRGTLCVSSQVGCTLTCSFCHTGTQKLVRNLTAGEILGQILMARERLGDFPGGSRPDDGGLVPGGETRAITNIVMMGMGEPLYNYENVKQALLIASAGDGMSISKRRITLSTSGVVPYIEPTGREIDVMLAISLHAVRDDLRDVLVPINKKWPLKELLEACKTYPGLSNARRITFEYVMLDGINDSDAEARELVRLLAGIPAKINLIPFNPWPGSNYGTSPSSRIERFADIVNKAGYASPVRTPRGRDIFAACGQLKSESERLSRKNR"
  },
  {
    "reference": "MAKQIVYSEASRQAILRGVNQLADAVKVTLGPKGRNVVLEKKFGGPTITKDGVTVAKEIELKDPLENMGAQMVREVASKTSDVAGDGTTTATILAQSIYREGVKAVAAGANPMALKRGIDKAVELATEEVKKLSKPVSGDMIAQVGTISANSDKTIGNIIADAMKKVGKDGVITVEESKTMVTELDTVEGMQFDRGYLSPYFVSDAERMEAVLEDPYILIHEKKISNMKDLLPLLEQIARSGKPLLIIAEEVEGEALATLVVNKLRGTLNACAVKAPGFGDRRKAMLEDIGILTGGKPIMEDIGVKLEGVRLEDLGRAKRVTVDKDNTTIVDGAGNPKGIEGRIKQLRAQIDETTSDYDREKLQERLAKLAGGVAVIKVGAATETEMKEKKARVEDALHATRAAVEEGIVPGGGVALLRAAKALATFKVDGDEQIGVSIVKRACEEPLRQIVSNSGTEGAIVVDKVRENANNNYGYNAATDTYEDLVAAGVIDPTKVTRSALQHAASIAGLMLTTEAMIAEIPEKKSAPAGGPGGHGPEMDY",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. We are looking for a protein with broad substrate specificity for ATP + H2O + a folded polypeptide = ADP + phosphate + an   unfolded polypeptide..2. For general function, the protein need meet that The GroEL-GroES system forms a nano- cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.; Together with its co-chaperonin GroES, plays an essential role in assisting protein folding3. A protein that contributes to protein refolding by functioning in cytoplasm with isomerase activity, unfolded protein binding, ATP binding, ATP-dependent protein folding chaperone.4. The protein should have a high affinity for ATP. The designed protein sequence is ",
    "response#1": "MPIGVPKVPFRSPGESDASWVDIYRRLYRERLLFLGQEVDSEISNQLIGLMIYLSIEDDTKDLYLFINSPGGWVIPGIAIYDTMQFVVPDICAAVQEGIVVGGSFILVGGEITKRXXAXAXLMEAYLGGXGGXFXEVGVQANEILQLKKLXNVYXSXTGKPLWVVSEDMERDVFMSATEAQTHGIVDLIAVEE",
    "response#2": "MKRILLATHNPHKVAEFQALLQGFDFELLPQSEVNIDEAEETGLSFAENAILKARHAARITGLPAIADDSGLCVDALDGRPGIFSARYAGPDASDAENNAKLLHDLAGAPAERRTARFHCVLVLMRHADDPEPLIADGEWEGHILEQPRGEGGFGYDPLFWLPERQKTAAELDPALKNRLSHRAKALAQLRQRLAA",
    "response#3": "MIKRLFKTN"
  },
  {
    "reference": "MRGRPVPRPTRPHRSRERDTVKSEENKVRTDSTSESENPAIPSPTADTSQRPRTNRDWWPNQVDLSVLNKPSKDSDPLGADFDYKAEFAKLDVEALKQDLRRLMRTSQDWWPADWGHYGPLFIRMSWHAAGTYRIADGRGGGGQGAQRFAPLNSWPDNASLDKARRLLLPIKQKYGRTLSWADLLVLAGNVAHDDMGLPTFGFAFGREDVWQPEEVFWGPEDTWLGDERYAGEEPLDLDEGPLANVTMGLIYVNPEGPKGNPDPLASAHDIKVTFRRMGMTDEETVALIGGGHTFGKTHGAGNPELIGPEPEGCPVHGNGLGWISQYGTGKGADTITSGLEGAWTPTPTQWDNSYWDTLFGLDWELTESPAGAKQWQPSNPEAQELVPDAHIEGKKNPPMMSTADVALKVDPELRRYAERFRGDPEYFQDTYARAWFKLLHRDMGPKSRYLGPDVPAEDLIWQDPVPAVDHELVGEAEVAALKQRLLASGLTVSQLVHTAWSAAASYRGTDKRGGANGARLRLEPQIGWAVNAGVRDVLPVLERVKGEFEQQTGTKVSLADLIVLGGTAAVEKAAADAGVPVTVPFSPGRTDASQEQTDVENFGWLEPRADGFRNWIVPDTKIAPETLLVDKAYMLELTPKEMTVLVGGLRVLGANTGGVPHGVFTDRPGVLSQDFFQNLLDLGISWSTSAETEGVYEGRDADGTVVRTATAADLVFNSNSILRGIVEVYSSDDAKEKFVQDFAAAWVKVMELDRFDLR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein have Plant heme peroxidase family profile domains with specific sequence motifs that are crucial for its activity.2. The designed protein should be optimized for heme binding, metal ion binding activity in order to improve hydrogen peroxide catabolic process.3. For general function, the protein need meet that Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.4. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.5. The protein should be designed to have high stability and activity towards AH2 + H2O2 = A + 2 H2O.6. The stability of the protein-heme b complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "FQWQDPTPKGKKISSATANSIKKAALKSGLSVSELVKVAWASASTFRSTDMRGGANGARIALAPQKSWEVNNPKLLEKALPKLEKISKRYGASLADTIVLGGAAAIEKAARDAGVKVKVPFKSGRGDATQEQTDITSFKFLEPKADAFRNYYTEESGRTPIYMLIDKANMLGLTVPEMTVLVGGMRVLDANADGSKHGVFTKRPGRLSNDFFVNLLTMDNKWVKSSMPGIYEGRDRKTNELKYTATPVDLVFGSHAELRAVAEVYASL",
    "response#2": "FQWQDPTPKGKKISSATANSIKKAALKSGLSVSELVKVAWASASTFRSTDMRGGANGARIALAPQKSWEVNNPKLLEKALPKLEKISKRYGASLADTIVLGGAAAIEKAARDAGVKVKVPFKSGRGDATQEQTDITSFKFLEPKADAFRNYYTEESGRTPIYMLIDKANMLGLTVPEMTVLVGGMRVLDANADGSKHGVFTKRPGRLSNDFFVNLLTMDNKWVKSSMPGIYEGRDRKTNELKYTATPVDLVFGSHAELRAVAEVYASLE",
    "response#3": "FQWQDPTPKGKKISSATANSIKKAALKSGLSVSELVKVAWASASTFRSTDMRGGANGARIALAPQKSWEVNNPKLLEKALPKLEKISKRYGASLADTIVLGGAAAIEKAARDAGVKVKVPFKSGRGDATQEQTDITSFKFLEPKADAFRNYYTEESGRTPIYMLIDKANMLGLTVPEMTVLVGGMRVLDANADGSKHGVFTKRPGRLSNDFFVNLLTMDNKWVKSSMPGIYEGRDRKTNELKYTATPVDLVFGSHAELRAVAEVYASY"
  },
  {
    "reference": "MSTPIALVPARTAEVTRNTAETRITVKVNLDGSGQSHLSTGIGFFDHMLDQIARHGLIDLDIQATGDLHIDGHHTVEDVGITLGQAVHQAVGDKKGIRRYGHAYVPLDEALSRVVIDFSGRPGLVMDVPFKSGMIGTFDAQLAHEFFQGFVNHAFVTLHIDNLKGENAHHQAETVFKAFARALRAALEFDPRALGQIPSTKGTL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be designed to catalyze the following reaction D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-   4-yl)-2-oxopropyl phosphate + H2O.2. Ideal protein should have a binding affinity for key enzymes in the L-histidine biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "GIDAHHTVEDIGITV",
    "response#2": "GIDAHHTVEDIGITGL",
    "response#3": "MXGVDYHHLIEDLGIVL"
  },
  {
    "reference": "MATHLVVLAAGQGSRMNSDLPKVLHPLGGVPLFVHALAAGAALDPDRRILVVGHGAGAVRRAAARHDDGLIVVEQAERLGTGHAVRQALGALEGAEGDVFVLYGDTPFIRPHTLQAMRDRRAAGAALVVLGFEAADPGRYGRLIADGDRLERIVEWKDATEAERAVTLCNSGVICADASQLPGLLAAVTNDNAAGEYYLTDIVGIARSRGLDAALVTCPEAETLGINTRAELAAAEAAFQARARAAAIAEGATLLAPETVFFAQDTHLGRDCTVEQFVVFGPGVTVETGAHVRAFSHLEGCHVGQGAVVGPYARLRPGAEIGNDARIGNFVEVKAAEIGEGAKVNHLSYIGDAAVGARANIGAGTVTCNYDGVFKHRTEIGADAFIGSDTMLVAPVNVGCGAMTASGSVITEDVAPGALALGRARQVNKDGFARRLMDSLRDAKARKAR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.2. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).3. We are looking for a protein with broad substrate specificity for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.4. The designed protein should have magnesium ion binding within cytoplasm to regulate peptidoglycan biosynthetic process, UDP-N-acetylglucosamine biosynthetic process, lipid A biosynthetic process, regulation of cell shape.5. The designed protein should have a binding site that is specific to the target UDP-N-acetyl-alpha-D-glucosamine ligand. The designed protein sequence is ",
    "response#1": "VVVMAAGKGTRMKSSKPKVLHRLGGRALAQHVIDTAARVSARSVVVITGHGAEQVEAGLVAAEGMALQFVRQEPQLGTGHAVQQAVPVLADDGVTLVLSGDVPLMRPETLQALLDLCGDEVGLLTDIVLAAGQGTRMKSTTPKILHPLAGAPIIAHVLATARALDAKHVVAVVRHERDLVADAIQAELPEAVIVDQDEVAGTGRAVEQAIAALPDDFDGDVLVLNGDVPLLNASTLAEMLERHRADGLAASVLSARYDDPSGYGRIVRGPEGAFDRIVEQKDASADELAIDEINAGVYAFGASALRDQLANDPSGYGRIVRGADGSVLRIVEEKDATDAERAIGEINTGILVAPTAALKRWLSALSNDNAQQEYYLTDIIALAVADGVPVVTAHPVDADQVAGVNSPSQLAALERAHQLRQARALMDAGVTIEDPATTWIDLAVRIEPDVTIRPGTQLKGATAIATGAVIGPDTTLVDCEVGEGAEVKRADATQAVIGAGASVGPFAYLRPGTVLDADGKIGTFVETKNAHIGAGSKVPHLSYIGDTEIGEGSNVGAGSITANYDGVEKHRTTIGSHVRTGSHGVFVAPVTIGDGAYTGAGTVVRKDVPAGALGVNVAPPATAIPGWKRPVKAAK",
    "response#2": "IVLAAGQGTRMKSTTPKILHPLAGAPIIAHVLATARALDAKHVVAVVRHERDLVADAIQAELPEAVIVDQDEVAGTGRAVEQAIAALPDDFDGDVLVLNGDVPLLNASTLAEMLERHRADGLAASVLSARYDDPSGYGRIVRGPEGAFDRIVEQKDASADELAIDEINAGVYAFGASALRDQLANEASGDFYLTEIISMYKTHPEKLIKLTSNNNELKGVNSKLQLNELEAIQTERLRIRFINEFMDDK",
    "response#3": "VVVMAAGKGTRMKSSKPKVLHRLGGRALAQHVIDTAARVSARSVVVITGHGAEQVEAGLVAAEGMALQFVRQEPQLGTGHAVQQAVPVLADDGVTLVLSGDVPLMRPETLQALLDLCAQPHTVAMLSVEPTAPDGLGRIVRRGERVVEIVEQKDASAEERAVREVNAGVYVFRAAELRRHLSRLTTDNAQGELYLTDVLSIARADGHSVAAHRADDEGQLLGVNDRVQLAEARALMNRRILERWMRAGVTIEDPATTWIDLAVRIEPDVTIRPGTQLKGATAIATGAVIGPDTTLVDCEVGEGAEVKRADATQAVIGAGASVGPFAYLRPGTVLDADGKIGTFVETKNAHIGAGSKVPHLSYIGDTEIGEGSNVGAGSITANYDGVEKHRTTIGSHVRTGSHGVFVAPVTIGDGAYTGAGTVVRKDVPAGALGVNVAPPPRIEGWVERKRPGSAAAEAARRAREGDGE"
  },
  {
    "reference": "MALVEDNNAVAVSFSEEQEALVLKSWAILKKDSANIALRFFLKIFEVAPSASQMFSFLRNSDVPLEKNPKLKTHAMSVFVMTCEAAAQLRKAGKVTVRDTTLKRLGATHLKYGVGDAHFEVVKFALLDTIKEEVPADMWSPAMKSAWSEAYDHLVAAIKQEMKPAE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that May not function as an oxygen storage or transport protein, but might act as an oxygen sensor or play a role in electron transfer, possibly to a bound oxygen molecule; Has an unusually high affinity for O(2) because of a very low dissociation constant.2. The protein must be able to metal ion binding. The designed protein sequence is ",
    "response#1": "RFFSLILEIAPVAKNMFSFLKDSDELPENNPKLRAHAVKVFKMTCESAIQLREKGEVVVGETTLKYLGSIHLQKRVADPHFEVVKEALLRTVKEATGNKWKDEMKEAWSEAYDQLASAM",
    "response#2": "WQDDLNIGIDVIDQQHRRIIEMLNHLHVAQASMQRAAVGEVIDEVVDYTMSHFAFEEELMEEAGYPFCAAHKRVHEVFIKRVSEYRMRFQAGEDISDELRTMLSRWLFNHIRGDDQAYAES",
    "response#3": "RFFSLILEIAPVAKNMFSFLKDSDELPENNPKLRAHAVKVFKMTCESAIQLREKGEVVVGETTLKYLGSIHLQKRVADPHFEVVKEALLRTVKEATGNKWKDEMKEAWSEAYDQLASAL"
  },
  {
    "reference": "WIIPFVPLPVPMLIGVGLLLFPIXTNKLRRMWAFPSILLLSIVMIFSTNLSIQQINSNSIYQYVWSWTLDNDFSLELGCLIDPLTSIMSMLITIVGITVLIYSDNYMAHDQGYLRFFAYMSFFSTSMLGLVTSSNLIQIYIFWELVGVCSYLLIGFWFTRPLAANACQKAFVTNRVGDFGLLLGILGFYWITGSFEFRDLFEIFNNLIVNNQVNYLFVTLCAALLFAGAVAKSAQFPLHVWLPDAMEGPTPISALIHAATMVAEGIFLVARLLPLFIVIPYIMNFISLIGIITVLLGATLALAQKDIKRGLAYSTMSQLGYMMLALGMGSYRSALFHLITHAYSKALLFLGSGSVIHSMETIVGYSPEKSQNMVLMGGLRKYVPITKISFLLGTLSLCGIPPLACFWSKDEILNDSWLYSPIFAIIAWATAGLTAFYMFRIYLLTFEGHLNVYFQNYSGKKNTAFYSISIWGKGCSKRINKNFRLLRINNQSSSFFLKKTYRSDENLKKRNGGRPFINLIRFENKKARLYPYESDNTMLFPLLILVLFTLFVGYLGISFNQEARDLDILSKWLAPSIDLLHQKSRDLTDWYEFLKDAIFSVSIAYFGILLASLLYKPIFASFKNFDLINSFVKTGPKRSRWDKILTLLYNWSHNRAYIDVFYTTSFTGSIRGLSQLTHFFDTQVIDGITNGVGVMSFFV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The designed protein sequence is ",
    "response#1": "RLDHPYIRKNGKLVEVSWSEALKTIMDKIKSVKPEKIAAIAGSIVSVEAMFMLKILLQKLGSNNYTVNQFNYKIDTSERGNYLFNTTIVGVEKADLCLLIGANIRQIAPILNSRIGRRVRIGALKVVRIGIGHNQTYKIHDLGNDIKIIEDLAVGTHEYTKAFKEAKYPMIIVGDGVYGRDDGYALLSLIHKVVDKYNMMRDDWQGFNILHNHASIVGGLDIGFNTAIKFEGVKLAYLLGADAIPFDKLKSAFIIYQGHHGDVGAMSADVILPSAAYTEQSGIYVNLEGRPQIAQKAVSPVGRAKEDIEIIKEIADEIARKK",
    "response#2": "RLDHPYIRKNGKLVEVSWSEALKTIMDKIKSVKPEKIAAIAGSIVSVEAMFMLKILLQKLGSNNYTVNQFNYKIDTSERGNYLFNTTIVGVEKADLCLLIGANIRQIAPILNSRIGRRVRIGALKVVRIGIGHNQTYKIHDLGNDIKIIEDLAVGTHEYTKAFKEAKYPMIIVGDGVYGRDDGYALLSLIHKVVDKYNMMRDDWQGFNILHNHASIVGGLDIGFNTAIKFEGVKLAYLLGADAIPFDKLKSAFIIYQGHHGDVGAMSADVILPSAAYTEQSGIYVNLEGRPQIAQKAVSPVGRAKEDIEIIKEIAEIARLSKFYMHESCGQCTPCREGTGWMWRVMMRLVKGNVKKSEIDELLNVTKEIEGHTICALGDAAAWPIQGLIRHFRSEIEAGA",
    "response#3": "RLDHPYIRKNGKLVEVSWSEALKTIMDKIKSVKPEKIAAIAGSIVSVEAMFMLKILLQKLGSNNYTVNQFNYKIDTSERGNYLFNTTIVGVEKADLCLLIGANIRQIAPILNSRIGRRVRIGALKVVRIGIGHNQTYKIHDLGNDIKIIEDLAVGTHEYTKAFKEAKYPMIIVGDGVYGRDDGYALLSLIHKVVDKYNMMRDDWQGFNILHNHASIVGGLDIGFNTAIKFEGVKLAYLLGADAIPFDKLKSAFIIYQGHHGDVGAMSADVILPSAAYTEQSGIYVNLEGRPQIAQKAVSPVGRAKEDIEIIKEIAEIARLSKFYMHESCGQCTPCREGTGWMWRVMMRLVKGNVKKSEIDELLNVTKEIEGHTICALGDAAAWPIQGLIRHFRSEIEAL"
  },
  {
    "reference": "MKVMGTQRNCQHLLRWGIIILGIIMICSTTDNLWVTVYYGVPVWKEAPTTLFCASDAKSYETEAHNIWATHACVPTDPNPQEINLENVTENFNMWKNNMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTKNATLEQEGMINCSFNITTEIRDRKKQVYSLFYKLDVIQIGNNSSNDSYRIINCNTSAITQACPKVKFDPIPIHYCAPAGYAILKCNNKTFSGKGPCRNVSSVQCTHGIRPVVSTQLLLNGSLAEEDIIIRSENLTANTKTIIVQLNESVTINCTRPNNNTRKSVRIGPGQVFYTTDVIGDIRQAFCTINRTEWDKTLGQVAKKLSKLFNNKTIEFKPHSGGDLEITTHSFNCRGEFFYCNTSNLFKGIWNGKWNGTNSTESYINITCRIKQIINMWQRAGKAMYAPPIKGIVNCISNITGLLLTRDGGVNSTNETFRPGGGNMRDNWRSELYKYKVVRLEPLGLAPTRAKRRVVEREKRAIGLGAMFLGFLGAAGSTMGAASLTLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGVKQLQARVLAVERYLKDQQLLGIWGCSGKHICTTTVPWNSSWSKNKSLEQIWNNMTWMEWEREIDNYTDLIYSLIEKSQTQQEKNEQELLQLDQWASLWNWFSITNWLWYIRIFIMIVGGLIGLRIVFAVLSIINRVRQGYSPLSFQTLAPNPGGLDRPERIEEEGGEQGRTRSTRLVSGFLALAWDDLRSLCLFSYHHLRDFILIAARTLNLLGH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein contains novel Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains that confer a unique function or activity.2. The protein's functional design necessitates the inclusion of a YXXL motif; contains endocytosis signal motif.3. A protein that contributes to fusion of virus membrane with host endosome membrane, mitigation of host antiviral defense response, positive regulation of plasma membrane raft polarization, clathrin-dependent endocytosis of virus by host cell by functioning in host cell endosome membrane, viral envelope, host cell plasma membrane, virion membrane, plasma membrane with structural molecule activity.4. The designed protein should contain Cytoplasmic topological domains.5. For general function, the protein need meet that Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Transmembrane protein gp41: Acts as a class I viral fusion protein; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes6. Incorporate a signal peptide in the protein design.7. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "MRVKGIRKNYQHLWRWGTMLLGMLMICSATEKLWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWVTHACVPTDPNPQEIVLGNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDANVTYNTTDNLGTQEMKNCSFNITTSVRNKMKREYALFYSLDIVPIDNDNTRYRLRSCNTSIITQACPKVSFEPIPIHFCAPAGFAILKCNNKTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSENFTNNAKTIIVQLNESVEINCTRPNNNTRKSIHIGPGRAFYTTGDIIGDIRQAHCNISRTNWTNTLKRVAEKLREKFNNTTIVFNQSSGGDPEIVMHSFNCGGEFFYCNTTQLFNSTWNETNSEGNITSGTITLPCRIKQIINMWQEVGKAMYAPPIGGQIKCLSNITGLLLTRDGGSDNSSSGKEIFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLRDQQLLGLWGCSGKLICTTSVPWNSNWSNKSEKEIWDNMTWMQWDREIDNYTNAIYRLLEESQNQQEKNEKDLLALDSWNNLWNWFNISNWLWYIKIFIMIVGGLIGLRIIFGVLSIVKRVRQGYSPLSFQTLTHHQGEPDRPGRIEEGGGEQD",
    "response#2": "MKVKGTRKNYQHLWRWGTMLLGMLMICSAAEKLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPADPNPQELELKNVTENFNMWKNDMVNQMHEDVISLWDQSLKPCVKLTPLCVTLNCSNVSSNSSGTSNLGTQEMKNCSFNITTSVRNKMKREYALFYSLDIVPIDNDNTRYRLRSCNTSIITQACPKVSFEPIPIHFCAPAGFAILKCNNKTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSENFTNNAKTIIVQLNESVEINCTRPNNNTRKSIHIGPGRAFYTTGDIIGDIRQAHCNISRTNWTNTLKRVAEKLREKFNNTTIVFNQSSGGDPEIVMHSFNCGGEFFYCNTTQLFNSTWNETNSEGNITSGTITLPCRIKQIINMWQEVGKAMYAPPIGGQIKCLSNITGLLLTRDGGSDNSSSGKEIFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRAVGIGALFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQRNLLMAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTTVPWNTSWSNKSMDVIWNNMTWMEWEREIENYTGVIYTLIENSQNQQEKNELELLELDKWASLWNWFDISNWLWYIKIFIMIVGGLVGLRIIFAVLSIVNRVRQGYSPLSLQTHLPARRGPDRPEGIEEEGGERDRDTSGRLVDGFLAIIW",
    "response#3": "MKARGIRNNYLWRWGTMLLGMLMICSATEQLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPADPNPQELELKNVTENFNMWKNDMVNQMHEDVISLWDQSLKPCVKLTPLCVTLNCSNVSSNSSGTSTNTSNLWVTVYYGVPVWKEASTTLFCASDAKAYDTEVHNIWATHACVPTDPNPQEIFLNNVTEDFNMWKNSMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDLNCTDSKNNCTKLRNTTTSSNASSTVEKGEIKNCSFDITTSIRTQVKDYAFFYKLDVVPINNDNISYRLINCNTSVITQACPKVSFEPIPIHYCTPAGFVLLKCNDKKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVIRSSNFSDNAKNIIVQLNQSVEIDCTRPNNNTRKRVTLGPGRVWYTTGQIIGDIRKAYCTLNKTKWENTLKQVVEKLRKQFGNKTIIFNQSSGGDPEIVMHSVNCGGEFFYCNTSRLFNSTWKTNSTGNNNSTWNDTITLPCRIKQIVNMWQEVGKAMYAPPIAGRIRCISNITGLLLTRDGGNNKTNTETFRPGGGNMKDNWRSELYKYKVVQIEPVGVAPTRAKRRVVQREKRAITLGAMFLGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLALERYLRDQQLLGIWGCSGKLICTTNVPWNTSWSNKSLSYIWDNMTWMEWEREIDNYTGLIYNLIEESQNQQEKNELELLKLDSWASLWNWFDISKWLWYIKIFIMIVGGLIGLRIIFTVLSIVNRVRQGYSPLSL"
  },
  {
    "reference": "MTDTHGTFIPTADLVDIIGDDVRSCDTQFRNLGGRTDFHGTITTVKCFQDNALLKSILGEDNPGGVLVIDGDASLHTALVGDIIAGLGRDHGWSGVVVNGAIRDSAVIGEMEFGCKALGTNPRKSSKTGAGERDVVVSFGGVDFTPGHYLYADSDGIVVTENPVKAPASN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The substrate binding site should be located in a region of the protein that is accessible to the ligand.2. The protein design must be focused on enhancing the efficiency of regulation of RNA metabolic process.3. The stability of the protein-a divalent metal cation complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "MNILLSNDDGYHAEGIQTLATRLREAGHCVTVIAPDRNRSAASSCLTLMEPIRVHQLGTFDYSVIAGTPADCVHLALNGLFETSFDLVVSGINHGANLGDDVVYSGTVAAALEGRHLRLPSLAVSLVGKQSEGHLFGNNHFETAAQVVLDVLPKLVDMALPRQILNINVPDLPYSAIKGMLVTRLGQRSPSAEILKSQDPRGSTIYWLGENGSAIDNGEGTDFYALAHDYVSITPIHADML",
    "response#2": "PQPSLETHKYHRGHALVAGGEVMTGAARLAARAAARLGAGLVTVAAPEAAFPIYAAALTGVIVHPIADIEGFQGFLADKRRNAALIGPGAGVGDETRARTLAILGAGKRAVLDADALTSFAEDPGTLFSAIRSPCVMTPHAGEFSRLFDAAGDKPERARRAARQSGAVVLLKGADTVIAAPDGRVAVNANAPPQLATAGSGDVLAGIVLGLLAQDMDPFAAAAAAAWIHGAAASRFGLGLVAEDLIDAVVPVE",
    "response#3": "ILVTNDDGVRAPGIRSLAEALRNIGDVVVVAPDRERSAVGHALTLHHPLRASEIRPAVFAVDGTPTDCVNLGIHTLLGSRPDIVVSGVNCGGNMGDDITYSGTVSAAMEATLMGIPALAVSLATSGRGDNYAVASAFAARLVRIVSERGLPPDTLLNVNVPDLPLEKLGGAVVTIQGKRDYN"
  },
  {
    "reference": "MTRTTHFGFKTVSETEKAREVAGVFDSVAERYNLMNDLMSGGMHRLWKRFAIEVSGVRTGERVLDIAGGTADLSSLFLEQVGSRGEVWLTDINNSMLTIGRDRLLDEGTAVPVAQCDAEKLPFPTNYFDCVSVAFGLRNMTHKDVALKEMLRVLRPGGCVIVLEFSRIWKPLQRLYDLYSFKVLPAMGGLVAKDRDSYRYLAESIRVHPSQEELKQMMQKAGFERVQYFNLAANAVALHRGYKF",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be optimized for S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity, S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity, 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity, S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity, demethylmenaquinone methyltransferase activity activity in order to improve menaquinone biosynthetic process, ubiquinone biosynthetic process, aerobic respiration.2. We need a protein that can perform a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine with high efficiency.3. The S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MRSGSRSATGTAARPRWSSASARSRRCPAARSAPPGSRGSSTPPRSAPTLSRTVPDAVPATTPAPQPAGAAAPADLDLDAVRAEVAAHRARVEELGVDLVAYGSRKTQRIPEAAAKGPEAMEAHERELLARFRDAYFTANGVHYDLMNDLMSGGLHRLMGLLGAPVAGLDVAGTRPDGRPVKLLTGPGTTTPVRYTGTFEDAWRVDTALAAERPELGEVPVFLRHTTPCGIAVGATAREAWDRAHEADPTSAFGGVIAVNRPVDAATAELIGGTFLEVIVAPDYEPEALDVLAAKKNIRLLAARRPRADRDAAAEPVSVRSVLGGLLVQDRDAVPAGEEQWTVATERPTEGQLADLVLAWKVVGSVKSNAILLTAADRTIGVGAGQMSRVDSTRIAARKAEAAGDATRGAVMASDAFFPFPDSVEAAAAVGVRAVIQPGGSVRDDEVIAAADEHGIAMVFTGVRHFRH",
    "response#2": "MSILSGRPSPVIPDLTTNIARLNQLKVADLFSVPENDLNQLTGNQLRYDFLNHLLSLRRDISERAKNDLRFPTPGGGTQLFHALFADNPDLDMVCLMSGLSGTGKTTLERQVPGVIDLDTYRHQRGALIGSTPGQGSQDTQKMAFQHQLLHQSLRVYRQQKVACFVESTCSSKAMRDLFCSMAQQSGYRVVILHLLISLEIAQQRVQDRVWRTVPQAVFDRQWAQLTGYVNQGLDLVEQLLANPGCQQVTVEGKLGDVIAPLHQEYFAHRQS",
    "response#3": "MSQTHFGYESVPENEKAGRVRDVFHSVAGKYDIMNDLMSLGIHRLWKRFTIELSGVRSGNRVLDIAGGTGDLAAKFSRIVGSTGEVVLADINESMLKVGRDRLIDRGVVGNIRYVQADAESLPFPDNHFDCITIAFGLRNVTDKDAALRSMQRVLKRGGRFLCLEFSKPTNPLLSKVYDTYSFQVLPWLGRMVAGDADSYRYLAESIRMHPDQETLKTMMEDAGFARVGYRNLSGGVVALHRGYKL"
  },
  {
    "reference": "MIHPIEAIIEDLQNGKPVIMVDDEGRENEGDLIFPAQFATKSLVNFAVKEARGLLCVAITPERAQALELPPMEKRNTDPHGTAFTVSVDAASSTTGISAAERAVTIRLLAESSTAPADLRRPGHVFPLVAREGGVLRRAGHTEATVDLLRLAGLEPVGALIEIMKEDGEMARLAELEAFAERHGLKIGTIESLIRYRLERGDGFVTREAEATLPTEFGEFRIVGYRDRITGEEHAALVMGELDPEEPVLVRMHSECLTGDALHSLRCDCGFQRDLAMKRIAEEGRGVLVYLRQEGRGIGLVNKIKAYHLQDSGLDTVEANLALGFPPDLRDYGVGAQILYDLGVRKLRLLTNNPRKIRAMGGYGLEVVERVPLRSGDNEHNARYLRAKQEKLGHWMD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be optimized for activity in 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The Nucleophile; for GTP cyclohydrolase activity active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "VEVAMPTAYGSFRLIPFRQKSNGLEHVVLIKGQWEPDEPILVRVHSSCVTGDIFGSMRCDCGDQLHKAMEMIEKEGKGALVYMNQEGRGIGLMNKIKAYHLQENGLDTVEANIHLGFEADERDYGVGAQILREIGVTNMRLMTNNPVKRIGLESYGLKIVEIVE",
    "response#2": "RVASVHLPTDFGDFTAIGYANDVDDKEHIALVKGEIDPEKPVMVRVHSECLTGDVFGSHRCDCGPQLHAALSQIDREGTGVLLYMRQEGRGIGLLNKLKAYELQEKGLDTVEANLKLGFRPDLRDYGIGAQILRDLGVRKMRLLTNNPRKIAGLKGYGLEIVEVLE",
    "response#3": "MFTGIITGIGRVIDRIPDPAKGVERLIFSAPGHVEGLTLGGSIAVNGVCVSAVEITGETLTAELIPETLQRTTFGDLHTGDQVNLERCLPSGARLDGR"
  },
  {
    "reference": "MNELANFANPLASTEHFDHIQISLASPERIRSWSFGEIKKPETINYRTFKPERDGLFCARIFGPIKDYECLCGKYKRMKYKGIVCEKCGVEVTVSKVRRERMGHIELAAPVAHIWFLKSLPSRIGLLLDMQLKQLERVLYFESYIVIEPGLTPLKKFQLLTEDELLEAQDQYGEDSFSAGIGAEAVKKLLEALDLETEREDLLEELKHTKSELKPKKIIKRLKVVESFIESGNRPEWMILEVIPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLMDLRAPDIIVRNEKRMLQEAVDALFDNGRRGRTITGGNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVITTGPELKLHQCGLPKKMALELFKPFIYSRLDAKGLSMTLKQAKKWVEKERKEVWDILEEVIREHPVMLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCSAFNADFDGDQMAVHVPLSLEAQLEARVLMMSTNNILSPANGKPIIVPSQDMVLGIYYLSMLKENEPGEGMRLSNMTEVHQALNVGAVTLHSKIISRVPQVNEQGETYMKRVETTPGRMLLGETLPQNYKVPFETINRLLTKKDIADVIDTVYRHTGQKDTVLFADAIMSLGFKYACKAGISFGKDDMIVPAAKEALVEETRALVQDFEQQYQDGLITQQEKYNKVIDAWSRCGDRVAGEMMKEIQMVHKGPDGRELPVNAIYMMAHSGARGSAAQIKQLAGMRGLMAKPSGEIIETPIISNFKEGLTVLEYF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be optimized for magnesium ion binding, DNA-directed 5'-3' RNA polymerase activity, zinc ion binding activity in order to improve DNA-templated transcription.2. We need a protein that can perform a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +   RNA(n+1) with high efficiency. The designed protein sequence is ",
    "response#1": "EWMVMRVIPVIPPELRPLVPLDGGRFATSDLNDLYRRVIIRNNRLKRLIDIKAPEVILRNEKRMLQEAVDSLFDNSRKSNSVRGSSNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVSGPHLELHQCGLPKEMAVELFKPFIIRRLIERGIVKTVKSAKKYVDKKTEDVWDILEKVIQGRPVLLNRAPTLHRLGIQAFQPVLTENKAIEIHPLVCPAYNADFDGDQMAVHVPLSHEACLESMVLMLSSHNVRSPADGGPLAVPSQDMILGLYYIL",
    "response#2": "EWMIMTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPTLKLHQCGLPKKMALELFKPFIYGKLERRGMATTIKAAKKMVEREMPEVWDVLDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIHLHPLVCAAYNADFDGDQMAVHVPLTIEAQLEARALMMSTNNILSPANGEPIIVPSQDVVLGLYYML",
    "response#3": "RKIASVRVSVASPEIIRAWSSGEVKKPETINYRSFKPERDGLFCEKIFGPTKDYECACGKYKGKKYEGTVCERCGVRVESKEARRKKMGHIELAAPVVHVWYLKSSPSILSTLLNIPARDLENIVYHGSRRIIERSYIITDSE"
  },
  {
    "reference": "MTETLINLYDLSDAELSALMAELNQPAFRARQIAEWLYKHKVTTFEAMTNLPKALREKLAARARIGGFEQVAEQHSTESATTKRLYRLPDGQLIESVLMEYEDGRRTACISTQAGCAMGCIFCATGQMGFARHLSAGEIVEQALHFARLLEQEGDRLSNVVLMGMGEPLHNYEQTLRAIRRLNDPNGSNIGQRHITLSTVGLVPEIRRFADEGLQVGLAISLHAATDAERSALLPINRRYPIREVLEAARYYVEKTGRRVTFEWALIRGENDTPEQAERLGALLRGLLCHVNLIPLNPTGGYGGAPSDPARVDAFRAVLARYGVSSTVRVRRGIDIQAGCGQLKTEVLRRERTRRAVS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein's S-methylcysteine intermediate must be carefully engineered to ensure that the amino acids involved in adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine are properly positioned for optimal catalysis.2. The designed protein must be able to perform rRNA binding under specific conditions. The designed protein sequence is ",
    "response#1": "STQSGCAMGCAFCATGQMGFRRNLSAGEIVEQVVYFARRLAAHGERVTNIVLMGMGEPLHNYDATLEAVRRLNHPEGMNLGARRFTLSTVGLPDAIRRLAREDLQVNLAVSLHAADDELRAEMLPINRRYPIAQVMEAVRDYVAHTHRRVTFEWALIRELNDHPERAQGEMEVQSVLERGGEPAYRLRQAYAALTGSLVRDWEEATSLPQGLRGALNQEAPAAVLDLRRTWRATDGTRKYLFHTHDGHAIETVMIPEKGRHTVCISTQVGCAMACKFCATGLLGIKRNLGAREIAEQVFVCARDISPERVSNVVVMGMGEPMLNYKATLGALKVLNDPKGFGLAARHIAVSTSGLVDKIRRFADEPEQFHLAISLHTPFEEERREIMPVAARHTIPELMDAARYFVSRTRRKLFFEYTLLAGVNDQPRHVEALAELFDHPLYHLNLLRFNWTDTGFSATSKADAKRFLNHARDLGLSATLRPSRGQDIDAACGQLAARDLRKP",
    "response#2": "LTLCISSQAGCAMACTFCATGWAGYRRQLSAGEIVAQFRGARRWAQENGYDDITNIVFMGMGEPLMNPKAVFPTLAILNRGYKFGARRITISTVGVVPGIQRLAEMPEQYRLAVSLHAPNHELRQKLIPLEKKYDIDELLDALRRFDAAGGKRITFEYVMIDGVTDLPELADELARVVREFNAFVNLIPFNPIPGTEWKPSRRARLDLFVRP",
    "response#3": "ISTQVGCAMACKFCATGLLGIKRNLGAREIAEQVFVCARDISPERVSNVVVMGMGEPMLNYKATLGALKVLNDPKGFGLAARHIAVSTSGLVDKIRRFADEPEQFHLAISLHTPFEEERREIMPVAARHTIPELMDAARYFVSRTRRKLFFEYTLLAGVNDQTPYARVADVEVQSVLERGGEPAYRLRQAYAALTGSLVRDWEEATSLPQGLRGALNQEAPAAVLDLRRTWRATDGTRKYLFHTHDGHAIETVMIPEKGRHTVCISTQVGCAMACKFCATGLLGIKRNLGAREIAEQVFVCARDISPERVSNVVVMGMGEPMLNYKATLGALKVLNDPKGFGLAARHIAVSTSGLVDKIRRFADEPEQFHLAISLHTPFEEERREIMPVAARHTIPELMDAARYFVSRTRRKLFFEYTLLAGVNDQPRHVEALAELFDHPLYHLNLLRFNWTDTGFSATSKADAKRFLNHARDLGLSATLRPSRGQDIDAACGQLAARDEVQSVLERGGEPAYRLRQAYAALTGSLVRDWEEATSLPQGLRGALNQEAPAAVLDLRRTWRATDGTRKYLFHTHDGHAIETVMIPEKGRHTVCISTQVGCAMACKFCATGLLGIKRNLGAREIAEQVFVCARDISPERVSNVVVMGMGEPMLNYKATLGALKVLNDPKGFGLAARHIAVSTSGLVDKIRRFADEPEQFHLAISLHTPFEEERREIMPVAARHTIPELMDAARYFVSRTRRKLFFEYTLLAGVNDQPRHVEALAELFDHPLYHLNLLRFNWTDTGFSATSKADAKRFLNHARDLGLSATLRPSRGQDIDAACGQLAARD"
  },
  {
    "reference": "MPRRLEERRIVLASHNAGKVRENAALLAEYGIAVVSAKELGLPEPAETETTFLGNATIKALAAARAANMVAMADDSGFSIASLNGDPGVYTADWAEQPGGGRDYAKAMAKAEALARPHPDRTAWFTCALVLAWPDGHTEGFEGKAMGEWVWPPRGAAGFGYDPMFQPKGHAETFAEMDPAVKHRISHRAEAFRLLAEGCLKK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.2. The designed protein must be optimized for dITP diphosphatase activity, nucleotide binding. The designed protein sequence is ",
    "response#1": "GFTTD",
    "response#2": "EITQL",
    "response#3": "GVAG"
  },
  {
    "reference": "MLDGKTILLGVTGGIAAYKAAVLCSKLVQKGAQVHVIMTESAARFITPLTLQTLSRNPVHTDTFNEEDPAVVAHINLADRADLVLIAPATANSLAKLAAGLADDMLSTTLLATTAPIIAAPAMNVHMYEHPATVANMETLGRRGVRFIDPGTGQLACGYVAKGRLAEPEEIVQAVELYLASRSMLLGKEVVVTAGGTVERFDPVRYLTNDSSGKMGFAIAEAARDLGAQVTLIAARTTAPTPGGVELVQVESAQQMLDAVLSRADSADVVVKAAAVADYRPIIRHETKLKKTGDTLIIEMEKTTDILAELGRRKNGQFLVGFAAETDRLEEHAMDKLQRKNCDLIVANDVTMEGAGFNVDTNAVSIFDRDGLVEALPVLGKRETADRLMRLVADRLAGSAKAGVKL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a low Km value for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.2. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domain.3. The protein design should consider the endogenous proteins and enzymes present in the coenzyme A biosynthesis, Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway to avoid interference with native metabolic processes.4. The protein should have a high affinity for CTP.5. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.6. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.7. The designed protein should have metal ion binding, phosphopantothenoylcysteine decarboxylase activity, FMN binding to facilitate pantothenate catabolic process. The designed protein sequence is ",
    "response#1": "MNIVLGITAGIAAYKTPQLVRLLTKNGHTVKVILTENAKEFVTPLTLSTVSKNTVFSAFTSVENDWHSHVELALWADVMLIAPATANTIAKMAHGICDNLLLATYFSAKAPVFVAPAMDLDMYAHPTVTENLKQLQSFGNHIIPAAYGELASGLIGQGRMAEPEDIVTFLESYFIRNGKLSGKTALVTAGPTYEPIDPVRFLGNYSSGRQGYAIAEELHDAGAQVTLISGPVSLLPPQNIRLISVQTAQEMLDACLSNIPVDIAVMTAAVADWRVKNISNKKIKKTGDAQTPTFDLITNPDILATIAQKNINRPKLVIGFAAETNDLLDNADAKRKRKNCDWIVVNDVSPDTKIMGGVENEVTIIDSEGIEALPSMSKRDAACRIISHIVDKIK",
    "response#2": "MKILLGISGGIAAYKTPELVRLLKKAGHQVRVACTRNALELVSRIALETVSGEPVLFETFSPRTHPGVEHIDLAGWPDVVVIAPATANVIAKLAGGLADDFLTTVLLATPAPVWIAPGMNTHMWEHPATLENVRRLTGRGVRVIGPDSGELACGTSGPGRMSEPSVIVREAAGRIVGEDEGRVVVGVTAGIAAYKTAILVRLLIKKNLDVTVIPTSASLEMVGKTTWEALTGKPVYTQTPEDAPHVTHVRLGTEADLVVVAPATANTLAKMRAGMADNLLTNTLLVAECPILMVPAMHTQMWENPATVENVQILRARGVEVMEPAAGRLTGKDSGKGRMPEPEEIAARALRLARDLLDGAGDGGLAGRHVVVSAGGTREYLDPVRYLGNRSSGRQGYALARTAALRGASVTLVAANVSLPDPAGVKVTRVGSTRELRDAVLAAARDADAVVMAAAPADFRPAGYADAKIKKADDGSAPTVELTQNPDILAELSHDRPRPGLVVAGFAAETGDATAGVLDHGRAKLARKGCDLLVVNEVGVDKGFESPDNEAVILGDDGRVRALAGRRVLITAGATREHLDPVRFISNPSTGRMGVALARAARELGAEVTLVHGHLEVPVPDGVVAVPAVSAMELHRAVFEWKDRADLLIMTAAVGDYMSASPAAEKMKKEALGATLTLQLVRTPDILAELSQPGNAPSGPGNRPGERRPILVGFAAETAGGDESRLLELARQKLSRKGCDALFANDVSLTGAGFAAETNQGVEVLGPGG",
    "response#3": "MNIVLGITAGIAAYKTPQLVRLLTKNGHTVKVILTENAKEFVTPLTLSTVSKNTVFSAFTSVENDWHSHVELALWADVMLIAPATANTIAKMAHGICDNLLLATYFSAKAPVFVAPAMDLDMYAHPTVTENLKQLQSFGNHIIPAAYGELASGLIGQGRMAEPEDIVTFLRSMLDAGDLWLAGRRVLITAGATREHLDPVRFISNPSTGRMGVALARAARELGAEVTLVHGHLEVPVPDGVVAVPAVSAMELHRAVFEWKDRADLLIMTAAVGDYMSASPAAEKMKKEALGATLTLQLVRTPDILAELSQPGNAPSGPGNRPGERRPILVGFAAETAGGDESRLLELARQKLSRKGCDALFANDVSLTGAGFAAETNQGVELAWQDGRCQWPLPLAGRKVLMTAGPTFEPIDAVRGITNISSGKMGYALARACRNAGAEVTLVSGPTGLPCPEGVQRLDVGTAREMYDAVLRHAGSADIFIGVAAVADYRVRNRAEHKLKKGDSVPVIELEENPDILATVASLPAAPFCVGFAAESRNLLEYADAKRRRKKVPLLVANLVQKAMGRDTNEVLLLAGRHVVVSAGGTREYLDPVRYLGNRSSGRQGYALARTAALRGASVTLVAANVSLPDPAGVKVTRVGSTRELRDAVLAAARDADAVVMAAAPADFRPAGYADAKIKKADDGSAPTVELTQNPDILAELSHDRPRPGLVVAGFAAETGDATAGVLDHGRAKLARKGCDLLVVNEVGVDKGFESPDNEAVILFRHGRTELAGRHVVVSAGGTREYLDPVRYLGNRSSGRQGYALARTAALRGASVTLVAANVSLPDPAGVKVTRVGSTRELRDAVLAAARDADAVVMAAAPADFRPAGYADAKIKKADDGSAPTVELTQNPDILAELSHDRPRPGLVVAGFAAETGDATAGVLDHGRAKLARKGCDLLVVNEVGVDKGFESPDNEAVI"
  },
  {
    "reference": "MSDELLTSVEMGEADRMAIASGPVAGIGLMRRAGEAVAAEVFNRYPEATHVHVLCGPGNNGGDGYVVARILADSGVATTIWASAAPRPESDAALAAAECPIAPRPLSGFEAGPGSIVVDALYGAGLSKPLSGDAARAADIASNRRLPVIAVDLPSGVSGDSGKVLGIAFTAAVTVTFARKKPGHLLLPGRERCGEIVLADIGIGDDIIARLRSRAFENRPARWLRDFPIPAVDAHKYKRGHVGVFSGGPSATGAARLSALAAARSGAGAVTVLSPGNAMQVNAAHLTSIMLRKADDLADIEEFAGERRPSAFVLGPGFGVGEKTRTFGLALLAAGQPRNAAAQVDGLVFDADAITSFREAQDVLFEAARRQNAPALVMTPHEGEFARLFPDMAGDDASSKLDKARAAAARANAIIVYKGADTVIAAPDGRAAINANGAAWLATAGSGDVLSGISAGLMAQAMPAFEAACAAVWIHAEAGSRFGPGLIAEDLPLALVPVLRELVEARRKVPNG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The desired protein should be able to perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate2. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.3. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..4. The protein contains novel YjeF N-terminal domains that confer a unique function or activity.5. The protein design must be able to perform NADHX epimerase activity, ATP binding, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, metal ion binding in order to contribute to nicotinamide nucleotide metabolic process.6. The designed protein should have a high specificity for AMP, (6S)-NADPHX, K(+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MACCDAEAMALGFPEEALMENAACAAIACLRRRIPSCASGLRGMRVLLLAGGGNNGGDAFCMARHFYDAGALPAVVLSRPPAPGGAAARWLDVLRRMPVPIVMAGEWLGGTAQVLDGMRPDLIVDGLMGTGLRGQLRETECRIIGKALEWKRLGAHVFAIDIPSGMTGMSGRPSPIALAADVTAAFHAPKPGLLMPDAAAFTGILEVCSIGMPRAAADAHKGDSGHVMVLGGDSGMAGAALLCARAALRAGAGLVSVGTRAAHAAALVAAQPECMVRAVEAASELDPLLERATVIALGPGLGQGDWGHALFERVLVAHRPCVLDADALNLLAQAPRACAQAVLTPHTGEAARLLGVSTTEVQRDRYTALAHLVQRYQCPVVLKGAGSLVGAPGELPRVIDAGNPGMAVGGMGDLLAGVIAALIAQGLEPFPDARKGDRGRLLIVGGGPYHGAPILAGDAAARSGCDLVHVAMPSXARSRAEWPPHLIPESIPDEVSLTLDSVEHLLGRVLSGRGVQAMVIGPGLGRGEGVAKAVERLIEVAVEESVPVVIDADAISMIPKGRWPAGMVGIITPHSREAEEWIGPEPATIREELXQSVNPEARVVLLTGPVDRLFGPQGRGALGTGGXPRMAVGGTGDLLAGICGGLLAQGMTPWPAARLASALLREAGNVAALERGPGLVASDL",
    "response#2": "MHPAEVEVFDANARHLDISIQDLMQAAGQALAAETIRIVEASEAPEHREIWILCGSGNNGGDGFAAALSLHESGYTVRLIASHVTQKGEVAESFRNACREASLPIDVWPMVPTELKPRAIVDALLGAGAGGSTPRGDVSDLLEWARPYSTNVHVIAXDIPTGFGTNAVLSATSTVTFHAEKIGMRDAAXMVXSTIGHLRVAPLPWPEEVLPSSHKGTYGHVLALGGSPGFTGAITLTARAALRAGAGLVTAAVPRGCQPVVAAGAPEIMTWGLPETAGGSLGRSALDPLLERLENFSVLAIGPGLGRDPETVELVKSLLPRASIPLVVDADALNALATDTGILSQPHGPMVLTPHPGEMGRLIGCPAAKIQEDRLNIARRYAQEWRCVLLLKGARTVVACPDGRAYINPTGNPGMATGGSGDVLTGIIAGLIAQGMSLDKAAAIGAYLHGAAGDLAAEELGQEALIAGDLALGGSPGFTGAITLTARAALRAGAGLVTAAVPRGCQPVVAAGAPEIMTWGLPETAGGSLGRSALDPLLERLENFSVLAIGPGLGRDPETVELVKSLLPRASIPLVVDADALNALATDTGILSQPHGPMVLTPHPGEMGRLIGCPAAKIQEDRLNIARRYAQEWRCVLLLKGARTVVACPDGRAYINPTGNPGMATGGSGDVLTGIIAGLIAQGMSLDKAAAIGAYLHGAAGDLAAEELGQEALIAGDILPDARKGDRGRLLIVGGGPYHGAPILAGDAAARSGCDLVHVAMPSXARSRAEWPPHLIPESIPDEVSLTLDSVEHLLGRVLSGRGVQAMVIGPGLGRGEGVAKAVERLIEVAVEESVPVVIDADAISMIPKGRWPAGMVGIITPHSREAEEWIGPEPATIREELXQSVNPEARVVLLTGPVDRLFGPQGRGALGTGGXPRMAVGGTGDLLAGICGGLLAQGMTPWPAARLASALLREAGNVAALERGPGLVASD",
    "response#3": "MTAIERASEKAGVSTDVLMENAGLAVARQARALVGAAGVQVLVLVGPGNNGADGLVAARHLRRWGAQVTCYVVAGRPDPDAKKDRALEYGVRIIDAAEIGALERLLARSALVIDAVLGTGRARPLAGQVKETMISLAKARSSPTSPKLLALDLPTGLNADTGEVDPFGVPCDLTLALGYPKAGLLNFPGTGHAGELRVLDIGVPPGLPTDDDVADLLPRPEAESDKYRRGVLGIVAGSDRFTGAAVLAVGGAIRAGAGLVRFASAETAVRQVRNRWPEAITTVLDGPSVAEVGRVQAWVLGPGMGTGEEAVALARAVLATDVPVLVDADGLTILANDRSLLRRSAPTVLTPHAGELSRLLDVPRERIEAARMEHVRRAVAELGAIVLLKGSTTLIAEQDRPVRVNTTGTPWLATGGTGDVLSGIAGTLLSGGLSGYDAVSCAAHLHGLAAQAASTGISGGHGPIAAMDVATMLPQVIPDARKGDRGRLLIVGGGPYHGAPILAGDAAARSGCDLVHVAMPSXARSRAEWPPHLIPESIPDEVSLTLDSVEHLLGRVLSGRGVQAMVIGPGLGRGEGVAKAVERLIEVAVEESVPVVIDADAISMIPKGRWPAGMVGIITPHSREAEEWIGPEPATIREELXQSVNPEARVVLLTGPVDRLFGPQGRGALGTGGXPRMAVGGTGDLLAGICGGLLAQGMTPWPAARLASALLREAGNVAALERGPGLVASDAVFSGGSSATGAARLSALAAARTGAGAVTVLSPGNAMQVNAAHLTSIMLRKADTIEDIAEYLAERQPSAFVLGPGFGIGERARAFALRLLQQNRVYDYDRGIELWGIVLDADGLTSFRDNPDTLFEASHRALAPPLVFTPHEGEFARLFPDIARSKKQSKLAKARAAAQRANAVIVYKGADTVIAAPDGHAAINANGAPWLATAGSGDVLAGIIAGLLAQGMPPFKAACAGVWIHAEAGRR"
  },
  {
    "reference": "MGYRVAVVGATGNVGREMLNILHERQFPVTEVIALASKRSVGREVAFGDKKLKVKALDDFNFTGTDFALFSAGGSVAAKFAPIAAAQGCIVIDNSSHFRMDPDVPLVVPEVNAAAAAGYTKKNIIANPNCSTAQLVVALKPLHDVATIKRVVVSTYQSTSGAGNEAMDELFTQTKGIYVNDTPTPEKFTKQIAFNVIPHIDVFMDDRYTKEEWKMMVETKKIVDPAIKLTATCVRVPTFVGHAESVNIEFERELSADRAREILRESPGIMVVDNPEENGYITPVECVGDYATFISRVRDDATIENGINLWCVSDNLRKGAALNTVQIAELVGREYLKKAA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be able to modulate 'de novo' L-methionine biosynthetic process in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "GEVYLVGGGPGDPDLLTFKALRLMQQADIVLYDRLVSPQVLDKVRRDAELVYVGKRAGNHAVSQDNINLKLVEYAQQGLRVLRLKGGDPFIFGRGGEEIATLAEYNIPFQVVPGITAASGCASYAGIPLTHRDHAQSVRFIAGHLMSGELNLDWAELAKPAQTLVFYMGLSGMETICSQLMAHGAPGGRAAAIIEKGTQPDQRNFVADLATLPALVRAAQAGAPTLLIVGEVVRLY",
    "response#2": "GEVYLVGGGPGDPDLLTFKALRLMQQADIVLYDRLVSPQVLDKVRRDAELVYVGKRAGNHAVSQDNINLKLVEYAQQGLRVLRLKGGDPFIFGRGGEEIATLAEYNIPFQVVPGITAASGCASYAGIPLTHRDHAQSVRFIAGHLMSGELNLDWAELAKPAQTLVFYMGLSGMETICSQLMAHGAPGGRAAAIIEKGTQPDQRNFVADLATLPALVRAAQAGAPTLLIVGEVVRLRP",
    "response#3": "VGGGPGDPDLLTFKALRLMQQADIVLYDRLVSPQVLDKVRRDAELVYVGKRAGNHAVSQDNINLKLVEYAQQGLRVLRLKGGDPFIFGRGGEEIATLAEYNIPFQVVPGITAASGCASYAGIPLTHRDHAQSVRFIAGHLMSGELNLDWAELAKPAQTLVFYMGLSGMETICSQLMAHGAPGGRAAAIIEKGTQPDQRNFVADLATLPALVRAAQAGAPTLLIVGEVL"
  },
  {
    "reference": "MKVFITGASGFIGSAVLSELISSGHEVVGLARSDEAAAKIKSIDPAAKILRGDLKDLEILKKGATESDGVIHLGFVHDFKNFEQCCEIDRQATVAMLESLKGSNKPFLYTNGTLSLRPNKVANEQDGIDEDSKILRAVTEQVALSYKDKGVSARIVRLPFSVHGKGDKAFVPILMNIAKAAGKSGYVGQGTNAWAAVHRLDTAPLFRLVLEKGKTGQVYHCVGEQGIPFKDIARVIGEILNVPVASIPVDDAESHFGFLTCFVTRDGPVSSEGTRKELGWQPQQIGLLEDIRANYSLN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein can be expressed in cytoplasm and perform aldehyde dehydrogenase (NAD+) activity without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "PYYAHKGTRGHVLVIGGSYGKTGAVHLAARAALTSGCGLVTALVPQCGYVPLQASFPEAMVLTNGETHLQEIAFALKPAAIAIGPGMGTDEETQQALHNFLFDNKIPMVVDADALNILSENLQWLELLPPKSVITPHPKELERLMGTWNDDFDKIDKLKAFSKKHSVIIVAKDARTLVVNGDAVYVNSTGNAALATGGSGDVLAGLITGLIAQQYTPADAAVFGVYLHGLSADIGVAETGTQAFTASSILHYLGRR",
    "response#2": "QSDKKVQCYEPATMKYLGYFPALSPTEVEERVTLSRKAQKTWAQSSFKLRRQFLRILLKYIIEHQELICEVSSRDTGKTMVDASLGEIMTTCEKITWLLSEGERWLKPESRSSGRAMLHKVSRVEFHPLGVIGAIVPWNYPFHNIFNPMLAAVFSGNGIVIKVSEHASWSGCFYFRIIQAALAAVGAPENLVDVITGFAETGEALVSSVDKMIFVGSTAVGKMIMRNAAETLTPVTLELGGKDAFIICEDADVSHVAQVAVRGTLQSSGQNCAGAERFYVHKDIYTAFIGQVTKIVKSVSAGPPLTGRYDMGAICLQEHSEHLQSLVNDALDKGAEIAVRGSFGHLGEDAVDQYFPPTVLINVNHNMKIMKEEAFGPIMPIMQFSTDEEVIKLANDSRYALGCAVFSGSKHRAKQIASQIQCGVAAINDFASNYMCQSLPFGGVKDSGFGRFAGIEGLRACCLVKSC",
    "response#3": "PYYAHKGTRGHVLVIGGSYGKTGAVHLAARAALTSGCGLVTALVPQCGYVPLQASFPEAMVLTNGETHLQEIAFALKPAAIAIGPGMGTDEETQQALHNFLFDNKIPMVVDADALNILSENLQWLELLPPKSVITPHPKELERLMGTWNDDFDKIDKLKAFSKKHSVIIVAKDARTLVVNGDAVYVNSTGNAALATGGSGDVLAGLITGLIAQQYTPADAAVFGVYLHGLSADIGVAETGTQAFTASSILHYLGRH"
  },
  {
    "reference": "MKLVTANQMRAIEQSCVDAGISLDVLMENAGLAVAEAIRAGFDEYDGGGIFGKRIVILVGPGNNGADGFVTGRHLSEWGVSVTAILCAKRKSPDPKRELAEKAGISVEDGLSADGIGIFQQKLETSDLLIDAVLGTGTARPITEPLSSLLFSALESGVPVVALDLPTGINSDTGGFDIAGLPADQTLQLGYPKLGAALSGNPSLTGEIEVLDIGIPFGLDSQVNTELLTDDLAALINPERPFDSHKGTFGSTLVIGGSQDYLGAVTMAADAASRSGVGLTFVATPEPAYKRVAGNVPEAMYRSLPVSSNGNVDPSAAAQIALEIATRSTSVVVGPGMGNNADTAEFLAILLSQIDLETPLVLDADALNILAKSHKWWERLENPAVLTPHPGEMSRLVGISTAEVQRDRITIALDTAKKFNKIVVLKGAATLIASPDGRLNVSPWVNSGLAKGGSGDVLAGLLGGLLAQQPGEPFEMASLAVHIHGYAAESAKRGVGETGMRATDVIARLGHFYRDQIQRLSN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.2. The designed protein must have at least one YjeF N-terminal domain.3. The designed protein should be optimized for metal ion binding, NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, ATP binding activity in order to improve nicotinamide nucleotide metabolic process.4. The protein should have a fast turnover rate for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.5. The designed protein should be able to compete with endogenous proteins or inhibitors for (6S)-NADPHX, K(+), AMP binding.6. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration The designed protein sequence is ",
    "response#1": "MVAIERQADASGLTYDLMMENAGRNLADAIAEEYPDSRDAGVLGLVGSGNNGGDTLVALARLAQQGWRASACIVRQRSPDDPLVLRLTQAGGNVFMLSPENGFALLEEQIQKHAILLDGVLGTGARLPLKPELAQVLGFVQTRVLENKDIRVVAVDCPSGVDCDSGEHAPESIPAHLTVTMAAVKRGLLQFPAYNLVGKLKVVGIGLGEEIPDRQWVASTLPRRPLDAHKGTFGTALIVAGSVNYTGAAWLAGRAAYKSGAGLVTMAVPQSLHTTLAGQFPEATWLLLPDELGVIAGDAWRVLLAGMERATAILIGPGFGLEETTRAFLANLFAAGAHTRRAGIGLVPSVKTAETQPSTKLPPVVLDADGLKLTARLPNWHKQVPAPAVLTPHPGEMSVLTGLTKETIQRERVKIAEQYARDWGHVVVLKGAFTVIAAPDGQTAVIAVATPALARAGTGDVLAGLIVGLRAQGLEAFPAAVAGAWIHAQAGLAAQEHLGSSASVLAGDVLSGVDAVMAGDDKYSQGVVGVVAGSATYPGAGVLCTGAALRTRPGLVRYAGSAAEGVRAAWPEAIVTDGAPAEAGRVQAWVIGPGAGTDDAARDALVQVLATDLPVLVDADALTLLAAEPELVRRRAAPTVLTPHDREFERFGEPVGPDRIGAARRLAADLGAVVLLKGDATVVADPGGTAYVNATGTPWLATAGTGDVLSGIAGAVLATQGTTGLDTAVAAAVAAHLHGRAGQLAARRGPLVAGDLIRHLAGDDKYSQGVVGVVAGSATYPGAGVLCTGAALRTRPGLVRYAGSAAEGVRAAWPEAIVTDGAPAEAGRVQAWVIGPGAGTDDAARDALVQVLATDLPVLVDADALTLLAAEPELVRRRAAPTVLTPHDREFERFGEPVGPDRIGAARRLAADLGAVVLLKGDATVVADPGGTAYVNATGTPWLATAGTGDVLSGIAGAVLATQGTTGLDTAVAAAVAAHLHGRAGQLAARRGPLVAGDLIRHL",
    "response#2": "MATVPEGTLMRRAATGLSVVCAGLLPGVYGSRVVLLVGSGDNGGDALYAGARLARRGARVEAILAGSRTHAGGLAALLDAGGRTAGTDVIERADLIVDGLVGIGGAGELREPYRTLALAAGAAPGLVVAVDVPSGVDASTGRVDGEAVRAQLTVTFGACKNGLLVDPGRSFCGPVELVDIGTDDDVADLLPRPEAESDKYRRGVLGIVAGSDRFTGAAVLAVGGAIRAGAGLVRFASAETAVRQVRNRWPEAITTVLDGPSVAEVGRVQAWVLGPGMGTGEEAVALARAVLATDVPVLVDADGLTILANDRSLLRRSAPTVLTPHAGELSRLLDVPRERIEAARMEHVRRAVAELGAIVLLKGSTTLIAEQDRPVRVNTTGTPWLATGGTGDVLSGIAGTLLSGGLSGYDAVSCAAHLHGLAAQAASTGISGGHGPIAAMDVATMLPQVIRSTDDDVADLLPRPEAESDKYRRGVLGIVAGSDRFTGAAVLAVGGAIRAGAGLVRFASAETAVRQVRNRWPEAITTVLDGPSVAEVGRVQAWVLGPGMGTGEEAVALARAVLATDVPVLVDADGLTILANDRSLLRRSAPTVLTPHAGELSRLLDVPRERIEAARMEHVRRAVAELGAIVLLKGSTTLIAEQDRPVRVNTTGTPWLATGGTGDVLSGIAGTLLSGGLSGYDAVSCAAHLHGLAAQAASTGISGGHGPIAAMDVATMLPQVIRSLAEIPRPIAAVPGPDSHKYTRGLVTIIAGDMPGASALAASAAMRSGAGVVRLQAREFVPGVPAAVIQMPGDPLDRLDDKRIGALLLGPGLRSGRELLDAALASGHPLVLDAGALAMLADGGPERIEGAILTPHDGEFQKLFGALEGSKVDRARASAAQSGAVIAYKGPDTVVAAPDGRAAIHGTAPPWLGTAGSGDVLAGIIAAERAAGKEPFEAACAGVWRHAEAARRAGPKMIADDLI",
    "response#3": "MVAIERQADASGLTYDLMMENAGRNLADAIAEEYPDSRDAGVLGLVGSGNNGGDTLVALARLAQQGWRASACIVRQRSPDDPLVLRLTQAGGNVFMLSPENGFALLEEQIQKHAILLDGVLGTGARLPLKPELAQVLGFVQTRVLENKDIRVVAVDCPSGVDCDSGEHAPESIPAHLTVTMAAVKRGLLQFPAYNLVGKLKVVGIGLGEEIPEPPDVGARWPVPAATDDKYTQGVVGVLAGSAQYPGAAILCSGAAVAATSGMVRYVGSAAADVVTHYPEVVAATGMKEAGRVQSWVVGPGFGTDTSSRDVLSQVLTSDLPVVVDADALTILSSSPELVRGRTAPTLLTPHAGEFERLTGAAPGSDRVSAVRELASTWGVSVLLKGRATVIAGPDGAVVVSDAGGSAASTAGSGDVLAGILGALLASGLDTVTAAACAARVHALAAATAAHGRGGEFAAPISASPLLGAVSDAIRTVRDDDVADLLPRPEAESDKYRRGVLGIVAGSDRFTGAAVLAVGGAIRAGAGLVRFASAETAVRQVRNRWPEAITTVLDGPSVAEVGRVQAWVLGPGMGTGEEAVALARAVLATDVPVLVDADGLTILANDRSLLRRSAPTVLTPHAGELSRLLDVPRERIEAARMEHVRRAVAELGAIVLLKGSTTLIAEQDRPVRVNTTGTPWLATGGTGDVLSGIAGTLLSGGLSGYDAVSCAAHLHGLAAQAASTGISGGHGPIAAMDVATMLPQVISDKYSGGVVALAAGSDTYPGAGVLCATAAVRATPSMVRFIGDNTITSLLPELVCHPDVPSSGRAQAWVVGPGRGTDSAAATELRKVLAQGLPTVIDADALTLLATNTSLRQLVAAHPLTILTPHEGEFTRLYEAAFSAAPSAAWGDALHRLAEELNSIILLKGRLTRVTAPGQPIYSCNAGHSFAATPGSGDVLSGILGAVIA"
  },
  {
    "reference": "MDRAEKREFVAWLNGAFKESGSVVVAHYTGLTVAQMSDLRSKMRDAGGSVKVAKNRLAKIALQGTESEGIADLFTGQTVVAYANDPITAPKVAVEFAKANDKLVILGGAMGATTLNADGVKSLASLPSLDELRAKLVGMIQTPAQRLAVLTSAPAGQIARVIGAHARKNEAA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have large ribosomal subunit rRNA binding within large ribosomal subunit to regulate translation.2. The protein must exhibit the following characteristics: Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.. The designed protein sequence is ",
    "response#1": "PRKGNVQKREVLPDPIYNSKLVTRLINHMMMDGKRGTSTKIIYGAFDIIKNETGNDPVETFEEAMKNIMPVLEVKARRVGGSNYQVPIEVRPDRRTTLGLRWLVQYSRLRGEHTMVERLAREIMDAANNTGAAVKKREDTHRMADANR",
    "response#2": "PRKGNVQKREVLPDPIYNSKLVTRLINHMMMDGKRGTSTKIIYGAFDIIKNETGNDPVETFEEAMKNIMPVLEVKARRVGGSNYQVPIEVRPDRRTTLGLRWLVQYSRLRGEHTMVERLAREIMDAANNTGAAVKKREDTHRMADANR",
    "response#3": "PRKGNVQKREVLPDPIYNSKLVTRLINHMMMDGKRGTSTKIIYGAFDIIKNETGNDPVETFEEAMKNIMPVLEVKARRVGGSNYQVPIEVRPDRRTTLGLRWLVQYSRLRGEHTMVERLAREIMDAANNTGAAVKKREDTHRMADANR"
  },
  {
    "reference": "MLLNMPQYTQSQVLVIGDVMLDRYWHGGTSRISPEAPVPVVNVEQVEDRPGGAGNVALNIAALGAGAALVGIVGQDEAGEALKTRLNAANIHTDFQIVTDKPTITKLRVVSRHQQLLRMDFETPYAAADSSEFIAKAEKLVPHMGAVILSDYAKGSLHHVQALIAVANRHQVPVLVDPKGADFSRYQGATLLTPNLHEFETVVGRCDTEQALVDKGLALLDQLQLQALLITRGEHGMTLLRRGMPEMHLPARAREVYDVTGAGDTVISVLAASLAAGEALPQAVGLANLAAGIVVGKLGTATISAPELRRAVKQEQGSERGVLTEEQLLMAIEEARAAGERIVFTNGCFDIIHAGHVGYLEDTRALGDRLIVAINSDESVKRLKGPGRPINPVDRRMAVLAGLEAVDWVTYYDDDTPERLLKLIKPDVLVKGGDYTEDQVVGHEIVKAYGGSVQVLSFFENVSTTAIVKKIQAQEKE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit phosphotransferase activity, alcohol group as acceptor, ATP binding.2. The designed protein should contain one or more Carbohydrate kinase PfkB, Cytidyltransferase-like domains that are essential for its function.3. The active site active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "MTNGCFDILHAGHIAYLEEAKALGDRLIIAINNDESVSRLKGKKRPINSLEERMKVLNGLISTDWIVPFEEDTPINIIENIKPDILVKGGDYNLSEVVGAKIVNEYGGEVKILSYIEGFSSSNIV",
    "response#2": "FTNGCFDILHAGHVTYLEEAAKLGDRLIVALNKDESVTRLKGLGRPIVKYESRALVMSGLGAVDWVVGFDEDTPERLLEEIRPDVLVKGGDV",
    "response#3": "MTNGCFDLIHAGHVHYLGQARSLGDRLIVAINDDESVRRLKGDGRPVMPLAQRMAVVAALGSVDWVIAFSGDTPATLIEAIQPDILVKGGDC"
  },
  {
    "reference": "MTTRLVLATRNAHKVTELRAILADAGVDSELVGADAYPQIPDVKETGVTFAENALLKAHALAEATGLPAIADDSGLCVDVLGGAPGIFSARWAGRHGDDLANLDLLLAQLGDIEHPHRAAHFACAAALALPDGTSRVAEGLLRGTLRHQPAGTGGFGYDPILQPDGEERTCAELSAAEKNAISHRGKAFRALVPMLAELLA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.2. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.3. A protein with metal ion binding, nucleotide binding, ribonucleoside triphosphate phosphatase activity is needed to enhance nucleotide metabolic process in a specific context.4. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "MDRRKTKD",
    "response#2": "MXSTQNYELXK",
    "response#3": "MNNEWRIE"
  },
  {
    "reference": "MHLNTLSPAPGSHKARKRCGRGIGSGIGKTGGRGHKGQKSRSGGSVRPGFEGGQMPLKQRLPKFGFTSRKSLVRAEVRLHELNLITGDVVDIHALKDAGLITRNIVAVKVMLSGEITRPITLRGIAVTKGAQAAIEAAGGKVEE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have structural constituent of ribosome, rRNA binding and be active in cytoplasm, large ribosomal subunit.2. For general function, the protein need meet that Binds to the 23S rRNA. The designed protein sequence is ",
    "response#1": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTV",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#3": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG"
  },
  {
    "reference": "MRLSRFFLPILKETPKEAEIVSHRLMLRAGMMRQEAAGIYAWLPLGLRVLKKIEQIVREEQNRAGAIECLMPTLQLADLWRESGRYDAYGPEMLRITDRHKRELLYGPTNEEMITEIFRAYVRSYKNLPLNLYHIQWKFRDEQRPRFGVMRGREFLMKDAYSFDIDEAAARKSYNRMFVAYLRTFARMGLKAIPMRAETGPIGGDLSHEFIVLAETGESGVFCDSDVLNLPVPGDDVDYDGDLTPIIKQWTSVYAATEDVHDAERFEREVPADKKLNTRGIEVGQIFYFGTKYSDKMKALVAGPDGVDVPVHGGSYGVGVSRLVGAIIEACHDENGIKWPEEVAPFRAAILNLKQGSADTDAACEALYKELSAKGVDVLYDDTDQRAGGKFATADLIGIPWQILVGPKGLAEGKVELKRRADGSRENVTPAEAVARLTT",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in prolyl-tRNA aminoacylation. The designed protein sequence is ",
    "response#1": "PWQVVVCVINPKGDAAVADAAASLLQELRDAGLDAALDDRGLRPGAMFADMELIGIPHRVVVSERGLGAGTYEYRSRRASEAESLDKATLLQRLA",
    "response#2": "PWQVVVCVINPKGDAAVADAAASLLQELRDAGLDAALDDRGLRPGAMFADMELIGIPHRVVVSERGLGAGTYEYRSRRASEAESLDKATLLQRLN",
    "response#3": "PWQVVVCVINPKGDAAVADAAASLLQELRDAGLDAALDDRGLRPGAMFADMELIGIPHRVVVSERGLGAGTYEYRSRRASEAESLDKATLLQRLA"
  },
  {
    "reference": "MKPAFTFAEIREAEKSIIEKEGIPSLILMENAGKNAFDVITTIYPDIAERTIVIVCGKGNNAGDGFVIARHFLINSIPVEIFTLSQPDELKGDALVNYEVLVKLSSELCTIYNVTESEPDILYNFLKRIKGKAVIIDALLGSGIKGSVTGLYEKIINQVNKIKYKNIKIDVISVDVPSGLGEDNSGGAIIDACYTITMGAVKTNLLNGRGKENTGQLFVVPIGITNELIEKYNNYNKYIVEEDDVKRGLPIRKKTSYKYSNGKALVIGGSKGLSGALIMSSLAALRAGSGAVMAAYPGSLSAHFGRKLYEVIKTELPETDEGTISAEAFDKLNKQLAKADAVLIGPGLSLNSSTAEFLFEVISRCEKPMVIDADALTLLAGNLNLLKQISNKNIILTPHIGEFSKLSGLTTDDITADRFLAVRKFAGEYNVNVILKSETSFACTPEGKIYINNTGNEQLASAGSGDILSGIVVSMLSQTKDTLSAMLCGNYILGRIAEKYSGKAGNRQTALQKDMIDLIPGVITEILE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a high affinity for (6S)-NADPHX, K(+).2. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.3. We need a protein that can perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate with high efficiency.4. The designed protein must be able to perform ATP binding, ADP-dependent NAD(P)H-hydrate dehydratase activity, kinase activity under specific conditions.5. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "MKQLDQLATAEYAVPGILLMDNAAQAVADAVNDSLDELDGESVVVFCGGGNNGGDGFGAARWLQNYGAKVRVFVVGKALDAVTGDAAAELAMLQKTDAKVEAIAGEDDWVVAELAAAKADILVDALAGTGFHGELEGDLLRACRLLNDSEKYIVAVDVPTGVNADNGAVAENAVRADKTVTMALLKTGLLLYPGREYCGDIELADIGMPAKMVGQALWAAVFPSLDLESHKYARGHAIVVSGPPARTGAARLGARGALRIGAGLVTVASEPDALAANAAQLTAIMVEPFQGAVGLSDLLADERKNAALIGPGAGVGDETRENVLALLATQAALVLDADALTSFETDSDTLFAAIKARAATVAITPHEGEFQRLFSGAISEGSKLDRSRRAAEMSGAVVILKGADTVVAAPDGRAAINENAPPWLATAGSGDVLAGFITGLLAQHMPAFEAAAAAVWLHGAAAEAFGPGLIAEDLPESLPECLAGLLS",
    "response#2": "MREIDNHTIEVLGIPGIVLMENAARGVCEVIYDRVKGSSALVVCGKGNNGGDGLAVARNLYNMGYDVEVILTAPVEELKGDAKKNAEILSALPVPVHVVDSTEKLFELYTFAKECDFIVDGLFGTGLSKPLSGFYAELVDFINRLNKTVISIDIPSGLSADTGQVIGPHVKADYTVTFAFPKVAHVMPPACYSVGEVFVVDISIPEDVPGQALWAAVFPSLDLESHKYARGHAIVVSGPPARTGAARLGARGALRIGAGLVTVASEPDALAANAAQLTAIMVEPFQGAVGLSDLLADERKNAALIGPGAGVGDETRENVLALLATQAALVLDADALTSFETDSDTLFAAIKARAATVAITPHEGEFQRLFSGAISEGSKLDRSRRAAEMSGAVVILKGADTVVAAPDGRAAINENAPPWLATAGSGDVLAGFITGLLAQHMPAFEAAAAAVWLHGAAAEAFGPGLIAEDLPESLPECLAGLTK",
    "response#3": "MTAEEARALLPPRDPAGHKGTFGRALILGGSEGMTGAAVLAARAAARSGAGLIYSAYPAPLGPIMESHLIEPVKLPLEGDERWFTPRHLEAVLEHAERMDAVALGPGIGTRKPTARFVQAVLENVKAPMVVDADGLNLIAKRRVDLRRRPGPTVLTPHPAEAARLLELETGEVVRRRLDVMTEFAARHNVVVVLKGAQTLTIAPDGQRTVNPTGNSGLAKGGSGDVLTGLIAGLLAQGLSALDAARLGVFLHGLSADVVAERISVRAMLPSDLLDAFAYAHKGDAGRAVICAGSPGFTGAAALSSYAAVKAGAGLVALYTPLSSRDVLAGKLTEVMVHGLLERMPGILGGGATGDVVKNANAADVLAIGPGLGTSESTQQVVRDILLQVETPVVIDADALTALQGHTEILTQMQAAKVLTPHPGEMARLIGKEISEINADRINIAAQYAQEWNAVLVLKGAPTVIACPDGTVYVNSTGSSALATGGSGDVLTGIIAGLAAQEITLQEAAVCGVYLHGKAADAYAHKGDAGRAVICAGSPGFTGAAALSSYAAVKAGAGLVALYTPLSSRDVLAGKLTEVMVHGLLERMPGILGGGATGDVVKNANAADVLAIGPGLGTSESTQQVVRDILLQVETPVVIDADALTALQGHTEILTQMQAAKVLTPHPGEMARLIGKEISEINADRINIAAQYAQEWNAVLVLKGAPTVIACPDGTVYVNSTGSSALATGGSGDVLTGIIAGLAAQEITLQEAAVCGVYLHGKAADYLVAGQYAKMGAAVIASKACLRAGVGLLTVHCPKSGVNVLQIALPEAMVDADADENENSELANLSPKHTVGIGPGIGKSEKVRKMFEQLLHGVSKPIVVDADALNLLSEHRQLISLLPPNSILTPHPTEFDRLAGETFGSGFDRLQSARKIASQWAVIVVLKGAYTAIALPDGRVYFNSTGNPGMATAGSGDCLTGILTGLLAQGYSPEEASLIGVFVHGLAGDLALERAFSVESLLASDI"
  },
  {
    "reference": "MQAKAVTKYVRISPTKVRPVMDLVRGKPVDRALAILRYLPHKAAREIARTIESARANATNNYDMAPDALIVKYIFADEGPAFKRIMPRARGRADRIRKRTTHITVIVDDGEEM",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome..2. A protein that contributes to translation by functioning in large ribosomal subunit with rRNA binding, structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "ML",
    "response#2": "MXXXGXAAX",
    "response#3": "MI"
  },
  {
    "reference": "MGATSQKELQQEFVIVGEPVVPGIGLGKSLLLGKSSLRIRELTLPQEEVEHEINRYYKALKESRSDLAALEKKAKGKQGYQEIASILQAHLEIIKDPLLTEEVVKTIRKDRKNAEFVFSSVMGEIEKSLCAVQKTTAIAVDRIQDIHDISNRVIGHLCCQHKSSLGESDQNLIVFSEELTPSEAANANPKYIRGFVSLEGSKTSHTAIVSLAKNIPYVANFSAESWQRIREYNGNLVLINGEKGEITFNPKLSTIQAYYRKQSAVSMTVPIQIEKTQPLISLSAQIVGVDELGSISREFPGTTIGLFRSEFMAFSLGRLPFVEEQVAEYTKLVQFSCSDINVLRLFDFGEDKVCPFIASAHRSVRWLLEQETILRGQLQAIAIASRQGKLKVLIPGVLDASEIILVKQMFQEEVQLQNGISENIIWGSMIEIPSAVWMIEEILQESSFIALGTNDLAQYTLGMSRERSLPGNWKQVPHPSVIRMIHYVAARAKQRNIPVSVCGEMAGDHLLLPMFIGFGVRELSVVAPAIHSLKMRLLALNSKECSRLAKQLLRARTYEEVHKLLNM",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a high affinity for phosphoenolpyruvate, Mg(2+).2. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.3. The ideal protein would be one that plays a critical role in phosphorylation, phosphoenolpyruvate-dependent sugar phosphotransferase system through phosphoenolpyruvate-protein phosphotransferase activity, metal ion binding, kinase activity within cytoplasm.4. The protein must exhibit the following characteristics: Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).; This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane; General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS).5. The design of the protein must focus on the precise arrangement of amino acids in the Tele-phosphohistidine intermediate, Proton donor to enable effective catalysis of L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-   L-histidyl-[protein] + pyruvate. The designed protein sequence is ",
    "response#1": "LVGISSSPGYALAPVFRFARDEVVFDTDAADAAFETDRLDTALQTAWHELEELHDEVWKTSGPARAAIFRAHQEFLHDPEMVAEAKALIGQGRSAGFAWHRVFSDRADMLGAMKDAVLSGRAIDLRDAGQRVLQHLGRVRTGETHLPTAPCILLADDLTPSDTARLDPALVRGLATAQGGPTSHTSIIARALDIPAVAGVGPRLLDLATGTPVLLDGGAGVIVVAPTEADKARAETAMAALTAQRELEARERYKPALTVDGARVEVVANISDVAEAIASVEAGAEGVGLLRTEFLFVNREAPPGEDEQLAIYAAMLSALNGLPIIIRTLDVGGDKEIPYLRMPVEQNPFLGERGIRFCLSHEDLFRTQLRAIYRASAGGQVRIMFPMIAMIEELETARRIAEEVRLEVGAAPVEIGIMIEIPSAVMMAPELAKRVDFFSIGTNDLTQYALAMDRMHPVLAKQADGLHPAVLRLIDSTVRAAEAARIWVGACGGIGDVFGTTEQQRVWLTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPAAKARMAQL",
    "response#2": "LVGISSSPGYALAPVFRFARDEVVFDTDAADAAFETDRLDTALQTAWHELEELHDEVWKTSGPARAAIFRAHQEFLHDPEMVAEAKALIGQGRSAGFAWHRVFSDRADMLGAMKDAVLSGRAIDLRDAGQRVLQHLGRVRTGETHLPTAPCILLADDLTPSDTARLDPALVRGLATAQGGPTSHTSIIARALDIPAVAGVGPRLLDLATGTPVLLDGGAGVIVVAPTEADKARAETAMAALTAQRELEARERYKPALTVDGARVEVVANISDVAEAIASVEAGAEGVGLLRTEFLFVNREAPPGEDEQLAIYAAMLSALNGLPIIIRTLDVGGDKEIPYLRMPVEQNPFLGERGIRFCLSHEDLFRTQLRAIYRASAGGQVRIMFPMIAMIEELETARRIAEEVRLEVGAAPVEIGIMIEIPSAVMMAPELAKRVDFFSIGTNDLTQYALAMDRMHPVLAKQADGLHPAVLRLIDSTVRAAEAARIWVGACGGIGG",
    "response#3": "LSGKRISKGIGIGEVLCIRKNFDKIISREKIDFSQVDSEISKFNKAKSKAIEALRDLERKAMLQFGDDKKGIFEGQVLIVEDDELDELVIELIVKENYSAAYSIYLAFENLVKSVEDYKDPYLKERASDYKDIRNRLISIILGQVSDFSEINKDIILVTEELTPSDTMQFDLNYVKGFLTAVGGETSHAAILARTMGLPALVMTLSDIDALKDGDKIVIDAMSSIVIKNPSSDEINLYEGKILRQVELEKELFSLKDKDAETKDGTKVFLKANIGTPVDITYVNKYGVEGIGLFRTEFLYMRSLQPPTEDEQFETYKRVIETMEKKGVVTIRTLDVGGDKEIPYLNFKKEENPFLGFRALRMYKEYEELIQAQFNAIFRASHYGKIRVMVPMLTIYEEIETIEYFVNNAKINLKSRGLPFDENLEVGCMIEVPSAALISSKLANKLKFFSIGTNDLTQYVLAVDRGNQKISNLYDKYNPAVLKLIKKVLDDGVSSGIDVSVCGELGGDDAGALLLVGLGFRSLSMIPSATLRIKYLLKKYTIMELEELANKVLNSDSKQETLSYFNNYVK"
  },
  {
    "reference": "MTFSGHTARKRFGQHWLINERVLDRIVEAAELQDGDRVLEVGPGRGALTERLLASAAAAIHAVELDRDLVAGLQQTFASHPKFSLQEGDVLSVPLELSGGVPANKVVANIPYNITGPLLDRLIGRLDRPVDFSYQRLVLLVQHEVAQRIRARPGHSNFSALSVRMQLLGRCSHVCPVPPRCFQPPPKVQSEVICIDPFPPERRPTAALSRGVERLLKMAFLSRRKMLRNTLAPVGSTDLLQSLAEEAGISLQQRPQDVAPEAWVALAKGLNQVDSVA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein can be targeted to cytoplasm and perform RNA binding, 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity.2. The designed protein should have a binding site that is specific to the target S-adenosyl-L-methionine ligand.3. For general function, the protein need meet that May play a critical role in biogenesis of 30S subunits.; Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle4. The desired protein should be able to perform adenosine(1518)/adenosine(1519) in 16S rRNA + 4 S-adenosyl-L-   methionine = 4 H(+) + N(6)-dimethyladenosine(1518)/N(6)-   dimethyladenosine(1519) in 16S rRNA + 4 S-adenosyl-L-homocysteine The designed protein sequence is ",
    "response#1": "VIQEIVAAVGPAPSDRLVEIGPGEGVLTRELLQSGACLEAIELDRDLVAALKRRFAGVGRLRIHEGDAMKFDLRTIATGERLRVVGNLPYNISTPLLFHLFDQIDVIEDMHFMLQKEVVDRLCAGAGDDHYGRLSVMAALYCQAQHLFDVGPECFHPQPKVVSAVVRLVPHKSALSTSAGLVAAVHEAIVRAAQLPPPQRDPAWVLEIGPGTGQLTQRLLAQGVHVVAVEIDRDLCRLLQKRFADQPRFHLVEGDFLRLPLPPQPRLLVANIPYNLTGPILEKVLGSPAQPVRQFERIVLMVQKELAERLQAGPGSKAYGALSVRVRYLAECELICRVPPSAFRPPPQVESAVVRLTPRKAPLTVTAGLVAAAKPKPAVLPTAALVAAAKPKPAVLPTAALVAAAKPKPAVLPTAALVAAAKPKPAVLTTAALVAAAKPKPAVLPTAALVAAAKPKPAVLPTAALVAAAKPKPAVLPTAALVAAAKPKPAVLPTAALVAAAKPKPAVLPTAALVAAAKPKPAVLPTAALVAAAKPKPAVLPTAALVAAAKPKPAVLLPAALVAAAKPKPAVLPTAALVAAAKPKPAVLPTAALVAAAKPKPAVLPTAALVAAAKPKPAVLPTAALVAAAKPKPAVLPTAALVAAAKPKPAVLPTAALVAAAKPKPAVLTTAALVAAAKPKPAVLPTAALVAAAKPKPAVLPTAALVAAAKP",
    "response#2": "IAKRIVKEADLKPDDVVVEIGAGAGTLTEELAKTGARVIAYEIDEGLAPILQERLSKYPNVELRFEDFLKARDVPEEAICVSNIPYSVTGPIMEKIIEWRFKKAIVMVQKEVGERILSKPGRKSYGYLSVVVQTFYEVRKLFDVSRSYFVPNPEVDSVVVEMKRKKYPFTRYDSEKNVLEIGPGLGTLTRDMSFYVNEIFAIELGFNLKFVLEKLKDYSMKLVEIGPGKGAITAQLIESCPSMQVVELGFNLKKLIKELFEKNS",
    "response#3": "VVEIGAGAGTLTEELAKTGARVIAYEINDNDDHPNGNYDKLSINELKEKFNFPEDILEKIVEAAELCENDRVLEIGPGRGALTHKLLNSKVSLVHAIELDTDLVVGLRERFFHETRFSLKGGDALKISLLPPDGIEVNKVVANIPYNITSPLLERLIGKLGFFPETRYERLVLLLQKEVADRILAMPGQSSFSAMSVRVQLLCKSRSVCDVSPKCFKPSPKVHSKVVVIEPFSFGSKTDQ"
  },
  {
    "reference": "MTGWIVLPISLTVFSIPGIWIVYAMAVMNHHVCPVENWTYNLTCTDDNTKAGTPKSCCTLEDVPLISKCGTYPPESCLFSLIGNVGAFMVVIICLLRYSQVIEVSQRSWLNTTALIAGCTNAAGLVMVGNFQVDYAKSLHYIGAGVAFPAGLLFVCLSSILSYQIAASALDYWLGHLRVSLTIVALISLVLSGVFFIQESFLMQHLAAICEWIFVLDILVFYGTFAYEFGSVSTDTMMAALQSSSARSCKSPGSSSTSTHLHCNAERIAMI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must be able to regulate phosphatidylinositol phosphate biosynthetic process.2. The designed protein should contain Cytoplasmic topological domains.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.4. The protein must exhibit the following characteristics: Regulates localization of phosphatidylinositol 4-kinase (PI4K) to the plasma membrane.. The designed protein sequence is ",
    "response#1": "MTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKC",
    "response#2": "VSIMATGGGLRSMTSMFGHLLGLQKLNVLNCTSYVTGLSGATWTMAHLYKDAHWSSKNLEKTIQEVRRQAVKNKIPALYSEQLSYYRQDLQERSQEGYKVTLIDLWGLLLESCFFDERYEGKLSWQKEALCQGQNPLPIYLTINVKDNVSNQDFREWVEFTPYEVGFQKYGAFIPSEYFGSEFYMGRLMKRIPESRICYMIGLWSSIFSLNLLDAWNLSHTSEEFFHMWTREKMQDIEDEPILPEIPKCDANALECL",
    "response#3": "VSIMATGGGLRSMTSMFGHLLGLQKLNVLNCTSYVTGLSGATWTMAHLYKDAHWSSKNLEKTIQEVRRQAVKNKIPALYSEQLSYYRQDLQERSQEGYKVTLIDLWGLLLESCFFDERYEGKLSWQKEALCQGQNPLPIYLTINVKDNVSNQDFREWVEFTPYEVGFQKYGAFIPSEYFGSEFYMGRLMKRIPESRICYMIGLWSSIFSLNLLDAWNLSHTSEEFFHMWTREKMQDIEDEPILPEIPKCDANALECL"
  },
  {
    "reference": "MSKAYEQSGVDIHAGYEAVERMSSHVKRTMRKEVLGGLGGFGATFDLSQLNMKAPVLVSGTDGVGTKLKLAIDNDKHDTIGIDAVAMCVNDILTTGAEPLYFLDYIATNKVVPEVIEQIVKGVSDGCEETNTALIGGETAEMGEMYHEGEYDLAGFAVGAVEKDDYIDGSAIQEGQAIIGLASNGIHSNGYSLVRKLIKESGIDLNDDFDGRTYLETFLAPTQLYVKPILALKKAVQIKGMNHITGGGFYENIPRGLPEGLAANIDTQAFPTPNVFNWLQQQANIDTKEMYNVFNMGIGFTVIIDKKDVAQTLDILKQEGISAFEIGEIIEDKDTPIHLEGVE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must have high specificity towards ATP binding.2. Ideal protein should have a binding affinity for key enzymes in the IMP biosynthesis via de novo pathway pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MASVKDRRVESPATDTDLGRGVFEFSDRYSVFDWGEMPDHVPGKGASLCTMGAYTFEQLAAAGVPTHYQGVRTPDGETVRLADAPEAPTQMVIDLTQVPTLPFEDGSYDYDRYHDAGGSNYLVPLEVVFRNAVPVGSSLRTRCAPADVGIDADEWPQGPVELPETIVEFSTKYEEQDRYLSRSMADEIAGDADIAELDALARRVNETITDCAADAGFVHDDGKLECVYVDGEVRVADVAGTFDENRFRFDGREVSKEAVRQFYKRSDPEWVGAVKDAKRAADERGVADWKSLCEPSPDPLPPEILQAAADLYAAGANRYTR",
    "response#2": "MASVKDRRVESPATDTDLGRGVFEFSDRYSVFDWGEMPDHVPGKGASLCTMGAYTFEQLAAAGVPTHYQGVRTPDGETVRLADAPEAPTQMVIDLTQVPTLPFEDGSYDYDRYHDAGGSNYLVPLEVVFRNAVPVGSSLRTRCAPADVGIDADEWPQGPVELPETIVEFSTKYEEQDRYLSRSMADEIAGDADIAELDALARRVNETITDCAADAGFVHDDGKLECVYVDGEVRVADVAGTFDENRFRFDGREVSKEAVRQFYKRSDPEWVGAVKDAKRAADERGVADWKSLCEPSPDPLPPEILQAAADLYAAGANRYTN",
    "response#3": "MASVKDRRVESPATDTDLGRGVFEFSDRYSVFDWGEMPDHVPGKGASLCTMGAYTFEQLAAAGVPTHYQGVRTPDGETVRLADAPEAPTQMVIDLTQVPTLPFEDGSYDYDRYHDAGGSNYLVPLEVVFRNAVPVGSSLRTRCAPADVGIDADEWPQGPVELPETIVEFSTKYEEQDRYLSRSMADEIAGDADIAELDALARRVNETITDCAADAGFVHDDGKLECVYVDGEVRVADVAGTFDENRFRFDGREVSKEAVRQFYKRSDPEWVGAVKDAKRAADERGVADWKSLCEPSPDPLPPEILQAAADLYAAGANRYTN"
  },
  {
    "reference": "MNNLIKNMAIWLVIALVLMTVFNQFSTRQPSQPPMEYSQFISEMHQGRIAKVVIDGRTLRGSKTDGRQFIVHSPSDPWLVSDLLKAGVSVEAKPEEEPSMLMSILVSWFPMLLLIAVWIFFMRQMQGGGRNGGAFSFGKSKARMLDHKNNNVTFADVAGCEEAKEEVAELVEFLRDPSRFQKLGGRIPRGVLMCGSPGTGKTLLARAIAGEAKVPFFSISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRQRGAGLGGGNDEREQTLNQLLVEMDGFEGNMGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQILQVHMRKVPVSPDVKAGILARGTPGMSGADLANLVNEAALFAARAGKRLVDMDDFERAKDKILMGAERRSVVMPENERRNTAYHESGHAVVAYLLPKTDPVHKVSIIPRGRALGVTMQLPSEDRYSMDRDQILQTIAVMFGGRIAEEVFMSQMTTGASNDFERATDTARKMVMQWGMSETLGPMVYGENEGEVFLGRSVTTHKNLSEATMQKVDAEIRRIIDEQYALARKLIEENKDKIEAMTQALLEWETIDSDQIKDIMEGRPPRPPQAPTSSARQDKGSAGSAASEVKQPAGEPATTEAPAAQKVEI",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be involved in cell division, proteolysis, protein catabolic process by localizing to plasma membrane and exhibiting metalloendopeptidase activity, ATP hydrolysis activity, ATP binding, zinc ion binding.2. The presence of AAA+ ATPase domains is critical for the interaction of the designed protein with its intended targets.3. The presence of Zn(2+) is crucial for the protein to function optimally under specific conditions.4. For general function, the protein need meet that Plays a role in the quality control of integral membrane proteins.5. The designed protein should have a high specificity for Zn(2+), ATP binding, with minimal off-target effects.6. The active site active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "MVMTEDEKKLTAYHEAGHAIVGLNVPQHDPIHKATIIPRGRALGLVLSLPERDQLSVTYTKYKSKIAMAMGGKVAEELIFGKENVTSGASSDIQQVSKIARAMVTQFGFSEELGNIDYANEQQSYLGNYQGQTAISPETQQKIDAEVRRIVDEGYERAKQILTDKNDDLHRLAQGLLEYETLTGSEITKKWEGYKFVID",
    "response#2": "MVMSDDEKKMTAYHEAGHALVAMHEPECDPVHKATIIPRGRALGLVMSLPAGDRYSKHKSKLKAELSMAMGGRVAEELIFGADKVSNGASGDIKMATNQARMMVTEWGMSEKLGMIAYSDNSQEVFLGHSVTQSKNVSEATAREIDSEVRSIIDEAYARAKHTLQTNIEELHALAKGLLEYETLSGDEIRTRLDEVKARKAQIPRGVLLYGPPGTGKTLLARAVAGEAGVPFFTISGSDFVEMFVGVGASRVRDLFEQAKQNSPCIIFVDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFGERTGVILIAATNRPDILDPALLRPGRFDRQIPVGAPDVDAAVIARGTPGFSGADLANLVNEAALFAARTNQRVVSMEEFDTLDGIVDASLEILQKKQQTDYTFEYDVSIEAIARRTPGFAGADLANLLNEAAILTARRRKPAISLEEIDA",
    "response#3": "VLLVGPPGTGKTLLAKAVAGEAKVPFFSLSGSDFVEMFVGVGASRVRDLFAQAKAKSPAIIFIDEIDAIGRARGKNNFSGGNDERENTLNQLLTEMDGFGTDTNVIVMAATNRADILDKALMRAGRFDRSIYVLGSGVEELLEVSGRGEMLSRVDLEEIARSTPGFSGADLHNLLNEAALIAARYKRKSIEQQDISRLYGCSIIIVAATNRPDVLDPALLALGRADSLAALELAAGDLGLDSLAGDALSCLKGPDKG"
  },
  {
    "reference": "EGCCSNPPCRHNHPEVC",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have ion channel regulator activity, acetylcholine receptor inhibitor activity and be active in extracellular region, host cell postsynaptic membrane. The designed protein sequence is ",
    "response#1": "PEVLTPQLARVVSDGEVLYMPSIRQRFSCDVSGVDTESGATCRIKIGSWTHHSREISVDPTTENSDDSEYFSQYSRFEILDVTQKKNSVTYSCCPEAYEDVEVC",
    "response#2": "PEVLTPQLARVVSDGEVLYMPSIRQRFSCDVSGVDTESGATCRIKIGSWTHHSREISVDPTTENSDDSEYFSQYSRFEILDVTQKKNSVTYSCCPEAYEDVEVC",
    "response#3": "PEVLTPQLARVVSDGEVLYMPSIRQRFSCDVSGVDTESGATCRIKIGSWTHHSREISVDPTTENSDDSEYFSQYSRFEILDVTQKKNSVTYSCCPEAYEDVEVC"
  },
  {
    "reference": "MPHADQSHTSHQDAPAVTPSVTNPGAPRPTRVRPVPLTELAGQLGIAAPHGAAGVAVTGITHDSRAVRPGDVYAALPGARFHGADFAAQAAGQGAAAVLTDPSGADRAAATGLPVLTVDSPRDRMGALAATIYGEPGRDLLQIGITGTSGKTTTAYLIEGGLRAVGGPTGLIGTVESRIGDERLKSERTTPEATDLQAIFAVMRERGVRSVAMEVSSHALRLGRVDGCVFDVAVFNNLSPEHMEFHTGMEDYFAAKAELFTKERSRVGVVNLDDEYGRRLAEGESEVPVTTFSAEGRPDADWRAVDVEIGPFDSAFTVLGPEGRTITARAPLAGPFNVANALAAIVALTAAGIDPQTAADGVAAVPGVPGRLERVDAGQPYLAVVDYAHKTDAVESVLRALRKTGPGRIHAVLGCGGDRDTTKRAPMGRALARLADTAVLTSDNPRSEDPLAILATMLAGAAEVPAAERGTVLLLEDRAAAIAAAVARAEPGDTVLVLGKGHEQGQDIAGVVRPFDDRQVLREAIEAEQLRSSNKHRNDQG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be able to bind UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate ligand in a variety of conditions2. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.3. The designed protein must be able to regulate cell wall organization, regulation of cell shape. The designed protein sequence is ",
    "response#1": "VTGTNGKTSTAWWLAQALSKLGRRCGVVGTLGVGEPGAMVPTGLTTPDPVLLQQQLRRFVDEGFAACVMEASSIGIAEKRLDAVAVQVAMLTNFTQDHLDYHASMEAYWQAKRELFDWPGLKAAVINVDDAHGDQLAQSLAGRAMDLWTVSVTGPARLQARAVSYASGAMHFDVVEGGNSCSLTAPLVGQYNVSNLLGVIAGRMNRLGGRDAQPLVVVDYAHTPDALEQALQSLRDHAKGRLLCVFGCGGERDAGKRPQMAAIAQRLSDYVFVTDDNPRGEDGERIVADILAGFDGMTTVQVQRERAQAIAAAIAMAGAEDIVLIAGKVR",
    "response#2": "VTGTNGKTTSAFMLERAFWAMGRRTGLIGTVEVHVAGKTLPVAYTTPEAPDLQRLLARMRDASVGAVAMEVSSHGLVLGRVAGTHFSCAVFTNLTRDHLDFHPTMQDYESAKASLFASGLSERGAVNADDAAGRRLLAAGAIPLTSYGTSDDADVRAIDVFASRSGSRVRIPGRYNVSNALGVLAETTVGAILFD",
    "response#3": "ITGTNGKTTTTHLVEFLLNNAQLPTALFGTLYARWPGFAETAAYTTPFPVELQNQLAQAVAAGSKYGVMEVSSHSLDQKRIWGCTYEVAVFTNLTQDHLDYHKDMDDYFEAKALLFSSDYLKGRAIVNADDPYGKKLIARLESEKVWTYSTSDTSADLWASDLEYKPTGVKGILHTPQGEIPFTLPLVGQYNLSNMLAAVGLAIG"
  },
  {
    "reference": "MKNDERTPLPDALAQPLERFYAYLHTEKGLSLYTQRNYKQQLETMTQYLVQVGLTHWTQLDSAWVRQLVMQGKRQGMKASSIATRLSSLRSFLDFLILRGELQANPAKGVSAPRKQRTLPKNLDVDEMAQLLEVTDDDPLSIRDRAIMELMYGAGLRLAELVSIDIKDVNLSEGEIRVIGKGNKERKVWFAGQAQEWVGKWLKLRSQLADSAETALFVSKLGTRISHRSVQKRMAEWGQKQAVASHISPHKLRHSFATHMLESSNNLRAVQELLGHENIATTQIYTHLDFQHLAQVYDQAHPRARKKNKDD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The O-(3'-phospho-DNA)-tyrosine intermediate, active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The protein must exhibit the following characteristics: The XerC- XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division; Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules; It also contributes to the segregational stability of plasmids..3. The protein contains novel Tyr recombinase, Core-binding (CB) domains that confer a unique function or activity.4. A protein that can perform DNA binding in cytoplasm to support cell division, chromosome segregation, transposition, DNA-mediated. The designed protein sequence is ",
    "response#1": "MAESVVGEFLEFLKKNKRLSKNTLESYSRDVEQFLTYMNEHGINFCSAKKSTIVNYLYFLKQQGKSQATISRALSSIKAFYHYLVQFN",
    "response#2": "NMDMEQVQQLLTNESKEPIDLRDKAMMELMYSSGLRLSELQSLNLNSINIRSREVRVIGKGNKERILPFGRYASQAIQQWLKVRLLFNPKDEALFVSQLGNRISHRSIQKRMETWGIRQGLNGHLNPHKLRHSFATHMLENSSDLRAVQELLGHSNLSTTQIYTHLDFQR",
    "response#3": "DCEENFEKSFLFWLAKYVKFKLNSLSNKELKNPQALAEVNFALTKGVKNIDELDALAKKARNAGLSGVNTYFNPLKKVFEYLNFYKLYSLKQIDEELIVEVLASITGALSDASKKNYRIAVINFFDFLDKQR"
  },
  {
    "reference": "MKRIKYVFITGGVLSSLGKGIAAASIGRLLESHGYKITIQKFDPYLNVDPGTMNPFQHGEVYVTEDGAETDLDLGHYERFTSTQTSRAHNWTAGRIYESIIKKERKGEFLGKTVQVIPHVTDEIKAAIHGVDNDNDIVIVEIGGTVGDIESLPFLEAIRQMRLELGLENTLFIHLTLVPFIKTSGELKTKPTQHSVKELRAIGIQPDILLCRTDRFLTPDLKAKISLFTNVPLEAVITAKDVENIYEIPLIFAQEGLDGIILKKLQLTSHKRDLKRWKAFVDRMKKPRGEVNIALVGKYAELHDSYLSLKQALDHGGVANCLKVNIAWIEAEDLEREGSEALLKVHDGILVPGGFGRRGIEGKIRAIAYARENFVPYFGICLGMQLATIEFARHVARLPRANSTEFDPETPDKIFFLWRELKPEHDLGGTMRLGAYACQLKRPSIAYNSYRKSIIYERHRHRYEFNPAYEEILTKAGLVISGRNPDHQLVEIIELPGHPWFLGCQFHPEFKSRPLAPHPLFKSFIGASYAYRLQRNKTF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have CTP synthase activity to facilitate glutamine metabolic process, 'de novo' CTP biosynthetic process.2. The active site, Nucleophile; for glutamine hydrolysis active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP, UTP, Mg(2+) binding.4. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen5. The designed protein have CTP synthase N-terminal domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "VTIAVVGKYVDHKDAYKSVGEALKHGGLRQRTKVNLKWLESQDVEADAGVLLGDVDGILVPGGFGDRGFEGKVLTAQYAREHGVPYFGICYGMQAAVVDYARHVAGLEGANSTENDKQCLHPVIGLITEWRTNTGEIEKRDEKSDLGGTMRLGLQEQRLKPGTLVRELYGKDVVAERHRHRYEFNNRYRTQLEDAGLVIAAKSMDDLLVEMVELPRAVHPWFLACQAHPEFLSTPRDGHPLFVGFAS",
    "response#2": "LPDAYLSVTEALRAGGFAHRARVEIKWVASDECETPAGAAEALDGVDGVLIPGGFGIRGIEGKLGAIKHARTRGIPTLGLCLGLQAMVIEAARNMAGLEGASSTEFDPNTAHPVISTMAEQVDVVAGDRDMGGTMRLGAYPAVLGEGTVVAGAYGAREVSERHRHRYEVNNAYRGRLSEAGLVFGGTSPDGTLVEFVELPADVHPFFVGTQAHPELKSRPTRPHPLFSAFADYCYALAAFESTA",
    "response#3": "LPDAYLSVTEALRAGGFAHRARVEIKWVASDECETPAGAAEALDGVDGVLIPGGFGIRGIEGKLGAIKHARTRGIPTLGLCLGLQAMVIEAARNMAGLEGASSTEFDPNTAHPVISTMAEQVDVVAGDRDMGGTMRLGAYPAVLGEGTVVAGAYGAREVSERHRHRYEVNNAYRGRLSEAGLVFGGTSPDGTLVEFVELPADVHPFFVGTQAHPELKSRPTRPHPLFSAFAVHIGLIGKYCSLHDAYLSVNEALKAGGYYFYKKVVVDYVDSTIITKDNVGEVLSKYDGIIVPGGFGDRGIEEMLLAIKYARENNVPLFGICLGMQLICIEYARNVIGYKDANSKEFDKNTTHPIIALLENQYSGIDMGGTLRLGLYDCEILSKDSKTFKAYQMDAIKERHRHRYEFNNKFLEVLHKDLVIAGKNPQADLVEIVELKGHPWFIGCQFHPEFLSRPQRPHPLFRDFLNWAAGR"
  },
  {
    "reference": "MTVYPITSKPTFPENAGTREYAASLDAADPLATFRDKFIVPSKANINHKKLAKPGLSSEPCIYFCGNSLGIQPKATARFLEAQLDTWSSIGVSGHFVDLEDSPLRQWQLLSEQAAGSMCKIVGAKPEEVAAMGTLTTNLHLLLASFYKPTATRHKILMDWKAFPSDHYAIESHIAWHDLDPNKSMVLIGPDEGEYEISTEKILSYIDQHADDAAVILLPGIQYYTGQLFDIPRITEYAQSRNIIVGWDLAHAFANVELKLHDWNVDFAAWCTYKYGNSGPGTMGGLFVHERHGQVDYSKGEDAPSFRHRLTGWYGGDRSVRFKMDNKFKPIPGAGGFQISNPSAIDLTALCAALSVFDETSMTELRKKSVMLTAYLEHLLLEGTSDETRPFSIITPSNHEARGAQLSLLLKPGLLQGVSKKLQEAAVVCDKREPGVVRVAPVPLYNTFSEVWTFVQQFKSALE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively.2. The protein should be able to bind pyridoxal 5'-phosphate ligand in a variety of conditions3. The designed protein should be involved in 'de novo' NAD biosynthetic process from tryptophan, tryptophan catabolic process by localizing to cytoplasm and exhibiting 3-hydroxykynureninase activity, kynureninase activity.4. A protein should be able to manipulate the activity of enzymes in the Cofactor biosynthesis, quinolinate from L-kynurenine: step 2/3 pathway, either by activating or inhibiting their function.5. The presence of Aminotransferase class V domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MSQPKMAKKYIRRRNNIEGLMANVVGAKAEEVALMNGLTVNLHILLLSFYKPTAKRHKILLEDKAFPSDHYAVESQIQLKGYDPQQSMLLMKPRPGEEVLRTEDILDTIEKEGDSIAVVLFSGVQYYTGQLFDMATITNAGQNKGCYVGLDCAHAVGNAELKLHDWGVDFACWCSYKYVNSGAGGLAGAFIHEKNARTVKPALTIKQPKMAKKYIRRRNNIEGLMANVVGAKAEEVALMNGLTVNLHILLLSFYKPTAKRHKILLEDKAFPSDHYAVESQIQLKGYDPQQSMLLMKPRPGEEVLRTEDILDTIEKEGDSIAVVLFSGVQYYTGQLFDMATITNAGQNKGCYVGLDCAHAVGNAELKLHDWGVDFACWCSYKYVNSGAGGLAGAFIHEKNARTVKPALTIK",
    "response#2": "QPKMAKKYIRRRNNIEGLMANVVGAKAEEVALMNGLTVNLHILLLSFYKPTAKRHKILLEDKAFPSDHYAVESQIQLKGYDPQQSMLLMKPRPGEEVLRTEDILDTIEKEGDSIAVVLFSGVQYYTGQLFDMATITNAGQNKGCYVGLDCAHAVGNAELKLHDWGVDFACWCSYKYVNSGAGGLAGAFIHEKNARTVKPALTLGNLQPKMAKKYIRRRNNIEGLMANVVGAKAEEVALMNGLTVNLHILLLSFYKPTAKRHKILLEDKAFPSDHYAVESQIQLKGYDPQQSMLLMKPRPGEEVLRTEDILDTIEKEGDSIAVVLFSGVQYYTGQLFDMATITNAGQNKGCYVGLDCAHAVGNAELKLHDWGVDFACWCSYKYVNSGAGGLAGAFIHEKNARTVKPALTLSSLAAGPVAASARIIGAKPNEVALMNGLTVNLHLLLVSFYRPSESRYKILMEAKAFPSDRYAIKSQVRHHGYDPKETVIQLAPRKGEHCLRTEDILKTIEAEGDSIAVVLFSGVQFYTGQKFEMQRITDAGHSKGCIVGFDLAHAVGNVKLNVHEWGVDFACWCSYKYMNSGAGGLAGAFIHEKHNA",
    "response#3": "QPKMAKKYIRRRNNIEGLMANVVGAKAEEVALMNGLTVNLHILLLSFYKPTAKRHKILLEDKAFPSDHYAVESQIQLKGYDPQQSMLLMKPRPGEEVLRTEDILDTIEKEGDSIAVVLFSGVQYYTGQLFDMATITNAGQNKGCYVGLDCAHAVGNAELKLHDWGVDFACWCSYKYVNSGAGGLAGAFIHEKNARTVKPALTG"
  },
  {
    "reference": "MSMLPSFGFTQEQVACVCEVLQQGGNLERLGRFLWSLPACDHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHPKLQQLWLKAHYVEAEKLRGRPLGAVGKYRVRRKFPLPRTIWDGEETSYCFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQSNMGHARSSNYSLPGLTASQPSHGLQAHQHQLQDSLLGPLTSSLVDLGS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein's functionality will benefit from Basic and acidic residues, Polar residues compositional bias in its structure.2. The designed protein should contain at least one Homeobox domain that can specifically recognize and bind to DNA.3. The designed protein should be involved in regulation of synaptic assembly at neuromuscular junction, mesenchymal cell proliferation involved in ureter development, branching involved in ureteric bud morphogenesis, ureter smooth muscle cell differentiation, myotome development by localizing to transcription regulator complex, nucleolus, nucleus, nucleoplasm, cytoplasm and exhibiting chromatin binding, sequence-specific DNA binding, transcription coactivator binding, transcription cis-regulatory region binding. The designed protein sequence is ",
    "response#1": "MIPVFCVVEQLDGSLEYDNREEHAEFVLVRKDVLFSQLVETALLALGYSHSSAAQAL",
    "response#2": "MIPVFCVVEQLDGSLEYDNREEHAEFVLVRKDVLFSQLVETALLALGYSHSSAAQAV",
    "response#3": "PDFSSLPSFLPQTPSSRPMTYSYSSNLPQVQPVREVTFREYAIEPATKWHPRGNLAHCYSAEELVHRDCLQAPSAAGVPGDVLAKSSANVYHHPTPAVSSNFYSTVGRNGVLPQAFDQFFETAYGTPT"
  },
  {
    "reference": "MKLAVYGKGGIGKSTTSCNISIALAKRGKKVLQIGCDPKSDSTFTLTGFLIPTIIDTLQAKDYHYEDVWPEDVIYQGYAGVDCVEAGGPPAGAGCGGYVVGETVKLLKEFNAFYEYDVILFDVLGDVVCGGFAAPLNYADYCIIVTDNGFDALFAANRITASVREKARTHPLRLAGLIGNRTKKRDLIEKYVETCPMPILEVLPLIEDIRVSRVKGKTLFEMTESEPTLQFVCDFYLNIADQLLTQPEGVIPRELGDRELFNLLSNFYLNSSNSTNTTLKNETNLFDLV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit oxidoreductase activity, acting on iron-sulfur proteins as donors, metal ion binding.2. For general function, the protein need meet that The L component serves as a unique electron donor to the NB-component of the complex, and binds Mg-ATP.; Component of the dark-operative protochlorophyllide reductase (DPOR) that uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide)3. The protein should have a high affinity for [4Fe-4S] cluster, ATP. The designed protein sequence is ",
    "response#1": "PHVGAMRVATGMTGLHYVLHAPQGDTYADLLFTMIERRDHRPPVSYTTFQARDLGSDTAHLFKDSCRDAYERFKPEAIIVGASCTAELIQDDPGGLAETMGLPIPVIALELPSYQRKENFGCDETFFQIVRALAKPVEKTARVSCNILGPTGLGFRHRDDVEELTGLLSEMGVDVNVVAPMRSSPSDIARLGAAHFNVMLYPETCEAACRHLERAFQQPYTKTVPIGVGATRDFIAEVQGLTGVTGAPDENRLRLPWWSASVDSTYLTGKRVFLFGDATHVKASARIARDEMGFEVVGLGCYNREFARDIRKLAKEFGLEALITDDYLEVEKAIEEAAPEMILGTQMERHIGKRLGIPCAVISAPVHVQDFPARYSPQVGFEGANVIFDTWIHPG",
    "response#2": "PHVGAMRVATGMEGLHYVLHAPQGDTYADLLFTMIERRNKRPPVTYTTFAARDLGKDTAELFMKAARDAYERFKPQAMIVGASCTGSLIQDDPGGLAKSLGFPIPVVAIDLPAYQRKENWGAAETLYQLVRALAGPKAPAPGAKRPERAPGVRPTCNLLGPTALGFRHRDDITEITGLLGKLGIDVNVVAPMGATPADIARLGDADFNVVMYPEIAGQAASWLHRIFHQPFTKTVPIGVSATRDFIKEVAQLAGVDATAVLEAASTRLPWYSHSVDSTYLTNKRVFIFGDATHAIAAARIASEELGFKVVGLGSYSREFGRELREAAKHYDVEPLITDDYLEVEAKVAELHPELVLGTQMERHIAKRLGVPCAVISAPVHVQDFPARYAPQMGFEGANVIFDTWVHPD",
    "response#3": "PHVGAMRVATGMTGLHYVLHAPQGDTYADLLFTMIERRDHRPPVSYTTFQARDLGSDTAHLFKDSCRDAYERFKPEAIIVGASCTAELIQDDPGGLAETMGLPIPVIALELPSYQRKENFGCDETFFQIVRALAKPVEKTARVSCNILGPTGLGFRHRDDVEELTGLLSEMGVDVNVVAPMRSSPSDIARLGAAHFNVMLYPETCEAACRHLERAFQQPYTKTVPIGVGATRDFIAEVQGLTGVTGAPDENRLRLPWWSASVDSTYLTGKRVFLFGDATHVKASARIARDEMGFEVVGLGCYNREFARDIRKLAKEFGLEALITDDYLEVEKAIEEAAPEMILGTQMERHIGKRLGIPCAVISAPVHVQDFPARYSPQVGFEGANVIFDTWIHPG"
  },
  {
    "reference": "MAHRNITGTFRLLLPLLALLFTAVGASCSNNTKSATQESGDSIINKSNMTTRTFTRPDDATLRRQLTAEQYAVTQQAATERPFTNQYDNEFRPGIYVDITTGEPLFSSTDKYDSGCGWPAFSKPIDKRLVVEKADNSHGMQRTEVKSRTGNAHLGHVFNDGPSDKGGLRYCINSASLRFVPLAEMEKEGYGEYTKLVRREKEIYVAGGCFWGTEHYIKQVKGVLSTEVGYANGNTKNPTYKEVCTDATGFAEAVHIKYDPDVVSLDFLVKLYFVSIDPTSHNQQGNDRGSQYRTGVYYTDPADRAVIKKVFDAEERKHSVPLEVELLPLKNFYPAEEYHQDYLEKNPTGYCHLPSSLFEYARKAKQR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.2. For general function, the protein need meet that Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.3. The Nucleophile active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.4. The protein contains novel MsrB domains that confer a unique function or activity.5. The protein design must be able to perform peptide-methionine (R)-S-oxide reductase activity in order to contribute to protein repair.6. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "LSGMQYRVTQEDGTEPPFVNAYDDNKEAGIYVDVVSGEPLFSSLDKYDSGTGWPSFTRPLEPSLIVTRPDPGLLGDRTEVRSRVADSHLGHVFPDGPAPTGLRYCMNSAAMRFIPDDATLRRILTPEQYHVTQEAGTEYAFSHEYDHLFDPGIYVDIVSGQPLFSSRDKYDSQCGWPSFTRPIRPDAVTRRDDFSYHMHRIEVRSSAADSHLGHVFDDGPKDRGGLRYCINGASLRFIPEAHMRQQGW",
    "response#2": "MPEIYLAGGCFWGTEKYLSSIRGVLSTQVGYANGKTENPTYEEVCSQNTGHAEAVRVLYDARVIPLAFLLELYYESLDPVSVNRQGGDHGTQYRTGIYYVDDKDLHVIQRSIAQLQERYGKPIAIEVEPLCNFSPAEEYHQKYLDKHPDGYCHISTAP",
    "response#3": "LSGMQYRVTQEDGTEPPFVNAYDDNKEAGIYVDVVSGEPLFSSLDKYDSGTGWPSFTRPLEPSLIVTRPDPGLLGDRTEVRSRVADSHLGHVFPDGPAPTGLRYCMNSAAMRFIPDADALRTTLTAAQYEVTQKSATEPPFQNEFWNTFQPGIYVDITTGEPLFTSSDKFESGCGWPSFSKPIDPDVIHEKKDTSHGMQRTEVRSRAGNAHLGHVFNDGPKKTGGLRYCINSASLRFIPRE"
  },
  {
    "reference": "MGGKWSKHSGWAVVREKMKRATPADEPAADGVGAVSRDLEKHGAITSSNTAANNAACAWLEAQEDEEVGFPVRPQVPLRPMTSRAARDLSHFLRKEGGLDGLIHSQKRQEILDLWVYHTQGYFPDWQNYTPGPGIRYPLTLGWCYKLVPVDTERVEAANEGENNSLLHPMSQHGMDDPEREVLQWRFDSTLAFHHVARELHPEYFKNC",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.2. The protein's functional design necessitates the inclusion of a Dileucine internalization motif; necessary for CD4 internalization motif.3. The protein should be able to modulate suppression by virus of host autophagy in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "VPAAAGAA",
    "response#2": "MSWFQSMF",
    "response#3": "SWSS"
  },
  {
    "reference": "MTDHSNTRVVVGMSGGVDSSVVALLLKQQGYDVVGVFMKNWDDTDENGVCTATEDYKDVAKVADKIGIPYYSINFEKEYWDRVFTYFLDEYKKGRTPNPDVICNKEIKFKAFLDYAMDLGADYIATGHYARLQHDEDGTMHLLRGVDSNKDQTYFLSQLDSKTLQKVMFPLGEMVKPDVRKLALNAGLATAKKKDSVGICFIGEKNFKEFLGHYLPATPGKMMTFDGQVKGEHAGLMYYTIGQRRGLGIGGDGEDNEPWFVVGKDLKKNILYVGKGYHNPHLYATHLKASDLHFVTDEDLGNDFHVTAKFRYRQKDSGVTVHFNDDHTEVTVTFDDPVRAITPGQAVVFYNGEECLGSGMIDAAYNDERVLQYV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be optimized for sulfurtransferase activity activity in order to improve tRNA modification.2. The ATP binding site should be stable and able to withstand changes in environmental conditions.3. We need a protein that can perform AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in   tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-   cysteinyl-[protein] with high efficiency. The designed protein sequence is ",
    "response#1": "MSWLKDWNIVDESRLLGCDDVIVKIRYRKQENHCSVTITPEGLLHIRLHEPLSAIAEGQAAAFYKDGLLLGGGIA",
    "response#2": "WLKDWNIVDESRLLGCDDVIVKIRYRKQENHCSVTITPEGLLHIRLHEPLSAIAEGQAAAFYKDGLLLGGGIF",
    "response#3": "HVSSEDFNFLVPVEKWKKEGLSVQGRYRQKAVKIKDVSIEGNRLTAYFEKPEERFAKGQVLAVYDGDILLGGGIL"
  },
  {
    "reference": "MDATRKPLRIPPAMALYAEEQGVFDIIQKMVKKVLVDRPKDPIQYMIDHLSNDNDDVPRVFILGPPASGKHTMAKLLCKRLNATHLTPESVLSSDVSLLAKEAQSYRDKGQEVPDELWAKLMQQRLSKVDCIKRGWILEGFPKTREQALKLQMAGICPDHLVVLDAPDIVLIERNMGKRIDTANGEVYHTTFDWPSDPTVQRNLVEPEGISEEETGLRLIEYHRNIPGILRTYPKTSKKINADQPYMDVFSQVLTFVLSKPRSLAPHTPRILLYGPPGSGRSLQASLLAQKYGIVNICCGQVLKEAVADQTKLGEVIQPYIENDQQVPDNLVLKILTEHLSSLESATHGWVLHGFPRDTDQAALLKDAGFVPNRVFSLDLSDDVVIERLSLCMTDPVSGERYHDIYKPAPSSEVHERLQQNPRHSEQRVQARLDMYHANAEELDEFYPDVIHINADQDPYTVFEFIESYTVSPLPRPLPEEPTSP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein have enhanced a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-   triphosphate + ADP.2. The ATP, AMP binding site should be stable and able to withstand changes in environmental conditions.3. A protein that contributes to nucleoside triphosphate biosynthetic process, nucleoside diphosphate phosphorylation by functioning in cytosol with nucleoside diphosphate kinase activity, cytidylate kinase activity, ATP binding.4. For general function, the protein need meet that Also displays broad nucleoside diphosphate kinase activity.; Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates; Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP The designed protein sequence is ",
    "response#1": "KPVCFVVFGKPGVGKTTLARYITQAWKCIRVEALPILEEQIAAETESGVMLQSMLISGQSIPDELVIKLMLEKLNSPEVCHFGYIITEIPSLSQDAMTTLQQIELIKNLNLKPDVIINIKCPDYDLCQRISGQRQHNNTGYIYSRDQWDPEVIENHRKKKKEAQKDGKGEEEEEEEEQEEEEAFIAEMQMVAEILHHLVQRPEDYLENVENIAI",
    "response#2": "KPVCFVVFGKPGVGKTTLARYITQAWKCIRVEALPILEEQIAAETESGVMLQSMLISGQSIPDELVIKLMLEKLNSPEVCHFGYIITEIPSLSQDAMTTLQQIELIKNLNLKPDVIINIKCPDYDLCQRISGQRQHNNTGYIYSRDQWDPEVIENHRKKKKEAQKDGKGEEEEEEEEQEEEEAFIAEMQMVAEILHHLVQRPEDYLENVENIVS",
    "response#3": "KPVCFVVFGKPGVGKTTLARYITQAWKCIRVEALPILEEQIAAETESGVMLQSMLISGQSIPDELVIKLMLEKLNSPEVCHFGYIITEIPSLSQDAMTTLQQIELIKNLNLKPDVIINIKCPDYDLCQRISGQRQHNNTGYIYSRDQWDPEVIENHRKKKKEAQKDGKGEEEEEEEEQEEEEAFIAEMQMVAEILHHLVQRPEDYLENVENIVSTC"
  },
  {
    "reference": "MTIGLSVTNDVFARDILTVAQLNQAVGQLLERSIPSLWVRGEISNFTQAASGHWYFTLKDSRAAVRTVMFRSRAAQVGFVPRPGDQVEVRARVSLYEPRGDYQLQADGMRRAGVGNLYEAFLRLKAQLQDEGLFDPQRKRQPARLPRAIGVVTSLHAAALRDVLSALARRAPQVPVIIYPAPVQGADAAARLAARVAQANQRAEVDTLLLVRGGGSIEDLWSFNDEALAREVAASDIPVISGVGHETDFTIVDFVADLRAPTPTAAAELACVPRGDLLAALRHTAERLARAQQRRLDQAAQRLDRAAAMLTSPAQRLAHQQERLNTLRHRLASAWRGPQGRRVARLDMLAQRLAHRRPDTGRAAERSAALLAQLGRAQARLAAARQARLDTLAAQLRALDPQHTLARGYAIVRDAAGAIVTDATRLAARDRIEIAVARGRIGADVTDIGTPDGTDGNPALRRG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be designed to catalyze the following reaction Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-   direction to yield nucleoside 5'-phosphates..2. For general function, the protein need meet that Bidirectionally degrades single-stranded DNA into large acid- insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.3. The ideal protein would be one that plays a critical role in DNA catabolic process through exodeoxyribonuclease VII activity, nucleic acid binding within cytoplasm, exodeoxyribonuclease VII complex. The designed protein sequence is ",
    "response#1": "YFASELKKPLPDFIQTIALVTSQTGAALQDMLRIIQNRWPLVKVYVVDTLVQGSDAAPMIARSIAYADGLGVDVIVVGRGGGSLEDLWPFNEEIVADAIFEAKTPIVSAVGHEIDFLISDFVADLRAPTPSAAMEMILPDRQEMLMHLDLLMQRLTKRMQTILQLKTQELGHLQNSLFQLSPQKRLEFYEKEITIMNERMNETITAILKNSSHEIPHLKALFDQKIEWIWKQKKQDLTSLQQKLTMTMEAL",
    "response#2": "LFSQVYKKTIPPYAKTIGVITSPTGAAIRDIITTIKRRYPIGNVIVFPVLVQGESAAPSIVQAIRTANEMEEIDVLIVGRGGGSIEELWAFNEEMVARAIFKSEIPIISAVGHETDFTIADFVADLRAPTPTAAAELAAPNIIELQEKVLQRTLRLQRAMRELVHKKEEKLQVLQKSYAFRYPRQVYEQKEEQLDRALEQLVLAKERYIDKKVNQLKQLSFYLEKHHPSQKIMQTKVAVETLQKQLQREMQTLLQAKEFAFVRAAQKLEALSPLKVMMRGYGLVYDEEKQVLKSVKDVSLGDAVSVQLQDGILDARVE",
    "response#3": "YFASELKKPLPDFIQTIALVTSQTGAALQDMLRIIQNRWPLVKVYVVDTLVQGSDAAPMIARSIAYADGLGVDVIVVGRGGGSLEDLWPFNEEIVADAIFEAKTPIVSAVGHEIDFLISDFVADLRAPTPSAAMEMILPDRQEMLMHLDLLMQRLTKRMQTILQLKTQELGHLQNSLFQLSPQKRLEFYEKEITIMNERMNETITAILKNSSHEIPHLKALFDQKIEWIWKQKKQDLTSLQQKLTMTMEAV"
  },
  {
    "reference": "MTSGKNGITYAEAGVDIDAGNALVDRIKPAAKRTNRPGVASGLGGFGALFDLKAAGYQDPVLVAATDGVGTKLRIAIDTGLVDGVGIDLVAMCVNDLVCQGAEPLFFLDYFATGKLETDTAARIIEGIAEGCVQSGCALIGGETAEMPGMYPAGDFDLAGFAVGAMERGTALPEGVVEGDVLLGLASNGVHSNGYSLVRKLVEISGNTWESDCPFGDGKLGQALLTPTRLYVRQVLAAIRAGGVHALAHITGGGLTENLPRVLPEGMGATIDLDTWDLPPVFGWMAETGGIAEAEMLKTFNCGIGMIVVCAADRAEALAELLSAEGETVARIGTVTTTPGIAYTGKLL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be optimized for activity in Purine metabolism, IMP biosynthesis via de novo pathway pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The protein design should aim to mimic or enhance the function of natural proteins involved in 'de novo' IMP biosynthetic process, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "QPGDLLVGLPSSGLHCAGFNEILTQLVALKVNLKELSPVEGGEDGLSLAQVLGTPTRLYVKELLPHLQKGEEIKSLVQVTHGLIKDVQRLLPEGFETTLDFGAVPVPKIFGWLAGKLKLSAQTLLEKHNCGIGMVMILPQTC",
    "response#2": "MASVKDRRVESPATDTDLGRGVFEFSDRYSVFDWGEMPDHVPGKGASLCTMGAYTFEQLAAAGVPTHYQGVRTPDGETVRLADAPEAPTQMVIDLTQVPTLPFEDGSYDYDRYHDAGGSNYLVPLEVVFRNAVPVGSSLRTRCAPADVGIDADEWPQGPVELPETIVEFSTKYEEQDRYLSRSMADEIAGDADIAELDALARRVNETITDCAADAGFVHDDGKLECVYVDGEVRVADVAGTFDENRFRFDGREVSKEAVRQFYKRSDPEWVGAVKDAKRAADERGVADWKSLCEPSPDPLPPEILQAAADLYAAGANRYTN",
    "response#3": "QPGDLLVGLPSSGLHCAGFNEILTQLVALKVNLKELSPVEGGEDGLSLAQVLGTPTRLYVKELLPHLQKGEEIKSLVQVTHGLIKDVQRLLPEGFETTLDFGAVPVPKIFGWLAGKLKLSAQTLLEKHNCGIGMVMILPQTC"
  },
  {
    "reference": "MNKGIIGKKLGMTQIFLEDGTRVPVTVVQAGPCVVLQKKTAEVDGYSAVQVGFETVNAAKANSADRGHCVKAGKGVFRHLRELKLEQEAELNIGDELTVQQFEPGDLIDVTGTSIGKGFQGVIKRHNFKGGRASHGSRFHRAPGSIGCSATPSRVFKNKKMPGQMGNERVTVQRLQVVRVDADQNLILIKGAIPDSKNNVVVIKDSVKATK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein can be expressed in ribosome and perform structural constituent of ribosome without triggering undesirable cellular responses.2. The protein must exhibit the following characteristics: One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.. The designed protein sequence is ",
    "response#1": "MLPVE",
    "response#2": "MES",
    "response#3": "MF"
  },
  {
    "reference": "MPVKLFLASSNPGKLKEYRVLAEADAPASASIELAPVPEFASLTEFEENAPTFAENAAGKALHYSRLLEGLVFADDSGLVVPSLGGAPGVHSARYAGVDAPNSKRIEKLLNEMRDKTGEQRAAHFVCAIALARQGRALAVVTGRVDGEILGSTRGAGGFGYDPVFYFPPLKKTFAELSAEEKNGLSHRGKAFRKLLVALSSML",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.2. The designed protein must be optimized for dITP diphosphatase activity, metal ion binding. The designed protein sequence is ",
    "response#1": "MPN",
    "response#2": "MKLAFVTDSYLPR",
    "response#3": "MLRFLH"
  },
  {
    "reference": "MALPFVLLMALVVLNCKSICSLGCDLPQTHSLSNRRTLMIMAQMGRISPFSCLKDRHDFGFPQEEFDGNQFQKAQAISVLHEMIQQTFNLFSTKDSSATWDETLLDKFYTELYQQLNDLEACMMQEVGVEDTPLMNVDSILTVRKYFQRITLYLTEKKYSPCAWEVVRAEIMRSFSLSANLQERLRRKE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Produced by macrophages, IFN-alpha have antiviral activities.2. The designed protein should be involved in T cell activation involved in immune response, humoral immune response by localizing to extracellular space, extracellular region and exhibiting cytokine activity, cytokine receptor binding. The designed protein sequence is ",
    "response#1": "PDPPKNLQLKPLESPRHVEVSWEYPDSWSTPHSYFSLTFAVQVQGKNKREKKDKVFTDKTSATVICQKHANVRVQAQDRYYSSSWSEWASC",
    "response#2": "KTYLKCEAKNYSGTFTCSWLTESSPDLKFSIRSSKGSSDPRGVTCGPAVLSEEKVKIDNKEYQKYTADCQDVSACPSAEESQPIEVVLDAIL",
    "response#3": "PDPPKNLQLKPLESPRHVEVSWEYPDSWSTPHSYFSLTFAVQVQGKNKREKKDKVFTDKTSATVICQKHANVRVQAQDRYYSSSWSEWASC"
  },
  {
    "reference": "MSAGGEHLKDEGTRRQVVLAGGIAGLISRFCIAPLDVVKIRLQLQIHSLSDPTSHAHITGPVYKGTLSTIKTILREEGLTGLWKGNIPAELLYVCYGGIQFTTYRTTTQLLAQLDPHRLPQPIESFISGALGGGIATAATYPLDLLRTRFAAQGSGDNRVYESLFASLRDIAKTEGTVGFFRGCSAAVGQIVPYMGLFFATYEALRPVMATAPELSPIPLPPGSGDAAAGIVASVLAKTGVFPLDLVRKRLQVQGPTRALYVHRNIPEYRGVFNTMGLIFRTQGLRGLYRGLTVSLVKAAPASAVTMWTYERALKLLREHEIAAGRDE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have a Helical; Name=6, Helical; Name=4, Helical; Name=3, Helical; Name=2 domain that is long enough to span the entire membrane.2. The protein should be designed to localize specifically to mitochondrial inner membrane in order to perform its role in thiamine pyrophosphate transmembrane transport more effectively. The designed protein sequence is ",
    "response#1": "LSGCASGLVRVFLTSPTEVAKVRLQTQAQSQTQQRRPSASWTSVAPALCPAPTACLEPRPKYSGPLHCLVTVAREEGLRGLYKGSSALLLREGHSFATYFLSYAVLSEWLG",
    "response#2": "PTKADITLSGCASGLVRVFLTSPTEVAKVRLQTQAQSQTQQRRPSASWTSVAPALCPAPTACLEPRPKYSGPLHCLVTVAREEGLRGLYKGSSALLLREGHSFATYFLSYAVLSEQ",
    "response#3": "PTKADITLSGCASGLVRVFLTSPTEVAKVRLQTQAQSQTQQRRPSASWTSVAPALCPAPTACLEPRPKYSGPLHCLVTVAREEGLRGLYKGSSALLLREGHSFATYFLSYAVLSEL"
  },
  {
    "reference": "MKTLLVLNSSGRVTRSLTRRLTSRFAEAWSAVHHDAVVVQRDLTLNPPPTINEPWIVAAFAAPDTPATVREAVLRASDELLDELTAADAVVIGAPVYNFGLPAQLKAYVDQIVRVGRSFALTGDAAVPYRALLAPKPVVVMTAASDGVMLPGGALAHLNLVEPHLTAALGFIGLTDVRFVRVADSVADQAAHPHSLAAAERAIEMILPRLAAA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Also exhibits azoreductase activity.2. The protein must be able to electron transfer activity, FMN binding.3. The protein should have a low Km value for anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD(+) =   2-(4-dimethylaminophenyl)diazenylbenzoate + 2 H(+) + 2 NADH.4. The protein must bind to FMN in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MKLLHLDSSPRGERSISRSLTQQFVSLWKQMHLDVPVIYRDLGRYPVPAIDEAWIAAAFCPPAQLTPELQSALMISDELIAELLAANLYIFGIPMYNYSVPASFKAYIDQIVRVRRTFVVSADGYEGLLKDKKVLVITTRGGSYAGEPLDFQEPYLRAVFGFIGITDVTFIHAENLAIGSEERQLAIATAHEAIQR",
    "response#2": "MKLLHLDSSPRGERSISRSLTQQFVSLWKQMHLDVPVIYRDLGRYPVPAIDEAWIAAAFCPPAQLTPELQSALMISDELIAELLAANLYIFGIPMYNYSVPASFKAYIDQIVRVRRTFVVSADGYEGLLKDKKVLVITTRGGSYAGEPLDFQEPYLRAVFGFIGITDVTFIHAENLAIGSEERQLAIATAHEAIQR",
    "response#3": "MKLLHIDSSIAGVGSVTRELSAEIVALLKGADKSWNVIYRDLVAEPLSHLTGGYLAALQGAPADQAVQADVGAGLAVMEDFLAADVIVIGAPMYNFGVPSQLKAWIDRLAVPGKTFSYGEHGPVGLCKGKKVIVASARGGIFSAGSPLAALDHQENYLLSFFGFLGIAEISFVRAEGVALGPVVRAKAIAAAKEHAAELLA"
  },
  {
    "reference": "GFNPCGETCWNKPTCHAPGCTCSIANICVRN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design must be focused on enhancing the efficiency of defense response.2. For general function, the protein need meet that Probably participates in a plant defense mechanism. The designed protein sequence is ",
    "response#1": "TTMMEQVLEFLSEEEERGIIGVYGPGGVGKTTLMQSINNELITKGHQYDVLIWVQMSREFGECTIQQAVGARLGLSWDEKETGENRALKIYRALRQKRFLLLLDDVWEEIDLEKTGVPRPDRENKCKVMFTTRSIALCNNMGAEYKLRVEFLEKKHAWELFCSKVWRKDLLESSSIRRLAEIIVSKCGGLPLALITLGGAMAHRETEEEWIHASEVLTRFPAEMKGMNYVFALLKFSYDE",
    "response#2": "LGELGNLNLYGSIKISHLERVKNDKDAKEANLSAKGNLHSLSMSWNNFGPHIYESEEVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILISNFRNCSCLPPFGDLPCLESLELF",
    "response#3": "AEAFIQVLLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAATYEVDDILDEYKTKATRFSQSEYGRYHPKVIPFRHKVGKRMDQVMKKLKAIAG"
  },
  {
    "reference": "MLKVNNVFGSNPNRMTKLEDEHYFIDDIVSIKNRQKSKMYVREGKRIGHGSFGTVTQSILSSNSIEWLGPYAIKRVVKSPKVQSLELEILQNIRHPNLVTLEFFFESHCTTKDGGHLYQKNFVMEYIPQTLSSEIHEYFDNGSKMPTKHIKLYTFQILRALLTLHSMSICHGDLKPSNILIIPSSGIAKVCDFGSAQRLDDNTELKTYFCSRFYRAPELLLNSKDYTTQIDIWSLGCIIGEMIKGQPLFKGDSANSQLEEIAKLLGRFPKSSIKNSQELQDSLNDQKFKKFMHWFPSIEFFDVEFLLKVLTYDATERCDARQLMAHEFFDALRNETYFLPRGSSMPVHLPDLFNFSASEKRALGEYYNLIVPSLD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The ideal protein would be one that plays a critical role in protein phosphorylation, proteolysis through protein serine/threonine kinase activity, protein serine kinase activity, cyclin-dependent protein serine/threonine kinase activity within mediator complex, nucleus.2. The protein must exhibit the following characteristics: Regulates activity of MSN2, a transcription factor that binds to the stress-response element (STRE); Probably promotes formation of a complex between MSN2 and DNA; Required for heat stress-instigated phosphorylation of BCY1 which is involved in cell wall integrity signaling.3. The designed protein should have a binding site that is specific to the target ATP ligand. The designed protein sequence is ",
    "response#1": "IETQEERQGKMLLHTEYSLLSLLHNQEGVVHHHGLFQDRACEIIEDLEANRMVRKMKKRICLVLDCLCAHDFSDKTADLINLQHYVIKEKRLSERETVVIFYDVVRVVEALHKKNIVHRDLKLGNMVLNKRTHRITITNFCLGKHLVSEDDLLKDQRGSPAYISPDVLSGRPYRGKPSDMWALGVVLFTMLYGQFPFYDSIPQELFRKIKAAEYTIPEDGRVSENTVCLIRKLLVLDPQQRLTASEEIETQEERQGKMLLHTEYSLLSLLHNQEGVVHHHGLFQDRACEIIEDLEANRMVRKMKKRICLVLDCLCAHDFSDKTADLINLQHYVIKEKRLSERETVVIFYDVVRVVEALHKKNIVHRDLKLGNMVLNKRTHRITITNFCLGKHLVSEDDLLKDQRGSPAYISPDVLSGRPYRGKPSDMWALGVVLFTMLYGQFPFYDSIPQELFRKIKAAEYTIPEDGRVSENTVCLIRKLLVLDPQQRLTASEEIETQEERQGKMLLHTEYSLLSLLHNQEGVVHHHGLFQDRACEIIEDLEANRMVRKMKKRICLVLDCLCAHDFSDKTADLINLQHYVIKEKRLSERETVVIFYDVVRVVEALHKKNIVHRDLKLGNMVLNKRTHRITITNFCLGKHLVSEDDLLKDQRGSPAYISPDVLSGRPYRGKPSDMWALGVVLFTMLYGQFPFYDSIPQELFRKIKAAEYTIPEDGRVSENTVCLIRKLLVLDPQQRLTASEEIETQEERQGKMLLHTEYSLLSLLHNQEGVVHHHGLFQDRACEIIEDLEANRMVRKMKKRICLVLDCLCAHDFSDKTADLINLQHYVIKEKRLSERETVVIFYDVVRVVEALHKKNIVHRDLKLGNMVLNKRTHRITITNFCLGKHLVSEDDLLKDQRGSPAYISPDVLSGRPYRGKPSDMWALGVVLFTMLYGQFPFYDSIPQELFRKIKAAEYTIPEDGRVSENTVCLIRKLLVLDPQQRLTASEE",
    "response#2": "IETQEERQGKMLLHTEYSLLSLLHNQEGVVHHHGLFQDRACEIIEDLEANRMVRKMKKRICLVLDCLCAHDFSDKTADLINLQHYVIKEKRLSERETVVIFYDVVRVVEALHKKNIVHRDLKLGNMVLNKRTHRITITNFCLGKHLVSEDDLLKDQRGSPAYISPDVLSGRPYRGKPSDMWALGVVLFTMLYGQFPFYDSIPQELFRKIKAAEYTIPEDGRVSENTVCLIRKLLVLDPQQRLTASEEYDVERTLGKGNFAVVKLARHRVTKTQVAIKIIDKTRLDSSNLEKIYREVQLMKLLNHPNIIKLYQVMETKDMLYIVTEFAKNGEMFDYLTSNGHLSENEARKKFWQILSAVEYCHNHHIVHRDLKTENLLLDGNMDIKLADFGFGNFYKPGEPLSTWCGSPPYAAPEVFEGKEYEGPQLDIWSLGVVLYVLVCGSLPFDGPNLPTLRQRVLEGRFRIPFFMSQDCETLIRRMLVVDPAKRITIAQIRQHRWRWHFGIRSRSYPLDVMGEIYRALKNLGAEWAKPTEEELWTIRVRWKYDTSAQFECGSAPNLMKMQIQLFQLEPNNYLVSFKFSGWESAHGNAGTDSPQSHRQQDLDEVGSFSAYPFLHLATRLIMEAQ",
    "response#3": "NHNSLGDGNFSVVKECMNIHTKDLYAMKLIKKQTVKNKIQLIQREFDLLRSISEKIRDMEKKNEHSLDIFEGHHHILQLFDYFETADNIVLITQLCQKGDLYEKIVENQCLDLETQVTSYCACLVSVLEFLHSQGIVHRDLKAENVLFRLRVNENEKNLQGEHHGDFKYDLLAHDLVLADFGLAAEYNTSKVNSLKEFVGTISYIAPEIVKCKGVGEMTPDQVGKLDKYGCPVDIWALGVLTYFMAFGYTPFDCTTDDETLECISKCDYYVDEQMMHDPKYEQFWNFVQCCFTIDPAVRRSAKNLKQHPFF"
  },
  {
    "reference": "MDFLHLGSFVFSSRLILGTGKFSNADLMIEAIKASGAQLVTVALRRFNREQIADDLFGPLSALQGITLMPNTSGASTAREAIHAAHIARELSGSPFIKVEIHPNPQHLMPDALETWEASRILAKEGFLVMPYIPADPVLAKRLEEVGCASVMPLGSAIGSGQGLANAGMIELIIRESGIPVIVDAGLRAPSEAAAAMEMGCGAVLVNSAVAVAGNPPEMANAFAEAVRAGRRAFKAELMPKSSFALSTSPLTTFLGKKS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a high affinity for 1-deoxy-D-xylulose 5-phosphate.2. The designed protein must have a Schiff-base intermediate with DXP that is structurally stable and resistant to denaturation.3. The protein must exhibit the following characteristics: In vitro, sulfur can be provided by H(2)S.; Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS.4. The protein should be optimized for stability and expression in cytoplasm where it will participate in thiamine diphosphate biosynthetic process. The designed protein sequence is ",
    "response#1": "IGAHAFTSRLLVGTGKYPDFPTMQRALAASGAEVVTVAVRRLDLSKKGEESLLAWIPKGMKLLPNTAACFTAEEAIRTARLGRELEMGDLVKLEVIGDRRTLFPDVEGLIQAAKVLVKEGFTVLPYTNDDPVTAKKLEDAGCAAVMPLGAPIGSGLGLRNPYNLRIIMETVQVPVLVDAGVGTASDAALAMELGAVAVLMNTAIAEAKDPVLMAEAMRAGVEGGRKAFLAGRIPMKLHAAASSN",
    "response#2": "IGAHAFTSRLLVGTGKYPDFPTMQRALAASGAEVVTVAVRRLDLSKKGEESLLAWIPKGMKLLPNTAACFTAEEAIRTARLGRELEMGDLVKLEVIGDRRTLFPDVEGLIQAAKVLVKEGFTVLPYTNDDPVTAKKLEDAGCAAVMPLGAPIGSGLGLRNPYNLRIIMETVQVPVLVDAGVGTASDAALAMELGAVAVLMNTAIAEAKDPVLMAEAMRAGVEGGRKAFLAGRIPMKLHAAASSR",
    "response#3": "IGAHAFTSRLLVGTGKYPDFPTMQRALAASGAEVVTVAVRRLDLSKKGEESLLAWIPKGMKLLPNTAACFTAEEAIRTARLGRELEMGDLVKLEVIGDRRTLFPDVEGLIQAAKVLVKEGFTVLPYTNDDPVTAKKLEDAGCAAVMPLGAPIGSGLGLRNPYNLRIIMETVQVPVLVDAGVGTASDAALAMELGAVAVLMNTAIAEAKDPVLMAEAMRAGVEGGRKAFLAGRIPMKLHAAASSA"
  },
  {
    "reference": "MGPKLLESRLCLLLLLGLVLMLASCLGQTPSQKFAIQHINNNTNLQCNVEMMRINRARRTCKGLNTFLHTSFANAVGVCGNPSGLCSDKRSQNCHNSSSRVHITVCNITSRATNYTQCRYQSRRSLEYYTVACDPRTPQDSPMYPVVPVHLDGTF",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Possesses a wide variety of biological activities . The designed protein sequence is ",
    "response#1": "PISPVQGFFLDLGLAGQIGLINYIFEVEGSDLKHGLAHVTNLLESKNPVFRTLIEKTKESGFVQILVAKTRSSWFYPSDLRLYLEKTMTQKFELGKPAVQYALVMEDAAHEGRNFFVISMHHTHCDAFSRFLIGKEISQILESPSYYARSENIERPWFGNYVKHVQQKATDDKASLFWDAYMCGANLANIYPLHRATLNGEFDGAIIEKIQAPVATRIADGSPRNSTQVILAAWAIALANLSGLRDITFGLCRHGRSSSSFRDVRRLMGPLVNVLPFRVSLICNEEPAPALLQRIQNEITSTNKHEHGC",
    "response#2": "PISPVQGFFLDLGLAGQIGLINYIFEVEGSDLKHGLAHVTNLLESKNPVFRTLIEKTKESGFVQILVAKTRSSWFYPSDLRLYLEKTMTQKFELGKPAVQYALVMEDAAHEGRNFFVISMHHTHCDAFSRFLIGKEISQILESPSYYARSENIERPWFGNYVKHVQQKATDDKASLFWDAYMCGANLANIYPLHRATLNGEFDGAIIEKIQAPVATRIADGSPRNSTQVILAAWAIALANLSGLRDITFGLCRHGRSSSSFRDVRRLMGPLVNVLPFRVSLICNEEPAPALLQRIQNEITSTNKHEHGF",
    "response#3": "PISPVQGFFLDLGLAGQIGLINYIFEVEGSDLKHGLAHVTNLLESKNPVFRTLIEKTKESGFVQILVAKTRSSWFYPSDLRLYLEKTMTQKFELGKPAVQYALVMEDAAHEGRNFFVISMHHTHCDAFSRFLIGKEISQILESPSYYARSENIERPWFGNYVKHVQQKATDDKASLFWDAYMCGANLANIYPLHRATLNGEFDGAIIEKIQAPVATRIADGSPRNSTQVILAAWAIALANLSGLRDITFGLCRHGRSSSSFRDVRRLMGPLVNVLPFRVSLICNEEPAPALLQRIQNEITSTNKHEHGL"
  },
  {
    "reference": "MKTAYDVILAPVLSEKAYAGFAEGKYTFWVHPKATKTEIKNAVETAFKVKVVKVNTLHVRGKKKRLGRYLGKRPDRKKAIVQVAPGQKIEALEGLI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must be able to rRNA binding. The designed protein sequence is ",
    "response#1": "LITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSC",
    "response#2": "LITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSC",
    "response#3": "PKILITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSLQKGTFS"
  },
  {
    "reference": "MSDHRILPKVHHLPQALYRAEQVRELDRLAIEEYGIPGETLMERAGKAAWELLEQRWPAASRLVVLVGTGNNGGDGFVVARLAHQAGRKVTVLQLGDRDRLSGDARTNALRYAALSGDWRAFEGILPENADLLVDGIFGTGLEREVADTWAEAIRAVNGHRTPVLALDLPSGLHADTGAVLGTAVRAEATISFIGLKQGMFTGEGPACCGEIFFDSLEVPARLYGHLVISARRIDWARQRDLLQPRVATAHKGHFGHVLVIGGDRGMGGAARLAAEAALRTGAGLVSLATRPEHVVPVLATRPEIMAHGIEDPLQLDPLLRHSTHIAIGPGLGQSDWGRALFEMAMAAGRPLVVDADGLNLLAEAPVLRDDWVLTPHPGEAARLLDSCVAEVQADRFAAVEQLQRRYGGVAVLKGAGTLIASGGDRRPAVCSDGNPGMASGGMGDVLTGVVAGWLAQGQPLAAAAELAVSLHSAAADKAARAGRRGLLAMDLMSHVRRLANPDNE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.2. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.3. A protein with NADPHX epimerase activity, NADHX epimerase activity functions is required to enable nicotinamide nucleotide metabolic process.4. The designed protein should have a high specificity for K(+), (6S)-NADPHX binding, with minimal off-target effects.5. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.. The designed protein sequence is ",
    "response#1": "MRVTDKNTQYNHIPTIVLMENAGSRIADYLINNYPDKKKVSIYSGTGGNGGDGFVIARHLLNHGYKVRLFMLSKPENIKNKDSQLNYQSIKIISQTDKNLKLSTITDSSQVSPDNSDIIIDAILGTGVNGKLRQPVSSVIDTINYSPAIVISVDVPSGMNPENGKVTDKCVVAHETLTLHKMKTGLQKAPESYVGKISVLDIGIPRVSEVEKIVRERQYDGYKGTYGRVLLLTGSRQYPGAGLISTAAALYSGAGLVQLSSDAEVMKMAVSRYPEILTALADQQLDSMLDAASAVLFGCGRGWNDTTRLELEEVLVKSRVPVVLDADGINCLASTPELLTKVSCPVILTPHLGEMQRLLENETEKDPVVGAIQFARKYHAIVVLKGPLTMVTDGKHVFRNPSGNGAMASAGMGDALAGIVVSLLAQGYAYTDACVLGVYLHGCCGDQIAKNQYTVLASQLIEKIPEVMHN",
    "response#2": "MRMADEFTVASQKITSLDLMERAATQIFNMLHQRLQGAPIPIHVFCGLGNNGGDGLVVSRLLIEHGYDVKTYIVNFSNNRSKDFLENYDRLKSILSEWPIQLKSDDNFPVIKKDDMVIDAIFGIGLNRPLLPWVISLIKHINASRCFVISIDVPSGLYADKVPDDPEGVIYAATTVTFQLPKLIFFLPETGQYSRDLEVIDIGLDRDFITFDVATRLAPVRPTDGHKGVFGHLLTVGGSRGKMGAGVLAASGGLRSGAGLVTVACPASCQAILDCRLDEAMSIGLPEGEGHLVAESFPCLETHFAGKQAVALGPGIGQREGVAALVRKVVSDCPLPLVIDADGLNALAEDPSPLRNRAALSTVLTPHPGEMARLLNISVPEVESDRLRHARWFAAEFGVALVLKGARSIVASPDGRCWINASGNPVMAVGGSGDVLTGLIGGLLAQDFDPVTAAVLGVFVHGAAADRIAELHGKSGLYASEVAAAIPDIWFEKDSHKGQNGSVLIIGSNPDYIGAVVFAAQAALSQRMDLVYIIAPEASSNIIKQYNPEFIVRGVPGDTLTMDAYDAIMQLEKRVDSILIGSGSGTDEKTGELFNNILSSTEKPLVIDADALKLISPEMLSQNMVVTPHAHEFKQLFGMEVPEKIDERVEMLMNLSGDYGCQILLKGVTDVITSPEDYKLNRTGNQGMTKGGTGDLLAGLTLALSTKNTLFESAFLSAFILGSACDKLLEEKGLDYGIRDILKKY",
    "response#3": "MRMADEFTVASQKITSLDLMERAATQIFNMLHQRLQGAPIPIHVFCGLGNNGGDGLVVSRLLIEHGYDVKTYIVNFSNNRSKDFLENYDRLKSILSEWPIQLKSDDNFPVIKKDDMVIDAIFGIGLNRPLLPWVISLIKHINASRCFVISIDVPSGLYADKVPDDPEGVIYAATTVTFQLPKLIFFLPETGQYSRDLEVIDIGLDRDFIEENDIRERLIHRAAFSHKGTFGHAMLVAGAPETMGAALLTSRACLYAGAGLTSACIPATGLTALNASLPEVMAVVRSEQGLPTSVDWEKYQAIGIGPGLGTGPGSLSLLRVVLKNFHKPIVLDADALNLIAHNYELIQQVPEHSVLTPHMKEFDRLFGDHQDWWSRIETGIDRATTLGCTIVLKNRYTIIFTPQGMCLFNPTGTPAMATGGSGDALTGIITSFLAQGYEPHEAAFIGTYVHGAAGERVRGYVATPSQLIDELPLLMKELAE"
  },
  {
    "reference": "MVFKKIKTQIDHALKQLNLPTDVEYLIQQTKNIQFGDFSSNVAMVLSKRQNKNPQEIAKQIIEQLNPNEFEKITFSKPGFINFFLSNQDKLVVLKRLQETNYSVEKLPKEEQESINIEFVSANPTGFLHLGHVRNAYTGDVLSNILRAVGHNVTKEYWINDLGNQVSLFALSTIIRYLQELGINKYELPDDSYHGKEPIFVAEEMIKDFGNKYQDIKIEDNKIVDSKIANELTQYCTNKMLHFIKQDLESIGVKMDVWTSEKVVYQSNTLTELLNNQLKDHIYEQDGAVWLRTTDGGDDKDRVIIKENKQPTYYGTDIANHYLKHKRGFDRLINVWGADHFGHILRTAYAAELTGIKKGKFVVVLIEMVKLLKDNKEIKFSKRLGNAISIPDMLEFLSKDASRWFMLNQSWTSGIRIDVDLTNKKDSSNPVYYVQYAHARIHKLLTKAEHIDLNKVNLSLLNSDVERTMVNYLASFEHYVHNVATTYEVNKLLNFVYTLTQSFHSWYNSHEILNQKDEIKQTRLLLAKAIKNLINYLLSLFGIEAVEQM",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit arginine-tRNA ligase activity. The designed protein sequence is ",
    "response#1": "IQYAHARACSLMRNAPTLLPIEDVDFSRVSSAPEIALIKLLVRWPNIVESSAVNHEPHRIAFYLLEVAEAFHVLWGHGSRSVDMRFIVEGDIATTSARIYLVKVVALVISLGLSIFSIAPMEEC",
    "response#2": "VQYVHARCCGILDEADGLDPEATIAPLDTDAERDLLRTVARLPGVIESAADDHQPHKIATYTRRLADRFNTFYRECPVTTADDAEVRRARLALVAAARHAMANALDILGVEAPESA",
    "response#3": "VQYVHARCCGILDEADGLDPEATIAPLDTDAERDLLRTVARLPGVIESAADDHQPHKIATYTRRLADRFNTFYRECPVTTADDAEVRRARLALVAAARHAMANALDILGVEAPESS"
  },
  {
    "reference": "MNAAPITQDLLTIPRKLPETAGRNLVGLTREQLHEALIQAGTPERQARMRVGQIWQWIYHWGVRDFAQMTNLAKDYRALLAENFEIALPEIVTRQISADGTRKYLLRISGGHEVETVYIPEENRGTLCISSQVGCTLTCSFCHTGTQKLVRNLTAGEIVGQVMVARDDLGEWPKPGAPKDETRLVSNVVLMGMGEPLYNFDNVRDAMKVVMDGEGISLSRRRITLSTSGIVPEIAKTAEEIGCLLAVSFHATTDETRDKLVPVNRKWNIETLLNALREYPRLSNSERITFEYVMLDGVNDSDEDARRLVRLIRGIPAKVNLIPFNEWPGSPYRRSGWERIEAFADIVHKAGYASPIRTPRGEDIMAACGQLKSATERGRKTAAQIAAEARA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.2. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs3. The ideal protein would be one that plays a critical role in rRNA base methylation through rRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding within cytoplasm.4. The designed protein should have a binding site that is specific to the target S-adenosyl-L-methionine ligand. The designed protein sequence is ",
    "response#1": "LSVCVTTQVGCNIGCSFCASGLLSKNRDLSAGEIVEQIVKVQLHLDQAQQDERVTHIVVMGIGEPFDNYENLLDFLTVVKDPKGLAVGPRHITVSTSGLADKIRQFADDDPGVNLAVSLHAPNNEIRTRIMKINRAIPIEQLMQAIDYYLDKLNRRITLEYILLKDVNDRQEHALELAELIGDRRPLVNVNLIPYNPVDEHSQYHRSEQESIRMFYQ",
    "response#2": "LSVCVTTQVGCNIGCSFCASGLLSKNRDLSAGEIVEQIVKVQLHLDQAQQDERVTHIVVMGIGEPFDNYENLLDFLTVVKDPKGLAVGPRHITVSTSGLADKIRQFADDDPGVNLAVSLHAPNNEIRTRIMKINRAIPIEQLMQAIDYYLDKLNRRITLEYILLKDVNDRQEHALELAELIGDRRPLVNVNLIPYNPVDEHSQYHRSEQESIRMFYG",
    "response#3": "DTERGTLCVSSQVGCTLNCSFCHTGTQRLVRNLTAGEIVGQVLAARDKLGDWPGGVRPTDGVVPTGDRCVTNIVMMGMGEPLYNFEAVRDALLIVSDGDGIGISKRRITLSTSGVVPMIERAGNEIGCMLAISLHAVRDDLRNELVPLNRKYPIKELLNACRNYPGASNAKRITFEYVMLKDVNDSIEDAKALVRLLKGIPAKINLIPFNPWPGSKYECSDWEQIEKFSQVVFDAGYASPVRTPRGRS"
  },
  {
    "reference": "MKLTSLRVSLLALGLVTSGFAAAETYTVDRYQDDSEKGSLRWAIEQSNANSAQENQILIQAVGKAPYVIKVDKPLPPIKSSVKIIGTEWDKTGEFIAIDGSNYIKGEGEKACPGANPGQYGTNVRTMTLPGLVLQDVNGVTLKGLDVHRFCIGVLVNRSSNNLIQHNRISNNYGGAGVMITGDDGKGNPTSTTTNNNKVLDNVFIDNGDGLELTRGAAFNLIANNLFTSTKANPEPSQGIEILWGNDNAVVGNKFENYSDGLQINWGKRNYIAYNELTNNSLGFNLTGDGNIFDSNKVHGNRIGIAIRSEKDANARITLTKNQIWDNGKDIKRCEAGGSCVPNQRLGAIVFGVPALEHEGFVGSRGGGVVIEPAKLQKTCTQPNQQNCNAIPNQGIQAPKLTVSKKQLTVEVKGTPNQRYNVEFFGNRNASSSEAEQYLGSIVVVTDHQGLAKANWAPKVSMPSVTANVTDHLGATSELSSAVKMR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Converts dehydroshikimate to protocatechuate..2. The protein design should take into account the physiological conditions of the 3,4-dihydroxybenzoate from 3-dehydroquinate: step 2/2, Aromatic compound metabolism pathway.3. A protein with 3-dehydroshikimate dehydratase activity is needed to enhance 3,4-dihydroxybenzoate biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "MQGMTALILGAGGVSRAIAWGLKQRHCDVVIASRTFERAEILASELGCR",
    "response#2": "MQGMTALILGAGGVSRAIAWGLKQRHCDVVIASRTFERAEILASELGCQ",
    "response#3": "MQGMTALILGAGGVSRAIAWGLKQRHCDVVIASRTFERAEILASELGCS"
  },
  {
    "reference": "MKMIIGRKIGMTRVFVGNDSVPVTVIKAGPCVVVQKKTVEKDGYNAVQLGFEKAKKVNKPLAGHFKKFGVEPMKILREFRVENPDEYEPGQVIKVDVFEKGEYVDVTGWTKGRGFAGAMKRWGFSGGPKSHGSKFHRELGSVGQHTEPAKIWKGKKMPGRYGNERVTVRNLQVVDIDPENDLLVVKGGVPGARGGLVLIRSAKAPKK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have rRNA binding, structural constituent of ribosome and be active in ribosome, ribonucleoprotein complex.2. The protein must exhibit the following characteristics: One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.. The designed protein sequence is ",
    "response#1": "MTGF",
    "response#2": "MSGR",
    "response#3": "EITQL"
  },
  {
    "reference": "MDKSIPIKAIPVACVRPDLVDDVTKNTSTIPTMVSPVLTNMPSATSPLLMVPPLRTIWPSNKEWYDGDAGPSSTGPIKREASDNTNDTAHNTFAPPPEMVIPLITIRPSDDSSNYSCDAGAGPSTGPVKRGRGRPKGSKNSTPTEPKKPKVYDPNSLKVTSRGNFDSEITEAETETGNQEIVDSVMMRFDAVRRRLCQINHPEDILTTASGNCTKMGVKTNTRRRIGAVPGIHVGDIFYYWGEMCLVGLHKSNYGGIDFFTAAESAVEGHAAMCVVTAGQYDGETEGLDTLIYSGQGGTDVYGNARDQEMKGGNLALEASVSKGNDVRVVRGVIHPHENNQKIYIYDGMYLVSKFWTVTGKSGFKEFRFKLVRKPNQPPAYAIWKTVENLRNHDLIDSRQGFILEDLSFGAELLRVPLVNEVDEDDKTIPEDFDYIPSQCHSGMMTHEFHFDRQSLGCQNCRHQPCMHQNCTCVQRNGDLLPYHNNILVCRKPLIYECGGSCPCPDHCPTRLVQTGLKLHLEVFKTRNCGWGLRSWDPIRAGTFICEFAGLRKTKEEVEEDDDYLFDTSKIYQRFRWNYEPELLLEDSWEQVSEFINLPTQVLISAKEKGNVGRFMNHSCSPNVFWQPIEYENRGDVYLLIGLFAMKHIPPMTELTYDYGVSCVERSEEDEVLLYKGKKTCLCGSVKCRGSFT",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a specific function within the nucleus.2. The protein contains novel Post-SET, SET domains that confer a unique function or activity.3. The A.T hook domain within the designed protein should exhibit high affinity and specificity towards its target DNA sequence. The designed protein sequence is ",
    "response#1": "SGEWYFEIKIEKGGGDDGAHVRIGVSRREAPLDAPVGYDAYSYGLRDLGGQKVHMSRPRNFMDSFGTGDIIGLHISLPKPSFAQHTTLPSCHDRIPIRYKGQLYFEQPDYVPSKMMDELMIPSKHNRYIDLPYIPGSFIKVYKNGSYMGTAFENLLDFNPPNSINSNHYSFDDGSLGYYPSISMYGGGIARFQFGPQR",
    "response#2": "SGEWYFEIKIEKGGGDDGAHVRIGVSRREAPLDAPVGYDAYSYGLRDLGGQKVHMSRPRNFMDSFGTGDIIGLHISLPKPSFAQHTTLPSCHDRIPIRYKGQLYFEQPDYVPSKMMDELMIPSKHNRYIDLPYIPGSFIKVYKNGSYMGTAFENLLDFNPPNSINSNHYSFDDGSLGYYPSISMYGGGIARFQFGPQR",
    "response#3": "SGEWYFEIKIEKGGGDDGAHVRIGVSRREAPLDAPVGYDAYSYGLRDLGGQKVHMSRPRNFMDSFGTGDIIGLHISLPKPSFAQHTTLPSCHDRIPIRYKGQLYFEQPDYVPSKMMDELMIPSKHNRYIDLPYIPGSFIKVYKNGSYMGTAFENLLDFNPPNSINSNHYSFDDGSLGYYPSISMYGGGIARFQFGPQCL"
  },
  {
    "reference": "MQDAKTYLSIAPVLATLWFGFLVGSLIEINRFFPNALILPSL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The designed protein should have a specific function within the photosystem I.3. For general function, the protein need meet that May help in the organization of the PsaE and PsaF subunits. The designed protein sequence is ",
    "response#1": "EPQALNNIAVIYHYQAVKASEINDLETAQALFHEC",
    "response#2": "MPNGNPGR",
    "response#3": "GPQALNNIAVIYHYQAVKASEINDLETAQALFHEL"
  },
  {
    "reference": "MRAVSDAPKSPVKADIASLSQEALTQFVVTTLGEKSFRAKQLYKWIHQRGATSFEEMTDLSKDLRAKLTEHAHIATLTKDLEQRSIDGTIKYRWKTWDGKLIESVYMPSPERKTLCVSTQVGCAMGCRFCATATLGLMRNLSPAEIVAQVHAVNREVRGTDTKLLRPLTNLVFMGMGEPLHNFENLKTSLSILQSEDGPNFSSRHITVSTVGLVPMIERFGQETDVKLAISLNASSDETRDGIMPINKKWKIADLMEACRKFPLKQGRRITFEYVLMGGVNDADDDAHRLATLLVGVPAKVNLIQYNENPGLGYHTTVDNRAERFREILERAGVAAFIRANRGRDIAAACGQLANTEKERTFTPLRPLRAAVADP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs; m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.2. The designed protein should be able to compete with endogenous proteins or inhibitors for S-adenosyl-L-methionine, [4Fe-4S] cluster binding.3. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity.4. The stability of the protein-[4Fe-4S] cluster complex should be optimized to maximize enzymatic activity.5. The designed protein should be involved in rRNA base methylation by localizing to cytoplasm and exhibiting metal ion binding, rRNA (adenine-C2-)-methyltransferase activity, tRNA binding, 4 iron, 4 sulfur cluster binding, tRNA (adenine-C2-)-methyltransferase activity. The designed protein sequence is ",
    "response#1": "SQVGCTLSCSFCHTGTMKFARNLNLAEILSQVLYVKHDLFDWQKRTEEKRITNIVFMGMGEPLFNYENVIKSVNILSDPEGLALSKRKITLSTSGVVPRLVDFKYEKDINLAISLHAVFDKLRNTLVPINKKWPLEKLINVLKEYNSYRENKRITFEYIMLKDVNDSLEDAKEEPTTVVDGVAEPLDLVGLSREDLGTALANVGVPQKQVRMRVSQLWHWLYMRGVNDFDRMSNISKEMRALLKANFTIGWPEIADEQISNDGTRKWLLRFPSRGAGRPVEVETVYIPEEGRGTLCVSSQVGCTLTCSFCHTGTQKLVRNLTSGEILSQLILARHRLGDFPDVEAAPGTMVPSEGRKVSNIVMMGMGEPLYNFENVKQALLIASDGEGLSISRRRITLSTSGVVPEIRRAGEEIGVMLAISLHAVRDDLRDELVPINKKYPLKDLLQACRDYPGLSNARRITFEYVMLKGVNDSLEDARDLVKLLKGIPAKINLIPFNPWPGSAYECSDWEQIERFADFVNHAGYASPIRTPRGRDILAACGQLKSESERLRKSARYRQSEEG",
    "response#2": "EEGRGTLCVSSQVGCTLTCSFCHTGTQKLVRNLTSGEILSQLILARHRLGDFPDVEAAPGTMVPSEGRKVSNIVMMGMGEPLYNFENVKQALLIASDGEGLSISRRRITLSTSGVVPEIRRAGEEIGVMLAISLHAVRDDLRDELVPINKKYPLKDLLQACRDYPGLSNARRITFEYVMLKGVNDSLEDARDLVKLLKGIPAKINLIPFNPWPGSAYECSDWEQIERFADFVNHAGYASPIRTPRGRDIDAACGQLAGQVQDKTRRAHRYMAVKR",
    "response#3": "EEGRGTLCISSQVGCTLTCSFCHTGTQRLVRNLTAEEILSQLLLARDRLGDFPDREAPQGTIMPAEGRKVSNIVMMGMGEPLYNFDAVKQALLIATDGDGLSLSKRRVTLSTSGVVPEIFRTGEEIGVMLAISLHAVRDDLRDILVPINKKYPLKELIDACKAYPGLSNARRITFEYVMLKDVNDSLEDAKGLIKLLKGVPAKINLIPFNPWPGTNYQCSDWEQIEKFADFINSAGYASPIRTPRGRDIDAACGQLVGQVEDRTRRAARYRLQQPGAAAA"
  },
  {
    "reference": "MGSCCRGLHPLSVLVQAVVLAEALALGTLPDFLPCELKPHGVVNCNWLFLKSVPRFSAAAPHSNITSLSLLSNRIHHLHNSDFIHLPNLRHLNLKWNCPPTGLSPMHFPCHMTIEPKTFLAMPTLEDLNLSYNGITTVPALPSSLVNLTLSHTNILVLDSTSLAGLRALRVLFMDGNCYYKNPCTQAVEVAPGALLGLSNLTHLSLKYNNLTAVPRGLPPSLEYLLLSYNHIIKLESGDLANLTALRVLDVGGNCRRCDHARNPCVECLTKSLQLHPDTFSHLSHLEGLVLKDSSLKALEPRWFQGLENLTVLDLSENFLYDCITKTHAFQGLTRLRKLNLSFNYRKGVSFAHLHLAPSFRNLISLQELNMNGIFFRSLSEYTLRSLFHLPLLQTLHLQMNFINQANLSIFGAFPRLSFVDLSDNRISGTPTLVKALGEADDQNRVWLGPEDLAPVPLGNPRFEDFMFSCKNIPFTLDLSGNNLVTLRSEMFTSLSHLRCLILSHNCIAQAVNGSQFQPLTSLQVLDLSHNKLDLYHGHSFTELPELTTLDLSYNSQPFGMQGIGHNLSFVSRLHKLRHLSLAHNGINSRVNPRLCSSSLQTLDFSGNTVSSMWAEGDLYLHFFQGLRNLHKLDLSQNRLHILLPQNLDSLPKPLTHLSLRDNYLAFFNWSSLSLLPNLLNLDLAGNQLKALTNGTLPNGTQLQRLDVSGNSITFVAQGFFALATDLRELNLSDNILRTIESSWFGTVAGNLKVLDVRANPLHCTCGA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein have TIR domains with specific sequence motifs that are crucial for its activity.2. The protein should be optimized for stability and expression in apical plasma membrane, basolateral plasma membrane where it will participate in positive regulation of interleukin-12 production, positive regulation of interleukin-18 production. The designed protein sequence is ",
    "response#1": "NLNCIVNEGRKMMCQWDPGRDTYLETNFTLKSEWATEKFDDCKMKPDTPNSCLVDYSPVYFVNIEVWVEAENALGNVTSDHINFDC",
    "response#2": "KDFDAFVSYAKWSSFPSEATSSLSEEHLALSLFPDVLENKYGYSLCLLERDVAPGGVYAEDIVSIIKRSRRGIFILSPNYVNGPSIFELQAAVNLALDDQTLKLILIKFCYFQEPESLPHLVKKALRVLPTVTWRGLKSVPPNSRFWAKMRYHC",
    "response#3": "TPRHVSVRNQTGDSVSVEWTASQLSTCPGVLTQYVVRCEAEDGAWESEWLVPPTKTQVTLDGLRSRVMYKVQVRADTARLPGAWSHC"
  },
  {
    "reference": "MIRRKFSRVFSKRTDMHNIIRLLFRNPFFFLFCLFIPFDNTSLQSIGGIMTASPSALILLPGLFVSILSKGLKVNKNILLCFFGVLLISFLYYFYWVFYFPELDPIFILDRGSRYFLLYVFYFLALYYSLRQNIKDIRAGAALIIIVVIFSVLLNYLDPAIINNKSIIQYNDFISPERLRGFSLEASVFGYQIVCSILLLAVLLNWSTFFLVTVTIVIAILTTSKGAALSFLICICFYFSLKGKLMFRVLLSLCSIVISYIIFKYYFLDALASDIDTYSSVATRGTMFIVGLKIFLFNPLGVGFFGYLPSIYDFTSGVIDFIKSHFPFLNFDEVYTYTIPGEYKTVGTKSLILDLLIIYGVFFLIPFIYFIKKILKEFDAQSERNSYFLLLFIIFSNMFFISHLGSYFTPFCIAFLIILSKNRAENDIN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. The designed protein should be expressed specifically in the plasma membrane3. For general function, the protein need meet that May function as a transporter. The designed protein sequence is ",
    "response#1": "STTPPWWAIPPAWSLGAEIQAYFLLPILLTYKMLGLSVFWISYIIYSLANLNIIHSDYFGYRLIPGVIFMFLSGAYLQKIVSGKASRLEMLSLIIIYIISLFWLVFFIIIKGKYGAYTRETLLGLLVGIPLVYTLLKIRRKFYFNDLFGKLSYGIFLSHFLSFWILEFVNLTQNIISMIFLSLIISASVSYLL",
    "response#2": "STTPPWWAIPPAWSLGAEIQAYFLLPILLTYKMLGLSVFWISYIIYSLANLNIIHSDYFGYRLIPGVIFMFLSGAYLQKIVSGKASRLEMLSLIIIYIISLFWLVFFIIIKGKYGAYTRETLLGLLVGIPLVYTLLKIRRKFYFNDLFGKLSYGIFLSHFLSFWILEFVNLTQNIISMIFLSLIISASVSYLF",
    "response#3": "STTPPWWAIPPAWSLGAEIQAYFLLPILLTYKMLGLSVFWISYIIYSLANLNIIHSDYFGYRLIPGVIFMFLSGAYLQKIVSGKASRLEMLSLIIIYIISLFWLVFFIIIKGKYGAYTRETLLGLLVGIPLVYTLLKIRRKFYFNDLFGKLSYGIFLSHFLSFWILEFVNLTQNIISMIFLSLIISASVSYLF"
  },
  {
    "reference": "MTDEFTTDIYYDDDADVSTLDDETVAVLGYGSQGHAHALNLHESGIDVVVGLREDSSSRSAAEADGLTVTTPADAVKQASYVSVLVPDTVQADVYENAIEPNLESGQTLQFAHGLNIHYNQIQPPEGVDVTMVAPKSPGHLVRRNYENDEGTPGLLAVYQDETGEADERALAYAKAIGCTRAGVIETTFREEVESDLFGEQAVLCGGVTSLVKHGYETLVDAGYSPEIAYFECLNELKLIVDLMYEGGHSEMWDSVSDTAEYGGLSRGDRIVDENVRENMEETLEEIQNGDFTREWIVENQAGRPSYTQLRQAEKDHEIEEVGERLRDLFAWADEGTETDDEDESVRVQADD",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should interact with enzymes and substrates within the L-valine biosynthesis, Amino-acid biosynthesis pathway to enhance or regulate its activity.2. The protein should exhibit Acidic residues compositional bias.3. The protein must exhibit the following characteristics: In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB); Involved in the biosynthesis of branched-chain amino acids (BCAA).4. The designed protein should contain one or more KARI N-terminal Rossmann domains that are essential for its function.5. The designed protein should have NADP binding, ketol-acid reductoisomerase activity, isomerase activity to facilitate valine biosynthetic process, isoleucine biosynthetic process.6. The protein's active site must be carefully engineered to ensure that the amino acids involved in (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-   ethyl-2-hydroxy-3-oxobutanoate + H(+) + NADPH are properly positioned for optimal catalysis.7. The presence of Mg(2+) is necessary for the protein to function in the desired environment.8. The designed protein should have a binding site that is specific to the target NADP(+), substrate ligand. The designed protein sequence is ",
    "response#1": "MATVYYDKDADPRALRGKTIAVIGYGSQGHAHAQNLRDSGHDVVVGLQAGSKSWAKAEEDGFSVRETADAARHADIVALLVPDQHHREAYAKIAPFMGKGRTLLVAHAFSVHFGEVKPDAQADVVMIAPKAPGHRMREVFKEGKGVPALLAVYQDVSGKAEQTALAYAQAVGATRAGVIKDGLTVPKGAVSVTFREETETDLFGEQAVLCGGTASLIKAGFETLVDAGYAPEMAYLEVCHELKLIVDLIYEGGLTNMRQYVSNTAQYGDMTRGPRVIGPEAYEAMEEILAEIQSGEFAKEWMLENMVNRPVFNALTKADEEHLLEETGKEVRAMMPQTFAEEVETDWFGEQVILCGGISELMKASFDTLVDAGYQPEVAYFECVNEMKLIVDLIYEGGLSYMRYSVSDTAEYGDYTVGPRIIDARVRAEMKKVLSDIRDGTHAKKWIAEYRNGAPNLYAQRAKEQEQPLEQVGRRLREMMPWLGID",
    "response#2": "MRVYYDRDCDVNLIKEKKVAILGYGSQGHAHALNLRDSGAKNLVIALREGSPSAKKAEAEGLQVMGIAEAAAWCDVIMFTMPDELQAETYKKYVHDNIKPGAAIAFAHGLNVHFGLIEPKEGVDVIMMAPKGPGHTVRGEYTKGGGVPCLVAVHNDASGSALETALSYCSAIGGGRSGIIELADRKVAVIGYGSQGHAHALNLRDSGVDVRVGLPADSRSRARAEEEGLRVLTPAEASAEADIIMLLTPDTTHRTIYAESIAPHLTAGKALAFGHGFNIRYGLIEPPAGVDVFMVAPKGPGHLVRRVFEEGKGVPVLVAVEADASGNALAVALAYAKGIGGTRADAFKDETETDLFGEQAVLCGGAVELVKMGFETLTEAGYAPEMAYFECLHELKLIVDLMYEGGIADMNYSISNNAEYGEYVTGPEVINEQSREAMRNALKRIQSGEYAKMFITEGATNYPSMTARRRNNADHEIEKTGAKLRSMMPTFREETETDLFGEQAVLCGGTASLIKAGFETLVDAGYAPEMAYLEVCHELKLIVDLIYEGGLTNMRQYVSNTAQYGDMTRGPRVIGPEAYEAMEEILAEIQSGEFAKEWMLENMVNRPVFNALTKADEEHLLEETGKEVRAMMPQLCATVKG",
    "response#3": "MATIYYDKDADLKALKGKTVAIIGYGIQGRGQALNLRDSGVKVIVAQRPGGPNFDLAKQDGFTPLSAAEATKKADVVILLAQDMLQGDIYREAIAPNLRPGQTLGFSHGFAVLYGLIKPPKNVDVVMVAPKGPGSLVRSQYLEGKGVPALVAVYQDASGKAKATALAWAKGIGATRAGVLEDTIAYDGKISEQEYFDNGVLMIAMVKAGVELAFDAMVASGIYEESAYYESLHELPLIANTIARKRLYEMNVVISDTAEYGNYLFSNVATPILAKEIIPNLQKGDLGEPTPAVEVDNITLRDVNDAIRNHPVELIGQELRGYMTDIFG"
  },
  {
    "reference": "MAQRFITLILLLCSILLAPHSAQASLFGDNSSFGQKSSQSRFIPVDQAFAFDFRQQGDQLALSWQIHPDYYLYRQQIKIVPNNATFGAFTLPEGIAHRDEFYGEVAIFKQQLTLKIPITQAGERASVSVTYQGCAEAGFCYPPETRVVPLSAITAGSSAVTAPPIAAPATPAPAELPFSPLWALLIGIGIAFTPCVLPMYPLISAIILGREKPHSQRRILLLAIVYVQGMALTYTLLGLVVAAAGLQFQAALQHPYVLIGLSALFVLLALSMFGLYSLQLPSSLQTRLVQWSSSQRGGSLAGVFAMGALAGLICSPCTTAPLSAILLYIAQSGNMLAGGGTLYLYALGMGIPLVIVTLFGNKLLPRSGPWMQYVKEAFGFVILALPVFLLERVLGDVWGLRLWSLLAIAFFGWAFILSLKAHSGWARAFQLLLLAALLIAARPLQDWAFGSPTQQSEIKHLAFKQVADLPQLQAALAQAKGKPVMLDLYADWCVACKEFEKYTFSDEQVQRQLADTVLLQADVTANSAEHAALLKELNVLGLPTILFFDAPGNEIPAARVTGFMNASGFLQHLQNLPR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a low Km value for [protein]-dithiol + NADP(+) = [protein]-disulfide + H(+) +   NADPH.2. The presence of Thioredoxin domains is critical for the interaction of the designed protein with its intended targets.3. The protein must exhibit the following characteristics: Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes; This transfer involves a cascade of disulfide bond formation and reduction steps..4. Incorporate a signal peptide in the protein design.5. The designed protein should have at least Helical transmembrane domains to ensure proper localization.6. The ideal protein would be one that plays a critical role in cytochrome complex assembly through electron transfer activity, protein-disulfide reductase (NAD(P)) activity within plasma membrane. The designed protein sequence is ",
    "response#1": "MLDLYADWCVACKEFETHTFSDVAVQKAFTDVLLLQVDMTKNSEANRELMQKLHVIGLPTLIFFNQQGQEIEGSRITGFMQASPFVDWVSFPITVFTDITCGYCHKLHEQWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTGRALLLTFIYVQGMALTYTALGLVVAAAGLQFQAALQHPYVLIGLALVFTLLALSMFGLFTLQLPSSLQTRLTLMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYALGMGLPLILITVFGNRLLPKSGPWMEHVKTAFGFVILALPVFLLERVWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTGRALLLTFIYVQGMALTYTALGLVVAAAGLQFQAALQHPYVLIGLALVFTLLALSMFGLFTLQLPSSLQTRLTLMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYALGMGLPLILITVFGNRLLPKSGPWMEHVKTAFGFVILALPVFLLERVYLLPVALYVILVSVRPLQDWAFGATTAQTQAHLNFKPITTVDALNQALAEAKGKPIMLDLYADWCVACKEFEKYTFSDPQVQQTLGDTVLLQANVTANNAQDVALLRHLNVLGLPTILFFDAQGHEHPNARVTGFMDATTFSAHLRDRQS",
    "response#2": "MLDLYADWCVACKEFETHTFSDVAVQKAFTDVLLLQVDMTKNSEANRELMQKLHVIGLPTLIFFNQQGQEIEGSRITGFMQASPFVDWFQDAALAEARQAGKPVLLDWYADWCISCKVIERQVLTDPTVQARLPAYRLLRFDITESNPAQRGLLDRYNLFGPPAILFFAPGGDEWSDLRVIGEIDAAGLAERLVRP",
    "response#3": "MLDLYADWCVACKEFETHTFSDVAVQKAFTDVLLLQVDMTKNSEANRELMQKLHVIGLPTLIFFNQQGQEIEGSRITGFMQASPFVDWNH"
  },
  {
    "reference": "MSPSLHPLLDNGITKGDPNFPGGNLYCKCSSDKVVVKLASNVAHNHACGCSKCWKPAGSLFSIVGVVPRDAVSVTEHAEKLSIVDASAAIQRYACKGCGVHMFGRIEKDHPFKGLDFVHAELSDQKGWQEPQFAGFVSSIIEQGFHPKGMEEVRSKFHSLGLETYDALSPALMDLIATFTAQRAGVLSANL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must have high specificity towards S-(hydroxymethyl)glutathione synthase activity.2. A protein that is capable of catalyzing reactions within the formate from formaldehyde (glutathione route): step 1/3 pathway with high efficiency and specificity.3. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity.4. The protein should have a low Km value for S-(hydroxymethyl)glutathione = formaldehyde + glutathione. The designed protein sequence is ",
    "response#1": "FAGGTLVCACTSKPVKVKVKGQIAHNHACGCTKCWKPEGAIFSVVAVAGTGDVTVTENGDKLKVVDASALIQRHACTGCGVHMHGPVERDHAFKGLTFIHPERFVEDGWSPPGFTAFVSSIIESGVDPKRMDGIRAQLRTIGLEPYV",
    "response#2": "FAGGTLVCACTSKPVKVKVKGQIAHNHACGCTKCWKPEGAIFSVVAVAGTGDVTVTENGDKLKVVDASALIQRHACTGCGVHMHGPVERDHAFKGLTFIHPERFVEDGWSPPGFTAFVSSIIESGVDPKRMDGIRAQLRTIGLEPYV",
    "response#3": "FAGGTLVCACTSKPVKVKVKGQIAHNHACGCTKCWKPEGAIFSVVAVAGTGDVTVTENGDKLKVVDASALIQRHACTGCGVHMHGPVERDHAFKGLTFIHPERFVEDGWSPPGFTAFVSSIIESGVDPKRMDGIRAQLRTIGLEPYR"
  },
  {
    "reference": "MSSLTSRDFALQSDANDSLSSLRQKFHIPQHNGADVMYFTGNSLGLQPVDAKKYIDEELEDWKNLGVEGHVHARRPWMPYHENFTKSLARLVGSKPEEVVAIGTLTNNLHLLMVSFYRPTESRFKILCEAKAFPSDQYALASQARFHGFDPKEAIIEVSPREGEHTIREEDIHEIIEREGDSIAMMMIGGVNYYTGQVFDMNSLTKFARAKGITVGWDLAHGIGNIELKLHEWDVDFAAWCSYKYLNSGPGSIAGIYIHERHHGQRDIPRFEGWWGHDKGSRFAMPDQFEPIPTAEAWQLSNVPVLAMAAHRASLDLFDHVDLAELRARGRKLSNYLREVLLEVAELTGREIEIITPANEAQSGCQVSVLFPGLGKDLFDKLLAAGVIADWREPHVIRMAPVPMYSSFEDIYRFGETLLNVLKEA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively..2. The designed protein should interact with enzymes and substrates within the NAD(+) biosynthesis pathway to enhance or regulate its activity.3. A protein with pyridoxal phosphate binding is needed to enhance L-kynurenine catabolic process, quinolinate biosynthetic process in a specific context.4. The pyridoxal 5'-phosphate binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MELVGAKQNEVAVMGSLTSNLNALLIHFYKPKGKRTKILFEKQAFPSDYYAFLNIVQVFGYDASHLIQIEIPKGETYIKTETILDVFDKYEDEIAIVCLPGIQYYTGQFFDIAKITKHVKTSAPDVVVGWDLAHAVGNVPLSLHDWGVDFAAWCSYKYLNAGPGAIAGIFVNEKYTEQNKPENLIILVGAKQNEVAVMGSLTSNLNALLIHFYKPKGKRTKILFEKQAFPSDYYAFLNIVQVFGYDASHLIQIEIPKGETYIKTETILDVFDKYEDEIAIVCLPGIQYYTGQFFDIAKITKHVKTSAPDVVVGWDLAHAVGNVPLSLHDWGVDFAAWCSYKYLNAGPGAIAGIFVNEKYTEQNKPENLLKNLTESVKTIHKYFPDYFITYFTQKGLDKFMEMVGAQYTCPHGRPISVQLSEKELDEYFK",
    "response#2": "LVGAKQNEVAVMGSLTSNLNALLIHFYKPKGKRTKILFEKQAFPSDYYAFLNIVQVFGYDASHLIQIEIPKGETYIKTETILDVFDKYEDEIAIVCLPGIQYYTGQFFDIAKITKHVKTSAPDVVVGWDLAHAVGNVPLSLHDWGVDFAAWCSYKYLNAGPGAIAGIFVNEKYTEQNKPENLLVGAKQNEVAVMGSLTSNLNALLIHFYKPKGKRTKILFEKQAFPSDYYAFLNIVQVFGYDASHLIQIEIPKGETYIKTETILDVFDKYEDEIAIVCLPGIQYYTGQFFDIAKITKHVKTSAPDVVVGWDLAHAVGNVPLSLHDWGVDFAAWCSYKYLNAGPGAIAGIFVNEKYTEQNKPENLKKI",
    "response#3": "LVGAKQNEVAVMGSLTSNLNALLIHFYKPKGKRTKILFEKQAFPSDYYAFLNIVQVFGYDASHLIQIEIPKGETYIKTETILDVFDKYEDEIAIVCLPGIQYYTGQFFDIAKITKHVKTSAPDVVVGWDLAHAVGNVPLSLHDWGVDFAAWCSYKYLNAGPGAIAGIFVNEKYTEQNKPENEKLLVGAKQNEVAVMGSLTSNLNALLIHFYKPKGKRTKILFEKQAFPSDYYAFLNIVQVFGYDASHLIQIEIPKGETYIKTETILDVFDKYEDEIAIVCLPGIQYYTGQFFDIAKITKHVKTSAPDVVVGWDLAHAVGNVPLSLHDWGVDFAAWCSYKYLNAGPGAIAGIFVNEKYTEQNKPENEKLNIPTFFELIQKFY"
  },
  {
    "reference": "MSAAAMLAPDVLAELARRGVGVSRLCVDSRRVTAGDVFVAAPGAQADGRRFIGEALARGAAAVLYEAAGAPPLPATAVPLLPVAGLAGQLGQIADLVYQRPSAQLCLIGVTGTNGKTSVTHFIAQALAALVRPCALIGTLGNGFPDALDPSPNTTPDAITLHAALARYLAQGARACAMEVSSIGLEQGRVAGARFAVAVLTNLTRDHLEYHGTMEAYAASKRLLFETPGLGAAVLNLDDPFGQELAQRLRGRMRTIGYTLGAAGGADEVLAASDLDLRSGLAFRVNGQPAHASLVGRFNVANLLAALGALQAAGIALEQALTTLPRLRPPAGRMEAQGGSGQPLVVVDYAHSPDALEQALTTLRETARERGGRLVCLFGCGGDRDPGKRPLMGAVAERLADRVVLTSDNPRGEEPGAIVAAIRAGMAGEPETELNRGRAIHSTVARADTRDVILVAGKGHEPYQEIGGVRHPYSDLEQVRAALEQRT",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should include a Meso-diaminopimelate recognition motif motif to ensure proper function.2. The protein contains novel Mur ligase C-terminal, Mur ligase central, Mur ligase N-terminal catalytic domains that confer a unique function or activity.3. The designed protein should be able to compete with endogenous proteins or inhibitors for meso-2,6-diaminoheptanedioate, ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding.4. A protein that can modulate the overall flux of metabolites through the peptidoglycan biosynthesis, Cell wall biogenesis pathway, enhancing or suppressing its activity as needed.5. We need a protein that can perform ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate with high efficiency. The designed protein sequence is ",
    "response#1": "MTLRLDSREVCHGDCFVAISGHQLDGLRFIASAIEKGAVCVLVDASADVTDFSVEVIKVPSLKSKLASIAADFYGHPTDKLSLIGITGTNGKTTITYLLEEILTGLGYAVGVIGTVNYRYTAAGEKAVLPASHTTPDAMHLQELLRRMVDAGISHVIMEVSSHALAQARIGNIQFDVAAFTNLTRDHLDYHADMYEYFETKARLFTHHLKAAGNAVIGYPQGTAEEGGWSGMLALQCRDRGIRALICGAHPEADIRLTGFEADLRGNRMTVATPDGGHSLHSPLVGRFNADNVMVALAVAFALGRSVDDAVAALAECRGVPGRLEQVETGRDFHVVVDYAHKPDAVEAALSTLRPLTAQAGGRLICVIGAGGDRDTGKRPVMGEIAARLADLVIVTDDNPRSEDPAVIRAAVLEGTRDGAGEVREVGDRREAIRVALSSAGPGDIVLVAGKGHETYQEVGSQRLPFSDRQVARERLAALAG",
    "response#2": "MRLRELLAGIPLLGAPPQGAAVVSGLAQDSRRVRPGDLFLAVNGARGHGLAHWPEALRRGAVGVLWDPQTPAGLPQVVLPDTRSQVALLAANFYGNPAARLRLIGITGTNGKTTSAYLLKSVLESLGRRVGLLGTVDYRWGGRRMAAPHTTPESLELQRLFREMAEAGCDTAVMEVSSHALALDRVWGCEFDVGVFTNLTQDHLDYHHTMEEYFAAKARLFQGLEDRAYAVVNADDPYGRRLYEATPARVLRYGLQKGADLRAENLTLHQGGMEFLLVTPWGRQKIHSPLFGSFNVYNLLAAVAAGVALGIPLPQAAAGIEQVATVPGRLEQVETGRDFHVVVDYAHKPDAVEAALSTLRPLTAQAGGRLICVIGAGGDRDTGKRPVMGEIAARLADLVIVTDDNPRSEDPAVIRAAVLEGTRDGAGEVREVGDRREAIRVALSSAGPGDIVLVAGKGHEDYQIIGDRKFHFDDREEAAAALARRQEH",
    "response#3": "MTVTGISLSTQRIHPGDLYAALPGTRAHGASYSAAAVEAGAAAIVTDAAGAEIVAGLDLDVPVLVVPDPRAVLGTLSAHVYGRPSDHLRVIGITGTNGKTTITYLLEEILTGLGYAVGVIGTVNYRYTAAGEKAVLPASHTTPDAMHLQELLRRMVDAGISHVIMEVSSHALAQARIGNIQFDVAAFTNLTRDHLDYHADMYEYFETKARLFTHHLKAAGNAVIGYPQGTAEEGGWSGMLALQCRDRGIRALICGAHPEADIRLTGFEADLRGNRMTVATPDGGHSLHSPLVGRFNADNVMVALAVAVEAGIDPLTAAVGVAEAAVPGRLEQVETGRDFHVVVDYAHKPDAVEAALSTLRPLTAQAGGRLICVIGAGGDRDTGKRPVMGEIAARLADLVIVTDDNPRSEDPAVIRAAVLEGTRDGAGEVREVGDRREAIRVALSSAGPGDIVLVAGKGHEQGQDIQGVVHPFDDRQVLRELIAAREDR"
  },
  {
    "reference": "MASASSVSLMLLVAAAMASAASAQLSATFYDTSCPNALSTIKSAVTAAVNSEPRMGASLVRLHFHDCFVQGCDASVLLSGQEQNAGPNAGSLRGFNVVDNIKTQVEAICSQTVSCADILAVAARDSVVALGGPSWTVLLGRRDSTTANESQANTDLPAPSSSLAELIGNFSRKGLDVTDMVALSGAHTIGQAQCQNFRDRLYNETNIDSSFATALKANCPRPTGSGDSNLAPLDTTTPNAFDSAYYTNLLSNKGLLHSDQVLFNGGSTDNTVRNFSSNTAAFNSAFTAAMVKMGNISPLTGTQGQIRLNCSKVN",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.2. The heme b, Ca(2+) binding site should be stable and able to withstand changes in environmental conditions.3. The protein must contain a signal peptide for proper functionality.4. The designed protein should have heme binding to facilitate response to oxidative stress. The designed protein sequence is ",
    "response#1": "ITYSDLWTLAGVVAIEEMGGPKIPWLPGRTDFVDDSKVPPRGRLPDGAQGADHLRFIFYRMGFNDQEIVALAGGHNLGRCHADRSGFQGPWVNNPTRFSNQFFKLLLNMEWKPKTLENGVSQFVYIDPEAEDHEEPLMMLPTDVALRDDPAFRPWVERYAKDKDLFFDHFSKAFAKLIELGIQRDASGKVTNTDNVKY",
    "response#2": "HLEVGFYKHSCPEAEEIVRNAVRRGLARDPGVGAGLIRMHFHDCFVRGCDGSILINSTPGNRAEKDSVANNPSMRGFDVIDDAKAVLEAHCPRTVSCADVVAFAARDGAYLAGGIEYQVPSGRRDGRVSIEAEVLNNNVPAPTDRLRELIESFKRKGLSADDMVTLSGAHTIGRSHCSSFTQRLYNFSGQLGKTDPSIDPAYAEHLKMRCPWPSSDDQMDPTVVPLDPVTPASFDNQYFKNVLAHKVVLTSDQTLLDSPWTAGIVKFNSAVEKAWQLKFAASMIKMGKIEVLTGDEGEIREKCFVVQ",
    "response#3": "SLVFNFYAASCPTAELIVRNTVSSSSSSDPSIPGKLLRLVFHDCFVEGCDASLMLLGNNTEKSDPANRSVGGFSVIESAKRVLEFLCPGTVSCADIIALAARDAVEIVGGPMIEIPTGRRDGMVSVASNVRPNILDTSFTMDEMINRFSSKGLSLFDLVILSGAHTIGAAHCSSFRDRFQEDSKGKLTLIDKTLDNTYADELMKECPLSASPSVTVNNDPETSMVFDNQYYRNLLTNKGLFQSDSALLSDNRTRKFVEDLANDQEFFFESWGQSFLKLTSIGVKTGDEGEIRSSCASIR"
  },
  {
    "reference": "MDISAGMPPDEVGRGGEGITPELARGLFRAGSRVVVLGDAILDVWLSGRCDRLCREAPAPVVDVRQRSYAPGGAANTAVNLAALGARVSMVTAVGDDATGRRLCAELRNRGVGTDHVAVSGGRATVSKRRIVAGDQLLLRFDDGDTGDGDDVEAARLVETLRAALADADALVVCDYANGALGPRVAEALRGLRSELPLLVLDAHDPGRWRALRPDLVTPNAAEAAELLGTDLPDGGDARVEFLDRHRDRLAEATGAAAVAVTLDRDGAVLLTGSQPVHRTWARPAPENYTSGAGDTFVAAATVALAGGLPITTAVELAQAAADIVVHRPGTAVCTAAELEQRFGTYSSAALDHDHLAGVVAEHRAAGRRVVFTNGCFDVLHRGHVAYLNEAKRLGDVLIVAVNADESVRRIKGPDRPVNTVRDRAAVLAALSCVDHVTVFTEDTPVELLRGLRPDIYVKGGDYTPEMLTEAPVVRSYGGQVRVLGYVPDHSTSAVIERIRAGAGTGEPA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The ATP binding site should be stable and able to withstand changes in environmental conditions.2. For general function, the protein need meet that Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7- phosphate at the C-1 position to selectively form D-glycero-beta-D- manno-heptose-1,7-bisphosphate.3. The protein must exhibit phosphotransferase activity, alcohol group as acceptor, heptose 7-phosphate kinase activity, heptose-1-phosphate adenylyltransferase activity.4. The protein contains novel Carbohydrate kinase PfkB domains that confer a unique function or activity.5. A protein that can modulate the overall flux of metabolites through the Nucleotide-sugar biosynthesis pathway, enhancing or suppressing its activity as needed.6. The active site active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "AMSGRTVLCVGDLMRDEFVYGEVARISPEAPAPVIAATRSERHIGGAGNVARNIAALGARCIFAGVVGDDEAGRALRAELAAVPGIEAHLVTDASRPTTRKVRFVSDHFSTHMLRADWEIATPVDAAVENELIGIVEAALPRADIVLLSDYAKGVLTVGVIRATIDGARRLGKRVVVDPKNADFTVYRGASVLTPNQREFAAASRRRATTTPEIVAAAREVMGEGAFEAMLVTRSEHGMTLVPREGEAVHVPAHPVRVRDVSGAGDTVVGVLAVALGAGAGWESALRLANAAAAVAVGKQGTATVSAAELRRLADWRRQGLRIGFTNGCFDILHPGHVRVVTQARAACDRLVVGLNSDASVRRLKGADRPVQTERARAEVLAALEAVDLVVIFDEDTPLELIEAIRPGVLVKGSDYRPEQVVGHDVVTSYGGQVVLVDLLQGFSTTALVERARND",
    "response#2": "AMSGRTVLCVGDLMRDEFVYGEVARISPEAPAPVIAATRSERHIGGAGNVARNIAALGARCIFAGVVGDDEAGRALRAELAAVPGIEAHLVTDASRPTTRKVRFVSDHFSTHMLRADWEIATPVDAAVENELIGIVEAALPRADIVLLSDYAKGVLTVGVIRATIDGARRLGKRVVVDPKNADFTVYRGASVLTPNQREFAAASRRRATTTPEIVAAAREVMGEGAFEAMLVTRSEHGMTLVPREGEAVHVPAHPVRVRDVSGAGDTVVGVLAVALGAGAGWESALRLANAAAAVAVGKQGTATVSAAELRRRLGAEPGGLLTEDQLAYADVERQRGRTIVFTNGVFDILHAGHVHLLRQAKQLGDVLVVGVNSDQSAQRLKGKHRPINSERDRLALVAALDPVDYVALFDEDTPATLIRTLRPHIHVKGGDYADEQLPEAGAVHEVGGRIVIIPLVGSMSTSSVIDRIRSGS",
    "response#3": "AMSGRTVLCVGDLMRDEFVYGEVARISPEAPAPVIAATRSERHIGGAGNVARNIAALGARCIFAGVVGDDEAGRALRAELAAVPGIEAHLVTDASRPTTRKVRFVSDHFSTHMLRADWEIATPVDAAVENELIGIVEAALPRADIVLLSDYAKGVLTVGVIRATIDGARRLGKRVVVDPKNADFTVYRGASVLTPNQREFAAASRRRATTTPEIVAAAREVMGEGAFEAMLVTRSEHGMTLVPREGEAVHVPAHPVRVRDVSGAGDTVVGVLAVALGAGAGWESALRLANAAAAVAVGKQGTATVSAAELRRHLAAGTGDLRSLDETALRDVERQRGRTIVFTNGVFDILHAGHVHLLRQAKQLGDVLVVGVNSDQSAQRLKGKHRPINSERDRLALVAALDPVDYVALFDEDTPATLIRTLRPHIHVKGGDYADEQLPEAGAVHEVGGRIVIIPLVGSMSTSSVIDRIRALG"
  },
  {
    "reference": "MSVLLHLASAQDAVRWLRERVTGTLQTDSRLIQPGDGFIAWPGAATDGRLHVGNALANGAVACLVEQEGVDAFGFDGAHMATMRGLKATTGPIAAAWFDLPTTRLDVMAVTGTNGKTSTAWWLAEALNALAGQADVLTYKGCALIGTLGVGLPGRMESTGLTTPDPVRLQRAFAQFIEEGIGACAIEASSIGLAEHRLAGTRIRTALFTNLTQDHLDYHPSMQAYWEAKRELFDWPGLRTAVINIDDQHGAQLAQELEASQLDIWTVSLRGNARLKARDIRHSDDGLVFTVVEGVHAFMLQTQVIGLYNVSNLLGVIAALRSLGIALEHALWACAQLTPVPGRMERITSAGQPMVAVDYSHTPDALEKALQALQPMARERGGKLWCVFGCGGNRDPGKRPHMGAIAQRFSDEVVVTSDNPRNEEPMSIIEQILKGTTPAQTLRVQADRAQAISETLAAAAAQDVVLIAGKGHEDYQEVRGERHHFSDIEQARAALAQRGGAA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. We need a protein that can perform ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate with high efficiency.2. The presence of Mg(2+) is necessary for the protein to function in the desired environment.3. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..4. The protein design should include a Meso-diaminopimelate recognition motif motif to ensure proper function.5. The protein design must be focused on enhancing the efficiency of regulation of cell shape, cell wall organization.6. The designed protein should be able to compete with endogenous proteins or inhibitors for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding.7. A protein that can modulate the overall flux of metabolites through the Cell wall biogenesis, peptidoglycan biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MTVRGLTADSREVAPGFLFAALPGSAQHGATFAGTALARGAGAILTDAEGARIAAAHLAGSDVALVVAEDPRAALAAAAALYGSPSKDLTMIGITGTNGKTTVATLVWNILTELGHHVGIIGTVTYRYADRIVETERTTPDSHEVHRLLARMLEEGCRCVVMEVSSHALAQDRVVGCEFDVAVFTNLTQDHLDYHHDMEDYFAAKRRLFTDLGLKSAVINADDPYGRRLAEESGGDVLTYGIDSSSDLRARNVETTARGTRFDLVTARGAHSIVVDTPLVGRYNVYNVLAAAAACLAEGVSLSDIQAGLESVSGVP",
    "response#2": "MKLKILLGLLFPFIKTEIRNLVADSRQIKPGDTFVAYPGDTTDGRKFIPQAIAAGANAILWDPRELHDSRKISAGDLFIAVRGYSSDGHNYIGNAIDSGAGIIISEEEIPGNEELFLVAENPRLVLGQAAASFYGHPSQKLKVIGITGTNGKTTVATLVWNILTELGYNCGLIGTIETIIGEQKRPASNTTPESYDLQRLFAEMVSRGCDTVVMEVSSHALEQRRVDGCDFDIAVFTNLSQDHLDFHKTMENYLRAKLMLFRQLKSGGTAVINIDDGYGKRIISESQGVTYGLTDESDFRAKNIRYSTSGTSFHIEGTFKGEAINISSPLMGRFNAYNILAAISAALSLGIPIEVIKEGINSVPAVPGRFEIIKGKEFNVIVDYAHTPEALRDLLIASRALTRKRLIVVFGCGGERDRSKRPLMGEIAARLGDCCILTSDNPRREDPMRILLDVESGIQKVKSRGEYYTFIDRGEAIQKACAEARKGDLVVIAGKGHETYQILKDKTIPFDDRVVAASILREME",
    "response#3": "MLLKELINGLSLDSRSTSKGDLFIALQGLQQHGLEYSQQAIARGAVALAWESSRQVSLLTVSEINFDSRLVKSGSVFVAIRGTQSDGHDYIDIALEKGASAIVCERMPDQIKENITYVQVVDSSQSLGIMAANYYSEPSKKLKLIGITGTNGKTTVATLVWNILTELGENAALLGTVEKKINKKSYESRLTTADPVELAKDMKLMSEEGCNYLVMEVSSHALDQKRTDGFSFAVAAFTNLTLDHLDYHGSMEAYASSKKRLFNKLDSTSWAVINGDDPRGAWMAGSTPAKILTFSFADKGLIKASLVSSSLTGLTLNVDGTTIQTPLIGKFNAYNCVQALLTINLLGIDYKTVLLKISLLKTLSGRFQYFISNGKITAIVDYAHTPDALENVLNTINDIRTKNEELITVVGCGGDRDKTKRPIMAKIASELSTKAIFTSDNPRTEDPETIIEEMEKGVEAQNFKKTVSILDRKQAIKTACQFAQANDIILIAGKGHENYQEINGVRHHFDDVEEAKIAVKKLGLL"
  },
  {
    "reference": "MTKHIFVTGGVASSLGKGLTAASLGRLLKQRGLRVVIQKLDPYINVDPGTLNPFEXGEVFVTDDGGETDLDLGHYERFIDENLTRDSNATSGSIYSAVIAAERRGEYLGRTVQVXPHITDEIQRRIRMLTGEAVDVVITEVGGTVGDIEILPFLEAIRQFRLDAGERNVCYVHVTLVPYIAPSGEHKTKPTQHSVSELRSAGIQPHAXVCRSDEPISDELERKISRLSNVPFEGVVNAPDADSLYEIPLMLHDEGLDDYLCGVLGIDACPPDLTDWKALNQRVADATTXVRIGLIGKYVSLVDAYLSVVESLNHAGIXHGADIDIDWIQAEDVEGLLAENRLRDLDGLVIPGGFGERGIEGKIAAAGYARENDIPCLGLCLGLQCMAVEFARNALGLEGAHSSEFNPATPHPVIDLMESQRDVTDLGGTMRLGAYVAQLRPGSRVAEIYGEEVVSERHRHRYEFNPRYRERFDGTGFVCSGESPDGRLVEFVELDDHPFWIGTQAHPEFKSRPDRPAPLFKAFVEAAMRRTEGRNPQLLQFDEVDVGAAG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must possess metal ion binding, ATP binding to support glutamine metabolic process under certain conditions.2. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen3. The UTP, ATP, Mg(2+), L-glutamine, CTP binding site should be stable and able to withstand changes in environmental conditions.4. The designed protein should interact with enzymes and substrates within the CTP from UDP: step 2/2 pathway to enhance or regulate its activity.5. The presence of CTP synthase N-terminal, Glutamine amidotransferase domains is critical for the interaction of the designed protein with its intended targets.6. The Nucleophile; for glutamine hydrolysis of the protein must be engineered to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+). The designed protein sequence is ",
    "response#1": "MTRYVFVTGGVASSLGKGIISASLAKLLQARGYSVTIQKLDPYLNVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNVPTSQANNVTTGRIYQNVINKERKGDYLGKTVQIIPHITDEIKRNIKLLGTKHKFDVVITEIGGTVGDIESLPYVEAVRQMKWELGNRAMVIHLTLVPYLKASGELKTKPTQHSVKALLETGVQPDVLVLRTEQELSPDIRKKVALFCNVDNKSVIQSIDVPTIYEVPVKMQEEGLDETVLKFFEIKAKKNPDLKKWEEFLERLKNPTEEINIGLIGKYVELHDAYKSISEAFIHAGATNEVKVKIHWIHSEELKAADVAGLLKGLNGILVAPGFGERGIEGKIKAVQYARENNIPFFGICLGMQMAVIEFSRNVLKLADANSIEMDENTSNPVINLMEEQKEITNMGGTMRLGAWDCKLTENSKLKDIYNSEIISERHRHRYELNNKYVADLETAGMTVSGKNPVTDLVEVIEIPSHPWFIGVQYHPEYKSTVENPHPLFVAFVKAALK",
    "response#2": "MTKYIFVTGGVVSSVGKGIAVASIGTILKSRQITVSLMKLDQYLNVDPGTMSPYQHGEVFVTQDGAETDLDLGHYERFIDVNLTAESNVTSGQIYSAVIGKERRGEFLGGTIQVVPHVTAEIKKRFAKLAEKSQADVVIIEVGGTVGDIEGQHFYEAIRQMRNDVGRENVLYIHVTLLPYIATTQELKTKPTQHSVNELRRIGIQPDVIICRADLPIPEGIRDKISLFCDVDRDAVIPLPTIPSIYEVPLALEEQGIGHLIVDKLELEQRQPNLSKWEEFVNKFKNPAHQVTIGLVGKYIDLPDAYLSVTEALKAGGFAHHAKVSIRWVASDECETPEGAARNLADLDGMLIPGGFGVRGIQGKLGALKFARENGIPTLGLCLGLQCMVIEYARDIVGLAGASSSEFDPDTEFPVIATMAEQIAIIEGGDLGGTMRLGLYEAALKPGSIVASLYGSEHAAERHRHRYEVNNAYRQQIADAGLVFSGISPDRTLVEFVELPRDVHPFYVGTQAHPELRSRPNHAHPLFRGLISAALAYQKEKLADAYLSVVEALAHAGIRHGVEVDIRWVDAHELASPALTERALAGAHGVLVPGGFGLRGIEGKIAAARHARERHVPYLGICLGLQVAVIEFARHVLGLSEANSSEFDPDTPDAVVDLMADQRDVRDLGGTMRLGVYPCKVTEGTRTHAAYGEPVVYERHRHRWEVSNRYRQRLADAGMRFAGLSPDDRLVEIIELAEHPWFVASQFHPEFRSRPNRPQPLFREFLKVVEKKLS",
    "response#3": "MSTKYIFVTGGVVSSIGKGICAAAIGRILKSRGLTVAVQKLDPYLNVDPGTMSPYQHGEVFVTRDGCETDLDLGHYERFIDIELTHNSNITAGQVYTQLLGEERRGVHLGGTIQTVPHVTNVIKEFMLRAQHEGSPDVVLVEVGGTVGDIEGQPFLEAIRQMRMAVGRRNTFYVHVTLLPYIGATGELKTKPTQHSVKELRAIGIQPDAIICRSDHPIPHSIKEKIALFCDVAPDAVIPMETLSNIYQAPLVLEQEGVGEILVRELNLPTTTPDMTEWSDMVDRWKNPEGEVSIAIVGKYVNLRDAYLSVIEALRHGGFHHGAQVDITWVSSDELDQADAATVLSGIDGIVVPGGFGWRGIEGKLEAIHYARESGVPFLGLCLGLQCAVIEYARNVCGLEGANSSEFDPATPHPVIDLLPEQKNVTDLGGSMRLGAQPCHLVPGTRAAAAYGEQVVYERHRHRYEVNPAYHQILSEHGLVFSGLSPDGRLVEIIELPDHPFFMGGQFHPELRSRPTRPHPLFREFIGAAVDYRDRVEIAVVGKYVALADAYLSVVEALAHAGIRHGVEVDIRWVDAHELASPALTERALAGAHGVLVPGGFGLRGIEGKIAAARHARERHVPYLGICLGLQVAVIEFARHVLGLSEANSSEFDPDTPDAVVDLMADQRDVRDLGGTMRLGVYPCKVTEGTRTHAAYGEPVVYERHRHRWEVSNRYRQRLADAGMRFAGLSPDDRLVEIIELAEHPWFVASQFHPEFRSRPNRPQPLFREF"
  },
  {
    "reference": "MLVEARKKLIFALDVESGASAEEWVRRLRGKVGVFKVGKQLFTRCGPDVVRMIRDHGGEVFLDLKFHDIPNTVAKAAIEACRLGVRMFNVHALGGRAMMAGAAEAVREYFAAGEDVPPLLLGVTILTSSSEETLREVGVDRPVEEMVPRLACLAKGAGLDGVVASPREVGLIRRACGRDFAIVTPGVRPSFAETNDQQRIMTPGEAIAAGSDFLVVGRPIAASEDPVGAAQRIVEEMAAALEEG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should take into account the physiological conditions of the UMP from orotate: step 2/2 pathway. The designed protein sequence is ",
    "response#1": "SNLSVAVDLTKKSEILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVKLQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSYTEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIVNCGSDIIIVGRGVYGAGRNPVVEAKRR",
    "response#2": "SNLSVAVDLTKKSEILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVKLQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSYTEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIVNCGSDIIIVGRGVYGAGRNPVVEAKRC",
    "response#3": "NLSVAVDLTKKSEILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVKLQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSYTEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIVNCGSDIIIVGRGVYGAGRNPVVEAKRC"
  },
  {
    "reference": "MRVKEIMRNYQHLWKWGTMLLGMLMICSAAENLWVTVYYGVPVWKEATTTLFCASDAKAYDTEMHNVWATHACVPTDPNPQEVVLENVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCADVNSTTNNSSTGEMQEGEIKNCSFNTTTNIKDRIRKEYALFYKLDVVPIVDDGTNNTNATSTHYRLISCNTSVITQACPKISFEPIPIHYCAPAGFAILKCNDKKFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEGEVIIRSENLTNNAKTILVPLNKSVEIICERPNNNTRRSIPIGPGRVFFTSEIIGDIRQAYCTINGTTWSETLKQVAIKLGEKFPNKTIAFNRSSGGDPEIVMYSFNCGGEFFYCNSSKLFNNTWNGTNWTDTWYNSTNETIILPCRIKQVINMWQEVGKAMYAPPIRGKIKCKSNITGLLLTRDGGGGSNETTETFRPGGGNMRDNWRSELYKYKVVKIQPVGLAPTRAKRRVVQREKRAVTLGAVFLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQSNLLKAIEAQQHLLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTTVPWNNSWSNKSYDHIWGNMTWMEWEREIDNYTHLIYNLIEDSQNQQEKNELDLLELDKWASLWNWFDITKWLWYIKIFIMIVGGLIGLRLVFTVLSIVNKVRKGYSPLSFQTRLPAQRGPDRPEGTEEEGGEQGRGRSNRLVVGFLALIWDDLRSLCLFSY",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.2. The protein should be able to modulate positive regulation of establishment of T cell polarity, mitigation of host antiviral defense response in a way that leads to a desirable outcome.3. The protein should exhibit Basic and acidic residues compositional bias.4. The protein must exhibit the following characteristics: Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.5. Incorporating a Di-leucine internalization motif, YXXL motif; contains endocytosis signal motif is critical for the successful design of the protein.6. The designed protein must have at least one Retroviral envelope protein GP41-like, Human immunodeficiency virus 1 envelope glycoprotein Gp120 domain. The designed protein sequence is ",
    "response#1": "MWVTVYYGVPVWRDAETTLFCASDARAQDKEVHNVWATHACVPTDPDPQEIALENVTEEFNMWKNNMVEQMQTDIISLWDQSLKPCVKLTPLCVTLNCTEPIKPNSTESNSTXNNSMYEEMKNCSFNMTTEVRDRLKVVHSLFYKLDIVSTGRNDSKEYRLINCNXTSVTQACPKVTFEPIPIHYCAPAGFAILKCRDPXFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEXQVMIRSENITDNXKIIIVQLXEPVXITCIRPGXXTKRXXXXIGPGQTFYAXGXXXGDIRKAYCEVNKTEWNSALQXISXKLRNSYFXNKTIIFKNSSGGDLEITTHSFNCGGEFFYCYTTSLFNSTWNXTSINSTGXNETITLPCRIKQIXRMWQKVGQAMYAPPIXGVIRCXSNITGLLLTRDGAGGNNSNXETFRPIGGDMRDNWRSELYKYKVVKXEPIGXAPTXARRRVVGREKR",
    "response#2": "MWVTVYYGVPVWRDAETTLFCASDARAQDKEVHNVWATHACVPTDPDPQEIALENVTEEFNMWKNNMVEQMQTDIISLWDQSLKPCVKLTPLCVTLNCTEPIKPNSTESNSTXNNSMYEEMKNCSFNMTTEVRDRLKVVHSLFYKLDIVSTGRNDSKEYRLINCNXTSVTQACPKVTFEPIPIHYCAPAGFAILKCRDPXFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEXQVMIRSENITDNXKIIIVQLXEPVXITCIRPGXXTKRXXXXIGPGQTFYAXGXXXGDIRKAYCEVNKTEWNSALQXISXKLRNSYFXNKTIIFKNSSGGDLEITTHSFNCGGEFFYCYTTSLFNSTWNXTSINSTGXNETITLPCRIKQIXRMWQKVGQAMYAPPIXGVIRCXSNITGLLLTRDGAGGNNSNXETFRPIGGDMRDNWRSELYKYKVVKXEPIGXAPTXARRRVVGREKRAALGAMFLGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQNNLLKAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSKKNVDYIWDNMTWMQWENEIDNYTGIIYTLLEESQNQQEKNEQELLELDKWASLWSWFDISSWLWYIRIFIMIVGGLIGLRIIFAVISIVKRVRQGYSPLSLQ",
    "response#3": "MWVTVYYGVPVWRDAETTLFCASDARAQDKEVHNVWATHACVPTDPDPQEIALENVTEEFNMWKNNMVEQMQTDIISLWDQSLKPCVKLTPLCVTLNCTEPIKPNSTESNSTXNNSMYEEMKNCSFNMTTEVRDRLKVVHSLFYKLDIVSTGRNDSKEYRLINCNXTSVTQACPKVTFEPIPIHYCAPAGFAILKCRDPXFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEXQVMIRSENITDNXKIIIVQLXEPVXITCIRPGXXTKRXXXXIGPGQTFYAXGXXXGDIRKAYCEVNKTEWNSALQXISXKLRNSYFXNKTIIFKNSSGGDLEITTHSFNCGGEFFYCYTTSLFNSTWNXTSINSTGXNETITLPCRIKQIXRMWQKVGQAMYAPPIXGVIRCXSNITGLLLTRDGAGGNNSNXETFRPIGGDMRDNWRSELYKYKVVKXEPIGXAPTXARRRVVGREKRAALG"
  },
  {
    "reference": "MSTRAGRRVAISAGSLAVLLGALDTYVVVTIMRDIMHDVGIPVNQMQRITWIVTMYLLGYIAAMPLLSRASDRFGRKLLLQVSLAGFAIGSVMTALAGQFGDFHMLIAGRTIQGVASGALLPITLALGADLWAQRNRAGVLGGIGAAQELGSVLGPLYGIFIVWLFSDWRYVFWINIPLTAIAMLMIQVSLSAHDRGDELEKVDVVGGVLLAIALGLVVIGLYNPQPDSKQVLPSYGVPVLVGGIVATVAFAVWERCARTRLIDPAGVHIRPFLSALGASVAAGAALMVTLVNVELFGQGVLGMDQTQAAGLLVWFLIALPIGAVLGGWGATKAGDRTMTFVGLLITAGGYWLISHWPVDLLNYRRSIFGLFSVPTMYADLLVAGLGLGLVIGPLSSATLRVVPTAQHGIASAAVVVARMTGMLIGVAALTAWGLYRFNQILAGLSTAVPPDATLIERAAAVADQAKRAYTMMYSDIFMITAIVCVIGALLGLLISSRKEHASEPKVPE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that May be an efflux transporter and involved in maintaining correct cell wall permeability2. The protein must be able to transmembrane transporter activity. The designed protein sequence is ",
    "response#1": "QSFWLITGIIFIAFNLRPAITSVGPVISSIRAELHMSNGAAGFLTALPLLSFAVLSPLAPKLGQRLGNERTLWLGLVILLIGVLTRSTGYTAALFFGTALIGVGIAIGNVLLPSLIKHKYPEKPGIMISLYTTSMNIFAALASGVSVPLATQMNGGWKQAFLLWGGLALLALLIWIPQLRHRDTANQTMKLQSSSIWASKMAWYVTIFMGLQSFLFYSSIAWFPEILRSHGIDTATAGWMVSLMQFASLPSTFLTPVLADRVKQQRGIVAALASVYLIGLCGLLAGGSHTLLAIWMIIIGIGQGSSISLALTLIGLRSENAQQAAALSGMSQSFGYLLAAVGPIFVGYLFDQTHSWTMPIVLLIAALIVMGAAGQGAGRDRYR",
    "response#2": "QSFWLITGIIFIAFNLRPAITSVGPVISSIRAELHMSNGAAGFLTALPLLSFAVLSPLAPKLGQRLGNERTLWLGLVILLIGVLTRSTGYTAALFFGTALIGVGIAIGNVLLPSLIKHKYPEKPGIMISLYTTSMNIFAALASGVSVPLATQMNGGWKQAFLLWGGLALLALLIWIPQLRHRDTANQTMKLQSSSIWASKMAWYVTIFMGLQSFLFYSSIAWFPEILRSHGIDTATAGWMVSLMQFASLPSTFLTPVLADRVKQQRGIVAALASVYLIGLCGLLAGGSHTLLAIWMIIIGIGQGSSISLALTLIGLRSENAQQAAALSGMSQSFGYLLAAVGPIFVGYLFDQTHSWTMPIVLLIAALIVMGAAGQGAGRDRYR",
    "response#3": "QSFWLITGIIFIAFNLRPAITSVGPVISSIRAELHMSNGAAGFLTALPLLSFAVLSPLAPKLGQRLGNERTLWLGLVILLIGVLTRSTGYTAALFFGTALIGVGIAIGNVLLPSLIKHKYPEKPGIMISLYTTSMNIFAALASGVSVPLATQMNGGWKQAFLLWGGLALLALLIWIPQLRHRDTANQTMKLQSSSIWASKMAWYVTIFMGLQSFLFYSSIAWFPEILRSHGIDTATAGWMVSLMQFASLPSTFLTPVLADRVKQQRGIVAALASVYLIGLCGLLAGGSHTLLAIWMIIIGIGQGSSISLALTLIGLRSENAQQAAALSGMSQSFGYLLAAVGPIFVGYLFDQTHSWTMPIVLLIAALIVMGAAGQGAGRDRYRR"
  },
  {
    "reference": "MSDIEGIERAISDIAAGRPVIVVDDEDRENEGDLIFAAEKATPELLAFMVRYTSGYICVPLTESDCDRLDLPPMFHTNQDVRGTAYTVTVDAKEGVSTGISAADRAHTIRLLANRKSEAGDFTRPGHVVPLRAKEGGVLRRPGHTEAAVDLAVLAGLRPAGVLCEIVSQKDEGDMARREELEVFAAEHDLAIISIADLIDYRRRKEVQVVRVAEARIPTAHGAFRAVGYDSKLDGIEHIALVYGDIEDGENVLVRVHSECLTGDVFGSLRCDCGPQLDAALAAVAAEGRGVVLYMRGHEGRGIGLMHKLQAYQLQDSGADTVDANLALGVPADARDYGQGAQILVDLGIKSMRLLTNNPAKRVGLDGYGLTIVDRVPLPIRPNAENLRYLRTKRDRMGHELGDLDIFEGTTQT",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Ideal protein should have a binding affinity for key enzymes in the Cofactor biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway to enhance the overall efficiency of the pathway.2. A protein with GTP cyclohydrolase II activity, GTP binding, magnesium ion binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, zinc ion binding is needed to enhance riboflavin biosynthetic process in a specific context.3. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..4. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor; for GTP cyclohydrolase activity, Nucleophile; for GTP cyclohydrolase activity to enable effective catalysis of GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.5. The designed protein have GTP cyclohydrolase II domains with specific sequence motifs that are crucial for its activity.6. The protein must bind to Zn(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MALKDILKNVEIVGVNGSLDVAXAXVMDVXNDSRAVNPGCLFFCKGAKFKPEFLENVLESGVSIYVSETPYDAEVPQIVTSDIKKAMAIISANFYGNPSEKMKVVGFVATTGTKGKTTTANIIHSILSKSYKTAMISSVXIKIGDVEIPQNRTTPESLELHSIMFKMREAGVSYCVMEVSSHALXMDRVAGLXYXIGIFTNLXEDHLDEHGSMXDYKNAKGLLFSXLGNAFVCNADDXNWKEILEGHTCKIETYSIDXKADFRAANIXVDSRGXSFXVEYLGKKXDTXLPMIGKFNVYNALSAIAVSXLEGVELEDIKESLXGVNVPDRMXPIFINEGVTMVNDXYAXNXEAXVXTLSSFXKXKXLVXGXDRMVSTVLPTRNGRFDVHLYRSAVDTKEHIALVKGTIDAGAPVLVRVHSECLTGDIFGSQRCDCNEQLHTSMEMIEREGNGIVLYMRQEGRGIGLLNKLRAYKLQDEGKDTVEANEALGFHADVRDYSLAAQILLDLGVRRVRLLTNNPKKIVGLKGFGIEVTERIFVXGVXKXDXLKXIXEXLXEAAXGXKYXTCXCGXE",
    "response#2": "RMVSTVLPTRNGRFDVHLYRSAVDTKEHIALVKGTIDAGAPVLVRVHSECLTGDIFGSQRCDCNEQLHTSMEMIEREGNGIVLYMRQEGRGIGLLNKLRAYKLQDEGKDTVEANEALGFHADVRDYSLAAQILLDLGVRRVRLLTNNPKKIVGLKGFGIEVTERIPHPDVPNRVAEATLPTKYGGEFKIIVYENEVDDMKHIALVKGDIQPEDEVLVRVHSECVTGDLFGSLRCDCGDQLHKAMEMVGQEGKGVIVYMHQEGRGIGLANKIRAYDLQEQGRDTVEANIELGFKEDLRDYGIGAQILVDLGVRKMRMITNNPKKIVGVEGYGLTVVERVPHPDVPGVREAEAKLPSAFGDFRIVAYTNELDGKEHVAIVKGEIDSSKPVLVRVHSECLTGDIFGSHRCDCGPQLHAALRMIEAEGRGIVLYMRQEGRGIGLINKLKAYRLQEQGYDTVEANKKLGFPADLRDYGIGAQILLDLGVKQMRLMTNNPRKIRGLSGYGMQVAERVPHPDVPGVSAEEVRVADARIPTEHGTFRAVGYQSSFDEAEHVALVMGDIAADGGEDVLVRVHSECLTGDVFGSLRCDCGPQLDAAMEMVGREGRGVILYMRGHEGRGIGLMHKLQAYQLQDNGADTVDANLELGLPADARDYGLGAQILVDLGVRSMRLLTNNPAKRVGLDGYGLEIVERPHPDVPGVSAEEREVVAQLPSEFGDFRIAAYTNLLDGKEHVALIKGEISSDEPILVRVHSECLTGDVFGSLRCDCGPQLHAALEQIEQAGSGVLLYLRQEGRGIGLINKLRAYNLQEQGFDTVEANEKLGFAADLREYGIGAQMLRDLGVRGMRLMTNNPRKIRGLEGYGLKVIERV",
    "response#3": "KVAEAQLPSRYGGDWKAIAFENDIDKLDHLALVKGDINTRVPVLVRVHSECLTGDVLGSLRCDCGEQLHQAMTMIERAGKGVILYMRQEGRGIGLINKLKAYELQDQGRDTVEANLELGFKADLRDYGIGAQILLSLGIHKMRLMTNNPKKLIGLEGYGIDIVERDPSDRIVPENPRVAEATLPTKYGGEFKIIVYENEVDDMKHIALVKGDIQPEDEVLVRVHSECVTGDLFGSLRCDCGDQLHKAMEMVGQEGKGVIVYMHQEGRGIGLANKIRAYDLQEQGRDTVEANIELGFKEDLRDYGIGAQILVDLGVRKMRMITNNPKKIVGVEGYGLTVVERVREVEVKMPTEFGVFKAVAYTNDVDNKEHVALVKGEIDSSQPTLVRVHSECLTGDVFHSHRCDCGPQLEAALRQIDSEGSGVLLYMRQEGRGIGLINKLKAYKLQEEGLDTVEANQKLGFAPDLRDYGIGAQILKDLGVSQIKLLTNNPKKIRGLEGYGLEVVERVPYEEV"
  },
  {
    "reference": "MEGLQRCFVLHYRPYSETSLILDVFSEDYGRLTILSKGARRKRSNLKGTLQPFTPLFMKWTGRGSMKTLTHAEPISISLPMRGYILYSAFYLNEVLVRVLENDTPYPVLFMDYLNALRELAQADNPEPALRRFELALLHHLGYGIDFLHCAGSGLPIDDLMTYHYREQKGFIASMMTGHFTFTGNQLLAIAARRFETPDQLKAAKRFTRIALKPYLGGKPLKSRELFIPRTRNIGK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design must be focused on enhancing the efficiency of DNA recombination, DNA repair. The designed protein sequence is ",
    "response#1": "MVLTTGVKGLDELLGGGVARRVILQVYGPFATGKTTFAMQVGLLNEGKVAYVDTEGGFSPERLAQMARSRGLDPEKALSKFIIFEPMDLNEQRRVISKLKTIVDEKFSLVVVDSLTAHYRAEGSKNYGELAKQLQVLQWLARRKNVAVIVINQVYHDSNSNSLRPIAEHTLGYRTKDILRFEKLRVGVRLAVLERHRFRPEGGIVYFKITDKGLEA",
    "response#2": "MVLTTGVKGLDELLGGGVARRVILQVYGPFATGKTTFAMQVGLLNEGKVAYVDTEGGFSPERLAQMARSRGLDPEKALSKFIIFEPMDLNEQRRVISKLKTIVDEKFSLVVVDSLTAHYRAEGSKNYGELAKQLQVLQWLARRKNVAVIVINQVYHDSNSNSLRPIAEHTLGYRTKDILRFEKLRVGVRLAVLERHRFRPEGGIVYFKITDKGLEV",
    "response#3": "MVLTTGVKGLDELLGGGVARRVILQVYGPFATGKTTFAMQVGLLNEGKVAYVDTEGGFSPERLAQMARSRGLDPEKALSKFIIFEPMDLNEQRRVISKLKTIVDEKFSLVVVDSLTAHYRAEGSKNYGELAKQLQVLQWLARRKNVAVIVINQVYHDSNSNSLRPIAEHTLGYRTKDILRFEKLRVGVRLAVLERHRFRPEGGIVYFKITDKGLEV"
  },
  {
    "reference": "MIEVLHQAGRFPLFSSEGCRALEAASLARSPAQALMARAGLAVARLALAVAPHARRVWIACGPGNNGGDGLVAARHLASLGLEVHVSHLCGSKPLPADAQWAWRTAMDAGLRIGSTLQPPEGCELVIDALLGLGLQRAPEAALEQAILAINACDVPVLSIDLPSGLQADAGRPCPDALGRAGAVVRAQHTLCLLGLKPGLFTGQGRDHAGRIWFDDLGVSAADGDCGRLVALGERQRWQALARRTHASHKGSYGQVLVIGGAAHMEGAALLAAGSALAAGAGKVHLKPLSGAVHAGGRPELMRWPEGPLDSQTPWQDLTLVVGCGAGHGLDAGPATGSGSPLPEVLRQAPRLVLDADGLNSVARSGATRELLHARRSAGRQTILTPHPLEAARLLAWPVAQVQADRLVAARALSATLDCSVVLKGSGSVIASPGEPLHVNGSGNAALAGAGTGDVLAGWLGGLWAQRPDLPPHEIATMGVAWHGEAAQGAQAPLRAADLIERMHALQACLP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein have enhanced (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "WHEIGPPLLPPLDAAGHKFSRGLVHALAGKMPGAIALAATAAARAGAGYVRVSTSRPIDGLPTAVVQTDKAEVNDARIGCLLVGPGMGDIPQLLTLALTSHAPKVIDADGINHVGEPDRLRGQDAVITPHADEFARLFGSTPGSKAEQALEAARRSGAVVVYKGPDTLVAAPDGRVGFAPPAPAWLASAGTGDVLAGIIATMRARGLPGFEAACAGVWLHGRAAEIAGAHMIADDLADAMPAALL",
    "response#2": "LPPLDAAGHKFSRGLVHALAGKMPGAIALAATAAARAGAGYVRVSTSRPIDGLPTAVVQTDKAEVNDARIGCLLVGPGMGDIPQLLTLALTSHAPKVIDADGINHVGEPDRLRGQDAVITPHADEFARLFGSTPGSKAEQALEAARRSGAVVVYKGPDTLVAAPDGRVGFAPPAPAWLASAGTGDVLAGIIATMRARGLPGFEAACAGVWLHGRAAEIAGAHMIADDLADAML",
    "response#3": "LPPLDAAGHKFSRGLVHALAGKMPGAIALAATAAARAGAGYVRVSTSRPIDGLPTAVVQTDKAEVNDARIGCLLVGPGMGDIPQLLTLALTSHAPKVIDADGINHVGEPDRLRGQDAVITPHADEFARLFGSTPGSKAEQALEAARRSGAVVVYKGPDTLVAAPDGRVGFAPPAPAWLASAGTGDVLAGIIATMRARGLPGFEAACAGVWLHGRAAEIAGAHMIADDLADAMA"
  },
  {
    "reference": "MPKNRGVLDAITRSVIDGSDQESSSSFNSDKEYAAVTKGLSSSRVQKKSSLRQMKSKVKELQSLVNHYRENEAALVSSAKLLSGEIIGYEIKMASLHGKMKSILDENNALKETHKSSAEKRIELVRLPSSKEERNYDEYTLLVNLKKEICAKLQDYKNVQNTVNTKLDEIHTFHEKYYEGLELSLDSKVFDAESSKELAKVRRELNNVRKNSEIKVNNLKMQLLQATKSLEHLKKQAKAKDDYLKCIPELVDKANLTMLSYKKSIANQRETIEALQAELSQQSETKGQIETETQNQVQIPTNVTLVDPFEENNPEDLFAIQEQELQDLRLHKKMADERSRTTHLHLERKNNTIKLLQSYVQSLIQRLPPAQRKHHLGIFQKLGSEKSCPLAPAVASTYAPLLLLSQHSNHQEIDNTPQRLLLAAPDGQSYSEKSTTLNLDYSSRKSYLSRLQPPHIANLKSLTLKTLPRVPTDSPQLPSKDKSQETAKKDDRPKLVANEPVTLDTSTPPVAQSLADSKHCSGLHK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be expressed specifically in the nucleus2. The protein should exhibit Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "EDLSRDILKSDTANVIIPELEIEVTHGSLGGKFTTVEGLITIIKEELEKNPFFRGDSSDPANRARYQEITSQLDSFIAGKKPFTIELDDPISNSYIQNLFAPDDDPQLKIVEYERTYEQNDELGC",
    "response#2": "DWLMEVCEVYKLYRETFYLAQDFFDRFMATQQNVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACTEDEILSMELIIMR",
    "response#3": "LTVVSWLNIYMQVAYLNELYEVLLPQYPQQIFVQIAELLDLCVLDIGCLEYTYGVLAASALYHFSSSELMQKVSGYEWCEIEECVKWMVPFAMAIREVGSSKLKHFRGIAPEDLHNIQTHINC"
  },
  {
    "reference": "MSLSAAEVNKIAWLARLAIDDDKVEAYARDLSQILGFVEQLGSVDTSRVAPMAHPLDEAQRLRPDDVTETDQRALFQAHAPLVEAGLYLVPKVIE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp- tRNA(Asn) or phospho-Glu-tRNA(Gln).2. A protein with asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity is needed to enhance translation in a specific context. The designed protein sequence is ",
    "response#1": "VMGMEVHVELSTATKMFCGCANAFGAEPNTQVCPVCLGLPGSLPVLNQQAVESAIRIGLALNCEIVPWCRFARKNYFYPDQPKNYQISQYDEPIAINGYLDVPLDDGSTWRVEIERAHMEEDTGKLTHLGSDTGRIAGATNSLADYNRAGVPLIEIVTRPIEGAGVKAPEIARAYVTALRDLLRALGVSDVRMDQGSMRCDANLSLKPIGQAEFGTRTETKNVNSLKSVEVAVRYEMRRQAAVLTSGGQVHQETRHFHEDGYTSPGRSKETAEDYRYFPEPDLEPVAPDAEFVEELEARFPPAR",
    "response#2": "VISDYLQRIDSSRDDNIFTVVFHDEAMARARELDSKLQRGEAPGVLFGMPIAIKDNIAMKGAPLSCASKILAGYESVYDATVIKRMQAEDAIFVGRTNMDEFAMGSSNENSAIGPVPNPYDKTRVPGGSSGGSAAAVANDLAMVALGSDTGGSVRQPAGFCNIIGLKPTYGRISRYGLVAFASSFDQIGLLAANCDDAALVLGVIAGKDEHDATSSHHDVPEYDTAMDHVSVDGLRIGVPRAFFPESLNADVAGVVKAGLKKLEEAGAELVEIDLPESDYAIAAYYILVTAEASSNLARFDGARYGYRSPDSPDLSSMYVNSRTEGFGAEVKRRIMLGTYVLSAGYYDTYYKKAQQVRRVFQDKYREAFEKVDVIFGPTSPFPPFGIGDKMDNPLEMYLADVFTVPASIVGMPAISVPVGFDSLGLPVGAHLICNFFEEGKLYRMGEINGMV",
    "response#3": "VMGMEVHVELSTATKMFCGCANAFGAEPNTQVCPVCLGLPGSLPVLNQQAVESAIRIGLALNCEIVPWCRFARKNYFYPDQPKNYQISQYDEPIAINGYLDVPLDDGSTWRVEIERAHMEEDTGKLTHLGSDTGRIAGATNSLADYNRAGVPLIEIVTRPIEGAGVKAPEIARAYVTALRDLLRALGVSDVRMDQGSMRCDANLSLKPIGQAEFGTRTETKNVNSLKSVEVAVRYEMRRQAAVLTSGGQVHQETRHFHEDGYTSPGRSKETAEDYRYFPEPDLEPVAPDAEFVERYRQMGIPPT"
  },
  {
    "reference": "MAEDSGKKKRRKNFEAMFKGILQSGLDNFVINHMLKNNVAGQTSIQTLVPNTDQKSTSVKKDNHKKKTVKMLEYLGKDVLHGVFNYLAKHDVLTLKEEEKKKYYDTKIEDKALILVDSLRKNRVAHQMFTQTLLNMDQKITSVKPLLQIEAGPPESAESTNILKLCPREEFLRLCKKNHDEIYPIKKREDRRRLALIICNTKFDHLPARNGAHYDIVGMKRLLQGLGYTVVDEKNLTARDMESVLRAFAARPEHKSSDSTFLVLMSHGILEGICGTAHKKKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGEKHGELWVRDSPASLALISSQSSENLEADSVCKIHEEKDFIAFCSSTPHNVSWRDRTRGSIFITELITCFQKYSCCCHLMEIFRKVQKSFEVPQAKAQMPTIERATLTRDFYLFPGN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Initiates pyroptosis, a programmed lytic cell death pathway through cleavage of Gasdermin-D (GSDMD): cleavage releases the N-terminal gasdermin moiety (Gasdermin- D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis; During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation.2. The designed protein should contain one or more CARD domains that are essential for its function.3. A protein that can perform cysteine-type endopeptidase activity involved in apoptotic signaling pathway, cysteine-type endopeptidase activity, cysteine-type peptidase activity in cytosol, cytoplasm, NLRP1 inflammasome complex to support substantia nigra development, regulation of inflammatory response, cellular response to mechanical stimulus, apoptotic process.4. The designed protein is the optimization of the active site to achieve high catalytic efficiency for Strict requirement for Asp at the P1 position. It has a   preferred cleavage sequence of Tyr-Val-Ala-Asp-|- but also cleaves at   Asp-Glu-Val-Asp-|-.. The designed protein sequence is ",
    "response#1": "MFAKATRSFLREVDAEGDLIAVSNLNDSDKSQLLSLVTKKKRFWCWQRPKYQFLSVTLGDVLTEAQCLSPVVVESDFVKYEGKFENHVSGTIETALGKVKLNFGDKGLRESQSSFGTLRKQEVDLQQLIRDSVERTINLKNPVLQQMLESKNEVLCILTQKIVTTQKCVISEHIQTEEKCGGMVGIKTKTVQVSVTKDENIIKDASVALEIPAPTTIAYSVIELYVKLDGQFEFCLLRGKHGGFEAVYKTHVEKDFIAFCSSTPHNVSWRDIKKGSLFITRLITCFQKYAWCCHLEEVFRKVQQSFEKPNVKAQMPTVERLSMTRYFYLFGNSGPIYPIKERKDRTRLALIICNTEFDHMPPRNGAALDILGMKQLLEGLGYTVEVEEKLTARDMESVLWKFAAREEHKSSDSTFLVFMSHGILDGICGTMHSEEEPDVLPYDTIFRTFNNRNCLSLKDKPKVIIVQACRGANRGELWVSDSPPALADSFSQSSENLEEDAVYKTHVEKDFIAFCSSTPHNVSWRDIKKGSLFITRLITCFQKYAWCCHLEEVFRKVQQSFEKPNVKAQMPTVERLSMTRYFYLKNDSCNGDSGSPLDSIIGGNEVTPHSRPYMVLLSLDRKTICAGALIAKDWVLTAAHCNLNKRSQVILGAHSITREEPTKQIMLVKKEFPYPCYDPATREGDLKLLQLMEKAKINKYVTILHLPKKGDDVKPGTMCQVAGWGRTHNSASWSDTLREVNITIIDRKVCNDRNHYNFNPVIGMNMVCAGSLRGGRDSCNGDSGSPLLCEGVFRGVTSFGLENKCGDPRGPGVYILLSKKHLNWIIMTI",
    "response#2": "MFAKATRSFLREVDAEGDLIAVSNLNDSDKSQLLSLVTKKKRFWCWQRPKYQFLSVTLGDVLTEAQCLSPVVVESDFVKYEGKFENHVSGTIETALGKVKLNFGDKGLRESQSSFGTLRKQEVDLQQLIRDSVERTINLKNPVLQQMLESKNEVLCILTQKIVTTQKCVISEHIQTEEKCGGMVGIKTKTVQVSVTKDENIIKDASVALEIPAPTTIAYSVIELYVKLDGQFEFCLLRGKHGGFEPLSVLKQGTRLLEKNFFPFVELPEQHRTALNTVLQAVLSDEELLAVLEQVCDDLVHSLSPPLAMLGELKPPHRQDLTAFLRLVGYRVQGGCPCLEDGVGSQKLFSTAYFLVSALAEMPDNAAALLGTCCKLQIIPALCHLLHAMSHDGVCDLEDPALAPLKDTERFGVAQRLFASADINLERVQSSVAIYKVHVEKDFIAFCSSTPHHVSWRDVNKGSLFITQLITCFQKYSWCFHLEEVFRKVQQSFEKPNVRAQMPTIERLSMTRYFYLFAIYKVHVEKDFIAFCSSTPHHVSWRDVNKGSLFITQLITCFQKYSWCFHLEEVFRKVQQSFEKPNVRAQMPTIERLSMTRYFYLFSDSCNGDSGSPLDVYQMKSKPRGYCLIFNNNNFSKAREDIPKLSNMRDRKGTNYDEEALSKTFKELHFEIVSFSDCTASQIHEVLVSYQSKDHKGKDCFICCILSHGDKGIVYGTDGKEASIYELTSYFTGSKCPSLAGKPKIFFIQACQGNNFQKAVPVPDETGLEQEHVLEEDSSSYKNYIPDEADFLLGMATVKNCVSYRDPTRGTWYIQSLCQSLRERCPRGEDILSILTGVNYDVSNKDNPRNMGKQMPQPIFTLRKKLFF",
    "response#3": "MFAKATRSFLREVDAEGDLIAVSNLNDSDKSQLLSLVTKKKRFWCWQRPKYQFLSVTLGDVLTEAQCLSPVVVESDFVKYEGKFENHVSGTIETALGKVKLNFGDKGLRESQSSFGTLRKQEVDLQQLIRDSVERTINLKNPVLQQMLESKNEVLCILTQKIVTTQKCVISEHIQTEEKCGGMVGIKTKTVQVSVTKDENIIKDASVALEIPAPTTIAYSVIELYVKLDGQFEFCLLRGKHGGFEPLSVLKQGTRLLEKNFFPFVELPEQHRTALNTVLQAVLSDEELLAVLEQVCDDLVHSLSPPLAMLGELKPPHRQDLTAFLRLVGYRVQGGCPCLEDGVGSQKLFSTAYFLVSALAEMPDNAAALLGTCCKLQIIPALCHLLHAMSHDGVCDLEDPALAPLKDTERFGVAQRLFASADINLERVQSSVAVYKTHVEKDFIAFCSSTPHNVSWRDIKKGSLFITRLITCFQKYAWCCHLEEVFRKVQQSFEKPNVKAQMPTVERLSMTRYFYLFAIYKVHVEKDFIAFCSSTPHHVSWRDVNKGSLFITQLITCFQKYSWCFHLEEVFRKVQQSFEKPNVRAQMPTIERLSMTRYFYLFDSESGLFLFQASNSLFHCRDGGKNGFMVSPMKPLEIKTQCSGPRMDPKICPADPAFFSFINNSDLWVANIETGEERRLTFCHQGLSNVLDDPKSAGVATFVIQEEFDRFTGYWWCPTASWEGSEGLKTLRILYEEVDESEVEVIHVPSPALEERKTDSYRYPRTGSKNPKIALKLAEFQTDSQGKIVSTQEKELVQPFSSLFPKVEYIARAGWTRDGKYAWAMFLDRPQQWLQLVLLPPALFIPSTENEEQRLASARAVPRNVQPYVVYEEVTNVWINVHDIFYPFPQSEGEDELCFLRANECKTGFCHLYKVTAVLKSQGYDWSEPFSPGEDEFKCPIKEEIALTSGEWEVLARHGSKIWVNEETKLVYFQGTKDTPLEHHLYVVSYEAAGEIVRLTTPGFSHSCSMSQNFDMFVSHYSSVSTP"
  },
  {
    "reference": "SPWSSCSVTCGSGVITRIRLCNSPLPQMGGKECEGEARENVPCEKSPCPINGNWGPWSPWDACPVTCGGGVQKRGRLCNNPEPQYGGKDCIGEVSDTQICNKQNCPIDGCLSNPCFAGTTCTSFPDGSWKCGACPAGYHGNGVKCKDIDECKEVPDACFVLGGVHRCENTEPGYNCLPCPPRFTGTQPFGRGVDHAIANKQVCKPRNPCTDGTHDCNKHARCIYLGHFSDPMYRCECKPGYAGNGNICGEDTDLDGWPNENLVCVANATYHCNKDNCPTFPISGQENMRKEGMGDDNCPFIYNPQQYDYDRDDVGDRCDNCPYNHNPDQIDTDNNGEGDACAVDIDGDGILNERDNCQYVYNVDQKDTDLDGVGDQCDNCPMEHNPEQTDSDSDRIGDTCDSNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRLVPNPDQLDSDGDGRGDACKDDFDKDNVLDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGFDEFNAVDFSGTFFINTDRDDDYAGFVFGYQSSSRFYVVMWKQVTQTYWDIQPTKAQGYSGLSIKVVNSTTGPGTHLRNALWHTGNTAGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGYIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRGKCPHFHTIVFFLSNFYDGFLIHFQSLQFYQFKGFVKTFKSYLVTLLCDVDYIENN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must have at least one EGF-like domain.2. The protein design should emphasize Basic and acidic residues compositional bias.3. A protein that contributes to positive regulation of macrophage activation by functioning in platelet alpha granule with low-density lipoprotein particle binding. The designed protein sequence is ",
    "response#1": "GNGNEGSKVGSCPCDHTVSSHSPPNENIMRHLRKYLKAYQRCFSYVRFQLPLKNVCGGSTDGWVQELMHCFDSGECGHAQPRVVA",
    "response#2": "KASCKLSVKKATVLYQGQRVKIQDQFKNGMMHGDKVHFYCKNKEKKCSYTEEAQCIDGTIEIPKCFKEHSSLAFWKTDASDVTPS",
    "response#3": "DPNTCSFWESFTTTTKESHSRPFSLLPSEPCERPWEGPHTCPQPTVVYRTVYRQVVKTDHRQRLQCCHGFYESRGFCVS"
  },
  {
    "reference": "MRKFADDTLLVATHNRGKLEEIVTLLAPYGVRVIGAAERGLPEPSETETTFVGNARIKAHAAARASGLMSLSDDSGIVIDALGGAPGVYTADWAETGNGRDFVLAMTRAHDMLIASKEPQPWTARFCCTLVLAWPDGHDEVFEGTVEGWFVWPMRGDQGHGYDPIFQPLGHDITFGEMDRWEKNRISHRADAFQKMVKACFG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Mg(2+) is required for the proper folding and stability of the protein.2. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.3. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for dITP + H2O = dIMP + diphosphate + H(+).4. The protein should be able to bind substrate, Mg(2+) ligand in a variety of conditions5. The designed protein must possess ITP diphosphatase activity, XTP diphosphatase activity, ribonucleoside triphosphate phosphatase activity, metal ion binding, dITP diphosphatase activity to support purine nucleoside triphosphate catabolic process, nucleotide metabolic process under certain conditions. The designed protein sequence is ",
    "response#1": "MKVLITAGPTREPLDPVRFIGNRSSGKMGYALAQALRERGHAVTLVSGPVRLEPPRGVELVAVETSRDLHAAMLAQLPECDVFISAAAVSDFRPRTVAEQKIKKDGETLTLELVENPDILAEIGERKGPQFVVGFAAETDDVAANAEAKLRKKNLDFIAANLVSEPGAFGDDTNRVTLIDAEGRVEELPLMSKREVARRLLDRVAALRREPKPS",
    "response#2": "MKVLFASSNMNKLREIRNMLGDSIELLSLSDIGCTEEIPETSETLEGNALLKAEYVYSQYGMDCFADDTGLEVEALNNEPGVFSARYSGSRDSLQNMDKLLTKLQGNTNRSAQFKTVIALNLNGKQHLFTGICEGTIIQEQKGTEGFGYDPIFQPAGYNQTFAEMDLATKNKISHRGKALQKLIAFLNQ",
    "response#3": "MKIVLASNNQGKIKELRQLLAEADLEVESQADLNVPEAPEPHRTFVENALAKARHASAATGLPAIADDSGLCVDALQGMPGVLSARYAGEHADDAANNAKLLHELSGVPEAQRTARFHCTLVYMRHADDPTPIIAEGRWHGEIALQPKGQGGFGYDPLFWVPQHDCSSAELSSSAKNALSHRGQALRKLLAALAARK"
  },
  {
    "reference": "MSPQTETKAGVGFQAGVKDYKLTYYTPEYETKDTDILAAFRVSPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYHIEPVAGEDNQWICYVAYPLDLFEEGSVTNMFTSIVGNVFGFKALRALRLEDLRIPPTYSKTFQGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRACYECLRGGLDFTKDDENVNSQPFMRWRDRFVFCAEAIYKSQAETGEIKGHYLNATAGTCEEMIKRAVFARELGVPIVMHDYLTGGFTANTTLAHYCRDNGLLLHIHRAMHAVIDRQKNHGMHXXXXXXXXXXXGGDHIHSGTVVGKLEGEREMTLGFVDLLRDDFIEKDRARGIFFTQDWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPWGNAPGAAANRVALEACVQARNEGRDLAREGNEIIRAACKWSPELAAACEVWKAIKFEFEPVDTID",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Both reactions occur simultaneously and in competition at the same active site..2. The designed protein should have magnesium ion binding, monooxygenase activity, ribulose-bisphosphate carboxylase activity within chloroplast to regulate photorespiration, reductive pentose-phosphate cycle.3. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.4. The protein with the reaction D-ribulose 1,5-bisphosphate + O2 = (2R)-3-phosphoglycerate +   2-phosphoglycolate + 2 H(+), the Proton acceptor must be optimized to ensure efficient catalysis.5. The designed protein have Ribulose bisphosphate carboxylase large subunit ferrodoxin-like N-terminal, Ribulose bisphosphate carboxylase large subunit C-terminal domains with specific sequence motifs that are crucial for its activity.6. The substrate, Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MNISALWKVLGRPEVDGGLVVGTIIKPKLGLRPKPFAEACHAFWLGGDFIKNDEPQGNQPFAPLRDTIALVADAMRRAQDETGEAKLFSANITADDPFEIIARGEYVLETFGENASHVALLVDGYVAGAAAITTARRRFPDNFLHYHRAGHGAVTSPQSKRGYTAFVHCKMARLQGASGIHTGTMGFGKMEGESSDRAIAYMLTQDEAQGPFYRQSWGGMKACTPIISGGMNALRMPGFFENLGNANVILTAGGGAFGHIDGPVAGARSLRQAWQAWRDGVPVLDYAREHKELARAFESGADAFITGNYPVLLERVREALG",
    "response#2": "YYTPEYQTKDTDILAAFRVTPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYDIEPVPGEETQFIAYVAYPLDLFEEGSVTNLFTSIVGNVFGFKALRALRLEDLRIPPAYNISALWKVLGRPEVDGGLVVGTIIKPKLGLRPKPFAEACHAFWLGGDFIKNDEPQGNQPFAPLRDTIALVADAMRRAQDETGEAKLFSANITADDPFEIIARGEYVLETFGENASHVALLVDGYVAGAAAITTARRRFPDNFLHYHRAGHGAVTSPQSKRGYTAFVHCKMARLQGASGIHTGTMGFGKMEGESSDRAIAYMLTQDEAQGPFYRQSWGGMKACTPIISGGMNALRMPGFFENLGNANVILTAGGGAFGHIDGPVAGARSLRQAWQAWRDGVPVLDYAREHKELARAFESYSNCPPELAAFDDKEEDLIAGGEHVLCAYIMKPKAGYGYVATAAHFAAESSTGTNVEVCTTDDFTRGVDALVYEVDEARELTKIAYPVALFHRNITDGKAMIASFLTLTMGNNQGMGDVEYAKMHDFYVPEAY",
    "response#3": "MGYKEEDLIAGGEHVLCAYIMKPKAGYGYVATAAHFAAESSTGTNVEVCTTDDFTRGVDALVYEVDEARELTKIAYPVALFHRNITDGKAMIASFLTLTMGNNQGMGDVEYAKMHDFYVPEANHDYFAQEKLKKFETLSYLPPLSEESLLKEVQYLLNNGWVPCLEFEPTHGFVYREHGNTPGYYDGRYWTMWKLPMFGCTDPSQVVAELEEAKKAYPEAFIRIIGFDNVRQVQCVSFIAYKPDRSPAGLDFLKDDERNISALWKVLGRPEVDGGLVVGTIIKPKLGLRPKPFAEACHAFWLGGDFIKNDEPQGNQPFAPLRDTIALVADAMRRAQDETGEAKLFSANITADDPFEIIARGEYVLETFGENASHVALLVDGYVAGAAAITTARRRFPDNFLHYHRAGHGAVTSPQSKRGYTAFVHCKMARLQGASGIHTGTMGFGKMEGESSDRAIAYMLTQDEAQGPFYRQSWGGMKACTPIISGGMNALRMPGFFENLGNANVILTAGGGAFGHIDGPVAGARSLRQAWQAWRDGVPVLDYAREHKELARAFESWQKADHCLEPKEEDLIAGGEHVLCAYIMKPKAGYGYVATAAHFAAESSTGTNVEVCTTDDFTRGVDALVYEVDEARELTKIAYPVALFHRNITDGKAMIASFLTLTMGNNQGMGDVEYAKMHDFYVPEANHDYFAQEKLY"
  },
  {
    "reference": "MTNPAYFPQLSQLDVSGEMESTYEDIRLTLRVPWVAFGCRVLATFPGYLPLAWRRSAEALITRYAEQAADELRERSLLNIGPLPNLKERLYAAGFDDGEIEKVRRVLYAFNYGNPKYLLLITALSESMQMRPVGGAEVSSELRASIPKGHPKGMDPLLPLVDATKASTEVQGLLKRVADLHYHHGPASDFQALANWPKVLQIVTDEVLAPVARTEQYDAKSRELVTRARELVRGLPGSAGVQRSELMSMLTPNELAGLTGVLFMYQRFIADITISIIHITECLDGAEAASKSPFPI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must have high specificity towards DL-2 haloacid dehalogenase activity.2. The protein must exhibit the following characteristics: Acts on 2-haloalkanoic acids whose carbon chain lengths are five or less.; Dehalogenates both (S)- and (R)-2-haloalkanoic acids to the corresponding (R)- and (S)-hydroxyalkanoic acids, respectively, with inversion of configuration at C-2.3. The protein should have a low Km value for an (R)-2-haloacid + H2O = a (2S)-2-hydroxycarboxylate + a   halide anion + H(+). The designed protein sequence is ",
    "response#1": "EATGRR",
    "response#2": "LVSGFL",
    "response#3": "MNNNNSNGNTNGLIGK"
  },
  {
    "reference": "MVFMLYTLIDNMGLLCSRQRHNPADSEENEQTAEIDRRIEQETKAEKHIQKLLLLGAGDSGKSTIFKQIKLLFQSGFDEAELKGYIPVIHANVYQTIKILHDGSKELSLSLADSSKFMISDENKKIGEKLSEIGGRLDYPHLTKELAHEIETLWRDNAIQETYSRGNELQVPDCTHYFMENLQRLSDADYVPTKEDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQKNERRKWIHLFEGVTAVIFCAAISEYDQTLFEDDNKNRMMETKELFEWVLKQPCFEKTSFMLFLNKFDLFEKKVLKVPLNVCAWFKDYQPVSTGKQEIEHAYEFVKKKFEELYFQSIAPDRVDRVFKIYRTTALDQKLVKKTFKLVDETLRRRNLFEAGLL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must have high specificity towards G-protein beta/gamma-subunit complex binding. The designed protein sequence is ",
    "response#1": "SNAMALWVFFFRTKKKHPAVIYMVNLALADLLFIVWFPLKISYHLNGNNWLFGEGLCKVFVGFFYGNMYCSILFMTCLSVQRYWVVVNPIVNSKKKSEIAIGISIAIWVLILLGTIPLYLVNQTAYISNLKITTCHDVLPENVLAHDMFSYFLSLATGLFLIPAIITAIAYILMIKTLSASIIDVSTGKKRKRAIRLIIVVLSMYLICFTPSNVLIIVHYSLLKAYSQSHVYVWYITSLCLSTLNSCIDPFIYTFAGSNFRRAFCALLQC",
    "response#2": "SNAMALWVFFFRTKKKHPAVIYMVNLALADLLFIVWFPLKISYHLNGNNWLFGEGLCKVFVGFFYGNMYCSILFMTCLSVQRYWVVVNPIVNSKKKSEIAIGISIAIWVLILLGTIPLYLVNQTAYISNLKITTCHDVLPENVLAHDMFSYFLSLATGLFLIPAIITAIAYILMIKTLSASIIDVSTGKKRKRAIRLIIVVLSMYLICFTPSNVLIIVHYSLLKAYSQSHVYVWYITSLCLSTLNSCIDPFIYALFNKDFRRAFKKIIFS",
    "response#3": "SNAMALWVFFFRTKKKHPAVIYMVNLALADLLFIVWFPLKISYHLNGNNWLFGEGLCKVFVGFFYGNMYCSILFMTCLSVQRYWVVVNPIVNSKKKSEIAIGISIAIWVLILLGTIPLYLVNQTAYISNLKITTCHDVLPENVLAHDMFSYFLSLATGLFLIPAIITAIAYILMIKTLSASIIDVSTGKKRKRAIRLIIVVLSMYLICFTPSNVLIIVHYSLLKAYSQSHVYVWYITSLCLSTLNSCIDPFIYGLFSKDFRRAFKKILC"
  },
  {
    "reference": "MNVTLKHWREAASLIITGGLRDTNAVLKHNHVSSLKKPSRQTWLPKTAFDYEVLLLQRSTASSFMPDTYVFPGGLVDDSDFCSDWMDVFKLHKHKPNFGLSSVKQNPSSRPPVFLTDRTKFGSPVSGDVAFRICAIRETFEESGILLVIPNSCDINSNERFTKVIEHDQKELAKWRLKVQSSPAHFIHLCTELGCMPNIWALHEWGNWLTPVASKNIKKRRYDTAFFICCLEDKQYTEDDQKEIVSFKWLAPPEAIKLFSDKKIQLGPPQFYELSRICHFSSLRELHRFSLHRALEGCECWLPVILFTSDGGILLLPGDELYPEEPDWTGAKKPPVLTTDKKREDLRKKDSRLHRVEFGHDLTIYMNIEPKYKHIYPLLMDSKDAVDYNSQL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must be able to perform metal ion binding under specific conditions.2. The protein should have a fast turnover rate for dodecanoyl-CoA + H2O = adenosine 3',5'-bisphosphate + 2 H(+) +   S-dodecanoyl-4'-phosphopantetheine.3. The protein design should incorporate a binding pocket that specifically recognizes Mn(2+) to enhance enzymatic efficiency.4. The designed protein should contain one or more Nudix hydrolase domains that are essential for its function. The designed protein sequence is ",
    "response#1": "HWREAATVILAAGLRHAQSADSLKTGLEKATHIAHKSAFDYQVLLLKRSENSGFMPNAYVFPGGLVDASDFSSDWQEVFQSFTKAPYYGIGVAKQPPETRPPIFATDRAQLGSPIPGDVAFRICAVRETFEESGVLLAVPKHEESGIRVRTEDARDSDTQPTLTTLSGLWDRDVLSKWRSLVIGNSANFIKMCKELQCLPNIWALHEWGNWLTPIGMHGKQRRYDTAFYICCLHDTPHTLHDEKEIVHFKWSSPSEVLQSYKSKEIWIASPQFF",
    "response#2": "HWREAATLLLAAGRPGRLPRPALGRGDYEVLLLRRHSRSGFMPSAHVFPGGLVEAADFSADWLGLLPASPRCGLGPVRPPPPGSRRAPLFATDRERLGSPLPGEVAFRICAIRETFEEAGILLLVPGGGPAAGGGPPPPLPAERLPSAAELGEWRRRVQADPACFLQLCRHLGCVPNIWALQEWSNWLTPVGRAGRGGRRYDTAFYLCCLEERPPHASQDDREVTALLWSSPAEAIERFKSQEIWFAPPQFY",
    "response#3": "HWREAATVILAAGLRHAQSADSLKTGLEKATHIAHKSAFDYQVLLLKRSENSGFMPNAYVFPGGLVDASDFSSDWQEVFQSFTKAPYYGIGVAKQPPETRPPIFATDRAQLGSPIPGDVAFRICAVRETFEESGVLLAVPKHEESGIRVRTEDARDSDTQPTLTTLSGLWDRDVLSKWRSLVIGNSANFIKMCKELQCLPNIWALHEWGNWLTPIGMHGKQRRYDTAFYICCLHDTPHTLHDEKEIVHFKWSSPSEVLQSYKSKEIWIASPQFR"
  },
  {
    "reference": "MKILSKDQVYEADQATVKSEDIPSINLMERAGGYAFHYLHQRLQGQPVNIKIFCGIGNNGGDGLVIARNLIEHNYTVSVYILNYSEHRSKDFLLALEALKGMTKKWPEVLKSAEDFPEIAPEDLVVDCIFGIGINRPADGWVQQLFKKINASGAYTLSIDVPSGMAVDRILPEGEVVRPTVVLTFQTPKLVFFLPQSGKYINNWEVLDIGLDRNYLNTVKTKAQLIGKTEAQQMYRPRQKFSHKGTYGHALIVAGSYGKMGAAVLCSKACLSSGAGLVTAYATRQGLPVLQNSVPEVMVVTDRHDGHYLEELKFDLEPDVIGIGPGLGTEKPTQNAFETFLKANKTPLVLDADALNMLAKNQALLKYLPKYSVLTPHPKELERLIGEWKDDFEKLEKAEAFSKKYNCVLVCKDAHTIVVFDGNYYVNNSGNPGMATGGSGDVLTGIITGLIAQGYDALTAAIFGSYIHGRAADIAINHTGYEGLTAGVIIEFLGQAFLDLFSKPEQEKKQEIRNIEG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of Mg(2+) is necessary for the protein to function in the desired environment.2. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration3. The protein contains novel YjeF N-terminal domains that confer a unique function or activity.4. The protein design must be able to perform metal ion binding, NADPHX epimerase activity, ATP binding in order to contribute to nicotinamide nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "MRAIDRATIDEIGLPGAVLMETAGRAVAEVVRARRRAGATVAVVCGAGNNGGDGYVAARVLREWGYAASAYLAADRAKVAGDAALHLRVLEQCGGRVASIATEADLLEHERAIERADVVVDALFGTGLARDVTGHYARVVAVLNRARGFKVAVDIPSGLAADTGAVLGVAFNADATVTMAFDKPALVSSPGFARCGEVTVAEIGIPAELAHAWPEHQADTHKFRRGAVAVLAGGVAGVGAPRLSARAALRVGAGLATLLCEPEALPVHAARGPDALMQRAVADRGALEAFLRDRRVSAVLAGPALGLDRRADDLLRAVIADDRPAVLDADALTALAGLPDGGVRLLGGRGGDTVLTPHEGEFQRLFGEEPAIAAEPSKLERARKAAALSGAVIVYKGADTVVAAPDGRGAINVTGSVALATAGSGDVLAGLVAGLLAQGMPAFEAGCAAVWLHGLAGEKLGMGLIADDLPEAIPPTA",
    "response#2": "MAAADRVAIAAGTPGIALMERAGRAVAEAALRRAGAGGSVTLFCGPGNNGGDGLVAARLLRERGLRVRVVLPGAADRFVGDAAIALERWAGPVEPLNALADRQGDVFVDALFGAGLTRPLAGAFAEAVACLNGAGRPVIAVDMPSGISGDTGQVEGIAVKASETITFFRLKPGHLLQPGRSLCGPVTVADIGIPAQPADCLIAGTGDVLTGIENKLRKLREGREVRHKYQNGNVYVLGGSPGLTGAVILTAKAAWSTGCGSVQVFTTPEVSPAYDAHFVDITRKMIPGDGSGLFKADAVRAVAEVIAARPGVLVLGPGLGTHPETAAAVRQLLRLVKAPVVLDADGVRALSGHTEKLPDDLRLIVTPHPGEQEQLLGFSIAQPEQLAQALNHFPCRDNMVFLAKGSPALLATRNECRITTYDTRIFSRTGFGDVLAGLMAGMLSRLPDSTVTAESMLDVSALAMLESVMLVVGSEAYPGAAYLASAAAGRAGAGLVTVASIRAVCSLTAAKLPEPTYTLLPEAMGPNGAVIAADAAHTVHEAVKGYRALVVGCGLSNTPATRQFMANLLDSSLPAAVFDADGLNCLASHDGWWGRLPPRTVLTPHPAEMARLCGLSVEQVLADRWALARQKATEWGCTVLLKGPYTVIAEPPGWLAVLPVATSALATAGSGDVLAGVIGGLLAQGLEAFTASCVGAWLHGMAGLACAASIGEAGVVASDLLPHVLVLGGARLTGAARLCAQAAQRSGAGLVTVLAPQPVWPVYAQALQASMVQPFADAQQLGALLDDARAQVCAIGPGAGVQALTRQAVLAAAQRGKALVLDADALTVFAQQPQALFQALAANRAATAVLTPHEGEFARLFPDLAGSKPERAAQAARLAQAVVVLKGADTVIAAPDGRVLINSNAPPELATGGTGDVLAGMVAAWLAQGMPALAAACAAVWLHGQAAQRALGREG",
    "response#3": "QLENKLRKLREGREVRHKYQNGNVYVLGGSPGLTGAVILTAKAAWSTGCGSVQVFTTPEVSPAYDAHFVDITRKMIPGDGSGLFKADAVRAVAEVIAARPGVLVLGPGLGTHPETAAAVRQLLRLVKAPVVLDADGVRALSGHTEKLPDDLRLIVTPHPGEQEQLLGFSIAQPEQLAQALNHFPCRDNMVFLAKGSPALLATRNECRITTYDTRIFSRTGFGDVLAGLMAGMLSRLPDSTVTAESMLDVSALAMLESYARATCQHESAVSPSAPEAGGMN"
  },
  {
    "reference": "MVEELIALLIMASALGMDAFSIALGMGTLGLRFSQMFKVGLTIGVFHVIMPLMGMVAGKLLSAHLGLFANWLGAGLLLWLGLVMIVSPFQEKERTFVDPSGIGLFVFALSVSLDSLSAGLSLGMVGAKMALAVVAMGVMSTVLSWLGLFIGMRFQRYVGPYSELLGGFILCGFGVKLLLPY",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must be able to perform manganese ion transmembrane transporter activity under specific conditions.2. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "MTKPTTV",
    "response#2": "MPSA",
    "response#3": "EHYKV"
  },
  {
    "reference": "MCTSRQIIGSLLVLSVLEIGLGLSSVAVGAVTFFRVRMEQKPQLGDSSPVWSGACFLICGVCGIFCAKKKSGLIMILFSACCICGLIGGILNIQFLRALNKRSSSLYSLYLASMSLACIGISGCTISTWLTCRLASYEQRRMFLEREHSLHHSHEMTEKDTENITNGGGPLALNGRV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be expressed specifically in the membrane2. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "MPNTMKF",
    "response#2": "M",
    "response#3": "MSSSFKLG"
  },
  {
    "reference": "MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTVHHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYNPFADLKAMMNKKK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be optimized for cytosol-specific folding and stability, while maintaining endonuclease activity. The designed protein sequence is ",
    "response#1": "GLLLRAGQRDSLGYFATRHDKSVIFSPRGDGAVTYRLLAAVSLASADPIGPRSAWAGAIEAWLAETRRFGWFPAVLAASEEGARAYVTAGLKALPVGDEAIIEVAEFSLEGATMEPVRRAVRRVRRAGYTFTVARHGTLSAGELAEIERRAEDWRGDDTERGFSMALGRLGDPADEACVAVLARDEAGRLRGVLSLVPWGSHGLSLDLMRRDRGAENGLVEAMVAALVQAARDELGVRAISLNFAAFREVFSAAEXVGARPGARLGARLLTFASRFWQIEGLYXSNARYLPRWTPRYLCRR",
    "response#2": "GLLLRAGQRDSLGYFATRHDKSVIFSPRGDGAVTYRLLAAVSLASADPIGPRSAWAGAIEAWLAETRRFGWFPAVLAASEEGARAYVTAGLKALPVGDEAIIEVAEFSLEGATMEPVRRAVRRVRRAGYTFTVARHGTLSAGELAEIERRAEDWRGDDTERGFSMALGRLGDPADEACVAVLARDEAGRLRGVLSLVPWGSHGLSLDLMRRDRGAENGLVEAMVAALVQAARDELGVRAISLNFAAFREVFSAAEXVGARPGARLGARLLTFASRFWQIEGLYXSNARYLPRWTPRYLCT",
    "response#3": "GLLLRAGQRDSLGYFATRHDKSVIFSPRGDGAVTYRLLAAVSLASADPIGPRSAWAGAIEAWLAETRRFGWFPAVLAASEEGARAYVTAGLKALPVGDEAIIEVAEFSLEGATMEPVRRAVRRVRRAGYTFTVARHGTLSAGELAEIERRAEDWRGDDTERGFSMALGRLGDPADEACVAVLARDEAGRLRGVLSLVPWGSHGLSLDLMRRDRGAENGLVEAMVAALVQAARDELGVRAISLNFAAFREVFSAAEXVGARPGARLGARLLTFASRFWQIEGLYXSNARYLPRWTPRYLCS"
  },
  {
    "reference": "MPSLEDYRIPYPAAGPCQKRTVIYLPQQDSAVEQHHLRVQLIPGRHVRCEDGCFYQLTGTVSEETLQSWGYPYYVVTLDDIYPAQCSPSDPANPRTFVPLRESPMIPYNSKRPIVVYVPEDAEVRYRVWCSDVLQVQESQQELEAPAVSQSCPVPVRERQNNPEGHAHPVVVHSVESPEVSGHKDGDQPMKKSSKLKQSCSNSSRSLKHSASGSSPKNTPLFSRESVPPEHSLSAAQQRRRSNENSAIDETGGGASRKKRSDSTSSRKDDQDSGYEKKVKNLWNRARGNSSPKRSASPKKSGRDSRRNS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.2. The designed protein must be optimized for serine-type endopeptidase inhibitor activity. The designed protein sequence is ",
    "response#1": "VIGGDECNINEHRSLVVLFNSSGALCGGTLINQEYVLAAAHCDMPNMQILLGVHSASVLNDDEQARDPEEKYFCLSSNNDTKWDKDIMLIRLNRPVNNSVHIAPLSLPSSPPSVGSVCRIMGWGKTIPTQETHPDVPHCANIKLFHYSLCRAVYVGMPAQSRILCAGILQGGIGSCQGDSGGPLICNGQFQGIVSATSKPCAHSLMPALYIKVFDYTDWIVANM",
    "response#2": "VIGGDECNINEHRSLVVLFNSSGFLCAGTLINQEWVLTAANCDRKNIRIKLGMHSKNVTNEDEQTRVPKRSTFVSVAKTSHQTLGTRTSMLIRLKRPVNDSPHIAPLSLPSSPPSVGSVCRVMGWGTISPTKVSYPDVPHCANINLLDYEVCREAHGGLPATSRTLCAGILEGGKDSCQGDSGGPLICNGQFQGILSWGVHPCGQPHKPGVYTKVSDYSEWIQSC",
    "response#3": "VIGGDECNINEHRSLVVLFNSSGFLCAGTLINQEWVLTAANCDRKNIRIKLGMHSKNVTNEDEQTRVPKRSTFVSVAKTSHQTLGTRTSMLIRLKRPVNDSPHIAPLSLPSSPPSVGSVCRVMGWGTISPTKVSYPDVPHCANINLLDYEVCREAHGGLPATSRTLCAGILEGGKDSCQGDSGGPLICNGQFQGILSWGVHPCGQPHKPGVYTKVSDYSEWIQSIIR"
  },
  {
    "reference": "MTNIRKSHPLMKIINNAFIDLPTPSNISSWWNFGSLLGLCLIMQILTGLFLAMHYTPDTTTAFSSITHICRDVNYGWTIRYLHANGASMFFICLYTHMGRGLYYGSYIFQETWNVGMMLLITVMATAFVGYVLPWGQMSFWAATVITNLLSAIPYIGTTLVEWVWGGFSVDKATLTRFFTLHFILPFITLTLTMVHLLFLHETGSNNPTGIPSNMDKIPFHPYHTIKDTMGALLLILSLLTLTLFAPDLLGDPDNYTPANPLNTPTHIKPEWYFLFAYAILRSVPNKLGGVLALLLSILILVFIPMLHTAKQRSMMFRPFSQFLFWTLIMDLLTLTWIGGQPVEHPYVTVGQLASILYFLLILILMPTASLIENKLLKW",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.2. The a ubiquinone, heme b binding site should be located in a region of the protein that is accessible to the ligand.3. A protein that localizes to mitochondrial inner membrane, respiratory chain complex III and can perform ubiquinol-cytochrome-c reductase activity, metal ion binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "PSMSVLAEGHQWYWSYQYPDFLDSNDEFIEFDSYIVPESDLEEGALRMLEVDNRVILPELTHVRFIITAGDVIHSFAVPSLGVKCDAYPGRLNQVSVFINREGVFYGQCSEICGILHSSMPIVIESVSLEKFLTG",
    "response#2": "MSVLAEGHQWYWSYQYPDFLDSNDEFIEFDSYIVPESDLEEGALRMLEVDNRVILPELTHVRFIITAGDVIHSFAVPSLGVKCDAYPGRLNQVSVFINREGVFYGQCSEICGILHSSMPIVIESC",
    "response#3": "DPSMSVLAEGHQWYWSYQYPDFLDSNDEFIEFDSYIVPESDLEEGALRMLEVDNRVILPELTHVRFIITAGDVIHSFAVPSLGVKCDAYPGRLNQVSVFINREGVFYGQCSEICGILHSSMPIVIESVSLEKFLTWLEA"
  },
  {
    "reference": "MDGRLQIFAGTSHPALAKDITRELEVQLGRALVGTYRNGETRVRLDENVRGSDCFVIQTICMPYDHHLMELLLMIDALRRASAARITAVIPYYGYSKQEKKTAGREPISAKLVANLITVAGAHRILSIDLHAPAIEGFFDIPVDHLRATPLFARHFRDIVAGGGVVVSPDAGGVARAEDFRGRAGGTLAIISKRHPEPDQTATLEMVGDVSGKAVIVVDDMISTGETVVQAARLLRDRGAASIHVAATHGIFAGDAMRLLDASPITDIVVSDTLPLAEDVDRQRVTTITVAPLLAEAIMRIHKDLSISALFT",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein have enhanced ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-   diphosphate + AMP + H(+).2. Ideal protein should have a binding affinity for key enzymes in the Metabolic intermediate biosynthesis, 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis pathway to enhance the overall efficiency of the pathway.3. The designed protein should have a binding site that is specific to the target Mg(2+), ATP ligand.4. The designed protein should have ATP binding, kinase activity within cytoplasm to regulate phosphorylation, ribonucleoside monophosphate biosynthetic process, nucleotide biosynthetic process. The designed protein sequence is ",
    "response#1": "MKLMSGNANRPLARAIADYLDAPLSNVEIETFADGELFVRIEENMRGEDVFLIQSTSKPANNHLMELLICMDALSRASARRITAVIPYFGYARQDRKTGGRTPITAKLVANLIAKAE",
    "response#2": "MKLMSGNANRPLARAIADYLDAPLSNVEIETFADGELFVRIEENMRGEDVFLIQSTSKPANNHLMELLICMDALSRASARRITAVIPYFGYARQDRKTGGRTPITAKLVANLIAKAEKGVKQFVGGNCTVSLMLMGI",
    "response#3": "MKLMSGNANRPLARAIADYLDAPLSNVEIETFADGELFVRIEENMRGEDVFLIQSTSKPANNHLMELLICMDALSRASARRITAVIPYFGYARQDRKTGGRTPITAKLVANLIAKAEELAQAA"
  },
  {
    "reference": "MVNVVVTGAAGRMGTQIVRLVAAAEGLKLTGAVERPGHAGQDAGALAGIPPLGVAVVDDLAGALAGADVVIDFTSHEASARNAELCAEKGVALVIGSTGFTPEAKARVAAAAARIPVVFSPNMSVGVNVLFELVRQAAKVLGDAYDVEIVEIHHKRKRDAPSGTAVALGEVAAGALGRDPADALAYTRHGILGERPPWQIGVQTLRGGDVVGEHTVYFCGEGERLELTHRATSREQFARGAVRAAGFIAGKPAGLYDMADVLGLRSAR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.2. The designed protein should have a high specificity for NADP(+), (S)-2,3,4,5-tetrahydrodipicolinate, NAD(+) binding, with minimal off-target effects.3. The protein design should take into account the physiological conditions of the (S)-tetrahydrodipicolinate from L-aspartate: step 4/4, L-lysine biosynthesis via DAP pathway pathway.4. The design of the protein must focus on the precise arrangement of amino acids in the Proton donor/acceptor to enable effective catalysis of (S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-   4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH.5. A protein that contributes to lysine biosynthetic process via diaminopimelate by functioning in cytoplasm with NAD binding, 4-hydroxy-tetrahydrodipicolinate reductase, oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor, NADP binding. The designed protein sequence is ",
    "response#1": "MNIALLGYGRMGQTIEEISKNRDHKIVFKLDDDIENHDLNDFEIDVAIDFSVPKAAFKNITTCFKHAIPVVSGTTGWLDDYAKAREICKKEDSAFIYASNFALGAVLMMHFAEQAARFYESAEIVELHHPNKVDAPSGTARTTATKIAAARQEAGLGEVPDATKSQLNGARGAVVEGVHVHSVRLRGLVAHQEVLFGAEGETLTIRHDSMDRVSFMSGVLTGVRGVLDRPGLTL",
    "response#2": "MRVGVLGARGKVGATMVAGVEAADDLTFTTGVDAGDSLSALVDSDTEVVIDFTHPSVVMDNLKFLIDNGIHAVVGTTGFTDERLDQVREWLAATPGASVLIAPNFAIGAVLMMQFSKWAAKYFPDVEIIEKHHDQKLDAPSGTAVKTAQLIEEVRDSKVQGHPEEKETIPGARGANEDGMRIHSVRLPGLVAHQEVIFGGTGETLTIKHDSHHRQSFMSGVKLAVDQVQRIHTLVYGLENLL",
    "response#3": "MRVAVSGAAGRMGRLVVKNAVAEGLKVVQAFDINEVGKDAGELAGVGKIGVPIEDDISKLDADVLIDFTTAEAAMKNAEVAAEKGVRVVMGTTGFTDEDRKRLAELAEKVPMIVSPNFAIGAVLMMQFSKWAAKYFPDVEIIEKHHDQKLDAPSGTAVKTAQLIEEVRDSKVQGHPEEKETIPGARGANEDGMRIHSVRLPGLVAHQEVIFGGTGETLTIKHDSHHRQSFMSGVKLAVDQVQRIHTLVYGLENLL"
  },
  {
    "reference": "MTSLLLFGASEGGLFDFDATLPLMAAQVVLLTFILNALFFKPVGRVVEDREDYVLTSRAEAKKKLAEVEKLENDLKNQLKEARKAAQQVISEAEEDSEKLYKEALNLANSEANASREQARREIDSQRDSALKKLKSDSDKLGDLIVERLLAAK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein can be targeted to plasma membrane-derived thylakoid membrane, proton-transporting ATP synthase complex, coupling factor F(o) and perform proton-transporting ATP synthase activity, rotational mechanism.2. The protein must exhibit the following characteristics: The b'-subunit is a diverged and duplicated form of b found in plants and photosynthetic bacteria.; Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0).3. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "RGSKAGLFGGAGVGKTVLITEMIHNIVSRYDGVSIFCGIGERSREGEELYREMKAAGVLNNTVMVFGQMNEPPGSRFRVGHAALTMAEYFRDQAHRDVLLMIDNVFRFIQAGSEVSGLMGQLPSRVGYQPTLATELAELEERICSTAHGAITSVQAVYIPADDLTDPAAVHTFSHLSASIVLSRKR",
    "response#2": "RGSKAGLFGGAGVGKTVLITEMIHNIVSRYDGVSIFCGIGERSREGEELYREMKAAGVLNNTVMVFGQMNEPPGSRFRVGHAALTMAEYFRDQAHRDVLLMIDNVFRFIQAGSEVSGLMGQLPSRVGYQPTLATELAELEERICSTAHGAITSVQAVYIPADDLTDPAAVHTFSHLSASIVLSRKR",
    "response#3": "RGSKAGLFGGAGVGKTVLITEMIHNIVSRYDGVSIFCGIGERSREGEELYREMKAAGVLNNTVMVFGQMNEPPGSRFRVGHAALTMAEYFRDQAHRDVLLMIDNVFRFIQAGSEVSGLMGQLPSRVGYQPTLATELAELEERICSTAHGAITSVQAVYIPADDLTDPAAVHTFSHLSASIVLSRKR"
  },
  {
    "reference": "MADRAAIILAAGQGTRMQSKLPKVLHPVGGRAMLDWLIALAEDLGCQRIIVVASPGTPEVQAHVRDTLGDQALVLQETALGTGHAVRCAETALAGFKGDVVVLYGDTPLIPSSVVEALFEQISSTSELGVLGFNAADPGAYGRLVTGSDGMLQAIVEAKEASADELAISFCNSGVMAAKAGLLFELLAAVTNDNVKGEYYLTDTVGLARARGAACCAVNCEETDVLGVNSRVELAQAEAAFQQRRREQAIRDGVTMIAPETVFFSYDTEIQTDTFIEPNVVFGTGVRIASNVRIGAFSHIQQAVISEDCEIGPFARLRPGSVMSAGAKIGNFVEMKNTQLGPGAKANHLAYLGDSEIGAESNIGAGTIFCNYDGFRKHRTIVGAGAFVGSNSSLVAPVRIGDGAMIGSGSVVTQDVEPNALALGRAQQTVKSGWASRFRDAMTAIKTARSKR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.2. The protein should be optimized for stability and expression in cytoplasm where it will participate in cell morphogenesis, UDP-N-acetylglucosamine biosynthetic process, lipid A biosynthetic process, peptidoglycan biosynthetic process.3. The protein should have a high affinity for acetyl-CoA.4. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain..5. We require a protein that can specifically bind to the metabolites produced in the Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MKAIVLAAGKGTRMKSDLHKVLHPIAGRPMLSHLLATVDTLGPATSLVVVGSGREQVEKHVQPHTQVVVQEPQLGTAHAVQQAQTALGGFEGDVLVLYGDVPLVTAETMARMLERLNALDAPAAVVLGFRPADPLAILSARLDDGRGYGRIVREGGAVAAMVEHKDADAAQRAIGLCNSGVVAADAQTLRGWLARIENRNAQGEYYLTDIVALARADGLSAHATEAPEAEAVGVNTRAELAAAEAAFQAARRA",
    "response#2": "MAGVVVLAAGAGTRMRSKTPKVLHPLAGRSLLAHALHAAAEIDPQHLITVVGHDREQVSGAVAEIGTDLRRDVSLAVQETQLGTGHAVQCALTVLPADFTGDVLVTSADVPLLDGHTLAALLDEHRSYPGRSGATVLTFSPPDPNGYGRIVRDADGRVSAIVEHADATPEQARITEVNSGVYAFDAVLLRDAIVGLSDHNAQHELYLTDVIGLARAAGDALGNLRVLGEAERAELARAEALFQRRSRAEAQENGVTMVDPETVYFALDTYLGRDVTVGPHVVFGPEVTVESGAAIEAFCHLEGCHISRGATIGPFARLRPGAELAEDVHVGNFVEVKNSILGEGVKVGHLTYLGDADVGEGTNIGAGTVTCNYDGVFKHRTTIGAHAFIGSDTMLVAPVTVGDGALTGSGSTITQDVPPGALALGRARQSNLEGWVRSRRKSG",
    "response#3": "VIILAAGKGTRMKSNKPKVLQTLANKPLLAHVLDTCGTMAVNKTIVVHGFGGWQVQSAMADYDINWVEQTEQLGTGHAVKVTLNDLPQAGKSLILYGDVPLVNADTLTKLKQAETHNIALLTVALDNPTGYGRIVRDAEGNICGIVEEKDATDAQKQITEINTGIMVANNKLLKQWLAQLDNNNAQQEYYLTDIIAMAVKNGVKIVTAQPAHLWEVEGVNNKLQLASLEREYQKQCAENLMVNN"
  },
  {
    "reference": "MNETTDFGFREVPRGQKASLVRQVFDSVAPRYDLMNDLMSLGIHRIWKRIFVNAIAASPRETLLDLAGGTGDIAFGALERGAGHVILTDVNAEMLEVGRRRALDRGIVDRISFAVVDAERIPLPDRSVEKASIAFGLRNCTDKDAVLREARRVLRPGGRFHCLEFSKLEVAALEKIYETWNFRVLPEIGARVAKDRASYEYLAESIAKFPDQVTLAGMMERAGLERVAYRNLSGGIVAIHTGWRL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein with 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity is needed to enhance menaquinone biosynthetic process, aerobic respiration in a specific context. The designed protein sequence is ",
    "response#1": "GCCCL",
    "response#2": "LVEL",
    "response#3": "MY"
  },
  {
    "reference": "MSDYDETALVCDNGSGLVKAGFAGDDTPRAVFHAIVGRPRHQDDMDDMGKKGYYVGDEAQSKRDILSLKHPIERGIITNWDDMEKIWHHTFYNELCVAPEEHPTLLTEAPLNPKANREKMTQIMLETFNMPAMYVSIQAVLSLYASGRTTGIVLDSGEGVTHAVPIAEGYALPPAIMRLNLAGRDLTDYLMKILNERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKRYELPDGQVITIGNERFCCPETLFQPSFMGMESAGIHEITHSSIMKCDIEIRKDLYANNVLTGGATLFPGIADRMQKEITALAPSTMKIQIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYEEIGPSIIHCKCF",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a low Km value for ATP + H2O = ADP + H(+) + phosphate.2. The protein must exhibit the following characteristics: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells..3. The protein can be expressed in cytoplasm and perform hydrolase activity without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "MSFGDIGLLNHTNIPCVLDFLKHRYLKNQIYTTAVPLIVAINPYKDLGNTTNEWIRRYRDTADHTKLPPHVFTCAREALSNLHGVNKSQTIIVSGESGAGKTEATKQIMRYFASSKSGNMDLRIQTAIMAANPVLEAFGNAKTIRNNNSSRFGRFMQLVISHEGGIRYGSVVAFLLEKSRIITQDDNERSYHIFYQFLKGANSTMKSKFGLKGVTEYKLLNPNSTEVSGVDDVKDFEEVIESLKNMELSESDIEVIFSIVAGILTLGNVRLIEKQEAGLSDAAAIMDEDMGVFNKACELMYLDPELIKREILIKVTVAGGTKIEGRWNKNDAEVLKSSLCKAMYEKLFLWIIRHLNSRIEPEGGFKTFMGMLDIFGFEVFKNNSLEQLFINITNEMLQKNFVDIVFERESKLYKDEGISTAELKYTSNKEVINVLCEKGKSVLSYLEDQCLAPGGTDEKFVSSCATNLKENNKFTPAKVASNKNFIIQHTIGPIQYCAESFLLKNKDVLRGDLVEVIKDSPNPIVQQLFEGQVIEKGKIAKGSLIGSQFLNQLTSLMNLINSTEPHFIRCIKPNENKKPLEWCEPKILIQLHALSILEALVLRQLGYSYRRTFEEFLYQYKFVDIAAAEDSSVENQNKCVNILKLSGLSESMYKIGKSMVFLKQEGAKILTYVGEEAQAKRL",
    "response#2": "SDMTLLSKVTNEAINDNLQKRFQNAEIYTYIGNVLISVNPFRDLGIYTEDILQSYRGKNRLEMTPHVFAIAEGAYYNMNAYKENQCVIISGESGAGKTEAAKRIMQYIAAVSGGSNSGIQDVKDMVLATNPLLESFGCAKTLRNNNSSRHGKYLEIMFNAHGEPIGANITNYLLEKNRVVQQIHDERNFHIFYQFTKAATATHRENYGIQGPEAYAYTANSQCLDVNGIDDHADFRETISAMNTIGLTADEQDNIFRMIAAILWIGNVQYVENQEGNAEISDPGVPDFVAYLLEVDAGNVTKALTQRIMETQRGGRRGSVYEVPLNPTQAAAARDALAKAIYNNMFDWIVERINQSMNPRTQSSNVIGVLDIYGFEIFDNNSFEQLCINYVNEKLQQIFIELTLKKEQEEYAYEQIQWTPIKYFNNKIVCDLIEEKRPPGIFSALNDACATAHADPTAADNSFIQRTGMLSSNPHFDSRGTKFLIKHYAGDVMYNVQGMTDKNKDSLLKDILDLVDSSTNSYLQKLFPDRPDPNSKKRPPTAGDRIKASANALVENLMRAQPSYIRTIKPNQNKSPTEYDSQAILHQIKYLGLQENIRVRRAGFAYRNTFEKMVERFYLLSPNTSYAGEYTWQGDARSGCERILTDTGIAREEWQMGVTKAFIYVGDEAQSKRM",
    "response#3": "MSFGDIGLLNHTNIPCVLDFLKHRYLKNQIYTTAVPLIVAINPYKDLGNTTNEWIRRYRDTADHTKLPPHVFTCAREALSNLHGVNKSQTIIVSGESGAGKTEATKQIMRYFASSKSGNMDLRIQTAIMAANPVLEAFGNAKTIRNNNSSRFGRFMQLVISHEGGIRYGSVVAFLLEKSRIITQDDNERSYHIFYQFLKGANSTMKSKFGLKGVTEYKLLNPNSTEVSGVDDVKDFEEVIESLKNMELSESDIEVIFSIVAGILTLGNVRLIEKQEAGLSDAAAIMDEDMGVFNKACELMYLDPELIKREILIKVTVAGGTKIEGRWNKNDAEVLKSSLCKAMYEKLFLWIIRHLNSRIEPEGGFKTFMGMLDIFGFEVFKNNSLEQLFINITNEMLQKNFVDIVFERESKLYKDEGISTAELKYTSNKEVINVLCEKGKSVLSYLEDQCLAPGGTDEKFVSSCATNLKENNKFTPAKVASNKNFIIQHTIGPIQYCAESFLLKNKDVLRGDLVEVIKDSPNPIVQQLFEGQVIEKGKIAKGSLIGSQFLNQLTSLMNLINSTEPHFIRCIKPNENKKPLEWCEPKILIQLHALSILEALVLRQLGYSYRRTFEEFLYQYKFVDIAAAEDSSVENQNKCVNILKLSGLSESMYKIGKSMVFLKQEGAKILTYVGDEAQSKRV"
  },
  {
    "reference": "MSQIIDQVQAALQNIDKELEKYPALKELEKQIPVPKSYILLGFVGFYFILIFLNIGGIGQLLSNIAGLVIPGYYSLLALETPGKADDTQYLTYWVVFATLNVFEFWSKAILYWVPFYYLFKTAFLLYIGLPQYGGAELVYKAIVKPLAQKLVNIQPHGGPSDSLKAQAQSAVDAAESHVPQGHSTGVSH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Required to generate and maintain the structure of the tubular endoplasmic reticulum network and the vacuole2. The protein should be optimized for stability and solubility when expressed in cortical endoplasmic reticulum, endoplasmic reticulum membrane, nuclear envelope, cell division site where it will be involved in endoplasmic reticulum tubular network membrane organization. The designed protein sequence is ",
    "response#1": "FATIGVGGKLVIIKPAGTIDSITGHVLSTSSVHVDDLKTFLHFDEQSGKVIESVQNFKGPLIAGQTPTHSVRLYIQRQIDALRQIRNAGDVKKSEVVDALLVWQLLEIMVQQHR",
    "response#2": "FATIGVGGKLVIIKPAGTIDSITGHVLSTSSVHVDDLKTFLHFDEQSGKVIESVQNFKGPLIAGQTPTHSVRLYIQRQIDALRQIRNAGDVKKSEVVDALLVWQLLEIMVQQHH",
    "response#3": "FATIGVGGKLVIIKPAGTIDSITGHVLSTSSVHVDDLKTFLHFDEQSGKVIESVQNFKGPLIAGQTPTHSVRLYIQRQIDALRQIRNAGDVKKSEVVDALLVWQLLEIMVQQHR"
  },
  {
    "reference": "MNLRNVAIWLGVVFVLIMAYSVLQRGGPTGRAGEVSYSQLLKNIDAGQVKSAEINGQQVKIASPDGKTTYTVNAPPNSEDLIKRLEARNAEIKIQDGGRFSLVNLFLNMLPILLLIGVWIFFMRQMQGGTKGAMGFGKSKARLLTENKNRVLFDDVAGVDEAKEELQEVVDFLKDPAKFQRLGGKIPKGALLVGPPGTGKTLIARAVAGEAGVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLNQLLVEMDGFEANEGIILIAATNRPDVLDPALLRPGRFDRQVVVPNPDVAGREKILRVHMKNVPLAADVEVKTLARGTPGFSGADLANLVNEAALTAARKNRRMVTMHDFEYAKDKVMMGAERRSMAMSEDEKKLTAYHEGGHALVALNVPVADPVHKATIVPRGRALGMVMQLPEGDRYSMSYDMMTSRLAIMMAGRVAEELIFGKEKITSGASSDISAATNLARNMVTRWGFSDDLGTVAYGDNQEEVFLGHSVARTQNVSPDTMVKIDAEVRRLVKGGEDDARRILTEKLEDLHSVAKALLEFETLSGEEIIGVTKGVAPKRDEPEEKKPTGPVASVPVSPSGVTA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein contains novel AAA+ ATPase domains that confer a unique function or activity.2. The protein should have Helical domains to ensure proper insertion into the membrane.3. The protein must exhibit the following characteristics: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins; Plays a role in the quality control of integral membrane proteins..4. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects.5. The protein requires Zn(2+) to activate a critical catalytic site.6. A protein with zinc ion binding, ATP binding, metalloendopeptidase activity functions is required to enable proteolysis. The designed protein sequence is ",
    "response#1": "APKGVLLEGAPGTGKTLLARATAGEAGVPFFSASASEFIEMIVGVGASRVRELFGEARKVAPAIIFIDEIDTIGRARGGARSIGGHDEGEQTLNQILTEMDGFSGTEGVVTLAATNRADVLDPALLRPGRFDRTITVNAPTLEERVAILQSKADL",
    "response#2": "LLEGPPGTGKTLLARAIAGEAGVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIVFIDEIDAVGRHRGAGYGGGNDEREQTLNQLLVEMDGFEANEGVIIIAATNRKDVLDPALLRPGRFDRQITVHFNTVENATGIIDRALRGESL",
    "response#3": "IPKGVLLVGPPGTGKTLLARAIAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLNQLLVEMDGFEPNESIILIAATNRPDVLDPALLRPGRFDRQVVVPNPGIDDMVDVDIRVIARGTPGFSGADLSNLVNEAALLAARKGKRVVTQIDFEGADRILEGVREGR"
  },
  {
    "reference": "MSLFIGTHNIKNSAIQLLKTKLKNEFLKGVKCITKYPENAHSIFKVQEYTGYKGVTFIVSTKWNLTVDSIHKLIPEIKSSPSACLLLVNQPYPVIEKITDILTKHGITNANLLEFSKSSLLHPFKRNRHLLSFVPYPMTAETLGDISWSFSRRENMKFEKDQQYILSKFKSPRNLLIPYPSFLIQKFLDMAILPPMAALRILFYQYSKASTMSLTDDFYNKYVLENLNIINNLQIFLHLDLEPLHIKSLAKARKRFYETDLKTTFLNAFSRDELLMLVNYFIFRGTELKLSVSMNLAFRDILLSSAYLNKTK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a specific function within the mitochondrion. The designed protein sequence is ",
    "response#1": "MSFRVKGWSDQFSESFYERATTLLTAALNKGTKHSMIADHITVKELDLGPQPPQLEILEVGDLAPDRFQGLFNLHYSGDASLVLQTKIRANPLSVQKSNVPSFSSRNTMLASRAPLTVPMFLRLSDLKLNGIVVLVFSKQKGITVVFRNDPLESVRVSSSFDSIPAIARFLQREIEVQLVSLFQEELPSIIYKMSTL",
    "response#2": "SLYNVDGGHRAVMFNRLTGIKEKVYPEGTHFMVPWFERPIIYDVRARPYLVESTTGSHDLQMVKIGLRVLTRPMGDRLPQIYRTLGENYSERVLPSIIHETLKAVVAQYNASQLITQREAVSREIRKILTERASNFDIALDDVSITTLTFGKEFTAAIEAKC",
    "response#3": "MSFRVKGWSDQFSESFYERATTLLTAALNKGTKHSMIADHITVKELDLGPQPPQLEILEVGDLAPDRFQGLFNLHYSGDASLVLQTKIRANPLSVQKSNVPSFSSRNTMLASRAPLTVPMFLRLSDLKLNGIVVLVFSKQKGITVVFRNDPLESVRVSSSFDSIPAIARFLQREIEVQLVSLFQEELPSIIYKMSL"
  },
  {
    "reference": "MWVRTTITVKRWTEERSGRKIERTERTDITRLPSWKRGYPASVDSSSDLFSFSEGENKETDRRCWKHQHCPGHTIHPFSDCGHHNRCMHAVSQCDCESRCRSHRPVSVAIIYHPTHHMYMTDDDLEENWVSRKNHLSPSARPPDPNTGSATEVPDLSVPITIWRSESPIEKCQESNVIKDIKRKEKEQDEEEMVDEKANLKKKAKGKLTKKKTPVKSESSPADLSQSVRGPVRTPESSPESPGGLESEYSCERGKERPSSEDVVESLSPRKKEKTSSGQAKKNGTKKETQKTSKRKKSSPVPNPNLS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should exhibit Basic and acidic residues, Polar residues compositional bias.2. The designed protein should have phospholipase A2 activity within mitochondrion to regulate phospholipid metabolic process, mitochondrial electron transport, cytochrome c to oxygen. The designed protein sequence is ",
    "response#1": "LKFKVENFFCMGSPLAVFLALRGIRPGNTGSQDHILPREICNRLLNIFHPTDPVAYRLEPLILKHYSNISPVQIHWYNTSNPLPYEHMKPSFLNPAKEPTSVSENEGISTIPSPVTSPVLSRRHYGESITNIGKASILGAASIGKGLGGMLFSC",
    "response#2": "LKFKVENFFCMGSPLAVFLALRGIRPGNTGSQDHILPREICNRLLNIFHPTDPVAYRLEPLILKHYSNISPVQIHWYNTSNPLPYEHMKPSFLNPAKEPTSVSENEGISTIPSPVTSPVLSRRHYGESITNIGKASILGAASIGKGLGGMLFSRFGC",
    "response#3": "LKFKVENFFCMGSPLAVFLALRGIRPGNTGSQDHILPREICNRLLNIFHPTDPVAYRLEPLILKHYSNISPVQIHWYNTSNPLPYEHMKPSFLNPAKEPTSVSENEGISTIPSPVTSPVLSRRHYGESITNIGKASILGAASIGKGLGGMLFSRFGRR"
  },
  {
    "reference": "MRITLLRMNQEILSVEQMYRADALALAAGIAGARLMEAAGWAVARAARRLLPKGRIAILCGPGNNGGDGFVAARLLARDGWSVRVALLGAVERLKGDAAIMAGRWTGRVEPLTPDALHGCVGVIDALFGAGLARPLEGAAKAVVEEIGRLRLPVIAVDVPSGVHGDSGAVLGLAPDCLATVTFFRPKPGHCLLPGRLKCGELVVADIGIPAAVLDEIAPDLCRNGPGVWALPEIKAEGHKYSRGHVLILGGAEMTGAARLAARAARRVGAGLVSIAAPPEALGVYRAGDPGVLVASSEPFADLLEDGRRNALLLGPGGGVGEALAGKVLAALGTGRPCVLDADALSSFEGRSADLFRHLSDRVVLTPHDGEFRRLFGEMPGSRLERARHAARLSGAIVLLKGADTVVAHPKGGATITTDAPPWLATAGSGDVLAGLLAGLLAQGMEPFEAACAAVWLHGRAAAEFGPGLIAEDLSEAIPAILAERRWDPHD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a low Km value for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.2. The designed protein should have a binding site that is specific to the target (6S)-NADPHX, K(+), AMP ligand.3. A protein with ATP binding, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, NADHX epimerase activity, metal ion binding functions is required to enable nicotinamide nucleotide metabolic process.4. The protein contains novel YjeF N-terminal domains that confer a unique function or activity.5. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration6. The protein must bind to Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MRAMDAQTCKLHAMTGLALMERAGRAIFSKLSTTKPWCETRPLIVCGPGNNGGDGLVVAEALLLAGMDVSVCLVSEDQHLSTENRQVYERLKDRVHRIQRSEDIEWLKQQISCHDWILEALFGIGLTRPIEGVFKAVVALLNASGKSIVSIDLPAGLHTDSGQVLGMAVNATETWAIQAFKFGHFLADGKDYCGRLDCVDVGIVPVETPADVVARFPPHLLIVGGDSGTAGAIRMAGEAALRAGAGLVRVLTRAENIGPLLTARPELMVHELTDAALEQGLEWADVLVIGPGLGQEKWGKRALQKAENFRKPMLWDADALNLLAIHPDKRHNRVITPHPGEAARLLRCSVAEIESDRLLSARRLAQQYGGVVVLKGAGTVVADETQLGLIDAGNAGMGSGGMGDVLSGITGAMLGQKLEAYDAACAACVAHGAAADAVASRIGTRGMLATDLFTTP",
    "response#2": "MKMLERKTIEDFGISNLVLMERAGVSTVEVLWRELGNLSQYSFLVFCGPGNNGGDGLVIARELLNFTEAVKVVLISDLTTNESRKNLEIYKSFGGEIIKISKENILDVPKLLENSNVVIDAIFGTGLSRDVKGIYAEVIDYINLYSKFTVSVDIPSGVSADTANVLGTAIRSDLTVTFEYPKVGHFLFPGRALTGKLKVVKIGMPKILSDEEMILNNIRVRGRYEHKGNFGRVTIFAGSNGYSGAAYIATEAAVKCGSGLVTLCTAEELQAILSSKLVEAMTVSVDNKNRIEELISKSDAVAVGPGMGNTKETFKTLESIISKVSCPIVIDADGLNVLKDKLNILKLKKNKIILTPHPGEFSRISGITVKDIEQDRINISKKFARENDVILVLKGYHTVVTDGESVYVNSTGNSAMASGGMGDCLTGMIVSLIGQGYSPIMAAVCGTFIHGYCGDILSEKMSNVTASDIIYAIPYCIKNIYS",
    "response#3": "MQELDRQTIQEHGIPGLELMEHAGHACMEAIFHEFGYTGGKRAVIFAGRGNNGGDGYVIARLLLQSGWQVRVCILAERERIGGDAAINLEQLPPAAVSYCTVPGQLAGLLQEELSQTDVIVDALFGTGLNSEVAGINREAVELINAAGCPILAVDIPSGIHGTSGRVLGCAVRATVTVTFAAAKLGHVLYPGADHTGRLVVADIGIPAELVDHKGRRGHALVIAGSPGKWGAGRMAARAALRAGAGLVTLAGPRADAAAPDEVMTAAIDPEAAGFDVALAELLAGKRAVAIGPGMPATAAGAALVRAVLERAEVPVVIDADALNHMAGDLAAVAAARAPVVLTPHPGEAGRLLGCSAADIERDRIAAVRRLAERTRAVVVLKGARTLVCDGAVDGFTTVNPTGNAAMATAGAGDVLTGVIVALAAQGAPPADAARAGVFLHGLAGDRAARGRIDAGLMAGDLIDELPPDAHKYSRGMVAVIAGAMPGAGLLTAIAALRSGAGYVVVLGGNGGGPHAIVHRPLDAMTIAEPRIGAVVAGPGLGREAVALDKLALAFASRHPLVIDGDAIRLVTVDQLLAHDRPMILTPHAGEFAALFGELRGSKIDQARLAARQSGAVVVYKGADTVFAAPDGQVFVAEAANSWLSTAGTGDVLAGAIAAMLASGLSPVDAAAAGVWLHADAARRCPDAHKYSRGMVAVIAGAMPGAGLLTAIAALRSGAGYVVVLGGNGGGPHAIVHRPLDAMTIAEPRIGAVVAGPGLGREAVALDKLALAFASRHPLVIDGDAIRLVTVDQLLAHDRPMILTPHAGEFAALFGELRGSKIDQARLAARQSGAVVVYKGADTVFAAPDGQVFVAEAANSWLSTAGTGDVLAGAIAAMLASGLSPVDAAAAGVWLHADAARRCEGAFIADDLAFALSA"
  },
  {
    "reference": "MHTSEMLKHVYDINLSYLLLAQRLISQDKPSAMFRLGISEEMATTLGGLTLPQMVKLAETNQLVCQFRFDSHQTITRLTQDSRVDDLQQIHTGILLSTRLLTEVSQTDEVARKKRA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have DNA binding to facilitate regulation of bacterial-type flagellum assembly, positive regulation of DNA-templated transcription.2. For general function, the protein need meet that Also regulates genes whose products function in a variety of physiological pathways. The designed protein sequence is ",
    "response#1": "RHNEFSIGASASLWECMLNAWLGRLYQLQEPQSGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQLLGHFTLALYCSSPARKKSELNYLRLEWGPDFQQHETGLIAADEVPVLTTSSAELARQQLSALNGCSWLPVNWANEKGGLHTVADSATLSRPLYAIWLQNR",
    "response#2": "RHNEFSIGASASLWECMLNAWLGRLYQLQEPQSGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQLLGHFTLALYCSSPARKKSELNYLRLEWGPDFQQHETGLIAADEVPVLTTSSAELARQQLSALNGCSWLPVNWANEKGGLHTVADSATLSRPLYAIWLQNR",
    "response#3": "RHNEFSIGASASLWECMLNAWLGRLYQLQEPQSGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQLLGHFTLALYCSSPARKKSELNYLRLEWGPDFQQHETGLIAADEVPVLTTSSAELARQQLSALNGCSWLPVNWANEKGGLHTVADSATLSRPLYAIWLQNR"
  },
  {
    "reference": "MDSSIPDPEADLIQLNGSYHTETSPCVIESRVMILLSIIIAFFGLAGNAMVLWLLAFRMRRNVFSVYILNLAGANFLFLCTHTAFSLEKIIVLFHSVHIHIPLLFYTLSTLAYLAGVSMVTAISAEYWLSGIWPYWYQGQRLKHTTTVICTGLWGLALLLSLLEGKSCGFLGSTYNQDVCWKLDFIIVAWLLVLFAVLSRSIQVLLVRIFCGSQRTPVTKLHVTIVLTALVLVICGFPFGIIFYLLYWTTEVYYIMPCNSFHETILLLSYINSCANPIICLLVGSIRHCRFQCGTLRLLLHRAMQDIPEEEGEELEEVVGERVQNSIP",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Probably involved in the function of nociceptive neurons2. The protein should contain a Helical; Name=6, Helical; Name=4, Helical; Name=5, Helical; Name=7, Helical; Name=1 membrane-spanning region. The designed protein sequence is ",
    "response#1": "MLVFVSMAGWMFGADTGSIGGITNMRDFQSRYADRYDPVTDTYSYSSARQGLLVGMVNTGTTVGCLLSSPLGDRFGKRKCIMGWTLVYITGVIVQLTTIPSWVQMMVAKIWTGLGIGALSVIAPGYQSESSPPHIRGAIVTTYQLFITLGIFIAACINMGTHKYTTHPEAQWRVPIGINLLWGILMFFGMLFLPESPRYLAVKGRNEECMKILTRNAGLPADHPIMQKEYNAIQADVEAELAGGPCSWPQIFSNEIRYRTLLGMGVMAFQQLTGNNYFFYYGTQVFRGTGLNSPFLAALILDAVNFGCTFGAIFVLEYFGRRGPLIVGGVWQSICFFIYASVGDRALTRPNGTSNHRAGAVMIVFSCLFIFSFAQTWAPAAYVIVGESYPIRYRSKCAAVATASNWFWNFMISFFTPFISNSIGFKYGYVFAACNLCAAIIIFLFAKETKTQAIQSMPSEKK",
    "response#2": "MLVFVSMAGWMFGADTGSIGGITNMRDFQSRYADRYDPVTDTYSYSSARQGLLVGMVNTGTTVGCLLSSPLGDRFGKRKCIMGWTLVYITGVIVQLTTIPSWVQMMVAKIWTGLGIGALSVIAPGYQSESSPPHIRGAIVTTYQLFITLGIFIAACINMGTHKYTTHPEAQWRVPIGINLLWGILMFFGMLFLPESPRYLAVKGRNEECMKILTRNAGLPADHPIMQKEYNAIQADVEAELAGGPCSWPQIFSNEIRYRTLLGMGVMAFQQLTGNNYFFYYGTQVFRGTGLNSPFLAALILDAVNFGCTFGAIFVLEYFGRRGPLIVGGVWQSICFFIYASVGDRALTRPNGTSNHRAGAVMIVFSCLFIFSFAQTWAPAAYVIVGESYPIRYRSKCAAVATASNWFWNFMISFFTPFISNSIGFKYGYVFAACNLCAAIIIFLFAKETKSQTLQEVEAAFR",
    "response#3": "MLVFVSMAGWMFGADTGSIGGITNMRDFQSRYADRYDPVTDTYSYSSARQGLLVGMVNTGTTVGCLLSSPLGDRFGKRKCIMGWTLVYITGVIVQLTTIPSWVQMMVAKIWTGLGIGALSVIAPGYQSESSPPHIRGAIVTTYQLFITLGIFIAACINMGTHKYTTHPEAQWRVPIGINLLWGILMFFGMLFLPESPRYLAVKGRNEECMKILTRNAGLPADHPIMQKEYNAIQADVEAELAGGPCSWPQIFSNEIRYRTLLGMGVMAFQQLTGNNYFFYYGTQVFRGTGLNSPFLAALILDAVNFGCTFGAIFVLEYFGRRGPLIVGGVWQSICFFIYASVGDRALTRPNGTSNHRAGAVMIVFSCLFIFSFAQTWAPAAYVIVGESYPIRYRSKCAAVATASNWFWNFMISFFTPFISNSIGFKYGYVFAACNLCAAIIIFLFAKETKSQTLQ"
  },
  {
    "reference": "MGGKWSKSSIVGWPEVRERMRRARGPPAATGVGAVSRDLDKRGAVTSSNINHPDCVWLEAQEEEEVGFPVMPQVPLRPMTYKGAFDLGYFLKEKGGLDGLIYSRRRQEILDLWVYHTQGFFPDWQNYTPGPGTRYPLTFGWCFKLVPVDPDEVEKATEGENNSLLHPICQHGMDDEEREVLKWQFDSRLALKHIARELHPEYYKDC",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Incorporating a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Dileucine internalization motif; necessary for CD4 internalization, Diacidic; necessary for CD4 internalization motif is critical for the successful design of the protein.2. A protein that can perform GTP binding, SH3 domain binding in host cell plasma membrane, membrane, virion component, extracellular region, host cell Golgi membrane to support suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host autophagy.3. For general function, the protein need meet that Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPAVRERIRRTDPAAEGVGAVSRDLEKHGAITSSNTAATNADCAWLEAQAQGEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRFPITFGWCFKLVPVDPDEVEEANKGENNSLLHPICQHGMDDEHKEVLQWKFDSHLAFHHRAQELHPEYYKDC",
    "response#2": "MGGKWSKSSIVGWPQVRERIRQTPPAAEGVGAVSRDLEKYGAITSSNTAANNADCAWLEAQEEEGEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSRKRQEILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEQEKVEEATEGENNSLLHPICQHGMDDEEKEVLKWKFDSHLAHRHMARELHPEFYKDC",
    "response#3": "MGGKWSKSSIVGWPAIRERIRRTEPAAEGVGAASQDLDKYGALTSSNTPATNADCAWLEAQEEEEVGFPVRPQVPLRPMTFKGAFDLSFFLKEKGGLDGLIYSKKRQEILDLWVYNTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVDPREVEEANEGENNCLLHPMSQHGMEDEDREVLMWKFDSNLARRHIARELRPEYYKDC"
  },
  {
    "reference": "MAATGGRRCRRLEGETARAVLANVDTLLFDCDGVLWRGEAALSGAPAALGRLAAAGKRLCYVTNNSSRTRVAYTEKLRRLGFPPAEPRHVFGSAFCAARYLRQALPPGAAAYVLGGPALSAELEAAGIPHLGPGPAALPGPAPADWAQAPLEPAVRAVLVGFDEHFSYAKLCQALRYLLRGPDCLLVGTNRDNRLPLEGGSAIPGTGCLVKAVETAAEREALIVGKPSRYIFDCVASEFDIDPARTIMVGDRLDTDILMGNTCGLTTLLTLTGVSTLEEVRGHQESDCPARQGLVPDYYVDSIADLLPALED",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be designed to have high stability and activity towards H2O + sn-glycerol 3-phosphate = glycerol + phosphate.2. For general function, the protein need meet that However, their physiological relevance is unclear The designed protein sequence is ",
    "response#1": "ATYGTGSFILMNIGNNPIRSENLLTTIAWGLEKNKATYALEGSIFITGAAVQWFRDGLRAIDVSDEIEPLASNVEDNGGVYFVPAFVGLGAPYWDPYARGLIIGITRGTTKAHIARAILESMAYQTRDVIEVMQKESGISINSLKVDGGAAKDNLLMQFQADILGIKVIRPKVMETTSMGVAMLAGVL",
    "response#2": "ATYGTGSFILMNIGNNPIRSENLLTTIAWGLEKNKATYALEGSIFITGAAVQWFRDGLRAIDVSDEIEPLASNVEDNGGVYFVPAFVGLGAPYWDPYARGLIIGITRGTTKAHIARAILESMAYQTRDVIEVMQKESGISINSLKVDGGAAKDNLLMQFQADILGIKVIRPKVMETTSMGVAMLAGL",
    "response#3": "STYGTGCFALLNTGATPVASKNKLLTTIAYQLNGKRTYALEGSIFVAGSAVQWLRDGLGLIKQASETGPLADRSDATQSVYLVPAFVGMGAPYWNPNVRGALFGLTRNTGPAELAHAALESVCYQTFDLWAAMRADWPDAAAAHTVLRVDGGMTASDWTMQRLADLLDAPVDRPVIQETTALGAAYLAGLAVAEGIV"
  },
  {
    "reference": "MSETLATDATAPAEKKDFIRQIVREDLASGKHTVIRTRFPPEPNGYLHIGHAKAICLDFGLAAEFGGLCNLRLDDTNPAKEDPEFVVAIQDDVRWLGYDWAQLRHASDYFQVYYLAAQKLIRDGHAFVCDLSAEQVRQYRGTLTEPGRNSPFRERSVEENLDLFARMRAGEFPDGARTLRAKIDMASGNINLRDPALYRIKHVEHQNTGNAWPIYPMYDFAHSLGDAVEGITHSLCTLEFEDHRPLYDWCVDKVDLVGHPELLQPLLDKGLPREAAKPRQIEFSRLNINYTVMSKRKLTALVEEQLVDGWDDPRMYTLQGLRRRGYTPAAMRLFVDRVGISKQNSLIDFSVLEGCLREDLDAAAPRRMAVIDPLKLVLTNLPEGHTETLQFSNHPKDDSFGTREVPFARELWIEREDFAEVPPKGWKRLVPGGEIRLRGAGIARVDEVIKNADGEIVELRGWLDPESRPGMEGANRKVKGTIHWVSAAHAVEAEIRLYDRLFSVEKPDDESEGKTYRDYLNPESKRNVRGYVEPSAAMAAPEQAFQFERTGYFVADRRDHSEATPVFNRSVTLRDTWAK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The ATP, L-glutamine binding site should be located in a region of the protein that is accessible to the ligand.2. The protein should have a low Km value for ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-   glutaminyl-tRNA(Gln).3. A protein with ATP binding, glutamine-tRNA ligase activity functions is required to enable glutaminyl-tRNA aminoacylation, glutamyl-tRNA aminoacylation.4. The protein design must feature the 'HIGH' region, 'KMSKS' region motif. The designed protein sequence is ",
    "response#1": "EVIAQSLAAIRASQPTINAFTHVAEETAMQAAEAVDADRKAGKTLGPLAGLPVAIKDVLCTSDMPTTCSSKMLEGFVPPYDATVVARLKSAGAIVVGKTNMDEFAMGASTETSAMGVTGNPWDTTKTPGGSSGGAAAAVAAGAVPLSLGTDTGGSIRQPAAFCGITGLKPTYGRVSRYGLVAFASSLDQAGPMGWSVDDVAIGLQAMAGYDPRDSTSVNAEVPDFTPAMAAEDVRGMRIGVLREGLDQDGISPAVRDALATAESVFREQGAEIVEVELPHSKYWVPTYYVIAPCEASSNLSRFDGAHYGYRVADAEIAAADSGPLEAMYSLSRAGGFGSEVKRRIMVGTYALSEGYYDAYYNQALKVRRLIRNDYDAAFQQVDLMLGPVTPSPAFALNEKTDDPIAMYLCDLFTVGANLAGVPAISLPGGFDAVGLPVGVQLQAPVMEETRLLQVAHAFEQNTEFHKAPRAMAVIDPVRVVIESLPMGYEQDIAMPNHPNRPEMGTRQVAFSREIYIDRADFREEANKQYKRLVLGKEVRLRNAFVIKAEHVEKDPQGQITTIFCRV",
    "response#2": "LFTAKTGASNSKLPANWIIHELLGIINKLEIPLPKILQLLPASHFAEFLALIHDNELSKSSAKLLLFHIINDFKESHCSNLALPDFRLLIEQYNLNPLNEVEEHALTELCQYVLRELNDDALVADLVSGKKKNALKFLLGKGMRLSQGKVDPALLEGAFKRV",
    "response#3": "VIGLEVHAQIQTNSKLFSGAEVAFSSPINSCVSLFDASIPGTLPVLNRKCVELGVRTALALGCKVNSVSMFDRKHYFYADLPAGYQITQQRAPLARGGLLSFPVFVPGVSRKPYYKSARLHQLQLEQDSGKSLHDPVERKSLVDLNRAGVPLMELVFEPDLETGEEAAALVKELILILTRLGSCSCRMEEGALRVDANISVHKENTPLGTRTEVKNIGSVRAVAQAIEFEIERQIGILDGGGKIFNETRAWDAAGRQTIAMRDKEVVQDYRFMPEPNLPPLHVV"
  },
  {
    "reference": "MYDEKKVLLGMSLAELQQVVADLGMPKFTAGQIAKWLYQQHVATIDEMTNLSKANREKLNDSFQVGSMAPIDCQKSKDGTIKYLFPVASGHFVETVYIPDGDRATLCVSCQVGCKMNCLFCQTGKQGYEGSLSAGDILNQIYSLPERETLTNIVFMGQGEPMDNLDAVLRATEVLTSDYGYAWSPRRITVSSVGVKNKLKRFLDESECHVAISMHSPLPEQRQRLMPAEKQMSITEVVDLLKQYDFSHQRRCSFEYICFAGLNDTTTHAREIIKLLRGLDCRVNLIRFHEIPGVDLPGADEKRMEALRDYLTAHGVFTTIRASRGQDIFAACGLLSTAKKNEIEH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be able to bind S-adenosyl-L-methionine ligand in a variety of conditions2. The protein's S-methylcysteine intermediate, Proton acceptor must be carefully engineered to ensure that the amino acids involved in adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine are properly positioned for optimal catalysis.3. The ideal protein would be one that plays a critical role in rRNA base methylation through tRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding, rRNA (adenine-C2-)-methyltransferase activity, tRNA binding within cytoplasm.4. The stability of the protein-[4Fe-4S] cluster complex should be optimized to maximize enzymatic activity.5. The designed protein should contain one or more Radical SAM core domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MDKRPLIHGLSKSEIGQIVASLGQPSYRATQIWEWLYKRMAFEWNAMSNLPLALREHLDRELAICPGTLTARSADASGSEKLLLTLRDGECVEQVLIPAPGRKTVCVSSQVGCRFKCAFCASGQAGFRRNLEAGEIVAQTLWAALIWERRPSNVVFMGIGEPLDNYDNVLKAIRIINDSEGLGIGARKITISTSGIIPGMERLAGEGLQIELSVSLHAPTQELRSRLMPVARLYPLDRLIRACRSYFEKTKRLITFEYVLIRGTNDQASHARKLAELLSDFPCRVNLLLLSPVPEFKGTSSSPDAANAFIKTLSRAGINATLRAPRGLSVNAACGQLRSSPS",
    "response#2": "VGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGSGEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLHSPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKKCPNCCNGSRVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGSGEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLHSPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALAKLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKISTQVGCRMGCKFCASTLGGRVRDLTCGEILSQVIIGRKICGDRISNIVLMGSGEPLDNFDNVMKFLELVNADYGLNIGQRHITLSTCGIVPKIIELADKEMSITLAISLHAFSDEKRKEIMPIANKYSIIEILDACRYYINKTGRRITFEYSLVSGVNDGKEENTKLLSEINLLDLTLDELKQWMSENGENSFRAKQVLDWIYKGVFEFQQMNNIPKSSREKFNEVFTISIPRVLEKMVSKKDETRKYLLIYEDGNIIESVVMKYVYGYSICISTQIGCRMGCKFCASTIDGRVRDLTAGELIGEILAVQKDLGQRISNIVLMGSGEPLDNYENVIKFIKMVNEKYSLNIGQRHITLSTCGIVPKIYDLAREGLQITLAISLHAPNDIIRKQSMPVANSYSIDELIKSCQFYIDKTNRRVTFEYALIKGLNDSPSCAEELSKMLKGLLCHVNLIPINEVKEREYIKADKEIINEFKSILLKNGIEATLRREMGGDINAACGQLRRSY",
    "response#3": "MDKRPLIHGLSKSEIGQIVASLGQPSYRATQIWEWLYKRMAFEWNAMSNLPLALREHLDRELAICPGTLTARSADASGSEKLLLTLRDGECVEQVLIPAPGRKTVCVSSQVGCRFKCAFCASGQAGFRRNLEAGEIVAQTLWAALIWERRPSNVVFMGIGEPLDNYDNVLKAIRIINDSEGLGIGARKITISTSGIIPGMERLAGEGLQIELSVSLHAPTQELRSRLMPVARLYPLDRLIRACRSYFEKTKRLITFEYVLIRGTNDQASHARKLAELLSDFPCRVNLLLLSPVPEFKGTSSSPDAANAFIKTLSRAGINATLRAPRGLSVNAACGQLRSSL"
  },
  {
    "reference": "MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQAVEELRLTPVAARLGELLGKDVKKADEAFGPVAQEMVAAMNEGDVLVLENVRFYAGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIADYLPAVSGLLMEKELEVLGKALSNPERPFTAIIGGAKVKDKIGLIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCEDDKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTREIYADVIKNSKLVVWNGPMGVFEMTPFAEGTKAVGQALADAEGTYSVIGGGDSAAAVEKFGMADKMSHISTGGGASLEFMEGKELPGVVCLNDK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have ATP binding to facilitate glycolytic process.2. The protein should be able to bind ATP, substrate ligand in a variety of conditions3. A protein that is capable of catalyzing reactions within the glycolysis, pyruvate from D- glyceraldehyde 3-phosphate: step 2/5, Carbohydrate degradation pathway with high efficiency and specificity.4. The protein should have a fast turnover rate for (2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl   phosphate + ADP. The designed protein sequence is ",
    "response#1": "MSIILANPRGFCAGVDRAISIVERALELYGPPIYVRHEVVHNRFVVDGLRDRGAIFVEELSEVPDDTVIIYSAHGVSQAVRQEARSRNLTVFDATCPLVTKVHMEVSRLRRNGEESILIGHAGHPEVEGTMGQYASADGGMYLVESPEDVWSLKVKDESKLSFMTQTTLSVDDTSEVIDALKARFPNIVGPRKDDICYATQNRQDAVRDLAGKVDLVLVVGSKNSSNSNRLAELAQRVGTPAALLVSPEDLEPTWFDGVQTVGVTAGASAPEILVQQVIGRLQQFGGTEVVELLGREENIVFEVPKELRVEVKAIE",
    "response#2": "MPIPGVIPVTAAKG",
    "response#3": "MSGIQTHLNVADNSGAKEILL"
  },
  {
    "reference": "MTYRSIGSTAYPTIGVVLLGGIANPVTRTPLHTSAGIAYSDSCGSIRSETRIYADEATHIYFNGTESTDDNRSVRRVLDRYSSVFEEAFGTKTVSYSSQNFGILSGSSDAGAASIGAAILGLKPDLDPHDVENDLRAVSESAGRSLFGGLTITWSDGFHAYTEKILDPEAFSGYSIVAFAFDYQRNPSDVIHQNIVRSDLYPARKKHADEHAHMIKEYAKTNDIKGIFDLAQEDTEEYHSILRGVGVNVIRENMQKLISYLKLIRKDYWNAYIVTGGSNVYVAVESENADRLFSIENTFGSKKKMLRIVGGAWHRRPE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The desired protein should be able to perform (R)-mevalonate + ATP = (R)-3-phosphomevalonate + ADP + H(+) The designed protein sequence is ",
    "response#1": "SHPTGEVTVAFIDIISASKLWEYDPDGMCESTKMYNEIIRRVTKKYGGYESFISKDRNSGEGSFCLVFSDAIQAINSCEEMQLQLLNANWPKKILQHPAAAEEFDRTDQLIFRGLRVRMALHCGSVKISQDPMTRKYQYSGSTVNITGKITTLTHGGQIIVSENLYQKVNNDFTFITVGKIDIPDYPSKMTLYEC",
    "response#2": "AFPTIGIILLGGISDKKNRIPLHTTAGIAYTGINNDVYTETKLYVSKDEKCYIDGKEIDLNSDRSPSKVIDKFKHEILMRVNLDDENNLSIDSRNFNILSGSSDSGAAALGECIESIFEYNINIFTFENDLQRISESVGRSLYGGLTVNYR",
    "response#3": "EVTVAFIDIISASKLWEYDPDGMCESTKMYNEIIRRVTKKYGGYESFISKDRNSGEGSFCLVFSDAIQAINSCEEMQLQLLNANWPKKILQHPAAAEEFDRTDQLIFRGLRVRMALHCGSVKISQDPMTRKYQYSGSTVNITGKITTLTHGGQIIVSENLYQKVNNDFTFITVGKIDIPDYPSKMTLYES"
  },
  {
    "reference": "MINQSKLEYIITNIQSKKVLVIGDIMLDKYVFGSVRRISPEAPIPVINVENTKLLAGGAGNVIKNLNSLNIKTSFISIIGNDSSGKFLKTHFKKLKNIEYALLQDXNRKTTLKTRYIANGQQLFRTDEETISPLSSILKKKTFQYFKLFIKQSDIIIFSDYGKALFIEENFCQKLIKYATKEKKKVIVDPKGDYFEKYKGSYFITPNTKEASNATFIDPKDNKLVEKCGQYIIQQKWAQNVLLTRGENGLSIIQKKNTYHLKSNAEEVFDVTGAGDTVIALFSAALSITNNNIESAHFANLGASIAVKKLGTSSVSQDEILEVFRKFLKSKTLSSSSLLNNLNEWRANKYTIGFTNGCFDLIHAGHIDMLKKAAESCDKLIVAVNSDKSIKKLKGNKRPILNLEARKKILRSLEMVDFVISFEEDTPIKLIKQINPNILFKGADYKIKEIVGANFIKKNGGSVIRINLTKNHSTTKLVSMIKNS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7- phosphate at the C-1 position to selectively form D-glycero-beta-D- manno-heptose-1,7-bisphosphate.2. The protein should be optimized for activity in ADP-L-glycero-beta-D-manno-heptose from D- glycero-beta-D-manno-heptose 7-phosphate: step 3/4, Nucleotide-sugar biosynthesis, ADP-L-glycero-beta-D-manno- heptose biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The designed protein have Cytidyltransferase-like, Carbohydrate kinase PfkB domains with specific sequence motifs that are crucial for its activity.4. The designed protein must possess phosphotransferase activity, alcohol group as acceptor, ATP binding, heptose 7-phosphate kinase activity, heptose-1-phosphate adenylyltransferase activity to support ADP-L-glycero-beta-D-manno-heptose biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "MSRVLVVGDLMIDHYIWGSCDRISPEAPVQVVNIKNETKRLGGCGNVVSNLIALGAEVGVISVVGDDELGGEILKLLKDRGAKAELVIAQKGRKSSQKSRVMVTHQQVLRLDTESTSDIDCADEIVGKFQGILNSYDIVLLSDYGKGVLSDYLTKQIISITKNSAKMVLVDPKGKDYSKYKGATLLTPNKKEASEALGFVIDSDEKLELALKTMKMNYELEYSLITLSEDGIGLLAQKMVKFPALAKEVFDVTGAGDSVLATLGYCLASKMSIEDAIDNANLAAAVVVGKVGSALEEGNLVALVDDRDQALSRQTVLCIGDLMLDEFVYGEVSRISPEAPAPVIAVQRSETNVGGAGNVARNIASLGGRCIFVGLIGEDETGARLKTALAAESGIESRLVRDSARPTTRKVRFVSEHFSTHMLRADWEQAQPATGEIEQQLIDTILPELARADIVLLSDYAKGVLTARVIRNTIDAARKLGKRVIVDPKSLNLAIYRGATLLTPNRKEFAEATRSRADTPGAIAEAAEDAMRLADCEAMLVTQGERGMTLVVRDGETVHVPALPVKVRDVSGAGDTVAAALALSLAANADWETALRVASAAAAVAVGKTGTATATPAELRRVAEWRASGESIVFTNGCFDLLHVGHITLLEDCRRFGSKLVLGLNADSSVCRLKGPTRPIVGEKERARVMAALAAVDAVVLFEEDTPMELILSILPDVLVKGGDYTIDTVVGHQEVIAAGGRVEIVPTVAGFSTTNLVKK",
    "response#2": "MSRVLVVGDLMIDHYIWGSCDRISPEAPVQVVNIKNETKRLGGCGNVVSNLIALGAEVGVISVVGDDELGGEILKLLKDRGAKAELVIAQKGRKSSQKSRVMVTHQQVLRLDTESTSDIDCADEIVGKFQGILNSYDIVLLSDYGKGVLSDYLTKQIISITKNSAKMVLVDPKGKDYSKYKGATLLTPNKKEASEALGFVIDSDEKLELALKTMKMNYELEYSLITLSEDGIGLLAQKMVKFPALAKEVFDVTGAGDSVLATLGYCLASKMSIEDAIDNANLAAAVVVGKVGSALADGGGDLLTRDELLEVAEWRASGESIVFTNGCFDLLHVGHITLLEDCRRFGSKLVLGLNADSSVCRLKGPTRPIVGEKERARVMAALAAVDAVVLFEEDTPMELILSILPDVLVKGGDYTIDTVVGHQEVIAAGGRVEIVPTVAGFSTTNLVKKIGGDR",
    "response#3": "MSRVLVVGDLMIDHYIWGSCDRISPEAPVQVVNIKNETKRLGGCGNVVSNLIALGAEVGVISVVGDDELGGEILKLLKDRGAKAELVIAQKGRKSSQKSRVMVTHQQVLRLDTESTSDIDCADEIVGKFQGILNSYDIVLLSDYGKGVLSDYLTKQIISITKNSAKMVLVDPKGKDYSKYKGATLLTPNKKEASEALGFVIDSDEKLELALKTMKMNYELEYSLITLSEDGIGLLAQKMVKFPALAKEVFDVTGAGDSVLATLGYCLASKMSIEDAIDNANLAAAVVVGKVGSALEANAMTVEPVALPNFSQANILVIGDIMLDRYWHGDTSRISPESPVPVVNVAEVEERAGGAANVAINIASLGAKVTLCGITGDDVEATNLEALLGDASVHCRFEKRKNQTTITKLRVISRNQQLIRLDFEKDFALVHPLEIEKLEIEIENYDLVVLSDYNKGTLGRVADIIQSAKSKNVPVIVDPKGGSFEKYQGATLLTPNQSEFEAIVGKTNSDEDLLAKGKLLQERLDLTALLVTRSEKGMALIEKDKQPYLQATQAREISDVTGAGDTVIAALAASYACEKDWILASKIANQAAGIVVGKLGTATTNAQELQQLEKKKKIGQKE"
  },
  {
    "reference": "MKKPDIQQLKDIVNNSNQIVFFTGAGVSVASGIPDFRSMGGLYDEISKDGQSPEYLLSIDHLHDNKESFINFYHERLLIADKKPNIVHQWIAQLENQQKSLGVITQNIDGLHEDAGSHNIDELHGTLNRFYCINCYEEYSKSYVMTHHLKYCEKCGNVIRPDIVLYGEMLNQKTVFKALDKIQHADTLIVLGSSLVVQPAAGFVSEFKGDNLVIINRDATPYDHTASLVIHDDMTSVIEEIVNSNS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form..2. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for H2O + N(6)-acetyl-L-lysyl-[protein] + NAD(+) = 2''-O-acetyl-   ADP-D-ribose + L-lysyl-[protein] + nicotinamide.3. A protein that contributes to protein deacetylation by functioning in cytoplasm with zinc ion binding, NAD-dependent protein deacetylase activity, NAD+ binding, transferase activity.4. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation.5. The designed protein should be able to compete with endogenous proteins or inhibitors for nicotinamide, NAD(+) binding.6. The designed protein have Deacetylase sirtuin-type domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MTGKPLVAILSGAGVSTDSGIPDYRGPNGLWRRDPEAEKLVTYEYYMGDPEIRRRSWLMRRDSAALHAEPNAAHRAVADLERRGVPVRVLTQNVDGLHQLAGVSARKVLELHGTARDCVCTGCGARGPMADVLARIEAGEDDPPCLDCGGVLKTATVMFGERLDPVVLGEAAAISKACQVFVAVGTSLQVEPAAGLARVAVEHGARLVVVNAEPTPYDELADEVIREPIGSALPALLRGLL",
    "response#2": "MMTPEVMNKPVVVVLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFARNPQLVQEFYNARRRQLQQPEIQPNAAHLALARLEEAFGDRFLLVTQNIDNLHERAGNKNVVHMHGELLKVRCSQSGQVLEWTGDVTPGDKCHCCQFPAPLRPHVVWFGEMPLGMDRIYEALARADVFIAIGTSGHVYPAAGFVHEAKLQGAHTVELNLEPSQVGSEFEEKHYGLASQVVPEYVEKLLKP",
    "response#3": "MMTPEVMNKPVVVVLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFARNPQLVQEFYNARRRQLQQPEIQPNAAHLALARLEEAFGDRFLLVTQNIDNLHERAGNKNVVHMHGELLKVRCSQSGQVLEWTGDVTPGDKCHCCQFPAPLRPHVVWFGEMPLGMDRIYEALARADVFIAIGTSGHVYPAAGFVHEAKLQGAHTVELNLEPSQVGSEFEEKHYGLASQVVPEYVEKLLKK"
  },
  {
    "reference": "MKTKKSKSTLWFWLIILLAIIVTIIIIAVTVKGTTQVISDATFADWMSKSPQIDPNADKYWKNVIIYYGSNNTVVVKGSYFLESTGKYVNFVAYLTQKRFETIMADKPYPWPALVYQGSVGMALLVSLAPLLIYVLLFGGIIWFMMKSSSGAGAGAGNIFGMGKNRARAEKSDVKFANVAGIEEEKSELVELVDYLKFPAKYAEAGARAPKGVLMEGPPGTGKTLLAKAVAGEAGVSFFSIAGSEFEEMFVGVGASRVREMFNDAKKSAPAIIFIDEIDAVGRKRNNGMGSGGNEQTLNQLLVEMDGFGTNSGIIVMAATNRADVLDPALLRPGRFDRVIQVSLPDIKERKAILELHAKGKKIDGSVDWYRVAERTPGFSGAQLENVLNEAAILMVREKRDIITITEIDEAIDRVVGGPAKKSRAMTKQDKDIVSYHESGHALIGLKLDSASKVQKVTIIPRGNAGGYTIMTPKDETVFSSKKDLFATIAGYLGGRAAEEIMFGKENVTTGAHDDLDKATNIARRMVVQFGMSSLGMTKFLTMAEESYGKMEGTYSDETAARIDAEISKILEESYKIALKIIKENMETLELLAESLRVLETITAEQIEYINVNKKLPEEVLEQKNRMAKEDEKIKKGEIIDIKVEDLDID",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The Zn(2+) binding site should be stable and able to withstand changes in environmental conditions.2. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.3. The protein can be expressed in plasma membrane and perform ATP hydrolysis activity without triggering undesirable cellular responses.4. The designed protein should have at least Helical transmembrane domains to ensure proper localization.5. For general function, the protein need meet that Plays a role in the quality control of integral membrane proteins.6. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "LLVGPPGTGKTLLAKAVAGEAGVPFFSMSGSDFVEMFVGVGASRVRDAFRLAKEKAPCIIFIDEIDAVGRARSKNPAMGGNDERENTLNALLTEMDGFGTNSGVIVLAATNRADMLDKALLRAGRFDRQINVD",
    "response#2": "VLLSGPPGTGKTLLARAVAGEADVPFFSLAASEFVEAIVGVGASRVRDLFTQAKEAAPAIVFIDELDAIGRSRTSGVAGFSGGNDEREQTLNQILTEMDGFDSSTNVIVIGATNRPDVLDQALLRPGRFDRRVAV",
    "response#3": "VLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFENAKKTAPSIIFIDEIDAVGRQRGAGLGGGNDEREQTLNQLLVEMDGFQDDGNSVIVIAATNRSDVLDPALLRPGRFDRKVLVLFLE"
  },
  {
    "reference": "MFQGASSLSLDAKGRLSVPTRHRDVLSATAGGQLTITKHPHGCLMVFPRPEWEKFRERIAALPMSAQWWKRVFLGNAMDVEMDGTGRILVSPELRAATGIVRDTLLLGMGNHFELWDKATYEAKEAEATQGEMPDVFQDFAF",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein contains novel SpoVT-AbrB domains that confer a unique function or activity.2. The protein should have DNA binding, DNA-binding transcription factor activity and be active in nucleoid. The designed protein sequence is ",
    "response#1": "MFLGRFTHAIDDKGRLAIPARFREAFRGQGVLTRGIDRCLTLYPMDSWQPLAEKVSSLSISDPDARAFRL",
    "response#2": "MFLGRFTHAIDDKGRLAIPARFREAFRGQGVLTRGIDRCLTLYPMDSWQPLAEKVSSLSISDPDARAFRF",
    "response#3": "MFRGINAITIDTKGRLAIPTRYRSALGAEDKIPLVVTIDTEETCLLLYTAAQWQIIEDNLQKLPSFNAAARRIQF"
  },
  {
    "reference": "MARFRGSITKVSRRLGVALSPKAEKYLERRPFAPGQHGQSRKGKVSEYALQLREKQKMKYLYGILEKQFRNYYKKAVAQRGVTGDNLVKLLERRLDNVVFRSGFSASRAGARQLVSHGHLVVNGKKVNIPSFQVSPGDLIEFRQRSRNMGAVTDSLSKAPESRFPSWIQVDKANQKAVFLSVPEREDIQEPFNEQLVVELYSK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should contain one or more S4 RNA-binding domains that are essential for its function.2. The protein must exhibit the following characteristics: With S5 and S12 plays an important role in translational accuracy..3. A protein that can perform rRNA binding, structural constituent of ribosome in small ribosomal subunit to support translation. The designed protein sequence is ",
    "response#1": "RRLDNVVYRCGFSPSRAGARQLVTHGHMLVNGKKVNIPSFLVSPGDQIEFRQKSRNLDAVADSLR",
    "response#2": "LEPRKGNVPKREVLADPIHGSMVVAKLINSVMLDGKKGTAQNIVYGAFDIIKERAEENPVEVFEKAMNNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLRWLVKYTRARGEKGMIEKLAKEIMDAANNTGSSVKKKEDTHKMAEANF",
    "response#3": "PRKGNVPKREVLADPIHGSMVVAKLINSVMLDGKKGTAQNIVYGAFDIIKERAEENPVEVFEKAMNNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLRWLVKYTRARGEKGMIEKLAKEIMDAANNTGSSVKKKEDTHKMAEANF"
  },
  {
    "reference": "MRNSVLLCFWSIYCCFAAGSPTPFAPEGQLEDELHKSREVQAEAKPVRFNLRDSKDPEHEGCYLSPDHNQQLEDCGFNVTAKTFFIIHGWTMGGMLHNWLYKLVSALQTREKDANVVVVDWLPLAHQLYPDAVNNSRVVGHSVARMLDWLQEKDGFSLGNVHLIGYSLGAHVAGYAGNFVKGTVGRITGLDPAGPMFEGVDIHRRLSPDDADFVDVLHTYTRSFGLSIGIQMPVGHIDIYPNGGDFQPGCGFNDVLGSLAFGTITEVLKCEHERAIDLFVDSLVNQDKPSFAFQCTDSKRFKKGICLSCRKNRCNSIGYNTRKMRSKRNTKMYLKTRANMPFRVYHYQMKIHVFSYKNKGKIEPTFYVTLYGTNADSQVLPLEIVEHIGLNATNTFLVYTEEDLGDLLKVRLTWEKTSQSWYHLWKELRSLFTQPSGSAQELHIRRIRVKSGETQQKLTFCAEDLENSSISPGQELWFHKCRDGWRMKNETRATMELA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must contain a signal peptide for proper functionality.2. The designed protein should have lipoprotein lipase activity, heparin binding within early endosome, Golgi apparatus to regulate lipid catabolic process.3. The protein contains novel PLAT domains that confer a unique function or activity.4. The Ca(2+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "DWMVDFSDIESKFALRTSDEPEDDLCYIVPGQRETIKECNFNPDTKTFIVIHGWTVTGMYESWVPKLVTALYEREPTANVIVVDWLIRAQQHYPTSAAYTKLVGRDVAKFVNWLQAELEYPWEKLHILGYSLGAHVAGIAGLLMRNKVNRITGLDPAGPTFEYADAQSTLSPDDALFVDVLHTNTRGSPDRSIGIQRPVGHVDIYPNGGTFQPGCDLQNTMMMVATTGLRNMDQIVKCSHERSIHLFIDSLINMEQQSMAYRCSSKETFNKGVCLSCRKNRCNKVGYDVTKVRSRRNSKMYMKTRDMMPE",
    "response#2": "TKTKFLLFQDEVSRDCQLRLHHPESLQECSFNSSWPLVMIVHGWSVDGLLENWIWQMAAALKSELAQPVNVVLVNWLTLAHNHYTTAVHNTRLVGQEIAALLRWLEEAVAFSRSKVHLIGYSLGAHVSGFAGRYIGGKHKIGRITGLDAAGPLFEKASPRDRLSPDDADFVDAIHTFTQEYMGLRVGIEQPIGHYDFYPNGGSFQPGCHILEVYKHIALHGLKAIPQTLKCAHERSVHLFIDSLRHKEQQSVGYQCTDMDRFNQGLCLSCKKGQCNRLGYYIRQDRPRRSKKLFLITRR",
    "response#3": "QDVETKETLQEMKSRFLLFEEADKGCQIRPYHPNTLQECGFNASRPLVMIIHGWSVDGVLEAWIWQLVAALKSGLSQPVNVGLVDWVTLAHQHYVIAVRNTRLVGKEVAALLQWLEESVQFSRSNVHLIGYSLGAHVSGFAGSYIGGTHKIGRITGLDAAGPLFEGAPPSDRLSPDDASFVDAIHTFTQEHMGLSVGIKRPVAHYDFYPNGGSSQPGCHFLELYKHFAKHGLNAITQTIKCAHERSVHLFIDSLLHGSLQSTAYLCSDMGSFSQGLCLSCQKGRCNTLGYHVRQKPQSKSRSLYLVTRAQSPV"
  },
  {
    "reference": "MMRIAVVGASGRMGLCLIKAAALAKNAELAVAVSRPESLAIGRDAGELAGISVVGVKVGDDLASVVDQFDVLIDFTRPDASMAFIETCRQAGKKLVIGTTGYSDAQKALISDAAKDVAIVMAPNMSVGVNLSLKLLEMTAKVMGDYTDIEVIEAHHRHKVDAPSGTALRMGEVIADTLGRDLKDCAIYGREGNTGERDRKTIGFSTIRAGDIVGEHTVMFADDGERLEITHKATSRMTFANGAVRAAVWLADKDSGLYDMQDVLGLS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should take into account the physiological conditions of the L-lysine biosynthesis via DAP pathway, Amino-acid biosynthesis pathway.2. The designed protein should be able to compete with endogenous proteins or inhibitors for (S)-2,3,4,5-tetrahydrodipicolinate, NAD(+), NADP(+) binding.3. Ensure the presence of a signal peptide within the protein.4. The protein design should aim to mimic or enhance the function of natural proteins involved in diaminopimelate biosynthetic process, lysine biosynthetic process via diaminopimelate, potentially with improved properties such as stability, specificity or selectivity.5. The Proton donor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.6. The designed protein have Dihydrodipicolinate reductase N-terminal domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MHVGVIGCSGRTGKVIVSALEQSSEYTLGPGFSRSSALTLFQVIAHNDVLVDFSHPLLTKEVVAHLLISPKPLIIGTTGFPGKCKEAHDSLEELTHIVPVVVCPNTKLVMAAEKYFQSKGLGAYIHKRLVMLLSQLCNPQFDIRIRETHHRYKKDSLSGTAQDLLDTIQQVKQEDWGEEYEVGQRDSSKKTIEVQSSRVGDIPGEHEVAFISSGEQILVRHTVFSRNVFGRGILSILDWLKTLNPQPGLYSLGDTGAVLMMQFAKTAAKYLPDVEIIEKHHDQKLDAPSGSAVKTADMIKETRESKQQGHPDEREIMNGARGAETDGIRIHSVRLPGLVAHQEVIYGGPGQTLTIKHDSYNRQSFMDGVKLSVNEVINLNKLVYGLEKILS",
    "response#2": "MRVAVVGAKGKVGTTMVEAVRAAPDLELSAAVDADDPLSLLTDGGTEVVIDFTHPDVVMDNLKFLIENGIHAVVGTTGFTAQRLDTVAGWLAERPGVGVLIAPNFAIGAVLMMKFAKMAATYFPDVDIIEKHHDQKLDAPSGTAIKTAELISESRKVKTQGHPKEEETIPGARGADLDGIKLHSVRLPGLVAHQEVIFGSPGQTLTIKHDSMDRSSFMDGVKLCVNKVMEVKGLIYGLDTIL",
    "response#3": "MRVGVLGARGKVGTTMVEAVEAADDLTFAAGVDADDPLSRLVDAQTEVVIDFTHPEVVMDNLRFLVENGIHAVVGTTGFTDERLAQVRQWLDAKPGAAVLIAPNFAIGAVLMMQFAKTAAKYLPDVEIIEKHHDQKLDAPSGSAVKTADMIKETRESKQQGHPDEREIMNGARGAETDGIRIHSVRLPGLVAHQEVIYGGPGQTLTIKHDSYNRQSFMDGVKLSVNEVINLNKLVYGLEKILGLK"
  },
  {
    "reference": "MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDNPEEEYGYEDLKESSNSLLNHQLSEIDQARLYSCLDHMREVLGDAVPDDILTEAILKHKFDVQKALSVVLEQDGVQTLKEKSERAVCAGQPSKVISRSSQSESEIVPKVAKMTVSGKKQTMGFEVPGLPSEENGHNVRAPYKGPPGDDVSIASPNVPETGTPKSTAHPPSLQTSEELGCTPTPLRKSGKLRQQIDVKAGLEKRQGGKQLLNLVVIGHVDAGKSTLMGHMLYLLGNVNKRTMHKYEQESKKAGKASFAYAWVLDETGEERERGVTMDVGMTKFETTTKVVTLMDAPGHKDFIPNMITGAAQADVAVLVVDASRGEFEAGFETGGQTREHGLLVRSLGVTQLAVAVNKMDQVNWQQERFQEITGKLGHFLKQAGFKESDVAFIPTSGLSGENLTSRSQSSDLTKWYKGLCLLEQIDSFKPPQRSIDKPFRLCVSDVFKDQGSGFCVTGKIEAGYVQTGDRLLAMPPNETCTAKGITLHDEPVDWAAAGDHVSLTLVGMDIIKINVGCIFCGPKEPIKACTRFRARILIFNIEVPITKGFPVLLHYQTVSEPAVIKRLISVLNKSTGEVTKKKPKLLTKGQNALVELQTQRPVALELYKDFKELGRFMLRYGGSTVAAGVVTEIKE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.2. A protein with translation elongation factor activity is needed to enhance translation in a specific context.3. The protein must exhibit the following characteristics: Cotranslational quality control factor involved in the No-Go Decay (NGD) pathway.4. The designed protein should be able to compete with endogenous proteins or inhibitors for GTP binding. The designed protein sequence is ",
    "response#1": "LRTPIVAVLGHVDHGKTSLLDTIRGSAVSEGEAGAITQHIGATDIPLDTISEMAGELIDPTDFDLPGLLFIDTPGHHSFSTLRARGGALADIAVLVVDVNDGFQPQTEEAIDILRRTGTPFVVAANKVDTTPGWNPQDGQPIQRSLEAQSERAESMLNENLYEIIGQLSDAGFSADLYWRVQDFQKNIGVVPLSAITGEGVPDLLTVLMGLSQRFMA",
    "response#2": "PPVVTVMGHVDHGKTRLLDTIRKANVREAEQVTVEHDGVERPITFIDTPGHEAFTAMRARGAKATDIAILVVAADDGVMPQTVEAINHAQAADVPIVVAVNKIDVEGADPQKIRGQLTEYGLVPEEFGGDTMFVDISAKQGTNIDQLLEAFK",
    "response#3": "RNFSIVAHIDHGKSTLADRLIQMTGGLETREMKEQVLDSMDIERERGITIKAQTVRLHYKAKNGETYILNLIDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVYQAIDNSHELVVVLNKVDLPAAEPERVKEQIEDVIGIDTSEAVEISAKTGFGVPDVAEA"
  },
  {
    "reference": "MRNFLDKIGEEALVVEDEVSTSFEAASILREHPRDLVILKNLKESDIPVISGLCNTREKIALSLNCRVHEITHRIVEAMENPTPISSVGGLDGYRSGRADLSELPILRHYRRDGGPYITAGVIFARDPDTGVRNASIHRMMVIGDDRLAVRIVPRHLYTYLQKAEERGEDLEIAIAIGMDPATLLATTTSIPIDADEMEVANTFHEGELELVRCEGVDMEVPPAEIILEGRILCGVREREGPFVDLTDTYDVVRDEPVISLERMHIRKDAMYHAILPAGFEHRLLQGLPQEPRIYRAVKNTVPTVRNVVLTEGGCCWLHAAVSIKKQTEGDGKNVIMAALAAHPSLKHVVVVDEDIDVLDPEEIEYAIATRVKGDDDILIVPGARGSSLDPAALPDGTTTKVGVDATAPLASAEKFQRVSRSE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must be able to carboxy-lyase activity. The designed protein sequence is ",
    "response#1": "MLMGLPKEPQIYASVKKVVPKVHGVRLTEGGCMWLHAVVSITKQHEGDGKNAILAAFAGHPSLKRVVVVDEDVNIYDDREVEWAIATRFQPDRDLVIIPNARGSSLDPSGKDGLTAKWGIDV",
    "response#2": "FLKNREKVNLLELPIPKYYPKDGGPYLTSAMVIAKKEFVNVSFHRMMVLDEERAVIRLVPRHLYSMWKDSVEHGEELEVRIVLGNPVHLLLAGATSVAYGVSELEIASAISLKAFGRPLEVINLDGIPTPVDSEFVFKAKITDEVADEGPFVDITGTYDIVRKQPIVIFEEMYHVDDPIFHALLPGQ",
    "response#3": "FLKNREKVNLLELPIPKYYPKDGGPYLTSAMVIAKKEFVNVSFHRMMVLDEERAVIRLVPRHLYSMWKDSVEHGEELEVRIVLGNPVHLLLAGATSVAYGVSELEIASAISLKAFGRPLEVINLDGIPTPVDSEFVFKAKITDEVADEGPFVDITGTYDIVRKQPIVIFEEMYHVDDPIFHALLPGR"
  },
  {
    "reference": "MLTSKRILLIIGGGIAAFKSLDLIRRLRERGAEVTPVLTRAGTEFVTPLSVSALAGRRVFSDLFDLTDEAEMGHIELSRSADLIVVAPATADLMAKMAHGHADDLASTLLLATDTPVLLAPAMNVRMWQHAATQRNIETLIGDGIGFVGPNEGDMACGEHGPGRMAEVPEILAAVEAALADGPLRGRRVLVTSGPTHEPIDPVRYIANRSSGAQGTAIARALAALGAEVVFVTGPATVPPPEGVEVIRVETARHMLAAVEAALPADAAIFAAAVADWRMAQPTDRKIKKTEGGLPVLEFAENPDILATVSQIAEGRPSLVVGFAAETHDVVEHATAKRARKGCDWIVANDVSAGTGIMGGTENAVTLITDEGAESWPRMGKAQVARHLAERIAQALA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must have high specificity towards phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity.2. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..3. The Proton donor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.4. Ideal protein should have a binding affinity for key enzymes in the Cofactor biosynthesis, coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MNIVLGVGGGIAAYKSALLLRLFTEAGHKVTVIPTEASTKFVGVATWEALSGRPATNDVFDAVDQVNHVRIGHEADLIVVAPATADLLAKAAGGQANDLLTTTLLMARGPVLFAPAMHTEMWRHAATQANVATLRSRGIHVLDPAVGRLTGPDTGPGRLPEPEAIFAAAEVAAVLAARPGVAEDRRVVITGGGTREKIDEVRYISNFSSGKMAKAIALSLYLKGADVCYITTKGNDSLPKSIYTIEVDDAQEMLEYTQDALRIAKKGKLSKASLTTADAIKLIQKRPFLFMVAAVADFTPKFPQQGKLKKSTLGENWQIELTQTSDILSTLAKDREGVTTIAFKAEMDTQEGLNNAKELLTKKEVDAVCYNLLEDAKSFGGDQNSITFLFANGAVEELPVASKDELAAVVLDRVVKLLGG",
    "response#2": "MNIVLGVGGGIAAYKSALLLRLFTEAGHKVTVIPTEASTKFVGVATWEALSGRPATNDVFDAVDQVNHVRIGHEADLIVVAPATADLLAKAAGGQANDLLTTTLLMARGPVLFAPAMHTEMWRHAATQANVATLRSRGIHVLDPAVGRLTGPDTGPGRLPEPEAIFAAAEAARLLLERPDLDGRRVLVTAGPTREPLDAVRVVTNRSSGQMGYALAAEAWARGADVVLLSGPVALPTPHGVARRDVETTADLGAALQDELPRADVLLMAAAPADYRAAEVARGKRPRQEGALSLDLAPTDDLLQATRASRKPGALMVGFALEAGDAVERARAKLVRKDLDLIVVNDALEPGAGPEVPTNRVTLLSPDGEVEELPLMSKDAVADVILDRVLTLMS",
    "response#3": "MNIVLGVGGGIAAYKSALLLRLFTEAGHKVTVIPTEASTKFVGVATWEALSGRPATNDVFDAVDQVNHVRIGHEADLIVVAPATADLLAKAAGGQANDLLTTTLLMARGPVLFAPAMHTEMWRHAATQANVATLRSRGIHVLDPAVGRLTGPDTGPGRLPEPEAIFAAADLLVAALEAAGAESLDGRRVLVTAGPTREPLDAVRVVTNRSSGQMGYALAAEAWARGADVVLLSGPVALPTPHGVARRDVETTADLGAALQDELPRADVLLMAAAPADYRAAEVARGKRPRQEGALSLDLAPTDDLLQATRASRKPGALMVGFALEAGDAVERARAKLVRKDLDLIVVNDALEPGAGPEVPTNRVTLLFADGTREELPLMSKGQVAQAVIERVERLLRQ"
  },
  {
    "reference": "MHTEKVNIKSYSRKDLQHVVAELGEPGYRANQIHRWLFNEQVENFESMTNLGAKLRNRLSNRYVIQSCSIVKKQLDPEKKNGMGTSKFLVKLHDEQTVETVLIPAEDRNTVCVSSQVGCPLRCTFCATGYMGFTRNLNAAEMAEQVYLVNNLLTATPTNHVTNVVFMGMGEPLLNTSNVIEAIETLSDKDYSFSLPQRKITISTVGLIPQIDAIARSGIKTKLAVSLHAANQQKRASLMPVAKEHTLDGLHNALERYVDLTGKPVTLVYMLLEGINDTMEDAKSLVAFCSGFLCKINLIDYNSIVNMKFKPVETEWRDRFIRILVGAGLNVTVRKSYGTSINAACGQLALKEKTLPADRFIK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein with 4 iron, 4 sulfur cluster binding, tRNA (adenine-C2-)-methyltransferase activity, rRNA (adenine-C2-)-methyltransferase activity is needed to enhance rRNA base methylation in a specific context.2. The protein should be able to bind [4Fe-4S] cluster, S-adenosyl-L-methionine ligand in a variety of conditions3. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity.4. The protein must be engineered to have a high affinity for [4Fe-4S] cluster in order to increase its catalytic activity.5. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs..6. The protein have enhanced adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "QEYGMSVCVTTQVGCNIGCTFCASGLLKKQRDLTAGEIVAQIMLVQRYFDERGKDERVSHIVVMGIGEPFDNYNNLMDFLHIINDAKGLAIGARHITVSTSGLVPKIKEFAKNGLQVNLAISLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLKGVNDRPEHAQQLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSKREDVMKFYDILKKNGVNCVIRKEHGTETNG",
    "response#2": "QEYGMSVCVTTQVGCNIGCTFCASGLLKKQRDLTAGEIVAQIMLVQRYFDERGKDERVSHIVVMGIGEPFDNYNNLMDFLHIINDAKGLAIGARHITVSTSGLVPKIKEFAKNGLQVNLAISLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLKGVNDRPEHAQQLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSKREDVMKFYDILKKNGVNCVIRKEHGTEA",
    "response#3": "QEYGMSVCVTTQVGCNIGCTFCASGLLKKQRDLTAGEIVAQIMLVQRYFDERGKDERVSHIVVMGIGEPFDNYNNLMDFLHIINDAKGLAIGARHITVSTSGLVPKIKEFAKNGLQVNLAISLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLKGVNDRPEHAQQLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSKREDVMKFYDILKKNGVNCVIRKEHGTTIN"
  },
  {
    "reference": "MDSGLGSSYPEERSGPPEIRPEEINFEELIGTGSFGKVYKGRCRQKAVAVKLLHKQNFDAATLSAFRKEVHLMSKIYHPNICLFMGACTIPGRCVIVTELVPKGNLETLLHDQKIQLPLYLRMRMARDAALGINWLHESNPVFVHRDIKSSNLLVDENMRVKICDFGLSALKQKHKMLKDQSSAKGTPLYMAPEVMMFKEFNESSDVYSFGIVLWEILTRKEPFSHHRELEKFREAVCVKHERPPIPNDCLDSLRRLIEKCWDKEPISRPSFKEIISALDHVIIDAAISDLNGRDFWKKSFLTEQEVPWEAFIDALCNWSKAPARNQCDKTSIDYLNIKCLKAVLAESPKSEGTGVETVVNIEKFGKILEFFGPVPEPAPGHSIMDTVREILSQKWFHGDLDTTEAASRLNGQPIGSFLIRFSSTNAGCFTISQVVDGGSIKHQRVSRQGVKFNYQNVIYNSLIEIVSKNGGGANGDVKLDSQLGVPNYKFMSLFCVLNKESEVYQ",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.2. The designed protein should have a binding site that is specific to the target ATP ligand.3. The designed protein must be optimized for ATP binding. The designed protein sequence is ",
    "response#1": "MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAV",
    "response#2": "FNPDNQLGEGGFGRVYKGQIETPEQVVAVKQLDRNGYQGNREFLVEVMMLSLLHHQNLVNLVGYCADGDQRILVYEYMQNGSLEDHLLELARNKKKPLDWDTRMKVAAGAARGLEYLHETADPPVIYRDFKASNILLDEEFNPKLSDFGLAKVGPTGGETHVSTRVMGTYGYCAPEYALTGQLTVKSDVYSFGVVFLEMITGRRVIDTTKPTEEQNLVTWASPLFKDRRKFTLMADPLLEGKYPIKGLYQALAVAAMCLQEEAATRPMMSDVVTALEYL",
    "response#3": "MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAG"
  },
  {
    "reference": "MTKYVFVTGGVLSSVGKGIVAASIGKLLKARGYSVTAIKIDPYLNVDAGTMNPLMHGEVFVTEDGGETDLDLGHYERFLDINLSRNNNITTGQIYSTVIERERKGEYLGQCVQIIPHITDEIKRRIRLVARETQVDVVVVEIGGTVGDIEGLPFLEAIRQMRIEEGFENTLFVHVALVPILEVTGEQKTKPIQHSVQELRRIGIQPDIIIARCKVPLEMEAKWKIALYGNVPVDSVFTSYTVETIYELPLILEKQGLGSKVTSKLKLENRSPDLTDLEWFVSRLKKLSKKATIGMVGKYTKLKDSYLSINEALIHAGAFANVKPVLKWIEATDVEKGKLTPKEALEGVNGIIVLPGFGRRGAEGKISIIKYARENNIPILGICFGMQLMVVEFARNVVGLEKANTTEIDPQTPHPVVDLMEEQLKIDRLGGTMRLGAYRIDLEKNTLVYTLYGKRSIYERHRHRYEVNLKYVNKLTEAGIVVSGRSYNGGVEFIELPTKQHKFYLGTQAHPEFKSRPLKPSPPYLGFLKAIVSS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should interact with enzymes and substrates within the CTP biosynthesis via de novo pathway pathway to enhance or regulate its activity.2. The protein must have high specificity towards metal ion binding.3. The designed protein should contain one or more CTP synthase N-terminal domains that are essential for its function.4. The protein should have a high affinity for L-glutamine, Mg(2+), ATP, CTP. The designed protein sequence is ",
    "response#1": "SDSYLSVIKALQHSAFAVDRKLVIDWIESTDLDPHNVTDKHDEAWGLLRAADGILVPGGFGNRGVEGKIAAANYARINNVPYLGVCLGLQIATIEFCRNVLGLENANSTEFDENTPNPAVIFMPEISKTHMGGTMRLGTKPTPFLVDDCKIRRLYGGVDHVDERHRHRYEVNPDLIERIEAEGLVYVGKDETGQRCEIMELENHPYYVGTQYHPEFKSRPNRPSPPFLGLLG",
    "response#2": "VEIALVGKYIDLPDAYLSVTEALRAGGFHHDARVAIRWVASDTCQTPEGAQEALGGVDAVLVPGGFGVRGIEGKLGALRWAREHKVPTLGICLGLQCMVIEAARNLAGIEGASSSEFDPETPAPVIATMAEQKAFVEGAGNLGGTMRLGAYPAKLAKGSVVAGAYGATKASERHRHRYEVNNAYRARLEEAGLVFSGTSPDGTLVEFVELPRDVHPYYVSTQAHPEFKSRPHRAHPLFAGLIAGITIGLVGKYVALPDAYISVVEALRHAGLYHGVAVDVRWINSEELEEQPAEKLLSDVDGVVVPGGFGDRGIEGKINAINYTRINKVPFLGLCLGMQLAVVEYARNVLHWQGANSTEFNPDTPYPVIDLLPEQKELDKMGGTMRLGKYPCRLMEDTLASNSYGEEVIYERHRHRYELNNKFRDELADAGVVFSGTLPDRYLVEIIELSDHPWFLATQFHPEFKSRPYRPHPLFREFIRIAIVGKYTEIEDAYLSIREALKHAGVDAGCIVDIKWIEAEELENLENPEELLGDVGGILVPGGFGIRGAEGKIKAIKFAREHKIPFLGICFGFQLAVVEFARHRLGWEGASSTEITDTPYPVIEILPEQLDVEDLGGTMRLGDSEITVLKGTRAYAIYGKEQIVERHRHRYEVNPEYIEAIEEGGMRFSGKSGNRMEILEIADHPFFIGTQFHPEFSSRPGKPAPVFRAFITIGLVGKYVALPDAYISVVEALRHAGLYHGVAVDVRWINSEELEEQPAEKLLSDVDGVVVPGGFGDRGIEGKINAINYTRINKVPFLGLCLGMQLAVVEYARNVLHWQGANSTEFNPDTPYPVIDLLPEQKELDKMGGTMRLGKYPCRLMEDTLASNSYGEEVIYERHRHRYELNNKFRDELADAGVVFSGTLPDRYLVEIIELSDHPWFLATQFHPEFKSRPYRPHPLFREF",
    "response#3": "LPDAYLSVVEALRAGGFEHSARVTLRWVASDDCVTPAGAARQLHDVDAVCVPGGFGVRGIEGKIGALRYAREEKIPTLGLCLGLQCMVIEHARNVAGLPGASSSEFDVDPEHPVIATMQEQKAFVEGSGDLGGTMRLGLYPATLREGSLVARTYAQTLVQERHRHRYEVNNAYREVLESSGLIFSGTSPDGTLVEFVELAGDVHPYYVATQAHPELRSRPTRPHPLFAGLVR"
  },
  {
    "reference": "MRVRGIPRNWQQWWIWGILGFWILMICNVVGNLWVTVYYGVPVWKEAKTTLFCASDAKAYEREVHNVWATHACVPTDPNPQEIVLENVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLTCKDYNKNITYDNNSTIGEIKNCSFNTTTELRDRKKNVYALFYKIDIVPLNDNNSNSNNSMYRLINCNTSAITQACPKVSFDPIPIHYCAPAGYAILKCNDKTFNGTGPCQNVSTVQCPHGIKPVVSTQLLLNGSLAEEEIIIRSENIEDNVKTIIVHLNESIEIVCIRPNNNTRKSIRIGPGQTFYATGDIIGNIREAHCNITREEWNKTLQGVEEKLKEHFPNKKITFKPSSGGDLEVTTHSFNCGGEFFYCNTTHLFNGNGTESDINITLPCRIKQIINMWQEVGRAMYAPPIAGNITCKSKITGLLLTRDGGKTNQNESKIFRPGGGDMRNNWRSELYKYKVVEIKPLGLAPTGAKRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASTTLTAQARQVLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKSQADIWDNMTWMQWDREISNYTDTIYRLLEDSQIQQEKNEKDLLALDSWKNLWSWFDITNWLWYIRIFIIIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTLTPNPRGPDRLGGIEEEGGEQDRDRSIRLVNGFLALAWDDLRSLCLFSYHRLRDCILIAARAV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should contain at least one Di-leucine internalization motif motif to achieve its intended function.2. The protein must exhibit the following characteristics: Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes.3. The protein should have a Helical domain that is long enough to span the entire membrane.4. The designed protein should be involved in actin filament reorganization, clathrin-dependent endocytosis of virus by host cell, positive regulation of plasma membrane raft polarization, virion attachment to host cell, mitigation of host antiviral defense response by localizing to viral envelope, virion membrane, plasma membrane, host cell plasma membrane and exhibiting structural molecule activity.5. The designed protein have Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "PVKKCEFNVTTVLKDKKEKKQALFYVSDLVRVNNSKTSSSNTTYTLINCNSTTIKQACPKVSFEPIPIHYCAPAGFAIFKCNSIEFNGTGTCHNITVVTCTHGIKPTVSTQLILNGTISEGKIRMIAKNISVGSRNIIVTLNTTINMTCQRPGHQEIQEIKTGPLAWYSMYLKENTTESRKAYCEYNVNNWEEALRQTAERYLELVNNTGVISMIFNHSAGGDPEVSQLHFNCHGEFFYCDTTQMFNYTFNCTDTRCDNQSTTSSNNIPCRLRQVVRSWMRGGSGLYAPPIRGNLTCGSNITGMILQMDQPWNGSQSNTNRSSTFRPIGGDMKDIWRTELLRYKVVRIKPFSVAPTKITRKVARVRLTSGFEITAYCKKDLRKGRSTFKGQKD",
    "response#2": "MRVMGILRNCQQWWIWGILGFWMLMICNVKGNLWVTVYYGVPVWKEATTTLFCASDAKAYKTEVHNIWATHACVPTDPDPQEVDLTNVTEEFNMGKNNMVDQMHEDIISLWDQSLKPCVKLTPLCVTLYCTDARNSTNDMTMKEIKNCSFNITTTMRDKVQKQYAIFNSLDVVQIEEDNNTSGTNYSSYRLRSCNTSTVTQACPKVSFEPIPIHYCAPAGFAILKCKDKQFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSENFTDNAKNIIVQLNEPVEINCTRPNNNTRKSVHLGPGRAFYTTGDIIGDIRRAYCTINGTKWNNTLHQIVKKLREQFGNNKTIVFNQSSGGDPEIVMHSFNCGGEFFYCNSTQLFNSTWNVTEGLYNTGENGTITLPCRIKQIINMWQGVGRAIYAPPIAGRITCSSNITGLILTRDGGRDGGNVTSQDETFRPGGGNMKDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLKAIEAQQHLLQLTVWGIKQLQARVLAIERYLKDQQLLGLWGCSGKLICTTTVPWNSSWSNKTFKYIWENMTWMEWEREIDNYTDTIYRLLEDSQSQQEKNEKDLLALDSWNSLWSWFSISNWLWYIKIFIMIVGGLIGLRIVMAVLSIVHRVRQGYSPLSFQTLTPNPRGPDRLGRI",
    "response#3": "MGAAATALTVRTHTLIKGIVQQQDNLLRAIQAQQHLLRLSVWGIRQLRARLLALETLIQNQQLLNSWGCQGKLVCYTSVKWNRTEWKGNESIWGNLTWQQWDQEIDNVSATIFEEILKAQVQQEANEKKLLELDEWASIWNWLDITKWLWYIKTAIIIVGALIGIRIVMVILNLVRNIRQGYQPLSFQIPL"
  },
  {
    "reference": "MDFFSEYGDSSRYKIQEIVGKGSYGVVCSAIDQHTGDKVAIKKIHNIFEHLSDAARILREIKLLRLLRHPDIVEIKHIMLPPSRRDFKDIYVVFELMDTDLHQVIKANDDLTKEHHQFFLYQMLRALKYIHTANVYHRDLKPKNILANANCKLKICDFGLARVAFNDTPTTVFWTDYVATRWYRAPELCGSFFSKYSPAIDTWSIGCIFAEILTGKPLFPGKNVVHQLDLMTDLLGTPSMDAISRIRNDKARRYLSSMRRKQPVPFSEKFPNVDPLALKLLQRLLAFDPKDRPTAEEALADPYFKGLAKVEREPSCQPISKMEFEFERRKVTKDDIKELIFREILEYHPQLLKDYMNGSENTSFLYPSAVDNFRRQFAILEENGGKSGALDRKHVSLPRATTVHSTSIPPNEGLDATSQVTQRIPTARPGRTVGPVLPFENPGAADPHSARRVVRNPMVPPAAANKSGYSYNLKSDYSDRQHQEELEKDRVQYRPAQHLMDAKVAPDTAPDIRSSQYYFTRSAPRTDLTDRAALQGSMLYGIAPFNGIAAVAGGYSKVGAVQYGVSRMY",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-   threonyl-[protein].2. A protein that can perform MAP kinase activity, protein serine/threonine kinase activity, ATP binding, protein serine kinase activity in cytoplasm, nucleus to support intracellular signal transduction, protein phosphorylation.3. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.4. The presence of Protein kinase domains is critical for the interaction of the designed protein with its intended targets.5. The protein design must feature the TXY motif. The designed protein sequence is ",
    "response#1": "GKQLGSGGFGLVYQVSCKSKEIDCVAKIELKESGGLFCEINFYNRVMKNKTSLDTWMKEQKIDYIGIPSFHGFGITIYKNVEYRFAIIQRLGRDLENILSEKEKFNITVIKKLAIKILDILKFIHSKEFSHGDIKAGNILFGKDDDKVYLVDYGLATKYSSNGKHKEYTINPKNRHNGTMAFTSIDAHKGVTVSRRGDLESLGFCMLKWYSGKLPWEKYEKEPENVQGMKYNIGRLLGTGNFAKVYHGTEISTGDDVAIKVIKKDHVFKRRGMMEQIEREIAVMRLLRHPNVVELREVMATKKKIFFVMEYVNGGELFEMIDRDGKLPEDLARKYFQQLISAVDFCHSRGVFHRDIKPENLLLDGEGDLKVTDFGLSALMMPEGLGGRRGSSDDLLHTRCGTPAYVAPEVLRNKGYDGAMADIWSCGIVLYALLAGFLPFIDENVMTLYTKIFKAECEFPPWFSLESKELLSRLLVPDPEQRISMSEIKMIPWL",
    "response#2": "LPRKLFYTWIYQICLGVQSIHKNHLVHLDLKSENIFLSESQKIKIGDFGLAKKYENSMSGVAGTYYYLSPEILLNKNYSRPADIFSLGCIFYEMYTLNLLPLTKRSFGQELIEGKFDRKAFKQEFDDSDEPIADLILDMLNLDQNLRPTIDLILQNKLLLGTGNFAKVYHGTEISTGDDVAIKVIKNYEVVKLIGVGGEAKALLVKRINSDKLYVMKQRMFFLLEEANEGLCEAMSLAKIQSPYIVRFEEVFLDNNSNMFSLCIVMEYCEGGDLMDNLFKRISDSFLKNSGLNNNQNNNNNNNNNLNSIVNNNILNNNKNSKQSTTNTNSSGSNSSIASNTTNNNNNNNNINNNNNLKAYENFFSKYVPSETLKGVNQLVEPNCNKDNNKPIEITTTDSPRIIINDNESTNNLNSQSDQILQFSISSESSSFSNNDLINSPNSNDSNLHQSSSNSSICGDYSSPPNLEKISSPGTSTPYQKGSNGNTVNIRCSDIVVEAVSPIKTESIGCQIRSTTSPCTSGPTSPQMIPLNIVEQPPQSTSTSKTDSSPTGADVKKTKMTWWKTYKSSKKDKKQTICSNACESFSNSTFFNDPSSHSQPQHQQLHSSPQQLPQSPRLKPNIESSLNINGNNNNNNCSTLLKLPRKLFYTWIYQICLGVQSIHKNHLVHLDLKSENIFLSESQKIKIGDFGLAKKYENSMSGVAGTYYYLSPEILLNKNYSRPADIFSLGCIFYEMYTLNLLPLTKRSFGQELIEGKFDRKAFKQEFDDSDEPIADLILDMLNLDQNLRPTIDLILQNKL",
    "response#3": "FRPDSVIGEGGFGYVYKGWIDERTLSPSKPGSGMVVAVKKLKEEGFQGHRQWLAEVDCLGRLHHMNLVKLIGYCSKGDHIRLLVYEYMPKGSLENHLFRRGAEPIPWRTRIKVAIGAARGLAFLHEAQVIYRDFKASNILLDSEFNAKLSDFGLAKVGPTGDRTHVSTQVMGTQGYAAPEYVATGRITAKSDVYSFGVVLLELLSGRLTVDKTKVGVERNLVDWAIPYLGDKRKVFRIMDTKLGGQYPHKGACLTANTALQCLNQEPKLRPKMSDVLSTLEELEMTGPSSTN"
  },
  {
    "reference": "MADMINITFPDGAVKEFPKGTTTAEIAGSISPGLKKKALAGMLDGTLLDLNTPIEQDGTITIVTPESDEALEVLRHSTAHVMAQALKRLFKDRNVKLGVGPVIEGGFYYDVDMDESLTPEDLPKIEKEMKKIIGENLPIERVVVSREEALARYEEVGDPYKIELINDLPEDETITIYEQGEFFDLCRGVHVPSTGKLKEFKLLNLAGAYWRGDSSNKMLQRIYGTAFFKKADLDEHLRLLEEAKERDHRKLGKELGIFALSQKVGQGLPLWLPKGATIRRIIERYIVDKEEKLGYQHVYTPVLASSELYKTSGHWDHYKDDMFPTMEMENEELVLRPMNCPHHMMVYKTEMRSYRQLPLRIAELGLMHRYEMSGAVSGLQRVRGMTLNDAHIFCRPDQIKDEFVRVVRLIQAVYEDFGLKNYSFRLSYRDPEDKEKYFDDDNMWNKAQGMLKEAMDELELEYFEAEGEAAFYGPKLDVQVRTALGKDETLSTVQLDFLLPERFDLTYVGEDGQPHRPVVVHRGVVSTMERFVAFLLEEYKGAFPTWLAPVQVQVIPVSPEAHLEYAKNVQETLQQAGIRVEIDERDEKIGYKIREAQMQKIPYMLVLGDKEVEANGVNVRKYGEKDSSSMGLDEFVRHVAREAKK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Also edits incorrectly charged L-seryl-tRNA(Thr)..2. The designed protein must possess metal ion binding, ATP binding, tRNA binding to support threonyl-tRNA aminoacylation under certain conditions.3. The protein should have a high affinity for Zn(2+). The designed protein sequence is ",
    "response#1": "WYPKGKLIRDLLSDYVYNLVVERGGMPVETPVMYDLQNNAIREHADKFGERQYRFKQGNKDLMLRFAACFGQFMMKKDMYLLPKHMPLKLYELSTYSFRYEQRGELVGLKRLRAFTMPDMHTVCIDMKQAMEAFEDQLWMGLKTGDDFKTPYAIIFRFTEDFFEENKEWFFGMAKEYKQKYGKDAILEILPGRKHYWVGKVDMAVVDSFGRPIENPTVQIDVESAERFGIVVHDGDKKVHPIILHCSPTGSVERVLCGLLENAYLNTLENRPPALL",
    "response#2": "WYPKGKLIRDLLSDYVYNLVVERGGMPVETPVMYDLQNNAIREHADKFGERQYRFKQGNKDLMLRFAACFGQFMMKKDMYLLPKHMPLKLYELSTYSFRYEQRGELVGLKRLRAFTMPDMHTVCIDMKQAMEAFEDQLWMGLKTGDDFKTPYAIIFRFTEDFFEENKEWFFGMAKEYKQKYGKDAILEILPGRKHYWVGKVDMAVVDSFGRPIENPTVQIDVESAERFGIVVHDGDKKVHPIILHCSPTGSVERVLCGLLENAYLNTLENRPPALYAIEADKKHLFLRFAACFGQFLMGHDMTFSYRSLPLRMFELTRYSFRREQRGELVGLRRLRAFTMPDMHTFCGDMSSAMEEFRKQYEASISVLRDAGLDLKDYEVAIRFTKDFYENNKTFIEGLVDIVDKPVLIEMWDERFFYFVLKFEFNFVDALDKASALSTVQIDVENAERYDIGYVDAEGNRQRPIILHCSPSGAIERLMYALLEG",
    "response#3": "WYPKGKLIRDLLSDYVYNLVVERGGMPVETPVMYDLQNNAIREHADKFGERQYRFKQGNKDLMLRFAACFGQFMMKKDMYLLPKHMPLKLYELSTYSFRYEQRGELVGLKRLRAFTMPDMHTVCIDMKQAMEAFEDQLWMGLKTGDDFKTPYAIIFRFTEDFFEENKEWFFGMAKEYKQKYGKDAILEILPGRKHYWVGKVDMAVVDSFGRPIENPTVQIDVESAERFGIVVHDGDKKVHPIILHCSPTGSVERVLCGLLENAYLNTLENRPPALH"
  },
  {
    "reference": "MTQLHKQIIILVACFALLLWLSQKISLPFNTERTERVPFNKLVELIEEEKIKTIELRGDNTLFAGLTDGSNIESRAIGNDSIITTLKNYDVPVEIIKQIAIDVKRENGFISFIAILIPTLFPILLIVGFFYLLYKQAQGFSSKTFSFGQSAGKQVNYEGTEGRSGAVKTKFKDVAGVKEAKEELMEVIDFLKNPKKFTDLGARIPRGVLLIGPPGCGKTLLAKAVAGEADVPFFHISGSEFMELFVGVGASRVRGLFQKAKRNAPCIIFIDELDSIGRKRGAGFGGAHDERDQTLNQILVEMDGFDTDTNVIVMAATNRPDVLDSALLRPGRFDRRITIDLPDIASREEMLKVHAKGKPLTDSVNLRRVAERTPGFSGADLAYLMNEAAILTARNNKTQITMEELLPSIEKVMLGPERRSRVITDQEKKITAFHEAGHAIVAHTLPEADPVHKVSVIARGQAGGYTLKIPEKDRYLHTRAEFMADLSVSMGGLAAEKIKFNEMTTGASGDLRYATQLARKLVTRYGMSDKIGPVSFELEDTNGFSQFPWEELKSVYSDTTLENIDTEVRRFLEEAYNNARKIIELHLEKLELVAEKLLEQETIEKEEFDELMQTA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Plays a role in the quality control of integral membrane proteins..2. The protein should have Helical domains to ensure proper insertion into the membrane.3. The protein design must be focused on enhancing the efficiency of cell division. The designed protein sequence is ",
    "response#1": "ILMVGPPGTGKTLLAKAIAGEAKVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVL",
    "response#2": "SMGVVLLLMTIIVKVLVYPATYKSYMSSAKMRVLKPYINEINAKYPKKEDALKKQQETMALYSKYGVSPMGGCLPMLIQMPVFMALFFFVPNAIELRQQSFLWAPDLSTYDDIINWGTNIPLLGNHLSLFCLLFSITNILNTMYTMKQQDMGQQQMPGMKLMMYIMPVMFIFIFNGYSSGLNYYYFISGLIGILTMVILL",
    "response#3": "IPKGVLLIGPPCTGKTLLAKAIAGEAGVPFFSISGSDFVEMFVGVGASRVRDMFEQGKKHAPCLIFIDEIDAVGRSRFSGIGGGHDEREQTLNALLVEMDGFETSEGVIIIAATNRADVLDPALLRPGRFDRQIIIDLPE"
  },
  {
    "reference": "MAPVTPDAVNARQQRPADPALRRLMHPHHRNYTASKASAHSVKSVSRCGKSRSELGRMERVGSVARSICSRHTRHGVDRSHFSLRDFFRGISANFELGKDFLREMNTPIHVSEAVFLPLSLCTLSPGRCLRLSPFGHSLTLGSHCEICINRSQVHVPQEFSSTQLSFFNNVHKIIPNKTFYVSLLSSSPSAVKAGLSQPSLLYAYLVTGHFCGTICPIFSTNGKGRLIMHLLLQGTSLHIPETCLKLLCENIGPTYELAVDLVGDAFCIKVSPRDTVYEKAVNVDEDAIYEAIKDLECGDELRLQIINYTQLILENKQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have metal ion binding within membrane, host cell nuclear inner membrane to regulate viral budding from nuclear membrane.2. The protein must exhibit the following characteristics: Within the host nucleus, NEC1 interacts with the newly formed capsid through the vertexes and directs it to the inner nuclear membrane by associating with NEC2; Induces the budding of the capsid at the inner nuclear membrane as well as its envelopment into the perinuclear space; There, the NEC1/NEC2 complex promotes the fusion of the enveloped capsid with the outer nuclear membrane and the subsequent release of the viral capsid into the cytoplasm where it will reach the secondary budding sites in the host Golgi or trans-Golgi network.; Plays an essential role in virion nuclear egress, the first step of virion release from infected cell. The designed protein sequence is ",
    "response#1": "MSPQTETKASVGFKAGVKEYKL",
    "response#2": "MGKRRGKS",
    "response#3": "KKKKKK"
  },
  {
    "reference": "MSKYIVVTGGVVSSIGKGITSASIGRILRSYGVDVTAIKIDPYLNWDSGTLNPYQHGEVFVTEDGMETDLDLGHYERFLDVNLSGKSNITTGKVYSSVIDHERKGDYLGSCVQIIPHITNKIKDMIRKIANESHADVVLVEVGGTVGDIESQPFLEALRQLRNEEGHDNVMVVHVTYVPYLKAAGEFKTKPTQHSTKELRSTGIIPDMIICRSELPMDQPLKNKISHFCDVERKAVINTPDVHSIYEVPLILNEEHVGEYVINRIKIETGEQDLDEWKKVVNALQQDEYQVNVGIIGKYVELEDAYMSIRESLKHAAAHLGIKVNIEWIQAENKINEKKISHLDSILIPGGFGERGISGKLEAVRYSLQHKVPLFGICLGMQCMVIEYARLNGLEDAHSTEFNLETPYPVIDLMEEQKKIKDIGGTMRLGSYTCQLKEGTMAKEAYGEEEVSERHRHRYELNNDYREILQEKGLIISGTSHDDFLVEMVELKDHPWFLGCQFHPEFKSRPNKAHPIFVSFMEATLLNHKQKSIN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein have Glutamine amidotransferase domains with specific sequence motifs that are crucial for its activity.2. The designed protein must possess metal ion binding, ATP binding, CTP synthase activity to support glutamine metabolic process under certain conditions.3. We require a protein that can specifically bind to the metabolites produced in the CTP from UDP: step 2/2, Pyrimidine metabolism pathway.4. The protein's active site, Nucleophile; for glutamine hydrolysis, Nucleophile must be carefully engineered to ensure that the amino acids involved in H2O + L-glutamine = L-glutamate + NH4(+) are properly positioned for optimal catalysis.5. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.6. The Mg(2+), ATP, CTP, L-glutamine, UTP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MPDAYLSLLESLTHAGIANDARVNIKWVNAEELAEGDLETQLGDADGILVPGGFGIRGIEGKIKAAEYARTHNVPYLGICLGMQIAVIEYARHVAGLTGANSAEFDPYAPHKVIDLMPEQLEVEGLGGTMRLGDWPMELRAGTKIAELYGVPQGGTVRERHRHRYEVNPAYVGQLQDAGLVISGVTPGVQGRGAGLVESIEIPGHPFFVALQAHPEFKSRPMRPSPPFAGFVEAAAQVAGAEVRIGLVGKYVELPDAYKSIIEAFIHAGAKNECKVKVITIQSGQLTTENAVEKLRGLHGVLVAPGFGERGFEGKIEAIRYVRENNIPFFGICLGMQCAVVEYGRNVLNLPDANSTEMNPETPNPVISIMEDQKNVTNMGGTMRLGAYPCDLKKGTKAHHAYGKTHISERHRHRYEFNNEYFKAYEDAGLVMSGINPENNLVEVIELKNHPYFIGAQFHPELKSTVANPHPLFVNFVAAALERRG",
    "response#2": "MPDAYLSLLESLTHAGIANDARVNIKWVNAEELAEGDLETQLGDADGILVPGGFGIRGIEGKIKAAEYARTHNVPYLGICLGMQIAVIEYARHVAGLTGANSAEFDPYAPHKVIDLMPEQLEVEGLGGTMRLGDWPMELRAGTKIAELYGVPQGGTVRERHRHRYEVNPAYVGQLQDAGLVISGVTPGVQGRGAGLVESIEIPGHPFFVALQAHPEFKSRPMRPSPPFAGFVTAAARFTGDLADAVVTIAVVGKYIDHRDAYKSIYESLDHAGFSHNSRVLVKRIEAEELEEQDPEIVLSGIDGLLVPGGFGYRGIEGKVKAIQFARECEIPYFGICLGMQCAVIEFARNRLGLAEANSSEFSAETPDPVICLLEEQKTVTDKGGTMRLGTQPCQLVENSRAAACYGTSEVSERHRHRYEFNPEYEQAFEKAGMIAAGKSPSGKLVEVVEIPAHPWFVAVQFHPEFKSKPHAAHPLFRGFLTAVGASITEPILSDSYISVIEAVKHASWNNNVDPEIQWIDSKIFEKKPEKLTILDELNAIIVPGGFGLSGIQGKIDTIRYAREHRIPYLGLCLGMQLAVVEYARNVCKLSNANSREIDKKAKHLVIDFIPEQVNIIRDSRYGATMRLGAYPAVLGKGTQVQKLYGKNKVYERHRHRYEVNPDYVKILEKKGLVFSGRSPDGVLMEFMELPGHSYFVATQAHPEFKSRPMKPAPLFDGLIGGRVKDLPGVRYHIVRGTLDASGVKERNQRRYGIKLAR",
    "response#3": "MPDAYLSLLESLTHAGIANDARVNIKWVNAEELAEGDLETQLGDADGILVPGGFGIRGIEGKIKAAEYARTHNVPYLGICLGMQIAVIEYARHVAGLTGANSAEFDPYAPHKVIDLMPEQLEVEGLGGTMRLGDWPMELRAGTKIAELYGVPQGGTVRERHRHRYEVNPAYVGQLQDAGLVISGVTPGVQGRGAGLVESIEIPGHPFFVALQAHPEFKSRPMRPSPPFAGFVDAYKSIYESLDHAGFSHNSRVLVKRIEAEELEEQDPEIVLSGIDGLLVPGGFGYRGIEGKVKAIQFARECEIPYFGICLGMQCAVIEFARNRLGLAEANSSEFSAETPDPVICLLEEQKTVTDKGGTMRLGTQPCQLVENSRAAACYGTSEVSERHRHRYEFNPEYEQAFEKAGMIAAGKSPSGKLVEVVEIPAHPWFVAVQFHPEFKSKPHAAHPLFRGFVEAAAKV"
  },
  {
    "reference": "MEDDGGERSSFVAGLIENRAKEVGMAAFDLRSASLHLSQYIETSSSYQNTKTLLRFYDPSVIIVPPNKLAADGMVGVSELVDRCYSTVRKVVFARGCFDDTKGAVLIQNLAAEEPLALGLDTYYKQHYLSLAAAAATIKWIEAEKGVIVTNHSLTVTFNGSFDHMNIDATSVENLELIDPFHNALLGTSNKKRSLFQMFKTTKTAGGTRLLRANLLQPLKDIETINTRLDCLDELMSNEQLFFGLSQVLRKFPKETDRVLCHFCFKPKKVTEAVIGFENTRKSQNMISSIILLKTALDALPILAKVLKDAKCFLLANVYKSVCENDRYASIRKKIGEVIDDDVLHARVPFVARTQQCFALKAGIDGFLDIARRTFCDTSEAIHNLASKYREEFNLPNLKLPFNNRQGFFFRIPQKEVQGKLPNKFTQVVKHGKNIHCSSLELASLNVRNKSAAGECFIRTETCLEALMDAIREDISALTLLAEVLCLLDMIVNSFAHTISTKPVDRYSRPELTDSGPLAIDAGRHPILESIHNDFVSNSIFMSEATNMLVVMGPNMSGKSTYLQQVCLVVILAQIGCYVPARFATIRVVDRIFTRMGTMDNLESNSSTFMTEMRETAFIMQNVTNRSLIVMDELGRATSSSDGLAMAWSCCEYLLSLKAYTVFATHMDSLAELATIYPNVKVLHFYVDIRDNRLDFKFQLRDGTLHVPHYGLLLAEVAGLPSTVIDTARIITKRITDKENKRIELNCGKHHEIHRIYRVAQRLICLKY",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The ATP binding site should be stable and able to withstand changes in environmental conditions.2. The protein must exhibit the following characteristics: Promotes homologous recombination through facilitating chiasma formation during prophase I; Involved in meiotic recombination in association with MSH5; Involved in the control of class I crossovers formation..3. A protein that contributes to reciprocal meiotic recombination, meiotic mismatch repair involved in reciprocal meiotic recombination, homologous chromosome pairing at meiosis by functioning in plasmodesma, condensed nuclear chromosome with ATP-dependent DNA damage sensor activity, double-stranded DNA binding, mismatched DNA binding, ATP binding. The designed protein sequence is ",
    "response#1": "YASRTHSQLSQAMQELKNTSYLFVRSIILGSRDQLCIHPDISKQENNAIKTVLCRESVKARNCSFYNRVETAKDRPDVATVPVMDIEDLVTVGRKLKACPYYLSKELVEQADVIFMPYNYLLDPKARKSNGLSLQNTVIILDEAHNVEKMCEEVGSALLRSSS",
    "response#2": "YPLLNKCSEEWQASGIWGQISRLKQIFVEPRGKDQFTTTMAEYYAQVRDPASRGAIFMAVCRGKVSEGLDFADANGRAVMITGLPFPPMMDARVVLKKQYLDTNRTRENELITGNDWYSLEASRAVNQAIGRVIRHKDDYGAILLCDSRFQR",
    "response#3": "LSLCPIIPGGLLIFFPSYPLLNKCSEEWQASGIWGQISRLKQIFVEPRGKDQFTTTMAEYYAQVRDPASRGAIFMAVCRGKVSEGLDFADANGRAVMITGLPFPPMMDARVVLKKQYLDTNRTRENELITGNDWYSLEASRAVNQAIGRVIRHKDDYGAILLCDSRFQNARQQAQLSAWIHR"
  },
  {
    "reference": "MSEEDKKDPPKSIEDLMKLLSMGESLTEPQQKQMKDYKFWKTQPVPSFDEKIDSEGPIDQTKTPDDIPDTPLPLLNEFEWSTVDLEKTDQLDEVYKLLYENYVEDQDATFRFKYSHEFFNWALKPPGWRTEWHAGVRVKETGKLVAFIAAIPITLKLNKSQKVIPSVEINFLCVHKKLRSKRLAPVMIKEITRRVNKYDIWQALYTGGVVLPSPVATCRYTHRPINWSKLYDVGFSHLPANQTKSGMLANYALPNTTKTQGLRSMKIEDLDQVYDLLHKFQEKFDIVQVFEKDELAHWLLGGKSTDTSSNVIKSYVVENSEGSLTDFFSYYLLPFTVLDNPSHDELGIAYLYYYASDTAFKNDDESAYKQRLSSLMSDALVTSKKYNVDVFNCLSSQDNPYFIKNCKFGSGDGFLNYYLFNYKTWPITGGIDPQTKELVEDKSSGVGVILL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins..2. The Proton acceptor; via carboxylate active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. The designed protein should have a high specificity for tetradecanoyl-CoA binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "IVIAGIAGRLPESDNLEEFWHNLVNGIDMVTDDGRRWKPGLYGLPHRSGKLKDISKFDASFFGVHPKQAHTMDPQLRIILEVAYEAIVDGGINPAALRGTNTGVWIGVSGSEAAEAFSRDPEELLGYSMTGCQHAMFANRLSYFFDLKGPSSSIDTACSSSLLALENAFQAIRHGQCDSALVGGVNLLLKPNTSVQFMKLGMLSPDGSCKSFDASGNGYCRSEAAVVVLLTKRSAAKRVYATVVNAGSNTDGYKEQGVTYPSGEMQQRLMTAVYEEAGISPADVEYIEAHGTGTKVGDPQELNGIVNAFCQSDRKPLLLGSTKSNMGHPEPASGLAALAKVVLSLEHGLWAPNIHYKTPNPAVPALQNGCIHVVTEPIPVKGGVVGVNSFGFGGSNVHVILRPPWREAATVLLAAGGGAGGASPRGCDYRVLLLRRSPRSAFMPSAHVFPGGVADAADFSPEWWTVLPGPAPLCGLRPKRGPRAPLFAAERPELGQALPGDLAFRLCAIRETFEEAGLLLLAEPDQAQAAIEAFSSSEGPAPCVLPPERLPLPGPELAEWRRRVQRQPSLFLALCRRLGLVPNIWALSEWSNWLTPRALAGKGGRRYDTAFFLCCLAHAAPEHPHGAHEPDGDGEVTACQWSSPSEAFELFKSQDIWIAPPQFH",
    "response#2": "MEDIVIAGIAGRLPESDNLEEFWHNLVNGIDMVTDDGRRWKPGLYGLPHRSGKLKDISKFDASFFGVHPKQAHTMDPQLRIILEVAYEAIVDGGINPAALRGTNTGVWIGVSGSEAAEAFSRDPEELLGYSMTGCQHAMFANRLSYFFDLKGPSSSIDTACSSSLLALENAFQAIRHGQCDSALVGGVNLLLKPNTSVQFMKLGMLSPDGSCKSFDASGNGYCRSEAAVVVLLTKRSAAKRVYATVVNAGSNTDGYKEQGVTYPSGEMQQRLMTAVYEEAGISPADVEYIEAHGTGTKVGDPQELNGIVNAFCQSDRKPLLLGSTKSNMGHPEPASGLAALAKVVLSLEHGLWAPNIHYKTPNPAVPALQNGCIHVVTEPIPVKGGVVGVNSFGFGGSNVHVILRPWREAATVLLAAGGGAGGASPRGCDYRVLLLRRSPRSAFMPSAHVFPGGVADAADFSPEWWTVLPGPAPLCGLRPKRGPRAPLFAAERPELGQALPGDLAFRLCAIRETFEEAGLLLLAEPDQAQAAIEAFSSSEGPAPCVLPPERLPLPGPELAEWRRRVQRQPSLFLALCRRLGLVPNIWALSEWSNWLTPRALAGKGGRRYDTAFFLCCLAHAAPEHPHGAHEPDGDGEVTACQWSSPSEAFELFKSQDIWIAPPQFPPTQRRPRL",
    "response#3": "DPLKVLQLETVEVPAEPGSGECLVEWLASPINPLDINRIQGNYAVRAELPVIGGSEGVGRVVKAGSGSRFKSGDHVTIFSANTPIWTEFGVVDDDELVKLDNRIPLDLAATLMINPPTAWIMLKKYVNLQKGDYIIQNSANSGVGRSVIEMCKALGYKSINIVRNRQNIEALKTDLWRIGADHVFTEEEFKGTSRQFLKSINVRPKLALNGVGGKSALQISSVLERGGTCVTYGGMSKKAHEFTTSALVFNDICVRGVAVGMWARQEEHLDEWNLCVDEVQKLAVAGKITAIPMEKVVLADHKTAIQKSLEGRSIKQLL"
  },
  {
    "reference": "MTSPSAPSSAERPRLPGALDGPRLAAAAAEHGTPLWLYDAATIRAQIDRLRRFDVIRYAQKACSNLHILRLMREEGVHVDAVSEGEIERALAAGYRVGGDDEPIVFTADLLNRSTLRRVVELGIPVNAGSPQMLDQVGRAAPGHPVWIRINPGFGHGHSRKTNTGGEHSKHGIWHEHLEESLALVDRHGLDLVGLHMHIGSGVDYGHLESVCETMVKQVRMAGRDIRAISAGGGLSVPYTPGDPEIDTDRYFELWDAARRELVSELGHPVRLEIEPGRFLVAGAGVLAAEVRAQKPVGSNYFVLVDAGFNDLMRPAMYGSNHRVSVLDADGAPRASDARDTVLAGPLCESGDVFTQVEGGDVEPVPVPRTDVGDLVVFHDTGAYGASMSSTYNSRPLIPEVLVDGAETRLIRRRQTVAELLAPELEPGPALSPRPSRDPR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit diaminopimelate decarboxylase activity.2. A protein should be able to manipulate the activity of enzymes in the L-lysine from DL-2,6-diaminopimelate: step 1/1, Amino-acid biosynthesis pathway, either by activating or inhibiting their function.3. pyridoxal 5'-phosphate is required for the proper folding and stability of the protein.4. The substrate, pyridoxal 5'-phosphate binding site should be stable and able to withstand changes in environmental conditions.5. We are looking for a protein with broad substrate specificity for H(+) + meso-2,6-diaminoheptanedioate = CO2 + L-lysine. The designed protein sequence is ",
    "response#1": "FWVYDADIIYKKINLLRKFDVIRFAQKSCSNINILRLMKNKNVKIDAVSLGEIERALLSGFKPSTNEIIFTADILDEETLLKVAKYKIPVNAGSLDMLKQLGKISPGHHVWLRINPRFGYGHSKKTNTGGENSKHGIWHPEQAIPIIKKYKLKLIGLHMHIGSGVNYLHLKKVSQAMIKYAFQLNQKISSISVGGGLPIPYKFNDQPIDIKKYFMLWDIARKKISKFLGEKIELEIEPGRFLVAESGILISKVWATKKMGNKNFVLVDVGFNDLMRPTMYGSYHHISVIYGDDRKMNEKETIDTVVAGPLCESGDIFTQKEGGTVQTRKLPTIKVGDYLIFHDTGQLTP",
    "response#2": "FWVYDADIIYKKINLLRKFDVIRFAQKSCSNINILRLMKNKNVKIDAVSLGEIERALLSGFKPSTNEIIFTADILDEETLLKVAKYKIPVNAGSLDMLKQLGKISPGHHVWLRINPRFGYGHSKKTNTGGENSKHGIWHPEQAIPIIKKYKLKLIGLHMHIGSGVNYLHLKKVSQAMIKYAFQLNQKISSISVGGGLPIPYKFNDQPIDIKKYFMLWDIARKKISKFLGEKIELEIEPGRFLVAESGILISKVWATKKMGNKNFVLVDVGFNDLMRPTMYGSYHHISVIYGDDRKMNEKETIDTVVAGPLCESGDIFTQKEGGTVQTRKLPTIKVGDYLIFHDTGRGDIDMEVIREEIIRNIHEIQQAFAAHKNTKTFFASKTCSVMGVLKAIRDAGICAEANSQYEVRKCLEIGFRGDQIVFNGVVKKPADLEYAIANDLYLINVDSLYELEHIDAISRKLKKVANVCVRVEPNVPSATHAELVTAFHAKSGLDLEQAEETCRRILAMPYVHLRGLHMHVGDQVPESEPFAKATKVLVDESRRLEEVLGIKFDLINVGGGIPVPYKYDDENGDPLKDNMYAGITAQDFADAVIREVHKWRTDVEICIEPGRKVEL",
    "response#3": "FWVYDADIIYKKINLLRKFDVIRFAQKSCSNINILRLMKNKNVKIDAVSLGEIERALLSGFKPSTNEIIFTADILDEETLLKVAKYKIPVNAGSLDMLKQLGKISPGHHVWLRINPRFGYGHSKKTNTGGENSKHGIWHPEQAIPIIKKYKLKLIGLHMHIGSGVNYLHLKKVSQAMIKYAFQLNQKISSISVGGGLPIPYKFNDQPIDIKKYFMLWDIARKKISKFLGEKIELEIEPGRFLVAESGILISKVWATKKMGNKNFVLVDVGFNDLMRPTMYGSYHHISVIYGDDRKMNEKETIDTVVAGPLCESGDIFTQKEGGTVQTRKLPTIKVGDYLIFHDTGFSEPF"
  },
  {
    "reference": "MKQLIIDLIKLYRYSIGLLIPPSCRFYPTCSNYMHEALVKHGLIKGLWLGMKRILRCHPWNQGGYDPVP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Could be involved in insertion of integral membrane proteins into the membrane.2. The designed protein should be expressed specifically in the plasma membrane The designed protein sequence is ",
    "response#1": "MS",
    "response#2": "MTFLP",
    "response#3": "EAGSKR"
  },
  {
    "reference": "MADAQMQVAPTPTIQMRTEEDLSLPHPSAPEGLPPPPPKETFSKFQQQRQASELRRLYKHIHPELRKNLEEAVAEDLAEVLGSEEPTEGDVQCMRWIFENWRLDAIGDHERPAAREPVSGGNVQATSRKFEEGSFTNSSDQEPEGLRPSGGDVQAARQMFETKPLDALRGQEEATQTTMREPAATGDVQGTRKLFETRPLDRLGSRPSIQEQSPLELRSEIQELKGDVKKTVKLFQTEPLCAIQDAEGTIHEVKAACREEIQSNAVRSARWLFETRPLDAFNQDPSQVRVIRGISLEEGALPDVSATRWIFETQPLDAIREIEVDEKDFQPSPDLIPPGPDVQHQRHLFETCSLDTLKGERETEAEVPPKEEVIPGDVRSTLWLFETKPLDAFRDQVQVGHLQRVGHQEGEGLVTECLPSNGTSVLPLSQGVPQNDGLKGDVKTFKNLFETLPLDSIGQGEPSAYGNINRGQNTDSAEQSQGSDAPVYAMQDSRGQLHALTSVSREQVVGGDVQGYKWMFETQPLDTLGRSPSTIDVVRGITRQEVVAGDVGTTRWLFETQPLEMIHQQEQQKPEEEEGKGPGGPPPELPKKGDVQTIRWLFETYPMSELAEKRESEVTDPVSKAETQSCTWMFGPQSLNPAEGSGEQHLQTSQVPAGDRQTDRHVFETESLPASNQSSGRKPVRYCSRVEIPSGQVSRQKEVFQALEAGKKEVPETTINLGSIPTGSVHKFTWLFENCPMGSLAAESIRGDNLQEEQPKGSAGHGTP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that can perform filamin binding in adherens junction, stress fiber, fascia adherens, focal adhesion to support negative regulation of cell population proliferation, actin filament organization. The designed protein sequence is ",
    "response#1": "EEYERLASDLLEWIRRTMPWLNSRQSDSTLAGVQKKLEEYRTYRRKHKPPRVEQKAKLETNFNTLQTKLRLSNRPAYMPTEGKMVSDITNSWKGLEHAEKAFEEWLLAETMRLERLEHLAQKFKHKADTHEDWTKGKEEMLQSQDFRNCKLNELKALKKKHEAFESDLAAHQDRVEQIAAIAQELNTLEYHDCASVNARCQRICDQWDRLGALTQRRRQGLDEC",
    "response#2": "EFAKRAAPFNNWLDGAREDLVDMFIVHTMEEIQGLIQAHDQFKATLGEADKEFNVIIGLVRDAEAIVKQEQVPGGLVNPYTTLSADLISRKWSEVRALVPQRDQTLANELRKQQNNEMLRRQFAEKANAVGPWIERQMDAVTAIGMGISGSLEEQLHRLKEYEQAVYAYKPSIEELEKIHQAVQESMIFENRYTHYTMETLRVGWEQLLTSINRNINEVENQC",
    "response#3": "RRQFAEKANAVGPWIERQMDAVTAIGMGISGSLEEQLHRLKEYEQAVYAYKPSIEELEKIHQAVQESMIFENRYTHYTMETLRVGWEQLLTSINRNINEVL"
  },
  {
    "reference": "MQNFFNFTLFELEEYMLELGLDKYRARQLFNFIYAKGFDEFLDISVLKKTDRNMLSEKLFIPKLESSLIKDTDGTTKFLFKLLDGQAVESVLIPMKEEKNTICVSTQVGCKMNCKFCATAKLGFIRNLQPWEIVYQVRYIYNQIKKETYKTPNIVFMGMGEPLDNYENVIKAVLIINNEYGLSISRRRITISTCGIVPKIEELKRDLAYINLAVSLNAADNLKRSYLMPINNSYPIEELINSVKDFPLPERKRLTFEYIMIKGFNDTQEDIKNLVKLLKPIKCKLNLIPLNKHLYSENLYPSSIQTIENFAKQLRDKNMFVTVRKSKGKSINAACGMLVATMR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The S-adenosyl-L-methionine, [4Fe-4S] cluster binding site should be located in a region of the protein that is accessible to the ligand.2. A protein that can perform 4 iron, 4 sulfur cluster binding, tRNA (adenine-C2-)-methyltransferase activity, rRNA binding, tRNA binding, metal ion binding in cytoplasm to support rRNA base methylation.3. For general function, the protein need meet that m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.4. The protein must be engineered to have a high affinity for [4Fe-4S] cluster in order to increase its catalytic activity.5. The Proton acceptor, S-methylcysteine intermediate active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.6. The designed protein must have at least one Radical SAM core domain. The designed protein sequence is ",
    "response#1": "MTACVSSQVGCSLSCKFCATGYMERKRNLNPDEIYDQVVLIAKQANDNYQMPLSNIVYMGMGEPLLNYSNVMKSIERITAEDGLNMAAKRITVSTAGIAKMIKKLGDDQVRFNLALSLHAANDEKRNTIMPINEQNSLKALAEALKYYFAKTKNPVTYEYIVFDNFNDGIEDAIELAKFCRHLPCKVNIIEYNPISFAAYQNAETDKIDAFADYLSAEEQRAAEEAKPSLIGLSRAEIAAELGRIGVAPAQRKMRSQQLWHWMYLRGAREFSEMSSISKDMRAELEKHFTVARPEVVAEQISNDGTRKWLLRLPSGTQGEKAHEVECVYIPETDRGTLCVSSQVGCTLTCSFCHTGTQRLVRNLTPGEIVGQIMVARDRLNDWADRDTANGNRLVTNVVMMGMGEPLYNFEAVRDALLIVADNEGIGISRRRITLSTSGVVPNIYRTGDEIGVQLAISLHAVRDELRNELVPINKKYPLEQLLQACRDYPGASNARRITFEYVMLRGINDSLEDAKLLVKMLKGIHAKINLIPFNPWPGTRYECSDWDQIEKFSEYVFNAGYSSPVRTPRGRDILAACGQLKSETEKLSARERDTLLSRLS",
    "response#2": "MTACVSSQVGCSLSCKFCATGYMERKRNLNPDEIYDQVVLIAKQANDNYQMPLSNIVYMGMGEPLLNYSNVMKSIERITAEDGLNMAAKRITVSTAGIAKMIKKLGDDQVRFNLALSLHAANDEKRNTIMPINEQNSLKALAEALKYYFAKTKNPVTYEYIVFDNFNDGIEDAIELAKFCRHLPCKVNIIEYNPISFAAYQNAETDKIDAFADYLEVRTRKATTTG",
    "response#3": "MTACVSSQVGCSLSCKFCATGYMERKRNLNPDEIYDQVVLIAKQANDNYQMPLSNIVYMGMGEPLLNYSNVMKSIERITAEDGLNMAAKRITVSTAGIAKMIKKLGDDQVRFNLALSLHAANDEKRNTIMPINEQNSLKALAEALKYYFAKTKNPVTYEYIVFDNFNDGIEDAIELAKFCRHLPCKVNIIEYNPISFAAYQNAETDKIDAFADYLSQEGPNVSWVYPERVTMCISSQAGCGMNCPFCATGQAGLTRNLSTAEIVEQVLAGSRSLDRGQIAGGPGRVSNVVFMGMGEPMANYGAVVESVRRLISPAPSGLGLSARGITVSTVGLVPRMKDLATEGLPVTLALSLHAPDDELRNTLVPVNTRWPVAEVLNAAWEYADRTHRRISIEYALIRDINDQAWRAELLADLLVGHLVHVNLIPLNPTPGSIWTASRPADEETFVRILRDRGLAVTVRGTRGRESRGGRLLRP"
  },
  {
    "reference": "EVLKQLQGSIEDEAMASSGQIDLLERLKELNLDSSNFPGVKLRQKVSLRSFGSREGSVSSRSGECSPVPVGSFPRRGFMNGSRESTGYLEELEKERSLLLAELEKEEKEKDWYYAQLQNLTKRIDSLPLTENFSLQTDMTRRQLEYEARQIRAAMEEQLGTCQDMEKRAQARVARIQQIEKDMLRIRQHVQSQAAEAEKTSQTKHDPASREAERQNEGQGAAESSTGTTGSSQGSGTRMDHDTASVMSSSSTYSVPRRLTSHLGTKVTEDYKPQVATLHFYIALAPLNTSIILTTYLCKIMLSSNCSSMFTVFCHSSHHRFVQLPSLAGGQKHRDLSQISLHINVHPHLCLVRFLCCHVSDLHLLKHPIMASATSWETAPYLPLETFIVAGFQIQGYPLLTPPPPQKKSCYVSGPYKTTHLHRLNWGGGVQLYSEPQSHTLVLKNMTHFVFLFKLSIYFTLVIILETWSQGLNKSLNCMVINQLKALFKKKKKENEAFFLLMVTIASVCRSLSEPEALHFCKDWPWDFSLYIHTQCPKSWLKNLKSMRQCCCNIYITTTTKKNLLINRDNGRGIERTERFVSNMLAIVTLGKGWRKKLAIFVVFFSPRRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNAKDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLSETFDNIDNLSPKTSHRNKQRQKQNLYSEYALDSGRHNDDSIC",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Ensure the presence of a signal peptide within the protein.2. The designed protein should have microtubule binding, beta-catenin binding to facilitate retina development in camera-type eye, heart jogging, negative regulation of canonical Wnt signaling pathway involved in heart development.3. The designed protein's functionality will benefit from Basic and acidic residues, Polar residues compositional bias in its structure.4. The designed protein must have at least one EB-1 binding, Adenomatous polyposis coli protein basic domain. The designed protein sequence is ",
    "response#1": "NFKTLSTMEDFWKFTEGSLLDGLYWEMWYSNRTGAENRSFIYYENLLLGVPRIRQLKVRNGSCSIPPDLREEIKECYDVYSISNEERAPFGLRHGTAWTYTNEKDLNGSSHWGLIATYSGAGYYLDLSRTREETAVQIANLKKNIWLDRGTRATFIDFSVYNANINLFCVVRLLVEFPATGGVITSWQFQPVKLA",
    "response#2": "TLVECVGKGRYGEVWRGQWQGENVAVKIFSSRDEKSWFRETEIYNTVLLRHENILGFMASDMTSRNSSTQLWLITHYHENGSLYDYLQRVAVETVDGLLMATSVVSGLVHLHTEIFGTEGKPAIAHRDLKSKNILVKKDLRCCIADLGLAVTHSQTDNQLDIGNNPKVGTKRYMAPEVLDESIQTDCFDAYKRVDIWAFGLVLWEIARRTYSNGIVEEYKPPFYDLVPNDPSFEDMRKVVCVEHQRPFIPNRWFSDPTLSALAKLMKECWYQNPSARLTALRIKKC",
    "response#3": "FAQPYEPRVCENAAQGKLVVQISAIDKDITPRDVKFKFSLTTEDSNFTLTDNHDNTANITVKYGQFDRERAKVHHLPVLISDNGRPSLTGTSTLVVTICKCNEHGEFC"
  },
  {
    "reference": "MAKYVFITGGVVSSIGKGIVAASLGRLLKSRDYSVSILKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTDTSMSRLNSVTTGSIYQAVINKERRGDYQGGTVQVIPHITNEIKERIHRVARNTGSDVVITEIGGTVGDIESLPFLEAIRQFRKDVGRNNVVYMHVTLIPWISAAGEMKTKPTQHSVKELRSIGIQPDVLVCRSQRPLKSGIKEKISEFCDVPVESVVTSRDASSIYEVPLILEQEGLAQQTLELLQLDRRKPDLDGWSNLVARMNSPKTKIEIAIVGKYVQLSDAYLSVVEALGHAAIATGSEVNLRWVSAEDLGKHGAEQYLKDVAGIVVPGGFGIRGVDGKVAGIGYARENKIPFLGLCLGMQCSVIEWARNIAHLERANSSEFDEKTPNPVINLLPEQHDVVDLGGTMRLGLYPCRLKPNTLAFSLYQEEVVYERHRHRYEFNNAYRSLLLETGYEVSGSSPDGRLVEIIELKGHPFFLATQFHPEFRSRPSNPHPLFLGFIQAAIGGETVNYSLEEINLELKS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design must be able to perform glutaminase activity in order to contribute to 'de novo' CTP biosynthetic process.2. The Nucleophile; for glutamine hydrolysis of the protein must be engineered to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).3. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen4. The protein design should take into account the physiological conditions of the CTP from UDP: step 2/2, Pyrimidine metabolism pathway. The designed protein sequence is ",
    "response#1": "LADAYKSLNEALYHAGLHTHTRVRIRYIESETLEQQGTRVLQGNDAILVPGGFGERGIEGKILAARYARENKIPYLGICLGMQVAVIEYARNVCGLTGAHSTEFNPSSAHPVIGLVTEWIDKTGKVERRTEKSDLGGTMRLGIQNCRLRSGSRSRALYNAEVIAERHRHRYEFNNNYRDQLTGKGLAIVGVSENEELVEMIELSDHPWFVACQFHPEFTSTPRDGHPLFEGFVKSVQG",
    "response#2": "LQDAYKSLNEALVHGGIANRVKVNIRWIDAEIFEHEDSDVAASLEPLHAILVPGAFGERGAEGKIASVKFARERQVPYFGICFGMQMACIEGARNLAGIEKASSTEFGPTDEPVVGIITEWMTAEGVEKREAGGDLGGTMRLGAYDAKLAGNSVVSSIYGATDISERHRHRYEVNTGYRTALEKGGLVFSGMSPDGMLPEIVERPDHPWFVGVQFHPELKSKPFDPHPLFASFIVR",
    "response#3": "LPDAYLSVTEALRAGGFANNARVTIKWVTSDDCKTPAGAAAQLSGVDAVCVPGGFGDRGVEGKVSAITFARENRIPLLGLCLGLQCIVIEAARNLAGIDDANSTEFDPATAHPVISTMAEQLDIVGGKGDLGGTMRLGTYPAKLAEGSVVREAYSDQPYVEERHRHRYEVNNAYRAELEKKAGIVFSGTSPDNKLVEFVEYPREVHPYLVATQAHPELKSRPTRPHPLFAGLVAVNGL"
  },
  {
    "reference": "MAKQKVPAAPSVRRLPSYLHLVKKAEADKLEYISGTVIAEELELEPIQVRKDLTITGIVGKPKKGYPVKLLITAIEKFLGWNKEKKAFVIGAGSLGTALSGYQGFKEHGLDICAAFDSDKRKIGKEIHELPVFGMDELETKVKEYKPEIAILTVPSKYAQEAANAIVKAGIKAIWNFTNIKITVPDKVIVQKEDLSSGYAMLGVMMNTKK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The NAD(+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "LPFDVVNKIPNFLPNYHKYKVGSVLVIGGSLKYTGAVALAAKAAKAAGSGMVFALVDKSIHSIVASKLLEEVVDFYDKSNYEDILNSYYSKVSVIVFGNGIEDNSINFNILKHILTNFEGVLIIDGTGINMFYDYFIKMKNEFSTKVSRIVLTPHSGELSLFFSKASDKVKVSNLTDFDRLSSSLRLANLIPNSVVVSKGNPTFISYYANGVDNVWINLTNGLALAKAGSGDVLAGIIGAFASMLKNSISKNLIKDEVVEAIKMGVFIHGLSGQLAKKELTTFSVSSEQIINYIPKAYSFS",
    "response#2": "LPFDVVNKIPNFLPNYHKYKVGSVLVIGGSLKYTGAVALAAKAAKAAGSGMVFALVDKSIHSIVASKLLEEVVDFYDKSNYEDILNSYYSKVSVIVFGNGIEDNSINFNILKHILTNFEGVLIIDGTGINMFYDYFIKMKNEFSTKVSRIVLTPHSGELSLFFSKASDKVKVSNLTDFDRLSSSLRLANLIPNSVVVSKGNPTFISYYANGVDNVWINLTNGLALAKAGSGDVLAGIIGAFASMLKNSISKNLIKDEVVEAIKMGVFIHGLSGQLAKKELTTFSVSSEQIINYIPKAYSFLFP",
    "response#3": "LPFDVVNKIPNFLPNYHKYKVGSVLVIGGSLKYTGAVALAAKAAKAAGSGMVFALVDKSIHSIVASKLLEEVVDFYDKSNYEDILNSYYSKVSVIVFGNGIEDNSINFNILKHILTNFEGVLIIDGTGINMFYDYFIKMKNEFSTKVSRIVLTPHSGELSLFFSKASDKVKVSNLTDFDRLSSSLRLANLIPNSVVVSKGNPTFISYYANGVDNVWINLTNGLALAKAGSGDVLAGIIGAFASMLKNSISKNLIKDEVVEAIKMGVFIHGLSGQLAKKELTTFSVSSEQIINYIPKAYSFLFP"
  },
  {
    "reference": "MDHSAEKAAANAEVPQELLEEMLWYFRAEDAAPWNYSILVLAVLVVMTSMFLLRRSILANRNRKKQPQDKETPEDLHLDDSIMKENNSQVFLRETLISEKPDLAPGEPELKEKDSSLVFLPDPQETES",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be designed to catalyze the following reaction dehydroepiandrosterone 3-sulfate(out) = dehydroepiandrosterone   3-sulfate(in).2. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.3. For general function, the protein need meet that Modulates SLC51A glycosylation, membrane trafficking and stability activities.; Can also transport steroids such as estrone 3- sulfate and dehydroepiandrosterone 3-sulfate, therefore playing a role in the enterohepatic circulation of sterols ; Essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood; Taurine conjugates are transported more efficiently across the basolateral membrane than glycine-conjugated bile acids; Able to transport eicosanoids such as prostaglandin E2 ; The Ost-alpha/Ost-beta complex efficiently transports the major species of bile acids (taurocholate)4. The Extracellular, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.5. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "KPGLNAILGPTGGGKSSLLDVLAARKDPHGLSGDVLINGAPRPANFKCNSGYVVQDDVVMGTLTVRENLQFSAALRLPTTMTNHEKNERINMVIQELGLDKVADSKVGTQFIRGVSGGERKRTSIAMELITDPSILFLDEPTTGLDSSTANAVLLLLKRMSKQGRTIIFSIHQPRYSIFKLFDSLTLLASGRLMFHGPATYKNLRSKSGIKVFRFIAPLYYINKECFKSALYKKTLNPVLVKAAWKKAAKRKLKEETVTFHGDPDEVSMQLSHDPLELHTVVIDCSAIQFLDTAGIHTLKEVRRDYEAIGIQVLLAQCNPSVRDSLAKGEYCKKEEENLLFYSLSEAVAFAKSSSARFLAALSFSYIAKALGGIIMKISITQIERRFDISSSLAGLIDGSFEIGNLLVIVFVSYFGSKLHRPKLIGIGCLLMGTGSILTSLPHFFMGYYRYSKETHINPSENSTSSLSTCLINQTLSFNGTSPEIVEKDCVKESGSHMWIYVFMGNMLRGIGETPIVPLGISYIDDFAKEGHSSLYLGSLNAIGMIGPVIGFALGSLFAKMYVDIGYVDLSTIRITPKDSRWVGAWWLGFLVSGLFSIISSIPFFFLPKNPNKPQKERKISLSLHVLKTNDDRNQTANLTNQGKNVTKNVTGFFQSLKSILTNPLYVIFLLLTLLQVSSFIGSFTYVFKYMEQQYGQSASHANFLLGIITIPTVATGMFLGGFIIKKFKLSLVGIAKFSFLTSMISFLFQLLYFPLICESKSVAGLTLTYDGNNSVASHVDVPLSYCNSECNCDESQWEPVCGNNGITYLSPCLAGCKSSSGIKKHTVFYNCSCVEVTGLQNRNYSAHLGECPRDNTCTRKFFIYVAIQVINSLFSATGGTTFILLTVKIVQPELKALAMGFQSMVIRTLGGILAPIYFGALIDKTCMKWSTNSCGAQGACRIYNSVFFGRVYLGLSIALRFPALVLYIVFIFAMKK",
    "response#2": "FLAALSFSYIAKALGGIIMKISITQIERRFDISSSLAGLIDGSFEIGNLLVIVFVSYFGSKLHRPKLIGIGCLLMGTGSILTSLPHFFMGYYRYSKETHINPSENSTSSLSTCLINQTLSFNGTSPEIVEKDCVKESGSHMWIYVFMGNMLRGIGETPIVPLGISYIDDFAKEGHSSLYLGSLNAIGMIGPVIGFALGSLFAKMYVDIGYVDLSTIRITPKDSRWVGAWWLGFLVSGLFSIISSIPFFFLPKNPNKPQKERKISLSLHVLKTNDDRNQTANLTNQGKNVTKNVTGFFQSLKSILTNPLYVIFLLLTLLQVSSFIGSFTYVFKYMEQQYGQSASHANFLLGIITIPTVATGMFLGGFIIKKFKLSLVGIAKFSFLTSMISFLFQLLYFPLICESKSVAGLTLTYDGNNSVASHVDVPLSYCNSECNCDESQWEPVCGNNGITYLSPCLAGCKSSSGIKKHTVFYNCSCVEVTGLQNRNYSAHLGECPRDNTCTRKFFIYVAIQVINSLFSATGGTTFILLTVKIVQPELKALAMGFQSMVIRTLGGILAPIYFGALIDKTCMKWSTNSCGAQGACRIYNSVFFGRVYLGLSIALRFPALVLYIVFIFAMKKLVPEVPTYKNLRSKSGIKVFRFIAPLYYINKECFKSALYKKTLNPVLVKAAWKKAAKRKLKEETVTFHGDPDEVSMQLSHDPLELHTVVIDCSAIQFLDTAGIHTLKEVRRDYEAIGIQVLLAQCNPSVRDSLAKGEYCKKEEENLLFYSLSEAVAF",
    "response#3": "KPGLNAILGPTGGGKSSLLDVLAARKDPRGLSGDVLINGAPQPANFKCSSGYVVQDDVVMGTLTVRENLQFSAALRLPKAMKTHEKNERINTIIKELGLDKVADSKVGTQFTRGISGGERKRTSIGMELITDPSILFLDEPTTGLDSSTANAVLLLLKRMSKQGRTIIFSIHQPRYSIFKLFDSLTLLASGKLMFHGPDSNAAILLRITYKNLRSKSGIKVFRFIAPLYYINKECFKSALYKKTLNPVLVKAAWKKAAKRKLKEETVTFHGDPDEVSMQLSHDPLELHTVVIDCSAIQFLDTAGIHTLKEVRRDYEAIGIQVLLAQCNPSVRDSLAKGEYCKKEEENLLFYSLSEAVAFEVTSSSTSSSTSPYPSSRTVPGILILEIDAPIYFANASYLRERIIRWIDEEEERVKQSGESSLQYIILDMSAVGNIDTSGISMMVEIKKVIDRRALKLVLSNPKGEVVKKLTRSKFIGDHLGKEWMFLTVGELNAILGPTGGGKSSLLDVLAARKDPHGLSGDVLINGAPRPANFKCNSGYVVQDDVVMGTLTVRENLQFSAALRLPTTMTNHEKNERINMVIQELGLDKVADSKVGTQFIRGVSGGERKRTSIAMELITDPSILFLDEPTG"
  },
  {
    "reference": "MEVQLQELVDKIKKDGVAAADEKAAEIIRAAEEKAKNIIEKAEAEAQESVKKAEAEALRFQKAAESSIDQAGRNTLISFRQGLLNELNAIIKAETAKNYDSAVLKNLIPEAVKGWVKTGNTENLSVILADKDLKELESSLSAALKDHIAKGMELKADSKIAGGFRIGTKDGSAYYDFSAEAVADLFSSYLSPKTAEILKNAAKEL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Produces ATP from ADP in the presence of a proton gradient across the membrane..2. A protein with ATP binding, proton-transporting ATP synthase activity, rotational mechanism is needed to enhance proton motive force-driven plasma membrane ATP synthesis in a specific context. The designed protein sequence is ",
    "response#1": "VNPLDRDREWTVSVKVKLGDRVKPGQVVAEVPETSIVTHRVMVPPHLSGEVVEVVPNGKYTVDQEIVTVKDDKGNNHQIRLHQKWPVI",
    "response#2": "GQRVFDTFFPLGMGGTAAIPGGFGAGKTMTQHQLAKWSSADIIVYVGCGERGNEMTDVLEEFPKLEDPSTGKSMMERTVLIANTSNMPVAAREASIYTGITIAEFYRDMGYNVALMADSTSRWAEALREISGRLEEMPAEEGFPAYLPSRLAEFYERAGYVKTLGQDREGSISLIGAVSPPGGDFSEPVTQNTKRYVRCFWALDKSLASARHFPAVNWLESYHGWLKENINEDWIKLRNMAMELLKEEDRLQEIVKLVGEDVLPDNQRLILEVARLIKIGFLQQNAFSKVDRFATPEKQYWMLKIIMFLYEKARPLIKNGQRVFDTFFPLGMGGTAAIPGGFGAGKTMTQHQLAKWSSADIIVYVGCGERGNEMTDVLEEFPKLEDPSTGKSMMERTVLIANTSNMPVAAREASIYTGITIAEFYRDMGYNVALMADSTSRWAEALREISGRLEEMPAEEGFPAYLPSRLAEFYERAGYVKTLGQDREGSISLIGAVSPPGGDFSEPVTQNTKRYVRCFWALDKSLASARHFPAVNWLESYH",
    "response#3": "GQRVFDTFFPLGMGGTAAIPGGFGAGKTMTQHQLAKWSSADIIVYVGCGERGNEMTDVLEEFPKLEDPSTGKSMMERTVLIANTSNMPVAAREASIYTGITIAEFYRDMGYNVALMADSTSRWAEALREISGRLEEMPAEEGFPAYLPSRLAEFYERAGYVKTLGQDREGSISLIGAVSPPGGDFSEPVTQNTKRYVRCFWALDKSLASARHFPAVNWLESYHAIPFLKKK"
  },
  {
    "reference": "MADPLHVIVLAAGEGKRMKSLLPKVLLPLAGRPLLAHVLDTARALGPAGLHVVYGHRGEQLRAAFARDTDIHWVHQPEPLGTGHAVRLALHGIPDAARVLVLYGDVPLLRAATLRELIATHAELAVLAAELDDPRGYPRVLRNGLGRVRAIVQDQDADADARAARVVDTGVVFAEARCLRRWVDRLAADHPQGELYLGDAFALAAADGTPADVALTGNRIEAFGAADAAQLAALTAYLRRRDATRLMLEGVRLADPARFDLRGHFSHGRDVEIDIDAIMEGGVTLGDAVKIGPFCRLRNVNLAAGTEVRAHCDLEGVTTYGACRIGPFARLRPGTELAAGAHIGNFVETKKTKLGEGSKANHLTYLGDARIGDGVNIGAGTITCNYDGVAKHITEIGDGAFIGSNTALVAPVTIGANATIGAGSTITRNAPAEQLTLARAEQATVSRWQRPTREED",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Mg(2+) is required for the proper folding and stability of the protein.2. The protein should be designed to localize specifically to cytoplasm in order to perform its role in UDP-N-acetylglucosamine biosynthetic process, cell wall organization, cell morphogenesis more effectively.3. The designed protein should be able to compete with endogenous proteins or inhibitors for UDP-N-acetyl-alpha-D-glucosamine, Mg(2+), acetyl-CoA binding.4. The protein have enhanced acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.5. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc); The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.. The designed protein sequence is ",
    "response#1": "VIVLAAGHGTRMKSTTSKLLHKVAGKSMLSYAVQAAKALSPQHLVVVVGHGREQVQAHLGEIAPEATIAVQEEQLGTGHAVRCGLGALPPQVRGTVVVTYADVPLLSGETLGELVRTHARQANAVTVLTAELDDPTGYGRIVRDHGRVLRIVEHKDATPAEREIGEVNSGIYVFDSELLREGLAQIGRDNSQRELYLTDVVEYANRIGRGVGAFQTTDAWQTMGEDRAVLAVNERSQLAEAATEMQLRLMNRRRAELARAEALFQRRSRAEAQENGVTMVDPETVYFALDTYLGRDVTVGPHVVFGPEVTVESGAAIEAFCHLEGCHISRGATIGPFARLRPGAELAEDVHVGNFVEVKNSILGEGVKVGHLTYLGDADVGEGTNIGAGTVTCNYDGVFKHRTTIGAHAFIGSDTMLVAPVTVGDGALTGSGSTITQDVPPGALALGRARQRNVEGWVARRRPGSAAARAAERALAARGEPS",
    "response#2": "VLVLAAGPGTRMRSDTPKVLHTIAGRSMLSHSLHAVAKVAPRHLVVVLGHDHQRIAPLVAGLAESMGRTIDVALQDRPLGTGHAALCGLSALPDDYAGIVVVTSGDTPLLDSDTLAGLIAAHTAAPAAVTVLTTTVTDPTGYGRIVRGPDGAVTGIVEHRDATDAQRAVTEINSGVFAFDAAVLRDALGRIGTDNDQGEEYLTDVLGILREAGHRVGASVAADHREIAGVNDRAQLAEARRVLNDRLLRAAMLAGVWMLDPGRVYIDVSVRLEAGVRILPGVHLEGSTSVAEGAELGPDCFIRDSRIGPGARIWYSVVRGVSVGTDAQVGPYASLRPGVELGDSAKAGTFVEVKNSVVGARAKVPHLAYVGDTDIGEEANVGAGTITANYDGYNKHRTRIGSRAQIGSNTVLVAPVEVGAEAYTGAGSAITRDVPDGALGVERTSQRNIAGWVVRRRPGSAAARAAEAATGTEAAGSETTGNETAGSGTAGSGTAGSGTAGTAGTATTDTEAK",
    "response#3": "AVVLGAGEGSRMKSILPKVIHPVAGRPMIEWVIDATRSLNPVQTVVVVKPDADEVVAMLPDGATPVVQPKQLGTAHALQHALEIIPLDADDHVLVVPADTPLITGDTLGEMAKLHRRTGAAVTCLTANMDDPTGYGRILRDDDGAVLGIVEEREATPEQREITEINSGVYVFAAPALRAGLARLTTSNAQGEEYLTDVVAILRGDGHRVGAHLAADRWLVEGVNDRVQLAQLGAELNRRTVERWQRASHLGDDVFIGSNSVLVAPVDIASGAFVAAGSTVVADTHIGRDAVIAPNVVFGPGVTVESGAEIKAFCHLRDGAVIEPGTVIGSDSYAAAYTRAGERGVTMRDPSTVYVDHGVKIATDVVLDPGVILNGSTMIGEGSVIGPDTTIVDSAIGRDCSVRRSVIEGSVVEDGASIGPYSHLRSGSHVGAGAEVGNYAEVKNSRLGARVKQHHMSYLGDAEIGEGTNVGAGTITANWDGGAKNRTRVGRNAFLGVDTMLVAPVDVGDGAMTGAGAVVTKDVPPGKLAVPRGWTVVGVPGRSDLPKVLHKIAHAPMLWHALRAGLVLEPEKTVVVAGHGADLVQASAHEFDPEIQIALQTEQLGTAHAVAQARPALDGFEGDAIVLYGDTPFIREETLAAITQARVSNDVVVLGS"
  },
  {
    "reference": "MGGKWSKFSGWSAVRDRMRRAEPAAVGVGPASRDLERHGALTTSNTATNNADCAWLEAQEDEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSTKRQEILDLWVYHTQGFFPDWHNYTPGPGVRYPLCFGWCFKLVPMEPEDVEKANEGENNCLLHPISQHGMDDTEKEVLVWRFDSRLAFHHVARELRPEYYKDC",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in membrane, virion component that is critical for suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II.2. The protein design should include a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Dileucine internalization motif; necessary for CD4 internalization, Diacidic; necessary for CD4 internalization motif to ensure proper function.3. The protein must exhibit the following characteristics: Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1. The designed protein sequence is ",
    "response#1": "MGGKWSKSSLVGWPAVRERMRRAET",
    "response#2": "MGGKWSKSSLVGWPQIRERMRRAEPAEPAAEGVGAASRDLERHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPEKVEEANEGENNCLLHPMSQHGMDDPEREVLMWKFDSRLAFHHMARELHPEYYKN",
    "response#3": "MGGKWSKSSIVGWPAVRERMRRTDPATAAEGVGAASQDLDKYGALTSSNTAHNNADCAWLEAQEDEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQRRQDILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPEKVEEANEGENICLLHPMSLHGMEDPEKEVLEWRFDSRLAFHHVAREKHPEFYKDC"
  },
  {
    "reference": "MLFFSYFKDLVGQEVTVELKNDLAIRGTLHSVDQYLNIKLENTRVVDQDKYPHMLSVRNCFIRGSVVRYVQLPKDGVDVDLLHDAARREARGG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design must be able to perform RNA binding in order to contribute to deadenylation-dependent decapping of nuclear-transcribed mRNA. The designed protein sequence is ",
    "response#1": "VNDIMPMIGARFYTQLDAAQMRNDVIEEDLAKEVQNGRLFRLLAKLGTINERPEFQKDPTWSETGDRYLLKLFRDHLFHQVTEAGAPWIDLSHIISCLNKLDAGVPEKISLISRDEKSVLVVTYSDLKRCFENTFQEC",
    "response#2": "VNDIMPMIGARFYTQLDAAQMRNDVIEEDLAKEVQNGRLFRLLAKLGTINERPEFQKDPTWSETGDRYLLKLFRDHLFHQVTEAGAPWIDLSHIISCLNKLDAGVPEKISLISRDEKSVLVVTYSDLKRCFENTFQEC",
    "response#3": "TQVNKSHCYGYVTSVYRATNVKTGLVYCLRRIHGFRLSSTKTIIEIIDHWKKIQHSNLVQLRELFTTKAFGDNSVVFVYDFFAGAQTMKQVYFNGLGASGAGNGSGASGAAGGYNTTPATHNGMGQQQAKNRVGGRGLQQHPDGSLRQGHGLLPESLIWTYVVQLSSALRTVHAANLAIRCLDPSKVLLSSAGKSRLRLNCAAIVDVITLDSAQAGGGAGNQRTAAMVHCYQKEDLAHLGRLILCLACNSSSAYQFVESSMELVTKNYSSDLRNLLVLLLSMPPRVSSINDVMPMIGARFR"
  },
  {
    "reference": "MQKLVIATGNAHKLHEIRVLLEPLGIAVLPQSDFNVPEAAEPHCTFIENALAKARHAARLTGLPALADDSGICVEALGGAPGVISAIYAGEPRSDARNNIKLLEAMRDVADRRAHYHCALVLLRHADDPQPIIAEGQWHGVILEAPRGEGGFGYDPLFLDTELNQTVAEISTADKNRVSHRGKALRALVEKLRNIQG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.2. The protein must bind to Mg(2+) in order to perform its enzymatic function.3. The protein design must be able to perform ITP diphosphatase activity, nucleotide binding, ribonucleoside triphosphate phosphatase activity, dITP diphosphatase activity, metal ion binding in order to contribute to nucleotide metabolic process, purine nucleoside triphosphate catabolic process.4. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+).5. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions. The designed protein sequence is ",
    "response#1": "MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAIKAGSELGLQAKTIMESGGLVSDELIINLIKDRIAQDDCKNGQGFILDGVPRTIPQAEALDKMLSEMGISLDYVIEIEVPENVIVERMVGRGLSEGTADKVYHNLETYKQPLLEYYSEKTKLVSVDGTGKIEEVTNRIIAAFEKLN",
    "response#2": "MKNYINEIKNHFIPFVKKLKNGEIIIVTDDEDRENEGDFIMAAEKVTPEIINFMAKYGRGLICMPITAERAQELQLKPMVDKNTDSFETAFTVSIDAKGTSTGISAFDRSMTVHKLCSPKTKKNDFIRPGHIFPLIAKDGGVLVRAGHTEASVDLARIAGCYPAGVICEIIKEDGTMARLSDLLEFTKKHNLKIITIADLIKYRFRNEKLIERGC",
    "response#3": "MYFNFDKIEQAIEDIKNGKMIIVVDDEDRENEGDLLMAAEKITPEAINFMAKYGRGLICVSMEGKRLDELEIPLMVNDNTSAMGTNFTVSIEAKEGVTTGISAHDRATTILTAIDPKSKPEDLRRPGHIFPLIAKEGGVLKRAGHTEAAVDLARLAGLYPAGVICEIIKEDGTMARVPELMEFAKKHNLKIITIKDLIAYRLKKESLIKKEICAFEIPTHKFGYLKIECLKKEIGHNLPEIKNKNYLYLKTKKEKLGHFLNKDEINYLEIKKDKLGHFLNLDELKIFYDSLKFLAFQCEKEKKPQEITLCFSDKPVNLFEKIKYILSIKNLEGLKFSDYKKDYLNILKKKINDLFNLEEINLEKF"
  },
  {
    "reference": "MSTEVNANQSPNAESRQEAARSGERDKALNLVLGQIERNFGKGSIMRLGDASRMRVETISTGALTLDLALGGGYPKGRVVEVYGPESSGKTTLTLHAIAEVQRRGGVAAFVDAEHALDPVYAASLGVDVENLLVSQPDTGEMALEIVDQLIRSAAVELVVVDSVAALTPRAEIEGEMGDHVIGSQARLMSQAMRKITGNIGKSGCTVIFLNQLRLKIGVTYGNPETTTGGNALKFYASVRLDIRRIQTLKRGTDEYGIRAKVKVAKNKVAPPFRIAEFDILFGKGISTIGCLLDLAEETNIVARKGAWYSYEGDNIGQGRDNTITWLEQNTEAKEKIEKLVRQKLTEGSEVSSNSMRPLTTANRKAA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: It interacts with LexA causing its activation and leading to its autocatalytic cleavage..2. The protein must be able to ATP binding. The designed protein sequence is ",
    "response#1": "EQSVEALAGHLVENDNIQVVSISGMGGIGKTTLARQVFHHDMVQRHFDGFAWVFVSQQFTQKHVWQRIWQELQPQNGDISHMDEHILQGKLFKLLETGRYLVVLDDVWKEEDWDRIKAVFPRKRGWKMLLTSRNEGVGIHADPKSFGFKTRILTPEESWKLCEKIVFHRRDETGTLSEVRVDEDMEAMGKEMVTCCGGLPLAVKVLGGLLATKHTVPEWKRVYDNIGPHLAGRSSLDDNLNSIYRVLSLSYED",
    "response#2": "AEGVVLFGVHKLWELLNRESARLNGIGEQVDGLKRQLGRLQSLLKDADAKKHESERVRNFLEDVRDIVYDAEDIIESFLLNEFRTKEKGIKKHARRLACFLVDRRKFASDIKGITKKISEVL",
    "response#3": "AEGVVLFGVHKLWELLNRESARLNGIGEQVDGLKRQLGRLQSLLKDADAKKHESERVRNFLEDVRDIVYDAEDIIESFLLNEFRTKEKGIKKHARRLACFLVDRRKFASDIKGITKKISEVL"
  },
  {
    "reference": "MRLLIVLGLLWSLVATLLGSKWPEPVFGRLVSLGFPEKYGNHQDRSWTLTAPPGFRLRLYFTHFNLELSYRCEYDFVKLTSGTKVLATLCGQESTDTERAPGNDTFYSLGPSLKVTFHSDYSNEKPFTGFEAFYAAEDVDECRTSLGDSVPCDHYCHNYLGGYYCSCRVGYILHQNKHTCSALCSGQVFTGRSGFLSSPEYPQPYPKLSSCAYNIRLEEGFSITLDFVESFDVEMHPEAQCPYDSLKIQTDKREYGPFCGKTLPPRIETDSNKVTITFTTDESGNHTGWKIHYTSTAQPCPDPTAPPNGHISPVQATYVLKDSFSVFCKTGFELLQGSVPLKSFTAVCQKDGSWDRPIPECSIIDCGPPDDLPNGHVDYITGPEVTTYKAVIQYSCEETFYTMSSNGKYVCEADGFWTSSKGEKSLPVCKPVCGLSTHTSGGRIIGGQPAKPGDFPWQVLLLGETTAAGALIHDDWVLTAAHAVYGKTEAMSSLDIRMGILKRLSLIYTQAWPEAVFIHEGYTHGAGFDNDIALIKLKNKVTINRNIMPICLPRKEAASLMKTDFVGTVAGWGLTQKGFLARNLMFVDIPIVDHQKCATAYTKQPYPGAKVTVNMLCAGLDRGGKDSCRGDSGGALVFLDNETQRWFVGGIVSWGSINCGGSEQYGVYTKVTNYIPWIENIINNF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Incorporate a signal peptide in the protein design.2. The designed protein should contain one or more Peptidase S1, CUB, Sushi, EGF-like; calcium-binding domains that are essential for its function.3. The engineered protein is expected to adopt a strand-turn-strand-strand-helix-strand-strand-strand-strand-strand-strand-turn-strand-strand-strand-based conformation.4. The design of the protein must focus on the precise arrangement of amino acids in the Charge relay system to enable effective catalysis of Selective cleavage after Arg-223 in complement component C2 (-   Ser-Leu-Gly-Arg-|-Lys-Ile-Gln-Ile) and after Arg-76 in complement   component C4 (-Gly-Leu-Gln-Arg-|-Ala-Leu-Glu-Ile)..5. For general function, the protein need meet that Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin; After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase.6. The ideal protein would be one that plays a critical role in complement activation, lectin pathway, positive regulation of opsonization, complement activation, cell surface pattern recognition receptor signaling pathway, proteolysis through serine-type endopeptidase activity, complement component C4b binding, peptidase activity within extracellular space, serine-type endopeptidase complex. The designed protein sequence is ",
    "response#1": "MSVNASDQERTPWQVTFKPKSKETCQGSLISDQWVLTAAHCFHDAQMEDRHLWRVIVGDPTSHHGKEFHVEEVLVAPGFNVHAKQSQGISEFYADDIALLKLSQRVKMSSHARPICLPCTVGANMALRRSPGSTCKDHETELLSQQKVPAHFVALNGNRMNINLRTGPERTSCIEAVSQNKGSFPGLTNVSEVVTDQFLCSGMEGDDSPCKGESGGAVFLERRYRFFQVGLVSWGLFDPCATKTSRYFKPELPYFIQVEVRNADGSPSKEVDVVAKVQVGSATINPQKMRTDSNGLTSFTVTPPNVNQLTVTVRTDERHPSNEQGELVAEFQDLPAATKTSRYFKPELPYFIQVEVRNADGSPSKEVDVVAKVQVGSATINPQKMRTDSNGLTSFTVTPPNVNQLTVTVRTDERHPSNEQGELVAEFQDLPALCLKPEIEYGRLSVEKVRYVEPEIITIQCESGYSVVGSENITCSEDRTWYPEVPKCEIGVGNMSANASAQERTPWHVTIKPKSQETCRGALISDQWVLTAAHCFRNAEDRTLWRVSVGDPNFQGSKEFQIEEAVISPGFNVFSKKSQGIPEFYGDDIALLKLTQKVKMSTHARPICLPCTVGANLALRKLPGSTCRDHEKELLNQVSIPAHFVALNGDKLNINLKTGSEWTNCVKVVLKDKTTFPNLTDVREVVTDQFLCSGTQGDDSPCKGESGGAVFLERRLRFFQVGLVSWGLYNPCGGSSKNSRKPAPHGKVPRDFHINLFRLQPWLRQHL",
    "response#2": "MSVNASDQERTPWQVTFKPKSKETCQGSLISDQWVLTAAHCFHDAQMEDRHLWRVIVGDPTSHHGKEFHVEEVLVAPGFNVHAKQSQGISEFYADDIALLKLSQRVKMSSHARPICLPCTVGANMALRRSPGSTCKDHETELLSQQKVPAHFVALNGNRMNINLRTGPERTSCIEAVSQNKGSFPGLTNVSEVVTDQFLCSGMEGDDSPCKGESGGAVFLERRYRFFQVGLVSWGLFDPCSRPPSYTEQNIYLVLDGSDSIGSSNFTGAKRCLTNLIEKVASYGVRPRYGLLTYATVPKVLVRVSDERSSDADWVTEKLNQISYEDHKLKSGTNTKRALQAVYSMMSWAGDAPPEGWNRTRHVIIIMTDGLHNMGGNPVTVIQDIRALLDIGRDPKNPREDYLDVYVFGVGPLVDSVNINALASKKDNEHHVFKVKDMEDLENVFSDQERTPWQVTFKPKSKETCQGSLISDQWVLTAAHCFHDAQMEDRHLWRVIVGDPTSHHGKEFHVEEVLVAPGFNVHAKQSQGISEFYADDIALLKLSQRVKMSSHARPICLPCTVGANMALRRSPGSTCKDHETELLSQQKVPAHFVALNGNRMNINLRTGPERTSCIEAVSQNKGSFPGLTNVSEVVTDQFLCSGMEGDDSPCKGESGGAVFLERRYRFFQVGLVSWGLFDPCHSQPPSYTEQVSLIYGRTKTEGLVQVKLVDQDERMFICKNSWSMAEANVACVDLGFPLGVRDIQGSFNISGNLHINDTECLHVHCRGVETSLAECAFTKRRTELSNGLAGVVC",
    "response#3": "LCGMVWEHKKGNDYHKQPWQAKISVTRPLKGHETCMGAVVSEYFVLTAAHCFMVDDQKHSIKVSVGGQRRDLEIEEVLFHPKYNINGKKAEGIPEFYDYDVALVKLKNKLKYGQTLRPICLPCTEGTTRALRLPQTATCKQHKEQLLPVKDVKALFVSEQGKSLTRKEVYIKNGDKKASCERDATKAQGYEKVKDASEVVTPRFLCTGGVDPYADPNTCKGDSGGPLIVHKRSRFIQVGVISWGVVDVCRDQRRQQLVPSYARDFHINLFQVLPWLKDKLLSS"
  },
  {
    "reference": "MSTTAMSVVILAAGKGTRMYSDLPKVLHTLAGKPMVQHVIDAATGLGAQQIHLVYGHGGDQLKAQLAGNTLNWVLQAQQLGTGHAMQQAAPYFADNEDILMLYGDVPLISQETLQRLRAAKPAGGIGLLTVVLDNPTGYGRIVREHDTITGIVEQKDASAEQLKIQEINTGILVANGGDLKRWLSQLNSNNAQGEFYITDIIAMAHQEGRVVQAVHPARISETDGVNNRLQLAALERAYQAEQAERLLLAGVMLRDPARFDLRGTLQHGRDVEIDTNVIIEGHVTLGDRVKIAAGCIIKNSVIADDCEISAYSVIEDASLAAACTVGPFARLRPGSELGEKAHVGNFVEMKKASLGKGSKAGHLSYLGDAEIGEDVNIGAGTITCNYDGANKSKTIIGDNVFVGSDTQLVAPVTIASGATIAAGTTVMKDVPAAVLVYNRKEQNQKAGWQRPVKKK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be optimized for UDP-N-acetylglucosamine diphosphorylase activity, magnesium ion binding activity in order to improve lipid A biosynthetic process. The designed protein sequence is ",
    "response#1": "PKVLHTIGGRSLLGHAVAAAEGLQPAHLAVVVGHQRERVGEHVAQIAPEAVVAHQDDVPGTGRAVQCGLLALPELSGTVVVTYGDVPLLSTDTLQRLVDEHEESASAVTVLTAHVDDPAGYGRVIRDADGQVARIVEQKDADESERAVREINSGIYAFDAGVLREALGQVTTDNAQGEMYLTDVLAIARAAGRRVSALPLDDVWRQVTSDAELARRTAAELDRRTAAGR",
    "response#2": "PKVLHTIGGRSLLGHAVAAAEGLQPAHLAVVVGHQRERVGEHVAQIAPEAVVAHQDDVPGTGRAVQCGLLALPELSGTVVVTYGDVPLLSTDTLQRLVDEHEESASAVTVLTAHVDDPAGYGRVIRDADGQVARIVEQKDADESERAVREINSGIYAFDAGVLREALGQVTTDNAQGEMYLTDVLAIARAAGRRVSALPLDDVWEQLGVDDPAELAAARRRL",
    "response#3": "PKVLHTIGGRSLLGHAVAAAEGLQPAHLAVVVGHQRERVGEHVAQIAPEAVVAHQDDVPGTGRAVQCGLLALPELSGTVVVTYGDVPLLSTDTLQRLVDEHEESASAVTVLTAHVDDPAGYGRVIRDADGQVARIVEQKDADESERAVREINSGIYAFDAGVLREALGQVTTDNAQGEMYLTDVLAIARAAGRRVSALPLDDVWSELGVESAERRVRELVEAGL"
  },
  {
    "reference": "MLKNKTVVLGVTGGIAVYKALDLISRLRKRDITIHVIMTKSATQFVSPLSFQSLSQNMVISDMFAEPKAWEIQHISLAQKADLLAVVPATANIIGKIANGIADDMLSTTIMATNAPVVFAPAMNCNMYNNSIVQENITKLKKHGYDFIEPVKGRLACGDEGIGKLADTSLIEEAICSKLYDVKDLKDKKVIVTAGPTIERIDPVRIITNNSSGKMGYAIAEEARDRGAHVVLISGPTNLKPPYGVEYISVNTNEEMYNEVMKYYEEADIVVKNAAVADYRPRAYSDKKIKKGSEEFTIELVKNRDILYTLGQNKKNQVLVGFAAESHDVIENAEKKIKKKNLDYIVANCILNSDTGFKSEYNKATILSKDGLKVQTERITKRELASELFNLINKKR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The CTP binding site should be stable and able to withstand changes in environmental conditions.2. The protein must have high specificity towards FMN binding, metal ion binding, phosphopantothenoylcysteine decarboxylase activity.3. A protein should be able to manipulate the activity of enzymes in the coenzyme A biosynthesis pathway, either by activating or inhibiting their function.4. The designed protein is the optimization of the Proton donor to achieve high catalytic efficiency for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.5. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A6. The designed protein should contain one or more DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MRVLFGVGGGIAAYKAVQAVRLLVRAGHDVHVVPTENALRFVGAPTWEAISRNPVDRDLFTDVAGVRHVRMGAAADLVVIAPATADLIARMAQGRADDLLTASLLVAECPVLIAPAMHSQMWRHPATQANVATLHDRGVQVLGPGIGALTGPDEGVGRMVEPEDIAVELAALLGDPAALPRLAGRLVVISAGGTRERIDPVRYLGNDSSGKQGMALAEEALARGAEVVVVAGHLDVPAPRGAEVISARYTESMRQAITSLADEADVVIMAAAIADYRPANQTTTKIKKIDEGDETTLRLVSNPDILSEISHRPDRGNVVVGFAAETASGAALLELGRAKIRRKGCDLLAVNEVGEEKGFGRDENTVSVLGADGSETPVPHGSKLLLAHAIWDAVRRLR",
    "response#2": "MRIILGVGGGIAAYKSAFLLRLFKEAGHDVTVIPTEAALSFVGQATWEALSGHPVSTTVFDAVDTVNHVRLGQEADLVVVAPATADLLAQAAAGLAGDLLTNTLLATRGKVVFVPAMHTEMWQHPATQANVALLRSRGTAVMEPASGRLTGKDTGPGRLPDPEDIYAFALDLQGLVDTVLAARGLAGERILVTAGPTREEIDPVRFISNHSSGKMGYAIARQARLRGAEVTLVTGPTAITPPLGVAVIRVESAEEMREAVLARLEETDIVIKAAAVADYRPRLRAQMKVKKTAAHLAIELEKNPDILAEIGTRKEGRVLVGFAAETEDLVANATLKLTLKNLDLVVANDVSQEGAGFHVDTNIAKVLWPDGGEELAGRHVLVTSGPTHEPIDPVRYIANRSSGAQGTAIAAALRDLGARVTFVTGPASVPPPPGVDVVAVGTAAQMAAAVRAALPADAAVMAAAVADWRIANPGTQKMKKDGSDRPPALDFAENEDILAAVSRGEPRPRLVVGFAAETEKVVEHATAKRLRKGCDWILANDVSPETGIMGGAENAVTLLFHEGAKTALLAGRLVVISAGGTRERIDPVRYLGNDSSGKQGMALAEEALARGAEVVVVAGHLDVPAPRGAEVISARYTESMRQAITSLADEADVVIMAAAIADYRPANQTTTKIKKIDEGDETTLRLVSNPDILSEISHRPDRGNVVVGFAAETASGAALLELGRAKIRRKGCDLLAVNEVGEEKGFGRDENTVSVFWPGGEKEVPR",
    "response#3": "MRIILGVGGGIAAYKSAFLLRLFKEAGHDVTVIPTEAALSFVGQATWEALSGHPVSTTVFDAVDTVNHVRLGQEADLVVVAPATADLLAQAAAGLAGDLLTNTLLATRGKVVFVPAMHTEMWQHPATQANVALLRSRGTAVMEPASGRLTGKDTGPGRLPDPEDIYAFALDEILAFLRPKVLAGERILVTAGPTREEIDPVRFISNHSSGKMGYAIARQARLRGAEVTLVTGPTAITPPLGVAVIRVESAEEMREAVLARLEETDIVIKAAAVADYRPRLRAQMKVKKTAAHLAIELEKNPDILAEIGTRKEGRVLVGFAAETEDLVANATLKLTLKNLDLVVANDVSQEGAGFHVDTNIAKRVFWEGGRVDEWPLMHKDQVAQRLWDWVIGLCKG"
  },
  {
    "reference": "MKWKKSLTGLSSYKPGKREEEVMAELGLTKITKLSSNENPLGTSPKVAELQADSSVETEIYPDGWASSLRTVVADFYQLEEEELIFTAGVDELIELLTRVLLDTTKNTVMATPTFVQYRQNALIEGAEVREIPLLVDGAHDLDGMLNAIDDNTTIVWVCNPNNPTGNYIDLADIQAFLDKVPSDVLVVLDEAYIEYVTPQPEKHEKLIRTYKNLIITRTFSKIYGLASARVGYGIADKAIIEQLNIVRPPFNTTSIGQKLAIEAIKDQAFIEACRTSNANGIKQYEAFAKRFEQVKLYPANGNFVLIDLGIEAGTIFSYLEKNGYITRSGAALGFPTAVRITIGKEEENSAVIALLEKLL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The presence of pyridoxal 5'-phosphate is crucial for the protein to function optimally under specific conditions.2. A protein that is capable of catalyzing reactions within the L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9 pathway with high efficiency and specificity.3. The protein must exhibit pyridoxal phosphate binding. The designed protein sequence is ",
    "response#1": "DPLKLSSNENPFGPSPAAVQAMADAVASSHRYPNTDHADLRAAISEIHGLEADNIICGVGSDEVIHFLCQCYAGPGDEVLYTQHGFGMYPIAAKAAGATPVQVAEDARHVDVDALIAGITPATRLIFIANPSNPCATMIDNGEITRLADALPDQCLLILDGAYVEFADGYDGGKNLVEARDNVVMTRTFSKVYGLGGLRVGYGYGPRHVIDTLNRIRGPFNLSGMALAGAEAAVRDVDWVNECLRVNADERARLVGGLRQLGLACDDSHANFVLARFASEAAADAADAHLKRDGIIVRAPKSYGLPDCLRITVGRPEDNSRVLG",
    "response#2": "DPLKLSSNENPFGPSPAAVQAMADAVASSHRYPNTDHADLRAAISEIHGLEADNIICGVGSDEVIHFLCQCYAGPGDEVLYTQHGFGMYPIAAKAAGATPVQVAEDARHVDVDALIAGITPATRLIFIANPSNPCATMIDNGEITRLADALPDQCLLILDGAYVEFADGYDGGKNLVEARDNVVMTRTFSKVYGLGGLRVGYGYGPRHVIDTLNRIRGPFNLSGMALAGAEAAVRDVDWVNECLRVNADERARLVGGLRQLGLACDDSHANFVLARFASEAAADAADAHLKRDGIIVRAPKSYGLPDCLRITVGRPEDNSRVLR",
    "response#3": "DLVKLNTNESPYGPSEKALGAIRAAADTDLRLYPDPVALGLRQAIAARHQVSVGEVFVGNGSDEVLAHTFAALLKHDRPLLFPDISYSFYTTYAGLFGIEAVEVPLDAAFRIDIADYRRPSGAVILPNPNAPTGIGLPLAEIERLVADHPGQPVVIDEAYIDFGGESAIALVPKYDNLLVVQTFSKSRALAGLRVGFAIGQRPLIEALERVKDSFNSYPLGRAAQAGATAAIEDEAWFEATRGKIIASRAKLTSELEKRGFEVLPSQANFVFARHPGHAGQTLAAKLRERAVIVRHFAKPRIADFLRITIGTDAECAKLVAAR"
  },
  {
    "reference": "MTRSANSPFEVAIVGGGITGLALAVGLLKRNVSFTIYERAENFGELGVGITFTPNAQRAMEALDPCVLQSFTNVASAPSGGTINFVDGVREQGSEDPRTSTAALLFQLHVKGGYKACRRCDFVDQIVQHIPKDCVQYRKWLDSIETDHESGRAVLKFRDGEIAHADVVIGCDGIRSQVRASMFGTDELCPRAQYSHQLGYRGMVPLAQATAVLGPEKTSSAVLHTGPGAFVLTIPLAEVHAMHIEAFIMDKEEWPEVQTSSDSKRYVLPATRNEATKAFAEFGPTVRSAVSMFPEKLEKWAVFDMLEAPVPTFAKGRVCLAGDAAHASTPNQGGGAGFGIEDALVLAEVLAVLAEAPNVSGIVASEALAVYSEVRYERSQWLVRSSRRTGELCTWKDRDWGLAAEELSRDIISRSHQLWDHDTAGMVSDALAILGERVRGADTAF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that SorB releases an aldehyde, which undergoes spontaneous cyclization resulting in the formation of sorbicillin or 2',3'-dihydrosorbicillin2. The designed protein must be able to perform monooxygenase activity under specific conditions.3. The designed protein should have a binding site that is specific to the target FAD ligand. The designed protein sequence is ",
    "response#1": "MRRFPATVTVITACATGDQRDHGMTVTAVTSVSMEPPSLLVCLNNRTFLHELLLCRPDFIVNVLTQDQIALSDAFSGKVSPEERFRNGEWQRHDNGVLYLPTAHAAIACRRVAAMPYGTHTVFIGQVVSADVSETTRPLLYENAQYCAR",
    "response#2": "MRRFPATVTVITACATGDQRDHGMTVTAVTSVSMEPPSLLVCLNNRTFLHELLLCRPDFIVNVLTQDQIALSDAFSGKVSPEERFRNGEWQRHDNGVLYLPTAHAAIACRRVAAMPYGTHTVFIGQVVSADVSETTRPLLYENAQYCG",
    "response#3": "MRRFPATVTVITACATGDQRDHGMTVTAVTSVSMEPPSLLVCLNNRTFLHELLLCRPDFIVNVLTQDQIALSDAFSGKVSPEERFRNGEWQRHDNGVLYLPTAHAAIACRRVAAMPYGTHTVFIGQVVSADVSETTRPLLYENAQYCAR"
  },
  {
    "reference": "MPRSFLVKKVKLDAFSSADLESAYGRARSDLGAPLHDKGYLSDYVGPSSVYDGDAEAALLKGPSPEPMYAAAVRGELGPAAAGSAPPPTPRPELATAAGGYINGDAAVSEGYAADAFFITDGRSRRKASNAGAAAAPSTASAAAPDGDAGGGGGAGGRSLGSGPGGRGGTRAGAGTEARAGPGAAGAGGRHACGECGKTYATSSNLSRHKQTHRSLDSQLARRCPTCGKVYVSMPAMAMHLLTHDLRHKCGVCGKAFSRPWLLQGHMRSHTGEKPFGCAHCGKAFADRSNLRAHMQTHSAFKHFQCKRCKKSFALKSYLNKHYESACFKGGAGGPAAPAPPQLSPVQA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein with DNA-binding transcription factor activity, sequence-specific DNA binding, RNA polymerase II transcription regulatory region sequence-specific DNA binding, DNA-binding transcription repressor activity, RNA polymerase II-specific functions is required to enable regulation of DNA-templated transcription, regulation of neuron migration.2. The protein must exhibit the following characteristics: Can modulate the action of basic helix-loop-helix (bHLH) transcription factors, critical for neuronal differentiation.. The designed protein sequence is ",
    "response#1": "GKRPDLLTFVQNLCKGLSQPTTNLVAGCLQLNARSFLMGQGGEAAHHTRSPYSTFYPPYHSPELTTPPGHGTLDNSKSMKPYNYCSAYESFYESTSPECASPQFEGPLSPPPINYNGIFG",
    "response#2": "GKRPDLVSYVQTLCKGLSQPTTNLVAGCLQLNSRNFLTEQGADGAGRFHGSGGPFAMHPYPYPCSRLAGDQCQAAGGLGGGAAHALRTHGYCAAYETLYAAAGGGGASPDYNSSEYEGPLSPPLCLNGNFC",
    "response#3": "GKRPDLVSYVQTLCKGLSQPTTNLVAGCLQLNSRNFLTEQGADGAGRFHGSGGPFAMHPYPYPCSRLAGDQCQAAGGLGGGAAHALRTHGYCAAYETLYAAAGGGGASPDYNSSEYEGPLSPPLCLNGNFC"
  },
  {
    "reference": "MKGTLTRAALAAGGMMVTSAVMAGSLALPTAQSLAGQWEVADSERQCQIEFLANEQSETNGYQLVDRQRCLQSVFAAEVVAGAGPDGIALLQADGSTLAFFSRDGDLYRNQLGAGDALTLKALA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must contain a signal peptide for proper functionality. The designed protein sequence is ",
    "response#1": "RGFGGAMTDAAAINILSLSAGAQEQLLQQYFSSEGIGYSVVRVPMASCDFSTRLYTYADTPGDYNLVNFTLAPEDVDMKIPLLKRAQAMSPQPLHLLASAWSAPAWMKTNGALVGKGSLKGQPGGKEHKAWAQYYIRFLEEYAKYNLTFWALTTGNEPSAGLITNYSFQALGFTPEEQRDWVALDLGPAVRSSSFPQTHILILDDNRLLLPYWATVVLSDVHAARYIHGVAVHWYLDSFVPAEISLGSTHHLYPEYYLFGTEACAGWSTGDRGVKLGSWERAEQYAHDIIQDLNQYVVGWTDWNLALDQAGGPNWVKNFVDSPVVVDAQRDVFYKQPTFYSLAHFSKFL",
    "response#2": "RGFGGAMTDAAAINILSLSAGAQEQLLQQYFSSEGIGYSVVRVPMASCDFSTRLYTYADTPGDYNLVNFTLAPEDVDMKIPLLKRAQAMSPQPLHLLASAWSAPAWMKTNGALVGKGSLKGQPGGKEHKAWAQYYIRFLEEYAKYNLTFWALTTGNEPSAGLITNYSFQALGFTPEEQRDWVALDLGPAVRSSSFPQTHILILDDNRLLLPYWATVVLSDVHAARYIHGVAVHWYLDSFVPAEISLGSTHHLYPEYYLFGTEACAGWSTGDRGVKLGSWERAEQYAHDIIQDLNQYVVGWTDWNLALDQAGGPNWVKNFVDSPVVVDAQRDVFYKQPTFYSLAHFSKFL",
    "response#3": "RGFGGAMTDAAAINILSLSAGAQEQLLQQYFSSEGIGYSVVRVPMASCDFSTRLYTYADTPGDYNLVNFTLAPEDVDMKIPLLKRAQAMSPQPLHLLASAWSAPAWMKTNGALVGKGSLKGQPGGKEHKAWAQYYIRFLEEYAKYNLTFWALTTGNEPSAGLITNYSFQALGFTPEEQRDWVALDLGPAVRSSSFPQTHILILDDNRLLLPYWATVVLSDVHAARYIHGVAVHWYLDSFVPAEISLGSTHHLYPEYYLFGTEACAGWSTGDRGVKLGSWERAEQYAHDIIQDLNQYVVGWTDWNLALDQAGGPNWVKNFVDSPVVVDAQRDVFYKQPTFYSLAHFSKFIPEAW"
  },
  {
    "reference": "MSDELLTPGQMAEADRLAIEGGPLDGYGLMLNAGRAVAAAVLARFPQARHAHILCGPGNNGGDGLVAARFLRAAGMAVSAYLGPPPREGGDAARALADFCDTVRPLEAFCATSDDVVVDALFGAGLARPLDGIFAALVDRVRQSEAPVVAVDLPSGVSGQSGAVLGAAVAARHTVTFFRRKPGHLLFPGRGLCGTVELVDIGIPRRVLAEIGVDLFENTPRLWLDALPVPDSQRHKYRRGHAGVFSGGPSATGAARLAAMAAARSGAGAVTLLSPASALQVNAAHLTSIMLRRADDPDDVAAFIAARKLSALAIGPAFGVGEKCRVFVAMAAGLRRESGLGLVLDADALTSFEDEPETLFAGLHTDGGGQAGPVVLTPHEGEFARLFPDLGADGGLSKVERARRAAARAGATVVLKGADSVIAARGGRAAISVHASPYLATAGSGDVLTGLIAGLLAQGVPGFEAAAAAVWLHGDAGLRLGAGLVAEDLPGTIPAVLAGLSAADDSVS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.2. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.3. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.4. The protein must be able to ADP-dependent NAD(P)H-hydrate dehydratase activity. The designed protein sequence is ",
    "response#1": "MRQVDRFAIEDIGMREEVLMENAGRAWVEQLEKLYAGLCKKKIAVLVGKGNNGGDGFVIARTLLDRGFHVDVWLLAAEEQWKGAAKYHKRLFESCGYEGKNWDVLAFMEKAYDLCVDAMLGTGATGALRYPYSEAADCLNKSGSIITAVDIPSGVPAGEGDVAEEAVQADATITLQSAKCSAYLFPARSYYGDITAVDIGIPQSAWEAVSRWLPVRRPGAHKGNCGHVVVIGGAPGYSGAGRLAGMAALRAGAGRVTLVTHPEHAALANLERPELMVRPAESARDLQTLLAGADAVAVGPGLGQQAWGRALWPAVADWTGPLVVDADALNLLAQAPRRRDDWVLTPHPGEAARLLAVTTGEVTADRVAAVDAIRRRFGGVVLLKGAGTLVAPAPGGGMACITGGHPGMASAGMGDVLTGVMAALRAQGLGPGEAAAAGAAWHVAAARLAARRIGAERGMLAGDVIDALPA",
    "response#2": "MRSADVFTIKEQEISSIELMETASAAFVKEFVREITDENTPIAVLCGKGNNGGDGLAIARILNDKGYNLVSVYLIDFSAQETEEYKVNLNRLKDQWFPLTTVKSVADLKNLKSGVIIDAVLGAGLNKKLSGLYLELAQFVNGLNLHVIAVDVPTGFPAEGKLDKDNVYIKAELVICFQRPKINFFFPESAAALNRFIVTPIGLSEEFIRDPDSYKGDNGEVLVVGGGPYAGAPTLSALAALRAGADLVRVACPGSVASEVQGFSPNLIVRPLPGDRLGPSHAERIASLAAGSDAVVLGPGLGDGDGTEKFVREFLTGYGGRAVVDADALGVVPEVDTDADLICTPHRGELVNMGGATVADPDERAELVREFAAELGHALLVKGSVDVISDGDEIRLNRTGNPGMTVGGTGDVLAGTVGALAAVTDPFRAAAVGAYAVGRAGDAAAGANGTGLVATDLPDRLPEAMALVIAGSPGKWGAGRMAARAALRAGAGLVTLAGPRADAAAPDEVMTAAIDPEAAGFDVALAELLAGKRAVAIGPGMPATAAGAALVRAVLERAEVPVVIDADALNHMAGDLAAVAAARAPVVLTPHPGEAGRLLGCSAADIERDRIAAVRRLAERTRAVVVLKGARTLVCDGAVDGFTTVNPTGNAAMATAGAGDVLTGVIVALAAQGAPPADAARAGVFLHGLAGDRAARGRIDAGLMAGDLIDELL",
    "response#3": "MRSADVFTIKEQEISSIELMETASAAFVKEFVREITDENTPIAVLCGKGNNGGDGLAIARILNDKGYNLVSVYLIDFSAQETEEYKVNLNRLKDQWFPLTTVKSVADLKNLKSGVIIDAVLGAGLNKKLSGLYLELAQFVNGLNLHVIAVDVPTGFPAEGKLDKDNVYIKAELVICFQRPKINFFFPESAAALNRFIVTPIGLSEEFIHPDSYKGDNGEVLVVGGGPYAGAPTLSALAALRAGADLVRVACPGSVASEVQGFSPNLIVRPLPGDRLGPSHAERIASLAAGSDAVVLGPGLGDGDGTEKFVREFLTGYGGRAVVDADALGVVPEVDTDADLICTPHRGELVNMGGATVADPDERAELVREFAAELGHALLVKGSVDVISDGDEIRLNRTGNPGMTVGGTGDVLAGTVGALAEAVSRWLPVRRPGAHKGNCGHVVVIGGAPGYSGAGRLAGMAALRAGAGRVTLVTHPEHAALANLERPELMVRPAESARDLQTLLAGADAVAVGPGLGQQAWGRALWPAVADWTGPLVVDADALNLLAQAPRRRDDWVLTPHPGEAARLLAVTTGEVTADRVAAVDAIRRRFGGVVLLKGAGTLVAPAPGGGMACITGGHPGMASAGMGDVLTGVMAALRAQGLGPGEAAAAGAAWHVAAARLAARRIGAERGMLAGDVIDALPA"
  },
  {
    "reference": "MHKVTSEELFQRALKVAPGGVHSPVRSFKGLDRAPVFFKKAEGAYLTSVEDKKYIDFCQSFGPLILGHLDAEVKEEVHRMVDTAWTFGATEIYSLELAEWITSTLPFMEKLRFVSSGTEAVMSALRVARAATGRTKILKFEGCYHGHVDNLLVKAGSGLAGTAASSSAGIPAEVAAHTVVAPLDDEAKLAEVFAAQGKDIAAVIIEPLPANYGLLVQRPEFLKKVAELCEKNGSLLIFDEVISGFRVGLGGMVEKTGIRPDLVTYGKIIGGGFPVGCYGGKAELMNMVAPSGDVYQAGTLSANPIGMRAGLTTLKKMQRVDGWNVLEQRTKKFTDAIKAGFVKKGVNLEISQVASLFWIHGPTASPIRSIDHIPANQGGNFKNLFLKALDKGVYLAPNAYEVGFVSLAHSDELLEQAAQIIVDAAE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should take into account the physiological conditions of the protoporphyrin-IX biosynthesis, 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2 pathway.2. The protein must bind to pyridoxal 5'-phosphate in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "METR",
    "response#2": "LIMDEVITGFRLALGGAQEYYRVKADITTLGKIVGGP",
    "response#3": "LIFDEVITGFRVSINGAQGYYNVFPDITTLGKIIGGQ"
  },
  {
    "reference": "MNILKDILYRVSMEAVTGNTSVAVNNIQFDSRKVELNDVFVAIRGTLSDGHKFIEQAVNKGALAVICEELPKDIVNGVTYVQVNDSQRALAYMAANFYDNPSEKLKLVGVTGTNGKTTIATLLYQLFTKAGFKVGLLSTVKVMVGDKSFDAVRTTPDSLTINSYLHQMNDEGVEFCFMEVSSHGIAQHRTSALEFKGGIFTNLSHDHLDYHKDFAEYRDVKKRFFDELPASAFALTNVDDKNGLVMLQNTKAVKYTYALKSYADYKAQILENQLTGLLLKVNGQELWTKLIGNFNAYNVLAIYATAELLGLETLETLKIISELDSVSGRFQYLISDKEKITAIVDYAHTPDALKNVLETINSIRTRNEKLITVLGCGGDRDKTKRPKMGHIASALSTDVIFTSDNPRTEDPDKIIEEIEAGVEPQNFKKTNAVTNRKQAIKTACQMAAKNDIILIAGKGHETYQEINGERFDFDDLKIVAEFLKQLEK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be designed to have high stability and activity towards ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.2. The protein design must feature the Meso-diaminopimelate recognition motif motif.3. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "MIITGTAGKTTTSYMVEAALLHHGISTGLIGTTGTRINGEKVPSTLTTPEAPNLQALFAMMKKRGVTHVVMEVSSHAIALGRVLGTHFSVAGFTNLSQDHLDFHPTMEDYFQTKARLFKEDSPLHAETAVVCIDDPWGQRLATMLPHPFTVSTEAASEHDASLQPDVWAGPSSVMDNGVQTAELYGFGANGMTLHIPMPGRFNVANAMLAMGALLGLDYPLSVLQQTLSHFTGVEGRMECAAVYRGAYVYIDYAHNPVSLENVLKTLRSYFGGKIFCVFGCGGNRAKSRRIGMGMVSGKMADLTIVTNDNPRFEDPEAIMDDIEDGLNAAGAEYIRIPDRKKAIRYGIVHAVAGDVVLLAGKGHENYQEVNGVKRPFSDLAEAERALASWQGGNA",
    "response#2": "ITGTSGKTTTTYLVESGLRAAGRTAGLIGTIGIRIDGIDVPSALTTPEAPALQAMFAAMAERGVDTVVMEVSSHALALGRVDGTGFAVGGFTNLSRDHLDFHPTMADYFEAKAKLFDPASPLRARRVVVCVDDDAGRAMAARAGDAITVSAADQPAHWRAMDVIPVGAGGQQFIAVDPAGVRHRVGIRLPGHYNVANCLVALALLGAMGFSLDDCISALSRFSGVPGRMEEIDCGQDFLALVDYAHKPEALRAVLATLRRPGRRLAVVFGAGGDRDPGKRRQMGEIAAELADLIVVTDDNPRREDPAAIRGEILAGARGGSAEVVEMGDRRAAIRHAAAWARPGDAVLIAGKGHETYQIIGSERMPFDDRQVARDALALRVS",
    "response#3": "MRLVTGTNGKTTVTYLLETIFQLAKQRTGVIGTIHTKIGDETYPVVNTTPDALFLQQTFHKMVQQNVDKAIMEVSSHALVQGRVHGCNYDIAVFTNLSQDHLDFHKDMDDYLRAKSLLFAQMGNNYNHTKHKYAIINQDEAASEFLKQSTAQQVLTYGIHQASQVMAQDIELGAAGTHFHLITPVGNIRIESKLMGMFNVYNMLAASATTALELGMPFDAIDEGLKRFAGVKGRMEMLHLKNGAVAVVDYAHKPNALKQALLSLRDHLEEGSLSVVFGCGGNRDKGKRPLMAAIAEAHADKIIITSDNPRFEEPMAIIEDIQAGLSDPTSENVLIEADRAAAIAIAIERSAPGDIILIAGKGHEDYQILGTEKIHFDDREEAAAALEKLRAR"
  },
  {
    "reference": "MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGHQADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGEISAVMGTVGNGLLGKVIPTENTTGSAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKATEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that can perform ATP binding, magnesium ion binding in cytosol to support regulation of cell shape, peptidoglycan biosynthetic process, cell division, cell cycle.2. The protein design should prioritize the formation of helix-turn-strand-turn-strand-helix-helix-strand-turn-strand-strand-helix-helix-strand-helix elements.3. The protein must exhibit the following characteristics: Is also able to use many meso-diaminopimelate analogs as substrates, although much less efficiently, but not L-lysine.; Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.4. The UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate binding site should be stable and able to withstand changes in environmental conditions.5. We require a protein that can specifically bind to the metabolites produced in the Cell wall biogenesis, peptidoglycan biosynthesis pathway.6. The protein must bind to Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MLKYTGVAIDSRKVKPGDLFIAYPGEAADGRNFIAQAIAAGAVIVVWEASGFEWHPDWKVPNVPVAGLRYHAGHIAEHLADIAIFTNLSQDHLEYHHTMEKYAEAKSLLFSQLGNGYQQGKAKFAILNFDDPVGKSFEEKTAARVFSYGIDQPADFKATDLSITNKGTRFNLNFENRVYEVQLQLVGKFNVSNALAAIAGAYAAGLEVSSVIQSIGEISGVRGRFELVKGDQDFAVIVDYAHTPDGLLNVLNAVNEIKTGNVFCVVGCGGDRDRTKRPIMAEIAQKNSDTVIFTADNPRTEDPIAILDEMVEGLRRLGYDIAIFTNLSQDHLEYHHTMEKYAEAKSLLFSQLGNGYQQGKAKFAILNFDDPVGKSFEEKTAARVFSYGIDQPADFKATDLSITNKGTRFNLNFENRVYEVQLQLVGKFNVSNALAAIAGAYAAGLEVSSVIQSIGEISGVRGRFELVKGDQDFAVIVDYAHTPDGLLNVLNAVNEIKTGNVFCVVGCGGDRDRTKRPIMAEIAQKNSDTVIFTADNPRTEDPIAILDEMVEGFGRAIN",
    "response#2": "MRVTGVTLDSRAVRPGDLYAALPGSRAHGADFAAQVADAGAAAVLTDADGVERLRAAGVDLPVIVVEAPRSVLGTIAARVYGRPSERLTVVGVTGTNGKTTTAWLIRSLMERAGYRCGLLGTVEYSDGISSGPATLTTPDSRSLAQWLGRMVQAGTRHAAIELSSHALHQGRAAGIELAAAIVTNVTQDHFDYHQTFENYLAAKARIIQHVRPGGIIVLNRDDPGAWSLREQVLESTTGISFGLNPTADVTAQIRDESLSGTRFRLKIHGESIDCATRLIGRHNISNILAAVAMLEAVGVPTQTAAEGVRAVSVPGRLEHVHDPAGRITALVDYAHTPDALRNVLETLRALLPDGHRLSCVVGAGGDRDRSKRPEMARIAAELADQVILTTDNPRSESPEAILDEMEAGVPPAAAPRVLRITDRRQAIRTAVRLGHEVLLVAGKGHEAGQTSAGVTRPFDDRAELAAALAERAGG",
    "response#3": "MKLIDILRGVPHTALRGEDVEVHGVAYDSRRVSPGSVFVALKGQHVDGAAFAREALARGAAAIVSEAPAPPGAEVPWTVVHDSRLALAILAVTFYHSHPATELWVVAVTGTNGKTTCTQWIAQSLNHLHRKCAVIGTLGQGLIDENGTNDAAINTTPDPVSLHGFLGQMRARGAKAVAMEVSSHGIEQGRVNGVDFDVAMFTNLSRDHLDYHGDMQSYGEAKAQLFAWPGLKHAVVNLDDQFGAELASRIDRKRINVIGYGFGKGEIAGHKLDLSTRGLKLEVATPWGAAKVRSGVLGTFNAHNILGVLGALLGLGYDLDALLAAAARLSPVPGRLEHVHDPAGRITALVDYAHTPDALRNVLETLRALLPDGHRLSCVVGAGGDRDRSKRPEMARIAAELADQVILTTDNPRSESPEAILDEMEAGVPPAAAPRVLRITDRRQAIRTAVRLGHEVLLVAGKGHEDYQEIAGHKRHFSDVEHARQALAFRQGRG"
  },
  {
    "reference": "MVREIFATITGRYDFLNRVLSLRRDVAWRRFAVRKMRFFDTFRLLDIATGTADLAIEAARRHPAIRVTGVDFVREMLSVGRLKLDRCGLEERIRLLQADALALPFPDESFDASSVAFGIRNMPDRLQALREMRRVLVPGGRVFVLEMNAPQNRLWKKVFTPYLKRVLPGVARLFSRNPAAYLYLVDSILHFPAPKVFLGMMEEAGLREVERHSLTLGITCLYIGQRKGQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein with S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity, S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity functions is required to enable aerobic respiration. The designed protein sequence is ",
    "response#1": "WSHFDHSIRTGEPAFDQVFGTDFFSYLSERPELSGTFTSSMREATRTMSTALAKEEEYDFSSYGTVVDIGGADGSLLAAVLSAHPGVEGVVFDSPEGARDAAATLDAAGVGERGRVETGDFFTRVPGGGDLYVLKSILHDWSDARSADILRTVRAAMPAHARLLVVEVLLPDTVDSSAHPLGYLSDLYMLVNMGGRERSERDLRSLLSDTGFR",
    "response#2": "WSHFDHSIRTGEPAFDQVFGTDFFSYLSERPELSGTFTSSMREATRTMSTALAKEEEYDFSSYGTVVDIGGADGSLLAAVLSAHPGVEGVVFDSPEGARDAAATLDAAGVGERGRVETGDFFTRVPGGGDLYVLKSILHDWSDARSADILRTVRAAMPAHARLLVVEVLLPDTVDSSAHPLGYLSDLYMLVNMGGRERSERDLRSLLSDTGFH",
    "response#3": "WSHFDHSIRTGEPAFDQVFGTDFFSYLSERPELSGTFTSSMREATRTMSTALAKEEEYDFSSYGTVVDIGGADGSLLAAVLSAHPGVEGVVFDSPEGARDAAATLDAAGVGERGRVETGDFFTRVPGGGDLYVLKSILHDWSDARSADILRTVRAAMPAHARLLVVEVLLPDTVDSSAHPLGYLSDLYMLVNMGGRERSERDLRSLLSDTGFR"
  },
  {
    "reference": "MPKIKTHRGAAKRFKKTATGKIRGWHAFHSHILGKKTAKRKRNLRKSTIIHESDAVRISRLLPY",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be involved in translation by localizing to ribosome, ribonucleoprotein complex and exhibiting structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "LKLQKRLAASVLRCGKKKVWLDPNEINEIANTNSRQNIRKLIKDGLIIKKPVVVHSRYRVRKNTEARRKGRHCGFGKRKGTANARMPTKLLWMQRQRVLRRLLKKYRDSKKIDRHLYHDLYMKCKGNVFKNKRVLMEYIHKKKAEKQRSKMLADQAEARRQR",
    "response#2": "IPPTTALVMKEAGVEKGSGTPNTQAVGNLPLEAVIRIAKMKMESMLSYDLKTAAKEVMGTCVSVGVTL",
    "response#3": "IPPTTALVMKEAGVEKGSGTPNTQAVGNLPLEAVIRIAKMKMESMLSYDLKTAAKEVMGTCVSVGVTV"
  },
  {
    "reference": "MTKYIFVTGGVTSSLGKGIIAASLAKLLQARGFRTTIQKFDPYINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERYLNTHTSQANNVTTGRIYQYVINKEREGAYLGKTVQVIPHITDEIKRRIMLLGNDDQFDIVITELGGTVGDIESLPYIEAVRQLKWELGDDNCLVVHLTLIPYLKAAKELKTKPTQHSVKLLSENGINPDILVCRTEEPLYKDLKRKIAQFCNVTQDSVIEALDADTIYSVPLEMMRERLDVICLQKLGLELGPDPELGLGKWKEFLNKLRYPKSKVTIGLIGKYVELQDAYKSILEALIHAGAMNECKVEVRNIHSEYLTPENAVEKLHNLDAILVAPGFGSRGIEGKIDAIRFARENKIPFFGICLGMQMACVEFARNVLNMATAHSTEMNPDTHNAVIAMMEEQKAITQMGGTMRLGAYDCELRDDSKAAKIYGSNRISERHRHRYEFNNEYIDAFEKAGMIPVGKNPKTGLVEIVEIPEHPFYVGTQFHPEYKSTVENPHPLFVAFVKAAKEAQEQRNNGNTHKVFEKTVA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+) binding.2. The protein's Nucleophile must be carefully engineered to ensure that the amino acids involved in H2O + L-glutamine = L-glutamate + NH4(+) are properly positioned for optimal catalysis.3. The protein must exhibit ATP binding, metal ion binding.4. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.. The designed protein sequence is ",
    "response#1": "LADSYVSVNQALVHAGAKIGKTVSIEWIDSETLNGNIENLAKFDGILVPGGFGVRGSEGIIKTANFAREKNIPYLGICFGFQLAAVAFGRRVCSFDDANSTEILPTTKHPIIDLLPEQKNVSNMGGSLRLGANQINVKENSLAHKIYKSNLITKRHRHRYEINKEYLGELTKKGMIFSAESDGGKRMEILEIPSHKFYLGVQFHPEFSSRPGFPEASFEAFL",
    "response#2": "TIAFVGKYVDLKESYKSLTESIIHAGATLDSRINLKWCDSEKIDASNVDELLRDVDGVLVAGGFGERGVSGKIEAIRYARENNIPYLGICLGMQLALIEFARNVLGLEDANSVEFNKDCANPIIYLIDSFIDASGAKQLRTHQSPLGGTMRLGGYECDVKPNSLLAKTYENAKTIKERHRHRYEANPDYRASFEAAGMSVSGESNGLIEAVEIAKHPFFLGVQFHPEFTSRLTKPNPAILGFVTIAMVGKYMELLDAYKSLIESLLHAGIKTRTKVNINYIDSEDIERDGTSVLESADAILVPGGFGERGVEGKIRTVQYARENKVPYLGICLGMQVAVIEYARNVAGLKDAHSTEFREHTPEPVVGLITEWLDATGEKEERTEESDLGGTMRLGAQDCVLTEGSTIVGCYGKKTIRERHRHRYEVNNHFLPRLEEAGLQISGRSADGKLVEVVEAPDHPWFVACQFHPEFTSTPRDGHPLFKGFVAK",
    "response#3": "ITIGLIGKYVELQDSYKSILEAFIHAGAENEVKVNIESVHSEYINNENAELKLGHLDGVLVAPGFGERGIEGKIDAVRYVRENNVPFLGICLGMQMAVIEFARNVLGIKDADSVEINSETKNPVIALMEDQKTVKDKGGTMRLGAWACDLKIGSKVRDIYKAESIAERHRHRYEFNGDYKEQFENAGMKATGLNPDTGLVEIVELQEHPWFVGVQYHPEYKSTVANPHPLFVAFVEAVSAG"
  },
  {
    "reference": "MPASLETCLSDLDCYLSGFLTDEEDCARLQPPGSASGPPAPPRRGPPGIPGAPDAPGAQDEQVRSEALLHSLRRSRRVKANDRERNRMHNLNAALDALRSVLPSFPDDTKLTKIETLRFAYNYIWALAETLRLADQGLPGGGARERLLPPQCVPCLPGPPSPASDAESWGSGAAASPCAAATSPLSDPSSPAASEDFAYGPSDPLFPFPGLSKDLLHTTPCFMPYH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design must be focused on enhancing the efficiency of genitalia morphogenesis, negative regulation of relaxation of muscle. The designed protein sequence is ",
    "response#1": "NRLQHCVSGETLVLSCPNTAHPHANTTWTFKRGGDYEPLQRDIGGYKFRRWSLVMTNVISKDSAMYKCTVCNTLGCIEFEFSVVVQ",
    "response#2": "SLISVDKLDLGSSNIFLIATFLNNKCEYNQVLLKSSTTIVQEVYASVLESILKLPIPEMRLLEYSNDEYIQMSRSLLTISSKTNKSLYDYIKLELLKSFFLIMEYAPNGKSFKDLDYKEYFSGYGGEKKLNQLGQVIAFDIFCNNSDRLPLIWDNVSSHFSNILFYDIPNRNGWYISLMNSNVTCINNSSFTIGYRKYITRVKSILYALFKRPNIESNQIKKMRESISKSYKINLSSSCGISIQRGIIQGC",
    "response#3": "SLISVDKLDLGSSNIFLIATFLNNKCEYNQVLLKSSTTIVQEVYASVLESILKLPIPEMRLLEYSNDEYIQMSRSLLTISSKTNKSLYDYIKLELLKSFFLIMEYAPNGKSFKDLDYKEYFSGYGGEKKLNQLGQVIAFDIFCNNSDRLPLIWDNVSSHFSNILFYDIPNRNGWYISLMNSNVTCINNSSFTIGYRKYITRVKSILYALFKRPNIESNQIKKMRESISKSYKINLSSSCGISIQRGIIQGE"
  },
  {
    "reference": "MSFQIRVFTAILAVLSLFTAPVLAQNSGPLRIEITEGVIEPLPFALPVFEAETADAADVAAQITQVIAADLTGTGLFRQIEPDAFISTVSSFAAPIQYADWKAINAQALITGAVAVQGNQLNVKFRLYDVFSGAEMGDGLQFSATPDGWRRMAHKVADAVYSRITGEGGYFDSRVVYVSETGTKDARQKRLAIMDYDGANVKYLTDSSSIVLAPRFSPDGQRILYTSYETGFPRIYVLDVTSLQRRGLESQEGTMSFAPRFAPDGQTIVFSLSQGGNTDIYRMNVNGGSATRLTSAPSIETAPSYSPDGTQIVFESDRSGSQQLYVMPANGGEARRISFGPGRYGTPVWSPRGDLVAFTKQNAGRFHIGVMRLDGSEERLLTASFLDEGPTWSPNGRVIMFSRETQGAQGRATLYSVDITGRNLRPVRTPEGGSDPSWSPLQR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have a specific function in protein import and should be localized to periplasmic space in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "GNRLGILVTTYVNAINSGAVPCLDDAVTTLAQRENSVAVQKAADHYSEQMAQRLRLPTETLQELLDVHAACEKEAMAVFMEHSFKDENQQFLKKLVELIGENKELFLSKNEEASNKYCQEELDRLSKDFMENISTFFVPCGHKLYMDKREKIEHDYWQVPRKGVKASEVFQSFLQSQAFIESSILQADTALTAGEKAIAEERAQKVAAEKEQELLRQKQKEQQEYMEAQEKSHKENLEQLRRKLEQEREQDIKDHDMMLKKLMKDQKAFLEEGFKKKAEEMNKEIQQLL",
    "response#2": "EGIKVTGNRLGILVTTYVNAINSGAVPCLDDAVTTLAQRENSVAVQKAADHYSEQMAQRLRLPTETLQELLDVHAACEKEAMAVFMEHSFKDENQQFLKKLVELIGENKELFLSKNEEASNKYCQEELDRLSKDFMENISTFFVPCGHKLYMDKREKIEHDYWQVPRKGVKASEVFQSFLQSQAFIESSILQADTALTAGEKAIAEERAQKVAAEKEQELLRQKQKEQQEYMEAQEKSHKENLEQLRRKLEQEREQDIKDHDMMLKKLMKDQKAFLEEGFKKKAEEMNKEIQQLL",
    "response#3": "EGIKVTGNRLGILVTTYVNAINSGAVPCLDDAVTTLAQRENSVAVQKAADHYSEQMAQRLRLPTETLQELLDVHAACEKEAMAVFMEHSFKDENQQFLKKLVELIGENKELFLSKNEEASNKYCQEELDRLSKDFMENISTFFVPCGHKLYMDKREKIEHDYWQVPRKGVKASEVFQSFLQSQAFIESSILQADTALTAGEKAIAEERAQKVAAEKEQELLRQKQKEQQEYMEAQEKSHKENLEQLRRKLEQEREQDIKDHDMMLKKLMKDQKAFLEEGFKKKAEEMNKEIQQLL"
  },
  {
    "reference": "MDFLPIFMNVKDRLCLIIGGGEVAQRKASVLLDAGAMVKAVAPEFLEEFSGLAGIEYVVQRFQPNHLDGAILVIAATDDHVVNHEVSKLARARNIPVNVVDNPDLCTFIMPSILDRSPLMVAFSSGGASPVLARMLRGKLETMIPQGYSRLTAFCARLRNTVRTGITEPAIRRIFWENVLEGTIAEKVLAGDEADAEAMLLDQLKKGNKPPSGEVYLVGAGPGDPDLLTFRALRLMQKADIVLFDNLVSKPIVEMTRRDATRIYVGKQRNNHTLPQEEINVLLVRLAQEGKRVLRLKGGDPFIFGRGGEEIETLAAHGIPFQIVPGITAASGVASYAGIPLTHRDYAQSCTFVTGHLKNGTTNLDWERLARPNQTIVFYMGLHGLETICAQLVSHGLPAGTPAALVQQGTTQDQRIYTGTLATLPEIVQTEKPRPPTLIIVGGVVTLREKLNWFHPEKSE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of Tetrapyrrole methylase domains is critical for the interaction of the designed protein with its intended targets.2. The designed protein should have a high specificity for S-adenosyl-L-methionine, NAD(+) binding, with minimal off-target effects.3. The designed protein must possess sirohydrochlorin ferrochelatase activity, precorrin-2 dehydrogenase activity, uroporphyrin-III C-methyltransferase activity, NAD binding to support siroheme biosynthetic process under certain conditions.4. The design of the protein must focus on the precise arrangement of amino acids in the Proton donor, Proton acceptor to enable effective catalysis of NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin.5. The protein design should consider the endogenous proteins and enzymes present in the siroheme biosynthesis, sirohydrochlorin from precorrin-2: step 1/1, Porphyrin-containing compound metabolism pathway to avoid interference with native metabolic processes.6. For general function, the protein need meet that Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin; Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1 The designed protein sequence is ",
    "response#1": "LPIFVNIKGRLCLVVGGGEVAQRKASVLLDAGAAIKAVAPEFSAAFAGLPGVECIVERFQPGHLDGAVLVIAATDDSAVNQDVSREARARNIPVNVVDNPDLCTFVMPSILDRSPLLVAFSSGGASPVLARMLRGKLETMIPQGYGRLTAFCARLRGTVKERVTNPPMRRVFWENVLEGAIAEKVLAGDEAGAEAMLLGQVGSGPGDPDLLTFRALRLMQRADVVLYDSLLSPDILALVSPEAERIHVGKRAERHTLPQERINTLMVELAQQGRRVLRLKGGDPFVFGRGGEEITQLAEAGIDFQIVPGITAANGCAAYAGIPLTHRDHAQSVIFVTGHLQAGELKLGWAELARPGQTVVFFMGRRNLALICDRLREYGLADDWPAAMIIDGTTARQTIIAGTLADLPERVAADPAKGPALLIVGEVLAA",
    "response#2": "LPIFVNIKGRLCLVVGGGEVAQRKASVLLDAGAAIKAVAPEFSAAFAGLPGVECIVERFQPGHLDGAVLVIAATDDSAVNQDVSREARARNIPVNVVDNPDLCTFVMPSILDRSPLLVAFSSGGASPVLARMLRGKLETMIPQGYGRLTAFCARLRGTVKERVTNPPMRRVFWENVLEGAIAEKVLAGDEAGAEAMLLGQVGAGPGDVGLLTLRGLQVMQQADVVLYDHLVSDETLDLVRRDAERICVGKRAGAHSVIQEETNRLLVELAQQGKRVVRLKGGDPFIFGRGGEELQVAAAAGIPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCRPDGSGLDWADLARARQTLAIYMGTMKAADISRNLIAHGREGNTPVAVISRGTRPDQQVQTGTLQQLEQLAQQAPLPALLVIGEVLAEAGSPGLGEVYLVGSGPGDPDLLTFRALRLMQRADVVLYDSLLSPDILALVSPEAERIHVGKRAERHTLPQERINTLMVELAQQGRRVLRLKGGDPFVFGRGGEEITQLAEAGIDFQIVPGITAANGCAAYAGIPLTHRDHAQSVIFVTGHLQAGELKLGWAELARPGQTVVFFMGRRNLALICDRLREYGLADDWPAAMIIDGTTARQTIIAGTLADLPERVAADPAKGPALLIVGEVVRLVGAGPGDPGLLTLRALRLLNQADVILHDRLVGPGVLALARRDAEFIEVGKQAANHHVTQDGIHALMLEHARAGRRVVRLKGGDPFVFGRGGEELEFLHAHGIAFEVVPGVTAALACAAYAGVPLTHRDHAQSVRFVTAHCKDSLDTLDWRALAQEKQTLAVYMGVAGLASFRDRLLAHGRAASTPFALVENGSRPEQRVVIGTLDRLPETAAAHAVQSPALLILGEV",
    "response#3": "LPIFANLRDRQCLVVGGGEVAWRKTRMLLKTGAAVTLVAPEVDKEIEEAAEKGVLTWIAEPFSAKYLDGVFLAIAATDQPLVNALVSRSAEEKKLLVNVVDDTPKCSFIVPSIVDRSPIVIAISSAGKAPVLARLIREKLESLIPSHTGRMAAIAGSFRDTLKEKVTTLTGRRKFWEMAFDGRFSSLVAAGREDDAREELHDALTTSFFGEDLGEVALVGAGPGDPGLLTLRGLQLMQQADVILYDYLVSDDVLELCRRDAEMICVGKRAGFHSVPQEETNALLVKFASRGKRVVRLKGGDPFMFGRGGEELQVVAEAGIPFQVVPGITSAAGATAYAGIPLTHRDHAQSVMFITGHLKKEKDSLDWSTLARGNQTLVIFMGLMKSDHIRDQLIKHGRAAHTPVAIIENGTRSEQRVLKGTVHDLATLAEHASSPALIVVGEVVQLGEIILVGAGPGDSGLLTLRALQVMQLADVVLYDYLVSKEVLEMVRRDAEFICVGKRAGYHSISQEKTNDLLIKLAKQGKRVVRLKGGDPFIFGRGAEELQAAYQAGIPFQVVPGITAAIGASIYAGIPLTHRDYAQKVIFITAQGKTNKNNIDWLSLACERQTLAIYMSAMKAAEISAELIKHGRSPQTPVAVISSGTNKNQQVLIGQLKYLEKLSTNTLSPTLLVIGEVVDLLHDDLAWVGAGPGDPDLLTFRALRLMQQADVVVYDRLVAKSILALVRRDARHIYVGKERNAHTMRQEEINALLIQLARDGLRVLRLKGGDPFIFGRGGEEIDTLAAEGIPFQVVPGITAASGCACYSGIPLTHRDYAQSVTFVTGHLKDQTIDLNWPQLAQPNQTVVFYMGLVGLRTIIQQLLAHGVSPQMPIALVQQGTTHMQRVYIGTLADIEAQIQHDPPQPPTLIIVGEV"
  },
  {
    "reference": "MVLLVRKLYRSFLILFHLFLWCKLPGARWFPKTLPCNVTQNIMEASVMVNCSDKHLTEIPKGIPYNTTNLTLTINHIPKITPNSFDDLKNLVEIDFRCNCIPVRLGPKDHICLKRPKISFGSFKNLTNLKSLYLDGNQLTEIPLGLPSNLQLLSLEANHIFSITKENLTELDNLEILYLGQNCYYRNPCNVSFFIEKDAFQSLRNLTVLSLKADNLTTVPTTLPSSLRELYLYNNIIQSIEKDDFQNLNQLEILDLSGNCPRCFNVPFPCIPCPNNSALKIHNNAFDSLIELKFLRLHSNSLQNIPKSWFKNTKKLEELDLSQNFLAKEIGDAKFLYSLASLIILDLSFNYEPQIYRAYLNLSKSFASLKNLKILRLKGYVFKELKSNNLLSLQKLSNLEVLDLGTNFIKIAELGVLKKFQALKNIDLSVNKISPSGEPSQNGFCSMSRTSTEFHVSQVFESMHYFRYDEFARSCMFKSKEKPSLSLINNDCESYGKSLDLSRNNIFFINPSDFQDLSFLECLNLSGNSISQTLNGSEFHPLVKLKYLDFSNNKLDLLYPTAFQELQELEVLDISGNSHYFLSEGITHMLNFTKNLPVLKKLMMNGNDISTSTSTKMESESLSILEFRGNRLDILWRDGDNRYLQFFRNLNKLEELDISENSLHFLPLGAFEGMPPNLKTLFLSKNNFKSFTWDNLIYLKNLETLDLSHNLLTSVPEVLSNCSKSLKKLILNNNYIRHLTKNFLQDAFQLRYLDLSSNKLHVIQKSSF",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have siRNA binding, single-stranded RNA binding within receptor complex, plasma membrane, endosome, lysosome to regulate positive regulation of interleukin-6 production.2. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "DLEKRIFRGYDIKKRPVKNASVPTVVDVHWHVIHVSINQKEQTMTLHGHIYMRWYDEYLVWDPKDFAGIHYARVKKWQVWQPKIRVSNSASGLASAFDFSTSAHVIIQMVEKDRAKVEMYPTFSIKVGCMFDFGDFPYDQNKCSVNLFATDTMAEVQLQ",
    "response#2": "DLEKRIFRGYDIKKRPVKNASVPTVVDVHWHVIHVSINQKEQTMTLHGHIYMRWYDEYLVWDPKDFAGIHYARVKKWQVWQPKIRVSNSASGLASAFDFSTSAHVIIQMVEKDRAKVEMYPTFSIKVGCMFDFGDFPYDQNKCSVNLFATDTMAEVQLI",
    "response#3": "DLEKRIFRGYDIKKRPVKNASVPTVVDVHWHVIHVSINQKEQTMTLHGHIYMRWYDEYLVWDPKDFAGIHYARVKKWQVWQPKIRVSNSASGLASAFDFSTSAHVIIQMVEKDRAKVEMYPTFSIKVGCMFDFGDFPYDQNKCSVNLFATDTMAEVQLN"
  },
  {
    "reference": "MNSYRSVYYLLRMSQGEPIIKSRFFTSQQSKKSPITIEYEDEPSEKFTKLSSTTTTTTTTNTLDESKKINSSGTKRKIEDTKKKFESMSLARTEWEDVWKKIEKQRKETEAPVDVMGAESFDDHKFEDKERRFHVLVGCLLSSQTKDQITHAAMKKLIDYGLNVDNILKTSESKIEELLHPVLYYKRKAIYLKKISQILKDKYDSDVPSTYEELMALPGIGPKMTLLILNIAWKKLVGIAVDTHMHRISNRLGWVNTTQPEETRKSLESWLPKDRWDQVNLLLVGFGQTICLPQKPKCLACSVNNQCPTGIKNLNSNDNEKEEEEEEEEKEKKKKSSKKIKSSNK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must have a Nucleophile; for N-glycosylase activity active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "TESYKSLNEALVHGGIANDTHVNLKFVNSTDLQEEQGDISKYFEDIHGVLVPGGFGVRGTEGKILAIKYARENGIPLFGICLGMQLAVVEFARHVCNMENANSTEFDADTPWPVIDLLPGQISIERKGGTMRLGAYKCVLTPNTRAMEAYKSNVISERHRHRYEFNNKYEETLVEHGLVVSGKNPETSLVEIVELKDHPWFLGCQFHPEFKSKPMNPHPLFREFIR",
    "response#2": "TESYKSLNEALVHGGIANDTHVNLKFVNSTDLQEEQGDISKYFEDIHGVLVPGGFGVRGTEGKILAIKYARENGIPLFGICLGMQLAVVEFARHVCNMENANSTEFDADTPWPVIDLLPGQISIERKGGTMRLGAYKCVLTPNTRAMEAYKSNVISERHRHRYEFNNKYEETLVEHGLVVSGKNPETSLVEIVELKDHPWFLGCQFHPEFKSKPMNPHPLFREFIR",
    "response#3": "TESYKSLNEALVHGGIANDTHVNLKFVNSTDLQEEQGDISKYFEDIHGVLVPGGFGVRGTEGKILAIKYARENGIPLFGICLGMQLAVVEFARHVCNMENANSTEFDADTPWPVIDLLPGQISIERKGGTMRLGAYKCVLTPNTRAMEAYKSNVISERHRHRYEFNNKYEETLVEHGLVVSGKNPETSLVEIVELKDHPWFLGCQFHPEFKSKPMNPHPLFREFITESYKSLNEALVHGGIANDTHVNLKFVNSTDLQEEQGDISKYFEDIHGVLVPGGFGVRGTEGKILAIKYARENGIPLFGICLGMQLAVVEFARHVCNMENANSTEFDADTPWPVIDLLPGQISIERKGGTMRLGAYKCVLTPNTRAMEAYKSNVISERHRHRYEFNNKYEETLVEHGLVVSGKNPETSLVEIVELKDHPWFLGCQFHPEFKSKPMNPHPLFREFIQR"
  },
  {
    "reference": "MLYKRSSQLFLEAEKVIPGGVNSPVRAFKSVGGTPIFAKSAKGAYVYDEDGNRFVDYINSWGPMILGHAYEPVVTAVIEKAKSGTSFGMPTELETEIAKLAVSMVSNIDKIRFVNSGTEACMSAIRLARGFTKRDKIIKFAGCYHGHSDSFLIQAGSGAITFGSPNSPGVTSGTAKDTLLASYNDIQNVKNLFDANKNEIAAVIIEPVAGNMGCIPPQKGFLEALQQLCHENNALLIFDEVMTGFRLAKGGAQELFNVQADIVCFGKVIGGGLPVGAFAARNEIMNYLAPLGPVYQAGTLSGNPLAMAAGLAMLKALNENQEVFARLEEKTAYLAKGIADVLTSNNVVHTINRVGSMVSVHFDANPVFDFETAKNGDNDTFKKFFHGLLAEGVYIAPSAYETWFISDALTYEDLDFTIRAVDKVSKNL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have a low Km value for (S)-4-amino-5-oxopentanoate = 5-aminolevulinate.2. The designed protein should be involved in protoporphyrinogen IX biosynthetic process by localizing to cytoplasm and exhibiting transaminase activity, pyridoxal phosphate binding, glutamate-1-semialdehyde 2,1-aminomutase activity.3. The protein design should incorporate a binding pocket that specifically recognizes pyridoxal 5'-phosphate to enhance enzymatic efficiency.4. A protein that can modulate the overall flux of metabolites through the 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2, Porphyrin-containing compound metabolism pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MSEINHFGFKSVAESEKAGKVAEVFHSVAAKLIFDEVMTGFRLAAGGVQERAGVKADIVCFGKVIGGWRRFAVDMAGVRPGQRVLDIAGGTGDLSFKFGPLVGPTGQVILSDINSSMLRVGRDRLLDQGVAGNVQYVQADAETLPFPDNYFDCVSVAFGLRNVTDKQKALASMYRIMKPGGRLLILECSKPVLPGLSRIYDFYSFQVLPRLGKLVAGDEASYRYLAESIRMHPGQEELKGMMREAGFARVQYFNLTGGIVALHRGFKF",
    "response#2": "MIQTILIATKNKGKLREFQEMLSPLSFEILSLDDFPDLPQVVEDGKTFEENALKKASHTANIFGLPTVADDSGLCVNALNGMPGVFSARYAGENATDADNNAKLLAELKDVPEAHRTAQFICVCALVTPTGEIHTAAGDCNGTIIDEQRGSNGFGYDPLFIPDGFAQTFAELGTEEKNAISHRGKALQALAEQIQALT",
    "response#3": "MEIILANPRGFCAGVDRAISIVERALELYGAPIYVRHEVVHNRFVVDGLRDRGAIFVEELSEVPDDAVVIFSAHGVSQAVRAEARSRQLTVFDATCPLVTKVHMEVARASRKGKEAILIGHAGHPEVEGTMGQYSNPEGGMYLVESPEDVWSLQVKNEEKLSFMTQTTLSVDDTSEVIDALRKRFPNIVGPRKDDICYATTNRQEAVRALAQDCELVIVVGSPNSSNSNRLREVAERVGAKSYLVDNADKIEQDWFAGIAQVGVTAGASAPEILVQQVIEKLRAFGYEDVEVLTGREENIVFALPAELRVELA"
  },
  {
    "reference": "MFEPLPVPAEMAEWDQSAIHEIGIRGELLMENAGREALNVLRQACGPLSNKRVLVLAGPGNNGGDAFVVARHLHDLGANVLLLHSRAQSAYKGHAGYHLRLAKRIGTPIRRLTPSLFRRYHQKGSISLPNGISFSGSPEILIDGLLGTGFEGSLRADMLEIVQLANQMTTNAVVLALDIPSGLNGATGMPQPIAIRADLTATFGAVKLGLALPEAAEYTGLVAPCDIGIPNQCLQSAPPRHFRIAPEIFDIAPKANPNAHKGQNGSLLVVGGSRGLTGAPHLAALGALRAGCGLVTVAAPAALCRDIKLNHPEIMTLPLGQDCRWSSALAEELVTHLNNNPARYHALAVGPGLGRSDGARHFLSVIGRSLTLPTVYDADALFHLSNLHQTLPAQAVITPHPGEAARLLGCSTREIQADRITAARQLATLFHTVAVLKGAWSLVADPPGRIHLCDMAVPSLAVAGSGDVLTGLTGYLLAMGFSPLHAACLGVYWHASAGKHLDQLRHRGHLAGEIANALADARHQYNKEASC",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. The designed protein should have a binding site that is specific to the target K(+), AMP, (6S)-NADPHX ligand.3. We need a protein that can perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate with high efficiency.4. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.5. The protein must be able to NADPHX epimerase activity, ATP binding. The designed protein sequence is ",
    "response#1": "MRAIEQAAIESGEVTGLELMERAGQGVVDAVFEEWPELRKRGASPSRSPGYLGPDEGDPLRAVVLCGPGNNGGDGFVVARLLRGLGWEVEVFLYGDAEKLPPDAKKNFARWSASNVVHDWDDEEIEGILDEQEHDLVVDALFGTGITRSMPDDTDRTWNGFVPTVTGTLPQDRRPKYVAVDCPSGLCCDSGMNFGALPTDLTVSFHSPKLGHFLMGDGHYGGGPGLSGKLVISDIGLKPAYAEAQDVRDWMSSTAFSSDSYKKSRGSVLIAAGSKKYSGAAVLSANGAMRSGAGIVTAAVPRSAFSAVAERVLPEVMVREVSDTEVGTFGVNSYGGIEDLVRGSHTVLVGCGIAMENGVTEFVQTLIARRLQPLVIDADGLNAISPISVEGIEEAPLILTPHIGEFKRLAGIENEISDDKRVEVLRGFAVDNQVICVLKGERTLIASPEGVVVVNPTGNPGLGKAGNGDNLAGIISGFVAQTVVARDSMSGNDVEEEVFGAVVAAVYVAGLAGDIAAEKFGNRVMTASDVRECLTDAFE",
    "response#2": "MIEAWTADEIRAAEKPLLDAGVPLMDRAARAIARAALADVARRRSGGGAPRPRFAGFLGSDGVPGPGRVRPPRRPGAVGNAAHGARAVLLVGAGNNGGDTLFAGAHLARSGMHVTAFLVAGVVHGEGLAALEAAGADVIARSELRVPVVVEHVEAADVVLDGLVGIGSRGALRPPSGGIVRALVESRSAAETAGTPWPWVVAVDVPSGIGVDDGSVPGPVLPADRTVTFGARKPGLILPPAAHLAGVVQVADVGLGTAQGEPADYADVVAPHTNNVDKYSRGSVLVVAGSSRYPGAAVMAAKAAARAGAGYVTLAVPVTIAPLIQMQLPEVPVVGVAAEQDGTFSAAARPVILKLAERSSATLVGPGMRVTGGTVAVVSALINSKAPLVVDADGLNCLARLTNGHLDEFPEVTRRDAPLVLTPHRRELGRLVGLADTPPDSLTAALEAARRIVWADGGSELCIVAKGTATACVGVEAALLPEPGTASLATAGSGDVLGGIICALLARMPDDIEDLPLIAAFGCEVHAVAGSRAAKRYGSRGVMAGDIADEVGLAVDEL",
    "response#3": "MRQIENQAFGAGISSDHMIQTVGLKIANILDSDCSDKNKMVLGLIGKGNNGSDTLAILTYLAQKGWETFAWLLSKRESEDRYLKDFLNVSGKAVDYENDTEESQLDNWLKQSSIILDGIYGIGFHLPLGEAFRPIFRKINQRKNPSLIYAIDCPSGMDCDTGKIDPDCIRANRTICIEAVKIGEVLPPANLLVGELETISLDLPQDLTDHKYTRGVVGVVAGTEKYPGAGVLVTAGAVRAGAGMVRALGPTTVTDRILTRHPEVVTAGGRVQSWVLGPGIPSDGSDAGQDRRVYGALAAVTGVLDAEVTGGVVPAVIDAGAMAMLEHRLPEIAVLTPHARELAELMRGLGHEVDRADVEAEPVRWARAAQRATGATVLLKGSVTVIVGSGATFTQADAPAWLATAGAGDVLAGLLGTLLASRADDVLADPTLTSRVAAAAVVLHGRAAALASGGGPVSATDVADAVPSLWLHHAATPLTAHHKYDRGHAVVFSGGMSQTGAARLAALSALRGGAGLVTVFSPASALLVNAQHLTGIMLKRCEDGDLDVLLEDKRLNAFVLGPGFGIGDRAKAMALTVLKAGRRLVLDADGLTSFRDHASQLFEAARQCSHSEPQLVLTPHDGEFARLFPDLAGDLRLSKVDRARHASRRSNAIVVLKGADTVIAAPDGRAAINATGTPWLATAGTGDVLAGLVAAQLAQKRPSFEAACRAVWMHGKAAEHFGPGLISEDLPHCLPAVHRELVAQLAGPAPVRVRAHASHKGLNGDVAVIGGAPGMAGAAVLAATSALCGGAGRVFLAPLDTGFGAAVPGWPELMVRAWDALPLESISVACGCGGGDAVAAMLPAVLSRAKWLVLDADALNAIAADASLQETLRRRGRPTVLTPHPLEAARLLGTSTAQVQGDRLRAAQELGERSKAVVVLKGSGTVIAAPGTTPSINPTGNGRLATGGTGDVLAGLVAAALPVSSDALQAAAHAVYRHGKAADDWPAGEPLTAGALARRLHL"
  },
  {
    "reference": "SRMSDPKMIKEIIDAVSIPVMAKVRIGHVVEAQILQAIGIDYIDESEVLTPADDLFHINKKEFNVPFVCGARNLGEALRRIGEGACMIRTKGEAGTGNVIEAVRHMRTIQSQIRKLKVMPKEELMIAAKELGAPYDLVEYVRENGKLPVINFAAGGIATPADAALMMQLGCDGVFVGSGIFKSENPAKRAKAIVEAVKNYNNPLKIAEVSEGLGEAMTGLEIDKLDVTFAER",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The Schiff-base intermediate with D-ribose 5-phosphate active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The protein design must be focused on enhancing the efficiency of pyridoxal phosphate biosynthetic process.3. For general function, the protein need meet that Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia; Can also use ribulose 5- phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.; The ammonia is provided by the PdxT subunit4. We require a protein that can specifically bind to the metabolites produced in the Cofactor biosynthesis, pyridoxal 5'-phosphate biosynthesis pathway. The designed protein sequence is ",
    "response#1": "LLKHGWCEMLKGGVIMDVKSVEQAKIAEEAGAIGVMVLENIPSELRNKEGVARSVDPSKVEEIKKCVSINVLAKVRIGHFVEAQILEELKIDMIDESEVLTIADEMHHIDKHKFKTPFVCGCTNLGEALRRISEGASMIRTKGEAGTGNIIEAIKHIRTVNNEIKYLCSLSDSEVYHFAKKINAPIDLVLLTKKLKRLPVVNFATAHVKRGMAEMLKGGVIMDVVTAEQAKIAEDAGAVAVMALERVPSDIRAQGGVSRMSDPDMIDSIMDAVSIPVMAKVRIGHFVEAQILQSLGVDYVDESEVLTPADYANHVDKWAFTVPFVCGATNLGEALRRITEGAAMIRSKGEAGTGDVSNATTHMRGIRTEIRRLQSLPTDELYLAAKDLQAPYELVKEIAETGKLPVVLFTKEPRDDELIDYLEFDELLKHGWCEMLKGGVIMDVKSVEQAKIAEEAGAIGVMVLENIPSELRNKEGVARSVDPSKVEEIKKCVSINVLAKVRIGHFVEAQILEELKIDMIDESEVLTIADEMHHIDKHKFKTPFVCGCTNLGEALRRISEGASMIRTKGEAGTGNIIEAIKHIRTVNNEIKYLCSLSDSEVYHFAKKINAPIDLVLLTKKLKRLPVVNFAEVTSSYS",
    "response#2": "VRYGFISMLKGGVVMDVTNEQQAEIAEDAGAVGVMVLDKLPYDVRKAGGVARTADLNVIRAVMETITIPVSAKCRIGHYWEAKLLEEIGVDLIDESEVLTPTDEKAHIWKWEFRVPFVNGARSLPEALRRIWEGASMIRTKGEAGTGNVAEAVRHMKAVNRDIAVLRGYYKAGDIEAIRLYAKSNNVPFELALLTARLGRLPVVNFATAHVKRGMAEMLKGGVIMDVVTAEQAKIAEDAGAVAVMALERVPSDIRAQGGVSRMSDPDMIDSIMDAVSIPVMAKVRIGHFVEAQILQSLGVDYVDESEVLTPADYANHVDKWAFTVPFVCGATNLGEALRRITEGAAMIRSKGEAGTGDVSNATTHMRGIRTEIRRLQSLPTDELYLAAKDLQAPYELVKEIAETGKLPVVLFTAGTRGDGIHGGQMAAMVGLEAWTRALPR",
    "response#3": "MSVRYGFISMLKGGVVMDVTNEQQAEIAEDAGAVGVMVLDKLPYDVRKAGGVARTADLNVIRAVMETITIPVSAKCRIGHYWEAKLLEEIGVDLIDESEVLTPTDEKAHIWKWEFRVPFVNGARSLPEALRRIWEGASMIRTKGEAGTGNVAEAVRHMKAVNRDIAVLRGYYKAGDIEAIRLYAKSNNVPFELALLTARLGRLPVVNFATAHVKRGMAEMLKGGVIMDVVTAEQAKIAEDAGAVAVMALERVPSDIRAQGGVSRMSDPDMIDSIMDAVSIPVMAKVRIGHFVEAQILQSLGVDYVDESEVLTPADYANHVDKWAFTVPFVCGATNLGEALRRITEGAAMIRSKGEAGTGDVSNATTHMRGIRTEIRRLQSLPTDELYLAAKDLQAPYELVKEIAETGKLPVVLFTA"
  },
  {
    "reference": "MKKYEIMYILRPNLDSKDVKKINDTLQNVFLQAPNQILEQNEIGLKDLAYLIDNHKKGYYNWLMVKADNDAVLEFNRIVKITEEIIRFIFIKDKE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that contributes to translation by functioning in ribosome with rRNA binding.2. For general function, the protein need meet that Binds together with S18 to 16S ribosomal RNA. The designed protein sequence is ",
    "response#1": "SRLDNLVYRLGLARTRRQARQLVTHGHIIVDGSRVNIPSYRVKPGQTIAVREKSRNLQVIKEALG",
    "response#2": "LESRLDNLVYRLGLARTRRQARQLGG",
    "response#3": "MPSRLDNLVYRLGLARTRRQARQLVTHGHIIVDGSRVNIPSYRVKPGQTIAVREKSRNLQVIKEALS"
  },
  {
    "reference": "MPERRPTDRPLTVAVVGATGLVGRTMIQLLAEREFPVGELRPLASRSAGREVELDGRRFAVAEATPEAFEGVDIALFSAGADISKVLAPEAAARGVLVIDNSSQWRMGPDIPLVVPEVNPEDAAEHEGIIANPNCSTMQLVPVLMALRDSVGLERVVVDTYQSVSGTGVDAVDELEGQIRAHANGERPEARVYPHPIAFNALPEIDVFMDDGYTKEEWKVIHESRKILHLPELRVSCTAVRVPVFYAHSEAVHVETRESITPARARELFGQVPGVVVQDDPGSHLYPLATSAQGSDEVYVGRVRRDRSIADGRGLAFWVVSDNLRKGAATNAIQIAELVRRNGWIQKRSERGMDVAKAS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have NADP binding, protein dimerization activity, NAD binding to facilitate diaminopimelate biosynthetic process, isoleucine biosynthetic process.2. We require a protein that can specifically bind to the metabolites produced in the Amino-acid biosynthesis, L-threonine biosynthesis pathway.3. The protein should be able to bind phosphate, substrate ligand in a variety of conditions4. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.5. The designed protein is the optimization of the Proton acceptor, Acyl-thioester intermediate to achieve high catalytic efficiency for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH. The designed protein sequence is ",
    "response#1": "MANNVTILGATGAVGQQMMQCLEERDFPVGELRLLASARSAGAIIAWNGRLVRVEEATPDSFAGADIVLGAADNPVAEALLPHAVAAGACVVDNSSAFRLGPDVPLVIPEINPQDVALDADRIITANCNTLGGLFEADLIEWMTSMTYQAASGSGAKHMRELLNQMGALRDSVNSELSDPVSAILDIDRRVAASMRGGDFPIDNFGAPLAGGLLPWIDKPMENGQTREEWKGSVETNKILGLGDNPIPIDGLCVRIGAMRSHSQAFTIKLKRDVPIDEIEERIATHNDWVKVIPNEKEATLAGLTPAAVTGTLRIPVGRLRKMAMGGEYLTAFSVGDQLLKGAALNAVQIAEVLLG",
    "response#2": "MNNIAIVGVTGAVGVKMLEVLNSRKFPIKELNVFASERSKGKTITFRNEEYTIKTIQDDSFENTHFVFCSADSNISKKIGPQVRKAKSILIDDGNAFRMDDDVPLIVPEVNKQDLDWLHREYGIKRIVVSSYQAVSGTGKKAIDELHDQTRAILNYLDYEAKVYPHQISFNCLPHIDVFLDNGYTKEEMKMVNETRKILEDDSIGVTATTVRVPVFYGHSESVNVETKKPISAAEYRELLKSQPGVKVVDDPSKNEYPLAIDAAGQDLTLVGRIRDDESIENGINMWIVADNIRYGTTVVPLGGLFEADLIEWMTSMTYQAASGSGAKHMRELLNQMGALRDSVNSELSDPVSAILDIDRRVAASMRGGDFPIDNFGAPLAGGLLPWIDKPMENGQTREEWKGSVETNKILGLGDNPIPIDGLCVRIGAMRSHSQAFTIKLKRDVPIDEIEERIATHNDWVKVIPNEKEATLAGLTPAAVTGTLRIPVGRLRKMAMGGEYLTAFSVGDQLLKVGFVGWRGMVGSVLMQRMSEDNEFTGIEPVFFTTSQVGQPGPDVGVDVPPLKDAFDIDALKALDVIITCQGGDYTGEVYQKLRQSGWKGYWIDAASTLRMTDDATIILDPVNRHVIDALHALNKHLPIQRVNVSTYQAVSGAGAQAVQELKSQIKQIINQEKIIPSVFSEQIAMNVIPQVDDFESNGYTKEEMKIVNETRKILHLDNLAISATCTRVPTMVGHCESVLVEFNQEVNLDKIIQIISNQEGVVHRDLDNIDNYPTPLNATLDDNVYVGRIRKDLSNINSVLFWLVSDNLL",
    "response#3": "MLNVAIAGATGAVGNQMIQCLEERNFPMKSLKLLASERSAGRKIRFKGDDLTVELLTKDSFKDVDVAIFSAGGTPSKIFAPAAAEAGCVVVDNSSAWRMDPDIPLVVPEANPHAIAGYTNRGIIANPNCSTIQMLVALAPIHALSPIERVHVATYQAASGAGHDAMLELEGQVRDQAMGKPLQTKAFGNQLLYNLIPQIDIFTDNDYTKEEMKMYNETCKILHNDEIRVSATCVRVPVLRAHSEAIWVKCAEPLTVEAVREAMSKQKGLLLMDDPAKRSYPMPLHCSMQEPVYVGRLRADLAEPGCVTFWCVADQIMKGAALNSVQIAEVLIRDYL"
  },
  {
    "reference": "MEPVDPNLEPWKHPGSQPKTACTNCYCKKCCLHCQVCFLTKGLGISYGRKKRRQRRRASKDSQTHQSATSKQPTSQPRGDPTGPEEQKKKVETETETHQGP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: In the cytoplasm, Tat is thought to act as a translational activator of HIV-1 mRNAs.; Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to promote processive transcription elongation by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts; Through its interaction with host TBP, Tat may also modulate transcription initiation; Besides its effect on RNA Pol II processivity, Tat induces chromatin remodeling of proviral genes by recruiting the histone acetyltransferases (HATs) CREBBP, EP300 and PCAF to the chromatin; In the absence of Tat, the RNA Pol II generates short or non-processive transcripts that terminate at approximately 60 bp from the initiation site; This also contributes to the increase in proviral transcription rate, especially when the provirus integrates in transcriptionally silent region of the host genome.2. The protein design should include a Cell attachment site motif to ensure proper function.3. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding. The designed protein sequence is ",
    "response#1": "MEPVDPSLEPWKHPGSQPRTACNNCYCKKCCFHCQVCFITKGLGISYGRKKRRQRRRAPQDSQTNQVSLSKQPASQPRGDPTGPKESKKKVESETETDQD",
    "response#2": "MDPVDPNLEPWNHPGSQPTTACNNCYCKMCSYHCQVCFLNKGLGISYGRKKRRQRRRTPQSSEDHQNPISKQPLSQARGDQTGSEESKKKVESKTEPDPRD",
    "response#3": "MDPVDPNLEPWNHPGSQPKTPCNKCYCKKCCYHCQVCFIHKGLGISYGRKKRKQRRRTPQDSQTHQVSLPKQPTSQPRGDPTGPKEXKKKVETETETDPLD"
  },
  {
    "reference": "MSNTEQHQKKGQKNKVLLNNDIPFSKVFLIGEDGEKIGIKTKEEALDIARGEKKDLVLISVQPKPIARILDYGKFKYDRKKKEKEQKEKQTNINNRQIRLTPLIGDHDLLTKAKKTRELLLKGDRIKVSLKFKGREIARKELGIDTLNRFYEQVEDIAKIDKEPKLNQDRFLDMYLHPDKQKIAKYLKEKGEDNAKNEK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins. The designed protein sequence is ",
    "response#1": "VQVKEIKFRPGTDEGDYQIKLRNILRFLEDGDKVKITVRYRGREMAHQEIGLDVLERVKTDTSEVAIVESAPGKLEGRQAVMVIAPS",
    "response#2": "VQVKEIKFRPGTDEGDYQIKLRNILRFLEDGDKVKITVRYRGREMAHQEIGLDVLERVKTDTSEVAIVESAPGKLEGRQAVMVIAPV",
    "response#3": "RIMNYGKFIYEKEN"
  },
  {
    "reference": "MTDQKLVTVIDYGMGNLRSVAKAAEHAADSNTRICISDKAEDIRSADAIIFPGQGAAKACMKALNETNITHALIQAATEKPFLGICMGLQVLMTHSQENEGVDCLDILKGDVQKFDLSGHPELKMPHMGWNQIHQTIDHPLWHNIEQNSRFYFVHSYFVSPHDKQIIAGETTHGKRFTSAIAQNNLFAIQAHPEKSADAGLQLFKNFLNWNGQ",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should interact with enzymes and substrates within the Amino-acid biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "EVIHTLDNPYSEHGGLSMLHGNIAPLGGVIKVGGVDPSIQTFTGAAICFDSHDAAVAAIDNHTVRPGHVVVIRYEGPKGGPGMPEMLAPTSSIVGRGLGKEVALITDGRFSGATRGIAVGHISPEAAALGPIALIEDGDMVTIDLVHRTLNVDVTDDELKQRKARLQPFKAKVKSGYLARYTALVTSANTG",
    "response#2": "EVIHTLDNPYSEHGGLSMLHGNIAPLGGVIKVGGVDPSIQTFTGAAICFDSHDAAVAAIDNHTVRPGHVVVIRYEGPKGGPGMPEMLAPTSSIVGRGLGKEVALITDGRFSGATRGIAVGHISPEAAALGPIALIEDGDMVTIDLVHRTLNVDVTDDELKQRKARLQPFKAKVKSGYLARYTALVTSANTG",
    "response#3": "ESPVELTERLSDRLGVNFYVKREDRQRVFSFKLRGPYNMMSSLSHEEIDKGVITASAGNHAQGVPFPFPGRRLKCVAKIVMPTTTPNIKLDGVRALGADVVLWGHTFDEAKTHAVELCEKDGLRTIPPFEDPAVIKGQGTIGSEINRQIKRIDAVFVPVGGGGLIAGVAAFFKQIAPQTKIIVVEPYDAASMALSVHAEHRAKLSNVDTFADGATVAVIGEYTFARCQDVVDAMVLVANDGIGAAIKDVFDEGRNIVETSGAAGIAGMYCEMYRIKNDNMVGIVSG"
  },
  {
    "reference": "MESYLVDTYQGIPYTAAVQVDLVEKDLLPASLTIWFPLFQANTPPAVLLDQLKTLTITTLYAASQSGPILKVNASAQGAAMSVLPKKFEVNATVALDEYSKLEFDKLTVCEVKTVYLTTMKPYGMVSKFVSSAKPVGKKTHDLIALCDFMDLEKNTPVTIPAFIKSVSIKESESATVEAAISSEADQALTQAKIAPYAGLIMIMTMNNPKGIFKKLGAGTQVIVELGAYVQAESISKICKTWSHQGTRYVLKSR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: During virus assembly and budding, acts as a bridge between the nucleocapsid and the lipid bilayer.; Later in infection, traffics to the cytoplasm through the action of host CRM1 to associate with inclusion bodies, the site of viral transcription and replication; Plays a crucial role in virus assembly into filaments and budding.2. The protein design should include a Nuclear export signal motif to ensure proper function.3. The protein should be optimized for membrane, viral envelope, host cell cytoplasm-specific folding and stability, while maintaining structural constituent of virion. The designed protein sequence is ",
    "response#1": "LNPTHDIIALCEFENIVTSKKVIIPTYLRSISVRNKDLNTLENITTTEFKNAITNAKIIPYSGLLLVITVTDNKGAFKYIKPQSQFIVDLGAYLEKESIYYVTTNWKHTATRFAII",
    "response#2": "LNPTHDIIALCEFENIVTSKKVIIPTYLRSISVRNKDLNTLENITTTEFKNAITNAKIIPYSGLLLVITVTDNKGAFKYIKPQSQFIVDLGAYLEKESIYYVTTNWKHTATRFAIA",
    "response#3": "LNPTHDIIALCEFENIVTSKKVIIPTYLRSISVRNKDLNTLENITTTEFKNAITNAKIIPYSGLLLVITVTDNKGAFKYIKPQSQFIVDLGAYLEKESIYYVTTNWKHTATRFAIR"
  },
  {
    "reference": "MQNVFYSLRELDKRAEEDFNLKNGILMENAARGSAEKIKNLFPLKKGEAKKTLQIVCGSGDNGGDGLALARILADDFNIHVIILKEPKSELCKLQYERLKALNIRTAKNILPESDILFDAFLGTGLKGFLKAEDKKTIEKINKVKAFNIACDIPSGLNLDGEASPNAVLCNMTFSMGALKQAFYSDEAKDITGKIEVIDLGLPRSSYEQNEKSNIFLLEKEDMLLPSRKKQNTHKGSFGHAGIIMGEKNGAALLAASAALEFGAGLVTLIGENSERPKSLKADFMYDGKFEAGKFNVKKFTSIAIGQGLGRKNDGLFSILKVKETQSMPMVIDADIFYYAELKKILPKLSAAVLTPHPKEFSSLLNIAGFGSLSIEEIQKKRFILALSFSKKFPKLVLVLKGANTLIAHNGKICINTLGSPSLSKAGSGDVLTGLICSLLAQGYKPLDAAITGSLAHSLASQNAGASYSLTASKLIKNLEKLEAEQFKESKARQVK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The AMP, (6S)-NADPHX, K(+) binding site should be located in a region of the protein that is accessible to the ligand.2. The designed protein should have ATP binding, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, NADHX epimerase activity, metal ion binding to facilitate nicotinamide nucleotide metabolic process.3. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.4. The designed protein must have at least one YjeF N-terminal domain.5. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "MMITDINCECLGLSRLCLMESAGKSLAEEVGKIAVYTFSKPVKIAIFTGSGGNGGDGFVAARYLLNRGYDVDIYMLTDKDNIHSDNAKTNFEIITNMEPRLSHLTVHYLDTIEDVNNCDIAQSESFSEFIIVDAILGTGIKGELRPKVKRAVEVINKSNGLTVSVDVPSGMDPLTGEVSDVAVEPHYTISFHRVKTGIHLAGEDKVGGIVTCDIGIPIEAERFTADGTCLPKREKTAHKGLFGHVLVAGGERGFSGAVQMAASAALRVGAGLVSLATRSEHAALLNLVRPELMVHGIDKRVDLAPLLKKSSVAAVGPGLGQTVWAQNLLAELFDTAMPLVVDADALNLLAKEPFKRDNWVLTPHPGEAARLLGITIKEIESDRFSSVKALQKKYGGVVVLKGAGTLIFDGSEMVINTTGNPGMASGGMGDVLSGVIAALLAQGLSLSQSARLGVWLHGKAGDIAAKEGERGLLASDLLRPLRVLVNG",
    "response#2": "MGYGDLLRLKNRPSKSHKGNNGKVLIVGGSKDYSGAPTIAGLSAISSGADLVYVAAPESASLAIKSTSPDLIVRSLEGDYLTSQHAEEILELVEEVDAVLLGPGASLNDETAKLFNILATKIKKPLVIDADALKLVDINLIKNNENVILTPHLFEFKTFFADKIKELAIDESKLKLSLAGTEFSSIDENISMFQQITKAIKGTVIIKGEYDLILSGNKFKINKTGNPGMTVGGTGDALSGLATSLLSQGLDTFNAAALAIYINGRAGDVAMNKQGYGFSATDLIGYIGAIMVQGTTGLPGIAPRRPTIHKGSIGLVLALGGNLGMPGAIRMAAESALRAGAALVAVGCHQDNQAIVVQGRPELMLAPSEPERLVASSYTDKAKVILIGPGLGRDAWANQLVQAAIKAEKPMVVDADALIAMKALGARSDNWVLTPHPGEAAFLLDCDIAAIEADRFAAVRAIAEQFGGVCVLKGAGSLICDGQQVIVNTTGNAGMASGGMGDVLSGIIAALILQTGSLMDATRLAVSIHGQAAEHVTQNTGMRGLLASDLFEPIRQLVNQV",
    "response#3": "MRQADLECIQSGISADTLMENAGKQVALEVRKIPGEVFNKNIIVLAGPGNNGGDGLVAARYLDGWEAKVHLFLPGERSENDPNLKLVNQSRILVYKSIDQLGNLLRDADIVIDALFGTGSNRPIEGVYKEALAKVTEAKQKHYRFKVVAIDLPSGMNADSGYCDPSCLKADYTVTLGYPKIGLFTGKGPEKTGTVIVADIGIPDYMVPDVHKGKMGHVLLVGSSVGKTGALIMSAKAATRTGSGLVSVGVPKGLNQIFEIALTEEMSIPLEGEDRLSEKSADRIIQMQDSFSAIGVGMGMGTYQEGESIVKKLIKDIKKPLLLDADAINNLANIKDLNILKEREYPTVLTPHVGEFARLSGYEKDSIVHNLIDVAQEFSQKWNCFLVLKSSRTVISTPDGYAFVSLRGSPAMAKGGSGDVLSGILTSLMGRGIPLLDALRLGVFLHGLAGEIAERKRHTESVRALDIVEEIK"
  },
  {
    "reference": "MTNIGVVGATGQVGTVMRQLLEERNFPADSVRFFASARSAGKTLPFRGEEIVVEDTATADVSDLDIALFSAGATMSRVQAPRFAEAGVTVIDNSSAWRKDPAIPLVVSEVNPHDIPAGESARGRGIIANPNCTTMAAMPVLKPLHDAAGLVRLTVSSYQAVSGSGLAGVEELHSQALATVEKADALTHDGHAISFPDPVKYVAPIAFNVIALAGSIVDDGSFETDEEQKLRNESRKILGLPDLRVAGTCVRVPVFTGHSLSIHAEFAHDITPERATEILAGAPGVKLADVPTPLDAAGRDESLVGRIRQDRSLDGDKGLVLFVSGDNLRKGAALNTIQIAELLV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be optimized for activity in L-threonine from L-aspartate: step 2/5 pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. A protein with NAD binding functions is required to enable threonine biosynthetic process.3. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.. The designed protein sequence is ",
    "response#1": "LGALHHEYGLRELVVASYQAVSGAGQVGIDRLHAELAAVAGKQVGARAGDVREALAAAGLSQEDSPFSGPVVLNVVPWAGSLKDDGWSSEELKVRNESRKILGIPDLKVSATCVRVPVVTTHALAVHATFERPVTVAEAHKIFDAQPTVVLVDDASDGRYPMPADVVGEDPTYVGRVRQALDFPNTLDFFVCGDNLRELLP",
    "response#2": "LGGLFRENLVEWATSMTYQAASGAGAQNMRELLQQMGEAHRVAGDLLRDPASAILDIDREVAGTLRDERFPTAHFGVPLAGSLIPWIDKDLGNGQSKEEWKGGAETNKILGREANPTPIDGLCVRIGAMRCHSQALTIKLTRDVPLDEIDAIIASANSWVKVVPNEREASMHELTPTAVTGTLAVPVGRLRKMNMGSEYLSAFTVGDQLLL",
    "response#3": "LGGLFRENLVEWATSMTYQAASGAGAQNMRELLQQMGEAHRVAGDLLRDPASAILDIDREVAGTLRDERFPTAHFGVPLAGSLIPWIDKDLGNGQSKEEWKGGAETNKILGREANPTPIDGLCVRIGAMRCHSQALTIKLTRDVPLDEIDAIIASANSWVKVVPNEREASMHELTPTAVTGTLAVPVGRLRKMNMGSEYLSAFTVGDQLLVK"
  },
  {
    "reference": "MADRPIRVRYAPSPTGHLHIGNARTALFNYLFARHNNGTLVLRIEDTDTKRNVADGEKSQMENLHWLGIDWDEGPDKGGDFGPYRQSERKDIYDKLIKQLVDKGFAYESYRTEEELKADREAQKARHEMPHYEYEYEGMTDAEKADAIAAAKAKGLEPVIRFHIPMDKTYEWDDIVKGKISIDANTLGGDFVIQKRDGMPTYNFAVVVDDHMMQISHVLRGDDHIANTPKQLAIYDAFGWEPPIFGHMTLIINGATGKKLSKRDETVLQFIEQYRELGYLPDAMFNFITLLGWSPVGEDEIFTKKEFIKQFDPKRLSKSPARFDQKKLEWVNNQYIKAKQKTNPNELMSLSLANLIEDGKIHSDPDPKTIEWARQLISLYTDQMSYTAQISKLADIFFDKATDLTDDERKELADDNAKPVIEAFAKKIRALDTFDAYSIGGIVNEVKQETKVKGRKLYMPIRIAATLRMHGPQLAETIELMGRDQVLKNVDLVTGQL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein have enhanced ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-   glutamyl-tRNA(Glu).2. The designed protein must possess glutamate-tRNA ligase activity to support glutamyl-tRNA aminoacylation under certain conditions. The designed protein sequence is ",
    "response#1": "SLKGSYVIAKYLARTTPSLSLYGENALSASKIDEFIDKFAHLKSEKFNEFLKEMNEYLTLRAFLIGFNLTLADIVLFARIKMVKEIQEEINKLGKTIPHLNRWYGYLSQLESFVEADNAFNGKKETKR",
    "response#2": "SLKGSYVIAKYLARTTPSLSLYGENALSASKIDEFIDKFAHLKSEKFNEFLKEMNEYLTLRAFLIGFNLTLADIVLFARIKMVKEIQEEINKLGKTIPHLNRWYGYLSQLESFVEADNAFNGKKETKC",
    "response#3": "SLKGSYVIAKYLARTTPSLSLYGENALSASKIDEFIDKFAHLKSEKFNEFLKEMNEYLTLRAFLIGFNLTLADIVLFARIKMVKEIQEEINKLGKTIPHLNRWYGYLSQLESFVEADNAFNGKKETKN"
  },
  {
    "reference": "MIQLKDISLSYPGHPAPVQALQGVNLQVRAGEVFGVIGRSGAGKSSLIRVINLLNRPSQGQVHVAGAELTALSASALRAARHQIGMIFQHFNLLSSRTVFDNVALPLELSGVRPALIRARVDELLDLVGLSALRERYPAQISGGQKQRVGIARALASKPKVLLSDEATSALDPETTRSILGLLRQINRDFGLTIVLITHQMQVIKQVADRVAVIDAGRIVEQGAVIDVFTRPQHATTRSLIDEILPQELPLSVIARIRALMAAEPQRSGQLLRLAFAGDGSGQPLLSDVIRRFGLDLNILHGQIDDIQGQTFGSLAVHVRGASAQLVQAVAHLRSAGVVVQEEEIAHV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit ATP binding. The designed protein sequence is ",
    "response#1": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYY",
    "response#2": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#3": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR"
  },
  {
    "reference": "MRVMGTQRNWPQWWIWGILGFLMLMICRGVGNSWVTVYYGVPVWTEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQELVLENVTENFNMWENDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNASNVSITSTDNKTKLEGEIKNCTFSTTTEIKDKKQQAYALFYRSDLVPLHENNSSNGSDFILIHCNTSTITQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGTGPCQNVSSVQCTHGIKPVVSTQLLLNGSLAEGDIIIRSENLTNNVKTIIVHLNESVEIKCVRPGTNTRRRRIGIGPGQAVFTNNMIGDIRQAHCSINASKWSSTIEKVRTKLKEHFPNRTITFKPSSGGDLEITTHSFNCGGEFFYCNTTKLFNDSISNATNLNISNTTDNITLPCRIKQIINMWQEVGRAMYAPPIEGNITCASNITGLLLLRDGGNTSDTNGTEIFRPGGGNMKDNWRSELYKYKVVEIKPLGIAPTEAKRRVVEREKRAVLGALLLGFLGAAGSTMGAASITLTVQARNLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLSDQQLLGLWGCSGKLICTTNVPWNSTWSSKTQAEIWNNMTWMQWDREINNYTGIIYTLLEESQIQQDKNEKELLALDSWKNLWSWFDISNWLWYIRIVIMIVGGLIGLRIIFGVLSIVKRVRQGYSPLSLQTLTPDPRELDRLGRTEEGGGEPGKDRSIRLLNGFLAVAWEDLRSLCLFSYHLLR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that can perform structural molecule activity in plasma membrane, virion membrane to support fusion of virus membrane with host endosome membrane, mitigation of host antiviral defense response.2. The protein must exhibit the following characteristics: During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state.3. The designed protein should contain one or more Retroviral envelope protein GP41-like domains that are essential for its function. The designed protein sequence is ",
    "response#1": "LWVTIYYGVPVWKEANTTLFCASDAKAYKTEVHNVWATHACVPTDPNPQEVVLENVTEYFNMWKNNMVEQMHEDIINLWDQSLKPCVKITPLCVTLSCTNLRNDTNTTRNGTNSTINSEVRGEMTNCSFNVTTTVRGRVQKEYALFYRLDVVPIEDSNNTEFRLINCNSSTITQTCPKVSFEPIPIHYCAPAGFAILKCNDKKFNGTGQCTNVSTIQCTHGIRPVVSTQLLLNGSLAEEEVVIRSENFTENTKNIIVQLNESVQINCTRPNNNTRKSIQLGPGAAFYATGDIIGDIRQAHCNLSEEQWNKTLKQVVTKLKEQFNKTIVFTQPSGGDPEIVMHSFNCGGEFFYCNTSQLFNSTWYSNSTWDNSSTGNTTNKNITLPCRIKQIINMWQAVGKAIYAPPISGQISCSSNITGLILTRDGGKNTTNDTFRPAGGNMKDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRXXXXGE",
    "response#2": "MRVKGIRKNCQSLLRGGTLLLGMLMICSATEDLWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQEVVLENVTENFNMWKNDMVEQMHKDIISLWDQSLKPCVKLTPLCVTMNCSNVSRSNENTTTITTSTVSTTMSNDTYEIDMKNCSFNMTTELRDKKKNVHALFYNLDIVQISNSSEYRLVKCNTSVISQACPKISFEPIPIHYCAPAGYAILKCNNKTFNGTGPCQNVSTVQCTHGIMPVVSTQLLLNGSLAEEGIIIKSENLTNNVKTIIVHLNESVDIVCVRPHNNTRQSIRIGPGQTFYATGRITGDIRRAHCNINSTQWHRTVQKVKKKLEEKFPNNPIVFNSSSGGDPEIITHTFNCRGEFFYCDTSRLFNDTHWNDTEDNIANSNTIIPCKIRQIINMWQEVGRAIYAPPIEGNITCKSRITGLLLERDGGKKNDPKKIEIFRPGGGDMRNNWRNELYKYKVVEIKPLGVAPTEAKRRVVEREKRAVGLGAVFLGFLGAAGSTMGAASITLTAQARQLMSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLALERYLKDQQLLGIWGCSGKLICTTAVPWNASWNNRSLDDIWNNMTWMQWEREIDNYTHEIYNLISESQAQQEKNEQELLQLDKWASLWNWFDITNWLWYIKIFIMIVGGLIGLRIVFSVLAIVNRVRKGYSPLSFQTRLPAPRGPDRPEGIEEEGGE",
    "response#3": "MRVMGTQRNCPLLWKWGGILLLGILMICNAKENLWVTVYYGVPAWRDADTTLFCASDAKAHSTEVHNIWATHACVPTDPNPQEIYLENVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTSANLTKVNNATTSNDFNPIIGNITDEVKNCSFNMTTELRDKKHKVYALFYSLDIVQIDNSSEYRLIKCNTSVIKQACPKISFDPIPIHYCTPAGYAILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENITNNVKTIIVHLNKSVEINCTRPSNNTRTSIAIGPGQLFYRTGDIIGDIRAAYCEINGTKWNKVLKQVTEKLKEHFKEKNITFQQPSGGDLEVITHHFNCRGEFFYCNTTRLFNNTCINNETIEGCQNITIPCKIKQIINMWQETGQAMYAPPIRGKISCISNITGILLTRDGGANKTNEEIFRPGGGNIRDNWRSELYKYKVVQIEPLGVAPTRAKRRVVEREKRAALGMGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNASWSNKSQKEIWENMTWLQWDKEISNYTDIIYHLIKESQNQQEKNEQDLLALDKWADLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSVINRVRQGYSPLSFRLIPSPQGLDRP"
  },
  {
    "reference": "MVLKDKRILLGVCGGIAVYKSTMLARAMIKAGALVQVVMTDAATKFVGPLTFEALTERACLTDENLFHTGGGVSHVEVARNADLIVIAPATATTIARLAGGFADNMLSASVLASKAPILLVPAMHTAMWESVPVQRNLTLLNPQQYRILPPAAGDLASGDVGPGRFPEVEDIFDAVCAVASPQDLAGWRIAVTAGPTREPMDPVRFISNPSTGKMGIEIARAAQIRGAVVDLVLGPTHLNPPSELATGSMNVRKVLTTKDMLDSTKAALAGADALIMAAAPSDDRPAQVHEHKLHKADLPKTLQMEANPDILKTLRPDFDKKVIVGFAAETTSVVESGQQKLVDKGLDLLFANPVDNGQGFGAAANQGYLLSKDGALVVVESQSKFDVANLILDQVLKIGKRK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.2. The designed protein should contain one or more DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains that are essential for its function.3. The designed protein should have phosphopantothenate--cysteine ligase activity, FMN binding, metal ion binding to facilitate pantothenate catabolic process.4. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A5. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis pathway to avoid interference with native metabolic processes.6. The CTP binding site should be located in a region of the protein that is accessible to the ligand.7. The desired protein should be able to perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2 The designed protein sequence is ",
    "response#1": "MNIVLGVGGGIAAYKSALLLRLFTEAGHAVTVIPTQASTKFVGVATWEALSGRPVSNDVFHAVDQVNHVRLGHEADLIVVAPATADLLARAAGGHADDLLTTTLLMARGPVLFAPAMHTEMWQHAATQANVAALRSRGVHVLDPAVGRLTGPDTGPGRLPEPGLLADPAAVARAARVVLGVGAGIAAYKVCELLRRFTESGHDVTVVPTADALRFVGEPTWAALSGRPVATRVWDGVADVPHVRIGQTADLVFVAPATADLLARAAHGLADDLLTNTLLTARCPVVFAPAMHTEMWEHPATQANVATLRARGSIVVDPASGRLTGKDTGPGRLPDPEALFAAARQLLAPALDGRHVVVSAGGTREALDPVRFLGNRSSGLQGYALARTAAARGARVTLVAANVALPDPAGVRVVVVESALQLREAVLSAADRADAVVMAAAVADFRPAGYRPSKIKKRDGASAPSIELVQNPDVLAELGARPHAGQVIVGFAAETDDVLANGRAKLARKGCDLLVVNQVGADLAFGRPDNAAVVLGADGTETPVPHGPKDRLAAAVLDLVTPLLS",
    "response#2": "MNIVLGVGGGIAAYKSALLLRLFTEAGHAVTVIPTQASTKFVGVATWEALSGRPVSNDVFHAVDQVNHVRLGHEADLIVVAPATADLLARAAGGHADDLLTTTLLMARGPVLFAPAMHTEMWQHAATQANVAALRSRGVHVLDPAVGRLTGPDTGPGRLPEPGAVLADPGRIRVVLGVGAGIAAYKVCELLRRFTESGHDVTVVPTADALRFVGEPTWAALSGRPVATRVWDGVADVPHVRIGQTADLVFVAPATADLLARAAHGLADDLLTNTLLTARCPVVFAPAMHTEMWEHPATQANVATLRARGSIVVDPASGRLTGKDTGPGRLPDPEALFAVARELLAPRRDLDGRHVVVSAGGTREALDPVRFLGNRSSGLQGYALARTAAARGARVTLVAANVALPDPAGVRVVVVESALQLREAVLSAADRADAVVMAAAVADFRPAGYRPSKIKKRDGASAPSIELVQNPDVLAELGARPHAGQVIVGFAAETDDVLANGRAKLARKGCDLLVVNQVGADLAFGRPDNAAVLLAADGTESAVAAGSKAEVAGAVLDVAAGLLARRGE",
    "response#3": "MNIVLGVGGGIAAYKSALLLRLFTEAGHAVTVIPTQASTKFVGVATWEALSGRPVSNDVFHAVDQVNHVRLGHEADLIVVAPATADLLARAAGGHADDLLTTTLLMARGPVLFAPAMHTEMWQHAATQANVAALRSRGVHVLDPAVGRLTGPDTGPGRLPEPGRLADPGLLALAARAVLAPAELSGKRVLITAGGTREPLDPVRFLGNKSSGKQGVALAEAALAAGADVTVIHAHMDVAAPAGAKLQSVETALELRAATLSAAGTFDVVIMAAAVADFRPASISSGKIKKRDDTADPVINLVRNPDILVEVVAHRAAQNLKQLIVGFAAETGDDQDDALSYAEAKLQRKGCDLLVVNEVGPGDSGTERVFGQDSNQVQVLGADGSVEELPLLDKTEVADRVLDAVTALVRT"
  },
  {
    "reference": "MKAIAIGSINMDFTMSVDHLPVKGETIAAKSFKESAGGKGANQAVALSRLGADVMMIGAVGRDGMGSILMNSLKRDGIETKGIKEVDAPTGNAFITVDVDGNNTIVVYPGANYELDIGWVEAFKEDIRASDFVILQMEIPIDTVVGSIELANRLGTNVILNPAPAKALPDHIYRMIDMIIPNETEIALLTGIEDVKQGARRLLEKGVKRVVVTLGEKGCLYVDAERELMMEGNKVRAVDSTAAGDAFTAGLAISFGRGDDLKSALKFANAVGALTVTRVGAQDSLPTYKEVEMFLRRQEK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have metal ion binding, ATP binding, ribokinase activity within cytoplasm to regulate D-ribose catabolic process, D-tagatose 6-phosphate catabolic process.2. The designed protein should contain one or more Carbohydrate kinase PfkB domains that are essential for its function.3. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate, K(+), ATP binding.4. The protein must exhibit the following characteristics: The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.. The designed protein sequence is ",
    "response#1": "MNTVTVIGSINLDRTIRVENMPKPGETIHTKEIFSAGGGKGANQAVAAQRSGANTHFIGAVGDDAAGKTMLDLLTQEKINLAGITKMTNQSTGQAYVTVDDAGENQIMIHGGANMAFTPADVEAHRDIIETSDFVVAQFESAVDSTVEAFKIAQAAGVRTILNPAPAMEKVPAELLAVTDMIVPNETETETLTGIAITDEASMLKASAALHALGISAVIITIGSKGAFYDIDGRHGIVPAFKVEAVDTTSAGDTFIGAMSSVLNKDFSNLEDAIRYGNRASSIAVQRFGAQPSIRANRW",
    "response#2": "MKKILVLGSLNMDFVIRVKKMPEVGETILGEDVALVPGGKGANQAYAAGKLGGHVTMLGAVGMDPYGSSLTKNLQDAGVDTEGILRSKNDATGQAFITVYENGDNSIIVIPGANGRLKPEMLQESMKYIDACDYIIMQLEIPLETVRYVKELALRRGKKVVIDPAPAAAGLEDSFWHGITILKPNETELGILTGKQLRNETEMIEAARSLVFKGVETVLVTLGEKGCLMVREKEAVHFPAKKAEMVDTTAAGDSFLAALVTALCEDRPFEDAVEFAQTVSSIVVGRHGAQTSIPSWDSFL",
    "response#3": "MKKICVVGSINVDMVTSMDRFPKPGETVPGKEFNTFPGGKGANQAVAARKLGGNVKILGCVGSDSFGREMIDMFNNLGIDTSALKFEDSVSTGTATILVSGNGQNSIIVTPGANNYVTSDYVEKNIDTIRESDILMVQLEIPVETVIYAIKKATELKKLVIFDPAPVRPLPDDIFPYIDIITPNETELEGLTCMNIKSEKDRKAAVQILLEKGVKTVINKAGSKGAYIFTKNGFKHVPTYEVDAVDTTAAGDTFNAGLAVGISMDKSIEESMVIANAAAAISITKMGAQSAMPC"
  },
  {
    "reference": "MDTPLLNFRKVHFVTSAPDIRHLPNDGGIEIAFAGRSNAGKSSALNALTQQRQLARTSKTPGRTQLINLFELEPGKRLVDLPGYGYAQVPIEMKLKWQAALSEYLQKRESLKALVLLMDIRHPLKDLDLQMLEWASNSDLNILVLLTKADKLNPGPRKTVVQQVRKATMVFGDSVRVEAFSSLKGTGVDEVTRILSDWYQPEDTPDEISEAE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Necessary for normal cell division and for the maintenance of normal septation..2. The designed protein should contain one or more EngB-type G domains that are essential for its function.3. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "VGVEIAFAGRSNAGKSSALNALTEQKNLARTSKTPGRTQLINVFELDAQRRLVDLPGYGFAQVPLAMKMKWQQSLAEYLQKRACLSGVVVLMDIRHPLKDLDMQMIEWAVASEIPVLALLTKSDKLAQSAKMKTVNEVRKALAEFGDWVQVESFSALKGTGKPKVLSILNEWCHR",
    "response#2": "EIAFVGRSNAGKSTCINTLTQQHRLAFASKTPGRTQSINLFSLGKQGVTDAVLADLPGYGYAAVPKEAKYRWQQVMGNYLQTRDNLKAVVLLCDPRLGLTELDEILLDVLRPRVEEGLKFLVLLD",
    "response#3": "ELPEIALAGRSNVGKSSFINTMLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYLTTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESAIKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKA"
  },
  {
    "reference": "MRVMETQRNCQHLLRWGIMILGMIIICSTTENLWVTVYYGVPVWKDTETTLFCASDAKAYETEKHNVWATHACVPTDPNPQEIYLENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLNCSNVRVNASSIETNASRSNVSSINGXSLEEIRNCSFNMTTELRDKKKQVYALFYKLDVVQINENESNSSKYRLINCNTSAITQACPKVTFEPIPIHYCAPAGFAILKCRDEEFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAKNTVKIRSENITNNVKTIIAQLVNPVTINCTRPSNNTRTSIRIGPGQAFYATGDIIGDIRKAYCNVSESEWEKALKQVAGQLGKHFSNKTIKFTNSSGGDLEITTHSFNCGGEFFYCNTSGLFNSFWSYSNGTYSTNSTKSNGTNSTKSNGTIQLPCRIKQIINMWQRAGQAMXAPPIRGIIRXESNITGLILTRDGGSNGNNSNTTEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVWREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVRQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNKSQTEIWNNMTWLQWDKEITNYTQEIYKLIEESQNQQEKNEQDLLALDKWASLWNWFEISNWLWYIKIFIMIVGGLIGLRIIFAVLSVINRVRQGYSPLSFQTHTPNPGGLDRPGRIEEEGGEQGRDRSIRLVSG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should contain one or more Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains that are essential for its function.2. For general function, the protein need meet that During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Transmembrane protein gp41: Acts as a class I viral fusion protein; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane The designed protein sequence is ",
    "response#1": "MRVRGIQRNWQQWWIWSILGFWMLMICNGEGNLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQELALENVTENFNMWENDMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDAEVTGKTNTTIGEWEKVKEGEMKNCSFDAINTKNKVQKQYALFDTLDVVPIDDDNNSNSNYSDFRLTKCDTSVIRQACPKVSFEPIPIHYCAPAGFAILKCNETDFNGTGLCNNVSTVQCTHGIRPVVSTQLLLNGSLAEEGVVLRSKDFKENTKIIIVQLNKAVNITCTRPNNNTRKGVHMGPGGALFATDVIGDIRKAHCNITREEWNNTLKQIVLKLKEKFENKTKIVFTNSSGGDPEVTMHTFNCGGEFFYCNTTELFSSTWNITGDSIGNITGEYTLNITLPCRIKQIINMWQGVGKAMYAPPISGQIRCISNITGLLLTRDGGGNNTENDNNTEIFRPWGGDMRDNWRSELYKYKVVKLEPLGLAPTKAKRRVVQREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGMWGCSGKLICTTAVPWNRTWSNKTQDDIWNNMTWMQWEREINNYTDTIYSLLEESQNQQEKNEKDLLALDSWNNLWSWFSITNWLWYIRIFIMIVGGLIGLRIIFAVISIVNRVRQGYSPLSFQTLTHQQREQPD",
    "response#2": "MRVMGIQRNCQPWWIWSILGFWMLMICNVVGNLWVTVYYGVPVWRDAETTLFCASDAKAYDTEVHNVWATHACVPTNPDPQEIALINVTEYFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLNCTNYKNKTQTVGEIKNCSFNITTSIRDRVTKEHALFYSLDIVSLDNTSYTLISCNTSTITQACPKVSFEPIPIHYCAPAGFAILKCNNKTFNGSGPCTNVSTVQCTHGIRPVVTTQLLLNGSLAEEEVVIRSKNFSNNANTIIVQLNESVVINCTRPNNNTRRGIHIGPGGAFYTTGDIIGDIRKAHCSISREKWNNTLKQVVKKLKEQFNTTTIIFKPSSGGDPEIVMHSFNCGGEFFYCNTTSLFNSTWDDSTNSTGEGENITLPEDITLPCRIKQFINMWQKVGKAMYAPPIRGQIKCSSNITGLLLTRDGSGGNSTTTNETFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRAVGLGAMFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGMWGCSGKLICTTAVPWNRTWSNKTQDDIWNNMTWMQWEREINNYTDTIYSLLEESQNQQEKNEKDLLALDSWNNLWSWFSITNWLWYIRIFIMIVGGLIGLRIIFAVISIVNRVRQGYSPLSFQTLTQTPNPGDLDR",
    "response#3": "MRVKGIRKNYQHLWRWGTMLLGMLMICSAAGNLWVTVYYGVPVWKEAKTTLFCASDAKAYDKEVHNVWATHACVPTDPSPQEMFLANVTENFNMWENDMVDQMHQDIISLWDESLKPCVKLTPLCVTLNCRNANLNSTRKSGEIKNCSFNITTSIRDRVTKEHALFYSLDIVSLDNTSYTLISCNTSTITQACPKVSFEPIPIHYCAPAGFAILKCNNKTFNGSGPCTNVSTVQCTHGIRPVVTTQLLLNGSLAEEEVVIRSKNFSNNANTIIVQLNESVVINCTRPNNNTRRGIHIGPGGAFYTTGDIIGDIRKAHCSISREKWNNTLKQVVKKLKEQFNTTTIIFKPSSGGDPEIVMHSFNCGGEFFYCNTTSLFNSTWDDSTNSTGEGENITLPEDITLPCRIKQFINMWQKVGKAMYAPPIRGQIKCSSNITGLLLTRDGSGGNSTTTNETFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRAVGIGALFLGFLGAAGSTMGAASMTLTVQARLLLSGIVQQQNNLLRAIEAQQRMLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVSWNTSWSNKSLNEIWNNMTWMEWEREIDNYTSLIYTLIEESQNQQEKNEQELLELDKWASLWNWFDITNWLWYIKIFIMIVGGLVGLRIVFTVLSIVNRVRQGYSPLSFQTRFPVPRGPDRPEGIEEEGGERDRDRSTRLVTGFL"
  },
  {
    "reference": "MKILFLSANEFEDVELIYPYHRLKEEGHEVYIASFEKGVITGKHGYSVKVDLTFDEVNPDEFDALVLPGGRAPERVRLNEKAVEIARKMFTEGKPVATICHGPQILISAGVLKGRKGTSYIGIRDDMINAGVEWIDREVVVDGNWVSSRHPGDLYAWMREFVKLLK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should be able to enhance the efficiency of proteolysis.2. The protein must exhibit the following characteristics: Also displays proteolytic activity; Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage ; Thus, was shown to afford full protection against glycation of thioredoxin by glyoxal; Deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins and reactive carbonyl groups of glyoxals (Probable); Prevents acrylamide formation in asparagine/glyoxal and asparagine/sugar mixtures, likely by degrading asparagine/glyoxal Maillard adducts formed at high temperatures.3. The designed protein must have at least one PfpI endopeptidase domain.4. The Nucleophile of the protein must be engineered to enable effective catalysis of H2O + S-(1-hydroxy-2-oxoethyl)-L-cysteinyl-[protein] =   glycolate + H(+) + L-cysteinyl-[protein]. The designed protein sequence is ",
    "response#1": "MKPYLAAALQMTSRPNLTENLQEAEELIDLAVRQGAELVGLPENFAFLGNETEKLEQATAIATATEKFLQTMAQRFQVTILAGGFPFPVAGEAGKAYNTATLIAPNGQELARYHKVHLFDVNVPDGNTYWESATVMAGQKYPPVYHSDSFGNLGLSICYDVRFPELYRYLSRQGADVLFVPAAFTAYTGKDHWQVLLQARAIENTCYVIAPAQTGCHYERRHTHGHAMIIDPWGVILADAGEKPGLAIAEINWKTYQLVYFLDKILQKSPLPPGNVKKLGDTYPSISNARNAELRLRWGQIVLKNDHQEDFWKVKEFLHNQGKQKYTLPLYHAMMGGSEVAQTLAKETFASTASQLHSNVVNYVQQLL",
    "response#2": "MKPYLAAALQMTSRPNLTENLQEAEELIDLAVRQGAELVGLPENFAFLGNETEKLEQATAIATATEKFLQTMAQRFQVTILAGGFPFPVAGEAGKAYNTATLIAPNGQELARYHKVHLFDVNVPDGNTYWESATVMAGQKYPPVYHSDSFGNLGLSICYDVRFPELYRYLSRQGADVLFVPAAFTAYTGKDHWQVLLQARAIENTCYVIAPAQTGCHYERRHTHGHAMIIDPWGVILADAGEKPGLAIAEINL",
    "response#3": "MKPYLAAALQMTSRPNLTENLQEAEELIDLAVRQGAELVGLPENFAFLGNETEKLEQATAIATATEKFLQTMAQRFQVTILAGGFPFPVAGEAGKAYNTATLIAPNGQELARYHKVHLFDVNVPDGNTYWESATVMAGQKYPPVYHSDSFGNLGLSICYDVRFPELYRYLSRQGADVLFVPAAFTAYTGKDHWQVLLQARAIENTCYVIAPAQTGCHYERRHTHGHAMIIDPWGVILADAGEKPGLAIAEINSLMKPAEELAQLWAAEELDMKAIEAVAISPWKTYQLVYFLDKILQKSPLPPGNVKKLGDTYPSISNARNAELRLRWGQIVLKNDHQEDFWKVKEFLHNQGKQKYTLPLYHAMMGGSEVAQTLAKETFASTASQLHSNVVNYVQQISR"
  },
  {
    "reference": "MNSYAILLSLFFSFERLLTLANANSLYSPFNNSSFVDSDTSFSDLSRNGLLSLLDSNTTSASVQTIAISQTDNAASCIPSASLLSSSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSSVACFPYATSDAPNPIPRGDSATSTSIAPTYSASDSSATTITSSSPSTSIIGTGSTDTSVSSTLTYHTPIASPTTSSNSDNEYTVDVITSSSLSSFVITNVDSTTTSVINYIGASTLESSSLTNTVSPTESTFYETKSSTSSVPTQTIDSSSFTSSTPVSLTSSSTSSSGSSQDSTTIDSTPSTIATSTLQPTTSSPITTSAPSLSSALPTTYPSSLSTEVEVEYFTKTITDTSSIVTYSTGVETLYETETITSSEISSIIYNFSTPISGSSFPDGFKPINPTSFPSLTSSTKKIPSTTLPTSSKMITTTTPSVSNNTQSSFLIISTFTSSYEHSEPFKVSSVPLTSNNFSSISHSSASSLPITPSSYLSNTTLHSSVQSSQSSQFTVSVPSSTQSYSTSSNFTTPITISTSLSSFPTTIVSSSFQYSSLSSNVTTTNAQSSSLSSSNSSALTHISSSIVSSGSSSALSSSTIVSSINSSSSVFISSVSSSLQYSSSYVTETTTSGSVGFTTTIATPVGSTAGTVVVDIPTPSWVTETVTSGSVGFTTTIATPVGSTAGTVLVDIPTPSWVTETVTSGSVEFTTTIATPVGTTAGTVVVDIPTPSWVTETVTSGSVGFTTTIATPIGTTAGTVLVDIPTPSWVTETVT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that P cell-type specific protein which involved in agglutination during conjugation.2. The designed protein must be able to regulate conjugation with cellular fusion.3. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "VQPGGSMKLSCVASGFTFSNYWMNWVRQSPEKGLEWVAEIRLKSGYATHYAESVKGRFTISRDDSKSSVYLQMNNLRAEDTGIYYCTRPGVPDYWGQGTTLTV",
    "response#2": "VQPGGSMKLSCVASGFTFSNYWMNWVRQSPEKGLEWVAEIRLKSGYATHYAESVKGRFTISRDDSKSSVYLQMNNLRAEDTGIYYCTRPGVPDYWGQGTTLTI",
    "response#3": "LQGDSSLQVEISDAVSERDKVKFTVQTKSCLPHFAQTEFSVVRQHEEFIWLHDAYVENEEYAGLIIPPAPPRPDFEASREKLQKLGEGDSSVTREEFAKMKQELEAEYLAIFKKTVAMHEVFLQRLAAHPTLRRDHNFFVFLEYGQDLL"
  },
  {
    "reference": "MSPRNVIIVGSTGAVGQELLRILEQREFPLKNLKLTASAKSRGRTVKFRGETLKVEELKADSFAGQDIGLFSAGGAVSKEFAPVAVKAGCVVVDNTSAWRMDPDVPLVVPEVNAKAIAGHRGIIANPNCSTIGLVVVLKPLHDKARCRRCVVTTFQSVSGAGARALEEMIEESKAVLAGKEYRRTIFPHPIAFNCIPQIGQKKETFQPSGYTDEETKMIQETKKILEDDTVRVAPTCVRVPVMRCHSESVNLEFSKPISADRAREILRSAPGVRVVDDPSKMEYPLATMVAGKDESFVGRIRQDPTVEYGLHLWIVSDNIRKGAALNAVQIAELL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Acyl-thioester intermediate, Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.2. The protein contains novel Semialdehyde dehydrogenase NAD-binding domains that confer a unique function or activity.3. The designed protein should be optimized for NAD binding activity in order to improve threonine biosynthetic process, lysine biosynthetic process via diaminopimelate, isoleucine biosynthetic process, 'de novo' L-methionine biosynthetic process, diaminopimelate biosynthetic process.4. Ideal protein should have a binding affinity for key enzymes in the L-threonine biosynthesis pathway to enhance the overall efficiency of the pathway.5. The NADP(+), phosphate, substrate binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "NVAVVGATGSVGREMLNILAERQFPIKEIAALASGRSQGDIIDFGEDGRTLKVQNLEHFDFSGYDIALFAAGSGPAAQYAPIAAAAGCVVIDNSSLFRMERDVPLIVPEVNPTAIDGLKPINDAVGIKRVNVSTYQSVSGAGRLSMELLTVQSRALLNGGEVPPQSNQEKSVAFNAVPKIDRLMDNGYTKEEMKMVWETRKILEDPDILVNPTCVRVPVYLGHSEALHIETREKLSVGEARDLLDAAPGVVVLDGENPGDYPTATTEAANRDAVYVGRIREDVSHPRGINLWVVADNLRNAKTRNIIANPNCSTIQMVVALGPLHERYKIKRVVVSTYQSVTGSGKGAVDQMMNEREGKDGDMVYPHKIDMNVLPHIDVFQENLYTKEEMKMVNETVKIFGDENVKVTATCVRIPTMGGHSESVNLEFEEEYDLEEVTSILSKAPGVIVEDDPHNNVYPMPLNSHGKDEVFVGRIRRDETQPKSLNMWIVADNLRKGAALNAVQIAEQLIAKGLLTKA",
    "response#2": "LGGLFEKDLVEWVSVATYQAASGAGAKNMRELLVQMGELRDVVKTDLANPASSILEIERAVADKMRGDLPIENFGAPLAGSLIPWIDKLLDSGQTKEEWKGYAETNKILGLSDSPIPIDGLCVRIGALRCHSQAFTIKMKKDLPLAEIEQILAAHNEWVKVIPNDKEATLRELTPTKVTGTLSVPVGRLRKLNMGPEYLAAFTVGDQLLTTHGFELGGLFEKDLVEWVSVATYQAASGAGAKNMRELLVQMGELRDVVKTDLANPASSILEIERAVADKMRGDLPIENFGAPLAGSLIPWIDKLLDSGQTKEEWKGYAETNKILGLSDSPIPIDGLCVRIGALRCHSQAFTIKMKKDLPLAEIEQILAAHNEWVKVIPNDKEATLRELTPTKVTGTLSVPVGRLRKLNMGPEYLAAFTVGDQLLTTHGFEQYTLGGLFEKDLVEWVSVATYQAASGAGAKNMRELLVQMGELRDVVKTDLANPASSILEIERAVADKMRGDLPIENFGAPLAGSLIPWIDKLLDSGQTKEEWKGYAETNKILGLSDSPIPIDGLCVRIGALRCHSQAFTIKMKKDLPLAEIEQILAAHNEWVKVIPNDKEATLRELTPTKVTGTLSVPVGRLRKLNMGPEYLAAFTVGDQLLTTHGFEQYTLGGLFEKDLVEWVSVATYQAASGAGAKNMRELLVQMGELRDVVKTDLANPASSILEIERAVADKMRGDLPIENFGAPLAGSLIPWIDKLLDSGQTKEEWKGYAETNKILGLSDSPIPIDGLCVRIGALRCHSQAFTIKMKKDLPLAEIEQILAAHNEWVKVIPNDKEATLRELTPTKVTGTLSVPVGRLRKLNMGPEYLAAFTVGDQLL",
    "response#3": "LGGLFEKDLVEWVSVATYQAASGAGAKNMRELLVQMGELRDVVKTDLANPASSILEIERAVADKMRGDLPIENFGAPLAGSLIPWIDKLLDSGQTKEEWKGYAETNKILGLSDSPIPIDGLCVRIGALRCHSQAFTIKMKKDLPLAEIEQILAAHNEWVKVIPNDKEATLRELTPTKVTGTLSVPVGRLRKLNMGPEYLAAFTVGDQLLTLKPIHDVSRIKRIVVATYQAVSGTGKRAIEELSNQTQAIFNLKEIVCNVYPHQIAFNCLPHIDIFLENGYTKEEMKMCYETKKIMEDDSIEVTATCVRVPVFYSHSEAVNIELEKRLTANEVRDILSNAPGVKVADNPDKNEYPLAIYAAGRDEVFVGRIREDASKENCVDMWIVSDNIRLKPINDAVGIKRVNVSTYQSVSGAGRLSMELLTVQSRALLNGGEVPPQSNQEKSVAFNAVPKIDRLMDNGYTKEEMKMVWETRKILEDPDILVNPTCVRVPVYLGHSEALHIETREKLSVGEARDLLDAAPGVVVLDGENPGDYPTATTEAANRDAVYVGRIREDVSHPRGINLWVVADNLRESKTVNSLGGLFEKDLVEWVSVATYQAASGAGAKNMRELLVQMGELRDVVKTDLANPASSILEIERAVADKMRGDLPIENFGAPLAGSLIPWIDKLLDSGQTKEEWKGYAETNKILGLSDSPIPIDGLCVRIGALRCHSQAFTIKMKKDLPLAEIEQILAAHNEWVKVIPNDKEATLRELTPTKVTGTLSVPVGRLRKLNMGPEYLAAFTVGDQLL"
  },
  {
    "reference": "MRLLLLVPLLLAPAPGSSAPKVRRQSDTWGPWSQWSPCSRTCGGGVSFRERPCYSQRRDGGSSCVGPARSHRSCRTESCPDGARDFRAEQCAEFDGAEFQGRRYRWLPYYSAPNKCELNCIPKGENFYYKHREAVVDGTPCEPGKRDVCVDGSCRVVGCDHELDSSKQEDKCLRCGGDGTTCYPVAGTFDANDLSRGYNQILIVPMGATSILIDEAAASRNFLAVKNVRGEYYLNGHWTIEAARALPAASTILHYERGAEGDLAPERLHARGPTSEPLVIELISQEPNPGVHYEYHLPLRRPSPGFSWSHGSWSDCSAECGGGHQSRLVFCTIDHEAYPDHMCQRQPRPADRRSCNLHPCPETKRWKAGPWAPCSASCGGGSQSRSVYCISSDGAGIQEAVEEAECAGLPGKPPAIQACNLQRCAAWSPEPWGECSVSCGVGVRKRSVTCRGERGSLLHTAACSLEDRPPLTEPCVHEDCPLLSDQAWHVGTWGLCSKSCSSGTRRRQVICAIGPPSHCGSLQHSKPVDVEPCNTQPCHLPQEVPSMQDVHTPASNPWMPLGPQESPASDSRGQWWAAQEHPSARGDHRGERGDPRGDQGTHLSALGPAPSLQQPPYQQPLRSGSGPHDCRHSPHGCCPDGHTASLGPQWQGCPGAPCQQSRYGCCPDRVSVAEGPHHAGCTKSYGGDSTGGMPRSRAVASTVHNTHQPQAQQNEPSECRGSQFGCCYDNVATAAGPLGEGCVGQPSHAYPVRCLLPSAHGSCADWAA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be involved in extracellular matrix organization, negative regulation of peptidase activity by localizing to extracellular region, extracellular matrix and exhibiting metalloendopeptidase activity, serine-type endopeptidase inhibitor activity.2. The designed protein's functionality will benefit from Polar residues, Basic and acidic residues compositional bias in its structure.3. The designed protein have Ig-like C2-type, TSP type-1, BPTI/Kunitz inhibitor domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "PNICDGDFDTVAMLRGEMFVFKGRWFWRVRHNRVLDNYPMPIGHFWRGLPGDISAAYERQDGRFVFFKGDRYWLFREANLEPGYPQPLTSYGLGIPYDRIDTAIWWEPTGHTFFFQEDRYWRFNEETQRGDPGYPKPISVWQGIPASPKGAFLSNDAAYTYFYKGTKYWKFDNERLRMEPGYPKSILRDFMGC",
    "response#2": "TGRKWNNHHLTFSIQNYTEKLGWYHSMEAVRRAFRVWEQATPLVFQEVPYEDIRLRRQKEADIMVLFASGFHGDSSPFDGTGGFLAHAYFPGPGLGGDTHFDADEPWTFSSTDLHGNNLFLVAVHELGHALGLEHSSNPNAIMAPFYQWKDVDNFKLPEDDLRGIQQLYGC",
    "response#3": "VFCYSPKDEGLCSANVTRYYFNPRHKACEAFNYTGCGGNDNNFVNLKDCKRTCG"
  },
  {
    "reference": "MILKELLKDIEYNLIQGDLNREVDSIAYDSRQIKSKGLFVCIEGYVSDGHNYIPDALTKGATSLIVNKEVKNINSDITVIQVEDTRKALAYIASAFYGYPANKITLTGITGTNGKTTTTYLIGRILEEYNYKVGILGTIENRIGKTVMKTERTTPESLELQALFSQMLKEDVSHCIMEVSSHALELSRVAGCFFDIAIFTNLTLDHLDFHKNMENYKRAKAKLFKNCKYAIVNIDDANAKDIIKESSGKIITFAIENEADFRAYDIKISSKGIEFSVKIDNEEVRFKLNTIGRFNVYNALGAIAAAYYLKVPTEIIKKALENMGGVPGRVQNVGSSLDFDVLVDYAHAPDGLENVLKTIKEFVKGEIITVFGCGGDRDKSKRPIMGKIAGDYSDYCIITSDNPRSEEPEKIIEEIEIGIKETNCSYEKIVDRKEAIKRAIKRAKKDDIVLIAGKGHENYQVLKDKVIHFDDGEVVKAALQEDMNGSN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have a high specificity for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate, ATP binding, with minimal off-target effects.2. The protein should be designed to catalyze the following reaction ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.3. The protein design must feature the Meso-diaminopimelate recognition motif motif.4. The ideal protein would be one that plays a critical role in cell wall organization, regulation of cell shape, peptidoglycan biosynthetic process, cell cycle through ATP binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, magnesium ion binding within cytoplasm.5. Ideal protein should have a binding affinity for key enzymes in the peptidoglycan biosynthesis pathway to enhance the overall efficiency of the pathway.6. The protein must bind to Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MKLQQLLGLLPDAIEISAIEFDSRKVQKGHLFVAIPGTQVDGHQYIEKAIDLGASAILCERLPETLKEQVTYIQVENSAKAMGLAAANFYGHPSRRLQLVGITGTNGKTSVATLLFNLFRKLGYKCGLLSTVQNQIEDEIIDSTHTTPDSVSINKLLQEMVNKGCGFVFMEVSSHAVVMERIAGLHFAGGIFTNITQDHLDFHETFKNYIAAKKGFFDQLPKTAFALTNTDDKNGKVMLQNTKATKESYSLRGVGTFKGKILDSGLFGIQMTIDEQEVWFKLIGRFNAYNLLAVYGAASVLLGLPWEAMTLDSRTAAAGDLFIAVLGHKTDGRRYIAQAVAQGVAAVIAEAEGEASDGCIREVHGVPVVYLSQLNRKLSALAGRFGHEAEQEVIGPLTIPVPGRVGRMQRLGGRLQNDEPLVVIDYAHTPDALEKTLEALRPMAQARGGELVCMFGCGGDRDATKRPLMGAIAEKLADGVVVTSDNPRSEEPAAIIDQIVAGMQDASKVRRIEDRASAILQAIRHAAREDVIVLAGKGWEAYQQVRQEWGL",
    "response#2": "MKLKDILLRTEVVKLQGNIEMEIKGISMDSRKTLPGDIFICMKGIKADGHEFVADAVAKGAVAIMAEEPVAAPVPVLYVNDTSRALAMTASAFYGITGTNGKTTSTFLMEAMLNAAGRKTALIGTICNKIGNEIIPSFLTTPGLLDLFAFARHALEAGCTDLVMEASSHALDQGRVAGISFKTALFSNLTQDHLDYHLTMDLYFEAKKTLFTKYLSKEGHAVINVDDAYGKILFDSLDGNKMAVSRMKSKEAMIYPEGNVAQSERGMTLLVPAISNQPFETHLCGDFNVDNVLLVLIKGISMDSRKTLPGDIFICMKGIKADGHEFVADAVAKGAVAIMAEEPVAAPVPVLYVNDTSRALAMTASAFYNIKLEDIIIGLSNFNGPEGRFQQIHSADGKVGIVDYAHTPDALQNVLETINDLREDIQQVITVVGCGGNRDKGKRPQMAKIAAELSHKVIMTSDNPRNEEPEAILKDMQEGISITKRKKVLSIVDRREAIKTAVMLANEGDIILVAGKGHETYQIFKNKTIHFDDREIAREALNI",
    "response#3": "MRLADLAGGLGLDGAGDADVLQTDSRRVSPGDGFIAWPGAATDGRAHVGDALARGAAACLVEQEGAGIDGLTADSRAVSRGFLFAALEGVKSDGADFIPQAEENGAAAILARPGVRSTLPVIEDPEPRRRLAQMAARFYPRPVKVMAMIGITGTNGKTTVATLVWNILTELGHEAGVIGTIENRIGDRSFASERTTPESVDLQEMMAEMAAAGVTACVMEVSSHALAQNRVAGTDFEVAAFTNLSQDHLDFHGSMEDYFAAKGRLFTDYLRPGGKAVINADDPWGRRLAANTKAEILTYGLDADADLRAENVQVSADGTRFTLATAGGAVEVQLKLPGRFNVYNALGAIGAALHLGISLDAVAAGLAACRGVPAGRMEVFSAPGRPTVLVDYAHTPDALEKALEAARLHCHGQLWCVFGCGGDRDRGKRPLMGALAEQLADRVVITDDNPRTEDPQAIVADILAGLVAPGVAQVIAGRAEAVTAAVMNAAPNDVVLLAGKGHETYQIMGDRTIHFDDREEAARALAARG"
  },
  {
    "reference": "MIKDLKSLTWNELEQLAAQYGQKSFVVRYLFTFIHQKNVSSLEQITPLSKTFRAALTEAGYSISSLKQIKTFEDLDGTIKFVFETPDGNRLEAVRLLDDGRNTLCISTQIGCRMGCLFCATGNLGYQRNLTCGEIVDQVYQIQARVGKVQNIVYMGMGEPLDNYQATMRSVELLHHSDGQNMGIRHITISTCGLTEGITRLAKEPLLPRLAISLHGPDDQTRQCLMRISKKVSIRELMSALRNYQDQTGRRITFEYCMIQNMNDSPQQARLLARLIKPFKCNVNLIEFNAFDGCDFTAASPAGIRQFAAILEREGIETVIRYKRGRSIRAACGQLGADWLKPAT",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be able to perform rRNA (adenine-C2-)-methyltransferase activity, rRNA binding, metal ion binding under specific conditions.2. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.3. The designed protein should contain one or more Radical SAM core domains that are essential for its function.4. The presence of [4Fe-4S] cluster is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "TTQVGCKMSCKFCASGLIKTERNLSVDEIVKQIYTMNLYLKEKYKNDPDNKRARVSHIVVMGIGEPFDNYENVLNFVKIINDKRGFEIGARHITISTCGIVPKIKEFADLKTQINLAISLHASNNDVRNQIMPINKAYPIEKLLDSVRYYVDQTNRRVTFEYILIDGINDKPML",
    "response#2": "TTQVGCKMSCKFCASGLIKTERNLSVDEIVKQIYTMNLYLKEKYKNDPDNKRARVSHIVVMGIGEPFDNYENVLNFVKIINDKRGFEIGARHITISTCGIVPKIKEFADLKTQINLAISLHASNNDVRNQIMPINKAYPIEKLLDSVRYYVDQTNRRVTFEYILIDGINDKPG",
    "response#3": "SSQVGCRQGCAFCASTIGGLVRCLEPSEILDEVLFSQIDSGKQISNIVLMGIGEPLDNFDNVVRFLKLVNDPDGMNIGMRHISLSTCGITERFDELADLNLQLTLSVSLHAPDDETRSKIMPANRGRGVDELMTCCKRYYEKTGRRISFEYIMIDGVNDTPC"
  },
  {
    "reference": "MRVKGTQRNWQNLWRWGLMIFGILMICSAKEEENLWVTVYYGVPVWKEATTTLFCASDAKAHETEVHNVWATHACVPTDPNPQEIIMGNVTENFNMWTNNMVEQMHEDIISLWDESLKPCVKLTPLCVTLTCTEAKNNGTNNATTNNNVTVNDPMKGEVQNCSFNVTTAINDKKQQIHALFYRVDMVSINETIGTYRLINCNTSVIKQACPKVSFEPIPIHYCAPAGFAILKCNNKNFTGTGPCTNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENITDNFKNIIVHLNESVPIVCTRPSNNTIEGVSIGPGQVFYRTGDIIGDIRQAYCNISAELWNTTLERIATKLGKHINMSTIIFRPASGGDMEVTTHTFNCRGEFFYCNTTPLFHSTWTKNDSDSHSWMKNDTIILPCKIKQIIRMWQRTGQAMYAPPIRGAIECKSNITGLILTRDSPGNNSGSNNNETFRPGGGDMRDNWRSELYKYKVVQIEPLGIAPTRAKRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQFLGIWGCSGKIICTTAVPWNMSWSPNKTYDDIWNNMTWIEWEREISNYTEQIYGLLTKSQDQQEKNEKDLLALDKWASLWNWFEISRWLWYIKIFQMIIGGLIGLRIVFAVLSIINRVRQGYSPLSFQTLTPHPADLDRPRGIEEGGGEQDRDRSIRLVNGFLALAWDDLRSLC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should exhibit Polar residues compositional bias.2. The designed protein must have at least one Human immunodeficiency virus 1 envelope glycoprotein Gp120 domain.3. A protein that can perform structural molecule activity in host cell endosome membrane, host cell plasma membrane, viral envelope to support mitigation of host antiviral defense response, positive regulation of establishment of T cell polarity.4. The protein design must feature the Di-leucine internalization motif, YXXL motif; contains endocytosis signal motif.5. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWKEATTTLFCASDAKSYKAEAHNIWATHACVPTDPNPHEIKLENVTENFNMWKNNMVEQMHEDIISLWDQSLTPCVKLTPLCVTLNCTEWKGNATNGNTTKMNETTQEDTGVKYCSFNVTTELRDRKKRVHALFYKLDVVPIDDSNISYRLINCNTSTITQACPKISFEPIPIHYCAPAGFAILKCNNRKFNGTGPCRNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTDNAKIIIVQLNESVTINCTRPNNNTRKSTRIGPGASLYTTDIIGDIRQAHCNISRERWNKTLQQVAKKLRDLLNTTTIKFKPHSGGDPEITTHMFNCGGEFFYCNTSGLFNSTWGKDTNSTRVENNSTITLRCNIKQIINMWQGVGKAMYAPPIKGVISCSSNITGLLLTRDGGVKNNSEETVRPGGGDMRDNWRSELYKYKVVKIEPLGLAPTRAKRRVVEREKR",
    "response#2": "MGAASLTLTVQARQVLAGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKHICTTAVPWNSSWSNRSLEEIWGNMTWMEWEREIDNYTNLIYNLIEESQIQQEKNEQELLQLDKWASLWDWFTITHWLWYIKIFIMIVGGLIGLRIVFAVLSLVNRVRQGYSPLSFQTN",
    "response#3": "LWVTVYYGVPVWKEAKTTLFCASDAKAYDTEVHNVWATHACVPTDPSPQEMVLKNVTENFNMWDNDMVEQMHQDIISLWDQSLKPCVKLTPLCVTLXCSNVNRTSNNC"
  },
  {
    "reference": "MPMPLNQLLPQAEGSVLVRELTLDSRKVRPGDLFLAVPGGRQDGRAHIGDAIARGAVAVAYEAEGAPALQADGAILVPVKELADQLSAIAGRFYGEPSRALRLVGVTGTNGKTSVSQLVAQALDRLGERCGIIGTLGSGFHGELKSGLHTTPDPIGVQAALAQLKQAGARAVAMEVSSHGLDQGRVAALAFDVAAFTNLSRDHLDYHGSMEAYAAAKAKLFAWPELSCRVINLDDAFGRELAGRHHDSRLISYSLTDPAAYLFCRDAHFSDDGVRADLITPRGEGILRSSLLGRFNLSNLLAVVGCLLGMDYPLDEILRVMPDLQGPAGRVQRLGGGVAPLVVVDYAHTPDALEKVLEALRPHASGRLLCLFGCGGDRDRGKRPLMAAVAERLADAVLVTDDNPRSEDPAQIVADIRAGFSEPERVEFMHGRGQAIGHLIASAQVGDVVLLAGKGHEDYQEIDGVRQPFSDLNEASQALAAWEAAHA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The presence of Mur ligase N-terminal catalytic domains is critical for the interaction of the designed protein with its intended targets.2. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity. The designed protein sequence is ",
    "response#1": "VTGTNGKTTTTHLIEALLKQGDRATGLLGTLYARWPGHDTVAIHTTPFAVDLQADLAAARDAGCQALVMEVSSHALHQKRVWGCPFEVAVFTNLTQDHLDYHETMAAYFEAKALLFSEDYLQGRAIVNLDDPYGQQLIERLGDKAWSYSVENSQADLYTTDLTYGASGVQGQLHTPNGTIPFSLPLVGQFNLANMLAAVGALELG",
    "response#2": "VTGTNGKTSSVQWIAAALNNSEVPCGTIGTLGVTLPDGTNLGGKLTTPDVLTMHRSLAKLREAGANLVALEASSIGIVQGRLNGVHIAIAALTNLSHDHLDYHLTFENYQAAKAKLFQWPGLESMVINADDAFGQTVLATAAEARKISYSLQSREADVFGDDVHIGGDGQVFNLITSHGTAQILTHLLGEHNVANLLLVSGH",
    "response#3": "VTGTNGKTTTTHLLAQWTHLLGKVSAVMGTVGKGIIGQINNTAATNTTGSPVEIQQLLASLKNQGASLAAIEVSSHGLVQHRVSHLHFAAAVFTNLSIDHLDYHCDMVQYEAAKWRLFNELDVVQRIINADDATGRRWLEQLPQAVAVSISGDLPTLWKGNWLCADKVHYHAHGTEISFRSSWGTGLLYSQLVGEFNVNNLLLALALLDAVGYPFNRLMDTLAEFSGVRGRFELVDEGQDFPVIVDYAHTPDSLENVLKTVKQIADNKVFVVVGCGGDRDKTKRPLMAKIACKYATDPIFTSDNPRSEDPNAILADMIEGVKGKRYEALVDRKDAILKAIESAEKGDVVLIAGKGHEDYQEINGERLPFSDMEQASQALQRRMV"
  },
  {
    "reference": "MTNWGPTGELVYNRTYSRTKPDGSKETWPETVRRVVDGNLALVDERYHLPGERADLIRLMEEFKILPGGRHLWASGVKNAQHLFNCWVSGWTEKPSDHFEFTFMRLMEGGGVGANYSNRFIDYGPVQQELYVHIVCDPDHPDYEAMKEAGVLSTEYDPDWAGAFVIEDSREGWAAALVDLIDTHYRDEVSHFQRVYDVSRVRQFGAKLKTFGGTASGPLPLARMLIDVCEILSEIATEGGQLTGIAAMEIDHAIAQCVVAGGVRRSARMSMMHWKDPQVYEFLRIKQDTGSHWTTNISLEVDDEFWVAVEEGWAGPNNRILRELTEGMVANGEPGFWNSSLSNVGEPNEVVCTNPCGEITLEPWEPCNLGHVNLAAFAHGNGSYDITGLYRAHRLVTRFLMRATFSPVADPKSREVLDRNRRIGVGHLGVASFLALCGWKYSEARTNEEFKWLLRSLAEEVDHAATQFAHQLRIPVPVKKRTVAPTGTIAKMPGVSEGIHPIFSRYFIRRVRLSMSDPDQTRMLADYGRQGYEVEDDLYAKFTGVVSIPTQDTLVAEVIEHYGQDSESLVESAADLSLNELLGFQALYQTIWADNAVSFTANVDPETYNADDVRQQLRLFGGLLKGATIFPEASMPQAPYERITKEQYEQATAKAVADGVDEDCANGACPIR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein's active site must be carefully engineered to ensure that the amino acids involved in [thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-   triphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-   triphosphate are properly positioned for optimal catalysis.2. A protein with nucleotide binding, cobalamin binding, ribonucleoside-triphosphate reductase activity, ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor is needed to enhance DNA replication in a specific context.3. The stability of the protein-adenosylcob(III)alamin complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "ESGLDFFAYFFKILASQLVSCATPVMRSAGLRQSYLASCFIMNPDMSTEDKTLSAVFRDLSPLLCSKSGVGMNLTNFSSGGKNVQSCLRLINSQVEFCNDKNLRPVSVAAYMELWHEQIEEFLAAKLPENPERCQSIFQGVCVPGLFFRLYEQNPDSQWHLFSPKVGGHLAGLYGDKFDEEYARLVRHGLYSSSLPAKSLMFALISAIIKTGSPYILSKDAINRHHWFETQGNAISYANLCAEVVQQPHEFTSTCNLANVCLPACLRARGEGEGAGAAGEGEGADRLSPLTPDLEFCFDTLRAAVRAAVYMINASILGGVCPTPGVRVMQAERSAGIGVQGLADVFAKLGRGYMDAESALLDARIFEVMYYQAVRASNQLVTVGGAPPHANWKNSKLSRGEFHWESWGVQYDSLFIERQLWEELRESVTKHGTFNSQFIALMPTAGTSQLTGLSESFYPFYANVSSKVSNKEEVMKPNITFLERVSPSDVPLLKYHGGDVSKLPPPLAAKYRNFLTAFDYSPEDQIRRAAARSPFVDQSQSFSFFLKEANVKSASYVKNLILLGHGLGLKTIMYYCRIETQAAYLSGLFQTDGCVNANHKAKALTIELTSIHYESLQDVQKLLLNMGVYTTIYSNNKRLASCFILQPSMMNEPGTLDALYHDMSPLLASKSGVGLDVTSFSHQKNIASCLKLVDAQVHYFNDNNIRPVGASAYMELWHSQICDFLNAKLPENPDRCHSLFQGVCIPTLFFRMYEKDPSKLWYLFDPATAPNLIKLYGAAFDNEYERLVRAGKYVSCMPLKSMMFTLIHTIIKTGSPYVLLKEALNEHHWTDTQGMAINCSNLCAEIVQLPGRNTSVCNLANICLPKCLRTVESARVGTTDANRPFFCFEALGDAVRVAVLVINACILGGSHPTPGVERGQKERSMGIGVQGLADVFAELGYGYLDAESAELDKNIFQSMYYTAVETSHNLVLEGQGVPFHGWEVSNFAKGRFHWQTWEGEDASFVPRHRWDALGKSIAEHGIFNSQFLAVMPTAGTSQVTGYAESVYPFFANISSKVTNKEEVLRPNVTFFKKVLPDDLRVVRQYGGDVSTFPKHHRERYRVFLTAFDYCPFKLLDRARARAPFVDQSQSMSFFLKEDRVRNASYLRDLLLHGYRLGLKTLMY",
    "response#2": "ESGLDFFAYFFKILASQLVSCATPVMRSAGLRQSYLASCFIMNPDMSTEDKTLSAVFRDLSPLLCSKSGVGMNLTNFSSGGKNVQSCLRLINSQVEFCNDKNLRPVSVAAYMELWHEQIEEFLAAKLPENPERCQSIFQGVCVPGLFFRLYEQNPDSQWHLFSPKVGGHLAGLYGDKFDEEYARLVRHGLYSSSLPAKSLMFALISAIIKTGSPYILSKDAINRHHWFETQGNAISYANLCAEVVQQPHEFTSTCNLANVCLPACLRARGEGEGAGAAGEGEGADRLSPLTPDLEFCFDTLRAAVRAAVYMINASILGGVCPTPGVRVMQAERSAGIGVQGLADVFAKLGRGYMDAESALLDARIFEVMYYQAVRASNQLVTVGGAPPHANWKNSKLSRGEFHWESWGVQYDSLFIERQLWEELRESVTKHGTFNSQFIALMPTAGTSQLTGLSESFYPFYANVSSKVSNKEEVMKPNITFLERVSPSDVPLLKYHGGDVSKLPPPLAAKYRNFLTAFDYSPEDQIRRAAARSPFVDQSQSFSFFLKEANVKSASYVKNLILLGHGLGLKTIMYYCRIL",
    "response#3": "ESGLDFFAYFFKILASQLVSCATPVMRSAGLRQSYLASCFIMNPDMSTEDKTLSAVFRDLSPLLCSKSGVGMNLTNFSSGGKNVQSCLRLINSQVEFCNDKNLRPVSVAAYMELWHEQIEEFLAAKLPENPERCQSIFQGVCVPGLFFRLYEQNPDSQWHLFSPKVGGHLAGLYGDKFDEEYARLVRHGLYSSSLPAKSLMFALISAIIKTGSPYILSKDAINRHHWFETQGNAISYANLCAEVVQQPHEFTSTCNLANVCLPACLRARGEGEGAGAAGEGEGADRLSPLTPDLEFCFDTLRAAVRAAVYMINASILGGVCPTPGVRVMQAERSAGIGVQGLADVFAKLGRGYMDAESALLDARIFEVMYYQAVRASNQLVTVGGAPPHANWKNSKLSRGEFHWESWGVQYDSLFIERQLWEELRESVTKHGTFNSQFIALMPTAGTSQLTGLSESFYPFYANVSSKVSNKEEVMKPNITFLERVSPSDVPLLKYHGGDVSKLPPPLAAKYRNFLTAFDYSPEDQIRRAAARSPFVDQSQSFSFFLKEANVKSASYVKNLILLGHGLGLKTIMYYCRIL"
  },
  {
    "reference": "MGFSPKTLVAFCWTVLFIISFVHCLPMTHGGKPGYGIERTIIRPEWRCYVDEGCPKGQGAPCEKNCKNVADYGICIGIKCCCFDVIPVPPT",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a specific function within the extracellular region. The designed protein sequence is ",
    "response#1": "PGDGGPPGQPGPDGPPGAPGNAGEDGKPGDQGPRGPPGIPGAPGQPGRPGPPGEPGL",
    "response#2": "PGDGGPPGQPGPDGPPGAPGNAGEDGKPGDQGPRGPPGIPGAPGQPGRPGPPGEPL",
    "response#3": "PGDGGPPGQPGPDGPPGAPGNAGEDGKPGDQGPRGPPGIPGAPGQPGRPGPPGEPG"
  },
  {
    "reference": "MKIGSGEKLLFIGDSITDCGRARPEGEGSFGALGTGYVAYVVGLLQAVYPELGIRVVNKGISGNTVRDLKARWEEDVIAQKPDWVSIMIGINDVWRQYDLPFMKEKHVYLDEYEATLRSLVLETKPLVKGIILMTPFYIEGNEQDPMRRTMDQYGRVVKQIAEETNSLFVDTQAAFNEVLKTLYPAALAWDRVHPSVAGHMILARAFLREIGFEWVRSR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein with the reaction Deacetylation of xylans and xylo-oligosaccharides., the Nucleophile, Charge relay system must be optimized to ensure efficient catalysis.2. The ideal protein would be one that plays a critical role in xylan catabolic process through acetylxylan esterase activity within cytoplasm.3. The protein design should consider the endogenous proteins and enzymes present in the xylan degradation, Glycan degradation pathway to avoid interference with native metabolic processes.4. The target protein must exhibit strand-helix-turn-strand-helix-helix-helix-strand-helix-helix-strand-helix-helix-helix-helix as its primary conformation.5. For general function, the protein need meet that Acetylxylan esterase involved in the degradation of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose; Also hydrolyzes fully acetylated methyl-beta- D-xylopyranoside and methyl-beta-D-glucopyranoside, and the synthetic substrates 2-naphthyl acetate, 4-nitrophenyl acetate, 4- methylumbelliferyl acetate, and phenyl acetate.; Completely deacetylates xylobiose peracetate (fully acetylated), and is active on both the alpha- and beta-forms of the sugar The designed protein sequence is ",
    "response#1": "FFWWSEALHGAANMGNVTNFPEPVGMAASFNPHLLFKVFDIASTEFRAQYNHRMYDLNGEDMKMRSLSVWTPNVNIFRDPRWGRGQETYGEDPYLTSVMGVQVVKGLQGPEDARYRKLWACAKHYAVHSGPEYTRHTANLTDVSARDFWETYMPAFKTLVKDAKVREVMCAYQRLDDDPCCGSTRLLQQILRDEWGFEYLVVSDCGAVSDFYENHKSSSDAVHGTSKAVLAGTDVECGFNYAYKSLPEAVRKGLLSEKEVDKHVIRLKPSFLLGCAMNFRILQSEGYRNWFASRFKITSFTNEMKWYTTEKERGHENYTAADSMLKFAEENGILVRGHTVLWDDPLMQPTWVPKIEDPNDLMNVTLNRINSVMTRYKGKLTGWDVVNENVHWDYFEKMLGANASSSFYNLAFKLDPDVTMFVNEYNTIENRVEVTATPVKVKEKMEEILAYPGNMNIKGAIGAQGHFRPTQPNLAYMRSALDTLGSLGLPIWLTEVDMPKCPNQEVYIEEILREAGKKGLKGTFWNNTEMAGKPFTTEYYTKPVNVTTAGMHVFAPNLPIEDFSAKYETTFTAKEAGEYVVNVESTGHFELYVNGKQQFVNHIWRATPTRTVLKAEKGQKFDIEVRFQTVKTWGASMKIDTRVEAETIAWQAGVTTEPTQASGGPISNLNVTNIHNGDWIAVGKADFGSAGAKTFKANVATNVGGNIEVRLDSETGPLVGSLKVPSTGGMQTWREVETTINNATGVHNIYLVFTGSGSGNLLNLDAWQF",
    "response#2": "MKWYATEAVRGQENYKIADSMMQLAEENAILVKGHTVLWDDKYWQPNWVKTITDPEDLKNVTLNRMNSVMKRYKGRLIGWDVMNENVHFNYFENMLGGNASAIVYSLASKLDPDIPLFLNEFNTVEYDKDRVVSPVNVVKKMQEIVSFPGNNNIKGGIGAQGHFAPVQPNLAYMRYALDTLGSLSFPVWLTEVDMFKCPDQVKYMEDILREAYSHPAVKAIILYGGPEVSGFDKLTLADKDFKNTQAGDLIDKLGKKGLKGTFWNNTEMAGKPFTTEYYTKPVNVTTAGMHVFAPNLPIEDFSAKYETTFTAKEAGEYVVNVESTGHFELYVNGKQQFVNHIWRATPTRTVLKAEKGQKFDIEVRFQTVKTWGASMKIDGKKGLKGTFWNNTEMAGKPFTTEYYTKPVNVTTAGMHVFAPNLPIEDFSAKYETTFTAKEAGEYVVNVESTGHFELYVNGKQQFVNHIWRATPTRTVLKAEKGQKFDIEVRFQTVKTWGASMKIDDGKKGLKGTFWNNTEMAGKPFTTEYYTKPVNVTTAGMHVFAPNLPIEDFSAKYETTFTAKEAGEYVVNVESTGHFELYVNGKQQFVNHIWRATPTRTVLKAEKGQKFDIEVRFQTVKTWGASMKIDVARELNIDYQETIAQLT",
    "response#3": "MKWYTTEKERGHENYTAADSMLKFAEENGILVRGHTVLWDDPLMQPTWVPKIEDPNDLMNVTLNRINSVMTRYKGKLTGWDVVNENVHWDYFEKMLGANASSSFYNLAFKLDPDVTMFVNEYNTIENRVEVTATPVKVKEKMEEILAYPGNMNIKGAIGAQGHFRPTQPNLAYMRSALDTLGSLGLPIWLTEVDMPKCPNQEVYIEEILREAYSHPAVKGIIIFAGPEVSGFDKLTLADKYFNNTATGDVIDKGKKGLKGTFWNNTEMAGKPFTTEYYTKPVNVTTAGMHVFAPNLPIEDFSAKYETTFTAKEAGEYVVNVESTGHFELYVNGKQQFVNHIWRATPTRTVLKAEKGQKFDIEVRFQTVKTWGASMKIDDKIQCETMTKSGQYTGNISSPFNGVALYANNDAVKYTQYFASGTHDFTLRGCSNNNKMARVDLKIGGQNKGTFYYGDSYPAEYTIKNVSHGTGNQTIELVVTADDGQWDAYLDYFNNGKKGLKGTFWNNTEMAGKPFTTEYYTKPVNVTTAGMHVFAPNLPIEDFSAKYETTFTAKEAGEYVVNVESTGHFELYVNGKQQFVNHIWRATPTRTVLKAEKGQKFDIEVRFQTVKTWGASMKIDT"
  },
  {
    "reference": "MASSVLEMIKEEVTCPICLELLKEPVSTDCNHSFCRACITINYESNRNTEGEGSCPVCRVCYLFRNLRPNRHVANIVERLKGFKSIPEEEQKVNVCAQHGEKLQLFCKKDKMAICWLCERSQEHHGHKTALVEEVDHEYKEKLQDALQKLMEKEKTCDEWQDVIQQQRTYWENQIQSDIKNVQIEINGLRELLDSKENEELQELKKEKEDVFQRLEESENELVQQRESVRDCISDVEHQLELSTMEMLQSVEYVLRRSQTLKLKLPDIISERRRKFQAPDLKGMLQAFEGLMDIQRYWVHVTPCQYNKEIVSINKNKGQIQCASHYRRNRQVSETFHLGVVGYPAIQSGKHYWEVDVSTCDAWLLGLSDGKCAQPHLMGEMGFKMKLNSNFNQIEMYQPKCGYWVIGMKDRSVYNAFDECSITHNPSVLVLCLPHAPSRVGVFVDREACTLSFYDVSNSGALIYRFYYPSFPHRVFPYFNPLECLKPMTVCGPPS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein contains novel B box-type, B30.2/SPRY, RING-type domains that confer a unique function or activity.2. A protein that contributes to positive regulation of NF-kappaB transcription factor activity, negative regulation of viral entry into host cell, positive regulation of MAPK cascade, protein polyubiquitination, regulation of lipopolysaccharide-mediated signaling pathway by functioning in cytosol, nucleoplasm, P-body, cytoplasm with protein kinase binding, ubiquitin-protein transferase activity, protein homodimerization activity, ubiquitin protein ligase activity, protein-macromolecule adaptor activity. The designed protein sequence is ",
    "response#1": "ELRTVCRVPGLVETLRRFRGDVTLDPDTANPELILSEDRRSVQRGDLRQALPDSPERFDPGPCVLGQERFTSGRHYWEVEVGDRTSWALGVCRENVNRKEKGELSAGNGFWILVFLGSYYNSSERALAPLRDPPRRVGIFLDYEAGHLSFYSATDGSLLFIFPEIPFSGTLRPLFSPLSSSPTPMTICRPKGGR",
    "response#2": "LLKKFKEDLQGELEKEEKVELTLDPDTANPRLILSLDLKSVRLGQRAQDLPNHPRRFDTNTRVLASCGFSSGRHHWEVEVGSKDGWAFGVARESVRRKGLTPFTPEEGVWAMQLNNGQYWAVTSPERTQLNCGHLSRVRVALDLEVGAVSFYAVEDMRHLYTFRVNFQERVFPLFSVCSTGTYLRIWS",
    "response#3": "DWWVEDLAELSIDLFKRVVSTIRRKGGVLPEVIGEALEVYAAKRIPGFMIQNDDNDDEEDVMEQRSLLETLVSMLPSEKQSVSCGFLIKLLKSSVSFECGEEERKELSRRIGEKLEEANVGDLLIRAPEGGETVYDIDIVETLIDEFVTQTEKRDELDCSDDINDSSKANVAKLIDGYLAEISRIETNLSTTKFITIAEKVSTFPRQSHDGVYRAIDMFLKQHPGITKSEKKSSSKLMDCRKLSPEARSLLGVFEEDATAISNYMNQLYQAMHRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLANIGTSVDLRSICHVPGLKKMLRSYGVYITLDQDTANPWLILSEDRRQVRLGNTRQDVPENEERFDNYPIVLSAQSFNSGKHYWEVDVTGKEAWDLGVCRNSVRRKGQFLVDTENGFWTIWLWNKKYEAGTCPQTPLHLQVPPCQVGIFLNYEDGIISFYNITDHGSLIYTFSECAFTEPLRPFFNPGFNNGGGNSGPLALCPLKR"
  },
  {
    "reference": "MGWAGRARRQGGRPAPPPLLLLLQAALLLVAVPPGARAYNLDTGSPLVFQGPQGTLFGYSVLLHRHGEDRWLLAGAPTASWLSNSSVANPGAVYRCQIGDAPDRTPCEQLQVGSPAGESCGKTCSGERDNQWLGVSLSRQPKENGSIVACGHRWKNVFFVKNENKLPNGVCYRIPSDLRTELSQRLCPCYMDHIKKFGENYGSCQAGISTFYTEDLIIMGAPGAYYWTGTIFVYSTTANSFVAYSDASNQVKFGSYLGYSVGAGHFLSPRSTEIIGGAPQYEQIGKAYLFRRETKRLAIIQEMMGKKLGSYFGASVCAVDLNADGFSDLLVGAPMQSTVREEGRVFVYLNSGSDGNMIEMETELKGSDTYSARFGETIANLGDIDNDGFEDVAIGAPQEDELRGAIYIYNGRDNGISPSFSQRIEGHRIRNTLSMFGQSISGGIDADNNGYSDVAVGAFLSDSAVLLRTRPVVIVDASLNHSTSVNRTVLNCVESGQPSVCVDLNLCFEYKGRRVPGYIVLFYTVILDINRKAETPSRFYFSSNGSSEVTSGSIKVTSGKASCRTHQAFMRKDVRDILTPIHIEATYHLGEHIVGKRSSEGFPPLQPILQQRKEKDIIKSKIVFARFCGHENCSADLQVSANVGYPKPNDKKTYLVVGSMKTLMLNVSLYNAGDDAFGTALHIKLPKGLYFIKVLELEEKLINCEVTENSGLVALECTVGHLYVDHLSKMDFSFLLDASSLTRAEEDLNITINATCENEGEMDNISNN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have integrin binding, C-X3-C chemokine binding within integrin complex, integrin alpha4-beta7 complex to regulate leukocyte tethering or rolling, negative regulation of protein homodimerization activity, cell-cell adhesion mediated by integrin, integrin-mediated signaling pathway.2. The protein should have Helical domains to ensure proper insertion into the membrane.3. The protein must contain a signal peptide for proper functionality.4. The protein contains novel Integrin alpha-2 domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "AQPVVMATVQLMVQDSLNPTLKNCVLEQTKTPVSCFNVQMCVGATGHNIPQKLHLKAELQLDLQKPRQARRVLLLASRQASLTLSLDLGGRNKPICHTIKAFLRDEADFRDKLSPIVLSLNVSLPPEETGVAPAVVLHGVTHVQEQTRIILGGAETCEDCLLIGPQCAWCSQENFTHPSGVGERCDTPATLLAKGCQ",
    "response#2": "AQPVVMATVQLMVQDSLNPTLKNCVLEQTKTPVSCFNVQMCVGATGHNIPQKLHLKAELQLDLQKPRQARRVLLLASRQASLTLSLDLGGRNKPICHTIKAFLRDEADFRDKLSPIVLSLNVSLPPEETGVAPAVVLHGVTHVQEQTRIILK",
    "response#3": "TCEDCLLIGPQCAWCSQENFTHPSGVGERCDTPATLLAKGCQLTFIENPVSQVEILTNKPLSVGRQKNSSDIVQIAPQKLTLKLRPGSEQTLQVQVRQTEDYPVDLYYLMDLSASMDDDLNTIKELGSLLSKEMSKLTSNFRLGFGSFVEKPVSPFVKTTPEEIANPCSSIPYFCLPTFGFKHILPLTNDAERFNEIVKNQKISANIDTPEGGFDAIMQAAVCKEKIGWRNDSLHLLVFVSDADSHFGMDSKLAGIVIPNDGLCHLDNKNEYSMSTVLEYPTIGQLIDKLVQNNVLLIFAVTQEQVHLYENYAKLIPGATVGLLQKDSGNILQLIISAYEELRSEVELEVLGDTEGLNLSFTAICNNRALFPHQKKCSHIKVGDTVSTGSVNCRKYVQVSKKLSYCTEAGIFLSRISKVIFKNFLINAFGKADPHATLFSPMHFGGWKKIISIFFFQASFNVTVSIPNCERRSRHIIIKPVGLGDALEILVSPECNTL"
  },
  {
    "reference": "MAMLLNHLLPQTEGSILIRALTLDSRKVRPGDLFLAVPGAVHDGRQHIADAIARGAVAVAYEADGSAQQLDIPGALLVPVKGLARQLSALAGRFYGEPCRSLRLIGVTGTNGKTSVSHLIAQGLDLLGERCGIIGTLGTGFHGELEQGIYTTPDAIGVQESLARMKQAGARAVAMEVSSHGLEQGRVAALDFDVAVFTNLSRDHLDYHGTMQAYGEAKARLFEALDLGCRVVNLDDAFGRELARRKHASRLITYSLEDASATLFCPNPLLNDDGIQAQLITPQGKASLRSSLLGRFNLSNILAALGALLSMDYPLDEVLAVLPQLQGPIGRMQRLGGGNVPLVVVDYAHTPDALEKVLTALRPHTQGRLRCLFGCGGDRDRGKRPLMAGTVERLADTVLVTDDNPRNEDPERIRGDIRAGFEQPDRVEFVAGRAKAIAGLIADSHPTDVVVLAGKGHENYQEIQGMRSPFSDIDEARKALSAWEVGHA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein should be able to manipulate the activity of enzymes in the Cell wall biogenesis, peptidoglycan biosynthesis pathway, either by activating or inhibiting their function.2. The protein should have a high affinity for ATP, meso-2,6-diaminoheptanedioate.3. A protein that can perform ATP binding, magnesium ion binding in cytoplasm to support regulation of cell shape, peptidoglycan biosynthetic process, cell cycle.4. Incorporating a Meso-diaminopimelate recognition motif motif is critical for the successful design of the protein.5. The protein should be designed to have high stability and activity towards ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.6. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MKLGITDVKNDSRQIKPGDLFIAYPGAAADGRLFIEKAVSLGAAAVAYEPDNFPPSCILPSSVPCVAIPHLAEQLADIAKRFYGQPSRELQMIGVTGTNGKTTCCFLITQTLQKLNCKSALMGTLGYGMADKVLQTQNLTTPDAVTLHQNYAALLEEGAKVIAMEVSSHGLDQNRLNGLKIDSGIFTNLTQDHLDYHKDMNTYLKAKQKLFERPELKRAIINLDSPYFDKVLAKINPKLPIFIYSLNQPKLLRRSYPVSTSFIYAKHYSFHNNGIVAEVVTPWGEGELRSPLMGDFNLSNLLAVFGTAVALGYDVQSAISALQSAPQVPGRLEHVHDPAGRITALVDYAHTPDALRNVLETLRALLPDGHRLSCVVGAGGDRDRSKRPEMARIAAELADQVILTTDNPRSESPEAILDEMEAGVPPAAAPRVLRITDRRQAIRTAVRLGHEVLLVAGKGHEDYQIIGKTKHHFDDREQASKALDRRNSGNS",
    "response#2": "MKIRELTLDSRSVRPGDLFLAVPGAKVDGREHIADALARGAAAVAYEEQGANVLPLTDVPLIPVKGLIAQLSDIAGRFYGHPTDQMRVIGVTGTAGKTSVASFTRQIWAEAGHPAAQIGTTGVVSPSRNDYGSLTTPDPVALHKLLAELAAEGVTHAAMEASSHGLDQCRLDGVRLSAAAFTNLGRDHMDYHPTVEEYMSAKMRLFDTLLPKGAPAVIFADDPWSGEAIRVARAAGHDVRTVGRNGDFLALKRVEHLRHKQVAEVHFGDEIHEVHIPLAGDFQVANALVAAGLAMAAGAQPDQIFAALEELHGVAGRLEHVHDPAGRITALVDYAHTPDALRNVLETLRALLPDGHRLSCVVGAGGDRDRSKRPEMARIAAELADQVILTTDNPRSESPEAILDEMEAGVPPAAAPRVLRITDRRQAIRTAVRLGHEVLLVAGKGHETTQQIGDEVLPFDDAEVAREALAGKGGRA",
    "response#3": "MTRPILITDVKNDSRQIKPGDLFIAYPGAAADGRLFIEKAVSLGAAAVAYEPDNFPPSCILPSSVPCVAIPHLAEQLADIAKRFYNAPSQKLQVIGVTGTNGKTTCCFLITQTLQKLNCKSALMGTLGYGMADKVLQTQNLTTPDAVTLHQNYAALLEEGAKVIAMEVSSHGLDQNRLNGLKIDSGIFTNLTQDHLDYHKDMNTYLKAKQKLFERPELKRAIINLDSPYFDKVLAKINPKLPIFIYSLNQPKLLRRSYPVSTSFIYAKHYSFHNNGIVAEVVTPWGEGELRSPLMGDFNLSNLLAVATGISLAGHLVQRGDIYAALAGANRHGAEFSAQAIQRGAVAILTDERGAQLAAEDAAEAGIPLLLATNPRALTGEIAALGIPAADALPALAEVTPPPGRMEKVTPTQSTDVDSGLLAVVDYAHTPDAVEAALAALRPTTTGRLVVVLGAGGDRDRGKRHGMGRAAAGLADVVVVTDDNPRSEDPAQIRTAVVEGARSIDTTALLVEVGDRRKAIREGVRLAREGGSGSVLAVVGKGHEDYQIVGDEKRAPFDDRQVARDALAARKA"
  },
  {
    "reference": "MASGRRAPRTGLLELRAGAGSGAGGERWQRVLLSLAEDVLTVSPADGDPGPEPGAPREQEPAQLNGAAEPGAGPPQLPEALLLQRRRVTVRKADAGGLGISIKGGRENKMPILISKIFKGLAADQTEALFVGDAILSVNGEDLSSATHDEAVQVLKKTGKEVVLEVKYMKDVSPYFKNSTGGTSVGWDSPPASPLQRQPSSPGPTPRNFSEAKHMSLKMAYVSKRCTPNDPEPRYLEICSADGQDTLFLRAKDEASARSWATAIQAQVNTLTPRVKDELQALLAATSTAGSQDIKQIGWLTEQLPSGGTAPTLALLTEKELLLYLSLPETREALSRPARTAPLIATRLVHSGPSKGSVPYDAELSFALRTGTRHGVDTHLFSVESPQELAAWTRQLVDGCHRAAEGVQEVSTACTWNGRPCSLSVHIDKGFTLWAAEPGAARAVLLRQPFEKLQMSSDDGASLLFLDFGGAEGEIQLDLHSCPKTIVFIIHSFLSAKVTRLGLLA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Binds to phosphatidylinositol 4,5-bisphosphate .2. The designed protein must have at least one SU, PDZ, PH domain.3. A protein that can perform sodium channel regulator activity, transmembrane transporter binding in protein-containing complex to support ventricular cardiac muscle cell action potential, regulation of ventricular cardiac muscle cell membrane repolarization. The designed protein sequence is ",
    "response#1": "EGSIYHLASILFVVGFMGGSGFSGLLYVFSLLGLGFLCSSVWAWLDVCAADIFSWNFILFAICFVQFIYVTYQVRSVSFDKEFQELYSALFQPLGISLTVYRKIVLCCDAEVITLEKEHCYAMQGKTPIDKLSLLVSGRIRVTVDGEFLHYIFPLQFLDSPEWDSLRPTEEGIFQVTLTAETDCRYVAWRRKKLYLLFAKHRFISRLFSILIGSDIAEKLYALNR",
    "response#2": "PMYLSLEVSVGKATTIYAINGSAILLPCTFSSCYGFENLYFRWSYNNSETSRILIDGIVKNDKSDPKVRVKDDDRITLEGSTKEKMNNISILLSDLEFSDTGRYTCFVRNPKEKDLC",
    "response#3": "PMYLSLEVSVGKATTIYAINGSAILLPCTFSSCYGFENLYFRWSYNNSETSRILIDGIVKNDKSDPKVRVKDDDRITLEGSTKEKMNNISILLSDLEFSDTGRYTCFVRNPKEKDLC"
  },
  {
    "reference": "MKLDNVFKKTRLSSGRKGYISLSSARPEVPEGLLRKCNKCGSAIIAEDVKNDHYICPKCGGYFRVHAYRRIEMVTDAGTFEEWDTGLATPNPLEYKGYEEKLCALRAKTDLDEAVVTGKAQIDGIEVVIGVCDGRFLMASMGEVVGEKIARAVERASKERLPVILFACSGGARMQEGIISLMQMAKTSAALKRHSDAGLLYISVLTDPTTGGVTASFAMLGDVILAEPKALIGFAGPRVIEQTIGQKLPKGFQRSEFLLEHGFIDGIVERPQMKETLAQILMLHKQGNIKAEHSDDRDDADGNTGEARDQNEGIGKLSVNNQLASGWDRVQLSRMNDRPVGSDYIRELFTDFIEFHGDRYFADDKAVIGGIALFRGMPVTVIAQAKGTNTRENIERNFGMPSPDGYRKALRLMKQAEKFHRPVICFVDTPGAFCGLEAEERGQGEAIARNIYEMSGLRVPVLSVIIGEGGSGGALAMATADEVWMLENSIYSILSPEGFATILWKDSSKAKEAADVMRLTAEDLYRMGIVEEVLAEPSPYTMENLKEVTRVLESKLERFLSRYGKMSCDELTQRRYDRFRKL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein contains novel CoA carboxyltransferase C-terminal domains that confer a unique function or activity.2. Zn(2+) is required for the proper folding and stability of the protein.3. For general function, the protein need meet that First, biotin carboxylase catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the carboxyltransferase to acetyl-CoA to form malonyl-CoA.; Component of the acetyl coenzyme A carboxylase (ACC) complex4. The desired protein should be able to perform acetyl-CoA + N(6)-carboxybiotinyl-L-lysyl-[protein] = malonyl-   CoA + N(6)-biotinyl-L-lysyl-[protein]5. The protein should exhibit Basic and acidic residues compositional bias.6. We require a protein that can specifically bind to the metabolites produced in the Lipid metabolism pathway.7. A protein with carboxyl- or carbamoyltransferase activity, zinc ion binding, ATP binding is needed to enhance malonyl-CoA biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "MWRKCVKCLSPVYAEGVRNNYYICPKCGGYFRVHAYRRIEMICDKGSFEEWNKVMPVSNPLQFPGYEEKIAEAKLKSKLDEAVVTGKACIDGIPVVIAACDARFLMSSMGHNVGEKITYALERAADEKLPVIIYSCSGGARMQEGIVSLMQMAKTSAAVKKLHEAGQLYISFLTDPTTGGVTASFAMLGDIILAEPKALIGFAGPRVIAQTIGTKLPEGFQRAEFLLEHGFVDAIVERKDQKAVLGNILKTFKNRERCSKAAGTVKEVLSELDELRKKFKEKARKIYRDLSPWERVQVARHPKRPHTSDYIKYLIKDFEEVHGDTCYGDDKAVIAGFGYFRGKPVAVVGHEKGKDTKEKLERNFGMPHPEGYRKAIKVFKLAERYNIPVITFIDTPGAFPGIGAEERGQSRAIAESMLTMAFLKVPSVAVVIGEGGSGGALAFGVANRVCILENAYYSVISPEGCAAILWKDQSKVKEAAKALRLTAKDLKELGVVDCVIPEPYGAAHWSPRGTAMMVGMTLKKCLDKEPSPVADATLSSEEPNAAWKSVQLARNTHRPTAIYYIRNMIDGFIELHGDRAFGDDGAIVGGIGWLGGQVVTVIAEEKGENLKERIARNFGCPQPEGYRKSLRLMRQAEKFGRPIICLVDTQGAFCGAEAEERGQGNAIAENLQAMAGLRVPVVSVLLGEGGSGGALALAVSNRVAMQEHAVYSVLSPEGFASILWKDRSRAAEAAAVMRMNANQVYDMGIIDAVLAEGDAPAHENPEQAARELRHYLKRSLN",
    "response#2": "MWRKCVKCLSPVYAEGVRNNYYICPKCGGYFRVHAYRRIEMICDKGSFEEWNKVMPVSNPLQFPGYEEKIAEAKLKSKLDEAVVTGKACIDGIPVVIAACDARFLMSSMGHNVGEKITYALERAADEKLPVIIYSCSGGARMQEGIVSLMQMAKTSAAVKKLHEAGQLYISFLTDPTTGGVTASFAMLGDIILAEPKALIGFAGPRVIAQTIGTKLPEGFQRAEFLLEHGFVDAIVERKDQKAVLGNILKTFKNRERCSKAAGTVKPVTDPAVCGAKPAWDAVRCARDLTRPTTMDYIGLMCGDFVELHGDRLHADDPSIVGGLASIGGSTVMLMGHQKGHDTRELVRRNFGMPQPEGYRKALRLMRLAERLGIPVVTLVDTQGAYPGLGAEERGQAWAIAESITGMSELGVPVVSVIIGEGGSGGALALAAAERVLMLENACYSVISPESCSTILFGDPSHAEPVAEALRLTAPALLRLGVVDAVLPEPAGGAQAAPAEMAATLYRGIKLALDPVTDPAVCGAKPAWDAVRCARDLTRPTTMDYIGLMCGDFVELHGDRLHADDPSIVGGLASIGGSTVMLMGHQKGHDTRELVRRNFGMPQPEGYRKALRLMRLAERLGIPVVTLVDTQGAYPGLGAEERGQAWAIAESITGMSELGVPVVSVIIGEGGSGGALALAAAERVLMLENACYSVISPESCSTILFGDPSHAEPVAEALRLTAPALLRLGVVDAVLPEPAGGAQAAPAEMAATLYRGIKLALDGQLTPHQVLDARAALLRRMEDLPRREAWEAVRLARHPDRPTTAQYAEHLLEDFQELHGDRSTADCPALIGGPGRLDGRPVMLIGHDKGGPRLAERQRHHFGMATPAGYRKAARLMRMAAKLGLPVVALIDTPGANPGADAESGGQAVAIAENLRLMSRLPVPIVAAITGEGGSGGALALAVADRVLISVNGIYSVISPEGCAAILWKDAEAAPEAASALRLHARDLLRLGVVDGVVPEPEGGAHEDHTEAAALLGRALTATLG",
    "response#3": "MWRKCVKCLSPVYAEGVRNNYYICPKCGGYFRVHAYRRIEMICDKGSFEEWNKVMPVSNPLQFPGYEEKIAEAKLKSKLDEAVVTGKACIDGIPVVIAACDARFLMSSMGHNVGEKITYALERAADEKLPVIIYSCSGGARMQEGIVSLMQMAKTSAAVKKLHEAGQLYISFLTDPTTGGVTASFAMLGDIILAEPKALIGFAGPRVIAQTIGTKLPEGFQRAEFLLEHGFVDAIVERKDQKAVLGNILKTFKNRERCSKAAGTVKVLEKQACPAWESVQISRKKGRPVGIDYISHLFTDFMELHGDRYYKDDPAVVGGLAYFQGIPVTVIAQAKGKSTKENVERNFGMPSPDGYRKSLRLMKQAEKFHRPVICFVDTPGAFCGLEAEERGQGEAIAKNLFELSALKTPILSIIIGEGGSGGALAMAVADEVWMLENSIYSILSPEGFASILWKDSKRADEAAEVMKLTASDLKNMGIIEQIIPEPADYTEGSMDSVCETIKPGIAGFLEPVTDPAVCGAKPAWDAVRCARDLTRPTTMDYIGLMCGDFVELHGDRLHADDPSIVGGLASIGGSTVMLMGHQKGHDTRELVRRNFGMPQPEGYRKALRLMRLAERLGIPVVTLVDTQGAYPGLGAEERGQAWAIAESITGMSELGVPVVSVIIGEGGSGGALALAAAERVLMLENACYSVISPESCSTILFGDPSHAEPVAEALRLTAPALLRLGVVDAVLPEPAGGAQAAPAEMAATLYRGIKLALDWLVPRWM"
  },
  {
    "reference": "MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEIGVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTAIDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAKNVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTENIIRAVKGENIGTTVK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that can modulate the overall flux of metabolites through the Pyrimidine metabolism, CTP biosynthesis via de novo pathway pathway, enhancing or suppressing its activity as needed.2. We need a protein that can perform ATP + UMP = ADP + UDP with high efficiency.3. A protein with ATP binding functions is required to enable 'de novo' CTP biosynthetic process, phosphorylation.4. For general function, the protein need meet that Catalyzes the reversible phosphorylation of UMP to UDP.5. The designed protein should have a high specificity for ATP, UMP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MRVLLKISGERLAARGVSVHDAEVIGMLARGIKRVHEMGIQVCLVIGGGNIYRGSSGIPNIDRATGDYMGMLATVINALALQGAINNLGVVSRVQSAIPMRSICEPYVRQKAISHMEKNRIVIFAAGTGNPFFTTDTAAVLRSVEMGCDVMLKGTLVDGIYSDDPRTNINAERITKLSYTDVLSKKLRVLDSSAVSIARDNGMPVVIFRVLVKFSGEALAGENGFGIDTKILGFIASEIRSLVENKIEVGIVIGGGNIIRGVSAAQDGVIRRTSADYMGMLATVINAVAMQEALEHAGLDVRVQSAIELKEICESYIYRRAIRHLEKGRVVIFGAGTGNPFFTTDTAATLRAIEIGANMIIKATKVDGVYDKDPNKFSDAKKLDTLSYEEALDDRIKVMDDTAIALAKDNRLPIVVCDGRAGNEWLSSAPGTRIERVL",
    "response#2": "MAKRVLFKISGEALSDGDSSNRISEERLSRLIAELKVVRNADVEVALVIGGGNILRGLSQSQSLQINRVSADQMGMLATLINGMALADALKTEDVPNLLTSTLSCPQLAELYNPQKASDALSQGKVVICTMGAGAPYLTTDTGAALRACELKVDVLLKATMHVDGVYDQDPRECADAVRYDHISYRDFLSQGLGAIDPAAISLCMEAGIPIKMMS",
    "response#3": "MGYRVLVKFSGEALAGENGFGIDTKILGFIASEIRSLVENKIEVGIVIGGGNIIRGVSAAQDGVIRRTSADYMGMLATVINAVAMQEALEHAGLDVRVQSAIELKEICESYIYRRAIRHLEKGRVVIFGAGTGNPFFTTDTAATLRAIEIGANMIIKATKVDGVYDKDPNKFSDAKKLDTLSYEEALDDRIKVMDDTAIALAKDNRLPIVVCNLYDEHGLLKGILRGEAIGTVVQA"
  },
  {
    "reference": "MWTADEIALLCYEHYGIRLPKKGKPEPNHEWTLLAAVVKIQSPADQDCDTPDKPAQVTKEVVSMGTGTKCIGQSKMRKSGDILNDSHAEVIARRNFQRYLLHQLQLAATLKEDSIFVPGTQKGLWKLRRDLFFVFFSSHTPCGDASIIPMLEFEDQPCCPVIRDWASSSSVEASSNLEAPGNERKCEDLDSPVTKKMRLEPMTAAREVTNGATHHQSFGKQESGPISPGINSCNLTVEGLAAVTRIAPGSAKVIDVYRTGAKCVPGEAGDSRKPGAAFHQVGLLRVKPGRGDRTRSMSCSDKMARWNVLGCQGALLMHFLEEPIYLSAVVIGKCPYSQEAMQRALTGRRQNVSALPKGFGVQELKILQSDLLFEQSRCAVQAKRADSPGRLVPCGAAISWSAVPEQPLDVTANGFPQGTTKKTIGSLQARSQISKVELLRSFQKLLSRIARDKWPDSLRVQKLDTYQDYKEAASSYQEAWSTLRKQAFGSWIRNPPDYHQFK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must bind to 1D-myo-inositol hexakisphosphate in order to perform its enzymatic function.2. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding.3. The protein must have high specificity towards metal ion binding. The designed protein sequence is ",
    "response#1": "LEVITQLRFPVSDSERFGVPEQVVVMDSSVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNHGGVIFKVYVVGDHVKCVKRRSLPDISEEKIGTSKGSLPFSQISNLTAQEDKNIEYGEDRSLEKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKMPSYEPVLTEFFWDMVTKKNN",
    "response#2": "SLGTGNRCLGKSSLSNQGDVLNDSHAEIICKRSFQKFCYNEILNLLQSKYYNSILFNIEYHDSNNNNKDNDNNGSLPTISIKKGHSLHFYVNQTPCGDCSIFPFKKETQPENFIEKEKLEKDGKDKIENHEKKEQKDIIKQVDKDKDEENYEDEESKRKLKKVKDDNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNINNNNNQYDDIQRTGAKTVFGEPEDKKLIGVDYHQIGVLRVKPGRGDPTVSMSCSDKIARWNVLGIQGSLLSHFIKEQIFLSSITIGDLFNHSSIYRGLIGRLLPNPTTTTTETSSSSSSSSSSSNTIPNFKLNSDLEIFSTNIQFQFSKLLLESDQQNNNKSTSSGLAISFCYPNQHEVTIAINGKKMGTNQKNFNAISQRSSICKFNLFKLFHQLVLIIKNKNSNEENEKNNQIVLIDSLFNYYECKHLSKKYYQEYEKLKEFKFKNWLTNSSDLENC",
    "response#3": "SLGTGNRCLGKSSLSNQGDVLNDSHAEIICKRSFQKFCYNEILNLLQSKYYNSILFNIEYHDSNNNNKDNDNNGSLPTISIKKGHSLHFYVNQTPCGDCSIFPFKKETQPENFIEKEKLEKDGKDKIENHEKKEQKDIIKQVDKDKDEENYEDEESKRKLKKVKDDNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNINNNNNQYDDIQRTGAKTVFGEPEDKKLIGVDYHQIGVLRVKPGRGDPTVSMSCSDKIARWNVLGIQGSLLSHFIKEQIFLSSITIGDLFNHSSIYRGLIGRLLPNPTTTTTETSSSSSSSSSSSNTIPNFKLNSDLEIFSTNIQFQFSKLLLESDQQNNNKSTSSGLAISFCYPNQHEVTIAINGKKMGTNQKNFNAISQRSSICKFNLFKLFHQLVLIIKNKNSNEENEKNNQIVLIDSLFNYYECKHLSKKYYQEYEKLKEFKFKNWLTNSSDLENC"
  },
  {
    "reference": "MGWDRRHGGRHRLSSAGKDRAVTGRPILTAAEMRAAEVAVFATGVTVEQAMERAGAAVAEVAWRYAGRAPALVVCGPGNNGGDGYVIARRLKDRGVPVRVAASGEPRTDAARANRGRWDGPVETLADAKPARLLIDALFGSGLVRALDPAIAGPLERLAAAAQMRLAVDLPSGIGSDDGALLGPAIAYDVTVALAVLKPAHRLQPAAALMGRLIVADIGIAGTSRLSEIARPHLPAPGPDDHKYTRGHVVVVEGAMPGAAMLSAMAAQRGGAGYVTLTGHGTGGPAAIVRRGGDLADILVDRRVGAALIGPGLGREAAARALLDAALASKKPLILDADALMLLTERRPLQGMPILTPHEGEFAELFGELPGNRVERAGAAAANAQAVIVLKGPDTVVAAPDGRAAISVPAPGWLATAGTGDVLAGLIAANRARGLDAFEAACAGVWLHRRAAELAGPGLIADDLIGHLPQALAECL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein with ATP binding is needed to enhance phosphorylation, nicotinamide nucleotide metabolic process in a specific context.2. The protein should be designed to have high stability and activity towards (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "LILGGGAGHGGAARLAARAALRIGAGLVTLACPEPALAENAARLDAVMLRPLADAAVLEALLADARISALCLGPGLGLGPREAGLVRAALASRRPLVMDADALTLLARDPGLFALLHDRCVLTPHAGEFARLFPDLAERLAAPAPPGPAFSKLDACRAAAARAGCTVLMKGPDTVIAAPDGRALLNPALREREAPWLATAGAGDVLAGMIAGLLARGWTPLDAAGSAAFLHVEAARR",
    "response#2": "LTPMIAAGLLKKRDKYAHKGNFGHASLLAGSTGNMGAAVLAARACMRSGAGKLTCYVPAPGLGVLQTTVPEAMVRVSGSGNEIEGYFPLADHQGLGIGLAIGLGEKQRDLFRQVCNTHLRPMVIDADALTLLGKYPELWEFVPRDSILTPHPGEWERLFGKTANDFERMTKTSEAATQRQINIVLKGHYSFIALADGTAWFNSTGNPGMATAGSGDVLTGILTGLLAQGYTPGESTLLGVTLHGLAGDLALETGSEPSLIASDLVDHIGAAYVLV",
    "response#3": "GVVAGGEDYTGAPILACTAAVSAGVGMVRYVGTPTPEQLVRTLVPEVVLGPGRVQAWVVGPGLDVTSRKAGAKAQVDVAKEALSSDLPVLVDAGGLDLVHGRRSAPTLLTPHAGECARLLTRLARRKRAITPDEVKAAPLHHARRLAALTGASVLLKGSTTLVVSPDPDAPVHSQRDAPPWLATAGAGDVLAGLVGALLASGLEPTAAGALGALVHGVAADAAR"
  },
  {
    "reference": "MDKLKAYKALTKVIPQDLVLLDEPMKNHTSFKIGGPADIMVIPETIDQLKSAIKLSKENQMPYFIIGNGSNLIVRDKGMRCIVIKIAEQFSKVSFQGNTVIAEAGILLSKLSKKIMAESLKGFEFASGIPGTLGGAITMNAGAYGGEMKDVVKGAHLLNDNGEVRYFTLEELELGYRTSIIQKQGYIALDVELALEKGDYQEILEITRDLTERRTTKQPLHLPSAGSVFKRPEGYFAGKLIQDSGLKGQRVGGAQVSELHSGFIVNVGDATAKDVLDLIQLIKDRVYETFHVQLETEVRIVGEE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design must be able to perform UDP-N-acetylmuramate dehydrogenase activity in order to contribute to cell division, regulation of cell shape. The designed protein sequence is ",
    "response#1": "RGGKRLTGEVRISGAKNAALPLLASSILVDGAMTFTNVPDLVDVRSIKMLLRDLGAGCEEGEGTVLIDGSGIHKIEAAYELVRKMRASILVLGPLVARFGHARVSLPGGCAIGARPVDMHLKGLKALGATITIENGYIEAKADRLKGNEIYFDIPTVTGTENLMMAATLAKGTTVLRNCAREPEITALANALNLMGARVTGAGTAVIRINGVDGLSPATIHVIPDRIEAGTFMVAAAATGGDVLVTGCEPENMGGIINKLRQTGALVEIIDHGVKVSGGKSILSTDIKTLPYPGFPTDMQAQFMVLMAISQGNSVIHETIFENRFMHVNELQRMGADISISGGNYAMVRGVNALSGAEVMASDLRASASLVIAGLVARGVTTIHRVYHIDRGYEALEKLG",
    "response#2": "RGGKRLTGEVRISGAKNAALPLLASSILVDGAMTFTNVPDLVDVRSIKMLLRDLGAGCEEGEGTVLIDGSGIHKIEAAYELVRKMRASILVLGPLVARFGHARVSLPGGCAIGARPVDMHLKGLKALGATITIENGYIEAKADRLKGNEIYFDIPTVTGTENLMMAATLAKGTTVLRNCAREPEITALANALNLMGARVTGAGTAVIRINGVDGLSPATIHVIPDRIEAGTFMVAAAATGGDVLVTGCEPENMGGIINKLRQTGALVEIIDHGVKVSGGKSILSTDIKTLPYPGFPTDMQAQFMVLMAISQGNSVIHETIFENRFMHVNELQRMGADISISGGNYAMVRGVNALSGAEVMASDLRASASLVIAGLVARGVTTIHRVYHIDRGYEALEKLG",
    "response#3": "RGGKRLTGEVRISGAKNAALPLLASSILVDGAMTFTNVPDLVDVRSIKMLLRDLGAGCEEGEGTVLIDGSGIHKIEAAYELVRKMRASILVLGPLVARFGHARVSLPGGCAIGARPVDMHLKGLKALGATITIENGYIEAKADRLKGNEIYFDIPTVTGTENLMMAATLAKGTTVLRNCAREPEITALANALNLMGARVTGAGTAVIRINGVDGLSPATIHVIPDRIEAGTFMVAAAATGGDVLVTGCEPENMGGIINKLRQTGALVEIIDHGVKVSGGKSILSTDIKTLPYPGFPTDMQAQFMVLMAISQGNSVIHETIFENRFMHVNELQRMGADISISGGNYAMVRGVNALSGAEVMASDLRASASLVIAGLVARGVTTIHRVYHIDRGYEALEKLG"
  },
  {
    "reference": "MKLVLATSNKGKVREIQALCEDYEVIPYSELIQEFEIVEDGSSFKENALIKARAVYKALNNEDVIVLADDSGISVDVLDGKPGIYSARHAGSDANDKDNLYKLIEDIKAENVESSPAHYTAAIAIVTKNGEYSVHGWMHGTALAKAIGDGGFGYDPMFIPLGYDKTLGELNDEIKKKLSHRAKALSLAKKILQSI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+).2. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..3. The protein requires Mg(2+) to activate a critical catalytic site.4. The designed protein should have a high specificity for Mg(2+) binding, with minimal off-target effects.5. The designed protein should be optimized for XTP diphosphatase activity, dITP diphosphatase activity, ribonucleoside triphosphate phosphatase activity, ITP diphosphatase activity, metal ion binding activity in order to improve purine nucleoside triphosphate catabolic process, nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "MICAAGDYYCIDDYMKCFEEIALKHNMDIVYFPKQNEDVLLKDVLKILKNYSEKKIILSIGGDTLVNFAKAFDIDFIAIPTTCGTGSDVTPFVTIWDEEKKKSLAVKAIKPLYAVLVDKEILCDTPYDVAANTGFDALTHAIEAYVSKKSNILTDMYAIEAIRLIGDNLERVVKNGVDREARYNMALASTLAGMVFSHSRLGNVHAIANTIGGLYDIPHGVANALLLPYVMEFNYEACKDKFIEIAKALNLDVEGENLEDKVVEFIRKIGKEINLKLKLSDYGIDEEKFEEIVEKALKDKTIWNMPYEVSKEKVIKILKLV",
    "response#2": "MKTLVFATNNRHKLEEVQALLPADVELLSYADIGCVDEIPDTGDTLEGNARQKAHYLREHYQMDCFADDTGLEVDALNGEPGVYSARYAGEGRDAVANMDKLLRNLEGKTDRSAQFRTVISLVIGGKEQLFEGVCEGEITEAPAGAGGFGYDPVFVPDGYTQTFAELSSDVKKAISHRALATQKLLDYLRAH",
    "response#3": "MKYILATNNPHKVREIQAILPKGIDLLSLKDIGCSEDIPETADTLEGNARLKAEYVHQKYGYDCFADDTGLEVNALGGAPGVYSARYAGPEKDANQNIQKLLDELRGKTNRKARFRTVIALNLEGKQHLFEGVCEGIITEEPKGERGFGYDPIFMPDGYTQTFAEIGEEVKNKISHRAKAIDKLVEFLLNA"
  },
  {
    "reference": "MFHLLIYPLWVLVALFAVIIANLLYQQLPRRPDEPPLVFHWFPFFGNAVAYGLDPCGFFEKCREKHGDVFTFILFGRKIVACLGVDGNDFVLNSRLQDANAEEVYGPLTIPVFGSDVVYDCPNSKLMEQKKFVKFGLTQKALESHVQLIEREVLDYVETDPSFSGRTSTIDVPKAMAEITIFTASRSLQGEEVRRKLTAEFAALYHDLDLGFRPVNFLFPWLPLPHNRKRDAAHIKMREVYMDIINDRRKGGIRTEDGTDMIANLMGCTYKNGQPVPDKEIAHMMITLLMAGQHSSSSASSWIVLHLASSPDITEELYQEQLVNLSVNGALPPLQYSDLDKLPLLQNVVKETLRVHSSIHSILRKVKRPMQVPNSPYTITTDKVIMASPTVTAMSEEYFENAKTWNPHRWDNRAKEEVDTEDVIDYGYGAVSKGTKSPYLPFGAGRHRCIGEKFAYVNLGVIVATLVRNFRLSTIDGRPGVPETDYTSLFSRPAQPAFIRWERRKKI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: The C-22 sterol desaturases ERG5A and ERG5B further convert 5-dehydroepisterol into ergosta-5,7,22,24(28)-tetraen- 3beta-ol by forming the C-22(23) double bond in the sterol side chain; Eburicol is the substrate of the sterol 14-alpha demethylase encoded by CYP51A, CYP51B and CYP51C, to yield 4,4,24- trimethyl ergosta-8,14,24(28)-trienol; The third module or late pathway involves the ergosterol synthesis itself through consecutive reactions that mainly occur in the endoplasmic reticulum (ER) membrane; Sterol 14-alpha demethylase; part of the third module of ergosterol biosynthesis pathway that includes the late steps of the pathway.2. A protein that is capable of catalyzing reactions within the Steroid metabolism pathway with high efficiency and specificity.3. The protein must exhibit oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen. The designed protein sequence is ",
    "response#1": "QDKIEEIFERFKPHAVIHTASPSYMDTKKTLMSKNVDGTKALLEAARTCSDTKAFVFTSSDEAIMPTQEPTSEENAHRYDENNAPNAPNTYALSKALAKRLVIAANSEELYTSVIRIPGIYGKYDDNFIPQLVSSMREKEHKMQVGNNTKVFEFLYVNKAAEAHIMAMKALLNPSTRDQVGGEDFFISDGKPQGLFDFCRRIYAAAGL",
    "response#2": "GSGFLGGHIIRQLCERENLRIAAFDLFENEKLLHELHGQFTMYTGDLTKQGDIERVFEEFHPRVVIHTASPVHNLARDIYFEVNVDGTANIIKACQKFNVDALVYTSSAGVVFNGADLINVDESQPIPEVHMDAYNESKALAEKQVLEASSESLKTAALRVAGLFGPGDRQLVPGMLSVLKNGQTKFQLGDNLNLFDFTYIENAAYAHLLAMDNLLSSNPTANGQVFFITNGQVIYFWDFARAIWAHAGHVPPYIIKFPARYDMFTPPDLIIGGSGFLGLHLIEQFYRHCPNVAITVFDVRPLPEKLSKYFTFDPSKIQFFKGDLTSDKDVSDAINQSKCDVIVHSASPMHGLPQEIYEKVNVQGTKNLLSVAQKLHVKALVYTSSAGVIFNGQDVINADETWPYPEVHMDGYNETKAAAEEAVMKANDNDQLRTVCLRPAGIFGPGDRQLVPGLRASAKLGQSKYQLGDNNNLFDWTYVGNVADAHVLAAQKILDKSTRDDISGQTFFITNDSPTYFWTLARTVWKNDGYIDKYYIKLGSGFLGGHIIRQLCERENLRIAAFDLFENEKLLHELHGQFTMYTGDLTKQGDIERVFEEFHPRVVIHTASPVHNLARDIYFEVNVDGTANIIKACQKFNVDALVYTSSAGVVFNGADLINVDESQPIPEVHMDAYNESKALAEKQVLEASSESLKTAALRVAGLFGPGDRQLVPGMLSVLKNGQTKFQLGDNLNLFDFTYIENAAYAHLLAMDNLLSSNPTANGQVFFITNGQVIYFWDFARAIWAHAGHVPPYIIKFP",
    "response#3": "GSGFLGGHIIRQLCERENLRIAAFDLFENEKLLHELHGQFTMYTGDLTKQGDIERVFEEFHPRVVIHTASPVHNLARDIYFEVNVDGTANIIKACQKFNVDALVYTSSAGVVFNGADLINVDESQPIPEVHMDAYNESKALAEKQVLEASSESLKTAALRVAGLFGPGDRQLVPGMLSVLKNGQTKFQLGDNLNLFDFTYIENAAYAHLLAMDNLLSSNPTANGQVFFITNGQVIYFWDFARAIWAHAGHVPPYIIKFPGSGFLGGHIIRQLCERENLRIAAFDLFENEKLLHELHGQFTMYTGDLTKQGDIERVFEEFHPRVVIHTASPVHNLARDIYFEVNVDGTANIIKACQKFNVDALVYTSSAGVVFNGADLINVDESQPIPEVHMDAYNESKALAEKQVLEASSESLKTAALRVAGLFGPGDRQLVPGMLSVLKNGQTKFQLGDNLNLFDFTYIENAAYAHLLAMDNLLSSNPTANGQVFFITNGQVIYFWDFARAIWAHAGHVPPYIIKFPTSIMYCHYVAGLVGEGLTNFGSGFLGGHIIRQLCERENLRIAAFDLFENEKLLHELHGQFTMYTGDLTKQGDIERVFEEFHPRVVIHTASPVHNLARDIYFEVNVDGTANIIKACQKFNVDALVYTSSAGVVFNGADLINVDESQPIPEVHMDAYNESKALAEKQVLEASSESLKTAALRVAGLFGPGDRQLVPGMLSVLKNGQTKFQLGDNLNLFDFTYIENAAYAHLLAMDNLLSSNPTANGQVFFITNGQVIYFWDFARAIWAHAGHVPPYIIKFP"
  },
  {
    "reference": "MAHQSDLISEDILEYLKSQENKSLLRFITCGSVDDGKSTLIGRLLYDSKLIFEDQLSSLESDSKKVGTQGGDMDFALLVDGLAAEREQGITIDVAYRFFSTDKRKFIVADTPGHEQYTRNMATGASNADLAIILIDARKGILTQTRRHSYIVSLLGIKHVLLAVNKMDLMDYDQKVFEEIETDYRAFAKDIGLTDITCVPISALKGDNILERGDNLDWYDGPTLMEVLETVDVTTDEKSAPFRFPVQWVNRPDLDFRGFSGTIVSGTVRQGDEIVVLPSAKTSRIKCIVAMGGDLDEAIAGQAVTLVLEDEIDISRGDVICRKENRAELTDQFMAHVLWMDEEHLLPGRPYLVKIGSKMVPGMITDLKYKVNVNSLEHEAGKALELNEVGVCNLSLSDQIPYDHYADIRETGAFIIINRLNNNTVGVGMIDHGLRRAENIHWQALDVDKTSRAGQKSQKPAVLWFTGYSGSGKSTVANALEKKLFGRGNHTYVLDGDNVRHGLNKDLGFTDVDRVENIRRVAETSKLMVDAGLITLVSFISPFRSERRMARELMDDGEFIEIFVNTPLEVCEQRDPKGLYKKARAGEIKNFTGFDSPYEEPDDAELNIDTTDLQPDQAADRIIDYLEEKGYLAS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that APS synthesis involves the formation of a high-energy phosphoric- sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.; With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway2. A protein with sulfate adenylyltransferase (ATP) activity is needed to enhance hydrogen sulfide biosynthetic process, phosphorylation in a specific context.3. The protein should be designed to catalyze the following reaction adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate   + ADP + H(+).4. The presence of Tr-type G domains is critical for the interaction of the designed protein with its intended targets.5. A protein that is capable of catalyzing reactions within the Sulfur metabolism, sulfite from sulfate: step 2/3, hydrogen sulfide biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "TMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALENDSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADCPGHEQYTRNMVTGASTADLAVILIDARKGVLVQTRRHSYLCHLLGIRNIVLAVNKMDLIDYDQARYDAIVAEYETFAKGIGIESFTAMPISGFKGDNIRFLTCGSVDDGKSTLIGRLFYDAKLIYEDTLKSLEQDSATVGTTGGELDPALFTDGLKAEREQGITIDVAYRYFSTTKRKFIIADTPGHEQYTRNMATGASTCDLAIILVDARNGVVTQTKRHSFIVSLLGIKHVLVTINKMDLVDYDEEVFDRIRSDYRDFASRLEMPDLHFMPVSALKGDNIVDASSNTPWYQGPTLMSFLDNVYIGKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMRVGDALDARVVWMADTSLMPGRQYDLKMGPKTTSAIPELVHYRVDVNTLTHEHADQLGLNDIGLVRWRLNAPVPFDSYQRNPVTGAFIIIDRITNLTVGAGM",
    "response#2": "TMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALENDSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADCPGHEQYTRNMVTGASTADLAVILIDARKGVLVQTRRHSYLCHLLGIRNIVLAVNKMDLIDYDQARYDAIVAEYETFAKGIGIESFTAMPISGFKGDNIFRMPVQWVNRPNLDFRGFSGLVASGTVRAGDSIRVLPSGHTSTVKSVVAYGGDLDMAQAGQSVTICLEDEIDCSRGNVIAPDLDARVVWMADTSLMPGRQYDLKMGPKTTSAIPELVHYRVDVNTLTHEHADQLGLNDIGLVRWRLNAPVPFDSYQRNPVTGAFIIIDRITNLTVGAGMVSDQFAAHVVWLAEQEMIPERPYLCRIGTATVQARITRPKYKINVNTLEHTATTTLGVNEIGVCNVSFDRPVPFDAYAEGGPTGAFVLIDRITGNTVGAGMVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMVRG",
    "response#3": "SLMRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAILESDSKKVGTQGEEIDFALLVDGLAAEREQGITIDVAYRFFTTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILVDARKGVLVQTRRHSYLVHLLGIKHVVLAVNKMDLVDYDQATFDAIVDDYTAFAKEVGIEEFTAIPISGFKGDNIRFITCGSVDDGKSTLIGRLLYDSKALHTDQLDALDADSRRVGTQGEKPDFALLVDGLSAEREQGITIDVAYRFFATENRKFIVADCPGHEQYTRNMVTGASTADAAVILIDARKGVLRQTRRHSFLCHLLGIEDVILAVTKMDLVDWSQRAFDDIVGAYQEFASTLGLKRVTAIPVSGIEGDNIAQQSTSMDWFRGPTLIEALEGIEKSLLRFLTCGSVDDGKSTLIGRLLSDAKQIFEDQLSALERDSRKHGTTGEDIDFALLVDGLEAEREQGITIDVAYRFFATSKRKFIVADTPGHEQYTRNMATGASTADLAIVLVDARQGVLRQTRRHSIIASLLGIRHIVLAVNKIDLVGFDRAVFERIVGEYSRFAQDLGFASVTAIPMSARYGDNVSRRSDETGWYSGPTLIEHLETVDIDKSLLRFLTCGSVDDGKSTLIGRLLSDAKQIFEDQLSALERDSRKHGTTGEDIDFALLVDGLEAEREQGITIDVAYRFFATSKRKFIVADTPGHEQYTRNMATGASTADLAIVLVDARQGVLRQTRRHSIIASLLGIRHIVLAVNKIDLVGFDRAVFERIVGEYSRFAQDLGFASVTAIPMSARYGDNVSRRSDETGWYSGPTLIEHLETVDID"
  },
  {
    "reference": "MATAPYNYSYIFKYIIIGDMGVGKSCLLHQFTEKKFMADCPHTIGVEFGTRIIEVSGQKIKLQIWDTAGQERFRAVTRSYYRGAAGALMVYDITRRSTYNHLSSWLTDARNLTNPNTVIILIGNKADLEAQRDVTYEEAKQFAEENGLLFLEASAKTGENVEDAFLEAAKKIYQNIQDGSLDLNAAESGVQHKPSAPQGGRLTSEPQPQREGCGC",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be optimized for stability and solubility when expressed in cytosol, early endosome membrane, early endosome where it will be involved in phagolysosome assembly involved in apoptotic cell clearance. The designed protein sequence is ",
    "response#1": "QGCGELFEKAKQNNNNGEVSSGDNGLYFSNFSLFGTPVLKDISFSIERGQLLAVAGSTGAGKTSLLMMIMGELEPSEGKIKHSGRISFCSQFSWIMPGTLKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEVFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLRPDFSSKLMGFDSFDQFSAERRNSILTETLRRFL",
    "response#2": "TLQEAKKMCTRAQASLQSTTYQLVTQLPERLQFVTFMVDALKQQHELLTSIVESQSRIRKESVSQFNVQVERDLTPALAELDDILARLAKAKVPSYLINNVVHELEVHIDTLVEDRNLCDFISMDDIKLLTTNIEIYKANCTKLHGLLEKTMNESVIEPYQIIALKKYHNIMKQYKELMPVQLGMNLAPDVSPYKLNNLINTILKENSSLEHELVPMLEMMTNHYDQCVQGTLFFNTKNPKDPSGDVNFEVLRNDALELPDVLKELNTVYDIIVNNEVRTKKLLSSEFSRIDSLISMIKDLLEFYTDYKATHLSKYMILVLSCDEIFGKCSIAMNSNQTPLEAYIETITSLSYHYTQFLSIYKSEYLTELFYEQYTYPRKYLAKLTDFLNNDMFHLQQEEYERRKNWLARYGEFF",
    "response#3": "QGCGELFEKAKQNNNNGEVSSGDNGLYFSNFSLFGTPVLKDISFSIERGQLLAVAGSTGAGKTSLLMMIMGELEPSEGKIKHSGRISFCSQFSWIMPGTLKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEVFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLRPDFSSKLMGFDSFDQFSAERRNSILTETLRRFL"
  },
  {
    "reference": "MTARPDDTAPFSAVHRALVIVAAGSGTRLGHGIPKAAVTLGVGSILEHACQAVTPELGFELIVLVLPEQSSHHEVLSAPATALAHRTGSQVVITSGGAARTDSVRAGSQAVQKHAELQGWDGPVHVLVHDAARALTPPEVFSRVLQALADGAEAVTPAVPVTDTIKQVQSEGSAERVERTLPRSALRAVQTPQGFTLQFLERAFAHIEQLPTERASQLTDEAMIAEDLNTEVRVVPGHPHALKITTETDLITARALLAEQAREPLSSERSETGTSGAEAAATPPGPPRVGIGQDIHAFALADEPTELWLAGLYWPGQQGLAGHSDADPVAHAACAALFSAAGMGDLGTHFGADTLGTHRAEMAGARGVVLLSQAARIVRDAGFRIGSIAVQLVGNRPKFAPRREEAQRVLSEAAGAPVSVAATTSDGLGFTGRGEGIAATATAVLY",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must possess 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity, metal ion binding to support isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway under certain conditions.2. The designed protein should be able to compete with endogenous proteins or inhibitors for 4-CDP-2-C-methyl-D-erythritol 2-phosphate, a divalent metal cation binding.3. For general function, the protein need meet that Catalyzes the conversion of 4-diphosphocytidyl-2- C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).; Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds4. A protein should be able to manipulate the activity of enzymes in the Isoprenoid biosynthesis, isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6 pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "MKIRIGHGFDVHKFGGEPPLILGGVDVPYEVGLIAHSDGDVALHAISDAILGAMALGDIGKHFPDTDPEFKGADSRVLLRHCYQLATDMGFSLSNLDVTIIAQAPKMAPHIEAIRKVLAADLVADINDINVKATTTEKLGFTGRKEGIAVEAVVLMIEDEAEVLGAF",
    "response#2": "MKIRIGHGFDVHKFGGEPPLILGGVDVPYEVGLIAHSDGDVALHAISDAILGAMALGDIGKHFPDTDPEFKGADSRVLLRHCYQLATDMGFSLSNLDVTIIAQAPKMAPHIEAIRKVLAADLVADINDINVKATTTEKLGFTGRKEGIAVEAVVLMIEAEDSFINKEKDLIVMIHDGAGKSDADIILHAISDAILRVGHGFXVHKFGPGKGXILCGVHLPHDLSLXGHSDADVALHAITXAXLGALAXGDIGEHFPPTESEWXDVSSRXFLQKAIXLMEEKAGEIVXXDLTIVCELPKIGPFKPRMRQNLSEIFQTPMKNINIKATTTEGLGAMGRKEGISSHASVPLRIVKK",
    "response#3": "FVGSGFDVHEFEKGRPLVLCGEKIDYEFGLKAHSDGDVALHALTDAILGAAGLGDIGELFPDTDDKFKDISSIYLLQEAYKRVQSVGFVLTNADITIMAQKPKLSKLKSKMEANIAQALNLSQSRINVKATTTEGLGFVGRCEGIAVMASASGRIVARPPVTPRVGLGYDVHAFAENRKLILCGIDIPHERGLAGHSDADVGIHTLCDAIYGALAEGDIGQHFPPSDDKWRDMDSARFLVHAGDLIRSKGGMLVNADITIICERPKIGPHAQAMRERLAELLKVNVSRISVKATTSERLGFTGREEGIAATAAVSGEVIATPPVEPVGHGYDVHRLVEGRRLVLGGVEIPYALGLLGHSDADVLAHAVGDAVLGACGLGDLGRHFPDSDPRWKDASSLDLLRRIGELAAEAGYRVLRVDATLVAQRPKVAPHVPRMEANLSGGLGLPPGSVTVKATTTEGLGFEGRGEGISAHAVALGEIVANPPVEPATEPATEPATEPATEPATEPATEPATEPATEPATEPATEPSARPAYVAPPP"
  },
  {
    "reference": "MALTAEEKQEIIAKYATHEGDTGSPEVQVALLSKRIADLTEHLKEHKHDHHSRRGMQLMIGDRRRLLDYLKRVDINRYRSLIERLGLRR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that localizes to ribosome and can perform rRNA binding with high specificity and efficiency.2. For general function, the protein need meet that Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome. The designed protein sequence is ",
    "response#1": "MPNAHETE",
    "response#2": "ME",
    "response#3": "MK"
  },
  {
    "reference": "MSEGLWIATAVAAVLVVIAALILGSVRYRRRRISLIFSSLSSGPSASDRSGSYTASSGITFSQTALPVQPADRIDTGELPAVGDDATVPRDSPRHTISDVLLPESELITSPDEPEAAVPHIDAIVPPEGRLDRLRGRLARTQNAFGRSILGLIVGGDLDEDSWQELEDTLLVADLGPVATESVMSALRSRLLNSNVHTESDARTMLRDVLISELQPDMDRSIRALPHADHPAVLLIVGVNGTGKTTTVGKLARVLVADGRRVVLGAADTFRAAAADQLQTWASRVGAELVRGVEGADPASVAFDAVDKGIGAGADVVVIDTAGRLHTKVGLMDELDKIKRVVTRRAAVDEVLLVLDATIGQNGLTQVRVFAEVVDITGAVLTKLDGTAKGGIVFRIQRELGVPVKLVGLGEGPDDLAPFEPAVFVDALLE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECY",
    "response#2": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECR",
    "response#3": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECV"
  },
  {
    "reference": "MLLPGLLLLLLLLAGTRWLWGQWKLRKLHLPPLAPGFLHFLQPNLPIYLLGLTQKLGPIYRIRLGMQDVVVLNSNRTIEEALIQKWVDFAGRPHMLNGKMDLDLSLGDYSLMWKAHKKLSRSALMLGMRDSMEPLIEQLTQEFCERMRAQAGTPVAIHKEFSFLTCSIISCLTFGDKDSTLVQTLHDCVQDLLQAWNHWSIQILTIIPLLRFLPNPGLQKLKQIQESRDHIVKQQLKQHKDSLVAGQWKDMIDYMLQGVEKQRDGKDEERLHEGHVHMSVVDLFIGGTETTATTLSWAVAFLLHHPEIQKRLQEELDLKLGPGSQLLYRNRMQLPLLMATIAEVLRLRPVVPLALPHRATRASSISGYDIPKDMVIIPNIQGANLDEMVWELPSKFWPDRFLEPGKNPRTPSFGCGARVCLGEPLARLELFVVLARLLQAFTLLPPPDGTLPSLQPQPYAGINLPIPPFQVRLQPRNLAPQDQGERP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11- deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids2. The designed protein localizes to the endoplasmic reticulum membrane.3. The designed protein should have a binding site that is specific to the target 17alpha-hydroxyprogesterone ligand. The designed protein sequence is ",
    "response#1": "LVTGGCGFLGEHIVRMLLEREPRLRELRVFDLHLSSWLEELKAGPVQVTAIQGDVTQAHEVAAAMSGSHVVIHTAGLVDVFGKASPKTIHKVNVQGTQNVIDACVQTGTQYLVYTSSMEVVGPNIKGHPFYRGNEDTPYEAVHSHPYPCSKALAEQLVLEANGRKVNGGLPLVTCALRPTGIYGEGHQVMRDFYYQGLRFGGRLFRAVPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCGLRT",
    "response#2": "LVTGAGGFLGQRIVQMLVQEKELQEVRVLYRTFSPKHKEELSKLQTKAKVTVLRGDIVDAQFLRRACQGMSVIIHTAAALDIAGFLPRQTILDVNVKGTQLLLDACVEASVPAFIYSSSTGVAGPNSYKETILNDREEEHRESTWSNPYPYSKRMAEKAVLAANGSILKNGGTFHTCALRLPFIYGEESQIISTMVNRALKNNSIIKRHATFSIANPVYVGNAAWAHILAARGLRDPEKSQSIQGQFYYISDDTPHQSYDDLNYTLSKEWGFCLDSSWSLPQFVAGFR",
    "response#3": "LVTGGCGFLGEHIVRMLLEREPRLRELRVFDLHLSSWLEELKAGPVQVTAIQGDVTQAHEVAAAMSGSHVVIHTAGLVDVFGKASPKTIHKVNVQGTQNVIDACVQTGTQYLVYTSSMEVVGPNIKGHPFYRGNEDTPYEAVHSHPYPCSKALAEQLVLEANGRKVNGGLPLVTCALRPTGIYGEGHQVMRDFYYQGLRFGGRLFRAVPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCGLRV"
  },
  {
    "reference": "MVKAKVWILRKHFDGFPKESDFQMQGQVMLEAIFLSLDPYMSAALCSDTVFGCRVIQSKNRAYPVGCYVLAPCGWRTHTVTNGKAIFGQNMARVLPDWPKDIPLSMAIGALGMPGLTALYGLEEVLQVKPGEIVLVSAAAGAVGTMVGQICKIKGCKVVGSAGSEEKMAYLKELGFDHVFNYRTVSSLDEALKEASPEGYDCYFENVGGPFFTAALNQMRIGGRIAVCGAISLYNATTPQMCPYPHMTLLSRKIRIEGFQVDQWPDKDEASTRRLVKWMKEGKIKAKEKITEGFENMPAAFIRMLKGTDLGKAVVKV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein have Enoyl reductase (ER) domains with specific sequence motifs that are crucial for its activity.2. The protein should have a low Km value for leukotriene B4 + NADP(+) = 12-oxo-leukotriene B4 + H(+) +   NADPH.3. The protein can be targeted to cytoplasm and perform 2-alkenal reductase [NAD(P)+] activity, 13-prostaglandin reductase activity, 15-oxoprostaglandin 13-oxidase activity. The designed protein sequence is ",
    "response#1": "PTLENFELVEETVPELRDGEFMIRAMYLSVDPYMRIYTLSSPTGNTMIGGQVGEVIESRNPAFPVGAYAFAQVGWRTVSVCDPAAFETRKPYVLPELGDLSPSLGMGALGTVGNTAYFGFLEICKPQPGETVVVSGAAGAVGSLVGQIAKLKGCRVIGIAGTREKCAWLAELGFDGAINYREECVEEALRRHAPDGIDCYFDNVGGATSETVRGQMRPRGRISVCGTISMYNGAPVRVTDPQRDFVTKQLTQEGFSVHRWTDRWFEGIEQNLRWIREGKLRVRETVTVGFEHMPRAFIEMMAGGNVGKAVL",
    "response#2": "GVPKTTDFDLKIEDVKALEENEILCEGKYWSVDPYMRVYMDSHPVGVTMIGGQVAKVIETKHPKYPVNSWIFAYFGWRTHTVVNPDKVPEDAQFRSLPELGTLSPSLALGNLGMPGNTAYFGLMELCQPKSGETVVVSGAAGAVGSAVGQIAKIKGCRVVGIAGGTQKCSWLRKEMGFDATIDYKTENVRKALKECAPKGVDCYFDNVGGEISSAVISNMNLFGRIAVCGAISSYNDDVKSWPKVPHVQPEFVFKQLKMEGFIVTRWLHRWMEGILQIKEWMKEGKIKNPETFTEGFENLPRAFIEMLEGKNVGKAIIKF",
    "response#3": "GEPKLSDFEMKEEELEALQDGEFSVVAIFIGMNAGLRYYLDFTPIGSTVHAAQVAKVVDTRNKSFPIGATIYGSFGWRSHTVVKPENYAKHNLYTLPDFGKLSPSLGVGYLGMPGNTAYFGLLDICKPKEGETVVVTVAAGCVGSHVGQIAKIKGCRVIGFAGSDDKCKWIENELGFDKAINYKTGDMRKALKEAAPKGVDCYFDNVGGELSSIIISQMNNFGRISVCGAVAEYNSETEVKITSPWKHMILKQLKMQGFVVYSYGDRWMEGINQNLKWIQEGKLKYHETIFEGFDNIPQAFIDVLRGRNIGRGVL"
  },
  {
    "reference": "MRPKIPHHLSLHRVKSSTSSPSARDVAEPTTPSGNSRHDIPIRTPALEKDDMLRKKGKDMATSIPLSEQRGDHKNMALVLRVQVIRGRNLAPKDKSGTSDPYLVLTMGDKREATSIVSKTLNPEWNQTFDFPVLSAESALIEAVCWDKDRFKKDYMGEFDVMIEDHFAAGSTSPEPRWHKLEGRRSGKKQKKKKDDDVSGEVLLRFRLFNPLDTAATPQQILQRFYGVVAAAGADEDGDESDDDELLSRLNSHGLDDVSEEEDKERSDETADDEGMRTPGGTMILDSGEKQKRQRRQKLKKLKRKSKLKAYEFSGMTDVAGVLFLEIIRITDLPPERNMTRTSFDMDPFVVTSLGKKTYRTKVVNHNLNPVYDEKLVFQVQKHELNYSLSFAVVDRDKFSGNDFVGTALFPVEKVRSLAPEADPETGLYKLPDPDAITDSDTRRRKWRVPMSRSGSQNNLTKIQSRNNSQTNLNKLALTTSNTSVTNMAIAADVAPNKPGLVRQDSNSSFTERQHRRPAPTSHPSTSGGSAHGNGLHASVGSAHDHMADGSNDETGLYYYELPLELKNKSRWEEKYHPTLYIRVKYLPYDALRQQFWRVMLRQYDADESGKLDKVELVTMLDTLGSTLHNATINGFFKRFAVENGGEEVLTMDQAIMCLEQQLMTSQETHKHLHTPHWLHKRGDSESGAHPKMSTAGTGMSLNSSPEESPGGGTPYLGGADQTTPGNFSTKTNQIPTLEILDLSDAGERGERLPDDDLAGSASGIPGN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should contain one or more EF-hand domains that are essential for its function.2. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.3. The protein design should take into account the physiological conditions of the phosphatidylethanolamine biosynthesis pathway.4. For general function, the protein need meet that Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.5. The Charge relay system; for autoendoproteolytic cleavage activity active site of the designed protein must be optimized for substrate binding and specificity.6. The presence of pyruvate is crucial for the protein to function optimally under specific conditions.7. The designed protein should be expressed specifically in the endosome membrane, Golgi stack The designed protein sequence is ",
    "response#1": "MLRVQVVGCKDLLAKDRNGFSDPFLHVTLLPTRHVTPVAKRTLNPSYPEKESTFDFPIYLSLADRLGVLELVVWDKDLLRKDYLGEGGILVSDWFKDGLTLEL",
    "response#2": "VFMEIVKVTDLPPERNVTRTSFDMDPFVVTSLGRKTLRTPVIRHNLNPVYNEKMVFQVMKHEQSYTISFTVMDRDKFSGNDFVASAGFPLQTLIQL",
    "response#3": "MLEIQGAEDLPRLRNMTRTGWDMDPFVVISFGKKVFRTRVIRHSRNPSWDEKLLFHVRRYEASFNVQLAILDWDKLSANDHIGDVSFNVKELLECAPQPGG"
  },
  {
    "reference": "MIGIKILVNCALPYANGPLHVGHIAGAYLGADVFVRFNRLIGNEVLYVSGSDEYGTPITVRAEKEGKSPQEIADRYYAEHIETFKKLGINFDIFMRTTYPEHSSVAQEFFLKLLNEGVIEKGTMIAPYCRKIGRFMPDRYIEGECPYCGYKKARGDQCDNCGRTLDPQELINPVCILSGETPEFRETEHFFLRLDLFEERLEEWLKTKTFWKPNVLAYTRNFIQGGLKKRAITRDIDWGVKVPLEGYEHKRIYVWFEALIGYITGAKMFSEVIGKPNYWKEFYFDKDVKNYYFIGKDNIPFHSIIWPAMLLGYGELNLPYDIPANEYLTFKGEQFSKSRGIGYTVDELLRVIPPDYLRYYVASILPETGDSDFSLEELVKTVNSDLIDKYGNFVHRTLSFINKYDLKITKPNSLNDEAFEYAQNAFKEYCDELSQVHIKRSLAIWLNLAIYANSYFNKSEPWNLIKTDRDQCNYKLYVSLKLAQYLTAMIYPFTPTSAKAIWEQLGVNMDIDSSFSILNSINDFSVKPSRIPFEKLDIDKLKL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Incorporating a 'KMSKS' region, 'HIGH' region motif is critical for the successful design of the protein.2. The protein have enhanced ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-   methionyl-tRNA(Met).3. The protein must exhibit the following characteristics: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation..4. The Zn(2+), ATP binding site should be stable and able to withstand changes in environmental conditions.5. The protein should have methionine-tRNA ligase activity, ATP binding, metal ion binding and be active in cytoplasm. The designed protein sequence is ",
    "response#1": "MLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSHEKDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEVCGATYSPTDLINPVSVISGASPVEMESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGFPIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKASKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYTHGFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRVNSDLVGKVVNLASRVGKFASRTGLAPSYPEDGGLFQAAAAKGDEIASAYEDGEFSKAMRLIMELADAANPFVEHAKPWEMNKAPERQDELRDVCTVALNLFRQLAVYLAPVLPELAKKCGDLLDVTLQHNNEELAASWGNLVNRVANLINKNFGEIPAFDEASATDEDRALLAETKAAFETVGGLIENHRQKNALSEAMRVVGDINKYISATEPWKIKDNPARLGTVLHTAAQAVSDANHLLAPFLPHSAQKVWEAL",
    "response#2": "MLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSHEKDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEVCGATYSPTDLINPVSVISGASPVEMESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGFPIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKASKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYTHGFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRLVSVVELADTLGNFIKEDFMARVNADLVGKYINIASRAAGFISKRFDGRLGEPSADGDALLEVLRGASPAIQELYAEREYGKALREVMQLADRVNGYVDQNKPWELAKQPGMEARLLDVCTVCIEAFRLLTIYLKPVLPALGTQVEDFVNTDLVNKLVNLASRNAGFIQKRFDGKLADKLEDESLFAEFIAQSEQIAAYYENREFGKAIREIMALTDKANKYVDDKAPWVIAKEEGREAELQAVCSMGIQLFRVLMGYLKPVLPKLAERSEAFL",
    "response#3": "LDDFVARVNSDLVGKYINIASRCAGFISKRFGGKLVSGEDYRLLQQMVDEHFAGWQPGVIEAAYEARDFSAAVRHIMRRADEVNELIHELAPWEIARDETRERELHRACSLGIQMFYLLSCYLKPILPRTAAQIEDFL"
  },
  {
    "reference": "MSRVEAAAAEVKVRQHNALYLLSALCMVFMTLVVAIQPLFLRNVLNISFETAGAVNANVQVVTEVLDLFIFAYLGYLSDRIGRVRIIVAGFLVAAIGAVIAPLSPWIGGASIGALVVYYVSRVIMSAGSGAVWPQLSALAGDFSDDDTRARLMSNTAFMMAFGVTLVYAVLMQIPAHAGIAVTMLLTAAVSLAGAWLAGKFLVDVAPRTQETSVPWRAVWSLVKAEPRLRLAFASSLFARSDMVFVGLFLMLWFIYFADLIKVGQAEAAARAGILIGLMGAVVMLSIPVWRSFIEHFGRIQAVLLGMVLSALGFIMLGFIINPFDWFIVLPILLIASGQAGCFVAPQILTVDYAPRDLLGSVLGAFNVIGCIGIIFFVQVGGFLFDYVGPPAPFVFTGVGNLIISAYALRLLKREARDGGGDDAPGDDGVA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Partially functionally redundant with MamZ.2. The protein can be expressed in magnetosome membrane and perform transmembrane transporter activity without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "GVGAAGPAIFAGTRAPHTDGRQNMDCTTCHDLIPAGNGRPAPMMPIAAPIPPPPIPMGAVSPHTDGRQNMNCANCHQMLGGAAPIAAPGLGGGAYRFAQPPGSLAINIQGPRGGQSTAAGTGRVTLLGAALTPMSQRLGAQTGVPVGRGVFISGVTPNTPAATAGLRPGDVLLKVDGRR",
    "response#2": "GVGAAGPAIFAGTRAPHTDGRQNMDCTTCHDLIPAGNGRPAPMMPIAAPIPPPPIPMGAVSPHTDGRQNMNCANCHQMLGGAAPIAAPGLGGGAYRFAQPPGSLAINIQGPRGGQSTAAGTGRVTLLGAALTPMSQRLGAQTGVPVGRGVFISGVTPNTPAATAGLRPGDVLLKVDGRL",
    "response#3": "GVGAAGPAIFAGTRAPHTDGRQNMDCTTCHDLIPAGNGRPAPMMPIAAPIPPPPIPMGAVSPHTDGRQNMNCANCHQMLGGAAPIAAPGLGGGAYRFAQPPGSLAINIQGPRGGQSTAAGTGRVTLLGAALTPMSQRLGAQTGVPVGRGVFISGVTPNTPAATAGLRPGDVLLKVDGRR"
  },
  {
    "reference": "MSQRKPWEIIIRPIITEKSNRLMEDYNKYTFEVALDASKPEIKYAVEKLFNVKVKKVNTMIVKPKKKRVWNKFRQYGTTKKWKKAIVTLEKGHKIDILEFAQK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome; One of the early assembly proteins it binds 23S rRNA2. The protein must exhibit rRNA binding. The designed protein sequence is ",
    "response#1": "MI",
    "response#2": "MNP",
    "response#3": "MSPIGAKVTLRGERMYDFLDKLVNVSLPRVRDFHGVSAKSFDGRGNYTLGVREQLIFPEIDYDKVDRVRGLDVVIVTTSNTDEEARELLTQFGMPF"
  },
  {
    "reference": "MNALFAWTDGACSGNPGPGGWGVLMRAMDGDRMLKERELSGGEAETTNNRMELMAAISALEALTRPSEITVTTDSAYVKNGVTQWIHGWKKNGWRTADRKPVKNADLWQRLDAAQARHQVRWEWIKGHAGHPENERADELARAGMAPFKPARVSG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.2. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.3. For general function, the protein need meet that Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.4. The ideal protein would be one that plays a critical role in RNA catabolic process through magnesium ion binding within cytoplasm. The designed protein sequence is ",
    "response#1": "MNAVEIYTDGACKGNPGPGGWGAFLKSADSQKELFGGELGTTNNRMEMTAVIEALAALKRPCQVTLHVDSQYVLKGMTEWLAGWKARGWKTAAKQPVKNVDLWQRLDELVSTSGHRIDWRWVRGHNGDPGNEHADMLANRGVLV",
    "response#2": "MKEVTIYTDGACSGNPGPGGWGAVLIYGDKRKELSGAEPSTTNQRMEITAAIAALRVLKEPCRVHLYSDSAYLVNAFRQGWLARWERNGWLTVKKQPVENQDLWRELLQVASRHQVEWLKVKGHSDNPENNRCDELARAAIV",
    "response#3": "MNAVEIYTDGACKGNPGPGGWGAFLKSADSQKELFGGELGTTNNRMEMTAVIEALAALKRPCQVTLHVDSQYVLKGMTEWLAGWKARGWKTAAKQPVKNVDLWQRLDELVSTSGHRIDWRWVRGHNGDPGNEHADMLANRGVI"
  },
  {
    "reference": "MSHQSDLISQDILAYLSQHERKELLRFLTCGNVDDGKSTLIGRLLHDSKMIYEDHMEAITRDSKKSGTTGEEVDLALLVDGLQAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRNMATGASTCDLAIILIDARYGVQTQTKRHSFIASLLGIKHIVVAVNKMDLKDFDQGVFEGICNDYREFAAGLELLDGNSVHFVPMSALKGDNVVNRSERSPWYTGQTLMEILETVEIAADRNLTDLRFPVQLVTRPNLNFRGFAGTIASGVVHKGDELVVLPSGKRSQVKSIVTFDGELEVAGPGQAVTLTLEDEIDISRGDMLCHADNQPLIGDQFDATLVWMAEQPMQPGRKYDIKRATSNSPGSFTRIQHRIDVNTLEEQGAAQLGLNEIGRVELSLERPIAYDDYQTNHTSGAFIVIDRMTNGTVGAGMIVAKGAKGAKGEHVGGSQHGRLAHVTAQERADRFGQDPVTVLFTGLSGSGKSSIAYALERKLFDAGRACYVLDGKEMRQDLSKGLSMDAAGRAENLHKGVRIARQMNDAGLIAIGAFVAPTEESRATARYLLGERCLLVYLDTPMEVCQTRDPSGIYAANVEGTIPGVSFPYETPDDADLVLNTAELDLNGCVEKVVGLLRERKLI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design must be able to perform sulfate adenylyltransferase (ATP) activity in order to contribute to sulfate assimilation.2. The protein design should take into account the physiological conditions of the Sulfur metabolism pathway.3. The protein should be designed to catalyze the following reaction adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate   + ADP + H(+). The designed protein sequence is ",
    "response#1": "ELLRFLTCGSVDDGKSTLIGRLFYDAKLIYEDTLKSLEQDSATVGTTGGELDPALFTDGLKAEREQGITIDVAYRYFSTTKRKFIIADTPGHEQYTRNMATGASTCDLAIILVDARNGVVTQTKRHSFIVSLLGIKHVLVTINKMDLVDYDEEVFDRIRSDYRDFASRLEMPDLHFMPVSALKGDNIVDASSNTPWYQGPTLMSFFRMPVQWVNRPNLDFRGFSGLVASGTVRAGDSIRVLPSGHTSTVKSVVAYGGDLDMAQAGQSVTICLEDEIDCSRGNVIRDGFMTEKDYNGVVKDNRLADGALFSMPITLDVDQATIDEVKIAPGARITLRDFRDDRNLAILTVEDAKEVFGGDEEHPAIQYLYNTAKEFYVGGKLEAINEVSDQFEATIVWMDEEALLPGRGYWMKLASQTVSATVAEPKYEININNVGGPGDHLAAKTLELNSIGVAEVTTDRPVVFEPYADNRALGGFILIDKITNRTVGAGMLSFALREANNVRWQHLDVTREAHARIKGQRPAVVWFTGLSGSGKSTIANLVEQKLHALGKHTYILDGDNIRHGLNKDLGFTEADRVENIRRVGEVAKLMTDAGLIVLTAFISPFRAERQMVRDMMAEGEFIEIFVDTPLEVAEQRDVKGLYKKARSGQLPNFTGIDSPYEEPERAEIRIDTTTTSAEEAAELIIARLIEKGIL",
    "response#2": "PPHGGILKDLIARDAPRRQQLIDEAETLPAIVLNERQLCDLELILNGGFSPLEGFMNEKDYNGVVDNNRLVDGNLFSMPICLDVSQETIDEVKAKAGARITLRDFRDDRNLAIITIDDIYRPDKKKEAVEVFGADDEAHPAVKYLYRTAGDFYIGGKIDAIL",
    "response#3": "ELLRFLTCGSVDDGKSTLIGRLFYDAKLIYEDTLKSLEQDSATVGTTGGELDPALFTDGLKAEREQGITIDVAYRYFSTTKRKFIIADTPGHEQYTRNMATGASTCDLAIILVDARNGVVTQTKRHSFIVSLLGIKHVLVTINKMDLVDYDEEVFDRIRSDYRDFASRLEMPDLHFMPVSALKGDNIVDASSNTPWYQGPTLMSFL"
  },
  {
    "reference": "MALSTIEEAIQAIKNAEMVIVVDDEDRENEGDLIMAAEFATQEKIAFFTQHTSGLICVPIIGERLDELGIPLMVQNNTESNRTAFTVSIDALKGTSTGISAKDRATTILALIDEDTKPDELSRPGHIFPLRYREGGVLKRGGHTEAAVDLAIAAGLYPAGVICEIVSKDKGHMARFDELEALAAEHNLKMISIADLVRYRHQKEKLVRRVEDAQAKLPTVFGEFECVVYESLLDGEQHFAIYKGDLDDEDGVLVRVHSECLTGDVFGSLRCDCGPQLNAALEMIAEQGSGVVVYLRGHEGRGIGLAHKLRAYSLQEKGRDTVDANLELGLPIDSREYGIGAQILVDLGVSKMRLITNNPSKYGGLSGFGLDIVGRVGLNIEPNGYNIDYLKTKRDRLGHLIEGLDDSVFENK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must be able to 3,4-dihydroxy-2-butanone-4-phosphate synthase activity. The designed protein sequence is ",
    "response#1": "LLPSFWTEQLTLTKEPSEKALLQLSRFVYDLRDDDALFRATLLEDPSKKAPVNSSNNNGFDLMRKNHKHRREFSALTLATTGQSEVDWLLNLL",
    "response#2": "LLPSFWTEQLTLTKEPSEKALLQLSRFVYDLRDDDALFRATLLEDPSKKAPVNSSNNNGFDLMRKNHKHRREFSALTLATTGQSEVDWLLNLL",
    "response#3": "LLPSFWTEQLTLTKEPSEKALLQLSRFVYDLRDDDALFRATLLEDPSKKAPVNSSNNNGFDLMRKNHKHRREFSALTLATTGQSEVDWLLNLL"
  },
  {
    "reference": "MTKFIFVTGGVVSSIGKGIVAASLGRLLKSRGYSVSILKLDPYINVDPGTMSPYQHGEVFVTADGAETDLDLGHYERFTDTPMSRLNSVTTGSIYQAVINKERRGDYNGGTVQVIPHITAEIRDRIHRVAQDTHPDVVITEIGGTVGDIESLPFLEAIRQFRKDVGRRDLAYIHVTLVPWIPSAGEMKTKPTQHSVKELRSIGIQPDILVCRCDRPLQAGMKEKMSEFCNVSPEAVITSQDASSIYEVPLMLEREGLAEQVLDILQLEQRQPDLTQWQRWVHQLQNPSRRVEVAIVGKYVRLNDAYLSVSESLRHAAASADADLQLRWVDAEDLENGDPATYLDGVDGIVVPGGFGARGVDGKVAAIQFARDHQIPFLGLCLGMQAAVIDWARNVAGLDGANSAEFDPETPHPVIALLPEQQDVVDLGGTMRLGLCPCKIQSGSLAQRLYGEDIIYERHRHRYEFNNAYRSLFLESGYCVSGTSPDSRLVEIVERPDHPFFIACQFHPEFVSRPNHPHPLFQGLIKAALGSDLTLVDPAPLPAETV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that can modulate the overall flux of metabolites through the Pyrimidine metabolism, CTP from UDP: step 2/2, CTP biosynthesis via de novo pathway pathway, enhancing or suppressing its activity as needed.2. The designed protein must have a active site, Nucleophile; for glutamine hydrolysis active site that is highly conserved among related enzymes.3. A protein with metal ion binding, glutaminase activity is needed to enhance 'de novo' CTP biosynthetic process in a specific context.4. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen5. The protein contains novel Glutamine amidotransferase type-1 domains that confer a unique function or activity.6. The L-glutamine, UTP binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MEARGLRVTNIKLDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTLARMTRQNNFTTGRIYEAVISKERRGEYLGATVQVIPHVTDEIKLRIQSAAEGADVAIVEIGGTVGDIESLPFIEAIRQLKIESGSQNALSVHVTLVPFIATAGEMKTKPTQHAVKEMRAIGIQPDVLLCRCDRALPRDMKEKIALFSNVPVDRVVAAMDVACIYELPLALHAEGIDEKLTSTLGISPAPRPDLAHWNELVRRVKSPRHEVTIAIVGKYVKLHDAYLSVAEALRHAGYENESRINIKWIESENVTDETAAAFFADCNGILVPGGFGQRGIEGKITAAKYARENNIPYFGICLGMQIAVVEYARNVLGLELANSSEFDTTHDCVIDLMPDQQGNLPKGGTMRLGAYPCSIKSGTLLEKIYGVQEISERHRHRYEFNNDYRDIFVKNGMVFCGTSPDNRLIEAVELPQHCFFVAVQFHPEFKSRPNRAHPIFREFIAASLKHS",
    "response#2": "MEARGLRVTNIKLDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTLARMTRQNNFTTGRIYEAVISKERRGEYLGATVQVIPHVTDEIKLRIQSAAEGADVAIVEIGGTVGDIESLPFIEAIRQLKIESGSQNALSVHVTLVPFIATAGEMKTKPTQHAVKEMRAIGIQPDVLLCRCDRALPRDMKEKIALFSNVPVDRVVAAMDVACIYELPLALHAEGIDEKLTDALGIKTREPELSEWRTLVDRIKNPSGEVTIAIVGKYVKLHDAYLSVAEALRHAGYENESRINIKWIESENVTDETAAAFFADCNGILVPGGFGQRGIEGKITAAKYARENNIPYFGICLGMQIAVVEYARNVLGLELANSSEFDTTHDCVIDLMPDQQGNLPKGGTMRLGAYPCSIKSGTLLEKIYGVQEISERHRHRYEFNNDYRDIFVKNGMVFCGTSPDNRLIEAVELPQHCFFVAVQFHPEFKSRPNRAHPIFREFIAAAAKAQKR",
    "response#3": "MEARGLRVTNIKLDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTLARMTRQNNFTTGRIYEAVISKERRGEYLGATVQVIPHVTDEIKLRIQSAAEGADVAIVEIGGTVGDIESLPFIEAIRQLKIESGSQNALSVHVTLVPFIATAGEMKTKPTQHAVKEMRAIGIQPDVLLCRCDRALPRDMKEKIALFSNVPVDRVVAAMDVACIYELPLALHAEGIDEKLTQVLGMEPPPREDMRRWESIVRRVKSPRGEVVIGLIGKYVELQDAYKSILEALIHAGAMNECKVEVRNIHSEYLTKENAKEKLQNLDAVLVAPGFGSRGIEGKIEAIRYARVNKVPFFGICLGMQMACVEFARNVLGMPKAHSTEMDPDTKDGVISMMESQKTITMMGGTMRLGSYACELRPDSTTAEIYGTANITERHRHRYEFNNEYLAAFEEAGFIPVGKNPLTDLVEVVEMKDHPFYIGVQFHPEYKSTVENPHPLFVGFVEAAKEHQRLV"
  },
  {
    "reference": "MADPIVHPGVHRPEFSAPLHARAEIIATLRAALGKPNTTLVGAAIGTGMAAQAASRGGADFILALNAGRLRSMGAPSIFSLLALRKSNDFVLDFAQSEILPFVKVPVFFGASAFDPRCSIEAELERIADAGFGAIVNFPTSIFLDGRFRADIEGAGLGFQRELEMLRAAQKRNMATLAYVRTVAEAQQAATAGVDIINLNLGWNVGGTVGSRTELSLRQAAEYAKIIFRQIRAISEDTLCVLEGGPIVSPDQMYEVSALSKADGYIGGSTIDRVPLEASMEQITSAFKSVGTLQKRIDELERQLEHVQREYSIVGRSPSIQQIKQRIEKLAASSLPVMITGQAGTGKKLLARGIHEAARRSGSKLISSEDASGESLFGFAPSEGGRKVLGLLQYHPKATLLIESVECLCVDAQERLIEVIETGAYRRLGDNERGRFEGRLILASTRPLPELGSSGQLIPALESRLAPGHVFLPPLCDRLEDLPLLAEHFLQALRKDRRSRKLSVDHSAYRVLMTYGWPENIRELRSVLETAAIRCEGDWIKSEHLPPLGDANADAPHPHPGEEREWILDALQRHRFRRGEAARYLGISRKTLYNKMRVYGLPLQPRERS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Incorporation of the H-T-H motif domain in the designed protein should not interfere with its overall stability and folding.2. The designed protein should have ATP binding, sequence-specific DNA binding, catalytic activity to facilitate regulation of DNA-templated transcription.3. The designed protein should have a binding site that is specific to the target ATP ligand. The designed protein sequence is ",
    "response#1": "HMGSLRVACYTAPALSFMSGVIQTFIADRPDVSVYLDTVPSQTVLELVSLQHYDLGISILAGDYPGLTTEPVPSFRAVCLLPPGHRLEDKETVHATDLEGESLICLSPVSLLRMQTDAALDSCGVHCNRRIESSLALNLCDLVSRGMGVGIVDPFTADYYSANPVIQRSFDPVVPYHFAIVLPTDSPPPRLVSEFRAALLDAL",
    "response#2": "HMGSLRVACYTAPALSFMSGVIQTFIADRPDVSVYLDTVPSQTVLELVSLQHYDLGISILAGDYPGLTTEPVPSFRAVCLLPPGHRLEDKETVHATDLEGESLICLSPVSLLRMQTDAALDSCGVHCNRRIESSLALNLCDLVSRGMGVGIVDPFTADYYSANPVIQRSFDPVVPYHFAIVLPTDSPPPRLVSEFRAALLDAIR",
    "response#3": "HMGSLRVACYTAPALSFMSGVIQTFIADRPDVSVYLDTVPSQTVLELVSLQHYDLGISILAGDYPGLTTEPVPSFRAVCLLPPGHRLEDKETVHATDLEGESLICLSPVSLLRMQTDAALDSCGVHCNRRIESSLALNLCDLVSRGMGVGIVDPFTADYYSANPVIQRSFDPVVPYHFAIVLPTDSPPPRLVSEFRAALLDAMGV"
  },
  {
    "reference": "MLPEEKNTFYITTPIYYPSGKAHIGHAYTTVAGDAMARYKRLKGYDVFYLTGTDEHGQKIQAKAKERGISEQEYVDEIAEGFQELWKKLEISNTDFIRTTQDRHKTSVEKIFEQLLEQGDIYLGEYEGWYSVSDEEYFTETQLEEVYKDENGKVIGGKAPSGNEVELVKEESYFFRMSKYADRLVEYYNSHPEFILPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVVYVWIDALSNYITALGYNTDNDTKFQKYWPADVQIVGKEIVRFHTIYWPIMLMALDLPLPKMVFGHGWILMKDGKMSKSKGNVVDPYMLIDRYGLDALRYYLLREVPFGSDGLFTPEDFVDRVNFDLANDLGNLLNRTVAMINKYFDGEIPAYQGNVTEFDQTLVDFKNNVVKEYEGSMDHMQFSVALNQLWSLISRTNKYIDETAPWTLAKDEDKRTELASVMTHLAENLRIIAVLLQPFLTRTPGEIFLQLGLQEENLKKWDSIYGYGEIPAGTTVVKKGTPIFPRLEAEVEVTYIQDEMKGSAPAPAKEAAEVEALETPQIGIEDFDKIDLRVAEVKQVDKVKKADKLLCFQLDLGEGKLRQVLSGIAEFYQPEELIGKKVIVVSNLKPVKLRGLMSEGMILSGEKDGKLSVIEASSALPNGAKVK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein contains novel tRNA-binding domains that confer a unique function or activity.2. The protein must exhibit the following characteristics: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation..3. The protein should have a fast turnover rate for ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-   methionyl-tRNA(Met).4. The ATP binding site should be stable and able to withstand changes in environmental conditions.5. A protein that contributes to methionyl-tRNA aminoacylation by functioning in cytoplasm with ATP binding, methionine-tRNA ligase activity, tRNA binding.6. The designed protein should contain at least one 'KMSKS' region, 'HIGH' region motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "MLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSHEKDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEVCGATYSPTDLINPVSVISGASPVEMESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGFPIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKASKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYTHGFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRSMAPLEIAHHDFVARVNADLVGKFVNLASRCAGFIGKRFDGKLADALPDPAQYARFVEALAPIREAYERNDPASAIRQTMALADEANKYIDDTKPWVIAKQEGADAQLQSVCTQGLNLFRLLVAALKPILPRTAAEAEAFVNRVNADLVGKVVNIASRCAGFINKRFDNRLSSELINQKLYNDLLSAREFVIDAFVSRDYARAIRQIMDCADKVNQYIDANKPWVLAKDESKLNEVHAICTMGINLFRILITYLKPVLPMMAKASEEFIR",
    "response#2": "IRIGTIVSAEAIKKSKKLLKLQVDLGEEETRQIVAGLKESHEPEQLIGKQVAVLTNLAPAKLCGVESNGMVLAGVDAADNAILLQPEKETNPGPDATPMFIQIFISFLPILLIIGVFYFFMSQAQGGGNKVMNFGKSKAKLITKDTPKTTFADVAGADEAVEELYEIKDFLQHPAKYQAVGAKLRVGKIIEVKDHPNADKLYVVKVDLGDEVRTLVAGLKKYYKPEELLNRYVVVVANLEPKKLRGIGSQGMLLAADDGERVALLMPDKEVKLGADLAGRRAILKVHSAGKPFDDKAYEARDFSAAVRHIMRRADEVNELIHELAPWEIARDETRERELHRACSLGIQMFYLLSCYLKPILPRTAAQIEDFLNLGELSWQKQQAGQPLSDTLLPPGHVINPYQHLMTRIDPKQHIAAVFGGDRRRSRMLTLDDFVARVNSDLVGKYINIASRCAGFISKRFGGKLVSGEDYRLLQQMVDEHFAGWQPGVIEAAYEARDFSAAVRHIMRRADEVNELIHELAPWEIARDETRERELHRACSLGIQMFYLLSCYLKPILPRTAAQIEDFEAVLREKGLPAPADWTASRTEKTAQDSGATAESTPAEDAPVAEAPAAETDAPAEDTAPEAEEEK",
    "response#3": "MLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSHEKDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEVCGATYSPTDLINPVSVISGASPVEMESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGFPIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKASKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYTHGFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRVNSDLVGKVVNLASRVGKFASRTGLAPSYPEDGGLFQAAAAKGDEIASAYEDGEFSKAMRLIMELADAANPFVEHAKPWEMNKAPERQDELRDVCTVALNLFRQLAVYLAPVLPELAKKCGDLFKLAPEVADKSLIDSFLAKQDVIAELYEAREYGKAMREIMALADVANGFVADAAPWQLVKQDDKQEEAHQVCSNALNLFRILVTYLKPVLPRLAKDVEDFFRMELTQ"
  },
  {
    "reference": "MSEVSTNFLDYVDYTKPSFLHALVCIVIAPIIWNTIARLEFYTRFLTKITTSPYVGCYLLALWIFSFSLYRDFLFGKALDEQATHPELQSDLIRGVAAVLFVSGTTFVLSSMWALGVTGTYLGDYFGILMDKMVTGFPFNILDNPMYIGSTMCFLAHSLWRASPAGLVLTVVVYAVYRVALLFEEPFTAYIYAQREQKRKSGKAAAAPKAQKAKPKPKNKNKSKKN",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should contain Cytoplasmic topological domains.2. The designed protein must be optimized for phosphatidyl-N-methylethanolamine N-methyltransferase activity.3. The S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand.4. The protein must exhibit the following characteristics: Uses S-adenosylmethionine (S-adenosyl-L-methionine, SAM or AdoMet) as the methyl group donor for the methylation of phosphatidylethanolamine (1,2-diacyl-sn-glycero-3- phosphoethanolamine, PE) to phosphatidylmonomethylethanolamine (1,2- diacyl-sn-glycero-3-phospho-N-methylethanolamine, PMME), PMME to phosphatidyldimethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N,N- dimethylethanolamine, PDME), and PDME to phosphatidylcholine (1,2- diacyl-sn-glycero-3-phosphocholine, PC), producing S-adenosyl-L- homocysteine in each step.. The designed protein sequence is ",
    "response#1": "MLNVTIKERLTDEFILDYLGVNYYCFAGHTDVVPVGRIDDWNTPPFEPIIKDNVLYGRGSADMKTAIACFVVAIKRLIELKGNLPGSIALLITSDEEGPAVNGTIKVIETLQKRNEKIDYCIVGEPSSIEKIGNTIKM",
    "response#2": "MQIPNLSRRQFVAATGLGAAALVAGCGSTSGSKSSGSKDKVTFWHSMVGANGERMKLLADEFNKKTDYKIVPTGVYKGTDYKTTVQAAISSGKAPDVVQTYEVGTPSFFLSGAVLPLDDLLAKDSYDPKDFDDSIVSVGTYKGKQYGVPLNTSAPVMFYNKDAFKKAGLDPNKPPKTWQELAEDAKKLKAAGQTYGAEFYVGTKWYEWYPFAANAGNEIISKDDGKSATGTLNTPENIETLQFQAGLIKGGPSKVEQIGFDDTQVAFASGKAAMIINGPWQVGGYKKAKINYGVAPLPFNEGEKPAALGGGVNMVIFKQSPKQKEAAAKFVEWFTNTENTAKWHQETQYLPARKSVVNDPEFKKDPAIGVAAAQIKGAKARPAIPQMTAIRNALELPVQNVMTGKKTPQQACDAAAKEANKILSEAGY",
    "response#3": "MAAPAAASGPRGGPLYPSEIQRLVSQRWDQCHPDVLSPEASLTLQQLEQFLGTCLSYALVSFHRTYYSLAGDRLMPHFLESTWTMLSGWLILVAGLAYGFPYDPFVRRMRRYFMFMLAATYCVGMVEFLFHICRGYFRYIFLGTPLAVISLLVVFPSFLALPNLGLPCRSLGVFFLLVMTIPRVQCLTLPMVTSLGMGWVLWGCTDLGALMMAEWEEQKRQNIKEKRKSKKTKKK"
  },
  {
    "reference": "MEQLSSANTLFTLELFHTLKESSPTGNIFFSPFSISSSLAMVFLGAKGSTAAQLSKTLHFDSVEDIHSCFQSLTAEVSKLGASHTLKLANRLYGEKTYNFLPEFLASTQKMYSADLAAVDFQHASEDARKEINQWVKGQTEGKIPELLAKGVVDSMTKLVLVNAIYFKGIWEEQFMTRETINAPFRLNKKDTKTVKMMYQKKKFPFGYISDLKCKVLEMPYQGGELSMVILLPEDIEDESTGLKKIEEQLTLGKLHEWTKHENLRNIDVHVKLPRFKMEESYILNSNLCCLGVQDLFSSGKADLSGMSGSRDLFVSKIVHKSFVDVNEQGTEAAAATGGIIQVLCEKMPTPQEVFTVDHPFLFFIRHNPTANMIFFGRVCSP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The ideal protein would be one that plays a critical role in negative regulation of interleukin-1 beta production, regulation of protein catabolic process through serine-type endopeptidase inhibitor activity within extracellular region, cytoplasmic ribonucleoprotein granule.2. The protein must exhibit the following characteristics: Regulates the activity of the neutrophil proteases; During inflammation, limits the activity of inflammatory caspases CASP1 and CASP4 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation.; Forms only a stable complex with CTSG/Cathepsin G. The designed protein sequence is ",
    "response#1": "IVGGHEVTPHSRPYMASVSFEGHHYCGGFLIHTHWVVSAAHCFSDRDPSMGLVVLGAHALLAPEPTQQTFSIAAAVSHPDFQPATQANDICLLRLNGSAVLGPAVRLLRLPRRNAKPPAAGTRCHVSGWGFVSDFEEPPPGLMEVEVRILDLSVCNSSWQGQLNPAMLCTHSGDRRRRGFCSADSGGPLVCGRRAHGLVSFSGLWCGDPKTPDVYTQVSAFVTWIWDVVR",
    "response#2": "IVGGHEVTPHSRPYMASVSFEGHHYCGGFLIHTHWVVSAAHCFSDRDPSMGLVVLGAHALLAPEPTQQTFSIAAAVSHPDFQPATQANDICLLRLNGSAVLGPAVRLLRLPRRNAKPPAAGTRCHVSGWGFVSDFEEPPPGLMEVEVRILDLSVCNSSWQGQLNPAMLCTHSGDRRRRGFCSADSGGPLVCGRRAHGLVSFSGLWCGDPKTPDVYTQVSAFVTWIRANS",
    "response#3": "IVGGHEVTPHSRPYMASVSFEGHHYCGGFLIHTHWVVSAAHCFSDRDPSMGLVVLGAHALLAPEPTQQTFSIAAAVSHPDFQPATQANDICLLRLNGSAVLGPAVRLLRLPRRNAKPPAAGTRCHVSGWGFVSDFEEPPPGLMEVEVRILDLSVCNSSWQGQLNPAMLCTHSGDRRRRGFCSADSGGPLVCGRRAHGLVSFSGLWCGDPKTPDVYTQVSAFVTWIHAN"
  },
  {
    "reference": "MGSVPFSTARQVCPMATSPLNLAELEPVALEKTLAELGAPAFHGRQIFAWIHRRGVTRFEAMTDLPAGLRQALAERFAIYTPQLERVDQSTDGTTKFLLRLADDRRIESVYIPDTPAQTFCISTQVGCAMGCAFCLTGKMGLVRNLTAGEIVGQVRVLAHHTGLAGIAFNIVLMGMGEPLHNYDATMQALRILADPRGLGVHPRRVTLSTVGLIPALERLATEPLMPNLAISLHATTDPARTALVPLNRRYDLEALLDACRRFPVRRRARITFEYVLLAGVNDTPDDARRLVRLLDGIRAKVNLLPLNEAPGIPFRRPDDARVNAFAKILADKGVRVLVRRSRGRDIRAACGQLIVEGGRKSAAQQLADRMAPAADAAPR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have rRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding within cytoplasm to regulate rRNA base methylation.2. The S-methylcysteine intermediate, Proton acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "DDNRITLCLSTQVGCPLDCKFCATGLMGYKQNLTSGEIIDQYLLASKYFNKKITNIVYMGMGEPLLNYECSISSLKIFNSELISNIHLKHITVSTSGIVPNIYKLADSGLNIRLALSLHSCFNEIRNEIMPINKKYPLAELIEASKYFTNKTGLKITIEYIMLKDVNDRDEDIKGLAKLFSQLPIKINIIPFNSLKHMNPEGLSLELKPTSKERIDYFVSKLKEKNIG",
    "response#2": "FTICVSTQVGCPLACAFCATGQSGFKRNLTASEIIGQVYLITKNFPGVTNIVYMGMGEPFLNYANVVKSINVLISKEGLNFGQRKISISTSGIPEKIRIFAAEDWQVRLAVSLNSADERIRSRLMPINKKIPLSRIKDAIKYYISRTGRRVTLEYIMIKDVNDTPDALVSLLDFCQGLLVNVNLIPFNSIGGSFKASEIKTVNLFLP",
    "response#3": "HKFGLSVCVTTQVGCNIGCSFCASGLLAKSRDLSSGEIVGQIMKVQRHLDQTKQGERVSHIVVMGIGEPFDNYDNMANFIRTVKDHKGLAIGPRHITVSTSGLAEKIYEFADSDLQVNLAISLHAPNNELRTRIMKINRAIPIEKLMKAIDYYLEKTNRRITLEYILLKDVNDRREHAVELAELIGDRRDLANVNLIPYNPVDEHSQYQRSEQESVRAFYDTLKKQGISCSVGQSKITN"
  },
  {
    "reference": "MTNYYFIGIKGTGMASLALILHDQGNKVLGSDIDKETFTQFPLVKAGIQITDFDPDLIKSNYVVVKGNAFSDDHPQVLAAKKVGAKLLTYPQAVEEVIRRHFSIAVSGAHGKTSTTSLLAHILSNFESTSYLIGDGVGKGVFSSKYFVVEADEYDRHFQPFTPDVAIITNIDWDHPDYYKSLDDVKSAFSEFADHVKKIIFAYGEDPEIKKIKPKTARILSYGFEKSDYFCANNIDKRTEGSYFSLSKGGRKLGDFFATIEGDHGILDTTAAIAVADYLGFSVDQIQKGLLTYQGAKRRFNISQVADLTIVDDYAHHPTEIKATIDAARQKFPNKRLVAVFQPHTYTRVNAFKDEYVNALKSADKVYITPIFGSLREKAGNAKSEDILDKLPNAGAIISESNAYDKLSAEHGSVLVFMGAGDIEKYEDALKKGFE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. We require a protein that can specifically bind to the metabolites produced in the peptidoglycan biosynthesis pathway.2. The protein must be able to UDP-N-acetylmuramate-L-alanine ligase activity. The designed protein sequence is ",
    "response#1": "VTGSNGKSTVVSLLASLAKAIGVNAALAGNIGEPILNLLHRENVYNSQLANQPDIVIVELSSFQLETLSSMHAIAASVLNLSDDHLDRHKTLANYQAIKQSIYPQAKIAVVSREDQASNTLVAAQEIISFGLNKPEQDCFGLQAIDNKMVMMFGEQALISIDELPLAGMHNALNYMAALAIGVTGTNGKTTTTYLIEQILRDQGADRI",
    "response#2": "VGGTHGKTTTTTMVATLLDHGGVDPTVINGGIIHAYGSNARVGQGDWMVVEADESDGTFNRLPATIAIVTNIDPEHMEHWGTIENLRQGFLDFVSNIPFYGLAVCCTDHPEVQTLVGKITDRRVVTFGFNAQADVRAVNLHYRAGVACFDIALQSEDILIEGCTLPMPGDHNVSNALAAVAVN",
    "response#3": "VTGTNGKTTTTYLIEQILRDQGFITGLMGTIQVKIGSEYYGVERTTQEALDIQKNLAAMREKDTDYCVMEVSSHALDFGRVKGIPFRSGVFTNLTQDHLDYHKTMDNYRLAKAQLFSRMGNDFVQDEKQRSYAILNVDDEASETFQRLTSAEVITYGIEKECDVRASNIRITAEGTEFTLTTFEGTENIRMKLVGKFNVYNALGAIAAN"
  },
  {
    "reference": "MPDILTASQMRALETAAFNSGAVTGLELMERAGQSVIDALFSSKAELATGEHRAVILCGPGNNGGDGFVIARLLFVLGWQVRVYLYADPEKMPRDAHANHDSWVDLVPECTQRISFPSVLPAEAERFGDEAFGNGEVDVIVDALFGIGLNRPLSGLHPILATCHANRHEAYFVAVDVPSGLGENGPLEAADWSVFPADLTVTFHRPKQAHQNGLSFCGKIMVQYIGL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. The designed protein sequence is ",
    "response#1": "LSAKAAAALDRELMSTGAFSIDQLMELAGLSVSQAVARVHPTKQGRRVLVAVGPGNNGGDGLVAARHLFHYGYQPAIYYPKRPKNDLYQRLVKQCEDLEIPFVDDFFAALESTDHVVDAIFGFSFSGEVREPFPAVINAMAETKVPVTSVDAPSSWDINEGPPKSGVGSNFHPNVLVSLTAPKPLVKYFKGRHFVGGRFA",
    "response#2": "LSAKAAAALDRELMSTGAFSIDQLMELAGLSVSQAVARVHPTKQGRRVLVAVGPGNNGGDGLVAARHLFHYGYQPAIYYPKRPKNDLYQRLVKQCEDLEIPFVDDFFAALESTDHVVDAIFGFSFSGEVREPFPAVINAMAETKVPVTSVDAPSSWDINEGPPKSGVGSNFHPNVLVSLTAPKPLVKYFKGRHFVGGRFR",
    "response#3": "LSAKAAAALDRELMSTGAFSIDQLMELAGLSVSQAVARVHPTKQGRRVLVAVGPGNNGGDGLVAARHLFHYGYQPAIYYPKRPKNDLYQRLVKQCEDLEIPFVDDFFAALESTDHVVDAIFGFSFSGEVREPFPAVINAMAETKVPVTSVDAPSSWDINEGPPKSGVGSNFHPNVLVSLTAPKPLVKYFKGRHFVGGRFR"
  },
  {
    "reference": "MAHKKGQGASRNGRDSESKRLGLKVGAGQRVSTGSILVRQRGTKWHPAVNVGRGKDDTLFALVDGIVVMKKTDRTYVSVIPQA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be optimized for stability and solubility when expressed in ribosome where it will be involved in translation. The designed protein sequence is ",
    "response#1": "ERVRLESFNHELLTSACRKISDTIQNSDATKLSVVSLPTDKRIYCVLRSPHVDKDSREHFELRVHKRVIEIYYDPTVKCRIISKFRITVG",
    "response#2": "GRVRLESFNHELLTSACRKISDTIQNSDATKLSVVSLPTDKRIYCVLRSPHVDKDSREHFELRVHKRVIEIYYDPTVKCRIISKFRITVR",
    "response#3": "MPNSRP"
  },
  {
    "reference": "MVSQFAIEVRNLTYKFKESSDPSVVDINLQIPWNTRSLVVGANGAGKSTLLKLLSGKHLCLDGKILVNGLDPFSPLSMNQVDDDESVEDSTNYQTTTYLGTEWCHMSIINRDIGVLELLKSIGFDHFRERGERLVRILDIDVRWRMHRLSDGQKRRVQLAMGLLKPWRVLLLDEVTVDLDVIARARLLEFLKWETETRRCSVVYATHIFDGLAKWPNQVYHMKSGKIVDNLDYQKDVEFSEVVNAKVNGQVAFENDNNKVVISKVNSLHPLALEWLKRDNQIPDKEIGI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have ATP hydrolysis activity within CCR4-NOT complex, cytoplasm to regulate regulation of transcription by RNA polymerase II.2. The designed protein have ABC transporter domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "LSYNTLCQHYATPKMYKFTPSWALQWDYRKNLLEKEVLNYNTDIVCMQEVETKTFQEFWLPVMTANGYKGYFFSKTRSKTMSETDSKKVDGCATFFKNDKFSLIHKQNFEYNSVCMGSDKYKKTKDLFNRFMNKDNIALISYLQHKESGEKIAVVNTHLHWDPAFNDVKALQVGILLEELQGIIKKYRHTNSNEDIKNSSIVVCGDFNSVKDSAVYQLFSTGASKGHEDMNGRDYGKFTEDGFHHPFKLKSAYEAVGELPFTNLTPAFTDNIDYIWYSTPTLQVKGLLGGVQ",
    "response#2": "LSYNTLCQHYATPKMYKFTPSWALQWDYRKNLLEKEVLNYNTDIVCMQEVETKTFQEFWLPVMTANGYKGYFFSKTRSKTMSETDSKKVDGCATFFKNDKFSLIHKQNFEYNSVCMGSDKYKKTKDLFNRFMNKDNIALISYLQHKESGEKIAVVNTHLHWDPAFNDVKALQVGILLEELQGIIKKYRHTNSNEDIKNSSIVVCGDFNSVKDSAVYQLFSTGASKGHEDMNGRDYGKFTEDGFHHPFKLKSAYEAVGELPFTNLTPAFTDNIDYIWYSTPTLQVKGLLGGVP",
    "response#3": "LSYNTLCQHYATPKMYKFTPSWALQWDYRKNLLEKEVLNYNTDIVCMQEVETKTFQEFWLPVMTANGYKGYFFSKTRSKTMSETDSKKVDGCATFFKNDKFSLIHKQNFEYNSVCMGSDKYKKTKDLFNRFMNKDNIALISYLQHKESGEKIAVVNTHLHWDPAFNDVKALQVGILLEELQGIIKKYRHTNSNEDIKNSSIVVCGDFNSVKDSAVYQLFSTGASKGHEDMNGRDYGKFTEDGFHHPFKLKSAYEAVGELPFTNLTPAFTDNIDYIWYSTPTLQVKGLLGGVV"
  },
  {
    "reference": "MGALRTPAAAADWLRQRGAGTLCVDSRAVHPGDAFFAWPGAVTDGRRFVGGALQRGAAACLVDDDGAAAFNFNTEARVIRYAGLKAAAGPIADLHYGSPSKALKVLAVTGTNGKTSTAWWLAAALSRLPAPAFGSCGVVGTLGAGVFPNVGEHGLTTPDALALQHQLRAFVDAGATCCAMEASSVGLVEHRLDGTAIDVALFTNLTQDHLDYHGTMEAYWRAKALLFDWPTLSAAVVNLDDPRGPELAQHVRERGLDVWTVALGHAARLVAKDLHHGARGLDFTVAEEDREVTLNTALVGRYNVSNVLGVIGALRALGVSLEQAVGACAALPPVPGRMERIEAEATGPMAIVDFAHTPDALEKALTALRPLAAHRGGQLWCVFGCGGHRDRAKRPLMAAVVERLADRPVLTSDNPRDEEPEAIIRDMTAGLRHAAVVMIEPDRARAIALALRQAAAGDVVLIAGKGHETEQEINGVRHHFSDQEQARAALRARAGGVQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein requires Mg(2+) to activate a critical catalytic site.2. The protein contains novel Mur ligase N-terminal catalytic, Mur ligase C-terminal, Mur ligase central domains that confer a unique function or activity.3. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.4. The protein's functional design necessitates the inclusion of a Meso-diaminopimelate recognition motif motif.5. The designed protein should be able to compete with endogenous proteins or inhibitors for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP, meso-2,6-diaminoheptanedioate binding.6. The desired protein should be able to perform ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate The designed protein sequence is ",
    "response#1": "MNLKHIIDHVEVLELTGSLDAEVEITGVTTDSRAVRRGQLFAALPGTRVHGAAFIPMALEKGAGAILTDADGAALAADALAASDAALIVAEDPRAALAQTAVVWGNPSRRLQLVGVTGTNGKTSITFLLAQILERYGKKCGLIGTIANYIGDERLEAQNTTPDSYILQRYFAQMVDAGCEYCVMEVSSHALHQHRTGGIFFDTGVFTNLTQDHLDFHKTMENYFAAKRRLFTEYLRPGSYAVVNWDDPAGQEMAAMTSARTIRYGMEQDADVRAENIRITAKGTTFLLVTPRGEMETELKMTGRFSVYNVLAAIGAVLALDIPLKTIRAAVAGVKAVAGRMQLAATRKNGAAVYVDYAHTPDAIETALKALRPHVMGRIVIVFGAGGDRDTTKRPLMGAAARAHADVLYVTDDNPRSEEPATIRAAVLAACPDANEVGDRAEAILLGVDALGPGDALLIAGKGHETYQILKDRTIHFDDREAAREALAREKE",
    "response#2": "MKLRELIGIIEYPIAGINISGIEFDSRKVEVGSLFVAVKGYKADGHDYIDKAVASGASAVICEEAREKYGEISGIEFDSRKVEVGSLFVAVKGYKADGHDYIDKAVASGASAVICEITGTNGKTTIATLLYNLYSGLGYQVGLISTVVNKICERDVPATHTTPDPIRLNALLREMIDEGCSHCFMEVSSHAIHQHRTSGLRFAGGVFTNITHDHLDYHKTFKEYIAVKKAFFDGLPKTAFALTNLDDKNGEVMLQNTVARKRSYALKTMADYRAKVLENQFSGLLMTVQGIELWTRLIGDFNAYNLLAVFAVAKLLGVDEEVVREGIKTAEVPGRLERYESPSGAFFFVDYAHTDDALHNVLSAMRALNPRKIITVFGCGGDRDKTKRPRMGLAAANYSDHLIVTSDNPRNEDPSSILNDIIKGIPPETSFEVIADRAEAIKKAVKMAQKNDVVLVAGKGHEDYQIIGKKKFHFDDREEAKKALKVK",
    "response#3": "MQLRELVGGVAVEVVGAGDVEITGLAVDSRDVKPGFLFAALPGARVHGGEFIQYALRMKAGAILTDAKGAEIAADELAASDAAVIVAQDPRQALAQTAAVFHGHPSRDMTTVGVTGTNGKTSITFLLAQILERYGTRAGLIGTVGVTGTNGKTSITFLLAQILERYGRRCGVIGTVNYRYAGRSLPTAGFTTPDPIELQALLRDMRDAEVTHVAMEVSSHALDQWRVRGVDFEVAVFTNLTRDHLDYHGTMAAYAAAKARLFEGLGSGASAVVNGDDPAAAELVGAGAGAVTVGLSQDADVRLVDHELAADGTEGVLRTGGSTIPVRSRLVGRHNVHNVLAALATTLALGIAPADAVAGIAGFPGVPGRLERVDNGRGVMALVDYAHTGDALDKVLVTLAALAPRRMITVFGCGGDRDRSKRPVMGEIASRRSDLAVVTSDNPRTEDPDAIIAEVCAGVARVHRRPLTRAEAASGAKGYIVIPDRREAIAFAADVSRGGDLLLVAGKGHEPYQEVDGVVHPFDDREVARAALARRP"
  },
  {
    "reference": "MGIYDIFDDESARSISGMSIDEMAKKYGTPVIIYSRARIVSNIRRIREAYENRVRMLYSVKANDNPRIIEIMHQESIGSDSASPMEIMMSIFSGIPPEDILYSPNNASEYDLNFALDRGIAINFNTFTQYRKMREKPERISFRINPGFGMGEFAGTTTGGARTKFGIDPDAAILAYRKAREDGIREFGIHMMIGSNNRDHVKIAEAYSNFFRIADRIGREAGVSFQFADVGGGLGIPYVQGENELDIAALGSAVLKEFDRYHFGDLVLEPGRYLVGDAGIIVGTVNDVHNGFAGTDIGMNLNIRPALYGARHTIIPVGERVEGEKITVTGQICENTDRIGDTAWRLSEGDRIMVLDAGAYVYSMSSRYNGRPRPPEIMIMEDGKDVMIRRREDFSDFIATVV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Specifically catalyzes the decarboxylation of meso- diaminopimelate (meso-DAP) to L-lysine..2. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate, pyridoxal 5'-phosphate binding.3. We need a protein that can perform H(+) + meso-2,6-diaminoheptanedioate = CO2 + L-lysine with high efficiency.4. The protein should be optimized for activity in L-lysine biosynthesis via DAP pathway, Amino-acid biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "FWVYDADIIYKKINLLRKFDVIRFAQKSCSNINILRLMKNKNVKIDAVSLGEIERALLSGFKPSTNEIIFTADILDEETLLKVAKYKIPVNAGSLDMLKQLGKISPGHHVWLRINPRFGYGHSKKTNTGGENSKHGIWHPEQAIPIIKKYKLKLIGLHMHIGSGVNYLHLKKVSQAMIKYAFQLNQKISSISVGGGLPIPYKFNDQPIDIKKYFMLWDIARKKISKFLGEKIELEIEPGRFLVAESGILISKVWATKKMGNKNFVLVDVGFNDLMRPTMYGSYHHISVIYGDDRKMNEKETIDTVVAGPLCESGDIFTQKEGGTVQTRKLPTIKVGDYLIFHDTGQA",
    "response#2": "FWVYDADIIYKKINLLRKFDVIRFAQKSCSNINILRLMKNKNVKIDAVSLGEIERALLSGFKPSTNEIIFTADILDEETLLKVAKYKIPVNAGSLDMLKQLGKISPGHHVWLRINPRFGYGHSKKTNTGGENSKHGIWHPEQAIPIIKKYKLKLIGLHMHIGSGVNYLHLKKVSQAMIKYAFQLNQKISSISVGGGLPIPYKFNDQPIDIKKYFMLWDIARKKISKFLGEKIELEIEPGRFLVAESGILISKVWATKKMGNKNFVLVDVGFNDLMRPTMYGSYHHISVIYGDDRKMNEKETIDTVVAGPLCESGDIFTQKEGGTVQTRKLPTIKVGDYLIFHDTGRDHCFAFQLNQKISSISVGGGLSFWVYDADIIYKKINLLRKFDVIRFAQKSCSNINILRLMKNKNVKIDAVSLGEIERALLSGFKPSTNEIIFTADILDEETLLKVAKYKIPVNAGSLDMLKQLGKISPGHHVWLRINPRFGYGHSKKTNTGGENSKHGIWHPEQAIPIIKKYKLKLIGLHMHIGSGVNYLHLKKVSQAMIKYAFQLNQKISSISVGGGLPIPYKFNDQPIDIKKYFMLWDIARKKISKFLGEKIELEIEPGRFLVAESGILISKVWATKKMGNKNFVLVDVGFNDLMRPTMYGSYHHISVIYGDDRKMNEKETIDTVVAGPLCESGDIFTQKEGGTVQTRKLPTIKVGDYLIFHDTGRDHCF",
    "response#3": "FWVYDADIIYKKINLLRKFDVIRFAQKSCSNINILRLMKNKNVKIDAVSLGEIERALLSGFKPSTNEIIFTADILDEETLLKVAKYKIPVNAGSLDMLKQLGKISPGHHVWLRINPRFGYGHSKKTNTGGENSKHGIWHPEQAIPIIKKYKLKLIGLHMHIGSGVNYLHLKKVSQAMIKYAFQLNQKISSISVGGGLPIPYKFNDQPIDIKKYFMLWDIARKKISKFLGEKIELEIEPGRFLVAESGILISKVWATKKMGNKNFVLVDVGFNDLMRPTMYGSYHHISVIYGDDRKMNEKETIDTVVAGPLCESGDIFTQKEGGTVQTRKLPTIKVGDYLIFHDTGQACPVP"
  },
  {
    "reference": "MTHTKYIFVTGGVVSSLGKGIISSSIGKLLQARGYNITIQKFDPYINIDPGTLNPYEHGECYVTVDGMETDLDLGHYERFTGIQTTKANSLTTGRIYKAVIDKERHGDYLGKTIQVVPHITDEIKRNVKLLGEKYHYDFVITEIGGTIGDIESAPFMEAIRQLRWELGKNAINVHLTYVPYLRAAGELKTKPTQHSVKELQSVGIQPDILVLRTEKHLSDEIRHKVAAFCNVDYDCVIQSEDLPSIYEVPVNMQNQGIDTAILRKMNMEVGPTPKLGPWKSFLDRRNKATKEVHIGLVGKYDLQDAYKSIRESLSQAGVYNDHKTVLTFINSEDITEDNVAQKLTGQDGIVICPGFGHRGIEGKIIAAHYTRTHDIPTFGICLGMQMMVIEFARNVLGYTDANSKEMDEKTPHNVIDIMEEQKDITNMGGTMRLGAFDCILRQGSHVFNIYQKEHIQERHRHRYEFNSDYIKEYEQKGMQCVGRNPESNLVEIVEIPELKWYIGTQFHPEYQSTVLHPHPLFLDFIKTAIINK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be optimized for CTP synthase activity, glutaminase activity, ATP binding activity in order to improve 'de novo' CTP biosynthetic process.2. A protein should be able to manipulate the activity of enzymes in the CTP biosynthesis via de novo pathway, Pyrimidine metabolism pathway, either by activating or inhibiting their function.3. The designed protein must have a Nucleophile that is structurally stable and resistant to denaturation.4. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen5. The protein contains novel Glutamine amidotransferase, CTP synthase N-terminal domains that confer a unique function or activity.6. The designed protein should have a high specificity for ATP, CTP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "LLDSYKSLNEALLHGGIAHRVKVTLDWIDSEQFEKNPATLDEKLGGVDAILVPGGFGERGSQGKIEAIRYAREKGIPFLGICFGMQMAVIECARNLAGLSEASSTEFGRTAEPLVGLMTEWARGNERLRRSEGGELGGTMRLGAYPATLVEGSRAAEVYGITSIRERHRHRYEVNIHYKDQLEKAGLRFSGLSPDGILPEVVEYTDHPWFIAVQYHPELKSRPFAPHPLFAGFVAAALA",
    "response#2": "LHDAYLSIVESLGHAGISADSEVKIRWVDAEQLTDDTVADALQGIHGILVPGGFGDRGIEGKICAIKYAREAKIPFFGICLGMQVAVIEYARNVAGLAGANSSEINSSTPYPVIDLLPDQKDIEDLGGTMRLGLYPCKIASETIAIREYGDELVYERHRHRYEFNNEYREVIESAGLIISGTSPDGRLVEMVELKDHPWFLAVQFHPEFTSRPNRPQPLFRGFVEAAAAL",
    "response#3": "VRVAVVGKYTALLDSYKSLNEALLHGGIAHRVKVTLDWIDSEQFEKNPATLDEKLGGVDAILVPGGFGERGSQGKIEAIRYAREKGIPFLGICFGMQMAVIECARNLAGLSEASSTEFGRTAEPLVGLMTEWARGNERLRRSEGGELGGTMRLGAYPATLVEGSRAAEVYGITSIRERHRHRYEVNIHYKDQLEKAGLRFSGLSPDGILPEVVEYTDHPWFIAVQYHPELKSRPFAPHPLFAGFIDAVANR"
  },
  {
    "reference": "MDFFPIFLDLRNQPCLVIGGGTTAARKIELLQQAGALIDVVAPELQDKLVLLQQARSLRYYPRTFQASDLQMQAYRLVIAATSDNAVNRDISECANALNIPVNVVDQPELCSFIVPSMLDRSPLQVAISTGGAAPVLARMLRAKLESVIPAAFGDLARLMAQFRDKTKRVFTNTAQRRDFWETVLSGHIAELVLSGQIAMAEQAVETLLHEKSTVHSTMGEVYLVGGGPGDPDLLTFRALRLMQKADVVLYDRLVSPSVLALVRRDAEHIYVGKRPNNHTLPQEEINLLLARLAKEGKKVLRLKGGDPFIFGRGGEEIETLMAEGVPFQVVPGITAALGTAAYAGIPLTHRDYAQSCIFVTGHLKDNSIDLNWQVLTQAHQTVVVYMGLKAISIFCAEMIKHGMSGEQPAALVQKATTADQKVIVGTLQTLPQLVEAENVNLQSLIIVGDVVKLHEKLKWFNPETMARKNASVM",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Ideal protein should have a binding affinity for key enzymes in the sirohydrochlorin from precorrin-2: step 1/1, Porphyrin-containing compound metabolism, siroheme biosynthesis pathway to enhance the overall efficiency of the pathway.2. The designed protein should be optimized for uroporphyrin-III C-methyltransferase activity, NAD binding, sirohydrochlorin ferrochelatase activity, precorrin-2 dehydrogenase activity activity in order to improve siroheme biosynthetic process, cobalamin biosynthetic process, methylation.3. For general function, the protein need meet that Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1; Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.; Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin4. The protein should be able to bind S-adenosyl-L-methionine ligand in a variety of conditions5. The Proton donor of the protein must be engineered to enable effective catalysis of NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin. The designed protein sequence is ",
    "response#1": "LPIFMKLRDRPCLVVGGGEIASRKVSLLEKAGASVTVVSPELHPTLAKSLAEGRIRHLASVFEPAQLDGAVLVIAATDDAEVNRAVSREAQARNIPVNVVDAPELCTFIVPSIVDRSPLLVAVSSGGTAPVLARMLRTRIETLIPATYGRLAAFAAEFREAVKQRFSTGQQRRIFWEDVFQGVIGEQVLSGQEEAARHAMQQVVLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDEVMNLVRRDAERIFVGKQSGHHCVPQEQINQILLQQAQSGKRVVRLKGGDPFIFGRGGEELEELAGYGIPFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHAKKEGQLDWANLAAEKQTLVFYMGLSQAGEIQQQLIQHGMPATTQVALVENGTSRHQRVVSGELSQLVVLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDEVMNLVRRDAERIFVGKQSGHHCVPQEQINQILLQQAQSGKRVVRLKGGDPFIFGRGGEELEELAGYGIPFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHAKKEGQLDWANLAAEKQTLVFYMGLSQAGEIQQQLIQHGMPATTQVALVENGTSRHQRVVSGELSQLGSVALVGAGPGDAGLMTLRGLRVLNEADVILHDRLVSADVLRLARRDAELIEVGKQAGNHHTTQDRIHELMLEHARAGKRVVRLKGGDPFVFGRGGEELEVLADAGIAFEVVPGITAAVACAAYAGVPLTHREHAQSLRLVTAHLKDSTDTLDWQALAQEKQTLAVYMGVSGLDLLRDKLIQHGRAASTPFALIENGSRREQRVVTGTLADLAERARGHQVQSPALLIVGEVAAL",
    "response#2": "MTYPLFADLSGRQVLVVGGGVVGERKAAVLLEAGARVAVGAPDLTPALLVWAEQGRVQWLRGAFDESWLDEAWLVVAATSDQALHQRIAALAEARRVFVNVVDDAEQSSFHVPAVIDRSPVTIAISSGGHAPMLARLLRERLETLIDPVVGPVATLLAQMRHRIRARLPDMALRRRFYERLLNGPVQSLLRRGQWALAEASTETEIAGNVAVVGAGPGDAGLLTLNALRAMNEADVVLHDRLVSEDVLRLGRRDATYIEVGKSATGQSTRQEDIHALMLEHARAGRRVVRLKGGDPFVFGRGGEELEFLRAHGIPYEVVPGITAAIACAAYAGIPLTHRDHAQAVRLVTAHCKDSLDTLDWAALAQERQTLAVYMGVAGLEGLRERLLAHGRAPTTPFAIIENGSRREQRVLNGTLADLPETARSHQVRSPALLILGEVAALAGDLGERASARRFYEGEIALVGAGPGDVGLLTLRGLQVMQQADVVLYDHLVSDETLDLVRRDAERICVGKRAGAHSVIQEETNRLLVELAQQGKRVVRLKGGDPFIFGRGGEELQVAAAAGIPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCRPDGSGLDWADLARARQTLAIYMGTMKAADISRNLIAHGREGNTPVAVISRGTRPDQQVQTGTLQQLEQLAQQAPLPALLVIGEVVELGAGPGDPGLLTLRGLRALNEADVILHDRLVSVEVLALARRDAERIEVAKQAGNHHTTQAQINALMLEHASAGRRVVRLKGGDPFVFGRGGEELEFLRSHGIPYEVVPGITAALACAAYAGIPLTHREYAQSVRLVTAHCQNSNDTLDWRALAQERQTLAVYMGTSELPVIRSRLLEHGRSAGTPFALVENGSRPEQRVVTGTLANLVEQASLHDVRSPALLIIGEV",
    "response#3": "MTYPLFADLSGRQVLVVGGGVVGERKAAVLLEAGARVAVGAPDLTPALLVWAEQGRVQWLRGAFDESWLDEAWLVVAATSDQALHQRIAALAEARRVFVNVVDDAEQSSFHVPAVIDRSPVTIAISSGGHAPMLARLLRERLETLIDPVVGPVATLLAQMRHRIRARLPDMALRRRFYERLLNGPVQSLLRRGQWALAEASTELEAALTAFFGVGAGPGDPDLLTIKALNELQKADVVLFDQLVSPEILALIRPEAECISVGKKAGAHSMAQEDINQLLVVKAQEGHRVVRLKGGDPFMFGRGAEELQVLKAAGIAYQVVPGITAAAGATAYAGIPLTHRDHAQSAVFITGHAKRDGQDPDWESLAKQRQTLVIYMGLMRSAHIQQQLLKHGLASSTPVALIERGTTAEQRVVRGNLSELSQLAEKVVSPSLMVIGEVLGGAVSHPEVLHRGNVAVVGAGPGDAGLLTLNALRAMNEADVVLHDRLVSEDVLRLGRRDATYIEVGKSATGQSTRQEDIHALMLEHARAGRRVVRLKGGDPFVFGRGGEELEFLRAHGIPYEVVPGITAAIACAAYAGIPLTHRDHAQAVRLVTAHCKDSLDTLDWAALAQERQTLAVYMGVAGLEGLRERLLAHGRAPTTPFAIIENGSRREQRVLNGTLADLPETARSHQVRSPALLILGEVAAGEIALVGAGPGDVGLLTLRGLQVMQQADVVLYDHLVSDETLDLVRRDAERICVGKRAGAHSVIQEETNRLLVELAQQGKRVVRLKGGDPFIFGRGGEELQVAAAAGIPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCRPDGSGLDWADLARARQTLAIYMGTMKAADISRNLIAHGREGNTPVAVISRGTRPDQQVQTGTLQQLEQLAQQAPLPALLVIGEVVEL"
  },
  {
    "reference": "MKKSLTYLLLLSLLYSCSTSQISNDKMANNESKGKCPLHFGSNQTSGLTTTNQGNTNRDWWPNQLDLTILRQNSSLSNPMGEEFSYAKAFKSLDYYALKKDIEVIMTESKDWWPADYGNYGGLFIRMAWHSAGTYRTGDGRGGTRAGQQRFAPQNSWPDNANLDKARRLLWPIKQKYGQKISWSDLMILAGNVALESMGFKTIGFAGGRTDVWEPQSNVYWGAEKKFLDDERYTEERELEQPLAAVQMGLIYVNPEGPNGEPDPIKAAVDIRETFARMGMNDEETVALIAGGHTLGKTHGAASGDNLGESPEGAGIEQQGMGWKSEYKSGKGEDAITSGLEVIWTEEPTKWNHGYFESLFENEWKLTKSPGGANQWVAKNPHKMFPDAFDSEIIHKPTMLTTDLSLLEDSAYRIISQKFYEDPVAFELAFAKAWFKLTHRDMGPKSTYLGPEIPKEEFTWQDPIPSRDHDLVSDKNIASLKNSIQNSKLTNQDMVTTAWASASTFRISDKRGGANGSRIRLEPQINWESNNPEQLKRILNSYIEIQQEFESNSNQKISIADLIVLGGCVAIEDAASKAGYDISVPFSAGRMDASSEETNIEGMEVLEPMADGFRNYQKADYTLTAEQLLVDKSQLLTLTAPEMTVLVGGMRVLNSNYDNSSIGVLTETPGLLNNDYFKNLCSMDYEWKAKSDKALQFDGYFRGTEEIKWTASRTDLIFGSNSELRAICEVYASDDSNEKFVTDFVNAWTKVMNLDRFEI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein have Plant heme peroxidase family profile domains with specific sequence motifs that are crucial for its activity.2. The heme b binding site should be located in a region of the protein that is accessible to the ligand.3. The Proton acceptor of the protein must be engineered to enable effective catalysis of AH2 + H2O2 = A + 2 H2O.4. A protein with heme binding, catalase activity, metal ion binding functions is required to enable hydrogen peroxide catabolic process, response to oxidative stress.5. Incorporate a signal peptide in the protein design.6. The protein must exhibit the following characteristics: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.. The designed protein sequence is ",
    "response#1": "TFARAWFKLLHRDMGPKVRYLGPEVPAEELIWQDPVPAVDYELFHDEVAELKNRILSSGLTVSELVKTAWASASTYRHTDMRGGANGARIRLAPQKDWEANEPEQLAKVLGVYEDIQSKLTVRVSLADLIVLGGSAAIEKAAKDAGFDVTVPFTPGRGDATEEQTDAETFDVLEPVSDGFRNYQKKEYSTSPEEMLVDKAQLLGLTAPEMTVLLGGMRVLGTNHGGTKHGLFTDRPGILTNDFFINLLDMRTEWKSIGFNQYEGRDRKTGEVIRTASRFDLLFGSNSELRALAEVYAQR",
    "response#2": "FGHYGPLFVRMTWHAAGTYRVQDGRGGAGRGQQRFAPTNSWPDNVGLDNARRLLWPIKKKYGKKLSWSDLIVYAGNRAQEQMGFKSAGFVFGRPDYWEPEEDIYWGAEAEWLGTRERYAGSNGDRTRLENPLAASTMGLIYVNPEGPEGNPDPLAAAIDIRETFGRMAMNDVETAALICGGHTFGKTHGNGDASVLGPEPEAAALENMGLGWRNPNETGNPNDKVASGLEVTWTHTPTKWDNSFLEILYSNEWELFKSPAGANQWRPKDNGWANSVPTADLKGRTHPSMLTTDLSMRFDPIYGEITRRTAAAA",
    "response#3": "AFARAWFKLTHRDMGPRTRYVGAAAPAEDLIWQDPIPEVKHPLIDANDIAGLKKQILASGISISDLVRTAWASASTFRGSDRRGGANGARIRLAPQKDWRVNNPQQLAKILAKLEGIQKAFNDAQSGGKQVSLADLIVLAGCAAVEEAARQAGFDVEVPFTPGRGDATPEQTDVESFAVLEPVADGFRNYVCGDSIPRPEDLLVDKAQLLNLTAPEMTVLVGGMRALAANWDGSQNGVFTQRPGQLTNDFFVNLLDFSTAWKATDDSKQLFTGTDRKTGAPKWTATRVDLIFGSNAELRALAEVYAADYGHYGPFFIRMSWHAAGTYRTADGRGGGGSGSQRFAPLNSWPDNVNLDKARRLLWPIKQKYGNKISWADLLVLTGNVALESMGLKTFGFAGGRKDIWHAEEDVYWGTEKEWLADNRYSGDRELEDPLAAVQMGLIYVNPEGPNGKPDPLASARDIRDTFGRMAMNDEETVALIAGGHTFGKAHGAGDPSHVGDDPEAADLETQGLGWLSSYGSGKGRDTISSGVDGAWTTNPTTWDNGYFDLLFSYEWELTKSAAGAYQWTPVDMAEEHMAPDAEDPSIRVKTMMSTADMALRMDPEYEKISRRYYEHKGEA"
  },
  {
    "reference": "MENTDAIQVSSFPLPPAQYYKLYTDENVRNNRAPKPPAPIQGTYQMFGQQFSTEDNIIRPLEAQGFKRLYPQHFDRRKELKKLNHSLLVNFLDLIDLLVHNPDSPQRAEKIEDLNLLFVHIHHLLNEFRPHQARETLRVMMELQKRQRQETTQRFQNHLEKVREMVNNAFASLPDPAESDRLNSTVEPMDTGDDGEAGKSRGEGCHPLDRLMCELVDKM",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors .; Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery2. A protein that can perform transcription coregulator activity in mediator complex to support regulation of transcription by RNA polymerase II. The designed protein sequence is ",
    "response#1": "GCCSSGCC",
    "response#2": "HXGSXGL",
    "response#3": "GHNHHHHHH"
  },
  {
    "reference": "MYTTEFLDLPPLLGIGGAVRLPGSKSISNRVLLLAGLSEGTTVVHDLLASDDTRVMLDALRQLGCDLVQTGDTVRITGLAGRLSVLNADLFMGNAGTAIRPLTAVLALLSASHGGLYTLSGVPRMHERPIGDLVDALRPLGCHIDCLGQEGYPPLRVSGGQLKLGQAIRVRGDVSSQFLTALLLALPLVSQQQDLTIEVEGELISKPYIEITLNLLARFGIQVQREGWERFVIPQGSRYQSPGEIHIEADASSASYFIAAGALAATEDPLRIEGVGADSIQGDIRFVEAAQAMGAQVASGPNWLEVGRGAWPLKAIDLDCNHIPDAAMTLAVMALYAQGTTRLRNIASWRVKETDRISAMATELRKLGATVVEGGDFIEVTPPAAWQHASIHTYDDHRMAMCFSLAALNPLAHTATPGQPISVRIEDPRCVGKTFPDYFETLFQVADTHPALIPVITVDGPSASGKGTLANNLATRLGYQFLDSGSLYRVTGLMVVEKGIDPDDHEAVTKIARQIGTHIPLHFEQHNVWLNGKEVSEDLRQEASGHMASRISAIPGVRQALVDLQLSFRALPGLVADGRDMGTVIFPDAALKVFLTASVKERAERRYKQLISKGFSANLEEICADLEARDLRDRTRAVSPLVPAPDALKLDNSALTSDQSADTVLKWWVDGWPHVKSVKPT",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. We require a protein that can specifically bind to the metabolites produced in the Metabolic intermediate biosynthesis pathway.2. The designed protein have Cytidylate kinase domains with specific sequence motifs that are crucial for its activity.3. The ATP, 3-phosphoshikimate binding site should be stable and able to withstand changes in environmental conditions.4. For general function, the protein need meet that Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.5. The designed protein should have dCMP kinase activity, CMP kinase activity within cytoplasm to regulate chorismate biosynthetic process, amino acid biosynthetic process, pyrimidine nucleotide metabolic process, phosphorylation. The designed protein sequence is ",
    "response#1": "RGRVRLPGSKSISNRVLLLAALAEGDTDIRDLLASDDVERMLDALRALGVHWTRRAGSDNYAVRGVGSAFPVKAAELFLGNAGTAFRPLTAALALCGGEYRLSGVPRMHERPIGDLVDALRQAGAAIDYLGNEGYPPLAIHPASVRAGGVLKVRGDVSSQFLTALLMALPLTGGETVIEVVGELISKPYIAITLALMERFGVAVRRDGWARFTVPAGARYRSPGTVYVEGDASSASYFLAAGAIGGGPVRVEGVGRDSIQGDVRFAEALAELGVRIDMGPNWIAARAPENGRLRAFDMDLNHIPDAAMTLAVAALFADGPCTLRNIASWRVKETDRIAAMATELRKVGATVEEGADYLKVAPPARLRVAAIDTYDDHRMAMCFSLVSLGGLRVRINDPKCVNKTFPTYFEGTVTLPGSKSISNRILLMAALAEGRTEIHGLLAADDTQVMLESLRTLGVQWTRDGDTVTVDGMGGRVPQAQADLFLGNAGTAMRPLAAALALMAARYGGDYQLRGVPRMHERPIRDLVDALRGLGCQVDCLAQAGYPPLRLTGPSSLHLDTPVRVRGDVSSQFLTALLMALPLVAQRDVVVEVEGELISKPYIDITLALLGRFGVRVQRDGWQRFTIPAGSRLQSPGRIHVEGDASAASYFIALGALAALGEPLRIEGVGSDALQGDVRFADAAAAMGADIRLEPNALLVQRGRWPLRGIEWDCIAIPDAAMTLAVMALFADGPSKLTGIGSWRVKETDRIAAMATELRKLGATVDEGPDWIRVEPLREWRPASIRTYDDHRMAMCFSLAALNPLGGHGAAPVRIEDPACVAKTYPDYFE",
    "response#2": "KAAGSVRLPGSKSISNRVLLLAALAEGETLVEDLLDSDDTRVMRGAIVALGVKTMEEGKALRVFGCGGRFPKLKADIFVGNSGLSIRTLVPAIVTSLSGCADVDAEVRLSGVPRMHERPIGDLVDGLKQLGACVEWLGQEGYPPLALKPALLGAERVSVRGNVSSQFLTGLLQAAPLVARDKPLTIDVEGELISRPYVEITLNLMERFGVIVQREGWNRFVVPAAQRYVSPGRIAVEGDASTASYFLAAGALGGGPVRVVGAGRKSIQGDVKFADALATMGAEITWGDDWIEARAPTSGKLKAFDLDLNHIPDAAMTLAVAALFADGPSTLRNIGSWRVKETDRIAAMATELRKLGATVEEFPESITVTPPAGLNPAVIDTYEDHRVAMCFSLATLGGPYVRINDPRCVNKTFPEYFNLLK",
    "response#3": "KAAGSVRLPGSKSISNRVLLLAALAEGETLVEDLLDSDDTRVMRGAIVALGVKTMEEGKALRVFGCGGRFPKLKADIFVGNSGLSIRTLVPAIVTSLSGCADVDAEVRLSGVPRMHERPIGDLVDGLKQLGACVEWLGQEGYPPLALKPALLGAERVSVRGNVSSQFLTGLLQAAPLVARDKPLTIDVEGELISRPYVEITLNLMERFGVIVQREGWNRFVVPAAQRYVSPGRIAVEGDASTASYFLAAGALGGGPVRVVGAGRKSIQGDVKFADALATMGAEITWGDDWIEARAPTSGKLKAFDLDLNHIPDAAMTLAVAALFADGPSTLRNIGSWRVKETDRIAAMATELRKLGATVEEFPESITVTPPAGLNPAVIDTYEDHRVAMCFSLATLGGPYVRINDPRCVNKTFPEYFS"
  },
  {
    "reference": "MAKFIFVTGGVISGLGKGIAAASIGLLLKNKGLKIAVEKFDPYLNVDPGTMSPYQHGEVFVTEDGGETDLDLGHYERFIGTNITKNCSFSMGQIYSEIIKKERKGSYLGGTVQVVPHVTDIIKNKIKALAESSKADVIITEIGGTVGEIEAEVFLEAIRQFKTEMGRENVMFIHLTLVPYLKISDELKTKPTQHSVKELQKHGINPDMLLCRADRHIPEELLNKIAMFCNIDTNYVFEGIDVDTIYDLPINFHDEEMEERICERLGIKTERKDIDEWKALVDNFKNPDKEVNIGLVGKYVALQDAYLSVSESLKHAGAHHKCKVNIEWINSEDIEANGVEKYLSKLDGIIVPGGFGNRGIEGKISAAKYAREHKIPYLGLCLGMQIAVIEFARNVCGLSNANSSEFGTYEHQVIDLMEAQVGISNKGGTMRLGSYPCTLKEGTKAHRAYGEDKINERHRHRYEFNNNYRDIMNNNGMILAGTSPDNMLVEIIELSDHPWFVASQFHPEFKSRLCEPHPLFRDFVGATIVK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be optimized for activity in Pyrimidine metabolism, CTP biosynthesis via de novo pathway, CTP from UDP: step 2/2 pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The designed protein should be optimized for CTP synthase activity, ATP binding activity in order to improve 'de novo' CTP biosynthetic process.3. The designed protein is the optimization of the active site to achieve high catalytic efficiency for H2O + L-glutamine = L-glutamate + NH4(+).4. The designed protein must have at least one Glutamine amidotransferase domain.5. The designed protein should have a binding site that is specific to the target CTP, L-glutamine ligand.6. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.. The designed protein sequence is ",
    "response#1": "LPDAYLSVSEALHHAGLKHGLGVHIDWVDSESLERGRDWERVEEADGIVIPGGFGHRGIEGKVRAARFARENGRPYLGLCLGLQVMVIELARHALGNDDPNSTEFNPATKFPVIDLLPEQVNITDMGGTMRLGAWPCRVLPSTRAAAAYGTDLIHERHRHRFEVNPSFLPLLKEAGLVVSGVYTERDLVDIAEIRDHPYMVGSQFHPEFKSRPLRPHPLFEAFLQDAYKSIYESFIHAGAINECKVKVVPIHSEQLEGEAHNVAKFLKDKFDGILVAPGFGERGIEGKIEAVRYVRENKIPFFGICLGMQCAVVEFSRNVANIKEAGSSEVDELSQAPVIDLMEEQKNIVTKGGTMRLGAYDCRLRPGTRVAEAYGKTDISERHRHRYEFNNAYLEQLTEAGLIIAGVNPKTDLVEVIEIEDHPYFVGVQYHPELKSTVENPHPLFVSFVDDVADELGVTIGLVGKYNELQDAYKSIYESFIHAGAINECKVKVVPIHSEQLEGEAHNVAKFLKDKFDGILVAPGFGERGIEGKIEAVRYVRENKIPFFGICLGMQCAVVEFSRNVANIKEAGSSEVDELSQAPVIDLMEEQKNIVTKGGTMRLGAYDCRLRPGTRVAEAYGKTDISERHRHRYEFNNAYLEQLTEAGLIIAGVNPKTDLVEVIEIEDHPYFVGVQYHPELKSTVENPHPLFVSFVRIAVVGKYTDLPDAYKSVVEALIHGGITNDAKVEIEWIASDRFSDIESAGELLGGVDGLLIPGGFGERGIEGMVQAVRWAREHELPFFGICLGLQVAIIEFARNVCELPDSNSTEFEPECETPVIALMTSQHDVENLGGTMRLGAYPARLRKGSRVAQVYGSTEISERHRHRWEVNNAYRDVLAEFGMRLSGQSPDGGLVEIIELPNHPWFIGCQFHPELKSRPTRPHPLFERF",
    "response#2": "MPKYIFVTGGVTSSLGKGIISASLGKLLQARGFRVTIQKFDPYINIDPGTLNPYEHGECYVTDDGAETDLDLGHYERFLNICTSQANNVTTGRIYNNVITKERKGEYLGKTVQVVPHITDEIKRNIFLLGESGEYDFIITEIGGCVGDIESLPFLEAVRQVRWDLGPNNALVVHLTLIPYLKAAKELKTKPTQHSVKQLLEAGIQPDVLACRTERPLPLELRKKLALFCNVHLNSVIEAIDADSIYDVPLLMKKEKLDERVLNALHLPGTSPDLSQWQEFLGRLRHPDEELPDSYKSINEAFVHAGAQNDCKVNVKWISSEEINPETCEDILAGLDGILVAPGFGERGMEGKIEAIRYVREQKIPFFGVCLGMQCAVIEFARNVLKLHGASSTELDPNTRHPVIDLMEEQKKVTEKGGTMRLGAYACRLKKGSKAYNIYGESIIHERHRHRYEFNNKYLNDIEEAGLKAVGVNPESGLVEVVELKDHPWFIGVQFHPELKSTVLNPHPLFVKFVFVAAAKAALSR",
    "response#3": "TASSLGHLLAARGLVVTMQKLDPYLNVDPGTMNPFQHGEVFVTDDGAETDLDIGHYERFLGRNLDAGANVTTGQVYSTVIAKERRGEYLGDTVQVIPHITDEIKARMRAQTNPVEGPVPDVIITEIGGTVGDIESQPFLEAARQVRQDIGRDNVFFVHVSLVPYLAPSGELKTKPTQHSVAALRSIGIQPDAIVCRSDREVPDGIRRKIGSSCDVDFEAVISCMDAPSIYDIPKVLHSGGLDAYLIRKLDLPFRDVDWTVWGDLLDRVHNPKHELPDSYKSINEAFVHAGAQNDCKVNVKWISSEEINPETCEDILAGLDGILVAPGFGERGMEGKIEAIRYVREQKIPFFGVCLGMQCAVIEFARNVLKLHGASSTELDPNTRHPVIDLMEEQKKVTEKGGTMRLGAYACRLKKGSKAYNIYGESIIHERHRHRYEFNNKYLNDIEEAGLKAVGVNPESGLVEVVELKDHPWFIGVQFHPELKSTVLNPHPLFVKFVGLVAAAKNRKVRIAVVGKYTDLPDAYKSVVEALIHGGITNDAKVEIEWIASDRFSDIESAGELLGGVDGLLIPGGFGERGIEGMVQAVRWAREHELPFFGICLGLQVAIIEFARNVCELPDSNSTEFEPECETPVIALMTSQHDVENLGGTMRLGAYPARLRKGSRVAQVYGSTEISERHRHRWEVNNAYRDVLAEFGMRLSGQSPDGGLVEIIELPNHPWFIGCQFHPELKSRPTRPHPLFERFLGYVGRLQARAADPSRGAAG"
  },
  {
    "reference": "MTGIVLAAGQGKRMKSRLPKVIHRILDRPMINWVAASLRRAGVDRIVVVTGFEAERVEAVLDEDVLSVRQIPQLGTGHAVMTAAEYLDDEEVVVVAGDEPLVRVSTLERLILTRRNGDYDVVFLTMKPADPTGYGRVVKTGDKIRIVEHRDADQETRKIDEVNTAIYAFKGSFLRDAVKRLSNDNDQKEYYLTDTVAMAGRADTIVVEDTDEVLGVNDRIQLAEITRIAKKRINEEHMRNGVTMVDPETCYIGPDVEIGIDSVIEPMVFITGKTRIGDNCLIGPMTRIENAIVEDDVEIIRSEVDSAEIRSGARVGPFSRLRPGAVVMNNAHVGNYVELKKTVLGKGSKAQHLTYLGDTNVGENVNIGAGTITCNYDGKNKHKTEICDSAFIGSNTSLVAPVKIGERSVTAAGSAITEDVPPDSLAFGRARQVVKEGRYPKNRGQEEKN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.2. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+), acetyl-CoA, UDP-N-acetyl-alpha-D-glucosamine binding.3. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.4. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate. The designed protein sequence is ",
    "response#1": "VLAGGLGTRMKSEEPKALSALLENPLIFYTVEALINTRRAFNIYRGYKHRVIIGKIGIVIGHKGESVKNYILKEKRFKVKGVLFDFAEQEKYLGTGDAALKALDMFTPADDESSLLILPCDMPLIEAKTFEDLIKFHLDNENDLTVLSVKAENPYSYGRILKDKNGHVKEIVEENELANYPKNIGEIKEINSGVYVVKLNHLKRLIKKIKPDNLKKEYYFTDIVNIFFKNGLKTMCSHIAEEGIVEGVHLIDPQRFDLRGELLVGQDVCIDINVIIEGHCEFGNNVQIGAGCIIKNTKIAAGTVVKPYSIFEDANVGENTAIGPFARLRPGTHLANEVHIGNFVEVKNSNIGLGSKANHFTYLGDAEVGAGSNIGAGTITCNYDGANKFKTIIGDHAFIGSNSSLVAPVVIANGATVGAGSTITRDVAENSLAVERSKQKNIIGWRNKK",
    "response#2": "VVILAAGQGTRMKSRRSKVLHEIAGRPMVGFPVALARELSASEPVVVVGRDAEDVQAALAGQAKFVLQAEQNGTGHAVLCAQDALADARDAILILYGDTPLLRLESLRRMAEMLAADDADLVMLTSPEDPTGYGRIVREDGQVVAIVEEKDATEAQRQITEVNSGVYVFRAGALRAVVEALTTDNAQGEKYLTDVLEILRRDGQRVSAHKAADHWQTLGVNNRLQLAEAERVLRERVNQRWMLAGVTVIDPATTWVDVDVTLEADAVLLPNTQLHGTTHIAAGAEVGPNCTLRDTRVGAGAHVTNATCDGAVIGPDAAVGPYTYLRPGTVLAANAKAGGFVEMKNAQVGEGSKVPHLSYVGDATIGAGANIGAATIFVNYDGVAKHHTVVGDHVRVGSDSMLVAPVTIGDGAYTAAGSVITEDVPPGAMAVARGRQRNIEGWVARKRAGTPSAAAAERARSREPEQD",
    "response#3": "MTVVILAAGQGTRMKSRRSKVLHEIAGRPMVGFPVALARELSASEPVVVVGRDAEDVQAALAGQAKFVLQAEQNGTGHAVLCAQDALADARDAILILYGDTPLLRLESLRRMAEMLAADDADLVMLTSPEGGLGRIIRDATGNVLAIVEEKDATDAQRAIDEIYSGIMAVPTALLKDWLARLDNANAQGEYYLTDLIGHAVSEGVPVVAHHLEDALQTEGVNNRVQLAELERAHQLRQAEALMLAGVTVIDPATTWVDVDVTLEADAVLLPNTQLHGTTHIAAGAEVGPNCTLRDTRVGAGAHVTNATCDGAVIGPDAAVGPYTYLRPGTVLAANAKAGGFVEMKNAQVGEGSKVPHLSYVGDATIGAGANIGAATIFVNYDGVAKHHTVVGDHVRVGSDSMLVAPVTIGDGAYTAAGSVITEDVPPGAMAVARGRQRNIAGWKRPAKKK"
  },
  {
    "reference": "MKTIEVDEELYRFIASQTQHIGESASDILRRLLMQSSPSDLAVSAPVEVQKKGIVVSKDAGKEASVDRVKAVRTLLISDEFSALDKAIDRFLTVLSELYKIDSKAFSEATEVKGRTRVYFADNQETLIASGKTTKPREIVGTPFWVITNTNTNRKRHMVELLMERMGFQHDLTEKVCAAI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should be able to enhance the efficiency of regulation of DNA-templated transcription, negative regulation of DNA-templated DNA replication initiation.2. The protein must exhibit the following characteristics: Repression is relieved when the region becomes fully methylated.; Binds to pairs of hemimethylated GATC sequences in the oriC region, thus preventing assembly of replication proteins and re- initiation at newly replicated origins. The designed protein sequence is ",
    "response#1": "MFNPLYIHAGVGLGKTHLLQAVTWAGNAGGERKVLYLTAEKFMYGFVAALKSQTALAFKEALRGIDVLVIDDLQFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVV",
    "response#2": "MFNPLYIHAGVGLGKTHLLQAVTWAGNAGGERKVLYLTAEKFMYGFVAALKSQTALAFKEALRGIDVLVIDDLQFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVS",
    "response#3": "MFNPLYIHAGVGLGKTHLLQAVTWAGNAGGERKVLYLTAEKFMYGFVAALKSQTALAFKEALRGIDVLVIDDLQFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSDLESLDDRVRSRLAGGLVG"
  },
  {
    "reference": "MSAVMIAVVLLGLLALVFGAILGFAAVKFRVEGDPLVDQVESLLPQTQCGQCGYPGCRPYAEAIAGGDQINKCPPGGTATMEKIAELMGVEPQPLSATTEQQVKKVAYIREDECIGCTKCIQACPVDAIVGAGKLMHTVITQDCTGCDLCVEPCPVDCIDMLPVTQDIKTWNWKLSAIPVMQLEEDKQC",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must have high specificity towards 4 iron, 4 sulfur cluster binding.2. The protein must exhibit the following characteristics: Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane..3. The protein must bind to [4Fe-4S] cluster in order to perform its enzymatic function.4. The [4Fe-4S] cluster binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "KVVGIGSPRASLESNFALRELVGEGNFYSGINEGELDRLRLILQVMQEGPLPVPSIRDIEDHDAVFVLGEDLTQTAARIALALRQSVKGKAVEMAADMKVQPWLDAAVKNIAQHAQNPLFIASVSATRLDDVAEETVHAAPDDLARLGFAVAHAIDPSAPSVADLDPQAQAFAQRITDALLAAKRPLVVSGNSLGNKALIEAAANIAKALKQREKNGSISLVVGEANSLGLALFGGDSVEAALERLTSGQADAVVVLENDLYRRTDAARVDAALAAAKVVIVADHQQTATTAKAHLVLPAASFAEGDGTLVSQEGRAQRFFQVFDPTRASVVEWLMTNHPHDCPVCEEGGHCHLQDMTVMTGHNERC",
    "response#2": "KVVGIGSPRASLESNFALRELVGEGNFYSGINEGELDRLRLILQVMQEGPLPVPSIRDIEDHDAVFVLGEDLTQTAARIALALRQSVKGKAVEMAADMKVQPWLDAAVKNIAQHAQNPLFIASVSATRLDDVAEETVHAAPDDLARLGFAVAHAIDPSAPSVADLDPQAQAFAQRITDALLAAKRPLVVSGNSLGNKALIEAAANIAKALKQREKNGSISLVVGEANSLGLALFGGDSVEAALERLTSGQADAVVVLENDLYRRTDAARVDAALAAAKVVIVADHQQTATTAKAHLVLPAASFAEGDGTLVSQEGRAQRFFQVFDPTRASVVEWLMTNHPHDCPVCEEGGHCHLQDMTVMTGHNERRC",
    "response#3": "KVVGIGSPRASLESNFALRELVGEGNFYSGINEGELDRLRLILQVMQEGPLPVPSIRDIEDHDAVFVLGEDLTQTAARIALALRQSVKGKAVEMAADMKVQPWLDAAVKNIAQHAQNPLFIASVSATRLDDVAEETVHAAPDDLARLGFAVAHAIDPSAPSVADLDPQAQAFAQRITDALLAAKRPLVVSGNSLGNKALIEAAANIAKALKQREKNGSISLVVGEANSLGLALFGGDSVEAALERLTSGQADAVVVLENDLYRRTDAARVDAALAAAKVVIVADHQQTATTAKAHLVLPAASFAEGDGTLVSQEGRAQRFFQVFDPTAP"
  },
  {
    "reference": "MSAKTAVLLMAYGTPNRIDEVEQYYINVRGGRMPTPEQVENLSARYRAVGGHTPLTTLTKSVTDQLQAQLDAEFPDQYQVYFGMKYWHPLIPDVVKQIHADGISKVIGLALAPHYSKISIGGYQKQVDRANEEFNTNIELTMINSWQEQPKFRNLIANRISEALAQFPADVRDQVTVLFSAHSLPQRVLAWGDPYPDELLGSAKGIAEMLELPDWRFTYQSQGETGEPWLGPDVLDTLAELAAEGKKYVLQVPFGFVCDHLEILYDIDIEGKHKANELGLQLERIRLLNDDPAFVDLLKTVVTGQ",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Catalyzes the ferrous insertion into protoporphyrin IX..2. The protein should have a high affinity for Fe cation.3. The protein design must be able to perform ferrochelatase activity in order to contribute to heme biosynthetic process. The designed protein sequence is ",
    "response#1": "MVELISEQISLCESPSKAHIFFTAHGVPKSYVEEAGDPYKQQIEDCSLLIINELEKCLGYSNPHTLSYQSRVGPVEWLKPYTEEVLADLGRSNVNDLVVVPISFVGEHIETLQEIDIEYKEIAEKAGIKNFRRVKALF",
    "response#2": "EAHANLITKHYKLASEVGKPRVLFSAHSLPLSVIKKGDPYALQVEETVKLIVKKLHIKDLDWSICYQSKIGPVKWLEPSTESELLRAKADGVPVVLLPISFVSEHSETLVELDMEYKTIIKDGYYFRIPTPNYS",
    "response#3": "MVELISEQISLCESPSKAHIFFTAHGVPKSYVEEAGDPYKQQIEDCSLLIINELEKCLGYSNPHTLSYQSRVGPVEWLKPYTEEVLADLGRSNVNDLVVVPISFVGEHIETLQEIDIEYKEIAEKAGIKNFRRVKALE"
  },
  {
    "reference": "MVNIGRNLALWVIIALLVFALFNLFQGSTPRGTQQPLAYSDFLSQVETGDVREVTIKGRQITGVYRDGRSLTTYAPEDPNLISDLQKHGVRIAATPADDGSPTIWSVLISWFPMLLLIGVWIFFMRQMQAGGGKAMGFGKSRARLLTEKTGRVTFEDVAGIEEAKQELEEVVDFLKDPQKFQRLXGKIPKGCLLVGPPGTGKTLLARAIAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLNQLLVEMDGFESNDGVILIAATNRPDVLDPALLRPGRFDRQVVVPNPDILGREQILKVHMRKVQVGADVDPRVIARGTPGFSGADLANLVNEAALLAARAGKRLVTMFDFEAAKDKVLMGVERRSMVMSEEEKRLTAYHEAGHALVGLHVPLHDPLHKVTIIPRGRALGVTMSLPERDRHSYSKLELESKLAVLFGGRGAEEIVFGADNVTTGAGNDIRQATDMARRMVTEFGFSEKLGPLRYTDNQEEIFLGHAVTQQKHVSDATARLIDEEVRRLIDDAEEKARSILNSHRSDLETLASALLEYETLSXDEVEALLRGEPVVRVDPRDEPRETGRRASVPSSGKKVGKEPHGGLEPQPN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a high specificity for Zn(2+), ATP binding, with minimal off-target effects.2. The ideal protein would be one that plays a critical role in proteolysis, protein catabolic process through ATP binding, ATP-dependent peptidase activity within plasma membrane.3. The designed protein must have a active site that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "ILMVGPPGTGKTLLARAVAGEAKVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKHSPCIVFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFEENLGVIVIAATNRPDVLDAALLRPGRFDRQVMVA",
    "response#2": "IPKGVLLVGPPGTGKTLLGKAVAGEAGVPFFSLSGSDFVEMFVGVGAARVRDMFQQAEQRAPCIVFIDELDALGKTRGSGNMGGHDEREQTLNALLVEMDGFGTNSGIIIMGATNRPETLDPALMRPGRFDRHVLVDRPGMADDELLSVIEAA",
    "response#3": "IPTGVLLVGPPGTGKTLLAKAIAGEAKVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKSAPCIIFIDEIDAVGRQRGAGVGGGHDEREQTLNQLLVEMDGFEGNEGVIVIAATNRPDVLDAALLRPGRFDRQVVVGLPV"
  },
  {
    "reference": "MVRKYFGTDGIRGRANGVITPELALKVGQAAGLVFQRGDHRHRVVIGKDTRLSGYMIETALVAGFTSVGMDVLLLGPVPTPAVAMLTRSMRADLGVMISASHNPFEDNGIKLFGPDGFKLNDAIEHEIEGLIDADMHKRLSGSNDLGRAKRIESVHARYIEFAKRTLPRQVTLDGLRVVVDCANGAAYRVAPETLWELGAEVIAIGTEPDGFNINRDVGSTAPAALIDMVRERRADIGIALDGDADRVLIVDEKGQVVDGDQLMAVVARSWKEDERLTQPGVVATIMSNLGLERFLGGLGLSLARTAVGDRYVLEHMRAHGYNLGGEQSGHIIMSDYTTTGDGLVAALQLLSVVQRQNRPVSEVCHCFDPLPQILKNVRYRSGEPLREDSVVSAIEHARERLGNAGRLVIRPSGTEPVIRVMAEGDDRGLVNAVVDEVVDAVTRAAA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must have high specificity towards magnesium ion binding, phosphoglucosamine mutase activity. The designed protein sequence is ",
    "response#1": "DGDEILFILAQAAHSKGEMTGGVVGTLMSNLGLELALKQMDIPFVRAKVGDRYVVEQLKRTGWQLGGEGSGHILSLQHASTGDGIVASLQVLKAVLESQKSLSEIKAGC",
    "response#2": "NGDELLYLLAADRLSRGENVPGVVGTLMTNMAVELALKADGVELVRAKVGDRYVLEELARRRWLLGGESSGHLLALDRHTTGDGLISALQVLQACVRGGRSLARTLEHH",
    "response#3": "DGDEILFILAQAAHSKGEMTGGVVGTLMSNLGLELALKQMDIPFVRAKVGDRYVVEQLKRTGWQLGGEGSGHILSLQHASTGDGIVASLQVLKAVLESQKSLSEIKAGE"
  },
  {
    "reference": "MKLSELTKQIRQAECTGDSDPDVKGLCYDSRKAGSGDLFFALKGEKYDGLDYVESAINSGSVAVVAETENPNSDHAVPWVKVPNGREAMSVMSDIFNNSPSXELTIIGVTGTNGKTTTSYMIHXLLDLGHRRSGLIGTIKYDLKSDNEIEATRTTPEAIDLQNYLSQMISNGCCSAVMEVSSHALSQSRTSHVXYDVGVFTNLSHDHLDYHGNMXRYFNAKKXLFXQLSNNEVKNKAVAVINXDDDWGRKLVHYLSNKKVKTVTYGLANGADYRAVDLRLTKTSIEFAIEVGSRKFKSSIPLVGRFNIYNALAALASCAAVGLNVRETVNNLKQLPQIPGRLESVSSGRAFEVFVDYAHTPDALEKTLLSIKELDPRRIILVFGCGGERDLEKRAKMGDVASRFSNYSIITSDNPRSEDPMEIIKGIESGFGGSHYEILEDRREAIAKGVSYLAPGDVLLIAGKGHEDYQMVGKDRLEFDDRLVARQMLNDRDHVRHEMVRESRRLREEQEAERKNKFYDGEEVGRN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein have Mur ligase central domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "VVGTNGKTTITYILESIFAAAGFRCGVLGTVNYRYPGIRMAAPNTTPESMDLQRILRDMAESGVTHLVAEVSSHAIDLRRVDDCRFDLGIFTNLTQDHLDYHKTMENYYQAKKRFFSEVLPGGRKLRRHSMVVNVDDPWGKRLVRETGAGNRPLTFGIEENADVRPSCFDLSLDGIKAVIEVGPASFEMASPLIGKFNLSNVLAAVSAI",
    "response#2": "VTGTNGKTTVTTAIYLALDNCGIKSGLIGTIEYRIGESSIPAKFTTPEAYDFFSLLRRMRDSGCAFASIEASSQALVQRRVYGEVFDIAVFTNLSVDHLDYHGDMESYYSAKKLLFSSAKKAVINIDDKYGKRLSRELDGDVVTFSVCEEADYNAEGITHSVEGTKFVLKGKYFKESVSFPISGDFSVYNAAMAAIS",
    "response#3": "VTGTSGKTTTSYLLEAGLLHAGYSVGLIGTTGTRINREPVPTSLTTPEAPTLQELFARMLSEGVTHVVMEVSSHALELGRVRGTRFDVGGFSNLSQDHLDFHPTMEDYFEAKAALFDGPQAAERAVICVDDDWGKRMAKRAAALPLTRVATQGEADITARQLSTEATGAQQVDLTIAASGTSYEFRLPLPGEFNIANAALATGVTGTNGKTTITQLIAQWLELLGHKAAVMGTTGNGFLNALEPAANTTGNALQIQATLRDLAERGAQFTALETSSHGLVQGRVKKLNFVAGVFSNLSRDHLDYHGTMEAYAQAKFSLFSEHTCQNAIINVDDAVGAQWVKQLPNAIGVSLLAKPETEQAIWASQVAYAESGITLNFESSWGKGELHAPLIGEFNACNLLLALAIL"
  },
  {
    "reference": "MGKDTLSEIVTSIRNADMAKKETVRITFTNIAQNIVTILLREGFIKNAREHRESKKSFLVLTLRHRRNRKGPSRTLFNLKRVSRPGLRIYSNYQKIPRILGGMGVAILSTSKGIMTDREARLKRIGGEILLYIW",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that can perform rRNA binding, structural constituent of ribosome in chloroplast, ribonucleoprotein complex, ribosome to support translation.2. For general function, the protein need meet that One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit. The designed protein sequence is ",
    "response#1": "LESRLDNLVYRLGLARTRRQARQLGV",
    "response#2": "MLPSRLDNLVYRLGLARTRRQARQLVTHGHIIVDGSRVNIPSYRVKPGQTIAVREKSRNLQVIKEALG",
    "response#3": "MISAAFRFH"
  },
  {
    "reference": "MEKIKVCLVDDNKELVSMLESYVAAQDDMEVIGTAYNGQECLNLLTDKQPDVLVLDIIMPHLDGLAVLEKMRHIERLKQPSVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLTSHIRQVSGKANAMIKRPLPSFRSATTVDGKPKNLDASITSIIHEIGVPAHIKGYMYLREAISMVYNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVSMSKAKPTNSEFIAMVADKLRLEHKAS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be able to bind Ca(2+) ligand in a variety of conditions2. The ideal protein would be one that plays a critical role in regulation of sporulation resulting in formation of a cellular spore, phosphorelay signal transduction system through DNA-binding transcription factor activity, DNA binding within cytoplasm.3. The presence of Ca(2+) is necessary for the protein to function in the desired environment.4. The protein must exhibit the following characteristics: It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress; May play the central regulatory role in sporulation; Repressor of abrB, activator of the spoIIa operon. The designed protein sequence is ",
    "response#1": "RSGLARDLHDSVLQDLISLKRQCELFLGDFKKDDNPCREEVQDKLVQMNEQMSDVISMTRETCHELRPQL",
    "response#2": "IKVCLVDDNRELIGLLEDYISEQEDMEVIGVAYNGQECLSILKDKNPDVLVLDIIMPHLDGLAVLGQLKEMKKENYPNVIMLTAFGQEDVTKKAVDLGAAYFILKPFDMENLVNHR",
    "response#3": "LYRIIQEFLSNAVKHSQATDVLIMLISIQNKIVLHYEDDGVGFDQEKNTEHSMSMGLSGIKERVRALDGRLRIETSEGKGFKADIEIE"
  },
  {
    "reference": "MTAQSTSLTQSLAQLDPEVAAAVDAELARQRDTLEMIASENFAPRAVLEAQGTVLTNKYAEGYPGRRYYGGCEHVDVIEQLAIDRAKALFGAEHANVQPHSGAQANTAVYFALLQPGDTILGLDLAHGGHLTHGMRINYSGKILNAVAYHVRESDGLIDYDEVEALAKEHQPKLIIAGWSAYPRQLDFARFREIADQTGALLMVDMAHFAGLVAAGLHPNPVPYADVVTTTTHKTLGGPRGGLILAKEELGKKINSAVFPGMQGGPLQHVIAAKAVALKVAASEEFAERQRRTLSGAKILAERLTQPDAAEAGIRVLTGGTDVHLVLVDLVNSELNGKEAEDRLHEIGITVNRNAVPNDPRPPMVTSGLRIGTPALATRGFGDADFAEVADIIAEALKPGFDAATLRSRVQALAAKHPLYPGL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a fast turnover rate for (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O =   (6S)-5,6,7,8-tetrahydrofolate + L-serine.2. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in glycine biosynthetic process from serine.3. The protein should be optimized for activity in glycine from L- serine: step 1/1 pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The protein must exhibit the following characteristics: Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.; This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. The designed protein sequence is ",
    "response#1": "LSAVDPEIAAIMGREQARQRRGIELIASENFASAAVRAAAGSVMTNKYAEGYPGKRYYNGCVHVDEAENLAIERACKLFGAEAANVQAHSGSQANMAAYLAVLNPGDTVLAMRLDHGGHLTHGHSVNFSGRTYNFVGYGVSPETEMLDYDDIARLARENKPRLILAGASAYPRSIDFARLRAIADEVGALLMADMAHIAGLVAAGIHPSPVPYCDIVTSTTHKTLRGPRGGLILAKPDLMKKINSQVFPGIQGGPLMHVIAAKAVCFHEALQPSFAAYQRQVVKNAACLAGELMNRGFRIVSGGTDNHLMLVDLRPKGVTGKASATALDKADITVNMNLIPFDPEKPFITSGIRIGTPAVTTRGMKEPEMREIARLIAEALATPADDALAQTDPELAKAIELEHQRQQRNIELIASENFVSPAVLEAQGSILTNKYAEGYPGKRYYGGCEFVDIAESLAISRAKKLFGADHANVQPHSGAQANFAVYFALLQPGDKILGMNLAHGGHLTHGSPVNVSGKYFNVVAYGVEEDTGCINYEKLREIALQEKPKMIVAGASAYARAIDFKKIGEIAKEIDAYFFVDMAHIAGLVAAGLHQSPVPYADVVTTTTHKTLRGPRGGMILCKEEYAQLIDKAIFPGSQGGPLMHVIAAKAAAFGEALKPEFKAYQQQIINNAQALAKGLLERGFNLVSGGTDNHLILVDLRGTGITGKQAETLLDEVHITCNKNAIPFDPEKPFVTSGIRLGTPAVTTRGFKEKDMDKVAEIIADALRTPAGDAGVAARVRELTASFPLYPGLA",
    "response#2": "LADTDPEIFGAIGKELGRQRHEIELIASENIVSRAVLEAQGSIMTNKYAEGYPGKRYYGGCQYVDIAEELAIERAKKLFGVNFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHLTHGSPVNMSGKWFNVVSYGVREGDNLLDMEAVQRKAEETKPKLIIAGGTAYSRIWDWKRFREIADSVGAYLMVDMAHIAGLVAGGQHPSPFPHCHVATTTTHKSLRGPRGGMILTNDEDLAKKFNSAVFPGLQGGPLMHVIAAKAVALGEALQPEFQDYAAQIVKNAKALSETLISGGVDVVSGGTDNHLMLVDLRKKNATGKRAEAALGRAYVTCNKNGIPFDPEKPFVTSGVRLGTPAGTTRGFKEAEFREIGNLIVEALNAIRDDAREADPAVADALEGERGRQNDTLAMIASENHVSEAVMEAQSSELTNKYAEGYPGERYYGGCEYADDVEELAIDRAKELWGADHVNVQPHSGSQANMGVYLGVLEPGDKILSLDLTHGGHLSHGHPANFAGQVYEVEQYKVDEETGYVDYEGLHDHAEEFEPDIIVSGYSAYPREVDFERIQEAADAVDAYHLADIAHITGLVAAGVHESPVGVADFVTGSTHKTIRAGRGGIIMCDEEYADDIDAAVFPGSQGGPLMHNVAGKAVGFGEALAPEFEQYAQQTVDNAIALGEQFKEHGLSLVSGGTDNHLVLIDLRPSHPDTTGKEVEEALEEAGIVLNANTVPGETRSAFNPSGIRAGTPALTTRGFDEDACREVADLIADAVTAVAART",
    "response#3": "QDPEVGKAIILEIERQTGKLELIASENFVSAAVRQAQGSVLTHKYAEGYPGKRYYGGCEFVDIAENIAIERARTIFGCEYANVQPHSGSQANMGVYFACLKPGDTILGMNLSHGGHLTHGSPVNFSGRLFNVVFYGVEKETGRIDYEQVAALAREHKPSLIVAGASAYPRTIDFARFRAIADEVGAKLMVDMAHIAGLVAAGYHPSPVQHAHYTTTTTHKTLRGPRGGMILSTEDNGKTLNSQIFPGIQGGPLMHVIAAKAVAFGEALRPAFKEYQKQVVDNAAALAGVLTAAGFDLVSGGTDNHLMLVDLTSKDVTGKDAEIALDKAGITVNKNTVPFETRSPFVTSGVRLGTPALTTRGMKAAEMEKVGGWLGDVLTPADAQRVRDLHELDPDVAAAVDAELDRQQSTLEMIASENFAPVAVMEAQGSVLTNKYAEGYPGRRYYGGCEHVDVVEQIAIDRVKALFGAEHANVQPHSGAQANAAAMFALLKPGDTIMGLNLAHGGHLTHGMKINFSGKLYNVVPYHVGDDGQVDMAEVERLAKETKPKLIVAGWSAYPRQLDFAAFRKVADEVGAYLMVDMAHFAGLVAAGLHPNPVPHAHVVTTTTHKTLGGPRGGVILSTAELAKKINSAVFPGQQGGPLEHVVAAKAVAFKVAASEDFKERQGRTLEGARILAERLVRDDAKAAGVSVLTGGTDVHLVLVDLRDSELDGQQAEDRLHEVGITVNRNAVPNDPRPPMVTSGLRIGTPALATRGFTAEDFAEVADVIAEALRTPATADRLERLRADVARLCAAFPLYPGIELP"
  },
  {
    "reference": "MGSMMAFAFSEIGLDVSIWDVKYDNVQQLLESAKNTNYKGKIEGFKDVSKFTQSLEGKAERKIFLFSITHGDPADSVLDMIKKDLKKGDIILDGGNENYRRTEARQKICEKIGVSWIGLGVSGGYQSARRGPSLSPGGDKEALDLVMPLLELYAGKDAKSGQPCVTRIGPKGSGHFVKMVHNGIEGGMLSTLAEAWSLLHYGRGMGYEEIADLFESWNKEGVLRNNYLLEIGAELLRVKKTPQGDGNGEGVGDGGFVLDDVLDKVVQDDDNTEGTPYWSVMESASRHISAPTLATAHFMRIASGNRIERLEVAKQLRIPSPSPIRGMKDIEAFKEHLHSAVYSSFLASFCQGLELIARASEDEGWDIDLGKCLQIWRSGCIIRSEGIADILQPAVSGNKGIKNMKYIDTVAQELHRTYPSLKEIVMAATDSDHYIPAISATLEYLKYEGGTNLPTKFMEAQMDFFGAHGYNLPGVPGEDPGPVSKGPHHYEWRPAK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have NADP binding, phosphogluconate dehydrogenase (decarboxylating) activity to facilitate D-gluconate metabolic process.2. The protein must exhibit the following characteristics: Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH..3. The protein with the reaction 6-phospho-D-gluconate + NADP(+) = CO2 + D-ribulose 5-phosphate   + NADPH, the Proton acceptor, Proton donor must be optimized to ensure efficient catalysis.4. We require a protein that can specifically bind to the metabolites produced in the Carbohydrate degradation, pentose phosphate pathway, D- ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3 pathway.5. The designed protein should be able to compete with endogenous proteins or inhibitors for NADP(+), substrate binding. The designed protein sequence is ",
    "response#1": "MTIRFGNKILSSTWNRENIASVLITFKEPFGTQGRGGYFDEFGIIRDVMQNHLLQILSLVAMEKPVSCHPDDIRDEKVKVLKSIEALTLDDMVLGQYLGNPQGTNDDARTGYVEDPTVSNDSNTPTYALGVLKINNERWQGVPFILRCGKALNERKAEVRIQYQDVLGDIFEGNTKRNELVIRVQPGEALYFKMMTKSPGITFDIEETELDLTYEHRYKDSYLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPILHQIEKEHIRPITYQYGSRGPKEADRKCEENNFKLGHFVKMVHNGIEYADMQLIAEAYTFLREKLLLPIDEIADIFDTWNQGELKSYLIEITAEILRKKDERTGAPLIDVILDKTGQKGTGKWTSLQAIDNGIPSSIITESLFARYLSSLKDERTAAENVLAGPETEERPLDQNVWIDRVRQALYMGKVCAYAQGFAQYKMTSDLNGWHLPLKDIALIFRGGCIIRAQFLNLISEVYDKQPDLSNLLVAPDFAEKLKEYQSGLRKVVCEGISSGISFPCLSTALSYYDGYRTGRSNANLLQAQANYFGAHTYERTDMEGVFHTDWYNGTKLPPELAGHFVKMVHNGIEYADMQLIAEAYTFLREKLLLPIDEIADIFDTWNQGELKSYLIEITAEILRKKDERTGAPLIDVILDKTGQKGTGKWTSLQAIDNGIPSSIITESLFARYLSSLKDERTAAENVLAGPETEERPLDQNVWIDRVRQALYMGKVCAYAQGFAQYKMTSDLNGWHLPLKDIALIFRGGCIIRAQFLNLISEVYDKQPDLSNLLVAPDFAEKLKEYQSGLRKVVCEGISSGISFPCLSTALSYYDGYRTGRSNANLLQAQANYFGAHTYERTDMEGVFHTD",
    "response#2": "MTIRFGNKILSSTWNRENIASVLITFKEPFGTQGRGGYFDEFGIIRDVMQNHLLQILSLVAMEKPVSCHPDDIRDEKVKVLKSIEALTLDDMVLGQYLGNPQGTNDDARTGYVEDPTVSNDSNTPTYALGVLKINNERWQGVPFILRCGKALNERKAEVRIQYQDVLGDIFEGNTKRNELVIRVQPGEALYFKMMTKSPGITFDIEETELDLTYEHRYKDSYLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPILHQIEKEHIRPITYQYGSRGPKEADRKCEENNFKREVMSNRYVKSVEIVSKEVLGGEGRHYFDKNGIIRDMVLSHMGELLGVVASDVTKPSRIMEALARMEVFKACTVDTERCIYGQYDDYIKEIHKDSSTETFCMVPISIGTPRWSKVPFIIVAGKGMNEKRTEIILEFRRDVFAKCIELFSMPRQSSCRIVHTNEIETVRLVFNIYPECEVFLEVLVGGEPVRYVLHDKKEIDGLMHDSYGGYHDYEIIFDSLVRGKDFSSVSSQEAELLWKVFNPILSISKEDMLFYYSKGIDMPKEAEEMIRREVMSNRYVKSVEIVSKEVLGGEGRHYFDKNGIIRDMVLSHMGELLGVVASDVTKPSRIMEALARMEVFKACTVDTERCIYGQYDDYIKEIHKDSSTETFCMVPISIGTPRWSKVPFIIVAGKGMNEKRTEIILEFRRDVFAKCIELFSMPRQSSCRIVHTNEIETVRLVFNIYPECEVFLEVLVGGEPVRYVLHDKKEIDGLMHDSYGGYHDYEIIFDSLVRGKDFSSVSSQEAELLWKVFNPILSISKEDMLFYYSKGIDMPKEAEEMIR",
    "response#3": "MTIRFGNKILSSTWNRENIASVLITFKEPFGTQGRGGYFDEFGIIRDVMQNHLLQILSLVAMEKPVSCHPDDIRDEKVKVLKSIEALTLDDMVLGQYLGNPQGTNDDARTGYVEDPTVSNDSNTPTYALGVLKINNERWQGVPFILRCGKALNERKAEVRIQYQDVLGDIFEGNTKRNELVIRVQPGEALYFKMMTKSPGITFDIEETELDLTYEHRYKDSYLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPILHQIEKEHIRPITYQYGSRGPKEADRKCEENNFKLVFRFANFIFEEIWNNKFVDHVQITMAEDIGVEHRAGYFENVGLLRDIFQNHMLQILALIAMEPPSSFNGENFRNERVKLLRSIRPFPVEELESWIVRGQYGRGVVNGKEVPAYREEPGVAKDSNVETFVAMKLFIDNWRWSGVPFYLRSGKRLPKKITEVAVVFKKIPHSIFAGVPSDELEPNTIVFTLQPNEGISLEFQVKRPCPGMFPQLLSMDFRYEDYFGVKLPDAYERLLLDVILGDPTLFMRRDDLEVSWELLDPVLKAWENDPVRFSPYVYPAGTWGPREADLLIERDGRKWVLRFSNLIFEPLWSRQYIRNVQFIFSEDFGTEGRGGYFDNYGIIRDIMQNHLLQILALFAMETPVSLDAEDIRNEKVKVLRSMRPIRVEDVVIGQYKSHTKGGVTYPAYTDDKTVPKGSLTPTFAAAALFIDNARWDGVPFLMKAGKALHTRSAEIRVQFRHVPGNLYNRNTGSDLDQATNELVIRVQPDEAIYLKINNKVPGLGMRLDRSNLNLLYSARYSKEIPDAYERLLLDAIEGERRLFIRSDELDAAWSLFTPLLKEIEEKKRIPEYYPYGSRGPVGAHYLAAKHKVQ"
  },
  {
    "reference": "MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species ; Acts as a gatekeeper of the core 20S proteasome known to degrade proteins with unstructured regions; Alternatively, can activate quinones and their derivatives by generating redox reactive hydroquinones with DNA cross-linking antitumor potential; Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms.2. The FAD binding site should be stable and able to withstand changes in environmental conditions.3. The protein have enhanced H(+) + menadione + NADH = menadiol + NAD(+).4. The ideal protein would be one that plays a critical role in response to alkaloid, cellular response to oxidative stress through NADH dehydrogenase (quinone) activity, NAD(P)H dehydrogenase (quinone) activity within nucleus, neuronal cell body, synapse. The designed protein sequence is ",
    "response#1": "DIGSMSTVFLAFTDRQKIYDLIESITGARMHPAWFRIGGVAKDLPKNWNSLLKKFLIWMPKRLDYYVNIAIKNKILISRSKGIAAYGKKEALSWGITGAGLRATGVNFDVRKNRPYSGYQNFDFEVPIGNNISDAYTRVLLKIEEIRQSLKILNQCLLHMPLGSYKSDHPLSTPQKKNKSLFHIERMITHFLQMSWGPIIPSNESFQMIEATKGINSYYLISDGLTTSYRTRIRTPSFAHLQQIPSVIRGYVISDLIVYLGSIDFVMSDVDEVLLRDRIILGIKDKNIQHELRDKKKLTLNEAIDEVRDYKLQREQIQFKNKQNTSKIDAVTVNQVKKPKTKPKCKYCGSFHKPNECPAFGKTCHKCHKRNHFARMCKSTKVNMVQTVNDSESESDHLFGLQPINKVNLKKEKPIVLTVNQKLVNFKIDTGAQCNVISQETYINMKKKPKLHPTNIILKSYTGNKILPLGKIELQCKYKNQSKTLPLIVIKGKGPNLLGRKSSESLGLIQRIHTVNTVQSSDINTIFPQVFPGLGKLKDNYKITLKENAVPVVQPARRLPFSLREKVEKELDRLEKLDIISPVDQATEWCAGMVVVQKKDKKIRICVDPMPLNKALKREHYQLPTLEEILPKLANAKIFSVLDAKDGFWQIKLDEASSYLTTFNSPLGRYRWLRLPFGISPAPEIYQHRLQQSLEGINGVKCVADD",
    "response#2": "DIGAMTVFLYAFREREQILQFFEKICGARLTVSYPRIGGVRVDIKEHVLDEIYKFMDLMLIRVDEYETLLTENRIWIARTRGVGVIAPEDAVLLGLTGPALRGSGVYYDIRKQIPYDAYSEIDFEVPLGEKGDTYDRYLCRIREMRQSVLIVKQCIEKMPEGKILSDKSPDIDLPHQAKRKIEPGDSLWNGFIAFSEEKQEIMPKGEIYSAIEAPKGELGFYIVSDGSGRPYRMRVRAPSFIHISAIPKLCEGHLLADVIAIIGTLDIVMGEADWIDSTILIQVIGFLKKNNTCSYEILFDLFGIDERTRKNVKSFPNADFTVSYYFFSISNNSDLILKVALLKEHLQLPTISSIFLNANWYEREIWDMFGIVFNNHPYLTRILMPKNWVGHPLRKDYSSHDLGATTPFLYCWRDREKLYSLFERITGGRMLYNYLRIGGVRNDLPEGILGTPQDGEDKADKTIWGFINYFDSYVYPEWKALVTGNRIFQYRTKNIGVLTAEQAIAYSCSGAVLRGSGVKWDLRKNLPYAIYDRFEFDIPVGQNGDSFDRWWVRQEEMYQSSRIVKQCLEWLAENPGPVMARPLPKDLGATTPFLYCWRDREKLYSLFERITGGRMLYNYLRIGGVRNDLPEGILGTPQDGEDKADKTIWGFINYFDSYVYPEWKALVTGNRIFQYRTKNIGVLTAEQAIAYSCSGAVLRGSGVKWDLRKNLPYAIYDRFEFDIPVGQNGDSFDRWWVRQEEMYQSSRIVKQCLEWLAENPGPVMARPLPKAKP",
    "response#3": "DIGAMTVFLYAFREREQILQFFEKICGARLTVSYPRIGGVRVDIKEHVLDEIYKFMDLMLIRVDEYETLLTENRIWIARTRGVGVIAPEDAVLLGLTGPALRGSGVYYDIRKQIPYDAYSEIDFEVPLGEKGDTYDRYLCRIREMRQSVLIVKQCIEKMPEGKILSDKSPDIDLPHQAKRKIEPGDSLWNGFIAFSEEKQEIMPKGEIYSAIEAPKGELGFYIVSDGSGRPYRMRVRAPSFIHISAIPKLCEGHLLADVIAIIGTLDIVMGEADCSA"
  },
  {
    "reference": "MPTISCDKEELYKALGREYTTQEFDELCFQFGIELDEDTTNDPERSPSERPSLKIDIPANRYDMLCLEGIAQALNVFNRRMATPQYKLLPSTTSLTISPETSEIRPYAAAAILRGVKLDPIRYQSFIALQDKLHANLCRNRTLVAIGTHDFSVMEGPFTYEALKPEEINFVPLNQTQEINGSNLLEFYKDSKHLSRYLHIIANSPRYPVILDAKRRVCSLPPIINSEFSKISVDTRDIFIDVTATDKTKLEIVVNMMTTMFSCYCEEPFTIEPVNIISEHNGCTRVTPNLNPTCFKADIDYLNEACGLSLPEDEICHLLTRMMLTAKPNPNDSKTLLVYVPPLRADILHQCDIMEDLGIAYGYDNLKHTYPAHSVTFGKPFEVNRLADIIRNEVAYAGWSEVMPFILCSHDENYAWLRKTDDSKAVQLANPKTLEFQVVRSSLLPGILKTVRENKNHALPIKIFEVSDVAFCDYSRERMTRNERHLCAIFAGLNSGFEQIHGLLDRVMLMLNTKRIMNPKDSDAVGYWIEAEDDSTFFPGRCAAVYYRKDFGTAGIRVGVFGVLHPLVLEKFELTSAASAVEIDLTLWV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must be able to phenylalanine-tRNA ligase activity. The designed protein sequence is ",
    "response#1": "VRPYIVGAIVRGMDLQPGNALRRFLNSQTKLHDDLCEKRTAATIATHDLQAVRGPLLYAARPPEDLKIVPLGRKEAKAKELVRQLQLEAEEQRKQKKRQSVSGLHRYLHLLDGKENYPCLVDAEGDVISFPPITNSEKTKIKKTTCNLFLEVTSATSLQLCKDIMDSLILRMAELSR",
    "response#2": "VQYAHARVCSVLRQAQEKGYKYDQAHGLAELACLDDEHSLAVMLELSRYPEVVEIAGQALEPYQIAQYLRELAHAFHTWYHNSKVLVDAAAERDAKLTLAVATQQVLANGLELLGVSAPEKC",
    "response#3": "SIKSLMDLQEDLHWGLGRNRKKVSIGVHDLSNVKPPFRYMAVDPGFKFVPLDYTEKMSMTEILEKHPKGTRFAHLVRGFKKYPIILDSDDNVLSFPPIINGTLTSVTESTTDLFIDVTGLGQ"
  },
  {
    "reference": "MPDEIRIFSGNSNRPLAARIASYIHQPLSECQIRRFSDHEIFVQIDENIRGMDVFIIQSTNPPAENIMELLIMIDAARRASARRITAVIPYFGYARADRKDQPRVSIVSKLLANMITVAGANRVVLMDLHASQIQGFFDIPTDHLYSARVFNRHFLKEGAADAVVVSPDVGSTKIARAFAAKIDATLAIIDKRRPEQNKAEVMNIIGDIKGKNVIIRDDMVDTGGTLIEAAIALKNAGAKSIVACCTHAVVSGNAVARLTDSPIEKLYITDTIDNSAKNLSDKFVILSVAELLGESILRIHTERSVSTLFD",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The presence of Ribose-phosphate pyrophosphokinase N-terminal domains is critical for the interaction of the designed protein with its intended targets.2. The protein should have a high affinity for D-ribose 5-phosphate, Mg(2+), ATP.3. The active site of the protein must be engineered to enable effective catalysis of ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-   diphosphate + AMP + H(+).4. The protein must exhibit the following characteristics: Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P)..5. The protein requires Mg(2+) to activate a critical catalytic site.6. We require a protein that can specifically bind to the metabolites produced in the 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1, 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, Metabolic intermediate biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGADRIMSVDLHAVQVQGFFDIPVDHLFAPLLADYFLRNNLEEGAVVVSPDHGGVNRARKLAEFLDCPIAIVDKRRPKANVAEVMNIIGDVKGKRAIIIDDMIDTAGTITLAAQALIDAGATEVYASATHAVLSGPAIKRLNDSPIKNLVLTDTIPLREEQSFPRLTVLSVAPLLAEAIHRIHRGLSVSALFAEH",
    "response#2": "MKIFAGNAIPELAKRIASKLYTNLGNAKISRFSDGEVSVQINENVRGDDIFIIQSTCFPTNDNLIELIVMVDALRRASAARVTAVIPYFGYARQDRRVRSARVPISAKVIADFLSSVGADRIMSVDLHAEQIQGFFDVPVDHVFAPVIVDYLANKQLSDLVVVSPDVGGVARARAFAKKLNGAPLAIIDKRRQAHNVTEVLNLIGDVHGKTAVLVDDMIDTAGTILEGAKVLRKKGARQVYACATHAVFSEPAISRLSSGILEEVIVTNTIPIPAEKKFSQLKVLSVAPLIAEAIHRIHSNESVSALFVD",
    "response#3": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGADRALSMDLHAVQVQGFFDIPVDHLFAAPVLIKAIRERGLEDIVVVSPDVGGIKMSHAYAKTLGVPFAIVAKNRVSAEEVEALNIIGDVRGKNVLLVDDLTETAGTLTAAAKLLQEHGAQSIYAGVSHAVLGEKGHSRIESSPIIELFSTDTIPLSEAEKKYPRIKVISVAPLMGEAINRTSMETSVSSLFD"
  },
  {
    "reference": "MDQKGSQRALLAQTINEIVKLLIEAHNQKKDVNLNRLKCIVAQKNGLSFQPKLVDIIAGVPSDYKDSLLPKLKAKPVRTASGIAVVAVMSKPHRCPHINFTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYNPYLQTRGRLNQLMQLGHSVDKVEFIVMGGTFMSLPEDYRDFFIRNLHDALSGHTSASVEEAVAYSERSKMKCIGITIETRPDYCLPRHLNDMLLYGCTRLEIGVQSTYEDVARDTNRGHTVKSVCETFHMAKDTGYKVVIHMMPDLPNVGLERDKEQFLELFESPAFRPDGLKLYPTLVIRGTGLYELWKTGRYQSYPPSVLVDLIATILSLVPPWTRVYRVQRDIPMPLVSSGVEHGNLREHAMAKMKELGLKCRDVRTREVGIQEIHNKVRPEDVELIRRDYTANGGWETFISYEDPKQDILIGLLRLRKISDKAHRPELKGNVSVVRELHVYGSVVSVADRDPKKFQHQGYGSLLMEEAERIAREEHGSDKIAVISGVGTREYYRKLGYELDGPYMSKMLDSAAA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must bind to [4Fe-4S] cluster in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "VCKRCKEVHVGPSGHKARLCGVFKYESWRGTHYWEKAGVNDLVPEKMVWHRRPQDPVVLVDEGRSYYGHAPAIVSLCSHTGAIVPE",
    "response#2": "VCKRCKEVHVGPSGHKARLCGVFKYESWRGTHYWEKAGVNDLVPEKMVWHRRPQDPVVLVDEGRSYYGHAPAIVSLCSHTGAIVPI",
    "response#3": "VGANALMAWEKVRAGVKKLLLVYPSKVCKRCKEVHVGPSGHKARLCGVFKYESWRGTHYWEKAGVNDLVPEKMVWHRRPQDPVVLVDEGRSYYGHAPAIVSLCSHTGAIVPVKYACKL"
  },
  {
    "reference": "MRRWICCGDKKGDSDLSNEEVHLKSPWQNSEANQKNQKPQAVVKPEAQKEALPIEVPPLSVDEVKEKTDNFGSKSLIGEGSYGRVYYATLNDGKAVALKKLDVAPEAETNTEFLNQVSMVSRLKHENLIQLVGYCVDENLRVLAYEFATMGSLHDILHGRKGVQGAQPGPTLDWLTRVKIAVEAARGLEYLHEKVQPPVIHRDIRSSNVLLFEDYQAKVADFNLSNQAPDNAARLHSTRVLGTFGYHAPEYAMTGQLTQKSDVYSFGVVLLELLTGRKPVDHTMPRGQQSLVTWATPRLSEDKVKQCVDPKLKGEYPPKSVAKLAAVAALCVQYESEFRPNMSIVVKALQPLLKPPAPAPAPVPES",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Probable tyrosine-protein kinase involved in oxidative burst- mediated signaling leading to specific genes expression.2. The protein can be targeted to plasmodesma, peroxisome and perform ATP binding.3. The ATP binding site should be stable and able to withstand changes in environmental conditions.4. The designed protein should contain one or more Protein kinase domains that are essential for its function.5. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "RKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFL",
    "response#2": "WRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFL",
    "response#3": "PTRCQVLGRGAYGTVFKAIYRDRSVAVKIIRAQAASTLHNESHLLNLEHRNIVRLLKLESAADFGLVIMECPRGQSLQRIVDTLALPLMHRVLITLDVVAALRYCHSQNVLHLDVKPTNILVALGTKSSITCNSSKIKVKRSYICKLCDFGSSIEMGEFCAWQEPSVAKGTLRYMSPEALRSDTLTEASDIYSLGITMWQLQARRLPYHTLDCNETIAYQVVKHELRPDNYHQLKILALDSPIDCDWDLAHESTANVICRRANTSARRNLSLDPSYTVGRDLKKKRHRNRLALHFDSPAPEGSACSESAYSQLYKSCWVSAPELRLSSIQLKHELEFYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFPTRCQVLGRGAYGTVFKAIYRDRSVAVKIIRAQAASTLHNESHLLNLEHRNIVRLLKLESAADFGLVIMECPRGQSLQRIVDTLALPLMHRVLITLDVVAALRYCHSQNVLHLDVKPTNILVALGTKSSITCNSSKIKVKRSYICKLCDFGSSIEMGEFCAWQEPSVAKGTLRYMSPEALRSDTLTEASDIYSLGITMWQLQARRLPYHTLDCNETIAYQVVKHELRPDNYHQLKILALDSPIDCDWDLAHESTANVICRRANTSARRNLSLDPSYTVGRDLKKKRHRNRLALHFDSPAPEGSACSESAYSQLYKSCWVSAPELRLSSIQLKHELEF"
  },
  {
    "reference": "MPNNTLKQGSKIENVSPSKGHVPSFWKQFINNPKSMSSENITVPRSPTSLSRNAQPTTLKRPPLSSRPYSYNTPTKDRKSFSKSAKQNNNNNNANSGTSPHAEFKNYRDMFLSNRNGFTGRVFGVTLAESLSVASAEVIVQSELVSFGRIPIVVAKCGAYLKANGLETSGIFRIAGNGKRVKALQYIFSSPPDYGTKFNDWETYTVHDVASLLRRYLNNLAEPLIPLSLYEQFRNPLRSRPRILRHMLTHEVSHPNANKTNNVTVKSSRQNYNDDGANDGDIEKEDAKDDEEKRRRKIRHKRRLTRDIRAAIKEYEELFVTLSNDTKQLTIYLLDLLSLFARQSQFNLMSGRNLAAIFQPSILSHPQHDMDPKEYELSRLVVEFLIEYSYKLLPHLLKLAKREQQERLSTENKKNNGDKQKTDPIEIPKITSSDSPPIVSSNKNPPAIDNNNKLDHTTLSPISTSIPENSSDLQTSKMLKPPKQRRPHSKSFGSTPVPPDVIASNKRRTSLFPWLHKPGILSDTGDNGDLTATEAEGDDYEEENVDPYGQSPSSVHSGSLPKQHYLPIPRMNRSLSGNSTNSSFNTRPISMILTSGNDNSADQLELLSNTHSNNERSNALPLTEDDGDERNSRSRKRESWFQRLTSRSGSANRA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.2. The designed protein must have at least one Rho-GAP domain.3. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cell cortex that is critical for regulation of actin cytoskeleton organization, negative regulation of protein kinase C signaling. The designed protein sequence is ",
    "response#1": "MSLKRKEKGVIFGSPLTEEGIAQIYQLIEYLHKNLRVEGLFRVPGNSVRQQILRDALNNGTDIDLESGEFHSNDVATLLKMFLGELPEPLLTHKHFNAHLKIADLMQFDDKGNKTNIPDKDRQIEALQLLFLILPPPNRNLLKLLLDLLYQTAKKQDKNKMSAYNLALMFAPHVLWPKNVTANDLQENITKLNSGMAFMIKHSQKLA",
    "response#2": "YTLPALLSKKEETFLNEAGFNFVRKCIESVEARGINTLGLYRIGGVNSKVQRLMTSVFAAKAPADLDLDPDTWDNKTITSGLKNYLRCLAEPLMTYRLHKDFIMAVKSDDQNYRVCAVHALVYKLPDKNKEMLNLLIKHLHVVSTHSQKNLMTVSNLGVIFGPTLMRSQEETVAAMMNIKFQNIVVEILIENYDKII",
    "response#3": "KQIPHVVEACCSFIEKHGLSTVGIFTLEYSEKRVRKLREEFDQGLDVVLDDSQNVHDVAALLKEFFRDMKDSLLPDDLYMSFLQTATLKPQDQLSALQLLVYLMPPCHSDTLERLLKVLHKVAENCEDSIGIDGQLVSGNRMTSTNLALVFGSALLKKGASAKRESRKTRLGIDHYVASVSVVRAMIDNWR"
  },
  {
    "reference": "MRVRGILRNYQHWWIWGILGFWMLMNCNVGGNLWVTVYYGVPVWKEAKTTLFCASDAKAYETEVHNVWATHACVPTDPNPQEIFLKNVTENFNMWRNDMVDQMHEDVISLWDQSLKPCVKLTPLCVTLRCRDVTNNGSYNGSAEEIKDCAFNVTTVVRDRKQEVHALFYRLDIVPLNETNSSKNFSEYRLINCNTSAITQACPKVTFDPIPIHYCTPAGYAILKCNDKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENITDNVKTIIVHLNQPVEIVCIRPNNNTRKSIRIGPGQTFYATGEIIGNIRQAHCIITRWNETLQKVGKKLAEYFPGKSINFRPSSGGDLEITTHSFNCKGEFFYCNTSGLFNGTYMPNGTMDSPSTIPCRIKQIINMWQEVGRAMYAPPIEGNITCQSNITGILLVRDGELNRTEEIFRPGGGDMRNNWRSELYKYKVVEIKPLGIAPTEAKRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNRSQDEIWKNMTWMQWDREISNYTNTIYRLLEDSQNQQERNEKDLLALDSWKSLWNWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTLIPNPGEPDRLGRIEEEGGEQDKTRSIRLVNGFLALAWDDLRNLCLFSYHRLRDFILVAARGVGLL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.2. The protein should be optimized for stability and solubility when expressed in host cell endosome membrane, virion membrane where it will be involved in fusion of virus membrane with host plasma membrane, fusion of virus membrane with host endosome membrane, positive regulation of establishment of T cell polarity, viral protein processing.3. The protein must exhibit the following characteristics: The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes.4. Design the protein with a signal peptide incorporated.5. The designed protein should contain at least one YXXL motif; contains endocytosis signal motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPAWKDAVTTLFCASDAKAYDKEVHNVWATHACVPTDPNPQEIKLVNVTEEFNMWKNPMVEQMHTDIIGLWDQSLKPCVQLTPLCVTLDCHDNITSDNITNNGNTTNSPNITNEMGGEIKNCSFNMTTELRDKRKKVHSLFYRLDIVEIGNSSSSNNHGQYRLINCNTSAITQACPKVTFEPIPIHYCAPAGFAILKCTEKRFNGTGPCRNVSTVQCTHGIKPVVSTQLLLNGSLAEENVTIRSENITDNAKTLIVQLVKPVNITCIRPNNNTRKGIGIGPGQTFYAADAIIGNIRHAYCVVNKTEWNKTLQQVVHKLREHWNKTIIFAPSSGGDIEITTHSFNCGGEFFYCNTSGLFNSTWHVNNTSSLVNSTGNITLPCRIKQIINMWQRVGQAMYAPPIPGIIKCESNITGLLLIRDGGINNNSMNETFRPGGGDMRDNWRSELYKYKVVQIEPLGVAPTKAKRRVVEREKRXLGAGFLEWGRLSXWLXGXXXTTXXXLWVTVYYGVPVWREADTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEMDLENVTEDFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLDCHNVTSSNSSXSTNXXXXTSNVTEKTNVTEEIRGEIKNCSFNVTAELRDKERKVHSLFYKFDVVPINGSSNTSYRLINCNTSTITQACPKVSFEPIPIHFCAPAGFAILKCKEKEFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEGKVVVRSENFTNNAKIILVQLADPVKINCTRPNNNTRRGIYIGPGKTFYATDKIIGNIRQAHCNISRQKWNETLYKVAKQLKXHFGNKTIIFDKSSGGDLEITMHSFNCGGEFFYCNTSGLFNSTWXNNTTWDNNTESNDTITLQCKIRQIVRMWQRVGQAMYAPXXQGVIRXESNXXGLLXTRDGGNISSXNETFRPGGGDMRDNWRSELYKYKVVQIEPLGVAPTRAKRRVVQREK",
    "response#2": "LWVTVYYGVPIWREAKTTLFCASDAKAYEREAHNVWATHACVPTDPNPLEMVLENVTENFNMWENDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCSNVNANATYNNASATYNDSSETGLKNCSFNTTTELKDKKHQEFALFYRLDIVPLKENNGSSSENNVDSSEEYRLINCNTSTVTQACPKITFDPIPIHYCTPAGYAILKCNNKTFNGTGPCHNVSTIQCTHGIKPVVSTQLLLNGSTAEEEIIIRSKNLTDNTKTIIIQLKESIEIVCTRPNNNTRRSIRIGPGQAFYATNGIIGDIRQAHCNISEKRWNKTLQNVGKKLREHFPNKTIVFNSSSGGDLEITTHSFNCRGEFFYCNTSGLFNGTFDGTESNSTSNATITIPCRIKQIINMWQGVGRAMYAPPIAGNITCKSNITGLLLVRDGGNTDNTNGTEIFRPGGGDMKDNWRSELYKYKVVEIKPLGIAPTGAKRRVVKREKRXXXXXXXXXXXXXXXXXLWVTVYYGVPVWKEAATTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEIVLENVTEKFDMWKNYMVEQMHTDIISLWDQSLKPCVKLTPLCVTLHCKNVTATNE",
    "response#3": "LWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLGNVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVELTPLCVTLHCTDLKKNATENGTFTNNNSSGGRMEEGEMKNCSFNITTNIRDEVKKEYALFYKLDIVPIDNGNDNGSTSYRLISCNTTVIKQACPKVSFEPIPIHYCAPAGFAILKCNEKKFNGTGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVVIRSENFTNNAKIIIVQLNESVEINCTRPNNNTRRGIHIGPGRAFYATGDIIGNIRQAHCNISETKWNYTLNLVAKKLREQFGENKTIVFNQPSGGDPEIVMHSFNCGGEFFYCNSTPLFNSSWPFNRTWNDTEGSNNTERNITLQCSIKQIIRMWQKVGKAMYAPPIPGLIRCSSNITGLILTRDGGNNNTNGNETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRXLGXLWPRS"
  },
  {
    "reference": "MRLCFASNNAHKLDEIRPLLPAGIELLSLADIGCQEELPETQDTLEGNARQKARYVWDHYATPCFADDTGLEVAALNGAPGVYSARYAGPQRDAADNIQKLLLELQNQTDRAAQFRTVVALVLSETEEHLFAGSVPGLITEAPRGSGGFGYDPVFVPTEGDGRSFAEMPLADKNELSHRARAVKKLVAFLNGLTDGNA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must be optimized for nucleotide binding, dITP diphosphatase activity, metal ion binding.2. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.3. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.4. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+) binding. The designed protein sequence is ",
    "response#1": "MGHRKLFFE",
    "response#2": "MNIILANPRGFCAGVDRAISIVERALELYGAPIYVRHEVVHNRYVVDGLRDRGAIFIEELSEVPDDNVILFSAHGVSQAVRNEARSRGLRVFDATCPLVTKVHMEVARASRKGKEAILIGHAGHPEVEGTMGQYDNPEGGMYLVESPDDVWKLQVKNEENLCYMTQTTLSVDDTSEVIDALKARFPKIVGPKKDDICYATQNRQDAVRDLASDADVVLVVGSKNSSNSNRLAELAQRVGKPAYLIDSASEIKEEWITGISSVGVTAGASAPDILVQQVIDRLTALGANQVETLTGREENIVFEVPKELRVEVK",
    "response#3": "MMKRNIFLII"
  },
  {
    "reference": "MAFLKKSLFLVLFLGMVSLSICEEEKRENEDEEKQEDDEQSEMKRGLWSTIKNVAAAAGKAALGALGEQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Possesses a potent antimicrobial activity against Gram- positive and Gram-negative bacteria; Probably acts by disturbing membrane functions with its amphipathic structure ..2. The protein must contain a signal peptide for proper functionality. The designed protein sequence is ",
    "response#1": "ISGLKNVGKEVGMDVVRTGIDIAGCKIKGEC",
    "response#2": "LAFLKKSLFLVLFLALVPLSICEEEKREGENEKEQEDDNQSEEKL",
    "response#3": "MQLKKSLLLLFFLGTINLSFCEEERDVDQDERRDDPGERNVQVEC"
  },
  {
    "reference": "MNILKEAKPNILVLGDLMIDHYLWGSCERISPEAPVQVVDVAKETTVLGGAGNVINNLKALGAEVSVAGVIGDDANGEELREMLREIAVDTQKLIIQAGRKTSKKSRVIAVSQQILRYDKESKDAIEDVSVEKILDSIKEGIKEYDALILSDYGKGVLTETLCQGVIALANANGVKVLVDPKGSDFSKYRGAYLLTPNKKEAQFATGMKIDDEGSLKEALLTLKSECDLDISLITLSEDGIAIYDDELKRFPTVAKEVFDVTGAGDTVIASIAYALSAGKDIDETAQFANLAAGVVVGKIGSATVTIDEIEEYEASLHKSTSDAHIKTFEEIDAIVQRYKTQGKKVVFTNGCFDILHVGHVKYLQIAKSFGDVLIVGLNSDASVSRLKGPTRPVNVAEDRAYLLAALEAVDFVVPFEEDTPYELIKMIAPDVLVKGGDYEGKTVVGTEFARELKLVDFVDGKSTTKTIEKIQGQTC",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Ideal protein should have a binding affinity for key enzymes in the Nucleotide-sugar biosynthesis, ADP-L-glycero-beta-D-manno- heptose biosynthesis pathway to enhance the overall efficiency of the pathway.2. A protein with phosphotransferase activity, alcohol group as acceptor functions is required to enable ADP-L-glycero-beta-D-manno-heptose biosynthetic process. The designed protein sequence is ",
    "response#1": "MTNGCFDILHAGHVSYLQQARALGDRLIVAVNTDASVKRLKGEGRPVNSEDRRMTVLAALASVDWVVPFSEDTPQRIISRLLPDLLVKGGDYKVEDIAGGSEVMAAGGQVKVLGFEDGISTTAIIL",
    "response#2": "MTNGCFDILHAGHVSYLQQARALGDRLIVAVNTDASVKRLKGEGRPVNSEDRRMTVLAALASVDWVVPFSEDTPQRIISRLLPDLLVKGGDYKVEDIAGGSEVMAAGGQVKVLGFEDGISTTAIILS",
    "response#3": "MTNGCFDILHAGHVSYLQQARALGDRLIVAVNTDASVKRLKGEGRPVNSEDRRMTVLAALASVDWVVPFSEDTPQRIISRLLPDLLVKGGDYKVEDIAGGSEVMAAGGQVKVLGFEDGISTTAIIL"
  },
  {
    "reference": "MNIDVVQLLDQNPILLIFVVLAIGLAIGKIRFGNLQLGNSIGVLITSLIMGHLGFSFNAEALTIGFMLFIYCVGIEAGPNFFGIFFRDGKHYFILSMTVLVAAVSLTYGLSHYFGLDFGLSAGMMAGALTATPVLVGAQDALNSGLATIPRNMDFSLVLENLSVGYAMAYLIGLISMIMFAKLLPKLQKQNLSDSAQQIAQERGLGNSSQRKVYLPIIRAYRVGPELIDWTDGKNLRELGIYRQTGCYIERIRRNGILAHPDGDAILQEGDEIALVGFPDSHARLDPSFRNGKEVFDRNLLDLRIVEEEIVVKSDAIAGKRLSDLNLSEFGCFLNRVVRAQIEMPMDLDIVLAKGDVLQVSGEKSRVHGLAEKIGFISIHSQMADLLAFCSFFILGIMFGLVTMTFGQVSFSLGNAVGLLLSGITLGFLRANHPTFGYVPQGALNMVKDLGLMFFMVGIGLSAGGKMFEHLTQVGPQVIGLAFIVSVVPVVIAYLVGAYILKMNRALLFGAIIGARTCAPAMDVVNEYAKSTIPALGYAGTYAIANILMTLAGTILIILS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should contain a Helical membrane-spanning region.2. The designed protein must have at least one RCK C-terminal domain.3. A protein that contributes to potassium ion transport by functioning in plasma membrane with cation transmembrane transporter activity. The designed protein sequence is ",
    "response#1": "LAYQSLGVVYGDLATSPLYVYKSTFAEDITHSETNEEIFGVLSLIFWTLTLIPLVKYVFIVLRADDNGEGGTFALYSLLCRHARISSLPNFQLADEDLSEYKKNSGENPMRLKVPGWSLKNTLEKHKFLQNMLLVLALIGTCMVIGDGVLTPAISVFSAVSGLELSMSKQQHQYVEVPVVCAILILLFSLQHYGTHRLGFVFAPIVLAWLLCISTIGVYNIFHWNPHVYKALSPYYIYKFLKKTRKRGWMSLGGILLCITGSEAMFADLGHFTQLSIQIAFTFAVYPSLILAYMGQAAYLSKHHVLQSDYRIGFYVSVPEQIRWPVLAIAILAAVVGSQAIITGTFSIIKQCTSLGCFPKVKIVHTSSRMHGQIYIPEINWTLMLLCLAVTVGFRDTKHISNASGLAVITVMLVTTCLMSLVIVLCWRKSSLYALAFIFFFGTIEVLYFSASLIKFLEGAWVPVALSFIFLLIMYVWHYGTLKRYEFDVQNKVSINWLLGGPGTAWHQFDVRAFRVRERGPAVGKTVQEAEALIPDQRVFILRLRQEGKIVDATSDAVIHAGDVVAVAGRRDVLVQLIGEQAR",
    "response#2": "GIGVINTELVSGIPAIFSHFITNLPAFHQVVVFLCVKSVPVPHVKPEERFLVGRVGPKEYRLYRCIARYGYRDVHKDDVEFENDLICSIAEFIRSDKPLNYSPDPENESGINERLTVVAASSSNLEGVQIYEDDGSDKQEPSSSSEVIMVAPSPRFKKRVRFVLPESARIDRSAEEELTELTEAREAGMAFIMGHSYVRAKSGSSVMKKIAINFGYDFLRRNSRGPI",
    "response#3": "GIGVINTELVSGIPAIFSHFITNLPAFHQVVVFLCVKSVPVPHVKPEERFLVGRVGPKEYRLYRCIARYGYRDVHKDDVEFENDLICSIAEFIRSDKPLNYSPDPENESGINERLTVVAASSSNLEGVQIYEDDGSDKQEPSSSSEVIMVAPSPRFKKRVRFVLPESARIDRSAEEELTELTEAREAGMAFIMGHSYVRAKSGSSVMKKIAINFGYDFLRRNSRGPC"
  },
  {
    "reference": "MPGGRASSSYGLSVTDDKDARIWERIKDHPCFSEQAHHYFARMHVAVAPACNIQCNYCNRKYDCTNESRPGVASVKLTPDQALRKVLAVASKVPELSVIGVAGPGDACYDWRKTAATFEGVAREIPDIKLCISTNGLALPDHVDELADMNVDHVTITINMVD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must be able to perform 4 iron, 4 sulfur cluster binding under specific conditions. The designed protein sequence is ",
    "response#1": "ICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYNPYVQARSRIDQLKRLGHSVDKVEFILMGGTFMSLPAEYRDFFIRNLHDALSGHTSANVEEAVAYSEHSATKCIGMTIETRPDYCLGPHLRQMLIYGCTRLEIGVQSTYEDVARDTNRGHTVAAVADCFCLAKDAGFKVVAHMMPDLPNVV",
    "response#2": "WENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKRLGGDPAKINPLVPVDLVIDHSVQVDVSRTVDALEQNQKIEFHRNHERFSFLKWGAQAFDGLFIAPPGSGIVHQVNLEYIAREVMNGTGNLLYPDSVVGTDSHTTMINGLGVCGWGVGGIEAEAVMLGQPMSMVLPEVVGYKFVGKLPDIATATDLVLTVTNELRKKGVVGKFVEFYGEGVSTLSVQDRATISNMAPEYGATMGFFPADENTIDYLASTGRSNTKIEYIKNYLSSQGLMCNYKSQSHPIFTTTMELDLSTVVPSLSGPKRPHDRISLNSMKQDFNSCLSSPVGFKGFGLTADQIQKKATFTFKDKQYTIGHGAVTIAAITSCTNTSNPSVMLGAGLLAKNAVEHGLEVAPYIKTSLSPGSGVVTEYFSHSGLQEPLNKLGFDLTGYGCMTCIGNSGELAEPLAEAITKEDLVVAGVLSGNRNFEGRIHPLLRANYLASPPLVVAYALAGHITT",
    "response#3": "DLNAIKTAACRKYGLARAPKLVEMIAALPDSERETLLPKLRAKPVRTASGIAVVAVMSKPHRCPHIATTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYNPYVQARSRIDQLKRLGHSVDKVEFILMGGTFMSLPAEYRDFFIRNLHDALSGHTSANVEEAVAYSEHSATKCIGMTIETRPDYCLGPHLRQMLIYGCTRLEIGVQSTYEDVARDTNRGHTVAAVADCFCLAKDAGFKVVAHMMPDLPNVGVERDMESFKEFFESPSFRADGLKIYPTLVIRGTGLYELWKTGRYRNYPPEQLVDIVARILSMVPPWTRVYRVQRDIPMPLVTSGVEKGNLRELALARMDDLGLKCRDVRTREAGIQDIHHKIKPEQVELVRRDYTANEGWETFLSYEDTRQDILVGLLRLRKCGKNVTCPELMGKCSVVRELHVYGTAVPVHGRDADKLQHQGYGTLLMEEAERIARREHRSNKIGVISGVGTRHYYRKLGYELEGPYMVKHY"
  },
  {
    "reference": "MPHNFKPGDLVFAKMKGYPHWPARIDDVKDGAVKPPPNKCPIFFYGTHETAFLAPKDLFPYDKYKDKYGKPNKRKGFNEGLWEIQNNPQASYSLPPASVSSSDSDVPEEKSTARSDEEEKQEASQPILPTASVSASDEEGSEKEGLKRKERITTAPSAKRTKHSSSEQEPDSASSSKEENSDSDQDFTPEKNTPRIQRRITNVGEKNKVLAESESDSKSESEDEKKELKKSPSSSSASSPSLSSSDSETPVKKTPRGRRPAEKPAPKPRGRGRKAEPIPSSDSSDSDSSVDRISEWKKRDEERKRELEERRKKEQEEQLRRLREEEREEDEKKKREKAEKGDKSDSDSDSSKSEVTAPPKPRKSSSSSDSEEDKKQVKEVKPVASEIKKGKKDKVRAISDDSDSDKKVKKTIKKPRPAESARKTNQKEKRGERPRGRPSKVEKEKKKPEMINSRRVVKKEPTVEERLQKLHSEIKFALKVDNPDIKKCLDALEELGGLQVTSQILQKNTDVVATLKKIRRYKANQRVMDKAAEVYSRIKARILGPKSESQQKIAEKVNTAEKGPEDENQTGKAGEDMDASMNGDFLSQRTETAGDKEQELEGLNLDNKAEMETKQNNHAEHNNNPTEETFEPRPISSENQNS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Design the protein with a signal peptide incorporated.2. For general function, the protein need meet that Promotes the repair of DNA double-strand breaks (DSBs) through the homologous recombination pathway by facilitating the recruitment of the DNA endonuclease RBBP8 to the DSBs .3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.4. The protein design should be able to enhance the efficiency of muscle organ development. The designed protein sequence is ",
    "response#1": "IEFPDIINKLWQKAESFPGKQLALKVRPLSDSKIIEEKLLEVEEGLSYLRFKTVDLSVLSDFSEIFLKLSKESMLTGQEIYRLGQLLKVSKDTFFEISRGAFPRLKQIVKLLFFDEALVKDIERSFWPEGTVKDEASPELKRIRGQIARLKDKMREAVEKYLKEPELAKYLQEPLISVRGDRFVLPVKASYKSQVPGIIHDRSNTGQTLFIEPYSAVEAGNELKTLELQEKEIIEKILKDFTQRLACNLTEIKRTYELLGEIDLIVAKARLALELDAYKPRISENGVLSFKQARHPLLGKA",
    "response#2": "QELLTAVRDLHDTALQELQAKIAKLKKERCLDAQKLSEFHSKNQHLREQQKIQQEKIRQLEDRLRSGPCDRCTVKEKQIKKTNTELEDNNQRNLSVISELEAERKTLTDENRRLSLEA",
    "response#3": "QELLTAVRDLHDTALQELQAKIAKLKKERCLDAQKLSEFHSKNQHLREQQKIQQEKIRQLEDRLRSGPCDRCTVKEKQIKKTNTELEDNNQRNLSVISELEAERKTLTDENRRLSLEI"
  },
  {
    "reference": "MTENLQSESIPHEILPKEPFDLPMNNLKSSPKNKDSEKRINNSIAESEQVVDSALSNPETNANEDIIAPQLPSQNSEIIEKNSPVNKLNSSTSLTTHQLASLPKLEVTDHDNVSEAETVVLNEDEEKETSLVGSVSVTEDLGDSSAIGRTILVNNSVEPQMENTANITIVSPSLKESDFESEEKATNDNNGLIETNHNSKLEESSEHEEEEDEESNIERTEDSDHQIPQRGGTLEAPRKGGPRSGVGSRKRKRATVSRKWSTNSESKIKRVALETSQEESDREIADRRSASEQAHEADDEKAIKRKEAFDALLNIETEFTFLRNRLYGKKLLKLNEHEEMIQNETHERFNACIDLITERRDDRVRLATENLMKQLGNIKNVMDYVTKQRKYQLLFDKRRIRQALLTKIATKCFQLLNKQKSVHDPTYITQKTMSYRQSALLQKQRIEYEAAVLCELNSFAGFPTAPIIETASFDDIRNDLLEMGCLSENQD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should be able to enhance the efficiency of negative regulation of transcription by RNA polymerase II, chromatin organization.2. The protein design should emphasize Basic and acidic residues compositional bias.3. For general function, the protein need meet that Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events . The designed protein sequence is ",
    "response#1": "RKGSLMAPHVLKVHGSSWPAELIELCQMADAKLLKGEIEVPDTWNSGDIYLSSKTIKALQGTIGAIETGVDSIFKGPSAEHISNRAFVAIRPPGHHCHYGTPSGFCLLNNAHVAIEYAYDTYNVTHVVVLDFDLHHGDGTQDICWKRAGFKPEEEPEDSSYDDFGKKFAEFPKVGYFSMHDINSFPTESGFATKENIKNASTCIMNSHDLNIWNIHLSKWTTEEEFNVLYRTKYRTLFAKADEFFRSAKLEMNQQGRPFKGLVVISAGFDASEFEQTSMQRHSVNVPTSFYTTFTKDALKLAQMHCHGKVLSLMEGGYSDKAICSGVFAG",
    "response#2": "HPMKPQRLVVCNDLVVSYEMPKYMTVVESPKLDAADISVFHTEDYVNFLQTVTPKLGLTMPDDVLRQFNIGEDCPIFAGLWDYCTLYAGGSVEGARRLNHKMNDIVINWPGGLHHAKKSEASGFCYVNDIVLGILELLKYHKRVLYIDIDIHHGDGVQEAFNNSDRVMTVSFHRFGQYFPGSGSIMDKGVGPGKYFAINVPLMAAIRDEPYLKLFESVISGVEENFNPEAIVLQCGSDSLCEDRLGQFALSFNAHARAVKYVKSLGKPLMVLGGGGYTLRNVARCWALEYGFFTVGDCVLMRPSDAGKAPYVARVEKIEADARNNVKVHCRWYYCPEESHGGRRQLHGAKELFLSDHFDVQSAHTIEGKCIVHTFKNYTRLENVGVEDYYCIFDYKAATGAFTPDGTKPIHVVEAHNSEVNCLSFNPFCEFLVATGSTDKTVALWR",
    "response#3": "HPEHAGRIQSIWSRLQERGLRSQCECLRGRKASLEELQSVHSERHVLLYGTNPLSRLKLDNGKLEGLLAQRMFVMLPCGGVGVDTDTIWNELHSSNAARWASGSVTDLAFKVASRELKNGFAVVRPPGHHADHSTAMGFCFFNSVAIACRQLQQQGKASKILIVDWDVHHGNGTQKTFYQDPSVLYISLHRHDDGNFFPGSGAVEEVGAGSGEGFNVNVAWY"
  },
  {
    "reference": "MNSDQPLNTSLQPASLSREMLLEAVASWHLPQQDFEAGTSPSVEHITNDSRKVTSGSLFVAIPGAELDGWQFVPKALTAGAKYIVGEHVLSQEEKALFEQVDASYIAVSSAHEALAALAQALWHYPSKHLRLVGVTGTNGKTSTATMLHRLFERLGYKSGLIGTVENRIGNEIIPATHTTPDPIALAQLLYRMQQAGCSHVFMEVSSHAAHQRRIGSLEFVGGLFTNLTRDHLDYHGTMQEYIRAKKMFFDQLSPNAFALVNADDRNGSVMLQNTSAKKYTYGLKSFADFKAKVVEGDFRGTELLLNDRSVWIPLVGAFNAYNITLVYAAARLLLPDLEQDVLLQALSTIETAEGRFEVISEGGRIGIVDYAHTPDALIKVLETIRPLVPQGGRVITVVGAGGNRDRGKRPIMAQEAFRRSDLLILTSDNPRDEDPQAIINEMMAGLTPEEQAQCLTNVDRRSAIATACRLATPRDVILVAGKGHETYQEIHGVRHHFDDREELRLALRNTPLQAN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a binding site that is specific to the target UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP, meso-2,6-diaminoheptanedioate ligand.2. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.3. The presence of Mg(2+) is necessary for the protein to function in the desired environment.4. The designed protein should contain one or more Mur ligase N-terminal catalytic, Mur ligase C-terminal, Mur ligase central domains that are essential for its function.5. The protein design should take into account the physiological conditions of the Cell wall biogenesis, peptidoglycan biosynthesis pathway.6. The designed protein should be involved in cell wall organization, cell division, regulation of cell shape, peptidoglycan biosynthetic process, cell cycle by localizing to cytoplasm and exhibiting UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, ATP binding. The designed protein sequence is ",
    "response#1": "MKLTDILKKIPYKIVKGENDMVTGISMDSRTTEPGDVFICMRGVEVDGHRYAEDAVNRGAVVILAEKPVDVAVPVILVNDTSRALAMIAAAFYGHPSEKLKLIGVTGTNGKTTITYLLEEIFRIQKKRTGIIGTIQVNIDGKTFPVKNTTPDALTLQRYFHKMRKARVDQAIMEVSSHALDQGRVYGCDIDIAIFTNLTRDHLDYHENFDDYLRAKSLLFAQLGNGYNKEREKFAVINKDSPSAHRFIRSTSQGLLTYGIHEEADVMAEDYSLTETGTTFTMITPVGSIPINSPLMGMFSIYNMLAAAGAVSVAIGLEMEDVLKALETVSGAEGRLELVPAKRQFQVFVDYAHTPDALRNVLRTLRELEPHRLIAVFGCGGDRDRQKRPMMGDIVDQYADYAIITSDNPRTEDPNAILAEIEKGFRSSRYEKIVDRTEAIGRAVALAQPRDMVLIAGKGHETYQIIGDKTIHFDEREVVREALKDQS",
    "response#2": "MKEITGLALDSRKVQPGDLFLAVPGLRHDGRQHIEQAVAVGAVAVACETNDGFTCTASVPLLPIGHLVDQLSAECFTVTGTNGKTTTTFLIKHICEAAGLRCGLIGTVRYEIGERVLPALRTTPESLDLAELLAQIRDAGCRAAAMEVSSHALAQDRTRGLEWNVAVFTNLTQDHLDFHGTMASYFEAKAKLFAQLGAQQKKRTPVAIVNIDDRYGEQLLNKIDKNVRIITYGRSIRADFRASNPRVEFSGTSYQLDARGKSYLVRVPLIGTFNVSNSAAALATLLSLGYVFEDILITGTNGKTSVSQMLAQALNSLGQPCGVIGTLGSGMPGALFDHGMTTPDALAVQQQLAQLRDQGARRVSMEVSSHALDQGRVAALHFEVAVFTNLSRDHLDYHGDMPSYGAAKARILDQSHRAVINIDDAFGRELAERCTLPLLRYSLSDSSADLYCSDIRFDAEGISARLHTATATAELRSPLLGTFNLSNLLAVAGAGVAAGIPFAVIAAGLANCPGVPGRLELVPAKRQFQVFVDYAHTPDALRNVLRTLRELEPHRLIAVFGCGGDRDRQKRPMMGDIVDQYADYAIITSDNPRTEDPNAILAEIEKGFRSSRYEKIVDRTEAIGRAVALAQPRDMVLIAGKGHENYQIIGDKKHHFDDREVAAEALAAR",
    "response#3": "MKLSQLLAKLPHPILDADISSITSDSRLVQKGSLFIAYPGESSDGRKYIGDAIKKGAVAVIWDEQDFAWNEQWDVKNIAIANLKLQAGFIASQFYGQPSQSLKLVGITGTNGKTSVSQMLAQALNSLGQPCGVIGTLGSGMPGALFDHGMTTPDALAVQQQLAQLRDQGARRVSMEVSSHALDQGRVAALHFEVAVFTNLSRDHLDYHGDMPSYGAAKARILDQSHRAVINIDDAFGRELAERCTLPLLRYSLSDSSADLYCSDIRFDAEGISARLHTATATAELRSPLLGTFNLSNLLAVAGALYASGVPLDALAGAVPALKGVPGRFEQFHSADNIVGIVDYAHTPDALENVLETITHLRHGNEKVITVVGCGGNRDAEKRPKMAAIACKYSNLVILTSDNPRYEDPLDILEQMRKGVPAQDYKKTIVIPDRREAIFKAGLEANPEDIILIAGKGHENYQEIEGVRHPFDDLAVAQEALDRRN"
  },
  {
    "reference": "MPKPDKKAKLSDAEKLKSLKKKMKKLKAEAAEKEAAGIGPRVEAVTKAVVAKTTKKEKLVSPLAPASFPDLPVIAGVEFAAGAAGVKYKGRTDVMLVKLAAGTSIAGAFTKSSTRAASILDCQAKLAGKQETGQGAAIIVNSGNANAFTGVNGQAAVDKVTGGVASALSIPRDRVFSSSTGVIGEPLPADRITAIIGDLSRGMDAAGVADAAQAIMTTDTFPKGAAASFAGEGGEIRIVGIAKGSGMIAPDMATMLVYIFTDAAIAPEMLQKLLNAQLAATFNAITVDSDTSTSDALILAATGKSAAAPITDLRSATARRFAKALGQVMLDLAHQVVKDGEGATKFVEVQVTGAASAGDAHRVAMAIANSPLVKTAIAGEDANWGRVVMAVGKSGAQADRDKLTIRFGDMVLAENGWRAPSYDEAAASAYMKRDHLVVGVDLGMGRGKRTVWTCDLTHGYIDINADYRS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be involved in arginine biosynthetic process by localizing to cytoplasm and exhibiting acetyl-CoA:L-glutamate N-acetyltransferase activity, methione N-acyltransferase activity, glutamate N-acetyltransferase activity.2. A protein should be able to manipulate the activity of enzymes in the N(2)-acetyl- L-ornithine from L-glutamate: step 1/4, Amino-acid biosynthesis, L-arginine biosynthesis pathway, either by activating or inhibiting their function.3. The protein with the reaction acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate, the Nucleophile must be optimized to ensure efficient catalysis.4. The protein must exhibit the following characteristics: Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate..5. The protein should be able to bind substrate ligand in a variety of conditions6. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "MASSSALQGSAXXLVXGXAXRXHLXXGXGXGXLGXXAXAXRXFXXLXXRXGXIXDXVXSGXTAXGXI",
    "response#2": "MPAHEPHRPLHDLSALAARIEGGECSAXAXHXSAXGSXPXATXIXAXALLXAXMXAXAXXSAXPXAXAADAXAXDXXAXMAXAXAXASHXPXAXHXXFXXV",
    "response#3": "MSKAPVXXNXPPXEXPXDXRXLPXLAXLPPVXRXTAXDXMXLEXPXEXA"
  },
  {
    "reference": "MNALMRRACCGALFPLSFRLAALSPMKGASNFSCGNVCASPAGCWAPPSGHDTGIKVYNSLTRRKDPLILADPTVATWYSCGPTVYDHAHLGHACSYVRFDIIRRILLKVFGIDTVVVMVVTDIDDKIIKRAKELNISPVALARTYEQDFKQDMTALKVLPPTVYMRVTENIPQIISFIEHIIANGYAYATSQGNVYFDVQSIGERYGKFNDSFSDTASESASQDKRHIRDFALWKTSKPEEPYWASPWGKGRPGWHIECSTIASSVFGKHLDIHTGGIDLAFPHHENEIAQCEAYHQSTQWGNYFLHTGHLHLKGNEEKMSKSLRNYLTVKEFLKSFSPDQFRMFCLRSKYKSAVEYSNGSMHDAVNTLHTISSFVDDAKAYMKGQLICQPVQEALLWQRLNETKVNVKAAFSDDFDTPRAVDAVMDLIHHGNRQLKAVSKESNSPRSSVVYGAMISYIEQFLEILGISLSQNQVAAEDRHSAVLFNVVEEMISFRSKVRNYALAADESPNAIGQEEKQQYKERRRQLLLEREPLLQACDIMRQHLAVYGINVKDRGNTSTWELLDRKEET",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. Incorporating a 'KMSKS' region, 'HIGH' region motif is critical for the successful design of the protein. The designed protein sequence is ",
    "response#1": "LSIDGGGTTGIVAAASILHLEHQIRLQTGDPHAHISDFFDIVAGTGIGGILAALLVADDGSGRPMFTARDAVKFVAEKNSELFEIRYTGVFRRNKRYSGKSMERVLETAFRREDGKVLTMKDTCKPLLVPCYDLKTSAPFVFSRAGASESPSFDFELWKVCRATSATPSLFKPFSVVSVDGKTSCSAVDGGLVMNNPTAAAL",
    "response#2": "LSIDGGGTTGIVAAASILHLEHQIRLQTGDPHAHISDFFDIVAGTGIGGILAALLVADDGSGRPMFTARDAVKFVAEKNSELFEIRYTGVFRRNKRYSGKSMERVLETAFRREDGKVLTMKDTCKPLLVPCYDLKTSAPFVFSRAGASESPSFDFELWKVCRATSATPSLFKPFSVVSVDGKTSCSAVDGGLVMNNPTAAAL",
    "response#3": "LSIDGGGTTGIVAAASILHLEHQIRLQTGDPHAHISDFFDIVAGTGIGGILAALLVADDGSGRPMFTARDAVKFVAEKNSELFEIRYTGVFRRNKRYSGKSMERVLETAFRREDGKVLTMKDTCKPLLVPCYDLKTSAPFVFSRAGASESPSFDFELWKVCRATSATPSLFKPFSVVSVDGKTSCSAVDGGLVMNNPTAAK"
  },
  {
    "reference": "MKPVNPKLKPWKHPESQPRTACTQCYCKKCCYHCQVCFITKGLGISYGRKKRRQRRRPPEDSQNHQVPLPKQPSSQPRGDPTGPEESKKKVERETAAHPCD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit protein domain specific binding. The designed protein sequence is ",
    "response#1": "LPNRLVREAIEDGTVTELQGYGSIRPEVRYGEERSRIDLLLEGEPGRCWVEVKNVTLVEGERALFPDAVTERGQKHLRELMEMVRRGDRGVIFYVVQRGDGEAVSPADAIDPKYGQLLRLAVANGVEALAYRALVTPE",
    "response#2": "LPNRLVREAIEDGTVTELQGYGSIRPEVRYGEERSRIDLLLEGEPGRCWVEVKNVTLVEGERALFPDAVTERGQKHLRELMEMVRRGDRGVIFYVVQRGDGEAVSPADAIDPKYGQLLRLAVANGVEALAYRALVTPA",
    "response#3": "LPNRLVREAIEDGTVTELQGYGSIRPEVRYGEERSRIDLLLEGEPGRCWVEVKNVTLVEGERALFPDAVTERGQKHLRELMEMVRRGDRGVIFYVVQRGDGEAVSPADAIDPKYGQLLRLAVANGVEALAYRALVTPA"
  },
  {
    "reference": "MLNNNHIEIIKSTIPLLESAGPALTQHFYQRMFSHNPELKDIFNMTHQKTGRQSVALFEAVAAYAKNIENLGALATAVERIAHKHTSFNIKAEHYQIVGHHLIETLRELAPEAFTKEVEEAWTEAYLFLAQIFIDREEELYQLREKATGGWRGTRRFVVSEKRPESELVMSFVLTPEDGGKVLDYQVGQYLGIQVTPSKADNVEIRQYSLSQAPNGSHYRISVKKEGKGTEQQGLVSHYLHDEVEVGHAVDVLPPAGDFFYQESGKPVVLISAGVGCTPMQAMLQKLAEENKTDPVTYLHACENELQHSFIDEVETIVAAKGWQQNVWYRSPVNLDGSNNSSVGLMDLAQIKDTLPIHFGEFYTCGPVAFMENVVKQLEALGVGRDRIHYEVFGPHANL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The FAD, heme b binding site should be located in a region of the protein that is accessible to the ligand.2. The designed protein should have nitric oxide dioxygenase activity, oxygen binding, metal ion binding to facilitate response to toxic substance, response to nitrosative stress.3. For general function, the protein need meet that Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds; Therefore, plays a central role in the inducible response to nitrosative stress.4. The Charge relay system of the protein must be engineered to enable effective catalysis of NADPH + 2 nitric oxide + 2 O2 = H(+) + NADP(+) + 2 nitrate.5. The protein contains novel Globin family profile, FAD-binding FR-type domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MLSEKHISIVKSTVPLLESAGTAITEHFYKRMFAHNPELKNIFNMSHQHSGGQPAALFNAVAAYAKNIDNLGALSGAVERIAHKHTSFNIQPEHYPIVGHHLLETLRELAPDAFTPDVEEAWGAAYGVLAGIFIDPEDEEFYEGFKAFKVMKKVDESSLITSFYLQATDGSDVPAFQPGQYVTVRLTVSGEEYLLNRQYSLSDAPGKNYLRISVKREGAEPSTLGKVSNYLHDHVNEGEEIELTAPAGDFVLDDSRKPVVLISGGVGLTPMISMLNTLVEDNPNREVTFLHASVNRVHHAMKERVKELSQRHPKLRTYVCYDVPEAGDRDQFDHEGYIDLEWLRTVVPDNGAHYYFCGPLPFMKVIDKALKEWGVPDDRIHYEFFGPASSLEA",
    "response#2": "MLDSRTIEIVKSTVPLLESANTALTEHFYKRMFRDNPELKDVFNLSHQKTGRQPAALFAAVLAYAKNIDNPAVLSAAVERIAHKHTGFSIQPAQYDIVGHHLLETIKELAPDAATPEVIDAWAQAYGALAGIFIEQAEIYKRQAENPGGWEGFKAFKVMKKVDESSLITSFYLQATDGSDVPAFQPGQYVTVRLTVSGEEYLLNRQYSLSDAPGKNYLRISVKREGAEPSTLGKVSNYLHDHVNEGEEIELTAPAGDFVLQGARKFEVIEKTSESELVTSFVLAPEDGEVVIDFESGQYLGVSVAPINHDYQEMRQYSLSDKPNGKQYRISVKREAIGVPGVVSNYLHDHIHVGDSVDIYPPAGDFHYDAAENRPIVLLSGGAGITPMVSMLNTLAEQNPSRPVTFIHAAINGRVHALRNHVDALAAAHSNVRAHVCYEQPTQSDRQERDFDKEGYIDLAWMQQIVPSPASYYFCGPIPFMRMQHDALQALGVAEARIHYEFFGPAASLEPVA",
    "response#3": "MLSQQTIDVIQSTVPVLEVHGEAITRHFYQTLFTAHPELLNIFNHANQKQGRQQAALANMVYTAALHIDNLAAILPAVRQVAHKHRSLGIVPEQYAIVGTYLLQAIGDVLGEAATDEILAAWGDAYQVIADAFIGVEEQLYAQGARKFEVIEKTSESELVTSFVLAPEDGEVVIDFESGQYLGVSVAPINHDYQEMRQYSLSDKPNGKQYRISVKREAIGVPGVVSNYLHDHIHVGDSVDIYPPAGDFHYQAPDAPLVLLSGGAGITPMVSMLNTLVEKQPARRVVFVHAAVNGRVHAMRDHVQALAAANACFERWVCYNQPTEQDRHADRFHNEGYLDLAAVAALLPAADASFYFCGPTPFMAIVNAALERRGVPRERIHYEFFGPASALEVA"
  },
  {
    "reference": "MSSPDAGYVSDDQSQPRSAQPAVMAGLGPCPWAESLSSLGDVKVKGEVVASSGAPAGTSSRAKAESRIRRPMNAFMVWAKDERKRLAQQNPDLHNAELSKMLGKSWKALTLAEKRPFVEEAERLRVQHMQDHPNYKYRPRRRKQVKRMKRVEGGFLHALAEPQAGALGP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be able to modulate inner cell mass cellular morphogenesis in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "REMWMTHPQPLLPSRSPSALTPSVGSASGGKVLKTREGVVALGEVPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKVTVDGPREPRRR",
    "response#2": "PMMPKSWFFDTQSVSCDPENSDIVTLQTPLGMAKFIVIENSGCASLCMMVDVEPLALSSTKEIRFCDTIKVMNNRITAYEC",
    "response#3": "APPTLETCYFLNATILFLFRELRDTALARRWVTKKIKVEFEELLQTKTAGRLLEGLSLRDVFLGDTVPFIKTIRLVRPVVASGTGEPDDPDGDALPATCPEELAFEAEVEYNGGFHLAIDVDLVFGKSAYLFVKLSRVVGRLRFVLTRVPFTHWFFSFVEDPLIDFEVRSQFEGRPMPQLTSIIVNQLKKIIKRKHTLPSYKIRFKN"
  },
  {
    "reference": "MAGASAKVVAMLLSLQGPXSLRMVSESG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein have Expansin-like CBD domains with specific sequence motifs that are crucial for its activity.2. The protein must exhibit the following characteristics: May cause loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans..3. The protein design should be able to enhance the efficiency of cell wall organization. The designed protein sequence is ",
    "response#1": "LLKYVAGDGDVVEVEIKEKGSEEWKALKESWGAIWRIDTPKPLKGPFSVRVTTEGGEKIIAEDAIN",
    "response#2": "MRFTINGRNYFELVLISNVAGGGEISKVWIKGSKSNKWETMSRNWGANYQSNTYLNGQSLSFKVQLSDGSIKAALNVVA",
    "response#3": "TAALSTALFNDGKSCGGCYQILCDATKVPQWCLKGKSITITATNFCPPNFAQASDNGGWCNPPRPHFDMAQPAFLTIAKYKAGIVPILF"
  },
  {
    "reference": "MAKNLILWIVIAIVLMSIFNNFTATQTAPRTLAYSDFIDQVREGQVKEVTVQGKTIEGVGENGQRFTTYSPGDDGMVGDLLNNNVEIKAAPPEKPSMLMQILINWFPLFILIGLWIFFMRQMQGGAGGRGAMSFAKSRARMLSEDQVKVTFADMAGAEEAKEEVVEVVDFLRDPSKFQKLGGKIPKGVLMVGPPGTGKTLLARAIAGEAKVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFEGNEGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDVRGREQILKVHMRKVPTAEDVIASVIARGTPGFSGADLANLVNEAALFAARSNKRLVDMEDLEKAKDKIMMGTERRSMVMSDDEKKLTAYHESGHAIVGRLVPQHDPVHKVSIIPRGRALGVTLFLPEDDRFSYSKQRLESSISSLFGGRIAEELIFGLDMVTTGASNDIQRATEIARNMVTRWGLSDKLGPLTYSEDEQEVFLGHSVTQHKSVSDETSHLIDKEVRNVIDRNFERAKTILTEHMDELHAMANALIKYETIDADQIQDIMEGKEPRPPKQWTSDISPPGAGGETPTPGGDGKTAGGKSAGGPIGGPASEH",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The designed protein should have ATP binding, metalloendopeptidase activity, ATP hydrolysis activity, zinc ion binding, ATP-dependent peptidase activity within plasma membrane to regulate proteolysis, protein catabolic process.3. The Zn(2+), ATP binding site should be located in a region of the protein that is accessible to the ligand.4. The presence of AAA+ ATPase domains is critical for the interaction of the designed protein with its intended targets.5. The designed protein must have a active site that is structurally stable and resistant to denaturation.6. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins The designed protein sequence is ",
    "response#1": "MVMNEKEKVNTAYHEAGHAIVGRLMPEHDPVHKVTIIPRGRALGVTQFLPEEDKYSLSRRAIVSQICSLFGGRIAEEMTLGAEGITTGASNDIERATQLARNMVTKWGLSEKLGPLHYGEDESGQPGQGNPVSGKTSAEIDAEVRRIIDDCYSRAKKILEENRDILEAMKDALMEYETLDAEQVDAVNQ",
    "response#2": "IPKGALLEGPPGTGKTLLARAIAGEAGVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIVFIDEIDAVGRHRGAGYGGGNDEREQTLNQLLVEMDGFEANEGVIIIAATNRKDVLDPALLRPGRFDRQITVGNPDRPFDVDPAILARGTPGMAGADLANLVNEAALFAARGNKRLVDMDDFLASVDTAIARYRQSQQS",
    "response#3": "MVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLPEGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIVELSGCDDRVYDVLLVGAPGTGKTLLAKAVAGEANVPFFSMSGSEFVEMIVGVGAARVRDLFRQAREHAPSIVFIDEIDSIGRARGQVVLSGASEQEQTLNQILTEMDGFSGKEGVIVLAATNQPDILDKALLRAGRFDRRIVLHYVSSASGIEELLGLYVDIAEAIIR"
  },
  {
    "reference": "MNKDVLQGQSIIRYEEGQLIQTTDSYVTEFPLTINVNGTEFATVICSPTHMEELVVGFLASEGAIYKKEEIKSLQIDDSKGFAHVELTKDLGDRFEYSTKRMIASCCGKSREFYFHNDAAIAKTSMSKITLTPKQVLNMMTGLQTASTLFKQTGGLHNAAISDGDEFFEHRQDIGRHNALDKLYGFCLERRIPVRNKVLIFSGRISSEILLKAAKIGVGVILSKSAPTTLAITLANDLNITAIGFIRDGSFNIYSHPERIKATE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Required for formate dehydrogenase (FDH) activity2. The designed protein must be able to perform sulfurtransferase activity under specific conditions. The designed protein sequence is ",
    "response#1": "LAPLNKEYKIKRVVVSTYQSVTGTGVKAVEQLISEENNKDCQKVYPHPIHRNLFPHGGDFLENGYTTEEEKLVDETRKILSDKSIQLTATVVRVPVTGGHSESVNIEFENEFDIKDVISILSNTQGVVVMDNPKENIYPIPLLSEGKDDVFVGRIRRDLSQTKTLNLWIVSDNLRY",
    "response#2": "LSQTVLGLPFPHPIGLAAGLDKNGVAIPAWYRMGFSFVEIGTVTPRPQPGNPKPRLFRLIQDEALINRMGFNNEGAAAVARRLARVREASTGGILGINIGKNKDTPNAIAHEDYRAALEQVAPFADYLTINVSSPNTPGLRELQSEAFILDLLHAIEPIRSQYRLSIWVKLAPDLPNDEIERIVDALATSPFREHLGLICTNTTVQRPPLSGPNRDEAGGLSGRPLAARSTEVVRIAARAAQRRVPIMGSGGVFTAEDAYEKIRNGASLVQIYTALIYRGPAVVGELVRGLDALEW",
    "response#3": "LAPLNKEYKIKRVVVSTYQSVTGTGVKAVEQLISEENNKDCQKVYPHPIHRNLFPHGGDFLENGYTTEEEKLVDETRKILSDKSIQLTATVVRVPVTGGHSESVNIEFENEFDIKDVISILSNTQGVVVMDNPKENIYPIPLLSEGKDDVFVGRIRRDLSQTKTLNLWIVSDNLRR"
  },
  {
    "reference": "MLHKSPSRKRFASPLHLGCILTLTVLCLIAYYFALPDYLSVGKSSSRGAMDQKSDGTFRLKSIYRHGVGANHRLHQRLEVTPEVISAAGMLYQETTTQGQDFEDQEPLWTTNAEYATTNPFDFEFELRRMPLLMKRMKERDPEFIESYIYGETYMTEEEEHAMWIDDDIVAPNITDRGTVVSLALMSSNAYVRIPQTGDWRNVTEPWNETEPEDFGWDGDGIRGHVFYNEVENIVVLSIKGTSAQGLPGSGEDETTGNDKINDNLLFSCCCARVSYLWTTVCDCYVKSYICDESCLEKELRRKDRFYSAVVDIYKGVLKEYPDAAIWVTGHSLGGALASLLGRTFGLPAVAFESPGELLPSKRLHLPFPPGLPSYMEGIWHFGHNADPIFMGTCNGASSSCSLVGYAMETACHTGRVCVYDVVNDKGWSVNMFNHRIHKVIDEVLLGYEQAAKCVEPEPCVDCYNWKFIPSRDWESSSRLITKTKSHAAPTTTTRTTATTTSSSTCVGRNWLGFCTKYEL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The Lumenal topological domain of the protein should be designed to be flexible or rigid.2. The designed protein should have phospholipase activity within endoplasmic reticulum, multivesicular body membrane to regulate autophagy, phosphatidylserine catabolic process, macroautophagy.3. For general function, the protein need meet that The intravacuolar membrane disintegration by ATG15 is critical to life span extension.; Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles The designed protein sequence is ",
    "response#1": "VQFIVLFRDDDDNKDDSCKLLLYHDLTAHMLSTNEKEQKIGIIKGMWRFSNEFSQSNDYCHVELDSYSMLVLEVEPSYYLALGFENTHNSQWPKYKVRLSQCYQFFAMQHC",
    "response#2": "VQFIVLFRDDDDNKDDSCKLLLYHDLTAHMLSTNEKEQKIGIIKGMWRFSNEFSQSNDYCHVELDSYSMLVLEVEPSYYLALGFENTHNSQWPKYKVRLSQCYQFFAMQHC",
    "response#3": "VQFIVLFRDDDDNKDDSCKLLLYHDLTAHMLSTNEKEQKIGIIKGMWRFSNEFSQSNDYCHVELDSYSMLVLEVEPSYYLALGFENTHNSQWPKYKVRLSQCYQFFAMQHL"
  },
  {
    "reference": "MKVIKKLNLLNELTMLVCIFFFVCTISLIGIGIMYDLINRTSLSAPRRDPIFRNLNTVLIVLGVLEILLMVAQLVMSNMAANIINEVAENVEQKFAKALKWSRFLPFGLLQLYCYHKIKLVTQTDNI",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "MW",
    "response#2": "MTF",
    "response#3": "MSGKR"
  },
  {
    "reference": "MPEITVRAKSKTYPVYINEFALEDVREKWTESLAKFSHVFVLTDEHVAELHKAKLDAVLADLPVVTYYVAPNGEEAKTFRVYEDVMTKLIETGLDRKAVLIAFGGGVIGDLGGFVAATYMRGIPFYQVPTTVLAHDSAVGGKVAINHPLGKNMIGNFYQPEAVIYDTQFFATLPEREMRSGFAEMIKHALISDQTLLRALMDTFTEPKDFYTKDLTPFLQRGIEIKANIVAQDETEQGVRAYLNFGHTFGHALEAYGNFGKWLHGEAITYGMIYALTMSETIYGLDFDLAEFKTWLKQLGYDTTFDATVPFNKILENMRHDKKTTFNEISMVLLEEIGKPVIFKAEDDLIFETYKRVMRNGGNGI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should take into account the physiological conditions of the chorismate biosynthesis pathway. The designed protein sequence is ",
    "response#1": "LSETLKEQSWFSTPRSAWEKVKAVRQVERPSASDYISHIFDFFVESHGDRAFSDDKAMIGGLAFLDGQPVTIIADEKGKDFKECQERNFGMPMPEGYRKALRLMKQAEKFNRPVISFVNTSGAFCGIEAEERGQGEAIARNLMEMSSLKVPVLCLMIGEGGSGGALATAVGNEVWMMENATYSILSPEGFASILWKDANRAKEASEVMNITAQDLKRLGVIEEIIPEFGGADHKTTEAIAGYMKEHIKDFLRR",
    "response#2": "LSETLKEQSWFSTPRSAWEKVKAVRQVERPSASDYISHIFDFFVESHGDRAFSDDKAMIGGLAFLDGQPVTIIADEKGKDFKECQERNFGMPMPEGYRKALRLMKQAEKFNRPVISFVNTSGAFCGIEAEERGQGEAIARNLMEMSSLKVPVLCLMIGEGGSGGALATAVGNEVWMMENATYSILSPEGFASILWKDANRAKEASEVMNITAQDLKRLGVIEEIIPEFGGADHKTTEAIAGYMKEHIKDFLRELG",
    "response#3": "LSETLKEQSWFSTPRSAWEKVKAVRQVERPSASDYISHIFDFFVESHGDRAFSDDKAMIGGLAFLDGQPVTIIADEKGKDFKECQERNFGMPMPEGYRKALRLMKQAEKFNRPVISFVNTSGAFCGIEAEERGQGEAIARNLMEMSSLKVPVLCLMIGEGGSGGALATAVGNEVWMMENATYSILSPEGFASILWKDANRAKEASEVMNITAQDLKRLGVIEEIIPEFGGADHKTTEAIAGYMKEHIKDFLRSNA"
  },
  {
    "reference": "MTAQHNIVVIGGGGAGLRAAIAIAETNPHLDVAIVSKVYPMRSHTVSAEGGAAAVTGDDDSLDEHAHDTVSGGDWLCDQDAVEAFVAEAPKELVQLEHWGCPWSRKPDGRVAVRPFGGMKKLRTWFAADKTGFHLLHTLFQRLLTYSDVMRYDEWFATTLLVDDGRVCGLVAIELATGRIETILADAVILCTGGCGRVFPFTTNANIKTGDGMALAFRAGAPLKDMEFVQYHPTGLPFTGILITEAARAEGGWLLNKDGYRYLQDYDLGKPTPEPRLRSMELGPRDRLSQAFVHEHNKGRTVDTPYGPVVYLDLRHLGADLIDAKLPFVRELCRDYQHIDPVVELVPVRPVVHYMMGGVHTDINGATTLPGLYAAGETACVSINGANRLGSNSLPELLVFGARAGRAAADYAARHQKSDRGPSSAVRAQARTEALRLERELSRHGQGGERIADIRADMQATLESAAGIYRDGPTLTKAVEEIRVLQERFATAGIDDHSRTFNTELTALLELSGMLDVALAIVESGLRREESRGAHQRTDFPNRDDEHFLAHTLVHRESDGTLRVGYLPVTITRWPPGERVYGR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must have a active site active site that is highly conserved among related enzymes.2. The designed protein should be expressed specifically in the plasma membrane The designed protein sequence is ",
    "response#1": "NGDGNCLMHAASQYMWGVQDVDLVLRKTLYSALKEVDTRNFKLRWQRETIKSQEFVETGLHYDTRNWEEEWENIIKMTSTETPVARSGLQYNALEEIHIFVLVNVLRRPIIVLADKMLRSLESGSSFSPLNVSGIYLPLHWPAEECYRYPIVLGYDSMHR",
    "response#2": "NGDGNCLMHAASQYMWGVQDVDLVLRKTLYSALKEVDTRNFKLRWQRETIKSQEFVETGLHYDTRNWEEEWENIIKMTSTETPVARSGLQYNALEEIHIFVLVNVLRRPIIVLADKMLRSLESGSSFSPLNVSGIYLPLHWPAEECYRYPIVLGYDSMHY",
    "response#3": "NGDGNCLMHAASQYMWGVQDVDLVLRKTLYSALKEVDTRNFKLRWQRETIKSQEFVETGLHYDTRNWEEEWENIIKMTSTETPVARSGLQYNALEEIHIFVLVNVLRRPIIVLADKMLRSLESGSSFSPLNVSGIYLPLHWPAEECYRYPIVLGYDSMHC"
  },
  {
    "reference": "MENNQKPNKNMGGNGPKMNMPKFNMNWIYFIAILALGLLYFTSGGTANSSVAKTASYSEFKTMVERGYAKKIVVNKPQSTLRMYVKSEHIRDVFHQGTQQTGVEPYLEVEFGSVDQVEQFVAKAKEDKVFTGDLSFDNRRENEFLSLILSNLIPILFIVGLWIFLMRRMGGGGGGMGGGVFSVGKSKAKMYEKGGELGITFKDVAGQAGAKQEIQEIVDFLKNPQKYTDLGGKIPKGALLVGPPGTGKTLLAKAVAGEAGVPFFSMSGSDFVEMFVGVGASRVRDLFQQAKQKSPCIIFIDEIDAVGRARSKNPAMGGNDERENTLNALLTEMDGFGTNSGVIILAATNRADMLDSALLRAGRFDRQIHVDLPDLNERKEVFKVHLKPVKIDESVDIDFLARQTPGFSGADIANVCNEAALIAARHNSQTVTKQDFLDAVDRIIGGLEKKTKVMTQAEKRSIAIHEAGHATISWFTEFANPLVKVSIVPRGQALGAAWYLPEERVLQTKEAMLDEMCSLLGGRAAEELFVGHISTGAMNDLERTTKQAYGMIAYAGMSDKLPNVCYYNNAEYQFQKPYSETTAKIMDDEVLRMINEQYERAKQILTEHKEGHAQLAQLLIDREVIFAEDVEKIFGKRPWTSRAEELLEAQMKADAERMAEERAKELEAEAAQKALEAKGDEVNQEEKEDKDE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be able to bind ATP, Zn(2+) ligand in a variety of conditions2. A protein that contributes to proteolysis, cell division by functioning in plasma membrane with ATP hydrolysis activity, ATP binding, metalloendopeptidase activity.3. The designed protein have AAA+ ATPase domains with specific sequence motifs that are crucial for its activity.4. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity.5. The designed protein must have a active site active site that is highly conserved among related enzymes.6. For general function, the protein need meet that Plays a role in the quality control of integral membrane proteins.; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins7. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "MVMTEDEKKLTAYHEGGHAICALHMVDSDPIHKATIIPRGRALGMVMRLPEGDRISMSRAKLEADLVVAMGGRVAEELIFGEEKVTTGASSDIKMATNMARKMVTEWGMSNKLGPLLYAEDQEEVFLGHSVARHKNLSDETARLVDEEIRRIVDTAYGKARDILSTRQDELERLAQGLLEYETLSGDEIRARLPLFRQAGLLRAD",
    "response#2": "MVMTEDEKKLTAYHEGGHAICALHMVDSDPIHKATIIPRGRALGMVMRLPEGDRISMSRAKLEADLVVAMGGRVAEELIFGEEKVTTGASSDIKMATNMARKMVTEWGMSNKLGPLLYAEDQEEVFLGHSVARHKNLSDETARLVDEEIRRIVDTAYGKARDILSTRQDELERLAQGLLEYETLSGDEIREARRRFEARRIPRGVLLYGPPGTGKTLLARAVAGEAGVPFFTISGSDFVEMFVGVGASRVRDLFEQAKQNSPCIIFVDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFGERTGVILIAATNRPDILDPALLRPGRFDRQIPVGAPVSAQKIAAGTPGMAGADLENLVNEAALLAARKNHDYVTTEDFEPLRADVEASLIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFD",
    "response#3": "MVMSEKEKLNTAYHESGHAIVGRLMPEHDPVYKVSIIPRGRALGVTMFLPEEDKYSHSKRYLDSSICSLFGGRIAEELTLGFEGVTTGASNDIERATSLARNMVTRWGLSEKLGPLQYDTDSDEPFLGRSAGQAQTVYSPETAQRIDEEVRNIIDTCYEKAKQILVDNRDKLDLMADALMKYETIDRFQIDDAWRNAMEP"
  },
  {
    "reference": "MGGKWSKSSIVGWPAVRERIRRTEPRRAEPAAEGVGAASQDLDKHGALTTSNTASNNADCAWLEAQEEEEEVGFPVKPQVPVRPITYKAAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYNTQGYFPDWQNYTPGPGTRYPITFGWCFKLVPVDPREVEEANEGENNSLLHPVSLHGMEDEDREVLKWEFDIRLAHTHMARQLHPEFYKNC",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for virion component, membrane-specific folding and stability, while maintaining GTP binding.2. Incorporating a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Dileucine internalization motif; necessary for CD4 internalization motif is critical for the successful design of the protein.3. For general function, the protein need meet that Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis The designed protein sequence is ",
    "response#1": "LCNAILV",
    "response#2": "LFQFE",
    "response#3": "GYKXGXFXRX"
  },
  {
    "reference": "MNARWLGRGLVYLLILAAVIAIIYSLLPSRNSQVQSVPLSALVEDVRARQVQEIIVDGSTVTWKVMASDQQFRTELEPGDSVRKVLQDNGIPVTEQPPIRTKEGNPWGGVLSIVVNLLPLLLIMGILFFFLRQAQGSSSQALNFGKSRARLISGSRPTVTFADVAGVDEAKEELKEVVEFLKYPERFASLGARIPKGVLLVGPPGTGKTLLAKAVAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRNSPCIVFIDEIDAVGRHRGAGLGGSHDEREQTLNQILVEMDGFDTSTNVIVIAATNRPDILDPALLRPGRFDRQVVLDRPDVKGRRAILEVHCRGKPLEGDVDLDVLARQTPGFSGADLANLVNEAALLAARRGKRRISMREFNEAIDRVIAGPERKSRVISQREREIIAYHEAGHALVAHALPNADPPYKISIVSRGMAGGFTRFLPEEDRHLYTKSQFKDLLAAMLGGLVAEEMVFGEFTTGPQDDLERATRLARQMVTQFGMSERLGPRTFGRKEELVFLGKEIAEQRNYSEKIAEEIDEEVRQIIDHAYQTARKVLHDNRAKLDQLVRVLLERETLEGEELIAVLNASPEEEPLPPTPPAPPRQAREPGQVEAPPQPKPIMQPKPGLAFEGGGPVASQLDDISL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Zn(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "PELPEVETVRRGLQPHLEGRVIARAEARRPDLRWPLPPDLVQVLTGARVVALRRRSKYILAELEDRGSLLLHLGMSGRMLIEGESQGDFHRDPAILPRHDHVVLWNDQGTRITFNDARRFI",
    "response#2": "GLTVTEYVISCYGARKGLVDTALRTADSGYLTRRLVDVAQDMIIRETDCGTKSGILLGPVKDEQKVVFSLQERLIGRVLAENIYSPKQKQYIAKKNQDISASLSDKICEIKRTNILVRSPLTCKSIRSVCQLCYGWSLAHGNLVDLGEAVGIIAAQSIGEPGTQLTMRTFHTGGVFTGNIAKQIRSSIDGKIIYSLTNTIPMRTRHGEIALITQSNINICIQGKNKEFNIEVPINTILLVNNGSMVRAKQLIAEVSAPVNAQSVEIASKYVASDFSGEIHFENLLLQEKKSAYGHKMNRAGQYGGLLWILSGQVYKFSLDVPLMFDTADYIHNGSCMAEHNIISQYGGILQTSENNLTIDNSNSYLYVLNSSMTIINAYLSHIKNARSEAYILTDDLNQKFFMQVESNNVIKNNQIIAKFR",
    "response#3": "FGAAEAFLDDATAERDELRERVATLRADRQSAADRVAEAEALLDEGKCPECGQPVEGAPHVERVTDDRERVAELDAELADVEDELDAVAQRVDRGESLVK"
  },
  {
    "reference": "MRVRGIERNYQHLWRWGTMLLGILMICSAAGQLWVTVYYGVPVWKEATTTLFCASDAKAYKAEAHNIWATHACVPTDPNPQEIVLGNVTENFNMWKNDMVEQMHEDIISLWEQSLKPCVKLTPLCVTLNCTNAKIEQNVTVAGMRNCSFNMTTELKDKKKQEYALFYKLDVVQIDNSSTNTDYRLINCNTSAITQACPKITFEPIPIHYCAPAGYAILKCNNKTFNGMGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVIRSENLTNNAKIIIVQLNKSVEINCTRPSNNTRKGVHIGPGQAIYSTGQIIGDIRKAHCNISRKEWNSTLQQVTKKLGSLFNTTKIIFNASSGGDPEITTHSFNCNGEFFYCNTAGLFNSTWNRTNSEWINSKWTNKTEDVNITLQCRIKQIINTWQGVGKAMYAPPVSGIIRCSSNITGLLLTRDGGGADNNRQNETFRPGGGDMRDNWRSELYKYKVVRIEPLGIAPTKARRRVVEREKRAIGLGALFLGFLGTAGSPMGAVSMTLTVQARQVLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARILAVESYLKDQQLLGIWGCSGKHICTTNVPWNSSWSNKSLDYIWKNMTWMEWEKEIDNYTELIYSLIEVSQIQQEKNEQELLKLDSWASLWNWFSITKWLWYIKIFIMIVGGLIGLRIVFAVLSLVNRVRQGYSPLSFQTLLPAPRGPDRPEGTEGEGGEQGRDRSIRLLNGFSAIIWDDLRNLCLFSYHRLTDL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that localizes to viral envelope and can perform structural molecule activity with high specificity and efficiency.2. The protein should contain a Helical membrane-spanning region.3. The presence of Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains is critical for the interaction of the designed protein with its intended targets.4. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.5. For general function, the protein need meet that The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state The designed protein sequence is ",
    "response#1": "MRVMGIRKNYQHLWRWGTMLLGMLMICSAAEKLWVTVYYGVPAWRDADTTLFCASDAKAHSTEVHNIWATHACVPTDPNPQEIYLENVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTSANLTKVNNATTSNDFNPIIGNITDEVKNCSFNMTTELRDKKHKVYALFYSLDIVQIDNSSEYRLIKCNTSVIKQACPKISFDPIPIHYCTPAGYAILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENITNNVKTIIVHLNKSVEINCTRPSNNTRTSIAIGPGQLFYRTGDIIGDIRAAYCEINGTKWNKVLKQVTEKLKEHFKEKNITFQQPSGGDLEVITHHFNCRGEFFYCNTTRLFNNTCINNETIEGCQNITIPCKIKQIINMWQETGQAMYAPPIRGKISCISNITGILLTRDGGANKTNEEIFRPGGGNIRDNWRSELYKYKVVQIEPLGVAPTRAKRRVVEREKRAVGIGALFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQRLLGLWGCSGKIICTTAVPWNSTWSNKSYDEIWGNMTWIEWEREIGNYSSQIYDILTESQNQQDKNEKELLELDKWASLWNWFDITNWLWYIRIFIMIVGGLIGLRIVFAVLSVVNRVRQGYSPLSFQTRLPTPRGPDRPEGIEEEGGERDRD",
    "response#2": "LWVTVYYGVPVWKEANTTLFCASDAKAYDTEVHNVWATHACVPTDPDPQEVVLVNVTENFNMWKNNMVEQMQEDIISLWDQSLKPCVKLTPLCVTLNCADLENRTNSGNNNNSKSNITTDGSWKGEMKNCSFNITSSIRDKMQKEYALFYKYDIISIENNSTKYRLTSCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCKDTKFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVVIRSENITNNAKIIIVQLNESVTIKCVRPNNNTRKSINIGPGRAFYTTGEIIGDIRQAHCNISITKWNNTLKQIVDKLREQLGKNKTIIFNQSSGGDPEIVMHSFNCGGEFFYCNTTKLFNSTWNLTSAEGLNDTEGNDTLTLPCRIKQIINMWQEVGKAMYAPPIRGQIRCSSNITGLLLTRDGGRETEDTETFRPGGGDMRDNWRSELYKYKVVKIKPLGIAPTRAKRRVVQREKRXLGGLGAVFLGFLGAAGSTMGAASLTLTVQARRLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQLMGIWGCSGKLICTTAVPWNTSWSNKTLNQIWDNMTWMEWEREINNYTGLIYNLIEESQNQQEKNEQELLELDKWASLWTWFDISNWLWYIRIFIIIVGGLIGLRIVFAVLSIVNRVRQGYSPLSLQTRLPAQRGPDRPEGIEEEGGERDRDRSVRLVDGFLAIIWDDLRSLCL",
    "response#3": "MRVKGIRKNCQHLWRWGTMLLGMLMICSAAEKLWVTVYYGVPVWKEAKTTLFCASDAKAYDKEVHNVWATHACVPTDPSPQEMFLANVTENFNMWENDMVDQMHQDIISLWDESLKPCVKLTPLCVTLNCRNANLNSTRKSSLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVELKNVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNLRNDTANSNSTANDNSTNWEWIDKEMTNCSFNVTTIIRNKMKKEYALFYRHDVVQIGTDNTSYTLTHCNTSVITQACPKVSFEPIPIHYCTPAGFALLKCNDKKFNGKGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRTENSTNNAKTIIVQLNQTVEINCTRPNNNTRKSIHMGPGRWSVLYATGAIVGDIRQAHCNLSRQDWEKTLKKIVIKLKEQFNITKNITFKESSGGDPEITMHSFNCGGEFFYCNTTKLFNSTWYTNGNKTEESNNNDTITLPCRIKQIINRWQEVGKAMYAPPIQGRIYCSSNITGLLLIRDGGNSTDNESEIFRPGGGDMRDNWRSELYKYKVVQIEPLGVAPTKAKRRVVQREKRAVTLGAMFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVESYLRDQQLLGIWGCSGKLICTTNVPWNTSWSNKSLGEIWDNMTWIQWEREIYNYTGLIYTLLEESHYQQEKNEKELLELDQWASLWNWFDITRWLWYIKIFIMIVGGLVGLRIIFTVLSIVNRVRKGYSPLSLQ"
  },
  {
    "reference": "MGEGEENGNEADSNERSLALSPTWSVAIVLTVFVVVSLIVERSIYRLSTWLRKTKRKPMFAALEKMKEELMLLGFISLLLTATSSTIANICVPSSFYNDRFLPCTRSEIQEELESGSTVKRNLLTKSLFFNIFRRRLDVIKRTTCSEGHEPFVSYEGLEQLHRFIFIMAVTHVTYSCLTMLLAIVKIHSWRIWEDVARLDRHDCLTAVAREKIFRRQTTFVQYHTSAPLAKNRILIWVTCFFRQFGRSVDRSDYLTLRKGFIVNHHLTLKYDFHSYMIRSMEEEFQRIVGVSGPLWGFVVAFMLFNIKGSNLYFWIAIIPVTLVLLVGAKLQHVIATLALENAGLTEYPSGVKLRPRDELFWFNKPELLLSLIHFILFQNSFELASFFWFWWQFGYSSCFLKNHYLVYFRLLLGFAGQFLCSYSTLPLYALVTQMGTNYKAALIPQRIRETIRGWGKATRRKRRHGLYGDDSTVRTETSTIASLEEYDHQVLDVTETSFEQQRKQQEQGTTELELQPIQPRNDCVPNDTSSRVGTPLLRPWLSISSPTTTIELRSEPMETLSRSSSLPSEKRV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be involved in defense response, response to biotic stimulus by localizing to membrane and exhibiting calmodulin binding.2. The designed protein should have at least Helical; Name=5, Helical; Name=6, Helical; Name=7, Helical; Name=4, Helical; Name=3, Helical; Name=1 transmembrane domains to ensure proper localization.3. The protein must exhibit the following characteristics: Activity seems to be regulated by Ca(2+)-dependent calmodulin binding and seems not to require heterotrimeric G proteins .; May be involved in modulation of pathogen defense and leaf cell death.4. The Extracellular, Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells. The designed protein sequence is ",
    "response#1": "LFRGMDDLILDNICDRAKPRVFSKDEKIIREGDPVQRMIFIMRGRVKRIQSLSKGVLATSTLEPGGYLGDELLSWCLRRPFLDRLPPSSATFVCLENIEAFSLGSEDLRYF",
    "response#2": "MPLVELLTSAALGLSLQLLHDAIIRAKERSLITRCILDRLDATLHKITPFVIKIDTLTEEVDEPFRKVIEELKRLLEKAIRLVDAYAELKLRNLLRKYRYKRRIKELDSSLRWMIDVDVQVNQWLDIKKLMGKMSEMNTKLDDITRQPMDIIV",
    "response#3": "MPLVELLTSAALGLSLQLLHDAIIRAKERSLITRCILDRLDATLHKITPFVIKIDTLTEEVDEPFRKVIEELKRLLEKAIRLVDAYAELKLRNLLRKYRYKRRIKELDSSLRWMIDVDVQVNQWLDIKKLMGKMSEMNTKLDDITRQPMDIIG"
  },
  {
    "reference": "HGLPAQCPNADGTMVHTCCLHGMPTFKLNFDSHFTIKTVVAQNGTELPESILPEATIDRIPPSSHDLESVRGNLVRKNVDRLSLQEVNSLVHALKRMQKDRSSDGFESIACFHALPPLCPNPTAKHRYACCLHGMATFPQWHRLYVVQFEQSLNRHGATVGVPYTDWTYPMKEVPHLLTSEKYTDPFTAVETFNPFNHGHLSLLSPET",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be optimized for oxygen carrier activity. The designed protein sequence is ",
    "response#1": "PYTRDELEFNGVSIDSIAIEGTLETFFENFEYSLLNAVDDTVDIADVEILTYIERLNHKKFSFLILVTNNNNTEVLATVRIFAWPLRDNNGIEYSFNEGRWRALELDRFWVKVKHGHHQITRQSTESSVTVPDVPSLQTLIDRADAAISSGCALHLEDYESALGLPNRFLLPKGQAQGMEFNLVVAVTDGRTDAALDDLHENTKFIHYGYDRQYPDKRPHGYPLDRRVDDERIFEALPNFKQRTVKLI",
    "response#2": "PYTRDELEFNGVSIDSIAIEGTLETFFENFEYSLLNAVDDTVDIADVEILTYIERLNHKKFSFLILVTNNNNTEVLATVRIFAWPLRDNNGIEYSFNEGRWRALELDRFWVKVKHGHHQITRQSTESSVTVPDVPSLQTLIDRADAAISSGCALHLEDYESALGLPNRFLLPKGQAQGMEFNLVVAVTDGRTDAALDDLHENTKFIHYGYDRQYPDKRPHGYPLDRRVDDERIFEALPNFKQRTVKLS",
    "response#3": "PLYEVTPHMFTNSEVIHEAYKAQMTNTPSKFESHFTGSKKNPEQHVAYFGEDVGMNTHHVLWHMEFPFWWEDSSGRHLDRKGESFFWVHHQLTVRYDAERLSNHLDPVEELSWNKAIDEGFAPHTAYKYGGYFPSRPDNVHFSDVDGVARVRDMSMTEDRIRDAIAHGYIDALDGSHIDIMNSHGIEFLGDIIESSGYSANPGFYGSLHNTAHIMLGRQGDPTGKFDLPPGVLEHFETSTRDPSFFRLHKYMDNIC"
  },
  {
    "reference": "MTSLNTKSGTPVIPLLLRSDDASHTDTLVEEVSCSLGLGRDRIENILPSTAFQQDVIDCAGSEKQRSIGHVAYEISNDIDISKLAAAWKDTIHRTPALRTCAFTSSSGETYQVILKDSFVFSWMFSTSADQKDAVTKDEAAAAAYGPRCNRFVLLDDPIEKRKLLIWTFSHALVDTSFQERILERVLKAYTHGHDEVPNRPYTPESSDPEDDDLSLTPTDGSKTPETEGLHPATQYWKNYLSDLNASAFPHLTSPLAVPYPNAKSEHRFTFTASAQSTWPSLAICRTALAILLSRYTHSQEALFGVVTEQHQLLVNGPTRTVVPFRVHCASDQSLSDIIGAVYANDDAIRQFSDVGLRSISSTGDDGVAACGFQTVLSVTEGDNEQSSSCEILQKTGESELFMPCTNRALLLHCQMASDGLSMIARYDKSLIDSQQIARLLRQLGQLIQRLRVSPDELPSAGELDILTSEDQAEIQSWNSHPIPSQPTLIHKEMLKAASLSPSKVAICAWDGEWTYSELDNITSRLAALIRFSTPDQEHAILPIYFEKSKWVVASMLAVMKAGHAFTLIDPNDPPARVAQVVGQTGATVALTSKLYRKKVQGIIERCIIVDDDLVQSLICTCALKPDPTLAKVAPEDLAYVIFTSGSTGDPKGIMIEHRAFSSCALKFGSALGINSDTRALQFGSHAFGACLLEIMTTLIHGGCVCIPSDDDRMNNVPAFVNRANVNWMMATPSYMGTFQPDDVPGLKTLVLVGEQMSPSVNAIWAPR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be designed to catalyze the following reaction 3 (R)-2-hydroxy-3-methylbutyrate + 6 ATP + 3 L-phenylalanine +   3 S-adenosyl-L-methionine = 6 AMP + beauvericin + 6 diphosphate + 6   H(+) + 3 S-adenosyl-L-homocysteine.2. For general function, the protein need meet that Beauvericin nonribosomal cyclodepsipeptide synthetase; part of the gene cluster that mediates the biosynthesis of beauvericin (BEA), a non-ribosomal cyclic hexadepsipeptide that shows antibiotic, antifungal, insecticidal, and cancer cell antiproliferative and antihaptotactic activity; Ketoisovalerate reductase BEA2 catalyzes the NADPH-specific reduction of ketoisovaleric acid to hydroxyisovalerate, a precursor for beauvericin biosynthesis; The nonribosomal cyclodepsipeptide synthetase BEA1 then catalyzes the formation of beauvericin via condensation and cyclization of 3 dipeptidol monomers, each composed of one unit of hydroxyisovalerate and one unit of N-methyl-phenylalanine .3. The presence of Carrier domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "GHVYTMAMLRTRVESLARGLAADLNWSPNTGSPEEKVVAIYSLNTVALSGPLLTIMYKIDYFILCWAVHRLNGICMPLHSTCTSAEITSHMITASCTTIFTCSGLMSTCLEAAKELQLPAEKIYTLALPSTYLDNANLEDSPRLKTLEQLADQGSQLPQLEPLRWSAGQGKSQVAFLCSTSGTSGRQKLAMLTHYGIITNLLQMSAFEGFANDTYGQTVAAAIPFSHSYGILIGHVGVLRGESHIVFPRFDMQRMLGSVASYRVNRLYLVPPILAVLGANSFLMEPFDLSSVTSVVTGAAALDRTVAGRLKSLQPSWEFLHAWGLTETCIVVTFTSKHDVWYGSSGSLLPGCQLRLVDAEGKDVENYDQSGEVYYKAPNMFVGYFQDIFPCSRTQEVFLISQHANPEMYQCVVVAEIKCMKPGLSLNDDRLQNAWICVAQRHPALRTVFIESIQRPGHFDQVIMKHEAVPLECQLSSAQCEESAEGMFSRRVSISKEKGTTGRATILRRSENSAVFRLEVSHALMDGQSFGIIFHDFAQAYLQNELPSPTAFSYDSFVAYQEEIDRESVRAYWSSHLAGAQPTRFPTNGNRKLEDLKTLRFRIELDAAALQKVCLEYDATSANLCQVAWAVVLGSYTGSKDVCFSYVNSGRHAPLSSIENAVGAFVDVMVCRMKLPETAKISQMLSKAKQDVIQGLSHPGSLLFEEKKHGHGVSDLRGNTIITFQMGVRDEESAGSDLQIVMLDEITASDYDISLNIQPCHGGLDIRLDYWLSRIDQEIIESVAESFQTALACMCSDDIPLRDVNVVCTQEIE",
    "response#2": "LNTLVDKGLRRELPTREEALAVLATSDDELLDVVAAAGKVRRQWFGRRVKLNYLVNLKSGLCPEDCSYCSQRLGSKAGILKYTWLKPEEASKAAAAGVAGGAKRVCLVASGRGPTDRDVDRVTKTIEAIKEENEGIEVCACLGLLSDGQADRLRTAGADAYNHNLNTSEETYGAITTTHTYADRVETVQQAQAAGLSACSGLIAGMGESDKDLVDVVFALRDLDPDSVPVNFLIPFEGTPLAKEWNLTPQRCLRILAMVRFVCPDVEVRLAGGREVHLRSMQPLALHLVNSIFLGDYLTSEGQAGQADLDMIADAGFEVGWRQPAEETRPYVPLPVNFLIPFEGTPLAKEWNLTPQRCLRILAMVRFVCPDVEVRLAGGREVHLRSMQPLALHLVNSIFLGDYLTSEGQAGQADLDMIADAGFELCPEDCSYCSQRLGSKAGILKYTWLKPEEASKAAAAGVAGGAKRVCLVASGRGPTDRDVDRVTKTIEAIKEENEGIEVCACLGLLSDGQADRLRTAGADAYNHNLNTSEETYGAITTTHTYADRVETVQQAQAAGLSACSGLIAGMGESDKDLVDVGRIAAGFGGPVNFLIPFEGTPLAKEWNLTPQRCLRILAMVRFVCPDVEVRLAGGREVHLRSMQPLALHLVNSIFLGDYLTSEGQAGQADLDMIADAGFEVVEGS",
    "response#3": "KRAAESPSDPAVLSWDGAWTYSELDNVSSRLAAHIRSLDLSHEQLIVPVYFEKSKWVVASILAVLKAGHAFTLIDPKDPPARTTRIVQQTSAKVALTSKLHQDTVQAIIGRCIVVDDDFVQSLGSASQCQEKSELTVKPHNLAYAIFTSGSTGDPKGIMIEHQAFASCVAKFGPALIPHNARALQFASHGFGACLLEILPTLLRGGCVCIPSDLDRMHNIPDFIRRYNVNWMMATPSYMTTFKPEDVPGLQTLILVGEQMSASQEIVDMCAQVLGRPITRMRTSKSFVALGGDSLLAIKLMAHCREAGYTISIAKILQTASIEELSLLCPEDCSYCSQRLGSKAGILKYTWLKPEEASKAAAAGVAGGAKRVCLVASGRGPTDRDVDRVTKTIEAIKEENEGIEVCACLGLLSDGQADRLRTAGADAYNHNLNTSEETYGAITTTHTYADRVETVQQAQAAGLSACSGLIAGMGESDKDLVDVKNADDTPNKPAVCAWDGEWTYSELNNVSSRLASYISSLDLGQQLIVPIYLEKSKWVMAAILAVLKAGHAFTLIDPNDPPARTAQIIKQASASIALTSALHQSKMQTVVGRCITVDDDLFQTLTTFEGSQVASAAKPGDLAYVIFTSGSTGDPKGIMIEHRAFYSSVVKFGKALGIRSSTRALQFATHGFGAFLLEVLTTLIHGGCICIPSDHDRMHNIPGFIRQSQINWMMATPSYMTTMKPEDVPGLETLVLVGEQMSSSINDVWLSELQLLDGY"
  },
  {
    "reference": "MGYTVAVVGATGAVGTRMIQQLEQSNLPIDKVRLLSSSRSAGKVLQYKGQDVTVELTTEDSFTGVDIALFSAGGSVSAKFAPYAVKAGAVVVDNTSYFRQNPDVPLVVPEVNAHALEAHNGIVSCPNCSTIQMMVALEPIRQKWGLERIIVSTYQAVSGAGQSAINETQRELKEVLNDGKDPKDVQADILPAGGDKKHFPIAFNALAQIDVFTDNDYTYEEMKMTNETKKIMEDDSIAVSATCVRIPVLLAHSESVYIETKEVAPVDEVKVAIANFPGAVLEDDVVHQVYPQAVNAVGKRETFVGRIRKDLDAPKGLHMWVVSDNLLKGAAWNSVQIAETLHEKGLVRSTKELKFELK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that is capable of catalyzing reactions within the L-threonine biosynthesis, L-threonine from L-aspartate: step 2/5 pathway with high efficiency and specificity.2. A protein with NADP binding, protein dimerization activity, aspartate-semialdehyde dehydrogenase activity, NAD binding is needed to enhance diaminopimelate biosynthetic process in a specific context.3. The protein should be able to bind substrate ligand in a variety of conditions4. A key design requirement for the protein is the optimization of the Acyl-thioester intermediate to achieve high catalytic efficiency for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH. The designed protein sequence is ",
    "response#1": "MAIVGATGAVGQEMMKVLAERDFKIDELICLADPREEGTQLKFKDEVITVQKVTEEAFRKADIALFAVGTEISKELAPIAVKNNCVVIDNSYAFRLEEGVPLVVPEVNAEDIKEMPKGIIANPNCTTMAAMPVLKPLHDKAKILRVVVSTYQSVSGAGKQAMDELFAQTRAIFVSDSVETKNFPKRIAFNVIPEIDVFMEDGYTKEEWKMMAETKKILDPKIKLTATCVRVPVFIGHSEAVHIEFENPISADEARDILREAPGCLVIDKRERGGYITPHEAAGEDATYISRIREDATVENGLVLWCVADNLRKGAALNAVQIAELVALYQRDLVEWMSAMTYQAASGAGAQNMRELLTQMGEVHRVAKGLLDDPASAILDIDREVAGILREESFPTENFGVPLAGSLIPWIDKELENGQSREEWKGQAETNKILGREPNPVPIDGICVRIGAMRCHSQALTLKLRQDVPLDEIESMIAESSAWVKVVPNRRDSSMRELTPSAVTGTLTVPVGRLRKLNMGSHYLGAFTCGDQLLE",
    "response#2": "MAIVGATGAVGQEMMKVLAERDFKIDELICLADPREEGTQLKFKDEVITVQKVTEEAFRKADIALFAVGTEISKELAPIAVKNNCVVIDNSYAFRLEEGVPLVVPEVNAEDIKNAKNKLIANPNCSTIQMVVVLNPLHKEYGIRRLVVSTYQSVTGTGVKAVTQLMNEREGIGGEMAYKYPIDLNVIPQIDVFMDNGYTKEEMKMVNETKKIMRDDSIRVTATTVRIPVMGGHSESVNVEFNKDFDLTEIRSLLSEAPGVILVDDPANAQYPMPKDAHEKDDVFVGRLRRDESQPNTLNMWIVSDNLRKGAALNAVQIAEYLIDKDLV",
    "response#3": "MAIVGATGAVGQEMMKVLAERDFKIDELICLADPREEGTQLKFKDEVITVQKVTEEAFRKADIALFAVGTEISKELAPIAVKNNCVVIDNSYAFRLEEGVPLVVPEVNAEDIKDGILAKRGIIANPNCSTIQMVAALKPIYDAAGIERINVATYQSVSGSGKKGVDELAGQTVKLLNSQEAEPSTYKKQIAFNCLPHIDEFMDNGYTKEEMKMVWETQKILGDDSVAVNPTCVRVPVFYGHAEAVHIECQQPLDATEAMDLLSRAEGVEVYYGEDYPTQVKEATGKDHVMVGRVRNDISHPNGLNMWVVADNVRKGAALNSVQIAEILALYQRDLVEWMSAMTYQAASGAGAQNMRELLTQMGEVHRVAKGLLDDPASAILDIDREVAGILREESFPTENFGVPLAGSLIPWIDKELENGQSREEWKGQAETNKILGREPNPVPIDGICVRIGAMRCHSQALTLKLRQDVPLDEIESMIAESSAWVKVVPNRRDSSMRELTPSAVTGTLTVPVGRLRKLNMGSHYLGAFTCGDQLLG"
  },
  {
    "reference": "MVSLGGFARKLFGSANDRRVRGYKGRVDAINALEAEMKALSDEALAAKTAEFRRELADGKTLDDILVPAFAVVREAALRVLGLRPFDVQLIGGMILHERAIAEMKTGEGKTLVATLPVYLNALAGKGVHVVTVNDYLAQRDAGMMGRIYGFLGMTTGVIVHGLSDEQRRDAYACDVTYATNNELGFDYLRDNMKYERGQMVQRGHFFAIVDEVDSILVDEARTPLIISGPLDDRSDLYNTINEFIPLLSPEDYEIDEKQRSANFSEEGTEKLENMLREAGLLKGESLYDIENVAIVHHVNNALKAHKLFTRDKDYIVRNGEIVIIDEFTGRMMPGRRYSEGQHQALEAKEKVQIQPENQTLASITFQNYFRMYDKLAGMTGTAATEAEEFGNIYGLEVLEVPTNLPIKRIDEDDEVYRTVGEKFKAIIDEIKSAHERGQPMLVGTTSIEKSELLADMLKKSGFSKFQVLNARYHEQEAYIVAQAGVPGAVTIATNMAGRGTDIQLGGNPDMRIQQELADVEPGPEREAREKAIREEVQKLKEKALAAGGLYVLATERHESRRIDNQLRGRSGRQGDPGRSKFYLSLQDDLMRIFGSDRMDGMLQKLGLKEGEAIVHPWINKALERAQKKVEARNFDIRKNLLKYDDVLNDQRKVIFEQRIELMDAESVTDTVTDMRNEVIEEIVAKRIPERAYAEKWDAEGLKADVQQYFNLDLPIAEWVAEEGIAEDDIRERITAAVDKAAAERAERFGPEIMQYVERSVVLQTLDH",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.2. The protein should have a low Km value for ATP + H2O + cellular proteinSide 1 = ADP + phosphate +   cellular proteinSide 2..3. A protein that can perform protein-exporting ATPase activity, ATP binding in plasma membrane, cytoplasm to support protein import, protein targeting, intracellular protein transmembrane transport.4. For general function, the protein need meet that Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.5. The Zn(2+), ATP binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MGLFEKIFGTYSSREIKKIIPIINKIDSYEEEFKKLTDEELRNKTDEFKDMLAKGKTLDDILPEAFAVAREASARVLGMRHFREQLIGGIVLHQGRISEMKTGEGKTLVATLPAYLNALSGKGVHVITVNDYLAKRDRDQMSQLYGFLGLTTGVIVHDLDNEQRREAYNCDITYGTNNEFGFDYLRDNMVIYKEERVQRKLNFCIVDEVDSILIDEARTPLIISGEGDNSTDFYKVADFFAKTLKEDDYTVDEKTNSVILTEQGIEKAEKFFHIDNYGDGDNMQIQHHVVQALKANYTMKRDKDYMVKDNEVIIVDEFTGRLMEGRRYSDGLHQAIEAKENVKIQKESKTLATITFQNYFRMYTKLSGMTGTAQTEEAEFREIYGLDVIVIPTHRPIARIDAPDVVYKSEKAKFKAIVNEIAETYKKQQPVLVGTVSIEKSELLSDMLKRKGVPHQVLNAKYHEKEAEIISHAGEKGMVTIATNMAGRGTDIKLGEGVEEVGGLKVIGTERHESRRIDNQLRGRSGRQGDPGYSRFYVSLEDDLMRIFASDRLQGVVEKLGLTDEDAIPIEHKLISRAIENAQKRVEGQNFDIRKHLLEYDDVMNRQRQVIYEQRRNVLKGDELREDLLDMIEEVVEDFVPEYVDEKRHPDEWNLKGLEDRVLKQFSLRLDFSKSGDVGSLEDIQEKIVAAVNDLLNRKEAEFGKPLMDYLIRMISIQSIDSHWKDHLLAMDHLKEGIGLRGYGQKDPVREYQKEGYDLFMDMIRRVIQSKMFTRQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYAQRQEVIMEENDLSDVLMGMVKRTIGRVVDSHTQLEKEEWNLDGIVDFAASTLVHEDTISKKDLENKSAEEIKDYLVARAQEVFEEKSQQLNGQEQLLEFEKVVILRVVDTKWTDHIDAMDQLRQSVGLRAYGQNNPLVEYQTEGYSMYNNMVGS",
    "response#2": "MGLFEKIFGTYSSREIKKIIPIINKIDSYEEEFKKLTDEELRNKTDEFKDMLAKGKTLDDILPEAFAVAREASARVLGMRHFREQLIGGIVLHQGRISEMKTGEGKTLVATLPAYLNALSGKGVHVITVNDYLAKRDRDQMSQLYGFLGLTTGVIVHDLDNEQRREAYNCDITYGTNNEFGFDYLRDNMVIYKEERVQRKLNFCIVDEVDSILIDEARTPLIISGEGDNSTDFYKVADFFAKTLKEDDYTVDEKTNSVILTEQGIEKAEKFFHIDNYGDGDNMQIQHHVVQALKANYTMKRDKDYMVKDNEVIIVDEFTGRLMEGRRYSDGLHQAIEAKENVKIQKESKTLATITFQNYFRMYTKLSGMTGTAQTEEAEFREIYGLDVIVIPTHRPIARIDAPDVVYKSEKAKFKAIVNEIAETYKKQQPVLVGTVSIEKSELLSDMLKRKGVPHQVLNAKYHEKEAEIISHAGEKGMVTIATNMAGRGTDIKLGEGVEEVGGLKVIGTERHESRRIDNQLRGRSGRQGDPGYSRFYVSLEDDLMRIFASDRLQGVVEKLGLTDEDAIPIEHKLISRAIENAQKRVEGQNFDIRKHLLEYDDVMNRQRQVIYEQRRNVLKGDELREDLLDMIEEVVEDFVPEYVDEKRHPDEWNLKGLEDRVLKQFSLRLDFSKSGDVGSLEDIQEKIVAAVNDLLNRKEAEFGKPLMDYLIRMISIQSIDSHWKDHLLAMDHLKEGIGLRGYGQKDPVREYQKEGYDLFMDMIRRQAEDSSQLYLQINALIPRLKRHIEEEEGVVTQEGHYVVDEKTRQIELNEQGHQFIEELLASAGLLPEGDNLYSAHNLQLLTHVYAGLRAHVLFHRNVEYIVQGNQVLLIDEHTGRTMQGRRLSEGLHQAI",
    "response#3": "MGLFEKIFGTYSSREIKKIIPIINKIDSYEEEFKKLTDEELRNKTDEFKDMLAKGKTLDDILPEAFAVAREASARVLGMRHFREQLIGGIVLHQGRISEMKTGEGKTLVATLPAYLNALSGKGVHVITVNDYLAKRDRDQMSQLYGFLGLTTGVIVHDLDNEQRREAYNCDITYGTNNEFGFDYLRDNMVIYKEERVQRKLNFCIVDEVDSILIDEARTPLIISGEGDNSTDFYKVADFFAKTLKEDDYTVDEKTNSVILTEQGIEKAEKFFHIDNYGDGDNMQIQHHVVQALKANYTMKRDKDYMVKDNEVIIVDEFTGRLMEGRRYSDGLHQAIEAKENVKIQKESKTLATITFQNYFRMYTKLSGMTGTAQTEEAEFREIYGLDVIVIPTHRPIARIDAPDVVYKSEKAKFKAIVNEIAETYKKQQPVLVGTVSIEKSELLSDMLKRKGVPHQVLNAKYHEKEAEIISHAGEKGMVTIATNMAGRGTDIKLGEGVEEVGGLKVIGTERHESRRIDNQLRGRSGRQGDPGYSRFYVSLEDDLMRIFASDRLQGVVEKLGLTDEDAIPIEHKLISRAIENAQKRVEGQNFDIRKHLLEYDDVMNRQRQVIYEQRRNVLKGDELREDLLDMIEEVVEDFVPEYVDEKRHPDEWNLKGLEDRVLKQFSLRLDFSKSGDVGSLEDIQEKIVAAVNDLLNRKEAEFGKPLMDYLIRMISIQSIDSHWKDHLLAMDHLKEGIGLRGYGQKDPVREYQKEGYDLFMDMIRRPIEHKLISRAIENAQKRVEGQNFDIRKHLLEYDDVMNRQRQVIYEQRRNVLKGDELREDLLDMIEEVVEDFVPEYVDEKRHPDEWNLKGLEDRVLKQFSLRLDFSKSGDVGSLEDIQEKIVAAVNDLLNRKEAEFGKPLMDYLIRMISIQSIDSHWKDHLLAMDHLKEGIGLRGYGQKDPVREYQKEGYDLFMDMIRR"
  },
  {
    "reference": "MTISYDLKPIIVRKAFASAVPADLSAKTSLIGLTRDEMAKALQSIGVPVRQVHMRLSQLWHWLYVRGVSSFDDMFNISGELRNRLAGRFSITRPRIVAEQVSNDGTRKWLLRFPSCGAGRSVEVETVYIPQEGRGTLCVSSQVGCLLACSFCYTGTQRLVRNLTAEEILAQLLTARDCLRDFPDKNTPGGAIVPQERRKISNIVMMGMGEPLYNFDAVKKALLIASDGEGLSFSRRRITLSTSGVVPKIYRVGKEIGVMLAISLHAVDDELRNILVPLNKKYPLAELMEACRNYPGLGNACHITFEYVMLKDVNDSVKDARKLVALLKGISAKINLIPFNPWLGTNYQCSDWKQIEHFADIVNKAGYASPIRTPRGRDILAACGQLKSQSKPLRKMERLQLEVGV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity.2. The Proton acceptor, S-methylcysteine intermediate active site of the designed protein must be optimized for substrate binding and specificity.3. The presence of [4Fe-4S] cluster is necessary for the protein to function in the desired environment.4. For general function, the protein need meet that m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs5. The protein must have high specificity towards metal ion binding, rRNA binding. The designed protein sequence is ",
    "response#1": "VGRSGALCVSSQVGCTLNCTFCHTGTQALVRNLTAAEIVQQVLIAKDDLAEWPSRLEDRQITNVVFMGMGEPLYNLDHVADAIDVISDGEGIGIGRRRITVSTSGVVPKIEELGARTNTMLAISLHATNDALRDELVPLNKKYPIAELLDAIRAYPELSNSKRVTFEYVMLKGVNDSPGEAKALVRLLQGIPSKINLIPFNPWPGSPYECSDWATIERFADIVNKAGYASPIRH",
    "response#2": "EDGRGTLCVSSQAGCTLDCAFCSTGKQGFNRNLSAAEIIGQLWLANRALGIHAGGARAISNVVFMGMGEPMLNLDAVIPAARLMIDDNGYGLSRRRVTVSTSGVVPGMDRLAGECPVALAVSLHAADDALRDRLVPINRKYPIRQLLAACIRYLERAPRDFITFEYVMLDGVNDSDAQAKALLGIASQVRCKFNLIPFNPFPDSGFKRSSPERIRRFAEVLQRAGLTVTTRKTRGEGIDAACGQLRGRNTNSVLDHTSEELRQAFAREGLERFRADQVLRWVYGQRVRDFRLMSDLARPLRERLYETWSLGCLERESSFESTDGTRKLVLRTHDGAKIESVLIPEPRRQTLCVSSQVGCSLDCSFCATGRLGLGRNLRAGEIVDQVLHGAEVLARSGKEITHVVFMGMGEPLLNLDNVIRSIRILADPRGMGLSPRRVTVSTAGVVPRMAELGRALPVRLTVSLHATTDEVRNELVPLNRRFPIATLLEACRRYPASRRDRLSFEYTLIRGVNDSPDDARRLVKLLHGLRAKVNLIPMNEHPGSAYRRPEAPQIEAFAGTLAAARLPVTVRRSRGDDIFAACGQLGAPQSL",
    "response#3": "VEDRGAVCISSQVGCTLSCRFCHTGTQTLVRNLGAPEIVGQFMAARDSYGEWPSPKGETPRLLSTIVLMGMGEPLYNYTNVAKAMKIVMDGEGIGLSRRRITLSTSGVVPMMDRAGAELGVNLAVSLHAVTDELRNELVPLNRKYPIAEVLAACRRYPPASNARRITFEYIMLKGVNDSEAEARELVRLIAGLPAKVNLIPFNPWPGSKYEVSGKAALDRFAAIVMAAGFSAPIPRAKGGA"
  },
  {
    "reference": "MKKQCFFEFASVFASSMSFILSILREMLEYFGVPINQILHIWENKDYGSARSIVRIIGKLLPLEPCPRPNFELVPLLNSVDPANCGSVVPSFADVWCVANDEEASYLRFRTWKNEEEEKIASFHPLQLFEGPLTPAVRRNKPRKNDWPESETAIKKIAALEDELAFLRSQIAAIVGRQELGNSTKAGFLDLNGRPSGFGQKPSSGATQLNVKQDSFSSSVRPSSPPPPSPPQISSVQPPCSPLMKIASNSICASDNSTTEVKQQHPDAGKTNYSHHSKNQKNEAVPNMLDVLKDMNKVKLRAIERSPGGRPIHKRKREDSPWDPVSLISHALKQKFAFQEEDSFEKEDNCWESSPFSSPETSRVRL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Ensure the presence of a signal peptide within the protein.2. The protein should exhibit Polar residues compositional bias.3. The protein design should aim to mimic or enhance the function of natural proteins involved in aerobic respiration, mitochondrial fission, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "NILGRGENISLQGSYSSTRANDLQLKFWKPFFHTRFKENRPEMSFSIFRQTDRFDISSFQTTNIGYLVDFSAHTMVGVDLTHSLQYENAIRDVGLLNKSVPFAIRDHCGPKLASLLRYSVVYDNRDGNVFPTRGIYLKSVNEYCGLGGNVAYTSSTAHGELNVPLFAGLVAQFCARVGVVKETKNTTQLPISSLFYCGGPLTLRGFKFGGAGPVVESTPIGAQSFWCTGAHLWAPLPFAGVFKNLASHFRMHFFYNIGNNNSFSTENMRSAFGMGLAVKLAERARIELNYCVPVRHQDTDRILNGFQFGIGYEL",
    "response#2": "NILGRGENISLQGSYSSTRANDLQLKFWKPFFHTRFKENRPEMSFSIFRQTDRFDISSFQTTNIGYLVDFSAHTMVGVDLTHSLQYENAIRDVGLLNKSVPFAIRDHCGPKLASLLRYSVVYDNRDGNVFPTRGIYLKSVNEYCGLGGNVAYTSSTAHGELNVPLFAGLVAQFCARVGVVKETKNTTQLPISSLFYCGGPLTLRGFKFGGAGPVVESTPIGAQSFWCTGAHLWAPLPFAGVFKNLASHFRMHFFYNIGNNNSFSTENMRSAFGMGLAVKLAERARIELNYCVPVRHQDTDRILNGFQFGIGYEL",
    "response#3": "LYRNILMYGPPGTGKTLFAKKLAMHSSMDYAIMTGGDVAPMGREGVTAMHKVFDWAGTSKRGLLLFVDEADAFLRKRSTEKISEDLRATLNAFLYRTGEQSNKFMLVLASNQPEQFDWAINDRIDEIVHFDLPSREDRK"
  },
  {
    "reference": "MASLKNCNLLLIVTGGIAAYKTPELVRRLRDQGVSVRVVMTKGAQAFITPLTLQAVSGHPVHDDLLDPAAEAAMGHIELARWADLVVVAPASASAIARLAHGHADDLFSTLCLATDAPIAVVPAMNRLMWAAPAVQRNVELLQQDGKLMWGPAEGSQACGEVGAGRMIEPADIVANVENFFHTTAHLEPTGELAGQHVVITAGPTREAIDPVRYLSNHSSGKMGFALAEAAASAGAQVTLIAGPVQLDTPPGVDRIDIQSAQEMLEQVQRALPESDIFIGCAAVADFRAMEVADNKMKKSAEQNEMTITLTKNPDILAWVASQSNRPFTVGFAAETQNVEEYATRKLRAKNIDMIVANDVSKSGQGFNSDNNAVEVYWPLNDKEHGHEAFASRSKQNLAKDLIQLIYKKKQHG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A key design requirement for the protein is the optimization of the Proton donor to achieve high catalytic efficiency for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.2. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.3. The designed protein must possess FMN binding, phosphopantothenate--cysteine ligase activity to support pantothenate catabolic process, coenzyme A biosynthetic process under certain conditions.4. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A5. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.6. The designed protein should contain one or more Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains that are essential for its function.7. A protein that can modulate the overall flux of metabolites through the Cofactor biosynthesis, coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MRIVLGVTGGIAAYKAALVLRLLKEAGHRVRVVPTESALRMVGRPTWEALSGEPVRTSVFDDAEHVDHVALGRGADLVIVAPATANLLAKAATGLADDLLSTTLLTVTCPVLLAPAMHTEMWLHPATQANVATLRGRGVHVLDPDSGRLTGADSGPGRLPEPAAIVAAALAALDPRPLAGRRILVTSGPTHEPIDPVRYIANRSSGAQGSAIAAALARLGAELVFVTGPATAPRPEGVQCIEVETAREMRDAVMAALPVDAAIFAAAVADWRVENAVTSKMKKDATGAPPALRLAENPDILREVSRLEQNRPQLVVGFAAETDDVIANATAKRERKGCDWILANDVRPDTGIMGGSENAVTLLAADGSEAWPRMGKDEVARRLAERIAGALG",
    "response#2": "MRILLGVAGGIAAYKAVLLLRLLREAGHDVRVIPTHAALEFVGRPTWEALSGHPATPEVFEDVEHVPHVSLGQGADLLVVAPATADLLSRAATGRADDLLTSTLLTVRCPVLLAPAMHTEMWDHPATRANVATLRQRGVHVLEPASGRLTGSDTGPGRLPEPAEIAAAARLALAGPLAGRRVLISAGPTAEPIDPVRFITNRSSGKMGFSIARMAAQRGASVTLVAGPTNQSDPYGVTVERVKSSEDMHQTVFHHLDGQDVVIMAAAVADYRPKQTADHKIKKGAPTLILKMERTRDILKEIAQNKPPVLVGFAVETHEGLQYAKQKLIDKNLDLIVYNDITVEGAGFDVDTNIVTLLAADGTVVLAGRHVVVSAGGTREPIDPVRFLGNRSSGRQGLALARAARARGARVTLVAANVTLPDPDGVDVVHVESTAELRDAVHAAAKDADVVVMAAAVADFRPASTAGHKIKKRDDGVVAPIALEANPDVLAELAGELRRPGQVVVGFAAETGDDAGDVLEHGRHKARRKGADLLAVNAVGGGLGFGTADNEVTILDAHGAPVAEASGDKLALAHVVLDTVRELVG",
    "response#3": "MRILLGVAGGIAAYKAVLLLRLLREAGHDVRVIPTHAALEFVGRPTWEALSGHPATPEVFEDVEHVPHVSLGQGADLLVVAPATADLLSRAATGRADDLLTSTLLTVRCPVLLAPAMHTEMWDHPATRANVATLRQRGVHVLEPASGRLTGSDTGPGRLPEPAEIAAAAARLLAAPGSALAGRHVVVSAGGTREPIDPVRFLGNRSSGRQGLALARAARARGARVTLVAANVTLPDPDGVDVVHVESTAELRDAVHAAAKDADVVVMAAAVADFRPASTAGHKIKKRDDGVVAPIALEANPDVLAELAGELRRPGQVVVGFAAETGDDAGDVLEHGRHKARRKGADLLAVNAVGGGLGFGTADNEVTIVGADGRRTAVPRGSKSALAHAVWDAVVGR"
  },
  {
    "reference": "MEKKTDEVFDALFKLEEIRQILRDSLPTGLDNDEKEAWKSKLSELNEIVQKLDSESVTRPLKKIAGTIEIRSREENYINIQPIQAAGRLTLEARKAIIAYGDGYSTCDTCRKPFRLDKISKPPIGDFHTELARFVNMDQARVVPGARRGFQAVAQTMVQRGDSVIVSSFAHYTEFLAVEGAGGQVREVPVDKNNLITADATATKIEDVIRETGKNPALVMMDHIDYQVANEHDVTGIAKVAHQYDIPFLYNGAYTVGIMPVDGKAIGADFVVGSGHKSMASPAPSGMLATTDEWAPKVLRTTQMVGDLTSRKFGVKEVEMLGCTLMGSNLIAMMASFPEVQKRTQNWDDEVKKSNYFIDALLTIEGSRVLSEYPRKHALSKVDTTGSFDIVAKTHKRKGFYFSDELSARGIVGEFAGATRTWKLSTYGLSWKKVQYLADAFTEIAEKYELPLKSKT",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be able to modulate translation in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "ECW",
    "response#2": "MGKTNGKFRYNFHRRDWRG",
    "response#3": "MNTIDLPFL"
  },
  {
    "reference": "MSSYFVNPLFSKYKGGESLEPAYYDCRFPQSVGRSHALVYGPGGSAPGFQHASHHVQDFFHHGTSGISNSGYQQNPCSLSCHGDASKFYGYEALPRQSLYGAQQEASVVQYPDCKSSANTNSSEGQGHLNQNSSPSLMFPWMRPHAPGRRSGRQTYSRYQTLELEKEFLFNPYLTRKRRIEVSHALGLTERQVKIWFQNRRMKWKKENNKDKLPGARDEEKVEEEGNEEEEKEEEEKEENKD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should emphasize Basic and acidic residues, Polar residues compositional bias.2. Incorporation of the Homeobox domain in the designed protein should not interfere with its overall stability and folding.3. The protein should be able to modulate anterior/posterior pattern specification in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "GRKQDIGDILQQIMTITDQSLDEAQARKHALNCHRMKPALFNVLCEIKEKTELKEARSRNCPSLFFLVTRIQSSVRHRVLSSASTSGNPVTPQPTHHHPSFGWGEGKVLSIRGAQEEEPTDPQLMRLDNMLLAEGVAGPEKGGGSAAAAAAAAASGGAGSDNSVEHSDYRAKLSQIRQIYHTELEKYEQACNEFTTHVMNLLREQSRTRPISPKEIERMVSIIHRKFSSIQMQLKQSTCEAVMILRSRFLD",
    "response#2": "VLSIRGAQEEEPTDPQLMRLDNMLLAEGVAGPEKGGGSAAAAAAAAASGGAGSDNSVEHSDYRAKLSQIRQIYHTELEKYEQACNEFTTHVMNLLREQSRTRPISPKEIERMVSIIHRKFSSIQMQLKQSTCEAVMILRSRFLG",
    "response#3": "VLSIRGAQEEEPTDPQLMRLDNMLLAEGVAGPEKGGGSAAAAAAAAASGGAGSDNSVEHSDYRAKLSQIRQIYHTELEKYEQACNEFTTHVMNLLREQSRTRPISPKEIERMVSIIHRKFSSIQMQLKQSTCEAVMILRSRFLS"
  },
  {
    "reference": "MFRSHTCGELRLSDAGKSVTLAGWVQRARKMGGMTFVDLRDRYGITQLVFNEAVNAELCERANHLGREFVIQITGEVNERSNKNMNIPTGEIEIIVSVLNVLNSAVTPPFTIEDNSDGGDDIRMKFRYLDLRRNCVRKNLELRHKMTMEVRRYLDSKGFLEVETPMLVGSTPEGARDFVVPSRMNPGQFYALPQSPQTLKQLLMVSGFDRYFQIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDIISTFEGMAKHLFKELRGVELSEPFLRMTWADAIKYYGSDKPDLRFGMKFVELMDIMKGHGFSVFDDAAYIGGICAEGAASYTRKQLDQLTEFVKKPQIGAKGMVYARVEADGNVKSSVDKFYSQEVLQQMKEAFSAKPGDLILILSGPDAMKTRKQLCELRLEVGRQLGLRDKNKFACLWVVDFPMFEWSEEEGRLMAMHHPFTHPKEEDIPLLDTDPAAVRADAYDMVVNGVEVGGGSIRIHDSQLQAKMFEILGFTPERAQEQFGFLMNAFKYGAPPHGGLAYGLDRWVSLFAGLDSIRDCIAFPKNNSGRDVMLDAPAALDQSQLDELNLVVDIKEDK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be involved in aspartyl-tRNA aminoacylation by localizing to cytoplasm and exhibiting aspartate-tRNA ligase activity, nucleic acid binding. The designed protein sequence is ",
    "response#1": "KEEIRLKHRYLDLRRERMKKNLHLRHATIQTARNFLEEEGFIEVETPILTRSTPEGARDYLVPSRVCEGEWFALPQSPQIFKQLLMVGGIERYYQVARCFRDEDLRSDRQPEFTQLDMEMSFMSQEEILCLNERLIACIWKKIKGKDIKVPFPRLSWQESMDRYGTDRPDTRYGMELVDVSSIVKDIGFKVFSGAIQAGGSVKCIKVEEGNQSISNVRIKPGGDVFNEAQKAGAKGLAFIRVRVNNEIDTIGAIKDNLNNQQKNELLLKTKAKPGDLILFAAGDTEIVHKTLDKVRQFLAKELRLISTGKSKDQWNFLWVIDFPMFNFNKDEKRHEAMHHPFCAPNAKDIGGDPGLWEENLPKARAQAYDLVLNGLELGGGSLRIHNPELQQKVLETIGIAKDEATEQFGFLLNALEMGAPPHGGLAFGLDRIVMLLSEEDSIRDTIAFPKTR",
    "response#2": "LRHEVYQAVREFLAGNGFIEVETPMLTKSTPEGARDFLVPSRLEKGKFYALPQSPQLFKQILMVAGLERYFQIAKCFRDEDLRADRQPEFTQIDLEMSFVTEDDVMTLAESLIQYVYKKVLGIDIKIPFRRMSYEEAINKYGTDKPDLRYGLELIDITDLAKEVEFKVFNDVAKSGGLVKGINIKGGSKFSRKEIDELTEYAKKFGAKGMAWVKLENGEATSPILKFFTEEQKQKLFSLMDAKDGDLLIFIADKKDITHKVLGFLRKHIAEKMNLIDNSKLEFLWVVDFPLFEWDEEENRLVAIHHPFTSPKDEDIDRLDEALNSPEIALSFKSKAYDMVLNGEEIGGGSIRIHTPYIQEKVFQLLNISEEEAKEKFGFLIEALSYGAPPHGGLAFGLDRILALMTGSESIRDVIAFPK",
    "response#3": "FSGAIQAGGSVKCIKVEEGNQSISNVRIKPGGDVFNEAQKAGAKGLAFIRVRVNNEIDTIGAIKDNLNNQQKNELLLKTKAKPGDLILFAAGDTEIVHKTR"
  },
  {
    "reference": "MSAPRLVFATGNSHKISELEAILAPAWEGFDSGTIARMSDFDADAPVEDGASFEENALIKARHLAAFTGLAALADDSGLTVDVMGGAPGIFSARWCGRHGDDAANLDLLLAQLADVPDALRSAAFVSAAVLVLPDGREFVERGQVRGRLLRERRGGGGFGYDPVFVPDGHALTTAQMSAEQKNAISHRGQAFRALAPAIIDYLRS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate, Mg(2+) binding.2. The protein must exhibit XTP diphosphatase activity, ITP diphosphatase activity, metal ion binding. The designed protein sequence is ",
    "response#1": "SCRRP",
    "response#2": "GHHHH",
    "response#3": "MPCSPT"
  },
  {
    "reference": "MTENRLAIIVLAAGQGTRMRSTLPKVLHPIAGRPLVGHVLTSASRLGAEHIEVVVRHERDRVAGAITQQFPDAVIVDQDDIPGTGRAVEVALDAIPDFDGDVLILSGDTPLADAATLSAFLEEHRAAQAAATLMTAVVDDPTGYGRVIRDGGGDVDRIVEQKDATAEEAAVSEINAGMYVFRAAALREYLPAVGVDNAQGEKYLTDVPALLRRAGQRVAASVVSDVSVTFGVNDRVQLAQVGRLLNARIVRRWQLEGVTVVDPATTWIDDDAKLAPDVTLLPNTTIAGATVIDTGATIGPDTSLVDCEVGAGAVVRRTDATLAVIGAGANVGPFAYLRPGTTLGANGKIGTFVETKNSQIGEGSKVPHLSYIGDTTIGERVNLGAGAITANYDDLVKHRTEIGDEVHTGSHNVFVAPVRIGDGAKTGAGAVVRKDVPAGALALSVAPQRNVEGWVEKNRAGTGAAEAAARAGEAASRGTAE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.2. A protein that is capable of catalyzing reactions within the Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway with high efficiency and specificity.3. The designed protein should be involved in UDP-N-acetylglucosamine biosynthetic process, cell wall organization, peptidoglycan biosynthetic process, lipid A biosynthetic process by localizing to cytoplasm and exhibiting glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity, magnesium ion binding.4. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain..5. The presence of MobA-like NTP transferase domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "IILAAGQGTRMNSDLPKVLHEVAGAPLLVHAMTAGATIAADRMIVVTGHGADAVAAAARAHDETVEIAHQAEQRGTGHAVAAALPLLEGFEGRVIVLFGDTPFIRTGTLEAMLAASSDVVVLGFEAADPGRYGRLVTDGDGRVERIVEAKDATPEEAAVRECNGGIYVFDTAALAEVLPRLSTDNDQGEEYLTDVLGLLREQGRPVSAHLAPDADAALGVNTRVHLAEAGRELRARIVERWQRAGVTLIDPDSTYIEDTVKIGRDSLIEPGAVLKGNTVLGEDVRVGAYSVIEDSLVHDGVTIKSHTVLEGAEVHSGADAGPFARLRPGAVLEKDVHVGNFVEVKNTRMLEGSKAGHLTYLGDATVGREVNVGAGTITANYNGIGKFKTVIGDGAFIGSNSVLVAPVEVGAGAMVAAGSVVTQDIPEGSLGVARGRQRNVEGWVERRRPGSAAAQAASRAQEDAS",
    "response#2": "VIVLAAGEGTRMKSRTSKVLHELCGRSMLGHVLAAARELDPGRLIVVVGHRREQVAAHLAEHVPAAEPVVQERQGGTGHAVRTVLDQVGALTGTVVVTNGDHPLLRGETLAELVRTHETEGNAVTVLTTVVADDPTGYGRIVRDEGGAVTAIVEHKDASDAQRAIREVNSGVYAFDAGVLRDALAKLTTDNAQGEKYLTDVLGIAREAGRPVGAHLAADVWQTEGVNDRVQLATLGAELNRRTVTAHQRADAVVGPDNKIGPFARLRPGADLAEDVHIGNFVEIKERAWQARRRAQAMADGATLIAPETVWFSHDTSLGRDVTIEPNVVFGPGVTVADGATIHAFSHLEGASVGASCSVGPYARLRPGAVMQAKSRVGNFVEMKKAVLGEGAKANHLTYLGDAEVGAGANIGAGTITCNYDGFFKYQTKIGAGAFIGSNSALVAPVTIGDGAIVGAGAVVTRDVSADALALVRPAGVTIVDPLSTWIDVEVTAEPDVVIHPGTQLHGRTHLAEGAEVGPHSTLTDTLVGADAVVANTVCVEAEIGPEATVGPYAYLRPGTRLARKAKAGAYVEMKNAVVGEGSKVPHLTYVGDAEIGEGSNIGASCVFVNYDGVQKHRSVVGDHVKVGSDSMIVAPVTIGDGAYTAAGSVIVKDVPPGAMAVARGRQRNIEGWVARKRPGTPAAEAAERALAATGADADSTDEG",
    "response#3": "VIVLAAGAGTRMVSDTPKVLHELGGRSMLAHCLSAAAGVGPRHLVVVLGHEHGRITGPVAELAQALDFPIDIALQDQQLGTGHAVLCGLAALPDDFTGTVVVTSGDIPLLDTATLQALIDTHRTAPAAVTVLTATLDDPSGYGRILREADGSVQRIVEQKDASAAELAVREINTGVLAVPTRHLRDWLGRLQNQNAQGEYYLTDIIAMAVADGVSVVSAQPGSVWETEGVNNRLQLAALERAHQLRQAEAMMLAGVRLADPARIDVRGSLVCGRDVFIDVNCVFEGQVVLDEAVEIGPNCVLTNARIGAGTRLAAFTHIEDAVVGPDNKIGPFARLRPGADLAEDVHIGNFVEIKKTSIAAHSKVNHLAYIGDATIGRRVNVGAGTITCNYDGANKFQTIIEDDAFIGSDTQLVAPVTVGRGATLGAGTTLSEDAPPDTLTLTRPKGPVRRIAGWARRRRPD"
  },
  {
    "reference": "MQYKNLGRSGLRVSQLSYGAWVTFGNQLDVKEAKALLQACRDAGVNFFDNAEVYANGRAEEIMGQAMRDLGWRRSDVVVSTKLFWGGQGPNDKGLSRKHIVEGLRGSLKRLDMDYVDVVYCHRPDATTPVEETVRAMNWVIDHGMAFYWGTSEWSAQQITEAWSVANRLDLVGPIVEQPEYNLFSRHKVESEFLPLYSTYGLGLTTWSPLASGVLTGKYAKGNIPADSRFALENYKNLANRSLVDDTLRKVNGLKPIASELGVSLAQLAIAWCASNPNVSSVITGATKENQIVENMKALDVIPLLTPEVVDKIEAVVQSKPKRTESYR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Probable accessory potassium channel protein which modulates the activity of the pore-forming alpha subunit.2. A protein with voltage-gated ion channel activity functions is required to enable potassium ion transport, regulation of ion transmembrane transport. The designed protein sequence is ",
    "response#1": "MIAVVSVSVIVISIVIFCLETLPQFREDTSANLPLSNHHTTNGTTLHKKPNLFTDPFFMVETLCIVWFSFEFLVRFLSCPSKPAFFKNAMNSIDILAIAPYFITLGLELAEQQEAGSEQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLHASISELGLLIFFLLIGVILFSSAVYFAEADDPESGFSSIPAAFWWAVVSMTTVGYGDMCPVTIGGKIVGSMCAIAGVLTIALPVPVIVSNFNYFYHRS",
    "response#2": "MIAVVSVSVIVISIVIFCLETLPQFREDTSANLPLSNHHTTNGTTLHKKPNLFTDPFFMVETLCIVWFSFEFLVRFLSCPSKPAFFKNAMNSIDILAIAPYFITLGLELAEQQEAGSEQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLHASISELGLLIFFLLIGVILFSSAVYFAEADDPESGFSSIPAAFWWAVVSMTTVGYGDMCPVTIGGKIVGSMCAIAGVLTIALPVPVIVSNFNYFYHRI",
    "response#3": "RVVAFASLFFILVSITTFCLETHEAFNIDRNVTEIHRVGNITSVRFRREVETEPILTYIEGVCVMWFTLEFLVRIVCCPDTLDFVKNLLNIIDFVAILPFYLEVGLSGLSSKAARDVLGFLRVVRFVRILRIFKLTRHFVGLRVLGHTLRASTNEFLLLIIFLALGVLIFATMIYYAERIGARPSDPRGNDHTDFKNIPIGFWWAVVTMTTLGYGDMYPKTWSGMLVGALCALAGVLTIAMPVPVIVNNFGMYYS"
  },
  {
    "reference": "MASTGEPDRKRRHFSSISPSEAAAAVKKQPFFWPSSEDKLDTAVLQFQNLKLSQKLEAQQVECSILEDKLSQIKEKQLPYNSSLKTVHKSWEKLTASVESCSVRVSDSSSGAHRFVNKEDGSSPAVKNDFINRLLETGATESSSSNICSNQMEENGVNTSSQMTQTLYNLVAATEDLRCLKDELYPTVLRTNLGKDLCGQLALSELESEIKSFRGDLDDVLVKFKSLSRELQSHRDADAKVRVDLKRIRGELEDEVVELQQCNGDLSALRAERDATAGAFFPVLSLGNKLATSDRERDKQRDLQDMETVLKELTVLASGRLQQLKNLHEERTKMLGKMSNLQNKSKSVRCISSSQACLSLKDQLEKSKEAVFQYMALLEKLQVEKDSIVWKEREINIKNELGDVSRKTSAVTDSRMASLDSEIQKQLDEKMRIKTRLGNISRERGRKEIFADMKALISSFPEEMSSMRSQLNNYKETAGGIHSLRADVQSLSGVLCRKTKEYEALQLRSADYASQLGDLNATVCDLKNSHEELKLFLDMYKRESTDARDIAEAKEQEYRAWAHVQSLKSSLDEQNLELRVKAANEAEAVSQQMLAAAEAEIADLRQKMDDCKRDVAKHSDILKSKHEEHGTYLSEIQTIGSAYEDIVPQNQQLLLQVTERDDYNIKLFLEGITSRQMQDTLLIDKYIMDKDIQQGSAYASFLSKKSSRIEDQLRFCTDQFQKLAEDKYQKSVSLENLQKKRADIGNGLEQARSRLEESHSKVEQSRLD",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. We require a protein that can specifically bind to the metabolites produced in the protein ubiquitination pathway.2. The protein must exhibit the following characteristics: Involved in the control of seed dormancy and germination.; It thereby plays a central role in histone code and gene regulation; Required for the regulation of flowering time and defense against necrotrophic fungal pathogens.3. The designed protein should have metal ion binding, identical protein binding, ubiquitin-protein transferase activity within mitochondrion, HULC complex, nucleus to regulate regulation of G2/M transition of mitotic cell cycle.4. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "NFPTATQEYVCPSHGSHVICTCCFQPMPDRRAEREHNSHVAPQQCTICLEPFCHMYWGCNRMGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLASRGLTWKDMLNESLAAVQRGVFMLPDYRINGTTVLCYFCGLRNFRILTYQYRQNC",
    "response#2": "NFPTATQEYVCPSHGSHVICTCCFQPMPDRRAEREHNSHVAPQQCTICLEPFCHMYWGCNRMGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLASRGLTWKDMLNESLAAVQRGVFMLPDYRINGTTVLCYFCGLRNFRILTYQYRQNC",
    "response#3": "NFPTATQEYVCPSHGSHVICTCCFQPMPDRRAEREHNSHVAPQQCTICLEPFCHMYWGCNRMGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLASRGLTWKDMLNESLAAVQRGVFMLPDYRINGTTVLCYFCGLRNFRILTYQYRQNC"
  },
  {
    "reference": "MPHWITHPSELTDRLQAARPARIGLDTEFIRERTYWPQLALVQMAIGEEILLIDPLIPGMNAALKEWLTATDIVKVMHSASEDLVTFKCACGVLPRPLFDTQIAAALAGVGGGMGYQKLVQEVTGTLLTKGETRSDWMRRPLSPSQLEYAADDVRYLFAIHDELTRRLTEQNRLGWLAEDAERLLATVEHDDGERWPHVSLRTAQFLEPAAQRRLLRLLRWRDLQARQSDRPRSWILDNEVASQLARFPPADLDALLQQFDKFPKAPRKLANAVWDALNTPLPDEEHAPLAQAATDGNKAVLKRLQDTVAQRSHELGLPDGLLASRRHLETLIEQRSWPAALGQWRRAVLEAQVMPLLEASEA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. We are looking for a protein with broad substrate specificity for Exonucleolytic cleavage that removes extra residues from the   3'-terminus of tRNA to produce 5'-mononucleotides..2. The ideal protein would be one that plays a critical role in tRNA 3'-end processing through nucleotide binding, nucleic acid binding, 3'-5' exonuclease activity within cytoplasm. The designed protein sequence is ",
    "response#1": "HWITHPSELTDRLQAARPARIGLDTEFIRERTYWPQLALVQMAIGEEILLIDPLIPGMNAALKEWLTATDIVKVMHSASEDLVTFKCACGVLPRPLFDTQIAAALAGVGGGMGYQKLVQEVTGTLLTKGETRSDWMRRPLSPSQLEYAADDVRYLFAIHDELTRRR",
    "response#2": "PHWITHPSELTDRLQAARPARIGLDTEFIRERTYWPQLALVQMAIGEEILLIDPLIPGMNAALKEWLTATDIVKVMHSASEDLVTFKCACGVLPRPLFDTQIAAALAGVGGGMGYQKLVQEVTGTLLTKGETRSDWMRRPLSPSQLEYAADDVRYLFAIHDELTRRLV",
    "response#3": "HWITHPSELTDRLQAARPARIGLDTEFIRERTYWPQLALVQMAIGEEILLIDPLIPGMNAALKEWLTATDIVKVMHSASEDLVTFKCACGVLPRPLFDTQIAAALAGVGGGMGYQKLVQEVTGTLLTKGETRSDWMRRPLSPSQLEYAADDVRYLFAIHDELTRRFS"
  },
  {
    "reference": "MTTETRQKRIGIFDSGIGGLTVLRELYRQLPKESILYFADTARLPYGTRTTEEILQFVDEIITWLVKSGVKMVLMACNTSSALALEKVKSKFDVPILGLIVPGANTAVKQGKRIGVIATPATAASNAYRYAILEANASVQVWQVGCPYFVPLIEQNQLHDPYTYQIAEEYLMPLIQQQIDTLVYGCTHYPYLEPILRSLLPKSVIFVDPAVSLVKVVARELKIMNLENDKTPKPTKFVVSSSPQKFADLSLQLLGYKPDVQVISLPAMLK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should aim to mimic or enhance the function of natural proteins involved in peptidoglycan biosynthetic process, cell wall organization, regulation of cell shape, potentially with improved properties such as stability, specificity or selectivity.2. The protein design should take into account the physiological conditions of the Cell wall biogenesis, peptidoglycan biosynthesis pathway.3. The protein must exhibit the following characteristics: Provides the (R)-glutamate required for cell wall biosynthesis..4. The designed protein must have a Proton donor/acceptor active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "ITGTNGKTTVSYLIEAMLLKAGYLPAVIGTVNYRFKGRSMPAGNTTPGPLEIQKMLADIAREHGTHLVMEVSSHALHQERTEGVDFSSAIFTNLTQDHLDYHGTMEGYFAAKLKLFQGLTKSAFAVINNDQEQADRIKKATRAKVVTYGIDKAADVRAQGMRFSAHGTEFTAEAGSHTVYVRSSLIGRHNVYNMLAAIL",
    "response#2": "VTGTSGKTTTAYLVEAGLRAAGLATGLIGTVETRIGDERLASAFTTPEAPDLQALLAVMVEQGVTHVPMEVSSHALALGRANGTRFAVGAFTNLSQDHLDFHRDMEEYFAAKSLLFDGRSTHEVVVVDNAWGQSLVTPQTVTVSTEPGTDASWRATDISITSTGEQSFTLHAPDGRVAEATLPLPGEFNVANATLAAAG",
    "response#3": "VAGMHGKTTTSAMTAHVLREGGLHPSHYVGAEIPILSTNAHWDARGEWFVAEGDESDGTLELFHPEHALILNIEEEHLDFYADLAAIEKIFARLIEQTAGTVFYNIDDASTVPLCATRKNTISFGFADTADYRGTEVDLQAFSSNFCVYSRGKKLGEVVLNVPGRHNVHNAIGVVALAEELG"
  },
  {
    "reference": "MLIAVIGVNHRTAPLEVREKLAFTEWGMKDSLKRLMSYPGIDGCAIVSTCNRTEIYIAPMELDSGMSSVWSFLSEKSGLDISEIKNFTFCHTLYDAIRHLFRVVSGLDSMILGETQILGQVKKAYELALEAKTTNVVLNTLFQQAITTGKRVRTETGIDQNPVSIPYAAVELAKQGFGTLSGRSVLVVGAGEMSELTVVNLVANGVSSVIVSNRSYDRAVQLAEKFDGTAVKFDQLFQYMNRADIVISSTAAQHYVIKTSDMQKVMEQRVDQPIMMIDIAVPRDIDPEVRRIPNVKLYDVDHLQNVVDANLEERRRAAVQAEGIIEEELNEFMRWLSTRFVVPTITALKKMGEEIKQRELVRAYNRLGDLTEREKKIIGSLANSIVNQLLHNPVIKLKQYALTPEGHLYTELTQNIFNLQVEGQKPQTEIIPPEKKKKVAGGSTLNSGWDKGIAAMVADSRSRE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The desired protein should be able to perform (S)-4-amino-5-oxopentanoate + NADP(+) + tRNA(Glu) = H(+) + L-   glutamyl-tRNA(Glu) + NADPH2. We require a protein that can specifically bind to the metabolites produced in the 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2, protoporphyrin-IX biosynthesis pathway. The designed protein sequence is ",
    "response#1": "GLTGRTAVVVGAGSMGGLSVAHLTRAGIGRIVVVNRTRERAEHLADTARSNGVAAEALELAELPDAMAQADVLVTCTGAVGAVVTLADTHRALAQPGRDSERPLVICDLGLPRDVEPAVSGLPGVTVLDMESLR",
    "response#2": "KAYEIVGLATMEFYQWIQSLEVEPVIKDLRELARISAQKELQKALKKRYVPKEYESNIEKILHNAFNTFLHHPTIALKKNAQKEESDVLVGAIKNLFNA",
    "response#3": "VAEKKVGPLAGRSAVVVGAGSMGALAATQLARAGIERIHVVNRSLPRGKRLAENIRGYGIAAEAYPFDHLPPLLTDADIVVSCTGAVRPVVSLADVHRGLAHVREPKDLVICDLGMPRDVDAAVAGLPGVFVIDMDR"
  },
  {
    "reference": "MASQYAAELCMLLVEDNFGELFGRIFSTLHRYERLTLPRLLGLTRLSENRLRAALVAMIQHHLVHFYTAVEEGGVTYYSANMQAAYYLIRSGKILEFVEDRLGKYAATVMSTIMYLGHAQVSHLDTLPELRSDASKTNGVNEGEHDRPEGENETNGTNGEHVSDQPALLHPTLKALAGHGYIVRVREAHFQSIADNILNIERQIRSGDSVQSMKGKKLEEFVVEKTADMLKERLDGDLTRGLIVNGVPRGVKRSQTNGVSDDNETEKARFDYDDGEDEDEENEWLDDEVPMESSLTVRVNYEKLDVALRNRRFLELAERGAPPESAQIYDYLLRRIEYQTSKCRDASEIPREGEEGEQYSVPIPLSALARDIDPYLDLAGSLGPTNTPANNRLGKRTFDDTVDDNDDDHGATGNRTYEIDQHLSLLAQPPLNLTSKRLNGGIINWTVEFRHLARKLRHLELERIVEARYGDVALRVLRVLQAKGKLDEKRLQEISLLPFKDLRQVLASMQTGGFVDLQEVPRDAQRQPSRTIYLWYYDPDRARDSMLQDTYKAMSRCLQRLKFERGRLKDFLEKTERSDVKGNEELYLSEGELDRLREWRAKEALLIGEVARLDDMIAVMRDY",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs .; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The designed protein sequence is ",
    "response#1": "TLIQLLEEGFIQPVGQNMFRSPTDSYNAVEKALLQDSYGGATRGTKQKDELRMRIRGQLQELRAQVPNWKPVGYNRSSTNGHTNDIASKRRRLSHSGGATNGYDFGDDESSKLDGNLVLRINHEKCTVFMRNRRLVELANSRIGVTTSYIYAELLRLMAEQIPRCRPDPRIDDAVDDADGPSIIITTQELTDALSKTINVSTGIGKATSQKIDTSRLDKLQNGRKRKAQDEAEVEGVASSDEESEDDHKPFTNGNGHAMDVDEDDPFSDQPGANTSKRAVTFKDRDRTPPPTESRQARMMHVMSHLQLLAADDCQLLRKCGARQMGEWTVDFERVIDRR",
    "response#2": "LDSIIYENFGQIGHRLVRVMRKMGKLEEKHIAKLALIKQQDSRTTLVNMQMHGMVDIQEVPRDTGRMIVRTIHLWG",
    "response#3": "LDSIIYENFGQIGHRLVRVMRKMGKLEEKHIAKLALIKQQDSRTTLVNMQMHGMVDIQEVPRDTGRMIVRTIHLWK"
  },
  {
    "reference": "MSYSLLFLLALQFCLGSASRTTLTSAHSRELTTPPTSPQATAAWLPPGGTSWAEGGTVSQPLSNFTGSVDSHGTCQCSVSLPDTAFPADRVERLEYTAHILSQKFEREFSKVKEYVQLISVYEKRLLNLTVRVEVMEKDSISYTELDFELIKLEVKEMQKLVLQLKKNFVGSTHIIDMLEVEIRNMTLLVEKLESLDQNNVLSIRRQILALKTKLKECEASKSDLVPATPPPPAPGSCSHGGVVNISAPSVIQLNWLGFSYKYGAWGRDYSPQHPERTLYWVAPLNTDARALEYYRLYDSLDNLLIYSHFRDYRIRYGQGGGTVAFNNNLYVNWYNGGNIAKINLTTNVVDVNRPLPLAAYNNRFSYANVNWQDIDLAVDEQALWAIYATEASTGNIVISKLNDTTLEVISTWVTKQYKPSVSNAFMVCGVLYATRTLNTKTEEIFYYYDTNTEREGNLGITMRKMQERIQSINYHPFDQKLYVYNDGYLLNYDLVFLQTPRQPV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be expressed specifically in the protein-containing complex2. The protein must exhibit the following characteristics: In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. The designed protein sequence is ",
    "response#1": "AQGLETLFSKAQELGGASQEDPDLWPLLCRLADSKSYTAYSEALAELRSQAPTAFIRYFEHNWAPRHDMWVRFRAYEATRDLDACALVRSHRRRLLRRLSPSHSVAQCLRDLVAMQG",
    "response#2": "AQGLETLFSKAQELGGASQEDPDLWPLLCRLADSKSYTAYSEALAELRSQAPTAFIRYFEHNWAPRHDMWVRFRAYEATRDLDACALVRSHRRRLLRRLSPSHSVAQCLRDLVAMQL",
    "response#3": "VRITNQISKQFVAPADVRRLLTHCKGPDHGVLDALQVLEGLFRTDPEAKVKLVFVEDQAMVETVFLLTSRTRALLRRFPRILLVDRLPGLQGTLDLMAVLCVDSAGRARQAACCVARPGTPSLLRFMLVSLLQSAPDVKGRVRCLTAGPEVAGQLRAVRQLLPCARVQIL"
  },
  {
    "reference": "MVRLGSYCEHNGSISQAWLDSGLSPCFYFTLVPSVLLSFSFLLGALQSALYARHSTTMEPKYIPRSRLYRLQIVLSVVLILQSVIGLIWQAAGTDVVYGYMIVHGCLSVVAWGFSLWLLHLERTRALVREKSRGHGVVLLLFWALAFAAENLAFISWQSPNWWWLSRDTVPQKVQFGLWITRYVCTLFLFVLGIRAPGRPRKPYIVLINEDERDVETSQPLLRDPNQSTWQGFKKKLLLVMQYIWPRRNIPLQLLVALCMGLMGLERAINVFVPIYAKKIVDGLTEDSTWNILAVTVCIYVLLKFLQGGGAGTTGFLSNLRTFMWIRVQQFTNREVQIRLFAHLHSLSLRWHLGRKTGEVLRSVDRGTSSINSLLSYIVFSILPTIADIVIGIVYFTSSFNAWFGLIIFVCMTLYLTLTIIITEWRTKYRREMNTRDNEAKSRAVDSLLNFETVKYYNAEGYEVGRFNDSIMKYQVSEWKVNASLAMLNQTQNLIIGLGLLAGSLLCAYFVTENKFKVGDYVLFGTYIIQLYTPLNWFGTYYRMIQSSFIDMENMFELFNEDQEVKDAVNAPALMFRSGKIEFENVHFSYLDGKEILRDISFTVMPGQSIALVGPSGSGKSTIIRLLFRFYDVKGGTIKVDGQDISTVRQESLRSHIGVVPQDTVLFNDTIRNNIRYGRVSATDDEVEEAAAAADIHERILSFRDGYDTQTGERGLKLSGGEKQRVAIARTILKAPQIILLDEATSALDTETERNIQASLAKVCANRT",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be involved in cellular detoxification of cadmium ion, heme transmembrane transport, porphyrin-containing compound metabolic process, heme metabolic process by localizing to late endosome membrane, Golgi apparatus, early endosome membrane and exhibiting ATP hydrolysis activity, ABC-type transporter activity.2. The protein must exhibit the following characteristics: May also play a key role in the early steps of melanogenesis producing PMEL amyloid fibrils; In addition may play a role in the transition metal homeostasis .; ATP-dependent transporter that catalyzes the transport of a broad-spectrum of porphyrins from the cytoplasm to the extracellular space through the plasma membrane or into the vesicle lumen; In vitro, it confers to cells a resistance to toxic metal such as arsenic and cadmium and against chemotherapeutics agent such as 5-fluorouracil, SN-38 and vincristin ; May also function as an ATP-dependent importer of porphyrins from the cytoplasm into the mitochondria, in turn may participate in the de novo heme biosynthesis regulation and in the coordination of heme and iron homeostasis during phenylhydrazine stress. The designed protein sequence is ",
    "response#1": "LERALNVLVPIFYRNIVNLLTEKAPWNSLAWTVTSYVFLKFLQGGGTGSTGFVSNLRTFLWIRVQQFTSRRVELLIFSHLHELSLRWHLGRRTGEVLRIADRGTSSVTGLLSYLVFNVIPTLADIIIGIIYFSMFFNAWFGLIVFLCMSLYLTLTIVVTEWRTKFRRAMNTQENATRARAVDSLLNFETVKYYNAESYEVERYREAIIKYQGLEWKSSASLVLLNQTQNLVIGLGLLAGSLLCAYFVTEQKLQVGDYVLFGTYIIQLYMPLNWFGTYYRMIQNSFVDMENMFDLLREDQEIVDAPGAGPLSVGRGDIEFENVHFSYADGRETLQDVSFTVMPGQTLALVGPSGAGKSTILRLLFRFYDISSGCIRIDGQDISQVTQASLRSHIGVVPQDTVLFNDTIADNIRYGRVTAGNDEVEAAAQAAGIHDAIMAFPEGYRTQVGERGLKLSGGEKQRVAIARTILKAPGIILLDEATSALDTSNERAIQASLAKVCANRTTIVVAHRLSTVVNADQILVIKDGCIVERGRHEALLSRGGVYADMWQLQKYS",
    "response#2": "LLCMGLMGLDRALNVLVPIFYRDIVNLLTSKAPWSSLAWTVTTYVFLKFLQGGGTGSTGFVSNLRTFLWIRVQQFTSRGVELRLFSHLHELSLRWHLGRRTGEVLRIVDRGTSSVTGLLSYLVFNIIPTLADIIIGIIYFSMFFNAWFGLIVFLCMSLYLILTIMVTEWRAKFRRDMNTQENATRARAVDSLLNFETVKYYNAEGYELERYREAILKFQGLEWKSTASLVLLNQTQNMVIGFGLLAGSLLCAYFVSERRLQVGDFVLFGTYITQLYMPLNWFGTYYRMIQLLCMGLMGLDRALNVLVPIFYRDIVNLLTSKAPWSSLAWTVTTYVFLKFLQGGGTGSTGFVSNLRTFLWIRVQQFTSRGVELRLFSHLHELSLRWHLGRRTGEVLRIVDRGTSSVTGLLSYLVFNIIPTLADIIIGIIYFSMFFNAWFGLIVFLCMSLYLILTIMVTEWRAKFRRDMNTQENATRARAVDSLLNFETVKYYNAEGYELERYREAILKFQGLEWKSTASLVLLNQTQNMVIGFGLLAGSLLCAYFVSERRLQVGDFVLFGTYITQLYMPLNWFGTYYRMIQSNFIDMENMFDLLDEEQEVKDEVNAGNLIYKQGKVEFENVHFSYADGRETLQDVSFTVMPGQTVALVGPSGAGKSTILRLLFRFYDISSGCIRIDGQDISQVTQISLRSHIGVVPQDTVLFNDTIANNIRYGRVTAGDSEIQAAAQAAGIHDAILSFPEGYETQVGERGLKLSGGEKQRVAIARTILKAPDIILLDEATSALDTSNERAIQ",
    "response#3": "FCMVLMGLERAINVFVPIYYKNIVNELTGATPWPALAWTVCTYSALKFLQGGGAGSTGFVSNLRTFLWIRVQQFTNRQVQVQLFGHLHGLSLRWHLGRKTGEVLRSIDRGTSSVNSLLSYIVFSIFPTVADILIGIVYFTSAFNAWFGLIIFVCMSLYLTLTILITEWRTRYRRDMNTRDNEAKSRAVDSLLNFETVKYYNAESYEVTRFNDAIIKYQVSEWKVSASLALLNQTQNLIIGLGLLAGSLLCAYFVTEKKFQVGDYVLFGTYIIQLYTPLNWFGTYYRAIQKNFVDMENMFELLREDQEIVDAPGAGELHVRRGGIEFENVHFSYADGRETLQDVSFTVMPGQTLALVGPSGAGKSTILRLLFRFYDISSGCIRIDGQDISQVTQASLRSHIGVVPQDTVLFNDTIADNIRYGRVTAGNDEVEAAAQAAGIHDAIMAFPEGYRTQVGERGLKLSGGEKQRVAIARTILKAPGIILLDEATSALDTSNERAIQASLAKVCANRTTIVVAHRLSTVVNADQILVIKDGCIVERGRHEALLSRGGVYADMWQIQFQQEDQVEEVA"
  },
  {
    "reference": "MKVTVCFGRTGIVVPCKDGQLRVRELTQQALQRYLKTRDQDPGYWVKIHHLEYTDGGILDPDDVLADVVEDKDKLIAVFDEQEPLQKTESPGGNPADRQSPDAFETEVAAQLAAFKPVGGEIVVTPSALKLGTPLLVRRSSDPAPGPHADAQPSTASLSGQSLKPVVLDSTQNVENKEAMNGEQAGLLSLHRPKDELSDMTRAVEISGEGDPLGIHVVPFFSSLSGRILGLFIRGIEENSRCKQEGLFQENECIVKINNVELLDKTFAQAQDVFRQAMKSPSVILHVLLPQNREQYEKSVIGPLNIFGNNDGASRTKAAPPARGKPGLKAVHLTRASSPEGEEPASPQQSKSPRVPRLGRKPSSPSLSPLMGFGSKKNAKKIKIDLKKGPEGLGFTVVTRDSSIHGPGPIFVKNILPKGAAVKDGRLQSGDRILEVNGRDVTGRTQEELVAMLRSTKQGETVSLVIARQEGSFLPRELKGEPDCYALSLESSEQLTLEIPLNDSGSAGLGVSLKGNKSRETGTDLGIFIKSIIHGGAAFKDGRLRMNDQLIAVNGETLLGKSNHEAMETLRRSMSMEGNIRGMIQLVILRRPERPLEELSECGALSRPGFENCQEALSTSRRNDSSILYPFGTYSPQDKRKDLLLPSDGWAENEVPPSPPPHPALEWGLEDFSHSSGVDSTGYFPDQHVNFRTVTPVRQPELINLKASKSMDLVPDEGKVQSLADRRSDSPGKDFGPTLGLKKSSSLESLQTAVAEVRKNDLPFHRPR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: May play a role in the formation of epithelial tight junctions .; Putative adapter protein involved in asymmetrical cell division and cell polarization processes.2. The designed protein should be involved in microtubule cytoskeleton organization, cell division, cell cycle, establishment of centrosome localization by localizing to bicellular tight junction, cell cortex, apical plasma membrane, protein-containing complex, apical junction complex and exhibiting phosphatidylinositol binding.3. Target a Basic and acidic residues, Pro residues, Polar residues, Basic residues compositional bias in the protein's composition for improved properties.4. The protein contains novel PDZ domains that confer a unique function or activity.5. The protein design should prioritize the formation of strand-strand-strand-turn-strand-turn-strand-strand-helix-strand-strand-helix-strand elements. The designed protein sequence is ",
    "response#1": "MEVKSKFGAEFRRFSLERSKPGKFEEFYGLLQHVHKIPNVDVLVGYADIHGDLLPINNDDNYHKAVSTANPLLRIFIQKR",
    "response#2": "MKVTVCFGRTRVVVPCGDGHMKVFSLIQQAVTRYRKAIAKDPNYWIQVHRLEHGDGGILDLDDILCDVADDKDRLVAVFDEQYLRVPSHLQAETLVGKVNLEECLKSASLIRSSDPAFRILEDGSIYTTHDLILSSERKSFSIFLSDGQRREQQEIKVPLPQIDKEVTICQNNEDFAVLKPVDPDGPENGPPFQFFLDNSASKNWNIEEKDGKTAILRQRQNLDYNYYSVPIQIKDRHGLVATHMLTVRVCDCSTPSPSFTETSYVTEVEEPTVSLIKGPDSLIDGGNETVAAVCVAATGKPVAQIDWEGDLGEMESSTTSFPNETATIVSQYKLFPTRFARGRRITCVVKHPALEKDIRYSFILDEPHVTAVWGKNVSLKCLIEVNETITQISWEKIHGKSTQTVAVHHPQYGFSVQGDYQGRVLFKNYSLNDATITLHNIGFSDSGKYICKAVTFPLGNAQSSTTVTVNDSGSAGLGVSVKGNRSKENHADLGIFVKSIINGGAASKDGRLRVNDQLIAVNGESLLGKTNQDAMETLRREPPLLLLLIPLLLFSRLCGALAGSIIVEPHVTAVWGKNVSLKCLIEVNETITQISWEKIHGKSTQTVAVHHPQYGFSVQGDYQGRVLFKNYSLNDATITLHNIGFSDSGKYICKAVTFPLGNAQSSTTVPTVSLIKGPDSLIDGGNETVAAVCVAATGKPVAQIDWEGDLGEMESSTTSFPNETATIVSQYKLFPTRFARGRRITCVVKHPALEKDILVRGP",
    "response#3": "MKVTVCFGRTRVVVPCGDGHMKVFSLIQQAVTRYRKAIAKDPNYWIQVHRLEHGDGGILDLDDILCDVADDKDRLVAVFDEQPTVSLIKGPDSLIDGGNETVAAVCVAATGKPVAQIDWEGDLGEMESSTTSFPNETATIVSQYKLFPTRFARGRRITCVVKHPALEKDIRYSFILDD"
  },
  {
    "reference": "MARPKNVKHIFVTGGVISSLGKGILSASLGLLLKSRGLRVAIQKYDPYINVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFLDEPTSQACNLTMGRVYKSVIDKERRGEYLGGTVQVVPHVIDEIKEKMGDLAKNGSIDVLITEIGGTIGDIESLPFLEAMRQLKLELGEHNLLNIHLTFVPYIKAASELKTKPTQHSVKMLLETGIQPDILVCRSEKPLSREIKNKVGHFCNVHDLDVIGLNDCDTIYEVPLMLLKEQLDLRVMKKLGLKKFREPNLEYWKNFCEKVKHPKDGEITIGICGKYTEYPDAYKSIIESFIHAGASNDVRVLVKMLRAEDAEDPKFDISSAFKGISGLLVAPGFGDRGIEGKVRFVQYARENNIPFFGICLGMQCATIEFARNICDLPDANSTEFNKRTRFPVIDLMEHQKKVKEKGGTMRLGSYPCILKEGSKAHELYGKFLINERHRHRYEFNNQFRKLFEEKGMIFSGTSPNGDLVEIVELKNHRWFVAVQFHPELKSRVQKVHPLFDGFVHAAKEFAQGKRQLSLEVEMPRLSSTEMENAG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for CTP binding.2. We require a protein that can specifically bind to the metabolites produced in the CTP biosynthesis via de novo pathway pathway. The designed protein sequence is ",
    "response#1": "LQDAYLSVVEALKHAGYAYDADIDINWVNAEHVTEENVEELLGDSDGILVPGGFGDRGVEGKITAIKYAREKKVPFLGICLGMQLATVEYARNVLGLKGAHSAELDPSTKDPIIDLLPEQKDVEDLGGTLRLGLYPCKLVEGTKVHEAYGEKVVYERHRHRYEFNNHYRQDMERAGFVFSGTSPDGRLIETIEITDHPWFVASQFHPEFLSRPNRPQPLFREFVGHA",
    "response#2": "VTVALVGKYIDLPDAYLSVAEALRAGGFAHEAKVHLRWIASDECETPQGAAKHLHDVDAVLVPGGFGIRGLEGKLGALTYARTHGIPTLGLCLGLQCMVIEYARNVAGIEGAGSTEFETDVEHPVISTIEEQKKYVEGAGDLGGTMRLGLQPADLVPGSVAAKAYGTERVEERHRHRYEFNDAYRAELEEAGLVFSGVNPDLGLVEFVELPADVHPYYVSTQAHPELKSRPTRPHPLFAGLIEQAIERAGTKAGNKGFEVA",
    "response#3": "LRDAYISVVEALRHAGLHQELDIDIRWISSEQVEREGPERLLANVYGIVVPGGFGDRGIEGKIMAADYARTQEIPYLGLCLGMQCATIAFARQLIGNNEANSTEFDPHTPHPVIDLMPDQRDVTDKGGTMRLGIYPCVLKPGTRAAEAYGADEVMERHRHRFEFNNKYRALLEEAGFVISGHSPDNRLVEIIELRDHPWFVGSQFHPEFLSRPSRPHPLFREFVRD"
  },
  {
    "reference": "MSRKTSLPKICIALGFSEVDTLWKHARREVESGETFLEFRIDYLSNPLDGVHAIERFTRQFPECTLLATCRRHQNHGRFNGSVDEQIRLLDLAIDAGARAFDIEIESAEAAPAAVERLRSKAWAILSFHHWGGTPAVEPILRRMTRIPADGYKVVTTARKPSDNLRVLSLARTNPRTPLVMLAMGEPGLATRVLSPAFGAIYTYAAPNAAEGTAPGQISARVLRNIYHADKVTKAAKIYGVIADPVRHSISPAVHNRAFHSRRIDAVYLPFLVHAAQLKDFFILGAKLPLSGFSVTIPHKQKILRYLDGIDPLARRIGAVNTVTRRAGKWRGSNTDVEGVLTPLRKLITPAKSSVLVAGNGGAARGAAFALVEAGAKVSIVGRNPDRVRALAKACGATAVTREQVDSLYYDALIHATPVGMHPNVDDSFFPDRIPADIVFDMVYNPLETRLIRQAAASGKKVIPGHEMFIEQAVRQFELWTNETAPRAAMEKAALEALQARAEPIIEAK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. We need a protein that can perform NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH with high efficiency.2. For general function, the protein need meet that Catalyzes the cis- dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate.3. We require a protein that can specifically bind to the metabolites produced in the Metabolic intermediate biosynthesis, chorismate biosynthesis pathway.4. The designed protein should contain one or more SDH C-terminal domains that are essential for its function. The designed protein sequence is ",
    "response#1": "GMAEVVKTAAIWNEEEFISLESHPAEIFAAIQTPSKNYTGRSKATRSQAQELLLSVIVGSISVKAHIVTIDERETGLRNLVNFGHSVGHAIEAVLTPTILHGECVSIGMVLEGEISRQMGILSQVGVGRLTRCLKSYNLPVSVSDPRIANLPAAKQLTVERLLDIMRIDKKN",
    "response#2": "GLGEVIKHALLMSEDVLDGLETSVDSVVDREPAVVGQLVKMSVSYKSGVVSDDEREAGKRAVLNLGHTIGHALEVVAGYGVLSHGQAVALGLLAALAVSERLLGLDPSVRARTGALMERLGLCTSMVLPPTERLVAATARDKKGWVKKIN",
    "response#3": "GLGEVIKHALLMSEDVLDGLETSVDSVVDREPAVVGQLVKMSVSYKSGVVSDDEREAGKRAVLNLGHTIGHALEVVAGYGVLSHGQAVALGLLAALAVSERLLGLDPSVRARTGALMERLGLCTSMVLPPTERLVAATARDKKGMAEVVKTAAIWNEEEFISLESHPAEIFAAIQTPSKNYTGRSKATRSQAQELLLSVIVGSISVKAHIVTIDERETGLRNLVNFGHSVGHAIEAVLTPTILHGECVSIGMVLEGEISRQMGILSQVGVGRLTRCLKSYNLPVSVSDPRIANLPAAKQLTVERLLDIMRIDKKE"
  },
  {
    "reference": "MKFNEAPSADGVWHHCHLLPAADPAQAIRDGAIVVEQGRIAWLGAFAALPDVYRHLPRHDANGAWITPGLVDCHTHLVYGGQRADEFAMRLAGASYEEIARAGGGIVSTVRATREADEATLFAQASARLEPLLAEGVTAIEIKSGYGLSLEAERKQLRVARQLGETYGVAVHTTFLGAHALPPEYAGRADAYIELVCETMLPALAAEGLVDAVDAFCEGIGFSIAQTTRVFEAAARHGLPVKLHAEQLSNLGGAALAARHKAISADHLEHLDEAGVVAMAEAGTVAVLLPGAYYFLRDTQLPPLALLRKHGVPMAISTDHNPGTSPTTSLLLMMNMGCTIFRMTVPEVLAGVTTHAARALGAADRHGLLAVDRAADFVLWQVASPAELAYWFGRNPCAAVVRRGRVFRKGEGWQR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Fe(3+) is required for the proper folding and stability of the protein.2. The designed protein should have a high specificity for 4-imidazolone-5-propanoate, N-formimidoyl-L-glutamate, Fe(3+), Zn(2+) binding, with minimal off-target effects.3. The protein design should consider the endogenous proteins and enzymes present in the Amino-acid degradation pathway to avoid interference with native metabolic processes.4. A protein that contributes to histidine catabolic process to glutamate and formate by functioning in cytoplasm with zinc ion binding, imidazolonepropionase activity.5. The protein must exhibit the following characteristics: Catalyzes the hydrolytic cleavage of the carbon-nitrogen bond in imidazolone-5-propanoate to yield N-formimidoyl-L-glutamate; It is the third step in the universal histidine degradation pathway.. The designed protein sequence is ",
    "response#1": "MTPGLVDCHTHLVYGGQRADEFAMRLAGASYEEIARAGGGIVSSVRATRAADEDTLFSQAAARLAPLLAEGVTAIEIKSGYGLELEAERRQLRVARRLGEAFGVSVHTTFLGAHALPPEYAGRADDYIDLVCNTMLPALAEEGLVDAVDAFCESVGFSLAQTERVFEAAARHGLRVKLHAEQLSNLGGAALAARHHALSADHLEHLDEAGVEAMAAAGTVAVLLPGAYYFLRDTNLPPIALLRKHGVPIAISTDHNPGTSPVTSLLLMMNMACTLFRLTVPEALAGVTTHAARALGAPDRHGKLAVGRAADFVLWRVESPAELAYWFGRNPAAVVAAL",
    "response#2": "MTPGLVDCHTHLVYGGQRADEFAMRLAGASYEEIARAGGGIVSSVRATRAADEDTLFSQAAARLAPLLAEGVTAIEIKSGYGLELEAERRQLRVARRLGEAFGVSVHTTFLGAHALPPEYAGRADDYIDLVCNTMLPALAEEGLVDAVDAFCESVGFSLAQTERVFEAAARHGLRVKLHAEQLSNLGGAALAARHHALSADHLEHLDEAGVEAMAAAGTVAVLLPGAYYFLRDTNLPPIALLRKHGVPIAISTDHNPGTSPVTSLLLMMNMACTLFRLTVPEALAGVTTHAARALGAPDRHGKLAVGRAADFVLWRVESPAELAYWFGRNPAAVVL",
    "response#3": "MTPGLVDCHTHLVYGGQRADEFAMRLAGASYEEIARAGGGIVSSVRATRAADEDTLFSQAAARLAPLLAEGVTAIEIKSGYGLELEAERRQLRVARRLGEAFGVSVHTTFLGAHALPPEYAGRADDYIDLVCNTMLPALAEEGLVDAVDAFCESVGFSLAQTERVFEAAARHGLRVKLHAEQLSNLGGAALAARHHALSADHLEHLDEAGVEAMAAAGTVAVLLPGAYYFLRDTNLPPIALLRKHGVPIAISTDHNPGTSPVTSLLLMMNMACTLFRLTVPEALAGVTTHAARALGAPDRHGKLAVGRAADFVLWRVESPAELAYWFGRNPAAVVC"
  },
  {
    "reference": "MQIDLSGKSVLLGVTGSISAYKACDIARLFVKAGAQVNVVMTPSAERFVSALTFEALTRNPVLTESSESWSSDLNHIDIGKKCDVFVIAPATANTLNKLSKGIADNILTQTVLAFTKKILLSPAANTHMLANPFTTQSMQTLATNNYTIIQPQEKLLACRDVGNGALAEPSEIFFETAKALHEEEFWKDRPIVVTGGGSREKIDEVRYISNFSSGKMAKSLCLALYLKGAKVQYITSMDTEGLPSYIDTIHFEDADALLGDVQQAITTVKKENNKEAFLFMVAAVADFKPKFPQKGKLKKTALGETWNVELTQNPDILSSLDKTGIKTVAFKAEMDSEEGLNNAVKLLTQKSVDAVCYNLLKDAKSFGGEENEITFITKDTQIPLGRADKLTLSHSILAQAKALSHE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.2. A protein with phosphopantothenate--cysteine ligase activity, FMN binding functions is required to enable coenzyme A biosynthetic process, pantothenate catabolic process.3. The designed protein must have at least one Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domain.4. The protein should be designed to have high stability and activity towards H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.5. A protein that can modulate the overall flux of metabolites through the Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MTVIVGITGGIAAYKAVGVVRDLVKRGHDVHVVPTEGALRFVGLPTLEALSRNPVTTSVWDDVAEVRHVALGRRADLVVIAPATADSLARMAHGLASDLLGTTLLATEAPVVVAPAMHPQMWEHPATRANVATLRDRGVHLVGPVVGALTGDDAGIGRMAEPEDIVAAALAAAELALARGPLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHVLGADGSVAEIPHGPKSALAHAVWDTVVRLR",
    "response#2": "MTVIVGITGGIAAYKAVGVVRDLVKRGHDVHVVPTEGALRFVGLPTLEALSRNPVTTSVWDDVAEVRHVALGRRADLVVIAPATADSLARMAHGLASDLLGTTLLATEAPVVVAPAMHPQMWEHPATRANVATLRDRGVHLVGPVVGALTGDDAGIGRMAEPEDIVAAALTAARTVLAGGDRLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHVLFADGSVQELPLLSKREVADRVLDAVLTRLRGA",
    "response#3": "MTVIVGITGGIAAYKAVGVVRDLVKRGHDVHVVPTEGALRFVGLPTLEALSRNPVTTSVWDDVAEVRHVALGRRADLVVIAPATADSLARMAHGLASDLLGTTLLATEAPVVVAPAMHPQMWEHPATRANVATLRDRGVHLVGPVVGALTGDDAGIGRMAEPEDIVAAALALAREALAPPLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHLLAADGSVEELEGVRVVVSAGGTREPLDPVRYLGNRSSGKQGYALARVAAQRGAAVTLVAAHTLALPEPAGVDLVSVSTAEQLGKAVRTAAADADVVVMAAAVADFRPESLAEHKIKKADDGSAPVVTLARNPDILAGLVADRRPGQLIVGFAAETGDERGGVLDHARRKLARKGCDLLVVNAVGEGRAFEVEENTGWLLGADGSAVEVAEGSKAVLATAVWDAVTALRGTN"
  },
  {
    "reference": "MKHKRSDKVAETIHTTISTILSRGLNDPRIGFVTITGVEVTDDLHLARIFFTVIGDEAAKKSSEAGLNSAARFIRHELGKVLKMRYTPDILFKYDHSQEYGQRIDDLLREVGTGNDGNDSEDR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Required for efficient processing of 16S rRNA; May interact with the 5'-terminal helix region of 16S rRNA. The designed protein sequence is ",
    "response#1": "GIVYLGHVPPRFRPLHVRNLLSAYGEVGRVFFQAEDHFVKRKKKAAAAAGGKKGAKYSKDYTEGWVEFRDKRVAKRVAASLHNTPMGARKRSPFRYDLWNLKYLK",
    "response#2": "GIVYLGHVPPRFRPLHVRNLLSAYGEVGRVFFQAEDHFVKRKKKAAAAAGGKKGAKYSKDYTEGWVEFRDKRVAKRVAASLHNTPMGARKRSPFRYDLWNLKYLK",
    "response#3": "MGIVYLGHVPPRFRPLHVRNLLSAYGEVGRVFFQAEDHFVKRKKKAAAAAGGKKGAKYSKDYTEGWVEFRDKRVAKRVAASLHNTPMGARKRSPFRYDLWNLKYLN"
  },
  {
    "reference": "MKAKYVFPSDYMADPAANVFDGKLYIYPSHDYDSGECFDDDGGHFQMKDYHVLCIDGDPMEQDAKDCGKQFGIEDIPWVEKQLWDNDCVEKDGKYYLIYSAKDYTGVFHLGVAVADKPEGPFVPEADPIRGSYSIDPCVFKDDDGEIYVYFGGIWGGQLQWYKDNKMLKAEHLPEGKEDPLPSRVARMTGDVKQFAEAPRAVIIVDETGKPLPADDPHRFFEASWMHKYNGKYYFSYSTGDTHLLCYAVGDNPYGPFTYQGVILEPVVGWTTHHSIVEYKGKWYLFHHDCVPSNDTTWLRSLKVAELEYDAEGHIKTVK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have xylan 1,4-beta-xylosidase activity to facilitate xylan catabolic process.2. The designed protein must have a Proton donor, Proton acceptor that is structurally stable and resistant to denaturation.3. For general function, the protein need meet that Exoxylanase capable of acting on certain xylans and xylooligosaccharides. The designed protein sequence is ",
    "response#1": "GNNFWSLPDAEKYMEVAKREFNILTPGNQMKWDTIHPERNRYNFEPAEKHVEFALKNDMIVHGHTLVWHNQLPGWLTGQEWSKEELLNILEDHVKTVVSHFRGRVKIWDVVNEAVSDSGTYRESIWYRTIGPEYIEKALIWAKEADPDAILIYNDYNIEEINAKSNFVYNMIKNLREKGVPIDGIGFQMHIDYRGINYESFKKNLERFAELGLQIYITEMDRGFPLGGSVGYYLKKQAEVYRRIFEICLDNPAVRAIQFWGFTDKYSWVPGFFKGYGKALIFDENYNPKPCYFAIRELA",
    "response#2": "VGYELWADSGNNSATFYSDGSFSCSFRNAKDYLCRSGLSFDSTKTYQQLGHMYADFKLVKQNIQNVDYSYVGIYGWTRNPLVEFYVVDNWLSQWRPGDWVGNKKHGDFTIDGAKYTVYENTRTGPSIDGNTTFKQYFSIRQQARDCGTIDITAHFEQWEKLGMRMGKMHEAKVLGEAGSTGSGTS",
    "response#3": "MKWEAIQPNRGQFNWGPADQHAAAATSRGYELRCHTLVWHSQLPSWVANGNWNNQTLQAVMRDHINAVMGRYRGKCTHWDVVNEALNEDGTYRDSVFLRVIGEAYIPIAFRMALAADPTTKLYYNDYNLEYGNAKTEGAKRIARLVKSYGLRIDGIGLQAHMTSESTPTQNTPTPSRAKLASVLQGLADLGVDVAYTELDIRMNTPATQQKLQTNADAYARIVGSCMDVKRCVGITVWGISDKYSWVPGTFPGEGSALLWNDNFQKKPSYTSTLNTY"
  },
  {
    "reference": "MRLGRVCPRGPGKVRSPRHRFSCTLFVSTTGSSCGHHGPQLAASSNPSVLPGLHEQPPQASHSRPLNGLLRLGIPGDMYARSEPFAPGPMARSDTLATATALHGYGGMNLTMNLTAPHGPGAFFRYMRQPIKQELICKWLGDDSPMSPRPCSKTFSTMHELVTHVTVEHVGGPEQANHICFWEECPRQGKPFKAKYKLVNHIRVHTGEKPFPCPFPGCGKVFARSENLKIHKRTHTGEKPFRCEFEGCERRFANSSDRKKHSHVHTSDKPYMCKVRGCDKCYTHPSSLRKHMKVHGRSPPPSSGYDSAITSALASPSLESGREPSVACSAAVVVRGTDVSE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.2. The protein must exhibit the following characteristics: Binds to DNA..3. The protein design should be able to enhance the efficiency of central nervous system development, regulation of transcription by RNA polymerase II. The designed protein sequence is ",
    "response#1": "IQEEVALRIISECTTLFRQEKTMLDVEAPVTVCGDIHGQFYDLMKLFEVGGSPASTKYLFLGDYVDRGYFSIECVLYLWALKICYPTTLFLLRGNHECRHLTEYFTFKQECKIKYSEKVYDACMDSFDALPLAALMNQQFLCVHGGLSPEIHTLEDIKKLDRFKEPPAFGPMCDLLWSDPLEDFGSERNSEQFSHNSVRGCSYFYSYAACCDFLQHNNLLSIIRAHEAQDAGYRMYRKSQATGFPSLITIFSAPNYLDVYNNKAAILKYENNVMNIRQFNCSPHPYWLPNFS",
    "response#2": "IQEEVALRIISECTTLFRQEKTMLDVEAPVTVCGDIHGQFYDLMKLFEVGGSPASTKYLFLGDYVDRGYFSIECVLYLWALKICYPTTLFLLRGNHECRHLTEYFTFKQECKIKYSEKVYDACMDSFDALPLAALMNQQFLCVHGGLSPEIHTLEDIKKLDRFKEPPAFGPMCDLLWSDPLEDFGSERNSEQFSHNSVRGCSYFYSYAACCDFLQHNNLLSIIRAHEAQDAGYRMYRKSQATGFPSLITIFSAPNYLDVYNNKAAILKYENNVMNIRQFNCSPHPYWLPNFF",
    "response#3": "IQEEVALRIISECTTLFRQEKTMLDVEAPVTVCGDIHGQFYDLMKLFEVGGSPASTKYLFLGDYVDRGYFSIECVLYLWALKICYPTTLFLLRGNHECRHLTEYFTFKQECKIKYSEKVYDACMDSFDALPLAALMNQQFLCVHGGLSPEIHTLEDIKKLDRFKEPPAFGPMCDLLWSDPLEDFGSERNSEQFSHNSVRGCSYFYSYAACCDFLQHNNLLSIIRAHEAQDAGYRMYRKSQATGFPSLITIFSAPNYLDVYNNKAAILKYENNVMNIRQFNCSPHPYWLPNFV"
  },
  {
    "reference": "MDETSIINENTDVFEKKITLIERNLQEITINDKEIIRKIIRKRNLKIYWGTAITGKPHIGYFLPILKIKDFVEAECEVTILFADIHGFLDNLKAPIELIENRYHYYEKIIKIMLMSVGCDINKIRFVKGSDYQKNSDYVFDLYKLCSYTTERDCKRAGSDVVKQRDNVLLSSLIYPNMQALDEEYLKVDAQFGGEDQRKIFMHAKTFLPKLGYKKRIHLMNPMMPGLNSDKMSSSDELSKIDLLDTEQQINKKINKCFCEEGNLQSGVLHVFEFVIFHYYTSISINKKSYTSIEDVKKDFEMKLIHPKDLKLACSNYINKMVTPIRNEMLKDIDMIKKAYNN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have tyrosine-tRNA ligase activity, ATP binding within cytoplasm to regulate tyrosyl-tRNA aminoacylation.2. The designed protein should contain at least one 'HIGH' region, 'KMSKS' region motif to achieve its intended function.3. The L-tyrosine binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MPTANLSLSKLNEGIGIIDLFAEAGLASTKSDARRLVEQGGAFINEEKISDIKALIGKEKLDKDNEMILRAGKKRFS",
    "response#2": "MPTANLSLSKLNEGIGIIDLFAEAGLASTKSDARRLVEQGGAFINEEKISDIKALIGKEKLDKDNEMILRAGKKRFS",
    "response#3": "MPTANLSLSKLNEGIGIIDLFAEAGLASTKSDARRLVEQGGAFINEEKISDIKALIGKEKLDKDNEMILRAGKKRFC"
  },
  {
    "reference": "MAGNSQRRGAVRKAGTKKGPTVGSGGVRRRGLEGKGATPPAHQRPHHPAGKRAAKAARQAQGRHKKTDDTEIVLGRNPVLECLRAGVPATALYVALGADSDERLTESVQIAADKGIAILEVPRHDLDRIAANGMHQGIALQVPPYNYAHPDDLLAEAKSDAMPALLVALDNISDPRNLGAIVRSVAAFGGHGVLIPQRRSASVTAVAWRTSAGAAARTPVARATNLTRALKQWADAGLQVVGLDADGDTTVDQIDGAGPIVVVVGSEGKGLSRLVRENCDAVVSIPMAGPTESLNASVAAGVVLAEIARQRRSA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must possess RNA binding to support RNA processing under certain conditions.2. Prioritize Basic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "RTFDHVFHLTNVSKLQGTCKKMKLLNQLIDQGGDYLATSLPYVLSILSKGLGPRVALLSHTLPHRPEWDIGEEPAKHRDSSLLSVGLLLEAELHTSVLEKGPAADSSQALDFRAFWGEKSELRRFQDGSICEAVVWPGSSLCEKRKVPELIVKYLLELHK",
    "response#2": "RTFDHVFHLTNVSKLQGTCKKMKLLNQLIDQGGDYLATSLPYVLSILSKGLGPRVALLSHTLPHRPEWDIGEEPAKHRDSSLLSVGLLLEAELHTSVLEKGPAADSSQALDFRAFWGEKSELRRFQDGSICEAVVWPGSSLCEKRKVPELIVKYLLELHR",
    "response#3": "RTFDHVFHLTNVSKLQGTCKKMKLLNQLIDQGGDYLATSLPYVLSILSKGLGPRVALLSHTLPHRPEWDIGEEPAKHRDSSLLSVGLLLEAELHTSVLEKGPAADSSQALDFRAFWGEKSELRRFQDGSICEAVVWPGSSLCEKRKVPELIVKYLLELHK"
  },
  {
    "reference": "MDKMALIKELRERTAAGMSDCKKALEVSNWDVEEAISFLKKNGKIKAASKANRISADGLLVEAGNNERAVLVELNCETDFVAHGEEFVALANTVAQTIVANFELVKENGAEAALALKLANSEEILADAISSYSAKCGEKIELRRFVLIDAGTNQSVSTFVHINGKIGAIMLTEGSDAEAARNVAMHLSAMNPEYIFAEDIPGSVLEKFASEFKEPAGFSDKPEKIQETIRKGFVDKKISEVTLLSQKLIMDESKTVQQYLKELKLRLIKAIRFGLGEGIEKKETDFAAEVAEQMSKSM",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.. The designed protein sequence is ",
    "response#1": "EGGRR",
    "response#2": "LPLL",
    "response#3": "GVLVKVASETDFASRSEVFTGLVQEIADAAAAAAGDGGDIVDIPTFLSNTQSITTGKLLSECLNDAVLSIRENIQLDSIVTIGTTPSSRSVIAGYVHGRAPNSTCGTSAALVEVEVLPKDGGGGDDAVLSEEEKSVAMEAAKKLAMHVVASNPLYLNPESVPVDVVEKEREILMEKMTDSNKPPEIIEKIISGQLRKFYEGICLTEQSHLVEEGNPKISKVMKGLGLVVKDFS"
  },
  {
    "reference": "MRKTKFWLVLSLIATSLSIFACKKDSTATKNPIPEVSKAKASTKLLNATTVATTDYELIWSDEFNSSGGFDSTKWSYADRGTVAWNKYMTSLPAYASQDGSNLVLRMDNAVVAGDPVAYHAGGVKSMGKFSMTYGKVEVRAKFTQGRGSWPAIWMMPEPATAYGGWPSCGEIDSMEHVNNESVMYHTIHNGSVTNANGGSTASKSATYNTTDYNLYTMIWSPNDIRFYVNNSLQYTYARVSGGGTQQWPFDVPFYLILNQAGGAGWPGAITNADLPFSMQVDYVRVYKLPLFSNGDFESGVIYPWTTWGGGSSVVSTDARTGTKCIRETGGETSIEQYLTGLTPNTTYRFGGYAKVSAAGQSVSIGVKNYGGTAVDATIGTTSYSNNSVTFTTGANNTTATVYFYKPLSGTVYGDDFYLEKL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have at least one GH16, CBM-cenC domain.2. A protein that contributes to carbohydrate metabolic process by functioning in extracellular region with keratan-sulfate endo-1,4-beta-galactosidase activity.3. The protein with the reaction Endohydrolysis of (1->4)-beta-D-galactosidic linkages in   keratan sulfate., the Nucleophile, Proton donor must be optimized to ensure efficient catalysis.4. Design the protein with a signal peptide incorporated.5. For general function, the protein need meet that Hydrolyzes internal endo-beta-galactosyl linkages in keratan sulfate and in various neolacto-type glycosphingolipids, producing sulfated and non-sulfated disaccharides, and glucosylceramides respectivly. The designed protein sequence is ",
    "response#1": "GLKVFVYPAVGTISETHRRILASIEGSRFYTFSPAGACLLLLLSLDAQTGECSSMPLQWNRGRNHLVLRLHPAPCPRTFQLGQAMVAEASPTVDSFRPGFDVALPFLPEAHPLRGGAPGQLRQHSPQPGVALLALEEERGGWRTADTGSSACPWDGRCEQDPGPGQTQRQETLPNATFCLISGHRPEAASRFLQALQAGCIPVLLSPRWELPFSEVIDWTKAAIVADERLPLQVLAALQK",
    "response#2": "LDWEDEFNGGNLADRWNFELGCNGWGNNELQCYTDNRGANARQEDGKLVISAVREWWGDGVNPDKEFTSARMTTKANWLHGKFEMRARLPKGKHLWPAFWMMPQNSEYGGWPRSGEIDITEYRGQRPQQILGTLHFGAAPDNKGDVGTGERDFPIDFSADFHTFGLDWSPDSMQWLLDDQVYHTESLQRNFWDGVYNQNGSPFDKNFFIILNLAVGGNFFGGEPFDPSESDGWAKNTFEVEYVKKWTWK",
    "response#3": "VGNNTCFYGKCYYCRETEPACADGDMMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCDAVKKTSPYDSGPRLLDIIDTAVFDYLIGNADRHHYESFQDDEGASMLILLDNAKSFGNPSLDERSILAPLYQCCIIRVSTWNRLNYLKNGVLKSALKSAMAHDPISPVLSDPHLDTVDQRLLNVLATIKQCTDQFGTDTVGNNTCFYGKCYYCRETEPACADGDMMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCDAVKKTSPYDSGPRLLDIIDTAVFDYLIGNADRHHYESFQDDEGASMLILLDNAKSFGNPSLDERSILAPLYQCCIIRVSTWNRLNYLKNGVLKSALKSAMAHDPISPVLSDPHLDTVDQRLLNVLATIKQCTDQFGTDTGNKKADDNYVVTWGQNNVLKLNQGKEVQLSLDHSSGSGFESKNHYESGFFQIRIKVPPKDTSGVVTAFYLTSKGNTHDEVDFEFLGNKEGKLAVQTNVFTNGKGNREQKLALWFDPSKDFHTYAILWNPYQIVLYVDNIPVRVFKNTTSQGMNYPSKPMQVVVSLWNGENWATDGGKSKINWVGNNTCFYGKCYYCRETEPACADGDMMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCDAVKKTSPYDSGPRLLDIIDTAVFDYLIGNADRHHYESFQDDEGASMLILLDNAKSFGNPSLDERSILAPLYQCCIIRVSTWNRLNYLKNGVLKSALKSAMAHDPISPVLSDPHLDTVDQRLLNVLATIKQCTDQFGTDT"
  },
  {
    "reference": "MNQYKNFIMQFEDIVGNNNVLIDEPMKKHTSFKVGGPADLLITPTTLEQVKDSIILCRNNSIPYYIIGNGSNLLVRDGGIRGVVIKFLKLGDIKVEGDRVIAQSGAPLTNICNEALKSNLGGLEFACGIPGSVGGAVTMNAGAYNGEISQVIESAKVIDKDGNVFLLNKEQLDLGYRMSAIQKYHYIVLEVTFKLHNSEYDTIKNRIMDLNRRRTEKQPLEYPSAGSTFKRPEGHFAAKLIEDTGLKGESIGGAQVSEKHSGFIINKGGATAGDILNLIEFVQNKVMEKFEVDLHTEVRIIGEENN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that can modulate the overall flux of metabolites through the Cell wall biogenesis pathway, enhancing or suppressing its activity as needed.2. The designed protein should be optimized for flavin adenine dinucleotide binding activity in order to improve cell wall organization, regulation of cell shape, peptidoglycan biosynthetic process.3. The protein should be designed to catalyze the following reaction NADP(+) + UDP-N-acetyl-alpha-D-muramate = H(+) + NADPH + UDP-   N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine.4. The protein contains novel FAD-binding PCMH-type domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "VGGPAEAMFKPADAQDLADFLAGRPRDVAVRVIGVASNLLVRDGGVPGVVIRLGRAFTGVEVVGETLVCGASALDATVAKVAEAAGLAGLEFLSGIPGTLGGALRMNAGAHLREMADIVVLATAVDGLGQSHTLTPAQMGFSYRACALPEDWIFTGCVLAGRPGQVADLSAWAWQEIDAHLQESQKRRRSTQPIAKSAGCIFKNPDSCPAGKLVDELGLKNMRIGQARVSEVHGNFIVNDGGATAADMLALIERIQTAARTKRGLELETEVR",
    "response#2": "VGGPAQFWVEPRSENSFAELIRFCRRESLPLFVIGRGSNLLVRDGGIRGVVVHPSGGEFDKIDIDGQEITAGVGVGMKEVAYAARSAGLGGLEFMEGIPGAVGGGLRMNAGAMGSQLFEKVARVHYLDAEGNPHTQDRHELEVRYRDFPLLRNNYAVSATFRGAARKVAVCGMHGKTTTTAMAAHVLRLGGLKPCHYVGADIPILGANARWDSESEYFVVEGDESDGSLTTYFPEHALVLNVEPEHLDFYQDQDAIDDVFSRLIAQTSGWTFYCADDAGARRVCTSHPRTVCYGTNASCDYWMELFSSSRNGGILTQLAIYHRKSFLGNIQLNVPGRHNALNALAVTALAWELGLSLADIAKSLSIKSKLIDFSTRRWEKQPIDLPSAGSIFKRPKPDFFVGTTIENLGLKGFSIGEAQISTKHAGFIINKGNATFKDVISLIEYVKRIVKDKYNVDLQVEPESKSILQHRLMTQPYTQPSAGCIFRNPEGTSAGKLIDAAGLKGLAIGGAQISPLHANFIINTGKATSDEVKQLIAIIQSTLKTQGIDLEHEIRIIGC",
    "response#3": "VGGPAQFWVEPRSENSFAELIRFCRRESLPLFVIGRGSNLLVRDGGIRGVVVHPSGGEFDKIDIDGQEITAGVGVGMKEVAYAARSAGLGGLEFMEGIPGAVGGGLRMNAGAMGSQLFEKVARVHYLDAEGNPHTQDRHELEVRYRDFPLLRNNYAVSATFRGAAEHSIVGDVCGMHGKTTTTAMAAHVLRLGGLKPCHYVGADIPILGANARWDSESEYFVVEGDESDGSLTTYFPEHALVLNVEPEHLDFYQDQDAIDDVFSRLIAQTSGWTFYCADDAGARRVCTSHPRTVCYGTNASCDYWMELFSSSRNGGILTQLAIYHRKSFLGNIQLNVPGRHNALNALAVTALALECGVDEQTAALGLAGLTGAPRRFEVKFKGESNMVVDDYAHHPTEVKATLAAAKNSGWKRVIALFQPHRYSRTKLLLNDFAEAFHDAHEICLTEIYAASEKPLDGISGERLAEVVRESGHKAVFFEPRLDRLRSLVSRSLQDGDLILTMGAVSRAICSIRSEKLPDPAVLGNAGSFFKNPLVSAAVVAQIKAQHPDLVAYAQPDGQMKLAAGWLIERAGWKGFREADAGVHKLQALVLVNYGAATGLQLLDLAQRIQKDIAERFNVELEMEPGADLRAAIDDLL"
  },
  {
    "reference": "MICVSIGRTRHEMMIAEQHALFERRAKLLELRIDWLKKSPDLPRLLKDRPVPTVVTCRRPADQGRWAGSEEQRLGLLRQAIIEGAEYVDLEEDAAKRIPRHGDTQRIVSYHDFSETPDNIDEIHRRLADCDADVVKIVTTALHPSDNVRMLNLVRRASIPTVGFCMGELGLLSRVLCGRYGAPFTYASFSQDRVMAPGQVSFDQMQHEYFYERITAKTQLFGVMGDPIGHSKSPRLHNQLLRDSRLDAVYLPLLVPKGSFASAFADYATLGFDGFSVTIPHKEEAVRAAARADDDVMQIGASNTLYRDGSQWVAANTDYTAALSSLVDAIRETDPDATVSGKRAIILGSGGVARAVAVGLIRSGALVTITGRTRPKAEKLAAEIGCHIVSWENRSSETYEVVVNCTPVGMHPNVDETPFPDNAFSEHGVAFDTIYTPENTLFLKQARSRGCLTVSGLEMFVRQAAAQFELFTGLPASLPLLRRDLRREISAARLPS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of Pyrroline-5-carboxylate reductase catalytic N-terminal, Shikimate dehydrogenase substrate binding N-terminal, SDH C-terminal domains is critical for the interaction of the designed protein with its intended targets.2. The protein must exhibit the following characteristics: Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids.3. The protein design should take into account the physiological conditions of the chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7 pathway.4. The protein design must be able to perform 3-dehydroquinate dehydratase activity, NADP binding in order to contribute to shikimate metabolic process, aromatic amino acid family biosynthetic process.5. The Proton donor/acceptor, Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "GMAEVIKTAAIWSESDFTLLENGAESIRDAVLKPDLSVPFQGATESTRTPAQTLLLTVVLASAAVKAHVVTNDERESDLRGLLNFGHSVGHAIEAIVSPMMLHGECVSIGMIKEAEIARYLGHLSQVAVGRLTRCLQSYGLPISLEEKRVKQWIGDKHCHVDEMLEIMKVDKKGAEVIVDLLEAVGVLGTQRVMVLGAGGAARAAALACRLKGADVTIANRTYARAHALALASDCRAVELSEAPRRAYDVLINGTSVGMFPDVDESPIPESVLGAGGVGKAIVFGLIRRKAKVVLADGLPERARGLAERFDCRCIEWSLRHTVGPDVLVNCTPVGMHPNVDLAPEDVKRGTYVFDLIYNPLETKLLRLARERGCKTIPGLDMLAEQAVRQFEWWFGLRPSYELFRRVLRERIRD",
    "response#2": "MSLRGAKVLVLGAGGAARAAVFGLRDKGSEVFILNRTPETAQKLARQAGAKTIKKDALAKTNFDVIVNATSVNTEIAEKVVAAGKNYVDVRVAVLDSAVEKARAAAAAGITVVTGCGATPGLLTAAAALAAQSFASVHEVEIAWGLGYETWHGATVKRDIAEMRGKGYKITDWHALRDDEILDMMEITDGVFSLEDVEKHTRCPVGGIKVRTMERSKEEAKKMGLKPGIVTVTVKGHRDHTKTVTLHGAQDIFGTSAAAAVNGYLKALEIVLGAGGVGKAIVFGLIRRKAKVVLADGLPERARGLAERFDCRCIEWSLRHTVGPDVLVNCTPVGMHPNVDLGMAEVIKTAAIWSESDFTLLENGAESIRDAVLKPDLSVPFQGATESTRTPAQTLLLTVVLASAAVKAHVVTNDERESDLRGLLNFGHSVGHAIEAIVSPMMLHGECVSIGMIKEAEIARYLGHLSQVAVGRLTRCLQSYGLPISLEEKRVKQWIGDKHCHVDEMLEIMKVDKKLGLDVTVREVLGNPVSKSMGPWIHNRAFAEMDWPGIYLPFYADNAEKFFKAFESEIEGLSITMPFKEDMARLAGTVEDAAKKIGAVNTVRVGEGEVKGYNTDWKAMVATEAVGVLGTQRVMVLGAGGAARAAALACRLKGADVTIANRTYARAHALALASDCRAVELSEAPRRAYDVLINGTSVGMYPNVDETPVGRELLNDLVVVADVV",
    "response#3": "GMAEVIKTAAIWSESDFTLLENGAESIRDAVLKPDLSVPFQGATESTRTPAQTLLLTVVLASAAVKAHVVTNDERESDLRGLLNFGHSVGHAIEAIVSPMMLHGECVSIGMIKEAEIARYLGHLSQVAVGRLTRCLQSYGLPISLEEKRVKQWIGDKHCHVDEMLEIMKVDKKGAKLAVVDLGMAEVIKTAAIWSESDFTLLENGAESIRDAVLKPDLSVPFQGATESTRTPAQTLLLTVVLASAAVKAHVVTNDERESDLRGLLNFGHSVGHAIEAIVSPMMLHGECVSIGMIKEAEIARYLGHLSQVAVGRLTRCLQSYGLPISLEEKRVKQWIGDKHCHVDEMLEIMKVDKKGTTSLVKL"
  },
  {
    "reference": "MLKMEVVLFTFLVLFPLSTLQLETDQPVERYVENKQDLNPDESRNFMLPIVKKCCTACRMPPCKCCA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must contain a signal peptide for proper functionality.2. The protein can be expressed in extracellular region and perform toxin activity without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "GFVSPL",
    "response#2": "MPIGVPKVPFRSPG",
    "response#3": "LMATRR"
  },
  {
    "reference": "MRYSRQLLLPGLGVKGQMSLLNTSVLVVGCGGLGCPLALYLAAAGIGRLGLLDYDGVELSNLHRQVLHTERTQGLPKAQSAARALSRLNSTTQCVPYHLQLSSENALQLIQQYDIVADCSDNVPTRYLVNDACVLSSKPLVSASALRMEGQLTVYNYNGGPCYRCLYPVPPPPETVTNCSDGGVLGVVVGVMGCLQALEVLKIASGQKSSFGQQLLMFDAQDGRFRCIKLRAKQAGCVVCGETPTVTELQDYESFCGSSATDKCRRLNLLSAEQRVSVQEYKSIVDCGTPHLLLDVRPKVEVDICHLPVSINIPLASLEDQKGDHIHFLKEKISELKLQMSSELEVPVYVVCKLGNDSQTAVQLLEMMSGTELENITVKDITRGLMAWANKIDPSFPKY",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should interact with enzymes and substrates within the tRNA modification, 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis pathway to enhance or regulate its activity.2. For general function, the protein need meet that Also essential during biosynthesis of the molybdenum cofactor; Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A; Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.; Subsequently, a transient disulfide bond is formed; The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A; Its N-terminus first activates URM1 and MOCS2A as acyl- adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (- COSH) of their C-terminus3. A protein that contributes to Mo-molybdopterin cofactor biosynthetic process, tRNA wobble position uridine thiolation by functioning in membrane with ATP binding, metal ion binding. The designed protein sequence is ",
    "response#1": "MVLYSRQLVLPELGVRGQLRLAGSSVLVVGCGGLGCPLAQYLAAAGVGRLGLVDHDVVELNNLHRQVLHRESRLGSSKAQSAASALRELNSTVECVPYEACLSPSNALQLVRAYDIVADCSDNVPTRYLVNDACVLAGKPLVSASALRFEGQLVVYNYLGGPCYRCLFPKPPPPETVTNCADGGVLGVVPGILGSLQALEVLKIASGIGTGLNRAMLLFDALEGKFRQIKLRSRNLDCAVCGDNPSITLKDLNGEAHVLVDVRPEHHFQIVSLPKSLNIPLPKLEGKLPEISSALREEQEHTGVISCSDASLYVICRRGNDSQRAVQYLHKMGFTSAQDIIGGLESWAHDVDPNFP",
    "response#2": "HSRHLLLPSFGVQGQANLSKSSILVVGAGGLGSPALLYLAACGVGRLGIVDHDIVELNNLHRQIIHTEAYIGQPKVTSAAAACRAINSTVQIVEHKEALRTSNALEIVNEYPLYDIVIDATDNLPSRYMISDCCVVSGKPLVSGAALGLEGQLTVYNHNGGPCYRCLFPTPPPTSACQRCSDSGVLGVVPGVIGCLQALEAIKIASVVGEPLSGRMLLFDALSARIRVVKIRARSLQCYSRHLVLPSFGVQGQANLLKSSILVVGAGGLGAPALLYFAASGVGRLGVIDHDVVELNNMHRQVIHTEAYVGKPKVKSAAAACRSINSTIQVVEHEEALRTSNALELLSKYDIIVDATDNAPTRYLISDCCVVLGKPLVSGAALGLEGQLTVYNYNGGPCYRCLFPTPPPRTACQSCAEGGVLGVVPGIIGCLQALEAIKIAASVGEPLSGRMLILDALSGRIRIVKIRGRSMQCSACGSNVQISSLEKYARQLILPDFGVSSQLRLRSTSVLVIGAGGLGCPAAAYLAGAGVGTLGIIDGDVVEASNLHRQVGHGTARIGALKAPSLAAHCRALNPLVRYVSHAEHLTARNAADVVGRYDVVLDCTDRPSSRYLVSDACVLLGKPLVSASALRMDGQLVVLNYPPSRRGRRSGGGGGQAEEDRETGRKDNEKDEEIGGPCYRCLFPRPPPAESVLSCGEGGILGPVVGVMGVLQALEAIKLIIASPSPYPSCSACSPGVALVTTYASGATHVLVDVRDKEHFSLSHIPGSINVPIGRFTSHRGGDTLPEGIPADLPADVPIYVVCRVGNDSQIAAAKLKELGLDRNGHRFVGDILGGLRSWKDSVDDSL",
    "response#3": "YGRQLILSGIGLQGQLRLKNASVLVIGVGGLGCPAAAYLVGAGVGTIGLVDGDIVEESNLHRQILHSTSRVGMSKVDSARVFLSSLNPNVAVVPHISRLAPESAISVVESYDMVLDCTDTPASRYLISDTCVLLGKPLISASALKLEGQLMTLNNPPLPPGDSRGGPCYRCIFPKPPPPESVLSCGDGGILGPVVGVMGVLQALEAIKVLTKDPGTLAPEHVNPEPPTLLIFNGYGNPMFRSIRLRTRKPRCYGRQLILSGIGLQGQLRLKNASVLVIGVGGLGCPAAAYLVGAGVGTIGLVDGDIVEESNLHRQILHSTSRVGMSKVDSARVFLSSLNPNVAVVPHISRLAPESAISVVESYDMVLDCTDTPASRYLISDTCVLLGKPLISASALKLEGQLMTLNNPPLPPGDSRGGPCYRCIFPKPPPPESVLSCGDGGILGPVVGVMGVLQALEAIKVLTKDPGTLAPEHVNPEPPTLLIFNGYGNPMFRSIRLRTRKPRCSACGENAAVTLQDLRYGRQLIIPNVGVLGQLRLKESRILIIGAGGLGCPAAAYIAGAGAGTVGLVDGDVVEVSNLHRQVAHSTDRVGMPKVESAIAYLKGLNPLVTYRAHKEHLSPLNAEDIVSQYDIVLDCTDHPTSRYLISDICILLQKPLVSASAFRTDGQLIILNSPAAPQGSLEGGPCYRCVFPKPPPPDSVVSCGEGGILGPVVGVMGVLQALEAIKLVAAGAVDPKVEKKEAAAPTLLIFSGAAPSGPFRSVRMRGRRKD"
  },
  {
    "reference": "MVAGTRCLLALLLPQVLLGGAAGLVPELGRRKFAASSSGRPSSQPSDEVLSEFELRLLSMFGLKQRPTPSRDAVVPPYMLDLYRRQSGQPGSPAPDHRLERAASRANTVRSFHHEESLEGLPRMKFITYPPKRQTVFHQRINIYEMQKPATANSKFPVTRLLDPRLAGMPECKQVGSFDVTPACDREHRQPWIHVVEVTHLEEKQGVFGRDHAGLCSQDEHSWSQIRPLGQSSSPAKEKRQANTNSGSRLKSSVRDTFLTWVDDLDCGSPGTVSAPFLPAENALFLPDCLRPCCVPTELSVVFKNYQGGRGHGCGRVVGCR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that contributes to thyroid-stimulating hormone-secreting cell differentiation, astrocyte differentiation by functioning in extracellular space with phosphatase activator activity.2. The protein contains novel TGF-beta propeptide domains that confer a unique function or activity.3. The protein design should emphasize Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "STCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGDVPPGPLPEAILALYNSTRDRVAGESAETEPEPEADYYAKEVTRVLMVEYGNKIYDKMKSSSHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLTRREEIEGFRLSAHCSCDSKDNTLQVDINGFSSGRRGDLATIHGMNRPFLLLC",
    "response#2": "STCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGDVPPGPLPEAILALYNSTRDRVAGESAETEPEPEADYYAKEVTRVLMVEYGNKIYDKMKSSSHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLTRREEIEGFRLSAHCSCDSKDNTLQVDINGFSSGRRGDLATIHGMNRPFLLLC",
    "response#3": "RAFQTVVLDPEGDAQIDPNWVVLNQGREIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTVTDDDYAGFIFGYQDSSSFYVVMWKQMEQTYWXXXXXXXXXTYWQANPFRAVAEPGIQLKAVKSSTGPGEQLRNALWHTGDTESQVRLLWKDPRNVGWKDKKSYRWFLQHRPQVGYIRVRFYEGPELVADSNVVLDTTMRGGRLGVFCFSQENIIWANLRYRCNDTIC"
  },
  {
    "reference": "MIVTLNPKILHFSKIHPFSRPSSYLCRTRNVSLITNCKLQKPQDGNQRSSSNRNLTKTISLSDSAPPVTEETGDGIVKGGGNGGGGGGDGRGGLGFLKILPRKVLSVLSNLPLAITEMFTIAALMALGTVIEQGETPDFYFQKYPEDNPVLGFFTWRWISTLGLDHMYSAPIFLGMLVLLAASLMACTYTTQIPLVKVARRWSFMKSDEAIKKQEFADTLPRASIQDLGMILMGDGFEVFMKGPSLYAFKGLAGRFAPIGVHIAMLLIMVGGTLSATGSFRGSVTVPQGLNFVMGDVLAPIGFFSIPTDAFNTEVHVNRFTMDYYDSGEVSQFHSDLSLRDLNGKEVLRKTISVNDPLRYGGVTVYQTDWSFSALQVTKDGEGPFNLAMAPIKINGDKKLYGTFLPVGDTNAPNVKGISMLARDLQSIVVYDLDGKFAGIRRPSSKLPIEINGMKIVIEDAIGSTGLELKTDPGVPVVYAGFGALMLTTCISYLSHSQIWALQNGTALVVGGKTNRAKNQFPDDMNRLLDQVPELIKKNTSVVSEQS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must be able to regulate cytochrome complex assembly. The designed protein sequence is ",
    "response#1": "LSNLYESLIFLAWAFSIIHMVSYFNKKKKKNLNAITAPSAIFIQGFAASGLLNMPQSAILVPALQSQWLMMHVSMMILGYGALLCGSLLSIALLVITFRKVGPTFLKKNLKKKFLLNELFSFDVLYYINEKNSILLQQNINFSFSRNYYRYQLIEQLDFLSFRIISLGFIFLTIGILSGAVWANETWGSYWNWDPKETWAFITWTIFAIYLHIKTNRNVKGINSAIVAAIGFLLIWICYFGVNLLGIGLF",
    "response#2": "LSNLYESLIFLSWSFSIIHMVPYFKKYKNYLKAITAPSAIFTQGFATSGLLTEMHQSEILVPALQSHWLMMHVSMMVLGYAALLCGSLLSLALLVITFRKFISIFSKSNNFVNESFSLGEIQYVREKNNLLPNTSFLSSRNYYRFQLIQQLDLWSYRIISLGFIFLTIGILSGAVWANEAWGSYWNWDPKETWAFITWTLFSIYLHTRTNKNFESVNSAIVASMGFLIIWICYFGVNLLGIGLM",
    "response#3": "LSDLYESLMFLSWSFSIIHMVPKIWNHKNDLSAITAPSAIFTQGFATSGLSTEMHQSAILVPALQSQWLMMHVSMMLLSYAALLCGSLLSVALLVITFRKNIGIVGKSNHLLIGSFSFDEIHYLNEKRNVLQNTSFLSFRNYHRYQLTQRLDYWSYRVISLGFTFLTIGILSGAVWANEAWGSYWNWDPKETWAFITWTIFAIYLHTRTNRSLQGVNSAIVASMGFLIIWICYFGVNLLGIGLV"
  },
  {
    "reference": "MSATPLVSQVNSPNFGKSELIGPLLGASVVELTAWVQEQGQPAYRGKQLHDWIYDKGVRSLGDISVFPKSWREKVADVPIGRSSLHYRAVAPDDTVKYLLKLADGEIIETVGIPSHKRLTVCVSTQVGCPMACDFCATGKGGYKRNLSRGEIVDQVLTVQEDFQQRVSNVVFMGLGEPLLNTENVILALKALNEDVGIGQRSLTISTVGIRDRIHQLAQHHLQITLAVSLHAPNQALREQLIPSAKPYPIEDLLNECREYVEITGRRVTFEYILLAGVNDLPEQALELSKRLRGFQSHVNLIPYNPIEEVDYKRPNRDRIQAFVNVLQQQNITVSVRYSRGLEADAACGQLRTSKR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein's Proton acceptor, S-methylcysteine intermediate must be carefully engineered to ensure that the amino acids involved in adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine are properly positioned for optimal catalysis.2. The protein contains novel Radical SAM core domains that confer a unique function or activity.3. The designed protein should have rRNA (adenine-C2-)-methyltransferase activity, tRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding, rRNA binding within cytoplasm to regulate rRNA base methylation.4. The protein should have a high affinity for [4Fe-4S] cluster, S-adenosyl-L-methionine.5. The protein must be engineered to have a high affinity for [4Fe-4S] cluster in order to increase its catalytic activity.6. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. The designed protein sequence is ",
    "response#1": "QAGRGTLCVSSQAGCALDCSFCSTGKQGFNSDLTSAEIIGQVWIANKSFGTVPGKIDRAITNVVMMGMGEPLLNFDNVVTAMSIMMEDFGYGISKRKVTLSTSGVVPMIDKLGETTDVSLALSLHAPNDELRNQLVPINKKYPLKMLLDACRRYITRLGKERVLTIEYTLLSNVNDQPEHAEQMIALLKDFPCKINLIPFNPFPHSGYERPSNNSIRRFQDMLHKGGFNVTVRTTRGDDIERSRAGGGAVGPTTRAALKTMDRAELEAFAQSLGEPAFRGRQLYTWLVEKGATTFEEMTTLPKALRERLSKIATLGTIEEVRRQTATDKTVKSLFRLPSGRHVEAVLIPDFDDDDRAKRLTVCVSSQVGCAMGCHFCATGLMGFHENLTAGQILDQVRAMDTLARERFGRGITNVVYMGMGEPLQNYANVFKSTQFIRDGFDLSPKRITVSTVGLARRIRQLADDDAPFGLAISLHAPTDPQRSAIMPVNRSEKTDLAALKDAVQHYHATTGKRVTYEYCMFADMNDSAADAKNLATVARWAPSKVNLIMYNPVEGTRFTSTDEARLNRFIRVLVDEGVRVTVRRSRGQDIDAACGQLAVQRVP",
    "response#2": "EGLGEPSYRGGQILEWLWQKRATSIEGMSNLPVRLREHLEESFTLRSLSPAKSQGSRDSTRKFLFRLNDGRYIECVLIPASPALYGERSDRRTLCVSSQVGCAFGCKFCASGLDGFTRNLTADEIAGQVLLAEELSGEKVNNIVFMGMGEPLANLPQLLKALELITSPWGLGIGARHLTVSTSGLVPQIRKLADHPQQIRLAISLHGASNDVRDRIMPVNRKWPVEDLFKALSYWTKRKNQKITLEYILIDGINADPAQARLLAQHAESLRAKVNLIPYNTVDGLKWKRPPEEECHAFRDILAARGIPATLRLEKGHDIDAACGQLRLKEERAEKRRRGE",
    "response#3": "MAKANLLGMTKPQLEEFFESIGEKRFRAGQVMQWIHHFGAEDFDAMTNVGKALREKLKACAEIRGPEVVSQDISSDGTRKWVVRVASGSCVETVYIPQAGRGTLCVSSQAGCALDCSFCSTGKQGFNSDLTSAEIIGQVWIANKSFGTVPGKIDRAITNVVMMGMGEPLLNFDNVVTAMSIMMEDFGYGISKRKVTLSTSGVVPMIDKLGETTDVSLALSLHAPNDELRNQLVPINKKYPLKMLLDACRRYITRLGKERVLTIEYTLLSNVNDQPEHAEQMIALLKDFPCKINLIPFNPFPHSGYERPSNNSIRRFQDMLHKGGFNVTVRTTRGDDIDAACGQLVGQVMDRTRRSRQEAQMRGV"
  },
  {
    "reference": "MLSSLKDFFVLLLPFFIGIAFIYKLWNFTSKKNLPPSPRRLPIIGNLHQLSKFPQRSLRTLSEKYGPVMLLHFGSKPVLVISSAEAAKEVMKINDVSFADRPKWYAAGRVLYEFKDMTFSPYGEYWRQARSICVLQLLSNKRVQSFKGIREEEIRAMLEKINQASNNSSIINGDEIFSTLTNDIIGRSAFGRKFSEEESGSKLRKVLQDLPPLLGSFNVGDFIPWLSWVNYLNGFEKKLNQVSKDCDQYLEQVIDDTRKRDEENGANNNGGNHGNFVSVLLHLQKEDVKGFPSEKGFLKAIILDMIVGGTDTTHLLLHWVITELLKNKHVMTKLQKEVREIVGRKWEITDEDKEKMKYLHAVIKEALRLHPSLPLLVPRVAREDINLMGYRVAKGTEVIINAWAIARDPSYWDEAEEFKPERFLSNNFDFKGLNFEYIPFGSGRRSCPGSSFAIPIVEHTVAHLMHKFNIELPNGVSAEDFDPTDAVGLVSHDQNPLSFVATPVTIF",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that localizes to endoplasmic reticulum membrane and can perform iron ion binding, oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, monooxygenase activity with high specificity and efficiency.2. A protein that can modulate the overall flux of metabolites through the Alkaloid biosynthesis pathway, enhancing or suppressing its activity as needed.3. The protein must exhibit the following characteristics: Component of the monoterpenoid indole alkaloids (MIAs, e.g.4. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "TYNEMDRLSSHLAQRLRDAGVEPGVFVALCLDRCKWAVIGIVAVMKAGGAFCALDPSYPVSRLKEMCRDLGITIVLTVKSNIQHASPLASKVFALDDDVYFESALSSAHESASWVSVSPHDPVYAVFTSGSTGKPKGIIMEHASFSACALSSVKPLQIADQDRVLHFASYAFDASVIEILAPLIAGATVAIPSERARLEDLPRAMTDLKATWAFLTPTVARLYRPEQMPTLKTLCLGGEAVNASDTRSWSSKNLISGYNPAECCPLGISGPLNDRMPRSLGSTFASQTAWIVDPKDHEKLLPAGAIGELAIEGPVVARGYIHDVTCSDPSTPFVVKLPPWLRRFRATANRGNRIYLTGDLARLDCDDGSVHYLGRKDDQVKIHGQRVELAEIEHHLEQM",
    "response#2": "MGNSGEKCPVMSSSTQSRGPRDIYTLEALSHFNREKIPERAVHAKGTGAYGEFEVTADISDICNIDMLLGVGKKTQCVTRFSTTGLERGSSDGVRDLKGMAVKFFTEQGDWDWVSLNFPFFFIRDPAKFPDMIHSQRRDPQTNLLNPNMTWDFVTKNPEALHMTLLQHSDFGTMFTWRTLSSYVGHAFKWVMPDGSFKYVHFFLASDRGPNFTDGSTAKVDPNDPDFATKDLFEAIERGDYPSWTANVQVVDPKDAPKLGFNILDLTKHWNLGTYPKGLDTIPSRPFGKLTLNRNVKDYFSEVEKLAFSPSNLVPGVEPSEDPILQARMFAYPDAQRYRLGIDHLKAPLRRKETACQHDLGPEFEKWLSQVTSEAWSHPHEL",
    "response#3": "MGNSGEKCPVMSSSTQSRGPRDIYTLEALSHFNREKIPERAVHAKGTGAYGEFEVTADISDICNIDMLLGVGKKTQCVTRFSTTGLERGSSDGVRDLKGMAVKFFTEQGDWDWVSLNFPFFFIRDPAKFPDMIHSQRRDPQTNLLNPNMTWDFVTKNPEALHMTLLQHSDFGTMFTWRTLSSYVGHAFKWVMPDGSFKYVHFFLASDRGPNFTDGSTAKVDPNDPDFATKDLFEAIERGDYPSWTANVQVVDPKDAPKLGFNILDLTKHWNLGTYPKGLDTIPSRPFGKLTLNRNVKDYFSEVEKLAFSPSNLVPGVEPSEDPILQARMFAYPDAQRYRLGIDHLKAPLRRKETACQHDLGPEFEKWLSQVTSEAWSHPHEL"
  },
  {
    "reference": "MKITVNFLLVALIGVPSVLSDRHITRDKRQAMRDYAKWENNKMSLFFYNLPLEMQAMFRDAINYLENHTCLKFEYNENAETAVRIRKGNGCYSLYGMHAGEVQDLTLDYNCASFGTAVHEIMHALGIAHGQARSDRDDYLIVDSTNSNDGIENTENLVPFDYGSVMLYARDPHSDKRIPIDPEYNFTMGSLRVAFYDMVLLNKFYGCNCDNHPRKLDCKNGGYQNPANCEECLCTDGFNGQLCDQHEGVYVLEAKKEWDASGVRNNYRKGIETNTMPEYTYFALTAPEGSTIEVRITKLSGFFCQHTCDYNGVELKYKTDRRIVSPLVCCDNDNLWNKTRSSTNNPFIIAKYGNNRTPHFEFEYRYIPGNATAAPEENN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein have Peptidase M12A domains with specific sequence motifs that are crucial for its activity.2. The active site active site of the designed protein must be optimized for substrate binding and specificity.3. The protein design must be focused on enhancing the efficiency of molting cycle, collagen and cuticulin-based cuticle.4. The protein must bind to Zn(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "VVGGRVAQPNSWPWQISLQYKSGSSYYHTCGGSLIRQGWVMTAAHCVDSARTWRVVLGEHNLNTNEGKEQIMTVNSVFIHSGWNSDDVAGGYDIALLRLNTQASLNSAVQLAALPPSNQILPNNNPCYITGWGKTSTGGPLSDSLKQAWLPSVDHATCSSSGWWGSTVKTTMVCAGGGANSGCNGDSGGPLNCQVNGSYYVHGVTSFVSSSGCNASKKPTVFTRVSAYISWIHAN",
    "response#2": "VVGGRVAQPNSWPWQISLQYKSGSSYYHTCGGSLIRQGWVMTAAHCVDSARTWRVVLGEHNLNTNEGKEQIMTVNSVFIHSGWNSDDVAGGYDIALLRLNTQASLNSAVQLAALPPSNQILPNNNPCYITGWGKTSTGGPLSDSLKQAWLPSVDHATCSSSGWWGSTVKTTMVCAGGGANSGCNGDSGGPLNCQVNGSYYVHGVTSFVSSSGCNASKKPTVFTRVSAYISWIR",
    "response#3": "SSPLDSIYYHRELLCNSLDGLRVDLLTVSSCHGLRDEREPRLEQLFPDTSTPRPFCFSGKRIFFLSSRVHPGETPSSFVFNGFLDFILRPDDPRAQTLRRLFVFKLIPMLNPDGVVRGHYRTDSRGVNLNRQYLKPDAVLHPAIYGAKAVLLYHHVHSRLNPHTPSDQQPNPPPSAQPGPLSELEKTDNLNNEADLGLSTDGENSESWTQTEPANQDPNSLWFLESESGEVEPAPETIPPKESGVAYYVDLHGHASKRGCFMYGNSFSDESAQVENMLYPKLISLNSAHFDFQGCNFSEKNMYARDRRDGQSKEGSGRVAIYKASGIIHSYTLECNYNTGRSVNSIPAACHDNGRASPPPPPAFRSRYTVELFEQVGRAVAIAALDMAECNRTQPGVIYDIHHQCQLQYGPNASFCQEVDNVCQTLWCSVKGYCRSKLDAAADGTRCGEKKWCMAGECQPGVIYDIHHQCQLQYGPNASFCQEVDNVCQTLWCSVKGYCRSKLDAAADGTRCGEKKWCMAGECA"
  },
  {
    "reference": "MSITIPYDGSISREPSPSPPPPKANTKNLPTKILSNFLIGLIMIPVAITAFIFILTSLGFTFFFAFYWFLQRNYRHRLRRHRRHEYSDGLSPRCVKRLPQFKYCEPSSEYGGDDCVVCIDGFRQGQWCRKLPRCGHVFHRKCVDLWLIKVSTCPICRDRVYRFEEGRRWRPQGEIF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. We require a protein that can specifically bind to the metabolites produced in the Protein modification pathway.2. A protein with metal ion binding, transferase activity functions is required to enable protein ubiquitination.3. The protein should have Helical domains to ensure proper insertion into the membrane.4. The desired protein should be able to perform S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +   [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-   cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. The designed protein sequence is ",
    "response#1": "MKFGAIYEEYLREQQDKYLTKCSHVEYKRLKKVLKKCRVGRSLQEDCPNGDQQEGNNESPDICKCNSCTLCDQMFFTELTKEASEIAGCFSSRVQRLLNLHVPSGFLRYIWRVRQCFIDDQQIMVQEGRMLLNYVTMNAIAIRKILKKYDKIHGSVSGRDFKL",
    "response#2": "MKFGAIYEEYLREQQDKYLTKCSHVEYKRLKKVLKKCRVGRSLQEDCPNGDQQEGNNESPDICKCNSCTLCDQMFFTELTKEASEIAGCFSSRVQRLLNLHVPSGFLRYIWRVRQCFIDDQQIMVQEGRMLLNYVTMNAIAIRKILKKYDKIHGSVSGRDFKV",
    "response#3": "VPSALYEYFCHTCKDQIKELNECFGVHLRNKDCNNGYISVCFNSNKSSRSSASMQKAKDFFTSAFQKNIKDVQQEKISLTNSYQLNETIMKLNNRFSNLLAKQEENQLLLRGPTKEILNAKNS"
  },
  {
    "reference": "MRYMILGLLALAAVCSAAKKVEFKEPACNVTFKSEANECTTLIKCTTEHEKLIIRHKDKIGKYAVYAIWQPGDTNDYNVTVFQGENRKTFMYKFPFYEMCDITMYMSKQYKLWPPQKCLENTGTFCSTALLITALALVCTLLYLKYKSRRSFIDEKKMP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Include a signal peptide as an essential component of the protein.2. The protein design should include a Di-lysine motif motif to ensure proper function.3. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.4. The protein must exhibit the following characteristics: Binds and retains class I heavy chains in the endoplasmic reticulum during the early period of virus infection, thereby impairing their transport to the cell surface. The designed protein sequence is ",
    "response#1": "MGAASLTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARLLAVERYLXDQQLLGIWGCSGKLICTTNVXWNXSWSNKSXXXXXXXXXXXXXXXXINNYTGLIYTLIEXSQIQQEKNEQXLLALDKWASLWNXFDITKWLWYIKIFIIIVGGLIGLRIVFVVLSIVNRVRQGYSPLSFL",
    "response#2": "LHYWTALDEGAAIGLAWIPYFGPAAEGIYTEGIMENQNGLICGLRQLANETTQALQLFLRATTELRTFSILNRKAIDFLLG",
    "response#3": "MGAASMTLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTNVPWNSSWSNKSLDEIWENMTWMQWDKEVSNYTQMIYNLLEESQNQQEKNEQELLALDKWANLWNWFNISNWLWYIKIFIMIVGGLIGLRIVFAVLSVINRVRQGYSPLSFQL"
  },
  {
    "reference": "MTSRTCLSIILAAGEGTRMKSATPKVLHQIANLPMVAHVARAAEGAGADAVALVVGHGGDAVKKAAEAFAPKADAFVQAERLGTAHAVLAARAAIAKGHDDILVMFGDTPLVEADSLALARQKLAEGAAVVVIGFRPPNPAGYGRLLEKDGRLVAIREDKDCTDEERKIGFCNAGLMAIAGKDALRLLDQVGNSNAKGEFYLTDIVEIADKASLMVVATEASFESVLGINNRAELAEAEAIWQKRRRRQAMLDGATLIAPETVYFSHDTEIGADVLVEPNVWFGPDVKVADGAVIHAFSHLEGADVGPRAEVGPYARLRPGAELHEKAKVGNFCEVKKATVEKGAKINHLTYIGDARVGVGANIGAGTITCNYDGYSKFFTDIGEGAFIGSNSSLVAPIVIGAGAYVASGSVITEEVPGDALAFGRARQKTLPGKGKELRERFASAAAAKKAGAK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein should be able to manipulate the activity of enzymes in the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, Nucleotide-sugar biosynthesis pathway, either by activating or inhibiting their function.2. The designed protein should be involved in regulation of cell shape, cell wall organization, cell morphogenesis by localizing to cytoplasm and exhibiting glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity, magnesium ion binding.3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.4. The protein with the reaction acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate, the Proton acceptor must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVISVVLPNPKGFGRILRKENYIVGIVEEKDADDEQKLINEINTGIYCVSNKWLKENLPLLTNNNAQGEYYLTDIIKLSVEKNICVNTIQPETEIEILGVNSKTQLADLERIYQTNKANELMLMGVTLVDPRRIDVRGKLACGRDVLIDINAVFEGEVELGDGVQIGANAVIRDTSIGAGTVIHPHCVLEQSRIGANCQIGPYARTRPGVEMADDAKLGNFVEIKKSRIGKGSKINHLSYIGDAIIGANVNVGAGTITCNYDGANKHRTTIGDGAFIGSGVELVAPVEIGAGATVGAGSTITKAVPADELVLERSQQKIIPNWNRPKKK",
    "response#2": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLTEHIADPTGYGRVVRDERGQVKRIVEQKDASLAEREIKEVNTGIYCFNAEKLFEALKQVTTDNAQGEYYLTDVLGMLAAEGTPVRAYQTQDPSEALGVNDRVQLAEVERLLRERINSHWMREGVMLRDPNRFDLRGTLQCGMDVEIDANVIIQGNVTIGDNVTIGAGCVLIDCEIDDNTLVRPYSIIESATVAEDCTVGPFTRLRPGAELRNDSHVGNFVEVKNARLGEGSKANHLTYLGDAEIGQRVNVGAGVITCNYDGANKFKTIIGDDVFVGSDSQLIAPVTIADGVTVGAGTTLTKDVEANQLVITRAGVTFIDPGSVFVHAGVTIGRDSVIYPNVYLEGNTKIGRKATIFPNVRIVNADIGAGVVIKDSTVIEESQVRKGASVGPFAHLRPGSQVGDRAKVGNFVELKKAVIGKDSKASHLSYLGDTRIGRGVNIGAGTITCNYDGTHKHTTVLEDGVFVGSDTQLVAPVKVGRGSYIGAGSTITKDVPPGALALSRTVPDKTIPHGVRLADPARFDVRGTLECGQDVEIDVNCVFEGQVAIGAGARIGANCVIANARIEAGAVILPFTHMEGEKKGVRIGAEAIVGPFARLRPGADLGPEVHIGNFVEVKNSTLARGAKANHLAYLGDATVGERVNYGAGAITANYDGANKHRTVIEDDVHVGSNAVLVAPVTIGAGGTVGAGSTISKDAAPGVLSVARGK",
    "response#3": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLSAELDDPTGYGRIVRNAEGAVCAIVEHKDASEAQRAIREVYTGMMAAPTLLLKDWLGRVDNNNAQGEYYLTDIVGMAVADHIPVVSVHTSDPIEVEGVNDRIQLAELERRYQRRTAEALMLKLLDGVTFIDPGSVFVHAGVTIGRDSVIYPNVYLEGNTKIGRKATIFPNVRIVNADIGAGVVIKDSTVIEESQVRKGASVGPFAHLRPGSQVGDRAKVGNFVELKKAVIGKDSKASHLSYLGDTRIGRGVNIGAGTITCNYDGTHKHTTVLEDGVFVGSDTQLVAPVKVGRGSYIGAGSTITKDVPPGALALSRTVQTVIEGWKRPVKQKK"
  },
  {
    "reference": "MASTLPTTWPHESVKFEDVSLRFTEEEWALLDRQQKCLYREIMMENLNNMISVEHHFSKANVMPQLEEVEDCWPMQREIPQDTLPECSGHSLDPEMNSFPAESPLMKIKVVEVLTLNKDMAGPRNALIQSLYPESGEDLNPGNLKPAQQPSKHLTDTEASRQKFRHFQYEESAGPQKSVSQLRKLCHQWLQPSTRSKKQILELLVLEQFLNALPEKLRVWVESQHPEDCKAVVALLENMTSVSKDDAWLACSSEATDELKEKRKDVATLPVTVPPEAPVTFQDVAVDFSQEEWQLLGPMQRTKYHDVMLETLGNLVSVGWEPTLGNRELTPDSPIPVVKPIHDPNPKDLSRNRTQSTLFESISPDEVQGRHTIESNPVGLLQEKGHPQQKLSESSKSQNQTCIDKSQCSLNEVLPRNHVKVKQKGTGKGKGRTNTISMTGGLRIRNQQKDSVEWRDRSGSTPVTHGSSIKNQQLGSEQGKTQTSRDPITLTVPAEFYQEATGSEEGRFRNSSNAMAADAPPKIHQKGPEWHKAGKSNNSMLQGSSAQNHQMGSRAGRARDNSILTHVKIHQKGYKEGKIQGNNNYLKHVKPHRKGSKEERLRELSTCQKHVPYVKQHLKTSGRGKGRKINASIKCGPYIKTYYRGSDVGRLRRANNCRKAISRHAQQISFIKIHKGSQVCQCSECGKMFRNARYFSVHKKIHTGERPYMCMSCGKAFVQSSSLTQHLRIHSGERPFECSECGRTFSDRSAASQHLRTHTGAKPYQCQH",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be involved in regulation of transcription by RNA polymerase II, regulation of histone H3-K9 trimethylation by localizing to nucleus and exhibiting sequence-specific double-stranded DNA binding, sequence-specific DNA binding, DNA-binding transcription factor activity, RNA polymerase II-specific.2. The protein must exhibit the following characteristics: Transcription regulator which may participate in NGFR/p75(NTR)-mediated apoptosis. The designed protein sequence is ",
    "response#1": "TAGTTDSTSNNNTATVISTAPPASSAVTSPSLSPSPSASASTSEASSASETNTTQTTSTPLPSPLGASQVMVTTPGLQTAAAALQGAAQLPANASLAAMAAAAGLSPGLMAPSQFAAGGALLSLSPGTLGSALSPALMSNSTLATIQALASSGSLPITSLDATGNLVFANAGGAPNIVTAPLFLNPQNLSLLTSNPVSLVSAAAASTGNSAPTASLHASSTSTESIQSSLFTVASK",
    "response#2": "MRDEVAEKEKADINVTLVFQGYENTPIMVCVDGIVFSKPDLVTCLEQRKKPWSMKHPGLTQHNC",
    "response#3": "SSPIKAIFPSPASLVATTPSLVTSSTATTLTVNPVLPLTSAAVTNLSLTDQDLRRGCSWEVLRSLF"
  },
  {
    "reference": "MPKIIEAIYENGVFKPLQKVDLKEGERIKLRIEEGILDVIKKYQGKFKLTEKDIEKFLEERR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Possibly the antitoxin component of a type II toxin-antitoxin (TA) system. The designed protein sequence is ",
    "response#1": "GCFSW",
    "response#2": "MPTATR",
    "response#3": "SKLSTI"
  },
  {
    "reference": "MALFPALLLALLAGAHAELPGCKIRITSKALELVKQEGMRFLEQELETITIPDLRGKEGQFHYNITEVQVTELQLNSSELHFQPGQELILQITNASLGLRFRRQLAYWVFSDGGYINASAEGVSIRTGLQLSQDPTGRMKVSNLSCQAFVSRMHMTFGGTFGRLYDFLSTFIISGMRLLLNQQICPVLYHSGMILLNSLLDTVPVRSSVDELVGIDYSLLKDPVVSTDNLDMEFRGAFFPLAEGNWSFLNRAVEPQLQEQERMVYVAFSEFFFNSALESYFWAGAFRLSLEKMPKDLDMLLRATYFGSIVFLTPAEINSPLKLELQVTAPPRCTIKLSGTTVSVTASVSIYLVPPNLPEIQLSSMIMEARLSAKMALRGKALRAQLELSRFRIYSNQSALESLAMIPLQAPLKTVLQIGVMPMINERTWRGVQIPLPEGISFVREVVTNHAGFLTIGADLHFAKGLREVIEKNQPANTKDTQASSAPPPSTAAA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be designed to localize specifically to high-density lipoprotein particle in order to perform its role in vitamin E biosynthetic process, positive regulation of cholesterol efflux more effectively.2. The designed protein should contain one or more Lipid-binding serum glycoprotein N-terminal domains that are essential for its function.3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "FKHLRKYVYNYEAESSSGVQGTADSRSATKINCKVELEVPQICGFIMRTNQCTLKEVYGFNPEGKALMKKTKNSEEFAAAMSRYELKLAIPEGKQIVLYPDKDEPKYILNIKRGIISALLVPPETEEDQQELFLDTVYGNCSTQVTVNSRKGTVPTEMSTERNLQQCDGFQPISTSVSPLALIKGLVHPLSTLISSSQTCQYTLDPKRKHVSEAVCDEQHLFLPFSYKNKYGIMTRVTQKLSLEDTPKINSRFFSEGTNRMGLAFESTKSTSSPKQADAVLKTLQELKKLSISEQNAQRANLFNKLVTELRGLTGEAITSLLPQLIEVSSPITLQALVQCGQPQCYTHILQWLKTEKAHPLLVDIVTYLMALIPNPSTQRLQEIFNTAKEQQSRATLYALSHAVNSYFDVDHSRSPVLQDIAGYLLKQIDNECTGNEDHTFLILRVIGNMGRTMEQVMPALKSSVLSCVRSTKPSLLIQKAALQALRKMELEDEVRTILFDTFVNGVAPVEKRLAAYLLLMKNPSSSDINKIAQLLQWEQSEQVKNFVASHIS",
    "response#2": "LESHHKGHFIYKNISEVLPLPAFSPTLLGDTRMLYFWFSEQVLDSLAKAAFQDGRLQLNLAETELKVVLETWHFNPNQEIIPEVITGFPSPGQVTVHCPRRPTISCQNKGVVVSSPVVMEFLFPHKDGQDSVTHTFEEDIVATIQASYSKKKLFLSLVDFQIKPKTTSNMAESSESIQNFLQLMITTVGIPEIMSRLEVALTTLMNSKGLDLFDIINPEIITRDGFLLLQMDC",
    "response#3": "LGDTRMLYFWFSEQVLDSLAKAAFQDGRLQLNLAETELKVVLETWHFNPNQEIIPEVITGFPSPGQVTVHCPRRPTISCQNKGVVVSSPVVMEFLFPHKDGQDSVTHTFEEDIVATIQASYSKKKLFLSLVDFQIKPKTTSNMAESSESIQNFLQLMITTVGIPEIMSRLEVALTTLMNSKGLDLFDIINPEIITRDGFLLLQA"
  },
  {
    "reference": "MAAIHKIGRRKCAVARVYVSEGTGVITVNKKAFATYFPTATLQYKVLQPMSMTENVTNYDVKVNVYGGGSTGQAEAVRMALARVMCEVGEGNRAILKPEGLLTRDPRMVERKKFGQKKARKRFQFSKR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be involved in translation by localizing to ribonucleoprotein complex, ribosome and exhibiting structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "MPDIERGIRVAVDTGKVILGSNKAIQAIKLGNGELAIMAENTPKNVKADIEAYSKLSEMPVYTFEGSSVELGSICGKPFTVSVLIIQEPGDSNIC",
    "response#2": "ELGSICGKPFTVSVLIIQEPN",
    "response#3": "IPPTTALVMKEAGVEKGSGTPNTQAVGNLPLEAVIRIAKMKMESMLSYDLKTAAKEVMGTCVSVGVTL"
  },
  {
    "reference": "MRVTGIKKNCQDWWKWGMMLLGILMICSAAENLWVTVYYGVPVWKEATTTLFCASDAKGYEKEVHNVWATHACVPTDPNPQEVVLRNVTEKFNMWENNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDELNTNRTTNGQNNQTNTNNITDGIEKGEIKNCSFNITTSLRDKMQKEYALFYKSDVIPIDNSMDNITNTTQYRLISCNASVITQACPKVTFEPIPIHYCAPAGFAILKCNQKNFTGKGECTNVSTVQCTHGIRPVVSTQLLLNGSLAEEDIVIRSDNITDNVKNIIVQLNETVQINCTRPNNNTRRSIPIGPGRAFYATDIIGDIRQAHCNISGAKWNNTLRKISKKLKEYFPDKAIAFNRSSGGDPEIVMHSFNCGGEFFYCDTTQLFNSTWNDTVGNDTEESNNTITLPCRIKQIVNLWQEVGKAMYAPPIRGQIRCSSNITGLLLTRDGGNISTTTTETFRPGGGNMRNNWRSELYKYKVVKIKPLGVAPTSARRRVVQREKRAIGIGAMFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNASWSNKSLGDIWDNMTWMQWEKEIDNYTGLIYTLLEESQNQQEKNEQELLELDKWASLWNWFSITNWLWYIRIFIMIVGGLIGLRIVFAVLSIVNRVRQGYSPLSFQTRLPAQRGPDRPEGIEEEGGERDRDRSGQLATGFLEIIWIDLR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be designed to localize specifically to plasma membrane in order to perform its role in fusion of virus membrane with host endosome membrane more effectively.2. For general function, the protein need meet that The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes The designed protein sequence is ",
    "response#1": "MWVTVYYGVPVWRDADTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVKQMHKDIISLWDQSLQPCVKLTPLCVTLNCNRVNNSSSSNSTNNXXTSSNSTVSPGIKXEMMNCSFNMTXXVXXKKXKVSALFYRLDVXQIDSEQTNSTXYRLINCNTSTVTQACPKVTFEPIPIHYCAPAGFAILKCKDKNFTGTGPCXNVSTVQCTHGIRPVXTTQLLLNGSLAEERVMIRSENLTNNTXTILVQFQNPVRINCTRPSNNTRKSLRIGPGQTFYATGEVIGDIRRAHCNVSTQDWNDTLKEVVTQLRGHFKNKTIIFKNHAGGDLEIVTHSFNCGGEFFYCNTTDLFNSTWDKDGNGTVSSGSNGTITLQCRIKQIINMWQRVGQAMYAPPIQGVIRCDSNITGLLLTRDGGNGNDSQANETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVQREKR",
    "response#2": "MWVTVYYGVPVWKEAKTTLFCASDAKAQDQEVHNVWATHACVPTDPNPQEMILGNVTENFNMWKNDMVDQMHEDVISLWDQSLKPCVRLTPLCVTLNCTNVSIGNATIGNDTQEMKNCSFNATTEIRDKKQKAYALFYKLDIVSLNNNSEYRLINCNTSAITQACPKVSFEPIPIHFCAPAGFAILKCRDEEFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEDKVKIRSENITNNAKVIIVQLVSPVKINCSRPNNNTRTSIRIGPGQSFHATGGIIGDIRQAHCNVSRKEWNSALRQVAAQLGTYFGNKTIIFTKSSGGDLEITTHSFNCGGEFFYCNTSDLFDSAWNSSTWNNNSSQESDSTGANDTITLQCRIKQIINMWQRVGQAMYAPPIQGVIRCESNITGLLLTRDGGNNSTTNETFRPGGGDMRDNWRSELYKYKVVEIEPLGVAPTRAKRRVVEREKRAALGALFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGMWGCSGKLICTTAVPWNRTWSNKTQDDIWNNMTWMQWEREINNYTDTIYSLLEESQNQQEKNEKDLLALDSWNNLWSWFSITNWLWYIRIFIMIVGGLIGLRIIFAVISIVNRVRQGYSPLSFQTLTHHQREPDRPEGIEEGGGEQGRDRSVRLVSGFLALAWDDLRSLCLFS",
    "response#3": "MWVTVYYGVPVWRDADTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVKQMHKDIISLWDQSLQPCVKLTPLCVTLNCNRVNNSSSSNSTNNXXTSSNSTVSPGIKXEMMNCSFNMTXXVXXKKXKVSALFYRLDVXQIDSEQTNSTXYRLINCNTSTVTQACPKVTFEPIPIHYCAPAGFAILKCKDKNFTGTGPCXNVSTVQCTHGIRPVXTTQLLLNGSLAEERVMIRSENLTNNTXTILVQFQNPVRINCTRPSNNTRKSLRIGPGQTFYATGEVIGDIRRAHCNVSTQDWNDTLKEVVTQLRGHFKNKTIIFKNHAGGDLEIVTHSFNCGGEFFYCNTTDLFNSTWDKDGNGTVSSGSNGTITLQCRIKQIINMWQRVGQAMYAPPIQGVIRCDSNITGLLLTRDGGNGNDSQANETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVQREKRAALGALFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGMWGCSGKLICTTAVPWNRTWSNKTQDDIWNNMTWMQWEREINNYTDTIYSLLEESQNQQEKNEKDLLALDSWNNLWSWFSITNWLWYIRIFIMIVGGLIGLRIIFAVISIVNRVRQGYSPLSFQTLTHQREPDRPE"
  },
  {
    "reference": "MRVKVIRKNCQRLWRWGTMLLGMLMICSAAEQLWVTVYYGVPVWKEASTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVMANVTENFNMWKNNMVDQMHDDIISLWDQSLKPCVELTPLCITLNCTDVEGNDTSSTNSTDRKNNNSSTDNSSWEKGEKGEIKNCSFYVNTGIKGKVQEYSLFHKLDVVPIDKASSNGSGRFILIHCNTSVIKQACPKVSFEPIPIHYCAPAGFAILKCNNKKFNGTGPCTNVSTVHCTHGIKPVVSTQLLLNGSLAEEDIVIRSENLTDNAKTIIVHLKEPVVINCTRPNNNTRKSIHLGAGRALYTNGEIIGNIRHAHCNISKTQWHQTLEKIVTKLREKFENKTIVFNQSSGGDIEIVMHSFNCQGEFFYCNTTKLFNSTWNTNSTWNNTEESNITLPCRIKQIINMWQRVGKAMYAPPIRGQIRCTSNITGLLLTRDGGNNSTEDSETFRPGGGNMKDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGVIGAMFLGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQNNLLRAIEAQQRLLQLTVWGIKQLQARLLAVERYLRDQQLLGIWGCSGKLICTTTVPWNTSWSNKSLDYIWDNMTWMQWDREVSNYTQIIYTLLEDSQNQQEKNEHELLELDKWANLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVISIVNRVRQGYSPLSFQTHLPARRGPDRPEGIEEEGGERDREESIRLVHGFLPLVWD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Incorporating a Di-leucine internalization motif, YXXL motif; contains endocytosis signal motif is critical for the successful design of the protein.2. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.3. The protein must exhibit the following characteristics: Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain.4. Prioritize Polar residues, Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.5. The designed protein should contain one or more Human immunodeficiency virus 1 envelope glycoprotein Gp120, Retroviral envelope protein GP41-like domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MKVKEMRRNYQHLWRWGTMLLGILMICSAAEKLWVTVYYGVPVWKEATTTLFCASDAKAFKAEAHNIWATHACVPTDPNPQEIKLENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDATNNATDNDTNTSSTTEGGEIKNCSFNITTKIRTKVKDYALFYKLDVVPIDNDNTSYRLINCNTSVITQACPKVSFEPIPIHYCTPAGFAILKCNNKTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVIRSSNFTDNAKVIIVQLNKSVEINCTRPNNNTRKGINIGPGRAWYTTGDIIGDIRQAHCNLSRTEWNNTLKQIAEKLREQFGNKTIIFNQSSGGDPEIVMRSFNCGGEFFYCNTSKLFNSTWYNNGTWNNASTGNDTITLPCRIKQIVNMWQEVGKAMYAPPIAGQIRCSSKITGLLLTRDGGNENKTKDNNTETETFRPGGGDMRDYWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLALERYLTDQQLLGLWGCSGKVICTTNVSWNSSWSNKNQTEIWDHMTWMQWDREISNYTDTIYRLLEESQIQQENNEKDLLALDNWNSLWNWFDITKWLWYIKIFIMIVGGLIGLRIILGVLSIVKRVRQGYSPLSFQTLTHHQREPDRPERIEEGGGE",
    "response#2": "MRVMGTLRNCQQWWIWGILGFWMLMICNGKGLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNIWATHACVPTDPNPQEVVLGNVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNLRNITNTSSNTSSTNNTSSNVSNSNELLKCSFNMTTVIRDRRQKMDALFYRLDVVPITDGQNSTQNSSYRLINCNTSAITQACPKVTFEPIPIHYCAPAGFAILKCNDKEFNGTGPCKNVSTVQCTHGIRPVVSTQLLPEMAVLAEGEVMIRSENLTNNAKVIIVQLNESVAINCTRPNNNTRKGIHIGPGQAFYATDNIIGNIRQAYCNVSRTGWNKTLQNVVTQLQKHFKNATIVFDKHSGGDLEITSHTFNCRGEFFYCNTSKLFNSSWNGTEDPIILPCEIKQIINMWQRVGQAIYAPPIPGVIRCNSNITGILLTRDGGTNSTNETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTEAKRRVVERGKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQXNLLRAIEAQQHMLRLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTSVPWNSSWSNKTQKEIWENMTWLQWDKEISNYTDIIYSLIEESQNQQEKNEQDLLALDKWSTLWSWFDISNWLWYIXIFIMIVGGLIGLRIVFAVLTIINRVRQGYSPLSFQTLTHHQREPDRPERIEE",
    "response#3": "MKNCSFNVTTEVKDKKKQVHALFYKLDVVQMDDNRTNTNYSSYRLINCNTSAITQACPKVTFEPIPIHYCAPAGFAILKCNDKKFNGTGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKTIIVQLNESVTINCTRPNNNTRQSTHIGPGKALFTTNIIGDIRQAYCNISKAEWDKTLQQVGKKLEVLFDNKTAIFRPSSGGDPEITTHSFNCGGEFFYCNTSSLFNTSNNTGENNSTITGENNSTITLKCRIKQIINMWQGVGTAMYAPPIAGPISCSSNITGLLLTRDGGINSNKSRNETFRPGGGDMRDNWRSELYKYKVVRLEPLGLAPTNAKRRVVQREKRXLGXMXAXTLWVTVYYGVPAWEDADTILFCASDAKAHSTEKHNVWATHACVPTDPAPQEIFLENVTENFNMWKNHMVEQMHEDIKSLWDESXKPCVKLSPLCVTLHCTNATINGTSEGTNSTVKVNNKEEMTNCSFNVTTEIRDKKKAEYALFYKLDVMPIDGKNNSNNSEYTLINCNATTIKQACPKVSFEPIPIHYCAPAGFAILKCRDKNFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEESIIIRSENITDNAKTIIVQLNQSVAITCTRPYNNTRKSIHIGPGQAIYATDAIIGDIRQAHCNISGKNWTEMLQNVTAKLEEILQKNITFNSSAGGDLEITTHSFNCGGEFFYCDTSQLFNGSSSWNGTITLPCKIRQIVRMWQKVAQAMYAPPIIGNITCKSNITGLLLTRDGIRNGTEIFRPGGGNMKDNWRSELYKYKVVRIKPLGIAPSKARRRIVGREK"
  },
  {
    "reference": "MHRYRSHTCGALRESDIDSNVRLSGWCHRIRDHGGLLFIDLRDHYGITQCVVDPDSKAFRLAETLRSEWVVKFDGRVRRRPDGTDNPELPTGAVEVFINEIEVLGEAAELPMPVFGDQEYPEEIRLKYRFLDLRRDRLHQNIMTRGKIIDSMRARMKEVGFFEFQTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLEMSFVTQEDVFAAMEPVITGVFEDFADGKPVTKNWPRIPFAEALRKYGTDKPDLRNPLIMQDVSEHFRGSGFKVFARMLEQEGNEVWAIPGPAGGSRAFCDRMNSWAQGEGQPGLGYIMWREGGEGAGPLANNIGPERTAAIRDQLGLKEGDAAFFVAGDPQKFWKFAGLARTKLGEELNLIDKDRFELAWIVDFPMYEYNEDEKKVDFSHNPFSMPQGGLEALQTQDPLTIKAFQYDIACNGYEIASGGIRNHKPAAMVKAFEIAGYGEETVIERFGGMYRAFQYGAPPHGGMAAGVDRIVMLLCGTNNLREISLFPMNQRAEDLLMGAPSDVTTQQLRELHIRLNLPQN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have a fast turnover rate for ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-   aspartyl-tRNA(Asx).2. A protein that can perform nucleic acid binding, ATP binding, aspartate-tRNA ligase activity, aspartate-tRNA(Asn) ligase activity in cytoplasm to support aspartyl-tRNA aminoacylation.3. The designed protein should have a binding site that is specific to the target L-aspartate, ATP ligand.4. For general function, the protein need meet that Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).; Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn) The designed protein sequence is ",
    "response#1": "MRMRSEVNRIARDVLHGRDFVEVETPTMTRSTPEGARDFLIPARLQPGNWYALPQSPQLFKQLLMVGGLERYFQIARCYRDEDFRADRQPEFTQLDIEMSFVDQDDVIELGEEIISALWRETAGHEIPRPIQRMTYADAMARFGTDKPDLRFGLELTEMTEYFADTPFRVFRAPYVGAVVMPGGADQPRRQLDAWQDWAKQRGAKGLAYVLVGQDGTLSGPVAKNLSDGERDGLVKAVGAAPGDCVFFAAGDRSSSRALLGAARLEIGERCGLIDHDAWSFVWVVDAPMFESIDDTDDVAVGSGRWTAVHHPFTSPNADWVDNFESDPANALAWAYDIVCNGNEIGGGSIRIHRSDVQKRVFELLGISEEQAQDKFGFLLEAFKYGPPPHGGIAFGWDRICMLLAGADSLRDVIAFPMNRRPR",
    "response#2": "MRMRSEVNRIARDVLHGRDFVEVETPTMTRSTPEGARDFLIPARLQPGNWYALPQSPQLFKQLLMVGGLERYFQIARCYRDEDFRADRQPEFTQLDIEMSFVDQDDVIELGEEIISALWRETAGHEIPRPIQRMTYADAMARFGTDKPDLRFGLELTEMTEYFADTPFRVFRAPYVGAVVMPGGADQPRRQLDAWQDWAKQRGAKGLAYVLVGQDGTLSGPVAKNLSDGERDGLVKAVGAAPGDCVFFAAGDRSSSRALLGAARLEIGERCGLIDHDAWSFVWVVDAPMFESIDDTDDVAVGSGRWTAVHHPFTSPNADWVDNFESDPANALAWAYDIVCNGNEIGGGSIRIHRSDVQKRVFELLGISEEQAQDKFGFLLEAFKYGPPPHGGIAFGWDRICMLLAGADSLRDVIAFPKAPKDAQLFSGPANDAKGRVAVIRVPGGASMTRKQIDEYTKFVGIYGAKGLAWMKVNDIDAGLEGLQSPILKFLGEDVAKQVLSRVEAQSGDILLFGADSATVVTEAR",
    "response#3": "MRTHYCGEVTDMAIGQTVRLKGWVQKRRDLGGLIFIDLRDRTGIVQIVFSPDVSKEALQVAETIRSEYVLDVEGTVVQREEGQVNPNLPTGTIEVHAMHVTILSEAKTPPFLISDKTDVSEDVRLKYRYLDLRRPVAEDNTPVGPDEALRLRSAANKAARTVLDSHDFTEIETPTLTRSTPEGARDFLVPARLRPGTFYALPQSPQLFKQLLQVAGMERYYQIARCYRDEDFRADRQPEFTQLDVEMSFVDQDDVIALGEEIISEVWKLIGYEIKTPIPRMTYADAMRRYGSDKPDLRFDIEITECTEFFQDTTFRVFKNEYVGAVVMTGGASQPRRQLDAWQEWAKQRGAKGLAYILVGEDGELSGPVAKNITDAERAGIAAHVGAQPGDCIFFAAGDTKSSRALLGAARGEIAKKLDLIKEGDWAFTWIVDAPMFEPAADATASGDVALGNSKWTAVHHAFTSPKPEFLDNFDTNPGDALAYAYDIVCNGNEIGGGSIRIHQRDVQERVFEVMGITGEEAREKFGFLLDAFAFGAPPHGGIAFGWDRIVSLLGGFDSIRDVIAFPKSKGTCDLMAGAPAPVTPEQLKELSVRVAQ"
  },
  {
    "reference": "MEGTCSEEENLAKRVTPTAVTSGTYGTHVVDTTDLALSDVVIGCGSTIYETSDSIGSDVMLGLSSHGVSSVSGLDRDLNSFKKRIDANTEEQREHADMMVGLQRKIEEYRRRIVDAEKQVSIQKANDDVSFSIKETADTWLPDMKSDAADYEWASRLDEERRRNDELHMQITQQQVDLQRLQKYFEANMQEKEKIYQTREKNLAYYLNAEQRKMLDLWEELQRVRRQFADYKEQTERDLENQKNEVAKVTQSVGGMAGRLNTSSHGDSGLVQDVVLLEAMRRFRELQAVPVGASADDYNALMKKYEETVERVIELESHGDGSTAKMLSLEAELRRTKDKFIECSEFLRKLGDLAAGSYRGDERTSKIVSLSPGGMSLPSEIYRSVRNILRNHDSEMQHIQRKLKNSDTQVCELTTRLEGTEEARRRSDKQLVDAKREINIQQRAVDDANRELRRVEDRLHIMESEKIVAENARQQLEEEVRRLTLQVDQSKADGERRVVEEGEIQKRIVEDEYRSMISELTRRMNAFQDENKRLKNDLGCTKERLKNVEFEYNSTVRKLEDKDIALKHLEDTKLDLLKDLENQRTRYDAVTNELDTLQTTFETSTKNIAQLEANIKEINLMRDEISKEKDSLAQKLADVTHKLEIETVRREDIQRSCVGHSEDVEKQRLQIIEYEREVMALRRLNDELDTNVKTGQAKVTSLENSIISVQTEVTKLTTLNDKLQKEKQSIMSSKQKADTDVDLLKEKLRKLEQECDKLKEENKALHED",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design must be focused on enhancing the efficiency of meiotic cell cycle. The designed protein sequence is ",
    "response#1": "NMRRRATGYPAIVVPFLNGSLPQADLPPLRTIEDIDNLTREQCLTFIQGYGIPIDSSDTVYLKEKLRDAIGML",
    "response#2": "NMRRRATGYPAIVVPFLNGSLPQADLPPLRTIEDIDNLTREQCLTFIQGYGIPIDSSDTVYLKEKLRDAIGML",
    "response#3": "NMRRRATGYPAIVVPFLNGSLPQADLPPLRTIEDIDNLTREQCLTFIQGYGIPIDSSDTVYLKEKLRDAIGMI"
  },
  {
    "reference": "MAKVKVGINGFGRIGRLVARVILQRDDCELVAVNDPFISTDYMTYMFKYDSVHGQCKSHEIKLKDEKTLLFGETPVAVFGCRNPEEIPWGQAGADFVVESTGVFTDKDKAAAHLKGGAKKVVISAPSKDAPMFVVGVNEHEYKSDLNIVSNASCTTNCLAPLAKVINDRFGIVEGLMTTVHAMTATQKTVDGPSMKDWRGGRAASFNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTCDVSVVDLTVRIEKAASYEQIKAAIKEESEGKLKGILGYTEDDLVSTDFIGDNRSSIFDAKAGISLNDNFVKLVSWYDNEWGYSTRVVDLIMHISKCQ",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be optimized for NAD binding, glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity activity in order to improve glucose metabolic process, glycolytic process.2. The D-glyceraldehyde 3-phosphate, NAD(+) binding site should be located in a region of the protein that is accessible to the ligand.3. A protein that is capable of catalyzing reactions within the glycolysis, pyruvate from D- glyceraldehyde 3-phosphate: step 1/5 pathway with high efficiency and specificity.4. The Nucleophile active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.5. For general function, the protein need meet that Key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3- phospho-D-glyceroyl phosphate; Essential for the maintenance of cellular ATP levels and carbohydrate metabolism . The designed protein sequence is ",
    "response#1": "MKVVINGFGRIGRLVLRQILKRNSSVEVLAINDLVPGDALTYLFKFDSTHGRFPEDVRCEADHLIVGKRKIQFLSERNVQNLPWKDLGVDLVIECTGLFTKKEDAEKHIQAGAKRVLISAPGKGDIPTFVMGVNHKTFNPEKDFVISNASCTTNCLAPLAKVINDNFGLTEGLMTTIHSYTATQKTVDGPSSKDWRGGRTAAQNIIPSSTGAAKAVGKVIPSLNGKLTGMSMRVPTANVSVVDLTCKTEKPVTYDQIKQTIKKASEGELKGILGYSEDALVSSDLNGDSRSSIFDASAGIALNDHFIKLISWYDNEYGYSNRVVDLIAHMAK",
    "response#2": "MKVVINGFGRIGRLVLRQILKRNSSVEVLAINDLVPGDALTYLFKFDSTHGRFPEDVRCEADHLIVGKRKIQFLSERNVQNLPWKDLGVDLVIECTGLFTKKEDAEKHIQAGAKRVLISAPGKGDIPTFVMGVNHKTFNPEKDFVISNASCTTNCLAPVAKVLHENFEIVEGLMTTVHAVTATQKTVDGPSAKDWRGGRGAAQNIIPSSTGAAKAVGKVIPELDGKLTGMAFRVPTPNVSVVDLTVRLGKECSYDDIKAAMKAASEGPLQGVLGYTEDDVVSCDFTGDNRSSIFDAKAGIQLSKTFVKVVSWYDNETGYSHKVVDLIAHMAKTDAN",
    "response#3": "MKVVINGFGRIGRLVLRQILKRNSSVEVLAINDLVPGDALTYLFKFDSTHGRFPEDVRCEADHLIVGKRKIQFLSERNVQNLPWKDLGVDLVIECTGLFTKQVGINGFGRIGRIVLRASLSNPEVQVVAINDPFIPLEYMVYMFKYDSVHGRFQGTVEAKDGKLVVNGKEISVFSERDPAQIPWGSVEAAYVVESTGVFTDKTKAHIDKGASCTTNCLAPLSKVIDDNFGIIEGLMTTVHASTATQKIVDGASDKDWRGGRGALQNIIPSSTGAAIAVGKVLPNLNGKLTGMAFRVPVSNVSVVDLTVRYKKSATYSEICQKIQQASQKEMKGIMGYTEDEVVSTDFNGEELTSIFDAKAGLSLNKNFVKLIAWLCRVIYPIDRELGVKKVRVTLARRATDPNDIKKGPINAIVPDPIKLPSHHGPDIKSVLPHINITTAALKIPTTLMHLHTVNMEVNTDCTAEDINRIFSSQSRIRFMSQGINSTAEIIELARDMGRPRNDMWENCIWPESITVHEREFYFFQAPNYGLCRVIYPIDRELGVKKVRVTLARRATDPNDIKKGPINAIVPDPIKLPSHHGPDIKSVLPHINITTAALKIPTTLMHLHTVNMEVNTDCTAEDINRIFSSQSRIRFMSQGINSTAEIIELARDMGRPRNDMWENCIWPESITVHEREFYFFQEPIPYTRSSFVLTRTLSALQEYIDYVYAVMIRRAADPNDSKRGPVNAITPSVTVPSHHGPDVQTVIPINIETMAFVVPTTLMHVHSVMIELKKPITREDVIDIFENTTRVLLFEKERGFESTAQLIEFARDLHREWNNLYEIAVWKESISVKGNRLFYIQA"
  },
  {
    "reference": "MSELLTAAQMRAIEQAAIESGDVTGLELMERAGQGVVEAIFEEWPEFDCVSQDAGGQPPDLRHISGQMKRAVVLCGPGNNGGDGFVVARLLHERGWQVEVFFYGDAEKLPPDARVNYERWSALREVMSWGDLHIRDAYEDDEECQILVIDALFGTGLTRMMPTDTSEMWSGFMPSAMAHYSQVRGADKFVAIDFPSGVCSDSGKDFGALPTHLTVTFHSAKPGHYLVSDGGNYGGVTACNKVVVKDIGLGHADLGDRTSREALVALTGEKRIGRVVASLVESPSAYLEKWSEEHKFSHGHAVILSGGPGKTGAARLAARGALRIGAGLVTLGVPPAAQQEVACQITALMLQRVGDAQAFEKALEDQRLNALCLGPGMGFERARELVPVALRSRRATVLDADALSAFSEDPETLFERLHEQVVLTPHGGEFARLFPDIAAQLHAPPTKGPAYSKVDATRAAAARAGCTVLFKGADTVIASAEGTCAVHAAVYDRAAPWLATAGSGDVLAGFITGLLARGFAPKMAAESAAWLHVECARAFGPGLIAEDLPEILPKILHKLDG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein with ADP-dependent NAD(P)H-hydrate dehydratase activity functions is required to enable nicotinamide nucleotide metabolic process.2. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration The designed protein sequence is ",
    "response#1": "LIAAGTKRMSGAGLLCAKAALRAGCGLATWAVPASLATHLLGRVPEAMLAGLADAGHGDWTHVNAEAILALAEDCDALALGPGMGRYEGDSQWLRGLWEGAGCPLVLDADALNMLAEAPDFADWQPRRAATVLTPHPGEMARLAGVSTREVQRDRIGLARRYAEQHGVTLVLKGARTVVATPDGAVYVNVNGNAGMATAGAGDVLAGIIASLLSQGFDAAQAACLGVYMHGAAGDRAALKRTSPASLLAGDLS",
    "response#2": "HAYAQKLLPPPRPANSHKGMFGSIGVIGGSAGMVGAALLAGTAALKLGAGRVYLGLIAGDALSVDMAHPELMLRPIHELFKLGHLNCLVVGPGLGSGPDACFWLSCALESDLPLVLDADALNLIAVHSRVADLLRKRLHERKAPSILTPHPAEAARLLNTDISTIQGDRMGAAADLVENFNCCVVLKGAGSICAVPAGKRYISISGNPGLSSAGTGDILSGIAGAFLAQGLSPENALLLSVYLHGAAADVLQAHHGGPIGMIASEIPDAARDLLNRLLVVAGSEGMTGAAALAGEAAMRVGSGLVSVAVPQSLRTVIEMKLTECLKLGLEESEPGVLGEGAVRQLTPHLHRYHATLLGPGLSTRGEVARVVVALIERAEHPMVLDADALNILAGTLESLEVAKAPLILTPHPGEMARLLRTSTARVQENRLSIAHDFATRHGVVLVLKGARTLIATPEGEVFVNPTGNAGMASGGTGDVLAGMIGGFLVQGHPPRTAALLGVYLHGLAGDLAAEALGERSFIASDLLDRWPPPRPVDAHKGTFGHALLLAGSPGYHGAAVLAARAAQRARPGLISLFTDAAVYGPVASQLQAVMVHAVGDNRFNLPEKGTALLVGPGLAAPNAKNRFAEQLLSAWRTTHHPVVVDASALDWLAPRHSPPRENIRVLTPHPGEAARLLRSSAGQVQADRLQALRTISAQRGHCWVILKGRHTLIGRSEGPVYVNGSGNPDLAQGGAGDILAGLLVGYLAQPELQADPARTLALAVWQHGEAADRLTAAGRRWVPEE",
    "response#3": "PDSHKGTFGHLLVVAGSEGMTGAAALAGEAAMRVGSGLVSVAVPQSLRTVIEMKLTECLKLGLEESEPGVLGEGAVRQLTPHLHRYHATLLGPGLSTRGEVARVVVALIERAEHPMVLDADALNILAGTLESLEVAKAPLILTPHPGEMARLLRTSTARVQENRLSIAHDFATRHGVVLVLKGARTLIATPEGEVFVNPTGNAGMASGGTGDVLAGMIGGFLVQGHPPRTAALLGVYLHGLAGDLAAEALGERSFIASDLLDRLV"
  },
  {
    "reference": "MTDRTPPQGRARRLFSRTSWPEARFLADVLRTETFGGGLLLLGAVLALLWANSPWADSYAALASWVPWPGGSDLHLDLDLATWAADGLLAIFFFVVGLELKREFVAGDLRDPRRAALPVVAAIGGMIVPALIYVGINLAAGGENLRGWAIPTATDIAFALAVLAVIGSHLPQGLRAFLLTLAVVDDLLAITVIAIFYTGDFKLTPLLVALLPIALFGLLVQRRKTWWWALIPLAVVAWTLVHESGVHATVAGVLLGFTVPVLRGREGDRHGLAEHLEHRWRPVSAGFAVPVFAFFAAGVSLRGADLGGIVTDPIVVGIVAGLVLGKVLGIFGSTFMLARFTRAELDRDITWTDLLGVSLLAGIGFTVSLLIGELAFEGGTAGDNVKAAVLTGSVIAALLASAVLSRRNKAYRRIAAKERLDSNRDGVPDVFQHRDG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be optimized for sodium ion transmembrane transporter activity activity in order to improve regulation of pH.2. For general function, the protein need meet that Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons.3. The protein should be designed to catalyze the following reaction 2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out). The designed protein sequence is ",
    "response#1": "MW",
    "response#2": "GFAK",
    "response#3": "GRRSA"
  },
  {
    "reference": "MASSKFEEVRDKVKEFPTQSGVYLMKNTADKIIYIGKAKNLRNRVRSYFNDSKDHSPKTRLLVSNICEVEYILTKTEVEAFLLEASLIKKHRPKYNIRLRDDKAYPYIRFSWGDQAPRLYLARKVKKDGSLYFGPYTSGLAVHGTIKFLNRTFKIRDCTDAVFKSRTRPCMTYQIGRCTAPCVKYITMEDYRNEVEGAKLFLKGQNKKVIKSMTEKMMGAADEEKFEVAARLRDSIEAIKAILQKQAVINDTSEKDQDALGYYGDERGCLIETVHIRAGRVIGTRPHYLPHFDPNDAVEDPREWLVDFLNQYYEDNFIPDEVLLPLDIGNDLTKLMEEVLKERSGSKTTVRFATDERGRNLVDMAHENAKSHFLKYVSKSEEKLRGLEEIKERFNLPERPRRIECYDISTFQGAETVASQVVFEDGVPAKEHYRRYKIKTVQGINDFASMYEVLSRRFKHTEYDDPQLIVIDGGKGQLSQAMKILEEIGRKDIPVVGLAKARTESDFQKQEVESTEERFFLPGRSNPVIFKHNAEALYILSGIRDEAHRFAITYHRKLREGTSLESELDYVVGLGEKRKKVLLTQFNSIDEIKMADPEEIAKLKGFNRVLAERILLQLNESEEEETEVEE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must possess excinuclease ABC activity to support SOS response, nucleotide-excision repair under certain conditions. The designed protein sequence is ",
    "response#1": "VLALARDGNRGRVIVLEIRDGKLLGRHSLDLQGIAERSDAEVLAAFLKQYYREAGLIPPEVLLPVPLGEEQGLIAEWLGLRRQGRVRLHVPRRGRKRELVRLAEQNAAEARAQMEFERDAGEALAELAAVLGLDQAPARLEGYDISNLQGAQTVGVMVVFEDGRPAPGEYRQFRVRETAGTPNDFAAMREVVARRFARAREEGKLIATGRLSSRDARFHRLPDLVLVDGGKGQLSAALP",
    "response#2": "VLALARDGNRGRVIVLEIRDGKLLGRHSLDLQGIAERSDAEVLAAFLKQYYREAGLIPPEVLLPVPLGEEQGLIAEWLGLRRQGRVRLHVPRRGRKRELVRLAEQNAAEARAQMEFERDAGEALAELAAVLGLDQAPARLEGYDISNLQGAQTVGVMVVFEDGRPAPGEYRQFRVRETAGTPNDFAAMREVVARRFARAREEGKLIATGRLSSRDARFHRLPDLVLVDGGKGQLSAALG",
    "response#3": "VLALARDGNRGRVIVLEIRDGKLLGRHSLDLQGIAERSDAEVLAAFLKQYYREAGLIPPEVLLPVPLGEEQGLIAEWLGLRRQGRVRLHVPRRGRKRELVRLAEQNAAEARAQMEFERDAGEALAELAAVLGLDQAPARLEGYDISNLQGAQTVGVMVVFEDGRPAPGEYRQFRVRETAGTPNDFAAMREVVARRFARAREEGKLIATGRLSSRDARFHRLPDLVLVDGGKGQLSAALK"
  },
  {
    "reference": "MKELVVDLKEKSYSIIIKKGLINELSNEINKVYKGKKIFILTDENVNYHYGDKVKDSLINNGYDVKKIVLKPGEETKSFNTLPKIYNEFLDFKLTRSDLIITLGGGVIGDLGGFAASTFLRGIDFIQVPTSLLAQVDSSVGGKVAVDLDRGKNLVGSFYHPKVVLIDPDVLITLEEKFFKDGMAEVIKYGCIKDKEFFYKLKEFKSKDEVLDNIEDIIYTCCNIKRIVVENDEKDKGERMLLNFGHTLGHAIEAYYNFNKYTHGEAVGIGMYKIIKISEEKGIMPKGCADEIKDILIQYSLPYDIEIENSDEILETISLDKKNINSVLKIVLLESIGQSFLKSTNIEFFK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have metal ion binding, 3-dehydroquinate synthase activity within cytoplasm to regulate aromatic amino acid family biosynthetic process, chorismate biosynthetic process, amino acid biosynthetic process.2. We need a protein that can perform 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-   dehydroquinate + phosphate with high efficiency.3. The protein must exhibit the following characteristics: Catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ)..4. Ideal protein should have a binding affinity for key enzymes in the chorismate biosynthesis, Metabolic intermediate biosynthesis, chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7 pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MTTTHLRGEVTIPPSKSILHRALICASLAKGSSTIHHVVFSEDIRATMECMKQFGASFEVHDDAITVHGVESVPQKASFSCQESGSTLRFLIPIASAFGIDAFFTGKNRLVTRPLDLYESALSSHGMTVQYAGQLPFAVKGQLCSGVYQIKGNVSSQFITGLLFALPLLPEESIIEVLPPFESKSYVKITLQVLKQFGIEVFAEGDRYRIPGGQQYKPCDFSVESDFSQAAFFLVADALGASVTLRQFDLRSIQGDRAVLDVLQKAGCRIICREGALTCKPAQLTAFEIDGSDIPDLVPILCVLACFCKGTSRIKQVKRLRIKESDRIASTMELIKKMGGYAHYDEENDCLTVEGEKQLHGAEIQSYNDHRIAMCAAVAAAVAEGVTSIKNAACVKKSYPAFFHNSSFS",
    "response#2": "GIPEAIKHGCIADAYIWEFLDNCRDRLFSSQEILHEFIKRNCMVKAAIVAKDPKDQNLRKTLNFGHTIAHAIETLSKGHISHGLAVSVGMMIEMRISLESGIMKNPYLIEQLDNLLKYFCLPTALQELGSLIPQVRSEGIRATGIRGLAEVFKYGILCDRELFLTVKSLFEDCEPLNNVSWERYSYLIHKSCEIKADIVSQDEIDTGIRMLLNLGHTFAHALEGATSYNYFKHGEAVMWGLAMSAELSYKLNKLTYKDYQAIAELPELTKVPEVPIQVKDPNIIEELLLRDKKGNELKIVVIDGRVPTPLAEYANVDVRNVILVGHTSEVLRSVFNRSRIGSPIR",
    "response#3": "LVMEAGDMNKGFVSLAQIWTALSNEGASRNSLLVNLGGGMITDMGGFAGATFKRGIRTINIPTTLMASVDAAVGGKTGINFNGLKNEVGSFYPPLCVFIDCDFLRTLDRDNDTKVLFDAVTKYFQVTPVEGKTLVLLGAGGSSRAIAFEAIRRGARVMILNRTPEKAIKLANHLGCCGGSLGDLEKISYDILINTTPHSCPIDFQVNQARTGLAEVFKYGILCDRELFLTVKSLFEDCEPLNNVSWERYSYLIHKSCEIKADIVSQDEIDTGIRMLLNLGHTFAHALEGATSYNYFKHGEAVMWGLAMSAELSYKLNKLTYKDYQAIAELPELTKVPEVPIQVKDPNIIEELLLRDKKGISYTIQRLVSGIPEAIKHGCIADAYIWEFLDNCRDRLFSSQEILHEFIKRNCMVKAAIVAKDPKDQNLRKTLNFGHTIAHAIETLSKGHISHGLAVSVGMMIEMRISLESGIMKNPYLIEQLDNLLKYFCLPTALQELGSLIPQYSQYLEEKQN"
  },
  {
    "reference": "MTHFPVNIASDKQEFDPERWAKTPTTESSVNGENGTAPTSGLPSRHPSTGISVLIVGAGMGGLMTALECWRKGHDVAGILERSEGPVYSGDIIVMQPSAVSIIRHWPDMLHDMKAEQVHAVVSYETHDGRHIYGPTVPSFNDPEHLETRKGPFVAPAQVRRKFYRMLLRQVARCGLRVEYGKTVKSYFEDEKDGKGGVIIATTGEAEVRVADIVVAADGLKSPSEILIAGQHVPPRSSGLSIYRTAFPKDLAMQNELVRKRWSDSPPIWEYWLGPGMYLGVFVGDDIISFGFTPRDDIVEGTATESWEPDTDPETVAQAMLSGAGDWDPAVLALIRSAPKGAIVHWPLLWRDLRREWTSPAGRVVQVGDSAHSFIPTSGNGGSQALEDAITLATCLQLAGSSQRAYLGTKIYNLLRYERVSCAQKMSFVNSQLKTGTDWDAIWKDPAKIRTRFPKWIFQHDPEAYAYEKFGEAFAHLLDGREFVNTNYPPGHEFRAWTVEEVWRNIADGKRVEDLLDGDWS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that The pathway begins with the synthesis of atrochrysone thioester by the polyketide synthase (PKS) mdpG2. The designed protein should be optimized for monooxygenase activity activity in order to improve monodictyphenone biosynthetic process, secondary metabolite biosynthetic process.3. Ideal protein should have a binding affinity for key enzymes in the Secondary metabolite biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "ASPDTLHLVTDAPLPTLGQDDSVLIRVVCVAINPVDGKSAEMSPTPGATSGTDFAGIVVALHGDAKSRTETADTIKTGDRVMGFVFGNNPHVLGNGAFAEYVTLPRRFLWRVPDHMSLEAAASLPVGVASVGMALHYLRISMSSLLKAVSRSIAAPSASQPHDGAFDSDANVFILVYGGGTSTGAIAIQILKAAGFHPITCCSSESASRAKRLGAVATFDYQSATCGRDIRDYTNDSLTLAIDCLSESASMAICYEAMGSAGGRYVSLDPFPVRGCVRRSIVPDWICSFTQFGQSIPWAPPYNLDERPDDHRLAEEWYHLAQKLLDAELIEAPTLEIRSGGLLHVPEGVAAVKLGQL",
    "response#2": "VRASSLRNGVRAPNPRVCLATDYTAYLYDKMMGVGRFHLLLFGSDLQGPVRQRLAVLAGELRKREGFYERFGGREMFNLILVVKTLSHQTAELLEGDLAPLKDHATTVYDDRAPDDDAHYWYGVNHARGALVVVRPDLAVGVSVWPEEIGKLNKYFASFLLN",
    "response#3": "VRASSLRNGVRAPNPRVCLATDYTAYLYDKMMGVGRFHLLLFGSDLQGPVRQRLAVLAGELRKREGFYERFGGREMFNLILVVKTLSHQTAELLEGDLAPLKDHATTVYDDRAPDDDAHYWYGVNHARGALVVVRPDLAVGVSVWPEEIGKLNKYFASFLLD"
  },
  {
    "reference": "MKQFCWARLVQLCLLAAWTCVWLVEGCGPGPGYGIRSRPRKLTAMHYKQFFPNFSENNLGASGRAEGKITRDSERFNELVCNYNPDIVFKDEENTDADRLMTKRCKDCLNRLAIAVMNQWPGVHLRVTEAWDEDGHHPQGSLHYEGRAVDITTDDRETEKYGLLAQLAVEAGFDWVHYESKYHVHCSVKADHSVAVERGGCFPGWARVTLAGGRQKTLSSLAPGDRVMALSDTGQVVYTRVLSFLHQDQESVSTFLSLETEEGYTLVLTPHHLVFLAPNCGHDISEYQARFASRAKTGDCVLISTADSQTLPSPIVSVSVAESVGVYAPLTEAGTLFVDRVLASSYALVEDHRLAHWAFGPLRLFYSLKELLWAEAADQTTTGSISLTNIRSHFSTLTIKKEGINEGFLHKHNNLTEPFRMKIQEKRLSKIHWYARLLYRFAWVLLDSNLFYP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein have Hint domains with specific sequence motifs that are crucial for its activity.2. The desired protein should be able to perform cholesterol + glycyl-L-cysteinyl-[protein] + H(+) = [protein]-   C-terminal glycyl cholesterol ester + N-terminal L-cysteinyl-   [protein]3. The designed protein should be involved in protein autoprocessing, cell fate specification, smoothened signaling pathway by localizing to extracellular space, plasma membrane and exhibiting calcium ion binding, transferase activity.4. The designed protein should be able to compete with endogenous proteins or inhibitors for Ca(2+) binding. The designed protein sequence is ",
    "response#1": "CGPGPNYGVRPKGRRVTPMAYKQFVPNFSENNLGASGRSEGKITRNSERFNELVCNYNPDVIFKDEENTKADRFMTKRCKDCLNVLAIAVMNQWPGVRLRVTEAWDEDGHHPPNSLHYEGRAVDITTSDRDASKYGLLAQLAVEAGFDWVHYESKSHVHCQKADNSLASRAGGCFPGHATVTLRSGERKGLSELRRGDWVLAVDRSGQVVPTEVLLFLDRDPHRRVPFVAIETESPAHRLLLTPSHLVFAAQAPANGTAGFLPVFARRVRAGDSVLAHRAGGRGLHPVRVLRVSWEEGVGVFAPLTSHGTLLVNGVLASCYAVLESHRWAHRAFAPLRLFHGLLSLLPPSGGAKNCSVPETGVHWYSRLLYQLAWGKADHSVTVEKGGCFPGRAQVHVVGGGRIAMSSLRPGDRVPALSESGRVVPSRVLLFLHRDSESRVRFLVLHTEHGQRLSLTPSHLLFLAPSGAPHPSQFRAQFASRAKQGHYVLVQGDEGGLRPSRIVSISAEEGKGVYAPLTEHGTLFVEGVLASSYAALEEHALAHRAFWPLRFLLSVTQPLQGKRIEGGHSAKEGQGLSENRATHGNLQGLNVTGGNGAFVSARRRRTWPVPSTQGSAEASCEDRERDRGPKVHWYARLLHALGRILKADNSLASRAGGCFPGHATVTLRSGERKGLSELRRGDWVLAVDRSGQVVPTEVLLFLDRDPHRRVPFVAIETESPAHRLLLTPSHLVFAAQAPANGTAGFLPVFARRVRAGDSVLAHRAGGRGLHPVRVLRVSWEEGVGVFAPLTSHGTLLVNGVLASCYAVLESHRWAHRAFAPLRLFHGLLSLLPPSGGAKNCSVPETGVHWYSRLLYQLAWG",
    "response#2": "KSESADASKIGGCFSGSSTVQTLQGQVKMSGLKVGDSVLVVKPDGSLGYSEVILFLDRDENQIRLFQRIVTESGAKITITPSHLIFRSDENRTLDISEGHATFAKYIEIGDYLYVKRDTAAGSRISLERVIAVESGSEKGSFAPLTKEGNVVVNDVLASCYALLDDQSLAHFAFFPVRFMSDIEHLALNLLEKLPFSQMKEIISSHFEFLPAKFTSNMKHAMFHMLEKFHFVKLMPKNVTSQKGMHWYPELLYKMSYYLCFTAETTVQTSRGTLLRLEELRVGERILALDTTTGSFKYSEVILFLDRDPEQRREFLRLRTASGRTLTVTPSHLLMSQKADNSLASRAGGCFPGHATVTLRSGERKGLSELRRGDWVLAVDRSGQVVPTEVLLFLDRDPHRRVPFVAIETESPAHRLLLTPSHLVFAAQAPANGTAGFLPVFARRVRAGDSVLAHRAGGRGLHPVRVLRVSWEEGVGVFAPLTSHGTLLVNGVLASCYAVLESHRWAHRAFAPLRLFHGLLSLLPPSGGAKNCSVPETGVHWYSRLLYQLAWGKADHSVTVEKGGCFPGRAQVHVVGGGRIAMSSLRPGDRVPALSESGRVVPSRVLLFLHRDSESRVRFLVLHTEHGQRLSLTPSHLLFLAPSGAPHPSQFRAQFASRAKQGHYVLVQGDEGGLRPSRIVSISAEEGKGVYAPLTEHGTLFVEGVLASSYAALEEHALAHRAFWPLRFLLSVTQPLQGKRIEGGHSAKEGQGLSENRATHGNLQGLNVTGGNGAFVSARRRRTWPVPSTQGSAEASCEDRERDRGPKVHWYARLLHALGRIL",
    "response#3": "CGPGPNYGVRPKGRRVTPMAYKQFVPNFSENNLGASGRSEGKITRNSERFNELVCNYNPDVIFKDEENTKADRFMTKRCKDCLNVLAIAVMNQWPGVRLRVTEAWDEDGHHPPNSLHYEGRAVDITTSDRDASKYGLLAQLAVEAGFDWVHYESKSHVHCR"
  },
  {
    "reference": "MGNFRGHALPGTFFFIIGLWWCTKSILKYICKKQKRTCYLGSKTLFYRLEILEGITIVGMALTGMAGEQFIPGGPHLMLYDYKQGHWNQLLGWHHFTMYFFFGLLGVADILCFTISSLPVSLTKLMLSNALFVEAFIFYNHTHGREMLDIFVHQLLVLVVFLTGLVAFLEFLVRNNVLLELLRSSLILLQGSWFFQIGFVLYPPSGGPAWDLMDHENILFLTICFCWHYAVTIVIVGMNYAFITWLVKSRLKRLCSSEVGLLKNAEREQESEEEM",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The intended protein structure should align with helix-strand-helix-helix-helix-helix-helix-helix criteria.2. The designed protein should be expressed specifically in the membrane The designed protein sequence is ",
    "response#1": "NLLPSQEQLKSVSTLMVAAKVPAASVTTVALELVNFCYDNGSSAYTTVTGPSSIPEISLAQLASIVKASGTSLRKFCRYFAPIIWNLRTDKMAPANWEASGYKPSAKFAAFDFFDGVENPAAMQPPSGLIRSPTQEC",
    "response#2": "NLLPSQEQLKSVSTLMVAAKVPAASVTTVALELVNFCYDNGSSAYTTVTGPSSIPEISLAQLASIVKASGTSLRKFCRYFAPIIWNLRTDKMAPANWEASGYKPSAKFAAFDFFDGVENPAAMQPPSGLIRSPTQEC",
    "response#3": "NLLPSQEQLKSVSTLMVAAKVPAASVTTVALELVNFCYDNGSSAYTTVTGPSSIPEISLAQLASIVKASGTSLRKFCRYFAPIIWNLRTDKMAPANWEASGYKPSAKFAAFDFFDGVENPAAMQPPSGLIRSPTQEV"
  },
  {
    "reference": "MDQQLAIIVLAAGQGTRMKSATPKLLHPLAGSPIVAHVLRTAQALDAAHVIAVVRHERDQVAAAVEATLPGTVVVDQDEIPGTGRAVEQAIAVLPDDFAGEVLVLNGDVPLLDADTLAAFLERHRGHAAQASVLSAFYDDPTGYGRVVRDDHGAFDRIVEQKDASDDERAIDEINAGIYAFGAGALRDQLANLTTDNAQGEKYLTDVIQLLRSAGSEVEAVPVSAPWLVAGINDRAQLSETAARLNALIVRGWQLAGVTIEDPATTWIDLDVKLEPDVTIRPGTQLKGATVVATGSVIGPDTTLVDCEVGRGAEVKRTDATLSVIGDGATVGPFSFLRPGTVLGADGKIGAFVETKNATIGEGSKVPHLSYVGDATIGVHSNIGAGSIFANYDGVRKHHSEVGSHVRTGSHGVFVAPVRIADGAYTGAGTVVRKDIPAGALAVNVAPQRNIEGWVRTHRPGTAAAQAAEAADEASGPESHDAAAD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that can perform UDP-N-acetylglucosamine diphosphorylase activity, magnesium ion binding, glucosamine-1-phosphate N-acetyltransferase activity in cytoplasm to support peptidoglycan biosynthetic process, cell wall organization, regulation of cell shape, lipid A biosynthetic process, cell morphogenesis.2. The designed protein should interact with enzymes and substrates within the Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway to enhance or regulate its activity.3. The designed protein should have a high specificity for Mg(2+), UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA binding, with minimal off-target effects.4. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.5. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain. The designed protein sequence is ",
    "response#1": "VVVLAAGAGTRMRSDTPKVLHTLAGRSMLAHALHAVGKAAPAHLVVVVGKDRDRVRPEVERLAAELGRPIVTATQEQQLGTGHAVQCGLTALPPDFAGTVVVTSGDVPLLDADTLAELISTHNTRPTAATVLTTVLDDPTGYGRIVRDADGQVTGIVEHKDATEAQRAIREINSGIYAFDVAVLRDGLGRLRSDNAQGELYLTDVLAIARADGRRVAGHRIDDAWQTEGVNDRAQLSRLGTELNRRLLERWMRAGVTVVDPATTWIDAGVTLARDVVIEPGVQLKGTTSVGEGSTVGPDSTLTNVSIGAGASVVRVHGSDSELGDGVNVGPFTYLRPGTKLGVKAKLGAFVETKAADIGAGTKVPHLTYVGDATIGEHSNIGCSSVFVNYDGVNKHRTVIGSYVRLGADNTFVAPVHIGDGAYSGAGAVIREDVPPGTLAVSAPPQRNIDGWVAEHRPGSPAAAAAEAAATTTGTATD",
    "response#2": "VVVLAAGEGTRMKSATPKVLHDICGRSLVGHVLAAARELEPERLVVVVGHAREEVAGHIAGIDPAVRTAVQEVQNGTGHAVRMGLEKLGGVEGTVVVVCGDTPLLTGETLQELTRTHASDGNAVTVLTATVSDPTGYGRILRDADGQVTGIVEHKDATDAQRAITEINSGVYAFDIAALRSALGKLRTDNAQGELYLTDVLAIARKDGHRVGASVAADHREIAGANDRVQLATLRAELNRRTLDAAMRNGVTVVDPATTWIDVDVTIGRDTVVKPGTQLLGGTRIGGRCEVGPDTTLADVTVGDGATVCRTHASSSVIGDGATVGPFTYLRPGTVLGADGKLGAFVETKNSTIGTGTKVPHLTYVGDADIGEHSNIGASSVFVNYDGETKSRTTIGSHVRTGSDTMFVAPVTVGDGAYTGAGTVVRDDVPPGALAVSAGPQRNIEGWTVRRRPGTPAAEAAAAALAAEPAEPDGSDHPT",
    "response#3": "VVILAAGMGKRMQSALPKVLHPLAGKPLLSHVIDVARTLVPSRLCVIYGHGGAAVLKLLEKQNEGQAAPIVAALQEQQLGTGHAVMQAAPMLDDGVPTLILYGDVPLTSAASLQRLVAAAGADKLAILTVVQADPDGLGRIIRGPDGEVLGIVEHKDATDAQRQIREINTGILVARAADLKRWLGQLDNSNAQGEYYLTDIVARAVQDGVAVRATQPAQLWETLGVNSKVQLAELERIHQLNQADALLQALAAAEAIWQQRARAEAMAEGVTMVAPETVWLAYDTAFGRDVVVEPNVFFGPGVTIEDDVHIRANCYLEGIDGKSRAGLRIAAGAKVGPFARLRPGTAIGRDVHIGNFVEVKNATMEDGSKANHLSYVGDARVGAGANIGAGTIFCNYDGFNKNLCDVGAGVFIGSNSALVAPVKIGEGAYVAAGSTITRDVAPDSLAIARAAQRTIDGWKRPVKKT"
  },
  {
    "reference": "MCPGGKAPRKQLATKAARKNAIVVGAVKKPHRFRPGTVALREIRRYQKSTDLLLRKLPFQRLVREIAQDVKQDLRFQSAAIQALQEASEYFLVGLFEDTNLCAIHAKRVTIMPKDMQLARRIRGERN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that localizes to nucleus and can perform structural constituent of chromatin, protein heterodimerization activity, DNA binding with high specificity and efficiency.2. The protein must exhibit the following characteristics: Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability; Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template; DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling .. The designed protein sequence is ",
    "response#1": "ARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGR",
    "response#2": "KPAIRRLARRGGVKRISSFIYDDSRQVLKSFLENVVRDAVTYTEHARRKTVTAMDVVYALKRQGY",
    "response#3": "ISTGGKAPRKQLAPKAARQSAPATGGVKKPHRFRPGTVALRDIRKYQKSTEILIRKLPFQRLVREIAQDFKTDLRFQSSAVAALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKEIQLARRIR"
  },
  {
    "reference": "MGRVVSYNEDVPRCTFCGKTEHQVRKLVAGPNASICDECIALCVDIISEERVKDAEVNSLSLPKPAQIFDYLNRYVIGQENAKRALSVAVYNHYKRVNMELQESAEQLDGNNGHSGQTSKQAKQSVPTQTRATRRSNDPLADVEVAKSNILLLGPTGVGKTYLAQALARVMNVPFVITDATTLTEAGYVGDDVETVLQRLLQAADGDVSRAQHGIIYIDEIDKIARKSGENTSITRDVSGEGVQQALLKILEGTIASVPLEGTRKHKEQDVAQMDTRGILFICGGAFVGLTDIVRKRLGRRETGFGANWHDADMKDEELLEQVNADDLAEFGLLPEFIGRLPVTSVLKELTVDDLTAILTQPANALIKQYRKLFAVDGVDLQFTEQAIRAIADIAIKQGTGARGLRSIIERTLQDTMFQLPSLDDVRQVIVDKASVEGSSTPKLLREAVDVPQGRLKVAQSVLLDRVRTHEA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must be able to unfolded protein binding. The designed protein sequence is ",
    "response#1": "WQFREKEDLDKWIVTSDKTIGGRSEIFLKMSKNNRSALLYGTLSSEPPQDGDSRQSGYCAMISRIPRGAFERKLSYDWSQFNTLYLRVRGDGRPWMVNIRQDTEFIQRKNQMYSYFMFTRGGPYWQEVKIPFSKFFFSNQGRVRDVQGPLVLDKISSIGFTLSDKVDGPFFLEP",
    "response#2": "WQFREKEDLDKWIVTSDKTIGGRSEIFLKMSKNNRSALLYGTLSSEPPQDGDSRQSGYCAMISRIPRGAFERKLSYDWSQFNTLYLRVRGDGRPWMVNIRQDTEFIQRKNQMYSYFMFTRGGPYWQEVKIPFSKFFFSNQGRVRDVQGPLVLDKISSIGFTLSDKVDGPFFLEYTQKEIFLR",
    "response#3": "QCIFCRYNARGNRSKIIHHLYMIHHLNLGSPDNLVFVTEYIEHLKEKLHRNECIYCEKIFPDRNTLMDHMRKRNHREVNPKNHYYDKFYIG"
  },
  {
    "reference": "MKAEKFALIEKALQDLRGGKIIMVTDDESRENEGDLICAARFATGENINFMARNACGLICMPVSSEIAKKFNFYPMTFQNQDNHETAFTVSVDHVSTTTGISAFERSTTAIALVNPETRSEDFRRPGHMFPLVAKDKGIFERQGHTEATVDLCRLAGLEPAGLCCEIMSADGHMARRPELEEKAKEWNLTLITIADLIEYRKSTEKFIERVAEANLPTKYGDFRLYGFLDTITGEHHEALVMGNVSDGKSVLCRVHSECLTGDTFGSRKCDCGEQFDLAMKKISQEGRGVLVYLSQEGRGIGILNKIKAYSLQDKGMDTVDANLALGLPEDARDYSCGIQILKNLGVSKLRLMTNNPQKIDAMNCKENGIEIEERIPLEIPVQKYDEFYLRTKAKRMGHLLSNV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a high affinity for Mg(2+), GTP, D-ribulose 5-phosphate, Zn(2+).2. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.3. The presence of GTP cyclohydrolase II domains is critical for the interaction of the designed protein with its intended targets.4. A protein with zinc ion binding, GTP cyclohydrolase II activity, GTP binding, manganese ion binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity is needed to enhance riboflavin biosynthetic process in a specific context.5. The designed protein is the optimization of the Nucleophile; for GTP cyclohydrolase activity, Proton acceptor; for GTP cyclohydrolase activity to achieve high catalytic efficiency for GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.6. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.. The designed protein sequence is ",
    "response#1": "EVSMPTKYGDFRIIPFRQKSNGAEHVALIKGEWKPDEEILVRMHSSCMTGDIFGSFRCECGDQLHKSMELIEKAGKGVVVYMMQEGRGIGLMNKIAAYKLQDEGLDTIDANIHLGFQADERDYGVGAQILRDVGVSKMKLLTNNPVKRVGIEGYGLTVTEIIPHPEVAKVQMPTEYGDFDMIGFTTASSRDEIVAIIKGEPRKTENVRIHSSCMTGDIFHSQRCDCGEQLAESMKYINEHGGVILYLPQEGRGIGLINKLKAYELIEQGYDTVTANRALGFEDDLREYKDAAAILKYFGIENVNLLSNNPTKFAGLEDYGVNIARREVSMPTKYGDFRIIPFRQKSNGAEHVALIKGEWKPDEEILVRMHSSCMTGDIFGSFRCECGDQLHKSMELIEKAGKGVVVYMMQEGRGIGLMNKIAAYKLQDEGLDTIDANIHLGFQADERDYGVGAQILRDVGVSKMKLLTNNPVKRVGIEGYGLTVTEIIPQPEVRVAEATLPTKYGGEFKIIVYENEVDDMKHIALVKGDIQPEDEVLVRVHSECVTGDLFGSLRCDCGDQLHKAMEMVGQEGKGVIVYMHQEGRGIGLANKIRAYDLQEQGRDTVEANIELGFKEDLRDYGIGAQILVDLGVRKMRMITNNPKKIVGVEGYGLTVVERVP",
    "response#2": "MELEVSMPTKYGDFRIIPFRQKSNGAEHVALIKGEWKPDEEILVRMHSSCMTGDIFGSFRCECGDQLHKSMELIEKAGKGVVVYMMQEGRGIGLMNKIAAYKLQDEGLDTIDANIHLGFQADERDYGVGAQILRDVGVSKMKLLTNNPVKRVGIEGYGLTVTEIIPHDSVPIGNDERVAEATLPTKYGGEFKIIVYENEVDDMKHIALVKGDIQPEDEVLVRVHSECVTGDLFGSLRCDCGDQLHKAMEMVGQEGKGVIVYMHQEGRGIGLANKIRAYDLQEQGRDTVEANIELGFKEDLRDYGIGAQILVDLGVRKMRMITNNPKKIVGVEGYGLTVVERVPKYPMEEGKTKELRREVEVRMPTEFGEFTMYGYSNRVDAAEHVALVMGKPGPEKPVLVRVHSECLTGDVFTSRRCDCGSQLHAAMRQIAEEGEGVILYLRQEGRGIGLLNKLRAYKLQDEGHDTVEANRKLGFKADLRDYGIGAQILRDIGLRKLRLLTNNPKKIIALDGYGLEVVEREVSMPTKYGDFRIIPFRQKSNGAEHVALIKGEWKPDEEILVRMHSSCMTGDIFGSFRCECGDQLHKSMELIEKAGKGVVVYMMQEGRGIGLMNKIAAYKLQDEGLDTIDANIHLGFQADERDYGVGAQILRDVGVSKMKLLTNNPVKRVGIEGYGLTVTEIIPEYEEVSREVEVRMPTEFGEFTMYGYSNRVDAAEHVALVMGKPGPEKPVLVRVHSECLTGDVFTSRRCDCGSQLHAAMRQIAEEGEGVILYLRQEGRGIGLLNKLRAYKLQDEGHDTVEANRKLGFKADLRDYGIGAQILRDIGLRKLRLLTNNPKKIIALDGYGLEVVER",
    "response#3": "MASGRVPSEHGPFTAITYRSPADDCEHVALVLGEVNQAGAEPGSAESAPVLVRVHSECLTGDVFASRRCDCGEQLAVAMTRIGEAGRGVVVYLRGHEGRGIGLSHKLRAYNLQDAGLDTVDANLAQGLPVDSREYGIGAQILRDLGVREIRLMTNNPAKYRGLAGHGVRVATRVASVRLPTQFGEFVAYGYESLVDGEQHLALVKGEVDGQENVLVRVHSECLTGDVFHSLRCDCGDQLQAALDAIEKEGRGVLLYMAQEGRGIGLLNKLRAYELQEQGRDTVEANLELGFAPDMRDYGIGAQILADLGLSSIRIMTNNPRKLVGLEGYGLTITERVPLPQQVQARIPTIYGDFTGHVFRDFDGTEHLALVYGQIMGSEPVLVRVHSECLTGDILASMRCDCGGQLHTALEAIAEEGAGVLVYLRGHEGRGIGIGHKIAAYSLQDEGHDTVDANIELGLPVDSREYGIGAQILVELGITKMRLITNNPAKIGGLAGYGLEIVERRVAEATLPTKYGGEFKIIVYENEVDDMKHIALVKGDIQPEDEVLVRVHSECVTGDLFGSLRCDCGDQLHKAMEMVGQEGKGVIVYMHQEGRGIGLANKIRAYDLQEQGRDTVEANIELGFKEDLRDYGIGAQILVDLGVRKMRMITNNPKKIVGVEGYGLTVVERVGVDLPTDFGDFELIAFRQTNTQELHLALIKGKWDKDEPILVRVHSSCMTGDIFGSCRCDCGAQLHGAMQMVQKEGKGVILYMNQEGRGIGLINKLKAYKLQEEGMDTVQANLALGLPSDSRDYGVGAQILRDLEVSKLRLISNNPQKRVGLLGYGLQIVEQV"
  },
  {
    "reference": "MKTLILLLLLFNLTLYASATDSDEYDHCNCDDEEALFSIHTILVGQKVSDFFIAIAYFSIPIELLYFVSRSNVPFKLLFLQFIAFIVLCGMTHLLNAYSYHSHPSFQLLLSLTVAKFLTALVSCATALTLPPLIPLLLRIKVREIFLRQNVMELGQEVGIMKKQKEASWHVRMLTREIRKSLDKHTILYTTLVELSKALDLHNCAVWMPNDDRREMYLTHELKSDSVKSFHHNSIPVIDPDVLEIRKTKGVRILRPESKLGAASSGGGSTEELGAVAAIRMPMLHVSNFKGGTPELVDTCYAILVLVLPGSNSRVWTAPEMEIVEVVADQVAVALSHASVLEESHLMREKLEEQNRALQQSQKNAMMASQARKSFQTVMSHGLRRPMHSVVGMLSLFQEGNMRPEQKIIGDTMLKVGNVLSSLVNDVMDISENKKGGLQLEMKPFLLHSMLREAACIAKCLCVYQGFGFQIDVEKSLPEKVLGDEARTFQVIMHIVGYLLNTRDRGNLVFRVFVTSESGDKDDKKFGVWRSSSPVEYVDIKFDFQITGSSQSDEPISTKQQIGRRLNHNSESKEGLSFNMCRRLVQIMQGNIWILPNSQGLVQGVSLLLKFQIGPSLERYMFAPKDYSNSQFGGLKILLADDDGLNRIVTKKLLERLGCLVTAVSSGFECLGAISGSGSNSFKIIMLDIHMPEMDGFEVAARIRKFNGPNWPLIIALIANAEEQVKDRCMLAGMNGVIRKPILLHQIADELRSVLLRAGEKL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a low Km value for ATP + protein L-histidine = ADP + protein N-phospho-L-   histidine..2. The designed protein should have a high specificity for Cu cation binding, with minimal off-target effects.3. Ensure the presence of a signal peptide within the protein.4. Design the protein with a signal peptide incorporated.5. The protein should contain a Helical membrane-spanning region.6. The stability of the protein-Cu cation complex should be optimized to maximize enzymatic activity.7. The designed protein have Histidine kinase, Response regulatory domains with specific sequence motifs that are crucial for its activity.8. The designed protein should have ethylene binding, protein serine/threonine kinase activity, ethylene receptor activity, metal ion binding, phosphorelay sensor kinase activity to facilitate negative regulation of ethylene-activated signaling pathway. The designed protein sequence is ",
    "response#1": "DRHKILYTTLVELSNTLGLQNCAVWMPNVEKTHMILTHELNTRSVSCSIPITDPDVKRIKENNEACILDSNSILATASHGDSGAAVVGLGPVAAIRMPMLRVNDFNGGTPELRQTCYAILVLILPMTEDRVWSFFELETIKVVADQVAVALSHAAILEESLDRHKILYTTLVELSKTLGLQNCAVWMPNVEKTEMNLTHELNGRNVNCSIPITNPDVVRIKGSDEVNIIDSDSILATASSGVYGGAGPVAAIRMPMLQVCNFKGGTPELRQTCYAILVLTLPSAEPRSWGAQELEIIKVVADQVAVADKHTILYTTLVELSKTLDLHNCAVWMPNENRTEMNLTHELKPSAKPYHFSILVNDPDVLEIKGSKGVKILRSNSALGAASGGGSEEAGAVAAIRMPMLRVSNFKGGTPELVDTCYAILVLVLPSMNSRGWSFDEMEIVEVVADQVAVALSHASVLEESLQGGVMSDGMRRPMHSILGLLSMMLDENMSSEQRTIVNTMVRTSSVLSTLVNDAMDNARKDSGRFPLEMRSFHLHSMIREAACLAKCLSVYNGFGFAIEVEKSLPDHVTGDEKRVFQVILHMVGNLLDVNNGGEFVILRVFSESRSQGRNDHIWSTWRHSSSDGYVYIRFEIAIKNCSSQSEGSVSTAQTGGRRYASDGLEESLSFSICKRLVQLMQGNIWVIPHPQGFAQSMALVLRRAQ",
    "response#2": "VMNHEMRTPMHAIIALSSLLQETELTPEQRLMVETILKSSHLLATLINDVLDLSRLEDGSLQLEIATFNLHSVFREVHNLIKPVASVKKLSVSLNLAADLPVQAVGDEKRLMQIVLNVVGNAVKFSKEGSISITAFVAKSESLRDFRAPEFFPAQSDNHFYLRVQVKDSGSGINPQDIPKLFTKFAQTQSLATRNSGGSGLGLAICKRFVNLMEGHIWIESEGPGKGCTAIFIVKLGFADRHTILKTTLVELGRTLALEECALWMPTRTGVELQLSYTLRHQNPVGLTVPIQLPVINQVFSTNRAVQISPNSPVAKLRPTGKYMPGEVVAVRVPLLHLSNFQINDWPELSTKRYALMVLMLPSDSARQWHVHELELVEVVADQVAVADRHTILKTTLVELGRTLALEECALWMPTRTGVELQLSYTLRHQNPVGLTVPIQLPVINQVFSTNRAVQISPNSPVAKLRPTGKYMPGEVVAVRVPLLHLSNFQINDWPELSTKRYALMVLMLPSDSARQWHVHELELVEVVADQVAVADRHTILKTTLVELGRTLALEECALWMPTRTGLELQLSYTLRQQNPVGYTVPIHLPVINQVFSSNRALKISPNSPVARMRPLAGKHMPGEVVAVRVPLLHLSNFQINDWPELSTKRYALMVLMLPSDSARQWHVHELELVEVVADQVAVALSHAAILEESL",
    "response#3": "MKKAISIFPKEFGFFPYIFLVYTIMPFLSLLKESGVKQGIGYGMLLLFVAAYRQLFCSVGKASFTYWLIVQMAVILMYSVFYNITYIYLGFFPANFVGYYKEKTNFNRAFCALIFILLFPCLYQFIANSVSLRELFSVLPFLVIMLIYYQDQITKNPAGGGDEKRLMQIVLNVVGNAVKFSKEGSISITAFVAKSESLRDFRAPEFFPAQSDNHFYLRVQVKDSGSGINPQDIPKLFTKFAQTQSLATRNSGGSGLGLAICKRFVNLMEGHIWIESEGPGKGCTAIFIVKGDEKRLVQIILNVVGNAVKFSKEGNISITAFVAKSESLRDPRAPDFFPICGENQFYLRVQVKDSGLGINPQDIPRLFTKFAQTQPVATKNSGGSGLGLAICKRFVNLMEGHIWIDSEGPGKGCTATFVVKISNISIPSAIDQRTFEEYTERTNFERPLTSGVAYALKVPHSEREKFEKEHGWAIKKMETEDQTVVQDCVPENFDPAPIQDEYAPVIFAQETVSHIVSVDMMSGEEDRENILRARASGKGVLTSPFKLLKSNHLGVVLTFAVYDTSLPPDATEEQRVEATIGYLGASYDMPSLVEKLLHQLASKQTIAVDVYDTTNTSGLIKMYGD"
  },
  {
    "reference": "MNTKYIFVTGGVVSGLGKGISAASLGRLLKARGYKVTSQKLDPYINVDPGTMSPYQHGEVFVTDDGLEADLDLGHYERFIDENLHTYSNLTTGKVYWNVINKEREGKYLGETVQVIPHVTNEIRNFVIQAGKHAQADVVITEIGGTTGDMESLPFLESIRQVPLEVGRNNCLFLHVTYIPYISGSKEYKSKPTQHSVKELQSHGINPDMIIARCDEKIPQSVLDKISLFCNVEKDCVIENTTLDSLYEVPLMLEEQRMADIVCEKLDLEVRKPDLENWKNMVEHIHNSKESVTIALVGKYVQLHDAYLSVSEALYHAGFQFETKVDICWVDSETLEDQDLDVVFEGVDGIILPGGFGGRGVEGMIKVANYAKHHQIPYLGICLGMQIAAIAFARDALGYADANSYEFDPKSTHTIIDFMEDQSDTIKKGGTMRLGAYPCAIHPNSMLSKYYEKDLIEERHRHRYEFNNLYRAEFEQNGMMITGNSPDKQLVEAIEYKDHPFYVGVQYHPEFKSRPNRAHPLFVGLIENSIQYKKEKENADQ",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein with the reaction H2O + L-glutamine = L-glutamate + NH4(+), the Nucleophile, Nucleophile; for glutamine hydrolysis must be optimized to ensure efficient catalysis.2. The designed protein should be optimized for CTP synthase activity, metal ion binding, glutaminase activity, ATP binding activity in order to improve glutamine metabolic process, 'de novo' CTP biosynthetic process.3. We require a protein that can specifically bind to the metabolites produced in the CTP from UDP: step 2/2, CTP biosynthesis via de novo pathway, Pyrimidine metabolism pathway.4. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.5. The protein contains novel CTP synthase N-terminal domains that confer a unique function or activity.6. The designed protein should have a high specificity for CTP, L-glutamine, UTP, Mg(2+), ATP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "KHIFVTGGVCSSLGKGLTCASIGMLLERRGLRVRLQKFDPYINVDPGTMSPYQHGEVYVLDDGSETDLDLGHYERFTSAPLDRNCNFTTGRIYLSVIQKERRGEYLGKTVQVVPHVTDEIQSAIRAVVAPDVDVVITEIGGTVGDIEGLPFLEAIRQFALQVGKQNCLFIHLTLVPYLRAARELKTKPTQQSVARLREIGIQPDILICRSERPLSKEDKQKIALFCNIEPDAVIEELDKTYSIYEVPISLSENQLDELIVHKLGLPTGKPDLSDWEGLVDKIKSPKQEVTIALVGKYVSLHDAYKSIVESFIHAGAANEVKVNVKWIYSGDVNESNAASLFKDVDGILVAPGFGERGIEGKITATKYAREHGIPFLGICLGMQMAVVEFARDVLGLKDAHSKEINPETPYPVIDLMESQKNVTNKGGTMRLGAWQCSVEKDSRAYSIYGTDSIMERHRHRYELSSDYIEALDKAGMKATGRNAETGLVDIVEIPSHPWFVAVQFHPEYRSTVENPHPLFVDFVKAALDHAS",
    "response#2": "MAKYIFVTGGVTSSLGKGIISASLARLLQARGYSVTIQKLDPYINVDPGTLNPYEHGECYVTTDGAETDLDLGHYERFLNVETSQANNVTTGRIYQSVIEKERRGEFLGKTVQVIPHITNEIKQRIQILGNTGKYDIIITEIGGTVGDIESLPYIEAVRQLKWELGPHNTLVIHLALVPYLAAAGELKTKPAQHSVKSLMQAGVQAGVLVCRTEHHLPQEVKNKLAQFCNVRPAAVIESIDAETIYDVPILMEQQQLDRVVLEDLGLESPAADMSQWVALLERLRNPQHELKDSYISINEALIHAGAANHLAVDVKWIHSESLNQENVSAILSELDGILVAPGFGERGIEGKITAVKYARENKVPFFGICLGMQCAVIEFARNVLNLSDANTTEIDVNTKDPVIFLMEKQKEITDKGGTMRLGAYDCVLDTGSKAHAAYGKAKISERHRHRFEFNNQYTEDFKKAGMNAVGSNPEGGMVEIVEIENHPWFVGVQFHPEYKSTVLNPHPLFVDFIKAVIEAKG",
    "response#3": "YLFVTGGVVSSLGKGITSASIAVLLEKRGLKVSLQKLDPYINVDPGTMSPFQHGEVYVTEDGAETDLDLGHYERFTHAAVNKYSNATTGSIYSEVIRRERRGDYLGRTVQVIPHITDHIQELILMGAQQGPDVAIVEIGGTVGDIESLPFLEAIRQFGLKQPAGDVCYVHLTLLPFLRASGEMKTKPTQQSVGKLREIGIIPNILVCRCERPMEPGMREKISLFCNVRPEDVIEEVDVDTSIYEVPLNLLRDGLDAALVNRLGLECNKPDLTDWTALLQRHYNPQHELQDAYKSIYESFIHAGAANDCRVEVVPIHSEDLEAPDADPEKIFKDIHGILVAPGFGERGIEGKLRAIRYAREKKLPFFGICLGMQCAVVEFARHVMGLKEAASTEMNAETPDPVIALMKEQKKITQKGGTMRLGAYACVLAEDSLAAKAYGATQISERHRHRYEFNNAYREQMDAAGLKATGINPESGLVEIVELRDHPWFVGVQFHPELKSTVENPHPLFVAFVKSAAEVQ"
  },
  {
    "reference": "MTRFIFITGGVVSSLGKGLASAALGAALQARGFKVRLRKLDPYINVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTCVSSRQSDNVTTGRIYSDVIAKERRGDYLGATIQVIPHITDAIKEFVLGNIEDEDFVLCEIGGTVGDIEGTPFLEAIRQLGNELGREQVMYLHLTLLPWIATAGELKTKPTQHSVKELLSVGIQPDILLCRAEREIPQSERRKIALFCNVRPDAVIPALDVDSIYRVPISYHEQGLDAQVCRHFGLTTPEPDFSRWQDIVDRATHPEGEVTIAVVGKYTGLLDAYKSLSEALTHGGIANNVKVRLNWIDAEIFEREDVIKHLESVHGILVPGGFGERGTEGKIAAARFARERQIPYFGICLGMQMAVIEAARHRSGIAGAGSSEFGTPGEPVVGLMTEWAKGEDIEKRASDGNLGGTMRLGAYPCVLTPGSKVREIYGQDHISERHRHRYEVNTAYKERLEAAGMVFSGMSPDGVLPEIVELPDHSWYIAVQFHPELKSRPFEPHPLFVSFIGAAVQQSRLV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Ideal protein should have a binding affinity for key enzymes in the Pyrimidine metabolism pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "PLCLGIDPHPELLRAWGLGTDAAGLAGFSDICVAAFAGFAVVKPQVAFFEAYGSAGYAVLERTIAALRESGVLVLADAKRGDIGSTMAAYASAWAGDSPLAADAVTASPYLGFGSLQPLLDTAREHDRGVFVLAATSNPEGASVQCAVAPGAIPGRTVAQAIVDAAAEVNAGAARGSVGVVVGATLSAPPDVSALNGPVLVPGVGAQGGRPEALAGLGGARRGQLLPAVSREVLRAGPDVAALRR",
    "response#2": "LPDAYKSIIEAFIHAGAQNECKVTVKWIQSEHINEENVGTMLQHMNGVLVAPGFGERGFHGKIETIKYVRENNIPFLGICLGMQCAAVEFARNVLGLAGAASTEMDASTPHPIIDMMAEQKEITQKGGTMRLGAYTCELKKGSKAYSIYGKSKISERHRHRYEFNNSYLPQFQEAGMVASGTNPETGLVEIFELQNHPWFVAAQFHPELKSTVLTPHPLFVKFIVAR",
    "response#3": "PLCLGIDPHPELLRAWGLGTDAAGLAGFSDICVAAFAGFAVVKPQVAFFEAYGSAGYAVLERTIAALRESGVLVLADAKRGDIGSTMAAYASAWAGDSPLAADAVTASPYLGFGSLQPLLDTAREHDRGVFVLAATSNPEGASVQCAVAPGAIPGRTVAQAIVDAAAEVNAGAARGSVGVVVGATLSAPPDVSALNGPVLVPGVGAQGGRPEALAGLGGARRGQLLPAVSREVLRAGPDVAALRR"
  },
  {
    "reference": "GCCGSDIKQYVFGGWAWAWWCISDTQRISLEIMTLQPRCEDVETAEGVALTVTGVAQVKIMTEKELLAVASEQFLGKNVQDVKNVVLQTLEGHLRSILGTLTVEQIYQDRDQFAKLVREVAAPDVGRMGIEILSFTIKDVYDKVEYLSSLGKTQTAVVQRDADIGVAEAERDAGIREAECKREMLDVKFMADTKVADSKRAFEMQKAAFSQEINVKTAEAQLAYELQGAREQQKIRQEEIEIEVVQRRKQIAIEEKEVIRMDKELFAMVKRPAEAEAHRMQQIAEGDKVKQVLIAQAEAEKIRKIGEAEAFVIEAIGKADAEKMKLKAEAYQQYGEAAKMALVLDSLPKIAAKVASPLAKVNEIVILSGDNNKLTSDMNRLLAEIPVSVHALTGVDLTKIPLIQKATGGQA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein have Band domains with specific sequence motifs that are crucial for its activity.2. The designed protein should have a specific function within the flotillin complex. The designed protein sequence is ",
    "response#1": "DPAWEKQQRKVSRRLWSVCYYKWVCTQIENIEEDFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNKALDYIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFR",
    "response#2": "DPAWEKQQRKVSRRLWSVCYYKWVCTQIENIEEDFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNKALDYIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFR",
    "response#3": "MYFRREELAFARYFIGLISIVCLSATLFTFLTFLIDVRRFRYPERPIIFYAVCYMMVSLIFFVGFLLEDRVACNAARPAQYRAATVTQGSHNKACTLLFMVLYFFTMAGSVWWVILTITWFLAAVPKWGSEAIEKKALLFHASAWGIPGTLTIVLLAMNKIEGDNISGVCFVGLYDVDALRYFVLAPLCLDVAVGVSLLLAGIVSLNRVRIEIPLEKENQDKLVKFMIRIGVFSVLYLVPLLVVIGCYFYEQAHRGVWETTWVRERCRDYHIPCPYQMPQMSRPDLILFLMKYLMALIVGIPSVFWVGSKKTCFEWASFFHGRRKEFIFLIDCSGSMSGEPIKKAKRALEIIIRSLNENCKFNIYCFGSRFTKAFDNSKMYNDETLKEISGYVEKIDADLGGTELLPPIRDILSTESDFEYPRQLFILTDGEVSERDSLINYVATESNNTRIFTYGIGNSVDTELVIGLSKACKGYYEMIKDNSNFEEQVMKLI"
  },
  {
    "reference": "MRKGRNIVLGITASIAAYKGVEIARVLMKKGYRVRCVMTESASRFITPLTFEAITGEKVVATFWEESKVINHISLAKWADLVLVAPATADFLAKISNGFADTPLSALLLATKAPILLAPAMNSGMYENSVTQANITRLKERGIFFVGPAKGELACRTYGLGKMSDVKEIVLITEKLLSSQCLRDIRVLVTAGPTREPIDPIRFISNRSSGKMGVSIALQAFKEGADVLLIHGKLSIRIPSLIRSVQIETAEELKGAILHSLNKKEWRPDIVIMTAAVCDLKPKNFSSEKIKKEKHLCSIELSRTPDILKTMAEVKPEERPFIVGFAVETDKEKIKKEAKRKLFEKKLNMVVANLAEDAFEKETNTVWITTEEEEQLKSGYKRKVAEEIIKEIAKKYKEEKAKGINE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. We require a protein that can specifically bind to the metabolites produced in the Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway. The designed protein sequence is ",
    "response#1": "MRIVLGVSAGIAAYKAVILARLLTEDGRQLEVIPTPAALEFVGRATWEAITGRPVTTSVFEGVDEVRHVRLGQQADLIVVAPATADLLARTRAGTAGDLLSSTLLASEAPKLLVPAMHTEMWRNPATVENVEVLRGRGIEVMEPASGRLTGTDSGPGRLPEPEQIKARIDQARA",
    "response#2": "IIGVAGSVAVGKSTTARVLQALLARWEHHPRVDLVTTDGFLYPNAELARRNLMHRKGFPESYNRRALMRFVTAVKSGADEAAAPVYSHLLYDIVPGEYQIVRHPDILILEGLNVLQTGPALMVSDLFDFSVYVDARIEDIEQWYISDD",
    "response#3": "IIGVAGSVAVGKSTTARVLQALLARWEHHPRVDLVTTDGFLYPNAELARRNLMHRKGFPESYNRRALMRFVTAVKSGADEAAAPVYSHLLYDIVPGEYQIVRHPDILILEGLNVLQTGPALMVSDLFDFSVYVDARIEDIEQWYISEVL"
  },
  {
    "reference": "MKKILSTSALKQLDLATIHNQEISSWELMERAAFAAFQAIFHDYPDILQAEVSILCGKGNNGGDGLAMARMMRGVGIKVQVFLLESVDYSADNQENQKLLDEFQKVTLNEHLVLNTSTYLIDCLFGFGLNKGLDHSWKYLIEQINAFEGTVLSIDMPSGLPVDFPLEENPIVVEADRVYSFELPKLSFFMPQNGRYVLDFTLIPIQSDQATLEDLSSNYYYLEKEDIRKLLKNRSKFSHKGIYGHGCIIGGSLGKMGAVILASRAALRTGCGLVSNLTPTCGHQILQISHPETMVIMDSGEENLRDFQLPQSYQAIAVGIGMGTSEETERGLGLFLQSLDKDQALVLDADALNLLALNPHWLGHLPENSILSPHPKELKRLIGEWDSDEDKLMKAVDFAIAHKVVLLIKGANTAVINSEGTVYFNSSGNPGMATGGTGDVLTGILAGLKAQGYTALEAALIGVYAHGLAGDLASTELSEEGMIAGDLINYLPKVWNIVRQD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.2. The protein should have a high affinity for (6S)-NADPHX, AMP.3. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.4. The protein must be able to metal ion binding, ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding, NADPHX epimerase activity. The designed protein sequence is ",
    "response#1": "MAEADRLAIAAGPLDGYGLMRRAGEAVAAVVLARYPAAKRVHVLCGPGNNGGDGYVVARLLQESGVDVALWGSGESRAGSDAALAAADCRLEPRPLSAFVAAPGSLVVDALYGAGLSKAPSGDAARAIEIATDHGLPVVAIDLPSGVSGESGAVLGSAFLAAVTVTFARKKPGHLLLPGRAQCGEVLLADIGIGDGTIPAFLTAESIALLLRRRDRQAHKGHFGHCLIVAGSSGKTGAAALSANSAVRAGSGLVTLAVPESLNPILEVKTTEAMTVPLADSGGGYLTDSAFPAIAGLLPDKDALAIGPGLGQHPATVTLVRALLKAADLPLVIDADGLNAVAQNANCLLDKKSTHVVLTPHPGEMARLMGATIADVEADRIATARKFAAAFGVHLILKGSRTIIAAPDGSTAINGSGNPGMASGGMGDVLTGIIVSLLGQGYNCHDACRLGVFIHGYAADLVAQDKGEIGMCAGDVQEKLPYAYAHKGDAGRAVICAGSPGFTGAAALSSYAAVKAGAGLVALYTPLSSRDVLAGKLTEVMVHGLLERMPGILGGGATGDVVKNANAADVLAIGPGLGTSESTQQVVRDILLQVETPVVIDADALTALQGHTEILTQMQAAKVLTPHPGEMARLIGKEISEINADRINIAAQYAQEWNAVLVLKGAPTVIACPDGTVYVNSTGSSALATGGSGDVLTGIIAGLAAQEITLQEAAVCGVYLHGKAADR",
    "response#2": "MVLWPPGCSARPXFALAPQETLSTLAKAAYEKMPPEVSVLASGDSQALDSAFDDAELIIDAIFGFNLKGAVRGAAVDVIDKINESHKPVLAVDMPSGLEADTGKVHGRAVKADATVTFTAPKVGTMLYPGLELTGAPIVADIGIPEELIDENWGKAALAPRRSTAHKNDFGHVLAVAGSEGKAGAATLVVLAALRAGAGLVTLAARPEVLRTALPGVPEAMGVPLPGNGPLGPGDLAPLRAALRGKTAFAMGPGIPRGPQTGQLLGELLAALDPGCATVVDADALNALAEERDAIAERLRKAPVRPVLTPHPGEFSRLTGEEVERVESDRIDAAVRASSRFGACVVLKGARTVVADPEGTSAVCAHGNPGMATAGAGDVLTGITAAVLARRSGGGSTGERARLAVFLHALAGDLAARRTGETGLVASDIAEVGLPRVFRRWDESGMRELDRRAMARPGVGGGALMERAGAALLQALEETFPDARRLGVLCGPGNNGGDGYVLARLAGASGWTVKVHGSAGGTGTASDGTRAAEAWRAVGGSVDPLEDFAPEAADVWADCLFGIGLGRPIDGALAALIEHLNATGRPVLAADVPSGIDVNTGSVQGVAVRAALTVTMIADKLGLHTGPAVDYVGAVRVAGLDVAGSDKYSRGVVGIATGSATYPGAAVLSVTGALAGPTGMVRYAGSADRDVVHRHPSVVVAPRVADAGRVQAWVCGSGLGTDDGARTELRSVLATSLPVLLDADALTLLVGGQHATDLRRDAPLVITPHDGEFARLAGERPGADRVGAACRLAAWINAVVLLKGDRTIVATPSGEAWANPTGSPALATAGSGDVLAGLLGSLLAAGVPPERAAIMAAYVHGLAGRRAERDGPVTSPDVAAAL",
    "response#3": "MGEADRLTIASGIAGLDLMEAAGRGVAEAVRTRYPDARQIVVLCGPGNNGGDGFVAARHLAEKGLDIRLALLGDRAELKGDAAEMARRWTGPEHAFEENILEGADVVVDALFGAGLTRPIDGKTALMIEAVTTRNIPVIAVDVPSGIDGTSGEIKGTAFEAAATVTFFRRKPGHLLFPGRKCCGPVETIDIGIPDRVLDAADIASWWPRADAGSDKYSRGVVGIATGSATYPGAAVLSVTGALAGPTGMVRYAGSADRDVVHRHPSVVVAPRVADAGRVQAWVCGSGLGTDDGARTELRSVLATSLPVLLDADALTLLVGGQHATDLRRDAPLVITPHDGEFARLAGERPGADRVGAACRLAAWINAVVLLKGDRTIVATPSGEAWANPTGSPALATAGSGDVLAGLLGSLLAAGVPPERAAIMAAYVHGLAGRRAERDGPVTSPDVAAALRPVLAEAPALWLPGFPVPAVDAHKYRRGHASIFSGGPAATGAARLSALAAARSGAGAVTVLSPANAVQVNAAHLTSIMLRKADTAADLGAFLTDRRPSSFVLGPGFGIGEKARDFALALLGADHARDSGVEGLVLDADGITSFRSEPETLFQAARRSGAPALVMTPHEGEFGRLFPDIANDKALSKLARARAAAARASAVIIYKGADTVIAAPGGRAAINSNGAPWLATAGSGDVLAGIIAGLLAQGMPAFEAACAAVWIHAEAGSRFGPGLIAEDLPLAMVPVLREYTGPGDLLGLDRDPDSYKGDNGEVLVVGGGPYAGAPTLSALAALRAGADLVRVACPGSVASEVQGFSPNLIVRPLPGDRLGPSHAERIASLAAGSDAVVLGPGLGDGDGTEKFVREFLTGYGGRAVVDADALGVVPEVDTDADLICTPHRGELVNMGGATVADPDERAELVREFAAELGHALLVKGSVDVISDGDEIRLNRTGNPGMTVGGTGDVLAGTVGALAAVTDPFRAAAVGAYAVGRAGDAAAGANGTGLVATDLPDRLPEAMRN"
  },
  {
    "reference": "MTVKGIQKNWQHLWKWGTLILGLVIICSASNNMWVTVYYGVPAWEEADTILFCASDAKAYSTEKHNVWATHACVPTDPNPQEITLENVTENFNMWKNHMVEQMHEDIISLWDESLKPCVKLTPLCVTLYCTNVTKNNSTGNNSSTVEDREEMTNCSFNVTTEIRDKQKKEYALFYKLDVVQIDGSNKTYRLINCNVSTIKQACPKVTFGPIPIHYCAPAGFGILKCRDKNFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEGKMIIKYENLTDNTKTIIVQFNKSVEIRCTRPNNNTRKSISFGPGQAFYATGDIIGDIKQAHCNVSKTDWNKTLQEVATKLKELFNKNITFNSSAGGDLEITTHSFNCGGEFFYCNTSELFTNTSTKNGTNETPTLPCKIKQIVRMWQRVRQAMYALPIAGNITCTSNITGLLLTRDGNNDSHTETFRPGGGDMRDNWRSELYKYKVVKIKPLGIAPTWARRRVVGREKRAVGLGAVFLGFLGTAGSTMGAASITLTVQVRQLLSGIVHQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLALERYLRDQQLLGLWGYSGKLICPTNVPWNASWSNKTYNEIWDNMTWIQWDREIYNYTQQIYSLIEESQSQQEKNEQDLLALDKWTSLWSWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSIVRRVRQGYSPLSLQTLIPNPTGADRPGEIEEGGGEQGRTRSIRLVNGFLALAWEDLRNLCLFSYHQLRDLVLIAARTVET",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The presence of Retroviral envelope protein GP41-like domains is critical for the interaction of the designed protein with its intended targets.2. For general function, the protein need meet that The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Transmembrane protein gp41: Acts as a class I viral fusion protein; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain3. The protein should have structural molecule activity and be active in host cell endosome membrane. The designed protein sequence is ",
    "response#1": "MRVMGIKKNWQRWWIWGILGFWMLMICNVVGKLWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQEMILENVTENFNMWRNDMVEQMHEDVISLWDQSLKPCVKLTPLCVTLNCTDVKNTTGANSTGWGKEEIKNCSFNITTELRDKRQKVYSLFYRLDVVPIEEGQGNSSNSGYKEYRLINCNTSAITQACPKVTFNPIPIHYCTPAGYAILKCNEETFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVIIRSENITNNVKNIIVQLAEPVRINCTRPNNNTRTSIRIGPGQTFYTSNIIGDIRQAHCNVSRAEWNKALNKIGKQLRKYFVNKTIKFANSSGGDLEITTHSFNCEGEFFYCNTSGLFNGTWNASMQESNSTESNETIILPCRIKQIINMWQRVGQAMYAPPIQGIIKCVSNITGLILTRDGKSSNSTDEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRAVGREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNASWSNKSQKEIWENMTWLQWDKEISNYTDIIYHLIKESQNQQEKNEQDLLALDKWADLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSVINRVRQGYSPLSFRLTPNPRGPDRLG",
    "response#2": "MRVMGTQRNYPPFWRWGTLILGLVIICSASNNLWVTVYYGVPVWKDTKTTLFCASNAKAYDTEVHNVWATHACVPTDPNPLETPLVNVTENFNAWKNDMVEQMHEDIISLWDQSLQPCVKLTPLCVTLDCVEYKHKHNASNSTVSTITVSNSNELLNCSFNMTTILRDKKEKMAALFYRLDVVPMEGQNSSKNSSYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCNDTKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEGEVVIRSQNITDNSKTIIVQLTEPVNITCIRPNNNTRKSWHIAPGQAFYATGEVIGDIRQAHCNVSRTKWNETLYKVATQLRKHFKNTTIIFANLSGGDLEVTTFTFNCGGEFFYCNTSALFNSSWNSTKNNTDIACNITLPCRIKQIINMWQRVGQAMYAPPIPGVIRCVSNITGILLTRDGGANGTNNETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPSRARRIVVERGKRAVGIGAMIFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAMERYLRDQQLLGIWGCSGKLICTTAVPWNSSWSNKSQKDIWENMTWMQWDKEISNYTNTIYELLEESQNQQEKNEKDLLALDKWQNLWSWFDITHWLWYIKIFIMIVGGLIGLRIVFAVLAVVNRVRQGYSPLSFQTPFHQQREPDRPEEIEEGGGE",
    "response#3": "MRVMGIQKNYPLLWKGGMMIFWIMTICSATNYLWVTVYYGVPVWKDAETTLFCASDAKAYETEKHNVWATHACVPTDPNPQEIHLENVTEDFNMWKNSMVEQMHTDIISLWDQSLKPCVKLTPLCVTLNCSNVNGNSSTNMVEEMKNCSFNMTTELRDRKERVHALFYRLDIVPLKENDGTYRLINCNTSAITQACPKVSFDPIPIHYCTPAGFALLKCNNKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSKNITDNAKTIIVQLNKSVEINCTRFNNNTRKSVRIGPGQTFYATGEIIGNIRQASCTLNETEWNKTLKEVRDKLRRHFPNRTISFANHSGGDLEITTHSFNCRGEFFYCNTSSLFNRNITEKITDNNSSITITCRIKQIVNMWQGVGRAIYAPPIEGVITCRSNITGLLLTRDGGNTNNTTETFRPGGGDMRDNWRSELYKYKVVEIKPLGIAPTKAKRRVVEREKRAVGLGAMFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNASWSNKSQKEIWENMTWLQWDKEISNYTDIIYHLIKESQNQQEKNEQDLLALDKWADLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSVINRVRQGYSPLSFRLTPNPREPDRLGGIEEGGGEQDRDRSIRLVSGFLTLAWDDLRSLCLFS"
  },
  {
    "reference": "MTQTSRPAAPIAAVVLAAGKGTRMKSQRHKVLHPIAGRPMIEHLLAALAELQPERTVVIVGDGREQLEKQLAGRAVFAVQEPQLGTAHAVQQAEAALAGFQGDVLVLYGDVPFVSATTMRSMIERLHEEDEPAAVVLGFEPEDTLQYGRVIAQGGRIAWMVEHKDADDGQRQCRLCNSGLLAAPADVLFDLLRKVGNDNAQGEYYLPDAVNVAISEGRTCAVVTTEDPDEVAGINSRAELAEAEARWQRVRRSKAMAEGATLVAPETVFFAWDTQLGRDVTIEPSVVFGPGVTVADGVTIHAFCHIEGAELGEGASVGPFARLRPGTRLAKGVKVGNFVEVKNAELGEGAKANHLSYVGDATVGAAANIGAGTITCNYDGYFKHRTVIGERAFIGSNSALIAPVSIGADAIVAAGSAVSRDVAAGELRMVRAEQLVKPGWADRFHDAMKKKKADPKK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein should be able to manipulate the activity of enzymes in the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway, either by activating or inhibiting their function.2. The protein should be able to bind Mg(2+), UDP-N-acetyl-alpha-D-glucosamine ligand in a variety of conditions3. The protein with the reaction acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate, the Proton acceptor must be optimized to ensure efficient catalysis.4. The designed protein should have glucosamine-1-phosphate N-acetyltransferase activity, magnesium ion binding within cytoplasm to regulate UDP-N-acetylglucosamine biosynthetic process, cell morphogenesis, peptidoglycan biosynthetic process. The designed protein sequence is ",
    "response#1": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVELDDPNGYGRIVRNEKSEVVAIVEQKDATEEQKKIKEINAGIYVFNTESLKQSLAKIGNNNAQGEFYITDVVATLQAEGMNIAAIKPKDANEVLGVNNRIQLSEAEKVMRSRINKKHMLAGVTLVDPRRIDVRGKLACGRDVLIDINAVFEGEVELGDGVQIGANAVIRDTSIGAGTVIHPHCVLEQSRIGANCQIGPYARTRPGVEMADDAKLGNFVEIKKSRIGKGSKINHLSYIGDAIIGANVNVGAGTITCNYDGANKHRTTIGDGAFIGSGVELVAPVEIGAGATVGAGSTITKAVPADELVLERSQQRHIENWVRQKKPGSKSAEAAEKAKSE",
    "response#2": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLTVELPDPTGYGRIVRGGDGVVERIVEEKDASPEERAVREVNTGIMVLPGARLAGWLANLEPRNAQGEYYLTDIFAMAAADGVPVVTAQPPVPVEVEGVNNKLQLAALERAYQRRQAEALMLSGVTLVDPRRIDVRGKLACGRDVLIDINAVFEGEVELGDGVQIGANAVIRDTSIGAGTVIHPHCVLEQSRIGANCQIGPYARTRPGVEMADDAKLGNFVEIKKSRIGKGSKINHLSYIGDAIIGANVNVGAGTITCNYDGANKHRTTIGDGAFIGSGVELVAPVEIGAGATVGAGSTITKAVPADELVLERSQQRTLEGWKRPVKEEK",
    "response#3": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLTAVMEDPYGYGRIVRDQQGNIIRIVEEKDASPEEKALKEIYSGIMAMPAKKLKEWVSKLSNNNAQKEYYLTDIIAMAVRDHIPVQCVQPEEAWEILGVNSKVQLAELERIHQRNIADRLMRSGVTLVDPRRIDVRGKLACGRDVLIDINAVFEGEVELGDGVQIGANAVIRDTSIGAGTVIHPHCVLEQSRIGANCQIGPYARTRPGVEMADDAKLGNFVEIKKSRIGKGSKINHLSYIGDAIIGANVNVGAGTITCNYDGANKHRTTIGDGAFIGSGVELVAPVEIGAGATVGAGSTITKAVPADELVLERSQQRNIRGWKRPEKK"
  },
  {
    "reference": "MKLENMFKKTRAVSGRKGYISLRERRPEVPEGLLRKCNKCGSAIIAEDVKRGYYICPKCGGYFRVHAYRRIEMITDDGTFEEWDKELVGGNPMDYKGYPEKLAAVQEKTKLTEAVVTGKAEINGLETVIGVCDGRFLMSSMGEAVGEKIARAVERATKERLPVILFACSGGARMQEGIISLMQMAKTAAALKRHSDAGLLYISVLTDPTTGGVTASFAMLGDVILAEPKALIGFAGPRVIEQTIGQKLPKGFQRSEFLLEHGFIDRIVAREEQKTVLSEILQMHQKADRPRREQTAPCPEKRMETPAPDKEGGLSAWERVQISRMKDRPVGSDYIQSLFTDFMEFHGDRYFADDKAIIGGIARFHGQPVTVIAQAKGTTTKENIERNFGMPSPDGYRKALRLMKQAEKFGRPVICFVDTPGAFCGLEAEERGQGEAIAKNIYEMSGLKVPVLSIIIGEGGSGGALAMATADEVWMLENSIYSILSPEGFASILWKDSTKAKEAAGVMKLTARDLYKAGVVEKVFSEPSHYTVLNLEEVTGRISEGIEEFLEKYENVPVEELTQRRYERFRRM",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that First, biotin carboxylase catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the carboxyltransferase to acetyl-CoA to form malonyl-CoA.2. The designed protein must have at least one CoA carboxyltransferase C-terminal domain.3. The protein should be optimized for activity in malonyl-CoA from acetyl-CoA: step 1/1 pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. Zn(2+) is required for the proper folding and stability of the protein.5. A protein that contributes to fatty acid biosynthetic process, malonyl-CoA biosynthetic process by functioning in acetyl-CoA carboxylase complex with ATP binding, zinc ion binding. The designed protein sequence is ",
    "response#1": "MQKSGKSEACRNATGKLGGSDAVISGMDFGFIGGSMGSVVGEKIARAADKSVELNAPLIVISQSGGARMMEGAFSLMQMAKTSARLTRLGERGIPFISLMTDPTMGGISASFAMLGDLNISEPKALIGFAGPRVIRDPRRILRSAEELPKTDAWTAVQLARHPERPTTLDYAAHLLDGFRELHGDRIAEDCSAVVGGPGRLNGRPVMLIGHQKGGNDLVERQRRNFGMPSPGGYRKAARLMRMAAKLGLPVVTLIDTPGANPGPEAERGGQAVAIAENLRLMARLPVPIVAVVTGEGGSGGALALAVADRVLICANGIYSVISPEGCAAILWKDSQAAPTAAAALRVDAGELLRLGIVDGVVPEPPGGAHTDHLQAAALLGNAVTAALNRVLRRAGTPAPSRDHTELAAGDPWAAVRSARDLGRPTTLDLISRVFTDFEELRGDRLGGDCAAVVGGPARLDGTPVMVVGQQKGHTAAELAARNYGMPSPQGYRKAARLMRLAAKLGLPVITLVDTPGAYPGLTAEEDGQAFAIADNLRLLSGLAVPVITVITGEGGSGGALALAVADEVLMFSGAVYSVITPEGCAAILWNDPSMASAAARALRVDARELLRLGVVDGVILEPEGGNQADPAMAAERLRGALVPAIRPGELPERDPWETVRRARDLDRPSTLDYARLAFDDFLELHGDRLAKDCPAIVGGPALLGGTPVMLVGTQKGHTVGELTARGFGMASPAGYRKAARLMRLAGKLGLPVVTLIDTPGAHPGLEAEENGQAFAIAENLRLMAGLPVPSVAVVTGEGGSGGALALAVADRVLMHADAVYSVISPEGCAAILWNDAAAAPRAAAALGVDSARLLRLGVVDAVIPEPGPGAPDDALATAGRLRDALLAVLA",
    "response#2": "MWVKCPSCGDLIYTRQFSDNLKVCKCGYHMRLTAREWLGLLDDGSFVEFDAALASVDALGFVSPRHVYEQKLIESRQQTGLNDALITSSGAINGMLLCLAVTEFEFIGGSMGSAYGERLARVIERAADARLPLLTINASGGARQEEGTLALLQMAKVNMALTRLAAAGQPHIALLVDPCYGGVLASYTSVADVIIAEPGARVGFAGRRVIEQTIRQKLPVHFQTAEFLLDHGMIDMVTPRSELRGVLSTLLRLYRDAFAHTSAAQHVPMLEEGTGPVVAGCDKKELTAWERVEISRSKERPTTLSYVQQVFDDFLELHGDRAFRDDGAVIGGIAMFGGQPVTVIGQQKGKNVKENIYRNFGMASPEGYRKALRLMKQAEKFGRPVVTFVDTPGAACGIEAEERGQGEAIARNLLEMSGIQTPMVSILIGEGGSGGALGLAVTDEVWMMENATYSILSPEGFASILWKDGKRAKEASEVMKITAKDLKKLQIIEKVIREPEPANEENLPEIAEEIREDLDAFLRKEPSPVADATLSSEEPNAAWKSVQLARNTHRPTAIYYIRNMIDGFIELHGDRAFGDDGAIVGGIGWLGGQVVTVIAEEKGENLKERIARNFGCPQPEGYRKSLRLMRQAEKFGRPIICLVDTQGAFCGAEAEERGQGNAIAENLQAMAGLRVPVVSVLLGEGGSGGALALAVSNRVAMQEHAVYSVLSPEGFASILWKDRSRAAEAAAVMRMNANQVYDMGIIDAVLAEGDAPAHENPEQAARELRHYLKRSLNEKRGQSFDRIAPATAADPERDAWATVRRARHPGRPTTLDYLETAFDGFVELHGDRLAADCPAIVGGVARLDGWPVMVIGHQKGHTTAELVAHNFGMASPAGHRKALRLMRLAARLGLPVVTLVDTPGADPGVSAEQQGQAAAIAENILALSTLPTPVVAVVTGEGGSGGALALAVADRVLMLENAVYSVISPEGCAAILWPDSSAAPQAARALRLTAADLCRLGVVDEVVPEPPAAAHDEPARAAELLARAVAANLA",
    "response#3": "MQKSGKSEACRNATGKLGGSDAVISGMDFGFIGGSMGSVVGEKIARAADKSVELNAPLIVISQSGGARMMEGAFSLMQMAKTSARLTRLGERGIPFISLMTDPTMGGISASFAMLGDLNISEPKALIGFAGPRVIRDAHIQKEPSPVADATLSSEEPNAAWKSVQLARNTHRPTAIYYIRNMIDGFIELHGDRAFGDDGAIVGGIGWLGGQVVTVIAEEKGENLKERIARNFGCPQPEGYRKSLRLMRQAEKFGRPIICLVDTQGAFCGAEAEERGQGNAIAENLQAMAGLRVPVVSVLLGEGGSGGALALAVSNRVAMQEHAVYSVLSPEGFASILWKDRSRAAEAAAVMRMNANQVYDMGIIDAVLAEGDAPAHENPEQAARELRHYLKRSLNEAGLPYKH"
  },
  {
    "reference": "MIDEKFDAIVVGAGMAGNAATLTLARRGLKVLQLERGEYSGSKNVQGAILYADMLEKLVPDFREDAPLERHLVEQRFWMLDDRSHVGLHYRSEDFNEEKPNRYTIIRAQFDKWFSSKVKEAGATVLCETTVTELVQDAYGKVIGVKTDRSGGQIHADVVVLAEGVNGLLGTKAHLRERPKPEHVALAVKEMHFLPRETIEARFNLQGDEGVVIEAAGTISRGMTGMGFIYANKECISLGIGCLVSDFQKTGETPYGLLERFKSHPSVAPLIEGSEVKEYAAHLIPEGGFKAIPQLFGDGWVVVGDAAQLNNAVHREGSNLAMTSGRIAAEAIFQVKSRREPMSAKNLSLYKTMLEESFVLKDMKKYKDLPALLHINSQNFFLTYPQLVSKAMQNFVRVDGTPKVEKEKRTLRAFVEARSWFGLFGDAFRFARAWR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit oxidoreductase activity.2. For general function, the protein need meet that Probably accepts electrons from FixA/FixB and reduces a quinone. The designed protein sequence is ",
    "response#1": "DAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKNVTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRSYSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILADGVNSILAEKLGMAKRVKPTNVAVR",
    "response#2": "MPDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKNVTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRSYSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILADGVNSILAEKLGMAKRVKPTNVAVL",
    "response#3": "MMDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKNVTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRSYSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILADGVNSILAEKLGMAKRVKPTNVAVL"
  },
  {
    "reference": "MQPAAALESRQPAAETDPEITSIHYRAQEVTPGGLFVAIKGLAADGHDYVETAVARGAAAIVVQAVTGAPSGVVTLVSANTRVALAALADGFYGQPSQAMTVIGITGTNGKTTTSYLIERILQEAGFSTGVIGTINYRYAGEQFPSPVTTPESLDLQRILARMRAAKVTHAVLEVSSHALDLHRVAGCRFDVGVFTNLTQDHLDYHKDMDSYWKSKKRLFTELLPRGPKNDRATAVINRQDPKSRELEEITSLRTIRVGYAADNDLSITDPNCSLSGISGRMVAAGETLEFRSPLVGTHNCENILCAAGVGVALGISLPVICDGIAGLASIPGRLEPVPDDRGRHVYVDYAHTPDALENVIAAVKELAVGRTICVFGCGGDRDRGKRPLMGAIAASGCDLAVVTSDNPRSENPDRIIAQILHGVRSAGGQQLEAADLAGGFERRGYVVEPDRRQAIRLAVAACRPGDTLLIAGKGHENYQILSDRTIAFDDRQVALEMLKEIRCDASN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must have at least one Mur ligase central, Mur ligase N-terminal catalytic domain.2. The protein design should consider the endogenous proteins and enzymes present in the Cell wall biogenesis pathway to avoid interference with native metabolic processes.3. We are looking for a protein with broad substrate specificity for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.4. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.5. The designed protein must be optimized for tetrahydrofolylpolyglutamate synthase activity, magnesium ion binding.6. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding.7. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.. The designed protein sequence is ",
    "response#1": "MKLAELLAGVAPVPAGGTLVTGVASDSRLAGPGDLYLALPGDRTHGARFAAQAVRAGAVAVLTDAAGAALLEGDGSLGPDVPVVVVDRPRALMAGLAAEAWFGHPTARMTVVGVTGTSGKTSVVSFLRQIWAHAGLQAASIGTTGVVAPGRDDYGTLTTPDPVALHLLLKELADAGVTHAAMEASSHGLDQRRLDGVKLSAAGFTNLGRDHMDYHPTVEDYHRAKLRLFDTLLPKGAPAVIFADDAWSEPTVAAANKAGRIVLTVGRNGDFLRLKRLEHERHRQRAEIEFSGALYEVDLPLAGDFQMSNALVAAGLALAAGADPDAVAAGLTAVGGVPGRMQPVPLADHPDARTPQVYVDFAHTPQAVASALAALAPRAHGRPVVCVVGCGGDRDPAKRGSMGAAAVRGADLVVITDDNPRGEEPAAIRAATLAGARDELARTGAATEVVDGGDRRSALALALRRARTSGAVVAVLGKGHESGQIVGDLVLPFDDRVVAREVLAAQGGRAA",
    "response#2": "MGLEVTGVSLDSRSILPGDLYAALPGAVTHGAAFVEGAKAAGAVAVLTDPAGEDRALAAGLPTLVVEKPRNVLGAVAARVFGNPAEQMLGVTGTNGKTTTSYLLDSVLRTIGQQVGLIGTVANYIGSEQLESNRTTPEAVELQRLLAEMSRAGCEAAVMEVSSHALALHRVDDCDFDVAVFTNLTQDHLDFHKTMDAYFAAKKRLFTQLDRNARKVVNLDDAYGKRLAKDCPRVTVGFGIAEADYHATDLVMREDGLRFRLHTARGERELEIPTVGRFNVYNALAALGTVLALGFELGAIAKGVASLKGVPGRFEPVDAGQPYAVIVDYAHTPDSLENVLQTLQDMANGSIYVIAGCGGDRDASKRPEMAKIAVRYSHHAIFTSDNPRNEDPKQILDQMVSGVQQKDDYTVIADRREAIRYAVERANDNDVILIAGKGHETYQILKDGTIHFDEREVVRDVFEHLGVKS",
    "response#3": "MCLEVTGVSLDSRSILPGDLYAALPGAVTHGAAFVEGAKAAGAVAVLTDPAGEDRALAAGLPTLVVEKPRNVLGAVAAALFGITGTNGKTTTSFLVDGGLRAGGWHTGLIGTLGYRIDDRPLDGPRTTVTTPESAELQGLFAALREAGAQAVTMEVSSHALALGRADAVVFDVAVFTNFGRDHLDFHGTLEAYFAAKASLFTAARARRAVINLDDPRGPALVAQARAEGLAVVTTGTADGTAADYRLLELDADGPVTRLTVGTPAGTVRMDLKLPGDHNAHNAVSALAAMTLDSRVAAAGDLFVAVKGHQADGRRYIPQAIAQGVAAVVADADSAAGHGTLTMTHGVPVIAIHNLQQQLSALAGRFHGIGAAEAARGLAGLTPVPGRLERAALNAAGAPAYVDYAHTPDALEAAIAALRPHVAEGGKLIVVFGAGGDRDAGKRPEMGAVAARAADLAIVTDDNPRSEDPAAIRAAILAGAGGNTREIGDRREAIAAAIGEAGARDIVLIAGKGHEQGQEVAGVVRPFDDRVELAQALAARGAGR"
  },
  {
    "reference": "MQRLRRGLFLLLGVGLGILAITGLAAFYVDLAWFAELHALPVLWTRVIARWGLGLGAFLFALAVTGSNICSCWRGATLAGAWAIATGLSVFFAGSLSNHWFTLLLWLNQGPVGESDPIFNRDLGFFLFSLPFWETLQHWCFNLVLLTLIAVVLIYLVELGLSEQRLTLALSLYAQRHLLILGGSLFLIRAWGHWLERYELLYSTRGVVFGAGFADVHATLPATTLMSGVAGLTAVGFWALARQGIRLNLPFLKSWCPSWASSLLAPAILWGAYLGFGLLTTQLYPQLVQTFLVTPNELELERPYIEDNIRFTRAGFGLADVEVQPFPEGGALTWEILQQNQSTLSNVRLWDADPLLATYRQLQEIRPYYQFPYVNVDRYRIGGELRQVMHAARELDFAQVPPAAQTWVNRRFFYTHGYGLTLSPVNVVTPEGLPDFFLSDIPPRVSPRYPEVASVLRVEQPALYYGELTTTDVFVGAEARELDYPAAERYVYSSYRGSGGVPIPHLWQRFLYAWHFGDLRILLSRELSSATRFLYRRQIRERIRRVMPFLLYDRDPYLVIQGGKLYWFFDAYTTSSRYPYSEHLPGFPFNYIRNSVKAVMDAYNGSIDWYIADPRDPLIQAYARIYPTLFKPLEAMPEPLRQHIRYPQDLFRLQAQQFATYHMTDPRLFYNREDQWQIPGQFRNRRRIPMQPQYLILTLPRDPQGVANHTPEFVLLSPYAPVNKQNMVAWIAARCDGENYGKLLVYEFSKQRLIYGPEQVEARVNQDP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a specific function within the plasma membrane.2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "STTPPWWAIPPAWSLGAEIQAYFLLPILLTYKMLGLSVFWISYIIYSLANLNIIHSDYFGYRLIPGVIFMFLSGAYLQKIVSGKASRLEMLSLIIIYIISLFWLVFFIIIKGKYGAYTRETLLGLLVGIPLVYTLLKIRRKFYFNDLFGKLSYGIFLSHFLSFWILEFVNLTQNIISMIFLSLIISASVSYLF",
    "response#2": "STTPPWWAIPPAWSLGAEIQAYFLLPILLTYKMLGLSVFWISYIIYSLANLNIIHSDYFGYRLIPGVIFMFLSGAYLQKIVSGKASRLEMLSLIIIYIISLFWLVFFIIIKGKYGAYTRETLLGLLVGIPLVYTLLKIRRKFYFNDLFGKLSYGIFLSHFLSFWILEFVNLTQNIISMIFLSLIISASVSYLF",
    "response#3": "STTPPWWAIPPAWSLGAEIQAYFLLPILLTYKMLGLSVFWISYIIYSLANLNIIHSDYFGYRLIPGVIFMFLSGAYLQKIVSGKASRLEMLSLIIIYIISLFWLVFFIIIKGKYGAYTRETLLGLLVGIPLVYTLLKIRRKFYFNDLFGKLSYGIFLSHFLSFWILEFVNLTQNIISMIFLSLIISASVSYLL"
  },
  {
    "reference": "MRGDRGRGRGGRFGSRGGLVQGYRPFVPHIPFDFYVCEMAFPRVKPASDETAFSECLLKRNQDLSPTPSEQASILSLVTKINNVIDNLIVAPGNFEVQIEEVRQVGSYKKGTMTAGHNVADLVVILKILPTLEAVAALGNKVVETLRTQDPSEVLSMLTNETGFEISSADATVKILITTVPPNLRKLDPELHLDIKVLQSALAAIRHARWFEENASQSTVKVLIRLLKDIRVRFPGFEPLTPWILDLLGHSAVMNHPSRQPLPLNVAYRRCLQMLAAGLFLPGSVGITDPCESGNFRVHTVMTLEQQDMVCFTAQTLVRVLSHGGYRKILGLEGDASYLTTEMSTWDGVIVTPSEKAYEKPPERKEEEDEALEEGVEAEDESMETQE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should contain one or more DZF domains that are essential for its function.2. The designed protein should have double-stranded RNA binding to facilitate positive regulation of DNA-templated transcription.3. The protein must exhibit the following characteristics: Appears to function predominantly as a heterodimeric complex with ILF3.4. The designed protein's functionality will benefit from Acidic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "RPFVPHIPFDFYLCEMAFPRVKPAPDETSFSEALLKRNQDLAPNSAEQASILSLVTKINNVIDNLIVAPGTFEVQIEEVRQVGSYKKGTMTTGHNVADLVVILKILPTLEAVAALGNKVVESLRAQDPSEVLTMLTNETGFEISSSDATVKILITTVPPNLRKLDPELHLDIKVLQSALAAIRHARWFEENASQSTVKVLIRLLKDLRIRFPGFEPLTPWILDLLGHYAVMNNPTRQPLALNVAYRRCLQILAAGLFLPGSVGITDPCESGNFRVHTVMTLEQQDMVCYTAQTLVRILSHGGFRKILGQEGDASYLASEISTWDGVIVTPSEKAYEKPPEKKEGEEEEEEEE",
    "response#2": "RPFVPHIPFDFYLCEMAFPRVKPAPDETSFSEALLKRNQDLAPNSAEQASILSLVTKINNVIDNLIVAPGTFEVQIEEVRQVGSYKKGTMTTGHNVADLVVILKILPTLEAVAALGNKVVESLRAQDPSEVLTMLTNETGFEISSSDATVKILITTVPPNLRKLDPELHLDIKVLQSALAAIRHARWFEENASQSTVKVLIRLLKDLRIRFPGFEPLTPWILDLLGHYAVMNNPTRQPLALNVAYRRCLQILAAGLFLPGSVGITDPCESGNFRVHTVMTLEQQDMVCYTAQTLVRILSHGGFRKILGQEGDASYLASEISTWDGVIVTPSEKAYEKPPEKKEGEEENVCRTCMDETGTLVDIFANVRDPVLDEPEMSLSHILARCTERPVKRGDLLPQFICVSCVLAVQNAFRFKWQSEQSYQHFFRR",
    "response#3": "RPFVPHIPFDFYLCEMAFPRVKPAPDETSFSEALLKRNQDLAPNSAEQASILSLVTKINNVIDNLIVAPGTFEVQIEEVRQVGSYKKGTMTTGHNVADLVVILKILPTLEAVAALGNKVVESLRAQDPSEVLTMLTNETGFEISSSDATVKILITTVPPNLRKLDPELHLDIKVLQSALAAIRHARWFEENASQSTVKVLIRLLKDLRIRFPGFEPLTPWILDLLGHYAVMNNPTRQPLALNVAYRRCLQILAAGLFLPGSVGITDPCESGNFRVHTVMTLEQQDMVCYTAQTLVRILSHGGFRKILGQEGDASYLASEISTWDGVIVTPSEKAYEKPPEKKEGEEER"
  },
  {
    "reference": "MEVVLATRNPGKVHEILELVRDLPLRVYALDAFPQIADLPEDGLTYTENAISKALTVARLTRRVTMADDSGIEIDAFQGAPGPQSRRFLGDGASDAQRNVRILKMVAQVAPPARTARYRAVVAVATPDGTVRTFEGTCEGELATAPRGAHGFGYDPIFIVPAYGKTMAQLPPAVKNRISHRARAVTAARPYLRQLADALHHHAATPEPSF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.2. The designed protein must be able to perform dITP diphosphatase activity, XTP diphosphatase activity, metal ion binding, ITP diphosphatase activity under specific conditions. The designed protein sequence is ",
    "response#1": "MPIGVPKVPFRNPGEDPTSWVDLYNRLYREKLLFL",
    "response#2": "MPLLDSQGRVINTWADIINRANLGMEVMHERNAHNFPLDLA",
    "response#3": "MSIILP"
  },
  {
    "reference": "MANIIPKIAIIGEGVIGCTSALQISKAIPNAKITVLHDKPFKKSCSAGPAGLFRIDYEENTEYGRASFAWFSHLYRTTKGSETGVKLVSGHIQSDNLESLKQQQRAYGDIVYNFRFLDDRERLDIFPEPSKHCIHYTAYASEGNKYVPYLKNLLLEQKIEFKQQEVTSLDAVADAGYDVIVNCAGLYGGKLAGDDDTCYPIRGVILEVDAPWHKHFNYRDFTTFTIPKEHSVVVGSTKQDNRWDLEITDEDRNDILKRYIALHPGMREPKIIKEWSALRPGRKHVRIEAQKRTSVGNSKDYMVVHHYGHGSNGFTLGWGTAIEATKLVKTALGL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should be able to enhance the efficiency of aspartate catabolic process, D-amino acid catabolic process.2. For general function, the protein need meet that May play a role in the egg-laying events and early development of the worm, in addition to quality control of the germ cells.3. The protein should be able to bind FAD, substrate ligand in a variety of conditions4. The protein should have a low Km value for D-aspartate + H2O + O2 = H2O2 + NH4(+) + oxaloacetate. The designed protein sequence is ",
    "response#1": "RSELPRLMWQTAGICRQAETLQMAIAKLEQWQEQWQQLSSSKLLAHLPEDQKISLSGPGLNEFMQLWAETHNLLDIAQLILTSALFREESRGGHYRLDCPDTKREWQSHTVR",
    "response#2": "RLQISQDPSLNYEYLPTMGLKSFIQASLALLFGKHSQAIVENRVGGVHTVGDSGAFQLGVQFLRAWHKDARIVYIISSQKELHGLVFQDMGFTVYEYSVWDPKKLCMDPDILLNVVEQIPHGCVLVMGNIIDCKLTPSGWAKLMSMIKSKQIFPFFDIPCQGLYTSDLEEDTRILQYFVSQGFEFFCSQSLSKNFGIYDEGVGMLVVVAVNNQQLLCVLSQLEGLAQALWLNPPNTGARVITSILCNPALLGEWKQSLKEVVENIMLTKEKVKEKLQLLGTPGSWGHITEQSGTHGYLGLNSQQVEYLVRKKHIYIPKNGQINFSCINANNINYITG",
    "response#3": "RLQISQDPSLNYEYLPTMGLKSFIQASLALLFGKHSQAIVENRVGGVHTVGDSGAFQLGVQFLRAWHKDARIVYIISSQKELHGLVFQDMGFTVYEYSVWDPKKLCMDPDILLNVVEQIPHGCVLVMGNIIDCKLTPSGWAKLMSMIKSKQIFPFFDIPCQGLYTSDLEEDTRILQYFVSQGFEFFCSQSLSKNFGIYDEGVGMLVVVAVNNQQLLCVLSQLEGLAQALWLNPPNTGARVITSILCNPALLGEWKQSLKEVVENIMLTKEKVKEKLQLLGTPGSWGHITEQSGTHGYLGLNSQQVEYLVRKKHIYIPKNGQINFSCINANNINYITN"
  },
  {
    "reference": "MNEIKSESLLQTRPFKLGIEDIQNLGSSYFIENNEKLKKYNNEISSLKKELDILNEKMGKCTTTTKIVEPAKTPEFTFWYYELKEMKGFQDLVMYEVKKKKKHFKVLSHSCLKYLSNREKMKIKKQEEEEKRLKLYSKNISSYMDVFWKKIEKLVWEEKKRELQQTLNKKKEMRFKKFVKEAIKKIKDARHNNAHELFENKYVSMSSNNNSEIVNNNASSVDNGDKELKEDDLTDQEEEDYLLDEQMSSTDESENKEEEINMLDDEANLPIEELLKRMYGFKSGEDYINFMENEDDANEENVIETSHNDEKSGDNSIGEDDNNNDEKGGDNNIDEDDNNNDEKSGDNSIGEDDNNNDHKSGDNNIDEDDNNNDHKSEDNSIGEDDNNNDEKGGDNNIDENDNNSDHKSEDNNIDENDNNSDHQSDQEQFNHETKDDIIKNSSYEHIDNKNYYNKTGEDYKSDKENYSPTRFHNKLKKEKYDEYDTKLKIEKREEENKNYEKDEHEYESDNYDKEKINKKKELILLKNDIENDSDETSEHIKRDSRSSCQKQNCEKKRRIIKDEYNLRRTKIAKSKPSSDNNNSENDNNNDNNNDNNNDNNDDNNDDNNDDNNDDNNDDNNDDNNDDNNNEHKNDSDDNDDILTCNMDEKHLTKIPPIIKATLRDYQHAGLHWLLYLYKNNINGILADEMGLGKTLQCISLLSYLAYYFNIWGPHLVIVPTSILINWEIELKRFCPCFKILSYYGNQNERYKKRVGWFNKDSFHICISS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Prioritize Basic and acidic residues, Polar residues compositional bias within the protein sequence to achieve desired characteristics.2. The protein contains novel Helicase C-terminal domains that confer a unique function or activity.3. The ATP binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MKSPPWSLQIAWARRVFLGRSFALLAPTGVGKTSFGVSMSSYLAKKGKKTYIIVPTRLLVEQITEKLLLFGVEKKTILAFGDEGSDRREKKLQLKEGFFNVLITTSMFLYKNYDIIPRFFDFIFIDDVDSFLKTAKNIDKVLLLLGFSEESIEKALNLIKLRSKRYKEEHDRSQIEYFLKELKELRQKNRVLVVSSATSTPKSDRVRLFKELLGFEVGAPMFYLRNVFY",
    "response#2": "MKSPPWSLQIAWARRVFLGRSFALLAPTGVGKTSFGVSMSSYLAKKGKKTYIIVPTRLLVEQITEKLLLFGVEKKTILAFGDEGSDRREKKLQLKEGFFNVLITTSMFLYKNYDIIPRFFDFIFIDDVDSFLKTAKNIDKVLLLLGFSEESIEKALNLIKLRSKRYKEEHDRSQIEYFLKELKELRQKNRVLVVSSATSTPKSDRVRLFKELLGFEVGAPMFYLRNVL",
    "response#3": "MMKDILSIIRQGRGKILIYHNRVRMSGVLILQEILQSNGILNEVSSPVGTTRCSICAAIRDEHTHSDHQFIPVRFTILHSEIEPAVRERSLALFNASSNLEGHQLRILIGSKVIVEGLNFQAVRYEMIMSLPLDIPRLIQVFGRVVRKNSHMELPPSERNVTIYLYVSTTPDGGPELAKYAQKLKEYILIQEGDKALRKHL"
  },
  {
    "reference": "MSNLLQAIMNIKSIQERNLGSYKGVQDSKNRMNQMGVTLEVFLKDAFCNTFDIENKDLVYSEYFSYLGNQNNPPDMILKGGDAVEVKKITGIKTSIQLNSSYPKSKLFVSDSRITEACKNCEDWEVKDIIYAIGTIPNRVLKLMFFVYGDCYAASPSIYQRIVEEVKGGLHSTGLEFSETNELGRINRVDPLGITDLRVRGMWIIKHPIKVFKNIIPPESIKNNNFNLIALMKAEKYKQFPKKDRKRIEAEDNIEVTDVKIKDPDNPAKLLDSVLVRYDEI",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have a fast turnover rate for Endonucleolytic cleavage of DNA to give specific double-   stranded fragments with terminal 5'-phosphates..2. The protein must exhibit the following characteristics: A P subtype restriction enzyme that recognizes the double- stranded sequence 5'-GGCC-3' and cleaves after G-1..3. The designed protein must possess type II site-specific deoxyribonuclease activity, DNA binding to support DNA restriction-modification system under certain conditions. The designed protein sequence is ",
    "response#1": "SGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKNKKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQKAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSSTDYFGDPVYVYSLKEGIEDGFLAPYKVS",
    "response#2": "TSEKGDRRIGVMWHTQGSGKSISMLFYAGKLLAQPELKNPTIVVVTDRNDLDGQLFQTFSSGKDLIKQTPQQVEDRDQLRQLLAQNEVGGVFFTTIQKFALNEEESRFPILNERNNIIVISDEAHRSQYGFTQKLHNGKFQTGYARHLRDALPNASFIGFTGTPISLEDKDTQDVFGRYVSL",
    "response#3": "LKRLIQGTSFSMSAPTGMGKTTTIIVFSTFLAKSGKSVLYIVPTKSLLQQICGKMKNIYNDISCGEVDEKRKINIVTTTYINRNFDKIKNYRPNFIAVDDADSIVKSGKTVDRLVTLMGIPGDVYEKAIRLVKLKRLLAIEEKRDEIEKKIAEIERDISKFYGTASQLVVASATLRPKGIKQKALRYITGFDA"
  },
  {
    "reference": "MGGKWSKRSMDKWSAVRKKMRQAEPAADGVGAVSRDLGKPGAITSSNTATNNAACAWLEAQEEEEVGFPVRPQVPLRPMTYKGAVDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRYPLTFGWCFKLVPVKPEKIEEANEGENNSLLQPMSLHGMGDPEKEVLAWKFDSRLAFHHMARELHPEYYKDC",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be able to perform SH3 domain binding under specific conditions. The designed protein sequence is ",
    "response#1": "VTGTNGKTTTSYLIESILVAAGFKVGVIGTINHRFGAFAHPNPMTTPEALDIQRLLRRMVESGVTHVVMEVSSHGLDLNRVDHCQFDCCVFTNLSQEHLDYHKSMDAYFACKKRLFSDLLNKGAKRKNAVAVINHDDPKGELIAHGLTLPIFYSGYSRTDKIWIEDVKTTPEYTSGVIHIPEGMLSFRSSLVGRYNVHNLLAAAGV",
    "response#2": "KFGGSGGSPFSGLSSIAVRSGSYLDXIIIDGVHHGGSGGNLSPTFTFGSGEYISNMTIRSGDYIDNISFETNMGRRFGPYGGSGGSANTLSNVKVIQINGSAGDYLDSLDII",
    "response#3": "GSGGSPFSGLSSIAVRSGSYLDXIIIDGVHHGGSGGNLSPTFTFGSGEYISNMTIRSGDYIDNISFETNMGRRFGPYGGSGGSANTLSNVKVIQINGSAGDYLDSLDIYYEC"
  },
  {
    "reference": "MIWNEAKDYMFIALGLAIYTVAFTVFLMPYQIVAGGVTGLSAIIYYATGFHLENTYIIINGLLLIAALKILGVRFLMKTIFAIFTLYFMLMFAQEIMPKQENGLPFKLMGEGQDFMSMIIGCVMTGIALATVFTHNGSTGGTDIIAASINKFHPNVTLGNVLIAADFCIIGSCMFFPQFGTYMERAHKVMFGFCVMALENYTLDYVMNARRQSVQFMIFSKKWQEIANAIGTTMNHGVTILDGHGWYTGHQMKVLCILAKKNESINMFRLIKMIDPNAFVSQSSVIGVYGEGFDEMHVKIKEDKKMKIVFATNNLNKLTEVRKILGNAFEVLSLNDIKCNDDIPEKGQTLEDNARLKAEYIYNKYHINCFADDTGLEVEALGGAPGVYSARYASMGEEHAQSHDSGANMTRLLRELEQKDNRKARFRTIIALMIDGNITTFEGIVNGEIIRERRGGEGFGYDPIFKPEGYSQTFAEMGTNIKNQISHRARATQKLANYLKKVKI",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP2. The designed protein should have ITP diphosphatase activity, dITP diphosphatase activity, XTP diphosphatase activity to facilitate purine nucleoside triphosphate catabolic process.3. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate binding.4. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.5. The designed protein must have at least one DUF2179 domain.6. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.7. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MNILLATRNKHKTREFTEILKDLPHIKILTLSDFPEVPEIVEDGATFLENAIKKARTIAKLTGLPAIADDSGLVVDALKGRPGVYSARFSGENATDKDNNLKLISELRGITNRKARFISVIAIAFPDGRNLTAYGECEGYIGYEPRGKNGFGYDPIFFVPGVGKTLGQMTNEEKNKISHRGRAIEKLRDFLRKN",
    "response#2": "MNRFFNSITVRGDSDRAIAAHNGDAVAVIPTMGALHRGHLSLIERSRADNDLVIVSIFVNPLQFGAGEDLDRYPRTLDADLAVAEAAGADAVFAPTAADMYPAGFAAWVQPGPLGEVMEGASRPGHFRGVCTVVAKLFNLIQPDRAYFGAKDAQQLAIIRRLVADLNIDVEIVGCPTVREPSGLAMSSRNRYLSESERAQASALYRALQQARQAFAAGERNAGVLIDTVKEELRQSAVAEIEYIELVDPTTLMPLEQIDENGLLAIAARVGTTRLIDNVLLRNRQPID",
    "response#3": "MNILVVAGGHNNERSISLKTAQSVYNALKNNGINAEIFDITQADLASCIGCGKCYTTGFHCAIKDDMQAILEKILEADGFIIASPTYMNNVSGQLKCFMDRTYCMMHRPELAGKTAAGIVTCGGRNGGQEACIMAMQAFWGIHGMNIPAGYYWNLGSDEFKEKEDLADNAGKAKDLADAVKFIARQVK"
  },
  {
    "reference": "MGSKWSKSIVGWPEIRERMRRADPAAERVRRAEPAAEGVGAVSQDLERRGAITSNNTSANNPALAWLEAQEEEEVGFPVRPQVPLRPMTFKGAFDLSHFLKEKGGLEGLIYSKRRQEILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPEEVEKANEGENNCLLHPMSQHGMDDEEGEVLRWKFDSRLALRHIAKEKHPEFYQD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein's functional design necessitates the inclusion of a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Diacidic; necessary for CD4 internalization, Dileucine internalization motif; necessary for CD4 internalization motif.2. The ideal protein would be one that plays a critical role in suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II through SH3 domain binding within extracellular region, membrane, virion component.3. For general function, the protein need meet that Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPAVRERMRRTPPAAEGVGAVSRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGAFDLGFFLKEKGGLDGLIYSKRRQEILDLWVYHTQGFFPDWQNYTPGPGVRYPLTFGWCYKLVPVDPREVEEANEGENNSLLHPMCQHGMEDEEREVLRWKFDSHLAHRHMARERHPEYYKDC",
    "response#2": "MGGKWSKRSGAGWPTVRERMRRAEPAAEGVGPASRDLDKYGALTSSNTAANNADCAWVEAQEEGEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPEKVEEATEGENNSLLHPMCLHGMEDPEKEVLQWKFDSRLAFHHVARELHPEYYKDC",
    "response#3": "MGGKWSKSSIVGWPAVRERIRRTEPAAEGVGAASQDLDKYGALTSSNTATNNAACAWLEAQEEEEEVGFPVRPQVPLRPMTFKGAFDLSFFLKEKGGLDGLIWSKKRQEILDLWVYNTQGFFPDWQNYTPGPGVRFPLTFGWCFKLVPVDPREVEEANKGENNCLLHPMSQHGIEDEDREVLKWKFDSSLARRHLAREKHPEFYKDC"
  },
  {
    "reference": "MASSSMSSQSSGSWTAKQNKAFEQALATYDQDTPNRWQNVAKVVGGKTTEEVKRHYELLVQDINSIENGHVPFPNYRTSGGCTNGRLSQEEKRMRNMRLQ",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Probable transcription factor..2. The designed protein must be able to perform DNA-binding transcription factor activity under specific conditions.3. The designed protein have SANT domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MRKHRGKPANSDLAELVHGSMAGELGFHLRRGQIAAFRQFARTITTTEGVTPGLYGMLQVIANNPGLSQSALAVAMDVDRSSIVKVVNQLEEKGLIVRDTSPTDRRRYCLHMTPPGVQALARIEQAVMRQDQDFSARLSDAERGTLIGLLKRLYG",
    "response#2": "EMRTILMTLLNIETKVMKGDTQQEASKLYPLKGIDKLSDIIETPIQLKGKRTEMRYEAYRMAGNDELCAIAYRRLIAAIDWVESLSPLLGHLTPQDKIALVKSSFAPLMVFNFCARTAEACQDENVLCLCNFAYVPRNISKMYEDTYHLGNGLVERALNELVAVYREYGMREEEIVCVNAMICLNPLAKDVSDSLFEKIVELRNRIADCLFSIVKEVRLSPTPNVCYGHILLSLATVTELANAMSENLQFAQTFSNQGEIPLLTDLFGCG",
    "response#3": "MRKHRGKPANSDLAELVHGSMAGELGFHLRRGQIAAFRQFARTITTTEGVTPGLYGMLQVIANNPGLSQSALAVAMDVDRSSIVKVVNQLEEKGLIVRDTSPTDRRRYCLHMTPPGVQALARIEQAVMRQDQDFSARLSDAERGTLIGLLKRLYG"
  },
  {
    "reference": "MRAMGIQRNWQNLWRWGTMILGMILICCSAAGNLWVTVYYGVPVWRDAETTLFCASDAKAYDREAHNVWATHACVPTDPSPQEVPLGNVTEEFNMWKNNMVEQMHTDIISLWDQSLQPCVKLTPLCVTLNCSNINNIPNVNASSIPKDVKEEIKNCSFNMTTELKDKKQNVYSLFYRLDVVPLETNLKQNSSHSRYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCNDKEFNGTGLCKNVSTVQCTHGIKPVVSTQLLLNGSLAEGKVMVRSENITNNAKNIIVQFNNSVQINCIRPGNNTRKSIHLGPGKVFYATNIIGDIRKAHCNVNRQQWNKALQDVATQLRTHFRNRTIIFNNSLGGDLEITTHSFNCRGEFFYCNTSGLFNGIWNGTQEPNRTESNDTITLQCRIKQIINMWQRVGQAIYAPPIQGEIRCESNITGLILTRDGGIINGTEETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVEREKRAVGFGAFFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQNNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTTVPWNPSWSNKSQSEIWDNMTWMQWDKEINNYTQIIYDLIEESQRQQEKNEQDLLALDKWANLWNWFDISNWLWYIKIFIMIVGGLIGLRIAFAVLSVINRVRQGYSPLSFQTHTPNPRDLDRPGGIEEEGGEQDRDRSIRLVSGFLALAWDDLRSLCLFSYHR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that localizes to virion membrane and can perform structural molecule activity with high specificity and efficiency.2. The protein must exhibit the following characteristics: The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm..3. The designed protein should contain Cytoplasmic topological domains. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLGNVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVELTPLCVTLHCTDLKKNATENGTFTNNNSSGGRMEEGEMKNCSFNITTNIRDEVKKEYALFYKLDIVPIDNGNDNGSTSYRLISCNTTVIKQACPKVSFEPIPIHYCAPAGFAILKCNEKKFNGTGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVVIRSENFTNNAKIIIVQLNESVEINCTRPNNNTRRGIHIGPGRAFYATGDIIGNIRQAHCNISETKWNYTLNLVAKKLREQFGENKTIVFNQPSGGDPEIVMHSFNCGGEFFYCNSTPLFNSSWPFNRTWNDTEGSNNTERNITLQCSIKQIIRMWQKVGKAMYAPPIPGLIRCSSNITGLILTRDGGNNNTNGNETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR",
    "response#2": "EMKNCSFYITTEIRDRRKQEYALFYRSDVVQINGNNISYRLINCNTSAIKQACPKVNFEPIPIHYCAPAGYAILKCNGENFNGTGLCENVSTVQCTHGIRPVVSTQLLLNGSLAEKEIVIRSENITNNAKTIIVQLNETVIINCTRPSNNTRKSIRIGPGQTFYATGDITGDIRQAHCNISATKWINTLQQVANKLRDHFNKTIEFQPSSGGDPEITEHTFNCGGEFFYCNTTNLFNWTFNSISNNINTSTNEITIQCRIKQIINRWQGVGKAMYAPPTAGSINCLSNITGLLLTRDGGNTSEEIFRPGGGDMRDNWRSELYKYKVVRLEPLGLAPTKARRRVVEREKR",
    "response#3": "LWVTVYYGVPVWRDAETTLFCASDAKAYDKEVHNVWATHACVPTDPNPQEIDLKNVTEDFNMWKNNMVEQMQTDIISLWDQSLKPCVQLTPLCVTLDCIDFNSTSSDDSSNNSTSSNNSSSHSNNSHWVEDMKNCSFNMTTELRDKRKTVYSLFYKLDIVPTDSSNSNNSSRHYRLISCNTSTVTQACPKVSFEPIPIHYCAPAGFAILKCKEKNFTGKGQCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVMIRSENITDNSKTIIVQLKEPVNITCLRHNNNTRTSIRIGPGQTFYATGEVIGDPRRAHCNVSRAAWNRTLHGISIQLKKYFPNKTIKFQNSSGGDLEITTHSFNCGGEFFYCNTTDLFNSTWEENSTVTNNTNANENITLPCRIKQIINLWQRVGRAMYAPPIRGIIRCNSTITGLLLTRDGGNGNSSNNETETFRPIGGNMRDNWRSELYKYKVVEIEPIGVAPTRARRRVVQREKR"
  },
  {
    "reference": "MIDASQGPGAGLRPLYLSAQVRELDRRTIAGGIAGFALMQRASAAAWQVLRQRWPEVRSLTVLCGGGNNGGDGHVLAALAAAEGLTVQRVLLRPVDELSGDARLAARMATAAGVGAEPWTPGIRLSGELMVDALLGTGLAGEVRGAFREAIAAVNASGRPVLAIDIPSGLHADTGAVLGDAVLAATTLTFIGDKLGLHIGAAAEHVGELCFRDLGVEALAATDLVPAAHLLDTGLAAAWLPARRRTAHKGDCGHALVLGGAPGFGGAALLAAEACARLGAGKVSLATAPAHVAASLVRRPEVMAHGIRGATDLGQLPAQADVLVVGPGLGQGSWGQGVLQAALETDKPLVVDADGLNLLAAYFAGAEEGMAPRDNWILTPHPGEAARLLGWRVAEIEADRRAAVVALRERFGGVVVLKGAGSLIAGPQGVAVCPFGNPGMASGGMGDVLSGMLGALLAQGVPLEIAARLGVLVHARAADLAVADAGERGLLAGDLASYARILVNPTECDGDV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have metal ion binding to facilitate nicotinamide nucleotide metabolic process.2. The protein should have a high affinity for AMP.3. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration4. The designed protein have YjeF N-terminal domains with specific sequence motifs that are crucial for its activity.5. The desired protein should be able to perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate The designed protein sequence is ",
    "response#1": "MRDIEGRIFAAGMPVAALMEKVAGLITHRILELYPRSQAPCVGILVGPGHNGGDALVVARELHFQGYQIAVCSPFSKLKDLTSQHAQYVKNLKIPVHESLEPLAECDLFIDGLFGFGLGRSLFSPVDQIVNQLNEWSQPVVSIDLPSGLHTDTGEVLGTAVQATQTFCLGLWKLGLLQDQALDYVGAAELIDFDIPLADIVLLTGGNLGMQGAILLAGQACLRAGAGLVRLCQHPDAPPASLVQPELMGCPAGMDEAWASVHVLGPGLGQDEWARARFEHRLAAPEPLVLDADGLNWLAQAPRHRDNWVLTPHPGEAARLLGCTIDEVEADRFAAVSRLQRRYGGVVLLKGAGSLICDGPTIALCDEGNPGMASGGMGDLLSGIIAALLAQGWPAGMAARLGAVIHGEAADLAAAEGERGMLASDLLA",
    "response#2": "LLMMRAAHAILSIAKKKLKGRLWCVAGPGNNGGDAIGVATLALLEKIDVKCIKLSPLVKGDASISYAFSQNLNLQIQDNLPKISSLKQGDIIVDGIFGIGVSRPPEGIMLDAIEWINTAHNNGINVISIDVPSGLNATNGQSFGCTVQADTTIMCLTAKQGCYTGQSPDLTGELLFSDLGIPESAIATLPLQRSPVTHKYKQGHLLLICGSRRYTGGAILTGLGARASGVGMLSIAVPESIKPLLSAQLPEALIVGCPETESGAIAQLQLPEKTDLSSFEAIACGPGLTVDAKPIVQEVLDSDRPLVLDADGLNILVQLDTIPSLQHRQAPTVLTPHAGEFKRLFPDTPDPTKDRITAVLAAAEQSSAVVLLKGARTAIAQADCVWINPESTPALARGGSGDVLTGLLGGLIAQATSKDLPLPEIVATAAWWHSQAGILAADERTEMGVDAFTLTQYLMFVVNRL",
    "response#3": "IMKMDVLPMYRPRTTFAHKGNFGHSLIIGGSYGKIGAVILASQSTLAIGAGLVTANVPKCGYTALQARFPEAMVITDEDEQLITKIDVNIDPTVIGVGIGMGTDPKTISAFSSFLKSNKKPLVLDADALNIISSKSSLLKLLPENTILLPHFGELERLIGKWKDDFERLKKAKAFSKKHKVILVIKGAFTITVFNDRLFVNSSGNPGMATAGSGDVLMGIITGLISQGYEPLVATVFGVYLHGRSGDLAAQDLGYESIIASHMIDYLSEAA"
  },
  {
    "reference": "MNGDLEVDMSRGDFNPSFFLGKLKDDEFESRSLSDDSFDAMSGDEDKQEQRPKKKKRKTKYHRHTSYQIQELESFFKECPHPNEKQRLELGKKLTLESKQIKFWFQNRRTQMKTQLERHENVILKQENEKLRLENSFLKESMRGSLCIDCGGAVIPGEVSFEQHQLRIENAKLKEELDRICALANRFIGGSISLEQPSNGGIGSQHLPIGHCVSGGTSLMFMDLAMEAMDELLKLAELETSLWSSKSEKGSMNHFPGSRETGLVLINSLALVETLMDTNKWAEMFECIVAVASTLEVISNGSDGSRNGSILLMQAEFQVMSPLVPIKQKKFLRYCKQHGDGLWAVVDVSYDINRGNENLKSYGGSKMFPSGCIIQDIGNGCSKVTWIEHSEYEESHTHSLYQPLLSSSVGLGATKWLATLQRQCESFTMLLSSEDHTGLSHAGTKSILKLAQRMKLNFYSGITASCIHKWEKLLAENVGQDTRILTRKSLEPSGIVLSAATSLWLPVTQQRLFEFLCDGKCRNQWDILSNGASMENTLLVPKGQQEGSCVSLLRAAGNDQNESSMLILQETWNDVSGALVVYAPVDIPSMNTVMSGGDSAYVALLPSGFSILPDGSSSSSDQFDTDGGLVNQESKGCLLTVGFQILVNSLPTAKLNVESVETVNNLIACTIHKIRAALRIPA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Seems to promote cell differentiation.; Probable transcription factor that binds to the DNA sequence 5'-GCATTAAATGC-3'.2. The Homeobox domain within the designed protein should exhibit high affinity and specificity towards its target DNA sequence.3. The designed protein should be involved in cell differentiation by localizing to nucleus and exhibiting sequence-specific DNA binding, DNA-binding transcription factor activity.4. The designed protein should contain one or more START domains that are essential for its function. The designed protein sequence is ",
    "response#1": "ACATTAAATGC",
    "response#2": "GCATTAAATGC",
    "response#3": "RATTAAATGC"
  },
  {
    "reference": "MAKEEKRVVAQNRKARHDYFIEQTIEAGIVLSGTEVKSIRAGKVSLKDSYANVKNGEVFIYGMHISPYEQGNIFNKDPLRDRKLLLHRAEINKLIGYIQQKGMTLVPLEVYFKNGKVKIELGIAKGKKLYDKREDIAKRDALREIDRRLKENFR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have RNA binding within cytoplasm to regulate trans-translation.2. The protein must exhibit the following characteristics: Required for rescue of stalled ribosomes mediated by trans- translation; tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame; The ribosome is freed to recommence translation, which seems to be the essential function of trans- translation.; During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA; The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation; Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes; tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. The designed protein sequence is ",
    "response#1": "MLKKRIIPCLDIENGRVVKGINFLNLRDAGDPVELASRYYREGADELVFLDITATHEDRRTIVDLVKKTAKNIFVPLCVGGGLRSIKDIKELLRAGADKVSINSAAIKNPKLISLGAEKFGSQCIVVAIDAKQIKNSWEIFTHGGRKNTTLDAIEWAKKVEELGAGEILLTSMDRDGTKTGYDIPLTKAISKAVSIPVIASGGAGKLEHFYSAFTEGKADAVLAASIFHFKEISIKEVKRYLVKERIPVRI",
    "response#2": "MHLPTTAPGVLTAQADKPLVAGLVLTGTEVKSGDKSVPLKQVWFAGHKDSVATPANLAKFYKDVSGKAGYGDWTGVHLTGLSLQGWEVKSGEAWPNAKLVVDQLHLLKLAGKAVDEVRRRVCQEAQDKLALKGRDKLTEKQKQTLTQKLEVLLEKHADITDLKDAKETIEWFYEAESEIAAGYGFKQALALGRKVMSGHIKGLSRFRRTGIEPTSSTVEGHVNHILSDRMASRNHYGKSPEGQARLARLAVIRNGGDYQQLIAA",
    "response#3": "MEKQSTSTTGTPASSTGKPPVVFVTGNANKLKEVRGILSQTTGSKSIEVDSTNLDIEEVQGSSQEIARGKCLEASNRVEGPVLVEDTSLCFNALGGLPGPYIKWFLDSCGHEGLNNLLAAYDDKSAYALCTFAFSSGPDSEVKLFEGRTSGRIVPPRGPRDFGWDAVFEVDGTGKTYAEMPKEVKNTISHRYRALDKLKEYLQK"
  },
  {
    "reference": "MLQLGSRCSERLWVSVKLRGPQRRSKECPTRTSTTPRRLSRAEPASFPGSRARRRDMGARPLAAGGGSAQGPPTGRAPPPPPPLEPNMAMHNKTAPPQIPDTRRELAELVKRKQELAETLANLERQIYAFEGSYLEDTQMYGNIIRGWDRYLTNQKNSNSKNDRRNRKFKEAERLFSKSSVTSAAAVSALAGVQDQLIEKREPGSGTESDTSPDFHNQENEPAQEDPEDLDGSVQGVKPQKAASSTSSGSHHSSHKKRKNKNRHSPSGMFDYDFEIDLKLNKKPRADY",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in nucleoplasm, histone acetyltransferase complex, kinetochore, MOZ/MORF histone acetyltransferase complex, nucleolus that is critical for regulation of cell cycle, regulation of cell growth, regulation of DNA biosynthetic process.3. The protein must exhibit the following characteristics: Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A; This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription; Component of HBO1 complexes, which specifically mediate acetylation of histone H3 at 'Lys-14' (H3K14ac), and have reduced activity toward histone H4. The designed protein sequence is ",
    "response#1": "EGSNMIKTIAFGRYELDTWYHSPYPEEYARLGRLYMCEFCLKYMKSQTILRRHMAKCVWKHPPGDEIYRKGSISVFEVDGKKNKIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVMTEADNTGCHLIGYFSKEKNSFLNYNVSCILTMPQYMRQGYGKMLIDFSYLLSKVEEKVGSPERPLSDLGLISYRSYWKEVLLRYLHNFQGKEISIKEISQETAVNPVDIVSTLQALQMLKYWKGKHLVLKRQDLIDEWIAKEAKRSNSNKTMDPSCLKWTCS",
    "response#2": "EGSNMIKTIAFGRYELDTWYHSPYPEEYARLGRLYMCEFCLKYMKSQTILRRHMAKCVWKHPPGDEIYRKGSISVFEVDGKKNKIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVMTEADNTGCHLIGYFSKEKNSFLNYNVSCILTMPQYMRQGYGKMLIDFSYLLSKVEEKVGSPERPLSDLGLISYRSYWKEVLLRYLHNFQGKEISIKEISQETAVNPVDIVSTLQALQMLKYWKGKHLVLKRQDLIDEWIAKEAKRSNSNKTMDPSCLKWTL",
    "response#3": "EGSNMIKTIAFGRYELDTWYHSPYPEEYARLGRLYMCEFCLKYMKSQTILRRHMAKCVWKHPPGDEIYRKGSISVFEVDGKKNKIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVMTEADNTGCHLIGYFSKEKNSFLNYNVSCILTMPQYMRQGYGKMLIDFSYLLSKVEEKVGSPERPLSDLGLISYRSYWKEVLLRYLHNFQGKEISIKEISQETAVNPVDIVSTLQALQMLKYWKGKHLVLKRQDLIDEWIAKEAKRSNSNKTMDPSCLKWTL"
  },
  {
    "reference": "MSMKWTSALLLIQLSCYLSSGNCGKVLVWPTEFSHWMNIKTILDELVQRGHEVTVLAYSPSILPGPNNPSALKFEICPTSLTETEFEDSVTQLVKRWSDIPKDTFWPHFLQVQEMMWTYGDMIRKFCKDVVSNKKLMKKLQESRFDVVLADAISPCGELLAELLKIPFVYSLRFSPGYAIEKHGGGFLFPPSYVPVVMSEFSDQMTFMERVKNMIYMVYFDFWFQAWDTKKWDQFYSEVLGRPTTLFETMAKAEIWLIRNYWDFQFPHPLLPHVELVGGLHCKPAKPLPKEMEGFVQSSGDNGVVVFSLGSMVSNMSEERANVIASALAKIPQKVLWRFDGNKPDTLGLNTQLYKWLPQNDLLGHPKTRAFITHGGANAIYEAIYHGIPMVGVPLFADQLDNIAHMKAKGARVSLDFNTMSSTDLLHALKTVINDPFYKENAMKLSSIHHDQPVKPLDRAVFWIEFVMRHKGAKHLRVAAYDLTWFQYHSLDVIGFLLACVATVIFIITKCLFCVLKFVRTGKKGKRD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds The designed protein sequence is ",
    "response#1": "VMDFRTQLIRLCYSSDHEKLKPQYLEELPGQLKQFSVFLGKFSWFAGEKLTFVDFLTYDILDQNRIFEPKCLDEFPNLKAFMCRFEALN",
    "response#2": "WIPQNDLLGHPKTKAFVTHGGANGIYEAIHHGIPMVGLPLFGEG",
    "response#3": "VMDFRTQLIRLCYSSDHEKLKPQYLEELPGQLKQFSVFLGKFSWFAGEKLTFVDFLTYDILDQNRIFEPKCLDEFPNLKAFMCRFEALGA"
  },
  {
    "reference": "MGIKHLFQVIQENAPDAIKAGDIKNHFGRKVAIDASMSIYSFLIAVRSEGQQLMSESGETTSHLMGMFYRTLRMVDNGIKPLYVFDGAPPKLKSGELAKRTARKAEATEAHEEAKETGTAEDVEKFSRRTVRVTREHNAECKKLLKLMGIPYIDAPTEAEAQCAVLARAGKVYAAASEDMDTLCFEAPILLRHLTFSEQRKEPIQEIHLNRALEGLGMDRKQFIDLCILLGCDYLEPIPKVGPNTALKLIREHGSLEKVVEAIENDPKKKYVIPEYWPYQDARELFLHPDVREADHPECDFKWEAPDVEALVEFLVKDKGFNEDRVRNGATRLQKNLKTAQQSRLEGFFKPVARTDEEKASLKRKHDEKLQEQKKRKKEEAKAKKEAKAKPRGAA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that can perform magnesium ion binding, DNA binding, 5'-flap endonuclease activity, 5'-3' exonuclease activity in mitochondrion, nucleoplasm, nucleolus, cytoplasm to support base-excision repair, DNA replication, removal of RNA primer.2. The protein should exhibit Basic and acidic residues compositional bias.3. For general function, the protein need meet that Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.; Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity; Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions; It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation; During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment; Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site- terminated flap; Structure-specific nuclease with 5'-flap endonuclease and 5'- 3' exonuclease activities involved in DNA replication and repair4. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+) binding. The designed protein sequence is ",
    "response#1": "MGIKGLTGLLSQHAPKAIQEHEIKTLFGRKVAIDASMSIYQFLIAVRQKDGELLTNDAGETTSHLMGLFYRTLRIVENGIKPAYIFDGKPPELKKGVLSKRLERREEGLPYVEAPCEAEAQCAELAKAGKVYAAASEDMDTLCYRTPFLLRHLTFSEAKKEPIHEINTEILLQGLELSIEQFIDLGIMLGCAGLPYVEAPCEAEAQCAELAKAGKVYAAASEDMDTLCYRTPFLLRHLTFSEAKKEPIHEINTEILLQGLELSIEQFIDLGIMLGCLPTYREQLIDLAILLGTDYNPEGVPGVGPQKALKLIWEYGSLENIITYMGIPWVQAPSEGEAQAAHMAKRGHCWAVGSQDYDSLLFGSPRLVRNLAVSPKRKVGEEVVELSPELIELDAVLKSLKREKLIELAILVGTDYNPGGIKGIGPKKALTIVKRYGSLENIITYMGVPWVQAPSEGEAQAAHMAKRGHCWAVGSQDYDSLLFGSPRLVRNLAVSPRRKIGEEVIELSPEIIELDAVLKSLRYGSLEKIIKTAKELGIPYIEAYGEGEAQAAYLVENGDAWAVASQDYDCLLFGAKRVVRNLAINSNLGDLEYYNLKRVLDELDINREQLIDMGILIGTYGSLEKIIELAKELGVKENKVIAIDASVMLYQFITTITSGDGTALANSSGEVTSHLVGLLAKVIRLAEAGIKPIFVFDGKPPEDKQGELEKRRQAREAAELEQQKAEEEGNLERAKQLSRRTVKVTQQHCKQAERLLDTLGIPYVVAAGEAEAQCVAMAKERVCEGVASSDLDVLAFGSPCLIRNLAQGGDREIVEINLNTVLKELGFSYDEFLDLCILCGCDYANSLEGIGPKTAYKLIVKHRSIEEVLAADQGKLAEKALNWPYVRARELFKNPAVIDPKEIKKTLKWKPVNRGEAMQFLVEEMEFDRETTEKKL",
    "response#2": "MGITKLAHLIHFDAPASMRSKEIGDYSGKIIALDTSIVVNQFRSALPGHLKLSPLAGLFYRTLAFLEHDIKPVFVLDGKPPHQKRAVLEKRAQSTGWLNHVDGIPVVEAPCEAEAQCANLVKAGKVYGTATEDMDALTFGSCVLLRHLLAPEAKKIPIKEFHLARVLEEMQLTKDQFIDLCILLGCIGLPYVEAPCEAEAQCAELAKAGKVYAAASEDMDTLCYRTPFLLRHLTFSEAKKEPIHEINTEILLQGLELSIEQFIDLGIMLGCIPHLGGLGVDAWQQLVDACSRTLERIVKQNPFKNVGEIHKEWTAAGVSASRTTTHRRMQDMGFSCRIPCVKPLLNNRQRQKRHGRTLERIVKQNPFKNVGEIHKEWTAAGVSASRTTTHRRMQDMGFSCRIPCVKPLLNNRQRQKRHGGSVVAVDAHNWLYRYLTTTVKFTSESKYTTSNGEEVANLIGVVQGLPKFFEHDMTPVFVFDGAVTDLKDDEVEKRREQRQKYESELEAAREAGDSTRVAKLDSRTQRLTDTIVDTTRDLLELLDVPIVDAPAEGEGQASVMARRGDVDYVGTEDYDALLFGAPMTLRQITSKGDPELMDFAATLEHHDLTWEQLVDAAILMGTDFNEGISGIGPKTAVKDLHEHGDLYTVLAARGEHIDHADRIRDLFLDPAATDDYEIKRVRE",
    "response#3": "MGITKLAHLIHFDAPASMRSKEIGDYSGKIIALDTSIVVNQFRSALPGHLKLSPLAGLFYRTLAFLEHDIKPVFVLDGKPPHQKRAVLEKRAQSTGWGHNQFYEIKPCYVFDGKPPVLQLGLMGIPVVEAPCEAEAQCANLVKAGKVYGTATEDMDALTFGSCVLLRHLLAPEAKKIPIKEFHLARVLEEMQRQHLMGVPCIKAPGEAEALCAHLAKIGTVNAVASEDMDTLAFGGTVLLRQLNAKRDSEITEYSLPKLLEALQMSQFIDLCILLGCDYLEPIKGVGPKSALKLIREYGSLERTLERIVKQNPFKNVGEIHKEWTAAGVSASRTTTHRRMQDMGFSCRIPCVKPLLNNRQRQKRLGIPYVNAPGEAEAQCAELAKKGKVYAAASEDMDTLCYRTPYLLRHLTFSEARKEPIHEINTEIVLQGLELTIDQFIDLGIMLGCEYSRHIDQFIDLGIMLGCDYCDSIKGVGPVTALKLMKEYGSLERTLERIVKQNPFKNVGEIHKEWTAAGVSASRTTTHRRMQDMGFSCRIPCVKPLLNNRQRQKRLSVPIIEAPAEGEAQAAEMAIRGDVDYVGSEDYDTLLFGAPYTVRQLTSKGDPELMDLQTTLKNQNLTREQLVDVAILCGTYRSPTRHKIGGIPWVQAPSEGEAQAAHMAKRGHCWAVGSQDYDSLLFGSPRLVRNLAVSPKRKVGEEVVELSPELIELDAVLKSLKLKGREQLIDLAILLGT"
  },
  {
    "reference": "MTKRIAITAGEPASIGPDLVITLAQQAWPAELVVCANPELLLARAAKLGLPLRLIPYHSENKPQPQAAGTLTIAPFELAAEVECGVLNELNSAYVVDTLRFAGEKNMSREFDAVVTGPVHKGIINQAGIAFSGHTEYFAVQANCQDVVMMLAAPGLQVALMTTHIPLAYVAKAITRERLHHIIHILHKELKSKFGLGSPKIYVCGLNPHAGEDGHIGREELDVMIPALNELRAQGIQLVGPLPADTLFQPKYLQDADVILAMYHDQGLPVLKSLGFGKSVNITLGLPYIRTSVDHGTALELAGTGLADSGSFTCALNKAIELASKVSN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Catalyzes the NAD(P)-dependent oxidation of 4-(phosphooxy)-L- threonine (HTP) into 2-amino-3-oxo-4-(phosphooxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).2. The protein should have a fast turnover rate for 4-(phosphooxy)-L-threonine + NAD(+) = 3-amino-2-oxopropyl   phosphate + CO2 + NADH.3. Ideal protein should have a binding affinity for key enzymes in the pyridoxine 5'-phosphate biosynthesis pathway to enhance the overall efficiency of the pathway.4. The designed protein must be able to perform magnesium ion binding under specific conditions.5. The designed protein should have a high specificity for substrate binding, with minimal off-target effects.6. The protein must bind to Zn(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "APGKDFTRELVQDADALIIRTRTHCNRELLEGSKVKFIATATIGFDHIDTEYCKQAGIEWANAPGCNSASVAQYIQSSLLIWKSLRNKKPDELTIGIIGVGNVGSKVAKVAQDFGMRVLLNDLPREEKEGNITFTSLEKIAEECDIITFHVPLYKEGKYKTYHLADGNFFRSLQRKPVVINTSRGEVIETNALLEAINNGIISDAVIDVWEHEPEINRELLEKVLIGTPHIAGYSADGKANATRMSLDSICRFFSR",
    "response#2": "MNAPGKDFTRELVQDADALIIRTRTHCNRELLEGSKVKFIATATIGFDHIDTEYCKQAGIEWANAPGCNSASVAQYIQSSLLIWKSLRNKKPDELTIGIIGVGNVGSKVAKVAQDFGMRVLLNDLPREEKEGNITFTSLEKIAEECDIITFHVPLYKEGKYKTYHLADGNFFRSLQRKPVVINTSRGEVIETNALLEAINNGIISDAVIDVWEHEPEINRELLEKVLIGTPHIAGYSADGKANATRMSLDSICRFFD",
    "response#3": "APGKDFTRELVQDADALIIRTRTHCNRELLEGSKVKFIATATIGFDHIDTEYCKQAGIEWANAPGCNSASVAQYIQSSLLIWKSLRNKKPDELTIGIIGVGNVGSKVAKVAQDFGMRVLLNDLPREEKEGNITFTSLEKIAEECDIITFHVPLYKEGKYKTYHLADGNFFRSLQRKPVVINTSRGEVIETNALLEAINNGIISDAVIDVWEHEPEINRELLEKVLIGTPHIAGYSADGKANATRMSLDSICRFFR"
  },
  {
    "reference": "MTLLYDSRQIRKIEQWVIAAGTEEYQLMERAGRFVFSILQQRWPDAQKIIFCTGKGNNAGDAYVAARLAHEHGLPVVIYAVTPCDQLKGSSAKAALACRKAGVSIIDYHPELSFDSDVLVDALFGIGLNGVLEEPYVSLINLINDQNIDILAIDVPSGLNADTGCIEGAAIDADVTVTFIGYKPGLVTLKGPSRCGEVILNTLDVPMEILNKIEPIAEFTEWERIQKVFPRRMRDAHKGDYGHVLVIGGDYGMGGAVRMAAEGALRVGAGLVSVATRPEHVPVVSSSRPEIMCHQVGEPCELIPLLEKVSVIVIGPGLGRTAWAEGLMQLVLEAPHPKLLDADALNLLSQRPSYSDRWVLTPHPGESARLLHCSCQELQLDRFDSLRELQKKYGGVVVLKGAGTLVKGKADRIKVCRAGNPGMATGGMGDILSGIIGGLMAQGLDLLTAAEIGVYVHARAGDLAVAEGGERGLLATDVLAYLRPLVNPN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein with NADPHX epimerase activity functions is required to enable nicotinamide nucleotide metabolic process.2. The protein should be able to bind AMP, K(+) ligand in a variety of conditions3. The designed protein have YjeF N-terminal domains with specific sequence motifs that are crucial for its activity.4. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.5. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.. The designed protein sequence is ",
    "response#1": "MLVAGAPETMGAALLTSRACLYAGAGLTSACIPATGLTALNASLPEVMAVVRSEQGLPTSVDWEKYQAIGIGPGLGTGPGSLSLLRVVLKNFHKPIVLDADALNLIAHNYELIQQVPEHSVLTPHMKEFDRLFGDHQDWWSRIETGIDRATTLGCTIVLKNRYTIIFTPQGMCLFNPTGTPAMATGGSGDALTGIITSFLAQGYEPHEAAFIGTYVHGAAGERVPRSDKYSRGVVGLDTGSLAYPGAALLGVDGALHTGAGMVRYAGPARESVLAAFPSVVAPLDPLAPGRVQAWVCGSGWPSADAARLEHRLRDRVPTVIDAGALTGLPATLPPGCLLTPHAGELARLLSVDRHDVEADPLAHARTAVRRTGATVLLKGATQYCVRPDGAAMIAEPGPAWSATAGSGDPV",
    "response#2": "MKQYDEYLLNHGYQINELVDKASDCLIKHMHGQRYCLLCGPGNNGADGLSLAIKLFLQGKQVTVYIFEDQNHLSQANRYYLDQCYALNLEVILLNEDILDEVILHMKDCDIIVDAMFGNGLNSSPRGLYQMVIEQINQLYDQEIIAVDIPTGLNCDNGKPYQSVVCATQTITLTALKNGFLNPDSTSFTGQVIVEELDVEDVSEEENDIRERLIHRAAFSHKGTFGHAMLVAGAPETMGAALLTSRACLYAGAGLTSACIPATGLTALNASLPEVMAVVRSEQGLPTSVDWEKYQAIGIGPGLGTGPGSLSLLRVVLKNFHKPIVLDADALNLIAHNYELIQQVPEHSVLTPHMKEFDRLFGDHQDWWSRIETGIDRATTLGCTIVLKNRYTIIFTPQGMCLFNPTGTPAMATGGSGDALTGIITSFLAQGYEPHEAAFIGTYVHGAAGERVRGYVATPSQLIDELPLLMKELAKA",
    "response#3": "MLVAGAPETMGAALLTSRACLYAGAGLTSACIPATGLTALNASLPEVMAVVRSEQGLPTSVDWEKYQAIGIGPGLGTGPGSLSLLRVVLKNFHKPIVLDADALNLIAHNYELIQQVPEHSVLTPHMKEFDRLFGDHQDWWSRIETGIDRATTLGCTIVLKNRYTIIFTPQGMCLFNPTGTPAMATGGSGDALTGIITSFLAQGYEPHEAAFIGTYVHGAAGERVPRSDKYSRGVVGLDTGSLAYPGAALLGVDGALHTGAGMVRYAGPARESVLAAFPSVVAPLDPLAPGRVQAWVCGSGWPSADAARLEHRLRDRVPTVIDAGALTGLPATLPPGCLLTPHAGELARLLSVDRHDVEADPLAHARTAVRRTGATVLLKGATQYCVRPDGAAMIAEPGPAWSATAGSGDS"
  },
  {
    "reference": "MSDFIQSYNNDPFLGNLSTPVSTSTFTKGLLNNLPAYRRGLSPLLRGLEIGMAHGYFLVGPFDKLGPLRNTDVALLSGFLSAVGLIIILTVCLSMYGNVSFDKDDAKDLLQTTEGWGQFTAGFLVGAVGGSGFAYLLLANIPVLQNLGLS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be expressed specifically in the photosystem I reaction center The designed protein sequence is ",
    "response#1": "VFPAGRDPQWGNLETPVNASPLVKWFINNLPAYRPGLTPFRRGLEVGMAHGYFLFGPFAKLGPLRDAANANLAGLLGAIGLVVLFTLSLSLYANSNPPKALASVTVPNPPDAFQSKEGWNNFASAFLIGGIGGAVVAYFLTSNFI",
    "response#2": "VFPAGRDPQWGNLETPVNASPLVKWFINNLPAYRPGLTPFRRGLEVGMAHGYFLFGPFAKLGPLRDAANANLAGLLGAIGLVVLFTLSLSLYANSNPPKALASVTVPNPPDAFQSKEGWNNFASAFLIGGIGGAVVAYFLTSNFL",
    "response#3": "VFPAGRDPQWGNLETPVNASPLVKWFINNLPAYRPGLTPFRRGLEVGMAHGYFLFGPFAKLGPLRDAANANLAGLLGAIGLVVLFTLSLSLYANSNPPKALASVTVPNPPDAFQSKEGWNNFASAFLIGGIGGAVVAYFLTSNFI"
  },
  {
    "reference": "MLKEFKEFALKGNVMDLAVGVVIGGAFGKIVTSLVNDIITPLIGLLLGKVDFSGLFINLSGVPYKTIAEAKTAHAATLNYGLFLNSVIDFVIIAFSIFIVIKQLNRFKRKQEVEQAPVTTKECPHCISAIPVKATRCPNCTSMLETKGTALAHE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein can be targeted to plasma membrane and perform mechanosensitive ion channel activity. The designed protein sequence is ",
    "response#1": "RTLFINGISKYAESEKIKKHFEEAYPNCTVLEARPCYNVARLMFLDAERKKAERGKLYFTNLQSKENVPTMINPKPCGHLCCCVVRGCEQVEAIEYYTKLEQKLKEDYKREKEKVNEKPLGMAFVTFHNETITAIILKDFNVCKCQGCTCRGEPRPSSCSESLHISNWTVSYAPDPQNIYWEC",
    "response#2": "FMLCSLIIKEKLFLGEATMATATGLIFGPYVAKLFVPTSWGNTDYITEELARVLLVVEVFAAGAELPRAYMLRHWRSMFVMLLPVMIFGWLVSTGFMYALIPRLSFLESLAIAACITATDPVLASSIVGKGKFARRVPGHLRNMLLAESGCNDGMAIPFLYLAIYLIIEKPARHAGRDWVCIIILYECTFGCVLGAIIGVIARKMIKFSERRGLMDRESFLVFYFVLALFCGGIGTIIGVDDLLVSFCAGAAFSWDSWFSKKTEESHVSNVIDLLLNLSFFVYVGAIMPWPQFHMPHMDLSVWRLVVLAICILIARRIPAVLLFKSFVPDIINWREALFAGHFGPIGVGALYTCLVARAELEVHSTVPEPNDAIENPEIPNWYCIQVMWPVVCFLVLSSIIVHGSSIAFFVSTTIGVTYNTPVEKIRKAEEIIKNILLEHPNVEDEPITVYFKEFGDWSLNIQVVYYIKNSRYNGYQKYISTINEVNLKIKEEFDRKGIEE",
    "response#3": "FMLCSLIIKEKLFLGEATMATATGLIFGPYVAKLFVPTSWGNTDYITEELARVLLVVEVFAAGAELPRAYMLRHWRSMFVMLLPVMIFGWLVSTGFMYALIPRLSFLESLAIAACITATDPVLASSIVGKGKFARRVPGHLRNMLLAESGCNDGMAIPFLYLAIYLIIEKPARHAGRDWVCIIILYECTFGCVLGAIIGVIARKMIKFSERRGLMDRESFLVFYFVLALFCGGIGTIIGVDDLLVSFCAGAAFSWDSWFSKKTEESHVSNVIDLLLNLSFFVYVGAIMPWPQFHMPHMDLSVWRLVVLAICILIARRIPAVLLFKSFVPDIINWREALFAGHFGPIGVGALYTCLVARAELEVHSTVPEPNDAIENPEIPNWYCIQVMWPVVCFLVLSSIIVHGSSIAFFVSTTIGVTYNTPVEKIRKAEEIIKNILLEHPNVEDEPITVYFKEFGDWSLNIQVVYYIKNSRYNGYQKYISTINEVNLKIKEEFDRKGIEES"
  },
  {
    "reference": "MKIMEPEIKIVNVVVSTKIGDNIDLEEVAMILENAEYEPEQFPGLVCRLSVPKVALLIFRSGKVNCTGAKSKEEAEIAIKKIIKELKDAGIDVIENPEIKIQNMVATADLGIEPNLDDIALMVEGTEYEPEQFPGLVYRLDDPKVVVLIFGSGKVVITGLKSEEDAKRALKKILDTIKEVQEL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The target protein must exhibit strand-helix-turn-strand-turn-strand-strand-helix-strand-helix-strand-turn-strand-turn-strand as its primary conformation.2. The designed protein must be optimized for general transcription initiation factor activity, DNA binding, DNA-binding transcription factor activity.3. For general function, the protein need meet that Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation . The designed protein sequence is ",
    "response#1": "DPETVTSTTNNTWKRCGVTLLPILTTHVKICPVSKQRVIDIKRDDLNDYGWFTRGLLERFNEISVYCGTR",
    "response#2": "EPKLKSVLTMNPRMRKFQVDMTFDVDTANNYLIISEDLRSFRSGDLSQNRKEQAERFDTALCVLGTPRFTSGRHYWEVDVGTSQVWDVGVCKESVNRQGKIVLSSEHGFLTVGCREGKVFAASTVPMTPLWVSPQLHRVGIFLDVGMRSIAFYNVSDGCHIYTFIEIPVCEPWRPFFAHKRGSQDDQSILSICSVINPS",
    "response#3": "DPETVTSTTNNTWKRCGVTLLPILTTHVKICPVSKQRVIDIKRDDLNDYGWFTRGLLERFNEISVYCGTR"
  },
  {
    "reference": "LCQVTETGTDSLPAWLHWDASSRVLRGLPLEEDKGVHYISVTISNHSESSSSSEVFSIEVHPEDHVDADAAQVASNQVSTEDDVQPFACSNEAPITLLTVILDADLTKMSSEQRVELLDDMRIFSGVGLQHMKILPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLDQSTVPNISSVQGPAKEGTMSAKLGYPVVGWHIANKKPHIPKRVRRQLNNTPTPVLTAVPPTTIVEPPVRIIPTLSSPSIAAPTESSAPPVRGPVPLPGKPTIRMRDPVNHTPTLAPPLPTRVVETTSTIAIQPTMTRPPYVEPTATVTAPPKRPTKKPKRPKTTPVTREPKTSTAKPARRTTPSPAVNIDPSNEKPVLRNPIDQVNALVGTYFEVKIPSDTFFDKEDGTTDKLRLTLRQNHNEVVGEASWIQFNTTSQLLFGLPDVQHVGKHEYFMQATDKGGLNAIDAFEVRVNRWPINDKSPVIFKARFEGDPHSITNDIHKKILLVKKLAYALGDRNTSAVSLRNISKGSIVVEWTNTSLPQHPCPKEQLTVMSRTLASADGRPSQTFRHSLEPEFRPLDVTAKGRASCRTYSFIPPGDIDIPEPAAVTPALGTGRQSTDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTIEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKPPLPPPEYPNMATPETTPLNQELLGEYTALQDEDPNAPPYQPPPPFTTPMEGKGSRPKNMTPYRSPPPYVPP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should be able to enhance the efficiency of T-tubule organization, skeletal muscle fiber development.2. The protein must exhibit the following characteristics: Transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. The designed protein sequence is ",
    "response#1": "TPMHKEARIQLPDMQMIDARVDFSVNSFKATKKLCRDLGIRYSEELSLKRYIPPEDLRRGTSDADNMNGPLSMRPGEESVGPMTLRKAAPIFASQSNLDMRRRGQSPALSQSGHIFNAHEMGTLPRHGTLPRGVSPSPGAYNDTMRRTPIMPSISFSEGLENEQFDDALIHSPRLAPSRDTPVFRPQNYVEKAAINRGWLDSSRSLMEQGIFEGDIILLRFKFMNFFDLNPKYDPVRINQLYEQAKWSILLDEFDHTEEEATLFAALQLQATLQRDSPEPEENNKDDVDILLDELEQNLDAAALNRRSDLTQVPELADYLKYMKPKKLAAFKGFKRAFFSFRDLYLSYHQSSSDVNSAPLGHFSLKGCEVSQDVSVGQQKYHIKLLLPTAEGMIDFILKCDSEHQYARWMAACRLASRGKSMADSSYQQEVESIKNLLKMQSGNGNENGNSNTASRKAAAVKLPNDFNVDEYISSKYVRRARSKQQIQQRVSDAHGNVRQLTATEAKLQYIRAWQALPEHGIHYN",
    "response#2": "TPMHKEARIQLPDMQMIDARVDFSVNSFKATKKLCRDLGIRYSEELSLKRYIPPEDLRRGTSDADNMNGPLSMRPGEESVGPMTLRKAAPIFASQSNLDMRRRGQSPALSQSGHIFNAHEMGTLPRHGTLPRGVSPSPGAYNDTMRRTPIMPSISFSEGLENEQFDDALIHSPRLAPSRDTPVFRPQNYVEKAAINRGWLDSSRSLMEQGIFEGDIILLRFKFMNFFDLNPKYDPVRINQLYEQAKWSILLDEFDHTEEEATLFAALQLQATLQRDSPEPEENNKDDVDILLDELEQNLDAAALNRRSDLTQVPELADYLKYMKPKKLAAFKGFKRAFFSFRDLYLSYHQSSSDVNSAPLGHFSLKGCEVSQDVSVGQQKYHIKLLLPTAEGMIDFILKCDSEHQYARWMAACRLASRGKSMADSSYQQEVESIKNLLKMQSGNGNENGNSNTASRKAAAVKLPNDFNVDEYISSKYVRRARSKQQIQQRVSDAHGNVRQLTATEAKLQYIRAWQALPEHGIHYR",
    "response#3": "ARPVLHVSQEIFIDPRAIDLEQPNCADGRLVCVHVKVCFSYVAVPSSYSPIVVLDYVLDGDTDRRLRGQAPRVTFPGRGPDDLKHQSSGTVSLKHQHDRVCGDTVFQLQENVKDKLRAIVVTLSYGLQTPRLRRQAPDQGLPLVAPILNAHQPSTQRTEIHFLKC"
  },
  {
    "reference": "MARHSGTSEGPKKRGRTELRSIRRPNRSQHSKHDQQVVHESENEDSLESSSDDDEDIAQAEAENSDRKEKVYGALLTILNSEHPERKKHKSHSTDFVKEIDEQEEIEQGMASESEEEDEENDEEESNEGDEEEEEEPTDQLDTFESHFNDVSQDLVDDISAGFKDKQIKYKSMKYSLDKKESAIFGKPLLLTQQDETIDNPVLTSSYDSYFIKQRLKIQNDLLDSSKENLTPLKKKLLDPMFQYKDVLCEYTNYENDEKEYRELYSLHVLNHIYKTRDKILKDNQRLQENDDLECLDQGFTRPKVLIVVPTRDTAYQVVETIIEKSGLDQIDKKGKFKDQFFDDSLPPTSKPKSFRHVFKGNTNDFFVLGMKFTRKAIKLYSNFYQSDIIICSPLGIQMILENTDKKKRQDDFLSSIEVMIVDQLHSIEYQNISHVYTILEHINKIPQQQREADFSRIRMWYINDQAKFLRQTMLFTRYISPTANAIINGKCHNMAGRWKNNQIISSEDSSIGQLGIKIKQIFQRFDLVGGTVVDESDYRFKYFTSVVMQNIVKSTGYEDGILVYIPEYTDYMRLRNYMKEKTTILFSEINEYSTQKQLDSNRSMFQQGRTKVLLYTERLHHYRRYELKGIKSVIFYKPPTNPEFYNEVIRFTAKNAFLGKSDINISTIRTVYSKLDGLALQRIVGSKRAAILCHGQNESYDFK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have U3 snoRNA binding and be active in nucleolus.2. The protein should exhibit Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "ILSLGAIFRLISEHNFEYPKFYDKVYSLTNPSLLYMSQKESILTLLDSFLSSTHLPTYITASFLKRLSRCLLLAPIDAQEPILGLIRNLVIRHPNCSELVHREVPQTLYDDPFDNDETDLHKTRALESSLWEMKLLQCHWNQSVRKRR",
    "response#2": "LLDDLDKELNTYAMRVREWFGWHFPELAKIVQDNILYAKAVKLMGNRINAAKLDFSEILADEIEAELKEAAVISMGTEVSDLDLLHIRELCDQVLSLAEYRAQLYDYLKSRMNTIAPNLTALVGELVGARLISHGGSLLNLAKQPGSTVQILGAEKALFRALKTKHATPKYGLIFHASVVGQAAPKNKGKISRSLAAKSVLAIRCDALGDSQDNTMGVENRLKLEARLV",
    "response#3": "PVPVKQTFSRFDSTSPVNDPENRFKYFTNAVLSPLVRSSAGGRGNPSASGTLIFIPSYLDFVRVRNYLATSSQTENLSFGAISEYTSVRDVARARTHFFSGRHSALLYTERTHHFRRYQIRGVKRVIMYGVPENPVFWAEIVGFLGLDPAGAAEAAEGGVRALFSKWDALKMERIVGTKRLGNMLIEKGGDTFTC"
  },
  {
    "reference": "MAPGYQPPSPEEKEQYVRELFDKIAENYDAMNQVMSAGQWEKWHREFVAQTHFRPGDHILDVACGTGDLTLLDAAQVAPDGKVIGVDISEGMLEVGRRRVAASPYKDLITLQLGNAMDLPFPDNTFDGVTMGWAMRNVASIPRTLSEIYRVLKPGGRFICLEASKPFSRFIRFGFFVYWKTFLPLIDWFVVKAGRQAKVRPYTYLSRSLDNYPFPDQLEELFREAGFVETDYQLLMLGTVAIHVGTKRREG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2)..2. The protein must exhibit demethylmenaquinone methyltransferase activity.3. The S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MPQYRSKTEA",
    "response#2": "LCTIYS",
    "response#3": "MTEKLIVGLTGGIGSGKSTVGALLADRGAFVVDTDA"
  },
  {
    "reference": "MRVKGIRKNCQHWWTWGIMLLGMLMICSTTEKLWVTVYYGVPVWKEATTTLFCASDAKAYDTEAHNVWATHACVPTDPNPQEVELVNVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCIDWKSNCTNNNDTQNNSNNSTQSDNCSGNTNNTHSNSSLGRIDAGEVKNCSFNVTTGIRDKIKKEYALFYTLDIMRVNNTSYTLINCNTSVIKQACPKTSFEPIPIHYCTPAGFAILKCNDKKFNGTGLCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSANFSNNAKNIIVQLNESIPINCTRPHNNTRRSISFTPGSAFVATENIIGDIRQAHCNISRERWNNTLGKIVTKLREQFRNKTIAFRPPIGGDPEIVMHSFNCGGEFFYCNTTQLFDSTWLSNGTRNDTTESDSTEIILQCRIKQIINRWQEVGKAMYAPPISGQIRCTSNITGMIITRDGGTNDTHGDEIFRPAGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQRGKRAVGTLGAMFLGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQNNMLKAIEAQQHMLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTAVPWNTSWSNKSLNTIWDNMTWMQWEREIDNYTGIIYNLLEESQNQQEKNEKDLLELDKWDSLWSWFDISQWLWYIKIFIMIVGGLIGLRIIFTVLSIVNRVRQGYSPLSFQTRLPTPRGPDRPEGIEEEGGERDRDRSGRLV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Incorporate a signal peptide in the protein design.2. The protein design should include a YXXL motif; contains endocytosis signal motif to ensure proper function.3. The protein should contain a Helical membrane-spanning region.4. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.5. The protein must exhibit the following characteristics: Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane.6. The designed protein have Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains with specific sequence motifs that are crucial for its activity.7. The protein should be optimized for stability and solubility when expressed in virion membrane, host cell plasma membrane, viral envelope where it will be involved in viral protein processing, positive regulation of receptor clustering. The designed protein sequence is ",
    "response#1": "MRVRGILRNYQQWWIWGILGFWMLMICNVVGNLWVTVYYGVPVWKEANTTLFCASDAKAYDTEVHNVWATHACVPTDPDPQEILLENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNANVTNNVTMDNNYKEEMQNCSFNVTTELRDKKQKAYSLFYKLDIVPLEGNTSSDSTSREYRLINCNTSAITQACPKVSFDPIPIHYCAPAGYAILKCNNKTFNGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIIRSENLTNNVKTIIVHLNESVAITCTRPNNNTRQSVRIGPGQVFYTNDIIGDIRQAYCNISKEKWNQTLQQVTEKLEELFPNKTIKFEPAAGGDLEITTHSFNCKGEFFYCNTSKLFNSPNDTESQPNYTIIPCRIKQIINMWQAVGRAMYAPPIAGNITCASNITGLLLSRDGGNTNTNGTSNTETFRPGGGDMRDNWRSELYKYKVVEIKPLGIAPTGAKRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASITVTVQARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLQDQRLLGIWGCSGRLICPXNVPWNSSWSNKSLGDIWDNMTWMQWEREIDNYTQEIYSLLEKSQNQQELNEQDLLKLDQWASLWNWFDITSWLWYIKIFIMIVGGLIGLRIVFAVLSIVNRVRQGYSPLSFQTLTPNPRGPDRLGGIEEEGGEQDRDRSVRLVNGFLALAWDDLRSLCLFSYH",
    "response#2": "MRVMETRMNCQWLWRWGTMILGMLMICSAAEENKWVTVYYGVSVWKEAKTTLFCASDAKAYEREVHNIWATHACVPTDPNPQELVLKNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNANSTTKTLWVTVYYGVPVWKEATTTLFCASDAKAYDTEAHNVWATHACVPTDPNPQEVELTNVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDWKCXNDSXXXXXXXXXXXXXXXXXXXGEIKNCSFNITTGISGKFQKEYALFHKLDVEPIXNDNASYRVISCNTSVITQACPKISFEPIPIHYCAPAGFALLKCNXKTFDGXGXCTNVSTVQCTHGIRPVXSTQLLLNGSLAEEXXIIRSANFSDNAKTIIVQLXTSVXINCTRPXNNTRKSIHLGQGRAWYTTGEIIGNIRQAXCNISKXEWTNTLXXVVXKLREQFKNKTIVFNQSAGGDPEIVMHTFNCGGEFFYCNTTQLFNTXWNXTXERINNTGXNETIILPCRIKQFINMWQQVGKAIYAPPIRGLIRCSSDITGLILTRDGXNNGNETNEVFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAIGLGAAFLGFLGTAGSTMGAASLTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTAVPWNASWSNKTYNQIWDNMTWMQWEREIDNYTSIIYDLIEKSQNQQEKNEQDLLELDKWASLWNWFEITSWLWYIKIFIMIVGGIVGLRIIFTVLSIVNRVRQGYSPLSFQ",
    "response#3": "MRVKGIQRNWKQWWTWGILGFWMLMICNVVGNKWVTVYYGVSVWKEAKTTLFCASDAKAYEREVHNIWATHACVPTDPNPQELVLKNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNANSTTKTLWVTVYYGVPVWKEANTTLFCASDAKAYDTEAHNVWATHACVPTDPNPQEVVLANVTEYFNMWKSNMVEQMHEDIISLWDQSLKPCVKLTPFCVTLNCSNATYTNSSENAKATPTPKTIVDPEMKNCSFNITTSMRDKMTKEQALFYALDVVPMDNDNDTIRDNGTASYRLRSCNTSVVTQACPKITFEPIPIHYCAPAGFALLKCNNKTFSGKGPCTNVSTVQCTHGIKPVVSTQLLLSGSLAEGEVILRSDNFTNNAKTIIVQLSTSVMIKCSRPSNNTSKSIHMGPGRAFYATERVIGNIRQAHCNLTRADWNRTLEKVVKKLGEQFENKTRISFNQPAGGDPEIVMHSFNCEGEFFYCNTTQLFNSTWVRDDANSSWRLNSSEGNDTLIILQCRIKQIINMWQEVGRAMYAPPIRGNISCLSNITGLILTRDGGIQNESQSQNGSESEETFRPVGGDMRDNWRSELYKYKVVKIEPLGVAPTRAKRRVVAREKRAVGIGAVFLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARILAVERYLRDQQLLGIWGCSGKLICTTAVPWNASWSNRSVDYIWENMTWMEWEKEIDNYTNLIYTLLDHAQNQQEKNEQELLELDKWASLWNWFDITKWLWYIKIFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQT"
  },
  {
    "reference": "MGRSLKKGPYVDKKLLNKIKEMNESGKKKVIKTWSRDSTIFPEMVGHTIAVHDGQKHVPVYITEDMVGHKLGEFAPTRKFRGHGAHTERSTALK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA..2. The ideal protein would be one that plays a critical role in translation through structural constituent of ribosome, rRNA binding within small ribosomal subunit. The designed protein sequence is ",
    "response#1": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#2": "MKPRFPIGCRVTLRGERMYDFLERLIFIALPRVRDFQGIPRRGFDGNGNYNLGIKEHTIFPEISFDKTDAVKGLNITIVTTADNDDMARTLLERVGLPH",
    "response#3": "MSPIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTQ"
  },
  {
    "reference": "MNSLLFVYGTLRKHEKNHHLLAQSACINEQARTKGSLFAAKEGPTVVFNDEDEGYIYGEVYEADELCIHKLDQFFQGYHKQTVFVETDVGIKIALIYFMNKDGCAGFTKISSGDWKEHQMISKSKNPIYYFAYGSCMDNARFQKAGVDHYFQDPVGRAVLKGYTTRFTLKREDGSRADMLEDGGTTEGVLYRIPYSALSYLYKREGVESLTYRPAFVDVEAGGRHYKDCLTFLVLQKEAEIAPPQHYQIEIERGAELYLSPEFTEKLKRHMNSLPKG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be optimized for cytosol-specific folding and stability, while maintaining gamma-glutamylaminecyclotransferase activity.2. The designed protein should have a high specificity for substrate binding, with minimal off-target effects.3. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "EDSYISIVEALRHGGIAHRCDVVIAKVDSEGLEALSEADVATRLAGFDGILVCPGFGARGVEGKVKAIRYAREQRIPFLGDCYGMQWAVIEFARHVCALPGANTTEVDPATPHPVIHLLPEQQGITEKGGTMRLGRYPCRLVPGSLAGSAYGEAEVGERHRHRYEVNNAYLDVLTRHGLRVTGVYPAKNLVEIIELPSHPWFVGTQFHGEYRSRPNRPHPLYREFL",
    "response#2": "EDSYISIVEALRHGGIAHRCDVVIAKVDSEGLEALSEADVATRLAGFDGILVCPGFGARGVEGKVKAIRYAREQRIPFLGDCYGMQWAVIEFARHVCALPGANTTEVDPATPHPVIHLLPEQQGITEKGGTMRLGRYPCRLVPGSLAGSAYGEAEVGERHRHRYEVNNAYLDVLTRHGLRVTGVYPAKNLVEIIELPSHPWFVGTQFHGEYRSRPNRPHPLYREFL",
    "response#3": "EDSYISIVEALRHGGIAHRCDVVIAKVDSEGLEALSEADVATRLAGFDGILVCPGFGARGVEGKVKAIRYAREQRIPFLGDCYGMQWAVIEFARHVCALPGANTTEVDPATPHPVIHLLPEQQGITEKGGTMRLGRYPCRLVPGSLAGSAYGEAEVGERHRHRYEVNNAYLDVLTRHGLRVTGVYPAKNLVEIIELPSHPWFVGTQFHGEYRSRPNRPHPLYREFL"
  },
  {
    "reference": "MTAEIKTGEKMIVFMVNGKEYAISVTQVKSIEKWQKPTRVPGVEPYICGVINLRGVVTPVIDLRKRLNLPEYEITDETRIIIIAYRDIEVGWIVDEANDVITVHESEIESAPEGVQKDTDVSIEQIVKQENRLLNIIDANAVLDKESSQSAVPDQA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must have at least one CheW-like domain.2. The protein should be designed to localize specifically to cytosol in order to perform its role in signal transduction, chemotaxis to oxidizable substrate, chemotaxis more effectively.3. For general function, the protein need meet that CheV and CheW are involved in the coupling of the methyl-accepting chemoreceptors to the central two- component kinase CheA; they are both necessary for efficient chemotaxis.; Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors The designed protein sequence is ",
    "response#1": "LVCIGVSTGGTEALRDVLECLPAKAPAILIVQHMPQGFTAAFAKRLDSLCEIEVREAQDGDVVVQGCAYIAPGGRHMLLQRTGQRYHIAIKDGPPVSRHRPSADVLFRSAAQYAGPNALGIIMTGMGDDGARGLLEMHKLGATTRAQDEESCVVFGMPKEAIALGGVDKVVPLAQIPREIL",
    "response#2": "PGPAPTRGQPTLVAIGASAGGPTALTALLRALPADFPAAIVIVQHVDQAFAYGMAEWLDGYTRLPVRVARQGSVPEAGAVLLAATNDHLMLSPRGVLGYTRHPAETPYRPSIDVFFNSVADGWQGDALGVLLTGMGRDGALGLKAMRAKGCYTIAQDQATSAVYGMPKAAAAIGAASAILPLDQIAPQLIARIALFRVGRKIVAIGSSTGGVEALIAVLQKFPANCPPTVITQHMPPTFTKSFAERLNRLCAPVVQEATDGARLEIGKIYLAPGGERHLQVSGASAPCCRLIDRAPVNGHRPSVDVLFDSVAELAGRNAVGVILTGMGRDGAAGLLKMRHAGARTLGQNEKTCVVYGMPRVAHELGAVEQQLPLSAIGEEILKMTAAR",
    "response#3": "VCIGVSTGGTEALRDVLECLPAKAPAILIVQHMPQGFTAAFAKRLDSLCEIEVREAQDGDVVVQGCAYIAPGGRHMLLQRTGQRYHIAIKDGPPVSRHRPSADVLFRSAAQYAGPNALGIIMTGMGDDGARGLLEMHKLGATTRAQDEESCVVFGMPKEAIALGGVDKVVPLAQIPRER"
  },
  {
    "reference": "MNYKNLMMWSVIVLLIVGLFQLYQNPKHSITSDKVPFSSFLKNLEENRVIEVEIKGNEIKGVLSDGTAFNTYAPNDPNLVEKLSSKGVNITASPQDDKMPSLLGILLSWFPMLLLIGVWIFFMRQMQGGKGGAMGFGRSKAKLLSEARGKVTFNDVAGIDEAKEEVEEMVEFLKDPRKFRXLGGKIPKGALLIGPPGTGKTLLARAIAGXADVPFFTISGSDFVEMXVGVGASRVRDMMXQGKKNAPCIIFIDXIDAVGRSXGAGLGGGNDEREQTLNQLLVEMDGFDTNEGVIIIAATNRPDVLDPALIRPGRFDRQVVIPNPDIVGREAILKIHIKKITTGPDVKLRTIARGTPGFSGADLANLTNEAALLAARKNKKIVTMTDIEEAKDKVMMGSERRSMVMTEEDKKLTAYHEGGHAIVALNEKASDPIHKATIIPRGRALGVVWTLPERDKYSHTREYLEANISKAMGGRVAEELIFGHAKVTSGASSDIQMATKLAKDMVTKFGMSEKLGPLTYGENEDEVFLGRSITRQQHISEETAKKVDFEIKRIVDEGYQKAKKILTEKIDDLHKLAKVLILYETLSGDEINDLIIKNTQPKRENEKDEETKEESSSLGSLGLKPKPAV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. The designed protein should contain one or more AAA+ ATPase domains that are essential for its function.3. The protein design should emphasize Basic and acidic residues compositional bias.4. The protein must bind to Zn(2+) in order to perform its enzymatic function.5. A protein with ATP hydrolysis activity, zinc ion binding, metalloendopeptidase activity, ATP-dependent peptidase activity, ATP binding functions is required to enable proteolysis, cell division.6. For general function, the protein need meet that Plays a role in the quality control of integral membrane proteins.; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins7. The designed protein should have a binding site that is specific to the target Zn(2+), ATP ligand.8. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "MVMSEDEKAMTAYHEAGHAIVGRMVPEHDPVYKVSIIPRGRALGVTMYLPEQDKYSHSKQHLESMISSLFGGRIAEAIIYGNDKVTTGASNDIERATDIARKMVTQWGLSEKMGPMLYAEEEGEVFLGRSMARAKHMSDDTARAIDAEIKAVIDRNYERAEQILRDNIDILHTMKDALMKYETIDARQIDAQRAASR",
    "response#2": "MVMSEKEKLNTAYHESGHAIVGRLMPEHDPVYKVSIIPRGRALGVTMFLPEEDKYSHSKRYLDSSICSLFGGRIAEELTLGFEGVTTGASNDIERATSLARNMVTRWGLSEKLGPLQYDTDSDEPFLGRSAGQAQTVYSPETAQRIDEEVRNIIDTCYEKAKQILVDNRDKLDLMADALMKYETIDRFQIDDIRKLYEEE",
    "response#3": "MVMSEKEKLNTAYHESGHAIVGRLMPEHDPVYKVSIIPRGRALGVTMFLPEEDKYSHSKRYLDSSICSLFGGRIAEELTLGFEGVTTGASNDIERATSLARNMVTRWGLSEKLGPLQYDTDSDEPFLGRSAGQAQTVYSPETAQRIDEEVRNIIDTCYEKAKQILVDNRDKLDLMADALMKYETIDRFQIDDECAAHWQELERRAAG"
  },
  {
    "reference": "MSGQLTLDDLRALDERDPLASRRARFRLPADGVYLDGNSLGALPRRTPAAVADLVENQWGRDLISSWNGHDWIGMPQRLGGLIAPLIGARASEVIACDTTSTNLFKLICAAMTAQPGRPVVLSEPGNFPTNLYMAQGAVGLLGDRVLRTVPVDEIAGAIDESTALVMLTHVHYKSGRKLDMKAFTAAAHAKGALILWDLSHSAGAVPLWLDRNDVDLAVGCGYKYLNGGPGAPAFLFVADRLQERLHSPLPGWMGHDAPFAFEDEFRPSAGILRFLCGTPSVIGMAALESGLKEFDDVDLDALHAKATALGGLFIRLMDQRCDGFGFELASPRRGESRGSHVSYAHPEAYPICQALIARGVVGDFRAPDLLRMGFAPLYTSYADVWVAVETLHAVMHERAWDSDMFRTRARVT",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be able to bind pyridoxal 5'-phosphate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "KDVVKLASNENPIGTNPAITKVIRSNADIAHLYPDDSMFELKEALSRKFDVEDENIIIGAGSDQVLEFISRALLNEASSVLMSAVTFAMYEIYAKQMGAKIIRTESYEHKYDEFIEAYHTYKPKIIYICTPNNPTGDATSREEVLKIIEGVDKDTLVVVDGAYMEYAAAKDQKHAIAPTDLLKYENVIYLGTFSKAHGLGGMRVGYGIAQAKLIKELYKMRPPFNITTLSLLVAIEACKDDSFVEASIALHQEQIKRYETFAKENGISYIESYTNFITYLFDENLDSTQISDALLKRGVIIRNLASYGMNGVRITIGTEVQNDVFFA",
    "response#2": "LKDTDPEVDQIIKDEIDRQQHSIVLIASENFTTTAVFDALGTPMCNKYSEGYPGARYYGGNEHIDRMELLCQERALKAFGLTPDKWGVNVQTLSGSPANLQVYQAIMKPHERLMGLDLPHGGHLSHGYQTDSRKISAVSTYFETMPYRVDLETGLIDYDMLEKTAVLYRPKVLVAGTSAYCRLIDYKRMREIADKVGAYLVVDMAHISGLIAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRRGVRSVNPKTGQEILYDLENPINFSVFPGHQGGPHNHTIAALATALKQANTPEFKEYQEQVLKNAKALESEFTKKGYKLVSDGTDSHMVLVSLKDKQIDGARVETVCEKINIALNKNSIPGDKSALVPGGVRIGAPAMTTRGLGEEDFKKIVSYISQKDAAEIDEALMGPLGFSVDQLMELAGLSVAAAIAEVYKLSEYSRVLAICGPGNNGGDGLVAARHLYHFGYKPFISYPKRTSKPLYSGLVTQLESLSIPFLSVEDLPQNLSDDFDIIVDAMFGFSFHGTPRPPFDDLIQRLVALKAFDNAPKKSPPIVSVDIPSGWHVEEGDINGEGIKPEMLVSLTAPKLCAKKVAEEL",
    "response#3": "LKDTDPEVDQIIKDEIDRQQHSIVLIASENFTTTAVFDALGTPMCNKYSEGYPGARYYGGNEHIDRMELLCQERALKAFGLTPDKWGVNVQTLSGSPANLQVYQAIMKPHERLMGLDLPHGGHLSHGYQTDSRKISAVSTYFETMPYRVDLETGLIDYDMLEKTAVLYRPKVLVAGTSAYCRLIDYKRMREIADKVGAYLVVDMAHISGLIAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRRGVRSVNPKTGQEILYDLENPINFSVFPGHQGGPHNHTIAALATALKQANTPEFKEYQEQVLKNAKALESEFTKKGYKLVSDGTDSHMVLVSLKDKQIDGARVETVCEKINIALNKNSIPGDKSALVPGGVRIGAPAMTTRGLGEEDFKKIVSYIAEDVLKGFDTEAGLAKLRAQVAAL"
  },
  {
    "reference": "MKVKGIQRNYQHLWRWGILIFGMLMISRAAENLWVTVCYGVPVWKDAETTLFCASDAKAYSAEAHNVWATHACVPTDPNPQEIYLQNVTEDFNMWTNNMVEQMHEDIISLWEQSLKPCVKLTPLCVTLDCSNATYKNTTTHNGNSTTPSTPREDIKNCSYNVTTELRDKRQRVYSLFYRLDIVPIDESSNGRSNSSEYRLINCNTTVITRACPKISFEPIPIHYCAPAGFAILRCNNETFNGTGPCNNVSSVQCTHGIKPVVSTQLLLNGSLAKGGNIMIRSKNITDNSKNIIIQFTEPVPINCTRPNNNTRRSIRFGPGQAFYTNSIIGDIRQAFCSINKTKWNNTLQKVAEQLRRHFPNKTINFTSSSGGDIEITTHSFNCGGEFFYCNTTGLFNHTFNSTWGNATITENANETIILPCRIKQIINMWQEVGQAMYAPPIEGVIKCISNITGIILTRDGGHMSNGSNDSIETFRPGGGNMRDNWRSELYKYKVVKLEPLGVAPTRARRRVVEREKRAVGLGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLKAIDAQQQMLRLTVWGIKQLQARVLAVERYLQDQQLLGLWGCSGKLICTTFVPWNSSWSNKTQDEIWENMTWLQWDKEISNYTGTIYNLLEIAQNQQEKNEQDLLALDKWANLWNWFNISNWLWYIRIFIMIVGGLIGLRIVFAILSVVKRARQGYSPVSFQIPTPSPAGLDRLGRIEEGSGEQDRDRSIRLVSGFLALAWDDLR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be designed to localize specifically to viral envelope, host cell plasma membrane, host cell endosome membrane, plasma membrane, virion membrane in order to perform its role in viral protein processing, positive regulation of receptor clustering, clathrin-dependent endocytosis of virus by host cell, virion attachment to host cell, fusion of virus membrane with host plasma membrane more effectively.2. For general function, the protein need meet that The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Transmembrane protein gp41: Acts as a class I viral fusion protein; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state3. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "MWVTVYYGVPVWRDADTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVKQMHKDIISLWDQSLQPCVKLTPLCVTLNCNRVNNSSSSNSTNNXXTSSNSTVSPGIKXEMMNCSFNMTXXVXXKKXKVSALFYRLDVXQIDSEQTNSTXYRLINCNTSTVTQACPKVTFEPIPIHYCAPAGFAILKCKDKNFTGTGPCXNVSTVQCTHGIRPVXTTQLLLNGSLAEERVMIRSENLTNNTXTILVQFQNPVRINCTRPSNNTRKSLRIGPGQTFYATGEVIGDIRRAHCNVSTQDWNDTLKEVVTQLRGHFKNKTIIFKNHAGGDLEIVTHSFNCGGEFFYCNTTDLFNSTWDKDGNGTVSSGSNGTITLQCRIKQIINMWQRVGQAMYAPPIQGVIRCDSNITGLLLTRDGGNGNDSQANETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVQREKRXLGAAXLGFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLRDQQLLGIWGCSGKLVCTTAVPWNSSWSNKSKTEIWDNMTWMQWDKEISNYTNTIYKLLEDSQIQQENNEKDLLALDKWNNLWSWFNITNWLWYIRIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLLFQTLLP",
    "response#2": "MRVKETQMNWPNLWKWGTLILGLVIICSASDNLWVTVYYGVPVWKDATTPLFCASDAKAYVTEGHNVWATHACVPTDPNPQEVVMENVTENFNMWKNDMVEQMHEDVISLWDESLKPCVKLTPLCVTMNCTDANLNTNSSINNSSLIKMKEEIKNCSFNITSQIKNTVRKDYALFNKLDIVPIENDNSSYMLINCNTSVITQACPKVSFEPIPIHYCTPAGFAIIKCNEKGFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEGDIVIKSENFTNNAKTIIVQLNETVIINCTRPNNNTRKSIHVGWGRSLYATGDIIGNIRQAHCNVSAERWSNILKQVAIKLKDQFKPKTIDFKQPTGGDPEIVMHTFNCGGEFFYCNTSKLFNYTLTNNTWNNQTVNGTDVITLPCRIKQIINRWQEVGKAMYAPPIRGQINCTSNITGLLLTRDGGNGNETGSNRSEIFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRAVGIGAMIFGFLGAAGSTMGAASITLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLGDQQLLGIWGCSGKLICTTAVPWNASWSNKSLNDIWDNMTWMQWDREISNYTSKIYKLIEKSQNQQEKNEQELLELDKWASLWDWFSITNWLWYIRLFIMIVGGLVGLRIIFAVLSIVNRVRQGYSPLSFQTHLPVPRGPDRPEGIEEEGGERDRD",
    "response#3": "MRVMETRKNYQHLWKWGTLLLGMLMICSAAENLWVTVYYGVPVWRDADTTLFCASDAKAYDKEVHNVWATHACVPTDPNPQEISLENVTEEFNMWKNNMVEQMQTDIISLWDQSLKPCVKLTPLCVTLNCNNPNSSSTSFNATNGTISSGEGELKNCSFNITSSIRNKMKKEYALFYTLDIIPIDDDSNTTDSNTTYRLRSCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSKNFSDNAKTIIVQLNESVVINCTRPNNNTRKSITIGPGRAFYTTGQIIGDIRQAHCNLSRTQWNNTLKQIVGKLREQFGNNKTIVFNQPSGGDPEIEMHSFNCGGEFFYCNTTQLFNSTWIFNSTWNDTNTEGNDTITLQCRIKQFINMWQEVGKAMYAPPISGQIRCSSNITGLLLTRDGGINESRTNETFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRAVGLGALFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTTVPWNSSWSNKSLSEIWDNMTWMQWDKEVSNYTNIIYDLIEKSQNQQEMNEQDLLALDKWAGLWXWFDISNWLWYIKIFIXIVGGLIGLRIVFALLHIINRARQGYSPLSLQTLLPAQRGPDRPEGI"
  },
  {
    "reference": "MGNRGMEDLIPLVNRLQDAFSAIGQNANLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLVLQLVNANTEYGEFLHCKGKKFTEFDEIRQEIEAETDRVTGSNKGISAVPINLRVYSPNVLSLTLVDLPGMTKVPVGDQPSDIEFQIRDMLMQFVTKENCLILAVSPANSDLANSDALKIAKEVDPQGQRTIGVITKLDLMDEGTDARDILENKLLPLRRGYVGVVNRSQKDIDGKKDIQAALAAERKFFLTHPAYRHMADRMGTPYLQKVLNQQLTNHIRDTLPGLRNKLQSQLLSIEKEVEEYKNFRPDDPARKTKALLQMVQQFAVDFEKRIEGSGDQIDTYELSGGARINRIFHERFPFELVKMEFDEKELRREISYAIKNIHGIRTGLFTPDLAFEAIVKKQVQKLKEPCLKCVDMVVSELTSTIRKCSNKLSQYPHLREEMERIVTTHIRDREGKTKDQVMLLIDIELAYMNTNHEDFIGFANAQQRSSQMSKKKTAGNQVSIVCCRSEKEKKYMLPVDNLKVRDIEKSFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFLRAGVYPERVVSGDSEENGSDSLMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMINNTKDFIHSELLANLYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALNIIGDINTTTISTPLPPPVDDSWLQVSPTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGGPTLGGAP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. We need a protein that can perform GTP + H2O = GDP + H(+) + phosphate with high efficiency.2. The protein contains novel PH domains that confer a unique function or activity.3. The ideal protein would be one that plays a critical role in modulation of chemical synaptic transmission, receptor internalization through GTPase activity, GTP binding within photoreceptor ribbon synapse. The designed protein sequence is ",
    "response#1": "MEELIPLVNKLQDAFSSIGQSCHLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLILQLIFSKTEHAEFLHCKSKKFTDFDEVRQEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLIDLPGITKVPVGDQPPDIEYQIKDMILQFISRESSLILAVTPANMDLANSDALKLAKEVDPQGLRTIGVITKLDLMDEGTDARDVLENKLLPLRRGYIGVVNRSQKDIEGKIVLTGGPCGGKTTGQSRLCTFFENLGWKVFRVPETATVLLSGGVKFSDLTEKEAYKFQENLIRTMVQIENTYFELGNSSNRNCLIICDRGVMDASAYISKDKWEKMMAGNNWNPVEMRDNRYNQILHLVSAANGAEDFYSTEDHACRSEGVDLARELDYKSAAAWVGHPYFDVIDNSTNFELDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLILQLIFSKTEHAEFLHCKSKKFTDFDEVRQEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLIDLPGITKVPVGDQPPDIEYQIKDMILQFISRESSLILAVTPANMDLANSDALKLAKEVDPQGLRTIGVITKLDLMDEGTDARDVLENKLLPLRRGYIGVVNRSQKDIEGKKDIRAALAAERKFFLSHPAYRHMADRMGTPHLQKTLNQQLTNHIRESLWEALPTVAVVGGQSSGKSSVLESIVGRDFLPRGSGIVTRRPLVLQLHKTDDGTEEYAEFLHLPKKQFTDFALVRREIQDETDRITGKNKQISPVPIHLSIYSPNVVNLTLIDLPGLTKVAVEGQPETIAEDIESMVRTYVDKPNCIILAISPANQDIATSDAIKLAKDVDPTGERTFGVLTKLDLMDKGTNALEVLEGRSYRLQHPWVGIVNRSQADINKNVDMMLARRKEREYFDTSPDYGHLASKMGSEYLAKLLSKHLESVIRTRI",
    "response#2": "NALEVLEGRSYRLQHPWVGIVNRSQADINKNVDMMLARRKEREYFDTSPDYGHLASKMGSEYLAKLLSKHLESVIRTRIPSILSLINKSIEELERELDRMGRPVAVDAGAQLYTILEMCRAFDKIFKEHLDGGRPGGDRIYGVFDNQLPAALKKLPFDRHLSLQSVKKIVSEADGYQPHLIAPEQGYRRLIEGALGYFRGPAEASVDAVHYVLKELVRKSISETEELKRFPSLQVELAAAANSSLEKFREESKKSVIRLVDMESAYLA",
    "response#3": "LPTVAVVGGQSSGKSSVLESIVGRDFLPRGSGIVTRRPLVLQLHKTDDGTEEYAEFLHLPKKQFTDFALVRREIQDETDRITGKNKQISPVPIHLSIYSPNVVNLTLIDLPGLTKVAVEGQPETIAEDIESMVRTYVDKPNCIILAISPANQDIATSDAIKLAKDVDPTGERTFGVLTKLDLMDKGTNALEVLEGRSYRLQHPWVGIVNRSQADINKNVDMMLARRKEREYFDTSPDYGHLASKMGSEYLAKLLSKHLESVIRTRIPLVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVEWEALPTVAVVGGQSSGKSSVLESIVGRDFLPRGSGIVTRRPLVLQLHKTDDGTEEYAEFLHLPKKQFTDFALVRREIQDETDRITGKNKQISPVPIHLSIYSPNVVNLTLIDLPGLTKVAVEGQPETIAEDIESMVRTYVDKPNCIILAISPANQDIATSDAIKLAKDVDPTGERTFGVLTKLDLMDKGTNALEVLEGRSYRLQHPWVGIVNRSQADINKNVDMMLARRKEREYFDTSPDYGHLASKMGSEYLAKLLSKHLESVIRTRIDARDVLENKLLPLRRGYIGVVNRSQKDIEGKKDIRAALAAERKFFLSHPAYRHMADRMGTPHLQKTLNQQLTNHIRESLPALRSKLQSQLLSLEKEVEEYKNFRPDDPTRKTKALLQMVQQFGVDFEKRIEGSGDQVDTLELSGGARINRIFHERFPFELVKMEFDEKDLRREISYAIKNIHGVRTGLFTPDLAFEAIVKKQVVKLKEPCLKCVDLVIQELINTVRQCTSKLSSYPRLREETERIVTTYIREREGRTKDQILLLIDIEQSYINTNHEDFIGFANAQ"
  },
  {
    "reference": "MTAIDKIQVLDSIEKELLLCLQSAGQALLELSKEKTSQKATETHTNQFLKSLNIVESKLSEQINYLTQVSTGQPHEGSGYAAAKVLQMAWHRIQHVKSRIKELEECKIKYVQATNRLQTQRTGPNPGSGGVM",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have transcription coregulator activity within mediator complex to regulate regulation of transcription by RNA polymerase II.2. The protein must exhibit the following characteristics: Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors .; Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery; Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. The designed protein sequence is ",
    "response#1": "LIGLLKDYKPGTITIENITKLCQSMGLESFIDELDNDISRLSTASKIIVIDIDYNKTQSTVQDVKLVLASNFDNFDYSNEQLEEAGDKQSNILLNSLTNYDSLKQFHHNLEFLYLLDTYSSC",
    "response#2": "LIGLLKDYKPGTITIENITKLCQSMGLESFIDELDNDISRLSTASKIIVIDIDYNKTQSTVQDVKLVLASNFDNFDYSNEQLEEAGDKQSNILLNSLTNYDSLKQFHHNLEFLYLLDTYSSC",
    "response#3": "LIGLLKDYKPGTITIENITKLCQSMGLESFIDELDNDISRLSTASKIIVIDIDYNKTQSTVQDVKLVLASNFDNFDYSNEQLEEAGDKQSNILLNSLTNYDSLKQFHHNLEFLYLLDTYSSG"
  },
  {
    "reference": "MVLASISEALISQGDGGERVAKRQRSAIVLLDLDILDCPICCEALTSPIFQCDNGHLACGSCCPKLSNKCPACTLPVGHSRSRAMESVLESILIPCPNVRFGCTKSFFYGKESAHEKECIFSQCSCPSSVCDYTGSYKDLYAHYKLTHSTNIFWNIKRFRCANFFTTSMLISDKILIKRVHEKKLLLAVQCFREPCGVYVTVSFIAPSAPEVGEFSYQLSYNVDGHTVTYESPEVKRVCKVSIETPQENFMLIPHSLLRGDLLEMQVFIIENVDQE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have transferase activity, zinc ion binding to facilitate protein ubiquitination, ubiquitin-dependent protein catabolic process.2. We require a protein that can specifically bind to the metabolites produced in the protein ubiquitination, Protein modification pathway.3. For general function, the protein need meet that E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins; It probably triggers the ubiquitin-mediated degradation of different substrates. The designed protein sequence is ",
    "response#1": "LTTAAPGYTCPLQGSHAICTCCFQPMPDRRAEHEQDSRIAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLTTRGLTWKNMLTESLLALQRGVFILSDYRITGNTVLCYCCGLRSFRELTYQYRQNC",
    "response#2": "LTTAAPGYTCPLQGSHAICTCCFQPMPDRRAEHEQDSRIAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLTTRGLTWKNMLTESLLALQRGVFILSDYRITGNTVLCYCCGLRSFRELTYQYRQNC",
    "response#3": "LTTAAPGYTCPLQGSHAICTCCFQPMPDRRAEHEQDSRIAPQQCAVCLQPFCHLYWGCTRTGCFGCLAPFCELNLGDKCLDGVLNNNNYESDILKNYLTTRGLTWKNMLTESLLALQRGVFILSDYRITGNTVLCYCCGLRSFRELTYQYRQNC"
  },
  {
    "reference": "KRLLQDLNIKVNKVIPEGGSVKDLQDLPKAWFNLVPYREIGLMTAIYLEKNFGMPYISITPMGIVDTAEFIRQIEKHVNNLVSNKKFNYEPYIDQQTRFV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. We need a protein that can perform 2 ADP + chlorophyllide a + oxidized 2[4Fe-4S]-[ferredoxin] + 2   phosphate = 2 ATP + 2 H2O + protochlorophyllide a + reduced 2[4Fe-   4S]-[ferredoxin] with high efficiency.2. The designed protein should interact with enzymes and substrates within the chlorophyll biosynthesis (light-independent) pathway to enhance or regulate its activity.3. The designed protein must be optimized for 4 iron, 4 sulfur cluster binding. The designed protein sequence is ",
    "response#1": "AHIGTLRVASSFKNVHAIMHAPLGDDYFNVMRSMLERERDFTPVTASIVDRHVLARGSQEKVVDNINRKDKEERPDLIVLTPTCTSSILQEDLQNFVDRASTTSNSDVILADVNHYRVNELQAADRTLEQVVRYYLEKARRQGTLDQSLTKEPSANIIGIFTLGFHNQHDCRELKRLLQDLNIKVNKVIPEGGSVKDLQSLPKAWFNLVPYREIGLMTAIYLEKNFGMPYVSITPMGIVDTAECIRQIQKHVNNLIPNKKVDYEPYIDQQTRFVSQAAWFSRSIDCQNLTGKKAVVFGDATHAASITRILAREMGIRVGCTGTYCKHDTEWFKEQVQGFCDEILTTDDHTEVGDMIARIEPSAIFGTQMERHIGKRLDIPCGVISSPVHIQNFPLGYRPFLGYEGTNQIADLIYNSGGMTVIE",
    "response#2": "AHIGTLRVASSFRNVHAIMHAPLGDDYFNVMRSMLERERDFTPVTASIVDRHVLARGSQEKVVENITRKDKEERPDLIVLTPTCTSSILQEDLQNFVDRASIASNSDVILADVNHYRVNELQAADRTLEQVVRYYLDKARRQGTLSQSITEKPSVNIIGMFTLGFHNQHDCKELKRLLQDLGIQVNEVIPEGGSVENLRNLPKAWLNLVPYREVGLMTALYLEKEFGMPYVATTPMGIVGTAEFVRQIQSHINKWAPMFLGKLVDYEPYIDQQTRFISQAAWFSRSIDCQNLTGKKVVVFGDTTHAASMTKILAREMGIHVVCAGTYCKHDADWFKEQVQGYCDEILVTDDHTQVGDMIARIEPAAIFGSQMERHIGKRLDIPCGVISAPVHIQNFPLGYRPFLGYEGTNQIADLVYNSGGLDKP",
    "response#3": "CPISCVAWLYQKIEDSFFLVIGTKTCGYFLQNALGVMIFAEPRYAMAELEEGDISAQLNDYEELKRLCFQIKKDRNPSVIIWIGTCTTEIIKMDLEGMAPKLEVELGVPIVVARANGLDYAFTQGEDTVLAAMAHRCPERDLLIDENKVIQNQTIQNIFSLFSQKKKEETLKYLELKNHPPLVLFGSLPSTVASQLSLELKRQSIQVSGWLPTQRYTDLPSLGDGVYVCGVNPFLSRTATTLIRRRKCKLIGAPFPIGPDGTRAWIEKISSVFGIKTKGLEEREQQVWQSLKNYLNLVRGKSVFFMGDNLLEISLARFLIRCGMIVYEIGIPYMDKRYQAAELALLRNTCREMCTPMPRIVEKPDNYNQIQRMRELQPDLAITGMAHANPLEARGINTKWSVEFTFAQIHGFTNAKDVLELVQ"
  },
  {
    "reference": "MSMSTHMPTPTPSDTKKMPPKEKKNKDDRPYKCTFCDKAFHRLEHQTRHIRTHTGEKPHACTFPGCVKRFSRSDELTRHLRIHTNPSSRKRKNKNQNMMINPNATTATTTGPAGMAIPTSNLPPGSYAIPNTAIPFSIDRNGNHVYHQPYPVYFVPHANGYMQPMVQTQGVTVVPNTDQQQHQQQQQYHQQQQQYQFQERPQHQQQQQTNIGTNNHLQQNGSAVFSLPSSPTMASAQTPNSGTSSSSESIKLAPLSIPNNISRPQQQQQQQQQQQQIPKSESSTSLYSDGNKLFSRPNSTLHSLGSSPENNNASGPLSGVPTPSFSNLNDYFQQKSNSNNSRLFNASSSSLSSLSGKIRSSSSTNLAGLQRLTPLTSTTNNTNNTTTSNTNNNNMTKPSIIPKQPSSTSLNLEFYNGNNQQQQNYHTHKKSRPNSPSQTPIHLSSSRKSANNSFIISPNETPLQTPLQSPQLKPYQDQPPTNVNINVSAPSDTFIGTAVTEKLNNISSIAGNGTQLPPIRSVLSFTSLVDYPDPKQKNVAPPVVDTTNVARGETNGSNGNNSNAKPMSLTNLLS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must be able to metal ion binding. The designed protein sequence is ",
    "response#1": "VRIGLVGKYVNLPDAYLSVAEALRHGGYDCGARVDIDWIASDDAEGLLAEGRLHELDGIVVPGGFGFRGIEGKIAAVGYARENDIPFLGLCLGMQCAVVEFARNVCDLAGANSSEFDPHTPHPVIDLMDEQRDVIDMGGTMRLGIYPARLEPGSIVSAAYGEEVIYERHRHRYEVSNKHRPRLEEMGLVCSGTSPDGRLVEFVELPTDKHPFFVATQAHPEFKSRPDRPHPLFAAFIAR",
    "response#2": "VRIGLVGKYVNLPDAYLSVAEALRHGGYDCGARVDIDWIASDDAEGLLAEGRLHELDGIVVPGGFGFRGIEGKIAAVGYARENDIPFLGLCLGMQCAVVEFARNVCDLAGANSSEFDPHTPHPVIDLMDEQRDVIDMGGTMRLGIYPARLEPGSIVSAAYGEEVIYERHRHRYEVSNKHRPRLEEMGLVCSGTSPDGRLVEFVELPTDKHPFFVATQAHPEFKSRPDRPHPLFAAFIHAN",
    "response#3": "VRIGLVGKYVNLPDAYLSVAEALRHGGYDCGARVDIDWIASDDAEGLLAEGRLHELDGIVVPGGFGFRGIEGKIAAVGYARENDIPFLGLCLGMQCAVVEFARNVCDLAGANSSEFDPHTPHPVIDLMDEQRDVIDMGGTMRLGIYPARLEPGSIVSAAYGEEVIYERHRHRYEVSNKHRPRLEEMGLVCSGTSPDGRLVEFVELPTDKHPFFVATQAHPEFKSRPDRPHPLFAAFVS"
  },
  {
    "reference": "MTTPTETYVDASGGDWDEVVAAAQRSQTDRLVINMGPQHPSTHGVLRLILTLDGETVTEARVGIGYLHTGIEKNMEYRTWTQGTTFVTRMDYLTPLFNETAYCLAVEKLLGITDQVPERANVIRVMMMELNRISSHFVAMATFGMELGATTVMTNGFREREMVLDIFEMVTGLRMNHAYIRPGGVAQDLPPGTVGKVRELLTEMPKRLKDMRKLLDANPIYLARTRDVAYLDVTGCLALGVTGPILRAAGLPWDLRKAKPYCGYENYEFDIPVSDGADVYARYRVRMAEIEQSLKIIEQCLDKLKPGPVMIEDPKIGWPAQLALGPDGMGNAPSHIAHIMGKGGSMEALIHHFKLVTEGFRVPPGQAYAAVESPKGELGCFAVSDGGPRPYRVHFRDPSFTNLQAVAAMCEGGTVADVIAAVASIDPVMGGVDR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone; Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.; NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain.2. The protein should have a fast turnover rate for a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) +   NAD(+).3. A protein that localizes to plasma membrane and can perform NAD binding, quinone binding, NADH dehydrogenase (quinone) activity with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "LGALTVMTIGFRERELVLDLFELITGLRMNHAFIRPGGVAQDMPPGALDEIRGFVALMKKRLPEYADLCNANPIFKGRLEGIGHLDLAGCLALGLTGPVLRSTGYPWDLRKTQPYCGYETYDFDVQTWDTSDSYGRFRIRLNEMWESLRIIEQAADRLAGLDGAPVMIEDKKIGWPSQLAIGSDGMGNSLDHIRHIMGESMEALIHHFKLVTEGFRVPPGQAYVPVESPRGELGAHVVSDGGTRPFRAHFRDPSFTNLQATSVMAEGGMVADVIVAIASIDPVMGGVDD",
    "response#2": "LGALTVMTIGFRERELVLDLFELITGLRMNHAFIRPGGVAQDMPPGALDEIRGFVALMKKRLPEYADLCNANPIFKGRLEGIGHLDLAGCLALGLTGPVLRSTGYPWDLRKTQPYCGYETYDFDVQTWDTSDSYGRFRIRLNEMWESLRIIEQAADRLAGLDGAPVMIEDKKIGWPSQLAIGSDGMGNSLDHIRHIMGESMEALIHHFKLVTEGFRVPPGQAYVPVESPRGELGAHVVSDGGTRPFRAHFRDPSFTNLQATSVMAEGGMVADVIVAIASIDPVMGGVDDLGGFSPLMYAFDDREQILDLLESITGSRLTYCYYRFGGLYNDIDDEFLVGTRKFIIRMRKSLKTYRDLVTNNIILMKRLKDIGHISPEICRKYGATGPVARGSGINFDVRKNEPYSVYNEFDFDIPVYHEGDSYARYMVRMDEMEQSLRIIEQAMDKLPGGPIIPEKKPKIIKLPEGDYYSTVEAARGSFGIRLVSDGGKNAYRLKLRSPTFSNMHLFDEVCQGMLIADALALMGSLDLVIPEIDDLGGFSPLMYAFDDREQILDLLESITGSRLTYCYYRFGGLYNDIDDEFLVGTRKFIIRMRKSLKTYRDLVTNNIILMKRLKDIGHISPEICRKYGATGPVARGSGINFDVRKNEPYSVYNEFDFDIPVYHEGDSYARYMVRMDEMEQSLRIIEQAMDKLPGGPIIPEKKPKIIKLPEGDYYSTVEAARGSFGIRLVSDGGKNAYRLKLRSPTFSNMHLFDEVCQGMLIADALALMGSLDLVIPEID",
    "response#3": "DVGAMTVFLYAFREREDLMDCYEAVSGARLHAAYYRPGGVYRDLPDNMPQYQPSAIHDEKATRARNENRQGSLLDFIEDFTRRFPGYIDDYEALLTDNRIWKQRLVDIGVVSPDRAKALGFTGPMLRGSGVEWDLRKKQPYEVYDQVDFDIPVGANGDCYDRYLVRIEEMRQSNHIIKQCVEWLRKNPGPVITDNHKVAPPSRLAMKQNMEEMIHHFKLFTEGMHVPRGEAYAAVEHPKGEFGIYIVSDGANKPYRLKIRAPGFAHLAALDEMTKGHMIADLVAIIGTQDIVFGEIDDLGAFTPFLFCFRDREHLLNLLEWICGARMLYNYIWVGGVSHDFPAKFEERVMEFVRYFKPQARELYQLLTENEIFVQRTKDIGLLPADVAINAGASGPMLRGSGVKWDLRRDDPYSIYPELEFDVAVPDGKASVIGDCLSRHLVRAIEIDESLKMIEQCIDMMPKDPNFDPHS"
  },
  {
    "reference": "MDYTPRLQPARLIKRYKRFLADVVTPEGETLTLHCANTGAMTGCATPGDTVWYSTSDNPKRKYPHSWELTETQQNHWICVNTLRANTLLYEALLENRVEELSGYSDVKTEVRYGTENSRVDLLLQAPDKIDCYIEVKSVTLLQHECGYFPDAVTLRGQKHLRELQQMVSNGKRAVLFFAVLHSGIQQVSPARHIDSRYAELFTEAQRAGVEILCYGSTLCPDGITLTHKLPLLG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must have high specificity towards DNA binding. The designed protein sequence is ",
    "response#1": "AGVDWASESHHVFLTDGDGRKIGERVFRHGGEGLAEMAAWLMATSGAVEGDEIQVAIEVPHGPVVETLIERGFKVNAINPKQMDRFRDRFTMAGAKDDSRDAEVMASALRTDPRCFRLLAVSDPVVIELREWSRIAEDLSAERNRLTNRMREQLWRYFK",
    "response#2": "AGVDWASESHHVFLTDGDGRKIGERVFRHGGEGLAEMAAWLMATSGAVEGDEIQVAIEVPHGPVVETLIERGFKVNAINPKQMDRFRDRFTMAGAKDDSRDAEVMASALRTDPRCFRLLAVSDPVVIELREWSRIAEDLSAERNRLTNRMREQLWRYFG",
    "response#3": "AGVDWASESHHVFLTDGDGRKIGERVFRHGGEGLAEMAAWLMATSGAVEGDEIQVAIEVPHGPVVETLIERGFKVNAINPKQMDRFRDRFTMAGAKDDSRDAEVMASALRTDPRCFRLLAVSDPVVIELREWSRIAEDLSAERNRLTNRMREQLWRYFG"
  },
  {
    "reference": "MQRTIKSFFQPKLGAEVKTKEEKVKNDVQKEKEEPEKSVPERPLKERNGRALCGDTESPVKRVSKKSARVLESDSEEEEENSKVQATPEKMNNESDPKNDVKETPPSARKETPPSARKETPPSARKETPQSARKETPPSARKDAKSEGASEMNTSQDFVSPCSSKSIDSPLCSDSPGISPSGIPKRKTARKQLPKRKLEISPSESNPPKDDFDVKGAVKRQKKEAEGDIQQEEPMETSHNISMEEECPIKKETNPDVVQETISNADDPNIELVDQKDVVSGEKQSEPKCQEQPQPKLTSPTVEPKASKGKARKKNSPLPKKVKFSDKVSFKDNDSEEKSNEEKSDEEPQKKADSAKPVKQISSFFAPRKPAIKTEKREENLNEKNVTETSLEESPKPKKNVGSFFGASKQEATEEQTEYNPSKSKYHPIDDACWCNGQKVPYLAVARTFERIEEESARLKNVETLSNFLRSVIALTPEDLLPCIYLCLNRLGPAYEGLELGIGETILMKAVAQATGRQLEKIKAEAQEKGDLGLVAESSRSNQRTMFTPPKLMASGVFGKLKDIARMTGNASMNKKIDIIKGLFVACRHSEARYIARSLGGKLRIGLAEQSVLSSIAQAVCLTPPGRDAPPTVMDAGKGMSADARKSWIEEKAMILKQTFCELPNYDAIIPILLEHGIDDLPKHCRLTPGIPLKPMLAHPTKGIGEVLKRFDEAAFTCEYKYDGERAQIHILENGEVHVYSRNQENNTTKYPDIISRIPKIKKESVKS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be designed to catalyze the following reaction ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-   (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP +   diphosphate..2. The protein design must be able to perform DNA binding in order to contribute to DNA repair, DNA replication, DNA biosynthetic process.3. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.4. The presence of Mg(2+) is necessary for the protein to function in the desired environment.5. For general function, the protein need meet that Also involved in DNA replication and DNA recombination.6. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "MLATPAESANEAFAEFEHAIVEDKYDGIRAQAHISRDKVRIFSRTLDDITDSFPELIPALKAIEHEVILDGEILAWRCGQALAFSELQKRLGRKNVSAAMQREVPVSYVTFDLLYAKGQLVIDRPLQERAAMLDGIFSEGAPRLVNVDPHGQASLMFAEVTPEQRVLRAPQARADSPEELDRLFAAAQERGNEGLMIKDIHSAYAVGRRGKSWLKLKAEADAVSLLVQDAVWTFLPDALVTITGGFRRGKEFGHDVDFLITSPGAEKEQEDQLLQKVTNLWKKQGLPEAIKEYPVNVFLFDLMYYEDVDYTTKPLEVRRKLLESIVKPNDYVKIAHHIQVNNVEDLKSFFYRAISEGGEGVMVKAIGKDAIYQAGARGWLWIKLKRDYQSEMADTVDLVVVGGFYGKGKRGGKISSLLMAAYR",
    "response#2": "MLATPAESANEAFAEFEHAIVEDKYDGIRAQAHISRDKVRIFSRTLDDITDSFPELIPALKAIEHEVILDGEILAWRCGQALAFSELQKRLGRKNVSAAMQREVPVSYVTFDLLYAKGQLVIDRPLQERAAMLDGIFSEGAPRLVNVDPHGQASLMFAEVTPEQRVLRAPQARADSPEELDRLFAAAQERGNEGLMIKDIHSAYAVGRRGKSWLKLRMPREEAAEIEQMVRVSAQAFNPGLLCVACGSFRRGKVTCGDVDVLITHPDGRSHQGIFSPLLDSLRQQGFLTDDLVSQEENGQQQKYLGVCRLPGAGQRHRRLDIIVVPPDFKLFDILYYNQEMLIDLPFRERRNILTQVASRYVTPQLVSDDETEIEAYYHTALDAGHEGVMLKLQGSRYTPGVRGKDWVKIKPEADTLDLVVTGAEWGEGKRAHVFGSFLLSVRK",
    "response#3": "AMQREVPVSYVTFDLLYAKGQLVIDRPLQERAAMLDGIFSEGAPRLVNVDPHGQASLMFAEVTPEQRVLRAPQARADSPEELDRLFAAAQERGNEGLMIKDIHSAYAVGRRGKSWLKLKRELAMLDVVVTAVELGHGKRAGILSDYTFAVRVPLSVLFFDALHTAGEDLIARPASERHLALAAAVPDALRVPRLVTSDGAAAAAFLEGALARGHEGLLAKSLTAPYEAGRRGASWLKVKRAHTLDLVVLAAEWGSGRREGFLSNLHLGARRLTREVPLSLFLFDVLYHRGPLIDEPLWHRREVLESILSEIPGRVEASRMVDVGPDNLDDALWLFKESIREGHEGIMIKDTEAPYIPGIRGKKMLKFKAEPETLDLIVVGGTYGRGKRAHLVGSYLLAVRGG"
  },
  {
    "reference": "MITIVDYGLGNIKAFANLYKKLNIELVFASDVNDLANATKIILPGVGAFDHAMQMLNDSGMRETLDELVLEKKVPVIGICVGMQMMADSSEEGQLKGLGWIPGTVKKFKRESQALESQYPLPHMGWNSLEIARTSELLLGLDEQKLFYFLHSYYYEPNDKEHVVATANYGFEYACIVNKDNVYGIQCHPEKSHHNGVALLKSFASL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that can perform glutaminase activity, lyase activity in cytoplasm to support glutamine metabolic process.2. The designed protein must have a active site active site that is highly conserved among related enzymes.3. For general function, the protein need meet that IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate4. The protein design should consider the endogenous proteins and enzymes present in the Amino-acid biosynthesis, L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9 pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "LSDAYLSVVEALDHSGIAIESEVKLRWINAEDIEEQGPEKLLNDVSGIIVPGGFGIRGVDGKVKAIEYARKQKIPFLGLCLGMQCAVIEWARNIAHLERANSVEFDSETPNPVINLLPEQHDVVDLGGTMRLGLYPCRLTPDTLAIALYQQEVIYERHRHRYEFNNAFRSLLLKTDYLISGTSPDGRLVEIVELTGHPFFIATQFHPEFRSRPNNAHPLFLGFVLSDAYLSVMESLKIAGYEIKKKVKIVWVNARTLTKDNLEKELEGTKGILVPGGFGESGIEGKILAANYARINNVPYLGICLGMQIACIEFARNVLGLENAHTTELNPKTNYPIIDIIEGKDREKIGGTLRLGRYETSLVENTLASKLYNSSTAIERHRHRYEFNNEFKDQFEKAGMVFSGMYDKEDLVEIIEIPSHKFFIASQYHPEFTSRPNKPNPLFMGFVARK",
    "response#2": "LSDAYLSVVEALDHSGIAIESEVKLRWINAEDIEEQGPEKLLNDVSGIIVPGGFGIRGVDGKVKAIEYARKQKIPFLGLCLGMQCAVIEWARNIAHLERANSVEFDSETPNPVINLLPEQHDVVDLGGTMRLGLYPCRLTPDTLAIALYQQEVIYERHRHRYEFNNAFRSLLLKTDYLISGTSPDGRLVEIVELTGHPFFIATQFHPEFRSRPNNAHPLFLGFVKS",
    "response#3": "LSDAYLSVVEALDHSGIAIESEVKLRWINAEDIEEQGPEKLLNDVSGIIVPGGFGIRGVDGKVKAIEYARKQKIPFLGLCLGMQCAVIEWARNIAHLERANSVEFDSETPNPVINLLPEQHDVVDLGGTMRLGLYPCRLTPDTLAIALYQQEVIYERHRHRYEFNNAFRSLLLKTDYLISGTSPDGRLVEIVELTGHPFFIATQFHPEFRSRPNNAHPLFLGFVRD"
  },
  {
    "reference": "MRKIVLATGNAGKAREFAAVLAGFELEIVPQSAFDVPEAEEVGLTFVENALLKARNAALHTGLPALADDSGLVVDALDGRPGIHSARYAGPGADDCANIAKLLVELRGVPAERRTARFVCTLALLRHPADPTPLICQGSWEGLILTEPRGANGFGYDPVFFAPGERRTAAEMDPADKNRLSHRGRALALLRGQLRDGAAR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP2. The protein should have a high affinity for Mg(2+).3. The designed protein should be optimized for ITP diphosphatase activity, XTP diphosphatase activity activity in order to improve nucleotide metabolic process.4. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "MTATERLVLATHNAHKVTELRAILADSVPGVELVGYDGPEPVEDGVTFAENALIKARAAHAATGLPALADDSGICVDVLGGAPGIFSARWAGAHGDDEANLRLLLAQLADVRDEHRGAQFVCAAALVLPDGTEHVVEGRWPGTLARAPAGAGGFGYDPILQPEGTERTCAELSPEEKNRASHRGRAFRALVPVIRELAG",
    "response#2": "MKIILLAAGNSTRLGLKTKKQWLQIKNQTLLEITLKKLSSFCDFIIVTHNNDEYSFLKEKYKNIIFVKGGATRQESTLNGLKHISTEYVCLHDIARPCIKKNLIKKLIEDIKNTDACIPILNISDTIKIKKKNILRETVPRESLYLIQTPQAFKFETILNAHKKFNNKFSDDSAALEYMGKKCILIEGDISNYKITTPKDLNYLEKIIG",
    "response#3": "MEIILATRNKGKIREIKDMFA"
  },
  {
    "reference": "MLLTINNKIFTNNSKEANKDTIFVCNELNRKFIDDARANGCVEFVEDYELGNFFDFSQIKIVGVTGTNGKTTTTAAIYSILLDLGYKVALQGTRGFFINDEKMEGYSLTTPMQLDIYAHIKKALELGCEFFVMEVSSHAIAQNRIAGLNFALKVHTNITRDHLDYHKTIEEYINVKNSFFTDESMKLINKDDKHIKYNIKNCFAYGLENPSTYKVSAYSLQNGIHVMLDNFENKVAFSSSMMGIFNIYNLTAAVASVHLLTKMDLSEICDQVENFGGVSGRMEIASTEPLIIIDFAHTPDGMEEVFKSFANKDIIAVFGAGGNRDKEKRPLMGKIASRYCKHIIITSDNPRFEDPDLIVNDILLGISNKTNIIVELNRKEAIKKAIQLYKKDTVILILGKGDEEYQIVYDHKIPFKDYNIVKELL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.2. The protein design should aim to mimic or enhance the function of natural proteins involved in cell division, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "ITGTNGKTTTAFIAHAILKNVQHRAGLLGTIKVDNGLEEREATHTTPDPLQLQQVFSEMVENGCRAVAMEVSSHALHQKRTAGTSFDVGVFTNLSQDHLDYHVSMEAYFNAKKLMFESMVASESKKRPTAVINIDERHGELLVRDYKDDLRILTFGFSVHADFRAVNVKQGMSGTQFELQYKGKDYLVKTPYFGLYNVSNALAALR",
    "response#2": "ITGTSGKTTTSYLVEAALLAAGHSVGLIGTVETRVNGIAQPSSLTTPEAPTLQALLAAMLEDGVDIVVMEVSSHALALGRVDGAHFAIGAFTNLSQDHLDFHQTMRAYFDAKAQLFVPSAPTHAVRAVVCVDDEWGRRMAELARRPGEAAHLQPVLVSTGPTPAHWHAGESSLDADGSSRTPFAEPDGEHELIIPLPGRYNVANGLLAVAVAEVLGVDRASFVSALPAFAGVEGRFEIVRAANGTTAVVDYAHTPDALENVLRTIEEIRTPQQQLLVVCGCGGDRDRTKRPEMAQIAVQYASTAIFTSDNPRHESPEAILDEMVAGLDPGTRYLRIADRAEAIRTAVMLSHPGDVILVAGKGHETGQIVGDRVLPFSDHAELRAAL",
    "response#3": "VTGTSGKTSVAAFTRQIWEQAGYAAASIGTTGVVAPNRNEYGSLTTPDPVALHRLLKELADAGVTHASMEASSHGLDQRRLDGVRLAAGGFTNLGRDHMDYHPTVEEYHRAKLRLFDTLLPKGAPAVIFADDPWSGPTIKAAQAAGLDVLTVGRHGSFLKLKRVEHERHRQRAEITVDGTLYEIDLPLAGDFQISNALVSAGVTGTSGKTSVAAFTRQIWEQAGYAAASIGTTGVVAPNRNEYGSLTTPDPVALHRLLKELADAGVTHASMEASSHGLDQRRLDGVRLAAGGFTNLGRDHMDYHPTVEEYHRAKLRLFDTLLPKGAPAVIFADDPWSGPTIKAAQAAGLDVLTVGRHGSFLKLKRVEHERHRQRAEITVDGTLYEIDLPLAGDFQISNALVSAGLR"
  },
  {
    "reference": "MATRLLLFLLVSVTFSHAQFHSGQRQKPRLQRDRRNIRPNIILILTDDQDLELGSMQVMNKTRRIMEEGGTFFSNAFVTTPMCCPSRSSMLTGKYVHNHNTYTNNENCSSLSWQRHHEPRTFGVYLNGTGYRTAFFGKYLNEYNGSYVPVGWREWLGLVKNSRFYNYTLSRNGVREKHGAQYPEDYLTDLITAESVRYFRSSKRVYPHRPILMVLSHAAPHGPEDSAPQYSTAFPNASQHITPSYNYAPNPDKHWIMRYTGPMKPIHMEFTNLLQRKRLQTLLSVDDSMEKLYNMLVETGELENTYIIYTSDHGYHIGQFGLVKGKSMPYEFDIRVPFYIRGPNVEQGSINPHIVLNIDLAPTILDIAGLDTPPDMDGKSILTLLDTDKPANRFQVSRKSKVWRDSFLVERGKLLHQRVEGKEVSLEENFLPKHQRVKDLCQRAEYQTPCQQTGQKWQCVEDATGKLRLFKCKNEGGGAKNLQRGVASRQRPISIKSQLYLLDSDACSCDFSDLRHVNFGPFVSKSFNKKSFFKKKFKTLKKYSKNRFSRSLTELYDHHGNRSLVGGASPGQPMRDESEDESSGMGGVSNTAAPSSAIKVTHRCSILSNATVKCDTGVYRSLQAWKDHKLHIDHEIETLQTRINNLREVRGHLKKSRPLQCDCNKYLYKAKMKKKLRYRGGGGLHPFRKGGARDEKAWLQKEQRRRKKMRKMIKRIKNNDTCSMPGLTCFTHDNQHWHTPPFWTLGSFCACTSANNNTYWCLRTINDT",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should contain one or more Sulfatase N-terminal domains that are essential for its function.2. The protein should be optimized for stability and solubility when expressed in endoplasmic reticulum where it will be involved in heparan sulfate proteoglycan metabolic process.3. Ca(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "PQAIIIGVKKGGTRALLEFLRVHPDVRAVGAEPHFFDRSYDKGLAWYRDLMPRTLDGQITMEKTPSYFVTREAPARISAMSKDTKLIVVVRDPVTRAISDYTQTLSKRPDIPTFESLTFKNRTAGLIDTSWSAIQIGIYAKHLEHWLRHFPIRQMLFVSGERLISDPAGELGRVQDFLGLKRIITDKHFYFNKTKGFPCLKKAEGSSRPHCLGKTKGRTHPEIDREVVRRLREFYG",
    "response#2": "KPRFIHTRPIRSISVELEGEIYDIDLEIKELKSLMPKNTSTLYQEENPAEEGDEDNEMIAEDNSKADESVLDSVKVTHKCFILANDTVHCEKELYQSARAWKDHKSYIDKEIEALQDKIKNLRDVRGHLKRKKPEECDCNC",
    "response#3": "KSYARNRYSRSVEMEIGGDLYAVDLEDGYQPVPAPRNLSQAGARTPRDDSEDDFSGMGVTVTVTPRTSNNSLAIVSSGIKVTHRCSILANDTVKCDTGIYKSLQAWKDHKLHIDHEIETLQTKIKNLREVRGHLKKARPEECDCS"
  },
  {
    "reference": "MAELTISSDEIRSAIENYTASYSPEASREEVGVVTDTSDGIAHVSGLPSAMANELLEFPGGILGVALNLDATEIGAVILGDYENIQEGQEVKRTGDVLSVPVGDAFLGRVINPLGQPIDGLGEIASDETRALELQAASVLERQPVEEPLQTGIKAIDAMTPIGRGQRQLVIGDRKTGKTAVCIDAILNQKANWETGDEKQQVRCIYVAIGQKGSTIAGVKAALEEQGAMEYTTIVAAPASDSAGFKWLAPYTGSAIGQHWMYQGKHVLVVFDDLTKQAEAYRAISLLLRRPPGREAYPGDVFYLHSRLLERSAKLSDALGGGSLTALPIIETKANDVSAYIPTNVISITDGQVFLESDLFNKGVRPAINVGISVSRVGGAAQTKGMKKVSGSLRLELAQFRELEAFSAFASDLDAASKAQLERGARLVELLKQDQYSPIPVEDQIVSIYLAGEGVFDSVPVGDVRRFEAELLDELHRTASGVYESIKGGKALDADNAKALVEATDKFKETFLASDGSRVVNEAEAEALDAGEVGHEQINVKRTTVSK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must be able to ATP binding. The designed protein sequence is ",
    "response#1": "LDYFFGKDFSQNLIEFTEENDFMKLRGYLGKPAMMKRTKNQQFLFINNRVTQSKLLSSAIMTAYGELLGEREYPFFLIYMDIAPHYIDVNVHPTKMEVKFDDERNIYNMVHSVVKHRIR",
    "response#2": "FFGKDFSQNLIEFTEENDFMKLRGYLGKPAMMKRTKNQQFLFINNRVTQSKLLSSAIMTAYGELLGEREYPFFLIYMDIAPHYIDVNVHPTKMEVKFDDERNIYNMVHSVVKHRR",
    "response#3": "LTERLCVRPTSETLFCEHYSNSIQSYRDLPKLYNQWCSVVRWEKTTRPFLRTLEFLWQEGHTAHATAEEAQEETIRMLNVYADVLENVLAIPVIKGRKTEKEKFAGAHATYTVESLMHDGKALQSGTSHNFGDGFAKAFDIQYTDKNNQLQYVHQTSWGVTTRLIGAIIR"
  },
  {
    "reference": "MVDWASDSDNDKFEWDTDGEAETSSAPALRNIDAPGPSTRLPQDANGKANGSGALVAEFMGMGFPKEMILKAIKEIGDTDTEQLLELLLTYQAIGGDASVGNCSASACAPQTLEVDEEEDDTNWDEYDTAGNCDRTPHSDGSGDEDFFQEMSEKDEKMKSLVNMGFPEDEAKMAIDRCGLDAPVAVLVDSIYASQEAGNGYSANLSDYEDTEFSSFGGRKKTRFVDGSKKRKRYGSGPSGNQVPFDGSHEEPMPLPNPMVGFSLPNERLRSVHRNLPDQALGPPFFYYENVALAPKGVWTTISRFLYDIQPEFVDSKYFCAAARKRGYIHNLPIENRSPVLPMPPKTISEAFPNTKRWWPSWDPRRQFNCLQTCMASAKLTERIRCALGRFSDVPTPQVQKYVLDECRKWNLVWVGKNKVAPLEPDEMEFLLGYPRNHTRGVSRTERYRALGNSFQVDTVAYHLSVLRDLFPNGMNVLSLFSGIGGAEVALHRLGIHMKTVISVEKSEVNRTILKSWWDQTQTGTLIEIADVRHLTTERIETFIRRFGGFDLVIGGSPCNNLAGSNRHHRDGLEGEHSALFYDYIRILEHVKATMSAV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Required for normal establishment and maintenance of RNA-directed DNA methylation (RdDM) mediated by small interfering RNAs (siRNAs); Regulates proper plant development in both vegetative and reproductive stages through DNA methylation..2. The designed protein should be optimized for DNA binding, DNA (cytosine-5-)-methyltransferase activity activity in order to improve DNA methylation.3. The protein contains novel UBA domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "GLISHQLNSYNYFIEHGLQNVFQSFGEMLVEPSFDVVKKKDNDWRYATVKFGEVTVEKPTFFSDDKELEFLPWHARLQNMTYSARIKVNVQVEVFKNTVVKSDKFKTGQDNYVEKKILDVKKQDILIGSIPVMVKSILCKTSEKGKENCKKGDCAFDQGGYFVIKGAEKVFIAQEQMCTKRLWISNSPWTVSFRSENKRNRFIVRLSENEKAEDYKRREKVLTVYFLSTEIPVWLLFFALGVSSDKEAMDLIAFDGDDASITNSLIASIHVADAVCEAFRCGNNALTYVEQQIKSTKFPPAESVDECLHLYLFPGLQSLKKKARFLGYMVKCLLNSYAGKRKCENRDSFRNKRIELAGELLEREIRVHLAHARRKMTRAMQKHLSGDGCHVCGGKQDPDKQLMCDECDMAFHIYCLRPPLSSVPPEEEWYCPDCSTSCIEDCLASEMSCNCAIGVDNGFAYTLDGLLKEEFLEARISEARDQRKQVLRFCEECPLERAKKVEILEPCKGHLKRGAIKECWFKCGCTKRCGNRVVQRVRLQVFKSEKKGWGVRCLDDIDKGTFVCIYSGRLLRRATPEKTNIGENGREQQHIVKNSFSKKRKLEVVCSDCDAHCDSPKAEDCPPKLSGDLKEPAVEMNHRNISRTQHHSVIRRTKSKTTVFHYSEKNMGFVCSDSAAPEDKNGFKPAQEHVNSEARRAHEDLSSNPAGDSEDTQLTESDVIDITASREDSAPAYRCKHATIVDRKDTKQVLEVPGKKSQEEEPAASQSQQALCDEELPSERTKIPSASLMQLSKESLFLLDASKEGNVGRFLNHSCCPNLWVQNVFVETHDRNFPLVAFFTNRYVKARTELTWDYGYEAGA",
    "response#2": "MKDITAGEENVEIPWVNEINEKVPSRFRYMPHSFVFQDAPVIFSLSSFSDEQSCSTSCIEDCLASEMSCNCAIGVDNGFAYTLDGLLKEEFLEARISEARDQRKQVLRFCEECPLERAKKVEILEPCKGHLKRGAIKECWFKCGPRGWEMKALSDLGQIICGKTPSKSNKEFYGDDVPFIKIPDMHNQVFITQTTDNLSVVGANYQSKKYIPAKSICVSCIATVGLVSMTSKPSHTNQQINSIIPDDEQSCEFLYLSLKQPSMTKYLKDLASGGTATLNLNTSTFSKIEIITPSKEIIYIFQKKVVSIFEKTLSNSIENKRLTEGWGVRCLDDIDKGTFVCIYSGRLLRRATPEKTNIGENGREQQHIVKNSFSKKRKLEVVCSDCDAHCDSPKAEDCPPKLSGDLKEPAVEMNHRNISRTQHHSVIRRTKSKTTVFHYSEKNMGFVCSDSAAPEDKNGFKPAQEHVNSEARRAHEDLSSNPAGDSEDTQLTESDVIDITASREDSAPAYRCKHATIVDRKDTKQVLEVPGKKSQEEEPAASQSQQALCDEELPSERTKIPSASLMQLSKESLFLLDASKEGNVGRFLNHSCCPNLWVQNVFVETHDRNFPLVAFFTNRYVKARTELTWDYF",
    "response#3": "GLISHQLNSYNYFIEHGLQNVFQSFGEMLVEPSFDVVKKKDNDWRYATVKFGEVTVEKPTFFSDDKELEFLPWHARLQNMTYSARIKVNVQVEVFKNTVVKSDKFKTGQDNYVEKKILDVKKQDILIGSIPVMVKSILCKTSEKGKENCKKGDCAFDQGGYFVIKGAEKVFIAQEQMCTKRLWISNSPWTVSFRSENKRNRFIVRLSENEKAEDYKRREKVLTVYFLSTEIPVWLLFFALGVSSDKEAMDLIAFDGDDASITNSLIASIHVADAVCEAFRCGNNALTYVEQQIKSTKFPPAESVDECLHLYLFPGLQSLKKKARFLGYMVKCLLNSYAGKRKCENRDSFRNKRIELAGELLEREIRVHLAHARRKMTRAMQKHLSGDGGLISHQLNSYNYFIEHGLQNVFQSFGEMLVEPSFDVVKKKDNDWRYATVKFGEVTVEKPTFFSDDKELEFLPWHARLQNMTYSARIKVNVQVEVFKNTVVKSDKFKTGQDNYVEKKILDVKKQDILIGSIPVMVKSILCKTSEKGKENCKKGDCAFDQGGYFVIKGAEKVFIAQEQMCTKRLWISNSPWTVSFRSENKRNRFIVRLSENEKAEDYKRREKVLTVYFLSTEIPVWLLFFALGVSSDKEAMDLIAFDGDDASITNSLIASIHVADAVCEAFRCGNNALTYVEQQIKSTKFPPAESVDECLHLYLFPGLQSLKKKARFLGYMVKCLLNSYAGKRKCENRDSFRNKRIELAGELLEREIRVHLAHARRKMTRAMQKHLSGDG"
  },
  {
    "reference": "MENFKLASIEDALKDFKEGKFVIVVDDEDRENEGDLIIAAEKITPEKVNFMLKHARGVLCAPITLSRCEELDLPHQVADNTSVLGTPFTVTVDKLEGCTTGVSAHDRAATILALADPTSTPQTFGRPGHINPLYAQDNGVLRRCGHTEATVDLCRLAGLYPAGALMEIMNEDGSMARMPELQKLATEWGLKIITIADLIAYRLEKESIIEVGDEVDMPTRYGHFHLIPFRQQSNGLEHMALVKGKWERDEPVLVRVHSSCATGDILGSLRCDCGQQLQKSMELIEKEGKGVVIYMQQEGRGIGLMNKVAAYKLQEEGYDTVDANIHLGFKPDERDYGCGAQMLRHLGVRKMRLLTNNPVKRVGLEAYGLEIAEIVPIEVMPNRYNERYLKTKQVRMGHKLHLK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein have GTP cyclohydrolase II domains with specific sequence motifs that are crucial for its activity.2. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..3. A protein should be able to manipulate the activity of enzymes in the Cofactor biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway, either by activating or inhibiting their function.4. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation.5. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile; for GTP cyclohydrolase activity, Proton acceptor; for GTP cyclohydrolase activity to enable effective catalysis of GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.6. The protein should be able to bind D-ribulose 5-phosphate, Mg(2+), GTP, Zn(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "VKVNMPTKYGDFKLIPFRQISNGMEHVALIKGEWAKDEPVLVRVHSSCITGDIFGSMRCECGDQLHNAMQLIEKEGKGVVVYMNQEGRGIGLMNKIKAYKLQEEGLDTVDANIHLGFQADERDYGVGAQILREVGVCKIRLISNNPVKRIGLEAYGLSIVENVPVTAVPSDRVSDINLPTDFGEFRAIAYTNDMDNNVHIALVKGNIRPEDAVLVRVHSECLTGDVFGSKRCDCGEQLHRAMERIDKERKGVILYMRQEGRGIGLANKLKAYELQDRGLDTVEANLKLGFSPDLRDYGIGAQILVDLGVRKMRLMTNNPKKIVGLEGYGLKVLERVREVSIDLPTQFGDFKAYAYSNVVDGKEHIALVKGEVAPEKPTLVRVHSECLTGDVFGSQRCDCGPQLHAALEQIEEAGQGVLLYMRQEGRGIGLLNKMRAYKLQEEGYDTVEANEKLGFAPDLRDYGIGAQILRDVGVKKMKLLTNNPRKIRGLEGYDLEVVDRVREVVTDLPTHYGNFKLIAYRTLLEDRIHLALIKGKVRNKEDVLVRVHSQCLTGDVFRSLRCDCGDQLEIALYMIGKASHGVVLYLNQEGRGIGLLNKLKTYVLQDRGMDTVEANQKLGFEPDLRSYGIGAQILADLGLHRIRLLTNNPRKIVGLEGYGLEISERTYPQVEMSKEAEAQLPTRFGDFKIKVYKSIIGNQEHIALVKGEVAGKKNVLVRVHSECLTGDTFGSLRCDCGQQLAAALKMIDKEGHGVLLYMRQEGRGIGLLNKIKAYALQDKGMDTVEANLALGFPPDLRDYGIGAQILADLGLTSIRLLTNNPRKIIGLEGYGLMVVDRV",
    "response#2": "MSRLATTKLPTKSGGEFTAIAFGNFVDSSVHIALVKGEIKPTDEVLVRVHSECLTGDVFGSRKCDCGEQLHKAMEIIKGEGKGVILYLRQEGRGIGLANKLKAYELQDKGLDTVEADIKLGFTPDLRDYGIGAQILVNLGVRKMKLMTNNPRKIVGLEGYGLKVVKRVGGGVFGDERVAEARLPSRYGEFKIVAYKSYIDPGEHIALTLGDCNSADAVLTRVHSECLTGDVFGSMRCDCGEQIDLALRMIGKEGTGILLYMRQEGRGIGLHNKIKAYSLQEGGLDTIEANEQLGFESDLRNYALSAQMLLDLGISKVKLVTNNPKKVVGLSGYGVEITDRQEIDMPTDFGDFRMFLYRAEIDNHHHLALVKGPINPKKPALVRVHSECLTGDVFGSRRCDCGSQLHSALAQISESGHGVLVYMRQEGRGIGLPAKIHAYKLQEEGLDTVEANERLGYPPDLRDYGTGAQILADLGVTKIRLLTNNPRKVVGLEGYGMEIIERVPLQYPQERVAEARLPSRYGEFKIVAYKSYIDPGEHIALTLGDCNSADAVLTRVHSECLTGDVFGSMRCDCGEQIDLALRMIGKEGTGILLYMRQEGRGIGLHNKIKAYSLQEGGLDTIEANEQLGFESDLRNYALSAQMLLDLGISKVKLVTNNPKKVVGLSGYGVEITDRRVAETKLPTQYGEFKAVAYASIIDSDEHVVLVKGDISTNDPVLVRVHSQCLTGDTFGSQRCDCGEQIHRSLEAIQAEGRGVFLYMRQEGRGIGLHNKLRAYQLQDAGLDTVEANEKLGFPADLRWYGIGAQILVDLGIKRIRLLTNNPKKVVGLDGYGLEIMERV",
    "response#3": "MSREVSIDLPTQFGDFKAYAYSNVVDGKEHIALVKGEVAPEKPTLVRVHSECLTGDVFGSQRCDCGPQLHAALEQIEEAGQGVLLYMRQEGRGIGLLNKMRAYKLQEEGYDTVEANEKLGFAPDLRDYGIGAQILRDVGVKKMKLLTNNPRKIRGLEGYDLEVVDRVPLMPMSEVDMPTDIGHFRLRLYHSSVDGLDHLALVKGAPGDAAEPPLVRVHSECMTGDVFHSARCDCGRQLEAAMRMIEQAGCGVVLYMRQEGRGIGLANKLHAYVLQEKGLDTVDANEQLGFSADLRDYGVGAQILADLGLQRIRLLTNNPRKVVGLQGYGLEIVERVPLMPM"
  },
  {
    "reference": "MALKIRLARGGSKKRPYYQIVVADARSPRDGRFLEKLGSWNPMLAKDDEKRIELNAERVQHWIAQGAQPTDRVMRFLDQAGLAKRPARNNPTKAQPGKKAQERAAEAKQKAEEAAAAASEAAAE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a specific function within the ribosome. The designed protein sequence is ",
    "response#1": "EIDVHVPIHLVGEAAGVKDGGVLQQAMHQLSIRVLPSNIPPSIDVDITNLQVGDVITVSHIQTNGAYEINHDPTEVIASILPPQ",
    "response#2": "EIDVHVPIHLVGEAAGVKDGGVLQQAMHQLSIRVLPSNIPPSIDVDITNLQVGDVITVSHIQTNGAYEINHDPTEVIASILPPL",
    "response#3": "EIDVHVPIHLVGEAAGVKDGGVLQQAMHQLSIRVLPSNIPPSIDVDITNLQVGDVITVSHIQTNGAYEINHDPTEVIASILPPS"
  },
  {
    "reference": "MIELDFEKTGGLLPAICQDAETGEVLMLAFMNKESWEKTLETGMATYWSRSRQELWTKGLTSGNVQKVKEIRVDCDDDTILLKVEQIGGAACHTGHRSCFHKLVEGDKLTVVGEPVFDPKEVYKK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that localizes to cytoplasm and can perform zinc ion binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "SRNYSDSPLTDLENGFKLITRHQKRRYIDIEDRINFQNLTDFRLGHIKNCATWLTHSSFFQSLTETENLLILKSTWHVWSWLELLSVSVEIFGNQVCEEKIVFLSEKIAVDIVKVFRYILKPLNKQEKRKVEKELNPIFHILFDDVARKLQNLKPSSLEINYMLWQLVWFVAEKVLNEDNLRHGEQYTNQLASDLHNHYKNDLHLEQYAQRVLKMMAIVKSLQKHLMNIHKIIDYADNFCNLFAMF",
    "response#2": "ILFLTDLHWDHDYLEGTDPDCADPLCCRRGSGLPPASRPGAGYWGEYSKCDLPLRTLESLLSGVGLAGPFDMVYWTGDIPAHDVWQQSRQDQLRALNTITALVRKFLGPVPVYPAVGNHESTPVNSFPPPFIEGNHSSRWLYEAMAKAWEPWLPAEALRTLRIGGFYALSPRPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVGELQAAEDRGDKVHIIGHIPPGHCLKSWSWNYYRIVARYESTLAGQFFGHTHVDEFEVFYDEETLSRPLAVAFLAPSATTYIGLNPGYP",
    "response#3": "ILFLTDLHWDHDYLEGTDPDCADPLCCRRGSGLPPASRPGAGYWGEYSKCDLPLRTLESLLSGVGLAGPFDMVYWTGDIPAHDVWQQSRQDQLRALNTITALVRKFLGPVPVYPAVGNHESTPVNSFPPPFIEGNHSSRWLYEAMAKAWEPWLPAEALRTLRIGGFYALSPRPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVGELQAAEDRGDKVHIIGHIPPGHCLKSWSWNYYRIVARYESTLAGQFFGHTHVDEFEVFYDEETLSRPLAVAFLAPSATTYIGLNPGYG"
  },
  {
    "reference": "MGGSRRRGSRHKARVRAVEILYEAEVRGVPVSEVIERRRAQTEPPINEFTEQLATRVDEHRARIDELLETYAIGWTLDRMPVVDRNILRIGVYELLWADDIPDGVAIAEAVAMAKELSTDESPVFVNGLLSRLMEKKPSLSL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Involved in transcription antitermination2. The designed protein should have RNA binding to facilitate DNA-templated transcription termination, regulation of DNA-templated transcription, transcription antitermination. The designed protein sequence is ",
    "response#1": "EIREHLFRMLFRKEFHEPTELEEQVLFYFDSLESITPEQQEYLNVRFDKINEKLGEIDTILANASSGWKLNRMGKVDLNIMRLATFEIRFDDEVPVKVAINEAIELAKKYGGDSSASFVNGILAKVR",
    "response#2": "PGEWYVIHTYAGYENRVKTNLEQRAVSLNVEDYIFQAEVPQEEVVQIKNGDRKTIRQNKLPGYVLVRMDLTNESWGVVRNTPGVTGFVGNAYDPYPLTLDEIVKMLAPQ",
    "response#3": "EIREHLFRMLFRKEFHEPTELEEQVLFYFDSLESITPEQQEYLNVRFDKINEKLGEIDTILANASSGWKLNRMGKVDLNIMRLATFEIRFDDEVPVKVAINEAIELAKKYGGDSSASFVNGILAKVR"
  },
  {
    "reference": "MPAKLNSPSRYHGIYNAPHRAFLRSVGLTDEEIGKPLVAIATAWSEAGPCNFHTLALARVAKEGTKEAGLSPLAFPTMVVNDNIGMGSEGMRYSLVSRDLIADMVEAQFNAHAFDGLVGIGGCDKTTPGILMAMARLNVPSIYIYGGSAEPGYFMGKRLTIEDVHEAIGAYLAKRITENELYEIEKRAHPTLGTCSGLFTANTMGSMSEALGMALPGSASPTATSSRRVMYVKETGKALGSLIENGIKSREILTFEAFENAITTLMAMGGSTNAVLHLLAIAYEAGVKLTLDDFNRISKRTPYIASMKPGGDYVMADLDEVGGVPVVLKKLLDAGLLHGDVLTVTGKTMKQNLEQYKYPNVPHSHIVRDVKNPIKPRGGIVILKGSLAPEGAVIKVAATNVVKFEGKAKVYNSEDDAFKGVQSGEVSEGEVVIIRYEGPKGAPGMPEMLRVTAAIMGAGLNNVALVTDGRFSGATRGPMVGHVAPEAMVGGPIAIVEDGDTIVIDVESERLDLKLSEEEIKNRLKRWSPPSPRYKSGLLAKYASLVSQASMGAVTRPA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of (2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-   oxopentanoate + H2O.2. The designed protein should interact with enzymes and substrates within the L-valine from pyruvate: step 3/4, Amino-acid biosynthesis pathway to enhance or regulate its activity.3. The designed protein must possess dihydroxy-acid dehydratase activity, magnesium ion binding, 2 iron, 2 sulfur cluster binding to support isoleucine biosynthetic process, branched-chain amino acid biosynthetic process under certain conditions.4. The [2Fe-2S] cluster binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "RIDNPYSETGGLSVLRGNLAPDGAIVKKAAVAPEMMQHTGPARVFNSEEEVSKAILGGKINPGDVVVIRYEGPKGGPGMKEMLSPTASLAGMGLDKSVALITDGRFSGATRGASIGHVSPEAAEGGPIGLVEEGDTIEIDIEKKTINLLVPEEKLKNRKPEKVEKPVKGYLNTYRQGVSSACTGGVTG",
    "response#2": "KPIVAIANSFTQFVPGHVHLKDMGGLVADAVFEAGGIGREFNTIAVDDGIAMGHGGMLYSLPSRELIADAVEYMVNAHCADALVCISNCDKITPGMLIAALRLNIPTVFVSGGPMEAGKTIAIEGVVHEKLDLVDAMSASANENVTDEQLDTIERSACPTCGSCSGMFTANSMNCLTEAIGLALPGNGSTLATHAARKALFERAGELIVDIAKQYYQNDDESVLPRSIANRDAFENAVALDVAMGGSTNTVLHLLAAAREAEIDFSVADIDAVSRRVPCLSKVAPNSPKYHMEDVHRAGGIPALLGELHRGGALRPNVRSVHSPDVIYPIETPITPTGGVVGLRGNLAPDGAIVKVAGMKPEEQIFTGPALVFECEEDAFEAVTHRQYSVGDVIVIRNEGPAGGPGMREMLSTTAALSGQGMGKKVALITDGRFSGATRGFCVGHVGPEAAHGGPIAMLKTGDMITINAVTGEISVDLTDEELAARKADWAGPRQTEYESGALWKYARLVGTARLGKANAGQVLRIDNPYSETGGLSVLRGNLAPDGAIVKKAAVAPEMMQHTGPARVFNSEEEVSKAILGGKINPGDVVVIRYEGPKGGPGMKEMLSPTASLAGMGLDKSVALITDGRFSGATRGASIGHVSPEAAEGGPIGLVEEGDTIEIDIEKKTINLLVPEEKLKNRKPEKVEKPVKGYLNTYRQGVSSACTGAVL",
    "response#3": "MEQPLVGVATCWNEAAPCNIALGRQAQAVKMGVKAASGTPREFTTITVTDGIAMGHEGMRSSLASREAIADTVELTMRGHCYDAIVGLAGCDKSLPGMMMAMVRLNVPSVFLYGGSILPGRFEGRDVTVQDVFEAVGRHQAGLNSAEDLEKLEKVACPSAGACGGQFTANTMACVAEAIGLALPLSSGAPAPYESRDALGEASGQAVMALLESGIRARDVVTRQSLENAARIVACTGGSTNAGLHLPAIAHEAGIAFTLDDVCEIFRDTPYFVDLKPGGRYVAKDLYEVGGVPVVMKELDRAGLIHRDCVTATGRSMGEASLGRADVIRSLERPIHKTGGLTILKGSLAPDGAVVKSAGFDETVFRGTARVFDGERDAMDALAEGRIVAGDVVVIRYEGPKGGPGMREMLAITGAIKGAGLGKDVLLLTDGRFSGGTTGLCVGHVAPEAVDGGPIAFVRDGDPITLDVLNRTLEVEIDDLEARKEGWAPKPPKYTRGVLGKYAKIVSSAAHGDVIRPMEKPYSPQGGIAILYGSLAPEGAVVKQSAVAPEMMQRTARARVFESEEAAFEAIMAQRIEKGDAIIIRYEGPKGGPGMREMLSPTAAIAGIGLGADVALITDGRFSGGTRGAAIGHVSPEAADGGPIGLVREGDMIEIDIPARKLDLLVDEAEMERRRAAFTPVVKEVTSPLLKRYSRLVTSAANGAVLLD"
  },
  {
    "reference": "MQTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFIRTKMTRRNNFTTGRIYADVLRKERRGDYLGATIQVIPHITNEIKERVIKGAEGHDVAIVEIGGTVGDIESQPFLEAIRQMGTEVGRDRAMFMHLTLVPYLGASGEVKTKPTQHSVKELRSIGIQPDILVCRTDRAALPASERSKIALFTNVEEKAVISLKDVDSIYKIPALLKSQGLDDLVCKRFGFNAPEADLSEWEQVIYQEANSQGEVTIAMVGKYVELPDAYKSVNEALKHAGLKNRVSVHIKYIDSQDIETKGTSLLENVDGILVPGGFGERGVEGKVLAAQYARENKVPYLGICLGMQVALIEYARNVAGLKDAHSTEFKKDAEHPVVGLITEWVDEEGGVETRTEASDLGGTMRLGSQLCHLIPGTKAHDLYGSDTIFERHRHRYEVNNKYRPALEKAGLAFTGLSADKKLVEIIEIPSHPWFVAGQFHPEFTSTPRDGHPLFGGFVKAAWEGHKQKA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The protein should have a high affinity for L-glutamine, CTP, Mg(2+), ATP.3. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.4. The protein should be optimized for activity in CTP from UDP: step 2/2, Pyrimidine metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. A protein that contributes to glutamine metabolic process, 'de novo' CTP biosynthetic process by functioning in membrane with CTP synthase activity, metal ion binding, glutaminase activity, ATP binding.6. The Nucleophile; for glutamine hydrolysis, active site, Nucleophile of the protein must be engineered to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+). The designed protein sequence is ",
    "response#1": "MEARGLRVTNIKLDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTLARMTRQNNFTTGRIYEAVISKERRGEYLGATVQVIPHVTDEIKLRIQSAAEGADVAIVEIGGTVGDIESLPFIEAIRQLKIESGSQNALSVHVTLVPFIATAGEMKTKPTQHAVKEMRAIGIQPDVLLCRCDRALPRDMKEKIALFSNVPVDRVVAAMDVACIYELPLALHAEGIDEKLTAPVAALATVDDKPLQDAYLSIVESLNHAGFEELTEIEIDWIYAKDVTDKNVDEILGGLDGIVVPGGFGDQGIEGKLETLRYARETGKPVLGISLGMQLMAIEAARNILGMKDANSTEIDPTTHDPIFTLMSEDAELLDREHSLRIGSYDSVVVEDTLASKLYGVKDISERHRHRFEFNNEYEENIKDAGLTFSGMSEDGKYKEIIEITEHPFYIGAVFHPEFKSRPTRPHPLFKGLVRLKDAYFSVREALHHAGLFHNRDIHLTWIASEELEEKGGQALLRSAQGIIVPGGFGIRGIEGMIEAAKYARNNKIPYLGLCLGMQVMVIEFARHVLGTSEANSTEFEAETPYPVIDLLNDQENVEDMGGTMRLGNYPCDLLRDSHAGRAYGTTRIEERHRHRYEFNNKFRSQLQEAGMVCSGLSPDGKLVETCEIPNHPWMVSCQFHPEFGSRPGQPHPLFRDFLAAAA",
    "response#2": "MEARGLRVTNIKLDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTLARMTRQNNFTTGRIYEAVISKERRGEYLGATVQVIPHVTDEIKLRIQSAAEGADVAIVEIGGTVGDIESLPFIEAIRQLKIESGSQNALSVHVTLVPFIATAGEMKTKPTQHAVKEMRAIGIQPDVLLCRCDRALPRDMKEKIALFSNVPVDRVVAAMDVACIYELPLALHAEGIDEKLTVPLSDIAPPADASGKLPDAYKSINEAFVHAGAKNECKVRLKHIHSEGLTPENAAEKLAGMHGVLVAPGFGSRGIEGKIEAIRYVRENNIPFFGICLGMQCAVIEYARNVIGWKDAHSTEMDDHTKHPVISMMEEQKKIKNKGGTMRLGAYACDLKKGSKAASIYGKAHISERHRHRYEFNSKYLKEFEDAGLIASGINPENNLVEIVELKNHPFFIGGQFHPELKSTVANPHPLFVNFVDSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASFVNAACALARVEIAVVGKYIELHDAYKSIYEALSHGGAAHRHRLRLRKISAEDLAARGPDGFLRGVHGVLIPGGFGERGLEGKIAAAGWCRTRGLPCFGICFGMQAMTIEYARNVVGLAGANSTEVAPDCPHPVIDLMRDQRGVSDKGGSMRLGAYPCRLLDGSVASTIYGRDMVLERHRHRWELNNNYRRRLEQAGLRCSGVYEARDLVEIIELPAHPFWLGVQYHPEFKSKPLQPHPLFAAF",
    "response#3": "MEARGLRVTNIKLDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTLARMTRQNNFTTGRIYEAVISKERRGEYLGATVQVIPHVTDEIKLRIQSAAEGADVAIVEIGGTVGDIESLPFIEAIRQLKIESGSQNALSVHVTLVPFIATAGEMKTKPTQHAVKEMRAIGIQPDVLLCRCDRALPRDMKEKIALFSNVPVDRVVAAMDVACIYELPLALHAEGIDEKLTVPIRSDDLGPWQLIDSYKSVMEAFVHAGVENKARVNIKWVQAEKIKNEKSIKKLFSNINGVLILPGFGARGSEGKIRIAKFSRENKIPFLGICLGLQCAVIEFARNVCNMEGADSTEFNKRTKFPVIDLMESQRAIKIKGGTMRLGAYDCEIKTGTKTYAAYRKKRITERHRHRYEVNNRYKKKLEKNGLIFSGVNKDLGVVEAIEIKGHPWYIAGQFHPELKSRVNKAHPLFREFVEILQ"
  },
  {
    "reference": "MSIKGKNILLGVSGGIGAYKACELLRLFVKAEARVRVIMTEAAQEFVTPLTFQSLGAEEVSTSVFEPKLYSLEHVNWADWGNIMVIAPCTANVIGKMAGGIADEVLSTQAMAFSGPILLAPAMNVKMYNNKAVQKNIAYLKSIGVNFIGPEVGHLATLITATGRMSDPAVIFSKTRQILLGRNDLKGKRVVVGAGPTREPLDPVRYISNYSSGKMGYALAEAAIGFGADVTLISGPSNIPVPPGVNIIRVETASQMQSEIERFCIGADYLYMAAAVSDFKPASFQKQKIKRTEQGLKLVLHPNPDILKSLGKNRPQIVVGFALEIENLEARAFDKLRDKRLDMIVANNPSEEGVGFGSDYNRVTILTRQGKPVKLEKLPKFDVAIRIIEESIKLRKRDVPKNKR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should contain one or more Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains that are essential for its function.2. We are looking for a protein with broad substrate specificity for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.3. The protein design must be able to perform FMN binding, metal ion binding, phosphopantothenate--cysteine ligase activity in order to contribute to coenzyme A biosynthetic process, pantothenate catabolic process.4. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..5. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.6. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.7. We require a protein that can specifically bind to the metabolites produced in the Cofactor biosynthesis, coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway. The designed protein sequence is ",
    "response#1": "MNIVVGVAGGIAAYKTVHLVRLLTKGGHDVTVVPTEDALRFVGLPTWEAISRHAVTTSVHDDVAKVRHVALGQNAELVIVAPATANSIAKMAAGLADDLLGTTLLATEAPVLIAPAMHAEMWRNRATQANITTLRERGVHVVGPADGELAGGDSGPGRMSEPEEIFAALAARFSAPGALAGVRVVVSAGGTREPIDPVRFLGNRSSGRQGAALAAEAAARGAEVTLVSANISGEVLAEVRHPSIRVVPVGTAAELAEAMTHESALADVVVMAAAVADYRPAAVSERKLTKEGGGIPAIELVENEDIVAALASVRRSGQLVVGFAAETPEDASELLARARRKQQRKGVDLLIVNEVGWERGFESPENAVHIRLLTPGGELAGRHVVISAGGTREQLDPVRFLGNASSGRQGVALARTAALRGARVTLVAAHITEPVPSGADVVRVGSTAELAEAMLALQPTADALVMAVAAADFTPERVADGKIKKDGSGTLELRLTETTDVLAALSASGAGPAGQVIVGFAAETADDEAHLLELGRAKLARKGCDLLVLNNVSGGAVFGSPDNSVTILEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLLRGADTPALAGRHVVISAGGTREQLDPVRFLGNASSGRQGVALARTAALRGARVTLVAAHITEPVPSGADVVRVGSTAELAEAMLALQPTADALVMAVAAADFTPERVADGKIKKDGSGTLELRLTETTDVLAALSASGAGPAGQVIVGFAAETADDEAHLLELGRAKLARKGCDLLVLNNVSGGAVFGSPDNSVTI",
    "response#2": "MNIVVGVAGGIAAYKTVHLVRLLTKGGHDVTVVPTEDALRFVGLPTWEAISRHAVTTSVHDDVAKVRHVALGQNAELVIVAPATANSIAKMAAGLADDLLGTTLLATEAPVLIAPAMHAEMWRNRATQANITTLRERGVHVVGPADGELAGGDSGPGRMSEPEEIFACLHALHTQDHFGLAGRHVVISAGGTREQLDPVRFLGNASSGRQGVALARTAALRGARVTLVAAHITEPVPSGADVVRVGSTAELAEAMLALQPTADALVMAVAAADFTPERVADGKIKKDGSGTLELRLTETTDVLAALSASGAGPAGQVIVGFAAETADDEAHLLELGRAKLARKGCDLLVLNNVSGGAVFGSPDNSVTILDPNGTKTLEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLLEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLS",
    "response#3": "MNIVVGVAGGIAAYKTVHLVRLLTKGGHDVTVVPTEDALRFVGLPTWEAISRHAVTTSVHDDVAKVRHVALGQNAELVIVAPATANSIAKMAAGLADDLLGTTLLATEAPVLIAPAMHAEMWRNRATQANITTLRERGVHVVGPADGELAGGDSGPGRMSEPEEIFAAAVAALAPRRGLAGRHVVISAGGTREQLDPVRFLGNASSGRQGVALARTAALRGARVTLVAAHITEPVPSGADVVRVGSTAELAEAMLALQPTADALVMAVAAADFTPERVADGKIKKDGSGTLELRLTETTDVLAALSASGAGPAGQVIVGFAAETADDEAHLLELGRAKLARKGCDLLVLNNVSGGAVFGSPDNSVTILTSDDVEILEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLGPELRLERGSAQTLARRVLAAL"
  },
  {
    "reference": "MKAKETRKNSPRWWGWGTMLLGMLLICSATDQLWVTVYYGVPVWKEATTTLFCASDAKAYDAEVHNVWATHACVPTDPNPQEVVLENVTENFNMWKNTMVEQMHEDIISLWDQGLKPCVKLTPLCVTLNCTTKPTTDDNATVEEKMEGGVKNCSFNVTESIGNKVRKEHAIFNNLDVVPINDRSSNSSNSSYMLINCNTTVITQACPKVSFEPIPIHYCAPAGFAILKCNDKKFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSENLTNNVKTIIVQLKDPVEINCTRPNNNTRRSIHIGLGRRFYTRQIIGNIKQAHCNVSGEKWNDTLHQIVKKLKEQFKNTTIVFNQSAGGDPEIVMHSFNCGGEFFYCDTTRLFNTTWNDTNNSGLNNETITLPCRIRQIINRWQEVGKAMYAPPISGQINCSSKITGLLLTRDGGTNDTNNTNKTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGVIGAMFLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARILAVERYLKDQQLLGIWGCSGKLICTTTVPWNTSWSNKSIERIWNNMTWMEWEREIDNYTGLIYTLLEESQNQQEKNEQELLELDKWASLWNWFDITKWLWYIKIFIMIVGGLIGLRIAFTVLSIVNRVRQGYSPLSLQTHLPAPRGPDRPEGIEEEGGERDRDRSGRLVTGFLALIWDDLRSLCLFSYHRL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must have at least one Retroviral envelope protein GP41-like domain.2. The protein must exhibit the following characteristics: Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Transmembrane protein gp41: Acts as a class I viral fusion protein; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes.3. The protein should be optimized for stability and expression in virion membrane, viral envelope, host cell plasma membrane where it will participate in viral protein processing, virion attachment to host cell.4. The designed protein should contain at least one Di-leucine internalization motif motif to achieve its intended function.5. The protein design should emphasize Basic and acidic residues compositional bias.6. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "MRVKGIRKNYQHLWRWGTMLLGMLMICSATEQLWVTVYYGVPVWKEATTTLFCASDAKGYDTEVHNVWATHACVPTDPNPQEMVLANVTENFNMWKNDMVDQMHEDIISLWDQFLKPCVKLTPLCVTLNCSTLSTKCNNTGTNNSLWVTVYYGVPVWRDADTTLFCASDAKAHETEVHNVWATHACVPTDPNPQEIQLENVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTNANLTHNIINGNSTIGNITDNITDEVKNCTFNMTTEIRGKQQKVYALFYTLDIVHMGGNDTEYRLINCNTSVIKQACPKVTFDPIPIHYCAPAGYAILKCNEKKFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKNIIVHLNKSVIINCTRPSNNTRMSTRIGPGQMFYRTGDIIGDIRKAYCEINGTEWNETLNQVTKKLKEHFNKTIIFQPPVGGDLETTMHHFNCRGEFFYCNTTKLFNSTNMEGFNTTIILPCRIKQIVKMWQGVGQAMYAPPISGIVNCISNITGILLTRDGGVNETFRPGGGNIKDNWRSELYKYKVVQIEPLGIAPTRAKRRVVNREKRAVGIGAVFLGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLKLTVWGIKQLQARVLAVEKYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNRTLDDIWGNMTWLQWDKEISNYTNTIYDLIEKAQNQQEQNEQDLLALDKWANLWNWFDISKWLWYIKIFIMIIGGLIGLRIVFAVLSIINRVRQGYSPLSFQ",
    "response#2": "MRVKGIMKNCQHLWRWGIMLLGILMICSATENLWVTVYYGVPVWKEASTTLFCASDAKAYKTEKHNVWATHACVPTDPNPQEVVLENVTEEFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDYAEETNNSTPATPTPTTSGTKGSTNGSGGAIEEREIKNCTFNITTGMRDKVQKEYALLYKFDIEPIADSSTKSGTSNTRYRLIRCNTSVITQACPKISFEPIPIHYCAPAGFAILKCNDKKFNGTGTCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIMIRPKNISENDDIIIVQLNKTVLINCTRLNNNTRKSIPMGPGKAWFTTGRVIGDIRQAHCNISRKDWNNTLRQVATKLRELFNKTIIAFNQSTGGDPEIAMHSFNCGGEFFYCNTTQLFNSTWYGNETEYKGNETKDNETTIITLPCRIRQIVNRWQEVGKAMYNPPIEGLIRCSSNITGLLLTRDGGNITNETITTETFRPQGGNMKDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGFGALFLGFLGAAGSTMGAASLTLTVQARLLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNNSWSNRNLSKIWDNMTWMQWEKEIDNYTDIIYQLLEKSQNQQEKNEQELLELDKWGSLWNWFDITNWLWYIKIFIMIVGGLIGLRIVFAVFSIVNKVRQGYSPLSFQT",
    "response#3": "MRVMGTQRNYPPFWRWGMIIFWIMMICNAQKLWVTVYYGVPVWRDANTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEIDLVNVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTRANFNATGNNYTSEQMQNCSFNTTTVVQDKISKQHALFYRLDIVPLNDSSENNGSREYTLINCNTSTITQACPKVSFDPIPIHYCAPAGYAILKCNNKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEKEIIIRSENLTNNARTIIVQLNESIGIVCTRPNNNTRKSIRIGPGRTVYATDRIIGDIRQAHCNISEEQWNKTLQKVSEKLKEHFPNSTIKFNQSSGGDLEITTHTFNCRGEFFYCNTSKLFNGIYNSTGNNSTGNSSSQNKTITIPCRIKQIINMWQKVGRAMYAPPIAGNITCNSSITGLLLVRDGGQDNNTNGTETFRPGGGNMKDNWRSELYKYKVVEIKPLGIAPTGAKRRVVGREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQFLGLWGCSSKVICTTAVPWNSSWSNKTYEEIWDNMTWMQWDKEISNYTSIIYRLLEVSQNQQDKNEKDLLELDKWASLWNWFNITKWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSLQTLIPNS"
  },
  {
    "reference": "MAAAVLNPGFFLLILLLSFSISPSLQYVSESEPLVRFKNSVKITKGDLNSWREGTDPCSGKWFGIYCQKGLTVSGIHVTRLGLSGTITVDDLKDLPNLKTIRLDNNLLSGPLPHFFKLRGLKSLMLSNNSFSGEIRDDFFKDMSKLKRLFLDHNKFEGSIPSSITQLPQLEELHMQSNNLTGEIPPEFGSMKNLKVLDLSTNSLDGIVPQSIADKKNLAVNLTENEYLCGPVVDVGCENIELNDPQEGQPPSKPSSSVPETSNKAAINAIMVSISLLLLFFIIVGVIKRRNKKKNPDFRMLANNRENDVVEVRISESSSTTAKRSTDSSRKRGGHSDDGSTKKGVSNIGKGGNGGGGGALGGGMGDIIMVNTDKGSFGLPDLMKAAAEVLGNGSLGSAYKAVMTTGLSVVVKRIRDMNQLAREPFDVEMRRFGKLRHPNILTPLAYHYRREEKLVVSEYMPKSSLLYVLHGDRGIYHSELTWATRLKIIQGVAHGMKFLHEEFASYDLPHGNLKSSNVLLSETYEPLISDYAFLPLLQPSNASQALFAFKTPEFAQTQQVSHKSDVYCLGIIILEILTGKFPSQYLNNGKGGTDIVQWVQSSVAEQKEEELIDPEIVNNTESMRQMVELLRVGAACIASNPDERLDMREAVRRIEQVKT",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Incorporate a signal peptide in the protein design.2. The designed protein should have protein serine/threonine kinase activity within plasma membrane, pollen tube tip, cytoplasm to regulate pollen tube guidance, protein phosphorylation.3. The designed protein have Protein kinase domains with specific sequence motifs that are crucial for its activity.4. The Cytoplasmic, Extracellular topological domain of the protein should be designed to be flexible or rigid.5. The protein must exhibit the following characteristics: Key receptor for sensing species-specific attractants in cooperation with other pollen receptor-like kinases.6. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.7. The ATP binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "DPLKLGIIDNKTLRLITESSGSPSKAEKVDNTLREKLVYFSDHFDPKLFLSRIHQDTTAADLEAGALGLKSDLKGRNLQRKQLVKDNFDCFVSCKTTIDDIESKLKRIEEDPEGSGTTHLFNCMKSVTSRANLAFEPLFERQAQAEKIRSVQGMLQRFRTLFNLPSIIRSSISKGEYDLAVREYKKAKSIALPSHVNILKRVLEEVEKVMLEFKGTLYKSMEDPKIDFTSLENTVRLLLELEPESDPVWHYLNVQNHRIHGLLEKCTYDHEARVEILRNDTHEKAISDAKWQQIQQNGVSYSDTASSNENNAVQVDLQSVEFPSEEIDILKGRYIKRLTAVLVHHIPVFWKTAISIFSGKFAKSSQVTDTSANKAEEKVTEARYSTHSLEEVAGMIRKTISVYEAKVNSTFCDFDESCILRPFMSDAINEVSKACQAFEAKESTPHSAVVALRKIQAEITKIYIQRLCSWMRASTEGISKEETWIPVSILERNRSPYAISYLPLAFRSVIVSGMEQVNLMILSVKSEAAKSEDMFAQIEEIIISVRLAFLNCFLDFAAHLEQIGADLSQSTSRQDNY",
    "response#2": "TNFAFRGFNGNQSKIRIEGAAMIKPDGLLRLTDRKSNVTGTAFYHKPVRLLNRNSTNVTIRSFSTSFVFVIIPSSSSNKGFGFTFTLSPTPYRLNAGSAQYLGVFNKENNGDPRNHVFAVEFDTVQGSRDDNTDRIGNDIGLNYNSRTSDLQEPVVYYNNDDHNKKEDFQLESGNPIQALLEYDGATQMLNVTVYPARLGFKPTKPLISQHVPKLLEIVQEEMYVGFTASTGKGQSSAHYVMGWSFSSGGERYRSGPWAKTRFTGIPLMDDTFTGPVSVQQDTNGSGSLTYLNRNDRLQRTMLTSKGTQELSWHNGTDWVLNFVAPEHSCDYYGVCGPFGLCVKSVPPKCTCFKGFVPG",
    "response#3": "FKKTGIIGTGGFGTVFKGKLPNSDPIAVKKIIPSSRQGVREFVAEIESLGKLRHKNLVNLQGWCKHKNDLLLIYDYIPNGSLDSLLYTVPRRSGAVLSWNARFQIAKGIASGLLYLHEEWEKIVIHRDVKPSNVLIDSKMNPRLGDFGLARLYERGTLSETTALVGTIGYMAPELSRNGNPSSASDVFAFGVLLLEIVCGRKPTDSGTFFLVDWVMELHANGEILSAIDPRLGSGYDGGEARLALAVGLLCCHQKPASRPSMRIVLRYF"
  },
  {
    "reference": "MPTPIRTILLDIEGTVGSISFVKNVLFPLAKQQYAEYVHKHMDDASVRVFGNSLDEIVDKLNTLHDSGSKDQAFKALQGSIWKDAYENGKVVAHLFPDVVPLLKRAADKGVRVCIYSSGSVPAQKLYFHYSEYGDLSNYISEYYDTSIGPKVEADSYKRIVGSDDPATWLFLSDNVHELDAARQSGLKVGLAVRPGNEPVSSSGYSEYGSFDSLL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a low Km value for 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-   dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate.2. The Mg(2+), substrate binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein should have acireductone synthase activity, 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity, magnesium ion binding within nucleus to regulate L-methionine salvage from S-adenosylmethionine.4. The protein design should consider the endogenous proteins and enzymes present in the Amino-acid biosynthesis, L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6 pathway to avoid interference with native metabolic processes.5. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "LSGTDLAKEIKIRLQEEVKHLMETNKGFAPGLAIVQVGDREDSNVYIRMKLKAASEIGIDAKHIRFPSSITEDELLNGINTLNEDINVHGIIVQMPLDSSNKIDNNKATDAVSPLKDVDGFAKGGLHTINAGKVARGVLDDSFIPCTPHGCLELIKKSGITIAGSRAVVVGRSRIVGMPMAELLIWNNATVTTCHTKTKEMDKVISEADILVVAAGRPQMIKGDCIKPGAVVIDCGINSIPDSSRKSGQRLVGDVDFDSAKLKASHITPVPGGVGPLTVAMLMHNTVQAAGPLKDVDGLHTINAGKVARGVLDDSFIPCTPHGCLELIKKSGITIAGSRAVVVGRSRIVGMPMAELLIWNNATVTTCHTKTKEMDKVISEADILVVAAGRPQMIKGDCIKPGAVVIDCGINSIPDSSRKSGQRLVGDVDFDSAKLKASHITPVPGGVGPLTVAMLMHNTVQAEGVELTVFKPTFAVPSAGIHPGMVPLLMKDFGIDHIINAGGGIHGHPNGAAGGGRAFRAVIDAVLEAEPVEEKAKRSPDLKLALEKL",
    "response#2": "MTGELLATYILADPGCDAEKRAEQIAIGLTVGSWTDLPLLKQEQLKKHKGRVVNVEETESELGEKQATVTIAYPEANFTNDIPAVLTTVFGKLIGPTGEEVPVFCGLSGTDLAKEIKIRLQEEVKHLMETNKGFAPGLAIVQVGDREDSNVYIRMKLKAASEIGIDAKHIRFPSSITEDELLNGINTLNEDINVHGIIVQMPLDSSNKIDNNKATDAVSPLKDVEGMDFVFKPTFAVPSAGIHPGMVPLLMKDFGIDHIINAGGGIHGHPNGAAGGGRAFRAVIDAVLEAEPVEEKAKRSPDLKLALEKYV",
    "response#3": "MRELLATYILADPGCDAEKRAEQIAIGLTVGSWTDLPLLKQEQLKKHKGRVVNVEETESELGEKQATVTIAYPEANFTNDIPAVLTTVFGKLIKKDAIEVPAIAKEENVGIEGIRNLLQVQDRPLLMSIFKGMIGRNIGYLKTQLRDQAIGGVDIVKDDEILFENSLTPLIKRIESGKEVLQSVYETYGHKTLYAVNLTGRTYDLKENAKRAVVAGADILLFNVFSYGLDVLQALVEDDEIPIPIMAHPAVSGAYSSSKLYGFSSPLLLGKLLRYAGADFSLFPSPYGNVALEKEEALLITAALTEEDHYFKKSFSVPSAGIHPGFVPFILRDFGKDVVINAGGGIHGHPNGAQGGGKAFRAAIEATLQGKPLHEVDEINLHSALPLKDVDGLHTINAGKVARGVLDDSFIPCTPHGCLELIKKSGITIAGSRAVVVGRSRIVGMPMAELLIWNNATVTTCHTKTKEMDKVISEADILVVAAGRPQMIKGDCIKPGAVVIDCGINSIPDSSRKSGQRLVGDVDFDSAKLKASHITPVPGGVGPLTVAMLMHNTVQAKKERGL"
  },
  {
    "reference": "MVQAWYMDESQEDQRAPHRQQPNRAVSLEQLRRLGVVYRRLDADNYETDPCLKEIRRAENYSWMDIVTIHKDKLPNYEEKIKTFYEEHLHLDDEIRYILDGSGYFDVRDKDDKWIRISMEKGDMITLPAGIYHRFTLDENVCCCRLP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should incorporate a binding pocket that specifically recognizes Ni(2+), Fe(2+) to enhance enzymatic efficiency.2. The Ni(2+), Fe(2+) binding site should be stable and able to withstand changes in environmental conditions.3. The designed protein should interact with enzymes and substrates within the Amino-acid biosynthesis pathway to enhance or regulate its activity.4. The protein can be expressed in plasma membrane and perform iron ion binding, acireductone dioxygenase (Ni2+-requiring) activity, nickel cation binding, acireductone dioxygenase [iron(II)-requiring] activity without triggering undesirable cellular responses.5. For general function, the protein need meet that Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site; Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.6. We are looking for a protein with broad substrate specificity for 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-   methylsulfanyl-2-oxobutanoate + formate + 2 H(+). The designed protein sequence is ",
    "response#1": "MLNINSANDVAVGQQIDLATYLAEQGIDFEQLLPQPTHDDELTLLLPKERQAEILKTLPESFQHDLQAGAEHSGYQSWDVISLSKTVPDMQKRFVKLYSKTYHSEDEVRFFVHGSGLFSLHIGDDVYAVLCEKGDLISVPEHKAHFFTLTENRQICAIRVFDTKSGWVAHPYQN",
    "response#2": "MRGDRPRQRPRGDGRVPAGPVGAPGRACGRGAPAPAGATGPPHPCRAPGAACPRRPRRAPCPRAHGRTSGAGRPPCPRRAGRPPPPAGTGATRVPTGGPGGPAAAGGPRRPGRHRPARAPRPGRPGRRGHAGARVRRGRCRAPDRVPRPGRPGRPGQQRPGGRPGRRGQAGARVRPGRRGRRAGPRRAGGPGRARGRAGRGGGAGAGAGAGPGGPGGPGRAGRAGRAGQAGQAGQAGQAGRAGQAGRAGRAGRAGRAGQAGQAGQAGRAGQAAVAGRAGRAGRAGQAGRAGRAGRARRARRAGRGRGAGRAGGAGAGRRRRAGGAGGGAGGARRRRGAGGRRGRGPGGRGRAGRAGRGGRGGRGGGAGGRGGGGGGAGGRGGAGGRGGRGGGGGRGRGGGGGGGGGGGGRGGGGFGGRGGGGGGGGGGGGRGGGGGRGGGGGGGRGGGGGRGGGGGGRGGGGGGRGGVGG",
    "response#3": "MTLHFNGNSSNGSSKSKRSFGNGGSSANGINNGSNNGSNGSSKKKKRRYWNPTMILQLLKQDPRQRITKIELPTNMVIKDAQLIVSQLKDLVHLNLTGNKIRKLEPVFNCLQNLQVLNLSHNQNFQDDLIGLCEIETLEQLHVSGNAIDTKRVFGYLPRIRVLNLNENTFCLQDENYLLALYRIDRLIYLDGIEISDSEREQAIMGGAHSDDENDESEDDEQVETSSSGDEDQQNETHNENDHINERNGSRFHPQMYIHSNQDEDEEEEPSEIIVNGSPVPQRRRRPNVFGGPPSPDPTREGPSLTGAPGVQKRLTRRKSFRIFPKHENRDNTDNEENGNGDNSTHNGTSGTSQVPTDRTLRRRSFSSHEPVDQTNGSPDRDSTQNNGNDQASTTSSSRRNRSFSNVSPNQNHRPAPTVSSSPMSSSMTTTTTSSSTPTTSTSSNLTSGSSSNTSSPTTTTTITTTSSSSSSSTLSSSSSSSSAITSSSSGSSSSLSSSSSSSSSSSTTTTNSSTPPVSSSSASSSSASSSSSSSSSSSSSSSSSSSSSSTSTSTSTSTPTPTPTTTTTTTTTTTTTANTTTTTTTTTTATTAATTTATTTSTTTTSTTSSSSSSSTSSSSTSTTPPSISSSSSSSSSSSSSSSSSGSSSSTSSSSSS"
  },
  {
    "reference": "MKKSALQKARAAYKPKLPVVLTSAVKVVEGRPTESVADQAAIKSLFPNTYGLPEIRFEKNPAPAPGKPVNVGVILSGGQAPGGHNVICGLFDGIKKIHRDSRLFGFLMGPGGLVDHKYIELTAPIIDEYRNTGGFDIIGSGRTKLEREEQFEKGLIIIKELGINALVIIGGDDSNTNAAVLAEYYKAHNAGVQVIGCPKTIDGDLKNDVIETSFGFDTATKVYSEVIGNIQRDCNSAKKYWHFIKLMGRSASHIALECALQTQPNICLISEEIEQRDMSLDQIVSYIGDIVIARAADGNNFGTVLIPEGLIEFIPEYKRLIRELNELLGANKAVVKNMNSEDLTAFILGNLSAENVAALKKLPEEVSRQLMLDRDPHGNVQVSLIETEKLLSGLVADYVAKSEAFAAAKGKFSALHHFFGYEGRCADPSNFDADYCYSLGFNAAALINAGVTGYMSSIRNLVKPAVQWIPGGIPITMMMNVERRNAEDKPVIQKALVRLDGKPFLKFAEQRDSWAHNTCYVYPGPIQYFGPSEVCDQPSMTLKYEHQGK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The Mg(2+), substrate, diphosphate binding site should be located in a region of the protein that is accessible to the ligand.2. The protein should be optimized for activity in D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 3/4, glycolysis, Carbohydrate degradation pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The protein can be targeted to cytoplasm and perform diphosphate-fructose-6-phosphate 1-phosphotransferase activity, ATP binding, metal ion binding.4. The protein must bind to Mg(2+) in order to perform its enzymatic function.5. The protein must exhibit the following characteristics: Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis; Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis.6. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for beta-D-fructose 6-phosphate + diphosphate = beta-D-fructose   1,6-bisphosphate + H(+) + phosphate. The designed protein sequence is ",
    "response#1": "IGILTGDGDCPGINAVIRAVVRKSLNLGNEVIGFRKSWKGILEKDIYPLDINSVSDIIGKGGTILGSSRTNPFKYEDGPEKVVRHLRDLNVNVLVAVGSIDTLMVAEKLYERGCPVIGIPKTIDNDLPLTDRTFGFDTAISTVTEAIDRVHSTAESHDRVIIMEVMGRYTGWIATYAGIAGGADCILVPEKPFSIEDVCALIRRRHQRGKDFSIIVVAEGARPKEIDHFVVKTNRVDEFGHKQLGGIGSYIAEEIERLTGFETRVTILGPVQRGGAPTAYDRTLATLFGVKAAEVAILVGGGPAPGINGVIRSITIEAVNSGLRVIGIYDGFKWLARGDSSRTTELHTENVSRIHFLGGSILRTSRDNPTETPAKMEATVRALTQLEVDYLITIGGDDTAYSSQRLHELVGVQIQIAHVPKTIDNDLPLPGDMPTFGYETARHVGAEIVRTLMEDAQTTNRWYYVVSMGRKTGHLALGIGKAAGATLTIIGEEFRNRKISFTTVCDMLETSAIKRMAMGRPYGVAVLAEGILDRIDKNELMRVADLDYDAHGNIRFSEIDLGRMLKNEVSRRMKERGIPATLVAKDIGYELRCASPIPFDREYVQDLGYAAVRLLVGQSGGPTAVINGSLYGVVSEGLKKTDEIGHVYGMVNGIEGFLNDNYMDMKSLEETNELELLRTTPGAFLGSCRYKLPEELDNEVYPTLFQKFEALDIGYFFYIGGNDSMDTVSKLSRYAEKVDSSIRFIGVPKTIDNDLVETDHTPGFGSAAKYVATTVREIATDASVYDNKKSVTIVEIMGRHAGWLTAASVLARKFEGDNPVLIYLPEVAFDQDAFVAKVEEALKTTPNLVVCISEGINDGKGTFICEFASEVGTDNFGHKMLTGSGKYLENLVKAKLGVKV",
    "response#2": "IGILTGDGDCPGINAVIRAVVRKSLNLGNEVIGFRKSWKGILEKDIYPLDINSVSDIIGKGGTILGSSRTNPFKYEDGPEKVVRHLRDLNVNVLVAVGSIDTLMVAEKLYERGCPVIGIPKTIDNDLPLTDRTFGFDTAISTVTEAIDRVHSTAESHDRVIIMEVMGRYTGWIATYAGIAGGADCILVPEKPFSIEDVCALIRRRHQRGKDFSIIVVAEGARPKEIDHFVVKTNRVDEFGHKQLGGIGSYIAEEIERLTGFETRVTILGPVQRGGAPTAYDRTLATLFGVKAAEYAQAGGVTAVINASAAAVIEAAREHPQAIGTVYGARNGILGVLSETLVDTTPLTPAELAQLALTPGGAFGSCRFDLDPAEANPAQYDRLFEVFASYRIGAFLYNGGNGSMDIVARVAAAARARAYPLCCIGVPKTVDNDLEGTDCSPGFGSAAKYLATAMRESGLDLAAMSSRRGRVFVMEVMGRNAGWLAAATALAARNEDEPPHIVLLPEVAFDEEAFLARVKAVVERLGYCAVTVAEGIRRADGTLVMEQSHDALGHVQLGGAGQCVARLVSARFDYKHSWGAIKELIGILTGDGDCPGINAVIRAVVRKSLNLGNEVIGFRKSWKGILEKDIYPLDINSVSDIIGKGGTILGSSRTNPFKYEDGPEKVVRHLRDLNVNVLVAVGSIDTLMVAEKLYERGCPVIGIPKTIDNDLPLTDRTFGFDTAISTVTEAIDRVHSTAESHDRVIIMEVMGRYTGWIATYAGIAGGADCILVPEKPFSIEDVCALIRRRHQRGKDFSIIVVAEGARPKEIDHFVVKTNRVDEFGHKQLGGIGSYIAEEIERLTGFETRVTILGPVQRGGAPTAYDRTLATLFGVKAAE",
    "response#3": "IGILTGDGDCPGINAVIRAVVRKSLNLGNEVIGFRKSWKGILEKDIYPLDINSVSDIIGKGGTILGSSRTNPFKYEDGPEKVVRHLRDLNVNVLVAVGSIDTLMVAEKLYERGCPVIGIPKTIDNDLPLTDRTFGFDTAISTVTEAIDRVHSTAESHDRVIIMEVMGRYTGWIATYAGIAGGADCILVPEKPFSIEDVCALIRRRHQRGKDFSIIVVAEGARPKEIDHFVVKTNRVDEFGHKQLGGIGSYIAEEIERLTGFETRVTILGPVQRGGAPTAYDRTLATLFGVKAAERVGLLTGGGDCPGLNAVIHAVVKKGINHYGDEFVGFLEGWRGVIENNTTPLTLEKVDGILTLGGTILRTSRTNVRKIEGGIEKCIATLKANKIDALIAIGGDDTQSVTNALVQAGFPGVGVPKTIDNDLNGTDVTFGFDTAVSIATEAVDRLHTTAEAHNRVIVCEVMGRDAGWIALTAGVAGNAHVVLVPEKPIDIDHVCALLKYNHDHGKKYGIVVVAEGAKIPGAGQATLGGKVDSFGHARLSGIGQALAELIEEKTCYETRSVNLGHTQRGGTPTAYDRMLSTRYGLAAIDAALDGDFGKMVVLRGTDIVEVPLEEATTELKTVPPHRYDLAHSFFP"
  },
  {
    "reference": "MLINVRKFVNSIYRMAPKVVFVLGGPGAGKGTQCQNIVKEYGFVHLSAGDLLREERSKPESEYGELIEAYIKEGKIVPVDITCSLLERAMTNSEKDMFLIDGFPRNKDNLDGWNRRCANKVQLLFVLHFDCPLEICVERCLNRGAAGSARIDDNIESLKKRVNTFINETKPVIDYYKTQNLVKEIDGSRPKEEVFKDIRKAFDDIKAMGDI",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Has preference for UMP and CMP as phosphate acceptors.; Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP2. The designed protein should be involved in phosphorylation, 'de novo' pyrimidine nucleobase biosynthetic process, pyrimidine nucleotide biosynthetic process by localizing to nucleus, cytoplasm and exhibiting uridylate kinase activity, cytidylate kinase activity.3. The designed protein should be able to compete with endogenous proteins or inhibitors for CMP, a ribonucleoside 5'-phosphate, ATP binding.4. The protein should have a low Km value for ATP + dCMP = ADP + dCDP.5. The protein requires Mg(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "MKVEGKRHLVDSTQRLLDAVFQGDWATYKDLCDECLSAIEPQSMGHVILSHGEVFSD",
    "response#2": "MVILGIDPGSRITGYGVIRQQGRQLTYLGSGCIRTSSDELPMRLKLVYAGVSEIITQFNVDSFAMEQVFMAKNADSALKLGQARGAAIVAAVNRDLPVFEYAARQIKQTVV",
    "response#3": "MSGGGTPVAGRFLVDGFPRNEDGAXGXTXADQLLXFLXSXAAXAXVXLLXADXGYXQRXFXXSXGGXKXTXIXXXLDXEXXXAIXAXPXFLIDGFPRNQNXLQRXXGXVXDXDXLGXXIXGXGXNXVXYXGXXGXGXGXNXYXNXGXTXYXNX"
  },
  {
    "reference": "MNEPSAIIGTQTRPAPSPGDGRTNLLGLSREEISTALAALDVERYRADQIYGWIYAKGVTDFEAMTNLSKDLRSRLQDAFRIDRPGITADQQSVDGTRKWLLGFEDGNAAETVHIPEADRGTLCVSSQVGCTLTCAFCHTGTQRLVRNLEPGEIIGQIMLARDHLEEWPSAKEEKRLTNIVMMGMGEPLYNYDNVKKALEIAMDGDGVAISKRRITLSTSGVVPMIEKCGAELGVMLAISLHAVTDDVRDMLVPINKKWPIKELLAACRAYPAAKNARRITFEYVMLDGVNDSEADARELCRLIKGIPAKVNMIPFNPWPGSAFECSSPEAIKKFARVVEKAGYVATVRTPRGRDILAACGQLKTESERLRKSERPN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that contributes to rRNA base methylation by functioning in cytoplasm with 4 iron, 4 sulfur cluster binding, rRNA binding, metal ion binding, tRNA (adenine-C2-)-methyltransferase activity.2. The [4Fe-4S] cluster, S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand.3. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.4. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.5. The protein contains novel Radical SAM core domains that confer a unique function or activity.6. The protein's S-methylcysteine intermediate, Proton acceptor must be carefully engineered to ensure that the amino acids involved in adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "MPRRHLLDLSLAELRDWVVERGEKPFRAAQVWRWIYQGRAADFESMTDLSRSLRQHLAEDFVLFAGSIARHQTSPDGTEKLLIGLADGGEVECVLLRDGPRRSICVSSQVGCAMGCVFCASGLDGVDRNLTRGEILEQMLRLQHRLDHEERLSHIVMMGMGEPLANLERVLSALDVARSPEGLGISPRRITISTVGLPPAIDRLARHGVPYNLAVSLHAPEDELRNRLVPVNRKIGVEALMAAADRYFEASGRRLTFEYVLLGGLNDSPTQAAQLGHLLRGRGAMLNVIPYNPVAGLPYRTPTSTATSRFREVLERGGINVNFRVRKGGEIDAACGQLRRNRQATL",
    "response#2": "MEGLGEPSYRGGQILEWLWQKRATSIEGMSNLPVRLREHLEESFTLRSLSPAKSQGSRDSTRKFLFRLNDGRYIECVLIPASPALYGERSDRRTLCVSSQVGCAFGCKFCASGLDGFTRNLTADEIAGQVLLAEELSGEKVNNIVFMGMGEPLANLPQLLKALELITSPWGLGIGARHLTVSTSGLVPQIRKLADHPQQIRLAISLHGASNDVRDRIMPVNRKWPVEDLFKALSYWTKRKNQKITLEYILIDGINADPAQARLLAQHAESLRAKVNLIPYNTVDGLKWKRPPEEECHAFRDILAARGIPATLRLEKGHDIDAACGQLRLKEERAEKRQAREAAEAAIAAEIEAAEERLLAFGVKNYHAKSFTHFVYQLGVLEPSKMLSLSRDFRKRLQDCVDTRLLPIKKRLVSKDGTVKFLFEVRGPKSLEEIEAVYIPEKSRVTLCVSSQVGCKMGCRFCLTAQLGFRANLSAGDIVRQVMTVNADPELRPVTNIVFMGMGEPFDNLDEVRKATKILTHERGLKLSARKICVSTVGLADRIDSLTKEEPFRLAVSLNSSDDVSRNEVMPINRRWNLERLLESCLRYSQRTNKKVTFEYILMKGVTDRDEDLRNLVRLLSPIPCKLNLIPYNESPFTPFKRPTEERILEFHAGLLKAQFPVFIRKNRGNDIFAACGMLKRQSE",
    "response#3": "QAGRGTLCVSSQAGCALDCSFCSTGKQGFNSDLTSAEIIGQVWIANKSFGTVPGKIDRAITNVVMMGMGEPLLNFDNVVTAMSIMMEDFGYGISKRKVTLSTSGVVPMIDKLGETTDVSLALSLHAPNDELRNQLVPINKKYPLKMLLDACRRYITRLGKERVLTIEYTLLSNVNDQPEHAEQMIALLKDFPCKINLIPFNPFPHSGYERPSNNSIRRFQDMLHKGGFNVTVRTTRGDDIEVAGPDGRKMSKSYNNYISPDEMVAKVKGVLESSPEALTEWLRAAGQPAFRAKQIRQWLIQRRISSFDQMSDLPAALRTLLQNERSSQIGCKMGCVFCASTKERFGRNLEKHEMLDQIYAVNEYLNTRIDNVVLMGIGEPLENYDNVTGFIEMISDEKGYGMSIRSITLSTCGIVPKIYQLGEDHFPINLAISLHNPFNEERKKLMPIGKKYSIEEILDACDYFFKETGRRVSFEYTLIQDENDEAVARARAESARAVAAAQ"
  },
  {
    "reference": "MKVFSKEQIYEGDKITEERQKLPPSELMERAGLQIFNWMHMRMQGAQVPIHIFCGIGNNGGDGLVLARHLITHGYNVETYVVNCGNSRSKDFLTSYDKVKEVTKKWPQLLKCESDFPEIYKDDIIVDAIFGIGLNRSIEDWIKALFHHFKASEAFTLSVDIPSGLYTDKPVEDVNGLVKANYTLSFQSPKLVFFLPETADYTMQWEVIDIGIDRDYLMTTETEFELISKNEVLPMYTPRTKFSHKGNFGHGLIIGGSYGKVGAVQLATRSLLASGAGMATAYVPECGYIPLQTSLPEAMVITDTNYKTITDISFDISPSAIGIGVGTGTDATTVKAYEAFLKNNKAPLVIDADAINMLSENKKLLKLVPENSILTPHLIELERLIGKWSNDFEKLEKASVFSSKYKVILVIKGAHTITVYGQKKFINVTGNPGMATAGSGDVLTGVITGLLCQNYLPIEATLFGIYLHGRAGDLAVEEFGYQSLLAGNIIDYLGEAYIDLFKQPEQPQEETETEEDA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.2. The protein should have a high affinity for K(+), AMP.3. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.4. The protein should have a fast turnover rate for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.5. The designed protein must be optimized for ADP-dependent NAD(P)H-hydrate dehydratase activity, NADHX epimerase activity. The designed protein sequence is ",
    "response#1": "MRQLDSETIQQLGIPAIALMENAGRVIAEEIITLCRERGGAGCRNAAERNDTGLREAALGGTGKAFKVSADHALTLASAAAERWFILVGKGNNGGDGLAAARYLRETGIAVTLVYAVPPESLSGEAALQRDAAAAMGLPAVVYGRDRLDLAECSGILDALLGTGGAGAPRGAYAELIAAANSSGKVIVSADIPSGLNADTGETHEPCIHASVTVCLAFLKRGLLQYPGAGAAGHVKVRAIGIPTALAGPGDLKYCLQKRPGESKKGDYGRIIIIGGSAEYSGAPALTALAALRTGADIVTVLVPERIEVPVRSFSPNLIVKSYPSDHLNRSALKENINLIEKHDVIAVGPGLGLNEDTILAVKEMLRLFDKPMVIDADGLKAIKGSLDLIYGKKAVLTPHAGEFKILFNAEPPRNLEERMKKVKELAGKYSTTILLKGHIDVISNGIKVKANPTGNPAMTVGGTGDVLTGIAATFLAHTEDPLYSAAGAAFLNGLIGDYVSSFKGAHITATDIIDYIPVILSRVSGPGDLKYCLQKRPGESKKGDYGRIIIIGGSAEYSGAPALTALAALRTGADIVTVLVPERIEVPVRSFSPNLIVKSYPSDHLNRSALKENINLIEKHDVIAVGPGLGLNEDTILAVKEMLRLFDKPMVIDADGLKAIKGSLDLIYGKKAVLTPHAGEFKILFNAEPPRNLEERMKKVKELAGKYSTTILLKGHIDVISNGIKVKANPTGNPAMTVGGTGDVLTGIAATFLAHTEDPLYSAAGAAFLNGLIGDYVSSFKGAHITATDIIDYIPVILSR",
    "response#2": "MRQLDSETIQQLGIPAIALMENAGRVIAEEIITLCRERGGAGCRNAAERNDTGLREAALGGTGKAFKVSADHALTLASAAAERWFILVGKGNNGGDGLAAARYLRETGIAVTLVYAVPPESLSGEAALQRDAAAAMGLPAVVYGRDRLDLAECSGILDALLGTGGAGAPRGAYAELIAAANSSGKVIVSADIPSGLNADTGETHEPCIHASVTVCLAFLKRGLLQYPGAGAAGHVKVRAIGIPTALADRLRPRARNTHKGSNGSAGILGGAASMVGAAFLAGRAALRLGSGRVYLGLLDGQAPAIDPLQPELMLRQADALLAAPLTALACGPGMGSGDEAHEWLRRACSLPLPLLLDADALNLLAAEPSLREEVLARAAPTLLSPHPTEAARLLDRDTATVQADRPRAALELAARYRAVTVLKGCGSIVAEPGGEWFVNTSGNPGLATAGTGDVLSGFIVALLAQGWPALEAMLAAVHLHGIAADERVAAGCGPVGLSAGELIPDSHKYTRGLVTIIAGDMPGASALAASAAMRSGAGVVRLQAREFVPGVPAAVIQMPGDPLDRLDDKRIGALLLGPGLRSGRELLDAALASGHPLVLDAGALAMLADGGPERIEGAILTPHDGEFQKLFGALEGSKVDRARASAAQSGAVIAYKGPDTVVAAPDGRAAIHGTAPPWLGTAGSGDVLAGIIAAERAAGKEPFEAACAGVWRHAEAARRAFDVVAVLAAEQAHQHDLESGVLMDRAATGLARAVVSLLQESRGQVRGAHIAIAVGGGNNGGDALFAGAKLAQRGVSVTAVCVATSAHNAGVAALLRANGRVVQWHEVESDLSQQNIFLDADVIIDGIVGIGGSGVLRPAAAAMVELINASGALVVAVDLPSGVAADTGEIAEIAVAADLTVTFGCLKTGLLVSPGSDFAGGLRVVDIGI",
    "response#3": "MRVLDLNCEYFGLSRLQLMENAGRSVAEEIKKRFEGGKVAIFAGTGNNGGDGFVCARHLEGFDVEVYLVSREIRSEIXXRNLEILERAGYKIREVGETDAEIIVDALLGTGLKGRPRADYARAIEIINSTKAFKVAVXVPXGLDSDTGSYEVAVKADLTVTFHKLKPGILKAKEICGEVVVKDIGIPRAFEHAHLGVDLARRRSPEGHKGTYGHVLLAAGTLRMSGAGLLAARAALRTGCGLVTWALPKKLLPSVIGVVPELMLADVGGSEEGNWVAETVSELLKLSEERDVIAVGPGLGRFPGDTDWLRSLWEGSDCPLVIDADALNILADSDYKSWVRRHSVILTPHPGEMARLANLTTAEVQRDRIGLAMDYAKEHGVTLVLKGAHTVIATPEGQAYVNTTGHPGMGTGGAGDVLTGMISSLLAQGLHATQAAAFGVYLHGLAGERAARQRNNPAAIIAGDIIEAL"
  },
  {
    "reference": "MASKPFYPIEFNPSVELQVLRSAHLRVGGREQMFETINDLNDHVRGVVAKLWCKHLHRSLAAAPTFTEEGLLDSFLSKPPVDINPDTTFRELFGINPHEQFPLSIHDLAKLQGELVDAARNPGHVLRRHYSTDSLTALINKITKFVPVHATLQEMQARRAFERERAELFRELPHADLDVSRQQKSYFYAMWRQVVKKSKEFFIPLVKCTSWRKKFTEPAEIVRQVLVHFCEGMRSQFSTNANYINLSLIAKLRPTVLTMILQQHKNTYRGWLATVTALVEVYSNLFQDMRDTAVSAVSAITLVFETIKDFVVNVIDLVKSTFQSQGPTSCGWAAIIAGALLILMKLSGCSNTTSYWHRLLKVCGGVTTIAAAARAVVWVRDIIAEADGKARLKKYMARTAALLELAASRDVTGTDELKRLLDCFTQLIEEGTELIQEFGTSPLAGLTRSYVSELESTANSIRSTILLDTPRKTPVAIILTGPPGIGKTRLAQHLAAGFGKVSNFSVTLDHHDSYTGNEVAIWDEFDVDTQGKFVETMIGVVNTAPYPLNCDRVENKGKVFTSDYIICTSNYPTSVLPDNPRAGAFYRRVTTIDVSSPTIEDWKKKNPGKKPPPDLYKNDFTHLRLSVRPFLGYNPEGDTLDGVRVKPVLTSVDGLSRLMETKFKEQGNEHRNLWITCPRDLVAPAASGLKAYMAANRALAQVFQEPSSQDIGETCTSRVYVSCNNPPPTYSGRVVKITAINPWDASLANSMLSMFETTSHIPASIQRE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm .; Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins; Attaches virion to target cells, inducing endocytosis of the viral particle.2. The For 3CLpro activity active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. A protein that can perform RNA helicase activity, ribonucleoside triphosphate phosphatase activity, RNA-dependent RNA polymerase activity, RNA binding in viral capsid, host cell cytoplasm to support viral RNA genome replication, DNA-templated transcription, proteolysis.4. The designed protein should have a binding site that is specific to the target ATP ligand.5. The designed protein have SF3 helicase domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "IVPVDQYMCGADGKPVLQESEWEYTDFFADSEDGQLNIAGITYVKAWIVERSDVSYASQNLTSIKSITYCSTYEHTFLDGTAMKVARTPKIKKNVVLSEPLATIYREIGSPFVDNGSDARSIIRRPVFLHAFVKCKCGSYHWTVGDWTSYVSTCCGFKCKPVLVASCSAMPGSVVVTRAGAGTGVKYYNNMFLRHVADIDGLAFWRILKVQSKDDLACSGKFLEHHEEGFTDPCYFLNDSSLATKLKFDILSQ",
    "response#2": "IVPVDQYMCGADGKPVLQESEWEYTDFFADSEDGQLNIAGITYVKAWIVERSDVSYASQNLTSIKSITYCSTYEHTFLDGTAMKVARTPKIKKNVVLSEPLATIYREIGSPFVDNGSDARSIIRRPVFLHAFVKCKCGSYHWTVGDWTSYVSTCCGFKCKPVLVASCSAMPGSVVVTRAGAGTGVKYYNNMFLRHVADIDGLAFTMVGEYMVLAPHIVLSCAKFDCIHELAEQVTTITHKGRIGRYAVEPFDARVLVPSGHAIPIPEFQAMSESATFTWNERTMINRRLHHIAVKGAAINTDEEQYKVMKAKDCKQEYMYDVDTRACIPTSAVTGFGLVGDLVNPPYHEFAFEGLKSRPAAGYAIHIGHGVYISLKHVVSGNARILSEEPKNLTFTGELATFRLNNILPTAVPVGTSKPIKDPWGNPVSTDWQFKNYNTTSGNIYGACGSSCSLTRQGDCGLPYVGGSV",
    "response#3": "MMELAEIDTMIPFDLSATKKNTMEMYRVQLSDKPHTDDPILCLSLSPASDPRLSHTMLGEILNYYTHWAGSLKFTFLFCGSMMATGKLLVSYAPPGADPPKKRKEAMLGTHVIWDIGLQSSCTMVVPWISNTTYRQTIDDSFTEGGYISVFYQTRIVVPLSTPREMDILGFVSACNDFSVRLLRDTL"
  },
  {
    "reference": "MFLNGISLRLTRRSGMWHLLLNMGPNSISIPLDSSSSIRRRPRWYSVRRHNQVLILHLVASSLHWLITIFPNTQQILCQTLPSLSIVSQDI",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be able to modulate viral process in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "MVFLQGVAGIGKSLMLTKLMLAWSEGMVFQNKFSYIFYFCCQDVKKMKRASLAELISKEWPNASAPIEEILSQPEKLLFVIDNLEVMECDMSEWESELCDDCMEKQPVNILMSSLLRRKMLPESSFLVSATPETFEKIEDRIECTNVKMMAGFNESSIKVYFYSLFQR",
    "response#2": "MVFLQGVAGIGKSLMLTKLMLAWSEGMVFQNKFSYIFYFCCQDVKKMKRASLAELISKEWPNASAPIEEILSQPEKLLFVIDNLEVMECDMSEWESELCDDCMEKQPVNILMSSLLRRKMLPESSFLVSATPETFEKIEDRIECTNVKMMAGFNESSIKVYFYSLFQH",
    "response#3": "MVFLQGVAGIGKSLMLTKLMLAWSEGMVFQNKFSYIFYFCCQDVKKMKRASLAELISKEWPNASAPIEEILSQPEKLLFVIDNLEVMECDMSEWESELCDDCMEKQPVNILMSSLLRRKMLPESSFLVSATPETFEKIEDRIECTNVKMMAGFNESSIKVYFYSLFQR"
  },
  {
    "reference": "MNTLECLAVAVALAMDAFAVAIATGIRLREVSPRQTFRLAFHFGLFQALMPVAGWTLGLTVRGYIEQWDHWLAFGLLLYIGVRMMREAFEETEENDDRCDPTRGLTLIMLAVATSIDALAVGLSLSVLGIDIVTPAIVIGVVCLLFTATGLHLGRMLSRAESLGRRAALAGGVVLIGIGLRILYEHGVFDTAATLARSVLG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "LHHHHHH",
    "response#2": "SQI",
    "response#3": "GTLSS"
  },
  {
    "reference": "MSLMILSVDGVEFAYKSRQILNNIKFEVKRGEVVSILGVNGAGKSTLLKCINKILKPKRGTILIDNFDIKNLDNLELAKKVGYVPQRAEGNYMTVFDAVLLGRKPHIKWEVSDRDIEITHKVLKLLNLEDYALRYTNELSGGELQKVIIARALVQEPQILLLDEPTNNLDLKNQLEVMKIIMDISKSQNIASIVVMHDLNLALRYSDKFIMLKDGVIYAEGGREVINPENIKAVYGVDAYIENVRGIPVVVPIG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must be able to perform ATP hydrolysis activity, ATP binding, ATPase-coupled transmembrane transporter activity under specific conditions.2. The protein should be able to bind ATP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "RGGKIGALGGAGVGKTVLIQELIRNIATEHEGFSVFAGVGERSREGNDLWHEMEDSGVLPKTTMVFGQMNELPAVRLRIALTGLTMAEYFRDEERQDVLLFIDNIYRYTLAGMEVSALLGRMPSAVGYQPTLATEMGALQERIASTKQGSITSFQAVYVPADDYTDPGVVATFGHLDAMIALERSDQQYPAVDPLASNGIKVVDLLAPYSKGGKVGLFGGAGVGKTVIIMELINNVAMKHSGYSVFAGVGERTREGNDLYYEMKESNVLDKVALCYGQMSEPPGARNRIALTGLTMAEYFRDEMGLDVLMFIDNIFRFAQSGSEMSALLGRIPSAVGYQPTLSREMGALQERITSTTKGSITSVQAVYVPADDLTDPAPASVFAHLDATTVLNRSIAEKGIYPAVDPLDSTL",
    "response#2": "KGGKVGLFGGAGVGKTVIIMELINNVAMKHSGYSVFAGVGERTREGNDLYYEMKESNVLDKVALCYGQMSEPPGARNRIALTGLTMAEYFRDEMGLDVLMFIDNIFRFAQSGSEMSALLGRIPSAVGYQPTLSREMGALQERITSTTKGSITSVQAVYVPADDLTDPAPASVFAHLDAS",
    "response#3": "RGGKIGALGGAGVGKTVLIQELIRNIATEHEGFSVFAGVGERSREGNDLWHEMEDSGVLPKTTMVFGQMNELPAVRLRIALTGLTMAEYFRDEERQDVLLFIDNIYRYTLAGMEVSALLGRMPSAVGYQPTLATEMGALQERIASTKQGSITSFQAVYVPADDYTDPGVVATFGHLDAMIALERSDRGGKTGLFGGAGVGKTVLIMELINNIAKQQSGYSVFAGVGERTREGNDLWHEMMESGVIDKTALVFGQMNEPPGARARVALTGLSIAEYFRDEEHRDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGELQDRITSTKNGSVTSVQAIYVPADDLTDPAPATAFAHLDATTVLSRQIAELGIYPAVDPLDSTSRILDPNVIGDDHYDTAQSVKQILQRYKDLQDIIAILGMDELSDEDKLVVARARKVQRFLSQPH"
  },
  {
    "reference": "MEAVQLDKNQEPNYKGYSGSLIHPAFQQQTTKREKPSTPLPSLDLLLKYPPNEQRITPDEIMETSQRIEQQLRNFNVKASVKDVLVGPVVTRYELELQPGVKASKVTSIDTDLARALMFRSIRVAEVIPGKPYIGIETPNLHRQMVPLRDVLDSNEFRDSKATLPIALGKDISGKPVIVDLAKMPHLLVAGSTGSGKSVGVNTMILSLLYRVQPEDVKFIMIDPKVVELSVYNDIPHLLTPVVTDMKKAANALRWCVDEMERRYQLLSALRVRNIEGFNEKIDEYEAMGMPVPNPIWRLGDTMDAMPPALKKLSYIVVIVDEFADLMMVAGKQIEELIARLAQKARAIGIHLILATQRPSVDVITGLIKANIPSRIAFTVASKIDSRTILDQGGAEALLGRGDMLYSGQGSSDLIRVHGAYMSDDEVINIADDWRARGKPDYIDGILESADDEESSEKGISSGGELDPLFDEVMDFVINTGTTSVSSIQRKFSVGFNRAARIMDQMEEQGIVSPMQNGKREILSHRPEY",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that May function as a DNA motor that moves dsDNA in an ATP- dependent manner towards the dif recombination site, which is located within the replication terminus region; Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination2. The protein contains novel FtsK domains that confer a unique function or activity.3. The designed protein should have ATP binding, DNA binding to facilitate cell division. The designed protein sequence is ",
    "response#1": "RLKECCLILGVLTSAFLSIALLTFSPADPSWSQTSWGGDISNAGGQFGAWVADTLFFTFGLLAYLLPVLLVIVTWVFFRTRDEDEHIDLMLWGTRLLGLAILILTSCGLADINFDDIWYFSSGGVLGDVLNSLALPTLNLLGTTLVLLFAWGAGFTLLTGISWLSIVEWIL",
    "response#2": "VQAVILGIIGLATISMLVSYKLDDPSFNSATTGYTNNLLGIFGSYLSDFLYQFFGVAAFIIPLSCFIWGKNCWQQKYRKSFIRISVMLLALFSTAALLSNFDLEFVPSNGGGAAGIIIFHFLKQFTNQLHLLLVFFTFIIFVVLFEIKFTAL",
    "response#3": "RLKECCLILGVLTSAFLSIALLTFSPADPSWSQTSWGGDISNAGGQFGAWVADTLFFTFGLLAYLLPVLLVIVTWVFFRTRDEDEHIDLMLWGTRLLGLAILILTSCGLADINFDDIWYFSSGGVLGDVLNSLALPTLNLLGTTLVLLFAWGAGFTLLTGISWLSIVEWITK"
  },
  {
    "reference": "MRILVAMSGGVDSSVVAALLKRQGHEVIGATLQLYDHGQAAKPGACCAGRDIMDARAVADRLGFPHYVIDAESRFRDSVVESFADAYARGETPVPCVACNQGVKFTDLLGMARDLGCEAMATGHYVRRVEGPEGAEMHRPVDAERDQTWFLFATTKDQLDYLRFPLGEMPDKAHVRALAQELGLEIAAKPDSQDICFVPSGSYAELVEKLRPEVRGEGEIVDEDGRVLGRHEGVARYTVGQSKRLGDIHTATGERQMVTRIDVPKRRIVVGPRVQVSEADSRRSVRLRDMNWLIDAPAEGVRCGVQIRAREKLREALVKPLENGGALVELTEAAMPAPGQACVLYDGSRVLGGGFITAGDA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein have enhanced AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in   tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-   cysteinyl-[protein].2. The protein must exhibit the following characteristics: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34..3. The protein should be able to bind ATP ligand in a variety of conditions4. A protein that contributes to tRNA modification by functioning in cytoplasm with tRNA binding, ATP binding. The designed protein sequence is ",
    "response#1": "FEAEVLHTVLAGHLLPPGAVVAVGASGGKDSTVLAHVLRELAPRLGITLHLVAVDEGIGGYRDAALEAVRSQAARWELPLTIVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARLVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGVLGSTGEGCALPRCRPLQFASQKEVVLYAHFRHLRYFSEECVYAPEHSRLEVRPHITVAAVIEREGKXLLVEEISDGLIVYNQPAGHLEPNESILAAISRETLEETGWEFSPKSIVGIYFYQSELNQITYQRICFAGKLGKQTEHPLDVGIIRSVWLSRDEIIQQALSEGVRAWVP",
    "response#2": "FEAEVLHTVLAGHLLPPGAVVAVGASGGKDSTVLAHVLRELAPRLGITLHLVAVDEGIGGYRDAALEAVRSQAARWELPLTIVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARLVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGVLGSTGEGCALPRCRPLQFASQKEVVLYAHFRHLRYFSEECVYAPEFEAEVLHTVLAGHLLPPGAVVAVGASGGKDSTVLAHVLRELAPRLGITLHLVAVDEGIGGYRDAALEAVRSQAARWELPLTIVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARLVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGVLGSTGEGCALPRCRPLQFASQKEVVLYAHFRHLRYFSEECVYAPEEGRHLPHITVAAVIEREGKXLLVEEISDGLIVYNQPAGHLEPNESILAAISRETLEETGWEFSPKSIVGIYFYQSELNQITYQRICFAGKLGKQTEHPLDVGIIRSVWLSRDEIIQQARERDGRP",
    "response#3": "FEAEVLHTVLAGHLLPPGAVVAVGASGGKDSTVLAHVLRELAPRLGITLHLVAVDEGIGGYRDAALEAVRSQAARWELPLTIVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARLVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGVLGSTGEGCALPRCRPLQFASQKEVVLYAHFRHLRYFSEECVYAPEVRRHEFFTPHITVAAVIEREGKXLLVEEISDGLIVYNQPAGHLEPNESILAAISRETLEETGWEFSPKSIVGIYFYQSELNQITYQRICFAGKLGKQTEHPLDVGIIRSVWLSRDEIIQQALGQD"
  },
  {
    "reference": "MKIAVVGATGMVGEVMLQVLRERNFPVSELLLVASEKSVGKKIFFLDKEYQVIGLAEAVLAKPDIALFSAGGETSLIWAPKFAAVGTTVIDNSSAWRMDASKKLVVPEINASTLTLKDKIIANPNCSTIQLVMTLAPLHKKYGIKRVVISTYQSITGTGVKAVKQLENEYKNIKGEMAYSYPIHQNAIPQCDAFEENGYTKEEMKLVRETQKILDDKTIAVTATAIRIPVVGGHSESVNIEFLKDFDVSEIRNILHKTPGIVVQDNPELSTYPMPIYAKGKDDVFVGRIRRDASQPNTLNIWIVSDNLRKGAATNAVQIAEYLVSNHKFLKNRVTL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be optimized for protein dimerization activity activity in order to improve lysine biosynthetic process via diaminopimelate. The designed protein sequence is ",
    "response#1": "MRVLKFGGTSVANDNKMLNVANIVADKFTAGPVALVLSAPAKITDYLIAMIDAATSGADITPHIKGANEIFSALISGLQEKQSDFAYEKVRQMIENEFIQIQQILQGIELLGLGQCPASLYAGLICRGEKMSITIMASILRARGYLVTIIDPVKNLFAEGPYLGATVDIRESSQRISALNIPKDHLILLAGFTAGNGQNEPVILGRNGSDYSADVLAACLRADYCEIWTDVAGVYTCDPRIVPEAQLLTEISYQEAMELSYFGAKVLHPRTIAPIAQFQIPCLIKNSSAPEGKGTLIGNIENSECMPIKGITSLNNMAMINVSGPGMKGMVGMAARVFSALSQKGISIILITQSSSEYSISFCVYQEQLSQSYQALNEEFYLELKEGLLGSLDIIEQLAIISVVGDGMRTCKGISARFFAALTRGNINIVAIAQGSSERSISAVIDNNMSTIAVQLCHQMLFNTDQIINVFVVGVVGNALLEQIYRQQNWLKKQQIDLRVCAIANSRVMLTDITGLTLDNWQIALSQSKRSFSLRDVCQLQDKHCFLNPVIVDCTADQQIAKHYVFLLKAGFNIVTPNKKANTDSMHYYRQIRLVAEQSKRKFLYEANVGAGLPVIENLQNLLNAGDELLAFSGILSGSLSYIFGLLDEGMSLSQATQLAKEKGFTEPDPRDDLSGMDVARKLLILAREVGYCLELSDIEIEPILPISFDTSGNVNHFLQRLTQLDQDFKIRVAQAAAQGKVLRYVGLIKDGRCQVKVVAVDEHNPLH",
    "response#2": "PVIENLQNLLNAGDELLAFSGILSGSLSYIFGLLDEGMSLSQATQLAKEKGFTEPDPRDDLSGMDVARKLLILAREVGYCLELSDIEIEPILPISFDTSGNVNHFLQRLTQLDQDFKIRVAQAAAQGKVLRYVGLIKDGRCQVKVVAVDEHNPLN",
    "response#3": "LAGLFNNDLIEWMTSMTYQAASGGGARHMRELLVQFGLLNDAVDDLLADPASAILEIDRGVLARQKTAELPHDQFGVPLAGSLIPWIDSDLGNGVSREEWKGEAETNKILGRGPAFGTASTPVDGLCVRIGAMRCHSQALTIKLKKDVGLDEIADMLREGSRWARIVPNEREASMRDLTPVAVTGTLDVPVGRLRKMSMGPEYLSAFTVGDQLLG"
  },
  {
    "reference": "MEKYRFNSIEEALDDIAAGKMVILVDDEDRENEGDLCMAAEKVTPEAINFMAKYGRGLICLSLTPDRVEKLKLPMMTDDNTSPFGTAFTVSIEAKKGVTTGISAADRARTILTAIDPGAKPEDLTRPGHVFPLKAKAGGVLQRAGQTEGSVDLARLAHLSPAGVICEIMSDDGTMARVPELMEFSKRHNLKIVMIKDIIKYRMRIESFVKRLAQVKLPTIYGGDFTAIAYENDIDTNVHVALVKGDIKPEDEVLVRVHSQCLTGDVFASQRCDCGEQLHKAMEMIRKEGKGVILYMKQEGRGIGLINKLRAYELQDKGLDTVEANIKLGFKPDLRDYGIGAQILVDLGVRKMRLMTNNPKKIVGLEGYGLQVVARVPIEMSPNERNILYLKTKKKKLGHMLDNV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..2. The designed protein should be optimized for magnesium ion binding, GTP cyclohydrolase II activity, manganese ion binding, GTP binding activity in order to improve riboflavin biosynthetic process.3. The protein design should consider the endogenous proteins and enzymes present in the riboflavin biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4, Cofactor biosynthesis pathway to avoid interference with native metabolic processes.4. The Nucleophile; for GTP cyclohydrolase activity of the protein must be engineered to enable effective catalysis of GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.5. The designed protein should contain one or more GTP cyclohydrolase II domains that are essential for its function.6. The designed protein should have a binding site that is specific to the target Zn(2+), D-ribulose 5-phosphate, Mg(2+), GTP ligand.7. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "RMVSTVLPTRNGRFDVHLYRSAVDTKEHIALVKGTIDAGAPVLVRVHSECLTGDIFGSQRCDCNEQLHTSMEMIEREGNGIVLYMRQEGRGIGLLNKLRAYKLQDEGKDTVEANEALGFHADVRDYSLAAQILLDLGVRRVRLLTNNPKKIVGLKGFGIEVTERIPHPEVAERVADAKLPTRYGDFVTVAYSSVVDAEEHIALVMGDIDPEVPTLVRVHSQCTTGDIFGSLRCDCGPQMDMALEAIAKEGKGVFLYMRQEGRGIGLHNKIRAYALQDKGMDTVEANVELGFAPDLRWYGIGAQILADLGVKKLRLLTNNPKKVVGLEAYGLELVDRVRAAEARLPTEHAGDFTAIVYTNDVDQHHHIALVKGDISKADRVLVRVHSECLTGDVFGSSRCDCGVQLSAAMRMIDQEGCGVLVYMRQEGRGIGLINKLKAYYLQDSQGMDTVEANLKLGFKADLRDYGIGAQILRDLGVKKMALLTNNPKKIIGLEGYGIEVVARYREVEVRMPTEFGEFTMYGYSNRVDAAEHVALVMGKPGPEKPVLVRVHSECLTGDVFTSRRCDCGSQLHAAMRQIAEEGEGVILYLRQEGRGIGLLNKLRAYKLQDEGHDTVEANRKLGFKADLRDYGIGAQILRDIGLRKLRLLTNNPKKIIALDGYGLEVVERG",
    "response#2": "MSERVTQARIPTVSGAFKAIAYESHDGRTHMALVAGDPAGKEHVLVRVHSECFTGDVLGSTRCDCGVQLSEALERIQQEGEGVLVYIRGHEGRGIGLRHKVEAYALQDGGLDTVEANVELGFAPDTRDYGVGAQILRDLGITTMRLLTNNPTKRAGLEGYGLEIVERIPLPRSPKERIAETRLPTRYGEFTAFGYRMSIDGSEHIALVHGDPADLKDGEPVLTRVHSECLTGDIFGSQRCDCGPQLEEALERVATEGRGVVIYLRGHEGRGIGLLAKLQAYALQDAGRDTVDANLDLGLPADARHYGAATQILRDLGVGSVRLMTNNPAKVTGLSNYGVDVSERVERVTQARIPTVSGAFKAIAYESHDGRTHMALVAGDPAGKEHVLVRVHSECFTGDVLGSTRCDCGVQLSEALERIQQEGEGVLVYIRGHEGRGIGLRHKVEAYALQDGGLDTVEANVELGFAPDTRDYGVGAQILRDLGITTMRLLTNNPTKRAGLEGYGLEIVERIPLARLVG",
    "response#3": "MNDREIHNCARLLEALDATAVRGDLKVTVALLSDSRQVAPGDVFVAHQGAMTDGGRFIADALARGAAGVISEEPVPEGPVTQIVVPDTRAALSLAAARWFGDPARQLDVLA"
  },
  {
    "reference": "MKLLDAESMRELDRRTIEKTGIPGIVLMENASRRVFEAIEAIVGELEGKRALVICGKGNNGGDGFAVARYLMNAGVDTTAALAAARKEVKGDAAVNMSVFLNMGGELKLTTDAKGLTDLRRAAAASDFVVDALLGTGVKGPVSAPYKKVIEIINKSGCPVFAVDAPSGVSVDDGRVFNCAVNADHTVTFGEAKIGLFVWPGAEKAGEVYVADIGIPASHSRETRSKVYLTQPGYVREHVKERPMGAHKGDCGKLLIVGGSRGMSGAPALAGMAALKIGAGLVYLAVPKSVASNVERKLTEGITISLREDGRGRISASELEEIIARSAAAGAVALGPGLGVSPDTRKLTFKMIESVSVPLLLDADALNCVAVDPEILKTAAGPVVVTPHPGEMARLAKAKTADVQAARLDTARSFAAEYGVTVVLKGAGAIVASPDGCVRVNPTGNPGMATAGSGDVLTGMTGALMAEGVDVFAAAACAAYLHGLAGDLAAEKLTRRAVTASDIVRFAPAALNETLSEEC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The desired protein should be able to perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate2. The protein design should be able to enhance the efficiency of nicotinamide nucleotide metabolic process.3. The protein should be able to bind (6S)-NADPHX, AMP, K(+) ligand in a variety of conditions4. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.5. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration The designed protein sequence is ",
    "response#1": "MRALDVNAEYFGISRLQLMENAGGSVAAEIALRFKPKETRVAVFCGLGGNGGDGFVAARHLACLGFNVNVILAGKSSEISVEESRRNWAALKHLRRSVTIYEVHDSSLIPNIEADVIVDALLGIGLKAPPRPPIAQLIRAINDSDAFRVAVDVPSGLDSDTGAVLGEAVKANLTVTFHKAKPGLLKAKEYVGELIVRQIGIPEEIEGPGDVITSLKRRFFENRKGDFGKLLVIGGSDKYHGAPILSAMSSIRAGVDLVIISTPELIANTIRAHTPNFIVRDYPGRYLTLDSVKYISDLINWADAIVLGPGLGVESETKEAVCKLFTLIKEANKPLVVDADALKVLAENPRILAGSPAVATPHRGEFKLLTGLEIKSSADILDNINEILSKISEFDITFAVKGAVDLVAYKNMFKLDLLGNPGMTVGGIGDVLTGVLGCFLSQGVEPFKAACAAVFITGLSGNLIAEKKGYHFTSAELIEYIPEALEEHALLERLVLCVGGDTGHGGAVMLAADAALRCGAGLASVATRSAHVPGLLARRPEAMARGVDGAAALAPLLARADVVALGPGLGQGSWGRALYGAVLAAGLPLVLDADALNLLAAAPRELAADTILTPHPGEAARLLGMETTQVQADRFAAAARLVERYGCVVVLKGAGTVVAAPGQSPRVVAAGNPGMAVGGMGDLLTGCIAALRARGMPAFEAASLGALLHALAGDAAARDGETGLLPSDLIL",
    "response#2": "MKNQAYDLDRLALKTYGISGQKLMGEAGKKIADFIKSNFSNMNLISIVVGKGNNGGDGFAAALYLNKCGFNIAVFSVYDSQYFSTETSYYYKECSKEGISVIFASDPPSAYDFNCLVIDSMLGIGFRGELFENLKNWSTWINRFSVVVSCDIPTGVCSDSGTVSTEAVKADYTIAIGYPKIGSLIEPGKSYSGTITSIDIGFLIVAGSSGKTGAAALSANSAVRAGSGLVTLAVPESLNPILEVKTTEAMTVPLADSGGGYLTDSAFPAIAGLLPDKDALAIGPGLGQHPATVTLVRALLKAADLPLVIDADGLNAVAQNANCLLDKKSTHVVLTPHPGEMARLMGATIADVEADRIATARKFAAAFGVHLILKGSRTIIAAPDGSTAINGSGNPGMASGGMGDVLTGIIVSLLGQGYNCHDACRLGVFIHGYAADLVAQDKGEIGMCAGDVQEKLAYAHKGDAGRAVICAGSPGFTGAAALSSYAAVKAGAGLVALYTPLSSRDVLAGKLTEVMVHGLLERMPGILGGGATGDVVKNANAADVLAIGPGLGTSESTQQVVRDILLQVETPVVIDADALTALQGHTEILTQMQAAKVLTPHPGEMARLIGKEISEINADRINIAAQYAQEWNAVLVLKGAPTVIACPDGTVYVNSTGSSALATGGSGDVLTGIIAGLAAQEITLQEAAVCGVYLHGKAADVAVIGGARGMSGAPILAARTAINSGVGRVYALFVEDALPCDPGQPELMCRSAGDFELNTATLVIGPGLGDSSAAHALLDQAIASDSVLLADADAINLLAGDAALQTRLAQRQAAVVLTPHPLEAARLLGSSIGAVQADRLAAARTLAARLQAIVVLKGSGTVIAAPDGKAVINTTGNPALATAGTGDVLAGLCGALLAQGWPQWEAALAAVWLHGMAADVLVT",
    "response#3": "MKNQAYDLDRLALKTYGISGQKLMGEAGKKIADFIKSNFSNMNLISIVVGKGNNGGDGFAAALYLNKCGFNIAVFSVYDSQYFSTETSYYYKECSKEGISVIFASDPPSAYDFNCLVIDSMLGIGFRGELFENLKNWSTWINRFSVVVSCDIPTGVCSDSGTVSTEAVKADYTIAIGYPKIGSLIEPGKSYSGTITSIDIGFPKSLPPEAKLIAYAHKGDAGRAVICAGSPGFTGAAALSSYAAVKAGAGLVALYTPLSSRDVLAGKLTEVMVHGLLERMPGILGGGATGDVVKNANAADVLAIGPGLGTSESTQQVVRDILLQVETPVVIDADALTALQGHTEILTQMQAAKVLTPHPGEMARLIGKEISEINADRINIAAQYAQEWNAVLVLKGAPTVIACPDGTVYVNSTGSSALATGGSGDVLTGIIAGLAAQEITLQEAAVCGVYLHGKAADRTLAQRFLPLPRPLLTHKYKEGHLLVICGSYRYAGGAILTGLGARGSGVGMLSIAVPKSLKSMLVNHLPDALIIDCPETPSGAIAELPPLASEFDNYSVIACGPGLTSEAKSIVETVLEAKCPLVLDADGLNNLAQIDPISSLLKRPAPTILTPHIGEFKRLFPNIENPHQDRLKAVQEAAQQSGSIVLLKGARTAIAAPNGCVWLVPESTPALARGGSGDVLTGLIGGLIAQQMTSEQAMERTVATAAWWHSQAGILAAQERTELGVDAFTLSQYLWKL"
  },
  {
    "reference": "MSDSSATGFSKHQESAIVSDSEGDAIDSELHMSANPPLLRRSSSLFSLSSKDDLPKPDSKEYLKFIDDNRHFSMIRNLHMADFITLLNGFSGFYSIISCLRYTLTGQTHYVQRAHFFILLGLFFDFFDGRVARLRNKSSLMGQELDSLADLVSFGVSPATIAFAIGFRTTVDVLFLAFWVLCGLTRLARFNISVNNIPKDKHGKSQYFEGLPIPTNLFWVGFMALLVYKDWIHDNLPFGIVFQDTSFEFHLVTIGFVLQGCAEISKSLKIPKP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization.2. The protein should have a specific function in filamentous growth of a population of unicellular organisms in response to chemical stimulus, plasma membrane organization, phosphatidylserine biosynthetic process, filamentous growth and should be localized to endoplasmic reticulum membrane in order to carry out this function efficiently.3. Ideal protein should have a binding affinity for key enzymes in the phosphatidylethanolamine from CDP-diacylglycerol: step 1/2, Phospholipid metabolism, phosphatidylethanolamine biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MTPSQFGLVSSIYTLGGLLGALLAGPIATRYGRLVALRATTIFFILGPIAETLAPAISVLGLGRFLSGIGSGAAIVVGPIYISEIAPPSARGLFGAFTQIMTNVGILWTQSLGYFWSEASLWRLILAVAGIVGALELLGLFLVPESPPWLAEHQKISQAHRVLQRIRGRDADIEAEIASWRTTDAPVSGEEESLLSPSGDIPSNKPAVTFMQVVKDPYYRPAIIAVVGVMVAQQLTGMNSIVMYSVSLLQSILPTTAALLTVMISAVNLVITLVCSPLPDRIGRKLCLMLSICGMGCSSILLAISIYMNAKILTAIAALLFVACFAVGLGPVPFILASELVGPEAVGAAQSWALGANWIATFVVAQFFPVLDDALGGRGQVYWVFAAMAGLLGVFIYQYVPETKNRTYEEITGE",
    "response#2": "MTPSQFGLVSSIYTLGGLLGALLAGPIATRYGRLVALRATTIFFILGPIAETLAPAISVLGLGRFLSGIGSGAAIVVGPIYISEIAPPSARGLFGAFTQIMTNVGILWTQSLGYFWSEASLWRLILAVAGIVGALELLGLFLVPESPPWLAEHQKISQAHRVLQRIRGRDADIEAEIASWRTTDAPVSGEEESLLSPSGDIPSNKPAVTFMQVVKDPYYRPAIIAVVGVMVAQQLTGMNSIVMYSVSLLQSILPTTAALLTVMISAVNLVITLVCSPLPDRIGRKLCLMLSICGMGCSSILLAISIYMNAKILTAIAALLFVACFAVGLGPVPFILASELVGPEAVGAAQSWALGANWIATFVVAQFFPVLDDALGGRGQVYWVFAAMAGLLGVFIYQYVPETKNRTYAEISGE",
    "response#3": "MTPSQFGLVSSIYTLGGLLGALLAGPIATRYGRLVALRATTIFFILGPIAETLAPAISVLGLGRFLSGIGSGAAIVVGPIYISEIAPPSARGLFGAFTQIMTNVGILWTQSLGYFWSEASLWRLILAVAGIVGALELLGLFLVPESPPWLAEHQKISQAHRVLQRIRGRDADIEAEIASWRTTDAPVSGEEESLLSPSGDIPSNKPAVTFMQVVKDPYYRPAIIAVVGVMVAQQLTGMNSIVMYSVSLLQSILPTTAALLTVMISAVNLVITLVCSPLPDRIGRKLCLMLSICGMGCSSILLAISIYMNAKILTAIAALLFVACFAVGLGPVPFILASELVGPEAVGAAQSWALGANWIATFVVAQFFPVLDDALGGRGQVYWVFAAMAGLLGVFIYQYVPETKNQSFEEITGE"
  },
  {
    "reference": "MSHKNIWNKHSRTYGKGSRGCRICGNRSGLIRKYGLSICRQCFREKSQNLGFDKYR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be optimized for ribosome, ribonucleoprotein complex-specific folding and stability, while maintaining structural constituent of ribosome.2. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "MTRGNRICFGRFALQALEPAWITSGQIEAGRRTINRYARRGGKIWVRIFPDKPITMRPAETRMGSGKGSPEYWVSVIRPGRILYEMGGVSETVARAAARIAAYKMPIRTQFS",
    "response#2": "MF",
    "response#3": "LRGNRICFGRFALQALEPAWITSGQIEAGRRTINRYARRGGKIWVRIFPDKPITMRPAETRMGSGKGSPEYWVSVIRPGRILYEMGGVSETVARAAARIAAYKMPIRTQFR"
  },
  {
    "reference": "MYPTRPHYWRRRLSINRPQAFLLLILCLFFIHHCDASRFSSSSVFYRNPNYDHSNNTVRRGHFLGFLPRHLPVPASAPSRKHNDIGIQALLSP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be able to modulate floral organ abscission in a way that leads to a desirable outcome.2. For general function, the protein need meet that May be involved in floral abscission. The designed protein sequence is ",
    "response#1": "VGTTASLSLRLGQMVLAFGSLLFMTIGVRFYQFTAFCYLVTIMSLAIPWNLTLAMVDIYCVILQQPFQKPRILLAISIGDWVVSVLALASASSAASVVDILRSNESSCPPTICNRYQFAATLAFLTWFN",
    "response#2": "VGTTASLSLRLGQMVLAFGSLLFMTIGVRFYQFTAFCYLVTIMSLAIPWNLTLAMVDIYCVILQQPFQKPRILLAISIGDWVVSVLALASASSAASVVDILRSNESSCPPTICNRYQFAATLAFLTWFR",
    "response#3": "VGTTASLSLRLGQMVLAFGSLLFMTIGVRFYQFTAFCYLVTIMSLAIPWNLTLAMVDIYCVILQQPFQKPRILLAISIGDWVVSVLALASASSAASVVDILRSNESSCPPTICNRYQFAATLAFLTWFR"
  },
  {
    "reference": "MEALSINAPTQASTLPSGLQISKEVEKRYNVVRSVGEQCIHDDELKDLLAKKAAPVCYDGFEPSGRMHIAQGLMKIMNVNKLTSAGCRVKIWIADWFAYMNNKLGGDLKKIRVVGEYFKEIFQAAGMNSENVEFLWSSDEINAKGDEYWPLVMDIACRNSLAQIKRCMPIMGLSENEELSAAHILYVCMQCADTFFLEADICQLGMDQQTVNLLARDYCDVVKRENKPVILSHHMLPGLQQGQKKMSKSDPSSAIFMEDEEAEVNVKIKKAYCPPDIVEGNPCLEYVKHIILPWFSEFTVERDEKYGGNRTFKSFEDITTDYESGQLHPKDLKDALSKALNKILQPVRDHFKTNSRAKNLLKQVKGYKVTRVIPTASSTKEEDLSINTSASSSAAGLQMSEEAEMKYKIVRSIGEECIQEDELKNLLAKKPAPICYDGFEPSGRMHIAQGVMKVTNVNKLTSAGCQVKIWIADWFAQLNNKLGGDLERIRVVGEYFKEIWQAGGMNNDKVEFLWASDEINGKGSKYWPLVMDIARRNNLRRILRCGQIMGRSETEVLSAAQILYPCMQCADIFLLEADICQLGMDQRKVNMLAREYCADIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSAIFMEDEEADVNEKISKAYCPPKTVEGNPCLEYVKYIVLPRFNEFKVESEKNGGNKTFNSFEDIVADYETGELHPEDLKKALMKALNITLQPVRDHFKTNERAKNLLEQVKAFRVTR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should contain at least one 'HIGH' region, 'KMSKS' region motif to achieve its intended function.2. The protein should be designed to have high stability and activity towards ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + H(+) + L-   tyrosyl-tRNA(Tyr).3. The designed protein should be able to compete with endogenous proteins or inhibitors for L-tyrosine binding.4. The protein must exhibit the following characteristics: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two- step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr)..5. The designed protein should have tyrosine-tRNA ligase activity to facilitate tRNA aminoacylation for protein translation. The designed protein sequence is ",
    "response#1": "MPTANLSLSKLNEGIGIIDLFAEAGLASTKSDARRLVEQGGAFINEEKISDIKALIGKEKLDKDNEMILRAGKKRFLWQELKQGLSNVPNYAVKSNDNHNIVELLVTAGIVNSKRQAREDLQNGAIYINGERIQDLTYNLSQQDKIDNELTVIRRGKKKYFNW",
    "response#2": "MPTANLSLSKLNEGIGIIDLFAEAGLASTKSDARRLVEQGGAFINEEKISDIKALIGKEKLDKDNEMILRAGKKRFVVMPTANLSLSKLNEGIGIIDLFAEAGLASTKSDARRLVEQGGAFINEEKISDIKALIGKEKLDKDNEMILRAGKKRFVVRV",
    "response#3": "MPTANLSLSKLNEGIGIIDLFAEAGLASTKSDARRLVEQGGAFINEEKISDIKALIGKEKLDKDNEMILRAGKKRFFS"
  },
  {
    "reference": "MGLIWLLLLSLLEPSWPTTGPGTRLRRDAGGRGGVYEHLGGAPRRRKLYCATKYHLQLHPSGRVNGSLENSAYSILEITAVEVGVVAIKGLFSGRYLAMNKRGRLYASDHYNAECEFVERIHELGYNTYASRLYRTGSSGPGAQRQPGAQRPWYVSVNGKGRPRRGFKTRRTQKSSLFLPRVLGHKDHEMVRLLQSSQPRAPGEGSQPRQRRQKKQSPGDHGKMETLSTRATPSTQLHTGGLAVA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The ideal protein would be one that plays a critical role in regulation of cell migration, fibroblast growth factor receptor signaling pathway, otic vesicle formation, cell differentiation through growth factor activity within cytoplasm, endoplasmic reticulum, extracellular space, Golgi apparatus.2. The protein must exhibit the following characteristics: Required for normal ear development .. The designed protein sequence is ",
    "response#1": "CWETEVGIEFVKLTVSDIQVTYLTILIGDFLRALIVRFLNYCWCWDLEAGFPSYAEFDISGNVLGLIFNQGMIWMGAFYAPGLVGINVLRLLSSMYYQCWAVMACNVPHERVFKAC",
    "response#2": "PQITVQPETQSAIKGSNLSFICSAASSSDSPMTFAWKKDNELLHDAEMENYAHLRAQGGEVMEYTTILRLREVEFASEGKYQCVISNHFGSSYSVKAKLI",
    "response#3": "CWETEVGIEFVKLTVSDIQVTYLTILIGDFLRALIVRFLNYCWCWDLEAGFPSYAEFDISGNVLGLIFNQGMIWMGAFYAPGLVGINVLRLLSSMYYQCWAVMACNVPHERVFKAC"
  },
  {
    "reference": "MSQLEKIYGVHAVQALLQHHPKRVKQIWLSEGRSEPRLETLLALAAENRVPVGQAERREMDAWVEGVHQGVVAEVSPSQVWGELMLEELLERTETPPLILVLDGVTDPHNLGACLRTADAAGATAVIVPKDKSATLTPVVRKVACGAAEVIPLVAVTNLARTLEKLQQRGLWVVGTAGEAEQEIYQQDLTGPLVMIMGAEGKGMRRLTREHCDFLVKLPMAGSVSSLNVSVATGVCLFEAVRQRQVKR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit RNA binding.2. The protein must exhibit the following characteristics: Specifically methylates the ribose of guanosine 2251 in 23S rRNA..3. The designed protein should have a high specificity for S-adenosyl-L-methionine binding, with minimal off-target effects.4. The protein should be designed to have high stability and activity towards guanosine(2251) in 23S rRNA + S-adenosyl-L-methionine = 2'-O-   methylguanosine(2251) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "REDGAYLYVNFTDYLDTGLFIDHRNMRARIKDNSRNKSVLNLFAYTCTASVHAALAGAKKVTSVDLSQNYLDWGKQNFALNGLNVSGNKYQFVAADIFEWIKDNTEQFDIIFIDPPTFSNSKKFQGTFDVQRDHAALINRAMNRLTADGQ",
    "response#2": "REDGAYLYVNFTDYLDTGLFIDHRNMRARIKDNSRNKSVLNLFAYTCTASVHAALAGAKKVTSVDLSQNYLDWGKQNFALNGLNVSGNKYQFVAADIFEWIKDNTEQFDIIFIDPPTFSNSKKFQGTFDVQRDHAALINRAMNRLTADGR",
    "response#3": "REDGAYLYVNFTDYLDTGLFIDHRNMRARIKDNSRNKSVLNLFAYTCTASVHAALAGAKKVTSVDLSQNYLDWGKQNFALNGLNVSGNKYQFVAADIFEWIKDNTEQFDIIFIDPPTFSNSKKFQGTFDVQRDHAALINRAMNRLTADGV"
  },
  {
    "reference": "MLVSYRWLGEYVDLTGITAKELAERITKSGIEVERVEALDRGMKGVVIGHVLECEPHPNADKLRKCLVDLGEDEPVQIICGAPNVAKGQKVAVAKVGAVLPGNFKIKRAKLRGEESNGMICSLQELGVETKVVPKEYADGIFVFPSDAPIGADAIEWLGLHDEVLELSLTPNRADCLSMIGVAYEVAAILGRDVKLPEAAVKENSEHVHEYISVRVEAPEDNPLYAGRIVKNVRIGPSPLWMQARLMAAGIRPHNNVVDITNYILLEYGQPLHAFDYDRLGSKEIVVRRAKAGETIITLDDVERKLTEEHLVITNGREPVALAGVMGGANSEVRDDTTTVFIEAAYFTSPVIRQAVKDHGLRSEASTRFEKGIDPARTKEALERAAALMSEYAGGEVVGGIVEASVWRQDSVVVTVTLERINGVLGTAMSKEEVAAILSNLQFPFTEDNGTFTIHVPSRRRDIAIEEDIIEEVARLYGYDRLPATLPVAEAKPGGLTPYQAKRRRVRRYLEGAGLFQAITYSLTSPDKATRFALETAEPIRLALPMSEERSVLRQSLIPHLLEAASYNRARQVENVALYEIGSVYLSKGEHVQPAENERLAGVLTGLWHAHLWQGEKKAADFYVAKGILDGLFDLFGLAARIEYKPAKRADLHPGRTAEIALGGKVIGFVGQLHPAVQKEYDLKETYVFELALTDLLNAESEAIRYEPIPRFPSVVRDIALVVDENVEAGALKQAIEEAGKPLVKDVSLFDVYKGDRLPDGKKSLAFS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with ATP binding, phenylalanine-tRNA ligase activity is needed to enhance phenylalanyl-tRNA aminoacylation in a specific context.2. The protein should have a high affinity for Mg(2+). The designed protein sequence is ",
    "response#1": "LKKVLDEVHDIFLGMGYNIEEGPEIETDYYNFEALNIPKGHPAREMQDSLYITEEILLRTHTSPVQIRTMEQVAPEIPVRIICTGKVYRKDDDATHSPMFHQIEGLVVGERITLGDLKGTLLNFAKQMFGPEVDIRLRPSYFPFTEPSAEVDISCVICEGSGCRVCKGTGWLEILGSGMVHPKVFEMSGYDPDKVTGFAFGMGVERIAMY",
    "response#2": "DASLFTALRPKSNGHPIYQTMDRIIDFFVNMNFAIQTGPLVEDDFHNFEALNLPVFHPARDMQDTFYFKDSMLLRTHTSPVQIRTMQSQHIPIRMIAPGSVFRRDYDLTHSPMFHQVEGLVVDEKDKISFVHLKYILEDFLHYMFGDVRIRFRSSFFPFTEPSAEVDISCVFCGGSGCRVCSHTGWLEVLGCGIVNQKVFDAVGHKNVSGYAFGLGVERFAMLLHRVNDLRSFFETDLRVLEQV",
    "response#3": "LSEQIADIFVGMGWEIADGPEVEAEYFNFDSLNFIPDHPARTLQDTFHIAPEGSGQVLRTHTSPVQMRTMLSRDLPIYIACPGRVFRTDELDATHTPVFHQVEGLAVDKGLTMAHLKGTLDHLAKTLFGEEAKTRIRPNYFPFTEPSAEVDVWFADKKGGAGWIEWGGCGMVNPNVLIAAGVDPEEYSGFAFGMGIERTLQFRNGLPDMRDMVEGDVRFTLR"
  },
  {
    "reference": "MLAALWLLLGAAFLVAVLVQLLQGPPGKPPLGLDCLFQDLIRYGKTKSGCGQRPALLQLLDVPKRWFSHFYIVSVIWNGLLLLLLIQALFLSRPLPVWLQDLLVVLGGAAQNQNAGGEHLSALLVLVLLWLHSLRRLAECLYISVFSDGVIHLVQYCFGLGYYVVMGLTVLCQMPARVGNGHFMKICWYHVLGIIMYVWASAHQHRCHVILANLRKSKSGKVVNLDHSIPVGDWFERVSCPHYFAELLIYISMAVTFGLHNLTSWLVLLYVLFNQALAACLCHEFYLTKFSSYPQHRKAFIPFIF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein should be able to manipulate the activity of enzymes in the Protein modification, protein glycosylation pathway, either by activating or inhibiting their function.2. The protein must exhibit the following characteristics: Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol.3. We need a protein that can perform di-trans,poly-cis-dolichol + NADP(+) = di-trans,cis-polyprenol   + H(+) + NADPH with high efficiency.4. The designed protein must be optimized for 3-oxo-5-alpha-steroid 4-dehydrogenase activity, polyprenol reductase activity.5. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "AGLVLFVGASLLQHQSMVLLARLRTGNTGRVETLAHRMPEGGCFQLVSCPHYFAELLIYVSLSLVFGGLSLTWWLVVLYVLFNQALAGQLSHELYISRYKSYPKCRRAFIPYVG",
    "response#2": "WIGGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYLGLLISSGGTDISIWLLFGFVAANLTYAAGETHRWYLQKFENYPASRHAIFPHVL",
    "response#3": "DGVIHTVQYCFGLGYYVALGLTVLCQVPANFRNGKVINLSHSVPYGDWFERVSCPHYFAEVLIYVSMAITLGLHNVTWWFVVLYVLSNQALAAVLCHEFYQNKFSSYPKHRKAFIPFVV"
  },
  {
    "reference": "MLFLTKIIDSNIKLQKSFDSKVKVSGIATDSREIRKGMIFAVIRGNKNNGKQYIKDAYKSGAKALLCEREHLKDIKTEVKNILVSKNIRLSVAVIAKKFYPKQPANISVVTGTNGKTSIVNFLYEIWKKNHIRGACFGTLGIKYDGKNKKTKLTTLDAIRLHKELNFIKSKKINFAAMEASSHALDQFRLDKVYVKFALFTNLSRDHLDYHQNMKKYFLSKIRLFKFILDKKGTAIINTDNRYGKKIKNICDIENIKNITYGFKKDSDWRITKVKREGKLTKVVFSKENIVYNFFCNLIADYEVENLIGAIIIANLNGMSLEKILKNIKSIKKPSGRLKKISKKNVRLSIFIDYAHTPEALEKSLKALKSVYDFKGNLILVFGCGGERDKGKRKFMGIVADNFADKVFITDDNPRYENAKNIRKEIASACKKAVCIPNRETAISRAINIMQENDILLIAGKGHEKYQEIKGSKIPFDDEVVVKEAINRKLA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of Mg(2+) is necessary for the protein to function in the desired environment.2. The protein contains novel Mur ligase C-terminal, Mur ligase central domains that confer a unique function or activity.3. A protein that contributes to cell cycle, regulation of cell shape, peptidoglycan biosynthetic process by functioning in cytoplasm with ATP binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity.4. Incorporating a Meso-diaminopimelate recognition motif motif is critical for the successful design of the protein.5. The designed protein should have a binding site that is specific to the target UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate ligand. The designed protein sequence is ",
    "response#1": "MKIVTGTNGKTTISQLVAQLAQLMGMSSAVMGTAGNGKLGALVQASHTTGDALAVQQFLYQMGQENADLLALEASSHGLDQQRLQGMPASVAIYTNLSRDHLDYHADMAEYAAAKAKLFDKTHFPELTHAIINLDDEHAQIMLDMAHTSALTVWTYSLNPTKNATFVAATINPSLQGVEIAVRYNVSNNTGDVHLDSTDANHDQTNTITVISPLLGRFNVANLLAAIAILLAQGVTLVTGTNGKTTISQLVAQLAQLMGMSSAVMGTAGNGKLGALVQASHTTGDALAVQQFLYQMGQENADLLALEASSHGLDQQRLQGMPASVAIYTNLSRDHLDYHADMAEYAAAKAKLFDKTHFPELTHAIINLDDEHAQIMLDMAHTSALTVWTYSLNPTKNATFVAATINPSLQGVEIAVRYNVSNNTGDVHLDSTDANHDQTNTITVISPLLGRFNVANLLAAIATLLALGYPLADVVRVLPQLQAPAGRMQLVTGHPSGARVIVDYAHTPDALRAALSALRPEAKGRLAVLFGAGGDRDPGKRSMMGKVAQNGADLVYVTDDNPRSEDPGDIRKAILDGCPDAVEIADRGAAITAALGELSAEDVLLIAGKGHEDYQIIGTTKHHFSDLEEAEKALQQWDAP",
    "response#2": "MKLSNLLHGLTHLQVHGDPQLQVDSRAVGEGDGFIAWPGAATDGRRFVQSALAQGAAACLVEADGSEAYIVPDTRVAMARASAAWFGGRPARTLRMIGVTGTNGKTTVTNMIAHILEEGQTRCGLIGTVYRHIGDRILDARNTTPDALELQRLLAEMADAGCEYCVMEVSSHALVQNRVAGCHFDVAVFTNLTQDHLDFHKTMEAYFEAKRKLFTEYLRPGAAAVVNIDDPYGERIIRETRARVLTFGIENPADLRAVDVRYGLDGVAAELVTPAGSFTVRSPLIGRHNVYNILGSVGVGLALGIPLADIVAGIARFRGVPGRMERIGEANGGLCLVDYAHKPEALEHVLMALRPFATGRLVLVFGCGGDRDTGKRPIMGEIAQRLADRVIVTDDNPRTEEPAAIRAAILAQAPGAEEIGDRAEAIRTAVRELQPGDVLVVAGKGHETYQLIGDTTLHFSDREVVREALEARK",
    "response#3": "MKLSRLLGEAGVSLRGDAALQVDSRAVGEGDGFIAWPGAATDGRRFVQSALAQGAAACLVEADGSEAYPYLVIPDTRAAAAPLAAAYFGHPARRLTLIGVTGTNGKTTVAFMDKSILEAAGRRTGVIGTLGARIGDRVLEAQHTTPESLDLQALLAQMRDAGCQAVAMEVSSHALVQHRVAGLEFAAGVFTNLTRDHLDFHGTMEAYLAAKALLFRGLDAQVAVVNADDPYGRRLAAQGGAARVLRYSLAGEAADCHPVSLAYTAEGIRGRLGTGDADFPLSFPLPGRFNLYNALGAIAACRALGVSLEEAVRALEGAAPPAGRFECIRSPRGVTAIVDYAHTPDALVNVLQTINEVLRHKGECWTVCGAGGNRDRGKRPIMAQEAVRNSDHVIITSDNPRYEDPQAIIDDMLAGLDAEQRQGVLSIVDRREAIRTACMLAKPGDVVLVAGKGHEDYQILGDRRIRFSDRETVREALL"
  },
  {
    "reference": "MAVAVRTLQEQLEKAKESLKNVDENIRKLTGRDPNDVRPIQARLLALSGPGGGRGRGSLLLRRGFSDSGGGPPAKQRDLEGAVSRLGGERRTRRESRQESDPEDDDVKKPALQSSVVATSKERTRRDLIQDQNMDEKGKQRNRRIFGLLMGTLQKFKQESTVATERQKRRQEIEQKLEVQAEEERKQVENERRELFEERRAKQTELRLLEQKVELAQLQEEWNEHNAKIIKYIRTKTKPHLFYILGRMCPATQKLIEESQRKMNALFEGRRIEFAEQINKMEARPRRQSMKEKEHQVVRNEEQKAEQEEGKVAQREEELEETGNQHNDVEIEEAGEEEEKEIGIVHSDAEKEQEEEEQKQEMEVKIEEETEVRESEKQQDSQPEEVMDVLEMLLHVAVKNVIAEQEVMETNQVESVEPSENETSKELEPEMEFEVEPDKECKSLSPVRENASALEMENEPEEKEERESEPQPEPVRHLQPLPQPEPEPELQPEPQPQLQPEPQLQPQLQLQLQPQPQSQSQPQPQLQLPLPLPLQPQPQVQAQSQPQAVLQPQPVSQPETLPLAVLQAPVQVIQEQGHLLPERKEFPVESVKLTEVTVEPVLIVHSDSKTKTKTRSRSRGRARNKTSKSRSRSSSSSSSSSSSTSSSSGGSSSSGSSSSRSSSSSSSSTSGSSRRDSSSSTTSSSESRSRSRGRGHNRDRKHRRSVDRKRRDTSGLERSHKSSKGGSSRDTKGSKDKNSRSDRKRSISESSRSGKRSSRSERDRKSDR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should emphasize Basic and acidic residues, Basic residues compositional bias.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in nuclear speck, desmosome that is critical for RNA splicing.3. The protein must exhibit the following characteristics: Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'; Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; Capable of reversing CTBP1-mediated transcription repression; Participates in the regulation of alternative pre-mRNA splicing. The designed protein sequence is ",
    "response#1": "GGKGGFGSQLRAAGGRMSKKRNEQENQDSCRDLDGNRLGTIRQAKELSEYLAKKPAETRAKKEAKKQKLNKVLAADSSSSRFDDHEYLEDLEQSVSNVRDAFSN",
    "response#2": "MAVAVRTLQEQLEKAKESLKNVDENIRKLTGRDPNDVRPIQARLLALSGPGGGRGRGSLLLRRGFSDSGGGPPAKQRDLEGAVSRLGGERRTRRESRQESDPEDDDVKKPALQSSVVATSKERTRRDLIQDA",
    "response#3": "MAVAVRTLQEQLEKAKESLKNVDENIRKLTGRDPNDVRPIQARLLALSGPGGGRGRGSLLLRRGFSDSGGGPPAKQRDLEGAVSRLGGERRTRRESRQESDPEDDDVKKPALQSSVVATSKERTRRDLIQDY"
  },
  {
    "reference": "MNKFNIAVVGATGNVGREILNXLCERNFPXKKIFALAXKXSEGKRVXYGSKKIKVQXLXTFNFKNADIVLSSAGSXVSESXVXKAXXAGAITIDNTSCFRMDKDVPLIVPEVNXKELSKSKKKKIIANPNCSTIQLVTALKPIHDIFKIKRXIVSTYQSTSGAGKKAMDELFTQTLDVXKNKSLKSEVFSKQIAFNIIPQIXXFLDDGKTKEELKLVQETKKIXDTKIKVFATCVRVPVFIGHALSVNIETQLTINEKKVRKVLKDFEGVNVVDDPSKSEYITPDEIAGKDEVFVSRIRKDDSVKNGLSLWIVADNLRKGAALXTVQIAXELIKKKVK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should take into account the physiological conditions of the Amino-acid biosynthesis, L-threonine biosynthesis pathway.2. The designed protein is the optimization of the Acyl-thioester intermediate to achieve high catalytic efficiency for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.3. The protein design must be able to perform protein dimerization activity in order to contribute to isoleucine biosynthetic process, diaminopimelate biosynthetic process, 'de novo' L-methionine biosynthetic process.4. The designed protein should have a high specificity for phosphate binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MKNLKPLHDAVGISRINVATYQAVSGTGKKAVTELINQVGELLNGRPPKSSVYPQQIAFNVLPHIDEFQDNGYTREEMKMVWETKKIMEDDSILVNPTTVRVPVLYGHSEAIHLELKAPLSVEKARELLKKAPGVVLVDDPAKLKYPTPITHAIGHDEVFVGRIRQDITHPNGLNMWVVADNIRIPPAGHLKPLHDFAKIKRIVVSTYQSVSGAGKDGVDELWNQTKGIFVNDSATPGKFPKQIAFNVIPHIDVFMEDGYTKEEWKVLAETKKILDPKIKVTCTAVRVPVFVGHSEAVNIEFENPISADEARDILREAPGISVLDKREPGGYATPVEVVGEYDTYVSRIREDNTIENGLNIWVVSDNLRVPPAGHTLPQDLKPIHDQFKIKRVVVSTYQSVSGSGQKAIEELHSQAQSVLSGKPPENNVYPHQIAFNCLPHIDNFLDSGYTKEEMKMVNETRKILEDDSIQISPTTVRVPVFYSHSESVNVETEKPISAGEVKKLLSDAVGVMVVDNPETNEYPLAIDGEGRDDVFVGRIRDDISCENAINFWVVSDNLRVPPAGHTLPQDLKPLHDRYRVRRVVVSTYQSVSGSGQKAIEELKQQSRKILDGEPVQCNVYPHQIAFNCLPHIDIFMENGYTKEEMKMVDETRKILGDNTIEVSPTAVRVPVFYSHSESVCVETELPMGVDEIKDLLSSSPGISLVDNPEKNEYPLAVDACGKGGVFVGRVRGDLVRDNVINFWVVSDNLL",
    "response#2": "LKPLHDRYRVRRVVVSTYQSVSGSGQKAIEELKQQSRKILDGEPVQCNVYPHQIAFNCLPHIDIFMENGYTKEEMKMVDETRKILGDNTIEVSPTAVRVPVFYSHSESVCVETELPMGVDEIKDLLSSSPGISLVDNPEKNEYPLAVDACGKGGVFVGRVRGDLVRDNVINFWVVSDNLLSWK",
    "response#3": "RVAVVGATGAVGREMLTTLAERNFPADEIIALASGRSAGNEVSFGEKGTLTVQDLATFDFSKADIALFSAGGDVSLEYAPKAGQAGCVVIDNSSAFRMDPDVPLVVPEVNPGALAGFTKKGIIANPNCSTIQMLVALAPIHRAVGIDLINISTYEAVSGSGRKGVKELAQQTAQLLNAREIESRVYDKQIAFNVIPYVGSFLENGYTHEEMGLVWETQKVLDDTSVRVNPTCVRVPVFFGHSASVYIETLAYISAEEVIDILETTEGVEVLSSVADMSFATPVSDAAGSDAIYVSRIREDLNGRQGISLWITADNVRKGAALNSVQIAELLI"
  },
  {
    "reference": "MVADPPRDSKGLAAAEPTANGGLALASIEDQGAAAGGYCGSRDQVRRCLRANLLVLLTVVAVVAGVALGLGVSGAGGALALGPERLSAFVFPGELLLRLLRMIILPLVVCSLIGGAASLDPGALGRLGAWALLFFLVTTLLASALGVGLALALQPGAASAAINASVGAAGSAENAPSKEVLDSFLDLARNIFPSNLVSAAFRSYSTTYEERNITGTRVKVPVGQEVEGMNILGLVVFAIVFGVALRKLGPEGELLIRFFNSFNEATMVLVSWIMWYAPVGIMFLVAGKIVEMEDVGLLFARLGKYILCCLLGHAIHGLLVLPLIYFLFTRKNPYRFLWGIVTPLATAFGTSSSSATLPLMMKCVEENNGVAKHISRFILPIGATVNMDGAALFQCVAAVFIAQLSQQSLDFVKIITILVTATASSVGAAGIPAGGVLTLAIILEAVNLPVDHISLILAVDWLVDRSCTVLNVEGDALGAGLLQNYVDRTESRSTEPELIQVKSELPLDPLPVPTEEGNPLLKHYRGPAGDATVASEKESVM",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: (Microbial infection) Acts as a cell surface receptor for type D simian retroviruses..2. The designed protein should have at least Helical; Name=2, Helical; Name=8, Helical; Name=7, Helical; Name=6, Helical; Name=3 transmembrane domains to ensure proper localization.3. The designed protein should be involved in glutamine transport, L-aspartate import across plasma membrane, neutral amino acid transport, protein homotrimerization, L-glutamine import across plasma membrane by localizing to basal plasma membrane, membrane, extracellular exosome, plasma membrane and exhibiting L-serine transmembrane transporter activity, neutral L-amino acid transmembrane transporter activity, L-glutamine transmembrane transporter activity, symporter activity.4. The designed protein should contain Cytoplasmic, Extracellular topological domains.5. The Na(+) binding site should be located in a region of the protein that is accessible to the ligand.6. helix-helix-helix-turn-helix-helix-helix-strand-helix-helix-turn-helix-helix-strand-strand is the desired arrangement for the engineered protein. The designed protein sequence is ",
    "response#1": "VMLVQAIGGSLNGYSIGFVGVYSTLFGYSTNCASFLQENSCTTVPNADCKWFVSPTGSSYCGWPEVTCRKEYAYSSPAEMPGALARCEADSRCRWSYSDEECQNPSGYSSSESGIFAGSMIAGCLIGSVFAGPLASKIGARLSFLLVGLVGVVASVMYHASCAADEFWVLIVGRFVIGLFLGVICVACPVYTDQNAHPKWKRTIGVMFQVFTTLGIFVAALMGLALGQSIRFDHDGDQKVMARMQGLCVFSTLFSLLTVVLGIVTRESRAKFDGGEEGRAELNPSEYGYVEMIPRLLMGCVMAGTLQLTGINAVMNYAPTIMGSLGLAPLVGNFVVMLWNFVTTLASIPLSYVFTMRHVFLFGSIFTSCMCLFMCGIPVYPGVSKKLEAKNGVAITGILLFILGFEVCVGPCYYVLTQDMFPPSFRPRGASFTQVAQFIFNLIINVCYPIATESISGGPSGNQDKGQAVAFIFFGGLGLICFVIQVFFLHPWLFSLYHVSS",
    "response#2": "VMLVQAIGGSLNGYSIGFVGVYSTLFGYSTNCASFLQENSCTTVPNADCKWFVSPTGSSYCGWPEVTCRKEYAYSSPAEMPGALARCEADSRCRWSYSDEECQNPSGYSSSESGIFAGSMIAGCLIGSVFAGPLASKIGARLSFLLVGLVGVVASVMYHASCAADEFWVLIVGRFVIGLFLGVICVACPVYTDQNAHPKWKRTIGVMFQVFTTLGIFVAALMGLALGQSIRFDHDGDQKVMARMQGLCVFSTLFSLLTVVLGIVTRESRAKFDGGEEGRAELNPSEYGYVEMIPRLLMGCVMAGTLQLTGINAVMNYAPTIMGSLGLAPLVGNFVVMLWNFVTTLASIPLSYVFTMRHVFLFGSIFTSCMCLFMCGIPVYPGVSKKLEAKNGVAITGILLFILGFEVCVGPCYYVLTQDMFPPSFRPRGASFTQVAQFIFNLIINVCYPIATESISGGPSGNQDKGQAVAFIFFGGLGLICFVIQVFFLHPWRSLRYFREKKAIVQKLSAIESLAGVEILCSDKTGTLTKNKLSLHEPYTVEGVSSDDLMLTACLAASRKKKGLDAIDKAFLKSLAQYPKAKGALTKYKVLEFHPFDPVSKKVTAVVESPEGERIICVKGAPLFVLKTVEEDHPIPEDVHENYENKVAELASRGFRALGVARKRGEGHWEILGVMPCMDPPRDDTAATVNEAKRLGLSVKMLTGDAVGIAKETCRQLGLGTNIYDAERLGLGGGGSMPGSEMYDFVENADGFAEVFPQHKFAVVDILQQRGYLVAMTGDGVNDAPSLKKADTGIAVEGATDAARSAADIVFLAPGLSAIIDALKTSRQ",
    "response#3": "VMLVQAIGGSLNGYSIGFVGVYSTLFGYSTNCASFLQENSCTTVPNADCKWFVSPTGSSYCGWPEVTCRKEYAYSSPAEMPGALARCEADSRCRWSYSDEECQNPSGYSSSESGIFAGSMIAGCLIGSVFAGPLASKIGARLSFLLVGLVGVVASVMYHASCAADEFWVLIVGRFVIGLFLGVICVACPVYTDQNAHPKWKRTIGVMFQVFTTLGIFVAALMGLALGQSIRFDHDGDQKVMARMQGLCVFSTLFSLLTVVLGIVTRESRAKFDGGEEGRAELNPSEYGYVEMIPRLLMGCVMAGTLQLTGINAVMNYAPTIMGSLGLAPLVGNFVVMLWNFVTTLASIPLSYVFTMRHVFLFGSIFTSCMCLFMCGIPVYPGVSKKLEAKNGVAITGILLFILGFEVCVGPCYYVLTQDMFPPSFRPRGASFTQVAQFIFNLIINVCYPIATESISGGPSGNQDKGQAVAFIFFGGLGLICFVIQVFFLHPWLFIGTLVAVITGAGLPLMSILQGKVSQAFINEQIVINNNGSTFLPTGQNYTKTDFEHDVMNVVWSYAAMTVGMWAAGQITVTCYLYVAEQMNNRLRREFVKSILRQEISWFDTNHSGTLATKLFDNLERVKEGTGDKIGMAFQYLSQFITGFIVAFTHSWQLTLVMLAVTPIQALCGFAIAKSMSTFAIRETLRYAKAGKVVEETISSIRTVVSLNGLRYELERYSTAVEEAKKAGVLKGLFLGISFGAMQASNFISFALAFYIGVGWVHDGSLNFGDMLTTFSSVMMGSMALGLAGPQLAVLG"
  },
  {
    "reference": "MRAAHHPDDVRRIEQAQGELFRDGTLMQRAARAVAKAVDHQLGDGGRVLALVGPGDNGGDALWSLAMLAGESGHPCTALLVAEHAHEDGLAAARQAGAQVLDPGEWEHPPRRPGGVSWGPGDVVVDGIVGLSGRPGLTGLAAELAVWLDGLREGATAPHVVAVDLPSGADPDGRQPTHPGEFIRADTTVAPITAKPVHLLPGTRRACGEVVIAPLGADVSGEPPAVREMDHHDLAGSYPWPTPADHKYSRGVLGVVAGSDAYPGAAVLTCSAALHTGVGMVRYAGPQRATDQVLARCPEVVPGQGRVQAWLLGPGTSGDDRARREEMRELARRPEPCVLDAGALALVVERPAGSATVLTPHAGELAGLLGWERTDVESDPVGAGLEAADRWGVTVVVKGACTVVVPASGDRNQGVSVLDGGTAWMATAGTGDLLAGAIGALLALGVTPHDAASLGVALHQRAGVLASGGGPLRGLQLARHLSTAVAELLPAPERGSSRAS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be optimized for NADHX epimerase activity, NADPHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity activity in order to improve phosphorylation.2. The protein must exhibit the following characteristics: Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. The designed protein sequence is ",
    "response#1": "MDQHKGQGGHTVVMGGSIPYLGAATMAGLGAVASGAALVTLSVHSSLAKEARMGYPGLMVASGNPEAPGWPRGKVASLVIGPGWGDEGLAYWFAVVMGDGPWCGLPTVFDADALNILAHDPGLRACDLPLNPSILTPHTGEAARLLAMSAEQVLENRVAAARVLAEKHRAVVVLKGPSTLIAAPDGRLAVNPTGHPLLAQGGTGDVLAGIIGGLQAQGMASFEAACCGTFLHGWAAERLAEHQGESGLRPELLR",
    "response#2": "PDSHKGTFGHLLVVAGSEGMTGAAALAGEAAMRVGSGLVSVAVPQSLRTVIEMKLTECLKLGLEESEPGVLGEGAVRQLTPHLHRYHATLLGPGLSTRGEVARVVVALIERAEHPMVLDADALNILAGTLESLEVAKAPLILTPHPGEMARLLRTSTARVQENRLSIAHDFATRHGVVLVLKGARTLIATPEGEVFVNPTGNAGMASGGTGDVLAGMIGGFLVQGHPPRTAALLGVYLHGLAGDLAAEALGERSFIASDLLDRLAA",
    "response#3": "MDQHKGQGGHTVVMGGSIPYLGAATMAGLGAVASGAALVTLSVHSSLAKEARMGYPGLMVASGNPEAPGWPRGKVASLVIGPGWGDEGLAYWFAVVMGDGPWCGLPTVFDADALNILAHDPGLRACDLPLNPSILTPHTGEAARLLAMSAEQVLENRVAAARVLAEKHRAVVVLKGPSTLIAAPDGRLAVNPTGHPLLAQGGTGDVLAGIIGGLQAQGMASFEAACCGTFLHGWAAERLAEHQGESGLRPELLA"
  },
  {
    "reference": "MGSAAGETASRKPRVVLGVSAGIAAYKACELLRLFTESGHDVRVVPTREALKFVGAPTWAALSGHPVASEVWEDVHEVPHVRIGQEADLVVVAPATADVLAKAACGLAGDLLTNTLLTARCPVVFAPAMHTEMWEHPATRANVATLRERGSLVIDPAVGRLTGADTGPGRLPEPREIFEVCRAILSGRPRDLAGRRVVVSAGGTREALDPVRFLGNRSSGLQGYALARAAVARGAQVTVVSANVTLDDPAGATVVRVESARDMRDAVLAAYGTADVIVMAAAVADFRPAERSGSKIKKTSDEPDPIHLTKNPDILAELGALRRDTGGPGPAVIVGFAAETDDILANGQAKLKRKGCDLLVVNEVGENLAFGTPDNAAVILSADGPAVEVPRGPKDALAHVVLDLVAPRLA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should interact with enzymes and substrates within the Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis pathway to enhance or regulate its activity.2. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domain.3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.4. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.5. The protein have enhanced H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.6. The designed protein should be able to compete with endogenous proteins or inhibitors for CTP binding.7. The designed protein should be optimized for metal ion binding, phosphopantothenate--cysteine ligase activity, FMN binding, phosphopantothenoylcysteine decarboxylase activity activity in order to improve pantothenate catabolic process, coenzyme A biosynthetic process. The designed protein sequence is ",
    "response#1": "MNIVLGITAGIAAYKTPQLVRLLTKNGHTVKVILTENAKEFVTPLTLSTVSKNTVFSAFTSVENDWHSHVELALWADVMLIAPATANTIAKMAHGICDNLLLATYFSAKAPVFVAPAMDLDMYAHPTVTENLKQLQSFGNHIIPAAYGELASGLIGQGRMAEPEDIVTFLEKALGISHKWLAGARVLVTAGPTREAIDPVRFLSNRSSGKMGYALAAAASRRGAKVTLVSGPTALAAAPGVELVPATSAAEMARAVRSRFSRCDALLMAAAVADFTVQRPARSKIKKDRIRGPLRLALVPTEDILASLGRSRRGRRPWLIGFAAETGDPVPAAKDKLARKRLDMVVANDVSEKGAGFEVDSNHLYLFWKNGSKHLPLTPKQQLAHVLLNEIARQS",
    "response#2": "MNIVLGITAGIAAYKTPQLVRLLTKNGHTVKVILTENAKEFVTPLTLSTVSKNTVFSAFTSVENDWHSHVELALWADVMLIAPATANTIAKMAHGICDNLLLATYFSAKAPVFVAPAMDLDMYAHPTVTENLKQLQSFGNHIIPAAYGELASGLIGQGRMAEPEDIVTFLLNHYFQPRNLEGKRIVITAGPTREMVDPVRFFSNRSTGKMGYALAEAALQLGGEVTLISGPTDLSPPRGAHFIQVNSAEEMYEAVHSVFNQADIVVKSAAVADYRPKEILDQKLKKKEGSLVLEFERTRDILKSLGERKDGQFLIGFAAETTNVEEYAQAKLHKKNADMIIANNVKEAGSGFGTDTNKVSILFANGESIDLPLMSKKEIAHEILLQILRLMKGRQ",
    "response#3": "MNIVLGITAGIAAYKTPQLVRLLTKNGHTVKVILTENAKEFVTPLTLSTVSKNTVFSAFTSVENDWHSHVELALWADVMLIAPATANTIAKMAHGICDNLLLATYFSAKAPVFVAPAMDLDMYAHPTVTENLKQLQSFGNHIIPAAYGELASGLIGQGRMAEPEDIVTFLESFFA"
  },
  {
    "reference": "MMRPQPPLFRPSIPMPHGLGDREAAILREIVEEYVETGEPIGSQTLAQRLQPSLSPATIRNVMAELARAGLLFSPHVSAGRLPTEKGVRLFVDGLLQFSSLTEEDRASIDSRLDIHGRSYQDILSEASSLLSGLSSAAGLVLAPKSDATLKHIEFVALGPGRVLVILVGSDGQVENRIIETPPGVPPSALVEAGNYLNSRLGDLTLGNLRERVRAEMDASRHELDALTASVIESGLATWDADGGTLFVKGQGKLLADITEIERLTTIRMLFDHLETQETMLQLLQLADASEGVRIYIGRESGMFGMSGVSMIVAPARNEAQKIVGAIGVIGPTRLNYGRIVPVVDYTARMVGRLLG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Prevents heat-shock induction of these operons. The designed protein sequence is ",
    "response#1": "LVNATTTLSGLSACAGLVLVPKQELVLKQLGFVVLDDNRALAIIVGSDGSVENRVIELSSGFPASALTEASNYINAHFSGYSFSEAKKRLFSQIDLERSELDSAASDLIKRGLAVWSEDSRKRPVLIVRGQSHLLQDASEDLDRAKQLLEELEDKKEIAGLLEKVSESDAAQIFIGSENKLFSLSGSSVIASPYHGEDGHMVGVVAVIGPTRLNYGRIR",
    "response#2": "LVNATTTLSGLSACAGLVLVPKQELVLKQLGFVVLDDNRALAIIVGSDGSVENRVIELSSGFPASALTEASNYINAHFSGYSFSEAKKRLFSQIDLERSELDSAASDLIKRGLAVWSEDSRKRPVLIVRGQSHLLQDASEDLDRAKQLLEELEDKKEIAGLLEKVSESDAAQIFIGSENKLFSLSGSSVIASPYHGEDGHMVGVVAVIGPTRLNYGRIR",
    "response#3": "LVNATTTLSGLSACAGLVLVPKQELVLKQLGFVVLDDNRALAIIVGSDGSVENRVIELSSGFPASALTEASNYINAHFSGYSFSEAKKRLFSQIDLERSELDSAASDLIKRGLAVWSEDSRKRPVLIVRGQSHLLQDASEDLDRAKQLLEELEDKKEIAGLLEKVSESDAAQIFIGSENKLFSLSGSSVIASPYHGEDGHMVGVVAVIGPTRLNYGRIR"
  },
  {
    "reference": "MGDLYGKTIVLGVCGGIAAYKAAALTSKLTQAGATVRVIMTKSAVQFVAPLTFQTLSRHHVFVDTFDEKDASVVSHINLADSADLVVIAPATANTIGKLALGLGDDMLSTTLLATMAPVWVAPAMNVHMYANPAVQQNMQTLRSRGVRFIEPGEGQLACGYVGKGRLAEPEEIFATIEQYFGGGSRLKGRHVLVTAGGTVERIDPVRYITNDSSGKMGYAIAEEAVRMGATHVTLVTGPTALSAPAGVELIPVKSALDMRDAVLSRLAANHLIIKAAAVADYRPAAVAEQKMKKKTESLTLELVKNPDILQEIGTLKTEQFVIGFAAETERLDEHALDKLKRKNCDLIVGNDVSQDGAGFSGDTNVVRIFDHNGLVEALPIQSKHLVARRLLELAAERMPRLAHTTGIPS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.2. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..3. The designed protein must be optimized for phosphopantothenate--cysteine ligase activity.4. The protein design should take into account the physiological conditions of the CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis pathway.5. The presence of Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "KTVLLAVTGSIAAYKIPNLVRMLTKLNCNVRVLMTQNATQFINPITFETLTGSKCLIDTFDRNFQYSVEHVALAKQADVVMIAPASANVIGKIACGIADDMLTTTVMACPCKKIVSPAMNHNMYHNPIVQDNIKKLAAYGYEIALPDTGRLANGDIGDGRMPEEDLIDEVKWVLLKGKHILVTSGPTHEPIDPVRYIANRSSGAQGTAIAIALRDLGADVSFVTGPADVPPPLGLRVIPVETAREMKAAVEAALPAEAAVFAAAVADWRVASESGSKIKKTQGKLPILEFSENPDILAGVSQMAEGRPRLVVGFAAETDDVIAHATEKRKRKGCDWIVANDVSPATGIMGGTENAVTLITHNGVEDWPQMDKDLLAKQLVERIADYFQ",
    "response#2": "VALGVTGGIGAYKAVEIARGLQKRGHDVVAIMSHGALKFVGEVTFEAITKRRVITDQYAPGANADIEHISLATDIGLLLVAPATANVIGKFAHGIADDFLTSLYLATRAPVLMAPAMNTNMLEHDAVSRNLATLTARGVRFVDPGQGYLACGWVGKGRLAEPDDVVEAIAAGLRPASLAGRHVLVTSGPTHEPIDPVRYIANRSSGAQGTALAAALRDLGAKVTFVTGPATVPPPQGVTVVKVETAQQMLAAVEAALPAEAAVFAAAVADWRVANAADRKMKKDGSGTPPALSFAENPDILATISQLAKGRPRLVVGFAAETDDVIAHATAKRARKGCDWIVANDVSPATGIMGGSENAVTVHLVGDDGVEDWPRMSREAVARRLAERIAEALG",
    "response#3": "KSILLIIGGGIAAFKSLDLIRRLREEGVRVVPVLTKAAEEFVTPLSVSALAAEKVYRDLFDLTDEAEMGHIELSRAADLVVVSPATADLLGKMAGGLANDLASTLLMATDKPVLVAPAMNVRMWEHPACQRNVATLMGDGVLFVGPEEGSMACGEFGPGRLSETPDIVAAIRELLSPQEGPLGLTVLVTAGPTRELLDPVRFFSNHSSGRMGYALARAARERGARVLLISGPTALEPPPGVEFHPVVSAREMYDVVMDKLFLADVIIKTAAVADYRPEETYSQKIKKGGDLLVRLVRNPDILQELGSRKGNYILVGFAAETENLLNNAHKKLAAKNLDLIVANDLTEEGAGFTYETNKVNLLFADGTVEELPLLDKTEVADRILDRVVRLLGS"
  },
  {
    "reference": "MFVRKPATERPRLPSVGRLGLFDPHAPAHLDQLGWNTDDHVELLWSLSRAPDADAALLAMVRLADELKDDWDELNRLLLTDRPLRGRLFAVLGSSLALGDHLVAHPQSWRLLHGDVRLPSARELRETFDDAARSVDIERGASAAIPPLRDLYRDRLLVLAALDVASTVENEPVLPFVTVSAHLSDLADAALSAALIVATRTVCGDLDPRLAVIAMGKCGARELNYVSDVDVIFVGEDIEKDGRSDNLATATRVAGEMMRFAGDAFFEVDAALRPEGKRGQLVRTLDSHVAYYRRWAKTWEFQALLKARPAAGDPELGQAYIDALMPMVWTACEREDFVPEVQAMRRRVEELVPADVRSREIKLGTGGLRDVEFAVQLLQLVHGRTDESLHVASTVDALAALGEGGYVGRDDAANMTASYEFLRLLEHRLQLQRLKRTHMLPDDNDDEAYRWLARAAHIRPDGTHDAQGVLREELKRQSLRVSRLHAKLFYQPLLESVGHTALGIGEGMSAEAAERQLAALGYERPQSALAHLAALTGATGRKGRIQQILLPTLLDWLSDTPDPDAGLLAYRRLSDEHSDLRWFLGTLRDEGAVAKRLMRVLGTSAYIPELLMRAPEVIQMYADGPSGPKLLEDDRESRARALVASASRYADPVRAIAAARTLRRRELARIASADVLGMLDVIDVCKSLTAVWVAVLQAALDVVIRANTPDSGVPARIAVIGMGRLGGGELGYGSDADVMFVCEPNSGVEESVAVRWSVGVAEQVRALL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity.2. The designed protein must be able to regulate regulation of glutamine family amino acid metabolic process.3. We are looking for a protein with broad substrate specificity for [glutamine synthetase]-L-tyrosine + ATP = [glutamine   synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + diphosphate. The designed protein sequence is ",
    "response#1": "KRLVTVLGVSDAMGKFMRFKPQLVEAAAVDNCNSHLFNHAQRRARLLKAVGADPDEPAMPVASKDLAEAATALRSSYRNQLAAIIAQDAVADDPTSIQPTISRELSDLADAALEGALAIARHETEGSEHVRFTIIGMGKLGAQELNYVSDVDLIYVVEPADKDVDHQTLIRVGTKMGTMLQRVCQSAIMGVAEQPLWQIDGGLRPEGKDGALVRVLSSHKNYYEQWAENWEFQALLKARPVAGDPDLGQLERLCGHSIMVAEQIARYPMLLDEFIINKSLMKVIEFEQYKTELTEYLIRIPEEDEEALIDALRQFKQGHLLRIAVADILGVLPVMKISDHLTYLAAAIISEVVNMAWKSLVKRFGKPTHLAEDERGFAVIAYGKLGGLELGYNSDLDLVFLHNAPLEGETSGRRSISNHQFYLKLAQKINSIFNLNTSAGVLYEVDMRLRPVRRVADLVGASRWAAQYLTRHPILLDELLDDRNLEPAPDWDALRAQMRDTLDTLEPDMERQMDVMREQHHAQVFRLLTQDIAGLLTVEKLADHLSALADLMLELALPLVWRKIKIRHRDDPAFAVIAYGKLGGKELGYASDLDIVFLFDDPAPEALEVYTRLAQRINTWLSSQTAAGILFETDLRLRPNGESGLLVTSIESFRKYQLAQVALASDFALDTLARQPALLQHLAHPDPPPLPLPQLDPAQPQLWPAQLRRYRSAESTRLVWRDVLGLDSVEATLAGATRLAEHCMQCGLQALEQQFATRHGKVIADDGSVQRLVVFGLGKLGGGELNFSSDVDLVYAYPQGGQSDGARSLAAEEYFARLGQQLARLLDEATAEGFSHRVDLRLRPFGTAGRVALSFAGMDQYFQREGRDWERYAWLKARAVAGAIDAGEA",
    "response#2": "GQLRYAIAMSDFFAETLQKQPHFLHQCWQQCPRLQDCEQYAERLTPLLAQVENEEQLYKVLRQFRHREMAKLSFCQSLNLGSVEDIFIRLSQLAESVIIGARDWLYQRACAEMGTPVDEQGTPLQLYILGMGKLGGFELNFSSDIDLIFTYASNGETVGARRSIDNAKFFTRLGQRLINALDQYTCDGFVYRTDMRLRPFGENGALALSFAAMELYYQEQGRDWERYAMIKGRILGANAQDPHVNTLV",
    "response#3": "QFQFISGLSKFVVETVQRDAQLAEGLPEMLAQESRQEAYRTRLAEQLQACHDEVAGHRVLRQFRNREMVYIAWKDFTQAWSLEASLSHLSELAEAMIFETYQWQYQLCCQEWGTPTNAQGEAQPMLIIGMGKLGGGELNFSSDIDLIFTYPENGETQGARRSIANAQFFTRLGQRLIKALDQQTFDGFCYRVDMRLRPFGESGPLVMSYAALEDYYQEQGRDWERYAMIKARVMGREMYPEYQELFSAVMTLSPFLREVLIANPSYLSPLLYVDIETRLSEIIDDVALIDKSESINETALMAALRRKKREAHILIALADLSGVFTYEISCTWLTRLGEAALGVALRFLLREAHDHGKISLSSRDNPEKDSGLIILGLGKLGAGELNYSSDIDLIVFIDEMSPHIGDLSESVDVFSKMVRRLIRIIQERTAEGYVFRLDFRLRPDPGSTPLALPVRTALRYYEGRGQNWERAAMIKARPVAGDKRAGFKFLSAVMTLSPFLREVLIANPSYLSPLLYVDIETRLSEIIDDVALIDKSESINETALMAALRRKKREAHILIALADLSGVFTYEISCTWLTRLGEAALGVALRFLLREAHDHGKISLSSRDNPEKDSGLIILGLGKLGAGELNYSSDIDLIVFIDEMSPHIGDLSESVDVFSKMVRRLIRIIQERTAEGYVFRLDFRLRPDPGSTPLALPVRTALRYYEGRGQNWERAAMIKARPVAGDKRAGFKFLC"
  },
  {
    "reference": "MKVKGIRKNYQRLWRWGTMLLGMLMICNAADQLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVELENVTENFNMWKNNMVEQMQEDIISLWDQSLKPCVKLTPLCVTLNCTDAKGTNAKGTNVTDNGTSTPSPTMETGELKNCSFNITTDLGDKRQKEYALFYNLDVVQINRDNTSYRLISCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCRDKKFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENLTDNAKTIIVQLNESVIINCTRPNNNTRRSIHMGPGGAFYTTGQIIGDIRRAYCEINATKWNNTLKQIAIKLGEQIENSTTIIFKPHSGGDPEVVMHSFNCGGEFFYCNTTALFNSTWNITDLVHNNNTERLNTTNSTIILPCKIKQIINMWQGVGKAMYAPPIAGIINCVSNITGLLLTRDGGGNGNDTRRDNETFRPEGGNMKDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAIGGLGALFLGFLGMAGSTMGAASLTLTAQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLMGIWGCSGKLICTTAVPWNSSWSNKSQAEIWGNMTWMQWEKEINNYTDLIYTLITESQTQQEKNEKELLELDKWANLWNWFSITNWLWYIRIFIIIVGGLIGLRIVFSILSIVSRVRQGYSPLSFQTHLPAQRGPDRPEGTEEEGGERDRDTSGQLVNGLLAII",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should include a Di-leucine internalization motif motif to ensure proper function.2. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.3. The protein must exhibit the following characteristics: Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane.4. The protein design should emphasize Basic and acidic residues compositional bias.5. The protein should have a Helical domain that is long enough to span the entire membrane.6. The designed protein have Retroviral envelope protein GP41-like domains with specific sequence motifs that are crucial for its activity.7. The protein should have a specific function in viral protein processing, fusion of virus membrane with host endosome membrane and should be localized to host cell plasma membrane, host cell endosome membrane in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "MRVKGIQMNWQHLWRWGTMLLGMLMICSATEQLWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWVTHACVPTDPNPQEIVLGNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDANVTYNGLWVTVYYGVPVWKDADTTLFCASDAKAHETEVHNIWATHACVPTDPNPQEIPLENVTENFNMWKNKMAEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTKVTLKSTTNVTVNSTTNVSNVSDFANEVRNCSFNMTTEIRDRKQRVHALFYRLDLVPMNNDNNNSEYRLINCNTSVIKQACPKITFDPIPIHYCTPAGYAILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEKEIIIRSENLTNNAKTIIAHFNKSVVINCTRPFNNTRTSVTIGPGQVFYRTGDITGDIRKAYCEINGTNWFEALKQVAGKLKEHFPDKSIAFQPHSGGDLEITTHHFICRGEFFYCNTSQLFNITYTEGNETIEESNGNITLPCRIKQIINMWQGVGQAMYAPPVSGKINCVSNITGILLTRDGGAGTGDNNTETFRPAGGNMKDNWRSELYKYKVVQIEPLGLAPTRAKRRVVEREKRAVGIGALFLGFLGVAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKIICTTAVPWNSTWSNRSFEEIWNNMTWIEWEREISNYTSQIYEILTESQNQQDKNEKDLLELDKWTSLWNWFDITNWLWYIKIFIIIVGSLIGLRIIFAVLSIVNRVRQGYSPLSLQI",
    "response#2": "MRVKGIRRNYQHLWKWGTLLLGMLMICSAAENLWVTVYYGVPVWKEANTTLFCASDAKSYDREAHNVWATHACVPTDPNPQEVFLENVTEKFDMWKNYMVEQMHADIISLWDQSLKPCVKLTPLCVTLNCSDATSNGTNSNETMGNSEIKNCSFNATTEIRDKKQKGDALFYRLDVVPLNVTRNLRILEYILISCXTSTITQXCPKVSFXPIPIHYCAPAGYAILKCNNKTFNGTGPCQNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTDNVKTIIVHLNKSVPIVCTRPNNNTRKSIRIGPGQTFYATDIIGDIREAHCNIRAESWSETLKRVKKKLEEHFPNKTITFAQSSGGDLEVTTHSFNCRGEFFYCNTTKLFTIDLNTTTSSNDSITIPCKIKQIINMWQGVGRAMYAPPIAGNITCKSNITGLLLHRDGGVNGNNTTETFRPGGGNMKDNWRSELYKYKVVEIKPLGIAPTQAKRRVVEREKRAVGIGALFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQTFLGLWGCSGKVICTTPVPWNSSWSNKSYEDIWNNMTWTEWEREISNYTDIIYTILTESQNQQDKNEKDLLALDQWASLWNWFSISKWLWYIKIFIMIVGGLICLRIVFAVLSIVKRVRQGYSPLSFQTPHQQREPDRPERIEEE",
    "response#3": "MKVKGTRKNYQHLWRWGTMLLGMLMICSAAEQLWVTVYYGVPVWKDADTTLFCASDAKAHETEVHNIWATHACVPTDPNPQEIPLENVTENFNMWKNKMAEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTKVTLKSTTNVTVNSTTNVSNVSDFANEVRNCSFNMTTEIRDRKQRVHALFYRLDLVPMNNDNNNSEYRLINCNTSVIKQACPKITFDPIPIHYCTPAGYAILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEKEIIIRSENLTNNAKTIIAHFNKSVVINCTRPFNNTRTSVTIGPGQVFYRTGDITGDIRKAYCEINGTNWFEALKQVAGKLKEHFPDKSIAFQPHSGGDLEITTHHFICRGEFFYCNTSQLFNITYTEGNETIEESNGNITLPCRIKQIINMWQGVGQAMYAPPVSGKINCVSNITGILLTRDGGAGTGDNNTETFRPAGGNMKDNWRSELYKYKVVQIEPLGLAPTRAKRRVVEREKRAIGIGAVFLGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQNNLLKAIEAQQHLLQLTVWGIKQLQARVLAIERYLKDQQLLGLWGCSGKLICTTAVPWNASWSNKSLNMIWDNMTWMEWEREVSNYTDIIYSLIEESQNQQEMNEKELLELDKWASLWNWFNITNWLWYIKIFIMVVGGLVGLRIVFAVLSIINRVRQGYSPLSFQTLTPNPRGPDRLGGIEEEGGEQD"
  },
  {
    "reference": "MPPSHSVAQIRDAERQTLATGRRLMPLAGAAAARHVAARIRPGAVVLALAGPGNNGGDALEAATGLRAMGHDVRVLLPSGAQGLPADAARAYAGWQAAGGETWSTLEPGFVPGLVIDGLFGIGLNRPLGADWQALVDTVNAWNVPVLALDVPSGIDADSGEALGRPIQARWTLSFIGRARGLERPGPGRDACGISEVDTLGVIMTPAN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.2. The protein design should incorporate a binding pocket that specifically recognizes K(+) to enhance enzymatic efficiency.3. The protein must have high specificity towards nucleotide binding. The designed protein sequence is ",
    "response#1": "PDSYKNAHGHVLLIGGNRGMAGAIRLAAEAALRAGAGLVSVATHPDNITSVLQGRFELMVHGVESTNELSPLMKKASVIVLGPGLGQDAWAKALFNCAMVTELPMVVDADGLNCLAHAPRQKSNWVLTPHLGEARRLLSHSKTPLNEHDRFAVAKKISHEYGAVTVLKGPGSLISEGERININRSGCAGMASAGMGDVLSGIIGGLIAQGMEAFAATNLAVYIHGLAAEQAAHDGEKGMLAGDLFEHIINAPVPLPERVRWGHKGTFGKFLAVAGARYYYGAPYYVAYSFLKAGGGYSRLAAPRSVVPYIAARCSEVVYIPLEETEEGTISTRNLDYILNVVESLGVDIVAVGSGTSLNSETQELIRSLVERLERPVIIDGDGITAVAYDTSILKRRGAPTIITPHLVEFTRLTGLRPRDVEEDPVGVLRRACRELNSFIVLKGAHSLVGYPDGRVYINMTGNPGMAKAGSGDVLTGTIAAMYGIGLRDPGLATRMGVLVHGLAGDLAAEALGEDGVTPDDILNYLPKADPLLWRHAWPEHQADTHKFRRGAVAVLAGGVAGVGAPRLSARAALRVGAGLATLLCEPEALPVHAARGPDALMQRAVADRGALEAFLRDRRVSAVLAGPALGLDRRADDLLRAVIADDRPAVLDADALTALAGLPDGGVRLLGGRGGDTVLTPHEGEFQRLFGEEPAIAAEPSKLERARKAAALSGAVIVYKGADTVVAAPDGRGAINVTGSVALATAGSGDVLAGLVAGLLAQGMPAFEAGCAAVWLHGLAGEKLGMGLIADDLPEAIPPLLQD",
    "response#2": "LAGSPDMPGAAILASWAALTSGAGIVRLIHQEGMNAELSAAPLELVRQTYSPQDLEPVIEVMNKSSASFIGPGMGRTPDAFLVLKTLFKYLSKPCVIDADALTMLSEKEVPLPPSAVMTPHHGEMLRLLGKDKXYFSTEQLLQLAKNFAXRKKITLVLKGAPTFILQKGHPIYVNPRGSPGMATAGSGDVLTGMIAAFXAQHLQPIQAAIVAVYLHAVAGEYAALXKTEHCLIASDIV",
    "response#3": "VFCGEKEGAGIIAATAASRFGTGLTTLVVHEYISPPPFLMHATVVPETVSALAIGMGLGDHFEQEFLEKYVINSHLPIVLDADAFYSEELLAILSQKEREVVITPHPKEFSALWKQLTGESLSVAQIQAARFETVRRFNEKYPHVTLLLKGANMLLMQEEQLYINPLGSSKLSKGGSGDVLSGLIVSLLAQGYSGIEAAIQGSLALTLSAQHYTGASYAMLPTDIIDARN"
  },
  {
    "reference": "MTGITTEKPASTDAHRPARASAAGSPASLLDDVAGAIADIAAGRPVVVVDDEDRENEGDLVFAAEAATPELLAFTVRHTSGLICVPMLGEDLDRLQLPLMTADNQDGLGTAYTVTVDARTGVTTGISAADRARTVNLLAGADTESGDLARPGHVLPLRYRPGGVLTRRGHTEASVDLARLAGRRPAAVIAEVVQDDGAMARLPRLREFADEHGLRVISIEQLVHHVEATGTASAISAPAAPTSAPVARPPLVERVVETRIPNSHGQWRAVGYRGTADGAEHVALVLGDPTEGPDPLVRLHSECLTGDAFGSHRCDCGAQLEAAMGAIAAEGSGVIVYLRGHEGRGIGLLHKLQAYRLQDSGADTVDANLELGLPADARDFSAGAAVLADLGTYSVRLLSNNPKKTDGLSEHGVAVTERIPMPSAVTTDNLRYLQTKRDRMGHDLPGIVAAT",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be optimized for 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, magnesium ion binding, zinc ion binding activity in order to improve riboflavin biosynthetic process.2. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects.3. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile to enable effective catalysis of GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.4. The protein should be optimized for activity in Cofactor biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. The designed protein must have at least one GTP cyclohydrolase II domain. The designed protein sequence is ",
    "response#1": "RVASASLPTEHGPFIVTAYESLIDGQTHMALVRGEIGEGEKVLVRVHSQCLTGDVFGSARCDCGRQLLLAMERVANEGRGVLLYLNQEGRGIGLGNKIRAYELQDEGLDTVEANERLGFKPDQRDYGIGAQILGDLGISTMRLLTNNPRKFIGLEGYGLSILESVGGIVEDPEVTVHMPTRWGDFVCHAYTSPYGDNPGAVHIALVKGDISGDEPVLTRVHSECFTGDLLGSLRCDCGPQLHTAMSMIEKEGRGVLLYLRQEGRGIGLLAKLKAYDLQDKGLDTVEANLALGFAPDQRDYGIGAQILADLGLKKLRLMTNNPVKITGLSGYGLEITERVREVSASLPTKYGNFKIYGYSNKINNEQHIALIKGDIGDGKNLLCRVHSECLTGDTFGSLRCDCGQQFATAMKQIENEGRGVLLYMRQEGRGIGLINKLKAYALQDNGLDTLDANLALGFPGDMREYYIGAQILRDLGATTLRLLTNNPDKVYQLSEFGIKIIERVGGVQEYRVATNLPTIYGNFKLHLYRSYVDDYLHIALCHGDIGSMDGLPTPQHQEAVLVRVHDECLTGDIFGSLRCDCGEQLHNALKMIQKEGKGVVLYMRQEGRGIGLENKLHAYALQEKEGLDTVEANERLGFLPDKRDYGIGAQILKDLGITKMRLLTNNPKKFKALEGYGLEIVERG",
    "response#2": "GVDMPTAWGDFELIAYEQTNTQEIHMALIKGKWEKDEKVLVRVHSSCVTGDIFGSCRCDCGPQLHRAMEMVQEAGKGVVLYMNQEGRGIGLVNKLKAYKLQEEGMDTVQANLALGFPMDKRDYGVGAQILRDLGISKIRLISNNPTKRAGLLGYGLEIVDTVGEIVVYDRNLRVATNLPTIYGNFKLHLYRSYVDDYLHIALCHGDIGSMDGLPTPQHQEAVLVRVHDECLTGDIFGSLRCDCGEQLHNALKMIQKEGKGVVLYMRQEGRGIGLENKLHAYALQEKEGLDTVEANERLGFLPDKRDYGIGAQILKDLGITKMRLLTNNPKKFKALEGYGLEIVERRVSEAKLPTPMGTWQLIGYRTRDHSEEHVALVMGNVGGGEPTLVRVHSQCLTGDVFSSQRCDCGEQLEIAMRHVAEERRGVIVYMAQEGRGIGLHNKIAAYALQDQGMDTVEANLALGFAADRRDYGIGMQILRDLGVHRIRLMTNNPAKRHGLSGYGLEIVERVGEVEEERRVATNLPTIYGNFKLHLYRSYVDDYLHIALCHGDIGSMDGLPTPQHQEAVLVRVHDECLTGDIFGSLRCDCGEQLHNALKMIQKEGKGVVLYMRQEGRGIGLENKLHAYALQEKEGLDTVEANERLGFLPDKRDYGIGAQILKDLGITKMRLLTNNPKKFKALEGYGLEIVERGEVEEER",
    "response#3": "GVDMPTAWGDFELIAYEQTNTQEIHMALIKGKWEKDEKVLVRVHSSCVTGDIFGSCRCDCGPQLHRAMEMVQEAGKGVVLYMNQEGRGIGLVNKLKAYKLQEEGMDTVQANLALGFPMDKRDYGVGAQILRDLGISKIRLISNNPTKRAGLLGYGLEIVDTVSNAVMTDEGPCVAKAKMPTRYGEFTIYGYINKLNGEHHVALVKGDITDGEPVLCRVHSECLTGDALGSARCDCGQQYDAAMKMIAKEGRGVLLYMRQEGRGIGLINKIKAYQLQDQGLDTVEANLKLGFPEDARDYTIGTQILVDLGVRKMRLLTNNPLKVYGLSGYNLEIVERVEVTVHMPTRWGDFVCHAYTSPYGDNPGAVHIALVKGDISGDEPVLTRVHSECFTGDLLGSLRCDCGPQLHTAMSMIEKEGRGVLLYLRQEGRGIGLLAKLKAYDLQDKGLDTVEANLALGFAPDQRDYGIGAQILADLGLKKLRLMTNNPVKITGLSGYGLEITERVREVEIKLPTDYGEFKAIGYSNVVDGKEHVAIVKGEIGGSKPTLVRVHSECLTGDVFASKRCDCGPQLHAALAQIEEHGSGVVLYMRQEGRGIGLLNKMRAYKLQEEGYDTVQANEELGFAPDLRDYGIGAQILRDLGIRKMQLLTNNPRKITGLKGYDLEIVERLY"
  },
  {
    "reference": "MATKVAEIKTLKQGKYLVLGGEASKITSISTSSPGKHGAAKARIEAVGIFDNQKRSLVKPVNAKVDIPIIDKRVGQVLAIMGEEVQLMDLETYETIDLPIPEDLKGDIAEGKEVEYIEAMGNMKIMRTKGGN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Functions by promoting the formation of the first peptide bond.2. A protein that contributes to positive regulation of translational elongation, positive regulation of translational termination by functioning in cytoplasm with ribosome binding, RNA binding, translation initiation factor activity, translation elongation factor activity. The designed protein sequence is ",
    "response#1": "MQLIEKALDSRRREATLESGLEQLETSTAQMIEWLERMLHYVEDVNKNGEKPGDAQIGRQLMDIVTASSNNMQPEKLDTLVKNTLRDYVMVSYLAKLTQTQLQVHERCS",
    "response#2": "MQLIEKALDSRRREATLESGLEQLETSTAQMIEWLERMLHYVEDVNKNGEKPGDAQIGRQLMDIVTASSNNMQPEKLDTLVKNTLRDYVMVSYLAKLTQTQLQVHERC",
    "response#3": "MQLIEKALDSRRREATLESGLEQLETSTAQMIEWLERMLHYVEDVNKNGEKPGDAQIGRQLMDIVTASSNNMQPEKLDTLVKNTLRDYVMVSYLAKLTQTQLQVHERI"
  },
  {
    "reference": "MPALSRLPRASPGAGPPRGCSGSSGGAGGGAGPPRRGSPAALRLAKMNPRLRHWREAATLLLAAGTPGRPPRSPLGPFDYELLLLRRSSRSGFMPGAHVFPGGLAEPADFSAEWLGLLPASPHCGLGAVKPPPPGCSRAPIFATDRESLGSQLPGEVAFRICALRETFEEAGILLLVPGRGPAPPLPAERLPPAAELGEWRRRVREDASCFLRLCRHLGCAPNIWALHEWSNWLTPVGRAGPGGRRYDTAFYLCCLQQRPPHVSQDDQEVTAVLWSSPAEAIERFKSQEIWFPPPQFYEFCRLCNFSSLEELQQFSSARALEGCERWMPVMLSAADGAIQLLPGDELYPEDPDYTGEKKINLSTDKKVEDLMREGGVFHRMVMKDINNLAIYVNIQAKYKHINPLMIKCDNSDYNSRL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be optimized for metal ion binding. The designed protein sequence is ",
    "response#1": "LRTSRTNPAKRPDGLDTCIATLKKNGADALIAIGGDDTLSVATKLFDKGVRVVGVPKTIDNDLAGTDFTFGFDTAVNVVTEAIDRVHTTAEAHNRVMVVEVMGRDAGWIAIYSGIAGGADVILIPEQPFDVDKVAAKIRRRHERGRYFSIVVVAEGAKFASKNGPVMQSGETDEFGHARLGGIANTLALELEKRTGFETRTVVLGHIQRGGSPTAFDRVLATRYGLGAIDL",
    "response#2": "LRTSRTNPAKRPDGLDTCIATLKKNGADALIAIGGDDTLSVATKLFDKGVRVVGVPKTIDNDLAGTDFTFGFDTAVNVVTEAIDRVHTTAEAHNRVMVVEVMGRDAGWIAIYSGIAGGADVILIPEQPFDVDKVAAKIRRRHERGRYFSIVVVAEGAKFASKNGPVMQSGETDEFGHARLGGIANTLALELEKRTGFETRTVVLGHIQRGGSPTAFDRVLATRYGLGAIDALTAD",
    "response#3": "LRTSRTNPAKRPDGLDTCIATLKKNGADALIAIGGDDTLSVATKLFDKGVRVVGVPKTIDNDLAGTDFTFGFDTAVNVVTEAIDRVHTTAEAHNRVMVVEVMGRDAGWIAIYSGIAGGADVILIPEQPFDVDKVAAKIRRRHERGRYFSIVVVAEGAKFASKNGPVMQSGETDEFGHARLGGIANTLALELEKRTGFETRTVVLGHIQRGGSPTAFDRVLATRYGLGAIDAWVS"
  },
  {
    "reference": "MRTTYLAKPGEVERKWYIVDATDIPLGRLSTVVASILRGKNKPTFTPNVDTGDFVIVINAEKIKLTGRKATGKIYYHHTLHPGGIKSKTAGEMREKTPERLIETSIKGMLPHNTLGRKQALKLHVYAGSEHNQQAQNPEKLDITNLI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have structural constituent of ribosome within ribosome to regulate translation. The designed protein sequence is ",
    "response#1": "EIDVHVPIHLVGEAAGVKDGGVLQQAMHQLSIRVLPSNIPPSIDVDITNLQVGDVITVSHIQTNGAYEINHDPTEVIASILPPQ",
    "response#2": "EIDVHVPIHLVGEAAGVKDGGVLQQAMHQLSIRVLPSNIPPSIDVDITNLQVGDVITVSHIQTNGAYEINHDPTEVIASILPPQ",
    "response#3": "EIDVHVPIHLVGEAAGVKDGGVLQQAMHQLSIRVLPSNIPPSIDVDITNLQVGDVITVSHIQTNGAYEINHDPTEVIASILPPN"
  },
  {
    "reference": "QQIGSEDGEPPQQRVTGTLVLAVFSAVLGSLQFGYNIGVINAPQKVIEQSYNETWLGRQGPNGPGSIPPGTLTTLWALSVAIFSVGGMFSSFLLGIISQWLGRKKAMLFNNTLAVLAGALMGLAKAAASYEMLILGRFLIGAYSGLASGLVPMYVGEIAPTHLRGALGTLNQLA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that contributes to glucose transmembrane transport, cellular response to insulin stimulus by functioning in cytoplasmic vesicle membrane with glucose uniporter activity, hexose transmembrane transporter activity.2. The protein should have a Helical; Name=2, Helical; Name=5 domain that is long enough to span the entire membrane.3. The designed protein should contain Extracellular topological domains. The designed protein sequence is ",
    "response#1": "MLLFVSMAGWMFGADTGSIGGITNMRDFQSRFADRYNPVTDTYSYSSARQGLITGMVNVGSFFGCLISSPTTDRIGKRNSIIGFCVIYLIGVIIQVTAVPSWVQIMVAKIWTGLGIGALSVLAPGYQSEVAPPHIRGTIVVTYQLCVTAGIFIAACINMGTHKLYKTAQWRVSIGINLLWGIITLVGVSFLSESPRYLVSIGKDDEAIQVMCKNAELPPDSDIIQTEYKQIKSDCEAAVEGGPCTWPEIFGKEIRYRTFLGMAIMSFNQLTGCNYYFYYGTQVFKGTGMESPYLASLILDAVNFGCTFGGLFVLEYCGRRVPLIIGGVWQSICFFIYAAVGNRALTRKNGTSNHRAGAVMIVFSCLFIFSFAQTWGPAAYVIVGESYPIRYRSKCAAVATTSNWLWNFLISFFTPFITNSIGFKYGYIFASCNLCASIVTFLFAHESRRNAALT",
    "response#2": "MLLFVSMAGWMFGADTGSIGGITNMRDFQSRFADRYNPVTDTYSYSSARQGLITGMVNVGSFFGCLISSPTTDRIGKRNSIIGFCVIYLIGVIIQVTAVPSWVQIMVAKIWTGLGIGALSVLAPGYQSEVAPPHIRGTIVVTYQLCVTAGIFIAACINMGTHKLYKTAQWRVSIGINLLWGIITLVGVSFLSESPRYLVSIGKDDEAIQVMCKNAELPPDSDIIQTEYKQIKSDCEAAVEGGPCTWPEIFGKEIRYRTFLGMAIMSFNQLTGCNYYFYYGTQVFKGTGMESPYLASLILDAVNFGCTFGGLFVLEYCGRRVPLIIGGVWQSICFFIYAAVGNRALTRKNGTSNHRAGAVMIVFSCLFIFSFAQTWGPAAYVIVGESYPIRYRSKCAAVATTSNWLWNFLISFFTPFITNSIGFKYGYIFASCNLCASIVTFLFAHESE",
    "response#3": "MLLFVSMAGWMFGADTGSIGGITNMRDFQSRFADRYNPVTDTYSYSSARQGLITGMVNVGSFFGCLISSPTTDRIGKRNSIIGFCVIYLIGVIIQVTAVPSWVQIMVAKIWTGLGIGALSVLAPGYQSEVAPPHIRGTIVVTYQLCVTAGIFIAACINMGTHKLYKTAQWRVSIGINLLWGIITLVGVSFLSESPRYLVSIGKDDEAIQVMCKNAELPPDSDIIQTEYKQIKSDCEAAVEGGPCTWPEIFGKEIRYRTFLGMAIMSFNQLTGCNYYFYYGTQVFKGTGMESPYLASLILDAVNFGCTFGGLFVLEYCGRRVPLIIGGVWQSICFFIYAAVGNRALTRKNGTSNHRAGAVMIVFSCLFIFSFAQTWGPAAYVIVGESYPIRYRSKCAAVATTSNWLWNFLISFFTPFITNSIGFKYGYIFASCNLCASIVTFLFAHESKLRMYL"
  },
  {
    "reference": "MATILLLLLGLLVGLPLLRAHGVTGSAAPTPPPLPVLPVPSYAQLQWQLSEMALFLHFGPNTFTDSEWGSVRADPAVFAPSALDAGQWARAAAAGGFGRVVLTAKHHDGFCLWPSALTNYSVAASPWKGGAGDVVGELAAAARAEGIGLGLYLSPWDRHEPVYGDTVAYNEHYLGQMTELLTRYGDVEEVWLDGAKGEGKDMDYMFDAWFALIHQLQQRVVIFSDAGPDTRWVGDEAGVAGYTCWSPFNKSTVTIGHIIPEYSRCGDPFGQDWVPAECDVSIRPGWFWHASEKPKNATTLLDIYYKSVGRNCLLILNVPPNSSGLISTEDMQVLQEFTEIRQTIFSQNFAANATVTASTVRGGLGNQQFAPSNVLQESIYSYWAPEEGQSSWEMLFDLGQSASFNVIQLQEPIQMGQRVIKFRVEILVDELWQTIVEGTTIGYKRLFQFPVVEGQFLKLSIDGARADPLISFFGVFTDSFSVTYSLENHEKPSVVNSSEVIMLRTDHSFGNKSIATM",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be expressed specifically in the lysosome2. We need a protein that can perform an alpha-L-fucoside + H2O = an alcohol + L-fucose with high efficiency. The designed protein sequence is ",
    "response#1": "LSSGTWEILMVRSAQVDTSLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAPGADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKASELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWL",
    "response#2": "LSSGTWEILMVRSAQVDTSLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAPGADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKASELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWR",
    "response#3": "LSSGTWEILMVRSAQVDTSLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAPGADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKASELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWQ"
  },
  {
    "reference": "MNRILRISETHPLTWRLRDDQKTVWIKEYCDKNGYLSLKKALKEMLPEDVINIVKESGLKGRGGAGFSTGLKWSLMSQNHSYTRERGYLICNADEMEPGTYKDRLLIEKIPHQLIEGIILSAYALNVSRAYIFLRGEYVQAEHILKQSIQEAINFGFIGLNILGSNFNIELVLHTGAGRYICGEETALINSLEGRRANPRSKPPFPAVFGLWGKPTCVNNVETLSNVPCIILNGVSWYKNLSKSSDTGTKLMGFSGNVRNPGVWELPFGTTAREILEDYAHGMKSGFSLKAWQPGGAGTDFLLEEHLDLPMDFKNISQAGSRLGTALSMAVDNKTSMVSLVYNIEKFFSRESCGLCTPCRDGLPWIVKILKSLEQNKGHKNDVKNLEKLCSHLSPGKTFCAHAPGAIEPLQSAIKYFRSEFESGINIRKRDLNKKIIGIQSNCI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have a low Km value for a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) +   NAD(+).2. The protein can be expressed in respiratory chain complex I and perform NAD binding, 4 iron, 4 sulfur cluster binding, FMN binding, quinone binding, NADH dehydrogenase (ubiquinone) activity without triggering undesirable cellular responses.3. The protein design should incorporate a binding pocket that specifically recognizes [4Fe-4S] cluster to enhance enzymatic efficiency.4. For general function, the protein need meet that NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain; Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient .5. The NAD(+), FMN, [4Fe-4S] cluster binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "VKDSGLRGRGGAGFATGTKWSFIPQGDTGAAAKPHYLVVNADESEPGTCKDIPLMLATPHVLIEGVIIAAYAIRAHHAFVYVRGEVVPVLRRLHNAVAEAYAAGFLGRNIGGSGFDLELVVHAGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVAGLYGCPTVINNVETIASVGEIHIQICGTTTCMICGAEDLIRVCKEKIAPEPHALSADGRFSWEEVECLGACTNAPMAQIGKDFYEDLTVEKLAALIDAE",
    "response#2": "VKDSGLRGRGGAGFATGTKWSFIPQGDTGAAAKPHYLVVNADESEPGTCKDIPLMLATPHVLIEGVIIAAYAIRAHHAFVYVRGEVVPVLRRLHNAVAEAYAAGFLGRNIGGSGFDLELVVHAGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVAGLYGCPTVINNVETIASVGQAIKAVRRWTEFYKHESCGKCTPCREGTFWLDKIYERLETGRGSHEDIDKLLDISDSILGKSFCALGDGAASPVMSSIKHFRDEYLAEGKNPAKLKWHIQICGTTTCMICGAEDLIRVCKEKIAPEPHALSADGRFSWEEVECLGACTNAPMAQIGKDFYEDLTVEKLAALIE",
    "response#3": "VKDSGLRGRGGAGFATGTKWSFIPQGDTGAAAKPHYLVVNADESEPGTCKDIPLMLATPHVLIEGVIIAAYAIRAHHAFVYVRGEVVPVLRRLHNAVAEAYAAGFLGRNIGGSGFDLELVVHAGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVAGLYGCPTVINNVETIASVGNTCFGQCCAIEMMATNASNYDLERFGIFPRSSPRQSDLMIVAGTVSMKMAERVVRLYEQMPEPRYVLSMGSCSNCGGPYWEHGYHVLKGVDRVIPVDVYVPGCPPRPEALIGGLMIGRLGDECCAIEFIATSMSRHDFMRLGVIPFAHGPRQADLMVVSGTVTDKMAPAIKRLYDQMPEPKYVISFGACSNCGGPYWDSYSVTKGVDQLIPVDVYVPGCPPRPEALLHGILKRMDKLGVTTVKQLRAAPIQRLETLLGSEAAPFWLRLRGIDNRQVDVRSEQLSGHVTRPGQYEAPLGITLRELLDYAGGVRAGHRLKFWTPGGSSHRQSITLPAEYSDEAALRFLLRFQEEQLDPQQIRRISLTADRFYQTGEQHISLSDFAK"
  },
  {
    "reference": "MKKIVFASRNRGKIEEIRTLLAESGVALKSLDDYPDLPDILEDGKSFLENALKKARAISELTRETVLADDSGLEVAALDGAPGIYSARYAGDSADDGMNISKLLSKLEGVPPEDREAAFRCILVLYRPDGRYDVFDGMWEGRIAEAPAGKGGFGYDPVFYLPERGVTAAELPAGVKNRISHRAMAAEKLKVWLQKGTHENGA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..2. The designed protein must be optimized for dITP diphosphatase activity.3. The designed protein should have a binding site that is specific to the target substrate ligand. The designed protein sequence is ",
    "response#1": "DIIP",
    "response#2": "ISPTE",
    "response#3": "ITDPXS"
  },
  {
    "reference": "MRIVLGVGGGIAAYKAALLLRLFTEGGHQVTVVPTEAALKFVGAPTWEALSGHPVQTDVWSNITDVPHVRIGQQADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWDHPATVANVATLRGRGVSVVEPESGRLTGADTGPGRLPDPEAIHAAAMAALEASTGVAQVPADLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGVALAETAVARGAHVTLVAANVDLPMPEGVDVVRVGTARELQQEITSRTAEHDVVVMVAAVADFRPAEYADSKIKKTHDPGDPDAAPTITLVRNPDILAGIVADRGAATTPLIVGFAAETGDATGSVLDLARAKLARKGCDLLVVNEVGVDKTFGLDETSVHVLTRGSEAVTDLGPASKSEVSHGIWDVVARSLTVD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should interact with enzymes and substrates within the Cofactor biosynthesis, coenzyme A biosynthesis pathway to enhance or regulate its activity.2. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A3. The designed protein must be able to perform phosphopantothenoylcysteine decarboxylase activity, FMN binding, phosphopantothenate--cysteine ligase activity under specific conditions.4. The protein requires Mg(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "MRIIVGVAGGIAAYKACEVVSRLVKAGHSVRVVMTPAAAQFVAPLTFRALSGHAVGIEAADEPAGPLSHVQLAHWAEALVIAPATASLMSRLAHGQTDDLLGLVYLGIRGPVVAAPAMEPEMWSHPRTQANASVLRADGVRLVGPAVGRMASGQEGLGRMAEPAEIVAALEDALGTTRKLAGKRVVITAGPTREPLDPVRFVSNRSSGKMGYALAATAAELGAAVTLVSGPTALPDPVAVDTVRVETAEEMLDAVQERAADADLFVGCAAVSDHKPAERLDRKRKGKGGFALELTPNPDILAAVAALDNGPVTLGFAAETHDALAHGREKRAAKGADWLAVNDVTEADAGFEADTNRVTLIDAAGNVEDWPTLSKDEVAERLVMRIAEAL",
    "response#2": "MRIIVGVAGGIAAYKACEVVSRLVKAGHSVRVVMTPAAAQFVAPLTFRALSGHAVGIEAADEPAGPLSHVQLAHWAEALVIAPATASLMSRLAHGQTDDLLGLVYLGIRGPVVAAPAMEPEMWSHPRTQANASVLRADGVRLVGPAVGRMASGQEGLGRMAEPAEIVAALEDALGAGRGLPLAGKRVVITAGPTREPLDPVRFVSNRSSGKMGYALAATAAELGAAVTLVSGPTALPDPVAVDTVRVETAEEMLDAVQERAADADLFVGCAAVSDHKPAERLDRKRKGKGGFALELTPNPDILAAVAALDNGPVTLGFAAETHDALAHGREKRAAKGADWLAVNDVTEADAGFEADTNRVTLITADGVEDWPRMGKDEVAEALAQRIADALGG",
    "response#3": "MRIIVGVAGGIAAYKACEVVSRLVKAGHSVRVVMTPAAAQFVAPLTFRALSGHAVGIEAADEPAGPLSHVQLAHWAEALVIAPATASLMSRLAHGQTDDLLGLVYLGIRGPVVAAPAMEPEMWSHPRTQANASVLRADGVRLVGPAVGRMASGQEGLGRMAEPAEIVAALEDALAAA"
  },
  {
    "reference": "MQTLDPVTPALLPLRRVTRPVQVGALTIGGNAPVVVQSMINEDTLDTPGAVAAIRALHKAGCELVRVTVPSLAHARALERIRKTLEDTYRPVPLVADVHHDGGPIALEVARHVDKVRINPGLFVFRRARLGDYTAEDVERARAQIREELKPLVDVLGAQDKAMRIGVNHGSLSERMLYIHGDTPEGMVQSAVEFVEICEQLGYHNLVISLKASRVPVMIAVYRLAAKLLDARGMHYPFHLGVTEAGDGEYGRIKSTAGIATLLADGIGDTIRVSLTEDPLKEIPVCYGILQALGLRRTMVEYVACPSCGRTLFNLETVLHRVREATRHLTGLNIAVMGCIVNGPGEMADADYGYVGKTPGTISLYRGREEIKKVPEARGVEALVDLIKSDGRWVDP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. We require a protein that can specifically bind to the metabolites produced in the isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 5/6, isopentenyl diphosphate biosynthesis via DXP pathway pathway.2. The protein must exhibit the following characteristics: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME- 2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate..3. The protein must exhibit 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity. The designed protein sequence is ",
    "response#1": "MLIGFGYDVHRLVPGRPLVLGGVEIPSKRGLEGHSDADVALHALMDALLGAAGLGDIGTHFPPTDPAWAGANSLDLLRRVVGLLAEEGYRPVNCDITVVAERPKIGPYVPAMRERIGAVLGVEPRRVSIKATTNEQMGFVGREEGIAAYAVALL",
    "response#2": "MLIGFGYDVHRLVPGRPLVLGGVEIPSKRGLEGHSDADVALHALMDALLGAAGLGDIGTHFPPTDPAWAGANSLDLLRRVVGLLAEEGYRPVNCDITVVAERPKIGPYVPAMRERIGAVLGVEPRRVSIKATTNEQMGFVGREEGIAAYAVALL",
    "response#3": "MLIGFGYDVHRLVPGRPLVLGGVEIPSKRGLEGHSDADVALHALMDALLGAAGLGDIGTHFPPTDPAWAGANSLDLLRRVVGLLAEEGYRPVNCDITVVAERPKIGPYVPAMRERIGAVLGVEPRRVSIKATTNEQMGFVGREEGIAAYAVALL"
  },
  {
    "reference": "AIAAVITFLILFTIFGNALVILAVLTSRSLRAPQNLFLVSLAAADILVATLIIPFSLANELLGYWYFRRTWCEVYLALDVLFCTSSIVHLCAISLDRYWAVSRALEYNSKRTPRRIKCIILTVWLIAAVISLPPLIYKGDQGPQPRGRPQCKLNQEAWYILASSIGSFFAPCLIMILVYLRIYLIAKRSHCRGPRAKGAPGKGESKQTGQASLGAPSSAKLPNLVSRLVAAREANRHSKSTGEKVEGETPEGPGTPGVPPSWPPLPSSGRGQEEDIYRASPEEEAGDDEEEECEPQAVPVSPASACSPPLQQPQGSRVLATLRGQVLLSRGVGTASGQWWRRRAQLTREKRFTFVLAVVIGVFVLCWFPFFFSYSLGAICPQHCKVPHGLF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be able to regulate regulation of smooth muscle contraction. The designed protein sequence is ",
    "response#1": "PLFETTLKSRAVSEDSDVRFTCIVTGYPEPEVTWYKDDIELDRYCGLPKYEITHQGNRHTLQLYRCQEEDAAIYQASARNTKGIVSCSGVLEVGTMV",
    "response#2": "LKSRAVSEDSDVRFTCIVTGYPEPEVTWYKDDIELDRYCGLPKYEITHQGNRHTLQLYRCQEEDAAIYQASARNTKGIVSCSGVLEVC",
    "response#3": "VEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIAAPSVATGAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKY"
  },
  {
    "reference": "MTSFLTADRVEQQSSPTQSAQDTRRLRLFSGSSNPHLAREIAAYLGVPDGPCVIKRFADGELYIQIQESIRGCDVFLIQPTCAPVNDHLMELLIMVDACRRASARQITAVIPYYGYARADRKTAGRESITAKLVANLLVKSGVDRVLAMDLHSSQIQGYFDIPCDHIYGSPVLVDYLRTRNLGEVVVVSPDVGGVARARAFAKQMNDAPLAIIDKRRSGHNVAESLTVIGDVADKTAVLIDDMIDTGGTIYQGAKLLRERGAARVLACATHAVFSPPAVERLSTPGLFEEVVVTNSIPLVPERSFPQLQVLSVANMLGEAIWRIHEESSVSSMFR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.2. The designed protein should be able to compete with endogenous proteins or inhibitors for D-ribose 5-phosphate, ATP binding.3. A protein that can perform magnesium ion binding, ribose phosphate diphosphokinase activity, kinase activity in cytoplasm to support phosphorylation, ribonucleoside monophosphate biosynthetic process.4. The designed protein should contain one or more Ribose-phosphate pyrophosphokinase N-terminal domains that are essential for its function.5. The protein must exhibit the following characteristics: Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P)..6. We require a protein that can specifically bind to the metabolites produced in the 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1, 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, Metabolic intermediate biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGADRALSMDLHAPQIQGFFNVPVDNIFATPVLIEDIKRQRFENYVVVSPDVGGVVRARAIAKRLDDADLAIIDKRRPKANVSQVMHIIGDVRGKTCVLVDDIVDTSGTLCKAANALKEHGAEHVVAYITHPVLSGPAIENIEASSLDELVVCDTIPLSEENRKIRKINVAPLLGEAIKRIHTGLSISAMFR",
    "response#2": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGVDRALTMDLHAPQIQGFFNVPVDNLFATPVLIEDIKRQRFENYVVVSPDVGGVVRARAIAKRLDDADLAIIDKRRPKANVSQVMHIIGDVRGKTCVLVDDIVDTSGTLCKAANALKEHGAEHVVAYITHPVLSGPAIENIEASSLDELVVCDSIPIPESKKLGNLKILSVAPLLARAIREVFEDGSVTSLFD",
    "response#3": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGADRIMSVDLHAAQIQGFFNKPVDHLATPVLIEDIKRQRFENYVVVSPDVGGVVRARAIAKRLDDADLAIIDKRRPKANVSQVMHIIGDVRGKTCVLVDDIVDTSGTLCKAANALKEHGAEHVVAYITHPVLSGPAIENIEASSLDELVVCDTVPLPPEKSLHKLTILSIAPLLGEAIRRIHTGLSVGAMFE"
  },
  {
    "reference": "MSNLMAIILAAGKGTRMKSALPKVLHQVGGKPMVQQVLDAAKFAGADKNVVIIGFGAQAVADALAGQAEFAVQTEQLGTGHAVMQARKELADFDGTVMVLCGDTPLLTGELLRTLYTEHANAQAAATVLTAIMPDPAGYGRVIRNPAGQVVKIVEHKDASLVELAVSEINTGIYCFEAKELLQALDGLTCNNAQGEYYLTDVVAILNSQGQKVWAVKAPDYHETLGINSRVQLAEAEQLIRRRKLDLLMESGVTIMDPNSTFIDSQVEIGPDTIIYPFTWIEGNTKIGTNVAIGPNSRITNTVIGDNVTIHFTYAHDCTIGDQVTVGPYVHLRPQTELKNGVKIGNFVEVKNTVVGEKSKIPHLSYIGDTDMGAGVNIGSGTITVNYDGKNKNRTTIEDQAFIGCNTNLVAPVTVGHGSYVAAGSTITKNVPPGSLGVARARQNNIEGWAEKQRR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain..2. The designed protein should be able to compete with endogenous proteins or inhibitors for acetyl-CoA, UDP-N-acetyl-alpha-D-glucosamine, Mg(2+) binding.3. The presence of MobA-like NTP transferase domains is critical for the interaction of the designed protein with its intended targets.4. We require a protein that can specifically bind to the metabolites produced in the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway. The designed protein sequence is ",
    "response#1": "MTAILAAGRGTRMKSNLPKVLHSLGGRSLIGRVIDSVKPLSPTRQIVVVGYQAVEVQAAMESIPDLEFVEQTVQLGTGHAIQQLLPHLEGYTGDLLVLYGDVPLLRTETLKNLLQTHTQHQNAATILTLTMPDPTGYGRILRNENGSVVGIVEQKDASESELEITEINSGVYVFRAADLKKYIPKLNSDNAQKELYLTDVIGLLRSEDRPVRSFLTSDPLEGLGVNDRVQLAQAEAILRERINTRWMREGVTIIDPIATYIDATVQVGQDTIIYPGTFLEGHTIIAAGCTLGPGTTLRDCQVGEGSQIIHTVALESQIGAGCQVGPFTYLRPGTILEDRTKVGDFVELKATRIGTGSKVPHLTYLGDTTVGTGVNIGAGTITCNYDGQKKWPTVIEDGAFIGSNSNLVAPVRVGAGALVGAGSTITEDVPAGSLALARGRQTNIEGWVEKNRRGRAAAEAARRARENREE",
    "response#2": "MGSELPKVMHPLAGRPMVQHLMNTVMGLDPDKVVVVVGPGMDQVAQAVAPAQTVVQAERLGTGHAVLQARAALGDFDGDVLVLYGDTPLITPDTLRRMLAERRGAKNPAVVVLGFRPDDPGPYGRLVMAGGDLVAIREEKDATEAERAVSLCNGGLMAIRGDVALQLLDKVGNDNAAGEYYLPDVVALAVADGRRCAVVETGEEEVAGVNSRAELASVEAAWQRRRRAELMTAGVTLIDPASITIDDTVEIEPDVVIEPQTHLRGNTVIQTGSRIGPGSLIENSHLGENVTLMYSVVKNSIVKAGSTLGPYTHLRGDVEVGASCRVGNFVELKNTKLGDRTNVAHLSYLGDTTAGSRVNIGAGTITANYDGVKKHPTKIGDRTKTGSNSVLVAPVTLGDDVYVAAGSTVTEDVPDDSLVIARARQVNKPGWAARFRAAQAAKK",
    "response#3": "VVVLAAGEGKRMKSALPKVLHPLLGRTLLGHVLTAAEPLAADRTVVVVGHGADQVRAHLTEVAPQADAVLQAEQLGTGHAVRIALDAVADAAGTVVVINGDVPLLRPETVAALVEAHEGAGAAATVLAAEVPDPTGLGRIVRDAAGRLERIVEEKDATEQERAIREINSGIYAFDAALLRDGLKRLTTDNAQGEEYLTDVLGLLRADGAAVGAHRAADWIEIQGVNDRVQLARLRAELNRRTLERWMREGVTLLDPARFDLRGELIHGRDVVLDANVIIEGKVELGDRVRIGAGCVIKNSVIGDDCEISPYSVVEDAVLEQKCTIGPFARLRPGAELAEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGAGVNIGAGTITCNYDGANKHKTIIGDDVFVGSDTQLVAPVSVASGATIGAGTTVTRDIAQNELVLSRVPQRNVEGWVVRKRPGSAAAQAAERARRAEVSGAPAGEGPAGAGS"
  },
  {
    "reference": "MSNVSLVILCAGNSSRFNQSTKKQWLRVDNEPIWLYVSKKLTSYYSFDKIVVTSSKYELNYMQNFCDDITFVEGGDTRQKSIKNALKHIDSDFVMITDVARVNIPSCVVTNLIDSKNEADCIVPVLDVSDTVIYENNTINRDNVKLIQTPQLSNTKILNQALKTDIEFTDDSSAIKNINGQVKYVKGSIKSKKITFSEDLEVIKELPSPSNNFFTGTGFDIHPFEDNKKMFLGGVEIDVDYGFKAHSDGDVLIHSVIDALLGAIGAGDIGEFFPDTDEKYKGVDSKILLEEIVQFVKNVGYEIINLDLTIIAQKPKINPYKQEIKSKIASLLKIEKQFVNIKATTAEKLGFIGRSEGVAVQSVATVKYYDWKKQI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must possess metal ion binding, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity to support terpenoid biosynthetic process, isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway under certain conditions.2. The designed protein should interact with enzymes and substrates within the Isoprenoid biosynthesis, isopentenyl diphosphate biosynthesis via DXP pathway, isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6 pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "GRHDFTDDASIAEWAGLPVTLAEGSVDNIKLTVKRDMAMADEKLSRFVVPDVRTGNGYDVHQLEPGDGVTLCGVFIPHHLKLSGHSDADVALHALTDALLATCGAGDIGDHFPPTDMRWRGAASTIFLEHAAKLVRDRGGVITNADVSLIAEAPKIAPHRDQMRKNLADTLDITIDRCSVKATTNEKMGFVGRNEGIAAIATATVIEW",
    "response#2": "LRTGTGFDVHRFGDGDHVWLCGVRIAHDRSLVGHSDADVGLHALTDAVLGAIADGDIGQHFPPSDPQWAGAASHIFLREAARRVRQRDGRIVNVDVTILCEAPKIAPHREAMRSAIAGILEISLDRVSVKATTTEGLGFTGRREGIAAMATATVLELVDG",
    "response#3": "RTGSGFDVHRFGQGDHVTLCGVRVPHERGLQGHSDADVGMHTITDAIYGALAAGDIGRHFPPSDPQWKGADSAIFLRHAGALAKDRGYRIGNIDCTLICERPKIGPHAIEMQSVLAEVLGIEEGRISVKATTSERLGFTGREEGIAAMATVTLETLAAPAVPTAVGHGYDVHRLVEGRRLVLGGVEIPYALGLLGHSDADVLAHAVGDAVLGACGLGDLGRHFPDSDPRWKDASSLDLLRRIGELAAEAGYRVLRVDATLVAQRPKVAPHVPRMEANLSGGLGLPPGSVTVKATTTEGLGFEGRGEGISAHAVALVTP"
  },
  {
    "reference": "MKRFAKNIVIYLVIFGLVLGAAVLYKGGAGASNKKVRYSTLVQYLEQGKITEISIGSDYKVTGKIGDKEYVYAYCNNIVDEQWLFQKYITKGISSGKISYYSKPPSSGSAILQILPTIIMVIALGFLFYLMMNQGGNGKAFQFGKSKARLYKGDGKVITFADVAGLKEEKEELEEVVDFLKDPRKYQEVGARIPKGILLVGPPGTGKTYVSRATAGEAGVPFFTISGSDFVEMFVGVGASRVRDLFEQAKKNAPCIVFIDEIDAVGRQRGAGLGGGNDEREQTLNQLLVEMDGFGENSGIIILAATNRPDVLDPALLRPGRFDRQIVIGIPDVKGREEIIRVHSKDKPLSEEVSPEILARRTPGFTPADLENLLNEAALITARRNGRRIRMAEIEEATTKVIAGPAKKSRVISEKEKRLTAYHEAGHAIVMRSIPGSDPVHQITIIPRGQAGGFTMSLPTEDRSYGTKKDMEHEIQHLLGGRVAEALTLDDISTGASNDIQRATETARDMVTKYGFSSKIGPVNYSDSSDVFLGRDYSKTKSYSEGVAMEIDTEVRRIIEEAYSMTEKILQENMDKLERVAQALMRVETLDGEQFEALYTGKVTAEELEQQVMEKEAEIRRQNAREAEESERLRREEEARLAAELARYDTDYLNDDDEPDEPEVQSTPAQSEPEPEAESPEQDAPEAQDAPRRTEENNPEGENSADEKEGERL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must have a active site that is structurally stable and resistant to denaturation.2. Ensure the presence of a signal peptide within the protein.3. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP, Zn(2+) binding.4. The protein must exhibit the following characteristics: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins; Plays a role in the quality control of integral membrane proteins..5. The protein should have a Helical domain that is long enough to span the entire membrane.6. A protein that contributes to protein catabolic process by functioning in plasma membrane with ATP hydrolysis activity, metalloendopeptidase activity, zinc ion binding, ATP-dependent peptidase activity, ATP binding.7. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.8. The presence of AAA+ ATPase domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MVMSEKEKRNTAFHESGHAIVGRSVPEFDKLYKITIVPRGRALGLTQFLPEEDRYSMSRVSLKSQIMTLFGGRIAEEMVNGPDGVTTGASNDIERASQLARNMVTKWGLSDRMGPLLYDEEEREVFLGMSAGSQPKSISAETAREIDEEVRKIIDDCYAAAKAILTEHEDKLHKMAEALVELETLTPEQADKASRLLAN",
    "response#2": "MISQKERQRVAFHEAGHSICGLVLSDSRTVRKVTIVPHGRTGGYNLMMPKDDQFLLTKKQLMEQIVGLMGGRAGEEVVVGDQSTGASNDFEQATAIAHSMVVNYGMTDSLGMVELEKEGETNPYGLKPYSEATSAKIDEAVREILDEAHTQAVEIVKNNREKHRIIAEALLKYETLDEKQIMRLQARFR",
    "response#3": "IPRGTLMVGSPGTGKTLLARAIAGEAKVPFFSISGSDFVEMFVGVGASRVRDMFEQAKKQAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFEANDGIIVIAATNRPDVLDPALLRPGRFDRQVVVPLPEYIFEPEDIDLNVIARRTIGFTPADLENLMNESALLSAREDLKEIPMRLLDFVDLKAIAQRTPGFSGADLENLLNEAALVAARADKKKIGMVEIDRAIGARNYTVWRQKGTGRARVGFEGGQMPLYRRIPTRFRGIINPAYAAELPAVVSGAEL"
  },
  {
    "reference": "MLKIGSHVSMSGKKMLLAASEEAVSYGANTFMIYTGAPQNTRRKAIEELNIEAGHEHMKAHGIEDIVVHAPYIINIGNTKNPDTFALGVNFLRAEIERTEAIGAKQLVLHPGAHVGAGAEAGLRQIIRGLNEVLTREQTVEIALETMAGKGSECGRTFEELAYIIDGVAYNDKLSVCFDTCHTHDAGYDIVNDFDGVLSEFDRVIGLERLKVLHINDSKNPRGSRKDRHENIGFGHIGFRALNYIVHHPQLADIPKILETPYVGEDKNNKKPPYKHEIAMLRAQTFDDQLLEKIVADTQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must be optimized for zinc ion binding, deoxyribonuclease IV (phage-T4-induced) activity, DNA binding. The designed protein sequence is ",
    "response#1": "VSSGCDVIQIFTQNSNQWKGKAVSPADAQLFRDKLAASGLSHVMSHDIYLINLAAAPGEVKDKSLIAFKEEMQRCAALGIGKIVMHPGSHTGDGEDTGIRRICEAFDQLFGEVPQFTGKVLLENTAGQGTNLGYRFDHLKAIIEGSSYPTRFGVCFDTCHAFASGYPIADRDGYRRTFDEFDRALGIDKLMAFHLNDSKKGLGCKVDRHEHIGAGALGLEPFRFILNDPHFKLVPKFIETPKG",
    "response#2": "SYARELKAEAVQVFVANPRGWATPAGNPKQDEAFREACAAGSVPAYVHAPYLINFGSHTGATVERSVESLRHSLRRGRAIGALGVVVHTGSATGGRERPVALKQVREHMLPLLDELTHDDDPYLLLESTAGQGASLCSRTWDFGPYFEALDAHPKLGVCLDTCHIFAAGHDLTGPSGMHQTLDLLVDTVGEGRLRLIHANDSKDVAGAHKDRHENIGAGHIGEDPFRALMTHPATDGVPLVIETPGGKEGHAADVARLKR",
    "response#3": "ATSIGAKAFALFTKNQRQWKAPKLTTSSIEAFKKNCEAGGFRPEHILPHDSYLINLGSPDPEKLQRAREAFIEEMQRAEELGLLLLNFHPGSHLKEIAEERCLELIAESINRALDATKTVTAVIENTAGQGTNLGNRFEQLAFLVDRIEDKTRVGVCLDTCHLFASGYDLQTTAAIESTFMEFDRIVGLRYLRGMHLNDAMQPLGSRIDRHAGIGKGTIGMDAFRWIMNNPACEEIPLILETPDSAAWSEEISLLN"
  },
  {
    "reference": "MNERLKRVENALAVLKEGGKVLLCDHESRENEGDVIVAAEFATPEAIQFMAQKACGLICLALDEQYIEKFRLNPMAPKNNSRHQTAFTVSIGARKGTTTGISAHDRSHTVLTVVDDASNPEDIVSPGHLFPLKAEKHGVFVREGHTEGSVDLMRLAGLKPAAVICEVMGEDGHMAKGDALVNFAKAHKLPVLSVADVLAYRLFHEARAEYIAQANLPTHEFGAFKVHAFKDRITGVDHLAIVAGELASIKKSLVRVHSSCVTGDIFSSQRCDCGEQLAVALDKIQAEGGVLLYLNQEGRGIGLANKIKAYALQEQGLDTIEANHQLGFPADMRSFSIAADMLKHLGVKKIALMTNNPRKLEELSDYGLEVVERVPLEIDAHENNKAYLKAKKQKLGHFLSRA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..2. The designed protein must have at least one GTP cyclohydrolase II domain.3. A protein that can modulate the overall flux of metabolites through the riboflavin biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway, enhancing or suppressing its activity as needed.4. A protein with 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, zinc ion binding, manganese ion binding, GTP binding is needed to enhance riboflavin biosynthetic process in a specific context.5. The designed protein should have a high specificity for Mg(2+), Zn(2+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "ERAAETRLPTAHGEFRAIGYRSTLDGVEHIALVAGDIGAGEDVLVRVHSECLTGDVFGSLRCDCGPQLAAALETVADNGRGVVLYMRGHEGRGIGLLHKLQAYQLQDNGHDTVDANLELGLPADARDYGTGAQILSDLGVRSMRLLTNNPAKRAGLEGYGLEILERVPNVTLTRTIDDSRLAETVLPTAIAGEFKAIVYESEVDPNHHLALVKGEIDSEQPILVRVHSQCLTGDVFGSLRCDCGSQLHTAMKMIEKEGKGVLVYMRQEGRGIGFANKMKAYALQDAGHDTVEANLALGFKPDLRNYGIGAQILRDLGVRKMRLMTNNPKKIVGLEGYGLEVVERIREAEAKLPSAFGDFRIVAYTNELDGKEHVAIVKGEIDSSKPVLVRVHSECLTGDIFGSHRCDCGPQLHAALRMIEAEGRGIVLYMRQEGRGIGLINKLKAYRLQEQGYDTVEANKKLGFPADLRDYGIGAQILLDLGVKQMRLMTNNPRKIRGLSGYGMQVAERVREAEAKLPSAFGDFRIVAYTNELDGKEHVAIVKGEIDSSKPVLVRVHSECLTGDIFGSHRCDCGPQLHAALRMIEAEGRGIVLYMRQEGRGIGLINKLKAYRLQEQGYDTVEANKKLGFPADLRDYGIGAQILLDLGVKQMRLMTNNPRKIRGLSGYGMQVAERVVSRIDERIATTRLPTMHGDFRAYGYRDLVGGGEHLALVKGELSGDDPVLARIHSECLTGDAFGSLRCDCGPQLQAALAAVAKAGRGVVVYLRGHEGRGIGLAHKLQAYELQDNGHDTVDANIELGLPADARDYGVGAQILADLGVRSLALMTNNPAKLTGIEGYGLDVAER",
    "response#2": "GVDMPTAWGDFELIAYEQTNTQEIHMALIKGKWEKDEKVLVRVHSSCVTGDIFGSCRCDCGPQLHRAMEMVQEAGKGVVLYMNQEGRGIGLVNKLKAYKLQEEGMDTVQANLALGFPMDKRDYGVGAQILRDLGISKIRLISNNPTKRAGLLGYGLEIVDTVPGMNRVPRLAETVLPTAIAGEFKAIVYESEVDPNHHLALVKGEIDSEQPILVRVHSQCLTGDVFGSLRCDCGSQLHTAMKMIEKEGKGVLVYMRQEGRGIGFANKMKAYALQDAGHDTVEANLALGFKPDLRNYGIGAQILRDLGVRKMRLMTNNPKKIVGLEGYGLEVVERIERVAQTRIPTRHGDFRAVGYRCTVDGSEHVALVRGEPGTTPDAAGEPLVRLHSECLTGDAFGSLRCDCGPQLDRALATVAADGAGVVVYLRGHEGRGIGLLAKLSAYALQDTGHDTVEANLALGLPVDARDYWIGAQILSDLGVDRMRLLTNNPAKRAGLEAYGLTVTATGNKVWIE",
    "response#3": "MRTRLAETVLPTAIAGEFKAIVYESEVDPNHHLALVKGEIDSEQPILVRVHSQCLTGDVFGSLRCDCGSQLHTAMKMIEKEGKGVLVYMRQEGRGIGFANKMKAYALQDAGHDTVEANLALGFKPDLRNYGIGAQILRDLGVRKMRLMTNNPKKIVGLEGYGLEVVERILVDASGARERVAQTRIPTRHGDFRAVGYRCTVDGSEHVALVRGEPGTTPDAAGEPLVRLHSECLTGDAFGSLRCDCGPQLDRALATVAADGAGVVVYLRGHEGRGIGLLAKLSAYALQDTGHDTVEANLALGLPVDARDYWIGAQILSDLGVDRMRLLTNNPAKRAGLEAYGLTVTATSYRRPRF"
  },
  {
    "reference": "MQKRGREVSCLLISLTAICLVVTPGSRVCPRRCACYVPTEVHCTFRYLTSIPDGIPANVERVNLGYNSLTRLTENDFSGLSRLELLMLHSNGIHRVSDKTFSGLQSLQVLKMSYNKVQIIEKDTLYGLRSLTRLHLDHNNIEFINPEAFYGLTLLRLVHLEGNRLTKLHPDTFVSLSYLQIFKTSFIKYLYLSDNFLTSLPKEMVSSMPNLESLYLHGNPWTCDCHLKWLSEWMQGNPDIIKCKKERIPSSPQQCPLCMNPRISKGRSIAMVPSGSFLCTKPTIDPSLKSKSLGIQEDNGSASVSPQDFIEPFGSLSLNMTDLSGNKANVICSIQKPSRTLPIAFTEENDYIMLNMSFSTNLVCSVNYNHIQPVWQLLALYSDSPLILERKPQHTETPLLSPKYQQVALRPEDTFTNIEADFKADPFWFQQEKISLQLNRTATTLSTLQIQFSTDAQITLPKAEMRPVKRKWTMILMMNNTRLEHTVLVGGTIALDCPGKGDPSPHLEWVLADGSKVRAPYVSEDGRILIDKKGKLELQMADTFDAGLYHCISTNDADADILTYRITVVEPYVENKHENGALHTVIMGEILDLPCLSTGIPDASISWILPRNTVFSQSSRDMQILNNGTLRILQATPKDQGHYRCVAANPSGADFSSFQVSVQMKGQRTIEHDRDIDGSGLEEPKPSVLLKQPPSLKLPASSLTGTEAGKQVSGIHKKNKHRDLTHRRRGDSTLRRFREHRRQLPLSARRIDPQHWAALLEKAKKNSV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein contains novel LRRCT, Ig-like C2-type, LRRNT domains that confer a unique function or activity.2. Target a Polar residues compositional bias in the protein's composition for improved properties.3. The protein should be optimized for stability and solubility when expressed in extracellular region where it will be involved in ossification, regulation of neuron migration.4. The protein must exhibit the following characteristics: Involved in the control of early migration of neurons expressing gonadotropin-releasing hormone (GNRH neurons).5. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "LSIITYVGCLISALASICTIFFLYFRSKQRDQITSMHIHMNLLVAIFLLDITFLISEHLAASSEAICRAGGLFLHFSLLSCLTWMGIEGYNLYRLVIEVFNAYHDHLLLKLCLVGWGFPFLCVMLIFLAGWTNYGPFSVPIYESIDGRSTSATICWITSPLIHNIVNLGFFSLVFLFNSVMLGAMVREILRQNKKGHKLKHVLALLGLSILLGIPWALIFFSFTSGVFCLVSLYIFTIINSLQGFLIFLWYWTMNRELRQAYFAIFK",
    "response#2": "PALIRKSGPRWVIAEEGGHAVFSCSGDGDPAPTVSWMRPQGAWLGRVGRVRVLEDGTLEIRSVQLRDRGAYVCVVSNVAGNDSLRTWLEVV",
    "response#3": "LSIITYVGCLISALASICTIFFLYFRSKQRDQITSMHIHMNLLVAIFLLDITFLISEHLAASSEAICRAGGLFLHFSLLSCLTWMGIEGYNLYRLVIEVFNAYHDHLLLKLCLVGWGFPFLCVMLIFLAGWTNYGPFSVPIYESIDGRSTSATICWITSPLIHNIVNLGFFSLVFLFNSVMLGAMVREILRQNKKGHKLKHVLALLGLSILLGIPWALIFFSFTSGVFCLVSLYIFTIINSLQGFLIFLWYWTMNKELQKQLRCSWV"
  },
  {
    "reference": "MQNFLPFSRPAIGSDEINAVVNVLGSGWITTGPQNQQLETDFCAAFGCKHAVAVCSATAGMHITLMALGIGPGDEVITPSQTWVSTINMIVLLGAEPVMVDIDRDTLMVNAADIEAAITSKTKAIIPVHYAGAPCDLDALRQIAQQYKIPLIEDAAHAVGTRYGDQWVGEKGTTIFSFHAIKNITCAEGGLIATDDDELAAKVRRLKFHGLGVDAFDRQIQGRSPQAEVVEPGYKYNLSDIHAAIAVVQLQRLPEINARRQALAARYHQALVDSPLQPLFVPDYAHQHAWHLFMVRVDKERCGIDRDSLMARLKEVGIGSGLHFRAAHTQKYYRERYPSLHLPNTEWNSARLCTLPLFPDMLDSDVDRVVDALATIIGPHRV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The stability of the protein-pyridoxal 5'-phosphate complex should be optimized to maximize enzymatic activity.2. For general function, the protein need meet that Catalyzes the conversion of UDP-4-keto-arabinose (UDP-Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N)3. The protein design should be able to enhance the efficiency of lipopolysaccharide biosynthetic process, response to antibiotic.4. The protein should have a fast turnover rate for 2-oxoglutarate + UDP-4-amino-4-deoxy-beta-L-arabinose = L-   glutamate + UDP-beta-L-threo-pentopyranos-4-ulose.5. The designed protein should interact with enzymes and substrates within the Bacterial outer membrane biogenesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "VLILGVNGFIGNHLTERLLKDGNYDIYGMDIGSSAIERFIGNPRFHFIEGDVSIHTEWIEYHIKKCDVILPLVAIATPIEYTRNPLRVFELDFEENLKIVRYCVKYNKRIIFPSTSEVYGMCDDKEFDEDNSRLIVGPINKQRWIYSVSKQLLDRVIWAYGAKEGLKFTLFRPFNWMGPRLDNLNSARIGSSRAITQLILNLVEGSPIKLVDGGEQKRCFTDINDGIEALFRIIENRDNKCDGQIINIGTGSGYSVLDMVKAFEKVTDRTVPHKLVDRRPGDIATCYADSSLAEKELGWKAQRGLEEMCRDAWKWQQQNPTGYRD",
    "response#2": "VTLSPSVTVGNEAPFTLFGGINVLESRDLALRAAEEYVRVTQKLGIPYVFKGSFDKANRSSIHSYRGPGLEEGMKILQAVKDTFGVPVITDVHEPWQAAPVAEVADVVQLPAFLARQTDLVEALAKTGRAVNIKKPQFMSPAQIQNVVEKFVEAGNEQLILCDRGTCLGYDNLVVDMLGFGVMKKVSGNRPVIFDVTHSLQQRESGAAASGGRRAQVAELARAGMAVGLAGLFLEAHPNPAEAKCDGPSALPLAQLEPFLAQVKAIDDLVNGGLEWR",
    "response#3": "VTLSPSVTVGNEAPFTLFGGINVLESRDLALRAAEEYVRVTQKLGIPYVFKGSFDKANRSSIHSYRGPGLEEGMKILQAVKDTFGVPVITDVHEPWQAAPVAEVADVVQLPAFLARQTDLVEALAKTGRAVNIKKPQFMSPAQIQNVVEKFVEAGNEQLILCDRGTCLGYDNLVVDMLGFGVMKKVSGNRPVIFDVTHSLQQRESGAAASGGRRAQVAELARAGMAVGLAGLFLEAHPNPAEAKCDGPSALPLAQLEPFLAQVKAIDDLVERYRQPPQA"
  },
  {
    "reference": "MKRDYYEILGVARNATPEEIKKAYRKLARKYHPDVNKDDPNAAEKFKEINEAYEVLSDPEKRARYDQFGHAGVDGNFAGQGGFGGGAGINFEDIFSGFGGFGDLFDMVFGSGRKARQGPVPGDDIEAVLELTLEEAVFGGEKELRVTRTETCGHCHGNGAEPGTPIITCPTCQGRGQIHQEVKTLFGRMVRSQVCSTCRGEGKIPKTPCRECGGSGLVRKTRSITVKIPPGIDHGHRLRIAGGGEAGRFGGPPGDLYVYIKIKPHKLFKREDIHLKLEKEISFVQAALGAKVEIPTIDGGTEILEIPEGTQTGTVFTIKGKGVPVVNGSGRGNLYVTVKVVTPTKLTERQKQLLREFEEISKQKEETFKEKFNKFKNKFAL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.2. The protein must exhibit the following characteristics: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion; Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.3. The presence of J domains is critical for the interaction of the designed protein with its intended targets.4. The designed protein should have zinc ion binding, unfolded protein binding, heat shock protein binding within cytoplasm to regulate protein folding, response to heat. The designed protein sequence is ",
    "response#1": "VGVDISFFEMTNGCTRTIEYTKRVTCSACDGFGAEGGETGMVSCNSCEGNGFILKNQRSIFGTVQSQMLCQSCGGQGKQAKHKCKTCKGSKYKKVPTTKEIQIPAGIYHGDALVDDHGGNEFGGHVGKLVVHVGVLPSKIFKRVDHNVVANVLVDPMIAITGGKIELPTLEGIKEFNLRAGTKSGEQIVIPGGGIKFTKNFRKKAGDLIIIISYKAS",
    "response#2": "LRYDVEVSLEDAFKGIKVPISYVTNVKCSSCSGIGSEGAVNSVKCGNCNGAGSVRTRKGFLTIEEVCNVCNGEGEVIKNKCRRCGGSGRVRNEVSLLVTVPKGIETGNKVRVNGKGEAGFRGAQEGDLYVYIRVKEHKFFTRRSSDLHCSVPIKMTIAALGGEIEMPSIDGSWTKLKIPEGTQSGDQIRMRGKGMPEVNSKDRRGDMYVHVTVETG",
    "response#3": "RFDLKLKFREAVFGGEQQIRISHLESCKTCEGTGAKPGTRPQTCSTCQGSGQVRRMTRTPLGNFTQVSVCPTCNGQGQVVTEKCDSCGGRGQNQVSKKLQIKIPAGVDTGTRLRVSNEGDAGQKGGPPGDLYVYLFVQEDSEFRRDGTNVLSEFKISYLQAILGAQLPVKTVDGEETITIPAGTQPGTVLTLENHGVPRLGNPVSRGDHLITVIIDIK"
  },
  {
    "reference": "MTTVTVTTEIPPRDKMEDNSALYESTSAHIIEETEYVKKIRTTLQKIRTQMFKDEIRHDSTNHKLDAKHCGNLQQGSDSEMDPSCCSLDLLMKKIKGKDLQLLEMNKENEVLKIKLQASREAGAAALRNVAQRLFENYQTQSEEVRKKQEDSKQLLQVNKLEKEQKLKQHVENLNQVAEKLEEKHSQITELENLVQRMEKEKRTLLERKLSLENKLLQLKSSATYGKSCQDLQREISILQEQISHLQFVIHSQHQNLRSVIQEMEGLKNNLKEQDKRIENLREKVNILEAQNKELKTQVALSSETPRTKVSKAVSTSELKTEGVSPYLMLIRLRK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be able to modulate intracellular signal transduction in a way that leads to a desirable outcome.2. For general function, the protein need meet that Centriolar protein required for centriole subdistal appendage assembly and microtubule anchoring in interphase cells The designed protein sequence is ",
    "response#1": "LDAATESNEIELQLRVLVLEHRKDLGLTTVWDDQLSYLLSPALASYEIERTTGISAGNEEFQDCIRRAVPDGHTFKGFPIHFVHRNARRAFATCLRSPFCDEIISCRGDQMRLAVRVRVYTYPEAACAVWIMFACKYL",
    "response#2": "LDAATESNEIELQLRVLVLEHRKDLGLTTVWDDQLSYLLSPALASYEIERTTGISAGNEEFQDCIRRAVPDGHTFKGFPIHFVHRNARRAFATCLRSPFCDEIISCRGDQMRLAVRVRVYTYPEAACAVWIMFACKYV",
    "response#3": "LDAATESNEIELQLRVLVLEHRKDLGLTTVWDDQLSYLLSPALASYEIERTTGISAGNEEFQDCIRRAVPDGHTFKGFPIHFVHRNARRAFATCLRSPFCDEIISCRGDQMRLAVRVRVYTYPEAACAVWIMFACKYR"
  },
  {
    "reference": "MSSNTTTPTPTSTPTPTSSPSIGPVPIPYESASFGNLLFFAISLVLCGVVVLIGMKQYISLKRTPIYATFFSFLGWFMCFSIAYLVPLDIIVTDHLQCVIENNETLTGECEIPVTYLPYGMITQQWRFLYFGSLILCWVIFPVLQSFSTAGDFRFWERVKRATKENVILYTFMFIAGLIGIIVILSVKEMDPSSFLSFVMLLANVYGVILITITMGYGLIDVPRNLLRKGSHYAILRNYRVEAVVLKTELEDVKRQLIDHLKLIKTISDRAGQYDPFRIYLDVIISKCPTEYDVLIQEYHAEPLPAGMEAEILSYKYLVGIHSTLLDLVDRNHSAEVLYERLLGKAFAIEDIIETRERNKQTAGNNQIAGEERSIQWSFKSTKSNGKFEYLWHMYIHPWYFIIAGLVCVCLSGIILWSEIVLALVSNPDYSPFYRAIVRMEPGIGLQIFCFIPMIYMCVCSYSTLFKLRISNYYRLVPQQSNTFSIMFSANYLCRLAAPLAYNFIQICHVNQDNSIVSPFSKIMGDMNAFGDNALGKRFTLFFPIFMIVVCVISFFNLHKRLAGSCCIRSLRIVTDTSEGAVDHGLKILKQEREERSLTGGVAPVKTRMSIVKEMFTKKKGAGILVTDNSQLDGASGAHREPSIDSSNRYKPTPTKTSINIPKLSRTYTSAAGNVYSSTQDGDENSNSGIKPINSFFSSILGEKGNASNNNNNNNNNNNNNNSNNKNSNNNNNSILTSNYESYSTPRTKDKQGLLSSALDKMDFSFQD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be optimized for stability and expression in cell periphery, plasma membrane, membrane, filamentous actin, ruffle where it will participate in cell migration, pseudopodium organization, chemotaxis.2. The protein must contain a signal peptide for proper functionality. The designed protein sequence is ",
    "response#1": "LMNDFWEMETISFPPDGSSITPAHHYNDFRFKVYAPIAFRYFRDLFGIAPDDFLMSMCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLWPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNSLMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGKQVL",
    "response#2": "MGFSSELCSPQGHGAVQQMQEAELRLLEGMRKWMAQRVKSDREYAGLLHHMSLQDGGGRGTGPYSPISQSWAEITSQTEGLSRLLRQHAEDLNSGPLSKLGLLIRERQQLRKTYSEQWQQLQQELTKVRHQTQHRALLRVEFCAVTPQATHKDRDKAKDKYVRSLWKLFAHHNRYVLGVRAAQLHHHHHHQLMLPGLLQSLQDLHQEMACILKEILQEYLEISSLVQDEVVAIHLEMAAAVARIQPEAE",
    "response#3": "MCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLWPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNSLMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGL"
  },
  {
    "reference": "MKKRCEFLIFGKVQGVGFRRFVKYRVDKLNEESKVLSGNVCNLSDGSVRVIAQGEEEALEKLCKILEIGPIKSEVERIQSREIDIDESLNDFEILR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein contains novel Acylphosphatase-like domains that confer a unique function or activity.2. The protein must have high specificity towards acylphosphatase activity. The designed protein sequence is ",
    "response#1": "TLHLQIEGRVQGVWFRESMRREAERLGVDGWVRNRPDGSVEAVVQGTDEAVAALVAWAKMGPPLAHVERVDLSETEGEYSGFEKRC",
    "response#2": "RFDVSGWEHLQTEGPYVIISNHQSSLDVLGLMEILPDRCTMIAKKELIYAGTVGLVCWLGGIVFINRKKTNDAKNVMADAAKTMLDDQIRLWVFPEGTRNQRGGLLPFKKGAFHLAVQAQVPIIPIVFH",
    "response#3": "RFDVSGWEHLQTEGPYVIISNHQSSLDVLGLMEILPDRCTMIAKKELIYAGTVGLVCWLGGIVFINRKKTNDAKNVMADAAKTMLDDQIRLWVFPEGTRNQRGGLLPFKKGAFHLAVQAQVPIIPIVFR"
  },
  {
    "reference": "MPFKFDSIQDALADLAAGRAIVVVDDENRENEGDVICAAQFATPDLINFMAVEARGLICLAMTGDRLDELDLPLMVASSSFEDENEQTAFTISIDAATQWGVTTGISAEDRARTIQVAINAKAQPSDLRRPGHIFPLRAKKGGVLKRAGHTEAGVDLARLAGLYPAGVICEISNADGSMARLPDLVSYVQRHHLKLISIADLISYRLRHERFVKREAIAAMPTKFGDFQVYAYRNTLDGSEHLALVKGDPSTFKDEPIMVRMHSECLTGDALGSLRCDCRMQLQATLKMIENAGKGVVVYLRQEGRGIGLVNKLKAYSLQDMGLDTVEANERLGLPVDQRNYGMGAQILMDLGIGRFRLITNNPRKIAGLKGYGLDMVDRVPLLIEATSYNASYLATKAKKMGHLLLQTYLVTVAIRWREADSLTADPNAADPVTARYERLEKLRALGQKHDLLLQEEARSIAVAVFGQLPLIAHLGLDQPDLAGDRWYKDPHHPYVQAISALLDELIAWPDIDHLEFLVSDGGDPFTQLKVRLDQQIFPWALSGPNAVTPGDVCQQLKTQRIYVFSQHNISA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein have GTP cyclohydrolase II domains with specific sequence motifs that are crucial for its activity.2. For general function, the protein need meet that Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.3. The protein with the reaction GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate, the Proton acceptor; for GTP cyclohydrolase activity, Nucleophile; for GTP cyclohydrolase activity must be optimized to ensure efficient catalysis.4. The presence of Zn(2+) is necessary for the protein to function in the desired environment.5. A protein with manganese ion binding, magnesium ion binding, GTP cyclohydrolase II activity, GTP binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity is needed to enhance riboflavin biosynthetic process in a specific context.6. A protein that is capable of catalyzing reactions within the Cofactor biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4, riboflavin biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MSLKEILANAGVTYTSXTXLDXQIXDVAXDSRQVQPGTLFVAVRGAMTDGHLFVXDAVKRGAVAIVSEIEXGXPEXGXTPXVIVKDTRXAMSXAAAXFFGXPXDKXIMVAXVTGTNGKTTTTFLIESVFKKAGXRAGXIGTLGXRIGXEXLPTTHTTPDAIELHRTLAXMRXXGVSHCAMEVSSHALDQGRVDGCTFDVGVFTNLTRDHLDYHGTMENYFAAKAKLFTPEYLKEKAIINIDDPYGRRLAEQSXGXVITIGFSEGADVRAMXIKSSHSGIQXDLXTPWGXVXVXSXLXGXFNVXNILAATAAGXALGXDLDXIAAGIATVEGVPXRVEKXGXSAGXDXGXGXGXVXDXSHNAXSMKAALXALXGXTXKXTXAXGXKXXVXAAXGGXDXXAXDWQXIAETXAKXFDXVVXFGXARDNQXGRGXSYEXVLKALXDHXXXGXTQXEXKXHXDXGXAXG",
    "response#2": "VSVQMPTKYGNFELIAFKQVNTGEIHMALKKGEWEKGEPVLVRVHSSCVTGDILHSLRCDCGEQLAAAMKMVEKEGKGLILYMNQEGRGIGLMNKLKAYKLQEEGRDTVEANLELGFQMDERDYGVGAQILRHLNICKLRLITNNPRKRAGLLGYGLEVVENVEPADVYSFVKLPTEHGEFEFIPFRQTSNGLEHAALVKGKWTRDEPVLVRLHSSCFTGDIFGSLRCDCGSQLHKAMEMVEAAGKGVVIYLSQEGRGIGLFNKIKAYKLQDQGMDTVQANIKLGFGEDERDYGVGASIMRELGLGKIRLISNNPVKRAGLEGYGIKIVEAVPNSAYPEETDETREIEIQLPSEFGDFKAVGYTNSLDSYEHVALVKGEILEDEPVLVRVHSECLTGDVFGSFRCDCGPQLHAALSQIEAAGNGVLLYMRQEGRGIGLINKMRAYKLQEKGYDTVEANEQLGFAPDLRDYGIGAQILKDLGIKKMKLLTNNPRKIAGLEGYGLEIVDRVPASAYPEETDETKVAEAQLPSRYGGDWKAIAFENDIDKLDHLALVKGDINTRVPVLVRVHSECLTGDVLGSLRCDCGEQLHQAMTMIERAGKGVILYMRQEGRGIGLINKLKAYELQDQGRDTVEANLELGFKADLRDYGIGAQILLSLGIHKMRLMTNNPKKLIGLEGYGIDIVERPASAYPEETDEVERAVETRLPTKFGDFTAIGYRHSVGGQEHLALVAGKVEDLAGARDVLVRVHSECLTGDIFGSRRCDCGPQLEAALGAVSAAGRGVVVYIRGHEGRGIGLINKLKAYQLQDSGLDTVDANLEQGLPEDSREYSAAGQILRDLGLKSINLVTNNPHKGEGLEGFGVEVVART",
    "response#3": "ERAVETRLPTKFGDFTAIGYRHSVGGQEHLALVAGKVEDLAGARDVLVRVHSECLTGDIFGSRRCDCGPQLEAALGAVSAAGRGVVVYIRGHEGRGIGLINKLKAYQLQDSGLDTVDANLEQGLPEDSREYSAAGQILRDLGLKSINLVTNNPHKGEGLEGFGVEVVARTAATVSPGGASREIEIQLPSEFGDFKAVGYTNSLDSYEHVALVKGEILEDEPVLVRVHSECLTGDVFGSFRCDCGPQLHAALSQIEAAGNGVLLYMRQEGRGIGLINKMRAYKLQEKGYDTVEANEQLGFAPDLRDYGIGAQILKDLGIKKMKLLTNNPRKIAGLEGYGLEIVDRVPATAVQIGDEAVLRASGRVPSEHGPFTAITYRSPADDCEHVALVLGEVNQAGAEPGSAESAPVLVRVHSECLTGDVFASRRCDCGEQLAVAMTRIGEAGRGVVVYLRGHEGRGIGLSHKLRAYNLQDAGLDTVDANLAQGLPVDSREYGIGAQILRDLGVREIRLMTNNPAKYRGLAGHGVRVATVRDVVSGDDGKPLERAVETRLPTKFGDFTAIGYRHSVGGQEHLALVAGKVEDLAGARDVLVRVHSECLTGDIFGSRRCDCGPQLEAALGAVSAAGRGVVVYIRGHEGRGIGLINKLKAYQLQDSGLDTVDANLEQGLPEDSREYSAAGQILRDLGLKSINLVTNNPHKGEGLEGFGVEVVARTPAAITM"
  },
  {
    "reference": "MWDGFSNSFWSRDYITGINRAQRLINEGVEQNEKLLQLLHIRAKSASSCSDLLFKSFSKFNKHHPLNEESNDLPSDAAIHQLYESYLMEASLHKKLGEQLNILVINPFANWSKKYSRRVDEIVSAAIVKINNYNSRFSHVNSKKSNLTDRKPIPTSRKSNKSDSLASALSQLDINPSNVNKFDGLINIVDHPYTAEEFANVLLKLMKASTVKRKSYSHLGDYELVTNCSLLFEAIKTTFGLEKDSYVVKAGNQLIQHGLIRLLGIRRVFENDPEIDAQFTTKSQQLLKSYHLSILEVDPYFQVNDPITATNDDPVLQKYNLAYDNLEQCRHELELYLFMVFKDLEQAELDRLNAVKSVLVECSNYSGNFIPSLNSIFIDNLNSFKNLDSLRDMSTQINKHYTGYFIPVSNNELSTKDEYLFLQKSSLTEDNLIVSLVPKILAYLLDAYSYERDEEVLSCVWTTEVPLKDAFDLKSVLRKTDNVESVLNACVEKYTLSSITCSLRLCLLEFPDSLIRSSFYDYFKAIYTTYTDFEDLDHRLYSIKKCLLHLHSTPLHILEEIIRHLSAYAISIRMKDGQIRHLAKIISPCVLRPPDDLNIIPVEDTHPTLLVIDLINEFENLFADLERPSTPPVEIERALTPITTSPQKLKLPRSSSPCKNPSPTRRFRPF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The ideal protein would be one that plays a critical role in small GTPase mediated signal transduction, cytoskeleton organization through GTPase activator activity within cell division site, division septum, cell cortex, cytoplasm, plasma membrane.2. The designed protein should contain one or more F-BAR, Rho-GAP domains that are essential for its function.3. The designed protein's functionality will benefit from Polar residues, Basic and acidic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "EDLSVLLNREGSTLPWFIVRAFQAIRNHIATEEGLFRLSGTKRIIYEYKQKIDEGKEYNLSEILDIHVVCNLVKLYLRELQPEPLLTYSRYNELIDTCNIEDQNQRVDKISKILSSLPKHYYTLLHHLIHLLSQIASQPKSKMGPANLATVIGPNILICQTDVVLEDIALGNMVVTTIIQNFDRIL",
    "response#2": "EDLSVLLNREGSTLPWFIVRAFQAIRNHIATEEGLFRLSGTKRIIYEYKQKIDEGKEYNLSEILDIHVVCNLVKLYLRELQPEPLLTYSRYNELIDTCNIEDQNQRVDKISKILSSLPKHYYTLLHHLIHLLSQIASQPKSKMGPANLATVIGPNILICQTDVVLEDIALGNMVVTTIIQNFDRIV",
    "response#3": "QEVPQVLKSCAEFVEEYGVVDGIYRLSGVSSNIQKLRQEFESERKPDLRRDVYLQDIHCVSSLCKAYFRELPDPLLTYRLYDKFAEAVGVQLEPERLVKILEVLRELPVPNYRTLEFLMRHLVHMASFSAQTNMHARNLAIVWAPNLLRSKDIEASGFNGTAAFMEVRVQSIVVEFILTHVQ"
  },
  {
    "reference": "MAISAALVKELRERTGAGMMDCKKALEETNGDIELAIDNMRKSGAAKAAKKAGNIAAEGTILIKQGAGFTALLEVNCQTDFVAKDSNFLAFANQVLEVAAAGKVSVEDLKAQFEEARVALVAKIGENINVRRVEYIDGENVAAYRHGDRIGVVVTGTADEETLKHLAMHVAASKPEYVNPEDVPAEVVAKEREVQVEIAINEGKPKEIAEKMVEGRMKKFTGEVSLTGQPFIMEPKKTVGEFLKEKGATVANFIRLEVGEGIEKKEEDFAAEVAKQIAASQKA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP; It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.2. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining translation elongation factor activity. The designed protein sequence is ",
    "response#1": "AFVKDIAMHIAAASPQYVRREEVPAELLEREKEIYRAKARETGKPENIIEKIIEGQINKFYAEICLMEQNFVKDPDKTVQQFLNETISSIGENMSVRRFARFVLGL",
    "response#2": "AFVKDIAMHIAAASPQYVRREEVPAELLEREKEIYRAKARETGKPENIIEKIIEGQINKFYAEICLMEQNFVKDPDKTVQQFLNETISSIGENMSVRRFARFVLGE",
    "response#3": "AFVKDIAMHIAAASPQYVRREEVPAELLEREKEIYRAKARETGKPENIIEKIIEGQINKFYAEICLMEQNFVKDPDKTVQQFLNETISSIGENMSVRRFARFVLGD"
  },
  {
    "reference": "MRVLNDTISKVINYKFTDYAILEEALTHPSVNKRNSEDQVVSYERLEFLGDSVLNMVVSVMLFKMFPEEKEGALAKRKTDLVCGSTIANVAKEIELGNFIIMNNSERCNGGKCNLKNLENSLEALIGAIYIDGGLESVEKFIIRHWEKLAKDILDPPQDPKTSLQEWTQRNKLPLPKYELVKQTGPAHNPEFTISVCIESYDKVSACAPSKKIAEQKAAELILEKIKKTT",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must bind to Mg(2+) in order to perform its enzymatic function.2. The active site active site of the designed protein must be optimized for substrate binding and specificity.3. The protein design must be able to perform rRNA binding in order to contribute to rRNA processing.4. For general function, the protein need meet that Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon; Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S); Digests double-stranded RNA The designed protein sequence is ",
    "response#1": "MAAIEQRLGHTFADISLLTTAFTHVSALKSSRGRCDSYQRLEFLGDHVLGLVVSDMLYRAFPKADEGELSKRLADLVRKETCIDVAKSLDLQEGVKLGAVGAGAGARLRRSVLGDICEAVIGAVYLDGGRRDIETILCRA",
    "response#2": "MAAIEQRLGHTFADISLLTTAFTHVSALKSSRGRCDSYQRLEFLGDHVLGLVVSDMLYRAFPKADEGELSKRLADLVRKETCIDVAKSLDLQEGVKLGAVGAGAGARLRRSVLGDICEAVIGAVYLDGGHERLRALLDD",
    "response#3": "MAAIEQRLGHTFADISLLTTAFTHVSALKSSRGRCDSYQRLEFLGDHVLGLVVSDMLYRAFPKADEGELSKRLADLVRKETCIDVAKSLDLQEGVKLGAVGAGAGARLRRSVLGDICEAVIGAVYLDGGHED"
  },
  {
    "reference": "VRSFEKAPEKAPEKASEEVQREKYSLEGNTYQEQSPVLPDKPVGQNSVPTQRALKLHPKFSKKFQANEKPRDKAIHQARLRHFCRICGNPFKSDGHNRRYPVHGPVDAKTQSLFRKKEKRVTSWPDLIARVFRIDVKADMDSIHPTEFCHNCWSIMYRKFSSAPCEVYFPRNAIMEWHPHTPSCDICLTAHRGLKRKKRHQPNVQLSKKLKTVLNQARQDRQRKRAQSRVSSKEVMQKISNCSKIHLSTKLLAVDFPAHFVKSISCQICEHILADPVETSCKHVFCRICILRCLKVMGSYCPSCRYPCFPTDLESPVKSFLNILNSLMVKCPAQECNEEVSLEKYNHHVSGHKESKEALVHINKGGRPRQHLLSLTRRAQKHRLRELKIQVKEFADKEEGGDVKSVCLTLFLLALRARNEHRQADELEAIMQGKGSGLQPAVCLAIRVNTFLSCSQYHKMYRTVKAITGRQIFQPLHALRNAEKVLLPGYHPFEWQPPLKNVSSRTDVGIIDGLSGLASSVDDYPVDTIAKRFRYDSALVSALMDMEEDILEGMRSQDLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXILSPLIAEREAMKSSELMLEMGGILRTFKFIFRGTGYDEKLVREVEGLEASGSVYICTLCDATRLEASQNLVFHSITRSHAENLERYEVWRSNPYHESVEELRDRVKGVSAKPFIETVPSID",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should contain one or more RING-type domains that are essential for its function.2. A protein with NBD capability for targeted gene regulation.3. The designed protein should have protein homodimerization activity, zinc ion binding, sequence-specific DNA binding, endonuclease activity within nucleus to regulate histone monoubiquitination, B cell differentiation, V(D)J recombination, T cell differentiation in thymus.4. The protein must exhibit the following characteristics: V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T- lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments; RAG2 is not a catalytic component but is required for all known catalytic activities; Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination.5. Target a Basic and acidic residues, Polar residues compositional bias in the protein's composition for improved properties.6. The protein must bind to Mn(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "LRRMGCKATRWPEVMLKVFKVDLSEDMETIHPPMFCHRCWIVAMRGGGICSFSRTRVPVWKPHNTHCLLCYPKRHIHQRRGRKSSKPLHGAHSLPKRAKRDSSTSTGCNRVWRQTTDNPAAVGLKAWVKPAAQRSLWVKNITHCQKAHLTSNLLPGDLPKDFVSAE",
    "response#2": "RSMPAEIQHPYTKFSEWKFKLFRVRSFEKTSSNDLQEENKVQVEESLEKVSEVKLSAEIQSLEHTEPSFAALRCQPQAHEKTDNNPVDLKTTENEQSHVVNLQQLCRICGISFKSDPHNRSYPVHGPVDEETQGILRKKEKRATSWPDLISKVFKIDVRGDMDTIHPTRFCHNCWSIINQKFNNAPCEVYFPRNDAVDWQPHSGTCSVCTSVQRGIKRKSQLQGNSQLSKKLKRGTEHIKKRKAVKKNSQLKTNKTLIPKLTNCQKIHLSTKLLVVDYPADFMKSSFCSVCQICEHILADPVETSCKHLFCRICILRCLKVMGSYCPSCRRHSIPDELCHPFSKFSDWKFKLFRVRSMEKAPLPSEKEPLSATSSGDSAMEGAGLPGSVMKLCLGGKSKENIEGPSRHIDLKLQEIDTHMNHIKSLCRLCGMLLRKAKGPEHEVQGLLDEASRFSLRRMGCKALNWPEVILKVFKVDVAGDMETIHPPSFCHRCWTAAIRGGGFCSFTRTQVPEWMPHSTHCPLCFPKKVSLQRKGRKRRKALRGAPSLAKRSKLESHSSSAVGGGGRGSLGRTRVKKGLWVKNFTFCHKEHLSVQLLPDDLPLDFVSASASGFSQKSPTRSSVPAELHYPHSEFSQWKFKLFRVKSMERAPLPSETQPEKGALLGISPPTAPNIEADNGAGPGSVMKLCLGGKSKDSVEGPGRRVDKKLQEMDTHMNNLRSLCRLCGMMLRKVKGPVHDVQGELDEVSKGALRKMGCKLKRWSEVIIKVFKVDVTEDTEAVHPLSFCHRCWMVAIRGGGICSFSKIRVPEWKPHSSPCHLCSPRKPSFQRTGRKRRKAIPRAQSLAKRTKWDHSDGTGERRTLRPHVLRSWRKPSTQREQWVRNITHCQKDHLSTKLITEKLPVDFLL",
    "response#3": "MNCKFLSWPEVILKVFKVDVTEDTESVHPLSFCHRCWVIAIRGGGVCSFSKTNVPEWNPHSSPCHLCSPKKPSFKRTGRKRRLAIPRAQSLAKRSRRDHGDSTAGGERRALRQFADHYHGPVLRGWRKPTIQREQWVRNITHCQKDHLNTKLISEKLPVDFLFGGRPRQHLLSLTRRAQKHRLRELKLQVKAFADKEEGGDVKSVCLTLFLLALRARNEHRQADELEALMRKEAEMKVGEHGDSPSVSSSIMKLCLGGKNKENVEGAGRQVDLKLQEIDTHMNHLRSLCRLCGILLRKVKGPEHDVQGDLDESSRYPLRRMGCKATRWPDVILKVFKVDVSGDMESIHPPMFCHRCWTVAMRGGGICSFSRTRVPIWKPHNSHCLHCYPKRRTLQRRGRKRGKPLHSLPKRAKRESSTGGNRVWRQSAENPPAIGVRAWVKPATQRGLWVKNITHCQKDHLTSNLLPGDFPKDFXSAL"
  },
  {
    "reference": "MGEQTDQCFEEHGNDGKGSRTVVMSTGVIGQRLPIRKIINSIPAAHSNLGQTHDHWLKAARAICTTDTFPKLISRPFTLPSSPGKTYHLAGMTKGAGMIHPNMATLLGILCTDAPITPPALQALLSQSIAKSFNSISIDGDTSTNDTVALLANGAAGGSTIDLPSESASSESRANFDAMQTTLTNFAADLAKLVVRDGEGATKFVTIRVVNALNDTDAHLVASSISRSPLVKTALYGKDANWGRILCATGYTAGLSSTEDSVIPAQTSVSFVPTDGSPELKLLVDGEPESVDEARAAEILQMEDLEILVRLGKDGQGRDGECRFWTCDFSHEYVTINGDYRT",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate..2. The protein should be optimized for activity in L-arginine biosynthesis, N(2)-acetyl- L-ornithine from L-glutamate: step 1/4, Amino-acid biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The designed protein should be involved in arginine biosynthetic process by localizing to mitochondrial matrix and exhibiting glutamate N-acetyltransferase activity, methione N-acyltransferase activity, acetyl-CoA:L-glutamate N-acetyltransferase activity.4. The protein should exhibit Basic and acidic residues compositional bias.5. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile to enable effective catalysis of acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate.6. The designed protein should have a binding site that is specific to the target substrate ligand. The designed protein sequence is ",
    "response#1": "MNTRCVIKYGGAAMVKESLKKSFCDDIGLLRSVGLRPVVVHGGGPEITRTLEKLGGKAEFIDGQRVTNASDVKVVEMVLTGSINTELVTLLNQEGVAHAVGVSGVTRINKDFLEMLLQQGYVPIISPVGLGEDGQSYNINADTVAAEVAIAIGAQKLIYLSDVPGILKGGELIGQLTTKDVEALIADGTISGGMIAKARSILKVLASGVPNVHGMSGNGRIERPSEMAAALAAATAETEPPLALSA",
    "response#2": "MARCVIKYGGAAMVKESLKKSFCDDIGLLRSVGLRPVVVHGGGPEITRTLEKLGGKAEFIDGQRVTNASDVKVVEMVLTGSINTELVTLLNQEGVAHAVGVSGVTRINKDFLEMLLQQGYVPIISPVGLGEDGQSYNINADTVAAEVAIAIGAQKLIYLSDVPGILKGGELIGQLTTKDVEALIADGTISGGMIAKARSILKVLASGVPNVHGNLDVLTQILEGEIXEDLLENEKLLIEXDKKTX",
    "response#3": "MRCVIKYGGAAMVKESLKKSFCDDIGLLRSVGLRPVVVHGGGPEITRTLEKLGGKAEFIDGQRVTNASDVKVVEMVLTGSINTELVTLLNQEGVAHAVGVSGVTRINKDFLEMLLQQGYVPIISPVGLGEDGQSYNINADTVAAEVAIAIGAQKLIYLSDVPGILKGGELIGQLTTKDVEALIADGTISGGMIAKARSILKVLASGVPNVHLFTGNSAGILARLAAGEPVGTRFPPAPRELPAPRWLSRQWFLPRRDV"
  },
  {
    "reference": "MAAKWEKKDNNQGELTFEIAPDKIKEGLDKAFQRTKKNLAVPGFRKGRVPRQIFNQMYGEEALYQDALNIVLPEAYDAAIKEAGIEPVDQPQVDVESMDKDQPWVLKAVVTVKPDVKLGEYKGLSVTKQNTRVYQKDIDAEIEKQRQQQAELVLKEDEAAAKGDTVVIDFDGYVDGKQFDGGKADNYSLELGSNSFIPGFEDQLVGHKAGEDVDVKVTFPADYQAEDLRDKEATFKVTIHEVKEKQLPELDDEFAKDVDEDVDSLEELEAKTKDRLKEQKVTAAHDAIEDEAISEAVDNAEIAAVPEAMLKDDIDRQMDQYLANMQQQGIEPKMYFQLTGTTEDDLRKQFADGAEKRIKTNLVLEAVVEAEKIEPSEDEVAAEVKDLASQYGMEESAVRSALSDDMLKHDIAIKSAVDLIADSAKQDKKKDAEDDE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Involved in protein export; Acts as a chaperone by maintaining the newly synthesized protein in an open conformation2. The protein design should aim to mimic or enhance the function of natural proteins involved in protein transport, cell cycle, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "FDEEGGQKFAELTQAVAGTGRSLGVFLDNDLISAPVVGVEFANTGITGGAAVITGNFTIDTANDLAVQLRGGSLPFPVEVVENRTVGATLGQESIRRSLVAGFVGLVLVLVFMAVYYRLPGIVADISLMIYAVLTLAAFALVGVTLTLPGIAGFILSIGMAVDANVLIFERTREELRAGNTLYRSVEAGFFRAFSSILDSNVTTLIACAALFWFGSGLVKGFALTLAIGVMVSLFTAI",
    "response#2": "FDEEGGQKFAELTQAVAGTGRSLGVFLDNDLISAPVVGVEFANTGITGGAAVITGNFTIDTANDLAVQLRGGSLPFPVEVVENRTVGATLGQESIRRSLVAGFVGLVLVLVFMAVYYRLPGIVADISLMIYAVLTLAAFALVGVTLTLPGIAGFILSIGMAVDANVLIFERTREELRAGNTLYRSVEAGFFRAFSSILDSNVTTLIACAALFWFGSGLVKGFALTLAIGVMVSLFTAYSNKEIFLR",
    "response#3": "FDEEGGQKFAELTQAVAGTGRSLGVFLDNDLISAPVVGVEFANTGITGGAAVITGNFTIDTANDLAVQLRGGSLPFPVEVVENRTVGATLGQESIRRSLVAGFVGLVLVLVFMAVYYRLPGIVADISLMIYAVLTLAAFALVGVTLTLPGIAGFILSIGMAVDANVLIFERTREELRAGNTLYRSVEAGFFRAFSSILDSNVTTLIACAALFWFGSGLVKGFALTLAIGVMVSLFTALFSNYFFN"
  },
  {
    "reference": "MSDAMGVDVAPAGTLHRLASLWGCDVTSAEMAQQLDQHDKLASFRRPVAVMNGLTVNLHLLLTAFYHPTPLRHKILIEDRAFPSDKYAVRSQVRRHGYDPDNSVLLLKRRPGEDIFRMEDVLRTIRENASSIAVIMIGGVHYMTGQKFDMEAITKVGHEAGCVVGFDLAHAVGNVKLELHNWNVDFACWCTYKYLNSGPGCIAGAFVNNRHILSHLPLLHGWWGNKPETRFHMQDTIDHALGVNSLKLCNPPQMLVAMNRASLDIFDQVSMDQLVQKQRLLTGYLELLISLQLPEAEQITPADPAQRGCQLSFRFSNAHNIHAEMKRRGVECDIRHGEAGSPNVMRLAPVPLYNSFTDVFRLVTILRQAFHRTNSSASECTDSGSEISEDLGL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively.2. A protein that can perform kynureninase activity, pyridoxal phosphate binding in cytoplasm to support anthranilate metabolic process, quinolinate biosynthetic process, 'de novo' NAD biosynthetic process from tryptophan, tryptophan catabolic process, L-kynurenine catabolic process.3. The designed protein should contain one or more Aminotransferase class V domains that are essential for its function.4. The protein design should take into account the physiological conditions of the Cofactor biosynthesis, NAD(+) biosynthesis, quinolinate from L-kynurenine: step 2/3 pathway.5. pyridoxal 5'-phosphate is required for the proper folding and stability of the protein.6. The protein should have a low Km value for H2O + L-kynurenine = anthranilate + H(+) + L-alanine.7. The designed protein should have a high specificity for pyridoxal 5'-phosphate binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MGNSPLTQWQDMAEQCAKQSADIVGASPHEIVIMNTLTINLHLMMASFYKPSGKRHKIILEWKPFPSDHYAIESQVVWHGLDPKTSMIKIEPDANSVIPTESILRTIDEHADETALLMLPGIQYYSGQLFDMPRITAYAKQRGITVGWDLAHAAGNVELKLHEWDVDFACWCTYKYINAGPGAIGGVYVHERFGKL",
    "response#2": "MGNSPLTQWQDMAEQCAKQSADIVGASPHEIVIMNTLTINLHLMMASFYKPSGKRHKIILEWKPFPSDHYAIESQVVWHGLDPKTSMIKIEPDANSVIPTESILRTIDEHADETALLMLPGIQYYSGQLFDMPRITAYAKQRGITVGWDLAHAAGNVELKLHEWDVDFACWCTYKYINAGPGAIGGVYVHERFGKDPQPNKLVEKPWWDYHERFAEPLSKIVGAKPSEITVMNTLTVNLHLLMASFYRPNATRYKIICEEKAFPSDQYMIQSQIQFHGFTTSQALVEVKKRENEHQLRIEDIFATIDAHANDTALLLMGGVNYYTGQVFDMQAITKYAQERGIVVGWDLAHAAGNIEMQLHDWNVDFAAWCSYKYLFKNNKKYINEYIKKIDILSKKNN",
    "response#3": "MGNSPLTQWQDMAEQCAKQSADIVGASPHEIVIMNTLTINLHLMMASFYKPSGKRHKIILEWKPFPSDHYAIESQVVWHGLDPKTSMIKIEPDANSVIPTESILRTIDEHADETALLMLPGIQYYSGQLFDMPRITAYAKQRGITVGWDLAHAAGNVELKLHEWDVDFACWCTYKYINAGPGAIGGVYVHERFGKPAESQSPMTKEKPWWDYHERFAEPLSKIVGAKPSEITVMNTLTVNLHLLMASFYRPNATRYKIICEEKAFPSDQYMIQSQIQFHGFTTSQALVEVKKRENEHQLRIEDIFATIDAHANDTALLLMGGVNYYTGQVFDMQAITKYAQERGIVVGWDLAHAAGNIEMQLHDWNVDFAAWCSYKYRSKDPRPVHMNNMGHDISGALGMKLADELGTYLTNHTWKGYDKFIDHHTWLGNLPEIFGERFPQLLREKPWWDYHERFAEPLSKIVGAKPSEITVMNTLTVNLHLLMASFYRPNATRYKIICEEKAFPSDQYMIQSQIQFHGFTTSQALVEVKKRENEHQLRIEDIFATIDAHANDTALLLMGGVNYYTGQVFDMQAITKYAQERGIVVGWDLAHAAGNIEMQLHDWNVDFAAWCSYKYRSKDPRPVHMNNMGHDISGALGMKLADELGTYLTNHTWKGYDKFIDHHTWLGNLPKIFGERFPQLLRATNRLRHEDFMYE"
  },
  {
    "reference": "MAAESRCIVTPCSAICRTGKEVNFMQEIKPPQKSMFYYALTIILFMMIVNAFIMPSLKRASIEEVDYGTFMTMTENKQIEKVEFRSNDILFTDKDGKIYATGHMQDDGLVDRLYAAGAKFGSEIIPETSPMAAFLLSWVLPIAIFGGLGYLMSKRMMDRAGGGASSMMFGMGKSNAKVYVKSTEGIRFSDVAGEEEAKENLMEIVEYLHDPGKYKEIGATMPKGILLVGPPGTGKTMLAKAVAGESNVPFFSMSGSEFVEMFVGMGASKVRDLFRQAKEKAPCIVFIDEIDAIGKKRDGGVIGGNDEREQTLNQLLTEMDGFEENLGVVILAATNRPEALDPALTRPGRFDRRVPVELPDLQGRIDILKVHAKKIKIGTDVDFNKIARMAAGASGAELANIVNEAALRAVRDGRRFANQADMEESIEVVIAGYQKKNAILTDKEKLIVSYHEVGHALVAAMQTNSAPVQKITIIPRTSGALGYTMQVEEEGNHYLMSKEEIENKIATLTGGRAAEEVEFGSVTTGASNDIEQATRLARAMITRYGMSDEFDMVAMETVTNQYLGGDTSLACSAETQALIDRKVVELVRAQHEKALKILRDNKRKLDEIAEYLYFKETITGEEFMNILNRSPEPRRIGTQASPTTDDGMQAETDVQAEAHDNIDTQTAAEAQAKAHDDIDTQTAAEAEAEVRDGIDMRAEAGSQADAETRPEAEA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.2. The protein design should emphasize Basic and acidic residues compositional bias.3. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins4. The designed protein must possess ATP hydrolysis activity, ATP-dependent peptidase activity, ATP binding, zinc ion binding to support protein catabolic process under certain conditions.5. The presence of Zn(2+) is crucial for the protein to function optimally under specific conditions.6. The protein should be able to bind Zn(2+) ligand in a variety of conditions7. The presence of AAA+ ATPase domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MPGGVLLAGPPGTGKTLLARAVAGEAGVPFFSASASEFIEMIVGVGAGRVRELFAEARKVAPSIIFIDEIDTIGRARGGGGMGGHDEREQTLNQILTEMDGFSGSEGVVVIAATNRADILDKALTRPGRFDRTVTVSPPPHGSASRLAALAAGDPDSILPYDDDLLTARRVQHAQDPEQAFTVDDLADAVRSLLGPRPAPPRPAGPAARAREQD",
    "response#2": "MPGGVLLAGPPGTGKTLLARAVAGEAGVPFFSASASEFIEMIVGVGAGRVRELFAEARKVAPSIIFIDEIDTIGRARGGGGMGGHDEREQTLNQILTEMDGFSGSEGVVVIAATNRADILDKALTRPGRFDRTVTVSPPTRHGLGEGGDILRALGAATVLFPQQHEVDLKGIALATSGAVGSDLANMINEAAINAVKAHREYVCQQDLMYAIPHGVLLVGPPGSGKTLLAKAVAGEAKVPYFSISGSDFVEMFVGVGAARVRDLFEQARKSAPCIVFIDEIDAVGRKRGISLQGGNDEREQTLNQLLVEMDGFGSGQEVIILAATNRPDVLDAALLRPGRFDRQVVVDAPSRTGLGAGGDILSALGSATVLFPEDAAVAAGTGLSGADAAAEVA",
    "response#3": "MPGGVLLAGPPGTGKTLLARAVAGEAGVPFFSASASEFIEMIVGVGAGRVRELFAEARKVAPSIIFIDEIDTIGRARGGGGMGGHDEREQTLNQILTEMDGFSGSEGVVVIAATNRADILDKALTRPGRFDRTVTVSPPPRALLEPIDTHITLGAGKGDAAEALARWLLAHPPQGGGGFHVDLDRLARGTPGMSGADIENLVNEAALLASRRNRESVSMQDMESALGPVRSAIERQKDQALLDDAMSYGE"
  },
  {
    "reference": "MRSSVSRQTYSTKGAFSSSSASGGGGSQARTSFSSVTVSRNSGRGGGPRCGPSMGGFGSQSLYNLGGSKSISVSVAGGASAGRVLGGFGLGGGAYMGLGACRPMLGPVCPPGGIQQVTVNQSLLTPLHVEIDPEIQRVRTEEREQIKTLNNKFASFIDKVRFLEQQNKVLETKWALLQEQGQKSGVTRNNLEPLFEHFINNLRGKLDNLQSERGRLDSELRNVQDLAEDFKTKYEDEINKRTAAENEFVVLKKDVDAAYVGRMDLHGMVDHLMGEIDFLRHLYEEELSQVQTHVSDTSVILSMDNNRNLDLDSIIAEVKAQYEQIAQRSRAEAESWYQTKYEELQVTAGKHGDNLRDTKNEIAELTRTVQRLQGEADAVKKQCQQLQTAIADAEQHGELALKDAQKKLGDLDAALNQAKEDLARLLRDYQALMNVKLALDVEIATYRKLLESEESRMSGECPSAVSISVTGNSTTVCGGGAAGFGGGISLGGGGGASKGRFSTNAGYSTVKGGPVSGGTSILRKTTTVKTSSRRY",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that contributes to keratinization, intermediate filament organization by functioning in keratin filament with structural constituent of skin epidermis.2. The designed protein should contain one or more IF rod domains that are essential for its function.3. The designed protein's functionality will benefit from Polar residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "EKSTMQELNSRLASYLDKVQALEEANNDLENKIQDWYDKKGPAAIQKNYSPYYNTIDDLKDQIVDLTVGNNKTLLDIDNTRMTLDDFRIKFEMEQNLRQGVDADINGLRQVLDNLTMEKSDLEMQYETLQEELMALKKNHKEEMSQLTGQNSGDVNVEINVAPGKDLTKTLNDMRQEYEQLIAKNRKDIENQYETQITQIEHEVSSSGQEVQSSAKEVTQLRHGVQELEIELQSQLSKKAALEKSLEDTKNRYCGQLQMIQEQISNLEAQITDVRQEIECQNQEYSLLLSIKMRLEKEIETYHNLLEGGQEDS",
    "response#2": "EEKEQIKCLNNRFAAFIDKVRFLEQQNKLLETKLQFYQNRQCCESNLEPLFNGYIETLRREAECVEADSGRLSSELNSLQEVLEGYKKKYEEEVALRATAENEFVALKKDVDCAYLRKSDLEANVEALIQEIDFLRRLYEEEIRVLQAHISDTSVIVKMDNSRDLNMDNIVAEIKAQYDDIASRSRAEAESWYRSKCEEIKATVIRHGETLRRTKEEINELNRVIQRLTAEVENAKCQNSKLEAAVTQAEQQGEAALNDAKCKLAGLEEALQKAKQDMACLLKEYQEVMNSKLGLDIEIATYRRLLEGEEQR",
    "response#3": "QEREQIKALNNKFASFIDKVRFLEQQNQVLETKWNLLQQLDLNNCRKNLEPIYEGYISNLQKQLEMLSGDGVRLDSELRNMQDLVEDYKKRYEVEINRRTAAENEFVVLKKDVDAAYMNKVELQAKVDSLTDEIKFFKCLYEGEITQIQSHISDTSIVLSMDNNRDLDLDSIIAEVRAQYEEIALKSKAEAETLYQTKIQELQVTAGQHGDDLKLTKAEISELNRLIQRIRSEIGNVKKQCADLETAIADAEQRGDCALKDARAKLDELEGALHQAKEELARMLREYQELVSLKLALDMEIATYRKLLESEECRL"
  },
  {
    "reference": "MAPPGRRECAFPSRRFPGLLLAALVLLLSSFSDACEEPPTFEAMELIGKPKPYYDIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIGDPLNGQAILANGTYEFGYQIHFICNEGYYLIGNEILYCELKGSVAIWGGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSHAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLNGSNTIVCDSNSTWDPPVPKCLKGYPKPEEGILDSLDDWVIALIVIAIVVGVAVICAVLYGYLQRRKKKGNADGGAEYATYQTKSTTPAEQRG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The Extracellular, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.2. The protein must exhibit the following characteristics: Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells; May be involved in the fusion of the spermatozoa with the oocyte during fertilization; T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity .; Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue.3. The protein should have a specific function in complement activation, classical pathway, innate immune response, single fertilization and should be localized to cell surface, inner acrosomal membrane in order to carry out this function efficiently.4. Ensure the presence of a signal peptide within the protein.5. The designed protein should have at least Helical transmembrane domains to ensure proper localization.6. The protein contains novel Sushi domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "EMNEQCDCGTLKNCTHRKCCDPMSCRLKNKATCGSGECCSQDCTVKMNDVVCRKSVDECDFVEYCNGKDPYCVCHCDKGYNPPYCHCDKGYNPPYCHCDKGYNPPYCHCDKGYNPPYCHCDIGYNPPFCHCDKGYNPPYCHCDKGYNPPYCHCDKGYNPPYCHCDKGYNPPYCHCDKGYNPPYCHCDKGYNPPYCHCDKGYNPPYCHCDKGYNPPYCHCDKGYNPPYCHCDIGYNPPFCHCDKGYNPPYCHCDKGYNPPYCHCDKGYNPPYCHCDKGYNPPYCHYIPNPLHGEAILANGSYSFGNQLHFICNDGYYLIGKEILYCELKGSDAVWSGRPPICGPLTLELQIAKDERYGSYYNASDYPVVKLLREPIYVEVSIRHRTDPSLGLHLHQCWATPGMSPLLQPQWPMLVNGCPYTGDNYQTKLIPVQKASNLLFPSHYQRFSVSTFSFVDSVAKQALKGPVYLHCTASVCKPAGAPIL",
    "response#2": "PKLPLLPLLLLLLREAGAQDVRVRAAAEVRGHLGATVELPCHLLPPAPEIRVSQVTWLRLDAPEGNQNVAIFHPLYGPSFPSPKPGKERLSFVNAGQSTGAGQGTELEVRDATLALRGLTVEDEGNYTCEFATFPRGTSRGVPQNHAETQEVTLSLDPVPVARCVSTGGRPPARVSWLSPLDGEARESQVSGPLPGTVTVTSRFTLVPLGQADGVKVTCKVEHESFEEPVLLPVTLTWEPCPHIRDPLNGEAILANGSYEFGSELHFICNEGYYLIGKDILYCELKDTVAVWSGKPPLCPCTQDGHFSIAVSRNVTSPPLLWDSVHLAFRNDSECKPVMETHTFVLFRFPFSSCGTAKRVTGNQAVYENELVAARDVRTWSHGSITRDSIFRLRVSCIYAAEVRGHLGATVELPCHLLPPAPEIRVSQVTWLRLDAPEGNQNVAIFHPLYGPSFPSPKPGKERLSFVNAGQSTGAGQGTELEVRDATLALRGLTVEDEGNYTCEFATFPRGTSRGVTWLRVNHAETQEVTLSLDPVPVARCVSTGGRPPARVSWLSPLDGEARESQVSGPLPGTVTVTSRFTLVPLGQADGVKVTCKVEHESFEEERCTQDGHFSIAVSRNVTSPPLLWDSVHLAFRNDSECKPVMETHTFVLFRFPFSSCGTAKRVTGNQAVYENELVAARDVRTWSHGSITRDSIFRLRVSCIYSVSSSALPVNIQVFTLPPPLPETHPGPLTLELQIAKDERYGSYYNASDYPVVKLLREPIYVEVSIRHRTDPSLGLHLHQCWATPGMSPLLQPQWPMLVNGCPYTGDNYQTKLIPVQKASNLLFPSHYQRFSVSTFSFVDSVAKQALKGPVYLHCTASVCKPAGAPICVTTCPA",
    "response#3": "LNGRLCKLGTAYCYNGQCQTTDNQCAKIFGKGAQGAPFACFKEVNSLHERSENCGFKNSQPLPCERKDVLCGKLACVQPHKNANKSDAQSTVYSYIQDHVCVSIATGSSMRSDGTDNAYVADGTMCGPEMYCVNKTCRKNGQYCESGEAFCFEGRCQTADKQCMSMLGKYVRGASFACYEEFNSRGDRFGNCIHNFCAFRNSLCGKLICTWPFKKLVLKANLSVAYAQIRDDLCVAMCHCDKGYNPPYCRNGQYCDSGQAFCYSGLCMTSNNQCMNLLGKYVRGASFACYEEFNSRNDRFGNCIRKFCSFENSLCGKLVCTWPFKRLLMKDNMSAAYGQIRDDLCISLYKGGRPLKTTLSTYSDMSERDETFVKDGTICGPDMFCLETQCKENGRLCKLGTAYCYNGQCQTTDNQCAKIFGKGAQGAPFACFKEVNSLHERSENCGFKNSQPLPCERKDVLCGKLACVQPHKNANKSDAQSTVYSYIQDHVCVSIATGSSMRCHCDKGYNPPYCSLNGRLCKLGTAYCYNGQCQTTDNQCAKIFGKGAQGAPFACFKEVNSLHERSENCGFKNSQPLPCERKDVLCGKLACVQPHKNANKSDAQSTVYSYIQDHVCVSIATGSSMRSDGTDNAYVADGTMCGPEMYCVNKTCRKCHCDKGYNPPYCHCDKGYNPPYCHCDIGYNPPFCSIRNGQYCDSGQAFCYSGLCMTSNNQCMNLLGKYVRGASFACYEEFNSRNDRFGNCIRKFCSFENSLCGKLVCTWPFKRLLMKDNMSAAYGQIRDDLCISLYKGGRPLKTTLSTYSDMSERDETFVKDGTICGPDMFCLETQCKE"
  },
  {
    "reference": "MTPKDMMVADANAEAMGIPRTSLMENAGRGLSQVISQHKEPCKVTIYAGNGGNGGDGFVAARYLLNMGFEVEVYLLSDPALIKSREASLNWEVLQSINKGTSPLKVEVLKDSAYLHPTSAEVVVDALLGTGVRGNLREPVSTAVDVINQARGLKVAVDMPTGVDPGSGMVDDKAVRADITVTFHKVKVGLNIASVEYVGSIEVCDIGIPLEAELFTGPGDLLRRGRRDEKSHKGQNGKVLVIGGSKEYSGAPALAALAALGGGADISVVACPQELSSIIRSYSPDLIVHRLSNEFLNPKDTEELIKLSEKFDAVVLGCGIGREEETALAVNDLVVEIGKPMVIDADALKMLGPGVIPRRIHDTVITPHTGEFHEFSGRTPPQDMQDKMEMVKEVALESESTVLLKGAVDIIARTDRIKLNRTGNPGMSVGGTGDCLAGLTGALLAQGHGGFEAAFLAAYINGMAGDLAAEEYGYNFSATDLLSYIPRIF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be able to perform ATP binding under specific conditions.2. The protein contains novel YjeF N-terminal, Rhodanese domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "PDDHKYTRGVVGVRAGSETYPGAALLCVAGASSGLVGMVRYVGSAADRVRVAHPEVVGDGRVQAWVVGPGGGDEAAEQLAAVVGSGVPVLVDADGLAHVRPGQAAVLTPHAGELARMLDTAREDVEARPLAHARLAAERYDAVLLLKGRHTLVVAPDGRTRVTTTGLPWLATAGAGDVLSGLIGALLAAGLDPWDAASVGSWLHGAAATLASGGGPLVAGDVAAALF",
    "response#2": "LDTFNLPALASRPKAAHKGLYGRVLVIGGDHGFGGAALLSAESALRSGAGMVTLATRSEHVPAALVRMPEVMSAAIHSANQLLALIEPAAVLVVGPGLGQASWGRCLLSAAADAGRPQVWDADALNQLAGGIVSLPVNSVITPHPGEAARLLGISTQEVQADRPSAARALARKFNTVCVLKGSGSLIVDPDGRLALCDRGHPAMATAGLGDVLAGLIGALLAQHMTPFDAACLGVWLHARAGEQAGASGRGLAASDTIPAIRQLLEEA",
    "response#3": "GSHKHARGHLAVVSGNIISTGAARLAGRAGLRAGAGLVTMLSPPDAIAVNASHLTAVMLKEVADAEQLRAAIESASVVILGPAAGINTSTRENVECLLSSNCRAVLDADALTVFSDDPGAFFKQLRPDDLITPHIGEFNRLFGDLLETEPNKVEATRKAAQLAGCVVLLKGPDTVIARPDGPAIVNTHATRWLATAGSGDVLAGIAGGLIAQGVDTFTAAAMASWLHGEAGRRIGAGLIAEDLEAELS"
  },
  {
    "reference": "MITSQQRALVKATVPLLETGGEALTRHFYAMMLSEYPEVRPLFNQAHQASGDQQRALANGILMYAKHLDHLEALGPLAGKIVNKHVSLQILPEHYPIVGTCLLRAIREVLGADIATDEVLEAWGVAYGILADLLINAEEGMYAANAEAPGGWRGSRTFVLVRKVAESEEIVSFHFAPADGKPVLAYQPGQYITLLLDLDGEQARRNYSLSASSNGIEYRISVKREPEGRVSNFLHDKLQEGDRIEMFAPAGDFVLRESSKPLALITAGVGITPTLSMLEAAQASGRAITFIHCARNGAVHAFRDWIEEHVSRNPALKRFFCYSEPRETDQGDATGFLTRERLGEWLPQDRDLDVYFLGPKPFMAQVKKHLGDLGVPASQCHYEFFGPASALEA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The presence of heme b is crucial for the protein to function optimally under specific conditions.2. The NADP(+), heme b, FAD binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein is the optimization of the Charge relay system to achieve high catalytic efficiency for NADPH + 2 nitric oxide + 2 O2 = H(+) + NADP(+) + 2 nitrate.4. The protein contains novel FAD-binding FR-type, Globin family profile domains that confer a unique function or activity.5. The designed protein must possess oxygen carrier activity, nitric oxide dioxygenase activity, FAD binding, oxygen binding to support response to nitrosative stress, response to toxic substance under certain conditions.6. The protein must exhibit the following characteristics: Therefore, plays a central role in the inducible response to nitrosative stress.; Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. The designed protein sequence is ",
    "response#1": "MLSTEHKAIISATVPILEQGGETLTRHFYQSLFRDYPGVKPLFNQSNQQDGNQQRALANAVLMYAKNIEKLENLGPLVSTIINKHVSLQIQPEHYPIVGASLLKAIREVLGAEVATDAVLEAWGAAYGQLADILIGTPDEAAYRDEGLREFRLVNKQPQSEVITSFEFVPVDGKPVASYRPGQYITIYIHDESFANQEIRQYSLTTAPNGQSYRIAVKREDGGLVSQHIHQKLNEGDIVHLAPPCGDFFIKPGADTPVVLISGGVGITPLFSMASTIATEHPARDVTLLYGATSATTHAMADRLAELATTHDNVRVFTAYDAPGAGDHDGYHKEGFVTAEWLRAHVPEDADFYVCGPVPFMEAIHRALAAVGVPAAHRHYEFFGPAAALDAP",
    "response#2": "MLSENTIKIVKSTAPVLAERGVEITSYFYKTMFEAHPELLNIFNHANQKQGRQQTALANTVYAAATYIDQLEVLLPAVKQIAHKHRSLNVKKEHYPIVGEFLLKAIKDVLGDAATPEVLQAWGEAYQVIAQVFVGYRDFRVIQKVKESDVITSFYLKPADSGLLPDFKAGQYITVKVNVPSIPYTCQRQYSLSCKPNKEFFRISVKREDSIGDSPDGVVSTYLHESVNEGNIIELSAPSGDFVLEHSDTVPVVLLSGGVGLTPMISMLNTLVASQKLISAGVGLTPMLAMLESLSEHRAPLTWVHATENGQQHAFKQHAKALVSKLEQGQTAIWYHQPSTEDRLGDDFHFTGLVNLDEIRDKLQAEHAQVYLCGPIGFMQYAYLQQLGLAPEQLHYECFGPHKVL",
    "response#3": "MLSQNTIKVIKSTVPVLEVHGEAITRHFYETMFEHHTELLNIFNHANQKQGRQQAALANMVYTAAQHIDNLGSILPAVRQVAHKHRSLGIVPEQYAIVGTYLLQAIKDVLGDAATDEIITAWSEAYNVIADAFIGREADIYASQRAEGGWKGFRSFKVAKKVKESECITSFYFVPEDGLPIASYEPGHYISIRLHPEGHAFTQIRQYSLSDAPGKPYYRISVKRECAVMERPDGVISTYLHEHITEGSVVELSAPAGDFVLDAESRPVVLISGGVGLTPLTSMLETVADEHPARPITFIQAARNGRVHAMRDAVAEIAERHPNLRVEICYSAPTEGDKQDRFHRTGLLDLEWLSETVGDDNAQVYLCGPIPFMRAIAGQLRAWGVAEDRIHYEVFGPHADLA"
  },
  {
    "reference": "MAPVSDVEVSDRGAAGRTGGVLSLDDCRCPVCLEIFMEPVTLPCTHTFCKDCFLESVDKATLCCPLCRKRVSTWARLNNRNNTMVNQQLWTRIQSTFPLQCQRRLSGQDHANDDDLGATDTSLCYPRVSQPGEVRQEYEDQVTKLTEEKRVLDEEERRASEEYIQRLLAEEEELLQEDRRRREEDERLARQLSTQLNSAIVSQEVVTPAKKKEVSGQIEKFLCPRPSKSSSDCSFTANKENILVSPVKLQAECSPPKLDYYGDTSDPPPPSGEEEEEERDLPESHRSHLTGDGGPSLAKRKSSELETTEEEKVTKRACRSLPSSSSSYSSLEGGGSVLQGIVDWESELQSRRQQEEEDRRLALLLQKELDQEEKQRATDRRKGSSDAYLLRQNRRGKEDASSSQTPRRSSTKTTKTSSASSSRGSKQTTLTDMFSCLNS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination.2. The designed protein should have a specific function within the ubiquitin ligase complex. The designed protein sequence is ",
    "response#1": "IEVDTAHYMLVKAELLQEMTELEEKYDTRCEVWGDSNKIHILFEPKDKEFDLSAHAYSSFIDAYQYVSSQLMREVLSLKPLGKERKHLHQNKFADDFRENHPHVHFVLNQESMTLTGLPNHLAKAKQL",
    "response#2": "KLQRILEDLSEPPFDPGQYINLYTIIYDMCLQQPPNDYSQELYNKYRGVVDHYNKETVLPSMRERHGEYMLRELVKRWANHKILVRWLSRFCFYLDRFYVARRGLPTLNDVGFTSFHDLVYQEIQSEAKDVLLALIHKEREGEQIDRTLVKNVIDVYCGNGVGQMVIYEEDFESFLLQDTASYYSRKASRWSQEDSCPDYMLKAEECLKLEKERVTNYLHSTTEPKLVEKVQNELLVVVAKQLIENEHSGCLALLRDDKMGDLSRMYRLYRLIPQGLEPIADLFKQHVTAEGNALIKQAADAATNQDASASQVLVRKEIELHDKYMVYVDECFQKHSLFHKLLKEAFEVFCNKTVAGASSAEILATYCDNILKTRGGSEKLSDEATEITLEKVVNLLVYISDKDLFAEFYRKKQARRLLFDRSS",
    "response#3": "GMRNHPLDLPVQFTASACALNLTRQELARGMPVRLLAEITDLLFKATKNFPYYQQLQKNCLLSLTSSRILMDVPFDRFDAAKLALRWVCRRESPKLRTMAVSITSILALKLSPEEMGQLQEELIMAIKELLTIIRQKLAENLDDVTFLFTLKALWNLTDECPLACKYFMENEGLATVIRVLETFSISVIQSKVLGLLNNVAEVRELSSKLVTEDVIERIISLLHSSNLEVSFLAAGVLAHLTCDRQHWLSRDLQRTDLLRYLHLAIQNWPSSRCDMSVLVTYRSFKAFSPLLVNFSQPEVQRWALWAIHHVCSKNPC"
  },
  {
    "reference": "MVLAAAMSQDADPSGPEQPDRVACSVPGARASPAPSGPRGMQQPPPPPQPPPPPQAGLPQIIQNAAKLLDKNPFSVSNPNPLLPSPASLQLAQLQAQLTLHRLKLAQTAVTNNTAAATVLNQVLSKVAMSQPLFNQLRHPSVITGPHGHAGVPQHAAAIPSTRFPSNAIAFSPPSQTRGPGPSMNLPNQPPSAMVMHPFTGVMPQTPGQPAVILGIGKTGPAPATAGFYEYGKASSGQTYGPETDGQPGFLPSSASTSGSVTYEGHYSHTGQDGQAAFSKDFYGPNSQGSHVASGFPAEQAGGLKSEVGPLLQGTNSQWESPHGFSGQSKPDLTAGPMWPPPHNQPYELYDPEEPTSDRTPPSFGGRLNNSKQGFIGAGRRAKEDQALLSVRPLQAHELNDFHGVAPLHLPHICSICDKKVFDLKDWELHVKGKLHAQKCLVFSENAGIRCILGSAEGTLCASPNSTAVYNPAGNEDYASNLGTSYVPIPARSFTQSSPTFPLASVGTTFAQRKGAGRVVHICNLPEGSCTENDVINLGLPFGKVTNYILMKSTNQAFLEMAYTEAAQAMVQYYQEKSAVINGEKLLIRMSKRYKELQLKKPGKAVAAIIQDIHSQRERDMFREADRYGPERPRSRSPVSRSLSPRSHTPSFTSCSSSHSPPGPSRADWGNGRDSWEHSPYARREEERDPAPWRDNGDDKRDRMDPWAHDRKHHPRQLDKAELDERPEGGRPHREKYPRSGSPNLPHSVSSYKSREDGYYRKEPKAKS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.2. The designed protein must have at least one RRM domain.3. The designed protein should have mRNA binding, zinc ion binding, RNA binding, splicing factor binding within nucleus to regulate mRNA processing, heart formation, regulation of mRNA splicing, via spliceosome, RNA splicing, negative regulation of mRNA splicing, via spliceosome.4. For general function, the protein need meet that Together with RBM24, promotes the expression of short isoforms of PDLIM5/ENH in cardiomyocytes .; RBM20- mediated exon skipping of TTN provides substrates for the formation of circular RNA (circRNAs) from the TTN transcripts; RBM20-mediated exon skipping is hormone-dependent and is essential for TTN isoform transition in both cardiac and skeletal muscles; Acts as a repressor of mRNA splicing: specifically binds the 5'UCUU-3' motif that is predominantly found within intronic sequences of pre-mRNAs, leading to the exclusion of specific exons in target transcripts The designed protein sequence is ",
    "response#1": "MPGFPYPAATAAAAYRGAHLRGRGRTVYNTFRAAAPPPPIPAYGGVVYQDGFYGADIYGGYAAYRYAQPTPATAAAYSDSYGRVYAADPEASLDPGPPPARFFLIKSNNHENVSLAKAKGVWSTLPVNEKKLNLAFRSARSVILIFSVRESGKFQGFARLSSESHHGGSPIHWVLPAGMSAKMLGGVFKIDWICRRELPFTKSAHLTNPWNEHKPVKIGRDGQEIELECGTQLCLLYEAAPVQATPRLRGLPFGCTKEEIVQFFSGLEIVPNGITLPVDPEGKITGEAFVQFASQELAEKALGKHKERIGHRYYRLDPPTSLKGTPGTRRTKLFVGMLNKQQCEDDVRRLFESFGSIEECTILRGPDGNSKGCAFVKYSTHAEAQAAISALHGSQTMPGASSSLVVLNPDNKEPDAIKLFIGQIPRNLDEKDLKPIFEQFGKIYELTVIKDKFTGMHKGCAFLTYCARESALKAQSALHEQKTLPGMNRPIQVKPADEYVLGKDSPNSPAELVAQITVLQLHNLITGDEIMDVQEYEDIYESVKTQFSNYGPLIDIKIPRSIGTRNSGLGTGKVFVRYSDIRSAEVAMEEMKGCKFNDRTIVILNPSSTFAQHLRDVEKHKKSINGLINKVNISNISIIIQELLQENIVRGRGLLSRSVLQAQSASPIFTHVYAALVAIINSKFPQIGELILKRLILNFRKGYRRNDKQLCLTASKFVAHLINQNVAHEVLCLEMLTLLLERPTDDSVEVAIGFLKECGLKLTQVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAVRKD",
    "response#2": "LRRTIYLTLNSCLDYEECAQKLMKMQLKTCQQNEFCQILLDCCAEQRTYEKFYGLLTHRICKMNKSFIEPFKEIFKDICQTTHCLDTNRLRNISKFFAHLLFTDAIRLRGLPFGCTKEEIVQFFSGLEIVPNGITLPVDPEGKITGEAFVQFASQELAEKALGKHKERIGHRYLALRRTIYLTLNSCLDYEECAQKLMKMQLKTCQQNEFCQILLDCCAEQRTYEKFYGLLTHRICKMNKSFIEPFKEIFKDICQTTHCLDTNRLRNISKFFAHLLFTDAISWDVLDCIKLSFRRTIYLAIQSSLDFEECAHKLLKMEFPESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCMLKKEYMESFEGIFKEQYDTIHRLETNKLRNVAKMFAHLLYTDSLPWSVLECIKEARFFLIKSNNHENVSLAKAKGVWSTLPVNEKKLNLAFRSARSVILIFSVRESGKFQGFARLSSESHHGGSPIHWVLPAGMSAKMLGGVFKIDWICRRELPFTKSAHLTNPWNEHKPVKIGRDGQEIELECGTQLCLLLPGLPEGLSVLALSFRRTIYLAIQSSLDFEECAHKLLKMEFPESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCMLKKEYMESFEGIFKEQYDTIHRLETNKLRNVAKMFAHLLYTDSLSARFFLIKSNNHENVSLAKAKGVWSTLPVNEKKLNLAFRSARSVILIFSVRESGKFQGFARLSSESHHGGSPIHWVLPAGMSAKMLGGVFKIDWICRRELPFTKSAHLTNPWNEHKPVKIGRDGQEIELECGTQLCLL",
    "response#3": "MAIKDDLVVAEVEINDVPLTCRNLLTRGQTQDEISRMSGAAVSTRGRYMTAEEKAKIGPGDRPLYLHVQGQTRELVDRAVNRIKEIKDDLVVAEVEINDVPLTCRNLLTRGQTQDEISRMSGAAVSTRGRYMTAEEKAKIGPGDRPLYLHVQGQTRELVDRAVNRIKEINTIFVQGLGENVTIESVADYFKQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWFDGKEFSGNPIKVSFATATFNVKEKVEGPGCSYLQHIQLETGAKVFLRGKGSGCIEPASGREAFEPMYIYISHPKPEGLAAAKKLCENLLPQRFFLIKSNNHENVSLAKAKGVWSTLPVNEKKLNLAFRSARSVILIFSVRESGKFQGFARLSSESHHGGSPIHWVLPAGMSAKMLGGVFKIDWICRRELPFTKSAHLTNPWNEHKPVKIGRDGQEIELECGTQLCLLLHYTNQL"
  },
  {
    "reference": "MYTLKQDRPHPVPPPPPLTMDHLQHGYSTNTPVTLPGIPEAARSVSTVYEGRIYSLDVVQQPIRARMCGFGDKDRRPLTPPPCIRLIVRDATTQKEIDINEIDTSFYVLTVDLWNAEGTSEVNLVRHSATSPSISTATSSAYPPPANIMPFPQYAQTHGYPQTAYPPYYGGQPGYNPHAGYQYPQGGPDPYYPGLPAASTYYPPGTPTQALGPQSALGPHISSASTGMFTRNLIGSLSASAFRLTDPDDKIGVWFVLQDLSVRTEGSFRLKMNFVNVGTQSQAQDQSPGGTPSSPVINVGSAPVLASAFSEVFQVYSAKKFPGVIESTPLSKCFAFQGIKIPIRKDGVKGPRGGQSHGSKGQDGEGEDWENEG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Component of the velvet transcription factor complex that controls sexual/asexual developmental ratio in response to light, promoting sexual development in the darkness while stimulating asexual sporulation under illumination ; The velvet complex acts as a global regulator for secondary metabolite gene expression ; The complex binds DNA through the DNA-binding domain of RYP2 that recognizes an 11-nucleotide consensus sequence 5'-CTGGCCGCGGC-3' consisting of two motifs in the promoters of key developmental regulatory genes . The designed protein sequence is ",
    "response#1": "CGGCCGCGG",
    "response#2": "GSGWKYSLDVKQQPVRARMCGFGDKDRRPITPPPCVRLVIINTETGKEVDYNSLDHAMFVLSVDLWNHDGTKEVNLVRSSTGTGAMASSSTYTYSSLEPGTPSYQQQSLPPSRESGYGQSQGLNYGQDYPPPVQQSYGQAPSYPPSSSYGPPQQYYPRHSGYSAEPSAPPPGAPFRNGYGQDQNALTRMAVVGGQPQGMFTRNLIGSLAASAFRLEDTEGQSGIWFVLQDLSVRTEGTFRLRFSFVNVGRPGGQGTNVNQGRAPILSSCYSESFHVYSAKKFPGVCESTPLSKKFANQGIKIPIC",
    "response#3": "CGGCCGCGGC"
  },
  {
    "reference": "MHEKWVVASSNAGKIAELEALLDGLAITPVSQRDFDVDDAVEDGIAFLDNALIKARHAASQTGLPAIADDSGLAVDWLDGAPGIFSARYAGTHGDDEANNAKLLTELDGVAAADRGAAFHCCLVSVRSPADPAPIVAHGVWRGRILTRGRGTGGFGYDPLFWVDSEQASAAELAPARKNTISHRGRAMSRLVEALRERRDR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must possess XTP diphosphatase activity, dITP diphosphatase activity to support nucleotide metabolic process under certain conditions.2. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP The designed protein sequence is ",
    "response#1": "MPIGVPKVPFRSPGPDE",
    "response#2": "MLKVTFVTSNPHKLHEVQAIL",
    "response#3": "EIQRLP"
  },
  {
    "reference": "MIAIGMEGSANKVAIGIILHPTDGSTPQVLANVRHTYNAPPGEGFLPKDTARHHRAWVVKMVKAALQEASLSPQDVDCICFTKGPGMGAPLQSVAIAARMMSLLWDKPLVAVNHCVGHIEMGRLITGAENPVVLYVSGGNTQVIAYSDKRYRIFGETLDIAVGNCLDRFARTLHIPNDPFPGYNIEQLAKKGSRLVDLPYVVKGMDCSFSGILASIDALAVSLGLNKQEQTDQSPADQPTRADLCFSLQETVYAMLVEITERAMAHVGSKQVLIVGGVGSNERLQEMMGIMARDRGGSVFATDERYCIDNGIMIAQAGMMAYKTGFQTPLKESTCTQRFRTDETLEMVVTPPAGTMGASPNPNGPKSGLPASTPRSSDQSASEQSKRKQAALLDKKALELEDEFKAKHFGVMGVLAWILLLHVAGIYLFTKGFLLTRMVLENKSSCDVLPFEGILAQPHTNLGSKQQKGCWHEKTFDKAVVIIIDALRYDFTVPFASKGESDTVHLFHNNIPVFYETAVQNPANAFLLPFIADPPTTTLNRLKGLTTGTLPTFLDAGSNFAGTAIDEDNLVAQLHSAGKNLVHLGDDTWQALFPGYFDPNLTHAYDSFNVWDLHTVDNGVNEHLFPLLRPENSKKWDVIFGHYLGVDHAGHRYGPNHAAMASKLQEMDRVIRDIIAALDDKTLLVVMGDHGMDVKGDHGGESDDEVEAALWMYSKRGIFGRTSKDTLLPPQLARDRFVPQIDLVPTLSLLLGMPIPFNNLGSPIEEVF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must have at least one Gcp-like domain.2. For general function, the protein need meet that The complex is required for efficient recruitment of transcriptional coactivators.; The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation3. A protein that is capable of catalyzing reactions within the Glycolipid biosynthesis pathway with high efficiency and specificity.4. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.5. A protein with N(6)-L-threonylcarbamoyladenine synthase activity, metal ion binding functions is required to enable GPI anchor biosynthetic process. The designed protein sequence is ",
    "response#1": "VKGFGGSLSDAAAMNILGLSQPAQDNLLRSYFSESGIEYNLVRLPMACSDFSTRPYSYDDVPHDYELTHFRLAEEDVKMKVGGRRPSAMSKRPLLLYASPWTSPAWMKSNGDVRGKGTLKGHAGDKYHKTWANYFIKFLDEYAKHNVTFWAVTAQNEPLAALLTPPEFPTIAFTAVQQRDFVVRDLGPALARSPHRTRLIILDDQRIHLPHWAKAVLGNATAASYVAGVGVHWYLDSIVPASCSLEATHKLFPHHFLLYTEACSGFLNLRFSVTLGCWERGDHYSHSILY",
    "response#2": "MRVLGIEGTAWAASAACYDDTGDSVFIESDPYQPDSGGIHPREAAEHMGEALPNVIDSVLTHAAETVDDAADTATNDTDSPNRYGIDCVAFSQGPGLGPCLRTVGTAARAAAQALNVPLVGVNHMVAHLEIGRHQSGFDSPVCLNASGANAHLLGYHNGRYQVLGETMDTGVGNALDKFTRHIGWEHPGGPKVEAAATDGELIDLPYVVKGMDFSFSGIMSAAKDAADEGEAIEDICFSLQEHIFGMLTEVAERALSLTGTDELVLGGGVGQNARLQSMLETMCEERGAAFDVPDNRFLRDNAGMIAVLGAKQFAAGQTITIEDSTVDPNFRPDEVAVAWSG",
    "response#3": "MRVLGIEGTAWAASAACYDDTGDSVFIESDPYQPDSGGIHPREAAEHMGEALPNVIDSVLTHAAETVDDAADTATNDTDSPNRYGIDCVAFSQGPGLGPCLRTVGTAARAAAQALNVPLVGVNHMVAHLEIGRHQSGFDSPVCLNASGANAHLLGYHNGRYQVLGETMDTGVGNALDKFTRHIGWEHPGGPKVEAAATDGELIDLPYVVKGMDFSFSGIMSAAKDAADEGEAIEDICFSLQEHIFGMLTEVAERALSLTGTDELVLGGGVGQNARLQSMLETMCEERGAAFDVPDNRFLRDNAGMIAVLGAKQFAAGQTITIEDSTVDPNFRPDEVAVAWLG"
  },
  {
    "reference": "VRDGYIMIKDTNCKFSCNIFKKWEYCSPLCQSKGAETGYCYNFGCWCLDLPDDVPVYGDRGVICRTR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be able to perform sodium channel inhibitor activity under specific conditions.2. For general function, the protein need meet that Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. The designed protein sequence is ",
    "response#1": "KEGYPMGRDGCKISCVINNNFCKVECQAKWRQSDGYCYFWGLSCYCTIYQKTPRFGILAPINVEDNVTVTQCR",
    "response#2": "KEGYPMGRDGCKISCVINNNFCKVECQAKWRQSDGYCYFWGLSCYCTIYQKTPRFGILAPINVEDNVTVTQCR",
    "response#3": "KEGYPMGRDGCKISCVINNNFCKVECQAKWRQSDGYCYFWGLSCYCTIYQKTPRFGILAPINVEDNVTVTQCR"
  },
  {
    "reference": "MVKVIIASNNENKIREIKDIFLGYPVNVISMKEAGINIEIVEDGSTFMENAYKKAKQVHDITGDIVLADDSGLMVKGLNGAPGIYSARFCGEHGNDKKNNEKLLKMLEGKSEEDRKAKFVCAMVLIFNDSKVIKVKGEVDGVIAHEELGKNNFGYDPLFYVPKYNMTFAQMPAKLKNSISHRAQALEALKEKFDTFMKKV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design must be able to perform nucleotide binding, ITP diphosphatase activity in order to contribute to nucleotide metabolic process.2. The protein should have a low Km value for dITP + H2O = dIMP + diphosphate + H(+).3. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..4. The substrate, Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MGTFILSGIIIAISLIILAAI",
    "response#2": "MEVRFVGLIGTWNSGKDTVANILQKEFPF",
    "response#3": "MTPIILASGNAGKVREFNRLGL"
  },
  {
    "reference": "MQKLVIHGGSKLHGEISISGAKNAALPVLCASLLTAEPFAIQNIPHLRDVTTMLALLEQIGVRILTNEPGTTELSAANITNPTASYDMVKTMRAAILVLGPLLARTGQAHISLPGGCAIGMRPVDQHIKGLQAMGAEINIEQGYILAQVGRLSGARIAMDVVTVTGTENLMMAATLASGTTILENAAREPEVIDLANCLIGMGAKIEGAGNDIIIIEGVDRLHGGSHVVMPDRIETGTFLTAVAACGGDVTLIGTRADTLDVVLGKLAEAGADIDIGGDWIRLCMQQRPQPVSLRTAPYPAFPTDMQAQFMALNSIAGGTSIMTETIFENRFMHVQELTRLNADIQVEGNTAIVHGIPQLDGASVMATDLRASACLIIAGLIAQGETIVDRIYHLDRGYEQIEQKLAQVGAHIKRIN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity within cytoplasm to regulate cell wall organization, cell division.2. For general function, the protein need meet that Adds enolpyruvyl to UDP-N- acetylglucosamine.3. The designed protein should have a binding site that is specific to the target UDP-N-acetyl-alpha-D-glucosamine ligand.4. A protein should be able to manipulate the activity of enzymes in the Cell wall biogenesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "VDGGHPLSGEVHVRGAKNFVSKAMVAALLTRETSVLRNVPQIRDVEVVSDLLRLHGVEVDYQPEEGTVTITPGEIHLPENPLEVNALAGSSRIPILFAGPLLHALGEAFIPDLGGCHIGARPVDFHLRVLEEFGAKRVQEAAGMHLVAPKGLTAKPVRLPYPSVGATEQTLLTAVMADGITVLSNAAVEPEIEDLINVLQKMGAVISLDTDRTIRIEGVKELHGFHHTALPDRIEAGSWASAALATHGDIFVRGAQQQPMVSFLNTFRKAGGKFDVRDDGIRFWHPGGDLNAILVETDVHPGFMTDWQQPLVVALTQSNGLSIVHETVYENRFGFTQALREMGANIQTYRECLGGHECRFGQRNFYHSAVIQGPTPLRGADIHVPDLRGGFSHLIAALAAEGESRVSGVDVIDRGYENFVGKLMT",
    "response#2": "VDGGHPLSGEVHVRGAKNFVSKAMVAALLTRETSVLRNVPQIRDVEVVSDLLRLHGVEVDYQPEEGTVTITPGEIHLPENPLEVNALAGSSRIPILFAGPLLHALGEAFIPDLGGCHIGARPVDFHLRVLEEFGAKRVQEAAGMHLVAPKGLTAKPVRLPYPSVGATEQTLLTAVMADGITVLSNAAVEPEIEDLINVLQKMGAVISLDTDRTIRIEGVKELHGFHHTALPDRIEAGSWASAALATHGDIFVRGAQQQPMVSFLNTFRKAGGKFDVRDDGIRFWHPGGDLNAILVETDVHPGFMTDWQQPLVVALTQSNGLSIVHETVYENRFGFTQALREMGANIQTYRECLGGHECRFGQRNFYHSAVIQGPTPLRGADIHVPDLRGGFSHLIAALAAEGESRVSGVDVIDRGYENFVGKLLG",
    "response#3": "VDGGHPLSGEVHVRGAKNFVSKAMVAALLTRETSVLRNVPQIRDVEVVSDLLRLHGVEVDYQPEEGTVTITPGEIHLPENPLEVNALAGSSRIPILFAGPLLHALGEAFIPDLGGCHIGARPVDFHLRVLEEFGAKRVQEAAGMHLVAPKGLTAKPVRLPYPSVGATEQTLLTAVMADGITVLSNAAVEPEIEDLINVLQKMGAVISLDTDRTIRIEGVKELHGFHHTALPDRIEAGSWASAALATHGDIFVRGAQQQPMVSFLNTFRKAGGKFDVRDDGIRFWHPGGDLNAILVETDVHPGFMTDWQQPLVVALTQSNGLSIVHETVYENRFGFTQALREMGANIQTYRECLGGHECRFGQRNFYHSAVIQGPTPLRGADIHVPDLRGGFSHLIAALAAEGESRVSGVDVIDRGYENFVGKLKAF"
  },
  {
    "reference": "MMIKKNASSEDFFILLGFSNWPQLEVVLFVVILIFYLMTLTGNLFIIILSYVDSHLHTPMYFFLSNLSFLDLCHTTSSIPQLLVNLRGPEKTISYAGCMVQLYFVLALGIAECVLLVVMSYDRYVAVCRPLHYTVLMHPRFCHLLAAASWVIGFTISALHSSFTFWVPLCGHRLVDHFFCEVPALLRLSCVDTHANELTLMVMSSIFVLIPLILILTAYGAIARAVLSMQSTTGLQKVFRTCGAHLMVVSLFFIPVMCMYLQPPSENSPDQGKFIALFYTVVTPSLNPLIYTLRNKHVKGAAKRLLGWEWGK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should contain Cytoplasmic topological domains.2. For general function, the protein need meet that Odorant receptor.3. The protein can be expressed in plasma membrane and perform G protein-coupled receptor activity without triggering undesirable cellular responses.4. The protein should contain a Helical; Name=3, Helical; Name=5, Helical; Name=7, Helical; Name=6, Helical; Name=1, Helical; Name=2, Helical; Name=4 membrane-spanning region. The designed protein sequence is ",
    "response#1": "GNLLILLVIRVDSHLHTTMYYFLTNLSFIDMWFSTVTVPKLLMTLVFPSGRAISFHSCMAQLYFFHFLGGTECFLYRVMSCDRYLAISYPLRYTSMMTGRSCTLLATSTWLSGSLHSAVQAILTFHLPYCGPNWIQHYLCDAPPILKLACADTSAIETVIFVTVGIVASGCFVLIVLSYVSIVCSILRIRTSEGKHRAFQTCASHCIVVLCFFGPGLFIYLRPGSRKAVDGVVAVFYTVLTPLLNPVVYSLRNQEVKAAVSR",
    "response#2": "GNLGLIVLIWNDPHLHMPMYLFLGGLAFSDACTSTSITPRMLVNFLDKTAMISLAECITQFYFFASSATTECFLLVMMAYDRYVAICNPLLYPVMMSNKLSAQLLSISYVIGFLHPLVHVSLLLRLTFCRFNIIHYFYCEILQLFKISCNGPSINALMIFIFGAFIQIPTLMTIIISYTRVLFDILKKKSEKGRSKAFSTCGAHLLSVSLYYGTLIFMYVRPASGLAEDQDKVYSLFYTIIIPLLNPFIYSLRNKDVKNALKRLLTC",
    "response#3": "GNLLIVLAICSDVHLHNPMYFLLANLSLVDIFFSSVTIPKMLANHLLGSKSISFGGCLTQMYFMIALGNTDSYILAAMAYDRAVAISRPLHYTTIMSPRSCIWLIAGSWVIGNANALPHTLLTASLSFCGNQEVANFYCDITPLLKLSCSDIHFHVKMMYLGVGIFSVPLLCIIVSYIRVFSTVFQVPSTKGVLKAFSTCGSHLTVVSLYYGTVMGMYFRPLTNYSLKDAVITVMYTAVTPMLNPFIYSLRNKELRGAMKRLLGRAPG"
  },
  {
    "reference": "MDVGRCFLFLLLPSFFFLPSQTQSTDSFTSVLVSQNGLDFVKNLLVNKAIASIIPLQIPRIEKSMKIPFLGGIDVVVSNLTIYELDVASSYVKLGETGVVIVASGTTCNLSMNWHYSYSTWLPPIEISDQGIASVQVQGMEIGLSLGLKSDEGGLKLSLSECGCHVEDITIELEGGASWFYQGMVNAFKDQIGSSVESTIAKKLTEGVSDLDSFLQSLPKEIPVDDNADLNVTFTSDPILRNSSITFEIDGLFTKGETNQVLKSFFKKSVSLVICPGNSKMLGISVDEAVFNSAAALYYNADFVQWVVDKIPEQSLLNTARWRFIIPQLYKKYPNQDMNLNISLSSPPLVKISEQYVGANVNADLVINVLDANQVIPVACISLMIRGSGALRVMGNNLGGSVSLEDFSMSLKWSNIGNLHLHLLQPIVWTVIQTVFVPYANDHLEKGFPLPIMHGFTLQNAEIICSESEITVCSDVAYLDSSQQPQWL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Include a signal peptide as an essential component of the protein.2. The designed protein should have lipopolysaccharide binding within plant-type vacuole, extracellular space to regulate reactive oxygen species biosynthetic process, regulation of gene expression. The designed protein sequence is ",
    "response#1": "PLKKYRKEELETLQGKNREEVGEFTKFERIYDYDVYNDVGDPDNDPELARPVIGGLTHPYPRRCKTGRKPCETDPSSEQRYGGEFYVPRDEEFSTAKGTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFEEGIQLPKDAGLLPLLPRIIKALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTLAGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEALKNKRLFVLDYHDLLLPYVNKVRELNNTTLYASRTLFFLSDDSTLRPVAIELTCPPNINKPQWKQVFTPGYDATSCWLWNLAKTHAISHDAGYHQLISHWLRTHACTEPYIIAANRQLSAMHPIYRLLHPHFRYTMEINARARQSLVNGGGIIETCFWPGKYALELSSAVYGKLWRFDQEGLPADLIKRGLAEEDKTAEHGVRLTIPDYPFANDGLILWDAIKEWVTDYVKHYYPDEELITSDEELQGWWSEVRNIGHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSGHHAAVNFGQYGYGGYFPNRPTTTV",
    "response#2": "SQIGKELTNTFLTTLWNDLEHPPISYLGRDAMYRRADGSGNNVLWPHIGAAGTPYARSVQPKTVQSPNLPDPETLFDCLLARKEYKEHPNKISSVLFYIASIIIHDLFETDRKDPAISLTSSYLDLSPLYGNNQQEQDLIRTFKDGKLKPDCFSTKRVLGFPPDVGVVLIMFNRFHNYVVEKLAMINEGGRFTKPQESDTAAYAKYDNDLFQTGRLVTCGLYVNIILKDYVRTILNINRTDSIWSLDPRSEMKDGLLGRAAAQATGNQVAAEFNLVYRWHSCISQRDQKWTEDMYQELFPGQDPSKISLQDFLRGLGRWEAKLPGEPRERPFAGLQRKADGSYDDNDLVKIFEESVEDCAGAFGALHVPTVFRSIEALGIQQARSWNLATLNEFRKYFNLAPYKTFEEINSDPYVADQLKRLYDHPDRVEIYPGIIVEDAKESMAPGSGLCTNFTISRAILSDAVALVRGDRFHTVDFTPKHLTNWAYNEIQPQDSVDQTHVFYKLVLRAFPNHFRGDSIYAHFPLVVPSENKKILTKLGTADKYSWDRCAR",
    "response#3": "SQIGKELTNTFLTTLWNDLEHPPISYLGRDAMYRRADGSGNNVLWPHIGAAGTPYARSVQPKTVQSPNLPDPETLFDCLLARKEYKEHPNKISSVLFYIASIIIHDLFETDRKDPAISLTSSYLDLSPLYGNNQQEQDLIRTFKDGKLKPDCFSTKRVLGFPPDVGVVLIMFNRFHNYVVEKLAMINEGGRFTKPQESDTAAYAKYDNDLFQTGRLVTCGLYVNIILKDYVRTILNINRTDSIWSLDPRSEMKDGLLGRAAAQATGNQVAAEFNLVYRWHSCISQRDQKWTEDMYQELFPGQDPSKISLQDFLRGLGRWEAKLPGEPRERPFAGLQRKADGSYDDNDLVKIFEESVEDCAGAFGALHVPTVFRSIEALGIQQARSWNLATLNEFRKYFNLAPYKTFEEINSDPYVADQLKRLYDHPDRVEIYPGIIVEDAKESMAPGSGLCTNFTISRAILSDAVALVRGDRFHTVDFTPKHLTNWAYNEIQPQDSVDQTHVFYKLVLRAFPNHFRGDSIYAHFPLVVPSENKKILTKLGTADKYSWDRC"
  },
  {
    "reference": "MARTWLLLFLGLRCQALPSGIAGTPFPSLAPPVTLLVDGRRHTLVVCLVLDAAPPGLDSLVWFSGGNGSALDAFTYGPSPAPDGTWTSLGQLSLSSEELEAWEPLVCHTRPAAGGLNRSTHPLQLSGEEASTDRTCPQETLRGTQRQVLRLSVLRLLLFKLLLLDVFLTCSRLCVLAGQHLLPPPSSKQAPASTHQSWT",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The Extracellular topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.3. The protein should be optimized for stability and solubility when expressed in membrane where it will be involved in thymocyte apoptotic process, negative regulation of thymocyte apoptotic process.4. Design the protein with a signal peptide incorporated.5. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "VMLECHSGGWFPQPHMEWRDNKGNIIPATSKAHSQDENKLFNMTMTLLIEASSHRSITCYLQNLLTHQEESIC",
    "response#2": "PMLEAYDNAVNLTCKYSCNLFSRQFQASLHKGVDSAVQVCVVHGNYSQLLQVNSATGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEIMYPPPYLI",
    "response#3": "PMLEAYDNAVNLTCKYSCNLFSRQFQASLHKGVDSAVQVCVVHGNYSQLLQVNSATGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEIMYPPPYLL"
  },
  {
    "reference": "MEFQLLLLCSVWALGVCAEQEVENLSGLSTNPERDIFVVRENGTTCLMAEFAVRFLIPYDVLALNGIDLITEQTSVAIPRGAQIAGKCGPSESELQISWAAQAFNFHIYFSKEKRVQGSDGKAPEAEVWKINRVQLVYDTSENTHFINAYNPGKHTASTHRLSALVTPAGRSYVCAAQQTLTLISSDHQKGVTVSIYDIQIQPFDINADFVFSEPYKCVTDQREQLEQTLPLVLGLILGLIIVITISVYHFHLKLNAAHTQQPTLPRDRSLYKNM",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. For general function, the protein need meet that Plays a role in short-term synaptic plasticity in a subset of GABAergic neurons in the brain.3. The Cytoplasmic, Extracellular topological domain of the protein should be designed to be flexible or rigid.4. Incorporate a signal peptide in the protein design.5. The protein should be designed to localize specifically to dendrite membrane, early endosome membrane, late endosome membrane, lysosomal membrane, endoplasmic reticulum-Golgi intermediate compartment membrane in order to perform its role in establishment of protein localization to organelle more effectively. The designed protein sequence is ",
    "response#1": "IRQGKEATAVVDQILAQEENWKFEKNNEYGDTVYTIEVPFHGKTFILKTFLPCPAELVYQEVILQPERMVLWNKTVTACQILQRVEDNTLISYDVSAGAAGGVVSPRDFVNVRRIERRRDRYLSSGIATSHSAKPPTHKYVRGENGPGGFIVLKSASNPRVCTFVWILNTDLKGRLPRYLIHQSLAATMFEFAFHLRQRISELGAQ",
    "response#2": "MQNYSSNFTSRLFVSTDYGKTYDNVSATSKLRLADGRVAKIHSCVKSPSSTNHYVFTDVENKVLFTTADGCQTFMRRNPPFVPFEINVDPNNSDVLFGREEKDLKRRLWLSVDFGTSWKAVEEYVMAAIFEKHPAFDLNGSTTMYITRMKPNGKQDLLRSSTYFMNDAKTEKLIADVEEFEMMGNFLFATKNESAEPGRKSTLSLWVSYQRKPFQRADFGFGSDRTVKDYFVSNATDVQAVVAVTFDNNQSDIYFSAPGSVSFSRSLENVLFYHPSPSIGKFQSLKDFWNHPKPFAEIYSVKGLRGVFIANQLVKDETGHLGNSVSLISFDNGASWQKIRPPRMDRDGVPTKRDSYIQLMQTFPVFSPAFSQGLFSSESAPGLIIGSGVISESLIGGEQHSVFVSTTGGTTWTESLRGVYKFAVGDHSGLIVAVNGLGRTSDLLYSWDEGLTWQTLRISEKKLRVISIMTEMNGISPVFHLFGTLMEKDNRTIHEWQIIRVNLTKIFDGKKCEDKDFKDWSFEDRIGHRCVMGKRINYSRKNPGSKCLMGHDFKRKTVTENCTCQYYDYECDLGFRPSRTVGRELACEKDNSSTYDPTKTPVTCPPGSFWMRSRKYRKIPMNTCNGGPGSKRYEPEKVACPWADP",
    "response#3": "MQNYSSNFTSRLFVSTDYGKTYDNVSATSKLRLADGRVAKIHSCVKSPSSTNHYVFTDVENKVLFTTADGCQTFMRRNPPFVPFEINVDPNNSDVLFGREEKDLKRRLWLSVDFGTSWKAVEEYVMAAIFEKHPAFDLNGSTTMYITRMKPNGKQDLLRSSTYFMNDAKTEKLIADVEEFEMMGNFLFATKNESAEPGRKSTLSLWVSYQRKPFQRADFGFGSDRTVKDYFVSNATDVQAVVAVTFDNNQSDIYFSAPGSVSFSRSLENVLFYHPSPSIGKFQSLKDFWNHPKPFAEIYSVKGLRGVFIANQLVKDETGHLGNSVSLISFDNGASWQKIRPPRMDRDGVPTKRDSYIQLMQTFPVFSPAFSQGLFSSESAPGLIIGSGVISESLIGGEQHSVFVSTTGGTTWTESLRGVYKFAVGDHSGLIVAVNGLGRTSDLLYSWDEGLTWQTLRISEKKLRVISIMTEMNGISPVFHLFGTLMEKDNRTIHEWQIIRVNLTKIFDGKKCEDKDFKDWSFEDRIGHRCVMGKRINYSRKNPGSKCLMGHDFKRKTVTENCTCQYYDYECDLGFRPSRTVGRELACEKDNSSTYDPTKTPVTCPPGSFWMRSRKYRKIPMNTCNGGPGSKRYEPEKVACPWADV"
  },
  {
    "reference": "MDVLLSLLSEFGFERLSSELSLPIVVHSVPGGGKSSLIRKLINKDRRFSAYTFGLEDCESITGVRIKKAHASIPRSEFVVFDEYIEGDTPPWAFAVFADPLQGGPGPVLRAHFIKRRSHRFGKCTAQLLNDLSYEVESDLADVVQIQGLYETDLQGTVVYYEACVGNLLRAHSVPAYYISEIRGQTFESVTFVTSENYPVDRALAFQCLTRHRSSLLILSPNATYTAS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein have (+)RNA virus helicase C-terminal, (+)RNA virus helicase ATP-binding domains with specific sequence motifs that are crucial for its activity.2. The designed protein should have RNA binding within host cell cytoplasm to regulate transport of virus in host, cell to cell.3. The protein must exhibit the following characteristics: Transports viral genome to neighboring plant cells directly through plasmosdesmata, without any budding; Increases plasmodesma size exclusion limit; The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier; Acts as a suppressor of RNA-mediated gene silencing, also known as post- transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs .. The designed protein sequence is ",
    "response#1": "MKTDLLLQILSNNNFTRTSEPIKEPLIIHGVPGSGKSTLVRALVTYRSTVACTLGAPYGSNLAFPGVTSPGLTQSLTDHETRILDEYQLGTESDLKPFNVLVGDPFQGNLHLKAHYVKSFSHRVPRIICNFLQSLGYEIAGSKPGELAQLPIYGPNPSGPTGQVLHLGPLSRRLTQSHGVCSKLPSEVQGLEFEEVTLVYHSSEFERNRVGFYIAATRALGRLNLITDTTLEIPHELCPTLVVHSVAGSGKSTLIRKFLEEQPLARAYTHCRADPPNLEGRFIQPFKGPCPDHFNILDEYCKEPISAKFQVLIADPLQYRTQHLRPHYVNHKSHRLGPETCKLLSSLGIKVESHRRDRDVVTLSGIFGSPILGQAIALDRSASDLLRAHGIQALCPIESIGQEYPVVTVVSSEPLRNVRFKDQVYIALSRHTEQLHVL",
    "response#2": "PDVATCRFYTLDSVSWMKESQGWWWKFPDALREMGLFGQNMQYHYLGRSGYTIHVQCNASKFHQGCLLVVCVPEAEMGAAKVNEKINREHLSNGEVANTFTSTKSSNTNDVQQAVFNAGMGVAVGNLTVFPHQWINLRTNNCATIVMPYINSVPMDNMFRHYNFTLMIIPFAKLDYAAGSSTYIPITVTVAPMCAEYNGLV",
    "response#3": "MKTDLLLQILSNNNFTRTSEPIKEPLIIHGVPGSGKSTLVRALVTYRSTVACTLGAPYGSNLAFPGVTSPGLTQSLTDHETRILDEYQLGTESDLKPFNVLVGDPFQGNLHLKAHYVKSFSHRVPRIICNFLQSLGYEIAGSKPGELAQLPIYGPNPSGPTGQVLHLGPLSRRLTQSHGVCSKLPSEVQGLEFEEVTLVYHSSEFERNRVGFYIAATRALGRLNLITDTTLEIPHELCPTG"
  },
  {
    "reference": "MSKKLGNNSKKIAFVHPDLGIGGAERLVVDAAVGLQELENEVTIYTSHCDKKHCFEEVSSNLLDVEVYGDFFPTNVLKRFHILFAIIRQFYLVLALIFTGKIKQYDYFIVDQLSFCIPLLCCFSRPECKILFYCHFPDQLLALKGGFLKRFYRMPFDLIEEWTTGISDQIVVNSKFTKGIFHKTFKGLKNIEPGVIYPCVDLNSATDTEEDKLMDEEVNEFFKGGKFFLSVNRFERKKNIGLAIQSFAKFKAQLPKNVSEDNRIKPRLVVAGGFDPRVLENVEYLQELNGLSESLNLKCFTIRGKLLIIPPATDILFLPSIKSSLKKSLIKNAELLLYTPSFEHFGIVPVESMLFKTPVLSANNGGPLESIVHFTSDNIATATGYSQEPNDELWSKTMHTFYTELDEATKLKLGENGLTRVHELFSRHQMSEAFMQNLIQSNSKDEEKGILYGILKMWRIELLFVLISYYLVRLYK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be designed to have high stability and activity towards alpha-D-Man-(1->3)-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-   alpha-D-GlcNAc-diphosphodolichol + GDP-alpha-D-mannose = alpha-D-Man-   (1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-   alpha-D-GlcNAc-diphosphodolichol + GDP + H(+).2. The protein must exhibit the following characteristics: Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate.. The designed protein sequence is ",
    "response#1": "IGGAERWVVDAAVGLQNLGHEVDIYTSYCNKSHCFDEVRDGLLKVTVLGDTICPHTIKGKFAIFCATFRQLHLAYELKKGPGSKVDVFVVDQLSACVPLLKLWFPKARVLFYGHFPDQLLVQNRNQMSLVKKAYRYPFDKFEEITTASADRLVVNSHFTKDMFEKTFPATKNPLVIYPCVDTLQDEPELQRKLLISVNRFERKKDIRLAIDAFSALRDLSANRFPEYLLLVAGGYDIRVSENRRYLKELQEFCEQKDLSYTTVKDNWDNITVAPSTNVLFLLSVPSKVRDALISSSRILLYTPENEHFGIVPLEAMLRKVPVLAQTNGGPLETVIDGKNGWLASQYTFLLSINRFERKKNILLAIEAFGEAQKKSSNLKLAVAGGYDFRVNENVEYLQELILACEKLKAKNDVIFFPSASNSFKNTLLHISKLLLYTPQNEHFGIVPLEGMLWKTPVLATNSGGPLETVKDNVGWTVEGKSELWAPVIDKVVHMKNDVIFFPSASNSFKNTLLHISKLLLYTPQNEHFGIVPLEGMLWKTPVLATNSGGPLETVKDNVGWTVEGKSELWAPVIDKVVHMASQYTFLLSINRFERKKNILLAIEAFGEAQKKSSNLKLAVAGGYDFRVNENVEYLQELILACEKLKADYKQHD",
    "response#2": "IGGAERLVVDAAVGLQSLGKEVVVFTSHCDKKHCFEEIRDGTIKVKVYGDWLPSSIFGRLSIFCSSLRQVYLTMILLTNYMHFDAIIVDQLSTCVPFLLLASQMILFYCHFPDKYLAKRGGILKKLYRIPFDTVEAESVRLADRIVVNSKFTASVFKKAFPKIRKPLRIVHPCVDIIGGAERWVVDAAVGLQNLGHEVDIYTSYCNKSHCFDEVRDGLLKVTVLGDTICPHTIKGKFAIFCATFRQLHLAYELKKGPGSKVDVFVVDQLSACVPLLKLWFPKARVLFYGHFPDQLLVQNRNQMSLVKKAYRYPFDKFEEITTASADRLVVNSHFTKDMFEKTFPATKNPLVIYPCVDTEQYFAKNDVIFFPSASNSFKNTLLHISKLLLYTPQNEHFGIVPLEGMLWKTPVLATNSGGPLETVKDNVGWTVEGKSELWAPVIDKVVHMKNDVIFFPSASNSFKNTLLHISKLLLYTPQNEHFGIVPLEGMLWKTPVLATNSGGPLETVKDNVGWTVEGKSELWAPVIDKVVHMLDKNDVIFFPSASNSFKNTLLHISKLLLYTPQNEHFGIVPLEGMLWKTPVLATNSGGPLETVKDNVGWTVEGKSELWAPVIDKVVHMKNDVIFFPSASNSFKNTLLHISKLLLYTPQNEHFGIVPLEGMLWKTPVLATNSGGPLETVKDNVGWTVEGKSELWAPVIDKVVHMGNVTEKAWTTARGAIKLP",
    "response#3": "IGGAERWVVDAAVGLQNLGHEVDIYTSYCNKSHCFDEVRDGLLKVTVLGDTICPHTIKGKFAIFCATFRQLHLAYELKKGPGSKVDVFVVDQLSACVPLLKLWFPKARVLFYGHFPDQLLVQNRNQMSLVKKAYRYPFDKFEEITTASADRLVVNSHFTKDMFEKTFPATKNPLVIYPCVDTGVDEKILLQRKLLISVNRFERKKDIRLAIDAFSALRDLSANRFPEYLLLVAGGYDIRVSENRRYLKELQEFCEQKDLSYTTVKDNWDNITVAPSTNVLFLLSVPSKVRDALISSSRILLYTPENEHFGIVPLEAMLRKVPVLAQTNGGPLETVIDGKNGWLKNDVIFFPSASNSFKNTLLHISKLLLYTPQNEHFGIVPLEGMLWKTPVLATNSGGPLETVKDNVGWTVEGKSELWAPVIDKVVHMKNDVIFFPSASNSFKNTLLHISKLLLYTPQNEHFGIVPLEGMLWKTPVLATNSGGPLETVKDNVGWTVEGKSELWAPVIDKVVHMSKNDVIFFPSASNSFKNTLLHISKLLLYTPQNEHFGIVPLEGMLWKTPVLATNSGGPLETVKDNVGWTVEGKSELWAPVIDKVVHMSE"
  },
  {
    "reference": "MMKIKPVKVESIENPKRFLNSRLLTKIEVEEAIEKALKQLYINIDYFGEEYPTPATFNNIYKVMDNTEWTNGFWTGCLWLAYEYNQDKKLKNIAHKNVLSFLNRINNRIALDHHDLGFLYTPSCTAEYRINGDVKALEATIKAADKLMERYQEKGGFIQAWGELGYKEHYRLIIDCLLNIQLLFFAYEQTGDEKYRQVAVNHFYASANNVVRDDSSAFHTFYFDPETGEPLKGVTRQGYSDESSWARGQAWGIYGIPLSYRKMKDYQQIILFKGMTNYFLNRLPEDKVSYWDLIFTDGSGQPRDTSATATAVCGIHEMLKYLPEVDPDKETYKYAMHTMLRSLIEQYSNNELIAGRPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDWELYW",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The design objective is to achieve a protein with helix-helix-strand-helix-helix-turn-helix-helix-turn-helix-helix-helix-helix-helix-turn characteristics.2. The designed protein should have a high specificity for substrate binding, with minimal off-target effects.3. A protein with unsaturated chondroitin disaccharide hydrolase activity, chondroitin hydrolase activity is needed to enhance polysaccharide catabolic process in a specific context.4. The protein must exhibit the following characteristics: Releases 4-deoxy-4,5-didehydro D-glucuronic acid or 4- deoxy-4,5-didehydro L-iduronic acid from chondroitin disaccharides, hyaluronan disaccharides and heparin disaccharides and cleaves both glycosidic (1->3) and (1->4) bonds; Prefers sulfated glycosaminoglycans compared to unsulfated glycosaminoglycans.5. The designed protein must have a Nucleophile that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "ERYILVDIDDIFVGKEGTRMKVSDVKALLETQNELRNHVPNFTFNLGFSGKFFHAGTDEEDLGDDLLLSYSKEFWWFPHMWSHMQPHLFHNQSVLAEQMLLNHKFAEEHGIPTNMGYAVAPHHSGVYPIHLQLYEAWKKVWGIKVTSTEEYPHLKPARFRRGFVHSGISVLPRQTCGLFTHTIFYNEYPGGSKELDKLIYGGELERYILVDIDDIFVGKEGTRMKVSDVKALLETQNELRNHVPNFTFNLGFSGKFFHAGTDEEDLGDDLLLSYSKEFWWFPHMWSHMQPHLFHNQSVLAEQMLLNHKFAEEHGIPTNMGYAVAPHHSGVYPIHLQLYEAWKKVWGIKVTSTEEYPHLKPARFRRGFVHSGISVLPRQTCGLFTHTIFYNEYPGGSKELDKLIYGGELVFEAINLNYPGLEKVKEFYEAGEHYYAANALLEYYRTRTNVTNPNLSLINVTISEAEQAKADYALVDYRFHVNNFYEDKETLKPYSVKQDGGINWEYSPKDASDEYQKQLHRHQWFIPQAKAYRVSGDEKYIQSWIEVYKNWIENNPKPTTGPNTTSWWQLQVSTRIGDQVQLLEYFKNSVNFTPEWLSTFLVEFAEQADFLVDYPYESGGNILISQANALATAGTLMPEFKNAEKWMNTGYQILSEEVQNQIMSDGWHKEMSLHYHIGIVADFYEAMKLAEANQLSSKLPSDFTEPLRKAAEVVMYFTYPNYFIKGSDNVVPMFNDSWSRTRNVLKNTNFKQYVEMFPDSEELK",
    "response#2": "TDPVVLVFVESLYSQLGQDIVAILESGRFRYQTEIAPGKGDMPTLTDKNRGRFTLVIYENILKYVNLDAWNRELLDKYCVEYGVGIIGFFKANENSLQSAQLKGFPLFLHSNLGLRDCSVNPKSPLLFITKAREVEHGPLPGDDWTVFQSNHTTYEPVLLARGRSTEASGAPGPLHAAVVQDLGLHDGIQRVLFGNNLNFWLHKLVLVDAVSFSEEPVSPPPAPDLLIIGPQKTGSTALASFLALHPNVSQNMEIPGSFEEIQFFSGQNYLKGVEWYMSKFPNETTVIFEKSATYFDNPSAARQAAAMVPHAKLVIILQNPTQRAYSWFQSLFQHLIAHKDPIAMSSESLDVILNSTSSESAKFKIRQRCLSGGRYVHHLDKWLEHFSLQQIQFIDSDELRKEPAKQSWLAIARGFSRYLVGNESYENNNRYGRYLQYGQLEIIPADLTQSGFSHAGWDWNRYPGTTTIHLPYNELEAKLNQLPAAGIEEMLLSTESYSGANTLNNNSMFAMKLHGHSKYQQQSLRANKSYFLFDNRVIALGSGIENDDKQHTTETTLFQE",
    "response#3": "TDPVVLVFVESLYSQLGQDIVAILESGRFRYQTEIAPGKGDMPTLTDKNRGRFTLVIYENILKYVNLDAWNRELLDKYCVEYGVGIIGFFKANENSLQSAQLKGFPLFLHSNLGLRDCSVNPKSPLLFITKAREVEHGPLPGDDWTVFQSNHTTYEPVLLARGRSTEASGAPGPLHAAVVQDLGLHDGIQRVLFGNNLNFWLHKLVLVDAVSFERYILVDIDDIFVGKEGTRMKVSDVKALLETQNELRNHVPNFTFNLGFSGKFFHAGTDEEDLGDDLLLSYSKEFWWFPHMWSHMQPHLFHNQSVLAEQMLLNHKFAEEHGIPTNMGYAVAPHHSGVYPIHLQLYEAWKKVWGIKVTSTEEYPHLKPARFRRGFVHSGISVLPRQTCGLFTHTIFYNEYPGGSKELDKLIYGGELRTWAGSGQSWLAIARGFSRYLVGNESYENNNRYGRYLQYGQLEIIPADLTQSGFSHAGWDWNRYPGTTTIHLPYNELEAKLNQLPAAGIEEMLLSTESYSGANTLNNNSMFAMKLHGHSKYQQQSLRANKSYFLFDNRVIALGSGIENDDKQHTTETTLFQELQSGPPYECGGVFTDPKQIFKSPGFPNEYEDNQICYWHIRLKYGQRIHLSFLDFDLEDDPGCLADYVEIYDSYDDVHGFVGRYCGDELPDDIISTGNVMTLKFLSDASVTAGGFQIKYVAGEDNEEKHVSRATIYQPTTSQSWLAIARGFSRYLVGNESYENNNRYGRYLQYGQLEIIPADLTQSGFSHAGWDWNRYPGTTTIHLPYNELEAKLNQLPAAGIEEMLLSTESYSGANTLNNNSMFAMKLHGHSKYQQQSLRANKSYFLFDNRVIALGSGIENDDKQHTTETTLFQ"
  },
  {
    "reference": "MSTKVVAVVGATGVVGRQMLETLEARNFPLSRLVPLASERSRGKTVSFKGETIPVEVLSENSFEGIDIALFSAGGGTSKKYAPLAAASGAVVIDNSSAFRMDPDVPLVVPEVNAHALKNHKGIIANPNCSTTQMVVVLKPLHDAARIRRIVVSTYQAVSGAGIAAITELQEQSRALLAGEPYAPKVFPHQIAFNCLPQIPQSDAFLDNGYTSEEMKMVHETRKILGDDSIAVTATTVRVPVLNGHSESVNVQTEKKLTAAEARALLSKAPGVVVQDDPQHQIYPLAVNSTGKFDTFVGRIRDDISHESALDMWIVSDNLLKGAALNTVQIAEELL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A key design requirement for the protein is the optimization of the Proton acceptor, Acyl-thioester intermediate to achieve high catalytic efficiency for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.2. The designed protein have Semialdehyde dehydrogenase NAD-binding domains with specific sequence motifs that are crucial for its activity.3. The designed protein should have protein dimerization activity, aspartate-semialdehyde dehydrogenase activity, NADP binding, NAD binding to facilitate isoleucine biosynthetic process, diaminopimelate biosynthetic process, lysine biosynthetic process via diaminopimelate, threonine biosynthetic process.4. Ideal protein should have a binding affinity for key enzymes in the L-threonine from L-aspartate: step 2/5, Amino-acid biosynthesis pathway to enhance the overall efficiency of the pathway.5. The phosphate, NADP(+), substrate binding site should be located in a region of the protein that is accessible to the ligand.6. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.. The designed protein sequence is ",
    "response#1": "MTNLALVGATGAVGTVMIGIINSRPNIWGEIRLIASARSAGKKITVHGEELTVVALSPEAFDGIDIAMFDVPDEVSEVWAPIAAERGAVAVDNSGAFRMDPDVPLVVPEVNPEQTKNLKPIYDAVGIARINVCTYQAVSGTGKEAIAELVTQTANLLNGKPIVAEVYPKQIAFNVLPQIDVFMDNGYTKEEMKMVWETRKILGDDSILVNPTAVRVPVFFGHSEALHIETKEKITVEAVRALLAKAPGVRLLDERKDGGYPTAVTESSGHDEVFVGRIREDISHPNGIDLWVVGDNVRDAVIANPNCSTILMVVAALHDKWKLEELVVSSYQAVSGAGAVGIDELALEMGIVGKDPSLGTNTDDVRKALAAAGVDLSDSPWAHPIALNVIPFAGSLKEGGHSSEELKVRNESRKILGISDLKVAASCVRVPVQVSHSLTVHAKFANPLTPDEVREVLRKQPTIRVVDEPEKQLYPTPAMVVGQDPTFVGRIRQSLDFPNSIELFVVGDNLRKGAATNAVQIAQRIVEMGGV",
    "response#2": "MKVAIVGASGAVGQEFLRVLEERNFPVDELLLFGSGRSAGTMYNFRGKSITVKELRHNDDFKDVDIALTSAGASVSKEFASTITKYGAVMIDNSSAFRMDDDVPLVVPEVNPEDCKLAPLHQKYAVKRLVISTYQSITGTGVKAVEQLENEYAGKKGAMAYPYQIHKNAIPHCDVFEDNGYTKEEMKLVRETQKILNDRTIAVTATAVRIPVVGGHSESVNVEFRNEFDVSDIRRLLSETSGITVQDNVDVNVYPMPLLAHGRDDVFVGRIRRDHSQENTLNMWIVSDNLRDGIRRNIIANPNCSTIQMVVALAPLSKLATIKRVVASTYQSASGAGAQAMQELLDQTADYLAGRSIEPRIMPHPYAFNLFSHNTAIGEDGYNGEEAKVIEESRKILSMPELALTVTCVRVPVLRAHSISVNVEFDRPVDESEAREVLLASQGVRVVDDREHNLFPMPSEASGQDETLVGRIRQDNTNANALNLFVCGDQLRKGAALNAIQIAEVLIRNNLV",
    "response#3": "MAKVAIVGISGAVGQEFLQVLQERRFPVDELLLFGSARSAGNRYAFQGEQLTVKELKDNDDFKGVHIALVSAGGNTSRQFASAITRHGAIMIDNSSAFRMEEDVPLVIPGVNSGDLLKPIHDLSKIKRVHVATYQAASGAGAAAMRELEIQLKQVLNGEQPTIEKFKYQLAFNLIPHIDVFLENDYTKEEMKMHNETRKIMHAGIDVSATTVRVPVLRAHSEAVWVETEKELTVEEVRTALMKADGVVVEDDPKNYRYPMPLFVAGKDPVYVGRIRKDITNPKGITFFCVGDQLKPIYDAVGIARINVCTYQAVSGTGKEAIAELVTQTANLLNGKPIVAEVYPKQIAFNVLPQIDVFMDNGYTKEEMKMVWETRKILGDDSILVNPTAVRVPVFFGHSEALHIETKEKITVEAVRALLAKAPGVRLLDERKDGGYPTAVTESSGHDEVFVGRIREDISHPNGIDLWVVGDNVRLKPIYDAVGIARINVCTYQAVSGTGKEAIAELVTQTANLLNGKPIVAEVYPKQIAFNVLPQIDVFMDNGYTKEEMKMVWETRKILGDDSILVNPTAVRVPVFFGHSEALHIETKEKITVEAVRALLAKAPGVRLLDERKDGGYPTAVTESSGHDEVFVGRIREDISHPNGIDLWVVGDNVRLKPIHDLSKIKRVHVATYQAASGAGAAAMRELEIQLKQVLNGEQPTIEKFKYQLAFNLIPHIDVFLENDYTKEEMKMHNETRKIMHAGIDVSATTVRVPVLRAHSEAVWVETEKELTVEEVRTALMKADGVVVEDDPKNYRYPMPLFVAGKDPVYVGRIRKDITNPKGITFFCVGDQ"
  },
  {
    "reference": "MATTVADVMVATLKASGVRRVYGIPGDSLNGFTDALRRDGAISWQLVRHEEAAGFAAAGEAALTGELAVCAGSCGPGNLHLINGLYDANRSRVPVVAIAAHIPREEIGGSYFQETHPELLFRECSVYCELASIPAQLPRLLELAMRAALQHRGVAVIIVPGEIFLGEASGGATPAPVRAVWPVVRPDEPALADAARVLDAASRVTILAGAGCAGAHDQLVGLAAALRAPVVHAMRGKDVVEPDNPYDVGMTGLIGFSSGYRAMEHCDALVMLGTDFPYRPFLPEGVPVIQVDVRGEQIGRRVPVQVPLVGTVRDTIDALLPLIAVKTDSTHLDRMTAHYRRARTRLDRLARPGRDGAPLHPQYIAATIDRLAAADAVFTADVGTPSIWAARYLHMNGARRLIGSFNHGSMANALPQAIGAQAAAPGRQVIALSGDGGLAMLLGELITLRQQRLPVKVVVFTNGALSFVELEMKAGGIVTYGTELDNPDFAGIARAAGLFGARVESASELEETLAAAFAHDGPALVDVHTARHELALPPKLTYGQMKGFTLYATRTIVSGGGDELVELARTNLRDLDAE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be involved in pyruvate catabolic process by localizing to plasma membrane and exhibiting lipid binding.2. The protein should be able to bind thiamine diphosphate, Mg(2+) ligand in a variety of conditions3. For general function, the protein need meet that A peripheral cell membrane enzyme that catalyzes the oxidative decarboxylation of pyruvate to form acetate and CO(2)4. The presence of Thiamine pyrophosphate enzyme central, Thiamine pyrophosphate enzyme N-terminal TPP-binding domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "VQQAAAILNDAGAVTILAGAGVEGSHDAVITAATTLNAPIVHALRGKEFIEHDNPHDVGMTGLLGFPSGYKAIKEADALLMLGTDFPYQQFYPDNAKIIQVDIRGRNLGRRTPIELGLVGSVRDTLAAL",
    "response#2": "RAAAAILNDAHAVTVLGGAGVEGAHDALVTLAATLKAPVVHALRGKEFIEYDNPYDVGMTGLLGFASGYKAIKEADTLLMLGTDFPYQQFYPDHANVIQVDIRGRNLGRRTPIDLGLVGTVADTLAAVRAVLG",
    "response#3": "LDKLAALLGSGRKVTLFCGRGCAGAHDEVIRLAETLKAPVVHALGGKEHVEWDNPYDVGMTGLIGFSSGYEAMMNCDTLLLLGTDFPYRQFFPDDAKICQIDLRAENLGRRCHLDLGLVGDVGATIAAHQAAALLNGAQKVTILGGAGCEGAHDELVALAAKLKAPIVHALRGKEFIEYDNPYDVGMTGLLGFSSGYHAMMNCDALLMLGTDFPYPQFYPKKAKILQVDLRGEQIGRRTPVDLGLVGNVKDTLDAVRAAVGAL"
  },
  {
    "reference": "MAENRWKIHKFGGSSLADAACFRRVAGIVLSYSDARIGVVVSAMGGMTDALINLAILAEQDDDHFLDQLHAIGERYAKTASALVDGDRRVSVLDAWGRDAEDITDVLKAIALVKSAPRRSRDVIAGYGEIWSARLLAACLEQEAPERGGTWIDARDVVTVHQTELGPSVLWEASRKKFADVVPENFAGIAVITGFIATDEDGLQTTLGRNGSDYSAAIFAALSNASELSIWTDVDGVMSADPRRVPEAQVIDELTYNEAMELAYFGANVIHPQTLGPAIDSDIPVVIRSSHNPSHPGSRIGKQAERVENIKGITAIGGMALVNLEGSGMIGVPGTADRLFAALKNAGVSVTLISQASSEHSICIAVPEDVAERAGKVITAAFADELESGQIQSVEVTKAQSIVAVVGDGMAGTPGIAARFFGTLGRAGINIRAIAQGSSERNISAVVDSEDATRALRAAHSGFYLSAKTLSIGLIGPGTVGTSLLRQIEKQAQRLHDEFNLDLRVRAIARSQTMLLGDRSIDLARWQQDLAESGAACDLGKLEAHINPDHLPHAVIIDCTASEHIAGRYSGWLERGIHIITPNKKAFSGPFENYVALRESAAHGNSHCFYETTVGAALPIITTLRDLIDTGDEVHLLQGIFSGTLAYLFNVYDGTTPFSEIVRTARTSGYTEPDPRDDLSGMDVARKLTILARELGQTIEIGDFPVQNLVPEQLRDCSLEEFLDRLPEVDADMKALYETAQEKGEKLRYVGRLSADGTATVGLAAVGS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have ATP binding, aspartate kinase activity, NADP binding, homoserine dehydrogenase activity to facilitate threonine biosynthetic process, lysine biosynthetic process via diaminopimelate.2. The protein contains novel ACT domains that confer a unique function or activity.3. A protein that can modulate the overall flux of metabolites through the L-threonine from L-aspartate: step 3/5, L-threonine biosynthesis, Amino-acid biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MRVLKFGGTSLANPERFSQAAQLIEQAHLEEQAAGVLSAPAKITNHLVALSEKASLNQPTDNHFNEALDIFYNIINGLHQENSNFDLASTKALIDAEFAQIDGLLAEIRQNGKVEDAVKATIDSRGEKLSIAMMKAWFEARGYEVTLINPVEKLLAHGGYLESSVDIEESTKRIDAPSIPKKNVVLMAGFTAGNEKGELVLLGRNGSDYSAACLAACLNASVCEIWTDVDGVYTCDPRLVPDARLLPSLSYREAMELSYFGAKVIHPRTIGPLVQPQIPCLIKNTGNPSAPGSIIDGNTTSEGLQVKGITNLDNLAMFNISGPGMQGMVGMASRVFSAMSKAGVSVILITQSSSEYSISFCVPVKAVDTAKTALEAEFEQELKAKALEPIDVIRDLSIISVVGDGMRQAKGIAARFFSALAQANISIVAIAQGSSERSISAVVAQNKAIEAVKATHQVLFNNKKVVDMFLVGVGGVGGELIEQVKNQKEYLAKKDIEIRVCALANSNKMLLDERGLDLTNWQEQLANATQPSDFDVLLSFIKLHHVVNPVFVDCTTAESVAGLYARALEEGFHVVTPNKKANTREMAYYNQVRANARKNQRKFLYDTNVGAGLPVIENLQNLLAAGDEVNRFNGILSGSLSFIFGKLEEGLSLSEVTALAREKGFTEPDPRDDLSGQDVARKLLILARETGLQIELDDVEVESVLPAGFAEGKSADEFMALLPQLDAEFATRVANAKAAGKVLRYVGQIENGHCKVSILEVDADNPLL",
    "response#2": "MRVLKFGGTSVANDQRVLNVAEIAESKLEDGQVALVLSAPAKITNHLVAMIEKTVEGKDVITHVNDAEQIFDTLLTGLRDKQPGFDYERLKKLTENEFAQIKQVLHGISLLGQCPDSINAAIICRGEKLSIAIMESVLTARGHKVTVIDPVKSLLAKGHYLESAVDISESTQRISALNIPADHIILMAGFTAGNEKNELVVLGRNGSDYSAAVLATCLRADCCEIWTDVDGVYTCDPRVVADARLLKGMSYQEAMELSYFGAKVLHPRTIAPIAQFQIPCLIKNTTNPSAPGTLIGDGQKDDSTPVKGITNLSNMAMINVSGPGMKGMVGMAARIFSVMSRKGISVVLITQSSSEYSISFCVPQNELKRARLALEEEFYLELKDGLLDELDVMEQLSIISVVGDGMRTLKGISARFFAALTRGNINIVAIAQGSSERSISAVIANEAGTTAVRLCHQMLFNTAQVVEVFIVGVGGVGSALIEQIHRQQQWLRQKHIDLRVCGIANSKAMLTDMQGIDLSHCASALAEASEPFSLSRLIRLEREYHFLNPVIVDCTSNQAIAEQYVNFLKDGFNVVTPNKKANTLSMDYYHQLRVAAEHSKRKFLYDTNVGAGLPVIENLQNLLNAGDELVHFSGILSGSLSFIFGKLDEGMSLSEATLLAKEKGFTEPDPRDDLSGMDVARKLLILAREAGYKLELDDINIEPVLPADFDSSGDVAEFMARLPSLDQQFADKLRQAAVDEKVLRYVGMIEEGRCQVKMVAVDGNDPS",
    "response#3": "MRVLKFGGTSVANDQRVLNVAEIAESKLEDGQVALVLSAPAKITNHLVAMIEKTVEGKDVITHVNDAEQIFDTLLTGLRDKQPGFDYERLKKLTENEFAQIKQVLHGISLLGQCPDSINAAIICRGEKLSIAIMESVLTARGHKVTVIDPVKSLLAKGHYLESAVDISESTQRISALNIPADHIILMAGFTAGNEKNELVVLGRNGSDYSAAVLATCLRADCCEIWTDVDGVYTCDPRVVADARLLKGMSYQEAMELSYFGAKVLHPRTIAPIAQFQIPCLIKNTTNPSAPGTLIISGPGMQGMVGMASRVFSAMSKAGVSVILITQSSSEYSISFCVPVKAVDTAKTALEAEFEQELKAKALEPIDVIRDLSIISLSGAGLIGVPGTAERMFAALRQAGVSVIMISQGSSEHSICCVVRQAQAEAGRAAVVEAFAEAIGDGQAQGVNAVVGSKIYKKYNIASKIFSALGSSKINVLAIAQGSSKHSISLVIKKEHILRSVQSVHNILFCNKKIIHVFLIGIGGIGVAGPGMKGMVGMASRVFEVMSNANISISLITQSSSEYSISFCIHNKDAARALNLLEDAFALELTNHLLDPIEVRLVVGSKIYKKYNIASKIFSALGSSKINVLAIAQGSSKHSISLVIKKEHILRSVQSVHNILFCNKKIIHVFLIGIGGIGVAGPGMKGMVGMASRVFEVMSNANISISLITQSSSEYSISFCIHNKDAARALNLLEDAFALELTNHLLDPIEVRLVVGSKIYKKYNIASKIFSALGSSKINVLAIAQGSSKHSISLVIKKEHILRSVQSVHNILFCNKKIIHVFLIGIGGIG"
  },
  {
    "reference": "MNFTHINEQGYARMVDVSSKNDSERIAIAQAIVKMQPETMGKIRDGAIKKGDVLGVAQIAGIMGAKQTSALIPMCHPLNLTSVNLEFAFDEVNASIIIEAEVKTTGKTGVEMEAITAVSVAALTIYDMSKAVDRWMEITDIKLLEKSGGKSGHLVRD",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein with cyclic pyranopterin monophosphate synthase activity functions is required to enable Mo-molybdopterin cofactor biosynthetic process.2. The protein should have a high affinity for substrate.3. We require a protein that can specifically bind to the metabolites produced in the molybdopterin biosynthesis, Cofactor biosynthesis pathway.4. We need a protein that can perform (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic   pyranopterin phosphate + diphosphate with high efficiency. The designed protein sequence is ",
    "response#1": "MVDISAKPDVVREAVAQGRIYLRPATLAAIREGSVVKGNVLATARVAATLAVKDTPRIIPMCHTIPLGAVTVDFTEGDGYIEATVVTKSTGKTGVEMEALTGVSVALLTIWDMVKSAEKDENGQYPVTCIEGIRVVEKKKIG",
    "response#2": "MVDVGDKAVTAREAVARGRVVMQPETLALIMDGKLPKGDVLAVARVAGIMAAKRTAELIPLCHLLNLSHASVQFTPDPASNALEIEATVRCQGQTGVEMEALTAVSIAALTIYDMCKAVDKTMQIDQIRLIAKRGG",
    "response#3": "MVDISAKPDVVREAVAQGRIYLRPATLAAIREGSVVKGNVLATARVAATLAVKDTPRIIPMCHTIPLGAVTVDFTEGDGYIEATVVTKSTGKTGVEMEALTGVSVALLTIWDMVKSAEKDENGQYPVTCIEGIRVVEKKKM"
  },
  {
    "reference": "MAQEGKAYIHLALAIYYAGGKIDEETLKKAAEAIGMQVDEAKIKMLVASLEEVNLEEVLKQAVAAPVAAAAAAPAAAPAAEEKAEEEKKEEEEEKKEEEVDLSGLSGMFGF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should prioritize the formation of helix elements.2. The protein should be optimized for stability and solubility when expressed in ribosome where it will be involved in translational elongation. The designed protein sequence is ",
    "response#1": "MPNIQKIRSFTLVSYVPNILNGRPLFARARGSACLVRYRNKLQHLGLIWVKLPSGKYRRVSNGNSVLLGKIAPKLRSELGNTRAGYWVNRGIKPQVRGIVKNPVDHPNGGRARSIARHRSPWGWR",
    "response#2": "IQKIRSFTLVSYVPNILNGRPLFARARGSACLVRYRNKLQHLGLIWVKLPSGKYRRVSNGNSVLLGKIAPKLRSELGNTRAGYWVNRGIKPQVRGIVKNPVDHPNGGRARSIARHRSPWK",
    "response#3": "NIQKIRSFTLVSYVPNILNGRPLFARARGSACLVRYRNKLQHLGLIWVKLPSGKYRRVSNGNSVLLGKIAPKLRSELGNTRAGYWVNRGIKPQVRGIVKNPVDHPNGGRARSIARHRSPWGWR"
  },
  {
    "reference": "MGTRLFFYVALCLLWAGHRDAEITQSPRHKITETGRQVTLACHQTWNHNNMFWYRQDLGHGLRLIHYSYGVHDTNKGEVSDGYSVSRSNTEDLPLTLESAASSQTSVYFCASSE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that In turn ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome; Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70; The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation; Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity.2. The presence of Ig-like domains is critical for the interaction of the designed protein with its intended targets.3. Incorporate a signal peptide in the protein design.4. The protein should be optimized for stability and expression in T cell receptor complex, plasma membrane where it will participate in adaptive immune response. The designed protein sequence is ",
    "response#1": "DKVVQSPLSLVVHEGDTVTLNCSYEVTNFRSLLWYKQEKKAPTFLFMLTSSGIEKKSGRLSSILDKKELFSILNITATQTGDSAIYLCAVP",
    "response#2": "PGIAQKITQTQPGMFVQEKEAVTLDCTYDTSDPSYGLFWYKQPSSGEMIFLIYQGSYDQQNATEGRYSLNFQKARKSANLVISASQLGDSAMYFCAMRR",
    "response#3": "TQPGMFVQEKEAVTLDCTYDTSDPSYGLFWYKQPSSGEMIFLIYQGSYDQQNATEGRYSLNFQKARKSANLVISASQLGDSAMYFCAVQTVTQSQPEMSVQEAETVTLSCTYDTSESDYYLFWYKQPPSRQMILVIRQEAYKQQNATENRFSVNFQKAAKSFSLKISDSQLGDAAMYFCAYRAQPSTVASSEGAVVEIFCNHSVSNAYNFFWYLHFPGCAPRLLVKGSKPSQQGRYNMTYERFSSSLLILQVREADAAVYYCAAAGVTQFPSHSVIEKGQTVTLRCDPISGHDNLYWYRRVMGKEIKFLLHFVKESKQDESGMPNNRFLAERTGGTYSTLKVQPAELEDSGVYFCASSSA"
  },
  {
    "reference": "MHNFKAIILAGGSGTRMKSRLPKPLHKLSGTPMIIWIVNAIIQSRIKDIITIVPANHEAFQNILEHKSELAVQDPPKGSGHALLQSKRKADKAKNILVLNSDMPLIKSETLTTLINHHQSSEADITVLTSKVKRPKGFGRIIRNSEGKITNIVEEPEADIEQKLIDEINAGAYCFKTSWLWDALESLKPSKSGEFFLTDLINFAHILKLNIESLITEEEEIIGVNNKSDLARAGKLSRAAICEKLMNDGVIIIDPDTTYIDSEVTIGADTTIFPNTHLTGNTNISFHSEIGPNSVISNSSIGSKCIIKSSTIESAVILDEVKVGPYSHIREGSILKSNVEIGNYVEIKNCTLGNGTKSGHHCYLGDSDIGSNVNIGAGTITCNYDGTDKHRTIIKDEAFIGSDTKLIAPVIIGEKSITGAGSIITKDIPDFSKGIGTPARILKGDRSL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.2. The designed protein must have at least one Nucleotidyl transferase domain.3. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc); The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain..4. The designed protein should have magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity to facilitate lipid A biosynthetic process.5. Ideal protein should have a binding affinity for key enzymes in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway to enhance the overall efficiency of the pathway.6. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "MAAVILAAGQGTRMKSNLYKVLHPVCGKPMVQHVVDQVSKVGVEETVVVVGHGAEKVQDQLGDKITYVLQEEQLGTGHAVVQAEELLKDKNGVTIVLCGDTPLITSETMEALLAYHEEQGAKATVLTAKATDPTGYGRIVRDDSGAVERIVEHKDATQEEREITEINTGTYCFDNTLLFSALKHVGNSNVQGEYYLPDVIEILQKNNDLIAAYYLPDPLETIGVNDRIALAEAEKTMQARRRELFMRHGVTLLDPARFDLRGELIHGRDVVLDANVIIEGKVELGDRVRIGAGCVIKNSVIGDDCEISPYSVVEDAVLEQKCTIGPFARLRPGAELAEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGAGVNIGAGTITCNYDGANKHKTIIGDDVFVGSDTQLVAPVSVASGATIGAGTTVTRDIAQNELVLSRVPQDMKVEGWGVTIINPASCTISEDAQIGLDVILEANSHIRGESKIASNCIIGPNTFIEDTFVDKNCKIINSTIFNSLIMEDINIGPYSHIRPGTKISSNSRIGNFVEIKNSHIEQSVKINHLSYIGDSEIGELTNIGAGTITANYDGTTKNRTKIGKNSKIGANSVFIAPVTLEENVTTGAGSVVNKNVEKXSXAIARVKGVPARVIK",
    "response#2": "MTSKTPKVLHLMLEEPILYYPLRSAQDAGFGDIAVMVGFEGELVESWTRDNFPGAEIIWQREQRGTGHAAKLAQEWWQNFENVMVLAGDAPLIKPETLSFFAERHAAGGNACSFLSFDLEDPTGYGRVLREDGKVRVVEHKDATERQREVKEVNSGMYIFDTAALAGQETAGALADGVTIIDPANTYIDNEVVIGADTVILPGCSLGGNSTIGADCTIGPNTEILDSQIGERVQIKQSVLSDAVVGNECTIGPFAYLRPGAKLGERVKIGDFVEIKNATLGNDTKVSHLSYVGDATIGKNVNFGCGAITVNYDGFNKQRTEVGDNAFVGSNSNLVAPVKIGKNAYIVAGSTITDEVMDEDMAIARQRQTVRGWADRQAALRTAVERKHQRTLILAAGAGTRMKSDTPKVLHTLAGRSMLAHSLHAVTKVAPRHLVVVAGRDRERVAPAALDIGRDLGRDVQIAVQEEQLGTGHAVVCGLSALPEDFTGVVVVTAGDVPLLDAATLAELVATHSAEAALVTVLTTTLPDPTGYGRVLRTQDGDVIGIVEQADATPSQQAITEVNAGVYAFDIAALRSALSRLSADNAQHELYLTDVIAIVRADGHLVRAHHVDEESVLLGANDRAQLAQVNAQLNRRVVRRWMDEGVTLLDPARFDLRGELIHGRDVVLDANVIIEGKVELGDRVRIGAGCVIKNSVIGDDCEISPYSVVEDAVLEQKCTIGPFARLRPGAELAEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGAGVNIGAGTITCNYDGANKHKTIIGDDVFVGSDTQLVAPVSVASGATIGAGTTVTRDIAQNELVLSRVP",
    "response#3": "MSSKTPKVLHLMLEEPILYYPLRSAQDAGFGDIAVMVGFEGELVESWTRDNFPGAEIIWQREQRGTGHAAKLAQEWWQNFENVMVLAGDAPLIKPETLSFFAERHAAGGNACSFLSFDLEDPTGYGRVLREDGKVRVVEHKDATERQREVKEVNSGMYIFDTAALAGSKTPKVLHLMLEEPILYYPLRSAQDAGFGDIAVMVGFEGELVESWTRDNFPGAEIIWQREQRGTGHAAKLAQEWWQNFENVMVLAGDAPLIKPETLSFFAERHAAGGNACSFLSFDLEDPTGYGRVLREDGKVRVVEHKDATERQREVKEVNSGMYIFDTAALAGHFVAGIEGVTIINPASCTISEDAQIGLDVILEANSHIRGESKIASNCIIGPNTFIEDTFVDKNCKIINSTIFNSLIMEDINIGPYSHIRPGTKISSNSRIGNFVEIKNSHIEQSVKINHLSYIGDSEIGELTNIGAGTITANYDGTTKNRTKIGKNSKIGANSVFIAPVTLEENVTTGAGSVVNKNVEKXSXAIARVKGWMTTKRERLAAKTAAE"
  },
  {
    "reference": "MRIVLATSNQGKIKEFKELMPHDEVCAFEELLGKMEIEETGTTFKENAILKAKTVYDKLNDNHAIVISDDSGISVQALNNEPGVYSARYAGINANDKANNAKLMAKLNEANLSSTPAFYTACIAIVYQGEVYTVHGWMHGEVIDHERGSNGFGYDPMFIPNGYTQTLGELENEIKKTFSHRSQALQLAKKVLDVLIKEH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.2. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.3. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate binding.4. The designed protein must be able to perform XTP diphosphatase activity, ribonucleoside triphosphate phosphatase activity under specific conditions. The designed protein sequence is ",
    "response#1": "GFTTAS",
    "response#2": "GFATKL",
    "response#3": "MNLRR"
  },
  {
    "reference": "MKVVTGEEMKAVDRYAIEKIGIDGRILMENAGRVAAKNIMKNFGRQKVCVLIGSGNNGGDGFVIAKVLQENDYDVSVCVIPERDEIKGDAEYHMNIYLNSGFSFTQYDQDVAESADIIVDTMLGTGIKGEIRSPYKEIFGELDKMDNTIISVDLPSGVPADESPVPEHALQADYTLIIGLPKLSYYTYPARKYYGEAKVIHIGLPKKAIDETVQSNIMLWSHEDTKNHWPARVDDSHKGSHGKVGIIAGSAGMPGAAVLTTSAAVRSGAGLTQLNTHGNIFGSVVNHVPEATLFDRKDDLESFIEGKDVIAIGPGLGVNGETSRTVEYLVNEYEGTLVIDADGLTRLEQLKMQIKKRQAPVVISPHPGEMARLIDSSSGEVNKNRFRISREIAMEYGMHVVLKGPNTLVATPEGEIFINDTGNAGLAKGGSGDVLTGMIAAFLGRYDQPQQAISSAVYMHGFTADYISEAGIGVETITASDLIDNLRHTFHLIRN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The (6S)-NADPHX, K(+) binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.3. The protein design must be able to perform ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, ATP binding, NADHX epimerase activity, metal ion binding in order to contribute to nicotinamide nucleotide metabolic process.4. The protein requires Mg(2+) to activate a critical catalytic site.5. The protein should have a fast turnover rate for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "LLVIGGSDKYHGAPILSAMSSIRAGVDLVIISTPELIANTIRAHTPNFIVRDYPGRYLTLDSVKYISDLINWADAIVLGPGLGVESETKEAVCKLFTLIKEANKPLVVDADALKVLAENPRILAGSPAVATPHRGEFKLLTGLEIKSSADILDNINEILSKISEFDITFAVKGAVDLVAYKNMFKLDLLGNPGMTVGGIGDVLTGVLGCFLSQGVEPFKAACAAVFITGLSGNLIAEKKGYHFTSAELIEYITDDIVRKLLPLRKAYAHKGDAGRAVICAGSPGFTGAAALSSYAAVKAGAGLVALYTPLSSRDVLAGKLTEVMVHGLLERMPGILGGGATGDVVKNANAADVLAIGPGLGTSESTQQVVRDILLQVETPVVIDADALTALQGHTEILTQMQAAKVLTPHPGEMARLIGKEISEINADRINIAAQYAQEWNAVLVLKGAPTVIACPDGTVYVNSTGSSALATGGSGDVLTGIIAGLAAQEITLQEAAVCGVYLHGKAADISGIDIGLASGELAQFLPEARCEVLLKRRFFENRKGDFGKLLVIGGSDKYHGAPILSAMSSIRAGVDLVIISTPELIANTIRAHTPNFIVRDYPGRYLTLDSVKYISDLINWADAIVLGPGLGVESETKEAVCKLFTLIKEANKPLVVDADALKVLAENPRILAGSPAVATPHRGEFKLLTGLEIKSSADILDNINEILSKISEFDITFAVKGAVDLVAYKNMFKLDLLGNPGMTVGGIGDVLTGVLGCFLSQGVEPFKAACAAVFITGLSGNLIAEKKGYHFTSAELIEYIPEA",
    "response#2": "MREIDNHTIEVLGIPGIVLMENAARGVCEVIYDRVKGSSALVVCGKGNNGGDGLAVARNLYNMGYDVEVILTAPVEELKGDAKKNAEILSALPVPVHVVDSTEKLFELYTFAKECDFIVDGLFGTGLSKPLSGFYAELVDFINRLNKTVISIDIPSGLSADTGQVIGPHVKADYTVTFAFPKVAHVMPPACYSVGEVFVVDISIPEDVPEAVSRWLPVRRPGAHKGNCGHVVVIGGAPGYSGAGRLAGMAALRAGAGRVTLVTHPEHAALANLERPELMVRPAESARDLQTLLAGADAVAVGPGLGQQAWGRALWPAVADWTGPLVVDADALNLLAQAPRRRDDWVLTPHPGEAARLLAVTTGEVTADRVAAVDAIRRRFGGVVLLKGAGTLVAPAPGGGMACITGGHPGMASAGMGDVLTGVMAALRAQGLGPGEAAAAGAAWHVAAARLAARRIGAERGMLAGDVIDALPGE",
    "response#3": "MAMADAAAIAGGVPGADLMEAAGRAVAEAVLERHLPRPVVVLCGPGNNGGDGFVAARHLHAAGWPVRVALLGDRSALHGDAASASQAWKGPVEALSLGLLDGHPLVIDALFGAGLGRPIDGIAGELIDRMNAEALHVVSVDIPSGLHGDSGEVMGCAPMAECTVTFFHAKPGHYSVEGLRRCGSLRVVDIGIDAVPDTAEAVLVIGGDVGFAGAPLLAGEAAARVGCGLVTLATRPQHAGMQVAARPELMAWGVDRPAQLDPLLDRASVVAIGPGLGQSPWAAGLLARVLETNLPLVVDADALTLLARDPVQNERWVLTPHPGEAARLLDCSTAEIQGDRFTAVRELQSRYGGAIVLKGCGSLVATRKSGVTVCTDGNPGLASGGTGDVLTGVIAGLMAQGLEPESAALTGACLHGAAADRAVLEGERGMLAGDLEPADYADVVAPHTNNVDKYSRGSVLVVAGSSRYPGAAVMAAKAAARAGAGYVTLAVPVTIAPLIQMQLPEVPVVGVAAEQDGTFSAAARPVILKLAERSSATLVGPGMRVTGGTVAVVSALINSKAPLVVDADGLNCLARLTNGHLDEFPEVTRRDAPLVLTPHRRELGRLVGLADTPPDSLTAALEAARRIVWADGGSELCIVAKGTATACVGVEAALLPEPGTASLATAGSGDVLGGIICALLARMPDDIEDLPLIAAFGCEVHAVAGSRAAKRYGSRGVMAGDIADEVGLAVDELGG"
  },
  {
    "reference": "MLKPLENRVVLRVKEEEEKSMGGIVLTSASQEKPQTAEVIAVGEGKTTNHGTLISPLVKVGDTVIFEKFSGTTVKMDGEEFLILKDSDLLAIVE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit ATP-dependent protein folding chaperone.2. The protein must exhibit the following characteristics: Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The designed protein sequence is ",
    "response#1": "ETTVG",
    "response#2": "MPETR",
    "response#3": "MDIRPLHDRVIIKRQEVESKSAGGIVL"
  },
  {
    "reference": "MTTVPKNAVAKTLYQKVWDAHVVATPEGEAPIIYVDRHLVHEVTSPQAFSGLKVAGRKLRAPEKTFATMDHNTSTRSASLDALSPMARTQVETLAQNCKDFGVRLYDIHHPNQGIVHVMGPELGITFPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLRQLKAKTMKIEVRGHVSDGVTAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMVAPDQTTFDYLAGREFAPKGEDWAEAVAYWQAIKTDDGAVFDAVVELDAADIAPQLTWGTNPGQVVAIDGKVPNPLDEANPSTRASMEKALEYIGLSAGTPMTDISINKVFIGSCTNSRIEDLRSAAVHAKGRKVASGVTAIVVPGSGQVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNDDRLEAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. We require a protein that can specifically bind to the metabolites produced in the L-leucine from 3-methyl-2-oxobutanoate: step 2/4 pathway.2. The protein design should incorporate a binding pocket that specifically recognizes [4Fe-4S] cluster to enhance enzymatic efficiency.3. The protein must exhibit the following characteristics: Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate.. The designed protein sequence is ",
    "response#1": "RHLVHEVTSPQAFEGLREAGRKVWRVSSIVATADHNTPTTGWERGYDGIADPISKEQIVTLDKNIQESGAAAFFPFLSKRQGIVHVIGPENGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQTLLAKKAKNMLVKVNGKVAPGITAKDIVLAIIGKIGTAGGTGYTIEFAGEAIRDLSMEGRMTVCNMAIEAGARAGLVAVDDKTINYVKGRPLAPTGVEWDQAVAYWKTLHSDADAKFDAVVELNAAEIVPQVTWGTSPEMVLGIDAVVPDPDKEKDPSKRGAIERALTYMGLQPGKPMDDIFVDKVFIGSCTNSRIEDMREAAAVVKKLGQKVAKNIKLAMVVPGSGLVKEQAEREGLDAIFKAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIHGHG",
    "response#2": "RHLVHEVTSPQAFEGLRNSNRKVRQPGLTLAVADHNVPTTDRSKGIADAESKIQVDTLESNCKEFGVQYLGMNDKRQGIVHIIGPEQGFTQPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLVQKKAKNFRINVNGKLPIGVTSKDVILQIIGQIGTAGGTGSVIEYAGSLISSLSVEQRMTICNMTIEGGARAGLIAPDEKIFEYLKGKPMSPKNENWDKAMKYWESLKTDDGAKFDKEINLVAEDILPMITWGTSPQDVITIDGKVPNPKNEQDEDKKNSLERSLNYMGLKADTLATDIKIDKVFIGSCTNGRIEDLREAAKILKDKKKASHVQAMVVPGSGLVKEQAEQEGLDKIFIASGFEWREPGCSMCLAMNADKLKPGERCASTSNRNFEGRQGRGGRTHLVSPGMAAAAAISGHFVDIR",
    "response#3": "LERSNVDTDLIIPKQFLKTIKRTGLGSALFWPLRYDAKTGEPDPAFVLNQKPYDQSKILVVTGPNFGCGSSREHAAWSLLDFGIRAVIAESFGDIFRNNLTKNGQLPVVLSRAQIQRLTQDAKAGKQITVDE"
  },
  {
    "reference": "MRAIGLMSGTSMDGIDAALIETDGETVAWLGSALTIPYGAETRALLEGAMADARGVNARTDRPGRLVEAERRITTLHGAAVRALLDSLRLTPADIDVVGFHGQTVLHRPDAALTVQIGLGQALADDLRIPVVADLRAADVAAGGQGAPLVPVFHQALVRGLDLPQPVAVLNIGGVANVTVLEDGADPIACDTGPGNALLDDFMAARTGRPYDDDGASAARGQVDEAAVAEVLGHPFFAAPPPKSLDRNEFRAFVTERLHLAGASTDDGAATLTALSAASIAAVVPLLPKAPKSWIVAGGGARNSTLRAMLAERLGVPVVVAEQVGWSADALEAHAFGFLAVRSLKGLALTFPTTTGAPEPLTGGVLFQPTP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The ATP binding site should be located in a region of the protein that is accessible to the ligand.2. We are looking for a protein with broad substrate specificity for 1,6-anhydro-N-acetyl-beta-muramate + ATP + H2O = ADP + H(+) +   N-acetyl-D-muramate 6-phosphate.3. The protein design must be able to perform ATP binding, kinase activity in order to contribute to phosphorylation, amino sugar metabolic process.4. For general function, the protein need meet that Is required for the utilization of anhMurNAc either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling.; Catalyzes the specific phosphorylation of 1,6-anhydro-N- acetylmuramic acid (anhMurNAc) with the simultaneous cleavage of the 1,6-anhydro ring, generating MurNAc-6-P5. The protein design should consider the endogenous proteins and enzymes present in the Cell wall biogenesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MITITGSNGKSTVTTLVAEALKEAGYAVGVGGNIGCPALDLLTHPTEFDVYVLELSSFQLETTYSLQTIAATVLNISEDHMDRYLALEDYIQAKMTILNNTELAVLPLDFERVGIARPGDEVRFGLNYAEALPPKEYGIVMKNGQAWLGWEDHASVPVTAMAQQGLHHQLNALAMMEFFQPKLMAITGSNGKSTVTTLVAEALKEAGYAVGVGGNIGCPALDLLTHPTEFDVYVLELSSFQLETTYSLQTIAATVLNISEDHMDRYLALEDYIQAKMTILNNTELAVLPLDFERVGIARPGDEVRFGLNYAEALPPKEYGIVMKNGQAWLGWEDHASVPVTAMAQQGLHHQLNALAMMAGMQLGLSLSDVLAALPAFSGVPHRLEFFYEKGGLRFYNDSASTVPEATIAALEAFDESVVLIVGGTDKNADYQPLAQAAAKAHALYLLAGSATARLQPLLHAAGDVYRLGEELLAHL",
    "response#2": "MITGSNGKSTVTTLVAEALKEAGYAVGVGGNIGCPALDLLTHPTEFDVYVLELSSFQLETTYSLQTIAATVLNISEDHMDRYLALEDYIQAKMTILNNTELAVLPLDFERVGIARPGDEVRFGLNYAEALPPKEYGIVMKNGQAWLGWEDHASVPVTAMAQQGLHHQLNALAMMEFFQPKVVVFGEKDYQQLALIRKMSSALNLPIAIVGVPTRRDSDGLALSSRNYLSETERRKAAGIAKAMQQGAEQIRQGNRDIASLLTAAETELSALSGLTVEYAEIVEGDSLQPVEKIAGDRLMLLAARVGSTRLIDNIPL",
    "response#3": "MITGSNGKSTVTTLVAEALKEAGYAVGVGGNIGCPALDLLTHPTEFDVYVLELSSFQLETTYSLQTIAATVLNISEDHMDRYLALEDYIQAKMTILNNTELAVLPLDFERVGIARPGDEVRFGLNYAEALPPKEYGIVMKNGQAWLGWEDHASVPVTAMAQQGLHHQLNALAMMEFFRPKVITGSNGKSTVTTLVAEALKEAGYAVGVGGNIGCPALDLLTHPTEFDVYVLELSSFQLETTYSLQTIAATVLNISEDHMDRYLALEDYIQAKMTILNNTELAVLPLDFERVGIARPGDEVRFGLNYAEALPPKEYGIVMKNGQAWLGWEDHASVPVTAMAQQGLHHQLNALAMMGAELAGLSPNKILEAIASFSGVQHRLEFFYEKGGLRFYNDSASTVPEATIAALEAFDESVVLIVGGTDKNADYQPLAQAAAKAHALYLLAGSATARLQPLLHAAGNIEEALASYANRHFGGEK"
  },
  {
    "reference": "MKLGIVILAAGQGTRMKSSLPKVLHSLAGRPLLAHVVETALSLQPEKTVVVYGHGGEKVREAFDHPELFWVEQSEQLGTGHAVQQAMPMLQEMDRILVLYGDVPLIRRESLQSLIDNSGSAVGLMTVNLDDPSGYGRIQRDREGTVQCIVEHKDASEQQLTVCEINTGIMLVDAGRLHHWLQELSPKNAQGEYYLTDIIAMAVRDGIDIHVTQPGDIVEAEGVNDRLQLASLERVLQRWQAEALMHDGVTLMDPARFDLRGSLITGTDVTLDVNVIIEGQVELGNGVSVGANTVLRNVRVADNVRIRENCVIEEAVIGAESIIGPFSRIRPGTELIGGVHVGNFVEIKNSTIGLGSKINHLSYIGDTRMGGGVNIGAGTITCNYDGAYKHQTIIEDHAFIGSNAALVAPVCVGKKATIGAGSVIVKDAPADELTVSRSKQLTIKGWRRPKKET",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a high specificity for acetyl-CoA binding, with minimal off-target effects.2. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.3. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.4. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.5. The protein should be optimized for activity in UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway and exhibit minimal cross-reactivity with other metabolic pathways.6. A protein that contributes to lipid A biosynthetic process by functioning in cytoplasm with magnesium ion binding. The designed protein sequence is ",
    "response#1": "VVVLAAGEGKRMKSALPKVLHPLLGRTLVGHVLAAAAPLAAERTLVVVGHGADQVAAHLSEVAPQATPVRQTEQLGTGHAVRIALDAVADLAGTVVVLNGDVPLLRAETVTALVETHEAAGAAATVLAAEVADPTGLGRMVRDGERLLKIVEEKDASAAERAITLCNSGVMAFDARLALDLLGRVGNANAKGEYYLTDLVGLARADGLGCAVVEAAEDELLGVNTRAELAAAEAAYQARARARAMADGVTLIAPETVFFSHDTQLGRDVVVEPHVVFGPGVVVEDNVTIHSFSHIEGATIHTRAEIGPYARLRPGADIGVKAKVGNFVEIKKASLGEGAKANHLSYIGDAIVGANANIGAGTITCNYDGFFKYRTDIGAGAFIGSNSALVAPVTIGAGAIVGAGSVVTRDVGADALALVRPEQRHIEGWAAAFRAKRAASK",
    "response#2": "MAGVVLAAGMGTRMKSRLPKVLHRVCGKEMVRYPVELLRRLGADRTVVVVSPANRAEIERALGDQVEYVEQASPNGTGGAVAESAKLLQAKLLQKKDVQNPEHLLVLGSDSPLLKEETLRQLMAHHLEDGEPALAMLTVRTDDPTGYGRVLREGGHVVGIVEEKDADAAQRAVDEVNAGVYAFDAGPLREALGRLSTDNAQGELYLTDVLGIARADGQRVGAAVAADHRELLGVNDRAQLSELRALANRRLLDAWMRAGVTLLDPMTTWIDVTVTLDRDAVVDQNTQLRGATTVGAAAAVGPDVTLIDTTVGAGAVVLRSHAVGAEIGPQASVGPYAYLRPAARLADKAKVGTFVEVKNSEIGPGAKVPHLSYVGDATIGARANIGAATIFVNYDGVAKHHTTVGEAAFVGCDTNLIAPVEIGPGAYVAAGSAITQDVPPGALGVTRASQRNIERWVRERRPGSAAAQAAAAALEGQE",
    "response#3": "MSAIVLAAGQGTRMKSSLTKVLHPVCGKPMVQHVIDQLSSLQLDEIITVVGRSADEVRNQIGTKSQFVVQEEQLGTGHAVQQAEPILGTNKGTTLVVAADTPLLTAETLESVFQYHEQEQAKVTVLTAHAEDPSGYGRVIRDEQGDVARIVEHKDASPKEQEVQEINTAIYCFDNEALFESLQNVSNDNAQGEYYLPDVIEILKNNGEKIAAYQSYQVQDPVEAMGINNRVQLAEVEKVMRARINRRLMLSGVSMLDPDTTYIANDAVFEEDITLYPGVIIEKGSRIGKGCVITSGSIIKNSEIGKNCYIQNSVITDSVVGNNVKIGPYAHLRPKSRLSDNVKIGNFVEIKNSSFGENSKSSHLTYIGDAEIGENVNLGCGVVFVNYDGKNKYLTIVEDDCFVGCNVNLVAPVRVKKGAYIAAGSTITDDVEEYSLAIARSRQVNKPGWAEKNRDSK"
  },
  {
    "reference": "MTSVLMIDNCDSFTYNLVDQFSPHGTIVIVKRNHPFYDGEIEAIMALTSICITPGPCYPAEAALNSCSIIGHLAGRIPILGICLGQQALGQARGGLVIFAHGKLSNIEHNGIFAPLFNPPRALPAGRYHSLVVEPARIEVTGQCNQLEVVPQEIMAIRHRDLPVEGVQFHPESILSSNGAAILANLIHRPCH",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be optimized for activity in Cofactor biosynthesis, 4- aminobenzoate from chorismate: step 1/2 pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The designed protein must have a active site that is structurally stable and resistant to denaturation.3. The protein contains novel Glutamine amidotransferase type-1 domains that confer a unique function or activity.4. The protein must exhibit the following characteristics: PabA converts glutamine into glutamate only in the presence of stoichiometric amounts of PabB .; Part of a heterodimeric complex that catalyzes the two-step biosynthesis of 4-amino-4-deoxychorismate (ADC), a precursor of p- aminobenzoate (PABA) and tetrahydrofolate; In the first step, a glutamine amidotransferase (PabA) generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by aminodeoxychorismate synthase (PabB) to produce ADC. The designed protein sequence is ",
    "response#1": "MFITGGVVSSLGKGIASASIAALLEARQLKVALLKLDPYINVDAGTMNPFQHGEVYVTDDGTETDLDLGHYERFVRIQVSQKNNFTTGRIYENVIRRERRGDYLGATVQVIPHITDEIKRCCKEICDDTDMTQVVMVEIGGTVGDIESLPFLEAIRQLGGEVGHERALFVHLTLVPYIPTVGELKTKPTQHSVKELRSIGIQPDILLCRSSYPLPENERRKIALFTDVNEQAVISLVDADSIYRVPKLLHQQGVDQIICDKLKLVDSYKSLSEAVAHASIHTRTQVEILYIDAEDIETTGTDCLNGVNAILVPGGFGNRGINGILQAVQFARENNVPYLGICLGMQLAVIDFARHIIGLVGAHSTEFDPHTSFPVIALIAEWTTTEGRVERRSQQSDLGGTMRLGGQICHLMPGTQTYQLYGKDIVRERHRHRYEFNNRYISMFRQAGLVFAGMSADGKLVEVIENIRHPWFIGCQFHPEFTSTPRDGHPLFTGFIKESYKSLTESFVHVGAHLDTRVNLKWIDSESIDEKCEGNLFCDVDGILVAGGFGQRGVEGKIEAIKYAREHKIPFLGICLGMQLSLVEFARNVLHLEDADSVEFNPECKNPIIYLIDEFIDASGNKQLRTHKSPLGGTMRLGEYACDTKAGSLLRKTYGGAKTIHERHRHRYEANPAYRAAFEKEGLIVSGESDGLIEVVELKEHPWFLGVQFHPEFTSRLKRPNADFAAAARVVLVGCGTAAYAAMTASYSIEAWAGLPVSVELSHEFRYREPVLNSKTLAVFISQSGETMDSLMAVRYARQAGVKTISVCNVMDSSIPKESHAVIYTKAGPEVAVASTKSFVCQIV",
    "response#2": "MFITGGVVSSLGKGIASASIAALLEARQLKVALLKLDPYINVDAGTMNPFQHGEVYVTDDGTETDLDLGHYERFVRIQVSQKNNFTTGRIYENVIRRERRGDYLGATVQVIPHITDEIKRCCKEICDDTDMTQVVMVEIGGTVGDIESLPFLEAIRQLGGEVGHERALFVHLTLVPYIPTVGELKTKPTQHSVKELRSIGIQPDILLCRSSYPLPENERRKIALFTDVNEQAVISLVDADSIYRVPKLLHQQGVDQIICDKLKLVDSYKSLSEAVAHASIHTRTQVEILYIDAEDIETTGTDCLNGVNAILVPGGFGNRGINGILQAVQFARENNVPYLGICLGMQLAVIDFARHIIGLVGAHSTEFDPHTSFPVIALIAEWTTTEGRVERRSQQSDLGGTMRLGGQICHLMPGTQTYQLYGKDIVRERHRHRYEFNNRYISMFRQAGLVFAGMSADGKLVEVIENIRHPWFIGCQFHPEFTSTPRDGHPLFTGFIDAYADGVDRLKDYLGSGDMYQVNLAQRRVGMISAEDYQLYIRLRDANPAPYMAYLDIDEGLLVASPERIILDEASDLDTRPIAGTLRGRPRAGGDDEDDGRAIDLLRVDKDRAERIMIVDLDRNDIARVGVGGSVKVREIMGLERYSGVMHLVSQVTGDLQEAIEAVDLIRAGFPGGTLTGAPKVRTMEIIDELEPQRRAAYCGSIGYIAYKGNIDFKIAIPTLYALAGQLFCQAGGGVVGDSVPDGEYRESFEDYQRVVESAKAAFRRGDLFEVVPGQTFAEPCADAPSSVFRRLRAANPAPYEAFVNLGRGEFLVAASPEMYVRVAGGRVETCPISGTVARGADALGDAAQVLRLLTSAKDAAELTMCTDVDRNDKARVCEPGSVRVIGRRMIELYSRLIHTVDHVEGRLRSGMDALDAFLTHSWAVTVTGAPKRWAMQFLEDTEQSPRRWYGGAFGRLGFDGGMDTGLTLRTIRMAEGVAYVRAGATLLSDSDPDAEDAECRL",
    "response#3": "MFITGGVVSSLGKGIASASIAALLEARQLKVALLKLDPYINVDAGTMNPFQHGEVYVTDDGTETDLDLGHYERFVRIQVSQKNNFTTGRIYENVIRRERRGDYLGATVQVIPHITDEIKRCCKEICDDTDMTQVVMVEIGGTVGDIESLPFLEAIRQLGGEVGHERALFVHLTLVPYIPTVGELKTKPTQHSVKELRSIGIQPDILLCRSSYPLPENERRKIALFTDVNEQAVISLVDADSIYRVPKLLHQQGVDQIICDKLKLPIPKADLSEWEKVVYNEDNPKESYKSLTESFVHVGAHLDTRVNLKWIDSESIDEKCEGNLFCDVDGILVAGGFGQRGVEGKIEAIKYAREHKIPFLGICLGMQLSLVEFARNVLHLEDADSVEFNPECKNPIIYLIDEFIDASGNKQLRTHKSPLGGTMRLGEYACDTKAGSLLRKTYGGAKTIHERHRHRYEANPAYRAAFEKEGLIVSGESDGLIEVVELKEHPWFLGVQFHPEFTSRLKRPNAEVDAACGQLKAKLVDSYKSLSEAVAHASIHTRTQVEILYIDAEDIETTGTDCLNGVNAILVPGGFGNRGINGILQAVQFARENNVPYLGICLGMQLAVIDFARHIIGLVGAHSTEFDPHTSFPVIALIAEWTTTEGRVERRSQQSDLGGTMRLGGQICHLMPGTQTYQLYGKDIVRERHRHRYEFNNRYISMFRQAGLVFAGMSADGKLVEVIENIRHPWFIGCQFHPEFTSTPRDGHPLFTGFIKSKKL"
  },
  {
    "reference": "MKFIVKLQAEITIKSRPVRKRFTKILESNIKNVLRRVDEQVTTRMNWDNVEVNTKDNSPENRAKLVEALKCIPGVPMFLEVQQTEFTDLHDIFEKTLAVHSKTIENKTFCVRVKRTGSHEFNSLKIEQYVGGGLNQQVENAKVQLRKPDVTIRLEVKNKDLFIVTERHQGLGGFPIATQEDVLSLMSGGFDSGVASYQMIKKGSRTHYCFFNLGGSAHEVGVKQVSYYLWNKFGASHRVKFFAVDFEPVVAEILEKIENGQMGVVLKRMMVRAATKVAEKANIQALVTGESLGQVSSQTLTNLNVINRVTDTLILRPLAAYDKQDIIDIARNIGTEDFAKTIPEYCGVISKKPTVKAVLSKVEAEEDNFDFSILDKVVDETRVLDIRDIGKETEAEVKAVELVEEITHDAVVVDIRSPEEEDENPLDIENVDVIHIPFYKLATRFGDLDMSKEYLLYCDQGVMSKLQALYLQDNGFSNVKVYRP",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor.2. The Cysteine persulfide intermediate of the protein must be engineered to enable effective catalysis of [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 +   S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier   protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-   cysteinyl-[cysteine desulfurase].3. A protein should be able to manipulate the activity of enzymes in the Cofactor biosynthesis pathway, either by activating or inhibiting their function.4. The designed protein must have at least one Rhodanese domain.5. The designed protein should be involved in thiamine diphosphate biosynthetic process by localizing to cytoplasm and exhibiting CCA tRNA nucleotidyltransferase activity, ATP binding. The designed protein sequence is ",
    "response#1": "MGSQGEVLSLVSGGFDSTVASYLMMRRGLKTHYIFFNLGGAAHEIGVKQVAYYLWEQFGASHRVSFVSVPFEPVVAEIFRTSHETYMGVVLKRLMLKAAEKIADQMGIDALVMGDAVGQVSSQTLRNMAMIDRVTDKLVLRPLSVMHKQDIMNLADQIGTREFAESMPEYCGVISQNPVINGTQEDVLSLISGGFDSGVSSYLHIKRGSKTHYCFFNLGGPAHEIGVKQVAHYLWNKFGSSAKVKFIAVDFEPVVAEILEKVDDGQMGVVLKRMFMRAGGMIAEKFGIQALVTGEALGQVSSQTLTNLRHIDVVTDTLILRPLINWDKEDIIDLSRIIGTEDFAKTMPEYCGVISRKPTVKAVKAKLEAEEEKFKHVKRDDKFSRVDNYPLTTLDDIRDLAMASWQERLAGKTFCVRVRRGGTHEFSSMDVERHVGAGLFMAGNTAGVKMRDPDVVVPIEIRGEHVFLVQDDANDEVPVDAIIIDIRRDEEQEEAPLDVPGHKVLHIPFFRLASKQDMLDKSKSYLLYCEQGVMSRLQAIHLQEQGFSQVAVYEEK",
    "response#2": "IVKPHPEIFVKSESVRKRFTKILECNIRNIIQRRTESVAVFNRRDHIEVTSESDQYFNEVIEILTTTPGIHHVLEVKQSDFTDMHDIFEQVLELNRPNIENKTFVVRAKRRGKHDFTSIELERYVGGGLNQAVESASVKLHKPDVTVKIEIAGDKLNQVIARHKGLGGFPLGTQEDVLSLISGGFDSGVSSYLHIKRGSKTHYCFFNLGGPAHEIGVKQVAHYLWNKFGSSAKVKFIAVDFEPVVAEILEKVDDGQMGVVLKRMFMRAGGMIAEKFGIQALVTGEALGQVSSQTLTNLRHIDVVTDTLILRPLINWDKEDIIDLSRIIGTEDFAKTMPEYCGVISRKPTVKAVKAKLEAEEEKFDYSVLEKVEEGSVVELVEEALVGQVVLDIRASDEQDARPLHLSGIEVVSMPFYTLGSRFAALDPARAYLLYCEKGIMSQLHAQYLLDAGHDNIRVLQK",
    "response#3": "IVRLHAEITIKSKSVRQRHLKVLTGNIRTLLKPLHDSMRVRNHWDRIEVHHDDLPELTALTIRHLGQIPGIAYFEQVVEHPLTDFETLLEWILPLQTDRLQNKSFAVRVKRKGKHEFSSMDMAIYLGGGIRARVPGTHVQLKNPQEEIVIHVIEQQVSLVQQRFAGLGGFPLPTQETVVSLMSGGFDSAVASFQMIRRGARTHFCFFNLGADAHEAAVREISYFLWEKYSKTHAVKFVAVDFSEVVERILEAGDQGSLGVLLKRAMMRASGLVAERLNAQAIVTGEAVGQVSSQTLSNLKLIDQATDTLILRPLIVQDKQTIVDCAREIGVETLSAAVPEYCGVISKQPSVKVKPEVIAASEQAALPGQVVLDIRHPDLAEDEPLELAGIEVETVPFYALNNRFKELDGNRQYLLYCDKGVMSRLHAHHLLSEGHQNVRVYRPASV"
  },
  {
    "reference": "MSEVVISGIGSAFSGSSNIQDFGRDLLANKHLVQRTKRWTDGVFTPVCGQTPYEYFDDLFFGMSRRLALVSDPTTKISCMRSFEAILDAGLHPATLFGSKTGVFAASGINEFEQTALYTIDNARLQDGYIIMGMSRSMLANRLSYYFDFKGPSCAVDGSWVGGLLGLKQAADAIREGRCDTALVVAGSISRYGETSLLLNQLGLVTDEGVTRSFDANGTGAVRSDGAVALLLQRADQAKRCYARVLATEVEFVGPGCDLNPTLPSPKGTQDFLRRIYEKHAIDPKTIAYLEADGNGNRIRDACELNAIDQALVQKAARASPLQIGSVKSNIGHVDCVNGLAAICKMVIAMETGVIPATINFQEPNSEATGLVAGRLKVVDKNTPLILSDDSVLAVHTIALSSMMGHTVLGGNPRGPGCVQDPADHLPRLITFSARNEEAAADVAKKIQSAPFDTNYYRLLQDVFSDDIRGYTYRGFVICPSSGDDTLIAPLEKRTVWFVYSGMGSQWAGMGSALMKLPVFAETIERLHAVLQPRGVDLKAILTETGPTAFDNILKSFVGITACQVALTNVLTAVGVVPDGIVGHSVGELGCAYADGCLTEEQAILAAFARGKASSAVPLIKGKMAAIGLGYQDVLPRLPPTIDVACHNSSTSCTLSGPAQDVDAFVEKLSAEGVFARGVNVSDLAYHSRYIQPAAPHLLQHLKEVIPEPKPRSSRWITTSVPVDKRDSELAKFCSAEYQTNNLLSPVLFEEALPYIPEQAVLIEVAPH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The presence of Enoyl reductase (ER) domains is critical for the interaction of the designed protein with its intended targets.2. The protein design must be able to perform enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity, 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity in order to contribute to fatty acid biosynthetic process.3. The protein have enhanced dodecanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxotetradecanoyl-   [ACP] + CO2 + holo-[ACP]. The designed protein sequence is ",
    "response#1": "AGSRHRGPSLYGLPRRNGKLKEIDRFDAGFFGVHPKQAHTMDPQLRLMLEISYEAIVDGGINPVAMRGTKTGVYIGVSGSEAGEAFSKDPEELLGYSMTGCQRAMLANRLSYYFDFNGPSTAIDTACSSSLLALENAFNAIRHGQCDAALVGGVNLLLKPNTSVQFMKLGMLSPDGTCKSFDSSGNGYCRSEAAVAVLLTKKSMAKRVYATVLNAGNNTDGYKEQGVTFPSGHMQQRLVRSLYQEVNVSPEQVEYIEAHGTGTKVGDPQEVNGIVSVFCQSQREPLLIGSTKSNMGHPEPASGLAALAKVVLSLEHGMWAPNIHFHEPNPDIPALRDGRVSVVVEPVPVRGGIVGINSFGFGGSNVHVILRPPGHTNLAPSCLCYSGMGSQWCKMAVDLMELPIFASAMYECHEILKPKGIDLMKIVTEDDPKIFDNILHSFVGIAAIQIGLTDTLKALGIVPDGIIGHSVGELGCAYADGCFTKEQMIMCAYSRGKASLDATLITGMMAAIGKGYQQMKDECPPTIEVACHNGPDSCTLSGPKEDMEKYVKELQDRNIFARLVNVSNIAYHSRYIKPAAPLLLENLKKVIPEPKARSSKWISTSVPEDKWDTDLAKTCSAEYQTNNLLSSVLFEEGCRHIPKDAIVIEIAPHGL",
    "response#2": "AGSRHRGPSLYGLPRRNGKLKEIDRFDAGFFGVHPKQAHTMDPQLRLMLEISYEAIVDGGINPVAMRGTKTGVYIGVSGSEAGEAFSKDPEELLGYSMTGCQRAMLANRLSYYFDFNGPSTAIDTACSSSLLALENAFNAIRHGQCDAALVGGVNLLLKPNTSVQFMKLGMLSPDGTCKSFDSSGNGYCRSEAAVAVLLTKKSMAKRVYATVLNAGNNTDGYKEQGVTFPSGHMQQRLVRSLYQEVNVSPEQVEYIEAHGTGTKVGDPQEVNGIVSVFCQSQREPLLIGSTKSNMGHPEPASGLAALAKVVLSLEHGMWAPNIHFHEPNPDIPALRDGRVSVVVEPVPVRGGIVGINSFGFGGSNVHVILRPPGS",
    "response#3": "AGSRHRGPSLYGLPRRNGKLKEIDRFDAGFFGVHPKQAHTMDPQLRLMLEISYEAIVDGGINPVAMRGTKTGVYIGVSGSEAGEAFSKDPEELLGYSMTGCQRAMLANRLSYYFDFNGPSTAIDTACSSSLLALENAFNAIRHGQCDAALVGGVNLLLKPNTSVQFMKLGMLSPDGTCKSFDSSGNGYCRSEAAVAVLLTKKSMAKRVYATVLNAGNNTDGYKEQGVTFPSGHMQQRLVRSLYQEVNVSPEQVEYIEAHGTGTKVGDPQEVNGIVSVFCQSQREPLLIGSTKSNMGHPEPASGLAALAKVVLSLEHGMWAPNIHFHEPNPDIPALRDGRVSVVVEPVPVRGGIVGINSFGFGGSNVHVILRPPGRSATAGWYRTVATDAIARELGADFSFRNADAFADTTKTETLDLLAERFGGVDYLIYSVAAPRRTDPRSGTTYQSVLKPLGAPHTTRNLEFADDGAAQVREVTVAPATEAEAAATVGVMGGEDWSRWITALAERGLLRSGFRTVALTYIGSPLTSAIYRGGTIGAAKAHLESTARALTERLAAVDGRAFTSVNGALVTQALTAIPGIPLYVSLLRGVLGDRFPSPVAQSLDLWPPPSPRSATAGWYRTVATDAIARELGADFSFRNADAFADTTKTETLDLLAERFGGVDYLIYSVAAPRRTDPRSGTTYQSVLKPLGAPHTTRNLEFADDGAAQVREVTVAPATEAEAAATVGVMGGEDWSRWITALAERGLLRSGFRTVALTYIGSPLTSAIYRGGTIGAAKAHLESTARALTERLAAVDGRAFTSVNGALVTQALTAIPGIPLYVSLLRGVLGDRFPSPVAQSLDLW"
  },
  {
    "reference": "MASKTVLFNKHLESKAKMVDFHGWEMPINYGSQIEEHHAVRQDAGMFDVSHMTVVDVTGSDACAFLRKLLANDVAKLTVPGKALYGGMLDENAGIIDDLITYYLTDTHYRVVVNSATRDKDLAWINKQAAAFDVVVTERPELAMIAVQGPNAKAKAATVFSAEQNAAVEGMKPFFGVQSASLFIATTGYTGEAGYEIIVPETEAEALWQALLDAGVKPCGLGARDTLRLEAGMNLYGQDMDESINPLAANMGWTVAWEPSERDFIGRDALTAIKAAGTDKLVGLVMEEKGVLRHDMAVFFTDADGVEHQGVITSGSFSPTLGYSIAMARVPSGIGAIAEVEMRKKRVNVKVIAPSFVRNGKQAF",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: The glycine cleavage system catalyzes the degradation of glycine..2. The designed protein should have transaminase activity, aminomethyltransferase activity to facilitate glycine decarboxylation via glycine cleavage system. The designed protein sequence is ",
    "response#1": "LHPKHLEAGAKMVDFHGWEMPINYGSQLEEHHAVRTDAGMFDVSHMTIVDLTGERVKAFLQHLLANDVAKLTVPGKALYSGMLNPDGGVIDDLITYYLTDTFYRLVVNSATREKDLAWIRHHAIDFAVSVTERPELAMIAVQGPNAKAKAAKVFTPEQNAAVEGMKPFFGVQAGDLFIATTGYTGEDGYEIVVPQEKACDLWQALLDNGVAPCGLGARDTLRLEAGMNLYSQDMDESISPLAANMAWVSEAVRRHIGPDSSQQQEMLNYVGAESLDDLTAQIVPESIRLSQELSIGDSCGEAEGIAYIRGLAKQNQVFKSYIGMGYYGTQVPNVILRNVLENPGWYTAYTPYQPEIAQGRLEAILNFQQVSMDLTGLDLASASLLDEATAAAEAMALAKRVSKAKKANIFFVADDVFPQTLDVVKTRAECFGFEVVVGPASEAVNHELFGALFQYSNRFGQITDFTDLFAELRAKNVIVTVAADIMSLVLLKSPGSMGADVVFGSAQRFGVPMGFGGPHAAFFVARDEHKRSMPGRIIGVSKDTRGNRALRMAMQTREQHIRREKANSNICTAQILLANMASFYAVFHGPQGLKTIASRINRFADILAAGLQAKGVSLVNNTWFDTISIKGLDVAAVNARALAAEMNLRFDADGIVGVSLDETTIRTDIDALFERHIGPRAADTQAMLQRLGYDSLDTLIGNVIPDSIKGSSVLDLPAGMGEAEALASLKAIAARNRALRSFIGQGYYNCHTPAPILRNLLENPAWYTAYTPYQPEISQGRLEALLNFQTLVSDLSGLPIANASMLDEATAAAEAMTFCKRLSKNRTSQAFFASRHCHPQTLDVLRTRAEPLGIEVVVGDESTIEDFSAYFGALLQYPTCDGEIVDYRELVSRFHAVDALVAVAADLLALTLLTPPGEFGADVAIGSAQRFGVPLGFGGPHAAYFATRDAFKRDMPGRLVGVSIDRHGKPAYRLAMQTREQHIRREKATSNICTAQVLLANIASMFAVYHGPQGLLRIARRTHRLTAILAAGLERLGVAVEQKHFFDTLSLATGARTAAIHAKARAAGINLREIDAGRLGLSLDETVRQTDVETLWG",
    "response#2": "LHPKHLEAGAKMVDFHGWEMPINYGSQLEEHHAVRTDAGMFDVSHMTIVDLTGERVKAFLQHLLANDVAKLTVPGKALYSGMLNPDGGVIDDLITYYLTDTFYRLVVNSATREKDLAWIRHHAIDFAVSVTERPELAMIAVQGPNAKAKAAKVFTPEQNAAVEGMKPFFGVQAGDLFIATTGYTGEDGYEIVVPQEKACDLWQALLDNGVAPCGLGARDTLRLEAGMNLYSQDMDESISPLAANMAWVSEA",
    "response#3": "LNAIHRSLGARMVDFGGWDMPVNYGSQIEEHHAVRRDAGIFDVSHMCVVDLTGARVRDFLRGLLANNIDKLQTPGKALYTCMLNPKGGVIDDLIVYFFREDWFRLVVNAGTAPTDIEWITAQNAAAGTGVAITPRRSDNNAGAEPLGIVAVQGPNARAKAYAALPGTQAVGEALKPFNAGFATIDGVGEIMVARTGYTGEDGFELVVPAAQIAGVWERLLQAGVRPCGLGARDTLRLEAGMNLYGQDMDEHVSPLDAGLAWVEKSAYMG"
  },
  {
    "reference": "MVLLNGKLKYIAVVAIFYNLIILLVKEKFPYICTKKKFHAISNRILYEYLNNFVSKDIFRREDITLKNLNFVQTNLKSDKDAEIKENRDTQSVDDNMFQRVYKFILNFFYGNKKNRINKSMNYGKYDNFNKINDIFEFMRNNGLPINITSVCLIDTGLNIKDALINYFLNHDISTYNSYTYHSVNINYKKPDSFNFGINSENCDEDNYSECESTFLENHNGHGKYEDKSTIQGDSLKLIEKKYDKNVDLQRSGIDVEICKAFNNSKEKKNSLNIIPVIKCLEYCKTKNVKIIHMDYNINEQNEQLIQIMDDLKNSEIFVILPSEKLFNEKPYEDNSVIYPSSFFEKFENVFFIGSLDYSDMSSDDADIASNFQIQKNEYLKYRKNNVFLLDSINSSLKKRDDHDILYYEIKYSSAFFINIITIILNIYPNMSIKELRNILSYSIPSKETAQLKTENIFEGNFDINKFIHILLNRGINSSGFVRKYKDVTPNESTNKIFLLEDQKDADIEPQKDSSIDIYCDEGGSDEDIVSTSNGLDVYSEYSHSNNKSDQLLNDEKGLKYETYKDLYSVKENDIYVFESNNPTNSSFMQMNYDDKIKSKYLDNLEEANYQNHRTQFEINRNDNRYPIISEDNLRDRQNMHNIQMLNDGINYSENANNIEELYDNDFEDYRGKDLNPEYNKIRDNNNNKNINKEFGILNTWRKNNEGLYLSDSEEESQPFKWMDMHVDDIYQDRKKRLKGNNYDNRNGRVRRIYEDNKRGRYIKNRNM",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have a specific function within the plasma membrane. The designed protein sequence is ",
    "response#1": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPQ",
    "response#2": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPV",
    "response#3": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPQ"
  },
  {
    "reference": "MPNRSQSSEPGVAVNARPANPIETAITALAELSGARLDWLHAEWSGAGKAATVVSGVDLRAQDIVAGDLFAALPGARSHGAQFAGDALASGAAAILTDDDGLALLRASNPSVDRPVMLLVHPKPRDVLGEISAEIYSRPSEKMQVIGITGTSGKTTTSYLLEAALVGAGRNPGLVGTIETRIHGRRVPSALTTPEAPHLHALFAVMAEQGIDTVVMEVSSHALSLGRVDGIRFDIGAFTNLSQDHLDFHRDFEEYFAAKARLFDPASTVHADSAVICVDDGWGRRMAEVASAGDVTTVATTEAESDWKVGSVEVAADGSQLFDLSGPNEQSWPVSLRLPGRYNVANAALALTVSAKAGIDVQSAVAAVGSVDVPGRVQRIDRGQDFLAVVDYAHKPAALEAVIATLRNQVSGRVGVVVGAGGDRDAGKRALMGEAGARGSDLLIVTDDNPRTEVPATIRAAVMAGALAVPESERGVVLEVGDRAEAIEQAVRWAQPGDVVLVAGKGHETGQEIDGVKHPFDDREVLGDAIDRIAIDTAHGGTK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The ATP, meso-2,6-diaminoheptanedioate, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding site should be stable and able to withstand changes in environmental conditions.2. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..3. The designed protein have Mur ligase C-terminal, Mur ligase N-terminal catalytic, Mur ligase central domains with specific sequence motifs that are crucial for its activity.4. The protein should be designed to have high stability and activity towards ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.5. The protein's functional design necessitates the inclusion of a Meso-diaminopimelate recognition motif motif.6. We require a protein that can specifically bind to the metabolites produced in the peptidoglycan biosynthesis, Cell wall biogenesis pathway. The designed protein sequence is ",
    "response#1": "MQEGWLFAALPGTQAHGASYAGTALARGASAILTDADGARLAAAHLAGTDVAVVVAEDPRATLSRTASAWIYGQPSDRLSVVGVTGTNGKTTTTYLLESIFVEAGLPSGVIGTTGVRILGRTVPFERTTPEAPDLQRLLRRMADEGVVTVAMEVSSHGLDQHRVDGTRYSCAVFTNLSQDHLDYHPSMQEYFDAKARLFTPELSERAVVNADVPEGRALARRSLVRTRTFGLEPGAEIRASDVEVTHSGISFSVDGLAVRSRLRGNFNVSNCLGALAAALDTGVPAAVAAAGLAETVVPGRVERFKATDDQPLIVVDYAHTPDALDQVLQALRPHVAGKLWCVFGCGGDRDRGKRPQMGAIAERLADRVILTDDNPRHEAGDAIVREIAGGMRSSARIIRDRREAIATALSEADAADVVLVAGKGHEDYQILGTEKVHFDDREAAAAALAAREVGI",
    "response#2": "MVDKITSLECDSRKIKRGSLFVAVRGYNVDGHKFIPLVTLAGAAAIVCEELPERLESTVTYIKVPDSAVALGLLASQFYGRPSEKLSVLGVTGTNGKTTIASLLYQLFKKAGFKVGLLSTVRIMVDNKAYKATHTTPDSLTINKYLKEMNDKGVAYCFMEVSSHGIHQHRTEGLHFQGGIFTNLSHDHLDYHDTFAEYRDVKKTFFDQLPATAFALVNMDDKNGLIMLQNTKAKKYTYALKGYADYRAQILENQLNGLLLKINESEVWTRLIGNFNAYNVLAIYAAALQLGLEAEEILRVLPSLNAVPGRMQHAATRENGATVFVDYAHTPDAVATALAALRPHVLGRIVAIVGAGGDRDRGKRPRMGAAAAEHADVVIVTDDNPRSEDPAAIRRAVLDGAPGAMEVGDRAEAILRGVDMLEPGDALLICGKGHETYQILKDKTIHFDEREVVRELLETIG",
    "response#3": "MKLRDLFPEIKGIEISGLSSDSRKTKPGDLFAALTGSQSDGRAYVDQAVAAGAVAVLAEPGLPPCGVPVIEVPHARAALSRAASRFYREPSQRLHLIGTVTGTSGKTSVAAFTRQIWQTAGNAAASIGTTGVRSPTRDDYGTLTTPDPVDLALLLRELADEGVTHAAMEASSHGLTQRRLDGVRLAAGGFTNLGRDHMDYHPTVEHYFESKMRLFDTLLPKTAPAVVFADDQWSAAAIDAASRAGLNVHTVGRNGNLLKLKRLEHERHRQIGEIEAEGRLFRIVLPLAGEFQMANALVAAGLAVASGADPARVIAAVAGLTGVPGRVERFKATDDQPLIVVDYAHTPDALDQVLQALRPHVAGKLWCVFGCGGDRDRGKRPQMGAIAERLADRVILTDDNPRHEAGDAIVREIAGGMRSSARIIRDRREAIATALSEADAADVVLVAGKGHETYQIIGTEKRHFDDREEARRAIAAHQS"
  },
  {
    "reference": "MTKKYNVAICGATGVVGRKMIQVLEERNFPVNQLYLFASKRSAGKTLTYKGEAVTIQELTEDSLKTPIDIALFSAGGETSLKYAPIAAKNGVFVIDNSSAWRMDDTVPLVVPEVNPQALNADSKIIANPNCSTIQSVVPLIALKPYGIKRIVYTTYQAVSGAGKEGISDLENHETNKFPYSINDNVLPHIDTFLENGYTKEEIKMIKETQKILEQPDLKITATCVRVPIHTSHAVSINIELEQSFTLDDIRQAFNNLDGVIVQDDPQHNLYPLQSKATDHDGVFVGRIRRDESVANGLNLWVVADNLRKGAATNTIQIAESLIEQKLI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design should take into account the physiological conditions of the Amino-acid biosynthesis pathway.2. A protein with NAD binding is needed to enhance isoleucine biosynthetic process, diaminopimelate biosynthetic process in a specific context.3. The protein should be able to bind substrate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "LGPLHEQYKIKRVIVSTYQSITGTGVKAVQQLENEYVGEKGEMAYPYPIHRNAIPHCDVFEANGYTKEEMKLVNETQKILDDRTIAVTATAIRIPVVGGHSESVNLEFEKEYTETDVRKILSDTSGVTVQDNPDTNTYPMPIYAEGKNDVFVGRIRRDHSRENALNMWIVADNLRH",
    "response#2": "LGPLHEQYKIKRVIVSTYQSITGTGVKAVQQLENEYVGEKGEMAYPYPIHRNAIPHCDVFEANGYTKEEMKLVNETQKILDDRTIAVTATAIRIPVVGGHSESVNLEFEKEYTETDVRKILSDTSGVTVQDNPDTNTYPMPIYAEGKNDVFVGRIRRDHSRENALNMWIVADNLRA",
    "response#3": "LGPLHEQYKIKRVIVSTYQSITGTGVKAVQQLENEYVGEKGEMAYPYPIHRNAIPHCDVFEANGYTKEEMKLVNETQKILDDRTIAVTATAIRIPVVGGHSESVNLEFEKEYTETDVRKILSDTSGVTVQDNPDTNTYPMPIYAEGKNDVFVGRIRRDHSRENALNMWIVADNLRG"
  },
  {
    "reference": "MTPEKYRPTITPDLPNRQWPNQRLTQAPIWASVDLRDGNQALANPMTVEQKLKLWDQLVAIGFKTIEIGFPAASQLEFDFARRLIEENRIPDDVTVQVLVQAREHLIKRTYEALQGVKQAVVHVYNTTSTVQREKVFCKSKADIKAMAIQGAKWVQSYAKEHPESDWVFQYSPESFSQTETDYAVEVCQAVMDVWQPTPDNRCILNLPATVESTSPNRFADQVEYFITHLKNRESAIISIHTHNDRGCAVAAAELSLLAGADRVEGTLLGNGERTGNMDIVTLAMNLYSEGIDPELNLSDPDSWVPVIEEVTKIDTHVRHPWVGEAVYTAYSGSHQDAIRKCLMRQKDDEPWNVAYLPIDPKDLNRSYEAIIRVNSQSGKAGAAFVLTQEYDLNLPKWVQQDFAPVAQTIAEQAGGIVSHQMLYDAFIQHYRLDKSLAELNNYQLSRKEGKEHLSVEVNGETWQGQGNGTLSALCDAWQRRTGQQVDVLDYSEHALHEGKDSKAIAYVYVQQKNTKTIGIAMAEDTVSAMIQALLSTIR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. We are looking for a protein with broad substrate specificity for 3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-   isopropylmalate + CoA + H(+).2. A protein should be able to manipulate the activity of enzymes in the L-leucine biosynthesis, L-leucine from 3-methyl-2-oxobutanoate: step 1/4 pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "IVGENAFGHEAGIHVHGVIENPLTYEPISPEEVGNFRRLALGKHSGIHGLKKLLEEQGIYLNDQELREVLNEIKKLAENGEI",
    "response#2": "LILPGDGIGPEVMAEVRKIIGWFGDKRGLNFDVSEDLVGGAAYDVHGVPLADETMAKAQEADAVLLGAVGGPKYDDLDFSVKPERGLLRLRKEMDLFSNLRPAQCFDALADFSSLKKDIVAGLDIMIVRELTSGVYFGEPRGIFEEGNERVGINTQRYTESEIERVARSAFELAMRRSKKLCSMEKANVMESGILWREVVTRVAKDYPEVELSHMYADNGAMQLVRAPKQFDVILTDNLFGDILSDCAAMLTGSLGMLPSASLGAPMANGRPKALYEPVHGSAPDIAGQGKANPIACILSFAMALRYSFDQGAEADRLEAAVEQVLADGVRTADLLGTEGVTPVSTTEMGAAIVG",
    "response#3": "VRIFDTTLRDGEQAPGIDLTVEQKVKIARKLAELGVDVIEAGFPAASEGEFIATKKILEEVGDQVEVIGLSRANKQDIDKTIDTGISSIHVFIATSDIHLKYKLKMTREQVLDKIYESVRYAKDHGLIVEYSPEDATRTDKDFLLKAVSTAIEAGADRINIPDTVGVMHPFKFHDLIKDVVSVTKDKIVSVHCHNDFGLATANSIAGVMAGARQVHVTVNGIGERAGNASLEEVVMALKKLLGYEVNIKTYKLYETSRLVSELTGVPVPYFKAIVGENAFGHEAGIHVHGVIENPLTYEPISPEEVGNFRRLALGKHSGIHGLKKLLEEQGIYLNDQELREVLNEIKKLAENGEKVNVDVAKELTSRYG"
  },
  {
    "reference": "MQHKRSRAMASPRSPFLFVLLALAVGGTANAHDDGLPAFRYSAELLGQLQLPSVALPLNDDLFLYGRDAEAFDLEAYLALNAPALRDKSEYLEHWSGYYSINPKVLLTLMVMQSGPLGAPDERALAAPLGRLSAKRGFDAQVRDVLQQLSRRYYGFEEYQLRQAAARKAVGEDGLNAASAALLGLLREGAKVSAVQGGNPLGAYAQTFQRLFGTPAAELLQPSNRVARQLQAKAALAPPSNLMQLPWRQGYSWQPNGAHSNTGSGYPYSSFDASYDWPRWGSATYSVVAAHAGTVRVLSRCQVRVTHPSGWATNYYHMDQIQVSNGQQVSADTKLGVYAGNINTALCEGGSSTGPHLHFSLLYNGAFVSLQGASFGPYRINVGTSNYDNDCRRYYFYNQSAGTTHCAFRPLYNPGLAL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that can perform metalloendopeptidase activity in extracellular space to support proteolysis, protein secretion by the type II secretion system.2. The protein must exhibit the following characteristics: While either His-317 or His-356 can abstract a proton in the hydrolysis reaction, the same residue performs both functions in a given catalytic cycle, with the other stabilizing the catalytic intermediate.; Enhances the elastolytic but not proteolytic activity of elastase (lasB) and elastolytic activity of other proteases; Degradation of host elastin is likely to contribute to the pathogenicity of P.aeruginosa.3. turn-strand-strand-strand-strand-strand-strand-strand-strand-helix-turn-strand-strand-strand-turn is the desired arrangement for the engineered protein.4. The Proton donor/acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.5. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "QRQAWKHHFITYRINNYTPDMRRQDVDDAIQKAFQVWSDVTPLKFRKINVGEADIMIFFAYGEHGDFNPFDGQDGVLAHAFGPGIGRGGDAHFDEAETWSTNFKGKNLFLVAVHEFGHSLGLGHSSDRNAIMFPNYGYFDPNTFSLSADDTRRIQSLYGTDPKSSVTPHANLTFKFLQSVSSIPSGDTGFVKFNAEQIEQAKLSLQSWSDVANLTFTEVTGNKSANITFGNYTRDASGNLDYGTQAYAYYPGNYQGAGSSWYNYNQSNIRNPGSEEYGRQTFTHEIGHALGLAHPGEYNAGEGDPSYNDAVYAEDSYQFSIMSYWGENETGADYNGHYGTDPKSSVTPNIDAAVFNPVTYKTYFFADDRYWRYDERRKLMDPGYPQLIIRHFSGVRD",
    "response#2": "SGDTGFVKFNAEQIEQAKLSLQSWSDVANLTFTEVTGNKSANITFGNYTRDASGNLDYGTQAYAYYPGNYQGAGSSWYNYNQSNIRNPGSEEYGRQTFTHEIGHALGLAHPGEYNAGEGDPSYNDAVYAEDSYQFSIMSYWGENETGADYNGHYGGAPMIDDIAAIQRLYT",
    "response#3": "WKHHFITYRINNYTPDMRRQDVDDAIQKAFQVWSDVTPLKFRKINVGEADIMIFFAYGEHGDFNPFDGQDGVLAHAFGPGIGRGGDAHFDEAETWSTNFKGKNLFLVAVHEFGHSLGLGHSSDRNAIMFPNYGYFDPNTFSLSADDTRRIQSLYG"
  },
  {
    "reference": "MTKFVFITGGVVSSIGKGIVAASLGRLLKSRDYSVSILKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTDTSMSRLNSVTTGSIYQSVINKERRGDYQGGTVQVIPHITHEIKERIHRVARNTNPDVVITEIGGTVGDIESLPYLEAIRQFRKDVGRNDVAYMHVTLIPWISAAGEMKTKPTQHSVKELRSIGIQPDVLVCRCDRPLEPGIKEKLSEFCDVPVSSVITARDAKSIYEVPLIMEGEGLAQQILELVQLEAREPELQQWRTLVERLYCPTRKIEVAIVGKYVQLSDAYLSLVEALRHAAIAAGSEINLRWVSAEDLEINGGEPYLKDVSGVIVPGGFGIRGVDGKVTAVEYARKHHIPFLGLCLGMHCAVVEWARHIAHIDNANSAEFDPDTDNPVINLLPEQQDVVDLGGTMRLGLYPCRLGARTRAFSLYQQEVIYERHRHRYEFNNAYRNLFLETGYAIAGTSPDGRLVEIIELPDHPFFIATQFHPEVRSRPNTPHPLFLGFVKAGLEKRAVDITASEAETELQFTAEVY",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that can modulate the overall flux of metabolites through the CTP from UDP: step 2/2, Pyrimidine metabolism, CTP biosynthesis via de novo pathway pathway, enhancing or suppressing its activity as needed.2. The presence of Glutamine amidotransferase domains is critical for the interaction of the designed protein with its intended targets.3. The designed protein must be optimized for ATP binding, CTP synthase activity.4. The protein should be designed to catalyze the following reaction H2O + L-glutamine = L-glutamate + NH4(+).5. The CTP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "VTIAFVGKYLDLKESYKSLTEALIHAGAHINAKINIHWCDSERIEDAGAYEVIQTCDAVLVAGGFGHRGVEGKLKAIEYARTNNIPYLGICLGMQLAVIEYARNVLGIEDANSVEFDPEAKNPLIYLIDEFLDQSGNKQVRTHESPMGGTMRLGEYPFTPKKGSKLQKAYGDKETYYERHRHRYEANPKYKEALEKAGMIISGESHGLIEAVELKDHKWFVGVQFHPEFTSHLETPNPIILEFIRQAVEQ",
    "response#2": "LQDAYISVNEALRHAGYPVNAKVEITHLNSEDVNEQNVDELLQDAEGILVPGGFGTRGIEGKITAIKYAREHDVPYLGICLGMQMACVEFARDVVGLKDANSTEINPETPFNVIDLMPDQENVENLGGTQRLGSYPCQLKPATVAAAAYDGTELIHERHRHRYEFNNKFREQLADHGMVFSGTSPDNRLVEVIELPKQKFFVAAQYHPEFLSRPNRPEGLFRAFIDSYKSLNEALYHGGFAHRLKVNIKWIEAEALEEPGGVALLEGADAILVPGGFGDRGTRGMMKAAQVGREREIPYFGICYGFQWATVEYARNVAGLTDADSTECTPDTPTKVIYKLRDLLGVDELGGTMRLGSYACELAPGSLAQKVYGTHLIHERHRHRYEFNCLYERPLAQHGLKITGRSPDGKFAEIAELPGHPWYLAVQFHPEFRSKPTRPHPLFASFVDAAIARKDAYKSLGEALRHGGIKQNTAVHLKWLDSEQIEAEGAAAVLGDVDAVLVPGGFGKRGFEGKVQAAQYAREHKVPYFGICYGMHAAVVDFARHVAGIEGADSSENDRHAKDPVIALVTEWTTASGEVETRSEQSDLGGTMRLGAQEARLKPGTLAHNLYGRDVVRERHRHRYEFNNRYRQQFEDLGMVVSGKSMDDLLVELVELPPQTHPWFLACQAHPEFTSTPRDGHPLFIGFVKG",
    "response#3": "VTVAIVGKYVEHKDAYKSLGEALRHGGIKQNTAVHLKWLDSEQIEAEGAAAVLGDVDAVLVPGGFGKRGFEGKVQAAQYAREHKVPYFGICYGMHAAVVDFARHVAGIEGADSSENDRHAKDPVIALVTEWTTASGEVETRSEQSDLGGTMRLGAQEARLKPGTLAHNLYGRDVVRERHRHRYEFNNRYRQQFEDLGMVVSGKSMDDLLVELVELPPQTHPWFLACQAHPEFTSTPRDGHPLFIGFP"
  },
  {
    "reference": "MSSVPAPREYFLDSIRAWLMLLGIPFHISLIYSTHSWHVNSATPSWWLTLFNDFIHAFRMQVFFVISGYFSYMLFLRYPLKRWWKVRVERVGIPMLTAIPLLTLPQFILLQYVKEKTENWPTLSAYEKYNTLAWELISHLWFLLVLVILTTVSIGIFTWFQKRQETSKPRPAAISLARLSLIFFLLGMAYAAIRRIIFIVYPAILSDGMFNFIVMQTLFYVPFFILGALAFIHPDLKARFTTPSRGCTLGAAVAFIAYLLNQRYGSGDAWMYETESVITMVMGLWMVNVVFSLGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHISSNLIGFLCGLIFVMGIALILYEIHLRIPLLKFLFSGKPPVKQESRAAIG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Could catalyze the transfer of succinyl residues from the cytoplasmic side of the membrane to the nascent glucan backbones on the periplasmic side of the membrane.; Necessary for the succinyl substitution of periplasmic glucans.2. The ideal protein would be one that plays a critical role in glucan biosynthetic process through acyltransferase activity, transferring groups other than amino-acyl groups, transferase activity, transferring one-carbon groups within plasma membrane.3. The protein should be optimized for activity in Glycan metabolism, osmoregulated periplasmic glucan (OPG) biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "LLVLDADSLMESSTITGLAQRMQADPDAGLIQTIPSLINGTTLMARLQQFAARIYGPVIGTGLGWWVQKEGNFWGHNAIIRTEAFMTAAGLPNLKGKPPFGGHIMSHDFVEAALIRRAGWSVVIAYDLPGSYEECPPSIIDLAVRDRRWCQGNLQHSRILPTKGLHWVSRLHLLTGIMAYLSSPFWLMLILTGL",
    "response#2": "LLVLDADSLMESSTITGLAQRMQADPDAGLIQTIPSLINGTTLMARLQQFAARIYGPVIGTGLGWWVQKEGNFWGHNAIIRTEAFMTAAGLPNLKGKPPFGGHIMSHDFVEAALIRRAGWSVVIAYDLPGSYEECPPSIIDLAVRDRRWCQGNLQHSRILPTKGLHWVSRLHLLTGIMAYLSSPFWLMLILTGLLVLDADSLMESSTITGLAQRMQADPDAGLIQTIPSLINGTTLMARLQQFAARIYGPVIGTGLGWWVQKEGNFWGHNAIIRTEAFMTAAGLPNLKGKPPFGGHIMSHDFVEAALIRRAGWSVVIAYDLPGSYEECPPSIIDLAVRDRRWCQGNLQHSRILPTKGLHWVSRLHLLTGIMAYLSSPFWLMLILTGLL",
    "response#3": "LLVLDADSLMESSTITGLAQRMQADPDAGLIQTIPSLINGTTLMARLQQFAARIYGPVIGTGLGWWVQKEGNFWGHNAIIRTEAFMTAAGLPNLKGKPPFGGHIMSHDFVEAALIRRAGWSVVIAYDLPGSYEECPPSIIDLAVRDRRWCQGNLQHSRILPTKGLHWVSRLHLLTGIMAYLSSPFWLMLILTGG"
  },
  {
    "reference": "MKIAKNVVVSIAYQVRTEDGVLVDEAPVNQPLEYLQGHNNLVIGLENALEGKAVGDKFEVRVKPEEAYGEYNENMVQRVPKDVFQGVDELVVGMRFIADTDIGPLPVVITEVAENDVVVDGNHMLAGQELLFSVEVVATREATLEEIAHGHIHQEGGCCGGHHHDSDEEGHGCGCGSHHHHEHEHHAHDGCCGNGGCKH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The ideal protein would be one that plays a critical role in protein refolding through metal ion binding, peptidyl-prolyl cis-trans isomerase activity within cytoplasm.2. The protein contains novel PPIase FKBP-type domains that confer a unique function or activity.3. The protein should be designed to have high stability and activity towards [protein]-peptidylproline (omega=180) = [protein]-   peptidylproline (omega=0).4. The designed protein should have a binding site that is specific to the target Ni(2+) ligand.5. The protein must exhibit the following characteristics: This function in hydrogenase biosynthesis requires chaperone activity and the presence of the metal-binding domain, but not PPIase activity .; Also involved in hydrogenase metallocenter assembly, probably by participating in the nickel insertion step. The designed protein sequence is ",
    "response#1": "MGASLKKIEKSVATLELTIPKEKFEEGLDYAFKKNASKFNVPGFRKGKAPRFLVERYYGEGVLYEDAIEYVFHEAYQEALKTFNLEPVDYPDINILQIGKGKDLVLEATVAVMPEVELGEYKGIEIEKIEYDVYDGDVEYELEKYRDKQAELDQNTVASLAYQLTIEDGVVVDQSTVDAPLDYLHGHNNLITGLERELEGKVAGDKFTVTIAPEDAYGEHNEDLVQRVPADVFQGVDELEVGMIEKNTVASLAYQLTIEDGVVVDQSTVDAPLDYLHGHNNLITGLERELEGKVAGDKFTVTIAPEDAYGEHNLSLEDLKVKTKEEIKSRKIQAASDAKETEAVQAAVDNAEIAEIPEAMLNEDVDRQVNQYLANMQQQGISPEMYFQLTGSSEDQLRDQLREGAENRVKTTLVLEAIVEAEKIDPSDDEVAAEIKDLAEQYGMEEKAVRSALSEDMIKHDVAIKSAVELIDAADVKIPEINEEILSLEDLKVKTKEEIKSRKIQAASDAKETEAVQAAVDNAEIAEIPEAMLNEDVDRQVNQYLANMQQQGISPEMYFQLTGSSEDQLRDQLREGAENRVKTTLVLEAIVEAEKIDPSDDEVAAEIKDLAEQYGMEEKAVRSALSEDMIKHDVAIKSAVELIEHSKATNKTVSREELFADED",
    "response#2": "MGASLKKIEKSVATLELTIPKEKFEEGLDYAFKKNASKFNVPGFRKGKAPRFLVERYYGEGVLYEDAIEYVFHEAYQEALKTFNLEPVDYPDINILQIGKGKDLVLEATVAVMPEVELGEYKGIEIEKIEYDVYDGDVEYELEKLQQAEAERKAEEAAKAGNTVAMRYIGKLEDGKVFDANKKGKPFTFKLGKGEVIKGWDIGIAGMAVGGERRITIPPHLAYGKKALPGIPANSKLIFDVKLVGVREPELPEVDDEFAKDVNELDQLRQVVREQLSAEFARAMRQHVKRALFDRLDETHKFDAPPTLVEEEFEQVWKTIMAEMEKEKKTFADENTTEEEARAEYRRIADRRVRLSLVLSEIGEKNGITVADDEINRALISRARQTPGREKEIWDYYQRNPQALAQIRAPLFENKVVDFILENATKVSEAIEKNTVASLAYQLTIEDGVVVDQSTVDAPLDYLHGHNNLITGLERELEGKVAGDKFTVTIAPEDAYGEHNG",
    "response#3": "MGASLKKIEKSVATLELTIPKEKFEEGLDYAFKKNASKFNVPGFRKGKAPRFLVERYYGEGVLYEDAIEYVFHEAYQEALKTFNLEPVDYPDINILQIGKGKDLVLEATVAVMPEVELGEYKGIEIEKIEYDVYDGDVEYELEKLEKKRGGANHVKAENGDRITISFKGTIDGELFEGGSSEDILVVLGSNALIPGFEEQLVGAGVGETRTVKASFPGNYADSELAGKDAEFEGKLTFVNDTLEELKADSRKRLETTKQYDQATQAQERVLEELLKLAEVPIPEKLLADEVQTRKHNLEHHQLGQMGLDLEKYLEIQGKTLEEFENETSEQAIKGIKTQFILDELVNKEKLNVNQEELTEHLMRRAASSGMSPDQFAQAVVEGGQVPMLVGEVARGKALAVVVIEKNTVASLAYQLTIEDGVVVDQSTVDAPLDYLHGHNNLITGLERELEGKVAGDKFTVTIAPEDAYGEHNVAAKAGNTVAMRYIGKLEDGKVFDANKKGKPFTFKLGKGEVIKGWDIGIAGMAVGGERRITIPPHLAYGKKALPGIPANSKLIFDVKLTELK"
  },
  {
    "reference": "MSAPLPAPLHVLHTPHALQCAGAALPWLRARVSGSLHSDSRLIAPGDGFIAWPGASTDGRAHVADAIARGATACLVEQAGVAAFAFPAAPGASLASLPGLKAAAGPIAAQWFDQPSRQLDVLAVTGTNGKTSTAWWLADALNGRAKSPVCALAGTLGLGVPPVLETAGMTTPDPLRLQRALRQFVDRGLSACAIEASSIGLEQGRLAGTQIRAAIFTNCTQDHLDYHGSMAAYWRAKRALFDWPGLQAAVINIDDPAGAQLHAELAGRVPDLWSISTQGPARLAARDITLAAQGLRCTVREGAAPQRLTTRVIGHYNLQNLLAVLATLRSLGWPLAEALQAGAGLQPVPGRMQQLALAGQPLVVVDYAHTPDALAKALQALRPLAARRAGRLWCVFGCGGDRDAGKRPLMGAAAEQQADRLLLTSDNPRSEDPDAILRQILQGISRRRAVRTEPDRAAAIAQAVAEAAPADVLLIAGKGHEDSQEVAGVRRPFSDWAHAQRAMQARQRAVPPRGAGA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Ideal protein should have a binding affinity for key enzymes in the Cell wall biogenesis pathway to enhance the overall efficiency of the pathway.2. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.3. The designed protein should contain one or more Mur ligase N-terminal catalytic, Mur ligase central domains that are essential for its function.4. A protein that can perform UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity in cytoplasm to support cell cycle, peptidoglycan biosynthetic process.5. The designed protein should have a high specificity for meso-2,6-diaminoheptanedioate binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MKLKDIISRIEYVKVVGDADRDIQTDSRKVKAGDAFVAWPGAATDGRAYVGKALEQGAAAVLVEAEGVPDVPDGVSVVVVKNTRESMAHISSNFFGYPAKKLKVIAVTGTNGKTTTVYLLKSMLEKAGKKVGLVGTIANYIGENKLKSERTTPESLELQKLFKDMVDANCEYCVMEVSSHSLQLDRVYGCEFEVGIFTNLTRDHLDFHKSFDNYYNAKFKLFERSKASVINVDDDYGYRVLKDVEKLENKEIKTYSINNNSDFKASDLMLKEGDIHFKINGEEFNSVLPGEYNVYNALGAVGISVAAGVPADVARAGIEHFPGVPGRLEPINSPEGYTAIVDYAHTPDALENVLIAIHDVLDGKGGKVITVCGAGGNRDKGKRPLMAVEAVKRSNKVILTSDNPRDEEPQAIIDDMIAGLDNTQKKKVLSIVDRREAIRTACMMAEKGDVILVAGKGHETYQITGDKVFDFDDREVAREALRGEM",
    "response#2": "MKLGEIFGLLGDNNLEVALEITDVTQDTRQLSDGCCFVAIKGLRFNGHEHVQEAVDAGARLIVVDEPVTVTGDVTVITVPDTHKALAQISARFYGFPSRRLRLVGVTGTNGKTTITHLISKILDDHDSKTGIIGTMYNKIGDEKIPTVNTTPDPHTVQKLLREMVDQDVMECAMEVSSIALVEGRAWGIDFDTAVFTNLTEDHLDFHKTVENYFDAKTLLFSQLGNSYHKTGKPKTAVINIDDPYGRRLIDKTAANVLTYGTHGQGLLQANNIRVTSHGTEFDLSIYGHSYPVKLQLMGDFNVYNALAAFGVGLELGFSLDQILECLPRLAGVPGRLEPINSPEGYTAIVDYAHTPDALENVLIAIHDVLDGKGGKVITVCGAGGNRDKGKRPLMAVEAVKRSNKVILTSDNPRDEEPQAIIDDMIAGLDNTQKKKVLSIVDRREAIRTACMMAEKGDVILVAGKGHEDYQILGTEKIHFDDREEARKALQDRLNS",
    "response#3": "MKLKDLLSCVEYTQISGVSDLDEIAGINIDSRKIKDGHLFVAIKGTQTDGHRFIPKAIELGATAILVEDMPEQLSEGVTYIQVKSTEDAVGKVATIFYNHPSQKLNLIGVTGTNGKTTTTHIIGHILRAQGYKVGLIGTIESIIGNKVIKTKNTTPESLDLQKLFSQMVDEGVEYCVMEVSSHALELDRVKGCEFDIGVFTNLTQDHLDYHKTMDNYYDAKKKLFVTGTNGKTTTAFLIDAILRASGRRTGLMGTIHYRVGDEVFEEPRTTSEGPDLQRYLRRMADAGASHAVMEVSSHGLALGRLSGCEFQAAIFTNLTQDHLDFHGDMESYFQAKSRLFKEFSPANAIVNADDAYGRRIAAEARGEVWTYALDAGADLGVENLSSSFAGMGFRMRTPAGSVDVETALIGRHNLSNILAASAAVTCLITDSRRVVPGALFFAIGGLRTDGNYYIEEAVDRGAVAIITEQDLGSHFPIDYIQVEDVRMALAVVAREFGLEKKDILNGLKSASAKFRIGRFQYFISDKEKVTAIVDYAHTPDALKNVLDTINTIRTKNETLITVVGCGGNRDATKRPKMAHIASANSDKSIFTSDNPRNEDPDAIIRDMEKGVESQHVSKVISIADRKQAIQTACVLANKNDIILIAGKGHEDYQILKDKTIHIDEREIVAEIIKERK"
  },
  {
    "reference": "MSKNEQTTSHTNTNATQNTTHFGYKQVPVDEKSNKVADVFHSVADKYDIMNDLMSGGVHRLWKKYTIDNSGAKKGDTILDLAGGTGDLAIKFSRIVGSEGQVTLSDINESMLENGRRRLTDMGIVGNIDYVLADAQELPFPDNHFDIVTMAFGLRNVTDKDKALRSIFRTIKPGGKLMVLEFSKPVLPGLSTFYDQYSFKLLPLMGKLIANDAESYQYLAESIRMHPDQETLKGMMEDAGFERTSYHNMTGGIVALHTGYKF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be designed to have high stability and activity towards a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine.2. The protein must exhibit the following characteristics: Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2)..3. We require a protein that can specifically bind to the metabolites produced in the Quinol/quinone metabolism pathway. The designed protein sequence is ",
    "response#1": "MNTEATHFGFQEVDEAEKTRRVGEVFHSVAARYDIMNDLMSFGMHRLWKRFAIELSGVRAGQRVLDIAGGTGDLAAKFSRLVGPTGQVVLADINESMLRVGRDRLIDRGVAGNVLFAQADAQYLPFASDSFDCITIAFGLRNVTDKQAALRSMLRVLKPGGSFVCLEFSKPVQPLLDKAYDAYSFRFLPTMGRIIAQDADSYRYLAESIRMHPDQETLKGMMEEAGFEQVDYFNLSGGIVALHRGFKF",
    "response#2": "MTIREQNTDQRTSHFGFQTVEESEKASRVRGVFDSVASKYDLMNDIMSFRLHRKWKRYAIDISSARPGNKVLDVAGGTGDLTAKFSRMVGKEGQVWLTDINSSMLHVGRDKLLDKGVVGNVSYVQADAQYLPFPDNTFDCITIAFGLRNVTDKDKALASMHRILKPGGKLLVLEFSKVAKPGLAKAYDAYSFKALPFMGKVVAGDADSYQYLAESIRMHPDQETLKQMMEDAGFVNCKYHNMTGGIVAVHTGVKP",
    "response#3": "MFRYKSITEALEDLKRGKMIIVVDDEDRENEGDLLMAAEKVTPEAINFMARYGRGLICMPMAEKLCDELGIELMVEKPTDPYGTAFTVSVDHVSTSTGISAFERAKTVEKVIDPNTKPEDFRRPGHMFPLVARDGGVLQRAGHTEAAVDLARLAGLYPAGVICEIIKEDGTMARVPELMEFAKKHNLKIITIADLIAYRRKYEKLIRCGEVGMTVPVYKENFLYLETKRDKMGHILGEVDSDSEAYLHIKREKMGHILDEVDSDSEAYLHIKREKMGHILDEVDSDSEAYLHIKREKMGHILDEVDSDSEAYLHIKREKMGHILDEVDSDSEAYLHIKREKMGHILDEVDSDSEAYLHIKREKMGHILDEVDSDSEAYLHIKREKMGHILDEVDSDSEAYLHIKRV"
  },
  {
    "reference": "MGRKWANIVAKKTAKDGATSKVYAKFGVEIYVAAKQGEPDPELNTTLKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSNVNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADSVFEQLLEADVDVDDVEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKLIDALESDDDVQKVYHNVADF",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein with DNA binding functions is required to enable regulation of DNA-templated transcription. The designed protein sequence is ",
    "response#1": "DLLETASKLLSELTHMACIISMPRRETLVFQHIEFLPLSDNRVLAILVTSDQEIHNKIIHTPHKFSAAELQTAANFFNAVCGGKDMVSAREHLRAELAKERDRLSEQLLQAGEIAEQALAEGGRSRKPFLVRGETNLMDFVELADVDRLRGLFEAFRQKESIVGLLDRCLESPGVKIIIGEESGFRPLEPCSLVTASYSVDNRVMGVLGVIGPTRMKYERVL",
    "response#2": "ETLLQQVARLLAVNTNYATMVTSPQYRSRKVKFLQVSEVDTTQILTIIVLEGNIVKNKIIAIAEPLDKETLLKLNIVLNTFLQGLDLSEINLALIRQMKEQAAEHSSVVSDILDAVAQAISEEDDIQIFTSGATNILKYPELNDMEKASELLYTLEEKKQLTNLMNNEVSSGENRGIQVYIGNETPVDSMKDCAVVTATYQIEEGVYGKIGIIGPKRMDYEKVL",
    "response#3": "DLLETASKLLSELTHMACIISMPRRETLVFQHIEFLPLSDNRVLAILVTSDQEIHNKIIHTPHKFSAAELQTAANFFNAVCGGKDMVSAREHLRAELAKERDRLSEQLLQAGEIAEQALAEGGRSRKPFLVRGETNLMDFVELADVDRLRGLFEAFRQKESIVGLLDRCLESPGVKIIIGEESGFRPLEPCSLVTASYSVDNRVMGVLGVIGPTRMKYERVL"
  },
  {
    "reference": "MSSTKSDFEIIREYIIGDGKDISVGKAPEGNVRLEITHSVLTGESLGTNNFKNFSLDLKIKDFKEKLYRFVGTEPKYMELILRDENKVNDICKIDDDDDKTLGSYEPKDGMNVHIIDKDPNNFVSELQDISKAPKPVISEEDYNKREGTYKKWKEENQLKKENDTTATTVTATTTTTNNATDTEIEIKVGDRCKVISDDPTNYDERLGKVQYVGTVEFSSGVWIGVELDLPLGKNDGSVKGKQYFQCSPKYGCFAKPKNVLVGDYPEEEI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein have CAP-Gly domains with specific sequence motifs that are crucial for its activity.2. The designed protein should have a specific function within the microtubule, cytoplasm.3. For general function, the protein need meet that Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. The designed protein sequence is ",
    "response#1": "PRLHFFVPGFAPLASRTSQSYQSCTILELTRQMFDAKNMMAACDPSHGRYLTVAAMYRGRVSMKEVEDRILETQTRNSTYFVEWIPNNVKTAVCDIPPIDFKVAGTFIGNTTAIQELFTRVR",
    "response#2": "LVHMQAGQCGNQIGSKFWETISQEHGIDEMGSYHGDSDLQLERINVYYNEGQGGKYVPRALLIDLEPGTMDSVRSGPLGKLFRPDNFIFGQSGAGNNWAKGHYTEGAELIEEVLDVVRKECEACDCLQGFQLCHSLGGGTGSGMGTLLIAKIREEYPDRIMTSFSVVPSPKVSDTVVEPYNATLSVHQLVESTDETFCIDNEALYDIG",
    "response#3": "PRLHFFVPGFAPLASRTSQSYQSCTILELTRQMFDAKNMMAACDPSHGRYLTVAAMYRGRVSMKEVEDRILETQTRNSTYFVEWIPNNVKTAVCDIPPIDFKVAGTFIGNTTAIQELFTRVR"
  },
  {
    "reference": "MSFDLLKDINKKVHFIGIGGVSMSGLAAVLLNAGYKVSGSDSKESEITNRLKEEGAKIYIGHSKDNLQDVDVVVYTAAIPSDNPEIIKAKEDNLILMDRAEFLGQIMKGHKFNVAVAGTHGKTTTTSMISHVALSADLDPTILVGGDLDIIHGNFRVGNSEYFITEACEYKQSFLRFFPYVGIILNIDADHLDFYKDINHIKDTFKQFVRLIPNDGYIIGNADDEKVMEVLEVAKCNVLTYGINNGDIQARNIEFNEKGCATFDVFRNEEKLLSLSLNVPGMHNVSNSLSAVCLAEIFNINADAIVSGLSSFGGAHKRFEYKGTKNDITVIDDYAHHPVEIKATLSTAKKMNHNKIVCVFQPHTYTRTKTLFNDFVKCFDDCDELVLMDIYAAREKDLGEINSDQLGDAIRSHGVKCTNVHSHEEALEYVNANLSKGDLLLTVGAGDVVKVGELFLN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in peptidoglycan biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.2. For general function, the protein need meet that Cell wall formation. The designed protein sequence is ",
    "response#1": "ITGTNGKTTVSTLLYQIFEAVGRDTGLIGTVETRIGAEGLKSSRTTPEASDLQALAAVMRERHMRHLVMEVSSHALTLKRMKGSHFAIAAFTNLTQDHLDFHKDMDAYFSAKAELFTSEYAEKAFINIDSPYGQRLAEQVKIPVTRISRTNKAAEWHFTQTHNVARGIEFSARGTGGILIDSHTQLSGGFNLDNLLLALAIAGE",
    "response#2": "VTGTNGKTTTATLIKEMLRRAGHKVGLIGTVQYEIGDEILPSPNTTPLTKSYFELLAKMADAGVDTVVMEVSSFGLEQHRIGPTHFDIAVFTNLTQDHLDIHGTMENYYQAKRMLFDVCDYAIVCGNDTEYGTRLFREITCEKCTYGVFRPETGRLDVMADAVELSTEGTKFTLHLHGETMPMSMRMTGLFNVSNAVAAIAGV",
    "response#3": "ITGTNGKTTVSTLLYQIFEAVGRDTGLIGTVETRIGAEGLKSSRTTPEASDLQALAAVMRERHMRHLVMEVSSHALTLKRMKGSHFAIAAFTNLTQDHLDFHKDMDAYFSAKAELFTSEYAEKAFINIDSPYGQRLAEQVKIPVTRISRTNKAAEWHFTQTHNVARGIEFSARGTGGILIDSHTQLSGGFNLDNLLLALAICV"
  },
  {
    "reference": "MQDLHKDLKSLTLDQLENIVEGFGSKSFHAAYLYEYIHSRDGVYVNDVTTLSKGLRAQLIENGWYISQLKTVEKFTDPDGTLKYLFENEDGGRFESVLLNDDNRLTLCISSQCGCRMGCKFCATARLEFRQDLSVGQIVDQVYRVNADAGRIDNVVFMGMGEPFDNTENVFAAADILNSAKGLNIGARHITISTCGIPGPIEALACHDKQYRLAVSLHAADDQTRRKLMPVNSKYPLKDLLSAVKNYCRQTNRRVTFEYCLIDGVNDSADNAKQLVKLIEGIKCNVNLIEFNPFEGCSFKSSPRRKIQAFRDVLDMAGIEAHTRYKRGRSIKAACGQLGADWLEKQLCS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that can perform rRNA binding, tRNA binding in cytoplasm to support rRNA base methylation.2. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.3. The presence of Radical SAM core domains is critical for the interaction of the designed protein with its intended targets.4. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs..5. The protein must be engineered to have a cooperative binding mechanism with [4Fe-4S] cluster to ensure proper regulation. The designed protein sequence is ",
    "response#1": "VTMCVSSQAGCGMNCPFCATGQAGLDRNLSTAEIVHQIVDGARALRDGEVPGGEARLSNIVFMGMGEPLANYRRVVGAIRRLTDEAPDGVGLSQRGITVSTVGLVPAMLRFADEGFSCRLAVSLHAPDDELRDTLVPVNTRWKVREVLDAASTYAERSGRRISIEYALIRDINDQEWRADLLGRLLKGRGAHVNLIPLNPTPGSKWTASRPEDERAFVAAG",
    "response#2": "EKTRRTLCISSQVGCALGCVFCATGRMGFRRNLSAGEIAGQVREAMRRDPAAQPTNVVFMGMGEPLLNWDAVDTALTILNHPDGLGIGARHITVSTVGILPHLARLAQRPEQFRLAVSLHAPTAELRRELMPIEKKYSLPEVLQALTQFRRRVTLEYVLIGGKNDALAQADELAKLARPLGALVNLLPLHPGGAPDLTPSDRPRMLAFERRLKSHGVEAVLRRSRGLDIE",
    "response#3": "VTMCVSSQAGCGMNCPFCATGQAGLDRNLSTAEIVHQIVDGARALRDGEVPGGEARLSNIVFMGMGEPLANYRRVVGAIRRLTDEAPDGVGLSQRGITVSTVGLVPAMLRFADEGFSCRLAVSLHAPDDELRDTLVPVNTRWKVREVLDAASTYAERSGRRISIEYALIRDINDQEWRADLLGRLLKGRGAHVNLIPLNPTPGSKWTASRPEDERAFVAAATP"
  },
  {
    "reference": "MSLVANEEFQHILRVLNTNVDGKQKIMFALTSIKGIGRRLANIVCKKADVDMNKRAGELSAAEIDNLMTIVANPRQFKIPDWFLNRQKDYKDGKYSQVVSNALDMKLRDDLERLKKIRNHRGLRHYWGLRVRGQHTKTTGRRGKTVGVSKKR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The ideal protein would be one that plays a critical role in translational initiation through rRNA binding within cytosol, plasma membrane, cytosolic ribosome, nucleolus, cytosolic small ribosomal subunit.2. The protein must exhibit the following characteristics: Located at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA.. The designed protein sequence is ",
    "response#1": "LESRLDNLVYRLGLARTRRQARQLG",
    "response#2": "GRRLQTIVYKKGLARTIYQARQLITHGHIAISGRKVTSPGYVVLRGEEDLIDYYPTSPFKQNPI",
    "response#3": "MRLDNIVFRLHMAPTIKAARQLISHGHILINKKKVTIPSYQCEPKDIITVAPKIISMELVSRFLV"
  },
  {
    "reference": "MSTKYVFVTGGVVSGLGKGITAASLGRLLKNRGYKVANQKFDPYINVDPGTMSPYEHGEVFVTDDGAETDLDLGHYERFTDENLTKNSSITTGRIYQRVIEKERRGDYLGKTVQVIPHITNEIKEKVYSLGKDGADVVITEIGGTVGDIESLPFLEAIRQVGIENNPEDVVYIHVTLLPYITGSNELKSKPTQHSVKELQSLGIKPDVLVCRTELPITENIRNKIALFCNVRPENVIANMTASNLYEVPLMLEKEGLATSICKHLKLEKIEPKNEEWEKMIEHFKNVDKKYKDEKVKKVKIAIVGKYIKLEDSYLSVVESIKHAGYANEVDVEIGFVDSETINAENAKEKLEKFDGIIVPGGFGNRGIEGKIQTVKYARENNVPFLGICLGMQMAVIEFARDVLDLKDADSVEFNPDTKNPVIHIMEDQKNITKKGGTMRLGAYPCVLEKGTLASNLYGSEEISERHRHRYEFNNDYKKKMEEKKMVFSGTSPDGNLVEIVEIKNHPYFIAVQFHPEFKSRPDRPQPLFVGLVKATKNRI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should contain one or more CTP synthase N-terminal, Glutamine amidotransferase domains that are essential for its function.2. The protein design must be able to perform ATP binding, metal ion binding in order to contribute to glutamine metabolic process, 'de novo' CTP biosynthetic process.3. The protein should be optimized for activity in Pyrimidine metabolism, CTP biosynthesis via de novo pathway, CTP from UDP: step 2/2 pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The active site of the protein must be engineered to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).5. The protein should be able to bind ATP, Mg(2+), CTP, L-glutamine ligand in a variety of conditions6. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen The designed protein sequence is ",
    "response#1": "CVTGGVVSSLGKGIAAASLGAILEARGLKVSMVKLDPYINVDPGTMSPFQHGEVFVTTDGTETDLDLGHYERFVRTTTSRNSNFTTGRIYERVIAKERRGDYLGATVQVIPHITDEIKHCIRLGAGDADVCMVEIGGTVGDIESLPFLEAIRQLGVELGRNNVIYMHLTLIPYIPSSGEIKTKPTQHSVKELRSIGIQPDILLCRAHHPLPEEQRRKIALFTNVEERAVISAVDADDLYKIPLLLHEEGLDEIVVDKLRLHDSYKSINESFVHAGAMNRCKVDLQFIHSEDIERGDINELLGSLSGILVAPGFGDRGIEGKILAAQYARENRVPYFGVCLGMQCAVIEFARNVLGLKEAHSTEIARNTPYPVIDLMEDQKGITNKGGTMRLGAYPCNIKKGSLAWEAYGKLKVSERHRHRFEFNNQYLKAFEKAGMVATGINPDTNLVEIMEIPSHPWFVGVQFHPEYRSTVKEPHPLFVAFVLNQLNS",
    "response#2": "KFIFVTGGVVSSLGKGIASASLGMLLQSRGLKVTILKMDPYLNIDPGTLSPYQHGEVYVTDDGAEADLDLGHYERFLNIDMSKHNNATTGQIYYAVIKRERNGDYLGKTVQVIPHITDEIKRRILDLSKGKEKVDVVITEVGGTVGDIESLPFLETIRQFRLDVGSENLLNVHLTLVPFISAAGEYKTKPTQHSVMRLREIGIQPDILLCRAQAPLDDELKRKIALFCSVATNSVISAPDVATIYEVPLMFHRGGLDEIIVNRLALHDAYKSIAESFLHAGSVNECKVNIQWIHSEKLTDKNYQEELKDLKGILVAPGFGHRGLEGKILATKYARENNVPFLGICLGMQVSVIEFARNVLKLEDADSLEMNPKSPYPVIDLMEEQKNVTEKGGTMRLGAYDCVLKDGSKALKAYGQKEISERHRHRYEFNNEFLEQFEAAGMKATGMNPDTGLVEVVELEGHKWFVGVQFHPEYKSTVIKPHPLFVEFVKSAVAKQDSYKSLNEALYHGGFAHRLKVNIKWIEAEALEEPGGVALLEGADAILVPGGFGDRGTRGMMKAAQVGREREIPYFGICYGFQWATVEYARNVAGLTDADSTECTPDTPTKVIYKLRDLLGVDELGGTMRLGSYACELAPGSLAQKVYGTHLIHERHRHRYEFNCLYERPLAQHGLKITGRSPDGKFAEIAELPGHPWYLAVQFHPEFRSKPTRPHPLFASFVKDAYKSLIEALSHGGIANKVKVNLDWIESEVFEREDPAPFLEHVNGILVPGGFGQRGAEGKIKAVRFARERRVPYFGICFGMQMAVIEAARNLVGIEGANSTEFGETKEPLVGLMTEWLRGNELEKRSKAGDLGGTMRLGAFPAALRPGSRVSKIYGNALEISERHRHRYEVNTAYKDRLEQHGLRFSGMSPDGVLPEIVEYEDHPWFVGVQYHPELKSRPLDPHPLFASFI",
    "response#3": "VTGGVVSSLGKGIAAASLGAILEARGLKVSMVKLDPYINVDPGTMSPFQHGEVFVTTDGTETDLDLGHYERFVRTTTSRNSNFTTGRIYERVIAKERRGDYLGATVQVIPHITDEIKHCIRLGAGDADVCMVEIGGTVGDIESLPFLEAIRQLGVELGRNNVIYMHLTLIPYIPSSGEIKTKPTQHSVKELRSIGIQPDILLCRAHHPLPEEQRRKIALFTNVEERAVISAVDADDLYKIPLLLHEEGLDEIVVERLGLEAPKPDLTQWEAMVERLKNPTHTDSYKSLNEALYHGGFAHRLKVNIKWIEAEALEEPGGVALLEGADAILVPGGFGDRGTRGMMKAAQVGREREIPYFGICYGFQWATVEYARNVAGLTDADSTECTPDTPTKVIYKLRDLLGVDELGGTMRLGSYACELAPGSLAQKVYGTHLIHERHRHRYEFNCLYERPLAQHGLKITGRSPDGKFAEIAELPGHPWYLAVQFHPEFRSKPTRPHPLFASFVDFIGAAIKIAVCGKYVELHDSYKSIAESFVHAGVENNVRVKLEWIEAEKIEQQNPAELLRGTSGLLVPGGFGSRGSEGKVVAIQYAREQQIPFFGICLGLQCAVIEFARNVCGLANANSTEFNEATPNPVIDLMEEQKRIKSKGGTMRLGAQPCEIKKATKAHRIYGKELVYERHRHRYEVNNEFKAKLEQHGMALSGINPELDLVEMIELPNHPWFVACQFHPELKSRVLNAHPLFREFLSAASRYNPETT"
  },
  {
    "reference": "MSVLKLLCCLLITYHLSEGKKIKGNRAVVDTQQRKVPKAGELYVSSSVFRLFVEGEDTCTLDPLQLHTLTSCGFNSSNPLIIITHGWSVDGMMDSWVLRLAATLKKNLIDVNVVITDWLSLAHQHYPIAVQGTRTVGKDIAHLLQSLQKQLQYPVSKAHLIGYSLGAHISGFAGSYLEGSEKIGRITGLDPAGPLFEGMSPTDRLSPDDAEFVDAIHTFTHERMGLSVGIKQAVGHYDFYPNGGDFQPGCDLQNIYEHIAQYGLLGFEQTVKCAHERSVHLFIDSLLNKDKQSMAYRCSDNSAFDKGTCLDCRRNRCNILGYNINKVRTSNSKRLYLKTRSRMPYKLYHYQFRIQFVNQTERVEPSLTIALTGTKEESGDFHITFPAEIIVNKIYTFLITLDKDLGELMLLKFHWEGSALWKNVWNRVQTIIPWGGHKTKPQLTVGKIAVKAGETQERTTFCAMENDVQDVKISEDKVFVRCEDATPKQRRRKLNRLVLVT",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The design of the protein must focus on the precise arrangement of amino acids in the Charge relay system, Nucleophile to enable effective catalysis of a triacylglycerol + H2O = a diacylglycerol + a fatty acid +   H(+).2. Design the protein with a signal peptide incorporated.3. The designed protein should have phosphatidyl phospholipase B activity, phosphatidylserine 1-acylhydrolase activity, 1-acyl-2-lysophosphatidylserine acylhydrolase activity, triglyceride lipase activity, lysophospholipase activity within high-density lipoprotein particle to regulate lipid metabolic process.4. The protein should have a high affinity for Ca(2+).5. The designed protein should contain one or more PLAT domains that are essential for its function. The designed protein sequence is ",
    "response#1": "RMFLEGEDNCTLDPLQLHTLTSCGFNSSNPLIIITHGWSVDGMMESWVHRLATAMKSNLMNINVVITDWLSLAHQHYPKAAQSTRTVGKDIAHLLHSLQVHYQFPFKKVHLIGYSLGAHISGFAGSNLESSEKIGRITGLDPAGPLFEGMSPTDRLSPDDAEFVDAIHTFTQEHLGLSVGIKQAVAHYDFYPNGGDFQPGCDLHNIYEHISQYGLLGFEQTVKCAHERSVHLFIDSLMNKDKQSMAYRCSGKSAFEKGVCLDCRKNRCNTLGYNIRKVRSSTSKRLYLNTRSRMPQYHEVNHHYQFRIQFINQMEQIEPFLTISLNGTKEESGNHGISFTENISGNKTYTFLITQDRDLGDLMMLKLRWEDPALWKNVWNKVQTIIPWGSRRRKPRLTVGKISVKTGETQARTYFCAMMNDGQEVEVSQDKVFVC",
    "response#2": "RMFLEGEDNCTLDPLQLHTLTSCGFNSSNPLIIITHGWSVDGMMESWVHRLATAMKSNLMNINVVITDWLSLAHQHYPKAAQSTRTVGKDIAHLLHSLQVHYQFPFKKVHLIGYSLGAHISGFAGSNLESSEKIGRITGLDPAGPLFEGMSPTDRLSPDDAEFVDAIHTFTQEHLGLSVGIKQAVAHYDFYPNGGDFQPGCDLHNIYEHISQYGLLGFEQTVKCAHERSVHLFIDSLMNKDKQSMAYRCSGKSAFEKGVCLDCRKNRCNTLGYNIRKVRSSTSKRLYLNTRSRMPVRASLSMSVTGTKGETTPQSVTLSQEYAENTTYSSLFAVDSELGELQALRLHWKEERSWWTHVTDFMSGSGSRQDTELSVARIRLKSGESQEKTWFS",
    "response#3": "PYVSSSVFSLFLQGEDTCALDPLQLQTLTSCGFNSSNPLIIITHGWSVDGMMEGWVLRLASTLKTSVININVVITDWLTLAHQHYPIAAQSTRTVGKDIAHLLQLLKEYYQFPPKKVHLIGYSLGAHISGFAGSYMESSEKIGRITGLDPAGPLFEGMSPTDRLSPDDAEFVDAIHTFTHERMGLSVGIKQAVAHFDFYPNGGDFQPGCDLKNIYEHITQYGLLGFEQTVKCAHERSVHLFIDSILNKDKQSMAYRCSDNSVFDKGVCLDCRKNRCNTLGYNIKKVRTVTSKRFYLKTRSRMPYHYQVKVMLVNLIEPVRASLSMSVTGTKGETTPQSVTLSQEYAENTTYSSLFAVDSELGELQALRLHWKEERSWWTHVTDFMSGSGSRQDTELSVARIRLKSGESQEKTWFCGQAGNITHLKPLEEQEFVRCK"
  },
  {
    "reference": "MNFSESFKLSGLLCRFSPDGKYLASCVQYRLVIRDVTTLQILQLYTCLDQIQHIEWSADSLFILCAMYRRGLVQVWSLEQPEWHCKIDEGSAGLVASCWSPDGRHILNTTEFHLRITVWSLCTKSVSYIKYPKACQQGLTFTRDGRYLALAERRDCRDYVSIFVCSDWQLLRHFDTDTQDLTGIEWAPNGCVLAAWDTCLEYKVLLYSLDGRLLSAYCAYEWSLGIKSVAWSPSSQFLAIGSYDGKVRLLNHVTWKMITEFGHPATINNPKTVVYKEAEKSPLLGLGHLSFPPPRAMAGALSTSESKYEIASGPVSLQTLKPVADRANPRMGVGMLAFSSDSYFLASRNDNVPNAVWIWDIQKLKLFVVLEHMSPVRSFQWDPQQPRLAICTGGSKVYLWSPAGCVSVQVPGEGDFPVLGLCWHLSGDSLALLSKDHFCLCFLETKERVGTAYEQRDGMPRT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Required for ciliogenesis and involved in the removal of the CEP97:CCP110 complex from the mother centriole; Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP, CEP290 and PIBF1/CEP90.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm that is critical for protein localization to mitotic spindle pole body. The designed protein sequence is ",
    "response#1": "KVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPSILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRVKPFSENEARHFMHQIITGMLYLHSHGILHRDLTLSNLLLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDVWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPSFLSIEAKDLIHQLLRRNPADRLSLSSVLDHPFI",
    "response#2": "FRVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPSILELYNYFEDNNYVYLVLEMCHNGEMNRYLKNRRKPFSENEARHFMHQIITGMLYLHSHGILHRDLTLSNLLLTRNMNIKIADFGLAAQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDIWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPTFLSREAKDLIHQLLRRNPADRLSLSSVLDHPFL",
    "response#3": "RVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPSILELYNYFEDNNYVYLVLEMCHNGEMNRYLKNRRKPFSENEARHFMHQIITGMLYLHSHGILHRDLTLSNLLLTRNMNIKIADFGLAAQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDIWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPTFLSREAKDLIHQLLRRNPADRLSLSSVLDHPFL"
  },
  {
    "reference": "MTALITQEIGSFRKPDYLAKEFHKIERTPKFTELAERATRETLEVFERSGLDNIGIGGEMFRWEMYEHPAERIKGIIFYGMVRSFDNRYYRKGSAIDRLERREPFHVDEVKFVAGTTKKPLKVPITGPYTMMEWSFNDYYDSREDLAMEFARIINEELKDIASVWKQVSGGRRLEIQIDEPATTTHPDEMDIVVDSINRSVQGVDGEISMHVCYSSDYRLLYDRIPDLKIDGYNLEYSNRDTLERGLTDDKRVGFQDLKYFAQINESLQRKKFIGIGVTDVHIDYVEPVELIEDRINYALKIIGDPDLVRINPDCGLRTRSREIGEQKLRNMVMARNNILKQL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Catalyzes the transfer of a methyl group to L-homocysteine resulting in methionine formation.2. The protein must have high specificity towards 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity, zinc ion binding.3. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "MITTVVGSYPATPREPETLRERISSFIGSYDACRPAIETAVRDQVRAGVDIISDGQVRGDMVGHFAEAMGGMMVKDGVSIIFSRITPPAGSIGSDDLIYAIKILRKLTDDESKGVKGIITGPSTMAHASKIEGFYSPQKRERAIMDMADALRVEAEHLQDAGALMIQIDEPFLSTGMVDMGTARRAVKRISGALDVDVSLHVCGDISGVIGELLTFPVDMLDLEFAGRPSNLEVLAEKWRGDKKLGFGCVDTSTEVVESTDTIKNLIERGADIAGEDNLYIDPDCGMRKLPREAAFSKLRNMVKAAA",
    "response#2": "MITTVVGSYPATPREPETLRERISSFIGSYDACRPAIETAVRDQVRAGVDIISDGQVRGDMVGHFAEAMGGMMVKDGVSIIFSRITPPAGSIGSDDLIYAIKILRKLTDDESKGVKGIITGPSTMAHASKIEGFYSPQKRERAIMDMADALRVEAEHLQDAGALMIQIDEPFLSTGMVDMGTARRAVKRISGALDVDVSLHVCGDISGVIGELLTFPVDMLDLEFAGRPSNLEVLAEKWRGDKKLGFGCVDTSTEVVESTDTIKNLIERGADIAGEDNLYIDPDCGMRKLPREAAFSKLRNMVKAAQ",
    "response#3": "LPTTTIGSFPQTTEVRQARAAFAKGQISDATYEAFLRQETARAVAWQETVGLDVLVHGEFERNDMVQYFGEQLAGFAFTQSGWVQSYGSRCVRPPILFGDVSRPAPMTVDWWRYAQGLTRRPMKGMLTGPVTILNWSFVRDDLPRETACRQIALAIRDEVVDLEATGAAIIQIDEAALREGLPLRRGDWDAYLDWAVESFRLAASGVADATQIHTHMCYSEFNDIIAAIGAMDADVISIETARSKMELLDAFVGYAYPAQIGPGVYDIHSPRVPAVEEMTTLLAAARQRLAGEQLWVNPDCGLKTRKWPETQAAIVNMTQ"
  },
  {
    "reference": "MRNGPRVQPNGERAIVQIVVRDNNVEQALRALKKKMQREGTFREMKRRNHYEKPSEKKARQKAEAIRRARKLARKRAQREGLIAKRGGTTRR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be able to modulate translation in a way that leads to a desirable outcome.2. The protein should exhibit Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "STKRRR",
    "response#2": "SWIIP",
    "response#3": "MPNSKTNGKFRYNFHRRDWRG"
  },
  {
    "reference": "MTKFIFVTGGVVSSLGKGLTSASVGMLLEQRGLRVKMQKLDPYINVDPGTMSPYQHGEVYVLDDGSETDLDLGHYERFTNSPLTRDSNYTTGQIYLSVIEKERKGEFLGKTVQVIPHITNEIKRVIQKLASDDTDVVITEIGGTVGDIESQPFLEAIRQFSLDVGKENCLYMHLTLVPYLKAAGELKTKPTQHSVGQLREIGIQPDILVCRTEKTISREDREKIGLFCNVPIDAVIEEKDKDFSIYEVPLSLVHNQLDELIIRKLRLEAESLDLDRWHDLMHRLRNPEHEIAIAVVGKYAEHRDAYKSIYEAIDHAGIHHRAQIRIASIQSEQVESEGPERTLAGYDGILVPGGFGERGIEGKVEAIRFARERNIPYFGICLGMQCAAIEYARNVVGLTSAHSSEFDRNSEHHVICLLDEQQNVTDMGGTMRLGAQPAVLDKESNAFRCYGDTEIQERHRHRYEFNNQYRQQFAAHGMMFGGLSPDGGLVEIIEIPEHAWFVAVQFHPEFKSKPTCAHPLFAGFVGAAIQRRQLKSERVQEELDHAKQIDS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should interact with enzymes and substrates within the CTP from UDP: step 2/2 pathway to enhance or regulate its activity.2. The designed protein must be optimized for CTP synthase activity, metal ion binding.3. The designed protein have CTP synthase N-terminal, Glutamine amidotransferase domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "VPIAMVGKYTKLKDSYISITEALKSAGVFTSVMPKLIWVEATDIENGQIKVEDVLQDVKGAVVLPGFGARGTEGKIKAIKYLRENNIPMLGVCFGMQLSVVEFSRNVLGLKDANSTEINPNTPYPVIDLLPNQKNIDYMGGTMRLGASKINVVKNTTVYDLYRKEIVYERHRHRYEVNLKFLDKLIDAGIKVSGWSDQGLVEFIELSKKDHKFFVGTQPHPEFTSRPLNPNPLYVGLY",
    "response#2": "QDAYKSVAEALKHGGLRQRTKVRLKWLESSDVEKRGPSALEGVDGVLVPGGFGDRGFEGKIAGARYARENGVPYFGICYGMQAAVIEFARNVAGLENANSTENDKATPHPVVGLITEWRTASGELERRSEDSDLGGTMRLGLQEQRIKPGTLAHELYGKDVVGERHRHRYEFNNRYRTQLEDAGLVISAKSIDDLLVEMIELPRDVHPWFLACQAHPEFLSTPRDGHPLFVGFITK",
    "response#3": "VTIAIIGKYVDHQDAYKSVAEALKHGGLRQRTKVRLKWLESSDVEKRGPSALEGVDGVLVPGGFGDRGFEGKIAGARYARENGVPYFGICYGMQAAVIEFARNVAGLENANSTENDKATPHPVVGLITEWRTASGELERRSEDSDLGGTMRLGLQEQRIKPGTLAHELYGKDVVGERHRHRYEFNNRYRTQLEDAGLVISAKSIDDLLVEMIELPRDVHPWFLACQAHPEFLSTPRDGHPLFVGFMR"
  },
  {
    "reference": "MNKEISTADILSGGESYSGISAVEKNAKAMMKVYNAIKTSFGPLGLDKMCVDSAGEVSITNDGATILQNMLIDDPAAKILVDLATQQDHEVGDGTTSVVLIAVSLIEKGAKLIASGVHPSVVVSGYKMAFNECVQFIKKSMSKSTLNLGSKALRNVVETSISSKVISSESEVFCGIVIDALKCIESVDENRKNMYPIEDINILKHPGGSMKESFLHQGYALNCSLASNFMKRQVKKPKILCIDFGLQKYKNPLTVSIVVDDPNKLEDIRKKELEITRRQIKTIIDSGANVVLTTRGIDDMCTKLLVEADVVGIRRCKKEDLLVIAKATGTSLVSSIADISGADSISSLGFADKFEVVQIGEEECVLINGLKKKMASIILRGANCQLLDEMQRSVHDAVCVLKRTLESNSVVPGGGAVECALSLMLEKFAFTVNSKEHVAIHRYAESLLSIPKILSTNAGLDSNELVANLLSSQSREMANSSGSKFLGIDVTSGKIQDNFEFGIIEPSVNKMKSLKAATEAAISILRINEVIILPPDQSKN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be optimized for ATP binding. The designed protein sequence is ",
    "response#1": "VAIIGGGAYTGAPYLAGMAALRAGCDLVYVCCPSSVWSVVASFSPDLIVRKISGEFLTVEGLKEVEDIIEKADAIVVGPGLGKRKEVLEACKFIFENYDKKFVYDADAIEVLKGKKCNQKVIITPHSGEFKRLTGIDLPDDIEERSRIVKKEAKKLEATILAKGAIDIISDGKRLKMNKIHNEGMTVGGTGDTLAGICAAMLAKKIAPFYSACISAFINGMAGNMAYEEKGNIVATDMIEKIK",
    "response#2": "VGPGELILYPRRRKESHKGENGIVAIIGGGAYTGAPYLAGMAALRAGCDLVYVCCPSSVWSVVASFSPDLIVRKISGEFLTVEGLKEVEDIIEKADAIVVGPGLGKRKEVLEACKFIFENYDKKFVYDADAIEVLKGKKCNQKVIITPHSGEFKRLTGIDLPDDIEERSRIVKKEAKKLEATILAKGAIDIISDGKRLKMNKIHNEGMTVGGTGDTLAGICAAMLAKKIAPFYSACISAFINGMAGNMAYEEKGNIVATDMIEKIPLVIKKK",
    "response#3": "VGPGELILYPRRRKESHKGENGIVAIIGGGAYTGAPYLAGMAALRAGCDLVYVCCPSSVWSVVASFSPDLIVRKISGEFLTVEGLKEVEDIIEKADAIVVGPGLGKRKEVLEACKFIFENYDKKFVYDADAIEVLKGKKCNQKVIITPHSGEFKRLTGIDLPDDIEERSRIVKKEAKKLEATILAKGAIDIISDGKRLKMNKIHNEGMTVGGTGDTLAGICAAMLAKKIAPFYSACISAFINGMAGNMAYEEKGNIVATDMIEKIPLVIKKFE"
  },
  {
    "reference": "MGRLKDKFNESIKPALVKEFDIKNPMLVPALEKIVISVGAGDFAKDQKVLQNMADTISLIAGQKAVITNAKKSVAGFKVREGFPVGIKVTLRKEQMYAFLDKLISIALPRVKDFRGLPKNGFDGRGNYNFGLSEQLMFPEVEYDKILRTHGMNITIATTAKNDKEAFKLLELFGLPFAKGK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein can be targeted to ribosome and perform rRNA binding. The designed protein sequence is ",
    "response#1": "GRCAETGRPRGYYRKFKLCRNIIG",
    "response#2": "LKSTPAAVLLKKAAGLKSGSPRPNTQKVGTVTRAQLEEIVVAKQADLTASNMDAAVRTIAGSARAMGLEV",
    "response#3": "MSKSTPAAVLLKKAAGLKSGSPRPNTQKVGTVTRAQLEEIVVAKQADLTASNMDAAVRTIAGSARAMGLEI"
  },
  {
    "reference": "MNFWTLLLPPIAGTVIGYFTNDIAINMLFRPYKAIYIGDRRLPFTPGLIPANQDRLARNISRIIMGSLLTPEEIQKLAQKLLQTERIEAAIRWLLQLAFAQIQGEQEQKTAGILAAILRDLASESLPRLIKVWSREDTFLEAQVYQIFDQLLLDFKLTEAQARQLTDWLLRTVLTPDILRQATIDFLTDKNIEIIDTSLREKTSGTYWVVANLFGVKNSLTRLRAFCLEEREIANARLQELILTLEIRLKLRLWLQGLSLQNLPVSTVRQLRKSFHQTIRQYFQNKGAGLMEYIGESVDWDNLSVVILRRLQASQVLDSSLGVVSQELSLLLDRYLEKDLEKIISQVIPILAIDQVIIERVNNTSPRELERAIQGIVKNELQAIVNLGGVLGFLVGVAQSVILLLN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The designed protein localizes to the plasma membrane. The designed protein sequence is ",
    "response#1": "KGALLVCLAATMWGFDGIALTPRLFSLHVPFVVFILHLLPLILMSILFGKEEVKNIKKLQKNDLFFFFCVALFGGCLGTLCIVKALFLVNFKHLTVVTLLQKLQPIFAIILARLLLKEKLKRAYLFWGFLALLGGYLLF",
    "response#2": "KGALLVCLAATMWGFDGIALTPRLFSLHVPFVVFILHLLPLILMSILFGKEEVKNIKKLQKNDLFFFFCVALFGGCLGTLCIVKALFLVNFKHLTVVTLLQKLQPIFAIILARLLLKEKLKRAYLFWGFLALLGGYLLF",
    "response#3": "KGALLVCLAATMWGFDGIALTPRLFSLHVPFVVFILHLLPLILMSILFGKEEVKNIKKLQKNDLFFFFCVALFGGCLGTLCIVKALFLVNFKHLTVVTLLQKLQPIFAIILARLLLKEKLKRAYLFWGFLALLGGYLLI"
  },
  {
    "reference": "AGLLAGSWIPDWTFVSVPPLVTLWYTLTKEPIPGEDVYYVDGACNRNSREGKAGYITQQGKQRVEKLENTTNQQAELTAIKMALEDSGPRVNIVTDSQYA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that RNase H activity is associated with the reverse transcriptase.2. A protein with RNA-DNA hybrid ribonuclease activity, nucleic acid binding, aspartic-type endopeptidase activity, RNA-directed DNA polymerase activity is needed to enhance suppression by virus of host gene expression, proteolysis in a specific context. The designed protein sequence is ",
    "response#1": "MGKTYSSPVNPIPKAPKGLAIHHWLNFLQAAYRLQPGPSEFDFHQLRKFLKLAIKTPVWLNPINYSVLARLIPKNYPGRVHEIVAILIQETR",
    "response#2": "MGKTYSSPVNPIPKAPKGLAIHHWLNFLQAAYRLQPGPSEFDFHQLRKFLKLAIKTPVWLNPINYSVLARLIPKNYPGRVHEIVAILIQETG",
    "response#3": "MGKTYSSPVNPIPKAPKGLAIHHWLNFLQAAYRLQPGPSEFDFHQLRKFLKLAIKTPVWLNPINYSVLARLIPKNYPGRVHEIVAILIQETR"
  },
  {
    "reference": "MTKRYAVVLAAGQGTRMKSKLYKVLHPVMGRPMVQHVVDQLNKLELEKLITVVGFGAEKVQEQLGEAVEFVIQEEQLGTGHAVLQAEDLLQDKEGTTIVVSGDTPLLTNHTLEALMNHHDSEGAKATILTAEAPDPTGYGRVIRNENNTVEKVVEHKDASAQELAISEINTGTYCFDNQALFTALKQVSNENAQGEYYLPDVLEILKKSGEKVGAFQTGTFDETLGVNDRVALSQAEKLMKKRINEQHMRNGVTITDPENTYIAPEVVIEQDVVIYPGTILNGKTVIKSDAVIGPNSEIENCTVGVGTVIRQSVAKDSEIGERVQVGPFAHIRPQSSLGDQVKIGNFVEVKKAAVEENSKVSHLSYIGDAEIGKGVNIGCGTITVNYDGENKHLTKIEDDAFIGCNSNLIAPVTVGKGAYVAAGSTINQNVPEEALSIARARQTNKEDYAKKIKKPKKD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc)2. We require a protein that can specifically bind to the metabolites produced in the Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway.3. The protein must bind to Mg(2+) in order to perform its enzymatic function.4. The UDP-N-acetyl-alpha-D-glucosamine binding site should be located in a region of the protein that is accessible to the ligand.5. The protein have enhanced acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.6. A protein that can perform UDP-N-acetylglucosamine diphosphorylase activity in cytoplasm to support cell wall organization, UDP-N-acetylglucosamine biosynthetic process. The designed protein sequence is ",
    "response#1": "MKTVVILAAGKGTRMRSSLPKVLHPVAHKPMVGHVIDAARQVNAENIYLVYGFGGDVLKATLTKDETADDLTFVEQAEQLGTGHAVDMASPYLKDDEDVLVLYGDVPLTKVSTLEKLIAAKPESGLGRIVRDDATGAVTAIVEQKDATPAQRAITEINTGLLAAPTSDLKRWLAALDNDNAQGEYYLTDVIALAVADGVPVVATHPDDEIEVAGVNSPAQLARLERAHQARVADRLLEQGVTLVDPRRIDVRGKLACGRDVLIDINAVFEGEVELGDGVQIGANAVIRDTSIGAGTVIHPHCVLEQSRIGANCQIGPYARTRPGVEMADDAKLGNFVEIKKSRIGKGSKINHLSYIGDAIIGANVNVGAGTITCNYDGANKHRTTIGDGAFIGSGVELVAPVEIGAGATVGAGSTITKAVPADELVLERSQQVTIAGWRRPVKKSK",
    "response#2": "MKSLVLAAGAGTRMKSDTPKVLHTLAGRSMLSHSLHAVAKVAPQHLVVVVGRDRERVAPAALEIGQELGRPVEIAVQEQQQGTGHAAECGLSALPDDFTGVVVVTAGDVPLLDADTLADLIATHSAEAAVATVLTTTLADPTGYGRVLRTQDREVIGIVEQADASPSQQAISEVNAGVYAFDIAALRSALSRLRSDNAQHELYLTDVISIVRSDGDVLGVNERSQLAEAAGELAQAEATLQQRLRISAMEGGVTLVSPETTFFSTDTCLEPDVVVEPNVFFGPGVTVRSGALIRAFSHLEGCTVGEGAMIGPYARLRPGTICAADTHVGNFVELKNVDLGPGAKANHLTYLGDASVGAQTNVGAGTVTCNYDGYFKHRTTIGARNFIGSDAILVAPVTIGDDALIAAGSTITEDVPAGALAFGRARQHEQIVVGPRAAIDPGAELTGPCRILGATTIGQGATIGAFCQITNCVIDAGATVREFCHLEDAHVCAGAVAGPYSRLRPGADVGENARVGNFVEMKKASLGKGAKASHLTYLGDTAVGAGANIGAGTITCNYDGVHKHQTNIGAGAFIGSNTALVAPVQVGENALVAAGSVITKTVPDGALAVARARRVQLAAAEAEIQARLRRDAMLAGATLIAPETVFLSHDTMLGRDVTVEPHVVFGPGVVVGDGCVIHAFSHLEQARLEAGVQVGPYARLRPGARLAAGARIGNFVEVKNAAIGAGAKLNHLTYVGDAEVGAGANLGAGTITCNYDGVNKHRTTIGAGAFVGSNSSLVAPVTVGAGAYVGSGSVVTEDVPDGALALGRGRVK",
    "response#3": "MTIVLAAGMGTRRNSDVPKVLHKVAAAPLLHHALTAGASLSPDKVVVVTGHGAEEVARAARDYDEASDCVRQEPQLGTAHAVAQAAPHLADQPGEVIVLYGDTPFIRPETLEAMRAARAAHAVVVLGFHAKDPGRYGRLLTQGATLTAIREFKDATEPDAVAREIREVNAGVYLVDVPALRASLAHLGADNAQGERYLPDVIGLARSDGHRVAAHVTADEAMLEGVNDRVQLAELARRLNDRIVLAHMRAGVTLIDPSTTYIDAGVIVGEDTTLLPGVHILGESQIGGGCRIGPGAVLRDMRIGDRCEIGGSTLEGSSLADDVTVGPYSHVRPDSTIEAGVHLGNYAEVKASRVGARTQVGHFSYIGDSDVGCDVNIGAGTITANYDGVDKHRTVIGDGAFIGSDSVLVAPVTVGAKARTAAGAVVTHDVEAGSLVLGVPARPIAGWAARRRAGTTAAETSADGEQQ"
  },
  {
    "reference": "MPFSSYTGEKPLIYSLTQDELKEWLVGAGDKAFRAQQIFDWLYVKRVHSFDEMTNLSKDLRQKLNETFRIDPLREITRQVSQDGTVKFLFQLADGHAIETVIMRHNYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLDAGEIVAQVLMAQRKLDEEGERASHVVVMGIGEPFENFDNLIAFLRIINDNRGLNIGARHITVSTSGIVPKIYEFADKGGQVNLAISLHAPNTELRTKLMPINRGFPLEKLLEACRYYIAKTGRRISFEYGLFGGKNDQPEHAEELADLIGDMLCHVNLIPVNYVPERDYVRTPRNEIFRFKRILEERGINVTIRREHGSDIAAACGQLRAQYAKETAG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have a high affinity for [4Fe-4S] cluster, S-adenosyl-L-methionine.2. The designed protein must have a Proton acceptor, S-methylcysteine intermediate active site that is highly conserved among related enzymes.3. A protein that contributes to rRNA base methylation by functioning in cytoplasm with tRNA (adenine-C2-)-methyltransferase activity, metal ion binding, 4 iron, 4 sulfur cluster binding, tRNA binding.4. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.5. The stability of the protein-[4Fe-4S] cluster complex should be optimized to maximize enzymatic activity.6. The presence of Radical SAM core domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MTACISSQVGCSLTCAFCATGKLERLRNIDADEIFDQVALIKKQAEEHYKTPLTNIVYMGMGEPLLNYSNVLKSVELISSEEGLGMSPKRITVSTAGIAKMIMKLGDDQVKFNLALSLHAATDEQRSKIMPINETNSLEALGEALQYYHEKTGARVTFEYIIFKDFNDSLQDARNLAKFCKKVPCKINIIEYNPIDNAQFEKADSKKTDEFAQFLEQSWGNSVCVTTQVGCRMGCTFCASTVGGLVRNLTAGEIVDQIVMMQRELPQGERISTVVLMGSGEPLENYDHVLKAVRLVHDPEGLNIGYRHITISTSGIVPGMRRLAEEGLPITLALSLHAPTDELRRQLMPVARIWPLAEVLAAAREYGEKTGRRVTYEYILIEGVNDGPEEARQLARLLKGALAHVNLIPMNPVAERPQYRRPGPERVNRFKEILESNGIATTVRREMGGDD",
    "response#2": "MTACISSQVGCSLTCAFCATGKLERLRNIDADEIFDQVALIKKQAEEHYKTPLTNIVYMGMGEPLLNYSNVLKSVELISSEEGLGMSPKRITVSTAGIAKMIMKLGDDQVKFNLALSLHAATDEQRSKIMPINETNSLEALGEALQYYHEKTGARVTFEYIIFKDFNDSLQDARNLAKFCKKVPCKINIIEYNPIDNAQFEKADSKKTDEFAQFLEAESQEAAA",
    "response#3": "MTACISSQVGCSLTCAFCATGKLERLRNIDADEIFDQVALIKKQAEEHYKTPLTNIVYMGMGEPLLNYSNVLKSVELISSEEGLGMSPKRITVSTAGIAKMIMKLGDDQVKFNLALSLHAATDEQRSKIMPINETNSLEALGEALQYYHEKTGARVTFEYIIFKDFNDSLQDARNLAKFCKKVPCKINIIEYNPIDNAQFEKADSKKTDEFAQFLEYDKRKTICISSQVGCRMGCKFCASTKNGLVRNLTAGELIEEVYELERINGEINNIVIMGIGEPLDNYDNIVKFIDLITNADGRDLSHRSITLSTSGLAPMIRKLADSKLDVNLALSLHYANDKKRQKFMPVANKYKIEDLLEATDYYLAKTKRRVSFEYVVIDGVNNTDSDVDNLYSLLKNKNVHINLIPLNPIEEFAYNRPKSSALTEFRDKLVKRKLNATIRRSMGSELLSP"
  },
  {
    "reference": "MTPLDGLGVLTAAEMRSAEQAAMIGDVTEASLMDRAGAAIAQAVARLAAGREVLVLCGPGNNGGDGYIAAAELKRRGLAVRVAASEAPRSDGARRVAAFWPDAVEALERAEPAPVLVDAIFGTGLSRSPSAVVEHALARLTDAAWLRIAVDLPTGIATDTGKVLGKTPRFDVTLALGAVKPSHLLQPAASLMGQVRLLDIGVPTKSSARILARPILPQPDATSHKYNRGMVAIVAGSMSGASELAALAALRAGAGYVLHLASGDVHASAAPHAIVRRNYTADALNEDRIGAVVIGPGLGRDRDASDRLEAALASGRALVIDGDALRLVAVETLAMHDGPKILTPHGGEFTHLFGEVTDDKLSATRRAARQSGVVVVHKGADTVIAAPDGRAILCPEASPWLSTAGTGDVLAGAIGAMLAAGLEPLAAAEAGVWLHAEAARACGKAFIADDLADALSAVRG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein with ADP-dependent NAD(P)H-hydrate dehydratase activity, NADHX epimerase activity, ATP binding, metal ion binding functions is required to enable nicotinamide nucleotide metabolic process.2. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.3. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration4. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.5. The protein should have a high affinity for AMP, (6S)-NADPHX, K(+).6. The protein should be designed to have high stability and activity towards (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "MGVLDNNAEWLGIPKGYLMECAGYSFATEVYLRYNLSNKKNSHALIFCGTGNNGGDGFVIARHLASLGIESTVVLAGNPQKITTKEAKTSWNIISNHLTNTIRINIAKDSTDIVDLGIILDQTGYYNIIIDGLLGTGIKGKIREPIATAIDFINEAKTHKNIPIISIDVPSGLNPDTGEVSDKAVKADLVITFHKTKIGMKEGREFINEIVVRSIGIPQEAEAGPGDLLYLNKRKENSHKGQNGKLLIIGGGEYTGAPALTAQAALRAGADLTTIITTKTNKDIIAGYSPNLIVKGIDNYKKLTEINENKYDAAVIGPGIGEKHHEEINKHIKQTKLPTVLDADGINAIKNPKTLKNKIITPHNQEYKNLFNQKPTKQNIQKNAKKHRCIILKKGATDIITDGEQTKQNPAGTPAMTVGGTGDVLAGVTGALLSQNTKKPYRAAVAAAFITGKTGEHTAKKYGNGLLPTDIIKQIPQEIPKLEYITNKYTRGYTLVCSVGNKSIGASKLVAMSALRVGSGIVSIACDSNAFAFYASCLTSIMYRLYDDVINDDRITSIIIGPGCGISEITRQRTMDVLSKQNCVLDADSISVFSDSYEILFSKIKHNVIMTPHEGEFKRIFPFLTGSKIEMAQKAASLSKAVIVLKGPDTIIADPVGNVVVNNAPFSLATAGSGDVLSGIIGGLLSSGMNPFDAACCGVWIHTKCAREYGIGLIADDIILQIPQVLEIPKLEYITNKYTRGYTLVCSVGNKSIGASKLVAMSALRVGSGIVSIACDSNAFAFYASCLTSIMYRLYDDVINDDRITSIIIGPGCGISEITRQRTMDVLSKQNCVLDADSISVFSDSYEILFSKIKHNVIMTPHEGEFKRIFPFLTGSKIEMAQKAASLSKAVIVLKGPDTIIADPVGNVVVNNAPFSLATAGSGDVLSGIIGGLLSSGMNPFDAACCGVWIHTKCAREYGIGLIADDIILQIPQVL",
    "response#2": "MGVLDNNAEWLGIPKGYLMECAGYSFATEVYLRYNLSNKKNSHALIFCGTGNNGGDGFVIARHLASLGIESTVVLAGNPQKITTKEAKTSWNIISNHLTNTIRINIAKDSTDIVDLGIILDQTGYYNIIIDGLLGTGIKGKIREPIATAIDFINEAKTHKNIPIISIDVPSGLNPDTGEVSDKAVKADLVITFHKTKIGMKEGREFINEIVVRSIGIPQEAEGRGDIIPTLKLRRKDAHKGQYGRLLVIGGSKNYSGAPAYSSLTGIGFGIDLVITYTPEVIGDVLRSYSPNLIVRSSPGDWLNLDCFDEVSWLVDWANTIVIGPGLGQEKETENLLVELLKLFRKEEKNFVLDADALKLVKNHLELLSDQSVILTPHEGELKIMTDIELPSFDNLDERIKIISDISQNLNVTLLIKGPYDYISDTRNIKINKTGCPEMAIGGTGDVLAGLCGCFLSIGNSTFNSACSAAFLNGINGEYCKKTIGPRFTAMDMIKNINPSIEKLLKGDFGKLLVIGGSDKYHGAPILSAMSSIRAGVDLVIISTPELIANTIRAHTPNFIVRDYPGRYLTLDSVKYISDLINWADAIVLGPGLGVESETKEAVCKLFTLIKEANKPLVVDADALKVLAENPRILAGSPAVATPHRGEFKLLTGLEIKSSADILDNINEILSKISEFDITFAVKGAVDLVAYKNMFKLDLLGNPGMTVGGIGDVLTGVLGCFLSQGVEPFKAACAAVFITGLSGNLIAEKKGYHFTSAELIEYINAL",
    "response#3": "MLMENAGRAAYEEIKRRFNVNNSRIIVFCGTGNNGGDGFVVARQLSTHTIVDLVLLGLPERISRREAKINWEIVEKIYQRIRTHIISSTDDLQQIQSIVKSADIIVDAILGTGVKGPLREPLKTAIKMINEAKAKKISIDIPSGMDPGTGEIAGLAVKPDLTITFHKAKKAGPGDLLYLNKRKENSHKGQNGKLLIIGGGEYTGAPALTAQAALRAGADLTTIITTKTNKDIIAGYSPNLIVKGIDNYKKLTEINENKYDAAVIGPGIGEKHHEEINKHIKQTKLPTVLDADGINAIKNPKTLKNKIITPHNQEYKNLFNQKPTKQNIQKNAKKHRCIILKKGATDIITDGEQTKQNPAGTPAMTVGGTGDVLAGVTGALLSQNTKKPYRAAVAAAFITGKTGEHTAKKYGNGLLPTDIIKQIPQTQKKYEIPKLEYITNKYTRGYTLVCSVGNKSIGASKLVAMSALRVGSGIVSIACDSNAFAFYASCLTSIMYRLYDDVINDDRITSIIIGPGCGISEITRQRTMDVLSKQNCVLDADSISVFSDSYEILFSKIKHNVIMTPHEGEFKRIFPFLTGSKIEMAQKAASLSKAVIVLKGPDTIIADPVGNVVVNNAPFSLATAGSGDVLSGIIGGLLSSGMNPFDAACCGVWIHTKCAREYGIGLIADDIILQIPQVLTPTLWKNEIPKLEYITNKYTRGYTLVCSVGNKSIGASKLVAMSALRVGSGIVSIACDSNAFAFYASCLTSIMYRLYDDVINDDRITSIIIGPGCGISEITRQRTMDVLSKQNCVLDADSISVFSDSYEILFSKIKHNVIMTPHEGEFKRIFPFLTGSKIEMAQKAASLSKAVIVLKGPDTIIADPVGNVVVNNAPFSLATAGSGDVLSGIIGGLLSSGMNPFDAACCGVWIHTKCAREYGIGLIADDIILQIPQVLKKLF"
  },
  {
    "reference": "MSILSGKHVLLGITGGIAAYKSAFLVRLLKKAGANVKVIMTPDSEQFVTPLTLSTLSENTVISSFTDEEKNWNNHVELGLWADLMLIAPATANTLSKMAAGNANNLLITTYLSAKCPVYFAPAMDLDMYKHASSKASIEKLISFGNKCIPPESGHLASGLEGEGRMAEPENIVNFLEQDVLSQLPLYGKSITITAGPTYEAIDPVRFIGNHSSGQMGFELAEQALNLGATVNLITGPTHLKLAHKNLIRMDVISAQEMFEAATSIFDNSHIVIAAAAVSDYKPKVQFDQKLKKTEEALHIDLEPTPDILKHLGTLKSHQILVGFALETNNEEENAKAKLKKKNLDFIVLNSLNDKEAGFKKETNKIKIITNSEIIRFETKSKAEVANDILVHLLKFIS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must possess metal ion binding, phosphopantothenate--cysteine ligase activity to support pantothenate catabolic process, coenzyme A biosynthetic process under certain conditions.2. The presence of Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.3. The protein should have a fast turnover rate for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. A protein that can modulate the overall flux of metabolites through the coenzyme A biosynthesis pathway, enhancing or suppressing its activity as needed.5. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.6. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A. The designed protein sequence is ",
    "response#1": "MGYRALITSGPTREPIDAVRFIGNRSSGRQGAAIALELARRGMLVDYISGPSSIPTDHPGIRRVDVETALDMKAAAEAAMAHALPDAVIGTAAVADVRPATTISGKAAKTDLPDAISMVPNPDILAGINAVVSPECFKVGFALAHGNGQEAALRKLEAKNCDMIVLNSITDKGAGFEHTTNRVEWSGGVAMSGKHVLITLGPTREKWDAVRFWSNPSSGLMGACIAMAAWLRGAKVTVVSGPVNWWFPEDINVIKVNSAQQMFDAATEVWPSCTTGCFTAAVADFKPIPHGEGKFKKEGSSALRVDFDTNPDILKTIGSMKKDDQQLIGFAAETSNIQEAAKGKLERKNLDLIVANPINKPGAGFESSTNSVYSALERIGG",
    "response#2": "LGVGAGIAAYKACEVVRGLTESGHDVRVVPTRSALEFVGAATFEALSGNPVHSGVFSNVPEVEHVRIGQEADLVVVAPATADLLAKAAHGQADDLLTNTLLTARCPVAFFPAMHTEMWEHAATRSNVATLRSRGAVVPDPDSGRLTGVDSGKGRMADPAEILAAAAATVGGADLRGLKVVVSAGPTYEDIDPVRFIGNRSSGRMGFAVAEAAAQAGAEVTLVAGPVSIATPPGIARRIDVRSAAQMRDAVVTASAGADIYIAAAAVGDYRPAEVADHKLKKSDGAPLELRLTENPDILVTLAAQTVRPFLVGFAAETHDVERYAQDKLKRKGLDMIAANQVGGGLGFEAADNALTVVDAEGVETLPRAPKREIADRLLDTVLARMRR",
    "response#3": "MSGKHVLITLGPTREKWDAVRFWSNPSSGLMGACIAMAAWLRGAKVTVVSGPVNWWFPEDINVIKVNSAQQMFDAATEVWPSCTTGCFTAAVADFKPIPHGEGKFKKEGSSALRVDFDTNPDILKTIGSMKKDDQQLIGFAAETSNIQEAAKGKLERKNLDLIVANPINKPGAGFESSTNSVYGNSGIGAAINALELAGRRVLVTAGPTFEPIDPVRGITNLSSGKMGYAIARACRHAGAEVVLVSGPTALAAPAGVRRVDVRSANQMLAAVQGEIAGQDVFVAVAAVADWRVANASDRKLKKTDGGGPPALEFAQNPDILATVAALPGGPYCIGFAAETEDLEANAQAKRRRKGVPLLVGNIGQATFGLDDNELLLISAHGEEALPRLSKEETARRLVEHIAAIHA"
  },
  {
    "reference": "MISGSGSRTPSNRLYLSPVRPTMQNKRRLLREYRSYDDLSEHYRMFGSQSLDSLQDRVDMNPSGCDGLSTDGLDFCSQSHSGLLREHNFKIKSYYYNVGNCVHCRKRIRFAMASLRCRACPLRCHIGCCRQLTVNCIPQPQIGTKRGCLSDYAPRVAPMVPALIVHCVTEIEARGLQQEGLYRVSSTREKCKRLRRKLLRGKSTPHLGNKDTHTLCCCVKDFLRQLVHPLIPIYHRRDFEEATRHEDRLAVEMAVYLAVLELHQAHRDTLAYLMLHWQKIAESPAVRMTVNNLAVIFAPTLFGDLDLTLENVVTWQRVLKVLLLMPAGFWSQFLEVHPLPTSLGSTYDFEDRYNHRHWDSSSNLGWSSVKTYFRSMVNLSSTHL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that can perform small GTPase binding, guanyl-nucleotide exchange factor activity, metal ion binding, GTPase activator activity in centralspindlin complex, spindle midzone, midbody, cleavage furrow to support cell differentiation, mitotic spindle midzone assembly, mitotic cytokinesis.2. The protein must exhibit the following characteristics: Promotes the exchange of Rac- bound GDP by GTP.; GTPase-activating protein for p21-Rac; Involved in the morphogenesis of the adult appendages. The designed protein sequence is ",
    "response#1": "CDLTEYLESSGQDVPYVLKSCAEFIETHGIVDGIYRLSGITSNIQRLRQEFGSDQCPDLTREVYLQDIHCVGSLCKLYFRELPNPLLTYELYEKFTEAVSHRPEEGQLARIQNVILELPPPHYRTLEYLIRHLAHIASFSSKTNMHARNLALVWAPNLLRSKKIEATICNGDAAFLAVRVQQVVIEFILNHADQIK",
    "response#2": "CDLTEYLESSGQDVPYVLKSCAEFIETHGIVDGIYRLSGITSNIQRLRQEFGSDQCPDLTREVYLQDIHCVGSLCKLYFRELPNPLLTYELYEKFTEAVSHRPEEGQLARIQNVILELPPPHYRTLEYLIRHLAHIASFSSKTNMHARNLALVWAPNLLRSKKIEATICNGDAAFLAVRVQQVVIEFILNHADQII",
    "response#3": "TMSGYLYRSKGSKKPWKHLWFVIKNKVLYTYAASEDVAALESQPLLGFTVTLVKDENSESKVFQLLHKGMVFYVFKADDAHSTQRWIDAFQR"
  },
  {
    "reference": "MRRKLVAGNWKMNGSLAANTLLLTEIKSGLGNATSDVAVCVPSPYFAQCQGLLNGAAIALGAQDVSSHEAGAYTGEVSTSMLQEFDCRYVIVGHSERRAYFAETNELIAQKTVRALNAGLTPIVCVGETLDQREAGQTDSVVAEQLNVVLASIAASDLAKIVVAYEPVWAIGTGKTATPAMAQEVHAMLRKQLQAKNAEAAMQVRILYGGSMKPENAKELLAMSDIDGGLIGGAALKAADFLAIVHAA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The Electrophile of the protein must be engineered to enable effective catalysis of D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate.2. For general function, the protein need meet that Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D- glyceraldehyde-3-phosphate (G3P).; Involved in the gluconeogenesis3. The ideal protein would be one that plays a critical role in gluconeogenesis through triose-phosphate isomerase activity within cytoplasm.4. The protein design should take into account the physiological conditions of the glycolysis, Carbohydrate degradation, D-glyceraldehyde 3- phosphate from glycerone phosphate: step 1/1 pathway. The designed protein sequence is ",
    "response#1": "MITALIVAAGRGLRAGGGVPKQWRPLAGRRVADWTLAAFAGHPDVDQVVVCIHPEDRERADALAAGRPLRVVTGGASRAASVRAGLEALEATGADHVNVLMRVDFNVRPFVPAAVIDRLLAALDDAPGAAPALPVTDALWRGAEGRVTGTQDRTGLFRAQTPQAFRFDAILAAHRAWPGGAADDVEVARAAGLSVALVPGEERNLKITTPGDFARAEALMAGTSAPA",
    "response#2": "MTAILDVRNVLMRVDFNVGHERLDINTPEGRKQIASTLRHLVESGGAHVLTLSDHEGEPGDPLLHAHAQALSEELKREGKVGEIIPVNGFEEGGMIEEALKRRPDALILIPDMRHLSHDPGLTEELASRGIRVIGLGRRSHYPHQLLEIDIETLSY",
    "response#3": "MDKHIVFATNNKHKLEEVQAMLGASYTVKSLKDIGCHEDIVEDADTLEGNATQKAQYVYDNYHVNCFADDTGLEVEALDGEPGVYSARYAGPERSAEANMEKLLRELEGKENRKARFRTVIALIIDGKEYQFEGICKGEITDEKRGSQGFGYDPIFMPDGYDITFAEMEMSEKNRISHRALAVQKLVDFLNQKK"
  },
  {
    "reference": "MPKIKICGLKREEDVSFVNIAKPDYAGFVFAGVKRKIDFNTAVRFRSLLDDTIQSVGVFVNDKIDNIVNLCEDKTIDLVQLHGGEDESYIGRLKERIKKPVVKAVRVKDKIYSAATKADFMLFDTYSSFEYGGSGKIFDWDLLKEFRNPFFLAGGLNRDNIAEAVKKLNPYCVDLSSGVETNGVKDLNKIIETVKILRSL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be able to regulate tryptophan biosynthetic process.2. The protein design should consider the endogenous proteins and enzymes present in the L- tryptophan from chorismate: step 3/5, Amino-acid biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MRIKICGITRVEDVLFAEKAGADAIGVVMYSPTSRRSVPDAKAREIFSALGPFVTRVVVTHTESESDLEKILAIRPDAIQISHPFVFEKYPGVRVLRVIGRGDAVPTDCDAVIVDESMGAGKAFDQEFAKVVAKTSRVPVILAGGLTPENVGLAIREIRPHAVDVASGVETEPGIKDHTKIAAFIRAAREC",
    "response#2": "LDRIKAYKLEEVAAAKARVSFADLEAQARAQAPCRGFAEALRKKSAQGYGLIAEIKKASPSKGLIRPDFDPPALARAYEDGGAACLSVLTDTPSFQGAPDYLIAARNACALPALRKDFLYDPYQVAEARAWGADCILIIMASVEDALAVELEAAATGWGMDVLIEVHDGAELDRALKWLKSPLLGVNNRNLKTFEVTLDVTRQLAPVILAEGRELVCESGIFTPADMAAMAAVGARRFLIGESLMRQADVAAATRALYDFLKSNQNKLQIISEVKKASPSMGDINLDVDIVAQAKTYEENGAAMISVLTDEVFFKGDISYLKEISTQVAIPTLAKDFIIDEKQIVRSRNAGATVILLIVAALPEARLKELYDFATSLGLEVLVETHNLPELEVAHRIGAEIIGVNNRNLVTFETDINTSLELSTHFKDKPVYISESAIFTGQDAALVAPYFNGILVGTALMTADNVAKKVKEL",
    "response#3": "LDRIKAYKLEEVAAAKARVSFADLEAQARAQAPCRGFAEALRKKSAQGYGLIAEIKKASPSKGLIRPDFDPPALARAYEDGGAACLSVLTDTPSFQGAPDYLIAARNACALPALRKDFLYDPYQVAEARAWGADCILIIMASVEDALAVELEAAATGWGMDVLIEVHDGAELDRALKWLKSPLLGVNNRNLKTFEVTLDVTRQLAPVILAEGRELVCESGIFTPADMAAMAAVGARRFLIGESLMRQADVAAATRAKICGITRIEDALAAAEAGADAIGLVFYPKSPRAVTVLQARAIIAALPPFITTVGLFVNASRCELNETLDAVALDMLQFHGDETPEECDGYHRPYVKALRVKAGDDIAGVCRTYRNARGVLLDTYVEGVPGGTGETFDWALIPDDLDKPVILAGGLTSANVAQAIAQVRPYAVDVSGGVEKSKGIKDREKILAFMSAR"
  },
  {
    "reference": "MWLGVITLFPEMFRAITDFGVTGRAIKNGLLELHTWNPRDFTHDRHRTVDDRPYGGGPGMLMMVQPLKDAIQAARTAAGDGAKVIYLSPQGRKLTQRGATELAETQKLILVCGRYEGIDERIIQTEVDEEWSIGDYVLSGGELPAMTLIDAVSRLVPGVLGKQASAEQDSFSDGLLDCPHYTRPESLDGLEVPAVLLGGNHEDIRRWRLKQSLGRTFLRRPELFENLALTDEQTRLLAQFVDEMDSPQKS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be designed to catalyze the following reaction guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-   methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "GRAVKNGLLELQTWNPRDFTHDKHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKAATGDSAKVIYLSPQGRKLTQQGVEELVKSDSLILVCGRYEGIDERIIQTEVDEEWSIGDYVLSGGELPAMTLIDSVSRLVPGVLGKKASAEQDSFSDGLLDCPHYTRPETLDNLDVPAVLLSGNHEHIRRWRLQQSLGRTLLRRR",
    "response#2": "EVSEAGCIFRFDYSKVYWNSKLDTEHKRITSFFKPGEVVADVMAGIGPFAVPAGKKGVFVWANDKNPESHRYLEDAIQKNKVWEFVKPFNHDGHDFIRTSADLVLEASKRGDCAVIKPPRPPRKSAAPPPEPVRVPVPPTISHFVMNLPASAIEFLHNYRGLYHGHEDLFEPHTETKLPIVHVHCFSAKMDDDTPLKDICERIHAV",
    "response#3": "FNTVGHVAHLNIRDQYLPYKNIIAQVLLDKNPHIKTVINKIDNVGSENEFRTFAYEVLGGPDNMNVEVSEAGCIFRFDYSKVYWNSKLDTEHKRITSFFKPGEVVADVMAGIGPFAVPAGKKGVFVWANDKNPESHRYLEDAIQKNKVWEFVKPFNHDGHDFIRTSADLVLEASKRGDCAVIKPPRPPRKSAAPPPEPVRVPVPPTISHFVMNLPASAIEFLHNYRGLYHGHEDLFEPHTETKLPIVHVHCFSAKMDDDTPLKDICERIHKEIGVMLKPGDAEKEGEVLIYDVRDVAPAKRMFCASFRLPEG"
  },
  {
    "reference": "MNALTSLKLIDGKATAARVLAEVREQVQELRQGGVQPGLVVVLVGADAASQVYVRNKVLRAEEVGIRSQEHRLPADTTQAHLLTLIDRLNRDPEVNGILVQLPLPAHIDEHCVLQAINPLKDVDGFHSENVGGLAQGRDVLTPCTPSGCMRLLRDACGELRGKHAVVVGRSNIVGKPMAALLLQADCTVTVVHSRSRDLPALCRQADILVAAVGKPRLIGADCLKPGAVVIDVGINRINEDGQNHLVGDVDFAAALPQVAGITPVPGGVGPMTIAYLMKNTLLALDLQQQAAHQERTACLSPC",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein have enhanced 5,10-methenyltetrahydrofolate + H2O = (6S)-10-   formyltetrahydrofolate + H(+).2. The designed protein should be able to compete with endogenous proteins or inhibitors for NADP(+) binding.3. A protein with methenyltetrahydrofolate cyclohydrolase activity, methylenetetrahydrofolate dehydrogenase (NADP+) activity functions is required to enable methionine biosynthetic process, purine nucleotide biosynthetic process, histidine biosynthetic process, tetrahydrofolate interconversion.4. The protein design should consider the endogenous proteins and enzymes present in the One-carbon metabolism, tetrahydrofolate interconversion pathway to avoid interference with native metabolic processes.5. For general function, the protein need meet that Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate. The designed protein sequence is ",
    "response#1": "MSGKPLHEKTKEWARRHVRLLEEVGVTPKLAVLLLNDDPVELETQRRYVSLKARDVREVGGEVEIYELVDVPPERRSKEALYLIERLNRRDDVTGILIQKPVPPFVDEDLLFQRLSPEKDVRSMACGEGQSALVHPFKDVDGFTPFCLGRLLIDSPLFIPCTPKGIIRMLDEYKINLEGKQAVVIGRSIIVGKPLSLLLLKRNATVTMCHSKTINLQDITKKADILCVAIGREKFITSNMIKKGAVVIDIGINVTASGKVVGDVNFDDVKEKASYITPVPGGVGPMTIAMLMENAIYAAPAKDVDGFHPENVGKLVGGTPRFKPCTPHGVQKLLAHQGVDPDGAEVVVVGRSNIVGKPLANLLAQKAAGGNATVTICHSHTDDLAAHTRRADVVVAACGVPELIDGDMLSGDPVVIDVGINRVDADTEKGYELVGDVDYDSAEAAASAITPVPGGVGPMTRAMLLYNTLAAHPYNIGRLAQRIPALRPCTPKGIMTMIESTKRPVKGLDAVIVGASNIVGRPMSLELLLAGCTVTTCHKFTQDLKSHVQRADLLVVAVGKPNFIPGDWVKPGAIVIDVGINRVNDGSLVGDVDFDKAKERAGWITPVPGGVGPMTVASLIENTLEAYPQLA",
    "response#2": "MSGKPLHEKTKEWARRHVRLLEEVGVTPKLAVLLLNDDPVELETQRRYVSLKARDVREVGGEVEIYELVDVPPERRSKEALYLIERLNRRDDVTGILIQKPVPPFVDEDLLFQRLSPEKDVDGFTPGGVGPMTIDGKTIAATVRQAVKQQVKERIDSGLRAPGLAVVLVGSDAASEVYVGNKRRACEDVGFRSFDYDLPITTTQKELEKLIEELNDDDAVDGILVQLPLPAGLDATPILERIRPDKDVDGFHVVNAGRLAVGLDALVPCTPLGCVMLLKHHLGNLSGLNAVVVGRSNIVGKPAAQLLLREDCTVTIAHSRTRDLPGMCRQADILVAAVGRPEMVRGDWIKPGATVIDVGINRVPKADGKTRLVGDVAYEEALGVAGLITPVPGGVGPMTIACLLQNTLTAARRPDKDVDGFHPYNIGRLMQRKPTLRPCTPAGVITLLDSIGTPYKGQHAVIVGASNIVGRPMSMELLLKGATTTVCHRFTDNLEKFVGEADILVAAVGKPGIIKGEWVKPGATVIDVGINRMDDGKLCGDVDFDAAAERAAYITPVPGGVGPMTIATLLENTLYAG",
    "response#3": "MSGKPLHEKTKEWARRHVRLLEEVGVTPKLAVLLLNDDPVELETQRRYVSLKARDVREVGGEVEIYELVDVPPERRSKEALYLIERLNRRDDVTGILIQKPVPPFVDEDLLFQRLSPEKDVDPGGVGPMTSDHVVNAGRLAVGLDALVPCTPLGCVMLLKHHLGNLSGLNAVVVGRSNIVGKPAAQLLLREDCTVTIAHSRTRDLPGMCRQADILVAAVGRPEMVRGDWIKPGATVIDVGINRVPKADGKTRLVGDVAYEEALGVAGLITPVPGGVGPMTIACLLQNTLTAARRALFDLDRDLLPCTPAGILELLQMYGVEVKGRDVTVVGKGELVGKPLAVMLMQLDASVSVLHALTRDKLRYVKEADIVISAVGRPPELYRDNPWRLTGDMVKEGAVVVGVGGKVDPATGKWYFDVDEKSVAEKASYLTPNLGGVGLATRARLLKNLIRAE"
  },
  {
    "reference": "MDTTDRTAEVVLVDYGLGNLRSVTRGLERAGAEVTLSADPDDFSAADGIVLPGVGAFSEGMENAGPFREALVAAAADGQPLFGICLGMQMLLTSSEEAETVGQGDVRGLDLVPGRNVRFDEGQKVPHMGWNELNVARDHPIVEGIDGEYAYFVHSYYAAPDDDAAVVATTDYSVEFPAIVANEAGNVFGTQFHPEKSGETGLQILQNFVDYCLER",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must have at least one Glutamine amidotransferase type-1 domain.2. The designed protein must be optimized for lyase activity.3. For general function, the protein need meet that IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate; The resulting ammonia molecule is channeled to the active site of HisF. The designed protein sequence is ",
    "response#1": "LKILIIGKYINLKDSYKSLYEAIYHAGVQNKLKIKIEWLDSELINSKNAKNLLNEANGILIPGGFGNRGILGKIEAIKYARENNIPFLGICLGMQLAIIEYCRNVLKIKNVGSSEFGNFKNNVVSLMTEWSHKKIIVKRNKSSDMGASMRLGSYPCFILKNSLAHKVYKKLKINERHRHRYEVNPKLRKIIEKNDMKFSGFSKDKNLLEIVENSNHPWFLGVQFHPELKSKPFKPHPIFLSFIAANK",
    "response#2": "ESYQSLVEALKHASIHTLTQVEIEYIDSEAIEQNGLSALDALDAILVPGGFGARGVEGKIAAIQYAREAKIPYLGICLGMQLAVIEFARHVVGLAHANSTEFDPATPYPVVALITEWCDRAGAVEKRSAHSNLGGTMRLGAQRAPVKAGTRAARMYGASVNERHRHRFEVNGRFVPQLEAHGMIISAATPKDQLPEMVELTETLHPWFIGVQFHPEFASTPRAGHPLFNRFVG",
    "response#3": "LHDAYLSIVEALKHAGIRHGRAVDFLWLNAEDICAANAEEKLGEADGILVPGGFGDRGIEGKIETIRYARENQIPFFGICLGMQAAVIEFARNVAGLAGAHSSELSTDTPYPVIDIMPEQKDIDEKGGTMRLGAYPCKVKPGSKTYECYQSELIYERHRHRYEFNNEFREALEAAGLFIAGLSPDERLVEIVELPEHPYFVAVQFHPEFKSRPNRPHPLFAGFIG"
  },
  {
    "reference": "MSLPTGEGFGLNDNILETNVINLAVVVGVVVFFVGKNLTSILENRQETILNNLREADQRASEAREKFNKAKEQLELAEQKAKQIRSEGLLKATTEKNNCLTQYEQDLARLDEYKQETLQFYQQKVFSQLYVSLVSKALQKVKQKFDKRLDNQFHITVNNFFIARFTEYNP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that localizes to proton-transporting ATP synthase complex, coupling factor F(o) and can perform proton-transporting ATP synthase activity, rotational mechanism with high specificity and efficiency.2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "RGGKIGLFGGAGVGKTVLIMELINNVAKAHGGFSVFAGVGERTREGNDLYREMIESGVIKLGDKRGESKCTLVYGQMNEPPGARARVALTGLTVAEYFRDVEGQDVLLFVDNIFRFTQANSEVSALLGRIPSAVGYQPTLATDLGGLQERITTTTKGSITSVQAVYVPADDLTDPAPATTFAHLDACRRNLDKK",
    "response#2": "RGGKIGLFGGAGVGKTVLIMELINNVAKAHGGFSVFAGVGERTREGNDLYREMIESGVIKLGDKRGESKCTLVYGQMNEPPGARARVALTGLTVAEYFRDVEGQDVLLFVDNIFRFTQANSEVSALLGRIPSAVGYQPTLATDLGGLQERITTTTKGSITSVQAVYVPADDLTDPAPATTFAHLDAS",
    "response#3": "RGGKIGLFGGAGVGKTVLIMELINNVAKAHGGFSVFAGVGERTREGNDLYREMIESGVIKLGDKRGESKCTLVYGQMNEPPGARARVALTGLTVAEYFRDVEGQDVLLFVDNIFRFTQANSEVSALLGRIPSAVGYQPTLATDLGGLQERITTTTKGSITSVQAVYVPADDLTDPAPATTFAHLDAST"
  },
  {
    "reference": "MDLKNLIKELEVESIYGPIDVQIDNIHYDSRLIKPGGLFICIEGFMTDGHNYINEAIENGAVAIAVEKEIHEDRVPIIRFKNTRRAMASIASNFYGKPSQLLDMIGVTGTNGKTSVTYIIKSILENYDRKTAIIGTLATLINNEKMYSARTTPESLDLQGLFRKMIDGDVDTCIMEVSSHSLELNRVDEVSFKVGIFTNLTPDHLDFHSTMDNYREAKKKLFYKTTHCNIINLDDEAGRIIVKDIKNLDTPLLTYSINNPADFVAENIHNNGKELSFDVVGPENFNIKLTLKTPALFAVYNALASIACCYSLGVPADIIADGLNAFIGVPGRFQSIDGLKEFSAVVDYAHTPDALENVLKAAKEFTKNKLITVFGCGGDRDQTKRPVMGEIAGKYSDFSIITSDNPRSEDPYVILSMIEEGIKRTKGNYIIIEDRKEAIREAVKMAEAGDLIVIAGKGHETTQIVKDRVLVFDDKQVVLEVAGEEGRI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a binding site that is specific to the target ATP, meso-2,6-diaminoheptanedioate, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate ligand.2. The presence of Mg(2+) is necessary for the protein to function in the desired environment.3. A protein that is capable of catalyzing reactions within the peptidoglycan biosynthesis pathway with high efficiency and specificity.4. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..5. The protein should have a fast turnover rate for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.6. The protein contains novel Mur ligase N-terminal catalytic, Mur ligase C-terminal, Mur ligase central domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MRLKELINFIPYQILGNSDVEISGIEFDSRKVEVGSLFVAVKGYKADGHDYIDKAVASGASAVICENIPENTMIEKIVSSDSQRAFAIISANFYQKPSNKLKLVGITGTNGKTTTAYILKSMLEKSLFKCGFISTVAYSTGESYIEGSRTTPEAWELQKLFDGMRKNSCQKVVMEVSSHGLDQTRTASARFKAAVFTNLTGDHLDYHRDMENYFNAKKRLFTEFLHDNGKAVINIDDPYSQKIVQLLPPEKVISLGTSSSADCRIFLRSASAAGSDCEFLFREKTIHFRSNLIGIHNMYNLASAFLAVGCLQGISLNQAIESLSVLEGVKGRMNRLGGAGRPTVVVDYAHTPDALEQALASLRDHVRGRLLCVFGCGGERDRGKRAQMAAIAERLADAVVVTDDNPRGEDGDAIVAAILEGFANQDTVTVQRDRAAAIAHAIGAAGPGDIVLVAGKGHETYQIIGQERIPFDDSKVVRECLQN",
    "response#2": "MKLKDLSFEITGVEYDSRRVREGSLFVAMRGGTTDGNKFIRQAMDKGAGGVLTDSRLSAMPISGIEFDSRKVEVGSLFVAVKGYKADGHDYIDKAVASGASAVICEVTGTNGKTTTVTLLYSLFKRMGYKTGLLSTVVNRIHNETVTATHTTPNAIELNALLREMVDAGCMYCFMEVSSHAVHQKRIAGVRFAGAVFTNITHDHLDYHGTFDEYLKAKKGFFDMLSADAFALVNKDDYHGEEMVKDTKARVRTYAIKSMADFRARVVENQFSGLQLNIDGKDLWTKLIGSFNAYNILAVYTLMAYFGLSSEKIIQSLPKIKVPGRLEQVETGRDFHVVVDYAHKPDAVEAALSTLRPLTAQAGGRLICVIGAGGDRDTGKRPVMGEIAARLADLVIVTDDNPRSEDPAVIRAAVLEGTRDGAGEVREVGDRREAIRVALSSAGPGDIVLVAGKGHENYQEIKGVKYPFDDKIVAKEALLNR",
    "response#3": "MNVTGITHDSRAVRPGDVYVAFPGANFHGARFAAQAVAAGAVAVLTDAAGAELAADCGAPLLVVDSPRARMGELAAALLAVTGTSGKTSTAWFTQGLFAATGHTAGYLGTLGLHVPGLPEQYTLTTPDPVALHLYLAQAADAGVTHFALEASSHGIDQRRLDGVRLKAAAFTNLQRDHLDYHATMAAYFEAKARLFLDLLPENGTAVVHAGDDWGQVLRDRVQQRGQTCLTYGTKTADLALTGRALAPGGQALTISALGETAEVIFPVLGSFQAENLLAALGVLLELGFGYAAVLQALGRLQPVAGRFQTVPNDRDITVAVDYAHTDDALLNLIRLARELAGKEHRVITLFGCGGDRDRTKRPKMGRAAGRGSDLVVLTSDNPRSEDPGIILEQALRGVEETGTDCIVEADREQAIRRALAGAKPGDIVLLAGKGRESWQEVGEAALAAL"
  },
  {
    "reference": "MADPLVQSGKRKREEESESTQTPGPRGDKAAVGNGTAASTSFPFSGFRLQKVLKESSRDKIIFLHGKVNETSGDGDGDDAVVILEKTPFQVDQVAQLLLGSPELQLQFSNDIYSTYNLFPPRPLSDVKTTVVYPATEKHLQKYLRQDLHLIRETGEDYKNITLPYLDSQSLSIQWVYNILDKKAEADRIVFENPDHSDGFVLIPDLKWNQKQLDDLYLIAICHRRGIKSLRELTGEHLPLLRNILQEGQEAIQQRYQVREDRLRVYLHYQPSYYHLHVHFTALGFEAPGSGVERAHLLADVIENLEYDPQYYQKRTLTFALRADEPLLKLFQEAKKN",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that can perform identical protein binding, m7G(5')pppN diphosphatase activity, RNA 7-methylguanosine cap binding in cytosol to support negative regulation of programmed cell death.2. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile to enable effective catalysis of a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside   in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + 2 H(+) +   N(7)-methyl-GMP.3. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "MNSVDFRLKIVKEEGAGIVSRKVGHLYVGHLDKPFARMKYTKSLKDLDNKIIECTFEDNQWKFMRERTDKSFPNSFNTAKGVCGSISNPITKEKLL",
    "response#2": "MNSVDFRLKIVKEEGAGIVSRKVGHLYVGHLDKPFARMKYTKSLKDLDNKIIECTFEDNQWKFMRERTDKSFPNSFNTAKGVCGSISNPITKEKLL",
    "response#3": "LCKTKKIKFGLWIDLTNTTRFYDKEEVEDEGCKYIKLQCRGHGETPSKEQTNTFIQLVHNFITHHPLEKIAVHCTHGFNRTGFLIVSYLVEKMDMELELAIETFAKMRPPGIYKGDYLTELL"
  },
  {
    "reference": "MKVLKVIPIKKPLDAEVAIPGSKSYTNRALLMAALSGNTVRIKNPLISEDTEAMIGCLRELGIACTLEDGLVTVAGGIDLVSDREYNLDANLSGTTIRFISALSALVPGTKTIHGKPRLNERPIYDLVDALRQLGASIEYLDREGYPPIRISSSELSAGVVKMKGEVSSQFLSALLMVAPRIGDVEIHIEGEQISKPYVDMTIDAMKQCGVTVVNDQYAKYRITAGAKYKMTEYVVEGDVSSASYFAAIAALTGSRITLKNMNPQSVQADMGFLTILAGMGNEITNGENEITIQGRGVRAVDVDMRGCPDQVQTLAVLSAFVSGITSISGVRSLRVKETERVAALERELAKMGVQAESTPDTLTIHGRAPRPAVIDTYWDHRMAMAFTVAGTKLEGMEIRNPDVVGKTFPDFWEKLNSIGIKTEISEREPNIVLIGMRGSGKTTIAKMLSTRLQRTSIEIDDEVVKKTGMSIADIFAHHGAEYFRNKESEVVDEMLAKENTIISTGGGTILRPHNIEQLKYNAICFWLRAPVEALVERMESDPSSSQQRPTLTDKETPEEETAEVLRQREALYQKAADEVVDTQNKNPGGIVEEVLLKLKARGINVQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein have Enolpyruvate transferase domains with specific sequence motifs that are crucial for its activity.2. The protein design should consider the endogenous proteins and enzymes present in the chorismate biosynthesis pathway to avoid interference with native metabolic processes.3. For general function, the protein need meet that Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.4. A protein with 3-phosphoshikimate 1-carboxyvinyltransferase activity functions is required to enable chorismate biosynthetic process, aromatic amino acid family biosynthetic process, phosphorylation.5. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP, Mg(2+) binding. The designed protein sequence is ",
    "response#1": "MGYSPEQLSGRLSVPGDKSISHRSMILAAISDGPCQIVGFLQSADCLATMQIMQALGVTIEVCDGGYVVNGVGLHGLKKPAEDLDCGNAGTAMRILAGLLAAQPFATTLVSDESLSQRPMRRIVEPMTQMGASIESDQNTAPLSFKPVQGLTAIHYHSPVASAQVKSAILLAGLYADGPTTVTEPQLSRDHTETMLQGFGCQIKRDGLSVSLTPPDNLIAQDIHVPGDVSSAAFFMVLGLLHPQADFVIEQVSINPTRAAVIDILKMMGGHIEIFNQSEQGGEPVACLRIKSSQLQGIDIPQHLVPSAIDEFPILFIAAALAKGTTRLSGAEELRHKESDRIAVMCHGLQLLGIQCTEQADGAVIQGGRLSSGVVDGKGDHRCAMSFLMASALTDQAISVRGCHNINTSFPQFFDVLNGLKT",
    "response#2": "MTKITAPASKSHAQRAILASAWAKGKSVLYGYTPCADSDAALNVIRTLGAKVKIEKCKLPKFRIEIVSPGIEELAKKLAKEEIFSKNIKPVELNVGESGLLCRLMIPTSGHLPGKSKGVDEITIKGEGGLNHRELFVSDEPLRELGMEIKTQEGKLPAVISGGLKGTSLKISGKGGSQLLSGLLMSLPMCEKSSVIELSEPTSIPYIDLTIKTIKDFGINVTTTDYREFRIPGKQRYKAKTFFPIEGDWSGASMILVAGAIKNGVTVTNLPVGSKQADEKILDILRSCGAEINITEYPKYLVMCGDEPCVFKEDDKKEIILGSQIDIPKPKNPLLPFEFDATNAPDLFPTLVVLALNCAGTSKIKGVQRLSNKESNRAESLLCEFTKLGAHIDIEGDWMIVEGGRLHGGYCSSHNDHRIAMSIITASLNIDQKVYVDDLNCISKSFPDFIKKLESL",
    "response#3": "MNICIDGPTASGKGTLASQVAQRLGYHYLDSGALYRITAFAARRAGLELDVAHEHAVAKLAQALAIRFDGTQVFLDGEDVSEAIRTEQAGMDASRVSALPAVRTALVALQQGFRRLPGLVADGRDMGTVIFPDAPLKVYLTASAAQRAERRHKQLISKGIPATIDVLRADLEARDARDSSRAVAPLKPAEDALLLDNSSQTIEQSVAQVLAALRP"
  },
  {
    "reference": "MLRLVSWNINGIRSPLQGVRCEEPSSCSAMAMGRILDKLDADIVCLQETKVTRDVLTEPLAIIEGYNSYFSFSRNRSGYSGVATFCKDSATPVAAEEGLSGLLSTQNGDVGCYGNMDDFTQEELRALDSEGRALLTQHKICTWEGKEKTLTLINVYCPHADPGKPERLTFKMRFYRLLQIRAEALLAAGSHVIILGDLNTAHRPIDHWDAVNMECFEEDPGRKWMDGLLSNLGCESGSHMGPFIDSYRCFQPKQKGAFTCWSTVSGARHLNYGSRLDYVLGDRTLVIDTFQSSFLLPEVMGSDHCPVGAVLSVSSVPAKQCPPLCTCFLPEFAGTQLKILRFLVHFKQDPVFKQSALQPSNQTQVHMRKNKARVRSTRSRPSKTGSSRGQKNLMSYFQPSSSGPQTSNLDLPSLGTLITPKTSEEDVMANVVEGQTKASEAKDEKEIRTSFWKSLLGGPSPMPLCGGHREPCVMRTVKKPGPNLGRHFYMCARPQGPPTDPSSRCNFFLWSRPS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Mg(2+), Zn(2+) binding site should be stable and able to withstand changes in environmental conditions.2. The protein must be engineered to have a high affinity for Mn(2+), Mg(2+) in order to increase its catalytic activity.3. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.4. The ideal protein would be one that plays a critical role in DNA recombination through zinc ion binding, exonuclease activity, DNA binding within nucleus, mitochondrion.5. For general function, the protein need meet that Functions as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents; Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially; Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes; Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends; Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents; Required for proper cell cycle progression during proliferation of peripheral lymphocytes . The designed protein sequence is ",
    "response#1": "RKVAIDASMCLYQFLIAIRQDGSQMQSEDGETTSHLMGMFYRTIRMIDNGIKPVYVFDGKPPDMKSGELEKRTERRAEAEKALTEAKEKGDAKEAEKFERRLVKVTKQQNEEVKQLLGLMGIPVVEAPCEAEAQCANLVKAGKVYGTATEDMDALTFGSCVLLRHLLAPEAKKIPIKEFHLARVLEEMQLTKDQFIDLCILLGCDYCGTIRGIGPKKAVELIKQHKNIETVLENIDQTKYPPPADWPYKRARELFHEPEVMKCDEVELTWKDPDVTSFLDPPVSPPGIPVVEAPCEAEAQCANLVKAGKVYGTATEDMDALTFGSCVLLRHLLAPEAKKIPIKEFHLARVLEEMQLTKDQFIDLCILLGCVIKPAYVFDGEPPAFPELPEVEVISRGLAESLEGKTIESVKILNHSSVKMPWYLFSSRVAGEKITRIHRRAKLLIMDLGDDLHITFHLKMTGRVLAHEGPTTPEPHTRIVFGLTDGGSIEFHDTRKFPELPEVEVISRGLAESLEGKTIESVKILNHSSVKMPWYLFSSRVAGEKITRIHRRAKLLIMDLGDDLHITFHLKMTGRVLAHEGPTTPEPHTRIVFGLTDGGSIEFHDTRKFT",
    "response#2": "MGIKGLSQVIADHAPSAIKVQEIKAFFGRKVAIDASMCLYQFLIAIRQDGSQMQSEDGETTSHLMGMFYRTIRMIDNGIKPVYVFDGKPPDMKSGELEKRTERRAERMRSASASVMREETSPELPEVEVISRGLAESLEGKTIESVKILNHSSVKMPWYLFSSRVAGEKITRIHRRAKLLIMDLGDDLHITFHLKMTGRVLAHEGPTTPEPHTRIVFGLTDGGSIEFHDTRKFGEVSGYMCSCHGVPVVQAPSEGEAQAAYMAAKKRVYASASQDYDSLLFGAPRLVRNLTITGRRKLPGKNVYVEVKPELIVLEEVLRELGIDREKLIELAILVGTGMADIVCLQETPELPEVEVVRRGLEEHMVGRTIVSAAVVHPRTARNQAGGGAEIEANLTGLRVGATNRRGKFLWLELDDVAQQAPSGLGLLVHLGMSGQMLVKSPDATLNPHLRARAELDDGNEVWFVDQRTFGYWRDGLNVPELPEVEVVRRGLEEHMVGRTIVSAAVVHPRTARNQAGGGAEIEANLTGLRVGATNRRGKFLWLELDDVAQQAPSGLGLLVHLGMSGQMLVKSPDATLNPHLRARAELDDGNEVWFVDQRTFGYWRDGVNVEGRGVSWNINGIRSPLQGLACQEPSSCPTALRRVLDELDADIVCLQETKVTRDVLTEPLAIVEGYNSYFSFSRSRSGYSGVATFCKDSATPVAAEEGLSGVFATLNGDIGCYGNMDEFTQEELRVLDSEGRALLTQHKIRTLEGKEKTLTLINVYCPHADPGKPERLTFKMRFYRLLQMRAEALLAAGSHVIILGDLNTAHRPIDHCDASSLECFEEDPGRKWMDGLLSNPGDEAGPHIGLFMDSYRYLHPKQQRAFTCWSVVSGARHLNYGSRLDYVLGDRALVIDTFQASFLLPEVMGSD",
    "response#3": "MGIKGLNSIITEHVPSAIRKSDIKAFFGRKVAIDASMSLYQFLIAVRQQDGGQLSTETGETTSHLMGMFYRTLRMIDNGIKPCYVFDGKPPVLKSHELDKRTSRREPELPEVEVISRGLAESLEGKTIESVKILNHSSVKMPWYLFSSRVAGEKITRIHRRAKLLIMDLGDDLHITFHLKMTGRVLAHEGPTTPEPHTRIVFGLTDGGSIEFHDTRKFSYIHYLPELPEVEVISRGLAESLEGKTIESVKILNHSSVKMPWYLFSSRVAGEKITRIHRRAKLLIMDLGDDLHITFHLKMTGRVLAHEGPTTPEPHTRIVFGLTDGGSIEFHDTRKFSYLHYTDLGMERNSPELPEVEVISRGLAESLEGKTIESVKILNHSSVKMPWYLFSSRVAGEKITRIHRRAKLLIMDLGDDLHITFHLKMTGRVLAHEGPTTPEPHTRIVFGLTDGGSIEFHDTRKFSYIHYLDLGMERNSRAKPELPEVEVISRGLAESLEGKTIESVKILNHSSVKMPWYLFSSRVAGEKITRIHRRAKLLIMDLGDDLHITFHLKMTGRVLAHEGPTTPEPHTRIVFGLTDGGSIEFHDTRKFSYIHYLDLGMERNSRAKYSYIHYLDLGMERNSRAKYSYIHYLDLGMERNSRAKYSYIHYLDLGMERNSRAKYSYIHYLDLGMERNSRAKYSYIHYLDLGMERNSRAKYSYIHYLDLGME"
  },
  {
    "reference": "MARTLAEKVWDDHVVRKGQDGEPDLLYIDLHLVHEVTSPQAFDGLRAAGRPVRRLDLTIATEDHNTPTVDIDRPLSLLEDKTSAKQLQTLRENCAEFGVRLHPLGDAEQGIVHVVGPQLGLTQPGMTVVCGDSHTSTHGAFGSLGMGIGTSEVEHVLATQTLPLKPFKTMAITVDGTLKPGTTAKDVILAVIAEIGTGGGQGYVLEYRGEAIRNLSMEGRMTICNMSIEAGARAGMVAPDETTFAYLEGRPHAPQGADWDAAVEYWRTLRTDDDAEFDAEVVLDGSALEPFVTWGTNPGQGVPLSGSVPEPERIGDDGVRQGVERALEYMGLEGGTPMRDIAVDTVFIGSCTNSRIEDLRAAADVVRGRRKADSVRVMVVPGSAKVRLQAEAEGIDTVFKEFGADWRFAGCSMCLGMNPDQLAPGERCASTSNRNFEGRQGKGGRTHLVSPLVAAATAVRGTLSSPADLEPPVPTNV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. [4Fe-4S] cluster is required for the proper folding and stability of the protein.2. For general function, the protein need meet that Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate.3. A protein with 4 iron, 4 sulfur cluster binding, 3-isopropylmalate dehydratase activity, metal ion binding functions is required to enable leucine biosynthetic process.4. The designed protein should be able to compete with endogenous proteins or inhibitors for [4Fe-4S] cluster binding.5. We need a protein that can perform (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate with high efficiency.6. A protein should be able to manipulate the activity of enzymes in the Amino-acid biosynthesis, L-leucine biosynthesis, L-leucine from 3-methyl-2-oxobutanoate: step 2/4 pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "RHLVHEVTSPQAFEGLRNANRKVRRTDCTLATVDHNIPTASRKSYRDTKSFVEQVDSRTQCMTLEENVKAFGLTFFGLSDNRQGIVHIIGPEQGFTLPGATIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLLQKRAKNMLIQVDGELSQGVTSKDIILHIIGLIGTAGGTGHVIEYAGSTIRSLSMEARMSICNMSIEAGARAGLIAPDEITYEYIKGRPLAPKQGEAWDQALAYWKTLPSDEGAQYDTVIKIDAKDIPPTVTWGTSPQDVVAITGTVPDPKNASNEAEAKAWTRALEYMGLEAGTPMEKIKIDKVFIGSCTNARIEDLRAAARVLHGRKVADGLYCMLVPGSGLVKKQAEAEGLDKIFQAAGFDWREAGCSMCLGMNPDQLAPGERCASTSNRNFEGRQGAGGRTHLMSPAMAAACAVTGYGHFAGDFVLANIDLAMTHDITGPLAVQGFYEIMRDEEDKKVWDPSKIVIIFDHQVPADSINAAQNHIMLRKFAKEQGILNYDVYEGVCHQVLPEKGHVKPGDLIVGSDSHTCAYGSLGAFSTGIGSTDMAAVFATGKLWFRVPETFRFEVEGKLPERVYSKDLILHLIGDVGVEGVRYMAAEYAGSTIRSLSIPERMTMSNMAIEMGGKAGIIEADEVTEAYLKERIPGFKLDPYWKSDEGAKYLDIRYYDVSDLEPQVACPHNVDNVKPVSVHVIGPELGFEWREPGCSMCLE",
    "response#2": "MEKFTTLTGIAAPLERSNVDTDLIIPKQFLKTIKRTGLGSALFWPLRYDAKTGEPDPAFVLNQKPYDQSKILVVTGPNFGCGSSREHAAWSLLDFGIRAVIAESFGDIFRNNLTKNGQLPVVLSPETREKIDTDNWLERSNVDTDLIIPKQFLKTIKRTGLGSALFWPLRYDAKTGEPDPAFVLNQKPYDQSKILVVTGPNFGCGSSREHAAWSLLDFGIRAVIAESFGDIFRNNLTKNGQLPVVLSRAQIQRLTQDAKAGKQITVDLVEYDDYPGDADKVWDLHRIADLPGGATQLFVGLHLIHEVTSPQAFAALEEKGLSVNCPDRTIATVDHIVPTTNHQRPFSDPLAEEMLHTLEKNCSNHHIKLFGIGSGNQGIVHVMAPESGLTQPGMTIACGDSHTSTHGAFGAIAFGIGTSQVRDVLATQSLAMKKLKVRRIWVDGQLTNGVFAKDLILHVIRHLGVKGGVGYAYEFAGPAIKKLSMEERMTICNMAIEGGARCGYVNPDQTTFDYLEGKPYIPTGQEWESALQWWKELASDQNAIFDDEVKFDACKISPTVTWGITPGQAIGIDELIPKVDSLETSDQQTAREAYLYMNLHPGSSIEGLGIDVCFIGSCTNGRLSDLQAAAKIVKNRHVAKGIKAFVVPGSEKVAKAAEAEGLDVLFQNAGFEWRKPGCSMCLAMNPDRLEGNQISASSSNRNFKGRQGSARGRTLLMSPAMVAAAAISGKVTKV",
    "response#3": "RHLVHEVTSPQAFEGLRNANRKVRRTDCTLATVDHNIPTASRKSYRDTKSFVEQVDSRTQCMTLEENVKAFGLTFFGLSDNRQGIVHIIGPEQGFTLPGATIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLLQKRAKNMLIQVDGELSQGVTSKDIILHIIGLIGTAGGTGHVIEYAGSTIRSLSMEARMSICNMSIEAGARAGLIAPDEITYEYIKGRPLAPKQGEAWDQALAYWKTLPSDEGAQYDTVIKIDAKDIPPTVTWGTSPQDVVAITGTVPDPKNASNEAEAKAWTRALEYMGLEAGTPMEKIKIDKVFIGSCTNARIEDLRAAARVLHGRKVADGLYCMLVPGSGLVKKQAEAEGLDKIFQAAGFDWREAGCSMCLGMNPDQLAPGERCASTSNRNFEGRQGAGGRTHLMSPAMAAACAVTGYGHFGCEWREPGCSACLYS"
  },
  {
    "reference": "MPLSRLIIQQFRNIKACDIALSPGFNFLIGPNGSGKTSVLEAIYLLGHGRSFKSALTGRVIQNECDQLFVHGRFLNSDQFELPIGINKQRDGTTEVKIGGQSGQKLAQLAQVLPLQLIHPEGFDLLTDGPKHRRAFIDWGVFHTEPAFYDAWGRFKRLNKQRNALLKSAKSYQELSYWDKEMARLAELISQWRADYVAQMQSKAEQLCQEFLPEFHIQLKYYRGWEKETPYQQILEENFERDQTLGYTVSGPNKADLRIKVNNTPVEDVLSRGQLKLMVCALRLAQGQHLTEKTGKQCVYLIDDFASELDSQRRKRLADCLKQTGAQVFVSSITENQISDMRDDSGRLFNVEQGVIEQG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that It also seems to bind ATP.2. The designed protein should be optimized for ATP binding activity in order to improve DNA repair. The designed protein sequence is ",
    "response#1": "IVTGPNMSGKSTYIRQVGLIAVMAQIGSFVPADDAEIPIFDRVFTRMGARDDISTGKSTFLIEMSEVALILEKATKKSLVLLDEVGRGTSTFDGISIAWAMSEYIYNEIGCETMFATHFTELTELSDVYEGIKNLTIEVRETNNGVVFLHKVVEGIADRSYGIEVAQIAGVPDGVH",
    "response#2": "IVTGPNMSGKSTYIRQVGLIAVMAQIGSFVPADDAEIPIFDRVFTRMGARDDISTGKSTFLIEMSEVALILEKATKKSLVLLDEVGRGTSTFDGISIAWAMSEYIYNEIGCETMFATHFTELTELSDVYEGIKNLTIEVRETNNGVVFLHKVVEGIADRSYGIEVAQIAGVPDGVN",
    "response#3": "MVITGPNMAGKSTVMRQVALTALMAQAGSFVPAKAARIGLCDRIFTRVGAADNLARGQSTFMVEMTETSHILHHATNKSLIILDEIGRGTSTFDGLSIAWAVAEHLHDTVGARALFATHYHELVDLARERPRVKNLCVAVI"
  },
  {
    "reference": "MANVLVAMSGGVDSSLAAALLLEAGHQVTGVTMHLWDDDEQGLRESLCCAAEAAASARRVCALLGIPFYVFNYQREFRRHVIDYFIRAYTHGLTPNPCVECNRMIKFRALLDRARTLGFDAVATGHYARIIQGEDGRYQLWRAVDLEKDQSYMLHMLGQAELSRLIFPIGAYTKREVREMAAARGLPSADREESQDICFVPDGDYRNLLRIESPESLVPGPIVDLEGREIGRHRGLPLYTVGQRRGLGLGGGEPRYVVAIDPARNALIVGPAAALNRARFTVIDACWVDDAPPAESFTCLVQVRAHAEPLPARVSQQPDGRWLVELERPQRAVSPGQAAVFYRGQQVLGGGWIARPEVA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be optimized for sulfurtransferase activity. The designed protein sequence is ",
    "response#1": "MEKYGYKVEDFDYAVFHMPNAKFPVRAAKMLGFSMEHISQGLVVKAIGNTYSGSSLLGLAATLDVAEPDERILLVSFGSGAGSDAFAIY",
    "response#2": "MEKYGYKVEDFDYAVFHMPNAKFPVRAAKMLGFSMEHISQGLVVKAIGNTYSGSSLLGLAATLDVAEPDERILLVSFGSGAGSDAFAIL",
    "response#3": "WRELTKPSRLRLVAMAMADMIVSEVKEDIEVVVGIATSGIPLAAMVAEELGCEFTIYYPRKFKTDEEKPKGGILSENFARVSGKKCAIVDDITSTGRSIKEAIEVIEANDGKAVCAAVIVNKRGGR"
  },
  {
    "reference": "MSLIIQSLPHWSRIPPRPPQLSQFQNSSRPKPLIQAGQVQHNALQIARRSANYHPSIWDPQYIESLKSPYGDECFGTRLEKLKFEAKRLLEATIEPLSWLELVDSIQRLGVAYHFEDEIKEGLDGVYGVGAHAGDDLYTAALQFRLLRQHGYGVTPDIFNKFLEKERTFKACTSLDAKGLLSLYEASHTMIHGEEVLEDAKEFSVKHLNYLMGNLQNNLREQVQHALEMPLHWRMPRLEAKHYIDVNGRSDERNMVLLELARLDFNFVQSKHQEELKEVSRWWRDLGLAKKLGFSRDRLVENYLWAVGIAPEPKFSNCRKGLTKLISILTVIDDIYDVYGSLDELELFTEAVKRWDIEALETLPEYMKICYLALFNFVHEVSYDTLKDYGWNILPFIREEWERLCMSYLVEAEWFGNGNKPALDEYLRNGWISVGGPVAMVHAYFLQGRPIRKDSINFLDHGSELIYWSSVATRLNDDLGTSKAEMKRGDVPKAVECYMIQTGESYEDAREHIQGLVRDCWKKMNEECLKCCLPKSYVETVLNMVRTAQCIYQHGDGIGTSTGVTQDRVISLICEPVPSQWP",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.2. The protein design should take into account the physiological conditions of the Secondary metabolite biosynthesis pathway.3. The protein should be able to bind Mg(2+) ligand in a variety of conditions4. For general function, the protein need meet that Monoterpene synthase (TPS) involved in the biosynthesis of monoterpene natural products used by traditional Chinese medicine to treat headache, inflammation and intoxication (Ref.1)5. A protein with magnesium ion binding is needed to enhance green leaf volatile biosynthetic process, diterpenoid biosynthetic process in a specific context.6. The protein should have a low Km value for (2E)-geranyl diphosphate = (E)-beta-ocimene + diphosphate. The designed protein sequence is ",
    "response#1": "MNPILLEFAKLDFNMVQATHQEDLRHMSSWWSSTRLGEKLNFARDRLMENFLWTVGVIFEPQYGYCRRMSTKVNTLITIIDDVYDVYGTMDELELFTDVVDRWDINAMDPLPEYMKLCFLALYNSTNEMAYDALKEHGLHIVSYLRKAWSDLCKSYLLEAKWYYSRYTPSLQEYISNSWISISGPVILVHAYFLVANPITKEALQSLERYHNIIRWSSMILRLSDDLGTSLDELKRGDVPKSIQCYMYETGASEEDARKHTSYLIGETWKKLNEDGAVESPFPETFIGIAMNLARMAQCMYQHGDGHGIEYGETEDRVLSLLVEPT",
    "response#2": "KNLVVVEFAKIDFNIVQAIHQEELKNVSSWWMETGLGKQLYFARDRIVENYFWTIGQIQEPQYGYVRQTMTKINALLTTIDDIYDIYGTLEELQLFTVAFENWDINRLDELPEYMRLCFLVIYNEVNSIACEILRTKNINVIPFLKKSWTDVSKAYLVEAKWYKSGHKPNLEEYMQNARISISSPTIFVHFYCVFSDQLSIQVLETLSQHQQNVVRCSSSVFRLANDLVTSPDELARGDVCKSIQCYMSETGASEDKARSHVRQMINDLWDEMNYEKMAHSSSILHHDFMETVINLARMSQCMYQYGDGHGSPEKAKIVDRVMSLLFNPN",
    "response#3": "KDLGLNNEVKFARYQPLKWYMWPMACFTDPRFSEQRIELTKPISLVYIIDDIFDVYGTLDQLTLFTDAIKRWELASTEQLPDFMKMCLRVLYEITNDFAEKIYKKHGFNPIETLKRSWVRLLNAFLEEAHWLNSGHLPRSAEYLNNGIVSTGVHVVLVHSFFLMDYSINNEIVAIVDNVPQIIHSVAKILRLSDDLEGAKSEDQNGLDGSYIDCYMNEHQDVSAEDAQRHVAHLISCEWL"
  },
  {
    "reference": "MQITIKDLQDLLSSVQRKKIQTLKLKKNKFELILNKPSKKVPQEVVSLKSSHIFKSIHSETINIPPKKTESINSKPSTNYATIVSPMVGTFYHSPAPGEKIFVQVGDIVKCNQTVCIIEAMKLMNEIEAEIEGIIIEILVKNGDIVDCGQALMKVET",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a specific function within the acetyl-CoA carboxylase complex, chloroplast.2. For general function, the protein need meet that This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.3. The protein design should take into account the physiological conditions of the fatty acid biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MTDVSRVLKEARDQGRLTTLDYANLIFDDFMELHGDRHFSDDGAIVGGLAYLAGQPVTVIGIQKGKNLQDNLARNFGQPNPEGYRKALRLMKQAEKFGRPVVTFINTAGAYPGVGAEERGQGEAIAKNLMEMSDLKVPIIAIIIGEGGSGGALALAVADQVWMLENTMYAVLSPEGFASILWKDGSRATEAAELMKITAGELYKMGIVDRIIPEHGYFSSEIVDIIKANLIEQITK",
    "response#2": "LPNNKVANAWDRVQISRMKDRPVGSDYIEALFTDFIEFHGDRYYADDKAIIGGVARFHGKPVTVIAQAKGTNTKENIERNFGMPSPDGYRKALRLMKQAEKFARPVICFVDTPGAFCGLEAEERGQGEAIARNIYEMSGLKVPVLSVIIGEGGSGGALAMATADEVWMLENSIYSILSPEGFASILWKDSTMAKEAAGVMKLTAGDLKRLGIVEQVFPEPAHYTVQNLKKVVTQLERRL",
    "response#3": "ELMKEREAKAKPFKVWDKVSAARQIKRLASVDYMDYIFDEFMELHGDRYFRDDPAIVGGIAYLDGQPVTVIGVHKGKDLEDCAKRNYGMPSPEGYRKALRLMKQAEKFNRPIITFVNTSGAYPGMEAEENGQGEAIARNLYEMSGIKVPILCLMIGEGGSGGALALSVGNEVWMMENATYSILSPEGFASILWKDGKRAKEAAEVMKITAHDLKELDVVDDIIPEFGGADEDALSSIGNYMKGNMKKFLEG"
  },
  {
    "reference": "MSSSKKILLCVTGSIAAYKSVFLLRLLLKNDYDVKVVMTQSATEFVKPLSFSAFLEDPVYTGLTDEAGDWTEHVKLALWADLILIAPITAQTISKLANGACDNLLSAIYLSAKCPVAIAPAMDRDMWKHAAVKRNLDLLKKDGVLEIPVGSGALASGLEGEGRMAEPELIHEWLENYFQKKTELKNKKVLITAGPTREPLDPVRFISNHSSGKMGYALAEAMANNGAEVFLVSGPVSIQTNYPNIKVISIETAQEMFDACHKLFSDTDIAIMSAAVSDYKPENKEESKIKKKSERLSVTFEKNPDILASLGKQKKKHQMLVGFALETDNEIENARQKLKSKNCDFIILNSLKDEGAGFQHDTNKVTLIDKNNNVNKFELKSKVELSKDLTEYLIQEYKSSFKTDDSK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein should be able to manipulate the activity of enzymes in the Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis pathway, either by activating or inhibiting their function.2. The designed protein should be able to compete with endogenous proteins or inhibitors for CTP binding.3. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.4. The protein design must be able to perform FMN binding, metal ion binding, phosphopantothenoylcysteine decarboxylase activity, phosphopantothenate--cysteine ligase activity in order to contribute to coenzyme A biosynthetic process.5. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..6. The protein contains novel DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "RVVLGVGGGIAAYKACEVLRGLTESGHDVRVVPTEAALRFVGAATFEALSGHPVHTGVFTEVPDVQHVRVGKDADLVLVVPATADLLARAAHGLADDLLTNTLLTARCPVAFFPAMHTEMWEHPATRDNVALLRSRGAVVTEPDSGRLTGADTGKGRLADPRELARVVELARDVLTGPLTGKHVVVSAGPTREPVDAVRVLTNRSSGRMGYAIAAAAWRRGARVTLISGPTEIAPPAGVGVVRAETAADMERAVRAALPEADALVMAAAVSDFRPAAPHAGKLKKEQAPDAIPLEASPDVLRVTRDARPSHLVAVGFALESSDGVANARAKLESKTLDLIVLNPAGEEGAGFETDTNRVTLLGRDGTAEEIPRAAKRAVADAVWERVVPLLR",
    "response#2": "HILLIIGGGIAAFKSLDVIRRLRERGASVTPVLTQAAEQFVTPLSVSSLAGRKVYRDLFDLTEEAEMGHIQLSRVADLVVIAPVTADLMAKMASGIANDLASTLLLATDTPVMIAPAMNVRMWEHSATQRNLQTLMEDGVTCVGPNDGDMACGEFGPGRMAEPLELVDAIAALSALGPSDLEGVRVVVSAGGTREPLDPVRYLGNRSSGKQGYALARVAAQRGAAVTLVAAHTLALPEPAGVDLVSVSTAEQLGKAVRTAAADADVVVMAAAVADFRPESLAEHKIKKADDGSAPVVTLARNPDILAGLVADRRPGQLIVGFAAETGDERGGVLDHARRKLARKGCDLLVVNAVGEGRAFEVEENTGWLLGADGTETPVPHGSKADLADAVWDHVVALLD",
    "response#3": "ELILGVGGGIAAYKSADLLRRLQDHGFLITVVPTRASLNFVGKATWEALSGREVHEDLWKNVSSVPHISLARSADAIVIAPATADLIAKIATGRADDLLTNIVSASASPIILVPAMHPEMWLNPATIENVKILRSRGLLVIDPDEGRLTGADSGPGRFPTSERIVHEVEDLDAVLSALEGPLTGKHVVVSAGPTREPVDAVRVLTNRSSGRMGYAIAAAAWRRGARVTLISGPTEIAPPAGVGVVRAETAADMERAVRAALPEADALVMAAAVSDFRPAAPHAGKLKKEQAPDAIPLEASPDVLRVTRDARPSHLVAVGFALESSDGVANARAKLESKTLDLIVLNPAGEEGAGFETDTNRVTLIGPDGVREIPSGPKEDLADVVWDLVASRLS"
  },
  {
    "reference": "MTARAAVILAAGQGTRMKSKTPKVLHKVGGRALLDHMIDTVAAIGCERIVVVVGTHSPEVRAQAAGRLGEAAVAVQETPLGTAHAVLAAKAALADFDGDVLITYADAPLLRPDDLQPLFDLRGAGADIALIAFEPVDPLRYGRVIRGAEGEVLRIVEAADATPEEATVRTCNAGMMVADRAALFGWLARVKDDNAKREFYLTDVVGLAAADDRLVLAHVAPEASVMGCDTPLQLSQAEAVFQQRRRDQFLAEGVAMLAPETAHFSWDTKIAAGAQIEQFVVFAPGVTVETGAVIRAFSHLEGAVVREGALIGPYARLRPGADIGEEAHIGNFVEVKKVAVGKGAKANHLSYLGDGTVGPGANIGAGTIFCNYDGFDKWDTHIGAGAFVGSNSSLVAPVAIGAGAYTGSGSVITQDVAADALALERGVQAEKPGWAARFRERKLAERAARGKK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must bind to Mg(2+) in order to perform its enzymatic function.2. A protein that is capable of catalyzing reactions within the UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway with high efficiency and specificity.3. The designed protein should have a binding site that is specific to the target UDP-N-acetyl-alpha-D-glucosamine ligand.4. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "SDLPKVMHPLGGAPLFAHSIFFGQALEPERVVLVTGHGGEQVAQAAVELGFEVDFCVQKEQLGTGHAVAQAAPALAGFSGDAVVLYGDTPFISAQTLQAMRDARNNHDVVVLGFDPAVDRAMAAAV",
    "response#2": "IILAAGKGTRMRSDKPKVLHAIAHKPLLKHVYDMSSQLHDNTITIVYGHGGDLLKNTLAELRAIWTEQREQLGTGHAVQQAIPQIADADKALILYADVPLLKLATVERLLALANSTS",
    "response#3": "SDLPKVMHPLGGAPLFAHSIFFGQALEPERVVLVTGHGGEQVAQAAVELGFEVDFCVQKEQLGTGHAVAQAAPALAGFSGDAVVLYGDTPFISAQTLQAMRDARNNHDVVVLGFTG"
  },
  {
    "reference": "MAMHDPHEKNGPDAVESRVQTDFPVERFGGNGSPGVRRIEVISSQFNLVHRIIFFFGVFLIAYVYGLDGTIRYTYQPLATAEYQSHSLLATVNVLRSVIAAAAQPTAAKIADVFGRVELILLSIVFYTVGTIVEASANHLYEFCAGAVLYQIGYTSVILLVEVLIADVTSTRSRLLFSYIPALPFLINTWISGNVTSAVLKVTSWQWGIGMFAIIYPVCTIPLLAVLFVGHLKARRANPQAYKFSLLDQGVGQFFIDLFWYLDVVGILLLIAILALILVPFTIAGGAVTQWKTAKVIAPLVIGVLCVPAFIVWERWCPHPMVPFKLLKDRAIWGALGIAVMLNTAWTLQGDYLYTVLIVSFDESVMSATRITSLYSFVSVITGTILGAIVIYVRRLKPFIVAGTLVFMAAFGILIRFRGGADGTNHAGIIGGQILLGFAGGLFPYPAQASVQVASKHKYLAVITGIYLATYNVGSALGNTISGAIWTQVLPGELENRLGNATLAAEVYANPFAFTQVNPVGTPDRDAVILAYKHAQRLLCITGICLTVPLIAFSLCIRNPRLTKEQTLKEAEEIDDK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Major facilitator transporter involved in ferrichrome (FC) and ferrioxamine B (FOB) uptake.2. The protein should be designed to localize specifically to plasma membrane in order to perform its role in transmembrane transport more effectively. The designed protein sequence is ",
    "response#1": "DGTSVEKVIDLNPDLIIVWTTQGADIKKLEKIAPTVAVKYDKLDNIEQLKEFAKMTGTEDKAEKWLAKWDKKVAAAKTKIKKAVGDKTISIMQTNGKDIYVFGKDFGRGGSIIYKDLGLQATKLTKEKAIDQGPGYTSISLEKLPDFAGDYIFAGPWQSGGDDGGVFESSIWKNLNAVKNGHVYKMDK",
    "response#2": "SVGANVVGSGSWSFDNPFLKSKLKNVKDVGDPISVEKVMELQPDLIVVMNEENVDKLKKIAPTVVIPYNTAKNVEDTVSMFGDIAGAKDQAKSFMADFNKKAEAAKKKIAGVIDKDATFGIYENTDKGEFWVFNDNGGRGGQAVYNALGLKAPEKIEQDVIKKGEMKQLSQEVIPEYAADYMFITDYNPKGESKTLDKLENSSIWKNLDAVKHNRVFIGS",
    "response#3": "RIVTDFYAGELLSVGANVVGSGSWSFDNPFLKSKLKNVKDVGDPISVEKVMELQPDLIVVMNEENVDKLKKIAPTVVIPYNTAKNVEDTVSMFGDIAGAKDQAKSFMADFNKKAEAAKKKIAGVIDKDATFGIYENTDKGEFWVFNDNGGRGGQAVYNALGLKAPEKIEQDVIKKGEMKQLSQEVIPEYAADYMFITDYNPKGESKTLDKLENSSIWKNLDAVKHNRVFINDFDSFYPYDPISVSKQVDIITDMLIKRP"
  },
  {
    "reference": "MIEQFQQVLASVKSVDMSLTKEVQAHLDDLTKPRGSLGRLEEIAMKYVLATGSLRPELRKKKVFCFAADHGVAVEGVSAFPAEVTPQMVYNMLAGGAAINVLSRHAGADLDVVDMGVHHDFVEHPSLRICKVRHGSSNMAEGPAMTMDETLQAIMTGVSLAHEARKEGYDLLATGEMGIANTTPATALYASMLDLPVEMITGRGTGIDDAVLQHKISVIKRALDVNSASLSTPLEQLAALGGFEIAGICGLILGAASVGMPVVVDGFISSAGAVAALKLSCRVSDYLFFSHLSNEQGHKAIMNRLGARPILDLDLRLGEGTGAALAMQVVEASVKLYNEMATFSSASVSGKSV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should interact with enzymes and substrates within the Nucleoside biosynthesis pathway to enhance or regulate its activity.2. We are looking for a protein with broad substrate specificity for 5,6-dimethylbenzimidazole + nicotinate beta-D-ribonucleotide =   alpha-ribazole 5'-phosphate + H(+) + nicotinate. The designed protein sequence is ",
    "response#1": "FKFADMSTRRRFSFQAQRTMLEYLSKELPQCLTGTSNYHLARELDLTPIGTVAHEWFMGHQALVNVRDSQKIALQRWQKMFNGALGIALTDTIGIDAFLKDFDEELSHAYVGVRHDSGCPFTWGEKMIAHYESLGIDPMTKTLVFTDGLNFEQALDICEHFQGRVQVSFGIGTSLANDMGNYVNDQGEAYQPLSIVIKMVTCNGSPVAKISDEPEKAMCEDIFFLMNLKRRFEQA",
    "response#2": "FKFADMSTRRRFSFQAQRTMLEYLSKELPQCLTGTSNYHLARELDLTPIGTVAHEWFMGHQALVNVRDSQKIALQRWQKMFNGALGIALTDTIGIDAFLKDFDEELSHAYVGVRHDSGCPFTWGEKMIAHYESLGIDPMTKTLVFTDGLNFEQALDICEHFQGRVQVSFGIGTSLANDMGNYVNDQGEAYQPLSIVIKMVTCNGSPVAKISDEPEKAMCEDIFFLMNLKRRFEQR",
    "response#3": "FKFADMSTRRRFSFQAQRTMLEYLSKELPQCLTGTSNYHLARELDLTPIGTVAHEWFMGHQALVNVRDSQKIALQRWQKMFNGALGIALTDTIGIDAFLKDFDEELSHAYVGVRHDSGCPFTWGEKMIAHYESLGIDPMTKTLVFTDGLNFEQALDICEHFQGRVQVSFGIGTSLANDMGNYVNDQGEAYQPLSIVIKMVTCNGSPVAKISDEPEKAMCEDIFFLMNLKRRFEQY"
  },
  {
    "reference": "MAILRLEDGTLHTDIQDIAHELAPLNIQLNYWSVGNHPELQRLLAQDSLNDAEKDQVLKALDSYFEQLQQTAGYQSRDLIVLHPGVPNLNELLAKFEPIHTHADDEVRYIIDGEAVFGFVRPDGSQVELTVQPEEYINVPALTEHWFYLTPARRIKAIRYFINTEGWIPQYTGKNSKFKIQESFR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have iron ion binding to facilitate L-methionine salvage from S-adenosylmethionine.2. The protein must be engineered to have a cooperative binding mechanism with Ni(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "SSQVGCALDCKFCSTAQQGFNRNLRVSEIIGQVWRAAREVGLEKETGRRPITNIVMMGMGEPLLNMKNLIPSLEIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIDKIDVALAISLHAPNDALRSQIMPINDRWNIDAFLDVVRRYIATSNANRGKVTVEYVLLDHVNDGTEHR",
    "response#2": "DGDRATLCVSSQVGCALDCKFCSTAQQGFNRNLRVSEIIGQVWRAAREVGLEKETGRRPITNIVMMGMGEPLLNMKNLIPSLEIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIDKIDVALAISLHAPNDALRSQIMPINDRWNIDAFLDVVRRYIATSNANRGKVTVEYVLLDHVNDGTEHAEELAQLMRGTPCKINLIPFNPYPGSPYNKPSNSRIDRFQKTLMKHEHTVTIRKTRGDR",
    "response#3": "SSQVGCALDCKFCSTAQQGFNRNLRVSEIIGQVWRAAREVGLEKETGRRPITNIVMMGMGEPLLNMKNLIPSLEIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIDKIDVALAISLHAPNDALRSQIMPINDRWNIDAFLDVVRRYIATSNANRGKVTVEYVLLDHVNDGTEHR"
  },
  {
    "reference": "MDSQVTSSAKTPNPVVFFDITLGGESLGRIKMELFTSITPRTAENFRQFCTGESKSPQGRPQGYKNSKFHRVIKDFMIQGGDFVNGDGTGSRTIYGTPRFQDENFILKHDQPGLLSMANSGPNTNGCQFFITTTATPFLNNKHVVFGQVVEGMDVVRMIENTRTTRDKPNQDVTIIQCGEM",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that PPIases accelerate the folding of proteins; It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides .2. The protein can be expressed in nucleus and perform peptidyl-prolyl cis-trans isomerase activity without triggering undesirable cellular responses.3. The presence of PPIase cyclophilin-type domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "IVLDGYNAPVTAGNFLDLVERHFYDGMEIQRRDGFVVQTGDPEGPAEGFIDPSTEKPRTIPLEIMVEGEKVPVYGSTLEELGLYKAQTKLPFNAFGTMAMAREEFENNSGSSQIFWLLKESELTPSNANILDGRYAVFGYVTDNQDYLADLKVGDVIESVQAVSGVDNLVNPTYKIAQ",
    "response#2": "ETSMGPFTVEMYYKHSPRTCRNFLELSRRGYYDNVLFHRIVKDFIVQGGDPTGTGRGGESIYGSKFEDEINKELKHTGAGILSMANAGPNTNGSQFFITLAPQPSLDGKHTIFGRVCRGMEVIKRLGSVQTDNTDRPIHEVKILRTKK",
    "response#3": "VTLETSMGPFTVEMYYKHSPRTCRNFLELSRRGYYDNVLFHRIVKDFIVQGGDPTGTGRGGESIYGSKFEDEINKELKHTGAGILSMANAGPNTNGSQFFITLAPQPSLDGKHTIFGRVCRGMEVIKRLGSVQTDNTDRPIHEVKILRTKK"
  },
  {
    "reference": "MLDPKYLRSEIDEAAARLATRGYVLDVAAVNALEEKRKDLQSRTQELQAERNARSKSIGEAARRGEDVAPLKAEVTKINDELETSKIELDALLTEIKSISDAIPNLPSETTPVGRDENDNVEVRRWGTPRQFTFPVRDHIDLGEAAKGVDFKNGVKLSGARFVVMKGQIARLHRALAQFMLDLHTLQHGYTECYVPYLVNPDSLYGTGQLPKFSQDLFNTGIEGEGEDEGKMRKFSLIPTSEVPLTNMARDEIFDEQELPIKMTAHSPCFRSEAGSYGRDTRGLIRMHQFDKVEMVQLVHPEKSWEALEEMAGHAEKVLQLLELPYRVMALATGDMGFCAAKTYDLEVWLPAQNTYREISSVSNCTDFQARRMQARVRIDGKPQLLHTLNGSGLAVGRTLVAVIENYQQEDGRIAIPAALQSYMGGLTHIG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design must be focused on enhancing the efficiency of seryl-tRNA aminoacylation. The designed protein sequence is ",
    "response#1": "DNPVAKTWGSKPELDFEPKPHWEIGEALGILDFERAAKIAGARFALYWKMGAALERALIAFMLDIHTKRHGYTEVLPPFIVNSTSLLGTGQLPKFKEDLFKLEDRDFYLVPTAEVPVTNIHMNETLEESELPKLYTAFTPCFRSEAGSYGKDTRGLIRQHQFNKVELVKLVKPESSYEELEKLLLDAERILQELGLHYRVVTLCTGDMGFSSAKTYDIEVWLPGQNTYREISSCSNFEDFQARRANIRFRRKGQSKTEFVHTLNGSGLAVGRTLVAILENGQQADGSVSIPPALRPYLG",
    "response#2": "DNPVAKTWGSKPELDFEPKPHWEIGEALGILDFERAAKIAGARFALYWKMGAALERALIAFMLDIHTKRHGYTEVLPPFIVNSTSLLGTGQLPKFKEDLFKLEDRDFYLVPTAEVPVTNIHMNETLEESELPKLYTAFTPCFRSEAGSYGKDTRGLIRQHQFNKVELVKLVKPESSYEELEKLLLDAERILQELGLHYRVVTLCTGDMGFSSAKTYDIEVWLPGQNTYREISSCSNFEDFQARRANIRFRRKGQSKTEFVHTLNGSGLAVGRTLVAILENGQQADGSVSIPPALRPYLG",
    "response#3": "DNPVAKTWGSKPELDFEPKPHWEIGEALGILDFERAAKIAGARFALYWKMGAALERALIAFMLDIHTKRHGYTEVLPPFIVNSTSLLGTGQLPKFKEDLFKLEDRDFYLVPTAEVPVTNIHMNETLEESELPKLYTAFTPCFRSEAGSYGKDTRGLIRQHQFNKVELVKLVKPESSYEELEKLLLDAERILQELGLHYRVVTLCTGDMGFSSAKTYDIEVWLPGQNTYREISSCSNFEDFQARRANIRFRRKGQSKTEFVHTLNGSGLAVGRTLVAILENGQQADGSVSIPPALRPYLG"
  },
  {
    "reference": "MTVNGKDIDSPNAQYVAAGIDMSDLFPAPFPTNVKTVHLETLSLAKLLQRDEDELRRIYENCKDPGFFQLDLTDDEQGVQLLQDAVDCARLMKQLLPNMSVEEKRMYKQHSRVGVYSKGYQVYDVLPNGQPKYNETVNFPMTEMLGYGDSTVDLPDWLSPHRELFQRTMRSGNKIANIVLAALEVGLQVPRGALTDAHRIQDPSDDFLRLLRYPGLQPGQPRDDLCFPAHKDFTSLGILFTWLGGLQLLASASAPGVTSGTMTGPLDIAEDAWRWVQPVPGTAIVNVGNALEILTNKALTSGLHRVVRAPGEQLPFDRYSVLVGTRPANSFPMKPLQSPQISPVLDPAAAEIATMTSGQWGTHNIGSFNNWVKARTERQEVLIIP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that 2-oxoglutarate-dependent dioxygenase; part of the gene cluster that mediates the biosynthesis of fumigermin that inhibits germination of spores of the inducing S.rapamycinicus, and thus helps the fungus to defend resources in the shared habitat against a bacterial competitor; It is remarkable that the five cluster genes including fgnA are conserved in distantly related fungi, supporting the assumption of a fumigermin cluster; it is thus possible that originally all five genes were functional, but that the genes encoding tailoring enzymes became inactive from mutations, similar to the case of the fgnA gene in strains A1163 and Af293 (Probable).2. The protein must be able to metal ion binding. The designed protein sequence is ",
    "response#1": "IAICGLGLRLPGGIRDGDSFWDLLVNGRDARMPIPASRYNISGFDGSLDGRDPIKTTHGYFLDEDLSSLDASFFSMTKTELEKCDPQQRQLLEVTRECLEDAGETDYRGRNVGCYIGNFGHDWMEISLREPQHSRSYNVLGYSDMILANRVSYEYDLRGPSVVIKTACSASLVALHEACRALQARDIPSAIVGGTSLILAPTLTSNFFGEGILSPEASCKTFDESADGFARAEGVTAIYVKRLDDALRDGNPIRAVIRGTGTNSDGKSMGIMSPSAEAHEALMRQVYDQAGLSPRETAFVEEKRRLSNASKCHGTGTATGDPIEATAVGNVFSERGVYIGSVKPNVGHSEGCSGITSLIKAVLALEHKTIPPNIKFRNPNPKIPFVEKKLVVPIQPTPFPLDKAERISVNSFSIGGSNAHIDAYKHSLLGERTVDSPDFSPIWRNMLSLEDVTWIADHKIRRDVVFPLAGYIAMAGEAIRQTTGVDVGYRLRDVEARKALVLTDFKATEVVTVLHAHTGVPAEGPSWYDFSIASCHGGSWLIHCTGQVSPLAETLSLSWEPESASQRSLPRELTPMRFYEAMARVGVVFGPEFQRLVDITSSATDPLAEARVMSPAPRENKRPFALHPTAIDACIQLLIVAAARGLCRNLDQLEVPVVVGNVEVVSRGSANLRALAQDFTQGVECVTENSKLALRMSGLQLAPLAAEVAGG",
    "response#2": "IAICGLGLRLPGGIRDGDSFWDLLVNGRDARMPIPASRYNISGFDGSLDGRDPIKTTHGYFLDEDLSSLDASFFSMTKTELEKCDPQQRQLLEVTRECLEDAGETDYRGRNVGCYIGNFGHDWMEISLREPQHSRSYNVLGYSDMILANRVSYEYDLRGPSVVIKTACSASLVALHEACRALQARDIPSAIVGGTSLILAPTLTSNFFGEGILSPEASCKTFDESADGFARAEGVTAIYVKRLDDALRDGNPIRAVIRGTGTNSDGKSMGIMSPSAEAHEALMRQVYDQAGLSPRETAFVEEKRRLSNASKCHGTGTATGDPIEATAVGNVFSERGVYIGSVKPNVGHSEGCSGITSLIKAVLALEHKTIPPNIKFRNPNPKIPFVEKKLVVPIQPTPFPLDKAERISVNSFSIGGSNAHIDAYKE",
    "response#3": "LLRSTVFISICVGFGLAFLGLFNPFFYISSYAAGHGASAQTSSYMISIMNAATLFGRVIPGIVADRVGHYNVMIFVLLASGITSFCWTEVRSLTGLVIWSIAYGFSSGAILSLQGACAGKIATPQNQGKAIGFLQGSLAVTVLVGSPIGGQLLGHYGYLSLSMFTGATLVMGAVVMGYARLCLNRSPMEAHQFRFLLAPV"
  },
  {
    "reference": "MKIDIFGMFVDEIAQVIHPYGLPKYRALQIAKWMYQCGVYDFDNMTNLSKDHRALLMDNFVIHTVVTQAVQQSSDGKTSKYLVSFHDGSAVETVLMRQPYGNSVCVSTQVGCAMGCVFCASTLNGVIRNLSGGEILAQVLYINSLLQQEQAAVNNIVIMGSGEPLANYDNVLKFIRLCHEEYSIHLSYRSITLSTSGLVPEMNQLAREGLPVTLSISLHAPTNEIRSQLMPINNRYSLSEVMDAGDYYAEQTGRRVTYEYVLISDVNDKIEHAGQLAALMKGRLANVNLIPVNPVMERGLLRPDNKRIQQFEKNLLDKRINVTVRREMGTDIRAACGQLRNNVVNNSPKAR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have 4 iron, 4 sulfur cluster binding, metal ion binding, tRNA (adenine-C2-)-methyltransferase activity, rRNA binding, tRNA binding within cytoplasm to regulate rRNA base methylation.2. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs..3. The protein should have a high affinity for S-adenosyl-L-methionine.4. The S-methylcysteine intermediate, Proton acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.5. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity.6. The presence of [4Fe-4S] cluster is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MPPQHLADLDEAGRAAAVAGLGLPAFRAKQLANQYYGRLIADPHLMTDLPAAVREQVAEALFPPLLKMVRHIECDAGETRKTLWRAGDAATFESVLMRYPQRATVCISSQAGCGMACPFCATGQGGLTRNMSTAEILEQVRAASAVMRDEHAGRLSNVVFMGMGEPLANYNRVLGAVKRIIAPPPHGFGISARSVTVSTVGLAPAIRKLADERLGVTLALSLHAPDDELRDTLVPVNNRWNVGEALDAARYYADVTGRRVSIEYALIRDVNDQPWRGDLLGKKLHAALGPLVHVNVIPLNPTPGSKWDASPKPAEREFVKKVRERGVSCTVRDTRGREIAAACGQLAAD",
    "response#2": "MPPQHLADLDEAGRAAAVAGLGLPAFRAKQLANQYYGRLIADPHLMTDLPAAVREQVAEALFPPLLKMVRHIECDAGETRKTLWRAGDAATFESVLMRYPQRATVCISSQAGCGMACPFCATGQGGLTRNMSTAEILEQVRAASAVMRDEHAGRLSNVVFMGMGEPLANYNRVLGAVKRIIAPPPHGFGISARSVTVSTVGLAPAIRKLADERLGVTLALSLHAPDDELRDTLVPVNNRWNVGEALDAARYYADVTGRRVSIEYALIRDVNDQPWRGDLLGKKLHAALGPLVHVNVIPLNPTPGSKWDASPKPAEREFVKKVRERGVSCTVRDTRGREIAAACGQLAAGS",
    "response#3": "MPPQHLADLDEAGRAAAVAGLGLPAFRAKQLANQYYGRLIADPHLMTDLPAAVREQVAEALFPPLLKMVRHIECDAGETRKTLWRAGDAATFESVLMRYPQRATVCISSQAGCGMACPFCATGQGGLTRNMSTAEILEQVRAASAVMRDEHAGRLSNVVFMGMGEPLANYNRVLGAVKRIIAPPPHGFGISARSVTVSTVGLAPAIRKLADERLGVTLALSLHAPDDELRDTLVPVNNRWNVGEALDAARYYADVTGRRVSIEYALIRDVNDQPWRGDLLGKKLHAALGPLVHVNVIPLNPTPGSKWDASPKPAEREFVKKVRERGVSCTVRDTRGREIAAACGQLAAL"
  },
  {
    "reference": "MIINVFYKTKVPSHFRKTSLFKAGVSAALGKFASKKGEVNLIFVDGKEIHKINKEFLNHDYKTDVISFNYPFPQKGGEGLPFGDIFVCYDVAKENASLYGQGVLKEMLTYAVHGALHLAGMDDATPKERSAMDDETGRIILKI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "MMAWMNAESVAKTLETGRVTYWSRSRQSFWIKGESSGHVQELVELRVDCDRDALLAMVRQTGPACHTNRRSCFG",
    "response#2": "MMAWMNAESVAKTLETGRVTYWSRSRQSFWIKGESSGHVQELVELRVDCDRDALLAMVRQTGPACHTNRRSCFN",
    "response#3": "MMAWMNAESVAKTLETGRVTYWSRSRQSFWIKGESSGHVQELVELRVDCDRDALLAMVRQTGPACHTNRRSCFL"
  },
  {
    "reference": "MLKVNEYFDGKVKSIGCSNSEGELTVGVMAVGEFVFSTSKKEFMTVVSGELEVLLPGESEKRTIVKGEQFIVPANEKFDVKVAQETIYTCRYE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be able to perform pyrimidine-nucleoside phosphorylase activity under specific conditions.2. The protein have enhanced phosphate + xanthosine = alpha-D-ribose 1-phosphate +   xanthine.3. For general function, the protein need meet that Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates; Also catalyzes the reverse reactions. The designed protein sequence is ",
    "response#1": "MLKVNF",
    "response#2": "MPN",
    "response#3": "LXGXYXV"
  },
  {
    "reference": "MVSKEKINRINELARIAKERVLTKLEEEEQQILRKEYIEAFRKSFRKQLDNIELVD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECL",
    "response#2": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECR",
    "response#3": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECA"
  },
  {
    "reference": "MTDQQLDYQVTVEELSALMKRTAGVHVDPVTLRQQADDGFDTFGLDSLGLLGIVAELEKRYGLGLPEQAERCKTPADFLALVNGALKTGV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should contain one or more Carrier domains that are essential for its function. The designed protein sequence is ",
    "response#1": "VRRRVYTRRHNRDQHDGGDTLCALIYCNQNHTARVLFELLAEMGKRDADLKFLRCQYTTDRVADPTTEPKEAELEHRRQEEVLKRFRMHDCNVLIGTSVLEEGIDVPKCNLVVRWDPPTTYRSYVQCKGRARAAPAYHVILVAPRYTSPAIGVVQLTDKSHRFICGAGGGAADSTEVDSDSDDSAMPNSLGSDPYTFGTARGTVKILNPEVFSSVPPMACEIL",
    "response#2": "SDMQSILDLHNKLRGQVYPPASNMEYMTWDTELERSAESWAETCLWEHGPASLLPSIGQNLGAHWGRYRPPTFHVQAWYDEVRDFTYPHPHECNPYCPYKCSGPVCTHYTQVVWATSSRIGCAINLCHNMNIWGQIWPKAVYLVCNYSPKGNWWV",
    "response#3": "VRRRVYTRRHNRDQHDGGDTLCALIYCNQNHTARVLFELLAEMGKRDADLKFLRCQYTTDRVADPTTEPKEAELEHRRQEEVLKRFRMHDCNVLIGTSVLEEGIDVPKCNLVVRWDPPTTYRSYVQCKGRARAAPAYHVILVAPRYTSPAIGVVQLTDKSHRFICGAGGGAADSTEVDSDSDDSAMPNSLGSDPYTFGTARGTVKILNPEVFSSVPPMACEII"
  },
  {
    "reference": "MYLSPQEKDKLLIYLAAQLARDRRQRGLKLNYPEAVAYLSAAVLEGAREGKTVAQLMSEGLTWLTREDVMPGIPEMIPEIQVEATFPDGTKLVTLHHPIR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein should be able to manipulate the activity of enzymes in the Nitrogen metabolism, urea degradation pathway, either by activating or inhibiting their function.2. The protein have enhanced 2 H(+) + H2O + urea = CO2 + 2 NH4(+).3. A protein that localizes to cytoplasm and can perform urease activity with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "GGIDSHIHFICPQQIEEALASGITTMLGGGTGPATGTFATTATPGPWHIERMLQAADAFPMNLGFLGKGNASLPDALHEQIEAGVMGLKLHEDWGTTPSAISNCLDVADATDTQVAIHSDTLNESGFVENTIAAVGGRSICAFHTEGAGGGHAPDILRVVGEENFLPSSTNPTMPYTVNTLDEHVDMLMVCHHLDAGIAEDLAFAESRIRKETIAAEDVLHDLGAISMFSSDSQAMGRVGEVVLRCWQTAHKMKLQRGKLPEDSERNDNFRVKRYVAKYTINPAISHGIAHEVGSIEVGKWADLVIWKPAFFGVKPFTLIKGGTIAMAAMGDPNASIPTPQPVHYRPMFGSYGGSLARSSLTFVSQAGLAAGIKERYGLAKHLSAVKNIRNVRKKDLVHNGYTPKMEIDAQTYAVRADGHLLTCDPAVQLPLTQRYFLSAADY",
    "response#2": "GGIDSHIHFICPQQIEEALASGITTMLGGGTGPATGTFATTATPGPWHIERMLQAADAFPMNLGFLGKGNASLPDALHEQIEAGVMGLKLHEDWGTTPSAISNCLDVADATDTQVAIHSDTLNESGFVENTIAAVGGRSICAFHTEGAGGGHAPDILRVVGEENFLPSSTNPTMPYTVNTLDEHVDMLMVCHHLDAGIAEDLAFAESRIRKETIAAEDVLHDLGAISMFSSDSQAMGRVGEVVLRCWQTAHKMKLQRGKLPEDSERNDNFRVKRYVAKYTINPAISHGIAHEVGSIEVGKWADLVIWKPAFFGVKPFTLIKGGTIAMAAMGDPNASIPTPQPVHYRPMFGSYGGSLARSSLTFVSQAGLAAGIKERYGLAKHLSAVKNIRNVRKKDLVHNGYTPKMEIDAQTYAVRADGHLLTCDPAVQLPLTQRYFLQR",
    "response#3": "GGIDSHIHFICPQQIEEALASGITTMLGGGTGPATGTFATTATPGPWHIERMLQAADAFPMNLGFLGKGNASLPDALHEQIEAGVMGLKLHEDWGTTPSAISNCLDVADATDTQVAIHSDTLNESGFVENTIAAVGGRSICAFHTEGAGGGHAPDILRVVGEENFLPSSTNPTMPYTVNTLDEHVDMLMVCHHLDAGIAEDLAFAESRIRKETIAAEDVLHDLGAISMFSSDSQAMGRVGEVVLRCWQTAHKMKLQRGKLPEDSERNDNFRVKRYVAKYTINPAISHGIAHEVGSIEVGKWADLVIWKPAFFGVKPFTLIKGGTIAMAAMGDPNASIPTPQPVHYRPMFGSYGGSLARSSLTFVSQAGLAAGIKERYGLAKHLSAVKNIRNVRKKDLVHNGYTPKMEIDAQTYAVRADGHLLTCDPAVQLPLTQRYFLSAADYC"
  },
  {
    "reference": "MKFLDLCKVYIRSGAGGGGCVSFRREKYIEYGGPDGGDGGTGGSVWAEAVDGLNTLIDFRYQQHFFAKNGQPGMGRQRTGKDGDDIVLRVPVGTEILDEDQETVICDLTEVGQRVQLARGGNGGFGNLHFKSSTNQAPRRSNPGQDGVERTLWLRLKLIADVGLLGLPNAGKSTFLAATSNARPKIADYPFTTLHPNLGVVGVDNTEFVVADIPGLIEGASEGRGLGDLFLGHIERCAVLLHLFDGTSETLIEDYHTIIGELEAYGVGLADKPRITVLNKIDALDEERRAMALKQLNNVCGGGVMAMSGVAGDGVTDVLRKLRGEISDEPLRHKPVEEKEPWRP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "HWLMKSEPESRLEKGVDVKFSIEDLKAQPKQTTCWDGVRNYQARNFLRAMKLGEEAFFYHSNCKEPGIAGLMKIVKEAYPDHTQFEKNNPHYDPSSKEDNPKWSMVDVQFVRMMKRFIPLAELKSYHQAHKATGGPLKNMVLFTRQRLSIQPLTQEEFDFVLK",
    "response#2": "HWLMKSEPESRLEKGVDVKFSIEDLKAQPKQTTCWDGVRNYQARNFLRAMKLGEEAFFYHSNCKEPGIAGLMKIVKEAYPDHTQFEKNNPHYDPSSKEDNPKWSMVDVQFVRMMKRFIPLAELKSYHQAHKATGGPLKNMVLFTRQRLSIQPLTQEEFDFVLR",
    "response#3": "HWLMKSEPESRLEKGVDVKFSIEDLKAQPKQTTCWDGVRNYQARNFLRAMKLGEEAFFYHSNCKEPGIAGLMKIVKEAYPDHTQFEKNNPHYDPSSKEDNPKWSMVDVQFVRMMKRFIPLAELKSYHQAHKATGGPLKNMVLFTRQRLSIQPLTQEEFDFVLK"
  },
  {
    "reference": "MELNISESRSRSIRCIVKLGGAAITCKNELEKIHDENLEVVACQLRQAMLEGSAPSKVIGMDWSKRPGSSEISCDVDDIGDQKSSEFSKFVVVHGAGSFGHFQASRSGVHKGGLEKPIVKAGFVATRISVTNLNLEIVRALAREGIPTIGMSPFSCGWSTSKRDVASADLATVAKTIDSGFVPVLHGDAVLDNILGCTILSGDVIIRHLADHLKPEYVVFLTDVLGVYDRPPSPSEPDAVLLKEIAVGEDGSWKVVNPLLEHTDKKVDYSVAAHDTTGGMETKISEAAMIAKLGVDVYIVKAATTHSQRALNGDLRDSVPEDWLGTIIRFSK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must be able to kinase activity, ATP binding.2. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.3. For general function, the protein need meet that Regulates the production of farnesyl diphosphate- derived terpenoids in the cytosol, and geranyl diphosphate-derived compounds in plastids.; Catalyzes the formation of isopentenyl diphosphate (IPP), the universal five-carbon isoprenoid building block of all natural isoprenoids The designed protein sequence is ",
    "response#1": "MDFRIGQGYDVHALVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDAIFGAAAMGDIGRHFSDTDAKFAGADSRVLLRECFARVKAAGFSIANVDSSVVAQAPKLAPHIEGMRANIAADLGLPVERVNVKAKTNEKLGYLGRGEGIEAQAAVLLIL",
    "response#2": "MDFRIGQGYDVHALVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDAIFGAAAMGDIGRHFSDTDAKFAGADSRVLLRECFARVKAAGFSIANVDSSVVAQAPKLAPHIEGMRANIAADLGLPVERVNVKAKTNEKLGYLGRGEGIEAQAAVLLIY",
    "response#3": "MDFRIGQGYDVHALVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDAIFGAAAMGDIGRHFSDTDAKFAGADSRVLLRECFARVKAAGFSIANVDSSVVAQAPKLAPHIEGMRANIAADLGLPVERVNVKAKTNEKLGYLGRGEGIEAQAAVLLIL"
  },
  {
    "reference": "MTAAQVLTAAQMRAAEQALFDAGTSVSELMEIAAGGAAEWIRRVAAGRAVTVLCGPGNNGGDGYVIARRLRDAGNAVAVIAPLDPATDAAKEARRRWDGPVSTAGGAGADVFVDCLFGSGLARPLVAEHALLIRDLAARHRYKVAVDVPSGIASDSGAVLNERLPDYDLTLALGAWKFAHWSLPGRSLMGQMRLIPIGIAPLEGAAQLVERPRLSAPAADSHKYRRGLLGIVTGAMPGASLLAAAAAQRSGAGYVKLLAPHVDPRTPPDVVTDTAPLAEALADPRIAAVLVGPGLGRDEAARERLEAALAQARALVLDADALMLLTPAMLAREVPRLVTPHDGELEALCRSFGVIAETRRDRALALARVSGMVVLAKGPDSIVVAPDGRIALGQPAPSWLSVAGTGDVLAGIAASRMANGADPFNAACEALWLHGEAARRAGAAFTPTQLAERVSEALAACL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..2. The protein design must be able to perform NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, metal ion binding, ATP binding in order to contribute to nicotinamide nucleotide metabolic process.3. The designed protein should have a high specificity for AMP, K(+), (6S)-NADPHX binding, with minimal off-target effects.4. The protein contains novel YjeF N-terminal domains that confer a unique function or activity.5. The desired protein should be able to perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate The designed protein sequence is ",
    "response#1": "MEKSDVKTLPARKPWGNKGTFGKVLLVAGSKGMCGAACLSASAALHGGAGMVKIQTVEENRIPLQSLLPEAMLTSEFDEEANRKNLNWCDVLVIGPGLGTEGSGKERMLWFLENGTKAGKPVILDADGLNILAMHPQWMKFIGKQTILTPHMGEMSRLCGLDVSKLKEDPVARAYQYAEKSGGVLVLKDACTVVTDRNEKLYLNLSGNSGMATAGSGDVLSGIIAAVLCMYLSCEEEQELSYKAALAVYIHGLCGDIAREKKGSHGMTAKDMIEALPEVLKLAEVGCTGPITDPTPGARSDKYSGGVVALAAGSDTYPGAGVLCATAAVRATPSMVRFIGDNTITSLLPELVCHPDVPSSGRAQAWVVGPGRGTDSAAATELRKVLAQGLPTVIDADALTLLATNTSLRQLVAAHPLTILTPHEGEFTRLYEAAFSAAPSAAWGDALHRLAEELNSIILLKGRLTRVTAPGQPIYSCNAGHSFAATPGSGDVLSGILGAVIAQLSADTPVEVITEVLHAGAIHAHAAAIAAETPDGLAPCSASQIAAAIPQAIARLLSPAEMAEADRLAIAAGPLDGYGLMQRAGEAVAAMVLARYPAAKKVHVLCGPGNNGGDGYVVARLLAEAGVDVELWAEGKQKPGSDAALASEACNLPVNHIGHCYPEAGDVVVDALYGAGLSKDISQHALAVSGVSSAERVPVISIDLPSGVSGETGQMRGHFDAAVTVTFARKKPGHLLMPGRRVCGEIIVADIGIDGADILGWWPELDAASEKYTRGVVGVATGSATYPGAAVLSVAGALAGPTGLVRYAGGAKAEVLHHHPSVIATERVSDAGRVQAWVCGSGLGSGEPAATALRTVLAAPVPVVLDADGLTLLVDGSAADRLRDRDAPVVVTPHDREFARLAGESPGSDRVGAAQRLAAWMNAVVLLKGDRTIVATPAGRAYANPTGTPALATGGTGDVLAGLLGSLLAAGLAPEHAAVAAAYLHGLAGRAAARRGPVTAPDVATALRDVVPADQ",
    "response#2": "MKRLDQAAIKEYGVPGLVLMENAGRQVVEVIGDVLGIVRGKVVTVFIGKGNNGGDGLVIARHLLNMGAEVKVLALAGVEEITGDAAVNLEIWRKMGQKIYSLHHGDGINIVRLILMNSELIVDAIYGTGFRGKMEEKVGRIVEVLNGSGKPIIAVDIPSGLEANTGRVNEPCIQAAHTVTFALPKLGLILEPGADYAGELHVADISIPSVLLGDDLQVYAACAAHAPSHKGEAGKVLVIGGCRAYAGAPCLSALGAFRAGAGLVTVAGPGPVPAALRAACPAAISAVLPDRSDGKGWLASDADVLIPAMLRADAVVLGPGLGRGEEEEQLVELLVSAPERPPMVIDADALFAVGSRPETLRHLQAGDILTPHPGEAAALLGSTSREVQEDRTGALLRLTGLCPSACVLKGEGTLIGRRDEASVISPWRVPQLAVAGSGDVLSGIAAALLAQHRDAVEAAAVAVWTHALAGMRIAPSFPMRGNTPQDIADAVPHALAAACRDAENAAFARGIPAEALMNQAGAGIARAVQQFFSRPGYCLVFSGKGNNGGDALVAAAHLKRAGWHIDVHLSFPESECSELLRKKVTEFRDTPAVAAAASAANADGTPAPIIILDGLLGLGSRPPLRDPIIAACREINRLRREQNAYVFAVDQPTGLDGDSGKADSDCVVADFTVTIAAAKRGLVADDATNFVGRLETNKGSFGSLAVVGGDTGMCGAPILAARAALFSGAGKVHVALLGAGAPPYDPPHPELMLHDIGELPLDRMDALAIGCGMGQRERASHVMRDVLQLDVPKLFDADALNLIATDPALATAVTARGVQGDPCILTPHPLEAARLAGTDAPGVQRDRLTAARALAARFASVVVLKGSGTIIAAPDGRLAVNPTGNAALATGGTGDVLGGIIGALLAQRLPRYEAALAGVYLHGLAADTLSAQGHGLAGLT",
    "response#3": "MAAGSLRRLAPRPRTAHKGQLGHLLVVGGDRGLGGAALLAAESALRSGAGLVSLATRPEHVMAALARRPEVMCAGVSSANQLLALADRADVLVAGPGLGQDAWGRGLLSVVAGLESVQVWDADALNMLAGGLVRLPAGCVITPHPGEAARLLGISTAEVQADRPRAVLALAQKFDAVVLLKGAGSLVAAPDGRLALCDRGHPVMAGAGLGDVLAGLIGALLAQGMDAYSATCLAVWLHACAGEGLARGGRGLAASDLIPTIRQLLEEFAEPSDIGAMLPARSVDAHKRSTGSVLVLAGSDGMSGAALLTSRGAARMGAGYVTLVSTSYVDHAKKSLIPEAVSRIVADHSELGPEVLAEFDDDFDRADAVALGPGLGTGPRQQALVETALEAIDVPLVLDADGLNVLAEDAAALRKRTSPTVLTPHPGEMATLLKLSSKDVQADRVGVARRAASDLGCVVVLKGAGTIVAGPEGPAVVNPTGGPELATAGTGDVLTGVVGALLAEGLDPVSAAIAGVFVHGLAGGVAGVSAGGRGTVAWDVAEALPAAVGELYEQH"
  },
  {
    "reference": "MKFGKEFSSQMVPEWHEAYMDYDYLKSQLKEIIKFKRKTNPHGPHHHHHHLLHRKMTLHRAFSGLISTSPKKKKHHGHGGGHGGGHQIGHFSDSDDDIEEGIKHVTAPILINSASHGYETTFLMASEEGGEYETVFFRRLDDEFNKVEKFYKEKVEEVMKEAVMLEKQMDALIAFRVKVEHPDGWPWEERTVEMTQLASDVANSAAAVAASTPAGARSMKVGAQAHMEAIQEGGSSKAGKSSDEEDDDDAEKEEDNGVSGEVSGDVRKMKAGRPPPIEVLDRVKFNHTKETPRSTIKSVLQASNLTELKFSRENLRKVEAKLRRAFVEFYQKLRLLKSYSFLNELAFSKILKKYDKITSRHASKSYMKMIDNSYLGSSDEVTRLVERVEATFIKHFSNANRSKGMNILRPKAKRERHRITFSTGFLGGCLFSLVVALFAIIRTRNILQEEGQKQYMNTMFPLYSLFGFVVLHILMYAGNIYYWRRYRVNYSFIFGFKHGTELGYRQVLFVGLSIGVFALLCILANLDMEVDPETKDYQALTELLPLFLLTGMFVVLVLPFNIFYRSSRFFFLTCLFHCLAAPLYKVTLPDFLVGDQLTSQVQALRSIQFYICHYGWGDYKHRINTCTESDAYNAFLFIVAVIPYVSRLLQCLRRLFEEKNPEQGYNGLKYFLTIVAVCLRTTYSVDEDNQFIWRILAGIFSAIAAIFCTYWDLVYDWGLLNRTSKNPWLRDKLLVPQKKVYFIAMILNILLRFAWLQTVLDFNFSFMH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be able to modulate cellular response to phosphate starvation, phosphate ion transport in a way that leads to a desirable outcome.2. For general function, the protein need meet that May transport inorganic phosphate (Pi). The designed protein sequence is ",
    "response#1": "PTLKASGSTAQANAMTRFVNAFERSCPGQTLNYTANGSGAGVSEFNGNQTDFGGSDSPLSRKEYAAAEQRCGSQAWNLPVVFGPIAITYNVNGLSSLNLDGPTTAKIFNGSIASWNDPAIQALNTGVALPAEPIHVVFRNDESGTTDNFQRYLDVASNGEWGKGIGKTFKGGVGEGAKGNDGTSAAVKSTEGSITYNEWSFASARKLNTAKIATSADPEPIAISVDSVGKTISGATIIGEGNDLVLDTVSFYKPAQPGSYPIVLATYEIVCSKYPDAQVGRAVKAFLN",
    "response#2": "PTLKASGSTAQANAMTRFVNAFERSCPGQTLNYTANGSGAGVSEFNGNQTDFGGSDSPLSRKEYAAAEQRCGSQAWNLPVVFGPIAITYNVNGLSSLNLDGPTTAKIFNGSIASWNDPAIQALNTGVALPAEPIHVVFRNDESGTTDNFQRYLDVASNGEWGKGIGKTFKGGVGEGAKGNDGTSAAVKSTEGSITYNEWSFASARKLNTAKIATSADPEPIAISVDSVGKTISGATIIGEGNDLVLDTVSFYKPAQPGSYPIVLATYEIVCSKYPDAQVGRAVKAFLK",
    "response#3": "PTLKASGSTAQANAMTRFVNAFERSCPGQTLNYTANGSGAGVSEFNGNQTDFGGSDSPLSRKEYAAAEQRCGSQAWNLPVVFGPIAITYNVNGLSSLNLDGPTTAKIFNGSIASWNDPAIQALNTGVALPAEPIHVVFRNDESGTTDNFQRYLDVASNGEWGKGIGKTFKGGVGEGAKGNDGTSAAVKSTEGSITYNEWSFASARKLNTAKIATSADPEPIAISVDSVGKTISGATIIGEGNDLVLDTVSFYKPAQPGSYPIVLATYEIVCSKYPDAQVGRAVKAFLN"
  },
  {
    "reference": "MAILQMENGSKLTDLQSIVQELAPLGITLNYWSIGDNPELHHLLSQDSLDDAQKETVLTHLDHYFNELASTGYTSRDLIVLHPEVPNLDTMLSKFDKIHTHADDEVRYIVAGEGIFGFVRPDGSQVELTVQAQEYINVPAGTQHWFILTPAKRVKAVRYFCGTAGWVPEYTDIQIRVGRQVAGV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway..2. The presence of Ni(2+) is necessary for the protein to function in the desired environment.3. The designed protein should have a binding site that is specific to the target Ni(2+) ligand.4. The protein must exhibit acireductone dioxygenase [iron(II)-requiring] activity. The designed protein sequence is ",
    "response#1": "PRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGGLLGVRDEMVLGVKDCAGPRSAAEMSEAEREALGIRERMPLGCEEGRNALEGDTELKGVLGEEFVERYLAVNRTLETVLVDKKETEEQRLTRWLRSMRAYYFDNLPGDQRAPHDYVPSRPVDASALAKMNVLSWHR",
    "response#2": "PRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGGLLGVRDEMVLGVKDCAGPRSAAEMSEAEREALGIRERMPLGCEEGRNALEGDTELKGVLGEEFVERYLAVNRTLETVLVDKKETEEQRLTRWLRSMRAYYFDNLPGDQRAPHDYVPSRPVDASALAKMNVLSWHGLCPRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGPRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGGLLGVRDEMVLGVKDCAGPRSAAEMSEAEREALGIRERMPLGCEEGRNALEGDTELKGVLGEEFVERYLAVNRTLETVLVDKKETEEQRLTRWLRSMRAYYFDNLPGDQRAPHDYVPSRPVDASALAKMNVLSWH",
    "response#3": "PRTLLRRITNTLESDFQTRFLVGFESEFILLKSTDPVAPVNIHDFAASTGLLTGTPEAAALQEIADSITAAGIALEMVHAEAAPGQYEVVTGPLTPLEAADALIHTREIIVQVAAKHGLHATFAPRPFMSSAGSSTHAHISVHSSDEEKPASALSQREFQFLAGLLAHLRGITALTLPTPASYKRVGDGLWSGGTYVCYGTENREAPIRLTNAASPASRNFELRFIDGTANPHLALAGVLAGGLLGVRDEMVLGVKDCAGPRSAAEMSEAEREALGIRERMPLGCEEGRNALEGDTELKGVLGEEFVERYLAVNRTLETVLVDKKETEEQRLTRWLRSMRAYYFDNLPGDQRAPHDYVPSRPVDASALAKMNVLSWHLS"
  },
  {
    "reference": "MLNSVQHLTKSRTYETLEQVQGDKLRLFTRPSCFAFREMASRLFLAYNGRRRGRLMGRDTQEKYFPVREMTDGERIRTVKEIFSTVTGKYDFLNHLLSLRRDIAWRRFAVRKMRFFKTQRFLDVATGTADLAVEACRRYPFLQVTGLDFIQAMLDVGLKKAAEKGLSNRIRFLRGDALVLPFPDRYFDVAAVAFGIRNIPDRLGALREMRRVVVPGGQVMVLEMTFPRNRIFDGFYHFYLHHILPNIARAFSKNPAAYQYLADSIMNFPSPEAFAGQMEEAGLIHVKRHRLDFGITYLHVGIKPQKA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be designed to catalyze the following reaction a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine.2. A protein with S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity, 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity, S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity, S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity functions is required to enable menaquinone biosynthetic process, ubiquinone biosynthetic process.3. The protein must exhibit the following characteristics: Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2).. The designed protein sequence is ",
    "response#1": "MIEAKNRMLHRVDQAAIEEGLRALEEGRVVIVCDDEDRENEGDLVISAERVTADIVNFMTREARGLLCAPMTAARAEELELEPMVQRNTDNHETAFTVSVDAAAGTSTGISAADRATTIRVLAAPDSRPEDLTRPGHVFPLRARPGGVLTRAGQTEAAVDLARLAGLRPAGVLCEIMHADGTMARVPDLRAFARRHGLKIITIADLIEYRRRTEKLVRCGRGDVEAPPTLHQASLCGAAVS",
    "response#2": "MTTAMPLDQTAPSSLPTRFGIRTAPRAELAALLEAERAELALASERFPHIRPAGDDPAAIEAELDRLTGHDAAFFRPKPGTPSLGGLPLIVAGCSDLRASRPGAFGRLFADRSVAVPAAAVAQVTAPDAAISSTRLWLGPEASSAWYDLMNNLMSFGIHHLRNVMVFGGEA",
    "response#3": "MKLQDILYKVSVNAVVGTTDVAVLEVSHDSRKAEKGAVFAAIKGATFDAHKFVPQVAAAGAAAVIVSREVPVPQVIVPDTRIALSLIANAFYDHPAQALKLIGVTGTNGKTTVTYLIDSIFKAAGHKCGLISTVNYRIGDKVWPAANTTPESLDLQRLLAEMVDAGCEYCFMEVSSHAIAQGRVNGCDFDVAAFTNLTQDHLDYHKTMEAYFAAKKRLFTEYLGCDKRKTAVINADDAAGRYIADETKARVLTYAIKKPADLRAVDIQMTAKGTHFVLHTAAGLTEVKSNLIGRFNVYNTLAAVGAGIALEIPAETIKSALEAVPGVARVCKPGGKLMVLEFGHETYQILDDAYNASPESMTAAIDGLRQMKGNRKIRVLKPGGRLLILEFGHEKTQTFANYEKVGEVAARLGLDELITIGDKDAIGYLARGLKSGAFSEEQVRIHLDKEEAIAYLKTQTKPGDVVLLKGSNRFERMEEVISVLEKG"
  },
  {
    "reference": "DCPTRCPTTCANGWECCKGYPCVNKACSGCTHGK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a specific function within the extracellular region. The designed protein sequence is ",
    "response#1": "PGDGGPPGQPGPDGPPGAPGNAGEDGKPGDQGPRGPPGIPGAPGQPGRPGPPGEPV",
    "response#2": "PGDGGPPGQPGPDGPPGAPGNAGEDGKPGDQGPRGPPGIPGAPGQPGRPGPPGEPL",
    "response#3": "PGDGGPPGQPGPDGPPGAPGNAGEDGKPGDQGPRGPPGIPGAPGQPGRPGPPGEPF"
  },
  {
    "reference": "MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWSAWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDLFQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLKEHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGTDEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWNKRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVPFQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALATGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAIVKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMIFGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSSTPTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAVLKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRHYLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQRICKELGKNLPFTRLDELAHNALVKGLI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein have enhanced a long-chain 2,3-saturated fatty acyl-CoA + H(+) + oxidized   [electron-transfer flavoprotein] = a long-chain (2E)-enoyl-CoA +   reduced [electron-transfer flavoprotein].2. The protein should be optimized for activity in fatty acid beta-oxidation pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The presence of FAD is necessary for the protein to function in the desired environment.4. The designed protein should have medium-chain-acyl-CoA dehydrogenase activity, flavin adenine dinucleotide binding, acyl-CoA dehydrogenase activity to facilitate fatty acid catabolic process, fatty acid beta-oxidation using acyl-CoA dehydrogenase.5. For general function, the protein need meet that Catalyzes the dehydrogenation of acyl-coenzymes A (acyl-CoAs) to 2-enoyl-CoAs, the first step of the beta-oxidation cycle of fatty acid degradation The designed protein sequence is ",
    "response#1": "VLTRVKQFMKQHVFPAEKEVAEYYAQNGNSAEKWEHPLVIEKLKEMAKAEGLWNLFLPAVSGLSQVDYALIAEETGKCFFAPDVFNCQAPDTGNMEVLHLYGSEQQKQQWLEPLLRGARLFCDNDAQAAIGMTEPSAGSDLQGIRTNAKKDGDDYILNGSKVFITNGYMSDVCIVVAITNTEAKSVAHGISLFLVEASMPGFIKGKKLQKIGLKAQDTAELFFEDCRIGRETNVTNAALIGDARNKGFYYLMNELPQERLLIADMAIASSEFMFEETRNYVTQRKAFGKTIAHLQTVQHKLAELKTEICVGRSFLDTCLQLHTEKRLDSQTASMAKYWASDLQNSVATRCLQLHGGWGYMWDYPIAKAFVDSRVQPIYGGTNEIMKELIARSRLGDS",
    "response#2": "IRCHPFVLDEMAAAQSNDVAAFDKALFGHLGHVGSSMVRSFWLGLTNGRTSATPVKDSTRRYYQQLNRLSANLALLSDVSMGVLGGSLKRRERISARLGDILSQLYLASATLKRYEDEGRQKEDLPLVHWGVQDALYQAEQALDDLLRNFPNSVIAGLMRVVVFPLGRVHRAPSDHLDHQLAQLLQVPSATRSRIGRGQYLTPSEFNPVGLLEAALLDVIAAEPIHKRLSKEAGKSLPFTRLDQLAKHALAEGKIRCHPFVLDEMAAAQSNDVAAFDKALFGHLGHVGSSMVRSFWLGLTNGRTSATPVKDSTRRYYQQLNRLSANLALLSDVSMGVLGGSLKRRERISARLGDILSQLYLASATLKRYEDEGRQKEDLPLVHWGVQDALYQAEQALDDLLRNFPNSVIAGLMRVVVFPLGRVHRAPSDHLDHQLAQLLQVPSATRSRIGRGQYLTPSEFNPVGLLEAALLDVIAAEPIHKRLSKEAGKSLPFTRLDQLAKHALAEGKE",
    "response#3": "SEEHDMFRNSVRRFMQEEVLPNHGQWEKDGNVSKEVWLKAGENGLLGVNTPAELGGVGGDFLMTNITWEEQMYVNCTGPGFTLHSDIVMPYITHYGTKEQIEKFIPKMTSGCSVASAIAMTEPGAGSDIQGVKTYARKDGSDWILNGNKVFISNGWMSDLVVVVAVTNRDAKTAAHGISLFLVENGTQGFHKGRKLEKIGLKAQDTAELFFEDCRIGKNCACFAMTELGHGSNVRGIETVTTYDPKTEEFVINTPCESAQKYWIGGAANHATHTIVFSQLHINGTNQGVHAFIAQIRDQDGSICPNIRIADCGHKIGLNGVDNGRIWFDNKGFYYLMNELPQERLLIASMGQANNEWMFEETRTYVKNRKAFGKTLSKLQTIQHKLAELKTEICVGRAFIDQCLKTHSQRGLDSSTASMAKYWITDLQNKVATECVQLHGGWGYMLEYPISKAFLDARVQPIYGGSNEIMKELIARQLMARA"
  },
  {
    "reference": "MSSIGTGYDLSVTTFSPDGRVFQVEYATKAVDNSGTVVGIKCKDGIVLGVEKLVTSKMMLEGSNRRIHSVHWHSGLAVAGLAADGRQIVSRAKSEAASYEKVYGEPISVKELADRVASYVHLCTLYWWLRPFGCGVILGGYDRDGPQLYMIEPSGVSYKYFGAALGKGRQAAKTEIEKLKLSELTCREGIVEVAKIIYGVHDEAKDKAFELELSWICDESNRQHQKVPADLLEQAKVAAQAALEEMDAD",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH2. The protein should be designed to localize specifically to proteasome core complex, alpha-subunit complex in order to perform its role in proteasomal protein catabolic process more effectively. The designed protein sequence is ",
    "response#1": "VEKAELRLAIADSPQKFETNLQTFLPPLLLKLASPHASVRTAVFSALKNLISRINTLPQVQLPVRALIVQAKEPNLAAQQDSTNVRLYSLLLASKGIDRLSLQDRQQLLPLVVSNISCLTGTVAARMFHILLKLILEWVAPQESSHEQEEFVQFLQLDNDGFSFLMRQFTRFFLLVPSKQVQVSQQPLSRGYTCPGLSLTDVAFFTYDAGVTFNKEQLNKFKKAIFQFVCRGMAATQTIEQSPRMIELMEFLCVVSTDSTNLSDDAAQFMKRFPMPYENEEFITFLQTLYIGNTANGRPPVKAILQEKILSILNRSHFATTKAECISLICSIGLHSSEYKLRSLTLSFIRHVAKLNYKNLNPASSSPSSTDFSTCIVSLIRNNLHAEGWPKLQLGPQTPAFNTAILQRQLQYETLGDILKRDFELVSDLSYIEFLFESLKNDLPQFRSSIQESLLSLVGHLSILPQQSKLKLKNLLRKNLSIDEQQREDNNDAVNSIMALKFVSIKFTNAAFPFHDPEARLFNIC",
    "response#2": "VEKAELRLAIADSPQKFETNLQTFLPPLLLKLASPHASVRTAVFSALKNLISRINTLPQVQLPVRALIVQAKEPNLAAQQDSTNVRLYSLLLASKGIDRLSLQDRQQLLPLVVSNISCLTGTVAARMFHILLKLILEWVAPQESSHEQEEFVQFLQLDNDGFSFLMRQFTRFFLLVPSKQVQVSQQPLSRGYTCPGLSLTDVAFFTYDAGVTFNKEQLNKFKKAIFQFVCRGMAATQTIEQSPRMIELMEFLCVVSTDSTNLSDDAAQFMKRFPMPYENEEFITFLQTLYIGNTANGRPPVKAILQEKILSILNRSHFATTKAECISLICSIGLHSSEYKLRSLTLSFIRHVAKLNYKNLNPASSSPSSTDFSTCIVSLIRNNLHAEGWPKLQLGPQTPAFNTAILQRQLQYETLGDILKRDFELVSDLSYIEFLFESLKNDLPQFRSSIQESLLSLVGHLSILPQQSKLKLKNLLRKNLSIDEQQREDNNDAVNSIMALKFVSIKFTNAAFPFHDPEARLFNIL",
    "response#3": "VEKAELRLAIADSPQKFETNLQTFLPPLLLKLASPHASVRTAVFSALKNLISRINTLPQVQLPVRALIVQAKEPNLAAQQDSTNVRLYSLLLASKGIDRLSLQDRQQLLPLVVSNISCLTGTVAARMFHILLKLILEWVAPQESSHEQEEFVQFLQLDNDGFSFLMRQFTRFFLLVPSKQVQVSQQPLSRGYTCPGLSLTDVAFFTYDAGVTFNKEQLNKFKKAIFQFVCRGMAATQTIEQSPRMIELMEFLCVVSTDSTNLSDDAAQFMKRFPMPYENEEFITFLQTLYIGNTANGRPPVKAILQEKILSILNRSHFATTKAECISLICSIGLHSSEYKLRSLTLSFIRHVAKLNYKNLNPASSSPSSTDFSTCIVSLIRNNLHAEGWPKLQLGPQTPAFNTAILQRQLQYETLGDILKRDFELVSDLSYIEFLFESLKNDLPQFRSSIQESLLSLVGHLSILPQQSKLKLKNLLRKNLSIDEQQREDNNDAVNSIMALKFVSIKFTNAAFPFHDPEARLFNII"
  },
  {
    "reference": "MKAQVRTLTGEIAHEIDLPEIFREEYRPDLIKRAVLALQSTRFQPHGTNPYAGMRTSAESWGSGRGAAQVPRLKNGSRVARVPQATGGRAAHPPKVEKILVKEINRKEKRKALRSAVAASTYPDLVRERGHLFEGDLPLVFEDRFEEVTRTGDVIAALTALGVYADVERAKGSRKVRAGRGTMRGRRYKQRKSVLIVTGGEPLRAARNLAGVDAVAVDQLNAELLAPGTQAGRLTIWTESAIRRLEEFS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be expressed specifically in the ribonucleoprotein complex The designed protein sequence is ",
    "response#1": "LGKTVAFGEYGLEALESHWITNRQIEAARVAMTRYMKRGGKVWIRIFPQKSYTAKGVGVRMGSGKGAPAGWVAVVKREKIMFEIGGVSEETAREAMRLAASKLPIKCKFG",
    "response#2": "GGKTVAFGEYGLEALESHWITNRQIEAARVAMTRYMKRGGKVWIRIFPQKSYTAKGVGVRMGSGKGAPAGWVAVVKREKIMFEIGGVSEETAREAMRLAASKLPIKCKFG",
    "response#3": "GGKTVAFGEYGLEALESHWITNRQIEAARVAMTRYMKRGGKVWIRIFPQKSYTAKGVGVRMGSGKGAPAGWVAVVKREKIMFEIGGVSEETAREAMRLAASKLPIKCKFG"
  },
  {
    "reference": "MSKKTVANLSAADLAGKRVLVRVDFNVPLDDQGKITDDTRIRAALPTIQDLTSKGAKVILSSHFGRPKGETFAERVKDKFRLTPVAARLSELLGKPVPKPNDCIGEEVKAQVAAMQNGDVLLLENVRFHPGEEANTPEFAQELASVADLYVNDAFGTAHRAHASTEGVTRYLRPSVAGYLIEKELQYLQSAIENPQRPLAAIVGGSKVSSKIGVIETLLEKVDKLLLGGGMIFTFYKARGLNVGKSLVEEDKLELARTLEAKAKERGVALLLPTDVVVADAFAADANAQTVSVESIPDGWMGLDIGPDAVKTFQEALSDCKTVIWNGPMGVFEFDKFAVGTEAIAHSLAGLTGKGASTIIGGGDSVAAVEKVGVAEQMSHISTGGGASLELLEGKELPGIVALDDA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. We require a protein that can specifically bind to the metabolites produced in the pyruvate from D- glyceraldehyde 3-phosphate: step 2/5 pathway.2. The protein must be able to ATP binding.3. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate, ATP binding. The designed protein sequence is ",
    "response#1": "MTYN",
    "response#2": "GMI",
    "response#3": "MPFSLN"
  },
  {
    "reference": "MRRVPDVVPRQALPIVLVGLPGAGKTSVSRELARLTGMPSIDTDSEVRRHTRLSIPEIFAQRGEAGFREVEHRAIAHTLQIAHGIVALGGGAVLHRGTRELLRSHTVVHLLVEPDEAVRRVTGGEDRPLLGNDPDAKMREFHAQRAALYRDVTTVAVETTGRAPAEIAEEIVEKIRQGIGRVSQVKVPGNRPYSVVLGHGLNGRIAHLVGGTARRVLLVHAPALADRAEALAAKLTRSRHTHHDVKVIRHVLPAGEAAKSLTVTGQLWDVMGAEQLGRQDVVVALGGGATTDQGGFAAATWLRGIDVVQVPTTVLAMVDAAVGGKTGINTSAGKNLVGAFHPPRGVICDLDSLETLPRPDRRAGLAEVLKCGLIADPVILERVAQDPQALVDGPVVAMREIMERAIEVKAKIVGKDLRESGLRELLNYGHTLAHAIERHSDYQWRHGDAVAVGCVFAAALAEELGVAAAGMLDQHREIFASVGLPVTYPREAFADLREIMAQDKKVRDRSLRFVLLRCLAKPQVLVVRDEAALQRAYAAVAE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein with 3-dehydroquinate synthase activity, ATP binding, shikimate kinase activity is needed to enhance phosphorylation, amino acid biosynthetic process, chorismate biosynthetic process, aromatic amino acid family biosynthetic process in a specific context.2. The designed protein have 3-dehydroquinate synthase domains with specific sequence motifs that are crucial for its activity.3. The stability of the protein-NAD(+) complex should be optimized to maximize enzymatic activity.4. The ATP, Mg(2+), Zn(2+), NAD(+), substrate binding site should be located in a region of the protein that is accessible to the ligand.5. The protein design should consider the endogenous proteins and enzymes present in the chorismate biosynthesis, Metabolic intermediate biosynthesis pathway to avoid interference with native metabolic processes.6. The protein must exhibit the following characteristics: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.. The designed protein sequence is ",
    "response#1": "MLVPDAEAGKTIQVASGLWARLGDAGFTRSDAIVGLGGGAATDVAGFVAATWMRGIRYVNCPTSLLAMVDASTGGKTGINTDAGKNLVGSFYTPAGVLADTSTLSTLPNDVALHSGRLSLPNGWAEIVKHSMIECTATATPEPVLLPWLEGRTPVGGLGEPGELAALIAHNIRLKAAVVRADEREAGLRRILNYGHTLGHAIEAAGYRYLHGEAVALGMRAAARLAHRLGLCDADLVARQDALLDRLDLPRTYDGEWEPVREHLLRDKKADEILAELGLGEVAKSGFILDGEILKILSDSNNAKLLREFDGDQLENNNELHELLVELIERTVRVKAYHVSSDLKEQGLREFLNYGHTLGHAIEQIEHFRWRHGNAVAVGMVFAAELAQILGYLDTQDVSYHRSLLDSLGLPTSWQSREDADSAFERVLNLMHRDKKLNADSL",
    "response#2": "MLVPDAEAGKTIQVASGLWARLGDAGFTRSDAIVGLGGGAATDVAGFVAATWMRGIRYVNCPTSLLAMVDASTGGKTGINTDAGKNLVGSFYTPAGVLADTSTLSTLPNDVLVAQDKICGPNKIVAGYAEIVKAGLIADPALYEWCEAHGAALLGGDQGLQEEAIARAVAFKAQVVMADPREQAKRDGRALLNLGHTFAHAFEAETGYGEALLHGEAVAIGMVCAFDLSARLGYCDAGIVPRIAAHLDEVGLPHRIGGWNAEALLGHMQSDKKGYAEIVKSGLIGDEALFTWCEKHGAAVLSQEPGALAEAVEKACAFKAAVVTKDEFERSSEGGRALLNLGHTFGHALEAELGYDGRLLHGEAVSIGLRLAFLLSVRLGYCSGADLDRVTRHLESNGMPVRISDIGEHFSAETLLHHMTRDKKLAENLSFGLGEVAKSGFILDGEILKILSDSNNAKLLREFDGDQLENNNELHELLVELIERTVRVKAYHVSSDLKEQGLREFLNYGHTLGHAIEQIEHFRWRHGNAVAVGMVFAAELAQILGYLDTQDVSYHRSLLDSLGLPTSWQSREDADSAFERVLNLMHRDKKLDVFVHEVAGLGEVVKCGFIADPAILDLVEGVAPEEITADSPVLRELVERAIGVKIAVVVTDLKETGGVDGHPGREALNYGHTLGHAIERAEGYRIRHGEAVALGCVFVAELARRTGVLSADVAARHRSVLARVGLPTTYAGAGFEELLAAMRVDKKLKD",
    "response#3": "MLVPDAEAGKTIQVASGLWARLGDAGFTRSDAIVGLGGGAATDVAGFVAATWMRGIRYVNCPTSLLAMVDASTGGKTGINTDAGKNLVGSFYTPAGVLADTSTLSTLPNDIVPAGEEAKSLDWARLLYDQLLAAGAGRDAWVCALGGGAVGDLAGFVAATYMRGLPFVQLPTTLLAQVDASVGGKVAVNHARVKNLVGAFYQPRLVVADPDTLLSLPERAYSRIVSENLYIGYAEVAKYGLIDNAEFFTWLEANWRDVFAGGPARLKAIATSCAAKARVVAADEMEQGERALLNLGHTFGHALERLTHYDGERLVHGEGVAIGMACAFRFSRDLGLCPEADAARVERHLEAVGLPTRIGALRGLNADAKAILEAMRQDKKLRSLGASIHRVGLGEVAKSGFILDGEILKILSDSNNAKLLREFDGDQLENNNELHELLVELIERTVRVKAYHVSSDLKEQGLREFLNYGHTLGHAIEQIEHFRWRHGNAVAVGMVFAAELAQILGYLDTQDVSYHRSLLDSLGLPTSWQSREDADSAFERVLNLMHRDKKLWE"
  },
  {
    "reference": "MHIVTVSEMKELEDQAGQKYGLTSPILMNNAGQSAAEIFKSKLLPHQKLTGLRILFLIGPGNNGGDGLVMADHLQKAGVIISCYHWKEHTLNIQGKECIEADTASILETQIQHADYIIDALLGTGRSRSMADDMRSLLARVKQERTQRPHLRIIAIDLPSGLNADTGEVDPGSIEANRTITLACPKQGFFFFPGRDYIGELSVGSIGLPAELESPLRTEMLSGKLVHTLLPERPLNSNKGTFGKAMLLCGSLPYPGSAYLAGSAAGRVGGGLVTLAVTEKMLSFYASSFHEATFVILPENDRESFERSNALIEKLEGYKALLIGPGLGQSPNTREVILQVLEYVRSLPQEKRPHLVVDADGLNNLSALERWWTLLPKGTIITPHPGEMGRLSGGIKVSGGNFERLELARQKAKEWQVNLVLKGACTILADPQGRTRINWWGNPALATAGTGDVLAGMITGFLAQKMDPFDAASVAIYLHTATADLVTEERHIGRAGLLASDLLPSIPNALVNTGDAEL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.2. The K(+), AMP binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein should be optimized for metal ion binding activity in order to improve nicotinamide nucleotide metabolic process.4. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "MLVAGAPETMGAALLTSRACLYAGAGLTSACIPATGLTALNASLPEVMAVVRSEQGLPTSVDWEKYQAIGIGPGLGTGPGSLSLLRVVLKNFHKPIVLDADALNLIAHNYELIQQVPEHSVLTPHMKEFDRLFGDHQDWWSRIETGIDRATTLGCTIVLKNRYTIIFTPQGMCLFNPTGTPAMATGGSGDALTGIITSFLAQGYEPHEAAFIGTYVHGAAGERVWPVRRRAAHKGDFGSVFILGGSINMPGAPELAARAALRAGAGLVFKSVKPGGSVSSWPEVILHHGASFSDFSQKIRDCSCLVFGPGLGLDREAKALVEEALGVISEIQMPSVIDADALTLIAEMLKVGRTFSLPSAVITPHPGEAARLLGVDTAAVQHDRYSAARKLSELLNCTALLKGAGTVIYGRGRGIVNASGNPYMATAGSGDVLSGILGAFLAQDLAGLYAAAAAAFLHGKAGDLAYHEIGGPLVASDLIAYLPKAWGREALDGWLSPRRRNAHKGDSGHVLCVGGDTGHGGAVMLAADAALRCGAGLASVATRSAHVPGLLARRPEAMARGVDGAAALAPLLARADVVALGPGLGQGSWGRALYGAVLAAGLPLVLDADALNLLAAAPRELAADTILTPHPGEAARLLGMETTQVQADRFAAAARLVERYGCVVVLKGAGTVVAAPGQSPRVVAAGNPGMAVGGMGDLLTGCIAALRARGMPAFEAASLGALLHALAGDAAARDGETGLLPSDLLPWLRALAS",
    "response#2": "MLVAGAPETMGAALLTSRACLYAGAGLTSACIPATGLTALNASLPEVMAVVRSEQGLPTSVDWEKYQAIGIGPGLGTGPGSLSLLRVVLKNFHKPIVLDADALNLIAHNYELIQQVPEHSVLTPHMKEFDRLFGDHQDWWSRIETGIDRATTLGCTIVLKNRYTIIFTPQGMCLFNPTGTPAMATGGSGDALTGIITSFLAQGYEPHEAAFIGTYVHGAAGERVGVIDGIKKWPVRRRAAHKGDFGSVFILGGSINMPGAPELAARAALRAGAGLVFKSVKPGGSVSSWPEVILHHGASFSDFSQKIRDCSCLVFGPGLGLDREAKALVEEALGVISEIQMPSVIDADALTLIAEMLKVGRTFSLPSAVITPHPGEAARLLGVDTAAVQHDRYSAARKLSELLNCTALLKGAGTVIYGRGRGIVNASGNPYMATAGSGDVLSGILGAFLAQDLAGLYAAAAAAFLHGKAGDLAYHEIGGPLVASDLIAYLPKAWQKL",
    "response#3": "MLVAGAPETMGAALLTSRACLYAGAGLTSACIPATGLTALNASLPEVMAVVRSEQGLPTSVDWEKYQAIGIGPGLGTGPGSLSLLRVVLKNFHKPIVLDADALNLIAHNYELIQQVPEHSVLTPHMKEFDRLFGDHQDWWSRIETGIDRATTLGCTIVLKNRYTIIFTPQGMCLFNPTGTPAMATGGSGDALTGIITSFLAQGYEPHEAAFIGTYVHGAAGERVGVIDGIKKWPVRRRAAHKGDFGSVFILGGSINMPGAPELAARAALRAGAGLVFKSVKPGGSVSSWPEVILHHGASFSDFSQKIRDCSCLVFGPGLGLDREAKALVEEALGVISEIQMPSVIDADALTLIAEMLKVGRTFSLPSAVITPHPGEAARLLGVDTAAVQHDRYSAARKLSELLNCTALLKGAGTVIYGRGRGIVNASGNPYMATAGSGDVLSGILGAFLAQDLAGLYAAAAAAFLHGKAGDLAYHEIGGPLVASDLIAYLPKAWQKL"
  },
  {
    "reference": "MHEYSIVTALIEECERHAFANNASKVSRVEIKLGILSGVEPELLRTAFETFKLEGICREANLVMNIQPLVLRCLDCGQSTEHSERSVICSHCQSGQTKVLDGEDMMLMQLELEQA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have a high affinity for Zn(2+).2. The protein should be able to modulate protein modification process in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "RNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPSSPATKNPCNIHYSPNDEDKGMVLPGTKCADGKACSNGRCVDC",
    "response#2": "RNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPSSPATKNPCNIHYSPNDEDKGMVLPGTKCADGKACSNGRCVDC",
    "response#3": "LPTTTIGSFPQVAGVRSARRSFKQGRISEEQYRAEMQRYIREAIAQQEALGLDVLVHGEPERNDMVEYFGEQLAGFCFTENGWVQGYGSRCVKPPIIYGDVERLKPMTMEWTTYAQQQTNRPLKGMLTGPVTMLCWSFVRDDQPRSATCKQIALAIRDEVQDLEQAGIKIIQVDEAALREGLPLRKSAWREYLGWAVDAFRLTASGVADSTQIHTHMCYSEFNHIAEWIAKMDADVISIEASRSNMELLGVLESFTYPNDIGPGVYDIHSPRVPSVAEMVELLRKASEVIPLERLWVNPDCGLKTRAWPETLASLRNMVAAAG"
  },
  {
    "reference": "MKQQEVRQRAFAMPLTSPAFPPGPYRFVNREYMIITYRTDPAAIEAVLPEPLQMAEPVVRYEFIRMPDSTGFGDYSESGQVIPVTFRGERGSYTLAMFLDDQPPLAGGRELWGFPKKAGKPRLEVHQDTLVGSLDFGPVRIATGTMGYKYEALDRSALLASLAEPNFLLKIIPHVDGSPRICELVRYHTTDVAIKGAWSAPGSLELHPHALAPVAALPVLEVLSARHFVCDLTLDLGTVVFDYLRQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Catalyzes the conversion of acetoacetate to acetone and carbon dioxide..2. The target protein must exhibit helix-strand-strand-helix-strand-strand-turn-strand-strand-helix-strand-strand-strand-strand-helix as its primary conformation.3. The designed protein must be optimized for acetoacetate decarboxylase activity.4. The Schiff-base intermediate with acetoacetate of the protein must be engineered to enable effective catalysis of acetoacetate + H(+) = acetone + CO2. The designed protein sequence is ",
    "response#1": "DPAQFEIGFQRTNDILDEGMDVFVRSCRCAMGVAGDSLVAIMTADGDIVNGSCGTYLHAVIPPLIIKYILETYGDEIRDGDLWFANDAVYGGVHNPDQMVCMPVYYEGKLVAWTAALVHTTETGAIEPGGMPVSATTRFEEGMNLPPMRIGENFKLREDVVSMFVAFGLRAPSMIAVDLKARCTTADRVRTRIIELCEREGADYVTGLFRKMLQVAEAGARELIEQWPDGKYRCVTFSDAVGLKQGLVRSCYMTLEKKGDRMLVDLSETGPETPSPYNAHPQAAIAHFSNYIYEYLFHSLPISNGTFANIDFKFGKNTCLSPDPRAATSCSVMISTGVMSAVHNACAKAMFSTSLWKQSGASMGNGGNALVLAGQNQWGSSFADMLAYSINTEGQGARPTEDGMDAFGFPWCVFGRAPNTESVENEFPLLVPLSNHWKDSCGHGKYRGGVGTAQVWVAHHVPELYMMAIADNTKLQTPQPLFGGYAPCTVPGIGIRNANIKELMAEGSDKIKLDVETLLAERTIDGKYEIEFQGRSVRPYSNGEVVTFAFSCGGTGYGDR",
    "response#2": "DPAQFEIGFQRTNDILDEGMDVFVRSCRCAMGVAGDSLVAIMTADGDIVNGSCGTYLHAVIPPLIIKYILETYGDEIRDGDLWFANDAVYGGVHNPDQMVCMPVYYEGKLVAWTAALVHTTETGAIEPGGMPVSATTRFEEGMNLPPMRIGENFKLREDVVSMFVAFGLRAPSMIAVDLKARCTTADRVRTRIIELCEREGADYVTGLFRKMLQVAEAGARELIEQWPDGKYRCVTFSDAVGLKQGLVRSCYMTLEKKGDRMLVDLSETGPETPSPYNAHPQAAIAHFSNYIYEYLFHSLPISNGTFANIDFKFGKNTCLSPDPRAATSCSVMISTGVMSAVHNACAKAMFSTSLWKQSGASMGNGGNALVLAGQNQWGSSFADMLAYSINTEGQGARPTEDGMDAFGFPWCVFGRAPNTESVENEFPLLVPLSNHWKDSCGHGKYRGGVGTAQVWVAHHVPELYMMAIADNTKLQTPQPLFGGYAPCTVPGIGIRNANIKELMAEGSDKIKLDVETLLAERTIDGKYEIEFQGRSVRPYSNGEVVTFAFSCGGTGYGDL",
    "response#3": "DPAQFEIGFQRTNDILDEGMDVFVRSCRCAMGVAGDSLVAIMTADGDIVNGSCGTYLHAVIPPLIIKYILETYGDEIRDGDLWFANDAVYGGVHNPDQMVCMPVYYEGKLVAWTAALVHTTETGAIEPGGMPVSATTRFEEGMNLPPMRIGENFKLREDVVSMFVAFGLRAPSMIAVDLKARCTTADRVRTRIIELCEREGADYVTGLFRKMLQVAEAGARELIEQWPDGKYRCVTFSDAVGLKQGLVRSCYMTLEKKGDRMLVDLSETGPETPSPYNAHPQAAIAHFSNYIYEYLFHSLPISNGTFANIDFKFGKNTCLSPDPRAATSCSVMISTGVMSAVHNACAKAMFSTSLWKQSGASMGNGGNALVLAGQNQWGSSFADMLAYSINTEGQGARPTEDGMDAFGFPWCVFGRAPNTESVENEFPLLVPLSNHWKDSCGHGKYRGGVGTAQVWVAHHVPELYMMAIADNTKLQTPQPLFGGYAPCTVPGIGIRNANIKELMAEGSDKIKLDVETLLAERTIDGKYEIEFQGRSVRPYSNGEVVTFAFSCGGTGYGDVIVSAARTPFGKFGGVLKEVKAAELGGIVMKEALQQAGVSGDDVEGNVMGMVVQAGSGQIPSRQAARLAGMPWSVPSETLNKVCASGLRAVTLCDQMIRAQDADILVAGGMESMSNIPYAVPAGRWGARMGDGELRDLMVYDGLTCAFDEVHMAVHGNTAAKEYAISRREQDEWALRSHARAAKAADEGKFQDEIVPVNWIGRKGKPNVVDKDEAIRRDTSLDQLAKLAPIYASDGSITAGNAPGVNDGAGAFVLMSE"
  },
  {
    "reference": "MSRRRILLASRNPDKLSEIRDLLAVVPIDIMSPDELGIAEDPAEAGVECWDGFAANALAKASWFRARSGLPTIADDSGLCVDALNGGPGVHSRRFAPVDMIDDDGQHAANNRHLLTLLEGMPEKERGAYFCCALALLDDRFRVVTIGRVEGTIATAERGTGGFGYDPLFVVPEFGRTFGELSADVKAELSHRARAVQAMRSWLG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..2. The designed protein must be optimized for metal ion binding, ITP diphosphatase activity, dITP diphosphatase activity, nucleotide binding.3. The protein requires Mg(2+) to activate a critical catalytic site.4. The designed protein should have a high specificity for Mg(2+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MQIVLATHNPGKAREFASLVGPHGLELVGVAEFGLPEPAETGTTFVENALAKARHASAATGLPALADDSGLAVDALGGAPGVRSARYAGEAATDEDNNALLLAELAGVADRRARFVCALALVRGADAEPVIAEGRWEGVILEAPRGANGFGYDPLFVPSGHDRTAAELTPDEKNAISHRGQAFRALVAQLPSLLA",
    "response#2": "MPEIRLILATRNEKKLKEIRNIL",
    "response#3": "MKLVLATRNAHKTREMQQILEPLGIEVASTLALDAFPEAGEVGLTFAENALEKARHAAKATGLPAVADDSGLCVDVLGGAPGLFSARYAGVHGDDGANNALLLAQMRDVPDEHRGAHFFCAIALVQPDGSEQVVEARWPGRLLREPRGENGFGYDPLFIPDGEEGSCAQMSAEDKNAISHRARAFRALRPILAALLG"
  },
  {
    "reference": "MQLSNVLHGLDYKIKQGDLNISIDEIIYDSRIKTNEGLFVAIKGFSSDGHKYISSAISNGAKVVVIQDDVEVEGDVTVIKVDDTRYTIAILARNFYNNPSQSFNLVGVTGTNGKTSTTFLLGQMLEAHKQKVGIIGTIENRIGDKVLPTERTTPESLDLQKLFAQMNDENVDSVVMEVSSHALDLHRVTGCQFDFGIFTNLTQDHLDFHKDMESYAHAKAKLFGMSKVSIINKDADFSELMSQSASGDLITYAIDQEADYQAINLDIQATGVNYTLLYNGNSYDVAVPISGKFTVYNTLGAIVVAHKMGLDLEEIIQTLATVKGVPGRIQSFNSKNGYSVIVDYAHTPDGLENVLGAIEQFVKGKIITVFGCGGDRDNKKRPLMGAVVAKNSDYTIITSDNPRSEEPESIIDQIEVGVKEHTQTYIRQSDRKKAIHQAMEMAKEGDVVLVAGKGHETYQHLKDGIIHFDDSEVVKEFLEGEL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a low Km value for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.2. The protein should be able to bind ATP ligand in a variety of conditions3. A protein should be able to manipulate the activity of enzymes in the peptidoglycan biosynthesis pathway, either by activating or inhibiting their function.4. The designed protein should have magnesium ion binding within cytoplasm to regulate cell cycle. The designed protein sequence is ",
    "response#1": "MAGVTGTDGKTSVAHILAQALSEPGAPAGYIGTLGFGPVDALQPGTHTTPDAIGVQGLLDAFRARGTRQVAMEVSSHALDQHRVAGVRFRTAVFTNLSRDHLDYHGDLETYAAAKRRLFQCPDLEAAIINVDDPVGEALAESLAGGVSVTGFSLIPERAGEPDLLVVERITFDSDGMHLDLVTPQGRAELHSRLLGRFNAANLLAALGALHALGEPLEATLARLAAVKPPPGRMELVSGTAPVRGVVDYAHKPEAISAVLDSLQETTPGRVIAILGAGGDRDASKRALMGRAAAASADLVMITDDNPRTEDPASIRAQVASAVQAVPCRNISGREVAVREAVAMAAPGDTIALLGKGADAFQEVQGERLPYADLDVVRQALAAL",
    "response#2": "MKLISNIAFHSGRVEPGGLFVAVDGFRVSGADYAGEAVSRGAVAVAAGRGAAAVALPQTVARVEVDDARKFLALAADRFYGHPSREMSLVAITGTNGKTSVSFMVESVLRIAEGHKVGIIGTLGAYLGDEDLPATHTTPESVELHRIMAQMADAGCKYVVMEVSSHAIDQGRVYGCDFDAAVFTNLTRDHLDYHKTMEAYFQAKAKLFTLLGAEKKSQRFAVINGDDPAARYMAGSTKAQVITYSLEHPADLRAENIHISASGTSFHVSTPAGRFDIELKMVGKFNVYNALAAFGASMLEGLTLAQIKEGLEAVSGVPVAITGTNGKTSVSFMVESVLRIAEGHETYQIIGTEKHHFDDREEAQAALYGRMG",
    "response#3": "MRVSGLALDSRQVRSGDAFFALRGTRGHGIEFVRGAVQRGATVVLAEAPAPQDAEDPGVPVVWIDGLHTFVGEIAARFFGRPSDRLSIVAITGTNGKTSVSFMVESVLRIAEGRAGMIGTLGYGMPGLLGATHTTPDAVEIQSMLARMRAEGAEWAAMEVSSHALAQHRVDGVAFDVAVFTNLTQDHLDYHHTMEEYFAAKRKLFTPEHLKIAVINGDDPYGRRLVDQLGDRITTYSVNGAADVRVRPLNWSSSGFELHLDIGGIRERVSLPLMGRFNVYNALAAVTAALVEGIPLAEIERALAGVSGVPGRMELLPTGASRPAAMIDYAHTPDALESALRAARGHCWGSLWVVFGCGGDRDRGKRADMGRIAETLAEHVVLTDDNPRSESSAAIIADIASGLGRLQPTVLNDRAEAIRFALHSSGPGDLVVIAGKGHEDYQILGTEKIHFDDREHARAALIERLV"
  },
  {
    "reference": "MFGNLFEEDYSSVSSSQYGRGKKLKTKGLEPPAPREFTNLSGIRNQGGTCYLSSLLQTLHFTPEFREALFSLGPEELGSLEDKDKPDAKVRIIPLQLQRLFAQLLLLDQEAASTIDLTDSFGWTNDEEMRQHDVQELNRILFSALETSLVGTSGHDLIHRLYHGTIVNQIVCKECKNISERQEDFLDLTVAVKNVSGLEDELCNMYVEEEIFDYDNLYHCGTCDRLVKAAKSAKLRKLPPFLTISLLRFNFDFVKCERYKDTSCYTFPLRINLKPFCEQSELDDMEYMYDLFSVIIHKGGCYGGHYHVYIKDVDHLGNWQCQEEISDTNVNVKAPQSEEEANDPLVVLKTILLQEEANQIPVDQLGQKLLKKTGISWNKKYRKQHGPLRKFLQLHPQIFLLSTDESTVSLLRNHLTQAPSDLQSCEQILHTLASESPGLNDDTSCPHWFDINDSKVHPIREKDITQQFQGKESAYMLFYRKATLQRPPEARANPRYRVPCHLLKEMDAANILLQMRRAECDSANSTFELHLHLGPHYRFFNGALHPAVSETESVWDLTFDKRKTLGDLRQSIFQLLECWEGDMVLSVAKRVPAGLHVYHTLDGDDLTLCEAEVADGEDIFVWNGVEVGGVQIQTGFDCEPLLLNILHLELSGEGSKCEQLVESPHVFPANAEVGAVFTALGTPAGAILMNSVESADGECWTAVPKEDMKKTFREQGLRNGSLILVQDSDSDNNSLLPKQGRWTNSMNELNWLQVKNFCQSESEEKQVQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The presence of USP domains is critical for the interaction of the designed protein with its intended targets.2. The ideal protein would be one that plays a critical role in regulation of protein stability, protein deubiquitination through cysteine-type deubiquitinase activity, cysteine-type endopeptidase activity within cytosol, nucleus.3. The Nucleophile, Proton acceptor of the protein must be engineered to enable effective catalysis of Thiol-dependent hydrolysis of ester, thioester, amide, peptide   and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-   residue protein attached to proteins as an intracellular targeting   signal).. The designed protein sequence is ",
    "response#1": "VGLKNQGATCYMNSLLQSLYIIHAFRRIVYQIPTDSPQGKDSIAYALQRCFYNLQFMNEPVSTTELTKSFGWDSLDSFMQHDVQEFNRVLQDNLERSMRDTKVENALTNLFVGKMKSYIACVNVNFESARSEDYWDIQLNVKGMKNLEDSFRSYIQVETLEGDNCYFADTYGFQEAKKGVIFESFPPILHLQLKRFEYDFERDMMIKINDRYEFPLEFDAKAFLSPEADQSQNCEYVLYGVLVHSGDLHNGHYYALLKTEKDGPWYKYDDTRVTRATLREVLEENYGGDYIMHPPFRSPVKLKRFMSAYMLLEPYEFVSLEWLQKWLDESTPTKPIDNNACLCSHDKLHPDKISIMKRISEYAANIFYSRYGGGPRLTVKAKPEGHLALDIERFSSFMNKPDSRIMSKPVIVRGIPWRILAICRHQQNNRQVATSRSRNNYNFGFFLQCNNDDLLQKRGMWRCYGQATLEVLNANGPPIQKKIHHSFHNTEVDWGFSNYDQYDTLTSPKDGYVIDDVIRLRCRFTWHIKSWHELDRRAVSPQFAVGSRQFKITYFPQGTLQSAGFTSIFLEYIPSEEEKLSNKYGCCCQFAFVISNPRKPSLSVANSAHCRFSPEIVDWGFTQFAELKKLL",
    "response#2": "MEPILINAQVQMWSAKAGMSKSRNALIETCVGKREVKLILYFSTGKIKTLQLHDNIKSVVLQTYGEDQNYLHLTFKNNDFLFVEKLTTTDARRLKRFLDKTSEPYEFVSLEWLQKWLDESTPTKPIDNNACLCSHDKLHPDKISIMKRISEYAANIFYSRYGGGPRLTVKAVFRLWRMTDSYTVDKPQKMASRLRPSDFIEYSIDTRLDHTLSHDTETIYVEHSQFLQPLNEYLPTRDILFFLKYYDAITDKFTIIGHVTLDSHKRLNLYRMTFCDLLGLPKDTELKYYIEHAPNHVEQILVALKTNGDGNCLMHAASQYMWGVQDTDLVLRKTLFGALKETDTRNFKFRWQNESIKSQEFVETGLRYDTRNWDEEWENLVKMASADTSVSRSALQYNSLEEIHIFVLANILRRPIIIISDKMLRSLESGSSFSPLKVGGIYLPLHWPAHECYRYPIVLGYDSQHFVPLVTGPGGLQNLGNTCFMNSTLQCLAHTPPIVEYFLQDYRSDINAKNPLGMRGELAIAFGELLRKLWSSGQNTVAPRAFKTKLARFAPQFSGYNQHDSQEMLAFLLDGLHEDLNKVKRKPYIEAKDSDGRPDDEVAEEKWKYHKARNDSVIVDVFQGQYKSTLVCPDCGKISITFDPFMYLSLPLPSSRTRSMTVTVFYGDGSHLPMPYTVTVPKDGSCRDLSNALGTACCLDNDESLLLAEVYDHKVFKYYENPRELLNGIKDNEHIVAYRFKQMHKGPGKVKLEILHGEQEKSSDRGPKCFGTPLVTYINKEPLSGTDIATSISGLLSPLRRVHMSCVVNSGNENGHVPDESSRSILSRDTETEDNDRELSLSLLRDYYSFNLQPLESDSVVNPGSVTKVLVKWNEKEHEKYDSSYLNDLPKVHKNVLAKKTMQEGISLFSCLEAFLAEEPLGPDDMWYCPGCKEHRQANK",
    "response#3": "MEPILINAQVQMWSAKAGMSKSRNALIETCVGKREVKLILYFSTGKIKTLQLHDNIKSVVLQTYGEDQNYLHLTFKNNDFLFVEKLTTTDARRLKRFLDKTSLRGIQNLGATCFMSVILQSILHNPLVRNLFFSGFHTSTDCKRPTCMTCAIDDMFSSIYNSKNKSTFYGPTAVLNLMWKLSKSLCGYSQQDGHEFFVYLLDQMHTESGGGTSMPCTCPIHRIFSGSLKNVVTCLDCKKERVAVDPLMDISLDINEPTLQGCLERFVSKEKVQYSCHSCGSKNAIKQLVFDKLPPTICMQLKRFEQNNFAMSTKIDKQVSYPAFLRMRYNFNQDDVDYQLYSVVCHKGTLDTGHYIAYTYYQNQWFLLDDTTIVEVKESEVLNSQAYLLFTGLQNPCNTCYINSIIQCLFGTTLFRDLFLTKKYRLFLNTNKYPKEVQLSRSIYVLFKKMYLNGGRAIIPNRFLKMCKKLRPDLNIPDDQQDTQEFLLIVLARIHEELSNENVVKYYPDLVSYDANALQVNPSKYEKWYERNVITDGLSPIDHIYRGQLENILKCQRCGNSSYSYSTFYVLSLAIPKLSLYSFTSKSRKIKLEDCINLFTGDEELSGDNAWDCPNCRITDSKSKKEEITSQKKKSTIFGFHSRSRSKSPHHHHHHHHSSDDSTKNAKKRNSKKLTTIKSLDFIVLPPILVIHLSRFYYDLTKKNSTVITYPLILNIILKNGKVIRYKLYGTVNHSGNLINGHYTSVVNKEKSHEIGLNRQVWVTFDDDYIQQHRKDRNNFEAGKTEMSSDEVYVLFYER"
  },
  {
    "reference": "MNRLSREQVEQWLATXQPNASVYLVGAGGCGMSGLAHLLLDLGLAVQGSDLRDHAGLXLLRERGAVXHEGHAPEQLRAAQPDLVVYSSAIRMDNPELALAQQLXIPLVRRAVLLAALAHQRRAVCVAGMHGKTTTTALLAYALEQLEAPAGFAVGAGVPQLGRSARMGADASAWFSVEADESDGTLREFHPEQAIVLNIDEEHLDYFANFQAVCDEFMTFAEQTHGRLIFCADDACLLELFGGHARSVSYGFXPAADYXAXVXXSGRFSVXCAXEALGEFSLKLWGEKNISNAVAVVAFLXANGFEADAIREALKGFQGADRRQQELFCDERFRIFDDYGHHPAEIEATLRAIRERCAGRLXVAFQPHRYSRTQHLLSEFAGCFGEADLLWITEVYAASEEPIADIHGQRLVEAIAATGQPAAFTATLDLLRNKVRQAXKPGDVAXFLGAGDITQVAHALAKDLHMTGTTXAQALRELVSTDSEVLENEPLAMRTTLGVGGPAEILVTPANETDLAATMRYAATHEVSVFLLGRGSNLVIREGGIRGIVICLRHESFTQIDVRENVLHCGAGARLKHIANAARDAGLAGLXFLEGIPGCLGGALRMNAGAWGGETFEQVESVRFMTRDGQIEERPGFEMGAVYRSCPVLRDHIALSAVLRGXLESREGVQARMDAFRKQRTENQPHNRSAGCMFKNPGISSAGKLVDECQLKGLQVGGAMVSERHGNFIVNDGTATANDVLTLIEQVRERVKVERGIDLQTEVQVVGE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Cell wall formation..2. The designed protein should contain one or more FAD-binding PCMH-type domains that are essential for its function.3. The protein design should take into account the physiological conditions of the peptidoglycan biosynthesis pathway.4. The protein's active site must be carefully engineered to ensure that the amino acids involved in NADP(+) + UDP-N-acetyl-alpha-D-muramate = H(+) + NADPH + UDP-   N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine are properly positioned for optimal catalysis.5. The protein should be designed to localize specifically to cytoplasm in order to perform its role in regulation of cell shape, peptidoglycan biosynthetic process more effectively. The designed protein sequence is ",
    "response#1": "MNRRMELKGNVGGVVILDDYGHHPTEMQATIEAIRERWPKSRLHVVFQPHRFSRTQQLYKQFAKVLRQPDHVFLMPIYPAGEKPIKGISSQLISKSMKKKNWSFWSFKDSEDDLVNGLKTDDVLLTLGAALRVLREED",
    "response#2": "DVYITPESYSEVQDIIKLANKEDVPFTMLGNGSNLIVKDGGIRGIVMNLQKLASIWREEDKIIAQSGARIIDASRTALAESLAGLEFACGIPGSVGGALYMNAGAYGGEIKDVLESTIVVTKEGDIRTGHFLGDPTPCLHCYETICPPPEGVILARMNEINAARSSTQPIKERTGGSTFANPEGFKSWQLIDKAGLRGYRIGDASISELHCNFMINNGNATAKELEDLGNFVQQKVFEDSGIKLNWEIKRLG",
    "response#3": "ISQEMNRLNHLRQLKQPLEFPSCGSVFKRPPGHFAGQLIMEANLKGHRIGGVEVSEKHTGFMINVADGTAKDYEDLIAYVIETVENHSGVRLEPEVRILVAP"
  },
  {
    "reference": "MGLFTVTKKATTPFEGQKPGTSGLRKKVTVFQQPHYLQNFVQSTFNALPADQVKGATIVVSGDGRYFSKDAVQIITKMAAANGVRRVWVGQNSLMSTPAVSAVIRERIGADGSKATGAFILTASHNPGGPTEDFGIKYNMGNGGPAPESVTDKIFSNTTTISEYLISEDLPDVDISVVGVTSFSGPEGPFDVDVFDSSVNYIKLMKTIFDFEAIKKLLTSPKFTFCYDALHGVAGAYAKHIFVEELGADESSLLNCVPKEDFGGGHPDPNLTYAKELVERMGLGKSSSNVEPPEFGAAADGDADRNMILGKRFFVTPSDSVAIIAANAVQSIPYFASGLKGVARSMPTSAALDVVAKNLNLKFFEVPTGWKFFGNLMDAGMCSICGEESFGTGSDHIREKDGIWAVLAWLSIIAFKNKDNLGGDKLVTVEDIVRQHWATYGRHYYTRYDYENVDAGAAKELMANLVSMQSSLSDVNKLIKEIRSDVSEVVAADEFEYKDPVDGSVSKHQGIRYLFGDGSRLVFRLSGTGSVGATIRVYIEQYEKDSSKTGRDSQEALAPLVDVALKLSKMQEYTGRSAPTVIT",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a fast turnover rate for alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate.2. A protein with phosphoglucomutase activity, magnesium ion binding functions is required to enable carbohydrate metabolic process.3. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.4. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "VPENTQEFAINASNSRFWEPPVKRYVDECLAGKTGPRDKDFNMRWIASMVAEAHRILMRGGVFLYPRDSKDAAKPGRLRLLYEANPIGFIMEQAGGRASTGREPMLGVQPTSLHQRIGLIFGSKNEVERIERYL",
    "response#2": "DGDQIIGLIAAFWKQHDRLRGNTVVTTVMSNMGLEKALAENGLELQRTAVGDRYVVERMREIGANLGGEQSGHMVLSDYATTGDGLIAALQVLAVSVETKRPASEICRFFTGE",
    "response#3": "NGDELLYLMTLDRLAAEPSEDAAPSRVGVPGVVGTLMTNLAVEQALAARGVAFVRAKVGDRYVLEELLARGWQLGGEGSGHLIALDKHTTGDGLVSALQVLQAVVRSGRTLAQLLEA"
  },
  {
    "reference": "MAWFFAPEPVMVTADEALKGGRHPVLENPAPHTVLGTPVTGPWKEGQQRIWIGLGCFWGVEQMYWQMDGVEGTSVGYAGGFTPNPTYREVCSGRTGHTEIVEVVYDPSKISLEQLVARGLEAHDPTQGFRQGNDVGTQYRSAYYTENEEDAARVKAVVDAYGETLKQHGFGEITTEIGVISPSEYFLAEDYHQQYLDKNPDGYCPHHSTGIPCGVEA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein's active site must be carefully engineered to ensure that the amino acids involved in [thioredoxin]-disulfide + H2O + L-methionine = [thioredoxin]-   dithiol + L-methionine (S)-S-oxide are properly positioned for optimal catalysis.2. A protein with L-methionine:thioredoxin-disulfide S-oxidoreductase activity, peptide-methionine (S)-S-oxide reductase activity functions is required to enable protein modification process.3. The protein must exhibit the following characteristics: Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. The designed protein sequence is ",
    "response#1": "MEIAVLGLGCFWGPEIKFSKLEGVIKTEVGYCGGDSKTVTYKEVCTCNTNHAEVVKLDFDPKIISYEKILNFFFEIHDPTTLNSQGPDFGTQYRSEIFYLSDRQKEIAESVIKKVNLKLSGNVVTKSSLLKNYCPAEEYHQRYLEKN",
    "response#2": "MEIAVLGLGCFWGPEIKFSKLEGVIKTEVGYCGGDSKTVTYKEVCTCNTNHAEVVKLDFDPKIISYEKILNFFFEIHDPTTLNSQGPDFGTQYRSEIFYLSDRQKEIAESVIKKVNLKLSGNVVTKSSLLKNYCPAEEYHQRYLEKK",
    "response#3": "MEIAVLGLGCFWGPEIKFSKLEGVIKTEVGYCGGDSKTVTYKEVCTCNTNHAEVVKLDFDPKIISYEKILNFFFEIHDPTTLNSQGPDFGTQYRSEIFYLSDRQKEIAESVIKKVNLKLSGNVVTKSSLLKNYCPAEEYHQRYLEKK"
  },
  {
    "reference": "MTNFRLNVLAYSVMLGLTASVAYAEPTNQPTNQPTNQPTNQPTNQPTNQPTNQPTNQNSNASEQLEQINVSGSTENTDTKAPPKIAETVKTAKTLEREQADNIKDIVTYETGVTVVEAGRLAQSGFAIRGVDENRVAINIDGLRQAETLSTQGFKELFEGYGNFNNTRNGAEIETLKEVNITKGADSIKNGSGSLGGSVIYKTKDARDYLINKDYYVSYKKGYATENNQSFNTLTLAGRYKKFDVLVVTTSRNGHELENYGYKNYNDKIQGKKREKADPYKIEQDSTLLKLSFNPTENHRFTFGADLYEHRSRGQDLSYTLKYQKTDPNLAEVDSRHTNDKTKRRNISFSYENFSQTPFWDTLKITYSDQRIKTRARTDEYCDAGVRHCEGTDNPTGLKVTDGKITRRDGSELQFEKKNNTAHSNGETYDFKKFIDTDKKVIEDKLTLKNPNDTWYDCSIFNCENNAKIKVFEGNYSYGYDGKWKEVDLEIKTLNGKKFAKIKDPTNKIKSILPSSPGYLERLWQERDLDTNTQQLNLDLTKDFKTWHIEHNLQYGGSYNTAMKRMVNRAGNDASDVQWWATPTLGYNFYDQPYTCATAYSWNANLCPRVDPEFSYLLPIKTTGKSVYLFDNLVITDYLSFDLGYRYDNIHYQPKYKRGITPKLPDDIVKGLFIPLPKNSNSKPDEVKKNVQQNIDYIAKQNKKYKAHSYSFVSTIDPTSFLRLQLKYSKGFRTPTSDEMYFTFKHPDFTILPNADLKPEIAKTKEIA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Acts as a receptor for hemoglobin or the hemoglobin/haptoglobin complex of the human host and is required for heme uptake..2. Incorporate a signal peptide in the protein design.3. The designed protein should have transmembrane transporter activity within cell outer membrane to regulate organic substance transport. The designed protein sequence is ",
    "response#1": "WGGHEGSMLFWLFSLSLWLAAFAFFNRKNDRTFSAQSLSLLGLICFGFAVFILFYSNPFGRIFPAPAEGRDLNPMLQDVGLIFHPPLLYVGYVGFAVNFAMSLSALIYNQSARQIARSMRGWVLVSWLFLTIGIVLGAWWAYYELGWGGWWFWDPVENASLMPWLLGLALLHSLMATEKQGVFSYWTTLFSLLAFAFSVLGTFIVRS",
    "response#2": "WGGHEGSMLFWLFSLSLWLAAFAFFNRKNDRTFSAQSLSLLGLICFGFAVFILFYSNPFGRIFPAPAEGRDLNPMLQDVGLIFHPPLLYVGYVGFAVNFAMSLSALIYNQSARQIARSMRGWVLVSWLFLTIGIVLGAWWAYYELGWGGWWFWDPVENASLMPWLLGLALLHSLMATEKQGVFSYWTTLFSLLAFAFSVLGTFIVRG",
    "response#3": "RWVILVIFMFLSGSNGAQWIQYSIIANIISDYYKVSFQAVDWTSMIYMLTYILFFIPAAWLLDKWGLRLSVLLGALGNCVGAWIKLLSTHPDSFWVTFVGQTIVGASQMFTLGIPPRLAAVWFGPDEVSRACALGVFGNQLGIAVGFVLPPLIVSNGSVEHVTYDLNTLFLGSAVLNTSILALVICFFTARPAVPPSLAQVNALEEKTFDNNFWGTLRKLMTSRDFVILFITYGINTGVFYAISTLLSQMVLSVYPNETETVGYVGLLIVVAGMAGSVVGGFILDKFKKFKLTTIMIYLFSFVGMLSFTLTIDLDSMVLVFINAALLGFFMTGYLPIGFEFAAEITYPAAEGTTSGLLNASAQIFGIALTWLMGIVMHGFGTFTSNIIMSSCLVVGTILTCFIREDLETQISAKKLAKQQASDSRDLVRYETGITVVETGRTGASGYAVRGVDENRVGIMVDGLRQAETLSSQGFKELFEGYGNFNNTRNSIEIENVKTATITKGADSLKSGSGALGL"
  },
  {
    "reference": "MRGVWTPDQVRAAEERLGRHVSESTLMYRAAFGVAVAAAELLRDRIGRVAGSRVALLVGAGSNGGDALWAGAFLRRRGVRVDAVLLAPDRAHPAGLAALRAAGGRASAAGAGQVVVSDEAAEVVGRADLVIDGIVGLSARGPLRPAAAALVAAVTAPVLAVDLPSGVDPGTGAVDGPAVSATRTVTFGGLKPGHVLGAGVAHSGRVDVLDIGLAEALGPPELSLLDPADLRRDWPVPGPTDDKYSQGVVGVAAGSAVFPGAAVLVTGAAVHATSGMVRYAGPAADVVRDHWPEAVVTGTVADAGRVQAWSVGPGIGTGESGREVLRHVLGSGLPACVDADAITLLAHDETLWDLRDPGVPLVLSPHAGEFARLAGPVGVDRVASVRRAAARFDAVVLLKGNTTVIAAPDGRVLVNEARGSWAATAGSGDVLTGLIGALLAAGLDPWSAAGNAALLHSTAADLAARGAPIGASALLAAVPAAIRRFRRTEG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a high specificity for AMP, K(+), (6S)-NADPHX binding, with minimal off-target effects.2. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.3. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.4. The protein should have a low Km value for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.5. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.6. The designed protein should have metal ion binding, NADHX epimerase activity, NADPHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding to facilitate nicotinamide nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "MTAIERASEKAGVSTDVLMENAGLAVARQARALVGAAGVQVLVLVGPGNNGADGLVAARHLRRWGAQVTCYVVAGRPDPDAKKDRALEYGVRIIDAAEIGALERLLARSALVIDAVLGTGRARPLAGQVKETMISLAKARSSPTSPKLLALDLPTGLNADTGEVDPFGVPCDLTLALGYPKAGLLNFPGTGHAGELRVLDIGVPPGLPEGPWLRALPEPSDIGAMLPARSVDAHKRSTGSVLVLAGSDGMSGAALLTSRGAARMGAGYVTLVSTSYVDHAKKSLIPEAVSRIVADHSELGPEVLAEFDDDFDRADAVALGPGLGTGPRQQALVETALEAIDVPLVLDADGLNVLAEDAAALRKRTSPTVLTPHPGEMATLLKLSSKDVQADRVGVARRAASDLGCVVVLKGAGTIVAGPEGPAVVNPTGGPELATAGTGDVLTGVVGALLAEGLDPVSAAIAGVFVHGLAGGVAGVSAGGRGTVAWDVAEALPAAVGELYPESDKYRRGVVGVAAGSERYPGAAVLAVAGALRGGAGAVRYVGPPAEAVLARFPEVLIGPGRVQAWVAGSGLGDAPEARERLARALDDGVSTVVDADGLRMLGPAPGRTARGPLVLTPHAGEAAALLGVAREEVEEARLESVRQLARQTGATVLLKGSTTLIASPPGEADAGEEDGGTVLANLNGTGWLATAGTGDVLAGLIGSLLAAGLAPADAAAVGAHLHGLAG",
    "response#2": "MKEIDSYAIHTIGIPSMVLMERAALCVADEMGRAFPAASGRELCRAKTAVFCGTGNNGADGAAIARILHLRGCSVFIVTVGDRSRWTPELRSQIAIARNLGIPVRRFEEPGERTDSFDVAVDALFGVGLSREVEGSFREAIRFFDSVRPMFTVAVDIPSGIHSGTGAVLGAAVRADLTVTFGCEKAGTALYPGRGFAGRICVADIGFPPYDRSKPEVRLSARPRNTHKGLYGDAGVLGGATGMAGAALLAGRAALMLGAGRVFVGLLDPGAPTVDTNHPELMLRNAAALPDHLTALAAGPGLGTDADARQLLSAAIESDCPLLLDADALNIVSGDDKLAQTLSERDGITLLTPHPAEGARLLGITTQEVQHDRLAAALALARKYRSLVVLKGAGSIIATPDGRWFINNTGHPGMATAGMGDVLSGLALSLLAQGWPAEPALIASVHLHGAAADRLAREGVGPVGLTASEVIDGAGRVFVGLLDPGAPTVDTNHPELMLRNAAALPDHLTALAAGPGLGTDADARQLLSAAIESDCPLLLDADALNIVSGDDKLAQTLSERDGITLLTPHPAEGARLLGITTQEVQHDRLAAALALARKYRSLVVLKGAGSIIATPDGRWFINNTGHPGMATAGMGDVLSGLALSLLAQGWPAEPALIASVHLHGAAADRLAREGVGPVGLTASEVIRLSARPRNTHKGLYGDAGVLGGATGMAGAALLAGRAALMLGAGRVFVGLLDPGAPTVDTNHPELMLRNAAALPDHLTALAAGPGLGTDADARQLLSAAIESDCPLLLDADALNIVSGDDKLAQTLSERDGITLLTPHPAEGARLLGITTQEVQHDRLAAALALARKYRSLVVLKGAGSIIATPDGRWFINNTGHPGMATAGMGDVLSGLALSLLAQGWPAEPALIASVHLHGAAADRLAREGVGPVGLTASEVID",
    "response#3": "MKEIDSYAIHTIGIPSMVLMERAALCVADEMGRAFPAASGRELCRAKTAVFCGTGNNGADGAAIARILHLRGCSVFIVTVGDRSRWTPELRSQIAIARNLGIPVRRFEEPGERTDSFDVAVDALFGVGLSREVEGSFREAIRFFDSVRPMFTVAVDIPSGIHSGTGAVLGAAVRADLTVTFGCEKAGTALYPGRGFAGRICVADIGFPPYDRKPACLIGGSEGKMGAVVMSARASLRSGCGLLTAVVPQIGHPILQSTVPEAMLSVDSGKLQLEEFSNNGNFQAFGVGIGMGKGESTVRGFSKWMQDLKSQARIILDADALNILSEHADLLKFLPRQTILTPHPKELQRLIGEWSDDYEKIEMAKTFARAHGVILLIKGAHTAIIDEDGSCYFNSTGNPGMATGGSGDVLTGIICSLLAQGYVPLEAAKLGVFIHGLTADLAKEKFGEISMLPTDLIDFLPTTHKGERGRAFIAGGSPGLTGAVCLAGQAALRVGAGLVTVGVPESLHYLMEVKLTEVMTASLPEAENITLGIGAAGTILERAGRTDALAIGPGLGQGEELPHLLAQLVQEVSVPTVIDADGLNALVGQTDILRLAGAPIILTPHPGELARLCESTVAEVQAHRLPLARELAQEWGVILVLKGARSIVATPEGAYYVNSTGGPALATGGSGDVLTGIIAGLLAQGLTAKEAAIAGVFIHGLAADEAARQVGGEHGVVAGDLIAALRPSLFRPQLTPRARNSHKGSYGDTAILGGAPGMCGAALLAARAALWLGTGRVYAGLLDPRAPTVDVSNPELMLRPADELPEQVSALAIGPGLGRSDAAAALLARSVCLDRPLVLDADALNLLAADPALQRVLAARAAPTLLTPHPAEAGRLLASDTAAVQADRVAAALELSARLRAEVVLKGCGSVIACSDGRWYINGTGHPGMASAGMGDVLTGLLVALLAQGWPAERALIGAVHLHGAAADRLSREGIGPVGLTASEVIGTARGLLNG"
  },
  {
    "reference": "METGTLTIAVEDKKPGEPGPEKPDGGKGGAGEGAKAREGKQSPAPERKKPTKLSEDKRFKLFTGTANPKLAEAIGKHIGVPVGEAKLQRFADGEVYFQLLENVRGVDVFVVQPTCYPVDQHLVELLVMIDALKRASAARITVVVPYYGYARQDRKDRPRVAISAKLIADLLTTAGANRALFVDLHAAQIQGFFNIPVDHLFASPVLVSYFREMNLPNLTVVSPDAGGVERARFFAQKMGAPLAIVDKRRTDMNVAEVMHVVGDVHGKTCLIIDDIIDTAGTLVKTVDALYASGAPAVYACASHAVLSGPAIDRIANSRLEQLVVTDSIPLREAAQKLTQPANDPAQKLPKLKVLTIAGLLGAAIESIHMETSVSTLFS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.2. The designed protein should interact with enzymes and substrates within the 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, Metabolic intermediate biosynthesis pathway to enhance or regulate its activity.3. The designed protein should have a high specificity for D-ribose 5-phosphate, ATP binding, with minimal off-target effects.4. The designed protein should have kinase activity within cytoplasm to regulate phosphorylation, nucleotide biosynthetic process.5. For general function, the protein need meet that Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P). The designed protein sequence is ",
    "response#1": "MIFSGTANEELSSEIAKYLEMPLSDATINRFSDGEINVQIAQSVRGKDVFIIQPTCAPANDNLMELLIMTDALKRSSAKSITAVVPYYGYARQDRKAAPRVPISAKLVANLMETAGKILEKHFFDKGLADAIVVAPDNGGLGRARKLASHLDAPIGFIDKRRMHPGEPEVVNIVGDIKGKNVIIIVDIIDTATTISLAANVLVENGAKAVYACCTHAVLSGQAVQRIEESAITELVVTNVRFGNDIAVPVLHHYIEKLELDPDELAIVSPDEGRIKLALKLQRRLGGTLAVIDKRRSSATETDQVSIIGGPVDGKIAIMFDDMISTGGSVVGAARVLHASGARDIRVCVTHGLFCNNALERMRQSPLVQIVATDALPLLVDWLVQRYGDEHLVVASPDAGGVKLAQKFQSRLRNASIAFLAKTRTAHNIAKTLAVVGEVEGHTCVLVDDMIDTAGTIVGAAEALIARGAKAVVVVATHPVLSGPACERLQDSPIETVVVTNTCPLPPEKAFSKQPRLRVLEVANLIGERIQRVV",
    "response#2": "MQIFSGRGYPELAHEVVDHLGMRLGDVDIHEFASGEIYCRYRENVRGSDVFVVQTHCHPLHDNIWEQLELIDAAKRASAKRIIAVVPYYGYARQDRKARSREPITARLLADMLCVARRAGVRSLDLHSGQIQGFFDQPLDALAPVMTRDIKEHYDTTNVMVVSPDVGGVVRARALAKRIEAPLSIVDKRRDKPGESEVMNIIGDVSGYDCILVDDIVDSGGTLCNAADALLAAGAKSVTAYITHGVLSGGAVARVSASKLKELVITDTLPLDERAAGFEAIHLLSNYFRHKELVDPVVVTDLGFAKRGRAFAELLDAPLAVIEKRRVGNLDRAELLNVIGEVRGRRAVIVDDEIDTAGTLLEIVRALQREGVTEIYACATHGILSGPAVERLQDAGIEQIVLTDTIPVPGEIAREFPSLTVLTVGELIGDRIHAVQTEESMF",
    "response#3": "MKIVACNSNRPLAEAVAAALNLPLTKASVRRFADMEVFVEIHENVRGEDVFVIQSTSYPANDNLMELLITLDALRRGSARRVTAVIPYFGYARQDRKSGPRTPISAKLVANLITEADLKSGIDTITRWFEGASPVMVSYFRELNLPNLTVVSPDAGGVERARFFATKMGAPLAIVDKRRTDINVTEVMHVIGDVKGRTCLIIDDIVDTAGTLVKTVDALLEQGATKVFACASHPVLSGPAVERIANSRLEELVVTDAAPVLIGHLXDRGIDNLTVVSPDVGGLKMSDAYAQALDXPLAIVGKRRVSATEVEAINVIGEVEGRDVMLVDDMTETAGTLCAAAELLKENGAGKIYAGVSHGVLGEIGLKRLKDSCIEELITTDEKHIRNAVPVLHHYIEKLELDPDELAIVSPDEGRIKLALKLQRRLGGTLAVIDKRRSSATETDQVSIIGGPVDGKIAIMFDDMISTGGSVVGAARVLHASGARDIRVCVTHGLFCNNALERMRQSPLVQIVATDATVGVPRAVELLKNGATVGEVVHRDLL"
  },
  {
    "reference": "MRVRETQMNWPNLWRWGTLILGLVIICSASDNLWVTVYYGVPVWRDADTTLFCASDAKAHDTEVHNVWATYACVPTDPNPQEIHLENVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLTCTTANLNNTTEAHNNSTGNMIGNLTDEVRNCSFNMTTEIRDKKQKAYALFYKLDIVPIHGDDITYRLINCNTSVIKQACPKVSFDPIPIHYCTPAGYAILKCNDKKFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENITDNTKNIIVHLNQTVEINCTRPNNNTITSLTIGPGQVFYRTGDIKGDIRKAYCEINATKWYKVLKQVTEKLKEHVNKTINFKPPSGGDLEITTHHFNCRGEFFYCNTTQLFNNTHRVNDTGINGTIILPCRIKQIINMWQGVGQAMYAPPISGRINCVSNITGILLTSDGDENATFRPGGGDIKDNWRSELYKYKVVQIEPLGIAPTRAKRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQTFLGLWGCSGKIICTTNVPWNSTWSNKSFKEIWDNMTWIEWEREISNYTNQIYEILTESQNQQDRNEKELLELDKWASLWNWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTPTHHQREPDRPERIEEGGGEQGRDRSVRLVSGFLALVWDDLRSLCLFSYHRLRDFGLIAAR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain2. The protein design must feature the Di-leucine internalization motif motif. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPXWEEANPILFCASDAKTYSSEKHNVWATHACVPTDPSPQEIAIKNVTENFNMWKNNMVEQMHEDIISLWDESLKPCVKLTPLCVTLSCTDVNSTDVNSTGNITRNIKEEMTNCSFNTTTEIRDKRKKEFALFYKLDVVPINSSSNSYMLINCNVSSIKQACPKVNFDPIPIHYCAPAGFAILKCRDKEFNGTGPCNDVSTVQCTHGIKPVVSTQLLLBGSLAEGKIRISSENITDNTKVILVQLNETIEINCTRPSNNTRKSIPFGPGQAFFATEDIVGDIRQAHCNVNKLKWKEMLQKVQERLREXYNRSIFFNSSSGGDLEITTHSFNCRGEFFYCNTSGLFYNMTYINTSNISENDTIILPCKIKQIVRMWQRVGQAMYAPPIAGNITCNSTITGLILTRDGGDQNAENETFRPAGGDMRDNWRSELYKYKTVKIKSLGVAPTRARRRVVGREKR",
    "response#2": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLALERYLKDQTFLGLWGCSGKIICTTNVPWNSSWSNKSYEEIWNNMTWTEWEREISNYTNQIYEILTESQNQQDRNEKELLELDKWASLWNWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSLQTPY",
    "response#3": "LWVTVYYGVPVWKEATTTLFCASDAKAYDAEVHNVWATHACVPTDPNPQEVVLGNVTETFDMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVVLNCTNPTNTSSRTNSTC"
  },
  {
    "reference": "MKLAELMQPWVRGDIPECEILGLHNDSRQIKPGFLFFAYPGLATDGRLFIPQAVHAGAAALVYDPENWSPADHISSNFPAIPLPGLAAKLGAIASRFYGEPTKKLAITGVTGTNGKTTIAYQLAQAHNLLGEHAAYVGTIGQGEVTALKPLINTTPDALCLQELMHQYEQAAIKQVCMEVSSHALCQGRVDGIDFQQAIFTNLSHEHLDYHLNMQAYAEAKALLFAMPMLKWAIINQDDPYSQLMRQQSKAKQLISYGVRENAEVKALSWDVSLKGTELELISPWGQHELIINALGFFNIYNALAVFTSLLAYGYPVDKVVAVMAKLQAAPGRMEIVSKEPYAIVDYAHSPDALENVLATLQNVKKGRIIAVFGCGGDRDKTKRPIMGRIASQYADITIITSDNPRTEDPLAIIESIESGIDSKTNLYKIPDREQAIAKAINLAEKEDIILVAGKGHENYQQIGNVRHAFSDQAIIRRLNKQPAQI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must be able to regulate cell wall organization, peptidoglycan biosynthetic process.2. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.3. A protein should be able to manipulate the activity of enzymes in the peptidoglycan biosynthesis pathway, either by activating or inhibiting their function.4. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.5. The protein should be able to bind meso-2,6-diaminoheptanedioate ligand in a variety of conditions6. The designed protein have Mur ligase N-terminal catalytic domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MKLDSREVRPGDIFCAIPGTKSDGRAFIRVAAARGAAGVIYEADGTPQGEHYPVASLAVSHLASRLGEIAALFYGQPSRKLTMVAVTGTNGKTTSTFWLGQLLTKLGHKVGLIGTIETIIGDKVIPSSRTTPESLDLQKLFYEMVDAGCTYCVMEVSSQGLMLHRTAGFTFEYGIFTNLEPDHIGPGEHASFEDYLQVKGLLFRQCRLGIANTDDEHFWRLLEGHTCEVETFGFSPEADLRAVDLQLQEPGTLGVRYRTAGLDFPVRTRLVGRFNVYNVLAAISTCLLEGIPLEEIKAGLESVTVVPGRLEKIDCGQKFTVIVDFAHTPAALENVLMMIKEFRPRKLITVFGCGGNRDRTKRPVMGEIAVSLSDYVIITSDNPREEDPEKIVLDIEVGIKRLNKDNYEVIIDREKAIERALSIANKNDFVLIAGKGHEDYQEIKGIRHPMSDREVVREFLAERGY",
    "response#2": "MKLDSREVRPGDIFCAIPGTKSDGRAFIRVAAARGAAGVIYEADGTPQGEHYPVASLAVSHLASRLGEIAALFYGYPSRQLRLVGITGTNGKTSSTLLLKQVLETVLGHQTGLIGTIEINYGSKILPASNTTPESYDLQRLFAQMVDAGCSYCVMEVSSHALAQHRVDGCRFEVAVFTNLTQDHLDFHGSMEHYFEAKASLFSQLGNSVQSKAAVINMDDSYGQRMLEATQAEVWTYSLDKNADFRASDMQLSMRGTRFSVLFHGESFDISLPLIGRHNIYNALAAIACALHLGVELETIKASLSQVKGVSGRFELVVDADPFSVIVDYAHTPDALRFVLSSVKELTSGRLIAVFGCGGDRDRGKRPLMGEIAASIADYTIVTSDNPRSEDPVAIIDEILDGIDRDRTPVGVEPDRRAAIEQALSAARAGDSVVIAGKGHEDYQIIGTEKIHFDDREEARKAIQDRLV",
    "response#3": "MKKITGTSGKTTSSYLIEAGLAAAGRSTGLVGTIETRVVGRRVPSALTTPEAPQLHALFAAMHERGIDTVVMEVSSHALALGRVDGIRFSIGAFTNLSQDHLDFHKDFDDYFATKGRLFAADSTVRADRAVICVDDEWGVRMSDIARRAHPDQADAVATIGTCPDVQRERDEPPDWIAGPSHTTKSGRQVFALTGPDGATREVSLRLPGQYNVANAALAAAVALLAGFDELAILRNLERFKGVPGRVQRVDRGQDFLAVVDYAHKPAALEAVIATLRGQASGRIVVVVGAGGDRDTGKRALMGEAGARGADLLVITDDNPRTEDPATIRAALAAGARAVPSAERGEVREIGDRAEAIGVAVRWARPGDVVLVAGKGHETYQVIGDRVLPFDDRQLAREALLQRVA"
  },
  {
    "reference": "MSELAASLLSRVILPRPGEPLDVRKLYLEESTTNARRAHAPTRTSLQIGAESEVSFATYFNAFPASYWRRWTTCKSVVLRVQVTGAGRVDVYRTKATGARIFVEGHDFTGTEDQPAAVETEVVLQPFEDGGWVWFDITTDTAVTLHSGGWYATSPAPGTANIAVGIPTFNRPADCVNALRELTADPLVDQVIGAVIVPDQGERKVRDHPDFPAAAARLGSRLSIHDQPNLGGSGGYSRVMYEALKNTDCQQILFMDDDIRLEPDSILRVLAMHRFAKAPMLVGGQMLNLQEPSHLHIMGEVVDRSIFMWTAAPHAEYDHDFAEYPLNDNNSRSKLLHRRIDVDYNGWWTCMIPRQVAEELGQPLPLFIKWDDADYGLRAAEHGYPTVTLPGAAIWHMAWSDKDDAIDWQAYFHLRNRLVVAAMHWDGPKAQVIGLVRSHLKATLKHLACLEYSTVAIQNKAIDDFLAGPEHIFSILESALPQVHRIRKSYPDAVVLPAASELPPPLHKNKAMKPPVNPLVIGYRLARGIMHNLTAANPQHHRRPEFNVPTQDARWFLLCTVDGATVTTADGCGVVYRQRDRAKMFALLWQSLRRQRQLLKRFEEMRRIYRDALPTLSSKQKWETALLPAANQEPEHG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. The protein should have a fast turnover rate for beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-   alpha-L-rhamnosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-   trans,octa-cis-decaprenol + 28 UDP-alpha-D-galactofuranose = [beta-D-   galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-   galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-   rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-   trans,octa-cis-decaprenol + 28 H(+) + 28 UDP.3. The designed protein should have lipopolysaccharide-1,6-galactosyltransferase activity, glycosyltransferase activity, transferase activity, UDP-galactosyltransferase activity within cytosol, plasma membrane to regulate UDP-D-galactose metabolic process, cell wall polysaccharide biosynthetic process, capsule polysaccharide biosynthetic process, cell wall organization. The designed protein sequence is ",
    "response#1": "MLDRYWTGATRRISPEAPVPVVHVDRVEDRPGGAANVALNLAALGVRTLLIGVTGADEAADILAERLADQSIETRLHRRADLPTITKLRVLSHHQQLIRLDFEQSLTPLGEDPLPSMIEAALADADLLLLSDYAKGTLSDPQLLIRLARELDKPVLVDPKGRDFVRYRGATLLTPNRGELEEVVGVCGDDAVLVERARNLRDELDLDAMLVTLGERGMLLVRADAEALHLPTQAREVFDVTGAGDTVIATLAAALAAGVGIEESCALANCAAGLAVGKLGTATVGARELERLEAAVAGPAPAAGPRAAVSAAAAPSAVAAPLAAGGGEPDVSPVPTAARHATPAAAVASPAAGGRARRRPAVVFTNGCFDLVHTGHIELLTSAAAEGDRLIVGLNSDASVKRLKGPTRPIQTEMDRARIVGALRAVAGAELVVLRPEDPADLVRALEETAAGGAAPADGTAAAGVTSGGATSGGAAADGPDAAGGADRVLVLGDLMLDRYWTGSASRISPEAPVPVVRVADTEERPGGAANVALNMARLGVQASLAGMVGEDEAGASLRRLMDAAGIRCRFHVCPGVPTITKLRVISQHQQLLRLDFEQRLNGADAGALLEDTRAAVSSGLSALVLSDYGKGTLERAPELIAAARTADVPVLVDPKGLDFERYRGATLITPNLAEFEAVAGHCAHDDQLAERGERLRVELDLDAMLITLSERGMLLVRAGHDPLHLPTRAREVFDVTGAGDTVIAVVAAALAAGRDLAEAAALANLAAGIVVGKLGTGFVTPAEL",
    "response#2": "MLDRYWTGATRRISPEAPVPVVHVDRVEDRPGGAANVALNLAALGVRTLLIGVTGADEAADILAERLADQSIETRLHRRADLPTITKLRVLSHHQQLIRLDFEQSLTPLGEDPLPSMIEAALADADLLLLSDYAKGTLSDPQLLIRLARELDKPVLVDPKGRDFVRYRGATLLTPNRGELEEVVGVCGDDAVLVERARNLRDELDLDAMLVTLGERGMLLVRADAEALHLPTQAREVFDVTGAGDTVIATLAAALAAGVGIEESCALANCAAGLAVGKLGTATVGARELERLISPEAPVPVVHVDRVEDRPGGAANVALNLAALGVRTLLIGVTGADEAADILAERLADQSIETRLHRRADLPTITKLRVLSHHQQLIRLDFEQSLTPLGEDPLPSMIEAALADADLLLLSDYAKGTLSDPQLLIRLARELDKPVLVDPKGRDFVRYRGATLLTPNRGELEEVVGVCGDDAVLVERARNLRDELDLDAMLVTLGERGMLLVRADAEALHLPTQAREVFDVTGAGDTVIATLAAALAAGVGIEESCALANCAAGLAVGKLGTALARAPSAGPGPGAVAVIGDIMVDRYLTGRIERISPEAPVPVLVHNHERTVAGGAANVAMNIASLGCAVHLVGAIGSDADGEDIRTLLSDAGISPEWLVVDPGRPTISKTRVVSGRQQLVRIDRELTGRLASEVEDRIIRAAEAAISKADIVVLSDYAKGVFGDRVLLEVIGAAKSAGKPVLVDPKRRTFEVYRGADVIKPNAGELSAATGLPCATDQDLEAAALELTKQFAGTLVVTRGEAGLSLFSPGQPVRHITTVAQEVADVSGAGDTALAALAVAVAEGLSIDDAAVISNFAAGIAVGKLGTA",
    "response#3": "MLDRYWTGATRRISPEAPVPVVHVDRVEDRPGGAANVALNLAALGVRTLLIGVTGADEAADILAERLADQSIETRLHRRADLPTITKLRVLSHHQQLIRLDFEQSLTPLGEDPLPSMIEAALADADLLLLSDYAKGTLSDPQLLIRLARELDKPVLVDPKGRDFVRYRGATLLTPNRGELEEVVGVCGDDAVLVERARNLRDELDLDAMLVTLGERGMLLVRADAEALHLPTQAREVFDVTGAGDTVIATLAAALAAGVGIEESCALANCAAGLAVGKLGTATVGARELERISPEAPVPVVHVDRVEDRPGGAANVALNLAALGVRTLLIGVTGADEAADILAERLADQSIETRLHRRADLPTITKLRVLSHHQQLIRLDFEQSLTPLGEDPLPSMIEAALADADLLLLSDYAKGTLSDPQLLIRLARELDKPVLVDPKGRDFVRYRGATLLTPNRGELEEVVGVCGDDAVLVERARNLRDELDLDAMLVTLGERGMLLVRADAEALHLPTQAREVFDVTGAGDTVIATLAAALAAGVGIEESCALANCAAGLAVGKLGTALTASATAVPAEPDLPALDALKEDRLEAFGQLMNASHRSLRVDYEVTGKELDTLVEAAWAQPGVLGARMTGAGFGGCAIAIVEDDTVETFMTAVGRRLGDT"
  },
  {
    "reference": "MSQAIMKQAEERMEKAHLSLKKELATLRAGRANVSILDPVQVDYYGSPTPLNQVANVNTPEARLILITPWDKSMVTEIEKAIQRADLGLAPSSDGTVIRLAIPPLTEDRRKELVKLVKKYTEEGKVALRNIRRDTNEQLKKQEKDGVLTEDDLRGYTEDVQTLTDKFVKVLDQTAADKEQEIMEV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should be able to enhance the efficiency of translational termination.2. For general function, the protein need meet that May increase the efficiency of translation by recycling ribosomes from one round of translation to another. The designed protein sequence is ",
    "response#1": "QRNFNTIRTGRANTSLLDRLTVEYYGADTPLKSLATISTPDSQTLAIQPFDLGSLGLIEKAISISDLGFTPNNDGKIIRINIPPLTEERRKQFCKLASKYAEEAKISLRNIRRDAIEKIKGSEKDGEFSEDQSRDQQDSIQKITNKYINEIEKNLSGKEEEIL",
    "response#2": "KEDFASIRTGRPSPATFSRITVEYYGAMTPVNQLASFQVPEPRMVIISPYDKAAMPAIEKAIRESDLGVNPSNDGNIIRVVFPELSEERRKEYVKVARNKAEDGRTSVRNVRRQAKDAIDKAVKAGEIGEDEGHRAQKELDALTQKYVGEIDELLKHKESELS",
    "response#3": "HDDMKGVRTGRANASLLDGIVVNIYGGHQKLNQVAGVSAIDNKTLSVKVWDATAIGEVKNAIINANLNLNPVVEGNTIRIVLPDLTQETREKLVKLLHQFSENARVAIRNIRRDVMEEIEEMKKNKEISEDDFHVAKKEIQNITDDNVKKVDDDLSIKEKDIS"
  },
  {
    "reference": "MKIVVATHNEGKLVEMRNILQDELGDTAADIELVSAGSLGLPDPVETGVSFEENALIKAQDVALRTGLPAVADDSGLVVDVLGAAPGILSARWAGAHGHDRANMALLLAQIEDIPDGRRQAHFSCAAALVVPKAEEGSADGGTVYAVASQTVEEGRMTGFIIREMRGDQGFGYDPIFVPDDQPARAKDADESLTSAELTPAEKNAISHRGKAVKALAPAIKNLIVGA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..2. The substrate, Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.3. We need a protein that can perform dITP + H2O = dIMP + diphosphate + H(+) with high efficiency.4. The designed protein should be optimized for ribonucleoside triphosphate phosphatase activity, nucleotide binding, XTP diphosphatase activity, ITP diphosphatase activity, metal ion binding activity in order to improve nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "MSILSGKVVVATHNQGKIKELREMLAPYQVELVSAGSLNLPEPAETGTTFVENALIKARHAAKASGLPALADDSGLVVDALGGAPGIFSARWAGPDKDFALAMEKVWSELEARSVPAERRAAFVSVLCLAWPDGHCETFRGEVDGTLTWPPRGSNGFGYDPVFVPDGHDLTFAEMASDQKHAMSHRADAFRKLVAECFGT",
    "response#2": "MLRLLVATRNRHKVVELRRMLADLPVELVGVDQFPDVPEAPETGATFEENARLKAETVHRFASLPVLADDSGLCVDALGGEPGVRSARYAGEPRSDERNNDLLLAQLRDVPDERRTAHFVCVIALARPEGEPVPIATEGRWPGRILREPRGTNGFGYDPVLFDPALGRTAAQLTADEKNAVSHRGQALRALAPMLRQLWTSAR",
    "response#3": "MSIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTSSDELPLRLKLIYAGVTEIITQFNPDCFAIEQVFMAKNADSALKLGQARGAAIVAAVNRDLPVFEYAARQIKQTVV"
  },
  {
    "reference": "MASLRAEHAVGGSWLPATRSGRPAALRLLLLLGAVLKPQESLAQLLPTPDSSKSEGKTVLETYGTNVLQCWHDYKVQMDSIKKDWCDWALISRPYSFLRDCLEKGAEEFGLGFPNPWAEEIIFETHNIHFANCSLVQPTFSDPPEDVLLAMIIAPICLIPFLVTLVVWRSKDSEAQT",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein localizes to the amylin receptor complex 2. The designed protein sequence is ",
    "response#1": "KDDEADSLLNQMFVVRFLGSMAVKSDQTVEVIYEAMRQVLAARAIHNIFRMAESHLLVTSNDLKLIDPQTQVTRANFDLANVTQFAAHQENKRLIGFVVCVSESPGEDSMSTYVFESNTEGEKICYAISLGKEC",
    "response#2": "LYYLAIVGHSLSIFTLVISLGIFVFFRSLGCQRVTLHKNMFLTYILNSMIIIIHLVEVVPNGELVRRDPVSCKILHFFHQYMMACNYFWMLCEGIYLHTLIVVAVFTEKQRLRWYYLLGWGFPLVPTTIHAITRAVYFNDNCWLSVETHLLYIIHGPVMAALVVNFFFLLNIVRVLVTKMRETHEAESHMYLKAVKATMILVPLLGIQFVVFPWRPSNKMLGKIYDYVMHSLIHFQGFFVATIYCFCNH",
    "response#3": "ELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESTLKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIKDLLNHAFN"
  },
  {
    "reference": "MQRIMLRAKIHRAKVTQADLHYEGSCGIDADLLEAADIKVGEKIELYNINNGERFSTYAIQGARGSGEISLNGAAARCAHLGDLMIICTYGPMTDEEIATYTPKIVFVDEDNHFAGMKK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be designed to have high stability and activity towards H(+) + L-aspartate = beta-alanine + CO2. The designed protein sequence is ",
    "response#1": "LQPLKEQFGLKRVNYTTYQAVSGSGSGGLRDLEEGARGKAPTTYPHPIHDNVLPHIDVFLENGYTKEEQKMIDETRKILELPDLAVSATCVRVPVSNSHSVHMNVTLENDATEEEVRDAFRNIPHITLVDNPENNEYPTPLESTGRSEVFVGRVRRDDSLENTFHVWCTADNILS",
    "response#2": "LQPLKEQFGLKRVNYTTYQAVSGSGSGGLRDLEEGARGKAPTTYPHPIHDNVLPHIDVFLENGYTKEEQKMIDETRKILELPDLAVSATCVRVPVSNSHSVHMNVTLENDATEEEVRDAFRNIPHITLVDNPENNEYPTPLESTGRSEVFVGRVRRDDSLENTFHVWCTADNILS",
    "response#3": "LQPLKEQFGLKRVNYTTYQAVSGSGSGGLRDLEEGARGKAPTTYPHPIHDNVLPHIDVFLENGYTKEEQKMIDETRKILELPDLAVSATCVRVPVSNSHSVHMNVTLENDATEEEVRDAFRNIPHITLVDNPENNEYPTPLESTGRSEVFVGRVRRDDSLENTFHVWCTADNILY"
  },
  {
    "reference": "MSLVVQEQGSFQHILRLLNTNVDGNIKIVYALTTIKGVGRRYSNLVCKKADVDLHKRAGELTQEELERIVQIMQNPTHYKIPAWFLNRQNDITDGKDYHTLANNVESKLRDDLERLKKIRAHRGIRHFWGLRVRGQHTKTTGRRRA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The ideal protein would be one that plays a critical role in cytoplasmic translation, endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA), rRNA export from nucleus through rRNA binding, structural constituent of ribosome within mitochondrion, cytoplasm, cytosol, small ribosomal subunit, cytosolic small ribosomal subunit.2. For general function, the protein need meet that The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.; The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain; Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell; The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules The designed protein sequence is ",
    "response#1": "MEQIAAHVTVRGEKAAEILEKGLKVREFELRARNFSTQGSFGFGIQEHIDLGIKYDPSIGIYGMDFFVVLSRPGFRVAHKKK",
    "response#2": "MQIAAHVTVRGEKAAEILEKGLKVREFELRARNFSTQGSFGFGIQEHIDLGIKYDPSIGIYGMDFFVVLSRPGFRVAHKKPPRKPKQDALLAKLNANDANANANADADAGADADAGGGDGAAEPQPQPEPQPQPQTQPQPQPQPSPQPQPKPQPKPQPKPQPKPQPARPQPGPPQQAPQGQPQGQGQGQGQPQGQGQGRPPQGGQQGGRPPQGGRPGGQGQGGRPPQGGRGGGQGGGGRPPQGGRGGGQGGGGRPPQGGRPQGGQGGGRPQGGQGGRPQGGRPQGGRPQGGRPQGGRPQGGRPQGGRPQGGRPQGGRPQGGRPQR",
    "response#3": "MLQIAAHVTVRGEKAAEILEKGLKVREFELRARNFSTQGSFGFGIQEHIDLGIKYDPSIGIYGMDFFVVLSRPGFRVAHKKAITRGEAKWS"
  },
  {
    "reference": "MQFGVPLLVLCLALGSTEATISLKLVSRLYLPFDKLPVGGGAGGAGGAGGAGGGGGGGGGAGGRGGGGGGRGQTGGMYALNNGVAFKSAYDMDKQLAYVGGGQFVQIVDFSDVVQPKVVKQIATEGPVADIAECGDLVAFTQPGKPHFTDVGSLKIYEKYNPATMMMKELCSVEVGSQPIAVRFAQGCSLIIVANEGVMGENNYTKKYVNPEGTITTVRLAGSVSNGPSQQIVNTTFGAGGPMMTTPMAGFPRGTTWSPNAGAGGQGGQGQYPGQGGQGGQGGQGGQGQYPGQGGQGGQGGQGGQGGQGGYPGQGGQGGPGYYPGQGGQGGQGGQGGWGQGGGQGGQGGGNNPQYPMIPTTPLPGTICNGTSTMSYVVSQINFHKFNAPAEVQRLKALHVRQPYTGQLGDPGPHTFSRGLEPRHITLDQQEQIAYISLQENNAIAVVDLNNNTVIDILPMGVKNWKGLKIDASSADRGILFQTYDQLNSFPMPDAIETYYDAMGDLYVVTANEGAKPMMAQCSLEVCPGGPGEFEEVEIGEEFIVEELLPQPVIDSPLGQAMAEETQLGSSLFSMVDGINPAEPEFFNEVFMFGGRGISAYKVDPITMNMTLAWDSGDVIEKEIAKFFPKIFNGAAFSRPPQRVKPFMTKDSRSSGRGPECESLAVGDVQGRKLIFVGIDGVSALAIFSVAPGNSTPVYESLFKDGHIDASYNALYKNRKTGRVSGTVSMYEVIDVPYWMLLKTLGTNIEGSSAISMTSSAFSIFFAF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Not known; Could be involved in mesenchyme cell migration, adhesion, fusion, or spicule formation.2. Incorporate a signal peptide in the protein design.3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "VLQKPAVFLNMTTDWPALHWTVEHLSACLTKRIRFRVGKRSEDMAPLFETECSYVEATIKEFLSWTANDGEPLVGPFLDYHCKEFWAYADYKYIAQLFQDKPAMFQDVVWSDFGFPGRDGRDSTLWIGTQCANTPCHLDSYGCNLVFQIQGRKRWHLFPPDDTACLYPTRVPYEESSVFSHVNVIRPDLKKFPAYGRARLYTVTLQPGQVLFVPRHWWHYVESVDPVTVSVNSWIEMDML",
    "response#2": "VLQKPAVFLNMTTDWPALHWTVEHLSACLTKRIRFRVGKRSEDMAPLFETECSYVEATIKEFLSWTANDGEPLVGPFLDYHCKEFWAYADYKYIAQLFQDKPAMFQDVVWSDFGFPGRDGRDSTLWIGTQCANTPCHLDSYGCNLVFQIQGRKRWHLFPPDDTACLYPTRVPYEESSVFSHVNVIRPDLKKFPAYGRARLYTVTLQPGQVLFVPRHWWHYVESVDPVTVSVNSWIEMDMV",
    "response#3": "ERRDLAVDFIFCLVLVEVLKQIPVHPVPDPLVHEVLNLAFKHFKHKEGYSGTNTGNVHIIADLYAEVIGVLAQSKFQAVRKKFVTELKELRQKEQSPHVVQSVISLIMGMKFFRVKMYPVEDFEASFQFMQECAQYFLEVKDKDIKHALAGLFVEILIPVAAAVKNEVNVPCLKNFVEMLYQTTFELSSRKKHSLALYPLITCLLCVSQKQFFLNNWHIFLQNCLSHLKNKDPKMSRVALESLYRLLWVYVIRIKCESNTVTQSRLMSIVSALFPKGSRSVVPRDTPLNIFVKIIQFIAQERLDFAMKEIIFDLLSVGKSTKTFTINPERMNIGLRVFLVIADSLQQKDGEPPMPTTGVILPSGNTLRVKKIFLNKTLTDEEAKVIGMSVYYPQVRKALDSILRHLDKEVGRPMCMTSVQMSNKEPEDMITGERKPKIDLFRTCIAAIPRLIPDGMSRTDLIELLARLTIHMDEELRALAFNTLQALMLDFPDWREDVLSGFVYFIVREVTDVHPTLLDNAVKMLVQLINQWR"
  },
  {
    "reference": "MVNFTVDEIRALMDRKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGSKAGETRFTDTRKDEQERCITIKSTAISLFFELEKKDLEFVKGENQFETVEVDGKKEKYNGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLFMNKMDRALLELQLGAEELFQTFQRIVENINVIIATYGDDDGPMGPIMVDPSIGNVGFGSGLHGWAFTLKQFAEMYAGKFGVQVDKLMKNLWGDRFFDLKTKKWSSTQTDESKRGFCQFVLDPIFMVFDAVMNIKKDKTAALVEKLGIKLANDEKDLEGKPLMKVFMRKWLPAGDTMLQMIAFHLPSPVTAQKYRMEMLYEGPHDDEAAVAIKTCDPNGPLMMYISKMVPTSDKGRFYAFGRVFSGKVATGMKARIQGPNYVPGKKEDLYEKTIQRTILMMGRFIEPIEDIPSGNIAGLVGVDQYLVKGGTITTYKDAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIFEESGEHIIAGAGELHLEICLKDLEEDHACIPLKKSDPVVSYRETVQSESNQICLSKSPNKHNRLHCTAQPMPDGLADDIEGGTVNARDEFKARAKILAEKYEYDVTEARKIWCFGPDGTGPNLLMDVTKGVQYLNEIKDSVVAGFQWATREGVLSDENMRGVRFNVHDVTLHADAIHRGGGQIIPTARRVFYASVLTAEPRLLEPVYLVEIQCPEAAVGGIYGVLNRRRGHVFEES",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be expressed specifically in the cytoplasm2. For general function, the protein need meet that Catalyzes the GTP-dependent ribosomal translocation step during translation elongation The designed protein sequence is ",
    "response#1": "IRNFSIIAHIDHGKSTLADRLLETTQTVAQRDMEAQLLDNMDLERERGITIKLQAARVLYQARDGEMYCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQGIEAQTLANVYLALENDLEIIPILNKIDLPAADPERVAEEIEATIGLDCSGIVHASAKTGIGIDDILQKIRNFSIIAHIDHGKSTLADRLLETTQTVAQRDMEAQLLDNMDLERERGITIKLQAARVLYQARDGEMYCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQGIEAQTLANVYLALENDLEIIPILNKIDLPAADPERVAEEIEATIGLDCSGIVHASAKTGIGIDDILERIVQMVPPPQ",
    "response#2": "NFSIIAHIDHGKSTLADRILEMTDTIAKRDMQAQVLDDMELERERGITIKLNAVELHYHAKDGETYIFHLIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQTLANVYLAVDDDLEIVPVINKIDLPSAQPDVVKAEIEEMIGLDASEAVLASAKSGIGIEEILEV",
    "response#3": "DVRNIGIAAHIDAGKTTTTERILFYTGREHKIGEVHDGAATMDWMEQEQERGITITSAATTCEWAGKQINIIDTPGHVDFTIEVERSMRVLDGAVSVFCAVGGVQPQSETVWRQRNRYGVPSIVFVNKMDRTGANFYAVEEQIRTRLKGNPVPIQIPIGEEDNFLGVIDLVKMKAIVWDVDAAMGSNYHVEEIPANLLQKAQEYREKMIESISEVDGNEHLAEKYLEGEELSEDEIIAGIKAATIGMHIVPMTAGTSFKNKGVQTLLDAVVAYLPPAPK"
  },
  {
    "reference": "MRAMERTKRSWNILSRSMVLLTLCLTFNVATDYWVTVYYGVPVWKDAETTLFCASEAKPHSSEAHNIWATQACVPIDPNPEEVFLPNVTENFSMWDNPMVSQMQEDIISLWEQSLKPCVKLTPLCVTLNCSTAIFRANRTSTNTTTPSTIATSPTTDTIYEMKNCSFNVTTELRDKKKQVYSLFYVDDVVKLNDGDSTNSSTYRLINCNTTAITQACPKISFEPIPIHYCAPAGFAILKCNDTNYLGTGKCHNVSTVHCTHGIRPAVTTQLILNGSLAEGNVTIRVKNASSNSGNYIVQLARSVEILCRREGNNTRGQVQIGPAMTFYNIENVVGDTRKAYCTLNGSNWEEAMSKVKKELEKITNVTNITFADQRQGSDPEVENHMFNCGGEFFYCNTSKLFNNKTGRGGNKSEEIILPCRIKQIVNLWTRVGKGIYAPPIRGNISCKSNITGILLARXEGKDNQNNLTFRPIGGNMEDLWRSELYKYKVVRIEPLAVAPSKAKRRTVETEKRQKRAAFGLGALFLGFLGAAGSTMGAAAVTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLSIWGVKQLQGRLLAVERYLQDQQILGLWGCSGKAICYTTVPWNKTWXGNYSYEYIWNNLTWEQWDVHVSNYTGVIFGLLEEAQGQQEKNERELLELDKWSSLWSWFBXTHWLWYIKIFIMVVAGIVGLRICMIVISVIRRVRQGYSPLSLQTLLPVQRGPGVLDGTDEEGGEQGRGRSVRLVTGCLPLIWDDLR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should contain one or more Retroviral envelope protein GP41-like, Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains that are essential for its function.2. Incorporating a YXXL motif; contains endocytosis signal, Di-leucine internalization motif motif is critical for the successful design of the protein.3. The ideal protein would be one that plays a critical role in clathrin-dependent endocytosis of virus by host cell, actin filament reorganization, positive regulation of plasma membrane raft polarization, viral protein processing through structural molecule activity within host cell plasma membrane, viral envelope, host cell endosome membrane, virion membrane, plasma membrane.4. Design the protein with a signal peptide incorporated.5. For general function, the protein need meet that Transmembrane protein gp41: Acts as a class I viral fusion protein; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane The designed protein sequence is ",
    "response#1": "KGXMKNCSFNVTTSMNKMQXERALFYTLDLAXLXNXSYRLISCNXXXXTQACPKVSFEPIPIHYCAPAGFAILKCNNKTYEGKGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEXXIIIRSDNFTDNAKTIIVQLNETVKINCTRPNNNTRRSIHMGPGKAFYTTGDIIGDIRQAHCNLSRTQWNNTLKQIVIKLREQFVNKTIVFNHSSGGDPEIVMHSFNCGGEFFYCDTSQLFNSTWLSNSTWNNTEGSNNNDSTITLPCRIKQIINMWQGVGRAMYAPPIRGQIXCLSNITGLLLTRDGGNVSESQTTEIFRPGGGDMRDNWRSELYKYKVVRIQPLGIAPTKAKRRVVQREKRXXXXXXXXXXXXXXXXXXXXXXLWVTVYYGVPVWRDAETTLFCASDAKAYDKEVHNVWATHACVPTDPEPQEINLENVTEDFNMWKNNMVEQMQTDIISLWDQSLKPCVKLTPLCVTLNCVEPNITSSNSSSVNSNNSSVNNNRLEEMKNCSFNMTTELRDKKEKVHALFYRLDIVSLGKKDSNNSGSEQYRLINCNTSAMTQACPKVTFEPIPIHYCAPAGFAILKCKDTTFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEQVMIRSQNITDTSKTIIVQLTEPVNITCIRNGSNTRTSIRIGPGQAFFATGAVTGDIRKAYCIVNGTAWNSTLQKISTQLENHFKNKTITFKNSSGGDLEVTTHSFNCGGEFFYCNTTALFNSIWNGNDNGTNSTEDTDPIILPCRIKQIVNMWQRVGQAMYAPPIPGNISCTSNITGLLLTRDGINGSNETFRPIGGDMRDNWRSELYKYKVVKIEPIGVAPTKAKRRVVEREK",
    "response#2": "MRVKGIKRNYQPLWRWGTLMLGLVITCSATDKKWVTVYYGVPVWREAKTTLFCASDARAYEKEAHNVWATHACVPTDPDPQEVPLENVTENFNMWKNNMVDQMQEDIISLWDQSLKPCVKLTPLCVTLNCTNLNVTNSNSTSSNSTSTLENGQEIKNCSFNTTTELRDKKQKVHALFYRLDIIPLNENSSEYRLINCNTSAITQACPKVSFDPIPIHYCTPAGYALLKCNDKSFNGTGPCRNVSSVQCTHGIKPVVSTQLLLNGSLAEEEVIVRSENLTNNVKTIIVHLNKSVEIVCTRPNNNTRKSVRIGPGQTFYATGDIIGDIRQAHCNISRRDWEETIHNISSKLAEXFQNKTINFTSSSGGDLEITTHSFNCGGEFFYCNTSDLFNSTYMNGTFTFNGIESNSTTESNLTITIPCRIKQFINMWQEVGRAMYAPPIAGNITCKSNITGLLLVRDGGESNDTTETFRPGGGDMRDNWRSELYKYKVVEIKPLGVAPTGAKRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLQDQQLLGIWGCSGKLICTTAVPWNASWSNKSLSAIWNNMTWMEWDKEINNYTQIIYNLIEQSQTQQEKNEQELLELDKWANLWSWFDITNWLWYIKIFIMIVGGLIGLRIVFTVLSIVNRVRKGYSPLSFQTRLPAQRGPDRPEGIEEEGGERDRDRSGRLVDG",
    "response#3": "MKVKGIQRNWKQWWTWGILGFWMLMICNVVGNKWVTVYYGVPVWREAKTTLFCASDARAYEKEAHNVWATHACVPTDPDPQEVPLENVTENFNMWKNNMVDQMQEDIISLWDQSLKPCVKLTPLCVTLNCTNLNVTNSNSTSSNSTSTLENGQEIKNCSFNTTTELRDKKQKVHALFYRLDIIPLNENSSEYRLINCNTSAITQACPKVSFDPIPIHYCTPAGYALLKCNDKSFNGTGPCRNVSSVQCTHGIKPVVSTQLLLNGSLAEEEVIVRSENLTNNVKTIIVHLNKSVEIVCTRPNNNTRKSVRIGPGQTFYATGDIIGDIRQAHCNISRRDWEETIHNISSKLAEXFQNKTINFTSSSGGDLEITTHSFNCGGEFFYCNTSDLFNSTYMNGTFTFNGIESNSTTESNLTITIPCRIKQFINMWQEVGRAMYAPPIAGNITCKSNITGLLLVRDGGESNDTTETFRPGGGDMRDNWRSELYKYKVVEIKPLGVAPTGAKRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKIICTTAVPWNSTWSNKSYEEIWDNMTWIEWEREISNYTSQIYKVLTESQDQQDRNEKELLELDKWASLWSWFDITKWLWYIRIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTLIPNPGGPGRL"
  },
  {
    "reference": "MGNENSTSDHQRTSSVQSPRSLQPPGKSQSLQKQQGDLPGSCAGSIPGTDDVIQPAAPVDPGHPPLAGIGSNQGEVCTSLQLSYTIVTVQSASPSAARASPAPLAPEHTASAPSAAGPGVEVTPTGSPQHLAKNEPRSSDSEEAFETPESTTPVKAPPAPPPPPPEVTPEPEVIDPPAPEEPGCISEPPVVVPDGPRSSESVEGSPFRPSHSSSAVFDEDKPIASSGTYNLDFDSIELVDNFQSLEPCSADSKGQECKVSTRRKSTESVPPSKSTLSRSLSLQASDFDGASCPGSPEAGTLTTDACGTGSNSASSTLKRTKKTRPPSLKKKQATKKPTETPPVKETQQEPGEESPVPSEEHLAPETKTESATPEGAGCTLSDDTPLESPAVPTATCPLTLESAEDVSPLVSGGGRVQNSPPVGRKSVPLTTASEAVEVTLSDSGGQEDLPAKGLSVRLEFDYSEDKGSWESQQENAPPTKKIGKKPVAKMPLRRPKMKKTPEKLDNTPASPPRSPTEPSDTPIAKGTYTFDIDKWDDPNFNPFSSTSKMQESPKLSQQSYNFDPDACEESLDPFKASSKTPSSPSKSPASFEIPASTTEADGDGLNKPAKKKKTPLKTMVEDVMSVCSLFDTFRVKKSPKRSPLSDPPSQDPTPAATPEAPSAISTVVHATDEEKLAVTSQKWTCMTVDLDADKQDFPQPSDLSNFVNETKFNSPSEELDYRNSYEIEYMEKLGSSLPQDDDTPKKQALYLMFDTPQESPVKSPPVRM",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be involved in interkinetic nuclear migration, cell population proliferation, microtubule cytoskeleton organization by localizing to nucleoplasm, microtubule organizing center, cytoplasm, cytosol and exhibiting nuclear receptor binding.2. For general function, the protein need meet that May play a role in organizing centrosomal microtubules .; Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors3. The designed protein must have at least one SPAZ domain.4. The designed protein's functionality will benefit from Pro residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "DTQKKTFTCWINSQLAKHAPPSVISDLFTDIKKGHVLLDLLEVLSGQQLPRDKGSNTFQCRINIEHALTFLKNRSIKLINIHVADIIEGNPSIILGLIWTIILHFV",
    "response#2": "CSPAPSGKTVSSVSQDVTELDFETNTFCPPPLYYTHLTQEKTPSARVEITIEPQRNEPEDLDDIFPETKLVSPPLRPVKHTRAAVQESPPVLPNLPQTQGPGEANEAPCPPQIEQSPVNAIRQLPLLNALLIELSLLCNQPVASPTQVHPHLAWLYRGEDKGPDPSTKSTSPSESKSNKLSVRENEKLVNPQSKKNPKGKHPKIGGSPPPKVTKGRLLYGLTNTLRLRLQQTNPNMLVVHEKREQYRRSQIQAVGPKLRIPSWKGKVSSSAAESQMSPQLPADTPTDSNC",
    "response#3": "CSPAPSGKTVSSVSQDVTELDFETNTFCPPPLYYTHLTQEKTPSARVEITIEPQRNEPEDLDDIFPETKLVSPPLRPVKHTRAAVQESPPVLPNLPQTQGPGEANEAPCPPQIEQSPVNAIRQLPLLNALLIELSLLCNQPVASPTQVHPHLAWLYRGEDKGPDPSTKSTSPSESKSNKLSVRENEKLVNPQSKKNPKGKHPKIGGSPPPKVTKGRLLYGLTNTLRLRLQQTNPNMLVVHEKREQYRRSQIQAVGPKLRIPSWKGKVSSSAAESQMSPQLPADTPTDSNC"
  },
  {
    "reference": "MSVAERTASQRTLAALLNGLVSGRVPALPITDISARSSAVEQGGMFLACRGIHSHGLDHLPEALAAGAAAVIWEPTEGLVAPAVPGGVISLPVERLGAHAGEIASRFFGAPSQRLRIAGITGTNGKSTCAHLIATSLERRGRACGFLGTLGFGRPDALQSAALTTVDAVTLQRRLADLDASAVRYAAMEVSSHALDQGRVVGVHFDTAVFTNLSRDHLDYHGNRHSYGAAKARLFTHCDVERAVINAGDAFGREILDRLAARLEPVVVALCPVPEPSLRAGGGGATQARRHLQGTLIRSDKRGVQIRFTGSWGAGTLSSGLIGEFNAENLLLALAVLLVWELPLDEALAGLAGASTAPGRMERFGGGDGPLAIVDFAHTPAALARALEASRAICDGQVWCVFGCGGERDRGKRGLMGEIAEQHADRVIITDDNPRGEDAERIVRDIQSGMANPSAASIERDREAAIAFALSSAQSGDVVVVAGKGHEDYQLVGSERRAFSDREVVARLVGGRR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should take into account the physiological conditions of the Cell wall biogenesis, peptidoglycan biosynthesis pathway.2. The designed protein should have magnesium ion binding, ATP binding within cytoplasm to regulate peptidoglycan biosynthetic process, cell cycle, cell division, cell wall organization, regulation of cell shape.3. The protein should have a low Km value for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.4. The protein must bind to Mg(2+) in order to perform its enzymatic function.5. The designed protein must have at least one Mur ligase C-terminal, Mur ligase N-terminal catalytic, Mur ligase central domain.6. Incorporating a Meso-diaminopimelate recognition motif motif is critical for the successful design of the protein.7. The designed protein should be able to compete with endogenous proteins or inhibitors for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding. The designed protein sequence is ",
    "response#1": "MTDIRELTLDSRKVRPGDLFLAVPGAQVDGREHIADALKRGAAAVAYEAQGTTVLPITEVPLIPVKGLIAQLSDIAGRFYNYPSRKLKLVGVTGTNGKTTTSRLVAQLMRALGKKVGVIGTINYRYQGKLIETDRTTPDPIDLQRLLAEMVSAGCTHVVMEVSSHALELDRVYGCEFDVGVFTNLTQDHLDFHKTMEDYFQAKCRLFENLKPRGGKIAIINADDPYGRRLVGMAGCRWITYGMEKEADYRATDIGLSPEGTRFLLVTPQGEIPISLKLVGKFNVYNALAAIAAGVSEGIPLETIKRGIESVSQVP",
    "response#2": "MKLKDLIAGLTAQPVPGDNVESIAYDSRRVQRNGMFVALRGEKSDGHQFIGQAIEKGASVIVVEHEGSHPRATFLVVENTRAALADLSAAFYGNPSQKLLLVGVTGTNGKTTTSRLVAQLMRALGQKAALIGTIEFRDGTRQLSSRTTPEPHDLQEWFQQMAAQGVTAAVMEVSSHALELDRVYGCEFEVAVFTNLTQDHLDYHGTMEAYGQAKKRLFTGLGQGEGSKRFAVINLDDPAGPRMLELAGCEVWTYGIHSGADLRAEDIKATLSGGRMQAIENSLGFQVVVDYAHTPDALEQALVALRPYVSGQLITVFGCGGDRDPGKRQVMGRIACARSDRVVVTSDNPRGEDPAAILADIETGCSGKYMLEVDRKKAIEAAVGLARPGDCVVIAGKGEEAYQILHRGEKIAFSTREEVLLALSQHKK",
    "response#3": "MKLPKEITGLTLDNRQVQPGMAFVALRGTRQHGLDYAEAAVRAGASVVLYEPEIGLQPPVLSVDAVSVPALREHLGTLADRFYGFPSQKLKLIGITGTNGKTTTCYLARYFLEKAGVPTGIIGTVAYEFEGRSIPARRTTPDIFELNGLIRNMCDSGAEIVMMEVSSHALDQNRMGNLKYDVAIFTNLSRDHLDYHKTMEEYFSAKRKLFNKLKTEVSSAKSRAIISLDDQWGKKLFGEIHEKNLRLITYSSDYKSKAMVKAENIIASTSGTSFDLIVDDNKIACVEIPLIGHHNVSNCLAAMVIAKLSGISLERSLESLSTFRGVPGRVEMVKVSDDFILLIDYAHNAMALSSILTTLREYHPGRLVCVFGCGGNRARSRRYEMGEVSGRMADFTIITSDNPRYENPEAIMDDIEVGMKKTTGKYIRIADRKEAIAYAIHHGKPGDIIVLAGKGHEDYQILGTEKIHFDDREIARAAVKERSL"
  },
  {
    "reference": "MNMKLVFATNNKNKIKEIKLLLDDSIELLSLEDINCTEGIPETSDTIAGNALQKARYVFDNYGCNCFADDTGLEIDSLNGAPGIYSARYAGEKKSADANMDKVLTKLKHINNRKAHFKTVIALIISGKEALFEGIAKGKITTIKSGSEGFGYDPIFKPEGYNVTFSEMSLEKKNKISHRGIAVRKLAKHLTSL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should be able to enhance the efficiency of nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "PWAYKMQRGRVAVFAGSSSGAGAALHCIHGVASAGAGYIALFCDKELFSAYLASIGENAIVRVFSKDSFSPECWDVVIAGPGWGIDLERERILAMLFQTDIPLVLDADGARMFARIASKNQDSSFCRAPIILTPHPGEFSEMLPILKSVPLDPLANVSETVALVTAFAKKYGTTLALRASTTHVGLPDGSCYIYDGSTPGLGIAGSGDVLSGMIGGFLARWLAFLKETGENRESTNAAIPSDNPMTKALLGAILVHGSAGKQLWLKKGWFTPIDLANS",
    "response#2": "LWDMYFINLVERQNIWYRRVIQGKSKPWTDDPVMSTYHFTNVDRRLDRVTLHYIDKVLCNLEDSYESKKFLLLNTFIYRLFVRPETTDAMGYIFPETFEEDWERAKAALRARRESGEPVFTDAYFVNDLKSANPDRANSSNKTENAIHLIQFIIDHLDELAEFTFNPKNSMEEVIEKFTMIPAVGNFNAYEVALDLGIVKEMTGIDFVDWTPDHYANVGPGCKKGIEYVFEDLGNMSHLDIVFFITSVYKGELERLGLEYKYQEGCKELDLRALEGWCCEMSKYFP",
    "response#3": "LWDMYFINLVERQNIWYRRVIQGKSKPWTDDPVMSTYHFTNVDRRLDRVTLHYIDKVLCNLEDSYESKKFLLLNTFIYRLFVRPETTDAMGYIFPETFEEDWERAKAALRARRESGEPVFTDAYFVNDLKSANPDRANSSNKTENAIHLIQFIIDHLDELAEFTFNPKNSMEEVIEKFTMIPAVGNFNAYEVALDLGIVKEMTGIDFVDWTPDHYANVGPGCKKGIEYVFEDLGNMSHLDIVFFITSVYKGELERLGLEYKYQEGCKELDLRALEGWCCEMSKYFN"
  },
  {
    "reference": "MNEIISTTILLILIMDPLGNLPIFMTILKKLDAKRRRIVVIREMIIALIVMLIFLFVGEKILTILNLKTETVSISGGIILFLIAIKMIFPSDEGNNGTSSEEEPFLVPLAIPLVAGPSLLATLMLLSHQYLHHMPYLVGSLLIAWFFTIIILLLSGLFLKLFGDKGVNALERLMGLILIMLSTQMFLDGIKAWFKN",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should contain a Helical membrane-spanning region.2. The designed protein should be expressed specifically in the plasma membrane The designed protein sequence is ",
    "response#1": "IVNQYEGGLIFRLGRVIGKLKPGINIIIPFLDVPVKVDMRTRVTDIPPQEMITKDNAVVKVDAVVYYRVIDVEKAILEVEDYEYAIINLAQTTLRAIIGSMELDEVLNKREYINSKLLEILDRETDAWGVRIEKVEVKEIDPPEDIKNAMAQQMKAERLKRAAILEAEGEE",
    "response#2": "IVNQYEGGLIFRLGRVIGKLKPGINIIIPFLDVPVKVDMRTRVTDIPPQEMITKDNAVVKVDAVVYYRVIDVEKAILEVEDYEYAIINLAQTTLRAIIGSMELDEVLNKREYINSKLLEILDRETDAWGVRIEKVEVKEIDPPEDIKNAMAQQMKAERLKRAAILEAEGEN",
    "response#3": "IVNQYEGGLIFRLGRVIGKLKPGINIIIPFLDVPVKVDMRTRVTDIPPQEMITKDNAVVKVDAVVYYRVIDVEKAILEVEDYEYAIINLAQTTLRAIIGSMELDEVLNKREYINSKLLEILDRETDAWGVRIEKVEVKEIDPPEDIKNAMAQQMKAERLKRAAILEAEGER"
  },
  {
    "reference": "MLPLIIFVLLCGFYTSWNIGANDVANAVGPSVGSGVLTLRQAVVIAAIFEFFGALLLGDRVAGTIESSIVSVTNPMIASGDYMYGMTAALLATGVWLQLASFFGWPVSTTHSIVGAVIGFGLVLGKGTIIYWNSVGIILISWILSPFMGGCVAYLIFSFIRRHIFYKNDPVLAMVRVAPFLAALVIMTLGTVMISGGVILKVSSTPWAVSGVLVCGLLSYIITFYYVHTKHCSYISDTPKKGSLTYRLKERGGNYGRKYLVVERIFAYLQIIVACFMAFAHGSNDVANAIAPVAGVLRQAYPASYTSYTLIRLMAFGGIGLVIGLAIWGWRVIETVGCKITELTPSRGFSVGMGSALTIALASILGLPISTTHVVVGAVLGIGLARGIRAINLNIIKDIVLSWFITLPAGALLSILFFFALRALFH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The designed protein should be optimized for inorganic phosphate transmembrane transporter activity activity in order to improve phosphate ion transport. The designed protein sequence is ",
    "response#1": "PVSVGAALVIVERLPKRLAEAVGIVLELLAGIPSVVVGLWGAMTFGPFIAHHIAPVIAHNAPDVPVLNYLRGDPGNGEGMLVSGLVLAVMVVPIIATTTHDLFRQVPVLPREGAIALGMSNWECVRRVTLPWVSSGIVGAVVLGLGRALGETMAVAMVSGAVLGAMPANIYATMTTIAATIVSQLDSAMTDSTNFAVKTLAEVGLVLMVITLLTNVAARGMVRRVL",
    "response#2": "VLTGIYLSEFATGKTRSILRGAYEVLSGIPSIVLGYVGYLALVVYFDWGFSLAAGVLVLSVMSIPYIAKATESALAQVPTSYREAAEALGLPAGWALRKIVLKTAMPGIVTGMLVALALAIGETAPLLYTAGWSNSPPTGQLTDSPVGYLTYPIWTFYNQPSKSAQDLSYDAALLLIVFLLLLIFIGRLINWLSRT",
    "response#3": "PVSVGAALVIVERLPKRLAEAVGIVLELLAGIPSVVVGLWGAMTFGPFIAHHIAPVIAHNAPDVPVLNYLRGDPGNGEGMLVSGLVLAVMVVPIIATTTHDLFRQVPVLPREGAIALGMSNWECVRRVTLPWVSSGIVGAVVLGLGRALGETMAVAMVSGAVLGAMPANIYATMTTIAATIVSQLDSAMTDSTNFAVKTLAEVGLVLMVITLLTNVAARGMVRRVY"
  },
  {
    "reference": "MLLAVPSKGRLQEPTLKLLEAVGIKPLASDERALVLPTSWPDVNLIKARPEDIPYLVESGRVWAGVTGHDYVLESGSNVVEALDLEFGRGRLVVAVPKSSGIKSVEELPPGARVATKFVNIAYNYFAELGKRVRVVKVTGSVEVLPQLGIADAILDVMATGTTLEVHGLTPIATVLETSARLVVHPEYTSHELTKKLVTFIKGYFAAQGKKMIFLNVPAARLEAVLSVLPAMEAPSVTKLAKGDIYEVFSVVSEDVLPDLVMKLKNAGAKDIVVTSIEKLIS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. Mg(2+) is required for the proper folding and stability of the protein.2. The desired protein should be able to perform 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-   alpha-D-ribose 1-diphosphate + ATP3. A protein that is capable of catalyzing reactions within the Amino-acid biosynthesis, L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9, L-histidine biosynthesis pathway with high efficiency and specificity.4. The protein can be targeted to cytoplasm and perform ATP binding, ATP phosphoribosyltransferase activity. The designed protein sequence is ",
    "response#1": "RTHDVPVYVEYGQAQLGFAGYDVLREKKPDVANLLDLGFGGCRLSVAVPKASPYKNPRQLPPNCRVASKFVNCAKDYFRDLDLPIEVIPLHGSVELGPITGMSEAIVDLVSTGNTLRENNLEEVEVLFHSTVRLIAHPTSYRANRHNMLDWVQKLG",
    "response#2": "RTHDVPVYVEYGQAQLGFAGYDVLREKKPDVANLLDLGFGGCRLSVAVPKASPYKNPRQLPPNCRVASKFVNCAKDYFRDLDLPIEVIPLHGSVELGPITGMSEAIVDLVSTGNTLRENNLEEVEVLFHSTVRLIAHPTSYRANRHNMLDWVQKLR",
    "response#3": "RTHDVPVYVEYGQAQLGFAGYDVLREKKPDVANLLDLGFGGCRLSVAVPKASPYKNPRQLPPNCRVASKFVNCAKDYFRDLDLPIEVIPLHGSVELGPITGMSEAIVDLVSTGNTLRENNLEEVEVLFHSTVRLIAHPTSYRANRHNMLDWVQKLK"
  },
  {
    "reference": "MQAAKKDIRALTKEQLRDFFVSQGDKAFRGNQVYEWLWGKAAYSFEAMTNISMETRKMLEDNFVINHIKVDQMQRSNDGTIKNAVRLHDDLIVESVLIPTASRTTACVSSQVGCSLDCKFCATAKLKRMRNLNPDEIYDQVVAIDNESRLYFDKPLSNIVFMGMGEPLMNYNNVLAAIEKITSPEGLGMSPRRITVSTSGVPKMIKKMADTEVKFKLAVSLHSAIDEVRTSIMPFNATFPLADLREALQYWYDKTKSRITYEYVVWRGINDTRKDADALVKFCKFAPSKVNIIEYNPIGDEVFNQADTSAIEMYQQILERNGITVTVRRSRGKDIDAACGQLANKS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The S-methylcysteine intermediate, Proton acceptor of the protein must be engineered to enable effective catalysis of adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.2. The protein contains novel Radical SAM core domains that confer a unique function or activity.3. The presence of [4Fe-4S] cluster is necessary for the protein to function in the desired environment.4. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.5. The [4Fe-4S] cluster, S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand.6. The designed protein should be involved in rRNA base methylation by localizing to cytoplasm and exhibiting rRNA (adenine-C2-)-methyltransferase activity, tRNA (adenine-C2-)-methyltransferase activity, rRNA binding, 4 iron, 4 sulfur cluster binding, metal ion binding. The designed protein sequence is ",
    "response#1": "KTACVSSQIGCSLSCKFCATGYMERERNLEFDEIYDEVVLINQQSERIYDKKLSNIVFMGMGEPLLNYKNVLRAIEKISSPDGLGMSQRRITVSTAGVAKMIRQLGDDQVRFKLALSLHAANDQKRHAIMPINDTNNIRSLIDALNYFYKQTGNEITFEYILFRNFNDSTKDADELVKIYRQVPADLVNIIEYNPIDKALFEKPDDETVEKFMNRFDAAALRSKPSLIGLDRVALADRLTDLGVPERQIRMRVAQLWNRLYVGGAQTFDDMTTLSKDLRHALDSQVSLDRPEIVSEQISTDGTRKWLLRLAPGRPGEAAPEIETVYIPEEDRGTLCVSSQVGCTLTCTFCHTGTQRLVRNLTPGEIVGQILIARDRIGEWPGATRPTDGPLPNAERLITNIVLMGMGEPLFNFDNVRQAMEIAADGDGISVSKRRITLSTSGIAPMIPRCGVELGVMLAISLHAVRDDLRDVLVPINRKYPIAELLDACRAYPSLSNAKRITFEYVMLRGVNDSLADAKALVRLLAGIPAKINLIPFNPWPGAPYESSEWEQIEKFAEIINKAGYASPVRTPRGRDIMAACGQLKSASEKMHARKQATNID",
    "response#2": "EDKRNTVCVSTQVGCALGCTFCRTGSMGFVRNLSSGEILSQLLLISRLSANPVTNVVFMGMGEPFLNFDALIEAAKMMNDPRAFNLSARRISISTAGIIPGIEQLSLLPWQFKLAISLNAIFPELRETLMPISKKYSLDNLLKAAKSYTEKSGKLVMFEYVLIDGVNDRDEDAAELKRRLKDIPCKINLIPFNSIATAMQRPSPQRIRRFISNLENAPFPVIVRHSGGTSED",
    "response#3": "KTACVSSQIGCSLSCKFCATGYMERERNLEFDEIYDEVVLINQQSERIYDKKLSNIVFMGMGEPLLNYKNVLRAIEKISSPDGLGMSQRRITVSTAGVAKMIRQLGDDQVRFKLALSLHAANDQKRHAIMPINDTNNIRSLIDALNYFYKQTGNEITFEYILFRNFNDSTKDADELVKIYRQVPADLVNIIEYNPIDKALFEKPDDETVEKFMNKPLIVEEKAKKNIRQLTFEELQSYFESMGEKKFRAKQVYEWLWQKHAHSFDAMSNLSKDMRKLLGETFSLPALHIESTQYSADGTVKSRFKTFDQHFVEGVLIPTDERKTACVSSQIGCSLSCKFCATGYMERERNLEFDEIYDEVVLINQQSERIYDKKLSNIVFMGMGEPLLNYKNVLRAIEKISSPDGLGMSQRRITVSTAGVAKMIRQLGDDQVRFKLALSLHAANDQKRHAIMPINDTNNIRSLIDALNYFYKQTGNEITFEYILFRNFNDSTKDADELVKIYRQVPADLVNIIEYNPIDKALFEKPDDETVEKFMNHLEKNKVNARLRRSRGKDIDAACGQLANKS"
  },
  {
    "reference": "MCHSKEVKFKTNFHHPYTPYDIQLEFMRSLYSSISDGKIGIFESPTGTGKSLSLICASLTWLDEHGGVLLEDNEKSNDNKSNTSSKIPDWVLEQDLKIQKDLVKETHARLEQRLEEIRKRNQSRKNQMSNNSTTYHRETKRRNINAEASTSDNCNNSNTSVDPMDEYLVTAEYTMPSTSEQSEDLFNNGYSSKVSELLRKLSPDNEKPPIVQKIYFTSRTHSQLQQLVQEIKKLNNQTFSTPIRVVSLASRKNLCINNEVRKLRPTSALNEKCIELQGSAHKCPFLQDNTQLWDFRDEALAEIMDIEELVELGQRLKVCPYYGTREAVDSAQIVTLPYPLLLQESARNALNLTLKDNICIIDEAHNLIDAICSMHSSSISFRQVCIAETQLQQYFLRFEKRLNGNNRMHIKQLIKVVYNLKSFFLNCLETNTNSKVINVDSLLVSNGADQINLHHLSEYLNVSKLARKVDGYTKYMHSLGTQELESLNDLRSERFSNGNGYEEDPYTPVLMQLESFLLNIANPAPEGKLFYEKQTGDNPYLKYLLLDPSKHVEILTEQCRSVNLAGGTMSPIDDFITLLFSDEQSRILPFSCDHIVPPENITTILVSQGPAGVPFEFTHKRKDDENLLKDLGRTFQNFISIIPDGVVVFFPSFAFLQQAVKVWEMNGITNRLNAKKPLFIESKDFGDNPLDTFEHYKQSVDAGLSGMLFSVIGGRLSEGINFSDKLGRAVMVVGMPFPNSQDVEWQAKVSYVEEKAKEKGINAKQASQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: ATP-dependent DNA helicase important for chromosome transmission and normal cell cycle progression in G(2)/M ; Has a specific role in chromosome segregation during meiosis II .; May have a role in changing DNA topology to allow the loading of proteins involved in maintaining sister chromatid cohesion in the vicinity of the centromeres .2. The protein design should emphasize Polar residues compositional bias.3. A protein that can perform DNA helicase activity, DNA binding, ATP hydrolysis activity, ATP binding in nucleus, site of double-strand break, cytosol to support establishment of sister chromatid cohesion, interstrand cross-link repair, DNA duplex unwinding, DNA-templated DNA replication maintenance of fidelity.4. The protein should have a fast turnover rate for ATP + H2O = ADP + H(+) + phosphate. The designed protein sequence is ",
    "response#1": "MCQKYDTLSGDRDVEHDEHFVLEDYDSESEDQNISSKSLGHSSELSMSTLALLERFKKQYSAPTEEEETVGDDEPKIFYCSRTHSQLAQFASELRRVEMPPSLPKELSEKLTDSEALEERVKHLSLGSRKNLCINPRVMKLENATAINERCMDLQQPGMAVEHRCPYLPSKDDEPQVLQFRDHTLATVKDIEDMGKLGKHIGVCPYYASRSVIKHSEIVTLPYPLLLQRSAREALGLSIKNHIVIIDEAHNLMDAISNIHSVTITLSQLQTAIFQLTTYARKFKTRLKGKNRTYIAQVYCSRTHSQLAQFASELRRVEMPPSLPKELSEKLTDSEALEERVKHLSLGSRKNLCINPRVMKLENATAINERCMDLQQPGMAVEHRCPYLPSKDDEPQVLQFRDHTLATVKDIEDMGKLGKHIGVCPYYASRSVIKHSEIVTLPYPLLLQRSAREALGLSIKNHIVIIDEAHNLMDAISNIHSVTITLSQVGAKPWSLQRVWAKRVFMNQSFAIVAPTGVGKTTFGLVMSLFLKGRVLAIFPTRLLAQQAGDRLSELAQKVGVNKKILIYQSKKNIREQFLNGDWDILLGTNMFLHKNFENLINFKFKLIFIDDIDSFLKRGKNVDYLFKLLGFSGEEIKLALKENKTQRDYDRLARIRKRKRDTVLIVSSATLKPRGKRAYLFRNLLGFDVQKAITTLRNIVDYKYLDEIIVSWRKAGLFEKLDKERKVFYEMKDGPDPLPDYTSAVANSEGAILFAIVGGKLSEGINFGGNLCRAIVMTGLPYPNVFSGELIISRKHLEEKVLNGGGSKTDANMAAKEFFENICMKAVN",
    "response#2": "MCQKYDTLSGDRDVEHDEHFVLEDYDSESEDQNISSKSLGHSSELSMSTLALLERFKKQYSAPTEEEETVGDDEPKIFYCSRTHSQLAQFASELRRVEMPPSLPKELSEKLTDSEALEERVKHLSLGSRKNLCINPRVMKLENATAINERCMDLQQPGMAVEHRCPYLPSKDDEPQVLQFRDHTLATVKDIEDMGKLGKHIGVCPYYASRSVIKHSEIVTLPYPLLLQRSAREALGLSIKNHIVIIDEAHNLMDAISNIHSVTITLSQLQTAIFQLTTYARKFKTRLKGKNRTYIAQVKIAEMKQTDQEQLQNEYLKLVEGLRDAGEARQEDAFMANPDDLLNEAVPGNIRRAEHFVAFLKRFIEYLKTRMKVRNTISETPPSFLAHLKEFTFIEKKPLKFCAERLTSLVRTLELTNIEDYQPLQEVATFATLVATYEKMCQKYDTLSGDRDVEHDEHFVLEDYDSESEDQNISSKSLGHSSELSMSTLALLERFKKQYSAPTEEEETVGDDEPKIFYCSRTHSQLAQFASELRRVEMPPSLPKELSEKLTDSEALEERVKHLSLGSRKNLCINPRVMKLENATAINERCMDLQQPGMAVEHRCPYLPSKDDEPQVLQFRDHTLATVKDIEDMGKLGKHIGVCPYYASRSVIKHSEIVTLPYPLLLQRSAREALGLSIKNHIVIIDEAHNLMDAISNIHSVTITLSQLQTAIFQLTTYARKFKTRLKGKNRTYIAQVVKILLAHYCRTGNNLWPAQRLWAKRLLQGESFAIVAPTGVGKTTLLEVYALWLAGSHLRTYYVVPTQNLVEQVYNKLLMLAKRANYDLKIVAYISSRRTDKHKVMEAINNADYNILITTSQFMLKYYKLIASTSPELVVVDDVDAVLRSPKSVVKTLTIVGFTEEAVKLAIDIERLRIDALAALAKGRKKQAEKIYERIVELETKLADELLKSSPGQLIIASATGRAYGVHSKAFNRLLGFDVGRMIDTIRDVANFYEV",
    "response#3": "MYDPLKYPNPALQWHYRILQALALEEEVPEKADDATEPKYKAISKRAGGYLEEWSESLEEESGKVANKKSTKRETDDEDMDRPVKK"
  },
  {
    "reference": "EGSGGSGSSGNFTTGSNIHMSSVTNTSNAGTGTSGTGNSGGGSGGGTGPGSGAIPPVTLTESLLNK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.2. For general function, the protein need meet that Essential for the circadian rhythmicity of locomotor activity, eclosion behavior, and for the rhythmic component of the male courtship song that originates in the thoracic nervous system; Behaves as a negative element in circadian transcriptional loop; Determines the period length of circadian and ultradian rhythms; an increase in PER dosage leads to shortened circadian rhythms and a decrease leads to lengthened circadian rhythms; The biological cycle depends on the rhythmic formation and nuclear localization of the TIM-PER complex; Does not appear to bind DNA, suggesting indirect transcriptional inhibition .; Essential for biological clock functions; Nuclear activity of the heterodimer coordinatively regulates PER and TIM transcription through a negative feedback loop; Light induces the degradation of TIM, which promotes elimination of PER3. The protein should be designed to localize specifically to perinuclear region of cytoplasm, nucleus in order to perform its role in rhythmic process more effectively. The designed protein sequence is ",
    "response#1": "QMDGKILQTFEKSFVQVQNILDHNRLLINEINQNHESKIPDNLGRNVGLIRELNNNVRRVAHLYVDLSNNFSKSMEASSEGLTYVVSPNALAILEEINHKLTYEDQTLRTFRRAIGFGQNVRVDLIPLLENAKDDAVLESVIRILVNLTVPVECLFSVDLMYRTEVGRHTIFELNKLLYNSKEAFTDPKSTKSVVEYMKHILESDPKLSPHKCDQINNCLLLLRNILHIPETHAHFLMPRLQPGSGHQVSMQNTILWNLFIQSIDKLLLYLMTCPQRSLWGVTMVQLIALIYKDQHVSTLQKLLNLWFEASLSESSDDNESNTTPPKQASGDSSPMLTSDPTTYVVSPNALAILEEINHKLTYEDQTLRTFRRAIGFGQNVRVDLIPLLENAKDDAVLESVIRILVNLTVPVECLFSVDLMYRTEVGRHTIFELNKLLYNSKEAFTDPKSTKSVVEYMKHILESDPKLSPHKCDQINNCLLLLRNILHIPETHAHFLMPRLQPGSGHQVSMQNTILWNLFIQSIDKLLLYLMTCPQRSLWGVTMVQLIALIYKDQHVSTLQKLLNLWFEASLSESSDDNESNTTPPKQASGDSSPMLTSDPTGPPGSQDSVVDERAVPLLGYLPQDLIETPVLVQLHPSDRPLMLAIHKKILQAGGQPFDYSPIRFRARNGEYITLDTSWSSFINPWSRKISFIII",
    "response#2": "TYVVSPNALAILEEINHKLTYEDQTLRTFRRAIGFGQNVRVDLIPLLENAKDDAVLESVIRILVNLTVPVECLFSVDLMYRTEVGRHTIFELNKLLYNSKEAFTDPKSTKSVVEYMKHILESDPKLSPHKCDQINNCLLLLRNILHIPETHAHFLMPRLQPGSGHQVSMQNTILWNLFIQSIDKLLLYLMTCPQRSLWGVTMVQLIALIYKDQHVSTLQKLLNLWFEASLSESSDDNESNTTPPKQASGDSSPMLTSDPTVDERAVPLLGYLPQDLIETPVLVQLHPSDRPLMLAIHKKILQAGGQPFDYSPIRFRARNGEYITLDTSWSSFINPWSRKISFIIPATYVVSPNALAILEEINHKLTYEDQTLRTFRRAIGFGQNVRVDLIPLLENAKDDAVLESVIRILVNLTVPVECLFSVDLMYRTEVGRHTIFELNKLLYNSKEAFTDPKSTKSVVEYMKHILESDPKLSPHKCDQINNCLLLLRNILHIPETHAHFLMPRLQPGSGHQVSMQNTILWNLFIQSIDKLLLYLMTCPQRSLWGVTMVQLIALIYKDQHVSTLQKLLNLWFEASLSESSDDNESNTTPPKQASGDSSPMLTSDPTL",
    "response#3": "DMFAVAVSLVSGKILYISNQVAPIFHCKKDAFSDAKFVEFLAPHDVSVFHSYTTPYKLPPWSVSSGLDSFTQECMEEKSFFCRVSVGKHHENEIRYQPFRMTPYLVKVQEQKGAASQLCCLLLAAFSGS"
  },
  {
    "reference": "MSQNERDSLELERKNTPPDGPRLPERRPRFSVWIYLAIFLALLVHFFLFWTGTDTSTIEYSQFLEYVEKGYVERVEIVNDTKVQGRFTEAAVREGLVSVPVRQTDLLRGAQTPELIRRFTTTKPADHDLTSFLLAYNERARAEGRPTVQFTARIEENWFGGLLTWIFPLILIVALWVFLLRRMSPSSQVLNIGKNRAILYDAMGDHRVTFKDVAGLDEAKEEVAEIVEFLKNPKKFTRLGGKLPKGVLLVGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGAARVRDLFRQAKEKAPCIIFIDEIDAIGRSRGRGIMMGANDERENTLNQLLVEMDGFNTDKGVIIMAATNRPDVLDPALLRPGRFDRQILIDKPDRRERLEIFKVHTRDLILGDDVDLEVLAGQTPGFAGAEIANVCNEAALLAARKGKEAVEMEDFEQAIDRVIAGLEKKNKIISPEEREIVAYHEAGHAIVGWFLRYTDPVVKVSIVPRGLAALGYAQYLPEERYLYTKEALLDRMTMAIGGRVAEELVFGRISTGAQNDLERITRMAYAMVVDYGMSERVGYVSFNLSGQYGEQAFFDKPYSEETARLIDEEVRRIINEVRERARRILEEKRDKLEALARRLLEKEVLGPRDLVEILGPRPYGDYPSPNGKDVEELKDLQKGEPTSSSAVEAPAPQTERPESSSAP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be able to perform zinc ion binding under specific conditions. The designed protein sequence is ",
    "response#1": "PGELPAREPWDVVQLARHIERPHTLEYVGFVFDEFQELHGDRLFEEDHAIVGGLARLGELPLMVIGHQKGHTTGEMMERNFGMPQPEGYRKSMRLMRYAARFGLPIVTFVDTPGAYPGIGAEERGQSVAIAESIMQMSRLPIPIVTVVTGEGGSGGALALAASDRMLMLENSYYSVISPEGCATILFKDAAAAPKAAAALRMTAPDLLRLRVMDAIVREPEQGAHTDPAAAATNVKAALVQSLAS",
    "response#2": "LTVQLPTRFGDFDLHVYLDDYDDRPHLALCMGGVGQRGPDGEVLPQPEPTLVRVHSECLTGDVLGSLRCDCGAQLERAMRHVADEGRGVILYMRQEGRGIGLKNKLRAYALQDQGLDTVEANEVLGFGADMRDYGVGAQILAHLGLNRIRLLTNNPMKIIGLEGFGLEIVERVL",
    "response#3": "LTVQLPTRFGDFDLHVYLDDYDDRPHLALCMGGVGQRGPDGEVLPQPEPTLVRVHSECLTGDVLGSLRCDCGAQLERAMRHVADEGRGVILYMRQEGRGIGLKNKLRAYALQDQGLDTVEANEVLGFGADMRDYGVGAQILAHLGLNRIRLLTNNPMKIIGLEGFGLEIVERVL"
  },
  {
    "reference": "MAVWQAASGKVYLPPSTPVARVQSTDEYVQRTNIYYHAYSDRLLTVGHPYFNVYNVQGSKLQIPKVSGNQHRVFRLKLPDPNRFALADMSVYNPDKERLVWACRGIEIGRGQPLGVGSVGHPLFNKVGDTENPNSYKASSTDDRQNVSFDPKQLQMFIIGCAPCIGEHWDKALPCDDGNIQQGSCPPIELINSVIEDGDMADIGYGNLNFKALQQNRADVSLDIVNETCKYPDFLKMQNDVYGDSCFFYARREQCYARHFFVRGGKTGDDIPAGQIDEGSMKNAFYIPPNSSQAQYNNLGNSMYFPTVSGSLVSSDAQLFNRPFWLQRAQGHNNGICWFNQLFVTVVDNTRNTNFSISINSDGTDVSKITDYNSQKFTEYLRHVEEYELSLILQLCKVPLKAEILAQINAMNSNILEEWQLGFVPAPDNSIQDTYRYIDSLATRCPDKNPPKEKVDPYKNLHFWDVDLTERLSLDLDQYSLGRKFLFQAGLQQTTVNGTKTVSSRISTRGIKRKRKN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein can be targeted to T=7 icosahedral viral capsid and perform structural molecule activity.2. For general function, the protein need meet that Encapsulates the genomic DNA together with protein L2.; Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter; This binding mediates a conformational change in the virus capsid that facilitates efficient infection The designed protein sequence is ",
    "response#1": "MXPXRX",
    "response#2": "MXPXRXV",
    "response#3": "SRRRRF"
  },
  {
    "reference": "MSQKLVLILNCGSSSLKFSILDPQTGEEKLSGLAEAFHLDDARIKWKLHGEKGNADLGAGAAHSEALNFIVNSIFPLDPSLKEDIVAIGHRIVHGGEKFTSSVVITDEVVQGIKDAVQFAPLHNPAHLIGIEEAFKMFPHLKDKNVAVFDTAFHQTMPEEAYLYALPYSLYREHGVRRYGAHGTSHFFVSQQAAERLNVPAEQVNVITCHLGNGASIAAVRHGQCIDTSMGLTPLEGLVMGTRSGDIDPAIVFYLHDNLGLSVEEINTLLTKKSGLLGLTEVTSDCRYAEDNYDKEASAKRALDVFSYRLAKYIGSYMAVIGERLDAIVFTGGIGENSSLVRELTLNHLKLFGYQVDSDKNKAARFGHEGVITADNTPVAMVIPTNEELVIAQDTARLCIA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Catalyzes the formation of acetyl phosphate from acetate and ATP2. The protein design must be focused on enhancing the efficiency of acetyl-CoA biosynthetic process, phosphorylation, organic acid metabolic process. The designed protein sequence is ",
    "response#1": "LFQNFSPWELLKLIEKSLVKTFEPGDCIIGFGEPGKFLGIVINGEAEVSSITETGEKKRLTILQKGDMFGEMSLLTGEPTCADVSAFNKCDVFLLPQETFSSSLIVNPAAMKIML",
    "response#2": "LFGHLTPDELATLVAGSECLWFDDGETILDAGRDVVSLGVVIEGAVVAELAGADGPGEVVGQLGSGETFGEMGLMSGEPVPAGFRASGRARVLLVPIALFRSLIMSDPGALQHIDRHLATQADKTAIIFESDAGEVNHCSYRELHRQVCHFANGLKSLGIRQGDRVVIYMPMRIEAVVAMQACARIGAIHSVVFGGFSAKSVYERIIDAGASAVITADEQIRGGRYHPLKATVDEALAMGDTATVHSVIVFRHTGTGITWQPERDHWWHDLIAGQPDECEPAWINAEHPLFTLYTSGSTGKPKGVQHSSAGYLLGAVTSMQWVFDYHADDVFWCTADVGWVTGHSYVAYGPLAIGATQVIFEGTPTHPHAGRFWEIIQKHRITTFYTAPTAIRSLIKLGSDLPAKYDLSSLRLLGSVGEPINPEAWMWYYTVVGQSRCPVVDTWWQTETGCHMIAPAPGAISTKPGSCTLPLPGIDAAVVDETGHPVEQGKGGFLVIKRY",
    "response#3": "IVLPEGDEPRTVKAAIRCTEKGIARCVLLAPPEKVRQVAQGQGLELPEGLEIIDPETVRGKYVAPMVELRKSKGLTEPQAEAQLEDSVVLGTMMLALGEVDGLVSGAVHTTASTVRPALQLIKTAPGSSLVSSVFFMLMPEQVLVYGDAAINPDPNAQELADIAIQSADSAHAFGIPVRVAMLSYSTGESGSGEDVEKVKEATKLVRERRPELLVDGPLQYDAASVPSVGRSKAPDSPVAGRATVFIFPDLNTGNTTYKAVQRSAGVVAVGPMLQGLRKPVNDLSRGALVDDIVYTIALTRL"
  },
  {
    "reference": "MDSRNQLIVAILLTSACLIYCAQYVTVFYGIPAWKNASIPLFCATRNRDTWGTIQCLPDNDDYQEIPLNVTEAFDAWNNTVTEQAVEDVWNLFETSVKPCVKLTPLCVQMECNSTSTESSNSTSEGSTVPEILNETTSCITNNSCSDLGSEEVVDCRFNMTGLQLDKPQQYSETWYSKDVVCDTTNGTSRKCYMNHCNTSVITESCDKHYWDAMRFRYCAPPGLCLLRCNDTNYSGFEPKCPKVVAATCTRMMETQTSTWFGFNGTRAENRTYIYWHGRDNRTIISLNTHYNLTMHCKRPGNKSVLPITLRSGRVFHSRPIINERPKQAWCWFGGDWKKAMQEVKQTLVKHPRYRGTNDTQKINFTQPGKGSDAEVVYMWTNCRGEFLYCNMTRFLNWIENRAHPQRNYAPCHIRQIINTWHRVGQNIYLPPREGELVCNSTVTSIIANIDMFDNQTSITFSAEVAELYRLELGDYKLVEITPIGFAPTSEKRYSSAPQRNKRGVFVLGVLGFLATAGSAMGAASLTLSAHPGLYWAGIVQQQQQLLDVVKRQQEMLRLTVWGTKNLQTRVTAIEKYLRDQARLNSWGCAFRQVCYTTVLWENNSIVPDWNNMTWQEWEQQTRDLEANISRSLEQAQIQQEKNMYELQKLNSWDVFGNWFDLTSWIKYIQYGVYVIIGIIALRIVIYVVQLLSRLRKGYRPVFSSPPGYIQQIHIHKDWEQPDREETDEDAGNSIGDSSWPWPIAYIHFLIRQLIRLLTGLYSVCKDL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Extracellular topological domain of the protein should be designed to be flexible or rigid.2. The protein should be designed to localize specifically to host cell plasma membrane, viral envelope, host cell endosome membrane in order to perform its role in clathrin-dependent endocytosis of virus by host cell, virion attachment to host cell, suppression by virus of host type I interferon-mediated signaling pathway, suppression by virus of host tetherin activity more effectively.3. For general function, the protein need meet that The gp120-gp41 heterodimer allows rapid transcytosis of the virus through CD4 negative cells such as simple epithelial monolayers of the intestinal, rectal and endocervical epithelial barriers4. Design the protein with a signal peptide incorporated.5. Incorporating a Di-leucine internalization motif motif is critical for the successful design of the protein. The designed protein sequence is ",
    "response#1": "MGAASLTLSAQSRTSLAGIVQQQQQLLDVVKRQQEMLRLTVWGTKNLQARVTAIEKYLKDQAQLNSWGCAFRQVCHTTVPWVNDTLTPDWNNITWQEWEQRIRNLEANISESLEQAQIQQEKNMYELQKLNSWDVFSNWFDLTSWIKYIQYGVYIVVGIIVLRMVIYVVQMLSRLRKGYRPVFSSPQ",
    "response#2": "MGAASLTLSAQSRTSLAGIVQQQQQLLDVVKRQQEMLRLTVWGTKNLQARVTAIEKYLKDQAQLNSWGCAFRQVCHTTVPWVNDTLTPDWNNITWQEWEQRIRNLEANISESLEQAQIQQEKNMYELQKLNSWDVFSNWFDLTSWIKYIQYGVYIVVGIIVLRMVIYVVQMLSRLRKGYRPVFSSPS",
    "response#3": "MGATSLTLSAQSRTLLAGIVQQQQQLLDVVKRQQELLRLTVWGTKNLQTRVTAIEKYLKDQALLNSWGCAFRQVCHTTVPWPNETLTPDWENMTWQQWEKRVNFLDANITALLEEAQIQQERNMYELQKLNSWDVFGNWFDFTSWMAYIRLGLYVVAGLIVLRIVIYIMQMLARLRKGYRPVFSSPPSYTQQIYTTVFYGVPVWKEAQPTLFCASDADITSRDKHNIWATHNCVPLDPNPYEVTLANVSIRFNMEENYMVQEMKEDILSLFQQSFKPCVKLTPFCIKMTCTMTNTTNKTLNSATTTLTPTVNLSSIPNYEVYNCSFNQTTEFRDKKKQIYSLFYREDIVKEDGNNNSYYLHNCNTSVITQECDKSTFEPIPIRYCAPAGFALLKCRDQNFTGKGQCSNVSVVHCTHGIYPMIATALHLNGSLEEEETKAYFVNTSVNTPLLVKFNVSINLTCERTGNNTRGQVQIGPGMTFYNIENVVGDTRKAYCSVNATTWYRNLDWAMAAINTTMRARNETVQQTFQWQRDGDPEVTSFWFNCQGEFFYCNLTNWTNTWTANRTNNTHGTLVAPCRLRQIVNHWGIVSKGVYLPPRRGTVKCHSNITGLIMTAEKDNNNSYTPQFSAVVEDYWKVELARYKVVEIQPLSVAPRPGKR"
  },
  {
    "reference": "MAEQTQLAPAAFHLPDLVAIQRNSFRWFLEEGLIEELESFSPITDYTGKLELHFLGKQYKLKRPKYDVDEAKRRDGTYSVQMYVPTRLINKETGEIKEQEVFIGDLPLMTDRGTFIINGAERVIVNQIVRSPGVYYKSERDKNGRLTHNASLIPNRGAWLKFETDKNGLVWVRIDKTRKLSAQVLLKALGLSDNEIYDKLRHPEYYQKTIDKEGQFSEDEALMELYRKLRPGEPPTVSGGQQLLESRFFDPKRYDLGRVGRYKLNKKLGLNVADTVRTLTSEDILAAIDYLINLELDLGGCEVDDIDHLGNRRVRSVGELLQNQVRVGLNRLERIIRERMTVSDSDSLSPASLVNPKPLVAAIKEFFGSSQLSQFMDQTNPLAELTHKRRLSALGPGGLTRERAGFAVRDIHPSHYGRICPIETPEGPNAGLIGSLATHARVNDYGFIETPFWRVEEGRVRKDLAPVYMTADQEDDLRVAPGDVATDDAGYILGTTIPVRYRQDFTTTTPERVDYVALSPVQIISVATSLIPFLEHDDANRALMGSNMQRQAVPLLRPERPLVGTGLEPQAARDSGMVITSPVDGTISYVDATHIEVTADTGEKYGYALQKYQRSNQDTCLNQRPIVFEGDRVQRGQVIADGSATEKGELALGQNILVAYMPWEGYNYEDAILISERLVYDDVYTSIHIEKFEIEARQTKLGPEEITREIPNVGEDALRQLDENGIIRVGAWVESGDILVGKVTPKGESDQPPEEKLLRAIFGEKARD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must possess DNA binding, ribonucleoside binding to support DNA-templated transcription under certain conditions. The designed protein sequence is ",
    "response#1": "RSPGAYVMEPKDPTKQVLTASLMPHRGSWLEFEVETKGYVSVRIDRKRKLPVTVLLKALGFGGPEEILARFGNSWLIRNTLERDDTTSREDALLEVFRRQRPGEPVNLENAQNLVDGLFFDPKRYDLSEVGRYKVNKKLRLDVPRDVHTLTIEDIEGLLRRLLGIAEEVAEEER",
    "response#2": "RSPGAYVMEPKDPTKQVLTASLMPHRGSWLEFEVETKGYVSVRIDRKRKLPVTVLLKALGFGGPEEILARFGNSWLIRNTLERDDTTSREDALLEVFRRQRPGEPVNLENAQNLVDGLFFDPKRYDLSEVGRYKVNKKLRLDVPRDVHTLTIEDIEGLLRRLLGIAEEVAEEEC",
    "response#3": "METLASRLDACQETLLELYEKDSNKLEDQIKHWAQVRLENVMLFKARECGMTRVGCTTVPALTVSKAKACQAIEVQLALQTLMQSAYSTEAWTLRDTCLEMWEAPPKRCWKKKGQSVLVKFDGSCDRDMIYTGWGHIYVQDINDDTWHKVPGQVDELGLFYVHDGVRVNYVDFGIEALTYGVTGTWEVQVGGRVIR"
  },
  {
    "reference": "MITFVTGNKNKLAEVQAILGDAITSQNIDLDELQGTYNQIITHKLQQAAEIVKGPVMVEDTALEFDALGQELPGPYIKWFLKQLGPEKLPRMLDGFDNRQGSAVCTVGYCDGPESQVHIFQGKTAGTIVDPRGPQTFGWDACFEPLGYTETYAEMAKPEKNKISHRGKAMDKFKQFLSERNSWNSL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be optimized for metal ion binding, nucleotide binding. The designed protein sequence is ",
    "response#1": "AVIAGEKEGAGILTASASLRFGVGLVTMVGEIDTPLPLAIMQDTNLPYNTAAIAFGMGFGKDFENEIVDDILDSKVPIILDADIFHSETILEFLEQNDREIVLTPHPKEFVALWNMTVETPINIALLQANRFDKVREFCEFFPNVTILLKGANMIIAQDNRFFVNPHGSSKLSKGGSGDVLSGLIGGLLAQGKSALDSAIHGSLAMTAGAKAYRGSSYGMLPTDLIEEA",
    "response#2": "LPDAYKSVNEALKHAGLTNRLTVHIKYIDSQDVETKGTDVLKDLDGILVPGGFGYRGVEGKILTAQYARENNIPYLGICLGMQTALIEYARNVAGMKDANSSEFVKDCAYPVIGLITEWQDAEGNVEQRSENSDLGGTMRLGAQQCHLIEGSKARELYGKETIEERHRHRYEVNNTLLPQIEAAGLKVTGLSADRKLVEIIEVPNHPWFVACQFHPEFTSTPRDGHPLFAGFVS",
    "response#3": "FDGASIQRLPARAADAYKNQFGHVLVVGGDSGTGGAALMAAESAARAGAGLVSLATHPQHLVASLTRRPEVMAHPVISGQELEPLLEMPDVIAVGPGLGRAPWGEQLLARAGRADVPLVLDADALNILAGGRVLAGVRRHDWVLTPHVGEAARLLGCGTGDVLADPRAAAIAIQKKFSGVVVLKGAGTLIAHPSGVDLIKGGNPGMASGGMGDLLTGVIAALIGQHMTPLEAARLAVWLHGEAGDRAAADGQRGLLATDLLPHIRKLVK"
  },
  {
    "reference": "MKLSASFAALALLLPFVQAQSPVWGQCGGIGWTGPTTCTAGNVCQEYSAYYSQCIPASQATSVTSVSTAPNPPPTSHTSTSSAPSGASTSTAKLNTLAKAKGKLYFGTATDNGELSDTAYTAILDDNTMFGQITPANSMKWDATEPQQGQFTFSGGDQIANLAKSNGMLLRGHNCVWYNQLPSWVSNGKFTAAQLTSIIQNHCSTLVTHYKGQVYAWDVVNEPFNDDGSWRTDVFYNTLGTSYVQIALEAARAADPDAKLYINEYNIEYAGAKATSLLNLVKTLKAASVPLDGIGFQSHFIVGQVPTGLQSQLTTFAAQGVEVAITELDIRMTLPSTPALLAQQKTDYSNVIKACASVEACVGVTVWDWTDKYSWVPNTFSGQGAACPWDQNFVRKPAYDGIAIGFGN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein contains novel CBM1 domains that confer a unique function or activity.2. The designed protein should have a specific function within the extracellular region. The designed protein sequence is ",
    "response#1": "AHELPESMCLDFKSGVQIQLGFAAEFSNVMIIYTRIVYKPPEIMMCEAYVTDFPFDLDIDPKDANKGTPEETGSYLVSKDLPKHCLYTRLSSLQKLKEHLIFTVCLSYEYPGMEDTIEERQEVNVGKPLIAKLDIHR",
    "response#2": "CPTALPTKNNVRFRLLATTQVGEDVFLVGSIPELGSWDVKKAVPLNADIYADNCHQWYVDIELPTAVAFEYKFIRKRGGEVVWEQDPNRKYTVPQTCGVSGAIKRDTC",
    "response#3": "QSLLDNIGLNGSNAWDTRPGLVIASPSKKDPNYFFTWTRDSALVLKCITDAFAAGNTALQETIHEYISSQARIQLLNTRSGGLSSGGLGEPKYRVDETPYNEDWGRPQADGPALRATALIAYARWLLENDYYDVAKSIVWPVVKNDLSYVSEHWNTTAFDLWEEVNSPSFFTTIVQHRALVEGINIARALDETCPHCESQAPQALCYLQSYWTGTAVRSNYGQGRSGLDVASILGSIHTFDPEGECDDTTFQPCSARALANHKAVTDSFRSIYKINGGIKQGEAVAVGRYPEDVYFNGNPWYLATYAAAEQLYDAMYQWNKIGKITVTDVSMPFFKDIYPEVQTGTHESSSPEFGNIIAAVKAYAEGYIEVAKKYTPCTGMLSEQFSRDNGTPLSVADLTWSYASYLTVYG"
  },
  {
    "reference": "MNGAKRRKVAQDTPRNTKPVAQEKPARAEPKPSSDEESEEESATLEEPSAEETAVDAPKKTFKDLGVNDALCEACEKLNYKYPTPIQEKSIPVALQGRDIIGLAETGSGKTAAFALPVLQALLDKPQPLFGLVLAPTRELATQIGQAFEALGSLISLRCAVIVGGLDMVPQAIALGKKPHIVVATPGRLVDHLEKTKGFSLRTLKYLIMDEADRLLDMDFGPSIDKILKFVPRERRTYLFSATISSKIESLQRASLRDPVKVSISSNKYQTVSTLLQNYLFIPHPQKDVHLIYLINEHAGQSTIVFTRTVWETQRVSILLRTLGFGAIPLHGQLSQSSRLGALNKFRSGTRDILVATDVAARGLDIPSVDVVLNYDLPQDSKTYIHRVGRTARAGKSGVAISLVTQYDLEIYLRIEAALGKKLAEYPTEKEEVMAFQSRVEEAQRIARIEMKSFTEERGKKGSTLKGGRGKKGGKRGRDDMDREEG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.2. The protein design should include a DEAD box, Q motif motif to ensure proper function.3. The protein should be optimized for nucleus-specific folding and stability, while maintaining ATP hydrolysis activity, RNA binding, RNA helicase activity. The designed protein sequence is ",
    "response#1": "IPIALQCRDLIGISKTGSGKTAAFVLPMLSYIEPLPPLNEVTKTEGPYALILAPTRELATQIQAEVIKFATRMGFTVVCLIGNKRTIEEDAFALRNGAEIIVATPGRLVDCLERHLLVLSQCSYVVLDEADRMVDGGFEDSIHKILAALPPSNGKPDDRDAEDPNIMSKFLTPNLRYRQTVMYSATMPPSVERIAKNYLKHPAMVN",
    "response#2": "FTSPPLDELLLENIKLARFTKPTPVQKYSVPIVAKGRDLMACAQTGSGKTGGFLFPVLSQSFSNGPASTPDESGYYMRKAYPTAVVLAPTRELATQIFDEAKKFTYRSWVKPCVVYGGADIRQQIRELERGCDLIVATPGRLNDLLERGKISLCSVKYLVLDEADRMLDMGFEPQIRHIVEGCDMPTVENRQTLMFSATFPTDIQHLAADFLKDYIFLSVGRVE",
    "response#3": "IPEIMAGRDVIGKASTGSGKTLAFGIPIIESYLASKSKSKDVKDKTPLALIIAPTRELAHQITAHLTALCAKGAFEAPLIASVTGGLAVQKQRRQLEKADIIVGTPGRLWEVISTGHGLLEKVKQIRFLVVDEADRLLSQGNYKELGEILKILEKDAPAEGEAEAEETTEVERQTLVFSATFQKGLQQKLAGKAKGGSDE"
  },
  {
    "reference": "MPFNLKNRHLLSLVNHSPREIKYLLDLARDLKRAKYAGTEQPRLKGKNIALIFEKTSTRTRCSFEIAAYDQGANVTYIDPTSSQIGHKESMKDTARVLGRLYDAIEYRGYKQETVEELAKFSGVPVFNGLTDEFHPTQMLADVLTMIEHSTKPLNEIKYVYIGDARNNMGNSLLLIGAKLGMDVRICGPKSLLPEENFVSICEEISKETGARLTVTDDIDLAVKDADFVHTDVWVSMGEPIEAWGERINLLMPYQVNTDLMKRTGNPNVKFMHCLPAFHNCETKVGREIAAAYPNLANGIEVTEDVFESPMNIAFEQAENRMHTIKAVMVASLA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be able to bind carbamoyl phosphate ligand in a variety of conditions2. The protein should be optimized for stability and expression in cytoplasm where it will participate in arginine biosynthetic process.3. The protein should be optimized for activity in L-arginine biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "FSTDANLLHTSSEGKVLEDPWEEVPDYVYSRTVNPEDAPDQPEIITIDFERGDGVALNGEGMSPATLLTALNELGRKHGIGRLDLVENRFVGMKSRGMYETPGGAIYHLAHRGIEQITLDRGAAHLKDELAPRYAELIYNGFWFSPEREMLQAAIDHSQEKVTGTVRLKLYKGGVHVIGRKSPYTLYSEKVVTFEDDAGAYDQRDAAGFIKLNALRLG",
    "response#2": "YSTDANIWGATHEAKKLEHLDTGIEIVDPIMGVPFWDDSVEIATEDVTLSFVQGRPVAINGKEFDSPVDLVHEANAVGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLFIAYERLVNAIHNEDTIASYHNEGRRLGRLMYEGRWFDPQSLMLRESLQRWVGSAVTGEVTLRLRRGEDYSILDTTGPAFSYHPDKLSMERTEY",
    "response#3": "FSTDANLLHTSSEGKVLEDPWEEVPDYVYSRTVNPEDAPDQPEIITIDFERGDGVALNGEGMSPATLLTALNELGRKHGIGRLDLVENRFVGMKSRGMYETPGGAIYHLAHRGIEQITLDRGAAHLKDELAPRYAELIYNGFWFSPEREMLQAAIDHSQEKVTGTVRLKLYKGGVHVIGRKSPYTLYSEKVVTFEDDAGAYDQRDAAGFIKLNALRLR"
  },
  {
    "reference": "MTSKKKILVIGGGFSGIGSLKSLKEEGFDPICYEKTSDLGGTWHYREETPFGVPSIMPTTVINHSKEMGTLSDFIPNKSLPNYLRHHELLDLFKEIANKFDCVKHIQYNREVIKVKRSADYDETGRWDVKVRNTENGEISDEVFDGVMVSVGQFTYPKMAEFPGMEKFKGPIIHTHSLKGVHKFLNKKVLVIGIGCSGLDAAAEISNVASQVYLSSRNGTWLLPRIGPFGLPFDYVILRRYGTLMQKFFGWKFASWYLETFQLNKKFNHNLYNLCPEYPALAKDPAINDLLPAKLVSGSVKLRKNVKCFTEKGVIFENETHVTEVDAVIMATGYKWQFPFLEDGIVVNEDGRINLYKCMYPPHLKHPTLVVIGFILPFGPGFPCGEMQCRWATQIFSGNCKLPSEDEMMADIIKRHEENVKRYAPSDKVTVKVDFIQYLDEIASQFGAKPNLWKILFTDPKLWRAIVFGPSLPYQYRLQGPHKWHGARDAILTSYSRVRYPLSGEPKKKSTKPKFTFGFYFKYIVTFLLMAFWLTQSETSVKYYLLALILPYFMTWKGFYKKYFFSLLMLP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein with N,N-dimethylaniline monooxygenase activity is needed to enhance lipid metabolic process in a specific context.2. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "MXKXGXVX",
    "response#2": "LPLL",
    "response#3": "SWVG"
  },
  {
    "reference": "MSKQFSHTTNDRRSSIIYSTSVGKAGLFTPADYIPQESEENLIEGEEQEGSEEEPSYTGNDDETEREGEYHSLLDANNSRTLQQEAWQQGYDSHDRKRLLDEERDLLIDNKLLSQHGNGGGDIESHGHGQAIGPDEEERPAEIANTWESAIESGQKISTTFKRETQVITMNALPLIFTFILQNSLSLASIFSVSHLGTKELGGVTLGSMTANITGLAAIQGLCTCLDTLCAQAYGAKNYHLVGVLVQRCAVITILAFLPMMYVWFVWSEKILALMIPERELCALAANYLRVTAFGVPGFILFECGKRFLQCQGIFHASTIVLFVCAPLNALMNYLLVWNDKIGIGYLGAPLSVVINYWLMTLGLLIYAMTTKHKERPLKCWNGIIPKEQAFKNWRKMINLAIPGVVMVEAEFLGFEVLTIFASHLGTDALGAQSIVATIASLAYQVPFSISVSTSTRVANFIGASLYDSCMITCRVSLLLSFVCSSMNMFVICRYKEQIASLFSTESAVVKMVVDTLPLLAFMQLFDAFNASTAGCLRGQGRQKNRWVHQPSRILLPRCAHGICVSIPVSSGCRRLMVGYN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Prioritize Polar residues, Acidic residues compositional bias within the protein sequence to achieve desired characteristics.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. The designed protein should be involved in xenobiotic detoxification by transmembrane export across the plasma membrane, S-adenosylmethionine biosynthetic process by localizing to membrane and exhibiting antiporter activity, xenobiotic transmembrane transporter activity, transmembrane transporter activity.4. The Extracellular, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.5. The protein must exhibit the following characteristics: Probable transporter involved in ethionine resistance; Overproduction leads to accumulation of S-adenosylmethionine.. The designed protein sequence is ",
    "response#1": "LSFHNISYQETVQSGFPLRKKAYVIERLSNISGIMKPGLNAIMGPQDGSRSLLLDVLAARRDPRGLSGDILINGKPRPANFKCTSGYVPQNDVVLGTVTVRDNLEFSAALRLPVTITRDEKRRRINEVLELLHLNKEQNIKPRSKELRKRTSIAMELVTEHPILFLDDPTTGLDLRTTTDVILVLRRMSKKGRTIIFSINQPQYSIFKFFDSLTLVASGKVMFHGPAQDALEYFRSAGYNYEEETKEKATEFFLELVRG",
    "response#2": "QWQLLHLNGTFPNTNEPDTEPCVDGWVYDRSSFLSTIVTEWDLVCESQSLKSMVQSLFMAGSLLGGLIYGHLSDRVGRKIICKLCFLQLAISNTCAAFAPTFLVYCILRFLAGFSTMTILGNTFILSLEWTLPRSRSMTIMVLLCSYSVGQMLLGGLAFAIQDWHILQLTVSTPIIVLFLSSWKMVESARWLIINNQLDEGLKELRRVAHINGKKNTEETLTTELVRSTMKKELDAVRIKTSIFSLFRAPKLRMRVFGLCFVRFAITVPFYGLILNLQHLGSNVSLFQILCGAVTFTARCVSLLTLNHMGRRISQILFTFPVGLFILVNTFLPQEMQILRVVLATLGIGSVSAASNSASVHHNELVPTILRSTVAGINAVSGRTGAALAPLLMTLMAYSPHLPWISYGVFPILAVPVILLLPETKNSEFYQISND",
    "response#3": "QWQLLHLNGTFPNTNEPDTEPCVDGWVYDRSSFLSTIVTEWDLVCESQSLKSMVQSLFMAGSLLGGLIYGHLSDRVGRKIICKLCFLQLAISNTCAAFAPTFLVYCILRFLAGFSTMTILGNTFILSLEWTLPRSRSMTIMVLLCSYSVGQMLLGGLAFAIQDWHILQLTVSTPIIVLFLSSWKMVESARWLIINNQLDEGLKELRRVAHINGKKNTEETLTTELVRSTMKKELDAVRIKTSIFSLFRAPKLRMRVFGLCFVRFAITVPFYGLILNLQHLGSNVSLFQILCGAVTFTARCVSLLTLNHMGRRISQILFTFPVGLFILVNTFLPQEMQILRVVLATLGIGSVSAASNSASVHHNELVPTILRSTVAGINAVSGRTGAALAPLLMTLMAYSPHLPWISYGVFPILAVPVILLLPETKGRTLEQITAHFEGR"
  },
  {
    "reference": "MKAPATIVILIMSLISVLWATADTEDGNLLFPIEDFIRKFDEYPVQPKERSCKLTFWRCKKDKECCGWNICTGLCIPPGKK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Intrathorax injection into crickets causes death.; Intracranial injection into mice causes lacrimation, slow breathing and death.2. The protein should be optimized for extracellular region-specific folding and stability, while maintaining toxin activity. The designed protein sequence is ",
    "response#1": "RDGYVVKNGTNCKYSCEIGSEYEYCGPLCKRKNAKTGYCYAFACWCIDVPDDVKLYGDDGTYCR",
    "response#2": "ADGYIRGGDGCKVSCVINHVFCDNECKAAGGSYGYCWAWGLACWCEGLPADREWDYETNTCS",
    "response#3": "RDGYVVKNGTNCKYSCEIGSEYEYCGPLCKRKNAKTGYCYAFACWCIDVPDDVKLYGDDGTYCK"
  },
  {
    "reference": "MNYPGHGSPRSSERNGGRGGDGAAWELGSDTEPAFGGSVCRFDHLPVGEPGDDEVPLALLRGEPGLHLAPGAEDHNHHLALDPCLSDDNYDFSSAESGSSLRYYSEGESGGGGSSSSLHPPQQPLVPSNSGGGGAAGGGPGERKRTRPGGAAARHRYEVVTELGPEEVRWFYKEDKKTWKPFIGYDSLRIELAFRTLLQATGARARAQDPDGDHVCGPASPAGPASSSVEDEDEDRVCGFCPRIAGHGREMEELVNIERVCVRGGLYEVDVTQGECYPVYWNQSDKIPVMRGQWFIDGTWQPLEEEESNLIEQEHLSRFRGQQMQESFDIEVSKPIDGKDAIHSFKLSRNHVDWHSVDEVYLYSDATTSKIARTVTQKLGFSKASSSGTRLHRGYVEEATLEDKPSQTTHIVFVVHGIGQKMDQGRIIKNTAMMREAARKIEERHFSNHATHVEFLPVEWRSKLTLDGDTVDSITPDKVRGLRDMLNSSAMDIMYYTSPLYRDELVKGLQQELNRLYSLFCSRNPNFEEKGGKVSIVSHSLGCVITYDIMTGWNPVRLYEQLLQKEEELPDERWMSYEERHLLDELYITKRRLREIEERLHGLKASSMTQTPALKFKVENFFCMGSPLAVFLALRGIRPGNTGSQDHILPREICNRLLNIFHPTDPVAYRLEPLILKHYSNISPVQIHWYNTSNPLPYEYMKPSFLHPAKDPTSISENEGISTIPSPVTSPVLSRRHYGESITNIGKASILGAASIGKGLGGMLFSRF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.2. The designed protein should contain one or more DDHD domains that are essential for its function.3. The designed protein should have phospholipase activity, phospholipase A1 activity, metal ion binding within cytoplasm to regulate lipid metabolic process.4. A key design requirement for the protein is the optimization of the active site to achieve high catalytic efficiency for 1-acyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-   phosphoethanolamine + H2O = 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-   glycero-3-phosphoethanolamine + a fatty acid + H(+).5. The protein must exhibit the following characteristics: May play a regulatory role in spermatogenesis or sperm function.; Phospholipase A1 (PLA1) that hydrolyzes ester bonds at the sn-1 position of glycerophospholipids producing a free fatty acid and a lysophospholipid; Changes in these lipids extend to downstream metabolic products like PI phosphates PIP and PIP2, which play fundamental roles in cell biology ; These dynamic changes may be due to PA hydrolysis at the mitochondrial surface ; Involved in the regulation of the endogenous content of polyunsaturated PI and PS lipids in the nervous system. The designed protein sequence is ",
    "response#1": "MSKVLLHGTLHVTVYEVDKLREGGGPNVFGKLMANIQETVGFGEGTPKIYATIDLEKSRVGRTRMIENEPQNPRWYESFHIYCAHHASNIIFTVKDDNPIGATLLGRAYMPVRELLDGDEVDKWFELMDQKGQSRGSILLTLKVIEAPPFATSSGSLRVELLHGNLDIWVKEAKHLPNMDGFHNRLGGMLSGLGRKKVEGEKSSKITSDPYVTVSISGAVIGRTFVISNSENPVWMQHFDVPVAHSAAEVHFVVKDSDIIGSQIMGAVGIPTEQLCSGNRIEGLMSKVLLHGTLHVTVYEVDKLREGGGPNVFGKLMANIQETVGFGEGTPKIYATIDLEKSRVGRTRMIENEPQNPRWYESFHIYCAHHASNIIFTVKDDNPIGATLLGRAYMPVRELLDGDEVDKWFELMDQKGQSRGSILVTLKVVEAPPFATSSGSLRVELLHGNLDIWVKEAKHLPNMDGFHNRLGGMLSGLGRKKVEGEKSSKITSDPYVTVSISGAVIGRTFVISNSENPVWMQHFDVPVAHSAAEVHFVVKDSDIIGSQIMGAVGIPTEQLCSGNRIEGLHELMDQKGQSRGSILVTLKVVEAPPFATSSGSLRVELLHGNLDIWVKEAKHLPNMDGFHNRLGGMLSGLGRKKVEGEKSSKITSDPYVTVSISGAVIGRTFVISNSENPVWMQHFDVPVAHSAAEVHFVVKDSDIIGSQIMGAVGIPTEQLCSGNRIEGL",
    "response#2": "MSKVLLHGTLHVTVYEVDKLREGGGPNVFGKLMANIQETVGFGEGTPKIYATIDLEKSRVGRTRMIENEPQNPRWYESFHIYCAHHASNIIFTVKDDNPIGATLLGRAYMPVRELLDGDEVDKWYTLVKQATNKDPFVFVHGFTGFVGEVAAKGENHWGGTKANLRNHLRKAGYETYEASVSALASNHERAVELYYYLKGGRVDYGAAHSEKYGHERYGKTYEGVLKDWKPGHPVHFIGHSMGGQTIRLLEHYLRFGDKAEIAYQQQHGGIISELFKGGQDNMVTSITTIATPHNGTHASDDIGNTPTIRNILYSFAQMSSHLGTIDFGMDHWGFKRKDGESLTDYNKRIAESKIWDSEDTGLYDLTREGAEKINQKTELNPNIYYKTYTGVATHETQLGKHIADLGMEFTKILTGNYIGSVDDILWRPNDGLVSEISSQHPSDEKNISVDENSELHKGTWQVMPTMKGWDHSDFIGNDALDTKHSAIELTNFYHSISDYLMRIEKAESTGTLHVTVYEVDKLREGGGPNVFGKLMANIQETVGFGEGTPKIYATIDLEKSRVGRTRMIENEPQNPRWYESFHIYCAHHASNIIFTVKDDNPIGATLLGRAYMPVRELLDGDEVDKP",
    "response#3": "MVSQGAFAGMSRRAFLAKAAGAGAAAVLTDWAAPVIEKEKLPPIKPRQGPVPEPPQNKDPFVFVHGFTGFVGEVAAKGENHWGGTKANLRNHLRKAGYETYEASVSALASNHERAVELYYYLKGGRVDYGAAHSEKYGHERYGKTYEGVLKDWKPGHPVHFIGHSMGGQTIRLLEHYLRFGDKAEIAYQQQHGGIISELFKGGQDNMVTSITTIATPHNGTHASDDIGNTPTIRNILYSFAQMSSHLGTIDFGMDHWGFKRKDGESLTDYNKRIAESKIWDSEDTGLYDLTREGAEKINQKTELNPNIYYKTYTGVATHETQLGKHIADLGMEFTKILTGNYIGSVDDILWRPNDGLVSEISSQHPSDEKNISVDENSELHKGTWQVMPTMKGWDHSDFIGNDALDTKHSAIELTNFYHSISDYLMRIEKAESTS"
  },
  {
    "reference": "MSQRQTPSQADISDENIDFGFRTVRRAEKAGLVRDVFDSVATRYDIMNDLMSGGVHRLWKAAMIDWMAPQPYQHLVDLAGGTGDISLRFLKAGGGTASVTDINESMLAAGRRRRDLGKLADRLSWCVGNAEALPFETASADLVTIAFGLRNVTNREAALAEAHRILRPGGRFLCLEFSQVSNEMLARAYDSWSFNVLPRLGQLVAGDADSYRYLVESIRTFPTPEVLADMFAGAGFAQVRVRRMSAGIACIHSGWKLD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2).2. The designed protein should have S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity, S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity, demethylmenaquinone methyltransferase activity, 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity to facilitate ubiquinone biosynthetic process, menaquinone biosynthetic process.3. A protein that is capable of catalyzing reactions within the menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2, menaquinone biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MSRAMPLRDLLAGVPLDGARDGVYDTANRVMSMGVDISAYDFLNHFLSAGRDRMEARVLKPGGTMVCLEFLPAGFGALALEDVSPALAMARLAARFYDNPAQKLRLVGA",
    "response#2": "MFATIEEAIADIKAGKMIIVVDDEDRENEGDLVLAAEMVTPEAINFMAKFGRGLICMPMTGERLDELEIPLMVSNQTDSFQTAFTVSIDAKETTTTGISAFERAATIKALIDPNTKPEDLARPGHVFPLRAKKGGVLVRAGQTEGSVDLARLAGLKPAGVICEIMNDDGTMARMPDLEKFAEKHNLKIVTIADLIKYRLKKEKLVKRECVELETQTVPVKMGEFEIRTMETKHEHLHTEVPQEDQKNLLYLKVKQEKMGHYLDCNSNYLHYKKKKLETVSHDV",
    "response#3": "MPATLNQAIEWLKQQAQGPRIAITQGSSQRGIDSLLAAVRDIDCWPADQLIGIATDGYVAVPALLTNKPLKAIIHMPHIRAENAAVPQAQCSYQPEQVQQLASQLLQQQPSRVLNLSVGGEAAAQWQAVADWCQQQPLPIAFNAPGSLDGQALQQAGAQLLLKGNVTGVDNLVQAFQPPALQTEPAA"
  },
  {
    "reference": "MATQFPLSSEFERLTVYGPTRATVSGTPLDAEEVRKIHAFWRACNYLALGMIYLRGNPLLREPLKPEHIKHRLLGHWGSSPNLAFVYTHLNRAIRKHDLDMIFMAGPGHGAPGVLGPLYLEGSYSEIYPDKDLSEEGLLNFFKQFSFPGGIGSHCTPETPGSIHEGGELGYVLSHACGAAFDNPDLIVAAVVGDGEAETGPLATSWHINKFLNPIRDGAVLPILNLNGYKINNPTLLARISHEELENLLRGYGYTPYFVEGSEPESMHQAMAATVDRSIEDIRAAQTEARASGIARRPRWPMIVLRSPKGWTAPRQIDGHNVEGFWRAHQVPVADVAKNPEHLKLLEGWMRSYKPEELFDAEGCPVAEIREMAPAGLRRMGLNPHANGGHLKKALRIPNFRNYGIEVAKPGQIEAPNTQPLGVFLRDVMKENAHNFRLFGPDENTSNKLDAVYAAAKKFWIAEYFPEDQDGGELAPDGRVMEMLSEHTLEGMLEGYLLTGRHGFLSTYEAFVHVIDSMFNQHAKWLSICNQLSWRQDVASLNLLITSTVWRQDHNGFTHQDPGFLDVVVNKSADVTRIYLPPDVNSLLSVADHCLRSQNYINVIVSDKQLHLQFMDMDAAIAHCTEGLGIWEWASNDEGQEPDVVMACAGDIPTLEALAATALLREEFPELKIRFINVVDLFKLQPESEHPHGLSDKDFDSLFTRDKPVIFNFHGYPWLIHRLAYRRTNHANMHVRGYKEKGNINTPLELAINNQIDRFSLAIDVIDR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have aldehyde-lyase activity to facilitate carbohydrate metabolic process. The designed protein sequence is ",
    "response#1": "SDDLMQKMDAYWRAANYLSVGQIYLQDNPLLEQPLRIEHIKPRLLGHWGTTPGLNLLYVHLNRLISAHDLDMIYIIGPGHGGPGLVANSYLEGSYTERYPAIERNRNGLQRLFRQFSWPNGVPSHVSPETPGSIHEGGELGYSLAHAYGAAFDNPDLIVACIVGDGEAETGALATSWHSNKFLNPARDGAVLPILHLNGFKIANPTILARIGRQELTDLMRGYGYEPIVVEGDDPKLVHHTLAAALERALADIRAIQAAARHQGVTVRPRWPMIILRTPKGWTGPKQVDGKQIEGTWRAHQVPIASFKDPTHVQLLETWLRSYRPEELFDASGKFRDDLAALAPTGHRRMSANPHANGGR",
    "response#2": "KQPALQYLDMDAAIKHCTKGIGIWDWASNDQGVEPDVVMACCGDIPTLETLAAVDILRQNFPDLKIRVINVVNLMKLQPESEHPHGLSDKDFDSIFTPDKPVVFAFHGYPWLIHRLTYRRNNHNNIHVRGYKEEGTTTTPFDMVVMNDLDRFNLADDVIDRVPRLRYTAAHVKQMLHC",
    "response#3": "KQPEWQWLDIDSAVRHCSAGAGIWHWASNGEDDPDVVMACAGDVPTLETLAAVMLLREYVPDIRVRVVNVVDLMVLQPSSEHPHGLDDKRFDEIFTVDKPVVFAFHGYPWLIHRLTYRRRNHFNIHVRGYKEEGSTTTPFDMVVLNDLDRYRLALDAIRRIPRLAGEVEAATARYWATMQRHKLYC"
  },
  {
    "reference": "MLTHDLTKNLGRPSFLPKELTISDSQVQRLKTPCFEEIIRSFLNKRKPKQNWQLSLEIEEKLSNPTILDIFKTIRTTEETIISDESSRDFFIYSLLKIDCCIKGNLFSYYSEMDRTNIKDIIIGKGLKNNNNNIILGNSIFFKKIVEDFLTMAFERFSWYCENKSTDLLFFICVMSESNERLKEFFNKFQDYHHHHNQSKTAEHYKISKSLNSLPFSISNGVISNGVNPTIKIQWFLKFVELNLKNPIVYQLFDYTIINYPFISNLNILVGPILLNIFNNDNIKIYEDNNNNNNNNNNNNNNNNNNNSTIVDKFNLTLELKSKLAFSVYKRKLAICAVTLPILVLHLQKDPIGFLKYYDEFIKSSPESKSLFNHSISLHLSNDTKKMFNLLPYRNRIDLGWESFIVNKLTTADKINILSTYDETYQDVKAIIICSLNVESYALLSLESKLSDQFLINVFNQQCRLPNNYNFSGVITSFSTIEREYVSRLKNSIEQEAIKKKQLIVKQIEMNSEIYENYFRIVNVMVEALLNQNPSLNSIIECVGTDLGGSLEQDNQYMDTLPNLVLFSHLLRRIATSNLNSSYLLKKDSKGNNRLNLDLSNLAMFYNSKNSFWQEIRGPYDSFLREFIKNLLAKYVEIKSDFKYLDYHYFESLTIILLEMKRRYNCIPSDYRVSNKPVKIVEFKDFIKKFEEKESQYNDLLLVKEYLERKFSFNFSSQFDKNWKTSKRLKLLRQLIKSTKSLYGHYIQDDESIKFLLHFAIVNKTLFF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should emphasize Acidic residues, Polar residues, Basic and acidic residues compositional bias.2. The designed protein must be able to perform ATP hydrolysis activity under specific conditions. The designed protein sequence is ",
    "response#1": "EVLGKGAMKTVYKAFDQVLGMEVAWNQVKLNEVFRSPEPLQRLYSEVHLLKNLNHESIIRYCTSWIDVNRRTFNFITELFTSGTLREYRRKYQKVDIRAIKSWARQILNGLAYLHGHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAILRGSQNAHSVIGTPEFMAPELYEEDYNELVDIYSFGMCVLEMLTGEYPYSECTNPAQIYKKVTSGKLPDSFHLIQHTEAQRFVGKCLETVSRRLPAKELLADPFL",
    "response#2": "GRFREVLGKGAMKTVYKAFDQVLGMEVAWNQVKLNEVFRSPEPLQRLYSEVHLLKNLNHESIIRYCTSWIDVNRRTFNFITELFTSGTLREYRRKYQKVDIRAIKSWARQILNGLAYLHGHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAILRGSQNAHSVIGTPEFMAPELYEEDYNELVDIYSFGMCVLEMLTGEYPYSECTNPAQIYKKVTSGKLPDSFHLIQHTEAQRFVGKCLETVSRRLPAKELLADPFF",
    "response#3": "NGEMRDYQVRGLNWLASLQHNKINGILADEMGLGKTLQTISMIGYMKHYKNKASPHLVIVPKSTLQNWANEFKKWCPSINAVVLIGDEAARNQVLRDVILPQKFDVCCTTYEMMLKVKTQLKKLNWRYIIIDEAHRIKNEKSKLSETVRELNSENRLLITGTPLQNNLHELWALLNFLLPDIFTSSDDFDSWFSNDAMSGNTDLVQRLHKVLQPFLLRRII"
  },
  {
    "reference": "MKLETPRFDSARILVLGDVMLDRYWHGTTSRISPEAPVPVVKVEELEESPGGAANVALNIAALGAACSLSGLIGRDREGEILTSVLEAAEIRCDFLPVADKPTITKLRVLSRHQQLIRLDFEQEFAQADAQGLAGKLQALIKDVDLLIFSDYQKGALSDISKLIAYAREAGVPVLVDPKGNDFSKYRGATLLTPNLHEFEAIVGSCSTEKELVDKGLELISQLELQALLVTRGEHGMSLIRPGETELHFPARSRDVFDVTGAGDTVISVLGASLAAGKSLPEAVALANIAAGIAVSRVGTVAVSGPELRREVQQDSDSDRGVMSREQLLLAVADAKANGERLVFTNGCFDIIHAGHVGYLQEAKQQGDRLIVAINDDASVTRLKGEGRPINSLDRRMAVLAGLEAVDWVVGFDEDTPIPLLELLKPDVLIKGGDYDIDEVVGADIVQQAGGEVKVLAFLDNCSTSGIVEKIRKEK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should consider the endogenous proteins and enzymes present in the ADP-L-glycero-beta-D-manno- heptose biosynthesis, Nucleotide-sugar biosynthesis pathway to avoid interference with native metabolic processes.2. The protein should be able to bind ATP ligand in a variety of conditions3. The designed protein is the optimization of the active site to achieve high catalytic efficiency for ATP + D-glycero-beta-D-manno-heptose 7-phosphate = ADP + D-   glycero-beta-D-manno-heptose 1,7-bisphosphate + H(+).4. The protein must have high specificity towards heptose 7-phosphate kinase activity. The designed protein sequence is ",
    "response#1": "MPRFDQSRVLVVGDLMLDRYWHGNTSRVSPEAPVPVVDVKQIEDRPGGAGNVALNISALGSASVLAGLVGRDEAAGVLQQRLEAANILCQFQVSETHPTITKLRVISRHQQLLRLDFENPFAPEDAQALSQTALTALKDAKALVISDYAKGALQDPQPLIQAARAQGIAILVDPKGTDFRKYRGATLLTPNLHEFEAIVGKTTSEQDLVEKAQSMIHELELQALLVTRGEHGMSLIRPGEPEFHLPARAREVFDVTGAGDTVISVLAAALGAGDDLPNAVALANLAAGIVVGKLGTAAISAPELRRALHGLPHQAAGRARLLTADELTVALTNGCFDLLHAGHVQYLEQAAREGDCLIVALNSDASVRRLRKGPDRPLFDQEHRARMLAALEAVDYVVIFEEDTPHAVLRALKPDVLVKGGTYRDEEIVGREVVLEYGGRVKALGVTPGLSTTEIVRRV",
    "response#2": "MPRFDQSRVLVVGDLMLDRYWHGNTSRVSPEAPVPVVDVKQIEDRPGGAGNVALNISALGSASVLAGLVGRDEAAGVLQQRLEAANILCQFQVSETHPTITKLRVISRHQQLLRLDFENPFAPEDAQALSQTALTALKDAKALVISDYAKGALQDPQPLIQAARAQGIAILVDPKGTDFRKYRGATLLTPNLHEFEAIVGKTTSEQDLVEKAQSMIHELELQALLVTRGEHGMSLIRPGEPEFHLPARAREVFDVTGAGDTVISVLAAALGAGDDLPNAVALANLAAGIVVGKLGTAAISAPELRRALHHGHGQRDFGIVSEEQLALAVSARRKAGQKIVLTNGCFDLLHAGHVQYLEQAAREGDCLIVALNSDASVRRLRKGPDRPLFDQEHRARMLAALEAVDYVVIFEEDTPHAVLRALKPDVLVKGGTYRDEEIVGREVVLEYGGRVKALGVTPGLSTTEIVRRLAREHSRGKKVVFTNGCFDLLHVGHVKYLQKARGLGDILVVGLNTDASVRRLKGEKRPLIEETERAHILAALDCIDYVVLFDEDTPLKVIETLGPDILVKGGDYSIEGVVGREVVEARGGRVELIQFVDGRSTSRIIEKIRA",
    "response#3": "MPRFDQSRVLVVGDLMLDRYWHGNTSRVSPEAPVPVVDVKQIEDRPGGAGNVALNISALGSASVLAGLVGRDEAAGVLQQRLEAANILCQFQVSETHPTITKLRVISRHQQLLRLDFENPFAPEDAQALSQTALTALKDAKALVISDYAKGALQDPQPLIQAARAQGIAILVDPKGTDFRKYRGATLLTPNLHEFEAIVGKTTSEQDLVEKAQSMIHELELQALLVTRGEHGMSLIRPGEPEFHLPARAREVFDVTGAGDTVISVLAAALGAGDDLPNAVALANLAAGIVVGKLGTAAISAPELRRAIDGGAAAHVLCVGDVMLDRFVYGEVERISPEAPIPVMHIAREVVTVGGAGNVARNLSALGTATRLFGVVGDDPAGREAAEVLSFESSVSLSLTADGGRPTTVKTRFIAGGQQLLRTDEEQLSALSDGLINKLCGFLTAAVSDGECGALVLSDYGKGLVTDMMIAAAMEAATAAGVPVIVDPKGNDFTRYRGADVITPNRRELSAASRMPADADDEVTAAAHHLITSCGLGAVIATRGAAGMTVVTETDVVHLPALAREVFDVSGAGDTVVATLAAGLAAGMKLADAAQLANVAAGLVVAKVGTAVVRADEVAEWRAAGETIVFTNGCFDLLHVGHVALLEECRGFGSKVVLGLNTDVSVQRLKGPSRPVVGERERAKVMSALAAVDAVVLFDEETPLALIEALQPDVLVKGGDYTVETVVGHEVVLAAGGRVEIVPIVQGFSTTNIMRKLQYGRSAG"
  },
  {
    "reference": "MNGAYAASFLPVILVPLAGVVFPALAMGLLFNYIESDA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that May help in the organization of the PsaL subunit.2. The designed protein localizes to the photosystem I. The designed protein sequence is ",
    "response#1": "PQALNNIAVIYHYRGEQALAAGNIPDSETLFEKAAEYWKEAIRLAPLF",
    "response#2": "MPIGVPKVSAPGQ",
    "response#3": "PQALNNIAVIYHYRGEQALAAGNIPDSETLFEKAAEYWKEAIRLAPLV"
  },
  {
    "reference": "MAAKEIGKLVFVTGNKNKLSEVRAILSGTIEVESHKVDLPELQGTTQDIAKQKCKQAAEILNGPCITEDTCLCFDAMNGLPGPYIKWFLEELGHDGLNKMLVGFNDYSAYALCTFAYCRGPGYEPVIFEGRTQGKIVPARGPGDFGWDPIFQPDGFAETYAEMSKEVKNTISHRYRALEKLKEYLQTSA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that The enzyme does not distinguish between the deoxy- and ribose forms; Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives; Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.2. The designed protein should have a binding site that is specific to the target ITP, Mg(2+) ligand.3. The protein should have a fast turnover rate for dITP + H2O = dIMP + diphosphate + H(+).4. A protein that contributes to deoxyribonucleoside triphosphate catabolic process, nucleotide metabolic process by functioning in nucleus with nucleotide binding, metal ion binding, dITP diphosphatase activity.5. The protein must be engineered to have a cooperative binding mechanism with Mg(2+), Mn(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "MQILLATNNQNKITEFRAELGGSVEVLGPEDIGCFEDVPETHNTFEGNALQKAHYLAKHLDQPVLADDSGLCVDALQGMPGVLSARYSGRHGDDEANNELLLAQLSDVPAERRTAHFHCTLVLMRHADDPQPLVASGQWHGRILTELHGQGGFGYDPLFYLAELGASAGELPPERKNTLSHRGQALRQLLPQLQQLLSQ",
    "response#2": "MTTALVTGANRGLGRAFVEELASRGWDVVATGRDPEALEAVAAQHPGVRTVPLDLATADGPDALAETVGDLDVLVNNAGVYHDAASARGEMTAADFNANLEVNLTAPVRLVRALMPALRSGAGRVINISGGGMNTPYPGLSAYSAAKAGLSMMTWALAAEVTPSGVAVNAVAPGFIATDLTAGIRTAQPIEGGWTPLRTVADVVRWAAEQPRNATGETIRVRGGEITG",
    "response#3": "MKIVLATRNRNKAREICDILSNLCPGIEVLNLADFPGVPDIKETGVTFAENSMLKASAVASATGLITLADDSGLEVDALGGAPGIYSARFAGENATYQDNLNKVLSGLADIPQEKRGARFVCAIALVVPGNNLWAVEGRLEGRILTEPRGHNGFGYDPVFYLPEYGCTSAELAPQEKNQISHRGKALALMLAVLQQKIR"
  },
  {
    "reference": "MSQQEEPHHADKDPDLRSTEVTQGVEKAPHRAMFRAMGYDDEDLAAPMVGVANPAADITPCNVHLDDVADSAYEAVDAADGMPIEFGTITISDAISMGTEGMKASLTSREVIADSVELVSFGERMDGLVTIGGCDKNMPGMMMAMIRTDLPSVFLYGGSIMPGEHEGREITVQNVFEGVGAVAQGEMSEDELDEMERHACPGAGSCGGMFTANTMASVAEALGLAPLGAAGPPAEHEDRYEVAERAGELAVDAIENDRKPSDILTKKSFENAIALQVAVGGSTNAVLHLLAMAAEAGVDLSIEEFDEISQRTPKIADLQPGGTRVMNDLYEIGGVPVVLRRLLDAGLLHGDQLTITGRTIAEELEHLESQGELPDDEEIEADFLYTVDEPKNEEGAIRILTGNLAPGGSVLKVTGDDDLHHEGPVRVFEGEEDAMKYVQEGNVESGDVIAIRNEGPRGGPGMREMLGVTAAVAGQDHAEDVALITDGRFSGATRGFSIGHVAPEAYTGGPIGALEDGDVITVDIADRTLEVDLTDEEIERRLEERDEPEPNYTSGVMAKYGQSFGSAENGAVTNPGAKED",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have 2 iron, 2 sulfur cluster binding, magnesium ion binding to facilitate valine biosynthetic process.2. The protein must exhibit the following characteristics: Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo- 3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3- dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.; Functions in the biosynthesis of branched-chain amino acids.3. The protein with the reaction (2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-   oxopentanoate + H2O, the Proton acceptor must be optimized to ensure efficient catalysis.4. The protein design should emphasize Polar residues, Basic and acidic residues compositional bias.5. We require a protein that can specifically bind to the metabolites produced in the Amino-acid biosynthesis, L-valine from pyruvate: step 3/4 pathway. The designed protein sequence is ",
    "response#1": "RPLVGIVCSYNEIVPGHMNLDKIANAVKMGVAMAGGTPIMFPAIAVCDGIAMGHIGMKYSLVTRDLIADSTEAMAMAHQFDALVMIPNCDKNVPGLLMAAARINVPTVFVSGGPMLAGHVKGQKRSLSSMFEAVGSYAAGTMDECDVCEFENKVCPTCGSCSGMYTANSMNCLTEVLGMGLQGNGTIPAVYSERIRLAKHAGMQVMEMYRRNIRPRDIMTKEAILNALTVDMALGCSTNSMLHLPAIAHEIGMDFEIDFANGISEKTPNLCHLAPAGPTYMEDLNEAGGVYAVMNELNKKGLLYTDCMTVTGKTVGEDVIRPWNNPVYPEGHLAILKGNLAAEGAVAKISGVKKPKMTGPARVFESEEACLDAILAGKISAGDVVIVRYEGPKGGPGMREMLAPTSAIIGAGLGDSVGLITDGRFSGGTYGLVVGHVAPEAFVGGTIALVNEGDSVTIDAEKRLLQLNVSDEELATRRAHWTPPKPRYQRGILGKYAKLVSSSSLGGCDKITPGMLMTNDVIRPWNNPMYAQGHLAILKGNLATEGAVAKITGVKNPEITGLARVFDSEEACLEAILAGKIQAGNIIVVRYEGPKGGPGMREMLAPTSAIIGAGLGDKVGLITDGRFSGGTYGMVVGHVAPEAAVGGTIALVKEGDMITIDAHKRLLQLNISDEELEKRRQVWKPRKPQYTRGVLAKYAKLVSSSSVGAVT",
    "response#2": "KPQVGVASSWNEVTPCNYHLDKLAALAKEGVRQGDCVPIEFTTIAVSDGIAMGHEGMKASLVSRDVIADSVELVMHAERMDALVGIAGCDKSEPGMLMAMARLNLPSVYLYGGTILPGTYGGRDVTIQDVFEAVGAYARGTMSDEDLRGIERAACPTAGSCAGMYTANTMAAAAEALGMSLPGAASPPAVDYRREVLARESGLAVTRCLELGVRPRDVMTREAFENAIAVVMALAGSTNAVLHLLAIAHEAGVPLELDDFDRISRRTPHLVDVRPAGRFVMSDLDRVGGVQVVMRELLEAGLLHGDAPTITGRTVAEADIVTAAQTLLDVVYPANKPITPTGGVVGLRGNLAPEGAIVKVAGMVNQKFTGPARCFDNEELCFEAVSKREYEEGDVLVIRYEGPTGGPGMREMLATTAALYGQGMGDKVALITDGRFSGATRGFCIGHVGPEAALGGPIGLLKDGDIIEIDAVEGVLAVKLSDEELEARRKEWKPRETDYASGAIWRYAQTVGSARYGDIVAPVDRPITPTGTLAILKGNLAPEGAVVKTALVRHLCHEGPARVFDGEQTAFGAARRREIEPGDVVVIRYEGPKGGPGMREMLAMTAALVGQGLGEKVALITDGRFSGATRGLMVGHVAPEAWDGGLIALLRDGDPIEIDVPKRIVRTGLTKKETDARRKKWRRPPPRYRTGALAKYAKLVSSAAVGLKPGESTDIVAPVDRPITPTGTLAILKGNLAPEGAVVKTALVRHLCHEGPARVFDGEQTAFGAARRREIEPGDVVVIRYEGPKGGPGMREMLAMTAALVGQGLGEKVALITDGRFSGATRGLMVGHVAPEAWDGGLIALLRDGDPIEIDVPKRIVRTGLTKKETDARRKKWRRPPPRYRTGALAKYAKLVSSAAVG",
    "response#3": "KPIIAVVNSFTQFVPGHVHLKDLGQMVAEQIQAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVKFRGNEKAIDLVDAMVVAADDSYTDEEVQAFERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSIVATHANRKKLFLKAGELIVSLAKRYYEQDDSSILPRSIATKAAYENAMTLDIAMGGSTNTVLHLLAAASEAEVDFTMDDIDRLSRNVPVLCKVAPAKQDVHMEDVHRAGGIMSILGELDRAKLLNTTVSTVHEKTLQESLAIVKCDKNVPGLLMKPIIAIANSFTQFVPGHVHLKDLGQLVAREIERVGGVAKEFDTIAVDDGIAMGHDGMLYSLPSREIIADSVEYMVNAHCADALVCISNCDKITPGMLMAALRLNIPTVFVSGGPMEAGKTKLADHNLDLIDAMVIAADDSASDEKVAEFERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGTVVATHADREQLFLRAGRVAVELCHRWYGGEDPTALPRGIATFEAFENAMTLDIAMGGSTNTILHLLAAAQEGEVPFGMRDIDRLSKRVPQLCKVAPNTPKYHIEDVHRAGGIMSILGELARGGLLHTNAATVHTRTLAEAIASLDNEVIRPIDNPYSQTGGLAVLKGNIAPDGSVVKRSAVVPEMLVHEGPARVFECEDDAIKAIKGGDIHPGDVVVIRYVGPKGGPGMPEMLNPTSAIAGMGLGSSVALITDGRFSGASRGASIGHVSPEAAVGGPVALIEEGDIIEIDIPNLKLNARISDEELARRKEKWQPREPEVTTGYLARYAAMVTSGNRGAVTG"
  },
  {
    "reference": "MAGSAFQAAAVVVAAGRGIRIGGDTPKQYRRVGGRAVLTRTLAALAAHDGIARIQPVIAPDAAAFYEDCLADLPAQARDKLAAPVAGGATRQLSVRAGLDALERAGAPDLVLVHDAARPFVDTALLDRALAAGRDHGAAVPGIPVTDTVKRVEAAEGGIARVAETPAREALRAVQTPQAFAFRLLADAHARAAAAGLDGFTDDGALAEWAGLPVVVFEGDTANRKITQAADLIEADRRFSESEGSTMTATVTRLGTGFDVHAFTEGDHVWLGGIRIPADRGVLAHSDGDVALHALTDALLGAIADGDIGTHFPPSDEQWRGASSDRFLAHACALVRARGGRIDHLDITVLAEAPRIGAHREAIRARIAEICDVPLSAVSIKATTTEKLGFVGRAEGLAAQAAATIRLPEIDPAGFGA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. We are looking for a protein with broad substrate specificity for 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-   erythritol 2,4-cyclic diphosphate + CMP.2. The designed protein should have 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity, metal ion binding, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity to facilitate isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway, terpenoid biosynthetic process.3. A protein that is capable of catalyzing reactions within the isopentenyl diphosphate biosynthesis via DXP pathway, isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6 pathway with high efficiency and specificity.4. The 4-CDP-2-C-methyl-D-erythritol 2-phosphate, a divalent metal cation binding site should be located in a region of the protein that is accessible to the ligand.5. The presence of a divalent metal cation is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MRVGTGFDVHRLADGEDLWLCGVKIDHHQGLAGHSDADVGLHAVVDAILGACARGDIGDHFPPSDPQWKGAASSAFVQHAVKLAGEAGYAIGNVDLTLICEAPKIGPHRGAMAVRLAELLGIPVSAVSVKATTTEKLGFTGRGEGIAAQAVTTLVTGLDHPDVIAAVMEQAGADVNAVDGDAENTKITTPEDFQEADRKMIASQSVRTGMGFDVHRFEPGDAVTLCGVSIPHTARLAGHSDADVGLHALTDAILGAIAAGDIGDHFPPSDPQWKGAASDRFLRHAADLVAARGGAVTHVDVTLICEKPKIGPHKEAMRMTIAGILGVDIDQVSVKATTTEKLGFTGRGEGIAAQAVASVGVIRR",
    "response#2": "RMGQGYDVHAFGPGDHVWLGGHKVAHTKGLVGHSDADVLSHAITDALYGAIGDGDIGSHFPPSDPKWKGADSSIFLAAAAEAVRARGGMIAHIDATVVCENPKVGPHRDAIRASIAEVVKLPLDRVAVKATTSEKLGFTGREEGIATLAIATGNQVWHTNGAAVMEQAGADVNAVDGDAENTKITTPEDFQEADRKMIASQSVRTGMGFDVHRFEPGDAVTLCGVSIPHTARLAGHSDADVGLHALTDAILGAIAAGDIGDHFPPSDPQWKGAASDRFLRHAADLVAARGGAVTHVDVTLICEKPKIGPHKEAMRMTIAGILGVDIDQVSVKATTTEKLGFTGRGEGIAAQAVASL",
    "response#3": "MRVGTGFDVHRLADGEDLWLCGVKIDHHQGLAGHSDADVGLHAVVDAILGACARGDIGDHFPPSDPQWKGAASSAFVQHAVKLAGEAGYAIGNVDLTLICEAPKIGPHRGAMAVRLAELLGIPVSAVSVKATTTEKLGFTGRGEGIAAQAVTTPGSDGDVALHALTDALLQYGAVAAVMEQAGADVNAVDGDAENTKITTPEDFQEADRKMIASQSVRTGMGFDVHRFEPGDAVTLCGVSIPHTARLAGHSDADVGLHALTDAILGAIAAGDIGDHFPPSDPQWKGAASDRFLRHAADLVAARGGAVTHVDVTLICEKPKIGPHKEAMRMTIAGILGVDIDQVSVKATTTEKLGFTGRGEGIAAQAVASLVLNSERTGFGYDVHAFDPAPADKIRLGGIDIPYEKKLLGHSDADVVLHALTDALLGTIGEGDIGQLFPPSDMQWKNADSEIFLKEAVRRVRAREGRIIHADITVIAEAPKIGPHREAMQVRIAGMLGIEPSRVGLKATTSEGLGFTGRKEGIVAQAVASRVGVGTDVHRWAPEDAPRPLWLGGVHWPGERGVAATSDGDVAAHAACNALLSAAGLGDLGAVFGVDRPEMADASGTAMLAEVLRLLTEAGLAVGNVAVQVVAPRPKVGTRRAEMEAALSAALGGAPVSVSAATTDRLGFIGRGEGLVGIATALL"
  },
  {
    "reference": "MNKDNVKLAIAPIGWTNDDMPELGSENTFQQIVSEMALAGFTGSEVGSKYPRDPAVLKPMLDIRGIQICNAWFSTFFANGQREKTIDEFVNHMNFLHAMGAKVIGCSEQSGSIQGLDKPILGDAKPCFSDEEWQRVAEGYNTLGRLAAEKGMQVCLHHHMGTGIQTTAEIDKFMSLVDERVFLLFDTGHAWYSEGGEAPMLAILKKYLPRINHVHLKDVRPPVIDQVRRDGLSFLDGVKKGTFTVPGDGVIDFRPVFKLLDDFGYKGWMVVEAEQDPALANPFEYAVKARKYIRETAGI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. We are looking for a protein with broad substrate specificity for scyllo-inosose = 3D-3,5/4-trihydroxycyclohexane-1,2-dione +   H2O.2. The designed protein must possess manganese ion binding, myo-inosose-2 dehydratase activity to support inositol catabolic process under certain conditions.3. The protein must be engineered to have a high affinity for Co(2+), Mn(2+) in order to increase its catalytic activity.4. The protein must exhibit the following characteristics: Catalyzes the dehydration of inosose (2-keto-myo-inositol, 2KMI or 2,4,6/3,5-pentahydroxycyclohexanone) to 3D-(3,5/4)- trihydroxycyclohexane-1,2-dione (D-2,3-diketo-4-deoxy-epi-inositol).. The designed protein sequence is ",
    "response#1": "MALAGFKGTEIGNKYPKDVKVLKRALEMRNLQIASAWFSSFLTTKPYEETEKEFIAHRDFLHAMGSKVIVVSEQGHSIQGQMETPIFDGKHHFNEEEWNLLAHGLNKLGQLAADKEMRIVYHHHMGTGVQTTEEIDKLMSVTDENLVYLLFDTGHLVYSGENPVEILKKYVQRIKHVHLKDIRPEIVSKVKNEKLSFLKGVRAGAFTVPGDGSIDFEPIFKILAENNYEGWLMIEAEQDPSIANPLEYAIKGRQYIKAGYKGTEVGNKYPKDPKTLKHFLDLRHLKIASAWFSAFLTTKPYEETEAAFIKHRDFLHAMGAKVIVVAEQGHSVQGLLDKSVFDDKPHFTDDEWERLATGLERLGDRAREVGMQIVYHHHMGTGVQTTAEIDRLMTMTDPDKVSLLFDTGHLVLSGEDPLTIFNRYQDRIKHIHFKDVRQQQADEEHKDHLSFLAGVKNGMFTVPGDGMIDFKPIWEAIQESGYDGWIIVEAEQDPAKANPW",
    "response#2": "MALAGFKGTEIGNKYPKDVKVLKRALEMRNLQIASAWFSSFLTTKPYEETEKEFIAHRDFLHAMGSKVIVVSEQGHSIQGQMETPIFDGKHHFNEEEWNLLAHGLNKLGQLAADKEMRIVYHHHMGTGVQTTEEIDKLMSVTDENLVYLLFDTGHLVYSGENPVEILKKYVQRIKHVHLKDIRPEIVSKVKNEKLSFLKGVRAGAFTVPGDGSIDFEPIFKILAENNYEGWLMIEAEQDPSIANPLEYAIKGRQYIKAGYKGTEVGNKYPKDPKTLKHFLDLRHLKIASAWFSAFLTTKPYEETEAAFIKHRDFLHAMGAKVIVVAEQGHSVQGLLDKSVFDDKPHFTDDEWERLATGLERLGDRAREVGMQIVYHHHMGTGVQTTAEIDRLMTMTDPDKVSLLFDTGHLVLSGEDPLTIFNRYQDRIKHIHFKDVRQQQADEEHKDHLSFLAGVKNGMFTVPGDGMIDFKPIWEAIQESGYDGWIIVEAEQDPAKANPW",
    "response#3": "MALAGFKGTEIGNKYPKDVKVLKRALEMRNLQIASAWFSSFLTTKPYEETEKEFIAHRDFLHAMGSKVIVVSEQGHSIQGQMETPIFDGKHHFNEEEWNLLAHGLNKLGQLAADKEMRIVYHHHMGTGVQTTEEIDKLMSVTDENLVYLLFDTGHLVYSGENPVEILKKYVQRIKHVHLKDIRPEIVSKVKNEKLSFLKGVRAGAFTVPGDGSIDFEPIFKILAENNYEGWLMIEAEQDPSIANPLEYAIKGRQYIKAGYKGTEVGNKYPKDPKTLKHFLDLRHLKIASAWFSAFLTTKPYEETEAAFIKHRDFLHAMGAKVIVVAEQGHSVQGLLDKSVFDDKPHFTDDEWERLATGLERLGDRAREVGMQIVYHHHMGTGVQTTAEIDRLMTMTDPDKVSLLFDTGHLVLSGEDPLTIFNRYQDRIKHIHFKDVRQQQADEEHKDHLSFLAGVKNGMFTVPGDGMIDFKPIWEAIQESGYDGWIIVEAEQDPAKANPYYPL"
  },
  {
    "reference": "MEKMNITNQQHDAFVKSHPNGDLLQLSKWADTKKLTGWYSRRIAVGENGQIKGVGQLLFKKIPKLPYTLCYVSRGFVADYNNKEVLEALLSYAKEVAKDEKSYAIKIDPDVEVDKGAEALKNLRELGFKHKGFKEGLSKDYIQPRMTMITPIDKTDDELVQSFERRNRSKVRLALKRGTKVERSNREGLKIFANLMKITGERDGFLTRDISYFENIYDALHEDGDAELFLVKLEPKPVLDTVNQDLEAQLAEKEKLQSKKQDKKTLNKLNDIDNKIKKTNELKSDLTELEKSEPEGIYLSGALLMFAGNKSYYLYGASSNDYRDFLPNHHMQFEMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKRVWGTYLSEKIGEFDYVLNQPLYHLVEKVKPRLTKAKIKISRKLKGK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be able to regulate cell wall organization, regulation of cell shape. The designed protein sequence is ",
    "response#1": "VRTGMLSGGSSIITTSYEGTVAAYKFNGEKLWENELSGFMNHDIWVQDINGDGLVEIFAANADGNVYCINSDGSLKWTFGLNEVPMNSVTVISDADKKYVVAGGYDKNLYYISTNGELLK",
    "response#2": "VRTGMLSGGSSIITTSYEGTVAAYKFNGEKLWENELSGFMNHDIWVQDINGDGLVEIFAANADGNVYCINSDGSLKWTFGLNEVPMNSVTVISDADKKYVVAGGYDKNLYYISTNGELLG",
    "response#3": "VRTGMLSGGSSIITTSYEGTVAAYKFNGEKLWENELSGFMNHDIWVQDINGDGLVEIFAANADGNVYCINSDGSLKWTFGLNEVPMNSVTVISDADKKYVVAGGYDKNLYYISTNGELLF"
  },
  {
    "reference": "MGMIISMAMTAVTIWTKTKPGRVSCFIRKNSTMVNAPIGIIGGSGLYQMEALKNVEEVTLDTPFGAPSDSFIVGELAGVRVAFLARHGRGHHLLPSEIPFRANIHGMKQLGVKYLISASAVGSLQAEAKPLDMVVPDQFIDRTRQRISTFFGEGIVAHIGFGNPICPQLAQCLSTAIAGLELEGVTLHDRGTYVSMEGPAFSTIAESNLYRSWGGTVIGMTNLPEAKLAREAEIAYATLALVTDYDCWHPDHDHVTVEMVIGNLQKNAVNAQQVILETVKQLAANPFESIAHRALQYAVLTPPDKFPAATYEKLSLLLGKYYPPAP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. We need a protein that can perform phosphate + S-methyl-5'-thioadenosine = adenine + S-methyl-5-   thio-alpha-D-ribose 1-phosphate with high efficiency.2. A protein that can modulate the overall flux of metabolites through the Amino-acid biosynthesis, S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'- thioadenosine (phosphorylase route): step 1/1, L-methionine biosynthesis via salvage pathway pathway, enhancing or suppressing its activity as needed.3. The protein must exhibit the following characteristics: Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.; Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine; Catalyzes the reversible phosphorylation of S-methyl-5'- thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate; Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis.4. The protein should be able to bind phosphate, substrate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MNKIGIIGGSGVYGVFEPKEVVKVHTPYGRPSAPIEIGEIEGVEVAFIPRHGKYHEFPPHQVPYRANIWALHELGVERVIAINAVGSLKEEYKPGDIVIIDQFIDFTKKREYTFYNGPKVAHVSMADPFCPELRKIFIETAKELNLPVHERGTYVCIEGPRFSTRAESRMFRQFADVIGMTLVPEVNLARELGMCYVNISTVTDYDVWAEKPVDAQEVLRVMKENEEKVQKLLKRALKLLGKDTSPRLKIGIIGGSGLDDSQILESRSEKVVSTHFGNPSDVLIEGKIAGVDCVLLARHGRNHSIMPSNVNYRANIWALKTVGCTHVIVSTATGSLQERIHPGDLVIPDNFIDRTTKRAQTFYDGNDMLVGVCHVPMEPAFCSRTREVLIETAKELGLVGVHNKGTVVTIEGPRFSSKAESNLFRQWGADLVNMTLVPEVVLAKEAGLCYAAIAMATDYDCWRDCGENVNVADVMATFKKNVTKVTQLITAVETLKKNNPIIDEEIKKIGIIGGSGLDDSQILESRSEKVVSTHFGNPSDVLIEGKIAGVDCVLLARHGRNHSIMPSNVNYRANIWALKTVGCTHVIVSTATGSLQERIHPGDLVIPDNFIDRTTKRAQTFYDGNDMLVGVCHVPMEPAFCSRTREVLIETAKELGLVGVHNKGTVVTIEGPRFSSKAESNLFRQWGADLVNMTLVPEVVLAKEAGLCYAAIAMATDYDCWRDCGENVNVADVMATFKKNVTKVTQLITAV",
    "response#2": "MKIGIIGGSGLDDSQILESRSEKVVSTHFGNPSDVLIEGKIAGVDCVLLARHGRNHSIMPSNVNYRANIWALKTVGCTHVIVSTATGSLQERIHPGDLVIPDNFIDRTTKRAQTFYDGNDMLVGVCHVPMEPAFCSRTREVLIETAKELGLVGVHNKGTVVTIEGPRFSSKAESNLFRQWGADLVNMTLVPEVVLAKEAGLCYAAIAMATDYDCWRDCGENVNVADVMATFKKNVTKVTQLITAVPHIIAAEALFKEKIGIIGGSGVYGVFEPKEVVKVHTPYGRPSAPIEIGEIEGVEVAFIPRHGKYHEFPPHQVPYRANIWALHELGVERVIAINAVGSLKEEYKPGDIVIIDQFIDFTKKREYTFYNGPKVAHVSMADPFCPELRKIFIETAKELNLPVHERGTYVCIEGPRFSTRAESRMFRQFADVIGMTLVPEVNLARELGMCYVNISTVTDYDVWAEKPVDAQEVLRVMKENEEKVQKLLKRADKEGALLI",
    "response#3": "MKIGIIGGSGVYGVFEPKEVVKVHTPYGRPSAPIEIGEIEGVEVAFIPRHGKYHEFPPHQVPYRANIWALHELGVERVIAINAVGSLKEEYKPGDIVIIDQFIDFTKKREYTFYNGPKVAHVSMADPFCPELRKIFIETAKELNLPVHERGTYVCIEGPRFSTRAESRMFRQFADVIGMTLVPEVNLARELGMCYVNISTVTDYDVWAEKPVDAQEVLRVMKENEEKVQKLLKRAQEVGDGNLNLVFKIFDKQGISRIVVKQALPYVRCVGESWPLTLDRARLEAQTLVAHYQHCPAHTVKIHHYDPTLAVMVMEDLSDHRIWRGELIKGVHYPQAARQLGEYLAQTLFHTSDFYLHPHAKKAEVARFINPEMCEITEDLFFNDPYQVHERNNYPAELEADVAALRDDAALKCAVAALKHRFFSHAEALLHGDIHSGSIFVADGSLKAIDAEFGYFGPVGFDVGTAIGNLLLHPTYIDLLDGSDVGLLKEKSIIDTYRVMRPGEPPTKEASAVLFDQIFSSLTYNAARYNSKLPGVFSSLEGTVIDPSKTLASQEAINLIKGILESR"
  },
  {
    "reference": "MQVSFACTEQTLRSRTSEDRLCPSRPSGGQNGVSMAQTKQRTPPMGAKNHNQVLPLLSHILRESTVKSTGMKYRNLGKSGLRVSCLGLGTWVTFGSQISDEVAENLMTVAYEHGVNLFDTAEVYAAGRAERTLGKILKKKEWRRSSYVVTTKIYWGGQAETERGLSRKHIIEGLRGSLERLQLDYVDIVFANRMDPNSPMEEIVRAMTFVINQGMAMYWGTSRWSAMEIMEAYSVARQFNLIPPVCEQAEYHLFQREKVETQLPELYHKIGVGSMTWSPLACGLITGKYTDTVPEKSRASFKGYHWLKEKAISQEGKKQHSKVKELHPIADRLNCTVTQLAIAWCLRSEGVSSVLLGVSNIEQLLENLGAIQVLPHLTPQLVTEIDQILGNKPNLKKDSRA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The ideal protein would be one that plays a critical role in regulation of ion transmembrane transport through voltage-gated potassium channel activity within membrane, cytoplasm.2. For general function, the protein need meet that Alters functional properties of Kv1.1 and Kv1.2.; Accessory potassium channel protein which modulates the activity of the pore-forming alpha subunit The designed protein sequence is ",
    "response#1": "TWDGFILLATLYVAVTVPYSVCVSTAREPSAARGPPSVCDLAVEVLFILDIVLNFRTTFVSKSGQVVFAPKSICLHYVTTWFLLDVIAALPFDLLHAFKVNVYVGAHLLKTVRLLRLLRLLPRLDRYSQYSAVVLTLLMAVFALLAHWVACVWFYIGQQEIESSESELPEIGWLQELARRLETPYYLVSRSPDGGNSSGQSENCSSSSSSSGSGGGRGSEANGTGLELLGGPSLRSAYITSLYFALSSLTSVGFGNVSANTDTEKIFSICTMLIGALMHAVVFGNVTAIIQRMYARA",
    "response#2": "RVVAFASLFFILVSITTFCLETHEAFNIDRNVTEIHRVGNITSVRFRREVETEPILTYIEGVCVMWFTLEFLVRIVCCPDTLDFVKNLLNIIDFVAILPFYLEVGLSGLSSKAARDVLGFLRVVRFVRILRIFKLTRHFVGLRVLGHTLRASTNEFLLLIIFLALGVLIFATMIYYAERIGARPSDPRGNDHTDFKNIPIGFWWAVVTMTTLGYGDMYPKTWSGMLVGALCALAGVLTIAMPVPVIVNNFGMYYL",
    "response#3": "TWDGFILLATLYVAVTVPYSVCVSTAREPSAARGPPSVCDLAVEVLFILDIVLNFRTTFVSKSGQVVFAPKSICLHYVTTWFLLDVIAALPFDLLHAFKVNVYVGAHLLKTVRLLRLLRLLPRLDRYSQYSAVVLTLLMAVFALLAHWVACVWFYIGQQEIESSESELPEIGWLQELARRLETPYYLVSRSPDGGNSSGQSENCSSSSSSSGSGGGRGSEANGTGLELLGGPSLRSAYITSLYFALSSLTSVGFGNVSANTDTEKIFSICTMLIGALMHAVVFGNVTAIIQRMYARAK"
  },
  {
    "reference": "MYKDLKFPVLIVHRDIKADTVAGERVRGIAHELEQDGFSILSTASSAEGRIVASTHHGLACILVAAEGAGENQRLLQDVVELIRVARVRAPQLPIFALGEQVTIENAPAESMADLHQLRGILYLFEDTVPFLARQVARAARNYLAGLLPPFFRALVEHTAQSNYSWHTPGHGGGVAYRKSPVGQAFHQFFGENTLRSDLSVSVPELGSLLDHTGPLAEAEDRAARNFGADHTFFVINGTSTANKIVWHSMVGREDLVLVDRNCHKSILHSIIMTGAIPLYLTPERNELGIIGPIPLSEFSKQSIAAKIAASPLARGREPKVKLAVVTNSTYDGLCYNAELIKQTLGDSVEVLHFDEAWYAYAAFHEFYDGRYGMGTSRSEEGPLVFATHSTHKMLAAFSQASMIHVQDGGTRKLDVARFNEAFMMHISTSPQYGIIASLDVASAMMEGPAGRSLIQETFDEALSFRRALANVRQNLDRNDWWFGVWQPEQVEGTDQVGTHDWVLEPSADWHGFGDIAEDYVLLDPIKVTLTTPGLSAGGKLSEQGIPAAIVSRFLWERGLVVEKTGLYSFLVLFSMGITKGKWSTLVTELLEFKRCYDANLPLLDVLPSVAQAGGKRYNGVGLRDLSDAMHASYRDNATAKAMKRMYTVLPEVAMRPSEAYDKLVRGEVEAVPIARLEGRIAAVMLVPYPPGIPLIMPGERFTEATRSILDYLEFARTFERAFPGFDSDVHGLQHQDGPSGRCYTVECIKE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that can perform lysine decarboxylase activity in cytoplasm to support amino acid metabolic process.2. For general function, the protein need meet that Plays an essential role in lysine utilization by acting as a lysine decarboxylase. The designed protein sequence is ",
    "response#1": "LDLDWGLVKEARESAKKIAADAQVFIDAHSTVTVERTICRLLGIDGVDEFGVPLPNVIVDFIKDNGNISLGVAKYIGNAMIETKLQPQEIAEKVAKKELDITKMQWHDDFDIQLALKDITHSTVERIKANRKAREDYLEQFGGDKKGPYIYVIVATGNIYEDVTQAVAAARQGADVVAVIRTTGQSLLDFVPFGATTEGFGGTMATQENFRIMRKALDDVGVELGRYIRLCNYCSGLCMPEIAAMGALERLDMMLNDALYGILFRDINMKRTLVDQFFSRIINGFAGVIINTGEDNYLTTADAIEEAHTVLASQFINEQFALVAGLPEEQMGLGHAFEMEPGTENGFLLELAQAQMAREIFPKAPLKYMPPTKFMTGNIFKGHIQDALFNIVTITTGQKVHLLGMLTEAIHTPFMSDRALSIENARYIFNNLKDFGNDIEFKKGGIMNTRAQEVLKKAAELLKTIETMGIFKTIEKGVFGGVRRPIDGGKGLAGVFEKDNTYFNPFIR",
    "response#2": "SVALAKAGLPQPKTALATDREEALRLMEAFGYPVVLKPVIGSWGRLLAKVTDRAAAEALLEHKEVLGGFQHQLFYIQEYVEKPGRDIRVFVVGERAIAAIYRRSAHWITNTARGGQAENCPLTEEVARLSVKAAEAVGGGVVAVDLFESERGLLVNEVNHTMEFKNSVHTTGVDIPGEILKYAL",
    "response#3": "PFFRALVEHTAQSNYSWHTPGHGGGVAYRKSPVGQAFHQFFGENTLRSDLSVSVPELGSLLDHTGPLAEAEDRAARNFGADHTFFVINGTSTANKIVWHSMVGREDLVLVDRNCHKSILHSIIMTGAIPLYLTPERNELGIIGPIPLSEFSKQSIAAKIAASPLARGREPKVKLAVVTNSTYDGLCYNAELIKQTLGDSVEVLHFDEAWYAYAAFHEFYDGRYGMGTSRSEEGPLVFATHSTHKMLAAFSQASMIHVQDGGTRKLDVARFNEAFMMHISTSPQYGIIASLDVASAMMEGPAGRSLIQETFDEALSFRRAR"
  },
  {
    "reference": "MNGSRQYILAAGGTGGHMIPAHALAVELMRRGHHVALVTDERGTRFPELFKDVQIHQLPAGRLTGGVKGLFQAMRNIWAGRERALTLYENFTPAAVVGFGGYPALPALLAAFKAKIPTVIHEQNAVMGRTNRFLAGRVDAIATAYHQVDRLKKRYRRKTEVTGNPVRDEVLFLRDLPYPPLSDNSIFRILVVGGSQGASILSEVVPEGLGLLPLHLRRRLQVTQQCRPEDLEKTRAQYAKLGIPADISTYMADLPQRLGWSHLVISRAGASTIAELGVAGRPAILIPYPAAMDNHQYANARELVSAGGARLIDQRRFNPFELAKQIQKMALEPSALKNAAARARQVGYPDAVEKLADLVERVGGNLPQQNSIEEDSTFEKNQEGAVA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be optimized for UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase activity activity in order to improve cell wall organization, cell division.2. For general function, the protein need meet that Cell wall formation3. The designed protein should interact with enzymes and substrates within the Cell wall biogenesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MSGIAGLLLSLGHTVSGSDKADTKETERLIKLGLTFCGAHSPEQVNGVDLVVYSSAIKQGNVVYDKAKELGIPMIRRAEALAAIMSETRSIGITGTNGKTTTSYVLESILKEEGFEKLLVFGGSQGAHALNMRVIEALPHLADLRERIQITHQTGARDREYVEKGYRACGFTPDVREFIDDMSAAYAGCDLVVCRAGATTLAELTVCKKPSILVPFPAAADNHQVKNARSLVDAGAAVMIEERDLTGEVLAREIREILDAPERRERMARAAGRLGSPQAAKEILSLLVG",
    "response#2": "MSGIAGLLLSLGHTVSGSDKADTKETERLIKLGLTFCGAHSPEQVNGVDLVVYSSAIKQGNVVYDKAKELGIPMIRRAEALAAIMSETRIAKQSTPTMGGIVLGAITGTNGKTTTSYVLESILKEEGRNVGVIGTINYRYKGKSFQASLTTPESIDLMKIMGEMRDAGMTDIIIEVSSHSLEQGRVRGLNWSRALFTNFSRDHLDYHSTMQKYFKAKSLLFSSLREEGKVKAIINMDDPKGRVLKKITKSSTVSYGLGDNCLIRAADINFSAEGLHFRLTTPTGDIPITSTLLGQVNVYNILAAAAVAVGEGVPLETAVVSLEKFPGVPGRIEHIGATKAGAHVFVDYAHTPDGLDVLLRAARPHAPGRIVLVFGCGGDRDTGKRPKMGAIAAKFADVVIVTDDNPRSEEPADIRKQILAATPGAMEIGDRAQAIREGVAMLKAGDALIIAGKGHEDYQDIGGVKHHFDDREVARRALALRP",
    "response#3": "MSGIAGLLLSLGHTVSGSDKADTKETERLIKLGLTFCGAHSPEQVNGVDLVVYSSAIKQGNVVYDKAKELGIPMIRRAEALAAIMSEHLGKRNAVNLTDGLDGLTVLVVGGSGGALKINRAVVEILNQYQPNKYRLLLVTGKRLYKSTLESINAESLQSKHKVFAYVNDMPHALKACDLIVCSAGAITIAEVTAVGKASILIPKAHTAENHQEYNANAMGNKGAAVVIREDELSGEILNKKIQDIIGNIQVVKKMEAASYKEGIRDAADRLAKLL"
  },
  {
    "reference": "MPTRLTKTRKHRGNVSAGKGRIGKHRKHPGGRGKAGGQHHHRTNLDKYHPGYFGKVGMRYFHKQQNHFWRPEINLDKLWTLVDSEKKDEYLSKSSASAAPVIDTLAHGYGKVLGKGRLPEVPVIVKARFVSKLAEEKIRAVGGVVELVA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should exhibit Basic residues compositional bias.2. A protein with RNA binding, structural constituent of ribosome functions is required to enable translation.3. The protein must exhibit the following characteristics: The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules; Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell; The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.. The designed protein sequence is ",
    "response#1": "LEDIYTSAHEAIRADPAFKPTEKKFTKEQYAAESKKYRQTKLSKEERAARVAAKIAALS",
    "response#2": "LEDIYTSAHEAIRADPAFKPTEKKFTKEQYAAESKKYRQTKLSKEERAARVAAKIAALH",
    "response#3": "PIGCKVTLRGEAAEGFLETSLGIVEKRLNESQFDIFGNVSFGVEEHTDYPGMRYDPNIGIFGMDITVVVNRPGYRVSKRRIAKRKIPTSHKITKEDTIL"
  },
  {
    "reference": "MLKTDVVVIGAGPAGSMAAKHAALGGANVVLIDKKSEIGTPKRCAEGVYDEGFKWLNIEPDERWIAQRIYGATLHSPDGNSFTVTSEDVPVQGFILERKVFDKHMAMDAARAGAKIMIKTLVTDIKRNENGFILSCDSIEGTIEVEAKIAICADGPESIMAKKLGINSTTPQNMMSCAQFEMCNVDYNDDEKIEFYLGQDVALKGYAWIFPKGNGVANVGLGVTGNTDKTAYEYIMDFIEKCPATKNAQPIEMNIGGDPINGLIEKIYDDNLLICGDAAGQVNPIEGGGIIEGMLGGMAAGDVAAKAIKEENYSKERLHEYYEKYSELSFNLIETLPAARDIVYSFTDDEYNKIISVANTIDLKNISKRDVLKVVFKLPPTLTTKLIKLLKIVFPKQMKSILF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Is involved in the reduction of 2,3- digeranylgeranylglycerophospholipids (unsaturated archaeols) into 2,3- diphytanylglycerophospholipids (saturated archaeols) in the biosynthesis of archaeal membrane lipids2. The designed protein must possess FAD binding, geranylgeranyl reductase activity, oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor to support glycerophospholipid metabolic process under certain conditions.3. The protein have enhanced 2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 A = 2,3-bis-   O-(geranylgeranyl)-sn-glycerol 1-phosphate + 8 AH2. The designed protein sequence is ",
    "response#1": "MCTCAQYEMVGVDVEEDATHFFVDAEFFPGGYFWIFPKGEGRANVGLGIRGSESEPGDALKVLNRALEDHELISEAVADAVPVEVNVGGICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGKICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGRICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGRICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGRICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGREYDVIVIGAGPGGSMASYHSSINGAKTLLIDKSQEIGTPVRCAEAVPYLDEFGINPDPSFIRSKIDGGILVAPNGKKIIVKGGKTQGYVVERKIFDKHLAVRSANVGTKIAIKSRVVGLDYDGEKYTVIVNHLGQIYAIKAKIVIAADGVESSIAEMAGIKCKKL",
    "response#2": "MCTCAQYEMVGVDVEEDATHFFVDAEFFPGGYFWIFPKGEGRANVGLGIRGSESEPGDALKVLNRALEDHELISEAVADAVPVEVNVGGICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGKICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGKICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGKICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGKICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGKICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGKICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGSAICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGSAICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGS",
    "response#3": "MCTCAQYEMVGVDVEEDATHFFVDAEFFPGGYFWIFPKGEGRANVGLGIRGSESEPGDALKVLNRALEDHELISEAVADAVPVEVNVGGICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGNICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGNICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGNICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGNICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGNICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGDICSCAEYEMTNVKLLDNEMMEFYFGDICPKGYIWLFPKGDTVNVGIGIIDSKKRALDYLDEFLTNPLVEGEA"
  },
  {
    "reference": "MALEVLEALDSARTQWYHVTAIVIAGMGFFTDAYDLFCITTVSKLLGRLYYFDPSTGKPGKLPNNVNNLVTGVALVGTLSGQLFFGYLGDKLGRKKVYGVTLILMVACAICSGLSFGASAKSVMGTLCFFRFWLGFGIGGDYPLSATIMSEYANKRTRGAFIAAVFAMQGVGIIFAGLVSMCLSAGFKASYHAPSFHDDPIMSTQPQGDLMWRLVLMIGAVPAAMTYYWRMKMPETGRYTAIIEGNAKQAAADMARVLDIEIQAEQDKLAEFKAANDYPLWSNEFFTRHGRHLIGTMTSWFLLDIAFYSQNLTQKDIFPAMGLIDKDFEMNAIQEVFETSRAMFVIALFGTFPGYWFTVFFIEKLGRYKIQLIGFFMMSVFMFIIGVKYDYLRNENSHMFALLYGLTFFFANFGPNSTTFVLPAELFPTRVRSTCHALSAAAGKAGAMVGAFGIQNYTQKGEQKQIKHAMMILAVTNLIGFFCSFLVTETKGRSLEEISGEDGRESELTPTPPNNRVPTRQEPRSETM",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should contain a Helical; Name=12, Helical; Name=2, Helical; Name=3, Helical; Name=7, Helical; Name=6, Helical; Name=1, Helical; Name=9, Helical; Name=10 membrane-spanning region.2. The desired protein should be able to perform NH4(+)(in) = NH4(+)(out)3. For general function, the protein need meet that Low-affinity transporter for external inorganic phosphate (Pi) probably involved in the acquisition of phosphate released by arbuscular mycorrhizal (AM) fungi (e.g; Acts as a Pi- sensing machinery at the root tip level, independently of AM fungi, involved in the regulation of early root branching and lateral roots formation.4. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.5. A protein that contributes to detection of phosphate ion, lateral root formation, cellular response to phosphate starvation, arbuscular mycorrhizal association by functioning in plasma membrane, periarbuscular membrane with inorganic phosphate transmembrane transporter activity. The designed protein sequence is ",
    "response#1": "MKFGKDFVRQMIPEWQQAYMDYAGLKSILQEIQTSRKRSERPGILKRKLSGSRNFSGLTKRYSRTASTREPEIQDILVHATTGDDGFERYETTILEVAEAGRESELAFFKTLDLEFDKVNHFYRSKVEEMVKEAVVLNKQMDALIAFRIKVERPSSSWSCSETVSVDMNALDSNDQRNTLAEEMGIRVEGNGSNGGDSTKESVPQVLSVLERIRLNKTQETPLSTIKNVLKLSNQEELKFTRENLKKIEERLKNVFIEFYRKLRHLKNYSFLNTLAISKIMKKYDKIASRSAAKPYMEMLEGSVTATSQRHPLFRGMDDLILDNICDRAKPRVFSKDEKIIREGDPVQRMIFIMRGRVKRIQSLSKGVLATSTLEPGGYLGDELLSWCLRRPFLDRLPPSSATFVCLENIEAFSLGSEDLRYLEAQAELRLLRQKGEIMAIMGPSGAGKSTFLDALAGRIAKGSLQGSVRIDGKPVTTSYMKMVSSYVMQDDQLFPMLTVFETFMFAAEVRLPPSISRDEKKKRVHELLNKLGLQSATHTYIGDEGRRGVSGGERRRVSIGIEIIHKPSLLFLDEPTSGLDSTSAYSVVEKIKDIAQGGSIVLMTIHQPSFRIQMLLDKITILARGRLIYMGRPQS",
    "response#2": "MKFAEHLSAHITPEWRKQYIQYEAFKEMLYAAQDQAPSIEVTDEDTVKRYYAKFEEIFFQTCEKELAKINTFYSEKLAEAQRRSATLQNELQSSLDAQRESSAIPGLRQRRKAVFALTHEERVQHRNIKDLKLAFSEFYLSLILLQNYQNLNFTGFRKILKKHDKILETSRGADWRPKGSKRSSQSGTWILMRRNFTNIRRTPELFLSRLMVLTFMGVMMATMFHNPKNTLQGITNRLSFFIFTVCLFFFSSNDAVPAFIQERFIFIRETSHNAYRASCYTIASLITHMPFLALQALAYAAIVWFALELRGPFIYFFLVLFISLLSTNSFVVFVSSIVPNYILGYAAVIAFTALFFLFCGYFLSSEDIPLYWRWMNKVSTMTYPYEGLLMNEMFPLYSLFAFVVLHMIMYASNIYFWKRYRVNYPFIFGFKEGTELGYRHVLLLSFGLGTLALCAVLINLDMEMDPNTNDYKTMTELLPMFILALVVAILFCPFNIFYRSSRVFFLMVVFRCIAAPLYKVNLPDFFLADQLTSQVQALRSLEFYICYYGWGDFKHRQNTCRSSDVYSTFYFIVAVIPYWSRFLQCVRRLIEENDSSQGYNALKYLLTVVAVCLRTAYSFNRGNIWKISAWVFSALATFYGTYWDIVFDWGLLHRPSKHLLREKLLVPHKAVYYVAIVLNIVLRMAWLQTVLDFNLSFLHRETMIALLAALEIIRRGIWNFFRLENEHLV",
    "response#3": "MFPLYSLFAFVVLHMIMYASNIYFWKRYRVNYPFIFGFKEGTELGYRHVLLLSFGLGTLALCAVLINLDMEMDPNTNDYKTMTELLPMFILALVVAILFCPFNIFYRSSRVFFLMVVFRCIAAPLYKVNLPDFFLADQLTSQVQALRSLEFYICYYGWGDFKHRQNTCRSSDVYSTFYFIVAVIPYWSRFLQCVRRLIEENDSSQGYNALKYLLTVVAVCLRTAYSFNRGNIWKISAWVFSALATFYGTYWDIVFDWGLLHRPSKHLLREKLLVPHKAVYYVAIVLNIVLRMAWLQTVLDFNLSFLHRETMIALLAALEIIRRGIWNFFRLENEHKFGKDFVRQMIPEWQQAYMDYAGLKSILQEIQTSRKRSERPGILKRKLSGSRNFSGLTKRYSRTASTREPEIQDILVHATTGDDGFERYETTILEVAEAGRESELAFFKTLDLEFDKVNHFYRSKVEEMVKEAVVLNKQMDALIAFRIKVERPSSSWSCSETVSVDMNALDSNDQRNTLAEEMGIRVEGNGSNGGDSTKESVPQVLSVLERIRLNKTQETPLSTIKNVLKLSNQEELKFTRENLKKIEERLKNVFIEFYRKLRHLKNYSFLNTLAISKIMKKYDKIASRSAAAKIGLEDAFKYLNCSEHGLSGAEAEARLKQHGPNKLPDNSRNPVLVYFGYMWNPLAWAMEAAAIIAYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAFGGSSEGFIGRHNFALRDFPTPTADYSFFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFVYPVVSHWFWSPDGWASPFRSADDRLFSTGAIDFAGSGVVHMVGGIAGLWGALIEGPRRGRFEKGGRAIALRGHSASLVVLGTFLLWFGWYGFNPGSFTKILVPYNSGSNYGQWSGIGRTAVNTTLSGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITAGCSVVEPWAAIVCGFMASVVLIGCNKLAELVQYDDPLEAAQLHGGCGAWGLIFVGLFAKEKYLNEVYGATPGRPYGLFMGGGGKLLGAQLVQILVIVGWVSATMGTLFFILKRLNLLRISEQHEMQGMDMTRHGGFA"
  },
  {
    "reference": "MIKGCGCSDGLTDRTRYEEVVLDTMLYSAQLKERVARQNSAVIVAMARMIAGTFEDGGKVLLCGNGGSAADAQHLATELTIRYRSSVERPALPAIALSTDTSALTAGANDLGYDAVFARLVEAYGREGDILLGLSTSGNSQSVINALDFARQQGMKTLALLGGNGGLMKGLADLELIVPHSGSADRVQECHITIGHVLIDLVERMLGYCRL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a fast turnover rate for 2 D-sedoheptulose 7-phosphate = D-glycero-alpha-D-manno-   heptose 7-phosphate + D-glycero-beta-D-manno-heptose 7-phosphate.2. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects.3. The designed protein must be optimized for D-sedoheptulose 7-phosphate isomerase activity.4. The protein must bind to Zn(2+) in order to perform its enzymatic function.5. We require a protein that can specifically bind to the metabolites produced in the D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7- phosphate: step 1/1 pathway.6. The designed protein have SIS domains with specific sequence motifs that are crucial for its activity.7. For general function, the protein need meet that Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate. The designed protein sequence is ",
    "response#1": "MEEASAVLSNFIEQEKKQNSIAKAADVITSSLKQGGKIISCGNGGSSCDAMHFAEELTGRYRENRKAIPAISISDPSHMSCVANDYGYKFVFSRYLEALGNKGDVLLAISTSGNSENVLEAVKEAKNKGMFVVALTGKDGGEMAGMLPNGVEIRVPSTSTARIQEVHLLVIHAISCLVDEQLAGS",
    "response#2": "MVNALINGNKILSCGNGGSAAHAQSFASQMLNRYERERPSLPAIALSTDTSTMTSIANDYSYDEVFSKQVRALGQTGDILLAISPNGASRSVISAMEAALSRDMTIVALTGLDGGEMAGLLGPNDVEIRVPSSRAVRIHEVHLLVIHNLCEGVDDCLFSGDSEKLLALIETLENQGKILICGNGGSASDAQHFATELTGRYKLERKGLSAISLNTDTSALTAIANDYGYEEVFARQVEALGVKNDVLIGISTSGNSKNVLKAYEKAKDLGMKTLSLAGRDGGKMKPLSDMALIVPSDDTPRIQEMHILMIHILCDCIERHFAHKPETVVVMTPELCVALYRGDKKTILAGNGGSAADAQHIAAELVGRYGFDRPSIPSLALTTDTSCLTAIGNDYGYDNVFSRQLEGMGQAGDIFIGISTSGNSKNIINAFISAKKKGITTVALVGRDGGEMAKMADVALVVPSDSTPRIQESHILIGHIICDIIEKEIFGDGVGV",
    "response#3": "MILIAGYRAGARVFMCGNGGSAADAQHFAAELTGHLIFDRPPLGAEALHANSSHLTAVANDYDYDTVFARALEGSARPGDTLFAISTSGNSMSVLRAAKTARELGVTVVAMTGESGGQLAEFADFLINVPSRDTGRIQESHIVFIHAISEHVEHALFAPRLCVALYRGDKKTILAGNGGSAADAQHIAAELVGRYGFDRPSIPSLALTTDTSCLTAIGNDYGYDNVFSRQLEGMGQAGDIFIGISTSGNSKNIINAFISAKKKGITTVALVGRDGGEMAKMADVALVVPSDSTPRIQESHILIGHIICDIIEKEIFGDGVLLCVALYRGDKKTILAGNGGSAADAQHIAAELVGRYGFDRPSIPSLALTTDTSCLTAIGNDYGYDNVFSRQLEGMGQAGDIFIGISTSGNSKNIINAFISAKKKGITTVALVGRDGGEMAKMADVALVVPSDSTPRIQESHILIGHIICDIIEKEIFGDGVLQAA"
  },
  {
    "reference": "MTETFQHYTVMLKETVDGLNIKPDGIYVDCTLGGAGHSEYLLSQLNTNGHLYAFDQDQKAIDHAKVRLAPYIEKGMVTFIKANFRELEAKIKEHVPQVDGILYDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYDVVNEYSYNELVKIFFRYGEEKFSKQIAREIERVRKIHPIQTTGELVEIIKDVIPAPARRKGGHPAKRIFQAIRIAVNDELGAEEASLEQAIRLLKINGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVVPEEFQPELKLINRKPILPSEEELSENNRSRSAKLRIAEKIRVKEE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. We are looking for a protein with broad substrate specificity for cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = H(+) +   N(4)-methylcytidine(1402) in 16S rRNA + S-adenosyl-L-homocysteine.2. The designed protein must be able to perform rRNA (cytosine-N4-)-methyltransferase activity under specific conditions.3. The protein should have a high affinity for S-adenosyl-L-methionine.4. For general function, the protein need meet that Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA. The designed protein sequence is ",
    "response#1": "MLYIVGTPIGNLEDITYRAVDVLKSVNVIFAEDTRVTIKLLSRYKINKKMISCNAVTENKKIRLLLDYLAKGDSVAFVSDAGTPGLSDPGSLLVAAAFREGYKVCPIPGVSSFNTIVSVNPFRDKSVLFEGFLPNKGLKRFKKIAELYKRGDAFVLLESGHRLLKLLVEISSVSLDAKILIGREMTKILNCKKDVDLEKVKGIINKLI",
    "response#2": "MLYIVGTPIGNLEDITYRAVDVLKSVNVIFAEDTRVTIKLLSRYKINKKMISCNAVTENKKIRLLLDYLAKGDSVAFVSDAGTPGLSDPGSLLVAAAFREGYKVCPIPGVSSFNTIVSVNPFRDKSVLFEGFLPNKGLKRFKKIAELYKRGDAFVLLESGHRLLKLLVEISSVSLDAKILIGREMTKIVRNRW",
    "response#3": "MLYIVGTPIGNLEDITYRAVDVLKSVNVIFAEDTRVTIKLLSRYKINKKMISCNAVTENKKIRLLLDYLAKGDSVAFVSDAGTPGLSDPGSLLVAAAFREGYKVCPIPGVSSFNTIVSVNPFRDKSVLFEGFLPNKGLKRFKKIAELYKRGDAFVLLESGHRLLKLLVEISSVSLDAKILIGREMTKILMARDSDAGTPGLSDPLKMLYIVGTPIGNLEDITYRAVDVLKSVNVIFAEDTRVTIKLLSRYKINKKMISCNAVTENKKIRLLLDYLAKGDSVAFVSDAGTPGLSDPGSLLVAAAFREGYKVCPIPGVSSFNTIVSVNPFRDKSVLFEGFLPNKGLKRFKKIAELYKRGDAFVLLESGHRLLKLLVEISSVSLDAKILIGREMTKILNSRL"
  },
  {
    "reference": "MTISVEKPIFEEVSAFEKSGDNIGELKLDGGFSMPKMDTNDDEAFLAPEMNAFGRQFRDYDVESERQKGVEEFYRLQHINQTVDFVKKMRAEYGKLDKMVMSIWECCELLNEVVDESDPDLDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIHDLGKVITLPQFGGLPQWAVVGDTFPVGCAFDESNVHHKYFVENPDFHNETYNTKNGIYSEGCGLNNVMMSWGHDDYMYLVAKENGSTLPSAGQFIIRYHSFYPLHTAGEYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVDVEKVKPYYMSLIKKYFPENLRW",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Involved in the biosynthesis of UDP-glucuronic acid (UDP- GlcA), providing nucleotide sugars for cell-wall polymers.2. The protein should be able to bind Fe cation ligand in a variety of conditions3. The desired protein should be able to perform myo-inositol + O2 = D-glucuronate + H(+) + H2O4. We require a protein that can specifically bind to the metabolites produced in the D-glucuronate from myo-inositol: step 1/1 pathway.5. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm that is critical for inositol catabolic process. The designed protein sequence is ",
    "response#1": "ALAGFTGSEVGSKYPRDPAVLKPMLDIRGLEIVNAWFSTFFANGDREKTLDEFINHRDFLHAMGAKVIGCSEQSLSIQGTAKAVLEEKPHFSDEQWRLTVEGYNQLAKLAAEKGMTVGLHHHMGTAIQTCAEVDRFMAQTHDDVYLLFDTGHAYYSEGSQQAMLAMLEKHLARINHVHLKDVRDEVVAEVKAQRLSFLEGVKRGTFTVPGDGVIDFDPVFKILDDSGYRGWMVVEAEQDPARANPFEYALKARRYIR",
    "response#2": "ALAGFTGSEVGSKYPRDIDVLKRKLDLRGIQICNAWFSTFFVDGKKEETIKGFIEHRDFLHAMGAKVIGCSEQSRSIQGQKKAIFKEKTIFTEAEWQLLAEGYNELAKLAAEKGMKVCLHHHMGTGIQTPAEIDKYMEITNDDVYLLFDSGHLYYSEGSQQVMLEVLEKYIHRVVHVHLKDVRDEVVAEVKANDLSFLEGVVKGTFTVPGDGVIDFKPIFDILEKYDYKGWMVVEAEQDPAIANPLEYAIKGRQYIRRRELGIAPDELVLTFVGRIQPHKAPDLLLRAAAPLIHAHPDRRIRILVVGGPSGTGLERPDALIALARELGIEHAVTFEPPRPPAGLAEVYRASDLVVVPSYSESFGLVAVEAQACGTPVVAAKVGGLSVAVADGVSGRLIDGHDPQEWTAVLDELTANAELRTELAAGAA",
    "response#3": "SGFKGTEVGNKYPRDTKVLKRALELRDMQIASAWFSAFLTTKPYEETEKAFIKHRDFLYEMRSKVIVISEQGHSIQGKMETPIFEEKPIFTEEEWKDLAKGLNKLGKLAKEKNMKVVYHHHMGTGVQTTEEIDKLMEMTDPTLVYLLYDTGHLVFSGEDPIVVLRKYINRIKHVHLKDIREDVVKIVKQKKMSFLQAVKLGAFTVPGDGDIDFKPVFNILAENNYEGWLLVEAEQDPARANPLEYAIKARKGQTKIKSVLAQFLVDAGIKPLSIASYNHLGNNDGYNLSSPKQFRSKEISKQSVVDDIIESNELLYNKESGDKVDHCIVIKYLPAVGDSKVAMDEYYSELMLGGHNKISIHNVCEDL"
  },
  {
    "reference": "MHAAAHALTRAAVEEIRAVRGAVPGARVLLLVGGGHNGGDALLAGALLARRGCAVTAAPAAEISRLHAAALADACAAGVRLAADPIAAARAAAGGAAGADLVVDGLTGIGATGPLRPAAAALIAPLCAAGDPGERPFRVLAVDLPSGVGVDDGTLPGPALAADRTVTFTCFKAAHLAPPAAPRCGRVDVVDLGLPVPNGAPLAVRPTDTELGGLLRVPGDRDHKYTRGVVGLWAGSQTYPGAGVLAASAAVRTGAGMVRLAAPARVVDLVLARRPEVVPADGRCQALVLGPGTDPADAPRAAELDAALGRVLGEAGEPVDAVIDAGALPLLAARVVSGGRCMPRQVLTPHAGEAAALLGALGEQTSRESVEAAPAAAARRLAELTGATVLLKTTPTLIASPGGTLLSVDSGPGWLATAGSGDVLAGIIGALLAARRADAENGKAVGGEDVQAEAPARCAALGVRLHAVAAARAAGLVDASPAVTAGAGRLFACSGHPLAALDLTEAIPAAWNHLWSIGPHRALNSPRAGTAPGHACRGKNVHP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. For general function, the protein need meet that Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP; Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.3. The designed protein should have ATP binding, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADHX epimerase activity, metal ion binding to facilitate nicotinamide nucleotide metabolic process.4. The protein have enhanced (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.5. The (6S)-NADPHX binding site should be located in a region of the protein that is accessible to the ligand.6. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MQEMDRKTIQELGIPGIVLMENAGRGAAHTFLSHFSPEQGARVIIICGRGNNGGDGYVVGRYLHEAGLRPTIMVLSEKEKISGDARMNLDIIHGMGLDIRYLPNEQSWQDQKKELTHCNYLVDAILGTGLNSPVRGYYGAVIDEINSSGKPVMAIDIPSGLNADNGAIMGLAVRADLTVTFGLPKLGQLVFPGCDMVGRLVRIDICIPHSVATPVFCRQHLPPRDPSSHKGTYGHVLALGGSPGFTGAITLTARAALRAGAGLVTAAVPRGCQPVVAAGAPEIMTWGLPETAGGSLGRSALDPLLERLENFSVLAIGPGLGRDPETVELVKSLLPRASIPLVVDADALNALATDTGILSQPHGPMVLTPHPGEMGRLIGCPAAKIQEDRLNIARRYAQEWRCVLLLKGARTVVACPDGRAYINPTGNPGMATGGSGDVLTGIIAGLIAQGMSLDKAAAIGAYLHGAAGDLAAEELGQEALIAGDILHFLPRAFKSF",
    "response#2": "MRQIENQAFGAGISSDHMIQTVGLKIANILDSDCSDKNKMVLGLIGKGNNGSDTLAILTYLAQKGWETFAWLLSKRESEDRYLKDFLNVSGKAVDYENDTEESQLDNWLKQSSIILDGIYGIGFHLPLGEAFRPIFRKINQRKNPSLIYAIDCPSGMDCDTGKIDPDCIRANRTICIEAVKIGEVLPPANLLVGELETISLDLPQDLTLALGGSPGFTGAITLTARAALRAGAGLVTAAVPRGCQPVVAAGAPEIMTWGLPETAGGSLGRSALDPLLERLENFSVLAIGPGLGRDPETVELVKSLLPRASIPLVVDADALNALATDTGILSQPHGPMVLTPHPGEMGRLIGCPAAKIQEDRLNIARRYAQEWRCVLLLKGARTVVACPDGRAYINPTGNPGMATGGSGDVLTGIIAGLIAQGMSLDKAAAIGAYLHGAAGDLAAEELGQEALIAGDILPSSHKGTYGHVLALGGSPGFTGAITLTARAALRAGAGLVTAAVPRGCQPVVAAGAPEIMTWGLPETAGGSLGRSALDPLLERLENFSVLAIGPGLGRDPETVELVKSLLPRASIPLVVDADALNALATDTGILSQPHGPMVLTPHPGEMGRLIGCPAAKIQEDRLNIARRYAQEWRCVLLLKGARTVVACPDGRAYINPTGNPGMATGGSGDVLTGIIAGLIAQGMSLDKAAAIGAYLHGAAGDLAAEELGQEALIAGDWL",
    "response#3": "MRQIENQAFGAGISSDHMIQTVGLKIANILDSDCSDKNKMVLGLIGKGNNGSDTLAILTYLAQKGWETFAWLLSKRESEDRYLKDFLNVSGKAVDYENDTEESQLDNWLKQSSIILDGIYGIGFHLPLGEAFRPIFRKINQRKNPSLIYAIDCPSGMDCDTGKIDPDCIRANRTICIEAVKIGEVLPPANLLVGELETISLDLPQDLTNANKGTFGKVLTVAGSVNYSGAAYLACSGAMRAGAGLVTLAVPVNLQPVLAGKLNEVTYLPLSESVKGVVSGEASFIVLSSLHEYTTLLIGCGLGQHDMTVSFTKGILFGNKPIMPLVIDADALNVLAKTNDWWQKLMMDAILTPHPGEMSRLTGIKIEEIQSNRIDTAMLFAQKWGKTVVLKGAYTVIASADGRCRVSPFANPGMASAGTGDVLAGIIAGLTAQGLDNFNAACLGVWLHGQAGEIVKAELGDTGMLPSSHKGTYGHVLALGGSPGFTGAITLTARAALRAGAGLVTAAVPRGCQPVVAAGAPEIMTWGLPETAGGSLGRSALDPLLERLENFSVLAIGPGLGRDPETVELVKSLLPRASIPLVVDADALNALATDTGILSQPHGPMVLTPHPGEMGRLIGCPAAKIQEDRLNIARRYAQEWRCVLLLKGARTVVACPDGRAYINPTGNPGMATGGSGDVLTGIIAGLIAQGMSLDKAAAIGAYLHGAAGDLAAEELGQEALIAGDCL"
  },
  {
    "reference": "MRIRVSYLLVLCFTLIWFRWTVVYSSFSDLDALLKLKESMKGAKAKHHALEDWKFSTSLSAHCSFSGVTCDQNLRVVALNVTLVPLFGHLPPEIGLLEKLENLTISMNNLTDQLPSDLASLTSLKVLNISHNLFSGQFPGNITVGMTELEALDAYDNSFSGPLPEEIVKLEKLKYLHLAGNYFSGTIPESYSEFQSLEFLGLNANSLTGRVPESLAKLKTLKELHLGYSNAYEGGIPPAFGSMENLRLLEMANCNLTGEIPPSLGNLTKLHSLFVQMNNLTGTIPPELSSMMSLMSLDLSINDLTGEIPESFSKLKNLTLMNFFQNKFRGSLPSFIGDLPNLETLQVWENNFSFVLPHNLGGNGRFLYFDVTKNHLTGLIPPDLCKSGRLKTFIITDNFFRGPIPKGIGECRSLTKIRVANNFLDGPVPPGVFQLPSVTITELSNNRLNGELPSVISGESLGTLTLSNNLFTGKIPAAMKNLRALQSLSLDANEFIGEIPGGVFEIPMLTKVNISGNNLTGPIPTTITHRASLTAVDLSRNNLAGEVPKGMKNLMDLSILNLSRNEISGPVPDEIRFMTSLTTLDLSSNNFTGTVPTGGQFLVFNYDKTFAGNPNLCFPHRASCPSVLYDSLRKTRAKTARVRAIVIGIALATAVLLVAVTVHVVRKRRLHRAQAWKLTAFQRLEIKAEDVVECLKEENIIGKGGAGIVYRGSMPNGTDVAIKRLVGQGSGRNDYGFRAEIETLGKIRHRNIMRLLGYVSNKDTNLLL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.2. For general function, the protein need meet that Involved in the autoregulation of nodulation (AON), a long distance systemic signaling from root to shoot and back again, which allows legumes to limit the number of root nodules formed based on available nitrogen and previous rhizobial colonization; Involved in the regulation of root colonization by arbuscular mycorrhizal (AM) fungi.; LRR receptor kinase involved in the regulation of root and shoot growth, and root nodule organogenesis3. The protein should contain a Helical membrane-spanning region.4. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.5. The protein contains novel Protein kinase domains that confer a unique function or activity.6. Ensure the presence of a signal peptide within the protein.7. The ideal protein would be one that plays a critical role in cell differentiation through ATP binding, protein serine kinase activity within plasma membrane. The designed protein sequence is ",
    "response#1": "DGEIMAVLGASGSGKSTLIDALANRIAKESLHGSVTINGESIDSNLLKVISAYVRQEDLLYPMLTVEETLMFAAEFRLPRSLPTREKKKRVKELIDQLGLKRAANTIIGDEGHRGVSGGERRRVSIGVDIIHNPIMLFLDEPTSGLDSTSAFMVVTVLKAIAQSGSVVVMSIHQPSYRILGLLDRLLFLSRGEVVHRDIKPGNILDLFRGMDDLILDNICDRAKPRVFSKDEKIIREGDPVQRMIFIMRGRVKRIQSLSKGVLATSTLEPGGYLGDELLSWCLRRPFLDRLPPSSATFVCLENIEAFSLGSEDLRYITDHFRYKFANERLKRTARGVIHRDIKPSNLLDLLDGISGEARDGEIMAVLGASGSGKSTLIDALANRIAKESLHGSVTINGESIDSNLLKVISAYVRQEDLLYPMLTVEETLMFAAEFRLPRSLPTREKKKRVKELIDQLGLKRAANTIIGDEGHRGVSGGERRRVSIGVDIIHNPIMLFLDEPTL",
    "response#2": "LETVKVIGKGSGGVVQLVRHKWVGKFFAMKVIQMNIQEEIRKQIVQELKINQASSQCPHVVVCYHSFYHNGAFSLVLEYMDRGSLADVIRQVKTILEPYLAVVCKQVLLGLVYLHNERHVIHRDIKPSNLLVNHKGEVKISDFGVSASLASSMGQRDTFVGTYNYMSPERISGSTYDYSSDIWSLGMSVLECAIGRFPYLESEDQQNPPSFYELLAAIVENPPPTAPSDQFSPEFCSFVSACIQKDPPARASSLDLLSHPFIDTWEQEED",
    "response#3": "LEVQVRKSDGISKGAYQVAKWNGTRVSVKILDKDSYSDPERINAFRHELTLLEKVRHPNVIQFVGAVTQNIPMMIVVEYNPKGDLSVYLQKKGRLSPSKALRFALDIARGMNYLHECKPDPIIHCDLKPKNILLDRGGQLKISGFGMIRLSKISQDKAKVANHKAHIDLSNYYIAPEVYKDEIFDLRVDAHSFGVILYEITEGVPVFHPRPPEEVARMMCLEGKRPVFKTKSRSYPPDIKELIEKCWHPEAGIRPTFSEIIIRLDKILFRGMDDLILDNICDRAKPRVFSKDEKIIREGDPVQRMIFIMRGRVKRIQSLSKGVLATSTLEPGGYLGDELLSWCLRRPFLDRLPPSSATFVCLENIEAFSLGSEDLRYLETVKVIGKGSGGVVQLVRHKWVGKFFAMKVIQMNIQEEIRKQIVQELKINQASSQCPHVVVCYHSFYHNGAFSLVLEYMDRGSLADVIRQVKTILEPYLAVVCKQVLLGLVYLHNERHVIHRDIKPSNLLVNHKGEVKISDFGVSASLASSMGQRDTFVGTYNYMSPERISGSTYDYSSDIWSLGMSVLECAIGRFPYLESEDQQNPPSFYELLAAIVENPPPTAPSDQFSPEFCSFVSACIQKDPPARASSLDLLSHPFL"
  },
  {
    "reference": "MESALLLPSLLLVAAYPRGGSPQQEWMQSPPAPSVTSAPFWVRLNPELEAVPPGGSAWLNCSHNCPLPVHSSLRTQLRQGKIVNGSGWVSYQLLDVRAWNSKVRCVVTCAGETREATARITAYKRPRSVILEPPVLVGHKYTLRCYVTHVFPVGFLVVSLRRGGRVIYHESLERFTGSDLANVTLTYVMRAGLNDLWQPLTCHARLNLDGLVVRSSSAPVMLTVLALSPASIALASTSIATLVGILLAVGAVYVRKYLAVQT",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that can perform integrin binding in plasma membrane to support cell adhesion.2. The protein must exhibit the following characteristics: Adhesion molecule that binds to leukocyte adhesion LFA-1 protein LFA-1 (integrin alpha-L/beta-2); ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins ; Isoform 2 may modulate binding of membrane-associated ICAM4..3. The designed protein must have at least one Ig-like C2-type domain.4. The designed protein should contain Extracellular, Cytoplasmic topological domains. The designed protein sequence is ",
    "response#1": "ARPVITVNAGLEVYPRYLFNNSYCDYCSDGKGALPRKLNFQVELLLDKLKQKGAIRRALFLHSRSPSHSKNMTISRGGLMQCEELIAYLRDESEFRDKLTPITIFMEYRLDYRTAADTTGLQPILNQFTPANISRQAHILLL",
    "response#2": "RPIVSASASLTIFPAMFNPEERSCSLEGNPVACINLSFCLNASGKHVPDSIGFTVELQLDWQKQKGGVRRALFLASRQAAC",
    "response#3": "PVLKVGVAMRFSPVEVAKAVYRCWEEKPSALEAGDATVCLTIQKSSLDQLGDIQSSVRFDLALDPGRLTSRAIFNETKNPTLTRRKTLGLGIHCETLKLLLPDCVEDVVSPIILHLNFSLVREPIPSPQNLRPVLAVGSQDLFTASLPFEA"
  },
  {
    "reference": "MTILSEIVDYKEQLLKDGYYHDKLQNLKGVKHKNKSRLTDALIKNDNLTLIAEIKSKSPSVKAFQQTNIIKQVSDYERYGANAISVLTDERYFGGSFERLQQISETTQLPVLCKDFIIDPLQIDVAQKAGASIILLIVNILTDEKLRQLYQYASSKGLEVLVEVHDGIELQRAYQLNPQIIGVNNRDLKSFNTDVKHTNQILKCKKENYLYISESGIHSQQEVKQIVKSGIDGLLVGGALMNCNELEHFIPSLKLKKVKQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should interact with enzymes and substrates within the Amino-acid biosynthesis pathway to enhance or regulate its activity.2. A protein with indole-3-glycerol-phosphate synthase activity functions is required to enable tryptophan biosynthetic process.3. We are looking for a protein with broad substrate specificity for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+)   = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O. The designed protein sequence is ",
    "response#1": "LDEILKKTREDLKRRKKMLSFELLGRSLSANPYMPRDVIKALKSTPNEPFKLICEIKKASPSKGVIREYFKPVEIAREYEKAGADAFSVLTEPHFFKGDLEFISQIRRYTKMPILRKDFIIDEYQILEALVYGADFVLLIAKALSLKELKKLLEFTHHIGLEALVEVHDKKDLLNATLSGANIIGINHRDLNDFSLHMNLAEELVPLIPNGKIIVAESGLNSREQLVNLNKVGVDAFLIGEHFMRQNDICAAVREL",
    "response#2": "LDEIVDYKRNLIKEGYYEEKLKTLNEVDITHKSSFKSQLDESNQLAVIAEIKSKSPTLDQLPNRDLAQQVKDYEANGANAVSILTDEHYFGGSYERLQDLTLQTTLPVLCKDFVVDKIQIDVAKKAGASIILLIVNVLTDQQMKDLYQYATSLNLEVLVEVHDKEELERAYKLKPQIIGVNNRDLKRFVTDVLHTNEILENKKEGYYYISESGIRDEQDVANVVESGIDGLLIGESLMKCEDLSQFR",
    "response#3": "MVIFGLSRAIRKAKKNPIIAEIKVYSPKYGDLLRGRDPLRILRAYEEAGAVGISYITDQKYFKGSFDFLKVLCKETTLPVLRKDFITSKEEIEKTAEVGASAVLLITRILKDKLPEFVDYAKEHGLDTLVEVHTEDELKLAIKTNSTMIGINNRDIGKLEMDDGDVSLTEILAPKIPGKFVKVSESGIATLEDLKRALRVADAALIGTALMKAENPAELLE"
  },
  {
    "reference": "MENRWQVMIVWQVDRMRIRTWNSLVKHHMYISKKAKGWVYRHHYESRHPRVSSEVHIPLEDDSKLVIVTYWGLHTGERDWHLGHGVSIEWRQKRYSTQVDPNLADQLIHQHYFDCFSESAIRKALLGQVVRPRCEYQAGHNKVGSLQYLALKALVPPTKKRPPLPSVRKLTEDKWNEPQKTKGHRESPTMNGH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should include a HCCH motif motif to ensure proper function.2. For general function, the protein need meet that Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G; In turn, they are directed to the 26S proteasome for degradation; Recruits an active E3 ubiquitin ligase complex composed of elongin BC, CUL5, and RBX2 to induce polyubiquitination of APOBEC3G and APOBEC3F; May interact directly with APOBEC3G mRNA in order to inhibit its translation; Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions3. The protein can be expressed in plasma membrane and perform RNA binding without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "MEPVDPRLEPWNHPGSQPKTACNNCYCKKCCFHCQVCFIRKGLGISYGRKKRRQRRGTPQGSKDHQNPVPEQPLPTTRGNPTGPKESKKKVERETETDPVH",
    "response#2": "MENRWQVMIVWQVDRMRIRTWNSLVKHHMYISKKAKRWLYRHHYESRHPKVSSEVHIPLGDARLVITTYWGLHTGERDWHLGHGVSIEWKQRRYSTQVDPDLADQLIHLHYFDCFSDSAIRKAILGQVVRPRCDYQAGHNKVGSLQYLALTALIKPKKIKPPLPSVKKLTEDRWNKPQKTKGHRGSHTMNGH",
    "response#3": "MENRWQVMIVWQVDRMRIRTWNSLVKHHMYVSKKAKGWFYRHHYESTHPKISSEVHIPLGDARLVITTYWGLHTGERDWHLGQGVSIEWRKRNYKTQVDPDLADQLIHLHYFDCFSDSAIRIRHGCEYPSGHNKVGSLQYLALKALVTPKRIKPPLPSVRKLTEDRWNKPQKTGHRGSHTMNGH"
  },
  {
    "reference": "MQFTALLAALGAPLALAASIPAAAHNHSMIDVQLAATGNSMIKATITNTGDRTLNLLKFNTIMDEHPTRKVMVYQDGAEVQFTGMLPRYKMSDLTPEYFVNLGPKASVEHSFDLAATHDLSRGGKIVVKAHGMVPTAEENATTITGHTLYESNELTMDVDGKQAAAVEQAMGGDDSTGVIDKRSNIVTSSCRGSQLRVLQTALSNSARLSRAAASAAQRNPSKMREYFKTADSRTVQKVASRFLSVARESSSGSTGRTTYYCNDNRGGCHPGVLAYTLPSKNQVFNCPSYYQLPALNNRCHGQDQATTTLHELTHNPAVVTPFCEDLGYGYQRVSALPASKAIQNADTYSLFANGM",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that contributes to proteolysis by functioning in extracellular region with metalloendopeptidase activity, metal ion binding.2. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding.3. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.4. The protein must exhibit the following characteristics: May be involved in virulence.; Probable secreted metalloprotease that shows high activities on basic nuclear substrates such as histone and protamine .5. The protein must contain a signal peptide for proper functionality.6. We need a protein that can perform Preferential cleavage of bonds with hydrophobic residues in   P1'. Also 3-Asn-|-Gln-4 and 8-Gly-|-Ser-9 bonds in insulin B chain. with high efficiency. The designed protein sequence is ",
    "response#1": "AGLLYQGEPGALNESISDIMGAMADRDDWEIGEDVYTPGIAGDSLRSLEDPSKQGNPDHYSNRYTGTEDYGGVHINSSIHNKAAYLLAEGGVHHGVQVEGIGREASEQIYYRALTYYVTASTDFSMMKQAAIEAANDLYGEGSKQSASVEKAYEAVGG",
    "response#2": "PRTCDPRLTFDAITTLRGEILFFKDKYFWRKHPQLPTAELNFISLFWPSLPNGIQAAYEDFERDLVFLFKGNQYWALNGYEIQQGYPRDISNYGFPSTVQAVDAAVFYRRKTYFFVNDQFWRYDNQRQFMEPGYPKSISTAFPGIESRVDAVFHRDYVFVFFSGPRYYAFDLNTRRVIRIDRSNKWLNC",
    "response#3": "PRTCDPRLTFDAITTLRGEILFFKDKYFWRKHPQLPTAELNFISLFWPSLPNGIQAAYEDFERDLVFLFKGNQYWALNGYEIQQGYPRDISNYGFPSTVQAVDAAVFYRRKTYFFVNDQFWRYDNQRQFMEPGYPKSISTAFPGIESRVDAVFHRDYVFVFFSGPRYYAFDLNTRRVIRIDRSNKWLNC"
  },
  {
    "reference": "MAGAEQSQRWSLKAKTVLVTGGTKGIGHAIVEEFAGFGAVIHTCARNEYELNECLSKWQKKGFQVTGSVCDASLRPEREKLMQTVSSMFGGKLDILINNLGAIRSKPTLDYTAEDFSFHISTNLESAYHLSQLAHPLLKASGCGNIIFMSSIAGVVSASVGSIYSATKGALNQLARNLACEWASDGIRANAVAPAVIATPLAEAVYDDEFKKVVISRKPLGRFGEPEEVSSLVAFLCMPAASYITGQTICVDGGLTVNGFSYQPQG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit oxidoreductase activity.2. The intended protein structure should align with helix-strand-turn-helix-strand-helix-strand-helix-strand-helix-strand-helix-helix-strand-helix criteria.3. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.4. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate, NADP(+) binding. The designed protein sequence is ",
    "response#1": "PLYGELKTQLATAASLRMDKHRSNSVGPHDLYFTLLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAGSQRETVQAVTDGALIDITDMREWREEN",
    "response#2": "PLYGELKTQLATAASLRMDKHRSNSVGPHDLYFTLLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAGSQRETVQAVTDGALIDITDMREWREEK",
    "response#3": "PLYGELKTQLATAASLRMDKHRSNSVGPHDLYFTLLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAGSQRETVQAVTDGALIDITDMREWREEA"
  },
  {
    "reference": "MVATQQGPDRPGIDDPGRGRRIAIDVGSVRIGVASSDPDGILATPVETVPRSKERGPDAPDIRRIIAIVSEYEAVEVIVGLPQTLRGEQGKAAGIATAFAKRLQRAVEPIPVRLSDERLTTVTAARNLRESGVKARGQRPMIDQAAAVEILQGWLDERSRAVKPGESSKDAALEGDL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECE",
    "response#2": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECQ",
    "response#3": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECR"
  },
  {
    "reference": "MNLKLSTQNFFGTSETELKEIFCKWNVETYRVNQVFTWVYHYNCRDFSEMSNISKSLRSKLAENFHFSLPKIQKRTHSKDGSIKYLMELNDGAIVESVWMPSDSRKTLCLSTQVGCRLNCSFCLTASLGLKRNLTAGEIIGQHIAINEELEEKDQITNVVFMGMGEPLDNYVPVVDALRIMTSPEAMKVSTRKVTVSTSGLVNKIKDFQEEGLHINLAISLNASDNKTRDILMPINKKYPIEELIDCLKKYPLKPQRRHTIEYVLLEGINDSDEDAQRVVKWLQGIPCKINLIPFNFFDSTVYRPPSENRVLEFQSYLMKRNFSAFIRKNRATDILGACGQLAKQA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A key design requirement for the protein is the optimization of the Proton acceptor, S-methylcysteine intermediate to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.2. The protein should have a high affinity for [4Fe-4S] cluster.3. The designed protein should have tRNA binding within cytoplasm to regulate rRNA base methylation. The designed protein sequence is ",
    "response#1": "SQAGCGMACPFCATGQAGLTRNLSTAEIVEQLRLNMALAESGALGESVKVTNVVFMGMGEPMANYPAVRAALHRMIDPSPEGFGMAARHVTVSTVGLVPAILKLAEEGLQVTLAVSLHAPDDGLRDDLIPINSRYKVGELLDAARHYFVRTGRRVSIEYALIKDMNDHQWVTCSEVFADLEVQSVLERGGEPAYRLRQAYAALTGSLVRDWEEATSLPQGLRGALNQEAPAAVLDLRRTWRATDGTRKYLFHTHDGHAIETVMIPEKGRHTVCISTQVGCAMACKFCATGLLGIKRNLGAREIAEQVFVCARDISPERVSNVVVMGMGEPMLNYKATLGALKVLNDPKGFGLAARHIAVSTSGLVDKIRRFADEPEQFHLAISLHTPFEEERREIMPVAARHTIPELMDAARYFVSRTRRKLFFEYTLLAGVNDQPRHVEALAELFDHPLYHLNLLRFNWTDTGFSATSKADAKRFLNHARDLGLSATLRPSRGQDIDAACGQLAARDYQTPIDLFIRQHEYGLSVCVTTQVGCDIGCTFCASGLKRKQRDLTAGEIVAQVMQVQHYLDEKGKGERVSHVVVMGIGEPFDNYDNVMNFLHIINNAKGLAIGARHITVSTSGLAPKIREFADNGLQVNLALSLHAPNDEIRSRMMRINRKHPLEEVMAAIDEYLKKTNRRITFEYIMISGVNDSPEQARELVALLKDKRHLSYVNLIPYNPVAEHIKYERSTQKAMQAFYDILQKNKINCVIRQESHQG",
    "response#2": "QKYGLAVCVTTQVGCNIGCSFCASGLIKKSRDLTSGEIVEQIIKAQQHLDQAEQDERVSHVVVMGIGEPFDNFKNLVDFLQVIKDQKGLAIGAKRITVSTSGLPDKIIEFTDADLQVNLAISLHAPNNELRTQIMVINRAFPIEKLMKAVDYYLAKTNRRIMFEYILLKDVNDRKEHALELAELIRDKQNLCSVNLIPYNPVDEHSQYQRSDQESILAFYDTLKKQDINCTVRLEHGAERNKG",
    "response#3": "SSQVGCTLSCTFCHTGTQKLVRNLGPAEIVGQFMAARDSYGEWPSPSADMPRYLSTIVLMGMGEPLYNYENVAKAMRIIMDGEGIALSRRRITLSTSGVVPMMDRCGDELGINLAISLHAVTNELRDQIVPLNRKYPIEELIAACRRYPAASNSRRITFEYIMLRGVNDSEADEVQSVLERGGEPAYRLRQAYAALTGSLVRDWEEATSLPQGLRGALNQEAPAAVLDLRRTWRATDGTRKYLFHTHDGHAIETVMIPEKGRHTVCISTQVGCAMACKFCATGLLGIKRNLGAREIAEQVFVCARDISPERVSNVVVMGMGEPMLNYKATLGALKVLNDPKGFGLAARHIAVSTSGLVDKIRRFADEPEQFHLAISLHTPFEEERREIMPVAARHTIPELMDAARYFVSRTRRKLFFEYTLLAGVNDQPRHVEALAELFDHPLYHLNLLRFNWTDTGFSATSKADAKRFLNHARDLGLSATLRPSRGQDIDAACGQLAARDEGCEVTQEVQSVLERGGEPAYRLRQAYAALTGSLVRDWEEATSLPQGLRGALNQEAPAAVLDLRRTWRATDGTRKYLFHTHDGHAIETVMIPEKGRHTVCISTQVGCAMACKFCATGLLGIKRNLGAREIAEQVFVCARDISPERVSNVVVMGMGEPMLNYKATLGALKVLNDPKGFGLAARHIAVSTSGLVDKIRRFADEPEQFHLAISLHTPFEEERREIMPVAARHTIPELMDAARYFVSRTRRKLFFEYTLLAGVNDQPRHVEALAELFDHPLYHLNLLRFNWTDTGFSATSKADAKRFLNHARDLGLSATLRPSRGQDIDAACGQLAARD"
  },
  {
    "reference": "MKALDEPPYLTVGTDVSAKYRGAFCEAKIKTAKRLVKVKVTFRHDSSTVEVQDDHIKGPLKVGAIVEVKNLDGAYQEAVINKLTDASWYTVVFDDGDEKTLRRSSLCLKGERHFAESETLDQLPLTNPEHFGTPVIGKKTNRGRRSNHIPEEESSSSSSDEDEDDRKQIDELLGKVVCVDYISLDKKKALWFPALVVCPDCSDEIAVKKDNILVRSFKDGKFTSVPRKDVHEITSDTAPKPDAVLKQAFEQALEFHKSRTIPANWKTELKEDSSSSEAEEEEEEEDDEKEKEDNSSEEEEEIEPFPEERENFLQQLYKFMEDRGTPINKRPVLGYRNLNLFKLFRLVHKLGGFDNIESGAVWKQVYQDLGIPVLNSAAGYNVKCAYKKYLYGFEEYCRSANIEFQMALPEKVVNKQCKECENVKEIKVKEENETEIKEIKMEEERNIIPREEKPIEDEIERKENIKPSLGSKKNLLESIPTHSDQEKEVNIKKPEDNENLDDKDDDTTRVDESLNIKVEAEEEKAKSGDETNKEEDEDDEEAEEEEEEEEEEEDEDDDDNNEEEEFECYPPGMKVQVRYGRGKNQKMYEASIKDSDVEGGEVLYLVHYCGWNVRYDEWIKADKIVRPADKNVPKIKHRKKIKNKLDKEKDKDEKYSPKNCKLRRLSKPPFQTNPSPEMVSKLDLTDAKNSDTAHIKSIEITSILNGLQASESSAEDSEQEDERGAQDMDNNGKEESKIDHLTNNRNDLISKEEQNSSSLLEENKVHAD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. Incorporate a signal peptide in the protein design.2. The protein must exhibit the following characteristics: Plays a role in the regulation of epigenetic modifications at the PWS/AS imprinting center near the SNRPN promoter, where it might function as part of a complex with RB1 and ARID4A; May function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes; Plays a role in myeloid homeostasis where it regulates the histone methylation state of bone marrow cells and expression of various genes involved in hematopoiesis; Involved in spermatogenesis, together with ARID4A, where it functions as a transcriptional coactivator for AR (androgen receptor) and enhances expression of genes required for sperm maturation; Acts as a transcriptional repressor; May function as a leukemia suppressor ..3. Prioritize Polar residues, Basic and acidic residues, Acidic residues compositional bias within the protein sequence to achieve desired characteristics.4. strand-strand-turn-strand-helix-strand-strand-strand-strand-helix-strand-strand-turn is the desired arrangement for the engineered protein.5. The designed protein must have at least one ARID, Tudor-knot domain. The designed protein sequence is ",
    "response#1": "LKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIQDGTIHLYIYSIHSIPKGTEITIKEIMVGSMFQAEIPVGICKYRETEKVYENDDQLLWDPEYVMEERVIDFLNEASRRTCEERGLDAIPEGSHIKDNEQALYELVKCNFDTEEALRRLRFNKEIMVGSMFQAEIPVGICKYRETEKVYENDDQLLWDPEYVMEERVIDFLNEASRRTCEERGLDAIPEGSHIKDNEQALYELVKCNFDTEEALRRLRFNLKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIQDGTIHLYIYSIHSIPKGTEITIPFNEKLLLGVYLKKDCIKGDFIQEILGELDFYKNYLTDPRNHYRIWGTAKRRVIFHSHWPIYIDARLSGNSTRYLRRSCQPNVELVTIKLQDTDNRNDKSSGRKSSRIKFVLRALRDISEDEELYILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIQDGTIHLYIYSIHSIPKGTEITIPFNEKLLNFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIQDGTIHLYIYSIHSIPKGTEITIAFDFDYGN",
    "response#2": "EIEEEKIEDKFLRDDLENKDAGDDDDDGDPAAKREHELLFGRKSTPKNKEKKIKKPEDSERDSDEEEEKSQEREETESRCDSEGEDEEDDTEPCLTGTKVKVKYGRGKTQKIYEASIKSTEMDDGEILYLVHYYGWNVRYDEWVKADRIIWPLDKGGPKKKQKKKVKNKLTVKNLSPVVSNELLEQAFSQFGPVERAVVVVDDRGRATGKGFVEFAAKPPARKALERCKLKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIQDGTIHLYIYSIHSIPKGTEITIPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIQDGTIHLYIYSIHSIPKGTEITIAFSCPLVWTEEECRNFEQGLKAYGKDFHLIQANKVRTRSVGECVAFYYMWNGEYKELQLSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYEAPAAAVRRFGEDTAPPSFPSCGHYREEPALGPAKAARQAARDGARLALARAAPECCAPPTPEAPGAPAQLPFVLLNYHRVLARRGPLGGAAPAASGLACAPGGPEAATGALRLRHPSPAATSPPGAPLPHYLGASVIITNG",
    "response#3": "TNNLLFKPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIQDGTIHLYIYSIHSIPKGTEITIAFDFDYPPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIQDGTIHLYIYSIHSIPKGTEITIAFPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIQDGTIHLYIYSIHSIPKGTEITIAFSSGLSITLGVYLKKDCIKGDFIQEILGELDFYKNYLTDPRNHYRIWGTAKRRVIFHSHWPIYIDARLSGNSTRYLRRSCQPNVELVTIKLQDTDNRNDKSSGRKSSRIKFVLRALRDISEDEELYIETVEEKPQTPVSGATTTTEIEEEKIEDKFLRDDLENKDAGDDDDDGDPAAKREHELLFGRKSTPKNKEKKIKKPEDSERDSDEEEEKSQEREETESRCDSEGEDEEDDTEPCLTGTKVKVKYGRGKTQKIYEASIKSTEMDDGEILYLVHYYGWNVRYDEWVKADRIIWPLDKGGPKKKQKKKVKNKLKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIQDGTIHLYIYSIHSIPKGTEITI"
  },
  {
    "reference": "MFFGTFTPKLDDKGRLTLPAKFREELKDGLMVVKGQDHSLAIYPREEFLLRARKAAAASRSNPQARAFVRNLAASADEQDLDSQGRISVSAAHREYAGLKKECVVIGSVDFLEIWDAQAWEEYSAAHEADFAAGDDEAFANFL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must have at least one SpoVT-AbrB domain.2. A protein that localizes to nucleoid, cytoplasm and can perform DNA-binding transcription factor activity, DNA binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "MFQGASALTLDAKGRMSIPTRHREALQLQAEGRVTVTKHPDGCLMLFPRPEWERFRERIAALPMEAI",
    "response#2": "MFQGASALTLDAKGRMSIPTRHREALQLQAEGRVTVTKHPDGCLMLFPRPEWERFRERIAALPMEAX",
    "response#3": "MSFIGKEKHAVDEKGRLMIPARFRRKFPETSGSLASKKEPASLYVMKSPDSSLELYLPDVWEEMARTISALSDFHPDERLLG"
  },
  {
    "reference": "MARIGESVDLLKCSFCGKSQKQVRKLIAGPRVYICDECIELCNEIIEEELTEVTEADQTELPRPKQVYDHLQEYVIGQEPAKRSLAVAVYNHYKRIRAGVSGHTLSLAGAEGDEEAIEVAKSNILLVGPTGSGKTYLAQSLAKKLDVPFAVADATSLTEAGYVGEDVENILLKLIQAADFDLKKAEQGIIYIDEIDKISRKSENPSITRDVSGEGVQQALLKILEGTVASVPPQGGRKHPQQEFLHIDTTNVLFIVAGAFAGLEEIVQQRTGKKGIGFGAPIRDTADVDIAGQVQPEDLLKFGLIPEFIGRLPVVATLSKLSVADMVRILTEPRNALVKQYRKLFQLDGVELTFDPQALEAIAELALERGTGARGLRAILEDILMPVMFDLPGREDVAAVLVTEDAVAKLADPEIFTHEMIEAERRRHKSA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that It directs the protease to specific substrates; Can perform chaperone functions in the absence of ClpP. The designed protein sequence is ",
    "response#1": "TKTHKTTVSGAWAPLIAEESDKLLDQEALTQEALELAENHGIVFLDEIDKVASSSQRSGADVSREGVQRDLLPLIEGTTVSTKYGPVKTDHILFIASGAFHVAKPSDLLPELQGRLPII",
    "response#2": "EIMAPPGMEEMTNQLQGLFQNLAGDTKKKRKMKIKDALKALVEEEAAKLVNQEELKEQAIYNVENNGIVFIDEIDKICKRGEVSGPDVSREGVQRDLLPLIEGSTVSTKHGMVRTDHILFIASGAFQVAKPSDLIPELQGRLPIL",
    "response#3": "KSNIAMIGPTGSGKTLLAQTLARIINVPFAMADATSLTEAGYVGEDVENILLKLIQAADYDVEKAEKGIIYIDEIDKVARKSENPSITRDVSGEGVQQALLKILEGTVASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIDQVIKRRLGKKVIGFGSHEKQEDLDTGQLLSKVLPEDLLRYGLIPEFIGRIPVH"
  },
  {
    "reference": "MIDIDRQLEHIKRGCAELIDEGELRKKLERGTPLRIKAGFDPTAPDLHLGHTVLIHKLRHFQELGHTVIFLIGDFTGLIGDPSGRSDTRPPLTREQVLANAETYKQQVFKILDPEKTVVDFNSRWMGEFGAADFIRLASRYTVARMMERDDFEKRYKEGRPIAVHEFLYPLVQGYDSVALKADVELGGTDQKFNLLVGRHLQSQYGQEPQCILTMPLLEGLDGVKKMSKSLGNYVGIDESPADMFGKLMSVSDELMWRYFELISSRSLDEIADLRRKVETGEAHPKLVKESLAYELTTRYHGEDKAAEAQQGFNAVFAGGGVPDDAPVHACDHGDDSTPPAFLEAAGLVKSRGEAKRLIKEGALSVDGVRCDDANSPLASGEYVIKLGKKRFLRLTVR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design must feature the 'HIGH' region, 'KMSKS' region motif.2. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.3. The protein must have high specificity towards RNA binding. The designed protein sequence is ",
    "response#1": "MSIQLNEFQKKALQSFLMSDKNLLITAPTGTGKSFLAMLMAMETKSRVVYTVPLRALALQLNDDFHNKVAPLVNGYADSVALTSEVYEEDPENLEERVIFTTYEKADAIFRRHYPWTDRIETLIIDEIHNIGDKERGKAIENLIAYAMNEGIRIVAMSATIPDVNKIAEIIDAEIIKTDERPIPLYKAVKIL",
    "response#2": "MSIQLNEFQKKALQSFLMSDKNLLITAPTGTGKSFLAMLMAMETKSRVVYTVPLRALALQLNDDFHNKVAPLVNGYADSVALTSEVYEEDPENLEERVIFTTYEKADAIFRRHYPWTDRIETLIIDEIHNIGDKERGKAIENLIAYAMNEGIRIVAMSATIPDVNKIAEIIDAEIIKTDERPIPLYKAVKIG",
    "response#3": "MSIQLNEFQKKALQSFLMSDKNLLITAPTGTGKSFLAMLMAMETKSRVVYTVPLRALALQLNDDFHNKVAPLVNGYADSVALTSEVYEEDPENLEERVIFTTYEKADAIFRRHYPWTDRIETLIIDEIHNIGDKERGKAIENLIAYAMNEGIRIVAMSATIPDVNKIAEIIDAEIIKTDERPIPLYKAVKIF"
  },
  {
    "reference": "MIRNVAIMGATGAVGQEILSILEERNFPLQSLKLLASERSAGKEFKFKGETLKCEVLNKDSFKGVDLVLSSAGAAISQEFAPIAVENGAVVVDNTSFFRMDPNVPLVVPEVNPEDIKLHKGIIANPNCTTIMMVVVLNPIEKISHIKKIHISSYQSASGAGAVAMEELKQQYKDILETGSTTHINKFPFQLAYNVIPQIDKMTENDYTKEEMKMFNETRKIMHSDVRTSATCVRVSSLRSHSESVWFETERPVSVEEIRNALKNAPGVTLKDDPQNYVYPMPLESAGKDNIFVGRIRKDLADENSNTLWLTGDQIRKGAALNAVQIGEILLKM",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The substrate, NADP(+), phosphate binding site should be located in a region of the protein that is accessible to the ligand.2. The Proton acceptor, Acyl-thioester intermediate active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. The designed protein should be optimized for NADP binding, aspartate-semialdehyde dehydrogenase activity, protein dimerization activity, NAD binding activity in order to improve lysine biosynthetic process via diaminopimelate, isoleucine biosynthetic process, 'de novo' L-methionine biosynthetic process. The designed protein sequence is ",
    "response#1": "LKPIHEAYGLDRVIVSTYQAVSGAGVEAIEELDQQNASLLKGEKPQAAILPSSGDKNHYQIAYNVIPQIDLFAEEGYTFEEWKMINETKKIMGLPDLKVSATCVRVPVKYGHSESVYIEIDRDDVTVTDLQALLAQADGVIVQDDPANQLYPLATESEDKKETFVGRIRKDPDVANGFHLWIVSDNLIAR",
    "response#2": "KLAVVGATGLVGQEILRVLEERNFPFTELILVASAKSVGHTVKFKGSDYKIISIEDAVKQAPDIAIFSAGGGTSLEWAPKFAEAGTIVIDNSSAWRMDPDKKLVVPEINGHVIGIDDKIIANPNCSTIQMVMALKPLHDEAKLVRMVASSYQAVSGSGLAGVEELANQVRTAGDRGPELTHDGSAVDLGEPAKYARPIAFNVLPMAGSIVDDGSHETDEEQKLRNESRKILDIPELLVSGTCVRVPVFTGHALSINVEFDRPLSVERATELLSTAAGVRLSDIPTPLQAAGEDVSYVGRIRTDPGVPDGRGLALFVTGDNLRKGAATNAVQIAEYLVAKNLV",
    "response#3": "LKPLDELYGIKRVDVSTYQAVSGAGKAGMEELVQQMQDFFAFKLDESEIKAFAHQIALNVIPQIDVAMENGFTKEEMKMINETQKIMHKNFELAATCVRVPVLRSHSESLTVTFNEGTDVDVDEVRNALANFENVKVIDDLENKKYPMPIISTDTDFTYVGRIRKDVYSSNIVHFFNVADQVL"
  },
  {
    "reference": "MNQVEMTEFPVGKPQEALYGVASTPDGALWFTLAKGNAIGRLSPDGAVSRFPLPHADGQPTTITCGPDGRPWFTLSSANAIGRLAPDGALRMFELPRPASRPFGIAGGHDGCLWFAEMAGDRIGRITIDGDIEEYDLPVKGGYPSCMAAGRDGLMWFTLNQAGAVGSISATAAPRIFPLGAADAAPVGIASDAQGALWIAQAGNGAIARFDAGGRITEFPLHSRAARPHAIAADAAGNLWFTEWGANRIGRISEAGDLAGYELAAPGSEPHGIAIDPHGCVWAALETGRLVRLQASPRD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be optimized for magnesium ion binding.2. The designed protein should have a binding site that is specific to the target Mg(2+) ligand. The designed protein sequence is ",
    "response#1": "MLKAIKGHGYRDELVIPIIENTPYEYELTDSLSEAIAAYPKATAVLVRNHGIFVWGDSWISAKTQAESYHYLLK",
    "response#2": "MIYGVGVDIVNIERVERILSKNKEGFVKRVLSEYEQVLFAKKGDSSTYCAKRFSAKEAFAKALGIGIGKIVSFQDLTICNNKQGNPYFIFSAKLCLYLVDKNIKKAHLSLSDEKFNAMAFVILN",
    "response#3": "MIYGVGVDIVNIERVERILSKNKEGFVKRVLSEYEQVLFAKKGDSSTYCAKRFSAKEAFAKALGIGIGKIVSFQDLTICNNKQGNPYFIFSAKLCLYLVDKNIKKAHLSLSDEKFNAMAFVILK"
  },
  {
    "reference": "MPCRREEEEEAGEEAEGEEEEEDSFLLLQQSVALGSSGEVDRLVAQIGETLQLDAAQHSPASPCGPPGAPLRAPGPLAAAVPADKARSPAVPLLLPPALAETVGPAPPGVLRCALGDRGRVRGRAAPYCVAELATGPSALSPLPPQADLDGPPGAGKQGIPQPLSGPCRRGWLRGAAASRRLQQRRGSQPETRTGDDDPHRLLQQLVLSGNLIKEAVRRLHSRRLQLRAKLPQRPLLGPLSAPVHEPPSPRSPRAACSDPGASGRAQLRTGDGVLVPGS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be expressed specifically in the cytoplasm, cytosol2. For general function, the protein need meet that May play a role in tumor progression and collaborate with PIM1 and MYC in lymphomagenesis. The designed protein sequence is ",
    "response#1": "TLRAMETNPAERPSPYFLETTQGGRMTALVRASMIAFMDEFSRFYELADGTLQRAAYFLDRYLSVTPESDDVLQLRLVGATAVFLAAKYEDQYTLRKIDASMVAARCGYTSETRHKMVSCMETEILAALDYNLI",
    "response#2": "TLRAMETNPAERPSPYFLETTQGGRMTALVRASMIAFMDEFSRFYELADGTLQRAAYFLDRYLSVTPESDDVLQLRLVGATAVFLAAKYEDQYTLRKIDASMVAARCGYTSETRHKMVSCMETEILAALDYNLV",
    "response#3": "TLRAMETNPAERPSPYFLETTQGGRMTALVRASMIAFMDEFSRFYELADGTLQRAAYFLDRYLSVTPESDDVLQLRLVGATAVFLAAKYEDQYTLRKIDASMVAARCGYTSETRHKMVSCMETEILAALDYNLK"
  },
  {
    "reference": "MSTPAHPTAASSPPLHVIILAAGQGSRMKSRLPKVMHPLAGKPLLHHVIDAARALNAGGVHVVVGHGADAVRQATQADDLQWALQEQQLGTGHAVAQALPQVPDQARVLVLYGDVPLTDITTLEALTSLVNDETLALLTVNLSDPNGYGRIIRNDGEVTAIVEQKDANSQQLLIQEVNSGILAATAGNLKRWLPALSSENAQGEYYLTDIIAMAAGEQMTVAVAQPASPEEVQGINNRAQLAQLERHYQFSEAQRLMAAGVSLADPYRLDVRGDLQVGQDVTVDVGCVFEGTVVLGDDVVIGAHCVVRNSTLGAGTKVEPHSIIDGSQVAGPATIGPFARLRPGTRLAAGGKVGNFVETKNAIIGAGSKISHLSYIGDAELGEQVNVGAGTITCNYDGVNKHKTQIGDGTFIGSNTALVAPVTLGKNTTTGAGSTISKDVADGELAIGRARQRNITNWSRPEKK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The acetyl-CoA binding site should be stable and able to withstand changes in environmental conditions.2. The protein should be optimized for activity in UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The designed protein have MobA-like NTP transferase domains with specific sequence motifs that are crucial for its activity.4. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.5. The protein design must be able to perform UDP-N-acetylglucosamine diphosphorylase activity, magnesium ion binding, glucosamine-1-phosphate N-acetyltransferase activity in order to contribute to peptidoglycan biosynthetic process, lipid A biosynthetic process, regulation of cell shape, UDP-N-acetylglucosamine biosynthetic process. The designed protein sequence is ",
    "response#1": "MSNSAVILAAGEAKRMRSRQPKVLHPLCGRPLIAYALRTARALADRTVVVTGPNADDVRVAAGDGATFVEQRERLGTGHALGQARAACGDGPIVVLAADMPLLSSETLERLVAHHRGAGAAATVLTAVVDRPQGYGRILRQGGRVKRIVEDRDATDDQKKITEINTSVYCFEARRLWKALAEEAGVEGGGELYLTDVVDMVRARGRRVRPVVVEEAWETEGVNDRVQLAEMGRVLNDRVLEGWMRSGVTIVDPETTWIDSDVSIGRDATILPGTQLLGATSVGADARIGPDTTLTDTEVGDGAEVKRTEANLAQIGAGATVGPYSYLRPGTVLGAKGKIGGFVETKNARIGDGAKVPHLTYCGDATIGEGANIGAGTIFANYDGVAKHHTTIGAHSFVGSDSVLIAPVDVADGAYVAAGSALTGDVEPGQIAVARGRRADLAEAEAAFQARMRAQMMADGVTLIAPETVFFAHDTKIERDVVIEPNVVFGPGVVIEEDVVIHAHSHVEGAHLKRGAHAGPFARLRPGAELGEGSKVGNFVEIKKSQLAEGAKVSHLTYIGDASIGANANIGAGTITCNYDGYDKHRTVIGENAFIGSNTCLVAPVTVGAGAFTATGTIVTEDVPADALALARTPPIGGRGWEAPRNRPGTDAAAAARAAAEAGRAAADDDTAG",
    "response#2": "VVILAAGKGTRMRSSLPKVMQPLAGQPLLAHVLRVSRALGAIRPIVVYGHGGDVVQQAFTDEAIDWVLQAEQKGTGHAVQVALPVLESHGHTLILYGDVPLLSVATLQRLLATCADDSTVGVMTAVLDDATGYGRIIRDAAGRVCAIVEQKDADAAERAIREINSGIYAFDAATLREGLSRLGNDNAQGEVYLTDVLQMARADGARVRAVHARDTMEVEGVNDRVQLARLGAELNRRLLERWMLDGVTVQDPGVTYIDADVTIGMDTVILPNTSLRGRTVIGEGCEVGPNSVIQDSRIGDGCRVIASMLEGATLEAGVDVGPFSHLRPGAYLETGVHIGNYVEVKESRLGRGVLAGHFSYLGDASIGTGTNIGAGTITCNFDGTDKHPTKIGANAFIGCDTMLVAPVTVGEGAATGAGSVVTKDVPGHLLAVRADLAEAEAAFQARMRAQMMADGVTLIAPETVFFAHDTKIERDVVIEPNVVFGPGVVIEEDVVIHAHSHVEGAHLKRGAHAGPFARLRPGAELGEGSKVGNFVEIKKSQLAEGAKVSHLTYIGDASIGANANIGAGTITCNYDGYDKHRTVIGENAFIGSNTCLVAPVTVGAGAFTATGTIVTEDVPADALALARTPQRNIEGWVQRKRPGTPAAAAAEAARGRARGGEA",
    "response#3": "MAAGVIMAAGEGRRMVSKTPKPLHRVCGKEMIRYPVELLQALGLRRILVVVSPATAAPIRETLEESVQYVVQPEPNGTGDAAARALEALPEDIANLVLLGSDSPLIRSESVSRLVETHLADSNAMTMLTAPDMLCPDLGRVLRDETGKVSGLVEAAEWEGDPFEPAEVNAGVYCFARNWLQNTLPAVENSPSGEKYLTDIVEERDATPEQRGIREINSGIYAFDAATLRTSLGKLGTDNAQGEKYLTDVFEILRGEGERVGAHLAPDHWLTEGVNDRVQLAALGAELNRRTVERWQRADVTIGMDTVILPNTSLRGRTVIGEGCEVGPNSVVREGTVVLAAGQGSRMNSDLPKVLHKVGGAPLFAHALNAGAALEPERSVLIAGHGAEAVRAEASEWDEDLVVALQEQQLGTAHAVAQARDALEGAEGDVFILYGDTPFIRPETLSMMAAARAGGAAVVVLGFGRRATVKERGRVG"
  },
  {
    "reference": "MYENLLKTVTNLGSPKVLVVGDFMLDVYVYGDALRISPEAPVPILKVAKTEHRCGGAGSVAANLAALGAAPHCLGVIGDDRNSEILREKLTEAGVDISGLLEAANRPTVSKQRLIGLAQHRHRQQLIRIDEESAEPLSDEINEAVLSAYEEKLPEVDIVCLQDHNKGLLSNSLCSRMIRLANEADRKVLVDPSPASDYSKYIGATVITPNRKESSAVVGFEITNTETAAGAAEYLLRELKLKAVVITLDKEGAYLRTENTGLIVPTRARNVYDVTGAGDMVLATLAITLAAGCDYETAVQLSNIAGGIEVEKFGTATVTVEEIVDEIVSQHGGKGGKVHAADSLVSELDRQRRQRKTVVFTNGCFDVLHRGHIDYLAFCRRQGDVVVVGLNSDSSVRTIKGPERPIYGQDDRAVILAALETVDYISIFDEPDPLNLIKKVRPDVLVKGEDWAEKGVIGREFVESYGGRVVLARLVEGRSSTSTIEKMKSLEAKS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Ideal protein should have a binding affinity for key enzymes in the Nucleotide-sugar biosynthesis, ADP-L-glycero-beta-D-manno- heptose biosynthesis pathway to enhance the overall efficiency of the pathway.2. The designed protein must possess ATP binding, heptose 7-phosphate kinase activity to support ADP-L-glycero-beta-D-manno-heptose biosynthetic process under certain conditions.3. For general function, the protein need meet that Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7- phosphate at the C-1 position to selectively form D-glycero-beta-D- manno-heptose-1,7-bisphosphate.4. The designed protein should have a binding site that is specific to the target ATP ligand.5. The protein with the reaction ATP + D-glycero-beta-D-manno-heptose 7-phosphate = ADP + D-   glycero-beta-D-manno-heptose 1,7-bisphosphate + H(+), the active site must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MRVKGLRALVIGDLMLDEYLWGKAERISPEAPVQVVDVRREDMRLGGAGNVVNNLVAFGACVSICSVIGGDENGTVLRHAFTGKGVDVSGVFEDPMRMTSKKTRVLAANQQIVRIDRETREEISPEFEEMILAHVRDTRNIWDLFLISDYGKGLLTSRVLSGVIASAQERGIPVIVDPKGSDYRKYRGATIITPNRKEAEVASHIAITDAESLNRAADFLLAEGEYQALLITRSSEGMSLFLNAGEEVHIPTVAREVYDVTGAGDTVVATFGVGLACGLSFERAAQLANVAAGIVVGKVGTSTVSPEEIIGLVEEALASHAPARALVIGDLMLDEYLWGKAERISPEAPVQVVDVRREDMRLGGAGNVVNNLVAFGACVSICSVIGGDENGTVLRHAFTGKGVDVSGVFEDPMRMTSKKTRVLAANQQIVRIDRETREEISPEFEEMILAHVRDTRNIWDLFLISDYGKGLLTSRVLSGVIASAQERGIPVIVDPKGSDYRKYRGATIITPNRKEAEVASHIAITDAESLNRAADFLLAEGEYQALLITRSSEGMSLFLNAGEEVHIPTVAREVYDVTGAGDTVVATFGVGLACGLSFERAAQLANVAAGIVVGKVGTSTVSPEEILAERRLSHRGAGRGRIMSLDDLLVAEWRASGESIVFTNGCFDLLHIGHITLLEDCRRFGSKLIVGMNTDRSVSELKGPSRPIVGENERTKVMAALGSVDMVVLFDEETPLKLIRAMKPDVLVKGGDYRVETVVGHEDVMAAGGRVEIVPTVEGFSTSNIVKRM",
    "response#2": "MRVKGLRALVIGDLMLDEYLWGKAERISPEAPVQVVDVRREDMRLGGAGNVVNNLVAFGACVSICSVIGGDENGTVLRHAFTGKGVDVSGVFEDPMRMTSKKTRVLAANQQIVRIDRETREEISPEFEEMILAHVRDTRNIWDLFLISDYGKGLLTSRVLSGVIASAQERGIPVIVDPKGSDYRKYRGATIITPNRKEAEVASHIAITDAESLNRAADFLLAEGEYQALLITRSSEGMSLFLNAGEEVHIPTVAREVYDVTGAGDTVVATFGVGLACGLSFERAAQLANVAAGIVVGKVGTSTVSPEEIIGWLNPETADAKPFETAAFTGASGAPVVVDPKGRDFARYGAVDLIKPNASELAAATGLPVESDAEVEAALKALLHATTARAVVVTRAGKGMSLMRRGEAAKHFPGRAREVFDVSGAGDTGLAALGLALGAGTSLETAVEFAILASGVVVGKAGTAVVE",
    "response#3": "MRVKGLRALVIGDLMLDEYLWGKAERISPEAPVQVVDVRREDMRLGGAGNVVNNLVAFGACVSICSVIGGDENGTVLRHAFTGKGVDVSGVFEDPMRMTSKKTRVLAANQQIVRIDRETREEISPEFEEMILAHVRDTRNIWDLFLISDYGKGLLTSRVLSGVIASAQERGIPVIVDPKGSDYRKYRGATIITPNRKEAEVASHIAITDAESLNRAADFLLAEGEYQALLITRSSEGMSLFLNAGEEVHIPTVAREVYDVTGAGDTVVATFGVGLACGLSFERAAQLANVAAGIVVGKVGTSTVSPEEIIGFLEEGQSAESRILVVGDLILDRYVWGDAERISQEAPVILLREERQDTRLGGAANVARMLVGLDATVDLAGVIGADLDGRELSLGLQNSGIGTRAVFPDPSRPTTTKIRFMGRAQYRHPHQMLRVDREVRTPLPPSFADQILRAIRPRLSDYSAILVSDYAKGVCTPELLRLLIMAARQAGLPIVVDPAPGADYRHYQGATGVTPNRSETGKAVGREIRTVADALSAANTLVERFDLELVFVTLDRDGIVLGRRGTEPVHLPTRPREVCDITGAGDMVLATIGVGLAAGVDPVDLCRLANVAGGLEVEQIGVVPISRHDLLELLQRRERGMTGAVGFTNGCFDILHRGHVAYLAQARGWCDRLVVALNTDASVRRLKGEGRPINDLDSRAVVILDDARAHGEKIVFTNGCFDIIHAGHVGYLDQARKLGDRLVVAINSDASVSRLKGPARPINPLERRMAVLAGLEAVDWVTWYEDDTPERLLEILKPDILVKGGDYSKEQVVGWEIVEGYGGEVRALDFLDNCSTTAIVEKIR"
  },
  {
    "reference": "MASREYYSEGARAHQREFAKFLDSQDPLNRFRKEFIIPSKKDLKRKVLAVDEKEDPSDPRCIYFCGNSLGPQPRNTLKYIERYLRTWATKGVTGHFVPHEDELLPPFLHVEEAGSKLMAPVVGALQSEVAVMGTLTANIHLLMASFYRPTKENYKIILEGKAFPSDHFAVESQIQHHGLDPKDAMVLIEPEDPTNPILGTEQILRVIDDNASDAALILLPAIQFYTGQYFDIKKITAHAHSKGLLIGWDCAHAAGNVGLRLHDWDVDFAAWCTYKYLNSGPGGMAGLFVHERHGQVNPGKDSETVSFRPRLTGWWGSDKATRFLMNNKFFPQPGAAGYQLSNPSVLDMNAVVASLQLFNQTSMADVRKKSLDLTGYLEHLLLTLPFDFPNGKPFKIMTPANPAERGAQLSLLLTPGLLDSVLESLEQNGVVVDERKPDVIRVAPAPLFNTYTEVWEFCQIFLCACRDAVEKQRR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must have at least one Aminotransferase class V domain.2. The protein design should consider the endogenous proteins and enzymes present in the NAD(+) biosynthesis, Cofactor biosynthesis pathway to avoid interference with native metabolic processes.3. The designed protein should be able to compete with endogenous proteins or inhibitors for pyridoxal 5'-phosphate binding.4. A protein that can perform 3-hydroxykynureninase activity, pyridoxal phosphate binding, kynureninase activity in cytoplasm to support 'de novo' NAD biosynthetic process from tryptophan, L-kynurenine catabolic process.5. The protein should have a fast turnover rate for H2O + L-kynurenine = anthranilate + H(+) + L-alanine. The designed protein sequence is ",
    "response#1": "MSHKELKKLMAPIVGASPSEVCPMNTLTVNLHLLMVSFYRPNAKRYKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVKPKEGEYVLQTADIIKEIQENGDEIALVLFGGVNYFTGQLFDMPAITKAGHAVGAVVGFDLAHAAGNVPLSLHDWNVDFACWCSYKYQNAGPGGISGIFVHEKHFGNTEMPRFAV",
    "response#2": "MNHKELKKLMAPIVGASPSEVCPMNTLTVNLHLLMVSFYRPNAKRYKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVKPKEGEYVLQTADIIKEIQENGDEIALVLFGGVNYFTGQLFDMPAITKAGHAVGAVVGFDLAHAAGNVPLSLHDWNVDFACWCSYKYQNAGPGGISGIFVHEKHFGNTEMPRFAGGKAN",
    "response#3": "MNHKELKKLMAPIVGASPSEVCPMNTLTVNLHLLMVSFYRPNAKRYKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVKPKEGEYVLQTADIIKEIQENGDEIALVLFGGVNYFTGQLFDMPAITKAGHAVGAVVGFDLAHAAGNVPLSLHDWNVDFACWCSYKYQNAGPGGISGIFVHEKHFGNTEMPRFAGICNDRTKGGAHKELKKLMAPIVGASPSEVCPMNTLTVNLHLLMVSFYRPNAKRYKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVKPKEGEYVLQTADIIKEIQENGDEIALVLFGGVNYFTGQLFDMPAITKAGHAVGAVVGFDLAHAAGNVPLSLHDWNVDFACWCSYKYQNAGPGGISGIFVHEKHFGNTEMPRFAGICNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGVTCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCNDRTKGGATCND"
  },
  {
    "reference": "MSDNTTHFGYQEVSVTEKVKKVGAVFDSVASKYDIMNDVMSMGVHRLWKWFALEVCAINEGERVLDIASGTGDLAAKHAERTGNEGLVIASDINNSMLNVGRDRLTDRGIIGNIQYVQADAEQLPFPDNYFDCITIAFGLRNVTNKDTALASMQRVLKPGGRLLVLEFSKPHSFLKGIYDTYSFKLLPLFGKVIAKDEDSYRYLAESIRMHPDQETLKTMALDAGFDQCDYMNMSGGIVAIHRAYKF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Ideal protein should have a binding affinity for key enzymes in the Quinol/quinone metabolism, menaquinone biosynthesis, menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2 pathway to enhance the overall efficiency of the pathway.2. The protein should have a low Km value for a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine.3. A protein with S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity, S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity, S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity, demethylmenaquinone methyltransferase activity, 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity is needed to enhance aerobic respiration, ubiquinone biosynthetic process, methylation, menaquinone biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "MTAGLSGPAGRTSPTDRPSTAARPRTARRASAAEPAAGAGPAHVVPRPATGSGHAPASGSGHAPASGSGHASSSGSAHASGSAAGSESAAGADVAPAVSASPAHASASEPASGAGHAAASGSGHAPASGSGHAPASGSGHAAASGSRMAPAPRMGAAPAPDTRPAPDPGSESGPGSAPGPASGSGSGSAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPVSGSGHAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPGSGSGQAAAPRMAPGTASGPASAPASGSGQAPAAGSGHAAGSGPGPAPASGSGHAAASGSGHAPAPRMGAAPAPDTRPAPDSGSESRPGSAPDPASGSGHAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPAPRMGAAPAPDTRPAPDSGSESRPGSAPDPASGSGHAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPASGSGHAPGSGSGQAAAPRMAPGTASGPASAPASGSGHAPASGSGHAAGSGPGPAPASGSGHAPASGSGHAPAPRMGAAPAPD",
    "response#2": "MTIAAESGTVSFGFRDVAAAEKQGRVGQVFASVAARYDTLNRILSFGVDQGWKRGFVERLAPPPGGRALDLAGGTGDIAFRLADAGHQVTGADFSAAMLAVGRKRALDRGLADGLRWVEQDAQTLGFPDRSFDAYTIAFGIRNVPRIDAALAEARRVLKPGGRFLCLEFSHVTVPGLDRLYDAYSFRVLPRLGQLVAGDADSYRYLAESIRVHPDQKGFAAMIGAAGFARVRHRPLTGGIAAIHSGWKI",
    "response#3": "MNNENSNQQTFGYQNVPVQEKVNKVAEVFHSVASNYDTLNRILSFGVDQGWRRFAVEQAGLSKGGRVLDIAGGTGDLARQFSRLVGDEGEVVLADINASMLKVGRDKLLDKGVAGNIRYVQANAECLPFTDNYFDCVTIAFGLRNVTNKDKALASMWRVLKPGGRLLVLEFSKPTNPLLRQVYDQYSFKLLPLMGKLVAKDAESYRYLAESIRMHPDQETLKGMMQDAGFEQVEYFNLTGGIVALHRGYKF"
  },
  {
    "reference": "MRVRGIQRNCQQWWIWGLLGFWMFMICNVVGNLWVTVYYGVPVWKEASPTLFCASDAKAYDTEVHNVWATYACVPTDPNPQELVMENVTENFNMWKNDMVEQMHQDIISLWDEGLKPCVKLTPLCVTLHCSSNVTSNSNVTSNSTDASKVNKDIIGQMKNCSFNITTEVRDKNKKAYALFYRLDIVPLTADNSSGEYRLINCNTSTITQACPKVSFDPIPLHYCAPAGYAILKCNDKTFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSIAEDKTIIRSENLTNNAKIIIVQLNEPVEINCTRPNNNTRQSIRIGPGQTFYATGEIIGDIRQAHCNISGDRWNKTLESVKERLQKQFPNKTILFAPSSGGDLEITTHSFNCRGEFFYCNTSKLFNTTGLSDNATLTIPCRIKQIINMWQEVGRAMYAPPIKGIITCRSNITGLLLTRDGGTNNTNNTETFRPGGGDMRDNWRSELYKYKVVEIKPLGVAPTGAKRAVVGREKRAALGALFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLRTRVLAIERYLQDQQLLGIWGCSGKLICPTTVPWNSSWSNKSQEEIWENMTWMQWDREISNYTGTIYRLLEVSQNQQEKNEQDLLALDKWKDLWSWFSITQWLWYIKIFIMIVGGLIGLRIIFAVLSLVNRVRQGYSPLSFQTLTPNPREPDRLRGIEEEGGEQDRDRSIRLVNGFLAIAWDDIRSLCLFSYHRLRDLIL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should contain a Helical membrane-spanning region.2. Include a signal peptide as an essential component of the protein.3. The presence of Retroviral envelope protein GP41-like, Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains is critical for the interaction of the designed protein with its intended targets.4. The protein design should include a YXXL motif; contains endocytosis signal motif to ensure proper function.5. For general function, the protein need meet that The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; Transmembrane protein gp41: Acts as a class I viral fusion protein; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain The designed protein sequence is ",
    "response#1": "LEMKNCSFNMTTEIRDKKQKVFSLFYKLDIVKINGNNNSNSSGEYRLINCNTSAITQACPKVSFQPIPIHYCAPAGFAILKCKDEEFNGTGPCSNVSIVQCTHGIRPVVSTQLLLNGSLAKGEVKIRSENISDNAKTIIVQFNQSVAINCTRPNNNTRRGIGIGPGQTFYATDDIIGDIRQAHCNVSRAQWNNTLRQVVTQLKKYWNKTITFANSSGGDLEITTHSFNCRGEFFYCNTSDLFNNIWIGNETTETNETETNSSKPITLQCRIKQIINMWQRVGQAIYAPPIQGKLECNSNITGLLLTRDGGKNNDSKEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRAQRRVVKREKRXLGAXALXGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLHEIWDNMTWMEWEREINNYTREIYTLIEKSQNQQEKNEQELLELDQWASLWNWFDITKWLWYIKIFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTXLPTQRGPDRPEGIEEEGGERDRDGSGPLVDGLLAIIWIDLRSLCLFSYHRLRDLL",
    "response#2": "MRVKGTQMNWPNLWKWGTLILGLVIICSASNNLWVTVYYGVPVWKEANTTLFCASDARGYDTEVHNVWATHACVPTDPNPQEVVLENVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNLRNDTNTLWVTVYYGVPVWKEATTTLFCASDAKAYDKEAHNVWATHACVPTDPDPQEMGLENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNANVTTLTNTTGANLTSSGEMKNCSFNTSTDIQDKKQKNYALFYVMDITPIDNNSTSYKLVSCNTSVITQACPKISFEPIPIHYCAPAGFAILKCRDKNFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEDEVIIRSENITNNAKTIIVQLNETVQINCTRPANNTRKSIRIGPGSTFYAHGDIIGDIRQAHCNISRKQWNDTLKMIVKKLGEQFKNKTIVFNHSSGGDPEIVMHSFNCGGEFFYCNTAELFNSTWDNGTRNDTDRSNSTEITLQCRIKQIINMWQEVGRAMYAPPIAGQINCTSNITGLLLTRDGGTDSNNTEIFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTRARRRVVQREKRAVGIGAMIFGFLGAAGSTMGAASITLTAQARQLLSGIVQQQSNLLRAIEVQQHMLQLTVWGIKQLQARVLAIERYLTDQQLLGIWGCSGKLICTTAVPWNSSWSNISQEDIWNNMTWMEWDRQISNYTGTIYRLLEDSQNQQEKNEKDLLALDSWKNLWSWFDITNWLWYIKIFIMIVGGLIGLRIIFAVIAIVNRVRQGYSPLSFQT",
    "response#3": "MRVMEIRKNYQHLWRWGTMLLGMLMICSAAENLWVTVYYGVPVWKEANTTLFCASDARGYDTEVHNVWATHACVPTDPNPQEVVLENVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNLRNDTNSVNNSASNNTNVDNAXADIKNCSFNITTGIGNKMKKEYAFFNILDVTPIEGDNTSYILTSCNTSVITQACPKVNFDPIPIHYCAPAGFAILKCNDKEFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIVIKSSNITNNARNIIVQLNETVRINCTRPNNNTRKSIHIGPGSAWYTTGEIIGNIRQAHCNISEKDWEKTLRRVAIKLREQFNKTIIFNQSLGGDPEIVMHIFNCRGEFFYCNTTRLFNSTWKGNETATNNGTITLPCXIKQFINRWQGVGXAMYAPPISGNISCSSDITGLLLTRDGGYNNETDSEIFRPAGGDMKDNWRSELYKYKVVRIEPLGIAPSRAKRRVVQREKRAALGALFLGFLGAAGSTMGAASLTLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTTVPWNVSWSKNKTLDEIWNNMTWMEWEREIDNYTEVIYTLIQNSQNQQEKNEQELLELDKWNSLWNWFNITQWLWYIKIFIIIVGGLVGLRIVFAVLAIVNRVRQGYSPLSFQTRLPTPRGPDRPEGIEEE"
  },
  {
    "reference": "MQTLVGKRIVVGITGGIAAYKSAELVRELIRAGAEVQVVMTEAAQEFITPLTLQALSGHPVHRSLLDEEAEMGMGHIELARWADLLLVAPATADFIARLASGMGNDLLTTLCLATRAPLALAPAMNEKMWLNPVTQANVKKLTDLQPDTLWFGPDAGQQACGDVGPGRMLEPVGLAELTARAFAPGFLSGRKVVITAGPTREALDPVRYLSNHSSGKMGFALAAAAAEAGADVTLIAGPVQLATPPRVKRLDVTTALEMNDAAQEVLEAVDLFIATAAVADYRPADVPTQKIKKAEGQSGLTLELIENPDIVAGVAASTQRPTQVIAFAAETENLENHARAKLEKKAVDWVVANDVSRSDIGFGSDLNDILLVSAYGSEPFGPASKAQVARWLIEQFATQLPPIQDIE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should interact with enzymes and substrates within the coenzyme A biosynthesis pathway to enhance or regulate its activity.2. The protein design must be able to perform metal ion binding, FMN binding in order to contribute to coenzyme A biosynthetic process.3. The Proton donor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.4. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A5. The designed protein should contain one or more Flavoprotein domains that are essential for its function. The designed protein sequence is ",
    "response#1": "LRDAGSDVRVVMTRGARAFVTPLTFQAVSGNPVRTELLDPDAEAGMGHIELARWADRILVAPATANFMARFAQGLADDLLSTLCLATTAPVALAPAMNRQMWAHPATQANCAVLEERGVRLIGPESGSQACGETGPGRMTEPAELVAAVEAALTDPRLTGRKVVVSAGGTREFLDPVRYLGNRSSGRQGYALAKTAAARGATVTLVAANTALPDPAGVDVVRVVSTNDLRDAVFAAATDADAVVMAAAPADFRPADYVGTKIKKTDDTSAPTVTLVQNPDILAGLVRSRTGAGPVIVGFAAETGDSTGTVLDHGRAKLARKGCDLLVVNDVSGGKAFESAENAAVIALGPDGGAEVLARADKLTLARALVERIAGLLG",
    "response#2": "KNILLAVCGGIAVYKVVDVASRLRKLGANVHVVMTKEATNFVTPLTFQEISGNPVNVSMWDKVTNWNVEHIALANLADLVLIAPATANIIGKIANGIADDMLSTVIMATKAPVFVAPAMNSNMYQNPITQKNINTLREYNYHIIEPASGHLACGVNGIGRLPEPITLVETIKEHIARGDWKNKRFRVY",
    "response#3": "RILLVIGGGIAAYKSLELIRLLKKAGAGVVPVLTAGGAEFVTPLTASALAGETAHTLLFDLTREAEMGHIELSRSADLVVVAPATADLIGKIAGGLADDLASTLLLATDKRVLLAPAMNVRMWLHPSVQRNVAALAADGVLTVGPNHGDMACGEFGPGRMAEPAEIVAAVAALRDAGSDVRVVMTRGARAFVTPLTFQAVSGNPVRTELLDPDAEAGMGHIELARWADRILVAPATANFMARFAQGLADDLLSTLCLATTAPVALAPAMNRQMWAHPATQANCAVLEERGVRLIGPESGSQACGETGPGRMTEPAELVAAALAAVHA"
  },
  {
    "reference": "MNDSLKAQCIAEFLGTGLFLFFGIGCLSALKVAGASLGLWEICIIWGLGISLAVYLTAGISGAHLNPAITIALWLFACFPGRKVLPYTVAQVAGAFGGALLAYLLYGSLFTEYESAHQMVRGSLESLHLASIFSTYPAAALSVWQAALVEVVITSILMGMIMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKLFAWMAGWGDVAMTGGRDIPYFIVPIVAPIIGACAGAAIYRYLIGKNLPCNTCKLDENES",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must be optimized for channel activity.2. The protein design must feature the NPA motif.3. The protein must exhibit the following characteristics: Probably facilitates diffusion of 1,2-propanediol (1,2-PD) into the cell.. The designed protein sequence is ",
    "response#1": "TTLKMVIPDSQNALPPPVPKTYEVRVLHAPTGSGKTTKIPMGYVSEGYNVLVLNPSVATTLSMGPYMQKAFGIAPSIHTGETSVGTGTKLTYATYGKVAAMDTGLLQGADVIICDECHDTTSTTLLGIGHVLMKAEACGTKLVLLATATPPGCVSTPHPQISEVELGNSGEVPFYGKKLELANYLKGRHLR",
    "response#2": "VLHAPTGSGKTTKIPMGYVSEGYNVLVLNPSVATTLSMGPYMQKAFGIAPSIHTGETSVGTGTKLTYATYGKVAAMDTGLLQGADVIICDECHDTTSTTLLGIGHVLMKAEACGTKLVLLATATPR",
    "response#3": "DKLMGRTSGYDARIRPNFKGPPVNVTCNIFINSFGSVTETTMDYRVNIFLRQKWNDPRLAYSEYPDSSLDLDPSMLDSIWKPDLFFANEKGANFHDVTTDNKLLRIFKDGTVLYSIRLTLILSCPMDLKNFPMDVQTCTMQLESFGYTMNDLIFEWLDKGPVQVADGLTLPQFLIRDEKDLGYCTKHYNTGKFTCIEVKFHLERQN"
  },
  {
    "reference": "MRAAEFIPDVEKALKNRGEPSYRLGQAYGALCGGLIRDWEEATALPKGLRAALSAEAPAAVLELTRASRATDGTRKYLFKTHDRHLIETVMIPEKDRRTVCISTQVGCSMGCTFCATGLLGIKRNLKAREIAEQVFVAARDVAPERITNVVVMGMGEPFLNYKETLLALKVLNDKAGFNLAARSIAASTSGLVDKIRRFADEPEQFHLAISLHTPFEEQRKEIMPVASRHSIPELMDAARYYVEKTHRKLFFEYTLLAGVNDQPRHVEALSSLLDHPLYHLNLLRFNWTDTGFAATPKREAKEFLRYARELGLPATLRPSRGQDIDAACGQLAAKDLQARPARTVIPLSRA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.2. The designed protein must be able to perform rRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding, rRNA binding under specific conditions. The designed protein sequence is ",
    "response#1": "PKFGLSVCITTQVGCNMGCSFCASGLLTKTRDLSSGEIVEQIMKVQLYLDKMKHDERVSHVVVMGIGEPFDNFLNVVDSLRIITDDKGLAIGGRHITVSTSGLVDKIIEFSDTNLKINLAISLHAPTNELRTRLMKINRAFPIEKIMQAVDYYLEKTKRRTTMEYILLKDLNDRREHALQLADLIGARRDLTNVNLIPYNPVDEHTQYQRSEHQTMLAFYDTLKKQGINCCIG",
    "response#2": "ASDRHTLCVSTQVGCAYGCKFCASGLDGWKRNLQPNEIVEQVQAVERWGAEQSRAESRESSAPLTRPADTLSPSDGERDGVRGARPSTLDPRLVDNLVIMGMGEPLANYDNLLKALRILNAPWGGGIGARKITISTSGLAPQIRKLADEPEQFRLAISLHGATDEVRAKIMPVNRKFPLKELTAALDYYQSNKARMLTFEYILIAGVNDSLEQTKPLAALAKRLHAKVNLIPYNTVEGLSWSRPDEETCENFLAALEKQKVIATLRREKGHA",
    "response#3": "SSQVGCTLRCTFCHTGTQDLVRNLSSAEIVGQVMAARDALGEWPGQPSENQRQMTNIVLMGMGEPLFNFDNVSKALHIIMDGEGISISKRRITLSTSGVVPMIGRCGAELGVNLAISLHATNDEVRSRIMPINRKYPIASLMAACKAYPGLSNARRITFEYLMLKGINDSQD"
  },
  {
    "reference": "MELKGKRVLVVGLGESGLAMAKWLHRQGALVRVADSRDNPPNIDALQRVAPGAELVAGAFAEATFAGADFVALSPGVPKATPEIAALEIPLISEIELFADGVREQVPNSQIIAITGSNGKTTTTALTAHLLNGAGVPAIACGNISPSALDALMDAQDAGALPQVWVVELSSFQLETTHHLNAAAATVLNVSEDHLDRYEGSLANYAAAKSRVFQGKGVMVLNRDDDWSMANGRCGRKMVTFGLNAAPRGVDYGYADGAIWRGKDKLVAIDALKLSGLHNAANAMAALALCEAIGVDPLRLIEPLKGFSGLPHRVETVAEIGGVLYVDDSKGTNVGATLAAIEGMGRKVAIVLGGDGKGQDFSPLKPALEKHGRAVALIGRDAAAIGMALEGSGVPTRILGDMEAAVLWLAAQAQAGDCVLLSPACASLDMYRNYAHRAQAFIDAVEGLKS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that can perform ATP binding, UDP-N-acetylmuramoylalanine-D-glutamate ligase activity in cytoplasm to support peptidoglycan biosynthetic process, cell cycle, regulation of cell shape, cell division.2. A protein that can modulate the overall flux of metabolites through the peptidoglycan biosynthesis pathway, enhancing or suppressing its activity as needed.3. The ATP binding site should be located in a region of the protein that is accessible to the ligand.4. The protein must exhibit the following characteristics: Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).; Cell wall formation. The designed protein sequence is ",
    "response#1": "ITGSNGKSTVTAMCGDMCRMAGLTVCVAGNIGLPVLDALHEIEQGAAPLPQVWVLELSSFQLETTSSLDATAAAVLNLSEDHMDRYPDMAAYAAAKARIFSGNGVQVLNRDDPRTLAMAIPGRHVVSFGLDRCPTDENFGLCEDELCLGGDMLMPLSVLAVPGLHNAANALAALAILTRLGIPLDAALPALGHFSGVRHRCEWVADKDGVAYINDSKATNVGATQAALAGLGPVTSGGIVLICGGQGKGQDFTPLAPAVKEWVSTLIIIGEDGPKLKEALAGGVMALSAETMEEAVKLAAEKSSPGDLVLLSPACSFDMFENYEHRADIFTAAVAALPATGGR",
    "response#2": "IGGTHGKTTTTSLVAAILDKAGLDPTVINGGIINAYGTNARLGGGEWMVVEADESDGTFVKLPATIAIVTNIDPEHLDYYGSFDAAKDAFLAFVENVPFYGAAVMCIDHPEVQALIGRVRDRRIVTYGTNPQADIRATNARVEDGFNVFDVAITDRKTGTAREMTGVRLAMHGTHNMLNSLAAIGVATASALGISNDDFVSALAGFAGVKHRLEPICTWSGIQFINDSKATNYDAAETGLVAVPAPVILIAGGQAKQGDDRRWLEMIQTKAAAVLLIGAAANLFAQRLQEVGYDRYEIVETMANAVPRAAELAKKYSANIVLLSPACSSLEQFKSYEHRGDMFRHAVEQR",
    "response#3": "IGGTHGKTTTTSLVAAILDKAGLDPTVINGGIINAYGTNARLGGGEWMVVEADESDGTFVKLPATIAIVTNIDPEHLDYYGSFDAAKDAFLAFVENVPFYGAAVMCIDHPEVQALIGRVRDRRIVTYGTNPQADIRATNARVEDGFNVFDVAITDRKTGTAREMTGVRLAMHGTHNMLNSLAAIGVARELGIATDAIAAGLKGFSGVKHRSQTVGEKGGVRFVNDSKATNVDSAAKALQAFPKIRWIAGGLGKDGGIAALQPHLGSVVKAYLIGHSARDFALQIGATDHEICETMERAVARAAEEAQPGEVVLLAPANASWDMYPTFEVRGEDFANAVAQL"
  },
  {
    "reference": "MALIALGVTGGIGAYKAVEVCRGLQKAGHDVVAVMTRSAARFVGPVTFEAITRRPVITSQWRPGANADIEHIAIADEIALLLVAPCTANTLGKFASGIADDFLSSLYLATRAPVLLAPAMNSNMLAHDAVQQNLRVLTGRGVRMVEPGEGYLACGWIGKGRLAEPAEVVSAALDMLAPPASILRGRRVLVTAGPTYEDIDAVRYVGNRSSGRMGFALADEARRRGATVTLIAGPTSIEPPVVAETVRVRSAREMHSAVMAAAQSADVVIMAAAVADYTAATRAEDKIPKSEGPMTLVLERTPDILADLARLPSRAASATPVLVGFAAETGNAVAKARAKRTRKNVDLIVANDVSAPGAGFDVPTNEVTIVSEAREQHVALASKERVAQAILDQIEIILTRTRTAVPAS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.2. A protein should be able to manipulate the activity of enzymes in the CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis pathway, either by activating or inhibiting their function.3. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A4. The Proton donor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.5. The CTP binding site should be located in a region of the protein that is accessible to the ligand.6. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal domain.7. The designed protein must possess phosphopantothenoylcysteine decarboxylase activity, FMN binding, metal ion binding to support pantothenate catabolic process, coenzyme A biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "MNVLIGVTGGVAAYKVCEVVSTLAKSGASVRVMMTARSQDFVSALTFAALARNAVYTDVEFWSAQQGRPLHIELGEWADVLAITPLTANTLGKLAHGLADNLLTNTVLASVCPVLLAPAMNTDMWQQQSVQRNWEGLRQDARYHTVGPGAGRLACDRIGTGRMAEPVEIVAQIDDALAPACLTGKRLLISTGSTQEYIDPVRYIGNPATGRMGIALALAGLHRGASVTLVHGPMSAELKATVPGDVRTIGVTTAAEMEQVLLAETAIADWIIMAAAVADVRPTTTADFKLPKKDLPSSLPLSAVPDIVAKLSAGRTAGQKVIGFAAQTGDVVAPALDKLKRKQLDVILANPIDQPGSGFGSLDNEGVLAGLAAHRPLTGKRLLISTGSTQEYIDPVRYIGNPATGRMGIALALAGLHRGASVTLVHGPMSAELKATVPGDVRTIGVTTAAEMEQVLLAETAIADWIIMAAAVADVRPTTTADFKLPKKDLPSSLPLSAVPDIVAKLSAGRTAGQKVIGFAAQTGDVVAPALDKLKRKQLDVILANPIDQPGSGFGSLDNEGVLAGLAAHRPLTGKRLLISTGSTQEYIDPVRYIGNPATGRMGIALALAGLHRGASVTLVHGPMSAELKATVPGDVRTIGVTTAAEMEQVLLAETAIADWIIMAAAVADVRPTTTADFKLPKKDLPSSLPLSAVPDIVAKLSAGRTAGQKVIGFAAQTGDVVAPALDKLKRKQLDVILANPIDQPGSGFGSLDNEGV",
    "response#2": "MNVLIGVTGGVAAYKVCEVVSTLAKSGASVRVMMTARSQDFVSALTFAALARNAVYTDVEFWSAQQGRPLHIELGEWADVLAITPLTANTLGKLAHGLADNLLTNTVLASVCPVLLAPAMNTDMWQQQSVQRNWEGLRQDARYHTVGPGAGRLACDRIGTGRMAEPVEIVAQIDAALNPHEKPQHLSVMITAGPTREALDPVRFISNQSSGKMGFAIAQAAAARGAKVTLVAGPVTLPTPAGVNRIDVVSALEMQQTVQNLAAQQNIFISCAAVADYRAEQVSDEKIKKQGDEITLKLVKNPDIVAGVASMTKKRPFVVGFAAETQNVEEYARQKLARKNLDLICANDVSLAEHGFNSDTNALHLYWQQGAKTLQLDATTKTALAKQLLRHIAQTHR",
    "response#3": "MNVLIGVTGGVAAYKVCEVVSTLAKSGASVRVMMTARSQDFVSALTFAALARNAVYTDVEFWSAQQGRPLHIELGEWADVLAITPLTANTLGKLAHGLADNLLTNTVLASVCPVLLAPAMNTDMWQQQSVQRNWEGLRQDARYHTVGPGAGRLACDRIGTGRMAEPVEIVAQIDAALGGGALLEGRRIVVTAGGTREALDPVRFIGNNSSGKQGVAFAAAAAARGADVTLIAANLEVHAPGGVTLVPVVSAEELAREVFAAARGADAVVMAAAVADYRPAERSSRKLKKEALGDGLTIRLVKNRDILSSLVLEKSVAGPIIGFAAETAEGEELLDLGRQKMARKKCDLLVINSVGGDEGFGSSTNTVTVLTRGSEVEILPLMSKDELARVILERVVALLHE"
  },
  {
    "reference": "MFRWAIIFAVIALLASFLGFGGVAGLSANFAYILLALAVILFIVAFVSRRM",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "M",
    "response#2": "MPIGVPKVPFR",
    "response#3": "VDNK"
  },
  {
    "reference": "MEFAAENEGKSGGGLHSVAEGVRLSPEPGREGVRDLAGAEEFGGGEEGTGLTGIKEIGDGEEGSGQRPEEIPMDLTVVKQEIIDWPGTEGRLAGQWVEQEVEDRPEVKDENAGVLEVKQETDSSLVVKEAKVGEPEVKEEKVKEEVMDWSEVKEEKDNLEIKQEEKFVGQCIKEELMHGECVKEEKDFLKKEIVDDTKVKEEPPINHPVGCKRKLAMSRCETCGTEEAKYRCPRCMRYSCSLPCVKKHKAELTCNGVRDKTAYISIQQFTEMNLLSDYRFLEDVARTADHISRDAFLKRPISNKYMYFMKNRARRQGINLKLLPNGFTKRKENSTFFDKKKQQFCWHVKLQFPQSQAEYIEKRVPDDKTINEILKPYIDPEKSDPVIRQRLKAYIRSQTGVQILMKIEYMQQNLVRYYELDPYKSLLDNLRNKVIIEYPTLHVVLKGSNNDMKVLHQVKSESTKNVGNEN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding.2. The ideal protein would be one that plays a critical role in maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA), snoRNA localization through identical protein binding within pre-snoRNP complex, nucleus. The designed protein sequence is ",
    "response#1": "ILKPLFDDKDINDKRVKCNDVSGDSSVPVLHCNEVIEKLSALQDNDITSFDDIFFKELKIKEEHYTEKDRYISDPGFLNKVLDLIFGKFSGNDYPKTLTFLLTHPLFPLSRTRNLLSLLRDQPRLFKQAIVTCPNLPLNELLEELFSIRNRELLLDISFRILQDFTRDSIKQEMKKLSKLDVQNFIEFITSGGEDSSPECFNPSQSTQLFQLLSLVLDSIGLFSLEGALLENLTLYIDKQVEIAERNTELWNLIDTKGFQHGFASSTFDNGTSQKRALPTYTMEYLDC",
    "response#2": "GQQSSDIIQKLQKDLFMLKKPEAIQYNKKNAFRPMESAESIEFLGEKSNSSLFTFGSHSKKREHNLIMGRLFDNAVFDMIEMGISNFKSIKEFGADGSAVGNKPMFIFQGNEFENNETVKKVGNLFLDMFRGRLVEYINLVGLDHVVIMTAVDDKILFRHYTTIFSPSGSKIPKVDLKEVGPSFDITIRR",
    "response#3": "LEFLVPIILEIAAKLLASGSTDCQIAAHTILIVFTAALPLKKDIVVAAIETILANLKDKKAEQSAFWAVIKISQTLKGQQAIEHLPANIYKIFDKHYTWVRVLDLLTEEPSIKCDKFMTSYIRD"
  },
  {
    "reference": "MEERYNPEKVEPLWQAYWNQHQTFKATEDGSKPKYYLLEMFPYPSGKIHMGHVRNYTIGDVVVRYKRMKGFNVLHPMGWDAFGMPAENAAIDNNTHPAAWTYENIRTMRRQLKRMGFSYDWDREIATCRPEYYRWEQWLFLKMLDKDMVYRKESYVNWCDHCQTVLANEQVEQDMCWRCGKPVQQKKLWQWFFRITDFAEDLLVHCDKLPGWPDNVTLMQKNWIGKSQGSEIRFPIQGIDENIPVFTTRPDTLFGSTFMCLAPEHPLVETLSRGTDQAAAVTEFTTRVLNQERSSIALEKYEKEGVFTGAWCINPVTREKMPIYTANFALMEYGTGAVMSVPAHDQRDFDFARKYGLPIKVVIQPPGEPLDPATMIEAYTGQGTLADSGEFSGLGNLEAMGAITRWLENKGLGKTTVSFRLRDWGISRQRYWGTPIPVIHCPDCGIVPVKEETLPVVLPEDAALLDNGGSPLATLDGFARVNCPVCNRADARRETDTMDTFVESSWYFARYCSPRYDKGMFDPAAVAYWMPVDQYIGGVEHAILHLLYSRYFMRVLNTLGLIDFKEPFERLLTQGMVCKETLTCPEHGFIYPDDAETVNDKVICKRCNSDVEVGRVIKMSKSKKNVIDPNALLDQYGADITRLFCLFAAPPEKDLEWNDDGVEGCNRFINRVWRLADRCKSTYIDLLPYSGVVADLKGEPKKLFIKANQTIKKVTDDIEESFHFNTAISAVMELVNAMYSADLDPDQTDMGEVALFCIENIVLLLSPI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.2. The designed protein must be optimized for ATP binding, aminoacyl-tRNA editing activity. The designed protein sequence is ",
    "response#1": "MKTIIVGIDGSHAAITAALWGVDEAISRAVPLRLVSVIKPTHPSPDDYDRDLAHAERSLREAQSAVEAAGKLVKIETDIPRGPAGPVLVEASRDAEMICVGSVGIGRYASSILGSTATELAEKAHCPVAVML",
    "response#2": "FEGPDGAGKTTVLEAVLPLLREKLSQDILTTREPGGVTISEEIRHIILDVKHTQMDKKTELLLYMAARRQHLVEKVLPALEEGKIVLMDRFIDSSVAYQGSGRGLDKSHIKWLNDYATDSHKPDLTLYFDVPSEVGLERIQKSVQREVNRLDLEQLDMHQRVRQGYLELADSEPNRIVTIDASQQLDEVL",
    "response#3": "DRWAFKHPEKTAIIYEADEPGEGREISYAELLREVCSIANVLKSFGVKKGDTVSVYLPMTWQAVAAFLACARIGAIHSVVFAGFSAEALRDRMQDCKSRVLITSDEGRRGGKAIATKAIADAALKECPAVEKVLVLKRTGNPVPWTEGRDVWWHEAVARVPRYCPPEVMASEDPLFILYTSGSTGKPKGVVHTTGGYLLCAALTVKYVFDVHPDDRFACMADVGWITGHTYIVYGPLAIGATTTVFESTPVYPTPSRYWETVEKYKLTQFYSAPTAIRLLRRLGHEHVNKHDLSSLRVLGSVGEPINPEAWHWYNEHVGKTECAIVDTFWQTETGSIVVTPFPGAIETKPGAATVPFFGIEPAILEPTTGKVLEGNDVEGVLTIAHPR"
  },
  {
    "reference": "MFVARRLSKNITQISKTAVKTSVRAVPVRGFKTTSVNQAAAPAPKTSQEKEYFEKNKSLLDKESNEESTEVDYSKLNENQGVVHQVIGAVVDVYFPHGKLPYINDALIVDDFQAENVEKVIEDLNNPSLKGKIDFNNVPISNIKLVLEVAQHLGDGIVRCVALDITDGLGRGALVLNTGSPLMVPVGQATLGRIMNVIGEPIDGCGPIPATEKRPIWRAPPPFADLAPSASILETGIKVIDLLAPYSRGGKIGLFGGAGVGKTVLIQELINNIAKAHGGFSVFTGVGERTREGNDLYHEMVEAGVIKKEGPGSKVALVFGQMNEPPGARARVTLTGLTVAEYFRDAEGQDVLLFIDNIFRFTQAGSEMSALLGRIPSAVGYQPTLATDMGCMQERIATTKKGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRAISELGIYPCVDPLDSTSLMMDPNIIGVEHYKVATDVQKILQEYKSLQDIIAILGMDDLSEDQKATVFRARKIQRFLSQPFEVAHAFTNMEGRFVKLSDSIKAFKGILEGKYDHLPEAAFYMVGGIEDVEIKAAKLLESAGKEEKKTTTTSTTGQVKEETVREKVTRLVDEAYNAKVKKLKEYGASTAHLEELRAQYEKNFESEISQLEVLLKK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.2. For general function, the protein need meet that Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain; Subunits alpha and beta form the catalytic core in F(1); Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits .3. The ideal protein would be one that plays a critical role in proton motive force-driven ATP synthesis, proton motive force-driven mitochondrial ATP synthesis through ATP binding within mitochondrion, mitochondrial proton-transporting ATP synthase, catalytic core, mitochondrial proton-transporting ATP synthase complex, proton-transporting ATP synthase complex, catalytic core F(1).4. The protein should have a fast turnover rate for ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate. The designed protein sequence is ",
    "response#1": "RGGKIGLFGGAGVGKTVLIMELINNVAKAHGGFSVFAGVGERTREGNDLYREMIESGVIKLGEKQSESKCALVYGQMNEPPGARARVGLTGLTVAEYFRDAEGQDVLLFIDNIFRFTQANSEVSALLGRIPSAVGYQPTLASDLGALQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRQISELGIYPAVDPLDSTSRMLSPHILGEEHYNTARGVQKVLQNYKNLQDIIAILGMDELSEDDKLTVARARKIQRFLSQPSRMLSPHQVKAMKQVAGQIKLFLAQYREVASFAQFGSDLDAGTRATLDRGLRLTELLKQPQYSPLAVEEQVPLIYCGVKGYLDKIPVDRVVEFEHKFIPYLRSSGAEIMEAIRKEGVLSKTTEDSLKAVIKEYMDRF",
    "response#2": "GIKVVDLLAPYQRGGKIGLFGGAGVGKTVLIMELINNVAKAHGGFSVFAGVGERTREGNDLYREMIESGVIKLGEKQSESKCALVYGQMNEPPGARARVGLTGLTVAEYFRDAEGQDVLLFIDNIFRFTQANSEVSALLGRIPSAVGYQPTLASDLGALQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRQISELGIYPAVDPLDSTSRMLSPHMKQVAGQIKLFLAQYREVASFAQFGSDLDAGTRATLDRGLRLTELLKQPQYSPLAVEEQVPLIYCGVKGYLDKIPVDRVVEFEHKFIPYLRSSGAEIMEAIRKEGVLSKTTEDSLKAVIKEFLSSFDMILNGKYDDLPEQAFYLVGDINEAIAKAEKIKKDA",
    "response#3": "RGGKIGLFGGAGVGKTVLIMELINNVAKAHGGFSVFAGVGERTREGNDLYREMIESGVIKLGEKQSESKCALVYGQMNEPPGARARVGLTGLTVAEYFRDAEGQDVLLFIDNIFRFTQANSEVSALLGRIPSAVGYQPTLASDLGALQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRQISELGIYPAVDPLDSTSRMLSPHILGEEHYNTARGVQKVLQNYKNLQDIIAILGMDELSEDDKLTVARARKIQRFLSQPSFNFPDQVKAMKQVAGQIKLFLAQYREVASFAQFGSDLDAGTRATLDRGLRLTELLKQPQYSPLAVEEQVPLIYCGVKGYLDKIPVDRVVEFEHKFIPYLRSSGAEIMEAIRKEGVLSKTTEDSLKAVIKEAMETYKSSDEE"
  },
  {
    "reference": "MQLVQLLPPGVSLLALVWLAVDAAFLTALLLYLQRGCDSGRSLLCSVFQDLIRYGKTKSGLRRPSWLQWFDIPKRCFWHFYFVSLVWNGFLLWILLHLLLQSVPVPEWLQAVLQFLCAGSEPQVLGGELSVVLAFSLLWLHSLRRLLECLFVSIFSNGVIHFVQYCFGLGYYILIGFTILGYCPLDRRTAVSLDDLLMQGNWYHILGLTLYVWASLHQYTCHCILADLRKSASGAIINLKHAVPTGDWFEKVSCPHYFAELLIYLSIAVVFGLLNTIWWLVVLYVLLSQALAAVLCHEFYHEKFDSYPIHRKAFIPLIF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that contributes to dolichol metabolic process, dolichol-linked oligosaccharide biosynthetic process by functioning in endoplasmic reticulum with cholestenone 5-alpha-reductase activity, 3-oxo-5-alpha-steroid 4-dehydrogenase activity, oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor, polyprenol reductase activity.2. For general function, the protein need meet that Also able to convert testosterone (T) into 5- alpha-dihydrotestosterone (DHT).; Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism; Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol; Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N- glycosylation3. The Cytoplasmic, Lumenal topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.4. The protein should contain a Helical membrane-spanning region.5. The protein design should take into account the physiological conditions of the Protein modification, protein glycosylation pathway. The designed protein sequence is ",
    "response#1": "DVQLTLCWYHILGVSMFIWASIHQHRCLTILADLRKSKSGKVMNLEHSIPFGDWFDMVSCPHYFAELLIYVSMAIMFGFNNLTWWLVVTYVFFNQAVSASLCHEYYLKNFKHYPKCRKAYIPFIN",
    "response#2": "CWYHVVGIVVYIWASLHQHRCLVILSNLRKSKSGKVVSLSHSIPFGDWFERVSCPHYFAELLIYVSMAITLGLDNVTWWLVVTYVLFNQALAAVLCHEFYQTKFTSYPKHRKHK",
    "response#3": "MSWYHVAGAVLFSWASLLQHRSLVLLARLRTGGSGTVETLAHRVPSGGWFELVSCPHYLAELLIYVSLGIVSGSHALTWWLVVLYVLFNQALAAQLCHEFYTSKFQSYPQHRKAFLPYLS"
  },
  {
    "reference": "MPIRALCTICSDFFDHSRDVAAIHCGHTFHLQCLIQWFETAPSRTCPQCRIQVGKRTIINKLFFDLAQEEENVLDAEFLKNELDNVRAQLSQKDKEKRDSQVIIDTLRDTLEERNATVVSLQQALGKAEMLCSTLKKQMKYLEQQQDETKQAQEEARRLRSKMKTMEQIELLLQSQRPEVEEMIRDMGVGQSAVEQLAVYCVSLKKEYENLKEARKASGEVADKLRKDLFSSRSKLQTVYSELDQAKLELKSAQKDLQSADKEIMSLKKKLTMLQETLNLPPVASETVDRLVLESPAPVEVNLKLRRPSFRDDIDLNATFDVDTPPARPSSSQHGYYEKLCLEKSHSPIQDVPKKICKGPRKESQLSLGGQSCAGEPDEELVGAFPIFVRNAILGQKQPKRPRSESSCSKDVVRTGFDGLGGRTKFIQPTDTVMIRPLPVKPKTKVKQRVRVKTVPSLFQAKLDTFLWS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The target protein must exhibit helix as its primary conformation. The designed protein sequence is ",
    "response#1": "WEIIETIGKGTYGKVYKVANKRDGSLAAVKVLDPVSDMDEEIEAEYNILQFLPSHPNVVKFYGMFYKADRCVGGQLWLVLELCNGGSVTELVKGLLRCGKRLDEAVISYILYGALLGLQHLHCHRIIHRDVKGNNILLTTEGGVKLVDFGVSAQLTSTRLRRNTSVGTPFWMAPEVIACEQQYDSSYDARCDVWSLGITAIELGDGDPPLFEMHPVKMLFKIPRNPPPTLLHPDSWCEEFNHFISQCLIKDFEKRPSVTHLLDHPFL",
    "response#2": "WVRLALCLTGLVLSLYALHVKAARARDRDYRALCDVGTAISCSRVFSSRWGRGFGLVEHVLGQDSILNQSNSIFGCIFYTLQLLLGCLRTRWASVLMLLSSLVSLAGSVYLAWILFFVLYDFCIVCITTYAINVSLMWLY",
    "response#3": "EIIETIGKGTYGKVYKVANKRDGSLAAVKVLDPVSDMDEEIEAEYNILQFLPSHPNVVKFYGMFYKADRCVGGQLWLVLELCNGGSVTELVKGLLRCGKRLDEAVISYILYGALLGLQHLHCHRIIHRDVKGNNILLTTEGGVKLVDFGVSAQLTSTRLRRNTSVGTPFWMAPEVIACEQQYDSSYDARCDVWSLGITAIELGDGDPPLFEMHPVKMLFKIPRNPPPTLLHPDSWCEEFNHFISQCLIKDFEKRPSVTHLLDHPFF"
  },
  {
    "reference": "LECHNQQSSQPPTTKTCSGETNCYKKWWSDHRGTIIERGCGCPKVKPGVNLNCCRTDRCNN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The engineered protein is expected to adopt a strand-strand-strand-strand-strand-based conformation.2. The protein can be targeted to extracellular region and perform toxin activity, acetylcholine receptor inhibitor activity, ion channel regulator activity.3. The protein must exhibit the following characteristics: Binds to muscle nicotinic acetylcholine receptor (nAChR) and inhibit acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission.. The designed protein sequence is ",
    "response#1": "EEKLMDDLLNKTRYNNLIRPATSSSQLISIKLQLSLAQLISVNEREQIMTTNVWLKQEWTDYRLTWNSSRYEGVNILRIPAKRIWLPDIVLYNNADGTYEVSVYTNLIVRSNGSVLWLPPAIYKSACKIEVKYFPFDQQNCTLKFRSWTYDHTEIDMVLMTPTASMDDFTPSGEWDIVALPGRRTVNPQDPSYVDVTYDFIIKRKK",
    "response#2": "EERLINDLLIVNKYNKHVRPVKHNNEVVNIALSLTLSNLISLKETDETLTSNVWMDHAWYDHRLTWNASEYSDISILRLPPELVWIPDIVLQNNNDGQYHVAYFCNVLVRPNGYVTWLPPAIFRSSCPINVLYFPFDWQNCSLKFTALNYDANEITMDLMTDTIDGKDYPIEWIIIDPEAFTENGEWEIIHKPAKKNIYPDKFPNGTNYQDVTFYLIIRRKR",
    "response#3": "EEKLMDDLLNKTRYNNLIRPATSSSQLISIKLQLSLAQLISVNEREQIMTTNVWLKQEWTDYRLTWNSSRYEGVNILRIPAKRIWLPDIVLYNNADGTYEVSVYTNLIVRSNGSVLWLPPAIYKSACKIEVKYFPFDQQNCTLKFRSWTYDHTEIDMVLMTPTASMDDFTPSGEWDIVALPGRRTVNPQDPSYVDVTYDFIIKRKK"
  },
  {
    "reference": "MYRQLRLFAGTSNPKLASDISQQLGVDLGAIKISRFQSGEIYVHYEESIRNCDVFLVQSFSHPINEMFVELLVMIDAAKRASARTINIIVPYYGYARQERKAAPREPISAKMVADVLTTAGASRVITIDLHAPAIQGFFNIPVDHMTALDLITDYLKSKRIVCPVIVSPDAGRAKTAEKLASRLDSPFAIMIKQRPAHNESVITHVIGDVTGRTPIIIEDLIDTGSTIVQVVESLKERGANNAIVCATHGLFSGEALDRLDHAHIEEIVVTDSIVLPEHGSKTKLNVLSVAPMLAQATQIIMEGGSIATLFKDAGV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be able to regulate 5-phosphoribose 1-diphosphate biosynthetic process. The designed protein sequence is ",
    "response#1": "AEPQIIKYIRKYIPGEKVIVSPDAGGVKRAKSISDKLDADLAIIHKERKKANEVSGMILVGDVKDKVALIVDDMADTCGTLVSACEMLISKGATKVYALVTHGVLSGDAIQKLNESSLTELVITNG",
    "response#2": "AEPQIIKYIRKYIPGEKVIVSPDAGGVKRAKSISDKLDADLAIIHKERKKANEVSGMILVGDVKDKVALIVDDMADTCGTLVSACEMLISKGATKVYALVTHGVLSGDAIQKLNESSLTELVITNR",
    "response#3": "PILLDDMQNRELDNPVVVSPDIGGVVRARAVAKLLDDADLAIIDKRRPKANVAQVMHIIGEVEGRDCIIVDDMIDTGGTLAKAAEALKEHGAKRVIAYATHPVLSGNAAQNLRESVIDEVVVTDAR"
  },
  {
    "reference": "MGKYQPSFQTKKGWTEVICGPMFSGKTEKLLHKIKRWKIAKISVVIFKPIIDTRQTNIVKSRNGEYDQAITINSPFEIYDHLVDKNYQIVAIDEAQFFSNEIIEVVTTLNEIGTNVIISGLDTDFRAEPFGCIPQLLAIADVVNKLDAICNVCGSLAQRTQRLVNKNTNDNLVLIGDAEAYEARCKLHHSFLTKKHVTVKTKNFKEQVQGKTQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.2. The ATP binding site should be stable and able to withstand changes in environmental conditions.3. The designed protein must be optimized for thymidine kinase activity. The designed protein sequence is ",
    "response#1": "MRSLL",
    "response#2": "MAAIVGGNDRYVSCCRKHG",
    "response#3": "MTAVPGQP"
  },
  {
    "reference": "MSVERTTARGGMETSYGFRQVGAGEKQPLVNEVFHKVANRYDLMNDLMSAGMHRIWKDAMIAWLNPPKRPGWKGLDVAGGTGDIAFRIIEASRRNAHETVLDINGSMLQVGRERAEKKGLSPYLDFVEANAEELPFADSTFDAYTIAFGIRNVPRIDVALSEAFRVLKPGGRFLCLEFSDVDMPLLDRFYEAWSFNAIPKIGEMVTGDGEPYAYLVESIRKFPDQKNFAEMISSAGFERVSFRNYSGGIAALHSGWKL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for activity in Quinol/quinone metabolism, menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2, menaquinone biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The protein must exhibit the following characteristics: Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2)..3. The protein should have a fast turnover rate for a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine.4. The S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MSDIAKKKNVPEKTSFGLSDEEKAKKVAGVFHKVASRYDLMNDLMSLGIHRLWKRFTVETSAVRTGQRVLDLAGGTGDLTAKFSRMVGKEGQVVLADINDSMLRVGRDKLIDKGIVGNIEYVQANAECLPFPSDYFDCVCIAFGLRNVTDKDKALRSMYRVLKPGGVFAILELSKPTSETLSKAYDAYSFHLLPKIGKLITNDSDSYRYLAESIRMHPDQETLKGMMEAAGFEKVEYFNMTGGVVALHRGYKF",
    "response#2": "MALAPLRDLLPQYRPNDPCADAARELDRVDQSLIESLADRMYDFLNHFLSLRRDIAAHRQTYGVPDVAVLAGGAQRAVDLACAALNVLYPGQSIDPFLRAMPKYESKPPAMPYGKPLFDWSPA",
    "response#3": "MSAQPFGYRDVPVAEKAGRVRGVFDSVASNYDFLNHFLSLRRDIAWRRFAVERSGVRAGDKVLDIAGGTGDLAAAFSRIVGSTGEVVLADINAAMLGVGRDRLLNRGVHGNVEFVQADAESLPFPDTHFDVVTIAFGIRNFTDKSRALSEMARVLKPGGVFAILELSKPTNPLFAKIYDAYSFKALPFMGKAIANDADSYRYLAESIRMHPDQETLKGMMEDAGFARTRYHNMTGGIVALHVGIKP"
  },
  {
    "reference": "MIKGKKIILGISGGIAAYKMTNVASMLYKLGADVHVIMTKNACNFITPKTFEVLTKNKVYIDTFDEEPDDAVNVPHISLGQSADCFLIAPATANIIAKLAHGIADDMLTTTVLPARCPMLICPSMNGYMLDNSVTQENIEILRKRGYKVIDSEFGNLACGYEGKGKLPKEEVLVEHILNAVEFDHDMEGKKVMVSAGPTEEPLDPVRIITNHSSGKMGYALARVAAKRGAKVTLVSGPVNLETPLNVERVNITTANDMYEEITKRAGDQDIIIMAAAVADYTPETVAENKIKKKEGGMSIPLKRTHDILKTLGENKKDGQFICGFSMETENLLENSSAKLEKKNADMICANSLTVPGAGYKVDTNIITLIKKVEGSDKPVMRELPLMSKDEAAFEILSEIIGR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.2. The designed protein should be optimized for phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity, FMN binding activity in order to improve coenzyme A biosynthetic process.3. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A4. A protein that can modulate the overall flux of metabolites through the CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MNIILGICGSIASYKAATLVRLLVKSGAAVKVVMTPDATHFITPLTLSTLSKNPVLVEYANAQTGEWNNHVELALWADYLVIAPASANTLAKMANGLCDNLLTAVYLSAKCPVYVAPAMDLDMWKHASTQANVGRLQSYGNILIRPGYGELASGLTGEGRMAEPEEIAEAICQAINPVLFKGLSGRKILVSAGPTWEPFDPVRFITNPSSGKMGYALAAEASRRGAETHLVSGPSVLCPPSGVRFHGVVTAEEMNRVVSDLAPAMDAVIMAAAVSDFRPEKAAPQKTKKDSNDLTVRLVRTPDILDGLGSRKPAGQVLVGFAAETENLLENAAEKLVRKNLDIIVANDLTQPDSGFRCDTNQVKFITKDNVEDWPDMAKDQVAEKLAEKIAEYL",
    "response#2": "MHIVLGVSGGIAAYKAVLLLRLLREAGHDVRVVPTRAALEFVGRPTWEALSGQPVTTDVFEDVDQVAHVAIGRRADLVVVAPATADLLARAAAGRADDLLTATLLVTRAPVLLAPAMHTEMWEHPATVANVATLRARGVHVLDPASGRLTGADTGPGRLPEPDEIARAALAAALHALAA",
    "response#3": "MHIVLGVSGGIAAYKAVLLLRLLREAGHDVRVVPTRAALEFVGRPTWEALSGQPVTTDVFEDVDQVAHVAIGRRADLVVVAPATADLLARAAAGRADDLLTATLLVTRAPVLLAPAMHTEMWEHPATVANVATLRARGVHVLDPASGRLTGADTGPGRLPEPDEIARAALAAAARELLGPRPLAGRHVVVSAGGTREPLDPVRYLGNRSSGRQGVALAAAALERGARVTLVGANLSVAAPPGADLVAVGTAAELRDAVRAAGKDADVVVMAAAVADYRPVSPADAKIKKQPGGPVPTITLVETVDVLAEIAHERLRAGQVVVGFAAETGDDEGSVLEHGRAKARRKGADLLVVNAVGDGLGFGTADNDVTILGADGTETPVPYGPKEALADTVWDLVAARLG"
  },
  {
    "reference": "MTDPDPSPRNDSSQDPSRRPSGAPRRDRNPAGNSLWYVLIFCVLGAVAISVYRGQTSGKELTFSEFEAKVRSGEFNKDNVHELTFGQQYLTFQDQPKGKSLGIDSEDAAEPSGETAADDPQAPEGEEQAAAEEPAEEAPAQSSDSVQRYRVAIGFMPEASLAELDKLLRERGIQVKSTTIGTQWESVIYFVFMILLIVVVITFFRRMGGAGSAMSFGRSRGRLVAEDDIKVTFDDVAGIEEAVEELREVVEFLRTPSKYQALGGRIPRGVLLVGPPGTGKTLLAKAVAGEAGVPFFSLSGSDFVEMFVGVGAARVRDMFAQAVQRSPSIIFIDELDALGKVRGSGMPGGHDEREQTLNALLVEMDGFSSDQSVIVMGATNRPETLDPALMRPGRFDRHVLVDRPDYKGREAILKVHSAKIKLDDNVDLGRLAKLTPGFVGADLANLVNEAALLAARKNKSAASMAEFEEGVERVIAGLEKSTRIIHEDEKQRVAYHECGHALVACSLPHTDPVHKISIIPRGMGALGYTLQRPEDDRHLVTQTELQNRICVLLGGISAEELVYNETSTGAQNDLERATDIARRMVTEFGMSPKLGRVNYQESRRSMFLGNTMTMAPEYTHSADTVREIDLEVKRIVDECMQTADDVLKTQRDALEAMTRELIEIEVMDADRLAKILDAHRTGPQIKPGTFVSRTGGEAPESQQSDTDESSRKADGGA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be able to bind Zn(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "RNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPSSPATKNPCNIHYSPNDEDKGMVLPGTKCADGKACSNGRCVDC",
    "response#2": "IPKGVLLYGPPGTGKTLLARAVAGEAGVPFFALSGSSFVELFVGMGASRVRELFAQARKNAPCIVFIDEIDAVGRQRGSAAVVGGHDEREQTLNQLLTEMDGFGAYEGVIVMAATNRPDVLDKALLRPGRFDRQIPVGPPE",
    "response#3": "LPTTTIGSFPQVAGVRSARRSFKQGRISEEQYRAEMQRYIREAIAQQEALGLDVLVHGEPERNDMVEYFGEQLAGFCFTENGWVQGYGSRCVKPPIIYGDVERLKPMTMEWTTYAQQQTNRPLKGMLTGPVTMLCWSFVRDDQPRSATCKQIALAIRDEVQDLEQAGIKIIQVDEAALREGLPLRKSAWREYLGWAVDAFRLTASGVADSTQIHTHMCYSEFNHIAEWIAKMDADVISIEASRSNMELLGVLESFTYPNDIGPGVYDIHSPRVPSVAEMVELLRKASEVIPLERLWVNPDCGLKTRAWPETLASLRNMVAAAR"
  },
  {
    "reference": "MQVTDVRLRRVNTEGRMKAIASITIDHEFVVHDIRVIDGNNGLFVAMPSKRTPDGEFRDIAHPISSQTREKIQTAVLAEYERAGELDQVTYEEAGAS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Interferes with or is a negative regulator of the pathway leading to asymmetric septation.; Essential for sporulation The designed protein sequence is ",
    "response#1": "CLQSLLDGEKVYGTSDKDFYVPSKNVEYFREYFLCIRDLSQYYKPIAVREWEKTLDSGEILWPSLAITAQGRCPFI",
    "response#2": "NEKPLGDQRAHLPDSFLELEQEIAIREEGLSKLFQATTIWIDSILKKVDGEDKEKCLACENLGKVMINHSKELPQDSSYGITLSQLGKANLKIGEHQTSLAYKARVCYLDFLKRYLVQAKDFHSARKKLESRRQAYESLLQKSFKEKKEDSRLEEDIRLALYKFEESTEQVKNRMIALKDVEADQYQQLTELIVYELNFFKESTGILNTIFNSQNSLTPQKKR",
    "response#3": "VSRFFSWTNEKPLGDQRAHLPDSFLELEQEIAIREEGLSKLFQATTIWIDSILKKVDGEDKEKCLACENLGKVMINHSKELPQDSSYGITLSQLGKANLKIGEHQTSLAYKARVCYLDFLKRYLVQAKDFHSARKKLESRRQAYESLLQKSFKEKKEDSRLEEDIRLALYKFEESTEQVKNRMIALKDVEADQYQQLTELIVYELNFFKESTGILV"
  },
  {
    "reference": "MAPVSNQVGGHVAVLAFPFSTHAAPLLNIVCRLAAAAPSTLFSFFNTKQSNSSILAGNTSVLRYSNVSVCEVADGVPEGYVFVGKPQEDIELFMKAAPDNFRRCLEASVAESGREVSCLVTDAFFWFGVHMADDMGGVPWVPFWTAGPASLSAHVHTDLIRSTTSGGCHDEKETITVIAGMSKVRPQDLPEGIIFGNLESLFSRMLHQMGQMPPLATAVFINSFEELDPVITNDLKSKFKRFLNVGPLDLLEPPASAATTTPQTAAEAVAGDGCLSWLDEQKVASVVYVSFGSVTRPSPEELMALAEALEASRVPFLWSLRDNLKNRQLDEFLSKGKLNGMVVPWAPQPQVLAHGSVGAFVTHCGWNSVLESVAGGVPLICRPFFGDQKLNARMVEDVWKIGLRLEGGVFTKNGMLKSLDMLLSQDKGTKMKNKINTLKQFAKQAVEPKGSSARNFESLLEMTTTN",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that In the presence of other necessary color factors, this glycosylation reaction allows the accumulation of anthocyanin pigments (Probable); Uses UDP-Glc as a sugar donor, but not UDP-Gal or UDP-GlcUA2. A protein with myricetin 3-O-glucosyltransferase activity, flavonol 3-O-glucosyltransferase activity is needed to enhance anthocyanin-containing compound biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "WCSQTAVLAHCAVGCFVTHCGWNSTLESLESGVPVVAFPQFADR",
    "response#2": "WAPQVEILEHAALGVFVTHCGWNSILESIVGGVPMICRPFFGDC",
    "response#3": "VGFWS"
  },
  {
    "reference": "MDSWNMKGSKMVDVNEITKYLPGFNCGACGYKRCDLFAEALLNKDVKLEDCPFLLRERFKENYEKLKEILKIKGKIKKEEKYIGVIDGYEADFLLKPLPNECSCRETLLIMDKKELKVGDYIRYRPLGCPIPHFAKIIDEYHGFYIIHVVGPSHRITGEKIEYKDVGIAIVVAFEGIVEGKVPEVGKTVKFIPKHCMMQKVHSGVVVQVEGKRVYIEGIDLKVF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The presence of [4Fe-4S] cluster is necessary for the protein to function in the desired environment.2. The protein should be able to bind [4Fe-4S] cluster ligand in a variety of conditions3. The protein must be able to metal ion binding. The designed protein sequence is ",
    "response#1": "SGGASLQVAKNILESSFKLEAGTSSMGAVVSLVQTPGLHSPAYLAEVARVLVSGGDLIVQEPLLAEAQEQKCSSAQTKAQLERNLLLAGFVNLEVVDSVVGVEIAKACTTSSVALNVVAVKSSKPSWDTGC",
    "response#2": "SGGASLQVAKNILESSFKLEAGTSSMGAVVSLVQTPGLHSPAYLAEVARVLVSGGDLIVQEPLLAEAQEQKCSSAQTKAQLERNLLLAGFVNLEVVDSVVGVEIAKACTTSSVALNVVAVKSSKPSWDTGC",
    "response#3": "SGGASLQVAKNILESSFKLEAGTSSMGAVVSLVQTPGLHSPAYLAEVARVLVSGGDLIVQEPLLAEAQEQKCSSAQTKAQLERNLLLAGFVNLEVVDSVVGVEIAKACTTSSVALNVVAVKSSKPSWDTGC"
  },
  {
    "reference": "MKQLINNDWWPVLKPQFETANYQQLHNFLVDEYGHQQVYPEMHHIFEAFNWTPFSKVKVVILGQDPYHGPGQAHGCSFSVLPGVAVPPSLQNIYKELQADLGCPPVKHGYLRSWAEQGVLLLNSVLTVRAGQAYSHQGHGWEQLTDAAIVALSKRPTPVVFILWGRAARDKKRLIDLKRNFVVESAHPSPLSAYRGFFGSRPFSKTNQFLEMTGQAPINWQLPSTVDHL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.2. A protein that can perform uracil DNA N-glycosylase activity in cytoplasm to support base-excision repair.3. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for Hydrolyzes single-stranded DNA or mismatched double-stranded   DNA and polynucleotides, releasing free uracil.. The designed protein sequence is ",
    "response#1": "PKEVLFLGMNPGPFGMAQTGVPFGEVNVVRDWLGVGGPVLTPPQEHPKRPVLGLECPQSEVSGARFWGFFRTLCGQPQVFFRHCFVHNLCPLLFLAPSGRNLTPAELPAKQREQLLSICDAALCRQVQLLGVRLVVGVGRLAEQRARRALAGLTPEVQVEGLLHPSPRSAQANKGWEAAAL",
    "response#2": "TPFHDVKVVILGQDPYHGANQAMGLSFSVPSGVRVPPSLVNIYKEIYADLGIKEPNSGDLTKWAKQGVLLLNSTLSVSAGAANSHASFGWQGFTDAVIRKISENLQNVVFMLWGNPAKAKAPLIDASKHLILEAAHPSPLARGAFFGCRHFSKANIYL",
    "response#3": "FGDVKVVILGQDPYHGPNQAHGLCFSVLPGVKTPPSLVNIYKELAQDIPGFQIPPHGYLQSWAQQGVLLLNTVLTVEQGMAHSHANTGWETFTDRVIDALNQHRNGLIFLLWGSHAQKKGQMIDRQRHHVLMAPHPSPLSAHRGFLGCRHFSKTNQLLL"
  },
  {
    "reference": "MSNQQRGSNETTHFGYEQVQVDDKEKRVADVFHSVAAKYDLMNDLMSMGVHRLWKAIAIEYSGVRTGQRVLDIAGGTGDLTMRFSKLVGPTGQVVLADINDSMLKVGRDKLQDRGVSGNVRFVQANAEMLPFPDNHFDCITIAFGLRNVTDKQKALESMARVLKPGGRLLVLEFSKTNNPLLTKMYDTYSFSLLPMMGKLIVNDSESYKYLAESIRMHPDQETLKGMMEQAGLVRCSFHNMTGGIVALHKGIKP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should take into account the physiological conditions of the menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2 pathway. The designed protein sequence is ",
    "response#1": "GQDKW",
    "response#2": "EVLL",
    "response#3": "MLDVF"
  },
  {
    "reference": "MKIGVLTGGGDSPAINAAIRAVVYQAKAYDFELTGIRNGWAGLVEANFSPLTVDTVEGTLTKSGTVLGTSRTNPFKTNTVDKVVENFNKEKLDALIAIGGDDTLGVLSKLHHNDKPINGVGIPQTIDNDIIGTDYAIGFDSAVTIATDAIDKLRSTAESHHRVMVLEVMGRDAGHISLRSGVAGGAHVILVPEEDFDLTEICKFLKKRKEAGKIYSIIVVAEGAKPKDGMQMAQREVKDQFGHVMLGGISSYLVDEIEKCTKIESRLTILGHLQRGGVPTSFDRFMATRFGVRAVKMVEKGAFDQMVALKGKDLFAVGLKEGLAKTKPINMDFYQQIKMLF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for beta-D-fructose 6-phosphate + diphosphate = beta-D-fructose   1,6-bisphosphate + H(+) + phosphate.2. The designed protein should contain one or more Phosphofructokinase domains that are essential for its function.3. The designed protein should be involved in fructose 6-phosphate metabolic process by localizing to cytoplasm and exhibiting metal ion binding, diphosphate-fructose-6-phosphate 1-phosphotransferase activity, 6-phosphofructokinase activity.4. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.5. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis; Consistently, PPi-PFK can replace the enzymes of both the forward (ATP- PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.; Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis.6. The protein should have a high affinity for substrate, Mg(2+). The designed protein sequence is ",
    "response#1": "NVGVILSGGQAPGGHNVIAGIFDGIKRLHKESKLYGFILGPAGLIDHQYKELTSEIIDEYRNTGGFDIIGSGRTKLETKEQFDKGLIILKKLDIKALVIIGGDDSNTNACVLAEYYKSINAGVQVLGCPKTIDGDLKNEQIETSFGFDTACKVYSELIGNIQRDCNSSRKYWHFIKVMGRSASHIALECALQTHPNVCIISEEVEEKDLSLDDIIEYIAGVIAKRAENGLNFGTVIIPEGLIEFITGRASNVTIVDIRRIGVLTGGGDSSGINACIRAIVRRAIKEGLEVIGFKDGWRGPIENDAIPLTLDSVSGILPQGGTILGTSRTNPFRKVQKDGETVWEPTEKVERILQNLKSRGIDALIPIGGDDTLGVAWRLYENYGVKVVGIPQTIDNDVAGTDYCLGFHSALAVVTEALDRLHTTAQSHHRVFVVEVMGRDAGWIALLGGIAGGGDVILVPEEPFSVDEVVKGIEARLSRGRTFSIIVVAEGARPVELGGKQVAQEGRVDAFGHVQLGGIGYWLADRLRERGMECRVVVLGHLQRGGIATPFDRLLATRFGVAAVEAVHEGFGSMVVGLRGERIVKVPIAEATTRLKKVPLELYEEAEVFFG",
    "response#2": "VGVILSGGPAPGGHNVIAGLFDALIKANPQSRLLGFKSGPSGLIKGQYVELSSELIDQYRNTGGFDMIGTGRTKIETSEQFEQSLENAIKLGLDALVIIGGDDSNTNAALLSEYFFQKGAPITVVGVPKTIDGDLKNEWIETSFGFDTATRVYSELIGNICKDASSGRKYWHFVKLMGRSASHITLECAFQTRPNVALISEEVDAKKMSLSQVVEIIANTIAARSAKGENFGVVVVPEGLIEFIPEIKTLIAQINDIIEFIPEIKKSLSENFKFKKENEIINIAKGIKNLESDKIKIRVTVLGHVQRGGTPTAYDKIGVVLSGGQAPGGHNVISGIFDYLQDRTKGSTLYGFGGGPAGIMKCKYVELTTDFIYPYRNQGGFDMICSGRDKIETPEQFKQTEETALKLDLDGLVVIGGDDSNTNACLLAENFRGKNMKTRVIGCPKTIDGDLKSKEVPTSFGFDTACKIYAEMIGNVMTDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVAAKKQTLKNVTDYITDIICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDVVDEAGQWKKKLRSQSQQLFEFLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRRQEGSYNKQFKGQSHFFGYEGRCGLPTNFDANYCYALGYAGGVISKEKRIGVLTGGGDSSGINACIRAIVRRAIKEGLEVIGFKDGWRGPIENDAIPLTLDSVSGILPQGGTILGTSRTNPFRKVQKDGETVWEPTEKVERILQNLKSRGIDALIPIGGDDTLGVAWRLYENYGVKVVGIPQTIDNDVAGTDYCLGFHSALAVVTEALDRLHTTAQSHHRVFVVEVMGRDAGWIALLGGIAGGGDVILVPEEPFSVDEVVKGIEARLSRGRTFSIIVVAEGARPVELGGKQVAQEGRVDAFGHVQLGGIGYWLADRLRERGMECRVVVLGHLQRGGIATPFDRLLATRFGVAAVE",
    "response#3": "MQYRVGVLTGGGDAPGLNAAIRGVARAAWREGYEVVGIRNGWQGLLRNDVFPLTPDAISGILPRGGTILGTSRTNPFKTSEGPRQVLDTVRTRGIDVVVAIGGDDTLGVAARLAKMGMRVVGIPKTMDNDIPGSDYTIGFDTAVTAVMEACDKLHPTAEAHQRVMVVEVMGRDAGWVAAVGGLAGGADVVLVPEVPFSVDEVCQRIKDRHERGKLFSVLVVAEGCKPKDLGTQVAQDAAVDAFGHVRLGGIGSVLAGEIEARTGYETRVTVLGHLQRGGSPTWFDRVLATRFGVAAVELACQNQFGRMVALQGNSIRVAFVXSGGQAAGXHNVASGLFDALXKMNDQSQLFGYLEGPKGVIENKYREILETELAAYRNQGGFDLIGSGRTKIETEEQFIAAAKSVSDLRLDGLVIIGGDDSNTNAALLAEYFLDHNIQTKVIGVPKTIDGDLXNEFIEIPFGFDSATKTYAEIIGNLLVDALSAKXYTFFIKLMGRTASHIALECAXKTHANLTLIGEEIESQKKTLREITTEIVDLLVERSKQGKNYGLILIPEGLIECVVPEANLRGEFERVGVLTGGGDAPGLNAAIRGVARAAWREGYEVVGIRNGWQGLLRNDVFPLTPDAISGILPRGGTILGTSRTNPFKTSEGPRQVLDTVRTRGIDVVVAIGGDDTLGVAARLAKMGMRVVGIPKTMDNDIPGSDYTIGFDTAVTAVMEACDKLHPTAEAHQRVMVVEVMGRDAGWVAAVGGLAGGADVVLVPEVPFSVDEVCQRIKDRHERGKLFSVLVVAEGCKPKDLGTQVAQDAAVDAFGHVRLGGIGSVLAGEIEARTGYETRVTVLGHLQRGGSPTWFDRVLATRFGVAAVEL"
  },
  {
    "reference": "HPITESAEMPYPGPASLEERGVGSLDDLSLSEQNYPPQRGAGLRYATLEVLLEKQSLLNPFSRVFGIRKQFAGTTECFWKYCV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be expressed specifically in the extracellular region The designed protein sequence is ",
    "response#1": "LMQFETLIMKIAGRSGVWWYGSYGCYCGAGGQGRPQDPSDRCCFVHDCCYGKVTGCNTKDEFYTYSEENGAITCGGENPCLKEVCECDLAAAICFRDNLDTYNSKKYWMFPAKNC",
    "response#2": "GKWYGIGLASNSNWFQSKKQQLKMCTTVITPTADGNLDVVATFPKLDRCEKKSMTYIKTEQPGRFLSKSPRYGSDHVIRVVESNYDEYTLMHTIKTKGNEVNTIVSLFGRRKTLSPELLDKFQQFAKEQGLTDDNILILPQTI",
    "response#3": "GKWYGIGLASNSNWFQSKKQQLKMCTTVITPTADGNLDVVATFPKLDRCEKKSMTYIKTEQPGRFLSKSPRYGSDHVIRVVESNYDEYTLMHTIKTKGNEVNTIVSLFGRRKTLSPELLDKFQQFAKEQGLTDDNILILPQTV"
  },
  {
    "reference": "MKGKTVSDTSDARPPSPETKTGSRSESENPAMPSPTPKAHAPLKNQDWWPNQVDVSVLHAQTEKANPLGADFDYREQVKTLDAEALKRDLIELMTTSQEWWPADYGHYGGAFIRMSWHAAGTYRIHDGRGGGGQGAQRFAPLNSWPDNSGTDKSRRLLWPIKQKYGNKISWADLIIVAGNVALESMGFKTAGFAFGREDIWEPEETLWGFEDTWLGTDKRYSGERELAEPYGATTMGLIYVNPEGPEGEPDPLKAAYDIRETFGRMAMNDEETAALIVGGHTFGKTHGAGDPDLVGPEPEGAPIEQQGLGWKSGYGSGKGADAIIGGPEVVWTPTPTQWDNTFLEYLYGHEWELEQSPAGAWQYVAKDVEPSIPEPFDAAKKRMATMLVTDISMRESPIYADITRRWLDHPEELADAFARAWFKLLHRDMGPASRYIGPWVPKESYVWQDPVPAVDHPLVDEQDIATLKNKVLASQLSVSQLIKTAWASAASFRGTDKRGGANGARIRLEPQRNWEVNEPAELAKVLPVLEQIQQDFNGSASGGKKISLADLIVLAGSAAVEKAAKDAGFDIDVHFAPGRTDATQEQTDVESFAIIEPRADGFRNYLRPNEKTQVERLLVDRAYMLNLTAPELTVLIGGLRSLGANHGGAGHGVFTDRPGVLTNDFFVNLLDINTQWKGSTAENVYEGVDRASGQTKWTATANDLVFGSHSQLRALAEVYAQDDNQGKFVEDFVAAWVKVMNNDRFDLD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must possess catalase activity to support hydrogen peroxide catabolic process under certain conditions.2. The presence of Plant heme peroxidase family profile domains is critical for the interaction of the designed protein with its intended targets.3. The protein should be able to bind heme b ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "LSWGDLMVLAGNVALEDMGFRTVGFAGGRIDAWQPDVVFWGPETKMMGTERSNPDGSLKRSLAATTMGLIYVNPEGPNGNHDPIGAAADIRRAFGQMAMDDEETLALIAGGHTFGKAHGAKKPDQCLDAEPTAAGMEEQGLGWRNKCGKGNAEDTLTSGLEGAWSAAPTQFTSQYIDNLLNFEWVKTKSPAGATQWIPTDPAAAELVPDAHVEGKRHAPIMFTTDIALKEDPGFRKVLESWQKNPELFSKAFARAWFKLTHRDLGPQGRYD",
    "response#2": "LDKARRLLWPIKQKYGNKISWADLMILTGNVALESMGFKTLGFGGGRADTWEPDQDVYWGRETKWLGGDVRYSQGSPGVEGRGVLVKDDDSKAPESRDLENPLAAVQMGLIYVNPEGPDGKPDPVASARDIRDTFGRMGMDDEETVALIAGGHTFGKTHGAAPADNVGVEPEAEDLGTQGFGWHNKHGAGKGADAITSGLEVTWTRTPTQWSNDYFEFLFGHEWELTKSPAGAHQWVAKNAEPSIPHAFDAGKKLLPTMLTSDLALRFDPAYEAISRRFLANPDQFADAFARAWFKLTHRDMGPRARYLGKDVPAN",
    "response#3": "LSWGDLMVLAGNVALEDMGFRTVGFAGGRIDAWQPDVVFWGPETKMMGTERSNPDGSLKRSLAATTMGLIYVNPEGPNGNHDPIGAAADIRRAFGQMAMDDEETLALIAGGHTFGKAHGAKKPDQCLDAEPTAAGMEEQGLGWRNKCGKGNAEDTLTSGLEGAWSAAPTQFTSQYIDNLLNFEWVKTKSPAGATQWIPTDPAAAELVPDAHVEGKRHAPIMFTTDIALKEDPGFRKVLESWQKNPELFSKAFARAWFKLTHRDLGPQGRYS"
  },
  {
    "reference": "MFESLKNFVKTNIEDLDGKELEISKEFKEHHNKDSKYIIKNWIFESQQYRKWRITKLDGGDKLQVFNTVAYPNFKSEFPILGADILWFGTSQKLLAIFDYQPLIQEKKYLQKYCSSLDFIKNQYSVFDNHKMKNIYDSKKYFSPWVMICRGNKLNLDRDLNNIFCSFVSNYLTINKLHQNNQFLDLEQIKNNQIDYDKYSAEKDPADKLFKTFFGETWTENFINNFLFTLNHNPLK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in phytochromobilin biosynthetic process, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "MPKSVALLRIRYTLEAALARGFTTVRDCGGAEGFLKAEIRQGSLNGPRLITCGHAISQTGGHGDLRSGALPASAFDSCSCHFGQVGVVADGVPECYKAAREEFRRGADFIKIMGGGGVASPTDKISNKQFCDDEIKALVDVANSYHTYVTAHAYTAEAIQNCIKLGVKGIEHGNLLDERTAELMAELGCYLTPTLVTYKVMGSDQFSAFLGPENSRKNTEVLYKGIDAMKIAQKKKVKICFGSDLLGPLYGYQTQEFRIRGKVQTTQEILLSATVTPAEMNGLGDKLGQIKPGFIADLLMMKSNPLDDITILDEPESNILFVMKEGRK",
    "response#2": "MPKSVALLRIRYTLEAALARGFTTVRDCGGAEGFLKAEIRQGSLNGPRLITCGHAISQTGGHGDLRSGALPASAFDSCSCHFGQVGVVADGVPECYKAAREEFRRGADFIKIMGGGGVASPTDKISNKQFCDDEIKALVDVANSYHTYVTAHAYTAEAIQNCIKLGVKGIEHGNLLDERTAELMAELGCYLTPTLVTYKVMGSDQFSAFLGPENSRKNTEVLYKGIDAMKIAQKKKVKICFGSDLLGPLYGYQTQEFRIRGKVQTTQEILLSATVTPAEMNGLGDKLGQIKPGFIADLLMMKSNPLDDITILDEPESNILFVMKEGRW",
    "response#3": "MPKSVALLRIRYTLEAALARGFTTVRDCGGAEGFLKAEIRQGSLNGPRLITCGHAISQTGGHGDLRSGALPASAFDSCSCHFGQVGVVADGVPECYKAAREEFRRGADFIKIMGGGGVASPTDKISNKQFCDDEIKALVDVANSYHTYVTAHAYTAEAIQNCIKLGVKGIEHGNLLDERTAELMAELGCYLTPTLVTYKVMGSDQFSAFLGPENSRKNTEVLYKGIDAMKIAQKKKVKICFGSDLLGPLYGYQTQEFRIRGKVQTTQEILLSATVTPAEMNGLGDKLGQIKPGFIADLLMMKSNPLDDITILDEPESNILFVMKEGRR"
  },
  {
    "reference": "MVPHYQQASSCESNTMTAMDEYQHHEDATIPIAIIGMSCRFPGNATSPEKLWELCAEGRSAWSKIPKSRFRQEGFYNPNAERVGTSHVVGGHFLEEDPSLFDASFFNLSAEAAKTMDPQFRLQLESVYEAMESAGITLEHIAGSDTSVYAGACFRDYHDSLVRDPDLVPRFLLTGNGAAMSSNRISYFYDLHGASMTVDTGCSTTLTALHLACQGLRNRESKTSIVTGANVILNPDMFVTMSSLGLLGPEGKSHTFDARANGYGRGEGIATVIIKRLDEALAAQDPIRCIIRGTALNQDGKTATLTSPSQTAQSDLIRACYRAAALDPNDTAFLAAHGTGTRTGDAVEIAAAAEVFGEKRLPDRPLWIGSLKTNIGHSEATSGLASVIQAALALEKGLIPPNINFKEPNEKLSQVSSAVKVPSTLEKWPLGSRVRRASVNNFGYGGANAHVILESGLTGSTQLANGNGHYETNGTTNGHKGANGTTNGHKGANGTTNGHNGTNGITNGHDITRGTIDYEPLESFVISLSAKEEAGTRSMMTNLGEYLRKNHVDDETKHFKSIAYTLGSHRSTFKWTAAKPITSLEELLAAAGGGQFQASRALERTRLGFVFTGQGAQWFAMGRELINTYPVFRKSLDRANGYLKEFGCEWSILDELSRDAETSNVNDMTLSPPLCTAVQISLVRLLESWGIVPTAVTGHSSGEIAAAYAAGALDFRSAMAVTYFRGEVGLACQDKIVGKGGMIAVGLGPEEAEDRIARVQSGKIVIAC",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that The acetyltransferase M4 appears to have a broad substrate selectivity for its acyl CoA substrate, allowing the in vitro synthesis of novel squalestatins (Probable); The citrate synthase-like protein R3 is involved in connecting the C-alpha-carbons of the hexaketide chain and oxaloacetate to afford the tricarboxylic acid unit ; The potential hydrolytic enzymes, M8 and M10, are in close proximity to pks2 and may participate in product release ; The biosynthesis of SQS1 requires several oxidative steps likely performed by oxidoreductases M1, R1 and R2 (Probable)2. The protein must be able to methyltransferase activity.3. A protein that is capable of catalyzing reactions within the Secondary metabolite biosynthesis pathway with high efficiency and specificity.4. The designed protein should contain one or more Carrier domains that are essential for its function. The designed protein sequence is ",
    "response#1": "IAVIGMGCRFPGGVNNPEQFWDLLCAGRSGIVRVPAQRWDADAYYCDDHTVPGTICSTEGGFLTSWQPDEFDAEFFSISPREAAAMDPQQRLLIEVAWEALEDAGVPQHTIRGTQTSVFVGVTAYDYMLTLAGRLRPVDLDAYIPTGNSANFAAGRLAYILGARGPAVVIDTACSSSLVAVHLACQSLRGRESDMALVGGTNLLLSPGPSIACSRWGMLSPEGRCKTFDASADGYVRGEGAAVVVLKRLDDAVRDGNRILAVVRGSAVNQDGASSGVTVPNGPAQQALLAKALTSSKLTAADIDYVEAHGTGTPLGDPIELDSLSKVFSDRAGSDQLVIGSVKTNLGHLEAAAGVAGLMKAVLAVHNGYIPRHLNFHQLTPHASEAASRLRIAADGIDWPTTGRPRRAGVSSFGVSGTNAHVVIEQAPEHSAAHTPQVLIGPDTVFRVARVSKLLTAIAILQLHDQGHISSLYDPINIYIPDLDPSTVQWGRITIWDLLNNVAGILDMYGYADIYTDFSARQRADLNLPPVPEDSLEGMPACQVDKSIPCDSAGLLSWLRSSRAVFEPHRINSNSNVGFSLLGILIERITGIDYERFISQTLIEPLHLNSTSFRPPHKNSGAVLQNDHTWNWDVGVNNPSVGLYSTPRDISTLLRWTLNESPSSLLNWFAPGFYAVGSHSLIGMPWNIFRTTAPLSIPNRPTTFNTVVGTLGPYTSVVVVMPEYDLAVSLMMNGALGHPL",
    "response#2": "IGKDTVFRVARVSKLLTAIAVLQLHDQGHVSSLYDPINDYIPDLEPSSVQWDRVTIWDLLNNVAGILDMCSYTLFDKRLNTNSDFTIDGYADIYMDFSARQKADLNLPPVSDAILEVMPACQVDKSIPCDSAGLLSWLRSSKAVFNPHQVNSNSNVGFSLLGILIERISGVKYEQFIHQTIIEPLQLNSTSFRPPHKDSGAVLQNDHTWSWDVGVNNPSVGLYSTPCDISTLLRWTLNKSPSSILNWFAPGFYAVGSHSLIGMPWNIFRTTTPLSIPNRPTTFNTVVGTLGPYTSVVVVMPEYDLAVSLMMNGALGHPETHTEWLLERNEPLAIVGSACRFAGGVSSPSKLWDLLSNPRDVRSEILKSRFNAENYYHPDSAYHGHSNIQHSYLLEEDVAAFDSQFFGIKPVEAKAIDPQQRLLMETVYEGLESAGLTIDSLRDSDTAVYVGLMCGDYEASLLRDLSTAPVYAATGIGRSILSNRVSYFFNWHGPSMTIDTACSSSLVAVHLAAQALRSGESRVALACGSNLLLGPENYIMESKLKMLSPDGRSKMWDKDANGYARGDGVAACVLKTLSAAIEDGDDIECIIRETGVNQDGATTGITMPSATAQQALIRSTYKKAGLDLSKVNDRPQFFEAHGTGTPAGDPIEAEAISKAFFDEQYGTMVAAEPLYVGSIKTILGHTEGTAGLAALLKASLALQHSVVPPNMLLNNLSDKVAPFTKNLEILKAPKAWPSVEAGQPRRASVNSFGFGGTNAHAILE",
    "response#3": "SEPIAVIGMGCRFPGGVNNPEQFWDLLCAGRSGIVRVPAQRWDADAYYCDDHTVPGTICSTEGGFLTSWQPDEFDAEFFSISPREAAAMDPQQRLLIEVAWEALEDAGVPQHTIRGTQTSVFVGVTAYDYMLTLAGRLRPVDLDAYIPTGNSANFAAGRLAYILGARGPAVVIDTACSSSLVAVHLACQSLRGRESDMALVGGTNLLLSPGPSIACSRWGMLSPEGRCKTFDASADGYVRGEGAAVVVLKRLDDAVRDGNRILAVVRGSAVNQDGASSGVTVPNGPAQQALLAKALTSSKLTAADIDYVEAHGTGTPLGDPIELDSLSKVFSDRAGSDQLVIGSVKTNLGHLEAAAGVAGLMKAVLAVHNGYIPRHLNFHQLTPHASEAASRLRIAADGIDWPTTGRPRRAGVSSFGVSGTNAHVVIEQAPAPPADAPVFTGQGAQYARMGAELLETSATARKIIQELQTCLEQLPEDLRPDFSLEEELRAAADSSRVLTGAFSFLSTVVQLLLVDLLKLAGVQFDAIVAHSSGEMAAAYAAGRLSARDAMCVAYFRGRFASKMESPNGADKKGAMLAAGMSEEDAATLCADEIFAGRVCVAAVNSSSSVTISGDEDAIDEFKLILDDENKFNRKLRVDRAYHSNHVVLS"
  },
  {
    "reference": "MDYYRKYTAAILAILCVCLHDLQSFPDREYTMQGCPECKLKENKLFSMLGDPIYQCAGCCFSQAYPTPARSKKTMLVPKNITSEASCCVAKAFTKVTVKGNVRVENHTECHCSTCYYHKS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH and follitropin/follicle stimulating hormone/FSH; These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways.2. A protein with follicle-stimulating hormone activity is needed to enhance negative regulation of organ growth, follicle-stimulating hormone secretion, cellular response to hormone stimulus in a specific context. The designed protein sequence is ",
    "response#1": "ERQPAXR",
    "response#2": "LFSLLPD",
    "response#3": "SVKGTR"
  },
  {
    "reference": "MSPPPPPPIWRQLSFSLLLGSFCIALESAAQGNSATDALNILLIIVDDLRPSLGCYGDKLVRSPNIDQLASHSVLFQNAFAQQAVCAPSRVSFLTGRRPDTTRLYDFNSYWRVHSGNFSTIPQYFKENGYVTMSVGKVFHPGISSNHSDDYPYSWSFPPYHPSSEKYENTKTCKGQDGKLHANLLCPVDVADVPEGTLPDKQSTEEAIRLLEKMKTSASPFFLAVGYHKPHIPFRYPKEFQKLYPLENITLAPDPHVPDSLPPVAYNPWMDIREREDVQALNISVPYGPIPEDFQRKIRQSYFASVSYLDTQVGHVLSALDDLRLAHNTIIAFTSDHGWALGEHGEWAKYSNFDVATRVPLMLYVPGRTAPLPAAGQKLFPYRDPFDPASDWMDAGRHTEDLVELVSLFPTLAGLAGLPVPPRCPIPSFHVELCREGQNLQKHLQLHDLEEEPDLFGNPRELIAYSQYPRPADFPQWNSDKPSLNDIKVMGYSIRTVDYRYTVWVGFDPSEFLANFSDIHAGELYFVDSDPLQDHNVYNDSQHGGLLHSLRP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein requires Ca(2+) to activate a critical catalytic site.2. The ideal protein would be one that plays a critical role in glycosaminoglycan catabolic process, heparan sulfate proteoglycan catabolic process, dermatan sulfate catabolic process through iduronate-2-sulfatase activity, sulfuric ester hydrolase activity, calcium ion binding within cytoplasm, lysosomal lumen, lysosome.3. A key design requirement for the protein is the optimization of the active site, Nucleophile to achieve high catalytic efficiency for Hydrolysis of the 2-sulfate groups of the L-iduronate 2-   sulfate units of dermatan sulfate, heparan sulfate and heparin..4. Include a signal peptide as an essential component of the protein.5. For general function, the protein need meet that Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate.6. The designed protein should have a binding site that is specific to the target Ca(2+) ligand. The designed protein sequence is ",
    "response#1": "MPHFWTGVGFCPAGRIDHEGISAALGDPALRLNLRLIAALPVGAVTHIRIHWLLELIQFWQYDPSGIPIYDFSKFDDFIDFLHEELRLSPVLEWMGNLGGVFSENPMQQSFYWEHLVKTTINHQIARHGSSRLVNWRYETWNEPDLRGYNKQNFTAHTFLDYVQAVRRGLSKAGNLDNQDGKVPLPMYRSLRGPAGLFKDSNHPLCWNLLELCSQRVVYCPIDILTFHRKGIEGTATEIVNGSLSLMAKIYEEYPNLKQLPVANDEADPVAGWSTSRDFQADVRYGITLISTVMQFWHAKLAGGPLSRLESISHDNAFLSYHPHEFTQRTLLAHFRMNETKPPHSQLVQKPVYAALGMLAKLGTRAADVEMVNMDTKHSVQVLRTVSGGLGGPGQYMATIFLSPEEAGPKMTAFHHKYTLNMSIANESAFVTELLVPKETDPYYIWQQAGSPAYPNATLREAMRRAQAPRRPVLGFP",
    "response#2": "MPHFWTGVGFCPAGRIDHEGISAALGDPALRLNLRLIAALPVGAVTHIRIHWLLELIQFWQYDPSGIPIYDFSKFDDFIDFLHEELRLSPVLEWMGNLGGVFSENPMQQSFYWEHLVKTTINHQIARHGSSRLVNWRYETWNEPDLRGYNKQNFTAHTFLDYVQAVRRGLSKAGNLDNQDGKVPLPMYRSLRGPAGLFKDSNHPLCWNLLELCSQRVVYCPIDILTFHRKGIEGTATEIVNGSLSLMAKIYEEYPNLKQLPVANDEADPVAGWSTSRDFQADVRYGITLISTVMQFWHAKLAGGPLSRLESISHDNAFLSYHPHEFTQRTLLAHFRMNETKPPHSQLVQKPVYAALGMLAKLGTRAADVEMVNMDTKHSVQVLRTVSGGLGGPGQYMATIFLSPEEAGPKMTAFHHKYTLNMSIANESAFVTELLVPKETDPYYIWQQAGSPAYPNATLREAMRRAQAPRLSIAEVTQREVFISWMEHPKSTQCLLSYEVWFKERDNLGRSADWMLISQGWHLPYPSFQYAPGDKGSVNGFYKVRGVDVFNETSPYSQIVEYLKASYIRNRSIRSVSIELDGAIYSMGLEPGLPPRNLTKRHQPAEGAPEDQEDKDGGSEFSGTGDLQEYVAPNLIKVTHRCYILENDTVQCDLDLYKSLQAWKDHKLHIDHEIETLQNKIKNLREVRGHLKKKRPEECDCSLSIAEVTQREVFISWMEHPKSTQCLLSYEVWFKERDNLGRSADWMLISQGWHLPYPSFQYAPGDKGSVNGFYKVRGVDVFNETSPYSQIVEYL",
    "response#3": "MPHFWTGVGFCPAGRIDHEGISAALGDPALRLNLRLIAALPVGAVTHIRIHWLLELIQFWQYDPSGIPIYDFSKFDDFIDFLHEELRLSPVLEWMGNLGGVFSENPMQQSFYWEHLVKTTINHQIARHGSSRLVNWRYETWNEPDLRGYNKQNFTAHTFLDYVQAVRRGLSKAGNLDNQDGKVPLPMYRSLRGPAGLFKDSNHPLCWNLLELCSQRVVYCPIDILTFHRKGIEGTATEIVNGSLSLMAKIYEEYPNLKQLPVANDEADPVAGWSTSRDFQADVRYGITLISTVMQFWHAKLAGGPLSRLESISHDNAFLSYHPHEFTQRTLLAHFRMNETKPPHSQLVQKPVYAALGMLAKLGTRAADVEMVNMDTKHSVQVLRTVSGGLGGPGQYMATIFLSPEEAGPKMTAFHHKYTLNMSIANESAFVTELLVPKETDPYYIWQQAGSPAYPNATLREAMRRAQAPRLA"
  },
  {
    "reference": "MKRIGGRKNDQLREVKITRDYTKYAEGSVLIEMGDTKVICNASIEEKVPPFLKNSGTGWITAEYSMLPRATHSRKIRESSKGKLEGRTQEIQRLIGRALRSVIDLKALGERTIWIDCDVIQADGGTRTASITGAFVAMADAVYKVYKSKGLKKMPITSFVSAVSVGIINGEKILDLCYEEDSNAQVDMNIVMTNKGEFVEIQGTGEEFPFTKEELDSLIDLGQIGNRNLIKAQREALGDISKLILGDEVEVVIATSNNHKLTEIGKILSEFNVKVLSLNDLGFKDIKIDETGSTFEENALIKAREVMKRTGKIAIADDSGLMVDILGGRPGIYSARFAGENSTDKQNNEKLLKLLNGYPLEKRSARFVSSIAVVFPDKREFTVKGTCEGKIALTPVGENGFGYDPLFIVDGYDKTFGELSSEVKNKISHRAKAMEKMKVELEKRI",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.2. The designed protein should be optimized for 3'-5'-exoribonuclease activity, phosphate-dependent exonuclease activity, ribonucleoside triphosphate phosphatase activity, nucleotide binding activity in order to improve tRNA processing, rRNA processing, purine nucleoside triphosphate catabolic process, rRNA catabolic process.3. The phosphate, Mg(2+), substrate binding site should be stable and able to withstand changes in environmental conditions.4. The presence of Mg(2+) is necessary for the protein to function in the desired environment.5. The designed protein should contain one or more Exoribonuclease phosphorolytic domains that are essential for its function.6. The desired protein should be able to perform phosphate + tRNA(n+1) = a ribonucleoside 5'-diphosphate +   tRNA(n) The designed protein sequence is ",
    "response#1": "MACSTFKSLISLARANSVSRGLTASRSFSSAQHSSSPMEKISVFGTRKMATRAEKLVEERNEAAEAAKPKVYFGNLPYNASTDQVVEFFNGFMLSRIDLPRDPKDPKRNAGYGFVTFDDHETYIEALKLNESEFKGRQITIKRRHDRPKADMEARAAEGTPNAKRPKMIIKNPSRVLMTRNLPNAVTQETLKTLCEGYSVDGDNRIVQDFELILDRNVALVRCDTHESATKAVLAFAKKNMNVYGNKVTLFYLDDDQANQIVERKEKETSGEGSMPEKTE",
    "response#2": "MIGKALLLSRKDFKSLLASKGMWIALGAIIALNVGLVFFNTFDVPSNDTYQGMMSSFISPEMNFLLGSVSSMELRSYFGAYQYLFIYGALVAIFYGYDLITKERDNGSLAFLRSQPVSRSAFITGKILAGLTASTIPLAVISLATMIAAYAAGFNVFSAENILRFVVFSVAASLYVSLFIAIGTLTGSQTRALASSIGIIGFFWIFGAIYNSAWPMIEYIHPIKYYFPIYALDPLEAVQTGTINTSFWFIELSTAILLSILAFWLFDNRDITA",
    "response#3": "MIDLPFDDLPPLDDFAARGADLRRTVRRDGLSDGYVFEGAAQPLLEHLIALAGRAAGERPAIVFADHAGSDWRAALGARVPAIPAADLHRMPAAAPARFADPARAARLAFTSGSTGLPKGVPRTFAQLREDLGAYGAALGIDAGERRIVANVPPYHIFGFTFGLLLPTLAGARLVPAPGFPVPAAIREIAASGATVLLATNSYLRLLVQAPGDALASLRLAVLGGDACPPALADAARARFPAAAIVTSYGSTEAGPLATSRPGDALRPDAAGRYAPGVEARLEQVDGGRVWVRSDMLAVAPGTVPGEWIETGDIAELRPDGALRVQGRAKRFAKIAGEMVSLEMVEQLAGDALPERAHALAVAAPGGGAKLVLFTSAPTLDRETLRRYARERGVPELAVPRDIRYLRELPLLGTGKPDFAAMRRLVEEGAA"
  },
  {
    "reference": "MEPVDPKLEPWKHPGSQPRTACTNCYCKKCCFHCQVCFLNKGLGISYGRKKRRQRRRAPEGDQAHQVSLSKQPSSQLRGDTTGPKE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation; Through its interaction with host TBP, Tat may also modulate transcription initiation; Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication; This binding increases Tat's affinity for a hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA) and allows Tat/P-TEFb complex to bind cooperatively to TAR RNA; Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to promote processive transcription elongation by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts; To ensure maximal activation of the LTR, Tat mediates nuclear translocation of NF-kappa-B by interacting with host RELA2. The protein's functional design necessitates the inclusion of a Nuclear localization signal, RNA-binding (TAR), and protein transduction, Cell attachment site motif.3. A protein that contributes to modulation by virus of host chromatin organization by functioning in extracellular region, host cell cytoplasm, host cell nucleolus with actinin binding, RNA-binding transcription regulator activity, protein serine/threonine phosphatase inhibitor activity. The designed protein sequence is ",
    "response#1": "MEPVDPRLEPWKHPGSQPKTACTPCYCKKCCFHCQVCFIKKGLGISYGRKKRRQRRRAPPDSQTNQASLSKQPTSQPRGDPTGPEESKKKVETETETDPEN",
    "response#2": "MEPVDPNLEPWNHPGSQPKTPCSKCYCKICCWHCQLCFLKKGLGISHGRKKRKHRRGTPQSSKDHQNPIPKQPLPTTRGNPTDPKESKKEVASKAETDPCA",
    "response#3": "MGGKWSKRSVGGWPAVRERMRRAEPAAEGVGAASRDLEKYGAITSSNTPTNAACAWLEAQEEEGEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQEILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVEPEKVEEANEGENNSLLHPMSLHGMEDPEKEVLVWKFDSRLAFHHMAKELHPEYYKDC"
  },
  {
    "reference": "MLILETVPLLRREVRRWRQEGKRIALVPTMGNLHDGHMALVNEAHARADVVIVSVFVNPMQFDRPDDLARYPRTLQEDCEQLNRHGVDLVFAPAAEEIYPQGVSQQTYVDVPALSSILEGASRPGHFRGVATIVSKLFNLVQPDLACFGEKDYQQLQLIRKLVADMGYGTEIVGVPIVRGEDGLALSSRNGYLDSDERRLAPRLYNIMMQLASQLENGERDLEALLEQTASRLRQAGFCPDELFIRDADTLGDVNVDTRTAIVLMAAWLGQARLIDNVRIAFNADA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be able to bind ATP ligand in a variety of conditions2. The protein must exhibit ATP binding. The designed protein sequence is ",
    "response#1": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#2": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#3": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR"
  },
  {
    "reference": "MFSIIIPIYNSENYLRYSIESVLNQSIGFKENIELILIDDGSVDSSPQICESFKNLYPNNIKIMKIENSGPSAARNCGLSNVSERSKFIGFLDSDDAFSQNALQSVYDFFCDSEHVNIAVLPVYYTGEKEGGHKLNNRFEKGTRVINILNDYKAIHFYIGGTFYRRHTLTSTVLFDESIKFWEDAIFFNQLLLKEKRYGAVAEGKYFYRKRKEQDSLVDRSWFNKKRYTYLLNECYMTLLMDSFNKYDIVLPYLQFLIVYHIKLFLYPNYRDVYKSVLDQQEQRVFVDDFIKVLKFIDPQFIKEQDMPMYYKEFMFHLLKENTEALENIKKERVLHSSCTVTSAKIKGLRLELTGHFINQYYEMKENDRIYIKYFKRLKKCKRKELKKTIEVWGYKLRDFRYAGFVVEIPIWAFAFDFVLKTPNDSLELNHVNIFKSLLTRVFKKR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Involved in the biosynthesis of galactosamine-containing minor teichoic acid, a non-essential cell wall polymer in B.subtilis 168..2. The protein design must be able to perform glycosyltransferase activity in order to contribute to cell wall organization, teichoic acid biosynthetic process.3. A protein that can modulate the overall flux of metabolites through the Cell wall biogenesis, poly(glucopyranosyl N- acetylgalactosamine 1-phosphate) teichoic acid biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "QGPCSLSGEVMISGSKNAALPILFSTLLSEEPIELKNIPELKDIDTTLNLLKQLGVKAKYQPPDSIMIDAKAVNHFCAPYELVKTMRASIWALGPLVARFGRGQVSLPGGCAIGARPVDLHIFGLKQLGAKIQIEEGYIKASVEGRLKGAHIVMDKVSVGATLTVMSAATLAEGTSIIENVAREPEIVDTANFLNILGANIQGAGTDKIVVEGVKRLRGGAYSIMPDRIETGTFLVAGAVSRGKVVCRHTQPATLEAVLAKLHEAGAQIEVGKDWISLDMKGKRPKAVNVRTTPYPGFPTDMQAQFTLLNIVATGTGVITETIFENRFMHVPELIRMGAQVKIESNTVICHGVERLSGAGVMATDLRASASLVLAGCIAQGITTVERIYHIDRGYECIENKLGLGR",
    "response#2": "TGGEKLQGSVSVSGAKNAALPLISATLLAKNEACLKNLPDVVDIKTLLSLLEMLGAKVQRIDPHTVKVNSSEILSTKATYDIVRKMRASILVLGPLLSRFGKCEVSLPGGCAIGARPVDLHLKAMEKMGAIVEIKGGYIVANAPKGLKGATIAFDKITVTGTENVVMAAALAKGVTKILNAAQEPEVVQLCEVLQAAGVEIKGIGSNELEIVGQEGEALEFKEVEVIPDRIEAGTYLCAGAMTNSSIRLERVVPEHLSAVTQKLEEIGFGIEYGENHLTLLPASKRRAFEIITTEYPGFPTDMQAQFMALATQCEGASIIEERLFENRFMHVSELQRLGAGIHLRGNTATVNGGTKLLCADVMATDLRASSALVLAALAAEGTTNIHRIYHLDRGYERLEEKLAALGARIERR",
    "response#3": "VEGYELDAEEREILAHPLVGGLILFMRNYHDPAQLRELVRQIREASHHRLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGLEEGGKLAQEAGWLMASEMIAMDIDISFAPVLDVGHISAAIGERAYHEDPQKALQIATRVIDGMHEAGMKTTGKHFPGHGAVTADSHKETPRDPRPQAEIRARDMSIFKSLISDNKLDAIMPAHVIYPDVDPRPASGSPHWLKTVLRQELGFNGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMILVCNNRKGAVSVLDNLSPIKAERVTG"
  },
  {
    "reference": "MSPEGEESHAEDNLYFHNTQMQSIHEETLAEESHAQAIQRSISRLSSFKHEDTDSESPIPVQKVIRQIKKTNTNTNTTKTKKRKLKVSKPRKNMSSSESVRQMYGSRKNGEQQGSIDGFLMSRFGEVDGCNESDRSSKLISQGEWESLSKFHEESNEKDKLLRKKIQRYYSEPQEELGEGCGMLTQASADPETKMTKEELESLYDLDSSTVFNQTTISAIGDMSEPTQVSEPCVVILSQTKVQSEASTQEDGADASVQEIKSTVPAQELFSTPEFVPAPLEQPVNKEMCSDQSIVVLEENVQSTQADDSDIVELISDSDPEPEEVSTKVQVLRSIYDSSGPVNDKQPSVASETVESDSTPIVSPVKTPQGTRMHVVQVASSNPSSPIKIVEENTEQQEEVFTDVESIYSTARTSFGTPKHTSPVILLESSDTDCDDNDNDVEPLSSMPMVKTVPKKRMRTTVLQVSAALKVQNYTDEKNNIKLRKIGDDIPVEVPTKDVEYEEIPDSQESVGGEGDNSVSIIEITREVHNNDYTGDESTDLFQVGLNKQDTCEDTSSPVVQIGGVENDILDSSLIEPVPEPESVPVPEEVQEQPQPTAIDKHTATQLREKFQLWGLKPVRGKEKMVEILQGISNFILPEHELLAVADKQELQSCIFRKLDAVIREGRAMYDRILSFEPIRLEELQSMLQSQDHYLELDVLRLYCDSSGITTTNA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be optimized for stability and solubility when expressed in Slx1-Slx4 complex where it will be involved in DNA replication, organelle organization.2. The protein design should emphasize Basic residues compositional bias. The designed protein sequence is ",
    "response#1": "LLLRLRAQTKQQLLEYKSMIDTNEEKTPEQIMQEKQIEVKIEELENEIEDVKSNIEMKSLALSRMKLSVALRDNMENMGPENCVLTDDMKHILKLQKLIMKSQEESSELEKKLLDVRKKRLQLKQASRSKLLEIQIEKNKQKEDVDKMENSEMIKTMKKKLQTEIKITTVVQHTFQGLILASKTNWAEDPALRETVLQLI",
    "response#2": "LLLRLRAQTKQQLLEYKSMIDTNEEKTPEQIMQEKQIEVKIEELENEIEDVKSNIEMKSLALSRMKLSVALRDNMENMGPENCVLTDDMKHILKLQKLIMKSQEESSELEKKLLDVRKKRLQLKQASRSKLLEIQIEKNKQKEDVDKMENSEMIKTMKKKLQTEIKITTVVQHTFQGLILASKTNWAEDPALRETVLQLV",
    "response#3": "LLLRLRAQTKQQLLEYKSMIDTNEEKTPEQIMQEKQIEVKIEELENEIEDVKSNIEMKSLALSRMKLSVALRDNMENMGPENCVLTDDMKHILKLQKLIMKSQEESSELEKKLLDVRKKRLQLKQASRSKLLEIQIEKNKQKEDVDKMENSEMIKTMKKKLQTEIKITTVVQHTFQGLILASKTNWAEDPALRETVLQLL"
  },
  {
    "reference": "MQYLAAYALVALSGKTPCKADVQAVLKAAGVAIELSRVDALFQELEGKSFDELMTEGRSKLVGSGSAAPAAAASTAGAAVAAAADAKKEASEEEADDDMGFGLFD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The ideal protein would be one that plays a critical role in cytoplasmic translational elongation through structural constituent of ribosome within cytosolic large ribosomal subunit.2. The design objective is to achieve a protein with turn characteristics.3. The protein must exhibit the following characteristics: Plays an important role in the elongation step of protein synthesis.. The designed protein sequence is ",
    "response#1": "MYAVVQVRGVVKTNHEIKDTLKMLRLHHVNHCVLVPDTPAYLGMIRKVKDFVAYGEVDKDTLATLLRTRGRLTGDEKLTDEYVRAHTPYADIDEFAAALCDGEMSFRDLVEIKPVLRLHPPRKGYKTIKRTFQQGGALGYYGPQINDLLYKMR",
    "response#2": "MYAVVQVRGVVKTNHEIKDTLKMLRLHHVNHCVLVPDTPAYLGMIRKVKDFVAYGEVDKDTLATLLRTRGRLTGDEKLTDEYVRAHTPYADIDEFAAALCDGEMSFRDLVEIKPVLRLHPPRKGYKTIKRTFQQGGALGYYGPQINDLLYKMR",
    "response#3": "MYAVVQVRGVVKTNHEIKDTLKMLRLHHVNHCVLVPDTPAYLGMIRKVKDFVAYGEVDKDTLATLLRTRGRLTGDEKLTDEYVRAHTPYADIDEFAAALCDGEMSFRDLVEIKPVLRLHPPRKGYKTIKRTFQQGGALGYYGPQINDLLYKMK"
  },
  {
    "reference": "MTLFRSHLRFLFKPRFLYFQSPTGQSSRPFSTSQILRTALDMPPPPVDTTQRLAKLRELMKQNKVDVYIVPSEDSHQSEYIAPCDGRRAFISGFTGSAGCAIVSMSKAALSTDGRYFSQAAKQLDANWKLLKRGVEGVPTWEEWTAEQAENGKVVGVDPSLITAADARKLSQTLKATGGSLVGIDQNLIDIVWGDERPARPVTTITVQPVELAGKPFEEKVEALRKELATKKRSAMVISAEIYVDDSRLSPEARKQLEGKVVLKPYDAIFQASKVLAESKASASDGAASGKFLLSNKASWSLSLALGGEQNVDEVRSPITDAKAIKNDVELEGFRKCHIRDGAALIEYFAWLENALIKEGAKLDEVDGADKLYEIRKKYDLFVGNSFDTISSTGANGAIIHYKPEKSTCSVIDPKAMYLCDSGGQYKDGTTDTTRTLHFGEPTEFQKKAYALVLKGHISIDNAIFPKGTTGYAIDSFARQHLWREGLDYLHGTGHGVGSFLNVHEGPMGIGSRAQYAEVPLSAKNVLSNEPGYYEDGNFGIRLENLVICKEVETTHKFGDKPFLGFEYITMVPFCQKLLDASLLTEAERKWVNDYHAKVWEKTSPFFEKDELTLNWLKRETQPI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that can perform metal ion binding in cellular anatomical entity to support proteolysis.2. The protein must exhibit the following characteristics: Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides..3. The designed protein should be able to compete with endogenous proteins or inhibitors for Mn(2+) binding. The designed protein sequence is ",
    "response#1": "FTRNLGNLPPNICHPTFLGEQAKNLGKEFKDLKVEVLDEKKIKSLGMGSFYAVGQGSAQPPRLIVMQYNGGKKSEKPYALVGKGITFDTGGISLKPGAGMDEMKYDMGGAASVFGTLRAVLELKLPINLVCILACAENMPSGTASRPGDIVTTMSGQTVEILNTDAEGRLVLCDALTYSERFKPQAVIDIATLTGACVVALGAHTSGLLGNNDELIEQLLSAGKAADDRAWQLPLFDEYQEQLDSPFADIANIGGPKAGTITAACFLSRFTKNLNWAHLDIAGTAWTSGGKDKGATGRPVPLFISSFTGSAGCAIVSMSKAALSTDGRYFSQAAKQLDSNWTLLKRGVEGVPTWEEWTAEQAENGKVVGVDPSLITAGENLHYTPLTSVVVTNCSYVIADAA",
    "response#2": "LAREMVAAPANEVTPLTFTEIATELAQTYGLELEVLGQTECEALGMGAFLGVAKASELPPQFIHLTYRPANPVKKLAIIGKSLTFDSGGLNIKGAGSGIETMKMDMGGGGATLGAAKAIAQLKPNVEIHFICAATENMISGTAMHPGDILTASNGKTIEVNNTDAEGRLTLADALVFAEKLGVEAIVDLATLTGACIVALGDDIGGLWSPNQELADELKVAADKAGEKFWQMPMESKYFEGLKSPIADMKNTGPRSGGSITAALFLQQFIKETPWAHLDIAGPVWTDKQNGVHNAGATGYPVRTLVR",
    "response#3": "FTRNLGNLPPNICHPTFLGEQAKNLGKEFKDLKVEVLDEKKIKSLGMGSFYAVGQGSAQPPRLIVMQYNGGKKSEKPYALVGKGITFDTGGISLKPGAGMDEMKYDMGGAASVFGTLRAVLELKLPINLVCILACAENMPSGTASRPGDIVTTMSGQTVEILNTDAEGRLVLCDALTYSERFKPQAVIDIATLTGACVVALGAHTSGLLGNNDELIEQLLSAGKAADDRAWQLPLFDEYQEQLDSPFADIANIGGPKAGTITAACFLSRFTKNLNWAHLDIAGTAWTSGGKDKGATGRPVPLIR"
  },
  {
    "reference": "MACSIVQFCYFQDLQAARDFLFPHLREEILSGALRRDPSKSTDWEDDGWGAWEENEPQEPEEEGNTCKTQKTSWLQDCVLSLSPTNDLMVIAREQKAVFLVPKWKYSDKGKEEMQFAVGWSGSLNVEEGECVTSALCIPLASQKRSSTGRPDWTCIVVGFTSGYVRFYTENGVLLLAQLLNEDPVLQLKCRTYEIPRHPGVTEQNEELSILYPAAIVTIDGFSLFQSLRACRNQVAKAAASGNENIQPPPLAYKKWGLQDIDTIIDHASVGIMTLSPFDQMKTASNIGGFNAAIKNSPPAMSQYITVGSNPFTGFFYALEGSTQPLLSHVALAVASKLTSALFNAASGWLGWKSKHEEEAVQKQKPKVEPATPLAVRFGLPDSRRHGESICLSPCNTLAAVTDDFGRVILLDVARGIAIRMWKGYRDAQIGWIQTVEDLHERVPEKADFSPFGNSQGPSRVAQFLVIYAPRRGILEVWSTQQGPRVGAFNVGKHCRLLYPGYKIMGLNNVTSQSWQPQTYQICLVDPVSGSVKTVNVPFHLALSDKKSERAKDMHLVKKLAALLKTKSPNLDLVETEIKELILDIKYPATKKQALESILASERLPFSCLRNITQTLMDTLKSQELESVDEGLLQFCANKLKLLQLYESVSQLNSLDFHLDTPFSDNDLALLLRLDEKELLKLQALLEKYKQENTRTNVRFSDDKDGVLPVKTFLEYLEYEKDVLNIKKISEEEYVALGSFFFWKCLHGESSTEDMCHTLESAGLSPQL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have enzyme activator activity, enzyme regulator activity within protein-containing complex to regulate intracellular protein transport.2. For general function, the protein need meet that Required for normal eye and brain development ; Required for recruiting and activating RAB18 at the endoplasmic reticulum (ER) membrane where it maintains proper ER structure The designed protein sequence is ",
    "response#1": "MTGAPVIFVPGNAGSYKQVRSLASVALRKAMSNDAGIHLDYYTIDYDEELSALYGGYLHRQQSYLKLCIRTILSIYEGRTEQPSIVLIGHSMGGKLAQSVLVDPAIGQHINTIISISTPLDQPVLNLDTQLEEFYDQTDAVLSKLRTATVPTMTTNVCDSLHQRPPSVQRMASQDSSARLDNVLLISTGGGNRDLLVRPGLTSSRFNDLHAMTSAIPKVSLSCDHLSAVWCLQFMQAINRFLFSR",
    "response#2": "MTGAPVIFVPGNAGSYKQVRSLASVALRKAMSNDAGIHLDYYTIDYDEELSALYGGYLHRQQSYLKLCIRTILSIYEGRTEQPSIVLIGHSMGGKLAQSVLVDPAIGQHINTIISISTPLDQPVLNLDTQLEEFYDQTDAVLSKLRTATVPTMTTNVCDSLHQRPPSVQRMASQDSSARLDNVLLISTGGGNRDLLVRPGLTSSRFNDLHAMTSAIPKVSLSCDHLSAVWCLQFMQAINRFLFST",
    "response#3": "MTGAPVIFVPGNAGSYKQVRSLASVALRKAMSNDAGIHLDYYTIDYDEELSALYGGYLHRQQSYLKLCIRTILSIYEGRTEQPSIVLIGHSMGGKLAQSVLVDPAIGQHINTIISISTPLDQPVLNLDTQLEEFYDQTDAVLSKLRTATVPTMTTNVCDSLHQRPPSVQRMASQDSSARLDNVLLISTGGGNRDLLVRPGLTSSRFNDLHAMTSAIPKVSLSCDHLSAVWCLQFMQAINRFLFSR"
  },
  {
    "reference": "MRRAIFPGTFDPFTIGHYSVVKRALTFMDEVVIGIGINENKKTWFPTEKRVEMIEKLFADDPRVKVDAYDCLTIDFARAKEAQFIVRGIRTVHDFEYEETIADINRKLAGIETILLFTEPELTSISSTIVRELLQFGKDVTPFLPEGMKID",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis pathway to avoid interference with native metabolic processes.2. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining pantetheine-phosphate adenylyltransferase activity, ATP binding.3. The protein should have a low Km value for (R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA +   diphosphate.4. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.5. The protein should be able to bind ATP, substrate ligand in a variety of conditions6. For general function, the protein need meet that Reversibly transfers an adenylyl group from ATP to 4'- phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate. The designed protein sequence is ",
    "response#1": "MQLFPGSFDPITNGHVETAKKAAQMFDKVFVVAMTNTSKKYLFTAEERTAFAKDALKNISNIEVLEKPEELTVKLAHELKANVIVRGVRNSADFLYEQEIAGINKRLAPDINTVLLFSSPDNSFVASSMIKKPLY",
    "response#2": "MLFPGSFDPITNGHVETAKKAAQMFDKVFVVAMTNTSKKYLFTAEERTAFAKDALKNISNIEVLEKPEELTVKLAHELKANVIVRGVRNSADFLYEQEIAGINKRLAPDINTVLLFSSPDNSFVASSMIKESDPQDLENIVKIYPGTFDPVTNGHADLIERAAKLFKHVVIGIAANPSKKPRFTLEERVALLQLVTSHLDNVEVVGFSGLLVDFAKEQKASVLVRGLRAVSDFEYEFQLANMNRRLSSDLESVFLTPAEENSFISSTLVKESDPQDLENIVKYVYPGTFDPITLGHMDIIRRGAKLVDRLVIGVTTNITKSPLFDDDERIAMVKREVAAIEGDIQVVGFNSLLMDFAQREGATVIVRGLRAVADFEYEYQMAGMNQQLNDRIETVFLMADVGLQPIASRLVKESDPQDLENIVKNLYPGTFDPITLGHQEIIERGSFLCDRLYIGVAVGHHKKTLFSLSERCELVNGVLADLTLHCPVEVVPYCGLLATLYQKLHANILIRGLRSAGDFEYERQLFYANQHLNAQIETVFLLPAPQYSFISSTLVRESDPKDLENIVKHVKSL",
    "response#3": "LYPGSFDPVTNGHLDVVRHAVALCDKLVVAIGIHPGKKPLFTTEERLAMVERVFGPVAKAAGCDFGCTTYDNLTVTAAEKVGATIMIRGLRDGTDLDYEMQIAGMNETMAPAIHTVFLPASVGVRPITATLVREPEA"
  },
  {
    "reference": "MQTLQTTSLRVVDNKLWILDQQALPQQKNWLPADNTEALVGHIHALRVRGAPLIGLSASLLLALLAEHGMTRDALGQALEVLRAARPTAVNLMNNLDRMKQALTQPDFVAALGAEALRLVQEDRELCERIARAGSELVKPGSRLLTHCNTGGLATAGVGTALGVIARAHEAGKVANVWVDETRPLLQGGRLTAWELGELGVPYQLITDSMAASLMAQGQVDAVWVGADRIAANGDVANKIGTYSLAVLAKFHNVPFYVAAPQTTLDPACPNGAAIPIEQRAAAEVTGVAGSFGAVQWAPEDARVYNPAFDVTPAALISGWVLDSGVVTPEDVANGRFAG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be designed to catalyze the following reaction S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-   D-ribulose 1-phosphate.2. The protein design must be focused on enhancing the efficiency of L-methionine salvage from methylthioadenosine.3. For general function, the protein need meet that Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P). The designed protein sequence is ",
    "response#1": "LARLAARVAREFDGCFDRVLAVESRGFVLGAALAASTGLPLTLARKPGKLPGPVYEAGYELEYGHDRLELQKGALAPDERVLCVDDVLATGGTLAATARLVALSGARVAGLVALVGLEGLGGAQRLSE",
    "response#2": "LARLAARVAREFDGCFDRVLAVESRGFVLGAALAASTGLPLTLARKPGKLPGPVYEAGYELEYGHDRLELQKGALAPDERVLCVDDVLATGGTLAATARLVALSGARVAGLVALVGLEGLGGAQRLSE",
    "response#3": "LARLAARVAREFDGCFDRVLAVESRGFVLGAALAASTGLPLTLARKPGKLPGPVYEAGYELEYGHDRLELQKGALAPDERVLCVDDVLATGGTLAATARLVALSGARVAGLVALL"
  },
  {
    "reference": "MSEPTGQMRFNESLANYTSWRVGGLAERFYQPADKADLIRFVQNLPENEPLFWMGLGSNLLVRDGGIRGTVINTKNRLKVMQCVDDETIYVEAGVPCALVAKFCAEQGWVGAEFLAGIPGTMGGALKMNAGAFGGETWKIVQKIEMLNRRGKVFTKSAQEFESSYRHVNLKADEWFLASTFKLEKGDTSESQQNIKALLVKRANSQPTNQPSCGSVFKNPTGDYAARLIEAAGLKGFKIGGAQVSEKHANFIINTGNATAADIEQLMTFVQVQIAQKYGINLQTEVCIVGEKLNAKHIERAQDFGMVAVLMGGNSAERDVSLRSGAAVHAALVARGVNALAVDVTGNVIDSLANLDVDRVFNIIHGRGGEDGVLQGVLDVLKLPYTGSGVLASALAMDKLRTKLCWRGANLPTPNWFVLKTENDIDACIEVLGFPIIVKPALEGSSIGMSKAKNRAELVVALEIALACNCEVYAEAWVTGDEYTVSILQGQALPIIRLETPHEFYDYEAKYNSTTTQYHCPCGLNAEQELQLQQLALQASGIIRVEGWARVDVFIDQNGQAQLIEINTVPGMTEHSLVPMAAKQVGIDFNTLVWRILETSIRK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The stability of the protein-Mn(2+) complex should be optimized to maximize enzymatic activity.2. A protein that can perform ATP binding, D-alanine-D-alanine ligase activity in cytoplasm to support cell wall organization, cell cycle.3. The designed protein must have a active site active site that is highly conserved among related enzymes.4. The protein should be optimized for activity in Cell wall biogenesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. The protein contains novel ATP-grasp domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "LWKGFGLPIADMEIVTKNTALSLEPQAIVARLGLPLMVKPSREGSSVGLTKVDSADKLKSAVDLALKFDDIVLIEEWLSGDELTVPVLGDEVLPAVQIVPEGEFYDYNAKYISDNTRYICPMPMSDERWDELKNLVKRAYEAVGCRGWSRIDVMTDSEGNFRLIEVNTTPGMTSHSLFPKSAATVGYSFEKLVEKILS",
    "response#2": "FRSLGLAVADYRVFPRASAGSIGVGDLPFGLPCVVKPAGEGSSVGVHLVNEAAELGPACRDAASHAGDVIVERYVKGTEVDVAVLDGKALGAIEIVPANAFYDYAAKYTAGTTKYFYPARLPEAHVRAVMEAAEAAHRGIGCSGVTRVDFIVAGDGTPYILEVNTLPGMTATSLVPKIAAGLGLSFPDLCERIADAGVEVAGFMSVTSADYAADPQAICNAVTERFAFPVFVKPANLGSSVGISKVHNAAELRPALDKACALDAKVLVEETITGREVEVAVLGNDDPIASVPGEIIPGSDFYDFEDKYVKSDAKLVIPADLPGEVSAAVRNAALTVFKALGCSGMARVDFFVEHGTNRIILNEINTIPGFTDISMYPMLMSASGVEFDELISRLADAGVEVAGFMSVTSADYAADPQAICNAVTERFAFPVFVKPANLGSSVGISKVHNAAELRPALDKACALDAKVLVEETITGREVEVAVLGNDDPIASVPGEIIPGSDFYDFEDKYVKSDAKLVIPADLPGEVSAAVRNAALTVFKALGCSGMARVDFFVEHGTNRIILNEINTIPGFTDISMYPMLMSASGVEFDELISRLYGMEEGRVIWRSEGLPTPEWRQVDSAAATSEAFAALGSPMIVKPDREGSTIGLTKVTQIEQCGAAYALAARHDAMVLCEQFVKGDEVTIPLLGSGAGAHALPVIRIVAPDGNYDYQNKYFTDTTQYLVPANLPAGEEAHIQQLALKAYQVLGCRGWARVDVMIDARTRAPFLLEINTSPGMTPHSLVPMAAKAAGVSYPALCLE",
    "response#3": "FRSLGLAVADYRVFPRASAGSIGVGDLPFGLPCVVKPAGEGSSVGVHLVNEAAELGPACRDAASHAGDVIVERYVKGTEVDVAVLDGKALGAIEIVPANAFYDYAAKYTAGTTKYFYPARLPEAHVRAVMEAAEAAHRGIGCSGVTRVDFIVAGDGTPYILEVNTLPGMTATSLVPKIAAGLGLSFPDLCERIL"
  },
  {
    "reference": "MGFWSALFGRGHSPALDGIEARAWEPYDPSIYNLGAVAASGETVTPHDALQVSAVFASVRLLSETIATLPLSTYSKRGGSRKEIVTPEWLDYPNAEPGGMGRIDILSQTVLSLLLQGNAFLAVRWQGPNIVGLDVLDPTKIHVHMVMVDGLRRKVFEAYDIDADGNEVLLGWFTPRDVLHIPGMMLPGDFVGCSPISYARESIGLALAAQKYGSKFFANGAMPGAVVEVPGTMSEEGLARAREAWRAANSGVDNAHRVALLTEGAKFSKVAMSPDEAQFLQTRQFQVPEIARIFGVPPHLISDATNSTSWGSGLAEQNIAFTMFSLRPWLERIEAGFNRLLFAETADRFRFVKFNLDEIKRGAPKERMELWSLGLQNGIYSIDEVRAAEDMTPLPDGLGEKYRVPLNLGEVGEEPEPEPAPAPPAIEPPAEEPDEEPEPEGKPDDEGATEEDDEDDA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: The portal protein multimerizes as a single ring-shaped homododecamer arranged around a central channel; This portal plays critical roles in head assembly, genome packaging, neck/tail attachment, and genome ejection.2. Target a Acidic residues compositional bias in the protein's composition for improved properties.3. A protein that contributes to proteolysis, viral genome ejection through host cell envelope, long flexible tail mechanism by functioning in viral capsid with peptidase activity, DNA binding. The designed protein sequence is ",
    "response#1": "GRK",
    "response#2": "LHHHHHHPDAF",
    "response#3": "GFPVL"
  },
  {
    "reference": "MATSTTGSTLLQPLSNAVRLPVDQVNFVVCQLFALLAAIWFRTYLHSSKTSPFIRHVVATLLGLYLALFCFGWYALHFVIQSGISYYLMIIIGVENMHKYCFVFALGYLTVCQITRVYIFDYGQYSADFSGPMMIITQKITSLAFEIHDGMFRKNEDLTPSQRCLAVRRMPSLLEYLSYNCNFMGILAGPLCSYKDYITFIEGRSYQLQQSEANGKEDTKYEQTDPSPNIAVAQKLLICGLSLLFHMTITKTLPVEYNIDENFRATASWPVRVFYLYLSLMAARPKYYFAWTLADAINNAAGFGFRGYDKNGVTRWDLISNLRIQQIESSTSFKMFLDNWNIQTALWLKRVCYERATFSPTIQTFILSAIWHGVYPGYYLTFLTGVLMTLAARAIRNNIRHYFVESPAVKLCYDIITWMTTQVAISYTVVPFVLLSVKPSFTFYSSCYFCLHIASILVLLVFPLKRTQKGNKQHESIQPVWSKKLEEENLLQKNSYSTTNNSFSQKEEITCRYQALKQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be optimized for activity in Lipid metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The designed protein must be able to perform 1-acylglycerophosphoethanolamine O-acyltransferase activity, 1-acylglycerol-3-phosphate O-acyltransferase activity, 1-acylglycerophosphoserine O-acyltransferase activity under specific conditions. The designed protein sequence is ",
    "response#1": "RFEVKGLENFDVEGPAIIVSNHQSILDMMGLMEVLPDDCVQVGKKELMYAGTVGLIIYLGGVIFINRKSTSSAKMVMAEVAKTMATDNVKVWVYPEGTRNCTGDLHPFKKGAFHLAVQAQVI",
    "response#2": "RFEVKGLENFDVEGPAIIVSNHQSILDMMGLMEVLPDDCVQVGKKELMYAGTVGLIIYLGGVIFINRKSTSSAKMVMAEVAKTMATDNVKVWVYPEGTRNCTGDLHPFKKGAFHLAVQAQVR",
    "response#3": "AIIVSNHQSILDMMGLMEVLPDDCVQVGKKELMYAGTVGLIIYLGGVIFINRKSTSSAKMVMAEVAKTMATDNVKVWVYPEGTRNCTGDLHPFKKGAFHLAVQAQVTAG"
  },
  {
    "reference": "MASTPEFQHTVGKTASFSGTSLHTGEKVSLKLHPAPVDHGIKFKRKDLQDEPTIDARLENLKTVERATTIGEGSVRVHTVEHVLATLSGMGVDNAIVEMDANEPPIGDGSAQPYVDLIKRAGVTAQEEPRKFFDVREPVHVESKTGALLVLLPDEKFRISCTQAGPDNRFAQYLSMEVTPAVFEREIAPARTFVFYEDVEPLMEKNLIKGGSLENAIVVRGDAVLSKEPLRFPDEFVRHKMLDIIGDLALIGRRIRGHLVAVKPGHAVNADLARALTREQTRRSALSVTRAVPRGDGGFDTDEVMQILPHRFPFLMVDRIISFESETKCIGIKTVTINEPFFQGHFPGHPVMPGVMQVEAMAQVASILLFKLAKTTSRIGYFMSADGVKFRKPVFPGDTIFIHAELTKSRGERLAKAKCHCVVNDAVVSEGELMFTFLDK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must exhibit the following characteristics: Involved in unsaturated fatty acids biosynthesis; Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs..3. The protein design should consider the endogenous proteins and enzymes present in the Glycolipid biosynthesis, lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 2/6 pathway to avoid interference with native metabolic processes.4. A key design requirement for the protein is the optimization of the Proton donor to achieve high catalytic efficiency for a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine +   H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate. The designed protein sequence is ",
    "response#1": "MTGKGRPLAPLRGLSGGRPPAXLPPLXSXAXLDDRXXLPXGVAXPXPRLAXAXGXRXGXLFXGXHXSXPSXGXGAXGXPXGXGXGXXT",
    "response#2": "MASSEAATPLGHRLRTWGARHALEPSLPALMEGVSTDSRRVAPGHLFVAVRGAHFDGHRFVADALARGAVAALVEAPLPGGDLPLLVVRDSRRALAELAAAWFDHPTEELWLVGVTGTNGKTTTTFLLEQALRASGRPCGLLGTIGYGVPGALELANTTPDPVDLQRFLARLRAAGAEAAAMEVSSHALGQGRVRGCRFRVAVFTNLTRDHLDYHGTMEAYRDAKASLFEGLDPGAVAVINADDPAARAFLGATRAEVLTYGVEAGSEVRAARVRLGPGGTAFDLVTPRGERPVRLPLVGRFNVYNALAAAAACLALGVPLEDAAAAVPALPQVP",
    "response#3": "MNGLTVKELAARVNARLHGDGDLAXVXVTXDSRAXAAGSLFVAVPGALHDGRQFIPDVLARGAAAVIVSEDVNVEGXGXXEXPXLTGXPXRAAGLXAAXFYGRPSEXLXVXAXVAXA"
  },
  {
    "reference": "MAIDEELAEANLVLYGGTCPNCGGLISDERLSRGAPCSDCLPSVEGGFLGLSQVVGELRRLRNLKNLKEFANALEEEKKLCEFFRSCVGSEPWSIQKLWIRRISKKSSFAMIAPTGIGKTTLGLVAALYYSFKGRKSYFIVPTTTLVMQAERKLIEFLNRKGSAASILAIHSKLRKREREERERRLEDPRGFDILITTSRYFVKNYDKISKHDFTFIFVDDVDAVLRGSKAINYLLELMGFTDEDIEDALKIIKLKRELGVRSESSELLEELRKLRSRLEKRKNTNKVLIIASATGNPRGLKVRLFRELIGFEIGARPELIRNIEDTYLKVRDLEEAVEEVTRLVRESLGGGGLIYVPVDLGIGFAEELASRLRSRGVVAEAMHSKKIETLEDFNSGSVDVLVGVATYYGVLVRGIDLPTRIRYAIFVDVPRHKINLRLKKLSATDVLRLMPLLRDVIVNPDDRSFLENAFVKLRRVLRRSGAYFLKVIDEVLEGVRGPQTASEKLFMEVYAKVHELLKDEAVVEGLVKHPEVVVVREGEALYVLIPDAPTYIQASGRTSRLYLGGVSKGLSVVVTWNEKLLRALERRLKLLTEDFVFKNLLEIDLNQVINEINRTRDEILAIGRGELIHDLGRRIELKTALMVVESPNKARTIARMFGKPSVKEYGRLRVYEVNIGNYTLLITASGGHVYELITDQGVEGVDPANYVYGVLYREGINKIPFIPVFAPIKRCMRCGHQFATLEDRTACPLCGSEEVLSSSDIIQSLRE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be able to bind ATP ligand in a variety of conditions2. The designed protein should have helicase activity to facilitate DNA topological change.3. The protein must exhibit the following characteristics: It cleaves transiently a single DNA strand and remains covalently bound to the 5' DNA end through a tyrosine residue; May be involved in rewinding the DNA strands in the regions of the chromosome that have opened up to allow transcription or replication..4. The protein contains novel Helicase ATP-binding, Toprim domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "LGESFAIVAPTGVGKTTLLLVYGTYMASKGKRVYVLVPTENLLLQTRQKLASMIERLGKDVRAVSYSSRDCKSARERALEHVVNGSFDILLTTTSFLSRRFGLLEGMKFDVIIIDDADSLLKKSKNIDRVLLLLGFSSSDIEAAYKLLKAKGELALLRAVGAEDRLEKKLLEVEELQLRVNEALATKKIGQVIIASATGRQVGLKPKLFRELLGFEIGQSGVGAAISDKLGRIGIHNLQDLLFHLPIRYEDRTRITPIHDLRPDAYATIEGLVQTCEVQFGKRPILNVSLSDGTSKIMLRFFNFNAGMKNSLQPGARVKAFGEVKRGRFMAEIHHPEYQIIRDNAPLILEETLTPIYSTTEGIKQNSLRKLTDQALAKRIFQRKSFSIIAPTGVGKTVFGLMMSLYLGKNDGKSYLIFPTTPLVIQMCEKLKQFNAKANAIEDDGIICIHAGMKQSEKNAAIEKLMEGKFRVFISTSKYMINKNNELSRLGLSFIFIDDVDSVLKSKKSIDSTLKLMGFNEEIIETALKVIKLRRKIAYMGKEASEKDLRELEKLERKVEKSKDKVKSVLVVSSATGKARGSRALLFRELLGFQI",
    "response#2": "VGGYPWNLQISWAYRIAQNQSFAMIAPTGVGKTTFGLITALFLSYKYNRKVYIVVPTSILVSHYYEKLLEYMNRAGIVVEVIAIHSKMSAKKRLELENGIKEGKFDILITTSNYLQRNFIDIFKPLIDKGIKINFIFADDVDAIMKGSKAIEYILLLMGFSEEDMQLGYKLAGLRLRLTRCEGVRERGYSEYCKEEGMPITEMFNEYQKKLAEKRANAGILVVSSATGKARGRRIKLFRELLGFIIGAGVEIGSSGVGAAISDKLGRIGIHNLQDLLFHLPIRYEDRTRITPIHDLRPDAYATIEGLVQTCEVQFGKRPILNVSLSDGTSKIMLRFFNFNAGMKNSLQPGARVKAFGEVKRGRFMAEIHHPEYQIIRDNAPLILEETLTPIYSTTEGIKQNSLRKLTDQALAKRVFMNQSFAIVAPTGVGKTTFGLVMSLFLKGRVLAIFPTRLLAQQAGDRLSELAQKVGVNKKILIYQSKKNIREQFLNGDWDILLGTNMFLHKNFENLINFKFKLIFIDDIDSFLKRGKNVDYLFKLLGFSGEEIKLALKENKTQRDYDRLARIRKRKRDTVLIVSSATLKPRGKRAYLFRNLLGFDDSGVGAAISDKLGRIGIHNLQDLLFHLPIRYEDRTRITPIHDLRPDAYATIEGLVQTCEVQFGKRPILNVSLSDGTSKIMLRFFNFNAGMKNSLQPGARVKAFGEVKRGRFMAEIHHPEYQIIRDNAPLILEETLTPIYSTTEGIKQNSLRKLTDQALHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLIQLFGNTMNVALLTGSVKGKKRRLLLEQLENGTIDCLIGTHALIQDDVVFNNVGLVITDEQHRFGVNQRQILREKGAMTNVLFMTATPIPR",
    "response#3": "LRRSFALLAPTGVGKTTFGLVTALFLSGEGRKSYIILPTKLLMEQVHRKLTEWGLSEEELLVAGGTSEKKRRELKERISEGRFRVLVTTSMYLYRNHDIIPKGFDFIFIDDVDSFLKTARNIDKVLHLLGFSEEDLKKAYTLIKVKEKRRKEEEDWELIRKLSEEVKRLQQKAGGVLVVSSATGNPRSSRIKLFRELLGFEVGRGDSFSIVAPTGTGKTTFGIVAALYLACVKKAKSYILVPTTTLVAQIVEKANTLAKNIGCTPIVVGFHSKMKRSERENALNMIMSGGFDILVSTIAFARRYADILSNYKFKFVLVDDVDAVLKSGRSIDAVLKIVGFSRDHLEKATKLLSLQREIARILESASEEEFREKSSIKSKLANIQNDIEAYKRELLEMRGKVAQVIFSSATGRPRGSRVRLFRALLGFEVGGRGDIGLRNIINVPGRDFPVDAYVSGVGAAISDKLGRIGIHNLQDLLFHLPIRYEDRTRITPIHDLRPDAYATIEGLVQTCEVQFGKRPILNVSLSDGTSKIMLRFFNFNAGMKNSLQPGARVKAFGEVKRGRFMAEIHHPEYQIIRDNAPLILEETLTPIYSTTEGIKQNSLRKLTDQALLVQGDVGSGKTLVAALAALLAIDNGKQVALMAPTEILAEQHATNFRRWFESLGIEVGWLAGKVKGKARQTELEKIRTGQVQMVVGTHALFQDEVEFSDLALVIVDEQHRFGVHQRLMLREKGKQADHYPHQLIMTATPIPRTLETLSEKRQAYWVCTLIDESEVLEAQAAEAIAEDLRKILPHLRIGLVHGRMKPAEKQDIMQAFKQAEIDLLVATTVIEVGVDVPNASLMIIENAERLGLSQLHQLRGRVGRGTTASFCVLMYKPPLGKISQKRLQVLRDTQDGFVISEKDL"
  },
  {
    "reference": "MQRRAIVLAAGKGTRMKSKKYKVLHEVAGKSMIEHVVDNVKRSGVEQLVTIVGHGAESVKETLGDASLYSFQEEQLGTAHAVKMASEHLQDKQGTTLVVCGDTPLITTETLKALVDHHEQNQAQATVLSATAPNPFGYGRILRDSDNHLVKIVEQKDATENEREINEISSGIFAFDNQVLFDKLNHVKNDNVQSEYYLPDVLSLILEDKGSVEVYHTDDFDEIMGVNDRLMLSEAESAFRQRINEYHMKNGVTIIDPATTYIGVNVKIGEDTVIEPGVKLVGYSEIGEDVIIGQYTEITNSSIGSNVTIKQSVINESNVDDYATVGPFAQLRPGADLGKKVKVGNFVEVKKSIIKDGAKVPHLSYIGDAEIGERTNIGCGSITVNYDGINKFKTSIGDDVFIGCNTNLVAPVTLGNRAFVAAGSTITDNVPNDSLALGRAKQTTKEGYLKK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should be able to enhance the efficiency of lipid A biosynthetic process, peptidoglycan biosynthetic process.2. The designed protein have MobA-like NTP transferase domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "PHPGLPTDVQAQLMVLAARADGRSRIVETVFPQRFAHVEQLRRFGIVIGRRDSHAWIDGPARLRPADAEATDLRAGAALVIAALCAPGRSLVRGIEHLQRGYEQLPEKL",
    "response#2": "MAAGHGTRMRSSLPKVLHRVCGRTMIEWVVGAARSAGAGRVVCVARPGSGLDRALPEGVELAEQRAGEGTGAAVLAARDQIEAGSSGGGGAVVILSGDHPLVTGELIEGLLAAHARAGAAATLLTTDRIDPAGYGRLTGPGGRPLGFTEQGVTVVDPASTWLDAGVTLARDVVLEPGVQLHGATSVGEGSRIGPDTTLTDVTVGEHAAVVRTHGSESVIGDGASVGPFAYLRPGTRLGESGKIGTFVETKQAEIGRGTKVPHLTYVGNATIGEYSNIGASSVFVNYDGVSKHHTTIGSYVRTGSDTMFVAPVEVGDGAYSGAGTVIRRDVPPGALAVSGSPPHPGLPTDVQAQLMVLAARADGRSRIVETVFPQRFAHVEQLRRFGIVIGRRDSHAWIDGPARLRPADAEATDLRAGAALVIAALCAPGRSLVRGIEHLQRGYEQLPEKL",
    "response#3": "MAAGHGTRMRSSLPKVLHRVCGRTMIEWVVGAARSAGAGRVVCVARPGSGLDRALPEGVELAEQRAGEGTGAAVLAARDQIEAGSSGGGGAVVILSGDHPLVTGELIEGLLAAHARAGAAATLLTTDRIDPAGYGALRAGGDDLMERIVEEASFDRLPRAEEV"
  },
  {
    "reference": "MGLSYGLFVCLLLWGGTELCYPQPIQQAGTHHPHPMPSKLPVVVECLEAQLVVTVSKDLFGTGKLIQSADLTLGPKNCEPLVSMDTEDVVRFEVALHECGNSVQVTEDALVYSTFLLHNPRPVGNLSILRTNRAEVPIECRYPRQGNVSSQAILPTWVPFQTTMFSEEKLVFSLLLMEEDWGAEKRSPTFQLGEIAHLQAEVHTGSHVPLRLFVDHCVATLTPDRNASPYHTIVDFHGCLVDGLSDASSAFKAPRPGPETLQFMVAVFHFANDSRNMVYITCHLKVTLADHVPDQLNKACSFSKSSNSWSPVEGPADICQCCGNGSCGTPGHSGTQSRTVRQWHKSASRNRRHVTEEADVTVGPLIFLGKASDRAEEGSTSSPASVMLGLGLAAVGSLTLATVGLGLARRRRAASHPVLCPVSAPQ",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein can be targeted to extracellular matrix and perform acrosin binding, receptor ligand activity.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. For general function, the protein need meet that The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4; ZP3 is essential for sperm binding and zona matrix formation. The designed protein sequence is ",
    "response#1": "ECQEAQLVVIVSKDLFGTGKLIRPADLSLGPAKCEPLVSQDTDAVVRFEVGLHECGSSLQVTDDALVYSTFLRHDPRPAGNLSILRTNRAEVPIECHYPRQGNVSSWAILPTWVPFRTTVFSEEKLVFSLRLMEENWSAEKMTPTFQLGDRAHLQAQVHTGSHVPLRLFVDHCVATLTPDWNTSPSHTIVDFHGCLVDGLTEASSAFKAPRPGPETLQFTVDVFHFANDSRNTIYITCHLKVTPADRVPDQLNKACSFR",
    "response#2": "CGPSSFHFSINLLSQDTATPPALVVWDRRGRLHKLQNDSGCGTWVHKGPGSSMGVEASYRGCYVTEWDSHYLMPIGLEEADAGGHRTVTETKLFKCR",
    "response#3": "RCTQDGHFSIAVSRNVTSPPLLWDSVHLAFRNDSECKPVMETHTFVLFRFPFSSCGTAKRVTGNQAVYENELVAARDVRTWSHGSITRDSIFRLRVSCIYSVSSSALPVNIQVFTLPPPLPETHPGPLTLELQIAKDERYGSYYNASDYPVVKLLREPIYVEVSIRHRTDPSLGLHLHQCWATPGMSPLLQPQWPMLVNGCPYTGDNYQTKLIPVQKASNLLFPSHYQRFSVSTFSFVDSVAKQALKGPVYLHCTASVCKPAGAPICVTTCPAV"
  },
  {
    "reference": "MTRLILATRNAGKIVELRAILAEAGLPHDLVGAETYPEIPDVKETGVTFAENALLKAHALAQATGHPAVADDSGLCVDVLGGAPGIFSARWSGTHGNDQANLDLLLAQLSDIAAPHRTAHFACAAALALPDGTERVVEGQLRGTLRPAPSGTNGFGYDPILQPDGETRTCAELSPAEKNEISHRGKAFRALVPVVRELLG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The Proton acceptor of the protein must be engineered to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+).2. The protein should have a high affinity for Mg(2+), substrate.3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.4. The designed protein must possess nucleotide binding, ribonucleoside triphosphate phosphatase activity, metal ion binding, dITP diphosphatase activity to support nucleotide metabolic process under certain conditions.5. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP The designed protein sequence is ",
    "response#1": "MKIIFATGNAGKVKELRAILGETGLEIVAQKALGCTDVEETGLSFVENAILKARHASQATGLPALADDSGLEVDALGGAPGVYSARYAGEDATDADNNAKLLKALQGQPRGQRRARFQCVLVYMQHPQEPTPIICQGQWEGRILEEPRGANGFGYDPVFYLPEYHCTSAQLPAALKNRISHRGQALQQLLAQLKT",
    "response#2": "MTITTPKAVLVAAGQGLRAGGAVPKQYRPIGGRSVLARAVDALAAHPEIDLVQPVIHPDDPDLYDAALSGLRRLDPPREGGSTRQESVRLGLEALNAAGITHVAIHDGARPYVTPALISRAIDAARSTGAAIPVLPVVDSLKRSADGRITGTVDRSGLFRAQTPQGFHFDSILAAHRAAAGEGRDDATIAEAAGMDVALVEGDEANFKITTPEDFARAERLLSASPV",
    "response#3": "MPIFEFVCPNCGYNVEKRFGGKSAPEKCPDCGAKQMESPALKI"
  },
  {
    "reference": "MQSSRGARAADVPVRLVVDIGNTSTTLAIFTGDEEPSVESVPSALFADSSTMREVFGNMARKHGEPQAIAICSVVPSATAVGSALLESLFSVPVLTICCKLRFPFRLDYATPHTFGADRLALCAWSRHLFSEKPVIAVDIGTAITFDVLDTVGNYRGGLIMPGIDMMAGALHSRTAQLPQVRIDRPESLLGRSTTECIKSGVFWGVVKQIGGLVDAIRGDLVRDFGESTVEVIVTGGNSRIIVPEIGPVSVIDELAVLRGSDLLLRMNMP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis.2. The protein design should incorporate a binding pocket that specifically recognizes NH4(+), K(+) to enhance enzymatic efficiency.3. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for (R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP +   H(+).4. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate, ATP, K(+) binding.5. A protein should be able to manipulate the activity of enzymes in the coenzyme A biosynthesis, Cofactor biosynthesis, CoA from (R)- pantothenate: step 1/5 pathway, either by activating or inhibiting their function.6. The designed protein should have metal ion binding, pantothenate kinase activity, ATP binding within cytoplasm to regulate coenzyme A biosynthetic process, phosphorylation. The designed protein sequence is ",
    "response#1": "MQGLNMYINEAXQXLKXPXLAKXIXDXVTXDXIXVXEXEXAXPXFXIAXFSXXAXGXKXGYIIXDXLSAXHXKXPXIGXLLNXSXINIQFIKXAXSRXLWXV",
    "response#2": "MSKKPQTMAQLEAQGLVWLGRNAADADWDALAAQDGHPLAWFRQEFLATHQSVQDEGVVWLMARADGQVVGGAALLRRGLPLPYARSWVTWPAWRNFFAARGGPVLIPDMKTEGAIVRALFDALAASRPGAAAVLTDIPAGDPAVAAVSGAGFHVERHYFAWTRPMADLTGDEEFLASLTAKHRSQIRRAEKKLDALGITLRHAGSGAGELDAALPAFMELYQATLDKWGGLPFTPQFFRDLAASMADRTRLLLAWHEGQPVAGAITLVSGDTLSGKLGASLPDYARYSPGKLLTFEMMAWGCEQGYRRYDFGGSDPETKLEAGLTRFKRHWGTEVVEYVGTWQAPLDPQNPRYEKLALAWEGVKQRLPLSLVNPLGRVVRKYIKR",
    "response#3": "MSKAYFPSDFEQPIADLEAKIAAGEPVVVTDDESRENEGDIIFAAEKATPELIAFMVRYTSGYICAPMSGEDCDRLQLPPMVSENQDYHGTAYTVSVDARDGVSTGISAVDRARTLQVLADPATKPSDFVRPGHVFPLRAKKGGVLVRAGHTEAAVDLARLAGLYPAGVLCEIMSEDGTMAREPELVKFADEHDLKIISIADLIEYRRRTEKLVEC"
  },
  {
    "reference": "MTRGLTIALDGMGGDHGPETVIGGADIASVRHPDIRFLIYGDETKIRPFLEKHPRVLSVSEIIHTDVSVSMEDKPSQALRRGRKTSSMWLAIDAVKAGEAQAAVSAGNTGALMAMAKVILRMMPNVERPALAALWPTARGESVMLDLGATVGADGYQLVQFAAMGEAFARVVFNIEQPTVGLLNIGEEEVKGTEGVKLAAQMLREADLPIRFHGFVEGDDIAKGTVDVVVTDGYTGNIALKTAEGMVRLVVDYLRAAMRSSLLSRLGYLLAYGAFRALAKKLDPRASNGAVFLGLNGLVVKSHGGTDAIGFAAAIDVAVDVASADLLSKIVTDLDRLSGLTTSMAGRKQNSDIAESEAVLS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA.2. The protein should be optimized for activity in phospholipid metabolism, Lipid metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "IVIAGISGRLPESNNLEEFWQNLFNGVDMVTEDDRRWKPGLYGLPRRNGKLKEIDRFDAAFFGVHPKQAHTMDPQLRLMLEISYEAIVDGGINPVSMRGSKTGVYIGVSGSEAGEAFSKDPEELLGYSMTGCQRAMFANRLSYFFDFNGPSTAIDTACSSSLLALENAFNAIRHGKCDAALIGGVNLLLKPNTSVQFMKLGMLSPEGTCKSFDASGNGYCRSEAAVAVLLTKKSMAKRIYATVLNAGNNTDGYKEQGVTFPSGEMQQRLVRSLYQEANISPEQVEYIEAHGTGTKVGDPQEVNGIVSVFCQSQRDPLLIGSTKSNMGHPEPASGLAALAKVVLSLEHGVWAPNVHFNEPNPDIPALTDGRLCVVSKPTPVRGGIVGINSFGFGGSNVHVILSPHVTDRKSK",
    "response#2": "IVIAGISGRLPESNNLEEFWQNLFNGVDMVTEDDRRWKPGLYGLPRRNGKLKEIDRFDAAFFGVHPKQAHTMDPQLRLMLEISYEAIVDGGINPVSMRGSKTGVYIGVSGSEAGEAFSKDPEELLGYSMTGCQRAMFANRLSYFFDFNGPSTAIDTACSSSLLALENAFNAIRHGKCDAALIGGVNLLLKPNTSVQFMKLGMLSPEGTCKSFDASGNGYCRSEAAVAVLLTKKSMAKRIYATVLNAGNNTDGYKEQGVTFPSGEMQQRLVRSLYQEANISPEQVEYIEAHGTGTKVGDPQEVNGIVSVFCQSQRDPLLIGSTKSNMGHPEPASGLAALAKVVLSLEHGVWAPNVHFNEPNPDIPALTDGRLCVVSKPTPVRGGIVGINSFGFGGSNVHVILSPHVH",
    "response#3": "IVIAGISGRLPESNNLEEFWQNLFNGVDMVTEDDRRWKPGLYGLPRRNGKLKEIDRFDAAFFGVHPKQAHTMDPQLRLMLEISYEAIVDGGINPVSMRGSKTGVYIGVSGSEAGEAFSKDPEELLGYSMTGCQRAMFANRLSYFFDFNGPSTAIDTACSSSLLALENAFNAIRHGKCDAALIGGVNLLLKPNTSVQFMKLGMLSPEGTCKSFDASGNGYCRSEAAVAVLLTKKSMAKRIYATVLNAGNNTDGYKEQGVTFPSGEMQQRLVRSLYQEANISPEQVEYIEAHGTGTKVGDPQEVNGIVSVFCQSQRDPLLIGSTKSNMGHPEPASGLAALAKVVLSLEHGVWAPNVHFNEPNPDIPALTDGRLCVVSKPTPVRGGIVGINSFGFGGSNVHVILSPHVSNQKGFLDSPPRVYILVAPHRTWWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINVLKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGG"
  },
  {
    "reference": "MAELACFCYPHLENDSYKFIPFNNLAIKAMLTAKVDKKDMDKFYDSIIYGIAPPPQFKKRYNTNDNSRGMNFETIMFTKVAMLICEALNSLKVTQANVSNVLSRVVSIRHLENLVIRKENPQDILFHSKDLLLKSTLIAIGQSKEIETTITAEGGEIVFQNAAFTMWKLTYLEHQLMPILDQNFIEYKVTLNEDKPISDVHVKELVAELRWQYNKFAVITHGKGHYRIVKYSSVANHADRVYATFKSNVKTGVNNDFNLLDQRIIWQNWYAFTSSMKQGNTLDVCKRLLFQKMKPEKNPFKGLSTDRKMDEVSQVGV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activities; Participates in replication and packaging of the viral genome; The RTPase activity plays a role in the removal of the gamma-phosphate from the rotavirus RNA minus strands of dsRNA genome segments.; The unwinding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures2. helix-strand-strand-helix-helix-strand-turn-strand-helix-strand-helix-helix-helix-helix-helix is the desired arrangement for the engineered protein.3. The designed protein should be optimized for hydrolase activity, acting on acid anhydrides, metal ion binding, RNA binding activity in order to improve viral genome replication.4. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.5. The ATP binding site should be stable and able to withstand changes in environmental conditions.6. The For NTPase and RTPase activities active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "DARLSSPIVLDQCDLVTRACGLYSEYSLNPKLKTCRLPKHIYRLKYDTIVLRFISDVPVATIPIDYIAPMLINVLADSKNVPLEPPCLSFLDEIVNYTVQDAAFLNYYMNQIKTQEGVITDQLKQNIRRVIHKNRYLSALFFWHDLAILTRRGRMNRGNVRSTWFVTNEVVDILGYGDYIFWKIPIALLPMNTANVPHASTDWYQPNIFKEAIQGHTHIISVSTAEVLIMCKDLVTSRFNTLLIAELARLEDPVSADYPLVDNIQSLYNAGDYLLSILGSEGYKIIKYLEPLCLAKIQLCSQYTERKGRFLTQMHLAVIQTLRELLLNRGLKKSQLSKIREFHQLLLRLRSTPQQLCELFSIQKHWGHPVLHSEKAIQKVKNHATVLKALRPIIIFETYCVFKYSVAKHFFDSQGTWYSVISDRCLTPGLNSYIRRNQFPPLPMIKDLLWEFYHLDHPPLFSTKIISDLSIFIKDRATAVEQTCWDAVFEPNVLGYSPPYRFNTKRVPEQFLEQEDFSIESVLQYAQELRYLLPQNRNFSFSLKEKELNVGRTFGKLPYLTRNVQTLCEALLADGLAKAFPSNMMVVTEREQKESLLHQASWHHTSDDFGEHATVRGSSFVTDLEKYNLAFRYEFTAPFIKYCNQCYGVRNVFDWMHFLIPQCYMHVSDYYNPPHNVTLENREYPPEGPSAYRGHLGGIEGLQQKLWTSISCAQISLVEIKTGFKLRSAVMGDNQCITVLSVFPLESSPNEQ",
    "response#2": "LKKQSKDWLASFALKTENIEREVLMSVIHGAGGSGKSHAIQTWMRSLNRRDRHVTIILPTTDLRNDWTNKVPNLEQANFKTFEKALCQPCGKIIVFDDYSKLPQGYIEAFLAINQNVILAILTGDSKQSFHHESNEDAYTATLEPSIITYQPFCRYYLNITHRNKPDLANKLGVYSCSSGTTSFTMSSQALKGMPILSPSIMKKTALGEMGQKSMTYAGCQGLTTKAXHLLCPTDCFRKHPEATYGRCGSGPWLTPRCLVHYEYRLWHYPCTVNYTLHKVRMFIGGVEHRFEAACNWTRGEPCELDDRDRIEMSPLLFSTTQLAILPCSFTTMPALSTGLIHLHQNIVDVQYLYGVTTHIVSWAVKWEYVVLLPDTSSNRFYTLESKQWMENSKGWWWKLPDALKDMGIFGENMFYHFLGRAGYTIHVQCNASKFHQGTLMVVAIPEHQLAYIGTGNVTVGYKHTHPGESGRIIANNADNNTRQPSDDNWLNCDGTLMGNLPIFPHQFINLRTNNSATLILPYMNCVSMDSMLRHNNWSLLIIPVCPLQVDAQATRFVPITISISPMSAEFSGARNAVSGGTIARATHGFTGLFAPGAQQNIRLIHTDGSWHINSTALNCNDSLNTGFLAGLFYYHXFNSSGCPARLASCRRLDDFSQGWGPLTYNSSSTAGINRPYCWHYAPRQCEVVPAKNVCGPVYCFTPSPVVVGTTDSRGVPTYNWGENETDVIILNSTRPPNGAWFGCTWMNGTGFTKACGAPPCAVQSVNGTWPCPSDCFRKHPETTYAKCGSGPWLTPRCLVDYPYRLWHYPCTVNYTIFKVRMYVGGIEHRLNAACNWTRGEPCDLEHRDRAELSPLLLSTTQWQVLPCSFTTMPALSTGLIHLHQNIVDVQYLYGVGSAVVSWALKWEYIVLAFLLLAD",
    "response#3": "LKKQSKDWLASFALKTENIEREVLMSVIHGAGGSGKSHAIQTWMRSLNRRDRHVTIILPTTDLRNDWTNKVPNLEQANFKTFEKALCQPCGKIIVFDDYSKLPQGYIEAFLAINQNVILAILTGDSKQSFHHESNEDAYTATLEPSIITYQPFCRYYLNITHRNKPDLANKLGVYSCSSGTTSFTMSSQALKGMPILSPSIMKKTALGEMGQKSMTYAGCQGLTTKALTKEIETTATAEGGEIVFQNVAFTMWKLTYLDHKLMPILDQNFIEYKITMNEDKPISDVHVKELIAELRWQYNRFAVITHGKGHYRVVKYSSVANHADRVFATYKNNAKSGNVIDFNLLDQRIIWQNWYAFTSSMKQGFTLDVCKKLLFQKMKQERNPFKGLSTDRKMDEVSRIRGSSFVTDLEKYNLAFRYEFTAPFIKYCNQCYGVRNVFDWMHFLIPQCYMHVSDYYNPPHNVTLENREYPPEGPSAYRGHLGGIEGLQQKLWTSISCAQISLVEIKTGFKLRSAVMGDNQCITVLSVFPLESSPNEQERCAEQLQIFELLRKRKQIVVTGSTGIGKTSQLPKVIMWYNYLFGGWDNLDRVRFDYISRPIVLSLPRVALVKSNGINFLQSLGFSDFEGSPVELRYGGKTEHTTRQHDGIVLSTNKLTSYSLSNYNIIIVDEIHEHDRIADIIISVLRKNIDTIHSLVLMSATLEDDL"
  },
  {
    "reference": "EDICSLPPEVGPCRAGFLKFAYYSELNKCKLFTYGGCQGNENNFETLQACXQA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The presence of BPTI/Kunitz inhibitor domains is critical for the interaction of the designed protein with its intended targets.2. For general function, the protein need meet that Inhibits trypsin.3. A protein with serine-type endopeptidase inhibitor activity is needed to enhance negative regulation of peptidase activity in a specific context. The designed protein sequence is ",
    "response#1": "IHLEPVAGTLPCGHTETITAHYTLNRQAMGELSELSFHYLIMAKGVIVRSGTHTLPVESGDMKGSFALSFPVESDVAPIARMFIFAILPDGEVVGDSEKFEIENCLANKVDLSFSPAQSPPASHAHLQVAAAPQSLCALRAVDQSVLLG",
    "response#2": "LKFVKVDSHFRQGIPFFAQVLLVDGKGVPIPNKLFFISVNDANYYSNATTNEQGLAQFSINTTSISVNKLFVRVFTVHPNLCFHYSWVAEDHC",
    "response#3": "LKFVKVDSHFRQGIPFFAQVLLVDGKGVPIPNKLFFISVNDANYYSNATTNEQGLAQFSINTTSISVNKLFVRVFTVHPNLCFHYSWVAEDHC"
  },
  {
    "reference": "MPETGVRNGAFGADKFGLKNLEAVYWNFGAPQLYEHALRKGEAMVNADGALCADTGVFTGRSPKDKFTVRDATTDQSVWWAGNQSITPEQFAALYADFLDHAEGMTLFAQDLYGGADPNFRIPTRVFTELAWHSLFIRTLLRRPDTSELAGFVPELTVIDLPSFRADPERHGVRSENVVAIDFVRKIILIGGSHYAGEMKKSIFTTLNYYLPDNGVLPMHCSANVGPEGDSTIFFGLSGTGKTTLSADPKRTLIGDDEHGWSKEGIFNFEGGCYAKCIKLSQEAEPEIYAASKRFGAVLENVVHDESTRVPDFDDGSKTENTRSAYPLEFIPNASLTGRAGQPKNLVMLAADAFGVLPPIARLTPAQAMYHFLSGYTAKVAGTERGLGNEPQPEFSTCFGSPFLPRDPSVYGNMLRELIARHNVDCWLVNTGWTGGKYGTGRRMPIKVTRALLGAALDGSLREVQFRTDRYFGFAVPTSVPGIEPHILDPIKTWADKAEFDKTACALVGMFQNNFTRFEGDVDAEVRAAAPEVRAAAE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a binding site that is specific to the target ATP ligand.2. The protein must exhibit the following characteristics: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA..3. The protein must bind to Mn(2+) in order to perform its enzymatic function.4. The protein can be targeted to cytoplasm and perform ATP binding. The designed protein sequence is ",
    "response#1": "PIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENGVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDSPISSLHEAHEFSNTFGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPATHLDPQLRSRLTSDSVKLAKQVGYENAGTVEFLVDKHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVSEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPL",
    "response#2": "MFSKILVANRGEIAVRVMRACRELGIKTVGVYSSADKRAFHRVYADECYYIGKADPRDSYLNIDRIIEVAKKSGAEAIHPGYGFLAENAEFAERCEEEGIVFIGPSPEVIRIAGSKVRSRESMQRAGVPVIPGSPKIDTVDEAKEWAEKIGYPVAVKASGGGGGIGIVVVNSQEELEEAFRKSKKLGESYFKDSTVYLEKYLARPRHIEVQILADQHGNVIHLGERECSIQRRHQKLIEEAPSPALNEEMREELGKLAVKGAREIGYTNAGTFEFLYENGNFYFLEINSRLQVEHTITEVVTGIDIVKYQIRIAYGEELRHGQEDVAIRGHAIECRINAEDPVNFYPRSGRILHYRSPGGIGIRVDSGIHMGYRIPEEYDSMISKLIAYGETREEAIARMKRALYEYIIEGVETNIPFHFAVLNDEEFVRGNIHTKFVEERNG",
    "response#3": "MFSKILVANRGEIAVRVMRACRELGIKTVGVYSSADKRAFHRVYADECYYIGKADPRDSYLNIDRIIEVAKKSGAEAIHPGYGFLAENAEFAERCEEEGIVFIGPSPEVIRIAGSKVRSRESMQRAGVPVIPGSPKIDTVDEAKEWAEKIGYPVAVKASGGGGGIGIVVVNSQEELEEAFRKSKKLGESYFKDSTVYLEKYLARPRHIEVQILADQHGNVIHLGERECSIQRRHQKLIEEAPSPALNEEMREELGKLAVKGAREIGYTNAGTFEFLYENGNFYFLEINSRLQVEHTITEVVTGIDIVKYQIRIAYGEELRHGQEDVAIRGHAIECRINAEDPVNFYPRSGRILHYRSPGGIGIRVDSGIHMGYRIPEEYDSMISKLIAYGETREEAIARMKRALYEYIIEGVETNIPFHFAVLNDEEFVRGNIHTKFVEERNL"
  },
  {
    "reference": "MKADLATAKGSSPAFSAPRTYRARLLSRCLNKCFNTVLVSGGAAESRFRGGATVGKAGAHDARGVGDLVDFLQQEATPYEVNVPPYLILPPLKSNEATTHRRAGDSGGQGPAAAGGRGQARHGRRQAAASAATTVSAAPQTGATKPAATAKTTPQRPRGSDADAGSRAQSQYGFGDPPGGGALKGAVDAASDAAPDVAAASPAPAGISDQLSTPACPPEREPQAGKPRASGRAPAAPGVGPQDVGGGSGACAPAPDESHMGLTHRDQGHDERISQTAGEAWKAGAVAAPPAAPTPSPPGLAAAPTRLASSALGTHSSDGDMRRAVPGRDTPSLSAVAGPVTLSGSSSSSSGRNSSSNSNTSTSSTSNGVTITSNVGVNGASPQERLMAARRAVVTMQWNTHLGRRGRSFAPLPTGGMSIATSAASSSTSSASSSSSMNDGSNAKKTSDAAVSLPVGQQPAAEQPHVPTAPGGPSQTGASAVAAQAPSSAMPTAAMAATMGSATIGSAATLPTAAVVSSAAAEGTQPSGLLLAGGRPALLGRTIQGRIARLQAAREALRAARHARVGAAMQPPPVQARPVQGQSGQVPQVGQGPVQSQPGRRQEPAASATKLHVADGLPARPVQPAVSATDLQTDTATAASAPFPVSDASLGSTEPLAASAPTTSLASASGPAIGTSSSNAHSSAAASEAAAGTVRAPTDTAAPAASPAATAPALASTPFATPAAAPLPEPPEVVAARDLLGRLSRYQPAGRDDGPAVLAPHARYSPAA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Required for trans-splicing of exons 1 and 2 of the chloroplast encoded psaA mRNA (a group II intron); May be required for stability of the chloroplast RNA-protein complex in which it is found..2. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "KKLDTLFPTIPMIGSLAKVKFCNTLGHPISKSSWADLSDSDIIGRFVRIGGNLSHYYSGSSKKKSLYRIKYILRLSCVKTLARKHKSTVRTFLKRLGPKLLDEFFTEEEQVFALG",
    "response#2": "ELCPIIPFLLLVNSLARGGFCTNLGRPVSKLSWTTLTDDDILKKFDQIWRSVYYYYSGSINNHGLFRLRYIFRFSCAKTLACKHKSTTRIVWKRFSLNSFLRSFLKKPELR",
    "response#3": "ELCPIIPFLLLVNSLARGGFCTNLGRPVSKLSWTTLTDDDILKKFDQIWRSVYYYYSGSINNHGLFRLRYIFRFSCAKTLACKHKSTTRIVWKRFSLNSFLRSFLKKPELX"
  },
  {
    "reference": "MDRFQIGDEQLLRFYQLKTINPTHSWAQDSSKLNNEEATSNELGVETSFDILKDFKYGNQISIDKESRAYLNDESLSYIRDPLNGQEMSKELQHLPNDSMRLNYLVNSKQFNVKAFLRDMHKQDSFNDLNNSLDRLDSDIQDQSIHLKQLVGKNFTKYVKIKNKLDQIYKEFDEKTNEKNQCDSPKENQINVESLNKKVDEVIRTTTFKLKPLMDNYQKILNYQATKKFIELNKFYFNLPKSLKRCLTNNDFNEFIIEYSKGLTLRRRFNQSSDASQSLVIKRIWTQIENLLVTYKDLIWNSLINSNFNIDQPQETILSLFSKLLNLENFINNNQRESESGNKNTTSSSNENPILRWMSIKMNGFQNELNELSGHMISKIIHSQRLILQNNTNQDKSQGCVELSYYLKINQLFQIISDTGKDSEGLKSTVEPNKVNTISGTSYLNLNCQPSSQGLTDSPTIIEMWLLILKYINDLWKICDQFIEFWEHIEKFLDGTYQNSIINEKRKENILIGDSNIIESYQKSLILKEEQINEVRLKGEEFITSVSQNLISFFTSSQSSLPSSLKDSTGDITRSNKDSGSPLDYGFIPPNCNGLSCLRYLPKIVEPILKFSTELAQLNITTNGITICRNTLSTIINRCVGAISSTKLRDISNFYQLENWQVYETVTFSSKSQDSSKNLTFEYGVTQFPEIVTSFQEVSIKTTRDLLFAYEKLPIINGISVVSYPSKQLLTGIEIQQIISMEAVLEAILKNAAKDKDNPRNSHTILTL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Incorporate a signal peptide in the protein design.2. The protein should be optimized for stability and solubility when expressed in exocyst where it will be involved in endoplasmic reticulum inheritance, vesicle tethering involved in exocytosis, protein transport, exocytosis, Golgi to plasma membrane transport. The designed protein sequence is ",
    "response#1": "LRNLCVELIDAFGGALILKHSVFFEEAFEKDVYAPMIVETQEEYDHYIAPYWNFPSEPFPRTMSFSKMCPLCCTTLSKFVRHFFMFLNDSVILATEVNEKAPRFYRRFILRSLVDRLKSLYPKLALSQMSQLVKNFYAFEDPLLQIEKSLVLNKPIHQIGAEASSSNNVKSTELLEGLANARKSALHEIFVKINLKIDDFLGLAEYDWTTTQVRKDVSGYLQEMVTYLQTMYLESLAGLPKHDKSYVYLETLDHLCTAMVDLLSDPSIRKVTTAAAEGFKLDVEYLESFAAQVPDQSIVNADSFIELRQCANLLLGDNMEEYLDTDKFMRDFNRLQPAVAIKFLENA",
    "response#2": "LRNLCVELIDAFGGALILKHSVFFEEAFEKDVYAPMIVETQEEYDHYIAPYWNFPSEPFPRTMSFSKMCPLCCTTLSKFVRHFFMFLNDSVILATEVNEKAPRFYRRFILRSLVDRLKSLYPKLALSQMSQLVKNFYAFEDPLLQIEKSLVLNKPIHQIGAEASSSNNVKSTELLEGLANARKSALHEIFVKINLKIDDFLGLAEYDWTTTQVRKDVSGYLQEMVTYLQTMYLESLAGLPKHDKSYVYLETLDHLCTAMVDLLSDPSIRKVTTAAAEGFKLDVEYLESFAAQVPDQSIVNADSFIELRQCANLLLGDNMEEYLDTDKFMRDFNRLQPAVAIKFLEH",
    "response#3": "TEPGEETGVFTIFGSNSQHLSWVIIQVNLSSAFNRTCVDSDYYSWNPTDDPNIDCLLGKKMIYQRRKSLSLCYNGRNYDRAINVQNCPCKISDYECDYGYTRNKTTQLCELRSFEWSSFVCAFGYDLSYNRSKGYKRIPSDTCEGGMQNVFDPVLTNC"
  },
  {
    "reference": "MTQSVGLVGWRGMVGSVLMQRMRDENDFSLFQPVFFSTSNAGGKAPAWADGAGPLQNAYDIETLKKLPIIVTAQGGDYTTEVYPKLRAAGWDGVWIDAASTLRMKDDAIIVLDPVNRAVIDAALQRGVKNFVGGNCTVSCMLMGLAGLFNNDLVDWMTSMTYQAASGGGAQHMRELLTQFGIINQSVKSLLDDPASAILEIDRGVLQTQKDADLPRDHFGVPLAGSLIPWIDKDLGNGVSREEWKAEAETNKILGRGEAFGTEPTRIDGLCVRIGAMRCHSQALTLHLKRDVPLDEITDIIREGSKWAKVIPNEREATMQGLTPVAVTGTLDIPVGRLRKMSMGPQYLSAFTVGDQLLWGAAEPLRRMLRITLGEL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have a binding site that is specific to the target substrate, phosphate, NADP(+) ligand.2. The designed protein should be optimized for protein dimerization activity, aspartate-semialdehyde dehydrogenase activity, NAD binding, NADP binding activity in order to improve threonine biosynthetic process, isoleucine biosynthetic process, diaminopimelate biosynthetic process, lysine biosynthetic process via diaminopimelate.3. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH are properly positioned for optimal catalysis.4. A protein should be able to manipulate the activity of enzymes in the Amino-acid biosynthesis pathway, either by activating or inhibiting their function.5. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.6. The designed protein should contain one or more Semialdehyde dehydrogenase NAD-binding domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MRISVVGATGAVGRTMLSILEERDFDVSELRVMASARSAGRSLSTKWGDVTIEDLSSADPSDIDIALFSAGGARSREYAPRFVESGAVVVDNSSAFRMEEGVPLVVAGVNDAALQGPPKGIIANPNCTTLSMLVALAPLHKKYQINRVVVSTYQSVTGSGVKAVDQLEGERNGQPVEKAYPHPIDKNVLPHIDIFMDNGYTREEMKMVNETKKIMGDDNLKVTATAVRIPVIGGHSESVNIEFEQEYDLQEVRDILANTPGIVLQDDNANNVYPMPLNAHGKDEVFVGRLRRDETQPKTLNMWIVADNLRKGAALNTYEIAEALVRVAIMGATGAVGAELLQLLEERSFPVKDLVLLASPRSVGKELMFRGQPIKVQAISERNLADIDLVLASAGGSVSREWAPRAVALGAVVVDNSSAFRMDPSVPLVVPEVNPETVLESPIHKNFGIKSMVISTYQSITGTGQKAMTQLENELKGFSTEMAYPHQIHKNAIPHCDDFQDNGYTKEEMKLVNETHKILNDHDIGITATAVRIPVIGGHSESVNLTLKKRTSIDEIKNILKNSEGLVVQDDPKNNYYPMPITAEGNDSVYVGRIRKDFSKSNSLNLWIVSDNLRTNVNWPLKPIHDAAIIDRLVISTYQAVSGTGDEAIQELHEQTRHIFLQRDIVCQVYPHQIAFNCLPHIDVFLENGCSRQEMRVVHETRKILEDDGIRITATAVQVPVFYGHSASVNIETDRKITADDVRELLSNAPGVTVIDDPCHHLYPLATDAAGEDAVFVGRIREDDSIETGINLWIVSDNVR",
    "response#2": "MKSTLAVVGATGAVGRIIVELLVSRRFPAKRIRLLASARSAGRPMEVAGTRFEVEELRPESFAGVDVAIGSTPDEVAAEFVPWAVERGTVVVDESAFYRMNPGVPLVIPEVNPGAIAGALAAGGRNWIGGNCTVSCMLMGVGVKPLHDAARVRRVVVSTYQAVSGAGLAASQELRQATAGWLAGGAEPRQIFPHPIAANLIPQIGSPRPNGFTSEEMKMVLETRKILGDESIQVSATCIRVPVANGHSESILIETETKLSADKARHLLAGFPGVVVEDDLGAKHYPQPRTASGRDEVFVGRIREDISCPNGIALWCVSDNLRWGAAEPLRRMLRLKPIHDAAIIDRLVISTYQAVSGTGDEAIQELHEQTRHIFLQRDIVCQVYPHQIAFNCLPHIDVFLENGCSRQEMRVVHETRKILEDDGIRITATAVQVPVFYGHSASVNIETDRKITADDVRELLSNAPGVTVIDDPCHHLYPLATDAAGEDAVFVGRIREDDSIETGINLWIVSDNVRLQPVEHRWGIRQVVASTYQAASGQGHHGIEELLEGTELALRDPDAELPAERFKPPLAFNVVPSIDRLLDTGFTLEEQKMLQESRKILDRPHLDLTATCVRVPVVNSHSEAVWVEAGAPVDRKELVGLLAALPEVTVHDAENPAAFPTPLRAGHPDHVHVGRIRVSPDNPRGFWLWLVSDNLH",
    "response#3": "MTNVAVVGATGQVGGVIRRLLDERDFPVDRLRFFSSPRSAGTTLEWRDRTITVEDITATATDDLRGIDLALFSAGGAASREHAPRFAEAGAIVVDNSSAWRRDPDVPLIVSEVNPEALADEDKIIANPNCSTIQMVMALAPLHKKYKIKRIVVSTYQSISGTGVKAVKQLENEMAGVKGEMAYPYPIHKNAIPHCDVFEENGYTKEEMKLVRETQKILDDKSIAVTATAVRIPTSGGHSEAINVEFENDFNLTEVREILNATDGVTLQDNVDVNTYPMPLYANGKDDVFVGRLRRDGSLPNTLNMWVVSDNLRKGAATNTVQIAEYLIDNNLV"
  },
  {
    "reference": "MSDLVFFPSGSEQQDRRNLLDFDASGLAAWFADMGEKPFRARQVLRWMHRSGASDFDAMTDLAKSLRQKLRTRAEIRPPVLRSDHFSEDGTRKFLFDVGNGNAVETVFIPEDDRGTLCISTQAGCALECAFCATGKQGFNRNLTTAEIIGQLWLANRALGAGLGGERVISNVVLMGMGEPLANFENSVTALRLMLDDNAYGLSRRRVTVSTVGLVPAIDRLREECPVALAVSLHAPSDALRDQLLPINRKYPLAELMAACHRYLERAPRDFITFEYIMLDGINDSEAQARELLALTSELPCKFNLIPFNPFPGVPFRRSQPERLRRFAEVLMSAGVVTTTRKTRGEDINAACGQLAGQVGNRMRRLVSRTYPLQEVHP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs; m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity..2. The designed protein should have a high specificity for S-adenosyl-L-methionine, [4Fe-4S] cluster binding, with minimal off-target effects.3. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.4. The protein contains novel Radical SAM core domains that confer a unique function or activity.5. The designed protein should be optimized for 4 iron, 4 sulfur cluster binding, tRNA binding, rRNA binding activity in order to improve rRNA base methylation. The designed protein sequence is ",
    "response#1": "MEPLDLVGLSREDLGTALANVGVPQKQVRMRVSQLWHWLYMRGVNDFDRMSNISKEMRALLKANFTIGWPEIADEQISNDGTRKWLLRFPSRGAGRPVEVETVYIPEEGRGTLCVSSQVGCTLTCSFCHTGTQKLVRNLTSGEILSQLILARHRLGDFPDVEAAPGTMVPSEGRKVSNIVMMGMGEPLYNFENVKQALLIASDGEGLSISRRRITLSTSGVVPEIRRAGEEIGVMLAISLHAVRDDLRDELVPINKKYPLKDLLQACRDYPGLSNARRITFEYVMLKGVNDSLEDARDLVKLLKGIPAKINLIPFNPWPGSAYECSDWEQIERFADFVNHAGYASPIRTPRGRDILAACGQLKSESERLRKSSRRS",
    "response#2": "LAGMTRNQIRDCLIATGLVEEKKAKMRSGQIWRWVYQYGITDFAAMTDISKELRERLPEHYTLPALEVECVQVSKDGTRKYLFRLEDGNIIETVLMPAAPEGGCVRAWGEPAYRGKQIFDWIHRQGVLDPEQMSNLPKLLRERMKEGEPLDLVGLSREDLGTALANVGVPQKQVRMRVSQLWHWLYMRGVNDFDRMSNISKEMRALLKANFTIGWPEIADEQISNDGTRKWLLRFPSRGAGRPVEVETVYIPEEGRGTLCVSSQVGCTLTCSFCHTGTQKLVRNLTSGEILSQLILARHRLGDFPDVEAAPGTMVPSEGRKVSNIVMMGMGEPLYNFENVKQALLIASDGEGLSISRRRITLSTSGVVPEIRRAGEEIGVMLAISLHAVRDDLRDELVPINKKYPLKDLLQACRDYPGLSNARRITFEYVMLKGVNDSLEDARDLVKLLKGIPAKINLIPFNPWPGSAYECSDWEQIERFADFVNHAGYASPIRTPRGRDILAACGQLKSESERLRKSASLR",
    "response#3": "LAGMTRNQIRDCLIATGLVEEKKAKMRSGQIWRWVYQYGITDFAAMTDISKELRERLPEHLRIGRPQVEQESSSDGTIKWLIEVDAGNGIEAVLIPCVRAWGEPAYRGKQIFDWIHRQGVLDPEQMSNLPKLLRERMKEGINHPVIAEVQKSDDGTRKWLLSVGNGNAVETVFIPEDDRGALCVSSQVGCTLTCRFCHTGTQLLVRNLTAHEIVGQFMAARDAYGEWPSPTDESRQLSNIVLMGMGEPLYNYDNVAKAIGILLDNEGIAVSRRRITLSTSGVVPMIRRCGAELGVNLAVSLHAARDEIRDEIMPINRKYPLAELMAACREYPGASNARRITFEYVMLKGVNDSEADARALIKLVEGVPCKFNLIPFNPWPGSGFECPPIRHIERFANILFEAGYTAPIRMPRGREIEAACGQLRGRVDADRGTLCVSSQVGCTLNCTFCHTGTMKLVRNLTAGEIVGQVMLARDGLGEWPSQPEGRMLTNIVMMGMGEPLYNFENVRDALSVVMDGDGLALSKRRITLSTSGVVPMMARAGAEIGVNLAVSLHAVTKPVRDEIVPLNRKYGIEELLQACADYPGANNARRITFEYVMLKDKNDSDDDARELVRLLRHYKLPAKVNLIPFNPWPGANYECSTPERIRSFSNIVFEAGISAPVRTPRGRSRVAVEAACGQLRGRSVGRSGALCVSSQVGCTLNCTFCHTGTQALVRNLTAAEIVQQVLIAKDDLAEWPSRLEDRQITNVVFMGMGEPLYNLDHVADAIDVISDGEGIGIGRRRITVSTSGVVPKIEELGARTNTMLAISLHATNDALRDELVPLNKKYPIAELLDAIRAYPELSNSKRVTFEYVMLKGVNDSPGEAKALVRLLQGIPSKINLIPFNPWPGSPYECSDWATIERFADIVNKAGYASPI"
  },
  {
    "reference": "MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGKDFPVFLHPETHEEYALARTERKTAKGYAGFSFHADKDVTLEQDLMRRDLTINAMAQDAGGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQMVENGEADALVAERVWQELAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRADYHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPPDILPRHHGHDLAGVEPVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQTALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHLIAEQIDRMRLAQIAPLQKAFQAAQDKTEKH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The presence of HD domains is critical for the interaction of the designed protein with its intended targets.2. The desired protein should be able to perform a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3   diphosphate3. The protein requires Ni(2+) to activate a critical catalytic site.4. The protein design must be able to perform ATP binding, magnesium ion binding in order to contribute to tRNA 3'-terminal CCA addition.5. The designed protein should have a binding site that is specific to the target Mg(2+) ligand.6. The protein must exhibit the following characteristics: Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate; Also shows phosphatase, 2'-nucleotidase and 2',3'-cyclic phosphodiesterase activities. The designed protein sequence is ",
    "response#1": "MKTYIVGGAVRDELLGVAVKDRDYVVVGATPEAMVAQGYTPVGKDFPVFLHPKTHAEYALARTERKTAPGYKGFVFHTDASVTLEEDLIRRDLTINAMAQDEDGSIVDPFGGKRDLDAARHRILRHVSDAFAEDPVRILRLARFAARFHDFSIAPETLAMMWEMVTDGEVDGLVPERVWQELARGLMEDKPSRMFDALRACGALQHLLPEVLDTGIHTLLVAEQAAKLSSSAVVRFAAQVHDLGKGVTPPSEWPSHKMHCHTGLKLIKQLCERVRVPNEFRDLALMVCEQFGQPDYIANLPEYPQLTSLASGWAALIFLLRTQPLVMLKAWKQSNELITLVTQTVKLLQVMPNPTAWDLYQAGEAAVTTASEVQKLLTPDFDQQQLAEAYAALPIHSKKELALTGADLIKAGVRPGPAMGKALNQIEQRVVAGALPNELKKLL",
    "response#2": "MQIYMVGGAVRDRLLGRPVNDHDWVVVGATPDDMVARGYLPVGRDFPVFLHPETREEYALARTERKSGRGYRGFVVQTSPDVTLEEDLSRRDLTINAIAASAAWTGAEDLFDPYGGARDLQARLVRRISDAFARRNDAKYFKIFNKLINTYGAESYLGGLSGYACEILTIHYKGFTNLLKGILKWKPKVFIDIEKHYSNIKEAINELGKDKTASPLILIDPVDKTRNVAASLSYKNFALIIANSYLYLNNNDAKYFKIFNKLINTYGAESYLGGLSGYACEILTIHYKGFTNLLKGILKWKPKVFIDIEKHYSNIKEAINELGKDKTASPLILIDPVDKTRNVAASLSYKNFALIIANSYLYLNNKGTHLITLLFDVPEMVDDLLFPQLERSARGFEKALSREGFEVLGWNTGRYGAEKAFVMLELDRVERPRVKIHPGPEFFTERGRDFYRKNERVWLVGKRLYAEKRVKENIIDVVRELLEKNQVALGKNLRETVKGAEL",
    "response#3": "MQIYMVGGAVRDRLLGRPVNDHDWVVVGATPDDMVARGYLPVGRDFPVFLHPETREEYALARTERKSGRGYRGFVVQTSPDVTLEEDLSRRDLTINAIAASAAWTGAEDLFDPYGGARDLQSRIVRRRFALQGDREKAQELGLEVKPYFVGSLAKDTYLAGDHDVDLFLAFPLDTPLEEVRERGLELGKEIGKTLGEYEIAYAEHPYVRAKYRDVKVDLVPCYDVRDWKDVRTAVDRSILDFSIAPETLAMMWEMVTDGEVDGLVPERVWQELARGLMEDKPSRMFDALRACGALQHLLPEVDTMLEITFRVEETKEDIKNAKISRLTRKIVNYLEKNGIEVYAYTIDFDKNKSYLWVCCEKITIKTKHLGPPAWVNLDKFLEKHNKFFIREDGRLYTIINE"
  },
  {
    "reference": "MAAAAAAATTAACSSGSAGTDAAGASGLQQPPPQPQPQPAAAAPAQPPPEPPRKPRMDPRRRQAALSFLTNISLDGRLPPQDAEWGGGEEGGAAKPGAGGACGARTRFSLLAAAERGGCIALAAPGTPAAGLAAGSGPCLPQPSSLPPLIPGGHATVSGPGVARGFASPLGAGRASGEQWQPPRPAPLAACAQLQLLDGSGAAGQEELEEDDAFISVQVPAAAFLGSGTPGSGSGSRGRLNSFTQGILPIAFSRPTSQNYCSLEQPGQGGSTSAFEQLQRSRRRLISQRSSLETLEDIEENAPLRRCRTLSGSPRPKNFKKIHFIKNMRQHDTRNGRIVLISGRRSFCSIFSVLPYRDSTQVGDLKLDGGRQSTGAVSLKEIIGLEGVELGADGKTVSYTQFLLPTNAFGARRNTIDSTSSFSQFRNLSHRSLSIGRASGTQGSLDTGSDLGDFMDYDPNLLDDPQWPCGKHKRVLIFPSYMTTVIDYVKPSDLKKDMNETFKEKFPHIKLTLSKIRSLKREMRKLAQEDCGLEEPTVAMAFVYFEKLALKGKLNKQNRKLCAGACVLLAAKIGSDLKKHEVKHLIDKLEEKFRLNRRELIAFEFPVLVALEFALHLPEHEVMPHYRRLVQSS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be designed to localize specifically to nucleus in order to perform its role in cell division, nervous system development more effectively. The designed protein sequence is ",
    "response#1": "STCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGDVPPGPLPEAILALYNSTRDRVAGESAETEPEPEADYYAKEVTRVLMVEYGNKIYDKMKSSSHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLTRREEIEGFRLSAHCSCDSKDNTLQVDINGFSSGRRGDLATIHGMNRPFLLLR",
    "response#2": "STCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGDVPPGPLPEAILALYNSTRDRVAGESAETEPEPEADYYAKEVTRVLMVEYGNKIYDKMKSSSHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLTRREEIEGFRLSAHCSCDSKDNTLQVDINGFSSGRRGDLATIHGMNRPFLLLN",
    "response#3": "STCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGDVPPGPLPEAILALYNSTRDRVAGESAETEPEPEADYYAKEVTRVLMVEYGNKIYDKMKSSSHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLTRREEIEGFRLSAHCSCDSKDNTLQVDINGFSSGRRGDLATIHGMNRPFLLLN"
  },
  {
    "reference": "MSNNKFKVAVVGSTGIVGRTVLKVLEEKNFSNCEYTLFSSSKSAGSRLSFLGQNYIICELTETSFDYHFDFAIFCAGGDVSKKFAPIAVSKGCVVIDNSSVFRMNKDVPLVVPEVNPEKIRENVGIIANPNCSTIQAVVALKPLDLKYGIKRVVYSTYQAVSGAGRQGVLDLENGLKGEPTKKFPYPIVNNCLPHIDSFLDDGYTKEEYKLINETRKILNKPFLPVTATAVRVPVKNCHSECINVEFERDFDVFDVKQLLQNSPGVLVIDDPSKNYYPIASKADGTDDVLVGRIRRDFSVRFGLNLWVVSDNLRKGAASNAVQILEYLISS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should consider the endogenous proteins and enzymes present in the L-threonine from L-aspartate: step 2/5, Amino-acid biosynthesis, L-threonine biosynthesis pathway to avoid interference with native metabolic processes.2. The protein should be able to bind phosphate ligand in a variety of conditions3. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.4. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.5. The designed protein should be optimized for NAD binding activity in order to improve threonine biosynthetic process, diaminopimelate biosynthetic process, isoleucine biosynthetic process. The designed protein sequence is ",
    "response#1": "IALVGASGVVGAEFLRTMEERETPVKELRLLATSRSAGQTRRFRGEELTIEETTPAAFEGADVAFISASTAASQELCPIARDAGAVAFDDSSAFRQAPGVPLVVPEVNGVDLAATPGVLAARRVDGICVRVGAMRCHLWPLMAERTIRRVIADTYQSTSGAGTAAMAELTDQIGALMDERPVVIENFPKQIAFNAIPQVDVFQEDGYTKEEWKMLVESRKILHQPALPLSATCVRIPTLVSHAIAVHVDFDAPLAAAEARALWGGQAGLTVVDDPVNGGYPTPLDCAAQDAVFVGRARQDVSSPNGLAFWCVTDNLRLTPGVVNLAPIHRAVGIDLINISTYEAVSGSGRKGVKELAQQTAQLLNAREIESRVYDKQIAFNVIPYVGSFLENGYTHEEMGLVWETQKVLDDTSVRVNPTCVRVPVFFGHSASVYIETLAYISAEEVIDILETTEGVEVLSSVADMSFATPVSDAAGSDAIYVSRIREDLNGRQGISLWITADNVREWSGGAARLAGLIREDLIEWISAMTYQAASGGGARNMRELISQMGVLHHSVEQSLTSADSSILSIDSDITAALRGPSLPTSECGAALAGSLIPWIDAELENGQSREEWKAQAETNKILEKNSAQVIPVDGICVRVGAMRCHSQALTIKLRKDIPLDEIEGILADGNDWVRVITNSKTETVEQLSPAAVSGTLSVPIGRLRKLNMGPDYLSAFTVGDQLLAPERP",
    "response#2": "LAGLIREDLIEWISAMTYQAASGGGARNMRELISQMGVLHHSVEQSLTSADSSILSIDSDITAALRGPSLPTSECGAALAGSLIPWIDAELENGQSREEWKAQAETNKILEKNSAQVIPVDGICVRVGAMRCHSQALTIKLRKDIPLDEIEGILADGNDWVRVITNSKTETVEQLSPAAVSGTLSVPIGRLRKLNMGPDYLSAFTVGDQLLYGDRMDMSCKPIYDAVGIERINVATYQSVSGAGASGVRELEDQTHRSVRGELIDELPVHPAQMAFNVISHAWTMGEDGFSDEETKVVNETRKILEDDRLRVNPTCVRVPVYYGHSMAVHLETRESISPGAACDLLRHAPGVVLMEGAAPTPVVDAAGRDGVFVGRVRGDPSHERGLDLWVVSDNVRVTSPGHDIQAELLAGLIREDLIEWISAMTYQAASGGGARNMRELISQMGVLHHSVEQSLTSADSSILSIDSDITAALRGPSLPTSECGAALAGSLIPWIDAELENGQSREEWKAQAETNKILEKNSAQVIPVDGICVRVGAMRCHSQALTIKLRKDIPLDEIEGILADGNDWVRVITNSKTETVEQLSPAAVSGTLSVPIGRLRKLNMGPDYLSAFTVGDQLLYGDRMDMSAVYLAGLIREDLIEWISAMTYQAASGGGARNMRELISQMGVLHHSVEQSLTSADSSILSIDSDITAALRGPSLPTSECGAALAGSLIPWIDAELENGQSREEWKAQAETNKILEKNSAQVIPVDGICVRVGAMRCHSQALTIKLRKDIPLDEIEGILADGNDWVRVITNSKTETVEQLSPAAVSGTLSVPIGRLRKLNMGPDYLSAFTVGDQLL",
    "response#3": "LAGLIREDLIEWISAMTYQAASGGGARNMRELISQMGVLHHSVEQSLTSADSSILSIDSDITAALRGPSLPTSECGAALAGSLIPWIDAELENGQSREEWKAQAETNKILEKNSAQVIPVDGICVRVGAMRCHSQALTIKLRKDIPLDEIEGILADGNDWVRVITNSKTETVEQLSPAAVSGTLSVPIGRLRKLNMGPDYLSAFTVGDQLLGLTELPVQQPCKPIYDAVGIERINVATYQSVSGAGASGVRELEDQTHRSVRGELIDELPVHPAQMAFNVISHAWTMGEDGFSDEETKVVNETRKILEDDRLRVNPTCVRVPVYYGHSMAVHLETRESISPGAACDLLRHAPGVVLMEGAAPTPVVDAAGRDGVFVGRVRGDPSHERGLDLWVVSDNVRLLPVPVAPYKPIHDAARIRRVIVSTYQATSGAGVAGQRDLEAGTEALMAGHSYEYEAFAHPIAHNLIPQIGSTKYQGYTSEEMKMVYETHKIFEDDSIQVCPTCVRVPVTNCHSESILVETERKVSVDEARQLFEAFPGITVIDDLEQNQYPMPLTCDGHDDVFIGRIREDISSANGLAFWCVSDNLRLLPVPVAPYLAGLIREDLIEWISAMTYQAASGGGARNMRELISQMGVLHHSVEQSLTSADSSILSIDSDITAALRGPSLPTSECGAALAGSLIPWIDAELENGQSREEWKAQAETNKILEKNSAQVIPVDGICVRVGAMRCHSQALTIKLRKDIPLDEIEGILADGNDWVRVITNSKTETVEQLSPAAVSGTLSVPIGRLRKLNMGPDYLSAFTVGDQLL"
  },
  {
    "reference": "MQATALPILSNVSPKPALLDRSLEELVSWLADRGEPALRARQIRRWLVAGRAESFGEMSDLPKTLRPRLVAEFQPFSTAIARQQVSSDGTTKLLLRLHDGRHIECVMIPEGDRRTACISTQVGCGMGCVFCASGLNGVDRNLTVGEMLEQLIRLRNSLPVGERLTGIVVMGMGEPLANLDRLLQALAIACHKDGLNIGARHVTISTVGLPAKIRQLADAKKQYHLAVSLHAPNDALRNRIVPTNEPIGMDAILDAADYFFATTGRQVTYEYVLLGGMNDRPEDAKELSRLLKGRKAHVNLIPFNEVDGLPYRRPHPAEIPLFVSILERHGINVKVRKRKGADIDAACGQLRRRVEEAVSQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand.2. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity.3. The S-methylcysteine intermediate active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "MKDHPTFCISSQVGCAMACGFCATARGGLKRQLTAGEIVEQVLRLNRDLRAEPYPHLGGRLFNVVFMGMGEPLDNWTNVHQALKILMDDAGLAMSRRRIQISTSGPAEGLRALLQDHPGVGLTLSLGGSNDTDRRRIMPVPGRTPVTGAVDLAAQYARRNGRRATLAWVLIADKTDRPEQARELVALARRGPFKVNLIPLNPLDDGRLEPASDQGVLAFQKILTDAGVPAFIRASGGE",
    "response#2": "MKDHPTFCISSQVGCAMACGFCATARGGLKRQLTAGEIVEQVLRLNRDLRAEPYPHLGGRLFNVVFMGMGEPLDNWTNVHQALKILMDDAGLAMSRRRIQISTSGPAEGLRALLQDHPGVGLTLSLGGSNDTDRRRIMPVPGRTPVTGAVDLAAQYARRNGRRATLAWVLIADKTDRPEQARELVALARRGPFKVNLIPLNPLDDGRLEPASDQGVLAFQKILTDAGVPAFIRASGGQ",
    "response#3": "MKDHPTFCISSQVGCAMACGFCATARGGLKRQLTAGEIVEQVLRLNRDLRAEPYPHLGGRLFNVVFMGMGEPLDNWTNVHQALKILMDDAGLAMSRRRIQISTSGPAEGLRALLQDHPGVGLTLSLGGSNDTDRRRIMPVPGRTPVTGAVDLAAQYARRNGRRATLAWVLIADKTDRPEQARELVALARRGPFKVNLIPLNPLDDGRLEPASDQGVLAFQKILTDAGVPAFIRASGGE"
  },
  {
    "reference": "MFSRNFTTPSIRIYLNSCVSHRSFHNTTVSQSWLSRIRDRFRGTKEDEKKQIADKDMAAMKVVIPPKKRVSKWKDIQLFEKFAEPSLNREDSLKMINEIRKSLQLQTPWSPEARLQAVKLAYQKTGRIVYDAPLQNIHNWDDLYNYYDKIVDYGDTTLHGRLAWKPTPGLMSLPNVVLHVDENGYPLERKKRRLSRSVA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be expressed specifically in the mitochondrion The designed protein sequence is ",
    "response#1": "MSFRVKGWSDQFSESFYERATTLLTAALNKGTKHSMIADHITVKELDLGPQPPQLEILEVGDLAPDRFQGLFNLHYSGDASLVLQTKIRANPLSVQKSNVPSFSSRNTMLASRAPLTVPMFLRLSDLKLNGIVVLVFSKQKGITVVFRNDPLESVRVSSSFDSIPAIARFLQREIEVQLVSLFQEELPSIIYKMSL",
    "response#2": "MSFRVKGWSDQFSESFYERATTLLTAALNKGTKHSMIADHITVKELDLGPQPPQLEILEVGDLAPDRFQGLFNLHYSGDASLVLQTKIRANPLSVQKSNVPSFSSRNTMLASRAPLTVPMFLRLSDLKLNGIVVLVFSKQKGITVVFRNDPLESVRVSSSFDSIPAIARFLQREIEVQLVSLFQEELPSIIYKMSC",
    "response#3": "MSFRVKGWSDQFSESFYERATTLLTAALNKGTKHSMIADHITVKELDLGPQPPQLEILEVGDLAPDRFQGLFNLHYSGDASLVLQTKIRANPLSVQKSNVPSFSSRNTMLASRAPLTVPMFLRLSDLKLNGIVVLVFSKQKGITVVFRNDPLESVRVSSSFDSIPAIARFLQREIEVQLVSLFQEELPSIIYKMSP"
  },
  {
    "reference": "MAKIKKKGTSGAAKNYITRTQAVRKLQISLPDFRRLCIFKGIYPREPRNKKKASKTSTPSTTFYYTRDIQYLLHEPLLKRFRDQKALSKKIARSLGRGDVGDAARLEKNNAPKITLDHIVKERYPTFIDALRDLDDALSLLFLFANLPSTANVPPKTIALCQRLCHEFQHYLIATNSLRKSFLSIKGIYYQATIQGQDILWLVPYRFVQQVTGDVDYRIMATFVEFYTTLLGFVNFRLYTSIGLVYPPKFDSRSDERGAELAAFTLEGRKVGEMQKAISSEPQTNGQLAKSDSAANDIQAKVDNILKETTRNNEPDDEAVDVEDNVDAIDKFETTAPEADTLPQPQMSGNEVASLFASFTFFISREAPRGPLEFLLRAFGCKRVGWDAVLGEGAFTHDETDPRITHQIVDRPPLPESALPPLPQNPGEGAEKAPRVRPGTRMPGRMYIQPQWVWDCVNEGKLLRHDLYAPGATLPPHLSPWVKASKGGYDPRLSLAEQESDGEAERQAEEENEEEESEVEGLSMDKEMVETENSEAGESDEESVDGGMDVDIPGSDDEEEESEEDNALRELGGSDEAADVQSESEDDEEAARNQHQMELEAEAAGLPFTGADGTGTSSKKSKKQKPLAKKHAAQKKKEQEELERQKMMMSRKKRKLLDKMLYSNKKKDEEAEKLRRKRRKIEQGTKGR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should emphasize Acidic residues, Basic and acidic residues compositional bias.2. The protein should be optimized for stability and solubility when expressed in nucleolus, nucleoplasm, preribosome, large subunit precursor where it will be involved in maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA).3. The protein contains novel BRCT domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "GQQSSDIIQKLQKDLFMLKKPEAIQYNKKNAFRPMESAESIEFLGEKSNSSLFTFGSHSKKREHNLIMGRLFDNAVFDMIEMGISNFKSIKEFGADGSAVGNKPMFIFQGNEFENNETVKKVGNLFLDMFRGRLVEYINLVGLDHVVIMTAVDDKILFRHYTTIFSPSGSKIPKVDLKEVGPSFDITIRL",
    "response#2": "GQQSSDIIQKLQKDLFMLKKPEAIQYNKKNAFRPMESAESIEFLGEKSNSSLFTFGSHSKKREHNLIMGRLFDNAVFDMIEMGISNFKSIKEFGADGSAVGNKPMFIFQGNEFENNETVKKVGNLFLDMFRGRLVEYINLVGLDHVVIMTAVDDKILFRHYTTIFSPSGSKIPKVDLKEVGPSFDITIRL",
    "response#3": "MFIRGQQSSDIIQKLQKDLFMLKKPEAIQYNKKNAFRPMESAESIEFLGEKSNSSLFTFGSHSKKREHNLIMGRLFDNAVFDMIEMGISNFKSIKEFGADGSAVGNKPMFIFQGNEFENNETVKKVGNLFLDMFRGRLVEYINLVGLDHVVIMTAVDDKILFRHYTTIFSPSGSKIPKVDLKEVGPSFDITIRRR"
  },
  {
    "reference": "MIKLGIIMDPISSIKIKKDTSFSIMLEAQKRNYFLYYIEMKDIYYEVGEVYANSYLISVKYDEKKWYSFKKKYTINLKELDVILMRKDPPFDIEFLYITYILEHIENFGVLIINKPKSLRDYNEKISTLSFKYSPKTLISCSKKAIYSFQEKFGDIILKPINKMGGDSVFYVKKNDPNVSVIIDQLTNYGNSFCLIQEYIKEILNGDRRIIMINGSPLPYCLVRIPNDKEIRGNLAAGASFDILPLRKIDYEISNNISSFLKDKGLIFVGLDIIGNYLTEINITSPTGINEIESVYKVSISGILLDSIEKLLNIKH",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be able to perform metal ion binding, ATP binding, glutathione synthase activity under specific conditions.2. The protein should be optimized for activity in glutathione biosynthesis, Sulfur metabolism, glutathione from L-cysteine and L-glutamate: step 2/2 pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The designed protein should contain one or more ATP-grasp domains that are essential for its function.4. The presence of Mg(2+), Mn(2+) is necessary for the protein to function in the desired environment.5. We need a protein that can perform ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP +   glutathione + H(+) + phosphate with high efficiency.6. The designed protein should have a high specificity for ATP, Mg(2+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSVNGGLVTAQASAFEFLGAKNDDDHAWFKSADKVQTALEAFDAVIMRTDPPFDMQYLYATQLLTLAEQQGAKVFNSGQAMASISRKFFATQFTQCTPPTMVSRRSDILREFAAEHRDIILKPLDGMGGSSIFRHREGDPNLSVILETLTQHGSQQIMAQRYLPEIKDGDKRILMIDGEPVPYCLARIPAQGETRGNLAAGGRGVAQPLSERDRWIAAEVGPHLRERGLLFVGLDVIGDYLTEINVTSPTCIREIDQAFDTRIGDKLMDAIDSFYGAIMEKFFATQFTQCTPPTMVSRRSDILREFAAEHRDIILKPLDGMGGSSIFRHREGDPNLSVILETLTQHGSQQIMAQRYLPEIKDGDKRILMIDGEPVPYCLARIPAQGETRGNLAAGGRGVAQPLSERDRWIAAEVGPHLRERGLLFVGLDVIGDYLTEINVTSPTCIREIDQAEYTLNNLRFALTVEPDNFDLQQRYQAVAIARQQGQATIPSRLDIEKATNPFLRWDQAALQAAVSSQDPVQTLARLRSRKDQLWSEKLFTAWFPELTPITMVTRKAEKIKAFREQHGDVILKPLDGMGGASIFRVKEGDPNLSVIIETLTNHGQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGGRGEPRPLSETDKKIALAVAPTLKEKGLLFVGLDVIGDKLTEINVTSPTCIREIEA",
    "response#2": "MTTLYDSKNAPVLTGIMRGIEREGLRIDRDGKLAQTPHPVALGSALTHPQITTDFSEALLEFITPPTHRVEDLFKQLYDIQGYTLSKLDDELIWSSSMPCVLNDDATIPVAQYGSSNNGTMKTVYRVGLGHRYGRAMQTVAGLHYNFSLPDAFWSFLHREEYSLLDLQDFKDQKYFALIRNFRRYYWLLVYLFGASPALCGSFIKGREHNLQPLIDERTLHLPYATSLRMGDLGYQSSAQESLYVCYNDKQSYITTLCAAITTPIDEYKAIGLQDEKGEFKQLNTSLLQIENEFYSSIRPKRTAKHGETALSALRNRGVEYIEVRCLDIDPFDPLGVNKPQVRFLDTFLLYCALQDSPDTSPEESANILRNQKTEKLFTAWFPELTPITMVTRKAEKIKAFREQHGDVILKPLDGMGGASIFRVKEGDPNLSVIIETLTNHGQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGGRGEPRPLSETDKKIALAVAPTLKEKGLLFVGLDVIGDKLTEINVTSPTCIREIEADGSMVEEKLFTAWFPELTPITMVTRKAEKIKAFREQHGDVILKPLDGMGGASIFRVKEGDPNLSVIIETLTNHGQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGGRGEPRPLSETDKKIALAVAPTLKEKGLLFVGLDVIGDKLTEINVTSPTCIREIEAAYDISITGKLMDAIDGSMVERAVVIKPKSTNYGLGISIFQQGVHDREDFAKAIEIAFREDKEVMVEDYLTGTEYRFFVLGDETLAV",
    "response#3": "MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSVNGGLVTAQASAFEFLGAKNDDDHAWFKSADKVQTALEAFDAVIMRTDPPFDMQYLYATQLLTLAEQQGAKVFNSGQAMERAVVIKPKSTNYGLGISIFQQGVHDREDFAKAIEIAFREDKEVMVEDYLTGTEYRFFVLGDETLAVIEYTASNVKFALTIEPDNAALQARAKQVEQQRAAGQPTIPVTLGEEKQANLFLRADVPSVAAAVGLPGESAADVFGELRERKNWSEELKW"
  },
  {
    "reference": "MENRWQVMIVWQVDRMKIRNWHSIVKHHMYASKKAGKWFYRHHYESRHPKVSSEVHIPLGEAKLVVTTYWGLHTGEKPWHLGHGVSIEWRQKSYSTQVDPDMADQLIHIYYFDCFSESAIRKALLGQIVRPSCEYQTGHNKVGSLQYLALKALVKPKKRRPPLPSVRKLTEDRWNKPQKTKGHRENPTMNGHWNC",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that In turn, they are directed to the 26S proteasome for degradation; May interact directly with APOBEC3G mRNA in order to inhibit its translation; Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G; Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions; Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism2. The protein can be targeted to virion component, plasma membrane, cytoplasm and perform RNA binding.3. The protein design must feature the HCCH motif motif. The designed protein sequence is ",
    "response#1": "MEPVDPSLEPWNHPGSQPKTACNNCYCKRCCWHCQLCFLKKGLGISYGRKKRKHRRGTPQSSKDHQNPVPKQPLSTSRGNPTGPEESKKKVASKTETDPCA",
    "response#2": "MEPVDPSLEPWNHPGSQPKTACNNCYCKKCCWHCQLCFLKKGLGISYGRKKRKRRRGPPQSSKDHQNPIPKQPSSQPRGNPTGPKESKKEVASKTEMDP",
    "response#3": "MENRWQVMIVWQVDRMRIRTWKSLVKYHMYVSKKAKDWVYKHHYESRHPKVSSEVHIPLGDARLVIKTYWGLQTGERDWHLGHGVSIEWRQRTYNTQVDPDLADQLIHLYYFDCFSESAIRKAILGEVVSPECEYQAGHNKVGSLQYLALTALIKPKKRKPPLPSVKKLTEDRWNKPQKTKGHRGNHTMNGH"
  },
  {
    "reference": "MNDMAKNLILWLVIAAVLLTVFNNFSVDSSPQTINYSQFVQQVQNNRIRSVTIDGYTITGEREDGSSFQTIRPSAQDPKLMDDLLANNVTVVGKKPEEQSLWTRLLIASFPILIILAIFLFFMRQMQGGAGGKGGPMSFGKSKAKLLSQDQIKTTFADVAGCDEAKEEVEELVDFLRDPTKFQRLGGTIPRGVLMVGPPGTGKTLLARSIAGEAKVPFFSISGSDFVEMFVGVGASRVRDMFEQAKKQAPCIIFIDEIDAVGRSRGTGMGGGNDEREQTLNQLLVEMDGFEANEGIIVIAATNRPDVLDPALLRPGRFDRQVTVGLPDIRGREHILGVHLRKVPLGDDVKPSLIARGTPGFSGADLANLVNEAALFAARRNKRLVSMEELELAKDKILMGAERKSMVMTDKEKLNTAYHESGHAIIGLVMPEHDPVYKVTIIPRGRALGVTMFLPEQDRYSLSRQQIISQICSLFGGRIAEEMTLGPNGVTTGASNDIKRATELAHNMVAKWGLSDEMGPLMYDEDESHQFMGMGSQGGMLKSGETTTRLDKEVRRIIDECYAEAKRILEENRDKLDIMAEALMQYETIDATQLKDIMEGRKPRPPADWDGGDASGGSTISQAPDQEPQASQSDEGDEADDDKPRRRPSDPLGGPAGH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be able to bind ATP, Zn(2+) ligand in a variety of conditions2. The ideal protein would be one that plays a critical role in proteolysis, protein catabolic process through ATP-dependent peptidase activity, metalloendopeptidase activity within plasma membrane.3. Target a Polar residues compositional bias in the protein's composition for improved properties.4. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins; Plays a role in the quality control of integral membrane proteins.5. The presence of AAA+ ATPase domains is critical for the interaction of the designed protein with its intended targets.6. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "MVMSEEEKKLTAYHEGGHALVALHMEDSDPVHKATIIPRGRALGMVMRLPERDQLSVTRSKLKADLAVAMGGRVAEEVIFGHDKVTSGASSDISMATKMAKAMVTQWGMSDKLGPLAYQDNEEEVFLGHSVARQQTMSEDTARMIDEEVKRIVSEGYEKAQELLTKHLDELHTLAQGLLEYETLSGEEIKAEMRMQQARDMARRKEVLLVGPPGTGKTLLGKAVAGEAGVPFFSLSGSDFVEMFVGVGAARVRDMFQQAEQRAPCIVFIDELDALGKTRGSGNMGGHDEREQTLNALLVEMDGFGTNSGIIIMGATNRPETLDPALMRPGRFDRHVLVADIQSYDRVTILQIHAQKQGFDLPENVSLSIIARRTPGFSGADLANLLNEAAILTARRRKDAITLNEIENAIDRVIGFKSRNRVIVEKEKLIVAYHEIGHALVQWVLPYTEPVHKVTTIPRGMAALGYTMTLPTEDRYLKSKNEYLSEIRTLLGGRASEEVVFGDITTGASNDIERATAIAHAMVTKFGMSEKFGPILLDNTNDGDLFMQKHYSETTGKEVDDEVRTLITEAYEDSKKILRDNYEKLEKVTRALLDRETISGIEAFRAKAEAA",
    "response#2": "MVMSEEEKKLTAYHEGGHALVALHMEDSDPVHKATIIPRGRALGMVMRLPERDQLSVTRSKLKADLAVAMGGRVAEEVIFGHDKVTSGASSDISMATKMAKAMVTQWGMSDKLGPLAYQDNEEEVFLGHSVARQQTMSEDTARMIDEEVKRIVSEGYEKAQELLTKHLDELHTLAQGLLEYETLSGEEIKAERWRSEQLARGIPKGVLMVGPPGTGKTLLARAVAGEAGVPFFHISGSEFVEMFVGVGASRVRDLFEKAKAAAPSIVFVDEIDAVGRQRGAGLGGGHDEREQTLNQILVEMDGFDNQTNVIVIAATNRADILDPALLRPGRFDRKVFVDLPGLEERERLVALHLDAHRQPLSQDVDLRIIARGTPGFSGADLANLVNEAALLAAKIGKRFVGMEDFTRALAA",
    "response#3": "MVMSEKEKLNTAYHESGHAIVGRLMPEHDPVYKVSIIPRGRALGVTMFLPEEDKYSHSKRYLDSSICSLFGGRIAEELTLGFEGVTTGASNDIERATSLARNMVTRWGLSEKLGPLQYDTDSDEPFLGRSAGQAQTVYSPETAQRIDEEVRNIIDTCYEKAKQILVDNRDKLDLMADALMKYETIDRFQIDDRLEEELRR"
  },
  {
    "reference": "MGNQLAAATVVNVSDVTSSLELIAPLSCMHRNYFRSFRCSKEASAAVPSDTLRSLYSFSRGDNRDQSALHHRPSNRGGSTEVVLKVFVRTLYNEEEQKVVEFCYNHLLSIERRMAGAKPANMQNTGSCETAASNGSSHDSSNCGVATTPPPCNVLFYSCMEIQNDRFCMLQRPYVAFSLAERLATRPYWSTANRLFATYQLLQGTVQLHEVWGTVHGDLKVDNILVNTSGWLYITDICPFNPALLPANNPALFNYFYDTNESHICSLAPEKFVDHPTNVNTSNLNATVHTPGMDIFSLACVVVYIFTEEPLFRLSEVLELRSLQTQREREEMINSILQERGLAPNLCRMLSDMLCTNPNSRPTSRQLLERYTPTVFPPYFEYLYRDILPALLPRPPEHQVQLLWSRLDDIFAEVECRGSFNSGGGSGHNGEADDGEFPPLVPSSDAKKVTVLVLIPIILNAGLHLMASETCCRVVSIVQRILPHCTLEVQADVILPYMLRFLRTETLPVVARVLALRTLSTICHNIPFSTGEATVFDDLILPCVEDLVAKADTQNVTLLLEVAAQLPSLFLMARSFMEQQQALVTSNERQSTYGEQLNRLLERGWDALRTLYKHNHSAVVIAALQRSVDVVKFLGEERTQHDFIPFLTTAIASTIDVQRELYPQAILCHTNLQRPPLKTLRFFVEEALRRTDAIILRRAIESVSVVASSKLVDVSDVMSLAECVLPHILDKDKWVSLAARQLVEVVAQTYRMSDVCTRLPSTLIPLLR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be involved in macroautophagy, intracellular protein transport, cell morphogenesis by localizing to cytoplasm, phosphatidylinositol 3-kinase complex, class III, type II and exhibiting protein serine kinase activity, 1-phosphatidylinositol-3-kinase activity. The designed protein sequence is ",
    "response#1": "GPTTQKDYLEVMTQVHEEGFELGTLAGPAFARLRKSIGLTEEDYQATLGPGDPYLQFFSTSKSKASFFLTHDQRFFVKTQRRHEVHVLLAHLPRYVEHLQQYPHSLLARLLGVYSLRVAQGKKKYFIIMQCIFYPTSRISERYDIKGCNISRWVDPAPEGSPLVLVLKDLNFQEKTMRLGAQRSWFLRQMELDTAFLREVNVLDYSLLVAIQFLHEDEKGIHHSVFSTFKSIQGVSKSKGTGDQNCRMLPDLPNALHILDGPDQRYFLGLVDMTTVYGFRKRLEHVWKMVRYPGQSVSTVSPAHYARRC",
    "response#2": "GPTTQKDYLEVMTQVHEEGFELGTLAGPAFARLRKSIGLTEEDYQATLGPGDPYLQFFSTSKSKASFFLTHDQRFFVKTQRRHEVHVLLAHLPRYVEHLQQYPHSLLARLLGVYSLRVAQGKKKYFIIMQCIFYPTSRISERYDIKGCNISRWVDPAPEGSPLVLVLKDLNFQEKTMRLGAQRSWFLRQMELDTAFLREVNVLDYSLLVAIQFLHEDEKGIHHSVFSTFKSIQGVSKSKGTGDQNCRMLPDLPNALHILDGPDQRYFLGLVDMTTVYGFRKRLEHVWKMVRYPGQSVSTVSPAHYARRC",
    "response#3": "GPTTQKDYLEVMTQVHEEGFELGTLAGPAFARLRKSIGLTEEDYQATLGPGDPYLQFFSTSKSKASFFLTHDQRFFVKTQRRHEVHVLLAHLPRYVEHLQQYPHSLLARLLGVYSLRVAQGKKKYFIIMQCIFYPTSRISERYDIKGCNISRWVDPAPEGSPLVLVLKDLNFQEKTMRLGAQRSWFLRQMELDTAFLREVNVLDYSLLVAIQFLHEDEKGIHHSVFSTFKSIQGVSKSKGTGDQNCRMLPDLPNALHILDGPDQRYFLGLVDMTTVYGFRKRLEHVWKMVRYPGQSVSTVSPAHYARRC"
  },
  {
    "reference": "MESERSCLLSSHDAGKGGSSSVSSASFNFINSIIGSGIIGLPYSMSQAGLPMGLLLLILVAFITDYSIILLVRGGNLSGTHSYQSLVRSTFGQIGYIIVSVLQFLYPFIAMISYNIIAGDTLTKVFMRIPGVGPGNILTERHFVIAMSTVLFTLPLSLYRDIAKLGKVSLLSMILTFGILMTVVVRAATLGPQIPASDDAWVFARWNAIQAVAVMSFALICHHNSFMIYGSLQEPTLSRWSLVTHISVGSSVLVSAVFAAAGYATFTVYTQGDIFENYCRSDNLATFGRFCYGVSIITTFPLECFVTREVISNALFKGGELSKSSHVIITLVIISATTAISLSYDCLGIVLELNGILSAVPLMFIFPSACFLKLSNERWCRGENLIASMILVAGVFVMIIGLIMMALFPQDCSHGAEMFYCSASNTSSTASPSNILQFINTTQNTSIV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be expressed specifically in the membrane The designed protein sequence is ",
    "response#1": "RCLNLGSYNYLGFAAATGQCADDSEESARSSGLAYCSSRCELGDNEQLQELEALTARYFGVEDAIVFGMGFATNALNLPSLLGPNSLVISDEKNHASIILGLRLSGATTKVFKHNNMRDLERVLRQGVCYGNPKKGGQPWDKVMILVEGIFSMEGSIVRLPEVIALKKKYKAYLYLDEAHSVGAMGSRGRGVTDYFNVDPKEVDILMGTFTKSFGSAGGYLAGSKKLIDFLRTNSHAHCYAASISPPIAQQILTSMKTIMGEDGTDIGRKKIHQLARNTRYFRRRLAQLGVITYGHEDSPVVPMLVYLFSKIGAVVRTLTTRHIAAVGAGFPATPIMEGRIRFCLSAAHTKEQLDFALEALTEY",
    "response#2": "RCLNLGSYNYLGFAAATGQCADDSEESARSSGLAYCSSRCELGDNEQLQELEALTARYFGVEDAIVFGMGFATNALNLPSLLGPNSLVISDEKNHASIILGLRLSGATTKVFKHNNMRDLERVLRQGVCYGNPKKGGQPWDKVMILVEGIFSMEGSIVRLPEVIALKKKYKAYLYLDEAHSVGAMGSRGRGVTDYFNVDPKEVDILMGTFTKSFGSAGGYLAGSKKLIDFLRTNSHAHCYAASISPPIAQQILTSMKTIMGEDGTDIGRKKIHQLARNTRYFRRRLAQLGVITYGHEDSPVVPMLVYLFSKIGAVVRTLTTRHIAAVGAGFPATPIMEGRIRFCLSAAHTKEQLDFALEALA",
    "response#3": "RCLNLGSYNYLGFAAATGQCADDSEESARSSGLAYCSSRCELGDNEQLQELEALTARYFGVEDAIVFGMGFATNALNLPSLLGPNSLVISDEKNHASIILGLRLSGATTKVFKHNNMRDLERVLRQGVCYGNPKKGGQPWDKVMILVEGIFSMEGSIVRLPEVIALKKKYKAYLYLDEAHSVGAMGSRGRGVTDYFNVDPKEVDILMGTFTKSFGSAGGYLAGSKKLIDFLRTNSHAHCYAASISPPIAQQILTSMKTIMGEDGTDIGRKKIHQLARNTRYFRRRLAQLGVITYGHEDSPVVPMLVYLFSKIGAVVRTLTTRHIAAVGAGFPATPIMEGRIRFCLSAAHTKEQLDFALEAID"
  },
  {
    "reference": "MTTPHRSLLLDSLDQLDNPRVLVLGDLILDEYIWGTAERVSQEAPSLVMKTDRREHRLGGAANVCHMLRNLGAAVSCAGVIGDDSAGRQLRRQLRANGISDQLVICDPTRPTTCKQRFLATHGTQSAQQILRVDQEEQASISASLQTQLIEQIRSCIXSHDVLVISDYGKGFCPAPLVRAALSAAHQRGLASLVDPRADRDFSRYRGATLIKPNRWETELTTATRIRMPQDALRIGVELCQQYQFSQAVITLDRDGMALVDAQGNGNLFTAHSATVTELTGAGDMVLAVLSVCLASDIAPQVGAQLANLAAGLXVTRQGPSSIGCDDLRRELLAHDPWQHPKNLGLPGLIEHLGRHRLEGKKIVFTNGCFDLLHAGHVACLSEAASLGDILVVGMNSDSSVGQLKGPDRPLISERERAAMLAGLAVVDYVTVFDELTPHALLSVIRPDLLVKGGTYSIDEVVGKEIVESYGGSVAVTRTVDGTSTSRILNRIHGLASTSSRVLQDKPHPRDCSTADADVTEGKREHVPTCTEQLTRTDALRDSQTVHH",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. We need a protein that can perform ATP + D-glycero-beta-D-manno-heptose 7-phosphate = ADP + D-   glycero-beta-D-manno-heptose 1,7-bisphosphate + H(+) with high efficiency.2. The protein must have high specificity towards ATP binding, heptose-1-phosphate adenylyltransferase activity, phosphotransferase activity, alcohol group as acceptor.3. The ATP binding site should be located in a region of the protein that is accessible to the ligand.4. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7- phosphate at the C-1 position to selectively form D-glycero-beta-D- manno-heptose-1,7-bisphosphate.. The designed protein sequence is ",
    "response#1": "MRVKGLRALVIGDLMLDEYLWGKAERISPEAPVQVVDVRREDMRLGGAGNVVNNLVAFGACVSICSVIGGDENGTVLRHAFTGKGVDVSGVFEDPMRMTSKKTRVLAANQQIVRIDRETREEISPEFEEMILAHVRDTRNIWDLFLISDYGKGLLTSRVLSGVIASAQERGIPVIVDPKGSDYRKYRGATIITPNRKEAEVASHIAITDAESLNRAADFLLAEGEYQALLITRSSEGMSLFLNAGEEVHIPTVAREVYDVTGAGDTVVATFGVGLACGLSFERAAQLANVAAGIVVGKVGTSTVSPEEIIGHLGRDNAEAAPQNFAGMLHRAREIKQAGGKVVFTNGCFDILHYGHAHLLQYARSQGDFLILGLNTDASVRRFKGPSRPFVSEDQRAYMLSLYPFVDLIVLFDDDTPLNLIEAIRPDVLVKGGDYTPDTVVGRDVVESHGGRVAICPRLEGLSTTDLVRKIL",
    "response#2": "MRVKGLRALVIGDLMLDEYLWGKAERISPEAPVQVVDVRREDMRLGGAGNVVNNLVAFGACVSICSVIGGDENGTVLRHAFTGKGVDVSGVFEDPMRMTSKKTRVLAANQQIVRIDRETREEISPEFEEMILAHVRDTRNIWDLFLISDYGKGLLTSRVLSGVIASAQERGIPVIVDPKGSDYRKYRGATIITPNRKEAEVASHIAITDAESLNRAADFLLAEGEYQALLITRSSEGMSLFLNAGEEVHIPTVAREVYDVTGAGDTVVATFGVGLACGLSFERAAQLANVAAGIVVGKVGTSTVSPEEIIGLIESGSVATPKSEKAKVKGETIVMTNGCFDILHNGHVNYLSQAAQLGDRLLVAINTDESVARLKGPGRPANHLKSRANVLAALRAVDWVVAFDEDTPTRIIKDCAPDFLVKGGDNDPDKIPGAAEVRAQGGEVLVMDYVEGFSTTHTIERHP",
    "response#3": "MPQFQQAKLLVVGDVMLDRYWHGAASRISPEAPVPVVQVGNQEDRPGGAGNVALNIAALGSAATLVGLVGKDDNAADLESRLTAAGVYCEFLQSVDKPTITKLRVIHQHQQLIRLDFEEIFDSADVADLHSKVTSLISSAQVLVLSDYAKGALQNVSEIIELGRKQNIPVIVDPKGSDFEKYRGATLITPNFSEFEAVVGHCKNEDELVSKGHALLSNLNLQAMLITRGEHGMTLIRPDSPELHLPARAQEVFDVTGAGDTVISVLAAALAAGDGFADATAIANLAAGLVVGKLGTAAISGPELRRAREWQKKGETVVFTNGCFDILHRGHVLYLQQAAMLGQHLVVGLNGDDSVRRLKGETRPLVKQEDRAVLLSALACVDEVVVFSEDTPKELLEVLHPDILVKGGDYKADEVIGRESVKRVEIISFEEGYSTTGLVQKILA"
  },
  {
    "reference": "MSTNISPEALAARAALDVIGTVEPRIAAAIRAELDDQRSALKLIASENYASPAVLLAMGNWFSDKYAEGTVGRRFYAGCANVDTVEAIAAEHARTVFGAAHAYVQPHSGIDANLVAFWAILADRVEAPALRRAQARHVNDLNDDDWAALRRELGDQRLLGMSLDAGGHLTHGFRPNISGKMFQQRAYGVDPNTGRIDYAALRQTAREFRPLILLAGYSAYPRRINFATMREIADEVGAVLMVDMAHFAGLVAGQVLTGDDDPVPHAHIVSTTTHKTLRGPRGGMVLCQPELADQVDRGCPMVLGGPLPHVIAAKAVALAEAGTADFRTYAQQVVDNATALADGLKRRGVTLVTGGTDNHLALIDVSAHGLTGRQAEAALLNAGIVTNRNAIPQDPNGAWYTSGIRIGTPALTTRGLGTNEMDHIADLIHTVLANTQPTADSKAKYTLDPALAAKLASRAAELLTPYPLYPTINLNTDADAHPQNHPRGDATEA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that can modulate the overall flux of metabolites through the tetrahydrofolate interconversion, One-carbon metabolism pathway, enhancing or suppressing its activity as needed.2. The designed protein should have pyridoxal phosphate binding, glycine hydroxymethyltransferase activity, methyltransferase activity within cytoplasm to regulate tetrahydrofolate interconversion, methylation.3. The designed protein must have at least one Serine hydroxymethyltransferase-like domain.4. The (6S)-5,6,7,8-tetrahydrofolate binding site should be located in a region of the protein that is accessible to the ligand.5. The protein should have a fast turnover rate for (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O =   (6S)-5,6,7,8-tetrahydrofolate + L-serine.6. The protein must exhibit the following characteristics: Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier.7. The protein design should incorporate a binding pocket that specifically recognizes pyridoxal 5'-phosphate to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "LADKILQDAINNELKRQEDHIELIASENYVSEDVLKAQGSVLTNKYGEGYPGKRYYGSCENVDIVEQAAIDRLKLIFGVKYANVQPYSGSVANAAAIASVVPSGGKIMGLSLSSGGHLTHGYKISFSGIFYNSITYELNHEGYLDYDAIEKQALEEKPDLIICGYSAYSRTIDFARFKEIADKSGAKLMADIAHIAGLIAAGVHPSPVGYADIITSTTHKTLRGGRGGIIMTNDEEIAKKMNRWVFPGYQGGPLFHAIAGKAVAFYETLTPMFKEYGAKIVSNAKKFCEAFQDKGVKIISGGTDNHLFMINVLDSYKINGKQAETILEKINITINKNTIPFDTLSPTLASGIRLGTAAMTSRDFDQWDELAEIIAAALDFESTADLEGARVRQMLDYVTCADLSALKEGESTAVCLETPEGHVEGALTCVTWDHYRLSLPAKQAAATVTWLRDLSDGYVSLRLDGAPDYSAMRIPGDLEGARVRQMLDYVTCADLSALKEGESTAVCLETPEGHVEGALTCVTWDHYRLSLPAKQAAATVTWLRDLSDGYVSLRLDGAPDYSAMRIPGSAGVL",
    "response#2": "LAQTDPEIASAVALELKRQRDEIELIASENIVSQAVIEAQGSVMTNKYAEGYPGRRYYGGCQHVDVAENLAIERACKLFGCDFANVQPNSGSQANQGVFQALLKPGDTILGMSLDAGGHLTHGAAPNQSGKWFNAVQYGVRKDTLDVDYDQLEALALEHKPQMIIAGGSAIPRTLDFARFREIADKVGAYLLADIAHYAGLIATGHYPSPFPHVHVATTTTHKTLRGPRGGMIMTNDEAIAKKVNSAIFPGIQGGPLMHVIAAKAVAFGEALQPDFERYQAQVVKNAQAMSDELINGGLDIVTGGTDTHLMLVDLRPKGVKGNVAEKALGRAHITCNKNGIPFDTEKPMITSGLRLGSPAGTTRGFSETEFRQIAGWMCDLLDDLDRPDAVAAVRARVRELTARFPLYPDLEGARVRQMLDYVTCADLSALKEGESTAVCLETPEGHVEGALTCVTWDHYRLSLPAKQAAATVTWLRDLSDGYVSLRLDGAPDYSAMRIPGDLEGARVRQMLDYVTCADLSALKEGESTAVCLETPEGHVEGALTCVTWDHYRLSLPAKQAAATVTWLRDLSDGYVSLRLDGAPDYSAMRIPGE",
    "response#3": "LAQTDPEIASAVALELKRQRDEIELIASENIVSQAVIEAQGSVMTNKYAEGYPGRRYYGGCQHVDVAENLAIERACKLFGCDFANVQPNSGSQANQGVFQALLKPGDTILGMSLDAGGHLTHGAAPNQSGKWFNAVQYGVRKDTLDVDYDQLEALALEHKPQMIIAGGSAIPRTLDFARFREIADKVGAYLLADIAHYAGLIATGHYPSPFPHVHVATTTTHKTLRGPRGGMIMTNDEAIAKKVNSAIFPGIQGGPLMHVIAAKAVAFGEALQPDFERYQAQVVKNAQAMSDELINGGLDIVTGGTDTHLMLVDLRPKGVKGNVAEKALGRAHITCNKNGIPFDTEKPMITSGLRLGSPAGTTRGFSETEFRQIAGWICGVLQNMEDCEDLKKVDPQIYEAVISELKRQREHLELIASENFTSLAVLETQGSVLTNKYAEGYPKKRWYGGCEFVDVAEELARKRAMELFGAEYANVQPHSGTQANMAVLFSVLEVGDTIMAMDLACGGHLSHGHPLNFTGRLYKVIPYGVNRDTELLDYEEIRKLALKYNPKLIIAGASAYPRIIDFEAFKDICQEVGALLLVDMAHIAGLVATGLHPNPCNLAEFVTTTTHKTLRGPRGGLILCKKEFAKKLDTNVFPGIQGGPLMHVIAAKAVALKEAQSPQFKEYQENIVKNARALAQALKERGYRIVSGSTDNHLLLVDLRDKGLTGKEAQKILEEVNITVNKNLIPFDPQSAFVTSGIRLGTPAITTRGMKESQMYKIAELIADVLDNPNDEAKLADVRAEVKKLCAEFPLYPNLSY"
  },
  {
    "reference": "MTVKIYGSGGSGTVALPSSKSFTQRYILGSVFLNKSVTLNYVTITGDDSIALDIAQRAGANITMNDSSIKIRTSFTCPSDIYVGESATSYRIALGLLGSAGCITHVKGDPDLAKRPMDPLVKALEANDVHLKLNEDGFYDVDGSESKKRYIEVDGVSSQFVSSMIFYYARKGGGEFLVKNMRSPGYVYITKRVLYDLGYFVNIEKTITINPSGVWKTAIDVEPDYSSLAFFLVLGLLSENVDVRFNIKRMSRIQPDSVLLDMFKDNIAIDRDTLRVLPGIRDRITVDADHNPDLCPPISVIGIFSEHGVEIDNYARLKTKESNRYEGIIDMASRFGAIVEDNGKDLFIKRGDLKDPGTLSYTDHRMIMSAAVAAAASGFEVEIENETKVSKSFPGFFKELSKFANVSESN",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in aromatic amino acid family biosynthetic process.2. The designed protein must have a Proton donor, Proton acceptor active site that is highly conserved among related enzymes.3. A protein should be able to manipulate the activity of enzymes in the Metabolic intermediate biosynthesis, chorismate biosynthesis pathway, either by activating or inhibiting their function.4. The designed protein should be able to compete with endogenous proteins or inhibitors for 3-phosphoshikimate, phosphoenolpyruvate binding.5. For general function, the protein need meet that Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. The designed protein sequence is ",
    "response#1": "TMPASKSFAQRAILAAALAEGTSHLGGYSPCGDSEAALALAGALGAKVRKEGSTLAITGIGARCRQLQPGTVDTGESGLLTRLTIPVLAAVGDGSFTVTGRGTLLRRPLSGAADIMAAFGVLLASEAEGAGKEVRIPATVKGHLIPGTAEVPGQGGSQLISGLLMALPLCDKDSLLRVSEPKSIPYMYITLDVLRHFGIQTRSEMEGDAEMLEADDWSACTGINFKVRGRQHYRAADFDIEGDWSAAANLLVAGAVFGSAELVGMDTKSLQADLTIIDILVEAGAVVSQLEEGTVCVRKAPLEAFQQDLNNAPDLFPIVAVLAAFCAGESRIAGVGRLATKESNRAEAILQMLLQMGVEARVEGDELVVWGESLAARLLGGRLLKGGDYTSRHDHRMVMALKVASLGAESPIIIDDEACVGKSFPDIAIDGPSASGKGTVAERVAAALGYHYLDSGSLYRLTALAAIRAGVSLDDEPGVADLAGSLPAVFEGGRILLAGDDVTDAIRHEEVSAGASKVAALPAVRAALLARQRAYRRFPGLVADGRDMGSVVFPNAVAKVFLTASAQVRAERRHKQLIAKGMPANMHALLQDLQERDARDAQRSVAPLRQCDDADLVDTTPMNIDAAVDAVMTIVETLD",
    "response#2": "TMPASKSFAQRAILAAALAEGTSHLGGYSPCGDSEAALALAGALGAKVRKEGSTLAITGIGARCRQLQPGTVDTGESGLLTRLTIPVLAAVGDGSFTVTGRGTLLRRPLSGAADIMAAFGVLLASEAEGAGKEVRIPATVKGHLIPGTAEVPGQGGSQLISGLLMALPLCDKDSLLRVSEPKSIPYMYITLDVLRHFGIQTRSEMEGDAEMLEADDWSACTGINFKVRGRQHYRAADFDIEGDWSAAANLLVAGAVFGSAELVGMDTKSLQADLTIIDILVEAGAVVSQLEEGTVCVRKAPLEAFQQDLNNAPDLFPIVAVLAAFCAGESRIAGVGRLATKESNRAEAILQMLLQMGVEARVEGDELVVWGESLAARLLGGRLLKGGDYTSRHDHRMVMALKVASLGAESPIIIDDEACVGKSFPDPVSALSGEITICGDKSMSHRALLLAALAEGQTEIRGFLPCADCLATAQALRALWVDIQREKEIVTIRGVGFLGLQPPKAPLNMQNSGTSMRLLAGILAAQRFESVLCGDESLEKRPMQRIITPLVQMGAKIVSHSNFTAPLHISGRPLTGIDYALPLPSAQLKSCLILAGLLADGTTRLHTCGISRDHTERMLPLFGGALETQKEQIIVTGGQKLHGCVLEIVGDLSAAAFFMVAALIAPRAEVVIRNVGINPTRSAIITLLQKMGGRIELHHQRFWGAEPVADIVVYHSKLRGITVAPEWIANAIDELPIFFIAAACAEGTTFVGNLSELRVKESDRLAAMAQNLQTLGVACDVGADFIHIYGRSDRQFLPARVNSFGDHRIAMSLAVAGVRAAGELLIDDGAVAAVSMPQFR",
    "response#3": "TMPASKSFAQRAILAAALAEGTSHLGGYSPCGDSEAALALAGALGAKVRKEGSTLAITGIGARCRQLQPGTVDTGESGLLTRLTIPVLAAVGDGSFTVTGRGTLLRRPLSGAADIMAAFGVLLASEAEGAGKEVRIPATVKGHLIPGTAEVPGQGGSQLISGLLMALPLCDKDSLLRVSEPKSIPYMYITLDVLRHFGIQTRSEMEGDAEMLEADDWSACTGINFKVRGRQHYRAADFDIEGDWSAAANLLVAGAVFGSAELVGMDTKSLQADLTIIDILVEAGAVVSQLEEGTVCVRKAPLEAFQQDLNNAPDLFPIVAVLAAFCAGESRIAGVGRLATKESNRAEAILQMLLQMGVEARVEGDELVVWGESLAARLLGGRLLKGGDYTSRHDHRMVMALKVASLGAESPIIIDDEACVGKSFPDIAIDGPSASGKGTVAERVAAALGYHYLDSGSLYRLTALAAIRAGVSLDDEPGVADLAGSLPAVFEGGRILLAGDDVTDAIRHEEVSAGASKVAALPAVRAALLARQRAYRRFPGLVADGRDMGSVVFPNAVAKVFLTASAQVRAERRHKQLIAKGMPANMHALLQDLQERDARDAQRSVAPLRQCDDADLVDTTPMNIDAAVDAVMTIVEAAASTAVIAIDGPSASGKGTVAARVAAALGYNYLDSGALYRLTALAAKRVGIDWADEAAVATVAASLNIEFSENDVRMAGESVGDAIRTEDISSGASQVAALPAVRAALLFRQRVFNSGHGLVGDGRDMGSVVFPLAPLKVFLTASAEVRAERRYKQLKEKGFSANLADLLLDLKQRDERDAQRSVAPLRQEADARLLDT"
  },
  {
    "reference": "MPVTPLEPRRPWKGKRIVLGVTGGIAAYKAIQVARDLTRLGGEVDVVLTRSAERFVASLTFEGVTGRPVLTDLFSAKGVALHVRLGRDADVVAVAPATADFLARAAQGRADDLICTTLLATSAPVVICPAMNDKMYAHPQVQDNLDHLQNRLGYQIAGPVRGALAVSEGVGRGRLVEPWQIEEAIGRGLAMDDAFMGKSVLVTAGPTQEPIDPVRYIGNHSSGRMGYAIAQAAWRRGAKVTLVSGPSALDNPFGVDTVAVETAHDMHVAVAERIGDSDLNIFSAAVADFRPTDSRDVKLKRDESADRILLDLTENPDVARDTRAKRKKGSVNVGFALETEHLVSNALAKLESKAFDLVVANDANEEGAGFSVRTNRVTLVLKDAQSERLPIMSKDEVAEEILDRARALVIESQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.2. The designed protein must possess phosphopantothenate--cysteine ligase activity to support coenzyme A biosynthetic process under certain conditions.3. The protein design should take into account the physiological conditions of the Cofactor biosynthesis pathway.4. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.5. The presence of Mg(2+) is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "MNIVVGITGGIAAYKAVSVVRSLVLAGHDVHVVPTESALRFVGRPTLEAISRNPVHVDLFEGVAEVRHVAIGQAADLIVIAPATANSIAKLATGLADDLLGNTVLASEAPVVVAPAMHTEMWRHPATQANIATLRDRGVTIVGPAVGQLTGADSGPGRLEEPDVIVAALAAALTTNTLAGRHLVITAGPTREALDPVRFISNHSSGKMAFALAAEAVSAGARVTLISGPVPLSTPDRVTRIDVESANEMQQATNAHIEDADVFIGVAAVADYRPAEISPEKIKKTDDVLQLKLVKNPDIISEVAALKSRPLVVGFAAETSDIVKNGRQKLQEKNLDLLFANKATDTFNSETIAVTVVWPDGTREELPRMSKDEVAERILDEVVRLREQ",
    "response#2": "MNIVVGITGGIAAYKAVSVVRSLVLAGHDVHVVPTESALRFVGRPTLEAISRNPVHVDLFEGVAEVRHVAIGQAADLIVIAPATANSIAKLATGLADDLLGNTVLASEAPVVVAPAMHTEMWRHPATQANIATLRDRGVTIVGPAVGQLTGADSGPGRLEEPDVIVAAIEATLGRADLKGKKVVVTAGPTRERIDPVRYISNFSSGKMGYSMAEAAASLGAETILISGPVSLDKPAGVKVVNVESAADMFEAVRAEYEDATIVIKSAAVADYRPAEIHTQKIKKSDDDSVIELARTTDILQTLGEWKEDQILVGFAAETENVIENGKGKLQRKNLDFIIVNDVTDPGGGFGSDTNVVTLLTRDGRELDLGPTSKDEVAGAIWDTVLALR",
    "response#3": "KKIILGITGSIAAYKAALLVRLLRKQDAQVRVVMTTAAKEFVGELTFSNLSQKPVFSGLWDGSWTEHVHLGGWADLMVVAPASANTLGKMAHGLCDNALTAVYLSAKCPVLVAPAMDADMYQHPSTLRNLDQLKADGVTVLDAGTGFLASGLHGPGRMAEPAQILEAIIHELTRSA"
  },
  {
    "reference": "MRTVTVGTRGSTLALAQTRWVVARLKEEWPDTDFRIQTISTKGDRNRESLEAMAQKGDKGFWVKEIEDALLQKRIDIAVHSLKDLPTEQPEGLEVASIPKRVDARDVLIGKEGMKRLADLPQGARVGTSSVRRKAFLRAYRPDLQVIDLRGNIDTRLAALAGDEYDAIILAAAGLIRTEMRHRIDEFVEPDILLPAPGQGALALETRSGPENDLTIEVAYAIHDHGTDDRITAEREFLAGLGAGCMAPVGAHASIKGGVLTLEGWVGALDGSKVIRATSIGDPAECADIGAELAGDMLGQGAQALIDAARE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein with hydroxymethylbilane synthase activity functions is required to enable protoporphyrinogen IX biosynthetic process, peptidyl-pyrromethane cofactor linkage.2. The protein requires dipyrromethane to activate a critical catalytic site.3. The protein design should consider the endogenous proteins and enzymes present in the coproporphyrinogen-III from 5-aminolevulinate: step 2/4, protoporphyrin-IX biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "IGTRKSKLAVIQSEIIREELEKHYPHLEFPIISRDTIGDEILSKALFEFKRQLAKSLWTRELEALLVTNQCRILVHSLKDLPSEMPDGMVIACIPKRSCPLDAIVFKAGSHYKTVADLPPGSVVGTSSIRRRALLARNFPHLRFVDIRGNVGTRLAKLDAPDSQFDCLVLAAAGLFRLGLKDRIAQMLTAPFVYYAVGQGALAVEVRADDKEMY",
    "response#2": "IVGSRKSKLAMAQTELVIASLEKIFPDIKFEIKNVITEGDRNRHVSLAKIGGKGVFVKEIEDELKDGTIDFAVHSLKDVMPILPEELVLGAFPKRVSPYDCLVSRKNLSSLNDLPKGARIGTNSLRRQGQLLSIRPDLKIIPIRGNIDTRLRKIDTEALDGIILAEAGLTRLNIDLSSYHVLDLQNYIMPAVGQGCLAIECRKNDTRIL",
    "response#3": "VKIGTRGSKLAVVQALEAKQKLLDSFPNLSIEIVKIKTSGDKYANANLAEIGGKGLFIKEIEAELLENNIDMAVHSLKDVPAFFSWGLTIPCVLERLSPCDAFISHKHNSLESLPQQATIATSAIRRKVQLLNFRPDLNIVPLRGNVTTRLQNQSFDGIILAEAGLIRLEKHHLITEVLPPKVMLSAVGQGAICIQCRRNDVKG"
  },
  {
    "reference": "MRRKGIFSDIEDVLKDLRKGRMVIVVDDEERENEGDLVFAASKVSPQKINFMAKYGRGLICVSMLGKDLDRLGLKPMVEDSKEVKEAAFTVSVDAKKEITTGISCYDRAVTIKTLIDPKTKKDDLITPGHVFPLRYKEGGVLVRAGHTEASIDLCKLAGLYPAAVICEIMKEDGKMARLPYLIKFAKLHNLKIISIAQIIEYRRKKEKLIKLVAKSKLPTKFGNFNLFAYESMIDGGIHIALVKGNVRGKKDVLVRVHSSCVTGDIFHSLRCDCGDQLENSLKMIENEGRGVLLYMNQEGRGIGLKNKLRAYELQDKGLDTVEANKKLGFPPDLRDYGIGAQILVDLGLSSIKLLTNNPRKIVGLEGYGLRITERVPIEVNPNPVNLRYLKVKKKKLGHIIKNVK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a high affinity for Zn(2+), D-ribulose 5-phosphate.2. The designed protein have GTP cyclohydrolase II domains with specific sequence motifs that are crucial for its activity.3. The designed protein must have a Proton acceptor; for GTP cyclohydrolase activity active site that is highly conserved among related enzymes.4. Ideal protein should have a binding affinity for key enzymes in the riboflavin biosynthesis pathway to enhance the overall efficiency of the pathway.5. The designed protein must possess 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, magnesium ion binding to support riboflavin biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "ADMPTQYGHFKLVPFKQTSTGLEHMALIKGEWKEDEPILVRVHSSCATGDILGSKRCDCGEQLHKAMQMIEEEGKGVLIYMQQEGRGIGLMNKIAAYKLQEEGLDTVEANIHLGFKPDERDYGCGAQMLRHLGVHKMRLMTNNPTKRVGLEAYGLEIVENVE",
    "response#2": "VGESAMPTVFGDFRAVAYRSRLDGTEHLALVLGDVATAGRAEHGALVRVHSECLTGDIIGSLRCDCGAQLEQALQAIAEEGCGAVVYLRGHEGRGIGLGHKIRAYALQEQGLDTVDANLAQGLPVDSRSYGVGAQILGHLGIRRLRLITNNPAKYGGLEGYGLEIADRR",
    "response#3": "ADMPTQYGHFKLVPFKQTSTGLEHMALIKGEWKEDEPILVRVHSSCATGDILGSKRCDCGEQLHKAMQMIEEEGKGVLIYMQQEGRGIGLMNKIAAYKLQEEGLDTVEANIHLGFKPDERDYGCGAQMLRHLGVHKMRLMTNNPTKRVGLEAYGLEIVENVK"
  },
  {
    "reference": "MASADVHMAGGAETETFAFQAEINQLLSLIINTFYSNKEIFLRELISNASDALDKIRFESLTDKSNVNAQPELFIRLVPDKASKTLSIIDSGVGMTKSDLVNNLGTIARSGTKEFMEALAAGATDVSMIGQFGVGFYSAYLVADRVMVTTKHNDDEQYVWESQAGGSFTVTHDTTGEQLGRGTKITLFLKDDQLEYLEERRLKDLVKKHSEFISYPIYLWTEKTTEKEISDDEEEEDNKKEEEGDVEEVDDEDKDTKDKSKKKKKVKEVSHEWVQINKQKPIWLRKPEEITRDEYASFYKSLTNDWEDHLAVKHFSVEGQLEFKAILFVPRRAPFDLFDTRKKLNNIKLYVRRVFIMDNCEELIPEWLGFVKGVVDSDDLPLNISRETLQQNKILKVIRKNLVKKCIEMFFEIAENKDDYAKFYDAFSKNIKLGIHEDSQNRAKLADLLRYHSTKSGDETTSLKDYVTRMKEGQKDIYYITGESRKAVENSPFLERLKKKGYEVLFMVDAIDEYAVGQLKEYDGKKLVSATKEGLKLDDEDDEEAKKRREERKKRFEELCKVIKDILGDRVEKVVVSDRIVDSPCCLVTGEYGWTANMERIMKAQALRDSSMSAYMSSKKTMEINPDNGIMEELRKRAEADRNDKSVKDLVLLLFETALLTSGFSLDDPNTFAARIHRMLKLGLNIDEDAAADEDADMPALDEGAAEESKMEEVD",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should exhibit Acidic residues compositional bias.2. The protein must exhibit the following characteristics: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction; This cycle probably induces conformational changes in the client proteins, thereby causing their activation.3. The protein should be able to bind ATP ligand in a variety of conditions4. The protein design should include a TPR repeat-binding motif to ensure proper function.5. The ideal protein would be one that plays a critical role in protein folding through ATP binding, ATP hydrolysis activity within perinuclear region of cytoplasm. The designed protein sequence is ",
    "response#1": "NKEIFLRELISNASDALDKIRYQALSDPSQLESEPELFIRIIPQKDQKVLEIRDSGIGMTKADLVNNLGTIAKSGTKSFMEALSAGADVSMIGQFGVGFYSLFLVADHVQVISKHNDDEQYVWESNAGGKFTVTLDETS",
    "response#2": "NKEIFLRELVSNAADAADKLRYLALTDDALYEGDGELRVRVSTDKEKGTVTISDNGIGMTRDSVIEHLGTIAKSGTKEFFNNLSGEANKDSQLIGQFGVGFYSAFIVAKKVTVRTRAAGHPANEGVLWESEGEGSFNVETITKNSRGTEIVLHLRDEYSNKEIFLRE",
    "response#3": "NKEIFLRELISNFSDALDKIRYKALSDPSKLESDKDLRIDITPDKENKTLTIRDTGIGMTKADLVNNLGTIARSGTKQFMEALTAGADISMIGQFGVGFYSAYLVADKVTVISKSNDDEQYIWESNAGGTFKVTQDDDGRAIGRGTKMILHLKDEYSNKEIFLRG"
  },
  {
    "reference": "MIDGISQRLLDQTHPLRIHLLGVAGSGMSGIAALLLEMGHDVSGSDRVTSGEVERLQGLGLKFSSPHSGAAVSGVDAVVYSSAIKPDNVALVEARRIEVPCLRRAECLAAILHTRKGVLVAGTHGKTTTSAMCAHVLNNAGAAVSHYVGAEIPVLGANAHWDGGSEYLVAEGDESDGTLDLYRPAYAVVLNIEEEHLDYYQGLQQIVGVFRTLLEQTSGKAIYCREDSTAYELCSQYEGGLSYGWSDADYTAVDVVEGRGVIAFTVFRRGEEMGRVELGIPGRHNVLNALAAIALADDLQLDFSCVTRALSSFAGAKRRFESVYLSPRFHVIDDYGHHPTEITATLQTARSLEPNRIVALFQPHRYSRTERLAAEFGKALQGADLVFVTGIYAASENPIEGVSGMTVLEAVEQHGSTKCVYHEDLSTVHHIVGNALKAGDLLLTLGAGNVHEAGKRIAADLQLVDLLKGEFAESDIEIKIYESMRRHTTMLVGGPAQFWIEPRTFSAFAXAVNFFKERGVPVRVVGRGSNLLVRDGGIRGAVIHPSKGEFGELVVRGNLLTAGAGVRFKKVASFAEDAGLGDFAWMEGIPGNVGGGLRMNAGAMGWETFDQVVSVTFLXQDGEIRTRTREEIEAQYRNVPELHQSYALSAVFRGEASSRDEIREKMVESKSHRRQSQPLAASAGCIFKNPEGIGAGKLIDEMGLKGRGVGMAEVSHEHGNFIVNRGKADAEDVLQLIEEIRLEAKDQRGINLETEIQXVGEDRAVF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in peptidoglycan biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.2. The designed protein must have a active site that is structurally stable and resistant to denaturation.3. The stability of the protein-FAD complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "KRLLDRLGIPTAKWSVVTRDRRELPQNLKLPLILKVPMEGSTFGIVKVERAEEWDAALEKEFAMAGELLVEEYIDGIEITVPIVNGEVLPAIEIKSPHGFYDYDAKYVYKDGHTEYFCPARSLSAEQVADASRQAVKFYLGAGCRDILRVDFIVGKDGVPYMLEGNSIPGCTATSLVPKAAKVSGISFEKMTATLVY",
    "response#2": "LLDRLGIPTAKWSVVTRDRRELPQNLKLPLILKVPMEGSTFGIVKVERAEEWDAALEKEFAMAGELLVEEYIDGIEITVPIVNGEVLPAIEIKSPHGFYDYDAKYVYKDGHTEYFCPARSLSAEQVADASRQAVKFYLGAGCRDILRVDFIVGKDGVPYMLEGNSIPGCTATSLVPKAAKVSGISFEKMTATLC",
    "response#3": "QVLFTPADENDLAYFLRHLPEEIPIYCIGVGSNLIVRDRGLPGVVIRLAPRGFGEVTTDGDTVHAGAAALDKRVAEAAAAADIGGLEFYFGIPGTIGGALRMNAGANGSETKDVLVEARAVSRRGDKIL"
  },
  {
    "reference": "MTSSSLFREGELGHFCLNKQEMLHLNVTNPNSIYIEGKLSFEGYKSFNIHCYVDTGASLCIASRYIIPEELWENSPKDIQVKIANQELIKITKVCKNLKVKFAGKSFEIPTVYQQETGIDFLIGNNFCRLYNPFIQWEDRIAFHLKNEMVLIKKVTKAFSVSNPSFLENMKKDSKTEQIPGTNISKNIINPEERYFLITEKYQKIEQLLDKVCSENPIDPIKSKQWMKASIKLIDPLKVIRVKPMSYSPQDREGFAKQIKELLDLGLIIPSKSQHMSPAFLVENEAERRRGKKRMVVNYKAINQATIGDSHNLPNMQELLTLLRGKSIFSSFDCKSGFWQVVLDEESQKLTAFTCPQGHFQWKVVPFGLKQAPSIFQRHMQTALNGADKFCMVYVDDIIVFSNSELDHYNHVYAVLKIVEKYGIILSKKKANLFKEKINFLGLEIDKGTHCPQNHILENIHKFPDRLEDKKHLQRFLGVLTYAETYIPKLAEIRKPLQVKLKKDVTWNWTQSDSDYVKKIKKNLGSFPKLYLPKPEDHLIIETDASDSFWGGVLKARALDGVELICRYSSGSFKQAEKNYHSNDKELLAVKQVITKFSAYLTPVRFTVRTDNKNFTYFLRINLKGDSKQGRLVRWQNWFSKYQFDVEHLEGVKNVLADCLTRDFNA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be designed to have high stability and activity towards a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =   diphosphate + DNA(n+1).2. The designed protein should contain one or more Reverse transcriptase domains that are essential for its function.3. The designed protein should be optimized for endonuclease activity activity in order to improve proteolysis.4. The protein must exhibit the following characteristics: Reverse transcriptase is multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids; Partial (+)DNA is synthesized from the (-)DNA template and generates the relaxed circular DNA (RC-DNA) genome; After budding and infection, the RC-DNA migrates in the nucleus, and is converted into a plasmid-like covalently closed circular DNA (cccDNA) .; This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3'- to 5'- endonucleasic mode; Encodes for at least two polypeptides: protease (PR) and reverse transcriptase (RT); Neo-synthesized pregenomic RNA (pgRNA) are encapsidated, and reverse-transcribed inside the nucleocapsid; The protease processes the polyprotein in cis. The designed protein sequence is ",
    "response#1": "MGQTKSKIKSKYASYLSFIKILLKRGGVKVSTKNLIKLFQIIEQFCPWFPEQGTLDLKDWKRIGKELKQAGRKGNIIPLTVWNDWAIIKAALEPFQTEEDSISVSDAPGSGIIDCNEKTRKKSQKETESLHCEYVAEPVMAQSTQNVDYNQLQEVIYPETLKLEGKGPELVGPSESKPRGTSPLPAGQVPVTLQPQKQVKENKTQPPVAYQYWPPAELQYRPPPESQYGYPGMPPAPQGRAPYPQPPTRRLNPTAPPSRQGSELHEIIDKSRKEGDTEAWQFPVTLEPMPPGEGAQEGEPPTVEARYKSFSIKILKDMKEGVKQYGPNSPYMRTLLDSIAHGHRLIPYDWEILAKSSLSPSQFLQFKTWWIDGVQEQVRRNRAANPPVNIDADQLLGIGQNWSTISQQALMQNEAIEQVRAICLRAWEKIQDPGSTCPSFNTVRQGSKEPYPDFVARLQDVAQKSIADEKARKVIVELMAYENANPECQSAIKPLKGKVPAGSDVISEYVKACDGIGGAMHKAMLMAQAITGVVLGGQVRTFGGKCYNCGQIGHLKKNCPVLNKQNITIQATTTGREPPDLCPRCKKGKHWASQCRSKFDKNGQPLSGNEQRGQPQAPQQTGAFPIQPFVPQGFQGQQPPLSQVS",
    "response#2": "MRIYSLIDSQTLMTKGFASEVMRSPEPPKKWDIAKKKGGMRTIYHPSSKVKLIQYWLMNNVFSKLPMHNAAYAFVKNRSIKSNALLHAESKNKYYVKIDLKDFFPSIKFTDFEYAFTRYRDRIEFTTEYDKELLQLIKTICFISDSTLPIGFPTSPLIANFVARELDEKLTQKLNAIDKLNATYTRYADDIIVSTNMKGASKLILDCFKRTMKEIGPDFKINIKKFKICSASGGSIVVTGLKAEKHLRELENLVDSNLEEEKLKPHLSMGEDVQSPGKGEPLHPNVRAPLSHVVRAATIDLPRLGNKLPAKHHLGKLSGLYQMKGCSFNPEWKVPDISDTHFDLQVINECPSRNWKYLTPAKFWPKSISYFPVQAGVKAKYPDNVMQHEAIVGKYLNRLYEAGILYKRISKHLVTFKGKPYHWELQYLVKQHQVPDGTTTCKINGREENRRRRAPAKSISRPHDTERNCHMVGQVSNNRSPIRSCANNGGGKYSSTTRRMACWGGQTIGVNQSCSPRTSAASVDAGRRSKSSRGLSSLSRRETTGNHHHSTNVTNSVEAATRGRSPAGKQVSARDTSSLPESGASRACDSSPKIIYIDTGATLCFGKRKGYASYLSFIKILLKRGGVKVSTKNLIKLFQIIEQFCPWFPEQGTLDLKDWKRIGKELKQAGRKGNIIPLTVWNDWAIIKAALTK",
    "response#3": "MGQTKSKIKSKYASYLSFIKILLKRGGVKVSTKNLIKLFQIIEQFCPWFPEQGTLDLKDWKRIGKELKQAGRKGNIIPLTVWNDWAIIKAALEPFQTEEDSISVSDAPGSGIIDCNEKTRKKSQKETESLHCEYVAEPVMAQSTQNVDYNQLQEVIYPETLKLEGKGPELVGPSESKPRGTSPLPAGQVPVTLQPQKQVKENKTQPPVAYQYWPPAELQYRPPPESQYGYPGMPPAPQGRAPYPQPPTRRLNPTAPPSRQGSELHEIIDKSRKEGDTEAWQFPVTLEPMPPGEGAQEGEPPTVEARYKSFSIKILKDMKEGVKQYGPNSPYMRTLLDSIAHGHRLIPYDWEILAKSSLSPSQFLQFKTWWIDGVQEQVRRNRAANPPVNIDADQLLGIGQNWSTISQQALMQNEAIEQVRAICLRAWEKIQDPGSTCPSFNTVRQGSKEPYPDFVARLQDVAQKSIADEKARKVIVELMAYENANPECQSAIKPLKGKVPAGSDVISEYVKACDGIGGAMHKAMLMAQAITGVVLGGQVRTFGGKCYNCGQIGHLKKNCPVLNKQNITIQATTTGREPPDLCPRCKKGKHWASQCRSKFDKNGQPLSGNEQRGQPQAPQQTGAFPIQPFVPQGFQGQQPPLSQVLT"
  },
  {
    "reference": "MKVRGIQMNWPNLWNWGILIIGLVITCSTSDNLWVTVYYGVPVWKDADTTLFCASDAKAHETEVHNVWATHACVPTDPNPQEMPLTNVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTNANLTQPNATLNHTTTNSNYSIGNLTDEVKNCTFNMTTEIRDKQQKVHALFYKLDIVPMGGNGSEYRLINCNTSVIKQACPKISFDPIPIHYCAPAGYAILKCNDKKFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENITDNVKTIIVHLNKTVKINCTRPSNNTRTSIRIGPGQMFYRTGDITGDIRKAYCEINGTEWNETLNQVTKKLKQYFNKTIVFRPPSGGDLETTMHHFNCRGEFFYCNTTKLFNSTDNGTMEGFNTTITLPCRIKQIVNMWQGVGQAMYAPPISGVINCTSNITGILLTRDGGSNDNETKEIFRPGGGNIKDNWRSELYKYKVVQIEPLGIAPTKAKRRVVDREKRAVGLGAMIFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQRMLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSNKIICTTNVPWNSTWSNKSYAEIWNNMTWIEWEKEISNYTDKIYDLLTESQSQQERNEKDLLELDKWASLWNWFDITKWLWYIKIFIMIIGGLIGLRIVFAVLSIVNRVRQGYSPLSFQTPFRHQREPDRPEGIEEEGGEQGRDRSVRFVSGFLALAWDDLRSLCLFSYRRLR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Transmembrane protein gp41: Acts as a class I viral fusion protein; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain2. The protein must contain a signal peptide for proper functionality.3. The designed protein should be involved in positive regulation of receptor clustering, fusion of virus membrane with host plasma membrane, positive regulation of establishment of T cell polarity, positive regulation of plasma membrane raft polarization by localizing to virion membrane, host cell plasma membrane, plasma membrane, host cell endosome membrane, viral envelope and exhibiting structural molecule activity.4. Incorporating a YXXL motif; contains endocytosis signal motif is critical for the successful design of the protein.5. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "MRVMGIQRNCQQWWIWGILGFWMLMICNVLGNLWVTVYYGVPVWREADTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEMDLENVTEDFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLDCHNVTSSNSSS",
    "response#2": "MWVTVYYGVPVWREAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQELVLENVTENFNMWKNDMVDQMHEDVISLWDESLKPCVKLTPLCVTLNCSTILNCSTLNCSTTQSGNDTCNNNMCAEMKNCSFNTTTELRDKNRKEYALFYKLDIVPLERANNRTENNSSEEYRLINCNTSTTTQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGKGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENLTNNAKTIIVHLNESVEIVCTRPNNNTRKSIRIGPGQTFYATGAIIGDIRQAHCNISKSKWNSTLQRVGKKLAEHFPNRTIRFNSSSGGDLEITTHSFNCGGEFFYCNTSGLFNSTFNDTYAPNNTDTNETITIQCRIKQIINMWQQVGRAMYAPPIAGNITCNSSITGLLLVRDGGTKTDDNTTETFRPEGGNMRDNWRSELYKYKVVEIKPLGIAPTEAKRRVVQREKRALLGAMFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAMERYLQDQQLLGIWGCSGKLICTTAVPWNASWSNKSLDYIWGNMTWREWEKEIDNYTGIIYTLIEKSQNQQEKNEQELLELDKWASLWNWFDITKWLWYIKIFIMIIGGLIGLRIVFVILSIVNRVRQGYSPLSFQTRLPTQRGPDRPEGIEEEGGERDRDRSGQLVNGFLTIIWVDLRSLCLFSYH",
    "response#3": "MRVMGTQRNYPLLWKWGTLIFWIMTICNAEKLWVTVYYGVPVWRDADTTLFCASDAKAHVTEVHNVWATHACVPTDPNPQEIYLQNVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTNVNSTNVNTTNNRTYDGNMTEEIKNCSFNTTTEIRDKKQRAYALFYRPDIVPIRENSTEYILINCNTSTITQACPKVSFDPIPIHFCAPAGYAILKCNNKTFNGTGPCSNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIIRSENLTDNVKTIIVHLNETVEINCTRPGNNTRRSIRIGPGQTFYTHSIIGDIRQAYCNIDKGKWNKTLIQVKKKLQGYYNRTIEFKPHSGGDLEVTTHSFNCRGEFFYCSTSNLFNDNSTGDSNNITLITLPCRIKQIINMWQGVGQAMYAPPIAGTITCTSNITGLLLTRDGGNTSGTEIFRPGGGNMKDNWRSELYKYKVVEIKPLGIAPTEAKRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASVTLTVQARALLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTNVPWNNSWSNKTLEDIWDNMTWLQWDKEINNYTEIIYRLIEESQNQQEKNEQDLLALDKWASLWSWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSIINRVRQGYSPLSFQTLTHHQREPDRPEGIERGGEQGRDRSVRLVSGFLALAW"
  },
  {
    "reference": "MWRLKLGEGNGDDPYLFSSNNFVGRQTWEFDPKAGTLEERAAVEEARRSFLVNRSRVKACSDLLWRMQFLKEAKFEQVIPPVKIEDAKDITYENATDSLRRGVSFFSALQASDGHWPGEIAGPLFFLPPLVFCLYITGHLEEIFDEEHRKEMLRHVYCHQNEDGGWGLHVESKSIMFCTVLNYICLRMLGEGPNGGRDNACKRARQWILDRGGVTYIPSWGKIWLSILGIYDWSGTNPMPPEIWLLPSFVPIHLAKTLCYCRMVYMPMSYLYGKRFVGPITPLILQLREELHLQPYEAINWNKTRRLYAKEDMYFPHPLVQDLIWDTLHIFVEPLLTHWPLNKLVREKALRLAMKHIHYEDENSHYITIGCVEKVLCMLACWIDDPNGDYFKKHLARIPDYMWVAEDGMKMQSFGSQQWDTGFAVQAIIASDLSSETGDVLKRGHDYIKKSQIRENPSGDFKSMYRHISKGAWTLSDRDHGWQVSDCTAEALKCCLLLSMMPAEVVGHKMDPEQLYDSVNLLLSLQSANGGVTAWEPVRAYAWTELLNPTEFLANLVAEREYVECTSSVVQALVLFQQLYPDHKTKKISRAIEKAVQFLENEQKPDGSWYGNWGVCFIYATWFALGGLAAAGKTYKTSQAMRKGVEFLLTTQKDDGGWGESYLSCPEQRYIPLEGNRSNLVQTAWAIMGLIHAGQAERDPIPLHRAAKLIINSQMENGDFPQQEIVGVFMRNCLLHYATFRNTFPLWALAEYRKAAFVTHKH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that contributes to triterpenoid biosynthetic process by functioning in lipid droplet with beta-amyrin synthase activity.2. We need a protein that can perform (S)-2,3-epoxysqualene = beta-amyrin with high efficiency.3. For general function, the protein need meet that Component of the oleanane-type triterpene saponins biosynthetic pathway4. We require a protein that can specifically bind to the metabolites produced in the Secondary metabolite biosynthesis pathway. The designed protein sequence is ",
    "response#1": "SGFTELKFTRDRVVETYFAVASSMFEPEFSTCRAVYTKISVLLVILDDLYDGYGSPDEIKLFSEAVKRWDLSLLEQMPDHMKICFLGLYNTVNEVAEEGRKTQGHDVLGYIRNLWEIQLAAFTREAEWSQGKYVPSFDEYIENAQVSIGVATILLITILFTEEDDILSHIDYGSKFLRLASLTARLANDIKTL",
    "response#2": "AMSYFSQLQLPSGQWASECIGPLFILAFVVIAGYVTDTPLPAGYAVEIRRYLFARQCVADGGWGWHAEASESSAIGTVLSYVVLRLLGTTRDDPRLVRARTLLHEFGGATHAPGLAKFWLCILGVMKWECVNPFLPEFWLSPDSDPASPSKWYLHTRTNFTSMSYVWSKQWSYAGDAITEQLKAELYTQPYDTIDFAGHRSSLAAVDNNYPKWWLVNLMNWLTVAVYIPYFRKPV",
    "response#3": "SGFTELKFTRDRVVETYFAVASSMFEPEFSTCRAVYTKISVLLVILDDLYDGYGSPDEIKLFSEAVKRWDLSLLEQMPDHMKICFLGLYNTVNEVAEEGRKTQGHDVLGYIRNLWEIQLAAFTREAEWSQGKYVPSFDEYIENAQVSIGVATILLITILFTEEDDILSHIDYGSKFLRLASLTARLANDIKTCT"
  },
  {
    "reference": "MRILLAEDDLLLGDGIRAGLRLEGDTVEWVTDGVAAENALVTDEFDLLVLDIGLPRRSGLDILRNLRHQGLLTPVLLLTARDKVADRVAGLDSGADDYLTKPFDLDELQARVRALTRRTTGRALPQLVHGELRLDPATHQVTLSGQAVELAPREYALLRLLLENSGKVLSRNQLEQSLYGWSGDVESNAIEVHVHHLRRKLGNQLIRTVRGIGYGIDQPAP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Functions as a transcriptional activator by direct binding to a cis-acting sequence upstream of the target gene promoters including lipase lipA and pmrH promoters; Member of the two-component regulatory system PmrA/PmrB that plays a role in the regulation of resistance towards polymyxin B and cationic antimicrobial peptides in response to limiting concentrations of Mg(2+)2. The designed protein have Response regulatory domains with specific sequence motifs that are crucial for its activity.3. The protein should have DNA-binding transcription activator activity and be active in cytosol.4. The OmpR/PhoB-type domain within the designed protein should be capable of recognizing and binding to a range of DNA sequences. The designed protein sequence is ",
    "response#1": "MVGKSPAMQHLLSEIALVAPSEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQIISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIS",
    "response#2": "MVGKSPAMQHLLSEIALVAPSEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQIISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIS",
    "response#3": "MLVEDDHSISEMVDHYLTKEGFGIVHAFDGEEGIRLFQQGSYDLVLLDIMLPKLNGMDFLKIIREKSNIPVLMISAKDGDVDKALGLGFGADDYIAKPFSMIELTARVKAAAE"
  },
  {
    "reference": "MSKKYNVAVVGANGAVGEEILAILNEVNFPINKLVPLASARSAGTKIEFAGKSIIIKELTDTIFEEEEVEIALFSAGGSVSAQFAPAAVKAGAVVIDNTSHFRMDQNTPLVVPEVNPEDISKWEKTGVIANPNCSTIQMVQTLKPLDEAYDIKRIDVSTYQATSGAGKSAMEELVTQMHDFFAFKLDQSEHKAFKHQIALNVIPQIDVFMENGYTKEEMKMVNETTKIMHKQIPLSATCVRVPTLRGHAEALTITFANEVKADEARELLRKAPNIVILDNPSESLYPMPAICLEKNETFVGRIRNDNFSKNMLHLFIVADNLRVGAATNAVRIAQKWIEMEEEK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A key design requirement for the protein is the optimization of the Acyl-thioester intermediate, Proton acceptor to achieve high catalytic efficiency for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.2. A protein with aspartate-semialdehyde dehydrogenase activity, protein dimerization activity, NAD binding functions is required to enable lysine biosynthetic process via diaminopimelate, threonine biosynthetic process, 'de novo' L-methionine biosynthetic process, isoleucine biosynthetic process, diaminopimelate biosynthetic process.3. The designed protein should have a binding site that is specific to the target phosphate ligand.4. The protein design should consider the endogenous proteins and enzymes present in the L-threonine biosynthesis, L-threonine from L-aspartate: step 2/5 pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MTALVGATGAVGGVMIDIMNARQDWPWGEVRLVASPRSAGKKMTVRGEELTVLALAPEVFDGVDIAMFDVPDEVSARWAPIAAERGAVAVDNSGAFRMDPDVPLVVPEVNADQIKARAGKGIVANPNCSTMQMLVALEPIRQKYGLKRVIVSTYQAVSGAGAQAVEELYEQTRAIINNEPYEPKILPVRSDKKHYQIAFNAIPQIDKFQENGYTFEEMKMINETKKIMGLPDLQIAATCVRLPVATGHSESVYIEVEKDGLTVQECKEILAGAPGVELQDDIENQIYPMPADCVGKYEVFVGRIRQDLNKANGFHMWIVSDNLLKGAAWNSVQIAESLVKLGLVK",
    "response#2": "HIAVVGATGAVGQQIIKTLENRNFPVGKITLLSSARSAGKTALFKGEEVTIQEAKPESFEGVDIALFSAGGSVSKELAPEAVKRGAIVVDNTSAFRMDADVPLVVPEVNESALHEIPKGIVANPNCTTMAAMPVLKPLHELFKIRRVVVSTYQAVSGAGKTGMDELFTQTRGSFVHDPIEAKQFTKQIAFNVIPHIDRFLEDGSTKEEWKMAVETRKILDPDIQVLATCVRVPVFIGHSEAISIECERPIDLRKAQEALRHAPGVILRDEREDGGYVTPVECVGEDDTYVSRLRIDPTVENGLAFWCVSDNLRKGAALNAVQIAELLIKK",
    "response#3": "MSALVGATGAVGGVMIDIMNARQDWPWGEVRLVASPRSAGKKMTVRGEELTVLALAPEVFDGVDIAMFDVPDEVSARWAPIAAERGAVAVDNSGAFRMDPDVPLVVPEVNADQIDLFKTGGAKKVITAVVGATGNVGREILQILSERGVAAKAVAAVASSRSTGMEVSYGEEDVLKVDALDDFDFSGVDMVLSSPGAKVSAEFAPRAAAAGAVVIDNSSHFRMDPDVPLVVPEVNPDALDAFNKGYKDKIVNCTCVRVPVARSHLKPIYDAVGIDRINVSTYQSVSGAGKEAIEELAKQCADLLSGKPVKVENFSRQIAFNSIPQIDKFMDNGYTKEEMKMVWETKKILGDENVLVNPTAVRVPAFYGHGEAIHIETRQQTSAEEVKEILANAPGVVVAHNDEDFPTQVSDASGKDDTFVGRIREDISHPNGINMWVVADNVRGSATVIEALYKAGLVEWMSTMTYQAASGGGAQHMRELLTQFGSVYGEVKSLLDDPKSAILEIDRKVLAKQKALSADETANFGVPLGGSLIPWIDKDLGLGKSQDDAEWGMSKEEWKGGAETNKILGQGPGFDAPGIPVDGFCVRVGAMRCHSQALTFKLKKNVPLADIEAMLAQDNDWVRVVPNTREDTLKGLTPVAVTGTLQIPVGRIRKLAMGPEYVGAFTIGDQLLDGAGLEF"
  },
  {
    "reference": "MHPLPSVALLSAIGAVAAQVGPWGQCGGRSYTGETSCVSGWSCVLFNEWYSQCQPATTTSTSSVSATAAPSSTSSSKESVPSATTSKKPVPTGSSSFVKADGLKFNIDGETKYFAGTNAYWLPFLTNDADVDSVMDNLQKAGLKILRTWGFNDVNSKPSSGTVYFQLHDPSTGTTTINTGADGLQRLDYVVSAAEKRGIKLLIPLVNNWDDYGGMNAYVKAYGGSKTEWYTNSKIQSVYQAYIKAVVSRYRDSPAIMAWELSNEARCQGCSTDVIYNWTAKTSAYIKSLDPNHMVATGDEGMGVTVDSDGSYPYSTYEGSDFAKNLAAPDIDFGVFHLYTEDWGIKDNSWGNGWVTSHAKVCKAAGKPCLFEEYGLKDDHCSASLTWQKTSVSSGMAADLFWQYGQTLSTGPSPNDHFTIYYGTSDWQCGVADHLSTL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans.. The designed protein sequence is ",
    "response#1": "DKSATPEARALLKYIQLQYGSHYISGQQDIDSWNWVEKNIGVAPAILGSDFTYYSPSAVAHGGKSHAVEDVIQHAGRNGINALVWHWYAPTCLLDTAKEPWYKGFYTEATCFNVSEAVNDHGNGTNYKLLLRDIDAIAAQIKRLDQAKVPILFRPLHEPEGGWFWWGAQGPAPFKKLWDILYDRITRYHNLHNMVWVCNTADPAWYPGNDKCDIATIDHYPAVGDHGVAADQYKKLQTVTNNERVLAMAEVGPIPDPDKQARENVNWAYWMVWSGDFIEDGKQNPNQFLHKVYNDTRVL",
    "response#2": "GRFSEAVFQVLDYVIYEARRNHIRLILCLVNNLDNLGGKAQYVQWAQAAGANMTNSTDSFYSHPTIKRYYKDYVKAILTRRNSYSRIRYSDEPAIFAWELMNEPRCVSNSSGPYLQAWIAEMAAYVKSLDTNHLVTVGTEGFYGPGIAERLGVNPGEWAASLCSDFIQNSAVEHIDFASVHAYPDSWLPRASLEEKVRYLSNWVDSHLNDSEQILKKPVLFTEVGYLQHSC",
    "response#3": "DKSATPEARALLKYIQLQYGSHYISGQQDIDSWNWVEKNIGVAPAILGSDFTYYSPSAVAHGGKSHAVEDVIQHAGRNGINALVWHWYAPTCLLDTAKEPWYKGFYTEATCFNVSEAVNDHGNGTNYKLLLRDIDAIAAQIKRLDQAKVPILFRPLHEPEGGWFWWGAQGPAPFKKLWDILYDRITRYHNLHNMVWVCNTADPAWYPGNDKCDIATIDHYPAVGDHGVAADQYKKLQTVTNNERVLAMAEVGPIPDPDKQARENVNWAYWMVWSGDFIEDGKQNPNQFLHKVYNDTRVL"
  },
  {
    "reference": "MAKITKTFQYGKHTVTLETGEVARQASGAVIVKMDDTVLLVTVVAAKTAREGQDFFPLTVDYQEKFYAGGRIPGSFFKREGRATEKETLISRLIDRPIRPLFPEDYKNEVQIIAMVMSLDPEIDGDIPAMIGASAALSLAGIPFKGPIGAAKVGYNDGQYILNPTVSELKKSQLELVVAGTANAVLMVESEAALLSEEVMLGAVIFGHREMQKVIQVIDSLTAEACTQPSDWVPPAKNDALVIALKKVIGGRLSDAFHIRDKLQRRDAIAAVKDDVIQQLAGRLEVEGWNLAELLKEFGELEYRTMRDALLDTKVRIDGRSLEAVRPITVKVGVLPRVHGSGLFTRGETQAIVVTTLGTARDGQVIDAVTGEYKENFLFHYNFPPYSVGECGRFGVPKRREIGHGRLARRGVLAVMPSLDEFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPVKAPVAGIAMGLVKEGERFVVLSDILGDEDHLGDMDFKVAGTSEGVSALQMDIKIEGITEEIMRQALQQAKVGRLHILGEMDKALAAPRAELSDYAPRLLTIKIHPDKIRDVIGKGGSTIQAITKETGTQIDIQDDGTIVIASVNNAAAREAKRRIEQITSDVEPGRIYEGKVAKIMDFGAFVTILPGKDGLVHVSQISSERVERVGDKLKEGDVVRVKVLEVDKQGRIRLSIKAVEEEVASI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should contain one or more S1 motif domains that are essential for its function.2. The protein can be targeted to cytoplasm and perform polyribonucleotide nucleotidyltransferase activity.3. The designed protein should have a high specificity for Mg(2+) binding, with minimal off-target effects.4. The protein must exhibit the following characteristics: Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'- direction.; Involved in mRNA degradation. The designed protein sequence is ",
    "response#1": "ELTETVRVLCETRLKELAYTPLKKEERADQTAAIYHEIIESTIEHFKATCSSEEFSTDPAKALCINPHIIDEQIHMVEKRVMRHMILDDAKRLDGA",
    "response#2": "LEKEMLGLVEQELRAAYAIPVKQDRYAAVGKVKEKVIAHYFPEGQEPKYDKLRIAGVFKELEAKIVRWNILDTGKRIDGRK",
    "response#3": "ELSEYVKNACESSLKELAYKPLAKEVRADETKAIYSETVKKAIEHFSAAFGPEALAADPSKALCLNEHIIEEEIHSFEKNVMRRMILDDGKRLDGRH"
  },
  {
    "reference": "MPSGQPVFPRRVPDAYIAMRGLVVASLLILLVGGTDAFRICAFNAHRLTLTKVIKESVMDTLVQILARCDIMVLQEVVDSSQNTVLFLLQKLQSSKSYSFLNSSLLGRSTYKEKYVYIYRSDKTQVLNFYQYNDTEDIFAREPFVAQFTLPSKILPSVVLVPLHTTPKDVEKELNALYDVFLDVSQRWQNENVILLGDFNADCASLAKKRLNSLLLRTKAGFHWVIPDGEDTTVRASTNCTYDRIVMHGQGCQKLLKAAATFDFPRRFQLTEEEALRVSDHYPVEVELNKAAQGIPPHCLSTLLLLSLSQLG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Include a signal peptide as an essential component of the protein.2. The active site active site of the designed protein must be optimized for substrate binding and specificity.3. A protein that localizes to endoplasmic reticulum and can perform DNA binding, deoxyribonuclease I activity with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "SGAFIVNGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGHLLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEC",
    "response#2": "SGAFIVNGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGHLLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGED",
    "response#3": "PYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGHLLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTC"
  },
  {
    "reference": "MAGHLGPDFAFSPPPGGGEPWVDPRGWYGYPAPPGAAGAGAGAGAGGEPWGGPFLPPPAGPLYEGGGWAGGCFVPQIGVGLAQPPPDPSGPDGGRGGRRDAPPGRLPRAGGLRAGPEAGAGRERRRASPPRPRPQQETPSREELEQFAKELKRKRITLGYTQADVGVTLGALFGKVFSQTTICRFEAQQLSFKNMCKLRPLLQRWLEAADDNDRLQEMCNAETVLQQARKRKRTSIENKVRGNLETMFLQCPKPNLQQISSIAEELGLEKDVVRVWFCNRRQKGKRGSGCSSREDFETAGPFPSGPVHLPLPPGPSLGPPGYGGPPFATLYSPAPFHDGDAFAPVPIAPLGPPTHSS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein's functionality will benefit from Pro residues compositional bias in its structure.2. The designed protein should be involved in positive regulation of transcription initiation by RNA polymerase II, cellular response to leukemia inhibitory factor by localizing to female germ cell nucleus, male germ cell nucleus and exhibiting RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding.3. A protein with Homeobox capability for targeted gene regulation. The designed protein sequence is ",
    "response#1": "QSGKWRRASRERRIGNSLEKFFQRCPKPTPQQISHIAGCLQLQKDVVRVWFYNRSKMGSRC",
    "response#2": "LKRDKDQIYGHPLFPLLALVFEKCELATCTPREPGVAGGDVCSSDSFNEDIAVFAKQIRAEKPLFSSNPELDNLMIQSIQVLRFHLLELEKVHELCDNFCHRYISCLKGKMPIK",
    "response#3": "LKRDKDQIYGHPLFPLLALVFEKCELATCTPREPGVAGGDVCSSDSFNEDIAVFAKQIRAEKPLFSSNPELDNLMIQSIQVLRFHLLELEKVHELCDNFCHRYISCLKGKMPIC"
  },
  {
    "reference": "MTTRTDLRNLTQEEIVDYVESLGQPAFRGRQIMAWLYRPGISEFSQMTDLAKDFRRILSEHAYMSSFQNPLIERSKDGCVKFGFRLDDGKMIESVLIPEPDRNTLCVSSQVGCAMNCSFCLTATMGFIRNLSPAEIVNQVCAVGDFLRAESKGNLIGSDRVTNIVFMGMGEPLNNLDNLLKSLSILTEQRGLDLTNRRITVSTCGIVPQMQILGENSTANLAVSLHAVDDETRDKLMPINRRYPVAELIEACRTFPMAKRKRIMFEYILLKGINDSDAEAHKLAKLLRGIPCKINLLPYNESPAIPYKSPSYSRILSFQKILMNAKYSVFVRSSRGSDISAACGQLAREENSDIS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein's S-methylcysteine intermediate, Proton acceptor must be carefully engineered to ensure that the amino acids involved in adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine are properly positioned for optimal catalysis.2. The designed protein should be involved in rRNA base methylation by localizing to cytoplasm and exhibiting 4 iron, 4 sulfur cluster binding, tRNA binding, rRNA (adenine-C2-)-methyltransferase activity, tRNA (adenine-C2-)-methyltransferase activity.3. The designed protein must have at least one Radical SAM core domain.4. For general function, the protein need meet that m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs5. The designed protein should have a high specificity for [4Fe-4S] cluster binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MDGRTDILGLPKAQIAELFREAGLDAKQAKLRAKQVWHWIYHRGVSAFEDMTDIAKTMRPWLTERFVIARPEVVEAHHSSDGTRKWLLRTADSHDFEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNLTPGEIVGQVMLARDALGEWPKGRMDVGEVEDESEYRADGRLLTNIVMMGMGEPLYNFDNVRDALKIVMDAEGLALSRRRITLSTSGVVPMMDRCGREIGVNLAVSLHAVNKETRDEIVPLNRKYGIDELLQACADYHGASNARRITFEYVMLKDKNDSDEDARELVRLIRKYDLPAKVNLIPFNPWDGSAYECSDPARIRAFSNIVFEAGISAPVRTPRGRDIDAACGQLKTAAERKSRRTRRLA",
    "response#2": "MLSRINLLDLDRAGLESFVAGLGEKPFRARQLQRWIHRSLAADFDAMTDLAKSFRAKLPELAEIRAPQPIRDHLSADGTRKWLFDVGNGDAVESVFIPEDDRGTLCVSTQAGCAVNCRFCSTGKQGFSRNLAVGEIIGQLWYAERELRRSLGTPEGERVISNVVLMGMGEPLLNYEASVAAMRLMLDDDAYGLSRRRVTLSTSGVVPFIDRLGTDLPVALAVSLHASNDKLRDVLVPLNVKYPLAELMEACHRYLAHAPRDFITFEYVMLDGVNDRVEYARELLALVREHGVSCKFNLIPFNPFPESGLGRTPMERIKAFAGVLIDAGIVTTIRKTRGDDIDAACGQLAGDVRDRTRLGLP",
    "response#3": "LAGMTRNQIRDCLIATGLVEEKKAKMRSGQIWRWVYQYGITDFAAMTDISKELRERLPEHFSLTKLQVVHQETSSDGTMKFLFELHDGASIETVLMREDDRGTLCVSTQAGCAVNCRFCSTGKQGFSRNLAVGEIIGQLWYAERELRRSLGTPEGERVISNVVLMGMGEPLLNYEASVAAMRLMLDDDAYGLSRRRVTLSTSGVVPFIDRLGTDLPVALAVSLHASNDKLRDVLVPLNVKYPLAELMEACHRYLAHAPRDFITFEYVMLDGVNDRVEYARELLALVREHGVSCKFNLIPFNPFPESGLGRTPMERIKAFAGVLIDAGIVTTIRKTRGDDIDAACGQLAGQVADRTRRSARYRSRG"
  },
  {
    "reference": "MQNERRIAQRFLEDKQLPYSSNELITMALTHPSYAQEKNTVANNQRLEFLGDAVLNLVVAEYLYNHYGRKAEGELTKIRAKVVCEDALAIFARNINLGQYLLLGRGEEMSGGRKRKSILADAVEAVIGAIYLDQGLETVQRFIIRQLEELISETASGDYYDYKSKLQELVQARDKENVSYAIIEESGPAHAKVFVAGVYYREQLLATGEGKSKKEAEQKAAQKVLQDNLVPGT",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be able to bind Mg(2+) ligand in a variety of conditions2. The protein with the reaction Endonucleolytic cleavage to 5'-phosphomonoester., the active site must be optimized to ensure efficient catalysis.3. The protein must bind to Mg(2+) in order to perform its enzymatic function.4. The protein must exhibit the following characteristics: Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.; Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon; Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S).5. The designed protein must have at least one RNase III domain.6. The ideal protein would be one that plays a critical role in tRNA processing through metal ion binding, ribonuclease III activity, rRNA binding within cytoplasm. The designed protein sequence is ",
    "response#1": "MDGVDELLLRKFNLTLPPGLLRTAFVHKSFSFENGACQNNERLEFLGDAVLGLVVSHYLFETCPEYTEGQLSAARSYIVSGTSIAQIAKELNLGQFMLLGKGEKLAGGMDKTSLLADLLESLIGAVMVDQLDILNRQGLDYKTALQELTQAKFGETPEYVILGSSGPDHKKEFEVAVCVLGSEYARACGSSKKEAQQEAARIALEIFHQ",
    "response#2": "MAAIEQRLGHTFADISLLTTAFTHVSALKSSRGRCDSYQRLEFLGDHVLGLVVSDMLYRAFPKADEGELSKRLADLVRKETCIDVAKSLDLQEGVKLGAVGAGAGARLRRSVLGDICEAVIGAVYLDGGFRAACELLDRYFADTRAQLERVNHKSVLQQFAQRELSATPVYKLIRETGPDHRKMFLMGAMVDDRRFAPAWGNNKKDAEQRAAANALAELHLEGLLRTAFVHKSFSFENGACQNNERLEFLGDAVLGLVVSHYLFETCPEYTEGQLSAARSYIVSGTSIAQIAKELNLGQFMLLGKGEKLAGGMDKTSLLADLLESLIGAVMVDQGFESARDFVLREFGFPNDHRAAVAMMEKSVLQQFAQRELSATPVYKLIRETGPDHRKMFLMGAMVDDRRFAPAWGNNKKDAEQRAAANALAEG",
    "response#3": "MDGVDELLLRKFNLTLPPGLLRTAFVHKSFSFENGACQNNERLEFLGDAVLGLVVSHYLFETCPEYTEGQLSAARSYIVSGTSIAQIAKELNLGQFMLLGKGEKLAGGMDKTSLLADLLESLIGAVMVDQLHNEARLRKQTDYKTALQELTQAKFGETPEYVILGSSGPDHKKEFEVAVCVLGSEYARACGSSKKEAQQEAARIALEIFHS"
  },
  {
    "reference": "MLSPISNLKIDFESRPLIFKKPAQTSRDTLIKKPCYYLRATTIDGRIAIGECSLIPGLSRETEGEAVAELERLVGGDTLDLNDIETASVRFAAEMILNELHDELPETGFSRGAEGMDINGLVWMGSGEEMLAQVEELKSKGFKTIKLKIGALDFEDELQILKRTREICPYPEYALRLDANGAFGEDVLEKLEQLSHFDIHSLEQPIAAGQTSLMHRICQKSPIPIALDEELIGINDESEMAQLLDAIKPQFIILKPSLLGGLDVANKWIDLAEARGISWWSTSALESNIGLQAIADWTAHKAATSNLCVGVSGLGTGSLYTNNTPATLKISEGKIWHVPKITVDQKHYPLTAEGAQLLEQHGPNWTSGIADFLEFWFNTDAPLTCSTSGSTGEPKLITHSRQAATHSAQQTLTYFDLTPGDSAVLALPIDFIAGKMMLIRAIVGDLNLIAINPSISSPLPKAQFAALTPHQALKAHVSLNNIDKLLLGGSPTPTSLITEGLDVYEGFGMTETITHIALRKHGEERFTALPGIELSTEAGRLIINSPQRGVHSILTDDLAEVKGNEFIWLGRHSDTINSGGLKFNPEKIEEKLRTVIDEDLAVYGVDHEEYGQCVALRIDISQPSTEELEELIGKVESVVSDKERPRVFKFGLIHRNTAGKILRKEMANDLPVIVFATGNPNKAKEVARLLEGKYIVKSLPDIGCNEDIPETADTLEGNAALKARYVKENYGYDCFADDTGLEVKALNGAPGVYTARYGGPEKNADANM",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.2. The designed protein must be able to perform XTP diphosphatase activity under specific conditions. The designed protein sequence is ",
    "response#1": "TVLLGVTGSIAAYKALDLAGRLRRRGINVVAAMTGAACRLVGPASFESITGNPVARELFPENKPQTIEHIALAELADAVAVVPATANFLAKAAHGLADDLLTAAVLATKSPKLAAPAMNVGMWENPVVQGNLKKLKAGGWQVVEPEEGFLACGTRGAGRLARVEIIEQAILG",
    "response#2": "TVLLGVTGSIAAYKALDLAGRLRRRGINVVAAMTGAACRLVGPASFESITGNPVARELFPENKPQTIEHIALAELADAVAVVPATANFLAKAAHGLADDLLTAAVLATKSPKLAAPAMNVGMWENPVVQGNLKKLKAGGWQVVEPEEGFLACGTRGAGRLARVEIIEQAILAEL",
    "response#3": "TVLLGVTGSIAAYKALDLAGRLRRRGINVVAAMTGAACRLVGPASFESITGNPVARELFPENKPQTIEHIALAELADAVAVVPATANFLAKAAHGLADDLLTAAVLATKSPKLAAPAMNVGMWENPVVQGNLKKLKAGGWQVVEPEEGFLACGTRGAGRLARVEIIEQAILSRV"
  },
  {
    "reference": "MQSNVIKIIIGASLLIGVGALYLLLSLKTPDKPLAGQVNIYEDNAEIGGDFELIDQNGELFNSDELKGKLSLIYFGFTSCPDICPTSLNKITKAVEILSENKIDIVPVFITIDPSRDTPAVLKEYLKHFHPKFIGLTGDEKQIREVADKFKVYYAKAASENDNDQDYMLDHSSFTYLMDKNGKYLKHFYLDSAPSKIMEFLKSN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Cu cation binding. The designed protein sequence is ",
    "response#1": "SCETTVTSGDTMTYSTRSISVPASCAEFTVNFEHKGHMPKTGMGHNWVLAKSADVGDVAKEGAHAGADNNFVTPGDKRVIAFTPIIGGGEKTSVKFKVSALSKDEAYTYFCSYPGHFSMMRGTLKLF",
    "response#2": "RLPSWIESGLSEMRLEKDKFSINLDVKHFSPEELKVKVSGDFIEIHGKHEERQDEHGYVSRDFQRRYKIPVDVDPLSITSSLSPDGVLTVCGPRKQGDVPERSIPITRL",
    "response#3": "YFTLDFPTTPEKAYDFVNGAPNDIANDTQAANGTRAIVFEYGSRIQIIFQNTGTLTTENHPIHLHGHSFYVIGYGTGNYDQQTAKFNLEDPPYLNTIGVPVGGWAAIRFVANNPGLWLLHCHFDIHQTWGMSTMFIVKNP"
  },
  {
    "reference": "MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLKLTVFESDKWSFEAETETVAGIPAGTDNLIYQVAKRTADLYGKEMPPVHVKVWSDIPLARGLGSSAAAIVAAIELADELCGLKLSEADKLHLASLEEGHPDNAGASLVGGLVIGLHEDDETQMIRVPNADIDVVVVIPFYEVLTRDARDVLPKEFPYADAVKASAVSNILIAAIMSKDWPLVGKIMKKDMFHQPYRAMLVPELSKVEHVAEMKGAYGTALSGAGPTILVMTEKGKGEELKEQLALHFPHCEVDALTVPKEGSIIERNPLYQVKSV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have ATP binding to facilitate phosphorylation, threonine biosynthetic process.2. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate..3. A protein that can modulate the overall flux of metabolites through the L-threonine from L-aspartate: step 4/5 pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "LQGIASGITNTNYFVATGTGRYVLTLFEEHSAEELPNFLDLMTHLAERGIPCPYPVKNIAGQALGELNGKPAALVSCLAGKSLDNPSPQHCAAIGEVLARMHLAGASFEVSIINLRCQRWRIARLPRWAPFLDAENXRMLDAQLEFEQAFDTSALSRGVIHADLFRDNVLMDGDKVGGVLDFYYAWNDVLFYDIAIAVXDWCVNADGTRDVGRVRAFLDAYHAVRPG",
    "response#2": "LQGIASGITNTNYFVATGTGRYVLTLFEEHSAEELPNFLDLMTHLAERGIPCPYPVKNIAGQALGELNGKPAALVSCLAGKSLDNPSPQHCAAIGEVLARMHLAGASFEVSIINLRCQRWRIARLPRWAPFLDAENXRMLDAQLEFEQAFDTSALSRGVIHADLFRDNVLMDGDKVGGVLDFYYAWNDVLFYDIAIAVXDWCVNADGTRDVGRVRAFLDAYHAVRPG",
    "response#3": "PVHKFGGSSLADAAGFHRVANIINAQQADALIVVSASGKTTNRLIELLNLFISGDDAAAIVLEGIYSYQQQLICNVLSDDVAAPLLAELERDISDIASLLEHQKLDLYQRNKVQSYGEVWSARLLAHLLNSQGRGATPIDARQFLQVSGTPVKVEHAYSSAHLNALLNQYQGQCLVITGFIAADGQGQTRLLGRNGSDFTATLVAELAGSSHTTIWSDVPGVFSADPRRVTAAQLLERMSLAEANELARLGSPVLHNRTLGPVADSQQQLMS"
  },
  {
    "reference": "MNPNIETAYALAKQQYAAQGVDVDAAIAALDNIPVSMHCWQGDDVTGFEASAGALTGGIQATGNYPGKARNAQELRQDLELAMTLIPGAKRLNLHAIYLESEQPVARDQIQPEHFANWVKWAKKMNIGLDFNPSCFSHPLSADGLTLSHPDAKIRQFWIDHCKASRRISAYFGKELGTASVMNIWIPDGMKDLPADRLAPRQRLLAALDEVISEKFDEAHHIDAVESKLFGIGAESYTVGSNEFYMGYAASRNVALCLDAGHFHPTEVISDKISAASLYVKHLLLHVSRPVRWDSDHVVLLDDETQAIATEIIRNDLLDRVHIGLDFFDASINRIAAWVIGTRNMKKALLRALLEPTQALRDAEAKWDFSSRLALMEETKSMPWQAVWDYVCARNGVAVGADWLATVKNYETEVLATRK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have a specific function in rhamnose catabolic process and should be localized to cytoplasm in order to carry out this function efficiently.2. We require a protein that can specifically bind to the metabolites produced in the glycerone phosphate from L-rhamnose: step 1/3, L-rhamnose degradation pathway.3. For general function, the protein need meet that Catalyzes the interconversion of L-rhamnose and L-rhamnulose.4. The Mn(2+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "KEMGFIGGKMALHHGPAEGEEGLRKNLDILNDMRNKVGKDFWLMQDCWMALDVEYATRFSTIAWNEMGLKWTEEALPPDDYWGYAALKKNAPKGHLITTGEHEATRWGFKMLLDMECCDIIQPDVGWCGGITELIKITALADAAGVLVVPHGSSVYSYHFVITRHNSPFAEFLMMHPVPDQVVPMFTPLLLDEPVPVNGRLKLDDTPGFGVRLNPL",
    "response#2": "AYLSSGTWSLMGFESLTPYTCDAALQANITNEGGAEGRYRVLKNIMGLWLLQRVLKEQNVSDLQGLIARTAALPACRFIIDCNDDRFINPASMSAEIQAACRDAGQPVPESDAELARCIFDSLALLYARVLNELAALRGHPFSQLHIVGGGCQNTLLNQLCADACGIVVVAGPIEASTLGNIGIQLMAYLSSGTWSLMGFESGTPLTHQQAQCSNITNEGGAEGRYRVLKNIMGLWLLQRATDELQIDDLPQLIEQAARQPACRSLINPNDSRFINPPNMCREIQNACREHQFPVPNTAAQLARCIFDSLAMLYRQVAQELATLRGRPISHLHIVGGGCQNQFLNQLCADACGLNVSMGPVEASTLGNIGSQLSAADYIEQFQSLS",
    "response#3": "KEMGFIGGKMALHHGPAEGEEGLRKNLDILNDMRNKVGKDFWLMQDCWMALDVEYATRFSTIAWNEMGLKWTEEALPPDDYWGYAALKKNAPKGHLITTGEHEATRWGFKMLLDMECCDIIQPDVGWCGGITELIKITALADAAGVLVVPHGSSVYSYHFVITRHNSPFAEFLMMHPVPDQVVPMFTPLLLDEPVPVNGRLKLDDTPGFGVRLNPY"
  },
  {
    "reference": "MPTGQSFAAIVVAAGSGIRAGQAVPKQFAPWRGKPVLRYSVEALLDAGAAPVVVVIPENGDALTLRALEGLTDIHLVTGGATRQASVLAGLEALTDHKPGMVLIHDAARPDLPASVINRLLEALDEESGAIPVLPVVDSMVEASGSVMGAPADRDILRRVQTPQAFRFSDILQAHRAWQGEPDAGDDAQVLRAAGHTIALVDGDERLHKLTFAADFAPEQAKVALPAPRIGMGYDVHRLAAGEELWLCGLRIEHTHGLAGHSDADVGLHAVVDAVLGAAGRGDIGQHFPPSDPQWRGASSDRFVEHAVSLAAQAGYRVGNVDLTLICEAPKIGPHRDAMQAKLADLLGVDTSQVNLKATTTERLGFAGRREGIAAQAVAMLLPI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must have at least one 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domain.2. The designed protein should be able to compete with endogenous proteins or inhibitors for 4-CDP-2-C-methyl-D-erythritol 2-phosphate binding.3. The protein must have high specificity towards metal ion binding, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity.4. The protein should be optimized for activity in isopentenyl diphosphate biosynthesis via DXP pathway pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "VKITTPADLALLSSPMNEAKPPRTGWGYDVHRYLASNETDAKTKSQARPMMLGGIAIPGGPQVIAHSDGDVLLHALTDALLGVACAGDIGQLFPDTDQAFANMPSAVFVTEALRRVRACGYRPAHADLTVVAQVPKLGPHKARMAAQIAQLLELPQACVNVKATTEEGLGFTGEKRGIKAIAVVL",
    "response#2": "IRLGNGYDVHAFGPGDHVWLCGVQIPHDAGLVGHSDADVGMHALTDAIYGALAEGDIGRHFPPSDPQWKGADSAIFLRHAAELARKRGFAFGNADITLICEAPKIGPHAGAMQDRLAAIMDIDASRISVKATTSERLGFTGRREGIAAIATIALITV",
    "response#3": "TGWGYDVHKYGPGRPMVLGGVPIQGAPEVVAHSDGDVLLHALADALLGCLGRGDIGQHFPDTDAAFEGIESSILVNEILLMADKDGLNITHVDLTLICQKPRLAPHTAQIKSSVERLLRLNSKQVNVKATTEEGLGFTGKKLGIKAVACVI"
  },
  {
    "reference": "MKIVIATGNPGKFREIAKLLADDPVELLSLADFPHLPEVVEDGATFAANAEKKALSVARATGLPTLADDSGLTVAALGGAPGVRSARYAGEDADDAANNRRLLQELEGVPEAERTAAFVCDLAFCLPDGRCSHFTGRLEGRILTEARGAGGFGYDPLFYVEGYGCTLAEMPLDRKNAISHRGQALARFVEALPELLTHL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in dITP + H2O = dIMP + diphosphate + H(+) are properly positioned for optimal catalysis.2. The protein must bind to Mg(2+) in order to perform its enzymatic function.3. The protein should have a high affinity for substrate.4. The designed protein must possess nucleotide binding, metal ion binding, dITP diphosphatase activity to support nucleotide metabolic process, purine nucleoside triphosphate catabolic process under certain conditions. The designed protein sequence is ",
    "response#1": "MIKVIIASNNAKKLRELQA",
    "response#2": "MHKLVIATRNAKKAVELEALFAEVG",
    "response#3": "MTLILALRR"
  },
  {
    "reference": "MQMDILLNGIDIIKIEGEVDHITGVTDNSRETSTGYLFAALRGEKVDGHKFVNEAILNGAAILLVEEDLSENVPNIKVPNTRKAFAKIVSNYYGNPSSEMKIIGVTGTNGKTTTSLLLSSIYRDSICFSTIKYITSDGEKKSINTTPAPLILQKELHSALNKGIKTAIIEVSSHGIVQDRIFSIDFDYGVFTNISRDHLDYHKTFEAYREAKSRFFKSLHRDKMALINVDDANAPYFIKNTDSKVITYGFKKGNIKGQIIENTFYNLILKIEGMDRSLTVRSPLIGTYNAYNILASASVAIIDDVEDSRIIEGIQEVYSPQGRMEKVNVPVPFSVIVDYAHTPDALNNAISSLRDLNCNRVITVFGAGGERDKGKRAIMGMITSKLSDIVIVTSDNPRSEDPEAIIDDIFEGIEDEEVYRIEDRKEAIFKALDIARKGDFVLIAGKGHEEYQEIKGQRIPFSDRECVKEYFK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC",
    "response#2": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC",
    "response#3": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC"
  },
  {
    "reference": "MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRAFLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQKSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKSLPANVVCRGNPAVVIRERVETE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that can modulate the overall flux of metabolites through the Slime biogenesis, slime polysaccharide biosynthesis pathway, enhancing or suppressing its activity as needed.2. The designed protein should be optimized for O-acyltransferase activity, O-acetyltransferase activity activity in order to improve colanic acid biosynthetic process, slime layer polysaccharide biosynthetic process, lipopolysaccharide biosynthetic process, single-species biofilm formation. The designed protein sequence is ",
    "response#1": "MPATKFSRRTLLTAGSALAVLPFLRALPVQR",
    "response#2": "MPATKFSRRTLLTAGSALAVLPFLRALPVQQ",
    "response#3": "QTLFETLGEKHQFNGTVLAAEGGDILYHHSFGYAEMTEKRPLKTNSLFELASLSKPFTALGIILLEEKGILGYEDKVDRWLPGFPYQGVTIRHLLNHTSGLPDYMGWFFANWDSHKIAVNQDIVDMLMNEGLSGYFEPNEGWMYSNTGYVLLAVIIEKASGMSYADFIKTSIFLPAGMNETRVYNRRLSPERIDHYAYGYVYDVHSETYVLPDELEETNYVVYLDGIQGDGTVNSVTSDLFRFDQALYQDDFISKASKESAFSPVRLNNGETIDYGFGWVLQNSPEKGRIVSHSGGWPGYSTMMIRYIDHRKTLIYLSNKEEDTEYEQWL"
  },
  {
    "reference": "SGGEMSNMGHSMEE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must be able to alpha-amylase inhibitor activity. The designed protein sequence is ",
    "response#1": "CYPGQAFQVPALPACRPLLRLQCNGSQVPEAVLRDCCQQLAHISEWCRCGALYSMLDSMYKEHGAQEGQAGTGAFPRCRREVVKLTAASITAVCRLPIVVDASGDGAYVC",
    "response#2": "CAPGLAIPAPPLPTCRTFARPRTCGLGGPYGPVDPSPVLKQRCCRELAAVPSRCRCAALGFMMDGVDAPLQDFRGCTREMQRIYAVSRLTRAAECNLPTIPGGGC",
    "response#3": "CYPGQAFQVPALPACRPLLRLQCNGSQVPEAVLRDCCQQLAHISEWCRCGALYSMLDSMYKEHGAQEGQAGTGAFPRCRREVVKLTAASITAVCRLPIVVDASGDGAYVV"
  },
  {
    "reference": "MSESTTANPILHLPRLPESNGAPAPVAAAVGSGANPYLEMDLLRFSTAGSVDDGKSTLIGRLLYDTKSIFEDQLEAIEGTSRKRGEGYVNLALLTDGLRAEREQNITIDVAYRYFATPKRKFIIADTPGHIQYTRNMVTGASTAELAIVLVDARKGVLTQSKRHGFLASLLGIPHMVVAVNKMDLVGYSQDVYNRIVADYTLFAQKLEVKDLIFIPISALHGDNIVNHSERMPWYDGATLLHHLENVKIGADLNLVDFRYPVQYVIRPHQDFRGFAGRVVSGTITPGEEVVVLPSGQGSRVRSVERFDGAVEEASPLDSVVLTLEDEIDVSRGDMIVRRMNLPTVGNRFDAILCWMNETPLDVRTPYVMMHTTRCVKAFVSNLVYRIDVDTLHREQVETFGLNDIGRVEMTAAQPIFFDGYQRNQATGSFVLIDPHGHVTVAAGIIRGEVRTADELFKDEEAERAPAATAVSPDVVWEEWNVAREEREARNGHRAAVLWFTGLSGAGKSTIARELERALFAAGCQTMLLDGDQLRHGLCGDLGFGEADRRENIRRVGEVARLFFQQGGLVLCTFVSPFPEDRERVRALLPEGSFFEIYVRCSLEECMRRDPKGLYAKAVAGKIESFTGVSSPYEPPEHSEIIVDTDRESVDTIVESILAELDRAGILPQAAGA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that is capable of catalyzing reactions within the hydrogen sulfide biosynthesis, sulfite from sulfate: step 2/3, Sulfur metabolism pathway with high efficiency and specificity.2. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.3. The Phosphoserine intermediate of the protein must be engineered to enable effective catalysis of adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate   + ADP + H(+).4. A protein with ATP binding, sulfate adenylyltransferase (ATP) activity, adenylylsulfate kinase activity functions is required to enable phosphorylation, sulfate assimilation.5. The protein must exhibit the following characteristics: With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway; APS synthesis involves the formation of a high-energy phosphoric- sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD..6. The designed protein have Tr-type G domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MLRFITCGSVDDGKSTLIGRLLWDSKLVFEDQLAALEVDSKKVGTQGEEIDYALLLDGLQAEREQGITIDVAYRFFSTDKRKFIVADTPGHEQYTRNMVTGASTAGVAVILVDARKGVLTQTRRHSFLVSLVGIRKIILAINKMDLVDYDAERYETICDEYREFAKDLGFEEIACIPISALKGDNVIERTDVTSWYDGPMRFPVQWVNRPNADFRGFSGTLAEGTSKPGDQVQVLPSGQIACIERILLGHRELPQLAKGQAATLTLDRALDVSRGDIIVAPGDAPPAEASDQFEATIVWMDEAALKPGRGYWLKLGSQMVTATVQPPKHEVDVNSFEHLAAKTLELNSIGVAEIATDRPITFEPYESSRQLGGFILIDKFTNATVGAGMLHFALRRSQNIHWQAVDVDRAAHAAQKGQQPLCLWFTGLSGSGKSTIANLVEKRLHALGKHTYLLDGDNVRHGLNKDLGFTDADRVENIRRVTEVAKLMVDAGLIVLTAFISPFRAERRLVRDLMRAGEFVEIFVDVPLEVAEERDPKGLYKKARSGQLPNMTGINSPYEAPEAPEIRVNTVEMEAERAAERIVDHLLRFMTCGSVDDGKSTLIGRLLFEAQVLFSDQVEALKEESKRQGTQAGAIDFALLVDGLAAEREQGITIDVAYRFFSTARRKFIVADTPGHEQYTRNMATGASTADLAVILVDAAQGLLDQTRRHSFIASLLGVRHVVLAVNKMDRVDYDSAVFEEILAQYHSFAKRLDFTDVTAIPVSALCGDNVS",
    "response#2": "MLRFITCGSVDDGKSTLIGRLLWDSKLVFEDQLAALEVDSKKVGTQGEEIDYALLLDGLQAEREQGITIDVAYRFFSTDKRKFIVADTPGHEQYTRNMVTGASTAGVAVILVDARKGVLTQTRRHSFLVSLVGIRKIILAINKMDLVDYDAERYETICDEYREFAKDLGFEEIACIPISALKGDNVIERTDVTSWYDGPLRFPVQLVSRPDRTFRGYSGQLSSGILRKGTEIMALPSMQKSRVKSIVTFDGELEEARPGQAITITLEDERDISRGDMLVHPTDRPSEAADQFSATIVWMDEAPLLPGRGYILKLGTQTAGVTVQAPDHVVDINTLAKLSARSLGLNDIGVADIHTDRPIVFGPYASGAALGGFIIIDRATNATVATGMVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMLHQALRRADNIHWQSLDVDRDARARQKHQTPKVLWFTGLSGAGKSTIANLVEKRLHALGAHTFLLDGDNVRHGLNKDLGFTDADRVENIRRVGEVAKLMVDAGLIVLTAFISPFRAEREMVRAMLPEGEFVEVYVDTPLEVAEERDVKGLYKKARSGELKNFTGIDSPYEAPERPEIRVDTTAMSAEQAAERVVAGLLPGL",
    "response#3": "MLRFITCGSVDDGKSTLIGRLLWDSKLVFEDQLAALEVDSKKVGTQGEEIDYALLLDGLQAEREQGITIDVAYRFFSTDKRKFIVADTPGHEQYTRNMVTGASTAGVAVILVDARKGVLTQTRRHSFLVSLVGIRKIILAINKMDLVDYDAERYETICDEYREFAKDLGFEEIACIPISALKGDNVIERTDVTSWYDGPADQFETTLVWMHDEPLLPGRPYLLKIGAKTVTATVTHIKYQVNVNTLEHVAARTLELNAIGVCNLSLDRPIAFDPYAENRDTGGFILIDRLSNNTVGAGLVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMVAQPAAEGGGKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGM"
  },
  {
    "reference": "MLLNDREIKELIDKKEIIIEPFSYEQIQPASIDLRLGNRFRIFRKGEIEVIDPKDFKDELIKIEQDENKIIEKYKYTDVIITENPFIIYPGDFVLASIYEYIKLPRYIAAQLHGKSSIARLGLIIHTSAGWIDPGYEGHLTLEIFNTNNVPIKLYPKMKIAQLQLFRINPVERDYKEKGGKYYKEKGATSSEIWKDFI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The Proton donor/acceptor of the protein must be engineered to enable effective catalysis of dCTP + 2 H2O = diphosphate + dUMP + NH4(+).2. The protein design must be able to perform nucleotide binding, dCTP deaminase activity in order to contribute to dUMP biosynthetic process.3. Ideal protein should have a binding affinity for key enzymes in the dUMP biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "QLPHYATPGAAGLDLRACIDAPITLAPGETRLVPTGMAIHLADPGYAALILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMVSAWNRSEQSFELVPLERLAQLVIVPVVQARFNIVEAFETTARGEGGFGSTG",
    "response#2": "AGMDLYSIEEVVIPPGETKLIKTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFKVEKHMKIAQMIL",
    "response#3": "PPAYASAGAAGLDLRACLDAPVLLHPGETTLVPSGLAIHLADPGLAAMVLPRSGLGHKHGIVLGNLVGLIDSDYQGQVFVSVWNRGRDVFTIQPMERIAQLVVVPVLQVGFNVVDDFAASERGEGGFGSTG"
  },
  {
    "reference": "MTTTIDTNQWCSRFVKCKGCKLDAECMVKPEEMALVREDGKIVDKWAIRTTEMIARELEKLKAI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Ral interferes with the cleavage of DNA by E.coli EcoK restriction-modification system, by modifying the activity of the host methylase 2. The protein design should aim to mimic or enhance the function of natural proteins involved in evasion by virus of host restriction-modification system, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "PIYLALRRMELPEAQLNRWLLAYWCLYNGGEASYLSEFEGREFFEMLNHAAENVREAPIGGRWPRGAERRHWRGAQATSSVEYLIDRYDDRPEDMAAYCAGQGGTFLEVTKRVQEHRLFGPWIGFKVADMVDRVLGKPVSFDNAAVFMFKDPYKAACIQYEVNPNIPDHVLADGSVAPRNRELVTPETVHHVAQHLIEHFKGFQAPPLGDRPVNIQEVETILCKWKSHQNS",
    "response#2": "PIYLALRRMELPEAQLNRWLLAYWCLYNGGEASYLSEFEGREFFEMLNHAAENVREAPIGGRWPRGAERRHWRGAQATSSVEYLIDRYDDRPEDMAAYCAGQGGTFLEVTKRVQEHRLFGPWIGFKVADMVDRVLGKPVSFDNAAVFMFKDPYKAACIQYEVNPNIPDHVLADGSVAPRNRELVTPETVHHVAQHLIEHFKGFQAPPLGDRPVNIQEVETILCKWKSHQNL",
    "response#3": "PIYLALRRMELPEAQLNRWLLAYWCLYNGGEASYLSEFEGREFFEMLNHAAENVREAPIGGRWPRGAERRHWRGAQATSSVEYLIDRYDDRPEDMAAYCAGQGGTFLEVTKRVQEHRLFGPWIGFKVADMVDRVLGKPVSFDNAAVFMFKDPYKAACIQYEVNPNIPDHVLADGSVAPRNRELVTPETVHHVAQHLIEHFKGFQAPPLGDRPVNIQEVETILCKWKSHQNK"
  },
  {
    "reference": "MSSKLHMCRWMLLVIGVCCLMGSSSGSFCSLERMKKFAMEACEHLFQADEGARRDRRSIEFAHHHLNRLGSGKTHNKHHYISRSSYPMGGYLKVTREHFNRLSELDIFPRYKPIKPHHEKKHRFKRDHSSRSYNNIPYCCLNQCEEEFFC",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be optimized for G protein-coupled receptor binding, receptor ligand activity activity in order to improve chorion-containing eggshell formation. The designed protein sequence is ",
    "response#1": "GNVAVLTVILSIRPESTPVPRFLMCHLAFADLCLGLYLLLVACIDAHSMGEYFNFAYDWQYGLGCKVAGFLTVFASHLSVFTLTVITIERWLAITQAMYLNHRIKLRPAALIMLGGWIYSMLMSSLPLFGISNYSSTSICLPMENRDVYDTIYLIAILGSNGVAFSIIAVCYAQIYLSLGRETRQAHQNSPGELSVAKKMALLVFTNFACWSPIAFFGLTALAGYPLINVTKSKILLVFFYPLNSCADPYLYAVLSKQYRNAFRRLLCGKSTA",
    "response#2": "GNIGMILLIKTDSRLQTPMYFFPQHLAFVDICYTSAITPKMLQSFTEENNLITFRGCVIQFLVYATFATSDCYLLAIMAMDCYVAICKPLRYPMIMSQTVYIQLVAGSYIIGSINASVHTGFTFSLSFCKSNKINHFFCDGLPILALSCSNIDINIILDVVFVGFDLMFTELVIIFSYIYIMVTILKMSSTAGRKKSFSTCASHLTAVTIFYGTLSYMYLQPQSNNSQENMKVASIFYGTVIPMLNPLISALNY",
    "response#3": "GNVTVLFVLLTSHYKLTVPRFLMCNLSFADFCMGLYLLLIASVDSQTKGQYYNHAIDWQTGSGCSAAGFFTVFASELSVYTLTVITLERWHTITYAIQLDQKLRLRHAIPIMLGGWLFSTLIAMLPLVGVSNYRKVSICLPMDIETTLSQVYILTILILNVVAFFIICACYIKIYFAVQNPQLIATNKDTKIAKKMAVLIFTDFTCMAPISFFAISAAFKVPLITVTNSKVLLVLFYPVNSCANPFLYALLSKQFRNALRKIIGC"
  },
  {
    "reference": "MEANFNPDKQHTVGAEISISGTGLHTGVKVDMTFRPAAPGFGIQFQRIDLAGQPIIKADCDLVTDTSRGTTIEDNGARVSTVEHVLAALVGMGVDNVLIEINGPETPIMDGSSQPFVEMLQEVGVQEQEAAKAWYSIDENIYHYDEKKRVEMVAMPSMDYQITTLIDFNSPVLGTQHAGLKNMREFVKEISPCRTFVFLHELEMLLDNNLIKGGDINNAIVVVDKPVTSDEMQRLAKAFGRDKMEVKHEGYLNNLELRFPNEPARHKLLDVVGDLALIGYPVKARIIANRPGHSTNVEFAKKIKQYIKKNRHLKDVPVYDCNQPPIYTAQQIEKTLPHRYPFLLVDKIIELTDKVIVGVKNVTFNEPFFTGHFPGNPVMPGVLLCEALAQTGGILAINSMPAGTYDTYFLKIDNCKFKQKIVPGDTMLLKMELSAPIRRGICEMRGTVYVGNKLCAEADMVAQVVKRG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that can modulate the overall flux of metabolites through the lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 2/6, lipid IV(A) biosynthesis, Glycolipid biosynthesis pathway, enhancing or suppressing its activity as needed.2. The designed protein should be involved in fatty acid biosynthetic process, lipid A biosynthetic process by localizing to cytoplasm and exhibiting 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity, 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity, metal ion binding.3. For general function, the protein need meet that Involved in unsaturated fatty acids biosynthesis4. The designed protein must have a active site active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "MVDNKNSNPTKPKTAVPVYWWTGRTTKELKSFPLESNNPILRDFAEKKGLHYLDVRTVMPDAQKNPDLVLFKAPKDAYCVVIFDGSRTALLTEKEFADSKMYGCLVADEGREYKGVVKYTPDTHFYVYDWKTGKEKAVAATDPKEMLAKYKANPKLKLPTDPKAKLVADEHKHKPGHVCGAYLPQDIYYDAGGDYYCWGCDSKFDLAKFKNDPRWKKIVEEKKKVIDEYTK",
    "response#2": "MTDSATFTDIQDLPALPGVYVWFGAEGEVLYVGKAKNLRTRVSSYFQPQLTSPKTEAMVQAEFVVCSSEAEALLLENRLIKRHMPRYNVRLRDDKTYPYLAVSHEPEFPRLTLTRRVVQDGARYFGPYPNGYAVRETLDLLQKIFPLRTCSDTVFENRTRPCLLHQIGRCKGPCVGLVSAEEYAEQVDQVRLFLQGKNVADVMAQLETAMEAAAERQDFETAARYRDRIRAVTQVQARQFVSDNQDGDADIVGLATSGDAACLQLFFVRGGKNIGRDHSFPLKGLQGDEAALLTAFIQEFYAEVPRPPKEVLLGLDLPDQEGLAAMLSTLADEVPVTLPRRGQKRELVTLARQAANEELQRASRSASQTRAALDVLQGLKEALGLERMPQMIA",
    "response#3": "MSNVAFGSDNVVVAGSFYEPAERDTIAYGIKQAGENWFKPIGVGFDWQADDARIASLGEAGALCRKLSTKHQRVVRDLYIIHGKAEASQVTAIEQEIAAQVLADLHATQGFVIATRSGDALDVAIHRLGHPLLAHVGVVEVKSGERLVWTPEWGKNWSPQARDQALQVCDALVAAINATREGWS"
  },
  {
    "reference": "MDRKRLLKNPLLWILVALLLYFGISTLADSDRGFTEIPTSQAIEQIRSGNVSEATLEDREQQLKLQLGDSIGVEGQQVEQVITKFPAGASDQLYNQLLNAGTEQQDVRFNTTVTQESFFSQLLIYMIPLGLLVLLLMWMMNSAQGGGNRVLNFGKSKAKQLNKDMPTTTFTDVAGANEAVEELHEIKDFLQSPARYQALGAKIPKGVLLYGPPGTGKTLLARAVAGEAGVPFYTISGSDFVEMFVGVGASRVRDLFEQAKQNAPCIIFVDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFDSRGGIILIAATNRPDILDPALLRPGRFDRQIPVSAPDLRGRKAILEVHSKGKPLAENSDLDALAKRTVGMSGADLANVINEAALLTARQHGSVITDAALEESVDRVIGGPARKSRIISEHERKITAYHEGGHALAAWAMPDIEPVYKLTILPRGRTGGHALVVPEDDKQLMTRSEMIGRLVFAMGGRAAEELVFHEPTTGASSDIEQATKIAKAMVTEYGMSPRLGAVKYGQEHGDPFLGRSAGRQPDYSLEVAHEIDEEVRKLIETAHTEAWEVLTAYRDVLDDLVMEVLEKETLQRRDLERVFESVEKRPRITTFNEFGERLPSSKPPIKTPRELALERGEPWPPDAEDEDEATTSPESPESPESPEPSDAPEPEPTPVGTAPGGNDLPGGPPHTSPEPPATSSTYNPYAPPSGPSGDRPSNGSPRTNGGAKSWTPDRGGPDSGGTSNGPPHYGAPPGWTP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein localizes to the plasma membrane.2. The protein should be able to bind ATP ligand in a variety of conditions3. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "FSKELGDGGFGTVYYGTLKDGRAVAVKRLFERSLKRVEQFKNEIDILKSLKHPNLVILYGCTTRHSRELLLVYEYISNGTLAEHLHGNQAQSRPICWPARLQIAIETASALSYLHASGIIHRDVKTTNILLDSNYQVKVADFGLSRLFPMDQTHISTAPQGTPGYVDPEYYQCYRLNEKSDVYSFGVVLSELISSKEAVDITRHRHDINLANMAISKIQNDAVHELADLSLGFARDPSVKKMMSSVAELAFRCLQQERDVRPSMDEIVEVL",
    "response#2": "FNPDNQLGEGGFGRVYKGQIETPEQVVAVKQLDRNGYQGNREFLVEVMMLSLLHHQNLVNLVGYCADGDQRILVYEYMQNGSLEDHLLELARNKKKPLDWDTRMKVAAGAARGLEYLHETADPPVIYRDFKASNILLDEEFNPKLSDFGLAKVGPTGGETHVSTRVMGTYGYCAPEYALTGQLTVKSDVYSFGVVFLEMITGRRVIDTTKPTEEQNLVTWASPLFKDRRKFTLMADPLLEGKYPIKGLYQALAVAAMCLQEEAATRPMMSDVVTALEYIHA",
    "response#3": "LEALVGKGRFAEVWRARLSHNEGGQYETVAVKIFPAVEYSSWCNERAIFSDANLKHENIVQFLTAEERGGTAASPQRQYWLIMAYYNMGNLQEFLAGHILTWTELCSLAGSVARGLAHLHSDTTPCGIPKVPIAHRDLKSSNIVLKNHSECALCDFGLALRLDLSLTVDDFANSGQVGTARYMAPEVLESRVNLEDLESFKQIDIYSMALVLWEMF"
  },
  {
    "reference": "MASYTLKPLRVVRSIQQIRHYSAPLDNAIPASKQKYVPTSGTYPKGFLVSGIHVGVKPANTRFPDLALITSETPCSGAAVFTKNKFQAAPVTVSREMLQQRNGKGVRAIVINSGCANAVTGKGGLEDAESMARTVDACFADPEAPASDTPAISSTIVMSTGVIGQRLPIQKILSQIPTAHSSLASTHSGWLTAARAICTTDTFPKLLSRSFTLPSSPHTTYHLAGMTKGAGMIHPNMATLLGILCTDAPIPPSTLSPLLTHAVSHSFNAISIDGDTSTNDTVAILANGAAGGPEIASSTSPDGLAMQETLTKFAQDLAHLVVRDGEGATKFVHIHVVNALSVAAARAIASSVATSPLVKTALYGRDANWGRILCAVGYTPGVEAGVIVPEKTSVSLVPVDGSEELKLLVEGEPEAVDEERAAGILESEDLVIRVDLGEGRRGGEGGGELVDL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a high specificity for substrate binding, with minimal off-target effects.2. We require a protein that can specifically bind to the metabolites produced in the Amino-acid biosynthesis, N(2)-acetyl- L-ornithine from L-glutamate: step 1/4, L-arginine biosynthesis pathway.3. A protein with methione N-acyltransferase activity, glutamate N-acetyltransferase activity, acetyl-CoA:L-glutamate N-acetyltransferase activity is needed to enhance arginine biosynthetic process in a specific context.4. The protein with the reaction acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate, the Nucleophile must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MSNVRFGTDGWRAVIAEEFTFENVRVVAQAIAEKLGGPTKPVMLGYDTRFLSERFALAAARVLSANGVEVLLSERTTPTPVVSFTVRDLGIAGGINITASHNPPEYNGIKFNPEYGGPAPPEITALVEARIAEAETVPPSRVPFDEALERSALVSVDPLEPYVSHILSLVDMEAIRAAGLKVVFDAMYGAGSGYMPSLLEPLGIEVQAIHNQLDPNFPGMPEPNMEHLRELVKAVRQHDVDVGLALDGDADRVGVIDSTGAFFTPNHILAILIEHLLTERPGGRGAIVRSVATSHMLDRVAAKHGVELYEVPVGFKWIAQRMREEDILMGGEESGGIALRGHLPERDGILSGLMLAEMTAVSGKSVSELLSDLERRFGRHYYARQDVRLPENRDRGAELRRALTAAPTQTLAGARVLSVDEVDGIKFYLEDGSWVLLRASGTEPKLKVYAEAFGATRAEVDALVERYRTLVESV",
    "response#2": "MSNQVFNKVILASGNQGKIKEFAQLLAPLGVEVVPQSAFNVSEAEEPHVTFVENALAKARHASQLTGLPALADDSGLCVEALGGAPGIYSARFAGEPKSDARNNAKLLAELAGVADRQAYFHCTLVYMRHAQDPLPIIAQGQWQGQIIDEPRGANGFGYDPLFYLPQLGCTAAELDAQVKNQISHRGQALTQLVERLSALV",
    "response#3": "MLKGKVVLATHNAGKIREIGALLAPYGIECISAGELGLPEPAETGTTFVENALIKARAACAASGLPALADDSGLSVAALDGRPGVYTADWAERHWFEGEPGRDWYMAMGKVEGLLAAKGASDRAAAFVCALALAWPDGHVETFEGRVDGELTWPPRGTLGFGYDPVFVPTGSDLTFGEMAAEEKHGWKPGQPALSHRARAFVKFVASCLE"
  },
  {
    "reference": "MSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMATDYPSYLVADINPDLINLYKKIAADCEAFISRARVLFEIANREVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRKFAEKAQRATFICASFDETLAMLQVGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEYRSSEGHPVIVSNSDTSLIRSLYRNFTHHYIKAKRSIGVSAGESKSATEIIAVSGARCWVGFDPSRGVDSSAVYEVRV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: An alpha subtype methylase that recognizes the double- stranded sequence 5'-GATC-3' and methylates A-2 on both strands; May play a regulatory role in the functions of the retron.. The designed protein sequence is ",
    "response#1": "TVEGKWTKATLSIDELQLIKRVIAHPDVRKFKDIAKVDVGRYCDGANNYFLVDNETVKLYKLERFAHPMFGRSQHCPGIIYDEKQHIENQEKGLPTNFLYIDEEFEYLSKSVKNYIKLGEVEEYHKRYKCRIRKPWFKVPSVYSTEIGMLKRCHDAPRLIHNRVRAYTTDTAYRVSSTVTSTENLVCSFLNPITVITAELEGLFYGGGVLELVPSEIEKLYILIVEGLEHNVEELNLLIKDGQIERVIRQQGSLILGTLGFTQEENEKLVEIGRSLEIEGYVY",
    "response#2": "TVEGKWTKATLSIDELQLIKRVIAHPDVRKFKDIAKVDVGRYCDGANNYFLVDNETVKLYKLERFAHPMFGRSQHCPGIIYDEKQHIENQEKGLPTNFLYIDEEFEYLSKSVKNYIKLGEVEEYHKRYKCRIRKPWFKVPSVYSTEIGMLKRCHDAPRLIHNRVRAYTTDTAYRVSSTVTSTENLVCSFLNPITVITAELEGLFYGGGVLELVPSEIEKLYILIVEGLEHNVEELNLLIKDGQIERVIRQQGSLILGTLGFTQEENEKLVEIGRSLEIEGYVQ",
    "response#3": "TVEGKWTKATLSIDELQLIKRVIAHPDVRKFKDIAKVDVGRYCDGANNYFLVDNETVKLYKLERFAHPMFGRSQHCPGIIYDEKQHIENQEKGLPTNFLYIDEEFEYLSKSVKNYIKLGEVEEYHKRYKCRIRKPWFKVPSVYSTEIGMLKRCHDAPRLIHNRVRAYTTDTAYRVSSTVTSTENLVCSFLNPITVITAELEGLFYGGGVLELVPSEIEKLYILIVEGLEHNVEELNLLIKDGQIERVIRQQGSLILGTLGFTQEENEKLVEIGRSLEIEGYVL"
  },
  {
    "reference": "MDRLTVVVGITGGIAAYKAVGVVRLLVKAGHDVHVVPTEAALKFVGLPTLEALSRNPVTASLYDDVAAVRHVALGQSADLVIVAPATANTLAKLAAGLADDLLGTTILATKAPVLLAPAMHTEMWANAATQANVATLRSRGYAVVGPDSGALTGADSGPGRMSEPEAIVEAALALVGEGAGAQDLANLHVLVSAGGTREPLDPVRFLGNRSSGKQGVALANAALARGARVTLVAAHLEVPPPAPHPRLSVVDVSTAESLSDAMRSAATEADIVVMAAAVADYRPENVESGKIKKSGGGPLLLRLVENPDILAGLARDKRPGQVVVGFAAETAASRDELLDLGRAKAARKGADLLALNAVGWSQGFGTDENAVVLVTGAGDVVAEAAGTKADVADALLTAALGVRGAPA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.2. The designed protein should contain one or more DNA/pantothenate metabolism flavoprotein C-terminal domains that are essential for its function.3. A protein that can modulate the overall flux of metabolites through the Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed.4. The protein design must be able to perform phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity in order to contribute to coenzyme A biosynthetic process. The designed protein sequence is ",
    "response#1": "MKIALGVSGGIAAYKAAEIVRLLQDRGIRVQVVMTAAAQEFVRPLTFAALSGEKVITSMFGSGGDEQANVESAIEHIAIAQAIDALVVAPATADVLARFAQGIANDFLSTLYLATTAPVVVAPAMNVNMWNHVATQANLEILRKRGVRIVEPGSGYLACGMTGAGRLAEDETIVAAVMEHLLEQPGALPLKGKRVLITCGATREYIDPVRFISNDSSGEMGFSLARVARWLGAEVKVIAGFTTAKEPPEVYIERVKTAEDMLNAVLKEFENADVVIMNSAVADYRPKEFKDKKIKKKETLTLELVKTPDILEDLGRRKKGQFLVGFALETDNILQNAKEKLRRKNLDMIVANPSSAMGSENYKIFSQGGLTETLPMMSKYQLAHRLLDEIKKLV",
    "response#2": "MKIALGVSGGIAAYKAAEIVRLLQDRGIRVQVVMTAAAQEFVRPLTFAALSGEKVITSMFGSGGDEQANVESAIEHIAIAQAIDALVVAPATADVLARFAQGIANDFLSTLYLATTAPVVVAPAMNVNMWNHVATQANLEILRKRGVRIVEPGSGYLACGMTGAGRLAEDETIVAAVMEAAARVLAPKDLKGKRVLITCGATREYIDPVRFISNDSSGEMGFSLARVARWLGAEVKVIAGFTTAKEPPEVYIERVKTAEDMLNAVLKEFENADVVIMNSAVADYRPKEFKDKKIKKKETLTLELVKTPDILEDLGRRKKGQFLVGFALETDNILQNAKEKLRRKNLDMIVANPSSAMGSENYKIVSKTGIEDWPAMSKDDVAQKLVERVAQHFSSSP",
    "response#3": "MKIALGVSGGIAAYKAAEIVRLLQDRGIRVQVVMTAAAQEFVRPLTFAALSGEKVITSMFGSGGDEQANVESAIEHIAIAQAIDALVVAPATADVLARFAQGIANDFLSTLYLATTAPVVVAPAMNVNMWNHVATQANLEILRKRGVRIVEPGSGYLACGMTGAGRLAEDETIVAAVMEAVEVALSARAGRNIIVTAGPTRENIDGVRFISNHSSGKMGFAIACAAARRGADVTLITGPVALETPADVRRIDVTTACEMDQAVKDISGACDIFIGAAAVADYRPAEPVDGKIRKTAETMQLSLVRNPDILAGFASRKQPGQLAIGFALETEGNLDAAREKLQAKRLDLIAFNTYDGMTTGFDVDTNILTLITASGVEQWPRLSKAEVGARLADLIAERWSG"
  },
  {
    "reference": "MGSPLGSTHSGAAVVAAPSPDWVVTAATMQAIETAMFEAGMPVAALMEKAAQRLAAWVMAEYPSVAYPRVAVLVGPGHNGGDALVVAREMAHRGYSVQVLAPTDRHKSLTADHLRYVCALGVPIAPGLSDDAPSQAVDLWIDGLFGFGLERSLEGSLADLVAVVNAHPAPVVSLDLPSGLHTDTGNVLGVAVRATHTLCLGLWKRAFCQDVALPYLGHRHRIDFDIPPFALAAGLNNLPPVRHISTSDALARLPLPRAANCHKYQVSHLLIVAGSRQYAGAALLAGLGARASGVGMLTLAVPESLRWMVVAQLPEALVIGCPETDAGAIAQFPTELALNRYDAITCGPGLSRQAGAVVDAVCATDRPLLLDADALNHLAERDPIATLAQRPAPTLITPHLGEFRRLFPDWSAQPSEQSLDSGQLAQAAAAHSQSIVVLKGACTAIAHPDGRLWFNADSTPGLARGGSGDVLTGLMGGLLAQAFAPSSLSQRQGDPPPPSHTLDLALDAALAGVWWHSQTAKDMATRHTELGVDGPRLAAALTATLAARSPTLKL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must have high specificity towards metal ion binding.2. The protein should be designed to catalyze the following reaction (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.3. The protein should have a high affinity for K(+).4. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.5. The protein requires Mg(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "VLVIGGDYGMAGAAALAAEAALRCGAGLVRVATRPQHVAALVARTPEIMPVGVESGADLQPLLDSADTLVVGPGLGQSPWSEYLLQAALASGKPMVLDADGLNLLAAGKVQPRPRMIVTPHPGEAARLLGVSTAEVQADRFAATSALQQKLGGVVVLKGNGSLIASEKEMLLSDYGNPGMASGGMGDVLSGVIGALLAQGQEPLAAAALGVCLHGAAADIAAEEGMRGLVATDLV",
    "response#2": "MYQADRLAMKSGVSGILLMHEAGQAVARAVCDIIPEGRVLVLCGPGNNGGDGFVAAAALDELGYEVQLYSMLPLPELKGEALHFAKEWRGTVARELDAENFAQADVIVDALFGAGLARPLDRNCCDFVGWSRKKRVVSVDVPSGVSGDTGEVFGDVAFQATKTVTFFMKKTGHCLYPGADLCGDLVVADIGIPKEIAE",
    "response#3": "MRQVDRFAIEDIGMREEVLMENAGRAWVEQLEKLYAGLCKKKIAVLVGKGNNGGDGFVIARTLLDRGFHVDVWLLAAEEQWKGAAKYHKRLFESCGYEGKNWDVLAFMEKAYDLCVDAMLGTGATGALRYPYSEAADCLNKSGSIITAVDIPSGVPAGEGDVAEEAVQADATITLQSAKCSAYLFPARSYYGDITAVDIGIPQSAWLPQRALNANKGDAGSAGLLGGSPSMTGALILAARSALHSGAGRVYAAALGNAQSGAPMLDPLQPEIMFRDPASLREAPLTALGVGPGLGRSDVSRAVLASALDFEGPLVLDADALVLLPTLLDAVQQRHEPTLLTPHPGEAAALLCTDIPSIEANRIQAACDIAAKYHATVVLKGCGSIIATPDGRWWINTTGNPGMATAGMGDVLTGITTALLAQGMAPEQALCAAVWLHGAAADVCVER"
  },
  {
    "reference": "MFRILYLFVLCGAVSASIFNFMHDQYQHQEPRHEVSFEQRVLESNCDKYLCPGTLECVARPQDCPCPYPSSQMRCVLPNGDPLCISKPTGDYNGKYDDPAKNWKVDAKDDNVRDCGWVKRAWEGRV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Probable component of the endoplasmic reticulum-associated degradation (ERAD) pathway.. The designed protein sequence is ",
    "response#1": "MP",
    "response#2": "MGQ",
    "response#3": "MSS"
  },
  {
    "reference": "MNIVTKAAPQAIRLSDQTLCSSPLYVTYLIHYYRMSVSLSEVNECIKMGRENALLGNYDTSLVYYQGLQQQIQRLISSIKDPTRKRKWMEARDQINQEYEQVKEINETLASFRSNNPRAVNANADQDYSSPFGDYSRHEEPTRDPDVWPPPTPVEHRPSPNIRGGVRPGPRKAEPVRSRNQPAPSKPGAPGGDRRRATPGYGNVDRGREKPGEKGREKKKPEDGEKKFDGQGYDKDLVEGLERDIVQKNPNIRWDQVADLVEAKKLLQEAVVLPLIIPDFFKGIRRPWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSATLTSKYRGESEKLVKLLFEMARFYAPSTIFIDEIDSICSKRGSDSEHEASRRVKAELLVQMDGMYKYIPLPTIIYIYSYPKVHTPTHKYIYIPLPKAEGREELLKINLREVKLADDVNLTAIAQELTGYSGADITNVCRDAAMMSFRRRITGLTPEQVRQIPKEELQLPPTMEDFKEAIKKVNKSVSQADLEKYENWMNEFGSV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The ideal protein would be one that plays a critical role in microtubule severing, cell cycle through isomerase activity, microtubule binding within microtubule, spindle pole, cytoplasm, centrosome.2. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.3. For general function, the protein need meet that Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner; Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation.4. The protein should be designed to have high stability and activity towards n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1)   alpha/beta tubulin heterodimers..5. The designed protein should contain one or more AAA+ ATPase domains that are essential for its function. The designed protein sequence is ",
    "response#1": "VCMVGPPGTGKTLLAKAVATECKTTFFNVTSSTLTSKYRGDSEKLVRLLFAMARAFAPSTIFIDEIDSICSRRGTESEHEASRRVKSELLIQMDGIAGDGADPTKGVLVLAATNFPWDLDEALRRRLEKRVLIYDYTLEQVIKEEDIADLEEAKKLLREAVVLPMWMPDFFKGIRRPWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPTTIFIDEIDSICSRRGTSDEHEASRRVKSELLVQMDGVGGALENDDPSKMVMVLAATNFPWDIDEALRRRLEKRIYL",
    "response#2": "MAHHLREARQSALYGNYDGSLTFYEGVMMEIEQLIQLNYEDPKLKINKLNLIKYRKMIEAEHDQVKEYQEAWPAKGLLLFGPPGNGKTLLARAVATECSATFFSISAATLTSKYVGDGEKLVRALFAVAREMQPSIIFIDEVDSLLSERSSGEHEATRRLKTEFLVQFDGLPANSESDKIVVMAATNRPQELDEAALRRFPKRVYVTLPGLEERERLVQLYFDKYTLAEDIRLEDIADKIEGYSGADITNVCRDASLMAMRRRISGLSPEEVRALSFDKEELQMPVTMEDFNLTLKKISKSVSVADLEKYEAWMSEFGSA",
    "response#3": "DIADLTEAKRLLEEAVVLPMWMPDFFKGIRRPWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPSTIFIDEIDSLCSRRGSDSEHEASRRVKSELLVQMDGLGSATDEPAKVVMVLAATNFPWDIDEALRRRLEKRIYIPLPSTKDADMKRLAILTEGYSGSDLTALAKDAALEPIRELKSKEELQMPVTMEDFNLTLKKISKSVSVADLEKYEAWMSEFGSV"
  },
  {
    "reference": "MEIKPNEKIRVRLESFNHELLNTSCQKIMEITQNNNVDNVGVVSLPTDKRIYCVLRSPHVNKDSREHFEIRTHKRILEISYDSSVNIFDLLVKSDLPPGVLYRICLS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The ideal protein would be one that plays a critical role in translation through structural constituent of ribosome, tRNA binding within mitochondrion, small ribosomal subunit, chloroplast.2. The protein must exhibit the following characteristics: Involved in the binding of tRNA to the ribosomes.. The designed protein sequence is ",
    "response#1": "MAPGACMPLGKVPLGTTVHNVELKPGAGGQLVRSAGSSARVIARDGNYTLVTLPSGEKRLILSTCHATIGAVSNADRKNTKLGKAGRSRWLGRRPSVRGVAMNPVDHPMGGGEGKTSGGRHPVSPWGFG",
    "response#2": "MAPGACMPLGKVPLGTTVHNVELKPGAGGQLVRSAGSSARVIARDGNYTLVTLPSGEKRLILSTCHATIGAVSNADRKNTKLGKAGRSRWLGRRPSVRGVAMNPVDHPMGGGEGKTSGGRHPVSPWGFK",
    "response#3": "MAPGACMPLGKVPLGTTVHNVELKPGAGGQLVRSAGSSARVIARDGNYTLVTLPSGEKRLILSTCHATIGAVSNADRKNTKLGKAGRSRWLGRRPSVRGVAMNPVDHPMGGGEGKTSGGRHPVSPWGFR"
  },
  {
    "reference": "MARGPRYKVPKRRRREGKTNYYKRYKMVLSGHPRFVVRKTLKHIIVQIVTAKPEGDITIAAAHSRELYKKYGWMGGLGNTPAAYLTGLLAALRGLKAGIKYAVPDIGLHVPTRGAKVFAAIKAANDVGLKVPVGEEVVPSDDRIRGEHIASWAKMLQEASPEAYERFFSKYISRGFDPTQLPTHFEEVKNRILEEYKDVLGE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit 5S rRNA binding. The designed protein sequence is ",
    "response#1": "EDTDFQITVGKITQGLVKRCMADPKFYPQSSFVQLVQTNTLSYSLCPDLLSLFLEKRDVPLLQLCLHSFPDVPEVILCSCLKAFLSISEKLVNAAQINTELASLYIDVGDKDKEHKYTEHPEEPSVLQNGFSPTALEEDSCDELIAESLPQTTQKATCPISIKRAVLVNSILISPYNESFLLPHLKDMSGDQVMFFLRYLLYLYLKFNENITINHPGKQMPTVSQIVDWMSMLLDAHFATVVMLSDAKALLNKIQKTVKSQLKFYSEMNKIEGCLAELKELKCPARVSARYSIEVLQLC",
    "response#2": "EDTDFQITVGKITQGLVKRCMADPKFYPQSSFVQLVQTNTLSYSLCPDLLSLFLEKRDVPLLQLCLHSFPDVPEVILCSCLKAFLSISEKLVNAAQINTELASLYIDVGDKDKEHKYTEHPEEPSVLQNGFSPTALEEDSCDELIAESLPQTTQKATCPISIKRAVLVNSILISPYNESFLLPHLKDMSGDQVMFFLRYLLYLYLKFNENITINHPGKQMPTVSQIVDWMSMLLDAHFATVVMLSDAKALLNKIQKTVKSQLKFYSEMNKIEGCLAELKELKCPARVSARYSIEVLQLC",
    "response#3": "EDTDFQITVGKITQGLVKRCMADPKFYPQSSFVQLVQTNTLSYSLCPDLLSLFLEKRDVPLLQLCLHSFPDVPEVILCSCLKAFLSISEKLVNAAQINTELASLYIDVGDKDKEHKYTEHPEEPSVLQNGFSPTALEEDSCDELIAESLPQTTQKATCPISIKRAVLVNSILISPYNESFLLPHLKDMSGDQVMFFLRYLLYLYLKFNENITINHPGKQMPTVSQIVDWMSMLLDAHFATVVMLSDAKALLNKIQKTVKSQLKFYSEMNKIEGCLAELKELKCPARVSARYSIEVLQLQ"
  },
  {
    "reference": "MAAVTAALVKELRERTGLGMMECKKALVEAEGDIERAIDDLRKSGQAKAAKKAGRTAAEGAIAVAVSGDGKTAIMVEINSETDFVARDDNFLGFANKVAEAALAAAKTEAADIAGVELADGSTVEQAREALIQKIGENIQVRRAAILSAESALGAYVHGGKIGVLVALKGGDEALGRDVAMHVAAVAPQVVNPSEVPESDLEREKEIIRAQPDMAGKPAEIVEKMLGGRIQKFLKEISLVEQPFVKD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECR",
    "response#2": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECR",
    "response#3": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECR"
  },
  {
    "reference": "MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASAPH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in hemolysis in another organism, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "NWNVQCWVTGDLKLLICAVEPSRQNPCKNYDLHLFYALLEVAGGSRLAPQKGSFEIVQCNCSSEDRWECRVPVPAAKLHDSFLMYLKITAAWATFWSPLMSVQPS",
    "response#2": "NWNVQCWVTGDLKLLICAVEPSRQNPCKNYDLHLFYALLEVAGGSRLAPQKGSFEIVQCNCSSEDRWECRVPVPAAKLHDSFLMYLKITAAWATFWSPLMSVQPR",
    "response#3": "PLPPSKVKAEITGNVGLLKVSWETPSFSENQLQFQIRYGLSGQEIPWKTHEVDDAMIMSTSVPVRDLCAAYAVQVRCRRLDGLN"
  },
  {
    "reference": "MTLAEEAAELDAADPLAAKRAEFDLDPAVAYFDGNSLGAPPRHVAQRLADVVREQWGGRLIRSWTDGWWDAPERVGMRIAPLVGAAPGQVVVADSTSVDLFKVLVAAVRANPGRSEILLDAATFPTDGYVTEQVGGLTGHAVRRVPVAELPAACGEATAAVLVNHVDYVSGRLHDMGAITAAAHAAGAAVVWDLCHSVGAIEVRLDEHEVDYAVGCTYKFLNGGPGAPAFVYVASRLQESFDQPLAGWAGHADPFAMEAGYRAGAGISRARAGTPDILSLLALDAALDVWDDVDLAVLRAKGLALGDFFLRCLDVLLPGADVPTPRDHARGHQISVRVPDAPRTMAALHERGVLGDFRPPDVLRFGLAPLYARYEEVLRAVTALHELS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that contributes to quinolinate biosynthetic process, anthranilate metabolic process, L-kynurenine catabolic process, 'de novo' NAD biosynthetic process from tryptophan by functioning in cytoplasm with pyridoxal phosphate binding.2. A protein that can modulate the overall flux of metabolites through the Cofactor biosynthesis, quinolinate from L-kynurenine: step 2/3 pathway, enhancing or suppressing its activity as needed.3. The designed protein should contain one or more Aminotransferase class V domains that are essential for its function.4. The protein requires pyridoxal 5'-phosphate to activate a critical catalytic site.5. The desired protein should be able to perform H2O + L-kynurenine = anthranilate + H(+) + L-alanine6. The designed protein should have a binding site that is specific to the target pyridoxal 5'-phosphate ligand. The designed protein sequence is ",
    "response#1": "MTQQPKSAREYMSRQMDNWENLAVEGWFEGATPWMFYHKELKALMANIVGASAAEVCPMNTLTVNLHLLMVSFYRPTAKRFKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVAPRAGEATLRTEDILDTIATNGAETALVLFGGINYFTGQWYDMPAITKAGHHAGAVVGFDLAHAAGNVPLQLHNWDIDFACWCSYKYQNGGPGGISGIFVHEKHFAQPKSAREYMSRQMDNWENLAVEGWFEGATPWMFYHKELKALMANIVGASAAEVCPMNTLTVNLHLLMVSFYRPTAKRFKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVAPRAGEATLRTEDILDTIATNGAETALVLFGGINYFTGQWYDMPAITKAGHHAGAVVGFDLAHAAGNVPLQLHNWDIDFACWCSYKYQNGGPGGISGIFVHEKHFAY",
    "response#2": "QPKSAREYMSRQMDNWENLAVEGWFEGATPWMFYHKELKALMANIVGASAAEVCPMNTLTVNLHLLMVSFYRPTAKRFKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVAPRAGEATLRTEDILDTIATNGAETALVLFGGINYFTGQWYDMPAITKAGHHAGAVVGFDLAHAAGNVPLQLHNWDIDFACWCSYKYQNGGPGGISGIFVHEKHFAGIKGPTIGQPKSAREYMSRQMDNWENLAVEGWFEGATPWMFYHKELKALMANIVGASAAEVCPMNTLTVNLHLLMVSFYRPTAKRFKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVAPRAGEATLRTEDILDTIATNGAETALVLFGGINYFTGQWYDMPAITKAGHHAGAVVGFDLAHAAGNVPLQLHNWDIDFACWCSYKYQNGGPGGISGIFVHEKHFAGVKGPTIGPTNGPTNGPTNGPTNGPTNGPTNGPTNGPTNGPTNGPTNGPTNGPTNGPTNGPTNGPTNGPTNGPTNGPTNGPTNRLTSDQTNGLSSDQTNGLSSDQTNGLSSDQTNGLSSDQTNGLSSDQTNGLSSDQTNGLSSDQTNGLSSDQTNGLSSDQTNGLSSDQTNGLSSDQTNGLSSDQTNGLSSDQTNGLSSDQTNGLSSDQTNGLSSDQTNGLSSDQTNGLSSDQ",
    "response#3": "MQPKSAREYMSRQMDNWENLAVEGWFEGATPWMFYHKELKALMANIVGASAAEVCPMNTLTVNLHLLMVSFYRPTAKRFKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVAPRAGEATLRTEDILDTIATNGAETALVLFGGINYFTGQWYDMPAITKAGHHAGAVVGFDLAHAAGNVPLQLHNWDIDFACWCSYKYQNGGPGGISGIFVHEKHFAGEVKVVEQPKSAREYMSRQMDNWENLAVEGWFEGATPWMFYHKELKALMANIVGASAAEVCPMNTLTVNLHLLMVSFYRPTAKRFKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVAPRAGEATLRTEDILDTIATNGAETALVLFGGINYFTGQWYDMPAITKAGHHAGAVVGFDLAHAAGNVPLQLHNWDIDFACWCSYKYQNGGPGGISGIFVHEKHFAGEVKVDENTLTVTEPTTWGKVFKR"
  },
  {
    "reference": "MPPPLPEKAFPVSWDAFHRDARALAWRLADAGPFQAVVCVTRGGLVPAAIVARELNLRIIETVCVTSYGAPGTQGKLELLKPPAERIIGIAGGEGRGVVIVDDLVDTGGTARIVRGLLPKAHFAAVYAKPLGRPLVDTFITEVSQDTWIHFPWDTGLAFQPPLREGGA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that can perform hypoxanthine phosphoribosyltransferase activity, guanine phosphoribosyltransferase activity in plasma membrane to support XMP salvage, GMP salvage.2. For general function, the protein need meet that Purine salvage pathway enzyme that catalyzes the transfer of the ribosyl-5-phosphate group from 5-phospho-alpha-D-ribose 1- diphosphate (PRPP) to the N9 position of the 6-oxopurines guanine and xanthine to form the corresponding ribonucleotides GMP (guanosine 5'- monophosphate) and XMP (xanthosine 5'-monophosphate), with the release of PPi3. The protein should be optimized for activity in XMP from xanthine: step 1/1, XMP biosynthesis via salvage pathway pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The GMP, Mg(2+), 5-phospho-alpha-D-ribose 1-diphosphate, guanine, xanthine binding site should be stable and able to withstand changes in environmental conditions.5. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "MKAISWDQIHRDSRALAWRLDGQGPEEGAWRAVVAITRGGMAPAMIVARELDIRTVDTISIKSYDHQQQSEAKVLKAPDETLMGDGTGILIIDDLVDSGKTLEVVRAMYPNAHCATVYAKPKGEPQVNTFITGVSQDTWIFFPWDMALQYEETYQ",
    "response#2": "MWENLHREARRLSRRQLPASQWKGILAVSRGGLVPAAIMARELGIRFVDTICISSYEHDNQGDLNVLKRIDGDGEGFLIVDDLVDSGNTARLLREMYPKAKLVTVFAKPKGEHLVDDFEVAIPQDTWIEQPWDAQFAEDEAGLSRALAWRLDGHGPDNGAWKAVVAITRGGMAPAMIVARELDIRMVDTISVKSYHHQDQGEAQVLNEPDAGMMGDGTGVLVVDDLVDSGKTLELVRQKYPKAHFATVYAKPKGEDTYPLALEACTARALAWRLDRQGPGPDGSWRAVVAITRGGMAPAMIVARELDIRVVDTISVKSYDHQARSEAEVLKPPVAEMIGEGEGVLIIDDLVDTGKTLELVRRLYPKAHCATVYAKPKGRPMARDGLYLKGVHG",
    "response#3": "MNSWDQIHRDARALAWRLDRQGPGPDGSWRAVVAITRGGMAPAMIVARELDIRVVDTISVKSYDHQARSEAEVLKPPVAEMIGEGEGVLIIDDLVDTGKTLELVRRLYPKAHCATVYAKPKGRPMVDTYITEVSQDTWIFFPWDMALQYVTPYRGTDWENLHREARRLSRRQLPASQWKGILAVSRGGLVPAAIMARELGIRFVDTICISSYEHDNQGDLNVLKRIDGDGEGFLIVDDLVDSGNTARLLREMYPKAKLVTVFAKPKGEHLVDDFEVAIPQDTWIEQPWDREWREG"
  },
  {
    "reference": "MSVFAYPSETIREAEVPLLEAGVPLMARAARALANSAAAMLKADYGQVTGTRVSVLAGPGNNAGDALFAAATLGRRGAAVTIITLLDRTHGEGLTAARRAGAQVIAFTSSETEADAEAARADALRELSRAHLVLDGILGTGGRRGLPDHISSLIADWAPHRTGRLIAVDIPSDLDPNHTGAEKRLHADRTVTFGGLKTELIDPRVDEFTGTINVIDIGLGLDPRSAAAELLDRDDLVSEFPRPDATGHKYSRGVTGVLAGSEDYPGAGVLTTTSAVNTGAGMVRYLGSPLVAEYVLGLHPEVVSASGRIDAVVLGPGDSETEFVDNVAEALTGNRTPVVVDAGGLDMIARAESMAGTWLADRPLIITPHAAELARLLSRLLGEIITAGRIGANPMWWAQRAAELTGAIVLLKGHHTVIAAPDGHLVLPAPGPGSLATAGSGDVLAGILGTLAAITAAHSRYDSDDRMHPPREWARLAGLAVLLHNEAGRRAVTASGFADALAEVVGELIHGTDAVGSASSGPGSRSRYC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP; Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.2. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.3. The designed protein should be optimized for kinase activity, ATP binding, NADHX epimerase activity, NADPHX epimerase activity activity in order to improve phosphorylation.4. The protein should have a fast turnover rate for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "VMIGGDRGTAGAIRLAAEAAVRSGAGLVRVITHADNIGPLLTARPELMVDELSEHTLADALEWADVIVIGPGLGQGDWGKQALKKAENTAKPMLWDADALNLLAIRPDKRQNRIITPHPGEAARLLNVSIGEIEGDRVAAAQRLAKRYGGVVVLKGAGTIIASQQAELAIADVGNPGMASGGMGDVLAGIIGALTGQKLSLYDAACAGCVVHGAAADRVAGLKGPRGMLASDLFSDAGDISDLIKPRKPFTHKGTYGHALIVAGQRETMGAAILAARACLYSGAGLTTLSIPESGLTALNATLPEVMYKDRTALSQSGGGFENFSAIAVGPGLGTGAESLDILTSLLKLKLPLIIDADALNLLGEYEELFKQVPEGSVLTPHIKEFDHLFGVHDSWWDRLQTARAQAVQRKCVIVLKNQYTFIIDQYGGVKINPTGNPAMAQGGMGDVLTGIIASLSAQGYPADQAASAACYLHGLSGDKLAVSKIAVTASAVAGYIPEVLKELIEGSDVAAVLRVPGVRDHKYTRGVVGVVAGTEKYPGAGVLVTAGAVRAGAGMVRALGPTTVTDRILTRHPEVVTAGGRVQSWVLGPGIPSDGSDAGQDRRVYGALAAVTGVLDAEVTGGVVPAVIDAGAMAMLEHRLPEIAVLTPHARELAELMRGLGHEVDRADVEAEPVRWARAAQRATGATVLLKGSVTVIVGSGATFTQADAPAWLATAGAGDVLAGLLGTLLASRADDVLADPTLTSRVAAAAVVLHGRAAALASGGGPVSATDVADAVPRVIADVL",
    "response#2": "MATIDSDAQLQYKIPGLSLMETAGFKIWASLAAEIHKEQHLVFLCGGGNNGGDALVVARLAFDQGYVNQVCILIGNHISPSCAVQREIIQAYGLPSIMLGDEISPQVTRALLDAGVVFDGIAGTGLKGPLQGISKTLVALANKSKALRVAIDIPSGLGDMISPDEIHFLADRTCTLGLSKAAFYHPLSRNDCGQIEILNPSFPARLLEGSDVAAVLRVPGVRDHKYTRGVVGVVAGTEKYPGAGVLVTAGAVRAGAGMVRALGPTTVTDRILTRHPEVVTAGGRVQSWVLGPGIPSDGSDAGQDRRVYGALAAVTGVLDAEVTGGVVPAVIDAGAMAMLEHRLPEIAVLTPHARELAELMRGLGHEVDRADVEAEPVRWARAAQRATGATVLLKGSVTVIVGSGATFTQADAPAWLATAGAGDVLAGLLGTLLASRADDVLADPTLTSRVAAAAVVLHGRAAALASGGGPVSATDVADAVPRVIADVLEGSDVAAVLRVPGVRDHKYTRGVVGVVAGTEKYPGAGVLVTAGAVRAGAGMVRALGPTTVTDRILTRHPEVVTAGGRVQSWVLGPGIPSDGSDAGQDRRVYGALAAVTGVLDAEVTGGVVPAVIDAGAMAMLEHRLPEIAVLTPHARELAELMRGLGHEVDRADVEAEPVRWARAAQRATGATVLLKGSVTVIVGSGATFTQADAPAWLATAGAGDVLAGLLGTLLASRADDVLADPTLTSRVAAAAVVLHGRAAALASGGGPVSATDVADAVPRVIADVLRDAENAAFARGIPAEALMNQAGAGIARAVQQFFSRPGYCLVFSGKGNNGGDALVAAAHLKRAGWHIDVHLSFPESECSELLRKKVTEFRDTPAVAAAASAANADGTPAPIIILDGLLGLGSRPPLRDPIIAACREINRLRREQNAYVFAVDQPTGLDGDSGKADSDCVVADFTVTIAAAKRGLVADDATNFVGRLE",
    "response#3": "VMIGGDRGTAGAIRLAAEAAVRSGAGLVRVITHADNIGPLLTARPELMVDELSEHTLADALEWADVIVIGPGLGQGDWGKQALKKAENTAKPMLWDADALNLLAIRPDKRQNRIITPHPGEAARLLNVSIGEIEGDRVAAAQRLAKRYGGVVVLKGAGTIIASQQAELAIADVGNPGMASGGMGDVLAGIIGALTGQKLSLYDAACAGCVVHGAAADRVAGLKGPRGMLASDLFSFAGPGDVSIVVKPRRPEAHKGDFGRLLIVGGSETFSGAPALAALAALRTGVDLVYIAAPRETAYSISSMSPDLITLKLEGDHLSMRNMPIIRNYMEKVTAVAVGPGLGLHKETQEAVSEIIRIAEERKMPILLDADGLKAFAGFKRRLETPAVLTPHLGEYAILTSEKPPDNLEERGNHVKKTAENLNATILLKSHVDIISDGFKTKFNFTGNPGMTVGGTGDVLTGIVAAFLAQGVDPFEAAVAGAFINGAAGDFVKAEKGYHMVATDLLDWIPKVIS"
  },
  {
    "reference": "MKIVLLGPPGAGKGTQAKSISNRYSIPHISTGDIFRKNISENTPLGIEAKSYMDNGQLVPDEVTINMVKDRLQQDDCKNGYLLDGFPRTVHQAEALDNFLTEREESIDTALLIEVPKEFILERMTGRRVCPSCGASYHIKFNPPTNDGKCDLCGSDVIQRKDDTEETVKERLDVYENQTQPLIDFYKNKKQLSVVDGTQAINEVFESICKILGSDK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The desired protein should be able to perform AMP + ATP = 2 ADP2. For general function, the protein need meet that Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.3. Ideal protein should have a binding affinity for key enzymes in the AMP biosynthesis via salvage pathway, AMP from ADP: step 1/1 pathway to enhance the overall efficiency of the pathway.4. The designed protein must be optimized for zinc ion binding, adenylate kinase activity.5. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+), AMP binding. The designed protein sequence is ",
    "response#1": "MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETELGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSELDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQREDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILG",
    "response#2": "MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETELGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSELDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQREDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILG",
    "response#3": "MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETELGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSELDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQREDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILK"
  },
  {
    "reference": "MIELDAINPNNLKILEVINEKCFDPEIIIFPTSFYKDTISVGPLAQYAYFNQVCVGAVRCKKETHNKSHKIQILSLAVLPAYRNRSIGTKLLEYACETAAEGKAKEIYIKLSPKLDVSEWFIHRGFIIDESSKTEDSVLLSKKL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a specific function within the NatA complex, cytosol. The designed protein sequence is ",
    "response#1": "KMTKGLMYDPGDEELCKGRTEARQLIHRFNNSMDKLERKDITKQLFGSTI",
    "response#2": "REKMTKGLMYDPGDEELCKGRTEARQLIHRFNNSMDKLERKDITKQLFGSTGI",
    "response#3": "REKMTKGLMYDPGDEELCKGRTEARQLIHRFNNSMDKLERKDITKQLFGSTGL"
  },
  {
    "reference": "MIVSLIATVLFGYLLGSVSFSYIIAKKIKKIDIRSEGSGNAGATNTLRVLGIGPAICVLLLDVAKGVAPALLAIALTNGDYPLVPALAGLAAILGHNWPIYFGFRGGKGVATSIGVVATLLFLPALCAGIVAILSIVFTKYVSLGSLLFAVLTPIAALIMLPFFHYPLEYIYLAVLLAILSLWRHRTNMGRLIAGTENKLGKKHA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. We are looking for a protein with broad substrate specificity for an acyl phosphate + sn-glycerol 3-phosphate = a 1-acyl-sn-   glycero-3-phosphate + phosphate.2. The protein can be targeted to plasma membrane and perform acyl-phosphate glycerol-3-phosphate acyltransferase activity.3. For general function, the protein need meet that Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA)4. The protein should have Helical domains to ensure proper insertion into the membrane.5. A protein should be able to manipulate the activity of enzymes in the phospholipid metabolism, Lipid metabolism pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "GLRFEVKGRQKLEVDGPCVIISNHQSILDMMGLMEIVPARCVQVAKRELLFLGPVGLIMYLAGVYFINRQRSSTAVSVMTALGEHMIRENLKVWIYPEGTRNDNRDLLPFKKGAFYLAIQAQVPIIPVVRFEVKGRQKLEVDGPCVIISNHQSILDMMGLMEIVPARCVQVAKRELLFLGPVGLIMYLAGVYFINRQRSSTAVSVMTALGEHMIRENLKVWIYPEGTRNDNRDLLPFKKGAFYLAIQAQVPIIPVVYSSYSSFYSFREKRFTSGTCTIRVLPRIETTGLSPTDVPALTDTCHQAMLDVFSSLS",
    "response#2": "RFEVKGRQKLEVDGPCVIISNHQSILDMMGLMEIVPARCVQVAKRELLFLGPVGLIMYLAGVYFINRQRSSTAVSVMTALGEHMIRENLKVWIYPEGTRNDNRDLLPFKKGAFYLAIQAQVPIIPVVYSSFGDFFFLKKKRFTSGTIDVEVLPPVPTEGLPATDAEKVQMLCDHVMNIMSSGIK",
    "response#3": "GLRFEVKGRQKLEVDGPCVIISNHQSILDMMGLMEIVPARCVQVAKRELLFLGPVGLIMYLAGVYFINRQRSSTAVSVMTALGEHMIRENLKVWIYPEGTRNDNRDLLPFKKGAFYLAIQAQVPIIPVVRFEVKGRQKLEVDGPCVIISNHQSILDMMGLMEIVPARCVQVAKRELLFLGPVGLIMYLAGVYFINRQRSSTAVSVMTALGEHMIRENLKVWIYPEGTRNDNRDLLPFKKGAFYLAIQAQVPIIPVVYFSSFNNFYHRKEKRFLPGTIRLRILPKIETKGMTPDDVPHLTETVRKTMLETLSELS"
  },
  {
    "reference": "MKSSGPVERLLRALGRRDSSRATSRPRKAEPHSFREKVFRKKTPVCAVCKVTIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPAELRRSTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAAAFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVATELQPSQRRYVSYFSGLLSGSIRMNSSPLFLHYVFVPVLPAFEPNTGFQPFLKIYQSMQLVYTSGVYRIAGPGPQQLCISLEPALLLKGDVMVTCYHKGGQGTDRTLVFRVQFHTCTIHGSRLTFPKDQLDEAWADERFPFQASVEFVFSSSPEKVKGNTPRNDPSVSVDYNTTEPAVRWDSYENFNQHHEDSVDGALAHTRGPLDGSPYAQVQRVPRQTPPAPSPELPPPPMLSVSSDSGHSSTLTTEHTAESPGRPPPTAAERQELDRLLGGCGVASAGRGAGRETAILDDEEQPSVGGGLHLGMYSGHRPGLSRRCSCRQGFREPCGVPNGSYYRPEGTLERRRPPYGGYEGHPQGYAEASVEKRRLCRSLSEGPYPYAPELGKPANGDFGYRPAGYREVVILEDPGVPALCSCPACEEKLALPTAALYGLRLEREAAEGWSSEVGKPLLHPVRPGHPLPLLVPACGHHHAPMPDYGCLKPPKVGEEGHEGC",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein can be targeted to cytoplasm, plasma membrane and perform protein tyrosine phosphatase activity.2. The designed protein should contain one or more C2 tensin-type domains that are essential for its function.3. For general function, the protein need meet that Dephosphorylates nephrin NPHS1 in podocytes which affects mTORC1 complex activity ; Enhances RHOA activation in the presence of DLC1 4. The protein should have a low Km value for H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] +   phosphate. The designed protein sequence is ",
    "response#1": "TTAHNTSSTSVYISWKAPPPDTILGEFLGYRITYRPRDRDPNDTKEIYIRDNTVESHEIQNLQTYTQYKVTVQVFNPEGLGPETTIC",
    "response#2": "KIKEEFRLLEESVGPSETSEGDKKHNTSKNRYTNILPVNHTRVQLKKIQDKEGSDYINANYIDGAYPKQFICTQGPLPNTIADFWRMVWENRCRIIVMLSRESENCRIKCDRYWPEQIGGEQFSIYGNGNEVFGTYSVELVEVIQDPEREIITRNIRLTFEGETRDITQYQYEGWPDHNIPDHTQPFRQLLHSITNRQNQIIPSSDRNVPIIVHCSAGVGRTGTFCTAVIMMKKLDHYFKQLDATPIDQVVDPFTHLPITEYQSDNLDLKGLGYHFKSSIYNSNGINNNNNNNLNNNNNINNNSNGSNNTPQTEPNNEEDDDDAAESDLKYAIMDKYNSRIDFNLFSIVLKLREQRPGMVQQLEQYLFCYKTILDEIR",
    "response#3": "PVALLFHKMVFETVPMFSGGTCRDTSVKNKSEQIPHGFRKGNWHWDPQFVVYQLKVKIHTSNPVNTRKEEKYVFFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFVPGPEENFEKVENGTLPTETLPTETLPKEQAGIQTGGTVDNDKDYLILSLTKNDLDKANKDKANRYFSPNFKVKLYFTKC"
  },
  {
    "reference": "MIPAYTGTQIRAAEQPLLEAGEGPSLMRQAAFGLAQHTMDLLRARRGRVYGSSAAALVGTGNNGGDALLALALMARRGVACTAILTGEKAHAEGLAALRKAGGKLVRTVNSADTRQRSRSNRWREANQVIAGADVLIDAVLGTGARGAVELPKISPETLVVACDLPSGVDADTGQAHGGVLPANLTVTFGALKVGLAVGEGRLLAGRVEVVDIGLGPHLGKPELHVLEPGDAARTLPRPAQSVHKYSRGVLGLVAGSAQYPGAALLTATAAVNTGLGMLRTVSVPEIGPLLAQRVPEAIPAPDATAHVQAWAVGPGIGEDPLQAKNAAVAIGTGLPCVVDASALRTFQPEQGHGRLVLTPHAGELEDLLNRAGLRVDRTAIERDPLRWARWAAVGYSSVVLLKGPTTVCVSPDGYAVVISAGGPELATAGSGDVLTGILGALLAVQPVQPSPYELARLAATAVLVHGTAGRLAGPGPFGASGLAEAVPEALHAAAAGESVFNSTSRVINSGSKEIA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be optimized for ADP-dependent NAD(P)H-hydrate dehydratase activity.2. For general function, the protein need meet that Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP The designed protein sequence is ",
    "response#1": "PDSYKNAHGHVLLIGGNRGMAGAIRLAAEAALRAGAGLVSVATHPDNITSVLQGRFELMVHGVESTNELSPLMKKASVIVLGPGLGQDAWAKALFNCAMVTELPMVVDADGLNCLAHAPRQKSNWVLTPHLGEARRLLSHSKTPLNEHDRFAVAKKISHEYGAVTVLKGPGSLISEGERININRSGCAGMASAGMGDVLSGIIGGLIAQGMEAFAATNLAVYIHGLAAEQAAHDGEKGMLAGDLFEHIGPGDYRVFLPRYRERAHKGVHGRVMVIGGSSEFVGAPWLAALSSLYGGVDLVYLAAPAPVLSSIISPEIIPIKLHGERLVEAHVERLVSEIVSRRVHVLVLGPGAGRCEETINFFCKLYEKLTHLEHVSLLGIVVDADALYSSAQCLGHLQEVGRRYNVILTPHLGELKRLLGREIADTLEQRIEASKEAAKKTNSVVLLKGAIDVIASPNGKIKLNRTGTAQMTVGGTGDVLAGLVAAFLARGANPFYAAALAAFVNGLAGERVVKNVGRVTPMLLIEWLPRVQKELEQC",
    "response#2": "HPGLYSDHLPVADAAAHKYTHGHLAVFSGPLISSGAARLAAVAGLRAGAGLVTVASPPNAVLTQASHFTAIMQRALGGADALADWLGDRRLSAFVLGPGFGDADKARAFTRQLADTGRPVVLDADGITAHAACRDALGAAFGGAVRLALTPHEGEFARLFPDLAKDPALSKVDRARAAAAMLNAVVVYKGADTVIAAPDGRSAINADAPAWLATAGSGDVLAGILGGLLAQAMPVFEAACAAVAMHGLAARRAGIGMTAEDLAGQVRPPDHSAADAGRVFSGGPSSTGAARLSALAAARIGAGAVTILSPGNAMQVNAGHLTSIMLRKADTTADIDEFMSERQPSAYVLGPGFGFGDKARDFALWLLAQEPLYDMQRRIDLGGIVLDADGITAFRDDPAILFGAAGRVGAPSLIITPHEGEFGRLFPDIAGNKKSSKLAKARAAAKLANAVVVYKGADTVIAAPDGRAAINANGAPWLATAGSGDVLSGIAAGLLAQGMAPFQAACAAVWIHAEAGRRR",
    "response#3": "VFSGGPSSTGAARLSALAAARIGAGAVTILSPGNAMQVNAGHLTSIMLRKADTTADIDEFMSERQPSAYVLGPGFGFGDKARDFALWLLAQEPLYDMQRRIDLGGIVLDADGITAFRDDPAILFGAAGRVGAPSLIITPHEGEFGRLFPDIAGNKKSSKLAKARAAAKLANAVVVYKGADTVIAAPDGRAAINANGAPWLATAGSGDVLSGIAAGLLAQGMAPFQAACAAVWIHAEAGRRR"
  },
  {
    "reference": "MLRAHTVDQVRAAEAALMTTLPEGTLMQRAAHGLAYAVLDFLGGRGYGRRVLLLVGSGDNGGDALHAGVVLARRGVQVEAWLLGESTHDALAAFRAAGGRVGRPRGGRDFDVVVDGIVGIGGRPGLRSAAQEALAAVADVSVVAVDTPSGVGVDTGEIHGPHVHADLTVTFGTHKACHLLDPAAAASGAIHLVDIGLTLPPAQVEALQAADVASLVPRPEPDAHKYTRGVVGVRAGSAAYPGAALLSVAGASCGLAGMVRFVGDPTVADRVRDHHPEVVGAGRVQAWVVGSGADADAEAMLRAALEDDVPVVVDADALRHVDGPVGVPAVLTPHAGELAAMLDVERSEVEAAPLAHARSAAREYDAVVLLKGRRTTIARPDGLVRVTTTGTPWLATAGAGDVLGGLIGALLAAGLDPFDAASVGAWLHGAAATLASGGGPVVASDVATALPRVVATLPAP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have a low Km value for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.2. The designed protein must be able to perform ADP-dependent NAD(P)H-hydrate dehydratase activity, metal ion binding, NADPHX epimerase activity, NADHX epimerase activity, ATP binding under specific conditions.3. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.4. The protein should be able to bind K(+), AMP ligand in a variety of conditions5. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. The designed protein sequence is ",
    "response#1": "MRQAWRVADVRAAEKALMATLPAGTLMQRAAAGLARRCALLLSESGGVYGATVVLLVGSGDNGGDTLYAGATLASRGAGVRAILLRPDRVHLAGLAAFRRAGGVTVGDLPDRVDLVVDGIVGIGASGGLREPAAALVRRLPGLRGRGGARPPVVAVDVPSGVAVDTGDVPGDAVHADITVTFGCLKPAHVVGAAAVLAGHVELIDIGLDAADIASWWPRADAGSDKYSRGVVGIATGSATYPGAAVLSVTGALAGPTGMVRYAGSADRDVVHRHPSVVVAPRVADAGRVQAWVCGSGLGTDDGARTELRSVLATSLPVLLDADALTLLVGGQHATDLRRDAPLVITPHDGEFARLAGERPGADRVGAACRLAAWINAVVLLKGDRTIVATPSGEAWANPTGSPALATAGSGDVLAGLLGSLLAAGVPPERAAIMAAYVHGLAGRRAERDGPVTSPDVAAALRPVLDDATITGWMPQRDRHAHKYSVGLALVLGGSPGLTGAPVMASLAAARIGAGFVTCACASSIQPVLALKMTAITTLALPETADGGLDPDAALAALAPRLEKARALLVGPGLGRHPATQTFIRRLLTRTDLPTVIDADGLNALAGHLDDLAEQAAGRWILTPHAGEFRRLAGTDADLTDRIRTVQTCARRWNSVLLLKGLPGLVGTPDGRVYLNPTGNPAVATAGTGDVLAGLCVGLLAQGLPPARAAVAALYAGGAAADAYTTHRHPHTMTPLDLLEELPRVLHTLALPAQ",
    "response#2": "LMARAGEAVVAAIGDRWPAFARAPARALILCGPGNNGGDGFVIASRLQERGWTVTCVQADPSAEMPPDAAAAREDWQNRSDIKSFSDLEGTHWTVDLIVDALFGTGLTRALGGPFAELPAKIADARSAGTRVVAVDAPSGLCLDSGRVIGAQAVVADLTVQFAWAKADENWGKAALAPRRSTAHKNDFGHVLAVAGSEGKAGAATLVVLAALRAGAGLVTLAARPEVLRTALPGVPEAMGVPLPGNGPLGPGDLAPLRAALRGKTAFAMGPGIPRGPQTGQLLGELLAALDPGCATVVDADALNALAEERDAIAERLRKAPVRPVLTPHPGEFSRLTGEEVERVESDRIDAAVRASSRFGACVVLKGARTVVADPEGTSAVCAHGNPGMATAGAGDVLTGITAAVLARRSGGGSTGERARLAVFLHALAGDLAARRTGETGLVASDIAEVGLPRVFRRWQRELDRLAAEAGLPTRVLMESAGAAVAREVLALRPARVAVFCGPGNNGGDGFVCARFLRDALQSAGGSGEPVVVVATAREKLKGDALAAAQAWTGPVASVADAPRLGRGDAVVDAIFGSGLSRPPAGAEAAAIDAINEAGSRGAPVVSIDIPSGIDSDTGSVFPTHIASAHRTVTLHLPKRGLWLHPGAASAGEVRVAPIGIPRALEG",
    "response#3": "MSQQILNLDELSTLEHRYAGILGAGELMRRAGDAVARVIESRIAKPAHVVIVCGPGNNGGDGYAAALALQAKGYRVTCATVTGDAPKTDDAKAMYEAWVKTGGETVADPYSAGKADAVVDALFGTGLKKPLSGDWQDAAMWFNERQALHVSIDIPSGIDMMTGRWVGNARGCMADVTVSMLAPKAGCYMNDGADAAGTVLLDELDVSVPDSHKGTFGHLLVVAGSEGMTGAAALAGEAAMRVGSGLVSVAVPQSLRTVIEMKLTECLKLGLEESEPGVLGEGAVRQLTPHLHRYHATLLGPGLSTRGEVARVVVALIERAEHPMVLDADALNILAGTLESLEVAKAPLILTPHPGEMARLLRTSTARVQENRLSIAHDFATRHGVVLVLKGARTLIATPEGEVFVNPTGNAGMASGGTGDVLAGMIGGFLVQGHPPRTAALLGVYLHGLAGDLAAEALGERSFIASDLLDRLADAHKYKRGLLAVVGGAMPGAAILAATAAQGAGAGYVRLFAPDARNAPADIVVYAGALSDLLADERNRAVLVGMGLGRDAAARERLATALGSPAPMVVDADALMLLGPHLLAKRAAATICTPHEGELAALERAFELDGKGPRPTRALALARASGMIVVAKGPDTVIGAPDGRLACARRASSWLSTAGTGDVLAGTIASRLATGIEPFAAACEGVWLHGEAARLAGAAFTAGELL"
  },
  {
    "reference": "MNLMNATPTEYVWKYNPVSGIPAGAQQNYGATIDWVLPGGTGFAIATNDIRRQTLNPAVTRAITARFEAESDQQPYASPHETNVIAANVLDSGYPKSGLYPLELSGNQRVQLAGGLMVGRTEGRMQLAGGLTEGRVQLSGGFHGRPLVRGRSRRPPRWCGAELTGNGLPEQAEVTSDTYKYFLRTQGPSQVVEEPGVFSQRQFMTTFLPSVVPHPFDSTNPGDFPAQYSAIYKGRTAFEDTFWDW",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that [Hexon-linking protein-C]: Structural component of the virion that acts as a cement protein on the capsid interior and which glue the peripentonal hexons and group-of-nine hexons together.2. A protein that localizes to host cell nucleus, viral capsid and can perform hexon binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "LFPP",
    "response#2": "XNS",
    "response#3": "GRVXXFXRMX"
  },
  {
    "reference": "MTPHRLLPPLLLLLALLLAASPGGALARCPGCGQGVQAGCPGGCVEEEDGGSPAEGCAEAEGCLRREGQECGVYTPNCAPGLQCHPPKDDEAPLRALLLGRGRCLPARAPAVAEENPKESKPQAGTARPQDVNRRDQQRNPGTSTTPSQPNSAGVQDTEMGPCRRHLDSVLQQLQTEVYRGAQTLYVPNCDHRGFYRKRQCRSSQGQRRGPCWCVDRMGKSLPGSPDGNGSSSCPTGSSG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The ideal protein would be one that plays a critical role in positive regulation of MAPK cascade, negative regulation of cell population proliferation, regulation of insulin-like growth factor receptor signaling pathway, cell migration, negative regulation of canonical Wnt signaling pathway through insulin-like growth factor II binding, insulin-like growth factor I binding, signaling receptor binding, identical protein binding, fibronectin binding within Golgi apparatus, insulin-like growth factor binary complex, extracellular space, extracellular region.2. The protein must contain a signal peptide for proper functionality.3. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.4. The designed protein should contain one or more Thyroglobulin type-1, IGFBP N-terminal domains that are essential for its function.5. The engineered protein is expected to adopt a helix-turn-strand-strand-strand-turn-turn-strand-based conformation.6. The protein must exhibit the following characteristics: They alter the interaction of IGFs with their cell surface receptors; Activates the MAPK signaling pathway and induces cell migration.. The designed protein sequence is ",
    "response#1": "FRCPPCTAERLAACSPAARPPCPELVREPGCGCCPVCARLEDEACGVYTPRCAAGLRCYPDPGAELPPQALVQGQGTCAPCQQELDQVLERISTMRLPDERGPLEHLYSLHIPNCDKHGLYNLKQCKMSVNGQRGECWCVDPIHGKVIQGAPTIRGDPECRVRCEPCDARALAQCAPPPTAPACTELVREPGCGCCLTCALREGDACGVYTERCGTGLRCQPRPAEQYPLKALLNGRGFCAVRCEPCDARALAQCAPPPAASACSELVREPGCGCCLTCALREGQPCGVYTERCGAGLRCQPPPGEPRPLQALLDGRGLCTCRPAAPCPARCDVSRCPSPRCPGGYVPDLCNCCLVCAASEGEPCGGPLDSPCGESLECVRGLCRCLTPVVRCEPCDARALAQCAPPPTAPACTELVREPGCGCCLTCALREGDACGVYTERCGTGLRCQPRPAEQYPLKALLNGRGFCANAPCPARCDVSRCPSPRCPGGYVPDLCNCCLVCAASEGEPCGGPLDSPCGESLECVRGLCRTPVVRCEPCDARALAQCAPPPAASACSELVREPGCGCCLTCALREGQPCGVYTERCGAGLRCQPPPGEPRPLQALLDGRGLCTNCPARCDVSRCPSPRCPGGYVPDLCNCCLVCAASEGEPCGGPLDSPCGESLENECVRGLCRCRWSHAVCGTDGHTYANVCALQAASRRALQLSGTPVRQLQKGA",
    "response#2": "FKEVWQVNVKPRGLGQAKNLSGVYRLCLSSKAVHLVKLNSDVACVHLLLMNIRRCGHSENYFFIEVGRSSSTGAGELWMQVDDCVVAQHMHETFLDTMKALKAYKGAVSNGYDDISFKEVWQVNVKPRGLGQAKNLSGVYRLCLSSKAVHLVKLNSDVACVHLLLMNIRRCGHSENYFFIEVGRSSSTGAGELWMQVDDCVVAQHMHETFLDTMKALKAYDGVKGSASSTDPFGPSPGKPEIHKCRSPDKETFTCWWNPGTDGGLPTNYSLTYSKEGEKTTYECPDYKTSGPNSCFFSKQYTSIWKIYIITVNATNQMGSLSGAIRPGKPEIHKCRSPDKETFTCWWNPGTDGGLPTNYSLTYSKEGEKTTYECPDYKTSGPNSCFFSKQYTSIWKIYIITVNATNQMGSLSGAIRPGKPEIHKCRSPDKETFTCWWNPGTDGGLPTNYSLTYSKEGEKTTYECPDYKTSGPNSCFFSKQYTSIWKIYIITVNATNQMGSLSGAIQTTAPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGYDTKGKGDIHPHCQLQLDQVLERISGMHLPDDRGPLEHLYALPIPNCDKNGFFNLKQCKMSVNGQRGECWCVNPITGKVLPGSPTVRGDPEPCQQELDQVLERISTMRLPDDRGPLEHLYSLHIPNCDKHGLYNLKQCKMSLNGQRGECWCVNPNTGKPIQGAPTIRGDPE",
    "response#3": "FKEVWQVNVKPRGLGQAKNLSGVYRLCLSSKAVHLVKLNSDVACVHLLLMNIRRCGHSENYFFIEVGRSSSTGAGELWMQVDDCVVAQHMHETFLDTMKALKCKCQQEYFGERCNCADGYHGERCEISLGPVCGLFRCPPCTAERLAACSPAARPPCPELVREPGCGCCPVCARLEDEACGVYTPRCAAGLRCYPDPGAELPPQALVQGQGTCACKCQQEYFGERCELQSPPGKPEIHKCRSPDKETFTCWWNPGTDGGLPTNYSLTYSKEGEKTTYECPDYKTSGPNSCFFSKQYTSIWKIYIITVNATNQMGSSSSDPLYVCNCADGYHGERCEVSLGPVCGRSLCQLQLDQVLERISGMHLPDDRGPLEHLYALPIPNCDKNGFFNLKQCKMSVNGQRGECWCVNPITGKVLPGSPTVRGDPEPTPAAPCPARCDVSRCPSPRCPGGYVPDLCNCCLVCAASEGEPCGGPLDSPCGESLECVRGLCRCEQEAPPCQRELYKVLERLAAAQQKAGDEIYKFYLPNCNKNGFYHSKQCETSLDGEAGLCWCVYPWSGKKIPGSLETRGDPNPCQPPRNLTLEVKQLKDKKTYLWVKWSPPTITDVKTGWFTMEYEIRLKPEEAEEWEIHFTGHQTQFKVFDLYPGQKYLVQTRCKPDHGYWSRWSQESSLV"
  },
  {
    "reference": "MKKIPTKQTTIQGEVSLEGVGLHTGKNVRLTFKPAKANHGYAFQRVDLEGHPIIPADANLVTDTERGTTLEKNGVTIQTTEHVLAACVGLDIDNVLIELNASEPPIMDGSSKFFVEALEKAGKVELDAEREEFIVKDVISYADEDGSELILMPADNYQVTTMVDFGTKVLGTQNASIKSVKEFKNQIADARTFSFLHEIEMLLDNGLIKGGDLNNAIVYVDKELNPETVEKLKQAFGKEEIHIKSNGILDNLTLHYPNEAARHKLLDVLGDLALTGTRIRGKVIANKPGHTVNTQFAKKLSKIIKTEKRNKVPKIDLNTKPLFDVSEIMNRLPHRPPFLLVDKIYELSDKHVIGVKNVTMNEPFFIGHFPGKPVMPGVLQVEAMAQTGGILALSTVEDPENYLTFFMKMDNVKFKQKVEPGDTLIFKLELISPIRRGICHMQGYAYVNGKLASEAELMAQIVRQN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The Proton donor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "GEVYLVGAGPGDPDLLTFRALRLMQRADVVLYDRLVAPAIVDLVRRDAERIFVGKEKSNHVVPQPEINELLLRLAQQGKRVLRLKGGDPFIFGRGGEEIEDLVRHNIRFQVIPGITAAAGCASYAGIPLTHRDHAQSCLFVTGHLKDGSVDLNWNALCQPRQTVVVYMGLTGLEVICREMIRHGRERSTPAALIQQGTTLNQQVITATLESLPDAVAAAQVEAPTLLIVGSVVTLAP",
    "response#2": "GEVYLVGAGPGDPDLLTFRALRLMQRADVVLYDRLVAPAIVDLVRRDAERIFVGKEKSNHVVPQPEINELLLRLAQQGKRVLRLKGGDPFIFGRGGEEIEDLVRHNIRFQVIPGITAAAGCASYAGIPLTHRDHAQSCLFVTGHLKDGSVDLNWNALCQPRQTVVVYMGLTGLEVICREMIRHGRERSTPAALIQQGTTLNQQVITATLESLPDAVAAAQVEAPTLLIVGSVVTLAPA",
    "response#3": "GEVYLVGAGPGDPDLLTFRALRLMQRADVVLYDRLVAPAIVDLVRRDAERIFVGKEKSNHVVPQPEINELLLRLAQQGKRVLRLKGGDPFIFGRGGEEIEDLVRHNIRFQVIPGITAAAGCASYAGIPLTHRDHAQSCLFVTGHLKDGSVDLNWNALCQPRQTVVVYMGLTGLEVICREMIRHGRERSTPAALIQQGTTLNQQVITATLESLPDAVAAAQVEAPTLLIVGSVVTLHG"
  },
  {
    "reference": "MIMRLSKALVDMELADYSAALDPAYTTLEFENMQVLAMGSDVSPSEGVNLNAPNSLGVGALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFNRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSITALLQAEALSQQITSPVPGLSGDIRAKKIASIADVCESMKQQLLVLVEWAKFIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDHIVPRHCPELVEMNRVAMRILDELVLPFQELQIDDNEYACLKAIIFFDPDAKGLSDPSKIKRMRYQVQVSLEDYISDRQHDSRGRFGELLLLLPALQSITWQMIEQIQFVKLFGVAKVDNLLQEMLLGGTSNDGPHTHHPLHPHLMQENMGPNVIVANTMPSHLANGQMSTPETPQPSPPAGPGPDQYKLLPGAIATVAKQPGSIPQPAIAKQEVI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The ideal protein would be one that plays a critical role in transcription by RNA polymerase II, sex differentiation, negative regulation of cell population proliferation, cholesterol homeostasis through chromatin binding, fatty acid binding, DNA-binding transcription activator activity, RNA polymerase II-specific within cytoplasm. The designed protein sequence is ",
    "response#1": "SCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNATPLDKILHGSVGYLTPRSGGHLMNMKYYASPSDLLHDTTASPITLHEKNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPADNKWTPSFSAVTSANSVDLPACFFLKFPQPIPVSKAFVQKLQNCTGIPLFETPPTYLPLYELITQFELSKDPDPLPLNHNMRFYAALPGQQHCYFLNKDAPLPDGQSLQGTLVSKITFQHPGRVPLILNMIRHQVAYNTLIGSCVR",
    "response#2": "FGVQETAIISQEVHNFIMDQGWSEYQFCNQIWAGKCPKTIRMFYSNLYKKLSHRDAKSIYHHVRRAYP",
    "response#3": "SCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNATPLDKILHGSVGYLTPRSGGHLMNMKYYASPSDLLHDTTASPITLHEKNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPADNKWTPSFSAVTSANSVDLPACFFLKFPQPIPVSKAFVQKLQNCTGIPLFETPPTYLPLYELITQFELSKDPDPLPLNHNMRFYAALPGQQHCYFLNKDAPLPDGQSLQGTLVSKITFQHPGRVPLILNMIRHQVAYNTLIGSCVV"
  },
  {
    "reference": "MSIWFFRRTALHAALLSLPAFAISAQAADIPQVKITVNDKQCEPMALTVPAGKTQFIVHNVSQKGLEWEILKGVMVVEERENIAPGFTQKMTANLEPGEYDMTCGLLSNPKGKLTVAAGEQAPVKPDAMALVGPIAEYKVYVTQEVAQLVSQTKAFTDAVKAGDLALARKLYAPTRQHYERIEPIAELFSDLDGSIDAREDDFEQKSADPKFTGFHRLEKILFGDNTTKGADKFADLLYQDTLELQKRIAGLTFAPNKVVGGAAGLIEEVAASKISGEEDRYSRTDLWDFQANVDGAQKIVNLLRPLLEKADKPLLQKIDANFNTVDSVLAKYRTKEGYESYEKLTDADRNAMKGPITALAEDLAQLRGVLGLD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Involved in Fe(2+) uptake. The designed protein sequence is ",
    "response#1": "MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAPLGDPIHIETRRVSLVLRKKDLALLEVEA",
    "response#2": "PLAFAKEGEEVIVKKIDAGCGAMQRLVSMGINIGSKLKVIRNQNGPVIISTKGSNIAIGRGLAMKIMVK",
    "response#3": "PLAFAKEGEEVIVKKIDAGCGAMQRLVSMGINIGSKLKVIRNQNGPVIISTKGSNIAIGRGLAMKIMVE"
  },
  {
    "reference": "MDDQQPLQTYNQPPPPLPKHIQLQIQQRQLQLQQQEEQERLQQQQQQQQQQQDQQQHKLEEGSRRRSLSDVVGGVNISNKETPKRAFRIYWANDNHMYWTLLLDSTNTVGDVSEGLKDKVPSSGTQCYLYKRTKANFGRWKDRRLSNEDSVWDIVDRRRKNHKSDPIFVLKRKKSTFSISQKKSPQNESDTSPLSSSGEFLNPYTNPTGSNNIVPSNAGHSRNTSSNSNNSANTSSNNLLAHHQFNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNSNNSSNSNSNNSSNTTTTTTTTTTTTTTSTSTNSINTSNNNNNNNSGSNINPSSFISPMSSLVSLSLSSSPASSAPHSPTLSSQPSSPHTITPITTSPPPPQRNNSSKSLKSTMEMEPPAEYISLLSNNPPPTNGSGSRPPSGSFIPGSIVNGSRSSASDLIGFKPPVCQQQQQQQQQQQQQQQQQVQQQQQQPQQVQQLQQLQQLQQQYQQQQPQQQIQPLSPQQQSQQQQQSPVKQSESIPQSPNLQSITQSSSSNKPLSGSTGMNRSSSPSPFAKKNIGGGGGGGSLADRKNRLSVQPKDIMRLMRFYFGDSLDGTFFTIAVQNDTSAKDVCASVEQKLLLPSGSTFVSLVLPIQPNGSKIERVLGEDEIIIEVKDTWEDPSQTFFQVTHKQQAPNNKRLSRQIQAEPHVIPSEWRRSTDAISLRASSDDMWKRKSIPIFLQVTGPPNTHSHSHSPQRQLPNMSGSPHSHHHHQNNNNNNN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be optimized for stability and solubility when expressed in endocytic vesicle membrane, plasma membrane where it will be involved in chemotaxis, positive regulation of receptor signaling pathway via STAT, cellular response to nutrient.2. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.3. The designed protein must have at least one Protein kinase domain.4. For general function, the protein need meet that It is a downstream component of Rap1-regulated adhesion, and it is a regulator of myosin II phosphorylation; Serine/threonine kinase regulating cell substrate adhesion, phagocytosis, motility, chemotaxis, and actin cytoskeleton organization5. We need a protein that can perform ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-   threonyl-[protein] with high efficiency. The designed protein sequence is ",
    "response#1": "RFSPPLQNLTLYGDAFFRDRTISLTQQQPCFPSVTTPPSKPSSSGIGRALYVYPIKFLEPSTNTTASFSCRFSFSIIASPSCPFGDGFAFLITSNADSFVFSNGFLGLPNPDDSFIAVEFDTRFDPVHGDINDNHVGIDVSSIFSVSSVDAISKGFDLKSGKKMMAWIEYSDVLKLIRVWVGYSRVKPTSPVLSTQIDLSGKVKEYMHVGFSASNAGIGSALHIVERWKFRTFGSHSDAIQRCSS",
    "response#2": "LHINCGGEEVSIRNSLGKITYQTDNSRQTNAASNQQFDYWGVSNTGDFTDDNSDHDEYYTSTNLTLSGDYPDLYKTARRSALSLVYYAFCLENGNYNVKLHFMEIQFSDKEVYSRLGRRIFDVYVQGKLFLRDFNINKEANGNMKPVIKEINATVTNHMLEIRLYWAGKGTTLIPKRGNYGPLISACQGNSTGKDVNIFHPVTNVKLPDFYEYESSVDAEECHQSCLHNCSCLAFAYIHGIGCLIWNQNLMDAVQFSAGGEILSIRLAVPIEPQYLVLNTATIASDLTDFKNIWEDLTSRTITAASAARSTPSSSDNHYRVDFANLTKFQNIYALMQCTPDISSDECNNCLQRGVLEYQSCCGNNTGGYVMRPICFFRWQLFTDKICGGYTCCQAKIPADRPQVIGVDLESSGGNTTQGGNCKVAFLTNETYSPANVTEPEQFYTNGFTVIELGWYFDTSDSCLHINCGGEEVSIRNSLGKITYQTDNSRQTNAASNQQFDYWGVSNTGDFTDDNSDHDEYYTSTNLTLSGDYPDLYKTARRSALSLVYYAFCLENGNYNVKLHFMEIQFSDKEVYSRLGRRIFDVYVQGKLFLRDFNINKEANGNMKPVIKEINATVTNHMLEIRLYWAGKGTTLIPKRGNYGPLISALSGEDRVVWVANRETPTTDTSANLAISSNGSLLLFNGKHGVVWSIGENFASNGSRAELTDNGNLVVIDNASGRTLWESFEHFGDTMLPFSSLMYNLATGEKRVLTSW",
    "response#3": "SMEHKIGQGGFGSVYYAELRGEKTAIKKMGMQATQEFLAELKVLTHVHHLNLVRLIGYCVENCLFLVYEFIDNGNLSQHLQRTGYAPLSWATRVQIALDSARGLEYLHEHVVPVYVHRDIKSANILLDKDFRAKIADFGLAKLTEVGSMSQSLSTRVAGTFGYMPPEARYGEVSPKVDVYAFGVVLYELLSAKQAIVRSSESVSESKGLVFLFEEALSAPNPTEALDELIDPSLQGDYPVDSALKIASLAKSCTHEEPGMRPTMRSVVVLHINCGGEEVSIRNSLGKITYQTDNSRQTNAASNQQFDYWGVSNTGDFTDDNSDHDEYYTSTNLTLSGDYPDLYKTARRSALSLVYYAFCLENGNYNVKLHFMEIQFSDKEVYSRLGRRIFDVYVQGKLFLRDFNINKEANGNMKPVIKEINATVTNHMLEIRLYWAGKGTTLIPKRGNYGPLISAVK"
  },
  {
    "reference": "MLSAQADVRLVRGWERDRRRGRGDDEGAASDQKHGGADDESGAQEHRPPRVQEARARVPHRRPKPHQTCALAEVAEDHPEVDEEVEDAPPAGEQRRAHPLTEHRQAAIAAVHAANGAGDPENGERQDAPKRDLRRRRGPADHARSRTGSRRRRGRDPPRSVPPPLAVGRELARWLDGRGSRRRRGPRVLRRGGDVGSAGRSRNVCSALRFVHSPAFEAECYPGLRNILRIISPAREGEDLSQSLTAVILAAGHGTRMRSRTPKVLHAVCGRPMLDWVIDAVRDAGADKIKVVINPHHAEVAAHLAEKKLEATYQRDPRGTAHALQQVDGLEGDVLVVNGDSPLLTGETVRKVVDAHRRGKHLATLASVQDPSRDDGRIVRSKDGEFDKIVERKDAGDELRRSVHEFNVGVYCFDGARLREALAKISDDNQAKEYYLTDVFLHLKPVDIVELSDPDEAMGIKDRMRLAAASRIMQRRILDRLMERGVTIVDPDSTFIEAGVAIGQDTTIEPFSVIKGTTEIGIECRIGPHVHIEDATIGDRSDCGPFVKIRPGTAIAEDVHVGSFAELVRTKVGRGSKVPHVSYLGDTDLGEDANIGAGTITANFDGQRKNRTEIGDGAFVGVDTMLVAPVKLGKGARTGAGSVVTKDVPDGATAVGVPARVIRKKSPRPEGERGGEHQD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.2. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain..3. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.4. The ideal protein would be one that plays a critical role in regulation of cell shape, lipid A biosynthetic process, UDP-N-acetylglucosamine biosynthetic process, peptidoglycan biosynthetic process through UDP-N-acetylglucosamine diphosphorylase activity within cytoplasm.5. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "VILAAGKGTRMRSQLPKVLHGIAQKPMVKHVIDAAHELGAAGIHLVYGHGGELMQERLADESVYWALQAEQLGTGHAVAQAMPAVPDDALVLVLYGDVPLIRPQTLNTLLAGRQPGGDFGILTARFDDPTGYGRIVRNEAGDAVRIVEQKDASADELAICEVNTSVYVFDAAVLRESLAKVTTDNAQGEYYLTDVIGIAREAGGTVRALQTEDPMLVEGVNDRVQLATLGREFNRRICESHMRNGVTLADAHRFDLRGELVCGKDVYIDVNCVFKGKVVIGDGCSIGPNCILTDVSLGAGCEIYANSVLEGCNIANDCAIGPFARLRAGTELAANCKIGNFVETKKAVFDEGAKASHLSYLGDVSLGKKVNVGAGTITCNYDGVNKHKTVIEDGVFIGSDTQLVAPVIIGANATIGAGSTIRKNVPPGELTLTESKQRNIEGWVERKRPGSAAAQAAEKARAGQDDEG",
    "response#2": "VVIMAAGKGTRMKSSLPKVLHRLGGRALAAHVVDTAQRIGARHVVVVTGHGATDVEAALEGGASGRVPLFARQVPQLGTGHAMQQAAPLLPDDGTVVVLSGDVPLIGEATLRALVAQSGGRRLVGIVTTTLDDPTGYGRIVRDADGTARAIVEHKDADDAQRAIREVNTGILAAPTSVLRDWLARLRNDNAQGEYYLTDVVGLAVADGLTVAAHRPADPMEVEGANDRVQQARLERAHQARVAERLMRAELAAAEAQWQQLRREEAMEAGVTLRAPETVFFSYDTEIAADVTIEPNVVFGPGVTIASGATIRAFSHLEGAKVGEGCEVGPYARLRPGAVLEKGSKVGNFVEMKKAVLGEGAKANHLTYLGDAEIGAGANIGAGTITCNYDGYFKYKTVIGERAFIGSNSALIAPVTIGADAIVAAGSAVSRDVAPGDLRMVRAEPATVIARAELVRAEGIFQAKKRAEALENGVTMTAPETVFFGFDTYIGRDAVIEPNVVFGPGVTVESGATIRAFSHLEGCHVSRGAVVGPYARLRPGTELSENTKVGNFVEVKNATILEGAKVNHLSYIGDASIGARTNVGAGTITCNYDGFMKHRTEIGEGAFIGSNTMLVAPVKIGNEAMTATGSVINKDVPDGALALARARQRNIDGWVRPVRRG",
    "response#3": "MTAVILAAGKGTRMKSGLHKVLHQIAGRAMIDHLMASVDSLAPSHKVVVVGAGREQLETALGVRAKTCLQEPQLGTGHAVQQAQAQLGDFAGDVLVMYGDVPFVRTATMQAMLDRLHGDDNPAAVLTVLTTELDDATGYGRILRDADGTVTGIVEQKDATPAQRAVREINTGVFAFDGALLAEALGKVRTDNSQGEEHLTDVLGILRAAGHRVGASVAGDHREIAGINNRAQLAQARRLLNDRLLRAAMLGGVTLADAHRFDLRGELVCGKDVYIDVNCVFKGKVVIGDGCSIGPNCILTDVSLGAGCEIYANSVLEGCNIANDCAIGPFARLRAGTELAANCKIGNFVETKKAVFDEGAKASHLSYLGDVSLGKKVNVGAGTITCNYDGVNKHKTVIEDGVFIGSDTQLVAPVIIGANATIGAGSTIRKNVPPGELTLTESKQRNIEGWVERRRAGTKSAEAAERALAAAEPGDAAVDSAETDSAQPQE"
  },
  {
    "reference": "MWPFSRQQNVSRILPIVPTAPRRFPVWFPAVLVLVLIALDQWLKAWALAHLQLNAPAIPVIPGVLDWELTFNTGAAWSMFSGSAVPLALGRILVGLGILSYLLWKPQGRFLTVVLSMIAAGAIGNSIDGLQRGQVTDMIHSPLLSAVTEAINGTRFPIFNIADMCVVGGTILLLVASLLPERKREKAVPEA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that This protein specifically catalyzes the removal of signal peptides from prolipoproteins. The designed protein sequence is ",
    "response#1": "KIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQEDKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSDKIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKVIQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALDSLKKEKL",
    "response#2": "KKLDIPIYTAFKNTAASGGYYISAGSDKIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKVIQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALDSLKKEKKR",
    "response#3": "KIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQEDKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSDKIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKVIQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALDSLI"
  },
  {
    "reference": "MGGKWSKSSLVGWPAIRERMRRARAEPAAQAEPAAAGVGAVSRDLDKHGAITSSNTAATNPDKAWLEAQEEDEEVGFPVRPQVPLRPMTFKAALDLSHFLKEKGGLDGLIYSKQRQDILDLWVYNTQGYFPDWQNYTPGPGERFPLCFGWCFKLVPVDPQEVEEANEGENNCLLHPISQHGMEDEEREVLKWKFDSRLAFKHIARELHPEFYKDC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis2. The protein design must feature the PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif.3. The protein should be engineered to have a higher affinity for a specific molecule or receptor in virion component, host cell plasma membrane that is critical for suppression by virus of host autophagy. The designed protein sequence is ",
    "response#1": "GNKGTS",
    "response#2": "MKLGIVLSIV",
    "response#3": "LNLLSG"
  },
  {
    "reference": "MESSFSFGVILAVLTILIIAVNALVVVAMLLSIYKNDGVGLCFTLNLAVADTLIGVAISGLVTDQLSSSAQHTQKTLCSLRMAFVTSSAAASVLTVMLIAFDRYLAIKQPLRYFQIMNGLVAGGCIAGLWLISYLIGFLPLGVSIFQQTTYHGPCTFFAVFHPRFVLTLSCAGFFPAVLLFVFFYCDMLKIASVHSQHIRKMEHAGAMVGACRPPRPVNDFKAVRTVSVLIGSFTLSWSPFLITSIVQVACHKCCLYQVLEKYLWLLGVGNSLLNPLIYAYWQREVRQQLCHMALGLLADGSTQPQIETLKGKEERKKVGRKTLYTCDAQTLYTCDAQTLYTCDAQTLYTCDACDTQTLYTCDAQTLYTCDAQTLYTCDAQTLYTCDAQTLYTCDAQTLYTCDTQTLYTCDAQTLYTCDAQTLYTCDAQTLYTCDAQTLYTSSLVTGQTEQTPLKRANMSDPLRTCRG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The Extracellular, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.2. The protein should contain Helical; Name=3, Helical; Name=7, Helical; Name=2, Helical; Name=1, Helical; Name=5, Helical; Name=6, Helical; Name=4 transmembrane regions to facilitate transport across the membrane.3. The protein must exhibit the following characteristics: Receptor for the endogenous fatty-acid ethanolamide oleoylethanolamide (OEA) and lysophosphatidylcholine (LPC); The activity of this receptor is mediated by G proteins which activate adenylate cyclase; Seems to act through a G(s) mediated pathway.; Functions as a glucose-dependent insulinotropic receptor.4. A protein that contributes to insulin secretion, regulation of metabolic process, adenylate cyclase-activating G protein-coupled receptor signaling pathway by functioning in cytoplasm, plasma membrane, receptor complex with phosphatidylcholine binding, G protein-coupled receptor activity. The designed protein sequence is ",
    "response#1": "LNTVALWVFVHIPSNSTFIVYLKNTLVADLIMALMLPFKILSDSHLAPWQLRGFVCTLSSVVFYETMYVGIMMLGLIAFDRFLKIIMPFRKTFVKKTAFAKTVSISVWSLMFFISLPNMILNKEATPSSVKKCASLKSPLGLWWHQVVSHTCQFIFWAVFILMLLFYAVITKKVYNSYRKFRSKDSRHKRLEVKVFIVMAVFFVCFAPLHFVRIPYTYSQTTNKTDCRLENQLFIAKEATLFLATTNICMDPLIYTLTNKEMKRAFRILLCKKR",
    "response#2": "ANLLALRAFIRRVRQPHPAPIHVLLLSLTLADLLLLLLLPFKIVEAANNSRWLLGKATCALTVFGFYSSIYCSTWLLAAISIERYLGVAFPVQYKISRRPIYGLAAALVAWILSFGHCSVVIVVEYLEPKNKTSENQTDFECYNDFNDRQLHVVLSFRLELCLVLFCLPMAVTIFCYWRFVYLMLGQPQVGAKRQRRAVGLAVVTLLNFLLCFAPYNVSHVIGFYEGKIPSWRKYAVLFSALNAALDPLLFVFFNRLVSQAFRRSGAWGIVLEAVAGAGIVTTFVLTIILVASLPFVQDTKKRSLLGTQVFFLLGTLGLFCLVFACVVKPDFSTCASRRFLFGVLFAICFSCLAAHVFALNFLARKNHGPRGWVIFTVALLLTLVEVIINTEWLIITLVRGSGEGGPQGNSSAGWAVASPCAIANMDFVMALIYVMLLLLGAFLGAWPALCGRYKRWRKHGVFVLLTTATSVAIWVVWIVMYTYGNKQHNSPTWDDPTLAIALAANAWAFVLFYVIPKYSTVALVPITDDVPLTPFTYELYHSLCAIGPVLRLTSEVVSSLADLAANGVGAKADNLLLVINEYRGLSWSIGGDENITSVTTLPNILREFSSNLTGFSEGVGSEDSPKAFLNQAVAGAKSGDMVRQVRVLVDKMKEDVRIDFHNDWKVITMFIGGNDLCDFCTDSVIFSPRNVASRIRQALDILHSEVPRAIVNLIELLDIVPLRDLHRDKSLGCPTWFVGLICPCVLKPKEGSSELHKVEDFNRAYQHAMRELLDSGRYDTRDNFTVVLQPFMRDILLPKLENGRPDRSFFSPDCFHLSQKAHTLMARALWNNMLEPV",
    "response#3": "RSGAWGIVLEAVAGAGIVTTFVLTIILVASLPFVQDTKKRSLLGTQVFFLLGTLGLFCLVFACVVKPDFSTCASRRFLFGVLFAICFSCLAAHVFALNFLARKNHGPRGWVIFTVALLLTLVEVIINTEWLIITLVRGSGEGGPQGNSSAGWAVASPCAIANMDFVMALIYVMLLLLGAFLGAWPALCGRYKRWRKHGVFVLLTTATSVAIWVVWIVMYTYGNKQHNSPTWDDPTLAIALAANAWAFVLFYVIPLNTVALWVFVHIPSNSTFIVYLKNTLVADLIMALMLPFKILSDSHLAPWQLRGFVCTLSSVVFYETMYVGIMMLGLIAFDRFLKIIMPFRKTFVKKTAFAKTVSISVWSLMFFISLPNMILNKEATPSSVKKCASLKSPLGLWWHQVVSHTCQFIFWAVFILMLLFYAVITKKVYNSYRKFRSKDSRHKRLEVKVFIVMAVFFVCFAPLHFVRIPYTYSQTTNKTDCRLENQLFIAKEATLFLATTNICMDPLIYYCSSGRIQAARGYIVTDRDPLRPEEGRRLVEDVARLLQVPSSAFADVEVLGPAVTFKVSANVQNVTTEDVEKATVDNKDKLEETSGLKILQTGVGSS"
  },
  {
    "reference": "MNLEDYNEKLKLLKKSVQDIKIRNRNLLKQEAEEKEKGLKPRVYSMHDDEDDSDEKRSSDEQSDMRTNLFQYTVQDFIDWEKRQQRKKKSKLESKSGMRLQHLAKNTYDKELQNLPRPEFKMKAKETQFRINKDSGKVNIKDNKELVQRLSQNLKETAKQRYDSNRKIANDKEHTSLSGSINAKNLQFNKSLDDDHEAEK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Involved in pre-mRNA splicing.2. The designed protein's functionality will benefit from Polar residues, Basic and acidic residues compositional bias in its structure.3. The protein should be engineered to have a higher affinity for a specific molecule or receptor in U2-type prespliceosome, U2-type post-mRNA release spliceosomal complex, U2-type catalytic step 1 spliceosome, Prp19 complex, cytosol that is critical for mRNA splicing, via spliceosome.4. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "TPIQSQALPAIMSGRDVIGISKTGSGKTISYLLPLLRQVKAQRPLSKHETGPMGLILAPTRELALQIHEEVTKFTEADTSIRSVCCTGGSEMKKQITDLKRGTEIVVATPGRFIDILTLNDGKLLSTKRITFVVMDEADRLFDLGFEPQITQIMKTVRPDKQCVLFSATFPNKLTSVYQSISGNFSGTKQVEIVLNHGRSLELIRYDENGKMQSVLYTEVFGIVRSIIPFRLTSGTKDYIIVGSDSGRVVILEYNSQKNQFDKIHQETFGRSGCRRIVPGQYLAVDPKGRAFMIGAIEKQKLVYILNRDSSANLTISSPLEAHKSNTIVFSMCGVDVGFDNPIFATISVDYTEEDSSSGGGGGGSIEEMMDEDIGKKKKLLTYYELDLGLNNVVRKWSDQVDDSANIVMTVPGGTEGPGGVLVASEDYIVYRNQDHAEVRSRIPRRYGSDPNKGVLIISHSSHKQKGMFFFLVQSEHGDLYKITLDYQGDQVSEVNVNYFDTIVLANCLTVLKNGFLFAASEFGDHTLYFFKSIGDEEEEGSVYQSISGNFSGTKQVEIVLNHGRSLELIRYDENGKMQSVLYTEVFGIVRSIIPFRLTSGTKDYIIVGSDSGRVVILEYNSQKNQFDKIHQETFGRSGCRRIVPGQYLAVDPKGRAFMIGAIEKQKLVYILNRDSSANLTISSPLEAHKSNTIVFSMCGVDVGFDNPIFATISVDYTEEDSSSGGGGGGSIEEMMDEDIGKKKKLLTYYELDLGLNNVVRKWSDQVDDSANIVMTVPGGTEGPGGVLVASEDYIVYRNQDHAEVRSRIPRRYGSDPNKGVLIISHSSHKQKGMFFFLVQSEHGDLYKITLDYQGDQVSEVNVNYFDTIVLANCLTVLKNGFLFAASEFGDHTLYFFKSIGDEEEEG",
    "response#2": "ERSEFFDEVQSENDGQSSDVEEVDAKAIIFVSSQNICDFISKKLLNAGIVTCAIHAGKPYQERLMNLEKFKREKNSILLCTEVLSRGLNVPEVSLVIIYNAVKTFAQYVHTTGRTARGSRSGTAITLLLHDELSGAYILSKAMRDEEIKALDPLQAKELD",
    "response#3": "SVYQSISGNFSGTKQVEIVLNHGRSLELIRYDENGKMQSVLYTEVFGIVRSIIPFRLTSGTKDYIIVGSDSGRVVILEYNSQKNQFDKIHQETFGRSGCRRIVPGQYLAVDPKGRAFMIGAIEKQKLVYILNRDSSANLTISSPLEAHKSNTIVFSMCGVDVGFDNPIFATISVDYTEEDSSSGGGGGGSIEEMMDEDIGKKKKLLTYYELDLGLNNVVRKWSDQVDDSANIVMTVPGGTEGPGGVLVASEDYIVYRNQDHAEVRSRIPRRYGSDPNKGVLIISHSSHKQKGMFFFLVQSEHGDLYKITLDYQGDQVSEVNVNYFDTIVLANCLTVLKNGFLFAASEFGDHTLYFFKSIGDEEEEGXG"
  },
  {
    "reference": "MSTNPKPQRKTKRNTNRRPQDVKFPGGGQIVGGVYLLPRRGPRLGVRATRKTSERSQPRGRRQPIPKARRTEGRTWAQPGYPWPLYGNEGMGWAGWLLSPRGSRPSWGPTDPRRRSRNLGKVIDTLTCGFADLMGYIPLVGAPLGGAARALAHGVRVLEDGVNYATGNLPGCSFSIFLLALLSCLTIPASAIEVRNVSGVYHVTNDCSNTSIVYEAADMIMRTPGCVPCVRENNSSRCWVALTPTLAARNSSIPTTTIRRHVDLLVGTAAFCSAMYVGDLCGSVFLVSQLFTFSPRRHMTVQDCNCSIYPGHVSGHRMAWDMMMNWSPTTALVVSQLLRIPQAVVDMVAGAHWGVPAGLAYYSMVGNWAKVLIVMLLFAGVDGGTYTTGGAQAFNTRGIVGLFTAGPPQNIQLINTNGSWHINRTALNCNDSLNTGFFAALFYAHRFNASGCPERMASCRPIDKFAQGWGPITYAEPPILDQKPYCWHYAPRPCGIVPASEVCGPVYCFTPSPVVVGTTDRFGVPTYSWGENETDVLLLNNTRPPPGNWFGCTWMNATGFTKTCGGPPCNIGGAGNDTLICPTDCFRKHPEATYTKCGSGPWLTPRCMVDYPYRLWHYPCTVNFTIFKVRMYVGGVEHRLTAACNWTRGERCDLEDRDRSELSPLLLSTTEWQVLPCSFTTLPALSTGLIHLHRNIVDVQYLYGIGSAVVSYAIKWEYVLLLFLFLADARVCACLWMMLLIAQAEAALENLVVLNAASVAGAHGILSF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization.2. A protein that can perform RNA binding, cysteine-type endopeptidase activity, serine-type peptidase activity, ribonucleoside triphosphate phosphatase activity, structural molecule activity in viral envelope, lipid droplet, virion membrane, membrane, host cell endoplasmic reticulum membrane to support virion attachment to host cell, fusion of virus membrane with host endosome membrane, clathrin-dependent endocytosis of virus by host cell.3. The protein should be designed to catalyze the following reaction a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-   diphosphate + H(+) + phosphate.4. The designed protein have Helicase ATP-binding, Peptidase C18 domains with specific sequence motifs that are crucial for its activity.5. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "QVRNSSGLYHVTNDCPNSSIVYETADAILHSPGCVPCVREGNASKCWVAVAPTVATKDGKLPTTQLRRHIDLLAGGATLCSALYVGDLCGSVFLVGQLFTFSPRRHWTTQDCNCSIYPGHITGHRMAWDMMMNWSPTTALVVAQLLRVPQAILDMIAGAHWGVLAGVAYFSMVGNWAKVLVVLLLFAGVVLTGLMGRMVGSHYQLTNCCQEGDVLYSTKEFAYHLPGCVICTDDQCWDPVTVFLSVKPNGSTYDHHLRNHVLGLGVGAAVCDMLDINDQCGMLAVSLISLFDILPTHIQLNTTATCYLEVDYSPFGTIVGWVNWAVNEAGVVTWALSIVTRIPYAVFHLMELSSTPFLIAVVWFAIQGQWARLLLLAVLD",
    "response#2": "EVRNVSGVYHVTNDCSNASIVYEAADMIMHTPGCVPCVREDNSSRCWVALTPTLAARNSSIPTTTIRRHVDLLVGTAAFCSAMYVGDLCGSVFLVSQLFTFSPRRHETVQDCNCSIYPGHVSGHRMAWDMMMNWSPTTALVVSQLLRIPQAVLDVVAGAHWGVLAGLAYYSMVGNWAKVLIVMLLFAGVDATTHTVGGAQSYTTRAFTSIFSSGPAQKIQLVNTNGSWHINRTALNCNDSLQTGFLASLFYVHKFNASGCPQRMASCRPIDEFAQGWGPITYTEPDSSDQRPYCWHYAPRQCGIVPASTVCGPVYCFTPSPVVVGTTDRFGVPTYNWGENETDVLLLNNTRPPRGNWFGCTWMNTTGFTKTCGGPPCNIGGDGNNTLTCPTDCFRKHPEATYTKCGSGPWLTPRCLVDYPYRLWHYPCTVNFTIFKVRMYVGGVEHRLSAACNWTRGERCDLEDRDRSELSPLLLSTTEWQVLPCSFTTLPALSTGLIHLHQNIVDVQYLYGIGSAVASYVIKWEYVLLLFLLLADHVTGAVQGHAIRRLTSFFTPGPAQKIQLVNTNGSWHVNSTALNCNDSLQTGFIAALFYASKFNSSGCPERLASCRPISEFAQGWGPITYTEPDSLDQRPYCWHYAPRPCGIVPASEVCGPVYCFTPSPVVVGTTDRSGVPTYTWGENETDVLLLNNTRPPQGNWFGCTWMNSTGFTKTCGGPPCNIGGVGNNTLTCPTDCFRKHPEATYAKCGSGPWLTPRCMVDYPYRLWHYPCTVNFTVFKIRMYVGGVEHRLNAACNWTRGERCDVVDRDRSELSPLLLSTTEWQILPCSFTTLPALSTGLIHLHQNIVDVQYLYGVGSAVVSFAIKWEYVLLLFLLLAD",
    "response#3": "VTGGKAAQAAFTLTSLLSPGAKQNIQLINTNGSWHINRTALNCNASLDTGWIAGLFYFNKFNSSGCPERMASCRPLAAFDQGWGPISYANGSGPEHRPYCWHYPPKPCGIVPAKTVCGPVYCFTPSPVVVGTTNKLGAPTYNWGDNDTDVLVLNNTRPPLGNWFGCTWMNSSGFTKVCGAPPCVIGGVGNNTLHCPTDCFRKHPEATYSRCGSGPWITPRCMVHYPYRLWHYPCTINYTLFKVRMYVGGVEHRLEVACNWTRGERCDLDDRDRSELSPLLLSTTQWQVLPCSFTTLPALTTGLIHLHQNIVDVQYLYGVGSSIVSWAIKWEYVVLLFLLLADCWITCLVDARRHWCGSCVRDCWAETADDSLTFGNCGTGPRVTANLTAFPLHYGQKSTVALATKMVLTAKLQPLWKNLNTSIMCSVIRTSVHCFSCIGLPSPPAGLWERVPGDPISDCEGVQVSTGKRTPTCPTKQRWNATVHVCPGYAFYSPAYDDGELHVAGYWQWLLATAPFVVVRRDLHWLSILKVSMTGRADPPSPGQIASLRTALGTSMGCGVSGVLYATFHGTKGRALATPHGPRNPFWSSPSEDVVCYPLPHPLTSLEPCGCNSASRWVLTRAGSLVHGVAHGENRVQLDCPTPISKLKGASGTPVLCDCGHAVGVSGGTAGHTTRGFTSLFTLGPSQKIQLVNTNGSWHINRTALNCNDSLQTGFLAALFYAHRFNSSGCQERMASCRPLDSFAQGWGPITYAERDSPDQRPYCWHYAPRPCGIVPASQVCGPVYCFTPSPVVVGTTDRFGVPTYTWGENKTDVLLLNNTRPPQGNWFGCTWMNGTGFTKTCGGPPCNIGGSGNKTLTCPTDCFRKHPEATYTKCGSGPWLTPRCMVDYPYRLWHYPCTVNFTIFKVRMYVGGVEHRLNAACNWTRGERCNLEDRDRSELGPLLLSTTEWQILPCSFTTLPALSTGLIHLHQNTVDVQYLYGIGSAVVSIAIKWEYVVLLFLLLAD"
  },
  {
    "reference": "MKNLWIWSLPLIVLAFIGWQELANQMPVATSRMTYGRLLEYMQMGWVKRIDVYDRTALIEASSPETGWQWIRVDLPANSSDWLEQAKTLHIDVDVHAVSNWINVASNWIIPLIIIGVVIWLLSRSASSNTTGALNFGKSKARFQMVAKTGIMFDDVAGIEEAKEELAEVVAFLKNPSKFLAVGASIPKGVLLVGPPGTGKTLLAKAIAGEASVPFFSISGSEFVEMFVGVGASRVRDLFKKAKQNAPCLVFIDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNTGVIVIAATNRVDVLDAALLRPGRFDRQIMVSMPDVKSRIAILKVHANQKKLHPQVSLEAVARRTAGFAGADLANLLNEAAILAVRRGLKQITWKEIDDAIDRVIAGMEGTPIMDGKIKRLIAYHETGHALTATLLPNHPPVQKVTLIPRRQAKGLTWFMQDNERDLLSKSQLMSMIMVALGGRAAEEAVFGNAEVTTGASNDLQQVTNLARQMVTRFGMSSLGPLCLETGNEEIFLGRDMRLMPEVSEEVIAQIDAQVRGMIEACYEKVLELMQANRVVMDRIVEELMEKETLDGKEFRQLVSQAARLTAVN",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Zn(2+) is required for the proper folding and stability of the protein.2. The protein must be able to ATP binding. The designed protein sequence is ",
    "response#1": "FISPGWVDLHVHIWHGGTDISIRPSECGAERGVTTLVDAGSAGEANFHGFREYIIEPSRERIKAFLNLGSIGLVACNRVPELRDIKDIDLDRILECYAENSEHIVGLKVRASHVITGSWGVTPVKLGKKIAKILKVPMMVHVGEPPALYDEVLEILGPGDVVTHCFNGKSGSSIMEDEDLFNLAERCAGEGIRLDIGHGGASFSFKVAEAAIARGLLPFSISTDLHGHSMNFPVWDLATTMSKLLSVDMPFENVVEAVTRNPASVIRLDMENRLDVGQRADFTVFDLVDADLEATDSNGDVSRLKRLFEE",
    "response#2": "FGAAEAFLDDATAERDELRERVATLRADRQSAADRVAEAEALLDEGKCPECGQPVEGAPHVERVTDDRERVAELDAELADVEDELDAVAQRVDRGESLVT",
    "response#3": "FGAAEAFLDDATAERDELRERVATLRADRQSAADRVAEAEALLDEGKCPECGQPVEGAPHVERVTDDRERVAELDAELADVEDELDAVAQRVDRGESLVD"
  },
  {
    "reference": "MAHRRRCLLLLLAVLLPAMAARGDPDAVQDFCVPDAGRGRPVELAMLPAYPCRSPANLTAGDFAFSGVRAAGNFSPETGFAGVSVTPAQFPGLHTLGMSFARADLSAAGGVNPPHYHPRATETALVLAGRVYAGFVDSGGRLFAKVLEQGEVMVFPRAMVHFQLNVGDTPATVYGAFNSENPGIVRIPATVFGSGIREAVLERAFGLTPAELRRLEKRFGPPKKAEMED",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Mn(2+) binding site should be located in a region of the protein that is accessible to the ligand.2. For general function, the protein need meet that Probably has no oxalate oxidase activity even if the active site is conserved. The designed protein sequence is ",
    "response#1": "GLNTPLNTSNPKGIAANPANLLTFPGLNTLGISMYNVAIAPGGYNQPHSHPGVTEAGVVIEGSVLVGFLTTNYTLYSKVIGPGDMFVIPPGLIHYEGNVGKTQCRLLTVVADDLPSEVGVPHTLLATKPAIPNEVLISAFKADSKTY",
    "response#2": "SGLNTPLNTSNPKGIAANPANLLTFPGLNTLGISMYNVAIAPGGYNQPHSHPGVTEAGVVIEGSVLVGFLTTNYTLYSKVIGPGDMFVIPPGLIHYEGNVGKTQCRLLTVVADDLPSEVGVPHTLLATKPAIPNEVLISAFKADSKTIL",
    "response#3": "GLNTPLNTSNPKGIAANPANLLTFPGLNTLGISMYNVAIAPGGYNQPHSHPGVTEAGVVIEGSVLVGFLTTNYTLYSKVIGPGDMFVIPPGLIHYEGNVGKTQCRLLTVVADDLPSEVGVPHTLLATKPAIPNEVLISAFKADSKTL"
  },
  {
    "reference": "LVRPPYSYSALIAMAIQSAPRRRLTLSQIYQYVAGNFPFYKRTKAGWQNSIRHNLSLNDCFKKVPRDENDPGKGNYWMLDPNCEKMFDNGNFRRKRRRRGE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be optimized for RNA polymerase II cis-regulatory region sequence-specific DNA binding. The designed protein sequence is ",
    "response#1": "ILEDAPLWQRFKEVTNEMIVTKNGRRMFPVLKISVTGLDPNAMYSLLLDFVRTDSHRWKYVNGEWVPAGKPEVSSHSCVYIHPDSPNFGAHWMKAPISFSKVKLTNKLNGGGQIMLNSLHKYEPQVHIVRVGGAHRMVMNCSFPETQFIAVTAYQNEEITALKIKYNPFAKAFT",
    "response#2": "LEDAPLWQRFKEVTNEMIVTKNGRRMFPVLKISVTGLDPNAMYSLLLDFVRTDSHRWKYVNGEWVPAGKPEVSSHSCVYIHPDSPNFGAHWMKAPISFSKVKLTNKLNGGGQIMLNSLHKYEPQVHIVRVGGAHRMVMNCSFPETQFIAVTAYQNEEITALKIKYNPFAKAFLN",
    "response#3": "ILEDAPLWQRFKEVTNEMIVTKNGRRMFPVLKISVTGLDPNAMYSLLLDFVRTDSHRWKYVNGEWVPAGKPEVSSHSCVYIHPDSPNFGAHWMKAPISFSKVKLTNKLNGGGQIMLNSLHKYEPQVHIVRVGGAHRMVMNCSFPETQFIAVTAYQNEEITALKIKYNPFAKAFS"
  },
  {
    "reference": "PSSVSPQFTPAIRPFLQTISIGLVSFGEHYKRNETGLGVTASSLFTSGRFAIDPELRGAEFERIIQNLDVHFWKAFWNITEIEVLSSLANMTSATVRVSRLLSLPPEAFEMPLTADPKFTVTISPPLAHSAPGPVLVRLISYDLREGQDSEELSNLVKSEGPRSLELRPRPQQAPRAQSLVVHIHGGGFVAQTSKSHEPYLKSWAQELGVPILSIDYSLAPEAPFPRALEECFYAYCWAVKHCALLGSTGERICLAGDSAGGNLCFTVSLRAAAYGVRVPDGIMAAYPATMLQSTASPSRLLSLMDPLLPLSVLTKCVSAYAGGETEDPSDSDQKALGMMGLVRRDTALLFRDLRVGASSWLHSFLDLSGNKTHRNSVPTAEPMRRSVSEAALVQPEGPLGTDSLKSLTLHDLSLKCSSDTSDTPELSLSVETLGPPTPSDINFFVGPDDAPEAKAPDELSTKDRGCSASAAFPEGFHPRRSSHSTTRMPLCSSPIVKNPFMSPLLASDRMLQSLPPVHIVACALDPMLDDSVMFARRLRGLGKPVTLRVVEDLPHGFLSLAALCKET",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be optimized for activity in Lipid metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The presence of Hormone-sensitive lipase N-terminal, Alpha/beta hydrolase fold-3 domains is critical for the interaction of the designed protein with its intended targets.3. The protein can be targeted to caveola and perform acylglycerol lipase activity. The designed protein sequence is ",
    "response#1": "VFTALDKVCEENICVLSGPSESPYGTVTKRLVACMRKIRQHGRALEPVVASFTAVYHHYDFDAQTPANGYRTLVKVLQSCLVHIIHKGRYIASNCRGAFFRAEHNAAEMEAYCSALCQLRALLHLAQRLIHDNAHGHLYSPQDADLSDRFVQEYSSMHKACFYGRCLGFQFSPALRPFLQTIVISMVSYGETYGRQQSGIGTAALSLLTSGKYVIDPELRGAEFERITQNLDLHFWKSFWNVTESDILSGFTRIASNPVQVNLTLTVPPESLRLPLASDPTLSTSVSPPVAHWGPGPVHMRLISYDLRDGQVFATLERVCEDNISVLCGPSDLPYGSTANRLLTCIRQIQEHGRALEPVVGCFTAVYHHYDFDEQTPGNGYRTLVKVLLSCLFHIIQKARYVASNYNSAFFRAEHNAAEMEAYCSALCQLRALLHLAQRLINDNDFGQLYSLKDEDLSRRFVQEYSSMHKACFYGRCLGFQVF",
    "response#2": "VFTALYDVCEENAAFFSGATKGQAGDAAQRLVDAMTTIQEHARSLEPVISGFAAIYHHFDFDPHIPANGYRSLVKVVRCCLLHIIHKGRYITSNRRSIFFRVSHSATEMEVYCSALCQLRALLYLAQRLLHDNSQGNLFFQEESGISQSFLREYASMHKGCFYGRCVGFQSAVHLSVYLFVFLSGVAASSLLTSGKYAIDPDLRGQEYERITQNLDVHFWKTFWNITETESLVSMTATQVKVNRALSVPPMPFDLPLVADPTHTVTVSPPAAHIGPGPVQMRLISYELREGQQ",
    "response#3": "FTSLEAVCKENITALSSPPDLLYGDASRRLVTCMRKIQDHGQALKPVVSSFTAVYHHYDFDSDTPGNGYRTLVKVLRSCLLHIIQKGHYIASNYNGAFFRADHNASEMEAYCSALCQLRALLYLAQHLLHNNDHGQLYSLQDGELSKRFVREYTSMHKACFYGRCLGFQFLPTLRPFLQTVVISMVTFGERYGKQQSGFGMAAFSLLTSGKYVIDPELRGAEFERITQNLDMQFWKSFWNLTESELLSGLVSLASNLVQVNLTLTIPPEPLSLPLASDPKLFAPVSPPLAHWGPGPVHMRLISYELREGQL"
  },
  {
    "reference": "MIFEVKSPILGFESVAKMKLEKIDDLFMKLYNAEGEVPHFTLVNPFLLREYEFDVPASIKILLDLSDAKNLLIANIMVIQQPIENSTINFLAPLIFNFDNLTMAQVVLDSTQYPLYSLNEPIGKYYDKEEAQKGEQAAPVRDSQKK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm that is critical for regulation of translation.2. For general function, the protein need meet that Acts as an anti-CsrA protein, binds CsrA and prevents it from repressing translation of its target genes, one of which is flagellin; Binds to flagellin and participates in the assembly of the flagellum. The designed protein sequence is ",
    "response#1": "MRIER",
    "response#2": "SFR",
    "response#3": "MXPXGXN"
  },
  {
    "reference": "MSGAKFKFNTIEKAVADIKKGKMVILIDDEDRENEGDLTIAAEKVTPQAINFMAKYGRGLICLSLTPEKVDALKLPMMSDHNTSHFGTAFTVSIEAKKGVTTGISAADRAKTILTAIKPKTKPEDLSRPGHTFPLRAQPGGVLQRAGQTEGSVDLARLAGLSPAGVICEIMNDDGTMARVPQLAKFARKHSLKIITIKDLIEHRMRNECLVRRLATTKLPTEYGGEFTSVLFENIIDGAVHIALIKGDISASDKILVRVHSECLTGDVFSSRRCDCGDQLHKAMAMIKKEGKGVILYMRQEGRGIGLENKLKAYELQDKGLDTVEANVKLGFKPDLRDYGIGAQILVNLGIRKMRLITNNPKKIIGLGGYHLRIVERVPIEINPHEKNIKYLTTKKSKLGHLLNKV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have zinc ion binding, magnesium ion binding, manganese ion binding to facilitate riboflavin biosynthetic process.2. The protein should be able to bind D-ribulose 5-phosphate, GTP, Zn(2+) ligand in a variety of conditions3. The designed protein should interact with enzymes and substrates within the riboflavin biosynthesis, Cofactor biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "GDARVPTPWGEFRCTSYRSVRDGTEHVAFTLGDVAADEELLVRVHSECLTGDVFRSLRCDCGPQLEAAMARVAAAGRGAVVYLRGHEGRGIGLGHKLRAYELQDAGRDTVEANLDLGVPVDGREYGTGAQILADLGARRVRLLTNNPAKYGGLTGHGLEIVGRR",
    "response#2": "ERVVEVQMPTRYGDFRLVAYEERLTGDVHLALTKGTWSEDEPVLVRVHSQCVTGDIFGSKRCDCGDQLARAMQQVEREGRGVVLYMKQEGRGIGLLNKLRAYRLQEEGLDTVEANEALGFKMDHRDYGIGCQILRDLGIRKLRLMTNNPTKRIGLAGYGLEIVERVG",
    "response#3": "RVATAHLPTQWGHFIAHAYEADIEANPYIALVMGDVTSEEPVLVRIHSGCLTGDALRSLRCDCGDQLDLAMRRIAEEGRGVLLYIQQEGRGIGLVNKLKAYVLQDHGADTVEANELLGFPADIRDYSLGAQVLEDLGLRRIRLMTNNPAKFAALEGYDLEIVERVA"
  },
  {
    "reference": "MNNQWMSKIAVWGVILFVLFTVFKQFDKTSVPAGQIGYSEFLDEVRNRRIKSVVIQEGAGSIEIAATTTDDRKIRTTGTMLDRGLSGDLINSGVKFEVRPREEQGLLTSLLLSWGPMLLLVGVWIYFMRQMQGGGKGGAFSFGKSKARMLDEANNATTFADVAGCDEAKEEVKELVDFLKDPQKFQKLGGRIPRGVLLVGPPGTGKTLLAKAIAGEAKVPFFSISGSDFVEMFVGVGAARVRDMFEQAKKSAPCIIFVDEIDAVGRHRGAGLGGGNDEREQTLNQMLVEMDGFETNLGVIVMAATNRPDILDPALLRPGRFDRQVYVTLPDVRGREQILNVHMRKVPVGQDIRADILARGTPGFSGADLANLVNEAALFAARRNGRVVEMVDFEKAKDKIMMGPERKSMVMPEEERRNTAYHEAGHALVARLMPKTDPVHKVTVIPRGRALGVTMQLPEGDRYSMDKERMLSTISVLFGGRIAEEVFMHQMTTGASNDFERATAIARDMVTRYGMTDELGPMVYAENEGEVFLGRSVTKTTSMSEETMRTVDAVIRRIIDEQYSIARKHIEDNQDKMHAMAKALLEWETIDGEQIEDIMNGKPPRPPKDWTPSNNKGGGGPTPPVNPESAPAAA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must have a active site that is structurally stable and resistant to denaturation.2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.3. The ATP, Zn(2+) binding site should be stable and able to withstand changes in environmental conditions.4. The protein must exhibit the following characteristics: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins.5. A protein that can perform ATP binding, ATP hydrolysis activity, zinc ion binding, ATP-dependent peptidase activity, metalloendopeptidase activity in plasma membrane to support protein catabolic process, proteolysis.6. The presence of Zn(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MVMSEETKESTAYHEAGHAIVGRLVPEHDPVYKVSIIPRGRALGVTMYLPEQDRVSMSRQHLESMISSLYGGRLAEELIYGPDKVSTGASNDIERATDIARKMVTQWGFSEKLGPLLYAEDEGEVFLGRSVTQSKHMSDETARLIDSEIRELIDRNYTRARKIIEENMDIMHAMKDALMKYETIDAAQIDEFKSAVPNS",
    "response#2": "MVMSEETKESTAYHEAGHAIVGRLVPEHDPVYKVSIIPRGRALGVTMYLPEQDRVSMSRQHLESMISSLYGGRLAEELIYGPDKVSTGASNDIERATDIARKMVTQWGFSEKLGPLLYAEDEGEVFLGRSVTQSKHMSDETARLIDSEIRELIDRNYTRARKIIEENMDIMHAMKDALMKYETIDAAQIDAYRAMR",
    "response#3": "VLLVGPPGTGKTLLAKAVAGEANVPFFSMSGSDFVEMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAIGRARGRNVNFSANDERENTLNQLLTEMDGFASNQGVIILAATNRADILDRALMRAGRFDRQIHVLFLDPPKFEDYVKRILADAKVPWSKEVDLGRVAATTPGMVGADLANLCNEAALLAARREHEKVQMSDFLAKVAGVPPPPSLMKILEKVDLSTADVDASLIARGTPGFSGADLANLVNEAALVAARTAKKLVGMEQLKAALLVPPPEKKGEVVMTAGEKRSIALHEAGHATVSWFCEHANPLVKVSIVPRGQALGAAWYLPEERQITTKEQMLDEMCALLGGRAAEELFTGHISTGAMNDLERATKSAYGMVAYAGMSDKLPNICYYNNQEYQFTRPYSETTAKIMDDEVLKMINEEYDRAKALLQEHKEGHNRLAELLLSREVIYAEDVEARLGFPVRDW"
  },
  {
    "reference": "MDFTSDYAHRRMVKFLTIILIGFMTVFGLLANRYRAGRRERFRFSKANLAFASLWAIAFSLVYGRQIYKEYQEGQINLKDATTLYSYMNITVAVINYVSQMIISDHVAKVLSKVPFFDTLKEFRLDSRSLYISIVLALVKTVAFPLTIEVAFILQQRRQHPEMSLIWTLYRLFPLIISNFLNNCYFGAMVVVKEILYALNRRLEAQLQEVNLLQRKDQLKLYTKYYRMQRFCALADELDQLAYRYRLIYVHSGKYLTPMSLSMILSLICHLLGITVGFYSLYYAIADTLIMGKPYDGLGSLINLVFLSISLAEITLLTHLCNHLLVATRRSAVILQEMNLQHADSRYRQAVHGFTLLVTVTKYQIKPLGLYELDMRLISNVFSAVASFLLILVQADLSQRFKMQ",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that can perform sweet taste receptor activity in membrane, axon, dendrite, plasma membrane, neuronal cell body to support signal transduction.2. The protein should contain a Helical; Name=6, Helical; Name=2, Helical; Name=1, Helical; Name=5, Helical; Name=3, Helical; Name=4, Helical; Name=7 membrane-spanning region.3. The Cytoplasmic, Extracellular topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.4. The protein must exhibit the following characteristics: Probable gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.. The designed protein sequence is ",
    "response#1": "LEWHEAPTIAVALLAALGFLSTLAILVIFWRHFQTPIVRSAGGPMCFLMLTLLLVAYMVVPVYVGPPKVSTCLCRQALFPLCFTICISCIAVRSFQIVCAFKMASRFPRAYSYWVRYQGPYVSMAFITVLKMVIVVIGMLATGLSPTTRTDPDDPKITIVSCNPNYRNSLLFNTSLDLLLSVVGFSFAYMGKELPTNYNEAKFITLSMI",
    "response#2": "MQAMRFAVEEINNDSSLLPGVLLGYEIVDVCYISNNVQPVLYFLAHEDNLLPIQEDYSNYISRVVAVIGPDNSESVMTVANFLSLFLLPQITYSAISDELRDKVRFPALLRTTPSADHHIEAMVQLMLHFRWNWIIVLVSSDTYGRDNGQLLGERVARRDICIAFQETLPTLQPNQNMTSEERQRLVTIVDKLQQSTARVVVVFSPDLTLYHFFNEVLRQNFTGAVWIASESWAIDPVLHNLTELRHLGTFLGITIQSVPIPGFSEFREWGPQAGPPPLSRTSQSYTCNQECDNCLNATLSFNTILRLSGERVVYSVYSAVYAVAHALHSLLGCDKSTCTKRVVYPWQLLEEIWKVNFTLLDHQIFFDPQGDVALHLEIVQWQWR",
    "response#3": "LEWHEAPTIAVALLAALGFLSTLAILVIFWRHFQTPIVRSAGGPMCFLMLTLLLVAYMVVPVYVGPPKVSTCLCRQALFPLCFTICISCIAVRSFQIVCAFKMASRFPRAYSYWVRYQGPYVSMAFITVLKMVIVVIGMLATGLSPTTRTDPDDPKITIVSCNPNYRNSLLFNTSLDLLLSVVGFSFAYMGKELPTNYNEAKFITLSML"
  },
  {
    "reference": "MGDNVFLEPDPWASSSNWGSPVKPLNYKTAIGNSSIPLQYRNYWDVFQANNVLLFPEEESYSDIFHVPQDVMKEFFTLIESSSNQPLRQIQFFVLLALVACYQLGVPSTLEQIFKQRNVLPILQRFNPELFNRSSDNETPLFPNNSPPASTTALNLSSNIVPSINESKILEQEDDDVSNKSLPHAQQSIIRSFPDIQKQPKGFFSYPSSTVSSIAPSTLEAGNLHSQQPPKFSVDSSVDDNAITPRKPFSKIPNRLSPSTQPLLSNSRHSSFRLASSSTSFPASLEMNVDIDLEPSGYFFYRHNNYIISDSSNTREVLRRYSDFFWLHSYLMKKYPFRRVPLIPLKKFHSKCFNSKQFFRTPPPGLSDFVNDLSHHPIFSNDEVVRVFFTEPNVFKNWRRENQKRIDQEIEQFLVVPQSQVPDASETVKERLLKLNMSTTTAINNQLNIFRIFEKMIFTLQHFHEDFLRLQNSFNCLLDSGLYHQVFTSTFAQNESKIMSMASGHFYNIDSLLHQQNDAVKHTFLLGLSKEIKILISLRLLIERISEVFSTDLTKVRHTISNDENLLRETANSDESGRNRTFLNRSSKKRAENSLKSKKELYLKNLNQRYQIAHELEQELSYLQDYVFSLGNPYVEYCKQHVKLEEESLKIWHTLESDFSRLET",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.2. A protein that localizes to cytosol, Golgi apparatus, endosome and can perform phosphatidylinositol-3-phosphate binding with high specificity and efficiency.3. The presence of PX domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MHKASSSKKSFDDTIELKKNEQLLKLINSSEFTLHNCVELLCKHSENIGIHYYLCQKLATFPHSELQFYIPQLVQVLVTMETESMALEDLLLRLRAENPHFALLTFWQLQALLTDLSTDPASYGFQVARRVLC",
    "response#2": "DEKEWKTGKRKAEKDELVGVMIFSTMEPEAPDLDLIEIEQKCEAVGKFTKAMDDGVKELLTVGQEHWKRCTGPLPKEYQKIGKALQCLATVFSSSGYQGETDLNDAITEAGKTYEEIANLVAEQPKKDLHFLMECNHEYKGFLGCFPDIIGAHKGAIEKVKESDKLVATSKITPQDKQNMVKRVGIMSYALQAEMNHFHSNRIYDYNSVIRLYLEQQVQFYETIAEKLRQALSRR",
    "response#3": "MHKASSSKKSFDDTIELKKNEQLLKLINSSEFTLHNCVELLCKHSENIGIHYYLCQKLATFPHSELQFYIPQLVQVLVTMETESMALEDLLLRLRAENPHFALLTFWQLQALLTDLSTDPASYGFQVARRVLC"
  },
  {
    "reference": "MKIITSDEMRTLDKLAIEEHEISGEVLMERAGTGLANAIINMRPPRDKKILLIAGHGNNGGDAFVAARILVEEGYTCSLILACEESEIKGDAFTHFVKMRQDAGIIPIELTDVARWEKRKILCDVGLIVDGLIGTGLKGVPQGVVAAAIRYINKHLNCCKIVAIDVPSGLNADTGEAEGDCVFADRTVTMALPKIGLIQPRAIEYTGSITVVNIGFPQELIDKYKSDYELVTKEEVQRHFPRRARMSHKGNYGHLLVIAGSRGMAGAAGMALSAALRSGVGLLSAAVPESIAQTVTAWTKEAMVYPCPETENGAHTLELLDKLKNHINRYDAVLIGPGMGSEQETKSLIKVLLDTLTCPVLLDADGLNAYADSATELAKVKSGDLVLTPHPAELARILGVPTQEVQANRFSYARKSAQTTDSTVLLKGAGTLIAHEGNRLRINMSGNPGMATGGSGDVLAGLIAGFMAQGISGFDAAQVASFIHGCAGDFASFIDGEVSMTATDIIDAIPWVFLEMLPR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for (6S)-NADPHX binding.2. The protein must have high specificity towards ADP-dependent NAD(P)H-hydrate dehydratase activity, NADHX epimerase activity.3. The designed protein have YjeF N-terminal domains with specific sequence motifs that are crucial for its activity.4. The presence of Mg(2+) is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "LCVGGNAGMSGAIRLAGEAALRSGTGLVKVYAHTDSRIQICAGRPELMVICDGLQAALEWANCVVIGPGLGQDEWALKTFSMVIQYCHKHDVPIVIDADALNLLPKHATAFTSDQCVITPHSGEAGRLLESTIEEVESNRFVSARLLAQKYNATCILKGAGTIIDDGTQTWVCEHGNPGMATAGMGDVLSGIVGAMFAQRINKTDAAKYSVCIHGHAADIIAAEYGERGMLASDLFPF",
    "response#2": "MREVDGAAIEGVGIPGGHLMERAGVAVAAEILERHAPDEAVVFAGKGNNGGDGFVIARELSEAGVEVTVFLLAAPEEYRGDAKLNLDILANLGIDARRATDGSGALTEDAVVLTGMADVVVDAVFGTGFAGAAEGPAAAAIGLMNEAPGLVVSVDIASGVVASTGSVSGPAVAADLTVTLHAAKVGHFVTPGGSLSGEVVIAPIGIPALCDD",
    "response#3": "LVLAGSKEMTGAPVLSASAGLRVGCGLLTLAVPEPALPIVASKINEPVFWEWPAEEGYFARESYTLLKQRIASFDVVAIGPGLGTWEGGNNWLRKIINMVDVPLVLDADALNLLSENLSILREKKGQIILTPHPGEMGRLCGCTVAEIERDRVGYARSFAQDYEVYVVLKGTYTLIATPDGKVFINTMGSAALAKGGTGDVLTGMLAGMLAQKVAQDEAIEVGIQLAVYLHGIAGRICGEKSMYATLAGDVIDNC"
  },
  {
    "reference": "MQNHNTRYIFVTGGVLSSLGKGITSSSIATLLKHSGFEVSILKIDPYINLDPGTMSPLEHGEVFVTQDGAETDLDIGHYERFLNKSLEKKNNFTTGQIYLSVIEKERRGEYLGKTIQIVPHIVDEIKHRIKSVGEGSDFLVVELGGTVGDMEGMPYLEAMREMKHELGNERVLSIHVTLVPYIKTAEELKTKPTQHSVQELRRIGISPQIIVARSEKPLSKELKRKIALSCDVADDSVIVAEDAKSIYQVPLNFLQEDILTPIARNLSLPPLKPNMQEWDLLVKKIISPTKSVKIGFVGKYLSLKESYKSLTEALIHAGANLDAKVEIEWIDSENLTSNSSILQDLDGILIPGGFGERGVEGKILAIQYAREHNIPFLGICLGMQLSIIEFARNVLGIKEATSSEFDSKTSYPFIYMIEDFIDQEGQKQIRTHTSPLGGTMRLGEYECNLKENSLLQKAYNNQKTIKERHRHRYEANPKYRSMLEEKGLIISGESNGLIEAIELANHPWFVAVQFHPEFTSRLQNPNPVILEFVKQSLKKC",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a high specificity for L-glutamine, ATP binding, with minimal off-target effects.2. The designed protein must possess metal ion binding to support glutamine metabolic process under certain conditions.3. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen4. The protein with the reaction H2O + L-glutamine = L-glutamate + NH4(+), the Nucleophile; for glutamine hydrolysis, Nucleophile must be optimized to ensure efficient catalysis.5. The designed protein should interact with enzymes and substrates within the CTP biosynthesis via de novo pathway, CTP from UDP: step 2/2, Pyrimidine metabolism pathway to enhance or regulate its activity.6. The designed protein must have at least one CTP synthase N-terminal, Glutamine amidotransferase domain. The designed protein sequence is ",
    "response#1": "MQDAYKSIIESFIHAGAENDARVKLHWISAEDLETEKPETLLKGMYGILIPGGFGERGVEGKINAITFARENHIPFFGICLGMQCAVIEYARNVCGITKAQSSEFHKTRFNVIDIMPDQKNIKNIGGTMRLGAYPCIIEKNTKAYEAYKSEYITERHRHRYEVNNKFRAALQSEGLIFSGLSPNRELVEMIELPGHPWFVGCQFHPELKSRATKAHPLFRDFVTRITTEDSYKSLNEALTHGGVANNLKVNIRWVESEHLVTDEQWEEKLQDVDAILVPGGFGRRGIAGMIKAIGYARRVKIPFFGICLGMQCACIEFARNVCGLTDADSTEFNPSTPHPIFYKLRDLIGVEEMGGTMRLGAYDCVLQPGSLAHRVYGTDLISERHRHRYEFNPEYEPVFSQHGMIFSGKSPDGRFLEIVELADHPWFLGCQFHPELKSKPLDPHPLFVSFVLPDAYLSVTEALRHAGWAHERNLRIRWVDSEALTPENTADRLGGVAGVVVPGGFGHRGIEGKVLAARHARENDVPYLGLCLGLQCGVIEFARDVLGKADANSTEFDMFTDHPVIDFMPDQRTMEDKGGTMRLGLYPARLAPGTRAAEAYGAEVIYERHRHRFEVNNEYRDALEAAGMVLSGLSPDGRLVEIVELRDHPWFVASQFHPEFKSRPDRPHPLFRAFVEQTLARLG",
    "response#2": "MQDAYKSIIESFIHAGAENDARVKLHWISAEDLETEKPETLLKGMYGILIPGGFGERGVEGKINAITFARENHIPFFGICLGMQCAVIEYARNVCGITKAQSSEFHKTRFNVIDIMPDQKNIKNIGGTMRLGAYPCIIEKNTKAYEAYKSEYITERHRHRYEVNNKFRAALQSEGLIFSGLSPNRELVEMIELPGHPWFVGCQFHPELKSRATKAHPLFRDFVESIAANVGDAYKSIVEAFVHAGAENDVKVSIRWVNAEDCEEGDLNEFFAGVSGILVPGGFGVRGIEGKINAVNYARVNKIPFLGICLGMQCAVIEFARNVCNLPKANSSEFKKGAYSVIDLMPGQKGIKNMGGSMRLGSYNCRLKKNTKAAEAYQEVNVNERHRHRYELNNQFRELLVEKGLVISGTSPDEQLVEIIELKDHPWFVGCQFHPELKSRATRSHPLFREFVESIAANVG",
    "response#3": "MQDAYKSIIESFIHAGAENDARVKLHWISAEDLETEKPETLLKGMYGILIPGGFGERGVEGKINAITFARENHIPFFGICLGMQCAVIEYARNVCGITKAQSSEFHKTRFNVIDIMPDQKNIKNIGGTMRLGAYPCIIEKNTKAYEAYKSEYITERHRHRYEVNNKFRAALQSEGLIFSGLSPNRELVEMIELPGHPWFVGCQFHPELKSRATKAHPLFRDFVIDGMKDF"
  },
  {
    "reference": "MSHRKFSAPRHGSMGFTPKKRSKRHRGKVKAFPKDDPTKPIHLTAFIAFKAGMTHIVREVDKPGSKVNKKEVVEAVTIFEAPPMVIVGIVGYIDTPRGPRQFKTVWAEHLSEDCRGRFYKNWHTSKKKAFQKHAKKWQDEDGRKSIEADLNKMKKYCSKIRVIAHTQMKVMKHREKKAHIMEIQINGGTVAEKVDWAREHLEKQVPVDSVFAQDEMIDCIGVTKGKGFKGVTSRWHTKKLPRKTHKGLRKVACIGAWHPSRVQFTVARAGQKGYHHRTEVNKKIYRLGKSCLTEEGKKNGGTDYDITEKSVNPMGGFPHYGLVNQDFVMIRGCCVGSKKRPITLRKSLIVQTKRFAHEKINLKWIDTSSKFGHGRFQTHAEKRAFMGKLKKDLIAESEAVKT",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have structural constituent of ribosome within ribonucleoprotein complex, ribosome to regulate translation. The designed protein sequence is ",
    "response#1": "IGNITKIKNVPEGMAVNSVESVYGDGGTFAMVNGSYSLVVNHRKETNETVIKIPSGKKLTVSSECRCIVGVVAGGGIHDKPLLKASVAHYKAKARGHVFPRVRGVAMNPVEHIHGGGNHQHVGKPTTVG",
    "response#2": "IDIGNITKIKNVPEGMAVNSVESVYGDGGTFAMVNGSYSLVVNHRKETNETVIKIPSGKKLTVSSECRCIVGVVAGGGIHDKPLLKASVAHYKAKARGHVFPRVRGVAMNPVEHIHGGGNHQHVGKPTTVSKKDIL",
    "response#3": "IGNITKIKNVPEGMAVNSVESVYGDGGTFAMVNGSYSLVVNHRKETNETVIKIPSGKKLTVSSECRCIVGVVAGGGIHDKPLLKASVAHYKAKARGHVFPRVRGVAMNPVEHIHGGGNHQHVGKPTTVG"
  },
  {
    "reference": "MTVEYKRRPTFWEIFKARCSTADLGPISLNWFEELFSEAPPYNTEHPEESEYKPQGHEPQLFKTPQRNPSYHQFASTPIMFKEQSQTLPLDQSPFKELGNVVANSKRKHHSKKKARKDPVVDVASLPLKACPSESPCTPRCTQVAPQRRKPVVSGSLFYTPKLEETPKHISESLGVEVDPDMSWTSSLATPPTLSSTVLIARDEEAHRNAFPADSPASLKSYFSNHNESLKKNDRFIPSVSDSENKSQQEAFSQGLEKMLGDSSSKINRFRDCLRKPIPNVLEDGETAVDTSGEDSFSLCFPKRRTRNLQKTRMGKMKKKIFSETRTDGLSEEARGQADDKNSFALEIEPRDSEPLDPSVTNQKPLYSQSGDISSEAGQCSDSIWSQPDPSGLNGTQTRKIPLLHISFHKQSILEDFIDMKKEGTGSITFPHISSLPEPEKMFSEETLVDKEHEGQHLESLEDSISGKQMVSGTSQTACLSPSIRKSIVKMREPLEETLDTVFSDSMTSSAFTEELDASAGGLEIHTACSQREDSLCPSSVDTGSWPTTLTDTSATVKNAGLITTLKNKRRKFIYSVSDDASHQGKKLQTQRQSELTNLSAPFEASAFEVPFPFTNVDSGIPDSSIKRSNLPNDPEEPSLSLTNSFVTAASKEISYIHALISQDLNDKEAILSEEKPQPYTALEADFLSCLPERSCENDQKSPKVSDRKEKVLVSACRPSGRLAAAVQLSSISFDSQENPLGSHNVTSTLKLTPSPKTPLSKPVVVSR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA)2. The designed protein should have a specific function within the lateral element, DNA repair complex. The designed protein sequence is ",
    "response#1": "PPCFSCAFDNREGKFSGESYLASGALKRLILNLDPLPTNFEEDTVELFGFQWVTETALVYSCRELFHLFRQQIFNLESLVQVSCDFGKIATLHAKADSIRQQCVVFLHYIKVFIFRCLKVQEAESHSRPAHPYEALEAQLPSMLVDELRGLLLYIGHLAALPSVTVGAFVNQNQMKLFPPSWHLLHLYLDTHWLVLEILHILGEKLKQVVYGRQFIGQAGDNLTNVSLFEEHCEHLFCDLICLSLNRFDKVMPSEALLISHCPCSCVKELWVLLIHLLDHRRKWSVADSFWNWLNKLLRTLFEKSSDQRRSSVSLTQAKDPLGFSWWISVHVASLYQIDRHGVSDKMKQMESNWSFIEELLKRSVTVQDSILEEQLRMHLHCCLTLCDFWEPNISVVTILWEYYSKNLNSSFSISWLPLKGLTNIIKSPLSMLTLVRNCCSDKQDPDLYKSSSSYIIFLCILAKVVKKAMRTSGPHPWKQVKGRIYSKFHQKRMEELTEVGLQNFFSLFLLLAAVAEIEDVASHVLDLLRFLRPASMSSHGALVWKGQMAFLLMYAQKNLDIGVWAEKLSCEFQEKAKEFLVSKNDEMVQRHALWTLLCIYIDGVQEVFETSSCLYPSHEHLLNDGFSMLLPACRESELRTVLNFLQAVLARIRSVHQQLCQELQRENVDLTVQSSLSAKERPLAAVAGALWRHFFSFLKSQRMS",
    "response#2": "EVIKFSSTQSELDIVLGGGIAKGGLYLVGGSPGVGKSTLLLKVASGLAKNQQKVLYVSGEESLSQIKMRAIRLDCIEKELYLLNEINWPVIKANIESENYFACVIDSIQTLYSPEISSAPGSISQVREITFELMRLAKTRDIAIFIIGHR",
    "response#3": "MHQTTLEFNRKALEIAERDSCCYLCSRKFENESFKSKLLQELKTKTDANFEKL"
  },
  {
    "reference": "MILQIDMGNTRLKWRVKNKLASLVEGHCLWSDAEDALMASLMPYGGLISRILVASVRSQEDNQAFMQLLGRLTPLVPEFAYSQSHNDGLVNGYADPERLGVDRWLALLAGYDRHKAYPFMLMSAGTALTLDLVDGQGHHLGGYIAPGLDVFVRAVSHSAAQINVVKSNNLFDASPGRSTVDAVHHAFAAMLSGLVQKSYELLSRNNGFAPVLLITGGDADVVKGLFHQGITCPDLVFSGLDIYFDLRAGNNG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a high specificity for substrate binding, with minimal off-target effects.2. A protein should be able to manipulate the activity of enzymes in the coenzyme A biosynthesis, Cofactor biosynthesis, CoA from (R)- pantothenate: step 1/5 pathway, either by activating or inhibiting their function.3. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for (R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP +   H(+).4. The protein design must be able to perform pantothenate kinase activity in order to contribute to phosphorylation, coenzyme A biosynthetic process.5. For general function, the protein need meet that Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis.6. K(+), NH4(+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "MRGPAXDVAXXHAAPXPXRXGXSXAXGXGXGXXXGKXXRXXYXXGXXM",
    "response#2": "MNNEALKGKKLGIMGLGVSGRAAVKLALSQGAEVFAWDDNAEKRAAAESLGGELVDFRDADWQALDVLVLSPGVPTLHPDLQPLRERASQGIELVGDLFRLAQAAEAQGRPFVAILGNNGKTTVTGMLGHMAQAAGRPVAVGGNIGTPALDLLEGGVDAYVLEVSSFQLDLTPALRFDVAVLLNLTEDHLDRHGSMDAHRAAKRRIFAGQECAIFNRDDPGTAYMAGDSQARRFGFSLRPGEVDLREDALYLDGVRHHLIGSHNMANILAAMAMVRALGFEADAIRDALARFPGVPHRLQPVAQFHGVDFINDSKATNADAALRALEGYDQPVILIAGGRDKGGDYAPLLPLVKGKVKAVLMFGEAREVLARELGGVPVTLAENLPDAVRLAREEAAAGGVVLLSPACASFDQYRNFEERGDHFVRAVAAL",
    "response#3": "MVAVLRPADPTPPTGATAVPFGAVDELIARIAAGEFVIVADDENRENEGDLLMAAEKATPETINFMITHARGLVCAPLTGEDCDRLGLLPMVTDNTDAQGTAFTVTIDAAHGVTTGISAADRARTIRLLADPSAAPADLVRPGHVFPLRARPGGVLARAGHTEAAVDLARLAGLRPAGVIAEIVGEDGSMARLPQLVEFARTHRLKIITIADLIQHRRRTERLVTRSSPVQPAVTPVRAYLPTTKQRKLGHLTLP"
  },
  {
    "reference": "MEHLQNTNIILGVSGSIAAYKAPLLVRALRQLGAQVRVAMTPSARQFVSPLVLGNLSGEPVALDMFDEKIQQGGSWHIHWAQWAHAMLVAPCSAHLLASLAIGLCDSIVGALALALPRSVPLLVAPAMDTTMWEHPATQRNVEQLRRDGAIVIPPDEGELASGLWGVGRLPEPETLLLYLDRALGGHQQTATNSKDTRIEAVLSRSTMPLAEAVAADRLTAEIELAQLKGSTHGRLDGKTVLITAGPTREALDAVRFISNRSSGRMGYALAEASRDRGARVVLISGPTAVESPEGIECIRVESAHQMLDAVRQYRGQWDVAIAAAAVADYAPAEPAHGKLKRQQLGERLTIELVRTPDILAELSNAKRTDAVLVGFALESSQHLDDGIEKLRSRGCDIAVLNAFDAPNSGFEGELNTITVAIIEGESVVRHAYQPSPKRVCAEHILHHVISLLQQRQQKH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein have Flavoprotein domains with specific sequence motifs that are crucial for its activity.2. The protein must exhibit phosphopantothenate--cysteine ligase activity. The designed protein sequence is ",
    "response#1": "VGRRIVVSAGGTREAIDPVRYVGNRSSGKMGYAVAEAARDRGAEAVIVSTVSLPVPAGVRVVAVESALQMMEAVHRECADADALIMAGAVADYRPADAADQKIKRDKTGDMSIDLLENPDIIATTPIETKRGRLTKISFAAETQDLIANAKAKLTKKGARFVVANDVSATDAGFGVDNNRVTILG",
    "response#2": "VGRRIVVSAGGTREAIDPVRYVGNRSSGKMGYAVAEAARDRGAEAVIVSTVSLPVPAGVRVVAVESALQMMEAVHRECADADALIMAGAVADYRPADAADQKIKRDKTGDMSIDLLENPDIIATTPIETKRGRLTKISFAAETQDLIANAKAKLTKKGARFVVANDVSATDAGFGVDNNRVTIL",
    "response#3": "VGRRIVVSAGGTREAIDPVRYVGNRSSGKMGYAVAEAARDRGAEAVIVSTVSLPVPAGVRVVAVESALQMMEAVHRECADADALIMAGAVADYRPADAADQKIKRDKTGDMSIDLLENPDIIATTPIETKRGRLTKISFAAETQDLIANAKAKLTKKGARFVVANDVSATDAGFGVDNNRVTIL"
  },
  {
    "reference": "MDSVSNVSVNEQGKFNDKEEGFSNLKSLKHVSHSETDFEVSNDEDNQLLELGYKPVFKREFSTWATFSFAFSISGLFATVVTTYSYPLISGGAPSAVWCWLIAGAGCMCIALSVAELVSAYPTSGGLYFTCKDLVPARSMPVVAWVVGWLNLLGQAAGVSSTDWSCAQLLLAAVSISTDLKYIPTNQHIVGVMAAVIVFHGLVNSLSTRWLDRITRFYATFHLIVLVVCMICLLAKCPKFNTGKYVFTDVQASSGWHPIGFSFLFGFLSVAWCMTDYDATAHIAEEIENAAVRAPNAIALALSITYVLGWVFNIVLAFTMGTDLDSLINSELGQPVAQIFYNVLGKKGSMAFTILSFIIINFTGITAMQANARTIWAFSRDQALPFSRYWYKINKTTTTPVIAVWLNVVFCIALNLIGLGSIEAIEAIFSVCAIALDWSYVIPIACKLIFGKRLNYKPGPWNLGWASHFVNAYAVCWTAFVSVIFLMPTVRPVTPQNMNYAVVVLAGVLLFSLVYWWSGARKSYIGPRINVDMESEDPFKTE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a specific function within the cell division site, Golgi apparatus, membrane.2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "TTPDSTGLQFVLIGKAKGAWHVIHIDFEGLHEATCKESDMEEWHARVDKDGEPTCIMGHTQTYPRRKKDAKCFLKKEFKLAEVETKDCDCTDQDYECDYNFERNDKGICVSRGPIPIPEGACKEGDRNGKFLGTSGYRKIPGNTCKDTKETKDKYKDVERSC",
    "response#2": "TTPDSTGLQFVLIGKAKGAWHVIHIDFEGLHEATCKESDMEEWHARVDKDGEPTCIMGHTQTYPRRKKDAKCFLKKEFKLAEVETKDCDCTDQDYECDYNFERNDKGICVSRGPIPIPEGACKEGDRNGKFLGTSGYRKIPGNTCKDTKETKDKYKDVERSC",
    "response#3": "TTPDSTGLQFVLIGKAKGAWHVIHIDFEGLHEATCKESDMEEWHARVDKDGEPTCIMGHTQTYPRRKKDAKCFLKKEFKLAEVETKDCDCTDQDYECDYNFERNDKGICVSRGPIPIPEGACKEGDRNGKFLGTSGYRKIPGNTCKDTKETKDKYKDVERSC"
  },
  {
    "reference": "MTNYAIILAAGKGIRMKSDLPKVLHKVSGLTMLEHVFRSVAAIAPEKNVTVVGHKAESVKEVLAGQSGFVLQTEQLGTGHAVMMAETELAGLEGNTLVIAGDTPLITGDSLRNLIDYHVNHKNVATILTAKAEDPFGYGRIIRNNDGEVTKIVEQKDANEYEQKVTEINTGTYVFDNKRLFEALKNITTNNAQGEYYLTDVISIFRENREKVGAYVLRDFNESLGVNDRVALATAESVMRRRINKKHMINGVTFQNPEATYIESDVIIDPDVLLEANVTLKGKTKIGAGSILTNGTCLVDAQLGQHVVITSSTIEESTLADGVTVGPYAHIRPGSILAEKVHVGNFVEVKGSSLGQNTKAGHLTYIGNAEVGSDVNIGAGTITVNYDGQHKFKTMIGNNVFVGSHSTLIAPLEIGDNALTAAGSTISKNVAADSIAIGRSRQVTKEGYAKRLPHHPNNK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have magnesium ion binding within cytoplasm to regulate cell wall organization, UDP-N-acetylglucosamine biosynthetic process.2. The protein should have a high affinity for acetyl-CoA. The designed protein sequence is ",
    "response#1": "MENGVTLMAPETVFLSLDTVIGRDAVVEPNVVFGPGVTVESGARIRAFSHLEGCHVSRGAIIGPFARLRPGAELAEDVHVGNFVEIKNAQVDEGAKINHLSYVGDATIGARTNVGAGTITCNYDGVMKHRTEIGRDVFIGSDTMLVAPVKVGDAAMTATGTVVTRDVEPGALAIARVPL",
    "response#2": "MENGVTLMAPETVFLSLDTVIGRDAVVEPNVVFGPGVTVESGARIRAFSHLEGCHVSRGAIIGPFARLRPGAELAEDVHVGNFVEIKNAQVDEGAKINHLSYVGDATIGARTNVGAGTITCNYDGVMKHRTEIGRDVFIGSDTMLVAPVKVGDAAMTATGTVVTRDVEPGALAIARVPL",
    "response#3": "MENGVTLMAPETVFLSLDTVIGRDAVVEPNVVFGPGVTVESGARIRAFSHLEGCHVSRGAIIGPFARLRPGAELAEDVHVGNFVEIKNAQVDEGAKINHLSYVGDATIGARTNVGAGTITCNYDGVMKHRTEIGRDVFIGSDTMLVAPVKVGDAAMTATGTVVTRDVEPGALAIARVPL"
  },
  {
    "reference": "MGGKWSKSSLVGWHRVRERLRRTPAAAVGVGSAYQDLHRPVAITSSTTAETNGDCAWVEAQEEEDVGFPVRPQVPLRPMTYKAAFDLSYFLKEKGGLEGLIYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGIRYPLTFGWCFKLVPLDPAEVEEANKEENNSLLHPICQHGMEDEDREVLVWRFDSRLAFTHKAKELHPEFYKDC",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have SH3 domain binding, GTP binding to facilitate suppression by virus of host autophagy, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II.2. The protein design must feature the Dileucine internalization motif; necessary for CD4 internalization, Diacidic; necessary for CD4 internalization motif.3. For general function, the protein need meet that Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD. The designed protein sequence is ",
    "response#1": "MGGKWSKRSGAGWPTIRERMRRAEPAADGVGAASRDLERHGALTSSNTAANNAACAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPEKVEEANEGENNCLLHPMSQHGMDDPEKEVLVWKFDSRLAFHHMAREKHPEFYKDC",
    "response#2": "MGGKWSKSSIVGWPAVRERIRRAEPAAEGVGAASQDLDKYGALTSSNTPTNNAACAWLQAQEEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQKRQEILDLWVYNTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPDKVEEANEGENNSLLHPMSLHGMEDPEKEVLEWRFDSRLAFHHVARELHPEYYKNC",
    "response#3": "MGGKWSKRSVPGWPAVRDRMRRAGPAAEGVGAVSQDLAKHGAITSSNTAATNADCAWLEAQEEEGEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLVYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPDQVEKANEGEDNCLLHPMSLHGMDDPEGEVLEWRFDSRLAFHHVARELHPEYYKNC"
  },
  {
    "reference": "MNLSPDEDFARQLDAEDPLRHFRERFHIPLTKDGQPYIYLVGNSLGLMPKTTRAFVDQELDDWAALAVDAHLEGSTPWYSYHETVREPAARLVGAGPHEVVCMNSLTVNLHLLMATFYRPAKSRFKILMEEPAFPSDTYAVKSQIVHHGFAAKEGVVLARAREGEFAARTEDIEATLEKQGDEIALVLLAGVNFFTGQFFDIARVTAAAHKHGCIVGIDLAHAVGNVPLSLHDWDVDFAAWCSYKYLNSGPGAVAGAFVHERHAMNRELPRLAGWWGNDPATRFQMQLLPEFVPVPSADSWAISNPPIFSMAPLRSSFAIFDEAGGMEALRAKSVKLTGYLEFLLDQIGSGAFTVITPRDPQARGCQLSILAHEHPKELFAKLEAAGVKCDFREPNVIRAAPTPLYNTFHEVWRFAQLLAQHH",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively.2. The protein design should be able to enhance the efficiency of tryptophan catabolic process.3. The protein should have a low Km value for H2O + L-kynurenine = anthranilate + H(+) + L-alanine. The designed protein sequence is ",
    "response#1": "VLEEQGDNIAVVLLSGVHYFTGQLFEINKITQAAQAKGCKIGWDLAHAVGNVELNLHDWNVDFAAWCTYKYLK",
    "response#2": "EAIIEVFPRDGEETLRTEDIVAQIKKDGDEIALVLFGGINYYTGQWFDMSSITKAGHAVGAIVGFDLAHAAGNVPVKLHDWGVDFACWCSYKYQNSGPGGISGIFVHEQ",
    "response#3": "VLEEQGDNIAVVLLSGVHYFTGQLFEINKITQAAQAKGCKIGWDLAHAVGNVELNLHDWNVDFAAWCTYKYLT"
  },
  {
    "reference": "MIDAETKSPFGTIEQALEDIRQGKMVIVCDAEDRENEGDLVLAGQFATPEAINFMATHARGLICLALTPDRCDELGLDLMPAKNEPDLKTAFTVSIDAREGVTTGISAHDRAHTIQVAIDSRTRPDDLAGRGHVFPLKAKPGGVLERTGHTEAAVDLARVAGLYPAGVICEILNEDGTMARVPDLEVYAERHGLTMVTVAELIAYRRRADRLVERIVEVALPTAFGDFNVVGFRSLVDDKHHVAMVKGEVAGKDDVLVRVHSECLTGDVFHSLRCDCGQQLEDALMRIEREGEGVLLYLAQEGRGIGLLNKLRAYKLQEEGFDTVDANLKLGLPADLRDYGIGAQILVDLGLGSIRLLTNNPKKIVGLEGYGLKVTDQVPIEHDPNEHNHEYLRAKAQRMGHLLHHQGLALDEEMVHAEQVEDRRREAEDG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be able to bind Mg(2+) ligand in a variety of conditions2. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.3. For general function, the protein need meet that Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.4. The protein should be optimized for activity in Cofactor biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. The protein must have high specificity towards magnesium ion binding, manganese ion binding, GTP binding, zinc ion binding. The designed protein sequence is ",
    "response#1": "SQAEMPTRYGKFRAVTYRELSSGVEHIALTIGDLSSSDQPLVRVHSECLTGEAFGSLRCDCGEQLELALQAIREEGTGCLIYMRGQEGRGIGLGNKIAAYGLQDVGLDTLDANGALGLPADARDYRAAADILKDLGATRVRLLTNNPEKVDALSAAGIDVVSRG",
    "response#2": "SQAEMPTRYGKFRAVTYRELSSGVEHIALTIGDLSSSDQPLVRVHSECLTGEAFGSLRCDCGEQLELALQAIREEGTGCLIYMRGQEGRGIGLGNKIAAYGLQDVGLDTLDANGALGLPADARDYRAAADILKDLGATRVRLLTNNPEKVDALSAAGIDVVSRG",
    "response#3": "RVATARLPTEFGEFRVIGYRNDVDNAEHVALVHGEVEGASKVLVRMHSKCLTGDVFGSRRCDCGAQLHAAMRQVVDAGAGVIVYLDQEGRGIGLLNKLRAYALQDGGDDTVEANERLGFAPDLRNYGIGAQILRDLGLTTVRILTNNPRKLVGVEGYGLEITERVASTRLPTPFATFQVTDYLEHRTGQHTLALVLGDLHAPDAEPPLVRLHSSCLTGDIFGSQRCDCQAQLHAAMQAIADEGRGVLLYLPQEGRGIGLMAKLQAYALQDQGHDTITANERLGYPADARDYTSALAILHTLGAKRVRLLTNNPQKLQALAQGGIEVL"
  },
  {
    "reference": "MLKLLSASQIRSADAHTIQHESIGSLDLMERASNAFVQKFVAFALDRQTNISIYCGTGNNGGDGLAIARILFKNGYKNVSVKILHFSFKTTSDFDENLRRLAKTNISAEIFNEESEFPEEHSPILIDAILGSGLNKPLEGGYARWITHLNNLSKKVIAVDVPTGFFCDAPLDKNAIVFHADHVISFQRPKLNFLLPESAQFIKEFHVVDIGLDEHFIQAQDSNKVWVEENDIKAILKPRASFSHKGTFGHALIIAGQTETMGAALLSSEACLHAGAGLTTAFIPEPGLSALNARSPEVMAILGDLQKIEWNKFDALGIGPGFGVQVSSLEVLRKCLTHYRGPAVYDADALNMLAANKELFNLLPENSIVCPHVKEFDRLFGAHKNWQQRIETMERESTERGLIIILKNRYSIITAPSGVIYFNPTGNPAMASGGSGDVLTGILTALLAQGYSALQTTIAGVYIHGKAADELVQERKMFTVPARYLIKQLPITMGKLINSFA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a binding site that is specific to the target K(+), (6S)-NADPHX, AMP ligand.2. The desired protein should be able to perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate3. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.4. The protein design must be able to perform metal ion binding, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, ATP binding, NADHX epimerase activity in order to contribute to nicotinamide nucleotide metabolic process.5. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.6. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MATVPEGTLMRRAATGLSVVCAGLLPGVYGSRVVLLVGSGDNGGDALYAGARLARRGARVEAILAGSRTHAGGLAALLDAGGRTAGTDVIERADLIVDGLVGIGGAGELREPYRTLALAAGAAPGLVVAVDVPSGVDASTGRVDGEAVRAQLTVTFGACKNGLLVDPGRSFCGPVELVDIGHEDVARLLPRPGAESDKYRRGVLGVAAGSHLYTGAAVLAVGGALRAGAGMVRYAGAEEPVAQVRAHWPEAVITVLDRPSIDEVGRVQAWVLGPGLGTGERAHDLAASVLASDVPVLIDADGLTIVAKDRELLRRTAPVLLTPHAGELARLTGAERADIEAARLEHVRAAAAELGVTVLLKGSTTLIAGPSGQARVNVTGSSWLATGGTGDVLSGVAGALLAQGLSCYDAAACGAYVHGLAGRLAADGAPLAAADVATAVPAGVAAGSHLYTGAAVLAVGGALRAGAGMVRYAGAEEPVAQVRAHWPEAVITVLDRPSIDEVGRVQAWVLGPGLGTGERAHDLAASVLASDVPVLIDADGLTIVAKDRELLRRTAPVLLTPHAGELARLTGAERADIEAARLEHVRAAAAELGVTVLLKGSTTLIAGPSGQARVNVTGSSWLATGGTGDVLSGVAGALLAQGLSCYDAAACGAYVHGLAGRLAADGVVVSGEAWTSGAARLCARGALRAGAGLVTLACPASVLPLQAASYAAIMTREAPDARALAHLLEDARLHTVALGPGLGAGEETRRRVAAAAPGRRLVLDADALTSFAGEADALAALLAGAECAVITPHAGEFARLFAGAEAVLGAPSKLAAARAAARLVGAVVVLKGPDTVVAAPDGRAMIAENAPPFLATAGAGDVLAGFAAGLLAQGMPAFEAAGAAVWLHGEAARE",
    "response#2": "MMKLENAADQSGVTYDQMMENAGKGLAEHLVRIAVEMGVKEILFLCGNGNNAGDCFTAARLLTPQFKVTACLLAGVPKTRTAYAKYKMLKDIAVLEDQEAILEAVRRHRLIADGVFGIGFRGELSPFVQSVFAEIEADSEKKCIAVDIPSGGNGLSGDVAKGTPHCDMTITFGAAKTGLFLTPLSEYCGAIMLVDIGIPAPAFEDVDVAARWPVPGPTDDKYTQGVTGVMAGSDTYPGAAVLCTGAAVAATSGMVRYAGTAAQQVLAQWPEVIATSTFTTTGRVQSWVVGPGLGTDETAAAALCFALEADVPVIVDADALTILAAHPELVDGRSTATVLTPHAGEFARLAGAPPGPDRVADTRRLADRLGVTVLLKGNVTVIAEPGSGAVYLNAAGASWAATAGSGDVLSGVIGALLAAGLPAGEAAAAAAFVHSRAAQLSALDPGPGPAPTSASRILAHLRTAVAG",
    "response#3": "MATVPEGTLMRRAATGLSVVCAGLLPGVYGSRVVLLVGSGDNGGDALYAGARLARRGARVEAILAGSRTHAGGLAALLDAGGRTAGTDVIERADLIVDGLVGIGGAGELREPYRTLALAAGAAPGLVVAVDVPSGVDASTGRVDGEAVRAQLTVTFGACKNGLLVDPGRSFCGPVELVDIGHEDVARLLPRPGAESDKYRRGVLGVAAGSHLYTGAAVLAVGGALRAGAGMVRYAGAEEPVAQVRAHWPEAVITVLDRPSIDEVGRVQAWVLGPGLGTGERAHDLAASVLASDVPVLIDADGLTIVAKDRELLRRTAPVLLTPHAGELARLTGAERADIEAARLEHVRAAAAELGVTVLLKGSTTLIAGPSGQARVNVTGSSWLATGGTGDVLSGVAGALLAQGLSCYDAAACGAYVHGLAGRLAADGAPLAAADVATAVPAALVAGRSAMAGAAILASRACMRAGAGKLTVHTPFYNVSILQTTVPEAVLHIDPNSDCFSTPFDAGGFDALGIGPGIGTSSDTVQAFIEQIRLTKSPIVIDADGLNILGSHRGWIQQLPKKSILTPHKKELFGLISTTRNSFEELERTRELAVHQQIYIIIKGAYSIVVTPEGKCYFNTTGNPGMATAGSGDVLTGIILSLLAQDYSQEDACRLGVYLHGLAGDIAAEELSEEGVMASDIVECITDDDVADLLPRPEAESDKYRRGVLGIVAGSDRFTGAAVLAVGGAIRAGAGLVRFASAETAVRQVRNRWPEAITTVLDGPSVAEVGRVQAWVLGPGMGTGEEAVALARAVLATDVPVLVDADGLTILANDRSLLRRSAPTVLTPHAGELSRLLDVPRERIEAARMEHVRRAVAELGAIVLLKGSTTLIAEQDRPVRVNTTGTPWLATGGTGDVLSGIAGTLLSGGLSGYDAVSCAAHLHGLAAQAASTGISGGHGPIAAMDVATMLPQVI"
  },
  {
    "reference": "MATIQLLNIKKQYDNDIFVINDLNLTVADGEFLVIVGPSGCGKSTLLRIIAGLEKVTSGELYIDNELINNREPADRDIAMVFQNYALYPHMTVRGNLEYGLKNRKTPKEDINKRIAHVAKLLEIEPFLDRKPQQLSGGQRQRVAMGRVIVRKPRVFLFDEPLSNLDAKLRAHMCIEIKKLQRSLKTTSLYVTHDQLEAMTLADKMVVMNKGTIEQIGTPMEIYDCPKTIFVADFIGSPPINFLDRQALEKHLGNLFSCSKDTDILAFRPEAILLGEYPDKGPVFHTQIEFIRPIGTGCHVLTRWNDILFTVDIKERLTSDYGQKLNFTVTHQNFHTFNKKTGNRT",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be optimized for ATP hydrolysis activity.2. The protein must exhibit the following characteristics: Responsible for energy coupling to the transport system.; Part of the ABC transporter complex UgpBAEC involved in sn- glycerol-3-phosphate (G3P) import.3. The designed protein should have a high specificity for ATP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "KCTYGTGSFLIIQAGTQVMRSHHQLLSTVAWSTENSAYYALEGAIFTSGAAIQWLRDGLKIINNAAETETLSRQVTDTKGVYFVPALSGLGAPHWDMNARGAFFGITGGVGREHLVRAVLEAIAYQVKEVVDAINLESTLKVAALKVDGGASQNDFLMQFQADALGVPIERPLILDATAQGAAFGL",
    "response#2": "KCTYGTGSFLIIQAGTQVMRSHHQLLSTVAWSTENSAYYALEGAIFTSGAAIQWLRDGLKIINNAAETETLSRQVTDTKGVYFVPALSGLGAPHWDMNARGAFFGITGGVGREHLVRAVLEAIAYQVKEVVDAINLESTLKVAALKVDGGASQNDFLMQFQADALGVPIERPLILDATAQGAAFGL",
    "response#3": "KCTYGTGSFLIIQAGTQVMRSHHQLLSTVAWSTENSAYYALEGAIFTSGAAIQWLRDGLKIINNAAETETLSRQVTDTKGVYFVPALSGLGAPHWDMNARGAFFGITGGVGREHLVRAVLEAIAYQVKEVVDAINLESTLKVAALKVDGGASQNDFLMQFQADALGVPIERPLILDATAQGAAFGL"
  },
  {
    "reference": "MAETNNECSIKVLCRFRPLNQAEILRGDKFIPIFQGDDSVIIGGKPYVFDRVFPPNTTQEQVYHACAMQIVKDVLAGYNGTIFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYSMDENLEFHIKVSYFEIYLDKIRDLLDVTKTNLSVHEDKNRVPFVRGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFLINIKQENIETEQKLSGKLYLADLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGTKSYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEKTKAQKETIAKLEAELSRWRNGENVPETERLAGEDSALAAEICEETPVNDNSSIVVRIAPEERQKYEEEIRRLYKQLDDKDDEINQQSQLIEKLKQQMLDQEELLVSTRGDNEKVQRELSHLQSENDAAKEEVKEVLQALEELAVNYDQKSQEVEEKSQQNQLLVDELSQKVATMLSLESEPQRLQEVSGHQRKRIAEVLNGLMKDLSEFSVIVGNGEIKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQLLISQHEAKIRSLTEYMQTVELKKRHLEESYDSLSDELAKLQAQETVHEVALKDKEPDTQDAEEVKKALELQMENHREAHHRQLARLRDEINEKQKTIDELKDLDQKLQLELEKLQADYERLKNEENEKSAKLQELTF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that can perform microtubule motor activity, protein-containing complex binding in axon cytoplasm, ciliary rootlet, apical dendrite to support microtubule-based movement, retrograde neuronal dense core vesicle transport, anterograde dendritic transport of neurotransmitter receptor complex, cellular response to ethanol.2. The protein contains novel Kinesin motor domains that confer a unique function or activity.3. The protein must exhibit the following characteristics: Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner; Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL).4. strand-helix-helix-helix-helix-strand-helix-helix-strand-strand-helix is the desired arrangement for the engineered protein.5. The designed protein should have a binding site that is specific to the target ATP ligand.6. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "MASVKVAVRVRPMNRREKDLEAKFIIQMEKSKTTITNLKIPEGGTGDSGRERTKTFTYDFSFYSADTKSPDYVSQEMVFKTLGTDVVKSAFEGYNACVFAYGQTGSGKSYTMMGNSGDSGLIPRICEGLFSRINETTRWDEASFRTEVSYLEIYNERVRDLLRRKSSKTFNLRVREHPKEGPYVEDLSKHLVQNYGDVEELMDAGNINRTTAATGMNDVSSRSHAIFTIKFTQAKFDSEMPCETVSKIHLVDLAGSERADATGATGVRLKEGGNINKSLVTLGNVISALADLSQDAANTLAKKKQVFVPYRDSVLTWLLKDSLGGNSKTIMIATISPADVNYGETLSTLRYANRAKNIINKPTINEDPTAVLLRQYKEEIERLKAELQNCNPSCCEETMMSNKVAPKQCWPHEKNNISGSWDESCVQQLEVEMKKLQQNVQVAVRVRPFNSREKERNAELIVQMNNKSTILTRPSALRANPLAAPTADDEKSFSFDYSYWSYDSNDPHFASQSTVYNDLGKEVLKNAWDGFNCSIFAYGQTGSGKSYSMMGYGEEKGIIPLICEELFQRIQSTPSNSNEQTIYKTTVSYMEIYNEKVKDLLNPNNNKTGGLKVRNNPSTGPYVEDLSKLAVKSFSEIDMLMDEGSKARTVASTNMNATSSRSHAVFTIVFTQSKIDKTRGTAIDRVSKISLVDLAGSERANSTGATGVRLKEGANINKSLSTLGKVISALAENSTSKKAVFVPYRDSVLTYLLKETLGGNSKTIMIAAISPADINYEESLSTLRYADSAKKIKTVAVVN",
    "response#2": "RFRPLSPREIRQGEEVAWYADGDTVVRSEQNPSVAYAYDRVFAPTTTTRQVYDVAAQHVVSGAMEGVNGTIFAYGVTSSGKTHTMHGDQRSPGIIPLAVKDAFSIIQETPNREFLLRVSYLEIYNEVVNDLLNPAGQNLRIREDPQGTFVEGIKEEVVLSPAHALSLIAAGEEHRHVGSTNFNLLSSRSHTIFTLTVESSPCGESNEGEAVTFSQLNLIDLAGSESSRAETTGVRRKEGSYINKSLLTLGTVISKLTDGKATHIPFRDSKLTRLLQSSLSGQGRVSLICTVTPASSNSEETHNTLKFAHRAKRPVEVIGRIRDYPDRKEKSPSILQVNTDNQTVRVRADVGYRDFTLDGVSFSEQEGLEEFYKKFIEERIKGVKVGNKCTIMMYGPTGAGKSHTMFGCGKEPGIVYRSLRDILGDSDQDGVTFVQVTVLEVYNEEIYDLLSTNSSNNLGIGWPKGASTKVRLEVMGKKAKNASFISGTEAGKISKEIVKVEKRRIVKSTLCNERSSRSHCIIILDVPTVGGRLMLVDMAGSENIDQAGQTGFEAKMQTAKINQGNIALKRVVESIANGDSHVPFRDSKLTMLLQDSFEDDKSKILMILCASPDPKEMHKTLCTLEYGAKAKCYVGRDDASTEQDIVLHGLDLESEHCIFENIGGTVTLIPLSGSQCSVNGVQIVEATHLNQGAVILLSEHPVEVIGRIRDYPDRKEKSPSILQVNTDNQTVRVRADVGYRDFTLDGVSFSEQEGLEEFYKKFIEERIKGVKVGNKCTIMMYGPTGAGKSHTMFGCGKEPGIVYRSLRDILGDSDQDGVTFVQVTVLEVYNEEIYDLLSTNSSNNLGIGWPKGASTKVRLEVMGKKAKNASFISGTEAGKISKEIVKVEKRRIVKSTLCNERSSRSHCIIILDVPTVGGRLMLVDMAGSENIDQAGQTGFEAKMQTAKINQGNIALKRVVESIANGDSHVPFRDSKLTMLLQDSFEDDKSKILMILCASPDPKEMHKTLCTLEYGAKAKCIVRGSHTP",
    "response#3": "RVRPFSNREKTENSFPVIYMNGSETIVRNLSTNQVYNFSYDFSFWSFDKDHPNFASQAMIYKTLALPLLERAFEGYNACLFAYGQTGSGKSYTMMGFDEDRGIIPRLCEDLFNQIAQMDKEQVLYHLEMSFFEVYNEKIHDLLVFKAENGQKKQQLRVREHPVLGPYVEGLTVNVVRSYSDIQSWLELGNKQRATAATVMNDKSSRSHSVFTLVMTQTKVEFVNEEKCDRRLTSHINLIDLAGSECCTKAQTTGERLKEGVSINKSLLTLGRVISALSKQSQNGKKTFIPYRESVLTWLLKESLGGNSQTTMIATVSPAGSSTEETLSTLRYAKQACSVRVPQDIQLSGSHILKEHCVFENKDGVVTLVPHKDALVYVNGRKVIEPEVLQTGSRVILSSGSGIITSIRIRPIGKNQGVWSHGKLSNDPYGREYIRQQTSTSSSTIQQEYLFNNVFGMESKNYDIYKRSVKSVVRNVFSGYNGIVFAYGMTGTGKTYSMQGTENEPGIIPLAMNDLFEMVENNSDDDTFQIRISYLEIYNERIRDLIGNSDEEPRIRENASGEVNVTPLTRVLVTSPEEVSQVIEQCNAIRKTAATDFNTYSSRSHAILQVFLIRNNPTAHTSQISSLSLVDLAGSERASAHHERRKEGAFINKSLLTLGTVISRLSAAANPSLTSNSGHIPYRESKLTRLLQQSLSGQSQISLLATISIESNHTMETTNTLKFASRAQNLPQDIRQAEE"
  },
  {
    "reference": "MPQPVPTANRLLDLFSLKGKVVVVTGASGPRGMGIEAARGCAEMGADLAITYSSRKEGAEKNAEELTKEYGVKVKVYKVNQSDYNDVERFVNQVVSDFGKIDAFIANAGATANSGVVDGSASDWDHVIQVDLSGTAYCAKAVGAHFKKQGHGSLVITASMSGHVANYPQEQTSYNVAKAGCIHLARSLANEWRDFARVNSISPGYIDTGLSDFIDEKTQELWRSMIPMGRNGDAKELKGAYVYLVSDASSYTTGADIVIDGGYTTR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The NADP(+) binding site should be located in a region of the protein that is accessible to the ligand.2. The protein design must be able to perform mannitol 2-dehydrogenase (NADP+) activity, L-xylulose reductase (NADP+) activity, NADP binding in order to contribute to glucose metabolic process, xylulose metabolic process, mannitol metabolic process.3. The protein must exhibit the following characteristics: D-mannitol 2-dehydrogenase which is not necessary for D- mannitol catabolism; D-mannitol metabolism occurs via at least two different routes involving mannitol dehydrogenase (MDH) or mannitol 1- phosphate dehydrogenase, and the exact physiological role of mannitol dehydrogenases remains unclear..4. The designed protein is the optimization of the Proton donor, Lowers pKa of active site Tyr to achieve high catalytic efficiency for D-mannitol + NADP(+) = D-fructose + H(+) + NADPH. The designed protein sequence is ",
    "response#1": "MKALHFGAGNIGRGFIGSLLKTSGFELVFTDVNEAVINELNARGEYTVELAAPGQKQEIVGPVTAINSAQDPAALTEAVASADLITTAVGPAVLKIIASSIAEGLKQKNPDHIINIVACENMIGGSSHLKEAVFSHLTEEEQKALSQTVGFPNAAVDRIVPIQHHEDILKVSVEPFFEWVIDETGFIGDVPQITKKIYMLNALHCSLAWFVFKNFSFNKYLYVYQALKNDKVVEFVNNYLNEVILVLNHKYNINLDELNNYKNQIIKRLNSNFIKDDLKRLARNTELKLSKNERILTILDYAKDNNLKHDILLLSYQNGLEYLKEF",
    "response#2": "MKVLHFGAGNIGRGFIGKLLADAGVEVVFADVNQQVLDALNQRHQYQVRVVGEQAQVETVRGVSAVHSGSDDVVALIASVDLVTAAVGPQILERIAPAIARGLVKRHDGGGTQPLNIIACENMVRGTSQLKQHVLNALEPRYHAWVDQHVGFVDSAVDRIVPPVEAGSDDPLAVTVESFSEWILDKTQFKGVPPAHFVATTSMETLVDLEPYIERKLFSVNTGHATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGKLLVAKWNFDEQEHAAYIEKIIQRFQNKYISDAITRVARTPIRKLGAQERFIRPIRELQERNLVSPHLLAMIGIVFNYHDPEDEQSRQLQEMLDQESVDTVIAEVTGIEDPETVKNIKQNVERLIGLGARAIVNQDLEPFIERKLFSVNSGHAAVAYSSAYKGYKTILEGLQDEEILNILKAVQKETRALLLAKWAQYFKQDELIKYHELIISRFSNPEIIDEVSRVARTPIRKLGYDERFIRPIRELNDRKLSYQNHLDIVGKIFAYHDENDAQAIQLMEKALAR",
    "response#3": "MNLIHFGAGNIGCGFIAPILSSIVDHIYFVDNNIDIVNKINTQKLIKIHTSDSKKISITNISAWLLTDFINYKKTWNEVSLLTISIGIKNLKHIIYYVNQLIDYKIKNNQKLIIMCCENGIRVSSLFKSYFSNLNNNIYFVDVLVDRIVSNKNILNDYLECEDYYLWIVDKTQNLMAFVERKLFTLNTGHCITAYLGCLKGHRTIREAIEDPNIHAEVKQAMQESGEVLIRRYGFDHDMHNAYIEKILGRFANPYLVDEVDRVGRQPIRKLGANDRLVKPLLGTIEYGTENQTLLKGIAAALKYTNDTDPQAVELQTSLKEVGVTKTLAKYTGLAEDSDEVAQIETLYNLLT"
  },
  {
    "reference": "MSESATPHGSPGEKLRLAGLIGVLGVVYGDIGTSPLYAVQASLSYFPGNKLQESDVLGLLSLIFWALIITVTIKYVLLIMRADNEGEGGTLSLMALAQRVTQSDRTKWIIGIIGICGAGLFFGDATITPAISVLSAVEGMEVVSPGLKEFVLPIAIAVILVLFFVQRFGTARVGGAFGPIMVIWFVVIGALGLHQIFIHPNVLRALVPVYGAAFIMRHDLLAFIALGSVVLAVTGAEALYADMGHFGAKPIRVSWLFFVLPCLLLNYFGQGALVIRDPHAASNPFFFLLPHALVVPMVILATIATVIASQAVISGAYSVARQSTQLGLLPRMPIRYTNETEQGQIYVPPVNSFLFVVVVLLVLGFGSSSALASAYGIAVTGTFLSTNALAAFVYCRHFNWPLRRTVLVFGAIGLVDFAFFSSNVLKVFDGGWVPLAIGFSLITVMTTWRRGRALLHQRWQQDSLPLASFLGRLPQSRIVRVPGVAVFMTGNPEYTPSSLLHNLKHNKVLHETVVFVTVRNPGVPFVGDARRAKVEELSEGVYRVLLSFGFMESPNIPRALDLLREQGLPFNPMQISYFLGRETIVAATVPKLGFIRRAIFLFMLRNAISATEFFKIPSDRVVELGVRIAI",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein can be expressed in plasma membrane and perform potassium ion transmembrane transporter activity without triggering undesirable cellular responses.2. The protein must exhibit the following characteristics: Transport of potassium into the cell. The designed protein sequence is ",
    "response#1": "IVLFATGRVRMDAVALFVIVAFALSGTLTVPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTVAGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLVVNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFRDLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVNGVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPESELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGMLAKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLMGKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMMLGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMRDLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVNGVSEFRARDVLLIDMSAADVDLRQFCSEQLY",
    "response#2": "IVLFATGRVRMDAVALFVIVAFALSGTLTVPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTVAGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLVVNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFRDLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVNGVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPESELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGMLAKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLMGKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMMLGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVNGVSEFRARDVLLIDMSAADVL",
    "response#3": "IVLFATGRVRMDAVALFVIVAFALSGTLTVPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTVAGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLVVNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFRDLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVNGVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPESELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGMLAKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLMGKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMMLGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVNGVSEFRARDVLLIDMSAADVD"
  },
  {
    "reference": "MEIVSTGNRTVSEFVLLGFHEIPGLHLLFFSVFIILYVSIITGNMLIAVVVVSSQRLHTPMYFFLVNLSFIEVVYTSTVVPKMLEGFVQEATISVAGCLLQFFVFGSLATDECFLLAVMAYDRYLAICHPLRYPLLMGPQWCLGLVLTVWLSGFMVDGLVVALMAQLRFCGPNLIDHFYCDFSPLMILACSDTGVAQVTTFVLSVVFLTVPFGLVLMSYAQIVVTVLRVPSGTRRTKAFSTCSSHLAVVSTFYGTLMVLYIVPSAVHSQLLSKIIALLYTVVTPIFNPVIYTLKNQEVQQALRRLFYCKPTEM",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that localizes to plasma membrane and can perform G protein-coupled receptor activity, olfactory receptor activity with high specificity and efficiency.2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.3. The designed protein should contain one or more G-protein coupled receptors family 1 profile domains that are essential for its function. The designed protein sequence is ",
    "response#1": "WNITLILLIHIDSSLHTPMYFFINQLSLIDLTYISVTVPKMLVNQLAKDKTISVLGCGTQMYFYLQLGGAECCLLAAMAYDRYVAICHPLRYSVLMSHRVCLLLASGCWFVGSVDGFMLTPIAMSFPFCRSHEIQHFFCEVPAVLKLSCSDTSLYKIFMYLCCVIMLLIPVTVISVSYYYIILTIHKMNSVEGRKKAFTTCSSHITVVSLFYGAAIYNYMLPSSYQTPEKDMMSSFFYTILTPVLNPIIY",
    "response#2": "GNIGMILLIKTDSRLQTPMYFFPQHLAFVDICYTSAITPKMLQSFTEENNLITFRGCVIQFLVYATFATSDCYLLAIMAMDCYVAICKPLRYPMIMSQTVYIQLVAGSYIIGSINASVHTGFTFSLSFCKSNKINHFFCDGLPILALSCSNIDINIILDVVFVGFDLMFTELVIIFSYIYIMVTILKMSSTAGRKKSFSTCASHLTAVTIFYGTLSYMYLQPQSNNSQENMKVASIFYGTVIPMLNPLISAVEY",
    "response#3": "GNLGLVAVIYLEPRLHTPMYIFLGNLALMDSCCSCAITPKILENFFSVDRRISLYECMAQFYFLCLAETADCFLLAAMAYDRYVAICNPLQYHSMMSKKLSIQMSIGTFITSNLHSLIHVGCLLRLTFCKSNRIDHFFCDILPLYRLSCTDPFINELMIYIFSMPIQVFTITTVLVSYFCILLTIFKMKSKDGRGKAFSTCASHFFSVSIFYVCLLMYIRPFDEGNKDIPVAVFYTIIIPLLNPFIYSLRNKELCSALRRTL"
  },
  {
    "reference": "MAEKTFINRLQLTDFRNYGSASLRLDGRHVVLTGNNGSGKTNLMEAVSFLSPGRGLRRAVLSDVARAGAASGFSIFASLEGMAGDVELGTGSEVLDETAVRRLRINGASVRSVDELTDHLRVLWLTPAMDGLFTGSSSERRRFLDRLVLSIDPQHGRRASDFERAMRSRNKLLSEGRFDASWLAGIEQQMAALGIAMALARQEMMRLLAALIEQRREPETFPGADLMLSGFMDEHAGTAAIDLEDTYRDSLAGSRGRDAAAGRTLEGPHRSDLLVRHREKDMEAERCSTGEQKALLIGLILAHAELVATMTGFAPILLLDEIAAHLDEGRRAALFDRIDVLGGQAFMTGTDAQMFASLGDRAQFVTVDDGHLSL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design should be able to enhance the efficiency of DNA replication.2. The ATP binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MKFSTLFLGLTGTGKTFVAMYMFNEIQEVKKEDGEYGVVSQAYVNCREVGGTPQAVLSAITQRITGEEVPKHGINLGEYIERIKENLVDKKALIYLDEVDTLIKRRGGDIVLYQLLRANADISVIMVSNDINIRDYMEPRVLSSLGPTIFFKPYV",
    "response#2": "MKFSTLFLGLTGTGKTFVAMYMFNEIQEVKKEDGEYGVVSQAYVNCREVGGTPQAVLSAITQRITGEEVPKHGINLGEYIERIKENLVDKKALIYLDEVDTLIKRRGGDIVLYQLLRANADISVIMVSNDINIRDYMEPRVLSSLGPTIFFKPYR",
    "response#3": "MKFSTLFLGLTGTGKTFVAMYMFNEIQEVKKEDGEYGVVSQAYVNCREVGGTPQAVLSAITQRITGEEVPKHGINLGEYIERIKENLVDKKALIYLDEVDTLIKRRGGDIVLYQLLRANADISVIMVSNDINIRDYMEPRVLSSLGPTIFFKPYR"
  },
  {
    "reference": "MPVLQGPNICSTRGVSSCQQCLAASPVCAWCSDEALPLGSPRCDLKENLLKDNCAPESIEFPVSEARVLEARPLSDKGSGDSSQVTQVSPQRIVLRLRPGRALRNPCYDMKNACLPMFGYKHVLTLTDQVTRFNEEVKKQSVSRNRDAPEGGFDAIMQATVCDVSLEGMECQVWLAQIKMGKEVCECSEEDYRPSQQDECSPREGQPICSQRGECLCGQCVCHSSDFGKITGKYCECDDFSCVRYKGEMCSGHGQCSCGDCLCDSDWTGYYCNCTTRTDTCMSSNGLLCSGRGKCECGSCVCIQPGSYGDTCEKCPTCPDACTFKKECVECKKFDRGILHEENTCNRYCRDEIESVKELSKDSCFIHNRPALCFFPTECPKGPDILVVLLSVMGAILLIGLATLLIWKLLITIHDRKEFAKFEEERARAKWDTANNPLYKEATSTFTNITYRGT",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must have high specificity towards neuregulin binding. The designed protein sequence is ",
    "response#1": "GPWGGPAGDDAFNFKVGSWIKDIIIYSNGAIQSIAFKDGNGHCYGKFGGKDPNDIGVEKKVEIDGNLEHLTSISGTYGNYKGFEVVTSLSFITNVTKHGPFGTASGTSFSIPIEGSLVTGFHGKGGYYLDSIGIQ",
    "response#2": "GPWGGPAGDDAFNFKVGSWIKDIIIYSNGAIQSIAFKDGNGHCYGKFGGKDPNDIGVEKKVEIDGNLEHLTSISGTYGNYKGFEVVTSLSFITNVTKHGPFGTASGTSFSIPIEGSLVTGFHGKGGYYLDSIGIQ",
    "response#3": "GNEWDDGIFHNVKKINVGVNDFDTVFVKFHYSKYNRIEAGAGHGNATTHNPDDEIMIAGGDYIEAVEGTYTESHITSITFRMRKGDMMPQYGRLNGTPFSLRGERGSKAIGFYGRSSGVHLTALGVHFSC"
  },
  {
    "reference": "MNDERVFGITTGTAATAAALASLLCLKGCEASKVRVRTPSGILEVDVEYVERISGDTARACVIKRPYPDPDVTVNLEIISTVQITGTPEIMIRGGEGVGIVTKPGLQVPPGEAAINPVPRRMIEENLREHLRDDEGAVVTVSVPGGERIASRTMNPRLGIIGGISILGTTGIARPMSTEAYRESLACQIDIATARGFRELVFVPGNIGERFAREYFESIEDERIIQMANFPGYMLEEADGRGVERIILMGHAGKLIKLSAGIFQTRSSDADARREIMAAHAALMGASRDTVTRIFNDGTVEEMISELEGEGLTGRVFNSIADAIRERFNERFSFDTDVLIFSLDGRILNSNFKTKRNHRFTEVF",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should take into account the physiological conditions of the cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 6/10, Cofactor biosynthesis, adenosylcobalamin biosynthesis pathway.2. The protein design should aim to mimic or enhance the function of natural proteins involved in cobalamin biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.3. The protein must exhibit the following characteristics: Catalyzes the methylation of C-1 in cobalt-precorrin-5B to form cobalt-precorrin-6A..4. The protein should have a fast turnover rate for Co-precorrin-5B + S-adenosyl-L-methionine = Co-precorrin-6A +   S-adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "MLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITPRGVVAGIGCRRDTPFPLLATLLARQLEAQKLDPLALKAIGSVTLKKGEPGLIQLASCCRVPFKTFTAEALREFEHHFPGSGFVRKTVGVGSVSGPAAWLLSQGQLLGETLREQGVTITKGNI",
    "response#2": "MLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDAYIDLGDVLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITPRGVVAGIGCRRDTPFPLLATLLARQLEAQKLDPLALKAIGSVTLKKGEPGLIQLASCCRVPFKTFTAEALREFEHHFPGSGFVRKTVGVGSVSGPAAWLLSQGQLLGETLREQGVTITLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITPRG",
    "response#3": "MLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITPRGL"
  },
  {
    "reference": "MTDKSKDNSKSLLYCSFCGKNQKEVQKLIAGPKVYICDECIRLCNDIITEETIKQNNITDTENKINYLPKPHEIKKHLDNYVIGQNYTKKVLSVAVYNHYKRLYNFNKKTDSVELGKSNILLIGPTGSGKTLLAQTLAKLLDVPFTITDATTLTEAGYVGEDVENVIQKLLQKCKYNVKKAELGIVYIDEIDKIARKSDNPSITRDVSGEGVQQALLKLIEGTLASIPPQGGRKHPQQEFLQVNTSNILFICAGAFSELSKIVSKRLDAGTEIGFKANIKEKKQKKSEDFLLKQVEPEDLIKFGLIPEFIGRLPIITILNKLTEDALVNILCKPKNALIKQYQTLFELENVKLEFNAESIQLIAKKAMNKNTGARGLRSIIEGILLNIMYELPSMVNIEKILINESVVNSNSLPKIIYGKNKSKKASGE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must be optimized for zinc ion binding, ATP binding.2. For general function, the protein need meet that ATP-dependent specificity component of the Clp protease3. The ATP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "KSNILLIGPTGSGKTLLAETLARLLNVPFTIADATTLTEAGYVGEDVENIIQKLLQKCDYDVEKAQTGIVYIDEIDKISRKADNPSITRDVSGEGVQQALLKLIEGTIASVPPQGGRKHPQQEFLQVDTHNILFIVGGAFAGLDKVIRDRTEKGGIGFSAKVKSKEEKGPVSRTLSGVEPEDLIKYGLIPEFVGRLPH",
    "response#2": "GKSNILLMGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVENIIQKLLQKCDYDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTIASVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVIEQRAHTGTGIGFGTAIKDSSNKSKEGELLKKVEPEDLVKYGLIPEFIGRLPVLTSLEELSEEALIQILREPKNSLTKQYAALFSLEDTELEFREDAR",
    "response#3": "QKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENILLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTVASVPPQGGRKHPHQEFIQIDTTNVLFIVGGAFDGIETIVKNRLGEKTIS"
  },
  {
    "reference": "MLEAAALQGKHIVLGLSGGVACYKAAELTRELTKAGATVQVVMTEAALQFITVATMQALSGRPVYTNQWDGREPNHMPHINLTRQADAVLVAPASADFIAQLAQGRAGELLALLCLARPIERCPLLLAPAMNREMWAHPATQRNVAQVRADGATVLGPAPGDQACGEVGDGRMLEAAELRDELIAFFQPKLLAGRRVLVTAGPTFEALDPVRGLTNLSSGKMGFAIAHAAAEAGAQVTLVAGPVHLPTPRGVQRLNVRSAQQMFDAVLPLAPAHELFIATAAVADWRPAAVAEQKLKKDGSGRVPAFEMTENPDILAAVARLPEAQRPWCVGFAAESEKVLEHARAKRLRKGIPLIVANHGPSTFGQDHNALVLVHEGGETELPAADKLTLARQLVAEVARLAPRRA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should contain one or more DNA/pantothenate metabolism flavoprotein C-terminal domains that are essential for its function.2. The protein must be able to phosphopantothenoylcysteine decarboxylase activity, FMN binding, metal ion binding, phosphopantothenate--cysteine ligase activity.3. The protein requires Mg(2+) to activate a critical catalytic site.4. We require a protein that can specifically bind to the metabolites produced in the Cofactor biosynthesis, coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway.5. The protein have enhanced H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.6. The designed protein should be able to compete with endogenous proteins or inhibitors for CTP binding.7. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A The designed protein sequence is ",
    "response#1": "MNIVVGITGGIAAYKAVGVVRGFVLAGHSVQVIATEHALRFVGKPTLEAISRNTVHTDLYEGVAEVRHVALGQAADLVVIAPTTANTLAKLAAGLADDLLGNTVLATTAPILLAPAMHTEMWRNAATVANAALLALRGYRFIGPATGQLTGTDVGPGRMSEPDEIVAAAETVLRGGRHVVVSAGGTREHLDPVRFLGNSSSGRQGYALARAAVARGARVTLVAANVALPDPAGAGVVRVVSALDLRDAVHAAAADADVVVMTAAVADFRPAKRVDHKIKKEAGVPAPVELEANPDVLAELVATRATRPSGQLLVGFAAETGGPDGDVLTLGRAKLARKPADLLVVNEVGAGLGFEVDHNAAVERVLATAAGGGRHVVVSAGGTREHLDPVRFLGNSSSGRQGYALARAAVARGARVTLVAANVALPDPAGAGVVRVVSALDLRDAVHAAAADADVVVMTAAVADFRPAKRVDHKIKKEAGVPAPVELEANPDVLAELVATRATRPSGQLLVGFAAETGGPDGDVLTLGRAKLARKPADLLVVNEVGAGLGFEVDHNAAVEAVLASPDGSAEGGRHVVVSAGGTREHLDPVRFLGNSSSGRQGYALARAAVARGARVTLVAANVALPDPAGAGVVRVVSALDLRDAVHAAAADADVVVMTAAVADFRPAKRVDHKIKKEAGVPAPVELEANPDVLAELVATRATRPSGQLLVGFAAETGGPDGDVLTLGRAKLARKPADLLVVNEVGAGLGFEVDHNAAVERVLAGAAGPAGGRHVVVSAGGTREHLDPVRFLGNSSSGRQGYALARAAVARGARVTLVAANVALPDPAGAGVVRVVSALDLRDAVHAAAADADVVVMTAAVADFRPAKRVDHKIKKEAGVPAPVELEANPDVLAELVATRATRPSGQLLVGFAAETGGPDGDVLTLGRAKLARKPADLLVVNEVGAGLGFEVDHNAAV",
    "response#2": "MNIVVGITGGIAAYKAVGVVRGFVLAGHSVQVIATEHALRFVGKPTLEAISRNTVHTDLYEGVAEVRHVALGQAADLVVIAPTTANTLAKLAAGLADDLLGNTVLATTAPILLAPAMHTEMWRNAATVANAALLALRGYRFIGPATGQLTGTDVGPGRMSEPDEIVGGRHVVVSAGGTREHLDPVRFLGNSSSGRQGYALARAAVARGARVTLVAANVALPDPAGAGVVRVVSALDLRDAVHAAAADADVVVMTAAVADFRPAKRVDHKIKKEAGVPAPVELEANPDVLAELVATRATRPSGQLLVGFAAETGGPDGDVLTLGRAKLARKPADLLVVNEVGAGLGFEVDHNAAVEPARLRVLVAVTGGVAAIKAPHLLRLLKRAGHEVRVLATPRALEFVTPLSLAVAAGGEVATEEAWFRPDGRALHIDLARFADLVLVAPATADALAKAALGLGDDLLSATLLAGPRRVAWAPAMNEAMWLAPQTQGHVERLKALGHAFFGPGHGPLAAEGEGEGWGRMLEPEELLERVRALLAPPAGPAGGRHVVVSAGGTREHLDPVRFLGNSSSGRQGYALARAAVARGARVTLVAANVALPDPAGAGVVRVVSALDLRDAVHAAAADADVVVMTAAVADFRPAKRVDHKIKKEAGVPAPVELEANPDVLAELVATRATRPSGQLLVGFAAETGGPDGDVLTLGRAKLARKPADLLVVNEVGAGLGFEVDHNAAVEQLVALLAPAAAPAAAAAPAAAAAAAVADVRDAGIKALVTAGPTREHLDPVRYISNPSTGLMGYALARALSERGAEVVLISGPTHLAAPPGVKKVAIISADEMYEEVFKYYNGCRLIIKAAAVSDFRPSSEAEQKVKKHDASLSLELVANRDILKDLGQNKNDRILIGFAAETENATENARDKLLKKNLDLIVINDLTEPGAGFAVTTNRVSI",
    "response#3": "MNIVVGITGGIAAYKAVGVVRGFVLAGHSVQVIATEHALRFVGKPTLEAISRNTVHTDLYEGVAEVRHVALGQAADLVVIAPTTANTLAKLAAGLADDLLGNTVLATTAPILLAPAMHTEMWRNAATVANAALLALRGYRFIGPATGQLTGTDVGPGRMSEPDEIVARLGGLAGRRLVVTAGGTREPIDPVRFLGNRSSGRQGLAIASAAAHAGGSVQVIAANIESALLSALPAGVQVTRVGSALQMRDATIAAVARADALVMTAAVADFRPEVASVSKMKKDPTNEDVPTIRLIRNPDILAEIGHSEQRPPLVVGFAAETGTDEEILAYGADKAARKGADFICLNRVGESVGFGDVPNDIRVLDADGQEVAHAEGSKRVVADAVLDLVRARRPVAR"
  },
  {
    "reference": "MEKSGGRIVMDEIRSFEKAHLFDLGHPLLNRIADSFVKAAGVGALQAVSREAYFTVVDGAGFDSNNVGPPSEITGNKKHRFPNLRGESSKSLDALVKNTGKESLQWGLAAGLYSGITYGMTEVRGGAHDWRNSAVAGALTGAAMAMTTSERTSHEQVVQSALTGAAISTAANLLSSVF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that localizes to chloroplast outer membrane and can perform amino acid transmembrane transporter activity with high specificity and efficiency.2. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "VTGFFFFMWYFLNVIFNILNKKIYNYFPYPYFVSLIHLVVGVVYCLISWSVGLPKRAPINGTLLKLLFPVALCHGIGHITSNVSFAAVAVSFAHTIKALEPFFSAAATQFILGQQVPFSLWLSLAPVVIGVSMASLTELSFNWTGFINAMISNISFTYRSIYSKKAMTDMDSTNVYAYISIIALIVCIPPALIFEGPKLMQHGFSDAIAKVGLTKFVSDLFLVGLFYHLYNQIATNTLERVAPLTHAVGNVLKRVFVIGFSIIVFGNKISTQTGIGTSIAIAGVAMYL",
    "response#2": "VTGFFFFMWYFLNVIFNILNKKIYNYFPYPYFVSLIHLVVGVVYCLISWSVGLPKRAPINGTLLKLLFPVALCHGIGHITSNVSFAAVAVSFAHTIKALEPFFSAAATQFILGQQVPFSLWLSLAPVVIGVSMASLTELSFNWTGFINAMISNISFTYRSIYSKKAMTDMDSTNVYAYISIIALIVCIPPALIFEGPKLMQHGFSDAIAKVGLTKFVSDLFLVGLFYHLYNQIATNTLERVAPLTHAVGNVLKRVFVIGFSIIVFGNKISTQTGIGTSIAIAGVAMYL",
    "response#3": "VTGFFFFMWYFLNVIFNILNKKIYNYFPYPYFVSLIHLVVGVVYCLISWSVGLPKRAPINGTLLKLLFPVALCHGIGHITSNVSFAAVAVSFAHTIKALEPFFSAAATQFILGQQVPFSLWLSLAPVVIGVSMASLTELSFNWTGFINAMISNISFTYRSIYSKKAMTDMDSTNVYAYISIIALIVCIPPALIFEGPKLMQHGFSDAIAKVGLTKFVSDLFLVGLFYHLYNQIATNTLERVAPLTHAVGNVLKRVFVIGFSIIVFGNKISTQTGIGTSIAIAGVAMYAVFA"
  },
  {
    "reference": "MPLNKDIKRVLVIGSGPIVIGQAAEFDYAGTQACRALKQEGLEVILINSNPATIMTDNAMADKIYIEPLTLETVKRVIKKERPDSLLSTLGGQTGLTLSMQLAKEGFLDKYNVKLLGANPETIDKAEDRQMFKDTMESIGQPCIPSKVVNTVEDAVDFANQPGIGYPLIIRPAFTLGGTGGGIVNDEEELKEITRNGLYLSPITQVLVEKCIAGWKEIEFEVMRDAKGNVITVCSMENFDPVGVHTGDSIVIAPAVTLADKEYQMLRSAALKIIDTLKVEGGCNCQFALNPDSFEYAVIEVNPRVSRSSALASKATGYPIAKVAAQIAIGYTLDEIKNAVTGKTYACFEPALDYVVVKLPKWPFDKFVYAKRELGTQMKATGEVMAIGSTFEQAIMKAVRGAEIGHDCLISPKMLDLDDKTIHDRLSDCTDERLFVVYEALRRGVSVDEIHSITKIDEWFLYKLCKLIDMEKTLKNDFNEETYLEAKKIGYTDKVIEKITGKKIEKPVHAVFKMVDTCAAEFAAMTPYFYSTYDNEDEASEFIANRGHDRKTVIVFGSGPIRIGQGIEFDYASVHCVWALKEKGYDVVIVNNNPETVSTDFDTADRLYFEPLTDEDVMNIIRVEKPVGVVVAFGGQTAIKLTKHMAEHGVNILGTPPDAIDAAEDRERFDELLEQLKIKRPQGFTVMTCDEALEVANKIHYPVLMRPSYVLGGQNMIIAFNDDDIKEYMAIILDQGIENPVLIDKYLMGTELEIDAICDGEDILIPGI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein contains novel ATP-grasp domains that confer a unique function or activity.2. The designed protein should have a binding site that is specific to the target Mg(2+), substrate ligand.3. The design of the protein must focus on the precise arrangement of amino acids in the Proton donor, active site to enable effective catalysis of 2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP +   carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate.4. The protein must be engineered to have a high affinity for Mn(2+) in order to increase its catalytic activity.5. We require a protein that can specifically bind to the metabolites produced in the UMP biosynthesis via de novo pathway, Pyrimidine metabolism pathway.6. For general function, the protein need meet that Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L- lysine and an essential component of the bacterial peptidoglycan.7. The designed protein should have metal ion binding, ATP binding, diaminopimelate epimerase activity to facilitate lysine biosynthetic process via diaminopimelate, arginine biosynthetic process. The designed protein sequence is ",
    "response#1": "MAANLILEDGTSYTGKSFGAEIDMAGEVVFQTGMVGYPESLTDPSYSSQILVLTYPLIGNYGIPDPEKDALGLSRWFESSKIHVAALIVGDYTEAYSHWSAKKSLHDWLKEHNIPAIYGIDTRSLTKKIRENGTMLGKVAVDCGIKNNQIRCLCERGASVKVVPWNYKLESSEYDGLFLSNGPGDPKMCAETIANVKRVIKEEDNKPVFGICLGHQLLSSAAGCKTYKLKYGNRGHNQPCTHTSSKRCFITTQNHGFAVDVETMPKDWSILFTNENDKSNEGIVHNSKPFFSVQFHPEHMAGPRDLELLFDVFKNAVKLYSDRKRFDDLLEGLGIKRPKGYGVLTKEEALDAAEKLGYPVLLRPSYVLGGQNMIIAFSPEDVKEYMDIILRQKQDNPVLIDKYLAGLEIEVDAISDGVDVLIPGIMEHIERTGIHSGDSIAVYPAPNVDDQICVVDCGLKYNQLRCLLKRGARVDIVPWNYDIKTAKYDGLFLSNGPGDPNMCQVIIENIREVFKQKRPIFGICLGHQLLSIAAGCVTYKMQYGNRGHNQPATHSITGRCYMTSQNHGFCVDAAQLPSDWEILFTNANDNSNEGIVHSVLPYFSVQFHPEHTAGPEDLECLFDVFKEISSVDRKRFDDLLEGLGIKRPKGYGVLTKEEALDAAEKLGYPVLLRPSYVLGGQNMIIAFSPEDVKEYMDIILRQKQDNPVLIDKYLAGLEIEVDAISDGVDVLIPGIMEHIERTGIHSGDSIAVYPAPNVDDQ",
    "response#2": "MAANLILEDGTSYTGKSFGAEIDMAGEVVFQTGMVGYPESLTDPSYSSQILVLTYPLIGNYGIPDPEKDALGLSRWFESSKIHVAALIVGDYTEAYSHWSAKKSLHDWLKEHNIPAIYGIDTRSLTKKIRENGTMLGKVAVDCGIKNNQIRCLCERGASVKVVPWNYKLESSEYDGLFLSNGPGDPKMCAETIANVKRVIKEEDNKPVFGICLGHQLLSSAAGCKTYKLKYGNRGHNQPCTHTSSKRCFITTQNHGFAVDVETMPKDWSILFTNENDKSNEGIVHNSKPFFSVQFHPEHMAGPRDLELLFDVFSDIVNDQAVDCGIKNNQIRCLCERGASVKVVPWNYKLESSEYDGLFLSNGPGDPKMCAETIANVKRVIKEEDNKPVFGICLGHQLLSSAAGCKTYKLKYGNRGHNQPCTHTSSKRCFITTQNHGFAVDVETMPKDWSILFTNENDKSNEGIVHNSKPFFSVQFHPEHMAGPRDLELLFDVFEALNGTTKARVSQCTDQRLFAIYQALKRGIMNVDDIHSVTMIDEWFLNKLMNLVSMEKTFELVKYGKEELTTQLYLKAKKSGYPDKVIENICAIDCGLKLNQVRCFVSRGARLDVVPWNARPDPAEYDGLFLSNGPGDPETCREVVDNLRALLLPTDGPVKPIFGICLGHQILSTAAGCRTFKMRYGNRGHNLPCMHLGTRRCYMTSQNHGFAVDSSEMPAGWEELFTNLNDGTNEGIVHRTRPYFSVQFHPEHTAGPTDLEVLFDVF",
    "response#3": "MAANLILEDGTSYTGKSFGAEIDMAGEVVFQTGMVGYPESLTDPSYSSQILVLTYPLIGNYGIPDPEKDALGLSRWFESSKIHVAALIVGDYTEAYSHWSAKKSLHDWLKEHNIPAIYGIDTRSLTKKIRENGTMLGKVLLGKPGDKLNIAGRQADQVASIGGRVAPSGAVYSVEEAIITAKRLGYPILIRAAYALGGLGSGFAHDETELRKIVSKALLHSNQVLLDKSLKGWKEIEYEIIRDSYDNCIAICNMENLDPLGIHTGESIVVAPSQTLTDSEYYLLRSMSIKIVRHLGVIGECNVQFALNPKSEEFYIIEVNPRLSRSSALASKATGYPLAYVAAKLSGAGDVDCIIIEVNARGEVVDCGLKYNQLRCLLKRGARVDIVPWNYDIKTAKYDGLFLSNGPGDPNMCQVIIENIREVFKQKRPIFGICLGHQLLSIAAGCVTYKMQYGNRGHNQPATHSITGRCYMTSQNHGFCVDAAQLPSDWEILFTNANDNSNEGIVHSVLPYFSVQFHPEHTAGPEDLECLFDVFLDYFSKKGQEAVDCGIKNNQIRCLCERGASVKVVPWNYKLESSEYDGLFLSNGPGDPKMCAETIANVKRVIKEEDNKPVFGICLGHQLLSSAAGCKTYKLKYGNRGHNQPCTHTSSKRCFITTQNHGFAVDVETMPKDWSILFTNENDKSNEGIVHNSKPFFSVQFHPEHMAGPRDLELLFDVFKKKLKGEPEL"
  },
  {
    "reference": "MTKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTMLKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFITAKMKKVNNFTTGQIYESVIRKERRGEYLGKTVQVIPHITNEIQEFIHRGAAGADVALVEIGGTVGDIESLPFLEAARQLSLRAGRNAAAFVHLTLVPYVAAAGELKTKPTQHSVQKLREIGIFPDALLCRADRPIPDDERAKISLFANVAEEAVISVWDADTIYKIPQMLHDQGLDKIVCDKLGLSPKPADLAMWDKLIYALENPKEEVTIGMVGKYVDLTESYKSLTEALRHAGIHTESRVNIEYVDSEEIEAHGTKSLEKYDAILVPGGFGKRGVEGKILAARYARENKIPYLGICLGMQVALIEYARNKAGLSMANSTEFDAETEQPVVALINEWQNHDGKVETRDENSDLGGTMRLGAQTCAVRSGTLASEIYGNVVTERHRHRYEANNHYLGRVEAAGLIVSARTPTEDLCEIMELSRDVHPWYLGVQYHPEFKSTPRDGHPLFISFARAAIDHKKAQGNKA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should consider the endogenous proteins and enzymes present in the CTP from UDP: step 2/2, Pyrimidine metabolism pathway to avoid interference with native metabolic processes.2. The protein design must be focused on enhancing the efficiency of glutamine metabolic process.3. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates. The designed protein sequence is ",
    "response#1": "LRDAYLSVSEALSHAAIFHGCEVDIRWVASHELYSYEDAQAALKGVSGIIVPSGFGERGAEGMVFAARYARENDIPFFAEGMGMQMAVVEYARNVCGLEADSTEITPTVKNPVIDLLAELKELDGDTMRLGKYSCRLKEDTKIRKIYGKEVIEERQRHRYELNPEYQSVLEQDGMTLSGIEEKHSLVDCIELPSHPWFIGVQFNAEFKSRPNRPHPLYVGFVV",
    "response#2": "LPDAYMSVTEALHHGGIEHDAQVKITWINAEELEEPNADLDEIFVGCKGILVPGGFGDRGVEGKIRAIQYAREHEIPFLGLCLGMQCAVIEFARHVAGLTNAHSTEFVPETPHPVIALMEDQQDVEEKGGTMRLGAYPCLLADGSHSRAEYGAGEISERHRHRFEFNNAYRAQLEEKGMVIAGTSPDDRLVEVVEIADHPWFVASQFHPELKSRPNHPHPLFAGFVY",
    "response#3": "LRDAYLSVSEALSHAAIFHGCEVDIRWVASHELYSYEDAQAALKGVSGIIVPSGFGERGAEGMVFAARYARENDIPFFAEGMGMQMAVVEYARNVCGLEADSTEITPTVKNPVIDLLAELKELDGDTMRLGKYSCRLKEDTKIRKIYGKEVIEERQRHRYELNPEYQSVLEQDGMTLSGIEEKHSLVDCIELPSHPWFIGVQFNAEFKSRPNRPHPLYVGFVV"
  },
  {
    "reference": "MKTARTIVVGITGGIAAYKAVNIVRAFVLNGDSVHVVATPAALRFIGKPTLEAISRHPVYSELYEGVAEVRHVAIGQSADLIVVAPATANTIAKLAAGLADDLLGNTILASTAPLVIAPAMHTEMWNHAATVANIATLRSRGVTVVGPGVGRLTGHDVGAGRMEEPDTIVAVAMAALEGTPDAGQPEQDLVNRRILISAGGTREPLDPVRFLGNRSSGKQGVALAEAARARGGLVTLVACNLEVPAPDGVHVVQAGSARDLQEALSASAASADVIIMAAAVADYRPTDVRSEKIKKEHTGDTLTLELVKNPDILAELSAACRPGQVIVGFAAETESDPDRLLTLGREKVTRKGCDYLVVNRVGWSEGFATDRNTVLLLDRHGVIVKEASGSKMSVAHRILDVVVQTRLP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. The protein should be optimized for activity in coenzyme A biosynthesis, Cofactor biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The protein have enhanced H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. A protein with FMN binding, phosphopantothenoylcysteine decarboxylase activity, phosphopantothenate--cysteine ligase activity is needed to enhance coenzyme A biosynthetic process in a specific context.5. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..6. The designed protein have Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MSGGIAAYKACHLVRDLKEAGDDVRVVPTPSALNFVGAATFEALSGHPVSTTVFDAVDEVQHVRVGQEADAVVIAPATADVMARLVAGRADDLLTATVLVAQCPVIVVPAMHTEMWWNPATQANVRTLRERGVIVLEPAHGRLTGRDTGPGRLPEPDQIAEAAAALLDSHDLAGQRVVITAGPTREPLDPVRYISNHSSGKMGYALARAAVEAGASVQLVSGPVALAVPDRVHCHPITTAREMLDVCMDLVENCDIFIGCAAVADYRPMVESEQKIKKSNEEMSLQLTRNPDILASVAGHSAKPFSVGFAAETEKLREHALGKLDRKGLNMIVANDVSDSSIGFNSENNAALVLWEGGERELPMMSKEELADRLLDRVRTLHAR",
    "response#2": "MSGGIAAYKACHLVRDLKEAGDDVRVVPTPSALNFVGAATFEALSGHPVSTTVFDAVDEVQHVRVGQEADAVVIAPATADVMARLVAGRADDLLTATVLVAQCPVIVVPAMHTEMWWNPATQANVRTLRERGVIVLEPAHGRLTGRDTGPGRLPEPDQIAELAAALHRPDALAGRKAVVTAGPTFEPIDPVRGLTNRSSGKQGYAIAAALVELGAEVTLVSGPTALGAPVGVTRIDAESALEMQAATQNALPADIAVLSAAVADWRVDTIAGGKIKKGPGGPPTLALIENPDILAGIAAPGPNRPALVIGFAAETTDLEANARAKLSRKGCDWIIGNDVSDDVFGSDGNAVLITGDGAESWEQMPKEQVAERLARRIADALA",
    "response#3": "MSGGIAAYKACHLVRDLKEAGDDVRVVPTPSALNFVGAATFEALSGHPVSTTVFDAVDEVQHVRVGQEADAVVIAPATADVMARLVAGRADDLLTATVLVAQCPVIVVPAMHTEMWWNPATQANVRTLRERGVIVLEPAHGRLTGRDTGPGRLPEPDQIAEIAALLLADPTLLAGRRVLVTAGPTREAIDPVRFVSNRSSGRMGYALAEVAWRRGAAVTLVSGPTALPPPVGVELVRVETAAAMAEAVAAHLPAADVLVMAAAPADFRPAAPAAAKLTKEDGLPPPLAWALTADILQSTRPLRRPDTRVVGFALETADDALARARAKLEAKGLDLVVLNRADEADAGFDTETNRVTVLYRNGTAEQIELLPKTEIADAILDRLAELL"
  },
  {
    "reference": "MSVRIVVLAAGQGTRMKSKLPKVLHPVAGAPLVGWVVDAAAGAEPDEIIAVVGYGADQVAAVLPDGVRAVQQAEQLGTGHAALMAIDAIGDVEGDIVVVLPGDAPLVTSDTLSRLIAGHREREPAVTVLTTRLADPTGYGRIVRDDAGEVVGIVEERDADETQRRIDEVGVSMYAFAGGELRRALNEVGTSNDQGEMYLTDVIGILAQEGAPIAAVEAPPMEVAGVNSHDQLADVGRVLRSRINQDWMQAGVSMLDPERVYIDPAVELDPGAVVFPGVHLRGTTRVAAGAQVGPDVFAEDSVIGPDSRVWYSVLRGAEVGANAEVGPFASLRPGTVLGEGTKAGTFVEMKNTSLAAGAKVPHLSYMGDATIGEASNVGAGSITCNYDGFQKHATVIGDRVRIGSDTMLVAPVSIGDDAWTGAGSTITRDVPAGSLAVERAVQREIPDYAARREAKESGE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that can modulate the overall flux of metabolites through the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway, enhancing or suppressing its activity as needed.2. The designed protein should have a binding site that is specific to the target UDP-N-acetyl-alpha-D-glucosamine ligand. The designed protein sequence is ",
    "response#1": "SGVTIIDPENTYIDVDVEIGSDTVIEPGVYLQGQTKIGESCMITTGSKVVDSQIDANVTIHSSTIENSVMESHTDAGPYAHLRPQSHIKKYAHIGNFVEIKKAVIGEHTKVGHLTYVGDATLEDHINIGCGVTFVNYDGVQKFHTTVGSHSFIGSSSNIIAPVNIADHAFVAAGSTITKDVPKHAMGIARQKR",
    "response#2": "VVILAAGQGTRMKSATPKVLHQIGGLPLIGHVLRTASALAPERAVVVVRHERDLVADAVTGLLPGVDVVDQDEIPGTGRAVELALAALDGFDGDVLVLSADVPLVEVSVVEGLLARHRAAAACVTMLPDDPGRYGRLICQGSQLLRIVEYKDATDEERSITLCNSGVIAAEAKLLFSLLAEVKNDNAAGEYYLTDIVALARQEGLTASAVLAPPEETLGINTRAELARAEALFQAQKRAEALENGVTLIAPDTVFFAYDTVIGRDAVIEPNVVFGPDVTVESGATIRAFSHLEGCHVSRGAIVGPYARLRPGAELAEGAKVGNFVEVKNAMIHEGAKVNHLSYIGDASIGARSNIGAGTITCNYDGFMKHRTEIGEGAFIGSNTMLVAPVTIGNEAMTATGSVINRDVPDGALALARARQENKPGKARALFEALRAKKQAKK",
    "response#3": "IILAAGQGTRMKSKRYKVLHEILGRSMVDHVLTQVEKLDPQNIVTVVGHGADEVKKALGDRTQYVLQAEQLGTGHAVMQAAELLKDKTGSTIVANGDTPLLTAETFEKLFDSHQQLHAKATVLTAKAPNPSGYGRVIRNEDNEVIKIVEQKDASEAEKRIDEINTGVYCFDNQALFDALSQVTNDNAQNEYYITDVIEILKSQDEVISAYQDALGVNDRVALSQAEEIMKQRINTQHMENGVTIADPSATWIDAGVEVGEDTTLLPGVHLLGTTAVGRDCRIGPNAVLRDTRVGERCTIGGSTLEGATLADDVRVGPYCHIRPGSTIEAGARLGNYAEVKASRVGAGSRVGHFSYLGDADVGEDVNIGAGTITANYDGTEKHRTVIGDGAFIGSDSMLVAPLRIGAGARTGVGSVVTRDVPPGASAILGIPAKVLRK"
  },
  {
    "reference": "MKILVINCGSSSLKYQLIESKDGNVLAKGLAERIGINDSLLTHNANGEKIKIKKDMKDHKDAIKLVLDALVNSDYGVIKDMSEIDAVGHRVVHGGEYFTSSVLITDDVLKAITDCIELAPLHNPANIEGIKACKQIMPDVPMVAVFDTAFHQTMPDYAYLYPIPYEYYTKYKIRKYGFHGTSHKYVSQRAAEILNKPIESLKIITCHLGNGSSIAAVKNGKSIDTSMGFTPLEGLAMGTRSGSIDPSIISYLMEKENISAEEVVNILNKKSGVYGISGISSDFRDLEDAAFKNGDKRAQLALNVFAYRVKKTIGSYAAAMGGVDVIVFTAGIGENGPEIREFILDGLEFLGFKLDKEKNKVRGEEAIISTADSKVNVMVVPTNEEYMIAKDTEKIVESLK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be able to bind substrate ligand in a variety of conditions2. The protein design should consider the endogenous proteins and enzymes present in the Metabolic intermediate biosynthesis, acetyl-CoA from acetate: step 1/2 pathway to avoid interference with native metabolic processes.3. A protein that can perform magnesium ion binding in cytoplasm to support organic acid metabolic process, phosphorylation, acetyl-CoA biosynthetic process.4. The Proton donor/acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.5. The protein must be engineered to have a high affinity for Mn(2+) in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "MNKILFATSNKGKLAEFEAMLAPSGLSLIPQQDIGCDADVVEDGKTFAENALLKARHAARATGLPALADDSGLVVDALGGAPGIYSARFAGEPKSDAANNAKLVRELADVPDAHRAAHFRCVLVYMRHADDPAPIICEGSWEGRILTEPRGENGFGYDPLFWIPERQCTAAELSADAKNRISHRGKALAAMRKQLEACG",
    "response#2": "MTTIAINAGSSSLKFALFDDTGRVLARGLVERIGEEGSRLRLRMGDQEEPFETGLPDHAAAMDWMLRRLGDGQGLGELGGLGAVGHRIVHGGERYTAPTLVDDEVIERLEALAPLAPLHNPANLAGIREALRAFPHVPQVAVFDTAFHATLPPHAYRYALPAALAERWGVRRYGFHGTSHRYVAERAARLLGRPLEGLRLVLAHLGGGCSVTAVSGGRSVDTSMGFTPLEGLIMGTRSGDVDPGVIAYLMRQAGLTLEEINTLLNKESGLLGLSGLSSDMRDIERAARDGNRRAVLAYEIFAYRVRKYIGAYAAAMGGLDALVFTAGVGENSAPIRARVCEGLEFLGVRLDPARNEARLGGPARISRPESPVRVLVIPTDEELAIARETLAVLR",
    "response#3": "MLVLAHLGGGCSVTAVSGFYFGFAA"
  },
  {
    "reference": "MQRQGLCSGVPQLKYLLNGLVSVPEHDDMPIVGLALDSREVVCGDAFVALSGAKQHGLVHVEQAINNGACVVLFDPVGNGQQLAEDLQNSITMHIPMIAVEALGLKLGELAARFYGDPSRFMTVIGITGTNGKTSCSQFLSQMLDDCGIIGTLGWGEWGKLNKTLNTTPDALAIQKILAELLKNKKRAVAMEVSSHGLEQGRVNGVMFKGAVFTNISRDHLDYHGTMDAYVQAKLALLTTPGLVFAAVNLDDGYSERIIAACPKSVQLWQFSVKGKIADSGESVIAENIQHKTDGIEFDVRWRDELQQLKVPLFGDFNIENVLAVLTVMLAMKVSLDEATKKLTAIKQVDGRMERFGAETDPMIFVDYAHTPDALDKVLSSVRKHCKQALWVVFGCGGNRDTGKRSQMGEIAEHWADHVVITDDNPRYENGLDIVNDILAGCRSAKVEVIQNREQAIQKVVTNAAKNDCIVIAGKGHEQYQESNGVCIPFSDRQIVMDALDRRKIHCERSE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should be able to enhance the efficiency of cell wall organization, cell division.2. The protein design should consider the endogenous proteins and enzymes present in the peptidoglycan biosynthesis, Cell wall biogenesis pathway to avoid interference with native metabolic processes.3. The designed protein must have at least one Mur ligase central domain.4. The protein design must feature the Meso-diaminopimelate recognition motif motif. The designed protein sequence is ",
    "response#1": "VTGTNGKTTTATLLYELVRLAGEKAGLLSTVCNYIGEEKIPATHTTPDALSIQELLRRMVDAGCRYCFMEASSHAIHQKRIAGLDFDGAIFSNITHDHLDYHKTFKAYIEAKKAFFDRLPAHAFALTNADDKNGLVMLQNTRAHRYTYSCRTMADFTAKVLEKHLDGTLLRLDGEEVWTKFTGDFNAYNLLAVYAAL",
    "response#2": "VTGTNGKTSVASFTRQIWEALGLEAVNIGTTGVEGAREAPLAHTTPEPITLHRLLAGLRADGVTHAAMEASSHGLAQRRLDGVSLVAGAFTNLSQDHLDYHPTLEAYFDAKAGLFTRVLPQDATAVIDTDDPRGPEMADRARARGLRVLTVGREPSADLRIGAQRFGAEGQTLRFEWTGAAYQVDLGLIGGFQAGNVLIAAGLE",
    "response#3": "VTGTNGKTTTAFLLEAMLRSVGRTCVLIGTIETHVGDEVRVSPHTTPESRDVLEIFADGVKAGATEAVMEMSSHALEQERVWGLPVDVAMFTNLTQDHLDYHGTMEKYFAAKARLFEGVGSPAPRVAVINGDDSYGRRLIAANRQSQMMGYGLEPLSGDYRAEDIKLQAGETRFIFKTPNGDALMRSSLTGRVNVYNLLAASCAAG"
  },
  {
    "reference": "MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFCETWPQLDAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIVSVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAFTFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIVLPEPETPPAATVVALEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein have Sigma-54 factor interaction, GAF domains with specific sequence motifs that are crucial for its activity.2. The protein must exhibit the following characteristics: Also activates expression of hyf operon (encodes the silent hydrogenase-4 gene cluster)..3. The designed protein should be involved in phosphorelay signal transduction system, positive regulation of DNA-templated transcription initiation, DNA-templated transcription by localizing to transcription regulator complex and exhibiting identical protein binding, cis-regulatory region sequence-specific DNA binding, ATP binding.4. The designed protein should have a binding site that is specific to the target ATP ligand. The designed protein sequence is ",
    "response#1": "DVSIHVPYLEDTAQESLMQWYHKGLNAFEQTCPSGHILFNEFSESLLNVLISQDKEVLEQVLSDTQTRYAELKSVMEQGRDKLLEINSHGGERANKLVNALAERDEDTNLIGSVIRLWDIIGVEQEDSGENAIVLRPSEHMMFPTYPGLPEDGITVTFDREML",
    "response#2": "MVGKSPAMQHLLSEIALVAPSEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQIISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIT",
    "response#3": "PRVGLIPHQQWIAHEVGRRFAPRVLLADEVGLGKTIEAGLIIHQQLLTGRAERILVIVPDTLRHQWLVEMLRRFNLKFSVFDEDRCVEAYADSDNPFYTEQLVICSLELLRKKRRLDQALAADWDLMVVDEAHHLEWTEDAPSRAYRIVEALSEEIPGVLLLTATPDQLGHQSHFARLRLLDPDRFYDYEAEVSVDWLIDFLKENRREKVLIIASQAETALSLEEALRTREGIQATVFHEGMSIIERDKAGAYFAQETGGAQALICSEIGSEGRNFQFASQLILFDLPLNPDLLEQRIGRLDRISDLNVLISGETGTGKELVAKAVHQGSPRAANPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLRQEVVEGRFRADLYHRLSL"
  },
  {
    "reference": "MIPMHVAVIMDGNGRWARKQLQERTFGHEQGVSVLESIVDECINCGIRFLTVYAFSTENWSRPPTEVSFLFELLSAAIQRVRTTYRERNVKVQFCGERTTQIPETVIAAMNCIEQDTAACTGLILSVCFNYGGHTEIAQACRSVLADCLEGDAVENIKTRLQMPIEQFIQQIDTHLYANLPPVDLLIRTGCEKRLSNFLPWHLAYAEIIFSDLLWPEFSVRAFKDCLDEFASRTRRFGGVQLSPMTGVYSDTHPHSSTNALSNHD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should take into account the physiological conditions of the Lipid metabolism pathway. The designed protein sequence is ",
    "response#1": "GNAVATILATLGTDVADPVQRLAAVTASTRAAKAQLRSMDKDAILAYSAALMAPYGVQLASTLSGVKPPWPYTFNLCVSNVPGPEDVLYLRGSRMEASYPVSLVAHSQALNVTLQSYAGTLNFGFIGCRDTLPHLQRLAVYTGEALDQC",
    "response#2": "LSETLKEQSWFSTPRSAWEKVKAVRQVERPSASDYISHIFDFFVESHGDRAFSDDKAMIGGLAFLDGQPVTIIADEKGKDFKECQERNFGMPMPEGYRKALRLMKQAEKFNRPVISFVNTSGAFCGIEAEERGQGEAIARNLMEMSSLKVPVLCLMIGEGGSGGALATAVGNEVWMMENATYSILSPEGFASILWKDANRAKEASEVMNITAQDLKRLGVIEEIIPEFGGADHKTTEAIAGYMKEHIKDFLREV",
    "response#3": "GNAVATILATLGTDVADPVQRLAAVTASTRAAKAQLRSMDKDAILAYSAALMAPYGVQLASTLSGVKPPWPYTFNLCVSNVPGPEDVLYLRGSRMEASYPVSLVAHSQALNVTLQSYAGTLNFGFIGCRDTLPHLQRLAVYTGEALDQC"
  },
  {
    "reference": "MWNSGFESYGSSSYGGAGGYTQSPGGFGSPAPSQAEKKSRARAQHIVPCTISQLLSATLVDEVFRIGNVEISQVTIVGIIRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Also plays a role in base excision repair (BER) probably through interaction with UNG.2. The engineered protein is expected to adopt a strand-helix-strand-strand-strand-strand-strand-strand-strand-strand-strand-helix-helix-turn-helix-based conformation.3. A protein with OB capability for targeted gene regulation.4. A protein that can perform enzyme binding in nucleus, chromatin to support mitotic G1 DNA damage checkpoint signaling. The designed protein sequence is ",
    "response#1": "IRILISSDPHVGYGEKDPVRGNDSFVSFNEILEIARERDVDMILLGGDIFHDNKPSRKALYQALRSLRLNCLGDKPCELELLSDTSLTTGDTAVCNINYLDPNINVAIPVFSIHGNHDDPSGDGRYSALDILQVTGLVNYFGRVPENDNIVVSPILLQKGFTKLALYGISNVRDERLYHSFRENKVKFLRPDLYRDEWFNLLTVHQNHSAHTPTSYLPESFIQDFYDFVLWGHEHECSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQGRRSCSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQS",
    "response#2": "MLSTGTKSLDSLLGGGFAPGVLTQVYGPYASGKTTLALQTGLLSGKKVAYVDTEGGFSPERLVQMAETRGLNPEEALSRFILFTPSDFKEQRRVIGSLKKTVDSNFALVVVDSITAHYRAEENRSGLIAELSRQLQVLLWIARKHNIPVIVINQL",
    "response#3": "MLSTGTKSLDSLLGGGFAPGVLTQVYGPYASGKTTLALQTGLLSGKKVAYVDTEGGFSPERLVQMAETRGLNPEEALSRFILFTPSDFKEQRRVIGSLKKTVDSNFALVVVDSITAHYRAEENRSGLIAELSRQLQVLLWIARKHNIPVIVINQP"
  },
  {
    "reference": "MVYNTSCLILPLALIILDFTLISSLAHPIPSTLNVLSYGAKPDGSKDSTKAFLAAWDVACASANPTTIIVPKGRFLVGNLVFHGNECKQAPISIRIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIYGGILDAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFHIVIDNSNNVNIDGVKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVFIQTIRCGPGHGISIGSLGRAEEEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFARNIVFQHINMKMVKNPIIIDQHYCLHKPCPKQESGVKVSNVRYEDIHGTSNTEVAVLLDCSKEKPCTGIVMDDVNLVSVHRPAQASCDNANGSANDVVPFTPCLKREIIIT",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must be able to polygalacturonase activity.2. Design the protein with a signal peptide incorporated.3. The protein's Proton donor must be carefully engineered to ensure that the amino acids involved in (1,4-alpha-D-galacturonosyl)n+m + H2O = (1,4-alpha-D-   galacturonosyl)n + (1,4-alpha-D-galacturonosyl)m. are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "DMVITLTQELIMNSFKTIDGRGVNVAIAGGACITIQYVTNIIIHGINVHDCRRTGNAMVRSSPSHYGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLIDAIMGSTAITISNNYMTHHNEVMLMGHSDSYTRDKLMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRFLAPGNC",
    "response#2": "SMVIRLKQELIINNDKTIDGRGANVQIAGGAQLTVQFVHNVIIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNIWIDHVSLARCSDGLIDVILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNHFGRGLVQRMPRCRYGFVHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIV",
    "response#3": "EELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGVNIHDCKRKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGSTAITISNNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWQMYAIGGSAAPTINSQGNRR"
  },
  {
    "reference": "MTERLPHERGFHVSWDQLHRDARALAWRLQSEAPDDTGWRAIVAITRGGMAPAMIIARELDIRTVDTISVKSYDHQNQSEAVVIKAPDMNLIGDGDGVLIIDDLVDTGKTLEIVRKQMPKAHVATVYAKPMGREMVDSFITEVSQDTWIFFPWDLALQYVEPYRGQG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Purine salvage pathway enzyme that catalyzes the transfer of the ribosyl-5-phosphate group from 5-phospho-alpha-D-ribose 1- diphosphate (PRPP) to the N9 position of the 6-oxopurines guanine and xanthine to form the corresponding ribonucleotides GMP (guanosine 5'- monophosphate) and XMP (xanthosine 5'-monophosphate), with the release of PPi2. The guanine, 5-phospho-alpha-D-ribose 1-diphosphate, Mg(2+), GMP binding site should be located in a region of the protein that is accessible to the ligand.3. The protein should be designed to have high stability and activity towards diphosphate + XMP = 5-phospho-alpha-D-ribose 1-diphosphate +   xanthine.4. A protein that can modulate the overall flux of metabolites through the Purine metabolism, XMP from xanthine: step 1/1 pathway, enhancing or suppressing its activity as needed.5. The designed protein should contain one or more Phosphoribosyltransferase domains that are essential for its function.6. The designed protein should have xanthine phosphoribosyltransferase activity, guanine phosphoribosyltransferase activity, hypoxanthine phosphoribosyltransferase activity, magnesium ion binding within plasma membrane to regulate GMP salvage, XMP salvage. The designed protein sequence is ",
    "response#1": "MSWDQLHRDVRELCHQLVARDFKGIIAITRGGLIPAALIARELNVRLIDTVCIKSYDHMEQNQLDILKGVDHDGESWLLVDDLVDTGKTARAVREMLPKAHFVTIYAKPEGKPLVDQYLTEVGQQCWIQFPWDMGVAAKIIE",
    "response#2": "MSWDQLHRDARALAWRLDGQGPDNGGWRAIVAITRGGLVPAMVLSRELDIRVVDTISVQSYLAYQDRSEARVSKAPQADLMGDGTGILIVDDLVDTGKTLELVRKLYPKAHFATVYAKPKGRPMVDTYVTEVSQDTWIFFPWDMALQVLAEEERFYNSRRQRGGWATGTGPFEAIVCITRGGLVPAAIVARELNVRIIETVCIASYHDYKNQGDIKVMKGVGPEITSMRGQGKGVLIVDDLVDTGKTARVVREILPAAHFATVYAKPMGAKQADWEPAEMLESWDQFHRDARALAWRLADKGPFEAIVCITRGGLVPAAIVARELELKLVETVCVASYHDYLHQGDIEVLKTIAPSIAAIGNGRGKGVLLIDDLTDTGKTAKLVREMLPDAHFATVYAKPAGAPMCHTFITEVSQDTWIFFPWDLDLQR",
    "response#3": "MIISWDQFHRDTRALTWRLHEAGPFEAIVCITRGGLVPAAIVARELNVRIIETVCIASYHDYKNQGDIKVMKGVGPEITSMRGQGKGVLIVDDLVDTGKTARVVREILPAAHFATVYAKPMGRPLVDTFITEVSQDTWIYFPWDTGLPVELIQDEYREGSSWDQLHRDVRELCHQLVARDFKGIIAITRGGLIPAALIARELNVRLIDTVCIKSYDHMEQNQLDILKGVDHDGESWLLVDDLVDTGKTARAVREMLPKAHFVTIYAKPEGKPLVDQYLTEVGQQCWIQFPWDMGVAVIQTDEEYYE"
  },
  {
    "reference": "MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITNTLNTRYGTGAAVAFDKIDKAPEERERGITISTSHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLNEYEFPGDDTPIVRGSALQALNDPAGPWGDKIVELFEHIDTYIPEPTRAIDKSFLMPVEDVFSITGRGTVATGRVERGIIKVQDEVELVGLQEDSRKIVVTGVEMFRKLLDQAQAGDNVGLLLRGIQRTEIQRGQVLCKPGTIKPHTKFKAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGATKLPDGMEMVMPGDNVTMEIDLIHPIAIEEGLRFAIREGGRTVGSGVVASIIE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have a high specificity for GTP binding, with minimal off-target effects.2. The protein can be targeted to cytoplasm and perform translation elongation factor activity, GTPase activity.3. The protein must exhibit the following characteristics: This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis..4. The presence of tr-type G domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "PRAPVVTVMGHVDHGKTSLLDAIRESNVVDGEFKGITQHIGAYQITLNGDKKITFIDTPGHEAFAAMRAHGTNVTDIVVLVVAADDGIMPQTIESINHVKAANVAMIVAVNKIDKHDADLDRITNALLQHGVVAESLGGDVIVVPVSAKEKINLDQLKSSILLIADLLELP",
    "response#2": "KPHVNIGTIGHVDHGKTTLTAAITKVMAAAGRAEFRAYDQIDGAPEERERGITISTAHVEYETEARHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLARQVGVPALVVFLNKVDQVDDEELLELVEMEVRELLSSYDFPGDDIPVIKGSALKALEGEESEMGVDAINRLMDAVDAYIS",
    "response#3": "KPHVNIGTIGHVDHGKTTLTAAITKVMAAAGRAEFRAYDQIDGAPEERERGITISTAHVEYETEARHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLARQVGVPALVVFLNKVDQVDDEELLELVEMEVRELLSSYDFPGDDIPVIKGSALKALEGEESEMGVDAINRLMDAVDAYIPEPE"
  },
  {
    "reference": "MKRTIILMLDSFGIGAADDADKFGDVGSDTFGHIAQMCAEGKANNGREGELKLPNLSRLGLAHAAKEATGAFAPGFGDNVDIIGAYGHCQELSSGKDTPSGHWEMAGVPVLFEWGYFSEHQNSFPKELTDKILERAGLTEFLGNCHASGTTILEELGEEHMKTGKPIFYTSADSVFQIACHEESFGLENLYRLCEIAREELEPYNIGRVIARPFVGTGPSDFARTGNRRDYAVEPPSKTVLDKLKQAGGEVVSVGKISDIYAHCGITKKVKASGLEDLFDATLEQIKQAGDNTIVFTNFVDFDSHYGHRRDVSGYAKALEYFDARLPELMALLEEGDLLLLTADHGCDPTWQGTDHTREFVPVLAYGAGLEAGSLGKRGSFADIGQSIASYFGLAAMEYGESFMPAR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Ideal protein should have a binding affinity for key enzymes in the Metabolic intermediate biosynthesis pathway to enhance the overall efficiency of the pathway.2. The protein design should be able to enhance the efficiency of deoxyribonucleotide catabolic process.3. The protein must bind to Mn(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "LILSGGPGKTGAARLAARGALRIGAGLTTIACPRNALSEIAAQITALMVRSLDGPPGLTELLEDARLNALCLGPGLGLGADTQALVLAALKAKRGTVLDADALTRFERNPEVLFDALHENCVLTPHAGEFKRLFPDIAEKLAEPATTGPAFSKVDATRQAAARAGCTVLFKGPDTVIADPDGRCAINSSAYDRAAPWLATAGSGDVLAGFITGLLARGFAPMQAAETAAWLHTECALY",
    "response#2": "GDHKYSHGHALILSGGPGKTGAARLAARGALRIGAGLTTIACPRNALSEIAAQITALMVRSLDGPPGLTELLEDARLNALCLGPGLGLGADTQALVLAALKAKRGTVLDADALTRFERNPEVLFDALHENCVLTPHAGEFKRLFPDIAEKLAEPATTGPAFSKVDATRQAAARAGCTVLFKGPDTVIADPDGRCAINSSAYDRAAPWLATAGSGDVLAGFITGLLARGFAPMQAAETAAWLHTECALSFGPGLIAEDLPEELN",
    "response#3": "AAPVFIDYIRELNLKNLTIVSPDTGGIKSARAVAQRLNASLAAIDKRRMTGKDIQVMNIMGEVDGCDLVIVDDIVSTAGSLTEAIKALRANGANSIYAAITHPILAGPAIERISGL"
  },
  {
    "reference": "MSTNPKPQRKTKRNTNRRPQDVKFPGGGQIVGGVYLLPRRGPRLGVRATRKTSERSQPRGRRQPIPKARQPEGRAWAQPGYPWPLYGNEGLGWAGWLLSPRGSRPNWGPTDPRRRSRNLGKVIDTLTCGFADLMGYIPLVGAPLGGVARALAHGVRVLEDGVNYATGNLPGCSFSIFLLALLSCLTIPVSAYEVRNVSGVYHVTNDCSNSSIVYEAADVIMHTPGCVPCVREGNFSRCWVALTPTLAARNSSVPTTTIRRHVDLLVGAAVLCSAMYVGDLCGSVFLVSQLFTFSPRRYETVQDCNCSLYPGHVSGHRMAWDMMMNWSPTAALVASQLLRIPQAIVDMVAGAHWGVLAGLAYYSMVANWAKVLVVMLLFAGVDGGTTITGGRVAHTTSAFAGIFSVGPKQKIQLVNTNGSWHINRTALNCNDSLNTGFIAALFYAHKFNSTGCPERMASCRPIDKFAQGWGPITYVKPNISDQRPYCWHYAPRPCGIVPASEVCGPVYCFTPSPVVVGTTDPSGTPTYSFGANKTDVLLLNNTRPPSGNWFGCTWMNGTGFTKTCGGPPCDIGGAGNNTLICPTDCFRKHPEATYTKCGSGPWLTPRCLVHYPYRLWHYPCTVNFTIFKVRMYVGGVEHRLEAACNWTRGERCDVEDRDRSELSPLLLSTTEWQILPCSFTTLPALSYIPLVGAPLGGVARALAHGVRVLEDGVNYATGNLPGCSFSIFLLALLSCLTIPVSAYEVRNVSGVYHVTNDCSNSSIVYEAA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must have at least one RdRp catalytic domain.2. The protein must have high specificity towards RNA-dependent RNA polymerase activity. The designed protein sequence is ",
    "response#1": "MYIAKEGYCYLNIFLAMLINVNENEAKAFTKMVRDVIVPMLGIWPTMQDLATACFIMTAFYPETSSAELPRILVDHLDQTMHVIDSFGSLTTGFHVLKAGTAAQLIDFASADLTGEMKLYRVN",
    "response#2": "MSDIFNSPQNKASILNALMKSTQGDVEDVLIPKRFRPAKDPLDSPQAAAAFLKEHKYRILRPRAIPTMVEIETDAALPRLAAMVDDGKLKEMVNVPEGTTAFYPKYYPFHRPDHDDVGTFGAPDITLLKQLTFFLLENDFPTGPETLRQVREAIATLQYGSGSYSGQLNRLLAMKGVATGRNPNKTPLAVGYTNEQMARLMEQTLPINPPKNEDPDLRWAPSWLIQYTGDASTDKSYLPHVTAKSSAGLPYIGKTKGDTTAEALVLADSFIRDLGKAATSADPGAAAKKVLSDFWYLSCGLLFPKGERYTQKDWDLKTRNIWSAPYPTHLLLSMVSSPVMDESKLNITNTQTPSLYGFSPFHGGINRIMTIIREHLDQEQDLVMIYADNIYILQDNTWYSIDLEKGEANCTPQHMQAMMYYRLTREWTNEDGSPRYNPTWATFAMYVGPSMVVDSTCLLMNLQLKTTGQGSGNAFTFLNNHLMSTIVVAEWHKAGRPNPMSKEFMDLEAKTGINFKIERELR",
    "response#3": "MYIAKEGYCYLNIFLAMLINVNENEAKAFTKMVRDVIVPMLGIWPTMQDLATACFIMTAFYPETSSAELPRILVDHLDQTMHVIDSFGSLTTGFHVLKAGTAAQLIDFASADLTGEMKLYRVR"
  },
  {
    "reference": "MGNWLLPESLADVLPAEARRIEELRRELLDLYRTYGFELVAPPLVEYIDSLLSGTGTDLNLRTCKLVDQLSGRTLGVRADMTPQVTRIDAHLLNRAGVTRLCYCGTVLHARPADLLSSRELLQIGAEIYGHAGFEADLEILSLVLDTVAAAGLRQPRLDLCHPGVVRAILDADPAAAAYAEDIVLLLREKDVPGLAELARRDGGIRADTAAALQRLPGLYGGPDVLQQARHDLPLLPGVAAALDALQAIIDAMPDVTLGVDLADIGGYGYHSGVTFALYAEGWHDALVSGGRYDDVSRAFGRARPATGFSLDLRKLARGLPPAGRARAVRAPWGQDAALAAAVRQLRRAGEIVVQALPGHQQSQDEFICDRELVLQDGAWTLRTL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Required for the first step of histidine biosynthesis. The designed protein sequence is ",
    "response#1": "MLAYMNSEALEKTIDTGYTWFYSRSRAALWNKGETSGNTQKVVSVTADCDNDTLLVRVKSNGPACHTGNRTCFK",
    "response#2": "MLAYMNSEALEKTIDTGYTWFYSRSRAALWNKGETSGNTQKVVSVTADCDNDTLLVRVKSNGPACHTGNRTCFL",
    "response#3": "MLAYMNSEALEKTIDTGYTWFYSRSRAALWNKGETSGNTQKVVSVTADCDNDTLLVRVKSNGPACHTGNRTCFY"
  },
  {
    "reference": "MSVLNGKKILLGVSGGIAAYKTASLVRLFIKAGAEIQVIMTPASKDFVTPLTLSTLSKNPVHSSFFNQEDENEKWNNHVELGLWADLMIIAPATANTMSKMANGTCDNLLIAAYLSAKCPIYFAPAMDLDMYKHPSTLASFSLLKQFGNSIIPAESGELASGLSGEGRMAEPENIIAFLEADLESKLPLKGKKFLITAGPTYEAIDPVRFIGNHSSGKMGFDIASEAANLGASVILVAGPTNCKVTNTSVKVISVTSAQEMYDACHEYFSDVDVAIAAAAVADYRPKIVASQKIKKASEDFTIELEKTKDILLSLGEIKKKQFLIGFALETENEIENAKLKIQKKNLDLIVLNSLQDKGAGFGGGTNKVTFIDRAFAIEPMELKSKEAVAIDILNKVIKHFHE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The presence of Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains is critical for the interaction of the designed protein with its intended targets.2. The protein should be optimized for activity in Cofactor biosynthesis, coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The protein must have high specificity towards metal ion binding, phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity, FMN binding.4. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..5. We are looking for a protein with broad substrate specificity for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.6. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "MSGGIAAYKACHLVRDLKEAGDDVRVVPTPSALNFVGAATFEALSGHPVSTTVFDAVDEVQHVRVGQEADAVVIAPATADVMARLVAGRADDLLTATVLVAQCPVIVVPAMHTEMWWNPATQANVRTLRERGVIVLEPAHGRLTGRDTGPGRLPEPDQIAEATAVLADPDALLPVRAGRAVAVSAGSTWESWDDIRFLANRSTGRMGRELALAAWLRGARVTLIAGPTVPEPPTLPNLIFIRVESTCDLLAALKGTDFDTLIMAAAPADFRPAHRVTGKVKKKVNPPALTLATNPDILKTLPRWVGEVVVGFAAEDDDLVNRARAKLADKNLDLVVANQAGGPGGAFGSPNNHIRLLTPDGRHADLPDLTKLEVADALWDAIKAF",
    "response#2": "MSGGIAAYKACHLVRDLKEAGDDVRVVPTPSALNFVGAATFEALSGHPVSTTVFDAVDEVQHVRVGQEADAVVIAPATADVMARLVAGRADDLLTATVLVAQCPVIVVPAMHTEMWWNPATQANVRTLRERGVIVLEPAHGRLTGRDTGPGRLPEPDQIAEAALRLLGERGLSGRVVTITAGPTREPLDPVRYLSNHSSGKMGFALAAAAARHGAEVHLISGPVTLPTPVGVTRHDITTAQEMSDTARQLAKESDVFIGCAAVADYRAANVATHKIKKQANRDGMTLSLVKNPDIIADVGGMTEHRPFVVGFAAETRNLEHHARDKLERKGLDMIVANDVSLEGLGFGADQNAALLFWPQGEKRLPLAPKEALADQLLDLIAERLASAS",
    "response#3": "MSGGIAAYKACHLVRDLKEAGDDVRVVPTPSALNFVGAATFEALSGHPVSTTVFDAVDEVQHVRVGQEADAVVIAPATADVMARLVAGRADDLLTATVLVAQCPVIVVPAMHTEMWWNPATQANVRTLRERGVIVLEPAHGRLTGRDTGPGRLPEPDQIAELAAAVLDDPSLSGRVVTITAGPTREPLDPVRYLSNHSSGKMGFALAAAAARHGAEVHLISGPVTLPTPVGVTRHDITTAQEMSDTARQLAKESDVFIGCAAVADYRAANVATHKIKKQANRDGMTLSLVKNPDIIADVGGMTEHRPFVVGFAAETRNLEHHARDKLERKGLDMIVANDVSLEGLGFGADQNAALVLWRDGRVEHLPRMTKDALAHALVDEVVRLREAP"
  },
  {
    "reference": "FADTLFQLKFTAKQLEKLAKKAEKDSKAEQAKVKKALQQKNVECARVYAENAIRKKNEGVNWLRMASRVDAVASKVQTAVTMKGVTKNMAQVTKALDRALSTMDLQKVSAVMDRFEQQVQNLDVHTSVMEDSMSSATTLTTPQEQVDSLILQIAEENGLEVLDQLSQLPEGASAVGESSVRSQEDQLSRRWAGRRNPAPCVRGGGGHQAAHDRRGGRQLGRPGRGGGCCCGASEGPVVVYRLAALRN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Design the protein with a signal peptide incorporated.2. The designed protein should have protein domain specific binding, protein homodimerization activity within midbody, condensed nuclear chromosome, kinetochore, nuclear matrix, microtubule organizing center to regulate endosome to lysosome transport, plasma membrane repair, viral budding via host ESCRT complex, autophagosome maturation. The designed protein sequence is ",
    "response#1": "ALSQRGPSSVPYEESNKWLIRQHLLNLISSYPSLEPKTASFMHNDGRSVNLLQADGTIPMPFHGVTYNIPVIIWLLESYPRHPPCVYVNPTADMIIKRPHAHVTPSGLVSLPYLQNWVYPSSNLVDLVSDLSAAFARDPPLYSRR",
    "response#2": "ALSQRGPSSVPYEESNKWLIRQHLLNLISSYPSLEPKTASFMHNDGRSVNLLQADGTIPMPFHGVTYNIPVIIWLLESYPRHPPCVYVNPTADMIIKRPHAHVTPSGLVSLPYLQNWVYPSSNLVDLVSDLSAAFARDPPLYSRR",
    "response#3": "ALSQRGPSSVPYEESNKWLIRQHLLNLISSYPSLEPKTASFMHNDGRSVNLLQADGTIPMPFHGVTYNIPVIIWLLESYPRHPPCVYVNPTADMIIKRPHAHVTPSGLVSLPYLQNWVYPSSNLVDLVSDLSAAFARDPPLYSRR"
  },
  {
    "reference": "MSAKRTKFIFTTGGVVSSIGKGLASASIGALLEARGLRVTFVKLDPYINVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTNARLTRANNLTTGKIYQAVISKERRGEYLGATVQVIPHITDEIKARVREATAGADVAIVEIGGTVGDIESLPFLEAVRQLKLEAGANNAVSVHVTLVPWIGAAGELKTKPTQHSVKEMREIGIQPDVLLCRTDRPLSPSIKEKISLFSNVPTDFVISALDVNCLYELPAALHAERLDDLLCERLNIWSRLPDMAPWQRVVERFTRPTKGTVRIAVVGKYVHLRDSYKSLNEALIHAGIANDCRVDLDFVDSEALESSPEEVSRALDHCDAILVPGGFGERGVEGKISAIRYAREKGIPFFGICLGMQLAVVEFARHVAGIAGANSAEFDPQAPSVIDLMPEQRGVRDKGATMRLGAYSCVLSPGSVAAEAYGATEISERHRHRYEFNNDFRDVLTARGLVLSGTSPDKKLVEMIELGGHPYFVGCQFHPELKSRPMAPHPLFVRFVRAGLERAASVATKARDERPSASPASVVH",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The CTP, ATP binding site should be located in a region of the protein that is accessible to the ligand.2. The protein contains novel Glutamine amidotransferase domains that confer a unique function or activity.3. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.4. The designed protein must be optimized for metal ion binding.5. The protein design should consider the endogenous proteins and enzymes present in the CTP from UDP: step 2/2 pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MLDTYASLVEALKAACWQNGYDLELHWIDAEKIEKKDKEAWDALKNAKGVVVPGGFGVRGTEGKIDAIEFARKNNIPYLGLCLGMQLAAIEFARNVAGVNQATSEEFDEDSKACVIHIMDDQKTITEKGGTMRLGGWDCVLDPKSRIAKAYGTKKIRERHRHRYEFNNDFREKLESKGMMIAGTTPDGRLVEVVEIPSHKWFVAVQFHPEFTSRPLTGHPLFNAFIES",
    "response#2": "VLKDAYKSLIEALVHGGIANNVKVNLDWIESETFEKEDPSPYLEGVAGILVPGGFGERGAEGKIAAAHFARTRNVPYFGICFGMQMAVLEAARNLAGIGKASSTEFGPAEEPVVGLMTEWTRGNEKEVRVAGDDLGGTMRLGAYPARLKAGSKIAEIYGTTTISERHRHRYEVNIGYRDQLEACGLNFAGTSPDGVLPETVEIDDHPWFIGVQYHPELKSRPFEPHPLFASFIAR",
    "response#3": "MLDTYASLVEALKAACWQNGYDLELHWIDAEKIEKKDKEAWDALKNAKGVVVPGGFGVRGTEGKIDAIEFARKNNIPYLGLCLGMQLAAIEFARNVAGVNQATSEEFDEDSKACVIHIMDDQKTITEKGGTMRLGGWDCVLDPKSRIAKAYGTKKIRERHRHRYEFNNDFREKLESKGMMIAGTTPDGRLVEVVEIPSHKWFVAVQFHPEFTSRPLTGHPLFNAFIESAL"
  },
  {
    "reference": "MSSKIRPSADDKKLQRVLYFFLERVRAAKDVSGQLLSPLIDNASVDTASVSPSSNGRPTTLKSIQSKIDEFQYHDFSEFVSDLAYLFINVKALYEGTQTYSFVQALEEFCIQQLRTFQQQGYIPVITWPNTDSPSATTSSPISRNPEYSVSPPNGSKFVKNEDEAYDSDLYVEEEDSDVKGRSMVGRDGRYKSEDLKRRKLQPSSKPLSSLEARAKVIMRQVRRYRDGSGRQLFAPFERLPDPRMFPEYYQAIEQPMALEVIQKKLSKHRYETIEQFVDDFNLMFDNAKSFNDPSSQVYRDADFLKNYLADVLRLEAGKLDSEFFNYETDSRASPQLPKNDIQPAVSIDGTLLNVGDWVLIRNPADSSKPIVSQIYRIWKSDDDINYVTVCWYLRPEQTVHRADAVFYENEVFKTSLYRDHPVSEIVGRCFVMYITRYIRGRPKGIRSTPVFVCESRYNDDTKQFSKIKSWKACMPQEVSGSEYEMILFDRPITLTKVASPLLHLLASKSQGLPSPATTDSNTHMLPSQGSLLPPSSISETKSFSTKASTPLSTDDIATPLSSAPNPPSVMPTYARKTSSHSERSSHSSYHNSSHVPTAAFNSPIMRTSTKSTSPIPARPFYAQSGSLQSLNTTQHSHQISGGHSGRMNVPYAKLSYTSHNGRHGGSNGNISGAKTPMTNYTINSMPSLPVFPPAFIVPGTHQKLDESSPVPGIDDVTVINTETAKMLDKDEHQNVLWYTVPPLDPIPLENRNGSLTHSVEYVLYKKS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.2. The protein should be optimized for stability and solubility when expressed in RSC-type complex, nucleus where it will be involved in double-strand break repair via nonhomologous end joining.3. The designed protein should contain one or more Bromo domains that are essential for its function.4. The protein must exhibit the following characteristics: Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning; Controls particularly membrane and organelle development genes.. The designed protein sequence is ",
    "response#1": "SAFICSPPQLPLPNPLRMYLPCPSLNSTEVSVITLAPQHSFLNIVINLNPALALKSYTIITLLNGKRLGAGVSTPPSTLYALEGIKHTEEPIRTVYEAKLSVGLNCLEFVLGC",
    "response#2": "SAFICSPPQLPLPNPLRMYLPCPSLNSTEVSVITLAPQHSFLNIVINLNPALALKSYTIITLLNGKRLGAGVSTPPSTLYALEGIKHTEEPIRTVYEAKLSVGLNCLEFVLGC",
    "response#3": "SAFICSPPQLPLPNPLRMYLPCPSLNSTEVSVITLAPQHSFLNIVINLNPALALKSYTIITLLNGKRLGAGVSTPPSTLYALEGIKHTEEPIRTVYEAKLSVGLNCLEFVLGQ"
  },
  {
    "reference": "MKNKSQEEIVEMFNNIAKTYDLANRILSVGIDKTWRKKACNKAFFLYGKKHINKIVDVACGTGDLMIDWKNTAAKNAIRLDEIVGIDPSVGMMEVGKTKIEDGIFIEAGAESMPLESESTDIISISYGIRNVVKRKEAWSEFSRVLKPHGLAVILEFTRNDDNNLPAKITKFYMNNMMPHIGGLISGSKDAYTYLPESIENFITTEQMKAELQEAGLEPIFVQSYSMKMSTTIIARKIG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein with S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity, demethylmenaquinone methyltransferase activity is needed to enhance ubiquinone biosynthetic process in a specific context.2. The protein must exhibit the following characteristics: Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2).. The designed protein sequence is ",
    "response#1": "MRNLKTFLLTIFFLSLSGCAVLSGGTAPKQG",
    "response#2": "MPIGVPKVPFRNPGEEDPSWVDVYRQLYRERLLFLGQEVDSEISNQLIGLMIYLSIEDDTKDLYLFINSPGGWVIPGIAIYDTMQFVPPDVH",
    "response#3": "MGSTMEMLKVPH"
  },
  {
    "reference": "MNKFKFFIVFLILSLVFLQNEYAFGSSLNEELSYYSVEYDNAKTFKESIKQKNIELTYKIPELHTAQIKTSKSKLNSLIKSNKNVKFVNPTCSTCVVEKSVKTGKNLNNKKNGSHDLFDRQWDMRKITNEGKSYKLSPDRKKAKVALVDSGVNSSHTDLKSINKIVNEVPKNGFRGSENDESGNKNFEEDKLNHGTLVAGQIGANGNLKGVNPGVEMNVYRVFGSKKSEMLWVSKGIIDAANDDNDVINVSLGNYLIKDNQNKKKLRDDEKVDYDALQKAINYAQKKGSIVVAAVGNDGINVKKVKEINKKRNLNSKTSKKVYDSPANLNNVMTVGSIDDNDYISEFSNYGNNFIDLMTIGGSYKLLDKYGKDAWLEKGYMQKQSVLSTSSNGRYIYQSGTSLAAPKVSGALALEIDKYQLKDQPETAIELFKKKGIEKEKYMDKKHYGNGKLDVYKLLKE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must have at least one Peptidase S8 domain. The designed protein sequence is ",
    "response#1": "LYIAKEGYCYLNIFLAMLVNVNENDAKDFTKKVRDVIVPMLKEWPTMEDLASACYLLSVFFPETRSAELPRILVDHSGKTFHVMDSFGSLNTGYHILKASTVSQLISFASNDITGEMKHYNVC",
    "response#2": "LYIAKEGYCYLNIFLAMLVNVNENDAKDFTKKVRDVIVPMLKEWPTMEDLASACYLLSVFFPETRSAELPRILVDHSGKTFHVMDSFGSLNTGYHILKASTVSQLISFASNDITGEMKHYNVC",
    "response#3": "LYIAKEGYCYLNIFLAMLVNVNENDAKDFTKKVRDVIVPMLKEWPTMEDLASACYLLSVFFPETRSAELPRILVDHSGKTFHVMDSFGSLNTGYHILKASTVSQLISFASNDITGEMKHYNVC"
  },
  {
    "reference": "MTTVAVVGATGQVGRVMRDILLERKFPADQVRFFSSPRSAGKQLEFGEQTVEVEDLTQQTEESLRGIDIALFSAGGATSKQWAPVFAAAGATVVDNSSAWRKDPEVPLIVSEVNPAEAAKAQGIIANPNCTTMAAMPVMSALHKAKGLNTLRVASYQAVSGSGLAGVETLARQTAEIGDHNVELAHDGSLLAPEDLGPYVAPIAFNAVPLAGSIVDDGSLETDEEQKLRNESRKILGIPELRVSATCVRIPVFTGHTMVIHAEFDQSITPQEAEDLLRDAPGVSLVDVPTPLNAAGKDDSLVGRIRQDQAVADNKGLVLVVSGDNLRKGAALNTIQIAELLV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein with protein dimerization activity, aspartate-semialdehyde dehydrogenase activity functions is required to enable lysine biosynthetic process via diaminopimelate.2. The protein should have a high affinity for phosphate. The designed protein sequence is ",
    "response#1": "MKVLKFGGTSLANAEKFLCVANIIEKNIITDQIAVVLSAPAKITNHLVNIAENIDKHDKMLEKINLTENIFIELINNISNLQKNFLYKETKKIIQKEFNQLRNIIHGITLLQQCPDNIHAIIVSRGEILSVLIMQSILQAKSYDITIIDPVKSFISCSHGYLDATINIDDSKKYISKLKIAQNTIILMSGFIAGNQNQELIVLGRNGSDYSAAILAVCLNAQCCEIWTDVDGVLTGDPNIISNPYLLQYISYQEAIELSYFGAKVLHPRTIIPISQFNIPCWIKNTNNIQSKGTLICNTHDSDRYIVKGVTHLNYIAMLNISENYMQDLRNITPRIFSTILREKIKIFLITQSYSENKINLFISEKDVSKILYLFNEEFKLELKNKLLKSFKIQKNLSILSVIGSNIHRQYDILSKIFSTLNKSKINIFAISQGASENSISLVINQENILQAVQNIHCQIFSHKKIIHVFLIGIGGIGSTLIKQILKQREYLNKKNIVIKICAIANSKKILYFDNTISLSNWKNNFQQYAKTFNMKILNHLIKKNNLSNSVIIDCTSDQVLSEKYVDFLHNNFHVVTSNKKANTSQLHYYKKIRKAITTTNNKFFYETNVGAGLPVIETIKNLFQTGDTLIRFKGILSGSLSYIFGRLEEGSLLSQATKEAKKLGFTEPNPCDDLSGIDVARKLLILAREFGYDIELKDIKIEPLLPEIFENNQDIDEFLLNLQKLDLIFSRKINTALKRNNVLRFVATIEDPNQFFIKIEEVNIND",
    "response#2": "MKVLKFGGTSLANAEKFLCVANIIEKNIITDQIAVVLSAPAKITNHLVNIAENIDKHDKMLEKINLTENIFIELINNISNLQKNFLYKETKKIIQKEFNQLRNIIHGITLLQQCPDNIHAIIVSRGEILSVLIMQSILQAKSYDITIIDPVKSFISCSHGYLDATINIDDSKKYISKLKIAQNTIILMSGFIAGNQNQELIVLGRNGSDYSAAILAVCLNAQCCEIWTDVDGVLTGDPNIISNPYLLQYISYQEAIELSYFGAKVLHPRTIIPISQFNIPCWIKNTNNIQSKGTLIF",
    "response#3": "MKVLKFGGTSLANAEKFLCVANIIEKNIITDQIAVVLSAPAKITNHLVNIAENIDKHDKMLEKINLTENIFIELINNISNLQKNFLYKETKKIIQKEFNQLRNIIHGITLLQQCPDNIHAIIVSRGEILSVLIMQSILQAKSYDITIIDPVKSFISCSHGYLDATINIDDSKKYISKLKIAQNTIILMSGFIAGNQNQELIVLGRNGSDYSAAILAVCLNAQCCEIWTDVDGVLTGDPNIISNPYLLQYISYQEAIELSYFGAKVLHPRTIIPISQFNIPCWIKNTNNIQSKGTLIP"
  },
  {
    "reference": "MPRIAVLIVAAGKGERAGTELPKQYERLAGKPMLRRTVEAFGGYPVQVVIGAGQQELAAQALAGLSVPSPVTGGVTRQESVRLGLEALAKDAPDFVLIHDAARPLVSRKVIEDVVKALEAGADGALPMVAASDTLRRAGDDGRWTLVNRENLYRAQTPQGFVYAKITKAHRAHAGTDVTDDVALAELAGLKVQMVEGEEKNIKVTRKQDFALAESLIGADVRTASGYDVHKFKDGDHIWLCGLKIPHTHGLEGHSDADVGLHAITDALLGCIGEGDIGQHFPPSDERWRGAASWKFLDHAASLVAAKGGTINHVDVTIICERPKVGPHRDAMKAKIAEILKIEESRVSVKATTTEGLGFTGRREGIAAQAMATVRL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have metal ion binding, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity to facilitate terpenoid biosynthetic process.2. The designed protein should be able to compete with endogenous proteins or inhibitors for a divalent metal cation binding.3. The presence of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domains is critical for the interaction of the designed protein with its intended targets.4. The protein must be engineered to have a high affinity for a divalent metal cation in order to increase its catalytic activity.5. For general function, the protein need meet that Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D- erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C- methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF). The designed protein sequence is ",
    "response#1": "MTRLGNGYDVHAFGPGDHVTLCGVQVPHEAGLLGHSDADVGMHALTDAIYGALAQGDIGRHFPPSDPQWKGADSAIFLAHAASMARDKGFRIGNADVTLICEAPKIGPHALAMAHRLSEIMGVEEARISVKATTSERLGFTGRREGIAAIATATLIVEGDEALHKLTYASDFASTAPPIRVGTGYDVHRLEAGEELWLCGVRIDHTHGLSGHSDADVAIHALVDAMLGAVGRGDIGQHFPPSDPQWKGAASDRFLAHAGKLVAEAGYALGNADVTIICEAPKIGPHRDGMRARLAEILGVDPSLISVKATTTEKLGFTGRSEGIACQAAVSLILRW",
    "response#2": "MVFRLGNGYDVHAFGPGDHVTLCGVQVPHEAGLLGHSDADVGMHALTDAIYGALAQGDIGRHFPPSDPQWKGADSAIFLAHAASMARDKGFRIGNADVTLICEAPKIGPHALAMAHRLSEIMGVEEARISVKATTSERLGFTGRREGIAAIATATLIVARESDVELPSLRTGVGIDVHGYDENQPMWLGGVYFPDEPGLAGHSDGDAVIHAMCDALLSAAGLGDIGSRFGTSAEEYAGAASEVFLRETLALVRGAGYRVGNVAVQVIAQRPRIAARRMELERHLGELVDAPVSVAATTSDGLGFTGRGDGLMAVATALVMR",
    "response#3": "SADNLKITRPEDFARAARILETRMDIRTGNGFDVHAFGPGDHVTLCGIDIPHTHGLVGHSDADVALHTVTDAIYGALARGDIGQHFPPSDPQWKGARSDIFLRHAAQLARDMGFTLTHVDCTLICETPKIGPHVQPMRESMADMLGIELDRVSVKATTSERLGFTGRGEGIACMATATLVQMVEGDEALHKLTYASDFASTAPPIRVGTGYDVHRLEAGEELWLCGVRIDHTHGLSGHSDADVAIHALVDAMLGAVGRGDIGQHFPPSDPQWKGAASDRFLAHAGKLVAEAGYALGNADVTIICEAPKIGPHRDGMRARLAEILGVDPSLISVKATTTEKLGFTGRSEGIACQAAVSLIVT"
  },
  {
    "reference": "MEPLKTHTGTIAVLDRVNVDTDQIIPKQFLKRVERTGFGQFLFFDWRFLPNGEDNPDFELNQPHAKQGTILVAGHNFGCGSSREHAPWAIKDYGFRVVIAPSFADIFYNNCFKNGILPVKLKQEEVTALMEKGKDTAYSLTVNLEAQTVTSEDGFEATFEIDGYWKEMLINGWDEIGLTLRYEEQIKQFEASRV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate.2. A protein that is capable of catalyzing reactions within the Amino-acid biosynthesis pathway with high efficiency and specificity.3. The protein should be optimized for stability and solubility when expressed in 3-isopropylmalate dehydratase complex where it will be involved in leucine biosynthetic process. The designed protein sequence is ",
    "response#1": "MRRTDVDTDQIIPAVYLKRVTKTGFEDALFAGWRKDPGFVLNQEPYRPGSVLVAGQDFGTGSSREHAVWALRDYGFRAVLAPRFGDIFRGNAGKQGLLAAVVAQSDIDLIWKALEAHPGTEVTVDL",
    "response#2": "MRRTDVDTDQIIPAVYLKRVTKTGFEDALFAGWRKDPGFVLNQEPYRPGSVLVAGQDFGTGSSREHAVWALRDYGFRAVLAPRFGDIFRGNAGKQGLLAAVVAQSDIDLIWKALEAHPGTEVTVDRL",
    "response#3": "MRRTDVDTDQIIPAVYLKRVTKTGFEDALFAGWRKDPGFVLNQEPYRPGSVLVAGQDFGTGSSREHAVWALRDYGFRAVLAPRFGDIFRGNAGKQGLLAAVVAQSDIDLIWKALEAHPGTEVTVDE"
  },
  {
    "reference": "MFSDIERAVKDIAAGRPVVVVDDEDRENEGDLIFAAEFATQELLAFMVRYTSGYVCVPLPEQEADRLDLPPQHHTNQDARGTAYTVTVDARTGVSTGISAADRAHTIRLLADAATQPSDLSRPGHVVPLRARPGGVLRRAGHTEAAIDLAVLAGARPAGVLCELVNDDGTMMRLPDLERFAETHDLALISIADLIAYRRRHEQQVERVVEVRLPTSHGEFRAVGYRALTDGAEHVALVHGDLGDGEDVLVRVHSECLTGDVLGSLRCDCGPQLDAALDRVAAEGRGVVLYMRGHEGRGIGLLHKLQAYQLQDRGLDTVDANLELGLPADARDYGTGAQILYELGVRSMRLLTNNPAKRAGLEGYGLQITGREVLPVRPHPENVHYLRTKRDRMGHLFDELGEVAEA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must bind to Zn(2+) in order to perform its enzymatic function.2. A protein that is capable of catalyzing reactions within the riboflavin biosynthesis, Cofactor biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway with high efficiency and specificity.3. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..4. The protein contains novel GTP cyclohydrolase II domains that confer a unique function or activity.5. The designed protein is the optimization of the Nucleophile; for GTP cyclohydrolase activity, Proton acceptor; for GTP cyclohydrolase activity to achieve high catalytic efficiency for GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.6. The designed protein should be optimized for 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, zinc ion binding, GTP binding, manganese ion binding, magnesium ion binding activity in order to improve riboflavin biosynthetic process. The designed protein sequence is ",
    "response#1": "ATATLPLKNFGDFTMTVFNSLLDGSEHFALMKPPAVINRVPLVRIHSECVTGDVLGSCKCDCGAQLEQSLAMLAVEGGVLIYLRQEGRGLGLANKLKAYALQEQGFDTVDANIKLGLPIDNRDYAVAYQILNHFGISILRLLTNNXXXVANLEKYGLKISERDPENFFPKREVDIKLPTDLGTFRAIAYTNQLDNKEHVALVKGKIEPDQPTLVRVHSECLTGDVFHSNRCDCGPQLDAALQQIEREGAGVLLYMRQEGRGIGLINKLKAYALQEQGFDTVEANIKLGFAPDLRDYGIGAQILKDLGVRQIRLLTNNPRKIRGLEGYGLEVVERVEAEAHLPTKFGTFKIYAYRNKLDGSEHVALVMGDINPDEPVLVRVHSECLTGDVFGSLRCDCQSQLYKALEIIAKEGKGVLVYMRGHEGRGIGIVNKIKAYALQEQGYDTVEANHKLGFNSDLRDFGTGAQILLDLGVRKMKLITNNPRKIVALEGFDLEIVDRVPPDEPARGFND",
    "response#2": "MSLERVSSVHLPTIYGDFMAHAYRFILDESPERLHIALVKGDLTAETPVLVRVHSECLTGEVFGSLKCDCGQQLHKAMELIEKEGMGVLLYMRQEGRGIGLLPKLKAYELQEKGLDTVEANLALGYPPDLRDYGVGAQILVHLGLRKIRLMTNNPKKIVGLRGYGLEVVERVPLRARPARGDEGTKIAEVELPTTRGFFRAFAYRDINDENLEHVHIALVKGDLRGRENILVRVHSECLTGDVFGSLRCDCGSQLAEAMNMIEKEGLGALLYMRQEGRGIGIAEKLKAYKLQEEGMDTVEANEALGFPADLRDYGIGAQILQDLGVKSIRLITNNPRKVVGLQGYGLEIVERVPLGLRAREADDRVSEAALPTDFGEFHIYAYETDVDSATHVALVKGDVGGRENVLVRVHSECLTGDVFGSLRCDCGSQLHRALEMVNAEGVGVVLYMRQEGRGIGLGNKLKAYHLQEQGLDTVEANIRLGFPADLRDYGIGAQILVDLGLHTIRLITNNPKKVIGLEGYGLEIVGRVPLVQRAAKVRLAETVLPTAIAGEFKAIVYESEVDPNHHLALVKGEIDSEQPILVRVHSQCLTGDVFGSLRCDCGSQLHTAMKMIEKEGKGVLVYMRQEGRGIGFANKMKAYALQDAGHDTVEANLALGFKPDLRNYGIGAQILRDLGVRKMRLMTNNPKKIVGLEGYGLEVVERIP",
    "response#3": "RLAETVLPTAIAGEFKAIVYESEVDPNHHLALVKGEIDSEQPILVRVHSQCLTGDVFGSLRCDCGSQLHTAMKMIEKEGKGVLVYMRQEGRGIGFANKMKAYALQDAGHDTVEANLALGFKPDLRNYGIGAQILRDLGVRKMRLMTNNPKKIVGLEGYGLEVVERIVAKAHLPTKYGDFMVHAYESSSDGGLPQRTHLALVKGDISGPEPVLVRVHSECLTGDALGSLRCDCGGQLGAALSQIEKEGRGCLIYMRQEGRGIGLANKIRAYALQDEGYDTVEANMKLGFPADLREYGTGAQILVDLGIHKLRLLTNNPKKIVGLSGYGLEVVERVPAREVPREVDIKLPTDLGTFRAIAYTNQLDNKEHVALVKGKIEPDQPTLVRVHSECLTGDVFHSNRCDCGPQLDAALQQIEREGAGVLLYMRQEGRGIGLINKLKAYALQEQGFDTVEANIKLGFAPDLRDYGIGAQILKDLGVRQIRLLTNNPRKIRGLEGYGLEVVERVERVSSVHLPTIYGDFMAHAYRFILDESPERLHIALVKGDLTAETPVLVRVHSECLTGEVFGSLKCDCGQQLHKAMELIEKEGMGVLLYMRQEGRGIGLLPKLKAYELQEKGLDTVEANLALGYPPDLRDYGVGAQILVHLGLRKIRLMTNNPKKIVGLRGYGLEVVERVPAKEVPAPA"
  },
  {
    "reference": "MADPGAAGAGVTRRRRATFAAGRGASLQPVNGGSVAARLSSLGQSGDSPGKKHGSPDATRLEGKSEKHKRRNDKPSDMLSCHRAQESLLSSASGYNNYRGVLNWCVVMLILSNARLFLENLLRHGILVDPIQVVSLFLKDPYSWPAACLVIVSNVFIIVALYTERQLSKGSFSEHVGFVVHCMNLTVLLTFPAAVILAVPSVTPVGGAFALGIYTILFLKLYSYRDVNMWCRDLSSAKAKKLSRSLSCPSGSSGDSSKVCYPGNLMIKDMYYYVFAPTLCYELNFPRSPKIRKSFLARRLVEMLFFTQLFVALTQQWMIPIIQSSMKPLEDMDLSRMMERLLRLAVPNHLMWLMFFYWFFHSSLNFSAELLRFGDRQFYKDWWNAETVTYFWQNWNIPVHKWCLRHFYKPLLQRGFSKKVSQSAVFFLSAFFHEYLVSVPLRMFRLWAFLGMMAQLPLAWFVGNFLHGNYGNAAVWMSLIIGQPFAVLMYVHDYYVLHYRLHTD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Target a Polar residues compositional bias in the protein's composition for improved properties.2. The protein should be optimized for activity in glycerolipid metabolism, Lipid metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. A key design requirement for the protein is the optimization of the active site to achieve high catalytic efficiency for hexadecane-1,2-diol + hexadecanoyl-CoA = 2-hydroxyhexadecyl   hexadecanoate + CoA.4. The designed protein should have retinol O-fatty-acyltransferase activity, diacylglycerol O-acyltransferase activity within endoplasmic reticulum membrane to regulate triglyceride biosynthetic process.5. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "MSLKCPRCGRPLTGKAGEPIRCPYCGTLITLEGQEYYLKVI",
    "response#2": "MLFWNLVVLFFAICPWLCLAQDPTSTIKN",
    "response#3": "MAARAPTLLLAALLLLQLSCNGSC"
  },
  {
    "reference": "MTNSTNDLVNFLGMDRAQLTTLFAELGEKPYRAEQMMKWMHHRQVSDFDDMTDMSKKLRSRLSELGRVHEAPVAESFESVDGTRKWLVRAASGSLVETVFIPENNRGTLCVSSQVGCALDCSFCSTGKQGFNSNLTSGEIVGQVRMANRLLADVYPDRAHAVTNVVMMGMGEPLLNFDNVVAAVDIMMDDLGYGISKRKVTISTAGVVPGIEKLIETTDCSLALSLHAPNDALRNELVPINRRYNIESLLDAARRYTRNLGEKRTVTVEYTVIDKVNDQPEHAVELAELLRSLPCKINLIPFNPFPGSGYRRPSSMNIRRFQDRLVRAGHSVTVRTTRGEDIEAACGQLKGKIADKTRRQEQYRMIASEAQA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein requires [4Fe-4S] cluster to activate a critical catalytic site.2. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity.3. A protein that contributes to rRNA base methylation by functioning in cytoplasm with tRNA binding, metal ion binding, rRNA (adenine-C2-)-methyltransferase activity, tRNA (adenine-C2-)-methyltransferase activity, rRNA binding.4. The protein should have a high affinity for [4Fe-4S] cluster, S-adenosyl-L-methionine.5. The protein's S-methylcysteine intermediate, Proton acceptor must be carefully engineered to ensure that the amino acids involved in adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "RTLCVSSQVGCAFGCKFCASGLDGFTRNLTADEIAGQVLLAEELSGEKVNNIVFMGMGEPLANLPQLLKALELITSPWGLGIGARHLTVSTSGLVPQIRKLADHPQQIRLAISLHGASNDVRDRIMPVNRKWPVEDLFKALSYWTKRKNQKITLEYILIDGINADPAQARLLAQHAESLRAKVNLIPYNTVDGLKWKRPPEEECHAFRDILAERVAMERQAELTAPGRKTVCVSSQVGCRFKCAFCASGQAGFRRNLEAGEIVAQTLWAALIWERRPSNVVFMGIGEPLDNYDNVLKAIRIINDSEGLGIGARKITISTSGIIPGMERLAGEGLQIELSVSLHAPTQELRSRLMPVARLYPLDRLIRACRSYFEKTKRLITFEYVLIRGTNDQASHARKLAELLSDFPCRVNLLLLSPVPEFKGTSSSPDAANAFIKTLSRAGINATLRAPRGLAADANRMAG",
    "response#2": "MEYAKANLLGMTKPQLEEFFESIGEKRFRAGQVMQWIHHFGAEDFDAMTNVGKALREKLKACAEIRGPEVVSQDISSDGTRKWVVRVASGSCVETVYIPQAGRGTLCVSSQAGCALDCSFCSTGKQGFNSDLTSAEIIGQVWIANKSFGTVPGKIDRAITNVVMMGMGEPLLNFDNVVTAMSIMMEDFGYGISKRKVTLSTSGVVPMIDKLGETTDVSLALSLHAPNDELRNQLVPINKKYPLKMLLDACRRYITRLGKERVLTIEYTLLSNVNDQPEHAEQMIALLKDFPCKINLIPFNPFPHSGYERPSNNSIRRFQDMLHKGGFNVTVRTTRGDDIDAACGQLVGQVMDRTRRSARYRQS",
    "response#3": "EDKRNTVCVSTQVGCALGCTFCRTGSMGFVRNLSSGEILSQLLLISRLSANPVTNVVFMGMGEPFLNFDALIEAAKMMNDPRAFNLSARRISISTAGIIPGIEQLSLLPWQFKLAISLNAIFPELRETLMPISKKYSLDNLLKAAKSYTEKSGKLVMFEYVLIDGVNDRDEDAAELKRRLKDIPCKINLIPFNSIATAMQRPSPQRIRRFISNLENAPFPVIVRHSGGTDMASNIAGLKKPPPHLADLDPAGRKALLEDNGLPGFRAKQLSTHYFARLVDAPEQMTDLPAAQRGELVSTLLPELMTPVRTSEADRGTTRKTLWRLFDGALVESVLMRYTDRTTMCVSSQAGCGMACPFCATGQGGLQRNMSTAEIVEQVMAGARSLARGEVPGGPGRVNNVVFMGMGEPLANYKAVVGAVRRLTDPAPDGLGMSARGVTVSTVGLVPRIRQLTEEGVPLTLALSLHAPDDELRNELVPINTRFSVAETVDAAWQYARTTKRRVSIEYAMMRGINDQGWRADLLADVLYATGADWGWVHVNLIPLNPTPGSKWTASDPADEQEFVRRLEARGIPTTVRDTRGREIDGACGQLAASS"
  },
  {
    "reference": "MRDKIKMVSSAGTGHFYTTDKNKRNTPDKLEMKKFDPVVRKHVMYKEAKIK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein can be targeted to ribonucleoprotein complex, cytoplasm, ribosome and perform structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "MPTVNLDRLWTLVPNETREKYLGKNTEVAPVINVLQSGYGKVLGKGRLPDTPVIIQTRYVSRRAEEKIKQAGGVVK",
    "response#2": "MPL",
    "response#3": "STVNLDRLWTLVPNETREKYLGKNTEVAPVINVLQSGYGKVLGKGRLPDTPVIIQTRYVSRRAEEKIKQAGGVVL"
  },
  {
    "reference": "MTRSFLSLSLIDTCWLSLALCFLVAFCVHKASAQLPGEYRKSSLFSLFQDLIRYGKTKQKLHRAQWLRVFDVPKRWFWHFYAISICWNSFLLLLSFNVIYQLQSYPSWLTRMLEVLTGEPSSFGQAPQLSTVLVQLLLCVHSLRRLLECQVISVFSDGAMHLVQYLFGLGYYIVLGLTVLCSDRTAKGPGTLLSRSDWIRAAGFVLFIAASLLQHQSIVLLAGLRKGKSGRVETLAHLVPEGGWFELVSCPHYFAELLIYVSLGFALGGFSFTWWLVVLYVLFNQALAAQLCHDLYVGKYESYPKHRRAFIPFVL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The ideal protein would be one that plays a critical role in polyprenol catabolic process, dolichol-linked oligosaccharide biosynthetic process through 3-oxo-5-alpha-steroid 4-dehydrogenase activity, cholestenone 5-alpha-reductase activity, polyprenol reductase activity within endoplasmic reticulum membrane.2. The protein should have Helical domains to ensure proper insertion into the membrane.3. The protein should be optimized for activity in protein glycosylation, Protein modification pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The protein contains novel Steroid 5-alpha reductase C-terminal domains that confer a unique function or activity.5. The protein must contain a signal peptide for proper functionality.6. For general function, the protein need meet that Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism.; Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N- glycosylation; Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol The designed protein sequence is ",
    "response#1": "MSDGVIHTVQYCFGLGYYVALGLTVLCQVPANFRNGKVINLSHSVPYGDWFERVSCPHYFAEVLIYVSMAITLGLHNVTWWFVVLYVLSNQALAAVLCHEFYQNKFSSYPKHRKAFIPFVSSPKEEEVVIEEDGVIHTVQYCFGLGYYVALGLTVLCQVPANFRNGKVINLSHSVPYGDWFERVSCPHYFAEVLIYVSMAITLGLHNVTWWFVVLYVLSNQALAAVLCHEFYQNKFSSYPKHRKAFIPFVSSPKEEEVAIEEEVVIEEEVAIEEKVVIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAMEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEEEVAIEE",
    "response#2": "MYKDGVIHTVQYCFGLGYYVALGLTVLCQVPANFRNGKVINLSHSVPYGDWFERVSCPHYFAEVLIYVSMAITLGLHNVTWWFVVLYVLSNQALAAVLCHEFYQNKFSSYPKHRKAFIPFVKKALLNPTHDYWYHIIGIVMYVWASLHQHRCLVILANLRKSKSGEVLSLNHSIPFGDWFERVSCPHYFAELLIYISMAITFGFHNVTWWFVVMYVFFNQTLAAVLCHEFYQEKFTSYPKHRKAFIPFIKK",
    "response#3": "MALVTMSFSGIPPLLSLSLSQLASLYGPVVLAVVLGFGIWIWQNICRYRAGRYPPGPWGFPLLGNLLQVDMKDIPKSFAKLSEKYGPVFTIHLGSRRMVVVSSPTLVKEVLLRQGEDFSGRGDFPTFELFSNGQNSIAFNSYGPVWREHRRFSVSTLRNFGMGKQGNEERIQQEFSYLIKEFQKSQGKPFDPTYLISTSVANVIADILFNRRFDYNDQTILRLMDLFNENFYLLSTPWLQLYNIFPGIMEYLPGPHQRILKSTEELKAFIAERVQKNLNTLDPNCPRDLVDAYLVEIEKEKANPNSTFTMENLVFSVGDLFGAGTETTSTTLRYGLLLLLKHPEVTAKVQEEIDCVIGKHRIPCMEDRSHMPYTDAVIHEIQRYADIIPSNLPHAVTCDVKFRNYLIPKGTDILTSLTSILHDESQFKTPHSFNPTHFLDEQGHFKKSDAFMPF"
  },
  {
    "reference": "MSQVAPKWYQSEDVPAPKQTRKTARPQKLRASLVPGTVLILLAGRFRGKRVVYLKNLEDNTLLVSGPFKVNGVPLRRVNARYVIATSTKVNVSGVDVSKFNVEYFAREKSSKSKKSEAEFFNESQPKKEIKAERVADQKSVDAALLSEIKKTPLLKQYLAASFSLKNGDRPHLLKF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should be able to enhance the efficiency of ribosomal large subunit assembly. The designed protein sequence is ",
    "response#1": "PIIAIVGRPNVGKSTLFNNLVGDKIAIVDDLPGVTRDRLYRDTEWSGSEFVIVDTGGLEPRNNDFLMAKIKEQAEVAMNEADVILFVVDGKSGLNPLDEEIAYILRKKNKPVILCVNKIDNFFEQQDDVYDFYGLGFEYLVPISGEHKVNLGDMLDIVVDIIGKMK",
    "response#2": "ADVGLVGLPNAGKSTFLKATTNAHPKIGNYPFTTLHPQLGVVRRHGQEFVLADIPGLIEGASEGIGIGDRFLGHIERCRILLHLIDASGEDPIAAWHEVQNELALYGAGLAEKPQLLALNKIDSVDEETCAELSQALEEASGQKVLLLSGATGQGLDPILDQL",
    "response#3": "ADVGLVGLPNAGKSTFLKATTNAHPKIGNYPFTTLHPQLGVVRRHGQEFVLADIPGLIEGASEGIGIGDRFLGHIERCRILLHLIDASGEDPIAAWHEVQNELALYGAGLAEKPQLLALNKIDSVDEETCAELSQALEEASGQKVLLLSGATGQGLDPILDQL"
  },
  {
    "reference": "MSEQQHTAKPDAEVGEMNEPEAAKCPVAHDRATRPGGTSNDEWWPNRLNLKVLAKNPAVANPLGDDFDYAAAFNSLDLAAVKSDIAAVLTDSQDWWPADFGHYGPFMIRMAWHSAGTYRVQDGRGGAGAGQQRFAPLNSWPDNGNLDKARRLLWPVKKKYGQKLSWADLMVLTGNVALETMGFKTFGFGGGREDVWEPDDDVYWGSEAEWLGDERYTGDRDLENPLAAVQMGLIYVNPEGPNGNPDPIAAARDIRETFARMAMNDEETVALIAGGHTFGKTHGAADPLVNVGPEPEAAPLEAQGLGWINTYGTGKGKDAITSGIEVTWTPTPTQWDNGFFDMLFGYEWELSASPAGANQWVAKDAEAIIPAPDEGGPKRLPTMLTTDLSLRFDPAYEQISRRFHENPEEFADAFARAWFKLTHRDMGPVERYLGPEVPQEELLWQDPLPAASGEPVTADDVTALKQAIADSGLTTSQLVKAAWASAATFRSSDKRGGANGARVRLAPQNGWDVNDPAELASVLRVLEGIQSDVNGRGGRTVSLADLIVLGGNVAVERAAAAGGITVEVPFRPGRVDASQEQTDVESFGYLEPKHDGFRNYLGKPGRVPAEYLLVDRANLLGLSAPQMTVLVGGLRVLGANTGGSSTGVLTDRPGTLTNDFFVNLLELGTTWTPTSEAADEFTATNAAGEQTWTGSRVDLLFGSNSELRALAEVYASDDAGEKFVRDFVAAWTQVMEADRFDVK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The heme b binding site should be stable and able to withstand changes in environmental conditions.2. We need a protein that can perform AH2 + H2O2 = A + 2 H2O with high efficiency.3. The protein design should emphasize Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "YPIFAQQNFKNPREANGRIVCANCHLAQKPVELEVPQAVLPDTVFEATVKVPYDKSIKQVGANGKPAELNVGAVLILPEGFQLAPSDRIPEEMKTKVGNLYFSPYSTEQKNILVVGPVPGKDYSEMVFPILSPDPAKDKSVSYLNYPVYLGGNRG",
    "response#2": "GDIEAGEKAFNKCKSCHQIVSDAGEEIVKGGRTGPNLYGVLGRQAGTADFRYGDDLVAAGEAGLVWDADNFVEYVTDPRAFLRAYLDDSKAKSKMAYKLRSGGEDIAAYLASVL",
    "response#3": "GDIEAGEKAFNKCKSCHQIVSDAGEEIVKGGRTGPNLYGVLGRQAGTADFRYGDDLVAAGEAGLVWDADNFVEYVTDPRAFLRAYLDDSKAKSKMAYKLRSGGEDIAAYLASVL"
  },
  {
    "reference": "MKSTFKEERIKDDSKRRDLFVFVRQTMCIAAMYPFGYYVNGSGVLAVLVRFCDLTYELFNYFVSVHIAGLYICTIYINYGQGDLDFFVNCLIQTIIYLWTIAMKLYFRRFRPGLLNTILSNINDEYETRSAVGFSFVTMAGSYRMSKLWIKTYVYCCYIGTIFWLALPIAYRDRSLPLACWYPFDYTQPGVYEVVFLLQAMGQIQVAASFASSSGLHMVLCVLISGQYDVLFCSLKNVLASSYVLMGANMTELNQLQAEQSAADVEPGQYAYSVEEETPLQELLKVGSSMDFSSAFRLSFVRCIQHHRYIVAALKKIESFYSPIWFVKIGEVTFLMCLVAFVSTKSTAANSFMRMVSLGQYLLLVLYELFIICYFADIVFQNSQRCGEALWRSPWQRHLKDVRSDYMFFMLNSRRQFQLTAGKISNLNVDRFRGTITTAFSFLTLLQKMDARE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have at least Helical; Name=4, Helical; Name=1, Helical; Name=3, Helical; Name=6, Helical; Name=7, Helical; Name=2 transmembrane domains to ensure proper localization.2. The ideal protein would be one that plays a critical role in olfactory behavior, detection of chemical stimulus involved in sensory perception of smell, signal transduction, protein localization through odorant binding within plasma membrane, dendrite membrane.3. The protein must exhibit the following characteristics: The odorant receptor repertoire encodes a large collection of odor stimuli that vary widely in identity, intensity, and duration.4. The Extracellular topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "GNTTIILVSRLEPKLHMPMYFFLSHLSFLYRCFTSSVIPQLLVNLWEPMKTIAYGGCLVHLYNSHALGSTECVLPAVMSCDRYVAVCRPLHYTVLMHIHLCMALASMAWLSGIATTLVQSTLTLQLPFCGHRQVDHFICEVPVLIKLACVGTTFNEAELFVASILFLIVPVSFILVSSGYIAHAVLRIKSATRRQKAFGTCFSHLTVVTIFYGTIIFMYLQPAKSRSRDQGKFVSLFYTVVTRMLNPLIS",
    "response#2": "GNTTIILVSRLEPKLHMPMYFFLSHLSFLYRCFTSSVIPQLLVNLWEPMKTIAYGGCLVHLYNSHALGSTECVLPAVMSCDRYVAVCRPLHYTVLMHIHLCMALASMAWLSGIATTLVQSTLTLQLPFCGHRQVDHFICEVPVLIKLACVGTTFNEAELFVASILFLIVPVSFILVSSGYIAHAVLRIKSATRRQKAFGTCFSHLTVVTIFYGTIIFMYLQPAKSRSRDQGKFVSLFYTVVTRMLNPLISAAYSL",
    "response#3": "GNVLIVGVVRADTRLQTPMYFFLGNLSCLEILLTSVIIPKMLSNFLSRQHTISFAACITQFYFYFFLGASEFLLLAVMSADRYLAICHPLRYPLLMSGAVCFRVALACWVGGLVPVLGPTVAVALLPFCKQGAVVQHFFCDSGPLLRLACTNTKKLEETDFVLASLVIVSSLLITAVSYGLIVLAVLSIPSASGRQKAFSTCTSHLIVVTLFYGSAIFLYVRPSQSGSVDTNWAVTVITTFVTPLLNPFIYTLRNKEVMNALKRLFC"
  },
  {
    "reference": "MRQRTIVCPLIQNDGAYLLCKMANDRGVFPGQWALSGGGVEPGERIEEALRREIREELGDKLQLSQITPWTFRDDVRLKTYPDGSTEQIYMIYLIFDCVSANREIEINEEFQAYAWVAPADLHRYDLNEATRLTLKMKGLL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must have high specificity towards dUTP diphosphatase activity, dTTP diphosphatase activity. The designed protein sequence is ",
    "response#1": "MRQRTIVCPLIQNDGHYLLCKMADDRGVFPGQWALSGGGVEPGERIEAALRREVHEELGEALQLTSITPWTFRDDIRVKTYADGRREEIYMIYLIFDCISANRDVTINEEFQEYAWVSPEDLSRYDLNVATRQTLTLKGLV",
    "response#2": "MRQRTIVCPLIQNDGHYLLCKMADDRGVFPGQWALSGGGVEPGERIEAALRREVHEELGEALQLTSITPWTFRDDIRVKTYADGRREEIYMIYLIFDCISANRDVTINEEFQEYAWVSPEDLSRYDLNVATR",
    "response#3": "LPKYFTDGSSGMDLRACIDKEINLNPNETVLISTGVSIDISDQKISGLILPRSGLSHKYGIILSNTIGLIDSDYQGPIMISMLNRSNKNFLISIGDRIAQIIFVPLIQIEWNIVNKFVKITNRKDMGFGHSG"
  },
  {
    "reference": "MFHTIEEALVELKQGRVIIVVDDEDRENEGDFVALADRIKPETVNFMITHGRGLVCVPLLEERAQALDLPPMVTHNTDRHGTAFTVSVDAHNTTTGISAFERADTIRQLIDPAAKPQHFRRPGHIFPLVARPGGVLRRAGHTEAAVDLAKLCGAYPAGVICEVINEDGTMARVPELSEIAQRFGLKMITIQELIKYRRRTETLIKREVSIHLPTEFGEFTVVGYTNDVDGKEHVAFVKGELDPEEPTLVRVHSECLTGDVFGSHRCDCGPQLHAALHRIAEEGKGVLLYMRQEGRGIGLINKLKAYKLQEEGLDTVEANVKLGFQPDLREYGIGAQILRDLGVRRMRLMTNNPRKITGLKGYGLEVSEVVPIVFPPRKENRNYMMTKKEKMGHLLPL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should interact with enzymes and substrates within the Cofactor biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4, riboflavin biosynthesis pathway to enhance or regulate its activity.2. The designed protein must be able to perform GTP binding under specific conditions. The designed protein sequence is ",
    "response#1": "LTTSMPTEKGNFSLHLYQDKIDNGYHLALVMGDKDFLSREPVLIRVHSQCLTGDIFHSLRCDCGAQLSRAMEMIAQEGKGVILYMNQEGRGIGLVEKLKAYALQDKGLDTVEANEALGYEPDLREYGIGAQILFDLGVRKIKLLTNNPRKIIGLEGYGLEVTERVQ",
    "response#2": "LTTSMPTEKGNFSLHLYQDKIDNGYHLALVMGDKDFLSREPVLIRVHSQCLTGDIFHSLRCDCGAQLSRAMEMIAQEGKGVILYMNQEGRGIGLVEKLKAYALQDKGLDTVEANEALGYEPDLREYGIGAQILFDLGVRKIKLLTNNPRKIIGLEGYGLEVTERVQ",
    "response#3": "LTTSMPTEKGNFSLHLYQDKIDNGYHLALVMGDKDFLSREPVLIRVHSQCLTGDIFHSLRCDCGAQLSRAMEMIAQEGKGVILYMNQEGRGIGLVEKLKAYALQDKGLDTVEANEALGYEPDLREYGIGAQILFDLGVRKIKLLTNNPRKIIGLEGYGLEVTERVR"
  },
  {
    "reference": "MKSSLVGASLVSTLGLVQAQTIQWNIEGRHPQPHLARRAKTTYEEAISNDRSEGGYFTEVTIGNPPQNITLQLDTGSSDVWVPWNCADICEEDKKGKSGCPLGSFDPDKSKTFKHVAPGMFGITFVDDSYVKGDYFEDHFELDGAVINNLTMGLAIKTNISYGLIGVGYAKNEASGSTTDVIYPNLPVAMYQAGYINTIAYSVWLNDLDAKAGSILFGGIDTAKYVGDMHRIDIQKLDGHYYHFVVALTSLVATSSSGSDVLTSDSFPIQAVLDSGTTLSYLPQDLAHEIWEEVGAVWSPYYGVAVLPCAYGRNHGNFTFEFAGPDGPSISVGMDELVLTMTSDAANMPAFESGVYKGENMCTFGIQNDTNDLYLLGNTFLRSAYVVYDLVNNEIGIAATDFNSTKSKRVPFKSYGATIPSATAASNQYRATEKPTVPGHNFTAAEGFQESDSNGNDDEDNGGSLLAPGTTPLALVVTIMSFSNGASIIGNPLLNFFAEVQDCKMRATWAARWLNCRLLLSTQQPSCGNHSFAISAAAPAWETRRAFSQTKRSCSAAQEALKKAQEDAASLTPEYVAANMSTEEAKRLSRVRNIGIAAHIDSGKTTVSERVLFYTGRINSIHEVRGKDSVGAKMDSMELEREKGITIQSAATFADWKKMENGKEETYHFNLIDTPGHIDFTIEVERALRVLDGAVMILCAVSGVQSQTITVDRQMKRYNVPRISFVNKMDRMGANPWKAVEQINTKLKIPAAAIQIPIGAEDEFLGVV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. We require a protein that can specifically bind to the metabolites produced in the Protein biosynthesis, polypeptide chain elongation pathway.2. The protein contains novel Tr-type G domains that confer a unique function or activity.3. Incorporate a signal peptide in the protein design.4. The ideal protein would be one that plays a critical role in proteolysis through GTPase activity, translation elongation factor activity, aspartic-type endopeptidase activity within mitochondrion.5. The protein must exhibit the following characteristics: Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.; During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively.6. Include a signal peptide as an essential component of the protein.7. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.8. The designed protein should have a high specificity for GTP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "RYRNIGIMAHIDAGKTTTTERILFYTGVSHKIGEVHDGGATMDWMEQEQERGITITSAATTTHWSGMAQQFPEHRINIIDTPGHVDFTIEVERSLRVLDGAVTCFCSVSGVEPQSETVWRQADKYGVPRMGFVNKMDRAGANFLNVNQMVIDRLGALPVPMQLPIGAEETFRGVVDLVKMKAIYWEEENMGMKYTYEDIPEDMVELCEEWREKMVESAAEANEELMDKYLEEGDLSEEEIKAGIRQRCIDVEIVPMFCGSAFKNKGVQALLDAVIEYMPAPTDVPPIQGVAPDETLAALAFKIMSDQHLGKLTYLRIYSGVLESGMQVLNSIKGRKERIGKIYRMHANKREEITRVGAGDIVAVVGLKDTTTGETLCDPNHPIILERMEFPEPVISIAIEPKTKADQEKMGIALQKLAAEDPSFRVKTDEETNQTIISGMGELHLDIIVDRMKREFNVEANIGKPQVAYRETITKKVKVEGKFVRQSGGRGQYGHVWLEVEPQPEPGKGYEFVDAIKGGVVPREYIPAVDKGIQEATDNGVLAGFPVVDVKVTLIDGSYHEVDSSEMAFKIAGSMGFKEGCQKAHPVLLEPMMAVEVETPEDFMGNVMGDLSSRRGIVQGMEDIAGGGGKLVRAEVPLAEMFGYSTSLRSATQGRATYTMEFKHYAETPSNVSEAIIK",
    "response#2": "KNTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETHEGASQMDWMEQEQERGITITSAATTAQWNGHRVNIIDTPGHVDFTVEVERSLRVLDGAVAVLDAQSGVEPQTETVWRQATTYGVPRVVFVNKMDKIGADFLYSVKTLHDRLQANAYAIQLPIGAEDDFKGIIDLVEMKTYMYNNDLGTDIEVTDGFPADMADQAEELRGQLIEGVADFNEELMMKYLEGEEISIDELKAAIRQATLSVEFYPVLVGSAFKNKGVQLMLNAVVDYLPSPTDVPPVRGVDVILEPMMRVEVVIPEEYMGDIMGDVTSRRGRVEGMEARGNAQVVKAMIPLSEMFGYATSLRSRTQGRGTYSMHFDHYEEVPKSIAEEYKEALTNSIEHREIYKKQTGGRGKYADILFKLEPGKKGAQGLEFINKVKGGNIPKEYIPSIEKAFKEAMKNGPLYGYEINNARIILIDGSYHSVDSDQLSFELASKIGFKNAAKKTGTVILEPMMAVEVITPEDYMGDVIGDLNSRRGQIQAMEERSGARVVKALVPLSEMFGYIGDLRSKTQGRANYSMVFDSYAEVPANVSKEILEPIMKLEVLTPPENMGNIVGDINRRRGIIQGMEENRSNSKIIKALVPLSELFGYVTILRTLSSGRATSTMEFYKYKPKP",
    "response#3": "NTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETHEGASQMDWMEQEQERGITITSAATTAQWNGHRVNIIDTPGHVDFTVEVERSLRVLDGAVAVLDAQSGVEPQTETVWRQATTYGVPRVVFVNKMDKIGADFLYSVKTLHDRLQANAYAIQLPIGAEDDFKGIIDLVPQREGDAGKPFSALAFKIVSDPFVGRLVYFRIYSGSISAGSGAYNSTRGERERIGRLIRMHANDREEIEYADAGEIVASLGLRNTFTGDTLCSALSAPIIKFPDPVIGLAIEPKYKSDIDKMSLALSKLSEEDPTFIVRNDKYTGQTIISGMGELHLEIILDRMEREFKVKVNQYRETITKTVEKLEYTHKKQTGGSGQFAKVIIALEPFVGEDGAHYEFENKVTGGRVPKEYIPSVDAGAQDAMQYGVLAGYPLVNLKVTLLDGAYHDVDSSEMAFKIAGAQALKEAARKANPILLEPIMSVEVVVPEEYMGEVIGDLNSRRGRIMGMDSRAGAQVVSSMVPLANMFGYSTDLRSATQGRATYAMTFDHYEPVPKSVSDEIVYKEALTNSIEHREIYKKQTGGRGKYADILFKLEPGKKGAQGLEFINKVKGGNIPKEYIPSIEKAFKEAMKNGPLYGYEINNARIILIDGSYHSVDSDQLSFELASKIGFKNAAKKTRK"
  },
  {
    "reference": "MSNDQGSQFLRAPIVVVLGHVDAGKTTLLDKIRGTAVAKREPGTMTQHIGASFLPWKALEAVCGSLVSQIRAEVVIPGFLVIDTPGHEAFSNLRRRGGSIADIAILVVDVLRGLEQQTFESIDILRERKVPFIVAVNKIDKIPGWKSFPNTPFVESVKRQSEAAQLKLEELLSYIIQQFASLGFRSDRYDRIRDFTRVLALVPVSAVTGEGIPDLLLVLAGLAQRYLKGRLLASIAPGKGVILELKEEAGLGMTATLILYDGVIRRGDIVVTGGIEGAFSTRVRALLMPKPLDEMRSPEDRFLEVERIVAAAGVKLVAEGLEKAVPGAPLFVAVSEEEVGRLKQLVEEEISGVKFERDVVGVVVKADTLGTLEALVGYLKKQGIPIRVADIGPVVKRDVVQASMVKEKDPLYAAILAFNVKILPEAQDEAARHGIPVFQERIMYKLVENYQKWLQETRDAEVRKAFEKITPPAVVQILPGYVFRRRDPIIVGVRVVCGRIRSGVPLITKDGREIGEIMQIKEHDKVLDVVSEGAEVAISIRSKAIVGRQVKEGDYLYSNLSIEEINRLLEKYEKYLAENEKSYLRKLMRFKMGLSKEIEYP",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein contains novel tr-type G domains that confer a unique function or activity.2. The designed protein must be able to perform GTP binding, translation initiation factor activity, GTPase activity under specific conditions.3. For general function, the protein need meet that Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes The designed protein sequence is ",
    "response#1": "IVTVMGHVDHGKTSVLDVLRSANVVSGEFGGITQHIGAYQIQHESNKLTFIDTPGHAAFTEMRARGSKLTDVVVLVVAADDGVKPQTIESIKHAKAANVPIVVAINKCDLPEADPQKIKNQLLEYELIAEDLSGDTLMVEISAKNKKNLDKLVESL",
    "response#2": "IVTVMGHVDHGKTSVLDVLRSANVVSGEFGGITQHIGAYQIQHESNKLTFIDTPGHAAFTEMRARGSKLTDVVVLVVAADDGVKPQTIESIKHAKAANVPIVVAINKCDLPEADPQKIKNQLLEYELIAEDLSGDTLMVEISAKNKKNLDKLVESGRSLAACEGALLS",
    "response#3": "KNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPSIVVFLNKADQVDDEELLELVELEVQETLSEYEYPGEEIPIISGSALLALEALTENPELDSANNEWVQKIYHLMDSVDDYIPAE"
  },
  {
    "reference": "MATELQCPDSMPCHNQQVNSASTPSPEQLRPGDLILDHAGGNRASRAKVILLTGYAHSSLPAELDSGACGGSSLNSEGNSGSGDSSSYDAPAGNSFLEDCELSRQIGAQLKLLPMNDQIRELQTIIRDKTASRGDFMFSADRLIRLVVEEGLNQLPYKECMVTTPTGYKYEGVKFEKGNCGVSIMRSGEAMEQGLRDCCRSIRIGKILIQSDEETQRAKVYYAKFPPDIYRRKVLLMYPILSTGNTVIEAVKVLIEHGVQPSVIILLSLFSTPHGAKSIIQEFPEITILTTEVHPVAPTHFGQKYFGTD",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have GTP binding and be active in cytoplasm, nucleoplasm, cytosol, intracellular membrane-bounded organelle.2. The protein should exhibit Polar residues compositional bias.3. The 5-phospho-alpha-D-ribose 1-diphosphate, uracil, GTP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "VPPHPLIKHWLAVARDAATPSVLFRSAITELGRWLTYEAAREWLPTEETTVQTPLEICPATVINPQVPVAVVPILRAGLGLLEGAQTVLPLASIYHLGLVRNEETLEASCYLNKLPEKFDPQTRVLITDPMLATGGSIMKAMSELTERGVDPSLVRIVSVVAAPPALQKLNAAYSGLIVYTATIDETVNDQGFIVPGLGDAGDRIFGR",
    "response#2": "DSPYLMETLTDLRKISTENISFRKGLVRLGRYMGYELTKTMEFEKMPIQTPLEKTEGVFAKDRKNVVIITILRAAFPLMEGLIKNFESAKVGIVSASRGHAPDFKIEMNYIKVPQLNPEDTVIVSDPMIATGSTLIHVLKEFRDSKPKRMMIVGVLAAPEGINAVKKEFSDVEIFVTKIDENLNKDGYIVPGLGDAGDRAFIYHGNSNPELARKIARWLGTDCGKAEVFQFANENIFVKILDNVREKDIFLVQPTSHPVNQSIMELLIMIDAFKRASAGRITAVIPFYAYGRSDKKDQPRVPITARLIADMITVAAQ",
    "response#3": "DSPYLMETLTDLRKISTENISFRKGLVRLGRYMGYELTKTMEFEKMPIQTPLEKTEGVFAKDRKNVVIITILRAAFPLMEGLIKNFESAKVGIVSASRGHAPDFKIEMNYIKVPQLNPEDTVIVSDPMIATGSTLIHVLKEFRDSKPKRMMIVGVLAAPEGINAVKKEFSDVEIFVTKIDENLNKDGYIVPGLGDAGDRAFL"
  },
  {
    "reference": "MNQLIQAQKKLLPDLLLVMQKRFEILQYIRLTEPIGRRSLSASLGISERVLRGEVQFLKEQNLVDIKTNGMTLTEEGYELLSVLEDTMKDVLGLTLLEKTLKERLNLKDAIIVSGDSDQSPWVKKEMGRAAVACMKKRFSGKNIVAVTGGTTIEAVAEMMTPDSKNRELLFVPARGGLGEDVKNQANTICAHMAEKASGTYRLLFVPGQLSQGAYSSIIEEPSVKEVLNTIKSASMLVHGIGEAKTMAQRRNTPLEDLKKIDDNDAVTEAFGYYFNADGEVVHKVHSVGMQLDDIDAIPDIIAVAGGSSKAEAIEAYFKKPRNTVLVTDEGAAKKLLRDE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for beta-D-fructose 1,6-bisphosphate binding.2. The protein must be able to DNA binding, carbohydrate binding.3. For general function, the protein need meet that Binds specifically to the cggR-gapA promoter region and blocks the progression of the RNA polymerase, leading to the arrest of the transcription.; In the absence of glucose, represses the transcription of the gapA operon, which encodes five key glycolytic enzymes4. The protein's architecture should predominantly feature helix-strand-turn-helix-strand-helix-strand-strand-helix-helix-helix-helix-strand-helix-helix. The designed protein sequence is ",
    "response#1": "LEETLAPFRLNHDQLAAVQAQMRKAMAKGLRGEASSLRMLPTFVRATPDGSERGDFLALDLGGTNFRVLLVRVTTGVQITSEIYSIPETVAQGSGQQLFDHIVDCIVDFQQKQGLSGQSLPLGFTFSFPCRQLGLDQGILLNWTKGFKASDCEGQDVVSLLREAITRRQAVELNVVAIVNDTVGTMMSCGRATVLGHIQRGGSPVPYDLAPCVKVLNEAFGVEKGYMVTVHAYTNDQRLLDLPHKDFRRARAAAINIVPTTTGAAKAIGEVIPELKGKLDGTARRVPVPDGSLIDLTVVVNKAPSSVEEVNEKFREAAQKYRESGKVYLKEILQYCEDPIVSTDIVGNPHSAIFDAPLTQVIDNLVHIAAWLGLS",
    "response#2": "LAPCVKVLNEAFGVEKGYMVTVHAYTNDQRLLDLPHKDFRRARAAAINIVPTTTGAAKAIGEVIPELKGKLDGTARRVPVPDGSLIDLTVVVNKAPSSVEEVNEKFREAAQKYRESGKVYLKEILQYCEDPIVSTDIVGNPHSAIFDAPLTQVIDNLVHIAAWEHVERAIDLLNGARRIEFYGLGNSNIVAQDAHYKFFRFGIPTIAYGDLYMQAASAALLGKGDVIVAVSKSGRAPELLRVLDVAMQAGAKVIAITSSNTPLAKRATVALETDHIEIRESQLSMISRILHLVMIDILAVEK",
    "response#3": "VGISLEKKGRVHLVGWQTLGIIAIMDGVECIHTFGADFQDIRGFLIGDHSDMFNQKDELTNQGPQFTFSQDDFLTSILPSLTETDTVVFIFTLDDNLTEVQALAERVREKCQNIQALVHSTVGQSLPAPLKKLFPSLISITWPLLFFDYEGTYVQKFQRELSTKWVLNTVSTGAHVLLGEGQ"
  },
  {
    "reference": "MGIELLKQEHYRIFDQTSLGNTFHATQSFAMDDTLCASVATEGAAIRSWVHHETVVLGIQDARLPHLDDGIDVLHAHGFQPVIRNSGGLAVVLDAGVLNISLVLPERGGIDIDSGYEAMLALVRRMFAEETDAINAGEVVGSYCPGSYDLSIAGKKFAGISQRRVRGGVAVQIYLCVNGSGSARAQLVRDFYAAALQGETTKFVYPTVVPETMASLEELLQRPLTVEDCLVRVYRSLMELGATLTPSVLSEVENERFGVNLGRMLDRNEKVLG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Catalyzes the amidotransfer (transamidation) of the octanoyl moiety from octanoyl-GcvH to the lipoyl domain of the E2 subunit of lipoate-dependent enzymes.2. The designed protein should contain one or more BPL/LPL catalytic domains that are essential for its function.3. A protein with octanoyltransferase activity is needed to enhance protein lipoylation, lipoate biosynthetic process in a specific context.4. The protein should be optimized for activity in Protein modification, protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein], protein lipoylation via endogenous pathway pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. The protein with the reaction L-lysyl-[lipoyl-carrier protein] + N(6)-octanoyl-L-lysyl-   [glycine-cleavage complex H protein] = L-lysyl-[glycine-cleavage   complex H protein] + N(6)-octanoyl-L-lysyl-[lipoyl-carrier protein], the Acyl-thioester intermediate must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MLKKEDNQLFFCEHLPVLTIGKSGKDSNLLIPEETLRERGVSFYHINRGGDITYHGPGQITGYPVFDLEYWNLGLKQYIHMLEETIIRFLSLYDLKGERLEGATGVWLDPEVPGRARKICAIGVKSSRFVTMHGFALNINTDLSYFSLINPCGFTDKGVTSLAMELGVPQDFELAKSQLRSIFMEIFQKYLGKDGEVAPTLRFYSWEPRGLSVGHFQRATKDIDRNRIEALGIPIVRRMTGGRAVLHADELTYSVILPEQMEGVPKTVIESYRMLTEGIRKGYHHLGIPVEFSVPMTEEEKEELRKPKSAVCFDAASYYELAVGKRKVAGSAQVRHQGVVLQHGSVPLSVDEGELFDCFLYEDETMRERMKARFAGKAVALNELAGRSVSFEEVRLAFVKGFEDALQLTPDTPDQLWLVEHPPVFTQGQAGKAEHLLFPGDIPVVQTDRGGQVTYHGPGQLVAYPLLDLRRLKMGVRDLVTAIEQTIVATLAEYGIESYPKPDAPGVYVENHKIASLGLRVRRGCSFHGLALNVDMDLSPFLRINPCGYQGLAMTQMRDLMPETPSLVQVQEQLVCQFARKLGYEDFGIEHIYAQGLELQRQLVEARHSGRIGNTLLLLEHPPVLTLGRNSERKNVLASDEFLAYRGVEIHEVNRGGDVTYHGPGQLVGYPILDLRSFAESGERGRLGAVEYVRWVEEALIRTCADFGVQTQRVAGRTGVWTLPGGSVEEKKIAAIGVHISRGITSHGFALNVTTDLRDFDLIVPCGISDRKVTSLELEVIDEPALTMEKVIHSAARQ",
    "response#2": "MRAFTAGRGAATPDEVWLCEHAPVYTLGQAGKPEHLLNPGAIPVVMCDRGGQVTYHGPGQVVAYTLFDLRRAGLFVREYVRLLEDAAIGTLDDFGVPGACRKPGAPGVYVPLGPAGELAKIAALGIKIRNGYAYHGVALNVDMDLSPFLGINPCGYEGLVVPLGGEADLADAALAIQEQLAAGVAAGTSPETLLLLEHPPVYTIGRGGCRENVLDPGIRVVEANRGGDVTFHGPGQLVGYPVIDLGRRGRDLHRYLRILEELLINVAAEFGVAARREPGRTGIWTDGGKLASIGVGVRRWVTMHGFALNVTNELAPFRSINPCGIVACPITSLSAILGRPVPLGDVRRSVAGRGRIGNTLLLLEHPPVLTLGRNSERKNVLASDEFLAYRGVEIHEVNRGGDVTYHGPGQLVGYPILDLRSFAESGERGRLGAVEYVRWVEEALIRTCADFGVQTQRVAGRTGVWTLPGGSVEEKKIAAIGVHISRGITSHGFALNVTTDLRDFDLIVPCGISDRKVTSLELEVIDEPALTMEKVIHSAARQFGRVFGHQLVRRGEAAGDYRAAWQQQRDLADARVAGGPDTLLLLQHPAVYTAGRRTEPHERPLDGTPVVDTDRGGKITWHGPGQLVGYPIIGLAEPLDVVDYVRRLEEALIKVCADLGLDTVRVPGRSGVWVPGDAGRPDRKVAAIGVRVSRATTLHGFALNCDCELGAFNAIVPCGISDAGVTSLTAELRRPVAVDDVVTSVADAATPDEVWLCEHAPVYTLGQAGKPEHLLNPGAIPVVMCDRGGQVTYHGPGQVVAYTLFDLRRAGLFVREYVRLLEDAAIGTLDDFGVPGACRKPGAPGVYVPLGPAGELAKIAALGIKIRNGYAYHGVALNVDMDLSPFLGINPCGYEGLVTVDMAACGVRRDLVDVGQRLAERLLAAMPEP",
    "response#3": "MRAFTAGRGAATPDEVWLCEHAPVYTLGQAGKPEHLLNPGAIPVVMCDRGGQVTYHGPGQVVAYTLFDLRRAGLFVREYVRLLEDAAIGTLDDFGVPGACRKPGAPGVYVPLGPAGELAKIAALGIKIRNGYAYHGVALNVDMDLSPFLGINPCGYEGLVGKTPPTLRFYAWSPPTISLGYFQQLEKEIDLEAVKERGLGLVRRLTGGRAVLHDDEVTYSVVAREDHPLMIGGIRPSYLRLAKALAAGLRELGAPVEIASGRKGGREEHTTAACFDAPSWYEITCGGRKLVGSAQTRKGGVVLQHGSIVLTLNGDDLFAVLKMPSEAVRQRLLAKFYHQACGLEEVLGRRVEAGVIKENIVRAFTRLYAVEKLKQLRLLNFEVLLDAKATGKKEDNQLFFCEHLPVLTIGKSGKDSNLLIPEETLRERGVSFYHINRGGDITYHGPGQITGYPVFDLEYWNLGLKQYIHMLEETIIRFLSLYDLKGERLEGATGVWLDPEVPGRARKICAIGVKSSRFVTMHGFALNINTDLSYFSLINPCGFTDKGVTSLAMELGVPQDFELAKSQLQELMPLLDKRYQPTLESMRQLTRERDERTADEIWLLQHPKVFTQGQAGKAEHLLAPGDIPVVQVERGGQVTYHGPGQLVAYLMLDLRRLDLGVRELVTAMEQSLVDLLASYGIEAAPKADAPGVYVAGDKIASLGLRVSRGCSFHGLALNVDMDMTPFLRINPCGYAGLKMVQMRDLLPSPPAFDEVSQRLEQAYELSRAGLADWLLYQPTLESMRQLTRERDERTADEIWLLQHPKVFTQGQAGKAEHLLAPGDIPVVQVERGGQVTYHGPGQLVAYLMLDLRRLDLGVRELVTAMEQSLVDLLASYGIEAAPKADAPGVYVAGDKIASLGLRVSRGCSFHGLALNVDMDMTPFLRINPCGYAGLKMVQMRDLLPSPPAFDEVSQRLEQA"
  },
  {
    "reference": "MFLEERRSQILQWLDEHERVTVNGLASELGVTRETIRTDLSALAQQGLVQRCYGGAIALRRNLQEKLISESGNFEVLLQRLQSRKASGKSSGKQGKVCILGAFNVDIVAKVACFPQSGESLMALGSTLGPGGKGANQAMAASCAGTRVHFVAKVGTDQFSQFAFDHLSASDIHSFRLYQTDSEPTGSAIIYVSQENGENMIAIYSGANKTVTDEEIAELTHELTDADVMLLQLENNFEATRSAIRLAHTLGVRVIMNPAPFSADVVSCLPMIDVLTPNETEASRLSGIEIFDLTSARAAAQAIVALGARRLIITMGSRGALLFDNQKFHHIPAFPALSVDTTGAGDAFNGALAAALAQGQSLLQAATWASAFASLAVEREGASNMPDRQQVLARLQQR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein contains novel HTH deoR-type domains that confer a unique function or activity.2. The protein should be able to bind substrate ligand in a variety of conditions3. The designed protein should be optimized for ATP binding, ribokinase activity, metal ion binding, DNA binding activity in order to improve D-ribose catabolic process.4. The H-T-H motif domain within the designed protein should be capable of recognizing and binding to a range of DNA sequences. The designed protein sequence is ",
    "response#1": "YEEKQLIARKALPFIDNKTTMAADSSSTVMELLKLLQDRSGLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEIIRRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFVQLADFSHINYIITDKVCILGSFNVDIVARVERFPKSGETLIASENTLGPGGKGCNQAMAAFYADAKVHFVAKVGTDQFSQFARNHFKSSGMDSYTLYQTAEYPTGSAVIYVSEQDGENMIAIAPGANQTITPEEINELLPELNSATVLLVQLENNIDAVGNIIDLAHTLNKMVIVNPAPYSPEIKPYLHKVDILTPNETEASLLANIEVTDIDSATRAAKIILSYGVKNVLITLGSQGVLLAENGHVTHLPAFPAVVVDTTGAGDSFNGALAASLANGKDLLQAAHYAAAFASLAVEREGAANC",
    "response#2": "KVCVFGSFNVDIVARVERFPRGGESLLALASSLGPGGKGANQATAASKAGAQVHFVAKVGKDQFSHLAADHLTRSEIHSYTLYQSESEPTGNAIIYVSQENGENMIAIYSGANTTITHNEIEAIIPELSASDVLLVQLENNFDATHSLIKIARELGKTVILNPAPYSAEIIKSIPYIDVITPNETEASLLSGIDIHDVDTAKEAALRIAELGAKKVLITMGSRGALLLDNRQFSQIRVFPAVTVDTTGAGDAFNGALAASLAAGNTLVQAATWASAFASLAVELEGASRQ",
    "response#3": "RVCILGSFNVDIVAKVARFPRGGESLLALGSTLGPGGKGANQAMAASRTGARVHFVSKVGKDQFSQFAFDHLSASDIHSFKLYQSESEPTGNAIIYVSQQDGENMIAIYSGANQTLTDAEVAEMTHELSGADVLLLQLENNFSATLNAMKLANTLGVRVIMNPAPFSDDVLKCLQYVDVITPNETEASLLSGVEVTDLESAREAAQAIVRQGAKRVIITMSSRGALILDNGQFQHIPAFPALSVDTTGAGDAFNGAFAAALASGQTIAQAATYAAAFASLAVEREGASNC"
  },
  {
    "reference": "MKVAVVGATGLVGQVMIKLLEERNFPLTELIPVASEKSVGKKISFKGQEVSVQSIQSAIDLKPEIALFSAGGETSLAYAEKFAAVGTTVIDNSSAWRMDPTKKLIVPEVNAGELTKEDKIIANPNCSTIQMVLTLAPIHKKYGIDRIVISTYQSITGTGVKALEQLKNEQSGKSGEMAYAYQIHENCIPQCDVFMENDYTKEEMKLVNEPKKIMGDDSLKITATAVRVPVMGGHAESINLSLKNSFEVAEIKELLSKTKGIVVKDNPANAEYPMPLYSKDKDEVFVGRIRRDESQENGLNLWIVADNLRKGAATNTIQIAEYLLEKGLV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The NADP(+), phosphate binding site should be stable and able to withstand changes in environmental conditions.2. A protein with protein dimerization activity, NAD binding functions is required to enable diaminopimelate biosynthetic process, 'de novo' L-methionine biosynthetic process.3. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate. The designed protein sequence is ",
    "response#1": "LGALHREFELKELVVASYQAASGAGQDGIDRLYAEYAAVAGKEVGNRAGDVREALAAAGLSQDDSPFPAPLALNVVPWAGSLKADGWASEELKVRNEARKILGIPDLKVSATCVRVPVVTTHSLAVHATFARPVTVEQAHAIFAAQPSIVLVDDPANGQFPTPADVVGGDPTYVGRVRQALDFPNTLDFFVCGDNLRN",
    "response#2": "LKPLYDHYGIEKVIVSTYQAVSGAGWQAINELEEQSKAVLEGKEVSAHVLPVGRLNKHYQQAFNVIPQVDVAEENGFTLEEMKMIRETKKIFGDDSIDVTATCVRVPVMRGHSESVYVELKSDFDLEEVRKQLGEVPGVVVQDAIGEQVYPMPINAEDRTEVFVGRLRRDLNNPRALNMWVVSDNLLR",
    "response#3": "LKPLYDHYGIEKVIVSTYQAVSGAGWQAINELEEQSKAVLEGKEVSAHVLPVGRLNKHYQQAFNVIPQVDVAEENGFTLEEMKMIRETKKIFGDDSIDVTATCVRVPVMRGHSESVYVELKSDFDLEEVRKQLGEVPGVVVQDAIGEQVYPMPINAEDRTEVFVGRLRRDLNNPRALNMWVVSDNLLN"
  },
  {
    "reference": "SDNNVEGEFVDPKPVLRIKMPHITSTVFKLYSDGAKDMEDWCTVEAFRPHTRTSCGFNSSHPLIIITHGWSINALMESWVPVLASALKTSLGDVNVIVTDWLLQANINYPNAAQNTMAVGKDIAFLLQWLQEFYHLGAHISGFAGSYLGGPGKIGRITGLDPAGPLFEGMAASDRLSPDDALFVDAIHTYTQERMGLSVGIKQPVAHVDFYHNGGDFQPGCHPFQSLMEHIALHGINGFEQTVKCAHERSVYLFIDSLLNKDKQIMACRCSDDNAFEKGTCLDYRKNKCNTLSYNVRRVRSVADKKLYNKTHSSMYVGNTVECQKSFLNKQENVIEEISGNKTYTFLITLDSDIGDLMMLMFRWEGSTFNPWGSGGMRPKLTVGKIRVKAGETQNRWVSIPLGGNEGVRLNCINKDQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein have Lipase, PLAT domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MLKCTIKNEQIETLRSGDTFVSYEIETESDLPVFEDKKFSVRRRYKDFEMLHNILSHDYNGYAIPPLPRKYTVSSFSGGSLSPIFIARRMQSLQTFLDRCSTHPVISNSMHMYQFLENNSWKR",
    "response#2": "SCGFNSSNPLIIITHGWSVDGMMESWVFRMATLLKANAVNVNVVITDWMSLACQHYPLAVKSTRTVGKDIAHLLQKLQEKYQYSARKAHLIGYSLGAHISGFAGSFLSGSEKIGRITGLDPAGPLFEGMSPTDRLSPDDAEFVDAIHTFTHERLGLSVGIKQAVAHYDFYPNGGDFQPGCNLQNIYEHISEYGISGFEQTVKCSHERSVHLFMDSLLNKDKQSTAYLCSNNNAFGRGVCLDCRRNRCNTLGYNIKKVRTAKSKKLYLNTRL",
    "response#3": "MLKCTIKNEQIETLRSGDTFVSYEIETESDLPVFEDKKFSVRRRYKDFEMLHNILSHDYNGYAIPPLPRKYTVSSFSGGSLSPIFIARRMQSLQTFLDRCSTHPVISNSMHMYQFLENNSWKC"
  },
  {
    "reference": "MNLSQKKILLGLTGGIAAYKVADLCRQLVKAGAEVKVVMTKGAKAFITPLTMQALSGNPVHDDLLDPSAETAMGHIELAKWPDLIVIAPASADFIARATAGMADDLLATLCVATAKPIALVPAMNQQMWANQATQANIKTAAKRSFHIWGPASGDQACGDVGFGRMIEPLEIAQKVDAFFNHKPLKSQHWVITAGPTVEKIDPVRYLTNRSSGKMGYAIAQAAANLGAKVTLVSGPVSLDSPNGVERVMVQSAQDMLQAVESATENSDVFVACAAVSDYRAKNVAEQKMKKQGDDGLSIELEQNPDILQWVASNRDCFAVGFAAETQNLIDYAQTKMERKGIQMICANDVSRADIGFGSDHNELTVFYRAEQELETYKLGPKLKTELAQDLVELVNTIRK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.2. The protein should be able to bind CTP ligand in a variety of conditions3. Ideal protein should have a binding affinity for key enzymes in the CoA from (R)- pantothenate: step 3/5 pathway to enhance the overall efficiency of the pathway.4. The protein must have high specificity towards metal ion binding. The designed protein sequence is ",
    "response#1": "GSNNDIKVKAARLVFRRIFKGMRVRVVGIGSESGVPEQPFGRDTIRGAENRARVAVAEGDYGVGIEAGLIWNEHMQQYLDVQYCAVVDKIGRVTYGHGSGFYYPPKVIEKVVEGKRKHVTISDAMKDTVGIEDIGKKQGAIGYLSKGMMTRTKLTEQAVLMAMIPRR",
    "response#2": "MRILLAITSSISAYKTPFLVSHLKKQGHNVICAVTKNAQNMVGAKALETMSGNKVITDLWQEDDILRHIHINEETDILLIAPADVNIIGKIANGICDDAISTIACAYTKKKYFAPAMNPNMWANKANQRNVKYLIEELNYELISPESGEMACNDTGVGRLADIDTIIQKVLAFLKE",
    "response#3": "MRILLAITSSISAYKTPFLVSHLKKQGHNVICAVTKNAQNMVGAKALETMSGNKVITDLWQEDDILRHIHINEETDILLIAPADVNIIGKIANGICDDAISTIACAYTKKKYFAPAMNPNMWANKANQRNVKYLIEELNYELISPESGEMACNDTGVGRLADIDTIIQKVLMWLEK"
  },
  {
    "reference": "MPSLRAHVFRVPADGPDDVAGVEALFASGLQANNVVAVLGKTEGNGCVNDFTRGYATRSFETLFSRYGVDGVSIIMSGGTEGALSPHWTVFARETVETPGERALAIGVSRTPALPPEHLGRREQILLVAEGVKSAMRDAGIDDPADVHFVQIKCPLLTSRRIAEAEAAGRTVATHDTLKSMGLSRGASALGVAVALGEIDATSIGDADICTRFDLFSRCASTSSGGELTDHEIIVLGMSAKWSGPLSIDHAVMLDAIDAHSVRKARERLPENSRLAAVLAKAEPDPSGRIDGRRHTMLDDSDIAGTRHARAFVGGVLAGIFGITDLYVSGGAEHQGPPGGGPVAIIVEKEQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should interact with enzymes and substrates within the biuret from cyanurate: step 1/1, atrazine degradation pathway to enhance or regulate its activity.2. For general function, the protein need meet that Catalyzes the hydrolytic opening of the s-triazine ring of cyanuric acid (2,4,6- trihydroxy-s-triazine) to yield carbon dioxide and carboxybiuret, which spontaneously decarboxylates to biuret.3. The designed protein must possess metal ion binding to support atrazine catabolic process under certain conditions. The designed protein sequence is ",
    "response#1": "MXAMVVALTMXRL",
    "response#2": "MSISLRL",
    "response#3": "MNRKTLIVVSAGLPYANGSLHLGHLLESIQADVWARYQKLRGNEVLYVC"
  },
  {
    "reference": "MFLEIILDSATYSFDRVITGKICFETNAATSPRSFQIQLRIKGYAVYHHGFLSSPSHKHPTTASASDSDEATEIFTHSQPLPIPAGPSSKSHAIDFKFKFPSKSASSLPCSKSNDSMVNICYMLKATISKRYAFLGSSQSAKAELIMVPPNLAGKPLSANSSFGSSAKTFQLPEFDSVTPSASLYKQPSFNSNPAPITTSSATHTSQFSTSSSSSVNSVHTPVMVPNPYFQYNSSMTAPSSSSSSVAPFVPRRQFSVSSASDPPQTPISMSPPIPPTPSQFSAFMYQNQQSYLSPQSHYENPLSISSRPSPCCPSTPSSAVTLSNGFDSQSNAYNNSGTGPPMLYKFPQRSYTAPNTSFNSQRRMSSITSLPTASFCPVKHGVSPPSLAGNQPSPLSSPLTNSNVSPSTICSPDNNVTFVNLAPGEKLTFKIEPNDLMDEEEVVENSNMYSIPGKTVATTVCHSSSSSGDFSALSCHRNLSFSESLPTAEDQVHNNHCAVSPSRRSSSNALYLATLPMGYERSNSVSYCSDSSLSSPLPDDNMLQDPHALNMAYAKEFDVLINEVLQSL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein localizes to the nucleus.2. The protein must exhibit the following characteristics: Has a role in promoting meiosis whereby it is involved in establishing the mating pheromone signaling pathway. The designed protein sequence is ",
    "response#1": "PMEFKGTSKDDLEDDLNSFFDCEEELFLSANKIFYSKLRVYQTVRHWSLKQNWSSMSLDQKRNFILHCHHELSSGELLRNSALYSILYIAAGAYDSVGSFEEHVHSISENVYLLREFDIPKTVYSLFIEWNRTRKHSFLDNSRDEGFLNLLLSLMFFFLTLNNTDKTWIHSIRSLNPPVLPTLVKLFIDITSDYQLNLDKVIHSSLKKLSFLIFKVCIRLWGSQSYLQEKKAGLAEVLNISTSQQKPKTTALDYEVFRHEMATKFSSFADSYYPVPLDRL",
    "response#2": "HKPMEFKGTSKDDLEDDLNSFFDCEEELFLSANKIFYSKLRVYQTVRHWSLKQNWSSMSLDQKRNFILHCHHELSSGELLRNSALYSILYIAAGAYDSVGSFEEHVHSISENVYLLREFDIPKTVYSLFIEWNRTRKHSFLDNSRDEGFLNLLLSLMFFFLTLNNTDKTWIHSIRSLNPPVLPTLVKLFIDITSDYQLNLDKVIHSSLKKLSFLIFKVCIRLWGSQSYLQEKKAGLAEVLNISTSQQKPKTTALDYEVFRHEMATKFSSFADSYYPVPLDREL",
    "response#3": "PMEFKGTSKDDLEDDLNSFFDCEEELFLSANKIFYSKLRVYQTVRHWSLKQNWSSMSLDQKRNFILHCHHELSSGELLRNSALYSILYIAAGAYDSVGSFEEHVHSISENVYLLREFDIPKTVYSLFIEWNRTRKHSFLDNSRDEGFLNLLLSLMFFFLTLNNTDKTWIHSIRSLNPPVLPTLVKLFIDITSDYQLNLDKVIHSSLKKLSFLIFKVCIRLWGSQSYLQEKKAGLAEVLNISTSQQKPKTTALDYEVFRHEMATKFSSFADSYYPVPLDRM"
  },
  {
    "reference": "MLRYLTAGESHGRGLSVIVEGLPAGVPLTDGDINTWLTRRQGGYGRGGRMAIERDQAEILAGVRGGLTLGSPIALFIANRDWENWQEIMAPGPEARAARVVTRPRPGHADLAGGLKYHQADLRNILERASARETAARVAAGAVAAVLLKELAIELAFHVVRIGPVEVREQVDWEAACRAVESPVYCADPEAGRAMVAAIEEARQQGDTLGGVVEVLARGVPAGLGSHVHWDRRLDGRLAQALMSIPAIKGVEIGAGFRVAALPGSRAHDAIAYRKGQGFYHPTNRAGGLEGGLTNGETLVLRAAMKPIPTLMHPLPSVDLVTKQPATASIERSDVCAVPAAAVVAAAAVAWVLAGAILEQFGGDYLPVIQERLAAYRQYLQEI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The FMN binding site should be stable and able to withstand changes in environmental conditions.2. The protein should be optimized for activity in chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7, Metabolic intermediate biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The designed protein must possess chorismate synthase activity to support aromatic amino acid family biosynthetic process, chorismate biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "SFVTGIV",
    "response#2": "MLNIVMGRSSARETAVRVAAGAC",
    "response#3": "DXCLXFX"
  },
  {
    "reference": "MFQWLLQCQAPRLPKAGETIHGHRFFTGFGGKGANQCIQAAKLGARTAMVGKVGKDFFGDSYIQNFKDNGVNTACVFQAPDVATGAASIVVNDAGENAIVIVAGANLLLGAAELQASLPALSRTTVLLCQLEIRPECSLQALRTARQHNVKTIFNPAPAVPDLDPDFYTLSDVLCCNESEAELLTGSPVATVEDARRASGELLGRGCGAVLLTMGAQGCVVLEAKGAATHHVPAPAVTAVDTTGAGDSFLGALAFYMAHYPALPLEEMARRANHIAGVSVQAVGTQASYPSRTDLPAELF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that can perform metal ion binding, ribokinase activity in nucleus, cytoplasm to support nucleoside phosphate metabolic process.2. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.3. The protein should be optimized for activity in D-ribose degradation, Carbohydrate metabolism, D-ribose 5- phosphate from beta-D-ribopyranose: step 2/2 pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The K(+), ATP, substrate binding site should be located in a region of the protein that is accessible to the ligand.5. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for ATP + D-ribose = ADP + D-ribose 5-phosphate + H(+).6. The protein must exhibit the following characteristics: The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.; Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium.7. The designed protein must have at least one Carbohydrate kinase PfkB domain. The designed protein sequence is ",
    "response#1": "MKIFSLNSNRALAEKIANEVGIPLGKATIKQFSDGEIQVNIEESIRGAEVYVIQSISDPINDSLMELLIMVDALRRASARQINVVIPYYGYSRQDRKARSREPITAKLIANLLAAPLLASYFQEHNFDSENLVVVSPDHASVSRARNMAELLGTPIAIIDNRNEESAENLNEIPTEIIGDVKGKVALVIDDMIDTGTRLTISAEALHNAGATEIYGAATHAIFSRNAPQILQASKLSKVIVTDALPLFARYFANKGIKDAVVVAPDTGRAKMAKKFSDKIGAQLAILHKSRPEHNVADIMHVVGDVKDKTVILFDDMVDTGGSVTQGLKVLREHGANPEIYLATTHPVFSGPAIERLTNANFKEVVTTNAAPLLASYFQEHNFDSENLVVVSPDHASVSRARNMAELLGTPIAIIDNRNEESAENLNEIPTEIIGDVKGKVALVIDDMIDTGTRLTISAEALHNAGATEIYGAATHAIFSRNAPQILQASKLSKVIVTDIPVSLLGETIHGHKFFIGFGGKGANQCVQAARMGAKTAMVCKLGNDFFGDKYVQNFKDHGISTDYVEQTAEAVTGAASIIVNDAGENAIVIVAGANLLLGNEEIQRAKPAVSQAKVLVCQLEISPDASLKALHMAKESYVRTIFNPAPAIADLHSDFYKASDVFCCNESEAELLTGQTVATVEDAGKVGVELLGRGCGSVIITLGAQGCVVITAQGSSPKHVPTSKVTAVDTTGAGDSFIGALAFYIAHYPTMPLEEMARRANLVAAVSVQTEGTQSSY",
    "response#2": "MNTVTVIGSINLDRTIRVENMPKPGETIHTKEIFSAGGGKGANQAVAAQRSGANTHFIGAVGDDAAGKTMLDLLTQEKINLAGITKMTNQSTGQAYVTVDDAGENQIMIHGGANMAFTPADVEAHRDIIETSDFVVAQFESAVDSTVEAFKIAQAAGVRTILNPAPAMEKVPAELLAVTDMIVPNETETETLTGIAITDEASMLKASAALHALGISAVIITIGSKGAFYDIDGRHGIVPAFKVEAVDTTSAGDTFIGAMSSVLNKDFSNLEDAIRYGNRASSIAVQRFGAQPSIP",
    "response#3": "MNTVTVIGSINLDRTIRVENMPKPGETIHTKEIFSAGGGKGANQAVAAQRSGANTHFIGAVGDDAAGKTMLDLLTQEKINLAGITKMTNQSTGQAYVTVDDAGENQIMIHGGANMAFTPADVEAHRDIIETSDFVVAQFESAVDSTVEAFKIAQAAGVRTILNPAPAMEKVPAELLAVTDMIVPNETETETLTGIAITDEASMLKASAALHALGISAVIITIGSKGAFYDIDGRHGIVPAFKVEAVDTTSAGDTFIGAMSSVLNKDFSNLEDAIRYGNRASSIAVQRFGAQPSIPYIQPTL"
  },
  {
    "reference": "MIEFLIGLIAAVVGILVGYLIARKINNANYEIFLEQAKAKAKAIEFEAEGILRNSKISVQEAEFEAKKAYEDKALKLQKDYNAKFDEISKKEQTVLTEQEILKDSREELEKEKKSAQTIYDEGTSLKKTYETKVEESLKLLERVAGLTEDEAKSEILQKVEEKSRAEIAHIVRKYEEEAKKEAKRNANYILAQATTRYAGEYAAERLINVVNIKNDDLKGRIIGKDGRNIKTLEMISGVDVIIDDTPNAIILSSHNLYRRAIAVRTVELLVEDGRIQPARIEDVYKKVSEEFEAGIQEEGENIVMDLGLTKIHPEIVKLIGKLKFRASYGQNALIHSLEVAHLAGIIAAETGGDENLARRAGILHDIGKALTHEFEGSHVDLGAEICKRYKENPVVINAIYAHHGHEEPTSVESAAVCTADVLSAARPGARREVLEAFLKRVSEIENIATSKEGVKQAYAINAGREIRVIANAKLVNDDEAVLLAKEIAEEIQAKVQYPGEIKVNIIRETRAVDYAK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Endoribonuclease that initiates mRNA decay..2. The designed protein should be involved in mRNA catabolic process by localizing to plasma membrane and exhibiting endoribonuclease activity, RNA binding.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.4. The designed protein must have at least one KH, HD domain. The designed protein sequence is ",
    "response#1": "SLAGRYLVLMPNNPRAGGVSRRIEGDDRAELREALAELNIPEGMGVIARTAGVGRTPEELQWDLDYMTALWSAILQASTEADAPALIHQESNVIIRALRDHMRNDVGEVIIDDEMVYRQALEFVERVMPNSLRKIKLYSDPVPLFNRFQVESQIESAFERLVALPSGGALVIDHTEALISIDVNSARATKGGDIEETALTTNLEAAEEAARQLRIRDLGGLIVIDFIDMTSNKHQREVETRLRKALEMDRARVQVGRISRFGLLEMSRQR",
    "response#2": "TCPRCQGHGQIRSVESLALSILRLIEEEAMKERTSLVLAKVPVDVGTFLLNEKRESLTDIEDRHDVDVSILPAPQLLTPHFELRA",
    "response#3": "VLKHSIEVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEVIHAMSTHHGDYEPQTVEAVLVTAADAISY"
  },
  {
    "reference": "MVIQRKYRASRRLGVSLWGRSKDPFNTRNYPPGQHGNMGYKKPSDFGKQFGAHKKFKFYYAISSKQMRNMFLKAYKKKGDTGDNFVGLLESMLSSVLYNSGLVPTIFSARQLISHKHVLVNGKVVNISSYSVKPGDTIKLREKAVNLPPVLAAIDAQEQKVPDYLEVDVKERSVKYLRVPKYYEVPYPANMEVNLVIEFYSR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that can perform structural constituent of ribosome, rRNA binding in small ribosomal subunit to support translation.2. The protein contains novel S4 RNA-binding domains that confer a unique function or activity.3. For general function, the protein need meet that With S5 and S12 plays an important role in translational accuracy. The designed protein sequence is ",
    "response#1": "MHRRLDAVVYRAKFVSTMFAARQFINHGHIKVNGKRVNIPSYKVRVGDVIEVKEASKQLAFVLEASR",
    "response#2": "MRLDNIVFRLHMAPTIKAARQLISHGHILINKKKVTIPSYQCEPKDIITVAPKIISMELVSRFLK",
    "response#3": "MRLDNIVFRLHMAPTIKAARQLISHGHILINKKKVTIPSYQCEPKDIITVAPKIISMELVSRFLN"
  },
  {
    "reference": "MAHASDLISDDIEAYLKEHERKDLLRFITCGSVDDGKSTLIGRLLHDSKMIYEDQLAAITQASKTAGTTGDEVDLALLVDGLQSEREQGITIDVAYRFFSTDRRKFIIADTPGHEQYTRNMATGASTASLAVILVDARHGVQTQTRRHSFIADLLGIQHLVVAINKMDLVDYSQERYEEIVAEYQEIAGKLNAPDIRFVPLSALKGDNVVNPSEAMDWYRVDGKQGPALLELLETVEITHDQNLRDLRLPVQYVNRPNLDFRGYAGTLAAGVMSPGQKVRALPSGKVSTVERLVTWDGDLEAAYPGDAITVTLEDEIDISRGDWLVAENDEVPLATSFEADIVWMHETALEPGKLYDIKLATRDVAGKVAAIDYQVDVNTLEHRHADHLDLNAIARCALELTAPVPVDDYRTSPGTGSFIIIDRLTNITVGAGMIHRPLESELPYEFREHDSKASVVWNRTSVTQAMREQLNGHAGKCVWFTGLSGSGKSSLANALEVELNRRGYHTMLLDGDNIRHGLCKDLGMGEADRTENIRRVGEVARLFAEAGIIVITAFISPFRADRDAARALFDEGDFIEVHVDTPLDICEQRDPKGLYQKAREGRIADFTGISSPYEAPPQAEVTLKGAEHGQQELLAQLIKRVI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein have Tr-type G domains with specific sequence motifs that are crucial for its activity.2. The Phosphoserine intermediate of the protein must be engineered to enable effective catalysis of adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate   + ADP + H(+).3. The protein must exhibit the following characteristics: With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway.4. The protein design should take into account the physiological conditions of the sulfite from sulfate: step 2/3, Sulfur metabolism, hydrogen sulfide biosynthesis pathway.5. The protein should have a high affinity for ATP, GTP.6. The designed protein should be optimized for ATP binding, GTPase activity, adenylylsulfate kinase activity, sulfate adenylyltransferase (ATP) activity, GTP binding activity in order to improve hydrogen sulfide biosynthetic process, sulfate assimilation, phosphorylation. The designed protein sequence is ",
    "response#1": "MTTLRFLTCGSVDDGKSTLIGRLLFDAQLIFDDQLAALEADSKRHSTQGEAVDFALLVDGLAAEREQGITIDVAYRYFATAKRKFVVADTPGHEQYTRNMASGASHSDLAVILVDARKGVLTQTRRHSAIASLFGIRHFVLAVNKMDLVGYDQGVFDAIEAEYRAFAADLGDISVHALPISAWKGDNVTEPSEAMPWHQGPALLPFLEEVVVEPFRFPVQWVNRPDADFRGLSGTVASGRVAEGDRVVVAASGRAGLVSRIVTADGDLSAAAAGDAVTLVLEGDLDVARGDLIARRGNPPAVHLLWMSEDDLIPGRQYRLKSATRTLDCVVTELKHAVNINTLETQAAKKLALNEVGVANISLSERLAFDPFQDSAPMGGFILMDRRSNATVGVGMLNFSLRRADNVHWQSLDVDRDVHAALKGQRPCLLWFTGISGAGKSTIANLVEKKLHALGRHTYLLDGDNVRHGLNKDLGFTDADRVENIRRVGEVAKLMADAGLIVLTAFISPFRAERDMVRAMMPEGDFVEVFVDTPLAVAEQRDVKGLYRKAREGKLKNFTGIDSPYEPPEHAEIRIDTTALSAEAAAERIVAQLMERGVIA",
    "response#2": "MTTLRFLTCGSVDDGKSTLIGRLLFDAQLIFDDQLAALEADSKRHSTQGEAVDFALLVDGLAAEREQGITIDVAYRYFATAKRKFVVADTPGHEQYTRNMASGASHSDLAVILVDARKGVLTQTRRHSAIASLFGIRHFVLAVNKMDLVGYDQGVFDAIEAEYRAFAADLGDISVHALPISAWKGDNVTEPSEAMPWHQGPALLPFLEEVVVEPEISAHLIWMDSAHLFVGRPYIIKLGSQQVQATITALNHKIDVNDASRKDAGKTLELNEIGFCNIAFSQPLAMDAYEENRQTGSFILIDRYSNQTVAAGLQFEATLVWFDEDALLPGRPYLMKLGTRTATAQVAEPKYKVNVNTLEQLAAKRLELNEIGVCNLALSAPIAFDPYADNKDLGGFILIDRMSNRTVGAGMISAHLIWMDSAHLFVGRPYIIKLGSQQVQATITALNHKIDVNDASRKDAGKTLELNEIGFCNIAFSQPLAMDAYEENRQTGSFILIDRYSNQTVAAGLQFEATIVWMADEEMLPGRSYWLKIGTQTVSASVQAPKYQVNVNTMEHLAAKTLDLNAIGVANLSTDKPVVFEPYADNRALGGFILIDKISNATVAAGMINFALRRSQNVHWQAVDITRAAHAGQKGQTPRVLWFTGLSGSGKSTIANLVEKKLHALGKHSFLLDGDNVRHGLNRDLGFTDADRVENIRRVAEVAKLMADAGLIVLTAFISPFRAEREMVRAMMQPGEFMEIFIDTPLEVAEQRDVKGLYAKAR",
    "response#3": "MTTLRFLTCGSVDDGKSTLIGRLLFDAQLIFDDQLAALEADSKRHSTQGEAVDFALLVDGLAAEREQGITIDVAYRYFATAKRKFVVADTPGHEQYTRNMASGASHSDLAVILVDARKGVLTQTRRHSAIASLFGIRHFVLAVNKMDLVGYDQGVFDAIEAEYRAFAADLGDISVHALPISAWKGDNVTEPSEAMPWHQGPALLPFLEEVVVEPHAARAPRFPVQYVNRPNLNFRGFCGTVASGIFKPGQAIKALPSNKTSKVKAIITYDGELSEALPGDAVTLTLEDEIDISRGDMIVRPGNEIADRFSAHLIWMGDQKLVPGRTYAVKAGKQSATGVVSRIGHRIDVNTFAQDEVDSLSLNEIGFCNLALSAPIAFDPYEVNREMGSFILIDRMTNQTVAAGVVGRDIDAMVCWFSEDTELTAGNRYQMLCGTRLTRAQVSGLNYRLDVNTLHRDEESTALRLNEIARLSVHTQQPVMFDSYRKNRETGSFILIDEATNKTVAAGMILDRSAAAGAAVHRQTVAVTAADRAAHKGQQARLLWFTGLSGSGKSTLANEVERMLVLAGRHTMLLDGDNVRRGLNKDLGFTEADRVENIRRVAEVARLMADAGLIVLVSFISPFRAERAAARELFGADRFIEVFVDTPVAECARRDPKGLYRKAAGGELPNMTGIDSPYEAPENPELRLDTTTHEPDEAAAQIVAWLRGE"
  },
  {
    "reference": "MNIAVSKIESEASLASAPNQIVRPQVRIVIVGHVDHGKSTLVGRLLHETGSLPDGKLEMLQAVSARRGMPFEWSFLLDALQTERDQGITIDTTQIRFRTPSRDVVLIDAPGHAEFLRNMITGASQADGAILIIDALEGVRDQTRRHGYLLHLLGVKQVAVVVNKMDRVDYSAARFAEISDEISAHLTGLGVTPTAVIPISARDGDGVTQRTANIGWYQGPTVVEAIDHLTPARPLDELSLRLPVQAIYKFDDRRIVAGRVESGDIGTGDEIVIMPTGKVAKVKSVESWPQTPLHVRQGAGRSVGITLDRELFLERGDIIAHVKAAPRDTRRLRARIFWLHDAPLKAGASVLVRLGTMESRATIVAIDKAVDPGELESVASAAIGRNHVGEIDIALAKPVATDPYTENPRTGRLVIEVNGRIAGGGLVLSVDSGQRAIPVDIVPVESALRSDERSARYGHGGAVVWLTGLPGSGKSTLARALERKLFSRGGSPLLLDGDTLRAGLNGDLGFSPKDRAENIRRLAEVATHLARNGHIAIVAAVSPSSEDRAQARHIADDLFREVYVATPAEVCESRDPKGHYAKARAGDLPSFTGIANDYQPPANAELIIDTSARSVADGAEAIERLLAETGVLFGDLNDLAANI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must possess ATP binding, sulfate adenylyltransferase (ATP) activity, adenylylsulfate kinase activity, GTPase activity, GTP binding to support phosphorylation, hydrogen sulfide biosynthetic process, sulfate assimilation under certain conditions.2. The designed protein have Tr-type G domains with specific sequence motifs that are crucial for its activity.3. A protein should be able to manipulate the activity of enzymes in the Sulfur metabolism, hydrogen sulfide biosynthesis, sulfite from sulfate: step 2/3 pathway, either by activating or inhibiting their function.4. A key design requirement for the protein is the optimization of the Phosphoserine intermediate to achieve high catalytic efficiency for adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate   + ADP + H(+).5. For general function, the protein need meet that ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity.; Proposed to provide activated sulfate for transfer to Nod factor The designed protein sequence is ",
    "response#1": "MAPMKLVIVGHVDHGKSTLIGRMLHDTGSLPDGKMEAVKAMSDRRGMPMEWAFVLDALQAERDQGITIDTTQIFFKTPARPYVIIDAPGHKEFLKNMVSGAASADAAILVVDAKEGVQEQTRRHGYILHLLGLDQVVVAVNKMDLVDHSQARFDEVAGEVHKYLGSMGITPAAILPVSARHGDGLAVKSTNMPWFEGETLIASLDTLPPKPFRMPVQWINRPDLDFRGYCGTIVSGRICPGDPVAVAASGQHTRIARIVTMDGDVEEAQSGDAVTLTLADEVDASRGDVIEIADRFSAHLIWMGDQKLVPGRTYAVKAGKQSATGVVSRIGHRIDVNTFAQDEVDSLSLNEIGFCNLALSAPIAFDPYEVNREMGSFILIDRMTNQTVAAGVLRARIFWLHDQPLTAGSSILVRLGVREVRGRVVAIEKAIDPGELASVETKQIARNHVGEIEIALSQPVAADRYVDNPRTGRLVIEVGGRIAGGGLVRHALHRAKSINLSAVDVDLAARTRRLRARIFWLHDQPLTAGSSILVRLGVREVRGRVVAIEKAIDPGELASVETKQIARNHVGEIEIALSQPVAADRYVDNPRTGRLVIEVGGRIAGGGLV",
    "response#2": "MAPMKLVIVGHVDHGKSTLIGRMLHDTGSLPDGKMEAVKAMSDRRGMPMEWAFVLDALQAERDQGITIDTTQIFFKTPARPYVIIDAPGHKEFLKNMVSGAASADAAILVVDAKEGVQEQTRRHGYILHLLGLDQVVVAVNKMDLVDHSQARFDEVAGEVHKYLGSMGITPAAILPVSARHGDGLAVKSTNMPWFEGETLIASLDTLPPKPLSNVFRMHLFWLSDKPLKVGNVYKIRLATQEALATVQAIEKTIDTDDLKSSEMGDVVRNGVAEIVFRTRDMLALDPFSDNPSTGRCVIYDGFDVAGGGIFKARLFWLGHEPLTAGKSYKLKLGTFDTPVTVQKVDRIIDTSDLSNSESEQVERNQVAEVTIRSRRMLALDEFTSNGRTGRFVLVDKYDIAGGGILRARIFWLHDTPLTAGASILVRLGTMETRANVVAIDKAVDPGELESVATASIGRNHVGEIDISLAKPVAADAYTDNPRTGRLVIEVNGRIAGGGLV",
    "response#3": "MAPMKLVIVGHVDHGKSTLIGRMLHDTGSLPDGKMEAVKAMSDRRGMPMEWAFVLDALQAERDQGITIDTTQIFFKTPARPYVIIDAPGHKEFLKNMVSGAASADAAILVVDAKEGVQEQTRRHGYILHLLGLDQVVVAVNKMDLVDHSQARFDEVAGEVHKYLGSMGITPAAILPVSARHGDGLAVKSTNMPWFEGETLIASLDTLPPKPFRMPVQWINRPDLDFRGYCGTIVSGRICPGDPVAVAASGQHTRIARIVTMDGDVEEAQSGDAVTLTLADEVDASRGDVIATSRMRARIFWLHDKPLTAGASILVRLGTRETRATVVAIEKAVDPGELTSVETKSIARNHVGEIDIALAHPTAADPYTDNPRTGRLVIEVNGRIAGGGLLRARIFWLHDQPLTAGSSILVRLGVREVRGRVVAIEKAIDPGELASVETKQIARNHVGEIEIALSQPVAADRYVDNPRTGRLVIEVGGRIAGGGLVLG"
  },
  {
    "reference": "MDFLPLFCQLRGKACLLIGGGEVAERKARLLLDAGADLRVCSREFAPQFLHWRQTHSVTLIYDAFTPELLDDCWLVIAATNDEALNQQVSAEAESRRIFCNVVDAPESASVIMPSIIDRSPLMIAVSSGGRAPVLARLLREKLEAMLPQHLGKLADFAGALRQRVKATLPSPALRRRFWEKLFSSDRLAQSLANDDQQRVEQLKETLFSEPLEHRGEVVLVGAGPGDAGLLTLKGLQQIQQADIVVYDRLVSDEIMNLTRRDAERIFVGKRAGFHCVPQEEINQILLQQAQRGKRVVRLKGGDPFIFGRGAEELEALLGADIPFSVVPGITAASGCSAYSGIPLTHRDYAQSVRLVTGHLQQNGALEWSRMAADHQTLVFYMGLSQAAEIQHQLITHGMRTTMPVALVENGTTVNQRVVSGELTQLASLATQVKSPSLIIVGEVVALREKLRWF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be able to regulate methylation. The designed protein sequence is ",
    "response#1": "VTLPSHSTRKLQTRSQTWLESREYTKHLIRVENWIFRNXXXXXXXXAIAWLLGSSTSQKVIYLVMILLIAPAYMA",
    "response#2": "VTLPSHSTRKLQTRSQTWLESREYTKHLIRVENWIFRNXXXXXXXXAIAWLLGSSTSQKVIYLVMILLIAPAYS",
    "response#3": "ESPLHIHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWCAEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITALR"
  },
  {
    "reference": "MAIGVVGRKAGMTRIFTDEGQALPVTVIEVEPNRITQLKTLESDGYRAVQVTVGSRRSSRVNKAAAGHFAKAGVEAGRGVWEFRLADGEGEDLAAGGELTVSVFEDGQVVDAVGQSKGKGFQGGVKRWNFSMQDATHGNSLSHRAPGSIGQNQTPGKVFKGKKMAGQMGNAQVTVQNLKIVRVDAERNLLLVSGAVPGATGGDVVIKPAVKA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that can perform rRNA binding, structural constituent of ribosome in ribonucleoprotein complex, ribosome to support translation. The designed protein sequence is ",
    "response#1": "RPMTEEESKTFFEKLIKFIGKNVTLLIQRKDEPYCFRIQKNKVYYVSEELMKRSQNIPREGLCSLGTCFGRFTKTGKFKLNITCLDK",
    "response#2": "RPMTEEESKTFFEKLIKFIGKNVTLLIQRKDEPYCFRIQKNKVYYVSEELMKRSQNIPREGLCSLGTCFGRFTKTGKFKLNITCLDR",
    "response#3": "RPMTEEESKTFFEKLIKFIGKNVTLLIQRKDEPYCFRIQKNKVYYVSEELMKRSQNIPREGLCSLGTCFGRFTKTGKFKLNITCLDC"
  },
  {
    "reference": "MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSHIVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGAHIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLSHDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHDGIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENAAGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISGAEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPCIERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIKVVCG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design must be able to perform 4 iron, 4 sulfur cluster binding, L-serine ammonia-lyase activity, metal ion binding in order to contribute to gluconeogenesis, amino acid catabolic process.2. The protein have enhanced L-serine = NH4(+) + pyruvate.3. The stability of the protein-[4Fe-4S] cluster complex should be optimized to maximize enzymatic activity.4. We require a protein that can specifically bind to the metabolites produced in the Amino-acid degradation pathway. The designed protein sequence is ",
    "response#1": "IKGDFLNFGNDCLSNMVNKTTSACNTRMIGENMPAMSVAKSGNMGIMATLPIIAYDYSNEQNQEKLIKSILLSVLVTIYATYKSSYLSSMCGCVSKGGMGAVIGLCYYKNGKNIKKLDSAARTFTANLPGIICDGGKVGCALKLASGCFAAYSSLFVDISYENGIVGKNFKECVENISEIK",
    "response#2": "IKGDFLNFGNDCLSNMVNKTTSACNTRMIGENMPAMSVAKSGNMGIMATLPIIAYDYSNEQNQEKLIKSILLSVLVTIYATYKSSYLSSMCGCVSKGGMGAVIGLCYYKNGKNIKKLDSAARTFTANLPGIICDGGKVGCALKLASGCFAAYSSLFVDISYENGIVGKNFKECVENISEIL",
    "response#3": "LHLVHEVTSPQAFDGLRLAGRRVRRPELTLATEDHNVPTVDIDQPIADPVSRTQVETLRRNCAEFGIRLHSMGDIEQGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGALAMGIGTSEVEHVLATQTLPLRPFKTMAVNVDGRLPDGVSAKDIILALIAKIGTGGGQGHVIEYRGSAIESLSMEGRMTICNMSIEAGARAGMVAPDETTYAFLRGRPHAPTGAQWDTALVYWQRLRTDVGAVFDTEVYLDAASLSPFVTWGTNPGQGVPLAAAVPDPQLMTDDAERQAAEKALAYMDLRPGTAMRDIAVDAVFVGSCTNGRIEDLRVVAEVLRGRKVADGVRMLIVPGSMRVRAQAEAEGLGEIFTDAGAQWRQAGCSMCLGMNPDQLASGERCAATSNRNFEGRQGAGGRTHLVSPAVAAATAVRGVVTDVRTLL"
  },
  {
    "reference": "MARSRSRSPRWKHRSLSPVPRNAEHYKQRHSHGHYGCEYRKDPKRPVAWRMDSEKHGQSKPRIPSRGNIYYQSYEHRSPSPNIRNSLENVYMYKPHRGYSPGRGDSNRRAQYMPKYSEGIPYKEHERNSYPQKVQGGHSPDDHRVRGSGKGGKPPQRSIADSFRFEGKWHEDELRHQRIQEEKYSQSTRRGSEDFETRSSFQKRYPEDRDFRKYGHTSKRPKDVERYESREPARNPKWKPEHSLPPYQEDTDQWNLGPQTYRHAEREHPETSSATKVSYDYRHKRPKLLDGDQDFSDGRTQKYCKEEDRKYSFQKGPLNRELDCFNTGRGRETQDGQVKEPFKPSKKDSIACTYSNKNDVDLRSSNDKWKEKIKKEGDCRKESNSSSNQLDKSQKLPDVKPSPINLRKKSLTVKVDVKKTVDTFRVASSYSTERQMSHDLVAVGRKSENFHPVFEHLDSTQNTENKPTGEFAQEIITIIHQVKANYFPSPGITLHERFSTMQDIHKADVNEIPLNSDPEIHRRIDMSLAELQSKQAVIYESEQTLIKIIDPNDLRHDIERRRKERLQNEDEHIFHIASAAERDDQNSSFSKVKNVHTDGFQKPTHFIKSNFRKCIEKPYMNYTTQRKDIITHKPFEVEGNHRNTRVRPFKSNFRGGRCQPNYKSGLVQKSLYIQAKYQRLRFTGPRGFITHKFRERLMRKKKEYTDVATGI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should exhibit Basic and acidic residues compositional bias.2. The protein should have DNA binding and be active in mediator complex, mitochondrion. The designed protein sequence is ",
    "response#1": "DPGFQDLWNSSYAGLTLRLAGNLPNGLHEKVQSALVTLQRCGCLLRDLVRVRDRDVFTTVSRILVGQPGYTYRYLDTRLFTIPWHCDGEGQKDEKPCCDAHLMNACKALWELNEFFCSDVKEQTNTRGVKRTHSDSVNSEDMAGDGMCEESAKDRLAEEKDQDDEDSGQGCSHSSPLPVANPGLVQFNVTLINYMDPTAMTQLKEEPYYGMGKMAVGWHHDENLVPLSPVAVYSYSCPAEPKNEGMAEKEKQSKGKELQKVGEGTSKEVKEAGEASGKEEMEKERSCWRVGLKVAWDIHTPGLALPLQSGDCYYLTDDLNRTHQHCVLAGDTARFSSTHRVVQC",
    "response#2": "DPGFQDLWNSSYAGLTLRLAGNLPNGLHEKVQSALVTLQRCGCLLRDLVRVRDRDVFTTVSRILVGQPGYTYRYLDTRLFTIPWHCDGEGQKDEKPCCDAHLMNACKALWELNEFFCSDVKEQTNTRGVKRTHSDSVNSEDMAGDGMCEESAKDRLAEEKDQDDEDSGQGCSHSSPLPVANPGLVQFNVTLINYMDPTAMTQLKEEPYYGMGKMAVGWHHDENLVPLSPVAVYSYSCPAEPKNEGMAEKEKQSKGKELQKVGEGTSKEVKEAGEASGKEEMEKERSCWRVGLKVAWDIHTPGLALPLQSGDCYYLTDDLNRTHQHCVLAGDTARFSSTHRVVQC",
    "response#3": "GGQFAVADAKKPIGGNIMAHASTTRLNLRKGRGTSRVCKIVDSPCLPEAEAIFAINPNGIGDPEC"
  },
  {
    "reference": "MALKLEPGAKLVVATHNPGKARELAEILENRFQLVTAGDLALPEPDETEMTFVGNALLKARAAADASGLVALADDSGLSVAALDGAPGIYSARWAGPGRDFADAMKKVEQRLEEAETEDLAAWFTCALAVAWPDGPAVVVEGRVDGALAFPPRGDRGFGYDPIFVPDGRTETFGEMDPQAKDAMSHRARAFAKLKAALL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design must be able to perform dITP diphosphatase activity, XTP diphosphatase activity, ribonucleoside triphosphate phosphatase activity in order to contribute to purine nucleoside triphosphate catabolic process, nucleotide metabolic process.2. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.3. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..4. We are looking for a protein with broad substrate specificity for dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MVILSGTSNSGKTTLAKQLQNKFPQLTIDHLDELRPGLKWQPRGNYKTIEGWDYDVVEGWAKDVIAKEKQNCVIIDFGAGSNTRWQRAKNAIVIYLNLPFEQSRQRVKERGQHMGWEGKEWDQYIYGYTWGLDYGNHMDERFDVEICTDNFNAEQIIQKAEEYLG",
    "response#2": "MXRXVXATGLXTGXGXA",
    "response#3": "MGTFIFSIVLALIIWGISLFFSNKK"
  },
  {
    "reference": "MKTIEIIGYRRANLGKQASKQLRNDAQVPGVLYGGKEQVHFYTPMAILKDLVYTPQAYFVTLNLEGAIYNCILQDIQFHPVNEVILHIDLLQIFEDKQIKMDIPIVLVGDAPGVVKGGNLVHKKKKLSVVAYPKDMPDTIPVDISGLDVGQMTRVSHIKAENYTILAVPGTPVAVVETTRALRAGAAEAEGKGKKK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be able to regulate translation. The designed protein sequence is ",
    "response#1": "TLKIMQVNENADENSLREELLFPFAPIPRVSVVRNKETGKSRGLAFVTFSSEEVAEQALRFLDGRGYMNLILRVEWS",
    "response#2": "DGSRSIITYKIEDGVKYKITQKVKEVKVLEKVHKSVAERKNWHKYGSEKGSPAGPSAVTARLGEEVELRLSRNWKQAEEERIQKEKASLTKTGLQCRLCGNDHMTMNCPFN",
    "response#3": "DGSRSIITYKIEDGVKYKITQKVKEVKVLEKVHKSVAERKNWHKYGSEKGSPAGPSAVTARLGEEVELRLSRNWKQAEEERIQKEKASLTKTGLQCRLCGNDHMTMNCPFS"
  },
  {
    "reference": "MPNTAKKHREALAKIDRSRTYPLVEGIESVKSAAYAKFDETVEVAVRLGVDPRHADQMVRGAVVLPNGLGKDVRVLVFAKGEKEKEAREAGADYVGAEDLVTKIQEGWFEFDTAIATPDMMGVVGKIGKLLGPRGLMPNPKVGTVTFDVGRAVKESKAGKVEFRVEKAGIVHAPVGKVSFDADKLKENLLALVEALLKAKPSAAKGTYVKKISISSTMGPGLNLDISDVQAKLV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design must be focused on enhancing the efficiency of translation. The designed protein sequence is ",
    "response#1": "DETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAA",
    "response#2": "DETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAC",
    "response#3": "YDCYANLALLKLYQFNPHLSRDETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAKGTVVREC"
  },
  {
    "reference": "MEPSSPRKLGRMQEDGFELQPRRTTDRGLVSNLRRSTSSLFKSRRLQSTFGNSEKQENLSSWIPENIMKKECVYFVESSKMSDAGKVVCMCGYTREQHLEEAIKAHVFQGQEWDPRKHIQEMPTDAFGDIVFIGLGQKEGKYVRVSQDTPSSVIYHLMTRHWGLDVPNLLISVTGGAKNFNMKPRLKSTFRRGLVKVAQTTGAWIITGGSHTGVMKQVGEAVRNFSLSSSYKEGDVITIGVATWGTVHNRESLVHPKGGFPAEYVLDEEGQGNLTCLDSNHSHFILVDDGTHGRYGVEIPLRTRLEKFISEQTKERGGVAIKIPIACMVLEGGPGTLQTIYNAITSGIPCVIMAGSGRVADVIAQVANLPISEITVSLIQKKLSMFFQEMDIFREIRIVEWTKKIQDIVRRRQLLTVFREGKDGQQDMDVAILQALLKASRSHDHFGHENWDHQLKLAVAWNRVDIARSEIFTDERQWKPSDLHPMMAAALISNKPDFVKLFLENGVHLKEFVTQDTLLYLYETKEGFCFFYSKRKSECIPDPSLPCPSTSPRSLGSHALVQMMSLLSLAARVHSEIPRKKRLVLMSAVHQVQGVSLRSPCKQSPGHVTSTMDPVRDLLIWAIIQNRQELAEIIWAQSQDCTMAALACSKLLKELSKEEEDTDSSEEMLALAEEYELKAIGVFTECYRKDEDRAQKLLIRVSEAWGKTTCLKLALEAKHMTFVSHGGVQAFLTKVWWGQLCVDNGLWRVTLCMLVFPLVFTGLISFRE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The designed protein should be involved in calcium-mediated signaling using intracellular calcium source, regulation of actin cytoskeleton reorganization by localizing to lysosomal membrane, plasma membrane, cytoplasmic vesicle membrane and exhibiting manganese ion transmembrane transporter activity, mono-ADP-D-ribose binding. The designed protein sequence is ",
    "response#1": "PDLNTGIAKVVVEEGSRFAEEEQKVTAYVEVYLNAKLVLTTGKATDTGTLKWNSDYEAVIADRRKTRYKFVVKDGKGEEIGSTIQTLNDLIDRSQVNKNLIPLKNQKGDIKITTYWRPS",
    "response#2": "EKKLPDGSVEELPDLNTGIAKVVVEEGSRFAEEEQKVTAYVEVYLNAKLVLTTGKATDTGTLKWNSDYEAVIADRRKTRYKFVVKDGKGEEIGSTIQTLNDLIDRSQVNKNLIPLKNQKGDIKIL",
    "response#3": "PSVHKYNVSGANGTCLLASMGLQLNVTYSKRDNTTVSGLFSIDPSNTSATGSCGAQLVTLDLRSSRIHSLLFQFGMDPNTSQFFLQGIQLNMTVPDARDPTFQADNSSLRALQATIGNSYKCNAEERVEVTEAFSVNIFKVWVQAFQVC"
  },
  {
    "reference": "MSLVHLLLFAGLVIAASSSSPGSRLILREISDQKDKAVELNTTNFDSVLKDTPAKYAVVEFFAHWCPACRNYKPHYEKVARLFNGPDAIHPGIVLMTRVDCAMKTNTKLCDKFSVSHYPMLFWGPPTKFVSGSWEPKKDKSEILVIDDGRTAERLLNWINKQIGSSYGLDDQKFKNEHALSNLTDYNQISQAVYDVEEATAEAFDIILAHKAIKSSETSASFIRFIQLLAAHHLSRRCRKGAAEILVNYDDLCPSGNCSYEKSGGNDTLGNFPICGKDVPRGYYMFCRGSKNDTRGFSCGLWVLMHSLSVRIEDGESHFAFTTICDFVNNFFMCDECRLHFNDMCLSVKTPFKKARDFVLWVWSTHNKVNERLLKDEASLGTGDPKFPKIIWPPKELCPLCYLSSNQKSIEWDHEHVYKFLKNYYGPKLVSLYKEKSVSRSKEETVSATEDLTVATNALVVPIGAALAIAIASCAFGALACYWRTQQKNRKPRRR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide; May contribute to disulfide bond formation in a variety of secreted proteins .2. The protein can be targeted to Golgi membrane, extracellular space and perform flavin-linked sulfhydryl oxidase activity.3. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile to enable effective catalysis of O2 + 2 R'C(R)SH = H2O2 + R'C(R)S-S(R)CR'. The designed protein sequence is ",
    "response#1": "CSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQWCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQWSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQ",
    "response#2": "SESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQWSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQ",
    "response#3": "CSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQWSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQWSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQSESHFRGFPCSLWILFHFLTVQASQKNAESSQKPANGQEVLQAIRNYVRFFFGCRDCANHFEQMAAGSMHRVKSPNDAVLWLWTSHNRVNARLAGAPSEDPQFPKVQ"
  },
  {
    "reference": "MEIKLDTIEEAIEEIKAGKAVIVVDDEDRENEGDFVTAARNATPEMINFMALHGRGLICVPMSGERCDELKLDPMVGKNTSNHETNFTVSVDLLGYGCTTGISASDRSKTVKALINPEIKPEELGRPGHIFPLRAMEGGVLRRSGHTEATVDLSRLAGFEPAGVLVEIMNEDGEMARLPDLYKIARRFNLKLICIKDLIAYRLKHDSLIRKEVSVKMPTAWGEFNLTAFTQTDTGEPHLALTKGSWEKDEPVLVRVHSSCVTGDIFGSCRCDCGPQLHKAMEMIEKEGKGVILYMHQEGRGIGLVNKLKAYNLQEHGLDTVDANLELGFKMDQRDYGVGAQILRYLGVSKMRLMTNNPTKRAGLIGYGLEVVENVPIEIKSNLHNELYLRTKRDKMGHMIMQDVGHIK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis pathway to avoid interference with native metabolic processes.2. The presence of GTP cyclohydrolase II domains is critical for the interaction of the designed protein with its intended targets.3. The Nucleophile; for GTP cyclohydrolase activity active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.4. For general function, the protein need meet that Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.5. The designed protein should have a binding site that is specific to the target D-ribulose 5-phosphate, GTP ligand.6. The designed protein must be optimized for GTP cyclohydrolase II activity, manganese ion binding, zinc ion binding, GTP binding. The designed protein sequence is ",
    "response#1": "ERVAEADMPTKYGDFRMIGFINRLNGEHHVALVKGDVATEEPVLVRVHSECLTGDAFGSLRCDCGEQYAEAMKRIEAEGRGVLLYMRQEGRGIGLINKIKAYALQDQGYDTVQANRMLGFEDDLRDYGIGAQILMELGLHKIRLMTNNPQKITGLSGYGLEIVERERVAEADMPTKYGDFRMIGFINRLNGEHHVALVKGDVATEEPVLVRVHSECLTGDAFGSLRCDCGEQYAEAMKRIEAEGRGVLLYMRQEGRGIGLINKIKAYALQDQGYDTVQANRMLGFEDDLRDYGIGAQILMELGLHKIRLMTNNPQKITGLSGYGLEIVERTDVNFPTKHGKFILYLYQSSLDKKKQVALVKGTIKPNEPVLVRMHSECLTGDVFHSLRCDCHDQLNAALDMIEQEGTGVLVYMRQEGRGIGLTNKILAYKLQEEGKDTVEANEFLGFKADVRDYGIGAQILLDLGVRKIRLLTNNPKKVVGLQGYGLEIVERVLE",
    "response#2": "LATTKISTKYGGEFTVIPFSNIVDDAVHIAFVKGDIKTDDEVLVRVHSQCLTGDVFGSKRCDCGEQLHKAMKMIAKEGKGVLLYMRQEGRGIGLVNKLKAYELQDSGFDTVEANVKLGFKPDLRDYGIGAQILVNLGVRKMRLMTNNPRKIVGLEGYGLSVLKRIGGLKGIENRKVVSLPTEFGDFSLYLYRAMMDGQHHVALVKGDVAGQSNVLVRVHSECLTGDVFSSQRCDCGDQLRRAMQMIAKEGRGVIVYLRQEGRGIGLSHKIHAYELQEKEGLDTVEANERLGFKADLREYGVGAQILVDLGLHTIRILTNNPKKVVGLDGFGLKIAKQLRVAETKLPTQYGEFKAVAYASIIDSDEHVVLVKGDISTNDPVLVRVHSQCLTGDTFGSQRCDCGEQIHRSLEAIQAEGRGVFLYMRQEGRGIGLHNKLRAYQLQDAGLDTVEANEKLGFPADLRWYGIGAQILVDLGIKRIRLLTNNPKKVVGLDGYGLEIMERVRTAEAVLPTKYGQFQLVAYESVTDXKTHIALKQGQIVADRPTLVRVHSQCLTGDVFGSLRCDCGEQLDRALELIAEEGEGIVLYMRQEGRGMGLGNKIRAYQLQDQEGLDTVEANEKLGFPADLRDYGIGAQILRDLGXGKMRLLTNNPRKISSLEGHALEVVERVG",
    "response#3": "LATTKISTKYGGEFTVIPFSNIVDDAVHIAFVKGDIKTDDEVLVRVHSQCLTGDVFGSKRCDCGEQLHKAMKMIAKEGKGVLLYMRQEGRGIGLVNKLKAYELQDSGFDTVEANVKLGFKPDLRDYGIGAQILVNLGVRKMRLMTNNPRKIVGLEGYGLSVLKRIAGKEGPSYHDLATTKISTKYGGEFTVIPFSNIVDDAVHIAFVKGDIKTDDEVLVRVHSQCLTGDVFGSKRCDCGEQLHKAMKMIAKEGKGVLLYMRQEGRGIGLVNKLKAYELQDSGFDTVEANVKLGFKPDLRDYGIGAQILVNLGVRKMRLMTNNPRKIVGLEGYGLSVLKRIAGKEGPSYHDQAEVKIATKFGNFDFAGYSDKIENKEYIAIIKGDIRNKENVSVRLHSECLTGDVFGSKRCDCQDQLHRALREIEEKGEGLLIYSRQEGRGIGILNKLKAYRLQDEGYDTVEANHQLGFEDDLRDYAIAAQIIKDLGVKSVSLKTNNPLKIKGLEQYGVTVASTDVNFPTKHGKFILYLYQSSLDKKKQVALVKGTIKPNEPVLVRMHSECLTGDVFHSLRCDCHDQLNAALDMIEQEGTGVLVYMRQEGRGIGLTNKILAYKLQEEGKDTVEANEFLGFKADVRDYGIGAQILLDLGVRKIRLLTNNPKKVVGLQGYGLEIVERVAGKKGPSYHAA"
  },
  {
    "reference": "MSDKKRVLVKFSGEALAGGNGFGIDTHILKYIAGEIKGLVLNGIEIGIVIGGGNIIRGVSAAKDGIIKRTSGDHMGMLATVINAIAMREALEYSGLSVRVQSAIKMEAICETFIMGRAQRHLEKGRVVIFAAGTGNPFFTTDTAATLRAIEIDANMIIKATKVNGVYDRDPMKFEDAVLLNEISYERAMEDNIKVMDDTAIALAKDNKLPIVVCNMFEEGNLLKIANGDYTNCSVVKN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The UMP, ATP binding site should be stable and able to withstand changes in environmental conditions.2. The protein should have a low Km value for ATP + UMP = ADP + UDP.3. The designed protein should have ATP binding, UMP kinase activity within cytoplasm to regulate 'de novo' CTP biosynthetic process, phosphorylation.4. The protein must exhibit the following characteristics: Catalyzes the reversible phosphorylation of UMP to UDP..5. Ideal protein should have a binding affinity for key enzymes in the CTP biosynthesis via de novo pathway, UDP from UMP (UMPK route): step 1/1 pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MGYRVLLKVSGEAFVGEKRFGFDPAVVLRLSRDLKNVKESGVELCIVVGGGNIFRGASTSDGFERTSNDYVGMLATVINALALQNALEEMGVECRVLSAMPMTAVCETYVRRRAVRHLEKGRVVICAAGIGSPFFTTDTAAALRGIEMRCDAIFKGTQVDGVYSSDPKKDGSAVRYDRISYHDLLSSNLKIMDAAAISLARENSVPIIVFDIIEEENLYKALSGKRILLKLSGEALMGPSPFGIDPETVASMAAEVKEAKDRGLEICLVIGGGNIFRGMAGAAKGMDRAQADYMGMLATVMNALAMQNALEQLGVQTRVQSAIEMDKVCEPVIRRRAERHMEKGRVVIFAAGVGAPYFTTDSGAALRAAEMKCDALLKGTSVDGVYNADPKQDANAVRYDRLSYDRVLADNLKVMDASAVALCRDNHIPIVVFSIRKGNAVKKACIGS",
    "response#2": "MVIKRVLVKFSGEALAGKEGYGIDTKILKFIASEIKALVDAGMEVAIVVGGGNIIRGVSAAADGIIKRTSGDYMGMLATVINGVAIQEALEHIGLEARLQSAIDMHEIGEAFIIRRARRHLEKGRVVIFAGGTGNPYFTTDTAATLRASEIEAELLIKATKVDGVYDKDPNKFDDAVKLDTLTYEQALTKDIKVMDDTSIALARENSLPIVVCSAFRSGNIERALAEGEVRSLV",
    "response#3": "MGYRVLLKLSGEAFGGGQLGVNPDIVSGIAREIAQAATDVEIAIVVGGGNFFRGAELSQRGMDRGRADYMGMLGTVMNSLALQDFLEQAGAETRVQSAIEMIQVAEPYIPLRAERHLEKGRIVIFGAGAGLPYFSTDTVAAQRALEIIADVVLVAKNGVDGMYDDDPRTNPDARKIDQISHQEALKQNLKAVDSTALSLCMDNGMPMRIAPLADTNAERLAQGKRILLKLSGEALMGDDAFGINRATIVRMVEEIAEVTRMGVQVAVVIGGGNIFRGVAGGSVGMDRATADYMGMLATVMNALALGDTMDKAGLVARVMSAIGIERVVEPYVRPKALQYLEEGKVVVFAAGTGNPFFTTDTAAALRGAEIGAEIVLKATKVDGVYSADPKKDPEATRYTSITFDEVIGKNLEVMDATAFALCRDQKLPIKIFRYDGETGLAEVLMGRDLGTMIGH"
  },
  {
    "reference": "MAKIIGIDLGTTNSCVAVMEGSTPKVIENAEGARTTPSIVAFTEDGERLVGQPAKRQSVTNPERTFFAVKRLIGRRYDDPTVEKDKHLVPYSIVRADNGDAWVEADGKKYSPSQISAFVLQKMKETAESFLGEKVEKAVITVPAYFNDAQRQATKDAGRIAGLEVLRIINEPTAAALAYGLDKKSAGTIAVYDLGGGTFDVSVLEIGDGVFEVKSTNGDTFLGGEDFDMRLVTYLADEFKKEQGIDLRNDKLALQRLKEAAEKAKIELSSATQTEINLPFITADATGPKHLTLKLTRAKFEALVDDLIQRTVEPCRLALKDAGLTAGQIDEVVLVGGMTRMPKVQEVVKQFFGKEPHKGVNPDEVVAIGAAVQAGVLQGDVKDVLLLDVTPLSLGIETLGGVFTRLIDRNTTIPTKKSQTFSTAEDGQTAVTIRVFQGEREMAADNKMLGQFDLMGIPPAPRGVPQVEVTFDIDANGIVQVSAKDKGTGKEQQIRIQASGGLNDADIEKMVKDAEAHAAEDKKRRALVEAKNHAEALVHSTEKAVAEHGDKVGAVEKGAIEAALADLKSTLEGDDVEAITAKTNTLAQASLKLGEAMYAAQQPGAEGAAKPADDVVDAEFTEVDDDKKKSA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be optimized for ATP-dependent protein folding chaperone, unfolded protein binding.2. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "MFL",
    "response#2": "MAPKLTI",
    "response#3": "MELV"
  },
  {
    "reference": "MTTAHSAPRDNSDKPVIWTVSVTRLFELFRDISLEFDHLATITPIQLGFEKAVTYIRKKLATERCDAIIAAGSNGAYLKSRLSIPVILIKPSGFDVLQALAKAGKLTSSIGIVTYQETIPALLAFQKTFHLRLEQRSYVTEEDARGQINELKANGIEAVVGAGLITDLAEEAGMTAIFIYSAATVRQAFHDALDMTRLTRRQRVDYPSGKGLQTRYELGDIRGQSPQMEQLRQTITLYARSRAAVLIQGETGTGKELAAQAIHQTFFHRQPHRQNKPSPPFVAVNCGAITESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLPLQTRLLRVLEEKAVTRVGGHQPIPVDVRVISATHCDLDREIMQGRFRPDLFYRLSILRLTLPPLRERQADILPLAESFLKQSLAAMEIPFTESIRHGLTQCQPLLLAWRWPGNIRELRNMMERLALFLSVDPAPTLDRQFMRQLLPELMVNTAELTPSTVDANALQDVLARFKGDKTAAARYLGISRTTLWRRLKAGAKDQSDN",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The H-T-H motif domain within the designed protein should exhibit high affinity and specificity towards its target DNA sequence. The designed protein sequence is ",
    "response#1": "NNFWETSGLNILETLARLDHDSVPQLIDNVLAVRTNIAAIFIRAAVRQHPDRVQAIVADAPTVEDSADAFADLDYRIFRALAFACGNPIYGLILNGLKGLYTRVGRYYFSNPQARRLALAFYSRLGELAASRQYDRVMDFVRSYGKESGAIWHGMQSGIPRD",
    "response#2": "NNFWETSGLNILETLARLDHDSVPQLIDNVLAVRTNIAAIFIRAAVRQHPDRVQAIVADAPTVEDSADAFADLDYRIFRALAFACGNPIYGLILNGLKGLYTRVGRYYFSNPQARRLALAFYSRLGELAASRQYDRVMDFVRSYGKESGAIWHGMQSGIPRDD",
    "response#3": "NNFWETSGLNILETLARLDHDSVPQLIDNVLAVRTNIAAIFIRAAVRQHPDRVQAIVADAPTVEDSADAFADLDYRIFRALAFACGNPIYGLILNGLKGLYTRVGRYYFSNPQARRLALAFYSRLGELAASRQYDRVMDFVRSYGKESGAIWHGMQSGIPRDD"
  },
  {
    "reference": "MAAMTTPRLVLASHNEHKLGELRAILRPVVGDLDPAQVVSAREFGAPEPIEDGLTFAENALIKARALAAATGLPSVADDSGLAVDVLGGAPGIFSARWSGRHGDDAANLDLLLAQISDVALEHRLAQFVCAAALVTPQGDEHVELGRMHGYLLTERRGDGGFGYDPIFVPEGATRTTAELSPEEKNAISHRGRAFRALAPHIAWALAVG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must be optimized for dITP diphosphatase activity. The designed protein sequence is ",
    "response#1": "GMVGH",
    "response#2": "MDKKS",
    "response#3": "MS"
  },
  {
    "reference": "MPNQKTLQRGFTLTGKGLHTGLDIELHFLPAPENHGFIIKRIDLEGQPTIPALAEYVTQTTRGTVLSHNNVQISTIEHAMAALFASEIDNCLMEVNAAEFPILDGSAKPFIQKIREVGIVEQEAEKEYFIVKKKIEYTDPNTHSSITILPDDHFSIQTLIGFNSSILHNQYAVLDDLSLFATEIASCRTFVFVRELEELLKMDLIKGGDLENALVIYDEIIDQKELDRIADLLNHPHKKVDELGYLSELQFDNEPARHKLLDVLGDLALIGKPIKGRVIATRPGHKTNTSVSKLIRKEIKRQEVPLPDYDPNGEPFLDINQIKKMLPHRWPFLMVDKVLNINQTVITGLKNVTVNETFFNGHFPKEPVMPGVLLVEAMAQTGGLLILHQVENPELYSTYFMKIDAVKFRQKVVPGDTVIFRLELLSPIRRGCATMKGYAFVGEKIVCEAEFMAQITKNK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that can perform metal ion binding, (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity, UDP-3-O-acyl-N-acetylglucosamine deacetylase activity, UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity, 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity in cytoplasm to support fatty acid biosynthetic process, lipid A biosynthetic process.2. The protein requires Zn(2+) to activate a critical catalytic site.3. The protein's active site must be carefully engineered to ensure that the amino acids involved in a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine +   H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate are properly positioned for optimal catalysis.4. For general function, the protein need meet that Involved in unsaturated fatty acids biosynthesis; Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.5. The protein should have a high affinity for Zn(2+). The designed protein sequence is ",
    "response#1": "MHGSPPRSSTRNRSRRRTPTARPAGRPSPRSRRGSTPRCAPARPVRTPRRSPRPRGRPGRGGRPARSGSRAPSRGTGPRRPPPRRRRPRTRWPRPRRRPRRRRRRRRGRRGRRSPRRSAPRPRGPPRPRRCRPPRRRERRLAPRPSPPCRPDRARASRPGATSLRRPPRPSSRRPRRSRCHAGPARPPSRAPRRRAHPARRAPRRRSRSRPAPGRSPARRRGARSRSCRASSRPRRSPAAERLPPRTNCPGPAPRRPSPRRRPRPASRRRRPPPSPASSSPTSSRSPTRAARPRARSRRRARSPRRASPPARRSSNRSRPHRRRRRPGRSGARRLARRRRPACRGGRPARRRPGRAPARPAGRAPARPAARAPARRRRRRARRPARRRRPGRRRRLRRQRRRPARGRSRCGARRARPCRRGSRARPRRRARIDRRRRRGRRSRGRCPRRRPGRRRPSRRRRPRPARRRRRGRRASRRSARRRRGGRRRSSRRRRSARRRRRHRARRRRARRGRRRRRGRRRRRARRRGGGRRGRT",
    "response#2": "MKSAXLKDILYKVTINAVTGSTDIAXNXVXDSVTYXSLAFLENPEFLSSLDNSEAGAIIAXKDLSDXLPSXIIKTKNTRLAMSAIAAHFFXNPSNKLXVIAXHHPSVTIHPSXEVGPGSIIXPQVTIGXNVTIXAXVXIXXNXIGDXNXIHXGVXXGAXFGXDXXGXYXIIXXGGVXIGHDXIXXKNXKIGANTTLDKATLGNIVFHGXGXVGSSTXICAXVGXNXIAXYXXIAXVGIYAKXAXSXVXMXIAXVXIXVXKHXXKXNXXXSXIKKXIWXXXIYXLXRKXXKX",
    "response#3": "MSKNYSLETLAKGIGAELIGNEDVEITDITHDSRRAGPGALFIARGGSAVDGHDFIADALAKGATTVISERKVDVPEGVPLILVPDTRTALADISAYFFGHPTDALKVLA"
  },
  {
    "reference": "MGPVLGAEGCREAPARRPAPRGLGRAGPMHSLAAGPDCCGGLGNLDFRQADLCVMTRLLGYVDPSDPRFVAAVLAIAFNPLFWNVVARWEHKTRKLSRAFRSPHLACYTLGGAILLLNVLRSHCFMQAMLSQPRMQSLDNPTAYRAGLALLGVGSVFVLSSFLALGFTGTFLGDYFGIFKEARVTSFPFNILDNPMYWGSTANYLGWAIMHASPTGLLLTAGGGPHLRCRHPVRGALHR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be able to perform phosphatidyl-N-methylethanolamine N-methyltransferase activity under specific conditions.2. For general function, the protein need meet that Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure The designed protein sequence is ",
    "response#1": "MSSN",
    "response#2": "MK",
    "response#3": "MTMITDKLRHAS"
  },
  {
    "reference": "MSFDTIEEALSALKKGEIIIVVDDEDRENEGDLVVLAEKATPEAINFMITHGRGLVCVPMTEERARELDLPLMVHHNTDRHGTAFTVSVDAMETSTGISAFERSLTVMQLIDPNRGAQDFRRPGHIFPLIAREGGVLRRAGHTEAAVDLARLCGAYPAGVICEVIKEDGTMARVPDLKEMSRKFGLKMITIQDLIRYRRQKETLIERVVSVRMPTEYGEFQMIGYTNEIDEKEHVAFVKGEIDPEQPVLVRVHSECLTGDVFGSKRCDCGPQLHAALRKIEEAGQGVLLYMRQEGRGIGLINKLKAYKLQEEGLDTVEANLRLGFRDDLREYGIGAQILRDLGVGKMRLMTNNPRKITGLKGYGLEVTEVVPLEIPANKNNRRYMETKKEKLGHMLHL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must be optimized for GTP cyclohydrolase II activity.2. The designed protein should contain one or more GTP cyclohydrolase II domains that are essential for its function. The designed protein sequence is ",
    "response#1": "KEVTVAMPTKLGEFKLTAYTQNTTGEVHLAMVMGEWEENEPVLVRVHSSCATGDILHSLRCDCGDQLAKAMEMVEAEGKGIVLYMKQEGRGIGLINKLKAYKLQEEGKDTVEANLALGFDMDNRDYGIGAQILRDLGVSKIKLLTNNPKKRVGLIGYGLEIVDNR",
    "response#2": "RVAEARLPTPFGEWRVVGYRNDVDRAEHVALVYGDVTLKPGTLVRMHSKCLTGDVFGSSRCDCGWQLHAAMQMIAQDGRGVVVYLDQEGRGIGLLNKLKAYELQDAGHDTVEANEKLGFGPDLRNYGIGAQILLDLGLQSIRILTNNPMKLVGVDGYGLTILERVG",
    "response#3": "GVDMPTEWGHFDLIAYKQSNTGDTHLALVKGTWEPNEPVLVRVHSSCITGDIFGSCRCDCGEQLHAAMKRIEAEGKGVVVYMFQEGRGIGLLNKLKAYKLQEMGRDTVEANLDLGLPMDSRDYGIGAQIVRDLGIRKLRLLSNNPKKRAGLMGYGLEIIDTVL"
  },
  {
    "reference": "MNXLNSKNQSSGDTDSPLAAVEDAIAAIGRGEIVVVVDDEDRENEGDLIMAAEHATPXTIAFFVRYTSGVLCAPMTGERLDELALPLMVTINTEPMRTAYTITVDAAEGTTTGISAGDRSRTLSLLSSPYSEPHDFVRPGHVLPLRYRPGGVLKRAGHTEAAVDLSRLAGCEPTGVLAEVVNDDGTMARLPQLIQFCEEHDLFLISIADLIRYRRANXRLVDPVAQARXPTIYGDFTGHVXRDFDGTEHLALVXGQIMGSDPXLVRVHSECLTGDILTSMRCDCGGQLHTALEAIAKEQAGVLVYLRGHEGRGIGIGHKIAAYSLQDEGRDTVDANLELGLPIDSREYGIGAQILVELGVTKMRLMTNNPDKIGGLGGYGLEIVERVPTTTGANRENLAYLRTKQMRLGHLIEGLDDVAAD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should consider the endogenous proteins and enzymes present in the riboflavin biosynthesis pathway to avoid interference with native metabolic processes.2. The designed protein should have 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, magnesium ion binding to facilitate riboflavin biosynthetic process.3. The designed protein should have a high specificity for GTP binding, with minimal off-target effects.4. The Nucleophile; for GTP cyclohydrolase activity, Proton acceptor; for GTP cyclohydrolase activity active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "RMSMARLPTRYGVFTVFGYREISSGQEHAALVMGDLASCKSPLVRVHSECLTGEVFGSIRCDCRAQLDLAMERIAQAGCGIVVYLKGHEGRGIGLTEKLRAYALQDKGLDTVEANLQLGLQADARDYAVGAQILQDQGVTTMKLMTNNPRKYRGISEYGLSIEDRVG",
    "response#2": "RMSMARLPTRYGVFTVFGYREISSGQEHAALVMGDLASCKSPLVRVHSECLTGEVFGSIRCDCRAQLDLAMERIAQAGCGIVVYLKGHEGRGIGLTEKLRAYALQDKGLDTVEANLQLGLQADARDYAVGAQILQDQGVTTMKLMTNNPRKYRGISEYGLSIEDRVAA",
    "response#3": "RVASAMLPTEHGPFRIHTFENQIDNQTHVALVCGEIGDGKDVLVRVHSCCLTGDVFHSIRCDCGAQLDAAMRRLAAEGRGVLLYLNQEGRGIGLANKIRAYALQDDGFDTVEANERLGFKADQRDYGVGVQILRELGIRSMRLLSNNPRKLVGIEGYGLSVSEWR"
  },
  {
    "reference": "MALRIFVTGPAGVGKTTLVERVAREVDRWGYIVGGVITREVRRGGRRIGFKITALDTGEEGTLASLRGTSHLPGVPFGKYVVHVDEIERVAVPAIRRAIVEADLIVIDEIGPMEYTSNEFIRAVGEVLKSEKPLLAVVHRKFIDRFRPLGEVHTLSFENRNAEFGIILDRVMKELKGIRG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must have high specificity towards ATP binding. The designed protein sequence is ",
    "response#1": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#2": "LEEFDQIINSTPGFVKGMWCGERECEDLVKEKTGATARCMPLEQEQLSDKCMCCGKPAKSMVYWGKAK",
    "response#3": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYV"
  },
  {
    "reference": "MGGKWSKRSIVGWPQIRERMRRTPPAAEGAAEGVGAASQDLDKHGAITSSNTASTNADVAWLEAQEDEEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLDGLIYSKKRQEILDLWVYNTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPMDPADVEKANEGENNCLLHPICHHGMDDEDGEVLVWRFDSKLALTHKARELHPEYYKDC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis.2. The ideal protein would be one that plays a critical role in suppression by virus of host autophagy through GTP binding within extracellular region, membrane.3. The designed protein should contain at least one Diacidic; necessary for CD4 internalization motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "GFSSIF",
    "response#2": "EAEETR",
    "response#3": "GYEE"
  },
  {
    "reference": "MSNEMKCPVPHGQHPSAATTATETSSPMHGAVTHNSRKIVRNAEWWPDRLDLAVLHQNTKLADPMGDGFDYAEEFKKLDLDALIKDLNALMTDSQPWWPADFGHYGPFFIRMAWHSAGTYRIYDGRGGAGMGTQRFAPLNSWPDNVSLDKARRLLWPIKQKYGKSISWADLMILTGNVALESMGFKTFGFAGGRADVWVPQEDIFWGAEHTWLGSDRYQGERDLDNPLAAVQMGLIYVNPEGPDGKPDPIASARDIRETFGRMAMNDEETFALIAGGHTFGKGHGAHDPGPNVGPEPEGAPIEQQGLGWKNSVGKGHSEDTISSGLEGAWTSQPVKWDSEYLDNLYNFDWALKKGPGGAWQWYAVAETPNIPDAFIEGKKHLPMMFTSDLALKFDPAYEKIGKRFQADPALFADAFARAWFKLTHRDMGPIVRYLGPLVPKEVLIWQDPVPAVDHELIGESEIAELKAKILASGLTTSQLISTAWASASTFRGSDKRGGANGARIRLEPQKSWEVNQPQVLAKVLSTLEGIQQGFGKKVSIADLIVLGGSAAVEEAAKKAGYEVKVPFTPGRTDASQEFTDVHSFAPLEPTADGFRNYLRLGHTQRAEELLVDRAQLLKLTAPEMTVLVGGLRVLGANFRGGKDGVFTDKPGTLTNDFFVNLLDMATEWKASTTAEGSFDGLDRETGELKYTATRVDLIFGSNSQLRALAEVYAASDAKETFVKDFVAAWVKVMNLDRFDLA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The presence of heme b is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "VSMADLIVLAGCAGVEQAAKAGGHQIEVPFTSGRGDASAEQTDAESFAVMEPVMDGFRNYQARELSTSPEEMLVDRAQLLGLSAPEMTVLVAGLRVLGANHGGSAHGVLTNRPGVLSSDFLTNLLDMGTEWKPSGKGVYEGRDRASGAARWTATRVDLVFGSNSQLRALAERYAQDDAEASFVADFVAAWVKR",
    "response#2": "VSMADLIVLAGCAGVEQAAKAGGHQIEVPFTSGRGDASAEQTDAESFAVMEPVMDGFRNYQARELSTSPEEMLVDRAQLLGLSAPEMTVLVAGLRVLGANHGGSAHGVLTNRPGVLSSDFLTNLLDMGTEWKPSGKGVYEGRDRASGAARWTATRVDLVFGSNSQLRALAERYAQDDAEASFVADFVAAWVKA",
    "response#3": "VSMADLIVLAGCAGVEQAAKAGGHQIEVPFTSGRGDASAEQTDAESFAVMEPVMDGFRNYQARELSTSPEEMLVDRAQLLGLSAPEMTVLVAGLRVLGANHGGSAHGVLTNRPGVLSSDFLTNLLDMGTEWKPSGKGVYEGRDRASGAARWTATRVDLVFGSNSQLRALAERYAQDDAEASFVADFVAAWVKL"
  },
  {
    "reference": "DECCAIPLCAKIFPGRCP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that localizes to extracellular region and can perform toxin activity with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "QSPWFDPVKRCCSRYCWKCIPCCPX",
    "response#2": "MPQLDTSTWFSYV",
    "response#3": "MNILQNVF"
  },
  {
    "reference": "MPLLRKYEIMILLTEEFNDSELKTWVFNYAKNLRKFSVCDISVISRGKHNLAYPIETKMKGNYIQLNFSSMPKYITNFSKTLKMDSNVLRFLVFNKQL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein localizes to the ribosome. The designed protein sequence is ",
    "response#1": "ME",
    "response#2": "MRAHTDVVTAIATPIDNSDMIVTASRDRSIILWHLTKEDKTYF",
    "response#3": "MRAHTDVVTAIATPIDNSDMIVTASRDRSIILWHLTKEDKTYH"
  },
  {
    "reference": "MRVKGIRKNYQHLWRWGTMLLGMLMICNAAEQLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVELGNVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDLNNTTTDLNNTANTHNSSEGKMEKGEIKNCSFNITTSIRDKVQKEYALFYKLDVVPIDDDNTRYRLISCNTSVITQACPKVSFDPIPIHYCAPAGFAILKCNDKKFKGTGPCKKVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSENFTNNAKIIIVQLNKAVEINCTRPNNNTRKSINIGPGRAFYTTGEIIGDIRQAHCNLSRAEWNDTLKQIVIKLREQFGNKTIVFNQSSGGDPEIVMHSFNCGGEFFYCDSTQLFNSTWMANSTWENDNSTEGNIMLPCRIKQIINMWQKVGKAMYAPPIRGQIRCSTNITGLLLTRDGGDNSETTEIFRPGGGNMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVQREKRAVGLGALFLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNTSWSNKSLNQIWDNMTWMEWEREIDNYTGLIYTLIEKSQNQQEKNEQELLELDKWASLWNWFNISNWLWYIKIFIMIVGGLVGLRIVFSVLSIVNRVRQGYSPLSFQTRLPAPRGPDRPEGTEEEGGERDRDRSGQLVDGFLAIIWVDLRNLCLFSY",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be designed to localize specifically to plasma membrane, host cell endosome membrane in order to perform its role in clathrin-dependent endocytosis of virus by host cell, virion attachment to host cell, fusion of virus membrane with host plasma membrane more effectively.2. The protein must exhibit the following characteristics: The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.3. The protein should have Helical domains to ensure proper insertion into the membrane.4. The designed protein have Retroviral envelope protein GP41-like, Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains with specific sequence motifs that are crucial for its activity.5. The protein design should include a YXXL motif; contains endocytosis signal motif to ensure proper function.6. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLKNVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLSCTDVKGNDTAKNTTANTNSSGIVMETGEMKNCSFNITTEIRDQVKKERALFYRLDVVPIGNDTNSSKDNNSSSRERDYRLINCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNDNKTFNGSGTCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVMLRSENFTNNAKIIIVQLNESVVINCTRPNNNTRKSVHIGPGSAFYATGDIIGDIRQAYCTINRTEWNNTLKLIVAKLGKQFAKETIVFKPSSGGDPEIVTHSFNCGGEFFYCNTSQLFNSTWYNSTDGTWTGNGTKGNETITLPCRIKQIINMWQEVGKAMYAPPIKGQIRCLSNITGLLLTRDGGNQSSANETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTHAKRRVVQREKR",
    "response#2": "WVTVYYGVPVWKDAETTLFCASDAKAYEREKHNVWATHACVPTDPNPQEIPLENVTEDFNMWKNNMVEQMHTDIISLWDQSLKPCVTLTPLCVTLNCSTATPCTSNHECLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLKNVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLSCTDVKGNDTAKNTTANTNSSGIVMETGEMKNCSFNITTEIRDQVKKERALFYRLDVVPIGNDTNSSKDNNSSSRERDYRLINCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNDNKTFNGSGTCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVMLRSENFTNNAKIIIVQLNESVVINCTRPNNNTRKSVHIGPGSAFYATGDIIGDIRQAYCTINRTEWNNTLKLIVAKLGKQFAKETIVFKPSSGGDPEIVTHSFNCGGEFFYCNTSQLFNSTWYNSTDGTWTGNGTKGNETITLPCRIKQIINMWQEVGKAMYAPPIKGQIRCLSNITGLLLTRDGGNQSSANETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTHAKRRVVQREKRAALGAVFLGFLGVAGSTMGAASMTLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNKSQSEIWNNMTWMQWDREVINYTNMIYDLLEESQNQQEKNEQDLLALDKWASLWNWFDISHWLWYIKIFIMIVGSLIGLRIVFAVLSIINRVRQGYSPLSLQT",
    "response#3": "MGAASITLTAQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLQDQQLLGIWGCSGKLICTTAVPWNSSWSNKSYDEIWENMTWMQWDREISNHTNTIYQLLEVSQIQQEKNEKDLLALDKWQNLWNWFSISNWLWYIKMFIMIVGGLIGLRIIFAVISIVNRVRQGYSPLSFQTLN"
  },
  {
    "reference": "MLSKALFFSSLPLWAKVASAAFGITTTDSSYTIDAGSPNPLKFTVSRSSCDITSINYYGSELQYQSKGSHIGSGLGSASVSAVQNGDYIKVTCDTDTLTHYFVVHSGDPIIHMATYITAEPSIGELRFIARLNSNLLPNEEPFGDVSTTAGGSAIEGSDVFLVNGQTRSKFYSSQRFIDDQRHCISGSAHRVCMILNQYESSSGGPFHRDINSNNGGDYNSLYWYMNSGHVQTESYRMGLHGPYSMYFSRSGTPGTNIDTSFFANLDIKGYVPASGRGTVTGKASGADSNFKWVVHWYNDAAQYWTYTASDGSFTSPAMKPGTYTMAYYQGEYRVAETSVTVSAGSSTTKNISGSVKTGTTIFKIGDWDGQPTGFLNADKQLRMHPSDSRMSSWGPVTYTVGSSSVGSFPMALFKSVNSPVTIKFTATSAQTGAATLRIGTTLSFAGGRPQATINSYTGPTPSAPTNLNSRGVTRGAYRGLGEVYDVSIPAGTIVAGTNTITINVISGSSGDDFLSPNFVSAYPFLTLMRC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that contributes to polysaccharide catabolic process, cell wall organization by functioning in extracellular region with carbohydrate binding, rhamnogalacturonan endolyase activity.2. For general function, the protein need meet that Degrades the rhamnogalacturonan I (RG-I) backbone of pectin .; Pectinolytic enzymes consist of four classes of enzymes: pectin lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase3. The protein should be designed to have high stability and activity towards Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-   (1->4)-alpha-D-galactopyranosyluronic acid bonds of   rhamnogalacturonan I domains in ramified hairy regions of pectin   leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-   unsaturated D-galactopyranosyluronic acid at the non-reducing end..4. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "LEVPLAKPEGGVTPDGKPYTYSANDASVGDIDGDGEYEMILKWDPSNSKDNAHDGYTGEVLIDAYKLDGTFLWRINLGRNIRAGAHYTQFMVYDLDGDGKAEIAMKTADGTTDGKGHIIGDEQADFRNEQGRILSGPEYLTVFKGETGEALTTVEYEPPRGKLEDWGDGYGNRMDRFLAGTAYLDGERPSLVMARGYYTRTVLVAYDFRNGRLKKRWVFDSNQPGHEAYAGQGNHSLSVADVDGDGKDEIIYGAMAVDHDGTGLYSTGLGHGDAMHVGDLDPSRKGLEVFQVHEDATKPYGLSLRDAGTGEILWGVHAGTDVGRGMAAHIDPSYKGSLVWGIDPPGNDGMSYGLFTSKGEKISDKAPSSANFAIWWDGDLVRELLDHDWDGTIGRPKIEKWDAENGCLKTIFQPAGVLSNNGTKGNPVLQANLFGDWREEVIWRTEDSSALRIYTTTHLTRHCFYTLMHDPVYRLGIAWQNTAYNQPPHTSFYLGTGMKKPPKDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGKVLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVKWKGHKVITSSTEAQAYTPGRFIA",
    "response#2": "LEVPLAKPEGGVTPDGKPYTYSANDASVGDIDGDGEYEMILKWDPSNSKDNAHDGYTGEVLIDAYKLDGTFLWRINLGRNIRAGAHYTQFMVYDLDGDGKAEIAMKTADGTTDGKGHIIGDEQADFRNEQGRILSGPEYLTVFKGETGEALTTVEYEPPRGKLEDWGDGYGNRMDRFLAGTAYLDGERPSLVMARGYYTRTVLVAYDFRNGRLKKRWVFDSNQPGHEAYAGQGNHSLSVADVDGDGKDEIIYGAMAVDHDGTGLYSTGLGHGDAMHVGDLDPSRKGLEVFQVHEDATKPYGLSLRDAGTGEILWGVHAGTDVGRGMAAHIDPSYKGSLVWGIDPPGNDGMSYGLFTSKGEKISDKAPSSANFAIWWDGDLVRELLDHDWDGTIGRPKIEKWDAENGCLKTIFQPAGVLSNNGTKGNPVLQANLFGDWREEVIWRTEDSSALRIYTTTHLTRHCFYTLMHDPVYRLGIAWQNTAYNQPPHTSFYLGTGMKKPPKL",
    "response#3": "LEVPLAKPEGGVTPDGKPYTYSANDASVGDIDGDGEYEMILKWDPSNSKDNAHDGYTGEVLIDAYKLDGTFLWRINLGRNIRAGAHYTQFMVYDLDGDGKAEIAMKTADGTTDGKGHIIGDEQADFRNEQGRILSGPEYLTVFKGETGEALTTVEYEPPRGKLEDWGDGYGNRMDRFLAGTAYLDGERPSLVMARGYYTRTVLVAYDFRNGRLKKRWVFDSNQPGHEAYAGQGNHSLSVADVDGDGKDEIIYGAMAVDHDGTGLYSTGLGHGDAMHVGDLDPSRKGLEVFQVHEDATKPYGLSLRDAGTGEILWGVHAGTDVGRGMAAHIDPSYKGSLVWGIDPPGNDGMSYGLFTSKGEKISDKAPSSANFAIWWDGDLVRELLDHDWDGTIGRPKIEKWDAENGCLKTIFQPAGVLSNNGTKGNPVLQANLFGDWREEVIWRTEDSSALRIYTTTHLTRHCFYTLMHDPVYRLGIAWQNTAYNQPPHTSFYLGTGMKKPPKDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGKVLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVKWKGHKVITSSTEAQAYTPGRFIA"
  },
  {
    "reference": "MLKNDLFLRALKRQPCSRTPIWVMRQAGRYLPEYRAIREKTDFLTLCKTPELAAEVTIQPVELMGVDAAIIFSDILVVNEAMGMDVEIIETKGIKLSPPIRSQVDIDRLSVPDVHDKLGYVLDAIRLTKKELDNRVPLIGFSGAAWTLFTYAVEGGGSKNYAFAKKMMFRDPKMAHMLLSKISSVISDYVCAQVEAGADAIQIFDSWASALSEDDYREFALPYIKENVQALKTKYPDIPVIVFSKDCNTILEDIAATGCDAVGLGWGIDIAKARTILGDRVCLQGNMDPTVLYGTPEKIKAEAGKILKQFGQHTANSGHVFNLGHGILPDVDPANLKLLVEFVKEESAKYH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design should take into account the physiological conditions of the Porphyrin-containing compound metabolism, protoporphyrin-IX biosynthesis pathway.2. The substrate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "ISGLAVAHHLRSRGTDAVLLESSARLGGAVGTHALAGYLVEQGPNSFLDREPATRALAAALNLEGRIRAADPAAKRRYVYTRGRLRSVPASPPAFLASDILPLGARLRVAGELFSRRAPEGVDESLAAFGRRHLGHRATQVLLDAVQTGIYAGDVEQLSVAATFPMLVKMEREHRSLILGAIRAQKAQRQAALPAGTAPKLSGALSTFDGGLQVLIDALAASLGDAAHVGARVEGLAREDGGWRLIIEEHGRRAELSVAQVVLAAPAHATAKLLRPLDDALAALVAGIAYAPIAVVHLGFDAGTLPAPDGFGFLVPAEEQRRMLGAIHASTTFPFRAEGGRVLYSCMVGGARQPGLVEQDEDALAALAREELKALAGVTARPSFTRVFRWPLGIPQYNLGHLERVAAIDAALQRLPGLHLIGNAYKGVGLNDCIRNAAQLADALA",
    "response#2": "ISGLAVAHHLRSRGTDAVLLESSARLGGAVGTHALAGYLVEQGPNSFLDREPATRALAAALNLEGRIRAADPAAKRRYVYTRGRLRSVPASPPAFLASDILPLGARLRVAGELFSRRAPEGVDESLAAFGRRHLGHRATQVLLDAVQTGIYAGDVEQLSVAATFPMLVKMEREHRSLILGAIRAQKAQRQAALPAGTAPKLSGALSTFDGGLQVLIDALAASLGDAAHVGARVEGLAREDGGWRLIIEEHGRRAELSVAQVVLAAPAHATAKLLRPLDDALAALVAGIAYAPIAVVHLGFDAGTLPAPDGFGFLVPAEEQRRMLGAIHASTTFPFRAEGGRVLYSCMVGGARQPGLVEQDEDALAALAREELKALAGVTARPSFTRVFRWPLGIPQYNLGHLERVAAIDAALQRLPGLHLIGNAYKGVGLNDCIRNAAQLADAVRS",
    "response#3": "YCCLAERSLMRHLEGGCSVPIGVQSHYNESTNELSLKAIIVSPDGIHFVEDEYKGVVNNKDDADAIGIKVGDLL"
  },
  {
    "reference": "MDYLPLFCKLENRECLLVGGGEIAERKARLLLEAGAKLTVNAQTFNEQFMCWNAQKKLVLVQGEFSAELLNKKWLVIAATDSQETNQKVSIEAENRCIFCNVVDSPKDASFIMPSIIDRSPIMVAVSSEGHAPVLARLLREKLESILPQHLGQLAKYAGYLRNKVKTTYQTIAERRRFWEKLFAHDRLAQALANKDFEHVEKITNELFDTSLDYRGEVVLVGAGPGDAGLLTLKGLQQIQQADIIVYDRLVSDEIMDLARRDAQRIFVGKRPGFHCVPQEQINQILLEQAQKGKRVVRLKGGDPFIFGRGGEELETLFNADIPFSVVPGITAASGCSAYSGIPLTHRNYAHSVRLVTGHLKDGSHLDWQSLAAEKQTLVFYMGLSQAENIGKQLIKHGMDINTPVAIVEEGTTTKQKVISGVLSNLAQLSIQACSPSLIIIGPVVALREKLNWFSQH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Proton acceptor, Proton donor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The protein must exhibit the following characteristics: Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1.3. The designed protein should have precorrin-2 dehydrogenase activity, sirohydrochlorin ferrochelatase activity to facilitate cobalamin biosynthetic process, methylation.4. The designed protein should contain one or more Sirohaem synthase dimerisation domains that are essential for its function.5. The protein design should consider the endogenous proteins and enzymes present in the Porphyrin-containing compound metabolism pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "VGAGPGDPDLLTIKALNELQKADVVLFDQLVSPEILALIRPEAECISVGKKAGAHSMAQEDINQLLVVKAQEGHRVVRLKGGDPFMFGRGAEELQVLKAAGIAYQVVPGITAAAGATAYAGIPLTHRDHAQSAVFITGHAKRDGQDPDWESLAKQRQTLVIYMGLMRSAHIQQQLLKHGLASSTPVALIERGTTAEQRVVRGNLSELSQLAEKVVSPSLMVIGEVVASGASPAQARAAVGAGPGDPGLLTLNALRALNEADVILHDRLVSAEVLALARRDAELVDVGKQSGATLTSQEIIHSLLLEHARQGKRVVRLKGGDPFMFGRGGEELEFLRAHDIAYDVVPGITAASACAAYAGIPLTHREHAQSVRFLTAHGKDSLDALDWQALADDSQTLAVYMGLGLLETLQQRLLELGRSPNTAFALIENGSRPEQRIIRGTLDQLSELAREHQVQSPALLIIGKVVASGASPAQARAAA",
    "response#2": "VGAGPGDAGLLTLRGLQVIQQADVVLYDHLVSADILELVRRDAERICVGKRAGAHSVSQEETNRLLVTLAQQGKRVVRLKGGDPFIFGRGGEELQQAAQAGISFQVVPGITAAAGATAYAGIPLTHRDYAQSVVFITGHCRPDGQALEWATLARGRQTLAIYMGTVKAAEIAQQLIAHGRAAGTPVAVIGRGTRHDQQVLIGTLQQLEQLARQAPTPALLVIGEVVG",
    "response#3": "VGAGPGDPELLTLKALRLMQQADVVIYDRLVSEPILNLCRRDAIKVYVGKARSNHSVPQEGINALLVKYASEGKRVCRLKGGDPFIFGRGGEEIQELFAAGISFQVVPGITAASGCSAYAGIPLTHRDYAQSVRFLTGHLKEGSPELPWDELVYEHQTLVLYMGLMGLETICQKLIAHGQRPDMPVALISKGTTPEQKVVVGTLADIAAKVTEHQIHAPTLTIIGEVVGAGPGDAGLLTLKGLQQIQQADIVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHCVPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCQAGIPFSVVPGITAASGCSAYAGIPLTHRDYAQSVRLVTGHLKTGGELDWQNLAAEKQTLVFYMGLNQAAAIQQKLLEHGMPSDMPVAIVENGTAIKQRVVDGVLTGLAALATQMESPALIIVGRVVGAGPGDPGLLTLNALRALNEADVILHDRLVSAEVLALARRDAELVDVGKQSGATLTSQEIIHSLLLEHARQGKRVVRLKGGDPFMFGRGGEELEFLRAHDIAYDVVPGITAASACAAYAGIPLTHREHAQSVRFLTAHGKDSLDALDWQALADDSQTLAVYMGLGLLETLQQRLLELGRSPNTAFALIENGSRPEQRIIRGTLDQLSELAREHQVQSPALLIIGKVE"
  },
  {
    "reference": "METVITATIIGASILLAFAALGTAIGFAILGGKFLESSARQPELASSLQTKMFIVAGLLDAIAMIAVGISLLFIFANPFIGLLN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Key component of the F(0) channel; it plays a direct role in translocation across the membrane; A homomeric c-ring of between 10-14 subunits forms the central stalk rotor element with the F(1) delta and epsilon subunits..2. The designed protein should be involved in proton motive force-driven ATP synthesis by localizing to plasma membrane, proton-transporting ATP synthase complex, coupling factor F(o) and exhibiting proton-transporting ATP synthase activity, rotational mechanism, lipid binding.3. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "TRQPEMRPQLLVNTFIFAGLMF",
    "response#2": "GARQPEAGDQINRMLLFALAFIL",
    "response#3": "IGYGLAAIGPGVGVGIIFGNGTQAMARQPEAAGLIRANQILGFAFCEALALIGLVMPMLYA"
  },
  {
    "reference": "MKTPLAQPVVRPSDETPAIPLQMAPRRRGKPPRHFADLTPAERVASVVEAGEPAFRAKQLATHYFTHYTSDATRMTDLPARTRDDLASTMFPSLIRPTRTLKADGGTTVKTLWHLFDDAKVESVLMRYPQRTTLCVSSQAGCGMACPFCATGQLGLTRNLSTAEILEQVRAAFRSLADGEIPGGPARLNNLVFMGMGEPLANYKAVIETVRALVAPQPDGFGMSARNITVSTVGLVPAMQKLTKEGIPVTLALSLHAPDDDLRSELVPINTRWSVDEALDAARNYFEVTGRRVSIEYALIKDMNDHAWRADLLGEKLNARGKGWVHVNPIPLNPTPGSIWTASDREVEDEFVARLRGHGIPTTIRDTRGSDIDGACGQLAAEEDDE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein contains novel Radical SAM core domains that confer a unique function or activity.2. The [4Fe-4S] cluster, S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand.3. The protein can be expressed in cytoplasm and perform tRNA binding, tRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding without triggering undesirable cellular responses.4. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.5. [4Fe-4S] cluster is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "EKDRGTLCISSQVGCSLACTFCSTGMQGFNRNLTTAEIIAQIIIANEHLSHENKKISNVVFMGMGEPLLNEKSVYSACDLLLDDLAFGLSRRKVTISTSGVVPALYRMAQTTPVSLAISLHAPDDELRDELVPINQKYPIEELMAACKNYLTSGTQERHILFEYVMLDGVNDTMEHAKKLAKLLRGISAKVNLIPFNPFPKTQYKTSKAFTIKQFQDVLFQAGIRTMTRRTRGEDIDAACGQLVGESKPSLIGLDRVALADRLTDLGVPERQIRMRVAQLWNRLYVGGAQTFDDMTTLSKDLRHALDSQVSLDRPEIVSEQISTDGTRKWLLRLAPGRPGEAAPEIETVYIPEEDRGTLCVSSQVGCTLTCTFCHTGTQRLVRNLTPGEIVGQILIARDRIGEWPGATRPTDGPLPNAERLITNIVLMGMGEPLFNFDNVRQAMEIAADGDGISVSKRRITLSTSGIAPMIPRCGVELGVMLAISLHAVRDDLRDVLVPINRKYPIAELLDACRAYPSLSNAKRITFEYVMLRGVNDSLADAKALVRLLAGIPAKINLIPFNPWPGAPYESSEWEQIEKFAEIINKAGYASPVRTPRGRDIMAACGQLKSASEKMHARKERLAS",
    "response#2": "HGNHTTACLSSQAGCKLNCSFCATGQSGFARDLSAGEIVGQLLAMEKRLGANIGNVVFMGMGEPLLNREALFKAITILNSPKMRNMGARHITVSTAGIVPGILELAEFPVPVRLSVSLHAPNDALRNKLMPVNKKYPIGPLMDALKTYQQKTGERITVEYVMIRNVNDEPEHAYETAALFSGMGVYVNLIPYNPVTDRYQRSNPERIKVFSAALSRLGIENEIRKEKGTGRMEIVRL",
    "response#3": "EEDRGTLCVSSQVGCTLTCTFCHTGTQRLVRNLTPGEIVGQILIARDRIGEWPGATRPTDGPLPNAERLITNIVLMGMGEPLFNFDNVRQAMEIAADGDGISVSKRRITLSTSGIAPMIPRCGVELGVMLAISLHAVRDDLRDVLVPINRKYPIAELLDACRAYPSLSNAKRITFEYVMLRGVNDSLADAKALVRLLAGIPAKINLIPFNPWPGAPYESSEWEQIEKFAEIINKAGYASPVRTPRGEDIMAACGQLKSATERARKKP"
  },
  {
    "reference": "MVGKGVTVDTAMEEAGGETPRSPEPSFLATLGDPQVTLVSVHKRWSFRRNPGARGSSRPVTSEEEGECSQKVLNGTRGIRSNQILSKLSLPARAREPAMKQSFAFDNVGYEGGLDTVDPPQTATGTISISGMTCQSCVKSIEGRISSLKGIVSIKVSLEQGNATVRYVPSILSLPQICRHIEDMGFEASVAEGKAASWPSRSSPGLEAVVKLRVEGMTCQSCVSSIEGKLGKLQGVVRVRVSLSTQEAVITYQPYLIQPQDLRDHVSDMGFEAVIKNKVAPVSLGPIDIGRLQSTNPKTPLASDNQSLNNSETSEHQGSHTVTLQLRVDGMHCTSCVMNIEENIGQLPGVQSVQVSLESRLAQVQYDPSRVTATALQRAIEALPPGNFKVSLPDGMTGNGTGRRSSNRVVPAPTPRPQVQGVCDTVVLAIAGMTCASCAQSIEGLISQREGVQRISVSVADGTGVVLYDPSVTNPEELRAAVEEMGFEASVISENYSTNHVGNHSAGTSPAPPEAGVPVSVQEVAPCAGGLPKNHNSGSSSKPPQASTTVAPQKCFLQIGGMTCASCVSHIEKSLQKEAGILSVLVALMAGKAEVKYNPEVIQPLEIAQLIQDMGFEATVMEDYTGSDGDLELIITGMTCASCVHNIESRLTRTNGITYASVALATSKAHVKFDPEIIGPRDIVRIIEEIGFHASPAQRNSGAHHLDHKVEIKQWRKSFLCSLVFGIPVMGLMIYMLVPSHEPHEAMVLDRNIIPGLSILNLIFFILC",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have copper ion binding, P-type divalent copper transporter activity within perinuclear region of cytoplasm to regulate copper ion import, protein maturation by copper ion transfer.2. The designed protein's functionality will benefit from Polar residues, Basic and acidic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "VWFKDAAAMEETAHVDTVVLDKTGTLTKGELEISALWQPQSAVYSEDDLYRFAAAVERQANHPIAKAIVQAAEXKMLEIPTALFSKMEVGQGIQAELEQVGTIKVGKPDYCGLILPKNLEDIWQIASIVAVSINDEPIGAFALTDTLKNDSLHAIQRLQQQNIDVVIMSGDQQSVVDYIAKQLGIKKAFGKLTPRDKAEQIQKLKDLGHIVAMVGDGINDAPALASANVSFAMKSSSDIAEQTASATLMQHSVNQLVDALFIARG",
    "response#2": "GILVKGGEVLEASKKIDTVVFDKTGTLTRARMRVTDVIAGQRRQPDQVLRLAAAVESGSEHPIGAAIVAAAHERGLAIPAANAFTAVAGHGVRAQVNGGPVVVGRRKLVDEQHLVLPDHLAAAAVEQEERGRTAVFVGQDGQVVGVLAVADTVKDDAADVVGRLHAMGLQVAMITGDNARTAAAIAKQVGIEKVLAEVLPQDKVAEVRRLQDQGRVVAMVGDGVNDAPALVQADLGIAIGTGTDVAIEASDITLMSGRLDGVVRAIELSRQVWFKDAAAMEETAHVDTVVLDKTGTLTKGELEISALWQPQSAVYSEDDLYRFAAAVERQANHPIAKAIVQAAEXKMLEIPTALFSKMEVGQGIQAELEQVGTIKVGKPDYCGLILPKNLEDIWQIASIVAVSINDEPIGAFALTDTLKNDSLHAIQRLQQQNIDVVIMSGDQQSVVDYIAKQLGIKKAFGKLTPRDKAEQIQKLKDLGHIVAMVGDGINDAPALASANVSFAMKSSSDIAEQTASATLMQHSVNQLVDALFIARR",
    "response#3": "VWFKDAAAMEETAHVDTVVLDKTGTLTKGELEISALWQPQSAVYSEDDLYRFAAAVERQANHPIAKAIVQAAEXKMLEIPTALFSKMEVGQGIQAELEQVGTIKVGKPDYCGLILPKNLEDIWQIASIVAVSINDEPIGAFALTDTLKNDSLHAIQRLQQQNIDVVIMSGDQQSVVDYIAKQLGIKKAFGKLTPRDKAEQIQKLKDLGHIVAMVGDGINDAPALASANVSFAMKSSSDIAEQTASATLMQHSVNQLVDALFIARR"
  },
  {
    "reference": "SYIITTPRMWVAGSPAQVRTYVMPYGCGEQNMVNFAPN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that can perform serine-type endopeptidase inhibitor activity in extracellular region to support negative regulation of peptidase activity.2. The protein must exhibit the following characteristics: The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced); Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.; This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases; Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. The designed protein sequence is ",
    "response#1": "LGIHRLNGPLKCGQPQEVLVDYYIDPADASPDQEISFSYYLIGKGSLVMEGQKHLNSKKKGLKASFSLSLTFTSRLAPDPSLVIYAIFPSGGVVADKIQFSVEMCFDNQVSLGFSPSQQLPGAEVELQLQAAPGSLCALRAVDESVLLF",
    "response#2": "NGPLKCGQPQEVLVDYYIDPADASPDQEISFSYYLIGKGSLVMEGQKHLNSKKKGLKASFSLSLTFTSRLAPDPSLVIYAIFPSGGVVADKIQFSVEMCFDNQVSLGFSPSQQLPGAEVELQLQAAPGSLCALRAVDESVLLC",
    "response#3": "VHLEPVSHELPCGQTQTVQAHYILNGGALQGLKKLSFYYLIMAKGGIVRTGTHGLLVKQEDMKGHFSISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLANKVDLSFSPSQSLPALHAHLRVTAAPQSLCALRAVDQSVLLL"
  },
  {
    "reference": "MTDLTNQKDAALAAIAAATSLDALEEQRVAALGKKGWVSLALKTLGGMDPEERQKAAPAIQAVRAEVADAIAARKDALEREALEAQLATETLDLTLPAPQQRFGSVHPVSQVMDELAEIFADLGFAVATGPEIEDDWHNFTALNMAETHPARAMHDTFYFPDVDSEGRRMLLRTHTSPVQIRAMKAQGAPIRIIAPGRVYRSDSDATHTPMFHQVEGLVIDRDIHLGHLKWTLETFLKAFFEREDIVLRLRPSYFPFTEPSVEVDVGYSREGGRRVVGGDGNAEGHDWMELLGSGMVNRRVIEMAGLDPDEWQGFAWGLGVDRLAMLKYGMDDLRAFFDGDQRWLDHYGFSPFDQPTLSAGVGARS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.2. We are looking for a protein with broad substrate specificity for ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) +   L-phenylalanyl-tRNA(Phe).3. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.4. A protein that can perform ATP binding, tRNA binding, magnesium ion binding, phenylalanine-tRNA ligase activity in cytoplasm to support phenylalanyl-tRNA aminoacylation. The designed protein sequence is ",
    "response#1": "IDVTEPTTRGQRGAQHPITILSEQIADIFVGMGWEIADGPEVEAEYFNFDSLNFIPDHPARTLQDTFHIAPEGSGQVLRTHTSPVQMRTMLSRDLPIYIACPGRVFRTDELDATHTPVFHQVEGLAVDKGLTMAHLKGTLDHLAKTLFGEEAKTRIRPNYFPFTEPSAEVDVWFADKKGGAGWIEWGGCGMVNPNVLIAAGVDPEEYSGFAFGMGIERTLQFRNGLPDMRDMVEGDVRFISRTIEEMTAIFGAMGFSVAEGPDIESQWHNFSALNTPEHHPARTEHDTFYLPPKNEDGEPRVLRTQTSGVQIRTMLGSKPPIRIIAPGRTYRADHDATHSPMFHQCEGLVVDRNITLGHLKGCLIEFLRVYFDKPNLPVRFRASYFPFTEPSMEVDIGWSKATGEIGGGSDWLEVLGSGMIHPRVLANCGLDPAEWQGFAFGMGIERLAMLKNGIPDLRSFYESDLRWLRHYGFSAFLKS",
    "response#2": "IDVSLPGRPVSRGTLHPITRITRRICEIFGRMGFDVVEGPEIETDYYNFEALNIPKHHPARDMQDTFYISDNVVLRTHTSPTQPRVMEKTAPPLRIIAPGKVYRCDSDVTHTPMFHQVEGLMVDRNVSFGDLKGILTLFIHRMFDPETSLRFRPSYFPFTEPSAEVDIRCVACRGKGCRICSHTGWLEILGAGMVHPAVFEKAGYDTQAYTGFAFGMGVERIAMLKYNIDDTRKFFENDTRFLRQY",
    "response#3": "IDVTAPPIGTPFGHRHPLLTTTEHIVDLFCGLGYEVHEGPEIENDFYNFAALNIPEDHPARDMQDTFYLSNNLLLRTHTSPVQIRCLENSAPPVRIVAPGRVYRRDAVDATHSPVFHQVEVLAIDEGLDFSHLRGTVMAFLKAFFGDLPVRFRASYFPFTEPSAEVDVQWRGKWLEVMGCGMVDPSVLEGLGIDPERWSGFAAGLGVERFCMVRHGIDDIRRLYTSDLRFLEQR"
  },
  {
    "reference": "MRLIIVSGFSGSGKSIALDTLEDCGYYCIDNLPAPLIEPFLQQAIASRSSAFDKIAIGIDARNQSANLTGFPDILGRARQLGVDCRILFLQAEPETLLKRFSETRRKHPLTDASVPLAEAIELERHLLEPVLSRADLHIDTTYTTIHQLRELVRQRVGIPSGGLMSLCLQSFGFKHGVPMDTDFVFDARCLPNPHWTASLRPKTGKDPEVVAFLAESADVHDYLNHLTAFLERWIPCFLAENRSYLNVSIGCTGGQHRSVYLVEALAGRLNSDRYNLLVRHRELPEPAEESHGTK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a high affinity for ATP. The designed protein sequence is ",
    "response#1": "MPELPEVETIRRNLNTVLEGEIIREVLVYYRPIVSNDASFETKLKGQRIHKVEREAKYLKFILDDYLLVSHLRMEGKYFMDAPLNKHIHVVFNLSSGHTLSYEDTRKFGRL",
    "response#2": "MPELPEVETIRRNLNTVLEGEIIREVLVYYRPIVSNDASFETKLKGQRIHKVEREAKYLKFILDDYLLVSHLRMEGKYFMDAPLNKHIHVVFNLSSGHTLSYEDTRKFGRL",
    "response#3": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR"
  },
  {
    "reference": "MSSTDSVVAGQAKVTTWRKTDTRWVLGLFGTAIGAGVLFFPISAGIGGLLPIIFMLILAFPIAFFCHRALARLCLSGRSISDNITDTVDQHFGHVGGVVITFLYFFAICPLLWIYGVTITNTFIAFWQHQLLLPAINRGVVALAILLVMAFFIYFGKDLMVKVMGYLVFPFITCLVLISLSLIPYWTGDIFTSFDMHSLSLFGSHGILVTVWLGIAIMVFSFNFSPIVSSFVVSKREEYEADFGREYTEQKCAKIISRASVLMVVVVMFFAFSCLFTLSPQDMAQAKQQNIPILSYLANHFSSLGSGKSTYATVLEYGASIIALVAIFKSFFGHYLGTLEGLNGLIIKFGYHGEKSQAPMKKLNMISMVIIMGSTWVIAYINPNILDLIGAMGAPIIAALLCLLPMYAVWRVPALAKYKGKASNYFVTIIGLLTILNIVYQLM",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The protein should be optimized for plasma membrane-specific folding and stability, while maintaining threonine efflux transmembrane transporter activity, symporter activity.3. The protein should be designed to catalyze the following reaction H(+)(in) + L-serine(in) = H(+)(out) + L-serine(out). The designed protein sequence is ",
    "response#1": "HIQLIAIAGTIGTGLFLGAGKTIQMTGPSVIFAYILIGIAMFFFLRTIGEMLYNDPSQHSFLNFVTKYSGIRTGYFTQWSYWLVIVFVCISELTAIGTYIQFWLPHLPLWLIEIVMLALLFGLNTLNSRFFGETEFWFAMIKVAAILGMIVTAIILVASNFHYTTVLSGKTVNDTASLNNIFDGFQLFPHGAWNFVGALQMVMFAFTSMEFIGMTAAETVNPKKSLPKAINQIPVRILLFYVGALLAIMAIFNWHYIPADKSPFVIVFQLIGIKWAAALINFVVLTSAASALNSSLFSATRNMYSLAKQHDKGRLTAFTKLSKAGIPINALYMATALSLLAPVLTLIPQIKNAFNFAASCTTNLFLVVYFITLYTYWQYRKSDDYNPNGFLTPKPTIAVPFIAIIFAIVFASLFFNWL",
    "response#2": "HIQLIAIAGTIGTGLFLGAGKTIQMTGPSVIFAYILIGIAMFFFLRTIGEMLYNDPSQHSFLNFVTKYSGIRTGYFTQWSYWLVIVFVCISELTAIGTYIQFWLPHLPLWLIEIVMLALLFGLNTLNSRFFGETEFWFAMIKVAAILGMIVTAIILVASNFHYTTVLSGKTVNDTASLNNIFDGFQLFPHGAWNFVGALQMVMFAFTSMEFIGMTAAETVNPKKSLPKAINQIPVRILLFYVGALLAIMAIFNWHYIPADKSPFVIVFQLIGIKWAAALINFVVLTSAASALNSSLFSATRNMYSLAKQHDKGRLTAFTKLSKAGIPINALYMATALSLLAPVLTLIPQIKNAFNFAASCTTNLFLVVYFITLYTYWQYRKSDDYNPNGFLTPKPTIAVPFIAIIFAIVFASLFFNWARKE",
    "response#3": "HIQLIAIAGTIGTGLFLGAGKTIQMTGPSVIFAYILIGIAMFFFLRTIGEMLYNDPSQHSFLNFVTKYSGIRTGYFTQWSYWLVIVFVCISELTAIGTYIQFWLPHLPLWLIEIVMLALLFGLNTLNSRFFGETEFWFAMIKVAAILGMIVTAIILVASNFHYTTVLSGKTVNDTASLNNIFDGFQLFPHGAWNFVGALQMVMFAFTSMEFIGMTAAETVNPKKSLPKAINQIPVRILLFYVGALLAIMAIFNWHYIPADKSPFVIVFQLIGIKWAAALINFVVLTSAASALNSSLFSATRNMYSLAKQHDKGRLTAFTKLSKAGIPINALYMATALSLLAPVLTLIPQIKNAFNFAASCTTNLFLVVYFITLYTYWQYRKSDDYNPNGFLTPKPTIAVPFIAIIFAIVFASLFFNW"
  },
  {
    "reference": "MTPLLTSAQMRALEAEAIAAGRTTGRAMMEDAGRGVAQLILSRWPGARKARVLAGPGNNGGDGYVVARHLAEAGWRVEVAALAPPATADAAAAAADWHGPVRAWEDATGGADAETVVVDALFGTGLVRPLAAEVARVIDRLARQAGPVVAADIASGICADSGRVLGGGASHDVELTVTFQAARPGHLLDQGGAQSGELAVIDLGLDVGAEDGRRIMQVGAPRDMGKHTGHKYDHGHALILAGPATRGGAARLAARAALRIGAGLVTVGAPDDALIENAARLDAVMLREIGAPGQLRDALEDRRISAVLMGPGLGRDRASALVAAARGARALVLDADALDADLGALPAECVLTPHDGEFARLCPDLAARLAETPLQGPAFSRVDAAREAAARLGAVVLLKGPDTVIAAPDGRACIHAAHRAAAAPWLATAGSGDVLAGLITGLLARGFPAYDAAASATWLHAAAARRFGPGLIAEDLPDLIPAVLRDLGA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit ATP binding. The designed protein sequence is ",
    "response#1": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYV",
    "response#2": "LEEFDQIINSTPGFVKGMWCGERECEDLVKEKTGATARCMPLEQEQLSDKCMCCGKPAKSMVYWGKAM",
    "response#3": "LTERLCVRPTSETLFCEHYSNSIQSYRDLPKLYNQWCSVVRWEKTTRPFLRTLEFLWQEGHTAHATAEEAQEETIRMLNVYADVLENVLAIPVIKGRKTEKEKFAGAHATYTVESLMHDGKALQSGTSHNFGDGFAKAFDIQYTDKNNQLQYVHQTSWGVTTRLIGAIIY"
  },
  {
    "reference": "MKLKTLILAAGKGTRMKSELPKVIHEVNGIPMISKIIKVLEILKPEENILILGHKKEEVLKVVGENADYVVQTEQLGTGHAVLQAKDKLKDYDGDVMILCGDTPLLREETLKELYKFHKDTDSVTTILTSIYDNPFGYGRIVKENGLVKAIVEEKEADAEIKKIKEVNAGVYCFKGRELFEALSKITNNNEKGEYYLTDVIGIQVGEGKQVQSYVLSDNIEILGVNSKVELAQASKVLRDRKNIELMEKGAILIDPATVYIEEDVEIGRDTIIYPGAVLQGKTVIGENCQILGASRIVDSILRNNIKVESSVIEDSILENGVTIGPFAHLRPKSHLKEKVHIGNFVEVKKSVLEKGVKAGHLTYLGDAQIGEDTNIGAGTITCNYDGKNKFKTIVGKNSFIGSDSMLVAPVIIGENALVGAGSVITKDVPDNSLAVSRSKQIIKNDWRK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must bind to Mg(2+) in order to perform its enzymatic function.2. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+), UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA binding.3. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain..4. The designed protein must have at least one Nucleotidyl transferase domain.5. The Proton acceptor of the protein must be engineered to enable effective catalysis of acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate. The designed protein sequence is ",
    "response#1": "IILAAGQGTRMRSDLPKVLHPVAGKPMLQRVLETCKKLTNDRLAVVYGHGGEQVKQLTQEDGIVWVLQAEQRGTGHAVAQAIELAPDEAIVLIAYADTPLIRSETLQQLIAHLGDAALAVLTSKPVSPTGYGRIIRDKMGNVQKIVEEKDANDAEKQIREINTGIMVIPNKYLKTWLQNLKNDNAQGEYYLTDIVKMAVNDGINVATTQPTDMEEVEGVNNKLQLAALERKYQQNQARELMIAGVSIMDPAQTWIGIDVDLDRDVQLLPQTYLLGSTSVGSGTTVGPNVRVIDSRIGRDCLIEQAHIRGSAIEDGVNVGPFCSLRPGTVLRSGSKAGTFVEMKKTEVGEGSKVPHLSYMGDATIGRDANVGAGTITCNYDGVRKYPTEIGDKAFIGSDTMLVAPVRVGEGAVTGAGSAITKDVPPEGLGIERASQLIKEWKRPKKKK",
    "response#2": "VIILAAGKGTRMRSNLPKVLQPLAGRPLLGHVIETAKKLNAENIITIFGHAGNLVQQAFSHENIQWVEQKEQLGTGHAVKVTLPVLPTEGVSLILSGDVPCINPTTLEKLLSVSRETGIGLVTLTLDPADSYGRVIRDSDNEITRIVEQKDASAEELKIKEINSGVYCFESAFLKTALDKLSNNNSQNEYYLTDIIQILKDEGNRIRAFKVADENEALGVNDRVQLSEAESLMRKRINEQHMRNGVTFLSPDTAYISGEAQIGSDTIIMPNSIIEGKTVIGNGCIIGPNSRIVDSKIGSGTKVDNSVVTRSEIGADANIGPFAYIRPGCVLGDGVKAGDFVEVKNSVIGKGTKMSHLTYVGDADIGEGVNFGCGTVVVNYDGIKKHRSQVGDNAFIGCNVNLISPVKVGKGVFIAAGSTITDDIPDGAFAIARSRQRNIEGWVEKNRPNSKSAEAAKKAYQENKG",
    "response#3": "VIILAAGEGKRMKSNRAKVLMPLAGRPLLGHVLATARALRPAAIHIVYGHRGEQVRAAFADQPDLHWVLQTEQLGTGHAVEQALGGVPAEARVLVLYGDVPLTRAATLQQLVQAQDTQGMGRIITVDDATGAIAEIVEDRDCNDEQRRIREIYSGICCAPTAALKTWLARLDNNNAQGEYYLTDIVAMAVADGVPVVTAQPAQAWEVAGVNSRAQLAQLERRHQLNQAQALMEQRAELVRAEGIFQAKKRAEALENGVTMTAPETVFFGFDTYIGRDAVIEPNVVFGPGVTVESGATIRAFSHLEGCHVSRGAVVGPYARLRPGTELSENTKVGNFVEVKNATILEGAKVNHLSYIGDASIGARTNVGAGTITCNYDGFMKHRTEIGEGAFIGSNTMLVAPVKIGNEAMTATGSVINKDVPDGALALARARQXTIKGWQRPXKKPXAAX"
  },
  {
    "reference": "MNTPLSIVILAAGKGTRMYSDLPKVLHKIAGKPMVKHVIDTVKAIHAKQIYLVYGQGGEVMQQHLADEPVHWVHQAEQLGTGHAMQQAAPFFQDEENILMLYGDGPMISETTLQRLIAAKPENGIALLTVVLENPTGYGRIVRENGKVVAIVEQKDANAEQLKIQEINTGVLVANGKQLKTWLAQLTNNNAQGEYYMTDVIALANQAGSDVVAVQAEDFMEVEGVNNRQQLARLERYYQRKQAEKLLLAGVALADPERFDLRGELTHGKDVEIDVNVILEGKVQLGNRVRIGAGCVLKNCVIADDVEIKPYSVLEDASVGQASQIGPFSRLRPGAELSEQTHIGNFVEIKKSQVGKGSKVNHLSYVGDSSIGKDCNIGAGVITCNYDGANKFKTIIGDDVFVGSDVQLIAPVTVENGATIGAGSTISKNVNADELVLTRVPQRHIKDWQRPKRQK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be able to regulate cell morphogenesis.2. The designed protein have MobA-like NTP transferase domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "GVRLVDPARFELRAAPGSALPGALTCESDVEIDIGCIFAGDVRIGSGSRIGAYCHISQADLGADCEVLPYSHIDGEAAGVVVGNGARIGPFARLRPGARLAEDVHIGNFVEVKNSTLARGAKANHLAYLGDAAVGERVNYGAGAITANYDGANKHRTIIEADVHIGSNSVLVAPLTIGAGATVGAGSTLSRDVPAHALAVTRR",
    "response#2": "GVTIMDPSSTYIGCAVEVGADSIIYPGTILEGKTVIGQDCLIGPGVRIVDSTIGSNSQIQNAVILESKVGNNCSIGPFAYLRPGTILEDNVKIGDFVEIKKSVIGRGSKVPHLSYIGDAFLGEKVNVGCGTITCNYDGKNKHITRIEDGAFIGSNTNLIAPVKVGKNAVIGAGSTINKDVPANALGIAREKR",
    "response#3": "GVSILDPASTWIDTAVELDEDVTVLPGTQLHGTTVIARDAVIGPDTTLTDVVVGEGAHVVRTHGSGSRIGAGASVGPFTYLRPGTVLGEDGKIGAFYETKNVTVGRGSKLSHLGYAGDAEIGEYTNIGCGNITANYDGVDKHRTVIGSHVRTSSNTVFVAPVTVGDGAYTGAGAVVRKDIPAGALGLSIAPE"
  },
  {
    "reference": "MASRKENAKSANRVLRISQLDALELNKALEQLVWSQFTQCFHGFKPGLLARFEPEVKACLWVFLWRFTIYSKNATVGQSVLNIKYKNDFSPNLRYQPPSKNQKIWYAVCTIGGRWLEERCYDLFRNHHLASFGKVKQCVNFVIGLLKLGGLINFLIFLQRGKFATLTERLLGIHSVFCKPQNICEVGFEYMNRELLWHGFAEFLIFLLPLINVQKLKAKLSSWCIPLTGAPNSDNTLATSGKECALCGEWPTMPHTIGCEHIFCYFCAKSSFLFDVYFTCPKCGTEVHSLQPLKSGIEMSEVNAL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.2. The ideal protein would be one that plays a critical role in protein import into peroxisome matrix through ubiquitin protein ligase activity within Cdc73/Paf1 complex, membrane, cytosol, peroxisomal membrane.3. The protein should contain Helical; Name=TM1 transmembrane regions to facilitate transport across the membrane.4. The protein should be designed to have high stability and activity towards S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +   [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-   cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.. The designed protein sequence is ",
    "response#1": "MVAFTATGNRNLTRKIRVTIVAADSLIKRDILRLPDPFAIVSVDSEQIHTTSVIKRTLNPYWNENFDIDVKDSSIVAVQIFDQRKFKRKQDQGFLGVINIKVSDVIDLELGGQEMLTRQTPNDLKKRLMIKFEGEDGLDYGGLSREFFFLLSHEMFNPFYCLFEYSAHDNYTLQINPNSGVNPEHLNYFKFIGRVVGLGIFHRRFLDAYFIVSFYKMILGKKIALQDLESVDAGLFRGLTWMLENDITGVIEDTFSITEEHFGEVVTVDLKPGGRDVEVTEDNKKDYVDLVTEYRISKRVSEQFQAFMSGFNELIPQELINVFDERELELLIGGMSEIDVDDWQKHTDYRGYNPSDEVVEWFWKIVKNWPAEKKSRLLQFTTGTSRIV",
    "response#2": "MVAFTATGNRNLTRKIRVTIVAADSLIKRDILRLPDPFAIVSVDSEQIHTTSVIKRTLNPYWNENFDIDVKDSSIVAVQIFDQRKFKRKQDQGFLGVINIKVSDVIDLELGGQEMLTP",
    "response#3": "MVAFTATGNRNLTRKIRVTIVAADSLIKRDILRLPDPFAIVSVDSEQIHTTSVIKRTLNPYWNENFDIDVKDSSIVAVQIFDQRKFKRKQDQGFLGVINIKVSDVIDLELGGQEMLTVWEMRLTSTARVYFVDHNTKTTTWDDTEDSFTWNIPDWNELTNPKYNSPRFRIGDFEWDILLFPQGNHNKGVAVYLEPHPEEKLDETTGEMVPVDPDWYCCAQFAIGISRPGNGDTINLINKSHHRFNALDTDWGFANLIDLNNLKHPSKGRPLSFLNEGTLNITAYTHLWNDYSKIIHQRTNTVPFDLVPHEDGVAVAVRVLKPLDSQDLGLETVYEKFHPSIQSFTDVIGHYISGERPKGIQETEEMLKVGATLTGVGELVLDNNSVRLQPPKQGMQYYLSSQDFESLLQRQDSSVRLWKVLTLVFGFAS"
  },
  {
    "reference": "MNFNHFDESGNAVMVDVSHKQPTLRTAVAAARVGMSPELLAAIREGGMAKGDVLGVARLAGIMAAKKTPDLIPLSHPLAIHSVAVDYELDSGAGTIQVRCTVRALERTGVEMEAMTGASLAALTIYDMCKGSDKSITIGDIRLLYKEGGKSGVYRREEGQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must be able to cyclic pyranopterin monophosphate synthase activity.2. The designed protein must have a active site that is structurally stable and resistant to denaturation.3. A protein that can modulate the overall flux of metabolites through the molybdopterin biosynthesis, Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed.4. The designed protein should have a binding site that is specific to the target substrate ligand. The designed protein sequence is ",
    "response#1": "MVDVAAKPATHRVAVATGRIEMQPATLALIESGTAKKGDVLGIARIAGIQAAKKTSDLIPLCHPLALTRVALAFALAEKGNAPQVVCTATVETVGPTGVEMEALTAVQVALLTIYDMCKAVDRGMRITDVHVLEKHGG",
    "response#2": "MVDITEKEDTIRVAVAQTSVTVNKEIYEKMTNRMIEKGDVLAVAQVAGIMAAKKTSDLIPMCHPLMLKGVDIQFAWHVEEEKALYQLLITVTVKTKGSTGVEMEALTAASVCALTVYDMCKALDKGMVIGPTYLVEKSGG",
    "response#3": "MVEVGHKDVVFRKAVAKGRIRLKPETIELIKAGKTKKGNVIAAAQIAGILAVKKTPELIPLCHPIPLTGVDITFEFGDDYIEATCEVRAYYKTGVEMEALTGVSVALLTIWDMVKAVEKDENGQYPFTRIEDIRVVEKVKL"
  },
  {
    "reference": "MSTLPNIRGLDQDQLKAWMTEIGEKPFRAKQIYEWIWKKNVSSFSEMSNLGKGLRNQLENTFSLGKVTIDDAQKSQDGTIKFAFRLDGGHLVEGVLIPTDSRITACISCQAGCSLSCKFCATGYLDLKHNLKAYEIYDQVDIIRQTALKEYDRPLTNIVYMGMGEPLLNYRNVMQSIQMISSEDGMGMAPRRITVSTAGISKMIRKLGDDGARFEFALSLHAANDEKRSKIMPINEHNSLEALGDALKYFYEKTHTRVTYEYIIFQDFNDSLEDAEELYQFCQVIPCKVNIIEYNPIDDAEFQNTSGNRMYRFKDYLEEKGVIVNIRRSRGRDIDGACGQLALKKR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit tRNA binding.2. The designed protein must have a S-methylcysteine intermediate that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "ISTQAGCALECAFCSTGRQGFSRNLTTGEIIGQLWWAKKALGDFRDAGSRETGRRIAEADFPVDRIISNVVLMGMGEPLANFESVVAALHLMLDDNAYGLSRRRVTVSTSGILPAMDRLRDECPVALAVSLHAPNDALRDRLVPINRKYPLAELMATCRHYLDKAPRDFITFEYVMLDGVNDSEEHARER",
    "response#2": "SSQAGCGMACPFCATGQGGLTRNLSTGEIVEQVRMGMVAAESGDLAGGPTHVSNIVFMGMGEPLANWRQVQAALHRMIEPSPSGLGISARGVTVSTVGMVPLIDKLADEGLPVTLAVSLHAPDDGLRDPLIPLNSRFNVKKLLDSARRYFEKTGRRVSIEYALIKDMNDHQY",
    "response#3": "RDGRRTVCLSSQSGCPLTCTFCATGQMRFGRNLTASEMLDQALHFRRLETIDHVVFMGMGEPMLNVDEVIAAARRLPDLGVTHRRTTVSTVGWLPGLRKFVDEVDEPVRLALSLHAAEPALRSQLMPVNDRYPLADVLAECRRYFELRSRKVFIEYVMLAGVNDRVEQARSLAELLDPQVFKVNLIPYNPTGSFTGSSPKAIAAFEAELKRRGIPTTVRLTRGRH"
  },
  {
    "reference": "MTSIVLGVSAGIAVYKSCELLRLFTESGHDVRVVPTRDALRFVGEPTWAALSGNPVTTEVWESVHEVPHVRIGQSADLVVVAPATADVLARAAHGMANDLLTNTLLTARCPVVFAPAMHTEMWEHPATRANVATLRERGAVVIEPATGRLTGADTGPGRLPDPAEIFEVCRRVLAGRGGDLAGRHVVVSAGGTREAIDPVRFIGNRSSGLQGYALARTAVARGAEVTLVSANVALPDPAGAKVVRVESATQLREAVLGAAEHADAVVMAAAVADFRPVRQSGTKIKKSAAEPDPIHLTKNPDILAELGETRRAGSGTYPRVIVGFAAETDDVLANGRAKLERKGCDLLVVNQVGENLAFGTPDNAAVVLTAEGDSVEIPRGPKEDLADVVWNLVVRRLP",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in coenzyme A biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.2. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A The designed protein sequence is ",
    "response#1": "LRGDRVVVSAGGTREPLDPVRFLGNRSSGRQGLAVAQAAVARGAEVVLVAAHLDADVAAEAAGVVGLRVVPVGTAAELATAMDEAAKEASVVVMAAAVADYRAAEIAERKIKKDDTGDELILRLVRNPDVLAGLAEAAVSGRLIVGFAAETASDRDELLALGRSKIARKGADLLVLNRVGWTDGFGTPDNEVLVL",
    "response#2": "IIGLAGSVAVGKSTTARLLQKLLKAWPEHHHVDLVTTDGFLYPNETLEARGLMDKKGFPESYDLPALIRFLSDVKAGEPYVKAPVYSHLTYNIVEGDYQVVHEPDIVIVEGINVLQVNKRNHHIPNVFVSDFFDFSIYVDAKEEQILQWYIEK",
    "response#3": "VIGVAGSVAVGKSTTARVLQALLARWDHHPRVDLVTTDGFLYPNAELSRRNIMHRKGFPESYNRRGLLRFVTEVKSGAREVTAPVYSHLLYDIIKNEKHVVQQPDILILEGLNVLQTGPTLMVSDLFDFSIYVDARIEDIEQWYISRFLAMG"
  },
  {
    "reference": "MKKSALQKARAAYRPKLPIVLTGAVKAVEGHPTQSVADQEEIQKLFPNTYGLPELHFEKNPNPGAPKAVNVGVILSGGQAPGGHNVISGLFDGIKKVNRNSRLYGFLMGPGGLVEHRYMEITSEIIDEYRNTGGFDIIGSGRTKLETKEQFDKGLEILRELNISALVIIGGDDSNTNAAVLAEYYKSIKAGVQVIGCPKTIDGDLKNDVIETSFGFDTATKVYSEVIGNIQRDCNSSKKYWHFIKLMGRSASHIALECALQCQPNICIISEEVEANNSTLQDVVNYIADIVAKRAENGNNFGSVLIPEGLIEFIPAVKALIAELNDLLANKAEEFAALTTEEEQRQFVLSNLSEANAATYASLPAGVARQLTLDRDPHGNVQVSLIETEKLLSAMVATRLEEMRAAGNYNGKFTAMHHFFGYEGRCADPSNFDADYCYGLGYNAACLISAGVTGYMSSLRNLTKPSVQWIAGGIPVTMMMNMERRHGEMKPVIQKALVKLDGAPFLKFASKRDDWAYNTCYTYPGPIQYFGPAEVCDQPSLTLKYEHQGK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should interact with enzymes and substrates within the Carbohydrate degradation pathway to enhance or regulate its activity.2. The designed protein must be optimized for metal ion binding.3. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis; Consistently, PPi-PFK can replace the enzymes of both the forward (ATP- PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.. The designed protein sequence is ",
    "response#1": "RVGMLTGGGDCPGLNAVLRAAVRKGVQRYGYDFLGFRDGWRGPLEGDTVRLDIPAVRGTLPRGGTILGSSRTNPLQAADGVRRIRENLAKYEVDALIAIGGEDTLGVAARLTDEYGIRCVGVPKTIDNDLSATDYTFGFDTAVGVATEAIDRLHTTAESHMRVLVVEVMGRHAGWIALHSGLAGGANCVLIPERRFDVDEVCDWVTSRFRASYAPIVVVAEGATPKGGEMVLKDESTDSFGHVRLSGVGEWLAKRIEERTGKEARTTVLGHIQRGGTPSAFDRWLASRFGLHAIDAVKDGKFGTMVALRGTEIISVDLGEATAELKTVPLERYEEATRIFG",
    "response#2": "IGVLTGGGDAPGLNAVIRALTVKAMSLGYNVLGFRKGWNGLLGEGEPWPLDLDNVEGIHTLGGTILGTSRTNPYKIENGVEQLKQNLKKFNVHCLVAVGGEDTLGVAMKLQNEGVKVVGVPKTIDNDVNATDYTFGFDTAVNIAAKAIGDLHTTAKSHERVMVVEVMGRHAGWITLHAGIAGGAHYIALPEEPYDIEEICKIVKQRDKQGKHYSIIAVSEGVVSKQTSTVQTQGKALDAFGHVRLGGVAETLAAEIEKLTGKESRHVVLGHLQRAGQPSAFDIVLGTRFGIHAAEGVVAAGRFENVVVAQSGGPTCVINNSLRGIVETCFRLPGQFGTVFAGRFGIEGILKEELLDLSATPAEEIALLRTSPAAGGIGTCRYKLKPGQDEDFARVVEVLKAHDVGYFFYIGGNDSQDTAHKVAQLAHDRGLDLVATGVPKTIDNDLGDPEFTLLDHSPGYGSVARYMAQYIRQVNEENAGSSPADPVMVIQAMGRKIGFIPAAARLADPNREMPLQIYLAEAKVSLEQLGKNVVEALREFGRCIVVVSEGFDVGDLGASKDTFGHVQFSASQTTVAQVVTNYINSLDLPVPGKARFQIPGTDQRNAIAYASVVDLDEAYRVGCHAVDVALNGEVGYMAALHG",
    "response#3": "QIGVLTGGGDAPGLNAVIRGITIKAISLGYDVLGFRKGWAGLLGEGEHHPLDLDEVEDIHMLGGTILGTSRTNPYKVENGLEQVKKNLRKFKCDALIAIGGEDTLGVAWKLYKDGLNVVGVPKTIDNDVNATDYTFGFDTAVNIAADAIDRLHTTAKSHSRILLVEVMGRHAGWLTLHAGMAGGAHIILIPEEPFDVNEICKIIDRREKSGKSYTIIAVSEGALPKEGDMTLQCKEKDEFGHVRLGGIAECLARDITKLTGKECRHVVLGHLQRAGHPTAFDRVLGTRLGLHAAELVHEGRFGMMAALRGERIVDVPLTEATAELKTVPPELYEEAEVFFG"
  },
  {
    "reference": "MARVKEEADRISEQANSHIENIRRQIEQEKNDWAAEKQKLIEEAKAEGFEQGVALGKAEAMKQYAELIGQANSITEMSRKAVEDKLEDANEEIVELAVALAKKVWQQKSDDKEAFLLLVQQVINEVKEYDDISIYVDPYYYETIFQQKDEIQQLLYKECRLGIYADEKAQKGTCYIETPFGRVDASVDTQLMQLKDKLLTALEAGAAE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Needed for flagellar regrowth and assembly..2. The designed protein must be able to regulate bacterial-type flagellum assembly. The designed protein sequence is ",
    "response#1": "KFTHATHATSIDVIDSLGTKHAMRIEFYRSGGAEWNFRVIVPEPGELVGGSAARPNVFEGGRLHFNNDGSLAGMNPPLLQFDPKNGADAPQRINLAFGSSGSFDGLTSVDKISETYAIEQNGE",
    "response#2": "YLLYGSREIRQILQGLIDHHALITAQTVPGHQSFLTTVVALPDDGASIIIDAGPDEHINQRVGNAERLVCMSQLDKIRIQFDLSAPALTRYENRPAFRAPVPAQLLK",
    "response#3": "YLLYGSREIRQILQGLIDHHALITAQTVPGHQSFLTTVVALPDDGASIIIDAGPDEHINQRVGNAERLVCMSQLDKIRIQFDLSAPALTRYENRPAFRAPVPAQLLF"
  },
  {
    "reference": "MARELSATTKAIVKATVPALAAHGTDITAAMYLLLFKNEEVRKLFNQSNQGEGGGQTKALAQAILAYAQNIDNLAVLGPAVERIAQKHVGLQIQPHHYPYVANALLAAIKQVMGEAATDEVLAAWGEAYWFLADILIGREKQVYEELSSAEGGWNGWRRFRIVGKQRESDIITSFVLEPEDHEAVVRHLPGQYLTFRIAVPGKGEYRRNYSISSAPNGRTYRISVKREPSGVISNYLHGSVVEGDVLDVAPPAGDFFLKEDTTRPIVLLSGGVGLTPMISMLEAMAAKDGNASVYYVHGAEDGRVHAMGEHVRKLILGREGMRSAIFYRTPHSEDRKGVHYDQEGFVTLEWLAENTPLKEADIYLCGPKPFLAVFVNGLAKAGIPNDRIHYEFFGPADNLAA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein have FAD-binding FR-type domains with specific sequence motifs that are crucial for its activity.2. For general function, the protein need meet that Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds; Therefore, plays a central role in the inducible response to nitrosative stress.3. The designed protein should have a binding site that is specific to the target NADP(+) ligand.4. The protein must exhibit FAD binding.5. The Charge relay system of the protein must be engineered to enable effective catalysis of NADPH + 2 nitric oxide + 2 O2 = H(+) + NADP(+) + 2 nitrate. The designed protein sequence is ",
    "response#1": "MLSTEHKAIITATVPLLETGGEALTRHFYQTMFRDYPEVKPLFNQANQHEGHQQRALANAVLMYAKNIEKLENLGPLVSTIINKHVSLQIQPEHYPIVGASLLKAIREVLGEEIATDAVIEAWATAYGQLAEILKVPESSEITSFYLAPADGGAVPDFKPGQYVSVRVHVPALGMMQPRQYSLSDAAGQGRLRISVKRERGAAGSPDGMVSGVLHAQFDEGDVLDVAPPQGDLFLEARGARWFRLAGKVAESEEIVSLHLQPEDGGALLEFHPGQYIGLRLHLDGEEERRNYSLSALGDGREYRISVKREAGGKVSNYLHDQLQVGDRLELFPPAGDFVLDRRDRPLVLLSGGVGLTPMVSMLESLAERDPSRPVTFLHAATSGSEHAMGAEVRRLAERHPRFRVHLSYARPTEGDLHGRDYDREGLVDLEAVREVVGDDGADAYVCGPVPFMRMQHDALVALGVPAERRHYEFFGPAASLQP",
    "response#2": "MLSTEHKAIISATVPILEQGGETLTRHFYQSLFRDYPGVKPLFNQSNQQDGNQQRALANAVLMYAKNIEKLENLGPLVSTIINKHVSLQIQPEHYPIVGASLLKAIREVLGAEVATDAVLEAWGAAYGQLADILRGWRDFAVVEKHQESEIITSFVLRPVDGGAVVRHKPGQYLTLWLDIPGEHPLKRNYSISAAPNGETYRISVKREPQGIASGWLHDHVEVGATLKVSVPAGEFFLE",
    "response#3": "MLSTEHKAIISATVPILEQGGETLTRHFYQSLFRDYPGVKPLFNQSNQQDGNQQRALANAVLMYAKNIEKLENLGPLVSTIINKHVSLQIQPEHYPIVGASLLKAIREVLGAEVATDAVLEAWGAAYGQLADILFRVMDKQIESAEITSFYLQPTDGGKLPKFHPGQYVTVQTYIPSLGLTQPRQYSLSDAPGKETFRISVKREDAINMRPAGTVSNLLHNTINVGDILQLTPPAGDGQD"
  },
  {
    "reference": "MDLSAEFKRWKAQCLSKADLSRKGSVDEDVLEIVQLLNGQEQFFTTSSCAGRIILLDRSVNGSEVQKQNCCWLLVTHKACVKDDVIVALQKAKGDAILKFEPLVLHVQCRQLQDAQILHSVAIDSGFRNSGITVGKRGKTMLAVRSTHGLEVPLSHQGKLMVTEEYINFLLKIANQKMEENKKRIERFYHCLQHALEKETVSTTSQPKEKVNTSYIRKKKRNPGKARGKRVNEEHDKELENNDHDDPGISDTIFPEDY",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that is capable of catalyzing reactions within the tRNA modification pathway with high efficiency and specificity.2. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.3. The designed protein should have tRNA methyltransferase activity to facilitate tRNA methylation.4. The protein should have a fast turnover rate for 4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in   tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-   carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-   homocysteine.5. The protein must exhibit the following characteristics: Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA .; Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. The designed protein sequence is ",
    "response#1": "WQRHGDLLLLSEDCFQAKQWRHLEPELWETVASALGAQRLAKRGRVSPDSTRTPAVSLLLGDHGWVEHVDNGIRYKFDVTQCMFSFGNITEKLRVASLPCVGEVLVDLYAGIGYFTLPFLVHAEAAFVHACEWNPHAVVALRNNLELNGVADRCQIHFGDNRKLKLSNVADRVNLGLIPSSEEGWPIACRVLKQDAGGILHIHQNVESFPGKTLQPPGSSEMEEHWPSPHQIISNQLNNGATSDSRRKTLSVATKPEWQRWAKAAETRIATLLHQVHGKRWKTQILHIQPVKSYAPHVDHIVLDLECREMPQLDFETVGHIAHLNLRDRYQPFKHIIGQVILDKSSHIKTVVNKLDNIDHTFRFFQMEILAGINDMNAKLKEGGCFFHFDFSKVYWNSRLQGEHDRIIKLFGQNDLICDVFAGVGPFALPAAKHKRCVVFANDLNPQSFKYLMENIKLNKLETRILPFNMDGRQFIKQSLEDLNNPAIWNKITKQKPTSNDKKRNRKVESPTVAPLTDQPAISGIRHFKHYVMNLPATAIEFLDAFHGLYSGMRDVIMDSDLPTIHCHCFSNAKDVKADVIERVERVIGMPLGSNLIMVHSVRTVAPNKDMLCISFRLPQLQKN",
    "response#2": "WQRHGNLLLLSEDCFQAKQWKNLGPELWETVALALGVQRLAKRGRVSPDGTRTPAVTLLLGDHGWVEHVDNGIRYKFDVTQCMFSFGNITEKLRVASLSCAGEVLVDLYAGIGYFTLPFLVHAGAAFVHACEWNPHAVVALRNNLEINGVADRCQIHFGDNRKLKLSNIADRVILGLIPSSEEGWPIACQVLRQDAGGILHIHQNVESFPGKNLQALGVSKVEKEHWLYPQQITTNQWKNGATRDSRGKMLSPATKPEWQRWAESAETRIATLLQQVHGKPWKTQILHIQPVKSYAPHVDHIVLDLECCALDDDMV",
    "response#3": "QKKEILEGLKSSVPDASPKGHPDSPIFPLLDVINSHPDWVTTSSCSGRISVYVQGANSRKGGGYWLFVSHQAHEELPPVLEDEKVEYGKVPSSPVEGNREIQYAFEPMILHVQTRSLANAQHLQRVAASCGFRETGIQGSEQKFIVAIRTSLRMDIPIGCLTASEKLQFYITREYMCFLFKRSVEYFTENGNRMARLKEQLS"
  },
  {
    "reference": "MLWTDETLKMKEDEHSTQKSQDEILSDFSSPIFEFNDSTFLSDEDLIMSAINGNGLRKRKNRSFSISDDNLAKQEPSKNGKVEKTREIPEYGMKKCHSARASLFSSSSGFDDYRGFLNLCVVLLAVTNARVFLENIMKYGILVDPVQWVATVLDNPYKWPNLLLILGSNVFILCAFHIEKMLLKEFISATTCMYLHILNLLLLILLPPFRILQQPADPVGSLSSCGVYVILFLKLWSFAQANKWYREDYLKSQGKRKLQRTMSMPSSALKSLRDGTETIEVKYPYNLRYRNMYYFILAPTLCYETQYPRNKSINKVYLARRLVETVFLVQLGLALVQQWIVPVLQKAIMPIHNKEGYYVVERLLKLAIPNHFIWLIFFYCMFHSILNVIAEVLRFADREFYRDWWNSETIDYFWKAWNIPVHKWCLRHVYKPLLSVGYKKFTAQLCVFLLSAFFHEYLVSIPLHLFRTWAFMGMAMQIPLSVFTILITKRFSGHYGNMIVWMSLILGQPIAILAYVYHYYIIQYAPNAQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must be optimized for retinol O-fatty-acyltransferase activity. The designed protein sequence is ",
    "response#1": "EPHFNEPYLATSLQDFWGHRWNLMVSAIYRPGVYSPVRSVCQHQMRSDWARFMGCMTTFFVSGLIHELVYFYINREKPTLEVTWFFG",
    "response#2": "EPHFNEPYLATSLQDFWGHRWNLMVSAIYRPGVYSPVRSVCQHQMRSDWARFMGCMTTFFVSGLIHELVYFYINREKPTLEVTWFFG",
    "response#3": "EPHFNEPYLATSLQDFWGHRWNLMVSAIYRPGVYSPVRSVCQHQMRSDWARFMGCMTTFFVSGLIHELVYFYINREKPTLEVTWFFG"
  },
  {
    "reference": "MRKVNLAIAGATGLVGNKIMEILKERNFEYDNLYLFSSKKSAGQIINFNGKDYKVEELTETSFDDKDIDIALFSAGGTTSLKYAPIAAKNNIVVIDNSSAWRMEKDIPLIVPEVNPDTAFNNKGIISNPNCSTIQSVVPLKPLYDKFGIKRIVYNTYQAVSGSGLAGIADLENRTCNKYPYPIAKNCLPHIDDFLENGYTKEEIKMVEETKKILEDDTIKITATTVRVPIKNSHAVSMNIEFEKPFEIEEVFEVLKNAPGIVVKDDIDNLLYPLEEDTNGKDDVYVGRIRRDYSIKNGINLWCVADNIRKGAATNTVQIAELIASRL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A key design requirement for the protein is the optimization of the Proton acceptor, Acyl-thioester intermediate to achieve high catalytic efficiency for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.2. A protein with aspartate-semialdehyde dehydrogenase activity, NAD binding, protein dimerization activity, NADP binding functions is required to enable isoleucine biosynthetic process, threonine biosynthetic process, 'de novo' L-methionine biosynthetic process.3. The protein contains novel Semialdehyde dehydrogenase NAD-binding domains that confer a unique function or activity.4. The protein design should take into account the physiological conditions of the L-threonine biosynthesis, Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5 pathway.5. The designed protein should be able to compete with endogenous proteins or inhibitors for NADP(+), phosphate, substrate binding.6. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.. The designed protein sequence is ",
    "response#1": "MNLGIVGATGQVGGKVIELIEQRGLAVDELRLFASSRSAGSQLVVGGERITVEDAATADPTGLDVAIFSAGATTSRAQAERFAAAGVTVVDNSSAWRMDPDVPLVVSEVNAHALDATARLPKGIIANPNCTTMAAMPVLRPLHAEAGLARLVVATYQAVSGTGLAGVEELLGQLQAAVAQHPERLTHDGHALDLPEPVKYVAPIAMNVLPLAGSIVDDGEGETDEEKKLRNESRKILEIPDLLVAGTCVRVPVFTGHSLSIHAEFDRPISPERARELLADAPGVVLADVPTPQDAAGTDPTIVGRIRADQSAPEGRGLVLFLSSDNLRKGAALNAIQIAELIATLAVVGATGAVGTVLLQILSQRADIWGEIRLVASPRSAGRKLAVRGEQVEVTALSEEAFDGVDLALFDVPDEVAAQWVPVAAARGVVVVDNSGAFRMDPDVPLVVPEVNPHAVRVLAPIKAKYGLTRVIVSTYQAASGAGQSAWEELVAETTAHNAGQEMHANILPVKNAEHHYPLAYNLLPQIDVFEDDGYTHEEWKMIHETKKIMFGDKNSKAVKVTGTAVRVPVPIGHGEAVYFETEAGTGATSGDIQSILSKAPGVVLEDNPKEQLYPQPINAEGKRETFVGRVRPDLENDDSFHMWVVSDNLLPVPQ",
    "response#2": "MAVAILGATGAVGTRLIEQLEQSTIPVKSIKLLASSRSAGKILQFQGQNVVVEEATPESFTGVDLVLASGGGSVSARFAPEAIKRGAVVVDNSSQWRMNDNVPLVVPEVNEEALKNAPKGIIANPNCTTMAAMPVLHPLHRAFGVTRLFASSYQAVSGAGARAIDELRHQTEALAKGRPMVKEIFPHQIAFNVIPQVDSFLDTGYTKEEMKMENEGRRIMHLPDFKASVTCVRVPVYRAHSVAVSAEFAKPISLEEARTALKNAPGLQFVEDGYPMPLDCAGKDDCFAGRLRRDCAMENGIAFWVCGDQLLKGAALNAVQIAELVAAELG",
    "response#3": "MALGIVGATGQVGGKVIELIEQRGLAVDELRLFASSRSAGSQLVVGGERITVEDAATADPTGLDVAIFSAGATTSRAQAERFAAAGVTVVDNSSAWRMDPDVPLVVSEVNAHALDHHRRADRIIANPNCSTIQMVVVLGALHAAYGLRELVVSSYQAVSGAGRAGVETLRRQIALVAGTELGTSPGDVRRAVGEDTGPFPEPVALNVVPWAGTPREDGWSSEEMKVRDESRKILGLPKLPVAVTCVRVPVVTTHSLTVHARFEDTVTVDGAREIIATAPGVVLFDNPEAGEYPTPADVVGTDPTWVGRVRRALDDPTALELFVCGDNLRKGAATNTIQIAELLV"
  },
  {
    "reference": "MLYANPNTESSPVGFRKKYDNFI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein with aldehyde dehydrogenase (NAD+) activity, glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity functions is required to enable alcohol metabolic process. The designed protein sequence is ",
    "response#1": "LAPFAKVLNEKFGVKRGMMTTIHSYTNDQQILDLPHKDLRRARAAAENMIPTSTGAAKAVALVLPELKGKLNGGAVRVPTANVSLVDLVVELDKEVTVEEVNAAFKAAAEGELKGILGYSEEPLVSIDYNGCTASSTIDALSTMVMEGNMVKVLSWR",
    "response#2": "LAPFAKVLNEKFGVKRGMMTTIHSYTNDQQILDLPHKDLRRARAAAENMIPTSTGAAKAVALVLPELKGKLNGGAVRVPTANVSLVDLVVELDKEVTVEEVNAAFKAAAEGELKGILGYSEEPLVSIDYNGCTASSTIDALSTMVMEGNMVKVLSWR",
    "response#3": "LAPFAKVLNEKFGVKRGMMTTIHSYTNDQQILDLPHKDLRRARAAAENMIPTSTGAAKAVALVLPELKGKLNGGAVRVPTANVSLVDLVVELDKEVTVEEVNAAFKAAAEGELKGILGYSEEPLVSIDYNGCTASSTIDALSTMVMEGNMVKVLSWR"
  },
  {
    "reference": "MAADDIVGCYQVDKARRSLTNVESYPKVGEQLHGFTVQEKKHVPELHLTAVRLKHDKTDADYLHVAREDKNNVFGVGFKTNPPDATGVPHILEHTTLCGSEKYPVRDPFFKMLPRSLSNFMNAFTSADHTTYPFATTNQQDFQNLLSVYLDATLHPLLKEEDFRQEGWRLGPEDPRASDALDGKPEDVLFKGVVYNEMKGQISDANYLYYIKYRESIFPALNNSGGDPQYITDLTHKQLVEFSKRNYHPSNAKFLTYGDMPLSTHLKQIGDVLDGFGKGEADTSVKLPIDLSRGPSNVTVPGPIDTFADADKQYKTSTSWYLGDTSEVVETFSAGILSSLLLDGYGSPMYRALIESGLGSSFTPNTGLDTSGRVPVLSVGLTGVSEEDAPKVKEAIQKVYQDSLSAGFSDEKVQGFLHQLELALRHKTANFGIGVMEKTISSWFNGVDPMKELAWNDVINEFKRRYQQGGYLESLMQKYLMNDRCLTFTMVGTPTFHQELDQQEMVRKEKKLSQLVEQHGSMEKAISSLREQELQLLKTQEEAQHADLGCLPSLRVEDISREKERKPVRESKVDDVDVVWREAPTNGLTYFQALNAFEDLPDDLRLLMPLFNDSVMRLGTANKTMEQWEDLIKLKTGGVSSSAFHTSSPTELGKFNEGLQFSGFALDKNIPDMLEILTTLITETDFTSPYAPAMIQELLRLTTNGALDSVAASGHRFALNAAAAGLSRSFWVQEQQSGLAQLQATANLLRDAETSPERLAELIEKLRL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Preferentially cleaves the N-terminal side of paired basic amino acid residues .2. The active site, Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "FKGRWTACVSTQVGCAMGCVFCATGQMGFTRHLAPGEIVGQILFVNRALAENGESLRNIVFMGMGEPLHNYDHTMTAIDILTDHKGLAIAPKHITLSTVGVVPGILRLAEEERPFRLAVSLHGATDEDRQKLVPPARRWPLAELMDACRTYTKARNRHIFFEWTLIEDENDTPEQAHALGQLLQTVDSHVNLIPLNPTLGYNGRPTQRPQVKQFQAILAEYGIPSTVRQRRGIS",
    "response#2": "LAVMNVGAPAAGMNAAVRAAVRIGLIHGHKMLAVHDGFEGLAHGQVEEIVWGGVGGWTGLGGSKLGTKRTLPKKYFEEISATISTFNIHGLIVIGGFEAFTGSLELVEGRSKYEELCIPLCVIPATVSNNVPGSDFSIGADTALNTITMTCDRIKQSAAGTKRRVFIIETMGGFCGYLATMAGLASGADAAYIYEDPFTIRDLQVNVEHLTEKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFESRKNVLGHMQQGGTPTPFDRNFISAEGKKGMYLPPL",
    "response#3": "YMAQRVVGTGSFGVVFQAKCLETGEQVAIKKVLQDKRYKNRELQIMRLQDHPNVVRLRHSFFSTTDKDELYLNLVLEFVPETVYRALKHYTKMNQHMPIILVQLYTYQICRALNYLHRVVGVCHRDIKPQNLLVNTHTHQLKICDFGSAKMLVPGEPNISYICSRYYRAPELIFGATEYTNAIDMWSGGCVMAELLLGQPLFPGESGIDQLVEIIKILGTPTREEIRCMNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALEACAHPFF"
  },
  {
    "reference": "MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAVRHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECI",
    "response#2": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECK",
    "response#3": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECR"
  },
  {
    "reference": "MHVLIAGAGLGGLTLAQNLRKRGISYEIFERDEHKDARFQGWAIALHTITDELVASMPSDMPDLRQATDHMSPLKIPTQICLYPAESNVRVGWEDCPETPFIRAERYRLRNHLATNIPIQWGKRVQRIEHDDQGVAVYFEDGTGAKGDILVGADGVKSVVRQQLLQKSHDEVLKIIPLAAIVGELKLSGEAFKRQLQLSHGSYSLIDSEKGYLTFCSLHDVAPDGKSARYYWIMSRSDPTIADPDHWLHKATQQEKLDHAREVMSNREPKFREIFDLTPVEGVKKETHIWRDLELSSLPAGRVVLMGDAAHVMTPFRGEGGYNTFIDAMALAKILDRLDKEDKTHDIESIKNSVNEYNAEMLERGVKSVQLSREWVDGSKVNSKKPLLAPMKVIPFSEVPLEVAA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a high affinity for FAD.2. The protein design must be able to perform monooxygenase activity, FAD binding in order to contribute to secondary metabolite biosynthetic process.3. The presence of FAD is necessary for the protein to function in the desired environment.4. The protein must exhibit the following characteristics: FAD-dependent monooxygenase; part of the gene cluster that produces the tetronate natural products trihazones; The pathway begins with the formation of trihazone A by the hybrid PKS- NRPS synthetase thnA and the trans-enoyl reductase thnE; The function of the FAD-dependent monooxygenase thnD has still to be identified.; Transcription analysis of thnD confirmed this gene is expressed, hence its role in the biosynthetic pathway remains cryptic. The designed protein sequence is ",
    "response#1": "VTYGDSIITQAGDSNQQAATNLKVGMRLPAAQVVRFCDAKVMKTVNALPSDGRWRIMIFPGDIRQPSASTRLAQLGTYLFSNHGPIRKYLPPGADIDSLIEVIVILSGERLEIQQDQIPDAFWPTTGKYRMRDLHKIYIDDETYHNGHGHAYDFYGIDPERGAVAIVRPDQYISKVLDMKNHEGISAFFEKFLQKA",
    "response#2": "KVLIIGGSVTGLTLAHSLDKMGVDYRLLEKRKEIAPQEGASIGILPNGARILDQLGLYNAIEQSAIALGTSDVYFPDGFHFTSSYPKRMHDRRSFAYPIAFMERRKLLEILYDLLPDKSRVEVDKAVSRIEEHPEHHGVLRAYTHDGDVYEGNLVVGADGVHSRTRREMWRLSGSSPTGDVPVSERNSTSVEYACIFGISDGIAELTPGRQVMRFGNGWTLAVIPSRQGQVFWFIVQKLDREYQYGSAPRFAPEDAAEQCSKLARLPIHGDVRFDDLWQRRKAVNMAALEENVFQTWSCGRLVCIGDSIHKMTVNLGQGANCAIEDVAVLCNILHHALNEKANSELSDVRASSLRNGVRAPNPRVCLATDYTAYLYDKMMGVGRFHLLLFGSDLQGPVRQRLAVLAGELRKREGFYERFGGREMFNLILVVKTLSHQTAELLEGDLAPLKDHATTVYDDRAPDDDAHYWYGVNHARGALVVVRPDLAVGVSVWPEEIGKLNKYFASFLLR",
    "response#3": "VTYGDSIITQAGDSNQQAATNLKVGMRLPAAQVVRFCDAKVMKTVNALPSDGRWRIMIFPGDIRQPSASTRLAQLGTYLFSNHGPIRKYLPPGADIDSLIEVIVILSGERLEIQQDQIPDAFWPTTGKYRMRDLHKIYIDDETYHNGHGHAYDFYGIDPERGAVAIVRPDQYISKVLDMKNHEGISAFFEKFLQKP"
  },
  {
    "reference": "MADQLTVIVLAAGGGTRMKSKTMKVLHRIGGRSMIGHVLGAVGGLEPTRVCAVVGHQREQVGPHILELLPEAVLAVQEQQLGTGHAVRVALEAMAADTGAPDSGTVLIAYGDTPLLEGATLRWFAAEHEAAQAAVSILSGVVEAPTGYGRIVRDDEGDVLAIVEEKDASAEQRMIREINSGILAFDAGFLLEAVGRIGNDNANGEYYLTDTVQLARDSGLTVTAFPVDDVWQTEGANDRAQLAVLGRELNRRIVTRWMKDGVSVMDPETTWIDADVVLAADVTILAGTQLLGATVVAEDAVIGPDTTLEDCEIGVGARVVRTHGQLAVIGDGATVGPFSYLRPGTLLGAGGKIGAFVETKNARIGDGAKVPHLSYVGDAEIGDGTNIGAGTIFANYDGVEKHRTVIGKHARTGSNNTFVAPVTVGDGAGTGAGTVVRKDVPPGALGLSSGQQRNLEGWAQRKRAGTAQGDAAAAATDEE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The presence of MobA-like NTP transferase domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MILAAGAGTRMRSATPKVLHRIAGRTLVAHALAAAAELVPGRTVVVVRHEREAVAGHIAEVAPDALIADQDDVPGTGAAVRCGLSTLDATTIAAAVAAGGADQAHLDSQVQGSIVVTSGDVPLVDGALLGSLVEAHEAQGNAVTVLTAQVPDATGYGRIVRDDAGAVTRIVEHKDATDAERAIGEINAGIYVFDAAHLRDALAREAGG",
    "response#2": "MAAGITLTAPETVFFTHDTALAADTVVEPHVVFGPGVTVTGPATIRAFSHLEGCEIAEGVTIGPYARIRPGSRIGKGARIGNFVETKNVEMGEGAKANHLTYLGDATVGAGSNIGAGTITCNYDGYIKSRTKIGDNAFIGSNSALVAPVSIGDGAIVGAGSVITKDVTGDAIAIARGEG",
    "response#3": "MAAGITLTAPETVFFTHDTALAADTVVEPHVVFGPGVTVTGPATIRAFSHLEGCEIAEGVTIGPYARIRPGSRIGKGARIGNFVETKNVEMGEGAKANHLTYLGDATVGAGSNIGAGTITCNYDGYIKSRTKIGDNAFIGSNSALVAPVSIGDGAIVGAGSVITKDVTGDAIAIARGEC"
  },
  {
    "reference": "MTLGIGVIGTGAIGQDHIRRVSRALSGGRIVALNDINADNARRAAQEWAPDAVICDTARDLVARPDVQAVMVTSWGGTHAEYVLDAIAAGKPVFCEKPLATNAADCLRIMEAEMARGRRLVQVGFNRRYDSGYLDLKAILDNGTIGDVLMVHAMHRNQRVGPNYKTEMAITDTLVHELDVHRWLLDGEYVSAQVIFPRRTSKALPHMRDPQIALLETKRGIRIDVEIFVNCQYGYDIQCAVVGELGQANLPDPPAVPVKTGETLSRHIMNDWKYRFIDAYDAEIQDFIDRASTGSPAGPDSWAGYAASVAADACVRAQESGRIEPIEMIAKPAFYSR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must have high specificity towards inositol 2-dehydrogenase activity. The designed protein sequence is ",
    "response#1": "VTWNVASAAPPLDLNDLLQLNNQNLNLDVYIIGLQETNCGIRSLLYDTAFEDPWSSFFMDVLSPLNFVKVSCVRMQGLLLLVFAKHQHLPFIQILSTKSTPTGLFGYWGNKGGINICLKLYGYYVSIINCHLPPHLANNDQRLEHFDRILEMQNFEEHDIPNILDHDLILWFGDMNFRIEDFGLHFVRESIKNRCYGELWEKDQLSIAKKRDPLLREFQEGPLLFPPTYKFDRNSSNYDTSEKKRKPAWTDRILWRLKRQPQASLHTPSQPVPHFSLSMRSYVSHMMYCISDHKPVV",
    "response#2": "VTWNVASAAPPLDLNDLLQLNNQNLNLDVYIIGLQETNCGIRSLLYDTAFEDPWSSFFMDVLSPLNFVKVSCVRMQGLLLLVFAKHQHLPFIQILSTKSTPTGLFGYWGNKGGINICLKLYGYYVSIINCHLPPHLANNDQRLEHFDRILEMQNFEEHDIPNILDHDLILWFGDMNFRIEDFGLHFVRESIKNRCYGELWEKDQLSIAKKRDPLLREFQEGPLLFPPTYKFDRNSSNYDTSEKKRKPAWTDRILWRLKRQPQASLHTPSQPVPHFSLSMRSYVSHMMYCISDHKPVFIMKPKSVSPDVPVSTIRNIKSEKGISGILVTEGGKYDGKLLGIVCTKDIDFVKDASAPC",
    "response#3": "VTWNVASAAPPLDLNDLLQLNNQNLNLDVYIIGLQETNCGIRSLLYDTAFEDPWSSFFMDVLSPLNFVKVSCVRMQGLLLLVFAKHQHLPFIQILSTKSTPTGLFGYWGNKGGINICLKLYGYYVSIINCHLPPHLANNDQRLEHFDRILEMQNFEEHDIPNILDHDLILWFGDMNFRIEDFGLHFVRESIKNRCYGELWEKDQLSIAKKRDPLLREFQEGPLLFPPTYKFDRNSSNYDTSEKKRKPAWTDRILWRLKRQPQASLHTPSQPVPHFSLSMRSYVSHMMYCISDHKPVV"
  },
  {
    "reference": "MDGQFEQKKKQKDETYDIEHLIACFSPMIRKKLSNTSYQEREDLEQELKIKMFEKADMLLCQDVPGFWEFILYMVDENS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Required for the acid stress-dependent induction of the oxalate decarboxylase oxdC.. The designed protein sequence is ",
    "response#1": "DNVAVIVPETDTQVTYRDLSHMVGHFQTMFTNPNSPLYGAVFRQDTVAISMRNGLEFIVAFLGATMDAKIGAPLNPNYKEKEFNFYLNDLKSKAICVPKGTTKLQSSEILKSASTFGCFIVELAFDATRFRVEYDIYSPEDNYKRVIYRSLNNAKFVNTNPVKFPGFARSSDVALILHTSGTTSTPKTVPLLHLNIVRSTLNIANTYKLTPLDRSYVVMPLFHVHGLIGVLLSTFRTQGSVVVPDGFHPKLFWDQFVKYNCNWFSCVPTISMIMLNMPKPNPFPHIRFIRSCSSALAPATFHKLEKEFNAPVLEAYAMTEASHQMTSNNLPPGKRKPGTVGQPQGVTVVILDDNDNVLPPGKVGEVSIRGENVTLGDNVAVIVPETDTQVTYRDLSHMVGHFQTMFTNPNSPLYGAVFRQDTVAISMRNGLEFIVAFLGATMDAKIGAPLNPNYKEKEFNFYLNDLKSKAICVPKGTTKLQSSEILKSASTFGCFIVELAFDATRFRVEYDIYSPEDNYKRVIYRSLNNAKFVNTNPVKFPGFARSSDVALILHTSGTTSTPKTVPLLHLNIVRSTLNIANTYKLTPLDRSYVVMPLFHVHGLIGVLLSTFRTQGSVVVPDGFHPKLFWDQFVKYNCNWFSCVPTISMIMLNMPKPNPFPHIRFIRSCSSALAPATFHKLEKEFNAPVLEAYAMTEASHQMTSNNLPPGKRKPGTVGQPQGVTVVILDDNDNVLPPGKVGEVSIRGENVTLG",
    "response#2": "VDMLKPRKRLDSGTWGVMGIGMGYCVAAAAVTGKPVIAVEGDSAFGFSGMELETICRYNLPVTVIIMNNGGIYKGNEADPQPGVISCTRLTRGRYDMMMEAFGGKGYVANTPAELKAALEEAVASGKPCLIF",
    "response#3": "IARAADLIKNAKRPVIMLGKGAAYAQCDDEIRALVEETGIPFLPMGMAKGLLPDNHPQSAAATRAFALAQCDVCVLIGARLNWLMQHGKGKTWGDELKKYVQIDIQANEMDSNQPIAAPVVGDIKSAVE"
  },
  {
    "reference": "MARSISNVKIVSAFVSRELSNAIFRRGYAATAAQGSVSSGGRSGAVASAVMKKKGVEESTQKISWVPDPKTGYYRPETGSNEIDAAELRAALLNNKQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in mitochondrion that is critical for response to hydrogen peroxide, regulation of photoperiodism, flowering, response to reactive oxygen species, response to light stimulus.2. The protein must exhibit the following characteristics: hydrogen peroxide H(2)O(2), diamide, menadione and tert-butyl hydroperoxide) by minimizing the negative effects of oxidation and monitoring photosynthesis during stress; Prevents premature aging (e.g; Promotes root development. The designed protein sequence is ",
    "response#1": "GVELVHAANKGLLKALFLVIPIKSKYAGVTYADIFQLASATAIEEAGGPKIPMIYGRADVADGEECPPEGRLPAADPPSPAEHLREVFYRMGLSDKEIVALSGAHTLGRARPERSGWGKPETKYTENGPGAPGGQSWTSEWLKFDNSYFKEIKERRDEDLLVLPTDAVLFEDSSFKIHAEKYAEDQDAFFEDYAEAHAKLSNLGAKFDPPKGISLC",
    "response#2": "GLVMNFYKDSCPQAEDIIKEQVKLLYKRHKNTAFSWLRNIFHDCAVQSCDASLLLDSTRRSLSEKETDRSFGLRNFRYIETIKEAVERECPGVVSCADILVLSAREGIVSLGGPYIPLKTGRRDGRRSRADVVEEYLPDHNETISGVLDRFAAMGIDTPGVVALLGAHSVGRTHCVKLVHRLYPEVDPALSPDHVPHMLHKCPDQIPDPKAVQYVRNDRGTPMVLDNNYYRNILDNKGLLIVDHQLAYDKRTRPYVKKMAKSQDYFFKEFSRAITLLSENNPLTGSKGEIRKQCR",
    "response#3": "GVELVHAANKGLLKALFLVIPIKSKYAGVTYADIFQLASATAIEEAGGPKIPMIYGRADVADGEECPPEGRLPAADPPSPAEHLREVFYRMGLSDKEIVALSGAHTLGRARPERSGWGKPETKYTENGPGAPGGQSWTSEWLKFDNSYFKEIKERRDEDLLVLPTDAVLFEDSSFKIHAEKYAEDQDAFFEDYAEAHAKLSNLGAKFDPPKGISLC"
  },
  {
    "reference": "MASRIINHSKKLKHVSALLRRDHAVAVRCFSNSTHPSLVGREDIFKARLNYSSVERISKCGTGNVTMLSGISTTSTKLSSPMAGPKLFKEFISSQMRSVRGFSSSSDLPPHQEIGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGFLAKIVKEEGAKEIQVGEVIAITVEDEDDIQKFKDYTPSSDTGPAAPEAKPAPSLPKEEKVEKPASAPEAKISKPSSAPSEDRIFASPLARKLAEDNNVPLSSIKGTGPEGRIVKADVEDFLASGSKETTAKPSKQVDSKVPALDYVDIPHTQIRKVTASRLAFSKQTIPHYYLTVDTCVDKMMGLRSQLNSFQEASGGKRISVNDLVIKAAALALRKVPQCNSSWTDEYIRQFKNVNINVAVQTENGLYVPVVKDADKKGLSTIGEEVRFLAQKAKENSLKPEDYEGGTFTVSNLGGPFGIKQFCAVINPPQAAILAIGSAEKRVVPGTGPDQYNVASYMSVTLSCDHRVIDGAIGAEWLKAFKGYIETPESMLL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein have Peripheral subunit-binding (PSBD), Lipoyl-binding domains with specific sequence motifs that are crucial for its activity.2. The protein should exhibit Basic and acidic residues compositional bias.3. The designed protein should be involved in glycolytic process, acetyl-CoA biosynthetic process from pyruvate by localizing to chloroplast envelope, cytoplasm, mitochondrion, mitochondrial matrix and exhibiting dihydrolipoyllysine-residue acetyltransferase activity, copper ion binding, acetyltransferase activity, lipoic acid binding.4. The design of the protein must focus on the precise arrangement of amino acids in the active site to enable effective catalysis of (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + acetyl-CoA = (R)-   N(6)-(S(8)-acetyldihydrolipoyl)-L-lysyl-[protein] + CoA.5. The protein must be engineered to have a high affinity for (R)-lipoate in order to increase its catalytic activity.6. For general function, the protein need meet that It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).; The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2) The designed protein sequence is ",
    "response#1": "TMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAITKKDAIITAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKESSFYGGHGIVGAQVPLGCGIAFAQKYNKEEAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTAEWRAAKSPSYYKRGDYVPGLKVDGMDAFAVKQACKFAKQHALEKGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIKKLVLSHDLATEKELKDMEKEIRKEVDDAIAKAKDCPMPEEMKAAAGKMVDVEVVKITRLRKAVSNAMTRSKSIIPETVLMDEINVDALVNFRNEAKGLAESKGIKLTYMAFIAKAVLIALKEFPMFNASFNHDTDEVYIKKFINLGMAVDTPDGLIVPNIKNADRLSVFELASQVRSLADDTIARKISMDQQTGGTFTITNFGSAGIAFGTPVINYPELAILGIGKIDRKPWVVGNEIKIAHTLPLSLAVDHRIIDGADGGRFLMRVKELLTNPTLLLG",
    "response#2": "RRMLMIRRFEERCGQLYGLGLIAGFCHLYIGQEAVAVGLQAALQPGRDSVITGYREHGHMLAYGIDPKIVMAELTGRASGISHGKGGSMHMFSTEHKFFGGNGIVGAQVPLGAGLAFAHKYRNDGGCSAAYFGDGSANQGQVYEAYNMAALWKLPVIFVIENNGYAMGTSIQRANAHTALSERGAGFGIPALVVDGMDVLEVRGAATVAVDWVQAGKGPIILEMKTYRYRGHSMSDPARYRSREEVNDMKENHDPLDNLKKDLFAAGVPEAELVKLDEDIRQQVKEAADFAEKAPLEFASPLARKLAEEKDLDLSQIRGSGPNGRIIKVDIENFQQSGAVVETTEKIPAMRKAIAKAMVNSKHTAPHVTLMDEIDVQELWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALNTSFNEEAGEVVHKHYWNIGIAADTDKGLLVPVVKHADRKSIFEISDEINELAVKARDGKLTSEEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLNG",
    "response#3": "TMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAITKKDAIITAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKESSFYGGHGIVGAQVPLGCGIAFAQKYNKEEAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTAEWRAAKSPSYYKRGDYVPGLKVDGMDAFAVKQACKFAKQHALEKGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIKKLVLSHDLATEKELKDMEKEIRKEVDDAIAKAKDCPMPEVVRELVLRSGETTEKIPAMRRAIAKAMVNSKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALNTSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDGKLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLE"
  },
  {
    "reference": "MLILDFRKYPFLKPLEDELKKYAGGISLNDLLVSGSYYLDQAKERIDKILKDKELESYDKIKDSVLVFYTTLYLVAALDNDMLKKKFLDKESQIIEKNLLNESEETLVEIANYLGLNINYNNIEIKYKKNKKTLSLSLKYSLNFIDFLKYTRELRKIDNKFSLASHILKDGKVYLTKEEIVRILVFQIRDKLMKMVNVSDVVIPEQIRKLADELKGRKTPPCILELKRKKELNNTELGVLIAYYIDIGDEKSVIDLTKDENIVKKFKGDKKTKYILYSCKKMKELGLCVASCNTLNPLQFYYGKLE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein requires [4Fe-4S] cluster to activate a critical catalytic site.2. The protein should have metal ion binding, 4 iron, 4 sulfur cluster binding and be active in primosome complex.3. For general function, the protein need meet that May also play a role in DNA repair.; Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication; The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis4. The designed protein should be able to compete with endogenous proteins or inhibitors for [4Fe-4S] cluster binding. The designed protein sequence is ",
    "response#1": "LSSQTKDGVTFAAMDRLKKHGLTIPSIFETSESVIEELIYPVGFWKKKAAFIKNATAICHDKFNNDIPNSLQGLLSLPGVGPKMAHICMNAAWGVVTGIGVDTHVHRIANRLKWVNTKKPEETRNCLEALLPRCEWDDINILLVGFGQR",
    "response#2": "DMREYDVPYHIRLSIDLKIHVAHWYNVRYRGNAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEAHLIQRWFEHVQETKPTIMVTYNGDFFDWPFVEARAAVHGLSMQQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVGSHNLKLSSQTKDGVTFAAMDRLKKHGLTIPSIFETSESVIEELIYPVGFWKKKAAFIKNATAICHDKFNNDIPNSLQGLLSLPGVGPKMAHICMNAAWGVVTGIGVDTHVHRIANRLKWVNTKKPEETRNCLEALLPRCEWDDINILLVGFGQP",
    "response#3": "GLNKPQKQAMKQVLLSRDYTLIVGMPGTGKTTTICALVRILSACGFSVLLTSFTHTAVDNILLKLAKFKVGFLRLGRAQKVHPDIRKFTEEEICRSKSIKSVTDLEELYNSQPVVATACMGINHPIFVQKQFDFCIVDEASQISQPICLGPLFCSKRFVLVGDHQQLPPLVQNSLLSSQTKDGVTFAAMDRLKKHGLTIPSIFETSESVIEELIYPVGFWKKKAAFIKNATAICHDKFNNDIPNSLQGLLSLPGVGPKMAHICMNAAWGVVTGIGVDTHVHRIANRLKWVNTKKPEETRNCLEALLPRCEWDDINILLVGFGQL"
  },
  {
    "reference": "MTENTHAGRLIVLTGPTAVGKGTVEAKLRADHPEVWVSVSATTRAPRPGEVDGVNYWFLTEDEFLAREAAGEFLETAVVHGMAHYGTLLKPVEEHLAAGVPTILEIDLQGARRVKQRAAELDLEVVYVFIAPPSFEELERRLIGRGTETAEQQARRLETAKVELAAESEFDVTIVNETVDQAAADLWSVIAKEYGL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have ATP binding, guanylate kinase activity within cytoplasm to regulate phosphorylation.2. The protein must exhibit the following characteristics: Essential for recycling GMP and indirectly, cGMP..3. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.4. The presence of Guanylate kinase-like domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MLVMSSPSGAGKTTISRALLERDPAIGMSVSATTRAPRPGEVDGKDYHFVTVEKFHEMVEKREFLEHARVFDNFYGTPRGPVDEILRSGRDVLFDIDWQGTQQMAQNARADLVSVFVLPPSVEELERRLRGRAQDSDEVVRKRMAKAGDEMSHWPEYDYIVVNIDLDKSIAAVQAILARY",
    "response#2": "MLVMSSPSGAGKTTISRALLERDPAIGMSVSATTRAPRPGEVDGKDYHFVTVEKFHEMVEKREFLEHARVFDNFYGTPRGPVDEILRSGRDVLFDIDWQGTQQMAQNARADLVSVFVLPPSVEELERRLRGRAQDSDEVVRKRMAKAGDEMSHWPEYDYIVVNIDLDKSIAAVQAILAQ",
    "response#3": "MLVMSSPSGAGKTTISRALLERDPAIGMSVSATTRAPRPGEVDGKDYHFVTVEKFHEMVEKREFLEHARVFDNFYGTPRGPVDEILRSGRDVLFDIDWQGTQQMAQNARADLVSVFVLPPSVEELERRLRGRAQDSDEVVRKRMAKAGDEMSHWPEYDYIVVNIDLDKSIAAVQAILAR"
  },
  {
    "reference": "MACGSVDPQGLLSPPLSSARLSNVPHVEGPWFWSCGQTNISCKVVNGKVVEVGKWPWQVSILFLGMYICSGSLIHHHWVLTAAHCLQRSKNPANYTVKVGVQTLPDNSSSELLVTSIVIHENFINHMSHDIAILKLKYPVTWSPFIQPICLPEVNFKPSIGTMCWVIGWGLEKAKGAPKTPYSVQGVAVRIVNNEICNHRYQFLLLKNQKKFIGNDMLCTSPEWGLDTCQDASGSSLVCQMNKTWIQMGVVSWNFGCGRRQFPSIYTSTSHFTQWIKRQIGDLKFASMAVPSFLSPFILTGYILLVSLGSLWLL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be optimized for serine-type endopeptidase activity.2. The designed protein must have a Charge relay system that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "IIGGFNCEKNSQPWHVAVYRFARYQCGGVLLDANWVLTAAHCYNDKYQVWLGKNNRFEDEPSAQHQLISKAIPHPGFNMSLLNKDHTPHPEDDYSNDLMLVRLKKPAEITDVVKPIDLPTEEPTVGSRCLASGWGSTTPTEEFEYSHDLQCVYLELLSNEVCAKAHTEKVTDTMLCAGEMDGGKDTCVGDSGGPLICDGVLQGITSWGPTPCALPNVPGIYTKLIEYRSWIKDVMN",
    "response#2": "IVGGKSAAFGRWPWQVSVRRTSFFGFSSTHRCGGALINENWIATAGHCVDDLLISQIRIRVGEYDFSHVQEQLPYIERGVAKKVVHPKYSFLTYEYDLALVKLEQPLEFAPHVSPICLPETDSLLIGMNATVTGWGRLSEGGTLPSVLQEVSVPIVSNDNCKSMFMRAGRQEFIPDIFLCAGYETGGQDSCQGDSGGPLQAKSQDGRFFLAGIISWGIGCAEANCV",
    "response#3": "VVGGEDARPNSWPWQVSLQYSSSGQWRHTCGGSLIEQNWVLTAAHCISSSRTYRVVVGRQSLSTVESGSLTIAVSKSVIHEKWNSNQLAQGNDIALLKLASSVPLTDKIQLGCLPAAGTILPNNYVCYVTGWGRLQSNGALPDILQQGKLLVVDYATCSNPSWWGSTVKTNMICAGGDGVTSSCNGDSGGPLNCQAANRQWQVHGIVSFGSSLGCNYYRKPSVFTRVSNYNDWISSVL"
  },
  {
    "reference": "MRFSALLVTLGLTGALATPTLVSREAPAVGVISDISAQTSALASAVSSYNGGDPSAVKSASEKLVSTINSGVDTVKSGPALSTADALALTSPVQDLTKQVEGVIDDLISKKDKFVAANAGGTVYEDLKAQYTAADSLAKAISAKVPESLSDIAAQLSAGITAAIQKGIDAYKDAASSTGTASSSAPATETATATETSTATGTVTETATSTPVIPTGTASGSASATPSTTATPTTGGSGSGSGSSTGTATASTSTNLLSTGAASKEHFSYSLGGAVVAAAIAVAL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be expressed specifically in the fungal-type cell wall2. The protein must exhibit the following characteristics: Antigen target of host humoral immune response..3. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "KTKNDIGLRLDISYILVNLIVCSYPVNTYPKLLYRNSLDDLILFLTVYHGKGNFRIFNFRGEKEDSDYKDNDLIGIAAKFESKDFEIQELRSTLINDGKIPISPIDLETRTLVEEETNNVICERIGWLDHFPPPFELLEEIVDGIENYLSVSKNRVAVLHCRMGKGRSGMITVAYLMKYLQCPLGEARLIFMQARFKYGMTNGVTIPSQLRYLRYHEFFITHK",
    "response#2": "KTKNDIGLRLDISYILVNLIVCSYPVNTYPKLLYRNSLDDLILFLTVYHGKGNFRIFNFRGEKEDSDYKDNDLIGIAAKFESKDFEIQELRSTLINDGKIPISPIDLETRTLVEEETNNVICERIGWLDHFPPPFELLEEIVDGIENYLSVSKNRVAVLHCRMGKGRSGMITVAYLMKYLQCPLGEARLIFMQARFKYGMTNGVTIPSQLRYLRYHEFFITHC",
    "response#3": "KTKNDIGLRLDISYILVNLIVCSYPVNTYPKLLYRNSLDDLILFLTVYHGKGNFRIFNFRGEKEDSDYKDNDLIGIAAKFESKDFEIQELRSTLINDGKIPISPIDLETRTLVEEETNNVICERIGWLDHFPPPFELLEEIVDGIENYLSVSKNRVAVLHCRMGKGRSGMITVAYLMKYLQCPLGEARLIFMQARFKYGMTNGVTIPSQLRYLRYHEFFITHR"
  },
  {
    "reference": "MLPVLTADALRTAEQAHWDEHPGDDLMGRAAAEVARHATEMLGDGPRPVVLVAAGPGSNAGDALHAMALLPPQLGAVVRIWPVRGRTHREGLDAALAAGAQVIGFEEAAELLARTDLLVDGIAGLGSRPLDGEAARLARAAHAQRVRVLSVDLPSGLRADAADAPEDAFRAERTVTFIAPKLCHVAAPAALLCGDVRRATLDVDPGETTTHQAAASDLPAWYPMPQASSQKYTRGLIGLETGSARYPGAAVLGAAGALFTGTGMVRHPGEAGAAVVSRFPSVVAAGGRVQAWTCGSGWDVEDPAARAAHLDRLAELIGEGRPMVLDAGALDLIAEVPPLPEGCLLTPHAGELARLLGREREDVEADPLGAAREAAARTGASVLLKGTVQAAADPDGRVVLAVRGPAWTAQAGSGDVLAGSAGALLAAGLPASRAGVLAASLQAVAATRSPGPIPPDRLATTFPEVIASWEDELVRTGDRSPDEQAPRGVRPS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a low Km value for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.2. The designed protein should have a binding site that is specific to the target (6S)-NADPHX ligand. The designed protein sequence is ",
    "response#1": "GPETVRSLFAPRAQNTNKGTYGTLLALCGSPGMAGAAVLSAAAALRCGAGLVNVALPKEIYPVAASYLPEPVYTLLETGRDGALTARSRKKLGDALLKATACLVGCGLGAGMEERALVRRLTGAVSVPLIVDADGINILARNIDILKTARVPVVLTPHPGEMARLIHSDARSVQSYRLRCARDFAREHGVVLVLKGSGTIVAAPDGRVFLNPTGNPGMAKGGSGDVLAGMIASFAAQGIDPVLAAVCGVYLHGLAGDRCAKRFSQRAMLPSDLVRELPALFSER",
    "response#2": "LVIGSSGNMPGAGIFAINSAMISGSGAGKIFVPKSLKQLIQSKMNSAIVSDYEDNNNGFLSVVNYNEAKKIVDWADAIAIGPGLGREKETAEFVYKLLEDYKGKRVVIDADAINALKNGGYQKFHLHNKVFTPHHKEFADLLGIELSELKQNLLNYGRKFTSETNSYLVLKGSTTIIFNPIGEIFINSSGNAGLAKFGSGDALTGLITSFIVQQESIEESIISAVYLHGLTADLIKKNESEFGIIPDKLINKLK",
    "response#3": "LVIGSSGNMPGAGIFAINSAMISGSGAGKIFVPKSLKQLIQSKMNSAIVSDYEDNNNGFLSVVNYNEAKKIVDWADAIAIGPGLGREKETAEFVYKLLEDYKGKRVVIDADAINALKNGGYQKFHLHNKVFTPHHKEFADLLGIELSELKQNLLNYGRKFTSETNSYLVLKGSTTIIFNPIGEIFINSSGNAGLAKFGSGDALTGLITSFIVQQESIEESIISAVYLHGLTADLIKKNESEFGIIPDKLINKLK"
  },
  {
    "reference": "MLCQNCNINEATIHLYTSVNGQKKQIDLCQNCYQIMKSGGQEALFGAGNASNGNSDEPFNPFNDIFSALQGQDFNGAASNQTPPTQTGGRGPRGPQNPRAKQPKGMLEEFGINITESARRGEIDPVIGRDEEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEGLAQKIVDGDVPQKLQNKEVIRLDVVSLVQGTGIRGQFEERMQKLMDEIRKRNDVIMFIDEIHEIVGAGSAGDGNMDAGNILKPALARGELQLVGATTLNEYRIIEKDAALERRMQPVKVDEPSVDETITILRGIQARYEDYHHVKYTDEAIEAAAHLSNRYIQDRFLPDKAIDLLDESGSKKNLTLKFVDPEDINRRIADAESKKNEATKAEDFEKAAHFRDQISKLRELQKQEVTDEDMPVITEKDIEQIVEQKTQIPVGDLKEKEQTQLINLADDLKAHVIGQDEAVDKISKAIRRSRVGLGKPNRPIGSFLFVGPTGVGKTELAKQLAKELFGSSESMIRFDMSEYMEKHSVAKLIGAPPGYVGYEEAGQLTERVRRNPYSLILLDEIEKAHPDVMHMFLQILEDGRLTDAQGRTVSFKDSLIIMTSNAGTGKVEASVGFGAAREGRTKSVLGQLGDFFSPEFMNRFDGIIEFSALSKENLLKIVDLMLDEVNEQIGRNDIHLSVTQAAKEKLVDLGYNPAMGARPLRRIIQENIEDSIADFYIEHPEYKQLVADLIDDKIVISNQTQETAETTDEEVPAE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.2. For general function, the protein need meet that Along with ClpP could be necessary for degrading proteins generated by certain types of stress.3. The protein must exhibit ATP hydrolysis activity, ATP binding, metal ion binding.4. The designed protein should contain one or more UVR domains that are essential for its function.5. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "GKANILLIGPTGTGKTLLAETLARFLDVPFAIADATTLTEAGYVGEDVENVLLRLLQNADFDVERAEMGIVYIDEIDKIARKSENPSLTRDVSGEGVQQALLKIIEGTVANVPPQGGRKHPHQEFIPVNTKNILFILGGAFEGLENIVKARVGKTTIGFTGGRREREEPLEVIPEDLIKFGMIPEFVGRAPLIVQLHPLTEDDLVRILTEPKNAR",
    "response#2": "AKSNILLIGPTGSGKTLLAETLARLLNVPFTIADATTLTEAGYVGEDVENIIQKLLQKCDYDVEKAQTGIVYIDEIDKISRKADNPSITRDVSGEGVQQALLKLIEGTIASVPPQGGRKHPQQEFLQVDTHNILFIVGGAFAGLDKVIRDRTEKGGIGFSAKVKSKEEKGPVSRTLSGVEPEDLIKYGLIPEFVGRLPVVATLEELDEDALIQILTEPKNAR",
    "response#3": "EKSNILLIGPTGSGKTLLAQTLARIIKVPFAIVDATALTEAGYVGEDVENIILKLIQNADNDIKKAEVGIIYIDEVDKIARKSDSASITRDVSGEGVQQALLKIIEGTVANVPPQGGRKHPHQEYLQVDTKNILFILGGAFVDLPNIIKSRTGIKTIGFGSEEQRIQSENKDVLMEQVIPEDLIKFGLIPEFIGRLPIL"
  },
  {
    "reference": "MVGALCGCWFRLGGARPLIPLGPTVVQTSMSRSQVALLGLSLLLMLLLYVGLPGPPEQTSCLWGDPNVTVLAGLTPGNSPIFYREVLPLNQAHRVEVVLLHGKAFNSHTWEQLGTLQLLSQRGYRAVALDLPGFGNSAPSKEASTEAGRAALLERALRDLEVQNAVLVSPSLSGHYALPFLMRGHHQLHGFVPIAPTSTQNYTQEQFWAVKTPTLILYGELDHILARESLRQLRHLPNHSVVKLRNAGHACYLHKPQDFHLVLLAFLDHLP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should contain a Helical; Signal-anchor for type II membrane protein membrane-spanning region.2. The protein should have hydrolase activity and be active in cytoplasm.3. For general function, the protein need meet that Possible role in granule neuron development. The designed protein sequence is ",
    "response#1": "ITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMRDKNPYSFQSDVYAFGILPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELL",
    "response#2": "PSVHKYNVSGANGTCLLASMGLQLNVTYSKRDNTTVSGLFSIDPSNTSATGSCGAQLVTLDLRSSRIHSLLFQFGMDPNTSQFFLQGIQLNMTVPDARDPTFQADNSSLRALQATIGNSYKCNAEERVEVTEAFSVNIFKVWVQAFQVC",
    "response#3": "PSVHKYNVSGANGTCLLASMGLQLNVTYSKRDNTTVSGLFSIDPSNTSATGSCGAQLVTLDLRSSRIHSLLFQFGMDPNTSQFFLQGIQLNMTVPDARDPTFQADNSSLRALQATIGNSYKCNAEERVEVTEAFSVNIFKVWVQAFQVC"
  },
  {
    "reference": "MSSPEISIKLKRLDHGAGLPLPAYATAHAAGLDVVSAEDVTLAPGARHAVATGFAIAIPEGYEVQVRPRSGLALKHGVTCLNTPGTIDADYRGEVKVILANLGSEPFEVKRGERIAQLVPAAVQRAAFAEVSDLDETARGAGGFGSTGR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA..2. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.3. The protein should be optimized for activity in dUMP biosynthesis, dUMP from dCTP (dUTP route): step 2/2, Pyrimidine metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The substrate binding site should be located in a region of the protein that is accessible to the ligand.5. The designed protein should have dUTP diphosphatase activity to facilitate dUTP catabolic process. The designed protein sequence is ",
    "response#1": "ELPEYTTPGAAGADLRANIEEPIALLPGQRALIPTGIKAEIPEGYELQVRPRSGLALKHGITVLNSPGTIDSDYRGEIRVILINFGDSTFIIEPKMRIAQVVLSPVVQATFVVKQESLAETARGSGGFGHTG",
    "response#2": "ELPEYTTPGAAGADLRANIEEPIALLPGQRALIPTGIKAEIPEGYELQVRPRSGLALKHGITVLNSPGTIDSDYRGEIRVILINFGDSTFIIEPKMRIAQVVLSPVVQATFVVKQESLAETARGSGGFGHTG",
    "response#3": "LLPVYATTHAAGMDVAACLEENVVVLPFETALIPSGFAIELPEGYEAQLRPRSGLALRHLISLPNTPATIDADYRGEVKIILINYGQEPFVVHHGDRVAQMVVAKVERVFFDEVLTLASSSRADGGFGHTG"
  },
  {
    "reference": "MSRKSIGEKRHSFSMRKLSVGLVSVTVSSFFLMSQGIQSVSADNMESPIHYKYMTEGKLTDEEKSLLVEALPQLAEESDDTYYLVYRSQQFLPNTGFNPTVGTFLFTAGLSLLVLLVSKRENGKKRLVHFLLLTSMGVQLLPASAFGLTSQILSAYNSQLSIGVGEHLPEPLKIEGYQYIGYIKTKKQDNTELSRTVDGKYSAQRDSQPNSTKTSDVVHSADLEWNQGQGKVSLQGEASGDDGLSEKSSIAADNLSSNDSFASQVEQNPDHKGESVVRPTVPEQGNPVSATTVQSAEEEVLATTNDRPEYKLPLETKGTQEPGHEGEAAVREDLPVYTKPLETKGTQGPGHEGEAAVREEEPAYTEPLATKGTQEPGHEGKATVREETLEYTEPVATKGTQEPEHEGEAAVEEELPALEVTTRNRTEIQNIPYTTEEIQDPTLLKNRRKIERQGQAGTRTIQYEDYIVNGNVVETKEVSRTEVAPVNEVVKVGTLVKVKPTVEITNLTKVENKKSITVSYNLIDTTSAYVSAKTQVFHGDKLVKEVDIENPAKEQVISGLDYYTPYTVKTHLTYNLGENNEENTETSTQDFQLEYKKIEIKDIDSVELYGKENDRYRRYLSLSEAPTDTAKYFVKVKSDRFKEMYLPVKSITENTDGTYKVTVAVDQLVEEGTDGYKDDYTFTVAKSKAEQPGVYTSFKQLVTAMQSNLSGVYTLASDMTADEVSLGDKQTSYLTGAFTGSLIGSDGTKSYAIYDLKKPLFDTLNGAT",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.2. The protein contains novel G5 domains that confer a unique function or activity.3. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation.4. The active site active site of the designed protein must be optimized for substrate binding and specificity.5. The designed protein should contain Extracellular topological domains.6. The designed protein should be involved in proteolysis by localizing to extracellular region and exhibiting zinc ion binding, metalloendopeptidase activity.7. The protein should exhibit Basic and acidic residues compositional bias.8. The protein must exhibit the following characteristics: Zinc metalloproteinase that specifically cleaves human matrix metalloproteinase 9 (MMP-9), leading to its activation. The designed protein sequence is ",
    "response#1": "GRPVWNKRYITYRINNYTPDMRREEVDYAIEKAFRVWSNVTPLKFRKINTEEADIMILFAHGAHGDYGAFDGKGGVIAHAFGPGDGIGGDTHFDEAEIWTQNSRGTNLFLVAVHEFGHALGLSHSNDPTAIMFPTYGYLDLNTFRLSADDIRGIQSLYGD",
    "response#2": "VAVAAEKDKWPNGRVPYILSAAYTSAQRAVLARAFDTYAKRTCIRFVPKSPADKDYIVIQKLDGCYADFSRVGGRQQVSLADECIDYATIIHELMHVIGFIHEHQREDRDSYVSILYQNVIQGANTDFDKLSNLGLSYYGEHYDYSSIMHYEANEGSRNGKNTIEAKNSHFTAIMGKASDFSTSDLRRVNRAYKCS",
    "response#3": "VAVAAEKDKWPNGRVPYILSAAYTSAQRAVLARAFDTYAKRTCIRFVPKSPADKDYIVIQKLDGCYADFSRVGGRQQVSLADECIDYATIIHELMHVIGFIHEHQREDRDSYVSILYQNVIQGANTDFDKLSNLGLSYYGEHYDYSSIMHYEANEGSRNGKNTIEAKNSHFTAIMGKASDFSTSDLRRVNRAYKCK"
  },
  {
    "reference": "MGGKWSKRNIIGWSTVRERLRRAEPAADGVGPASRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLDGLTYSQQRRDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEQEEVEAANEGENNSLLHPMSQHGMDDPEKEVLKWQFDSRLAFTHMAREMHPEYYKNC",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should contain at least one PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Dileucine internalization motif; necessary for CD4 internalization motif to achieve its intended function.2. A protein that contributes to suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host autophagy by functioning in extracellular region, host cell Golgi membrane, membrane, host cell plasma membrane, virion component with SH3 domain binding.3. For general function, the protein need meet that Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPAVRERIRRTDPAAEGVGAVSRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPREVEEANEGENNCLLHPISQHGMEDEDREVLKWKFDSALARRHMARELHPEYYKDC",
    "response#2": "MGGKWSKSSIVGWPEIRERIRRAEPAAEGVGAVSRDLEKHGAITSSNINHPSCVWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLVYSQKRQDILDLWVYNTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPEKVEEANEGENNSLLHPISLHGMEDPEKEVLMWKFDSRLAFHHMARELHPEYYKNC",
    "response#3": "MGGKWSKSSIVGWPQVRERIRQTPPAAEGVGAVSRDLDKHGAVTSSNINHPSCVWLEAQEDEEVGFPVKPQVPLRPMTFKGALDLSHFLKEKGGLEGLIYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPGEVEEDNKGENNSLLHPISQHGMDDPEKEVLMWKFDSRLAFHHVARELHPEYFKDC"
  },
  {
    "reference": "MELMLIQGSQMHQLLMHGLAIAALMPLGAGTAPAQALAGLSQGEEEEEEEAVVFHHHYLPWTLPRLPPVRFLRSSPEEGRLLRAALLPLKRFYGIRMRVRGSERLRLPGPFVIVCNHQASLDLMGMVEVIPERCVPIAKRELLYMGTVGWACWLSGIIFIERQRRDAAIEVITRTASTMRRENLRVWVFPEGTRNPGRSMLPFKRGAFHLAVHAQVPIVPIVISPYRDFFSPEEKRFTSGTCTIQVLPRVETCGLSPKDVPKLTESVQQAMAEALAEMSMSDHREQDMEQPLLGPGEDRDSPQGQGDAARSSK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.2. The designed protein should have a specific function within the membrane.3. Include a signal peptide as an essential component of the protein.4. The protein must exhibit the following characteristics: Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.. The designed protein sequence is ",
    "response#1": "LVLMLNHQTSLDIMVMAEMIPSHCVPIAKKEILYMGPFGLACWLSGFAFIDRKKKEESIAALTVVAESMHRENFGIVIFPEGTRNHCGSMLPFKRGGFHLAVKAQVPIVPAVISSF",
    "response#2": "CVIISNHQSILDMMGLMEALPKRCVQIAKRELLFMGPVGLIMYLGGVFFINRQRSRTAMTVMADVGERMVRENLKVWVYPEGTRNDNGDLLPFKKGAFYLAIQAQVPIIPVVYSSE",
    "response#3": "MQVWGTEHLDLEEPYVIVCNHQASLDLLGMVEVIPERCVPIAKKELLYMGTVGWACWLSGIIFIDRHRREAAIDVISQTARTMWRENLRVWVFPEGTRNQNKSMLPFKRGAFHLAVQAQVPIVPIVIFDIKGRQKLEVDHPCVIISNHQSILDMMGLMEALPKRCVQIAKRELLFMGPVGLIMYLGGVFFINRQRSRTAMTVMADVGERMVRENLKVWVYPEGTRNDNGDLLPFKKGAFYLAIQAQVPIIPVVYF"
  },
  {
    "reference": "MGGKWSKRNMDGWSIVRKKMEQAEPAAEGVGAVSRDLEKHGAITSSNTASTNAACAWLEAQEDEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLVYSQKRREILDLWVYHTQGFFPDWQCYTPGPGIRYPLTFGWCFKLVPVDPEQVEKANEGENNCLLHPVSQHGMDDTEREVLIWRFDSRLAFHHVARDQHPEYYKDC",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should contain at least one Dileucine internalization motif; necessary for CD4 internalization motif to achieve its intended function.2. For general function, the protein need meet that Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis3. The designed protein should have SH3 domain binding within virion component to regulate suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host autophagy. The designed protein sequence is ",
    "response#1": "MGGKWSKRSKPGWPAVRERMRRTEPAAEGVGAASQDLDKYGALTSSNTPATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLVYSQKRQDILDLWVYNTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVEPEKVEEANEGENNCLLHPMSLHGMEDPEKEVLVWKFDSRLAFHHMARELHPEYYKDC",
    "response#2": "MGGKWSKRSGAGWSTVRERMRRAEPAAEGVGAASRDLEKHGALTSSNTPATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVEPEKVEEANEGENNCLLHPMSLHGMEDPEKEVLEWRFDSRLAFHHMARELHPEYYKDC",
    "response#3": "MGGKWSKSSLVGWPAIRERMRRAEPAAEGVGAASRDLERHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLREKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRFPITFGWCFKLVPVEPEKIEEANEGENNCLLHPMSQHGMDDPEREVLMWKFDSRLAFHHVARELHPEYYKN"
  },
  {
    "reference": "MQKNKSRKVDLKDLTQDQLVRFVESLGQPAFRGRQILAWIYRPGISEFSQMTDLAKEFRAVLSQAATMSRFDDYLVESSMDGTIKFGFRLKDGLVIESVLIPEEERNTLCVSSQVGCAMGCSFCLTGKMGFHRNLRTAEIVNQVCAAQEWSFSHERGPLTNLVFMGMGEPLANLESVLDAISILTEQRGLDFSNRRITVSTCGLVPQMQILGEQTGVNLAISLHAVDDEVRSSLMPINRRYPLQQLIEASRTYPQKRRKRVMFEYTLLEGVNDADTDAEKLADLLHNVPCKINLLSVNPTGDARFHSPKEERILEFQKILRDRGFTVFIRQSRGEDIAAACGQLAGKGWPVNQDSESQLK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The ideal protein would be one that plays a critical role in rRNA base methylation through 4 iron, 4 sulfur cluster binding, metal ion binding, rRNA binding, tRNA binding, rRNA (adenine-C2-)-methyltransferase activity within cytoplasm.2. For general function, the protein need meet that m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs3. The presence of Radical SAM core domains is critical for the interaction of the designed protein with its intended targets.4. The designed protein should have a high specificity for S-adenosyl-L-methionine binding, with minimal off-target effects.5. The S-methylcysteine intermediate, Proton acceptor of the protein must be engineered to enable effective catalysis of adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "MTYGELAAFVTGELGEPAYRAEQIFRWLHGRGVTSVDEMTNVPKPLRARLAERAHVDLLGLNKMQIQGALMESQLDVKQAKLRAKQLFHWIYHRGETDFAMMTDLAKPMRLWMADRFVISDTPTNLYDLTRPALGALLSDWGFRPHHRDVLWTGLYRQQVRALDEVEGLRSDLRDTLRERTRLSHLATHHEAFSSDGHTHKLLLRLEDGQTIETVLMRFKGRATVCISTQAGCAMGCVFCATGQMGLSRHLTPGEIVGQILHVQRILRETGESLRNVVLMGMGEPLHNYDNTLAAVDVLVDPLGLAMGPRFITLSTVGVVPGIRRLADEDRPVQLAVSLHGATDAERAALVPAGRRWPLAELMDACRYYIARRKRRIFFEWTLIAGRNDTSEHAHTLGALLGGMDAHVNVIPLNPTVGYDGDPSQPASVRAFQDVLMSHGVPSTVRQRRGIDIDAGCGQLKSAVERRSRLTRG",
    "response#2": "MTYGELAAFVTGELGEPAYRAEQIFRWLHGRGVTSVDEMTNVPKPLRARLAERAHITSDTPTNLYDLTRPALGALLSDWGFRPHHRDVLWTGLYRQQVRALDEVEGLRSDLRDTLRERTRLSHLATHHEAFSSDGHTHKLLLRLEDGQTIETVLMRFKGRATVCISTQAGCAMGCVFCATGQMGLSRHLTPGEIVGQILHVQRILRETGESLRNVVLMGMGEPLHNYDNTLAAVDVLVDPLGLAMGPRFITLSTVGVVPGIRRLADEDRPVQLAVSLHGATDAERAALVPAGRRWPLAELMDACRYYIARRKRRIFFEWTLIAGRNDTSEHAHTLGALLGGMDAHVNVIPLNPTVGYDGDPSQPASVRAFQDVLMSHGVPSTVRQRRGIDIDAGCGQLKSAVERRSRLAAAA",
    "response#3": "MTYGELAAFVTGELGEPAYRAEQIFRWLHGRGVTSVDEMTNVPKPLRARLAERAHFASDTPTNLYDLTRPALGALLSDWGFRPHHRDVLWTGLYRQQVRALDEVEGLRSDLRDTLRERTRLSHLATHHEAFSSDGHTHKLLLRLEDGQTIETVLMRFKGRATVCISTQAGCAMGCVFCATGQMGLSRHLTPGEIVGQILHVQRILRETGESLRNVVLMGMGEPLHNYDNTLAAVDVLVDPLGLAMGPRFITLSTVGVVPGIRRLADEDRPVQLAVSLHGATDAERAALVPAGRRWPLAELMDACRYYIARRKRRIFFEWTLIAGRNDTSEHAHTLGALLGGMDAHVNVIPLNPTVGYDGDPSQPASVRAFQDVLMSHGVPSTVRQRRGIDIDAGCGQLKSAVERRSRRTPPRPASLRSDTPTNLYDLTRPALGALLSDWGFRPHHRDVLWTGLYRQQVRALDEVEGLRSDLRDTLRERTRLSHLATHHEAFSSDGHTHKLLLRLEDGQTIETVLMRFKGRATVCISTQAGCAMGCVFCATGQMGLSRHLTPGEIVGQILHVQRILRETGESLRNVVLMGMGEPLHNYDNTLAAVDVLVDPLGLAMGPRFITLSTVGVVPGIRRLADEDRPVQLAVSLHGATDAERAALVPAGRRWPLAELMDACRYYIARRKRRIFFEWTLIAGRNDTSEHAHTLGALLGGMDAHVNVIPLNPTVGYDGDPSQPASVRAFQDVLMSHGVPSTVRQRRGIDIDAGCGQLKSAVERRSR"
  },
  {
    "reference": "MGSFSLLHWLVVLVIVLLVFGTKRLANGAKDIGSAIKEFKKSLHEDDKPTDQLGSTSQSTASGPQQDHGKH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that TatA could form the protein-conducting channel of the Tat system.2. The designed protein must be able to regulate protein transport by the Tat complex. The designed protein sequence is ",
    "response#1": "GHTARKRFGQNFLHD",
    "response#2": "MVSPMSASS",
    "response#3": "MPFVKNN"
  },
  {
    "reference": "MGYVVSTFYRFVHLSNYYDIRPVLKEFCVQNNIKGTIILAEQGINATIAGSQNSLDKFFSFLDLDSRLCNLQYHKSFSRCNPFSKMKVKLRKELVCLGIEDFDDSVGGEYIDPENWDNFISRSDVYTIDTRNSYEINFGKFKNSINPETNCFRDFPDWAISWAKNKIDDDPIIAMYCTGGIRCEKSTAFMKDLGFSKVYHLKGGILEYFKSTGNINNLWEGYCFTFDDRIIVDDKLVPGDVKCILCGAHVMLEDMKSISRGHVLCFSCKDHV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A key design requirement for the protein is the optimization of the Cysteine persulfide intermediate to achieve high catalytic efficiency for AH2 + O2 + uridine(34) in tRNA = 5-hydroxyuridine(34) in tRNA   + A + H2O.2. The protein must exhibit the following characteristics: Catalyzes oxygen-dependent 5-hydroxyuridine (ho5U) modification at position 34 in tRNAs..3. The protein design must be able to perform oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen in order to contribute to tRNA modification. The designed protein sequence is ",
    "response#1": "PEDWNQLLADPDVVVIDVRNDYEVAIGTFKGAINPNTKSFSELPEWLQEQAELQKKPKVAMFCTGGIRCEKSTALLRHEGFEDVFHLQGGILSYADENTVVVDKRNDYEYAIGTFEGAIDPQTRTFREFPEWVKQNRDRLEGKKIAMFCTGGIRCEKATAFVKGLGFDDVYHLKGGILKYLEEVPREQD",
    "response#2": "VEPEDWNDLIRSDDVVLIDTRNDYEVAIGTFEGAIDPMTESFRDFPAWWEANKERFHNKRVAMFCTGGIRCEKSTNYLLGQGVEDVYHLKGGILRYLEEVPQ",
    "response#3": "LSPSEFREALEDDDTIVIDARNDYEFDLGHFRGAVRPNITRFRDLPDWIKENKEVFMDKKIVTYCTGGIRCEKFSGFLLKEGFEDVAQLEGGIATYGKDPEK"
  },
  {
    "reference": "MQELEEVPAKYLDKFIETHLLPDTHFRRQIKEAVHIISTFLKERCFQGAPHPVRVSKVVKGGSSGKGTSLRGRSDADLVVFLNNLGSFQDQLDNRGTFIREIKRQLEACQREETFEVEFEVGSLQWEKPRALSFTLMHPHLHEGVEFDVLPAFDVLGQWTNSGRPNPRVYMELIEECQDLGREGEFSPCFTELQRDFLRQRPTKVKSLIRLVKHWYQKCKQQLGKPLPQQYALELLAVYAWERGSGQTEFSTAQGFRTVLQLVLNYQQLCIYWTKYYNFEHPIIGQYLRMQLAKPRPVILDPADPTGNVAGGDSHSWWRLAEEARDWLHYPCFQKGDGSPVGSWNVPLEEDSEDDWFTCEHRVPQFQGTGWHPVPAGRSLSTGMTPATAQREDDWMCAIL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should contain one or more 2'-5'-oligoadenylate synthetase, Polymerase nucleotidyl transferase domains that are essential for its function.2. The desired protein should be able to perform 3 ATP = 5'-triphosphoadenylyl-(2'->5')-adenylyl-(2'->5')-   adenosine + 2 diphosphate3. The ideal protein would be one that plays a critical role in positive regulation of monocyte chemotactic protein-1 production, regulation of ribonuclease activity, glucose metabolic process through double-stranded RNA binding within nucleoplasm. The designed protein sequence is ",
    "response#1": "KPKPQVYSTLLNSGCQAGEHAACFAELRRNFVNTRPVKLKNLILLVKHWYRQVCPAMAAGKETLPPPYALELLTIFAWEQGCGKDAFSLARGLRTVLGLIQQYQHLCVFWTVNYGFEDPAVARFLRAQLERPRPVILDPSDPTWDVGNGAAWHWARLAQEAESCYDQPCFLQESGVPVQPWH",
    "response#2": "KPKPQVYSTLLNSGCQAGEHAACFAELRRNFVNTRPVKLKNLILLVKHWYRQVCPAMAAGKETLPPPYALELLTIFAWEQGCGKDAFSLARGLRTVLGLIQQYQHLCVFWTVNYGFEDPAVARFLRAQLERPRPVILDPSDPTWDVGNGAAWHWARLAQEAESCYDQPCFLQESGVPVQPWR",
    "response#3": "KPKPQVYSTLLNSGCQAGEHAACFAELRRNFVNTRPVKLKNLILLVKHWYRQVCPAMAAGKETLPPPYALELLTIFAWEQGCGKDAFSLARGLRTVLGLIQQYQHLCVFWTVNYGFEDPAVARFLRAQLERPRPVILDPSDPTWDVGNGAAWHWARLAQEAESCYDQPCFLQESGVPVQPWR"
  },
  {
    "reference": "MNVILSVMLFSSPSCVNINSFDILIVGAGISGIVLANILANHNKRVLIVEKRDHIGGNCYDKVDSKTQLLFHQYGPHIFHTNNQTVINFISPFFELNNYHHRVGLKLKNNLDLTLPFDFQQIYKLMGKDGRKLVSFFKENFSLNTHLSLAELQLIDNPLAQKLYQFLISNVYKPYSVKMWGLPFAMINENVINRVKIVLSEQSSYFPDAIIQGLPKSGYTNSFLKMLANPLIDVQLNCKDNLLVYQDEKLFFNNNLIEKPVVYCGLIDKLFNFCFGHLQYRSLAFSWKRFNQKKYQTYPVVNMPLAKSITRSVEYKQLTNQGSFKPQTIVSFETPGSYAINDPRFNEPYYPINNTLNDTLFKKYWKKASKLKNLHLLGRLATYQYIDMDKAILLSIKKAQQLLS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit flavin adenine dinucleotide binding. The designed protein sequence is ",
    "response#1": "ATGTFDIIHPGHGFFLEEARKLGGEDARLVVVLARDSTVRARKRTPIVGEKQRLEVVRMLKPVDEAYLGSETDMFEIVHRIKPDIIAIGPDQKFDVDELRDELRRRGLGCEVRRIEKYRDAELDSTCKIIKG",
    "response#2": "ATGTFDIIHPGHGFFLEEARKLGGEDARLVVVLARDSTVRARKRTPIVGEKQRLEVVRMLKPVDEAYLGSETDMFEIVHRIKPDIIAIGPDQKFDVDELRDELRRRGLGCEVRRIEKYRDAELDSTCKIIKK",
    "response#3": "ATGTFDIIHPGHGFFLEEARKLGGEDARLVVVLARDSTVRARKRTPIVGEKQRLEVVRMLKPVDEAYLGSETDMFEIVHRIKPDIIAIGPDQKFDVDELRDELRRRGLGCEVRRIEKYRDAELDSTCKIIK"
  },
  {
    "reference": "MAELSTVARPYAEALFSAARDDKAGLPAWADLIGELAQVAGNPDVREAMTDPRLGDAQRIELFTGLVKTQLPQAVRNFIELLVANDRLLLLPVIAKQFVALKNRHEGTAQAEITSAFELSDAQVKELIEALETKFGLKLKPSVTVDKSLIGGVRVAVGDQVLDTSVQAQLARLRDTLAA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a specific function within the plasma membrane. The designed protein sequence is ",
    "response#1": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPR",
    "response#2": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPA",
    "response#3": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPS"
  },
  {
    "reference": "MTTVTSRTSRQMMNSHVRNGNGIKLTLAQRKRACYCEGDNLAHYHCTKCDIICDSLTKARPVNHDCDEEPFITYDDDISLPIHTTTLKDFLNESDFGHITNENAIHQEELSNNIEVIRVSQPKFDVVEVTKMEINDLEGKRGLNTTIKDDGEYDAKIIGETLPKGDQTGDNTYQFIQFGSFEPVAVRARTDEKHPIIEKKPLLKQSVKEMHSDPSKEAKSISTGWNRRVKGAKNPQTGAQQMKPRIDTTCNNRKKVWVKVVKPTLEEPRVVETIPETKVVESRPIFSGSLYKRKERCKSKTSQDEKIVITASTLCDEIIHLSYARNLPLIVIGKKKHEFRTVDLNGKRYYKILTKHERGIVDQLDVNNNAETISLIRLFLSKNPDDLVDEFDIRKGHSGLIINPDNIIGRRPLTHKKDVMVVRGRLHGRIVDSLIPIHAHKIGDIEHYASDTTTASEIFKGFEKTFTAIRDPVQHICVRDISLQECGEMCGVLVQMMFPMWKITCSQCASLIEGRNQDQILTDASRTKVIEMYEQMVSTGKFKHVQTVINSLKTFEDITQESLKLFGDIDSASLNRATSQFSQINVIAQILVKGQAMKPDEQELALQNLKDLMLWYRHKLEAQQTGDISTFRNKVSAKTHINLALMCDNQLDVNGIFQWGERGYHAKRFFEKYFTRIDSGDQYEAFTTRRHVRGSRQLAIKNLIVTTDIERMTQSMQGLPVANIPLGEYCVSRFDKNFVYPCCCVTHDNGKIMHSEFKFPTKNHLVIG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification..2. Include a signal peptide as an essential component of the protein.3. The designed protein's functionality will benefit from Polar residues, Basic and acidic residues compositional bias in its structure.4. The For helper component proteinase activity of the protein must be engineered to enable effective catalysis of Hydrolyzes glutaminyl bonds, and activity is further   restricted by preferences for the amino acids in P6 - P1' that vary   with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or   Gly) for the enzyme from tobacco etch virus. The natural substrate is   the viral polyprotein, but other proteins and oligopeptides   containing the appropriate consensus sequence are also cleaved..5. The designed protein have RdRp catalytic, Helicase ATP-binding, Peptidase C6, Helicase C-terminal, Peptidase C4 domains with specific sequence motifs that are crucial for its activity.6. A protein that contributes to RNA-protein covalent cross-linking by functioning in helical viral capsid, host cell cytoplasmic vesicle with structural molecule activity, hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides, helicase activity. The designed protein sequence is ",
    "response#1": "QQNSALQRKLLIQRQSPVKQTQKGAVQLRHVQYEVAVERERMTEKRRLEEEKFLAGDYSNDTYIGSVPYIRGAQKTGETVSFRSPHYRRTAKAPNRVKKGKQYCNQQHVLSSLLRICSKTGVTIEIVGKKRTNSLKVRYVKKYGCTLPQARLPHHDGVYKHTEVQMAPYERFLTAMCDISKYRAVEASSIQRGDSGLVFDRTVAITREHTKLPFFIVRGRSNGKLINALEKYHQYHSIEKHNENNISELIHKLTRIVHDRQLDVHFVGKRRLDGRCVRSDGKSYLQLQTKHTLGVQSGVDCYIPKGLEHLVHAFAKGSGYKSRIQVSKIKKGWSGFILQPTKIIGQVGKCKHDFLVVRGSLRGELIDACTRVIPSSELEHQVLIHTPITNAETQTTQHTTPDPSFVKPQSRQRTTHVTSGVSGQHFSELIRNLAQIVHDRQLDVHFVGKRRLDGRCVRSDGKSYLQLQTKHALGLKNRVDCHIPKGLEHIVHELAKGSGYKSRTRVRDIKKGWSGFVLQPRNIIGLSGKSKHDFFVVRGSLKGKLIDNASDRKDGNNVSELIHRLTQIVHDRQLDVHFVGKRRLDGRCVQIDGKSYLQVQTKHTRGYKRKVDCHIPRGLEHIIHMLAKGNGYKSRTRIGNIRKGWSGCILQPENIIGRVGKRMHDFFVVRGVSNGELIDQVLIHTPITNAETQTTQHTTPDPSFVKPQSRQRTTHVTSGVSGQHFSELIRNLAQIVHDRQLDVHFVGKRRLDGRCVRSDGKSYLQLQTKHALGLKNRVDCHIPKGLEHIVHELAKGSGYKSRTRVRDIKKGWSGFVLQPRNIIGLSGKSKHDFFVVRGSLKGKLID",
    "response#2": "VMTDSQFNWFVKQSKNIMTEKRGCIELVGQKTTKLYYKSTRFGVRCTCELAHVRGERKRVDAVIPRDHVPLAKVLARVPTWGRAIHSEELRRGDSGMILRSESCKGTFGRSWDEMFIVRGAYEGKILDARSKLTETTVLRMVQKKQCGSSTLVLREVLSLAAKSGMPIEFITKRKGKDFKVSYVRKHGAMLPKFILPHEAGKYVRQELQYANISEFLPHICKFAKYRAIRANDITHGDSGLLFDKRSSITVEPTELPYFVVRGRKHGKLVNAFDAFKDIEDIEHIHVKANLNELIRQTLDVAIANKLPIEYIDKRVKFSKKAHPTLLSVPVCHAYDPELGCKDDINDDVLRNILNHAQFEKLISNEMNCRFNSKALQPGWSGVVLTSRMVEDQDQFEFSSNGICVVKGRGTNGKILNALWAHDSTEIAEFYSGVLPSLTKNVTITSESLCDELLNIVRERKLSLTIISKSKHEFNSVELNGKMYYKVVTNHENGVINCLDMNNNEETISLLRFFQARNPDDLIDEFDIRKGHSGLIINPDNIIGRRPLTYKEDVMVVRGRLYGRVVDSLLKIRRNKVCDIEHELEVTLQPYFDRRQFANVRKKANGTMVYKRPTANKVSKLRKRALTLKKEQEDFVLGAPSIVSTISIAGGVAPSQLQEESKAVKPLHTTPSRKRKTSHRKVVMTDSQFNWFVKQSKNIMTEKRGCIELVGQKTTKLYYKSTRFGVRCTCELAHVRGERKRVDAVIPRDHVPLAKVLARVPTWGRAIHSEELRRGDSGMILRSESCKGTFGRSWDEMFIVRGAYEGKILDARSKLTETTVLRMV",
    "response#3": "VMTDSQFNWFVKQSKNIMTEKRGCIELVGQKTTKLYYKSTRFGVRCTCELAHVRGERKRVDAVIPRDHVPLAKVLARVPTWGRAIHSEELRRGDSGMILRSESCKGTFGRSWDEMFIVRGAYEGKILDARSKLTETTVLRMVQQVLIHTPITNAETQTTQHTTPDPSFVKPQSRQRTTHVTSGVSGQHFSELIRNLAQIVHDRQLDVHFVGKRRLDGRCVRSDGKSYLQLQTKHALGLKNRVDCHIPKGLEHIVHELAKGSGYKSRTRVRDIKKGWSGFVLQPRNIIGLSGKSKHDFFVVRGSLKGKLIDPKDPLKHNENNISELIHKLTRIVHDRQLDVHFVGKRRLDGRCVRSDGKSYLQLQTKHTLGVQSGVDCYIPKGLEHLVHAFAKGSGYKSRIQVSKIKKGWSGFILQPTKIIGQVGKCKHDFLVVRGSLRGELIDACTRVIPSSELEHELEVTLQPYFDRRQFANVRKKANGTMVYKRPTANKVSKLRKRALTLKKEQEDFVLGAPSIVSTISIAGGVAPSQLQEESKAVKPLHTTPSRKRKTSHRKVVMTDSQFNWFVKQSKNIMTEKRGCIELVGQKTTKLYYKSTRFGVRCTCELAHVRGERKRVDAVIPRDHVPLAKVLARVPTWGRAIHSEELRRGDSGMILRSESCKGTFGRSWDEMFIVRGAYEGKILDARSKLTETTVLRMVPAEDPYWLPLHKMYIAKAGYCYMNIFLAMLVNVRKEEAKAFTKMVRDVLINQLGTWPTLLDVASACYLLKVFFPDVSGAELPRIMIDHKTQTMHVIDSYGSLNTGYHILKANTVEQLIKFTRAGLKSDMKHYLV"
  },
  {
    "reference": "MKFNFITLFPDKIQSYFSEGLQQKAIESGVFSVNIIQLRNFSGNKHNRVDDTIYGGGPGMLLRVEPIHKAILSLGEEKGIVILTSPSGIPFNQSIAMDLKKGGKPLTFISGYYEGVDHRVTEHLVDMEMSLGNYVLSAGDLASICIADAVSRLLPGFLGADESLLDESHNHPDILEYPQFTKPSEYNGWKVPDVLLSGNHASILAWREQNRKKIERGNYESTFKRSADSGC",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be able to bind S-adenosyl-L-methionine ligand in a variety of conditions2. The protein must exhibit the following characteristics: Specifically methylates guanosine-37 in various tRNAs..3. A protein that localizes to cytosol and can perform tRNA (guanine(37)-N(1))-methyltransferase activity with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "MGKALEKDIININTINIRDFTEDKHKKVDDYPYGGGAGMVMSLQPIVDSLKRVKESNRGKVIFLGPRGKTFSHEMAKELSKEEELIFLCGHYEGIDERCYDYIDLEISLGDFILTGGEMACIPIVDSICRLIPGVLGKNESFMEESFYEGLLEYPHYTRPENFKGKEVPKILLSGHHENIRKWRKSKSLKITKKRRR",
    "response#2": "MKRAVEKGIIEVHIYNIRDFSNNKHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQMSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGVLNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITKERE",
    "response#3": "MGKALEKDIININTINIRDFTEDKHKKVDDYPYGGGAGMVMSLQPIVDSLKRVKESNRGKVIFLGPRGKTFSHEMAKELSKEEELIFLCGHYEGIDERCYDYIDLEISLGDFILTGGEMACIPIVDSICRLIPGVLGKNESFMEESFYEGLLEYPHYTRPENFKGKEVPKILLSGHHENIRKWRKSKSLKITKKRRK"
  },
  {
    "reference": "MKVVIPSEMSRMEKRTIQEGFLSEDLLMERAGLAVARGVLALSPFPSSVVAMVGKGNNGADAVVALRHLALKSIKTAAILLYPEGEHSKALSLALKRLSVAQGGRPDGILHFSPQTSIHKLRHASVIIDGILGTGFKGEPANEVLVAIETMNALHVPVLSVDIPTGLDGETGRGEKAVQANRTVTFAFPKWGLLVDPGCRYTGDLVVSDIGLYPSLSFPAPSRQLILPEDMATLLPKRPPSLHKGQAGHLLVWGGSDGKWGAPMLSGLGGQRAGAGLLTLLFPHTGPKTSFFPEQMIRSLPESFSEGLSAIFDNIDVVAMGPGLSPGSEELQISRYVLDEFSGPVVADAGIFDLYRNFPMSLVRSNGAPLILTPHPGEFARYLNLPVSTVLEHSTELLLEWSEKLSAIILLKGYRTIIAAPDGRMTVNMTGSPNMATAGSGDVLTGVISGLLAQGTSPYEAVILSAYLHGLAGAELAKSRSIGILARELADSVGLVLSAAATEMDQSVPHDPWGDILLTRPARPILPTTGGPYEKI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for K(+), AMP binding.2. The presence of Mg(2+) is necessary for the protein to function in the desired environment.3. The protein design must be able to perform NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity in order to contribute to phosphorylation.4. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. The designed protein sequence is ",
    "response#1": "LTGKWAKDALPERPLNANKGTFGKVLTVAGSVNYSGAAYLACSGAMRAGAGLVTLAVPVNLQPVLAGKLNEVTYLPLSESVKGVVSGEASFIVLSSLHEYTTLLIGCGLGQHDMTVSFTKGILFGNKPIMPLVIDADALNVLAKTNDWWQKLMMDAILTPHPGEMSRLTGIKIEEIQSNRIDTAMLFAQKWGKTVVLKGAYTVIASADGRCRVSPFANPGMASAGTGDVLAGIIAGLTAQGLDNFNAACLGVWLHGQAGEIVKAELGDTGMLAGDLLQVVPKAIADGNKGTFGKVLLIAGSMNMCGAAILSGKAAFRSGCGMVKIMTVEENRVILQESFPEAMLLTYTEDGFDSEELSRSMDWCDCIGIGPGLSQGETALRLVEFILANAAVPVVADADALNIIAKRPEILSSCQTEVVVTPHIGEFGRLTGLETEEIKTHFYDVAADFARRFSVVCVLKDARTIIVNKDGEAFINCTGNSGMATAGSGDVLTGIICSLIAQGLGPFAASSLGCALHGRAGENGSAFRSPTELMAGDIIEGVL",
    "response#2": "MYEDQEIRFPERRADGNKGTFGKVLLIAGSMNMCGAAILSGKAAFRSGCGMVKIMTVEENRVILQESFPEAMLLTYTEDGFDSEELSRSMDWCDCIGIGPGLSQGETALRLVEFILANAAVPVVADADALNIIAKRPEILSSCQTEVVVTPHIGEFGRLTGLETEEIKTHFYDVAADFARRFSVVCVLKDARTIIVNKDGEAFINCTGNSGMATAGSGDVLTGIICSLIAQGLGPFAASSLGCALHGRAGENGSAFRSPTELMAGDIIEGIVRDGHKYLRGHCLVVAGGPLSTGAARLSASAALRSGAGLVTLAAEPAAASVIANHVTAEMVLSFTNGPDLVGILRDERKNVVVYGPGAGRMTDTQDRTAAILESRAHAVLDADALSAFEDGPERLFALLRRRANADAVLTPHEGEFERLFPGLRGRAVNKIEAARIAAEQAKAVIVLKGADTVIAHPDGRAQVNTNAPPWLATAGSGDVLAGVIGALLAQGRPAFEAAWSGVWIHGAAARTLGPGLTAADLPEAIPSILS",
    "response#3": "MYEDQEIRFPERRADGNKGTFGKVLLIAGSMNMCGAAILSGKAAFRSGCGMVKIMTVEENRVILQESFPEAMLLTYTEDGFDSEELSRSMDWCDCIGIGPGLSQGETALRLVEFILANAAVPVVADADALNIIAKRPEILSSCQTEVVVTPHIGEFGRLTGLETEEIKTHFYDVAADFARRFSVVCVLKDARTIIVNKDGEAFINCTGNSGMATAGSGDVLTGIICSLIAQGLGPFAASSLGCALHGRAGENGSAFRSPTELMAGDIIEGIVG"
  },
  {
    "reference": "MASSYLSLAARLSQCWISPWSLCCLYILMQFFLFTKDLNTKIGDFVNDEQATCNYIQEKVDILLDSPSLIANAAVRVAKDGIQSTVKIILSGISDSLIAAENVFIFFIEFSYGTYLCLIQLAIDGILDAVADVGEEIGTAVNDTLHAIADEIEDTVSSLNEVFQSAEDSLEKVASWLGEDINLPNVSIPEIQSLRNFTLSSSYDTEFEKLKAGVNFDSAINATKAAISKPFSSARNLILEKVSNYSFDTSMVSSPNKTHVVVCSTDDLTAISSFILSSIYKIRKVVIISLLIIIAGLFLISSIYEIWKWCRIRHKAFLLDEHIRSNKFEDTRDLISYIESPISWNLKYFISALPLPCFLSVQLRWFITYIFHPPAAMILFISCTSFISGILQLVLLNNIREDGSVISALAQNSFHKVESALANVSVAWANSTNQIILKNQENINNNMFGSIHNTTLSLNSTLNTFMNELNSSMTSAFGDTFLASTVQNVMNCLLYRKIENFEEVLTWVYNKSHIELPLLPTDILSKSIDNQTIYSSLYSSLNSSNSTVSFSGIFDRVEKSVISELNFSFLFFLLWLLICAFGLIGVLSSWLKSLFLSLLDLVIPNPKENITLPVQSLAFPVTKSCRPPPIPPRESHVYDFQNFQYEEDDCIDYKRSLGLISLSSDLAIDIPISPAIISDIQFNSITTESEETTYLLKEKQDRY",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have a specific function in regulation of plasma membrane sterol distribution, plasma membrane fusion involved in cytogamy and should be localized to plasma membrane, cytoplasm, mating projection tip in order to carry out this function efficiently.2. For general function, the protein need meet that Involved in cell fusion during mating by stabilizing the plasma membrane fusion event.3. The protein should have a Helical domain that is long enough to span the entire membrane.4. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "MGAASMALTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARILAVERYLRDQQLLGLWGCSGKLICTTNVPWNSSWSNKSHNEIWGNMTWMEWEKEIDNYSDVIYRLIEESQNQQEKNEKDLLALDSWASLWNWFDISNWLWYIRIFIMIVGGLIGLRIVFTVLSIVNRVRKGYSPLSLQTR",
    "response#2": "YSHKEEVIKEVQKFYEDTYNKLKNKDEPQRETLKAIHIALDCCGLTGVPEQFLTDTCPPKNLIDSLKTRPCPEAIDEIFRSKC",
    "response#3": "DNLVRIINAVFLIISIGLISGLIGTQTKHSSRVNFCMFAAVYGLVTDSLYGFLANFWTSLTYPAILLVLDFLNFIFTFVAATALAVGIRCHSCKNKTYLEQNKIIQGSSSRCHQSQAAVAFFYFSCFLFLIKI"
  },
  {
    "reference": "MTLSVLEPAAPAAEPVVSSRISLVGRTRAGLGDALAEIGVPEKERRMRVGQLWNWIYHYGVRDFSAMTTIGKGLRAALDQRFTLDLPEVTAEQVSTDGTRKWLIRMAPTGPRDRGAEIECVYIPEVDRGTLCVSSQVGCTLTCTFCHTGTQRLVRNLTCEEIVAQLMVARDRLGDFPNRSKPDGAFVPNDGGRFVSNIVFMGMGEPLYNFDGVRDAIGVISDNEGLSLGRRRITVSTSGVVPQIGPLGHEMGTMLAISLHAVRDELRNELVPLNKKYPIAELLEACRTYPGVSNAKRITFEYVMLKGVNDSDADARELVRLLKGIPAKINLIPFNPWPGTKYECSDWDRIERFSEIVFRAGYASPVRTPRGRDILAACGQLKSETEKLSARARLMLDQDEAAAAAAVAAE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity.2. The protein requires [4Fe-4S] cluster to activate a critical catalytic site.3. The protein can be targeted to cytoplasm and perform rRNA binding, tRNA binding.4. The protein must exhibit the following characteristics: m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.5. The protein should have a high affinity for [4Fe-4S] cluster. The designed protein sequence is ",
    "response#1": "VEDRGAVCISSQVGCTLSCRFCHTGTQTLVRNLGAPEIVGQFMAARDSYGEWPSPKGETPRLLSTIVLMGMGEPLYNYTNVAKAMKIVMDGEGIGLSRRRITLSTSGVVPMMDRAGAELGVNLAVSLHAVTDELRNELVPLNRKYPIAEVLAACRRYPPASNARRITFEYIMLKGVNDSEAEARELVRLIAGLPAKVNLIPFNPWPGSKYEVSGKAALDRFAAIVMAAGFSAPIGRGDIEIKSADE",
    "response#2": "VEDRGAVCISSQVGCTLSCRFCHTGTQTLVRNLGAPEIVGQFMAARDSYGEWPSPKGETPRLLSTIVLMGMGEPLYNYTNVAKAMKIVMDGEGIGLSRRRITLSTSGVVPMMDRAGAELGVNLAVSLHAVTDELRNELVPLNRKYPIAEVLAACRRYPPASNARRITFEYIMLKGVNDSEAEARELVRLIAGLPAKVNLIPFNPWPGSKYEVSGKAALDRFAAIVMAAGFSAPIR",
    "response#3": "DDERGTLCVSSQVGCSLTCSFCHTGTQRLVRNLTPGDIVGQFMKARDDLGDWERSDAGRRLTNIVFMGMGEPLYNTDNVIAALRLLGDGNGLGMGRRRITLSTSGVVPELRRIGEALAPQLAISLHAVTDELRNELVPINRKYPIAELMRAVRDYPTLKNAQRVTWEYVMLDGVNDSDADARALVRLIEGIPSKINLIPFNPWPGSAYERSSDERIRRFSRIIMDAGYASPVRTPRGQS"
  },
  {
    "reference": "MIPNGYLMFEDENFIESSVAKLNALRKSGQFCDVRLQVCGHEMLAHRAVLACCSPYLFEIFNSDSDPHGISHVKFDDLNPEAVEVLLNYAYTAQLKADKELVKDVYSAAKKLKMDRVKQVCGDYLLSRMDVTSCISYRNFASCMGDSRLLNKVDAYIQEHLLQISEEEEFLKLPRLKLEVMLEDNVCLPSNGKLYTKVINWVQRSIWENGDSLEELMEEVQTLYYSADHKLLDGNLLDGQAEVFGSDDDHIQFVQKKPPRENGHKQISSSSTGCLSSPNATVQSPKHEWKIVASEKTSNNTYLCLAVLDGIFCVIFLHGRNSPQSSPTSTPKLSKSLSFEMQQDELIEKPMSPMQYARSGLGTAEMNGKLIAAGGYNREECLRTVECYNPHTDHWSFLAPMRTPRARFQMAVLMGQLYVVGGSNGHSDDLSCGEMYDSNIDDWIPVPELRTNRCNAGVCALNGKLYIVGGSDPYGQKGLKNCDVFDPVTKLWTSCAPLNIRRHQSAVCELGGYLYIIGGAESWNCLNTVERYNPENNTWTLIAPMNVARRGAGVAVLNGKLFVCGGFDGSHAISCVEMYDPTRNEWKMMGNMTSPRSNAGIATVGNTIYAVGGFDGNEFLNTVEVYNLESNEWSPYTKIFQF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: (Microbial infection) Involved in the alternative splicing of influenza A virus M1 mRNA through interaction with HNRNPK, thereby facilitating the generation of viral M2 protein..2. The design objective is to achieve a protein with strand-helix-strand-strand-helix-helix-strand-strand-helix-strand-helix-helix-helix-strand-strand characteristics.3. The protein should be optimized for stability and solubility when expressed in spliceosomal complex, cytoskeleton, transcription regulator complex, nucleoplasm, cytosol where it will be involved in transcription by RNA polymerase III, response to virus, negative regulation of protein ubiquitination.4. The designed protein must have at least one BTB, BACK domain. The designed protein sequence is ",
    "response#1": "SYNILADTYAQTEFSRTVLYPYCAPYALELDYRQNLIQKELTGYNADVICLQEVDRAVFSDSLVPALEAFGLEGVFRIKQHEGLATFYRKSKFSLLSQHDISFYEALESDPLHKELLEKLVLYPSAQEKVLQRSSVLQVSVLQSTKDSSKRICVANTHLYWHPKGGYIRLIQMAVALAHIRHVSCDLYPGIPVIFCGDFNSTPSTGMYHFVINGSIPEDHEDWASNGEEERCNMSLTHFFKLKSACGEPAYTNYVGGFHGCLDYIFIDLNALEVEQVIPLPSHEEVTTHQALPSVSHPSDDLALPAIAVIGDQSSGKSSVLEALSGVALPRGSGIVTRCPLVLKLKKLVNEDKWRGKVSYQDYEIEISDASEVEKEINKAQNAIAGEGMGISHELITLEISSRDVPDLTLIDLPGITRVAVGNQPADIGYKIKTLIKKYIQRQETISLVVVPSNVDIATTEALSMAQEVDPEGDRTIGILTKPDLVDKGTEDKVVDVVRNLVFHLKKGYMIVKCRGQQEIQDQLSLSEALQREKIFFENHPYFRDLLEEGKATVPCLAEKLTSELITHICKSLKR",
    "response#2": "DLALPAIAVIGDQSSGKSSVLEALSGVALPRGSGIVTRCPLVLKLKKLVNEDKWRGKVSYQDYEIEISDASEVEKEINKAQNAIAGEGMGISHELITLEISSRDVPDLTLIDLPGITRVAVGNQPADIGYKIKTLIKKYIQRQETISLVVVPSNVDIATTEALSMAQEVDPEGDRTIGILTKPDLVDKGTEDKVVDVVRNLVFHLKKGYMIVKCRGQQEIQDQLSLSEALQREKIFFENHPYFRDLLEEGKATVPCLAEKLTSELITHICKSLR",
    "response#3": "LALPAIAVIGDQSSGKSSVLEALSGVALPRGSGIVTRCPLVLKLKKLVNEDKWRGKVSYQDYEIEISDASEVEKEINKAQNAIAGEGMGISHELITLEISSRDVPDLTLIDLPGITRVAVGNQPADIGYKIKTLIKKYIQRQETISLVVVPSNVDIATTEALSMAQEVDPEGDRTIGILTKPDLVDKGTEDKVVDVVRNLVFHLKKGYMIVKCRGQQEIQDQLSLSEALQREKIFFENHPYFRDLLEEGKATVPCLAEKLTSELITHICKSLSPTRRSLTTPCLAPRYSLRAYCSILYLKPRMQIILRGQKVKTQLVSKSLAYIERDVYRPKFLSKTVRITFGFNCRNKDHYGIMMYHRNRLIKAYEKVGCQLRANNMGVGVVGIIECNFLKPTHNKQDFDYTNEYRLTITALGEKLNDYYSLRAYCSILYLKPRMQIILRGQKVKTQLVSKSLAYIERDVYRPKFLSKTVRITFGFNCRNKDHYGIMMYHRNRLIKAYEKVGCQLRANNMGVGVVGIIECNFLKPTHNKQDFDYTNEYRLTITALGEKLNDYDLALPAIAVIGDQSSGKSSVLEALSGVALPRGSGIVTRCPLVLKLKKLVNEDKWRGKVSYQDYEIEISDASEVEKEINKAQNAIAGEGMGISHELITLEISSRDVPDLTLIDLPGITRVAVGNQPADIGYKIKTLIKKYIQRQETISLVVVPSNVDIATTEALSMAQEVDPEGDRTIGILTKPDLVDKGTEDKVVDVVRNLVFHLKKGYMIVKCRGQQEIQDQLSLSEALQREKIFFENHPYFRDLLEEGKATVPCLAEKLTSELITHICKSL"
  },
  {
    "reference": "MKLGIIILAAGQGTRMRSALPKVLHDLAGRPLLQHVIDTALELQPELIALVYGHGGELLCQSLPDAPVVWVEQAERLGTAHAVAQAMEAVAGLDRLLVLYGDVPLIRAETLRHLLDCAEGGLGLLTAELADPGGYGRIVRNDLRHVQRIVEHKDADAEELAIREVNSGIMSLDRAQLQGWLQRIGNDNAQGEYYLTDVVGLAVADGVPVEGVVIADPNEILGVNDRVQLADLERRYQHRAAEGLMRAGVSLRDPARFDLRGRLQAGQDCLIDINVIIEGEVVLGDGVQIGAHCLLKDCQIAAGTQVLPHCVIDQALIGPDCRIGPFARIRPGTELAGHNHIGNFVETKNAQVAEGSKINHLSYIGDSQIGRKVNVGAGTITCNYDGANKHRTRIGDGAFIGSNSALVAPVEIGAGATIGAGSVINKNAPDGELTLTRAKQMTLKGWKRPQKEQK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).2. The presence of Mg(2+) is necessary for the protein to function in the desired environment.3. The protein should be able to bind UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA ligand in a variety of conditions4. A protein should be able to manipulate the activity of enzymes in the Nucleotide-sugar biosynthesis pathway, either by activating or inhibiting their function.5. The designed protein should have magnesium ion binding, glucosamine-1-phosphate N-acetyltransferase activity to facilitate cell wall organization. The designed protein sequence is ",
    "response#1": "VVILAAGKGTRMQSALPKVLHPVAGLPMAEYALRATAPLDAASTTVVVGYGAERVQAALSQWEVAFATQAPQLGTGHALTQAESALAGAKGTLVLLAGDVPLLRAATLRTLVATHQQAGAAATVLTATVPRPFGYGRIIRNQNGEVRRIVEEKDATDQERAIREINTGVYVFAAEILRTHLPLIKPTNAQGEYYLTDIIALARLRGLGVAGALVENPYEAMGINNRLQLAEAERILQKRRLLEWQRAGVRLADPARFDLRGRVHAGRDVEIDVDVILEGEVTLGDGVRIGPFCRIRNATLGPGTVVHAHCDIDGANAAGGCLIGPFARLRPGTVLAAGAHLGNFVEAKNARIGAGSKANHLSYLGDAEIGAEVNVGAGTITCNYDGVRKHRTVIGDGAFIGSNSALVAPVTIGAGATIGAGSVIGKDAPPGELTVARARKRNLIEGWVEEKRLATRKP",
    "response#2": "AIVLAAGQGKRMKSKLYKVLHPVAGKPMVGHVVDTLQHIEVAKTVVVVGYGAEAVQAYLGEQVDYALQEQQLGTGHAALQAREALGAEDGLTVVICGDTPLISAETLQHTIALHRNTGAAATILTAKLDEPTGYGRIIRGEDGRVVRIVEQKDCTPEEVATQEINTGTYIFDNRKLFRALGSVTNNNAQNEYYLTDVIGILTESGDIVLTDDADEILGVNDRAQLAEAAARLRRRRQRELMEAGVTVVDPASTFVDVTVSFERDVVVHPGTQLLGSTSLAEGCEVGPNSRLTDTSVGAGARVDNSVALSAVVGAEASVGPFAYLRPGTRLGRASKAGTYVEMKNASVGEGTKVPHLSYVGDATIGDHTNIGAASVFVNYDGVNKHHTTVGSHCRTGSDNMFVAPVTLGDGVYTAAGSVITKDVPAGSLAVARGQQRNVEDWARRNRPGTPAAEAAERALGAEEQE",
    "response#3": "VVILAAGKGTRMQSALPKVLHPVAGLPMAEYALRATAPLDAASTTVVVGYGAERVQAALSQWEVAFATQAPQLGTGHALTQAESALAGAKGTLVLLAGDVPLLRAATLRTLVATHQQAGAAATVLTATVPRPFGYGRVIRDDAGRIIAVVEEKDASKEERAINEVNTGILAAPTEQLKRWLARLDNRNAQGEYYLTDIVAMAVDDGVPVHSAQPTHEWETLGVNSKVQLAELERIHQQQMAARLLEQCAVDITDPEMTFIDWRLGVTIEDPATTYVDTDVTVGADTVLHPGVTLEGRTQIGSACEIHSGVRIVDSTIADGVTINNHCVITDARVAAGARIGPFAHLRPEADIRRDAKIGNFVEVKKAVVGAGSKANHLAYLGDATVGEGVNVGAGTIICNYDGTTKHHTVIEDGAFIGSDTQLIAPVTVGKGAYVASGSSITDDVPAGSLAVARGRQRNIDGWKRPTKKKT"
  },
  {
    "reference": "MSVMHTDPVLSCEESLEFEKKYFKGDGELEWQSMNKAGQGVGDALLRDMRELRTIPHRPRLLVLVGKGHNGGDSIVATKRFLRTIPTARAVILPLETWEKCRSLTQRAWDELRELAGKRIEVIDPSSNLLKELEEKVKDRGFDALIDGLLGMQAQLPLRDPIPDIIKWVNHSEKIAVRASVDMPTGVTGEGFENPLRADFTYCTGIVKAPVLDSLHAEFVGRLRYLNLDFFTHDRMQEKKLRVLHPNALRKLRKLRPVVGDKKSHGHLFLLAGSRELGGAAMMAAEGALKAGAGLLTVGIPESLHASFVAKLPEAMWVPLPETPDGGLALEGLGTIRKYLDRATALASGPGLGANRETHSLVREVCNLFAGPILLDADAIRPEILEKLNQITDRLVITPHPGEFIRLAGKTGPEEWMRENSCTLVLKGAHTEILSPEVHLFCLGGSSILARGGSGDLLTGIIGALLAKGTFCACESATLAVQWHGRAAEALARQHGQESIRTTEILTYLSFALRNDF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP.2. The designed protein should have ADP-dependent NAD(P)H-hydrate dehydratase activity to facilitate nicotinamide nucleotide metabolic process.3. The protein must bind to Mg(2+) in order to perform its enzymatic function.4. The designed protein should contain one or more YjeF N-terminal domains that are essential for its function.5. The designed protein should have a binding site that is specific to the target (6S)-NADPHX ligand. The designed protein sequence is ",
    "response#1": "MKACEKRSEELGFPASELMANAGKVLASHVISETRREDQPDTPEVVILAGKGNNGGDGLVAANFLNKAGISTKIVLCCGKPATPAAINAYDSLNKDIDVLFYDGDNSHPWAAAIDDASLVVDAIFGTGFTGEIERDLQPLFIKLNTYARRVIACDIPSGVDARSGQAGVLAVQADITVTMHAKKLGMLLSPAKYFCGYIRVEDIGIPAAATEDAATWLKRTRFSSDSYKNKRGHALLIAGSENYSGAAVLAANAAMRSGVGLVTLVTPGSSKVSVESRVLPEVIVRGAAETDGGAIAEEAYDELGNLIAKADAVAIGSGMSQGESTAAFVRRAVAECRQPLILDADALNVLAPLGANWPTADAAEPPKGGTPNVILTPHEGEFLRLLGDEENHRDGETPPPLLRKERSKIKDRVAAVREFAQTHDVILVLKGERVLIGHPDGRVVVNPTGNSGLGKAGNGDTLAGILAGFVAQAAKMDTDIFETVVAAVYIAGTAGDIAETKFGKRVMTASDVRECLTEVFAELEGQP",
    "response#2": "MKACEKRSEELGFPASELMANAGKVLASHVISETRREDQPDTPEVVILAGKGNNGGDGLVAANFLNKAGISTKIVLCCGKPATPAAINAYDSLNKDIDVLFYDGDNSHPWAAAIDDASLVVDAIFGTGFTGEIERDLQPLFIKLNTYARRVIACDIPSGVDARSGQAGVLAVQADITVTMHAKKLGMLLSPAKYFCGYIRVEDIGIPAAATVIIGGSQGKIGAVNLAARACLVAGSGLVTAFVPQCGLLPLQSSAPEIMVLTDMDDKKLTSIALNIKATAYGIGMGMGTAKETRKAFKSFLENYQNPMIIDADALNILSQQPELLKLLPENSVLTPHPKELERVIGKWDDDFNKLEKVKAFTKKHHCIMVVKGAHTIIVDGNDFYVNSSGNPGMATAGSGDVLTGLLTGLLAQGYQPLNAAIFGVFLHGLAGDIAVSNKGYEALIASDLIEIRAWDKYTIENEPISSIDLMERAATKLTDWYLENCSNFKEVTIFCGVGNNGGDGLVMGRLLSNEGCNVTCYIVHFSENQSKDFQENLERLPKRVSCHHIINEEDIPSLEKDTVVIDCIFGSGLSRAPKGITQKTIVTINQSNAPVISIDIASGLYADHYSSHFNSIIEADITLTLETPKLSMLFVENAKNVGEMIVIPIQLSNQYLDSYKNKRGHALLIAGSENYSGAAVLAANAAMRSGVGLVTLVTPGSSKVSVESRVLPEVIVRGAAETDGGAIAEEAYDELGNLIAKADAVAIGSGMSQGESTAAFVRRAVAECRQPLILDADALNVLAPLGANWPTADAAEPPKGGTPNVILTPHEGEFLRLLGDEENHRDGETPPPLLRKERSKIKDRVAAVREFAQTHDVILVLKGERVLIGHPDGRVVVNPTGNSGLGKAGNGDTLAGILAGFVAQAAKMDTDIFETVVAAVYIAGTAGDIAETKFGKRVMTASDVREC",
    "response#3": "MREMDRRAVEHYGIIEALLMENAALATCSVILREFGLAGKRFSVVCGMGNNGGDGLAIARKLHSNGGIVSVYLLGDPARLSGAAKLNYEIASRMPFTMESIQSAESVRLKLLHADVIVDAVFGTGLARNVEGMYRDVIDCINAAGKLVFSVDIPSGINGDTGQIMGAAVNANITTTYGLPKRGTILYPGHERCGSLYVSHISFPPELYGTAALGGLLPRREYSTYKNQCGRIGVAAGSRGFIGAALMTSQGALRAGAGLVEVFVPEDIYEIVASAAPMEAMIKPLQSYRDLLKEKADVWALGPGLGKSRAAEILELIEKTKQPMVIDADGLNILAEKTSTLRRCKGKRLLTPHPGEMKRLWPNEKETRARAAMKFCHRFPVTLLLKGSRTIVAERDRPLSYNTTGNPGMATGGMGDILTGVCAGLAGQGLSLYDAARLGAWLCGRAAEMAIFNGHQSEQSLLPRDLLEYLGEAFKEIG"
  },
  {
    "reference": "MPLCSTEEAIAEIRQGKMIILVDDEDRENEGDLTMAAEFVTPEAINFMAKFGRGLICLPMSGEMADKLQLPLMAKHNGSRFGTNFTVSIEAREGITTGISAADRATTIKAAVADGARPDDLVTPGHVFPLRARPDGVLARAGQTEGSVDLARLAGLKPAAVICEIMKDDGTMARMPDLEVFAAEHGLKIASVRDLICYRMRRGQVSVKCDAKAHLPSLYGDFTVYAYESDREPGTHLALVKGDITTPEPVLVRVHSQCLTGDALGSLRCDCRGQLAAALRQIEKEGRGVLLYMRQEGRGIGLANKIRAYALQDQGYDTVEANRKLGFPDDLRDYGTGAQILVDLGITKIRLLTNNPKKIVGLSGYGMEIVERVPIEIEACPENESYLRTKKEKMAHLLTGIRCH",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..2. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity.3. The presence of GTP cyclohydrolase II domains is critical for the interaction of the designed protein with its intended targets.4. The designed protein must have a Nucleophile; for GTP cyclohydrolase activity that is structurally stable and resistant to denaturation.5. The GTP, D-ribulose 5-phosphate, Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.6. The designed protein must possess 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, manganese ion binding, magnesium ion binding, GTP binding to support riboflavin biosynthetic process under certain conditions.7. The designed protein should interact with enzymes and substrates within the riboflavin biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4, Cofactor biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "VKLPTEHGEFEFIPFRQTSNGLEHAALVKGKWTRDEPVLVRLHSSCFTGDIFGSLRCDCGSQLHKAMEMVEAAGKGVVIYLSQEGRGIGLFNKIKAYKLQDQGMDTVQANIKLGFGEDERDYGVGASIMRELGLGKIRLISNNPVKRAGLEGYGIKIVEAVPASEVFGEREVEIQLPTEYGDFRAVGYTEKLTGKEHVALIKGEVNDGEPVLVRVHSECLTGDVFGSNRCDCGPQLHAALMQIEKEGRGVLLYMRQEGRGIGLINKMKAYKLQEQGYDTVEANEQLGFADDLRDYGIGAQILRDLGIQEMRLLTNNPRKITGLKGHGLEVVERVRVTTIDFPTTYGDFKLHLYRSDIDDHHHLALTKGDVTGKKNVLVRVHSSCLTGDVFGSMRCDCGDQLHRAMERVEQEGMGVILYMRQEGRGIGLANKLLAYELQEHGRDTVEANQELGFKADLRDYGIGAQILVDLGLTSIRVLTNNPKKVVGLKGYGLEITERVPIENGRVTTIDFPTTYGDFKLHLYRSDIDDHHHLALTKGDVTGKKNVLVRVHSSCLTGDVFGSMRCDCGDQLHRAMERVEQEGMGVILYMRQEGRGIGLANKLLAYELQEHGRDTVEANQELGFKADLRDYGIGAQILVDLGLTSIRVLTNNPKKVVGLKGYGLEITERVPVDEVF",
    "response#2": "VAEAHLPSVYGGEWKAIAFENDVDHLDHIALVKGEFAKGESILVRVHSECLTGDVLGSKRCDCGEQLHRAMEMIEREGKGVILYMRQEGRGIGLINKLRAYELQDRGRDTVEANLELGFKADLRDYGIGAQILLTLGITKIRLLTNNPKKLIGLQGYGIDIVDRSHDSYVAVPLPTRFGNFNSHLFKSELDGKEHLVLSRGLSGPGLDGPWKACDEPVMVRVHSECLTGDVFHSLRCDCGPQLDRALEILGGQERAVLVYMRQEGRGIGLENKLKAYQLQDQGLDTVEANEKLGFKADLREYGLGAQMLVYLGVRRMRLLTNNPKKIAGLKGYGLEVLEQLI",
    "response#3": "GVDMPTAWGDFELIAYEQTNTQEIHMALIKGKWEKDEKVLVRVHSSCVTGDIFGSCRCDCGPQLHRAMEMVQEAGKGVVLYMNQEGRGIGLVNKLKAYKLQEEGMDTVQANLALGFPMDKRDYGVGAQILRDLGISKIRLISNNPTKRAGLLGYGLEIVDTVCADMPTQYGHFKIYGYVNDITGEHHVALVKGDIGDGEDVLCRVHSECLTGDVFGSMRCDCGKQLQTAMRQVEAEGRGIILYMRQEGRGIGLINKLKTYVLQEQGFDTVEANIKLGFAPDLREYWIGAQILRDLGVKSLRLLTNNPEKIYGLSGFGLEIHERVPHEEVPERVTEADLPTRYGKFRIYGYVNLINREHHVALAMGNVNDGRPVLCRIHSECLTGDCFGSRRCDCGEQLDTSMRMIAKEGRGVLIYLRQEGRGIGLINKLRAYALQDEGYDTVEANLKLGFAADLREYMTGAQILHNLGAETIRILTNNPQKIGDVSEYGITIAERIPVFPLVKR"
  },
  {
    "reference": "MYPVPTVIEQSSRGERAYDIYSRLLKDRIIMLSGPIEDNMANAIIAQLLFLDAQDSGKDIYLYINSPGGVVTAGLAIYDTMNFIKSDVQTIVMGMAASMASVLASSGTKGKRFALPNSEILIHQPSGGAQGQQTEIEIVAEEILKTRKKINQILADNSGQSVEKLNHDTERDNYLSAQEAKDYGLIDDIMENNKLK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Plays a major role in the degradation of misfolded proteins.; Has a chymotrypsin-like activity The designed protein sequence is ",
    "response#1": "EIMAPPGMEEMTNQLQGLFQNLAGDTKKKRKMKIKDALKALVEEEAAKLVNQEELKEQAIYNVENNGIVFIDEIDKICKRGEVSGPDVSREGVQRDLLPLIEGSTVSTKHGMVRTDHILFIASGAFQVAKPSDLIPELQGRLPIL",
    "response#2": "EIMAPPGMEEMTNQLQGLFQNLAGDTKKKRKMKIKDALKALVEEEAAKLVNQEELKEQAIYNVENNGIVFIDEIDKICKRGEVSGPDVSREGVQRDLLPLIEGSTVSTKHGMVRTDHILFIASGAFQVAKPSDLIPELQGRLPIL",
    "response#3": "TKTHKTTVSGAWAPLIAEESDKLLDQEALTQEALELAENHGIVFLDEIDKVASSSQRSGADVSREGVQRDLLPLIEGTTVSTKYGPVKTDHILFIASGAFHVAKPSDLLPELQGRLPIL"
  },
  {
    "reference": "MIPGEFKFGQGKILCNADKKAITIEVKNTGDRAVQVGSHYHFYEVNSALDFDRKLAWGKKLDIPSGAGVRFEPGDVKKVNLVDFTGERRIFGFHDEVNGYLD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should interact with enzymes and substrates within the urea degradation, CO(2) and NH(3) from urea (urease route): step 1/1 pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MEKAPKR",
    "response#2": "MGSM",
    "response#3": "ETTDA"
  },
  {
    "reference": "MAGNSIGQVFTVTTCGESHGAGLLAIVDGVPPGLALSEADLQIDLDRRKPGTSKYSTQRRESDEVEIISGVFEGKTTGTSIGLLIRNTNQKSKDYGEIKDTFRPGHADYTYSMKYGFRDYRGGGRSSARETAMRVAAGAIAKKYLLERLGVQIRGHVTQIGHEYSDVLNSSTIDWDFVNSNPFFCADADAVSRFETLIDSLRREGTSCGARLEIIASGVPVGLGEPVFDRLDADIAHAMMSINAVKGVEIGDGMAVAGQFGHSSRDEMTPDGFTANHAGGILGGISSGQDIRVSIALKPTSSITTAGKSINTEGEAIEMLTKGRHDPCVGVRATPIAEAMLAIVLLDHYLRHRGQNADIKQPVQSIT",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be designed to have high stability and activity towards 5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate +   phosphate.2. The protein should be optimized for activity in chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7, Metabolic intermediate biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. For general function, the protein need meet that This reaction introduces a second double bond into the aromatic ring system.; Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis4. The protein should have a high affinity for NADP(+).5. A protein with chorismate synthase activity is needed to enhance amino acid biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "MATRRGESHGEALIAVIEGLPAGLELTEADIQPELDRRKPGQGGVTSRQRREADKVRILSGVFEGVTTGTPIALLIENTDQRSKEYGDIRDTFRPGHADYTYWQKYGIRDYRGGGRSSARETAMRVAAGAIARKILMDLYGVRVISHVTAIGGIATPQADWRQVAWEDLIRNPSFLADPEKVAEIDARIAAEGNSIGAVIKVEGLPVGVGEPVFDRLDADIAQAMMSIGAVKGVEIGAGFAAVAQRGTEHRDEMRPGPDGFLSNNAGGILGGISTGQDIIVRLAVKPTSSIRLAQRTVNRAGEETDIVTKGRHDPTIPPRMVPVAEAMLALVLMDHALRQGAIRRPG",
    "response#2": "MTPDIIIKSGKIINAEGSYEADIAIENGKIVKIASHIQAGEEETKIDARGKWILPGGIDPHVHLAMPFTEDTDDFETRTKAAAAGGVTTIIDFAVPDSGQTLRENLDRRREKAESKAVIDYGFHMIVTDQNLDELEALAEVGISSYKVFLAYKGAFMVKDDQILQMMERAAKTGAIVGVHAENGDVIEQLTREQIEAGKAQPKYYALSRPTAAEIEAVNRACILAQKVGAPLYIVHVSNGEAVDVIRKAREEGKAVYAETCPHYLLLDESRYRTEDYLFKMSPPLRSKEDQQALWKALKDGFIDVVSSDHAPHTRAEKEIEGKAAGSPGVETSIPVLFTRMNEAGLITLERMVEIMCKGPAKIFGLYPRKGVIAVGSDADLVVIDPEKEWTIKSEYMNSAAGYTPFEGFKVKGRAEKTIIRGEMVWDDGEILAAAGQGRVIRAG",
    "response#3": "MAPSELLKAGESHGEALIAVIEGLPPGLPIDEVALQAELDKRKPGQSKFTTQRRKADSVEILSGVFEGLTTGTPIALMIRNEDHRSGSYENIRDLFRPGHADYTYEAKYGFRDYRGGGRSSARETIGRVAAGAIAKKYLHQQYGIVIRGYASQIGEIACDIKDWDEVAHNPFFCADPEKADALDELMRDLAKEGDSIGAVVEVFASGVPVGLGEPLFDRLDADIAYAMMGINAVKAVEIGAGIESVSMRGSENNDQMTPDAQGNTTFATNHHAGGLEGGISNGMPIIMRAVMKPIPSISRPLQSVDLETNEPATATIEGRHDPCVLPRAVPIVEAMVALVLADAMLEQKFALRGDSLATMRDAWPRY"
  },
  {
    "reference": "MELAGKKIILGLSGGIACYKAAELARLLGKAGASVQVAMTDAATHFMTTVTMQALTGKPVFTDQWDGRIANNMAHIDLSREADAILIAPCSADFMFKLAHGACDDLLSTLCLARPAGLPLLVAPAMNVEMWQNPATQRNAARLKEDGIRIMGPGAGEQACGETGLGRMLEPEQLLEEVIASFQPKVLSGKRILLTAGPTFEPIDPVRGITNLSSGKMGFAIARAAREAGAQVTLVTGPTGLQTPYGVTRIDVQTAQQMQDAVLKQVSGQDVFIAVAAVADWRVVGASEQKLKKDAAGTPPQLQLAPNPDILAAVAALPQRPYCVGFAAESENLAQYGEEKRRRKNVPLLVGNIGHQTFGRDDNELTLFEDSGQTTLPRADKQALARQLVVAIARRI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must possess phosphopantothenoylcysteine decarboxylase activity, metal ion binding to support coenzyme A biosynthetic process under certain conditions.2. The presence of DNA/pantothenate metabolism flavoprotein C-terminal domains is critical for the interaction of the designed protein with its intended targets.3. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..4. The designed protein should have a binding site that is specific to the target CTP ligand.5. A protein that is capable of catalyzing reactions within the Cofactor biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "KRIILGVTGSIAAFKAAEWTRSLVKEEGVVTVVMTEAAERFVSALTFAALSGNPVHRDMFGDTPEAIMAHLNLSREADVILVAPATAQTIAALARGAADSLLAAVVLAARIPVVICPAMNSNMLAHPATQHNIRCLEEYGYHIVQPGTGSLACGEVGDGRLADWEVAREKLARLLGDPGGLAGRHVVISAGGTREQLDPVRFLGNASSGRQGVALARTAALRGARVTLVAAHITEPVPSGADVVRVGSTAELAEAMLALQPTADALVMAVAAADFTPERVADGKIKKDGSGTLELRLTETTDVLAALSASGAGPAGQVIVGFAAETADDEAHLLELGRAKLARKGCDLLVLNNVSGGAVFGSPDNSVTIIAADGRVEELPVMPKRDVADVILDRVLPLLG",
    "response#2": "KTVLLCVTGGIACYKSASLASALVKQGANVHVLMTKNATEFIGPHTFESLTGNRVSVDTFDRNYQFQVEHIALADQADVVLVAPATANVLAKLAHGLADDMLTTTVLACNCPKLAAPAMNTKMFENPVTQDNLATLQRYGWEIIQPASGRLACGAVGKGKMPEPEELLAYLQPVLQTGPLSGIKILVTAGPTREPIDPVRFISNRSSGKMGYAIAKMAKRRGAKVILVSGPTNLTPPSGVEVCWVETAEQMRNAVLQKWQDCDVVIKAAAVADYRPETQSSTKIKKKDFDLVLKLRRNPDILEELGKKKKDKIIVGFAAETEDLIKNAQEKLKKKNLDMIVANDVSKTDSGFEVDTNRAILLDAAGQEEELPLMGKAELAHRILDRVQKALA",
    "response#3": "KHILLAITGSIAAYKSCELVRLLKKQEHEVTVAMSEAACTFVTPQTFQALSGNPILTAQGGAGNGMAHINATRAADIMLIAPASANTIAKIAHGIADNIITEMVAARNCPLVLAPAMNVEMWQNPANLRNIKQLQADGVHIMQPAHGEQACGEVGVGRMLEAAEIAAELAPLLQPPLKGKSVLISSGPTEEPIDPVRVITNKSSGKMGAALARAALYLGAKVTVVSGPASEPLPSAATVVKVQTAQQMNTALKEHFENSDICIMAAAVSDYRPLCFSETKIVREEKQKFTLALIPNPDILANLALNKGKRFLVGFSLESDGSEERAIAKMNRKGCDMIIFNRADQALGLDSTRVVFLFPDGRREPLPLMPKYEVAHHLLNFVMEHYSPNR"
  },
  {
    "reference": "MENIKNPKNADDCSDSISKNSHQGVDDSLNQSRSYVCSFCIRGFSNAQALGGHMNIHRRDRAKLRQKLMEDNKDDVVAESDASEVVSLDLNEQQQQQGEALTCDDHDQYVDNDISPKQKLEFWVQESKLDTNDHGKVTEASIDGSSSSHHRDIEVLDLELRLGQSVVKKKTT",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Activation factor which mediates telomerase activity and potentiates responses to auxin through the regulation of BT2; Binds in vitro to the DNA sequence 5'-GACAGTGTTAC-3' of the BT2 promoter..2. The protein should exhibit Polar residues compositional bias.3. A protein that contributes to positive regulation of telomerase activity by functioning in nucleus with DNA binding, metal ion binding, DNA-binding transcription factor activity. The designed protein sequence is ",
    "response#1": "LSEEQEHVIELARRKMNIFYTGSAGTGKSVLLRELIKTLKSTYGTSGAVAVTASTGLAACNIGGITLHSFAGIGLGVGDEAMLLKKVRRSAKHRERWKNIKALVIDEISMIDGTLLDKLDGIAKTLRRSSKPFGGIQIILCGDFFQLPPVTKGDQQIKFAFQSYSWRKAIDATIILKKVFRQQGDHLFINMLNEMRTGIISDD",
    "response#2": "LSEEQEHVIELARRKMNIFYTGSAGTGKSVLLRELIKTLKSTYGTSGAVAVTASTGLAACNIGGITLHSFAGIGLGVGDEAMLLKKVRRSAKHRERWKNIKALVIDEISMIDGTLLDKLDGIAKTLRRSSKPFGGIQIILCGDFFQLPPVTKGDQQIKFAFQSYSWRKAIDATIILKKVFRQQGDHLFINMLNEMRTGIISDSLTCLLSEKAKEFLVENRVQSFYQQELETVESLLSLANQPVIHSACSGQMGFKNDTTSRAIHSIFRNAVKLLQEEGLVFQKDGGFDNLFYVTREDKELHRKIHRIIQEECQKPNHVEKGCHFLHILACARLSLSPGLSEPVLQQVLQLLEDQSDIVSTTEKYYTAC",
    "response#3": "LSEEQEHVIELARRKMNIFYTGSAGTGKSVLLRELIKTLKSTYGTSGAVAVTASTGLAACNIGGITLHSFAGIGLGVGDEAMLLKKVRRSAKHRERWKNIKALVIDEISMIDGTLLDKLDGIAKTLRRSSKPFGGIQIILCGDFFQLPPVTKGDQQIKFAFQSYSWRKAIDATIILKKVFRQQGDHLFINMLNEMRTGIISDH"
  },
  {
    "reference": "MDYLPLFCQLDNKPVLLVGGGEAAERKARLLLAAGARLTLVAPMLCQALADQAASGAILWLPECFVPEHLEGKWLVVAATGLRDVDALVYQSANSRRIFCNVVDDTKRSSAIMPSIIDRSPLMIAISSAGTAPVLARLLRERLEALLPEHLGRMAAFAGTLRERVKARFASHGERRRFWERLLGADRLGQALARGDQAAANQLADTLFASNSELRGEVVLVGAGPGDPGLLTLHALRHLQQADVVVYDRLVSAEVMALARRDARRIFVGKQAGHHCVPQEGINQLLLDEAKRGQKVVRLKGGDPFIFGRGGEELETLLGSGVGFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFVTAHGKGGALDLDWPMLARDRQTLVFYMGLAQCGVIRDQLTAHGRPADTPVALIENGTRPEQRVIRGTLAQLPTLAEGVESPALIMVGSVTTLADKLAWFGEATPGLKASA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.2. The designed protein should be optimized for precorrin-2 dehydrogenase activity, NAD binding, sirohydrochlorin ferrochelatase activity activity in order to improve siroheme biosynthetic process, cobalamin biosynthetic process, methylation.3. The designed protein should contain one or more Siroheme synthase central domains that are essential for its function.4. A protein that is capable of catalyzing reactions within the Porphyrin-containing compound metabolism, siroheme biosynthesis pathway with high efficiency and specificity.5. The designed protein should be able to compete with endogenous proteins or inhibitors for NAD(+), S-adenosyl-L-methionine binding. The designed protein sequence is ",
    "response#1": "VGGGPGDPDLLTFRALRLMQQADVVLYDRLVAPEIVELVRKDAERIYVGKERNQHALPQENINQMLVRLAREGKRVLRLKGGDPFIFGRGGEEIETLSAEGIPFQVVPGITAAGGCASYAGIPLTHRDYAQSCIFVTGHLKDGTMNLNWNALVQPKQTIAVYMGTHGLEVLCSELIKRGLPENTPAAIVQQGTTQQQRVYIGTLKSLPEIPKQYEVKPPSMIIIGEVVT",
    "response#2": "VGGGPGDPDLLTFRALRLMQQADVVLYDRLVAPEIVELVRKDAERIYVGKERNQHALPQENINQMLVRLAREGKRVLRLKGGDPFIFGRGGEEIETLSAEGIPFQVVPGITAAGGCASYAGIPLTHRDYAQSCIFVTGHLKDGTMNLNWNALVQPKQTIAVYMGTHGLEVLCSELIKRGLPENTPAAIVQQGTTQQQRVYIGTLKSLPEIPKQYEVKPPSMIIIGEVLA",
    "response#3": "VGAGPGDAGLLTLRGLQVIQQADVVLYDQLVSADILELVRRDAERICVGKRAGAHSVTQEEINRLLISLARQGKRVVRLKGGDPFIFGRGGEELQQVAQAGISFQVVPGVTAAAGATAYAGIPLTHRDYAQSVVFITGHCRPDGDALDWSTLARGRQTLAIYMGTVKAAEIARQLIAHGRAAETPVAVIGRGTRHDQQVLTGTLRQLEQLARQAPTPALLVIGEVEEALAGAAG"
  },
  {
    "reference": "MHFQVILMMMWLWLEAEGGTCPTLYKRYSKHHTYCLHPNSTCKILTRGVSSGDKEIILREHNKLRSRVATGKETKYSLPKASDMMQLVWDDELASVAQKHADQCVFEHDCNDCRNVQNFGVGQNLHLRTSSAKFSDITWAGAVNGWYDEVKDFNKRQISGFIDGKGPPQTGHFTQAVWATSWRVGCGRSMFKDGNTFKDLYTCNYGPGGNMKNAIIYTKGKPCSGCPLNSCCGKACGGISYDGLCKMSGNTAPQYSPPKGFAFRCTFNGESDCATTTSGAKKWKTIKTLSGSFIGIVLNPGESSTLSFTVPFGVSDGSMCVESYYRKGPQVAGQVAAGNAVEKFGDPADPSFDYSTPLKESTDFMQFGVTLGWNTKTTLSVVFSVPPGTAPQYLEMKDISVRKGEC",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein localizes to the extracellular region.2. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "MCCGSEAYDANSQICCNGNINTKATGPTAQPGCCGEFSYDAASQLCCDSHPVLMVGSLPSCCGRNGYDANTSLCCGDNNVAFVSGPQAACCGDMGYNRNTHLCCDSNVLPMPAMGACCGSWTYSQQTHLCCER",
    "response#2": "MCCGSEAYDANSQICCNGNINTKATGPTAQPGCCGEFSYDAASQLCCDSHPVLMVGSLPSCCGRNGYDANTSLCCGDNNVAFVSGPQAACCGDMGYNRNTHLCCDSNVLPMPAMGACCGSWTYSQQTHLCCER",
    "response#3": "MCCGSEAYDANSQICCNGNINTKATGPTAQPGCCGEFSYDAASQLCCDSHPVLMVGSLPSCCGRNGYDANTSLCCGDNNVAFVSGPQAACCGDMGYNRNTHLCCDSNVLPMPAMGACCGSWTYSQQTHLCCEG"
  },
  {
    "reference": "MIENFDFGRVVIIGDIILDCYINGSVGRISPEAPVPVLLRAHHTAVPGGAANVATNAATLGCEVTLLGLVGCDDSAEILRATLSTTPGIDLTGLVVDSSWTTITKTRVLSGRHQIVRIDEECVVSLPETTQARLIEAARNAISKADVVVCSDYAKGVLSDDVLGAVISFARERGIPVIVDPKRKDFLAYRGASLITPNRSEMTLATGLPMDSDEKVERAARMVAEQFEGDVLVTRSEDGMSLIRREGDVTHVRARKSELCDVSGAGDTVVATIASMLSAGQDLETAVVIATSAASIAVSKLGTATVSRSELSAALLAEIKEDGGTLSIQQAQTVVDSWRHHGARVVFTNGCFDLLHPGHISLISAAARQGDKLIVAINSDASVKRLKGASRPLQDEGARGTVISALRDVDLVVIFDEDTPLEIIQTLKPDVVVKGADYQEYEVVGGDFVKSYGGAVVLVDIVSGRSTTSIVKKMQQTKSAVEMAL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must possess heptose 7-phosphate kinase activity, ATP binding to support ADP-L-glycero-beta-D-manno-heptose biosynthetic process under certain conditions.2. The designed protein should contain one or more Carbohydrate kinase PfkB domains that are essential for its function. The designed protein sequence is ",
    "response#1": "FTNGCFDILHPGHVKVLTAARGACDRLVVGLNSDASTKRLKGKGRPVQDERARAEVLAALEAVDLVVIFEEDTPIKLVAQIKPSVLVKGGDYTRDQVVGHDIVEAYGGEVLLVEVK",
    "response#2": "MPRFDHSSVLVVGDVMLDRYWHGVTSRISPEAPVPVVKVGEKEDRPGGAGNVALNIAALGAPAYLVGVTGDDEASHVLQACLEAAGVWCSFQQVKDEPTITKLRILSRHQQLIRLDFEEAFKSLDEVGFTERFTSLLQNVDVVVLSDYGKGALTKPQQLIQAAKAAGKPVLVDPKGTDFAKYQGATLITPNLSEFETVVGACPDEATLTQKGMALLAELELDALLVTRSEHGMTLLRPSGIPLHLPAQAKEVFDVTGAGDTVIAVIASALGAGLSLPESVALANLAAGIVVGKLGTATVSAPELRRALSEWRRQDLRVGFTNGCFDILHPGHVKVLTAARGACDRLIVGLNSDASVKRLKGEGRPVQDERARAEVLAALEAVDLVAIFEEGTPIELITKIKPSVLVKGGDYTREQVVGHEIVEACGGEVVLIDILPGHSTTSLVDRAR",
    "response#3": "MTGGCFDILHAGHVAYLEQAKRLGDRLIVAVNDDASVSHLKGSGRPVNNLEKRMAVLAGLSAVDWVVPFSEATPERLIKSILPDILAKGGDYQLKDLPEAKIVKAYS"
  },
  {
    "reference": "MRTGDMREFLETGVIDAGRMRAVEGNAVALGYPSLLMMESAGRAVADAVLSRTPSRVLVLCGRGNNGGDGMVAARYLQHLDSVDVVYPDCGTTTPSAATQLALLRHCSVALHTVRCAADVETLSRLFDDADVIVDAMLGTGASGAVREPFASLVARANTNSAPIIAVDIPTPGIRASRIVSFHRPKVEGADVVDIGIPFEAEIFTGPGDLTLIPPKGSKAHKGAGGEVLVVGGGPYQGAPYIAALGALRAGADIVRVASPAYIPAPDLIYERLEGKAITADHLATILPLVDRADTVVCGMGAGRESHDVILAVAEAAKRAVFDADALAKPLPAAKETIYTPHAGEFARMTGAEPPTDLVARGRSVKAAATAGTILLKGPVDVISDGSRVRFNRTGTPAMTVGGTGDLLAGVVGALFCHLPAFEAASLAAYVCGRAGMLAAEGRGNGMLATDMLDGIPEILFLPPAPD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.2. The K(+), AMP binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "LPERVRWGHKGVFGKFLAIAGSRYYYGAPYYTSYSFLKAGGGYSRLAAPKSVIPYIAARCSEVVYIPLEETSEGSIAMNSYEAILRLIEEAGIDIVALGPGVSLNSETQELVRMLVEAIDRPLIIDGDGITAISKDPGRVKGRKAPTIITPHVVEFSRLVGVDLKKIQEDPVGSLRSACRDLGSYIVLKGAHTMICYPDGYIYINMTGNPGMAKAGSGDVLTGAIAAMYGAGYRDPGAAARMGVLIHGLAGDLAAEDLGEDGVTPDDILNYLPKAVAQ",
    "response#2": "AIAGSRYYYGAPYYTSYSFLKAGGGYSRLAAPKSVIPYIAARCSEVVYIPLEETSEGSIAMNSYEAILRLIEEAGIDIVALGPGVSLNSETQELVRMLVEAIDRPLIIDGDGITAISKDPGRVKGRKAPTIITPHVVEFSRLVGVDLKKIQEDPVGSLRSACRDLGSYIVLKGAHTMICYPDGYIYINMTGNPGMAKAGSGDVLTGAIAAMYGAGYRDPGAAARMGVLIHGLAGDLAAEDLGEDGVTPDDILNYLY",
    "response#3": "AWLPAPPVATTRQHAQHKGSFGDVLVVGGSHGMAGAAVLAARAAHAAGAGRVHVDLLDDNAFTHDLVRPELMFRPSERRAALPASATVVAGCGGGDPIAAALPRLLTVSERLVLDADALNALATDPTLQAALTARGGLGLGTVLTPHPLEAARLLGTDTATVQRDRLAAALQLARRFACVVVLKGSGSVVASPDTAPAINPTGNAALATAGTGDVLAGWIAGLWAAHGLTAVEAAERGTWQHGEAADRSPLTPLRAADLVEALHALSRR"
  },
  {
    "reference": "MEAEDYNASYEDYPDDVDPIVVLEELSPLEGRVVRILLVAVYSVICLLGILGNGLVIVMITCKMKRTVNTVWFLNLAVADFLFNVFLPVHIAYAALDYHWVFGTAMCKISNFLLIHNMFTSVFLLTVISFDRCVSVLLPVWSQNHRSVRLAYTACLVIWVLAFFLSSPSLVFRDTARLHGKISCFNNFSLSAAVSSPWPAHPQVDPVGSGRHKVVTITRFLCGFLVPGLITTACYLTIVYKLQRSRLAKTKKPFKIILTIIVTFFLCWCPYHAFYLLELRRGSVPPSVFSLGVPLATAIAIANSCMNPILYVFMGQDFKKFRVALFSRLVNALSEDTGHSSYPSHRSFTKMSSMNERETGML",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Interaction with RARRES2 initiates activation of G proteins G(i)/G(o) and beta-arrestin pathways inducing cellular responses via second messenger pathways such as intracellular calcium mobilization, phosphorylation of MAP kinases MAPK1/MAPK3 (ERK1/2), TYRO3, MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis.2. A protein with G protein-coupled receptor activity, complement receptor activity is needed to enhance negative regulation of interleukin-12 production, positive regulation of macrophage chemotaxis in a specific context.3. The designed protein should have at least Helical; Name=6, Helical; Name=2 transmembrane domains to ensure proper localization.4. The Extracellular topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "RSGAWGIVSEAVAGAGIITTFVLTIILVASLPFVQDTKKRSLLGTQVFFLLGTLGLFCLVFACVVKPDFSTCASRRFLFGVLFAICFSCLVAHVLSLNFLTRKNHGPRGWVIFTVALLLTLVEVIINTEWLIITLVRGGGQVSPLGNVSADSTMTSPCAIANMDFVMALIYVMLLLLTAFLGAWPTLCGRFKRWRKHGVFVLLTTVISIAIWVVWIVMYTYGNEQHHSPTWDDPTLAIALAANAWTFVLFYVIPPEIVNPASELTANVPNKVGTCVSEGSYPAGTLSWHLDGKPLIPDGKGTVVKEETRRHPETGLFTLRSELTVTPAQGGTTPTYSCSFSLGLPRRNRQEREESVVVCTGWLRKSPPEKKLRRYAWKKRWFILRSGRMSGDPDVLEYYKNEHSKKPLRIINLNLCEQVDAGLTFNKKELQDSFVFDIKTSERTFYLVAETEADMNKWVQSICQIL",
    "response#2": "KAEAWGIVLETVATAGVVTSVAFMLTLPILVCKVQDSNRRKMLPTQFLFLLGVLGIFGLTFAFIIGLDGSTGPTRFFLFGILFSICFSCLLAHAVSLTKLVRGRKPLSLLVILGLAVGFSLVQDVIAIEYIVLTMNRTNVNVFSELSAPRRNEDFVLLLTYVLFLMALTFLMSSFTFCGSFTGWKRHGAHIYLTMLLSIAIWVAWITLLMLPDFDRRWDDTILSSALAANGWVFLLAYVSPEIVNPASELTANVPNKVGTCVSEGSYPAGTLSWHLDGKPLIPDGKGTVVKEETRRHPETGLFTLRSELTVTPAQGGTTPTYSCSFSLGLPRRY",
    "response#3": "RSGAWGIVSEAVAGAGIITTFVLTIILVASLPFVQDTKKRSLLGTQVFFLLGTLGLFCLVFACVVKPDFSTCASRRFLFGVLFAICFSCLVAHVLSLNFLTRKNHGPRGWVIFTVALLLTLVEVIINTEWLIITLVRGGGQVSPLGNVSADSTMTSPCAIANMDFVMALIYVMLLLLTAFLGAWPTLCGRFKRWRKHGVFVLLTTVISIAIWVVWIVMYTYGNEQHHSPTWDDPTLAIALAANAWTFVLFYVIPPEIVNPASELTANVPNKVGTCVSEGSYPAGTLSWHLDGKPLIPDGKGTVVKEETRRHPETGLFTLRSELTVTPAQGGTTPTYSCSFSLGLPRRRPLL"
  },
  {
    "reference": "MSEKEQQEQQNTSGNGVEKIRTMIEGFDDISHGGLPVGRTTLVSGTSGTGKTLLSLQFLFNGISFFDEPGVFVTFEESPSDIIKNAHIFGWNLQRLINEGKLFILDASPDPEGQDIVGNFDLSALIERLQYAIRKYKAKRVSIDSITAVFQQYEAVGVVRREIFRLVARLKQLNVTTIITTERSEEYGPVASFGVEEFVSDNVVIARNVLEGERRRRTIEILKLRGTTHMKGEYPFTITNDGVNIFPLGAMRLTQRSSNVRVSSGVKTLDGMCGGGFFKDSIILATGATGTGKTLLVSKFLQNGCVNNERAILFAYEESRAQLSRNAYSWGIDFEELESQGLLKIICTYPESTGLEDHLQIIKSEIAYFKPARIAIDSLSALARGVSNNAFRQFVIGVTGYAKQEEITGFFTNTTDQFMGSHSITDSHISTITDTILMLQYVEIRGEMSRAINVFKMRGSWHDKGIREYNITADGPEIQDSFRNYERIVSGSPTRVSIDEKAELSRIVRRFEDKQGSDS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein have KaiC domains with specific sequence motifs that are crucial for its activity.2. For general function, the protein need meet that Has a weak, temperature-independent ATPase activity; ATPase activity defines the circadian period3. A protein with protein serine/threonine/tyrosine kinase activity, magnesium ion binding, ATP binding, protein serine/threonine kinase activity is needed to enhance regulation of circadian rhythm, regulation of DNA-templated transcription, phosphorylation in a specific context.4. The protein should have a low Km value for ATP + H2O = ADP + H(+) + phosphate.5. The ATP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MVAHELRTPLSAAKLALQSQALGQIDLIKLQEVVKRRLEEIESLSKDLLEVGTTRWEALFNPQEANLANIAAEVILELEKFWLSRGIGINTDIPADLPNVFADQSRMKQVLLNLIENALKFSNDGDTVEITMLHRTNQWVQISVSDKGPGIPEEEQQRIFLDRVRLPQTSNETSGFGIGLSVCRRIVEVHGGKIWVVSQPGEGSCFYFTVPVWR",
    "response#2": "MVAHELRTPLSAAKLALQSQALGQIDLIKLQEVVKRRLEEIESLSKDLLEVGTTRWEALFNPQEANLANIAAEVILELEKFWLSRGIGINTDIPADLPNVFADQSRMKQVLLNLIENALKFSNDGDTVEITMLHRTNQWVQISVSDKGPGIPEEEQQRIFLDRVRLPQTSNETSGFGIGLSVCRRIVEVHGGKIWVVSQPGEGSCFYFTVPVWR",
    "response#3": "MLAHDLRNPLTAAAIAIETLQSNYNLETGEFQRLKPSMTAHLLKQARNQTKVIDRMITDLLQVGRGKDTEFPLSPQKVQLGKVFLDVLEELCDRYTAKSQEVETDIPSDLPYVYADPERIRQVLVNLLDNAIKYTPEGGKISVSGLHRTTQKVQFSIGDTGPGIPVENRDRIFENHFRLQRDEGKEGYGIGLCLCQRIIVAHYGQIWVDSAPNNGAWFHFTLPVYR"
  },
  {
    "reference": "MRVKGTLRNYQQWWTWGVLGFWMLMICNGGGNFWVTVYYGVPVWKEATTTLFCASDAKAYETEVHNVWATHACVPTDPNPQEMVLGNVTENFNMWKNDMVEQMQEDIISLWDESLKPCVKLTPLCVTLNCSNVTHSGIDSKSTYKEEGMKEVKNCSFNATTEMKDRKQKVYALFYRLDIVPLNNNSSKNPKEYYRLINCNTSAIAQACPKVTFDPIPIHYCTPAGYALLKCNNKTFNGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENITDNVKTIIVQLNQSVEIVCTRPNDNRRKSIRIGPGQAFYATGEIIGDIRQAYCNISKAQWHGTLQNVSKKLAEYFPPPKTIQFEPSSGGDLEVTTHSFNCGGEFFYCNTSGLFNGTYNMTNDTNSHSNSIIRLPCRIKQIINMWQKVGRAMYAPPIEGNITCKSNITGLLLVRDGGEPNNIEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVMQKEKRAVGIGAVFLGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGIWGCSGKLICTTSVPWNSSWSNKTQEEIWDNMTWMQWDKEISNYTDTIYRLLEDSQNQQETNEKALLELDSWKNLWNWFDISNWLWYIRIFIMIVGSLIGLRIIFAVLSVVNRVRQGYSPLSFQTPTPNPGGPDRLGRIEEEGGEQDRDRSIRLVSGFLALAWDDLRSLCLFSYHRLR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes. The designed protein sequence is ",
    "response#1": "MGAASVALTVQAKQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLALERYLKDQQLLGIWGCSGKLICTTSVPWNSTWSNKSLESIWNNMTWMEWDREINNYTNIIYGLLEASQIQQEKNEQDLLALDSWANLWNWFDISNWLWYIKIFIMAVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTR",
    "response#2": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGLWGCSGKLICTTNVPWNQSWSNKSIGDIWDNMTWMQWDREISNYTSTIYKLLEESQNQQERNEKDLLELDSWKNLWSWFSISNWLWYIKIFIIIVGGLIGLRIIFGVLSIVKKVRQGYSPLSFQTR",
    "response#3": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGIWGCSGKLICTTNVPWNSTWSNKTKQEIWENMTWMQWDREIDNYTSTIYQLLEESQNQQERNEQDLLALDKWDSLWSWFSITNWLWYIKIFIMIIGGLIGLRIIFAVLSIVNRVRQGYSPLSLQTR"
  },
  {
    "reference": "MSGENTSRAWRERLHGQTPPLPAGKTEADVAVTARPSDAPASKEEEEAAESGESGDNRSRSPSYFSGSDNDDLEAEWSDSVQKYGELFYLELSDNEEESLLSEIPVVNHVRFSENEIIFDEDYLREGRKYEPRLKKFSKILKSRRLLPKRYKKKNSNGPVSILKHQPTQKMGIILQQRYKDVSVFVNPKNAMVLKAKEHLKLLEVLLGIIHQTKWKKRTEGTKLVVHGLLPGGPAMKSGQVLIGDILAAVNDVDVTSENIERVLSCIPGPMQVKLTFEIANARQKEASQPKQKKAQTTSNDLVRLLWGEEVQELQQNLQSIPHIIMYLSLQLDSETLKDEQEILYHYPMSEASQKLKSVRGIFLTLCDMLENVAGTHVTSSSLSLNEKLVHVAYWKEDDKLLLIGLPAEEVPLPQLRSLIEDVAQTLKFMYGSLNSAFCEAENVARLDHFFNLFFQRAIQPEKLYSSTCTQSYDASFSLFTDTLPGVQWLTLPQETQMEIDMVLNDLEAADFAELSEDYYNMRRLYTILGCSLFFKGYLICSHLPKDDLIDITMYCRHYCLLPLVSEQRIGHLVIWREVFPRHHLQPASEPDAQVFQEPEGRYFLLIVGLKHYLLCVLLEAGGCASKAVGHPGPDCIYVDQVKATLLQLEGVEASLDERLASPSNPSLSCADWFLPSSRDKLESLTSSPLLTKLQYTSRAITSPSSRRRLFSDYSLKTRKLSPSRSNGGFENGVEGGEGGGSSPYTTLDPLPKQGAKEAQSPGGPQAS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be able to modulate regulation of ossification in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "VKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAVAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTQ",
    "response#2": "MVFLQGVAGIGKSLMLTKLMLAWSEGMVFQNKFSYIFYFCCQDVKKMKRASLAELISKEWPNASAPIEEILSQPEKLLFVIDNLEVMECDMSEWESELCDDCMEKQPVNILMSSLLRRKMLPESSFLVSATPETFEKIEDRIECTNVKMMAGFNESSIKVYFYSLFQR",
    "response#3": "HPSIQEVCAAIFHLLKSHVDHPSQDVKSIEALIFTFLKKVKIQWIFFGSFIFGLLHESEQKKLEAFFGHQLSQEIKRQLYQCLETISGNKELQEQIDGMKLFYCLFEMDDEAFM"
  },
  {
    "reference": "MKLTRVLIIAVLFLTAYQLTTVETYSRGKWMHRALRSTGKNPKVTRECSSPDESCTYHYNCCQLYCNKEENVCLENSPEV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit toxin activity, ion channel inhibitor activity. The designed protein sequence is ",
    "response#1": "KLCILHRNPGRCYDKIPAFYYNQKKKQCERFDWSGCGGNSNRFKTIEECRRTCG",
    "response#2": "MGLIP",
    "response#3": "GFDWSGCGGNSNRFKTIEEC"
  },
  {
    "reference": "MDQGGVKKIAYRGNDHLSKLVYTESEEGGLLKVAPHSAVSLTSATSVVLPTSPLMQPGQVTNGLSNSPLPDELTSASAPATIGSLVESPEPRGLTKDQRPQQPHQFQTQTSNAFGHQSLPQLEASIVDITQSSMDSLIGGSDPNLFAMKTEDFSLDKGEQDSTDLVNAFDSIGKDVDMNQKLFNDTSLDLLQEFDLTGSPSDFYVGDDAFLTTLADDSLLVDGDMRGTLEKDIKPTLVESINTTGALTVTPDSSGVTSPHESSSGLSTASSSTPPTTLPAVVKKEKDTGFIQLCTPGVIKQEKTSGGYCQMGATSPISICGVSTSGGQSFHFGINSRNNEALQQKDQKPVSGLYLPVTTIGGAWSRSPSVGDNSPIHRVGELFTSSPTFSSFTRQEGGTAASMTQGKSGTHKICLVCSDEASGCHYGVLTCGSCKVFFKRAVEGQHNYLCAGRNDCIIDKIRRKNCPACRFRKCLMAGMNLEARKTKKLNRLKGCQNSAPPELTPELPVETRSIVPKCMPQLVPTMLSLLKAIEPDTIYAGYDSTLPDTSTRLMTTLNRLGGRQVISAVKWAKALPGFRNLHLDDQMTLLQCSWLFLMSFSLGWRSYQQCNGNMLCFAPDLVINEERMKLPYMAEQFEQMMKISSEFVRLQVSHDEYLCMKVLLLLSTVPKDGLKSQAVFEEIRMSYIKELGKAIVKREENSSQNWQRFYQLTKLLDSMHEVQRRSALVERFRRPWQHRNSASDH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that contributes to sodium ion homeostasis, proton export across plasma membrane, circadian behavior, regulation of hematopoietic stem cell differentiation, calcium ion homeostasis by functioning in spindle, microtubule organizing center, nucleus, mitochondrion with DNA-binding transcription factor activity, zinc ion binding, nuclear glucocorticoid receptor activity, sequence-specific DNA binding, steroid binding.2. The designed protein must have at least one NR LBD, Nuclear receptor domain.3. A protein with Nuclear receptor capability for targeted gene regulation.4. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "LTAVAIKRLIKMAKKITAFRDMCQEDQVALLKGGCTEMMIMRSVMIYDDDRAAWKVPHTKENMGNIRTDLLKFAEGNIYEEHQKFITTFDEKWRMDENIILIMCAIVLFTSARSRVIHKDVIRLEQNSYYYLLRRYLESVYSGCEARNAFIKLIQKISDVERLR",
    "response#2": "PEQQNLLDYIMDSYSKQQIPQEVSKKLLHEEFSAEGNFLILTEMATSHVQVLVEFTKKLPGFQTLDHEDQIALLKGSAVEAMFLRSAEIFSRKLPTGHTVLLEERIRNSGISDEFITPMFNFYKSIGELKMTQEEYALLTAIVILSPDRQYIKDRESVERLQEPLLDILQKFCKLHHPDNPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCS",
    "response#3": "QQQSIIPYSMLTSTNQTPEAPEARPAASWVTWCGAQQPVALKNPQVVCEAAAAGLLKTLRFVKYLPCFQVLVLEQQLVLLRGCWASLLMLELAQDRLYFETAETSEPSRLQRSLTTRRRETEGFEPLLLSTSQPNLAPLSEAEQLPWVTTVRAIKTFLAKCWSLDISTKEYAYLKGIMLFNPVLPGLQCVKYIEGLQWGTQQILSDHVKMTNQGYQNRFTELNNTLFLLKFIDANVIAELFFRPIIGTVSMDDMILEMLCAR"
  },
  {
    "reference": "MNRMFDLVLGTGNAKKLVELRMMLPEETIALTALSEIENAIDVVEDGETFSANAAKKATEQAKHLERWVLAEDSGLSVDALKGAPGVYSARYAGTHGDDEANNEKLLRELTDVPMDRRGAQFNCHLCLSDPDGNVRLAESGICRGRIATERSGGAGFGYDPLFVIPEYHKTFGELNLTVKRALSHRSRALRLFIPQLLRLVQSNSTSA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein requires Mg(2+) to activate a critical catalytic site.2. The ideal protein would be one that plays a critical role in nucleotide metabolic process through nucleotide binding within cytoplasm, cytosol.3. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.. The designed protein sequence is ",
    "response#1": "MNIVKLL",
    "response#2": "GWAA",
    "response#3": "SMSPQTETKASVGFKAG"
  },
  {
    "reference": "MDARQMKIEAARAALAYVESGMRLGVGTGSTAEEFVRLLAEKVAAGLVIEGVPTSERTARLCVDLGVPLKSLDELPELDLTIDGADEVDGALRLIKGGGGALLREKIVAASSQRMIVIADESKVVQTLGAFALPIEVNPFGLVSTRIQIEKTAARLGLSGALNLRQSEDGNFMTDGGHFIIDASFGRIPDAEALSIALNSIPGVVEHGLFINMATLAIIAGPSGARTLQANNT",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate..2. The protein must have high specificity towards ribose-5-phosphate isomerase activity.3. The desired protein should be able to perform aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate The designed protein sequence is ",
    "response#1": "MF",
    "response#2": "MTGIR",
    "response#3": "LRTLGI"
  },
  {
    "reference": "ILNSFIGIAAVQIGLTDVLKTIGIEPDYIIGYSVGDFGCAYADGCLTAEEVILSAYNRGLALENKPYFKDSMADISIGYHQDVVRNFKRSRSEKWVCMPLLKVKCQNVDPSANSYTNGVETPELFEETARLIHANAITIEIAPHGLLQAILRRSLKKDVVNVALTQKAHPDNVQVLFTAFGRLYEAGLNPHLANIYPHVPFPVSQGTPMLAHLVEWEHSENWFVASFKDLDKIKIGERTVLMSMNDEFKYMTGYVINGCNLYPVTGYLFLVWETLGMMMGEPYTEVSVVFENVRFQRATNIPKEGNLVFTITIHKKTGNFEILESCASIVTGRIYAKKNVGQDIRALPPLPEASGPGIKHLLTKDFYKELRLRGYQYSGLFRGVIGCNVEGTRGRLSWVNNWVTFMDCMLQLKIIGQDTRGLFVPTRIKKLSIDAPMHCNAISKMNRASHNKSFEVRVYPNLNVIRAGGIEIRGLQTTSIVKRQPPEVPVYEKHSFIPNFGRYKMKIEDVLRTNVQLVFENIQSHIVKSIELVDEEYKKNDLKPILESIGSILADLPFAQADLLMISDEPLKVPSNITVENRKLVRESNTILFIGANLLGRPKVFQKAIGTLCDNAFLMSREMEHPSIKKYSDIDIVTCYDTGSEYLVLLRKRMEAKPAKFVRVSAADDTFSWVDEIKKELKEGQKLVLYAQDGPINGLLGLVNCLRKEPGGEIVYGLFISDPSAPPFNPDLEFYEEQLNKDLIFNVYKDGQWGTYRHLSLDLDTVRA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. We need a protein that can perform dodecanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxotetradecanoyl-   [ACP] + CO2 + holo-[ACP] with high efficiency.2. The protein must be able to palmitoyl-[acyl-carrier-protein] hydrolase activity, 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity.3. For general function, the protein need meet that This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.; Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH The designed protein sequence is ",
    "response#1": "IAVIGVGCRFPGGIGSPHELWDALMGGADLVSEVPQDRFNINKFFDSDPAKTGAVRNRYGGFVDNILSFDAEFFGLFPAHAARIDPQQRLALEATYHALEDAGVPIESVAGSRTSVFMGAFMYDHLCTQTATLQRDNISPHVAMGASTSGIANRVSHQFDLRGPSVTLDTACSGSLVALHLACQSIWTGEADGAIAGGVNAILRPESSIMMSKAGFLSPDGRCKSFDASANGYVRSEGVGVVYLKRLSKAMAEGDRIYAVVRGSAVNQDGYTPEGIMVPSRDAQVDMLKDAYTCAGLSPAAVSYVETHGPGTPVGDPVEAAALGEVLGGPGRQEPLWIGSVKGNLGHLEGASGIAGFIKAALVTYHGQVPAQLHHHHPNPAIPFARLGLGVPLKNTPLASRGRDTSEPLYVGVNSFGAGGTNAHVVLARLQYDWLPNLQSAMRNETKTLVVVENQPLSGVLGFVNCLRREPGGTNVKCVFVQNGNIKFNLSDPLFREQLDKDLAINVLNQDVWGSYRHLPGISHSGGELGCAYADKCLTIEQTILSAYFIGTICAEKTIIHSSMAIVNIDYEYLKNKCPADIEIVCHNSENSCVVCGSKESIEGFMKELQVNNIYVKEINCNIPFHSSYVASAERQLLLSLHKIIPHPKKRSSKWISTSIPRNEWFTSSSKLSSAVYHSRSILNTVLFHQTTDLIPRNAVVIEIAPDDVLQHVLSESLHPNVTNLVLTRSTTQNVDIFLQPNPDLYGLLDGRLKVVDKPTPWEGGIVGINSFGFGGANAHVILKSNPKPKVITPTVGPPKLILSSGRTFDAVQEFLEDADQHKDDDEYLTLINDIHSRPISGHFYRGFS",
    "response#2": "MSPHPYDLILAILMRRYVPHLGTAKGYARSEAVVSIFLQKSDVARRKYATIIHIKTNTDGYKDNGITYPSVEGQRLLLQSVYQECKLSPSQISYIETHGTGTVVGDPVEVSAIADVFCPGREEPLWIGSVKSNMGHAEPAAGLCGLIKVLICIENSLLAPNLHFHKPIPSIPALNNGQIRIPTYSMPWKADYAGISAFGFGGVNTHVVLKGDEVVISGVAGCFPDSGNMYQFRHNLMNKIDMVSDDDRRWTLDHPEIPQRTGKLLHVNKFDASFFGVHYKQAQTMDPMCRIMMEKAFEAIVDAGVQPRQLRGTKTGVFTGACFSESEKTWFYEKMQVNGFGITGCSRAMLANRMSYWLGINGPSYAVDSACSSSMYALEHAYKSIRDGHCDSALVSGCNLCLHPYVSLQFSRLGVLSPQGMCKVFDRDANGYARSEAVCVMFLQKARNAKRIYATVLHSKTNCDGYKEQGITYPSGEMQRVLLEDFYFECNVDPATVDYVEAHGTGTKVGDPEELTALDHVFCKGRKEPLFIGSIKSNIGHSEPASGMCSISKVILAMETGVIPPNLHYNNPREGVPALAEGRLKVVTENQPHDCKRVGINSFGFGGANAHVLLQSPHPYDLILAILMRRYVPHLGTAKGYARSEAVVSIFLQKSDVARRKYATIIHIKTNTDGYKDNGITYPSVEGQRLLLQSVYQECKLSPSQISYIETHGTGTVVGDPVEVSAIADVFCPGREEPLWIGSVKSNMGHAEPAAGLCGLIKVLICIENSLLAPNLHFHKPIPSIPALNNGQIRIPTYSMPWKADYAGISAFGFGGVNTHVVLKS",
    "response#3": "DNDIVITGFSGRLPESQNIDEFKNNLFDGIDMVTDDERRWPRGLHGLPARTGKLKDLASFDATFFGVHAKQANVMDPQLRMLLELTYEAIIDAGINPTEIRGSKTGVFIGVSDSESDEFFTADPDMINGYGLTGCCRAMFPNRISYTFDLTGPSFAVDTACSSSLYAMHQAIMAMRSGLCDAAIVGGVNLVLKPTSSLQFHRLNMLSPDGKCKAFDAMGNGYVRSEAAVVIYLQKARDAKRIYATVVNSKVNDDGYKVQGITFPSGEMQNKLMREVYAESGIDPNEVVYIEAHGTGTKVGDPQEVNSIADLFCSKDRRSPLLIGSVKSNMGHSEPASGLCAIAKILIAMEAGVIPANLHFDIPNPDIPALNDGRIQVVDKSTKWKGGLVAINSFGFGGANAHVILRSPHPYDLILAILMRRYVPHLGTAKGYARSEAVVSIFLQKSDVARRKYATIIHIKTNTDGYKDNGITYPSVEGQRLLLQSVYQECKLSPSQISYIETHGTGTVVGDPVEVSAIADVFCPGREEPLWIGSVKSNMGHAEPAAGLCGLIKVLICIENSLLAPNLHFHKPIPSIPALNNGQIRIPTYSMPWKADYAGISAFGFGGVNTHVVLKSS"
  },
  {
    "reference": "MFDSIIIGQYVPGDSVVHRLDPRVKLTAVFIFLVFMFMTRDPLLLTVAVLLSFGGLLASRVPLSFYAKGMRFISIIIVLTFVLHLFMTGGGEVIVELPFATIYSGGLIEGFMLAMKLAMIITIASLLTLTTTPIDLTDGMERMLAPFKRVKLPTHELALMMSIALRFIPTLIEETRTIVLAQLARGTNFSEGSLWKRLKALIPILVPLFTQSFKRAEELATAMEARGYAGGEGRTKYRQLHWGLKDSVVLAVFLLFAAAVMAERFMGG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates..2. The protein should be optimized for plasma membrane-specific folding and stability, while maintaining transmembrane transporter activity.3. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "MLWNWVALVGGIISAVVFSYILQAAYFHELSLLSAVILGIVLIAALALRAFAGKKSVQASYFASTKVKHELRSLIYRKLASMPLNQVNQQSTSSIIQVASEGVEQLEIYFGRYLPQLFYSLLAPLTLFAFLIFFSFKTAIILLICVPLIPMSIIAVNKIAKKLLAKYWSIYVGLGSSFLDNLQGLITLKIYQDDAYKAKAMDKEAEHFRKITMKVLTMQLNSVSLMDLLAYGGAAIGILTALLQFQNAQLSVLGVILFILLSSEFFIPLRLLGSFFHVAMALVGGIISAVVFSYILQAAYFHELSLLSAVILGIVLIAALALRAFAGKKSVQASYFASTKVKHELRSLIYRKLASMPLNQVNQQSTSSIIQVASEGVEQLEIYFGRYLPQLFYSLLAPLTLFAFLIFFSFKTAIILLICVPLIPMSIIAVNKIAKKLLAKYWSIYVGLGSSFLDNLQGLITLKIYQDDAYKAKAMDKEAEHFRKITMKVLTMQLNSVSLMDLLAYGGAAIGILTALLQFQNAQLSVLGVILFILLSSEFLGA",
    "response#2": "MLWNWVALVGGIISAVVFSYILQAAYFHELSLLSAVILGIVLIAALALRAFAGKKSVQASYFASTKVKHELRSLIYRKLASMPLNQVNQQSTSSIIQVASEGVEQLEIYFGRYLPQLFYSLLAPLTLFAFLIFFSFKTAIILLICVPLIPMSIIAVNKIAKKLLAKYWSIYVGLGSSFLDNLQGLITLKIYQDDAYKAKAMDKEAEHFRKITMKVLTMQLNSVSLMDLLAYGGAAIGILTALLQFQNAQLSVLGVILFILLSSEFFIPLRLLGSFFHVAMISLLQ",
    "response#3": "MLWNWVALVGGIISAVVFSYILQAAYFHELSLLSAVILGIVLIAALALRAFAGKKSVQASYFASTKVKHELRSLIYRKLASMPLNQVNQQSTSSIIQVASEGVEQLEIYFGRYLPQLFYSLLAPLTLFAFLIFFSFKTAIILLICVPLIPMSIIAVNKIAKKLLAKYWSIYVGLGSSFLDNLQGLITLKIYQDDAYKAKAMDKEAEHFRKITMKVLTMQLNSVSLMDLLAYGGAAIGILTALLQFQNAQLSVLGVILFILLSSEFFIPLRLLGSFFHVAMLFSNYFFN"
  },
  {
    "reference": "MARKKIALIGSGMIGGTLAHIIGLKELGDVVLFDIAEGIPQGKALDIAESSPVDGFDVSLTGANSYDVIEGADVVIVTAGVARKPGMSRDDLLGINLKVMEQVGAGIKKYASSAFVICITNPLDAMVWALQKFSGLPTQKVVGMAGILDSARFRHFLSEEFKISVKDVTAFVLGGHGDSMVPLVRYSTVGGISLPDLVKMGWTTQEKIDQIIQRTRDGGAEIVSLLKTGSAFYAPAASAVSMAEAYLKDTKRVVPVAAYLSGQYGVKDTYVGVPVVIGAGGVERVIEIDLDKEEKAAFEKSVSAVQKLCEACIAVAPGLK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a high affinity for NAD(+).2. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.3. The protein design must be focused on enhancing the efficiency of tricarboxylic acid cycle, carboxylic acid metabolic process.4. The protein must exhibit the following characteristics: Catalyzes the reversible oxidation of malate to oxaloacetate.. The designed protein sequence is ",
    "response#1": "LDTARYRAFLADEIGCSPKEIQGMLLGGHGDTMVPLPRYTTVGGIPVTELVEADKLNAIIERTKNGGGELVKLMGTSAWYAPGAAAAQMVEAIVKDQKKIIPVCIKLEGEYGIDDCYLGVPAVIGKNGIEKVIELKLNAEEKALMETSRKHVKEVMG",
    "response#2": "TMRLRAYIAKKLKIPVYRVNGFVGGEHGEDAVVLWSTVTVNGKPFSEDLGVTKAEVEDYVKKIPGEIIRVMGGTTWGPGTIIAELIRAVALNENKVMSIATPRQFEDEIIHVSVPTVVGSSIGPSLENLLDEKDRWNLMAG",
    "response#3": "TTLDTIRSEKFIVQAKNIEINRNDISVIGGHSGVTILPLLSQIPHVEFTEQELKDLTHRIQNAGTEVVEAKAGAGSATLSMAYAAMRFVVSMARALNGEVITECAYIEGDGKFARFFAQPVRLGKNGVEEILPLGTLSAFEQQALEAMLPTLQTDIDNGVKFQ"
  },
  {
    "reference": "MWQFRIFLLFASTWGISGVSAHPDPVFSSSEGAHQVLRVRRANSFLEEVRAGSLERECMEEICDFEEAQEIFQNVEDTLAFWIKYFDGDQCSTPPLDHQCDSPCCGHGTCIDGLGGFSCSCDKGWEGRFCQQEMGFQDCRVKNGGCYHYCLEETRGRRCRCAPGYELADDHMHCRPTVNFPCGKLWKRTDKKRKNFKRDIDPEDEELELGPRIVNGTLTKQGDSPWQAILLDSKKKLACGGVLIHTSWVLTAAHCLESSKKLTVRLGEYDLRRRDPWELDLDIKEVLVHPNYTRSNSDNDIALLRLSQPATLSKTIVPICLPNSGLAQELSQAGQETVVTGWGYQSDKVKDGRRNRTFILTFIRIPLAARNDCMQVMNNVVSENMLCAGIIGDTRDACDGDSGGPMVVFFRGTWFLVGLVSWGEGCGHLNNYGVYTKVGSYLKWIHSYIGERDVSLKSPKL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should contain one or more Peptidase S1 domains that are essential for its function.2. For general function, the protein need meet that Exerts a protective effect on the endothelial cell barrier function.3. Ensure the presence of a signal peptide within the protein.4. The designed protein should be involved in regulation of circulating fibrinogen levels, positive regulation of establishment of endothelial barrier by localizing to serine-type endopeptidase complex and exhibiting oligopeptidase activity, calcium ion binding. The designed protein sequence is ",
    "response#1": "IVNGTLTKQGDSPWQAILLDSKKKLACGGVLIHTSWVLTAAHCVEGTKKLTVRLGEYDLRRRDHWELDLDIKEILVHPNYTRSSSDNDIALLRLAQPATLSKTIVPICLPNNGLAQELTQAGQETVVTGWGYQSDRIKDGRRNRTFILTFIRIPLVARNECVEVMKNVVSENMLCAGIIGDTRDACDGDSGGPMVVFFRGTWFLVGLVSWGEGCGHTNNYGIYTKVGSYLKWIHSYIR",
    "response#2": "TKPVGPWQDCAEARQAGHEQSGVYELRVGRHVVSVWCEQQLEGGGWTVIQRRQDGSVNFFTTWQHYKAGFGRPDGEYWLGLEPVYQLTSRGDHELLVLLEDWGGRGARAHYDGFSLEPESDHYRLRLGQYHGDAGDSLSWHNDKPFSTVDRDRDSYSGNCALYQRGGWWYHACAHSNLNGVWHHGGHYRSRYQDGVYWAEFRGGAYSLRKAAMLIRPLK",
    "response#3": "IVGGCVSKPHSWPWQVSLRRSSRHFCGGTLISPKWVLTAAHCLDNILALSFYKVILGAHNEKVREQSVQEIPVSRLFREPSQADIALLKLSRPAIITKEVIPACLPPPNYMVAARTECYITGWGETQGTFGEGLLKEAHLPVIENKVCNRNEYLDGRVKPTELCAGHLIGGTDSCQGDSGGPLVQ"
  },
  {
    "reference": "MYVGSSGLLKKYDEYLLEHGYGIEELVAKAADCLFKHFKDYQSIAFLVGPGNNGADALCTGLMLHHIKKQVVFYYCGEPDNFSKANQYYYDKCLDENLTLIYLNDHTIYEFAEDLQNYDCIGDGFFGFGLHSSPRGIYADVIELINSRYNKDILAIDIPTGLNCNTGYPYGAVLYATKTISLTALKEGYLNPDSQSFCGEIVLETLDVENVFMEAGLFEMVDDDYVHLHLKERKFDGYKNYYGTDLIIAGSDEYKGAPLLAAKACLNSGAGIVKVMSVDSVIKILPLSIPEAIGIKIHRLSETDFEGYQAVMIGSGLGLDLDAYNYLLDTLQYTTSPLIIDGDGLTILSNNLDLLKKQKKPVILTPHLGEFKRLTDYNDQDDIMEKAVEFAREYESILVLKGPHTIVTNGYESYRMTSGNKAMAVGGMGDTLAGMITAFLGQKYDPLTAVILAVYIHGFTGDMLAKKRYTVLPSKLSDMIPSAMFVLSQNYKIID",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein with NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, ATP binding is needed to enhance nicotinamide nucleotide metabolic process in a specific context.2. The designed protein should have a binding site that is specific to the target (6S)-NADPHX, AMP ligand.3. The desired protein should be able to perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate4. The presence of Mg(2+) is necessary for the protein to function in the desired environment.5. The protein contains novel YjeF N-terminal domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MQVAELNSEYLGVTNFQLMEAVGAKAASEIIARMPKGKPNPKVHVFSGPGKNGGDGFSIARHLTSMGLRVKVTLVSRTAEVNDYAARQQLTAILQMKESVEFEAAHDSSQLKAIEAELIVDSLLGTGVKGPLREPLLGAVRALNKSRGFKVAVDIPSGLDTDTGEPHGDAVKADLTICLHKTKKGLLEAKQYVGTLVVLPIGIGAGSGDVLTGIRSTHKGVRGKTLILAGSPGLLGAAILAARACLAAGSGLVTLGIPASLNAPVKAAVPEAMTLPLPETSDGSIARAALARILEHSSGADAVAIGPGLGSHPETQEVVVECLAKCDRPMALDADGLNALAARGAQAVLRERRGPTVLTPHPGEVARMLGVDKRAVVEDPRVHVERASEQANAIVVLKGPTSFICSGDGHLYLNHYPNDGMATAGSGDVLAGMIGGIVGQQKIDPKDAVCLAVYVHSLAGDLASRRLGHRAMTAVDIL",
    "response#2": "MRLLEKRALESGTSFGDLMHNAGRAVASEICGLFDSFPGKKALVLVGPGNNGGDGLVAARFLNDAGAITHVYLLAPRDPGDLVYNEALEAGISPIEVESDAGFSKLRELLGEADVVVDAIFGTGLGRPIGGASAAALTLVAEARNQRPDMVVIALDLPSGLNADSGEIDDSTLAVDYTITLGYAKRGFFLFPGADYTGQILVADIGIPDGLDGRGDLLPTLQSRGKRFHKGQFGRMLVLGGSKNYTGAPSFSSLTGINFGLDLVITYTPQIIGDVIRSYSPNLIVRSQPGDWLSKQNWEEIIWLVEWSNAILIGPGMGEEKETEALLIDLLKKFREDKKAFVLDADALKLVKNHHDLLKRQSCILTPHEGELKIMTGAELPPYDDIEARSESIIKLAKELNCVLLVKGPYDYISDGEELRINRTGCQEMAVGGTGDVLAGLCASFLATGNKIFESACSAAFLNGYIGEFCKQKVGTRFNAMDMIDKINEAISEVC",
    "response#3": "MQVAELNSEYLGVTNFQLMEAVGAKAASEIIARMPKGKPNPKVHVFSGPGKNGGDGFSIARHLTSMGLRVKVTLVSRTAEVNDYAARQQLTAILQMKESVEFEAAHDSSQLKAIEAELIVDSLLGTGVKGPLREPLLGAVRALNKSRGFKVAVDIPSGLDTDTGEPHGDAVKADLTICLHKTKKGLLEAKQYVGTLVVLPIGIPLEAEGRGDLLPTLQSRGKRFHKGQFGRMLVLGGSKNYTGAPSFSSLTGINFGLDLVITYTPQIIGDVIRSYSPNLIVRSQPGDWLSKQNWEEIIWLVEWSNAILIGPGMGEEKETEALLIDLLKKFREDKKAFVLDADALKLVKNHHDLLKRQSCILTPHEGELKIMTGAELPPYDDIEARSESIIKLAKELNCVLLVKGPYDYISDGEELRINRTGCQEMAVGGTGDVLAGLCASFLATGNKIFESACSAAFLNGYIGEFCKQKVGTRFNAMDMIDKINEAISES"
  },
  {
    "reference": "MTKKRKLEGEESNEAEEPSQKLKQTPEEEQQLVIKNQDNQGDVEEVEYEEVEEEQEEEVEDDDDEDDGDENEDQTDGNRIEAAATSGSGNQEDDDDEPIQDLLEPFSKEQVLSLLKEAAEKHVDVANRIREVADEDPVHRKIFVHGLGWDTKTETLIEAFKQYGEIEDCKAVFDKISGKSKGYGFILYKSRSGARNALKQPQKKIGSRMTACQLASKGPVFGGAPIAAAAVSAPAQHSNSEHTQKKIYVSNVGAELDPQKLLMFFSKFGEIEEGPLGLDKYTGRPKGFCLFVYKSSESAKRALEEPHKTFEGHILHCQKAIDGPKPGKQQQHHHNPHAYNNPRYQRNDNNGYGPPGGHGHLMAGNPAGMGGPTAQVINPAIGQALTALLASQGAGLAFNPAIGQALLGSLGTAAGVNPGNGVGMPTGYGTQAMAPGTMPGYGTQPGLQGGYQTPQPGQGGTSRGQHGVGPYGTPYMGH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The ideal protein would be one that plays a critical role in mRNA stabilization, cell death, abscisic acid-activated signaling pathway, ethylene biosynthetic process, defense response through mRNA binding, mRNA 3'-UTR AU-rich region binding within nucleus.2. The protein must exhibit the following characteristics: May function in complex with UBP1 and contribute to the stabilization of mRNAs in the nucleus; Heterogeneous nuclear ribonucleoprotein (hnRNP)-like protein that acts as component of a complex regulating the turnover of mRNAs in the nucleus; However, unlike UBP1, UBA2A does not stimulate pre-mRNA splicing.; Binds with high affinity to RNA molecules that contain U- rich sequences in 3'-UTRs.3. Prioritize Acidic residues, Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.4. The protein contains novel RRM domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "AMATPVTTMTFDTAQELLWTGNDYGRVTSFYGSELQRYTSFKAHTTPDGPVRQILVNDKGVVVLGSRDIHMASRKGPPIWHIRHDDMKDLRCMSFTSKGTAEILAAGLQDTMFVIDLAKGEVVKQVPTAHSYKVMKRSRYICAATKDGTVDLIDPVNFSVVKSWNAHSALISDMDAQHDFIVTCGYSLRQGQNYMLDPFLNVFDIKKMSSMSPIPFPAGAAFVRMHPRMSTTSIVMSQSGQMHVVDLMNPNTSNVRQANTMTYLTMFEIAPSGEAVALADAEFNIHIAGLETQIPNAYCNGMLQVLYFIEPLRCAMKSHLCEREFCLACELGFLFHMLDEQKGQTCQANNFLRAFRTIPEASALELIIGDHDQLQGKANFTRLIQNWQRFLHQQVHSEYCVRGTIYQNAIPEGAIPVEGCQQEIADYYSIPVYTEPCPIQENIPPGANILDQSSEAVPESPPKSEPEVSIVSQLFGIEMETLLRCRCGHETHRTSYPTLTNLSYPSSNTDEPPQVISFSNILLHSLTSEQVMQAWCSVCSRYQTHVQKKLIKSLPDAGLEPHIPNAYCNCMIQVLYFLEPIRCLVQNHLCQKEFCLACELGFLFHMLDLSRGDPCQASNFLRAFRTIPEASALGLILADSDEQTGKARLGRLIQSWNRFILTQLHQETQEQEGPQAYRGASASAPGLSGESVIGRLFGCEVENSSLCRCGKETVRSTLTLLFTMHYPELGSLEKAVKERDFAEVLKKSICLEQSTQAWCENCEKYQPTVQTRNIRGLPDVLVINCEVNSVKEAEFWKAQAEVRRPKGPRGTSADERCGT",
    "response#2": "NPMQKSNTFGYPTTCYKAVNTKDGLTYCLRRVHGFRLSNTKCMSIIDMWKKMYHPNIVQLREVFTTKAFGDNSIVFVYDFFPGAETVLLKHFSGPGGMNGFNNSFNMEGGGGGGSRSYNSGNKGLNGPRQHAGLLPESMIWAYVVQLSSALRAIHAAGLAFRTMDPSKILIYSKSRIRLNCVGILDVLTFDSQGNPLAAIQHYQQDDLMYLGKVVLALACNTVMAIKRDNFQNSMELIARSYSADLKNFFLYLLTNQSRPRSINDIMPMIGARFYTQLDSAQLRSDVIENELTKEVENGRLFRLLAKLGTINERPEYNLDMQWSETGDRYILPKTVESCLQNPRKEVSLPMQKSIHSHAHFFCSQENKQSLDFDQGLKVLILYLLIRIKGINNTFNQIEQRKERTLSKLELFHESLTHDKEKYKEQSKKEVHITSAQIYESWNNSERDLSSFFEEINAKFQEIKIKLAEVNPMTAAIQKIPKQRPEDLQVFVKKSIQGLEDLRRIPIEGRKEKDTVLDVAQNAVRCLKQRDRNLQEHFNKKDTLMQALGKIRSAHMNLEALSSNLDLFTSQLSKSQRKRQSDLWKLLAAAPTPSPPPILPKTVESCLQNPRKEVSLPMQKSIHSHAHFFCSQENKQSLDFDQGLKVLILYLLIRIKGINNTFNQIEQRKERTLSKLELFHESLTHDKEKYKEQSKKEVHITSAQIYESWNNSERDLSSFFEEINAKFQEIKIKLAEVNPMTAAIQKIPKQRPEDLQVFVKKSIQGLEDLRRIPIEGRKEKDTVLDVAQNAVRCLKQRDRNLQEHFNKKDTLMQALGKIRSAHMNLEALSSNLDLFTSQLSKSQRKRQSDLWKLLAA",
    "response#3": "LEGLFSELHSVAAEVGVPVAAAHFDMQEELLWMGNHRGHATSFFGPTMGRYSSFQVHLTDDVRHIQSMETGMLFLTKNNLKCHTRGGLVIFDYSMEEGADMHSLLLTDSNTLLMGGLQSCVVELDLNTVQETQKFSVEVPGVAIMRQSNRFFFCGHTSGKVSLRDLRTFKPEHEFDAFSGSLSDFDVHGNLLAACGFSSRGLNGLACDRFLMVYDLRMMRAITPLQVHVDPLFLRFIPTYTSRLAIISQSGQGQFCEPTGLASLADIFHVNTVGQLLMSFDVSSSKQALAFGDSGGGVHLWEPPQEQKQMLGERLYPLIHEMHPTLAGKITGMLLEIDNSELLHMLESPESLHSKVEEAVAVLQARAGLETQIPNAYCNGMLQVLYFIEPLRCAMKSHLCEREFCLACELGFLFHMLDEQKGQTCQANNFLRAFRTIPEASALELIIGDHDQLQGKANFTRLIQNWQRFLHQQVHSEYCVRGTIYQNAIPEGAIPVEGCQQEIADYYSIPVYTEPCPIQENIPPGANILDQSSEAVPESPPKSEPEVSIVSQLFGIEMETLLRCRCGHETHRTSYPTLTNLSYPSSNTDEPPQVISFSNILLHSLTSEQVMQAWCSVCSRYQTHVQKKLIKSLPDEPL"
  },
  {
    "reference": "MSVTATSHDILKSIDDSILPPEDTLSEAMYNNVKLDHYFNKLPKSSEENNINDQTNQLIVSAAPESEDSPAAEWKLLRTLAGAHQGWIRAIALDEITNKWYVTGSADSTIKIWDFENNSLKAVLTGHVLGIRSLCISKRHPYLFSGGEDKSLRCWDLERSNSDAGCQIRSYHGHLGGVYSIGLHPELDVLFSGGKDCVVRVWDIRSRVEAMTLLGHTNDITSIETDYNDPQVITSSMDGTIRLWDLRKSKTELLITNHSKSIRSMKSHPKEATFVSGDSNGEIKQWLLPKGELLNEFGTSQLSPNQRDNSRIINTLAINPVTNTLFSGYDDGKLEFYNYTTGNLQQSGQSPSLAGPEQSAIYASTFDMSGLRLLTCHGDKSIRIWGTSY",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be optimized for stability and solubility when expressed in catalytic step 2 spliceosome, Prp19 complex where it will be involved in mRNA cis splicing, via spliceosome, mRNA splicing, via spliceosome. The designed protein sequence is ",
    "response#1": "TPIDQFGMALLRGMGWKEGMGAGKGGKGPQQAAEPRKRAALLGLGAAV",
    "response#2": "PRKSVLEDDFEEEEEKEENEGIADGSEDEMDQRRKSPERERERDRDRRRDSHRHRDRDYDRDYDMDRDHDRDYERERGHGRDRDRERDRDR",
    "response#3": "PRKSVLEDDFEEEEEKEENEGIADGSEDEMDQRRKSPERERERDRDRRRDSHRHRDRDYDRDYDMDRDHDRDYERERGHGRDRDRERDRDR"
  },
  {
    "reference": "MIMWQSLILISSGAALGASLRWGMGLILNPLFAAFSFGTLIANYLGCFIIGLIMAMIWQHPQFSGEWRLFMITGFLGSLTTFSSFSAEVMENFIQQKWLIGLGIMSAHLFGCLIFTGIGVLITRWLN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be expressed specifically in the plasma membrane The designed protein sequence is ",
    "response#1": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPQ",
    "response#2": "KPYLTVKATGYQWYWGYEYPDQKISEITSVPLKQEEAVAKGVPYLLAADKPMVVPVNQVIRVQVTGADVIHAFALPAFGLKTDAIPGRLNETWFKADKIGVYYGQCSELCGVDHSNMPIEIHVVSQADFDAWVKL",
    "response#3": "AFHDHILMPIITAITLLVLGLLIWIVIRYNAKANPVPAKFSHNTTVEIIWTIGPVLILMVIAIFSFRLLFAYNDMPKPYLTVKATGYQWYWGYEYPDQKISEITSVPLKQEEAVAKGVPYLLAADKPMVVPVNQVIRVQVTGADVIHAFALPAFGLKTDAIPGRLNETWFKADKIGVYYGQCSELCGVDHSNMPIEIHVVSQADFDAWM"
  },
  {
    "reference": "MSQIKTTSTQAFEFDSIDAALADLKAGRVIVVVDDENRENEGDLICAAQFATPDTINFMAVEARGLICLAMTGDRLDELDLPLMVSNITDPNQTAFTVSIDAGSHLGVTTGISAEDRARTIQVAINPSTKASDLRRPGHIFPIRAREGGVLKRAGHTEAAVDLSRLAGLYPAGVICEIQNPDGSMARLPQLIQYAKHHKLKIISIADLISYRLKHDRIVIRETVADLPTQFGLFQIYAYRHTLDNTEHVAIVKGDPSQFDDHPVMVRMHSECLTGDALGSLRCDCRMQLQAALKMIENAGQGVVVYLRQEGRGIGLINKLKAYSLQDMGLDTVEANERLGFPADLRDYGMGAQILMDLGVHKIRLITNNPRKIAGLKGYNLEVVDRVPLLIEANDYNSYYLTTKAKKLGHMLLQTYLVTMAIHWEDDPQAVTERYERLEKLRHLAKSHDLLLQEEARPLAIALFDKPSLTVHLGFDQPRLATHDWYKQKNHPYVQAIGQILDYLVSLPYVQKLEFLISSGVDPLSNLQVQLDRQTFSAETSPSSICCEQLETQKIYSFNL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must bind to Zn(2+) in order to perform its enzymatic function.2. For general function, the protein need meet that Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.3. The designed protein have GTP cyclohydrolase II domains with specific sequence motifs that are crucial for its activity.4. A protein that is capable of catalyzing reactions within the 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4, Cofactor biosynthesis, riboflavin biosynthesis pathway with high efficiency and specificity.5. The protein design must be able to perform GTP binding, manganese ion binding, GTP cyclohydrolase II activity, magnesium ion binding in order to contribute to riboflavin biosynthetic process.6. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.7. The Proton acceptor; for GTP cyclohydrolase activity, Nucleophile; for GTP cyclohydrolase activity of the protein must be engineered to enable effective catalysis of GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate. The designed protein sequence is ",
    "response#1": "SSAIPTQLGDFRAHAYRSTFDGTEHLALVMGDVASAGQSEQGVLVRVHSECLTGDVMGSLRCDCGTQLAQSQADIANEGCGVLVYLRGHEGRGIGLVNKIRAYGLQDKGLDTVDANTAQGLPADARTYDVAAQILTDLGVRRIRLISNNLEKYRSLNNSAVHVVERRDYRRLPEFPREAVAKMPTKYGNFNIVGYQNKTTGEHHVALTMGDVGNGEPVLCRVHSECLTGDVFGSKRCDCGQQLEKAMEMVAKEGRGIILYLRQEGRGIGLINKLKAYELQEQGLDTVDANLKLGFPADMRSYGMGAQILADLGAKKLRIMTNNPAKITGLSGYGIEIVERVATTKLPTKQYGTVTVHAYESEIWPQPAIAMVWGDLSQGPPPLVRVHSSCVTGDLLSSLRCDCGDQLHAAMDAMAQEGRGALIYLEQEGRGIGLVAKMKAYQLQESGLDTVEANQKLGFKPDLRDYGIGAQIMADLGLKKIRFMTNNPAKVRGIDGYNIEIVDWVPKPMGRSPATTKLPTKQYGTVTVHAYESEIWPQPAIAMVWGDLSQGPPPLVRVHSSCVTGDLLSSLRCDCGDQLHAAMDAMAQEGRGALIYLEQEGRGIGLVAKMKAYQLQESGLDTVEANQKLGFKPDLRDYGIGAQIMADLGLKKIRFMTNNPAKVRGIDGYNIEIVDWVPPPKGRSPTVRKETVKLPTEFGIFDLHCFVAKNDGREHLALVYGEVAGQENVLTRVHSECLTGDVFHSRRCDCGQQLAAAMRQVVANGSGVVVYMRQEGRGIGLVNKLHAYRLQEKGLDTVDANIKLGFPPDLREYGIGAQILANLGVKSIRLLTNNPKKIVGLQGYGLQVTGR",
    "response#2": "MKLEVSTRLPTDYGYFRVLGYRHQIDGTAHVALVAGDVDKLRGAEDVLVRVHSECLTGDVFGSRRCDCGSQLQASMREIQENGSGVIIYLRGQEGRGIGLLKKLEAYRLQDEGLDTVDANLEQGLPEDAREYSVAGQILRDLGIKSVNLLTNNPHKGEGLVGFGVPYKERIGEVSTRLPTDYGYFRVLGYRHQIDGTAHVALVAGDVDKLRGAEDVLVRVHSECLTGDVFGSRRCDCGSQLQASMREIQENGSGVIIYLRGQEGRGIGLLKKLEAYRLQDEGLDTVDANLEQGLPEDAREYSVAGQILRDLGIKSVNLLTNNPHKGEGLVGFGVPYKERIGNATTKLPTKQYGTVTVHAYESEIWPQPAIAMVWGDLSQGPPPLVRVHSSCVTGDLLSSLRCDCGDQLHAAMDAMAQEGRGALIYLEQEGRGIGLVAKMKAYQLQESGLDTVEANQKLGFKPDLRDYGIGAQIMADLGLKKIRFMTNNPAKVRGIDGYNIEIVDWVPYKERIGN",
    "response#3": "MPEVSTRLPTDYGYFRVLGYRHQIDGTAHVALVAGDVDKLRGAEDVLVRVHSECLTGDVFGSRRCDCGSQLQASMREIQENGSGVIIYLRGQEGRGIGLLKKLEAYRLQDEGLDTVDANLEQGLPEDAREYSVAGQILRDLGIKSVNLLTNNPHKGEGLVGFGVAMDPNERRVAEVSLPTEYGEFQLIAYDNDIDSYQHIALVKGDVAGREDVLVRVHSECFTGDILGSLRCDCGAQLHRAMTTIDEAGLGVVLYLRQEGRGIGLFNKLRAYELQERGLDTHEANLHLGFDADPREYGIGAQILYDLGVKSMRLMTNNPVKRAGIEGYGLKVSGRVREPSGELGHGDEIIEVSTRLPTDYGYFRVLGYRHQIDGTAHVALVAGDVDKLRGAEDVLVRVHSECLTGDVFGSRRCDCGSQLQASMREIQENGSGVIIYLRGQEGRGIGLLKKLEAYRLQDEGLDTVDANLEQGLPEDAREYSVAGQILRDLGIKSVNLLTNNPHKGEGLVGFGVAMNPDELVSGPVASALLPTAFGDFVAVGFRSEIDGIEHIALVRGEIGAGDDVLVRVHSECLTGDVFGSLRCDCGPQLQAALRRIAEEGRGVVLYIRGHEGRGIGLLHKLQAYELQDNGRDTVDANLELGLPADARDYGTGAQILADLGIRSMRLLTNNPTKRAGLEGYGLSIVEPVRS"
  },
  {
    "reference": "MIRLLNNFNFSEIDNADPAQWCRLFQEAAAEFDVLLSDAQLNRFLMYYRELKFWNSRINLIASAESVTDIVIKHFLDSLTLIPCIPFPDGRLIDIGTGGGFPGIPLKIALNSLKVTLLEASRKKVSFLKSLRRVLNLQDMKILNERVEDLITQAPCPNRFDMVVSRAALKLPEYLRFGKELVSSHGVIIAMKGANYQHELEDVNDILEEYGIFLAEVRSLALPCTGDFRAILIFRKSLSRT",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must be able to perform rRNA (guanine-N7-)-methyltransferase activity under specific conditions.2. We are looking for a protein with broad substrate specificity for guanosine(527) in 16S rRNA + S-adenosyl-L-methionine = N(7)-   methylguanosine(527) in 16S rRNA + S-adenosyl-L-homocysteine.3. The S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MGKIIGFVLGGAIGSTARFMISRF",
    "response#2": "MSIALVTGASRGIGKAIAENLSSQGNKVIVHYNSSKEGAEETVKSIESSGGQAIAIQADVSKKENIDSLFNQVKLEWGRIDVLVNNAGITKDNLLLRMSEEEWDSVIDTNLKGVFLVTKAATKSMIKQRWGRIINIGSVNGVVGKPGQANYAAAKGGVISLTQSLAKELASRNITVNAVAPGFIETDMTNELSEEFKKQVLSQIPMKRFGKPEEVANLVSFLASEKASYITGEVLTIGGGW",
    "response#3": "MDW"
  },
  {
    "reference": "MISMLSENEAKILFFLKDLKKTNSVELAIKMGIPESSVLSLIELLREKGYVKTEVKSEKHYVLTEEGRKRKESGLPEDILINTLNGQEKDLNEIKNILNKDFNIAISWAKRKGLIDIKEGKVIPKVKTYMSSEYLALSNLERADSNTINLLKKRGLIEEKERKIVSVELIKEPKESEIGISNLNRELIISGEWKKYKLKKYNVEAFPPYYTISKKHYFREFLEKVKDIMISLGFKEINTGYIEMEFYNFDLLFQPQDHPAREIHDSFSVEGSGKIEDKDLLNNVKEIHEKFWKYEWKQDITLRLMLRSQTTATTARVLASRPKAPQKVFTLGKVFRPDAIDATHLIEFHQLDGVIIDNNFTFKELLGVLKEIFYRLGIKEVKFKPAYFPFTEPSVEAYGYLEKLGWVEMCGAGLLRPEILSSVGIDSTAGAWGIGIERLAMSFLNISDIRLLYSNNIEYIRDTKVKIE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein have enhanced ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) +   L-phenylalanyl-tRNA(Phe).2. The designed protein should be able to compete with endogenous proteins or inhibitors for L-phenylalanine, Mg(2+) binding.3. The designed protein should be involved in phenylalanyl-tRNA aminoacylation by localizing to cytoplasm and exhibiting magnesium ion binding, ATP binding, phenylalanine-tRNA ligase activity. The designed protein sequence is ",
    "response#1": "YMTLLERKIRELGIGAGYVEISNFVLIKDEKLFSNKYVKILNPVTDEYNAVRNSLIPGLLDFLSKNQHAKFPIRVFETGDVVVYDSSTDTGFRNDKRAAYAIMDNKVSYEDIQAPIHYILKSLGLEVNYKEENNNIFIEGRSASIFYENEKMGVIGEVNPDVLIRFGIEYPAVIAELYISEVN",
    "response#2": "YMTLLERKIRELGIGAGYVEISNFVLIKDEKLFSNKYVKILNPVTDEYNAVRNSLIPGLLDFLSKNQHAKFPIRVFETGDVVVYDSSTDTGFRNDKRAAYAIMDNKVSYEDIQAPIHYILKSLGLEVNYKEENNNIFIEGRSASIFYENEKMGVIGEVNPDVLIRFGIEYPAVIAELYISEE",
    "response#3": "YMTLLERKIRELGIGAGYVEISNFVLIKDEKLFSNKYVKILNPVTDEYNAVRNSLIPGLLDFLSKNQHAKFPIRVFETGDVVVYDSSTDTGFRNDKRAAYAIMDNKVSYEDIQAPIHYILKSLGLEVNYKEENNNIFIEGRSASIFYENEKMGVIGEVNPDVLIRFGIEYPAVIAELYISENYGSYDYMTLLERKIRELGIGAGYVEISNFVLIKDEKLFSNKYVKILNPVTDEYNAVRNSLIPGLLDFLSKNQHAKFPIRVFETGDVVVYDSSTDTGFRNDKRAAYAIMDNKVSYEDIQAPIHYILKSLGLEVNYKEENNNIFIEGRSASIFYENEKMGVIGEVNPDVLIRFGIEYPAVIAELYNFDALGVMPECGHLHPLLKVRTQFRQIFLEMGFTEMPTNNFIESSFWNFDALFQPQQHPARDQHDTFFLQDPALATEFPMEYLERVKKVHSEGGYGSQGYKYDWSIHEAQKNILRTHTTAVSARMLYKLALQKDFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADRGLTLGNLMGVLKEFFHKLGITKLRFKPAYNPYTEPSMEVFSYHEGLKKWVEVGNSGLFRPELLLPMGLPEDVNVLGWGLSLERPTMIRYGIKNIRELVGHKVF"
  },
  {
    "reference": "MVTDQREHELDPNTPIDEGGRRRTAQPMDDRWPDVFANGFVCYALFIAIVAVVPPWFRYFARPDDVVSLVLVPVVPSLIYAALLLTLGVGLRRRLRAAWWIMVIWVLALPTVGRVVAIADGQFDVLSVLGLLVSAALLLLALRGRAQFRARRVPGSVTLAMALFLLGGAVILLAGSALVLWFGTAQNFADAAWYVFDAMLADVGRVGYESDATAPLWVRSLVGLAGAGVVLGAATVLFRPNQGDRTLGVADEARVRGLLRDFGDHDSLGYFATRRDKTVVWDTGDATIARAGVSYGVIGSVALASGNPVGDPRRWQVAIARWKEHARETGLSLAVIGAGHEGARAYTEAGLRMLELGDEAVVDMGAFSLTGPGMKSVRHSVHALERRGYTTRVVRHSRLDEDDFARLADAASKWRGDGGDERGFSMALGRLGDPLDGDCVLVEAHDAEGILRGFLSFVPWGRAGLSLDLMRRDPSADNGLVELMVTALSGRSATVGIGQVSLNFAMFREAFERGSEVGAGPVARLWRHALLVASKNWQLESLYRSNAKYLPQWQPRYVGFEFTSDLPRVGLAAGSAEGFLKAPSVSLFHGRGGAGEPLETGGQARAAAVLALVPAAPDVVAEALAVDHLPEQVRVRRRKLDRLREQGIDPYPAVAPRTHTLAQVREQAGDLPADTETSMVVSVAGRVLLKRDMGRLGFATLRDGTGDLQVMVDVGHLGEAQLSFWEHQIDLGDQVSVSGAVVTTKKGELSIRATSVMLTSKSLRPLPD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should contain one or more Aminoacyl-transfer RNA synthetases class-II family profile domains that are essential for its function.2. We are looking for a protein with broad substrate specificity for ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-   tRNA(Lys).3. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+) binding.4. The protein must exhibit the following characteristics: Catalyzes the production of L-lysyl-tRNA(Lys)transfer and the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge; LPG synthesis contributes to the resistance to cationic antimicrobial peptides (CAMPs) and likely protects M.tuberculosis against the CAMPs produced by competiting microorganisms (bacteriocins).5. The designed protein should have at least Helical transmembrane domains to ensure proper localization.6. The protein can be targeted to plasma membrane and perform magnesium ion binding, nucleic acid binding, lysine-tRNA ligase activity, lysyltransferase activity, ATP binding. The designed protein sequence is ",
    "response#1": "LPALVSRLFFVAGVLLILETLFPGVAWVGFLVDVCSTLLFPVDEGSIGFGVFLLLLGATLARRKRVGWVLALVIFGFFLITDLAVVTGLVASLFALQTDFSAVPVYARFAFNLAALGALTACLVHYRAEFTARRAPGSVRKALLTLLVGLGVTVGVAMTLVTVFPNELVGPRGRLAWILERLGRALFSSTDGDLVAQPVSSPPGWISTVVGLLLGLTLLAAVVMLAGGPKLRRLLAGDAARERVARTLGNAFALVALWSLVSWPWRHSATVEAVNDALGVLGLPAEPSLFVIAMLLVLAGAVRRRLRAAYVVVVVLVSLSLGFQVLVAADSWSGGTAPDTAGTGRFAGLWWGLRERPLFNVGCAAVTLAVLLLVAAAFRQFPARLAHGSRRTALVVLVAGLLASFGLTLALTVLMPGSLDGLLEEARWALRATFGQSTSPVDPTFDGRMGHQWIFGLAGLASAAALVLATLVRAAVPKVASRVLFAVAVLCALAAVSAAFGARLMPLRRLVDALLMPAPANLGYAVFLAMLAVGVNNRKRVAYWLLTGYFGLQLVTDAVLLLLRIENSPPAWAHVPIRLWWFLAGNLVVTVAVLAVLASARQQFFARVQRASLPKALATVVAMITAFSLIGWGLVAMFPGSLRRDGLYELGYAIRQVTGGALDLNVHRAGMAPGWINLLLGFFGAVTLLTALAVRIPRIAGTIVGLLATVSLLSTLIPPFRHLIRVPRMFVDDYLFPMPSTSLAWTVVLGLLAVSLSLRKRVAWWITVAYLSLYMVLNLLVVLIAASDPSWVDEWRESVIGLTFNIVLLVVLIAGYRQFNTRVRRGALLSALAVLVGGLAAGTLAGWALVSVAPGTLEPHERLPYAFNHVVAFATIDVKSFATHHAPWMVNWVLGLFGALALILAAIVL",
    "response#2": "LPALVSRLFFVAGVLLILETLFPGVAWVGFLVDVCSTLLFPVDEGSIGFGVFLLLLGATLARRKRVGWVLALVIFGFFLITDLAVVTGLVASLFALQTDFSAVPVYARFAFNLAALGALTACLVHYRAEFTARRAPGSVRKALLTLLVGLGVTVGVAMTLVTVFPNELVGPRGRLAWILERLGRALFSSTDGDLVAQPVSSPPGWISTVVGLLLGLTLLAAVVMRAAVPKVASRVLFAVAVLCALAAVSAAFGARLMPLRRLVDALLMPAPANLGYAVFLAMLAVGVNNRKRVAYWLLTGYFGLQLVTDAVLLLLRIENSPPAWAHVPIRLWWFLAGNLVVTVAVLAVLASARQQFFARVQRASLPKALATVVAMITAFSLIGWGLVAMFPGSLRRDGLYELGYAIRQVTGGALDLNVHRAGMAPGWINLLLGFFGAVTLLTALAVWRRSTTAQAADPETARLRRLLLEHGEHDSLGYFATRRDKSVLFSRDGRAALTYRVEGTVCVASADPVGARSGWPEVIGDWLERCRTHGWHAAVLSCSEEGARAYVAAGLRPFALGDEAIVELDSFSLSGRDMRPVRQAVTRVARAGYTARVRRHSELDPAELAELERLADAWRGEETERGFSMALNRVGDPADGRCVVVTAHDPQGQVRGFLSFVPWGTRGLSLDLMRRDRTAPNGLNEFLVSRLAEQGPGLGVRRVSLNFAVFRNVFAGADQVGAGPVTRLTDAALSFASRFYQLETLYRSNDKYRPQWVPRLLCARLDTGRGRLLARLADG",
    "response#3": "GLLDRYGQDDSLGYFATRRDKAVIFAPNGRAAVTYRVEVGVCLVSADPIGDKTAWSQAIAAWLQVCQAYGWAPAVMGASAEGALAYREHGLNALELGDEAILYPRTFSISGPHMATVRQAVNRARRSGLSVRIRRHRELTADEAKQIITNADLWRDGGTERGFSMALGRLGDSTDGDCLLVEAVDPDDNAVAMLSLVPWGANGVSLELMRRSRQSPNGTIEFMVTELLTRAEAMGITRVSLNFVMFRSVYADGSRIGAGPVLRLWRSVLMFASRFWQFEQLYRSNVKYQPEWVPRFACLHWVPAAAGWILGVIAGASLLSSVSSTIRHLTKVPREFIDHYLFNFPDTSFAWAFAVAILAGALVARKRIAWWMLLANLVVAVGFDVVALSARDGKRLEQVGEVLGLALHLAAIAVLLLSYKEFWAKVRRGAVFRAAAVLVVGNIIGILLAWGLLTVFPGSLQPGYRLPYAINRVSGFALATADLFVGKPHGFINALMGLFGALALMAAVVVLAV"
  },
  {
    "reference": "MKLISGTAHPALAEGIAEYLETPLTDVNVTAFPDGETYVKINENIRGQDVFIIQPTCPPTNHNLMEMLILVDAAKRASAERITAVIPFYGYARQDRKDQPRVPITAKLVANLISAAGVDRVLTMDLHAAQIQGFFDIPVDHLYAKPVLLRHLVEQRGQQDGSNLTVVSPDVGGVRMAKSYAAALGAELAIVAKNRVSATQVEPMRLIGVVEGRDVFIVDDMTETAGTLCAAAKIVRENGAGRIFAGVTHAVLGEMGCKRIMESEIDEVITTNSTPVSCPERVTQVSVAGLLGEAIRRISEGKSVSSLFDIDGPTV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining ribose phosphate diphosphokinase activity.2. Ideal protein should have a binding affinity for key enzymes in the 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1 pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "SPVLFDYLLSKNLPDIVVVSPDVGGVARARAFAKKLNDAPIAIIDKRRQTHNVAEVMNVVGDVAGKTAVLVDDMIDTAGTIHEGAKILRKEGARQVYACATHAVFSPPAIERLSGGLFEEVIVTNC",
    "response#2": "SPVLFDYLLSKNLPDIVVVSPDVGGVARARAFAKKLNDAPIAIIDKRRQTHNVAEVMNVVGDVAGKTAVLVDDMIDTAGTIHEGAKILRKEGARQVYACATHAVFSPPAIERLSGGLFEEVIVTNPK",
    "response#3": "SPVLFDYLLSKNLPDIVVVSPDVGGVARARAFAKKLNDAPIAIIDKRRQTHNVAEVMNVVGDVAGKTAVLVDDMIDTAGTIHEGAKILRKEGARQVYACATHAVFSPPAIERLSGGLFEEVIVTNR"
  },
  {
    "reference": "MSRLKGRRIVLGVTGSISAYKAAEVVRGLVREQADVRVVMTREAGAFVGAVTFSALTAHPVAVDQFPDTGMAGEEHVDLAVWADTVAIAPATANIIGKMANGLGDDLLSTVMLACEAPCCLAPAMNFRMWRNEAVQSNLDRLRSRGVHVIEPEFGLLANGEEGDGRLAAPEQIVEAVAILADPDHDLAGCNVLVSAGPTAEDIDPVRCVTNRSSGKMGYALARAAARRGASVTLVAGPTHLQDPYGVAVEHVRTAGEMREAVLAGREGQHAVIMAAAVADYRPGVQAPRKTKKTEERLTLEMTRTADILAEVAAARPPVLIGFAAETHDGLDYARRKRADKDLDLVVYNDLTAEGAGFETDTNIVTLIHRDGREESLPLQSKSDVADRVMDEVAALLRNREVVPA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should interact with enzymes and substrates within the CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis pathway to enhance or regulate its activity.2. The protein should have a fast turnover rate for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.3. The designed protein must be optimized for phosphopantothenate--cysteine ligase activity.4. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..5. The protein contains novel DNA/pantothenate metabolism flavoprotein C-terminal domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MNIVLGVSGGIAAYKAVALARLLVEAGHEVHVVPTADALRFVGQPTWEAISRNPVTTSVHDDVPEVRHVALGQNADLVVVAPATANTLARMTAGLADDLLGTTLLATRAPVVVAPAMHTEMWQHPATQDNVAILRARGVSFVGPESGRLTGNDSGPGRMSEPADIFAHVQPPERLGAVVADAFASPPLTGLRIMITAGPTREAIDPVRYISNHSSGKMGYALAAEAAAAGARVSLISGPVNLTVPEGVQVVQVVTAREMLDACLAGIDAQDVFVGVAAVADYRPAEVVSHKIKKTTDTLQLSLVRNPDIIGTLAARSPRPLMVGFAAETDNVEAYGEDKLTRKRLDLLFANEATTTFGSDGVAGTFFDADGTRSDLGFGSKAELAHHVWPLVRELVG",
    "response#2": "MNIVLGVSGGIAAYKAVALARLLVEAGHEVHVVPTADALRFVGQPTWEAISRNPVTTSVHDDVPEVRHVALGQNADLVVVAPATANTLARMTAGLADDLLGTTLLATRAPVVVAPAMHTEMWQHPATQDNVAILRARGVSFVGPESGRLTGNDSGPGRMSEPADIFAHVQEPRRIAEAVREVLERPALAGRRAVVTSGPTHEPIDPVRYIANRSSGRQGHAIAAALAGLGAEVTLVSGPVAIPDPANVRVRHVETAREMLDAVMTSLPADIAVMVAAVADWRVESLSDQKIKKAPGDAAPALILTENPDILKTIGHHAERPAVVVGFAAETQSVLANGRDKLERKGADLIVANDVSPRTGIMGGEHNQVTLLTPDGSETWPRMGKDTVARKLAERIADAIR",
    "response#3": "EVVLGVCGGIAAYKACELLRLFTESGHGVRVVPTASALKFVGEPTWAALSGRPVATEVWDDAHEVPHVRIGRAAELVVVAPATADILAKAAHGIADDLLTNTLLTATCPIVYAPAMHTEMWENPATRANVATLRERGAIVIEPAVGRLTGKDTGKGRLPDPVELAAAAREVLADGPLAGEKMVITAGPTREYLDPVRFISNRSSGKMGYALARAARDRGASVILISGPSYLKKPQGVGFVGVETSAQMMKSVKQSVQGDTTVLVMAAAVADFSPAVTSRTKIEKTGDLNIPFRQTEDIISSVLGAGKRPYIIGFAAETGNNIARAQKKMKVKNMDMVVFNDVTEAGAGFDVDTNKVVIIDNSGAVEVWPQLPKEEVAARLWDRVAALL"
  },
  {
    "reference": "MTDQAPVTYAGPPRVLSGVQPSGALHLGNYLGALVKFTRLQHEIDTFIFVADLHAITVWQDPAALAQQTREIAAAYIASGLDPDKATIFPQSAVREHAELSWIFNCVARLGWLDRMTQFKEKSGKHKERSSVGLYTYPVLQAADILIYKATHVPVGEDQKQHLELTRDIAQKFNHDFNAPGFFPLPDPLIQGPGARVMSLRDGSAKMSKSDPSDYSRINLTDTADDIAAKVKKARTDPEPLPETIEELATRAEADNLVGIFAALAGKTKAEVLADYAGKGFGTFKPALAELAVESLAPVGERMRGLLGDPAVLDAILAKGAEKAREAAAPTLAEVKKLVGFWGA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The ATP, L-tryptophan binding site should be stable and able to withstand changes in environmental conditions.2. The designed protein should have tryptophan-tRNA ligase activity within cytoplasm to regulate tryptophanyl-tRNA aminoacylation.3. The protein should be designed to have high stability and activity towards ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-   tryptophanyl-tRNA(Trp).4. The protein design should include a 'KMSKS' region motif to ensure proper function. The designed protein sequence is ",
    "response#1": "MNDPNGRRG",
    "response#2": "MKVLETERLILRPPQLSDAEGLYANWASDPRVTRFLGPRPVHSREESRQMILGVIGHYGPFGLFEIELKETKSFIGNAGLHLRDIDTHTAEIGYRLARAHWGKGYATEASRAVIRYGFTELGLNRIWSGAFADNVGSWRVMEKIGMRREAHTVRNAFFKGAPHDCVVYSILREEWLAREG",
    "response#3": "MKSAEIKKILPHRYPFLLVDRIENVDPLKKVTGIKNVTVNEPFFNGHFPNRPIMPGVLIVEALAQTAGVLLLKNAEDSGNKIYYFIGIDNARFRKPVVPGDQLVLEVEALKMTRSICKFKAKAYVNDKLACEAEVRFMMTDKA"
  },
  {
    "reference": "MKSEYTWDELLAKVADGLMVRSDSRAVQPGEVFVAIPGASTDATAYVPDAVARGAAYVVARSGQHMPAGTKAKLIVHENPRRALGELAAAYFKTREKGLTVVGITGTNGKTTISYMLEHLLAHAGHKVGVIGTVSYRWPGFTLEAPLTTPDCWQLHELVSNMAGSGVDTVVMEVSSHALDQDRIWGLDYDVAVLTNITQDHLDYHGNMDSYFRAKAKLFEEISKPGKVAILNADDSYGRILLQRYNPAIGYGLRYPNVGEGKGLSGEIRSMNSNGLVLKMKFGDDSWELSSPLIGRHNASNLLAVQGVGLALGLKPKKLKALEKFMGVPGRLERIENNRGLNIFVDYAHTPDALDNVLSGVRELDFKRLLCVFGCGGNRDKAKRPLMAEAVCRHSDVAVLTSDNPRHEVPLEIMNDVRPGLGGCAEVIEDPDRRQAIAKAIAEMGPRDVLIVAGKGHETYQDIGGERFPFNDAEVIRELAC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The meso-2,6-diaminoheptanedioate binding site should be stable and able to withstand changes in environmental conditions.2. We need a protein that can perform ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate with high efficiency.3. A protein that is capable of catalyzing reactions within the peptidoglycan biosynthesis pathway with high efficiency and specificity.4. The ideal protein would be one that plays a critical role in cell division, regulation of cell shape through ATP binding, tetrahydrofolylpolyglutamate synthase activity within cytoplasm.5. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.6. The designed protein have Mur ligase central domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MSKITSLECDSRKIKRGSLFVAVRGYNVDGHKFIPLVTLAGAAAIVCEELPERLESTVTYIKVPDSAVALGLLASQFYGDPSRKLKVIGITGTNGKTTSAFIIKNILQSNGHSVAMIGTLGYGLLDKLGGEHTTPDAVTLNKNLQEARDAGCKAVVMEVSSHALAQHRVAGIDFDAAVFTNLTQDHLDFHGDMESYYAAKARLFTEGRADKAVINIDDEYGQRMIEAIPAEVWTFGIDAAAHVRAREIRATPSGTVFELVTPWGESKVRTKLVGRFNVYNALAAIAVGLHEGVALSDIAAAVERIDAVPGRMERIAAPGQPLAVVDYAHTPDALDKALAGLRPLAAQRGGALWCVFGCGGDRDPVKRPMMAAVVEKQADRVVVTSDNPRSEKPAAIIGQILLGLARPEAAQVEADRARAIADTLAQAAPEDVVLLAGRGHEDYQILGDKVVHFDDREVAREAIERLTGGKL",
    "response#2": "MKLRDLLKGIEIVSVIGDTSIEISGITNDSREVCPGDLFIAYRGAQFDGHSFIQDALNAGAVAICAEQDQPSEFRIPWIKEYDIAQRQAEFAARFYGNPSKKMKLIGITGTNGKTTSAFIIKNILQSNKDIGLIGTIANGIGNKNLKSERTTPESLELQSLFSEMVEKGVEYVAMEVSSHALDQKRVDGCEFDIGVFTNLTQDHLDYHKTMENYYKAKAKLFSMTGRFETLRSDGGIFIVVDYAHTPDALENILDSINEIRTKNERLITVFGCGGDRDNAKRPEMGKIATRKSTLAIITSDNPRSENPEAIIKEIEAGVEPQNFSKYTSIPDRKEAIKMALKFAEPRDIILLAGKGKEAEFKVRKIGGRFETFKSDGGIFFIVDYAHTPDALENVLDSINDIRTKNERLITVFGCGGDRDHSKRPEMGNIASKKSTLAIITSDNPRTEDPNQIIKEIEAGVEPQNFSKYTSIPDRREAIKMAIKFSEPKDIVLVAGKGHEDYQIIGNRKYHFDDVELAREAVEKQ",
    "response#3": "MKLSRILKDSDLIAVEGNVDVALRTDSRQVQAGDAFIAWPGAATDGRQYVAQALAQGASACLMELPDPWPGKLGVPCILTSDARKAAARVAGRFYGDPSAALHVIGVTGTNGKTTTTWLLRSILETAGRTTGLLGTLEYFDGRTTEHPSLTTPDAEQLAHYIGRMARNQVTECVMEISSHALHQQRCAGLTLSAAAITNITRDHFDYHGDADKYRAAKLRIASLLACDKPILLGIDDAGCQAIRKTLIAERNVITFGFSADANMRVETLSCSPAGQSIRLSLDSSSIEIRTAMVGRHNALNLLVAAALAISAGLPGRMERIAAPGQPLAVVDYAHTPDALDKALAGLRPLAAQRGGALWCVFGCGGDRDPVKRPMMAAVVEKQADRVVVTSDNPRSEKPAAIIGQILLGLARPEAAQVEADRARAIADTLAQAAPEDVVLLAGRGHETYQIVGDTRIHFDDREVAREALR"
  },
  {
    "reference": "MITIKKGLDLPIAGVPTQVINDGNSITKVALLGEEYVGMRPTMHARVEDVVKKGQILFADKKNPGVIFTSPVSGKVVEINRGAKRVLQSVVIAVEGEEQVTFNSYAANQLASLDRDAIKQQLIDSGAWTALRTRPFSKVPAIDSETKAIFVTAMDTNPLAADAEVIINQQEDAFVAGLDLLSVLTGEKVYVCKKGGSLPRSSQGNVEEHVFDGPHPAGLAGTHMHYLYPVDLNNVAWSIGYQDVIAFGQLFLTGEIYSDRIVSLAGPAVNNPRLVRTITGASLLELTDSEVMPGEVRIISGSVLNGTQASGPHAYLGPYHQQVCVLREGREKEFLGWAVPGKNKFSVTRSFLSHVFSGQLFNMTTTTNGSDRAMVPIGSYERVMPLDMEPTMLLRDLCAGDVDSAARLGALELDEDDLGLCTYVCPGKYEYGPMLREILDTIEKEG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be designed to catalyze the following reaction a ubiquinone + H(+) + n Na(+)(in) + NADH = a ubiquinol + n   Na(+)(out) + NAD(+).2. The protein design must be able to perform oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor in order to contribute to sodium ion transport. The designed protein sequence is ",
    "response#1": "TGEQVRRNLQASGLWTALRTRPFSKVPAIDAVPSSIFVNAMDSNPLAADPAVIINEYAEDFTNGLTVLTRLHDKVNLVKSAGSNITAVGAVKVHEFGGKHPAGLVGTHIHFIDPVSLTKTVWHLNYQDVIAIGKLFTTGEG",
    "response#2": "TGEQVRRNLQASGLWTALRTRPFSKVPAIDAVPSSIFVNAMDSNPLAADPAVIINEYAEDFTNGLTVLTRLHDKVNLVKSAGSNITAVGAVKVHEFGGKHPAGLVGTHIHFIDPVSLTKTVWHLNYQDVIAIGKLFTTGEC",
    "response#3": "TGEQVRRNLQASGLWTALRTRPFSKVPAIDAVPSSIFVNAMDSNPLAADPAVIINEYAEDFTNGLTVLTRLHDKVNLVKSAGSNITAVGAVKVHEFGGKHPAGLVGTHIHFIDPVSLTKTVWHLNYQDVIAIGKLFTTGEV"
  },
  {
    "reference": "MNIIDRLEMEQMKKDIPAFKAGDTLRVHVKIVEGDKRRIQVFQGVCIKRHNKGLGSTFTVRKISDGMGVERVFPLHSPNIDKIEVMMVGRVRRAKLYYLRKLQGKAARIREKRSL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for stability and solubility when expressed in ribosome where it will be involved in translation. The designed protein sequence is ",
    "response#1": "MMKR",
    "response#2": "SRVRLESFNHELLTSACRKISDTIQNSDATKLSVVSLPTDKRIYCVLRSPHVDKDSREHFELRVHKRVIEIYYDPTVKCRIISKFRITVA",
    "response#3": "GRVRLESFNHELLTSACRKISDTIQNSDATKLSVVSLPTDKRIYCVLRSPHVDKDSREHFELRVHKRVIEIYYDPTVKCRIISKFRITVG"
  },
  {
    "reference": "MTLGSPRKGLLMLLMALVTQGDPVKPSRGPLVTCTCESPHCKGPTCRGAWCTVVLVREEGRHPQEHRGCGNLHRELCRGRPTEFVNHYCCDSHLCNHNVSLVLEATQPPSEQPGTDGQLALILGPVLALLALVALGVLGLWHVRRRQEKQRGLHSELGESSLILKASEQGDSMLGDLLDSDCTTGSGSGLPFLVQRTVARQVALVECVGKGRYGEVWRGLWHGESVAVKIFSSRDEQSWFRETEIYNTVLLRHDNILGFIASDMTSRNSSTQLWLITHYHEHGSLYDFLQRQTLEPHLALRLAVSAACGLAHLHVEIFGTQGKPAIAHRDFKSRNVLVKSNLQCCIADLGLAVMHSQGSDYLDIGNNPRVGTKRYMAPEVLDEQIRTDCFESYKWTDIWAFGLVLWEIARRTIVNGIVEDYRPPFYDVVPNDPSFEDMKKVVCVDQQTPTIPNRLAADPVLSGLAQMMRECWYPNPSARLTALRIKKTLQKISNSPEKPKVIQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases; May bind activin as well.2. A protein that contributes to negative regulation of gene expression by functioning in neuronal cell body, receptor complex with activin binding, SMAD binding, transforming growth factor beta receptor activity, transforming growth factor beta receptor activity, type I, ATP binding.3. The designed protein must have at least one Protein kinase domain.4. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.5. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "CCRRDHYVDFQELGWRDWILQPEGYQLNYCSGQCPPHLAGSPGIAASFHSAVFSLLKANNPWPAGSSCCVPTARRPLSLLYLDHNGNVVKTDVPDMVVEACGH",
    "response#2": "TVISHIGVGAFGKVSLVRKNDTRKVYAMKSLEKADVIMKQQAAHVKAERDILAEADSPWIVRLFFSFQDDACLYFIMEYVPGGDMMTLLIQKGIFEEDLARFYIAELACAIEYVHNVGFIHRDLKPDNILIDQHGHIKLTDFGLCTGLRWTHDRRYYGPENDHHRVDSFSLPPEVAAIDKSVKVLNVRQQTRRITAHSLVGTGNYMAPEVIAKTGHNQSCDWWSTGVILYEMVFGRVPFHDDTA",
    "response#3": "LCFSQVIREGGHAAVWAGQLQGELVAIKVFPRRAVAQFRAERALYELPGLQHNHVVRFIAAGQGGPGPLPSGPLLVLELHPKGSLCQYLSQHTSDWGSSLRMALSLAQGLAFLHEERWQDGQYKPGIAHRDLSSQNVLIREDGSCAIGDLGLALVLPGFAQPRAWAPPQPRGPAAIMEAGTQRYMAPELLDKSLDLQDWGTALRRADVYSLALLLWEILSRCPDLRPDGRPPPFQLAYEAELGSAPTTCELWALAVEERRRPDIPSSWCCFATDPGGLRELLEDCWDADPEARLTAECVQQRLVALVHPQL"
  },
  {
    "reference": "MLTGKRILLIVGGGIAAYKTLDLIRQLRRAGASITPVLTRAGAEFVTPLSVASLAGEKVYTDLWDLTDEAEMGHIQLSRVADLIVMAPATADLMAKMAGGHADDLASTLLLATDTPVLIAPAMNVRMWQHPATQRNLDLLRQDGIGTVGPDVGDMACGEHGPGRMSEPAAILAEITSRLTAGPLTGRHVLITSGPTHEPIDPVRYIANRSSGAQGTAIARAARRAGARVTFVTGPADAPLPEGCTIHRVETARQMHETVTQALPADVAIFAAAVADWRVSNAAASKMKKTAGGLPQLEFAENPDILACVSTRADRPGLVIGFAAETDDVVANAITKRRRKGCDWIVANDVSDGTGIMGGTENAVTLVTETGTEDWPRAGKDVVAERLIARIAETLR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A2. A protein should be able to manipulate the activity of enzymes in the Cofactor biosynthesis pathway, either by activating or inhibiting their function.3. The designed protein must have a Proton donor active site that is highly conserved among related enzymes.4. A protein with phosphopantothenate--cysteine ligase activity is needed to enhance pantothenate catabolic process in a specific context.5. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal domain. The designed protein sequence is ",
    "response#1": "MAGRHVLVTAGGTREHLDPVRYLGNISSGKQGYAFARTAVARGARVTLISANVSLPDPAGVEVVRVGSTAELRTAVLEHGDDADVVVMAAAPADFRPTKYAEQKIKKAVDGTAPVIELTTNPDIAAELGAQRRPGRILVAFAAETQDFVRNAQQKLTRKNADLIVVNEVGNGKAFGTDDNAVTVLLLR",
    "response#2": "MAGRHVLVTAGGTREHLDPVRYLGNISSGKQGYAFARTAVARGARVTLISANVSLPDPAGVEVVRVGSTAELRTAVLEHGDDADVVVMAAAPADFRPTKYAEQKIKKAVDGTAPVIELTTNPDIAAELGAQRRPGRILVAFAAETQDFVRNAQQKLTRKNADLIVVNEVGNGKAFGTDDNAVTVFSYV",
    "response#3": "LEGKKFLISGGRTEEDIDPVRTITNRSSGRMALELLYAAVCRDGMARGVFGEVSVSVPEEMNSVRVRTSSEMLKECKRWLGWSDCLIMAAAVGDYKPKKKSQRKIHTDTLTLQLVKNVDVLAELSKSKRGQVMVGFSLDRKDHVKRGKEKLAKKGLDLIVLNEPSVLGSGLLDNENIKKG"
  },
  {
    "reference": "MRKDGARLALPLFDPRHDPGPDFAALVSRDAARTVPTSGDGSLGAQVPHGTTIAALRFAGGVVIAGDRRATEGNFIANRTIEKVFPADRFSGVGIAGAAGPAVEMVRIFQVQLEHYEKVEGKALSLEGKANQLAQMIRQNLPLAMQGLVVMPLFAGWDAERSEGRIFTFDVAGGRYEEVAYYAIGSGGRDARATLKLGWRPGLDEAGAVHLAVQALYEAAQEDAATGGPDALRGIFPVVAVIDREGYRRIDDARLEEIAVELDQAVRERGARR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that can modulate the overall flux of metabolites through the Protein degradation, proteasomal Pup-dependent pathway pathway, enhancing or suppressing its activity as needed.2. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile to enable effective catalysis of Cleavage of peptide bonds with very broad specificity..3. The designed protein should be involved in proteasomal protein catabolic process, modification-dependent protein catabolic process by localizing to proteasome core complex, beta-subunit complex, cytoplasm and exhibiting threonine-type endopeptidase activity.4. For general function, the protein need meet that Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The designed protein sequence is ",
    "response#1": "PPKGVLLYGPPGCGKTLIAKAVAASLAGMVDPHGTEGATSYFLNVKGPELLNKYVGETERHIRLIFARAREKASQGMPVVVFFDEMESLFRTRGTGLSSDVETTIVPQLLAEIDGVERLDNVIVIGASNREDMIDPAILRPGRLDVKIKIERPGHEDLLEILQKVCTKTNMKLPEEVAEELLERTESDLRSAINNLQAVALGKSRITVQDVQASQSAYEKKTVFQLIGEIFQAKNPRVSLELAKKQGVTPEFLLQDVTDNVHALVQLDSLSEAFKAVDLFVRADFRTGGLGRNQGFSHYLLLHLGVFRALSQKSEGRGQFEFPDYHYKLRTLTQGTRQTIRAVQTLALELARDVCWGREEMVPDLVRVLGLLLAPGAQALQPDPVLSSKAAQKEKQPVSKRQKLHES",
    "response#2": "MVLLYGPPGCGKTLIAKAVANSLAKKVAEVTGKPEGKSFFLNIKGPELLNKYVGETERHIRLVFQRAREKASGGWPVIVFFDEMDSIFRTRGSGVSSDVENTIVPQLLSEIDGVEGLENVIVIGASNREDMIDPAILRPGRLDVKIKIGFEGGQMPLQRRIPRGFKDINAKEYVTVKEVLDGDRVLVVGRADEERVVRLAGSVRDLPLRVGDSLTVDVRSGFVFEVVD",
    "response#3": "PPKGVLLYGPPGCGKTLIAKAVANSLAKKVAEVTGKPEGKSFFLNIKGPELLNKYVGETERHIRLVFQRAREKASGGWPVIVFFDEMDSIFRTRGSGVSSDVENTIVPQLLSEIDGVEGLENVIVIGASNREDMIDPAILRPGRLDVKIKIERPGHEDLLGILNEVSEGLGFPVADDVLAAITDRLDHNVRALEGALTRLTTMSALNRKAIDIPTAEAALRDLIEAGPETVIQVVMFKELLDGDRALVIAHADEERVVMLADSLADTPLRVGDSLLIEPRSGFAYERIRAGVMVCALREVLDDGGPEGSVARALVVGHADEERVVWLAQPLLDSPLKAGDSLLVDSKAGFAYERVIR"
  },
  {
    "reference": "MAEKLSEQQIAEFKEAFSLFDKDSDGKITTKELGTVMRSLGQNPSESELTDMINEVDVNSDGSIDFPEFLTMMARKMKDTDSEAEIAEAFKVFDRNGDGKISAAELRHLLTSIGEKLSDADVDQMIKEADTNNDGEIDIQEFTSLLAAK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a binding site that is specific to the target Ca(2+) ligand.2. The designed protein must have at least one EF-hand domain.3. The designed protein must be able to perform calcium ion binding under specific conditions. The designed protein sequence is ",
    "response#1": "GEEVKKAFFVIDQDQSGFIEEDELKLFLQTFGAGARELTAAETKAFLAAGDEDGDGMIGVDEFVTLS",
    "response#2": "EAAQLLKHLDRDKSGKISSQELMEFLHTVNCPFKKEQVEKFIKQHDKDGDGQLNTDELLDVM",
    "response#3": "MGKEIVKEAFRLFDENQDGFIDENELKHVLSLLGYK"
  },
  {
    "reference": "MSFNTFGHMFRVTTFGESHGVAIGCVVDGCPPMIPLTEADIQGDLDRRRPGQSRFTTQRQEPDQVKILSGVMAHPETGVQVTTGTPIGLLIENTDQRSKDYSEIKDKFRPGHADFTYEAKYGLRDYRGGGRSSARETATRVAAGAIARKVLPDVKVRGALVQMGPHKIDREKWDWDEVARNPFFCPDKDKAAFFETYLDGIRKSGSSIGAVLEIVAEGVPAGLGAPIYAKLDSDLAGAMMTINAVKGVEIGAGFGAAELTGEENADEMRTGNDGTRFLSNHAGGVLGGISTGQPVVVRFAVKPTSSILQPRLTVDRQGADTEIMTKGRHDPCVGIRAVPVGEAMMACVLADHFIRDRGQVGR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must be engineered to have a cooperative binding mechanism with FMNH2 to ensure proper regulation.2. A protein that contributes to amino acid biosynthetic process, chorismate biosynthetic process, aromatic amino acid family biosynthetic process by functioning in cytosol with FMN binding.3. The protein should have a high affinity for FMN, NADP(+).4. We are looking for a protein with broad substrate specificity for 5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate +   phosphate.5. For general function, the protein need meet that Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis; This reaction introduces a second double bond into the aromatic ring system.6. The protein design should take into account the physiological conditions of the Metabolic intermediate biosynthesis, chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7 pathway. The designed protein sequence is ",
    "response#1": "MKLLKDILYKVAISAVEGNTDIXVXGVXDXRXMXNXFFAIKGATFNGNDFIDKTIENGAIAVVSEREVKIAEGXSXIIXVKDTKESLSYIASNYYDNPSKKXKLIAGRSSARLTAPVVGAGATVRSDPTALPAAGMVMEAGVEIGKDTIIHPNVTIREKXIIGNRVIIESNAVIGAXGFEFTPXGXGXKXIIQHSGKVIIQDNVEIGANTTIDRGALDXTIIGHGTKIDNQCQIAXSVGIGSFTIIRHDPCVGIRATPIAEAVGIGGVLEPLQKGKXVAXGXVVIKXVKDYXKXTGVYPSXGPVVXGXKEFYXIXIKXNDXKSXKXXIXILDKIXKK",
    "response#2": "MMGDKQTIGESHGEAMGVTVSGVPPLLTVDKINAELARRRLGYGRGARMKFERDEVEITSGIRFGQTLGSPITLRVVNKDWLNAERWMQIEGGEASPADFKTPARPGHADLAGMIKYGFREPGRSSARETAVRVAAGAVAKMLLHQFDVQVRAHVVAIGSLSAEPFAFDEVMASIERDENALAAAHDEAIIELIDRARKEGDSVGGIVETVLRGIPLGLGSYVQWDRKLDARLASALMSINAVKGVEIGDGFAAASMRGSENNDEMLPADGFIGKSNNAGGILGGISTGQDIVVRIAVKPTSSIRKPQRSINRAGEDVDVITKGRHDPCVGIRAVPVAEAMVALVLADMVLLQKAR",
    "response#3": "MKLSGMGESHGEALIAVIEGLPSGIRITVENINAELARRRLGFGRGARMKFEQDKVEIVSGVRHGKTTGSPIAVEIINKDWENWEKVMDPDDPRYGTKGRPEPGHADFPGLIKAKKPVRTGGERSSARETTMRVAVGAIAKKLLRMLGVEIFSHVLAIGDIETDENDYPNIDDLMANIEKNDDSSVGTEVKEAMIGIAKKGDTCGGEIYDRIKNCISEGDTLGGIIECRVVGLPAGLGDPIYAKLDADLAKAIMSIGAVKGVEIGEGFDAAKLRGSEMNDEYCFKNGEMRRISNHNGGIIGGISTGEDIVVRFAVKPTPSITKEQKTVNVNGEATTMEIKGRHDPCVGIRAAPIAEAMVALVLVDSLLLQKGIK"
  },
  {
    "reference": "MENRWQVMIVWQVDRMRIRTWKSLVKYHMYVSKKADKWFYRHHYESRHPRVSSEVHIPLGDARLVVTTYWGLHTGERDWHLGQGVSIEWRERKYSTQVDPDLADQLIHLYYFDCFSESAIRQAILGHIVSPSCEYQAGHKKVGSLQYLALTALIKPKNIKPPLPSIRKLTEDRWNKPQKTKGHRGSHTMNGH",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should contain at least one BC-box-like motif, HCCH motif motif to achieve its intended function.2. For general function, the protein need meet that May interact directly with APOBEC3G mRNA in order to inhibit its translation; Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism; Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions; Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G; Recruits an active E3 ubiquitin ligase complex composed of elongin BC, CUL5, and RBX2 to induce polyubiquitination of APOBEC3G and APOBEC3F; Finally, Vif also contributes to the G2 cell cycle arrest observed in HIV infected cells.; In turn, they are directed to the 26S proteasome for degradation; Seems to play a role in viral morphology by affecting the stability of the viral nucleoprotein core3. The designed protein should have RNA binding within plasma membrane, cytoplasm, virion component, host cell plasma membrane to regulate viral life cycle. The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPAVRERMRRARPAAEGVGAASQDLDKYGALTSSNTATNNAACAWLEAQEEEEVGFPVRPQVPLRPMTYKGAFDLGFFLKEKGGLDGLIYSKKRQEILDLWVYNTQGFFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPREVEEDTKGENNCLLHPMSQHGMEDEDREVLMWKFDSSLARKHLARELHPEFYKDC",
    "response#2": "MGSKWSKSSIVGWPAIRERIRRTDPAAEGVGAASQDLEKHGALTSSNTPATNADCAWLEAQEDEEVGFPVRPQVPLRPMTYKAAFDLSFFLKEKGGLDGLIYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPKEVEEANEGENNCLLHPMSQHGIEDADREVLMWKFDSSLARRHMARELHPEYYKDC",
    "response#3": "MGVVCRLLVLLTVLWVCEGFPQPHSKRQAAAAPKNGAPAPATAKKPAAQPAQPAKPAKPATPAKPAKPAKPAAPATTAAPAAPAAPAAPAAPAHKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAAPATPAAPAAPAAPAAPAAPAAPSHKPAKPAKPAKAAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKAAKPAAKPKKK"
  },
  {
    "reference": "MNAIVAEKMETQALIDYLASHEQKSLLRFLTCGSVDDGKSTLIGRLLYDTKLIFEDQLASIEKDSKKHGTVGDDIDLALLVDGLQAEREQGITIDVAYRFFTTDKRKFIVADTPGHEQYTRNMATGASNSDLAVILIDARKGILTQTRRHSFIVSLLGIKHVVLAVNKIDLMDYSQAVFDKIVADYKAFAADFGFETLQAIPMSARYGDNVLSRSEKMAWYDGPTLVEHLETVQIDQDLSDKPFRLQVQWVNRPDLNFRGFSGTIASGTIRPGDEIIVANSGKSSTLKSIVTYDGELPVAGAGQAVTLTLADEIDISRGDVLTGKEARPELSDQFQAHLIWMSDQELIPGRTLLIKIGAKTVSASVTEIKHKTDVNTFANLAAKTLKLNEVAVVNVALQEPVAFDTYADNHAMGAFIVIDRVTNLTLGAGMIDHGLRRATNVHWQALDVNKATRAAAKNQKPAVLWFTGLSGAGKSTIANLVEKKLLSLGHHTYLLDGDNVRHGLNKDLGFTDADRVENIRRVAEVSKLMVDAGLITLVSFISPFRAERQMAREMLADGEFIEIYVSTPLAVAEQRDVKGLYAKARAGEIKHFTGIDSPYEAPESQQLTLNTATQAPEQLAEKVVTYMIEHGLLGDGSDYMI",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: APS synthesis involves the formation of a high-energy phosphoric- sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD..2. The designed protein should be optimized for GTP binding, ATP binding, GTPase activity activity in order to improve hydrogen sulfide biosynthetic process, phosphorylation, sulfate assimilation.3. The protein design should consider the endogenous proteins and enzymes present in the hydrogen sulfide biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "DMLRVLTCGSVDDGKSTLIGRLLFDSKMIFEDQMAAIEKDSKRFNTTDDSFDLALLVDGLQSEREQGITIDVAYRYFTTEQRKFIIADTPGHEQYTRNMATGASTCDLAIILIDARHGVQVQTRRHSFICSQLGIKHVIIAINKMDAIDYDQATYQKIKKEYREFAEDLSFSDVRFVPISALKGDNVVNESPNMTWYPGSRFPVQYVIRPHQDFRGFAGRMISGTFRPGEEIVALPSGRTSHIKSIVTYDGDVQEAFPPQSVTLTLTDEVDISRGDMIVRPEMSDQFEARMVWMHDEPGHVGRTYDLKLANQWASASITSLKYRVEVNTQAHESCKQLELNDIAAANLALSKPVVFAAYAENPTLGGFILVDKFTHATVAAGMEVSDQFDCQLVWLDSEEGYIGRTYWLILGSERVNATLSEIKFKYNVNTLEELPARSFELNDIGRVTLSCDKAIPFETYEACREMGSLVLVDRFSNSTVGAGMITRSLK",
    "response#2": "KDMLRFLTCGSVDDGKSTLIGRLLHDSKMIYEDQLAAITKDSKKVGTTGEKVDLALLVDGLQSEREQGITIDVAYRYFSTDKRKFIIADTPGHEQYTRNMVTGASTCDLAIILVDARGGVKVQTKRHSFLVSLLGIKHVIVAINKMDLMDYSEEVYKQIQEDYLKFAEQLDIPDIQFVPISALEGDNVVGKSEKTPWFDGTPLMEMLENIEIGEDDNFRFPVQYVNRPNLDFRGFAGTVVSGQVAPGDEVTALPSGKKSKVKQVVTFEGDQERAYVPQAVTLTLEDEIDISRGDMIVHPNREVTDQFEAVLIWMNEDAGLNGRSYEIKLATQSASAAITNIKYRIDTNTLAHQASKHLSLNDISVCTLATSKPLVFDTYQNSKTLGSFILVDRITHATVAAGMEVSDQFDCQLVWLDSEEGYIGRTYWLILGSERVNATLSEIKFKYNVNTLEELPARSFELNDIGRVTLSCDKAIPFETYEACREMGSLVLVDRFSNSTVGAGMIR",
    "response#3": "RFLTCGSVDDGKSTLIGRLLYDTKLIFDDQLATLEKDSKKHGTTGEEIDFALLVDGLEAEREQGITIDVAYRFFTTDKRSFIVADTPGHEQYTRNMATGASNSDAAVILIDARKGVLTQTKRHSYICALLGIRHVVVAVNKIDLMGHSSQVFERIVEDYLAFAAALGFASITPIPISARYGDNVTVRSANMAWYDGPTLLDWLESVDVRFTTAGSVDDGKSTLIGRMLYDTKSLFEDQIAQVEQTSHDRGDEYVDLALFTDGLRAEREQKITIDVAYRYFATPRRKFIIADTPGHIQYTRNMVTGASTAELAIVLIDARKGVQTQSKRHGFIATLLRIPHIVVAINKMDLVHYDEAVYDRIVDEYSSFISKLNAQDVTFIPVSALVGDNVVDKSAYMPWYEGPTLLHHLEHVNQ"
  },
  {
    "reference": "MQRVPSGQPHPLYSADATRALEAALASTLAPHALMQRAGLATARLALAIAPHARRYWIACGPGNNGGDGLEAAMHLQQWGKTPLVTWLGTADTAPTDARASHARAVAAGVRFVDAPPADCDICIDALLGTGANRAPEGRLADWITHINSLAVPVLAVDLPSGLNADTGATPGPCVRASHTLCLLTLKPGLYTAQGRDQTGAVWYESLGADLGSTAPQARLGGAPATARRRHATHKGSYGDVAVIGGARGMSGAALLAASAALHHGAGRVYVGLLDDDDMTVDPTHPELMFRVPTALDVAAGTVVAGCGGGQAIAAHLPRLLSTAPRLVLDADALNSIANDSQFATLLAARGARQRQTVLTPHPLEAARLLACTTAAVQADRLAAAQKLAQQFGCTVVLKGSGTVMAAPGRLPVINPTGNARLATAGTGDVLAGLIGARLAQGEHAFEAACAAVWLHGLAADAWPPDQPLTADALARRG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should contain one or more YjeF N-terminal domains that are essential for its function.2. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..3. The designed protein should be optimized for NADPHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADHX epimerase activity, ATP binding activity in order to improve nicotinamide nucleotide metabolic process.4. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.5. The designed protein should be able to compete with endogenous proteins or inhibitors for K(+), AMP, (6S)-NADPHX binding. The designed protein sequence is ",
    "response#1": "MFTPLYTADEMRAAEEGHDVDAMMERAGRAVADAVLRRYPDARRIAGVCGRGANGGDGRIALRLLAEAGRETSEELDGADVVIDALFGTGFQGVPRDDAASSIERINSSRAPVVAVDLPSGVDASTGEVAGAVVDADVTVTMHASKVGLAIAPGRFHAGDVDVADIGIGPALWSERFPWPTGASHKHVRGRLAVVSGQQTQTGAARLAARAGLRIGAGVVTVLSPPSAVAVNAAHLEAIMLRAFDNEAQLVEGADNADAVVIGPAAGVTPETRGNLFALAHTGAAMVVDADALTVFRDRPGELFATLDRDDVLTPHPGEFERLFPGLLDGAPHRLAAAREAAVKAGAIVILKGADTVIASPDGRAAVNVEDAPWLATAGSGDVLAGFVAGLIAQGMESFEAACAGAWIHAECARRHGPGLIAEDLPQLSSAVLRDLYPPSPERLKWGHKGSFGKLLVVAGARYYYGAPYYSSYSFLKAGGGYSRLAAPKSVIPVLASRCSEVVYHPMDETTEGSLSMGNYESILRIIDEHGVDIVVLGPGTSLNEETQELIRKLAGAINKPLLIDGDGLTAISKDPSIIKKRKAGTILTPHPLEFTRLTGVDLSDLREDPVGYIRRASQDLNSYIVLKGAHSLIANPDGRVYVNMTGNPGMAKAGMGDVLNGVITAMYGVGFRDLGEATRMGVLVHGLAGDLAAEEVGEDGVTPEAVMEYLPRAVGPALWSERFPWPTGASHKHVRGRLAVVSGQQTQTGAARLAARAGLRIGAGVVTVLSPPSAVAVNAAHLEAIMLRAFDNEAQLVEGADNADAVVIGPAAGVTPETRGNLFALAHTGAAMVVDADALTVFRDRPGELFATLDRDDVLTPHPGEFERLFPGLLDGAPHRLAAAREAAVKAGAIVILKGADTVIASPDGRAAVNVEDAPWLATAGSGDVLAGFVAGLIAQGMESFEAACAGAWIHAECARRHGPGLIAEDLPQLSSAVLRDLY",
    "response#2": "MFTPLYTADEMRAAEEGHDVDAMMERAGRAVADAVLRRYPDARRIAGVCGRGANGGDGRIALRLLAEAGRETSEELDGADVVIDALFGTGFQGVPRDDAASSIERINSSRAPVVAVDLPSGVDASTGEVAGAVVDADVTVTMHASKVGLAIAPGRFHAGDVDVADIGIEPTEVGARWPVPGPASDKYTQGVTGVAAGSAEFPGAAVLASGGAVLATSGMVRFAGSAAEAVGNRWPEVIVTGSVTDAGRVQAWVVGPGIGTGSAGRDVLARVLADGVPVCADADALTLLAQHPELADERDPDVALVLTPHDREFARLTGEEPGADRATAARDAARRFGAVMLLKGNTTIVAAPDGRVLANPARSAWPATAGSGDVLAGIIGALLAAGLEPATAAAMAVHAHESAADLAANGAPAPASMLVTALTEAIRRTDDDVADLLPRPEAESDKYRRGVLGIVAGSDRFTGAAVLAVGGAIRAGAGLVRFASAETAVRQVRNRWPEAITTVLDGPSVAEVGRVQAWVLGPGMGTGEEAVALARAVLATDVPVLVDADGLTILANDRSLLRRSAPTVLTPHAGELSRLLDVPRERIEAARMEHVRRAVAELGAIVLLKGSTTLIAEQDRPVRVNTTGTPWLATGGTGDVLSGIAGTLLSGGLSGYDAVSCAAHLHGLAAQAASTGISGGHGPIAAMDVATMLPQVIRSG",
    "response#3": "MRVIEENAVFLGVSGLLMMENAGRSIADLVLQFTRKIKRKAKVVVVAGLGNNGGDGLVAARHLASQGFPVTVIMADDPRLMKREEVIRNFDIIKQMRLSVKLLVYRKNMDAVKEAIENADIIIDALLGIGLQGRVREPYASLIQLINNSKGIKVSVDVPSGLDADSGNPLGPCVKADYTITFHKMKQGLVNNEYAGRIHVADIGIPLEAELTTACIPQSGLVALNSYMPELMAIIRKDGKLPDIEWDKFSTIAIGPGLGKDTEALKLLKAILKNYKKPIVVDADALNLLSENKTLLKKLPAGSVLTPHMKEFDRLFGTHTSWWQRIQTAKDKAAELNIIIVLKNDYTITATPEGKLYFNSTGNPAMATGGMGDVLTGIISALLAQKYSPQDACILGIYIHGKAGDELALPNRMNVTLPGKIIQELKDKYTQGVVGVLAGSAQYPGAAILCSGAAVAATSGMVRYVGSAAADVVTHYPEVVAATGMKEAGRVQSWVVGPGFGTDTSSRDVLSQVLTSDLPVVVDADALTILSSSPELVRGRTAPTLLTPHAGEFERLTGAAPGSDRVSAVRELASTWGVSVLLKGRATVIAGPDGAVVVSDAGGSAASTAGSGDVLAGILGALLASGLDTVTAAACAARVHALAAATAAHGRP"
  },
  {
    "reference": "MSKKRIYEYAKEVSLKSKDIIDALKKMNIEASSHMQVIEAKEIAALDKIFKKSDTKSEVKSEQKAEAKKEEPNKEQPKKEEPKKQSDQKRPQNNNQKRNPNHRPGSHNKPGTGGPSKNKKGKGKGKQKPEPKQEVPVVKETPSKITYEEGITVGELAEKLGKDASEIVKNLFMVGIMANINQSLNQEAIELICDEYGVEAELEVVVDATDLETYFEDVDANEEDIMERPPVVTIMGHVDHGKTTLLDSIRNTRVTAGEAGGITQHIGAYQIEYNDKPITFLDTPGHAAFTTMRARGAQVTDITILVVAADDGVMPQTVEAINHAKAAEVPIIVAVNKIDKPTANPDRVMQELGEHGLYPEDWGGDTIFVQLSAIKGDGIDDLLEMIQLVTEVEELKANPKRTAIGTVIEAELDKSRGPAASLLVQDGTLEIGDSIVVGNTFGRVRAMVNDLGKRIKTAGPSLPVEITGLQDVPLAGDRFVVFKDEKKARRIGEARQQQNILAQRQESQKVSLDNLFEQMKQGEMKDLNVIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLASASNGIIIGFNVRPDVNAKRAAEAEGVDMRLHRIIYKVIEEIESAMKGMLDPEFEEKVIGQAEVRQTINISKVGTVAGSYVTDGKITRDSQVRIIRDGIVVYEGQVDALKRFKDDVREVAQGYECGITIENFNDIKEGDIFEAFVMEEIKRA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: One of the essential components for the initiation of protein synthesis; Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. The designed protein sequence is ",
    "response#1": "MEGASRLSVMCEDGYTRNARIPF",
    "response#2": "GRVILPNKKKGEMFGIVEKMEGASRLSVMCEDGYTRNARIPGRMRKRMWIREKDLVIVKPWEFQPEKADVVYRYR",
    "response#3": "MEGASRLSVMCEDGYTRNARIPF"
  },
  {
    "reference": "MSITPNAAISKSNIAKSTHAKSVVILAAGKGTRMYSDLPKVLHSLAGKPMVQHVIDTAMALGTQNVHLVYGHGGDLMKQTLSGQKNTNQNLNWVLQAEQLGTGHAMQQAAPHFADDEDILILYGDVPLIAQDTLERLVEAKPEGGIGLLTAILDNPTGYGRIIRENGEVTGIIEQKDATDEQLKINEINTGILVANGGDLKHWLSKLENNNAQGEYYLTDVIALAHQDGRQIKAVHPSRLSEMEGVNNRLQLSALERIYQSEQADKLLLAGVMLLDPARFDLRGTLEHGRDIIIDTNVIIEGHVVLGSNVQIGSGCILKNCVIGDDVVISPYTVIEDSELAAECTVGPFARLRPGSKLAEKAHVGNFVEMKKTSLGKGSKAGHLTYLGDSEIGDNVNIGAGTITCNYDGANKFKTIIGDDVFVGSDSQLVAPVSVAKGATIGAGTTVTKNVAENELVISRVKQTHIKNWQRPVKKK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.2. The protein requires Mg(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "TESYKSLNEALYHGGIPNDCRVNLIFVDSETIAPDTCSQALMEADGILVPGGFGSRGIEGKICTARYARENKVPYFGICLGMQVAVVEFARHVAGLEKANSSEFDRATPYPVIYLMTEWLDDKTGRIETRDESSDKGATMRLGAYPCRLRADTMASRAYGQENISERHRHRYEFNNAFRDQLEEKGLVVSGTSPGGELVEIVELPDHPWFLGCQFHPEFKSRPMAPHPLFREFIK",
    "response#2": "LGNDLIDIRRIERMLVRYGDRFVQRIFTDIEQNKSESLQKKSSSYAKRFAAKEACAKALGTGIACGVNWKDMGVINLPSGKPIMKLTNRAQMQLQKLG",
    "response#3": "LTWNIDGLDESNVAERARAVCSCLALYTPDVVFLQEVIPPYCEYLKKRAVSYKIITGNEDEYFTAMMLKKSRVKLISQEIVPYPSTLMMRNLLVANVNISGNSICLMTSHLESTKDHSKERLKQLDTVLKKMMDAPPSATVIFGGDTNLRDQEVAKIGGLPNTILDVWEFLGKPEHCRYTWDTKLNNNLRACYTSRLRFDRILYRASMEGSQVIPQFLNLVGTEKLDCGRFPSDE"
  },
  {
    "reference": "MEPVDPNLEPWEHPGSQPKTPCTKCFCKRCSYHCLVCFQTKGLGISYGRKKRRQRRSAPPSSEGHQDPIPKQPLPQTRGNPTGSEESKKKVESKTTADPCD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.2. For general function, the protein need meet that This binding increases Tat's affinity for a hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA) and allows Tat/P-TEFb complex to bind cooperatively to TAR RNA; Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation; Through its interaction with host TBP, Tat may also modulate transcription initiation; The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive; To ensure maximal activation of the LTR, Tat mediates nuclear translocation of NF-kappa-B by interacting with host RELA; Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication3. A protein that can perform cyclin binding, trans-activation response element binding, metal ion binding, actinin binding in host cell nucleolus, extracellular region, host cell cytoplasm to support suppression by virus of host type I interferon-mediated signaling pathway, positive regulation of viral transcription, negative regulation of peptidyl-threonine phosphorylation, positive regulation of transcription elongation by RNA polymerase II. The designed protein sequence is ",
    "response#1": "MEPVDPSLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFITKGLGISYGRKKRRQRRRPPQDSQTHQASLSKQPTSQPRGDPTGPKEPKKKVERETETDRRD",
    "response#2": "MEPVDPRLEPWKHPGSQPKTACTNCYCKKCCFFCQVCFIKKGLGISYGRKKRRQRRRAPQDSETHQVSLSKQPASQPRGDPTGPKESKKKVERETETDPDP",
    "response#3": "MGNTKSKQSGRGWKAVRERMTRTEPAAEGVGAASRDLEKYGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQQRQDILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPEKIEEANEGENNSLLHPMSLHGMEDPEKEVLEWRFDSRLAFHHMARELHPEYYKDC"
  },
  {
    "reference": "MYTPEAIGTPLTPSSLKVLLLGASELGRELAIALQRLGIEVHAADRYAGAPAHLVANKAHVLDIHDEDAIRQLVREVGPHFVIPDSETIPAEVLQEIETNEVASVVPTALANRLTMNREGIRTFAHDKLGLPNSTFRFASTADELARAAEEIGYPCVVKPVMSSSGAGQSYVGGVDELEAAWNAALKHSRTNAQRVMVEQYIPFDYEVTILAVRSIDPATGREATWFCEPIGHRQDRGDYVESWQPAHMSEDALDTARSIAARIATALGGRGVYGVELFVKGDDVYFSEVSPRLHDTGMVTLGTQRFSEFDLHARAILGLPIDTTLISPGASAVIRCEDKHDGDIEYAGVNKAMAVEETNIYLFGKPRALARRRMGVAVATAENIDQARQRAEEAAGYIEVRPTVFAEGGQ",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a fast turnover rate for ATP + formate + N(1)-(5-phospho-beta-D-ribosyl)glycinamide =   ADP + H(+) + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide +   phosphate.2. The protein contains novel ATP-grasp domains that confer a unique function or activity.3. For general function, the protein need meet that Involved in the de novo purine biosynthesis; Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.; Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR)4. The N(1)-(5-phospho-beta-D-ribosyl)glycinamide, Mg(2+), ATP binding site should be stable and able to withstand changes in environmental conditions.5. A protein with ligase activity, phosphoribosylglycinamide formyltransferase 2 activity, phosphoribosylglycinamide formyltransferase activity, magnesium ion binding, ATP binding functions is required to enable 'de novo' IMP biosynthetic process. The designed protein sequence is ",
    "response#1": "MRILIIGSGAREHAIAETLLRREVDAAEAGAEAEARERPTLYCLGTHRNPGIEALTAAPVTVGDITDPALAVETARAVGAELACIGPEAPLAGGVTDALEAAGIPVVGPRQGPARIEAAEELDLPTSPYHFADTFEDYSKAVADLGYPCVVKPVMSSSGKGQSLLRSADDLQKAWDYAQEGGRAGKGRVIIEGFIDFEYEITLLTVRHVGGTTFLEPVGHRQEKGDYQESWQPQAMSPKALAESQRVAQAVTDALGGRGLFGVELFVKGDQVWFSEVSPRPHDTGLVTLISQELSQGRVDLASKDLKIKTAKFDYIFEFDDLEKKADEIGFPLLLKPLMSSSGKGQSLVETKNDLQNAWKQAQANSRGKVKGVIIEEFINFDFEFTLLTVRKENGENIFCLPIGHLQSNGDYQCSWQPLEIKESLIIEAKRMTSRILNNLNGAGLYGVEFFIKGSEVIFSELSPRPHDTGMVTLVSQNINEFELHLRAFGGLESDPTPKALPILVSSSDGVGTKLKVALTCNSHESIGQDLVAMCVNDILVHGAEPLFFLDYYATGRLEGGAVVQVVKGIVDGCHQSGCALIGGETAEMPGLYHNNDYDVAGFAVGAAVRVLEPARVASRSASCALLAHGEGVPYFNNVAAALQVPDTAVRLFGKPSVHGHRRVGVTLARAETIDEARAIARDAAEAGVDLV",
    "response#2": "MRILIIGSGAREHAIAETLLRREVDAAEAGAEAEARERPTLYCLGTHRNPGIEALTAAPVTVGDITDPALAVETARAVGAELACIGPEAPLAGGVTDALEAAGIPVVGPRQGPARIEHEELGLPTTPYRFAGSLEELRAGASEVGYPCVVKPVMSSSGHGQSVVRSADAIDAAWTEAQEGRRAADEGDVSRVIVEALAPLERELTVLTVSSSAGIVTCAPIGQRQESGDYRESWQPATEPDGEAERARDIARTAVEGLVAKAQAAGETGWGVFGVELFVLTDGSILFNEVSPRPHDTGMVTMASQRLSEHEELGLPTTPYRFAGSLEELRAGASEVGYPCVVKPVMSSSGHGQSVVRSADAIDAAWTEAQEGRRAADEGDVSRVIVEALAPLERELTVLTVSSSAGIVTCAPIGQRQESGDYRESWQPATEPDGEAERARDIARTAVEGLVAKAQAAGETGWGVFGVELFVLTDGSILFNEVSPRPHDTGMVTMASQRLSERDLKIKTANYDYIYKFEDLEKKADEIGFPLLLKPLMSSSGKGQSLVGSKNDLNQAWSQAQANSRGQVKGVIIEEFINFDFEFTLLTVRKNNGENIFCLPIGHLQSNGDYQCSWHPLEINQSLISEAKKMTTKILNNLNGSGLYGVEFFVKENEVIFSELSPRPHDTGMVTLVSQNINEGVAEVRFCVGVVMASRGYPLTSSKGDIISGLPSVTKGMVFHMGTKLNNYGEVVTNGGRVLIVVTTHQDLVLAAARATMACGRIIFNGAQFRTDIAHRGG",
    "response#3": "MRILIIGSGAREHAIAETLLRREVDAAEAGAEAEARERPTLYCLGTHRNPGIEALTAAPVTVGDITDPALAVETARAVGAELACIGPEAPLAGGVTDALEAAGIPVVGPRQGPARIEAAETLGVPTSPYKFCDSLAELQAAIDAGIGYPCIVKPVMSSSGKGQSKIDGPADVQKAWDYAMAGGRVSHGRVIVEGFIDFDYEITLLTVRAKDAAGAVQTRFCEPIGHVQVSGDYVESWQPHPMAPAALQKAQQIAEAVTSDLGGQGLFGVELFVKGDEVWFSEVSPRPHDTGMVTMATQWQNEAHCNVLAHEELGLPTTPYRFAGSLEELRAGASEVGYPCVVKPVMSSSGHGQSVVRSADAIDAAWTEAQEGRRAADEGDVSRVIVEALAPLERELTVLTVSSSAGIVTCAPIGQRQESGDYRESWQPATEPDGEAERARDIARTAVEGLVAKAQAAGETGWGVFGVELFVLTDGSILFNEVSPRPHDTGMVTMASQRLSEFALHARAILGLPVSAQGPCVGVVMASRGYPLTSSKGDIISGLPSVTKGMVFHMGTKLNNYGEVVTNGGRVLIVVTTHQDLVLAAARATMACGRIIFNGAQFRTDIAHFCVGVVMASRGYPLTSSKGDIISGLPSVTKGMVFHMGTKLNNYGEVVTNGGRVLIVVTTHQDLVLAAARATMACGRIIFNGAQFRTDIAHRGFQTNQVGAILCVGVVMASRGYPLTSSKGDIISGLPSVTKGMVFHMGTKLNNYGEVVTNGGRVLIVVTTHQDLVLAAARATMACGRIIFNGAQFRTDIAH"
  },
  {
    "reference": "MGVEQAAGDAAPRPVAGSGFEAIERAIADLKAGRAVVVVDDEDRENEGDLIFAAEMATPELVSFMVRYTSGYICVALTESECDRLDLPPMHHTNADSFRTAFTVTVDAKAGITTGISASDRAHTIRLLADAGAGPGDLVRPGHVLPLRAREGGVLRRPGHTEAAVDLARLAGLSPAGALCEIVSQKDDGDMARGDELGVFAAAHDLTLITIADLIAYRRRFEKHVVRVAQARIPTEHGEFIAYGYDSLLDGIEHIAMVRGDIATPEGTGEDVLVRVHSECLTGDVMGSLRCDCGPQLDAALAAVAAEERGVVLYMRGHEGRGIGLLHKLQAYQLQEAGADTVDANLALGLPADARDYGIGAQILVDLGVKSMRLLTNNPDKRAGLEGYGLTITGRVPLPVRAHRENLRYLRTKRDRMGHDLPDLGDGALADGTGT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A key design requirement for the protein is the optimization of the Nucleophile; for GTP cyclohydrolase activity, Proton acceptor; for GTP cyclohydrolase activity to achieve high catalytic efficiency for GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.2. The GTP, D-ribulose 5-phosphate, Zn(2+), Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.3. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..4. The protein requires Zn(2+) to activate a critical catalytic site.5. The designed protein should be optimized for magnesium ion binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, GTP binding, zinc ion binding, manganese ion binding activity in order to improve riboflavin biosynthetic process.6. The designed protein have GTP cyclohydrolase II domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "VRVDMPTEYGHFELVAFKEKNNKAEHLALIKGDIGNGEPVLTRVHSSCFTGDILGSLRCDCGDQLHAAMQMVEKEGRGLILYMNQEGRGIGLINKLRAYKLQEQGYDTVEANLELGFGVDERDYGVGAQILRYLGATKLRLMSNNPRKRAGLNGYGIEIVETVTPEQDRDVVDIPTQFGNFTFAGYSDEIEHKEYIAVMKGEIKNKENVTVRLHSECLTGDVFGSRRCDCQEQLHRALHELEESGEGLIIYLRQEGRGIGILNKLKAYKLQDEGYDTVEANHKLGFSDDLRDYAVAAQIIKDLGIKSIILKTNNPKKIEGLEKYGVKVAGRVRVDMPTEYGHFELVAFKEKNNKAEHLALIKGDIGNGEPVLTRVHSSCFTGDILGSLRCDCGDQLHAAMQMVEKEGRGLILYMNQEGRGIGLINKLRAYKLQEQGYDTVEANLELGFGVDERDYGVGAQILRYLGATKLRLMSNNPRKRAGLNGYGIEIVETVTPEQDRDVRVDMPTEYGHFELVAFKEKNNKAEHLALIKGDIGNGEPVLTRVHSSCFTGDILGSLRCDCGDQLHAAMQMVEKEGRGLILYMNQEGRGIGLINKLRAYKLQEQGYDTVEANLELGFGVDERDYGVGAQILRYLGATKLRLMSNNPRKRAGLNGYGIEIVETVVPERDRDARAAEVVLPTPYGGDFKAIAYDNEVDNANHLALIKGEIDPEKPVLVRVHSECLTGDVFGSQRCDCGDQLHAAMQQIDREGAVVILYMRQEGRGIGLINKLKAYALQDEGHDTVEANEVLGFKADLRDYGIGAQILTELGVRQIRLMTNNPKKIVGLEGYGLKIVERV",
    "response#2": "TTRIPLQQHGDFNMTVFENDLDPAEHFALVKTPLFANQVPLVRIHSECITGDVFGSCKCDCGKQLESSLALIAAEGGVLVYLRQEGRGIGLANKLKAYALQEAGLDTVEANLQLGLPADDRDYAIAYQILKHLGIDTVRLITNNPSKIKALEEYGICVSERITTRIPLQQHGDFNMTVFENDLDPAEHFALVKTPLFANQVPLVRIHSECITGDVFGSCKCDCGKQLESSLALIAAEGGVLVYLRQEGRGIGLANKLKAYALQEAGLDTVEANLQLGLPADDRDYAIAYQILKHLGIDTVRLITNNPSKIKALEEYGICVSERIPVYDGTVREIEINLPTSFGDFNAIAYTNDLDGREHIALVKGDINPHEPTLVRVHSECLTGDVFGSHRCDCGPQLEAALKQIEEEGKGVLLYMRQEGRGIGLLNKLKAYKLQEEGYDTVEANHKLGFPADLRDYGLGAQILEDVGVRKMRLLTNNPRKIKGLHGYELELVERVPIYPQRGLGKVVDIPTQFGNFTFAGYSDEIEHKEYIAVMKGEIKNKENVTVRLHSECLTGDVFGSRRCDCQEQLHRALHELEESGEGLIIYLRQEGRGIGILNKLKAYKLQDEGYDTVEANHKLGFSDDLRDYAVAAQIIKDLGIKSIILKTNNPKKIEGLEKYGVKVAGRPLYRKGLGKVVDIPTQFGNFTFAGYSDEIEHKEYIAVMKGEIKNKENVTVRLHSECLTGDVFGSRRCDCQEQLHRALHELEESGEGLIIYLRQEGRGIGILNKLKAYKLQDEGYDTVEANHKLGFSDDLRDYAVAAQIIKDLGIKSIILKTNNPKKIEGLEKYGVKVAGR",
    "response#3": "MSEANFPTRFGAFRLYLFKDKLSGEEHIALIKGDIKGKKNVLVRVHSQCFTGDVLGSLRCDCRDQMEKALKIINDEGQGVFLYMRQEGRGIGLSNKVHAYHLQDLGYDTVEANCRLGFEPDLRDYGIGAQILSELGLSSIRLLTNNPKKIVGLEGYGLKVTERVPVRNVTPDGEVVRVDMPTKWGHFKLIAFHEKGTKNEHLALIKGSWEPGEPVLTRVHSSCFTGDIMGSMRCDCGEQLHTAMEMIEREGKGVLLYMNQEGRGIGLLNKLKAYKLQEEGMDTVEANLHLGFGMDDRDYGVGAQILRYLGITQLRLMSNNPRKRAGLLGYGLEIVETVEANFPTRFGAFRLYLFKDKLSGEEHIALIKGDIKGKKNVLVRVHSQCFTGDVLGSLRCDCRDQMEKALKIINDEGQGVFLYMRQEGRGIGLSNKVHAYHLQDLGYDTVEANCRLGFEPDLRDYGIGAQILSELGLSSIRLLTNNPKKIVGLEGYGLKVTERVPVEEVKREIEINLPTSFGDFNAIAYTNDLDGREHIALVKGDINPHEPTLVRVHSECLTGDVFGSHRCDCGPQLEAALKQIEEEGKGVLLYMRQEGRGIGLLNKLKAYKLQEEGYDTVEANHKLGFPADLRDYGLGAQILEDVGVRKMRLLTNNPRKIKGLHGYELELVERVPAANV"
  },
  {
    "reference": "MTANLKVLASVFKLRIGVFCALAAIAGALATPGAVPDFAPVMAVALAVLLSAAAAGAFNHYWERDIDPMMNRTRNRPFATGQFAAGPLWPLGLLALTVAAVALAAFAANAWAALHVFLGAFVYGIVYTVWLKRRTAWNIVIGGLSGSFAVLAGAAVAVPTLSPESLILALVLFLWTPPHFWSLATALKDDYAAAGIPMLPVVCSETETNRIILANTIALVASSLLPAFFGAGPLYLGAAILGGGWFLYKSVALVRRPGRKAAMGNFFASLIQLVLLLTAVMVEPLLAG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be optimized for activity in Porphyrin-containing compound metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "VGGGPGDPELLTLKALRLIQQADLIVYDRLVSPEVLNLARRDAERIYAGKKRSEHTLSQGSINNLLIKHAQLGKRVVRLKGGDPFIFGRGGEEIETLLEKKIPFEVVPGITAASGCASYAGIPLTHRDYAQSCVFVTGNLKENKVNLDWDQLAKPNQTIVIYMGLVGIHEVCRELIAHGLPKNTPAALIQKGTTVEHKVFISNLNELPSTVSKEKPKPPTLIIIGHVY",
    "response#2": "VGGGPGDPELLTLKALRLIQQADLIVYDRLVSPEVLNLARRDAERIYAGKKRSEHTLSQGSINNLLIKHAQLGKRVVRLKGGDPFIFGRGGEEIETLLEKKIPFEVVPGITAASGCASYAGIPLTHRDYAQSCVFVTGNLKENKVNLDWDQLAKPNQTIVIYMGLVGIHEVCRELIAHGLPKNTPAALIQKGTTVEHKVFISNLNELPSTVSKEKPKPPTLIIIGHVR",
    "response#3": "VGGGPGDPELLTLKALRLIQQADLIVYDRLVSPEVLNLARRDAERIYAGKKRSEHTLSQGSINNLLIKHAQLGKRVVRLKGGDPFIFGRGGEEIETLLEKKIPFEVVPGITAASGCASYAGIPLTHRDYAQSCVFVTGNLKENKVNLDWDQLAKPNQTIVIYMGLVGIHEVCRELIAHGLPKNTPAALIQKGTTVEHKVFISNLNELPSTVSKEKPKPPTLIIIGHVAG"
  },
  {
    "reference": "MEQGPQDGEPIEIKIIKEAYKKAFVFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISISSTDPEYTGPEWESARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQEVPKLYPEFPPKDMSEKSPEPQSLSSLPQHSEVNGSTSTASAESSSTPTTLSLPCQSHPSEAPPAYTPQAAEGHYTVSYGTESGEFSSVGENFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTFLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQASGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQTNWDRAEGENEFQIPGISGSASDQLKEASGTDVRQLDPSSKDVRQKGKRGKKTKGTSSEEVNLSHIVPCEPVSEEKAKELPEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTKGLYMAKQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDRNGKSTLDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHTAGEATHNAVDSAINVGVTAYNIDNIGIKAMVKKTAKQTGHTLLEDYKIIDNSKGENPGGGASANLKGEKDEQKEGPEKNGAKKKDK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: May be implicated in endosomal trafficking, or microtubule dynamics, or both; Participates in cytokinesis..2. The designed protein should contain one or more Senescence, MIT domains that are essential for its function.3. The designed protein's functionality will benefit from Basic and acidic residues, Polar residues compositional bias in its structure.4. The designed protein should have ubiquitin protein ligase binding within mitochondrial outer membrane, synapse, cytosol, lipid droplet, midbody to regulate negative regulation of BMP signaling pathway, abscission, negative regulation of collateral sprouting in absence of injury, cell division. The designed protein sequence is ",
    "response#1": "MAEISRIQYEMEYTEGISQRMRVPEKLKVAPPNADLEQEFQDGVPNASVIMQVPERIVVTGNNEDISFSRPADLDLIQSTPFKPLALKTPPRVLTLSERPLDFLDLERPLPTPQSEESRAVGRLKRERSMSENAVRQNGQLVRNDSIVTPSPPQARVCPPHMLPEDGANLSSARGILSLIQSSTRRAYQQILDVLDENRRPVLRGGSAAATSNPHHDNVRYGISNLDAAIEGASDDMTVVDAASLRRQIIKLNRRLQLLEEENKERAKREMVMYSITVAFWLLNSWLWFRR",
    "response#2": "VEDNCSLMYLNEATLLHNIKVRYSKDRIYTYVANILIAVNPYFDIPKFYSSDAIKKYQGRSLGTLPPHVFAIADKAYRDMKVLKMSQSIIVSGESGAGKTENTKFVLRYLTESYGTGQDIDDRIVEANPLLEAFGNAKTIRNNNSSRFGKFVEIHFNEKNSVVGGFVSHYLLEKSRICVQGKEERNYHIFYRLCAGAPEDIREKLYLSSPDSFRYLNRGCTRYFATKETDKQILQNRKSPEYLKAGSLKDPLLDDHGDFNRMCTAMKKIGLDDAEKLDLFRVVAGVLHLGNIDFEEAGSTSGGCTLKAQSQPALECCAALLGLDEEDLRVSLTTRVMLTTAGGAKGTVIKVPLKVEQANNARDALAKTVYSHLFDHVVNRVNQCFPFETSSFFIGVLDIAGFEYFEHNSFEQFCINYCNEKLQQFFNERILKEEQELYQKEGLGVNEVRYVDNQDCIDLIEAKLIGVLDILDEENRLPQPSDQHFTSVVHQKHKDHFRLSIPRKSKLAVHRNVRDDEGFIIRHFAGAVCYETTQFVEKNNDALHMSLESLICESKDKFVRQLFESNTNNNKDPKQKAGKLSFISVGNKFKTQLNLLLEKLHSTGSSFIRCIFPNLKMTSHHFEGGQILSQLQCSGMVSVLDLMQGGFPSRASFHELYNMYKKYLPEKLARLDPRLFCKALFKALGLNEIDYKFGLTKVFFH",
    "response#3": "MYGISSLDAAVEGASEDMSVVDAASLRRQIIKLNRRLQLLEEENKERAKREMVMYSITVAFWLLNSWLWFRR"
  },
  {
    "reference": "MLTGKRILLGVTGSIAAYKTASFVRLLIKAGAEVQVVMTQAAQDFITPLTLSTLSKRPVYTKLVANDTTGVWNNHVELGLWADLMIIAPATAHTLAKAAQGHCDDLLMAVYLSARCPVYWAPAMDLDMYHHPATRENLNRLQQFGNTIIQAGYGELASGLIGEGRMAEPEELVAVLENHFVQTPWKNKKVLITAGPTQEAIDPVRFISNHSSGKMGYALAGQLAAQGAQVTLVSGPTALASPHPSVTLRQIRSAQEMYEACREVFAEQEVTILAAAVADYTPTVVAEQKIKKTEASFTIELRKTVDIAATLGQQKRSDQLLVGFALETTNEVANAREKLIRKNFDLIVLNSLNHSGAGFGYDTNQITVIDRNQQLTDYPLKSKTEVASDILKAIAAYL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design should consider the endogenous proteins and enzymes present in the coenzyme A biosynthesis, Cofactor biosynthesis pathway to avoid interference with native metabolic processes.2. Mg(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "LYPGSFDPLTFGHLDLIQRGSDLFGEVLIAVLENPSKKATFSCERRIEQIKNATKDIPGCRTIAFKGLTVDCAHENNADLILRGLRAMSDFEYELQVAHTNRSLNNQYETIFLATETHHSFLSSSVVKS",
    "response#2": "EVVLGVGAGIAAYKVCDLLRRFTESGHGVTVVPTAEALRFVGEPTWAALSGRPVATGVWDGVHEVPHVRIGQRADLVFVAPATADLLARAAHGLAGDLLTNTLLTARCPVVFAPAMHTEMWEHPATQANVATLRSRGAIVLDPAVA",
    "response#3": "MRVVLGVAGGIAAYKACSLLRLLAEAGHDVTVVPTAAALKFVGAPTWAALSGKPVSTDVWDNVHEVPHVRLGQEADLVIVAPATADLLARAATGQADDLLTNVLLTARCPVVLAPAMHTEMWDHAATQANVATLTERGVHVVPPASGRLTGKDTGPGRLPEPEELYELLA"
  },
  {
    "reference": "MVQNIAILGASGYTGAELVRLIATHPAMRIVALSGDRKAGMAMSEVFPFLRHLDLPRLQKIEEIDFSSVDLAFCALPHATSQAVIAGLPRDLKVVDLSADFRLRDPAAYETWYGKPHAAPELQKEAVYGLTEFYRDEIRGARLVAGTGCNAATGQYAIRPLIEAGVIDLDDILIDLKAGVSGAGRSLKENLLHAELSEGTHAYSAGGRHRHLGEFDQEFSKIAGRPVQVRFTPHLTPMNRGILANVYVKGDPQAVHRALTERYLTETFLEVLPFGALPSTRDIRGSNYVHIGVIGDRVPGCAMVVAVLDNLCKGSSGQAIQNANLMLGLDEAAGLGLAPVFP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should consider the endogenous proteins and enzymes present in the N(2)-acetyl- L-ornithine from L-glutamate: step 3/4 pathway to avoid interference with native metabolic processes.2. The protein must be able to protein dimerization activity, NADP+ binding.3. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.4. For general function, the protein need meet that Catalyzes the NADPH-dependent reduction of N-acetyl-5- glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde. The designed protein sequence is ",
    "response#1": "MKVFVDGQEGTTGLRLLDYLSQRDDIELLRIAEDRRKDPAERAKFLNAADVAFLCLPDVASREAVSLVDNPNTCIIDASTAFRTSDDWAYGLPELAPGQRERLIAGPGCNVTATTLALQPLVAQGLVDVDAIVVDPSGYSGAGRQPSGRSLHSEVANAMRAYKVGGHHRHVPEIEQGLERAAGRAVRVTFTPHLMPMIRGMQSTIYARLRDGLSGAELHARYREFYAGEPFVRVLPAGEHPETRWVRGSNFCDIGLTVDPRTGRLVVLSVIDNLMKGAAGQAVQNMNLMFGLEETLGLDFLPLFP",
    "response#2": "SVGIIGATGYVGTEIVRLLQNHPDINITSVVSHNFAGQKISDIYPNLKNVFEMECDELDIDKIADKAEVFVTALPHGISKEVIPKLVEKGKRIVDHSGDFRYKSVEVYEKWYNATHGMPHLLKLSAYGLPELHREEIKNIAGPGCNVTATTLALQPLIENNLIDVSSIIIDAKSGV",
    "response#3": "RIAIIGASGYGGLQLVKLINEHPDFEISTLNGERSVGKNWNEINPFMKILGDKKITKSNIDEIALDSDYAILSLPNGLSSQLTPLLLKKGVKVLDLSADYRFKSLDKWKEVYTKEAAKYKRYDYDLCEEAIYGFSEEFSSEIKYSKGLIACPGCYPTAVQLGFLPALKENLIDDSIIVDAKSGVTGAGKKPGAATLFAEVNESFKAYKVGGTHRHTPEIEQELSRVAGQDVTVKFTPHLLPMIRGMLSTIYARTKGPVSEEALRALYRGFYADEPFVRVLPAGSHPETRGVMGSNFCDIGVEVDSRTGRVIVISAIDNLVKGAAGQAIQNMNLMFGLPESAGLEFTPVYP"
  },
  {
    "reference": "VAQRSAVGRGGGKSRWQGPRAGGREPQGVGGTSSRTFLWPRLRSEPGKGRGRGTAGQGEGGRRAPPGLQAPPPDDVDYATNPHVKPPYSYATLICMAMQASKATKITLSAIYKWITDNFCYFRHADPTWQNSIRHNLSLNKCFIKVPREKDEPGKGGFWRIDPQYAERLLSGAFKKRRLPPVHIHPAFARQAAQEPSAVPRAGLLTVNTEAQQLLREFEEATGEAGWGAGEGRLGHKRKQPLPKRVAKVPRPPSTLLPTPEEQGELEPLKGNFDWEAIFDTGTLGGELGALEALELSPPLSPASHVDVDLTIHGRHIDCPATWGPSVEQAADSLDFDETFLATSFLQHPWDESGSGCLPPEPLFEAGDATLASDLQDWASVGAFL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must have at least one Fork-head domain.2. The ideal protein would be one that plays a critical role in positive regulation of lung ciliated cell differentiation, motile cilium assembly, actin cytoskeleton organization, lung epithelium development, negative regulation of T cell proliferation through RNA polymerase II cis-regulatory region sequence-specific DNA binding, DNA-binding transcription activator activity, RNA polymerase II-specific within nucleus.3. A protein with Fork-head capability for targeted gene regulation. The designed protein sequence is ",
    "response#1": "RPPYSYSALIAMSIQNATDKRLTLSQIYQYVAENFPFYKKSKAGWQNSIRHNLSLNDCFKKMPRDENDPGKGNYWTLDSNCEKMFDNGNFRRKRLWKNSIRHNLSLNKCFRKNNKPPYSYAQLIVQAISAAPDKQLTLSGIYSFIVKHYPYYRKETNKGWQNSIRHNLSLNRYFIKVARSQDEPGKGSFWRIDPDSGAKLIDHSYKKRRQR",
    "response#2": "NMRPPFTYATLIRWAILEAPEKQRTLNEIYHWFTRMFAFFRNHPATWKVSSSEVAVAGMASSAIAAQSGQAWVWAHRHTGEERDVGCWWWLLASEVDAHLLPVPGLPQNAIRHNLSLHKCFVRVESEKGAVWTVDELEFRKKR",
    "response#3": "NMRPPFTYATLIRWAILEAPEKQRTLNEIYHWFTRMFAFFRNHPATWKVSSSEVAVAGMASSAIAAQSGQAWVWAHRHTGEERDVGCWWWLLASEVDAHLLPVPGLPQNAIRHNLSLHKCFVRVESEKGAVWTVDELEFRKKR"
  },
  {
    "reference": "MYPMDSDYHRRGLVANDRSPAQFVRLDKPRAVDDLYIGKREKMRRWLCCACHVEEPYHSSENEHLRSPKHHNDFGHHTRKPQAAVKPDALKEPPSIDVPALSLDELKEKTDNFGSKSLIGEGSYGRAYYATLKDGKAVAVKKLDNAAEPESNVEFLTQVSRVSKLKHDNFVELFGYCVEGNFRILAYEFATMGSLHDILHGRKGVQGAQPGPTLDWIQRVRIAVDAARGLEYLHEKVQPAVIHRDIRSSNVLLFEDFKAKIADFNLSNQSPDMAARLHSTRVLGTFGYHAPEYAMTGQLTQKSDVYSFGVVLLELLTGRKPVDHTMPRGQQSLVTWATPRLSEDKVKQCVDPKLKGEYPPKAVAKLAAVAALCVQYESEFRPNMSIVVKALQPLLRSSTAAAVPVQEA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be optimized for cytosol, plasma membrane-specific folding and stability, while maintaining non-membrane spanning protein tyrosine kinase activity. The designed protein sequence is ",
    "response#1": "KNRYKDILPYDHSLVELSLLTSDEDSSYINASFIKGVYGPRAYIATQGPLSTTLLDFWRMIWEYRVLVIVMACMEFEMGKKKCERYWAEPGETQLQFGPFSISCETEKKKSDYKIRTLKAKFNSETRIVYQFHYKNWPDHDVPSSIDPILQLIWDMRCYQEDDCVPLCIHCSAGCGRTGVICAVDYTWMLLKDGIIPKNFSVFNLIQEMRTQRPSLVQTQEQYELVYSAVLELI",
    "response#2": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPR",
    "response#3": "NIKKNRYKDILPYDHSLVELSLLTSDEDSSYINASFIKGVYGPRAYIATQGPLSTTLLDFWRMIWEYRVLVIVMACMEFEMGKKKCERYWAEPGETQLQFGPFSISCETEKKKSDYKIRTLKAKFNSETRIVYQFHYKNWPDHDVPSSIDPILQLIWDMRCYQEDDCVPLCIHCSAGCGRTGVICAVDYTWMLLKDGIIPKNFSVFNLIQEMRTQRPSLVQTQEQYELVYSAVLF"
  },
  {
    "reference": "MQGKPRIAVIGAGLGGTAGAALMARAGFNVRLYEQAPAFSRLGAGIHLGPNVMKIMRRIGIEDELNRQGSHPDYWYSRDWQSGAELARIPLGDYAVSHYGATYLTVHRGDFHALMTAALPAGLLQFNKRLTRVDEDDDVVRLHFADGSVEEAEIVIGADGVNSRLREHLLGAELPKYTGYVAHRAVFPTPLDSGSLPFDMCVKWWSDDRHMMVYFVTGKRDEIYYVTGVPEQQWDMGKSWVPSSKAEMRAAFAGWHPTVQALIEATPEVSKWPLLERDPLPLWSRGRIVLLGDACHPMKPHMAQGAAMAIEDAAMLTRIFEQTGLQDHAAAFRLYEDNRAERASRVQRVSHDNTWLRTNENPDWCFGYDVYAEPLVEGRRAAA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Proton acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.2. The protein must be engineered to have a high affinity for FAD in order to increase its catalytic activity.3. The designed protein must be optimized for 6-hydroxynicotinate 3-monooxygenase activity.4. The protein must exhibit the following characteristics: Flavin-dependent monooxygenase (FMO) that catalyzes the decarboxylative hydroxylation of 6-hydroxynicotinic acid (6-HNA) to 2,5-dihydroxypyridine (2,5-DHP) with concomitant oxidation of NADH, a step in the aerobic nicotinate degradation pathway. The designed protein sequence is ",
    "response#1": "MASLSAAVNIITTEGDAGQCGITATAVCSVTDTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSCWQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFHD",
    "response#2": "MASLSAAVNIITTEGDAGQCGITATAVCSVTDTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSCWQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFHPR",
    "response#3": "MASLSAAVNIITTEGDAGQCGITATAVCSVTDTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSCWQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFHPG"
  },
  {
    "reference": "MRAKGTRKNYQHLWRWGIMLLGMLMICSATEKLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPSDPNPQEVVLGNVTENFNAWKNNMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDVEPTKRNTNNTGNTTTTTSSPATSSNGEMMETGEIKNCSFNITTSMKDKVQKTYALFYKLDVVPMEDDKNTSYRLISCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNDKNFNGTETCANVSTVQCTHGIRPVVSTQLLLNGSLAEKDVVIRSENITDNAKTIIVQLNETVEINCTRPNNNTRKSIPIGPGRAFYTTGEIIGDIRKAHCIINKTKWVNTLEKVAKKLRETFNKTIVAFNQSSGGDPEIVMHSFNCGGEFFYCNSTGLFNFTFAENGTRESSNINKTGNITLPCRIKQFINLWQGVGKAMYAPPIQGQISCSSNITGLLLTRDGGNNNETNRPETFRPGGGDMKDNWRSELYKYKVVKIEPLGVAPTRAKRRVVQREKRAVTLGAMFLGFLGAAGSTMGAASMTLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTAVPWNTSWSNKSYSQIWDNMTWMEWEREIDNYTSLIYSLIEESQNQQEKNEQELLELDKWSNLWTWFSISNWLWYIRIFIMIVGGLVGLRIVFTVISIVTRVRQGYSPLSFQTRLPARRGPDRPEGIEEEGGERDRDRSGRLVTGFLALI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The ideal protein would be one that plays a critical role in positive regulation of receptor clustering, positive regulation of plasma membrane raft polarization, fusion of virus membrane with host plasma membrane, viral protein processing through structural molecule activity within plasma membrane, virion membrane, host cell plasma membrane, viral envelope.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.4. The protein design must feature the YXXL motif; contains endocytosis signal motif.5. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.6. The protein must exhibit the following characteristics: The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane. The designed protein sequence is ",
    "response#1": "MRVMGTQRNYPPFWRWGMIIFWIMMICNAKDLWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQEMVLGNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLRCNNTNNNTNNTNTNATINNTTNITTNEELKNCSFNVTTELRDKRRKEYALFYKLDVVPLGGSPSEYRLINCNSSTITQACPKVSFDPIPIHYCAPAGYAILKCNNKTFNGKGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEKEIIIRSENLNDNGKTIIVHLNESINIVCTRPNNNTRKSIRIGPGQAFFATGEVIGDIRKANCTINESKWNNTLRQVKDKLLEHFPNKTIIFNSSAGGDLEVTTHSFICRGEFFYCNTSGLFNRTYSRNNDTNSADSTSNSTITIPCKIKQIVNMWQEVGRAMYAPPIKGSITCNSTITGLLLTRDGGNTTGNTTETETFRPGGGNMRDNWRSELYKYKVVEIKPLGIAPTEAKRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQHHLLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKSQTDIWDNMTWMQWDKEISNYTNTIYRLLEESQSQQERNEKDLLALDNWNSLWNWFNITNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTLLPTQRGPDRPEGI",
    "response#2": "MWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPIDPNPQEVVLENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLKCSNLNITNSKLDNNTSTNDTATNNSSIGEGMEGGEMKNCSFNVTTSIRDKVKKEYALFYRLDVEQIDDTDNTSYTLINCNSSVITQACPKVSFEPIPIHYCTPAGFAILQCNDKKFNGTGPCSNVSTVQCTHGIKPVVSTQLLLNGSLAEEEMIIRSENFTNNAKTIIVQLNASVEINCTRPNNNTRKGIHVGPGRMIYATRRIIGDIRQAHCNISREKWNNTLTQLVVKLREQFGNNKTIVFTQPPSGGDPEIVMHSFNCGGEFFYCNTTQLFNSTWIGNSTPGLDGTENITLPCRIKQIINRWQEVGRAMYAPPIEGQIRCSSNITGLLLTRDGGTKNNKNKENETFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTEARRRVVQREKRXXXXXXXVHARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARILAVERYLQDQKLLGLWGCSGKIICTTAVPWNSTWSNKTYEDIWGNMTWVQWEKEINNYTGQIYELLAESQNQQDKNEKDLLELDKYNALVGINL",
    "response#3": "MWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPIDPNPQEVVLENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLKCSNLNITNSKLDNNTSTNDTATNNSSIGEGMEGGEMKNCSFNVTTSIRDKVKKEYALFYRLDVEQIDDTDNTSYTLINCNSSVITQACPKVSFEPIPIHYCTPAGFAILQCNDKKFNGTGPCSNVSTVQCTHGIKPVVSTQLLLNGSLAEEEMIIRSENFTNNAKTIIVQLNASVEINCTRPNNNTRKGIHVGPGRMIYATRRIIGDIRQAHCNISREKWNNTLTQLVVKLREQFGNNKTIVFTQPPSGGDPEIVMHSFNCGGEFFYCNTTQLFNSTWIGNSTPGLDGTENITLPCRIKQIINRWQEVGRAMYAPPIEGQIRCSSNITGLLLTRDGGTKNNKNKENETFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTEARRRVVQREKRAALGMLFLGVLSAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQTLLGLWGCSGKIICTTAVPWNSTWSNKSFEEIWDNMTWTEWEREISNYTNQIYDILTASQNQQDRNEKELLALDKWTSLWNWFDITNWLWYIKIFIIIVGSVIGLRIIFAVLSIVNRVRQGYSPVSFQTPTHQREPDRPEGIEEGGGEQDRDRSVRLVSGFLALAWDDLRSL"
  },
  {
    "reference": "MNTDAQSTSNTRNFMYSPDSSLEVVIITNSDGDHDGYLELTAAAKVMSPFLSNGSSAVWTNAAPSHKLIKNNKNYIHVFGLFKYLSNYNLNNKKRPKEYYTLKSIISDLLMGAQGKVFDPLCEVKTQLCAIQESLNEAISILNVHSNDAAANPPAPDINKLQELIQDLQSEYNKKITFTTDTILENLKNIKDLMCLNK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein localizes to the host cell cytoplasm, host cell nucleus, viral capsid. The designed protein sequence is ",
    "response#1": "MPIGVPKVPFR",
    "response#2": "MP",
    "response#3": "MPRGRCWICNIEGHYANECPR"
  },
  {
    "reference": "MKQLEDKIREEGRALSDQVLKVDAFLNHQVDPVLMQAIGKEFAERFKDAQIDRIVTLESSGIAPAMMTALEMDIPFVFARKRKSLTLQDDLVEADVYSFTKQETNRISLSRRFVLPGERVLVIDDFLANGEAALGLTQLVEAAGAEVSGVGIVIEKSFQLGRDKLIERGYRVESLARVAKLEKDHISFVEVVR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Ideal protein should have a binding affinity for key enzymes in the XMP biosynthesis via salvage pathway, Purine metabolism, XMP from xanthine: step 1/1 pathway to enhance the overall efficiency of the pathway.2. The ideal protein would be one that plays a critical role in xanthine metabolic process, XMP salvage, purine ribonucleoside salvage through xanthine phosphoribosyltransferase activity within cytoplasm.3. The protein should be able to bind xanthine, 5-phospho-alpha-D-ribose 1-diphosphate ligand in a variety of conditions4. We need a protein that can perform diphosphate + XMP = 5-phospho-alpha-D-ribose 1-diphosphate +   xanthine with high efficiency.5. For general function, the protein need meet that Converts the preformed base xanthine, a product of nucleic acid breakdown, to xanthosine 5'-monophosphate (XMP), so it can be reused for RNA or DNA synthesis. The designed protein sequence is ",
    "response#1": "MGTEFAHLFQDAGVTKILTVESSGIAPAVMAGLAMHVPVVFARKHKSVTLIDDLYTAEVYSYTKKTSNHISIAKKFLQADDQVLLIDDFLANGQAVQGMFEICDKAHVKIAGVGIVIEKVFQTGHQLIADRGVRLESLLKRGHEIKWVCKKDMIGKEFGRLFKKQNVTKILTVESSGIAPAVMTGLAMDVPVVFARKHKSLTLTDDLYTASVYSYTKQTSNQISISKRFIDEKDRVLIIDDFLANGQAVSGLLDIADAANIDVVGVGIVIEKTFQKGSQIIKDRMTMLTAGKGLRGVIRIEEGPYFKPSSPQRADLVEPEGKARLVELSGQVFTRSCRPVGQALVDLWHADERGQYDNAGFRYRGHLFTDEEGRYRFRTILPALYTGRTRHYHVKVQAPRQNLLTTQLYFPDEPMNRRDGLFRAVLYR",
    "response#2": "MREIGKVFAEKFKDTGITKVVAIEASGIAPALYATEALDVPMIFAKKAKNITMNEGILTAEVYSFTKQVTSTVSIASKFLTPEDKVLIVDDFLANGQAAKGLIQIIEEAGAHVEAVGRGVAVVSVEDEEGQSVGLMEGPYFKPSSPQRADLVEPEGKARLVELSGQVFTRSCRPVGQALVDLWHADERGQYDNAGFRYRGHLFTDEEGRYRFRTILPALYTGRTRHYHVKVQAPRQNLLTTQLYFPDEPMNRRDGLFRAVLEQ",
    "response#3": "MGTEFAHLFQDAGVTKILTVESSGIAPAVMAGLAMHVPVVFARKHKSVTLIDDLYTAEVYSYTKKTSNHISIAKKFLQADDQVLLIDDFLANGQAVQGMFEICDKAHVKIAGVGIVIEKVFQTGHQLIADRGVRLESLGVDLWVYDPENADPALATALAARLPLVYAKKYDPDVPVPALTRHIHSPTRDRQVQLAISARFIPAGSRVAIVDDFLANGRTALALADMVYEAGATVVAAAFVVEKPDHGRRLPEALREGPYFKPSSPQRADLVEPEGKARLVELSGQVFTRSCRPVGQALVDLWHADERGQYDNAGFRYRGHLFTDEEGRYRFRTILPALYTGRTRHYHVKVQAPRQNLLTTQLYFPDEPMNRRDGLFRAVIEVLG"
  },
  {
    "reference": "MFKVNEYFDGTVKSIAFEGTEGPATVGVMAPGEYEFGTAKREIMHVVSGALTVKLPGSDNWETFNAGDKFNVAADSKFQLKVAVDTAYLCEYRD",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Catalyzes the phosphorolysis of diverse nucleosides, yielding D-ribose 1-phosphate and the respective free bases.2. The protein must exhibit uridine phosphorylase activity. The designed protein sequence is ",
    "response#1": "MTP",
    "response#2": "MAFLS",
    "response#3": "MCLL"
  },
  {
    "reference": "MASIMIGSITCALPTSAKECANTYAHPNVVTQRTKHEMLSDYSKRELEKYASTQASIWSALEAKLESSLNNRVDKTVHCTAKGLVKLKAPTARIRRDRARKARQELETEEFLRSPPTVITSLSIAGGRQASAMEADKEQRGTVHALSRRRTSRQRKQVCLSKVGLDNLIKEVARMQHKRSLPVEVIGKQSVKTYSKTVNGRKFITVRTHHEDGKIRKIDMPHSTFINETLRAMADARQYTNVDTDELSFGSSGIIIPMARAHGNCGRFWNGICIVRGRCDGRLFDARSILSRSVYMHMDHYSDPGTAFWRGFNRSFLSHRPSFLDHSCMSDFDVETCGVVAALVCQAILPCGRITCTQCADNNVSVIDKMKLDSMSENILKTSDLIERSYPQFRHVVSFLKKHNKFLNSFNTNTEAFGKIKFIIGERTDAPFSHILRMNEILIKGGEATTSELSEGTNLLLELARYHKNRTENIRQGSLQSFRNKVSAKAHVNTSLMCDNQLSKNGSFIWGERGYHAKRVFSNYFEQINPEDGYNKYIIRVNPNGSRKLAIGKLIVSTNFETLRQQMKGEPVEAQPITNSCVSKRDENFVYPCCCVTLDDGTPLYSDVKMPTKNHLIIGNSGESKILDLPTETGLAMYIAKDGYCYVNIFLSMLVNVVEEEAKSFTKMVRDVVVEKLGKWPSMLDVATACYLLTVFHPTTKNAELPRILVDHTTRTMHVIDSYGSLNTHYHVLKANTVSQLILFASDSLESELKHYTVGGLQNEMQAR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should contain one or more Helicase C-terminal domains that are essential for its function.2. The designed protein should be optimized for RNA-dependent RNA polymerase activity activity in order to improve RNA-protein covalent cross-linking, viral RNA genome replication, proteolysis. The designed protein sequence is ",
    "response#1": "SPTKQIHIPGKIDSILHMAMIESFIPINNIPDHIGKIPIYSIQITRKVNDRNILILPLEMDNTLFSSTLLGEVLNYFSNWSGSITITFMCVCDSFSTGKFLVAYTPPGGKPPTDRKDAMLGVHIVWDLGLQSSCSIVVPWMSAGFFRHTMKDKYSSGGYITLQ",
    "response#2": "NPYAIAPDAADTLEKYGIATNPFAVKVHSHGAVKSIENTLLERVGFNLPKEPCTFLFLKRSKLRYLRRGPSNNDIFINLAIEPRDLQRYEEDTLVESWTRITTRYAYISDTFHFFTRKMLADLFFHNPALDVLYATLVLPPEALHKHPSIEPDLYTINYNFNGFQYIPGNHGGGSYSHEFKQLEWLKVGHLKSPELCLTFQMIESIGANHLFMITRGIKITPRVRTFTKDSYVLFPQIFHPRNLNPSKPFPKVKAMQLFTYVKSVKNPTERDIYAKIRQLIG",
    "response#3": "SFNQKSYSNHGVFNAMRACENFYFSRKFKNSDYIDAGGDVVSTLRSKNHNVHICSPRLDLKDAARHIQRATVIDGLKGYGETISFCTNKTEDCAVNRDIIIAVEVYDMTLRDMAKAMLSHGSRKFEFSCIIPPEIFTKECNVELYEGRLKVTRIGDNVEYYYGSNGETFSHSCQTLKDIC"
  },
  {
    "reference": "MSKVIGIDLGTTNSCVAVMEGGEAVVIPNAEGGRTTPSVVAFTKEGERIVGQVAKRQAITNPDRTVISIKRHMGTNYKVKIDNKEYTPQEISAMILQKLKADAEAYLGEKVTQAVITVPAYFTDSQRQATKDAGRIAGLEVLRIINEPTAASLAYGLDKGEDQTILVYDLGGGTFDVSILELGDGVFEVKATSGNNRLGGDDFDQRIMDYLVDICRREHGVDLTQDKMAMQRLKEAAEKAKIELSGMTSTNINLPFISATPNGPVHLDVNLTRAKFEELIADLVEKTVGPTRQALADAGLEPKDIDKVLLVGGSTRVPLVQETVRKILGQEPHKGINPDECVALGAAIQGGVLAGEVKDVLLLDVTPLSLGIETLGGVFTKLIERNTTIPTSKSQIFSTAADNQTTVEIHVLQGERAMAADNKTLGRFQLTGIPPAPRGVPQIEVKFDIDANGIVHVSAKDLGTGKQQAITITSSSGLSEEEIQRMVKEAEASAEADRRRKEEIETRNQADSLIYQAERTLKEFKDKADQNDVDRIEKAKKELQEVLDSKNNDKIKEKMEALSQALYTLTTKVYQQAGAQAGAQGQGAAGGQKQDGNVYDADYKVVDDDKKE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must have high specificity towards ATP-dependent protein folding chaperone, ATP binding, unfolded protein binding.2. For general function, the protein need meet that Acts as a chaperone. The designed protein sequence is ",
    "response#1": "ENIFPIIKKFLYSDHEIFLRELVSNAVDATQKLNTLASISEFKGELGDLTVHVSLGKDTITISDRGIGLTAEEIDKYINQIAFSGANDFLEKYKNDANAIIGHFGLGFYSAFMVSKKVEIITKSYKEGAQAVKWTCDGSPEFTLEEVEKADRGTDIVLYIDDDCKEFLEESRISALLKKYCSFA",
    "response#2": "ENIFPIIKKFLYSDHEIFLRELVSNAVDATQKLNTLASISEFKGELGDLTVHVSLGKDTITISDRGIGLTAEEIDKYINQIAFSGANDFLEKYKNDANAIIGHFGLGFYSAFMVSKKVEIITKSYKEGAQAVKWTCDGSPEFTLEEVEKADRGTDIVLYIDDDCKEFLEESRISALLKKYCSFR",
    "response#3": "GENIFPIIKKFLYSDHEIFLRELVSNAVDATQKLNTLASISEFKGELGDLTVHVSLGKDTITISDRGIGLTAEEIDKYINQIAFSGANDFLEKYKNDANAIIGHFGLGFYSAFMVSKKVEIITKSYKEGAQAVKWTCDGSPEFTLEEVEKADRGTDIVLYIDDDCKEFLEESRISALLKKYCSFK"
  },
  {
    "reference": "MRVRGIQRNWPQWWIWGILGFWMLMTYNEAERLWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQEMVVNVTENFNMWDNDMVDQMHEDIVSLWDQSLKPCVKLTPLCVTLKCGNVTENSTVSNNTMEMKNCSFNVTTEIRDKEKQVYSLFYKLDIVPLDTENNVTNGTKEYRLINCNTSTITQACPKVSFDPIPIHYCAPAGYAILKCNKKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIVIRSKNLTDNTKTIIVQLNTPVEIVCTRPGNNTRQSIRIGPGQTFYATGDIIGNIRQAHCNISQEKWNKTLQAVGGKLREHFPNKTITFKPHAGGDLEITTHSFNCRGEFFYCNTSRLFNGTYDSTYNSTGNNTILTIPCRIKQIINMWQKVGRAMYAPPIAGNITCNSSITGLLLTRDGGTGTNGTEIFRPGGGDMRDNWRSELYKYKVVEIKPLGIAPTAARRRVVEREKRAVTLGAMFLGFLGAAGSTMGAASITLTAQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGLWGCSGKLICTTNVPWNGSWSNKTYTEIWDNMTWMQWDREINNYTYTIYNLLEISQNQQEKNEKDLLALNSWQSLWNWFDISNWLWYIKIFIMIVGGLIGLRIIFAVLSLVNKVRQGYSPLSFQTLTPNLREQPDRRERIEEGGGEQDRGRSIRLVNGFLALAWDDLRSLCLFSYHQLRDFILVT",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Include a signal peptide as an essential component of the protein.2. The protein must exhibit the following characteristics: Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm..3. The protein contains novel Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains that confer a unique function or activity.4. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.5. The designed protein should contain Cytoplasmic topological domains.6. The protein design must feature the Di-leucine internalization motif motif.7. The protein should be optimized for stability and solubility when expressed in virion membrane, viral envelope where it will be involved in positive regulation of establishment of T cell polarity. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWRDAETTLFCASDAKAYEREAHNVWATHACVPTDPSPQEVHLENVTEEFNMWKNNMVEQMHMDIINLWDQSLKPCVKLTPLCVTLNCSN",
    "response#2": "LWVTVYYGVPVWKEATTTLFCASDAKAYDTEAHNVWATHACVPTDPNPQEVELTNVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDWKCXNDSXXXXXXXXXXXXXXXXXXXGEIKNCSFNITTGISGKFQKEYALFHKLDVEPIXNDNASYRVISCNTSVITQACPKISFEPIPIHYCAPAGFALLKCNXKTFDGXGXCTNVSTVQCTHGIRPVXSTQLLLNGSLAEEXXIIRSANFSDNAKTIIVQLXTSVXINCTRPXNNTRKSIHLGQGRAWYTTGEIIGNIRQAXCNISKXEWTNTLXXVVXKLREQFKNKTIVFNQSAGGDPEIVMHTFNCGGEFFYCNTTQLFNTXWNXTXERINNTGXNETIILPCRIKQFINMWQQVGKAIYAPPIRGLIRCSSDITGLILTRDGXNNGNETNEVFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRLLLGMLFPPQEEHRRRARERTXELXAAXLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNIWATHACVPTDPNPQEVVLGNVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVILNCTNYSGNNTRNRXVXLPXXTHXAXYXDNTSEWEGEMKNCSFNVTTGIEGKKKTEYALFYTLDIIPIDNTSYMLKNCNTSVISQACPKVSFEPIPIHYCTPAGFAILKCNDKQFNGSGPCKNVSTVQCTHGIRPVVSTQLLFNGSLAEEEVMIRSENFTDNAKTIIVQLNGSVAFNCTRPNNNTRKSIHIGLGRAFYATGDIIGDIRQAHCNLSRAKWNDTLKMIVSKLKEQFKNKTIIFNSSSGGDPEIVMSTFNCGGEFFYCNTTQLFNSTWLXNGTGNSTTGNITLPCRIKQIINRWQEVGKAMYAPPISGEISCSSNITGLLLTRDGGKPGNAAESINETFRPGGGNMKDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQGEK",
    "response#3": "GNNTRNNTSNTLEGGEMKNCSFNITTSIGTKVTDSAIFNKLDIIPIEGNSSNYRLINCNTSVFTQACPKVSFEPIPIHYCTPAGYAILKCNNKTFNGKGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEGVVIRSSNFTNNAKVIIVQLNESVAIDCTRPNNNTRKSIHLGPGRALYMSGGIVGNIRQAHCNISGTKWNDTLRQITGKLREQFGNKTIIFNQPAGGDPEIVMHSFNCGGEFFYCNTSQLFNSVNGTWNGTGTGNNTITLPCRIKQIVNMWQEVGKAMYAPPISGQIRCLSNITGLLLTRDGGNNGSNTTETFRPGGGDMRDNWRSELYKYKVVQIEPLGVAPTRAKRRVVQREKRXLGGLGAIFLGFLGVAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGMWGCSGKLICTTAVPWNSSWSEKNQTEIWDSLTWMQWDREISNYTDTIYRLLEDSQNQQESNEKDLLALDSWNNLWNWFNITNWLWYIKIFIMIVGGLIGLRIIFAVLALVNRVRQGYSPLSLQTLLPTQRGTDRPEGIEEEGGERDRDRSIRLVNGFLALAWDDLRNLCLFSYHRLRDFILIV"
  },
  {
    "reference": "MAARCAVCGKGPQTGYTVSHSHIRNKRRFLPNLQPVRTTVDGQNVRLRVCTKCLKAGKVQRVKVA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be involved in translation by localizing to ribosome and exhibiting structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "GRITLTSRNVRSLENVCRDLINGAKNQNLRVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRIIDLHSPSEIVKKITSINIEPGVEVEVK",
    "response#2": "DIERGIRVAVDTGKVILGSNKAIQAIKLGNGELAIMAENTPKNVKADIEAYSKLSEMPVYTFEGSSVELGSICGKPFTVSVLIIQEPGDSNIL",
    "response#3": "DIERGIRVAVDTGKVILGSNKAIQAIKLGNGELAIMAENTPKNVKADIEAYSKLSEMPVYTFEGSSVELGSICGKPFTVSVLIIQEPGDSNIL"
  },
  {
    "reference": "MTTTRNAARGVPAGAPRQRTVAASAVVTGKGLMLGREATLTIMPAPPDHGIVFERTDLERPVIIPALVSSVIPNARRTTLKAGDVTIETVEHCMSALRGLGIDNVLLKLHGPELPCGDGSALRFVDAIRAAGIAEQDAPRRMFKLMETVSVEEGDASIAAIPADSPGGMRLMYDLDYGANSTRIPHQAWSFDPARDDYVAEVAPARTFSLKEEAEALWQAGLCRHLTPRDVLVIGDEGPIQNEYRFPDEPVRHKMLDAIGDLALVGTIVECQVVANRSGHGLNRQLGMKLLRIVQRERQSRKLIEGRAMDARAIGRLMPHRFPMLLVDRVLELEPGRRAIGVKNVTINEPFFQGHYPGTPIMPGVLIVEALAQMGGLLLSQTLEHTGKVAVLLSLDKVKLRKAVTPGDQLVLEVETMRATSRSAAVKARALVGEKVAAEAEIRFMMVDQDFQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should take into account the physiological conditions of the Glycolipid biosynthesis, lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 2/6 pathway.2. A protein that can perform metal ion binding, (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity, UDP-3-O-acyl-N-acetylglucosamine deacetylase activity, 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity, 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity in cytoplasm to support fatty acid biosynthetic process, lipid A biosynthetic process.3. The protein must exhibit the following characteristics: Involved in unsaturated fatty acids biosynthesis; Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.. The designed protein sequence is ",
    "response#1": "MKLNTVLRGAGLEYQGSADVAVLGVTHDSRKVTEGQLFAALPGHHVHGAAYAEEVLAAGAVAVVSERPCELPAEFPGAPCLVVENTRVAASQLAANWYDQPSQNMTVVGV",
    "response#2": "MSNPYAXAXDXPXSXVPXXAXLXKXAXTAXGXSTXXDXEKPXIHAXIT",
    "response#3": "MSEFSQIASLKQTINQAQKTRPLAMLTAYDYPMATLLDEAGIPVLLVGDSLGMVLLGHESTLSVSLADMVYHTKAVKRGSPNAFLVADLPFLSYQTSVSEAVFNASRLMQEGGAQAVKLEGGGRVADTIKFLVERGIPVMGHIGLTPQSVISLGGHRVQGKEDAAAREVIADAQALDQAGVFSIVLEMVPAALAKEITQAISIPTIGIGAGVHTDGQVLVLHDILGLNEKFTPKFVKKYADLGPQISAALKQYTLEVKEGVFPSAEHSFHI"
  },
  {
    "reference": "MSSSKVKLTTATAIRQKNNTTVLDEARQESDSRRAFCFYLVNFTPLDHDHSNSHKLNLKGKKILLGISGSIAAYKSALLVRLLVKAEAEVRVVMTQSATEFITPLTLSTLSKNPVLTSFTKNETGEWNNHVELGLWADLIIIAPATAKTLSKCASGHCDDLLTAIYLSARCPVFFAPAMDVDMFKHPSTQHNIEKLVSYGNSFISPEHGELASGLIGDGRLAEPENIVKILDNHFAKRPVAGSRRVLITAGPTVEPLDPVRFISNRSSGKMGYAIAEAFAAAGAHVTLVSGPTHLKTSNPNIKTIAVETAGQMFQAAKDHFEANDLIIFAAAVADYTPRYVAEQKIKKQGDSMVLDLAKTTDIAGTLGAMKRSGQLIIGFALETENELNHAQDKLTRKNFDYIVLNSLNDPGAGFAHDTNKITVIDKDKNVQQFDLKSKEEVAQDILNIVLGKWSEA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The CTP binding site should be stable and able to withstand changes in environmental conditions.2. The presence of Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains is critical for the interaction of the designed protein with its intended targets.3. The designed protein should be optimized for phosphopantothenoylcysteine decarboxylase activity, metal ion binding, FMN binding, phosphopantothenate--cysteine ligase activity activity in order to improve pantothenate catabolic process, coenzyme A biosynthetic process.4. We require a protein that can specifically bind to the metabolites produced in the CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis, Cofactor biosynthesis pathway.5. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.6. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A7. We need a protein that can perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2 with high efficiency. The designed protein sequence is ",
    "response#1": "KHVLLGVCGGIAAYKSAELIRRLQDAGAAVRVIMTAGAQEFITPLTLQALSGNPVHTQLLDPEAEAGMGHIELARWADILLIAPATAEVIAKLAHGNGSDLLTTVALACQAPLAIAPAMNQAMWGHGATQENLATLKARGVHIFGPASGTQACGDVGLGRMSEPDALAETVVLGVSGGIAAYRSCELCRLLIKDGARVAVVMTEAAARLVGPATFQALSGRPVEVGWGGAASAAGMEHIDLGRDADAIVVAPATANTLAKIRLGLADNLLTSTVLASRCPLVLAPAMNTRMWDNPATRENLAWLRAQPRIRVVGPAEGSLACGEEGAGRMADPADILEAVRRVLDGPAPDLAGRRLVVTAGGTREPIDPVRFLGNRSSGRQGLAIASAAAHAGGSVQVIAANIESALLSALPAGVQVTRVGSALQMRDATIAAVARADALVMTAAVADFRPEVASVSKMKKDPTNEDVPTIRLIRNPDILAEIGHSEQRPPLVVGFAAETGTDEEILAYGADKAARKGADFICLNRVGESVGFGDVPNDIRVLSRAGAEEEVPLAAKGELARVVWDRVATLLGPA",
    "response#2": "KRILLVISGGIAAYKTLDLIRRLRERGAVVRCILTEAASHFVTPLSVAALSENRVYQELFSLTDEAEMGHIRLSREADLVVVAPATANILAKMAAGIADDLATTLLLATNTPILVAPAMNVVMWEHPATQRNLTVLVERGVHVVGPATGELACGETGAGRMEEPEAILAAILAEAAASALGPSELAGARVLVTAGPTREAIDPVRFLSNRSSGKMGYALAAAASRRGAKVTLVSGPTALAAAPGVELVPATSAAEMARAVRSRFSRCDALLMAAAVADFTVQRPARSKIKKDRIRGPLRLALVPTEDILASLGRSRRGRRPWLIGFAAETGDPVPAAKDKLARKRLDMVVANDVSEKGAGFEVDSNHLYLVDAGGVETLESDSKHALADAVWDLVAARLG",
    "response#3": "TVVLGVSGGIAAYRSCELCRLLIKDGARVAVVMTEAAARLVGPATFQALSGRPVEVGWGGAASAAGMEHIDLGRDADAIVVAPATANTLAKIRLGLADNLLTSTVLASRCPLVLAPAMNTRMWDNPATRENLAWLRAQPRIRVVGPAEGSLACGEEGAGRMADPADILEAVRAVLARGGAAALAGRRALVTSGPTFEPIDPVRFLGNRSSGRQGHAIAAALAALGADTVLVSGPTSLPDPPGVTVRRVSTALEMLAACLAELPVDAAVCAAAVADWRVDASLERKLKKAAGRPPTLHLVENPDILAAIAGAERRPLLVVGFAAETEDVVAHAVAKRQAKGCDWVIANDVAPGTAVFGGDSNTVHLLEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLQRVLLEEGASSPEVVLEGPKETLWTAMRAFRAERS"
  },
  {
    "reference": "MSDIDEKNDTKESSFSSDVVLLSLLISGSTLGAIAGYNRYLKQVTKATDIPNYMFRKRWMYGKVTAVGDGDNFHLFHTPGGIFGGWGWLRKVPKLPKSDSNGLIVSRKKTSNFYSGLKNSYHKFTGSYRYSSEYFLDLKVPYKNLRNLPTVPIRLCAIDAPERAHFGNTSQPYGDEALIWLRNRLLGKYVWVKPLSVDQYNRCVSKVVCWNWLGWQNISLQMVRQGLAVVYEGKTSAEFDREEFLYRFYERRSKAKKRGLWRQRVIETPGEYKKKIKK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein localizes to the membrane. The designed protein sequence is ",
    "response#1": "SVHKLRPGDIDVIAAMGDSLTAGAGIFADNEFNPNLIGFALGDSLTTHKESQLNIAESGAESADMMYMAEMLVKKIKNDPRINVQKHWKLISLMIGANDFCNEICSVSSPWSILENHKVDLLQVLRILRDNLPRTFVALIPPPHLKSLVDIRKEKSSFKCFVTTDVECSCLFGLAFQRYISIYYDIMRQWQILDMEIADYPEFQRNDFVVVAQPTLMNISIPLDSDGYSDMTYLSSDCFHVSQKTNARFANGLWNNLLETVMG",
    "response#2": "SVHKLRPGDIDVIAAMGDSLTAGAGIFADNEFNPNLIGFALGDSLTTHKESQLNIAESGAESADMMYMAEMLVKKIKNDPRINVQKHWKLISLMIGANDFCNEICSVSSPWSILENHKVDLLQVLRILRDNLPRTFVALIPPPHLKSLVDIRKEKSSFKCFVTTDVECSCLFGLAFQRYISIYYDIMRQWQILDMEIADYPEFQRNDFVVVAQPTLMNISIPLDSDGYSDMTYLSSDCFHVSQKTNARFANGLWNNLLETVC",
    "response#3": "SVHKLRPGDIDVIAAMGDSLTAGAGIFADNEFNPNLIGFALGDSLTTHKESQLNIAESGAESADMMYMAEMLVKKIKNDPRINVQKHWKLISLMIGANDFCNEICSVSSPWSILENHKVDLLQVLRILRDNLPRTFVALIPPPHLKSLVDIRKEKSSFKCFVTTDVECSCLFGLAFQRYISIYYDIMRQWQILDMEIADYPEFQRNDFVVVAQPTLMNISIPLDSDGYSDMTYLSSDCFHVSQKTNARFANGLWNNLLETVR"
  },
  {
    "reference": "GSIPCGESCVYIPCISSLLGCSCKSKVCYKD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Binds to and induces leakage in phospholipd membranes, particularly ones containing 1-palmitoyl-2-oleophosphatidylethanolamine (POPE) .. The designed protein sequence is ",
    "response#1": "DRPFYISLVRGQPDKVIAHTSKDQSLLNLERGIASLLQLRLDASQEEELDVSGLCRVSYNVKSSTKVEKTKRDCSLWDLRVNYNPEEALGVTQQAQETVFYELSSEGTLLHAESQENHRLNLAAKPDVGSFVKSSLILQHVSQGSEEVKQLQLGSLDKAIQSLLEWYRVFELESDVDGMISAIKEQTLEDQLKASLTELQSADVGKSSLALAYVKLIPLARITRQEQFEDLLTEHAEVLPQLVDLLGAVQTFDAHNATFGFLYKESETTSEQLDLLEKYLQSLAVATHPDRKIVEHLFGLLEQESIKKHLKLRESVIQTVATLTRQSGLDVEDPLLKEVRSYLLQGLTSKEPTLYIRALQNLQDPATIEALLEHAQTGEAPNLSVAALQALKAFPLGSFNSSHRLQFESIFYQRKRRFDSSARTLALDIILSLRPTQEQX",
    "response#2": "SVHSLKPADVKIIAALGDSLTAGTGIASDNLLDLDTEYRGLSWSIGGDASLEDVTTLPNIFQEFNVTIVGYSTGTGSENDSNAFLNQAVPGAQAEHLPAQARNLVRLMKTDPRIDFSADWKLITVHIGGNDLCNCCKDPDHYSAVNFISRIRETLDILHEQASAVPKALVSLVEVMDLLPLRQLFMDTQVQCPTYMADYLCSCVLTGEENSPNLTMVREVTRDYQLGIRRLVESGRYDTHENFTVIIQPFLQNPKIPLGQDGHPDVSYFSPDCFHPSQKGHSQLAKALWNAVLQPVGRC",
    "response#3": "SVHNLKPADIRVIAALGDSLTAGNGAASKPHDVADVLTQYRGVSWSVGGNENISTVTTLANILREFNPSLIGYSIGTGRETTDNAALNQAVAGNHAEHVPAQVRRLVDRMKNDTRINMQTDWKLITLLIGGNDLCDFCENPVHYSPENYTFNLQKALDMLHREVPRAFVNLVTILSITSLRELHKSANSCPKLLMRILCPCVLNPDDNSDEFKKLVYFNRRYQERTRQLVESGRYDTTDDFTVVMQPFLTNVNIPKTQEGLPDSSYFAPDCFHFSQKSHSQAARALWNNMLEPVGV"
  },
  {
    "reference": "MRSDKTKKGLERAPHRSLMRATGMSSEDIKKPFIAVCNAFNEVIPGHAHLDQVGKIIKDAVREAGGTPIEFNMIGVCDGIAMGHSGMKYSLPSRELIADSVETMVGAHAFDAMICIPNCDKIVPGMIMGAMRVNIPTIFASGGPMKAGKTKDGKVVDLISVFEGVAAHKQGKISDEELEELECKGCPGQGSCSGMFTANSMNCLCEAIGLSLPGNGTILALDPARHQYWKDAAKRAVEMAFADGPLPRDIVTQESLDNAFALDMAMGGSTNTVLHTLAIAKEAGVDYNLERINAVSKKCPNICKVSPSSHYHIEDVHAAGGISAILGEIAKKDGLLNLDCPTVSGKTLGETVVGCDSQDLECIRVVENAYSETGGLSILWGNLAEGGCVVKKAGVDPKMLVHTGPAVIFESQEEACEGILDGKVKAGDVVVIRYEGPKGGPGMQEMLAPTSYIMGQGLGDSVALITDGRFSGGTHGACIGHISPEAAEGGPIGLIKAGDIIEIDIPNNKLAVQLSDEELAERKKDWKEPAPRFTTGWLSRYAKMATNASNGAVLEG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit dihydroxy-acid dehydratase activity.2. For general function, the protein need meet that Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo- 3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3- dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively. The designed protein sequence is ",
    "response#1": "DVIHSLDAPYDAQGGLSILFGNIAPKGAVIKVGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAPTSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTNRTLKVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGHFVAY",
    "response#2": "DVIMPIERALYPQGHLAILKGNLSPEGCVAKITGLKNPVITGPARVFDSEDDAMAAIMDRRIRDGDVVVIRYEGPKGGPGMREMLAPTSALVGQGLGETVGLITDGRFSGGTWGMVVGHVAPEEFVGGPIALIREGDSVTIDAHQLLLLLLNISDEEMAARRKAWAQPKPRYVRGVLAKFGKLACTASRGG",
    "response#3": "RPLIGIASSYSEIIPGHMNLDKVVQAVKDGVRMAGGVPVTFGTIGVCDGISMNHKGMSYSLPSRQLIADSVEIVASAHAFDGIVLVPNCDKVVPGMMMAAARLDIPAIVISGGPMLAGNTCGKATDLSGVFEAVGAVKAGNMTEEELSELENTACPTCGSCSGMYTANSMNCLSEVLGLALPYNGTIPAVYSERLRLAKTSGMKIVDLVKKGLTPSKILTEEAFMNAITADMALGCSTNSLLHLPAIANEVGITDVIMPIERALYPQGHLAILKGNLSPEGCVAKITGLKNPVITGPARVFDSEDDAMAAIMDRRIRDGDVVVIRYEGPKGGPGMREMLAPTSALVGQGLGETVGLITDGRFSGGTWGMVVGHVAPEEFVGGPIALIREGDSVTIDAHQLLLLLLNISDEEMAARRKAWAQPKPRYVRGVLAKFGKLACTASRGAVIE"
  },
  {
    "reference": "MAILVSLLFLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGPLILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITRVAFGKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELDAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMFSGGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKETLRLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFVNNSVDFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLDMSEAVGITVHMKFPLQLVAKRHLS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The protein should have monooxygenase activity, oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen and be active in endoplasmic reticulum membrane.3. The protein must exhibit the following characteristics: Involved in the metabolism of compounds associated with the development of flavor in the ripening fruit process, possibly by acting as trans-cinnamic acid 4-hydrolase..4. The protein should have a high affinity for heme. The designed protein sequence is ",
    "response#1": "GCKAGFAGDDAPRAVFPSIVGRPRHTG",
    "response#2": "LRMVQ",
    "response#3": "GCCGCC"
  },
  {
    "reference": "MAKRVKVVLKEDILSLGKDGDVVEVAPGYARNFLLSQQKALAVTPSVLKQVEYRLAKKAELEAAKKQEAIDFETALKTIGRFSIKKQTGEDGVLFGTVTNGDVSEAIQLATQKEIDRRNIIVPEIHETGKYKVQVKLHSEVTAEINLEVIGN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein can be targeted to ribosome and perform rRNA binding. The designed protein sequence is ",
    "response#1": "MLS",
    "response#2": "MSKSTPAAVLLKKAAGLKSGSPRPNTQKVGTVTRAQLEEIVVAKQADLTASNMDAAVRTIAGSARAMGLEV",
    "response#3": "MRYRL"
  },
  {
    "reference": "MITTDRMAMVDRNAAALGVPRKQLMESSGNAVARAVRERAEEGDRVTLVCGRGNNGGDAMVAARFLEGFDLRVVLLGRPETISTQITRENWAALGAAEVPTETVTDSRSFDLDDPDVVVDAMLGTGVTGALREPEATVAEALNAADATVVAVDVPSGVDADTGAAEGAAVDADAVVTFHDTKPGFDDFDAEVVVEDIGIPDAAETFTGPGDLLSIRRSPGATKGDAGRAFVIGGGPYTGAPALASQAALRAGVDLSFVAVPESVKTPIQSYAEDLIVQPYEGDHLAPDHVDDLVDTAESHDDVVVIGPGLGSHDETVEAAERFLSEFEGRAVVDADALPVIPEVDTDATLVLTPNRKELVELGGPDVDDLRANQAAIADLSAELGHVVLAKAKDDVITDGERTRVCRAGTPGMTVGGTGDTLAGITAAMLATHDPFEAACMAPQINGSAAEAIAGEVGDGLLASDLLDAIPRAVWGDESDE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. We need a protein that can perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate with high efficiency.2. The protein contains novel YjeF N-terminal domains that confer a unique function or activity.3. The designed protein should be optimized for ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, ATP binding activity in order to improve nicotinamide nucleotide metabolic process, phosphorylation.4. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..5. The AMP, K(+), (6S)-NADPHX binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MMVTDLNCEYLGLSRLCLMESAGKSLAEEIGKIAVYTFSKPVKIIIFAGSGGNAGDAFVATRYLLNKGYDVDIYMLTQENKIKSNDAKSNFKILENLKPRLSHLKIYNLATVEDINSCELVNSDEFSDFIIIDAILGTGIKGKLRDKVRRAIEVINESKGLKISVDIPSGMNALTGKIEDLAVKPDYTISFHKIKTGVRISDEEKVGGLVTADIGIPFEAELALGGSPGFTGAITLTARAALRAGAGLVTAAVPRGCQPVVAAGAPEIMTWGLPETAGGSLGRSALDPLLERLENFSVLAIGPGLGRDPETVELVKSLLPRASIPLVVDADALNALATDTGILSQPHGPMVLTPHPGEMGRLIGCPAAKIQEDRLNIARRYAQEWRCVLLLKGARTVVACPDGRAYINPTGNPGMATGGSGDVLTGIIAGLIAQGMSLDKAAAIGAYLHGAAGDLAAEELGQEALIAGDILD",
    "response#2": "MKLLDTAAGRFGVSEKELMRSAGEAIYREVIAAVPDGDIAVVCGSGNNGGDGYAAAAMLAEGGRSVTVIASAPPRGESSRFFAERYASMGGETLHYADSPMQCADVIADSAVVIDALFGFSFRGQLSGDYAELTELINGSDAFVVSADMPSGVYADGGYADGCVSADVTCTFSVNKPATVSYPAYGFCGRVVLCDIGVPEEAMEPPDVGARWPVPAATDDKYTQGVVGVLAGSAQYPGAAILCSGAAVAATSGMVRYVGSAAADVVTHYPEVVAATGMKEAGRVQSWVVGPGFGTDTSSRDVLSQVLTSDLPVVVDADALTILSSSPELVRGRTAPTLLTPHAGEFERLTGAAPGSDRVSAVRELASTWGVSVLLKGRATVIAGPDGAVVVSDAGGSAASTAGSGDVLAGILGALLASGLDTVTAAACAARVHALAAATAAHGRGGEFAAPISASPLLGAVSDAIRTGLLAGSAQFRGAAVLAAGGAAEGGAGMVRFVGPDEVATQVLNRWPEVVTSADVADTGRVQAWAVGSGLGDGRAAEVAAVLDTGLPVLVDADGLRHLPETLDGRALLTPHAGELARMMDADRGDVEAHRLRYVQAAARRWNATVLLKGSTTLVAAPDGRIRVNPTGTPALATAGSGDVLSGLAGALLATGMEPFDAGSAAAYIHGLAGQAAAEGSGL",
    "response#3": "MRAMDAQTCKLHAMTGLALMERAGRAIFSKLSTTKPWCETRPLIVCGPGNNGGDGLVVAEALLLAGMDVSVCLVSEDQHLSTENRQVYERLKDRVHRIQRSEDIEWLKQQISCHDWILEALFGIGLTRPIEGVFKAVVALLNASGKSIVSIDLPAGLHTDSGQVLGMAVNATETWAIQAFKFGHFLADGKDYCGRLDCVDVGIVPVETEPPDVGARWPVPAATDDKYTQGVVGVLAGSAQYPGAAILCSGAAVAATSGMVRYVGSAAADVVTHYPEVVAATGMKEAGRVQSWVVGPGFGTDTSSRDVLSQVLTSDLPVVVDADALTILSSSPELVRGRTAPTLLTPHAGEFERLTGAAPGSDRVSAVRELASTWGVSVLLKGRATVIAGPDGAVVVSDAGGSAASTAGSGDVLAGILGALLASGLDTVTAAACAARVHALAAATAAHGRGGEFAAPISASPLLGAVSDAIRTVREPPDVGARWPVPAATDDKYTQGVVGVLAGSAQYPGAAILCSGAAVAATSGMVRYVGSAAADVVTHYPEVVAATGMKEAGRVQSWVVGPGFGTDTSSRDVLSQVLTSDLPVVVDADALTILSSSPELVRGRTAPTLLTPHAGEFERLTGAAPGSDRVSAVRELASTWGVSVLLKGRATVIAGPDGAVVVSDAGGSAASTAGSGDVLAGILGALLASGLDTVTAAACAARVHALAAATAAHGRGGEFAAPISASPLLGAVSDAIRTGT"
  },
  {
    "reference": "MESRPGSASSYHSHAAGNTQSRPGSATSVESSNRLSGKPLGAEKLMAACEKIYKSFNPAQVTLDTHVDNCIGQLSVHNSFDDSFIRQVVYGTVRYRRLLGALMDSFYHYNGGAASREDVDMYKLYSYLTIFRLEELNFSNFQRLVDAMTPQKMYVLLKYMFNPQYMREVVREDWLKLYDKEFVDEIIDRLLSWKSESKEMLGRLEYKVTLSRKEDDEKETLGTGYAAARSTTVPQPFNLTQPKPRPLPVEDPPPPPIRAKPAPRPREGPTKEEVALAAAREANRAAAERKAAKAAPFKLRVLERPTNIDKIREELEAERTRELTFKGIRAAPPPPVPNAQVRLNAAAILREDALYRRKQQEEADALKRYEAELRDASSFKAWQNAMLEQDEAARAASVERRRQEMAAAQENAIRARMAAQEANAELARAAKEEAKRIEEDLKRDREEQARLNALRRDAVVEARQNVQAAVEKMSQERRLAAEEERRKQQEDARARAEVAAREMAERRDIILQLKALEKVPKQRVKEFDPTETGPDHGLLETMSLVELRERLNVAKRRQREEEERQRAEILRQKQERESALLEKAANIQRVRRVAAAQAAQRRATSAETIQRKNTEVSKAREADVLQLADKLDAKRAALAAERARLAAEQKRTRFEQMQAAAGAAVVEETKFRELRAGAQREAKTRQENALASATVYEATKARQQNVRLKNVRQELKAKDDFMRAYDEKLAALRGQAGAESAADLARRTQMAQTQRAAEATVRNRTTTT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should exhibit Pro residues, Polar residues compositional bias.2. Include a signal peptide as an essential component of the protein.3. The designed protein should be expressed specifically in the motile cilium The designed protein sequence is ",
    "response#1": "QSCDVLRDENSKRILYRTIEERNAQIEHKMAQRIMEAEEKRMWHEMSEVERQKMEQRYLDDKRRDREKREEVLRILDEQVRQVNARRAEASMLRRAEIAELNATWRQMAADQEAADVQERENMKKLAAELQEFNRIKQMEISEAERSERELDLKILQEALSKEAADEAAELAFRERRREEMRRYREQLALMMEKEREETAERDALILKAQLEQEAKRDAELAARDEARRQLMAQVDAIRQIQIQEKLAKRLERAEEKAFERAQMAEEVAKAESDAAAKDAADRKAGIQRRLELQTMMVAKAHMKAAELDEKLAEGEATKRVEDQFKAKVNC",
    "response#2": "PGEPTLIPELSLAGNVTPNDQCPRVEVSRREDVRRRSVYLKVVFNSKEVSRTVSRPLGADFRVHFGQIFNLQIFNWPESLMLQVYETIGHSGTTLLAEVFLPIPETTLVTGRAPIEEVEFSC",
    "response#3": "CETVEATFHGVAYLLTVSTIEGETLCVEVEQKSDCSRWRGDFTSRYIEDITAKTGNFKKFPVFVKMLLSALKQASDSVFVDLLTYQDLEVLKSRKAGGQAPPPRTQPPNNKRYLIMTYAAEFDRVHYPLPLLFC"
  },
  {
    "reference": "MRVKGIQMSWQHLWRWGTLILGLVIICSASNLWVTVYYGVPAWEDADTILFCASDAKAYSEEKHNVWATHACVPIDPDPQEIPLENVTENFNMWKNPMVEQMHEDIISLWDESLKPCVKLTPLCVTLKCTSLNNTRGNTTQENSNSTMNEGGKLMTNCSFNITTEIRDRQKAEYALFYKHDVIQVNDNSSTYRLINCNVSTIKQACPKVSFEPIPIHYCAPAGFAILKCRDKGFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEGNVTIRSENITNNVKTIIVQLDEPVQINCTRPSNNTRRSISFGPGQTFYATGDIIGDIRQAHCNVSRRSWRDTLNKVAKQLGKHFNNKTINFNKPSGGDLEITTHSFNCRGEFFYCNTSGLFNNSMRNDSTNGTITLPCKIRQIVRMWQSVGQAMYAPPIAGNITCISNITGLLLTRDGGDNSTNNEIFRPGGGDIRDNWRSELYKYKVVKIKPLGIAPTRARRRVVGREKRAVGLGAVLLGFLGTAGSTMGAASITLTVQVRQLLSGIVHQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLALERYLRDQQLLGIWGCSGKLICPTNVPWNSSWSNKTFEQIWENMTWIEWEREINNYTQQIYSLLEESQNQQEKNEQDLLALDKWESLWSWFSISSWLWYIRIFIMIVGGLIGLRIVFAVLSIVKRVRQGYSPLSFQTLIPNPAEVDRPGETEEGGGEQGRTRSTRLVNGFLALVWDDLRSLCLFSYHRLRDFVLIVT",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.2. The protein design should include a Di-leucine internalization motif motif to ensure proper function.3. A protein that contributes to positive regulation of plasma membrane raft polarization, fusion of virus membrane with host plasma membrane, positive regulation of receptor clustering by functioning in plasma membrane, host cell endosome membrane, host cell plasma membrane, virion membrane with structural molecule activity.4. For general function, the protein need meet that The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane The designed protein sequence is ",
    "response#1": "MRVKGIRKNYQHLWRWGTMLLGMLMICSATEQMWVTVYYGVPVWKEAKTTLFCASDAKAYSKEVHNVWATHACVPTDPNPQEMVLGNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCSNVTRHGNNSKSNVTNEEIGMKNCSFNITTEVRDKKKQVYALFYKLDVVQMDTNTSYRLINCNTSAITQACPKINFEPIPIHYCAPAGFAILKCNDKEFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIIIRSENITDNTKNIIVQLNEPVTIKCIRPYNNTRQGIHIGPGQALYTTDIIGDIRRAHCEINKTEWNTTLQQVAKKLGDLLNQTAIFQPSSGGDLEITTHSFNCGGEFFYCDTSELFNKTSNSTGGPRDTITLPCRIKQIINMWQGVGKAMYAPPIEGLIKCSSNITGLLLTRDGGTNNSKEETFRPGGGDMRDNWRSELYKYKVVRVEPLGLAPTRAKRRVVEREKRAVGIGALFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTTVPWNSSWSNKSLSEIWDNMTWMQWDKEVSNYTNIIYDLIEKSQNQQEMNEQDLLALDKWAGLWXWFDISNWLWYIKIFIXIVGGLIGLRIVFALLHIINRARQGYSPLSLQTLLPATRGPDRPEGIDEEGGERDRDRSGRLVNGFL",
    "response#2": "MRVMGIQMNWLNLWKWGTLILGLVIICSASDKLWVTVYYGVPVWRDAETTLFCASDAKXXEKEVHNVWATHACVPTDPDPQEIXMKNVTENFNMWKNNMVEQMQEDIISLWDQSLKPCVKLTPLCVTLICKELNSTSSNITSXDXTSYNXSMATEMKNCSFNMTTELRDKKKTVYSLFYKLDIVSTEGTNDSYRLINCNTSTMTQACPKVSFEPIPIHYCTPAGFAILKCRDKTFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVMIRSENITDNSKIIIVQLTXPVNITCIRPGNNTRTSIXIGPGQAFYTTGDVIGNIRKAYCHVNGTAWNDTLQKISXXLGXHFXNKTIIFRNSSGGDLEVTTHSFNCGGEFFYCNTTVLFDSIWDKNSTEDKNGTGTNSTXDIILPCKIKQIINMWQRVGQAMYAPPIRGVIRCESNITGLLLTRDGGEEISNVTFRPTGGNMRDNWRSELYKYKVVKIEPIGVAPTRARRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASXTLTVQARQLLSGIVQQQSNLLRAIEAQQQLLRLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNYSWSSKNQSEIWDNMTWMQWDREVINYTNIIYDLLEQSQNQQEKNEQDLLALDKWAXLWNWFDISNWLWYIRIFIMIVGGLIGLRIVFTVLSIINRVRQGYSPLS",
    "response#3": "MRVMGIQRNCQNLWRWGTMLLGMLMICSATEKMWVTVYYGVPVWKEAKTTLFCASDAKAYSKEVHNVWATHACVPTDPNPQEMVLGNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCSNVTRHGN"
  },
  {
    "reference": "MNCEHFGICGGCTNIQDYSTQLQAKHNLTLQEFQSFLDTKSKNFQYPTNPLAIEVFASPQEGFRARAEFRFSHVFQNKSGLDFAMNAFGYNHRVPIKKCPILLPTLQDIMPLLLHYLNTYDLLNHKLYACNLLSSLQNEIIITLIYHKSLDSHWESLALKIQKELEYTLNTNIHIIGRSKNHKHILSNDIICEHLTLFANTPKERTYTFFKQESRFCQPNPFINTQMLEFIVSALSSIYTPQTPCDMLELYCGSGNFTIPLASIFRHIFATEVVKSAITLLQKNMAKNNIENIIPARLNAFESIQALRKERVFFRLKNIDLDAFAFDCVLIDPPRSGVGEEEVLYFLQNFNTIIYVSCNPHTLLNDLRILSQSHYVMRFGLFDQFPHTYHRECIVILRKSNKIL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Dual-specificity methyltransferase that catalyzes the formation of 5-methyluridine at position 54 (m5U54) in all tRNAs, and that of position 341 (m5U341) in tmRNA (transfer-mRNA)..2. The designed protein should have a high specificity for S-adenosyl-L-methionine binding, with minimal off-target effects.3. The protein with the reaction S-adenosyl-L-methionine + uridine(341) in tmRNA = 5-   methyluridine(341) in tmRNA + H(+) + S-adenosyl-L-homocysteine, the Proton acceptor must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MLKKLHVVLYQPEIPANTGNIARTCAATNTTLHLIRPLGFSTDDKMLKRAGLDYWEFVNVVYHDSLEELFEAYKKGKFFFITKFGQQPHTSFDYTDLDEDYFFVFGRETSGLPKDLIQNNMDRCLRLPMTEHVRSLNLSNTAAILVLEKG",
    "response#2": "MLHVVLYQPEIPANTGNIARTCAATNTTLHLIRPLGFSTDDKMLKRAGLDYWEFVNVVYHDSLEELFEAYKKGKFFFITKFGQQPHTSFDYTDLDEDYFFVFGRETSGLPKDLIQNNMDRCLRLPMTEHVRSLNLSNTAAILVELE",
    "response#3": "MTLHVVLYQPEIPANTGNIARTCAATNTTLHLIRPLGFSTDDKMLKRAGLDYWEFVNVVYHDSLEELFEAYKKGKFFFITKFGQQPHTSFDYTDLDEDYFFVFGRETSGLPKDLIQNNMDRCLRLPMTEHVRSLNLSNTAAILVVE"
  },
  {
    "reference": "MGFDYALVHLKYTIPPAVLLTWLYRPFFTKLDVYKVGYLVFVAVVSTIPWDSYLIRTGIWSYPSHVIIGPKLYDIPLEEVFFFIIQTYNTSLLYLLLSRPTFQPVYLKTERGTAQRQWRYMRLAGQVFFLALIAWGWRCIRRGGLGTYTGLILVWAGPFLLMLWTLAYQFILALPLTNTALPIFLPTLYLWVVDTLALRRGTWVISTGTKYGLNLWDGLEIEEAVFFLATNVLIVFGQLAFDNALAVLYTFPHLFTDPSLLPSPVLLMRALLTPCSKYDDLRIKGLDEAVHRLKRKSRSFYLASATFPGPLRADLLLLYSFCRVADDLVDNASDADEARAWIAKMRNFLNNVYNDKLPQSAVHTQIYDDFPPSTQSAFLQLPATKLSPQPLEDLLHGFEMDLAFQQGPIISTAENLRVYSERVAGTVAQMCIQLIFYWYPSTLAIGEKNAIVAAGNSMGVALQYVNIARDIEVDAQIGRVYLPLNWLFEAGLSYDDVLKKPNQAQIQTLRKHLLNDAFSVYEKAKDSIERLPIEARGPIRVAVESYMEIGRILRNEQYQVKAGRATVPKSRRIVVAWRTLNLPDNYTDETTFLDHLQRNPRLQPYEFWSLMSDATVIVQHLASVIIFCCCFVAIIQNRVSPIAVVGWASICTVLAWLLWDHWMGQEFDIIANVPTSNTEESVPNAPQAYSLRTQQRIATAKSAVLIYAALLGLSPILKSLTRSTTSDSIWALSTWLLMMNVAFFDYTGGTGAQYE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. We require a protein that can specifically bind to the metabolites produced in the Glycolipid biosynthesis pathway.2. The designed protein should have 15-cis-phytoene synthase activity, lycopene beta cyclase activity, geranylgeranyl-diphosphate geranylgeranyltransferase activity, farnesyltranstransferase activity within membrane to regulate carotenoid biosynthetic process, GPI anchor biosynthetic process.3. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "HNHLLLKFAKLDFNMLQKLHQRELSDLTRWWKDLDFANKYPYARDRLVECYFWILGVYFEPKYSRARKMMTKVLKMTSIIDDTFDAYATFDELEPFNDAIQRWDANAIDSIQPYMRPAYQAFLDIYSEMEQVLSKEGKLDRVYYAKNEMKKLVRAYFKETQWLNDCDHIPKYEEHMENSLVSGGYMMIPTTCLVGMEEFISIETFEWLMNDPLIVRASSLIARAMNDIVGHEVEQERGHVASLIECYMKDYGASKQEAYAKFKKDVTNAWKDINKEFFRPTEVPMFVLERVLNLTRAADTLYKEKDAYTNAKGKLKNMINSILIESVV",
    "response#2": "HNHLLLKFAKLDFNMLQKLHQRELSDLTRWWKDLDFANKYPYARDRLVECYFWILGVYFEPKYSRARKMMTKVLKMTSIIDDTFDAYATFDELEPFNDAIQRWDANAIDSIQPYMRPAYQAFLDIYSEMEQVLSKEGKLDRVYYAKNEMKKLVRAYFKETQWLNDCDHIPKYEEHMENSLVSGGYMMIPTTCLVGMEEFISIETFEWLMNDPLIVRASSLIARAMNDIVGHEVEQERGHVASLIECYMKDYGASKQEAYAKFKKDVTNAWKDINKEFFRPTEVPMFVLERVLNLTRAADTLYKEKDAYTNAKGKLKNMINSILIESVL",
    "response#3": "HNHLLLKFAKLDFNMLQKLHQRELSDLTRWWKDLDFANKYPYARDRLVECYFWILGVYFEPKYSRARKMMTKVLKMTSIIDDTFDAYATFDELEPFNDAIQRWDANAIDSIQPYMRPAYQAFLDIYSEMEQVLSKEGKLDRVYYAKNEMKKLVRAYFKETQWLNDCDHIPKYEEHMENSLVSGGYMMIPTTCLVGMEEFISIETFEWLMNDPLIVRASSLIARAMNDIVGHEVEQERGHVASLIECYMKDYGASKQEAYAKFKKDVTNAWKDINKEFFRPTEVPMFVLERVLNLTRAADTLYKEKDAYTNAKGKLKNMINSILIESVS"
  },
  {
    "reference": "MEKKIALIAHDKKKEDLVNFVKQNYLFLSKFKLIATGTTGSKIQQATDLTIFKYKSGPMGGDQQIGAEVAEGNILAIFFFRDPLTSQPHEPDVSALIRLCDVHKIPLATNVKTAEILIKGLESLIF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be able to bind substrate ligand in a variety of conditions2. The designed protein have MGS-like domains with specific sequence motifs that are crucial for its activity.3. The designed protein must possess methylglyoxal synthase activity to support methylglyoxal biosynthetic process under certain conditions.4. For general function, the protein need meet that Catalyzes the formation of methylglyoxal from dihydroxyacetone phosphate. The designed protein sequence is ",
    "response#1": "MEGQRCIALIAHDEKKDDMADFARHHQKVLASFRIVATGTTGGRVQEACPGLEVIRLKSGPLGGDQQIGAMIATGEVDMLIFFTDPLTAMPHDVDVKALTRLATVYDIPMALNRATAENLIDFNSAY",
    "response#2": "MSKVKNIAVVAHDNCKKELLDFVDCNHNILSRHNLVATGTTGGLVEKMIMERVEQKADEGYEFKPVNRMKSGPLGGDQQLGAMISEGKIDVLIFFWDPMQPQPHDVDVKALLRLAVLYNIPTASNRSTAEFLISSPFFEGEFQRKETDFSSYTQRKA",
    "response#3": "MEGQRCIALIAHDEKKDDMADFARHHQKVLASFRIVATGTTGGRVQEACPGLEVIRLKSGPLGGDQQIGAMIATGEVDMLIFFTDPLTAMPHDVDVKALTRLATVYDIPMALNRATAENLIDFNSAI"
  },
  {
    "reference": "MVRRECLEFREQGIISAARMRAVDENAMALGVTSLQLMESAGKALSDLVRSLDADTVLILCGKGNNGGDGMVAARHLQDLEVSVIYLEGQGRSPDCDHQLKVLSSCPVDLAPARCHEDVVALNTHFQEAGCIVDALLGTGSTGTPREPIRSLVEASQRTTAVKVSADVPTPGFVPDHILAFHRPKVEGSLVADIGIPLEAEITVGPGDLTVMQRKDPGAHKGAGGEVLVVGGGPYQGAPYLAGLGALRAGADIVRIASPVFEPVPDLIYERLEGKRIGARHVETLKALAERADVVVMGNGLGDKSHGVMVELARSCRRAVVDADAIRTPLPQAEDTIYTPHAGEFARITGALPPGEPVPRARAVRDAARTGTILLKGKTDIISDGKRVRFNRTGSPLMTVGGTGDVLAGVTAALFCHLPAFESACLAAYVNGRAGMGIEKAFGGGMLPTDLVDRIPLELFQRSEPT",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be designed to have high stability and activity towards (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.2. The presence of Mg(2+) is necessary for the protein to function in the desired environment.3. The designed protein should have ATP binding, NADPHX epimerase activity, NADHX epimerase activity, kinase activity to facilitate phosphorylation, nicotinamide nucleotide metabolic process.4. The designed protein must have at least one YjeF N-terminal domain. The designed protein sequence is ",
    "response#1": "TTSQIRRLEADWIEQCGSNWSQVLMEVAGRGAAKIALKMWENDPGNVIVICGRGNNGGDGMVIARYLHMWGLPVSVWLVSKDSSPKIEMSTVESKTNRTILENLGVDVTVSNESPLHALSEVTLIVDALFGTGLDRDLEGAFRTAVDAINRSGKSVLAVDMPSGVNSDTGKVMGSAVRADHTVTFGYLKPGLLCHPGANLAGDLSVIDIGLAYGHKGTFGDVLFIGGASSYYGAPVFSSLSFLKAGGGYARLLAPKSMVPFMSAKANEVVYLPVEETERGFISYKALSSILEMASKCHMVVIGPGLGISEDGRRLLFDLLKRVDLPVLIDGDGLTLLSQDMEMLKARKGPTILTPHPGEMQRISGTNIQEIGQDPISFLKDFSKAHQVYLVYKVARSIVVTPEGKVYINVTGNSGMATAGSGDVLTGCIASMYCHSKDVGFALMAGTFIHGLSGDMAALAKGEDGITANDIMESLDRKDIVNALPKHRPTDEKYTRGVVGIATGSKTYPGAAILSTWGALAGPTGMVRYAGGARQYVVQAHPSVVATDRITEAGRVQAWVVGSGLGTDEAAATAVRTVLASSLPVVLDADALNLLVDGTMADLMRGRSAPLVLTPHDREFARLAGEEPGPDRVAAAQRLAAHTNAVVLLKGDRTIVATPSGQAYANPTGSAALATGGTGDVLAGLLGSLLAGGLRPEVAAVTAAYLHGLAGREAARQGPVSASDVAAALRPVVNDLRR",
    "response#2": "PAAEKYTRGVVGVATGSATYPGAAVLSVGGAVAGPTGLVRYAGSAAAQVLDRYPSVIATGRVADAGRVQAWVCGSGLGGGEHAVTELRSVLAAPVPVVLDADALTMLVDGSWAEQLRGRQAPTVITPHDREFARLAGQPPGDDRVGAALRLAAWMRATVLLKGDRTVVAGPDGRAYVNATGTPALATGGTGDVLAGLLGSLLAAGLDPQRAALAAAYVHGLAGRAAARSGPVTAVSAWWPSLAPAAEKYTRGVVGVATGSATYPGAAVLSVGGAVAGPTGLVRYAGSAAAQVLDRYPSVIATGRVADAGRVQAWVCGSGLGGGEHAVTELRSVLAAPVPVVLDADALTMLVDGSWAEQLRGRQAPTVITPHDREFARLAGQPPGDDRVGAALRLAAWMRATVLLKGDRTVVAGPDGRAYVNATGTPALATGGTGDVLAGLLGSLLAAGLDPQRAALAAAYVHGLAGRAAARSGPVTAVEVAAALRPA",
    "response#3": "MDPGPGLVVSGIPVADPAPGSVPAIAPAPGLVLVLAGPGNNGGDSLEAAAHLADAGLAVTVLHSPPAGTQSPERAAAWQRAASSGAHIIDVSSPAAQATGGRQQRQSSSAPLTATWSAGAQPLTGSQKAASSDKNTIEELAATALPSAHWQLVIDGLFGIGLARPVTGTLRALVAAVNQLNCPVLALDVPSGLDADTGAIVGPDGIALRASHTITFIGNKPGLHTNQGRDHAGLVEVAHLDIAPSLFRDSHKGANGHVLAIGGDHGTGGAVRMAAEAALRTGAGLVSVATREENVLAINAARPELMPRGANGPQALQPMLEKATVVALGPGLGQGAWGHALWTTALDAGLPTVLDADGLNLLQAEPRHLPAKTVLTPHPGEAGRLLGMATADVQADRFAAVRELAHRHHAVVVLKGNGSLIASPDGEVSVCPWGNPGMASGGMGDTLTGIVAGLLGQGCDPYRAACLGVGLHARAADVAARAGQRGLLAGDLLEPLR"
  },
  {
    "reference": "MGEEIKRTIKFTLVFMVLLGLVYPFVMTGIANLLFPYQAKGSIIKVDGKPVGSELIGQKFTDPRWFMGRPSAVDYDATSSGGTNYALSNPKFHEELEKNIEEFLKKNPGVKRSEIPADIVTSSGSGLDPHISPKAAYLQVKRVAKVNGLPEEVVKKLVDKNIEGRFLGLFGEPRVNVLKLNLSLLEEIKKHKK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The designed protein localizes to the plasma membrane.3. The protein must exhibit the following characteristics: Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm. The designed protein sequence is ",
    "response#1": "VLAMSGRAIEAAGDVDVLLLDKTGTITLGNRKASEFLPVDGVTEQELADAAQLSSIADETAEGRSIVVLAKERFDIRGRDFAEMHAEFVPFTATTRMSGIDYQENTIRKGAADAVRAYVTANGGTYPKECDAIVSKVAGAGGTPLVVVRNNKVLGVIYLKDIVKNGVKERFLDLRKMGIKTIMITGDNPMTAAAIAAEAGVDDFLAEATPEAKLELIREYQREGHLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEAGNMVDLDSSPTKLIDIVRIGKK",
    "response#2": "VLAMSGRAIEAAGDVDVLLLDKTGTITLGNRKASEFLPVDGVTEQELADAAQLSSIADETAEGRSIVVLAKERFDIRGRDFAEMHAEFVPFTATTRMSGIDYQENTIRKGAADAVRAYVTANGGTYPKECDAIVSKVAGAGGTPLVVVRNNKVLGVIYLKDIVKNGVKERFLDLRKMGIKTIMITGDNPMTAAAIAAEAGVDDFLAEATPEAKLELIREYQREGHLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEAGNMVDLDSSPTKLIDIVRIGKDA",
    "response#3": "VLAMSGRAIEAAGDVDVLLLDKTGTITLGNRKASEFLPVDGVTEQELADAAQLSSIADETAEGRSIVVLAKERFDIRGRDFAEMHAEFVPFTATTRMSGIDYQENTIRKGAADAVRAYVTANGGTYPKECDAIVSKVAGAGGTPLVVVRNNKVLGVIYLKDIVKNGVKERFLDLRKMGIKTIMITGDNPMTAAAIAAEAGVDDFLAEATPEAKLELIREYQREGHLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEAGNMVDLDSSPTKLIDIVRIGKG"
  },
  {
    "reference": "MSDLLNRRLSLLGANLPLLKQCLHGIERECLRVTDEGRLAQTPHPESLGSALTNEQITTDYSESLLEFITPALPDPAQVLESLEQTHRFVYSKLGDELLWSPSMPCTLPAEEDIPIAEYGASNIGKLKHVYRKGLALRYGRTMQCIAGIHYNFSLPEALWPLLRASDGNEQSDRDYQSAAYIALIRNFRRYSWLLMYLFGASPALDKGFLRGRPHQLEELDAETLYLPYATSLRMSDLGYQSNAQAGLTPCYNNLASYTDSLRKAVGTPYPPYVEIGTHVDGEWVQLNTNILQIENEYYSNIRPKRVTYTGERPIQALTSRGVQYVEVRCLDINPFLPVGIDLTEARFLDAFLLFCALEDSPLLDNGECGQCTDNFLTVVKEGRRPGLELRRDGHPIELKAWASDLLGRIGQLAELLDRAQGGDEHAKALAAQQAKVDDASLTPSAQVLARMGEHEESFIQFSLRQSRLHAETFREQPLPTERQQAYETLARNSLAEQSRLEQEEVGDFDLFVGAYQASILAISN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein should be able to manipulate the activity of enzymes in the Sulfur metabolism, glutathione biosynthesis, glutathione from L-cysteine and L-glutamate: step 1/2 pathway, either by activating or inhibiting their function.2. The protein should have a low Km value for ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-   cysteine + H(+) + phosphate.3. The designed protein must possess glutamate-cysteine ligase activity, ATP binding to support glutathione biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "ALLNADAAATLAGIRRGIEKESLRITTDGTLAQTPHPRALGSALTHPYITTDYSEALLEFITPPSTELHKPIEFLEQLHRYVYSHIGDEVLWVNSMPCMIGKDSDVPVAQYGSSNVGRMKTVYREGLGHRYGRKMQTIAGIHYNFSYPDAFWKLNQQLEGDNAPLQDYISRRYFDLTRNFQRYSWLLVYLFGASPALCASFLAGREHDLLERFDHSLYRPQATSLRMSDLGYQNNAQSSLAISYNNLDEYVSTLTHAINTPEPAYEKMGVKVTDADGNVKYQQLNANILQIENEYYSSIRPKRTIKPGERPTTALQERGVEYIEIRALDLNPFEPVGINQQEIRFLDLFATYCLLRESPQLEKCDLHASKENLRKLLQHANSDLPLLQANFGLERESLRINKTNHRLAQTPHPTALGSRQFHPYIQTDYSESQMELITPVAHSSKEVLRFLGAITDVAERSIDQNQYLWPLSMPPQITEDEIEIAQLEDDFEFSYRQYLDKKYGKILQSISGIHYNMELGADLMNELFELSGYQSFIDFKNDLYLKVAQNFLNYRWFLTYLYGASPLAEKGFLNEELSQTVRSIRNSHLGYVNTDDIKVPFDSLENYISSIEHYVKSGALSAEKEFYSAVRLRGSKHNRDYLTKGITYLEFRCFDLNPFNNRGITQETIDSVHLFILAMLWLDTPKKLNQALDKALIAEAAGR",
    "response#2": "LLQHANSDLPLLQANFGLERESLRINKTNHRLAQTPHPTALGSRQFHPYIQTDYSESQMELITPVAHSSKEVLRFLGAITDVAERSIDQNQYLWPLSMPPQITEDEIEIAQLEDDFEFSYRQYLDKKYGKILQSISGIHYNMELGADLMNELFELSGYQSFIDFKNDLYLKVAQNFLNYRWFLTYLYGASPLAEKGFLNEELSQTVRSIRNSHLGYVNTDDIKVPFDSLENYISSIEHYVKSGALSAEKEFYSAVRLRGSKHNRDYLTKGITYLEFRCFDLNPFNNRGITQETIDSVHLFILAMLWLDTPKKLNQALDKAL",
    "response#3": "KLSDPASRQALKSMQRGIEREALRIEKSGHLALDKHPKALGSALTHSRITTDYSESLLEFITPVFADIDELVEDLTLTHAYSVRHLNGQRLWPVSMPCYLGDGADIPIADYGSSNSGQMKSLYRKGLTYRYGAQMQIISGVHFNFSVSDKLWHRLYELSDKSLSLDDFISDSYFGLIRNYRRLVWVLPYLFGASPALCSTFIQDPKTSNFPFEVIGDGTLFMPYATSLRMSDLGYTNQEQDNLNISYNSLTDYLTGMQAAINMPSANFAKIGVKVDGEYRQLNDNILQIENEFYSPIRAKRVAKGSEKPSESLARAGVEYIEVRALDVNPYSAVGIEKSQIRFLDLFLLNCLLQPSAPSDATEEAEIAENLQAVLLTGHTSQADPAAVHAWLEKHPQALQGIQRGLERETLRVNADGTLATTGHPPALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDVHRYTARQLGDERMWPLSMPCYIAPGQDIELAQYGTSNVGRLKTLYREGLKNRYGALMQTISGVHYNFSLPMAFWQAKCGVEDAESGKEAISAGYFRSIRNYYRFGWVIPYLFGASPAICSSFLQGKPTTLPFEETGNGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLHEYVAGLKRAIKTPSEEYAKIGLQKDGKYLQINSNILQIENELYAPIRPKRVTRRGETPSDALLRGGIEYIEVRSLDINPFSPIGVDAQQVRFLDLFMVWCALADAPEMSSDELLCTRTNWNR"
  },
  {
    "reference": "MRVMETQMNWPHLWKWGTLIIGLVIICSASDNLWVTVYYGVPVWRDADTTLFCASDAKAQETEVHNVWATHACVPTDPNPQEIYLENVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTDDVKFVNLTNSNNSTNETSSYNIGNITGEVKNCSFNMTTEIIDKKQKVYALFYKLDIVPMSNSNSSDSSKYILINCNNSVIKQACPKISFDPIPIHYCTPAGYALLKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEKIVIRSENLTNNAKTIIVHLNKSVEINCTRPSNNIRTSMRIGPGRVFYKTGSIMGDIRKAYCEVNKTKWVEVLTQVNNTLKENFKNKTIIFQPPSGGDLEVTMHHFNCRGEFFYCNTTNLFNTTNLFDNSTKNDPIILPCRIKQIVNMWQGVGQAMYAPPINGIINCVSNITGIILTRDGGGNTTRGNETFRPGGGNMKDNWRSELYKYKVVQIEPLGVAPTRAKRRVVDREKRAVGIGAMIFGFLGAEGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKTICTTDVPWNSTWSNKSFEEIWNNMTWVEWEREISNYTNQIYTLLIESQNQQERNEKELLELDKWASLWNWFDITKWLWYIKIFIMIIGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTPFQQQREPDRPEGIGEEGGERGRDGSVRLVNGFLALVWDDLRSLCLF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The ideal protein would be one that plays a critical role in positive regulation of plasma membrane raft polarization, positive regulation of receptor clustering, positive regulation of establishment of T cell polarity, fusion of virus membrane with host plasma membrane, virion attachment to host cell through structural molecule activity within host cell plasma membrane, plasma membrane, viral envelope, host cell endosome membrane, virion membrane.2. For general function, the protein need meet that Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; Transmembrane protein gp41: Acts as a class I viral fusion protein; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain3. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "MWVTVYYGVPVWRDADTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVKQMHKDIISLWDQSLQPCVKLTPLCVTLNCNRVNNSSSSNSTNNXXTSSNSTVSPGIKXEMMNCSFNMTXXVXXKKXKVSALFYRLDVXQIDSEQTNSTXYRLINCNTSTVTQACPKVTFEPIPIHYCAPAGFAILKCKDKNFTGTGPCXNVSTVQCTHGIRPVXTTQLLLNGSLAEERVMIRSENLTNNTXTILVQFQNPVRINCTRPSNNTRKSLRIGPGQTFYATGEVIGDIRRAHCNVSTQDWNDTLKEVVTQLRGHFKNKTIIFKNHAGGDLEIVTHSFNCGGEFFYCNTTDLFNSTWDKDGNGTVSSGSNGTITLQCRIKQIINMWQRVGQAMYAPPIQGVIRCDSNITGLLLTRDGGNGNDSQANETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVQREKRAALGAVFLGFLGAAGSTMGAASLTLTVQARLLLSGIVQQQNNLLKAIEAQQHLLQLTVWGIKQLQARVLALERYLRDQQLLGIWGCSGKLICTTAVPWNASWSNKSMDYIWQNMTWMQWEREIDNYTELIYSLLEASQNQQEKNEQELLELDKWASLWNWFSISNWLWYIRIFIMIVGGLIGLRIVFTVLSIVNRVRKGYSPLSFQTRPPAPRGLDRPEGIEEEGGE",
    "response#2": "MWVTVYYGVPVWKEAKTTLFCASDAKAQDQEVHNVWATHACVPTDPNPQEMILGNVTENFNMWKNDMVDQMHEDVISLWDQSLKPCVRLTPLCVTLNCTNVSILWVTVHYGVPVWRDADTTLFCASDAKAHETEVHNVWATHACVPTDPNPQEIHLKNVIENFNMWKNKMAEQMQEDVISLWDQSLKPCVKLTPLCVTMNCTNAVFTNVTSNNSSIKIGNITDEVRNCSFHTTTEIRDKKQKSYALFYRLDIVPISDNDNNRSEYRIINCNSSVIKQACPKITFDPIPIHYCTPAGFALLKCNDKNYNGTGPCRNVSSVQCTHGIKPVVSTQLLLNGSLAEDEIVIRSENITNNAKTIIVHLNKSVEINCTRPSNNTRTSVTIGPGQVFYRTGDIIGDIRKAYCKINGTEWGKVLTQVKKKLEKHFNNKTIIFQPQPPSGGDPEITMHHFNCRGEFFYCNTSQLFNCTNNNGMCNSTIILPCKIKQIINMWQRVGQAMYAPPVRGRINCVSNITGILLTRDGGKTNSTNETFRPEGGNIKDNWRNELYKYKVIQIEPLGLAPTRARRRVVEREKRAVGLGMLFLGVLSTAGSTMGAASITLTAQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLALERYLKDPQLLGLWGCSGKLICTTNVPWNSSWSNKSLGVIWENMTWMQWEREISKYTGTIYTLLEESQNQQEINEKDLLALDSWKNLWSWFDISKWLWYIRIFIMIIGGLIGLRIIFAVLSIVNRVRQGYSPLSFQT",
    "response#3": "MRVMGIRKNYQHWWRWGTMLLGMLMICSAADQLWVTVYYGVPVWKEAKTTLFCASDAKAYEREVHNIWATHACVPTDPNPQEMVLGNVTENFNMWKNDMVDQMQEDIISLWDQSLKPCVKLTPLCVTLKCKNVNATNNGTKWVTVYYGVPVWKEAVTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDANLNCTNANLNCTNATSTSSNVTNNATSRGWGKLEAGEVQNCSFDVTTYRKDKMRKEYALFYKVDVVPISEDNSANNNSTNNTYKTYRLISCNTSVITQACPKVTFEPIPIHYCAPAGFAILKCNDKNFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSANFSDNAKNILVQLNESVEINCTRPHNNTRRSITIGPGRAFYATGAIIGDIRKAYCELDATKWKNTLKKVVGKLREQYKNKTITFQPHSGGDPEVVMHIFNCRGEFFYCNLTNLFDSTWNGTESNDTIGNGTVILPCRIKQIINMWQEVGKAMYAPPIAGLINCLSNITGLILTRDGGNTTNTTDTETFRPGGGDMRDNWRNELYKYKVVKVEPLGVAPTRAKRRTVQREKRAVTLGAMFLGFLGAAGSTMGAASIALTAQARQLLSGIVQQQSNLLRAIEAQQHMLRLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTNVPWNSSWSNKSQSEIWDNMTWLQWDKEISAYTDTIYNLLEKSQNQQEKNEQDLLALDKWANLWNWFDISRWLWYIKIFIMIVGGLIGLRIVFAVLSIINRVRQGYSPLSF"
  },
  {
    "reference": "MILDSELIIVATRNPGKVKEFEHAFAQLGKQVKSMFDYPELPEVVEDGVTFAENALKKAKTVGDALGLPVLADDSGLCVDELEGRPGVYSARFAGEHATDQQNNEKLLHMLEDKKLGEDTAQPLLSTARFVCSLVLYDPATGTHIEASGAVDGWITDEPAGCGGFGYDPLFYLPDYEKTMAELSMEEKQAISHRGVALRSLINMLQQQPEM",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must be able to ribonucleoside triphosphate phosphatase activity, dITP diphosphatase activity. The designed protein sequence is ",
    "response#1": "GHAE",
    "response#2": "GCRIS",
    "response#3": "MP"
  },
  {
    "reference": "MRLSRYFLPILRETPKEAEIVSHRLMLRAGMIRQEAAGIYAWLPLGLRVLNRVTEVIRAEQDRSGAIELLMPTIQSAELWRESGRYEAYGKEMLRIRDRHEREMLFGPTNEEMITAIFRSAVRSYKDLPKNLYHIQWKFRDEVRPRFGTMRSREFLMKDAYSFDLDEAGARHAYNKMFVAYLRTFARLGLKAIPMRAETGPIGGNLSHEFIILAQTGESEVFCDRAYLGFPIPPASIDFDDVAALQATVDHWTSRYAATSEMHEPAAFEAVPEEARMAARGIEVGHIFYFGTKYSEPMGARVTGPDGQERPVHMGSYGIGPSRLVAAIIEASHDEAGIVWPDSVAPFDVALLNLKVGDAATDAACAKLQEELEAAGLTVLTDDRDERPGAKFATADLIGLPWQVIVGPKGLAEGTIELKRRATGEREIVAVDAVAPRLRGAKE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein localizes to the cytoplasm.2. The protein must exhibit the following characteristics: Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).. The designed protein sequence is ",
    "response#1": "TPGQRTIESVSEFLNLPAEQTVKTLLVLGEAEEGKPAPIIALVLRGDHVLNEIKAEKLPGVASPLTFASDEAIEAAVGCKPGSIGPVNLPLRTIVDNSAAVLANFVCGANEEGQHLTGVNWGER",
    "response#2": "VGIVNLLTTNEDCKTTAETIYAALADSSLYDDSTDSPGVKLARMDLLGMPWQVIIGNSFVKDKVVELKNRANGATERCTVDALIARMQ",
    "response#3": "VGIVNLLTTNEDCKTTAETIYAALADSSLYDDSTDSPGVKLARMDLLGMPWQVIIGNSFVKDKVVELKNRANGATERCTVDALIARMD"
  },
  {
    "reference": "MEPPQCVEELEDDVFQPEDGEPGTQPGSLLSADLFAQSQLDCPLSRLQLFPLTHCCGPGLRPVSQEDKATQTLSPASPSQGVMLPCGVTEEPQRLFYGNAGYRLPLPASFPAGSALGEQPPEGQFLQHRAEVQIARKLQCIADQFHRLHMQQHQQNRDRAWRQVFLFLQNLALNRRENREGVGPW",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Incorporating a BH3 motif is critical for the successful design of the protein.2. For general function, the protein need meet that May play a role in apoptosis.3. The protein should have a specific function in negative regulation of apoptotic signaling pathway, positive regulation of apoptotic process, positive regulation of release of cytochrome c from mitochondria, negative regulation of autophagic cell death and should be localized to cytoplasm, actin cytoskeleton, acrosomal vesicle in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "LRRAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGC",
    "response#2": "WLGDELEYLRPNVYRNVARQLNITIASENIVSDAFLAVAAEIFSTEYSRKGLEKHKGVTWGKIVSLYAVAGALAVDCVRNGHPAMVHTIVDCMGEFVRKSLASWLKKRGGC",
    "response#3": "WLGDELEYLRPNVYRNVARQLNITIASENIVSDAFLAVAAEIFSTEYSRKGLEKHKGVTWGKIVSLYAVAGALAVDCVRNGHPAMVHTIVDCMGEFVRKSLASWLKKRGGC"
  },
  {
    "reference": "MSETAPAASSTLVPAPVEKPATKRRGKKPGMATARKPRGFSVSKLIPEALSMSQERAGMSLAALKKALAAAGYDVEKNNSRIKLALKRLVNKGVLVQTKGTGASGSFKLSKKAASGNDKGKGKKSASAKAKKLGLSRASRSPKSSKTKVVKKPKATPTKGSGSRRKTKGAKGLQQRKSPAKARATNSNSGKSKMVMQKTDLRKAAGRK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Testis-specific histone H1 that forms less compacted chromatin compared to other H1 histone subtypes.2. The designed protein should be involved in cell differentiation, negative regulation of DNA recombination, binding of sperm to zona pellucida, chromosome condensation by localizing to nucleosome, condensed nuclear chromosome and exhibiting double-stranded DNA binding, DNA binding, nucleosomal DNA binding, structural constituent of chromatin.3. The protein contains novel H15 domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "GYLAQHQLFDQIPELKRDISIPDYCCLGNGEEEEITINAWFGPQGTISPLHQDPQQNFLVQVLGRKYIRLYSPQESEAVYPHETHILHNTSQVDVENPDLEKFPKFTEAPFLSCILSPGDTLFIPAKYWHYVRSLDLSFSVSFWWR",
    "response#2": "GYLAQHQLFDQIPELKRDISIPDYCCLGNGEEEEITINAWFGPQGTISPLHQDPQQNFLVQVLGRKYIRLYSPQESEAVYPHETHILHNTSQVDVENPDLEKFPKFTEAPFLSCILSPGDTLFIPAKYWHYVRSLDLSFSVSFWWR",
    "response#3": "GYLAQHQLFDQIPELKRDISIPDYCCLGNGEEEEITINAWFGPQGTISPLHQDPQQNFLVQVLGRKYIRLYSPQESEAVYPHETHILHNTSQVDVENPDLEKFPKFTEAPFLSCILSPGDTLFIPAKYWHYVRSLDLSFSVSFWWR"
  },
  {
    "reference": "MEKFQSYLGLDRSQQHYFLYPLIFQEYIYVLAHDHGLNRSILLENAGYDNKSSLLIVKRLITRMYQQNHLILSVNDSKQTPFLGDNKNFYSQVMSEVSSIIMEIPFSLRLISSLERKGVVKFDNLRSIHSIFSFLEDNFSHLNYVLDILIPYPAHLEILVQALRYWIEDASSLHLLRFFLHEYHNRDSLITSNSKKASSSFSKRNHRLFFFLYTSHVCEYESGFIFLRNQSSHLRSTSSGALLERIYFYGKIEHLAEVFTRAFQANLWLFKDPFMHYVRYQGKSILASKGTFLLMNKWKYYFVNFWKSYFYLWSQPGRIYINQLSNHSLDFLGYRSSVRLKPSMVRSQMLENAFLIDNAIKKFDTIVPIMPLIGSLAKSKFCNALGHPIGKAIWADLSDSDIIDRFGRIYRNLSHYHSGSSKKKSLYRVKYILRLSCARTLARKHKSTVRAFLKRFGSELLEEFFTEEEQVFSLTFPRVSSISRRLSRRPIWYLDIICINDLANHE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must possess RNA binding to support RNA splicing under certain conditions. The designed protein sequence is ",
    "response#1": "PKKAVGKFSELWVNYAKFYERGGDLDTARIIFEKAVKVPFKSVNELAETWCEWAEMELRSENFDKAVEIMAKATQAPKKSTVDYFDETLSPQQRIHKSWKLWSFYVDLVESVSSIEETKKVYERIFELRIATPQTVVNYANLLEEHKYFEESFKVYERGLDLFTYPVAFELWNLYLTKAVDRKIGIERLRDLFEQALDGCPPKFARPLYLMYGNLEEERGLARHAMRIYERATRAVSDEDRFEMFEFYITKSASNFGLPSTRPIYERAIAALPDHEAKEMCLKFAEMERRLGEIDRARAIYGHASQFCDPRTNAPFWQKWEAFEVQHGNEDTFKEMLRIKRSVQAQYNTDVNFIASD",
    "response#2": "PKKAVGKFSELWVNYAKFYERGGDLDTARIIFEKAVKVPFKSVNELAETWCEWAEMELRSENFDKAVEIMAKATQAPKKSTVDYFDETLSPQQRIHKSWKLWSFYVDLVESVSSIEETKKVYERIFELRIATPQTVVNYANLLEEHKYFEESFKVYERGLDLFTYPVAFELWNLYLTKAVDRKIGIERLRDLFEQALDGCPPKFARPLYLMYGNLEEERGLARHAMRIYERATRAVSDEDRFEMFEFYITKSASNFGLPSTRPIYERAIAALPDHEAKEMCLKFAEMERRLGEIDRARAIYGHASQFCDPRTNAPFWQKWEAFEVQHGNEDTFKEMLRIKRSVQAQYNTDVNFIASR",
    "response#3": "KKLETRVPIIPMIESLSKAKFCNALGNPISKPTWADSSDSDIIDKFLRICKNLSRYHSGSSKKKTLYRVKYILRLSCLKTLARKHKTTGRVFLKQLGSEVFEEFLRREEC"
  },
  {
    "reference": "MGGKWSKSSVVGWPTIRERMKRAEPAADGVGAVSRDLEKHGAITSSNTAANNADCAWLAAQEDEEVGFPVRPNVPLRPMTFKAAVDLSHFLKEKGGLDGLIYSQKRQDILDLWVYHTQGFFPDWQNYTPGPGTRFPLTFGWCYKLVPVDPAKVEEENKGENNSLLHPMSLHGMEDPEREVLMWKFDSRLAFHHVARELHPEYYKNC",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should contain at least one Dileucine internalization motif; necessary for CD4 internalization motif to achieve its intended function.2. The designed protein should have a specific function within the host cell Golgi membrane, host cell plasma membrane. The designed protein sequence is ",
    "response#1": "MPA",
    "response#2": "SCGAVR",
    "response#3": "MN"
  },
  {
    "reference": "MTSEIHMPGPMCLIENINGQLLVNQEALKILSAIKQPVVVVAIVGLYRTGKSYLMNKLAGKKKGFSLGSTVQSHTKGIWMWCVPHPKKPDHALVLLDTEGLGDVEKGDNQNDSWIFALAILLSSTFVYNSMGTINQQAMDQLHYVTELTDRIRAKSSPDANEVEDSSDFVSFFPDFVWTLRDFSLDLEVDGQAISADQYLENSLKLKHGTGEKIKNFNLPRLCIRMFFPKKKCFIFDRPTHRKKLGQLETMHDDELDPEFVRQVAEFCSYIFSNSKTKTLSGGIRIDGPRLENLVLTYVSAISSGDLPCMENAVLALAEIENSAAVRKAIAHYDQQMSQKVQLPTETLQELLDLHRTSEREAIEVFIKNSFKDVDHSFQKELAAQLEKKQNDFVNQNMEASSERCSALLQDIFQPLEESMKQGVFSKPKGYRLLIQRMQELKEQYYQAPGKGIQAEETLQKYLKSKESAIDAILQTDQTLTEKEKDIEVERVKAEAAEANVKMLEELQKKNQQMMEQKEKSYQEHVKQLTDKMERDRVQMLQEQERTLALKLQEQARLMNEGFANESKRLTNEIESLRRQMRPPRRRCVIS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have GDP binding to facilitate cellular response to tumor necrosis factor.2. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "VVWCAVGPEELRKCRQWSSQSSQNLNCSLASTTEDCIVQVLKGEADAMSLDGGFIYTAGKCGLVPVLAENQKSRQSSSSDCVHRPTQGYFAVAVVRKANGGITWNSVRGTKSCHTAVDRTAGWNIPMGLLVNQTGSCKFDEFFSQSCAPGSQPGSNLCALCVGNDQGVDKCVPNSNERYYGYTGAFRCLAENAGDVAFVKDVTVLDNTNGQNTEEWARELRSDDFELLCLDGTRKPVTEAQNCHLAVAPSHAVVSRKEKAAQVEQVLLTEQAQFGRYGKDCPDKFCLFRSETKNLLFNDNTEVLAQLQGKTTYEKYLGSEYVTAIANLI",
    "response#2": "VVWCAVGPEELRKCRQWSSQSSQNLNCSLASTTEDCIVQVLKGEADAMSLDGGFIYTAGKCGLVPVLAENQKSRQSSSSDCVHRPTQGYFAVAVVRKANGGITWNSVRGTKSCHTAVDRTAGWNIPMGLLVNQTGSCKFDEFFSQSCAPGSQPGSNLCALCVGNDQGVDKCVPNSNERYYGYTGAFRCLAENAGDVAFVKDVTVLDNTNGQNTEEWARELRSDDFELLCLDGTRKPVTEAQNCHLAVAPSHAVVSRKEKAAQVEQVLLTEQAQFGRYGKDCPDKFCLFRSETKNLLFNDNTEVLAQLQGKTTYEKYLGSEYVTAIANLL",
    "response#3": "VVWCAVGPEELRKCRQWSSQSSQNLNCSLASTTEDCIVQVLKGEADAMSLDGGFIYTAGKCGLVPVLAENQKSRQSSSSDCVHRPTQGYFAVAVVRKANGGITWNSVRGTKSCHTAVDRTAGWNIPMGLLVNQTGSCKFDEFFSQSCAPGSQPGSNLCALCVGNDQGVDKCVPNSNERYYGYTGAFRCLAENAGDVAFVKDVTVLDNTNGQNTEEWARELRSDDFELLCLDGTRKPVTEAQNCHLAVAPSHAVVSRKEKAAQVEQVLLTEQAQFGRYGKDCPDKFCLFRSETKNLLFNDNTEVLAQLQGKTTYEKYLGSEYVTAIANLL"
  },
  {
    "reference": "MKRSSVSTCGAGRLSMQELRTLDLNKPGLYTPQTKERSTFGKLSTHKPTSERKVSIFGKRTSGHGSRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLYEFLTENGYVYSVSMKSLQAPSTKEFLKIFAFLYGFLCPSYELPGTKCEEEVPRIFKALGYPFTLSKSSMYTVGAPHTWPHIVAALVWLIDCIKIDTAMKESSPLFDDGQLWGEETEDGIKHNKLFLEYTKKCYEKFMTGADSFEEEDAELQAKLKDLYKVDASKLESLEAENKALNEQIARLEEEREREPNRLMSLKKLKASLQADVQNYKAYMSNLESHLAVLKQKSNSLDEEIGRVEQECETVKQENTRLQSIVDNQKYSVADIERINHEKNELQQTINKLTKDLEAEQQQMWNEELKYARGKEAIEAQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRTQVYAPLKELLNESEEEINKALNKKRHLEDTLEQLNTMKTESKNTVRMLKEEIQKLDDLHQQAVKEAEEKDKKSASELESLEKHKHLLESGVNDGLSEAMDELDAVQREYQLTVKTTTEERRKVENNLQRLLEMVATHVGSLEKHLEEENAKADREYEEFMSEDLLENIREMAEKYKRNAAQLKAPDK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules; Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.; Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.2. A protein that can perform kinetochore adaptor activity in Ndc80 complex, nucleus, outer kinetochore to support spindle assembly involved in female meiosis I. The designed protein sequence is ",
    "response#1": "RKTSFFGKRMSNGTARTSQYGAFGGTEKIKDPRPLHDKAFIQQCIRQLCEFLNENGYSQTLTVKSLQGPSTKDFLKIFAFIYTFICPNYENPESKFEEEIPRIFKELGYPFALSKSSMYTVGAPHTWPQIVAALVWLIDCVKLC",
    "response#2": "RKTSFFGKRMSNGTARTSQYGAFGGTEKIKDPRPLHDKAFIQQCIRQLCEFLNENGYSQTLTVKSLQGPSTKDFLKIFAFIYTFICPNYENPESKFEEEIPRIFKELGYPFALSKSSMYTVGAPHTWPQIVAALVWLIDCVKLC",
    "response#3": "RKTSFFGKRMSNGTARTSQYGAFGGTEKIKDPRPLHDKAFIQQCIRQLCEFLNENGYSQTLTVKSLQGPSTKDFLKIFAFIYTFICPNYENPESKFEEEIPRIFKELGYPFALSKSSMYTVGAPHTWPQIVAALVWLIDCVKLA"
  },
  {
    "reference": "MGGKWSKSSIVGWPAVRERIRQTEPRTEPAAEGVGAASQDLDKYGALTSSNTAHNNADCAWLQAQEEEEGVGFPVKPQVPLRPMTYKAAFDLSFFLKEKGGLDGLIWSRKRQEILDLWVYHTQGFFPDWQNYTPGPGVRLPLTFGWCYKLVPVDPSEVEEANKGENNCLLHPMSQYGMEDEEREVLKWQFDSSLARRHLAREKHPEYYNDC",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein's functional design necessitates the inclusion of a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif.2. For general function, the protein need meet that Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis3. A protein that localizes to extracellular region, host cell plasma membrane and can perform SH3 domain binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "MEPVDPSLEPWNHPGSQPKTACNNCYCKRCSYHCLVCFQKKGLGISYGRKKRRQRRSAPPSSEDHQNLVSKQPLPQTQGDSTGSEESKKKVESKTEADPFD",
    "response#2": "MGGKWSKSSLVGWPAIRERIR",
    "response#3": "GHVTV"
  },
  {
    "reference": "MNMRNIALWAVIAALLIALFSYLQPQDNAAARNEISYSRLLNEAQDGRIASIDVDQFDITGTYKTGEKFTSRAYSHEHLSAAMQEAGVDVSFEDPRGGFNIWSVVSSILPLIIIVGLWFFLMRQMQGGGGRGAMSFGKSKARLLTERQGRVTFDDVAGIDEAKEELQEIVEFLQDPSKFQRLGGKIPKGALLVGPPGTGKTLTARAVAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRSRGAGIGGGNDEREQTLNQLLVEMDGFEANEGIIIIAATNRPDVLDPALLRPGRFDRQITVGNPDIIGREKILKVHMRNVPTAKDVNARVIARGTPGFSGADLANLVNEAALLAARRGKRMVSQREFDDAKDKVLMGPERRSMVMSDDEKQLTAYHESGHAIVSIFMPACDPIHKATIIPRGRALGMVQYLPEDDKHSHKRVQMTSRLAMAMGGRVAEELKFGEENVTSGAMGDIQMATQMARAMVTQFGLSDEIGPIDYGDSGDPYRPKHISEQTARKIEEEVRKLVQTGMDEARRILTEHNDEWEQVAQALLEYETLTGDEIQDLLKGIKPQRPEDTDDTPGPSSAVPITGSKKKKDTPDGDVGDGSPEPA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must bind to Zn(2+) in order to perform its enzymatic function.2. The presence of AAA+ ATPase domains is critical for the interaction of the designed protein with its intended targets.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.4. For general function, the protein need meet that Plays a role in the quality control of integral membrane proteins.; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins5. The designed protein must be optimized for zinc ion binding, metalloendopeptidase activity, ATP binding.6. The designed protein must have a active site that is structurally stable and resistant to denaturation.7. The designed protein should have a binding site that is specific to the target ATP ligand. The designed protein sequence is ",
    "response#1": "VLLVGPPGTGKTLLAKAIAGEAGVPFFSMSGSEFVEMFVGVGASRVRDLFRKAKESAPCIVFIDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNSGVIIIAATNRPDVLDSALMRPGRFDRQITVHLPYLEDTEKMGIHMLVEKMHFRLPEEVSIEAIARRTPGFAGADLANLLNEAAILTARRRKPAISLEEIENLLDRAYKRSVKFGRGPVFDEYDEEERKMVAYHEAGHAVIGIKLKHAPKVQKITIIPRGDAGGYNLMLPEKETIFSSKKRMLANIVAFLGGRAAEELMFDDISNGTYSDFQQATSIANEMVTSYGMSELGPMQNDDSLFVHKPLVDAEVKKIIDECMDEARKIMKENKSLLEQIANTLLEQETITKDQLDQLREHYESQ",
    "response#2": "VLLVGPPGTGKTLLAKAIAGEAGVPFFSMSGSEFVEMFVGVGASRVRDLFRKAKESAPCIVFIDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNSGVIIIAATNRPDVLDSALMRPGRFDRQITVVSLTPMNLEDRLKILSLYKDSLDIDVSPVELERIAKQTPGFSGADLANLMNEAAILTARRNKKIIENFISKVKEI",
    "response#3": "MVMSEKEKLNTAYHESGHAIVGRLMPEHDPVYKVSIIPRGRALGVTMFLPEEDKYSHSKRYLDSSICSLFGGRIAEELTLGFEGVTTGASNDIERATSLARNMVTRWGLSEKLGPLQYDTDSDEPFLGRSAGQAQTVYSPETAQRIDEEVRNIIDTCYEKAKQILVDNRDKLDLMADALMKYETIDRFQIDDICATPCDNA"
  },
  {
    "reference": "MAKAISLEEIKNETVDLEKIPIEEVFEQLKCTREGLSADEGASRLQIFGPNKLEEKNESKILKFLGFMWNPLSWVMEAAAVMAIALANGDGKPPDWQDFIGIICLLVINSTISFIEENNAGNAAAALMAGLAPKTKVLRDGRWSEQEAAILVPGDIISVKLGDIIPADARLLEGDPLKIDQSALTGESLPVTKNPGDEVFSGSTCKQGELEAVVIATGVHTFFGKAAHLVDSTNNVGHFQKVLTAIGNFCICSIAIGMLVEIIVMYPIQHRKYRDGIDNLLVLLIGGIPIAMPTVLSVTMAIGSHRLSQQGAITKRMTAIEEMAGMDVLCSDKTGTLTLNKLSVDRNLVEVFAKGVDKEYVLLLAARASRVENQDAIDACMVGMLADPKEARAGIREVHFLPFNPVDKRTALTYIDNNNNWHRASKGAPEQILDLCNAKEDVRRKVHSMMDKYAERGLRSLAVARRTVPEKSKESPGGRWEFVGLLPLFDPPRHDSAETIRRALNLGVNVKMITGDQLAIAKETGRRLGMGTNMYPSASLLGQDKDSAIASLPIEELIEKADGFAGVFPEHKYEIVKKLQERKHIVGMTGDGVNDAPALKKADIGIAVADATDAARGASDIVLTEPGLSVIISAVLTSRAIFQRMKNYTIYAVSITIRIVFGFMFIALIWKYDFSAFMVLIIAILNDGTIMTISKDRVKPSPMPDSWKLKEIFATGVVLGGYQALMTVVFFWAMHDTDFFSDKFGVKSLRNSDEEMMSALYLQVSIIS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.2. The designed protein should contain Extracellular, Cytoplasmic topological domains.3. The designed protein is the optimization of the 4-aspartylphosphate intermediate to achieve high catalytic efficiency for ATP + H(+)(in) + H2O = ADP + 2 H(+)(out) + phosphate.4. The designed protein should have P-type proton-exporting transporter activity, ATP hydrolysis activity, metal ion binding, ATP binding within plasma membrane to regulate proton export across plasma membrane.5. For general function, the protein need meet that The proton gradient it generates drives the active transport of nutrients by H(+)-symport; The resulting external acidification and/or internal alkinization may mediate growth responses.; The plasma membrane ATPase of plants and fungi is a hydrogen ion pump6. The protein should contain Helical; Name=8, Helical; Name=5, Helical; Name=9, Helical; Name=7, Helical; Name=4, Helical; Name=6, Helical; Name=10, Helical; Name=3, Helical; Name=1, Helical; Name=2 transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "MKKVAGTLRLDLAQYRELEAFAKFGSDLDKATQAQLTRGGRLVEILKQGQYVPMAVEKQVAILFVGTQGILDTLPANLVRKFEVAFLEMLELKHKDILNTIATAGQMDADTQKKLRQVAEQFLETLEREQAQFLGQRVIDTFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPELIDPTTGQSIMQRTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRVKTLGSTAREGSITAIGAVSPPGGDISEPVTQNTLRIVKVFWGLDAQLAQRRHFPAINWLSSYLYIDKTGTLPPAIDAIVTRMSAVEEMAGLDVLCSDKTGTLTLNKLSIDPSNVFPVGTMDIPEVMKFGALSANIITEEPIDMVLWESYPEREKLKSEYKHTKYFPFNPNDKITIATVLEIATGRVFRVLKGSPQVVLAKAWNAQALDGPVNEKIKEYAGRGFRSLGIAMAEGDGKDGTKWEMLAVLPMFDPPRHDTKETIERCMKQGIAVKMVTGDHLLIGKETAKMLGMGTEMYPSEVLIKARNGDVEAPHGYKNYVAMVEACNGFAQVFPEHKFEIVEILQEAHHRVGMTGDGVNDAPALKKAHVGVAVADATDAARGAADIVLTEPGLSTIVTAVIGARG",
    "response#2": "MKKVAGTLRLDLAQYRELEAFAKFGSDLDKATQAQLTRGGRLVEILKQGQYVPMAVEKQVAILFVGTQGILDTLPANLVRKFEVAFLEMLELKHKDILNTIATAGQMDADTQKKLRQVAEQFLETSQGQRVIDTFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPELIDPTTGQSIMQRTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRVKTLGSTAREGSITAIGAVSPPGGDISEPVTQNTLRIVKVFWGLDAQLAQRRHFPAINWLSSYKGLM",
    "response#3": "MKKVAGTLRLDLAQYRELEAFAKFGSDLDKATQAQLTRGGRLVEILKQGQYVPMAVEKQVAILFVGTQGILDTLPANLVRKFEVAFLEMLELKHKDILNTIATAGQMDADTQKKLRQVAEQFLETLGQRVIDTFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPELIDPTTGQSIMQRTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRVKTLGSTAREGSITAIGAVSPPGGDISEPVTQNTLRIVKVFWGLDAQLAQRRHFPAINWLSSYEHVVIKIPMTKKGAITKRMTAIEEMAGMDVLCSDKTGTLTLNKLSVDKNLIEVFKRGIDRDMAVLMAARAARLENQDAIDTAIVSMLSDPKEARAGIKELHFLPFSPANRRTALTYLDGEGKMHRVSKGAPEEILDMAHNKLEIKEKVHATIDKFAERGLRSLGLAYQEVPDGDVKGEGGPWDFVALLPLFDPPRHDSAQTIERALHLGVSVKMITGDQLAIAKETGRRLGMGTNMYPSSSLLSDNNTEGVSVDELIENADGFAGVFPEHKYEIVKRLQSRKHICGMTGDGVNDAPALKKADIGIAVDDATDAARGASDIVLTEPGLSVIISAVLTSRAPNLDREAKWDFVPGLSVGDAVEAGDVLGTVQETNLVEHRIMVPVGVSGRLANISAGSFTVEETVYEIEQADGSVFKGTLMQKWPVL"
  },
  {
    "reference": "MKKKSIKDKEQENNWEEKVVQVKRVTKVVKGGKKLSFRAILIVGNEKGEIGVGIGKASDVIGAVKKGVTDAKKHIINVPLTKSYSIPHPIEGISGAAKVILRPSAIGSGVIAGGSTRTVLELAGVKNILAKQLRSSNTLNNARAVLNALSQLRTFQNTAQNRDINIERLFNM",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: With S4 and S12 plays an important role in translational accuracy..2. A protein that localizes to chloroplast, small ribosomal subunit and can perform rRNA binding with high specificity and efficiency.3. The presence of S5 DRBM domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MTPLARLDNVVYRMGFGSTRAEGRQLVSHKAFTVNGIVVNIASYQVKPGDVIAVREKSKKQVRIVEALN",
    "response#2": "MLARLDNVVYRMGFGSTRAEGRQLVSHKAFTVNGIVVNIASYQVKPGDVIAVREKSKKQVRIVEALN",
    "response#3": "MRRR"
  },
  {
    "reference": "MNKMEQMKQQLREEIVVAVKAAGLATDETIPEVILETPKEKAHGDFATNMAMQLARVAKKAPRLIAEELTAKLDRKRAAIEKIEIAGPGFINFFLDNSYLREMIPTVLRAESAYGETNVGKGKKVQVEFVSANPTGNLHLGHARGAAVGDALCNILAKAGYDVSREYYINDAGNQINNLALSLEARYFQALGMEKDMPEDGYHGEDIIQFGKDLAETHGDKFVHESSEERLAFFREYGLKRELEKIKSDLEEFRVPFDNWYSETSLYTTGKVEKTLNVLKEKGKTYEQDGALWFRSTEYGDDKDRVLVKNDGSYTYLTPDISYHEDKFLRGFEKLINIWGADHHGYIPRMKAAIQALGYEKDQLDVQIIQMVSLFQNGEKVKMSKRTGKAVTLRDLMEEVGIDATRYFFAMRSADSHLDFDMDLAVSKSNENPVYYVQYAHARVCSMLRKGKELGLSFDESTDLSPIASEKEYDLLKKIGEFPEVVAEAAQKQMPHRITNYVHELASTLHSFYNAEQVINPENDEQSKARLALMKATQVTLKNALSLVGVEAPERM",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must be able to regulate arginyl-tRNA aminoacylation. The designed protein sequence is ",
    "response#1": "QYATARCHSILNQARTKKITPLVKETNLLNNPKEIELLLVLDNFKEVIKNSANNRSTQQICDYIQNICKIFHSYYAEIKIIDENNLQLTKLRLGFIKAVLQVLENAFFIIGIQPVLED",
    "response#2": "EANKRVSNILAKSADVKLNDKVLASVLKAPEEVQLAANLSVLQDKLAPLFAERKYQEALVELASLRDVVNNFFDKVMVMDKDEEIRVNRLTMLHELRELA",
    "response#3": "VQYAHARICSILRMAKERGIELPSFENVEPQLLRIPEEIALIKTLTRFPEVVEGSARTLEPHRITFYLNDLAGLFHSYYNKYKVISDDEAMTRTRLFLVKCIQTVLKNALTLLGVSAPEKC"
  },
  {
    "reference": "MSHQSDLIAEDILAYLAQHERKELLRFLTCGNVDDGKSTLIGRLLHDSKMIYEDHLEAITKDSKKVGTTGDEVDLALLVDGLQAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRNMATGASTCDLAIILIDARYGVQTQTRRHSYIASLLGIRHIVVAINKMDLLGFDQGVFEKIKADYLAFADQIGLKPSSLFFVPMSALKGDNVVNRSEQAPWYDGQSLMEILETVEISGDRNLSDMRFPVQYVNRPNLNFRGFAGTLASGIVRKGDEVVALPSGKGSKIKSIVTYEGELEQAGPGEAVTLTLEDEIDVSRGDMLVHADNRPQVTENFEAMLVWMAEEPMLPGKKYDIKRATSYVPGSIPSILHRVDVNTLEQTAASALQLNEIARVKVSLDAPIALDGYEYNRTTGAFIVIDRMTNGTVGAGMIIAAPVVGQNASVHHGALAHVATEERAARFGQQPATVLFTGLSGAGKSTLAYALERKLFDAGRAVYVLDGQNLRHDLNKGLSPDRAGRTENWRRAAHVAKQFNEAGLIALAAFVAPDAEGREQAKALIGAERLITVHVQASLAACRTRDPQGLYAAGGDNIPGESFPYDLPVDADLVIDTEQTSVEQGVKEVLALLRQRGLI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be optimized for activity in hydrogen sulfide biosynthesis, sulfite from sulfate: step 2/3 pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The GTP binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein should be optimized for ATP binding, adenylylsulfate kinase activity, GTPase activity activity in order to improve sulfate assimilation, hydrogen sulfide biosynthetic process. The designed protein sequence is ",
    "response#1": "KSLLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALENDSKNVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFTTDKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSYLVKLLGIPNVVLAINKMDLVDYSASTFHQIVADYSAFAESIGLNAFVPVPISGLKGDNIVEKSDAMPWYQGPTLLAHLESVPLGDTSAA",
    "response#2": "KQLLRFITCGSVDDGKSTLIGRLLYDSKMIYEDQLAQIEADSKIVGTTGGKFDPALLTDGLKAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRNMATGASSADLAILMIDARHGVLTQTKRHSFIVSLLGIRHIVVAINKMDLIDYDEQKFEAICDDYRNFVTRLDLPDLHFIPISALDGDNVVERSEKMPWYTGSTLMNFLETVYIGSDRFITCGSVDDGKSTLIGRLLHDTKQIFDDQVTALQRDSRKHGTQGGEVDLALLVDGLQAEREQGITIDVAYRFFSTDRRSFIVADTPGHEQYTRNMATGASTADLAVILVDARHGLTRQSRRHALLVSLLGIRRVALAINKMDLVGWSQDKFEAIVSGFQAFAAPLNFTEVRAIPLSAKNGDNVVLPGTAATWYTDVPLLRYLEEVPVKR",
    "response#3": "KQLLRFITCGSVDDGKSTLIGRLLYDSKMIYEDQLAQIEADSKIVGTTGGKFDPALLTDGLKAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRNMATGASSADLAILMIDARHGVLTQTKRHSFIVSLLGIRHIVVAINKMDLIDYDEQKFEAICDDYRNFVTRLDLPDLHFIPISALDGDNVVERSEKMPWYTGSTLMNFLETVYIGSDHVTRHFEAMLVWMSSQKLRVGGSYHLKHTTQSLRAKVEALRYVVDVNTLSRDKEADGLGLNGIGRVVLSTTRPLYVDAYLDNRATGSLILIDALSNETVAAGFRLPVQYVNRPNLDFRGFCGTIASGKVRKGDEVLALPSKRRSKVKSIVTFDGELDEAFAPLAVTLTLEDEIDVSRGDMLVRPGQEIADQFEANLLWLNKNAMIPGRQYLMKLACKEVTATVTGIKYRQDSQTGARLATKTLNLNEAGRVSLSTGAPLIFDRHGKNRHLNSFLLIDRQSLETVGIGMVHFGLRRAQNIHWQALDIHREARAALKGQKPAVLWFTGLSGSGKSTIANLVEKRLHAAGKHTYLLDGDNVRHGLNRDLGFTDADRVENIRRVAEVARLMTDAGLIVLTAFISPFRAERQMARELLEDGEFIEVFVDTPLEVAEKRDPKGLYKKARRGELRNFTGIDSPYEPPERAEIHIDTTRTPPEEAADRIVGWLAARGVIG"
  },
  {
    "reference": "MIQVNEYFDGTVKSLGYESPEGKSTIGVMEKGEYEFNTSQKEYMTVIEGELVVLLPEATAWKSFKKNETFSVDAHLSFKVKANEQSSYLCKYA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates; Catalyzes the phosphorolysis of diverse nucleosides, yielding D-ribose 1-phosphate and the respective free bases. The designed protein sequence is ",
    "response#1": "MPK",
    "response#2": "MTSPS",
    "response#3": "SPRI"
  },
  {
    "reference": "MNNKKPDIKSMDLTELTEFITAAGQKPFRGRQLYQWMHQKCATSIDEMTNLPKAFRDELKEGCTYTVLKQANVQASTQDGTRKYLFLLEDGNMVESVFMRYKTGNSVCISSQAGCRMGCKFCASAIDGLSRNLTPSEMLEQVYQISRDTGERVSNVVVMGTGEPLDNYDNLLQFLTLLCNEDGIHMSQRNVTVSTCGLVPQILELAHKKLQITLAISLHAASQQKRCSLMPVANRYGIEDLMAACRYYFEMTGRRITFEYSLFAGVNDSKEDATMLCGLLSGMNCHVNLIPVNPVKGREFVPPGKEGLLSIKKHLEQKGIPVTVRRTLGSDIDAACGQLRKKYMEIAEKSPLS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein with the reaction adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine, the Proton acceptor, S-methylcysteine intermediate must be optimized to ensure efficient catalysis.2. The presence of [4Fe-4S] cluster is necessary for the protein to function in the desired environment.3. A protein that contributes to rRNA base methylation by functioning in cytoplasm with tRNA (adenine-C2-)-methyltransferase activity, rRNA (adenine-C2-)-methyltransferase activity, rRNA binding, tRNA binding, metal ion binding.4. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs..5. The S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions.6. The designed protein must have at least one Radical SAM core domain. The designed protein sequence is ",
    "response#1": "EGLGEPSYRGGQILEWLWQKRATSIEGMSNLPVRLREHLEESFTLRSLSPAKSQGSRDSTRKFLFRLNDGRYIECVLIPASPALYGERSDRRTLCVSSQVGCAFGCKFCASGLDGFTRNLTADEIAGQVLLAEELSGEKVNNIVFMGMGEPLANLPQLLKALELITSPWGLGIGARHLTVSTSGLVPQIRKLADHPQQIRLAISLHGASNDVRDRIMPVNRKWPVEDLFKALSYWTKRKNQKITLEYILIDGINADPAQARLLAQHAESLRAKVNLIPYNTVDGLKWKRPPEEECHAFRDILAARGIPATLRLEKGHDIDAACGQLRLKEERAEKEA",
    "response#2": "QEYGMSVCVTTQVGCNIGCTFCASGLLKKQRDLTAGEIVAQIMLVQRYFDERGKDERVSHIVVMGIGEPFDNYNNLMDFLHIINDAKGLAIGARHITVSTSGLVPKIKEFAKNGLQVNLAISLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLKGVNDRPEHAQQLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSKREDVMKFYDILKKNGVNCVIRKEHGTEVTSSQD",
    "response#3": "MKNSNPSIYSLELHELEDWLKEHGEKSFRANQVFEWLYTKRATSFEDMSNLSKSLRTLLNENFTLTTLNTLIQQTSKDGTIKFLFELHDGYSIETVLMRHEYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVKVQQALDEFEERVSHVVIMGIGEPFDNYDEMMSFLKIINHDKGLNIGARHITVSTSGIIPKIYKFADEKLQINFAISLHAPNTELRTKLMPINKAYKLPDLMEAVRYYVDKTGRRVSFEYGLFGGENDQVEHAEELAQLVKGLKCHINLIPVNYVPERNYVRTPKEQIKLFEDTLKKHGVNVTTRREQGHDIDAACGQLRAKERKEEE"
  },
  {
    "reference": "MDTRWLRNSFVYLVIMVAVLAIFFTLVNPAPRETQIPITELMTIVQRDAANNRVDKIVVDGNTLIADTSDGKKTAVKPDRSDIMEMLQARGIKDEQVNVEVRQPGRLGPWLGLIGSFLPVIIFGALLLFMMRQAQGSNNQALSFGKSRARLFMSNKPTITFADVAGVEEAKQELAEVVEFLKYPEKFAALGARIPRGVLLVGPPGTGKTYLSRACAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFEQAKRNSPCIVFVDEIDAVGRQRGAGLGGSHDEREQTLNQILVEMDGFDTNTNVIVLAATNRPDVLDPALLRPGRFDRRVVIDNPDVRGREAILDIHARGKPLAPDVGLDRLAKQTPGFSGADLNNLLNEAAILAARRDMQAITMSELEEAIDRVILGPERKSRVISDRERRLKAFHEVGHALVAKMLPAADTVYKLTIVARGIAGGYTSLLPEEDRTLMSRVQAEAQLAFALGGRTAEEVVFGDVTTGATDDLEKATDMARRMVTQWGMSSKLGPRTYGKKEELVFLGRDISETRNYSEPTALLIDEEISDLIDVAHEKARKIIEKNRDKLDEISEKLLEVETLDSEAFHAMFDGESEASGETESVADEPEEEAPEAESVPEAEEQPKQQPRAVPAPS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be able to modulate cell division in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "PMMPKSWFFDTQSVSCDPENSDIVTLQTPLGMAKFIVIENSGCASLCMMVDVEPLALSSTKEIRFCDTIKVMNNRITAYEC",
    "response#2": "PMMPKSWFFDTQSVSCDPENSDIVTLQTPLGMAKFIVIENSGCASLCMMVDVEPLALSSTKEIRFCDTIKVMNNRITAYEC",
    "response#3": "PMMPKSWFFDTQSVSCDPENSDIVTLQTPLGMAKFIVIENSGCASLCMMVDVEPLALSSTKEIRFCDTIKVMNNRITAYEC"
  },
  {
    "reference": "MILTVTLNPSIDISYCLENFNMDTVNRVTDVSKTPGGKGLNVTRVLSQLGDNVVATGLLGGDFGDFIRSGLDALEIRHQFLSIGGETRHCIAVLHEGQQTEILEKGPHITKDEADAFLNHLKLIFDAATIITVSGSLPKGLPSDYYARLISLANHFNKKVVLDCSGEALRSVLKSSAKPTVIKPNLEELTQLIGKPISYSLDELKSTLQQDLFRGIDWVIVSLGARGAFAKHGNHYYQVTIPKIEVINPVGSGDATVAGIASALEHQLDDTNLLKRANVLGMLNAQETLTGHINLTYYQELISQIQVKEV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein with ATP binding, tagatose-6-phosphate kinase activity is needed to enhance D-tagatose 6-phosphate catabolic process in a specific context.2. The protein should be designed to catalyze the following reaction ATP + D-tagatofuranose 6-phosphate = ADP + D-tagatofuranose   1,6-bisphosphate + H(+).3. Ideal protein should have a binding affinity for key enzymes in the Carbohydrate metabolism pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MKRICVIGSLNMDLVVKVDNMPKAGQTLIGSNFKEVPGGKGANQAVAMARLNGNVSMIGKVGNDGFGQTLINALKNDKVCTNYINVENGPTGVALITVDKNAENCIVVAPGANFKLTPNDIDENIEAIKNSDIVVVQLETPLDTIKYTLKKAKELGKYTILNPAPAVVLEDELIENVDLLTPNETELEIISGVQINCEEDIQLAAKKMIQKGVKELIVTLGSKGSLYINKEKSMFKKAYKVEAIDTTAAGDSYTGALSVAFANNKNIEEAMDFASKVGALSVMKEGAQSSLPL",
    "response#2": "MKKILVIGSFMTDLVFKTSKMPVTGETLIAEKYEKYTGGKGANQAVAASRLGGNVEMIGKLGKDEFGEEHIEKLKEEHIEYQSIMFDDESRTGVGNVTIDKDGNNKILIVPGANLKIVEEEIIKNEHIIKSSDIIVLQLEIPINVVYKCIELAHKYGKTIILNPAPAQEINKNIAHKVDYIIPNETEAEVLTKCKVESVNDAKIAAQILLDQGYKHTIITLGNKGAIYATNGQWKYFNSMNVQAVDTTAAGDSFIGTLAYGIAEGFDIEKIMSIAIKASACTVKKMGAQPSLPTKAELTE",
    "response#3": "MKNILVIGSINMDMVIRVSHIPAVGETILAKDVKSFGGGKGANQAIAAARLDGNVSMIGRVGNDEYGKQSLENLKSSGVNVEGIEIDDNTQTGTAYIYVSDKGENNIVVYAGANEKLSVEQVKRFNYLFDSAEFCVIQLEIPLNVVEFVINLCNKKGVKVIFNPAPACDLSEDVLKNTYILTPNETELAILTGQKTDTISNIESASYKMMERGVENIITTIGDKGSFFVNKGIKQLHEAIKVDSIDTTAAGDSFTGALAVALAEEKEIGEAINFATHVAAITVTKEGAQTSLNKNWLNSFL"
  },
  {
    "reference": "MDKLFDRLQMLEDRYEELGELLSDPDVISDTKRFTKLSKEMADLRETVEKYNKYKEVTQQISDDEEMLSDGLDDEMASLVKEELSNAKNEKVKLEEEIKILLLPKDPNDDKNIIMEIRGAAGGDEASLFAADLFSMYSKYAERQGWSIEVIDKNVTEVGGFKEIALIINGNSVWSKLKYESGAHRVQRIPVTESAGRVHTSTATVVVMPEEEDVEIELDPKDIRVDVYRSSGAGGQHINKTSSAVRMTHLPTGIVVAMQDQRSQQQNREKAMKILKARVYDYYAQQEQSEYDANRKSAVGTGDRSERIRTYNYPQNRVTDHRIGLSLNKLDRIMNGELEDVIDALVLFEQTKAMEKLENE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.2. The designed protein must be optimized for translation release factor activity, codon specific. The designed protein sequence is ",
    "response#1": "QRLGELDFLLSREDIMSDMAQYRTISREHAEITQIAGRYERYKQREADIAGAAEMLEDPDMAEMAREEIAAAQAELVQLEEELQRLLLPKDPDDARNAFLEIRAGTGGDESALFAGDLLRMYTRYCERAGWRCEVVSESESELGGYKEVVIRIAGNDVFGRLRFESGGHRVQRVPATETQGRIHTSACTV",
    "response#2": "IRLEEVSIMLSDPEVVSNVKKFTKLSIEYAQLTPVNQQFQTYLSHLKNLEDAQLILFEDDMEIKTMAKEEILNTKKILSQLDLKLKKSILPKDPNDSRNIIIEIRAGTGGDEASIFSGDLFKIYSRYSEKQKWTIEIISSSIGEHGGFKEIIARISGINVYSKLKFESGAHRVQRVPTTESQGRIHTSACTG",
    "response#3": "QRLGELDFLLSREDIMSDMAQYRTISREHAEITQIAGRYERYKQREADIAGAAEMLEDPDMAEMAREEIAAAQAELVQLEEELQRLLLPKDPDDARNAFLEIRAGTGGDESALFAGDLLRMYTRYCERAGWRCEVVSESESELGGYKEVVIRIAGNDVFGRLRFESGGHRVQRVPATETQGRIHTSACTG"
  },
  {
    "reference": "MIASLRGTVINIGLSSAVIECNGVGYEVVTTPNTLSQLVRGEEALVLTTMVVREDAMKLYGFIDNESREMFSVLQTVSGLGPRLALACESVLSPLEISQAITNADAKTLQRVPGVGKRMADRLIVELKGKVAAFAAGVVDEGGEQISLPNANIASEVVVEQVSQALVGLGFSEKQSDDAVSFVLAADPSLDTSGALRAALAKLSGK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that can perform DNA binding, four-way junction helicase activity, ATP hydrolysis activity, ATP binding in Holliday junction helicase complex to support DNA repair, SOS response.2. For general function, the protein need meet that The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex; HJ branch migration allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.; The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair, while the RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR) The designed protein sequence is ",
    "response#1": "PLEHLLLFGPPGLGKTTLAHVIAHELGVNLRVTSGPAIEKPGDLAAILANSLEEGDILFIDEIHRLSRQAEEHLYPAMEDFVMDIVIGQGPAARTIRLELPRFTLIGATTRPGLITAPLLSRFGIVEHLEYYS",
    "response#2": "TLDHVLLYGPPGLGKTTLASIVANEMGVELRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATTRVGLLTAPLRDRFGVMSRLEYYS",
    "response#3": "LRPKTLDEYIGQERLKQKLRVYLEAAKARKEPLEHLLLFGPPGLGKTTLAHVIAHELGVNLRVTSGPAIEKPGDLAAILANSLEEGDILFIDEIHRLSRQAEEHLYPAMEDFVMDIVIGQGPAARTIRLELPRFTLIGATTRPGLITAPLLSRFGIVEHLNFYTPEELAQGVMRDARLLGVRITEEAALEIGRRSRGTMRVAKRLFRRVRDFAQVAGEEVITRERALEALAALGLD"
  },
  {
    "reference": "MKKIFALALIAPLLISCSSKKNAENAYNEAWIKDTNGFDILMGQFAHNIENIWGFNEVLIAGPKDYVKYTDQYQTRSHINFDEGTITVETIAGTEPAARLRRAIITTLLMGDDPGSIDLYSDVNDIQISREPFLYGQVVDNTGQPIRWEGRASKFADYLLQTHLKSRSNGLRIIYSVTINLVPNHLDKRAHKYIGMVRQASRKYGVDESLILAIMQTESSFNPYAVSHADALGLMQVVQHSAGKDVFRSQGKWGTPSRSYLFDPQSNIDTGTAYLAMLNNVYLGGITNPTSRRYAVITAYNGGAGSVLRVFSSDKDQAVNIINQLSPGDVYETLTNRHPSAESRRYLYKVNTAQKMYRRK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "NMLKNLLTMGGYYATASAKKYYMRTRPFVLFNHSTCRPEDENTLRKNGSYPSGHTAYGTLLALVLSEARPERAQELARRGWEFGQSRVICGAHWQSDVDAGRYVGAVEFARLF",
    "response#2": "GGYYATASAKKYYMRTRPFVLFNHSTCRPEDENTLRKNGSYPSGHTAYGTLLALVLSEARPERAQELARRGWEFGQSRVICGAHWQSDVDAGRYVGAS",
    "response#3": "GQVEKLIYGVFTTPVNSIGGSAVCAFSMKSILESFDGPFKEQETMNSNWLAVPSLKVPEPRPGQCVNDSRTLPDVSVNFVKSHTLMDEAVPAFFTRPILIRISLQYRFTKIAVDQQVRTPDGKAYDVLFIGTDDGKVIKALNSASFDSSDTVDSVVIEELQVLPPGVPVKNLYR"
  },
  {
    "reference": "MVTTLDNALLEEILQQVRPLIGQGKVADYIPALAEVASDRLAIAVCTVDGEIFQAGNATERFSIQSISKVLSLTLALTRYQEHEIWQRVGKEPSGLPFNSLLQLEMEQGKPRNPFINPGALVVCDMLQTRLSAPKQRMLEVVRQLAGEEDLAYDSRVARSEFEHSDRNAAIAYLMKSFGNFENDVLTVLQTYFHYCALRMSCLELARSFVYLANHGRDLSGREVISPLQARQINALMMTSGMYDGAGEFAYRVGMPGKSGVGGGIVAIVPDELSIAVWSPELDASGNSLAGTAALELLSQRISRSIF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have glutaminase activity to facilitate glutamine metabolic process. The designed protein sequence is ",
    "response#1": "PEPPDIIAIGSSTGGPQALFTVLGTMKAGTVRQPIVITQHMPATFTTILAEHIGRVSGWEAREAQDGEAIRGGRVYIAPGDFHMVVETKGTDKVLRLNKNPPENFCRPAVDPMLRSIAAAYGKRVLACILTGMGADGMKGGQAVVASGGTVIAQDEASSVVWGMPGAAATAGICSAVLPLPEIAPWIMKLAARV",
    "response#2": "PEPPDIIAIGSSTGGPQALFTVLGTMKAGTVRQPIVITQHMPATFTTILAEHIGRVSGWEAREAQDGEAIRGGRVYIAPGDFHMVVETKGTDKVLRLNKNPPENFCRPAVDPMLRSIAAAYGKRVLACILTGMGADGMKGGQAVVASGGTVIAQDEASSVVWGMPGAAATAGICSAVLPLPEIAPWIMKLAARS",
    "response#3": "LKDSYKSLVEAIQHAAIANGVKAELDYVDVEAADFKENLAKADALIVPGGFGDRGIEGKIEAVRYARENALPFLGICLGMQCATIEFARNVLGLKKAHSSEFDPRTPHPVVDLMTAQKGVKDKGGTMRLGAWPCSIKAGTLARKIYGSALISERHRHRYEFNSKYIPLFESKGMAVSGTNRKIGLPEMVEIKKHPWFVGVQFHPEFKSKPLLPHPLFFSFS"
  },
  {
    "reference": "MFDNTVSSHKTSIALLKFQKQQQGKRLSLLYNSTITTTTSSSSSRKQVEEEEEEEEEEQEQGQGQDKFQQEQKEEGRDFSIPSSPRTVTTPTTTTSPVPPNITTTVSVTSIVSPRSYNCSNSTCNNNQQLCLSSSSSSSTLSKQQHQQDRINRMSNLLRVAVSKQKRRYQKNGYDLDLAYITDRVIAMGFPSEKVEGVFRNPMKEVQKFLDQYHKDHYRVYNLCSERDYDHNKFYGRVGCYPFDDHNAPAFELIQKFCQDVDEWFKEDPKNIAVVHCKAGKGLMICCWLLYCGMWKNTEESLRFYAALRTYNQKGVTIPSQIRYVHYFGRSMRESIPATKSLLLKKIYLKPLPLDISLQDITFNVSVGKTLVYHSKQVENVTIHRLQKKKIKKSQSKRDLKEKDNTGDKGSSANDATSSTNGSNGVLGSSVRGQSLIDKHHQSLSKGSSQNYKSQIEHTSSNASLSSSSSFDYQAYYLKQQNNAKEEEQEEYISFDVGGIMLAGDIRIEFCNKNERMFMYWFNSTFTSHFESISKTGLDKAHKDKNHKSYPENFRVEMVFDELPVEHNDQNGSVQHQQQQNISTTPLSVAPAIAPPSDPSPSIPITPVKSDNQNGATTVIVNQKDETDSEDSSDSTSSDEHQQQQQEPQQPTNLSSSINVANN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should emphasize Polar residues compositional bias.2. The designed protein must have at least one Phosphatase tensin-type, C2 tensin-type domain.3. The protein should have a low Km value for H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] +   phosphate.4. A protein that can perform phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity, phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity, phosphatidylinositol-3,5-bisphosphate binding, phosphatidylinositol-4,5-bisphosphate binding in cleavage furrow, cytosol, uropod, equatorial cell cortex to support regulation of cell morphogenesis, pseudopodium assembly, uropod retraction. The designed protein sequence is ",
    "response#1": "LDLTYIYPNIIAMGFPAERLEGVYRNNIDEVVRFLDSKHKSHYKIYNLCAERHYDASKFNCQVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDEQHVAAIHCKAGKGRTGVMICAYLLHRGKFQEAQEALDFYGEVRTRDKKGVTIPSQRRYVIYYSFLLTDPQFVVYQLKVKIHTSNPAHTKREDKYMFFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEDNQDKVENGSLVKELDGIQATERGDNDKDYLILVLTKNDLDKANKDKANRLFSPNFKVKLYFP",
    "response#2": "SVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSHLLRRYQEDGFDLDLTYIYPNIIAMGFPAEKLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERNYDAAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVHYYSYLLKNYKPVALLFHKMMFETVPMFTVGTCSESGSGPPLHAHPQFVVYQLKVKIHTSNPAHTRREDKLLLFEFPQPLPVCGDIKVEFFHKQNKVMKKEKMFHFWINTFFIPGPEEASYKLENGSAAPNSDVSDRTYQNPGDANDKDFLVLTLTKNELDKANKDKANKNFSPNFKVRLFFDLTYIYPNIIAMGFPAEKLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERNYDAAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVHYYSYPSQRRYVQYYSNLIPDVQYKVISLYVCEIRFTETNILHNLGTVQCSISDMQDSATEKVKPHRLKTWTIDFQKSCILDISTSPLAVAGDIKVELTQKSSRKMICQFWLNTSFVQKNSACDTDGPTVKYMYTLNKSEIDGAHKDKDHKSFSEDFKISVVFEE",
    "response#3": "YRPVPLLFHKIEFETIPMFSGSTCNPQFVVYQLKVKIFTSTAGPKRAEKLMYFDFPQPLPVCGDIKVEFFHKQNKVMKKEKMFHFWVNTFFIPGPEEYSEKVENGTLVGEQELDGIYSTERSDNDKEYLTLALTKNDLDKANKDKANRLFSPNFKVKLFFTDPQFVVYQLKVKIHTSNPAHTKREDKYMFFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEDNQDKVENGSLVKELDGIQATERGDNDKDYLILVLTKNDLDKANKDKANRLFSPNFKVKLYFK"
  },
  {
    "reference": "MRKETPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGALLLGASSLSGRCWAGSLWLFKDPCAAPNEGFCSAGVQTEAGVADLTWVGERGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVSVLSSGTQAVSGSKDICIKVWDLAQQVVLSSYRAHAAQVTCVAASPHKDSVFLSCSEDNRILLWDTRCPKPASQIGCSAPGYLPTSLAWHPQQSEVFVFGDENGTVSLVDTKSTSCVLSSAVHSQCVTGLVFSPHSVPFLASLSEDCSLAVLDSSLSELFRSQAHRDFVRDATWSPLNHSLLTTVGWDHQVVHHVVPTEPLPAPGPASVTE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that can perform nuclear receptor coactivator activity, methyl-CpG binding in Golgi apparatus, methylosome, nucleoplasm, cytosol, nucleus to support oocyte axis specification, spliceosomal snRNP assembly, histone H4-R3 methylation, negative regulation of epithelial cell proliferation involved in prostate gland development, positive regulation of cell population proliferation.2. For general function, the protein need meet that Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones; Might play a role in transcription regulation; The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.3. The target protein must exhibit strand-strand-strand-strand-strand-helix-helix-strand-strand-turn-strand-strand-strand-strand-strand as its primary conformation. The designed protein sequence is ",
    "response#1": "QPRLSQSLGPKVARRPYFFYGQLGELSPSWWSKISGFLFGIHPEHVDTRLCSALRRTEGYLHNLPTVNRFNTLPNPRLTIQDAMPHMRSWWPQWDIRKHFNSGTCSNMKDATLLCERIGRRIAECKGKPTQQDQTLILRHCHTSNLIWIAPNILSPLEPEHLECIMGYPMNHTNIGGGRLAERLKLFDYCFQTDTLGYHLSVLKSMFPQGLTVLSLFSGIGGAEIALDRLGIHLKGVVSVESCGLSRNILKRWWQTSGQTGELVQIEEIKSLTAKRLETLMQRFGGFDFVICQNPSTPLDLSKEISNSEACEFDYTLFNEFARVTKRVRDMHQKKAFEFLWKNLAGSVVPAMMDPSSDKIGGCVVSHTPGAGKTFLIIAFLASYLKIFPGKRPLVLAPKTTLYTWYKEFIKWEIPVPVHLLHGRRTYCMSKEKTIQFEGIPKPSQDVMHVLDCLDKIQKWHAQPSVLVMGYTSFLTLMREDSKFAHRKYMAKVLRESPGLLVLDEGHNPRSTKSRLRKALMKVDTDLRILLSGTLFQNNFCEYFNTLCLARPKFVHEVLVELDKKFQTNQAEQKAPHLLENRARKFFLDIIAKGWGVRSLDVLHAGAFICEYAGVALTREQANILTMNGDTLVYPARFSSARWEDWGDLSQVLADFERPSYPDIPPVDFAMDVSKMRNVACYISHSTDPNVIVQFVLHDHNSLMFPRVMLFAAENIPPMTELSLDYEYTSFLAPISSSKLYNEVRACREKDRDPEAQFEMPYVVRLHNFHQLSAPKPCFTFSHPNRDPMIDNNRYCTLEFPVEVNTVLHGFAGYFETVLYRDITLSIRPETHSPGMFSWFPIFFPIKQPITVHEGQNICVRFWRCSNSKKVWYEWAVTAPVCSSIHNPTGRSYT",
    "response#2": "QPRLSQSLGPKVARRPYFFYGQLGELSPSWWSKISGFLFGIHPEHVDTRLCSALRRTEGYLHNLPTVNRFNTLPNPRLTIQDAMPHMRSWWPQWDIRKHFNSGTCSNMKDATLLCERIGRRIAECKGKPTQQDQTLILRHCHTSNLIWIAPNILSPLEPEHLECIMGYPMNHTNIGGGRLAERLKLFDYCFQTDTLGYHLSVLKSMFPQGLTVLSLFSGIGGAEIALDRLGIHLKGVVSVESCGLSRNILKRWWQTSGQTGELVQIEEIKSLTAKRLETLMQRFGGFDFVICQNPSTPLDLSKEISNSEACEFDYTLFNEFARVTKRVRDMGWGVRSLDVLHAGAFICEYAGVALTREQANILTMNGDTLVYPARFSSARWEDWGDLSQVLADFERPSYPDIPPVDFAMDVSKMRNVACYISHSTDPNVIVQFVLHDHNSLMFPRVMLFAAENIPPMTELSLDYSVQYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEALYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCSKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPQCLQDFSAGGPDRFYFLEAYNAKSKSFEDPV",
    "response#3": "QPRLSQSLGPKVARRPYFFYGQLGELSPSWWSKISGFLFGIHPEHVDTRLCSALRRTEGYLHNLPTVNRFNTLPNPRLTIQDAMPHMRSWWPQWDIRKHFNSGTCSNMKDATLLCERIGRRIAECKGKPTQQDQTLILRHCHTSNLIWIAPNILSPLEPEHLECIMGYPMNHTNIGGGRLAERLKLFDYCFQTDTLGYHLSVLKSMFPQGLTVLSLFSGIGGAEIALDRLGIHLKGVVSVESCGLSRNILKRWWQTSGQTGELVQIEEIKSLTAKRLETLMQRFGGFDFVICQNPSTPLDLSKEISNSEACEFDYTLFNEFARVTKRVRDMQYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEALYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCSKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPQCLQDFSAGGPDRFYFLEAYNAKSKSFEDPE"
  },
  {
    "reference": "MTIRLNKVTRDLNVGIATVVEFLQKKGYTVEANPNTKITEEQYAMLVKEFSTDKNLRLESERFIQERQNKDRNKASVSIDGYDKKEPEKTVADDVIKTVIPEDVRPKFKPVGKIDLDKLNRKVEKEPVKEEPKPQPVAAEEKKVAEEVKPVVNEVKKEEVTVTPATSEPKPVKEEPKPVVVEKPVETEKKVVEEVKKEEPKVVVSPEKTEKKEEKPVAEAPVTPVEKEEEGVFKIRPTEFVSKINVIGQIDLAALNQSTRPKKKSKEEKRKEREEKEKLRQDQKKQMKEAIIKEIRKEDSKQAKVVGKENLDPNGKKKRNRINNNKEKVDVNNVASNFAHPTPNSERTNNNRGGNQQGGGGQNRNRNNNNKDRFKKPVVKQEVSEEDVAKQVKETLARLTSKGKNKGAKYRKEKRDMASNRMQELEDQEMAESKVLKLTEFVTANELASMMNVSVNQVIGTCMSIGMMVSINQRLDAETINLVAEEFGFKTEYVSAEVAQAIVEEEDAPEDLEHRAPIVTVMGHVDHGKTSLLDYIRKANVIAGEAGGITQHIGAYHVTLEDGRKITFLDTPGHEAFTAMRARGAKVTDIAIIIVAADDDVMPQTKEAINHAAAAGVPIVFAINKIDKPHANPEKIKETLAQMNYLVEEWGGKYQSQDISAKKGLGVPELMEKVLLEAEMLDLKANPNRNATGSIIESTLDKGRGYVATVLVSNGTLKVGDIVLAGTSYGRVKAMFNERNQRVAQAGPSEPVLILGLNGAPAAGDTFH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should emphasize Polar residues, Basic and acidic residues compositional bias.2. For general function, the protein need meet that Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.; One of the essential components for the initiation of protein synthesis; Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits3. A protein that localizes to cytoplasm and can perform GTPase activity, translation initiation factor activity with high specificity and efficiency.4. The presence of tr-type G domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "VVTIMGHVDHGKTSTLDYIRKAHVASGEAGGITQHIGAYHVDTDNGMITFLDTPGHAAFTAMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPLIVAVNKIDKEGANPDNVKNELAQYDVIPEEWGGENIFVHISAKQGTNIDGLLESIPRAPVVTVMGHVDHGKTRLLDAIRNTNVASGEAGGITQHIGAYQVTTTVDGEERKITFIDTPGHEAFTAMRARGAQATDIAVLVVAADDGVKPQTAEAIDHAKAADVPIVVAVNKIDLPTADPQKVRAQLTEYGLVAEEYGGNVQFVDISAKENLNIDQLLEAIILTADAELASGVVIESFLDKGRGPVATVLVREGTLNRGDIVLCGFEYGRVRAMRDEVGRDVASAGPSIPVEILGLSGVPAAGDEATVVRDEKKAREVALYRGA",
    "response#2": "VVTIMGHVDHGKTSLLDYIRRAKVAAGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTAMRARGAKATDIVVLVVAADDGVMPQTIEAIQHAKAGNVPLIVAVNKMDKPEADIDRVKSELSQHGVMSEDWGGDNMFAFVSAKTGAGVDDLLEGIPVVTIMGHVDHGKTSTLDYIRKAHVASGEAGGITQHIGAYHVDTDNGMITFLDTPGHAAFTAMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPLIVAVNKIDKEGANPDNVKNELAQYDVIPEEWGGENIFVHISAKQGTNIDGLLEAAGVVIESQLDKGRGPVATILVQEGTLRQGDIVLCGLEYGKIRAMKDENGRSITEAGPSIPVEILGLSGVPSAGDEATAPVVTVMGHVDHGKTSLLDALRHANVVSGEAGGITQHIGAYQVTAPDSGKKITFIDTPGHAAFTAMRARGAKVTDIVVLVVAADDGVMPQTIEAINHAKAAKVPMIVAINKIDKPDARPDRVRTELLQHEVQVESLGGEVVDVEVSAKNKTNLDRLLEMPRSPVVTVMGHVDHGKTSLLDALRHANVVSGEAGGITQHIGAYQVTAPDSGKKITFIDTPGHAAFTAMRARGAKVTDIVVLVVAADDGVMPQTIEAINHAKAAKVPMIVAINKIDKPDARPDRVRTELLQHEVQVESLGGEVVDVEVSAKNKTNLDRLLEMIALQADILELYKNPERFKAKGTVVEAKLDKGRGPVATLLIQNGTLRVGDSIVVGSTYGRIRAMFNDKGRNIESAGPSTPVEILGLSEVPEAGDKFYQVKEEKTARGIADKRKLQSTHKVSLEDLYNQIQEGTVKELGLIVKADVQGSVEALKQSLEKLSTEEVKVRVIHGGVGAINETDVTLATASNGIILGFNVRPDNNAIIASERDGVDIKTYRVIYDAIEDI",
    "response#3": "VVTIMGHVDHGKTSTLDYIRKAHVASGEAGGITQHIGAYHVDTDNGMITFLDTPGHAAFTAMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPLIVAVNKIDKEGANPDNVKNELAQYDVIPEEWGGENIFVHISAKQGTNIDGLLESIPRSPVVTVMGHVDHGKTSLLDALRHANVVSGEAGGITQHIGAYQVTAPDSGKKITFIDTPGHAAFTAMRARGAKVTDIVVLVVAADDGVMPQTIEAINHAKAAKVPMIVAINKIDKPDARPDRVRTELLQHEVQVESLGGEVVDVEVSAKNKTNLDRLLEMIALQADILPRSPVVTVMGHVDHGKTSLLDALRHANVVSGEAGGITQHIGAYQVTAPDSGKKITFIDTPGHAAFTAMRARGAKVTDIVVLVVAADDGVMPQTIEAINHAKAAKVPMIVAINKIDKPDARPDRVRTELLQHEVQVESLGGEVVDVEVSAKNKTNLDRLLEMIALQADILKRSPIITVMGHVDHGKTSLLDAIRKSKVTSTEAGGITQHIGAYTVELNGEAITFLDTPGHAAFTAMRARGAQVTDIVILVVAADDGIMPQTQEAISHCKAANVPLIVAINKIDRPGANIDKVKQELTEYGLVAEDWGGDTICVPVSAHTKEGIDDLLEMILLSSEILELNPERDLARGTIIEAHLDRARGPVATLLVQNGTLRVGDIIVAGSVMGKIRAMISDRGEKVTDATPSFAVEILGLSEVPAAGDEFEVYSSEKEARAIADERAERWLS"
  },
  {
    "reference": "MTATAPREAQLWSRCAGCATLLYRKRLHRNLDVCPECGTHARLGAPDRLRQLVDPGSFTMLPDRPAEVDPIGFVDVLPYPHRLTAARAGTGLAEAVLCGTATIGRHPCALAVMDFRFLGGSLGSAVGELITRAAERALADRIPLVLVTASGGARMQEGALSLMQMATVSQAVAALREAGLLTVSVLTDPTYGGVAASFATNTDVVIAESGARMGFAGPRVIRQVTGRALPDGFQTADFLLRHGQVDMVVPRRALRSRLTALLAAARAARRPDPAPDVRPSQPAAVDPATASSASAAGGPAGDAWATVRAARHPDRPTTLDYLESAFDDFVELHGDRLGGDCPAVVGGLARLAGHPVMVIGHQKGHTTAELVARNFGMASPAGHRKALRLMRLAARLGLPVVTLVDTPGADPGVAAEQQGQAAAIAENILALTMLPTPVVAVITGEGGSGGALALAVADRVLMLRHAVYSVISPEGCAAILWPDRGAAPQAARALRLTAPDLCRLGVVDEVVPEPDPAAQDDPEAAGRLLRVALLANLTPLLEVAPATLVRRRRQRFRRFGAAGVRAGAR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Component of the acetyl coenzyme A carboxylase (ACC) complex2. The presence of CoA carboxyltransferase N-terminal domains is critical for the interaction of the designed protein with its intended targets.3. The designed protein should be optimized for carboxyl- or carbamoyltransferase activity, zinc ion binding activity in order to improve malonyl-CoA biosynthetic process, fatty acid biosynthetic process. The designed protein sequence is ",
    "response#1": "MTDVSRVLKEARDQGRLTTLDYANLIFDDFMELHGDRHFSDDGAIVGGLAYLAGQPVTVIGIQKGKNLQDNLARNFGQPNPEGYRKALRLMKQAEKFGRPVVTFINTAGAYPGVGAEERGQGEAIAKNLMEMSDLKVPIIAIIIGEGGSGGALALAVADQVWMLENTMYAVLSPEGFASILWKDGSRATEAAELMKITAGELYKMGIVDRIIPEHGYFSSEIVDIIKANLIEQITTT",
    "response#2": "MTDVSRVLKEARDQGRLTTLDYANLIFDDFMELHGDRHFSDDGAIVGGLAYLAGQPVTVIGIQKGKNLQDNLARNFGQPNPEGYRKALRLMKQAEKFGRPVVTFINTAGAYPGVGAEERGQGEAIAKNLMEMSDLKVPIIAIIIGEGGSGGALALAVADQVWMLENTMYAVLSPEGFASILWKDGSRATEAAELMKITAGELYKMGIVDRIIPEHGYFSSEIVDIIKANLIEQITR",
    "response#3": "LWVKCPDTGQMVFHKEVEQNHWVIPGSEHHLKMSATARLKMMFDEGTWIDVPLPEVPADPLKFRDEKRYVDRLKEARAKTGMPDAFKIGFGRVGSLPMTIAAQEFGFMAGSLGMAGGEAFVRGAETALEKRTPYVLFAASGGARMQEGILSLMQMPRTTVAVRRLRAARLPYIVVLTNPTTGGVTASYAMLGDVHLAEPGALICFAGPRVIEQTIREKLPDGFQRAEYLREHGMVDQVVHRHQLKETISRLCGLLMDVRRTPQPGTAPEH"
  },
  {
    "reference": "MSSLLPVAVYGLEVPPGDILVPAQFEFPATIRITMAAIDPTAEPETDGQGNVPTVPRSTLKLIKTTAEEDDDEYLDIDGEDSEDDEESDDEEVNGGPSDPAKSKKARREAAIKKLLEATKEESDEEMEDADAKPNGKGKKDSKGKAKASESDDEKSDEDDEEGEPNFEEFVVCTLDTERTYQQPIDITITEGEKVFFVVKGTHKVYLTGNYVLPEGQDEEDDEDEEDYSDEEYDLPHGIELESDSDYESDELDEIDGTPRIKEIDTDEEEEEAPKLVDTSKKGNKKRPAEEAENLDDLVAKDKKQAEKQKKLKNNKGEAVPAENKDVKKEGKSDKKVQFAKDLEQGPSGPAKDKLEKKEEKKDDKADLKKPSLGVKVVQGVTIDDRKLGTGRTVKSGDRVSLRYIGKLTNGKVFDANKKGAPFTVRVGKGEVIKGWEIGLIGMQVGGERRLTIPPHLAYGSRAMPGIPANSTLVFDIKLLEIK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that localizes to nucleus and can perform peptidyl-prolyl cis-trans isomerase activity with high specificity and efficiency.2. The designed protein must have at least one PPIase FKBP-type domain.3. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.4. The protein must exhibit the following characteristics: Histone proline isomerase that increases the rate of cis-trans isomerization at prolines on the histone H3 N-terminal tail. The designed protein sequence is ",
    "response#1": "LEDFRADIRANLERELKGALVSHLRREIGEQLIAAYAHVEMPPRLVEKEARLMLAKQIEQIRLSGRDPTVIPDDAHIGFMDAACKRVLVGLLVGEIAGRNRLRLDPMRVTETLHLIASTYEEPEEVFQMYRNDPKLMEGVQSLVMEEQVIEWIR",
    "response#2": "LEDFRADIRANLERELKGALVSHLRREIGEQLIAAYAHVEMPPRLVEKEARLMLAKQIEQIRLSGRDPTVIPDDAHIGFMDAACKRVLVGLLVGEIAGRNRLRLDPMRVTETLHLIASTYEEPEEVFQMYRNDPKLMEGVQSLVMEEQVIEWIR",
    "response#3": "LEDFRADIRANLERELKGALVSHLRREIGEQLIAAYAHVEMPPRLVEKEARLMLAKQIEQIRLSGRDPTVIPDDAHIGFMDAACKRVLVGLLVGEIAGRNRLRLDPMRVTETLHLIASTYEEPEEVFQMYRNDPKLMEGVQSLVMEEQVIEWIR"
  },
  {
    "reference": "SADIFLGVPFNIASYALLTHLVAKECGLEVGEFIHTFGDAHIYSNHMDAIHTQLSRDSYLPPQLKINTDKSIFDINYEDLELINYESHPAIKAPIAV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be able to bind dUMP ligand in a variety of conditions2. The protein design should take into account the physiological conditions of the dTTP biosynthesis pathway.3. The designed protein must be able to regulate dTMP biosynthetic process.4. We are looking for a protein with broad substrate specificity for (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-   dihydrofolate + dTMP. The designed protein sequence is ",
    "response#1": "GLGNEGGLPWKCNSVDMKHFSSVTSYVNETNYMRLKWKRDRYMEKNNVKLNTDGIPSVDKLQNIVVMGKASWESIPSKFKPLQNRINIILSRTLKKEDLAKEYNNVIIINSVDDLFPILKCIKYYKCFIIGGASVYKEFLDRNLIKKIYFTRINNAYTCDVLFPDINEDLFKITSISDVYSSNNTTLDFVIR",
    "response#2": "GLGNEGGLPWKCNSVDMKHFSSVTSYVNETNYMRLKWKRDRYMEKNNVKLNTDGIPSVDKLQNIVVMGKASWESIPSKFKPLQNRINIILSRTLKKEDLAKEYNNVIIINSVDDLFPILKCIKYYKCFIIGGASVYKEFLDRNLIKKIYFTRINNAYTCDVLFPDINEDLFKITSISDVYSSNNTTLDFVIKA",
    "response#3": "GLGNEGGLPWKCNSVDMKHFSSVTSYVNETNYMRLKWKRDRYMEKNNVKLNTDGIPSVDKLQNIVVMGKASWESIPSKFKPLQNRINIILSRTLKKEDLAKEYNNVIIINSVDDLFPILKCIKYYKCFIIGGASVYKEFLDRNLIKKIYFTRINNAYTCDVLFPDINEDLFKITSISDVYSSNNTTLDFVIQ"
  },
  {
    "reference": "MYSTLNPLVDPATVTDLASRLHSVLNDTRELVIDSRRANAATIFCAYPGATLDGRNFIAQAIQNGCDCILWEKGIDFTYSVENYSFKNLQHYTGLIAASKAHFPSQKFYSIAVTGTNGKTSISHWLNQAYSYLGYKSAIIGTTGAGIYPNVTDYASTTPDPITLQHLLHDFSRQDVQYLAMEVSSHALSQGRVNGVDFKCAIFTNLTQDHLDYHKTMADYFAAKKQLFYWRSLEHAVINTDDLYGAELLNDLRENNPRLNLISYGIESGDLRADNLHLSSSGISFDLLYQGMKQEIKVAVIGRFNVYNLLAVFATLLVKQIAWEKLAEIARQLKPVTGRMDAVIIPNKPLVVVDYAHTPDALEKALSTLQEIKHTGKLFCVFGCGGNRDRGKRPQMGRIAAELADEVIITTDNPRDEEPLQIIRDITVNLTSSNYQLIVDRAEAIRAALAAAKTGDIVLVAGKGHETYQEIHGVKHHFSDFELVNEILQIN",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that can perform UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity in cytoplasm to support cell cycle, cell wall organization, cell division.2. The protein should have a low Km value for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.3. The designed protein must have at least one Mur ligase central domain.4. A protein that can modulate the overall flux of metabolites through the peptidoglycan biosynthesis pathway, enhancing or suppressing its activity as needed.5. The ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MRLSELLKGIVDYQVVGDGDVEVTGISLSTQRIHPGDLYAALPGTRAHGASYSAAAVEAGAAAIVTDAAGAEIVAGLDLDVPVLVVPDPRAVLGTLSAHVYQQPSDKLILVGITGTNGKTTVSHLVEQIVRENGYKVGLIGTIEAIIGNEKIHSKNTTPESYLLQQYFSDMAAAGVDYCVMEVSSHALMQNRVAECEFDVGVFTNLTQDHLDFHKTFDEYFAAKAKLFTMCKTAVINLDDAYGKRLISQIAPEKVTTFGINNKADLQATDLNIQPQGMSFQVKTAAGNIDIESKLIGEFNVYNILSAIGAALSLGFDLETIKKGLAEVPQVPGRLETFESAGLTVIVDYAHTPDALDKALQGVRQHCSGKLVCVFGCGGDRDRGKRPEMGAIAASRADKLLVSSDNPRNENPEQIIDDILAGIDANQCELLVEVDRRMAIERAIHEAAAGDCVLIAGKGHEDYQIIGDKKYHFDERQIVSEYLKQQ",
    "response#2": "MKLKDLIGKSSEILLKGNTDIEISHIAQDSRKVKPGTLFICIDGELVDGHQFASRAVELGAVAIIAEKQLDVSIPVIYVRDSKRAMAMLANHFYENPSKKLKLIGVTGTNGKTTTSHLLAAIGRATGLKVGLIGTIANRIGDRVLPVEHTTPESLDLQQLLAEMVAEKVALAVMEVSSHALALNRVAGCDFDLGIFTNITQDHLDFHHDMDDYLAAKSRLFASLGAADGGRPGTAVINRDDPRAERIINVTRAAVTTYGMHGPADVRAEKIQVTPRGVHFHVTSPWGRGEVRLKLTGLFNVYNALAALATGVALNIPFNIIKDGIESYKNVPGRLEQVETGRDFHVVVDYAHKPDAVEAALSTLRPLTAQAGGRLICVIGAGGDRDTGKRPVMGEIAARLADLVIVTDDNPRSEDPAVIRAAVLEGTRDGAGEVREVGDRREAIRVALSSAGPGDIVLVAGKGHENYQIFGKEIIPFDDAKEAKKILEEMK",
    "response#3": "MKLQDLLSRVDGVRLQGSAGDHVVADSRRVQAGDVFLAFQGEYADGRQHIQAAIDAGAAAVLWEAEDFPDQLIPDNAALVGITGTNGKTTVSHLVEQIVRENGRRAAMIGTLGVRVGDREIPAPNTTPEAVVLQRLFAEMADAGADFAAMEVSSHALAQGRVDGCRFRAAVFTNLSQDHLDFHKTMDAYFAAKAKLFTPEYLAENADAVLNADDEYGQRIIARLPAGRVLTYSLKGAADVRATDFTLDKHGTRFTLHTPAGTAELRSPLIGAHNVENILAAAAAGLVLGIPLADIAAGIEAVPQVPGRLEQVETGRDFHVVVDYAHKPDAVEAALSTLRPLTAQAGGRLICVIGAGGDRDTGKRPVMGEIAARLADLVIVTDDNPRSEDPAVIRAAVLEGTRDGAGEVREVGDRREAIRVALSSAGPGDIVLVAGKGHEDYQIIGDRTIHFDDREVARAALAERAG"
  },
  {
    "reference": "MTEHEAIVKQLQAVQNASRKIVPLNEETINGLLVELADRIPAAADAILEANRKDLERMDPADPRYDRLLLNEARLNSIATDLRNVAALPSPLHRVLEERTLPNGLELKKVSVPLGVIGIIYESRPNVTFDVFALCLKSGNATVLKGGSDAAYSNIAIVNLIQTVIRDRGLDPDMIYLLPAEREAAHILLNAVGYIDVIIPRGSQALIDFARKHSTVPVIETGAGIVHTYFDQSGDLAMGRDIVFNAKTRRPSVCNALDTLIVHESRIDDLPVLVVPLEEKNVQIFADEPAYYKLLGRYPDELLEMASPEHFGTEFLSLKMSIKTVGSLEEALNHIARHSSKHSEAIIASDQTTIDAFMKQVDAAAVYANTSTAFTDGAQFGLGAEIGISTQKLHARGPMALKELCSYKWLVTGQGQVRTA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Catalyzes the NADPH-dependent reduction of L-glutamate 5- phosphate into L-glutamate 5-semialdehyde and phosphate; The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.2. The protein design should be able to enhance the efficiency of L-proline biosynthetic process.3. The protein design should consider the endogenous proteins and enzymes present in the Amino-acid biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MGKAAKAASWQLAVLTSAQKDSALQVIADSLEANSQSILDANAQDMAEARRNGLVDALLDRLLLNPARLSAIAADVRQVCRLTDPVGQVIDGQRLDSGLSLERRRVPLGVVGVIYEARPNVTVDVAALCLKTGNAVILRGGKETYRTNAATVKVIQQALTDCGLPACAVQAIEDPDRALVNALLRLDRYVDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHIYVDDTIDFEQALPVIENAKIQRPSACNSLETLLVHQAIAERFLPRLSHHMHTLGVTLHAR",
    "response#2": "MGKAAKAASWQLAVLTSAQKDSALQVIADSLEANSQSILDANAQDMAEARRNGLVDALLDRLLLNPARLSAIAADVRQVCRLTDPVGQVIDGQRLDSGLSLERRRVPLGVVGVIYEARPNVTVDVAALCLKTGNAVILRGGKETYRTNAATVKVIQQALTDCGLPACAVQAIEDPDRALVNALLRLDRYVDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHIYVDDTIDFEQALPVIENAKIQRPSACNSLETLLVHQAIAERFLPRLSHHMHTLGVTLHAVPSLDAAIAHINEYGSGHTDAQQLGHNAKQASYALANLTASQKADLLDAIADALTENTLAILAANAKDVAAAKAEGLNDAMIDRLLLNESRLAGIIGDISDVVRLADPVGEEFGSRVLDNGLRLTRRRVPLGVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLESNKLISEVIRGAIASQGLPVDAVQLIDSSDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKAANLERALEVIANAKVQRPTVCNALDTLLVDKTIAANFVPQIVEYLHCLGVRFSVCEQSHALLDGLGFDIELATEQSFATEWLSLTLGIKVVSDIDAAIAHIRRHSSGHSEAILTDDIHAATHFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKWLAWGQ",
    "response#3": "MGKAAKAASWQLAVLTSAQKDSALQVIADSLEANSQSILDANAQDMAEARRNGLVDALLDRLLLNPARLSAIAADVRQVCRLTDPVGQVIDGQRLDSGLSLERRRVPLGVVGVIYEARPNVTVDVAALCLKTGNAVILRGGKETYRTNAATVKVIQQALTDCGLPACAVQAIEDPDRALVNALLRLDRYVDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHIYVDDTIDFEQALPVIENAKIQRPSACNSLETLLVHQAIAERFLPRLSHHMHTLGVTLHAVPSLDAAIAHINEYGSGHTDASAAEKIDTAVKNHALIAMARAIRCHEASLLAANAADVAQARNKGLEPAMIDRLTLTPKGIASMAAGLEQIAALSDPIGAVTDLDYRPSGIQVGRMRVPLGVIAIIYEARPNVTADAAGLCLKAGNAAILRGGSEAIQSNQAIAACVQEGLRSAGLPEHAVQVVETTDRAAVGELITMSEYVDMVVPRGGKGLIERIANEARVPVIKHLDGVCHVYVDLSADLEKAVRVADNAKTQRYGTCNTMETLLVHAGIAERFLPRICKILLEKGVELRGDEAARALVAGIKPAVEEDWYAEYLAPVLSVRIVEDIDQAITHIATYGSQHTDAIVTEDYSRARQFLREVDSSSVMINASTRFADGFEYGLGAEIGISTDKLHARGPVGLEGLTSQKFIVLGA"
  },
  {
    "reference": "MGWLDFNSNSKKEKGKDDARSSFSWGDNLNATDWQHYTDPRTLIPTLLLTTTILFSTRLYRSYLRRIPEATHIRPGFFRKRSLFGTVTRVGDADNFHLFHTPGGRLAGWGWLPGRKTLPEGKDLKNKTIHVRIAGVDAPEGAHFGKPAQPFSAEALAWLRDYIQNRRVRAYIYRRDQYNRVVATVWVRRFLFRKDVGKEMLKAGMATVYEAKMGAEFGDFEAQYRAIEKEAKKNKLGMWSGKKKDYESPRDYKTRTAAAANILK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein can be expressed in membrane, cytoplasm, mitochondrion and perform metal ion binding, endonuclease activity without triggering undesirable cellular responses.2. The designed protein should have a high specificity for Ca(2+) binding, with minimal off-target effects.3. The protein should have a Helical domain that is long enough to span the entire membrane.4. The designed protein have TNase-like domains with specific sequence motifs that are crucial for its activity.5. The designed protein must have a active site that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "QHQFVSGYSRDFLMPLWTSYTVDRNDSFSAENFSNCLYQDLRISLSPVHKCSFYKNNAELTYGFLYPPQLNKGSNQLYSEALLNTNIVPMYQSFQVIWRYFHGTLLPRYAEERNGVNVVSGPVFDSDYDGCYDSSETLKQNSRLIRNQEILIPTV",
    "response#2": "QHQFVSGYSRDFLMPLWTSYTVDRNDSFSAENFSNCLYQDLRISLSPVHKCSFYKNNAELTYGFLYPPQLNKGSNQLYSEALLNTNIVPMYQSFQVIWRYFHGTLLPRYAEERNGVNVVSGPVFDSDYDGCYDSSETLKQNSRLIRNQEILIPTR",
    "response#3": "QHQFVSGYSRDFLMPLWTSYTVDRNDSFSAENFSNCLYQDLRISLSPVHKCSFYKNNAELTYGFLYPPQLNKGSNQLYSEALLNTNIVPMYQSFQVIWRYFHGTLLPRYAEERNGVNVVSGPVFDSDYDGCYDSSETLKQNSRLIRNQEILIPTC"
  },
  {
    "reference": "MFSRDVRLETYDPELAKAIAAEAGRQEDHVELIASENYCSPLVMEAQGSQLTNKYAEGYPGKRYYGGCEFVDIAEQLAIDRIKQVFGADYANVQPHSGSQANQAVYLALLQPGDTILGMSLAHGGHLTHGAKVNASGKLFNAVQYGVNEQGLIDYDEVQRLATEHKPKMVIAGFSAYSQKIDWARFRAIADSVGAYLFVDMAHVAGLVAAGVYPSPMDHAHVVTSTTHKTLRGPRGGIILAKGAGEDLVKKLQSIVFPGIQGGPLMHVIAAKAVAFKEALEPEFKTYQQQVVKNAQAMANTLIARGYKIVSGGTENHLMLVDMIGRDVSGKDAEAALGKAHITVNKNSVPNDPRSPFVTSGLRLGTPAITTRGYQEQDCVDLANWIADVLDAPADDAVLAKVRDAVTAQCKKYPVYG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. pyridoxal 5'-phosphate is required for the proper folding and stability of the protein.2. The protein should have a high affinity for (6S)-5,6,7,8-tetrahydrofolate.3. The protein design should consider the endogenous proteins and enzymes present in the glycine from L- serine: step 1/1, glycine biosynthesis, Amino-acid biosynthesis pathway to avoid interference with native metabolic processes.4. For general function, the protein need meet that Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.; This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier5. A protein that can perform zinc ion binding, glycine hydroxymethyltransferase activity, pyridoxal phosphate binding, serine binding in cytosol, cytoplasm to support one-carbon metabolic process, glycine biosynthetic process from serine, tetrahydrofolate metabolic process, folic acid metabolic process, L-serine catabolic process. The designed protein sequence is ",
    "response#1": "LADTDPEIFGAIGKELGRQRHEIELIASENIVSRAVLEAQGSIMTNKYAEGYPGKRYYGGCQYVDIAEELAIERAKKLFGVNFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHLTHGSPVNMSGKWFNVVSYGVREGDNLLDMEAVQRKAEETKPKLIIAGGTAYSRIWDWKRFREIADSVGAYLMVDMAHIAGLVAGGQHPSPFPHCHVATTTTHKSLRGPRGGMILTNDEDLAKKFNSAVFPGLQGGPLMHVIAAKAVALGEALQPEFQDYAAQIVKNAKALSETLISGGVDVVSGGTDNHLMLVDLRKKNATGKRAEAALGRAYVTCNKNGIPFDPEKPFVTSGVRLGTPAGTTRGFKEAEFREIGNLIADTLDNIDDNELIKESLNKELKRQQSHIELIASENYVSKAVLELNGSVLTNKYAEGYPGKRYYGGCEFIDEIESLGIQTAKELFHAEHANIQPHSGSQANDAAYKALLEPKDRVVAMSLDAGGHLTHGYPINFSGYTYDFRFYGVNKDTEQLDYQEIEKIVLEHKPKLIVAGASAYSRIIDFKKFKEIADKVGAYLMVDMAHIAGLVAAGVHPNPLEYADIVTTTTHKTLRGARGGLILCKQEFAKKVDLAVFPGSQGGPLENLIAGKTQALLEASTDEFKEYGKQIVKNTKALANVLQENGLRLVAGGSDNHLINVDVKSTLRITGKKAEKILESIGIICNKNMIPFDTEKPFYTSGIRLGTPAMTTRGFKEEEFKQVGLLADTDPEIFGAIGKELGRQRHEIELIASENIVSRAVLEAQGSIMTNKYAEGYPGKRYYGGCQYVDIAEELAIERAKKLFGVNFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHLTHGSPVNMSGKWFNVVSYGVREGDNLLDMEAVQRKAEETKPKLIIAGGTAYSRIWDWKRFREIADSVGAYLMVDMAHIAGLVAGGQHPSPFPHCHVATTTTHKSLRGPRGGMILTNDEDLAKKFNSAVFPGLQGGPLMHVIAAKAVALGEALQPEFQDYAAQIVKNAKALSETLISGGVDVVSGGTDNHLMLVDLRKKNATGKRAEAALGRAYVTCNKNGIPFDPEKPFVTSGVRLGTPAGTTRGFKEAEFREIGNL",
    "response#2": "LAKTDPDLWAAIQQEDRRQQDHIELIASENYTSPAVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKALFGAEAANVQPNSGSQANQAVFMAFLKPGDTIMGMSLAEGGHLTHGMALNMSGKWFNVVAYGLNEKEEIDYEAMERLAREHKPKLIIAGASAYALRIDFERFAKIAKEIGAIFMVDMAHYAGLIAAGLYPNPVPHADVVTSTTHKTLRGPRGGIILMKAEHEKAINSAIFPGLQGGPLMHVIAGKATAFKEAATKDFKRYQEQVIDNALVMCKVLVERGLRIISGRTESHVFLVDLRAKNLTGKEAEALLGRAHITVNKNAIPNDPQKPFVTSGIRIGTPAMTTRGFTELEAEQLAHLIADVLDGLDDPAAREADPAVADALEGERGRQNDTLAMIASENHVSEAVMEAQSSELTNKYAEGYPGERYYGGCEYADDVEELAIDRAKELWGADHVNVQPHSGSQANMGVYLGVLEPGDKILSLDLTHGGHLSHGHPANFAGQVYEVEQYKVDEETGYVDYEGLHDHAEEFEPDIIVSGYSAYPREVDFERIQEAADAVDAYHLADIAHITGLVAAGVHESPVGVADFVTGSTHKTIRAGRGGIIMCDEEYADDIDAAVFPGSQGGPLMHNVAGKAVGFGEALAPEFEQYAQQTVDNAIALGEQFKEHGLSLVSGGTDNHLVLIDLRPSHPDTTGKEVEEALEEAGIVLNANTVPGETRSAFNPSGIRAGTPALTTRGFDEDACREVADLIADVLGGLDDPAARERVREADPAVADALEGERGRQNDTLAMIASENHVSEAVMEAQSSELTNKYAEGYPGERYYGGCEYADDVEELAIDRAKELWGADHVNVQPHSGSQANMGVYLGVLEPGDKILSLDLTHGGHLSHGHPANFAGQVYEVEQYKVDEETGYVDYEGLHDHAEEFEPDIIVSGYSAYPREVDFERIQEAADAVDAYHLADIAHITGLVAAGVHESPVGVADFVTGSTHKTIRAGRGGIIMCDEEYADDIDAAVFPGSQGGPLMHNVAGKAVGFGEALAPEFEQYAQQTVDNAIALGEQFKEHGLSLVSGGTDNHLVLIDLRPSHPDTTGKEVEEALEEAGIVLNANTVPGETRSAFNPSGIRAGTPALTTRGFDEDACREVADL",
    "response#3": "LSQFDPAVAEAIQHETERQEYNLELIASENFVSEAVLEAQGSVMTNKYAEGYPGKRYYGGCHHVDVVENLAIERAKELFGAEHANVQPHAGSQANMAVYNAVCQPGDTILGMNLSHGGHLTHGSPVNFSGRFYNVVPYGVSPDTETIDYNEVERLALEHKPKMIVVGASAYPRIIDFPAFRAIADKVGAKVMVDMAHIAGLVAAGVHPNPVPYAEFVTTTTHKTLRGPRGGMILCREEYAKTINSQIFPGIQGGPLMHVIAAKAVAFKEALQPEFKTYQQQIVKNAAKLAECLMAKGFKLTSGGTDNHLMLINFTGTEITGKAAEEALDKAGITVNKNTVPFETRSPFVTSGIRVGTPACTSHGLKETEMEQVAGFICDVLDNIQDEENLNKVKSEVLELTSKFPVYG"
  },
  {
    "reference": "MQKQKTLAASFSLKGKGLHTGLDIEITFNPAPENHGYKIKRVDMEGQPTIDALAENVVNTQRGTVLSKNGVQVSTIEHAMAALYAYEIDNCLIEVNAPEFPILDGSSRFFSEEIQKTGVVEQNAPKDYYIVKHKIEVKDEETGSSLIILPDDKFSVNVLISFDSPVLSNQFATLNDLSEFPTELAASRTFVFVREVEMLLNHNLIKGGDLDNAIVIYDQKIPQESLDKLADMLNIPHKNVQELGYINNKPLVFDNEPARHKLIDVIGDIALIGKPIRGRVIATRPGHKINNQLARMIRKDIKMNEVQAPVYDPNREPVMDINRIRELLPHRYPFLLVDKIIEVGGNYIVGVKNITTNEPFFQGHFPQEPVMPGVLQVEAMAQTGGLLVLNSVDEPERYSTYFMKIDGVKFRQKVVPGDTLILRLELLAPIRRGISTMKGYVFVGDKLVSEAEFMAQIVKNK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein should be able to manipulate the activity of enzymes in the Glycolipid biosynthesis, lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 2/6, lipid IV(A) biosynthesis pathway, either by activating or inhibiting their function.2. A key design requirement for the protein is the optimization of the active site, Proton donor to achieve high catalytic efficiency for a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine +   H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate.3. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects.4. The presence of Zn(2+) is necessary for the protein to function in the desired environment.5. For general function, the protein need meet that Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs. The designed protein sequence is ",
    "response#1": "MSVSGKPESIRPIVLSDLATGNITGQDGDISVTDVTHDSRRAGPGSLFVAVRGERFDAHDFLPQAAASGAVAVVGERQVSLPSPWVVVPDTRVALGPIAAAWYGHPSRQMRMIGVTGTNGKTSVTHFLAQALTLLGRPCGLIGTIENRVGAEVIPSRTTPEATDLQRLLAQMRDAGAVGVAMEVSSHALAQDRLDGCRFDVAVFTNLTQDHLDFHKTMDAYFAAKRRLFTEHLGQDVPARAVINADDPVGAALAAETRAPVITYSLGAPADVRGTVTDVVLTTSGSRFVVDTPAGSASVESPLVGRFNVYNALAAAAACIAQGVAIADIAEGLAAATVVPA",
    "response#2": "MSKKYKLSQIAEEAGVSLKQGNSDRNLDVISISHDSRLVEPGSLFICKGAAFKSDFLAEAVSKGAAAVVSEQELAWNDQPVVLVDDVRKAMSLMAAAFYQQPSAKLKVWGF",
    "response#3": "MSESKQQTLSDLGLTARGGREAKVXAXAXTXDXNXAXRXGEXRXEXTAXVXVTADVRVXGAAXDFAXADXRXAXGXDAXGXGXGXDXGXVXTMNXAXDXXQVLX"
  },
  {
    "reference": "MGRGRVEMKRIENKINRQVTFSKRRAGLLKKAHEISILCDAEVSLIVFSHKGKLFEYSSESCMEKVLERYERYSYAEKQLKAPDSHVNAQTNWSMEYSRLKAKIELLERNQRHYLGEDLESISIKELQNLEQQLDTSLKHIRSRKNQLMHESLNHLQRKEKEILEENSMLTKQIKERESILRTHQNQSEQQNRSHHVAPQPQPQLNPYMISHQASPFLSMGGMYQGEDPTAVRRNRLDLTLEPIYNCNLGYFAA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein have MADS-box domains with specific sequence motifs that are crucial for its activity.2. For general function, the protein need meet that Seems to be partially redundant to the function of APETALA1.; Probable transcription factor that promotes early floral meristem identity in synergy with APETALA1, FRUITFULL and LEAFY; Is required subsequently for the transition of an inflorescence meristem into a floral meristem3. The protein design must be focused on enhancing the efficiency of cell differentiation.4. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "QGFSQLIFGSKYLHSVQEILSHFAAYSLDYSSRGTESGAASSAFTSRFENITEFLDGDSNNSEAGFGSTFQRRALEAKKTHLLDLLQMVDDRYSHCVDEIHTVISAFHAATELDPQLHTRFALQTVSFLYKNLRERICG",
    "response#2": "PKPLSIEPHFSTASKEDDSSLETIVKCKEAEAKLFQKLADDARKEVDSYRQIVRSRTQKLEEEYAAKLAKVCFQETEEKRRKKLEELKMLENSHYDYLKMKMRMQTDIQGLL",
    "response#3": "PKPLSIEPHFSTASKEDDSSLETIVKCKEAEAKLFQKLADDARKEVDSYRQIVRSRTQKLEEEYAAKLAKVCFQETEEKRRKKLEELKMLENSHYDYLKMKMRMQTDIQGLS"
  },
  {
    "reference": "MRPFCYREGKLFCENVFLKDIADKVKTPFYVYSYNGIIYNFNRMKNALSRFNPLICYSLKANSSIALCKILSSLGAGADVVSGGELYLALCAGFSPQKIVYAGVGKTSEEIEYALEKNILLFNVESEEELEEIATISSRLGKKANIALRVNPDVDPETHDYITTGKIENKFGIDLEKARKLFEKIKKIKSLSPRGIHMHIGSQIKSIQPYIKAIQKVKEFIEEIQKKIDIKLDFFDIGGGFGIAYREEEKDFPLEEFADKITHILPKNMRLIVEPGRYIVGNSGVLLTKVLYRKKGREKYFIVVDAGMNDLIRPSLYGACHRVLPVEKESPHNILRKVSIVGPVCESGDFFVKDFDFPEIKKEKLIAILDAGAYGFSMSSNYNARPRCSEVLVKEEKWWVIREREEYEDLLKNQKFPDFIIPFWKMEATGNDFIVIDNRDRVISDRKEAAIRLCNRKKGIGADGLILIEEAEGADFFMRIFNPDGSEAEMCGNGARCAARFAYLKGIAGKICHFKTLSGIIEAKIDGEDVKIKMTDPSKFRKISGIKIDEENHSEFEGYYINTGVPHFVIFSSNLESISVKRIGSKIRFNRIFHPLGTNVDFVETENKRIKIRTYERGVEDETLSCGTGAAASAIVASIVKNLSPPISVLTRGGEVKVWFQKKEGKIYNVFLEGKANKVYKGYLNGGKNV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have a binding site that is specific to the target pyridoxal 5'-phosphate ligand. The designed protein sequence is ",
    "response#1": "MDFEAFFTTELQSLHSEGRYRVFADIERQQGNFPRATRYNANGQRKDVTVWCSNDYLGMGQNPKVIEAMKAAIDHCGAGAGGTRNISGTNHYHVLLEQELADLHGKESALIFTSGYVSNWATLGTLGQKIPGLIIFSDALNHASMIEGIRYGRCERVIWKHNDLEDLEAKLKAADPNAPKLIAFESVYSMDGDIAPIKEICDLADRYGAMTYLDEVHAVGMYGPRGGGIAEREGLMDRLTIIEGTLGKAFGVMGGYITGSTAVCDFIRSFASGFIFTTALPPSLAAGAIASIQHLKASPFERARHQDRVRKLRGLLDARGIPHMDNPSHIVPVMVGDAAKCKWISDILLDSHGVYVQPINYPTVPRKTERLRITPTPLHSDADIEHLVGALHQLWSHCALARL",
    "response#2": "KDVTVWCSNDYLGMGQNPKVIEAMKAAIDHCGAGAGGTRNISGTNHYHVLLEQELADLHGKESALIFTSGYVSNWATLGTLGQKIPGLIIFSDALNHASMIEGIRYGRCERVIWKHNDLEDLEAKLKAADPNAPKLIAFESVYSMDGDIAPIKEICDLADRYGAMTYLDEVHAVGMYGPRGGGIAEREGLMDRLTIIEGTLGKAFGVMGGYITGSTAVCDFIRSFASGFIFTTALPPSLAAGAIASIQHLKASPFERARHQDRVRKLRGLLDARGIPHMDNPSHIVPVMVGDAAKCKWISDILLDSHGVYVQPINYPTVPRKTERLRITPTPLHSDADIEHLVGALEA",
    "response#3": "MDFEAFFTTELQSLHSEGRYRVFADIERQQGNFPRATRYNANGQRKDVTVWCSNDYLGMGQNPKVIEAMKAAIDHCGAGAGGTRNISGTNHYHVLLEQELADLHGKESALIFTSGYVSNWATLGTLGQKIPGLIIFSDALNHASMIEGIRYGRCERVIWKHNDLEDLEAKLKAADPNAPKLIAFESVYSMDGDIAPIKEICDLADRYGAMTYLDEVHAVGMYGPRGGGIAEREGLMDRLTIIEGTLGKAFGVMGGYITGSTAVCDFIRSFASGFIFTTALPPSLAAGAIASIQHLKASPFERARHQDRVRKLRGLLDARGIPHMDNPSHIVPVMVGDAAKCKWISDILLDSHGVYVQPINYPTVPRKTERLRITPTPLHSDADIEHLVGALHQLWSHCALARL"
  },
  {
    "reference": "MSTNFFFAFESSEMILITLLFLGLFGRSNLVVSSSCILLCLKYFKLDQLVFPVLESRGLELGLVLLMLHILSPVATEKLTIKDLHSVTSLKGLFALAAGTLATKLNGDGLALMNARPEIIFGLTVGTVLGILFLRGTPCGPVMAAAVTAVFLQIASLFS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The designed protein localizes to the plasma membrane. The designed protein sequence is ",
    "response#1": "KGALLVCLAATMWGFDGIALTPRLFSLHVPFVVFILHLLPLILMSILFGKEEVKNIKKLQKNDLFFFFCVALFGGCLGTLCIVKALFLVNFKHLTVVTLLQKLQPIFAIILARLLLKEKLKRAYLFWGFLALLGGYLLF",
    "response#2": "KGALLVCLAATMWGFDGIALTPRLFSLHVPFVVFILHLLPLILMSILFGKEEVKNIKKLQKNDLFFFFCVALFGGCLGTLCIVKALFLVNFKHLTVVTLLQKLQPIFAIILARLLLKEKLKRAYLFWGFLALLGGYLLLA",
    "response#3": "ASLYSLLAAFSFGSATVFGKRILKSASFRTALYLRYLMTSCIMFVIVTFTSGFGDFLVATAGNWLIFVIIALTTGSGAILLYYFGLRYITAKVATMCELCFPISSVVFDYLINGNVLSPVQIASAILMIISIIKS"
  },
  {
    "reference": "MKYTKTLLAMVLSATFCQAYAEDKVWISIGADANQTVMKSGAESILPNSVASSGQVWVGQVDVAQLAELSHNMHEEHNRCGGYMVHPSAQSAMAASAMPTTLASFVMPPITQQATVTAWLPQVDASQITGTISSLESFTNRFYTTTSGAQASDWIASEWQALSASLPNASVKQVSHSGYNQKSVVMTITGSEAPDEWIVIGGHLDSTIGSHTNEQSVAPGADDDASGIAAVTEVIRVLSENNFQPKRSIAFMAYAAEEVGLRGSQDLANQYKSEGKNVVSALQLDMTNYKGSAQDVVFITDYTDSNFTQYLTQLMDEYLPSLTYGFDTCGYACSDHASWHNAGYPAAMPFESKFNDYNPRIHTTQDTLANSDPTGSHAKKFTQLGLAYAIEMGSATGDTPTPGNQLEDGVPVTDLSGSRGSNVWYTFELETQKNLQITTSGGYGDLDLYVKFGSKASKQNWDCRPYLSGNNEVCTFNNASPGTYSVMLTGYSNYSGASLKASTF",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. helix-helix-helix-helix-strand-strand-strand-strand-turn-helix-strand-helix-helix-strand-strand is the desired arrangement for the engineered protein.2. We need a protein that can perform Release of an N-terminal amino acid, preferentially leucine,   but not glutamic or aspartic acids. with high efficiency.3. Design the protein with a signal peptide incorporated.4. The protein must be able to metalloexopeptidase activity. The designed protein sequence is ",
    "response#1": "LTRDLVNTNADEVTPEKLAAVAKDLAGEFASLDVKILDRKAILKEKMGLLAAVAKGAAVEPRFIVLDYQGKPKSKDRTVLIGKGVTFDSGGLDLKPGKAMITMKEDMAGAATVLGIFSALASLELPINVTGIIPATENAIGSAAYKMGDVYVGMTGLSVEIGSTDAEGRLILADAISYALKYCNPTRIIDFATLTGAMVVSLGESVAGFFANNDVLARDLAEASSETGEALWRMPLVEKYDQALHSDIADMKNIGSNRAGSITAALFLQRFLEDNPVAWAHLDIAGTAYHEKEELPYPKYATGFGVRCLL",
    "response#2": "LTRDLVNTNADEVTPEKLAAVAKDLAGEFASLDVKILDRKAILKEKMGLLAAVAKGAAVEPRFIVLDYQGKPKSKDRTVLIGKGVTFDSGGLDLKPGKAMITMKEDMAGAATVLGIFSALASLELPINVTGIIPATENAIGSAAYKMGDVYVGMTGLSVEIGSTDAEGRLILADAISYALKYCNPTRIIDFATLTGAMVVSLGESVAGFFANNDVLARDLAEASSETGEALWRMPLVEKYDQALHSDIADMKNIGSNRAGSITAALFLQRFLEDNPVAWAHLDIAGTAYHEKEELPYPKYATGFGVRCLLRH",
    "response#3": "LTRDLVNTNADEVTPEKLAAVAKDLAGEFASLDVKILDRKAILKEKMGLLAAVAKGAAVEPRFIVLDYQGKPKSKDRTVLIGKGVTFDSGGLDLKPGKAMITMKEDMAGAATVLGIFSALASLELPINVTGIIPATENAIGSAAYKMGDVYVGMTGLSVEIGSTDAEGRLILADAISYALKYCNPTRIIDFATLTGAMVVSLGESVAGFFANNDVLARDLAEASSETGEALWRMPLVEKYDQALHSDIADMKNIGSNRAGSITAALFLQRFLEDNPVAWAHLDIAGTAYHEKEELPYPKYATGFGVRCLLE"
  },
  {
    "reference": "MDTVAIILAAGQSTRMRSRLPKVLHPIAGRPMIWYSLQAARQAVGEGRRPILVVGHAADAVRAAVGDQAEYAYQAQQLGTGHAVMQARPLLEGWQGTVLVLYGDMPLLRGETLRSLLQLHETQRSHTPLTMLTVESQDSMGFGRVVRDEAGRVLAVVEEAVATPEQKRITELNCGVYCFESAWLWEHLGRLPLSPKGEYFLTDLVAMAAEEGYAVATWKLADVSEVLGVNNRLHLAQVERVMRRRINERWMLAGVTMTDPETTYIDMDVEIGQDTVIFPNTHLLGTTRIGEECRIGPNSVIRDSTIGNRCTILASVLESALVEDDVDIGPFGHLRKGAHLAQGVHMGNFGEVKNAYLGPGTKMGHFSYIGDAQIGRNVNIGAGTITCNFDGKRKHQTIIEDDVFIGSDSLLVAPVRVGAGAITGAGSVVTHDVPPGAVVYGVPARVRRQREDIPPQNKEEEPPA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.3. The protein should be able to bind acetyl-CoA ligand in a variety of conditions4. A protein with UDP-N-acetylglucosamine diphosphorylase activity, magnesium ion binding is needed to enhance cell morphogenesis, cell wall organization, regulation of cell shape in a specific context. The designed protein sequence is ",
    "response#1": "RHVELGYRAVRVQSGVPGIETTYGVAKTPGERYEPADSSLPAEHVWSTEKYLNHAPKLFAAVRERFGDDLHVLHDVHHRLTPIEAARLGKAVEPYHLFWLEDCVPAENQESLRLIREHTTTPLAIGEVFNSIHDCRELIQNQWIDYIRMPLTHGGGITAMRRVADLASLYHVRTGFHGPTDLSPVCLGAAIHFDTWVPNFGIQEHMPHTDETDAVFPHDYRFEDGHFLAGESPGHGVDIDER",
    "response#2": "IEDCLGEVARHVELGYRAVRVQSGVPGIETTYGVAKTPGERYEPADSSLPAEHVWSTEKYLNHAPKLFAAVRERFGDDLHVLHDVHHRLTPIEAARLGKAVEPYHLFWLEDCVPAENQESLRLIREHTTTH",
    "response#3": "RHVELGYRAVRVQSGVPGIETTYGVAKTPGERYEPADSSLPAEHVWSTEKYLNHAPKLFAAVRERFGDDLHVLHDVHHRLTPIEAARLGKAVEPYHLFWLEDCVPAENQESLRLIREHTTTPLAIGEVFNSIHDCRELIQNQWIDYIRMPLTHGGGITAMRRVADLASLYHVRTGFHGPTDLSPVCLGAAIHFDTWVPNFGIQEHMPHTDETDAVFPHDYRFEDGHFLAGESPGHGVDIDEG"
  },
  {
    "reference": "MTLSISDQNKLDSFWSYCVKNQYFNIGYPESADFDYTILERFMRFSINNCGDWGEYCNYLLNSFDFEKEVMEYFAQLFKIPFEESWGYVTNGGTEGNMFGCYLGREIFPNGTLYYSKDTHYSVAKIVKLLRIKSTLVESQPNGEMDYADLIKKIKRDNEKHPIIFANIGTTVRGAIDNIAIIQQSISELGIERKDYYLHADAALSGMILPFVDNPQPFNFADGIDSIGVSGHKMIGSPIPCGIVVAKKKNVDRISVEIDYISAHDKTISGSRNGHTPLMMWEAIRSHSWEEWRRRIERSLNMAQYAVDRFQSAGIDAWRNKNSITVVFPCPSEAVWKKHCLATSGDIAHLIATAHHLDSSKIDALIDDVIADLKKQAA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The substrate binding site should be located in a region of the protein that is accessible to the ligand.2. The protein design should be able to enhance the efficiency of carboxylic acid metabolic process.3. The desired protein should be able to perform H(+) + L-histidine = CO2 + histamine4. The protein must be engineered to have a cooperative binding mechanism with pyridoxal 5'-phosphate to ensure proper regulation. The designed protein sequence is ",
    "response#1": "GSQGIVQGTYETFVEAGRRHYGGSLAGRWILTGGLGGMGGAQPLAATMAGASMLAVECQPSRIEARLRTGYLDRQTADLDQALAWIAEAGAPGAKPVSVGLLGNAAEVFPELVKRGVRPDLVTDQTSAHDPLNGYLPAGWSLERWERGRERAPAEVIAAAKASMATQVRAMLAFHAQGIPTVDYGNNIRQRALEEGVSDAFAFPGFVPAYVEPYRGTDPLFCEGKGPFRWAALSGDPEDIYRTDALLKELFPENQALHRWIDMAQKKVTFQGLPSRICWLGYGEREKMGLAINELVRNGELKAPIVIGRDHLDCGSVASPNRETEAMKDGSDAVGDWAVLNALINTASGASWVSFHHGGGVGMGYSLHAGMVAVADGSILAEQRLSRVLTSDPGMGIIRHADAGYEKAAQVADKLG",
    "response#2": "GSQGIVQGTYETFVEAGRQHYGGDLAGRWILTGGLGGMGGAQPLAATMAGASMIAVECTPSRIEMRLRTRYLDRRADTLDEALAMLEAAKRDGKPVSIGLLGNAAEVFPELVRRGIRPDIVTDQTSAHDPVNGYLPAGWTLEHWAAMRERDPEAVALAAKRSMAGQVRAMLDFWRMGIPVLDYGNNIRAMAQEMGVADAFDFPGFVPAYIAAKK",
    "response#3": "PLFCRGIGPFRWAALSGDPEDIYRTDAKVKELIPDDPHLHTWLDMARERIHFQGLPSRICWVGLGDRHRLGLAFNAMVASGELKAPVVIGRDHLDSGSVASPNRETEAMRDGSDAVSDWPLLNALLNTAGGATWVSLHHGGGVGMGFSQHAGMVIVCDGSEDAARRIGRVLWNDPATGVMRHADAGYDDAIACAREVCV"
  },
  {
    "reference": "MLLKELIKEIENLDIRGSTNIKVNNVSKNSNEIKPGDLFVAIKGSKSDGHDFINDAIIKGAKCILSEKIIDVEDDNLTFVITKCSKETYSRVCSXFFDNPSNKLKLIGITGTNGKTTSSNLLYQMFTINNIKSGLISTNEIIIGSDKFETSLTTPDSYDLNFYLNKMVSNGIRFCFMEVSSHAIDQRRISGLNFDLAIFTNITHDHLNYHKTFKNYLNVKKRFFDDLVKDSISLINVDDKNANYIVQNTISKVYRLSLLKDADYKLKIIENSFTGLMIKVDNELINTQLIGKFNAYNLLTVLSASKLLNLESKKILVGLSMLKNPDGRFDVVQMNKIIGIVDYAHTPDALLNVLETISDIKNNENSIITVVGCGGGRDKEKRFKMGRIASKYSNKVIFTSDNPRDEDPKLIIDDMILGVDDEDKKKVINIVDRKKAIERASKIVKPNDILLIAGKGHENYQIIKSKKIEFNDKNILKETLKMAV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a binding site that is specific to the target ATP, meso-2,6-diaminoheptanedioate ligand.2. The designed protein should have magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, ATP binding within cytoplasm to regulate cell cycle, peptidoglycan biosynthetic process, cell wall organization, regulation of cell shape.3. The protein design should take into account the physiological conditions of the Cell wall biogenesis, peptidoglycan biosynthesis pathway.4. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.5. The protein's functional design necessitates the inclusion of a Meso-diaminopimelate recognition motif motif.6. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.7. The protein contains novel Mur ligase N-terminal catalytic, Mur ligase central, Mur ligase C-terminal domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MKLKDLLNGIEYSFIRGNVDVEIANIRFDSRLVEQNDLFFALKGTRIDGRRFIGQAMERGACAVVCEHPSVPDGVSQVIVPDTRLALSLIASKFYNNPSRKLKLVGSIGTNGKTTTSYMVEAILKNAGYKTGLIGTIKNVIDGKEYENAHTTPDILEIHKILSRMVDAGVSHCVMEVSSHALALDRVYGCDFDIGVFTNLTQDHLDFHKTFENYFQAKCKLFTNCLNKDGKKKSAVINIDDEYGKRLKEETSAKILTFGINNKADISAENIKISAKGTSFILITHAGVYEIKTSLVGKFNIYNILAAVSAAILEGISIEAIKKALEDIKGVDGRFETIGLNGGVTAIIDYAHTPDALENVLTTIDRIRGKGQRIITVVGAGGDRDKTKRPLMGKIAARSSDLVILTSDNPRSEDPEGIIEDMRGGIEDKDQQKVTRTVDRKRAIETALSLADRGDIVLVAGKGHETYQILKDHTIPFDEREVVREIIKNGGK",
    "response#2": "MKLRELIRELTLDSRQVRPGDLFLAVPGAQLDGRQHIADAMSRGAAAVAYEEQGATVLPLTDIPLIPVRGLIAQLSEIAGRFYGDPSRELWLVGVTGTNGKTTTTFLIEQGLRGVGERAARLGTLGFEMEGHVFGDTLTTPEADDIARCLLEVQQRGGRYLVMEVSSIAVVQERVSGLRFRAAAFTNLTQDHLDFHGSLEAYAEAKAALFTRLAPELSVLHVGDAFGRELSRRVHSGRLTVARQEADVRVAEASLGASGIRAKLITPQGDCSIDSALVGEHNLENLLVALGALLALGVPLPALVAGLQAVPGRLERVGEHNGAPIFVDYAHKPDALAKALLALRPYAKRRLVVVFGAGGDRDAGKRPLMGAIASKNADDIIVTDDNPRSESPDAIRAAILSAAKGAREIGDRAAAIRVAIEALQPGDALLIAGKGHEDYQILGDRRLHFSDRDVARALLQG",
    "response#3": "MVLVNGLQLDSRQIKTGEMFIAYPGHATDGREYIQQAVDTGASVVLFEQDRSETILKTLLNDNQQVAASAKTLSGVYNYKEAVLIPVKNLSQNVSLIAARFYQNPSSKLKMIGVTGTNGKTTTTFLIEHLAAKAGLSTALFGTLANRWPGCNITATHTTPFADQLQAQLAAALATGVQLAAMEVSSHALHQQRVAGCNFSGAVFTNLSQDHLDYHPSLEAYYEAKAMLFEPIYLKGMAVINSDDPHGKRLAAHLAAKCWRSSLLDPTAELFMDALQFSAAGVAGVIHSPKGAAAFQSPLVGRFNLMNLLQAVGAGLALGIPLAIIKKGLESVKGIDGRMERVPHGPGQPAVLVDYAHTPDGLANALGACKPFAQGRLICLFGCGGDRDRGKRPLMGEIAARLADQVVLTSDNPRTEDPQQILNDVLAGMPAGSNMIVEPDRGLAIQKAIALGGSGDLVLIAGKGHENYQIIGSKKLYFDDREEARKALEL"
  },
  {
    "reference": "MKPNLALVGATGAVGTVMIDIINNRENIWGEIRLIASARSAGKKIAVYGKELTVVALSPEAFDGIDIAMFDVPDEISAEWGPIAAAKGVVVVDNSAAFRMDPEVPLVVPEVNPEMAKSRPKGIIANPNCTTLTMMAALGALHKKWQLTELVVASYQAVSGAGSSGIEQLKKETAAVATNAEAGLASDTVAKTLKQQGLNSSDSLWPTPIALNVIPLAGSVKEGGHTSEELKVRNESRKILGIKDLKVAATCVRVPVQVSHSLAVHATFANDITVEQAKAELEKQPTVVLMDEPEKGIFPTPAFVAGQDPTYVGRMRQSMDFPKTLDMFVVGDNLRKGAALNTYEIAELVAKELA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must possess NADP binding to support diaminopimelate biosynthetic process under certain conditions.2. The designed protein should have a binding site that is specific to the target phosphate ligand.3. The protein design should consider the endogenous proteins and enzymes present in the L-threonine biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "GLFHAGLVEWVSAMTYQAASGAGAKNMRELLEQMGALHASVRPLLADPASAILDIDRGVTETMRDAGFPTASFGAPLAGSLIPWIDTQLENGQSREEWKGQAETNRILGLETAPIPVDGLCVRVGAMRCHSQGLTIKLTRDVPLPEIEQLIAGANEWVRVIPNDRSLTVRELSPAAVTGTLSVPVGRLRKMNLGPDYLSAFTVGDQLLY",
    "response#2": "GLFHAGLVEWVSAMTYQAASGAGAKNMRELLEQMGALHASVRPLLADPASAILDIDRGVTETMRDAGFPTASFGAPLAGSLIPWIDTQLENGQSREEWKGQAETNRILGLETAPIPVDGLCVRVGAMRCHSQGLTIKLTRDVPLPEIEQLIAGANEWVRVIPNDRSLTVRELSPAAVTGTLSVPVGRLRKMNLGPDYLSAFTVGDQLLY",
    "response#3": "GLFKADLIEWMTSMTYQAASGAGAENMRELVAQMGAAHGAARKLLDDPASAILDIDRAVAAEIRSKDHPTRAFGAPLAGSLLPWIDKDLGNGQSREEWKGGAEGNKILGLDRRPIPIDGICVRVGAMRCHSQALTIKLRRDVPLQDIEGLIAGANEWVRLIPNQREATLAELTPAKVTGTLSVPIGRLRKVELEGGGAHLIAFTVGDQLLIR"
  },
  {
    "reference": "MSSYFLNSFSGRYPNGSDYQLLNYGTNGAMNASYRDSTSMHSGSFGYNYNGIDLSVNRPNNGHFGAVGDNSRAFQNPSQETRYRQSSCSLSSPDPLSCATSDTLELKGSSPPSDQSTPTGNSRLTNSNNLTSSNSTHFADTDETNVSSETEEGAQTTTTSHTQKQESVATSTTTPNDGQTPQIFPWMRKLHISHDMTGPDGKRARTAYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLSERQIKIWFQNRRMKWKKDNKLKSMSLATAGSAFQN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein's functional design necessitates the inclusion of a Antp-type hexapeptide motif.2. A protein that contributes to brain development, anterior/posterior pattern specification, heart development, cardioblast differentiation by functioning in nucleus with DNA-binding transcription factor activity, RNA polymerase II-specific, RNA polymerase II cis-regulatory region sequence-specific DNA binding.3. The protein must exhibit the following characteristics: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis..4. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "MYPSPVRHPSLNLNPQNYVPGPPQYSDFASYHHVPGINNDPHHGQPAAAWGSPYTPAKEDWHSYGTAAASAATNPGQFGFSPPDFNPMQPHAGSGLLPPAISSSVPQLSPNAQRRTPYEWMRRSIPSTSX",
    "response#2": "MYPSPVRHPSLNLNPQNYVPGPPQYSDFASYHHVPGINNDPHHGQPAAAWGSPYTPAKEDWHSYGTAAASAATNPGQFGFSPPDFNPMQPHAGSGLLPPAISSSVPQLSPNAQRRTPYEWMRRSIPSTSS",
    "response#3": "MYPSPVRHPSLNLNPQNYVPGPPQYSDFASYHHVPGINNDPHHGQPAAAWGSPYTPAKEDWHSYGTAAASAATNPGQFGFSPPDFNPMQPHAGSGLLPPAISSSVPQLSPNAQRRTPYEWMRRSIPSTSH"
  },
  {
    "reference": "MFTYIKEIEEAWQIKDKLLQDSVKLSAFKKTLQDVIENLNKGTIRVCEKQNSSWQVNDWVKKAILLYFMTTESQLYNNNYNSWYDKVAPKFPFDAELNKFKEAGIRKVPGSFVRTGTYIAKNVVIMPSFINIGAYIDEGTMIDTWATIGSCAQIGKNCHISGGTGIGGVLEPLQAKPVIIEDNCFIGARSEIAEGVIVEEGAVVSMGVFIGASTKIVYRDSGEIIYGIVPACSVVVPGVLPNKEADKPGLYCAVIVKQVDKNTRAKVSINELLRS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase activity within cytoplasm to regulate diaminopimelate biosynthetic process, lysine biosynthetic process via diaminopimelate.2. The protein should be optimized for activity in L-lysine biosynthesis via DAP pathway, Amino-acid biosynthesis, LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 1/3 pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MKTLVSAIEDAFARYSTLTVEEIEDSIRPTVNRVIDGLETGAFRVAEPDNHGGWKVNEWLKKAVLLYFRK",
    "response#2": "MSSLQQTIEQAFENRNEYSPATMPQDVRDAINQVLEQLDNGTLRVAEKKDGEWVVNQWAKKAVLLSFRH",
    "response#3": "MSSLQQTIEQAFENRNEYSPATMPQDVRDAINQVLEQLDNGTLRVAEKKDGEWVVNQWAKKAVLLSFRH"
  },
  {
    "reference": "MGREVVEVLMDRNADVSSARVHVAPKIAAEETDEEFEVKECTEEKSLSENAPNVGSAERVGAQKSPKTRNGNAKVSKQDAPLLAVRKPLQPENKKHIDDEDNCSIASSVATSMRMGKSGLTYGSAPTFRSAQRAEKRKEYYQKLEEKNQALEAERNELEQRQKDEQEAALKQLRKNLKFKAKPVPNFYYEAPPAKPELKKLPLTRPKSPKLILSRRKSFSDAVSSSSREEILKTVSNRNRHSTGTVQNKDDDHRNKNTNAAHDSPRVRSGKGKSGLKPVNESSEEA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein localizes to the microtubule.2. For general function, the protein need meet that Microtubule-associated protein (MAP) that regulates the orientation of interphase cortical microtubules The designed protein sequence is ",
    "response#1": "LNGHLKALKDYFLLAKGDFFQCFLEESRQLMRLPPRQSTAEADLMVPFQLASLKTIGEEDKYFSKVSLRMPSYGITVKPSLLNVPKATSAAADGISGASISNASSEMSVDGWDGIALEYSIEWPLHLFFTQEVLSRYLKVFQYLLRLKRTQMELEKLWASVMHQYHSIFAKNKKSDQDKSPITQQRDQRFRSMWRVREHMAFLIRNLQFYIQVDVIESQWNILQSHIQDSHDFTELVGFHQEYLSALISQTFLDIGSVSRILDGIMKLCLQFCWNIENQDNFSNTSELEHIAEEFNKKSNSLYTILRSSRLAGSQRTPFLRRFLLRLNLNSFF",
    "response#2": "LNGHLKALKDYFLLAKGDFFQCFLEESRQLMRLPPRQSTAEADLMVPFQLASLKTIGEEDKYFSKVSLRMPSYGITVKPSLLNVPKATSAAADGISGASISNASSEMSVDGWDGIALEYSIEWPLHLFFTQEVLSRYLKVFQYLLRLKRTQMELEKLWASVMHQYHSIFAKNKKSDQDKSPITQQRDQRFRSMWRVREHMAFLIRNLQFYIQVDVIESQWNILQSHIQDSHDFTELVGFHQEYLSALISQTFLDIGSVSRILDGIMKLCLQFCWNIENQDNFSNTSELEHIAEEFNKKSNSLYTILRSSRLAGSQRTPFLRRFLLRLNLNSFV",
    "response#3": "LNGHLKALKDYFLLAKGDFFQCFLEESRQLMRLPPRQSTAEADLMVPFQLASLKTIGEEDKYFSKVSLRMPSYGITVKPSLLNVPKATSAAADGISGASISNASSEMSVDGWDGIALEYSIEWPLHLFFTQEVLSRYLKVFQYLLRLKRTQMELEKLWASVMHQYHSIFAKNKKSDQDKSPITQQRDQRFRSMWRVREHMAFLIRNLQFYIQVDVIESQWNILQSHIQDSHDFTELVGFHQEYLSALISQTFLDIGSVSRILDGIMKLCLQFCWNIENQDNFSNTSELEHIAEEFNKKSNSLYTILRSSRLAGSQRTPFLRRFLLRLNLNSFL"
  },
  {
    "reference": "MAKRVAIIGGGSSGLCAIKACLQEALEPVCFERSGDIGGLWRFEECPEEGRASIYRSVIINTSKEMMCFSNFPIPEDFPNYMHNSKIMEYFRMYAQHFDLLRHIRFRTSVCRVSKRPDFAATGQWEVVTESEGKQEAAVFDAVLVCTGHHTEAHLPLNTFPGINKFKGRYLHSRDYKDTQDFTDKRVVVIGIGNSGADLAVEVSQTAKQVSSRSGVPLARAAPHQGYPIDTVAITRINAFRKKLLSHSMVSNFVENTLMVPCTFRVFDQHPTINDDLPNRIISGRVLVKPNIQEFTETSAIFEDGTREDIDVVIFATGYSFSFPFLEGCVNVVENQIPLYKFMFPPDLEKPTLAFIGLIQPLGAIMPISELQCRWATRIFRGLNKLPPQCYMEADIKQKREAMAKQYVKSQRHTIQVDYIPYMDELACQVGVKPNLLTLFLSDPKLALEVAFGPCTPYQYRLRGPGKWAGARDAILTQRQRIIKPLQTRHVEEHLSAPSMPLLFKLVGAVAVLAIGFAYL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Acts as Baeyer-Villiger monooxygenase on a broad range of substrates The designed protein sequence is ",
    "response#1": "EFPICAFSHCRDVVAAVSNAGGFGILGAVAHSPKRLESELTWIEEHTGGKPYGVDVLLPPKYIGAEQGGIDAQQARELIPEGHRTFVDDLLVRYGIPAVTDRQRSSSAGGLHISPKGYQPLLDVAFAHDIRLIASALGPPPPDLVERAHNHDVLVAALAGTAQHARRHAAAGVDLIVAQGTEAGGHTGEVATMVLVPEVVDAVSPTPVLAAGGIARGRQIAAALALGAEGVWCGSVWLTTEEAETPPVVKDKFLAATSSDTVRSRSLTGKPARMLRTARL",
    "response#2": "DFFWFIPTHGDGSYLGSEEQQRPPEFGYFKQIAQAVDRLGFPGVLLPTGQNCEDSWITATGLATLTEKLKFLVALRPGVTLPTFAARQTAALDRLSNGRLLLNVVVGGNPTELAGDGVFLPHDERYAQAHEFLTIWRGLVSGERVNFDGKYYRVENGRLDLLPSQERPPLYFGGSSDAGQDLAADLVDMYLTWGEPPALVAEKLASARKKAALRGRKLRFGIRLHFIVRETEDEAWRAADRLISHVTDAQIENAQARFLNQMDSVGQRRMAELHGGRRDRLVVSPNLWAGVGLVRGGAGTALVGTPEQVTERIREYQAIGIERLRR",
    "response#3": "IDVIIAGGGPTGLMLAGELRLHGVRTVVLEKEPTPNQHSRSRGLHARSIEVMDQRGLLERFLAHGEQFRVGGFFAGLAAEWPADLDTAHSYVLAIPQVVTERLLTEHATELGAEIRRGCEVAGLDQDADGVTAELADGTRLRARYLVGCDGGRSTVRRLLGVDFPGEPTRVETLLADVRIDVPVETLTAVVAEVRKTQLRFGAVPAGDGFFRLIVPAQGLSADRAAPTLDELKRCLHATAGTDFGVHSPRWLSRFGDATRLAERYRTGRVLLAGDAAHIHPPTGGQGLNLGIQDAFNLGWKLAAAIGGWAPPDLLDSYHDERHPVAAEVLDNY"
  },
  {
    "reference": "MTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLNVTIQKFDPYLNVDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFIDINLNKYSNITTGKIYSSVIRKERRGDYLGGTVQVIPHITNEIKNKVFQAGKETNADVVITEIGGTVGDIESLPFLEAIRQLKSDLGRENVMYIHCTLVPYIKAAGELKTKPTQHSVKELRSLGIQPDVIVLRTEMAISKDMKEKIALFCDINENAVIEARDCDVLYEVALEMQNQGLDQLACDHFHINCQDANMEEWDELINQVKNLSKTTTIALVGKYVALQDAYISVVEALKHAGFAHDADVNVKWVSSEELNEHNAEDILGDVDGILVPGGFGDRAIEGKIAAITFARENKIPFLGICLGMQLATVEFARNVLGLEGAHSAEINPTTPHPIIDLLPEQKDVEDLGGTLRLGVYACRLQEGTKAHEAYGDEVVYERHRHRYEFNNEYRQQMEEQGFVFSGKSPDDRLIEIIELTDHPWFVASQFHPEFKSRPTRPQPLFRDFIGHSIK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should take into account the physiological conditions of the Pyrimidine metabolism, CTP biosynthesis via de novo pathway, CTP from UDP: step 2/2 pathway.2. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.3. The protein with the reaction H2O + L-glutamine = L-glutamate + NH4(+), the Nucleophile; for glutamine hydrolysis, active site must be optimized to ensure efficient catalysis.4. The presence of CTP synthase N-terminal domains is critical for the interaction of the designed protein with its intended targets.5. The designed protein must possess ATP binding, glutaminase activity, metal ion binding to support glutamine metabolic process, 'de novo' CTP biosynthetic process under certain conditions.6. The protein should have a high affinity for Mg(2+), ATP, L-glutamine, CTP. The designed protein sequence is ",
    "response#1": "MTKYVVVSGGVLSGIGKGVTASSIGVLMKSLGLRVTAIKIDPYLNSDAGTMSPFEHGEVFVLDDGGEADLDLGNYERFLDVTLSRDNNLTTGKIYSEVIGKERKGDYLGKTVQVVPHITDEIQDWIERVAHTPADGTDEIPEICVIELGGTVGDIESAPFVEALRQFQFRVGKNNICFLHVSLVPVIGVVGEQKTKPTQHSVKGLMASGIQPDFLVCRSSEPLLPEVKRKLSLFCHVSEQAVFSAHDVSNIYRVPLMLEEQGAIVQMAAHLPDAYMSVVEALRHGGLCYDSAIDIKWVNAVELEKGEDIVNEQLACADGILVPGGFGDRGIEGKIRAITYARENKIPFLGICLGMQCAVVEFGRYIGLKKANSSEFDPKTPHPVIDLLPEQKDIEDKGGTMRLGLYPCKMAEGTKAYESYKEGLIYERHRHRFEFNNEYRDLMISKGMIISGTSPNERLVEIIELKDHPWFIGTQFHPEFKSRPNRPHPLFRDFMSKALAYGK",
    "response#2": "MTKYIFITGGVVSSLGKGIASASIGLILKSRGLKVTILKFDPYINVDPGTLSPFQHGEVYVTNDGAETDLDLGHYERFIDEDMTRKNNATTGQIYYTVITKERRGDYLGKTVQVIPHITDEIKKRIREAGRPDEVYDVVIAEVGGTVGDIESLPFLEAIRQFCLDVGQENSLNVHLTLVPFIKAAGEFKTKPTQHSVMRLREIGIQPDIMLCRCEMHLDEEAKNKIALFCNVPKEAVIEARDVPTIYEVPLIFEEGHVGDLIIESLGLECPVADLSEWEQVVDQVTNPQGEVTIGVVGKYIDLPDAYLSVVEAIRAGGFAHKTKVSIRWIASDDCATEEGAQKQLADLDGICVPGGFGIRGIEGKVGALRFAREHGIPTLGLCLGLQCMVIEYARNVAGLEGASSSEFDPDSPHPVIATMTEQVDIVSGNGDLGGTMRLGLYEASLQKGSVVRDLYGEDSIQERHRHRYEVNNSYRDALTAAGLVISGQSPEGNLVEFVELPAREHPFYVATQAHPELRSRPNRAHPLFVGLVQAALAQQA",
    "response#3": "KFIFVTGGVVSSLGKGIAAASIGALLENRGLKITLQKFDPYINVDPGTMNPFQHGEVFVTDDGSETDLDLGHYERFTGAKMTRRNNFTTGKIYDTVIQKERHGDYLGHTVQVIPHITDEIKASIMKVAGGVDLVIIEIGGTVGDIESLPFLEAIRQFKTDVGEENVLYAHVTLVPHIDAAGELKTKPTQHSVQKLREIGIQPDILLCRTDRDLSKEIRAKISLFCNLPANCVFSAKDAKSIYEVPLNFHEEGVDEKIVELLNIWTKAPDLSVWENIVKRIKNPPHKLPDAYLSVVEALKHAGYEHDTNIEIEWIQSENVTKENVSDYLSTVDGVLVPGGFGDRGIEGKIEAIRFARENNIPFLGICLGMQMACVEFARNVLMLEGADSKETAPETPYNIIDLMLDQEGVEDMGGTLRLGAYPCELKPDTKTRDLYNGEELISERHRHRYEFNNAFRESFEQAGMVFSGVSPDNRLVEIVEIPANDFFVASQFHPEFKSRPNRSHPLFNGFILAAIDYQNK"
  },
  {
    "reference": "MNGETPDASHPQQSRAELTLAALGVVYGDIGTSPLYAVKETFNPAHGIPLVTENILGGISAILWALMVVVSLKYVILIMRANNRGEGGIMALLALALSSVKKVGRSPTPILLVGLFGAALFYGDAVLTPAMSVLSALEGIEVGTTALQPYVLPASVGVLIALFLFQRHGTAAIGALFGPVTIVWFLALAAAGIHGIARYPAILGALSPLHALGFVTQHGFASFAVLGAVLLAFTGAEALYADMGHFGSAPIRLAWFGLVFPALALNYLGQGALIIVNAKAIENPFYLLYPSWALYPMVALATAATVIASQATISGAYSLTKQGIQLGYLPRMNVVHTSERAIGQIYIPTLNGMLLVAVLVAVLGFGSSSNLASAYGVAVTGTMLVTTLLTFFVIHYGWRYNLLLSLVATGFFIAVDMAFVSSSLLKVAEGGWFPLVVGAGIFVVMLTWVRGRQALLERLQSTDVPLKSFLDSLFLAPPPRVPGTAVFLTPTPDVVPHALMHNLNHNRVLHERVVFLTVKMKDVPSVPATECAAVEPLGHACYRITLRFGFMNRPDVAQALGALPPAAGLEFDIMDTSFFLSREAIVAAAGGPSGMASWRERLFATMSRNAGNAADYFNIPANRVIEIGTQIKI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be expressed specifically in the plasma membrane The designed protein sequence is ",
    "response#1": "AFHDHILMPIITAITLLVLGLLIWIVIRYNAKANPVPAKFSHNTTVEIIWTIGPVLILMVIAIFSFRLLFAYNDMPKPYLTVKATGYQWYWGYEYPDQKISEITSVPLKQEEAVAKGVPYLLAADKPMVVPVNQVIRVQVTGADVIHAFALPAFGLKTDAIPGRLNETWFKADKIGVYYGQCSELCGVDHSNMPIEIHVVSQADFDAWV",
    "response#2": "AFHDHILMPIITAITLLVLGLLIWIVIRYNAKANPVPAKFSHNTTVEIIWTIGPVLILMVIAIFSFRLLFAYNDMPKPYLTVKATGYQWYWGYEYPDQKISEITSVPLKQEEAVAKGVPYLLAADKPMVVPVNQVIRVQVTGADVIHAFALPAFGLKTDAIPGRLNETWFKADKIGVYYGQCSELCGVDHSNMPIEIHVVSQADFDAWR",
    "response#3": "AFHDHILMPIITAITLLVLGLLIWIVIRYNAKANPVPAKFSHNTTVEIIWTIGPVLILMVIAIFSFRLLFAYNDMPKPYLTVKATGYQWYWGYEYPDQKISEITSVPLKQEEAVAKGVPYLLAADKPMVVPVNQVIRVQVTGADVIHAFALPAFGLKTDAIPGRLNETWFKADKIGVYYGQCSELCGVDHSNMPIEIHVVSQADFDAWV"
  },
  {
    "reference": "MFSPDGGLPAAPFGLLPDGGPPFPRGGYDGAAAQQLFFPFASEPDGARDAATARAWLPPPAGPPAKAEARPARPCRQGSPEPRAAPPATPCCGPAWAAPPWPGPAPPAATAVPGPPFPGPAAAAFPAAPGHALCPAALQPSSGGLANLGSSGSSSGAASEGGHSSDSGDEDAPTSEELEQFAKDLKHKRIMLGFTQADVGLALGTLYGKMFSQTTICRFEALQLSFKNMCKLKPLLQRWLNEAENTDNMQEMCNAEQVLAQARKRKRRTSIETNVKGTLESFFRKCVKPSPQEISQIAEDLNLDKDVVRVWFCNRRQKGKRLLLPFGNESEGVMYDMNQSLVPPGLPIPVTSQGYSLAPSPPVYMPPFHKAEMFPPPLQPGISMNNSSH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should contain at least one Homeobox domain that can specifically recognize and bind to DNA.2. A protein with RNA polymerase II cis-regulatory region sequence-specific DNA binding functions is required to enable regulation of transcription by RNA polymerase II.3. The designed protein must have at least one POU-specific domain. The designed protein sequence is ",
    "response#1": "MYPGTLRSPGGSSTAGVGTSGGSGSPLPASNFTAAPVYPHYVGYPHMSNMDPHGPSLGAWSSPYSPPREDWSTYPGPPSTMGTVPMNDMTSPVFGSPDYSTLGPTSGASNGGSLPDAASESLVSLDSGTSGATSPSRSRHSPYAWMRKTVQVTGKH",
    "response#2": "MYPGTLRSPGGSSTAGVGTSGGSGSPLPASNFTAAPVYPHYVGYPHMSNMDPHGPSLGAWSSPYSPPREDWSTYPGPPSTMGTVPMNDMTSPVFGSPDYSTLGPTSGASNGGSLPDAASESLVSLDSGTSGATSPSRSRHSPYAWMRKTVQVTGKK",
    "response#3": "MYPGTLRSPGGSSTAGVGTSGGSGSPLPASNFTAAPVYPHYVGYPHMSNMDPHGPSLGAWSSPYSPPREDWSTYPGPPSTMGTVPMNDMTSPVFGSPDYSTLGPTSGASNGGSLPDAASESLVSLDSGTSGATSPSRSRHSPYAWMRKTVQVTGKR"
  },
  {
    "reference": "MTFLEEILAQKAVEVAEMPLEQVAEKRKTYSFYDFLKENTEQMQLIAEVKRASPSKGEINMDVNPVAQAKSYEAAGAGMISVLTDPIFFKGSIEDLREVAKNVAIPVLCKDFIISEKQLIRARNAGATVVLLIISALTEDELALLFKQASALDLEVLVEVHDRDELALAQKIGAKLIGVNNRNLHTFEVDIAVSEMVANDFSTEACFISESGFKTADDVNRVSKEFNAVLVGEALMRDKTPQKAAESLKVKR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Ideal protein should have a binding affinity for key enzymes in the L- tryptophan from chorismate: step 4/5, L-tryptophan biosynthesis, Amino-acid biosynthesis pathway to enhance the overall efficiency of the pathway.2. The designed protein must be optimized for indole-3-glycerol-phosphate synthase activity.3. We need a protein that can perform 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+)   = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O with high efficiency. The designed protein sequence is ",
    "response#1": "MVIFGLSRAIRKAKKNPIIAEIKVYSPKYGDLLRGRDPLRILRAYEEAGAVGISYITDQKYFKGSFDFLKVLCKETTLPVLRKDFITSKEEIEKTAEVGASAVLLITRILKDKLPEFVDYAKEHGLDTLVEVHTEDELKLAIKTNSTMIGINNRDIGKLEMDDGDVSLTEILAPKIPGKFVKVSESGIATLEDLKRALRVADAALIGTALMKAENPAELLK",
    "response#2": "MILDEILKKTREDLKRRKKMLSFELLGRSLSANPYMPRDVIKALKSTPNEPFKLICEIKKASPSKGVIREYFKPVEIAREYEKAGADAFSVLTEPHFFKGDLEFISQIRRYTKMPILRKDFIIDEYQILEALVYGADFVLLIAKALSLKELKKLLEFTHHIGLEALVEVHDKKDLLNATLSGANIIGINHRDLNDFSLHMNLAEELVPLIPNGKIIVAESGLNSREQLVNLNKVGVDAFLIGEHFMRQNDICAAVREMKAGA",
    "response#3": "LDEILKKTREDLKRRKKMLSFELLGRSLSANPYMPRDVIKALKSTPNEPFKLICEIKKASPSKGVIREYFKPVEIAREYEKAGADAFSVLTEPHFFKGDLEFISQIRRYTKMPILRKDFIIDEYQILEALVYGADFVLLIAKALSLKELKKLLEFTHHIGLEALVEVHDKKDLLNATLSGANIIGINHRDLNDFSLHMNLAEELVPLIPNGKIIVAESGLNSREQLVNLNKVGVDAFLIGEHFMRQNDICAAVREL"
  },
  {
    "reference": "MGGQLDVLDIVVLGVLALGTIAYFTKGKYWGIVKDPYATSYATANGNKPAKTRNIVEKMEESNKNCVVFYGSQTGTAEDYASRLAKEGKSRFGLETMVADLEDYDFDNLDSMPDDKVAMFVLATYGEGEPTDNAVDFYEFIMGEDIEFSSGEKSLANLKFVAFGLGNNTYEHYNSMVRNVTKALEKLGATRIGAAGEGDDGAGTMEEDFLAWKDPMWKSLAESLGLQEREAVYEPVFAITDRDDLTKESKEVYLGEPNKLHLEGTSKGPYNAHNPYIAPIAESKELFTVNNRNCLHMEIDISGSNLSYQTGDHIAVWPTNAGKEVNRFLNVTGLLPKRDSVISVKPLESTAKVPFPTPTTYDAIARYHMEICAPVSRQFIATLAPFAPNETCKAEMEKLGGDKDYFQEQISTHYLNIAQVLEKVGGQEKWSSIPFSAFIEGLGKIQPRYYSISSSSLVQKKKISITAVVESMDIPGRTDALKGVTTNYLLALKQKQHGDDHPDPHGLTYEITGPRNKYDGVHVPVHVRHSNFKLPSDPSKPVIMVGPGTGVAPFRAFVQERAHQAKNGENVGRTILFFGCRNSNEDFLYKDEWKQYEEALGDKFSLITAFSREGPEKVYVQHRLKEQAKEVNDLLMQKAYFYVCGDAANMAREVNTVLAKIISEQRDIPESKAEEIVKGMRSSNQYQTAAHTETNNFPNRSTAATMYSQQQARANRDSQGQQGSYAPTSAGVSQTESGPMSAMLNQFAGLSIPGTMANGANPMAANQL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Target a Polar residues compositional bias in the protein's composition for improved properties.2. The designed protein should have a high specificity for FMN, FAD binding, with minimal off-target effects.3. The protein contains novel RRM, FAD-binding FR-type, Flavodoxin-like domains that confer a unique function or activity.4. The protein design should incorporate a binding pocket that specifically recognizes FMN to enhance enzymatic efficiency.5. For general function, the protein need meet that This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes; Involved in ergosterol biosynthesis.6. A protein that contributes to ergosterol biosynthetic process by functioning in plasma membrane, mitochondrial outer membrane with FMN binding, RNA binding, flavin adenine dinucleotide binding, NADP binding, NADPH-hemoprotein reductase activity.7. The protein should be designed to have high stability and activity towards NADPH + 2 oxidized [cytochrome P450] = H(+) + NADP(+) + 2   reduced [cytochrome P450]. The designed protein sequence is ",
    "response#1": "MIGPGTGVAPFRAFVQERAAQAKAGENVGRTILFFGCRKSTEDFMYKEEWKEYEEALGDKFSLITAFSREGKEKVYVQHRLKEHAKEVNDLLMQKAYFYVCGDAANMARELEDFLYKDEWPEDAKRPYNAPIVTREVFQDGGDRHCLHVDIDISGTNLTYQTGDHVAVWPTNNDVEVHRLASVLGLSEKLDTVVLVKAVDPAASKQYPFPAPTTYRAIFRHYIDVCAIPSRQALTSLVDYAPTEESKATLLKLASDKDEYRHAVSDAVRNLGEVLQYVMGDTSASATTSASSFASVPFDLIVECVSRLQPRYYSISSSSKEHPTLISATAVTLEYKPDASLERTVYGVNTNPFDHTHPFLARIVKTKELFTSEDRHCVHVEFDISESNLKYTTGDHLAIWPSNSDENIKQFAKCFGLEDKLDTVIELKALDSTYSIPFPNPITYGAVIRHHLEISGPVSRQFFLSIAGFAPDEETKKSFTRIGGDKQEFASKVTRRKFNIADALLFASNNRPWSDVPFEFLIENVQHLTPRYYSISSSSLSEKQTINVTAVVEAEEEADGRPVTGVVTPYNAHNPYIAPIAESKELFTVQDRNCLHLEVDISGSNLSYQTGDHIAIWPTNAGREVDRFLDVTGLSSKKDSVIAVKPLESTAKVPFPSPTTYDAIVRYHMEICAPVSRQFLATLSAFAPNDAIKAEMEKLGGDKDYFHQKITKNYLNIAQVLQEVGGQEKWTKIPFSAFIEGLGKIQPRYYSISSSSLVQQKKISITAVVESIDIPGRTDALKGVTTNPYNAANPYIAPIVESKELFHDPQRNCLHMEIDIGNTNLKYTTGDHIAVWPTNAGMEVDRFLKVLGLYEKRNNVITVKGTDPTARVPFPSPTTYDAIVRYHMEICAPVSRQFVSQIAPFAPTEEIKKSMTALGSDKDLFHKEVADWSLNIAQLLETQSKGTAWPAAPFSLFIEGLNKIQPRYYSISSSSHAQPTKVAITAVVESMHKPKAPHVLKGVTTN",
    "response#2": "MIGPGTGVAPFRGFIQERIALARRSIAKNGPDALADWAPIYLFYGSRDEQDFLYAEEWPAYEAELQGKFKIHVAFSRSGPRKPDGSKIYVQDLLWDQKEVIKSAIVEKRASVYICGDGRNMSKDVDAKRPYNAPIVTREVFQDGGDRHCLHVDIDISGTNLTYQTGDHVAVWPTNNDVEVHRLASVLGLSEKLDTVVLVKAVDPAASKQYPFPAPTTYRAIFRHYIDVCAIPSRQALTSLVDYAPTEESKATLLKLASDKDEYRHAVSDAVRNLGEVLQYVMGDTSASATTSASSFASVPFDLIVECVSRLQPRYYSISSSSKEHPTLISATAVTLEYKPDASLERTVYGVNTNPYNAANPYIAPIVESKELFHDPQRNCLHMEIDIGNTNLKYTTGDHIAVWPTNAGMEVDRFLKVLGLYEKRNNVITVKGTDPTARVPFPSPTTYDAIVRYHMEICAPVSRQFVSQIAPFAPTEEIKKSMTALGSDKDLFHKEVADWSLNIAQLLETQSKGTAWPAAPFSLFIEGLNKIQPRYYSISSSSHAQPTKVAITAVVESMHKPKAPHVLKGVTTNPYNAHNPYIAPIAESKELFTVQDRNCLHLEVDISGSNLSYQTGDHIAIWPTNAGREVDRFLDVTGLSSKKDSVIAVKPLESTAKVPFPSPTTYDAIVRYHMEICAPVSRQFLATLSAFAPNDAIKAEMEKLGGDKDYFHQKITKNYLNIAQVLQEVGGQEKWTKIPFSAFIEGLGKIQPRYYSISSSSLVQQKKISITAVVESIDIPGRTDALKGVTTN",
    "response#3": "MIGPGTGLAPFRGFLQERLALKEAGTELGSSVLFFGCRNRKLDFIYENELNNFVESGALSELILAFSREGPTKEYVQHKMNEKALEIWNFLSEGAYLYVCGDAKGMAKDVDAKRPYNAPIVTREVFQDGGDRHCLHVDIDISGTNLTYQTGDHVAVWPTNNDVEVHRLASVLGLSEKLDTVVLVKAVDPAASKQYPFPAPTTYRAIFRHYIDVCAIPSRQALTSLVDYAPTEESKATLLKLASDKDEYRHAVSDAVRNLGEVLQYVMGDTSASATTSASSFASVPFDLIVECVSRLQPRYYSISSSSKEHPTLISATAVTLEYKPDASLERTVYGVNTNPYNAHNPYIAPIAESKELFTVQDRNCLHLEVDISGSNLSYQTGDHIAIWPTNAGREVDRFLDVTGLSSKKDSVIAVKPLESTAKVPFPSPTTYDAIVRYHMEICAPVSRQFLATLSAFAPNDAIKAEMEKLGGDKDYFHQKITKNYLNIAQVLQEVGGQEKWTKIPFSAFIEGLGKIQPRYYSISSSSLVQQKKISITAVVESIDIPGRTDALKGVTTNPYNAHNPYIAPIAESKELFTVQDRNCLHLEVDISGSNLSYQTGDHIAIWPTNAGREVDRFLDVTGLSSKKDSVIAVKPLESTAKVPFPSPTTYDAIVRYHMEICAPVSRQFLATLSAFAPNDAIKAEMEKLGGDKDYFHQKITKNYLNIAQVLQEVGGQEKWTKIPFSAFIEGLGKIQPRYYSISSSSLVQQKKISITAVVESIDIPGRTDALKGVTTNPYNAANPYIAPIVESKELFHDPQRNCLHMEIDIGNTNLKYTTGDHIAVWPTNAGMEVDRFLKVLGLYEKRNNVITVKGTDPTARVPFPSPTTYDAIVRYHMEICAPVSRQFVSQIAPFAPTEEIKKSMTALGSDKDLFHKEVADWSLNIAQLLETQSKGTAWPAAPFSLFIEGLNKIQPRYYSISSSSHAQPTKVAITAVVESMHKPKAPHVLKGVTTN"
  },
  {
    "reference": "MQVSVEATQGLERRLTISVPAEQIEKLVKDSLQREAKRARIPGFRPGKVPVTVINKRYGAAIRQDITGEVMQRNFIEAIIAEKLNPAGAPTFVPGATDGEKFEFVATFEIYPEVELKGLDAIVVEQPKASVTDADVDSMIETLRKQHATFAAVEREAVDGDKVKMNFVGSVDGVEFEGGKAEDFELQLGSGRMIPGFEAGILGHKAGEEFVIDVTFPEEYHAENLKGKAAKFAITLTEVLAANLPEVNDEFAALFGITEGGLEALKTEIRKNMNRELEQALKANVKEQVIAGLLANNDIELPKALIDGEVNVLRQQAMQRFGGQTANMPELPAELFTEQAARRVKIGLLLGEVIKTNELKAEDERVQGLIASMASAYEDPSEVIAYYNSNKELMQNMRNVALEEQAVEALLKTAKVTEKTVAFEEFMNKATGRA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The desired protein should be able to perform [protein]-peptidylproline (omega=180) = [protein]-   peptidylproline (omega=0)2. A protein that contributes to cell division, chaperone-mediated protein folding, cell cycle, protein transport by functioning in cytoplasm with protein folding chaperone, ribosome binding, peptidyl-prolyl cis-trans isomerase activity.3. The protein must exhibit the following characteristics: Functions as a peptidyl-prolyl cis-trans isomerase.; Involved in protein export; Acts as a chaperone by maintaining the newly synthesized protein in an open conformation.4. The designed protein have PPIase FKBP-type domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "TLDELKAKYRKELEASKEVAFDDAVESAALELAVENAEIVELPEEMIHEEVHRAINEFLGGMQQQGISPDMYFQITGTTREDLHKQYEADAEKRTKTNLVVEAVAKAEGFEATEEEINKEIEDLAATYNMEVAQVRSLLSPEMLKHDIAVKKAVEVIE",
    "response#2": "MTAKWEKLEGNEGVLTVDVESAKVDEALDKAFKKVVKKVNVPGFRKGKVPRKIFERQFGVEALYQDALDILLPEAYAAAIDETGIEPVDRPEIDIEQMEQGNNLIFKATVTVKPEVQLGDYKGLEFEEKDTTVSDEDVEQELKSMEKAQARNRA",
    "response#3": "MGASLKKIEKSVATLELTIPKEKFEEGLDYAFKKNASKFNVPGFRKGKAPRFLVERYYGEGVLYEDAIEYVFHEAYQEALKTFNLEPVDYPDINILQIGKGKDLVLEATVAVMPEVELGEYKGIEIEKIEYDVYDGDVEYELEKLKAQEKRAAQGDVVVVDFVGKVDGVAFEGGTAQDVNVELGGAGFIPGFAEQIEGMSPGEEKVITVTFPADYSAENLAGKEATFDIRVLEQVRELITRQVEQEYEQLSRLRIKRELLDALAEKTDFEAPQGMVEAEFAQIWQRVEADRKAGELDEEDKEKDEDTLRADYRKIAERRVKLGLLLAEIGRANAITVSQDEMLQAIRAEAMRYPGQEQQVFEFFRKNPQAAESLRGPIFENKVVDYVIEVRSAA"
  },
  {
    "reference": "MQRLYGKTLAELGEIAQALSFPSYTAKQIAQWLYQHHINHIDEMNNLSKTARDKLKERFTLGLSAPVRVQISKDGTKKYLYSLAGGRFIEAAYIPDNDRGTLCVSSQMGCKMGCLFCMTGKQGFQANLTVNEILNQIRCLPEWHELTNIVFMGMGEPLDNIDNLMKSLEILTSDWGYARSPKRITVSTIGIIPTMKTFLENSHCHLAVSLHSPFNEERKKLMPVENVYPINEIVQLLKNYDWTGQRRLSFEYIMFKGLNDSLTHARALLKLLNGLNCRLNLICFHAIPGSTLEPSTNETIHSFKDFLNSKNLLTTIRASRGEDIFAACGLLSTKELIQQKNIGNSIGD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that can perform 4 iron, 4 sulfur cluster binding, rRNA binding, tRNA (adenine-C2-)-methyltransferase activity, tRNA binding, metal ion binding in cytoplasm to support rRNA base methylation.2. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.3. The S-methylcysteine intermediate, Proton acceptor of the protein must be engineered to enable effective catalysis of adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.4. The protein must be engineered to have a high affinity for [4Fe-4S] cluster in order to increase its catalytic activity.5. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity.6. The protein should have a high affinity for S-adenosyl-L-methionine, [4Fe-4S] cluster. The designed protein sequence is ",
    "response#1": "HGARNAVCVSTQAGCPMACAFCATGEMGLVRNLTAGEIVDQVRHWQRELAQRGERVSHVVFMGMGEPFNNYDATLASTRALIDPEAFGISPRRLTISTCGVVTGIDQLAGEGLPINLAVSLHAPDDPTRARIMPINRKWGVEEVLAAAARYVKRTKRRVTFEYVLLAGVNDSEETARELAALITRHGPTKDAHVNLIPYNPGAGGFRRPSVERMERFAALLQEHGIAATLRISKGQDIDAACGQLRRR",
    "response#2": "EGLGEPSYRGGQILEWLWQKRATSIEGMSNLPVRLREHLEESFTLRSLSPAKSQGSRDSTRKFLFRLNDGRYIECVLIPASPALYGERSDRRTLCVSSQVGCAFGCKFCASGLDGFTRNLTADEIAGQVLLAEELSGEKVNNIVFMGMGEPLANLPQLLKALELITSPWGLGIGARHLTVSTSGLVPQIRKLADHPQQIRLAISLHGASNDVRDRIMPVNRKWPVEDLFKALSYWTKRKNQKITLEYILIDGINADPAQARLLAQHAESLRAKVNLIPYNTVDGLKWKRPPEEECHAFRDILAARGIPATLRLEKGHDIDAACGQLRLKEERAEKDRARGR",
    "response#3": "DRDIKSLTREELREALLARGEKAFRADQVYSWMHERLASGFCEMTNLSKALREALEEEYDYTNLEIVEVQRSRLDGTCKYLFRLADGNVIESVLMRYHHGNSVCISSQAGCRMGCRFCASTIGGLVRNLKASEMLDQIYRIQKDTGERVSNVVVMGTGEPLDNYENLLRFIRILSEEHGLHISQRNITVSTCGIAPRIRELADEDLSITLALSLHAATQEKREELMPIARKYQLPEVLEACAYYFKKTGRRLTFEYSLVGGVNDHPEDARRLAELARPVHSHVNLIPVNPVTERSFVQPDKKVIGNFKKELEKYGINVTIRREMGRDIDGACGQLRRRYLERRG"
  },
  {
    "reference": "AXEAIFGAVVXIDL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must possess ATP binding to support negative regulation of hematopoietic stem cell differentiation, iron-sulfur cluster assembly under certain conditions. The designed protein sequence is ",
    "response#1": "LYTLRDLNVDVAIMHGPPGCSFKHARLLEEDGIHVVTTGLDENNFVFGGHDKLVQLINKSVELFNPKLIGIVGTCPSMIIGEEMHDAVLEANPDVPVIEVEVHAGYHDNTKGVLFALESALDC",
    "response#2": "LYTLRDLNVDVAIMHGPPGCSFKHARLLEEDGIHVVTTGLDENNFVFGGHDKLVQLINKSVELFNPKLIGIVGTCPSMIIGEEMHDAVLEANPDVPVIEVEVHAGYHDNTKGVLFALESALDC",
    "response#3": "LYTLRDLNVDVAIMHGPPGCSFKHARLLEEDGIHVVTTGLDENNFVFGGHDKLVQLINKSVELFNPKLIGIVGTCPSMIIGEEMHDAVLEANPDVPVIEVEVHAGYHDNTKGVLFALESALDA"
  },
  {
    "reference": "MSRTEASGWGFSLLQILLVLAGMVWKAREGFPIVDFLDTSGDTCSWLNPCIVPPALSCWNNWPWFALHLFLYTTQLVGLSAIILPPVYLIRMLGLSTALPLISLWVLHRRRRTGKGWATTFPPRHGEEAFLWRVLWFTGLAHVASFLVATAASFLGREDMAPWHLTNTLLGTPDCSYLPCSQIAERQARLRQINEMTGTSSGFFLAVALFSQHLEMNGARLGAGLLARLFFVSLIAGPAAGAADALLLRSAFMRTRRST",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a specific function within the membrane.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. The protein must exhibit the following characteristics: The cytochrome P450 monooxygenase idtQ may catalyze the C-13 oxidation of 13-desoxypaxilline to afford paxilline (Probable); The geranylgeranyl diphosphate (GGPP) synthase idtG is proposed to catalyze the first step in IDT biosynthesis via catalysis of a series of iterative condensations of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), geranyl diphosphate (GPP), and farnesyl diphosphate (FPP), to form GGPP (Probable); Part of the gene cluster that mediates the biosynthesis of paspalitrems, indole-diterpene (IDT) mycotoxins that are potent tremorgens in mammals. The designed protein sequence is ",
    "response#1": "KNDLLLRFSKIDYNIVQMLHKQELCHISKWWRDSELETKLTYARNRVAECFLWTLCVYHEPKYSPARLLLGKLINIISCTDDTYDAYGTLEEVQIFTDVIQRLDRSSMEQLPDYMKILYKAVLDLFDEVEVQLSNQETNNTYRMAYAKEELKAIAKCYEKEHIWFRKCHVPPFEEYLENAVVSIGNRLAVTFSFLGMDQVAAVEAFEWAKTDPKMVKSCGKVLRLVDDVMSHEEEDVRGHVATGVECYMKEHGVSREEAVVEFYKRVEYAWKDVNEEFITPNHLHIDLLNRVLNLTRIADVVYKFEDGYTHPEKTLKHHIMALFVDPE",
    "response#2": "KNDLLLRFSKIDYNIVQMLHKQELCHISKWWRDSELETKLTYARNRVAECFLWTLCVYHEPKYSPARLLLGKLINIISCTDDTYDAYGTLEEVQIFTDVIQRLDRSSMEQLPDYMKILYKAVLDLFDEVEVQLSNQETNNTYRMAYAKEELKAIAKCYEKEHIWFRKCHVPPFEEYLENAVVSIGNRLAVTFSFLGMDQVAAVEAFEWAKTDPKMVKSCGKVLRLVDDVMSHEEEDVRGHVATGVECYMKEHGVSREEAVVEFYKRVEYAWKDVNEEFITPNHLHIDLLNRVLNLTRIADVVYKFEDGYTHPEKTLKHHIMALFVDPA",
    "response#3": "KNDLLLRFSKIDYNIVQMLHKQELCHISKWWRDSELETKLTYARNRVAECFLWTLCVYHEPKYSPARLLLGKLINIISCTDDTYDAYGTLEEVQIFTDVIQRLDRSSMEQLPDYMKILYKAVLDLFDEVEVQLSNQETNNTYRMAYAKEELKAIAKCYEKEHIWFRKCHVPPFEEYLENAVVSIGNRLAVTFSFLGMDQVAAVEAFEWAKTDPKMVKSCGKVLRLVDDVMSHEEEDVRGHVATGVECYMKEHGVSREEAVVEFYKRVEYAWKDVNEEFITPNHLHIDLLNRVLNLTRIADVVYKFEDGYTHPEKTLKHHIMALFVDPS"
  },
  {
    "reference": "MVAEPRVTIIGATGAVGKVALEVLEQRNFPMSSLRLTASERSIGKKIDFRGQSITVEATTENILSESDIAFISANDDISRQLAPPAKEHNCVVIDDSGVWRMDPEVPLVVPEINATDIVNHKGVISIPNCSTTPLAMVLDALRPEAKIVSVIVSTYQSVSGTGAAAVAELSQQAKSVLNNXPIEVEQYPYQIAFNLLPHIGSFNQNSYTSEETKMLNETRKILHSPTLPVSATCVRVPVNICHSESVNIQFEKAITAERARQLLSNYEGVIVVDDPTENKYPTPIDCAGRDEVFVGRIRQDTALENGLSLWIVSDNLRKGAATNAIQIAEELVKLNAW",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that can modulate the overall flux of metabolites through the L-threonine from L-aspartate: step 2/5 pathway, enhancing or suppressing its activity as needed.2. The protein with the reaction L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH, the Proton acceptor must be optimized to ensure efficient catalysis.3. The protein design should be able to enhance the efficiency of 'de novo' L-methionine biosynthetic process, threonine biosynthetic process. The designed protein sequence is ",
    "response#1": "LKPLMKFGIEKIYLASMQAISGAGYDGVASMAILDNVIPYIDKEEEKVETETKKLLGEFNGSEIVDAPFAVSASCNRVMVMDGHTEAVWVEMADNPSTEDIKQAFLEFDPGLSDLPTEPAPVIEVKEEKDRPQPRMDRNLGGGMTVSVGRIRPGIRYICMGHNR",
    "response#2": "LKPLMKFGIEKIYLASMQAISGAGYDGVASMAILDNVIPYIDKEEEKVETETKKLLGEFNGSEIVDAPFAVSASCNRVMVMDGHTEAVWVEMADNPSTEDIKQAFLEFDPGLSDLPTEPAPVIEVKEEKDRPQPRMDRNLGGGMTVSVGRIRPGIRYICMGHNI",
    "response#3": "LKPLMKFGIEKIYLASMQAISGAGYDGVASMAILDNVIPYIDKEEEKVETETKKLLGEFNGSEIVDAPFAVSASCNRVMVMDGHTEAVWVEMADNPSTEDIKQAFLEFDPGLSDLPTEPAPVIEVKEEKDRPQPRMDRNLGGGMTVSVGRIRPGIRYICMGHNK"
  },
  {
    "reference": "MELGWLLWGAAVLLLLHVVMQLSPSINFALRIGFYSFLCLLASALTAPVCLLVNGGRTVKNMRIIRSVVRSFKYFFGLRFEVKGLENFKVEGPAIIVSNHQSILDMMGLMEVLPDDCVQVAKKELIYAGSVGLIIYLGGVIFINRKSTTSAKMVMAEVAKSMIAENVKVWVYPEGTRNCTGDLLPFKKGAFHLAVQAQVPVIPVVYSSFTNFYDPKKKLFTSGKIKVEILPPMETKGLTSDDVSDLSDRCFHTMRETLFKLSG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should contain a Helical membrane-spanning region.2. The designed protein should contain one or more Phospholipid/glycerol acyltransferase domains that are essential for its function.3. Design the protein with a signal peptide incorporated.4. The desired protein should be able to perform a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-   sn-glycero-3-phosphate + CoA5. The designed protein should have a specific function within the membrane.6. The protein must exhibit the following characteristics: Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.. The designed protein sequence is ",
    "response#1": "MQVRGSEHLNLQEPFVIVCNHQASLDLLGMVEVIPERCVPIAKKELKYMGTVGWACWLSGIIFIDRFRREVAIDVISKTAETVRRENLRVWIFPEGTRNQSKSMLPFKRGAFHLAVQAQVPILPIVIGIKMQVRGSEHLNLQEPFVIVCNHQASLDLLGMVEVIPERCVPIAKKELKYMGTVGWACWLSGIIFIDRFRREVAIDVISKTAETVRRENLRVWIFPEGTRNQSKSMLPFKRGAFHLAVQAQVPILPIVIFSSYSSFYSFTKIQVRGSEHLDVKEPYVIVCNHQASLDLVGMVEIIPDHCVPIVKKELLYMGTVGWACWLCRMIFINRHKREDAINVISRTARTIRRENLRVLIFPEGTRNQEKSMLPFKRGAFHLAVQAQVPIFPVVMFSSYSNFYSHKESKFRSGTLVVSVLDAVPTEGLTDLCNQVQVAMTKAFREMSQIGG",
    "response#2": "RMRVRGSEQLQLRGPFVIVCNHQASLDLMGMVEVIPERCVPMAKRELQFLGTVGWACWLSGTIFIDRRRRDAAIEVIARTARALRRDNLRVWVFPEGTRNPGKSMLPFKRGAFHLAVQAQVPIVPVVIFDIKGRENLEVDRPCVIISNHQSILDMMGLMEILPQRCVQIAKRELLFLGPVGLVMYLGGVFFINRQRSSTALTVMADLGERMVEENLKVWIYPEGTRNDNGDLLPFKKGAFHLAVQAQVPIVPVVYTSFGGDSQDLFNPKRVTFSVVPKVEAVGLSKEDVPELTEKLRASMVQVFSQL",
    "response#3": "MQVWGTEHLDLEEPYVIVCNHQASLDLLGMVEVIPERCVPIAKKELLYMGTVGWACWLSGIIFIDRHRREAAIDVISQTARTMWRENLRVWVFPEGTRNQNKSMLPFKRGAFHLAVQAQVPIVPIVIGIKYTIQGSENLKAKEPYVIVANHKSCLDILGMMEILPERCVAIVKKELLYTVVVGWPCWLCNFIFIDRKKKHAAMSTIEHLAHTIREQNLKIWVFPEGTRNPAHTLLPFKRGAFHLAVQAQVPIVPVVFSSYSNFYLRKEKKFKAGKIEVEVLPAIPTEGLTSDDVPKLTEKVRDSMISCFKNMSKE"
  },
  {
    "reference": "MKVAIVGVSGAVGQEFLRVLEQRNFPLDELVLFGSARSAGKEYEFRGKKIKVKELKHGDDFKGIDIALASAGAGVSKEFAATITKHGTIMIDNSSAFRYDDDVPLVVPEVNPGDARNRPRNIIANPNCSTIQMVVSLKPIDDLSKIKRVRVATYQAASGAGAEGIAELEQQVKEIAEGKKPTISTFPYQLAMNIIPHIDVFLDNDYTKEEMKMNWETKKIMHTDADVSATCVRIPVARAHSEAIWVETEEPLSVEQVKKAFANAKGLVVLDNPAEKQYPMPLFVSGEDDVYVGRIRKDITDPNTITFWCVGDQIKKGAALNTVQIAEWLIKEGEV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. Ideal protein should have a binding affinity for key enzymes in the Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5 pathway to enhance the overall efficiency of the pathway.2. The designed protein should be optimized for NADP binding, aspartate-semialdehyde dehydrogenase activity, NAD binding activity in order to improve 'de novo' L-methionine biosynthetic process, lysine biosynthetic process via diaminopimelate, diaminopimelate biosynthetic process.3. The designed protein must have a Proton acceptor, Acyl-thioester intermediate active site that is highly conserved among related enzymes.4. The designed protein should have a high specificity for NADP(+), substrate binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MKIAIVGATGAVGEEILNVLDELDFPVESILPLASAKSAGSEVEFKGKFYKVKELTDTVFKENCVDIAFFSAGGSVSEKYAKFAVQAGALVIDNTSHFRMQEDVPLVVPECNSEDIKNAQDKIIANPNCSTIQMVMVLAPLHEKYGIERVVVSTYQSITGTGVKAVRQLENEYQGIQGEMAYPYPIHRNALPHCDVFEDNGYTKEEMKLVRETRKILRDDSIAVTATAIRIPVVGGHSESVNLQLKNDFDLEDIRKILNDTPGVTVQDNTDTNTYPMPLYAHGKDDVFVGRIRRDFSQPNSLNMWIVADNLRKGAATNAVQIAEYLVENNLV",
    "response#2": "INQMFQIKSVFVSTYQSVSGAGKGGIEELKDQVAKLFSGQSTNPAVFPQRIAFNLIPQIGPFYPEGYCEEEVKMINETRKILSMNDLDIDVTTVRVPTFFSHAETVVIETENDCDINSVRKRLNDHPDIILMDDPEELLYPTLIDCSGKNEGFIGRLRMSKDNNKRISAWIVADNVRAGKV",
    "response#3": "MDLKPLHDAARITRINVATYQAVSGTGKEAIDELASQTASLLSGRPVECKVYPKQISFNALPHIDVFLENGYTREEMKMVWETHKIMESPDIQVNPTAVRIPVFFGHSEAVHIETGEKLSAAQAREILEKADGIVVMDERVEGGYPTAVTEGANSDPVYVGRIREDISHRSGLNLWVVADNVRDLEPLRRDYGLSRIVVSTYQAVSGAGVNAIDEMYEQTQQLLNEEEIEPKVLPVSGDKKHYQIAFNAVPQIDVFQENGFTYEEMKMINETKKIMEDESIKVAATCVRLPVETGHSESVYVEVEKEGLDVAKVRSLLEEAPGVTLQDDPSNQVYPLASEAKGKDPVFVGRIRQDIDEPKGFHMWIVADNLLGFPVLVAGLEPLRRDYGLSRIVVSTYQAVSGAGVNAIDEMYEQTQQLLNEEEIEPKVLPVSGDKKHYQIAFNAVPQIDVFQENGFTYEEMKMINETKKIMEDESIKVAATCVRLPVETGHSESVYVEVEKEGLDVAKVRSLLEEAPGVTLQDDPSNQVYPLASEAKGKDPVFVGRIRQDIDEPKGFHMWIVADNLLSIPADAPKDSDGIINQMFQIKSVFVSTYQSVSGAGKGGIEELKDQVAKLFSGQSTNPAVFPQRIAFNLIPQIGPFYPEGYCEEEVKMINETRKILSMNDLDIDVTTVRVPTFFSHAETVVIETENDCDINSVRKRLNDHPDIILMDDPEELLYPTLIDCSGKNEGFIGRLRMSKDNNKRISAWIVADNVR"
  },
  {
    "reference": "CSFSGEPAPADLCVMTQLLGYVGPSEPHSVAAVPTIAFYPLFWNVVAKWEHKTREPSGAFGSPHLACFTLGEAILLLNMLRSTQAMLSQPRMQSLDNPLAYRVGLALLGVGGVFVLSSFLALGFTGTSLGDYFRILKEARVTTFPFSVLDNPMYWGNTANHLGWGVFWGGIPSLRLLSSALFFGSQNGLPIYLSLHKGPWS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should interact with enzymes and substrates within the Phospholipid metabolism pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "IVYVPCHRSHMDYLLLSYILYYQGMVPPHIAAGINLNFWPAGPMFRRGGAFFIRRSFRGNKLYTAVFREYLDQLFAKGYSVEYFTEGGRSRTGRLLAPKTGMIAMTLNSVLRGVERPVTLVPVYLGYG",
    "response#2": "IVYVPCHRSHMDYLLLSYILYYQGMVPPHIAAGINLNFWPAGPMFRRGGAFFIRRSFRGNKLYTAVFREYLDQLFAKGYSVEYFTEGGRSRTGRLLAPKTGMIAMTLNSVLRGVERPVTLVPVYLGYG",
    "response#3": "IVYVPCHRSHMDYLLLSYILYYQGMVPPHIAAGINLNFWPAGPMFRRGGAFFIRRSFRGNKLYTAVFREYLDQLFAKGYSVEYFTEGGRSRTGRLLAPKTGMIAMTLNSVLRGVERPVTLVPVYLGYG"
  },
  {
    "reference": "MQAPPLYTAEQVRHLDQVAIESFGIDGFDLMKRAGKAAFRVARRHWPDARSMVVLCGGGNNGGDGYVVAGLAKQQGWDSRCIAVSDPEKLKGTARQALDFAGEAGVDVERYDALTPDSFNEALAGTELIVDTLLGTGLAGEVREPYASVIESVNHSGVPVLAVDIPSGLCSNTGRALGSTIIANATVTFIGRKAGLYTGKARDHTGPVIFDDLAVDPAVYDAEPEPAARLWSREVIRAALPGRRPTTHKGDCGRVLVVGGEQGMGGAGIMAAEAALRAGAGLVFLATRDAHVPVALARRPEVQARSVEHGNELDALLEKVDVVVLGPGLGRDAWGQQMFQRVCSFEGPVVVDADALNLLAEAGEKKHPNWVLTPHPGEAGRLLGTTVPEIESDRYQALKNLVDQHGAAVVLKGAGSLVGAPGQVPALIDAGNPGMATAGMGDVLSGVIGALLGQGLDGFSAAAAGAGWHALAGDEAARHVGMTGLLASDLCDYMFVAVADHPAETPDD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have a binding site that is specific to the target (6S)-NADPHX ligand.2. The designed protein should contain one or more YjeF N-terminal domains that are essential for its function.3. The protein must exhibit ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, NADHX epimerase activity.4. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.5. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. The designed protein sequence is ",
    "response#1": "MQKADKHTIEKIGISSMVLMERAALKTVEVMETEQIRLDKALIVCGSGNNGGDGFAIARLMKEKGYLPTVVFAGREQSLSMECRTQKEVLERLGLMSCTEIPSKEYTVVIDALFGVGLNRDIQGHYVDIIAQMNQISAKKVAVDIPSGIHAEKGCVMGNAFKADLTVAFACEKLGCVLYPGKEYSGKVLPVEIGIDLAIFLHRAQMQKLLPKRAGNAHKGSVGRVLVVGGYLSYTGAPSLAALGAYRTGAGLVTVAYPADCAVQPPLEAVRLPLLEWNTANLQRGRAEAVAVGMGAGDGGVQAALAALGLKRPTVLDADALYERVLQAYSEAALPTVITPHPGEASRLLGLPSEQVARFPLEAAQTLAKRYPGLVVVLKGGPTVLAWQPQGVPTSTPQLAVNSTGNPHMATGGMGDVLSGVIAALLAAGLSPWEAARLGVYLHGLAGDLAGKPGLLAHEVAEALPVAHRAQMQKLLPKRAGNAHKGSVGRVLVVGGYLSYTGAPSLAALGAYRTGAGLVTVAYPADCAVQPPLEAVRLPLLEWNTANLQRGRAEAVAVGMGAGDGGVQAALAALGLKRPTVLDADALYERVLQAYSEAALPTVITPHPGEASRLLGLPSEQVARFPLEAAQTLAKRYPGLVVVLKGGPTVLAWQPQGVPTSTPQLAVNSTGNPHMATGGMGDVLSGVIAALLAAGLSPWEAARLGVYLHGLAGDLAGKPGLLAHEVAEALPVAIEQLQAQLLAAEALPPRARYANKGNNGHVLVIGGEHGMAGAVRLAGESALRAGAGLVSVATRIGHVTALNARRPELMAHEVDGPQTLEPLLERASVLALGPGLGQAAWGHALWLTALDANKPLVLDADGLNLLARETRTFTAPTVLTPHPGEAARLLGVTTKDVERDRFAAVRELARRYKAVVVLKGSGSLIADPDGRLDVCPWGNPGMASGGMGDLLTGIVAALLAQGCSPWRAACLGVGLHARAGDFAAQQGERGLLASDLLEPLRLLGNGIS",
    "response#2": "MRAIEQAAIASGEVTGLELMERAGQGVVDAIFEEWPELAEPVEGGRGAGPSRSPEYLEKDEEPRRAVVLCGPGNNGGDGFVVARLLKERGWEVEVFLYGDPDKLPPDAKVNYERWRELGEVADLTAEAVGKGPRPVLLLDAMFGTGLSRAIPLELANAHHCVEMRDVGGVHRWRVPYYRVALDCPSGLDCDSGRFLLPEAPTETGNEEKDLEEKWSWWENDASRRLLRSDLTVTFHRRKIAHGLDGGLGKVVVADIGLGKEDQLVGSDSATLPPRRHAQHKGSFGDVAIVGGAPGMTGAALLAARAAHAAGAGRVYVSLLDGGSLSHDPLRPELMFRDGWWRGSESMLVKATVVCGCGGGIAVREPLARLLSTVGRLVLDADALNLIAQDSALQRLLQSRHARQRATVLTPHPLEAARLLGCDAAQVQGDRIDAARRLADRFACVVLLKGSGSVIAAPGEVPRINSTGNAALASAGTGDVLAGWLGGRWAQRAPSDNAFRDAMNAACAAAYAHGAVADDASTQVLRADDLIERLHG",
    "response#3": "MRAIEQAAIASGEVTGLELMERAGQGVVDAIFEEWPELAEPVEGGRGAGPSRSPEYLEKDEEPRRAVVLCGPGNNGGDGFVVARLLKERGWEVEVFLYGDPDKLPPDAKVNYERWRELGEVADLTAEAVGKGPRPVLLLDAMFGTGLSRAIPLELANAHHCVEMRDVGGVHRWRVPYYRVALDCPSGLDCDSGRFLLPEAPTETGNEEKDLEEKWSWWENDASRRLLRSDLTVTFHRRKIAHGLDGGLGKVVVADIGLGKEDQVLTGEQLRKELPERRSFSHKGTYGHALVLAGSKEMTGAPVLSASAGLRVGCGLLTLAVPEPALPIVASKINEPVFWEWPAEEGYFARESYTLLKQRIASFDVVAIGPGLGTWEGGNNWLRKIINMVDVPLVLDADALNLLSENLSILREKKGQIILTPHPGEMGRLCGCTVAEIERDRVGYARSFAQDYEVYVVLKGTYTLIATPDGKVFINTMGSAALAKGGTGDVLTGMLAGMLAQKVAQDEAIEVGIQLAVYLHGIAGRICGEKSMYATLAGDVIDNIGSAIKTAHKGLFGHVLVAGGERGFSGAVQMAASAALRVGAGLVSLATRSEHAALLNLVRPELMVHGIDKRVDLAPLLKKSSVAAVGPGLGQTVWAQNLLAELFDTAMPLVVDADALNLLAKEPFKRDNWVLTPHPGEAARLLGITIKEIESDRFSSVKALQKKYGGVVVLKGAGTLIFDGSEMVINTTGNPGMASGGMGDVLSGVIAALLAQGLSLSQSARLGVWLHGKAGDIAAKEGERGLLASDLLRPL"
  },
  {
    "reference": "MKICSAAQSRFLDQTTIAGNHLSGLELMENAGRGTVALMLDYYGDCKERRVAVFAGPGNNGGDGFVVARHLHELGAEVSVFLLVNPDRVGGDAAQNLQRLPAEIVVRPLLDQEAVDSLSLQVDIIVDALLGTGLTREVTGLFARLIDRINRSGVPVVAVDIPSGLDADSGVLWGCCLKADLTATYGVAKTGLVQYPGADYVGRLEVVDIGIPAEVLERAAITSSYLTEEVATDLLPQRQNNAHKGSHGHILVLAGSRGKTGAAVLAAHGALRSGVGLVSLCVPEGLNTIFEGLCLEAMTIPLRGLAGHALTNSDFGEIVRAAVGKRCLVVGPGLGLAPGTVALVNQVVRELEQPLVLDADALNGLELAGLTGSGADRVRVLTPHPGEMARLTGLTTQEVQNRRLQVAGYFARQYGVVVVLKGAATVVAGPDGELAVNSTGNHGMGTGGMGDVLSGVIAGLLAQQVDPFQAACLAVYSHGRAGDLLMAQGSYGYLASELAAMLPRVWAELHSA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.2. The designed protein should have a binding site that is specific to the target AMP, K(+) ligand.3. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration4. The protein design must be able to perform NADPHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, metal ion binding, ATP binding in order to contribute to nicotinamide nucleotide metabolic process.5. The desired protein should be able to perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate6. The designed protein must have at least one YjeF N-terminal domain. The designed protein sequence is ",
    "response#1": "MIVAGSRNYVGAAYLAATAATRVGAGLVTVAMPESLVGAVASKAVEPTYLPLPESSPGVVSPEAAGLVLRNLSGYSALLVGCGLGQAQPTHDLVEQLLLAGADLPSTVVDADGLNALSRVTDWHRRLTSDAILTPHPGEMNRLTGDRTPDDRVRTATLFASGWHSVVVLKGAHTVIAHPDGDAVLSPFANPGLATAGTGDVLAGAIAGLLSQSLSRGDAAKLGVYIHGLAGERVRDDMGDTGMIASDLLPVPQDDKYTQGVVGIVAGSARYPGAAVLSTAGSVAATSGMTRYVGAAAPQVVTHRPEVVAVTGLADAGTVQAWVVGPGIGTDGDAETLVEQVLATELPVLVDADGLTVVARRPELLERAAPTLLTPHAGEFERLAGAPVGDDRLSAVRALAARSGSTVLLKGRITLVAAPDGRVLGNDAGGSAAATAGAGDVLSGIAGALLAAGTPT",
    "response#2": "MQIKAIDAYTISGEPISSIDFMERSAQQFAKKVMSCTNANRKIYVFAGPGNNGGDALAVSRIMLENGYSVVVYLFNPKGKLSENCSLNKDRLWRMEGVHFVEVRDSFDPPDLKKEDVVIDGLFGIGLSRPLAGGFLAVVHYLNQSSAHIISLDIPSGLLADGDLQADDEAIICADKTFTFQFPKLSFFFPESGRYVGDWDLLDIELDGEVLRRHVPSGESHKYARGVVGVSAGSDAYAGAAHLCVAGAQSGLAGMVRFVGTDALAARVVDRAPEVVHGRGRVQAWVVGPGAGDDVGDQLAACLDDGVPLVVDAGAIEHLPHDPQVPVLLTPHAGELARLLDVDRETVEADPWAHAATAADRWGTTVLLKGARTLVVAPRNGSGERRCSVNLSGTPWLGTAGSGDVLAGFAGSLLAGGLDPFDAGRLAAFLHGAAAVRANPGGPVAASEVAAALPTTVAAFLDRGDYRAALPLPSPDAHKGQRGSVLLVAGSRLYAGSAVLAAAGAMRMGAGYVFAAVPASVAPVMQAALPHVIAVAMQETAEGTLAASSVDRIMRLAAEVDAVVLGPGLTTHAEAVDVIRRLVELLPGPLVLDADGLNAFAEPGPGAILSRSGPTVITPHPGELARILGVSTGEVQDDRVAAAARIAGCQLACVLKGARTIVSTDGRSVVTLAGNPGMATAGSGDVLAGMIGTLLAQGLGPLEAGALGAYVHGRAGDLASAELTEVCLTAKDLPSYIPAAVKEE",
    "response#3": "MQIKAIDAYTISGEPISSIDFMERSAQQFAKKVMSCTNANRKIYVFAGPGNNGGDALAVSRIMLENGYSVVVYLFNPKGKLSENCSLNKDRLWRMEGVHFVEVRDSFDPPDLKKEDVVIDGLFGIGLSRPLAGGFLAVVHYLNQSSAHIISLDIPSGLLADGDLQADDEAIICADKTFTFQFPKLSFFFPESGRYVGDWDLLDIELPTDDKYTQGVTGVMAGSDTYPGAAVLCTGAAVAATSGMVRYAGTAAQQVLAQWPEVIATSTFTTTGRVQSWVVGPGLGTDETAAAALCFALEADVPVIVDADALTILAAHPELVDGRSTATVLTPHAGEFARLAGAPPGPDRVADTRRLADRLGVTVLLKGNVTVIAEPGSGAVYLNAAGASWAATAGSGDVLSGVIGALLAAGLPAGEAAAAAAFVHSRAAQLSALDPGPGPAPTSASRITSDWSRVALPRRPSDSHKGTFGKTMIVAGSRNYVGAAYLAATAATRVGAGLVTVAMPESLVGAVASKAVEPTYLPLPESSPGVVSPEAAGLVLRNLSGYSALLVGCGLGQAQPTHDLVEQLLLAGADLPSTVVDADGLNALSRVTDWHRRLTSDAILTPHPGEMNRLTGDRTPDDRVRTATLFASGWHSVVVLKGAHTVIAHPDGDAVLSPFANPGLATAGTGDVLAGAIAGLLSQSLSRGDAAKLGVYIHGLAGERVRDDMGDTGMIASDLLPVLPKVIRDLRGVSAGSDAYAGAAHLCVAGAQSGLAGMVRFVGTDALAARVVDRAPEVVHGRGRVQAWVVGPGAGDDVGDQLAACLDDGVPLVVDAGAIEHLPHDPQVPVLLTPHAGELARLLDVDRETVEADPWAHAATAADRWGTTVLLKGARTLVVAPRNGSGERRCSVNLSGTPWLGTAGSGDVLAGFAGSLLAGGLDPFDAGRLAAFLHGAAAVR"
  },
  {
    "reference": "MGSGKTTIGRLLARRLNKRFVDSDHAIEARTGATIPWIFEIEGEASFRRREADMIRELTAQRGIVLATGGGAVLDAASRAHLAERGTVIYLRAGIGSILQRTSHDKNRPLLQTADPRRKLEELLAQREPLYREIADLVIDTGRPNVQSMVQTILDQLDALNSARSRRARAASMNQTAHTNLNVELGERSYPIAIGPGVLGDSALLARQLAGTKVAIVTNTTVAPLYLDKVAAPLRAAGREVVEIVLPDGEEHKGWGSLMAVFDALLANKCDRKTTLVALGGGVIGDLTGFAAASYMRGVPFVQIPTTLLAQVDSSVGGKTGINHPLGKNMIGAFYQPRAVLADTSTLSTLPDRELSAGLAEVIKHGAILDAAFFDWIEANIARLVARDPQALSHAIARSCEIKADVVRKDEREGGLRAVLNFGHTFGHAIEAGLGYGTWLHGEAVGCGMVMAADLSRRLGLIDDASVERVRALVKAAGLPTVAPNLGVARWIELMEVDKKNEGGAIKFILLKPLGSPSIASVPREVLEATLEACIQH",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The ATP, NAD(+), substrate, Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.2. The protein design must be able to perform shikimate kinase activity, ATP binding, magnesium ion binding, 3-dehydroquinate synthase activity in order to contribute to chorismate biosynthetic process, amino acid biosynthetic process, phosphorylation, aromatic amino acid family biosynthetic process.3. The protein contains novel 3-dehydroquinate synthase domains that confer a unique function or activity.4. The protein should have a fast turnover rate for ATP + shikimate = 3-phosphoshikimate + ADP + H(+).5. The protein design should incorporate a binding pocket that specifically recognizes NAD(+) to enhance enzymatic efficiency.6. A protein that can modulate the overall flux of metabolites through the chorismate biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MLVPDAEAGKTIQVASGLWARLGDAGFTRSDAIVGLGGGAATDVAGFVAATWMRGIRYVNCPTSLLAMVDASTGGKTGINTDAGKNLVGSFYTPAGVLADTSTLSTLPNDLTRGISQVEFGYAEVAKHGLLSGEALWRWCEAEGAALVAGNPAALAHAVLESCRLKAAVVAADEREESPEGGRALLNLGHTFGHALEAECGYGGALLHGEAVAIGLGLAAALSARLGHCSQELPGRVMSHLAACGLPARLPDLSRRFSAAALLARMRKDKKGLGEVVKCGFIADPAILDLVEGVAPEEITADSPVLRELVERAIGVKIAVVVTDLKETGGVDGHPGREALNYGHTLGHAIERAEGYRIRHGEAVALGCVFVAELARRTGVLSADVAARHRSVLARVGLPTTYAGAGFEELLAAMRVDKKVAKRF",
    "response#2": "MLVPDAEAGKTIQVASGLWARLGDAGFTRSDAIVGLGGGAATDVAGFVAATWMRGIRYVNCPTSLLAMVDASTGGKTGINTDAGKNLVGSFYTPAGVLADTSTLSTLPNDVFIEDVEEKKDISTLSKIYDEFIKLGVDRKSLIIALGGGITGDVAGFAAATYMRGIKWVYVPTTLLAQVDASVGGKVAVNYREGKNLIGAFYQPDLVITDISHIFTLPDKIESAQIALGGYAEAIKEGFILDASLVDVFEEHAEALMDLEPEISTQVIRRSMAIKADVVSQDERETLGVRILLNYGHTIGHALEASTEYGRFMHGEGVSVGMMGAAHISQGMGLIPQSIVDQQERLLKRFNLPTTASGVPIDDIFRAMSLDKSVGRLFGYINKGLGEVVKCGFIADPAILDLVEGVAPEEITADSPVLRELVERAIGVKIAVVVTDLKETGGVDGHPGREALNYGHTLGHAIERAEGYRIRHGEAVALGCVFVAELARRTGVLSADVAARHRSVLARVGLPTTYAGAGFEELLAAMRVDKKL",
    "response#3": "MLVPDAEAGKTIQVASGLWARLGDAGFTRSDAIVGLGGGAATDVAGFVAATWMRGIRYVNCPTSLLAMVDASTGGKTGINTDAGKNLVGSFYTPAGVLADTSTLSTLPNDIHLCALNDDTITFGFAEVIKHALILDTELIDDLAEHAAELAGGTADPSLLAAVIGRSVRAKALVVSSDPHEAGMRAILNYGHTIGHGVEAAGGYEQFLHGEAVAVGMVGAARIAERMGLLDPGDSRRHEELLRAFGLPVACPGIDVEAVLQAMRYDKLFYPRLLRHNGLGEVAKSGFILDGEILKILSDSNNAKLLREFDGDQLENNNELHELLVELIERTVRVKAYHVSSDLKEQGLREFLNYGHTLGHAIEQIEHFRWRHGNAVAVGMVFAAELAQILGYLDTQDVSYHRSLLDSLGLPTSWQSREDADSAFERVLNLMHRDKKLEELGYPKPSIVVPDAEKGKTVAVASGVWKRLGDEGFTRSDAIVGVGGGAATDLAGFVAATWMRGIRYANCPTSLLAMVDASTGGKTGINTDEGKNLVGSFYTPAGVLADLRTLQSLPNDIQVCALNDPTITLQALDDETITFQALEDETISFQALEDETISFQALEDETFSFQALEDETISFQALEDETISFQALEDETISFQALEDETISFQALEDETISFQALEDETISFQALEDETISFQALEDETISFQALEDETISFQALEDETISFQ"
  },
  {
    "reference": "MKIVFATNNAHKLDEIREILGPEFEIASLNDIGCHEDIPETGDTLEANAMQKARYVARKYRLSCFADDTGLEVEALGGEPGVHSARYAEGTDHDSEANMQKLLRRLGDADNRRARFRTVIAYLPLTASGTAEGEPLLFEGTVNGRIDRAKHGTGGFGYDPLFIPDGYEHSFAELGMEVKNRISHRARAVQKLVDYLKTRQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design must be able to perform dITP diphosphatase activity in order to contribute to nucleotide metabolic process, purine nucleoside triphosphate catabolic process.2. The protein should have a high affinity for Mg(2+).3. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.4. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "MEIILATRNAKKLAELRGMLGGVPDLTIVGYDGPEPVEDADTFEANA",
    "response#2": "MKIVLASNNAKKLRELTDILGPAGFEIVAQGDLGVPEAEEPHCTFVENALAKARHAAALTGLPAIADDSGLCVDALDGAPGIYSARFSGEGATDAANNAKLLAELGGVPAERRTAHFHAVLVLVRHADDPEPLIAEGSWHGEILPAPRGTNGFGYDPHFFLPSLGKTAAELEPEEKNAISHRGQALRAMRERLAALG",
    "response#3": "MPIGVPKVSFRNPGDEVSTEQCHRIYQERR"
  },
  {
    "reference": "MSKIPVIVIVGPTAVGKTSLSITLAKNFDGEIISGDSMQVYRGLDIGTAKITPEEMDGIKHYLIDVTDPAVPFTAAKFQAETRGLIESIHNRGKLPIIVGGTGLYIQSVFYDYGFGNASEDKAYRRELDQLDKTTLWQMLDQLDPKSAELIHENNKRRVIRALEVIHLTGKPFSEYQVHHTLNEAYQPLFLGLDLDRELLYERINRRVELMFEEGLVSEASKLYDEHLVDVPAIRGIGYKELFTYFDGNSSLEEAKELIQKNSRHFAKRQLTWFRNRMDIDWIQAGVSTTDTEAMEKVKTFLASK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. A protein with tRNA dimethylallyltransferase activity is needed to enhance tRNA processing in a specific context.3. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate, ATP binding.4. The protein should be designed to catalyze the following reaction adenosine(37) in tRNA + dimethylallyl diphosphate =   diphosphate + N(6)-dimethylallyladenosine(37) in tRNA.5. For general function, the protein need meet that Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A). The designed protein sequence is ",
    "response#1": "KRLILVVGPTGTKKSYLANMLAKKLNVPIISADAYQVYKELNAGVNKPSEKTLSEIKYHFISNISIFDEWSIAHFNKRAKEILEQAPDWTIVCGGSHLYVNSLINDYQLEKQELDTDLFDALDKLDNQEIFNQLCEYDFQEAIKIGKNNRKRLLRALYLFKKYGKKAKNDSKKFDYVVVKCMSDKEKLFPYLSKRLL",
    "response#2": "MKTKRLILVVGPTGTKKSYLANMLAKKLNVPIISADAYQVYKELNAGVNKPSEKTLSEIKYHFISNISIFDEWSIAHFNKRAKEILEQAPDWTIVCGGSHLYVNSLINDYQLEKQELDTDLFDALDKLDNQEIFNQLCEYDFQEAIKIGKNNRKRLLRALYLFKKYGKKAKNDSKKFDYVVVKCMSDKEKLFPYLSKRLL",
    "response#3": "MKRLILVVGPTGTKKSYLANMLAKKLNVPIISADAYQVYKELNAGVNKPSEKTLSEIKYHFISNISIFDEWSIAHFNKRAKEILEQAPDWTIVCGGSHLYVNSLINDYQLEKQELDTDLFDALDKLDNQEIFNQLCEYDFQEAIKIGKNNRKRLLRALYLFKKYGKKAKNDSKKFDYVVVKCMSDKEKLFPYLSKRLVG"
  },
  {
    "reference": "MLILGCRQAVRQLVLVQLFEGSNPSTPTFLLYGTYMGITDEILILSGTANPQLSEDVVKNLGLKLGNMEIRKFADGETFVQIEETVRNKDTYVIQPTGRPSSSESWMELYCIIDALKRASAKRITAVIPYYGYSRQDRKNEPRVPITAKLVANLLSEAGAHRVLALDLHAAQIQGFFDIPVDHMLSKNVFLDKIRKDLDMSNSIIVSPDIGGVGRARAVAKQLNLDIAIIDKRRDRANECEVMNIIGDVNGKDAIIIDDIIDTGGTLIKSMQALKKAGMRKIYVFITHAVCSGDVYERINASDIEKLYITDSLKVMKDRLGSKIEVLSVAPVIADAIRHIHMELSISVLFDK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have a active site that is structurally stable and resistant to denaturation.2. The presence of Mg(2+) is necessary for the protein to function in the desired environment.3. The designed protein should have a high specificity for Mg(2+), ATP, D-ribose 5-phosphate binding, with minimal off-target effects.4. The designed protein should be involved in 5-phosphoribose 1-diphosphate biosynthetic process, ribonucleoside monophosphate biosynthetic process, nucleotide biosynthetic process, phosphorylation by localizing to cytoplasm and exhibiting ATP binding, magnesium ion binding, kinase activity.5. The protein should be optimized for activity in 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1 pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MKVFAGNANPALAMKIADYIGIPMGNLVIQRFTDGETFVKICDNIRGKDVFVVQPLCYPPNEMIMELLIMVDAMKRASAERITAVLPYYGYARQDRKDQPRVPITAKLVANLLVAAGDARRHLGENAPLLARYFVENKLDDDLVIVSPDHAGVSNARKLAELLHSPIAIIDNRDDNESTTSPDAVIGSVKGKRAILIDDIIDTGVRIANSAKTLERAGATEVFAVATHAVFSGNAVEELNNSPLKKVVFTDASPVLFEYLKTLDTDNLTLFSPDVGGMKMASAYAGMLQVPLGFIAKRRTDAETVEAVSLVGETDGRDILLIDDMTETAGTLCAAAELLKKNGANRVLASVSHGVLNNLGYERLSTGLLDRLITTDTITLPDQYRQAPLLADYFLSNDLAENAVVVSPDHGGVTRARKLADFLKTPIAIIDKRRPRPNVSEVMNIIGNVNGKRAIIIDDMIDTAGTITLAAQALKDAGATEVFACCTHPVLSGPAIQRIEDSVIKQMIVTDTITLPDQYRQHPQIQVLTVDQLLGEAIRRIHRNESVTSLFEH",
    "response#2": "ILSRYFKERDLSELTVLATDVGDAKRARNIARQLDTPLAVVDKERMGNEERVSALSLIGDVKGRHVIIPDDEIDTGGTILAAARIAIERGAKSVTTACVHPILSDRAAEKICASEIQELVVTDAPLLARYFVENKLDDDLVIVSPDHAGVSNARKLAELLHSPIAIIDNRDDNESTTSPDAVIGSVKGKRAILIDDIIDTGVRIANSAKTLERAGATEVFAVATHAVFSGNAVEELNNSPLKKVVFTDAPLLARYFVENKLDDDLVIVSPDHAGVSNARKLAELLHSPIAIIDNRDDNESTTSPDAVIGSVKGKRAILIDDIIDTGVRIANSAKTLERAGATEVFAVATHAVFSGNAVEELNNSPLKKVVFTDTVHIASLKDVLGDNLVVVSPDVGGVKLVRAYSKALGASLAIIDKRRVSGSETHVENLVGEVEGKNALVVDDMVSTGGSITDAARIVKAHGAKSVFLAATHGVLCGPAVERLDNAPVDGIFFSDREQYSKQGWKLQQLAAAEPVLAREFAKKKIPNLVIAASDMGGSKMARSFAERLGTSIAIVDKRRHHSHKVEALSLIGDVAGKNVLIPDDMISTAGTMVEAARFLQKHGAKDIYACCTHPVFSGSALEKIENSVIKEVMVTDNVPKDLKDKFPNIVLVKGRDVLAKAIEEIYAGG",
    "response#3": "MKVFAGNANPALAMKIADYIGIPMGNLVIQRFTDGETFVKICDNIRGKDVFVVQPLCYPPNEMIMELLIMVDAMKRASAERITAVLPYYGYARQDRKDQPRVPITAKLVANLLVAAGADEGLTIIGNKASGTGASPVLFEYLKTLDTDNLTLFSPDVGGMKMASAYAGMLQVPLGFIAKRRTDAETVEAVSLVGETDGRDILLIDDMTETAGTLCAAAELLKKNGANRVLASVSHGVLNNLGYERLSTGLLDRLITTDAPLLARYFVENKLDDDLVIVSPDHAGVSNARKLAELLHSPIAIIDNRDDNESTTSPDAVIGSVKGKRAILIDDIIDTGVRIANSAKTLERAGATEVFAVATHAVFSGNAVEELNNSPLKKVVFTDTIPVSEAANQLTDHAVVVSPDVGAVRRARDFAHRINLPLAIIEKRRSLDGHKTEMYNLIGDVAGMTCMLIDDEIDTAGTLTQAAYFLKEAGATDIIAFATHAVFSDPAAERLGDAPIDRLVVTNTIPLTQEMKLFPQLTILSVANLLGERIHRAMIGESVTALFN"
  },
  {
    "reference": "MLVTFLGILGLLASQVSSQLVGQLRPTEDPPEEELEYWCAYMESCQFCWDCQDGNCINKIDGSVIYKNEYVRPCSVSRSMDKCMYDLNKGIYHSMSCSDPKAWNPYKYFRKEWKKDEL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Plays a role in virus cell tropism, and may be required for efficient virus replication in macrophages.2. The designed protein localizes to the host cell endoplasmic reticulum lumen.3. The protein design must feature the Prevents secretion from ER motif.4. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "LHFDLHSGRTKCIAEDIKSNSMTVGKYNIDNPHEGQALPQTHKISVKVTSNSGNNYHHAEQVDSGQFAFSAVEAGDYMACFTAVDHKPEVSLSIDFEWKTGVQSKSWANVAKKSQVEVMEFEVKSLLDTVNSIHEEMYYLRDREEEMQDLNRSTNTKMAWLSVLSFFVCIGVAGMQFLHLKTFFP",
    "response#2": "VNMVQLPGGRFQMGTDSPDGRDGEGPVREVTVKPFAIDIFPVTNKDFREFVREKKYRTEAEVFGWSFVFEDLVSDELRNKATQRMQSLLWWLPVERAFWRQPAGPGSGIREKLEFPVVHVSWNDARAYCAWRGKRLPTEEEWEFAARGGLKGQVYPWGNKFQPNRTNLWQGKFPKGDKAEDGFHGVSPVNAFPPQNDYGLYDLVGNVWEWTASQYQAADQDMRVLRGASWIDTADGSANHRARVTTRMGNTPDSASDNLGFRCQ",
    "response#3": "LHFDLHSGRTKCIAEDIKSNSMTVGKYNIDNPHEGQALPQTHKISVKVTSNSGNNYHHAEQVDSGQFAFSAVEAGDYMACFTAVDHKPEVSLSIDFEWKTGVQSKSWANVAKKSQVEVMEFEVKSLLDTVNSIHEEMYYLRDREEEMQDLNRSTNTKMAWLSVLSFFVCIGVAGMQFLHLKTFFA"
  },
  {
    "reference": "MTAQTVPTTDDSGTPQDRPRLAAAVVLAAGEGTRMRSSTPKMLHTIGGRSLLGHVLTATRELDARRTAVVVRHQAERVAEHARAIAEGVLVVEQDELPGTGAAVRSALSVLDAAAHADRAAHGVPLGQEGQVLGDGLEGAVLVVAGDLPLLDAGTLGELVASHVADGNAVTVLTTVVADPTGYGRVVREDGTGDVLKIVEHRDASAEELEVAEINTSVYVFDAGVLRAGLARITQHNAQGELYLTDVVALAREDGGQVRAIVSEDPYAVEGVNDRVQLATLGRILNDRVLEDWMRAGVTIVDPATTWVDVDVDLAPDVTILPGTQLHGATVVSAGATIGPDTTLTDVEVGEGAVVSRTHATLSVIGAGANVGPFAYLRPGSVLGEKGKIGTFVETKNAHIGAGSKVPHLSYVGDATVGEGTNIGAATVFVNYDGVHKHHTTIGSHARTGADNLFVAPVTVGDGAYTGAGSVIRQDVPAGALGVSAGAQRNIDGWVERRRPGTAAAQAAAAARGEGVEPTGDDDSSDDAVVPATEPATLTGAMPALGAQALRELAKHADAADQPRPTSPHSEALDDTFTIRTARTEGTDR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have a high affinity for UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA.2. The protein have enhanced acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.3. A protein that can modulate the overall flux of metabolites through the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway, enhancing or suppressing its activity as needed.4. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.5. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "VIVLAAGEGTRMKSAKAKVLHSVAGRSLLGHVLHAVDHLKAKQVRIVVGSGRELVEEHISHIAPKASTVFQERRGGTGHAAQLALAGLSAKGTVLVLAGDTPMLTGESLAAFIDAHNEGKFAASVLTAEHPDPTGYGRIIRDDAGELGRVRVDEAGAVLGIVEHKDATEAQRRIREINSGIYAFDAAVLREGLGRLSTDNAQGELYLTDVLGIARADGGRVAAHVAGDPWLVEGVNDRVQLARLGAELNRRLLERWMRAGVTVVDPASTLIDATVTYERDAVVHPGTQLLGTTYLGEDCEVGPNSRLKDTLVHAGARVDNSVTDSAEIGAGASVGPYAYLRPGTRLGVKAKAGTYVEMKNATVGEGTKVPHLSYVGDATIGDHTNIGAASVFVNYDGVAKHHTTIGSHCRTGSDNMFVAPVTVGDGVYTAAGSVITKDVPSGSLAVARGQQRNIEGWVERKRPGTAAAEAADRALAEDDAAGREHAE",
    "response#2": "VIVLAAGEGTRMKSAKAKVLHSVAGRSLLGHVLHAVDHLKAKQVRIVVGSGRELVEEHISHIAPKASTVFQERRGGTGHAAQLALAGLSAKGTVLVLAGDTPMLTGESLAAFIDAHNEGKFAASVLTAEHPDPTGYGRIIRDDAGELGRILRDGDGRFLAIREHKDATEAERAVDLCNSGIMGFRAGLLRDLIGKIGNDNAKGEYYLTDVFGLAGVTIVDPTSTWIDVDVKLAADVTLLPGVQLLGATVVAEDAVIGPDSTLKDVEVGAGARVVRTHGELAVIGAGANVGPFSYLRPGTELGAGGKIGAFVETKNAQIGDGAKVPHLSYVGDATIGEGANIGAGTIFANYDGVEKHRTVIGAHAKTASNNTFVAPVTVGDGASTGAGSVIRRDVPPGSLAVTSGPDLAEAEAAFQARRRQEMMESGVTLIAPDTVFFSHDTRIAADVVVEPNVVFGPGVNVASNVRIKAFSHLEGASLGEGAEAGPFARLRPGAELGANAKVGNFVEVKKARLGEGAKASHLTYLGDTDVGAHANIGAGTITCNYDGFFKHRTVIGEGAFIGSNTSLVAPVTIGAGAMTGSGSVITEDVPDNALALSRARQVSKEGWADRFRAAQAARKKA",
    "response#3": "VVILAAGQGTRMRSDLPKVLQPLAGRPLLEHVIDVAEAVQSKQICVVYGHGGDRVKAQFANRNIDWVLQAEQLGTGHAVMQAMPFVQPDQTVLILCGDVPLVRAETVVVLAAAADSDLGVLTALVADPTGYGRIVRDADGTVAAIVEHKDASDAQRGIREINTGILAAPSATLRGWLARLDNRNAQGEYYLTDVIALAVADGVPVVTAQPAGVWETLGVNSKSQLAELERGHQNRTADALMAAGVSLINPANTYIGVDVKIGSDTVIQPGVMLEGNTVIGEDCHIGPNSRITNSTIGDRTKVDSSVVDSSVVGSDVAVGPFAHIRPATNVGNRAKVGNFVELKKTTLGEGSKASHLTYLGDAEIGTDVNIGCGTITVNYDGVNKFKTIIGDRSFIGCNANLVAPVTINSDTYVAAGTTITKEVPPFALAIGRSRQVSIAGWKRPKKG"
  },
  {
    "reference": "MDVSNTMLLVAVVAAYWLWFQRISRWLKGPRVWPVLGSLPGLIEQRDRMHDWITENLRACGGTYQTCICAVPFLAKKQGLVTVTCDPKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILETAQNNYEPVDLQDLILRLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFILPEFLWRLKKWLGLGLEVSLSRSLGEIDGYLDAVINTRKQELLSQRESGVQRHDDLLSRFMKKKDQSYSETFLRHVALNFILAGRDTSSVALSWFFWLITTHPTVEDKIVREICSVLIETRGTDVSSWTAEPLEFDEVDRLVYLKAALSETLRLYPSVPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAAGRMKSTWGEDCLEFKPERWISPDDGKFVNHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRHRLTVAPGHKVEQKMSLTLFMKNGLLVNVHKRDLEVMMKSLVPKERNDVVVLNGKCNGGIGEGVAVNAAVAVAV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The heme binding site should be located in a region of the protein that is accessible to the ligand.2. The presence of heme is necessary for the protein to function in the desired environment.3. The designed protein must be optimized for heme binding.4. The protein must exhibit the following characteristics: Involved in the biosynthesis of hydroxylated fatty acids required for cutin biosynthesis, cuticle development and repression of bacterial type III gene expression.; Plays a major role in the biosynthesis of extracellular lipids; Acts on saturated and unsaturated fatty acids with chain lengths from C12 to C18. The designed protein sequence is ",
    "response#1": "EAGE",
    "response#2": "MAPKRSGGKGGKK",
    "response#3": "GHISLPR"
  },
  {
    "reference": "MADWLYQYPPLLAGTLIKRYKRFLADIELDTGEVVTAHCPNTGPMTGMCAPHSRVQISQSNNPKRKLAYTWEMIEVCDTEPTWVGVNTQIPNLVVKQLLQNRLLPTLGEYDQVKSEVRYGREKSRIDFLLTGTAQPAYIEVKSTTWAIEKQALFPDTVTIRGQKHIRELISVLPAAQAYLLFFINRADCTTFAPGDQADPEYGKLLREAVATGVGLLPCRFEITPEGIRYLGLATVEESSLTR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit DNA binding. The designed protein sequence is ",
    "response#1": "LPNRLVREAIEDGTVTELQGYGSIRPEVRYGEERSRIDLLLEGEPGRCWVEVKNVTLVEGERALFPDAVTERGQKHLRELMEMVRRGDRGVIFYVVQRGDGEAVSPADAIDPKYGQLLRLAVANGVEALAYRALVTPQ",
    "response#2": "LPNRLVREAIEDGTVTELQGYGSIRPEVRYGEERSRIDLLLEGEPGRCWVEVKNVTLVEGERALFPDAVTERGQKHLRELMEMVRRGDRGVIFYVVQRGDGEAVSPADAIDPKYGQLLRLAVANGVEALAYRALVTPR",
    "response#3": "LPNRLVREAIEDGTVTELQGYGSIRPEVRYGEERSRIDLLLEGEPGRCWVEVKNVTLVEGERALFPDAVTERGQKHLRELMEMVRRGDRGVIFYVVQRGDGEAVSPADAIDPKYGQLLRLAVANGVEALAYRALVTPR"
  },
  {
    "reference": "MENVLRVVILGFIQGIAEFLPISSSGHLLLLKKFMHIDLPIVFDIYLHLATVLVVMIYYCRRILELVAVLVKFVLGKTTECDFAKLRLILLILIITIITAFIGIFIEMFKGLFTLNLVLINFIVTSILLFLLESRIVIFDLKRNILLAGCLIGTMQGIGAMPGISRSGITIFASVLLGFSRSESFEISFLSLIPIVFGSLLLKYKELFESDMLFSIFEINLGAIIAFLVGLFSISLFVKMLQDSKLYYFSVYLIILVSLVYFLF",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP)2. The designed protein must be able to regulate cell wall organization. The designed protein sequence is ",
    "response#1": "GTTLIDSDSTYIGADVVIESDTIIYPGNVLQGETVIKTGCILYPNSRIQDSVIGKNVTVQSSVILESTVGEDTTVGPFAYIRPESKIGNKARIGDFVEIKKSTIGDNTKVSHLTYIGDAEVGEGCNFGCGTVVVNYDGKAKHKTVIGNKSFIGCNTNLVSPVKVEDNTYIAAGSTITNTVPEGSLAIARAKL",
    "response#2": "GTTLIDSDSTYIGADVVIESDTIIYPGNVLQGETVIKTGCILYPNSRIQDSVIGKNVTVQSSVILESTVGEDTTVGPFAYIRPESKIGNKARIGDFVEIKKSTIGDNTKVSHLTYIGDAEVGEGCNFGCGTVVVNYDGKAKHKTVIGNKSFIGCNTNLVSPVKVEDNTYIAAGSTITNTVPEGSLAIARAKM",
    "response#3": "GTTLIDSDSTYIGADVVIESDTIIYPGNVLQGETVIKTGCILYPNSRIQDSVIGKNVTVQSSVILESTVGEDTTVGPFAYIRPESKIGNKARIGDFVEIKKSTIGDNTKVSHLTYIGDAEVGEGCNFGCGTVVVNYDGKAKHKTVIGNKSFIGCNTNLVSPVKVEDNTYIAAGSTITNTVPEGSLAIARAKV"
  },
  {
    "reference": "MRIGVLTGGGDCPGLNPAIRGLVMRSLDFGHECLGYQNGWKGLVEGVSRPLGLADVEDIIGLGGTILGSSRTNPFKKGREEDLEKCLATIKRHELDAVVAIGGEDTLGVAHKLFELGISTVGVPKTMDNDLNATDFTFGFDSAVAVATESIDRLRDTARSHSRVMVVEVMGRHAGWVALYAGIAGGADWILLPEVPLDLDAMCAHLLHCRKRGKNYGIVVASEGVAVPQSSDAEMAVDAFGHVILRERGVGEYVAREVEKRTGIETRFAVLGHIVRGGPPTVFDRVLASRLGIKAAELVNAGEFGKMASIRGTEIVAVPLKDALEKLKLVPPWQYQEITTLFSK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis; Consistently, PPi-PFK can replace the enzymes of both the forward (ATP- PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.; Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis.2. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in beta-D-fructose 6-phosphate + diphosphate = beta-D-fructose   1,6-bisphosphate + H(+) + phosphate are properly positioned for optimal catalysis.3. The ideal protein would be one that plays a critical role in fructose 6-phosphate metabolic process through 6-phosphofructokinase activity, ATP binding, diphosphate-fructose-6-phosphate 1-phosphotransferase activity, metal ion binding within cytoplasm.4. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "MKKKIGVLTGGGDCPGLNAVIRSVVRKGTQEYGFEFVGLRDGWLGALRDKVVPLDIAAVRGILPRGGTILGSSRTNPFKHEDGVARIKDTLTAHGVDALVVIGGEDTLGAATELSRQGVPLVGVPKTIDNDVAGTDYTFGFDTAVGIATEAIDRLHTTAESHMRTLVVEVMGRHSGWIALHAGVAGGANVILVPERPFDIDQVCGWVKNRFRTNYAPIVVAAEGAVPKEGHVIVQDRALDEYGHVHLSGIGEWLAQEISRRTGTDARTTVLGHIQRGGTPSAFDRWLATRFGLHAADAASRGEWGTMVALRGTSIVRVPLSEATTELKTVPLERYEEAEVFFG",
    "response#2": "NVGVILSGGQAPGGHNVIAGIFDGIKRLHKESKLYGFILGPAGLIDHQYKELTSEIIDEYRNTGGFDIIGSGRTKLETKEQFDKGLIILKKLDIKALVIIGGDDSNTNACVLAEYYKSINAGVQVLGCPKTIDGDLKNEQIETSFGFDTACKVYSELIGNIQRDCNSSRKYWHFIKVMGRSASHIALECALQTHPNVCIISEEVEEKDLSLDDIIEYIAGVIAKRAENGLNFGTVIIPEGLIEFIGWLDGEEIRTGERVGVLTGGGDCPGLNAVIRAVVRKGIEVHGWDFVGFRNGWNGPLTGDSRPLGLNDVEDILTRGGTILRSSRTNPYKVEGGVDKIKQVLADQGVDALIAIGGEDTLGVAKRLTDDGIGVVGVPKTIDNDLGATDYTFGFDTAVSIATEAIDRLHTTAESHHRALVVEVMGRHAGWIALHSGLAGGASVILVPERHFNVDQVVSWVERRFEKEFAPIIVVAEGALPEGGEEKLLTGEKDAFGHVRLGGIGTWLADEIAHRTGKESRAVVLGHVQRGGTPTAYDRVLATRFGLNAVDALMNGEYDTMPVVKGALVTREMKNVSLEEYKESFKTVDWFFEMYDYIKEGKH",
    "response#3": "KIGVLTGGGDCPGLNAVIRSVVRKGTQEYGFEFVGLRDGWLGALRDKVVPLDIAAVRGILPRGGTILGSSRTNPFKHEDGVARIKDTLTAHGVDALVVIGGEDTLGAATELSRQGVPLVGVPKTIDNDVAGTDYTFGFDTAVGIATEAIDRLHTTAESHMRTLVVEVMGRHSGWIALHAGVAGGANVILVPERPFDIDQVCGWVKNRFRTNYAPIVVAAEGAVPKEGHVIVQDRALDEYGHVHLSGIGEWLAQEISRRTGTDARTTVLGHIQRGGTPSAFDRWLATRFGLHAADLVRGNGTAMVVALDGERAVVLGHLQRGGTPSPRDRIGVLTGGGDAPGLNAAIRAVVKRCEKYGYEVVGIRYGWAGMLKKDVIKLTYKDVKDTINRGGTILKTSRTNPLKQPNGIEMVSKNFKELGLDALIAIGGDDTLSVAKALSEAGLKVVGIPKTIDNDVSETDATIGFDTAVNTAMRIIENLRDTAESHERVIVVEIMGRYAGWIALNSGLAAGADLILIPEEPFKIKDVTEFVKRKVAMGERSILIVVAEGALIADYKGPITKDTSVDQFGHVRLGGIGEFLAKKIREELGVETRAITPAHIVRGGSPTAFDRLMATRYGIAAVDLVRSGSFGRMVALRGSDIVEVPLAEGVAATKTVPAELYEVAHVFFG"
  },
  {
    "reference": "MNYANFGLELFKSIADAAYEGKRVELAGKTLALKNRALDTERDYNYARLAFEKQRFETNADLRVNGDLYRMQALRAAGYRLNPFSNGHQIYADEAAAANLHSYYGFYKTD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein localizes to the host cell cytoplasm, virion component. The designed protein sequence is ",
    "response#1": "KEEMKNCSFNVTTLLRDKKEKKEALFYRLDIVPLNGKNSSKNSSEYYRLINCNTTAMTQACPKVTFDPIPIHYCTPAGYAILKCNEKNFTGIGKCSNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIISSXNLTDNGKIIIVRLNQSVEIVCTRPNNNTRKGIRIGPGQTFYATGDIIGDIRLAHCNISKDDWNKTLQKVSKKLAEHFPNKRINFTSSSGGDLEIVTHSFNCRGEFFYCNTSGLFNFTYMPNSTDMPLNDTTNNSRSVITLPCRIKQVINMWQEVGRAMYAPPIEGNITCKSNITGLLLFRDGGKGENTTEIFRPGGGDMKDNWRSELYYYKVVEIKPLYCIAPTTAERRKVVEREKR",
    "response#2": "MGAASITLTVQARQLLSGIVQQQSNLLKAIEAQQHLLQLTVWGIKQLQTRVLAIERYLQDQQLLGIWGCSGKLICTTAVPWNSSWSNKSQEEIWENMTWMQWDKEISNYTYTIYRLLEDSQSQQERNEKDLLALDSWNTLWSWFNITNWLWYIRIFIMIVGGLIGLRIVFAVLSVVNRVRQGYSPLSFQTL",
    "response#3": "MGAASITLTVQARQLLSGIVQQQSNLLKAIEAQQHLLQLTVWGIKQLQTRVLAIERYLQDQQLLGIWGCSGKLICTTAVPWNSSWSNKSQEEIWENMTWMQWDKEISNYTYTIYRLLEDSQSQQERNEKDLLALDSWNTLWSWFNITNWLWYIRIFIMIVGGLIGLRIVFAVLSVVNRVRQGYSPLSFQTI"
  },
  {
    "reference": "KRVQNEGTGKSSWWMINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKASLQASQEATEDSPSQLTKWPGSPTSRSSDELDTWTDFRSRTNSNASTISGRLSPILATTELDEVQDDDPPLSPMLYSNSSSMSPSVSKPCTVELPRLTDMAGTMNLNDGLTDNLMDDLLDNITLPPSQQSPSSGLMQRSSSFPYTAKGSGLGSPSGSLNNAVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYSNQSLQDLLTSDSLSHSDVMMTQSDPLMSQASTAVSAQNSRRSVMLRNDPMMSFAAQSNQGSLVNQNLLHQQHQTQSPLGGNRALSNAVNSMGLSDANGLGSAKHQQQSPVSHSMQTLSDSLSGSSLYSTSVNLPVMGHEKFPSDLDLDIFNGSLECDMESIIRSELMDADGLDFNFDSLISAQNVVSLNVGNFTGAKQASSQSWVPG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have DNA-binding transcription activator activity, RNA polymerase II-specific, mitochondrial transcription factor activity, DNA-binding transcription repressor activity, RNA polymerase II-specific within cytosol, nucleus to regulate positive regulation of muscle atrophy, response to starvation.2. The protein contains novel FOXO protein transactivation domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "IPPPIFSRLDTPVDYFYRPETQHREGYHNPTISGENLIGLSRARRPHNAIFVNFEDTEVPEQPLEAAVQTWKKACTNPIDQKVEEELRKLFDIRPVWSRNAVKSNVSVHPDKLKILLPYMAYYMITGPWRSLWIRFGYDPRKHPDAKIYQVLDFL",
    "response#2": "IPPPIFSRLDTPVDYFYRPETQHREGYHNPTISGENLIGLSRARRPHNAIFVNFEDTEVPEQPLEAAVQTWKKACTNPIDQKVEEELRKLFDIRPVWSRNAVKSNVSVHPDKLKILLPYMAYYMITGPWRSLWIRFGYDPRKHPDAKIYQVLDFL",
    "response#3": "KSSILKPDEVLNRITKRMMSSCSVEEEALVAISDAVESHLRELITLMAGVAEHRVESLRIPENYVAIDDVKRQLRFLEDLDRQEEELRESREKESLIG"
  },
  {
    "reference": "MFKFNTIEEGLEDIRNGNFVIVVDDKNRENEGDLIVAAEKITDSKINFMIKYARGLVCMPIIEERLKELNIEQMVKENTDNHETAFTVSIDYKDTITGISAGERATTIKKVLDPNVTGEDFRRPGHVFPLKGQEGGVLKRAGHTEAAIDLARLAGLYPAGVICEIINEDGTMARIPQLMDFAKKHNIKIITIADLIAYRIKNEILVSKVAETRLPTIYGDFSIMGYVNKINGEHHLALVKGDINCDEPILVRVHSECLTGDVFASLRCDCGGQLHESMKRINEEGKGILLYMRQEGRGIGLINKLKAYELQEKGMDTVEANLALGYPEDLRDYGIGAQILSDIGAKKIKLMTNNPKKIYGLSNYGLEVVERVPIEIESNDEDYFYLKTKKEKMGHILNIK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The Zn(2+), GTP, D-ribulose 5-phosphate, Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.2. The presence of Zn(2+) is necessary for the protein to function in the desired environment.3. A key design requirement for the protein is the optimization of the Nucleophile; for GTP cyclohydrolase activity, Proton acceptor; for GTP cyclohydrolase activity to achieve high catalytic efficiency for GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.4. A protein that is capable of catalyzing reactions within the riboflavin biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4, Cofactor biosynthesis pathway with high efficiency and specificity.5. The designed protein must have at least one GTP cyclohydrolase II domain.6. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..7. A protein with magnesium ion binding, GTP cyclohydrolase II activity, zinc ion binding functions is required to enable riboflavin biosynthetic process. The designed protein sequence is ",
    "response#1": "RLAETVLPTAIAGEFKAIVYESEVDPNHHLALVKGEIDSEQPILVRVHSQCLTGDVFGSLRCDCGSQLHTAMKMIEKEGKGVLVYMRQEGRGIGFANKMKAYALQDAGHDTVEANLALGFKPDLRNYGIGAQILRDLGVRKMRLMTNNPKKIVGLEGYGLEVVERIVSVEDVFPDGRSREVEARVPTEHAGEFKAVVYSNSVDHLDHLALVKGDISKAERVLVRVHSECLTGDVFGSARCDCGLQLNAAMKMIDQEGCGIVLYMRQEGRGIGLVNKLKAYCLQDDEGVDTVEANHRLGFKSDLRDYGIGAQILRDLGVKKMAILTNNPKKIVGLEGYGIEVVERLMDAGEYFRG",
    "response#2": "RLAETVLPTAIAGEFKAIVYESEVDPNHHLALVKGEIDSEQPILVRVHSQCLTGDVFGSLRCDCGSQLHTAMKMIEKEGKGVLVYMRQEGRGIGFANKMKAYALQDAGHDTVEANLALGFKPDLRNYGIGAQILRDLGVRKMRLMTNNPKKIVGLEGYGLEVVERIIGMRGGELWCVDERVAEAALPTAYGEFRAITYRTLIDAKEHQAFVMGNVDTDDPVLARVHDQCVTGDVFGSLRCDCGEQLEEAIRRVASEHRGVIVYMDQEGRGIGLHNKIRAYRLQDSGMDTVEANEALGLPADGREYGIGMQILVDLGVRKLRLMTNNPTKRSGLEGFGLEIVERVERVAEAALPTAYGEFRAITYRTLIDAKEHQAFVMGNVDTDDPVLARVHDQCVTGDVFGSLRCDCGEQLEEAIRRVASEHRGVIVYMDQEGRGIGLHNKIRAYRLQDSGMDTVEANEALGLPADGREYGIGMQILVDLGVRKLRLMTNNPTKRSGLEGFGLEIVERVERVAEAALPTAYGEFRAITYRTLIDAKEHQAFVMGNVDTDDPVLARVHDQCVTGDVFGSLRCDCGEQLEEAIRRVASEHRGVIVYMDQEGRGIGLHNKIRAYRLQDSGMDTVEANEALGLPADGREYGIGMQILVDLGVRKLRLMTNNPTKRSGLEGFGLEIVERVI",
    "response#3": "RLAETVLPTAIAGEFKAIVYESEVDPNHHLALVKGEIDSEQPILVRVHSQCLTGDVFGSLRCDCGSQLHTAMKMIEKEGKGVLVYMRQEGRGIGFANKMKAYALQDAGHDTVEANLALGFKPDLRNYGIGAQILRDLGVRKMRLMTNNPKKIVGLEGYGLEVVERIASARMPTKYGDFQIYGYVNDITGEHHVALVKGDLGDGENVLCRVHSECLTGDTFGSRRCDCGEQLQRAMQQVEKEGRGIILYMRQEGRGIGLINKLKAYELQEQGLDTVEANIRLGFDADLREYWVGAQILADLGARSLRLLTNNPDKIYGLEGFGLTITERVPYEEVPDVEVDMPTKYGHFHLIPFLQKSNGLEHIALIKGKWDEDEEILVRVHSSCMTGDIFGSLRCDCGDQIHKAMELIEKEGRGVIVYLMQEGRGIGLMNKMKAYKLQEEGLDTVEANIELGFAADERDYGIGAQILRQLNVRKMRLLTNNPVKRIGLEGYNLQIVGNREVEVKMPTEFGVFKAVAYTNDVDNKEHVALVKGEIDSSQPTLVRVHSECLTGDVFHSHRCDCGPQLEAALRQIDSEGSGVLLYMRQEGRGIGLINKLKAYKLQEEGLDTVEANQKLGFAPDLRDYGIGAQILKDLGVSQIKLLTNNPKKIRGLEGYGLEVVERVPYEEV"
  },
  {
    "reference": "MIHDPAQLEKERPDRLRVLWLSTLAFTLMFAAWLMFGVLGVPIRKEFGLSDVQLSWISALAILNGSLWRLLAGILADRYGGRLVFTLMLFFTAIPAYLVSRAGSYEELLLYAFLVGFAGNSFSVGIAWNSAWFPKDQQGFALGVFGAGNVGASVTKFIGPALIASVPASGYLGGLIPGGWRFIPFLYAVLLVLMGFVLWFGTPRKDKRPGQGRPFLDMLKPLRYVRVWRFSLYYVVVFGAYVALSAWLPKYYVDVFGLPLHEAALLTALFIFPASLLRPVGGYLSDRFGARRIMYWTFGIILLASGVLMMPEGHIVLYTKQGAKEVMQFTMGVELFTLLVFLIGVGMGIGKAAVYKHIPTYFPQDVGAVGGLVGMLGALGGFFLPPLFAYAQAWTGLPQMTFFVLFLLAAISFLWMHLTVLKLLQQEAQHLKNDFELKGDRPC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must be able to antiporter activity.2. The protein must exhibit the following characteristics: Probable nitrate/nitrite antiporter that may be involved in nitrate import and nitrite export during anaerobic growth.. The designed protein sequence is ",
    "response#1": "ILHQTWFAFFLTFVCWFNFAPFATTIGKELHLAPEQIKTLGICNLALTIPARLIIGMLLDRFGPRITYSILLMFAVVPCLATALAQDFNQLVISRLLMGIVGSGFVVGIRMVAEWFQPKEMGIAQGIYGGWGNFGAFGAEFALPILAISTSFFSGGASNWRLAIALVGIITAIYGVIYYNTVQDTPRGKVYKKPKKNGSLEVTSIKSFWAMMISNFGLIFALGLLAWRLEQKKIHFLTLSQMYLTWLVLAGLFAYQSYKAWQVNRELLTGKKTYPVSERFQFGQVALLEFTYITNFGSELAAVSMLPAFFEKTFGLEHVVAGMIAATYPFLNLVSRPSGGLISDKFGSRKWTMTIISVGIGVSYLMAHFINSNWPIPVAIAVTMFAAYFAQAGCGATYSIVPMIKKEATGQIAGNVGAYGNFGGVVYLTIFSLTDAPTLFSTMGIAALICAFMCAFFLKEPSR",
    "response#2": "QLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLGQAQTPGMLMASGFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQTTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGAFVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDFVIMIIGAVILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFSNEAGSANGIVSMMGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKER",
    "response#3": "ILHMTWFAFFLSFVVWFNFPPFATTIAQDFGLDKAQLGTIGLCNVALTVPARIIIGMLLDKYGPRLTYSLLLIYAAVPCLIFATAQSFNQLVLGRLLMGIVGAGFVIGIRMVAEWFPPKDVGTAEGIYGGWGNFGSAFSAFTMVIFGIILAFLPGAFNFGQPESFKILFFPEFNTAILNWRAAIAGTGIIAALYGMLYYFSVSDTPPGKTYHRPKSARGMEVTTKKDFWFLLAMNLPLTLILMVLAWRLQKVNFLNGTGFAIAILALVGLYLFQTYNCWTVNKDLMTGKKRYAPEDRYEFSQVAILELTYIVNFGSELAVVTMLPAFFEGTFSLDKATAGIIASSYAFMNLMSRPGGGLISDKMGSRKWTMVVLTVGMGVGYLLMSSVAGTWPLAIAVLLTMACSFFVQAAEGSTFAIVPLVKRRITGQIAGNVGAYGNVGAVAYLTVLLLLTEASAGANGGEPVMATVNAGFFQVLGITGLIVAFLCAFFLKEPKRL"
  },
  {
    "reference": "GLGSLLGKAFKIGLKTVGKMMGGAPREQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in hemolysis in another organism, potentially with improved properties such as stability, specificity or selectivity.2. The protein must exhibit the following characteristics: Peptide CPF-B1: Has antimicrobial activity against Gram- negative bacteria E.coli ATCC 25922 (MIC=5 uM) and multidrug-resistant A.baumannii (MIC=4-8 uM), against Gram-positive bacteria S.aureus ATCC 25923 (MIC=5 uM) and methicillin-resistant S.aureus and against fungus C.albicans ATCC 90028 (MIC=25 uM). The designed protein sequence is ",
    "response#1": "GIFSLIKGAAKLITKTVAKEAGKTGLELMACKVTNQC",
    "response#2": "PFLLGMVPKLVCLITKKC",
    "response#3": "RKGTCHFGGCPAHLVKVGSCFGFRACCGR"
  },
  {
    "reference": "MSISKDDILNAVAEMSVMDVVELITMMEEKFGVSAAAAVAVAAGPAEAAEEKTEFDVILKGIGGNKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKDDAEALKKALEEAGAEVEVK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Is thus essential for accurate translation.2. The designed protein should have a specific function within the ribonucleoprotein complex. The designed protein sequence is ",
    "response#1": "MNHVRKGDLVQVITGKDAGKKGKIIEVIPN",
    "response#2": "MTPQP",
    "response#3": "MPN"
  },
  {
    "reference": "MVGIRQYGVFTWVFRTFQLAIDTIVLALASALVNQQTSGGSPGKINFSVAVGSFAILTFFLTAVGRFLPTILGNPWLIAFYDFVNWVFALTGGCCIAVAIRVHACDNQKYLDRNHYTQGSMRRCQELKALCFFLWFMFGLYVASFIVQIFIAKNDTPNYTFRGRGRGKGSGPAVAPRPVMSAV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Cytoplasmic, Extracellular topological domain of the protein should be designed to be flexible or rigid.2. For general function, the protein need meet that Involved in membrane organization and might act as a sensor of sphingolipids that regulates plasma membrane function; Involved in a novel pathway of export of proteins that lack a cleavable signal sequence.3. The protein should be optimized for stability and solubility when expressed in plasma membrane, eisosome, Golgi membrane, growing cell tip where it will be involved in eisosome assembly, protein transport, plasma membrane organization, protein localization to plasma membrane.4. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "DVEKAEWLNKIVAQVWPFLGQYMEKLLAETVAPAVRGSNPHLQTFTFTRVELGEKPLRIIGVKVHPGQRKEQILLDLNISYVGDVQIDVEVKKYFCKAGVKGMQLHGVLRVILEPLIGDLPFVGAVSMFFIRRPTLDINWTGMTNLLDIPGLSSLSDTMIMDSIAAFLVLPNRLLVPLK",
    "response#2": "AGPRGALLAWCRRQCEGYRGVEIRDLSSSFRDGLAFCAILHRHRPDLLDFDSLSKDNVFENNRLAFEVAEKELGIPALLDPNDMVSMSVPDCLSIMTYVSQYYNHFN",
    "response#3": "DVERVEWANKIIIQIWPYLSMIMENKIREKLEPKIREKSIHLRTFTFTKLYFGQKCPKVNGVKVHTDKRNRRKVTLDLQICYIGDCEISVELQKIRGGVSGVQLQGTLRVILEPLLVDKPFIGAVTVFFLQKPHLQINWTGLTNLLDMPGINELSDSLLEDLIAAHLVLPNRVTVPPKGSK"
  },
  {
    "reference": "MVGRMLSSPEPTLSTMAMSAAHGEDSPYFAGWRAYDEDPYDPITNPQGVIQMGLAENQVSFDLLEEYMREHPEASDCGAGVRENALFQDYHGLKSFRKAMASFMETIRGGKARFDPDRVVLTAGATAANELLTFILADPGDALLVPTPYYPGFDRDLRWRTGVNIVPVSCDSAAGFQVTAGALRAAYDEAVAAGTRVRGVLITNPSNPLGTTAARGVLEGILDFVARHDMHLISDEIYSGSVFAAPDLVSVAELVDERRRARGGAADAEDIARRVHVVYSLSKDLGLPGFRVGVVYSYNDAVVAAARRMSSFTLVSSQTQRTLAAMLSDAAFAAAYVRSNRDRLRERHARAVAGLRRAGVACLRGANAGLFVWVDMRRLLGDGEATVAGELRLWRRVVAEAKLNISPGSSCHCREPGWFRVCFANMSLETLDVALHRLGCFIKKWEQEQHEN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein should be able to manipulate the activity of enzymes in the ethylene biosynthesis via S-adenosyl-L- methionine pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "QMGLAENQLTSDLVEDWILNNPEASICTPEGINDFRAIANFQDYHGLAEFRNAVAKFMARTRGNRITFDPDRIVMSGGATGAHEVTAFCLADPGEAFLVPIPYYPGFDRDLRWRTGVKLVPVMCDSSNNFVLTKEALEDAYEKAREDNIRVKGLLITNPSNPLGTIMDRKTLRTVVSFINEKRIHLVCDEIYAATVFSQPGFISIAEILEDETDIECDRNLVHIVYSLSKDMGFPGFRVGIIYSYNDAVVNCARKMSSFGLVSTQTQYLLASMLNDDEFVERFLAESAKRLAQRFRVFTGGLAKVGIKCLQSNAGLFVWMDLRQLLKKPTFDSETELWKVIIHEVKINVSPGYSFHCTEPGWFRVCFH",
    "response#2": "QMGLAENQLTSDLVEDWILNNPEASICTPEGINDFRAIANFQDYHGLAEFRNAVAKFMARTRGNRITFDPDRIVMSGGATGAHEVTAFCLADPGEAFLVPIPYYPGFDRDLRWRTGVKLVPVMCDSSNNFVLTKEALEDAYEKAREDNIRVKGLLITNPSNPLGTIMDRKTLRTVVSFINEKRIHLVCDEIYAATVFSQPGFISIAEILEDETDIECDRNLVHIVYSLSKDMGFPGFRVGIIYSYNDAVVNCARKMSSFGLVSTQTQYLLASMLNDDEFVERFLAESAKRLAQRFRVFTGGLAKVGIKCLQSNAGLFVWMDLRQLLKKPTFDSETELWKVIIHEVKINVSPGYSFHCTEPGWFRVCFH",
    "response#3": "QMGLAENQLTSDLVEDWILNNPEASICTPEGINDFRAIANFQDYHGLAEFRNAVAKFMARTRGNRITFDPDRIVMSGGATGAHEVTAFCLADPGEAFLVPIPYYPGFDRDLRWRTGVKLVPVMCDSSNNFVLTKEALEDAYEKAREDNIRVKGLLITNPSNPLGTIMDRKTLRTVVSFINEKRIHLVCDEIYAATVFSQPGFISIAEILEDETDIECDRNLVHIVYSLSKDMGFPGFRVGIIYSYNDAVVNCARKMSSFGLVSTQTQYLLASMLNDDEFVERFLAESAKRLAQRFRVFTGGLAKVGIKCLQSNAGLFVWMDLRQLLKKPTFDSETELWKVIIHEVKINVSPGYSFHCTEPGWFRVCFH"
  },
  {
    "reference": "MGGKWSKSSIVGWPKVRERIKQTPPAAEGVGAVSQDLDKHGAVTSSNINNPDCVWLEAQEEEEVGFPVKPQVPLRPMNYKSAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYHTQGFFPDWHNYTPGPGVRFPLCFGWCFKLVPVDPSEVEEDNKGENNSLLHPMSQHGIEDEHREVLMWKFDSGLAREHIARRLHPEXYKDC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK12. The designed protein should contain at least one Diacidic; necessary for CD4 internalization, PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif to achieve its intended function.3. A protein that contributes to suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II by functioning in extracellular region, host cell Golgi membrane, membrane, virion component, host cell plasma membrane with GTP binding. The designed protein sequence is ",
    "response#1": "MGGKWSKRSVPGWPAVRERLRRAPPAAEGVGAASQDLARHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQQRQDILDLWVYNTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVAPEEVEKANEGENNSLLHPICQHGMDDEEREVLVWKFDSRLAFHHVAREKHPEFYKDC",
    "response#2": "MGGKWSKSKGVGWSAVRERMRRTEPAAEGVGAASRDLEKYGAITSSNTAANNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPEEVEKANEGENNCLLHPMNQHGMDDPEKEVLQWKFDSRLAFHHMARELHPEYYKN",
    "response#3": "MGGKWSKSSIVGWPAIRERIRRTEPAADGVGAVSRDLEKHGAITSSNTDHNNAACAWLEAQEEEEVGFPVRPQVPLRPMTYKAAIDFGHFLKEKGGLDGLIHSQRRQDILDLWVYNTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPREVEEANEGENNCLLHPMSLHGMEDEHGEVLKWKFDSSLARRHMARELHPEFYKDC"
  },
  {
    "reference": "MLPNEELVPVKRITPQSSWSWISIDATGKKTVLDVDKYVIMHRVQIHARDLRILDPNLFYPSAILGRERAIVLNLEHIKAIITAKEVLIQDSSDENLIPTLEEFQTRLSVGNKAHGGQLDGDVVEEDESAFEFRALEVALEAICSFLAARTIELEKSAYPALDELTLKLTSRNLLRVCKLKSSMTRLTAQVQKIKDELEQLLEDDEDMAELYLSRKLAGASSPAIDSGEHINWYPTSPTIGAKISRAKSHLVRSATVRGDDKNDVEEVEMLLEAHFYANRQNFEQINRATRVCG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. Ensure the presence of a signal peptide within the protein.2. The designed protein must possess magnesium ion transmembrane transporter activity to support magnesium ion transport under certain conditions. The designed protein sequence is ",
    "response#1": "TPVINGIGGNLVAIQASRISTYLHLHSIPGELPEEAKGCYYPCRTYYGTGVNNKSAQVLLLLVIPGHLIFLYTIHLMKSGHTSLTPIFIAVYLFAALLQVFTLLWIADWMVHHFWKKGKDPDSFSIPYLTALGDLLGTALLF",
    "response#2": "TPVINGIGGNLVAIQASRISTYLHLHSIPGELPEEAKGCYYPCRTYYGTGVNNKSAQVLLLLVIPGHLIFLYTIHLMKSGHTSLTPIFIAVYLFAALLQVFTLLWIADWMVHHFWKKGKDPDSFSIPYLTALGDLLGTALLF",
    "response#3": "TPVINGIGGNLVAIQASRISTYLHLHSIPGELPEEAKGCYYPCRTYYGTGVNNKSAQVLLLLVIPGHLIFLYTIHLMKSGHTSLTPIFIAVYLFAALLQVFTLLWIADWMVHHFWKKGKDPDSFSIPYLTALGDLLGTALLF"
  },
  {
    "reference": "MERLAGSGTAVMDGYDLITTKEMRVLELNAEYFGVSRLQLMENAGRNVAVEVASRFKPQEARITVFCGLGGNGGDGFVAARHLLCLGFKVEVILAGRPSNITSEEAKKNWQALESLGDSITVKEVYDSSLVPEVEAEVVIDALLGIGVKGRLRPPILQIVKAINEAEAFRVAVDVPTGIDSDTGEVLGEAVRADLTVTFHKAKPGLLKAKEYVGDLVVRHIGLPEEIERYAGPGDVYAVVKPRPPESHKGDFGRLLVIGGSAVYFGAPALVASAALRTGVDLAYIAAPEKTAQAISTMSPNLITFKLEGDHLNRLNIPVIRRCLEKATAVVVGPGLGLHKETEEALKEVLRIVEERGIPVLLDADGLKAFANFKRRLRVPLVLTPHAGEYKILTGDTLPGKLKDRIEMVRRAAQELNAVILLKSNVDVISDGVRVKLNFTGNPGMTVGGTGDVLSGIVGAFLAQGADPFNAAVAGAFINGAAGDFVKAEKGYHMLPTDLLEWIPRVIDDPMSHVKVRRSVPRGL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The presence of Mg(2+) is necessary for the protein to function in the desired environment.2. The K(+) binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein must possess ADP-dependent NAD(P)H-hydrate dehydratase activity to support nicotinamide nucleotide metabolic process under certain conditions.4. The protein should be designed to catalyze the following reaction (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "VGVVAGSARYPGAAVLAVSGALRGGAGAVRYVGPGGEAVLARFPETLVHEGPPSGAGRVQAWAVGPGLGDGSWAAAAVADVLAADVPVLVDADGLRLLAERTVRERTAPTLLTPHAGEAAALLGVDREEVEAGRLAAVRELAARYGATVLLKGSTTLVAEPASGSASGSASGSASEPGAAPPVRVNPTGTPWLATAGSGDVLSGLAGSLLAAGLPARDAASAAAYLHGLSARLASRR",
    "response#2": "GPGDVITSLKRRFFENRKGDFGKLLVIGGSDKYHGAPILSAMSSIRAGVDLVIISTPELIANTIRAHTPNFIVRDYPGRYLTLDSVKYISDLINWADAIVLGPGLGVESETKEAVCKLFTLIKEANKPLVVDADALKVLAENPRILAGSPAVATPHRGEFKLLTGLEIKSSADILDNINEILSKISEFDITFAVKGAVDLVAYKNMFKLDLLGNPGMTVGGIGDVLTGVLGCFLSQGVEPFKAACAAVFITGLSGNLIAEKKGYHFTSAELIEYIPEALEEC",
    "response#3": "GPGDVITSLKRRFFENRKGDFGKLLVIGGSDKYHGAPILSAMSSIRAGVDLVIISTPELIANTIRAHTPNFIVRDYPGRYLTLDSVKYISDLINWADAIVLGPGLGVESETKEAVCKLFTLIKEANKPLVVDADALKVLAENPRILAGSPAVATPHRGEFKLLTGLEIKSSADILDNINEILSKISEFDITFAVKGAVDLVAYKNMFKLDLLGNPGMTVGGIGDVLTGVLGCFLSQGVEPFKAACAAVFITGLSGNLIAEKKGYHFTSAELIEYIPEALEEL"
  },
  {
    "reference": "MTATTKVNLLGLTQPQLEGFFESIGEKRFRAGQVMKWIHHFGVDDFEAMSNIGKALREKLRACAEIRGPEIVSQDISSDGTRKWVVRVASGSCVETVYIPQAGRGTLCVSSQAGCSLDCSFCSTGKQGFNSDLTAAEIIGQVWIANKSFGTVPAKIDRAVTNVVMMGMGEPLMNFDNVVAAMQIMMDDLGYGISKRKVTLSTSGVAPMIDELGKVIDVSLALSLHAPNDELRNRLVPINRKYPLAVVLDACRRYISRLGEKRVLTVEYTLLKDVNDQPEHAAQMIELLRDVPCKINLIPFNPFPFSGYERPSNNAIRRFQDLLHQAGHNVTVRTTRGDDIDAACGQLVGQVMDRTRRSERHIAVRQLGDEAAPSASAVNRT",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.2. The S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions.3. The protein design must be able to perform 4 iron, 4 sulfur cluster binding, rRNA binding, metal ion binding in order to contribute to rRNA base methylation.4. The S-methylcysteine intermediate, Proton acceptor of the protein must be engineered to enable effective catalysis of adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.5. The designed protein must have at least one Radical SAM core domain. The designed protein sequence is ",
    "response#1": "FKGRATVCISTQAGCAMGCVFCATGQMGLSRHLTPGEIVGQILHVQRILRETGESLRNVVLMGMGEPLHNYDNTLAAVDVLVDPLGLAMGPRFITLSTVGVVPGIRRLADEDRPVQLAVSLHGATDAERAALVPAGRRWPLAELMDACRYYIARRKRRIFFEWTLIAGRNDTSEHAHTLGALLGGMDAHVNVIPLNPTVGYDGDPSQPASVRAFQDVLMSHGVPSTVRQRRGIRK",
    "response#2": "MSDTPTNLYDLTRPALGALLSDWGFRPHHRDVLWTGLYRQQVRALDEVEGLRSDLRDTLRERTRLSHLATHHEAFSSDGHTHKLLLRLEDGQTIETVLMRFKGRATVCISTQAGCAMGCVFCATGQMGLSRHLTPGEIVGQILHVQRILRETGESLRNVVLMGMGEPLHNYDNTLAAVDVLVDPLGLAMGPRFITLSTVGVVPGIRRLADEDRPVQLAVSLHGATDAERAALVPAGRRWPLAELMDACRYYIARRKRRIFFEWTLIAGRNDTSEHAHTLGALLGGMDAHVNVIPLNPTVGYDGDPSQPASVRAFQDVLMSHGVPSTVRQRRGIDIDAGCGQLKSAVERRSRLRWRHVDPSSDTPTNLYDLTRPALGALLSDWGFRPHHRDVLWTGLYRQQVRALDEVEGLRSDLRDTLRERTRLSHLATHHEAFSSDGHTHKLLLRLEDGQTIETVLMRFKGRATVCISTQAGCAMGCVFCATGQMGLSRHLTPGEIVGQILHVQRILRETGESLRNVVLMGMGEPLHNYDNTLAAVDVLVDPLGLAMGPRFITLSTVGVVPGIRRLADEDRPVQLAVSLHGATDAERAALVPAGRRWPLAELMDACRYYIARRKRRIFFEWTLIAGRNDTSEHAHTLGALLGGMDAHVNVIPLNPTVGYDGDPSQPASVRAFQDVLMSHGVPSTVRQRRGIDIDAGCGQLKSAVERRSRR",
    "response#3": "DGDRSTICVSSQVGCALDCSFCSTARQGFNRNLTTAEIIGQVWAATRELGRPPSNVVLMGMGEPLANFDNVVRAMQLMTEDLAYMIARTRVTLSTSGIVPALRRLREASNVSLAVSLHAPDNALRDELVPINRKYPLEELIPACRDYIAGEKRRKITWEYVMLDGVNDSIAHAKALIRLLQGIPSKVNLIPFNPFPGTDYRTSPLERIDAFRQRLIRAGIITITRKTRGDDIDAACGQLVGEIVGRAGG"
  },
  {
    "reference": "MLERLTLRAIDLRAFDAIERDRVAGDVGDAAALLASVQGAVDEAFALVTCHRVELYTLASRGGTTPAAAITWNRASHRVSGLDVAEHLMAVAAGLESELVGEPEILGQIAHARAIARERGALGPVLDALATEAIRAGRAARATTGIARGPASIGAAALAVTRQHVAGPIDHALVVGAGHTGTLIAAGLRRLGVPRIDIANRSLDRAVAVARAPGRALGLDDLSAAVALATVVITATSAPSWVLDRDLVARSMARRGARPLLIVDLAVPRDTDPDIGALPGVTLIDLDAVHALAAETSRERMIDAPAVRAIVADHVAAFATRTRVLAAASTIAALHTAAETRRAAIARRASRNLSPEGRAAVDRATRQVAASLLHLPSVALRGGSGHDLGTGPMLNPRERAIRIGTRRSALARAQTDIVVELIRRARPGLTVTIVPLSSVGDRQSEAPIREIGGVGAFVTEIENALRDGRVDIAVHSLKDVPAGERPGLVFAAFPRRGDPRDALALRPDARDLGGPAPWPELPRGAIVGTGSPRRASHLRAIRPDLRLVEIRGNVDTRLTKLDRGDVDGLILAMAGLERLGRLDRVTYPIDAERLVPMVGQGALGIQCRADDWTVREILGRIDDGSTRRCVEAERAFLLAMGAGCELGVGAYARINRESMHVRAFAADGDRRATIVVDGAADDGFRLGVTAAARVIAALATPCGCFATNVEVTP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein contains novel Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase, Porphobilinogen deaminase C-terminal, Porphobilinogen deaminase N-terminal, Glutamyl-tRNA reductase N-terminal domains that confer a unique function or activity.2. The substrate, NADP(+) binding site should be stable and able to withstand changes in environmental conditions.3. The designed protein is the optimization of the Nucleophile to achieve high catalytic efficiency for H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+).4. The protein requires dipyrromethane to activate a critical catalytic site.5. Ideal protein should have a binding affinity for key enzymes in the protoporphyrin-IX biosynthesis, 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2, Porphyrin-containing compound metabolism pathway to enhance the overall efficiency of the pathway.6. For general function, the protein need meet that Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.7. A protein with NADP binding, hydroxymethylbilane synthase activity is needed to enhance protoporphyrinogen IX biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "MSIRLGTRRSQLARSQSTAIAQQLAARTGWRIEIVEVVTEGDVNMTPLARLGGSGVFVSSVRTALLTGKVDIAVHSLKDLPTAPAAGIEMAAVPPRVDPSDVLIARNGLSLVELPPGSVVGTGSPRRASQLRAARPDITVTGVRGNVGTRINHVTEGRLDGVVLAAAGVRRLGRLGEVTDFLDSSVMLPAPGQGALAVECRAPDVKLGTRRSQLARAQSTALAQQLAAQTGWRVEICEVVTEGDINMTPLAALGGTGVFVSAVRTALLTGKVDIAVHSLKDLPTAPAAGIDLAAVPPRVDPSDVLIARDGLTLAELPAGSVVGTGSPRRAAQLRAARPDIEVRGVRGNVDTRIRHVTEGRLDGVVLAAAGVRRLGRLSEVTDVLATSVMLPAPGQGALAVECRAADADIEPSLVADVTRVLAERAMNRGLDGGCQVPIGAYAELEGDEVYLRGLVGEPDGGKVLRDEVRGPASKAEELGSELAERLARAVKLGTRRSQLARAQSTALAQQLAAQTGWRVEICEVVTEGDINMTPLAALGGTGVFVSAVRTALLTGKVDIAVHSLKDLPTAPAAGIDLAAVPPRVDPSDVLIARDGLTLAELPAGSVVGTGSPRRAAQLRAARPDIEVRGVRGNVDTRIRHVTEGRLDGVVLAAAGVRRLGRLSEVTDVLATSVMLPAPGQGALAVECRAADADIEVRGLIREAMDADAQRRAANRISPTVAALREQAAAAVEAEFERVANKLGERADAAVLKEIRKSLRRTADKIIHTPTVRVKELSAEDSGVDYAAALTTLFD",
    "response#2": "LRVATRGSKLSIAQTMIALEAIKRVEPSLEYELVIVKTRGDIHQDKPFTAIGGKGLFEKEVNLAVLEGRADIAVHSLKDVPSAISPGLVLAMTPPRDPPYDVLVVRGGKEKTIWDLPSGAIVGTSSARRVAMLKHVRRDLVFKVLRGNVDTRLRKLEQGQYDAIVLAEAGLKRLGVDIEYWRIPPDILPPAPGQGIIGVYTLSSRSAEGHVGDLAGANALVVGAGAMSGLTAKTLKDHGLKRIRVINRTLDKALRLAQSVGGEAVELTQENLVREIAQADVIVSVTGARGIVLDEDTVIASLQQDLDQKLNKFFIDLALPYDIAVEVGELPHIRVLNLDGIEAVSCERGFLATLDGSCRTPIAGYAQSDGTHIRFAGMILTPDGTTSHQIEIDGRAADAERLGQEAGERIIQAEVIIDAGVQNFMHWLGQRRTVPLIQQLNAQTDEWRAAEIARAKKLIAKGEPMDAVLDALTRGLTQKMLHGALAELHAGDAASREATAHTVSRLLRARGLTAQ",
    "response#3": "MVLRIATRKSPLALKQTKYVQKKILSLYPDLNIKLVPIVTHGDNILNKSLSKIGGKGLFIKELELALLENKADIAIHSMKDLPVKITKELCLVSICKRGNALDSLVSNNYQSINQLPKGAIVGTSSLRRQCQLITYRPDLIISPLRGNIETRIAKLDQGKYDAIILATEGLNRLRLKNRITQIIPAELSLPSCGQGAIGIQSRLHDKKVKIGTRGSKLAVVQALEAKQKLLDSFPNLSIEIVKIKTSGDKYANANLAEIGGKGLFIKEIEAELLENNIDMAVHSLKDVPAFFSWGLTIPCVLERLSPCDAFISHKHNSLESLPQQATIATSAIRRKVQLLNFRPDLNIVPLRGNVTTRLQNQSFDGIILAEAGLIRLEKHHLITEVLPPKVMLSAVGQGAICIQCRRNDVKYLPSEIFDAERAFLIALGGGCNLPMAAYATATATEISIEGIFATKDGEFFARKTISGSINDKRTLARQLALALGFNARTIVNQEVDWFSVWLKNRKVAPVIEAYWNNLDDIRNEELKWLFPKLGNIDKHTEIVLKRFTHRLLRKISKPSIEAIKEIAQNLHQTDNPINTAKNL"
  },
  {
    "reference": "MSTSSNTSGECSPFSITDSIVVQATDDNADKILQEVDLSDPNIDKNDTFELRQQVSIKESPLYYLRDIPYSAYTSFQVSLIFLIVIYNGFLGPLAGNVFIPALPLLQKEFNVSETTINATVSVFMATFSISPLFWGALADKGGRKILYIISISLMVIINILLASVPKKIGSLIFLRIIQAFASSSVISLGAGTVADLTPPKDRGKAMAYFMLGPNLGPILAPIIAGLILLDNNNWRWLFGFLCIVSGLGLIMVILLLPETLRCIVGNGDRQWENWSQNENDMSTQPVDFSSPISRWSFVSDIGFLNPITQDSIFKGLYPHPPKFSVWTYLRIMTFPPVILTSIANALLFCTYYSISVTLSHFLATEYSYSNLKIGACYVCPGVCMLLGSQIGGHLSDSMRKSWKKENYNTEYPLEFRLILTVCGVLLAIGGSIGYGWCIQFHYHISAVLVFAGLMAFGLTWCNNTIMTYLSELLSLRVSSAIAVSSFFRNIAAAISSALIAKLCQKMGIGFCFLGLGLINLVSLFSILVLINNRNKWVKDSF",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be expressed specifically in the cell periphery2. The protein must exhibit the following characteristics: Plasma membrane acetic acid exporter, relieving the stress induced upon cells within hemocytes, and thus enabling increased proliferation and virulence against Galleria mellonella larvae; Confers resistance to weak acid and oxidative stress, but not to antifungal drugs..3. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "SLITAPSLTGLISIVYDLIGKVLGGSSGQSISDLSICDLLSIIDLRVSQSVLNDGIADFNGSVLLYRNYLEALDSWNKNPNSASAEELRTRFRIADSEFDRILTRGSLTNGGSLARQNAQILLLPSFASAAFFHLLLLRDATRYGTNWGLYNATPFINYQSKLVELIELYTDYCVHWYNRGFNELRQRGTSATAWLEFHRYRREMTLMVLDIVASFSSLV",
    "response#2": "ITQIPLTKVDTRGTGVSYVNDPGFIGGALLQRTDHGSLGVLRVQFPLHLRQQYRIRVRYASTTNIRLSVNGSFGTISQNLPSTMRLGEDLRYGSFAIREFNTSIRPTASPDQIRLTIEPSFIRQEVYVDRIEFIPVG",
    "response#3": "ITQIPLTKVDTRGTGVSYVNDPGFIGGALLQRTDHGSLGVLRVQFPLHLRQQYRIRVRYASTTNIRLSVNGSFGTISQNLPSTMRLGEDLRYGSFAIREFNTSIRPTASPDQIRLTIEPSFIRQEVYVDRIEFIPVL"
  },
  {
    "reference": "MKSTNISRSFSKIKKEKRIALMPFLMAGDPDLETTAKILLELQANGADMIELGIPYSDPLADGPIIQLAASRALSAGTSPDRVFKMLFELRDQLKIPIILFTYSNPLINKGLEEFCWQASKVGVSGLVVPDLPLEEAEKLSDIAESKEIDLVLLVAPTTPKDRMKKIAATSNGFTYLVSVTGVTGERSSLEDNVGSLVQQLKDSSSSPIAVGFGISDVKHIEQVREWGADGAIVGSALVKRIANASIEMKVEEAGSFCKELRAATN",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that can modulate the overall flux of metabolites through the L-tryptophan biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "PLCLGIDPHPELLRAWGLGTDAAGLAGFSDICVAAFAGFAVVKPQVAFFEAYGSAGYAVLERTIAALRESGVLVLADAKRGDIGSTMAAYASAWAGDSPLAADAVTASPYLGFGSLQPLLDTAREHDRGVFVLAATSNPEGASVQCAVAPGAIPGRTVAQAIVDAAAEVNAGAARGSVGVVVGATLSAPPDVSALNGPVLVPGVGAQGGRPEALAGLGGARRGQLLPAVSREVLRAGPDVAALRAPLCLGIDPHPELLRAWGLGTDAAGLAGFSDICVAAFAGFAVVKPQVAFFEAYGSAGYAVLERTIAALRESGVLVLADAKRGDIGSTMAAYASAWAGDSPLAADAVTASPYLGFGSLQPLLDTAREHDRGVFVLAATSNPEGASVQCAVAPGAIPGRTVAQAIVDAAAEVNAGAARGSVGVVVGATLSAPPDVSALNGPVLVPGVGAQGGRPEALAGLGGARRGQLLPAVSREVLRAGPDVAALRV",
    "response#2": "PLCLGIDPHPELLRAWGLGTDAAGLAGFSDICVAAFAGFAVVKPQVAFFEAYGSAGYAVLERTIAALRESGVLVLADAKRGDIGSTMAAYASAWAGDSPLAADAVTASPYLGFGSLQPLLDTAREHDRGVFVLAATSNPEGASVQCAVAPGAIPGRTVAQAIVDAAAEVNAGAARGSVGVVVGATLSAPPDVSALNGPVLVPGVGAQGGRPEALAGLGGARRGQLLPAVSREVLRAGPDVAALRH",
    "response#3": "PLCLGIDPHPELLRAWGLGTDAAGLAGFSDICVAAFAGFAVVKPQVAFFEAYGSAGYAVLERTIAALRESGVLVLADAKRGDIGSTMAAYASAWAGDSPLAADAVTASPYLGFGSLQPLLDTAREHDRGVFVLAATSNPEGASVQCAVAPGAIPGRTVAQAIVDAAAEVNAGAARGSVGVVVGATLSAPPDVSALNGPVLVPGVGAQGGRPEALAGLGGARRGQLLPAVSREVLRAGPDVAALRAR"
  },
  {
    "reference": "MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDASVFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADIKKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGELYRHGAEWKFKAVGQGFAGGLAALASQHGVNI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design must be focused on enhancing the efficiency of response to tellurium ion.2. For general function, the protein need meet that Not known; seems to contribute to the tellurium resistance (Ter) mechanism The designed protein sequence is ",
    "response#1": "MMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLPVRAILAELTGRRGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATKFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPR",
    "response#2": "MMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLPVRAILAELTGRRGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATKFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPL",
    "response#3": "MMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLPVRAILAELTGRRGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATKFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPF"
  },
  {
    "reference": "MGGKWSKKSTAGWTAVRERIKQAEPAAEGVGAVSRDLEKHGAITNSNTAASNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAIDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEKEEVEEANEGENNCLLHPMSQHGIDDPEREVLKWKFDSKLAFHHVAREKHPEYYKNC",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein can be expressed in extracellular region, host cell plasma membrane and perform SH3 domain binding, GTP binding without triggering undesirable cellular responses.2. The protein design must feature the Diacidic; necessary for CD4 internalization motif. The designed protein sequence is ",
    "response#1": "STCNR",
    "response#2": "MK",
    "response#3": "GMAS"
  },
  {
    "reference": "MSLALSDVKRLSMLAQIDLTDAQSAQTLDKLNGIFALVEQLSAVDTTGVEPLNHPIAALLPDLSLRLRDDVVSEPNRRDDYQQVAPATQDGLYLVPKVIE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp- tRNA(Asn) or phospho-Glu-tRNA(Gln)..2. The designed protein must possess asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity to support regulation of translational fidelity, translation under certain conditions. The designed protein sequence is ",
    "response#1": "NFFELATKECKNYRALSNWVTVEFAGRCKTQGKNLAFSGILPSGVAQLVNFIDKGVITGKIAKDIADMMMESPDKSPEVILKENPEMLPMTDESALVAIISEVISANPQSVVDYKSGKTKALGFLVGQIMKRTQGKAPPNRVNEILLVEE",
    "response#2": "NFFELATKECKNYRALSNWVTVEFAGRCKTQGKNLAFSGILPSGVAQLVNFIDKGVITGKIAKDIADMMMESPDKSPEVILKENPEMLPMTDESALVAIISEVISANPQSVVDYKSGKTKALGFLVGQIMKRTQGKAPPNRVNEILLVEAS",
    "response#3": "NFFELATKECKNYRALSNWVTVEFAGRCKTQGKNLAFSGILPSGVAQLVNFIDKGVITGKIAKDIADMMMESPDKSPEVILKENPEMLPMTDESALVAIISEVISANPQSVVDYKSGKTKALGFLVGQIMKRTQGKAPPNRVNEILLVES"
  },
  {
    "reference": "MAKLIYSYLFISMFVLSVLLALPNAEGADIKRCVVDVKLSKPCTFQECIPLCFQRYNGNGVCTGKKNEICTCAYNC",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a specific function in killing of cells of another organism and should be localized to extracellular region in order to carry out this function efficiently.2. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "GNRLGILVTTYVNAINSGAVPCLDDAVTTLAQRENSVAVQKAADHYSEQMAQRLRLPTETLQELLDVHAACEKEAMAVFMEHSFKDENQQFLKKLVELIGENKELFLSKNEEASNKYCQEELDRLSKDFMENISTFFVPCGHKLYMDKREKIEHDYWQVPRKGVKASEVFQSFLQSQAFIESSILQADTALTAGEKAIAEERAQKVAAEKEQELLRQKQKEQQEYMEAQEKSHKENLEQLRRKLEQEREQDIKDHDMMLKKLMKDQKAFLEEGFKKKAEEMNKEIQQLI",
    "response#2": "GNRLGILVTTYVNAINSGAVPCLDDAVTTLAQRENSVAVQKAADHYSEQMAQRLRLPTETLQELLDVHAACEKEAMAVFMEHSFKDENQQFLKKLVELIGENKELFLSKNEEASNKYCQEELDRLSKDFMENISTFFVPCGHKLYMDKREKIEHDYWQVPRKGVKASEVFQSFLQSQAFIESSILQADTALTAGEKAIAEERAQKVAAEKEQELLRQKQKEQQEYMEAQEKSHKENLEQLRRKLEQEREQDIKDHDMMLKKLMKDQKAFLEEGFKKKAEEMNKEIQQLV",
    "response#3": "GNRLGILVTTYVNAINSGAVPCLDDAVTTLAQRENSVAVQKAADHYSEQMAQRLRLPTETLQELLDVHAACEKEAMAVFMEHSFKDENQQFLKKLVELIGENKELFLSKNEEASNKYCQEELDRLSKDFMENISTFFVPCGHKLYMDKREKIEHDYWQVPRKGVKASEVFQSFLQSQAFIESSILQADTALTAGEKAIAEERAQKVAAEKEQELLRQKQKEQQEYMEAQEKSHKENLEQLRRKLEQEREQDIKDHDMMLKKLMKDQKAFLEEGFKKKAEEMNKEIQQLI"
  },
  {
    "reference": "MAKQRPATLSVYLYIPNIVGYMRVLLNCIAFSVCFSNKTLFSLLYFFSFCCDAVDGWCARKFNQVSTFGAVLDMVTDRVSTACLLVILSQIYRPSLVFLSLLALDIASHWLQMYSTFLSGKTSHKDVKDSTSWLFRLYYGNRMFMGYCCVSCEVLYIILLLIATNQTENLMNVVVKSLMQISPLSLLLALSIFGWSIKQIINVIQMKTAADVCVLYDIEKQHKKP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction; May thus act to reduce an excessive cellular PtdIns content; The exchange activity is due to the reverse reaction of PtdIns synthase and is dependent on CMP, which is tightly bound to the enzyme .2. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.3. A protein that can perform metal ion binding in membrane, endoplasmic reticulum, Golgi apparatus to support phosphatidylinositol biosynthetic process.4. The protein should have a Helical domain that is long enough to span the entire membrane.5. The protein have enhanced a CDP-1,2-diacyl-sn-glycerol + myo-inositol = a 1,2-diacyl-sn-   glycero-3-phospho-(1D-myo-inositol) + CMP + H(+). The designed protein sequence is ",
    "response#1": "IEKVDLARTFYFSYSYDLSQTIQYGFTHPIPQHQVRDMFVWNWNMLRPILDSVGIDSPWCIPLIHGFVDQAKLSVYGKPIIVTLIARRSRHFAGARFLRRGIRDDGYVANEVETEQIVFDGSASSFPISSTTPGIPCYTSYVQHRGSIPLRWSQEFSNITPKPPIGIDFHDPFYASTALHFDRLFGHYGIPCIVLNLVKSSEKVKRESLLLDEFESAIQYLNQFLKDSQKIQYIAWDMSAASKKKVPVTKTLEQMASDIVKKTGFFCTADRFFPGTFQTGVVRTNCVDCLDRTNAAQFVIGKCVLAAQLRALGVLDSPQLDYESDAVRLLAEMYHGHGDAIALQYGGVFPERISQGSSGSYFVKDPKGKIIGVFKPKSEEPYGHLNPKWTKYFHKVCCPCCFGRGCLIPNQGYLSEAAASLVDQKLGLWIVPKTKVVHLASETFHYNAIDRAKSRGKKYALEKVPKVGRRFHRVGLPPKVGSFQLFVEGYHEADFWLRKFEAEPLPENMRKQLQSQFERLVVLDYVIRNTDRGNDNWLIKYEKPGDGELTEKESEWTDPKDSAIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVAFSQETRDLVLSRISDMNFVQDLCEDLYEMFRTDKGFDKTMFEKQMSVMRGQILNLTQALKDGKSPIQLVQMPRVVVETTERTTPVYSPGVFPERISQGSSGSYFVKDPKGKIIGVFKPKSEEPYGHLNPKWTKYFHKVCCPCCFGRGCLIPNQGYLSEAAASLVDQKLGLWIVPKTKVVHLASETFHYNAIDRAKSRGKKYALEKVPKVGRRFHRVGLPPKVGSFQLFVEGYHEADFWLRKFEAEPLPENMRKQLQSQFERLVVLDYVIRNTDRGNDNWLIKYEKPGDGELTEKESEWTDPKDSAIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVAFSQETRDLVLSRISDMNFVQDLCEDLYEMFRTDKGFDKTMFEKQMSVMRGQILNLTQALKDGKSPIQLVQMPRVVVE",
    "response#2": "INNVLNTDGFYFCTDYDLTHTQQRLSNTSPDFQEMSLLERADQRFMWNGNLLREIIAQPELHKFAFPVIHGFIVMKPCCINGKVFEWIIISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVQFNNARASFVQTRGSIPFFWSQRPNLKYKPKPLISKDTNHMDGLRRHFESQVLIYGKQVILNLVNQKGSELPLEQAFAKMVSSMENGFIKYIAFDFHKECSKMRWHRLQILVDAVSDMQEEFGYFMVSSDGKVLSEQSGTFRSNCMDCLDRTNVIQSLLARRSLQSQLQRMGVLHVGQKIEEQADFEKIYKNAWADNANACAKQYACGVLGTIHLLSCDYLLVATHRLFVGVLNGAVVWRLAGYDIIPYIPNSFQRKENENYLRLLRQTLDTKFFYFSYRYDLTNSLQRQREVAQSRPEVSGLLQRAEQRFVWNGYVLRQFNCDKMEKFQLPLVLGFVSINQVQINGQTFFWSIITRRSVQRAGTRLFCRGSDEQGHVANFVETEQIVEFNGQLTGFVQTRGSMPFHWHQLPNLRYKPRPVLVPGKDHLAACGLHFKEQIRLYGNNVAVNLVDHKGAEGELEATYARLVREMGNPQVRYESFDFHSECPEPASRPCYAILGLFRFTAGYYLYLCTERKVVAVIGGHNVYHVDKTQFIELNPSRRHNTSVERKCMSSIEKVDLARTFYFSYSYDLSQTIQYGFTHPIPQHQVRDMFVWNWNMLRPILDSVGIDSPWCIPLIHGFVDQAKLSVYGKPIIVTLIARRSRHFAGARFLRRGIRDDGYVANEVETEQIVFDGSASSFPISSTTPGIPCYTSYVQHRGSIPLRWSQEFSNITPKPPIGIDFHDPFYASTALHFDRLFGHYGIPCIVLNLVKSSEKVKRESLLLDEFESAIQYLNQFLKDSQKIQYIAWDMSAAS",
    "response#3": "SSGSYFVKDPKGKIIGVFKPKSEEPYGHLNPKWTKYFHKVCCPCCFGRGCLIPNQGYLSEAAASLVDQKLGLWIVPKTKVVHLASETFHYNAIDRAKSRGKKYALEKVPKVGRRFHRVGLPPKVGSFQLFVEGYHEADFWLRKFEAEPLPENMRKQLQSQFERLVVLDYVIRNTDRGNDNWLIKYEKPGDGELTEKESEWTDPKDSAIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVAFSQETRDLVLSRISDMNFVQDLCEDLYEMFRTDKGFDKTMFEKQMSVMRGQIFLNISLPLWLYAILGLFRFTAGYYLYLCTERKVVAVIGGHNVYHVDKTQFIELNPSRRHNTSVERKCMSSIEKVDLARTFYFSYSYDLSQTIQYGFTHPIPQHQVRDMFVWNWNMLRPILDSVGIDSPWCIPLIHGFVDQAKLSVYGKPIIVTLIARRSRHFAGARFLRRGIRDDGYVANEVETEQIVFDGSASSFPISSTTPGIPCYTSYVQHRGSIPLRWSQEFSNITPKPPIGIDFHDPFYASTALHFDRLFGHYGIPCIVLNLVKSSEKVKRESLLLDEFESAIQYLNQFLKDSQKIQYIAWDMSAASYAILGLFRFTAGYYLYLCTERKVVAVIGGHNVYHVDKTQFIELNPSRRHNTSVERKCMSSIEKVDLARTFYFSYSYDLSQTIQYGFTHPIPQHQVRDMFVWNWNMLRPILDSVGIDSPWCIPLIHGFVDQAKLSVYGKPIIVTLIARRSRHFAGARFLRRGIRDDGYVANEVETEQIVFDGSASSFPISSTTPGIPCYTSYVQHRGSIPLRWSQEFSNITPKPPIGIDFHDPFYASTALHFDRLFGHYGIPCIVLNLVKSSEKVKRESLLLDEFESAIQYLNQFLKDSQKIQYIAWDMSAAS"
  },
  {
    "reference": "MKVYETVIGLEVHVELATKSKIFCGCTTQFGGEVNAHCCPICMGMPGTLPVLNKKVVEFAIAAGLAMNCDITKNCKFDRKNYFYPDLPKAYQVSQLYLPICRNGSIEIEVDGIKKSIGIHEIHMEEDAGKLVHDPWEDCTLVDYNRCGVPLIEIVSEPDMRSAEEVIAYLEKLKLILQYLGVSDCKMQEGSLRADINLSIREVGEPEFGTRTEMKNMNSFKAIARAIEGERKRQIELLEDGKKVIQETRRWDDNKDTSFAMRSKEDAQDYRYFPEPDLVPMEISEEWLTEIKGREPELRDAKMLRYVKEYEIPEYDAGIITGSKNLADIFEATVSLCNKPKEVSNWLMVETMRLLKESEQDAEELKLSPANFASLIELIDAGKINRTIAKEVFEQIFKANVDPNAYIEEHGLGMVSDDGVVRSTIENILKENVQSVSDYKNGKDKAFGFLVGQTMKAMRGKANPSVINEILRELLSKA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp- tRNA(Asn) or phospho-Glu-tRNA(Gln).; Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl- tRNA or glutaminyl-tRNA synthetases.2. The protein design must be able to perform glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity, ATP binding, asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity in order to contribute to translation.3. The desired protein should be able to perform ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+)   + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate The designed protein sequence is ",
    "response#1": "VIGLEVHVELNTKSKLFSSAQNRFGDEPNTNISPVCTGMPGSLPVLNKEAVNKAILFACAVQGEVALLSRFDRKSYFYPDSPRNFQITQFEHPIVRGGYVKAIVRGEERYFELAQAHIEDDAGMLKHFGEFAGVDYNRAGVPLIEIVSKPCMFCADDAVAYATALVSLLDYIGISDCNMEEGSVRFDVNVSVRPKGSEELRNKVEIKNMNSFAFMAQALEAERCRQIEAYLENPNKDPKTVIPGATYRWDPEKKKTVLMRLKERAEDYKYFIEPDLPVLQLTEAYDAFEEVAGSIDPEFAALWMRDELKRVLYYNKISFAESMITPGDIIELLAMIRKKEITSKAAKKIIEEMPLNKKGPREIATEMGLIGIIDESEVIRAVEQAIRENPGAVEDYHAGKEAAINFLVGQVMRMTRGKAEPERTVELIKERLG",
    "response#2": "VMVGLEVHVQLNNLNTKMFCGCSTQYHDSEPNTHVCPVCMGLPGALPVINKRAVESAIKIGMALNCEVVEQTQFHRKNYYYPDLPKGFQTTQYDFPIVGNGKVVIEGEDGEHVVRITRAHMEEDPGKLVHIGSIDKSKGTLIDYNRSGMALIEIVSEPDMRSPKEARRYLDKLRNILDYLDVFNGDLEGSMRVDANVSVMGGQRAEVKNISSHKGAERAILYEIMRQKNLLRRGGEVVMETRHFDEARGVTISMRTKEGEHDYRYFPEPDLVPMRVSDYFERTLTVCGDAKAASNWVMGEVMRTLKEKYLDIKEFAISPERLGGLIGLIGKGAISNTIAKQVFELMQTGEASAEEIVDREGLAQVSDTGAIERTVDEILEANPKQLADYREGKTKLFGFFVGQCMARMKGKANPQVVNDVLKSRL",
    "response#3": "MDAFEEVAGSIDPEFAALWMRDELKRVLYYNKISFAESMITPGDIIELLAMIRKKEITSKAAKKIIEEMPLNKKGPREIATEMGLIGIIDESEVIRAVEQAIRENPGAVEDYHAGKEAAINFLVGQVMRMTRGKAEPERTVELIKERYQGEAF"
  },
  {
    "reference": "MRLMISGGGTGGHIYPALALIDALKAHDPEAQVQFVGTHRGLESRIVPERGIDFKTIKIQGFKRSLSLQNVKTVYLFLKSVVTARKYIKAFKPDVVVGTGGYVSGAVVFAASQMHIPTVIHEQNSVVGVTNKFLSRFVDKIAISFESARSQFPAQKVVMTGNPRAQQVANIKKSGALAQFDLDPDIPTALIFGGSRGAARINAAAVAAIPELNKRDYQTLFVTGQVHYEKIRNGLSATALAPNVKIEPYIKNMPAILPEVAVILGRAGATSIAEITALGIPSILVPSPYVTNDHQTKNAQSLVNAGAAELIKEADLTGTSLVAALDGLLQSTTHRETMAANAKKLGMPDAADQLLHVLETVIK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be designed to localize specifically to plasma membrane in order to perform its role in cell cycle, peptidoglycan biosynthetic process, cell wall organization, regulation of cell shape, carbohydrate metabolic process more effectively.2. For general function, the protein need meet that Cell wall formation3. The protein design should consider the endogenous proteins and enzymes present in the Cell wall biogenesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "ITGTDGKTTTATLVHDIAAAEGSLRGNRAWGVGNIGVPFSAMVGEMEEGDTAVVELSSYQLERCVTFRPDVSVLTNITPDHLGRYDGSMERYAAAKFRICMNQKEGDTLIYNADDSLLEEHFGGGAFPFRRVPFSLEEPPSAGVMHDGVFLRDGEIVVARDGGERRVARTDGFLKNSFRGRHNIYNALAAVAA",
    "response#2": "LVVIGGSQGAVGLNRMVRGALPSLLEAGCRVVHLTGNNDSDVGELDHPNLVEQPFSHEMPGLLQHADLAISRAGAGSLSELAVCGTPAILVPFPQAADQHQEANAACAAALGAAVIVHQHAAEHRALGHALEQLMGPRLRGNAAASNV",
    "response#3": "VTGTSGKTTTVSMLRHLLDAPGTPAASIGTLGVLVGSEGAPLPGGAGLTTPGPVELQRLLRLLVDAGIRRVAMEVSSHALDQRRVEGVRFDAAVFTNLSRDHLDYHPSMEAYLAAKARLVSHIAPGGIAVVNDDVDTWRSLPPAPRVVRYGVSSARADVRAAGVTYTPAGSAWRLVTPAGEAPVTLPLIGDFNVANALGAAAAAAAV"
  },
  {
    "reference": "MDVSAVLQSHPVFAAFAPEALAEAVKLGTSMTYETGDICIRHGDSGEVFGVLISGRLEASRGQGTTERQFLGFVEPGECFGEMSLLTGTQSQADIVAVTHSEAVVFLQEAISPLIAGNREAVQYLTRLITRRLTSVPARPAQMAPAPPRYALGACAPIRVLAVSCRTNDLRYSYFDTTSEVALARGEVQGLGRDKAVLTHHGPGGKTETRDVAGDTHEAALAAALGVLTAPGSGLINAVSELSAIGHRVVHGGTRYNGPAVVDATVKAEIARLAPLAPLDNPLNLRGIEACERLVPGVPQIAVFDTSFHSTLPVASYRYALPKDLAKDPEIRRFGFHGISHEGAARAAAAFLGVGFDTLKMITCHLGAGASLTAIDHGRSVDNTMGATVLEGLVMATRCGDLDPGLVLHLLRHHDMTADQLEQKLYHESGLLGLSGISDDMLAVADAAEKGDAEALVATQVFCQRARKYLSAYVGLLGGVDVVLFTGGVGENAPGIRARVCQGLDWMGIALDEAQNRSARVWPRETALISASSSRAHVLVVGGDEEGTIARQAVRALSHERVTHVMRRKAQPIPIGVSAHHVHLTPEHVERLFGPGRTLTWHSDLTQPGQFACKEQVNLIGPRDRIDRVRVLGPVRPESQVEISRTEEYKLGIDAPIRLSGDLAGTPGIVLEGPAGQIRLDHGVICAQRHIHMSPAEAMEFAVRDRDVVRIRVDGDRELIFGDVVVRVRPDFRLDMHIDTDEANAAEIGKGAVGYLDSIQQRASG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a high specificity for substrate, ATP, Mg(2+) binding, with minimal off-target effects.2. The designed protein is the optimization of the Proton donor/acceptor to achieve high catalytic efficiency for acetate + ATP = acetyl phosphate + ADP.3. We require a protein that can specifically bind to the metabolites produced in the acetyl-CoA biosynthesis, Metabolic intermediate biosynthesis pathway.4. The protein must have high specificity towards ATP binding.5. The designed protein should contain one or more Cyclic nucleotide-binding domains that are essential for its function. The designed protein sequence is ",
    "response#1": "LFAGFDTVRLKELADGSGIEAFVPGERIAHAGDEVHFLGVVLEGEVLATIDDDSGPRELARFNPGDTFGEMALMSGENVLADLTAVSACKVMLIPLTLFRSHIMADAGAVQS",
    "response#2": "LFEGFDADRLTEIAAGSRIETFTPGEAVTYSGEAVHSLGVVLEGELSASVVDEVGSPRILGSLGAGETFGEMALMSGEAALADLTAAKTTRVMQVPLGLFQSHVMDEPRAVQHISRTIT",
    "response#3": "LFAGFDTVRLKELADGSGIEAFVPGERIAHAGDEVHFLGVVLEGEVLATIDDDSGPRELARFNPGDTFGEMALMSGENVLADLTAVSACKVMLIPLTLFRSHIMADAGAVQK"
  },
  {
    "reference": "MDQSMIALALFIILLVLLYKWSVAKYDVFSERGVSHEKPWPLIGNIPLKAMIGGMPVLKKMIELHTKHTGSPVYGIYALRDAVFFVRDPELIKLIGIKEFDHFVNHNSMHNNIQESILSKSLISLRDGRWKEMRNILTPAFTGSKMRIMYDLIQSCSEEGVIHIQEQLELSQDASIELEMKDYFTRFANDVIATVAFGISINSFRRKDNEFFRIGQAMSRISAWSVVKAMLYALFPRLMKVLRIQVLDTKNIDYFSSLVTAAMRYRQEHKVVRPDMIHLLMEAKQQRLADLSDKSKDELYYSEFTADDLLAQCLLFFFAGFEIISSSLCFLTHELCLNPTVQDRLYEEIISVHEELKGQPLTYDKLTKMKYLDMVVLEALRKWPPSISTDRECRQDIDLFDENGQKLFSARKGDVLQIPIFSLHHDPENFEDPEFFNPERFADGHALESRVYMPFGVGPRNCIGNRMALMELKSIVYQLLLNFKLLPAKRTSRDLLNDIRGHGLKPKNGFWLKFEARQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein can be targeted to endoplasmic reticulum membrane and perform oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, heme binding, monooxygenase activity, iron ion binding.2. For general function, the protein need meet that May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides. The designed protein sequence is ",
    "response#1": "YDGWYNNLANSEWGSAGSRLHRDARSYYSDGVYSVNNSLPSARELSDILFKGESGIPNTRGCTTLLAFFSQVVAYEIMQSNGVSCPLETLKIQVPLCDNVFDKECEGKTEIPFTRAKYDKATGNGLNSPREQINERTSWIDGSFIYGTTQPWVSSLRSFKQGRLAEGVPGYPPLNNPHIPLNNPAPPQVHRLMSPDRLFMLGDSRVNENPGLLSFGLILFRWHNYNANQIHREHPDWTDEQIFQAARRLVIASMQKIIAYDFVPGLLGEDVRLSNYTKYMPHVPPGISHAFGAAAFRFPHSIVPPAMLLRKRGNKCEFRTEVGGYPALRLCQNWWNAQDIVKEYSVDEIILGMASQIAERDDNIVVEDLRDYIFGPMHFSRLDVVASSIMRGRDNGVPPYNELRRTFGLAPKTWETMNEDFYKKHTAKVEKLKELYGGNILYLDAYVGGMLEGGENGPGELFKEIIKDQFTRIRDGDRFWFENKLNGLFTDEEVQMIHSITLRDIIKATTDIDETMLQKDVFFFKEGDPCPQPFQVNTTGLEPCVPFMQSTYWTDC",
    "response#2": "YDGWYNNLANSEWGSAGSRLHRDARSYYSDGVYSVNNSLPSARELSDILFKGESGIPNTRGCTTLLAFFSQVVAYEIMQSNGVSCPLETLKIQVPLCDNVFDKECEGKTEIPFTRAKYDKATGNGLNSPREQINERTSWIDGSFIYGTTQPWVSSLRSFKQGRLAEGVPGYPPLNNPHIPLNNPAPPQVHRLMSPDRLFMLGDSRVNENPGLLSFGLILFRWHNYNANQIHREHPDWTDEQIFQAARRLVIASMQKIIAYDFVPGLLGEDVRLSNYTKYMPHVPPGISHAFGAAAFRFPHSIVPPAMLLRKRGNKCEFRTEVGGYPALRLCQNWWNAQDIVKEYSVDEIILGMASQIAERDDNIVVEDLRDYIFGPMHFSRLDVVASSIMRGRDNGVPPYNELRRTFGLAPKTWETMNEDFYKKHTAKVEKLKELYGGNILYLDAYVGGMLEGGENGPGELFKEIIKDQFTRIRDGDRFWFENKLNGLFTDEEVQMIHSITLRDIIKATTDIDETMLQKDVFFFKEGDPCPQPFQVNTTGLEPCVPFMQSTYWTDC",
    "response#3": "YDGWYNNLANSEWGSAGSRLHRDARSYYSDGVYSVNNSLPSARELSDILFKGESGIPNTRGCTTLLAFFSQVVAYEIMQSNGVSCPLETLKIQVPLCDNVFDKECEGKTEIPFTRAKYDKATGNGLNSPREQINERTSWIDGSFIYGTTQPWVSSLRSFKQGRLAEGVPGYPPLNNPHIPLNNPAPPQVHRLMSPDRLFMLGDSRVNENPGLLSFGLILFRWHNYNANQIHREHPDWTDEQIFQAARRLVIASMQKIIAYDFVPGLLGEDVRLSNYTKYMPHVPPGISHAFGAAAFRFPHSIVPPAMLLRKRGNKCEFRTEVGGYPALRLCQNWWNAQDIVKEYSVDEIILGMASQIAERDDNIVVEDLRDYIFGPMHFSRLDVVASSIMRGRDNGVPPYNELRRTFGLAPKTWETMNEDFYKKHTAKVEKLKELYGGNILYLDAYVGGMLEGGENGPGELFKEIIKDQFTRIRDGDRFWFENKLNGLFTDEEVQMIHSITLRDIIKATTDIDETMLQKDVFFFKEGDPCPQPFQVNTTGLEPCVPFMQSTYWTDTC"
  },
  {
    "reference": "MKKISTFIITKNESARIARAINSVKNITDEVIVVDNESTDDTVHIAKTLGAQVIVKPWLGYVGQKSFAESMCVNDWVLNIDADEELSQELQDEIEYIFTSHNQDRYLAYQIKLLIMYRGDQKPRMFAPLNKCTRLYNKKFASFANTINSTTHDSVVFNKDVDFTGKIYLLNGIAYHYSGTSIEQLVNKANFYSSEQAKDLVKQGKKLSNFRLATEMIWCFLKAFFIRRYFVFGFDGFVDSIIFAFARFLRLAKLRDLSLKSQNVITSDNYINYCMDFKSLLQQKKRNRYPKK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must be able to glycosyltransferase activity. The designed protein sequence is ",
    "response#1": "LTGKTIVMPRRLVPDKQVDHAIQAFAKALPDHPGWRLRIFGDGPQMSRLRNLIQGLGLHDSVELLGPSQHMTEEWARASLTILPSQDGEAFPLVLLEAFAAGVPAVAYDIVTGPAEIIRHGEDGLLVPPNDVESLAEAISRLMGDEALLRSYGEKAV",
    "response#2": "LTGKTIVMPRRLVPDKQVDHAIQAFAKALPDHPGWRLRIFGDGPQMSRLRNLIQGLGLHDSVELLGPSQHMTEEWARASLTILPSQDGEAFPLVLLEAFAAGVPAVAYDIVTGPAEIIRHGEDGLLVPPNDVESLAEAISRLMGDEALLRSYGEKAL",
    "response#3": "LTGKTIVMPRRLVPDKQVDHAIQAFAKALPDHPGWRLRIFGDGPQMSRLRNLIQGLGLHDSVELLGPSQHMTEEWARASLTILPSQDGEAFPLVLLEAFAAGVPAVAYDIVTGPAEIIRHGEDGLLVPPNDVESLAEAISRLMGDEALLRSYGEKAP"
  },
  {
    "reference": "MTTAAIIVAAGRGTRAGGGTPKQWQKLRGKTIAARAMQPFADHQAIAQLILVVHPDDAPSLWADTPAATVVAGGATRQESVLSGLNAAEGATQVLIHDAARPMLRAAVIDAVLNALDQHPGAAPAIPVTDTLWHGADDTVVGAQDRAGLYRAQTPQGFHRAPLLAAHLACKTEATDDVTVARAAGMHVAIVAGDDDNIKITTPGDFARAEQQMGSNMDIRCGNGYDVHRFGPGDHVWLCGIKVPFDRSLQGHSDADVGMHAVTDAIYGALGMGDIGQHFPPSDPQWKGAQSHIFLNHSVALAAENGYEISNIDCTLVCEFPKIGPHQPAMKAALAQIMGLGADRISVKATTSERLGFTGRGEGIAALATATLVKP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be optimized for 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity, metal ion binding activity in order to improve isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway, terpenoid biosynthetic process.2. The protein should have a fast turnover rate for 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-   erythritol 2,4-cyclic diphosphate + CMP.3. The designed protein should have a high specificity for 4-CDP-2-C-methyl-D-erythritol 2-phosphate binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "AAAAARVGTETRSASGFDVHRLENGEELWLGGVLIPHHQGLSGHSDADVALHAITDAVLGTIAAGDIGTHFPPSDPRWRGAESGQFLAHARSLVEARGGCVTFVDLTLMCEAPKIGPHRAAMQARIAELLGLSTDRVSVKATTTERLGFTGRGEGIAAQAVATRVGSGFDVHRFAEGDHVTICGVTIPHDKGLAGHSDADVGMHALTDAILGALGEGDIGQHFPPSDAKWKDADSEIFLAHAAGLVKERQGHINHVDITLICEAPKLTPHRKAMIARIGDILGLSGDRISIKATTTERLGFTGRGEL",
    "response#2": "MTGSTAVNTGLRVGSGFDVHRFAEGDHVTICGVTIPHDKGLAGHSDADVGMHALTDAILGALGEGDIGQHFPPSDAKWKDADSEIFLAHAAGLVKERQGHINHVDITLICEAPKLTPHRKAMIARIGDILGLSGDRISIKATTTERLGFTGRGEGIAAQATATISYYD",
    "response#3": "MTGSTAVNTGLRVGSGFDVHRFAEGDHVTICGVTIPHDKGLAGHSDADVGMHALTDAILGALGEGDIGQHFPPSDAKWKDADSEIFLAHAAGLVKERQGHINHVDITLICEAPKLTPHRKAMIARIGDILGLSGDRISIKATTTERLGFTGRGEGIAAQATATRVGVGTDAHAFGGEGTLWLAGLEWPGEPALAGHSDGDAVAHAIVDALLSATGLGDIGSRFGTDRPELAGAHAEAFLAETARLVREAGWEIGNVSVQVQAQRPRFAARRAEAEAALSAALGGAPVSVSATTTDGLGFTGRAEGVQALAVALVWRVSRAG"
  },
  {
    "reference": "MEPVDPNIEPWNHPGSRPTTSCNKCYCKICCWHCQICFLKKGLGISYGRKKRKHRRRTPQSSEDHQNPIPKQPLPTTRGNQTGPKESKKKVASEAKTDPSD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: In the cytoplasm, Tat is thought to act as a translational activator of HIV-1 mRNAs..2. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.3. A protein that contributes to negative regulation of peptidyl-threonine phosphorylation by functioning in extracellular region with actinin binding, protein serine/threonine phosphatase inhibitor activity. The designed protein sequence is ",
    "response#1": "XGXAXGXR",
    "response#2": "MXRXGGXXX",
    "response#3": "MR"
  },
  {
    "reference": "MIDIRAIREDPEAIVERLALRHDGSAAMIAEVLTCDEARRRAETEKQKLQSERKKTSKEIGALRSRGEDSSAIEAEVKKIGERIKELESEGAEAETRQTELLLNIPNLPHEAIPRGEGEDENPELRTWGQKPEIXDPRDHVELAEQHGLISFEDGVRVAGSGFPVFRGXGARLQRALIQYLLDXQTREHGYEEVGVPYVVKRACGIGTGQLPKFAEEVYPTENDELFLIPTAEVPVTNLYRETLLQEGDLPIKMTGHTPCWRREAGSAGRDTRGIIRVHQFDKVXLVQVVHPDTSFDALEELTGHAERILQSLGLHYRVIELCGGDIGFGAARTYDIEVWAPGHGKYLEVSSCSNFTDYQARRLKLRFKDSEGRNRFCHTLNGSGTALPRLYVAILEQYQQADGSISIPEPLVPYFGADKIG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be able to compete with endogenous proteins or inhibitors for L-serine, ATP binding.2. The designed protein must have at least one Aminoacyl-transfer RNA synthetases class-II family profile domain.3. The protein must exhibit the following characteristics: Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec).; Catalyzes the attachment of serine to tRNA(Ser).4. A protein that is capable of catalyzing reactions within the selenocysteinyl-tRNA(Sec) biosynthesis, Aminoacyl-tRNA biosynthesis pathway with high efficiency and specificity.5. A protein that contributes to selenocysteinyl-tRNA(Sec) biosynthetic process, selenocysteine biosynthetic process, seryl-tRNA aminoacylation by functioning in cytoplasm with ATP binding, serine-tRNA ligase activity.6. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "MFDVKLLRTDLPGVQQAMRNRGSETAELDRFTDLDRKRRELLQETEQLKSTRNKVSQEVAIRKKNKENADDLIQEMKIVGDKIKSLDEEIRNLETDLNSLLLSIPNMPDESVPVGEDEHDNREERRWGEPRQFDFPPRDHVALGTAAGWLDFDAAAKISGARFVVLHGVLARLQRALIQFMLDVHTREHGYCEVYVPYLVNADSLYGTGQLPKFAADLFKIPGEREFYLIPTAEVPVTNLLRDTITEAVNLPRRWVAHTPCFRSEAGAYGKDTRGMIRQHQFEKVELVTAVQPEDSPAALENLTGHAESILQRLELPYRVVSLCAGDLGFSAAKTYDLEVWLPGQQCYREISSCSNFGDFQARRLQARWRNPATGKPELLHTLNGSGLAVGRTLVAVLENYQQADGSVIIPTALRPYMGGIEKIS",
    "response#2": "MLDMKMIRQNAEWFKQKMADRGVDGASVDHLMELDQKRRDLIVKSEALKAERNEVSEEISQLKRNKENADAQIKRMREVGAEIKQLDADLETTKAEQDNLEAHLPNIPDASVPDGKDETRLQRALIQFMLDVHTREHGYCEVYVPYLVNADSLYGTGQLPKFAADLFKIPGEREFYLIPTAEVPVTNLLRDTITEAVNLPRRWVAHTPCFRSEAGAYGKDTRGMIRQHQFEKVELVTAVQPEDSPAALENLTGHAESILQRLELPYRVVSLCAGDLGFSAAKTYDLEVWLPGQQCYREISSCSNFGDFQARRLQARWRNPATGKPELLHTLNGSGLAVGRTLVAVLENYQQADGSVIIDNQEISRWGEPRRFDFEVRDHVTLGEMAKGLDFAAAVKLTGSRFVVMQGQIARLHRALSQFMLDLHTEQHGYSENYVPYLVNHATLYGTGQLPKFAGDLFHTRPLEEEADSSTYALIPTAEVPLTNLVRDEILDDDALPLKMTAHTPCFRSEAGSYGRDTRGLIRMHQFDKVEMVQIVRPEESMEALEELTAHAEKVLQLLNLPYRKVLLCTGDMGFGAVKTYDLEVWLPAQNTYREISSCSNMGDFQARRMQARCRSKSDKKTRLVHTLNGSGLAVGRTLVAVLENYQQADGRIEVPEVLRPYMGCEVLKV",
    "response#3": "MLDMKMIRQNAEWFKQKMADRGVDGASVDHLMELDQKRRDLIVKSEALKAERNEVSEEISQLKRNKENADAQIKRMREVGAEIKQLDADLETTKAEQDNLEAHLPNIPHPQTPIGKTEDDNREERRWGEPRQFDFPPRDHVALGTAAGWLDFDAAAKISGARFVVLHGVLARLQRALIQFMLDVHTREHGYCEVYVPYLVNADSLYGTGQLPKFAADLFKIPGEREFYLIPTAEVPVTNLLRDTITEAVNLPRRWVAHTPCFRSEAGAYGKDTRGMIRQHQFEKVELVTAVQPEDSPAALENLTGHAESILQRLELPYRVVSLCAGDLGFSAAKTYDLEVWLPGQQCYREISSCSNFGDFQARRLQARWRNPATGKPELLHTLNGSGLAVGRTLVAVLENYQQADGSVIIPTALRPYMGGLDVIGK"
  },
  {
    "reference": "MSKINLKDKTLSELKDIFINLNEKSFRAKQVYKWIHQKNINLISDITVLSKDLRDKLEKDYELSNMTILKRLDSKQDKTKKYLFLLKDKNIIESVMMKHKHGVTLCLSTQVGCRMGCEFCASTKDGLIRNLTAGEILDQVYQIENDLNIDVSNIVLMGSGEPLDNYENVLKFLKMINSKDGHNIGFRHITLSTCGVVGKIYELANENIPITLSISLHSPYNDERKKIIPIAKKYNIEELINACKYYIEKTNRRISFEYTLIEGVNDRKKDAYKIAEIIKGMLAHVNLIPLNDINETNFNKSNNKNVIKFKNILLEKGINATVRKEMGSDINAACGQLRRDYLRNKDN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The presence of Radical SAM core domains is critical for the interaction of the designed protein with its intended targets.2. The designed protein should have a binding site that is specific to the target [4Fe-4S] cluster, S-adenosyl-L-methionine ligand.3. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.4. The protein's S-methylcysteine intermediate, Proton acceptor must be carefully engineered to ensure that the amino acids involved in adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine are properly positioned for optimal catalysis.5. The presence of [4Fe-4S] cluster is necessary for the protein to function in the desired environment.6. The protein can be targeted to cytoplasm and perform 4 iron, 4 sulfur cluster binding, tRNA (adenine-C2-)-methyltransferase activity, rRNA (adenine-C2-)-methyltransferase activity, rRNA binding, tRNA binding. The designed protein sequence is ",
    "response#1": "NSVCVTTQVGCRMGCTFCASTVGGLVRNLTAGEIVDQIVMMQRELPQGERISTVVLMGSGEPLENYDHVLKAVRLVHDPEGLNIGYRHITISTSGIVPGMRRLAEEGLPITLALSLHAPTDELRRQLMPVARIWPLAEVLAAAREYGEKTGRRVTYEYILIEGVNDGPEEARQLARLLKGALAHVNLIPMNPVAERPQYRRPGPERVNRFKESRNGVAWNKNSNPSIYSLELHELEDWLKEHGEKSFRANQVFEWLYTKRATSFEDMSNLSKSLRTLLNENFTLTTLNTLIQQTSKDGTIKFLFELHDGYSIETVLMRHEYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVKVQQALDEFEERVSHVVIMGIGEPFDNYDEMMSFLKIINHDKGLNIGARHITVSTSGIIPKIYKFADEKLQINFAISLHAPNTELRTKLMPINKAYKLPDLMEAVRYYVDKTGRRVSFEYGLFGGENDQVEHAEELAQLVKGLKCHINLIPVNYVPERNYVRTPKEQIKLFEDTLKKHGVNVTTRREQGHDIDAACGQLRAKERKEEE",
    "response#2": "AEKRITGCLSSQVGCRFSCAFCASGSKGFTRNLSSGQILDEVVYLKRCAPKRLLTHVVFMGMGEPFDNYENVLKAVRIINADYGFHIGARRITISTCGIPEGIRRLARENLQVELSVSLHAVDDATRSKLMPVNRKYPLNELFAAIGEYVKTTNRQVTFEYVVVDGINNGLNDATKLQTLLKGLNCKVNLIPYNPVEASRLKAPSLGNIKEFKGFLERRGITVTVRKSRGQD",
    "response#3": "LPDIKSMPLPELTQAIISLGEPKFRAKQLYEWMHKKMAGSYDEMTNIPKSLKEKLKASYSYTSLKVVDVQESKLDETKKFLFGLSDGNVIESVFMKYKFGCSVCISSQVGCRMGCRFCASTIDGVVRSLMPGEMLDQIYAISRITGQKISHVVVMGSGEPLDNYDNLLVFIRMLTGEDGMNMSQRNLTVSTCGLVPKIRELAGEHFSINLALSLHASNQEKRQKLMPIANKYELSDVIDACRYYFNETGRQLTFEYSLVAGVNDTTEDAQELSELLKGLNAIVNLIPVNPIKERDFAAPTRENALAFKNKLEKSGINVTIRREMGRDIDGACGQLRRRHLE"
  },
  {
    "reference": "MVSKRRVLREMVVKLFFQREFRHNEFEEIFSETLNKIRDNTIKADFKRYVEGVFHNLSTIDNIISNHLINWSFDRLSYLERNVLRVGTYELIYEENIPIEVTINEMIEIAKKYGSEESGKFVNGILDRIAKEHAPKEKFNL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons.; Involved in transcription antitermination.2. The designed protein must be optimized for RNA binding. The designed protein sequence is ",
    "response#1": "MKILYAYRFQNNEYDIIEFLNKFKELNPDENFKEIDEEYLKKLLTGVIRNQQLIDNLIEKYSKDWPLSRIPMVELELMRIAIYELLFEEEIPISVAIDEAVDLSSIFGVEKAPSFVNGILGSIV",
    "response#2": "MKILYAYRFQNNEYDIIEFLNKFKELNPDENFKEIDEEYLKKLLTGVIRNQQLIDNLIEKYSKDWPLSRIPMVELELMRIAIYELLFEEEIPISVAIDEAVDLSSIFGVEKAPSFVNGILGSIL",
    "response#3": "MKILYAYRFQNNEYDIIEFLNKFKELNPDENFKEIDEEYLKKLLTGVIRNQQLIDNLIEKYSKDWPLSRIPMVELELMRIAIYELLFEEEIPISVAIDEAVDLSSIFGVEKAPSFVNGILGSIL"
  },
  {
    "reference": "MLEGKKILIGITGSIAAYKSILLVRLLVKQGAEVKVMMTPAAKEFVSPLVLSTLSRNAVIVDLAAGNEWANHVMLGRWADVCVMAPLSCNTLAKMAHGICDNLLLAVYLSATCPVVIAPAMDEDMWQHPATQKNIALLKEYGNHLIPVNNGELASGLFGEGRMAEPEAIITYLIAHFFRGDELKGKKALVTAGPTYEAIDPVRFIGNHSSGKMGFALAEELYLRGAEVELITGPTQQKTAFAGIRVTRVVSAAEMFAASTAAFPTVDIAVMSAAVADYTPEQAATEKIKKQSGNLALELVPTKDILQHLGSEKKKGQYLVGFALETQNEKENARKKLVSKNADCIVLNSLRDEAAGFGVDTNKITLLDKAGTEMVSELKSKKEIAKDIVSFIVARTHAE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that is capable of catalyzing reactions within the Cofactor biosynthesis, coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway with high efficiency and specificity.2. A protein with metal ion binding, phosphopantothenate--cysteine ligase activity, FMN binding is needed to enhance pantothenate catabolic process, coenzyme A biosynthetic process in a specific context.3. The designed protein should have a binding site that is specific to the target CTP ligand.4. We need a protein that can perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2 with high efficiency.5. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.6. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..7. The designed protein must have at least one Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domain. The designed protein sequence is ",
    "response#1": "MKIVLGVAGGIAAYKACSLLRLLTEDGHDVTVVPTQAALRFVGAPTWAALSGRPVATEVWDAVHEVRHVRLGQEADLVVVAPATADLLARAAHGLADDLLTNVLLTASGPVVLAPAMHTEMWEHAATQANVAILSERGVHIVPPASGRLTGADTGPGRLPEPEELYAVLTRLTGDGPLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHVLGADGSEVAVADGSKREVADRVWDLVVARLR",
    "response#2": "MKIVLGVAGGIAAYKACSLLRLLTEDGHDVTVVPTQAALRFVGAPTWAALSGRPVATEVWDAVHEVRHVRLGQEADLVVVAPATADLLARAAHGLADDLLTNVLLTASGPVVLAPAMHTEMWEHAATQANVAILSERGVHIVPPASGRLTGADTGPGRLPEPEELYAVLADAATILTDPKLRGLKVVVSAGPTYEDIDPVRFIGNRSSGRMGFAVAEAAAQAGAEVTLVAGPVSIATPPGIARRIDVRSAAQMRDAVVTASAGADIYIAAAAVGDYRPAEVADHKLKKSDGAPLELRLTENPDILVTLAAQTVRPFLVGFAAETHDVERYAQDKLKRKGLDMIAANQVGGGLGFEAADNALTILSAEGVEKIPRVAKSALARAIVTHIATTLASA",
    "response#3": "MKIVLGVAGGIAAYKACSLLRLLTEDGHDVTVVPTQAALRFVGAPTWAALSGRPVATEVWDAVHEVRHVRLGQEADLVVVAPATADLLARAAHGLADDLLTNVLLTASGPVVLAPAMHTEMWEHAATQANVAILSERGVHIVPPASGRLTGADTGPGRLPEPEELYAVLEAEARLLLDGLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHVLDREGGVAALAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHFLAADGTVEELPPLSKRAVADRVLDRAAALVGARG"
  },
  {
    "reference": "MSKEKFKRSKPHINVGTIGHVDHGKTTLTSAITTVLSKKYGGAACAFDQIDNAPEEKARGITINTSHVEYDTSIRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHILLGRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLTQYDFPGDSTPIVRGSALKALEGDPQWEQKILDLSNYLDTYIPEPKRSIDQPFLLPIEDVFSISGRGTVVTGRIERGIIKVGEEVEIVGIKSTVKTICTGVEMFRKLLDEGRAGENVGVLLRGTKRDDIERGQVLAKPGTITPHIKFESEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGSVELPEDMEMVMPGDNVKMVITLIHPIAMSDGLRFAIREGGKTVGAGIVVKVLQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be able to perform GTP binding under specific conditions. The designed protein sequence is ",
    "response#1": "RKELTTTEMTQHLNAYYQECGRNIGRQIPLIIQYSILQTFGQEMEKAMLQLLQDTSKCNWFLTEQSDSREKKKFLKRRLLRLDEAQRKLAKV",
    "response#2": "ADVGLVGFPNAGKSTLLSKVSAAKPEIADYAFTTLAPNLGVVKYRDYRSFIMADIPGIIEGAAEGKGLGIRFLRHIERNSILLFLIAADSKDIRAEYEILLNELQKYNPELLHKDRILAISKSDMLDDELKAELKKELPKGVETVFFSSYLNQGLTELKDLLWTTMV",
    "response#3": "MIPVIALVGRPNVGKSTLFNQLTRSRDALVADYPGLTRDRKYGDGKLGEHEFIVIDTGGISGDEQGIDEKMARQSLLAIEEADVVLFLVDGRHGLNPADEMIASHLRRSNKQVSLVVNKTDGINEDIALADFYSLGFGELHPIAASHGKGVHVLIDKVMLPFAERVEEAKNQIVIALVGRPNVGKSTLFNQLTRSRDALVADYPGLTRDRKYGDGKLGEHEFIVIDTGGISGDEQGIDEKMARQSLLAIEEADVVLFLVDGRHGLNPADEMIASHLRRSNKQVSLVVNKTDGINEDIALADFYSLGFGELHPIAASHGKGVHVLIDLR"
  },
  {
    "reference": "MSTMFADTLLIVFISVCTALLAEGITWVLVYRTDKYKRLKAEVEKQSKKLEKKKETITESAGRQQKKKIERQEEKLKNNNRDLSMVRMKSMFAIGFCFTALMGMFNSIFDGRVVAKLPFTPLSYIQGLSHRNLLGDDTTDCSFIFLYILCTMSIRQNIQKILGLAPSRAATKQAGGFLGPPPPSGKFS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be optimized for stability and solubility when expressed in endoplasmic reticulum where it will be involved in ER overload response, calcium ion transmembrane transport.2. For general function, the protein need meet that Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes; Within the MPT complex, the GEL subcomplex may mediate insertion of transmembrane regions into the membrane.3. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells. The designed protein sequence is ",
    "response#1": "SGDQILIRTTQIYTYFVYKTRNMDMKRLIMVLAGASEFDPQYNKDATSRPTDNILIPQLIREIGSINLKKNEPPLTCPYSLKARVLLLSHLARMKIPETLEEDQQFMLKKCPALLQEMVNVICQLIVMARNREEREFRAPTLASLENCMKLSQMAVQGLQQFKSPLLQLPHIEEDNLRRVSNHKKYKIKTIQDLVSLKESDRHTLLHFLEDEKYEEVMAVLGSFPYVTMDIKSQVLDDEDSNNITVGSLVTVLVKLTRQTMAEVFEKEQSICAAEEQPAEDGQGETNKNRTKGGWQQKSKGPKKTAKSKKKKPLKKKPTPVLLPQSKQQKQKQANGVVGNEAAVKEDEEEVSDKGSDSEEEETNRDSQSEKDDGSDRDSDREQDEKQNKDDEAEWQELQQSIQRKERALLETKSKITHPVYSLYFPEEKQEWWWLYIADRKEQTLISMPYHVCTLKDTEEVELKFPAPGKPGNYQYTVFLRSDSYMGLDQIKPLKA",
    "response#2": "SGDQILIRTTQIYTYFVYKTRNMDMKRLIMVLAGASEFDPQYNKDATSRPTDNILIPQLIREIGSINLKKNEPPLTCPYSLKARVLLLSHLARMKIPETLEEDQQFMLKKCPALLQEMVNVICQLIVMARNREEREFRAPTLASLENCMKLSQMAVQGLQQFKSPLLQLPHIEEDNLRRVSNHKKYKIKTIQDLVSLKESDRHTLLHFLEDEKYEEVMAVLGSFPYVTMDIKSQVLDDEDSNNITVGSLVTVLVKLTRQTMAEVFEKEQSICAAEEQPAEDGQGETNKNRTKGGWQQKSKGPKKTAKSKKKKPLKKKPTPVLLPQSKQQKQKQANGVVGNEAAVKEDEEEVSDKGSDSEEEETNRDSQSEKDDGSDRDSDREQDEKQNKDDEAEWQELQQSIQRKERALLETKSKITHPVYSLYFPEEKQEWWWLYIADRKEQTLISMPYHVCTLKDTEEVELKFPAPGKPGNYQYTVFLRSDSYMGLDQIKPLKH",
    "response#3": "SGDQILIRTTQIYTYFVYKTRNMDMKRLIMVLAGASEFDPQYNKDATSRPTDNILIPQLIREIGSINLKKNEPPLTCPYSLKARVLLLSHLARMKIPETLEEDQQFMLKKCPALLQEMVNVICQLIVMARNREEREFRAPTLASLENCMKLSQMAVQGLQQFKSPLLQLPHIEEDNLRRVSNHKKYKIKTIQDLVSLKESDRHTLLHFLEDEKYEEVMAVLGSFPYVTMDIKSQVLDDEDSNNITVGSLVTVLVKLTRQTMAEVFEKEQSICAAEEQPAEDGQGETNKNRTKGGWQQKSKGPKKTAKSKKKKPLKKKPTPVLLPQSKQQKQKQANGVVGNEAAVKEDEEEVSDKGSDSEEEETNRDSQSEKDDGSDRDSDREQDEKQNKDDEAEWQELQQSIQRKERALLETKSKITHPVYSLYFPEEKQEWWWLYIADRKEQTLISMPYHVCTLKDTEEVELKFPAPGKPGNYQYTVFLRSDSYMGLDQIKPLKV"
  },
  {
    "reference": "MENGTGDEQNHTGLLLSSQEFVTAEYQVVTILLVLLICGLGIVGNIMVVLVVLRTKHMRTPTNCYLVSLAVADLMVLVAAGLPNITESLYKSWVYGYVGCLCITYLQYLGINASSFSITAFTIERYIAICHPIKAQFLCTFSRAKKIIIFVWSFASVYCMLWFFLLDLNIAVYKDTTVVSCGYKVSRSYYSPIYMMDFGIFYVLPMVLATVLYGLIARILFLNPIPSDPKENSNTWKNDMAQQNKTVNSKMTNKSFNSTIASRRQVTKMLAVVVVLFAFLWMPYRTLVVVNSFLSSPFQENWFLLFCRICIYLNSAINPVIYNLMSQKFRAAFRKLCNCHLKRDKKPANYSVALNYNVIKESDHFSSEIEDITVTNTYLSSAKTSIGDTCLSSEA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be able to perform thyrotropin-releasing hormone receptor activity under specific conditions.2. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells. The designed protein sequence is ",
    "response#1": "FDGWYNSLAHPERGAVGAHLVRLVPARYWDGVYQPVHEPVQPNARGLSRLLAGGASGLSSARNRTVLSLFFGYHVAFEIFDVRTPGCPPQFMNIKVPKGDPVFDPNATGNVRLPFQRGQWNKLSGQSPGNPRAQVLTLYFLFTFNQVNSVTAWIDGSSIYGPSSSWSDFLRSFSGGLLKRGSEWNMPNKGGGRNFMWSAPDPSTGEYGPQGLYELGNAWANENLFTAAEGIIWFRYHNYVAAKLHKKNPEWSDERLFQNARKTVVATLQNIALYEWLPGFLGDRKLPPYPAGYQKFVDPGISPEFQAAAMRFGVTMAPPGVYMRNKTCHFRKITNIDGSKSPAIRLCNSFWKRQSANMKTSQDVDELLMGMASQIAEKEDHIVVEDLRDFMYGPLRFSRTDLVALTIQRGRDFGLQSYTETRRALDLPPVKTFEEINPELSSSNPQVSPAVSEKMNSWTTKTVQLLRDVAELYGGDILKLELFPGGLLESTDGPGPVFSAIILDQFERVRNGDRFWFENKQNGLGCFLSLETKYLLFLQCSNAFLLFSLFTDEEIQTIRRLTYRDVLIAVTSAEEADLQENVFLWTEGDPCPQPAQLDASMLHPCTNVTNLDYFDGH",
    "response#2": "FDGWYNSLAHPERGAVGAHLVRLVPARYWDGVYQPVHEPVQPNARGLSRLLAGGASGLSSARNRTVLSLFFGYHVAFEIFDVRTPGCPPQFMNIKVPKGDPVFDPNATGNVRLPFQRGQWNKLSGQSPGNPRAQVLTLYFLFTFNQVNSVTAWIDGSSIYGPSSSWSDFLRSFSGGLLKRGSEWNMPNKGGGRNFMWSAPDPSTGEYGPQGLYELGNAWANENLFTAAEGIIWFRYHNYVAAKLHKKNPEWSDERLFQNARKTVVATLQNIALYEWLPGFLGDRKLPPYPAGYQKFVDPGISPEFQAAAMRFGVTMAPPGVYMRNKTCHFRKITNIDGSKSPAIRLCNSFWKRQSANMKTSQDVDELLMGMASQIAEKEDHIVVEDLRDFMYGPLRFSRTDLVALTIQRGRDFGLQSYTETRRALDLPPVKTFEEINPELSSSNPQVSPAVSEKMNSWTTKTVQLLRDVAELYGGDILKLELFPGGLLESTDGPGPVFSAIILDQFERVRNGDRFWFENKQNGLGCFLSLETKYLLFLQCSNAFLLFSLFTDEEIQTIRRLTYRDVLIAVTSAEEADLQENVFLWTEGDPCPQPAQLDASMLHPCTNVTNLDYFDGQ",
    "response#3": "WSQFMKHGEDLLNQTISTAVKEIALAHLNGFKDVLTYYERAFNDWKRNPSANTARLVSQRFENAHFNFVSNMPQLQLPTYDTLLLSCYTEAANLHLNLLHQGVQFADQWNADQPHSPMLKSSGTYYDELLVYIEKYINYCTKTYHKGLNHLKESEKITWDAYNTYRREMTLIVLDLVATFPFYS"
  },
  {
    "reference": "MDIREMDLKRQEANALFKKQMIDEALEIYKTSFLEATKSVVPGTRNDLLEEQLSLLAYNISVVYYKRKNFPKSLAFGLESLKHRKSDKVLCKICAIYLRLGMLREYKEMYDQMVTRSSGPEVAFLLKRMKLSEVIVEKHLEKRVTLESLHELSKEISGGRSIPADTLESILEQGESILLGCENVVHTESSGEVLIFGDTHGQYFDVVSILNKVFDKDRMVIFNGDYVDRGSHSVENFALLLSLKILFPGRVHLTRGNHELSDINRVYGFYDEVKRKYPFSSDSVYRRFQDAFRALPISIIVNEKVFITHGGLPEAPVKVDNLQEIYRMTDTHTDELLKGLLWSDPEEILGTEESKRRAGVVFGADVTARFLERNGLDLLVRSHQAVDDGYRVHHGGKVVTIFSAPEYEGSKGPGSYLVLNPSAGEADEIVEISPLTRYKAVKFGRSDGKEVLRLLCN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The Proton donor of the protein must be engineered to enable effective catalysis of H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +   phosphate.2. Mn(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "DCTENDDDGDEDGNEDSDEEETDEDQMANDNFCANMIEEPGKDSGCTAVVCLLQGRDLYVANAGDSRCVISRSGQAIEMSIDHKPEDDEEASRIIKAGGRVTLDGRVNGGLNLSRALGDHAYKTNVTLPAEEQMISALPDIKKLIITPEDEFMVLACDGIWNYMSSEEVVEFVRCRLKDNKKLSTICEELFDNCLAPNTC",
    "response#2": "DCTENDDDGDEDGNEDSDEEETDEDQMANDNFCANMIEEPGKDSGCTAVVCLLQGRDLYVANAGDSRCVISRSGQAIEMSIDHKPEDDEEASRIIKAGGRVTLDGRVNGGLNLSRALGDHAYKTNVTLPAEEQMISALPDIKKLIITPEDEFMVLACDGIWNYMSSEEVVEFVRCRLKDNKKLSTICEELFDNCLAPNTC",
    "response#3": "QMANDNFCANMIEEPGKDSGCTAVVCLLQGRDLYVANAGDSRCVISRSGQAIEMSIDHKPEDDEEASRIIKAGGRVTLDGRVNGGLNLSRALGDHAYKTNVTLPAEEQMISALPDIKKLIITPEDEFMVLACDGIWNYMSSEEVVEFVRCRLKDNKKLSTICEELFDNCLAPNTMGDGTGCDNMTAVIVQF"
  },
  {
    "reference": "AVIPLKNQGKCGSCWAFSTVTTVESINQIRTGNLISLSEQQLVDCSKKNHGCKGGYFDRAYQYIIANGGIDTEANYPYKAFQGPCRAAKKVVRIDGCKGVPQCNENALKNAVASQPSVVAIDASSKQFQHYKSGIFTGPCGTKLNHGVVIVGYGKDYWIVRNSWGRHWGEQGYTRMKRVGGCGL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The protein should be able to bind E64 ligand in a variety of conditions3. The protein must exhibit the following characteristics: Cysteine proteinase..4. The target protein must exhibit strand-helix-turn-helix-turn-helix-turn-helix-strand-helix-strand-strand-strand-strand as its primary conformation.5. The ideal protein would be one that plays a critical role in proteolysis through cysteine-type peptidase activity within extracellular region. The designed protein sequence is ",
    "response#1": "MGKNKLLYPSLTLLLLLLLPTDASVSGKPQYMVLVPSLLHTETPEKGCLLLSHLNETVTVSAA",
    "response#2": "VPSELDLRSLRTVTPIRMQGGCGSCWAFSGVAATESAYLAYRNTSLDLSEQELVDCASQHGCHGDTIPRGIEYIQQNGVVEERSYPYVAREQRCRRPNSQHYGISNYCQIYPPDVKQIREALTQTHTAIAVIIGIKDLRAFQHYDGRTIIQHDNGYQPNYHAVNIVGYGSTQGDDYWIVRNSWDTTWGDSGYGYFQAGNNLMMIEQYPYVVQ",
    "response#3": "IPEYVDWRQKGAVTPVKNQGSCGSCWAFSAVVTIEGIIKIRTGNLNEYSEQELLDCDRRSYGCNGGYPWSALQLVAQYGIHYRNTYPYEGVQRYCRSREKGPYAAKTDGVRQVQPYNEGALLYSIANQPVSVVLEAAGKDFQLYRGGIFVGPCGNKVDHAVAAVGYGPNYILIKNSWGTGWGENGYIRIKRGTGNSYGVCGLYTSSFYS"
  },
  {
    "reference": "MKRALISVSNKEGLLDFARGLEKLGYEIISTGGTYNTIKEAGIKVKKVADITGFPEILDGRVKTLHPKIHGGILARRIPEHLQQLQENDIGPIDIVAVNLYPFRETISRPGVSLEEAIENIDIGGPAMVRAAAKNYQDVAVVVKPEFYQALLEALEKPGGISPEFRLKLALEAFSHTAAYDAMISSYLSRVSNSDLNQNLILAGEKVYDLRYGENPHQKASFYRFMTPGLGLPDARQLNGKELSYNNIIDTQAAWELVREFDEPACVIIKHTNPCGTAVAATLEEAFDRAFAADPLSAFGGIIALNRPVDGATAQKAAEPFMEVIIAPAYTPEALESLQAKKNLRVLELPLEVAGGLQIRTVAGGFVVQESDRDKLDMDKVQVVTQKEPTVEQLKELAFARKVVKHIKSNAIVVARDGMTLGVGAGQMNRVGSARIALESAGEKARGAVMASDAFFPFKDTVELAAQYGITAIIQPGGSVRDQESIDECDKHGIAMVFTGIRHFKH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must be optimized for IMP cyclohydrolase activity, phosphoribosylaminoimidazolecarboxamide formyltransferase activity.2. Ideal protein should have a binding affinity for key enzymes in the Purine metabolism pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "LLREVATKIIKISDMDIDKIVTAEAMGIPIATTISLYTDIPYVIMRKRKYLLEGEIPVHQETGYSKGELYLNGVNKGDKVLIVDDVISTGGTMVAIIKALEKAGAHIKDIVCVIERGNGKEEVKEKTGYDVKTLVL",
    "response#2": "LLREVATKIIKISDMDIDKIVTAEAMGIPIATTISLYTDIPYVIMRKRKYLLEGEIPVHQETGYSKGELYLNGVNKGDKVLIVDDVISTGGTMVAIIKALEKAGAHIKDIVCVIERGNGKEEVKEKTGYDVKTLVL",
    "response#3": "LLREVATKIIKISDMDIDKIVTAEAMGIPIATTISLYTDIPYVIMRKRKYLLEGEIPVHQETGYSKGELYLNGVNKGDKVLIVDDVISTGGTMVAIIKALEKAGAHIKDIVCVIERGNGKEEVKEKTGYDVKTLVL"
  },
  {
    "reference": "MNEADVTKFVNNARKTLTDAQLLCSSANLRIVDIKKKLSSWQLSISKLNFLIVGLRQQGKFLYTILKEGIGTKLIQKQWNQAVLVVLVDEMKYWQYEITSKVQRLDGIVNELSISEKDDTDPSKLGDYISRDNVNLLNDKLKEVPVIERQIENIKLQYENMVRKVNKELIDTKLTDVTQKFQSKFGIDNLMETNVAEQFSRELTDLEKDLAEIMNSLTQHFDKTLLLQDKKIDNDEREELFKVVQGDDKELYNIFKTLHEVIDDVDKTILNLGQFLQAKIKEKTELHSEVSEIINDFNRNLEYLLIFKDISNLIDSFKNSCTQDIQTTKELCEFYDNFEESYGNLVLEAKRRKDVANRMKTILKDCEKQLQNLDAQDQEERQNFIAENGTYLPETIWPGKIDDFSSLYTLNYNVKNP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be involved in response to starvation by localizing to vacuole-isolation membrane contact site and exhibiting protein kinase activator activity, molecular adaptor activity. The designed protein sequence is ",
    "response#1": "LNCKNHNVLSNYNVANKDILLKNELDTPPSKTSEMWYLAPCEDGSRRQRPSECSSDDLMCAIRHVKVDGSDHLTQKLDVHKSAKYEVEEVESGGFDIRFLGVKWGSNTIDATLSYSCDTNLKTDELVTTIWNSEYVLIEMSGPSGCKY",
    "response#2": "APHPPINEQSNSSQPFYRVSPSIVPLNVIRKLTNRKTSFTDSHILRLQDEVNQLRNELDLVNKRNEDLLIELQGKEEKA",
    "response#3": "VPEDCKVFKSSLSPLKITFKTKDARKYSLMFKVGDDLRQDQFVVQIITLMNALLKNENVDLKLTPYRILATGHNEGAIEFIPNDTMANILSKHHGILPFLKSNFPDRGEPLGVQDWVMDNFVKSCAGYCVITYILGVGDRHLDNLLIAPDGRFFHADFGYILGRDPKPFPPLMKLPPQIIK"
  },
  {
    "reference": "MTLAILAVFSVALTLLGFVLPPQGVKRATLLGLALALASLLLTWGKPFAFGPYAVDGVSQVFTLLALLGALWTVGLVRSGRFEFYLLVLYAALGMHLLASTRHLLLMLVALEALSLPLYALATWRRGQGLEAALKYFLLGALAAAFFLYGAALFYGATGSLVLGAPGEGPLYALALGLLLVGLGFKAALAPFHFWTPDVYQGSPTPVVLFMATSVKAAAFAALLRVAAPPEALALLVALSVVVGNLAALAQKEAKRLLAYSSIAHAGYMALALYTGNAQALGFYLLTYVLATGLAFAVLSQISPDRVPLEALRGLYRKDPLLGLAFLVAMLSLLGLPPLAGFWGKYLAFAEAARAGAWGVLVLALVTSAVSAYYYLGLGLAVFARPEETPFRPGPPWARAAVVAAGVLLLALGLLPGLVLPALAAGG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The engineered protein is expected to adopt a helix-turn-helix-turn-strand-helix-helix-helix-helix-helix-helix-helix-helix-helix-turn-based conformation.2. A protein that can perform NADH dehydrogenase (ubiquinone) activity, quinone binding in plasma membrane to support ATP synthesis coupled electron transport. The designed protein sequence is ",
    "response#1": "PVMFIGWEGVGLASFLLIGFWYKNPQYADSARKAFIVNRIGDLGFMLGMAILWALYGTLSISELKEAMEGPLKNPDLLALAGLLLFLGAVGKSAQIPLMVWLPDAMAGPTPVSALIHAATMVTAGVYLIARSSFLYSVLPDVSYAIAVVGLLTAAYGALSAFGQTDIKKIVAYSTISQLGYMFLAAGVGAYWVALFHVFTHAFFKALLFLASGSVIHALGGEQDVRKMGGLWKHLPQTRWHALIGALALGGLPLLSGFWSKDAILAATLSAA",
    "response#2": "PVMFIGWEGVGLASFLLIGFWYKNPQYADSARKAFIVNRIGDLGFMLGMAILWALYGTLSISELKEAMEGPLKNPDLLALAGLLLFLGAVGKSAQIPLMVWLPDAMAGPTPVSALIHAATMVTAGVYLIARSSFLYSVLPDVSYAIAVVGLLTAAYGALSAFGQTDIKKIVAYSTISQLGYMFLAAGVGAYWVALFHVFTHAFFKALLFLASGSVIHALGGEQDVRKMGGLWKHLPQTRWHALIGALALGGLPLLSGFWSKDAILAATLSALNY",
    "response#3": "PVMFIGWEGVGLASFLLIGFWYKNPQYADSARKAFIVNRIGDLGFMLGMAILWALYGTLSISELKEAMEGPLKNPDLLALAGLLLFLGAVGKSAQIPLMVWLPDAMAGPTPVSALIHAATMVTAGVYLIARSSFLYSVLPDVSYAIAVVGLLTAAYGALSAFGQTDIKKIVAYSTISQLGYMFLAAGVGAYWVALFHVFTHAFFKALLFLASGSVIHALGGEQDVRKMGGLWKHLPQTRWHALIGALALGGLPLLSGFWSKDAILAATLSALNY"
  },
  {
    "reference": "MKLLVATGNQGKLKEIRRLLDESGVEVIGLDQYPGAPEVIEDGDTFEANARKKALEMAEYSNCLTLADDSGLVVDALKGAPGVHSARFAGEQGNDAANNAKLLKELADVADDKRQAAFNCVMALATPKGDCVTYTGAVFGLILREQRGEGGFGYDPLFMVPEYGKTMAELPLDIKNRISHRGNALRQVIPVLQEMAVKDA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a binding site that is specific to the target Mg(2+), substrate ligand.2. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.3. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for dITP + H2O = dIMP + diphosphate + H(+).4. A protein with ITP diphosphatase activity, XTP diphosphatase activity, ribonucleoside triphosphate phosphatase activity, dITP diphosphatase activity is needed to enhance purine nucleoside triphosphate catabolic process, nucleotide metabolic process in a specific context.5. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP The designed protein sequence is ",
    "response#1": "MAYSQLVLATRNAKKLQEMQAILSPFCQGLTLIGLEDYPLFPEVPETGQTFRENALAKSVEASKATGYPTLADDSGLCVNALGGLPGVLSARWSGQHGNDQANLDLLLAQLRDVPAAQRKAQFICVMVYIRHPDDPTPLIASGQWAGQLIREPRGEGGFGYDPLFLVPALNCTSAQLDPEVKNALSHRGQALRQLLAQLKAL",
    "response#2": "MACSTYVVATGNAGKIAELRDMLAPFGVDCVSQGALGLPEPEETGRTFIENALIKARHAARVSGLPALADDSGLCVDVLGGAPGIFSARFAGEPKSDERNNALLLAQMRDVPDERRGARFVCVLALVRHADDPEPLIAEGRWFGEILEAPTGNGGFGYDPLFWLPELGKTSAELAPDEKDAISHRGRALQALAARLGPEWAAAWRAAP",
    "response#3": "MIPVVLASGNAGKLREFSALLSPLEIVPQTQFDVPEADETGLSFVENALIKARHAARKTGLPALSDDSGIVVDALGGAPGIYSARYAGEPKSDERNNAKLLAELHGIPDEKRTAHFYCALVYVRHANDPQPIICEGSWYGRILHAPRGEGGFGYDPLFHVPEHGCSAAELAPEVKNAHSHRGAALRQLFEALRAT"
  },
  {
    "reference": "MSKFIFVTGGVVSSLGKGIAVASIGKMLKSRGLTVSVMKLDPYLNVDPGTMSPYQHGEVFVTDDGSETDLDLGHYERFIDVELTCNSNITAGQIYSEVISKERSGEYLGGTIQTIPHVTNLIRDKIINLSKESDADIVIVEVGGTVGDIEGQPFLEAIRQVRNTVGRDDTFYVHLTLLPYLGASNELKTKPTQHSVNTLRSMGIQPDAILCRADFPIGEDERKKISLYCDVRYEAVIDLPTLSSVYQVPLHLESQGLGKILMDYFEYDQMSPDFKSWEKITIIQQKNNPVVEIALVGKYVELKDSYLSVVEAINHAAIMHKREAKISWISAEDIETSDIDSFLSKAQGIIIPGGFGDRGLEGMIKTAEYARTQNIPYLGLCLGMQIMVIEYARNVLKINDANSLEIDPETKNPVIAFMPGQEEVHSTGGTMRLGAYPCILKENSLAMKVYTESSISERHRHRYEFNNKFREEFESNGLLISGTSPDNKLVEITEVMNHPYMIGSQFHPEFKSRPERPHPLFTLFIENSMRIKREGDQHNLF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that can modulate the overall flux of metabolites through the CTP biosynthesis via de novo pathway pathway, enhancing or suppressing its activity as needed.2. The protein must exhibit CTP synthase activity.3. The protein's Nucleophile; for glutamine hydrolysis, Nucleophile, active site must be carefully engineered to ensure that the amino acids involved in H2O + L-glutamine = L-glutamate + NH4(+) are properly positioned for optimal catalysis.4. The designed protein should have a high specificity for L-glutamine, ATP, Mg(2+), CTP binding, with minimal off-target effects.5. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates..6. The designed protein must have at least one CTP synthase N-terminal domain. The designed protein sequence is ",
    "response#1": "KYIFITGGVVSSLGKGLSAASIGALLESRGLNISFVKLDPYINVDPGTMSPFQHGEVYVTHDGAETDLDLGHYERFTSVHMSRANNFTTGQIYETVIQKERRGEYLGRTVQVIPHVTDEIKGKILALAKNVDVLIVEIGGTVGDIEGLPFLEAIRQLKLEVGGGNAINIHVTLVPFIVAAGEVKTKPTQHSVKELREIGIQPEIILCRTDRDLTLDIKKKISLFCNVKVESVITCRDVSSIYELPLLLHREGLDERIVEALNIWTKAPDLRKWEEVTIGVVGKYTGMKDAYKSLTEALVHGGIANQVKVNIRWLDAEEFENDDVAARLEPMHGILVPGGFGERGSEGKIASVRFARERDVPFFGICLGMQMACIEAARNQAGVSGAGTTEFGPTDEPVVGMITEWMSADGLQVRQAGGNMGGTMRLGAYEALLAGNSVAASVYGAPTVSERHRHRYEVNVHYRERLEASGLIFSGMSPDGLLPEIVERPDHPWFVGVQFHPELKSKPFDPHPLFAGFVKAAIDYKRTH",
    "response#2": "KHIFVTGGVVSSLGKGITAASLGRLLKARGVKVTIQKLDPYLNVDPGTMSPFQHGEVFVTDDGGETDLDLGHYERFVDESLSRDCNVTAGSVYQTLIAKERRGDFLGGTVQVIPHVTNEIKERINRLSEQTQAEVIITEVGGTVGDIESLPFLEAIRQLKKDKGRDNVAYIHVTLVPWIAASSELKTKPTQHSVKELRSIGITPDFIVCRSDRPIDASIRRKIALFCDVDPKAVVSAIDARSIYEVPENLRAQQLDEMVIDRLQVRIALVGKYIQLHDAYLSVSESLFHAAAANDAVCDIKWVDSEELTQENIDDVLGDCEGILVPGGFGDRGIEGMILAARYAREKDVPYLGICLGMQIAVIEFARNVVGWADANSAEFTSSSLHPVIDLMPEQQGITAKGGTMRLGQYPCALDKESKAYELYGEETVYERHRHRYELNNDFREELCEKGLRLAGLSPDGRLVEVVENPANRWFVAAQFHPEFKSRPNKPQPLFYGFIHSACQQSKVNIALVGKYVQLPDAYLSVNEALDHAGIFHDNKVRVQWVDAESLTPEEVDSTLREFDGILVPGGFGIRGVEGKIRAARFARENGVPYFGICLGMQVAVAEFARHVAGLDGANSSEFDPVTEHPVIDLMRDQQDVADMGGTMRLGSYPCSVTPGTKGWEAYGDEVIYERHRHRYEVNNAYRQRLVDAGLVISGMSPDGKLVEMVELPDHPWFLGNQGHPEFKSRPTRPAPLFRDFIQAALQYK",
    "response#3": "KHIFVTGGVVSSLGKGITAASLGRLLKARGVKVTIQKLDPYLNVDPGTMSPFQHGEVFVTDDGGETDLDLGHYERFVDESLSRDCNVTAGSVYQTLIAKERRGDFLGGTVQVIPHVTNEIKERINRLSEQTQAEVIITEVGGTVGDIESLPFLEAIRQLKKDKGRDNVAYIHVTLVPWIAASSELKTKPTQHSVKELRSIGITPDFIVCRSDRPIDASIRRKIALFCDVDPKAVVSAIDARSIYEVPENLRAQQLDEMVIDRLQLQDAYLSVIEALRHAGIHHRAAIEIKWIRAGDLENTDDLASYFDGVDGILVPGGFGGRGMEGKIKAIRWAREKGIPFLGICLGMQLMAVEFARHVCGLEGAHSAEFDGETRHPIAHLLPEGKMRLGAYPVVLQPGSKAYEAYGQREIAERHRHRYVFNTDYREWFTRHGMAITGMSSDGQVVEVLELPEHPWFVGTQFHPEFKSRPNRPHPLFVGFIDAAVARKDAYKSLTEALVHGGIANQVKVNIRWLDAEEFENDDVAARLEPMHGILVPGGFGERGSEGKIASVRFARERDVPFFGICLGMQMACIEAARNQAGVSGAGTTEFGPTDEPVVGMITEWMSADGLQVRQAGGNMGGTMRLGAYEALLAGNSVAASVYGAPTVSERHRHRYEVNVHYRERLEASGLIFSGMSPDGLLPEIVERPDHPWFVGVQFHPELKSKPFDPHPLFAGFVNDAYLSVNEALKHAGYAADDKVNIHMIDAGTIDKDNVAKKLKGMDGILVPGGFGNRGVEGMIQAVRYAREKDIPYLGVCLGMQIATIEYARDVVGYKDANSTEFDPQTKHNVIDLMADQNDVTDAGGTQRLGAYPTKLKPGTKAASAYDNKKEISERHRHRYEFNNKFRKDLEDAGLVVSGTSPDDHLVEVVEVPHKKFFVAAQYHPEFLSRPDRPEGLFKAFV"
  },
  {
    "reference": "MIIFRKSFFTFWLLLNSVLALVITQNRVDRGTLDLSVGDITINSGASWSIINNAISTLVGSLTVQPNAGLYITSTSPLLSLQVTLTSLLSTIQNNGIIAFNSSPSLTSSTYNLVGLSLVNTGEMYFSASGVLPSVMALTAASWSNSGLMAFYQNQRSSGVVSLGTPLGSITNNGQICLNNEVYQQTTSINGSGCFTANRDSTIYIANVLLPVSTSQNFYLADSQSSIIVQAILTPQVFNVYGFGNGNMVGVTLPLLGNILNPAYSYNPSTGILRLRNLLVYQDFNIGPGYNPSLFSIVTDNGAGLPSTILGSVSYSGPVPPRALPASCKIACKPVPTAPGTNPTEYTTTITTTNSAGKPLTETGVVDISTDSNGSWFSSTTIFPTSSSSSSSSSTVSSTAPSSSSTKPSSSSQPSSTPPPSSSSKASSTTPSSSSQSSSTTPSSSSKPSSTVPPTGSSQSSSTIPSSSTQPSSTAPSSLSSPSSSTTPSSSSQSSFSAQSSIGQTSSSTVSSSSSQPSSSQPSSSQSSSATTSSSSQFSSSAPPSSTQSSFTAESSNSQLSSTTPSSSTEASSTVPSSSSQLSSSVPLTNSVSLSSVSSSDNGSSSASSPSSSQSSIASTAESSSTFPSSSDQQSSSIQSPSSQESSVSSTPTSSLQSSTNTISSSQDSSSFSPTTSDNSSTNSASSLSTLSSSDTSVSNPSTSNVSSTDNTQSSVASATPTDSAISATSSDITTEFTTTWEVTNSDGSVSTESGIVSESGTSFTTIT",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be expressed specifically in the cell surface2. For general function, the protein need meet that GPI-anchored cell wall protein involved in cell wall organization, hyphal growth, as well as in host-fungal interaction and virulence. The designed protein sequence is ",
    "response#1": "SNIAVYYGQGVNQPRLAEFCAETSYDIINIGFINSFPEQNPLTGLPGSDFGNQCWADTFVVDGIASQLYSHCPNIAEDIPKCQAAGKKVFLSLGGATPTYWFDTIDASTKLADFLWGAFGPVTDAWTVADKPRPFGNAVVDGFDFDIEFFGSKGYANMIKRFRRRFGEVPDQTFYISAAPQCSIPDEQLSVAIKNAVIDFVWVQFYNTPGCSARDFVLGTKNGFNYDSWVEVIKAGANPNAKLYVGLPASGAAANLGYYLTPEEVKPLVKKYMDKYPETFGGVMLWEATQARNNQIDGVGYNEKIRC",
    "response#2": "SNIAVYYGQGVNQPRLAEFCAETSYDIINIGFINSFPEQNPLTGLPGSDFGNQCWADTFVVDGIASQLYSHCPNIAEDIPKCQAAGKKVFLSLGGATPTYWFDTIDASTKLADFLWGAFGPVTDAWTVADKPRPFGNAVVDGFDFDIEFFGSKGYANMIKRFRRRFGEVPDQTFYISAAPQCSIPDEQLSVAIKNAVIDFVWVQFYNTPGCSARDFVLGTKNGFNYDSWVEVIKAGANPNAKLYVGLPASGAAANLGYYLTPEEVKPLVKKYMDKYPETFGGVMLWEATQARNNQIDGVGYNEKIRC",
    "response#3": "SNIAVYYGQGVNQPRLAEFCAETSYDIINIGFINSFPEQNPLTGLPGSDFGNQCWADTFVVDGIASQLYSHCPNIAEDIPKCQAAGKKVFLSLGGATPTYWFDTIDASTKLADFLWGAFGPVTDAWTVADKPRPFGNAVVDGFDFDIEFFGSKGYANMIKRFRRRFGEVPDQTFYISAAPQCSIPDEQLSVAIKNAVIDFVWVQFYNTPGCSARDFVLGTKNGFNYDSWVEVIKAGANPNAKLYVGLPASGAAANLGYYLTPEEVKPLVKKYMDKYPETFGGVMLWEATQARNNQIDGVGYNEKIRAC"
  },
  {
    "reference": "MAFRRINNLELFIKRCSAPTLYYSSIRTATGNNYSNNTANITTDSSSSSNNNSNRNNKNDNNNEDNNKFNWRSLVRFFVPFSLGAVASSLVMKRDDLELTPTISAARPSGRRREFNFIADVVGGCADSVVYIEIKDTRHFDYFSGQPITASNGSGFVIEQNGLILTNAHVVINKPHTMVQVRLSDGRTFPATIEDVDQTSDLATLRIQVNNLPVMRLGKSSTLRSGEWVVALGSPLALSNTVTAGVISSTQRASQELGLRNRDINYLQTDAAITFGNSGGPLVNLDGEAIGVNSMKVTAGISFAIPIDYVKVFLERAAERRKKGSAYKTGYPVKRYMGITMLTLTPDILFELKSRSQNMPSNLMHGVLVWKVIVGSPAHSGGLQPGDIVTHINKKEIKNSSDVYDALGCGDKELNMIIMRGVKQMHVTITPEDP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein is the optimization of the Charge relay system to achieve high catalytic efficiency for Cleavage of non-polar aliphatic amino-acids at the P1   position, with a preference for Val, Ile and Met. At the P2 and P3   positions, Arg is selected most strongly with a secondary preference   for other hydrophilic residues..2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.3. The presence of PDZ domains is critical for the interaction of the designed protein with its intended targets.4. The protein should be designed to localize specifically to mitochondrial intermembrane space, cytosol, Nebenkern in order to perform its role in apoptotic process, spermatogenesis, positive regulation of cysteine-type endopeptidase activity, positive regulation of apoptotic process, mitochondrion organization more effectively.5. The protein must exhibit the following characteristics: Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase- independent and serine protease activity-dependent mechanism; Serine protease that shows proteolytic activity against a non-specific substrate beta-casein; Can antagonize antiapoptotic activity of th by directly inducing the degradation of th ..6. The protein design must feature the IAP-binding motif. The designed protein sequence is ",
    "response#1": "MKMRLPTRSDMICGYACLKGNAAMRNTKRGSWYIEALTQVFSERACDMHVADMLVKVNALIKEREGYAPGTEFHRCKEMSEYCSTLCQQLYLFEPVKVIVGSPAHSGGLQPGDIVTHINKKEIKNSSDVYDALADNSKHLDIVILDCLAHLGEKVSQELKEPLHKALQMLLSQPVTYQAFRECTLETTVHASGWNKILVPLVLLRQMLLELTRRGQEPLSALLQFGVTYLEDYSAEYIIQQGGGGAGEGREKDGVSAGGRQETLKKNRVVLAKQLLLSELLEHLLEKDIITLEMRELIQAKGGSFSQNVELLNLLPKRGPQAFDAFCEALRETRQGHLEDLEDPLGPDEHCSPNQAYRLQSQPRGLALVLSNVHFTGEKDLEFRSGGDVDHTTLVTLFKLLGYNVHVLHDQTAQEMQEKLQNFAQLPAHRVTDSCVVALLSHGVEGGIYGVDGKLLQLQEVFRLFDNANCPSLQNKPKMFFIQACRGDETDRGVDQQDGKNHTQSPGCEESDAGKEELMKMRLPTRSDMICGYACLKGNAAMRNTKRGSWYIEALTQVFSERACDMHVADMLVKVNALIKEREGYAPGTEFHRCKEMSEYCSTLCQQLYLFE",
    "response#2": "LRRAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGPASPGGPGGTGTARGLALVLSNVHFTGEKDLEFRSGGDVDHTTLVTLFKLLGYNVHVLHDQTAQEMQEKLQNFAQLPAHRVTDSCVVALLSHGVEGGIYGVDGKLLQLQEVFRLFDNANCPSLQNKPKMFFIQACRGDETDRGVDQQDGKNHTQSPGCEESDAGKEELMKMRLPTRSDMICGYACLKGNAAMRNTKRGSWYIEALTQVFSERACDMHVADMLVKVNALIKEREGYAPGTEFHRCKEMSEYCSTLCQQLFELKSRSQNMPSNLTHGVLVWKVIVGSPAHSGGLQPGDIVTHINKKEIKNSSDVYDALADNSKTLDIVILRGRGAYRLQSQPRGLALVLSNVHFTGEKDLEFRSGGDVDHTTLVTLFKLLGYNVHVLHDQTAQEMQEKLQNFAQLPAHRVTDSCVVALLSHGVEGGIYGVDGKLLQLQEVFRLFDNANCPSLQNKPKMFFIQACRGDETDRGVDQQDGKNHTQSPGCEESDAGKEELMKMRLPTRSDMICGYACLKGNAAMRNTKRGSWYIEALTQVFSERACDMHVADMLVKVNALIKEREGYAPGTEFHRCKEMSEYCSTLCQQLYLL",
    "response#3": "MKMRLPTRSDMICGYACLKGNAAMRNTKRGSWYIEALTQVFSERACDMHVADMLVKVNALIKEREGYAPGTEFHRCKEMSEYCSTLCQQLYLFNHLEDEHVEDGKEESSEQEKTEGRKKASSQPCTPRKKTSKRKSEEVEPASEETPAVKEEDEADSQPKASTPAKKTKPRGLALVLSNVHFTGEKDLEFRSGGDVDHTTLVTLFKLLGYNVHVLHDQTAQEMQEKLQNFAQLPAHRVTDSCVVALLSHGVEGGIYGVDGKLLQLQEVFRLFDNANCPSLQNKPKMFFIQACRGDAYRLQSQPRGLALVLSNVHFTGEKDLEFRSGGDVDHTTLVTLFKLLGYNVHVLHDQTAQEMQEKLQNFAQLPAHRVTDSCVVALLSHGVEGGIYGVDGKLLQLQEVFRLFDNANCPSLQNKPKMFFIQACRGDETDRGVDQQDGKNHTQSPGCEESDAGKEELMKMRLPTRSDMICGYACLKGNAAMRNTKRGSWYIEALTQVFSERACDMHVADMLVKVNALIKEREGYAPGTEFHRCKEMSEYCSTLCQQLYLEKPL"
  },
  {
    "reference": "MTKYIFITGGVVSSLGKGIIGASLGKLLRARGYSVAIQKFDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDTNFSQLSNVTSGSVYQTVIKKERRGDYLGATVQVIPHITNEIKSRLVKAQKHFKPDFQIIEIGGTIGDIEGLPFIESVRQFKTEAGIGNAINIHCTLLPYLPTSGELKTKPSQHSVSLLRSYGLQPEILVCRTSKPIPKTEKDKLALFCSVSKEAVIECRDMKSIYEVPLALEEQKFADVVLKLLQVPERKADLTGWRELVDKINHPSGTIKIAIAGKYTKLSDAYISVVESIKHAGYADNVKTEIKWINSEDCVDGGKCKELLSDVDGVVVPGGFGIRGIEGKLNVIKYARENNIPFLGICLGMQCAVIEYARNAAGLKGANSTEFDENTAVPVIDLMTEQKNIKGYGGTMRLGAYDCNLKKGTKAQEVYSANKISERHRHRYEVNTDYIEQLKKAGLVFSGMSPDGMLSEIVELPHLDWFVACQFHPEFKSRPERPHPLFKGLIDAVMKQKSAKA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should contain one or more Glutamine amidotransferase domains that are essential for its function.2. The protein must have high specificity towards metal ion binding.3. The Nucleophile; for glutamine hydrolysis active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.4. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "LQDAYKSINEALFQAATYNNRRLKLVSIHSEKVNDDNVESLLKDMDGVIVAPGFGQRGIEGKFSALKWCREHDKPTFGICLGMQCMVIEYARNVLGLKDANSTEMNPQTPYNVIDLMEEQKSISNMGGTMRLGAYDCVLKAGSKAAEAYGSTHISERHRHRFEFNEEFRKQFEDAGMKCVGENPDTHLVEVVEIPEKKWYIGTQYHPEYSSTVLSPNPLFLSFVYSSVAR",
    "response#2": "LPDAYLSVTEALRAGGFAELTKVNIHWVRSDDCETEQGALSNLGEMDAICVPGGFGVRGIEGKLGALKFARENQIPTLGLCLGLQCMVIEFARNVAGITDATSSEFDPEGKNPVIATMAEQVDKIASSNLGGTMRLGLYEANLLPGSVVSKTYGSVLASERHRHRYEVNNSYREQLASAGLVFSGTSEKDSLVEYVELPADVHPYYVSTQAHPEFKSRPTKPNPLFHGLVFSESV",
    "response#3": "LPDAYLSVTEALRAGGFAHSAKTKIRWVASDLCASEEGAAKALEGVDAICVPGGFGVRGLDGKIGALRHARVHGIPALGLCLGLQTMVIEYARNVLGMSEASSTEFDPETAYPVIATMAEQEAIVEGAGDLGGTMRLGSYEAKLTEGSVVAETYGSTEITERHRHRYEVNNTYREQLTEAGLVFSGTSPDGTLVEFVELPRQTHPYYVSTQAHPELKSRPTNAHPLFAGLIS"
  },
  {
    "reference": "MSSQQAVAAVVVLAAGQGTRMRSATPKVLHELGGRSMLGHVLAAAQPLGAATTVVVVGSGREAVEAHLAAVAPGARAVLQAEQLGSGHAARVALDAVPELEGPVLVVNGDAPLLRTATLTALVDGHTAAASVFTVLTAEVDDPTGLGRIVRDDAGAPRAIVEERDATPGQRAVREVNAGVYVADAGTLRRVLAGLATDNAQGEQYLTDALAPLVEGGAPVAAVPAPDPDDVLGCNDRRELAVRRRTLNDRVLDELMRSGVTVVDPATTWVDVTVTVAPDALLEPGVQLRGTTSVATGAVVGPDTTLVDTTVGEGAAVVRSHVLGAVVGPRATVGPFSYVRPGTRLGADAKVGAFVETKNAQVGEGSKVPHLSYVGDATIGRGSNIGAATVFVNYDGVAKHHTTVGDAVRIGSDTMLVAPVTVGDGAYTAAGSVITEDVPPGAMGVARAAQRNVAGWVRRRRPGTAAADAAAAAGAAAEGTAGSRPAGDTPAGPAEPGADHER",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should contain one or more MobA-like NTP transferase domains that are essential for its function.2. The protein design should aim to mimic or enhance the function of natural proteins involved in peptidoglycan biosynthetic process, UDP-N-acetylglucosamine biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.3. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.4. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain..5. We require a protein that can specifically bind to the metabolites produced in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway. The designed protein sequence is ",
    "response#1": "VVILAAGKGERMVSKKPKVMHEIMGKPMVSYVVQRAKELLPTKIVVVLGHGRELVEGYVKKFGVECAVQTEQKGTAHALLMAEELIQGDDVLVLYGDVPLIENSTLDGLLAFYEKARAVVFMTTEVENPFGYGRIIEENGKFLRIVEEKDASDDEKSISEINSGIYCFKSKILFEALEKVSNDNVKGEYYLTDIVKISVEKGLKVNSLVANDFMEIEGVNDRVQLSEMEKIFKKNIANKLLENGVTIVDPAATYIEPGVRIGRDTVIYPGAQIGGATEVGSGCTIESNAVIRGCRLGNGVHVKTGSIMTDAVLHDAVAVGPMAHLRPGTELMAHVKIGNFVETKKIVMGEGSKASHLTYLGDATIGSNVNVGCGTITCNYDGVNKHQTVIGDDVFVGSDVQFVAPVTIGRNSLIAAGTTVTQDVPPDSLAIARTPQKNIEGWVEKNRKNS",
    "response#2": "VIVLAAGAGTRMKSSTPKVMHPIGGRSLLHHAVDAAAGVNPDHLVVVLRHERDRVGEHVANIAQARECAVLVADQDDVPGTGRAVECALTQLPQNLTGTIVVTYGDVPLLEADTIHDLVVFHEEHSNAVTVLSAHVKQPRGYGRIVRDATGTVIVLAAGAGTRMKSSTPKVMHPIGGRSLLHHAVDAAAGVNPDHLVVVLRHERDRVGEHVANIAQARECAVLVADQDDVPGTGRAVECALTQLPQNLTGTIVVTYGDVPLLEADTIHDLVVFHEEHSNAVTVLSAHVKQPRGYGRIVRDATGTRGFASRVLADDGTVVAIVEEKDASEAQRAVSEVNSGIYAFDLGPLRSGLARLGTANSQDEKYLTDVIGLLRAAGQRVAAHVVADPGLVAGVNDRVQLAEARRLLNDRLLAASMRA",
    "response#3": "VVLAAGKGVRMRSERPKVLHGIGGRSMLGHVLATAREAGAARLALVVAPGMEAVRAEAAAAAPGIEVFEQAAQLGTAHAVLSARPALERHAGDVIVLYADTPLLEAATLRRMIEALEAGAHIAALGFEAEDPTGYGRIVRDAAGRVLRIVEQKDATPEEREIGEINSGILAFDAPFLREALGRLSCDNAQGEYYLTDVLGIARAEGVAVVAVETRDPEEVEGANDRVQLAAMARRLNARVCEAWMRAGVTIVDPAATYIEPGVRIGRDTVIYPGAQIGGATEVGSGCTIESNAVIRGCRLGNGVHVKTGSIMTDAVLHDAVAVGPMAHLRPGTELMAHVKIGNFVETKKIVMGEGSKASHLTYLGDATIGSNVNVGCGTITCNYDGVNKHQTVIGDDVFVGSDVQFVAPVTIGRNSLIAAGTTVTQDVPPDSLAIARTPQRNLEGWVARRRAGTPAARAAERARAARAKPGGEP"
  },
  {
    "reference": "MFGTPSSSPSFGTPSSTPAFGTSSPAFGTPSATPAFGTPSNPSFSSGGFGSSLFSSPFSSQQPQQQQQQQQQQQPSSLFQQQPSSNFGFQSPFNNTAQQQQQTPFPNAQLTTQMAPVAPIPYSLADRDVQAIIEAYKEDPTNPKYAFQHLLFSVTEPQYRVKPAAVSDIMWAEAMSKLEGMDSTERERLWPQLVQGFKDLSQRLKLQDEVLVSDRDRIKTTQSNVKMLQRHLQASTFPSIERLRQKEQSLQRRMLRVMRIIEGLEGKGFRLPLTKGEAELSEKLTAITRQVKGPGAELSRRVQSLQTISRAQANSIAAGSSLYLPGSTKIDEQSLIDMQEVLQQETEAIGRLGNVLKRDMRDMEIMVAEDTEMALDS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be designed to localize specifically to nucleolus, nuclear pore central transport channel in order to perform its role in nuclear pore organization, NLS-bearing protein import into nucleus, protein localization to nuclear inner membrane, mRNA transport more effectively. The designed protein sequence is ",
    "response#1": "WAPFHVVRHHNIPDKVFEHLNAGEVFTKLGLFAEIGYAWASIDSSLFLWDYTHPNPELIGYEEATHTITAVALVPPKPGVFVKTITHVLVVATTSEIILLGVSATPTPSGSKSLTLYSTRMSVHRGGSDVSFIVGTKDGRIFLGGESDTDIHEIFYQQEERWFSSRCGKINHSHPGWSAVVPSLAGLPFGSRQQEWLRGLYVDDTRNLLYSLSNRSTIRTYHMEGPEKLTKVIEKDKTSCLRDFAHMADSSPLFTDKTNIVALSPIPATEASKLHLMALTDTGCRLFLSATSSASYTMGGATSLAPQSMQLQFVKFPPRESPTRIRTLNGQIIDSQLDKTSRALDPSALGFRFSPGYFFDVVRKHPNQDMLFVSAPDTGRIKVTQPASALKYFEQGTWIELENGNRTIEIGLTTAPFAAAKQPLGFGNELAVQFDQVPGEFAVLTNTGVHR",
    "response#2": "WAPFHVVRHHNIPDKVFEHLNAGEVFTKLGLFAEIGYAWASIDSSLFLWDYTHPNPELIGYEEATHTITAVALVPPKPGVFVKTITHVLVVATTSEIILLGVSATPTPSGSKSLTLYSTRMSVHRGGSDVSFIVGTKDGRIFLGGESDTDIHEIFYQQEERWFSSRCGKINHSHPGWSAVVPSLAGLPFGSRQQEWLRGLYVDDTRNLLYSLSNRSTIRTYHMEGPEKLTKVIEKDKTSCLRDFAHMADSSPLFTDKTNIVALSPIPATEASKLHLMALTDTGCRLFLSATSSASYTMGGATSLAPQSMQLQFVKFPPRESPTRIRTLNGQIIDSQLDKTSRALDPSALGFRFSPGYFFDVVRKHPNQDMLFVSAPDTGRIKVTQPASALKYFEQGTWIELENGNRTIEIGLTTAPFAAAKQPLGFGNELAVQFDQVPGEFAVLTNTGVHR",
    "response#3": "WAPFHVVRHHNIPDKVFEHLNAGEVFTKLGLFAEIGYAWASIDSSLFLWDYTHPNPELIGYEEATHTITAVALVPPKPGVFVKTITHVLVVATTSEIILLGVSATPTPSGSKSLTLYSTRMSVHRGGSDVSFIVGTKDGRIFLGGESDTDIHEIFYQQEERWFSSRCGKINHSHPGWSAVVPSLAGLPFGSRQQEWLRGLYVDDTRNLLYSLSNRSTIRTYHMEGPEKLTKVIEKDKTSCLRDFAHMADSSPLFTDKTNIVALSPIPATEASKLHLMALTDTGCRLFLSATSSASYTMGGATSLAPQSMQLQFVKFPPRESPTRIRTLNGQIIDSQLDKTSRALDPSALGFRFSPGYFFDVVRKHPNQDMLFVSAPDTGRIKVTQPASALKYFEQGTWIELENGNRTIEIGLTTAPFAAAKQPLGFGNELAVQFDQVPGEFAVLTNTGVHR"
  },
  {
    "reference": "MKVCRVEEIRRLDSIAVEQYGVDHRILMENAGHAVYAVIEREIGVRDLRFLVISGTGNNGGDGFVVARKIESGGGEVRVVLVGDPSRLRSPAKENYELVVRRGIEVTVVQDDPSPLEGLLGWADVVVDALLGTGLNREVRGVHARIIDMVNSSGLTVVSVDIPSGVAGDTGRVMGTAVRADYTVTFGLPKIGNVLYPGHELCGRLFVSHISYPRTLVEADFVKVELNAPVPLPPRPRQGHKGTFGKALFVAGAYGYFGAPFLSSLAHLKAGGGYSRLATPRSAAATIASRAPEVVMVPMPETEKGALSRAAKERILELVGISDIVVVGPGLSLEPETQELVLELAEEVEKPLIIDGDGLTAISRNPSILEGRVAPTILTPHPGEMSRITGVPVSEILADPLPVLRRASRTLNSIIVLKIAHSLIGMPDGRVYVNMTGNPGMGTAGSGDVLNGTITAMHGLGLGIEEAVRMGVLAHGLAGDLAAALKGEDGMTAVDVLESLPDAVRILREEPWRIEENYFPKVVL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a high specificity for (6S)-NADPHX, K(+) binding, with minimal off-target effects.2. The protein design should be able to enhance the efficiency of nicotinamide nucleotide metabolic process.3. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration4. The protein must bind to Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "GVMAGSATYPGAAILCTGAAVAATSGMVRYAGTAAAEVVSHWPEVIATSDRESAGRVQAWVVGPGLGTDESAAEALRWALQTDLPVLVDADALTLLAAAPELAADRRAPTVLTPHAGEFARLAGGPPGADRVSATRGLADRLGATVLLKGNVTVIADPGSNTVYLNPAGQSWAATAGSGDVLSGVIGALLAAGLPAGRAAAAGAFVHARAARAAAEDPGPAPVPTSAAAL",
    "response#2": "EPRDVLAAWPRPGATDTKYTRGVLGVVAGASSYPGAAIMCTQAAVNAGAGMVRFLGDSTTRGMVLARSPEVVCSDDQPWDVHVQAWLAGPGVVGDESQAARVQAIIEAQEPAVLDAGALDAAARSVVDAALGPHKILTPHAGELSQVFEWLHGFGVCDSTVERSEIEANPVHWAGEAARLTGATVLLKGASTVIASPVGRNGDACEDGNASGRDECLCLSIGHASPWLATAGSGDTLAGILGTVVAHVAEHPDALAVLGDWARGDGRWAAAAALGAGLHAVASRADGEGPVPPSVLAENVRAVLIRAAEAPLLASQPEGTLMRRAAYGLAGVVAAELRRRTGGIAGRTICAVVGSGDNGGDALWAATFLRRRGVHASAVLLAPDRTHRRALAAFRKAGGRIDQTIPATTDLVIDGVVGISGRGALRPDAAAVFAATGAPVVAVDLPSGIDVHTGAAEGAHVTAAVTVTFGGLKPVHALADCGRVELIDIGLDLPTSAA",
    "response#3": "MLEKTDMKLPDRKVLNVHKGNFGHLCVYVGQKSGAGLLSAMAGLYFGAGLVSVMGKDFIAPLELMHTQILPENTSACVIGMGMGEVGEILYQMLETSPCVLDADVFYLQDMAKILDHYERLILTPHPKEFVSLLKICKFGEMSVEELLKNKIEILREFSAKYPHIVCLLKGANTLIAQNNKIYINTFGTPNLSKGGSGDVLAGMIGALVAQGYDLPEAAISASLAHSFAARLEKSTYGLTPLKLIENIKNIY"
  },
  {
    "reference": "MSLKNVMVAQSGGPTVAINASLAGVIRGVTASEQYETVYGSVNGILGILNNHLLNLTEIMNENPAYLDRLETTPAMYLGSCRHKLPDYLDDDSSYVFIFKQFELYNVGAFFYIGGNDSMDTVLKLSEYGRKIGSNVRIIGIPKTIDNDLCETDHTPGFGSAAKYIASSVLEIAHDTFIYAVKSVTIIEIMGRDAGWLTAAAALARNGYNSAPHLIYLPEVPFDTDDFLLDVKRMLFERNNVIIAVSEGIRDASGNYISASEKSVDTFGHSQLSGAGKTLEFLVKEKLGVKVRSIEVNVLQRCAAHLASKTDLMESVELGKKAVALSEEGVTASMVTMNRVSDDPYTIEYGYAEIKNIANEARSVPAEWINAAGNDVTQELIDYLTPLIQGESNVTYENGLPVYMDVSHLTKHQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must be able to metal ion binding.2. For general function, the protein need meet that Consistently, PPi-PFK can replace the enzymes of both the forward (ATP- PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions. The designed protein sequence is ",
    "response#1": "NVGVVLSGGQAAGGHNVICGVFDFINRVHPKSVLYGFLGGPHGVYTGEYKVLTKDIIDKYRNMGGFDMIKSGRHKIEADAQKQASLEVCKKLQLDGLVVIGGDDSNTNAAILAEYFQAYGSTTRVIGCPKTIDGDLKNYFVEISFGFDTACRSYSCSIGNLMMQTLTNRNVYQFIRLMGRSASLLTLECALRSHPNMTFVGEEVQAKKQSLKQLVAMTADLVEARAKLGKLYGVILLPEGLIEFIPEVGVLIQEINNIVAAGEFDRAKLTPASREVLDMLPSHTQQQLLLDRDPHGNVQVALIHTEQLLLELTTEELRRRGFKHPFNGRCTYLGYEGRSGFPSDFDATYCYALGNVAAGEMVHEKMQGRMAAIERIGVLTGGGDCPGLNAVIRAVVRKGTKVYGNEFLGFRDGWRGPLESATMPLGVDQVRGILPRGGTILGSSRTNPFASDDGGQRIRDNLAELGVDALIAIGGEDTLGAAAKLHGEGLPVIGVPKTIDNDLGATDYTFGFDTAVNIATEAIDRLHTTAESHHRTVVVEVMGRHAGWIALHSGLAGGANVILTPERPFDLDQVCEYIEERFRSQYSPIVVVAEGAVPAKAHEVTQTDQLDSFGHVRLGGIGQWLADSIEQRTGKEARAVVLGHIQRGGTPTAYDRVLATRFGLHAVDAVLEDGDMAGVLRHVVLGHLQRGGTPTANDVVFV",
    "response#2": "NVGVILSGGQAPGGHNVITGLFDTIKRLNPNNKLYGFILGPGGLVDHKYIEITEDYINDYRNTGGFDMIGSGRTKLEKIEQFEKGLEIVRELNIKALVIIGGDDSNTNACVLAEYYAAKKYGVQVIGCPKTIDGDLKNDQIETSFGFDTATKTYSELIGNIERDCNSSKKYWHFIKLMGRSASHIALECALQCQPNICIISEEVKAKNMTLNQIVENICNVIAYRAEQGLNFGVILIPEGLIEFIPSIGKLIEELNDLLAAHGNDYKGLDEEAQREYILAHLSELLGRK",
    "response#3": "NVGVILSGGQAPGGHNVIAGIFDGIKNMNGNSRLYGFLMGPGGLVDHDYKELTADIIDEYRNTGGFDIIGSGRTKLETKEQFDKGLEILKKLEIKALVIIGGDDSNTNACVLAEYYKAINAGVQVIGCPKTIDGDLKNEMIETSFGFDTACKVYAEVIGNIQRDCNSARKYWHFIKLMGRSASHIALECALQVQPNMCIISEEVEAKKLSLDDIITDIANVVARRAADGNNFGTVLIPEGLIEFIPAMKVLISELNDFLAHHQDEFNAVPKDDQLEYITKNLSAENAKVFASLPAGVARQLTLDRDPHGNVQVSLIETEKLLAEMVGNKLAEMKAAGKYNGKFASQVHFFGYEGRCAAPSNYDADYCYTLGFTAALLAGAGSVMVGLPGDVRIGVLTGGGDCPGLNAVIRAVVRKGVAEHGYEFVGFRNGWRGPLENLTRPLDIQAVRGILPRGGTILGSSRTNPFKIDGGVEAITNNLAELGVDALVAIGGEDTLGVAARLHDRGVPVVGVPKTIDNDLNATDYTFGFDTAVNIAMEAIDRLHTTAESHHRCLVVEVMGRHAGWIALHAGMAGGANVILLPERPFDIAQVAAYVEKRFQTQYSPIVVVAEGTTPLTGQMVLQDQQRDSFGHVRLGGIGQWLAGELEARTGNEARTVVLGHIQRGGTPTAFDRVLATRFGLHAIDAVKDGKTGVMTALKGTSVKLAPVEEVAKTEKIVPPQWVEATSRLVG"
  },
  {
    "reference": "MAFDKDAFLTALDSMTVLELNDLVKAIEEKFGVSAAAMAAPAAAGGGGAAAAAEEKTEFNVVLLEAGANKVSVIKAVREITGLGLKEAKDLVDGAPKNVKEAAPKADADAAKKKLEDAGAKVELK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors; Is thus essential for accurate translation..2. The protein should be optimized for stability and expression in ribosome where it will participate in translation. The designed protein sequence is ",
    "response#1": "TPPTASLIMKELGIQKGSGNAGSEVVGNISISQVAKIARMKKEDVLSYDLKATMKEVMGTCVPMGVTY",
    "response#2": "PPIGDLLLKLFGKDTGAHDARNEVIGNLSFAQLVEIALLKKDELKSKTLKSAVKQLLSTCKAMGVTV",
    "response#3": "TPPTSALILKELNIEKGSGESGTVVVGNLEIAQVAKIARMKKDDILSYSLKAAIKEVMGTCVPMGVTI"
  },
  {
    "reference": "MTDKNDALTLKKRFRGYFPVVIDVETAGFNAQTDALLEICAVTLSMDENGDLHPASTIHFHVEPFEGANLEKAALEFTGIYDPFSPLRGAVSEDHALKEIYKLVRKEQKAADCSRAIIVAHNAHFDHSFVMAASERCKLKRVPFHPFATFDTATLSGLAFGQTVLAKACKTAGMEFDNREAHSALYDTQKTAELFCTIVNQWKALGGWPLVNDDEDNNNDADLD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must have at least one Exonuclease domain.2. The Proton donor/acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. Mg(2+) is required for the proper folding and stability of the protein.4. A protein that contributes to DNA replication proofreading, tRNA 3'-end processing by functioning in cytosol with nucleic acid binding, exoribonuclease activity, producing 5'-phosphomonoesters, magnesium ion binding.5. The protein should be able to bind Mg(2+) ligand in a variety of conditions6. The protein must exhibit the following characteristics: Trims short 3' overhangs of a variety of RNA species, leaving a one or two nucleotide 3' overhang; Responsible for the end-turnover of tRNA: specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A); Also appears to be involved in tRNA biosynthesis.. The designed protein sequence is ",
    "response#1": "MKTYIVGGAVRDELLGVAVKDRDYVVVGATPEAMVAQGYTPVGKDFPVFLHPKTHAEYALARTERKTAPGYKGFVFHTDASVTLEEDLIRRDLTINAMAQDEDGSIVDPFGGKRDLERVQLREADDMLCFAGHTDVVPVGDLSHWSSPPFEPTIKDGVLYGRGAADMKTSIACFVAAVARLKATHPNHKGSIAFLITSDEEGPAVNGTVKVVETLEARNEKITWCLVGEPSSTHALGDVVK",
    "response#2": "MKTYIVGGAVRDELLGVAVKDRDYVVVGATPEAMVAQGYTPVGKDFPVFLHPKTHAEYALARTERKTAPGYKGFVFHTDASVTLEEDLIRRDLTINAMAQDEDGSIVDPFGGKRDLQRAKFRFAGERDHHTVAAISVGRTDELVLDLDYLEDAAAEVDMNVVMTASGRFVEVQGTGEEATFTLQEMNEMLALARFGAAISVGILPDQTCVADLDYEEDAAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNDLLFYGYAFTVAAETNTLMQDMVTAGEVDALVPERVWQELARGLMEVRPSRMFEVLRDCGALQRILPELDAYPVVVDVETAGLDANINALLEIAVATFKMDEYGWLCVDGSLNFHIKSFPGAIIQPESLAFNGIDLNSPLRAAVTEQEALHEIFEMVRQGIKTQGCSRAIIVAHNAFFDHSFLMAAVERVSFMEKNPFHPFVMFDTATLSGLVFGQTVLAKACACAGVFFDKNKAHSALYDTERTAMLFCELVNKWKAR",
    "response#3": "MKTYIVGGAVRDELLGVAVKDRDYVVVGATPEAMVAQGYTPVGKDFPVFLHPKTHAEYALARTERKTAPGYKGFVFHTDASVTLEEDLIRRDLTINAMAQDEDGSIVDPFGGKRDLQKAQVRRARKELEFTVAAETNTLMQDMVTAGEVDALVPERVWQELARGLMEVRPSRMFEVLRDCGALQRILPELDYPVVVDVETAGLDANINALLEIAVATFKMDEYGWLCVDGSLNFHIKSFPGAIIQPESLAFNGIDLNSPLRAAVTEQEALHEIFEMVRQGIKTQGCSRAIIVAHNAFFDHSFLMAAVERVSFMEKNPFHPFVMFDTATLSGLVFGQTVLAKACACAGVFFDKNKAHSALYDTERTAMLFCELVNKWKRD"
  },
  {
    "reference": "MKLKEIDRTAMQAWSPAQQHPIYLATGTSAQQLDASFSTNASLEIFELDLSDPSLDMKSCATFSSAHRYHKLIWGPHSMTAGERVSGVLIAGGENGNVILYDPAKIIAGDTEVIIAQKDKHTGPVRALDVNMFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPLEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHNNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFTSSPLRVLESHTRGILAIAWSMADSELLLSCGKDAKILCSNPNTGEVLYELPTNMQWCFDIQWCPRNPAILSAASFDGRLRIYSIMGGSTDGLRQKHVDQLSSSFGNLDPFGTGQPLPPLQLPQQTAPQSVVLPLKKPPKWIRRPVGASFSFGGKLVTFENAKPQQQPGIDQQPQHHYVYVSQVVTEKEFLARSTQLQEAVQSEGFVSYCQKKVDMAQADFERNVWSFLKVNFEEDSRAKYLELLGYRKDDLKNKITSALNLNKECCADGELGEAPTESDVPLLNKGDEGQTAEEHFLGERAKDSKQETEDLGSERKTFNISVSGDVDGLITQALLTGNFESAVDLCLHDNRMADAIILAIAGGQELLSRTQEKYFSKIQSKITRLITAVVTKNWKEIVLSCDLQNWREALAAVLTYARPDEFAALCDLLGNRLESEGDSLLRTQACLCYICAGNVEKLVACWSKVQDGNSPLSLQDLIEKVVILRKAVQLTQAVDPNAVGALLAEKMSQYANLLAAQGS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER); The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules .2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.3. A protein that can perform structural molecule activity in endoplasmic reticulum membrane, COPII vesicle coat, endoplasmic reticulum exit site to support intracellular protein transport, endoplasmic reticulum organization, COPII-coated vesicle cargo loading. The designed protein sequence is ",
    "response#1": "AFGGTLEIKTSRELKISGAIGPCVSLNAKGPCVSENEIGTGGTCQWKICGINPFTTLAVYFEVVNQHNAPIPQGGRGAIQFVTQYQHSSGQRRIRVTTIARNWR",
    "response#2": "QMPLIFLYVVDTCMEDEDLQALKESMQMSLSLLPPTALVGLITFGRMVHVHELGCEGISKSYVFRGNKDLTGKQIQEMLSLTKSPAAQQGRGPQVQQPPPSNRFLQPVQNIDMNLTDLLGELQRDPWPVPQGKRPLRSSGAALSIAVGLLECTFPNTGARIMMFIGGPATQGPGMVVGDELKTPIRSWHDIEKDNAKYVKKATKHYEALAHRAAASGHVIDIYACALDQTGLLEMKCCPNNTGGYMVMGDSFNTSLFKQTFQRVI",
    "response#3": "AFGGTLEIKTSRELKISGAIGPCVSLNAKGPCVSENEIGTGGTCQWKICGINPFTTLAVYFEVVNQHNAPIPQGGRGAIQFVTQYQHSSGQRRIRVTTIARNWC"
  },
  {
    "reference": "MKTLKTKFIFVTGGVCSSLGKGISIASLGLLLEGRGYKISIIKMDPYINIDPGTMSPYQHGEVYVTEDGAETDLDLGYYERFTNAVYTRKNSVTTGQIYYTVIQRERRGDYLGRTVQVIPHITDEIKSRIYHVATNNDLDFVMVEIGGTVGDIESIPFLEAIRQIRQDLGIGRVLNVHVTLVPTIAVAGELKTKPTQHSVKELMELGIIPDILLCRSSQQLSPELKSKIALFCNVTERNVIPALDIKGSIYEIPFMFHDNDYDTIVLEHFNMEKKRLAISAWDKFMKFLLNPEKGVRIAVVGKYISLQDSYRSIYEAILHGAVANRARLEIVRVDSEDIEKNPDGIGDLFKDIDGILVPGGFGDRGIEGKILAVSYARRNLIPFFGICLGLHCAVIEFARNVCGRANANSREFDLETKDPVISLLEDQEIVLEKGGTMRLGAYRCDLQKGSRVREIYGKDSVMERHRHRYEFTNRYRELFQANGMFFGGIHSASNLIETIEIPDHPWFIATQFHPEFKSKPIEPHPLFKDFIRASIQNRKN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for CTP, ATP, L-glutamine, Mg(2+), UTP binding.2. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates..3. The Nucleophile, active site, Nucleophile; for glutamine hydrolysis of the protein must be engineered to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).4. The protein should be optimized for activity in CTP biosynthesis via de novo pathway, Pyrimidine metabolism, CTP from UDP: step 2/2 pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. The designed protein must have at least one Glutamine amidotransferase, CTP synthase N-terminal domain.6. The protein design must be able to perform glutaminase activity, ATP binding, CTP synthase activity, metal ion binding in order to contribute to 'de novo' CTP biosynthetic process, glutamine metabolic process. The designed protein sequence is ",
    "response#1": "KHVFVTGGVVSGLGKGITAASLGRLLKMRGYKVASQKLDPYMNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLNKYSNLTSGKVYWNVLNRERKGEYLGQTVQVIPHITEEIKRFIYMGAESSDADVLITEIGGTTGDIESQPFLEAIRQVSLERGRENCCFIHVTLVPWLSGSNEHKSKPTQHSVKELQSLGIAPDIIVCRVDRPLEQSIKDKISLFCNVPKDCVIENDTMDCLYKAPLMLHEHGLDTVVCRRLQLPDSYISVIEAIKHAAWSSNRKPIIEWIDSKDFEKEEENLSILDGMDGIIVPGGFGKTGIEGKIAVVRYVREKKIPFLGLCLGLQLAVIEFARDVCGLKDANSSEFNPDTPHPVIDLLPEQKEVLKKSRYGATMRLGGQLVKIKPGTLAHRLYKKTEVIERFRHRYEINPKYVRLLESYGFVFSGMTPDRKIMQIGELPSHPFFIGSQFHPEFTSRALKPNPLFKGLIDSYLSVLESLKIACYHQNRELDLLWIDAEKLEKKNKKAWDELKSCAGIVVPGGFGTRGTEGKILAAQYARENKVPYFGLCLGMQMMSIEFARYLLKDKTLTSEEFDEDRKLDPSKYVIHFLPGQHKDKEKGGTLRLGAYPCDLTPGTKTEKLYGKKQVLERHRHRYEFNNIFREKLEKNGLIVSGYYQKGNLVEIVEIKNHPFMIGTQFHPEFLSRPNRPHPLFKGLINYANKNR",
    "response#2": "KYIFVTGGVVSGLGKGITAASLGRLLKARGLKVAAQKLDPYINVDPGTMSPYQHGEVFVTNDGAETDLDLGHYERFIDEDLNKYSNLTTGKVYWNVINKERSGEYLGETVQVIPHITNEIKSFVYEVGKKTDADVVITEIGGTTGDIESQPFIEAIRQISLEVGLSNCLFVHVTLIPYIQSSGEHKSKPTQHSVKELRSIGIMPNIIIARCDEPIDDSIRHKIAMFCNVKLDCVIQNITLPVLYEAPIMLDKENFSDIVCRELSLPCPEANLSEWEAMVQRFRHPSHEITIGLVGKYVELKDAYKSISEAIIHAGAKNSCKVNIEWLHAEKINTNNVKQKLSKLKGIIVAPGFGDRGIEGKITAIKYIRENNIPFFGICLGMQSAVIEYARNVLGLRNANSTEMDSKTSXPVIDLMNEQKEVTKKGGTMRLGTYDCYLKNNSNAYNAYKNNNIKERHRHRFEFNNKYKNDLEKAGLIATGINKNKNLVEIIEIAQHPWFVGVQFHPEFKSTVDNPHPLFCGFVAAAKQYSK",
    "response#3": "MTTKYIFVTGGVCSSLGKGIASASIGALLKASGFKVFPQKLDPYLNVDPGTMSPFQHGEVFVTDDGGETDLDLGHYERFMDTNCSKLSNVTTGRIYMETIKKERQGDFLGGTIQIVPHITTAIKEKIKMAAKKSKTDILMVEIGGTTGDIEGLPYLEAIRQLRHELGTKNTLFVHLTLLPYLKASKELKTKPTQSSVRELRSIGIQPDIILARADYTIDDEKISKIALFCDVEKEAVIPAYTASSIYEVPLNLEKYKIAELIGKKLGLPCPEPDLSEWKTMLRDADKSLCEALTHGGIANDTRVNIEWIEAESFEKEPHTVAERLKDKKAILVPGGYGERGTAGMISAIQYAREHQIPYMGICFGMQLAVIEVARHLAGIEKANTTEFGVTAEPVVGLLTEWLNGDVKERRSAEDDLGGTMRLGLYPAILAPGSLIQKIYGTSSIQERHRHRYELNIEYREKFEKAGLKFSGLSPDGLLPETVELEGHPWFVGVQFHPELRSRPFDPHPLFTSFIDSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASFVDSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASFV"
  },
  {
    "reference": "MDHLSLVPYEGGSAGGGGGGGKYKECMRNHAAAMGGQAFDGCGEYMPASPDSLKCAACGCHRSFHRRAAAGIGGGPVFFRPPPPPQPHSHHAALQGFLPSSVPAPAPPPQLALPYHAVPAAAWHHAAAAAAGRAGSETPPRMDDFGPGSAGGSGSGGGGIFGRKRFRTKFTPEQKERMREFAEKQGWRINRNDDGALDRFCVEIGVKRHVLKVWMHNHKNQLASSPTSAAAAAAGVMNPGAGIGLGTGLGTGISGDGDGDDDDTDDSPPRAAVSSPSPSPISV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Putative transcription factor..2. The designed protein should have DNA-binding transcription factor activity, transcription cis-regulatory region binding, metal ion binding within nucleus to regulate regulation of DNA-templated transcription.3. Incorporation of the Homeobox domain in the designed protein should not interfere with its overall stability and folding. The designed protein sequence is ",
    "response#1": "MYPGTLRSPGGSSTAGVGTSGGSGSPLPASNFTAAPVYPHYVGYPHMSNMDPHGPSLGAWSSPYSPPREDWSTYPGPPSTMGTVPMNDMTSPVFGSPDYSTLGPTSGASNGGSLPDAASESLVSLDSGTSGATSPSRSRHSPYAWMRKTVQVTGKR",
    "response#2": "PDFSGLPSFLPQTPSSRPVTYSYSSNLPPVQPVREVTFREYAIDPTASKWHHPRSNLAHCYSTAEEIMHSRDNSSSVAAEMFGKNAAGVYHHHHHPATPGNTSSNFYSAVGRNGVLPQAFDQFFETAYGTAR",
    "response#3": "PDFSGLPSFLPQTPSSRPVTYSYSSNLPPVQPVREVTFREYAIDPTASKWHHPRSNLAHCYSTAEEIMHSRDNSSSVAAEMFGKNAAGVYHHHHHPATPGNTSSNFYSAVGRNGVLPQAFDQFFETAYGTAC"
  },
  {
    "reference": "MGTLHGEKTMFASPNTFYIDESKLCFNKTHFNREDFNVERFMNLARQKSDLKTIQQDLRLYLKSVQNSMIELINDDYADFVHLSSNLVSLQDSLNKIEQDINRIWDEFKESTRESVGMAERIEQKCDELCSNREKQIEIRDRIYFLVAIEKLSEMLLHPPRKCSALWLQKAASFASELKGSTFPHSEEENAAEKIILSQLEAVLCAEGVRSAAGDCQNLPLIYSILSLTESTHSLTALLVSDLLYAEFVNEKHDESNQLKLLKQVFESVKKMRETWAEKMGTEHFRGNIRRFLDETLLTFILTFIDKCMGAVAVPSDTRLFHECFLLTQEFIDNWPSSHTCRAMLKSIRDKFNLLVYFKLETHRFGKQCDQLMSPEMFAEPETSENRENTPQLHCGVSRAIITAIEHVWSDDVYLPPIVDKLWDFTLKLLLKHFSWSQTMKNYFMEEKRDWTSMVTLRSDTGNLHQLVFDFALESIWGKFHDITVDTAPFGQCLTKHGRSIDSLCVQIDDSIIEMFSEVLHQEIAQVSDVPKQYRWTKKSPPTTHSKYVVTAIEMVENLKEKLCCEEHPHTDEIVRKVNLSAFNYFVGKGNEVLDSVEATGSSLSRFKRKTTTDSGSTVTDDDKIKQQIYHDAKYFLSYAENLVFSQADLTGLQEVVNRFDKDARSAIVQEKNQNEEAGNA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Required for normal Golgi morphology and function.. The designed protein sequence is ",
    "response#1": "LEDATEKTRVLIAKRFDEAVKADDLASVERFFKIFPLVGCHRTGIEKFSLYICQKLANKAQKELRNAQDIAKAESRLQLAYADRLTAILENFARVVEVNQPIIEAFYGQASSSLIDMVSILQHECDTEVKNLLMEFNKNRQIQYRSKQVNESTQRSAGGGNLGSNSIQALGHYRKPSGGSVDKLNPKEIDAIIAEITVMHARVELYFRFMRRRLQVHVETCVPEKEQFDIMERYEKIMKNSDLRRQMQEILSTYLLLERYFMEESVLKAIGLDTYESGQQCSSMVDDVFFILRKSIRRALTTQSINGTCAVINNVAACLDGDFVNALKAPLF",
    "response#2": "RFFKIFPLVGCHRTGIEKFSLYICQKLANKAQKELRNAQDIAKAESRLQLAYADRLTAILENFARVVEVNQPIIEAFYGQASSSLIDMVSILQHECDTEVKNLLMEFNKNRQIQYRSKQVNESTQRSAGGGNLGSNSIQALGHYRKPSGGSVDKLNPKEIDAIIAEITVMHARVELYFRFMRRRLQVHVETCVPEKEQFDIMERYEKIMKNSDLRRQMQEILSTYLLLERYFMEESVLKAIGLDTYESGQQCSSMVDDVFFILRKSIRRALTTQSINGTCAVINNVAACLDGDFVNALKG",
    "response#3": "LEDATEKTRVLIAKRFDEAVKADDLASVERFFKIFPLVGCHRTGIEKFSLYICQKLANKAQKELRNAQDIAKAESRLQLAYADRLTAILENFARVVEVNQPIIEAFYGQASSSLIDMVSILQHECDTEVKNLLMEFNKNRQIQYRSKQVNESTQRSAGGGNLGSNSIQALGHYRKPSGGSVDKLNPKEIDAIIAEITVMHARVELYFRFMRRRLQVHVETCVPEKEQFDIMERYEKIMKNSDLRRQMQEILSTYLLLERYFMEESVLKAIGLDTYESGQQCSSMVDDVFFILRKSIRRALTTQSINGTCAVINNVAACLDGDFVNALKAPLR"
  },
  {
    "reference": "MGQKVHPYSLRVKINKDWKSKWYFDKKLYSTILHEDFLIRLEIMKFLKGIKFDISDIEIIRNNPQKVTVVIVTPRPGSVIGLKGSNLEKIGQLLTKKISKKISIKIKEVKRPELDAQIIANGIAKQVENRVSYRKVLKSALSTSMLKGAQGLKIKIAGRLGGAEIARSFEVKEGRVPLHTLRANIDYGFSEAHTTYGIIGVKVWLFKGEVLGRQTNSDAGQVINKKPFRERGETVKNFDKILNNREKINEKQTRALNKKDGLSKDEASLLNKLSSSFSKERVDSNEQNIGG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The ideal protein would be one that plays a critical role in translation through mRNA binding within ribosome, ribonucleoprotein complex.2. The protein contains novel KH type-2 domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MLPVVALVGRPNVGKSTLFNRLTNTRDALVADYPGLTRDRKYGQAKFEKRQFIVVDTGGITGDEEGIDAEMAQQSLLAIEEADVVLFLVDARAGLLPADQGIADHLRRINKQIFVVANKVDGIDGDSESAEFYSLGLGAIKQIAAAHGRGVSQLLQDALKPLESDFPDMEIIDEAPG",
    "response#2": "MLPVVALVGRPNVGKSTLFNRLTNTRDALVADYPGLTRDRKYGQAKFEKRQFIVVDTGGITGDEEGIDAEMAQQSLLAIEEADVVLFLVDARAGLLPADQGIADHLRRINKQIFVVANKVDGIDGDSESAEFYSLGLGAIKQIAAAHGRGVSQLLQDALKPLESDFPDMEIIDEAPGS",
    "response#3": "MLPVVALVGRPNVGKSTLFNRLTNTRDALVADYPGLTRDRKYGQAKFEKRQFIVVDTGGITGDEEGIDAEMAQQSLLAIEEADVVLFLVDARAGLLPADQGIADHLRRINKQIFVVANKVDGIDGDSESAEFYSLGLGAIKQIAAAHGRGVSQLLQDALKPLESDFPDMEIIDEAPPT"
  },
  {
    "reference": "MITSNFKTQENVSQAITLASWLAGEYSSIKYTLEDARTNPHIRIFFRPLPYDFFGGIGFYSEEIYDHDPWHPHYQFVKRVIPQGDSVRVENYALKDPDLHTGAGQDLEILATLTPDNIELRKGCAMIFKRQGDTFVGGVEPGHKCLIPRGDGSMTYLVSEVKVTQKTWTSRDTGYDVNTHEKIWGSNSGPFKFDKVQDFSGEIPDIAIL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must be able to lyase activity. The designed protein sequence is ",
    "response#1": "QWVRTTPVFDKTDFEPVPGTAVNFKLELLQASGTFKARGAFSNLLALDDDQRAAGVTCVSAGNHAVGVAYAAMRLGIPAKVVMIKTASPARVALCRQYGAEVVLAENGQTAFDTVHRIESEEGRFFVHPFNGYRTVLGTATLGHEWLEQAGALDAVIVPIGGGGLMAGVSTAVKLLAPQCQVIGVEPEGADAMHRSFETGGPVKMGSMQSIADSLMAPHTEQYSYELCRRNVDRLVKVSDDELRAAMRLLFDQLKLATEPACATATAALVGGLKAELAGKRVGVLLCG",
    "response#2": "QWVRTTPVFDKTDFEPVPGTAVNFKLELLQASGTFKARGAFSNLLALDDDQRAAGVTCVSAGNHAVGVAYAAMRLGIPAKVVMIKTASPARVALCRQYGAEVVLAENGQTAFDTVHRIESEEGRFFVHPFNGYRTVLGTATLGHEWLEQAGALDAVIVPIGGGGLMAGVSTAVKLLAPQCQVIGVEPEGADAMHRSFETGGPVKMGSMQSIADSLMAPHTEQYSYELCRRNVDRLVKVSDDELRAAMRLLFDQLKLATEPACATATAALVGGLKAELAGKRVGVLLCH",
    "response#3": "QWVRTTPVFDKTDFEPVPGTAVNFKLELLQASGTFKARGAFSNLLALDDDQRAAGVTCVSAGNHAVGVAYAAMRLGIPAKVVMIKTASPARVALCRQYGAEVVLAENGQTAFDTVHRIESEEGRFFVHPFNGYRTVLGTATLGHEWLEQAGALDAVIVPIGGGGLMAGVSTAVKLLAPQCQVIGVEPEGADAMHRSFETGGPVKMGSMQSIADSLMAPHTEQYSYELCRRNVDRLVKVSDDELRAAMRLLFDQLKLATEPACATATAALVGGLKAELAGKRVGVLLCH"
  },
  {
    "reference": "MQATALSSGFNPLTKRKDHRFPRSCSQRNSLSLIQCDIKERSFGESMTITNRGLSFKTNVFEQARSVTGDCSYDETSAKARSHVVAEDKIGVLLLNLGGPETLNDVQPFLYNLFADPDIIRLPRPFQFLQGTIAKFISVVRAPKSKEGYAAIGGGSPLRKITDEQADAIKMSLQAKNIAANVYVGMRYWYPFTEEAVQQIKKDKITRLVVLPLYPQYSISTTGSSIRVLQDLFRKDPYLAGVPVAIIKSWYQRRGYVNSMADLIEKELQTFSDPKEVMIFFSAHGVPVSYVENAGDPYQKQMEECIDLIMEELKARGVLNDHKLAYQSRVGPVQWLKPYTDEVLVDLGKSGVKSLLAVPVSFVSEHIETLEEIDMEYRELALESGVENWGRVPALGLTPSFITDLADAVIESLPSAEAMSNPNAVVDSEDSESSDAFSYIVKMFFGSILAFVLLLSPKMFHAFRNL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein should be able to manipulate the activity of enzymes in the Porphyrin-containing compound metabolism, protoheme biosynthesis, protoheme from protoporphyrin-IX: step 1/1 pathway, either by activating or inhibiting their function.2. The protein should be optimized for stability and solubility when expressed in chloroplast, chloroplast membrane, plastid, chloroplast thylakoid membrane where it will be involved in response to oxidative stress, heme biosynthetic process, tetrapyrrole biosynthetic process.3. The protein should be designed to have high stability and activity towards 2 H(+) + heme b = Fe(2+) + protoporphyrin IX.4. For general function, the protein need meet that Produces heme for photosynthetic cytochromes, but does not seem to be involved in stress responses; Catalyzes the last step of heme biosynthesis by inserting ferrous iron into protoporphyrin IX to produce protoheme; May be involved in wound-induced supply of heme to defensive hemoproteins outside plastids The designed protein sequence is ",
    "response#1": "ISGLAVAHHLRSRGTDAVLLESSARLGGAVGTHALAGYLVEQGPNSFLDREPATRALAAALNLEGRIRAADPAAKRRYVYTRGRLRSVPASPPAFLASDILPLGARLRVAGELFSRRAPEGVDESLAAFGRRHLGHRATQVLLDAVQTGIYAGDVEQLSVAATFPMLVKMEREHRSLILGAIRAQKAQRQAALPAGTAPKLSGALSTFDGGLQVLIDALAASLGDAAHVGARVEGLAREDGGWRLIIEEHGRRAELSVAQVVLAAPAHATAKLLRPLDDALAALVAGIAYAPIAVVHLGFDAGTLPAPDGFGFLVPAEEQRRMLGAIHASTTFPFRAEGGRVLYSCMVGGARQPGLVEQDEDALAALAREELKALAGVTARPSFTRVFRWPLGIPQYNLGHLERVAAIDAALQRLPGLHLIGNAYKGVGLNDCIRNAAQLADALA",
    "response#2": "ISGLCIAQALSTKYSNLSTNFIVTEAKDRVGGNITTMEADGYLWEEGPNSFQPSDAVLTMAVDSGLKEELVLGDPNSPRFVLWNGKLRPVPSKLTDLPFFDLMSFPGKIRAGLGALGLRPSPPAHEESVEQFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGRVWVLEQKGGSIIGGTLKTIQERKDNPKPPRDPRLPKPKGQTVGSFRKGLSMLPTAISERLGNKVKVSWTLSGIAKSSNGEYNLTYETPDGLVSVRTKSVVMTVPSYVASSLLRPLSDVAAESLSKFHYPPVAAVSLSYPKEAIRSECLIDGELKGFGQLHSRSQGVETLGTIYSSSLFPGRAPPGRTLILNYIGGDTNPGILDKTKDELAEAVDRDLRRILINPNAKAPRVLGVRVWPQAIPQFLIGHFDLLDAAKAALTDGGHKGLFLGGNYVSGVALGRCIEGAYESAAEQL",
    "response#3": "VGVLLLNLGGPERLEDVGPFLYNLFADPEIIRLPFPWLQKPLAWLISSLRTRKSQENYKQIGGGSPLRRITEEQATALRQSLSDRGQAAQVYIGMRYWHPFTEEAIAQIKADGIDRLVILPLYPQFSISTSGSSFRLLQRLRDRDPEFQKIDCSVVPSWYAAPDDHEVQAMAELIAQELDKLEQPEQGHVFFSAHGVPVSYVEEAGDPYQREIEDCTRKIMETLGRSNPWTLAYQSRVGPVEWLQPYTEDALEELAERGVKDLVVVPISFVSEHIETLEEIDIEYREIAEEAGIERFLRVPALHNDRDFMQALARWVRVENMWYDADIDAVMERTKSRPIPAG"
  },
  {
    "reference": "MNSGLPASAAPLGGVGIPCVKVKFCRYYAKDKTCFYGDECQFLHDDPSMASLSLHGGGAATGGGSPVSLSLAGGAVTAAGYALGGGVGSGAPANVPKKADSLGPAVAAMEGQLLSIPGMEGALNDAGLTNSYFSSTFIGVNGFGGPPESKYSMMQTVDMHMFMGQRGKRVLGIKSSVILLSLLNKAPNPTASEFVPKGPPRMASMSQTVPAFASPIFPHPGLSSSTAAALAPGMSLSAGSSPLHSPKITPHTSPAPRRRSHTPNPNPANYMVPTNASDQGGGAHIIQKETVGGTTYFYTDNTPAPLAGMVFPTYHMYPQTAPHVAYMQPKANAPSFFMADELRQELINRHLMTMAQIDQSENPGFRLVNTKCMMLVDMWKKIQHSNTVTLREVFTTKVFGDHSLVFSYDFHAGAETMFSRHFNDAAADSYFTKRKWGQHELPPPRQHAGLLPESLIWAYIVQLSSALRTIHTAGLACRVMDPSKILITGKTRLRVNCVGMFDVLTFDNSQTNHLALMPQYQQADLISLGKVVLSLACNSLAGIQRENLQKAMELVSINYSSDLKNLILYLLSEQSRMRSVNDIMPMIGARFYTQLDASQMRNDVIEEDLAKEVQNGRLFRLLAKLGTINERPEFQKDPTWSETGDRYLLKLFRDHLFHQVTETGTPWIDLSHIVSCLNKLDAGVPEKISLVSRDEKSVLVVTYSDLKRCFESTFQELLGATNGPL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover; PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.; PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex; PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP)2. The protein should exhibit Polar residues compositional bias.3. A protein that can perform metal ion binding in PAN complex to support mRNA processing, positive regulation of cytoplasmic mRNA processing body assembly, nuclear-transcribed mRNA poly(A) tail shortening, protein phosphorylation.4. The protein must contain a signal peptide for proper functionality.5. The protein should have a high affinity for ATP.6. The designed protein should contain one or more C3H1-type, Protein kinase domains that are essential for its function. The designed protein sequence is ",
    "response#1": "PPPNRMQKTGNFSYITSCYKAVNSKDDLPYCLRRIQGFRLVNTKCMMLVDMWKKIQHSNSVTLREVFTTKAFGDHSLVFSYDFHAGAETMYSRHFNDPAADSYFTKRKWGQHEPPPPRQHAGLLPESLIWAYIVQLSSALRTIHTAGLACRVMDPSKILITGKTRLRVNCVGVFDVLTFDNSQTNHLALMPQYQQADLVSLGKVVLALACNSLAGIQRENLQKAMELVSLNYSSDLKNLILYLLTDQSRLRSVNDIMPMIGARFSGLEATLPNSYCNCMIQVLYHIEPLRVLLLSHLCTKEFCLSCELGFLFHMMSTSQGQPCQPGNFLRAFRTVPQASALGLIVSDLNPEAKAKVDLAGLIQSWNTFILHQMHSALLETKKKSEGKSMGCSSEADFGDSKIGELHSSLDERENPEESDISSLFGFKQLQIHCCLKCGKEVKKESMLLNCTLAYPTGDLEKDSWSFCDILTGSLCPQQMTRAWCEQCNKYTPNSQTGKLQSLPKILSINTALQNSQNKLFWQTQMEKMVSKASIVESTVATNMSTILSSKPCRYGDNCSRPGCRFRHSL",
    "response#2": "MESEYVQTHCVLADGGDQFGVCSLAFDKHEELLWMGNQGGHVTSYYSGGLQKYTSFQVHGSEDIRQILSVDDGVLILSPGMLRFQIRRGLPIFTHRSSNLQEMQCMLQLSPSRLLMGGHQDKLIDFNISHCKETTLIDVRQSGCALLRSHSRFVCTGDPVGLIDLRDPTTLKVEHTLETHSGSLSDFDVQGNLLVTCGFSNRQGGLAVDRFLMVYDLRMLRAVSPIQTVLDPLYLRFLPSISSRLAVVSPLGQVQLVDTVALSETRLCLLQMENPGAMCLAFDVSATSQAMALGDNTGSIHLYHRTACRTEGQIIEVFPNYHIYPPTAPHVAYMQPKANAPSFFMADELRQELINRHLITMAQIDQADMPGFRLVNTKCMVLVDMWKKIQHSNIVTLREVFTTKAFAEPSLVFAYDFHAGGETMMSRHFNDPNADAYFTKRKWGQHDGPLPRQHAGLLPESLIWAYIVQLSSALRTIHTAGLACRVMDPTKILITGKTRLRVNCVGVFDVLTFDNSQNNNPLALMAQYQQADLISLGKVVLALACNSLAGIQRENLQKAMELVTINYSSDLKNLILYLLTDQNRMRSVNDIMPMIGARFSRENQGKMFGYITSVYKAISTLDGLPYAIRRVEGFRLSSEYALQAAETWRNIQHPNIVSLKEIFVSKEFGDNSSLFFTYEFFPGSETLESKYLSQSGNPLSESVLWSFICQITSALKTIHSAGLVCRVIHPSKILLTGKNRIRMNGVGIFDVVNFDTPRILAQYQHEDLLLFGRLILTLACRSAQSTTTTNLSKSIEYVSNQYSKELYNLIVYLLTKPVINLPNIDEVVLMISGRLLQENNYLHTY",
    "response#3": "MESEYVQTHCVLADGGDQFGVCSLAFDKHEELLWMGNQGGHVTSYYSGGLQKYTSFQVHGSEDIRQILSVDDGVLILSPGMLRFQIRRGLPIFTHRSSNLQEMQCMLQLSPSRLLMGGHQDKLIDFNISHCKETTLIDVRQSGCALLRSHSRFVCTGDPVGLIDLRDPTTLKVEHTLETHSGSLSDFDVQGNLLVTCGFSNRQGGLAVDRFLMVYDLRMLRAVSPIQTVLDPLYLRFLPSISSRLAVVSPLGQVQLVDTVALSETRLCLLQMENPGAMCLAFDVSATSQAMALGDNTGSIHLPPPNRMQKTGNFSYITSCYKAVNSKDDLPYCLRRIQGFRLVNTKCMMLVDMWKKIQHSNSVTLREVFTTKAFGDHSLVFSYDFHAGAETMYSRHFNDPAADSYFTKRKWGQHEPPPPRQHAGLLPESLIWAYIVQLSSALRTIHTAGLACRVMDPSKILITGKTRLRVNCVGVFDVLTFDNSQTNHLALMPQYQQADLVSLGKVVLALACNSLAGIQRENLQKAMELVSLNYSSDLKNLILYLLTDQSRLRSVNDIMPMIGARFGGLTKPNTFGYVMSVYKATNVKNGIHYCLRRVHGFRLSSAKCMAVVDVWKKLQHSNLVHLREVFTTKAFGDHSIVFVYDYHAGAETLMNQFFGQTSAATVNGLCPTVFPATPNGITQHKAGLSTLRQHAGLLPESLIWAFVVQLSSVLRTIHAAGLACRSFEPTKILVTGKSRLRLNCCGIADVLTYDSALPNAKALVPHYQQEDLVSLGKLVLALACNTMAAIQRDNVQQSMELVARNYSADLRNLILYLLSSRTRSINDIMPMIGARF"
  },
  {
    "reference": "MLADKTILLIIGGGIAAYKALELIRRLQDQGAKVTPVLTRAGEEFVTPLSVSALAGQAVHRDLFDLTAEAEMGHIQLSRSADLVVVAPATADLMAKMANGIANDLASTLLLATDTPVLIAPAMNVRMWEHPATQRNLKTLRDDGVQVVGPDEGNMACGEFGPGRLTEPEAILAAIQSKLSDGPLRGKRVVITSGPTHEPIDPVRYIANRSSGAQGTAMARALRDLGADVVFITGPATVRPPEGVEVVPVESALEMESAVQAALPADVAIFAAAVADWRVSSASDRKLKKSKDGLPSLTFAENPDILRSVAQMKTGRPPLVVGFAAETNDVVDNATAKRLRKGCDWIVANDVSPETGIMGGQENAIVLISEHGAEPWPRLAKDEVAERLATRIAETVNSNS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that can modulate the overall flux of metabolites through the CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed.2. The designed protein should be optimized for metal ion binding, phosphopantothenate--cysteine ligase activity activity in order to improve pantothenate catabolic process.3. The protein must bind to Mg(2+) in order to perform its enzymatic function.4. The designed protein have Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains with specific sequence motifs that are crucial for its activity.5. The designed protein must have a Proton donor that is structurally stable and resistant to denaturation.6. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine. The designed protein sequence is ",
    "response#1": "MRIVLGVAGGIAAYKACELLRLLTEAGHTVRVVPTAAALQFVGAPTWAALSHQPVSADPFHDVHEVPHVRIGREADLLIVAPATADLMAKAAHGLADDLLTNTLLTVGCPVMFAPAMHTEMWEHPATVANVATLRARGVVVVEPAVERVAARIGPAARIIRDGKNVVVTAGATREKIDDVRFISNYSSGKMGFALAASAAKLGAKVTLITGKTALQTPQGVQRIDVESAREMLVAAEAFFTACDMFIAAAAVADYRPASPHEGKIKKSENVFEIQLVKNPDILQLFGTQKSSKQVAIGFALEAQNPMENAKEKLAKKNLDLIVLNSASEPGAGFEVDTNVVTLLTPGQPQVLVMVSGSVAAIKAPATLRRLREQGVQTGVIATQAALHFVTELSLSTAGNCRVATDQTWFAPQPQAQHLTLARCDAAVIVGAGADALARAAQGRAEDLLSATLLSVRAPVLWVPAMNEAMWQHPAVQRNVAQLRADGHHFLGPVSGAFGTAGEGAGMGRMAEPEDIAAQVVEAVLALVGDRRGPMAGRHVLVTAGGTREHLDPVRYLGNISSGKQGYAFARTAVARGARVTLISANVSLPDPAGVEVVRVGSTAELRTAVLEHGDDADVVVMAAAPADFRPTKYAEQKIKKAVDGTAPVIELTTNPDIAAELGAQRRPGRILVAFAAETQDFVRNAQQKLTRKNADLIVVNEVGNGKAFGTDDNAVTVVGKRVVISAGGTQENIDPVRYIGNRSSGRQGFAIAELAAQRGAEVTLVAASTDSLPVPCGTTVVRVGSTREMEAAMAEYGAGADVIVMSAAVADFRPDSQADSKLKKGADEDALSTIHLVENPDILKGLVNRRDRGELKDGAIIVGFAAETGDAAGSALDYALSKFERKGCDILMANEVGEGKVFGQKTNEGW",
    "response#2": "RIVLGITGGIAAYKAADLVRRLKEAGADVQVVMTAAAQRFVGAQTFQALSGRPVRDSLWDAAAEAAMGHIELARWPDLVLIAPASADTIARLAQGRADDLLTTLCLATDRPLAIAPAMNRLMWAHPATQQNLQVLQARGVQVLGPGSGSQACGETGDGRMWDGRRVLITAGGTQENIDPVRYIGNRSSGRQGFALAEAAAQRGAQVTIVAGNTVALPTPAGAEIVPVVSTEEMYRAVQERAAASDLIIMAAAVSDFRPATQATSKMKKGSDDGALSRVELVENPDILRWSVTARDEGEIPADTVIVGFAAETGGDGHSPLELAQQKIQRKGCDLLMCNDVSGGGVFGQARNAGWLMGLDGGPTTLPVGPKEALADVVWDLVLERLRTS",
    "response#3": "MRIVLGVAGGIAAYKACELLRLLTEAGHTVRVVPTAAALQFVGAPTWAALSHQPVSADPFHDVHEVPHVRIGREADLLIVAPATADLMAKAAHGLADDLLTNTLLTVGCPVMFAPAMHTEMWEHPATVANVATLRARGVVVVEPAVERLRDASANGTFVAYRLLLGVSGGIAAYKALETTRLAVRHGHAVRVIQTEASIRFVGTASFEAITGAQVLTSEFEPDPLRGSYPGEPLPERSPISHLALVERADVYLIAPASANTIAKLTGGHADNLLTAAALAAPCPVAVAPAMNERMYVHPATQANLEALKARGVTIVPPGEWAGLRVLVTAGGTREPIDSVRYMGNRSSGRMGFALASEAAMRGAAVTLVAANTGLAPPPGTRVIEVGTAAELAQACDEEFDVCDVLLMAAAVADFRPASPAHDKLKKEAGVPAPIELEPTEDVLSGLGARKRSDQILVGFAAEHGEGAVSYGLEKLERKHLDVLVVNDISKPGVGFDSDENEVTILWRDGEAEPIPYGPKEALADVVWDRVAALL"
  },
  {
    "reference": "MTTPRTAPDIRDESHARALDAADPLRAFRERFHIPTLGHARPVAYMTGNSLGLQPRTTRAIVEQELKDWAELAVEAHFQAAHPWYAYHEMFRDPGDRLAGAKPGACEVVMMNSLTVNLHLLMASFYRPTRERFRIVIERGAFPSDEHAVASQARFHGHDPKDAVLRLTPRKGERTLRTEDIEATLEREGHTIALLLLGGVNYATGQRFDIPRITRAAHAQGVVAGYDLAHAAGNVPLRLHEWNADFAAWCSYKYLNAGPGSLAGCFIHERHHNAALPRLEGWWGTNTTTRFLMRPDFDPGRGAEAWQISNPPVLAAAPLKASLDIFDEAGADALRTKSLALTGYLRALLEQIEGLDVVTPRDDDAHGCQLSIRLTDPGADGRALQKALLARGVVADYREPDTLRLAPVPLYNTYHDAQRAADALRLLTA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be able to bind pyridoxal 5'-phosphate ligand in a variety of conditions2. The protein must be engineered to have a cooperative binding mechanism with pyridoxal 5'-phosphate to ensure proper regulation.3. A protein with pyridoxal phosphate binding, kynureninase activity is needed to enhance tryptophan catabolic process, anthranilate metabolic process, L-kynurenine catabolic process in a specific context.4. The designed protein must have at least one Aminotransferase class V domain. The designed protein sequence is ",
    "response#1": "PRTARAAAEQQFRNWEQLAVEGHFKGETPWMHFHKRLEEASARVVGAKPVEVVVMNNLTTNLHLLLISFYQPTATRYKVLMEGGAFPSDQYALETQVRMRGLDPDDAIVELVPRPGEHTLRTEDILAKIAELGDSLCTILMGGINYYTGQVYDMAAITEAGHAVGAMVGFDLAHAAGNIPLHLHDWDVDFACWCSYKYLNSGPGGVSGAFVHERFR",
    "response#2": "AKKLMARMVGAQEHEIAILNGLTVNLHLLLATFYRPDSNRYKLMIEDHAFPSDTYVAKSQLRSHGISPEKGLILLKPRKGENLLNEDDIIRLFDEQGDQIKVALLPAVQYYTGQLLNVKRLTEAAHKKGILVGVDLAHAVGNVPIYLDEWNVDFAAWCTYKYMNAGAGSMGAIYVNEKYFTNYSETFPMYQGWWGNNVETKFLMKGDFDPALGADMFKLSNPSPVLNAMLMTSLEIFEQTSMEELRQKEYLLTGYLA",
    "response#3": "PRTARAAAEQQFRNWEQLAVEGHFKGETPWMHFHKRLEEASARVVGAKPVEVVVMNNLTTNLHLLLISFYQPTATRYKVLMEGGAFPSDQYALETQVRMRGLDPDDAIVELVPRPGEHTLRTEDILAKIAELGDSLCTILMGGINYYTGQVYDMAAITEAGHAVGAMVGFDLAHAAGNIPLHLHDWDVDFACWCSYKYLNSGPGGVSGAFVHERFH"
  },
  {
    "reference": "MASRVSSKPVALILLAAGQGSRMNSDLPKVLHQIGSAPLAVHALRAATALEPEKTVVVTGHGADKVEATLSKHYDDLIFARQTEQNGTAHAVDQARSALEGFAGDAVVLYGDTPFVSGDTLQHMADARKTADVVVLGFKADKPGRYGRLITNGASLEKIVEAKDATAEQLAVKLCNSGVIMCQSQTLFELISEVQNNNATGEFYLTDIVGLARERGLTAVAVTCDEAETLGINTREELAAAERTFQTRMRAEAMETGVSLADPDSTFFAYDTVLGRDAMIGPNVVFGPDVTVESGAEIKAFCHLEGCHVSQGSVVGPFARLRPGAELEEGAKVGNFVEIKNATLAEGAKVNHLSYVGDAHVGAGANIGAGTITCNYDGVFKHRTTIGAGAFIGSNTLLIAPVDVGADAMTGSGTVVTEDVPDGDLALSRVRQTNKAGFARKLMQMLRAKKMKKDA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The acetyl-CoA, Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.2. The designed protein should contain one or more MobA-like NTP transferase domains that are essential for its function.3. The protein design should consider the endogenous proteins and enzymes present in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway to avoid interference with native metabolic processes.4. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.5. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.6. The ideal protein would be one that plays a critical role in UDP-N-acetylglucosamine biosynthetic process, peptidoglycan biosynthetic process, lipid A biosynthetic process, cell morphogenesis through UDP-N-acetylglucosamine diphosphorylase activity within cytoplasm. The designed protein sequence is ",
    "response#1": "VVILAAGKGTRMYSDLPKVLHLLAGKPMVQHVIDAAQGVGADKVHLVYGHGGERLQSTLQDPSLNWVLQAEQLGTGHAMQQAAPCFADDEDILMLYGDVPLISTETLQRLRQAKPGQLGVLTVTMDDPTGYGRIVRENGAVTGIVEHKDATPAQREIREVNTGILVANGADLKRWVARLDNRNAQGEYYITDIIAMAHQEGRSIRAVHPERLHEVEGVNNRLQLARLERAHQADIAEQLLLASAMEDGVTLLAPETVFFAHDTVIGRDTVLEQNIVFGPGVTVESGARIRAFSHLEGCHVSQRATVGPYARLRTGAELAEDVHVGNFVEIKNAYLDEGAKANHLTYIGDASVGKATNIGAGTITCNYDGVMKHHTEIGANVFIGSNTMLVAPVTVGARAMTASGSVITSDVPPEALAMGRAKPPVKISGWRRPVKE",
    "response#2": "MTLAVILAAGEAKRMRSRQPKVLHPLCGRPLIAYALRTARALADRTVVVTGPNADDVRVAAGDGATFVEQRERLGTGHALGQARAACGDGPIVVLAADMPLLSSETLERLVAHHRGAGAAATVLTAVVDRPQGYGRILRQGGRVKRIVEDRDATDDQKKITEINTSVYCFEARRLWKALAEGEDGEYYITDLAALGVMLKDPARFDLRGELKHGRDVEIDTNVIIEGKVQLGNRVKIGSGCVLKNCMIGDDCEISPYSVLENAELAADCTVGPFARLRPGSSLAAGAHVGNFVEMKKATLGKGSKAGHLSYLGDASIGANVNIGAGTITCNYDGVNKSLTVIGDDVFVGSDTQLVAPVSVAAGTTIAAGTTVMKDISAPGLAYNRKEGVTMIDPNRVDVRGKLTCGRDVVLDINTIFSGDVVLGDDVQIGANSIISNASIGAGTRILPNCIIDQTTVGKSCEIGPFARTRPGVELDDQAKLGNFVEIKKSRIGKGSKVNHLTYIGDTTIGERVNVGAGTITCNYDGVNKHPTVIGDGAFIGSGVELVAPVNIGAGATIGAGATVSKDAPAGELTVERARQRNIEGWVARKRRLAKERR",
    "response#3": "VLILAAGQGTRMKSDTPKVLHEIAGKPMLAHVIETAQQLNPAKIVVIYGHGGEQVRQQLSSYEVEWALQEQQLGTGHAVVQGMPALDSVDKVLVLYGDVPLIQADTLQQLIDASMETSIGMLTVVLDNPTGYGRIVRNAAGRVVRIVEEKDASPAERAIREVNTGILAAPAVHLRRWLARLSNDNAQQEYLLTDIVAMAVADGVPVVSAQPAGVWEVAGVNSKVQLAELERRHQMARAARLMEQGVTIVDPETTWIDSDVSIGRDATILPGTQLLGATSVGADARIGPDTTLTDTEVGDGAEVKRTEANLAQIGAGATVGPYSYLRPGTVLGAKGKIGGFVETKNARIGDGAKVPHLTYCGDATIGEGANIGAGTIFANYDGVAKHHTTIGAHSFVGSDSVLIAPVDVADGAYVAAGSALTGDVEPGQIAVARGRKPVTLRGWKRPVKKPAGG"
  },
  {
    "reference": "MSEEKPKIVFVLGGPGAGKGTQCAKIVDRFGFVHLSAGDLLREERAREGSEVGALIEDYIRNGKIVPVEVTCSLLEAAMNKSDKNKFLIDGFPRNQDNLDGWNRQMSEKVDFQFVLFFNCSEDVCVDRCLNRGKSGSGRSDDNMESLKKRIQTYNNDSLPIIKHFEELGKVKQIDASPSSDEVFSKVEEVFVQSGF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be designed to catalyze the following reaction ATP + dCMP = ADP + dCDP.2. The protein should be able to bind CMP ligand in a variety of conditions3. A protein that can perform dCMP kinase activity, CMP kinase activity, ATP binding in cytoplasm, nucleus to support pyrimidine nucleotide biosynthetic process, phosphorylation.4. The protein must exhibit the following characteristics: Plays an important role in de novo pyrimidine nucleotide biosynthesis; Has preference for UMP and CMP as phosphate acceptors.; Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. The designed protein sequence is ",
    "response#1": "MPDVVSWNSIISGHSKRPEALVYFEQMVSGGTLPDSITFVAILSACALL",
    "response#2": "MTKKLVIATNNQGKYREFRELLAGTDYELVSLDEIGCDVDIPETGTTFVENALIKARHACRISGLPAIADDSGLEVEALGGAPGVYSARYGGDGKSDADNNAKLLAELADVPWEKRTARFRCVIVYLRHADDPVPLICQGVWEGSILREPRGNGGFGYDPIFFVPELGCTAAELPREVKNARSHRGQALARLVELLRERW",
    "response#3": "MADSGAAE"
  },
  {
    "reference": "MKYVKSSGQSRQQQKGRKPETSRKTMSRPGTTAKKGQDGGKGAAGGKGVNGKVSGRESRRATGNPIEKGEKGEKSDKSPDEEAKEEEPPEEERKFEPASHADVDLVDMLERDILQKNPNIRWDDIADLVEAKRLLEEAVVLPMWMPDYFKGIRRPWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPSTIFIDEIDSLCSRRGSESEHEASRRVKSELLVQMDGISSDEATKVVMVLAATNFPWDIDEALRRRLEKRIYIPLPNDDGREALLKINLREVQLDPSVDLKSIARKLQGYSGADITNVCRDASMMSMRRKIAGLKPEQIKLLAKEEVDLPVSTKDFIEAISKCNKSVSKHDLEKYEQWMREFGSS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein can be expressed in spindle pole, centrosome and perform microtubule severing ATPase activity, isomerase activity, microtubule binding without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "LESKINNMHVAFADKESEKNWSLREKHVTQIRKLLRGNALQDYPSQFAAAYKSVIDGVLKSATSLRTTLSNQGLLLVKESGQLGGNAFVDPVMDIVFPQIIRLTGQMKKITSNNAHITVCGLLTSATFSTKLINHVTSATVEKNAQPRTFAAVWLRILILSHSQTHKSAMQNHGGLDQIEKAIAKGLQDPTPSVKENMRVTYWSFAEYWPSEADKIYRKLDTKAKAMR",
    "response#2": "LESKINNMHVAFADKESEKNWSLREKHVTQIRKLLRGNALQDYPSQFAAAYKSVIDGVLKSATSLRTTLSNQGLLLVKESGQLGGNAFVDPVMDIVFPQIIRLTGQMKKITSNNAHITVCGLLTSATFSTKLINHVTSATVEKNAQPRTFAAVWLRILILSHSQTHKSAMQNHGGLDQIEKAIAKGLQDPTPSVKENMRVTYWSFAEYWPSEADKIYRKLDTKAKAMS",
    "response#3": "LESKINNMHVAFADKESEKNWSLREKHVTQIRKLLRGNALQDYPSQFAAAYKSVIDGVLKSATSLRTTLSNQGLLLVKESGQLGGNAFVDPVMDIVFPQIIRLTGQMKKITSNNAHITVCGLLTSATFSTKLINHVTSATVEKNAQPRTFAAVWLRILILSHSQTHKSAMQNHGGLDQIEKAIAKGLQDPTPSVKENMRVTYWSFAEYWPSEADKIYRKLDTKAKAMG"
  },
  {
    "reference": "MNKELISEIPLLEDKAVSEIENAASLKDLEKVRLSYLGRKGVVKAYFDDLKNIDDAGEKRDLGAVINVLRNKIDQLITSKENELKDKEVKLKLQNEAVDITLPVRPERIGKIHPLSKVISEVKLIFAHMGFKAVDGPDIEDEFHVFDALNTPSHHPAREEQDTFYLRNKINDKRMVLRTHTSSVQIRTMEKTKTFPIKIVAAGRVYRNDFDATHTPMFHQIEGLYVNENVNMGQLKFTIHCFLSKFFGDKGLRIRFRNSFFPFTEPSAEVDISYKDSKWIEVLGCGMVHPNVFKNVGIDHTKYSGFAFGIGIERLAMLKYQISDLRSFYDNRVSWLNHYGFHFSSLR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design must be able to perform tRNA binding in order to contribute to phenylalanyl-tRNA aminoacylation.2. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.3. The protein should be designed to have high stability and activity towards ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) +   L-phenylalanyl-tRNA(Phe). The designed protein sequence is ",
    "response#1": "IESFFGDLGFEVKDGPEVEDDYHNFDALNIPEHHPARQDHDTFYFNPKLVLRTQTSGVQIRTMEVEQPPLRIISPGKVYRNDYDQTHTPMFHQVEGLMVDKDVSFTHLKGILHDFLHHFFEEEVEIRFRPSYFPFTEPSAEVDIMGKNGKWLEVLGCGMVHPNVLRSVGIDPEVYTGFAFGMGVERLTMLRYGVNDLRAFFG",
    "response#2": "VREVMIGLGFEEVRGPVLEVEFWNFDALFQAQDHPAREVHDTFYIRWEGPIEAPPEHLLEAVGRVHEEKWRYKWSREKALNPVLRTQTTAVTIRALAERGDGEYKVFTIGRVFRPEKLDPKHSMEFHQLDGIVVGPGLTFKHLLGQLEQIAKALGMTRVRFRPAYFPFTSPSVEVYAEHPKLGWVEFGGAGIFRPEVTEPLGVRKSRVLAWGWGLDRIAMILLGIDDIRELFTKDG",
    "response#3": "IPSVVPTAPAGRGLTPEQRRRRAVSRALAFAGGVEVPPPVFLPAGVFDTWGLDADDPRRTTTRVLNPLDVERAELATTLLPGLLEVAQRNISRGARDLTLYGIAQVVLPTGDTKPVAPLPVDRRPTDEQIAELLGSLPDQPVHVAAVLTGRREPRGPWGPGRQAEAADAFALVDAVADAAGVVIERRPAAYLPWHPGRCAELVVEGRVVGYAGELHPAVLERSGLPARTCALELDL"
  },
  {
    "reference": "MGNREMEELIPLVNRLQDAFSALGQSCLLELPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLVLQLVTSKAEYAEFLHCKGKKFTDFDEVRHEIEAETDRVTGMNKGISSVPINLRVYSPHVLNLTLIDLPGITKVPVGDQPPDIEYQIRDMIMQFITRENCLILAVTPANTDLANSDALKLAKEVDPQGLRTIGVITKLDLMDEGTDARDVLENKLLPLRRGYVGVVNRSQKDIDGKKDIKAAMLAERKFFLSHPAYRHIADRMGTPHLQKVLNQQLTNHIRDTLPNFRNKLQGQLLSIEHEVEAFKNFKPEDPTRKTKALLQMVQQFAVDFEKRIEGSGDQVDTLELSGGAKINRIFHERFPFEIVKMEFNEKELRREISYAIKNIHGIRTGLFTPDMAFEAIVKKQIVKLKGPSLKSVDLVMQELINTVKKCTKRLANFPRLCEETERIVANHIREREGKTKDQVLLLIDIQVSYINTNHEDFIGFANAQQRSSQVHKKSTIGNQGTNLPPSRQIVIRKGWLTVSNIGIMKGGSKGYWFVLTAESLSWYKDDEEKEKKYMLPLDNLKVRDVEKGFMSSKHVFALFNTEQRNVYKDYRSLELACDSQEDVDSWKASLLRAGVYPDKSFTENDENGQAENFSMDPQLERQVETIRNLVDSYMSIINKCIRDLIPKTIMHLMINNVKDFINSELLAQLYSSEDQNTLMEESVEQAQRRDEMLRMYQALKEALAIIGDINTVTVSTPAPPPVDDSWLQHSRR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in presynapse that is critical for postsynaptic neurotransmitter receptor internalization.2. The protein should be designed to catalyze the following reaction GTP + H2O = GDP + H(+) + phosphate. The designed protein sequence is ",
    "response#1": "KQPIGRLLFRQFCDTKPDLKRCIEFLDAVAEYEVTIEEEQREFGLAIFSRFFKEKSEVPLPEIPPDIVKECKWNLKQNSPSQNVFEECAGIVCKYLSETPFEEYQESTYFNRFQ",
    "response#2": "RFLTCGSVDDGKSTLIGRLLYDTKLIFEDQLAALESDSARHGTTGTDIDFALLVDGLESEREQGITIDVAYRFFATAKRKFIVADTPGHEEYTRNMATGASTADLAVVLVDSRQGILPQTRRHSYIASLLGIRHIVVAINKIDLMSYSEDVYDRIVADYLEFAKDLGFETIVPIPMSARYGDNVTMRSEHMDWYKGPVLLEHLETVPVDHIRNFSIIAHIDHGKSTLADRIIQLCGGLSDREMEAQVLDSMDIEKERGITIKAQTAALSYKARDGQVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVEVVPVLNKIDLPQADPDNAIQEIEDVIGIDAQDATPCSAKTGQGVQDVIEALIAKVPPPRA",
    "response#3": "FRQYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKRILEKLHSRFVVSLAYTYETKDALCLVLTIMNGGDLKYHIYNLGDPGFEEPRAVFYAAELCCGLEDLQRKRIVYRDLKPENILLDDHGHIRISDLGLAMEVPEGEMVRGRVGTVGYMAPEIINHEKYTFSPDWWGLGCLIYEMIAGHSPFRKYKEKVNREELERRVKNETEEYSERFSEDAKSICSMLLIKDPSKRLGCQRDGVSAVKQHPIF"
  },
  {
    "reference": "MGPNIYLTGFSGSGKTTVGKQVAAMTGWSYRDTDDEIVAATGRAIEDIFREDGEAAFRKLERSVLESVSQDERQVVSTGGGIVVDERNRRTMEATGIIVCLEARADTIYRRVSGPEETHDEQAVRPLLQDSDPLRRILSLKAERQAVYALAHWTVHTDDLSITEAASEVVRARDICSNRANSRQTHDADLAATVHTSSGDYPVWVGWGLSHTVGERVKTLLDPGAAYVITDNFVHRHARTVQMSMEAAGIPSHIFVMESGERHKSLDTLLHIYRWLAERKAERRHVVVAVGGGVVGDVAGYAAATYLRGMPVVQAPTTLVAMMDAAVGGKTAVDLPQGKNLVGAFKQPEFVLADVEALSTLSERALVSGWAEAIKHALILDEPLLRVFEENVDSVASLEPETASDALRRSVAIKADVVSRDERETLGIRALLNYGHTTAHALEAVTGYERYLHGEAVSIGMMAAADISKKMGLLSDEDIVRQRSVLEAYGLPLKYRDADPAAIKDAMSMDKKTVGGRIRWVLLDGIGNAVTRSDVPPEYIDQALREVSE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The presence of NAD(+) is crucial for the protein to function optimally under specific conditions.2. The ideal protein would be one that plays a critical role in chorismate biosynthetic process, phosphorylation, aromatic amino acid family biosynthetic process through 3-dehydroquinate synthase activity within cytoplasm.3. The protein have enhanced ATP + shikimate = 3-phosphoshikimate + ADP + H(+).4. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+), Mg(2+) binding.5. The protein must exhibit the following characteristics: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.. The designed protein sequence is ",
    "response#1": "TMPAGEAYKNLETVTELYATLLAGGLDRGGLILALGGGVVGDVAGFTAATYMRGVALAQLPTTLLAMVDSSVGGKTGVDLPYGKNLVGAFKQPIMVLIDPLVLKTLPEPELSRIXFXXGVDGLAEVVKYGVIADPMLLNYLDEQAPLILRRHPTTLRAIVVRSAAIKASVVAADEREETGLRMVLNYGHTFAHALEAATHFALLHGEAVAIGMTAEAYLAWRLNLCDREVLERQTQVLRRLGLPTRLTELPGAPTVSVDGLMAAMWRDKLAEVIKAAIIADRSFAEWLSANLPGVLRRDSSSIREAVARSIAIKATVVASDPTETGERAILNYGHTVGHALERVIGFGRVRHGEAVAWGMEVAARISLMAEACTQASVTAQHALLTDAGLLAERPAASHAKLIDAMRHDKKAAXMLKGXXRGXSXWXLTIPAGEASKSQTQLNKIYDWLLEQRAERREALIALGGGVIGDMVGFAAASYLRGVPLIQIPTSLLAQVDSAIGGKTGINHPKGKNLIGAFYHPRLVLADPSTLLTLPERNXIXXPGMAEVVKSGLIASPTLLEELSGMLQNRHTNETKRSDIVDGRPMSVAKNWPKNGSILTMGDLQLLIVESILIKRDIVEVDPFESGYRKVLNLGHTFAHAIEQVSNYQVRHGQAVAIGLVAAINLSAKLGHCSPEIEGYIERLLNSLVLPIRIPAGLAPGKLIRAMGSDKAVXVLXGX",
    "response#2": "MELGMAEVVKSGLIASPTLLEELSGMLQNRHTNETKRSDIVDGRPMSVAKNWPKNGSILTMGDLQLLIVESILIKRDIVEVDPFESGYRKVLNLGHTFAHAIEQVSNYQVRHGQAVAIGLVAAINLSAKLGHCSPEIEGYIERLLNSLVLPIRIPAGLAPGKLIRAMGSDKAIIISDSEGMAEVVKSGLIASPTLLEELSGMLQNRHTNETKRSDIVDGRPMSVAKNWPKNGSILTMGDLQLLIVESILIKRDIVEVDPFESGYRKVLNLGHTFAHAIEQVSNYQVRHGQAVAIGLVAAINLSAKLGHCSPEIEGYIERLLNSLVLPIRIPAGLAPGKLIRAMGSDKAIIISDSEAGMAEVVKSGLIASPTLLEELSGMLQNRHTNETKRSDIVDGRPMSVAKNWPKNGSILTMGDLQLLIVESILIKRDIVEVDPFESGYRKVLNLGHTFAHAIEQVSNYQVRHGQAVAIGLVAAINLSAKLGHCSPEIEGYIERLLNSLVLPIRIPAGLAPGKLIRAMGSDKAIIISDSETGMAEVVKSGLIASPTLLEELSGMLQNRHTNETKRSDIVDGRPMSVAKNWPKNGSILTMGDLQLLIVESILIKRDIVEVDPFESGYRKVLNLGHTFAHAIEQVSNYQVRHGQAVAIGLVAAINLSAKLGHCSPEIEGYIERLLNSLVLPIRIPAGLAPGKLIRAMGSDKAIIISDSE",
    "response#3": "TMPAGEAYKNLETVTELYATLLAGGLDRGGLILALGGGVVGDVAGFTAATYMRGVALAQLPTTLLAMVDSSVGGKTGVDLPYGKNLVGAFKQPIMVLIDPLVLKTLPEPELSRVCAAVEGMAEVVKSGLIASPTLLEELSGMLQNRHTNETKRSDIVDGRPMSVAKNWPKNGSILTMGDLQLLIVESILIKRDIVEVDPFESGYRKVLNLGHTFAHAIEQVSNYQVRHGQAVAIGLVAAINLSAKLGHCSPEIEGYIERLLNSLVLPIRIPAGLAPGKLIRAMGSDKVGGKVVVEGMAEVVKSGLIASPTLLEELSGMLQNRHTNETKRSDIVDGRPMSVAKNWPKNGSILTMGDLQLLIVESILIKRDIVEVDPFESGYRKVLNLGHTFAHAIEQVSNYQVRHGQAVAIGLVAAINLSAKLGHCSPEIEGYIERLLNSLVLPIRIPAGLAPGKLIRAMGSDKVGGKVVVE"
  },
  {
    "reference": "MQLVFASNNKNKIKEIQLLVPSEIQILSLEDIGCNVDIPETANTIEGNAILKADYVTKNYGYSCFADDSGLEVDVLNGAPGVYSARYAGEEKDDDKNIDKLLFELSEKTNRKANFKTVIALNLNGKQHLFTGIIDGEIIYERRGTNGFGYDPVFIAEGYEKTFAELTIEEKSSISHRGKAVKQLTEFLKSK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must possess dITP diphosphatase activity, ITP diphosphatase activity, metal ion binding to support purine nucleoside triphosphate catabolic process under certain conditions.2. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..3. The protein requires Mg(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "MXATGSK",
    "response#2": "MWRR",
    "response#3": "MI"
  },
  {
    "reference": "MLSFVDNRILLLLAVTSLLASCQSGGLKGPRGAKGPRGDRGPQGPNGRDGKAGLPGIAGPPGPPGLGGNFAAQFDGGKGSDPGPGPMGLMGSRGPNGPPGAPGPQGFTGHAGEPGEPGQTGSIGARGPTGSAGKPGEDGNNGRPGKPGDRGGPGTQGARGFPGTPGLPGMKGHRGYNGLDGRKGESGTAGAKGETGAHGANGSPGPAGSRGLNGERGRAGPAGPAGARGADGSTGPAGPAGPLGAAGPPGFPGAPGPKGEIGGAGSNGPSGPQGGRGEPGINGAVGPVGPVGNPGNNGINGAKGAAGLPGVAGAPGFPGPRGGPGPQGPQGSTGARGLGGDPGPSGQKGDSGAKGEPGHSGVQGAAGPAGEEGKRGSTGEVGATGPAGLRGARGGAGTRGLPGLEGRGGPIGMPGARGATGPGGIRGAPGDAGRAGESGLTGARGLPGNSGQGGPPGKEGPPGAAGLDGRTGPPGPTGPRGQPGNIGFPGPKGPGGEAGKGGDKGPTGATGLRGGPGADGNNGAPGPAGVVGNTGEKGEQGPAGAPGFQGLPGPAGPAGEAGKAGNQGMPGDQGLPGPAGVKGERGNSGPAGSAGSQGAIGARGPAGTPGPDGGKGEPGSVGIVGAAGHQGPGGMPGERGAGGTPGPKGEKGEGGHRGLEGNMGRDGARGGPGPSGPPGPSGANGEKGESGSFGPAGPAGLRGPSGERGEGGPAGLPGFAGPPGSDGQSGPRGEKGPAGGKGDVGPAGPAGPSGQSGPSGASGPAGPP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be expressed specifically in the collagen trimer The designed protein sequence is ",
    "response#1": "DCLPTEVRNCQDLLERGETLNGWYKIYPTEEVSMLVFCDMSTDGGGWLVFQRRQDGSVNFYREWESYKKGFGRQGSEFWLGNDKIHLLTNSGTQQLRIDLADFENIHTFATYSSFSIGNETEKYKLTLGSHLDGNMGDSLTLQNGRSFSTKDRDNDVSHIHCAVNFKGAWWYRKCHESNLNGLYHKGKHATYANGINWLTGKGYHYSYKYADMKIRPQC",
    "response#2": "SVNIAFLIDGSSSVGDSNFRLMLEFVSNIAKTFEISDIGAKIAAVQFTYDQRTEFSFTDYNTKENVLAVLANIRYMSGGTATGDAIAFTVRNVFGPIRDSPNKNFLVIVTDGQSYDDVRGPAAAAHDAGITIFSVGVAWAPLDDLRDMASKPKESHAFFTREFTGLEPIVSDVL",
    "response#3": "LGSVADVIELHKKLQHLKSPTGTKDSPARSCHDLFLEDNSTSDGYYWIDPNGGCIGDAVKVFCNFTGGVQQTCISATKNAGDLKSWSGHSIWFSDMLGGFKLTYDISRSQLQFIRAASRHAVQSFTYKCRNSAAAVIFRTQDNKEIAANKVTYDGCKSRPSVPDAAFVAVETKRVEQLPIRDFASSDIAGQHQEFGFEMGPACFS"
  },
  {
    "reference": "MDDQRDLISNHEQLPILGQRARAPESNCNRGVLYTSVSVLVALLLAGQATTAYFLYQQQGRLDKLTVTSQNLQLENLRMKLPKSAKPVSPMRMATPLLMRPLSMDNMLQAPVKNVTKYGNMTQDHVMHLLTKSGPVNYPQLKGSFPENLKHLKNSMNGLDWKVFESWMKQWLLFEMSKNSLEEKQPTQTPPKVLTKCQEEVSHIPDVHPGAFRPKCDENGNYMPLQCHGSTGYCWCVFPNGTEVPHTKSRGRHNCSEPLDMEDPSSGLGVTKQDMGQMFL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should contain Extracellular topological domains.2. The protein should be optimized for Golgi apparatus, lysosome, NOS2-CD74 complex, cell surface-specific folding and stability, while maintaining cytokine binding, macrophage migration inhibitory factor binding, CD4 receptor binding, MHC class II protein binding.3. The designed protein should contain one or more Thyroglobulin type-1 domains that are essential for its function.4. The protein should have Helical; Signal-anchor for type II membrane protein domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "MLLLPLLLLAVIVPGGDNEDVFQGPTSFHVIQISTFANSTWAQNQGSGWLDNLQLYGWDSDPGTTIFLKPWSKGNFSDEEVTELEELFRVYLIGFTLEVQDHVSEFQLEYPFVIQDIAGCELHPGKAVESFLKGAFGGLDFVSIKNDSCAPVPEGGSMAQRFYELIIQYHAICDTIAKLLLETCPRYFLSVLDAGKAC",
    "response#2": "MLLLPLLLLAVIVPGGDNEDVFQGPTSFHVIQISTFANSTWAQNQGSGWLDNLQLYGWDSDPGTTIFLKPWSKGNFSDEEVTELEELFRVYLIGFTLEVQDHVSEFQLEYPFVIQDIAGCELHPGKAVESFLKGAFGGLDFVSIKNDSCAPVPEGGSMAQRFYELIIQYHAICDTIAKLLLETCPRYFLSVLDAGKAC",
    "response#3": "MLLLPLLLLAVIVPGGDNEDVFQGPTSFHVIQISTFANSTWAQNQGSGWLDNLQLYGWDSDPGTTIFLKPWSKGNFSDEEVTELEELFRVYLIGFTLEVQDHVSEFQLEYPFVIQDIAGCELHPGKAVESFLKGAFGGLDFVSIKNDSCAPVPEGGSMAQRFYELIIQYHAICDTIAKLLLETCPRYFLSVLDAGKACS"
  },
  {
    "reference": "MTDQVRSDVQEKLVQSGEYEKLSQHIQARLRNSGWYDKVSALAQEEASKQDKVELSSLLEKVQPQACDLVDDDIKVETLKMIASFLEGALK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: May also be involved in cytoplasmic mRNA decay by interaction with components of P-bodies .; The TREX-2 complex functions in docking export- competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). The designed protein sequence is ",
    "response#1": "MCPEKERVLREFQRQVAYYELQPGSDELICHERALKQYSRSSADQETPLPHELRNETALHMTMSYLMHEIMDISERQDPQSHMGDWFHFVWDRTRSIRKEITQQELCSLGAVKLVEQCARFHIHCAARLVDADPSVFDSKINAENLTKCLQTLKYMYHDLRIKGVPCPKEAEFRGYIVLLNLADANFLWDIGQLPAELQSCPEVRQAIQFYLALQDTNFVRFFQLLADKDTSYLSACILVNYFTRLRVLGLHRLIQAYRSPRKDEVSSLPLSYIAELLSFASEQEAADFVQHYGLQII",
    "response#2": "MCPEKERVLREFQRQVAYYELQPGSDELICHERALKQYSRSSADQETPLPHELRNETALHMTMSYLMHEIMDISERQDPQSHMGDWFHFVWDRTRSIRKEITQQELCSLGAVKLVEQCARFHIHCAARLVDADPSVFDSKINAENLTKCLQTLKYMYHDLRIKGVPCPKEAEFRGYIVLLNLADANFLWDIGQLPAELQSCPEVRQAIQFYLALQDTNFVRFFQLLADKDTSYLSACILVNYFTRLRVLGLHRLIQAYRSPRKDEVSSLPLSYIAELLSFASEQEAADFVQHYGLQII",
    "response#3": "MCPEKERVLREFQRQVAYYELQPGSDELICHERALKQYSRSSADQETPLPHELRNETALHMTMSYLMHEIMDISERQDPQSHMGDWFHFVWDRTRSIRKEITQQELCSLGAVKLVEQCARFHIHCAARLVDADPSVFDSKINAENLTKCLQTLKYMYHDLRIKGVPCPKEAEFRGYIVLLNLADANFLWDIGQLPAELQSCPEVRQAIQFYLALQDTNFVRFFQLLADKDTSYLSACILVNYFTRLRVLGLHRLIQAYRSPRKDEVSSLPLSYIAELLSFASEQEAADFVQHYGLQIC"
  },
  {
    "reference": "MNRSPYRPGISAPTGAPEVSLPSLRRGDLAAAVAAFRRGEPVLVVDDENRENEGDLVAAAELMTPELMAFIVRHTGGVVCVSMDADRLAALRLPQMVQDTEDPKGTAFTVSVDLRTGISTGISAADRAATVRALADPSSTAEDFVRPGHIFPLRARPGGVLERAGHTEAAVDLSRLAGLRTAAVISEVVNDDGSMARRGDLRRFAVQHDLHLISVDDLARHRRAAEPVVRRAAQTSLPTDHGTFRAYCYTGTGDAVEHMAMVMGDVRDGRDVLVRVHSECLTGDLFGSHRCDCGQQLDAGLRLIAATGRGVLVYLRGHEGRGIGLANKMRAYELQEQGLDTLDANLALGLPVDLRRYDVAAQVLIDLGVRSVRLMTNNPDKSRGLVEHGVAVSTREPIVVVPGPANVRYLRAKRDRLGHELGALDEEVHALRGEAGLT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein with the reaction GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate, the Proton acceptor; for GTP cyclohydrolase activity, Nucleophile; for GTP cyclohydrolase activity must be optimized to ensure efficient catalysis.2. The designed protein should interact with enzymes and substrates within the 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway to enhance or regulate its activity.3. A protein with GTP binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity functions is required to enable riboflavin biosynthetic process.4. The presence of GTP cyclohydrolase II domains is critical for the interaction of the designed protein with its intended targets.5. For general function, the protein need meet that Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate. The designed protein sequence is ",
    "response#1": "VSVQMPTKYGNFELIAFKQVNTGEIHMALKKGEWEKGEPVLVRVHSSCVTGDILHSLRCDCGEQLAAAMKMVEKEGKGLILYMNQEGRGIGLMNKLKAYKLQEEGRDTVEANLELGFQMDERDYGVGAQILRHLNICKLRLITNNPRKRAGLLGYGLEVVENVVGEDRRVAEATLPTKYGGEFKIIVYENEVDDMKHIALVKGDIQPEDEVLVRVHSECVTGDLFGSLRCDCGDQLHKAMEMVGQEGKGVIVYMHQEGRGIGLANKIRAYDLQEQGRDTVEANIELGFKEDLRDYGIGAQILVDLGVRKMRMITNNPKKIVGVEGYGLTVVERVEANFPTRFGAFRLYLFKDKLSGEEHIALIKGDIKGKKNVLVRVHSQCFTGDVLGSLRCDCRDQMEKALKIINDEGQGVFLYMRQEGRGIGLSNKVHAYHLQDLGYDTVEANCRLGFEPDLRDYGIGAQILSELGLSSIRLLTNNPKKIVGLEGYGLKVTERVRVAEATLPTKYGGEFKIIVYENEVDDMKHIALVKGDIQPEDEVLVRVHSECVTGDLFGSLRCDCGDQLHKAMEMVGQEGKGVIVYMHQEGRGIGLANKIRAYDLQEQGRDTVEANIELGFKEDLRDYGIGAQILVDLGVRKMRMITNNPKKIVGVEGYGLTVVERVREVVAQLPSEFGDFRIAAYTNLLDGKEHVALIKGEISSDEPILVRVHSECLTGDVFGSLRCDCGPQLHAALEQIEQAGSGVLLYLRQEGRGIGLINKLRAYNLQEQGFDTVEANEKLGFAADLREYGIGAQMLRDLGVRGMRLMTNNPRKIRGLEGYGLKVIERV",
    "response#2": "TAEAELPTDFGEFHMKVYESLTDGETHVALSKGDITSEESVLVRVHSQCLTGDVFASRRCDCGSQLHAAMKKVADEGCGVILYMSQEGRGIGLANKIRAYHLQDHGLDTVDANVKLGFAPDLRDYGIGAQILSDMGLKSIRLMTNNPKKIIGLSGYGLHVAERLVRLSATYRAADVLLPTDFGEFRAIAFENDVDRLEHLALVKGEIRGDEPVLVRVHSECMTGDVFGSLRCDCGSQLHRAMEMIEAEGRGVILYMRQEGRGIGLFNKLKAYALQDEGKDTVEANLALGFKPDLRDYGIGAQILVNLGVRNIRLLTNNPRKLIGLQGYGINIVERVREVVAQLPSEFGDFRIAAYTNLLDGKEHVALIKGEISSDEPILVRVHSECLTGDVFGSLRCDCGPQLHAALEQIEQAGSGVLLYLRQEGRGIGLINKLRAYNLQEQGFDTVEANEKLGFAADLREYGIGAQMLRDLGVRGMRLMTNNPRKIRGLEGYGLKVIERVPTRER",
    "response#3": "TAEAELPTDFGEFHMKVYESLTDGETHVALSKGDITSEESVLVRVHSQCLTGDVFASRRCDCGSQLHAAMKKVADEGCGVILYMSQEGRGIGLANKIRAYHLQDHGLDTVDANVKLGFAPDLRDYGIGAQILSDMGLKSIRLMTNNPKKIIGLSGYGLHVAERRAADVLLPTDFGEFRAIAFENDVDRLEHLALVKGEIRGDEPVLVRVHSECMTGDVFGSLRCDCGSQLHRAMEMIEAEGRGVILYMRQEGRGIGLFNKLKAYALQDEGKDTVEANLALGFKPDLRDYGIGAQILVNLGVRNIRLLTNNPRKLIGLQGYGINIVERVLEEGRRRVAEATLPTKYGGEFKIIVYENEVDDMKHIALVKGDIQPEDEVLVRVHSECVTGDLFGSLRCDCGDQLHKAMEMVGQEGKGVIVYMHQEGRGIGLANKIRAYDLQEQGRDTVEANIELGFKEDLRDYGIGAQILVDLGVRKMRMITNNPKKIVGVEGYGLTVVERVREVVAQLPSEFGDFRIAAYTNLLDGKEHVALIKGEISSDEPILVRVHSECLTGDVFGSLRCDCGPQLHAALEQIEQAGSGVLLYLRQEGRGIGLINKLRAYNLQEQGFDTVEANEKLGFAADLREYGIGAQMLRDLGVRGMRLMTNNPRKIRGLEGYGLKVIERVESVGGVRREVVAQLPSEFGDFRIAAYTNLLDGKEHVALIKGEISSDEPILVRVHSECLTGDVFGSLRCDCGPQLHAALEQIEQAGSGVLLYLRQEGRGIGLINKLRAYNLQEQGFDTVEANEKLGFAADLREYGIGAQMLRDLGVRGMRLMTNNPRKIRGLEGYGLKVIERV"
  },
  {
    "reference": "MVQRTFSKSRSETTKQIFVTGGVASSLGKGLTASSLGHLLRARGLTVTMQKLDPYLNVDPGTMNPFQHGEVFVTDDGSETDLDIGHYERFLDENLDGSANVTTGQVYSRVIEKERRGDYLGDTVQVIPHITDEIKRRMRLPSEADDAPDVIITEIGGTVGDIESQPFLESARQVRQDVGRNNVFFVHVSLVPYIGPSHELKTKPTQHSVAALRSIGIQPDALVIRSDREIPNEMREKLGRTCDVDPEAVINCADAPSIYDIPVKIHDQGLDAYIVQALGLKFKDVDWTQWTRLLHVVHHPDHELEVALVGKYIELPDAYLSVTEALRAGGFANGARVKIRWVPSDDCATDAGAKKALHGVDAICVPGGFGIRGLDGKLGALKFARENAMPTLGLCLGLQSMVIEYARNVAGLAEASSTEFEPDSVHPVIATMAEQQHIVDGKGDLGGTMRLGLYDAILGEGTVVAATYGTTEVAERHRHRYEVNNSYRKQLEDAGLVFSGTSPDGELVEFVELPADQHPYYVSTQAHPELSSRPTRPHPLFAGLIKAGLEHQKAGR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein's active site must be carefully engineered to ensure that the amino acids involved in H2O + L-glutamine = L-glutamate + NH4(+) are properly positioned for optimal catalysis.2. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.3. The presence of CTP synthase N-terminal domains is critical for the interaction of the designed protein with its intended targets.4. A protein should be able to manipulate the activity of enzymes in the CTP from UDP: step 2/2, Pyrimidine metabolism pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "VKVAIVGKYTQLEDAYKSINEALTHGGMANRVKVKIEWVDAELFDREDPAPHLEGFHAILVPGGFGERGTEGKIKAAEFARTRKVPYLGICLGMQMAVIEAARNVAGLADAGSEEFDHEAGKKRFEPVVYHLKEWVQGNAKVQRKADDDKGGTMRLGSYDATLVEGSKVAEVYGTTSIEERHRHRYEVDTKYRKQLEECGLLFSGMSPDGTLPEIVEWRDHPWFIGVQFHPELKSKPFAPHPLFEGFIRAAL",
    "response#2": "LADSYVSVNHALKHAGAKIGKAVSIDWIDSETINGNVDNLSKYDGVLVPGGFGSRGAEGIIKTANYTREKNIPYLGICFGFQLAAIAFGRYVCDFKDANSTELDSNTTNPIVDLLPEQKTVSNMGGSLRLGAHEITVKNNTLAHKTYNSDKVVRRHRHRYEINTKYIDAFEKNGMIFSAESDNSKRMEMLEIPAHKFYFGVQFHPEFNSRPGYPEEAFEAFIAV",
    "response#3": "LVESYKSLDEALRHGALAHQLKLVPVYVDSEDIEKEGADKLLKDVQGILVPGGFGERGTEGKISAIRFARENKVPFFGICLGMQLAIIEYARNICGLTDATSEEFNAGGDLLVHYMEGQTRDGAKGGSMRLGSYECELLDGSRARQMYGEKLIHERHRHRLEVNNFYVEQLKECGLVISGKNPELNLVETIEIKDHPFFFACQYHPEFKSRPYAAHPVFSEFILMG"
  },
  {
    "reference": "MDPTVGWIALLAFFVVSIALVVWGRNGF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in plasma membrane that is critical for cytochrome complex assembly. The designed protein sequence is ",
    "response#1": "LSNLYESFMFLSWSSSVIHIILEVRSQKDRGLGAITAPSTMLTHGFATSGLPKEMQQSAMLVPALQSHWLMMHVSMILLSYATLLCGSLSSIAFLIITFRRNRRILSLLSARDNSFIWPFPSRNNLYLHEQEKSDLQNTFYLSFTNHRKCKLTQQLDYWSYRVIGLGFLLLTIGILSGAVWANEAWGSRWSWDPKETWALITWIIFAIYLHTRVNKGWQDENPAIIASLGSFIVWICYLGVDLLGIGLLH",
    "response#2": "LSNLYESFMFLSWSSSVIHIILEVRSQKDRGLGAITAPSTMLTHGFATSGLPKEMQQSAMLVPALQSHWLMMHVSMILLSYATLLCGSLSSIAFLIITFRRNRRILSLLSARDNSFIWPFPSRNNLYLHEQEKSDLQNTFYLSFTNHRKCKLTQQLDYWSYRVIGLGFLLLTIGILSGAVWANEAWGSRWSWDPKETWALITWIIFAIYLHTRVNKGWQDENPAIIASLGSFIVWICYLGVDLLGIGLV",
    "response#3": "LSNLYESFMFLSWSSSVIHIILEVRSQKDRGLGAITAPSTMLTHGFATSGLPKEMQQSAMLVPALQSHWLMMHVSMILLSYATLLCGSLSSIAFLIITFRRNRRILSLLSARDNSFIWPFPSRNNLYLHEQEKSDLQNTFYLSFTNHRKCKLTQQLDYWSYRVIGLGFLLLTIGILSGAVWANEAWGSRWSWDPKETWALITWIIFAIYLHTRVNKGWQDENPAIIASLGSFIVWICYLGVDLLGIGLF"
  },
  {
    "reference": "AENEPSGETPCTELLVHVSTPGCDGFPSLDSKESYKLCVSKGSMLLSAETIWGALRGLETFSQLVGRDENGTYYINETEIVDFPRFPHRGLLLDTSRHYLPLRAILETLDVMAYNKFNVFHWHIVDDPSFPYESSTFPELSKQGAFNAMTHVYTASDVRSVIEHARLRGIRVIAEFDTPGHTLSWGPGAPGLLTPCYMGKVPSGVYGPINPIVNSTYKFVTRLFQEVSTVFPDFFLHLGGDEVDFTCWQSNPEIRAFMKEKGFGEDYKKLESFYIQRLLDIVSSLGKGYIVWQEVFDNDVKLRPDTIIHVWKDNNLQYLKEMANVTKSGYQALLSAPWYLNRISYGQDWIEAYKVEPLNFEGSAEQKALVMGGEACMWGEYVDVTNLTPRLWPRAGAVAERLWSNATVRDVEDAYARLAEFRCTLLRRGVQAQPLYVGYCDHEFGGV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein's Proton donor must be carefully engineered to ensure that the amino acids involved in ganglioside GM2 + H2O = ganglioside GM3 + N-acetyl-beta-D-   galactosamine are properly positioned for optimal catalysis.2. The designed protein should have beta-N-acetylhexosaminidase activity, N-acetyl-beta-D-galactosaminidase activity within membrane, lysosome to regulate hyaluronan catabolic process, neuromuscular process controlling balance, sexual reproduction, adult walking behavior.3. The designed protein have Beta-hexosaminidase eukaryotic type N-terminal, Glycoside hydrolase family 20 catalytic domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "TMTDRIGNGTHYTNNPAVAAYELRNPVTNGAFYVTIHADSTVGTDESFRLNVNTSAGALTVPSKGSIRLNGHQSKIIVTDFRFGPSHTLLYSTAEVLTHAVMDKKATLVLWVPTGESGEFAVKGAKSGKVERCPQCSNATFTRKKDVLVVNFTQAGGMSVLQLNNGVRVVLLDRAAAYKAVQNKHLFTNLNAYDFVWTVEKNGELVEKHASLLNVAPDGTDELTLSYPLYEQENETDEFVLTLSLRLSKDTAWASAGYEVAYEQFVLNFPHRGILIDTSRHFLSVKSIFKTLDAMAFNKFNVLHWHIVDDPSFPYQSVTFPGLSKKGSYSSSHVYTPNDVRMVIEYARLRGIRIVPEFDSPGHTQSWGEGQKDLLTPCYNGKEPSGAFGPINPIWNTTYSFLSKFFKEISTVFPDEFIHLGGDEVDFDCWASNPEIQDFMRDKGFGRDFKKLESFYMQAVLDMISAINKNSIVWQEVFDDASKLMPGPVIQVWKVEDYTQEQSKVTEAGFPVILSAPWYLDWISYGQDWKHYYAVDPLSFEGSQEQKKLVIGGEACLWGEYVDATNLSPRLWPRASAVGERLWSPRQRAWLTTHTGLLAIDVHSDYLFRQCDNEQLRWNIARDGEVLASGEVALTIAPQQTQRIEIDAPEFAAAAGEIWLNVDIVQTAATAWSPADHRCAWDQWQLYDDSGPAWVDANHMTTQNPRTPDTLPVLYADEYGFHNGIRLWRGSFTDAASGVYLNVQGGAAFGWSAYLNGHFLGS",
    "response#2": "FLHRGILIDTARHYLPVKTILKTLDAMAFNKFNVLHWHIVDDQSFPYQSITFPQLSNKGSYTLSHVYTPHDVRKVTEYARLRGIRVIPEFDTPGHTLSWGKGQKDLLTQCYSGKIPTDSDGPINPMLNTTYMFLTMLFQEISKVFPDQFIHLGGDEVEFNCWASNPDIQNFMKQKGFGQDFTKLESFYMQKLLDIIASMKKRSIVWQEVFDDKSELLPGTVIQVWKIRDYASKLSQVTASGFPAILSAPWYLDWISYGQDWRKYYLVEPLDFQGSDKQKKLVLGGEACLWGEYVDATNLTPRLWPRASAVGERLWSYDDSGPAWVDANHMTTQNPRTPDTLPVLYADEYGFHNGIRLWRGSFTDAASGVYLNVQGGAAFGWSAYLNGHFLGSVAAVQNKHLFTNLNAYDFVWTVEKNGELVEKHASLLNVAPDGTDELTLSYPLYEQENETDEFVLTLSLRLSKDTAWASAGYEVAYEQFVLVHIYDFSVRSSLDNNYEDGELSVSADILNYFEHDTQDLTFEVMLYDANAQEVLQAPLQTNLSVSDQRTVSLRTHIKSPAKWSAESPNLYTLVLSLKNAAGSIIETESCKVGFE",
    "response#3": "TMTDRIGNGTHYTNNPAVAAYELRNPVTNGAFYVTIHADSTVGTDESFRLNVNTSAGALTVPSKGSIRLNGHQSKIIVTDFRFGPSHTLLYSTAEVLTHAVMDKKATLVLWVPTGESGEFAVKGAKSGKVERCPQCSNATFTRKKDVLVVNFTQAGGMSVLQLNNGVRVVLLDRAAAYKAVQNKHLFTNLNAYDFVWTVEKNGELVEKHASLLNVAPDGTDELTLSYPLYEQENETDEFVLTLSLRLSKDTAWASAGYEVAYEQFVLIDVHSDYLFRQCDNEQLRWNIARDGEVLASGEVALTIAPQQTQRIEIDAPEFAAAAGEIWLNVDIVQTAATAWSPADHRCAWDQWQLLYDDSGPAWVDANHMTTQNPRTPDTLPVLYADEYGFHNGIRLWRGSFTDAASGVYLNVQGGAAFGWSAYLNGHFLGSEAHVFKSKSSCAAFLSNYNTSSAARVLFGGSTYDLPPWSVSILPDCKTEYYNTAKWLDLTMTDRIGNGTHYTNNPAVAAYELRNPVTNGAFYVTIHADSTVGTDESFRLNVNTSAGALTVPSKGSIRLNGHQSKIIVTDFRFGPSHTLLYSTAEVLTHAVMDKKATLVLWVPTGESGEFAVKGAKSGKVERCPQCSNATFTRKKDVLVVNFTQAGGMSVLQLNNGVRVVLLDRAAAYKL"
  },
  {
    "reference": "MSDAAKSNNNASADAPDPTTPDATGEADAANAATPTTPRGNHNNNNSANGRSKNKLKSTQNSSGGGSIDKLSPDQDIFINAADGLPNQHPSLPKEGDVDSDTEPEVDADSFDDLPTSIIVTNIHSEVFANPELKHAMEELFRTFSESATFQWLRSFRRLRVNYDNAIAAANARIKLHQYEFNKKTVITCYFAQPVTPVSNKNLQPPAPVKQFLISPPASPPAGWEPREEGEPLVNHDLLAALASLTPGESHELHPQSEDQPAIIVHTAMLAETGPGLQVKAPIVQTKCPERA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.2. A protein that can perform calcium-dependent protein serine/threonine phosphatase regulator activity in mitochondrial matrix, cytoplasm, nucleus to support regulation of female receptivity, post-mating, regulation of calcineurin-NFAT signaling cascade, sleep.3. The protein must exhibit the following characteristics: Critical for ovulation, meiotic progression in oocytes and female courtship behavior, including their postmating changes; Required for elongation of meiosis I spindle; Has a role in calcium signaling during egg activation; bcd mRNA polyadenylation and translation in the oocyte.; Regulates female meiosis by controlling calcineurin activity in the germline. The designed protein sequence is ",
    "response#1": "EFVQEFNRLDRMFVSNELSDEESLQTAFNANYLTKARSKMVPCAEFSRVKLNDGLGRLDDRNELRNGMFSDEHEFLQEEGYTAKSTYGTTDFIHANYVKGGPLLNTFICAQAPLKNTQEDFWRMVFQEKCQFIVMLNSAVDSSTLGPLDSANRNHCPYYWPRAENESLRFGSFHITCMKVDSKADPLFTITKLKVQKVGGNLLDAEFDEELFLEHWQWDWQYLGDVHWPFRVLRKARQLSTPTIVQCIDGCSKSGTLVSIETALMHFIRGSPITKSLILQSCVFVRLQRRLSVSSVLLYLFIYRVILRWIY",
    "response#2": "FVQEFNRLDRMFVSNELSDEESLQTAFNANYLTKARSKMVPCAEFSRVKLNDGLGRLDDRNELRNGMFSDEHEFLQEEGYTAKSTYGTTDFIHANYVKGGPLLNTFICAQAPLKNTQEDFWRMVFQEKCQFIVMLNSAVDSSTLGPLDSANRNHCPYYWPRAENESLRFGSFHITCMKVDSKADPLFTITKLKVQKVGGNLLDAEFDEELFLEHWQWDWQYLGDVHWPFRVLRKARQLSTPTIVQCIDGCSKSGTLVSIETALMHFIRGSPITKSLILQSCVFVRLQRRLSVSSVLLYLFIYRVILRR",
    "response#3": "FVQEFNRLDRMFVSNELSDEESLQTAFNANYLTKARSKMVPCAEFSRVKLNDGLGRLDDRNELRNGMFSDEHEFLQEEGYTAKSTYGTTDFIHANYVKGGPLLNTFICAQAPLKNTQEDFWRMVFQEKCQFIVMLNSAVDSSTLGPLDSANRNHCPYYWPRAENESLRFGSFHITCMKVDSKADPLFTITKLKVQKVGGNLLDAEFDEELFLEHWQWDWQYLGDVHWPFRVLRKARQLSTPTIVQCIDGCSKSGTLVSIETALMHFIRGSPITKSLILQSCVFVRLQRRLSVSSVLLYLFIYRVILRK"
  },
  {
    "reference": "MRLAALLDDAEALAGDPVVTGLAIDHRKVAPGTIFGAFVGEKFNGEDFIPAAVAAGAIAVVARPEAQVTGALHIADANPRRAFAHIAARFFHRFPATCVAVTGTNGKTSTVEMTRQLWRMAGFHAASIGTLGVTTGDDRVVTGLTTPDIVTFLSNMAGLAAEGVTHAAFEASSHGLDQYRTEGLPVKAAAFTNLSHDHLDYHGDMYAYLAAKMRLFRDVVDADGTAVIWADDTMWAPAAEYEAQQRGLKMLTVGREGHDLRLVSHEPTQLGQSLMIQCGPLVQKVTLPLIGAYQVANALVAAGLVIATGGDPAQTLGNLARLQPVRGRLERAAITRAGAPVYVDYAHTPDAIEAALDALRPHVAGRLILVFGAGGDRDQAKRAEMGRIAAAKADLAIITDDNPRGEDPATIRAAIAEGAPAARIIGDRRAAIAAAIAEAGADDVVCIAGKGHEQGQIVGRGEAMRVIPFDDVTVVREEAA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The presence of Mg(2+) is necessary for the protein to function in the desired environment.2. The designed protein should have a high specificity for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP, meso-2,6-diaminoheptanedioate binding, with minimal off-target effects.3. The protein's functional design necessitates the inclusion of a Meso-diaminopimelate recognition motif motif.4. The protein should be designed to catalyze the following reaction ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.5. The protein should be optimized for activity in peptidoglycan biosynthesis, Cell wall biogenesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.6. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan. The designed protein sequence is ",
    "response#1": "MKLGELLSPWLPELTLQTDSRRVSPGDGFIAWPGAATDGRAHVGDALARGAAACLVEQEDSLQTDSRRVSPGDGFIAWPGAATDGRAHVGDALARGAAACLVEQEGWRVLGEWNPALPAITGTNGKTTTTQLVAQWAQLLGETAAVMGTVGNGLLGKVIPTENTTGSAVDVQHVLSDIRAQGATVAAMEVSSHGLVQDRVAALQFAAAVFTNLSRDHLDYHGTMENYAEAKWRLFSTHQFGQAIINADDAIGREWLTRLPHAVAVSMQPTLPLAWDGRFLCVTEVAYHPQGARIAFRSSWGDGQIDSPLMGAFNVSNLTLALASLLALGYELDTLMTAAPALQPVCGRLEFAGRSGDAPVFIDYAHKPDALSKTLEALRPYVRKRLVVVFGAGGNRDPGKRAMMGAIAAEKADKVIVTDDNPRNEDAGTIRAAIMAAAPGAIEIGDRGEAIRQAIATLESGDVLVVAGKGHEDYQIIGNEKLHFSDLEVAAAALGVTLQTDSRRVSPGDGFIAWPGAATDGRAHVGDALARGAAACLVEQEGWRVLGEQNPALAARTIGITGTNGKTTTSHLIEHVMLHSGWRALGEENPSLAARRIALIGVTGTNGKTTTTMLVRHLLGEDWRLLGDTNPALAARTIALIGVTGTNGKTTTTMLVRHLLGEDWQVLGEQNPALAARTIALQVTGTNGKTTITQIIAQWLNLVGKRAAVLGTAGNGFLDALQPAVNTTGSAIEIQQTLVSIESQGADFTAMEISSHGLVQGRVKTLPFAAGVFTNLSRDHLDYHGTMEEYELAKRSLFTEHQCGKAIINVDDQVGAKWAEQLPDAIQVSLQPLSSTNAVFASQVDYSESGISLAFSGRFGDGQFSVPLIGEFNANNVLVAFT",
    "response#2": "MTLDSRTAAAGDLFIAVLGHKTDGRRYIAQAVAQGVAAVIAEAEGEASDGCIREVHGVPVVYLSQLNRKLSALAGRFYGFPSRSLKLVGITGTNGKTTVTQLINQWSELLGNKIATMGTLGNGFYKSLKPTKNTTSSAIDIQSFLHSVLDKVNFVTIEVSSHGLSQNRVKNIFFHTAIFTNLTQDHLDYHKNMKKYELAKRSLFTDHKVKKIILNANDTYAKNWLKTFSNKYTVAVTIKDHKQKKYSTKWINAIHIKVYNEKTDVKFESSWGSGILSTCLIGKFNIENLLLALATLLASGLSLQESVNAVSRLQPVCGRLEFAGRSGDAPVFIDYAHKPDALSKTLEALRPYVRKRLVVVFGAGGNRDPGKRAMMGAIAAEKADKVIVTDDNPRNEDAGTIRAAIMAAAPGAIEIGDRGEAIRQAIATLESGDVLVVAGKGHETYQEISGQRLPFDDRQWARDWLRQRG",
    "response#3": "MKLRQLLEGLDFTCLAGTENLQTDSRRVSPGDGFIAWPGAATDGRAHVGDALARGAAACLVEQEVPLAEITFICQDSRQVSSGCMFAALPGTQTDGHLFIRQAAQKGASAILFQKECLTDLQVIQEFSNNTTFIQVPDTHYALGLLADAWYPRQPDLLAIGVTGTNGKTTTTMLVRHLLGEDKPAAQLGTVRYRYADAAYRSPELTTPDPLTMQQLLAAMRQAVAAGCRYAAMELSSHALEQGRVNGVAFAAAVFTNLTRDHLDYHGDMEAYGAAKARLFTREGLAFAVVNADDPYGRRLVAQAAAEAGADGAGPKVLTYGLGEGADVRASDIRMEAGGASFLLHTPWGEGEVRSRLLGEFNVENVLAAVAVCAALGVPAEAIVPALGTVKAVPGRMEQVALPGQPLVAVDYAHTPDALDQALQALAPVATARGGRLWCVFGCGGNRDAAKRPLMGGVAQQRADQVIVTSDNPRGEDPAEIIRQILQGTIAGLTVRAEPDRATAIATALQEAGADDVVLIAGKGHENYQEIAGVRRPFDDAAEARAALAARGVLA"
  },
  {
    "reference": "MFQYNKVEEALAALKAGEMIIVSDDENRENEGDLICAAEFISPEIINFMASEAKGLICAPLSETYAKNLQLEAMVEKNTDNHGTAFTVSVDHIETSTGISAFERALTIKKLVDAESLPSDFRRPGHVFPLIAKKNGLFERNGHTEATVDLLRLAGLKEVGVCVEIMDEDGTMMHKEKLKEKAQEWGLKYITIKAIQEYLKQNELQVEQVTRAKLPTKYGMFDILGFVNKINGEHHVALVKGDIGDGQAILCRVHSECLTGDAFGSLKCDCGGQLEEALKKINDEGRGVLLYLRQEGRGIGLINKLRAYSLQDEGLDTVEANLALGFEEDEREYSIGAQILKIIGVKSLKLMTNNPQKINDFQKYGLVVEERVALQIKDNPFDLDYLKVKQNKMGHLFD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.2. The designed protein should have a binding site that is specific to the target D-ribulose 5-phosphate, GTP, Zn(2+), Mg(2+) ligand.3. The protein with the reaction GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate, the Nucleophile; for GTP cyclohydrolase activity, Proton acceptor; for GTP cyclohydrolase activity must be optimized to ensure efficient catalysis.4. The designed protein must possess GTP binding, zinc ion binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, manganese ion binding, GTP cyclohydrolase II activity to support riboflavin biosynthetic process under certain conditions.5. Zn(2+) is required for the proper folding and stability of the protein.6. The protein contains novel GTP cyclohydrolase II domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "VAKSNLPTKYGMFELYGFKDLVTGEHHEALVMGDIADGDPVLCRVHSECLTGDTFGSLKCDCGQQLDLALKKISEEGRGVLVYLTQEGRGIGILNKIKAYALQDKGMDTVDANLALGLPEDARDYNVGIQILRDLGVSKIKLMTNNPKKIYGLGDKNCGLEITERIPYIDIQTKMKVVETSIPTAHAGLFKVIVYENDLEDHQHIALIKGEIDPEKPILVRVHSECLTGDIFHSLRCDCGDQLAKAMEMMDKEGSGVLLYVRQEGRGIGLVNKLKAYVLQDKGYDTVEANEKLGFKADLRDYGIGAQVLADLGVKQMRLITNNPKKIVGLEGYGLSIVEQVPYIDIQTKMT",
    "response#2": "VRVDMPTEYGHFELVAFKEKNNKAEHLALIKGDIGNGEPVLTRVHSSCFTGDILGSLRCDCGDQLHAAMQMVEKEGRGLILYMNQEGRGIGLINKLRAYKLQEQGYDTVEANLELGFGVDERDYGVGAQILRYLGATKLRLMSNNPRKRAGLNGYGIEIVETVQPADAYRLYRKETVKLPTEFGIFDLHCFVAKNDGREHLALVYGEVAGQENVLTRVHSECLTGDVFHSRRCDCGQQLAAAMRQVVANGSGVVVYMRQEGRGIGLVNKLHAYRLQEKGLDTVDANIKLGFPPDLREYGIGAQILANLGVKSIRLLTNNPKKIVGLQGYGLQVTGRSYARRGKLTWLPEANFPTRFGAFRLYLFKDKLSGEEHIALIKGDIKGKKNVLVRVHSQCFTGDVLGSLRCDCRDQMEKALKIINDEGQGVFLYMRQEGRGIGLSNKVHAYHLQDLGYDTVEANCRLGFEPDLRDYGIGAQILSELGLSSIRLLTNNPKKIVGLEGYGLKVTERVPMASLVDG",
    "response#3": "RAVESKLPTAHGEFKLIAYETIVDQQNHMAFVKGDVADGEPVLVRVHSQCATGDTFGSLRCDCGHQLETALRMIEKEGRGVLIYLMQEGRGIGLINKLKAYNLQDEGFDTVEANEKLGFKADLRDYGIGAQILQDLGVRKMRLLTNNPKKIVGLEGYGLEIVERLLRLPEPLPDVRVDMPTEYGHFELVAFKEKNNKAEHLALIKGDIGNGEPVLTRVHSSCFTGDILGSLRCDCGDQLHAAMQMVEKEGRGLILYMNQEGRGIGLINKLRAYKLQEQGYDTVEANLELGFGVDERDYGVGAQILRYLGATKLRLMSNNPRKRAGLNGYGIEIVETVREAVAKMPTKYGNFNIVGYQNKTTGEHHVALTMGDVGNGEPVLCRVHSECLTGDVFGSKRCDCGQQLEKAMEMVAKEGRGIILYLRQEGRGIGLINKLKAYELQEQGLDTVDANLKLGFPADMRSYGMGAQILADLGAKKLRIMTNNPAKITGLSGYGIEIVERVREARTHLPTAFGAFTAHGYRSTADGVEHVALVHGDIGDGQDVLVRVHSECLTGDVFHSLRCDCGPQLETSLQRIAAEGRGVVVYLRGHEGRGIGLLSKLRAYELQERGSDTLDANLELGLPADARDYGAGARILEDLGVRSVRLMTNNPEKTDALIHHGLTVTGRR"
  },
  {
    "reference": "MDYLPIFCELKNRDCLLVGGGDVAERKARLLMDAGANVIVNAREFTPQFTVWAEHKQLSLVKGDFSPALMDNCWLVIAATDDDQTNQHVSQEAEQRKLFCNVVDAPKRASFIMPSIIDRSPIMVAVSSGGTAPVLARLLREKLEAVLPQHLGKVAQFAGQLRQRVKDTFTTMAERRRFWEKLFANDRLAQSLANNDQVAIHSQTETLFSTPLDNQGEVVLVGAGPGDAGLLTLKGLQQIQQADIVVYDRLVSDDVMKLVRRDADRVFVGKRAGYHCVPQEEINQILLREAQSGKRVVRLKGGDPFIFGRGGEELETLCEAGIPFSVVPGITAASGCSAYSGIPLTHRNYAQSVRLVTGHLKSSGEELDWASLAAEKQTLVFYMGLNQAPAIEKNLLAHGMPAETPCALVENGTATTQKVVQGTLDKLAELAQQVQSPSLIIVGRVVGLREKLNWFSNH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions.2. The protein must exhibit the following characteristics: Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1.3. A protein that can modulate the overall flux of metabolites through the Porphyrin-containing compound metabolism pathway, enhancing or suppressing its activity as needed.4. The protein contains novel Sirohaem synthase dimerisation domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "VGAGPGNPDLLTFRALRLMQQADVVLYDKLVAPELLELVRRDAERIYVGKARADHTLPQDDINSLMVSLAKAGRRVLRLKGGDPFTFGRGGEEIDTLAAEGIPFEVVPGITSASGAAAYAGIPLTHRDYAQSVVFVTGHMKDGSFNLDWPALTRPQQTVVVYMGLSHIQGLCDGFILHGKAADTPAAIVEQATTVRQRTLTGTLADLPALAQAHGIASPALIIVGEVGAGPGDPGLLTLRGLRALNEADVILHDRLVSVEVLALARRDAERIEVAKQAGNHHTTQAQINALMLEHASAGRRVVRLKGGDPFVFGRGGEELEFLRSHGIPYEVVPGITAALACAAYAGIPLTHREYAQSVRLVTAHCQNSNDTLDWRALAQERQTLAVYMGTSELPVIRSRLLEHGRSAGTPFALVENGSRPEQRVVTGTLANLVEQASLHDVRSPALLIIGEVARRRG",
    "response#2": "VGAGPGDPDLLTFRALRLMQQADVVLYDRLVAPAILELCRRDAERVYVGKRRADHAVPQDQINQQLVDLARQGKRVLRLKGGDPFIFGRGGEEIEELAAHGIPFQVVPGITAASGCAAYAGIPLTHRDYAQSVRFITGHLKNGTSDLPWQDLVGPSQTLVFYMGLIGLPIICEQLIKHGRAADTPAALIQQGTTSNQRVFTGTLADLPRMVAEHEVHAPTLVIVGEVL",
    "response#3": "VGGGPGDPDLLTFRALRLMQQADVVLYDRLVSPAVLELVRRDAERINVGKARSDHTLPQEDINTLLVRLAKAGRRVLRLKGGDPFIFGRGGEEIETLAAEGIPFQVVPGITAASGCASYAGIPLTHRDHAQACVFVTGHMKHGVLDLNWTALAQPRQTIVFYMGLHGIEQIRDGLVGHGLSPDTPAAVVEQGTTAQQRVHVATLATLPERVRDAGIRPPSLIIVGSVVGAGPGDAGLLTLKGLQQIQQADIVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHCVPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCQAGIPFSVVPGITAASGCSAYAGIPLTHRDYAQSVRLVTGHLKTGGELDWQNLAAEKQTLVFYMGLNQAAAIQQKLLEHGMPSDMPVAIVENGTAIKQRVVDGVLTGLAALATQMESPALIIVGRVE"
  },
  {
    "reference": "MKLNKKERQKLLQQTISSTPFITDEELATKFGVSIQTVRLDRLELSIPELRERIKHVAEKTLEDEVKSLPLDEVIGEMIDVELDDQAISILEVRKEHVFSRNQIARGHHLFAQANSLAVAVIDDELALTAKANIKFTRQVKQGERVVSKAKVASHDKEKGRTVVEVNSYVGEEVVFSGDFVMYRSKQK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must possess DNA binding to support negative regulation of DNA-templated transcription, negative regulation of fatty acid biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "ETLLQQVARLLAVNTNYATMVTSPQYRSRKVKFLQVSEVDTTQILTIIVLEGNIVKNKIIAIAEPLDKETLLKLNIVLNTFLQGLDLSEINLALIRQMKEQAAEHSSVVSDILDAVAQAISEEDDIQIFTSGATNILKYPELNDMEKASELLYTLEEKKQLTNLMNNEVSSGENRGIQVYIGNETPVDSMKDCAVVTATYQIEEGVYGKIGIIGPKRMDYEKVL",
    "response#2": "METLASRLDACQETLLELYEKDSNKLEDQIKHWAQVRLENVMLFKARECGMTRVGCTTVPALTVSKAKACQAIEVQLALQTLMQSAYSTEAWTLRDTCLEMWEAPPKRCWKKKGQSVLVKFDGSCDRDMIYTGWGHIYVQDINDDTWHKVPGQVDELGLFYVHDGVRVNYVDFGIEALTYGVTGTWEVQVGGRVIN",
    "response#3": "METLASRLDACQETLLELYEKDSNKLEDQIKHWAQVRLENVMLFKARECGMTRVGCTTVPALTVSKAKACQAIEVQLALQTLMQSAYSTEAWTLRDTCLEMWEAPPKRCWKKKGQSVLVKFDGSCDRDMIYTGWGHIYVQDINDDTWHKVPGQVDELGLFYVHDGVRVNYVDFGIEALTYGVTGTWEVQVGGRVIT"
  },
  {
    "reference": "MVSQNYLGVMSGTSLDGVDLALVDFSQTPKLIAADFTPMPDTLRVALSQLVKKGETSLQALGELDHQLALLYADSIQRFLAKHQLNADDIVAVGCHGQTVWHSPKGQYPFTMQIGDMNLLAARTGITTVGDFRRRDMAFGGQGAPLVPAFHQAVFSSPERLTVVLNIGGISNISVLNPNQQTIGYDVGAGNTLLDIWIEKHLGKRYDANGEWARTGKIHSALLALLLDEPFFQLPPPKSTGRELFNLEWLEHKLTQIKPLPAEDVQATLVEFTVQSIVQALRQIENPQAYPCVLLVCGGGAKNPLIMERLQTHLTDWQVSTTTEYGLDVDYVEAAAFAWLAYQRIHNLPANLPSVTGATQAVSLGAIFPNI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.2. The designed protein should be optimized for kinase activity, ATP binding activity in order to improve phosphorylation, 1,6-anhydro-N-acetyl-beta-muramic acid catabolic process.3. The protein must exhibit the following characteristics: Is required for the utilization of anhMurNAc either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling.; Catalyzes the specific phosphorylation of 1,6-anhydro-N- acetylmuramic acid (anhMurNAc) with the simultaneous cleavage of the 1,6-anhydro ring, generating MurNAc-6-P.4. A protein should be able to manipulate the activity of enzymes in the peptidoglycan recycling pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "DVAGFALTEEERERLLDPLVGGVILFARNFRDSEQLQALTAEIHALRSPALIIAVDHEGGRVQRFRTDGFTRIPSMRCLGRLWERDHVAALESARCAGYVLAAELLAHGVDLSFTPVLDLDYGCSRVVGDRAFHRDPLVVAALAQSLVSGMADAGMGCVGKHFPGHGYAEADSHVEIPVDEREFDAIWTEDIAPYRHRLGRQLAGVMPAHVIYPRVDPNPAGFSRFWLQDILRGRVGFGGVIFSDDLTMEGATVVGDILARARAAFGAGCDVVLVCNREALVPV",
    "response#2": "MAEDVAGFALTEEERERLLDPLVGGVILFARNFRDSEQLQALTAEIHALRSPALIIAVDHEGGRVQRFRTDGFTRIPSMRCLGRLWERDHVAALESARCAGYVLAAELLAHGVDLSFTPVLDLDYGCSRVVGDRAFHRDPLVVAALAQSLVSGMADAGMGCVGKHFPGHGYAEADSHVEIPVDEREFDAIWTEDIAPYRHRLGRQLAGVMPAHVIYPRVDPNPAGFSRFWLQDILRGRVGFGGVIFSDDLTMEGATVVGDILARARAAFGAGCDVVLVCNRRSTADEGPEALAGVVAAALRAGGRLIYIGAGTSGRLGILDASECPPTFGTRPEQVVGIIAGGHKAILSAVENVEDNKAQGAMDLQNLNFSNRDVLVGLAASGRTPYVIGAMEYAHSQNAFVAIVSCNPHGEMAQLADVAITPVVGPEVVTGSTRLKAGTAQKLVLNMISTGAMIRIGECTGIGASLRDVAAALRAGGRLIYIGAGTSGRLGILDASECPPTFGTRPEQVVGIIAGGHKAILSAVENVEDNKAQGAMDLQNLNFSNRDVLVGLAASGRTPYVIGAMEYAHSQNAFVAIVSCNPHGEMAQLADVAITPVVGPEVVTGSTRLKAGTAQKLVLNMISTGAMIRIGDRLVGYPLAELSPEVAAALRAGGRLIYIGAGTSGRLGILDASECPPTFGTRPEQVVGIIAGGHKAILSAVENVEDNKAQGAMDLQNLNFSNRDVLVGLAASGRTPYVIGAMEYAHSQNAFVAIVSCNPHGEMAQLADVAITPVVGPEVVTGSTRLKAGTAQKLVLNMISTGAMIRIG",
    "response#3": "MNDVAGFALTEEERERLLDPLVGGVILFARNFRDSEQLQALTAEIHALRSPALIIAVDHEGGRVQRFRTDGFTRIPSMRCLGRLWERDHVAALESARCAGYVLAAELLAHGVDLSFTPVLDLDYGCSRVVGDRAFHRDPLVVAALAQSLVSGMADAGMGCVGKHFPGHGYAEADSHVEIPVDEREFDAIWTEDIAPYRHRLGRQLAGVMPAHVIYPRVDPNPAGFSRFWLQDILRGRVGFGGVIFSDDLTMEGATVVGDILARARAAFGAGCDVVLVCNRPTMPFEMDVAAAAMDAYRMARDAS"
  },
  {
    "reference": "MLRGNDTGSDLAELLWDNGAPAPLRPPPPPPFQPFTCSAAATTSPPAHDYLFIKNLMRGGGAANHHHHDDDDDDDDDVPWLHYHPVVDDDDDADADTAPLPPDYCAALLSGLSDHLPPPAAAASRVDPDPCSSSHGAVVPSTSAAAAKQARTSGGGGGGVMNFTFFSRPLQQRPSGGETASASASAAATSTVPVESTVVQAATNRLRSTPLFSDQRMAWLHPPKPSPRAAAPPPPPPLAPTTRHRLDTAAATATVAQRLPPSEARAPDAPPPAATATATTSSVCSGNGDRRQLNWRDSHNNQSAEWSASQDELDLDDELAGVHRRSAARSSKRSRTAEVHNLSERRRRDRINEKMRALQELIPNCNKIDKASMLEEAIEYLKTLQLQVQMMSMGTGMFVPPMMLPAAAAAMQHHHMQMQQMAGPMAAAAHFPHLGAAAAMGLAGFGMPAAAQFPCPMFPAAPPMSMFAPPPPPPPFPHAAATAVEQTPSPPGAADAGNAPAVKQA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein contains novel bHLH domains that confer a unique function or activity.2. The ideal protein would be one that plays a critical role in regulation of seed growth through protein dimerization activity, DNA-binding transcription factor activity within nucleus.3. For general function, the protein need meet that May be involved in the control of lamina inclination through brassinosteroid signaling pathway.4. The designed protein's functionality will benefit from Pro residues, Basic and acidic residues, Polar residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "AAMFDMEYARWLDDDTKRLAELRGGLQAHLLDGNLGLIVEECMQHYDELFQLKAALARSDVFHLLTGSWATPAERCFFWMGGFRPSELLKILIPQLDPLTEQQLLGICNLQQSSEQAEEALAQGLHQLHQSLADTVAAGTLNDGAAAPNYMNIMAVALEKLASLENFYQQADNLRHQTLHQMRRILTTRQAARCFLSIGEYYSRLRALSNLWASA",
    "response#2": "WKQQRKESHKEVERRRRQNINTAIEKLSDLLPVKETSKAAILSRAAEYIQKMKETETANIEKWTLQKLLGEQQVSSLTSANDKLR",
    "response#3": "AAMFDMEYARWLDDDTKRLAELRGGLQAHLLDGNLGLIVEECMQHYDELFQLKAALARSDVFHLLTGSWATPAERCFFWMGGFRPSELLKILIPQLDPLTEQQLLGICNLQQSSEQAEEALAQGLHQLHQSLADTVAAGTLNDGAAAPNYMNIMAVALEKLASLENFYQQADNLRHQTLHQMRRILTTRQAARCFLSIGEYYSRLRALSNLWASG"
  },
  {
    "reference": "MRVMGIQMNCQNWWVILGMLIIYSVAENLWVTVYYGVPVWKDAETTLFCASDAKAYDTEAHNVWATHACVPTDPNPQEIHLENVTEEFNMWKSNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLDCHNVTNNFTEGVPEEEIKNCTFNMTTELRDKKQSVYSLFYRLDIVQIGGKNNSQHYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCRDTKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVVRSENITNNVKPIIVQLVRPVNITCVRPNNNTRKSVRIGPGQAFYARGGIIGDIKQVYCIVNETEWKGALRTVSLQLGNYFKDKAINFTNSSGGDVEITTHSFNCGGEFFYCNTSGLFNSLWINGTMLNGTLKNSPSTNDTITLPCRIKQIINMWQKVGQAMYAPPIPGSITCVSNITGLILTRDGGNTNRTDEIFRPEGGNMRDNWRSELYKYKVVKIEPLGVAPTRAKRRVVEREKRAVGLGAVFIGFLGAAGSTMGAASITLTVQARNLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNKSQSEIWNNMTWMQWDKEIDNYTQEIYRLIEESQNQQEKNEQDLLALDKWASLWTWFDISNWLWYIRIFIMIVGGLIGLRIVFAVLSVINRVRQGYSPLSFQTHTPNPGGPDRPGRIEEEGGEQDRNRSIRLVSGFLALAWDDLRSLCLFSYHRLRDFILIAA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should include a Di-leucine internalization motif, YXXL motif; contains endocytosis signal motif to ensure proper function.2. The protein contains novel Human immunodeficiency virus 1 envelope glycoprotein Gp120, Retroviral envelope protein GP41-like domains that confer a unique function or activity.3. Include a signal peptide as an essential component of the protein.4. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.5. A protein that can perform structural molecule activity in viral envelope, host cell endosome membrane to support mitigation of host antiviral defense response, clathrin-dependent endocytosis of virus by host cell, positive regulation of receptor clustering, positive regulation of establishment of T cell polarity.6. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "MWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPSPQELYLKNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLDCTKVNVSANQTIIRFNQNVNDSEYEEMKNCSFNMTTELRDKQHKVHALFYKLDIVNLGNDSDSSSYRLINCNTSAITQACPKVSFDPIPLHYCAPAGYAILKCNNKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEGDIIIRSENLTDNVKTIIVHLNESVEINCTRPNNNTRKGIGIGPGQTFYATGAIIGDIRQAHCNISEYKWNETLQRISKKLKEHFPNKTIEFKPASGGDLEITTHSFNCRGEFFYCNTSNLFNRTFNFNDTDTFNSTNENNRTANITLQCRIKQIINMWQEVGRAMYASPIAGSIVCNSNITGLLLTRDGGNSGKNDTETFRPGGGDMRNNWRSELYKYKVVEIKPLGIAPTDAKRRVVEREKR",
    "response#2": "MWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPSPQELYLKNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLDCTKVNVSANQTIIRFNQNVNDSEYEEMKNCSFNMTTELRDKQHKVHALFYKLDIVNLGNDSDSSSYRLINCNTSAITQACPKVSFDPIPLHYCAPAGYAILKCNNKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEGDIIIRSENLTDNVKTIIVHLNESVEINCTRPNNNTRKGIGIGPGQTFYATGAIIGDIRQAHCNISEYKWNETLQRISKKLKEHFPNKTIEFKPASGGDLEITTHSFNCRGEFFYCNTSNLFNRTFNFNDTDTFNSTNENNRTANITLQCRIKQIINMWQEVGRAMYASPIAGSIVCNSNITGLLLTRDGGNSGKNDTETFRPGGGDMRNNWRSELYKYKVVEIKPLGIAPTDAKRRVVEREKRAALGLLLGTALPSSXGXEXLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPSPQEVVLENVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDWRNATNINATQITNGTLEGGEVKNCSFNITTRLRDKVQKKYALFYTLDVVPIREDNSNTSYRLVSCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNNETFDGKGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEKGIVLRSENFSDNAKTIIVQLTTPVKINCTRPNNNTRRSIHLGPGKAIYTTGEIIGDIRRAHCNISRKEWNDTLTQIVTKLREHFKNKTIVFNQSSGGDPEIVMHTFNCGGEFFYCNSTQLFNSKWEGNSTWNSTGESNNTAENIILPCRIKQIVNMWQEVGKAIYAPPISGPIRCSTNITGLLLTRDGGKDENQTEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREK",
    "response#3": "MWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPSPQELYLKNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLDCTKVNVSANQTIIRFNQNVNDSEYEEMKNCSFNMTTELRDKQHKVHALFYKLDIVNLGNDSDSSSYRLINCNTSAITQACPKVSFDPIPLHYCAPAGYAILKCNNKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEGDIIIRSENLTDNVKTIIVHLNESVEINCTRPNNNTRKGIGIGPGQTFYATGAIIGDIRQAHCNISEYKWNETLQRISKKLKEHFPNKTIEFKPASGGDLEITTHSFNCRGEFFYCNTSNLFNRTFNFNDTDTFNSTNENNRTANITLQCRIKQIINMWQEVGRAMYASPIAGSIVCNSNITGLLLTRDGGNSGKNDTETFRPGGGDMRNNWRSELYKYKVVEIKPLGIAPTDAKRRVVEREKRXMGFGAXLY"
  },
  {
    "reference": "MYMANIAIAGATGAVGQEILNSLAKKEFPVNELKLFSSSRSAGNTQATSFGPYILEEFSIEACKNIDIIFFAVGGDFSRKYASELLEKNDKIIIIDNSSAFRYDKNIPLVVPAINASAIKNSRLIANPNCTTAILAMVLHPIYKEFGLKKCIVSTYQATSGAGNPGMQELLHESKNYIDGLPVENKIFSHPIPFNLIPHIDVEQENHYTKEEMKVTWETHKIFEDDSFPLSCTAVRVPIIRAHSESVVIETKKNITENGGLEKVREILINAVGVTLRDDIQNNNYPMPLNASEIDEVQVGRIRKNLVFGDFGLEFFVCGDQLLRGAALNAVEIAELVKI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must have at least one Semialdehyde dehydrogenase NAD-binding domain.2. A key design requirement for the protein is the optimization of the Acyl-thioester intermediate, Proton acceptor to achieve high catalytic efficiency for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.3. We require a protein that can specifically bind to the metabolites produced in the L-threonine biosynthesis, L-threonine from L-aspartate: step 2/5, Amino-acid biosynthesis pathway.4. The protein design must be able to perform NADP binding, protein dimerization activity, aspartate-semialdehyde dehydrogenase activity in order to contribute to threonine biosynthetic process.5. The phosphate, substrate binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MGYDVAVLGATGAVGRTIIEVLEERKFPVRNLHLLASERSAGETIRFNGKSVRVKNVEDFDWSQVQIALFSAGAESSDRWAPIAADEGVVVIDNTSRFRYEYDVPLVVPEVNSEAIADALARGVKNYIGGNCTVSLMLMGALHGQYGLRELVVASYQAASGAGQAGIDTLYDQLEKVGGNRGLGERAGDVRRVVGEDLGPFPAPLAMNVVPWAGSLKDDGWTSEELKVRNESRKILGLPDLRVSATCVRVPVVTTHSLAVHAVFDRPVDQREAQAVLAGSPGVVLVDDPEAGEFPTPADVVGTDPTWVGRVRRSLDDPKALDLFLCGDNLRWGAAEPIRRMLLIINR",
    "response#2": "MASVGLIGWRGMVGSVLMQRMTEERDFDVIRPVFFSTSQHGQAAPQFGGQSPGLLQNAYDLDALKALDIIITCQGGDYTTEIYPKLRAAGWQGYWIDAASTLRMQDDALIILDPVNHHVIRQALAKGGRNWIGGNCTTNCMLMGMGVGAPLHHKFVIRRLVISTYQSVTGSGIKGQHQLENEQKGLSVVNSAYPHPIHMNVIPHGGDFDESGSTSEEQKLVFETRKIMDAPDIAISATVVRVPVTGGHSMSVNIEFEKKFDLQEVRQLLESSPGLKVQDNPAENYYPMPLYAQNKDEVFVGRIRRDESISNGLNMWIVADNLRWGAAEPLRRMLRILLER",
    "response#3": "MTRVGLVGWRGMVGSVLMERMVEEKDFDLIDPVFFTTSQVGGKGPAIGRDAPVLQDARSVDSLKAMDIIISCQGGDYTTEVFPKIRAAGWNGHWIDAASTLRMQDDAVIILDPVNLNVIKDAIARGVKDFIGGNCTVSLMLMAWGAAEPLRRMLRILQDQIG"
  },
  {
    "reference": "MVVGLLGGSFDPPHPGHVHITREALKRFGLDRVWWLVSPGNPLKPRPPAPLARRLAEARRLMRHPRVAVTGLEAEIGTRFTAETLAVLQRRYPGVRFVWLMGADNLAQFHRWERWRAIMESVPVGVLARPGAGLRARTSPAARRYASALLPEAEAARLGRSAAPAWCFVNLPMMDLSSTEIRATGRWRGQAD",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must have high specificity towards nicotinate-nucleotide adenylyltransferase activity, ATP binding.2. A protein that is capable of catalyzing reactions within the deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1 pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "FLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITPIGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLYFAKLFDGLRHDSGDPYIWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKDRFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQVFDVPA",
    "response#2": "FKFADMSTRRRFSFQAQRTMLEYLSKELPQCLTGTSNYHLARELDLTPIGTVAHEWFMGHQALVNVRDSQKIALQRWQKMFNGALGIALTDTIGIDAFLKDFDEELSHAYVGVRHDSGCPFTWGEKMIAHYESLGIDPMTKTLVFTDGLNFEQALDICEHFQGRVQVSFGIGTSLANDMGNYVNDQGEAYQPLSIVIKMVTCNGSPVAKISDEPEKAMCEDIFFLMNLKRRFEQP",
    "response#3": "FLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITPIGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLYFAKLFDGLRHDSGDPYIWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKDRFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQVFDVPE"
  },
  {
    "reference": "MRLKGKNIILGITGSIAAYKAAMLLRLFIKEGAEVQVVITPAGKEFITPVTLSALSDKPVISEFFGANDGSWNSHVDLGLWADVMVIAPATASTMGKMAYGIADNMLITTYLSAKCPVMVAPAMDLDMFAHPSTQRNISILKDYGNSIVEPGEGELASGLTGKGRMEEPIKILEAVIQRLGVKKKLLNKSYLVTAGPTFEKIDPVRFIGNYSSGKMGYAIAEELAEQGADVTLVSGPVAVTTQKAGVKVIPVESANEMYNASIEHFERVDGAIMCAAVADFTPAKKEGEKTKRGKENWHIELEPTKDIAAELGKLKTNKQLLVGFALETNNEMSNAERKLLKKNLDFIVLNSLKDKGAGFGVDTNKITIIEKGNKQTDFELKDKAEVAKDIVAKIIELNN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein have enhanced H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.2. Ideal protein should have a binding affinity for key enzymes in the coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway to enhance the overall efficiency of the pathway.3. The designed protein should have phosphopantothenoylcysteine decarboxylase activity, phosphopantothenate--cysteine ligase activity to facilitate pantothenate catabolic process. The designed protein sequence is ",
    "response#1": "KNIXLGVTGGIAAYKAAEIVRHLKKSGSSVRVVMTKSAEEFITPLTLQALSGNRVSRELLDAEAEAAMGHIELAKWADGILIAPATANTLARLSSGRGDDLLSTXTLAFEGPISLAPAMNQAMWRDSRTQENLKKLIDKGYILCGPGSGEQACGDVGLGRMLEPLEIMGRRILLVIGGGIAAYKSLELIRRLRERGATVRAVMTPAACQFITPLSVGALTADRVFTDLFDREDEHDVGHIRLAREADLIVAAPATADLLAKMANGLANDLASTILLAAKVPVLVAPAMNPAMWQHPATRRNVAQLAGDGVAFVGPNSGAMAEAGEAGEGRMAEPLEIVAAIERRLSVASGPLAGKSVLITSGPTHEPIDPVRYIANRSSGKQGHAIAAAAARAGARVTLVSGPVTIPDPAGVRVLRVETAREMLQAVEAALPADVAIMAAAVADWRVASGSGQKIKKDGSGKPPALSLAENPDILATVGTLAEGRPCLVVGFAAETQNLIDNARAKLQRKGADFIVANDVSPETGVMGGDANTVRLVSRDGVEDWPTLSKEDVAERLVAVIAAKLADSGLEGLRVVVTAGGTREPLDPVRYVGNFSSGKMGRALAEVAAARGARVSLITTIPGASEEIDEVRVTTAQEMLEAVQRESAQADVLVMAAAVADYRPAEVASSKIRRTSKPLDLRLQPNVDILAAVGRRKGLFRVGFAAETSDVEPQAREKLKAKELDLIVANQVGVNGQGLGSDFNAVTILFQGESAAPIPYGPKSQLASRLFAVIAE",
    "response#2": "GRRILLVIGGGIAAYKSLELIRRLRERGATVRAVMTPAACQFITPLSVGALTADRVFTDLFDREDEHDVGHIRLAREADLIVAAPATADLLAKMANGLANDLASTILLAAKVPVLVAPAMNPAMWQHPATRRNVAQLAGDGVAFVGPNSGAMAEAGEAGEGRMAEPLEIVAAIERRLSVASGPLAGKSVLITSGPTHEPIDPVRYIANRSSGKQGHAIAAAAARAGARVTLVSGPVTIPDPAGVRVLRVETAREMLQAVEAALPADVAIMAAAVADWRVASGSGQKIKKDGSGKPPALSLAENPDILATVGTLAEGRPCLVVGFAAETQNLIDNARAKLQRKGADFIVANDVSPETGVMGGDANTVRLVSRDGVEDWPTLSKEDVAERLVAVIAAKLADSGS",
    "response#3": "KTILLGVTGGIAAYKVPLLIRDLKKKDAKVNVVMTENAKKFVTPLIISTLSDRLVESLWEGGIPHISLREEASLLCIIPADYNIIGKAASGVADDLLSTVISTFDKEILFFPSMNYRMYDNPIFKKNIKILKDFGHVIIEPDTGELACDEEGRGRLPDISRIMLEIEAELGSFLTGKKALVTSGPTHEPIDPVRYIANRSSGKQGHAIAAELAKLGADVTLVSGPSQQPDPTSVKVIKIESARDMLAACENSLPCDIAVFAAAVADWRVDLEAEQKLKKDGTGQIPDLKLTENPDILATISQHPDKRPTLVIGFAAETEHVIEHAQAKRERKKCDWIIANDVSPATNTFGGDENTVHLLFWPQGDEPLPLAPKEVLARQLIARIAQRLNQA"
  },
  {
    "reference": "MSTAKSTKKSYKAPEPGEEIVISGIAGRFPESKNVEELKNNLLNKVDMITDDDRRWKLDHPDIPQRTGKINDVGKFDALFFGVHFKQAHTMDPMCRMLLEHAYEAVVDAGINPKQLRGSRTGVFVGACFSESEKTWFYEKLQVNGFGITGCSRAMLANRISYWLGVTGPSYTLDSACSSSLVALEHAYRCLQDGQCDSAIVGGSNLCLHPYVSLQFARLGVLSPDGRSKAFDADADGYARSETISVIYLQKAKNAKRIYATVVYGKTNCDGYKEQGITFPSSLMQGNLLKECYEDCGLSPNNLSYLECHGTGTKVGDPEELNAIEKVICKERTAPLLIGSVKSNMGHPEPASGLCQVIKVIIAMETGIIPPNLHYNRPREGLKALEDGRLKVVTEQTPWDGGYVGINSFGFGGANCHVVLKSYSKKKINNGAPSDDLPRLVTVSGRTEDSIKVFLSDIERHPVDIEYIRLLHDIHSEDIQGHLYRGYTIVGSKKTEHIPKETENYLGTTRPIWFVFSGMGSQWPGMGIDLLRFPVFAEAVRKCDAVLRPRGVDIIDILTNKDKSTFDNILNSFVGIAAVQIGLVDLLTSVGIVPDNIIGHSVGELGCAYADGCFTAEQMILSAYSRGLASIETKVIFGSMAAVGLGYEDIKDMCPPDIEVACHNAADSSTISGPAESMKEFVAQLQAKQIFAKEVPCSNIPYHSRYIAPAGPKLLGYLSEVIPQPKPRSRKWVSSSVPRNNWSTPSAKFSSAEYHTNNLLSPVLFEET",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. We need a protein that can perform dodecanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxotetradecanoyl-   [ACP] + CO2 + holo-[ACP] with high efficiency.2. A protein that is capable of catalyzing reactions within the Lipid metabolism, fatty acid biosynthesis pathway with high efficiency and specificity.3. A protein with palmitoyl-[acyl-carrier-protein] hydrolase activity, oleoyl-[acyl-carrier-protein] hydrolase activity, enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity, 3-oxoacyl-[acyl-carrier-protein] synthase activity functions is required to enable fatty acid biosynthetic process.4. The protein contains novel Carrier domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MEEIVVAGISGRLPESDNLDEFWQNLFNGVDMVTEDDRRWKPGLYGLPRRNGKLKEIDRFDAAFFGVHPKQAHTMDPQLRLMLEISYEAIVDGGINPTSMRGTNTGVYIGVSGSEAGEAFSKDPEELLGYSMTGCQRAMFANRLSYFFDFNGPSTAIDTACSSSLLALENAFNAIRHGQCDAALVGGVNLLLKPNTSVQFMKLGMLSPDGACKSFDVSGNGYCRSEAAVAVLLTKKSMAKRVYATVLNAGNNTDGYKEQGVTFPSGDMQQRLVRSLYQEANISPEQVEYVEAHGTGTKVGDPQEVNGIVGVFCQSKREPLLIGSTKSNMGHPEPASGLAALAKVLLSLEHGMWAPNIHFNNPNPDIPALLDGRVRVVSEPIPVRGGIVGINSFGFGGSNVHIILRPAGKTCSPVPRTYDWLPNLQSAMRNETKTLVVVENQPLSGVLGFVNCLRREPGGTNVKCVFVQNGNIKFNLSDPLFREQLDKDLAINVLNQDVWGSYRHLPRWAERTGGMAFHSYFMDAIAPTLLQQLKKVIRDPQPRSSRWLSTSIPKTQWQESLARTFSAEYNVNNLVSPVLFQEALWQVPENAVVLEIAPHALLQAVLKRGLKTSCTIIPLMKKDHKDNLEFFLSSMGRLYLTGIDRWAERTGGMAFHSYFMDAIAPTLLQQLKKVIRDPQPRSSRWLSTSIPKTQWQESLARTFSAEYNVNNLVSPVLFQEALWQVPENAVVLEIAPHALLQAVLKRGLKTSCTIIPLMKKDHKDNLEFFLSSMGRLYLTGID",
    "response#2": "MEEIVVAGISGRLPESDNLDEFWQNLFNGVDMVTEDDRRWKPGLYGLPRRNGKLKEIDRFDAAFFGVHPKQAHTMDPQLRLMLEISYEAIVDGGINPTSMRGTNTGVYIGVSGSEAGEAFSKDPEELLGYSMTGCQRAMFANRLSYFFDFNGPSTAIDTACSSSLLALENAFNAIRHGQCDAALVGGVNLLLKPNTSVQFMKLGMLSPDGACKSFDVSGNGYCRSEAAVAVLLTKKSMAKRVYATVLNAGNNTDGYKEQGVTFPSGDMQQRLVRSLYQEANISPEQVEYVEAHGTGTKVGDPQEVNGIVGVFCQSKREPLLIGSTKSNMGHPEPASGLAALAKVLLSLEHGMWAPNIHFNNPNPDIPALLDGRVRVVSEPIPVRGGIVGINSFGFGGSNVHIILRPNAKLPKKLPYDWLPNLQSAMRNETKTLVVVENQPLSGVLGFVNCLRREPGGTNVKCVFVQNGNIKFNLSDPLFREQLDKDLAINVLNQDVWGSYRHLPRWAERTGGMAFHSYFMDAIAPTLLQQLKKVIRDPQPRSSRWLSTSIPKTQWQESLARTFSAEYNVNNLVSPVLFQEALWQVPENAVVLEIAPHALLQAVLKRGLKTSCTIIPLMKKDHKDNLEFFLSSMGRLYLTGIDYATVLHSKVNSDGFKSVGLFAPYWLRQKDLMVSTYEEAGVDPNDLVYFEAHGTGTNVGDPQEAKAIAEAYCKHREQPLLVGAVKSNLGHSEGSSGLNSVAKVIIAFENKCIPANLH",
    "response#3": "MEEIVVAGISGRLPESDNLDEFWQNLFNGVDMVTEDDRRWKPGLYGLPRRNGKLKEIDRFDAAFFGVHPKQAHTMDPQLRLMLEISYEAIVDGGINPTSMRGTNTGVYIGVSGSEAGEAFSKDPEELLGYSMTGCQRAMFANRLSYFFDFNGPSTAIDTACSSSLLALENAFNAIRHGQCDAALVGGVNLLLKPNTSVQFMKLGMLSPDGACKSFDVSGNGYCRSEAAVAVLLTKKSMAKRVYATVLNAGNNTDGYKEQGVTFPSGDMQQRLVRSLYQEANISPEQVEYVEAHGTGTKVGDPQEVNGIVGVFCQSKREPLLIGSTKSNMGHPEPASGLAALAKVLLSLEHGMWAPNIHFNNPNPDIPALLDGRVRVVSEPIPVRGGIVGINSFGFGGSNVHIILRPAEKPQVPTLGDSLPRLLHVSGRTEAAVQQLLDSAREHRDDQEFLSLIDDIHANNISGHSYRGYSILGTDENVKEVQTVANSRPVIFSGMGSQWAGMGKQLLVIETFERSLRRCAEALKPQGIDLLNLILNGTDETFESVLNSFVSIAAIQVALVDVLMMIDIQPDGIIGHSVGELGCAYADGTFTPEQTVLAAYWRGKSIEDSKLPPGAMAAIGLSWQETSKRCPPDISPACHNSADSVTVSGPTESIEKFVEQLKKDEIFAKVVNSSGVAFHSNYIASVGPKLRASLEKIISNPKQRSSRWISSSIPDTAWGTPLAQLSSPAYHVNLAVIRGSALNQDGASNGLTAPNGPAQERVIRQALADAGLQPSDVDAVEAHGTGTTLGDPIEANALINTYGRERPEEHPLWLGSLKSNIGHVQLAAGVAGVIKMVMAIRHAELPKT"
  },
  {
    "reference": "MENRWQVMIVWLVDRMRIRIWKSLVKHHMYISKKAKGWSYRHHYESTNPRISSEVHIPLGEXXAKLVITTYWGLHTGERDWHLGQGVSIEWKTKRYSTQVDPDLADQLIHLHYFDCFSESAIRKIIVGHRVSPRCEYQAGHNKVGSLQYLALAALVAPKXRKPPLPSVAKLTEDRWNKPQKTKGHRGSHTMNGH",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein localizes to the plasma membrane, host cell plasma membrane, host cell cytoplasm, cytoplasm.2. The protein must exhibit the following characteristics: Seems to play a role in viral morphology by affecting the stability of the viral nucleoprotein core; Finally, Vif also contributes to the G2 cell cycle arrest observed in HIV infected cells.; May interact directly with APOBEC3G mRNA in order to inhibit its translation; Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G; In turn, they are directed to the 26S proteasome for degradation; Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism; Recruits an active E3 ubiquitin ligase complex composed of elongin BC, CUL5, and RBX2 to induce polyubiquitination of APOBEC3G and APOBEC3F; Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions.3. Incorporating a BC-box-like motif motif is critical for the successful design of the protein. The designed protein sequence is ",
    "response#1": "MRVRGMQRNWPQWWIWGILGFWMVLICRGVGNEEEKGKIGNVTEPRNNSNDTKEMEKARERMGEDKRKYPIVRGIPIILGFLGAAGSTLLLGAITTSWEAPQREPTGPRRGPEGIEEEGGEQDRDRSIRLVNGFLALAWDDLRSLCLFSYHQLRDLILIAARTVGLLGRSSLRGLQRGWEAIKYLYHRLASILDYASNRLISAIGRVTRAVLHIPRRIRQGFERALL",
    "response#2": "MENRWQVMIVWQVDRMRINTWKSLVKHHMYVSKKAKDWFYRHHYESRHPKISSEVHIPLGDAKLVVTTYWGLHTGERDWHLGQGVSIEWRKKRYSTQVDPDLADQLIHLHYFDCFSDSAIRKAILGQIVRPRCEYQAGHNKVGSLQYLALTALIKPKKIKPPLPSVKKLTEDRWNKPQRTKGHRGNHTMNGH",
    "response#3": "MENRWQVMIVWQVDRMRIRAWNSLVKYHMYVSKKANNWYYRHHYESRHPRVSSEVHIPLGDAKLVVTTYWGLHTGEREWHLGQGVSIEWRKERYSTQVDPDLADQLIHLYYFDCFSESAIRNAILGHRVSPRCEYQAGHNKVGSLQYLALTALITPKKIKPPLPSVRKLTEDRWNKPQKTKGHRGNHTMNGH"
  },
  {
    "reference": "MVRILSELPRREEYSPLKNRRLLIGLTASSSIYRSIDLIRKLIRMGAKIKVVMTRESLSLITPDLVEWATGPKPLIEFTGRAEHIELTNWAEAFIIVPATLNTIAKIAYGIGDQLLHLTAITMMGAGKKLAILPTMNMKLYNSPQYREALNKLASYSNVSIINPLIDEGKAKFPPLDDVAHCIDALVNRGRDLEGKHILVTAGPTIEYIDPVRIITNNSSGLMGVLLAREAVCRGATVDLVHGPLRIDPPYNVNKYPVTTTAQMAKTISKLTNEKQYDAAIFAAAPADYTVLSRSRKKISTRELGSLVIRLKQTPKTVKFVSRNNRPKLMIIFSAETIDNHLELVEKARNKLVDYNADLAIANNVSISGIGFSSSYIDACIVSRDSYECMGVIRKEVLMRKIIDLIKQNI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis pathway to avoid interference with native metabolic processes.2. The protein must bind to Mg(2+) in order to perform its enzymatic function.3. Incorporate a signal peptide in the protein design.4. The designed protein must possess phosphopantothenoylcysteine decarboxylase activity, FMN binding to support coenzyme A biosynthetic process, pantothenate catabolic process under certain conditions.5. For general function, the protein need meet that In the first step cysteine is conjugated to 4'- phosphopantothenate to form 4-phosphopantothenoylcysteine; In the second step the latter compound is decarboxylated to form 4'- phosphopantotheine.6. The protein contains novel Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains that confer a unique function or activity.7. The protein should have a low Km value for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2. The designed protein sequence is ",
    "response#1": "MRIVLGVGGGIAAYKVASLLRLFTEAGHQVTVIPTESALRFVGVATWEALSGNPVSNSVFDDVEKVNHVRLGHEADLIVIAPATADLLAKAATGQAGDLLTNTLLMAHGPVLFAPAMHTEMWQHAATQANVATLRSRGVTVLDPAVGRLTGADSGPGRLPEPDVIAALAAALDGSGGLTGRKVLITAGGTREPLDPVRFIANRSSGKMGYALATEAVRRGGQVVLVSGPVELAPPPGLAEFIRVERAEEMAEAVLSRAPEMDVIIKAAAVADFRPVQVAEQKVKKGDGDWVLHLQKNPDILAILGARKRPGQVLVGFAAETHDLLANGAEKARRKGADFIVANDVSRPGSGFGSDTNEVTFLASGPDPVTELRGSKLQVADAVVARIAAMMDEPVTA",
    "response#2": "MRIVLGVGGGIAAYKVASLLRLFTEAGHQVTVIPTESALRFVGVATWEALSGNPVSNSVFDDVEKVNHVRLGHEADLIVIAPATADLLAKAATGQAGDLLTNTLLMAHGPVLFAPAMHTEMWQHAATQANVATLRSRGVTVLDPAVGRLTGADSGPGRLPEPDVALAARIALGPDLTGRKVLITAGGTREPLDPVRFIANRSSGKMGYALATEAVRRGGQVVLVSGPVELAPPPGLAEFIRVERAEEMAEAVLSRAPEMDVIIKAAAVADFRPVQVAEQKVKKGDGDWVLHLQKNPDILAILGARKRPGQVLVGFAAETHDLLANGAEKARRKGADFIVANDVSRPGSGFGSDTNEVTFLFDDGGARELDRCPKRVLADRIWDLVARRL",
    "response#3": "MRIVLGVGGGIAAYKVASLLRLFTEAGHQVTVIPTESALRFVGVATWEALSGNPVSNSVFDDVEKVNHVRLGHEADLIVIAPATADLLAKAATGQAGDLLTNTLLMAHGPVLFAPAMHTEMWQHAATQANVATLRSRGVTVLDPAVGRLTGADSGPGRLPEPDVEAAAELLLAPGLTGRRIVVTSGPTHEPIDPVRYIANRSSGAQGTAIARALAGLGAEVVFVTGPARVPPPAGVQVVRVETAREMLEAVQAALPAEAAVFAAAVADWRVANAAGQKVKKDGSGKAPALEFAENTDILATVAQMGEGRPVLVIGFAAETHDVEAHARAKRARKACDWIVANDVSPGTGIMGGAENEVTLLWADGTEELEGQTVMVTAGPTREEIDPVRFISNHSSGRMGYAVARAARQRGARVILVSGPTGLAAPAGVERICVESARDMQAAVMEHVKECAVVVKTAAVADYRPAERKGDKIKKQAAEMTLALVKNPDILAELGRLKKRPFLVGFAAETSRLEEYAARKLAEKNLDMIVANDVSLGDAGFNVDTNRALLLGADGSETPVPHGSKTALAAAIVDAVAARLPHHP"
  },
  {
    "reference": "MRVKGILRNCQQWWIWGILGFWMVMXCKGVEXEEKXWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQEMIMENVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLECKNVTXNATXNGNDTXNEMKNCSFNTTTEIRDRXRXNYALFYRLDIVPISDGKGNYRNSSEYXLINCNTSTLTQACPKISFDPIPIHYCTPAGYAILKCNDKXFNGTGPCXNVSTVQCTHGIKPVVSTQLLLNGSLAEXXIVIRSANLTDNAKTIIVQLNESVEIVCTRPGNNTRKSIRIGXGXXXYXNNXIIGDIRQAHCNIXXTXWDKTLQXVVXKLKEHFPNKTIXFNQSSGGDLEITTHSFNCGGEFFYCNTSQLFNSTHMFNXTYNXTQGNSXSNITLQCRIKQIINMWQTVGRAMYAPPIAGNITCKSXITGLLLQRDGGGNTNETEIFRPGGGDMRDNWRSELYKYKVVEIKPLGIAPTKAKRRVVEREKRAVGIGAVLLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGIWGCSGKLICTTTVPWNSSWSNKSLESIWXNMTWMQWDREINNYTNTIYQLLEESQNQQEQNEKDLLALDKWXNLWTWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSLQTLTPSPRGPDRLGRIEEEGGEQDXDRSIRLVSGFLALAWDDLRSLCLFXYH",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must have at least one Human immunodeficiency virus 1 envelope glycoprotein Gp120 domain.2. The protein must contain a signal peptide for proper functionality.3. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.4. The protein's functional design necessitates the inclusion of a YXXL motif; contains endocytosis signal motif.5. The designed protein should have at least Helical transmembrane domains to ensure proper localization.6. A protein that can perform structural molecule activity in virion membrane, host cell endosome membrane, plasma membrane, host cell plasma membrane, viral envelope to support clathrin-dependent endocytosis of virus by host cell, mitigation of host antiviral defense response, positive regulation of receptor clustering, fusion of virus membrane with host endosome membrane, virion attachment to host cell. The designed protein sequence is ",
    "response#1": "MWVTVYYGVPVWKEATTTLFCASDAKSYEKEAHNIWATHACVPTDPNPQEIELENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDFVKNTTRVNMTSGNTTINTPSTLINVTMNATDTDMKNCSFNITTVLRDKQKKVYSLFYKLDVVQIGENNNSYRLINCNTSAITQACPKITFEPIPIHYCAPAGFAILKCNDRKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIIIRSENLTNNAKIIIVQLNESVTINCTRPNNNTRTGIPIGPGRAFYATGDIVGDIRQAYCTISREQWNKTLQRVATQFRVLLNKTTIVFNSSSGGDAEITTHSFNCGGEFFYCNTSNLFKGTFNGTSNSTGNNENQNITLPCRIKQIINMWQEVGKAMYAPPIAGIIKCSSNITGLLLTRDGGTENNDSETIRPGGGDMRDNWRSELYKYKVVRLEPLGLAPTRAKRRVVEREKRXLGAGAIGFLGFLGAAGSTMGAASMTLTVQARLLLSGIVQQQNNLLRAIEAQQRMLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNTSWSNKSLNEIWNNMTWMEWEREIDNYTSLIYTLIEESQNQQEKNEQELLELDKWASLWNWFDITNWLWYIKIFIMIVGGLVGLRIVFTVLSIVNRVRQGYSPLSFQTRLPAQRGPDRPEGIEEEGGERDRDRSRPLVDGFLTLIWVDLRSLCLFSYHRLRDLLL",
    "response#2": "MWVTVYYGVPVWKEATTTLFCASDAKSYEKEAHNIWATHACVPTDPNPQEIELENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDFVKNTTRVNMTSGNTTINTPSTLINVTMNATDTDMKNCSFNITTVLRDKQKKVYSLFYKLDVVQIGENNNSYRLINCNTSAITQACPKITFEPIPIHYCAPAGFAILKCNDRKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIIIRSENLTNNAKIIIVQLNESVTINCTRPNNNTRTGIPIGPGRAFYATGDIVGDIRQAYCTISREQWNKTLQRVATQFRVLLNKTTIVFNSSSGGDAEITTHSFNCGGEFFYCNTSNLFKGTFNGTSNSTGNNENQNITLPCRIKQIINMWQEVGKAMYAPPIAGIIKCSSNITGLLLTRDGGTENNDSETIRPGGGDMRDNWRSELYKYKVVRLEPLGLAPTRAKRRVVEREKRXLGGLGALFLGFLGAAGSTMGAASLTLTVQARLLLSGIVQQQNNLLRAIEAQQRMLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNTSWSNKSLNEIWNNMTWMEWEREIDNYTSLIYNLIEESQNQQEKNEQELLELDKWASLWNWFDITNWLWYIKIFIMIVGGLVGLRIVFTVLSIVNRVRQGYSPLSFQTRLPAQRGPDRPEGIEEEGGERDRDRSGRLVDGFLTLIWVDLRSLCLFSYHRLRDLLLIVT",
    "response#3": "MWVTVYYGVPVWKEATTTLFCASDAKSYEKEAHNIWATHACVPTDPNPQEIELENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDFVKNTTRVNMTSGNTTINTPSTLINVTMNATDTDMKNCSFNITTVLRDKQKKVYSLFYKLDVVQIGENNNSYRLINCNTSAITQACPKITFEPIPIHYCAPAGFAILKCNDRKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIIIRSENLTNNAKIIIVQLNESVTINCTRPNNNTRTGIPIGPGRAFYATGDIVGDIRQAYCTISREQWNKTLQRVATQFRVLLNKTTIVFNSSSGGDAEITTHSFNCGGEFFYCNTSNLFKGTFNGTSNSTGNNENQNITLPCRIKQIINMWQEVGKAMYAPPIAGIIKCSSNITGLLLTRDGGTENNDSETIRPGGGDMRDNWRSELYKYKVVRLEPLGLAPTRAKRRVVEREKRXHLGXYPIWDXI"
  },
  {
    "reference": "MLSHIVEYECQYTDQLYKKRKIWHDGRLKYFQLNNRFMLYTEKDNVLLASEFKINSKELKAILNPEGFDIEEHRIFSQFLVIISNIIEEYDRDIQVAATHVRAYPSNLSVQKQRPLISDNAPSLNHISTAREVHSNIKVTTPNRKQTEDNATKGGFNISKLTLKVNKPFKKPKRILSTNVVNESNRPSIRSQKIQEVTPQLHETNTSTKVQTAGKVALNNDNIAQGNYATITEEAKVRDGSDRKKDMANLSKSGKRRVGGIRRIVHEPLGI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be designed to localize specifically to nucleus, cytoplasm, site of double-strand break, chromosome, telomeric region in order to perform its role in negative regulation of telomere maintenance, double-strand break repair, positive regulation of helicase activity more effectively. The designed protein sequence is ",
    "response#1": "NPVQDFEAMLTQRSSSTWVQTAIEEMQKYITALIQDSCDRDNHQKALECLVALRKACIIEQEPNEYNGFVTKLCQKFRPAGDKIFLQLLSSKKASLISKEEAPDSDVTEEMARNFL",
    "response#2": "YPVMEKSLEFWRARDFARKLEVLKPLFVEPRSKGGFSEVWSLGSTALLGWPLSLTRGSGQLQAVWHLVPAGTASPQRRIPAALLVEVEGPRAGARLTSQSFQVMDAYYARVAAPGSSGATFLAVCRGKASEGLDFADVNGRGVIVTGLPYPPRMDPRVVLKMQFS",
    "response#3": "NPTMKIKVWVYKKVAEERLPTLKMYSDKAPPTDKFAKHEVKVDYDYKVTAESTEVIAPEERIKGFRYGPQVIPISPDQIETLKFKTDKGMKLLGFTEASNILRHYYMKDVNIVVPDPSKEKSVLAVSAIAREMKETNKVAIVRCVWRNGQGNVVVGVLTPNVSERDDTPDSFYFNVLPFAEDVREFPFPSFNKLPSSWKPDEQQC"
  },
  {
    "reference": "MNNLLDRFLNYVSFDTQSKPGVRQVPSTEGQFRLARALQSELLALGLEQVTLSEHGCVMATLPANVAWPVPTIGFIAHMDTAPDASGKNVNPQIVENYRGGDIALGIGDEILSPVMFPVLHQLLGQTLITTDGKTLLGADDKSGIAEIMTAMVRLKQGNTPHGEIRVAFTPDEEVGKGAQHFDVAAFGAEWAYTVDGGGVGELECENFNAASVNIKIIGNNVHPGSAKGVMVNALGLANRIHALLPAAEVPEQTDGYEGFYHLVSMKGSVEKAEMHYIVRDFSREGFEARKKHMMAIAKQVGQGLHPDCYIEVTLDDSYYNMRDEVAKHPHIVALARQAMCDLAIEPIERPIRGGTDGAQLSFHGLPCPNLFTGGYNFHGKHEFITLEGMEKAVSVIMRIAELTAGRARVAG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. We need a protein that can perform Release of the N-terminal residue from a tripeptide. with high efficiency.2. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding.3. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "MMAWMNAESVAKTLETGRVTYWSRSRQSFWIKGESSGHVQELVELRVDCDRDALLAMVRQTGPACHTNRRSCFD",
    "response#2": "TSVLTRPDYYDGLKPVAANIDQMVIVSSVLPELSLNIIDRYLIAAETLGIEPLLVLNKIDLLQEAELATYREWLADYEKIGYKILYVSKRSGAGIAELEAQLQDRINIFVGQSGVGKSSLVNALIPELDIEEGEISELSGLGQHTTTAARLYHIPSGGNLIDSPGVRETSVLTRPDYYDGLKPVAANIDQMVIVSSVLPELSLNIIDRYLIAAETLGIEPLLVLNKIDLLQEAELATYREWLADYEKIGYKILYVSKRSGAGIAELEAQLQDRINIFVGQSGVGKSSLVNALIPELDIEEGEISELSGLGQHTTTAARLYHIPSGGNLIDSPGVRETSVLTRPDYYDGLKPVAANIDQMVIVSSVLPELSLNIIDRYLIAAETLGIEPLLVLNKIDLLQEAELATYREWLADYEKIGYKILYVSKRSGAGIAELEAQLQDRINIFVGQSGVGKSSLVNALIPELDIEEGEISELSGLGQHTTTAARLYHIPSGGNLIDSPGVRETSVLTRPDYYDGLKPVAANIDQMVIVSSVLPELSLNIIDRYLIAAETLGIEPLLVLNKIDLLQEAELATYREWLADYEKIGYKILYVSKRSGAGIAELEAQLQDRINIFVGQSGVGKSSLVNALIPELDIEEGEISELSGLGQHTTTAARLYHIPSGGNLIDSPGVREGANIDQMVIVSSVLPELSLNIIDRYLIAAETLGIEPLLVLNKIDLLQEAELATYREWLADYEKIGYKILYVSKRSGAGIAELEAQLQDRINIFVGQSGVGKSSLVNALIPELDIEEGEISELSGLGQHTTTAARLYHIPSGGNLIDSPGVGTSVLTRPDYYDGLKPVAANIDQMVIVSSVLPELSLNIIDRYLIAAETLGIEPLLVLNKIDLLQEAELATYREWLADYEKIGYKILYVSKRSGAGIAELEAQLQDRINIFVGQSGVGKSSLVNALIPELDIEEGEISELSGLGQHTTTAARLYHIPSGGNLIDSPGVRE",
    "response#3": "MMAWMNAESVAKTLETGRVTYWSRSRQSFWIKGESSGHVQELVELRVDCDRDALLAMVRQTGPACHTNRRSCFK"
  },
  {
    "reference": "MGYFQGNDFRKITGGKKGKHRDKRKFELGSPPTETKLSTEELIEKERGMGGNIKIRVKYATFANIYDPQEKVSKKAKILSVIETPANKEYARRGIIVKGSIIQTELGKAKVTSRPGQDGTINAILIKE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein localizes to the ribosome. The designed protein sequence is ",
    "response#1": "LVGVGYKATATNNILELSLGYSHGIFLKLPSEISASALTEKGKNPIVTLECIDKQLIGQVSAKIRSLRKIEPYKGKGVRV",
    "response#2": "MRAHTDVVTAIATPIDNSDMIVTASRDRSIILWHLTKEDKTYS",
    "response#3": "MRAHTDVVTAIATPIDNSDMIVTASRDRSIILWHLTKEDKTYY"
  },
  {
    "reference": "MSTGFFGDIAKIKYEGPDSTNPLAFRHYNPDEIVGGKRMEDHLRFAVAYWHTFTWPGGDPFGGQTFQRPWFEDTMQAAKLKADVAFEFFSLLGSPFYCFHDADVRPEGKNFAENTKNLNEIVDYFAQKQADTGVKLLWGTANLFSNRRFMSGAATNPDPDVFAFSAATVKTCMDATKTLGGANYVLWGGREGYETLLNTDLSRELDQLGRFLNLVVEYKYKIGFEGTILIEPKPQEPTKHQYDYDVATVYAFLQKNGLEKEVKVNIEQGHAILAGHSFEHELAMANAFGIFGSIDMNRNDYQSGWDTDQFPNNVPEMALAYYHVLAGGGFKNGGTNFDSKLRRQSLDPQDLLIGHIGGMDCCARGLKAAAKMIEDGALSKPLSERYAKWDSPEAQKMLRGELKLEEIAALVERDDINPEPKPGRQEYLENVVNRYV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The ideal protein would be one that plays a critical role in D-xylose metabolic process through magnesium ion binding within cytoplasm.2. The protein should be able to bind Mg(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "HKLGVDYLCFHDRDLAPEGDTLRETNRNLDKVIDKIVDYQKQTGMKVLWNTSNMFTNPRFVAGAATSPDADVFAYAAAQLKHSLEIGKRVGAENYVFWGGREGYESLWNTNMKLEQEHAAKFFHMAKDYANEIGFDAQMLLEPKPKEPSTHQYDFDAATTIAFMKEYDLDKDFKLNLEGNHANLAGHTYQHEIRVAA",
    "response#2": "RHVELGYRAVRVQSGVPGIETTYGVAKTPGERYEPADSSLPAEHVWSTEKYLNHAPKLFAAVRERFGDDLHVLHDVHHRLTPIEAARLGKAVEPYHLFWLEDCVPAENQESLRLIREHTTTPLAIGEVFNSIHDCRELIQNQWIDYIRMPLTHGGGITAMRRVADLASLYHVRTGFHGPTDLSPVCLGAAIHFDTWVPNFGIQEHMPHTDETDAVFPHDYRFEDGHFLAGESPGHGVDIDEL",
    "response#3": "KEKGKLNATGATQKGWSQTHRNFINELFNEGYRLRYSGAMVSDLHQILLKGGGLFSYPATSDHPNGKLRVVFEVLPFAFIYENAKGATTDGKNQTLFDIKIEKIHQTTPCFFGSRDEISLLHKFK"
  },
  {
    "reference": "SFCIPFKPCKSDENCCKKFKCKTTGIVKLCRW",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be expressed specifically in the extracellular region The designed protein sequence is ",
    "response#1": "GKWYGIGLASNSNWFQSKKQQLKMCTTVITPTADGNLDVVATFPKLDRCEKKSMTYIKTEQPGRFLSKSPRYGSDHVIRVVESNYDEYTLMHTIKTKGNEVNTIVSLFGRRKTLSPELLDKFQQFAKEQGLTDDNILILPQTC",
    "response#2": "LMQFETLIMKIAGRSGVWWYGSYGCYCGAGGQGRPQDPSDRCCFVHDCCYGKVTGCNTKDEFYTYSEENGAITCGGENPCLKEVCECDLAAAICFRDNLDTYNSKKYWMFPAKNC",
    "response#3": "LMQFETLIMKIAGRSGVWWYGSYGCYCGAGGQGRPQDPSDRCCFVHDCCYGKVTGCNTKDEFYTYSEENGAITCGGENPCLKEVCECDLAAAICFRDNLDTYNSC"
  },
  {
    "reference": "MIDWLYSYPPLYPGILLKRYKRFFADVQLASGEVVTAHCPNTGPMTGVSTLGSVVQLSKSANPKRKLAYTLELIQVHDNEPTWVGVNTALPNQIVKLALAKYLFPELGSYNHIKSEVVYGVDKKSRVDFFLTGSDTERPIYLEVKNTTWAKGTLALFPDTETTRGQKHLRELMTLLPQTRSVMLYFINRGDCTEFAPGDSTDPIYGKLLREAIALGLEVLPCRFDVTPEGIRYLGLAKLVI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must be able to perform DNA binding under specific conditions. The designed protein sequence is ",
    "response#1": "LIEVQRSSYDHFLQMDVPPEKRANVGLQEVFRSVFPIKDFSERAVLDFVRYELEQPKYDVEECQQRGMTFAAPLKVTLRLTVFDVDEDTGLRSIRDIKEQDVYMGDMPLMTANGTFIINGTERVIVSQMHRSPGVFFDHDKGKTHSSGKYLFAARVIPYRGSWLDFEFDAKDIVYVRIDRRRKLPATTLLYALDGADSAELRAERRALGKDLLPYEAQGMAKEEILGYFYETITYQRAADGWKTGFDAERMKGQKLLTDLVDARTGEVLASRDTKLTPRLIRKLQDQGLQEIKVAIEDIIGRYLAIDIIDEKTGEVIYEAGDELSATALERLEKMGVEELPVLNVDHLNIGAYIRNTMAADRNASREDALIDIYRVMRPGEPPTLESAEALFAGLFFDQERYDLSAVGRVKMNARLGFETDDQMRVLRKEDILKILKILVELKDGRGEIDDIDHLGNRRVRSVGELMENQYRVGLLRMERAIRERMSSVEIDTVMPHDLINAKW",
    "response#2": "HRRIAEWAIANKVCSCFENVLEVIPEQKFGDSRLDFLLKKSDTELWVEVKGCTLIYGNTATFPDAPTTRGKRHVGELKKALESGSEALILIIILRKDALCFKANASIDPDFAEVFKDAVNAGVQVCPLVFG",
    "response#3": "HRRIAEWAIANKVCSCFENVLEVIPEQKFGDSRLDFLLKKSDTELWVEVKGCTLIYGNTATFPDAPTTRGKRHVGELKKALESGSEALILIIILRKDALCFKANASIDPDFAEVFKDAVNAGVQVCPLVFE"
  },
  {
    "reference": "MSSIYGLLNFMSATELLLAITVFCLGFWVVRALRTQVPKGLKTPPGPWGLPILGHVLTLGKNPHLSLTKLSKQYGDVLQIRIGSTPVVVLSGLDTIRQALVRQGDDFKGRPDFYSFTLITNGKSMTFNPDCGPVWAARRRLAQDALKSFSIALDPASASSCYLEEYVIKEADYLISKFQKLMAEVGHFDPDRYLVVSVTNVICAMCFGQRYDHDDQELLSIVNLSNEFGKVTGSGYPPDFIPILRYLPNSSLDAFKDLNKKFYSFMQKSVKEHYRTFEKGHIRDITDSLIEHCQDKSLDENANVQLSDDRVINIIVDLFGAGFDTVTTAISWSLMYLVTNPGVQRKIQEELDTVIGRSRRPRLCDRSQLPYLEAFILETFRHSSFLPFTIPHSTTRDTSLCGFYIPKGHCVFVNQWQINHNQELWGDPNKFRPERFLTSSGTLDKVLSGKVTLFGLGKRKCIGETIGRLEVFLFLAILLQQIEFTVSPGEKVDMTPIYGLTLKHARCEYFQAQTRSSGPQHPQA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that can modulate the overall flux of metabolites through the retinol metabolism pathway, enhancing or suppressing its activity as needed.2. The protein should have a low Km value for (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate = 5-oxo-   (6E,8Z,11Z,14Z)-eicosatetraenoate + H2O.3. The protein must be engineered to have a high affinity for heme in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "DSFNRIFWCGQSKLAERVRDSWKEDALFGYQFLNGTNPMLLRHSVELPARLKFPPGMEELQAQLEKELQGGTLFEADFSLLDGIKANVILCSQQYLAVPLVMLKLQPDGKLLPMVIQLQLPHEGSPLPPLFLPTDPPMVWLLAKCWVRSSDFQLHELHSHLLRGHLMAEVIAVATMRCLPSIHPIFKLLIPHFRYTMEINVRARNGLVSDLGIFDQVVSTGGGGHVELLRRAAALLTYSSFCPPDDLADRGLLGVESSFYAQDALRLWEVISRYVEGIVSLHYKTDESVKEDLELQAWCREFTEIGLLGAQDRGFPVSLQSKEQLCHFVTMCIFTCTGQHSSNHLGQLDWYSWVPNAPCTMRLPPPTTKDATLETVMATLPNFHQASLQMSITWQLGRCQPTMVALGQHEEEYFSGPGPKAVLTKFREELAALDKDIEVRNAKKK",
    "response#2": "RTVVDDPQGLFKKHREEELAERRKLYRWGNWKDGLILNIASTGIHDLPVDERFLEDKRIDFEASLAKGLADLAVKDSLNVLMSWNSLDSFNRIFWCGQSKLAERVRDSWKEDALFGYQFLNGTNPMLLRHSVELPARLKFPPGMEELQAQLEKELQGGTLFEADFSLLDGIKANVILCSQQYLAVPLVMLKLQPDGKLLPMVIQLQLPHEGSPLPPLFLPTDPPMVWLLAKCWVRSSDFQLHELHSHLLRGHLMAEVIAVATMRCLPSIHPIFKLLIPHFRYTMEINVRARNGLVSDLGIFDQVVSTGGGGHVELLRRAAALLTYSSFCPPDDLADRGLLGVESSFYAQDALRLWEVISRYVEGIVSLHYKTDESVKEDLELQAWCREFTEIGLLGAQDRGFPVSLQSKEQLCHFVTMCIFTCTGQHSSNHLGQLDWYSWVPNAPCTMRLPPPTTKDATLETVMATLPNFHQASLQMSITWQLGRCQPTMVALGQHEEEYFSGPGPKAVLTKFREELAALDKDIEVRNAKLALPYEYLRPSRVENSVAV",
    "response#3": "RTVVDDPQGLFKKHREEELAERRKLYRWGNWKDGLILNIASTGIHDLPVDERFLEDKRIDFEASLAKGLADLAVKDSLNVLMSWNSLDSFNRIFWCGQSKLAERVRDSWKEDALFGYQFLNGTNPMLLRHSVELPARLKFPPGMEELQAQLEKELQGGTLFEADFSLLDGIKANVILCSQQYLAVPLVMLKLQPDGKLLPMVIQLQLPHEGSPLPPLFLPTDPPMVWLLAKCWVRSSDFQLHELHSHLLRGHLMAEVIAVATMRCLPSIHPIFKLLIPHFRYTMEINVRARNGLVSDLGIFDQVVSTGGGGHVELLRRAAALLTYSSFCPPDDLADRGLLGVESSFYAQDALRLWEVISRYVEGIVSLHYKTDESVKEDLELQAWCREFTEIGLLGAQDRGFPVSLQSKEQLCHFVTMCIFTCTGQHSSNHLGQLDWYSWVPNAPCTMRLPPPTTKDATLETVMATLPNFHQASLQMSITWQLGRCQPTMVALGQHEEEYFSGPGPKAVLTKFREELAALDKDIEVRNAKLALPYEYLRPSRVENSVAI"
  },
  {
    "reference": "MSTSLDLWNIPLRALNINVRKRLGLFLNPRNTVASDWMSVAENMGFSYLEIKNYEDCLDPTRRILEDWQARCPGAKVGKLLSILDNVDRKDVVEDLRDLIEEDCRRYIERQNEPPLQVPEVDSCVPKTQERQGITLEDDPEGGIPELFDAFICYCQSDFDFVHEMXQQLEQTDHKLKLCVFDRDVLPGSCVWTITSELIEKRCKRMVVVISDEYLDSDACDFQTKFALSLCPGARSKRLIPVKYRSMKKPFPSILRFLTVCDYTRPCTQSWFWVRLARALSLP",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response..2. The protein should be optimized for stability and solubility when expressed in microtubule organizing center where it will be involved in negative regulation of gene expression. The designed protein sequence is ",
    "response#1": "SSDWQMKEKGLVSIQRLAACHSEVLTGKLHDVCLVVTGEVTNLRSKVSHLAISTLGDLFQALKKNMDQEAEEIARCLLQKMADTNEFIQRAAGQSLRAMVENVTLARSLVVLTSAGVYHRNPLIRKYAAEHLSAVLEQIGAEKLLSGTRDSTDMLVHNLVRLAQDSNQDTRFYGRKMVNIL",
    "response#2": "EIVGELGDGAFGKVYKAKNKETGALAAAKVIETKSEEELEDYIVEIEILATCDHPYIVKLLGAYYYDGKLWIMIEFCPGGAVDAIMLELDRGLTEPQIQVVCRQMLEALNFLHGKRIIHRDLKAGNVLMTLEGDIRLADFGVSAKNLKTLQKRDSFIGTPYWMAPEVVLCETMKDAPYDYKADIWSLGITLIEMAQIEPPHHELNPMRVLLKIAKSDPPTLLTPSKWSVEFRDFLKIALDKNPETRPSAAQLLQHPFMLIYNDIISGDELISDSYDLVEVDGVAYEADCRKITIGGETFDTGANASAEGGDEEADDQKETKIDVVHAFQLQETNFDKKAYLGHLKSYMKKIKESMAASGASEDEVKEFEKGAQTFAKRVVGSFKDYEFLIGPSMDPDAMVVLLNYREDGVTPYVTLWKHGLKSTKL",
    "response#3": "VCVCHSEEDLVAAQELVAYLEGSTASLRCFLQLRDATPGGAIVSELCQALSSSHCRVLLITPGFLRDPWCKYQMLQALTEAPGAEGCTIPLLSGLPRAACPAELRFMYFVDASRGPDRGFHC"
  },
  {
    "reference": "MANPRHRHTPSRRDKRRANWKATAPNLALCPECKEPKLPHRVCPNCGTYKGRKILEVEE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be optimized for structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "LKVVADIPVQVIGEPHPDTLVDQQVTTIAVKADATRLPGPIEVDITGLEPGTSILARQLVLPAGSELDADPDLVVVQCLAK",
    "response#2": "MEKVVADIPVQVIGEPHPDTLVDQQVTTIAVKADATRLPGPIEVDITGLEPGTSILARQLVLPAGSELDADPDLVVVQCLAK",
    "response#3": "MPKVVADIPVQVIGEPHPDTLVDQQVTTIAVKADATRLPGPIEVDITGLEPGTSILARQLVLPAGSELDADPDLVVVQCLAK"
  },
  {
    "reference": "MSATQYYGTGRRKTSTARVFLKAGSGNLVINNRSIDQYFGRETARMVVRQPLELVDATEKFDVYITVKGGGISGQAGAIRHGITRALMQYDETLRRTLRSAGFVTRDSREVERKKVGLRKARRRPQFSKR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have structural constituent of ribosome and be active in ribosome, ribonucleoprotein complex. The designed protein sequence is ",
    "response#1": "GVASPRI",
    "response#2": "MMKIFH",
    "response#3": "GYFS"
  },
  {
    "reference": "MRGLGLWLLGALGLPAMAPGRPWAPLEQYEVVLPRRLPGPRARRALPSHSGLYPERVSYVLAATGYSFTLHLRKNRDLLGSGYTETYTAANGSEVTEQPRGQDLCFYQGHVEGHEDSAASISTCLGLRGFFQVGSDLHLIEPLDEGGEGGRHAVYPAEQLLQTAGTCGVSNDTLHRLLGPRTAAVFRSRPGAVDFTGDTVGFAKVSAMCSHSSGAVNQDHSKNPVGVACTMAHEMGHNLGMDHDEQVHGCRCREPSEGMHCVMAGRISSKFPRTFSDCSRAYLESFLEQPQSTCLANAPDLSLLVGGPVCGNLFVERGEQCDCGPPEECRNRCCNSTTCQLVQGAQCAHGTCCQECRVKPAGELCRPQKDTCDLEEFCDGRHPECPEDAFQENGTPCFGGYCYNGTCPTLAQRCQALWGPGGHAAKESCFFYDISPNCRSSRSRADMCGVLQCQGGQHPPGRGSCTLNDICRALTTEGGTAYEPVPEGTRCGPEKVCWKGRCQDLHVYRSRNCSAQCHNHGVCNHKQECHCHAGWAPPHCAELLTEAHTASESLPVVLVVVLALLAVVVVTLVGGVIYRKARSRVQSRSAAPKTTMGRSNPLFHQASSSALAKGGAPAPTRVPHEQVPTTHPGQPARHAASSMAPKRPPPAPPAAVSSTPFPVPVYTQQAPKQVVRPTFTPPVPPVKPGAGTAKPGAAEGAVGPKVALKPPIQRKPGAGAPTMP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design must be focused on enhancing the efficiency of angiogenesis, positive regulation of NF-kappaB transcription factor activity. The designed protein sequence is ",
    "response#1": "PVAHVVADPAAQGQLQWEKRFANTLLANGVKLEDNQLVVPTDGLYLIYSQVLFSGQRCPSTPVFLTHTISRLAVSYPNKANLLSAIKSPCQGGTSEEAEAKPWYEPIYLGGVFQLEKDDRLSAEINMPNYLDFAESGQVYFGIQ",
    "response#2": "NVPSIQEYSASLSTYNDQSITFVFEDGSYEIYVEDLRKGQEKDKVLFRYYDSQSPSHETGDDVDGQTLLVNLSPTKDKDFLLHANNEEHSVELQKCENQLPDQAFFLLHRKSSECVSFECKNNPGVFIGVKDNHLALIKVGDQTKDSYIEKTIFKLK",
    "response#3": "LQWEKRFANTLLANGVKLEDNQLVVPTDGLYLIYSQVLFSGQRCPSTPVFLTHTISRLAVSYPNKANLLSAIKSPCQGGTSEEAEAKPWYEPIYLGGVFQLEKDDRLSAEINMPNYLDFAESGQVYFGIIAR"
  },
  {
    "reference": "MDGQAEGSSQYRRVHLPPRSEYRFELEPHERLSIRLVQGRTQSGEDPDAEIFGAELVGGSQERWYPFGDEAKAAVSSWRGAEIEVAGSASTEYLAEEPSPVYTAYSNLHLYLERKRIQARQALRADAKLLTTLASSVLDPSYIAPRTTDPNTETESDPSGTAAATVYRPEGQGPRVMILGPESAGKTSLVKLLANYALRSPAVASLGKGEAAKVAESLRTGGDGIIYPNMEDNLSEEAKKQKREEEKRSDITGWWPVVVNLDPSDGAPPLPCCLSALPLSPLPLASLPSASPAYAFGTNTSTTGAIPPGTSTAHGVAPLSLWLGKENLRENERHFRRVVDWLAEGVERRFARDFRSRMSGLIIDTPGVITADARNKYAFIQHCVKAFKVDTIVVLGHEKLNLEMTKLFASPAVTTVETAEIPGSAGQRLPRVNVIKLPKSGGVVELDETYRSRLKALQVKTYFYGGSTSGSANTDGGVPKPVLPGHSDPLGGVPSLSPYSTTIPFDLLEIYKVGQESLAPSSALPIGASRTVTETQLVKLDPTNSAADQTSLLHSVLALIQPPRGGGGAGQPDSSTNPTDDEIIGAPILGFVHVADIDTVRKKITVLSPSAGRLPSKTAIIGSLDWQDV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein localizes to the mRNA cleavage factor complex. The designed protein sequence is ",
    "response#1": "TTRRLHLLTQHELNGTITGLAATRTLESTIDGLDRLIVSFKDAKMALLEWSRGDIATVSLHTYERCSQMNTGDLQSYVPLLRTDPLSRLAVLTLPEDSLAVLPLIQEQSELDPLSEGFSRDAPYSPSFVLSLSDMSITIKNIQDLLFLPGFHSPTIALLFSPMHTWSGRLQTVKDTFCLEIRTFDLSSGTSYPLLTSVSGLPSDSLYLVACPSELGGIVLVTSTGIVHVDQGGRVTAACVNAWWSRITSLKCSMASVSQKLTLEGSRCVFVTPHDMLLVLQNGAVHQVRFSMEGRAVGVIEVLDKGCVVPPPSDLTVAGDGAVFVGSAEGDSWLAKVNVVQ",
    "response#2": "TTRRLHLLTQHELNGTITGLAATRTLESTIDGLDRLIVSFKDAKMALLEWSRGDIATVSLHTYERCSQMNTGDLQSYVPLLRTDPLSRLAVLTLPEDSLAVLPLIQEQSELDPLSEGFSRDAPYSPSFVLSLSDMSITIKNIQDLLFLPGFHSPTIALLFSPMHTWSGRLQTVKDTFCLEIRTFDLSSGTSYPLLTSVSGLPSDSLYLVACPSELGGIVLVTSTGIVHVDQGGRVTAACVNAWWSRITSLKCSMASVSQKLTLEGSRCVFVTPHDMLLVLQNGAVHQVRFSMEGRAVGVIEVLDKGCVVPPPSDLTVAGDGAVFVGSAEGDSWLAKVNVVV",
    "response#3": "TTRRLHLLTQHELNGTITGLAATRTLESTIDGLDRLIVSFKDAKMALLEWSRGDIATVSLHTYERCSQMNTGDLQSYVPLLRTDPLSRLAVLTLPEDSLAVLPLIQEQSELDPLSEGFSRDAPYSPSFVLSLSDMSITIKNIQDLLFLPGFHSPTIALLFSPMHTWSGRLQTVKDTFCLEIRTFDLSSGTSYPLLTSVSGLPSDSLYLVACPSELGGIVLVTSTGIVHVDQGGRVTAACVNAWWSRITSLKCSMASVSQKLTLEGSRCVFVTPHDMLLVLQNGAVHQVRFSMEGRAVGVIEVLDKGCVVPPPSDLTVAGDGAVFVGSAEGDSWLAKVNVVP"
  },
  {
    "reference": "MSEFVTVARPYAKAAFDFAVEHQAVERWQNMLAFTAQVTRNEQIAELLSGAVAPETMSTTFIAVCGDQLDEPAQNFIRVMAENGRLLVLPEVLQQFIQLRASLESTVDVEVSSARALNDEQLAKIAAAMEKRLSRKVKLNCKIDKSVMAGVVIRAGDMVIDGSVRGRLERLADVLQS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be involved in proton motive force-driven ATP synthesis by localizing to proton-transporting ATP synthase complex, catalytic core F(1), plasma membrane and exhibiting proton-transporting ATP synthase activity, rotational mechanism.2. For general function, the protein need meet that This protein is part of the stalk that links CF(0) to CF(1) The designed protein sequence is ",
    "response#1": "SCHFG",
    "response#2": "GNITC",
    "response#3": "MR"
  },
  {
    "reference": "MSQKDIYNKYSNQSADDQQEEDDDENQESSCQLSPEKDLGESLQGEDLDEDQGVIGEDDEEQEESYDEEDEEEDDEENNQDQQNNSESNLQYDKTNQKNQQTEMTDNQNEFLAEDYLSQIKTNEKSELKYQLDIQSNLSGENVQDMDEKLKQYIELNGKNENQQAAESKKIENDEIMKKSEENVHEKVVDNNQKLNIQQNNINDYQEDIKKDQSNNLVDIQQNNEIIKEVNSEDQQQKNEMDNKQSDIVLAQEESKQSEQVEAPIVKQKKIELRPKVIYDPKNDVDQYTGDYSDSGESDEEANNEDDDEDEDDESENESRSRKNKAQLLKKKNNKKEQAKKQQVKKVYKKQTLVLNVADTKYPVVKFVGKKIFKWKLAYDMESMDFDIFWTDNAVQPEQLGRMQPYQKINHFPGMFSLARKNHLARNLMKMRKQFPDQYKFFPQTWLLPAEYNDFKNQFEKSRSQQKIFIVKPEASCQGRGIFLTRSLDDLNPSDHYVVQRYLNKPYLIDGLKFDFRLYVLLAGCDPLRIYLYYEGLTRFATEKYQEVNRDNIEDMCMHLTNYAINKDNPNFKFNKDKEKMDVGHKRSLTSVLQLLEDQGHDVNKLWKDIKRVLIKTIISAQPTLAHHYKSCQPDNFMNNMCFEILGFDIILDSHLKPWVLEVNHTPSFSTDTPLDSYIKKNTIRDSLKLMNCTCKAKNEIINQRKEIMQKRVLTGKKVKYTPEEKLEEIKKAQQKRDEYEDKHLGGFERIFPMEDDEDDNFTEYMQY",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The presence of TTL domains is critical for the interaction of the designed protein with its intended targets.2. The protein should be optimized for cytoplasm, cilium-specific folding and stability, while maintaining tubulin binding, ATP binding.3. Prioritize Basic and acidic residues, Basic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "CLGIRSFGDAQGCTELLNVAHKYTMEHFIEVIKNQEFLLLPANEISKLLCSDDINVPDEETIFHALMQWVGHDVQNRQGELGMLLSYIRLPLLPPQLLAC",
    "response#2": "LYAVGGMDAMKGTTTIEKYDLRTNSWLHIGTMNGRRLQFGVAVIDNKLYVVGGRDGLKTLNTVECFNPVGKIWTVMPPMSTHRHGLGVATLEGPMYAVGGHDGWSYLNTVERWDPEGRQWNYVASMSTPRSTVGVVALNNKLYAIGGRDGSSCLKSMEYFDPHTNKWSLCAPMSKRRGGVGVATYNGFLYVVGGHDAPASNHCSRLSDCVERYDPKGDSWSTVAPLSVPRDAVAVCPLGDKLYVVGGYDGHR",
    "response#3": "CLGIRSFGDAQGCTELLNVAHKYTMEHFIEVIKNQEFLLLPANEISKLLCSDDINVPDEETIFHALMQWVGHDVQNRQGELGMLLSYIRLPLLPPQLLADLC"
  },
  {
    "reference": "MKPRFDIIRASRWFFLLSGAITVAGVIVFALFGFNLSTDFKSGSEVQFELNQRVPEARVRQMFASIGLPLGDTSLTVGGIQQNVVMVTLPEQLTAKQISEIQAAEHRFFPDAKQDIQVNSVDPFVAEQTARKAVYAVLAAAACIVVYIAIRFEFRFAISGIIALLHDAFIVLAAFALLRREVDLTFVAALLTIVGYSINDTIVIFDRIRENLKIDKPETVDELRAVVNKSLWQVMNRSIRTVLTVLIAAVILYFFGGISIRNFTFALIIGLVSGAYSSIFIASPIWVAWRSRSMKKATRGDKAAPIPE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The designed protein should have protein-transporting ATPase activity within plasma membrane to regulate protein targeting, protein transport by the Sec complex.3. For general function, the protein need meet that Interacts with the SecYEG preprotein conducting channel; SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.; Part of the Sec protein translocase complex The designed protein sequence is ",
    "response#1": "LKGEAAEKFGKFTEQNIGKRLAIVLDRKVVSAPVIRSKISDRGQITGNFTAQEARDLALILRTGSLPSPLKFLQEKIVGPSLGKDAIEQGIKAGILAIILLAVVLIARYKTAGITANISIFLNVLFLLASMAFLGATLTLPGIAGIILNMGIAVDSNVLIFERVKEELRLGNTVSKAIELGFKRTLSAVWDTHVTLLVASVILFQFGSGPVKGFATTLALGTIASFISNL",
    "response#2": "LKPEAAKKFADFTTQNVGKYLAIALDKKIISSPQIRDPITNGRGVITGVTREEARTLAIQIRSGSLSVPLDIISSNTVGATLGNDSVQRSIRAGLIGIGAVALFMILYYRLPGFVSVVALAIYAAVVFALFKLIPVTLTLAGIAGFILSVGMAVDANVLIFARLKDELRRGRGLIQAIDVGFKNAWPSIRDSNISTLITCAILIWFGSRFGASVIKGFAITLAIGVIVSMFTALFSNYFFN",
    "response#3": "FNSLGSKLFGEVTKNNVGKHLAIVLDNKLLSAPTINQPIMGGSGIISGDFTVESANELALLLRAGSLPAPLKIIEERSIGPNLGADSIESGKKAGIIGFAAVCIFMVWSYGLLGLFANIALSLAMLYVLALLSLFQATLTLPGIAGIILTMGMAVDANVLIYERIKEELNKGTSNLYAIKTGFESAFATILDSNLTTLIVAFLLYIFGVGAIKGFAVALTIGIISSMFSALFSNYFFN"
  },
  {
    "reference": "MSSLFDAVLQSELGSTAGSRDHRLHSDQIPSSRPQPPSESNGPMSDMHGFADDQIADSSASTVTRLRNPYLPGPPPVSDVAGEKVQQAFAELLETYQEETPSSAQAAPSSQVLSEKYYIAQIMGMAKWELSTLYVDFTHLTSMSNPILADAIANQYYRFQPFLTKALHDLIAKYEPDYFASHRQATAAGSVSSQAGTSMIAGNSSVSDNPELERNIREKTRHQQTDKLFSLAFYNLPLVSRLRQLRTSQIGKLVSVSGTVTRTSEIRPELSLGTFICEECKAVVTNVEQTFRYTEPSQCPNNTCGNRSGWRLDIGKSTFVDWQKVKLQESSHEIPTGSMPRTMDVILRGEMVDRAKAGERCIFTGTLIVVPDVSQLGLPGVRPEAVRDDGAFRGSDIGGGGVTGLKALGIKDLTYRLAFLSCMVTPDTTTPGQQSNQQLNGQSQNILASLNQNRDPESNEDQAQEALLQSLTPYEVQDLKNLVHSEYIYSRLIDSIAPMIYGHRQIKKGLLLQLIGGVGKSTEQENLQLRGDINICIVGDPSTSKSQFLKYICSLHPRAVYTSGKASSAAGLTASVVKDAETGEFTIEAGALMLAVGGICCIDEFDKMDISDQVAIHEAMEQQTISIAKAGIHTTLNARASILAAANPIGGRYNPKTTLRGNLNFSAPIMSRFDLFFVIRDDPNETVDRNLADHIVNVHMNRDEAVNPELSTEQLLRYIRFARTFKPVFTEEAKAYLVEKYKELRAGDAQGGMGRSSYRITVRQLESL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity..2. The protein should be optimized for stability and expression in replication fork protection complex where it will participate in pre-replicative complex assembly involved in nuclear cell cycle DNA replication.3. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "GIPTGFVQLDNYTSGFNKGSLVIIGARPSMGKTSLMMNMVLSALNDDRGVAVFSLEMSAEQLALRALSDLTSINMHDLESGRLDDDQWENLAKCFDHLSQKKLFFYDKSYVRIEQIRLQLRKLKSQHKELGIAFIDYLQLMSGSKATKERHEQIAEISRELKTLARELEIPIIALVQLNRSLENRDDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAEDNKIDKLKKEGKIEEAQELYLKVNEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPFTRYET",
    "response#2": "GSLEVTGIPTGFVQLDNYTSGFNKGSLVIIGARPSMGKTSLMMNMVLSALNDDRGVAVFSLEMSAEQLALRALSDLTSINMHDLESGRLDDDQWENLAKCFDHLSQKKLFFYDKSYVRIEQIRLQLRKLKSQHKELGIAFIDYLQLMSGSKATKERHEQIAEISRELKTLARELEIPIIALVQLNRSLENRDDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAEDNKIDKLKKEGKIEEAQELYLKVNEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPFTRYEDMPIDR",
    "response#3": "GIPTGFVQLDNYTSGFNKGSLVIIGARPSMGKTSLMMNMVLSALNDDRGVAVFSLEMSAEQLALRALSDLTSINMHDLESGRLDDDQWENLAKCFDHLSQKKLFFYDKSYVRIEQIRLQLRKLKSQHKELGIAFIDYLQLMSGSKATKERHEQIAEISRELKTLARELEIPIIALVQLNRSLENRDDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAEDNKIDKLKKEGKIEEAQELYLKVNEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPFTRYES"
  },
  {
    "reference": "MKSIFDFKLEDLTSYLEDNGYSKFVSKQIYDWIYKKRVFDFDKMSNIKLELREQLKKDFDISMLKLVRRQTSKETNKYLFELDDGNLIESVLMRHDYGLSVCVSSQIGCNMGCSFCESGRLKKVRDLLPGEIVRQILMIEEDVGMRISSVVVMGIGEPFDNYDNIMDFIRIINSPFGIAIGARHITVSTSGLVPMIKRYAEEDLQTNLAISLHAPNDELRNKIMKVNKAYNISELIAAINEYIEKTNRRVTIEYVMLSMVNDSEENANELADLLRGMNVYVNLIPYNETSHIEYKKSPNDRVMKFYDTLKRRGINVTVRREFGGNIDAACGQLRANEVER",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein is the optimization of the Proton acceptor, S-methylcysteine intermediate to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.2. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.3. The ideal protein would be one that plays a critical role in rRNA base methylation through tRNA (adenine-C2-)-methyltransferase activity, rRNA binding, 4 iron, 4 sulfur cluster binding, tRNA binding within cytoplasm.4. The protein contains novel Radical SAM core domains that confer a unique function or activity.5. The protein should have a high affinity for [4Fe-4S] cluster.6. The protein must bind to [4Fe-4S] cluster in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "QSVCVTTQVGCNIGCSFCASGLLRKSRDLQAGEIVEQIMNVQEHLDALGGGERVSNIVVMGIGEPFDNYDNLMNFLRVVNDGNGLGIGARHITVSTSGLVKGIRKFADEDLQVNLALSIHAPNNELRTKIMKINKAFPLEQVMDAIDYFLSKKNRRITFEYILLSDVNDHVEEAQQLAKLLQNKKHLSYVNLIPYNPVEEHDRYSRSTPEAIAAFEKEFFQNCDYPHPAISDVSDAALLAWLRKRGEPAYRAKQIRRHATHGTAAEFAELTDLPKQLREALAASFSWTSVDPVREDASADGETRKALLRLADGHHIETVLMPHHGARNAVCVSTQAGCPMACAFCATGEMGLVRNLTAGEIVDQVRHWQRELAQRGERVSHVVFMGMGEPFNNYDATLASTRALIDPEAFGISPRRLTISTCGVVTGIDQLAGEGLPINLAVSLHAPDDPTRARIMPINRKWGVEEVLAAAARYVKRTKRRVTFEYVLLAGVNDSEETARELAALITRHGPTKDAHVNLIPYNPGAGGFRRPSVERMERFAALLQEHGIAATLRISKGQDIAAGCGQLKVAEA",
    "response#2": "TTQVGCNIGCSFCASGLLRKSRDLQAGEIVEQIMNVQEHLDALGGGERVSNIVVMGIGEPFDNYDNLMNFLRVVNDGNGLGIGARHITVSTSGLVKGIRKFADEDLQVNLALSIHAPNNELRTKIMKINKAFPLEQVMDAIDYFLSKKNRRITFEYILLSDVNDHVEEAQHPAISDVSDAALLAWLRKRGEPAYRAKQIRRHATHGTAAEFAELTDLPKQLREALAASFSWTSVDPVREDASADGETRKALLRLADGHHIETVLMPHHGARNAVCVSTQAGCPMACAFCATGEMGLVRNLTAGEIVDQVRHWQRELAQRGERVSHVVFMGMGEPFNNYDATLASTRALIDPEAFGISPRRLTISTCGVVTGIDQLAGEGLPINLAVSLHAPDDPTRARIMPINRKWGVEEVLAAAARYVKRTKRRVTFEYVLLAGVNDSEETARELAALITRHGPTKDAHVNLIPYNPGAGGFRRPSVERMERFAALLQEHGIAATLRISKGQDIAAGCGQLKVAAQ",
    "response#3": "ISTQAGCPLKCAFCLTGIAGYKRNLKTWEILGQVAVVMEEGPREHLPWNVVVMGMGEPLLNYEATVSALRVLMDPEGFAVAPRKLTLSTVGILPALEKLMQEPVRPNLAISLHAPNQALRRVLMPIEEKYALADVMTAALRYPIPRGGLVTFEYVLLRGVNDRSHVERVLEDDRATICLSCQAGCKMGCIFCLTGKQGFQGNLSSNEILNQFRSIPEFRKLTNIVYMGMGEPLDNLDEVIKSLEILTGLWGYGWSPTRITVSTIGLKNQVREFLERSRCHLAVSLHSPFDEERSRLMPIQKTNNIREILEIIRTADLNSQRRISFEYILFKGINDTPQHVQELARLLNGIKCRINIIRFHTIPGSEFTSPSLQEAEGFRDILNKKGILATVRRSRGEDVSAACGQLKR"
  },
  {
    "reference": "MNLKQHTIQKPVTVSGVGLHTGVIASMTFQPAPVNHGYKFQRIDLPGQPIVDADVDFVVDVSRGTTLEHNGARVHTVEHTLAALVGLQIDNCLIQLDGPEPPIMDGSSIKFIEALDDAGLVEQNALRNYFEVPSEVRYRDRDRDVEIAALPLDDYRVTVMVDYNSKVLSSQHASLNDITLFKQEIARSRTFCFLHELEALFKANLIKGGDLSNAIVIVDREFEDGELDHLSSLLGKPRVSVVPGKGILNNIDLHYANEPARHKLLDVVGDLALVGRPLKAQILAARPGHAANVEMAKKIKKLMRETQKPSIPVYDPKVPPVMDIKQISNLLAHRYPFQLIDKIIHMDESSVVGVKNVTINEPFFTGHFPENPVMPGVLQIEAMAQTGGILVMSTVPDPENYWAYLAAIENCRFRRNVVPGDTVIFKCEFMAPMKRGIAKMHGQAWVAGQLVMESDMTASLVRKK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity functions is required to enable lipid A biosynthetic process.2. The protein should be designed to have high stability and activity towards a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine +   H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate. The designed protein sequence is ",
    "response#1": "MLIGLILLAIGAGFGLYTVLK",
    "response#2": "MGKVIGVGTKFDLTDEQRAWLAPVLPALPRKRGGRPPKHPRRILLDAVMYVLRTGCAWRLLPADFPKWQTVYWYFRLWQADGTWDRIHAALVARVRKAAGRDAAPSAGVIDSQSVKTTEKGGPRGYDAGKKINGRKRHIVVDTLGLVLVVHVTPADVQDRDGAKVVLDAARDKFPRVRWVWADAGYAGGKLVDWARKTLGLEVAIVSRSDRAKGFVVLPRRWVVERTFAWLGRCRRLARDFETTLASSQAMVYIAMIRLMLRRLA",
    "response#3": "LFTK"
  },
  {
    "reference": "MPRTVSNVFIETLITAGVKRVYGVVGDSLNGLTDVIRKSKKIDWVHVRHEEVAAFAAGAEAHLTGEIAVCAGSCGPGNLHLINGLYDCHRNRVPVLAIAAQIPSSELGSGYFQETSPEHLFKDCSHYCELVSQPEQIPRVLGIAMRAAIAKRGVAVVIIPGDIALRDCPSPPLSLGLGDSASTTLPPVDALKKAAALLNSAKKVAILGGAGCAGAHSELIAIAGRLKAPIVHAMRGKEFIEYDNPYDVGMTGLLGFSSGYHTMMNCDALLMLGTDFPYQQFYPKDATIIQVDLRGEQIGRRTPVDVGLVGSVKETLQALTPLVNDKADRSYLDVCLKHYGQARKGLDELATGEPGRTPIHPQYVAKVLNELASDSAIFTCDVGTPTIWAARYLRMNGKRRLLGSFNHGSMANALPQAIGAQAAFPDRQVVSLSGDGGLAMLLGDLLTLRQLKLPVKVIVFSNSSLGFVELEMKAAGLLDYGTELVNPNFAKLAESAEILGFRVEAPEALQPALEAALAHEGPCLVEVIVNRQELAMPPTITATQAIGFSLYMIRAVLSGRGDEVIDLAKTNLFSR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein have Thiamine pyrophosphate enzyme TPP-binding, Thiamine pyrophosphate enzyme N-terminal TPP-binding domains with specific sequence motifs that are crucial for its activity.2. The protein can be expressed in plasma membrane and perform flavin adenine dinucleotide binding, ligase activity without triggering undesirable cellular responses.3. The protein should be able to bind FAD, Mg(2+) ligand in a variety of conditions4. The protein should be designed to have high stability and activity towards a ubiquinone + H2O + pyruvate = a ubiquinol + acetate + CO2.5. The protein must exhibit the following characteristics: A peripheral cell membrane enzyme that catalyzes the oxidative decarboxylation of pyruvate to form acetate and CO(2); It channels electrons from the cytoplasm to the respiratory chain at the cell membrane via ubiquinone..6. The stability of the protein-thiamine diphosphate complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "MTVADLLAKTLASAGVSRIWGVTGDSLNGLNDSLRRFDQIEWMHVRHEETAAFAAGAEAAITGKLAVCAGSCGPGNLHLINGLFDCHRNRTPVLAIAAHIPSSEIGLGYFQETHPEELYKVCRDFCERPLHPQFVAATLDRLAADDAVFTFDVGTPAIWAARYLRMNGQRRLIGSFNHGSMANAMPQALGAQAAQPGRQVVSLSGDGGLAMLMGDMLTAVQMKLPIKIVLINNGVLGFVQMEQKASGFLDTNVSLQNPDFSAIAGGMGFLGLRVTRSDALEAALAQALAHDGPALVDVVVDSMELPVYPG",
    "response#2": "MTVADLLAKTLASAGVSRIWGVTGDSLNGLNDSLRRFDQIEWMHVRHEETAAFAAGAEAAITGKLAVCAGSCGPGNLHLINGLFDCHRNRTPVLAIAAHIPSSEIGLGYFQETHPQELFRECSDFCEMVPLHPQFVAATLDRLAADDAVFTFDVGTPAIWAARYLRMNGQRRLIGSFNHGSMANAMPQALGAQAAQPGRQVVSLSGDGGLAMLMGDMLTAVQMKLPIKIVLINNGVLGFVQMEQKASGFLDTNVSLQNPDFSAIAGGMGFLGLRVTRSDALEAALAQALAHDGPALVDVVVDSMELPPVPSEEQLARAARLLNEADRVTILAGAGTAGAHEEVIRAAGTLAAPIVHALRGKEYIEYDNPYDVGMTGLLGFASGYKAIEEAGVLLMLGTDFPYQQFYPENATIIQVDIRGRHLGRRTPIDLGLVGTVADTLTARLMQERGDVAAAAAVARAARLLNEADRVTILAGAGTAGAHEEVIRAAGTLAAPIVHALRGKEYIEYDNPYDVGMTGLLGFASGYKAIEEAGVLLMLGTDFPYQQFYPENATIIQVDIRGRHLGRRTPIDLGLVGTVADTLTARLMQERGDVAAAAAVDELAQFLNAGKRVTLLCGAGCAGAHSEVVALAKQLKAPIVHALRGKEHLEYDNPYDVGMTGLIGFASGFNAMKECDTLLMLGTNFPYRQFFPEHSRIAQIDLQPEALGNRCPLAIGLIGDIKHTVQQLVEEHGDLPRAAE",
    "response#3": "MTVADLLAKTLASAGVSRIWGVTGDSLNGLNDSLRRFDQIEWMHVRHEETAAFAAGAEAAITGKLAVCAGSCGPGNLHLINGLFDCHRNRTPVLAIAAHIPSSEIGLGYFQETHPEELYKVCKDFIDITVAEQIVATLAASGVRRVYGVPGDSLNGITDALRKDGRIAWVHVRHEEAGAFAAAADAATTGELAVVVGSAGPGNLHLINGLYDANRSRVPVLAIAAHIPTAEIGTGYFQETHPQELYKICKDFIDIISMYFAKLLEKVGIKRIWGITGDSLNGLSDSLEKLNKIKWIGTRHEETAAFAAGADAKVSGEIAVCAGSCGPGNLHLINGLYDCQRKGVPILAIASHIPSSEIGSGYFQEHPKEFYKICKDFVDLIAVQQAAAILNDAGAVTILAGAGVEGSHDAVITAATTLNAPIVHALRGKEFIEHDNPHDVGMTGLLGFPSGYKAIKEADALLMLGTDFPYQQFYPDNAKIIQVDIRGRNLGRRTPIELGLVGSVRDTLAALAEEMGLDAKDIKEGLERLAALLNGGGRVTILCGSGCQGAHEELLALGERLKAPMVHALRGKEHVAWENPYDVGMTGLIGFSSGYYAMRDCDVLLMLGTDFPYRQFYPEGAGVRIAQVDLRPEQIGRRTPVDLGVVGDVRATLKALYGVGDIDKCMEVARAAELLNGAEKVAILAGSGCAGAHDQVLALAEALQAPVVHALRGKEYVEWDNPFDVGMTGLIGFSSGYHAMMNCDALVMLGTDFPYRNFYPSDARIIQIDHDPSSLGKRAHLAQGVVGDVAETVAA"
  },
  {
    "reference": "MKLVFATNNPNKLKEIQHKLGDEIELLGLKDIGCTEDIPETAPTLEGNALQKAMYVYQNYGYDCFADDTGLEIDALNGEPGVYSARYAGQAKDAQANMAKVLEKMNGEHNRIARFRTVIALVIGGKQTWMDGEVKGTILTEKRGAEGFGYDPIFQPEGYELSFAEMDLNEKNKISHRARAVEKLVTFLNRS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The Mg(2+), substrate binding site should be stable and able to withstand changes in environmental conditions.2. The presence of Mg(2+) is necessary for the protein to function in the desired environment.3. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.4. The designed protein must possess dITP diphosphatase activity, ITP diphosphatase activity, nucleotide binding, ribonucleoside triphosphate phosphatase activity to support nucleotide metabolic process, purine nucleoside triphosphate catabolic process under certain conditions. The designed protein sequence is ",
    "response#1": "MEIILATRNAGKLREFAAAFRPLGLSLRAASEFPDLPEVVEDGTTFAENALKKARAAAAATGLPALADDSGLCVDALGGAPGVWSARWAGRHGRDRVNNRLLLAQLADVPDAQRTARFHCVLVVVRPDGSEQVAEGRWPGEILRAPRGDGGFGYDPVFVPLGDTRTCAELAPAEKNAISHRGQALRQLLADLRS",
    "response#2": "MEQRKVRI",
    "response#3": "MKILVLNGPNLNLLGTREPETF"
  },
  {
    "reference": "MNYQFTSNLQQAQQLDQEDPLAKIRDHFYIQPGQIYMDGNSLGLASKEAETALLKMMNIWKTQGINMWDEYFHYGLELGAQCATLINARANEVLITGSTTSNVHQCISTLYQPTAERYKILVDDLNFPTDRYAIDSEIRLRGYNPEEAVKVIPSPDGKYIDEDAVIAGMSDDVALILLPTVLYRSAQVLDMERLTKAAHERGIIIGWDLCHAIGAITMDFQKIQPDFAIWCTYKYLAAGPGSIAGMYLNQKHFDKQPGLAGWWGNRDDTQFQLKQNFEHQKDVSGWQIGTPSMLSMAPLEGTLKLINSIGMEAIRTKSLKITAYLMYLIDEKLTQYGCKVGNPREDSKRGGHVCLEHPQGYQISLALKDRNVIPDFREPDVIRLAPIALYTSYEEVYQMVDILEDILKTKSYEKYSAKRGLVV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a binding site that is specific to the target pyridoxal 5'-phosphate ligand.2. The protein design should consider the endogenous proteins and enzymes present in the quinolinate from L-kynurenine: step 2/3, NAD(+) biosynthesis pathway to avoid interference with native metabolic processes.3. The protein should have a fast turnover rate for H2O + L-kynurenine = anthranilate + H(+) + L-alanine.4. A protein with pyridoxal phosphate binding, kynureninase activity, 3-hydroxykynureninase activity is needed to enhance anthranilate metabolic process, quinolinate biosynthetic process, tryptophan catabolic process, L-kynurenine catabolic process, 'de novo' NAD biosynthetic process from tryptophan in a specific context.5. For general function, the protein need meet that Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively.6. The designed protein have Aminotransferase class V domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MNSTITREGPSIALILFSGVQFYTGQAFPIKQITAAGHQQGCIVGWDLAHAIGNVLLELHDWQVDFAAWCSYKYLNGGPGAIAGLFVHEKWTSDPTKLGLTILNVCGDPAAEYGKVVRDLDGNIVRIVEFKDATDEEKSATLCNSGVMAIDAKIMFDLIDKIDNDNAAGEYYLTDIVEIARSKGLKAAVTVCDEAETLGINTRAQLAAAEARFQERARKEAMLDAXVTFVDRFGVSIEDIKRKYADMKAKK",
    "response#2": "MNRHDLNQSNHTTHPAQVASLPGLTAQPAAPASANNRANGGQTPPAAMDFEHIAGLIEQALRANKGTLLIGDDDLHAIGLPVARIAQPQSLKGITAVARQLMGSTERIIGIKGPRASGKTTLARALVAHLLAHDLKPGSIALLDGKNVANGALPAGGFAANRFNITRENMALTTLCLAQAGQTAISVAETSGHAVGQQIAELCKTHNVRPIYLVVDLDKPTVHEDRTRDQQMIAESCGQGNSAGLADRIDASYAAATALDLSHADERRTREQLVARIAALLAEAGILQPFETAAGSAASALRAAAANAPAAAAAAGPAAAPAAAAVAPAAAPAAPAASAAKPVKPAAKPAAAPAPAAAAAPAAAPAAAVAAAAASAANGAAPAASAAPAAPAAPAAAQAAATPAAATPAAPAAPAAAGPAATTAAKAAAKPADKPAAKPGKPAAKPAAAKPAAKPAAAKPAAKPAAAPAAKPAAKSAAKPVAKPAAKPKAAAPAAAPAAKPAAKPAAKAAAKPAAKPAAAKPGAKK",
    "response#3": "MTSTITREGPSIALILFSGVQFYTGQAFPIKQITAAGHQQGCIVGWDLAHAIGNVLLELHDWQVDFAAWCSYKYLNGGPGAIAGLFVHEKWTSDPTKLGIVTNADDSVSLPGRTFFARDGQVVTEQVEKKDFPPFPLPPVAIYDPIHEPPADLAGRAASPDEVAYVIYTSGSTGKPKGVVVSHRALANYLAWARRHYGIGPEDRVYQGFSTSFDISFWEMFGPLASGARIVLAPEEARKSPQALVELIVQERVTVLHFVPVLLRAFVDGAALDGCASLRRVFCGGEALPPDVSRAFAARLPHVELHNLYGPTEAAFDASFHACRAADDATPIGVPIGRPAPNVRIQILDAALQPVPVGVPGELCIGGAQVAQGYLNRPELTAERFIADPFNPVAAPGARLYRTGDLARWLPDGSVEYAGRIDHQVKIRGYRIELDAIEAALLRHPGVREAVVLAREDAPGDKRLVAYVVGREAADAPTALRDELRSHLRETLPPYMVPSAFVTLDALPLTANRKVDRKALPALPRETAAPADTGFVAPRNELEAKLAEVWEEVLGVPRVGIRDNFFELGGHSLLAIRVMARIRQALGVELPLATLFEAPTIAELAARIEAARAERADALPALAPVRRGDELPLSFAQQRLWFLAQLEPDSAAYNIPLAMRLKGPLDREALRRSLEQIVRRHEALRTT"
  },
  {
    "reference": "MKRSLLEIERIAYKPKLPALFQQADSFNAKLGAAMESAGDHEQVKKHFPNTYGMPIVSFEAGQSKESKPLNLAVILSGGQAPGGHNVISGLFDALKKHHHKSKLYGFLGGPSGLIENKYREISKELLANFRNTGGFDIIGSGRTKLESPEQFDKTRQHLESLHINALVIIGGDDSNTNAGILAEYFLANKVNINVIGVPKTIDGDLKNEYIETSFGFDTATKVYSELVGNIARDAKSAKKYWHFIKLMGRSASHITLECALQTQPNIALIGEEAANFNHSLKAIVNDMADIIAKRADNGENFGVALIPEGLIEFIPEMRKLIDELNELMVIHQIELEKLESLDEKISFIREHITAESKELLDILPVEIARQLALDRDPHGNVQVSRIDTEKLLLEMVKIELKRRKKEGSYAGKFSAQNHFFGYEGRCSMPSNFDADYGYSLGYTAAKLAICGLTGYMSCIRNLSRPAIEWQPGGVPITSMLNMERRHGEDRPVIQKALTKLDGEPFLKFKANREKWSLETSYVYPGPIQYYGPDAVCNRTTISLELENQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein with the reaction beta-D-fructose 6-phosphate + diphosphate = beta-D-fructose   1,6-bisphosphate + H(+) + phosphate, the Proton acceptor must be optimized to ensure efficient catalysis.2. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.3. The protein must have high specificity towards diphosphate-fructose-6-phosphate 1-phosphotransferase activity, 6-phosphofructokinase activity.4. We require a protein that can specifically bind to the metabolites produced in the glycolysis pathway.5. The designed protein must have at least one Phosphofructokinase domain.6. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+) binding. The designed protein sequence is ",
    "response#1": "RVGVLTGGGDCPGLNAVIRAVVQRAIKSYGWSVAGIRHGWRGLLLEEIEPLDLSSVSGILPRGGTILGTSRTNPYKEQDGESRIRATLARREIDAVVVIGGEDTLGTAVKLHGAGVPVVGVPKTIDNDVNGTDYTFGFDTALTIATEAIDRLHTTAESHDRVMVAEVMGRHAGWIALCSGIAGGADMILIPEFPIPFHEICEAIWRRHERGKNFSIVVVAEGALLADEAGNPLHVQTEKRDAFGHVRLGGIGASLAEKIEETTGYETRYSVLGHIQRGGSPTAFDRVLGSRFGVAAVDLIDAGVVPQLVALRRVGVLTGGGDAPGLNAAIRGVARAAWREGYEVVGIRNGWQGLLRNDVFPLTPDAISGILPRGGTILGTSRTNPFKTSEGPRQVLDTVRTRGIDVVVAIGGDDTLGVAARLAKMGMRVVGIPKTMDNDIPGSDYTIGFDTAVTAVMEACDKLHPTAEAHQRVMVVEVMGRDAGWVAAVGGLAGGADVVLVPEVPFSVDEVCQRIKDRHERGKLFSVLVVAEGCKPKDLGTQVAQDAAVDAFGHVRLGGIGSVLAGEIEARTGYETRVTVLGHLQRGGSPTWFDRVLATRFGVAAVELLATGAVGQMVSLRGRVGVLTGGGDAPGLNAAIRGVARAAWREGYEVVGIRNGWQGLLRNDVFPLTPDAISGILPRGGTILGTSRTNPFKTSEGPRQVLDTVRTRGIDVVVAIGGDDTLGVAARLAKMGMRVVGIPKTMDNDIPGSDYTIGFDTAVTAVMEACDKLHPTAEAHQRVMVVEVMGRDAGWVAAVGGLAGGADVVLVPEVPFSVDEVCQRIKDRHERGKLFSVLVVAEGCKPKDLGTQVAQDAAVDAFGHVRLGGIGSVLAGEIEARTGYETRVTVLGHLQRGGSPTWFDRVLATRFGVAAVEL",
    "response#2": "NVGVILSGGQAPGGHNVIAGIFDGIKRLHKESKLYGFILGPAGLIDHQYKELTSEIIDEYRNTGGFDIIGSGRTKLETKEQFDKGLIILKKLDIKALVIIGGDDSNTNACVLAEYYKSINAGVQVLGCPKTIDGDLKNEQIETSFGFDTACKVYSELIGNIQRDCNSSRKYWHFIKVMGRSASHIALECALQTHPNVCIISEEVEEKDLSLDDIIEYIAGVIAKRAENGLNFGTVIIPEGLIEFILSSGAKMGIIAGRIGILTGGGDCPGLNPVIRAVVRKSLIEGYEIIGIKNGWKGLVENDTMKLDLTAVSGILPKGGTILGTSRTNPFKKPEDLKKVQDNFKKLGLYALVAVGGEDTLGAASKLIKNGIKNIVGVPKTIDNDLSCTDYTFGFDTALNIATECIDRLHTTAESHHRIMVVEVMGRHAGWIAIESGMAGGADVILIPEKPIDMDEVCDIIKRRHGRGKTFSIVVVAEGAQFKEKDMVLKEEKLDAFGHVRLGGIGEILAKEIESRTGYETRVSVLGHIQRGGTPTAFDRVLGTRMGVKAVELVKNKEFGSMISLRGNEMVSVPLSDAVTDLKRVDGDWYEFGRFL",
    "response#3": "RVGVLTGGGDAPGLNAAIRGVARAAWREGYEVVGIRNGWQGLLRNDVFPLTPDAISGILPRGGTILGTSRTNPFKTSEGPRQVLDTVRTRGIDVVVAIGGDDTLGVAARLAKMGMRVVGIPKTMDNDIPGSDYTIGFDTAVTAVMEACDKLHPTAEAHQRVMVVEVMGRDAGWVAAVGGLAGGADVVLVPEVPFSVDEVCQRIKDRHERGKLFSVLVVAEGCKPKDLGTQVAQDAAVDAFGHVRLGGIGSVLAGEIEARTGYETRVTVLGHLQRGGSPTWFDRVLATRFGVAAVELVCNDHFGVMVSLRGTEIVRVPLADGTSALKTVPAERYEEAEVLFG"
  },
  {
    "reference": "MSSQAPLVFIMAAGLGTRMKSDTPKVLHKVAGRPLLHWVVTAARAAGAGKIVAILGHKSELVKVALDASFGVDAIGIAIQAEQRGTGHAVQCALPAVANEPDDRIVVILTGDAPLLRSERIAELARACQESPAGMALLSTVPPRPMPYGRLVREDGKLTRIVEHSDATPEQKLIADTNAGFYAIRLGHLRKDLDTLRADNAKGELYLTDLVSHAGARGGATAIDAPFTEVSGINDRVDLAWVETEARKRINDAWMREGVTMVDPATTYIDAEVGPIGKDVWIGPNVALRGKTSIADGVRIDLGSVITDVTVAEHTNIKPHTIMTESTIGARVQLGPFSHCRPGTRLDEDVHLGNFVETKKAHLMAGAKANHLSYLGDASVGAKSNIGAGTITCNYDGVGKYKTTIEAGAFIGSNATLVAPVTIGRDAYTAAGSTVTKDVPRGALAISRPMQVNREGWADRFREVQDKRKKPDGT",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity to facilitate cell morphogenesis, lipid A biosynthetic process.2. A protein should be able to manipulate the activity of enzymes in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway, either by activating or inhibiting their function.3. The protein should have a high affinity for UDP-N-acetyl-alpha-D-glucosamine. The designed protein sequence is ",
    "response#1": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLSAVLDDPTGYGRILRSEDGSVVGIVEDRDASPEQRQIREVNTGILAAPTKYLREWLPQLRNDNAQGEYYITDVLALAVRDGVPVVASHPESEFEVMGVNSKVQLAELERVWQGDQARRLMLEGVSLIAPETVWFSSDTQVSSDVIIEPNVIFGPGVSIASGATIYGHSHIEGATIGANAKVGPFARLRPGAVMEEGSKVGNFVEMKKATLGKGAKANHLTYLGDAEVGEGANIGAGTITCNYDGYFKYKTVIGKGAFIGSNSALVAPVNIGDGAIVGAGATLTKDVAAGDLALVRAEQRIIEGWQRPVKQKK",
    "response#2": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLTNDPAEPGRYGRIIRNEAGQVERIVEFKDATDAERAISLCNSGVIAAKASDLFALLQKVGNDNAKGEYYLTDIVEIAVADGGKAVAVTADDALEVAGVNNRRQLSELEAIYQTRAREKLLA",
    "response#3": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLGFQANDPKGYGRIIRDGNNICGIVEEKDADANQRKINEVNSGIYCFNVEKLHEGLSRLSNNNVQGEYYLTDVVGIARSNGDKVIPVYLEDYWETLGVNSKVQLAQLEKIHQQRICDQWMLQGVTIIDPEATYIGTDVQIGQDVIIYPGTMITGPSIIANNVVIGANSQIINSTIGEHTTINASVISDSIIGASTTVGPFAHIRMHAEIGNKARIGNFVEIKKSVFKDGAKSAHLSYIGDAELGENVNMGCGSITVNYDGKNKHKTVIGANTMVGCNVNLVAPVTVEPNAYLAAGSTITKNVPEDALAIARSKQRNIEGWVEKKRPGTGAEEASKK"
  },
  {
    "reference": "MLIEKNILLGVSGGIAAYKACALTSQLTQRGANVKVMMTEHAAKFVSPLTFQALSRNPVYVDTFDEKDPEKIAHIDIADWADIALLAPATANIIGKIATGIADDMLSTTLLATQAEIYIAPAMNVNMYAHPAVQRNMQQLEEWGHHFIEPGAGYLACGYVGKGRLEEPESIIKAVENHQNRLKPLVGKNILISAGPTREVIDPVRFFSNRSSGKMGFALAEAAAEAGGDVTLVAGPCSLSIKNPAIKRIDIVSAQDMYEAMHHHFDTSDIVIKAAAVADYRPKVTYDNKMKKQPGEWHVEMERTKDILLSLGEKKTTQYLVGFAAETSMPIEYGIDKLVRKNLDSIIVNNVAEEGAGFEGDTNVVTYINKHQQIEELEMNTKAEVAKHILNLIYRDMKDEVN",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein should be able to manipulate the activity of enzymes in the coenzyme A biosynthesis pathway, either by activating or inhibiting their function.2. The protein should have a low Km value for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.3. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A.4. The designed protein should have FMN binding to facilitate coenzyme A biosynthetic process, pantothenate catabolic process. The designed protein sequence is ",
    "response#1": "MRITLGVTGGVAAYKAAELVRRLQQEGHSVQVVMTRAAREFVTPLTFAALSGQKVITDLFRDSSGSEANLESAIEHIAVAQRTDLLLVAPATADIMAKFARGVADDFLTTLYLACTAPVVVAPAMNVNMWNHTATQENVATLRARGVKIAQPDEGYLACGMTGPGRLAGLEKVVLGVSGGIAAYKACELLRRLTESGHDVRVVPTDSALNFVGAATWSALSGNPVSTGVWESVHEVPHVRIGQAADLVVVAPATADMLAKAAHGLADDLLTNTLLTARCPVVFAPAMHTEMWEHPATQENVATLRRRGAVVIEPAVGRLTGVDTGKGRLPDPAEIFEVCRAVASGGRRDLDGETILVSAGPTCEDLDPVRFLTNRSSGKMGYAVAEAAARRGAKVILVSGPVNLETPAGVERADVRTAEEMRRAVLDRSAEASMIILAAAVADYRPVERRAEKIKKGNAPLTISLEPTTDILAELAKNKGQKIVVGFAAETERVAENARKKLASKNADLIVANDVTAEGAGFDQDTNVVTLVTGAGEEAIEPGPKDLLADRILDVVVAAR",
    "response#2": "MAGRRVMVTLGPTREAWDGVRFWSNPSSGLMGAALAVCAWLRGAEVTAVCGPGVRARMPQGIARRDVVSARDMFAVAANLWPQMDVGMFTAAVADFSPKPMGGRKFKKSEAPQGFSLDFQPNPDILQSLAEQRAPGQKILAFAAETAPDMHALLPLAHAKLARKKADLLAGNLVNAADSGFGSPTNSMAAAECCLHGRSVVITAGPTREAIDPVRYLSNYSSGKMGFALAAAAVKAGASVTLVAGPCQLATPAHVTRVDVVSALDMQQAVSEHIAHCDIFIGCAAVADFRPVQVAQQKMKKQQDHDHMAIELVKNPDILAWVATQSPTPFTVGFAAETNEVAKYARDKLERKGIQLIVANDVSDATIGFNSDNNAVTVFWPGGKVELPQASKQVLARQLIAHIAKRLEEG",
    "response#3": "MAGRRVMVTLGPTREAWDGVRFWSNPSSGLMGAALAVCAWLRGAEVTAVCGPGVRARMPQGIARRDVVSARDMFAVAANLWPQMDVGMFTAAVADFSPKPMGGRKFKKSEAPQGFSLDFQPNPDILQSLAEQRAPGQKILAFAAETAPDMHALLPLAHAKLARKKADLLAGNLVNAADSGFGSPTNSMAAAASATS"
  },
  {
    "reference": "MAIVDVKVPQLSESVAEATMLNWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLSIIVKNDGDTVVADEIIAKIDTEATAGAAAPAAAAPAPAAAAPAPAAAVAAPAAAGGVAMPSAAKLMAEAGLSAGQVAGTGKDGRITKGDALAAAAAPAAKAAPAPAAAKPALQQVSAPVDFAALGDRPEERVPMSRLRARIAERLLQSQSTNAILTTFNEVNMKPVMDLRNKYKDRFEKEHGVKLGFMSFFVKAAVHALKKFPLINASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSLADIEKKIAEFGVKARDGKLSLEELTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKDRPVVEDGQIVIRPMNYLAMSYDHRIIDGREAVLGLVAMKDALEDPARLLLDL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that can perform dihydrolipoyllysine-residue succinyltransferase activity in oxoglutarate dehydrogenase complex to support L-lysine catabolic process to acetyl-CoA via saccharopine.2. The designed protein have Peripheral subunit-binding (PSBD), Lipoyl-binding domains with specific sequence motifs that are crucial for its activity.3. A protein should be able to manipulate the activity of enzymes in the Amino-acid degradation, glutaryl-CoA from L-lysine: step 6/6 pathway, either by activating or inhibiting their function.4. The protein must exhibit the following characteristics: E2 component of the 2-oxoglutarate dehydrogenase (OGDH) complex which catalyzes the second step in the conversion of 2- oxoglutarate to succinyl-CoA and CO(2)..5. The protein design should incorporate a binding pocket that specifically recognizes (R)-lipoate to enhance enzymatic efficiency.6. The protein's active site must be carefully engineered to ensure that the amino acids involved in (R)-N(6)-dihydrolipoyl-L-lysyl-[2-oxoglutarate dehydrogenase   complex component E2] + succinyl-CoA = (R)-N(6)-(S(8)-   succinyldihydrolipoyl)-L-lysyl-[2-oxoglutarate dehydrogenase complex   component E2] + CoA are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "LDWATAEALALGSLLAQGFNVRLSGQDVGRGTFSQRHAIVVCQETDDTYIPLNHMDPNQKGFLEVSNSPLSEEAVLGFEYGMSIESPKLLPLWEAQFGDFFNGAQIIFDTFISGGEAKWLLQSGIVILLPHGYDGAGPDHSSCRIERFLQMCDSAEEGVDGDTVNMFVVHPTTPAQYFHLLRRQMVRNFRKPLIVASPKDVQVLRGAAAAVVKNMSASLDVPTATSVRAIPAKLLIDNRIVINNQLKRNRGGKISFTHLLGYALVQAVKKFPNMNRHYAEVDGKPTAVTPAHTNLGLAIDLQGKDGKRSLVVAGIKRCETMRFAEFVSAYEDIVRRARDGKLTAEDFSGVTISLTNPGTIGTVHSVPRLMAGQGAIIGVGAMEYPAEFQGASEERIAELGIGKLITLTSTYDHRIIQGAESGDFLRTVHEMENRIKMTRLRQKIAERLLETKNNTAMLTTFNEVNMQPIISLRKKYGEFFEKKHGVRIGFMPFFVKAVVESLKKFPEINASIDKNDIVYYKNIDVSIAVSTPRGVITPVLRNADNMSMADIEKKIKEFSIKGIENKIKIEELIGGNFTITNGGIFGSLMSTPIINPPQSAILGMHLIKERPMAINGKVKILPMMYLALSYDHRLIDGKESVSFLVTIKNILEDFNRIELQTLRGAAAAVVKNMSASLEVPTATSVRAIPAKLLIDNRIVINNQLKRTRGGKISFTHLLGYALVQAIKKFPNMNRHYAEIDGKPTAITPAHTNLGLAIDLQGKDGKRSLVVAGIKNCETMRFAQFVTAYEDIVRRARDGKLTAEDFAGVTISLTNPGTIGTVHSVPRLMNGQGAIIGVGAMEYPAEFQGASEERIAELGIGKLITLTSTYDHRIIQGAESGDFLRTIHEM",
    "response#2": "DSMVPLLDELVRQAIEHEIDAVNIGMAHRGRLNVLAHVLGKPYEMIFAEFQHAESKNFIPSEGSVAITYGWTGDVKYHLGAARRLRNQSAHTMRITLANNPSHLEVVNPVVLGYTRAAQEDRTKPGVPVQNTDASFAILIHGDAAFPGQGIVAETLNLSQLRGYTTGGTIHIIANNMIGFTTESYDSRSTTYASDMAKGFEVPIVHVNADDPEACLAAACLAFAYRQRFKKDFVIDLIGYRRFGHNEMDEPMATNPTMYAIINQHPTVRKLYAQKLMEKGIITEREVDEMEQEVAERLKIAYERVPKGKAGECDMDWGAAETLAYATILNEGISCRISGEDVSRGTFFHRHAFIHNQINGSIYIPLNNISKKQGKFQIWDSVLSEEAVLAFEYGYSLSSPNTLTIWEAQFGDFINGAQIVIDQFISSGEQKWNKKSNLVVLLPHGYEGQGPEHSSSRIERFLQLCAEENMQICIPTTSSQIFHIFRKQIFDKILKPLIIFTPKSLLT",
    "response#3": "FDWGAAETLAYATILNEGISCRISGEDVSRGTFFHRHAFIHNQINGSIYIPLNNISKKQGKFQIWDSVLSEEAVLAFEYGYSLSSPNTLTIWEAQFGDFINGAQIVIDQFISSGEQKWNKKSNLVVLLPHGYEGQGPEHSSSRIERFLQLCAEENMQICIPTTSSQIFHIFRKQIFDKILKPLIIFTPKSLLRPDETQVLRGAAAAVVKNMNISLEVPTATSVRAIPAKAMIDNRIVINNHLKRTRGGKISFTHLLGYAIVQGVKKFPNMNRHFAEIDGKPNAVTPAHTNLGLAIDLQGKNGSRSLVVAAIKGCETMKFGQFIAAYEDIVRRARDGKLTAEDFAGVTISLTNPGTLGTVHSVPRLMSGQGAIIGAGAMEYPAEFQGASEERIAELGMGKLITLTSTYDHRIIQGAESGDFLRTVHQLDVQVLRGAAAAVVKNMSASLDVPTATSVRAIPAKLLIDNRIVINNQLKRNRGGKISFTHLLGYALVQAVKKFPNMNRHYAEVDGKPTAVTPAHTNLGLAIDLQGKDGKRSLVVAGIKRCETMRFAEFVSAYEDIVRRARDGKLTAEDFSGVTISLTNPGTIGTVHSVPRLMAGQGAIIGVGAMEYPAEFQGASEERIAELGIGKLITLTSTYDHRIIQGAESGDFLRTVHEMELQTLRGAAAAVVKNMSASLEVPTATSVRAIPAKLLIDNRIVINNQLKRTRGGKISFTHLLGYALVQAIKKFPNMNRHYAEIDGKPTAITPAHTNLGLAIDLQGKDGKRSLVVAGIKNCETMRFAQFVTAYEDIVRRARDGKLTAEDFAGVTISLTNPGTIGTVHSVPRLMNGQGAIIGVGAMEYPAEFQGASEERIAELGIGKLITLTSTYDHRIIQGAESGDFLRTIHEM"
  },
  {
    "reference": "MQKTKMIFTIGPASXTEEMLVXFI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be able to perform pyruvate kinase activity, ATP binding, kinase activity under specific conditions.2. Ideal protein should have a binding affinity for key enzymes in the glycolysis, pyruvate from D- glyceraldehyde 3-phosphate: step 5/5, Carbohydrate degradation pathway to enhance the overall efficiency of the pathway.3. The protein must be engineered to have a high affinity for K(+) in order to increase its catalytic activity.4. The protein should be designed to have high stability and activity towards ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate. The designed protein sequence is ",
    "response#1": "MRKTKIVCTIGPASESEEMLEKLIKAGMNVARLNFSHGDQAEHKARIDTIRKVSKRLGKTVAILLDTKGPEIRTHNMKDGLIELEKGSEVTVSMTEVEGTPEKFSVTYENLINDVEEGSYILLDDGLIELQVKSIDKANGEVLCDVLNTGELKNKKGVNLPGVKVSLPGITDKDADDINFGISEGVDFIAASFVRRPSDVLDIRKLLEAKQNKNISIIPKIENQEGIDNIKEILEVSDGLMVARGDMGVEIPPESVPMVQKDLIRQCNKLGKPVITATQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGQYPEEALMRGDIERVVALTEEAIGESVVRTAQELGVKTIIAATSSGYTARMISKYRPDATIVALTFDEKIQHSLGIVWGVEPVLAKKPSNTDEMFEEAARVAKEHGFVKDGDLVIIVAGVPFGQSGTTNLMI",
    "response#2": "MRKTKIVCTIGPASESPELLEQLIEAGMNVARLNFSHGNHAEHKARIDSIRKVAREKGKVVGILLDTKGPEIRTHSMMNGKLELVTGQKIDISMTQVEGNNDVFSVSYDKLIEDVNEGSVILLDDGLIQLEVTGKDVARGLIHTLIINSGSLSNNKGVNIPGVSVQLPGMTEKDAEDILFGIREGVDFIAASFVRRASDVMEIRALLENNNGSNLQIIPKIENQEGVDNIDEILNVSDGLMVARGDLGVEIPPEEVPLVQKNLIEKCNQAGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGIYPVESLIELADTIF",
    "response#3": "MRKTKIVCTIGPASESEEMLEKLIKAGMNVARLNFSHGDQAEHKARIDTIRKVSKRLGKTVAILLDTKGPEIRTHNMKDGLIELEKGSEVTVSMTEVEGTPEKFSVTYENLINDVEEGSYILLDDGLIELQVKSIDKANGEVLCDVLNTGELKNKKGVNLPGVKVSLPGITDKDADDINFGISEGVDFIAASFVRRPSDVLDIRKLLEAKQNKNISIIPKIENQEGIDNIKEILEVSDGLMVARGDMGVEIPPESVPMVQKDLIRQCNKLGKPVITATQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGQYPEEALQFISEFEG"
  },
  {
    "reference": "MAKVLLIVFIMVYAGSCAIWVSQPPEIRAQEGTTASLPCSFNASRGKAAIGSATWYQDKVAPGMELSNVTPGFRGRVASFSASQFIRGHKAGLLIQDIQSHDARIYVCRVEVLGLGVGTGNGTRLVVEKEPPQQASNAEPERAAYTSLLLRAGVYALSFLSVATGSVIYYQGKCLCHVGNTATPPTASEERF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. The designed protein should contain one or more Ig-like domains that are essential for its function.3. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.4. For general function, the protein need meet that Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG that promote DC maturation.; It controls, for instance, NK cells cytotoxicity against tumor cells; Cell membrane receptor of natural killer/NK cells that is activated by binding of extracellular ligands including BAG6 and NCR3LG1; Stimulates NK cells cytotoxicity toward neighboring cells producing these ligands5. Design the protein with a signal peptide incorporated.6. The ideal protein would be one that plays a critical role in natural killer cell activation, positive regulation of natural killer cell mediated cytotoxicity through identical protein binding within plasma membrane. The designed protein sequence is ",
    "response#1": "MDPKGLLSLTFVLFLSLAFGASYGTGGRMMNCPKILRQLGSKVLLPLTYERINKSMNKSIHIVVTMAKSLENSVENKIVSLDPSEAGPPRYLGDRYKFYLENLTLGIRESRKEDEGWYLMTLEKEKQEVTFNLDPVPVARCVSAGGRPPARVSWLSPLDGEAKETQGSGPLPGTVTVTSRYTVTPLSEADGVKVICKVEHESFQEK",
    "response#2": "ARSECKRSHKFVPGAWLAGEGVDVTSLRRSGSFPVDTQRFLRPDGTCTLCENALQEGTLQRLPLALTNWRAQGSGCQRHVTRAKVSSTEAVARDAARSIRNDWKVGLDVTPKPTSNVHVSVAGSHSQAANFAAQKTHQDQYSFSTDTVECRFYSFHVVHTPPLHPDFKRALGDLPHHFNASTQPAYLRLISNYGTHFIRAVELGGRISALTALRTCELALEGLTDNEVEDCLTVEAQVNIGIHGSISAEAKACEEKKKKHKMTASFHQTYRERHSEVVGGHHTSINDLLFGIQAGPEQYSAWVNSLPGSPGLVDYTLEPLHVLLDSQDPRREALRRALSQYLTDRARWHRAMVPARPRSHCQYSFSTDTVECRFYSFHVVHTPPLHPDFKRALGDLPHHFNASTQPAYLRLISNYGTHFIRAVELGGRISALTALRTCELALEGLTDNEVEDCLTVEAQVNIGIHGSISAEAKACEEKKKKHKMTASFHQTYRERHSEVVGGHHTSINDLLFGIQAGPEQYSAWVNSLPGSPGLVDYTLEPLHVLLDSQDPRREALRRALSQYLSPRDPCQCVCHGSAVTTQDCCPRQRGLAQLEVTFIQAWGLWGDWFTATDAYVKLFFGGQELRTSTVWDNNNPIWSVRLDFGDVLLATGGPLRLQVWDQDSGRDDDLLGTCDQAPKSGSHEVRCNLNPAVFRYNVSGSNGTCLLASMGLQLNVTYRRVDNKTVTREFNVNPNKTTFGGNCSATLATLELHSENLLLLALQFVMNESSSRVFLQGVQLNLTLPDAKEGSFTATNSSLRALQATAGNSYKCNAEQRLRVTSSFSLNMFRVWLQAFR",
    "response#3": "MDPKGLLSLTFVLFLSLAFGASYGTGGRMMNCPKILRQLGSKVLLPLTYERINKSMNKSIHIVVTMAKSLENSVENKIVSLDPSEAGPPRYLGDRYKFYLENLTLGIRESRKEDEGWYLMTLEKK"
  },
  {
    "reference": "MSLPIVFVTGNAKKLEEFIAILGKEFPRRITSKKIDLPEYQGEVDDICRDKCRAAANLVKGPVIIEDTCLCFNALKGLPGPYIKWFLEKLGPEGLHRMLYGFEDKSAEAVCTFGYCSGEDSEVHLFQGRTQGTIVSPRGSRDFGWDPCFQPLDYDKTYAELLKEEKNKISHRIFLLCLTAAYAVPAAEKTVIYNYYADVKAGTIEPAQYVSQFALTGQLHVKKDTSDPTLNNAYYIKLNHLKYGMYNGMVIHHQPVKVLHELGEAAQQIQEPFVIVYDESGKFQGIKMPETESGWSRNIKQGIASMLQLDLAHIQMQTPMKPHGFITHENTIHGTCQVAYDVHLKNLTNSENTNVFVVTKMHDLRNCSHFVQRAFDYFECEKCHMESVDDMATFARRVFEIKRQDNNILIERLVAHSTINYFPYNSRSESHYLLTNTTLDLHSVVPTIEVPVLAVNVQNVPIIRDITFNKPLGNYALHAAEDLTHGRHIVKLDARIPKLKKMLVEATDYLEENHLEMKEPEWKHGQTINRLQHTMSYMDLGSLEQVYNAIQDSKTPKEDTMRNIFLRMIPTVGTTATCHFVRNVVRKHKVSDNTAVDMLRKLPMHVKVPSERLLLEMEDLLKLGSTVSPQVRKASILCFSILIRKTFIHQQSDIVNPLLERYLHRFFDHVKNEQSYEMKVIYLMAMKNVQVGNIEKLLEPIIRGEVIISEHPYHIRAQAIWAIKKAVSDRVEYTHNLLWPILADVTQPLSIRIVAYETLMSQMPDMQR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The Mg(2+), ITP binding site should be stable and able to withstand changes in environmental conditions.2. The protein must exhibit the following characteristics: Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives; The enzyme does not distinguish between the deoxy- and ribose forms.3. The protein design must be able to perform metal ion binding, lipid transporter activity, dITP diphosphatase activity in order to contribute to nucleotide metabolic process.4. The presence of Mn(2+) is necessary for the protein to function in the desired environment.5. The protein should be designed to catalyze the following reaction dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MNEELIVLASGNAGKVRELQALLGDAGFTVRPQSEFGVSDVRETGVTFVENALIKARHAAAASGLPALADDSGLVVDALGGAPGIYSARYAGEPKSDARNNEKLIADLAGVPDEQRTARFHCVLVYLRHADDPTPIICHGVWPGRIAEAAGGSGGFGYDPLFYLPQLGCTAAELSAEDKNRISHRGQALRAMIAHLKH",
    "response#2": "MKPLILASNNAGKLREFAQLLGGMGWNVVPQSELSIGDAEEPHVTFVENALAKARHAARVSGLPALADDSGICVEALDGAPGVYSARYAGEPKSDARNNAKLLADLEGQADRHAHYYCVIALVRHADDPQPIIAQGRWHGEILAEPRGAGGFGYDPLFWLPALNKSAAELDAAQKNRLSHRGQALAQLLAELRS",
    "response#3": "MNKYVLASKNEGKVKELKAMLGE"
  },
  {
    "reference": "MTSLNLNFRVSVVLVILLSIAFIFINSGFDLEYIIPRRLIKLSAIIIGGSCVAISAVIFQALARNRILTPSIMGYESIYLVWQALLLLFVGTSGSAVLGVVGNFVVSAVLILLYSFVIQFWVLKRFQHDMHQVLLIGFVLTMVLTTVAQFIQIRISPGEFSIFQGLSYTSFERAKPSTLLFAGTVLSILALFANKWVSELDVIGLGRDQAMSLGLNDAHYIPKYFSVIAILVAISTSLIGPTAFMGVFIANIAYSITGSPQYRHTLPVACTIAIVMFLTAQLMVEHFFNYKTTVSILVNVLCGGYFLIITMRARSQL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. For general function, the protein need meet that Responsible for the translocation of ferric- anguibactin across the cytoplasmic membrane.; Involved in the uptake of iron in complex with the siderophore anguibactin3. A protein with transmembrane transporter activity is needed to enhance ion transport, iron ion homeostasis in a specific context. The designed protein sequence is ",
    "response#1": "NRGLSIASGVDVPSPPSSDTLLSPSWAYNDSEDKGMMIRAELDLSNSVTAYGAVGASRTDFDSNVPQRVKIIDDSGTLEVSLGSVKLESKRTSGEVGIRSSFDTGPIEHYLVLNSTYFREDKNDSPTGNNNPGSWNPNIYNPVWGPEDSTYDNYYELPVDSTQISFGVADTLSLANGKYQVTLGLRHQSIDYESGVTWNGNAFPTTKLKESTYTPAIVALYKVSDSVSLYGNYTEGLTNGKTAGSGAANVGEAFEPQKTKQTEAGLKLDMNDFAHTFSLFEIKKPNGYQDPDTNIYSFGGEQRNRGIEWGFYGTVLEDYTLTGGIAYTDAEITKATDVTTEGKQATKLPDLQAKLALEWNLPVMRQLTLIGQANYMSEQYIDAQNTQSLSAQTIFDLGARYNSTIANQSVIWRLAVNNM",
    "response#2": "NSRRTAIVGPSGSGKTTLLRLIAGFETPDTGVITLNGQTLCDNGFSVPAHQRRIGYVPQDGALFPHLCIADNIAFGLKGSKAEKQKRVDELMALVSLPTHLQKHSPHEISGGQQQRVALARALAQRPALMLLDEPFSALDTGLRAATRKAVMDVLQQANVASILVTHDQGEALSFADRVVVMREGS",
    "response#3": "NRGLSIASGVDVPSPPSSDTLLSPSWAYNDSEDKGMMIRAELDLSNSVTAYGAVGASRTDFDSNVPQRVKIIDDSGTLEVSLGSVKLESKRTSGEVGIRSSFDTGPIEHYLVLNSTYFREDKNDSPTGNNNPGSWNPNIYNPVWGPEDSTYDNYYELPVDSTQISFGVADTLSLANGKYQVTLGLRHQSIDYESGVTWNGNAFPTTKLKESTYTPAIVALYKVSDSVSLYGNYTEGLTNGKTAGSGAANVGEAFEPQKTKQTEAGLKLDMNDFAHTFSLFEIKKPNGYQDPDTNIYSFGGEQRNRGIEWGFYGTVLEDYTLTGGIAYTDAEITKATDVTTEGKQATKLPDLQAKLALEWNLPVMRQLTLIGQANYMSEQYIDAQNTQSLSAQTIFDLGARYNSTIANQSVIWRLAVNNV"
  },
  {
    "reference": "MTKYIFVTGGVVSSLGKGIVAASLGRLLKNRGLKVTMQKFDPYINVDPGTMNPYQHGEVFVTNDGAETDLDLGHYERFVDNDLNRYSNVTTGKVYSEVIRRERRGDYNGATVQVIPHITDMIKDRIMRAANTTDSDVIITEVGGTVGDIESLPYLEALRQMKAEVGAENVIYIHATLIPYLRAAGEMKTKPTQHSVKELRSLGIQPNILVVRTEQPITQEMRNKIANFCDVDPEAVIESLDVKTLYSIPLNLQKQNMDQIVCDYLHLDTPKADMTEWQQLEHRVQNLTHKTKITLIGKYVELQDAYISVAEALKHAGYVFDSDIELVKVQAEDITDDNVADYLGDTQGIIVPGGFGNRGLEGKIATIKYARENNIPFLGICLGMQMACVEFARDVLGYADADTTEVNPDTKHNIIDLMADQEGVENIGGTLRLGAYPCKLKPGSVAAAAYDNQSVIQERHRHRYEFNNKYRDEMAAKGFVFSGVSPDNRLVEIVELPEKKFFVAAQYHPEFLSRPMRPEGLFKAFVKAASDLPEHPWEKAEK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The presence of Glutamine amidotransferase, CTP synthase N-terminal domains is critical for the interaction of the designed protein with its intended targets.2. The designed protein should have a high specificity for L-glutamine, ATP, UTP binding, with minimal off-target effects.3. A protein with metal ion binding, CTP synthase activity, ATP binding, glutaminase activity functions is required to enable glutamine metabolic process.4. The active site, Nucleophile; for glutamine hydrolysis active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.5. The protein design should take into account the physiological conditions of the CTP biosynthesis via de novo pathway, CTP from UDP: step 2/2, Pyrimidine metabolism pathway. The designed protein sequence is ",
    "response#1": "VTIAFVGKYLDLKESYKSLTEALIHAGAHINAKINIHWCDSERIEDAGAYEVIQTCDAVLVAGGFGHRGVEGKLKAIEYARTNNIPYLGICLGMQLAVIEYARNVLGIEDANSVEFDPEAKNPLIYLIDEFLDQSGNKQVRTHESPMGGTMRLGEYPFTPKKGSKLQKAYGDKETYYERHRHRYEANPKYKEALEKAGMIISGESHGLIEAVELKDHKWFVGVQFHPEFTSHLETPNPIILEFIQACVAQ",
    "response#2": "RFIFVTGGVVSSLGKGLAGASVAALLQARGYKVRMRKLDPYINVDPGTMNPYEHGECYVTDDGAETDLDLGHYERFTGVSSKQSDNVTTGRIYQTVIRKERRGDYLGATVQVIPHITNAIKEFATADIEDEDFVLIEIGGTVGDIESLPFLEAIRQLGNELGQDAIFMHLTLVPYIAAAGELKTKPTQHSVKELQSIGIQPNILLCRSEHPIPAGLLAKIGQFCNVPEKAVIPALDVRTIYEVPLSYHKEGLDTAVLDAFGMWTSAPDLSAWERIVRRVRNPEGEVTIGLVGKYIDLPDAYLSVTEALKAGGFAHHAKVSIRWVASDECETPEGAARNLADLDGMLIPGGFGVRGIQGKLGALKFARENGIPTLGLCLGLQCMVIEYARDIVGLAGASSSEFDPDTEFPVIATMAEQIAIIEGGDLGGTMRLGLYEAALKPGSIVASLYGSEHAAERHRHRYEVNNAYRQQIADAGLVFSGISPDRTLVEFVELPRDVHPFYVGTQAHPELRSRPNHAHPLFRGLVGAAIDQAQEARLVELPARLPDAYLSVTEALRAGGFANDAKVVIRWVTSDDCQTMEGAEDALGGVDAVLVPGGFGVRGIEGKLGALRWAREKQVPTLGICLGLQSMVIEYARNVLGIADASSTEFDPDTEHPLIATMEEQLAFVEGQGDLGGTMRLGAYDAALTPGSVAATAYGSTLVSERHRHRYEVNNQYREQLEAAGLVISGTSPDGSLVEFVELPRDVHPYYVSTQAHPEFKSRPTHAHPLFAGLVEGRGAR",
    "response#3": "RFIFVTGGVVSSLGKGLAGASVAALLQARGYKVRMRKLDPYINVDPGTMNPYEHGECYVTDDGAETDLDLGHYERFTGVSSKQSDNVTTGRIYQTVIRKERRGDYLGATVQVIPHITNAIKEFATADIEDEDFVLIEIGGTVGDIESLPFLEAIRQLGNELGQDAIFMHLTLVPYIAAAGELKTKPTQHSVKELQSIGIQPNILLCRSEHPIPAGLLAKIGQFCNVPEKAVIPALDVRTIYEVPLSYHKEGLDTAVLDAFGIDAPVVDLGEWEDVVERRRNPTGEVTIGLVGKYIDLPDAYLSVTEALKAGGFAHHAKVSIRWVASDECETPEGAARNLADLDGMLIPGGFGVRGIQGKLGALKFARENGIPTLGLCLGLQCMVIEYARDIVGLAGASSSEFDPDTEFPVIATMAEQIAIIEGGDLGGTMRLGLYEAALKPGSIVASLYGSEHAAERHRHRYEVNNAYRQQIADAGLVFSGISPDRTLVEFVELPRDVHPFYVGTQAHPELRSRPNHAHPLFRGLIAASLDNA"
  },
  {
    "reference": "MKLYKVLNSKTTDKSLCLEVEIDPNYWQATQKKLVGEMAKSIKIKGFRPGKIPPNLASQSINKAELMQKSAQNVMNSIYESVQQEEIVASNDNVIDDYPTIDFKTITEQNCVLLFYFDLIPNFQLPDYKKIKDLTPLTKLTEAEFNNEIEKLAKTKSTMVDVSDKKLANGDIAIIDFTGIVDNKKLASASAQNYELTIGSNSFIKGFETGLIAMKVNQKKTLALTFPSDYHVKELQSKPVTFEVVLKAIKKLEFTPMDETNFKSFLPEQFQSFTSLKAFKSYFHKLMENKKQETILQENNQKIRQFLLTNTKLPFLPEALIKLEANRLLKLQQSQAEQYKIPFEKLLSASNITLTELQDRNIKEAKENVTFALVMKKIADIEKIKVDNNKIKAEIENVIAVEYPFASDEMKKQLFFNMEQQKEFVESIIINRLTTTKIVSYSTH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must possess ribosome binding, peptidyl-prolyl cis-trans isomerase activity, protein folding chaperone to support cell cycle, protein unfolding under certain conditions.2. For general function, the protein need meet that Acts as a chaperone by maintaining the newly synthesized protein in an open conformation; Functions as a peptidyl-prolyl cis-trans isomerase .3. The target protein must exhibit strand-strand-helix-strand-strand-helix-strand as its primary conformation. The designed protein sequence is ",
    "response#1": "FDIEINREPVGRIMFQLFSDICPKTCKNFLCLCSGEKGLGKTTGKKLCYKGSTFHRVVKNFMIQGGDFSEGNGKGGESIYGGYFKDENFILKHDRAFLLSMANRGKHTNGSQFFITTKPAPHLDGVHVVFGLVISGFEVIEQIENLKTDAASRPYADVRVIDCGD",
    "response#2": "LDEAKEDIKKRMDRNQQEHANQMNTMLLTQFANEDCQFSHIPKSLLDREVNLLYQQFANQMQQIKMSVEDALKIQNQTKEQLYQRLTEQATRAIKLVLVLEEIGDLEKISVSEAEINQEIEERIKEISNNQELPKEQYDAIKNYFVTQKELIESMLTNKKVVDLIK",
    "response#3": "GNITLELFYKDAPQAVSNFVTLAKEGFYNGLNFHRVIAGFVAQGGCPYGTGTGGPEHRIKCEVAHNPNKHQRGSISMAHAGRDTGGSQFFLCFVDLPHLDGEHTVFGKITSAESLSVLDKIKQGDIIESVVFSPSS"
  },
  {
    "reference": "MQKEKLFGKTLNELIAVTKRVGLPGFAAKQIADWLYRKEIQTIDEMTNLSKKTRELLSNDYETGLAGPVSFLESIDGTKKYLYKVLDNKFIETAYIPDDDRATICVSSQAGCKMGCLFCMTGKQGFQGNLSSNEILNQFRSLPEFKQLTNMVYMGMGEPLDNLAEVLKSLDVLTSGWGYGWSPSRITVSTIGIVDNIKEFLEGTRCHLSVSLHSPFDEERRKLMPVRKIYSINEVLGVIRNFDFSNQRRVTFEYILFDGLNDSPRHIKELARILNGIKCRINLIRFHPIPGSSLRSPDDRAMVVFKEALNAKGILTTIRVSRGVDIKAACGLLSTLEQNKA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must be able to 4 iron, 4 sulfur cluster binding, metal ion binding.2. The designed protein must have a Proton acceptor, S-methylcysteine intermediate that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "SSQVGCTLNCRFCYTGTMPMVRNLTPGEILGQFMLARDCLNDWINCDGRRKISTIVMMGMGEPLLNYDNVARALTIIMDGDGISLSRRGITLSTAGLVPAIQRCGRELRVNLAVSLHAVRDEVRDVLVPLNRRYPIAELIQACRQYPSANNARRITFEYVMLENVNDSAKDAHR",
    "response#2": "MILGIDVGSTTTKMVLMEDSKIIWYKIEDIGVVIEEDILLKMVKEIEQKYPIDKIVATGYGRHKVSFADKIVPEVIALGKGANYFFNEADGVIDIGGQDTKVLKIDKNGKVVDFILSDKCAAGTGKFLEKALDILKIDKNEINKYKSDNIAKISSMCAVFAESEIISLLSKKVPKEGILMGVYESIINRVIPMTNRLKIQNIVFSGGVAKNKVLVEMFEKKLNKKLLIPKEPQIVCCVGAILF",
    "response#3": "MILGIDVGSTTTKMVLMEDSKIIWYKIEDIGVVIEEDILLKMVKEIEQKYPIDKIVATGYGRHKVSFADKIVPEVIALGKGANYFFNEADGVIDIGGQDTKVLKIDKNGKVVDFILSDKCAAGTGKFLEKALDILKIDKNEINKYKSDNIAKISSMCAVFAESEIISLLSKKVPKEGILMGVYESIINRVIPMTNRLKIQNIVFSGGVAKNKVLVEMFEKKLNKKLLIPKEPQIVCCVGAILLF"
  },
  {
    "reference": "MLKGKNVILGVTGGIAAYKIVGLASALVKQGASVNVLMTEAATHFITQDTFEAITKNKAIVDTFDRNFEYEVEHIALAKKADIFMVAPATANTIAKLAHGIADNMLTTTFLACTCPKMVAPAMNTEMYNNEVTQENMDKLESMGVTVIPADSGRLACGDTGIGKMPEPELLLAYIEKEIAREKDMEGMHVLVTAGATKEPLDPVRFITNHSTGKMGFAIAKECMLRGAKVVLCKAATSSTPPEFVDIIDTPTSAEMFEACKRYARGADFIIKAAAVSDYRPAEVAENKMKKKDGELSINLERTVDILKYLGEHKSPSQVICGFSMETENLIENSTAKLHKKNADLIVANNLKDSGAGFGTDTNLVTFITKEGYKSLELLSKADVARQIVDKLLEISSAKNDAMPLGE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must have high specificity towards metal ion binding, phosphopantothenate--cysteine ligase activity, FMN binding.2. The protein should be designed to catalyze the following reaction H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.3. Ideal protein should have a binding affinity for key enzymes in the Cofactor biosynthesis pathway to enhance the overall efficiency of the pathway.4. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.5. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.6. The presence of Flavoprotein domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MNILLGVSGSIAAYKSPDLVRRLKEVGFDVRVVMTDGGRSFVTPLTLQAVSGYPVVYDLLDPAHEAGMGHIELARWADLILIAPTSANMIAKLAGGFADDLLSAICLATEKPIMLAPGMNRLMWANQAVQENVAKLEARGMILLAPESGSQACGEIGQGRMPEPLQIRDAEGILQAIKGKKITITAGPTREALDPVRFISNHSSGKMGFAIAQAAAQRGAEVTLIAGPVTLPTPACVKRIDVESAQEMYQQVMDIVPSQDIFIGCAAVADYRAKQVAPEKIKKQGDEVTITMIKNPDIVASVGKMVEHRPFVVGFAAETQNVEEYARRKREQKQLDLICANDVSLKDAGFNSDNNALHLIWKNGEISLEKMSKEELANSLLDEVIKIYSES",
    "response#2": "MNILLGVSGSIAAYKSPDLVRRLKEVGFDVRVVMTDGGRSFVTPLTLQAVSGYPVVYDLLDPAHEAGMGHIELARWADLILIAPTSANMIAKLAGGFADDLLSAICLATEKPIMLAPGMNRLMWANQAVQENVAKLEARGMILLAPESGSQACGEIGQGRMPEPLQIRDAEGILAAVNNIVVGVTGSIACFKVAGWVSTLAKEEALVEVIMSRAAQQFVAPLTFSSLSGRRVYTDMFTDDRDGAINHIGLAREADGILVAPATAQTIARLAHGLADDLLAATVLAATVPVIVCPAMNVKMYEHPATRRNLQTLREFGYTVVEPEIGAMACGEFGNGRLPEWEVVAEYLLRLASGCSRPLAGQVVLVTAGPTREPIDPARFLSNRSSGKMGYALARTAFRRGARVILVSGPTTLDGPPGVQRIDVTTAQQMYDAVMAHYREATVIIKAAAVADFRSREESTEKIKKDQASLVLHLQQNPDILKELGARCDHDRQLLVGFAAESARFAEEGRKKLHNKQCDLIAVNDIGSERTGFASDANRLLDLLYPDGSREELPLLSKYEMSRTLFSIVQPFLE",
    "response#3": "MNILLGVSGSIAAYKSPDLVRRLKEVGFDVRVVMTDGGRSFVTPLTLQAVSGYPVVYDLLDPAHEAGMGHIELARWADLILIAPTSANMIAKLAGGFADDLLSAICLATEKPIMLAPGMNRLMWANQAVQENVAKLEARGMILLAPESGSQACGEIGQGRMPEPLQIRDPEGIRACIGENIVVGVTGSIACFKVAGWVSTLAKEEALVEVIMSRAAQQFVAPLTFSSLSGRRVYTDMFTDDRDGAINHIGLAREADGILVAPATAQTIARLAHGLADDLLAATVLAATVPVIVCPAMNVKMYEHPATRRNLQTLREFGYTVVEPEIGAMACGEFGNGRLPEWEVVAEYLLRLAAQADRPSRLAGLRLVVSAGGTREPLDPVRYLGNRSSGKQGYALARTAVARGARVTLIAANAGLPDVAGADMVHVQTAVQLREAVLAAAADADAVVMAAAVADFRPAVYASGKIKKVDGRDPEPVALVRNPDILAELSATRPKAGQLVVGFAAETDDVLANGRKKLARKGCDLLVVNEVGDTKAFGSEENEAVVLGADGSETPVPHGPKEALADVVWDLVAARL"
  },
  {
    "reference": "MLLATDLDGTFLGGDPKDRLSLYQTIAAHPEIRLAYVTGRSLESVLPLLADPTLPQPDFIISDVGASLVHGDSLQPIQPLQSAVDALWPGDSQVASAIERFALERQDVPQVRRCSYFCNPEQAANPELLTIAESLGCDLLYSAERYLDFLPKGVNKGSSLQALIDWLELDNDQVLTAGDTLNDLSMLSGQFKGVCVGASEPGLLQATRQYSLVFHAERAGCGGILEAFVHFGFLGKQGIAAEGKQVAEPGKADLMMVYHRLPYEEFRGADGKLQRRRPTSPNGIIPTLLSFFGDGRAGSWVAWAEHDENSGETFDSHTTVDAERYPKLTAARVALSKEEVDIFYKRFSKEAFWPTLHTFWERGQFREDDWQVFLKVNRAFAERTALEAAEGATVWLHDYNLWMVPGYLRELRPDLRIAFFHHTYFPSADVFNVLPWRRQIIGSLLQCDYIGFHIPRQVENFVDAARGVMPLQTVSRQNCAPRFITYGCAVGLERMTTAVDTGNRVVKLGAHPVGLDIERVRSALAAPKIREMMERLRSELAGVKLILSVERLDYTKGILEKLNAYERLLEENPELLGKVTLVTVCVPAAKEMTIYDELQGQIEQAVGRINGRFARIGWTPLQFFFRSLPFEEVSAWYAMADVMWITPLRDGLNLVAKEFVAAQGLLDGRGVLVLSEFAGAAAELKGALLTNPHDPVDMTQTCYVALNMPKAEAQARLRELFDIVNYNDIRRWGDEFLAAVSDPQEQLDRALGLVG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein with magnesium ion binding is needed to enhance glucosylglycerol biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "MIYGIGTDLVDPARIASSLERYGEQFARRVLADSEWPDYLEHIKPALFLAKRFAAKEAFSKATGTGLRAPVMFGNMAVQHDSQGKPYFEFQQELAEWIGQRGITRHHLSISDELTMVSAFVVLQ",
    "response#2": "MIMGIGTDILEISRVIQYIDDNKFLTRILHPDERERFDTFQSEKRKAEYLAGRFCVKEAYAKALGTGIGKLSFQDICCLNDVSGKPYIKDDTNAHVSIAHTDTIATAYVIK",
    "response#3": "MIYGIGTDLVDPARIASSLERYGEQFARRVLADSEWPDYLEHIKPALFLAKRFAAKEAFSKATGTGLRAPVMFGNMAVQHDSQGKPYFEFQQELAEWIGQRGITRHHLSISDELTMVSAFVVLK"
  },
  {
    "reference": "MSGSQFPLHVRDLIRPGDMFAGNLNAQLTGIQHDSRRVEAGNLFVAVRGGKSDGHSFIGQAIGKGAVAIIYEQGRTELDAFLRDYPAIAWIGVNDSREALAGCAARFYGYPSSEIGVIGITGTNGKTTTSYILKSILEALQHKVGLIGTINYMVGDRVYAASHTTPEAADFQKLLRTMADAGCGYAVSEVSSHALAQRRVDGTQFRAAVFTNLTRDHLDFHGTMENYFHAKQRLFAELLIPGGIAAVNVDDAYGRTLVDQLRSEQDHGKDIRILTYAMHNEEADVYANRVDPGFEHTSCTFRIKTSGGERFLDLESPMIGTTNVLNILAAVTVAVGLGVPDSALKEGIKSTAVVRGRFERVAVGQRYLAIIDYAHTEDALERLLQNARALLELSGAGRRPARGKKRDDYLFLPQVNGQGRIITVFGCGGNRDQGKRPRMGEIASRLSYFVILTSDNPRFEDPKAIVREIEAGIRKDNYIVIHDRRAAIAMAVELASPGDIIVIAGKGHEDYQEIKGTRHLFSDREVLEEAIRRSMERHARGARRTKRSGMSRTC",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must bind to Mg(2+) in order to perform its enzymatic function.2. The designed protein should be able to compete with endogenous proteins or inhibitors for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate binding.3. The designed protein should have UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, magnesium ion binding, ATP binding within cytoplasm to regulate regulation of cell shape. The designed protein sequence is ",
    "response#1": "VTGTNGKTTVVSLIKALLDSYQKPCGLLGTIEHILGEGVIKDGFTTPTPALLQKYLATMVRQNREAVAMEVSSIGLALGRVAYTNFDAAVLTNITLDHLDFHGSFETYVEAKAKLFSFIPSSGLAVVNADSPYALPCTENTSAKIVTYGIETRADYQATDIQLSCFETKYTLIHREEKFACSSLFIGKHNVYNMLAAISAALHIGLDPEEVLRGIQVLPGVPRLDPVLIGRCPIYIDYAHTPDALDNVLTGLSELLPKEGRLIVVFGCGGDRDRSKRKLMAQVVERYGFAIVTSDNPRGESPEDIINEICQGFYSKNYCIKIDRKEAITYALSIASDKDIVLVAGKGHEDYQIIGHTKHHFDDREEARKALAERVV",
    "response#2": "ITGTNGKTSTAHMVEAILNHAGRPCANFGTITFRVGGEELPAPHTTPFAEDLVALFQKAQDSGQRHVVMEVSSHALDQERVAGILFDVAVFTNLTRDHLDYHQDMEAYFLAKRKLFERVGQFHPPKENGIAPFTVVARNGEYWERFRQASPVPCLTCGPGGEIVAEKIQMTLSGSRFLLVTPGGTVQISMRMTGGHNIQNALCAAAVALAMDVPFKAILAALEKFKGAPGRMEFCHGLGEQSPRVVIDYAHSPDALSAVLQSIRSMTAGQIWCVFGCGGDRDKGKRSLMGQAASMFADRTVLTNDNPRNEDPSSILDSILAGMNRSPDAVIEDRKSAIKFAIDSAGADDTVLIAGKGNETFQLVGDKTFPFDDVEQASVAVKRLKELVGA",
    "response#3": "MTRLDPVLIGRCPIYIDYAHTPDALDNVLTGLSELLPKEGRLIVVFGCGGDRDRSKRKLMAQVVERYGFAIVTSDNPRGESPEDIINEICQGFYSKNYCIKIDRKEAITYALSIASDKDIVLVAGKQTA"
  },
  {
    "reference": "MAGLSKDQLIESISSMSVIELSELVKALEEKFGVSAAAPAPAVVAGTSPAVATEEKTEFNVILGSVGTSKINVIKVVREVTGLGLKEAKDLVDGAPKTVKENVAKAEAEEIKKKFTEVGATVELK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be involved in translation by localizing to ribosome, ribonucleoprotein complex and exhibiting structural constituent of ribosome.2. The protein must exhibit the following characteristics: Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors; Is thus essential for accurate translation.. The designed protein sequence is ",
    "response#1": "TPPTASLIMKELGIQKGSGNAGSEVVGNISISQVAKIARMKKEDVLSYDLKATMKEVMGTCVPMGVTF",
    "response#2": "GPIGCKVTLRGEAAEGFLETSLGIVEKRLNESQFDIFGNVSFGVEEHTDYPGMRYDPNIGIFGMDITVVVNRPGYRVSKRRIAKRKIPTSHKITKEDTIL",
    "response#3": "TPPTASLIMKELGIQKGSGNAGSEVVGNISISQVAKIARMKKEDVLSYDLKATMKEVMGTCVPMGVTF"
  },
  {
    "reference": "MPAPQPFLPLLFVFVLIHLTSETNLLPDPGSHPGMCPNELSPHLWVDAQSTCERECTGDQDCAASEKCCTNVCGLQSCVAARFPSGGPAVPETAASCEGFQCPQQGSDCDIWDGQPVCRCRDRCEKEPSFTCASDGLTYYNRCYMDAEACLRGLHLHVVPCKHILSWPPSSPGPPETTARPTPGAAPMPPALYNSPSPQAVHVGGTASLHCDVSGRPPPAVTWEKQSHQRENLIMRPDQMYGNVVVTSIGQLVLYNAQLEDAGLYTCTARNAAGLLRADFPLSVLQRATTQDRDPGIPALAECQADTQACVGPPTPHHVLWRFDPQRGSCMTFPALRCDGAARGFETYEACQQACVRGPGDVCALPAVQGPCQGWEPRWAYSPLLQQCHPFVYSGCEGNSNNFETRESCEDACPVPRTPPCRACRLKSKLALSLCRSDFAIVGRLTEVLEEPEAAGGIARVALDDVLKDDKMGLKFLGTKYLEVTLSGMDWACPCPNVTAVDGPLVIMGEVREGVAVLDANSYVRAASEKRVKKIVELLEKKACELLNRFQD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Protease-inhibitor that contains multiple distinct protease inhibitor domains.2. The protein contains novel Kazal-like domains that confer a unique function or activity.3. A protein that contributes to negative regulation of transforming growth factor beta receptor signaling pathway by functioning in extracellular region with receptor antagonist activity. The designed protein sequence is ",
    "response#1": "ITLRGASYVSYRIYDWKDRVHSSTRRISLMFRTNFDDSALFYASGESLKHQYIAASIKNQSVHVEMDFGDNVMSTVLTDDLTRGYWHNLTILHEQRTVSIILDQQQKVLELPATASGNMLFDPEIYFGGGPELHKKKGLASHNNFVGSLKYVYYC",
    "response#2": "ATIITLRGASYVSYRIYDWKDRVHSSTRRISLMFRTNFDDSALFYASGESLKHQYIAASIKNQSVHVEMDFGDNVMSTVLTDDLTRGYWHNLTILHEQRTVSIILDQQQKVLELPATASGNMLFDPEIYFGGGPELHKKKGLASHNNFVGSLKYVYYNDISILYELQRGNPKVHYHGVLEAEFQ",
    "response#3": "ITLRGASYVSYRIYDWKDRVHSSTRRISLMFRTNFDDSALFYASGESLKHQYIAASIKNQSVHVEMDFGDNVMSTVLTDDLTRGYWHNLTILHEQRTVSIILDQQQKVLELPATASGNMLFDPEIYFGGGPELHKKKGLASHNNFVGSLKYVYYC"
  },
  {
    "reference": "MAEEEENQELYIAENEVEEVIKDNDKLMDQGVTEDEAEQEQNDTMNNMVMDMSVQGFFEHKDSVFSVSINPVHSNLCASGGGDDLGYIWDITTGEQICQLTGHKDSIVAIDWSFDGTYIATGGMDSQVRLWKSSTGFEFITAFETVDEIVWLSWHPKGLFLAAGCNDGSIWMWSLPSGKVVQVMYGHTAPVNAGKFIPPGVGKRLATVDDSGTLIVWNPATGAPECRMSSDDHRFDPGNEETAAGWTSFDCNAEGNVLFLGGSSGKVKVVNINSSHILASLEAQTESVEAIALCTALPICACASVDGTVALYDSASLKFRKSLPHEQAVIDCKFLPNTPYLLTACADCVIRKWDVRSGQLLGEYTGHQEPILCMAITPDGKRVVTGSDDTELLVFDCEH",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be optimized for stability and solubility when expressed in nucleus, cytosol, preribosome, large subunit precursor where it will be involved in ribosomal large subunit assembly. The designed protein sequence is ",
    "response#1": "DALELTVRKSRAYGGVVRGLHESAKLIEKRNAQLCVLAEDCNQPDYVKLVKALCADHSIKLLTVPSAKTLGEWAGLCKIDSEGNARKVVGCSCLV",
    "response#2": "LALELTVRKSRAYGGVVRGLHESAKLIEKRNAQLCVLAEDCNQPDYVKLVKALCADHSIKLLTVPSAKTLGEWAGLCKIDSEGNARKVVGCSCLI",
    "response#3": "ALELTVRKSRAYGGVVRGLHESAKLIEKRNAQLCVLAEDCNQPDYVKLVKALCADHSIKLLTVPSAKTLGEWAGLCKIDSEGNARKVVGCSCLI"
  },
  {
    "reference": "MIYETAPAKINLTLDTLFKRDDGYHEIAMIMTTVDLNDRLSFQKRKDKKIVVDIEHNYVPNDHKNLAYRAAQLMMETYDLNEGVTITIDKDIPVSAGLAGGSADAAATMRGINRLFNLDKSLHELSDLGIQIGTDIPFCIYNRTAVCKGRGEKIRFLKKPPSAWVVLAKPNLGISSADVFKALDLDDAHHVDTEMCEKAIVEGDYKQLCESLSNRLEPVSMSMHPEIKKIKNNMLQCGADGALMSGSGPTVYGLAQKESQAKKIYNAVNGCCNEVYLVRLLG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The active site active site of the designed protein must be optimized for substrate binding and specificity.2. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.3. The protein design must be able to perform ATP binding, 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity in order to contribute to terpenoid biosynthetic process. The designed protein sequence is ",
    "response#1": "AMGFDVHRFEAGEELWLGGILVPHDRGLSGHSDADVVLHALTDALLGAIGEGDIGSHFSPSDPQWRGARSSVFLAHARDLIRARGARIDYVDVTIICEAPRLAPYREAMRQEIANVLVLHLHSVSVKATTTERLGFAGRGEGIAAQAIATV",
    "response#2": "MLVGNGFDVHAFDKESEMVLGGVNIDVDFGFKAHSDGDVAIHALIDALLGAAGMGDIGMLFPDTDNAYKNIDSKGLLRETVVKIRQFGYEIVNADVTIMAQVPRLDRYKIKMRKALSAILQIEPSRMNIKATTTEKLGFVGRKEGVGVIATAALKV",
    "response#3": "MLVGNGFDVHAFDKESEMVLGGVNIDVDFGFKAHSDGDVAIHALIDALLGAAGMGDIGMLFPDTDNAYKNIDSKGLLRETVVKIRQFGYEIVNADVTIMAQVPRLDRYKIKMRKALSAILQIEPSRMNIKATTTEKLGFVGRKEGVGVIATAAY"
  },
  {
    "reference": "MNASRPLGPVESIEQLRLWNPLPTPEQMAGWDRWTMDEFGLLEEVLMENASRVALDVLRERFPRDGALGSPDSLAGKRVLLLAGPGNNGGDAIALARHLLDQNAIPLVLTARRPDEYTGTTQKHLRHAQNAGVPFGSLDAVDWLRPMAQLPPEHDPDGFCDIVVDGLLGTGFSGELRDNFQAWVQAVNALRDRVFILAIDVPSGLDGTTGKPSPVAIRAHVTVTMEAAKTGIALPEAAPWVGRLEVRRIGIPRAVRFERPVHRGLLTDAVATLVAPPDPALHKGTAGRVCIIGGSAGLTGAPALAGLGALRGGAGLVTIACPGGLATEVKSGLPECMTLPLGDGVALTPENYEHLSERIPEFDVLVLGPGMGRDPETARLLKRVLSQEDRPPAVVDADGLYHLAQRPSLLGMLTESDVVTPHPGEMARLLAAVDSVDEATAMKRYADNRFAVAEDFAALYPCCLVLKGAGTVVAQRVPEQDEVTSFLCAIACPELAIGGAGDVLAGLLAALRGRDGSADLPLPTACLGVYWHASAGRILSRALPGRGAMAREIAHALPQALKELIHAQSQGHHDHGRGHR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding.2. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. The designed protein sequence is ",
    "response#1": "MRRIDRYAIDHIGMPAMVLMENAGRAVSEEVISFLEGRPGRIAILTGKGNNGGDGIVAGRHLSEAGYQVHLIYAESPDRFQGEAALQSQIAHKWGLPHYIYSEQERINWELYDAVVDALLGTGSKGAPRGVYAKLIEEVNRSGRPVISIDIPSGLDADTGQIHTPCIRADRTVALAFTKRGLEQYPGVRQAGTVIVRSIGIPQGIADGVDVAAAWPMPGMVDDKYTQGVTGIAAGSATYPGAAVLATGAAVLATSGMVRYAGPAADVVRSRWPEVVATGAVTDAGRAQAWVAGPGIGTGHEGRQVLRHVLGLGVPVCADADAITILASNPEVLDARDPGTPLVLTPHDREFARIAGAEPGADRVTAARELASRVDAVVLLKGHSTIVAAPDGRALVNVPEGSWLATAGTGDVLAGLVGALLAAGLDPWLACGAAAYVHSLSARLAAEGAPVSASGVQAAIPAAIRAI",
    "response#2": "DAHKGTFGTSLIVAGSLPYPGAAILAGVSAYRIGTGLVTMAVPKEIYSGLIKTIPEATWIILEDLSGRITENAVQQIKDALGRPAACLIGPGLSQADTTARFLEGVIELENLPSLILDADGLRLLSRLENWPRKLPSGSVLTPHPGEMAVLTGLSVDEIQADRVGIAEHYARDWDQILLLKGAHTVIASPGGGSRILLSADPALARAGSGDVLAGIICGLIAQGMDPSTAAASGAWVHARAGRL",
    "response#3": "LILSGPMGRSGAARLAARAALRVGAGLVTVAAPGSAMLECATQLTAIMLRKCNDADGLAALLEDKRLNALCLGPGLGVGEGTRALVAAALTDQDRAAVLDADALTSFANAPETLFVLLHAKAVLTPHAGEFARLFPDIAEQLNAPATKGPAYSKVDATRAAAVRAGCIVLYKGPDTVIADPTGRCAISAAVYDRAAPWLATAGSGDVLAGLITGLLARGLPPMQAAETAAYLHVEAARAV"
  },
  {
    "reference": "MNRQLHKFGVTTLSDADGIKRIVSLLKKYGQPNDLIVVSAMGNTTSLLMNWVNLSKENPVEANRTLQMIRHQYLIHASALLKKPESVTLPFLDDILTLSARLDQTLTEATYAEIVGHGEIWAARLLTAYLNQQGMPAEFIDARRFLTAERHVLPTLLTVPSQQKLQPILTEHADKYLVISGYISQNDAGETVLLGRHGADYSATKTAALADIEQVTIWGEIAGVYSADPNLVKNASLLSLLRLDEATELARLSAPILHPNSLQPVSEAMLKLRLRSTREPEKGFTQVEHLLASSAQGKIVTHHNQIGLIELVIPLSQDIKQWENQFYTWLQQLQLNPMALSLDENERTLKLVYPNELIDDIFIKFKGLPLPTESTSIRYDLAMLGIIGANICQNSLQMMRFFQQIKNQPIELIWQSPQNISVVAILRQPVTRELLKNVHDDLFRAHKTIGLVVLGKGEVAKTWLQLYQQEREKMAARSHFDFTLMGIVASQKAWLDYQGLSHLTVNHLDQLFEQHAKELQPAELLNWLSLHPFDELVLLDITASDFIAEHYINYAKHGFHVVSANKRAAAFPLGRYHQIEEAFSLAGSYWLYNATVGSGLPITTIVHDLVESGDKILSVEGTFSNSLAWLFYQYDGSTPFSELVEQAWLQGLMERDPREDLFGYDALRKLVITARSAGYALDNHTVKCHGLIPKQLADLSITDFFEQLSQVDDDIYQRYQAAQEDGLVLRYIARFHAPNQATIQLEAIDPEDNIAKTLPGENLFMIKS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. We need a protein that can perform L-homoserine + NADP(+) = H(+) + L-aspartate 4-semialdehyde +   NADPH with high efficiency.2. The designed protein must have at least one Homoserine dehydrogenase catalytic, Aspartate/homoserine dehydrogenase NAD-binding domain.3. We require a protein that can specifically bind to the metabolites produced in the Amino-acid biosynthesis, L-threonine from L-aspartate: step 3/5, L-threonine biosynthesis pathway.4. The protein design must be able to perform NADP binding in order to contribute to phosphorylation, threonine biosynthetic process. The designed protein sequence is ",
    "response#1": "MRVLKFGGTSLSNSERFMNVADIVVNNQQQSQVALVLSAPAKVTNHLVAVVEQTIRGLDPAPVLQEIEQIFHDIIEGIKATYAELDEAGLKGRIERELGQLSRLLQGVSLLQQCPDNTQAHILSRGEALSIATMAELLKARGEEVEVITPQTMLLGEGSFLEATINIKVSRQRFADQGLRDDCIYLMPGFTAGNDKGETVILGRNGSDYSAAVLAACLNAECCEIWTDVDGVYNCDPRLVPDAKLLKQLSYKEAMELSYFGAKVLHPKTISPIAQFSIPCLIKNTANPQGEGSLIAVGDGMAGTPGVSARLFGALGRAQVNILAIAQGSSERNISVAIDSADATKALRAAHAGFWLSPQTFAIGVIGPGASVSLVGDGMRQHRGVAAKFFASLAQARVNVVAIAQDSSERSISAVIEQSACKNAVKVCHENAASCHVHKFGGSSLADAAKMRAIGALLGETGTRLVVVSAMQGTTNALVAMAEAAREGRDWALDWEALRQRHLDAAETLGDADVRETIAAGFDDLRADLLELAADPADAQTLAARVHGAGEIWSSHLVRAALGDGWQRLDAREALVVHPGEMGVGVDWDESRRRFARWRAEHAGRDTVVTGFVARDAQGRDATLGRNGSDFSAAIFANLCDADALTIWTDVDGVLSADPRLVPDAVLLPSLSYAEACELAYFGAKVLHPQTLAPVQSRGIPLRIRNTLRPQAPGTEIVAKHSADGTPVKGLSLVRDLAVLELVGNGMVGVPGAAERLFAALHGAGVSVTMISQGSSEHSICCIVRADQAERGRAAIAQAFAGDMADQRTQAVRVTQDISVLAAVGDGMVGTPGVAARLLGGLAQARVNVRAIAQGASERNVSVAVASDDATRALRAAHSAFWLSPQHVSMGVIGPGQVGRALLSQLAATLPKLRARSQLDLRIRGLCDSRRMALSETGFDPDSALAALQEGAANPIDLDAFAAHIRGEHLPHALIVDCSASDAVAARYPQWLAAGIHVVTPNKHAGSGEWARYAAIGEAQRRGGARFLYEATVGAGLPVIQTLRGLLDTGDELHGIDGMLSGTLAWLFNRFDGSQPFSALLREAMALGYTEPDPRDDLSGLDVARKLVILAREAGRELSVEDVDVESLVPASLQKIGREDFLARLEEIDAPMQARLDAAAARGLGLRHLARLGADGSAQVGVVALPAD",
    "response#2": "MKVLKFGGTSLKNAEKFLLVTNIINIYLKKSKIAVVVSAPATITNSLEIMIEKSIKKKNYEQDFIYIQKFFINLINNLKKQINDLDIIFTKKKINKIFDKIKQLLYGINLINNCPDIIRAKILSQGEKFSIILLESLLKIEKYKIYIISPKKYLLAYGNYLESTVDISLTSKNIKNINISNIDIILMAGFSAVNKKKETVLLGRNGSDYSAAILSVCLKAKYCEIWTDVNGIHTADPNLISNTKLLKFISYQEAITLAYFGAKVIHYKTLFPMLKNNIPCIIKNIINIKSNGTLISNNNIKNSLHKIKGITELKNISMLNINGEKNKQIKNIISRILFFIFKLKISIYFITQSSEMCNISIFILDKNLKYVKSLLEEEFYLEFQHKLIKPISIIQQLSIISLVSNQINNSKNIISDFLNIFKITKIKIFAISQNIFQNYISVIIKENYAKNIIEITHKTLFHKEKILEIFVIGTGGIGNTLLKQIYQKKNYFKKKNIILKICGIFNTKYSLININGLNIKNWKQNIEYNPNINIIKILLNNFKKYKFINPVIIDCTSSQEIANQYVNFFNCGLNIVAANKKANSSKMDYYNQIHFLANQLNLKFFYETNVGAGLPVISTLQNLLKTGDKINYLIGILSGSLSFIFGKLEEGITFSKTIKIAQKMGFTEPDPRVDLSGIDVARKLLILAREIGYNLELNDITIESIIPNTYNKKISIEEFFVSLLDLDKIYKKKIQEAKKDNKVLRYIGSIDKQGFCKVEIVKIDKNH",
    "response#3": "MKVLKFGGTSLKNAEKFLLVTNIINIYLKKSKIAVVVSAPATITNSLEIMIEKSIKKKNYEQDFIYIQKFFINLINNLKKQINDLDIIFTKKKINKIFDKIKQLLYGINLINNCPDIIRAKILSQGEKFSIILLESLLKIEKYKIYIISPKKYLLAYGNYLESTVDISLTSKNIKNINISNIDIILMAGFSAVNKKKETVLLGRNGSDYSAAILSVCLKAKYCEIWTDVNGIHTADPNLISNTKLLKFISYQEAITLAYFGAKVIHYKTLFPMLKNNIPCIIKNIINIKSNGTLISNNNIKNSLHKIKGITELKNISMLNINGEKNKQIKNIISRILFFIFKLKISIYFITQSSEMCNISIFILDKNLKYVKSLLEEEFYLEFQHKLIKPISIIQQLSIISLVSNQINNSKNIISDFLNIFKITKIKIFAISQNIFQNYISVIIKENYAKNIIEITHKTLFHKEKILEIFVIGTGGIGNTLLKQIYQKKNYFKKKNIILKICGIFNTKYSLININGLNIKNWKQNIEYNPNINIIKILLNNFKKYKFINPVIIDCTSSQEIANQYVNFFNCGLNIVAANKKANSSKMDYYNQIHFLANQLNLKFFYETNVGAGLPVISTLQNLLKTGDKINYLIGILSGSLSFIFGKLEEGITFSKTIKIAQKMGFTEPDPRVDLSGIDVARKLLILAREIGYNLELNDITIESIIPNTYNKKISIEEFFVSLLDLDKIYKKKIQEAKKDNKVLRYIGSIDKQGFCKVEIVKIDKNH"
  },
  {
    "reference": "MIRAIFKSLCPNCDIEDIDSERLEKGFACKKCMPDDKDIVRDGYLKHIFSKERLLEEINRIFLEYVKAPMWSLQRLWARRFFDQESFAMLAPTGSGKTTAQIILGVYAAYKMNKRVLYLLPTSLLAHQVTEKINELINTLGTKDLIVFSYHSYLKDQERREQLEKINNAKILVTTTMSLIKRPEIHNQRVDIVFVDDVDSFLRKSKSIDYVLKMIGVDEKLRKEVEELIDEERGLRKLIKSEPDKYEEELKRIISRKTEIKRREFGQIIVSGATQTTKKTRRILMLETLLGFSVGRRIEVGRKVVDAFIEPEDKRSLEQIIIDVIRRLGTGGLVYVPLDKGSDYAEALEKILVGNGIKAKSFTKSSRKIFEAFVSEEYDVLIGIASSRSPLVRGIDLPERVRYAVFAGVPKFKIKIDINEFHPTKWLILLNSIRDIIPREYQDEIDFIVGSLSNLRYLRKEDLEKIREAVKNGQTLEGFLEYARRNAERTLRFFERILSDKNILEAMRKSPYISLSEKRGEYVFVVPDVAAYIQASGRTSRLFAGGVTQGLSILIIDDVKAFNALMREMRWYIDEVSWIRFKDLDLDDLIRKIDLDRERVRMIRSGKAIGEFKDLIKTRLFIVESPNKARTIGRIFGRPVQRAFMDLQTYETVIEDSLLIITASGGHVLDLSMREGLFGVLIEKDVSNENIKSFVPVYTVLKKCMNCGRTVPEEAVKCPYCGSTVFRSVKNSINALRLLASQVDEVIIGTDPDSEGEKIAWDLYLLLK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must be optimized for hydrolase activity. The designed protein sequence is ",
    "response#1": "EFGENILKFKKFVNGNIRGISTPDGKLLDGMNEHGLVIFVFYFKNYAKYGCPSQTKLNIKPTEVALFLLQKAKNVKDVKAIAKTLNVIHESYPPFTETPPMHWLVTDASGKSIVLEPLGNGELTVFDNPMGIFTNAPTFPEHMESAKKALEHLSPISDPNAASQGTGALGLPGDFSSASRFIRLAFFSQTIEIPRTSAGAVNTLFHVLNNFDIPKGVVASINMNTGKHVYEKTIYTVIYNIKSKEIVFKHYNDQNIQRC",
    "response#2": "MGFLHGGLSVALAETIGSMAGFCCITENQFVLGLEINANHLRPVKQGIVTARATPIHLGTRTQVWQIEIKDQQDQLCD",
    "response#3": "MGFLHGGLSVALAETIGSMAGFCCITENQFVLGLEINANHLRPVKQGIVTARATPIHLGTRTQVWQIEIKDQQDQLCS"
  },
  {
    "reference": "MFESEVRHYLHRDLRGSTTAVRVYNLVQKQRLASSLDEPQVELDEFSIKRSSPPCRYYAPPPKYSCKKRSRSSDEDEDSDPNQPGPSNRRSPKTGNKRVRGNGDNTELYIPNRYELIVSGKSVGGNTSAAWNGPGSTLEQNMNALEECFELGVDEYDFDEHDGDEGCELVQDMLDRERNLMNKRSTAQRRESRNIEKMKKSQENLDKEKSEEKISESKKNQDDSIESPNLPGTPANLPVDEPLPPVGRLYIVEHVLPEEYRRDPLTEPPSMEDCIRAHSDPNLPPHPRADQYPASFTRPMVEYCEDIYWTHRTGQLPPGLQVPRRPYDYYHITERTPPPEDLNWVPPAESPPIPIPQQAFDLLEERRRNHREQQDEAREGDLSDPEV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that contributes to hermaphrodite somatic sex determination, oocyte morphogenesis, female gonad development, oocyte fate determination by functioning in perinuclear region of cytoplasm, plasma membrane, nucleus, cytoplasm with transmembrane signaling receptor activity.2. Prioritize Basic and acidic residues, Pro residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "LLPISMCFNIKNTDMAEPSRVSDRNIHMIIDESGIILKMTPSVYEPADLLSGEVPLNCASLRSKEFNIKKLYTAYQKVLN",
    "response#2": "LLPISMCFNIKNTDMAEPSRVSDRNIHMIIDESGIILKMTPSVYEPADLLSGEVPLNCASLRSKEFNIKKLYTAYQKVLC",
    "response#3": "LLPISMCFNIKNTDMAEPSRVSDRNIHMIIDESGIILKMTPSVYEPADLLSGEVPLNCASLRSKEFNIKKLYTAYQKVLS"
  },
  {
    "reference": "MNSTPPTSPVTRVSDGSFPSISNNSKGFAYRQPQKHKSNFAYSHLVSPVEEPTAKFSEAFQTDYSSKAPVATSEAHLKNDLDVLFTTPRFYSPENLALMFRLSNTVSSLEFLDEFLMGILLAPEMDFLSNPSYSLPSNKLVGQGSYSYVYPISSSASSRCNNDSGVVLKFAKSQHKSKVILQEALTLAYLQYMSPSTNESHIIPFYGLTYITKSHFRRLRSNECVPGLILPKCEMSLYHFNTAVSHKLSLITKRKIWWRLMKQMIDALKSLKTNGIIHGDIKTANILITEMHVLNGGHCKDFDFYLADFTSAFHINQTPTDLNTTVEYCAPELIDSSSDHVPTFESDLYAVGLCLLSFISQHEPYNELQALVSHGSSPGIGSSSIQQSQWLINALLKKDPINLNMLRNDLFQDWKSELALLSRILVDRLPLENLITILDSNYI",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Probable serine/threonine protein kinase which may function redundantly with MPK1-independent branch of the PCK1 pathway that is presumed to be required for the tolerance to high temperatures and staurosporine..2. The protein design must be able to perform protein kinase activity in order to contribute to negative regulation of UDP-N-acetylglucosamine biosynthetic process.3. The protein should have a low Km value for ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-   threonyl-[protein].4. The presence of Protein kinase domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "YQIIKTLGEGSFGKVKLAQHVGTGQKVALKIINRKTLAKSDMQGRVEREISYLRLLRHPHIIKLYDVIKSKDEIIMVIEFAGKELFDYIVQRGKMPEDEARRFFQQIIAAVEYCHRHKIVHRDLKPENLLLDDQLNVKIADFGLSNIMTDGNFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSSGVILYVMLCGRLPFDDEFIPALFKKISNGVYTLPNYLSPGAKHLLTRMLVVNPLNRITIHEIMEDEWF",
    "response#2": "FQMIEKAGQGASGSVYLAQRLKIPPYDENSGVSQHELNDNIGDKVAIKQMILSKQPRKELIVNEILVMKDSQHKNIVNFLEAYLKTEDDLWVVMEYMEGGSLTDVIENSIGSDASESPMTEPQIAYIVRETCQGLKFLHDKHIIHRDIKSDNVLLDTHGRVKITDFGFCAKLTDKRSKRATMVGTPYWMAPEVVKQREYDEKVDVWSLGIMTIEMLEGEPPYLNEEPLKALYLIATNGTPKLKHPELLSLEIKRFLSVCLCVDVRYRASTPHRFKNYNYMSPTFCDHCGSLLWGLVKQGLKCEDCQMNVHHKCQTKVANLCGQVTRSRLPGLLPPLDDVKNFVGIQNLVAHEPMLQPQTPNGSINPAECWPAVGGGVPSAYDLGTVIGFYGLTIDEPPTGTAPNQVGQLIVAFRDAATAQRHLADLASIWRRCGGRTVTLFRSEWRRPVELSTSVPEVVDGITTMVLTAQGPVLRVREDHAIAAKNNVLVDVDIMTPDTSRGQQAVIGITNYILAEGCTYRVIKTLGSGGFGETFLAEDSQMPSNRRCVVKQLRPIHNNPQIYQLVQERFQREAAILEDLGSYSGQIPTLYAYFQSNTQFYVVQEWVEGDTLTAKLKQQGVLSESAVRDILINLLPVLEYVHSKRIIHRDIKPDNIILRHRDGKPVLIDFGAVRESMGTVINSQGNPTSSIVIGTPGYMPSEQAAGRPVYSSDLYSLGLTAIYLLTGKQPQELETEPHSGEIIWHRYALNISPTLAAVIDRAIAYHPRERFTTAREMLEAL",
    "response#3": "FQMIEKAGQGASGSVYLAQRLKIPPYDENSGVSQHELNDNIGDKVAIKQMILSKQPRKELIVNEILVMKDSQHKNIVNFLEAYLKTEDDLWVVMEYMEGGSLTDVIENSIGSDASESPMTEPQIAYIVRETCQGLKFLHDKHIIHRDIKSDNVLLDTHGRVKITDFGFCAKLTDKRSKRATMVGTPYWMAPEVVKQREYDEKVDVWSLGIMTIEMLEGEPPYLNEEPLKALYLIATNGTPKLKHPELLSLEIKRFLSVCLCVDVRYRASTPHRFKNYNYMSPTFCDHCGSLLWGLVKQGLKCEDCQMNVHHKCQTKVANLCGTEMVLIEGGEFSRGSVEGQRDELPVHKVILHSFFLDVHPVTNEQFIRYLECCGSEQDKYYNELIRLRDSRIQRRSGRLVIEPGYAKHPVVGVTWYGASGYAEWIGKRLPTEAEWEIAASGGVAALRYPCGEEIEKSRANFFTADTTTVMSYPPNPYGLYDMAGNVYEWCQDWYGYDFYEISAQEPESPQGPAQGVYRVLRGGCWKSLKDDLRCAHRHRNNPGAVNSTYGFRCAV"
  },
  {
    "reference": "MKSLVFATNNAHKLEEVKAILEGKYRLVSLSEIGCFDDIPETSPTIEGNALMKARFIVNNYNQDCFADDTGLEVDALDGRPGVYSARYAGEGCSYSDNVEKLLLELKGKSNRKARFKTVIALAMNGTETLFEGIINGIIIHEKRGISGFGYDPVFVPDGYDQTFAEMSLELKNTISHRALAVKKLAESLM",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be designed to have high stability and activity towards dITP + H2O = dIMP + diphosphate + H(+).2. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..3. The protein should be able to bind Mg(2+), substrate ligand in a variety of conditions4. The protein design must be able to perform XTP diphosphatase activity, dITP diphosphatase activity in order to contribute to nucleotide metabolic process, purine nucleoside triphosphate catabolic process. The designed protein sequence is ",
    "response#1": "MILATHNPGKVREFADLLAGLGVRAVSAGELGLPEPVEDGLSFAENALIKARAAARATGLPALADDSGLCVDALGGAPGVLSARWAGRHGDDAANNELLLAQLGDVRDEHRAAAFVCTIALALPDGTEHVVEGRWPGRIARTARGEGGFGYDPVFLPDGETRTTAELAPEEKNRISHRGRAFRALAQLVR",
    "response#2": "MPTITFNRED",
    "response#3": "MIQMLLATRNPGKLREFRD"
  },
  {
    "reference": "MRLPEVLRRSGVETPVPAGADHLSITGITADSRAIRPGMIFAALHGTHMDGRSYIPQAVASGAVAVLLDMSPCHVPAGIVAIRVPDARRMLALVARVLTPHVPECIAAVTGTNGKTSTVEFLRQIWALRGYAAGTIGTLGAVAPCALPDCGPVLTTPDSVGLMRLLGAMAENGVGHVAIEASSHGLEQRRLDGLAPYAAGFSNLTRDHLDYHGTTEHYRAAKLRLFDTVLPEGGIAAINADMDVATCTAITDIARRRDLVLRSTGEQGATLRLLGACPTPTGQILRIATAAMVHEVTVPLVGRFQVDNMLLAAAMADRDVDGPDRAVPLLAQLRGVRGRMEQVAVLSSGAAAYVDYAHTPDALERLLLSLRPHTHGRLVVVMGAGGDRDRGKRPVMGETATRLADRVIVTDDNPRTEQPAAIRAAIMAAAPGATEIGDRRQAIAAGLDMLDAGDVLVVAGKGHEQGQVVGHDVLPFDDATVIRGLTGNE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that contributes to cell division by functioning in cytoplasm with UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity.2. The designed protein should contain one or more Mur ligase C-terminal, Mur ligase central domains that are essential for its function.3. The protein design should consider the endogenous proteins and enzymes present in the peptidoglycan biosynthesis pathway to avoid interference with native metabolic processes.4. The protein design should include a Meso-diaminopimelate recognition motif motif to ensure proper function.5. The protein should be able to bind meso-2,6-diaminoheptanedioate, ATP ligand in a variety of conditions6. The desired protein should be able to perform ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate The designed protein sequence is ",
    "response#1": "MNEVQGLRADSRALQAGELFVARQGARVDGLMLAPDAVARGAVAVLAGHGDAEALRAALGVPVIVLSDLAHALGVLADRFYNQPASQLTLIGITGTNGKTSVSQFIAQSLESLGYACGVIGTLGAGRIDDLNDTGMTTPDPVTLQAILADFCQQSIEYVVIEASSHALEQGRLNSVTLDMAVLTNLSRDHLDYHVDMAGYAAAKKQLFDFASVKTAVINQADDFGKILIPELEGRDDITLLTYSSHSATLMAAIQAKEIQTTIDGLHFNISSEFGSASIKSPLLGRFNVDNLLATLATLLALDYPLQQLIAVMPQLQGAPGRMERFCVQDRPLCVVDYAHTPDALAQALSTLRAHLKPKSGAKLWLVFGCGGNRDVSKRPRMGLVAAELADRVIVTDDNPRDEDGEVIAQGILAGMAQQDHVLIERDRRRAIAFALTEAAPEDIILVAGKGDEDYQIIGTQRLHYSEREVVRAWLARNAA",
    "response#2": "MSLSEITGLSSDSRKVEPGMLFAALAGTKADGAGYIGDAAARGAVAAVSGHIAEAGIPVLAVSNPRRFLSLAAARFYGHPSRALWMTGITGTNGKTTTTYLVLALTEANDERAAMLTTVEYRIGERSEPAIRTTPEASDTQRFLREALNENCLTAVMETSSQAIDLHRCDWLRFRVAVFTNLTRDHLDYHLTMENYFDAKKKLFDGRIGEKPAASVINVDDEWGAKLANELKANNQRVVTFAQNKTADLTAENIEVSLLSGTSFDLKTPQGSRKITSPLVGKPHVYNMLAAMATARGLSLSVEQISAGLASFPGVPGRLELVGQTKDGALAYVDYAHKPDALENVLSSVRPFTTGRIVVVFGCGGDRDKGKRPIMGEIATRLADIVIVTDDNPRSEVPEQIRSEIMAAAPGAIEIADRAQAIRHAVSLLKSGDTLIVAGKGHEDYQILGTEKVHFDDREVARDALAARAK",
    "response#3": "MKLRDLLQGIDYQIVVGDTENILQTDSRQVAPGDAFIAWPGAATDGRLHVADALARGASAVLVERASKGIPDWIVPDSRHALAGLAASWYDHPAQKLQLIGVTGTNGKTTVAFMVKALMDAAGIPTGLLGTIRYEIGDRVIPAARTTPEALEIQQMLSQMVRAGCQACVMEVSSHALDQKRTLGVEFDVAIFTNLTQDHLDYHGTMDGYFAAKMKLFTALQHGVKRGAAVINIDDAYGERLRLQTEVAVKLTYGLGEAAQLRATNLRLDRNQTSFQVAIAGQSVSCQLPLIGRYNIYNALAALGLVLATGLPAEQALQALAALQCPPGRVERFKATDDQPLIVVDYAHTPDALDQVLQALRPHVAGKLWCVFGCGGDRDRGKRPQMGAIAERLADRVILTDDNPRHEAGDAIVREIAGGMRSSARIIRDRREAIATALSEADAADVVLVAGKGHETYQEIQGERYHFDDREQACAALQQRHS"
  },
  {
    "reference": "MTLTPVPHLAVIVVAAGSGTRLQGGAPKAFVGIDDRSILHHALRGVFAAMSAQVVVVVPRGYEGEALTDARNAAGDRADLFTVVVGGATRQESVAAGLAALWADVEIVLVHDAARALTPPAVFDRVVHAVDAGAAAVIPTLPVVDTIKRVDESGRVVGPVDRSELAAAQTPQGFLRSVLEAALAAVDADHTDDAAAVAAAGHAVVTVEGDAAAFKITTGPDLERARALLATEPAASVARPPVHSAPRVGIGTDVHGFGGEGTLWLAGLEWPGEVALSGHSDGDAVAHSIVDALLAAAGLGDIGTHFGTDRPEFAGAHAAAFLSHTVQLLSDAGWRVGNVAVQVQARRPRFADRRIEAEQALSAALGGAPVSVSATTTDGLGFTGRGEGVAAFATALVVPV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The a divalent metal cation binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must bind to a divalent metal cation in order to perform its enzymatic function.3. For general function, the protein need meet that Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D- erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C- methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).4. The protein contains novel 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MRVGNGFDVHKFGPGSFVKLCGVTIENSSALIGHSDADVGMHAVTDAIYGALARGDIGQHFPSSDPKWKGADSQIFLKHAIKLAFDIGYQIENIDCTLICEFPKIGPHAGKMRIKMAEIMGLKVDQVSIKATTSEKLGFTGRGEGIASIATVTLMWR",
    "response#2": "MRVGNGFDVHKFGPGSFVKLCGVTIENSSALIGHSDADVGMHAVTDAIYGALARGDIGQHFPSSDPKWKGADSQIFLKHAIKLAFDIGYQIENIDCTLICEFPKIGPHAGKMRIKMAEIMGLKVDQVSIKATTSEKLGFTGRGEGIASIATVTLMVRVGTGYDVHQLEEGEELWLCGVRVEHTHGLSGHSDADVAIHALVDAMLGAIGAGDIGQHFPPSDARWKGAASDRFLDHASKLVAEAGYALGNADVTIICEAPKIGPHREAMRARLAAILGVDIALVSVKATTTEKLGFTGRREGIAAQAAVSLLY",
    "response#3": "MRVGNGFDVHKFGPGSFVKLCGVTIENSSALIGHSDADVGMHAVTDAIYGALARGDIGQHFPSSDPKWKGADSQIFLKHAIKLAFDIGYQIENIDCTLICEFPKIGPHAGKMRIKMAEIMGLKVDQVSIKATTSEKLGFTGRGEGIASIATVTLMLVGG"
  },
  {
    "reference": "MPLLLSGRGFRRELESAGCMAVHAPLEGGAETRLLRRLRAAGYRTHLTSARGLGDPEVFLFQKHGVRPPHLGHQSVGRGAAVGEVHDVMPLLGEVFLGDKPVVLWLLEGQVLSRSELLSLCDLCRREPRLKIVVEMGGARSLRWQPMTQLLAA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. We are looking for a protein with broad substrate specificity for a plastoquinone + (n+1) H(+)(in) + NADPH = a plastoquinol + n   H(+)(out) + NADP(+).2. A protein that localizes to plasma membrane-derived thylakoid membrane and can perform oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor, quinone binding with high specificity and efficiency.3. For general function, the protein need meet that The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone; Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient; Cyanobacterial NDH-1 also plays a role in inorganic carbon-concentration.; NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain The designed protein sequence is ",
    "response#1": "DVGAQTPFFYIFREREMIYDLWEAASGMRLINNNYFRVGGVAVDLPYGWNDKCEDFCDYFLPKVDEYEKLITNNPIFRRRVEGVGTVTREEAINWGLSGPMLRGSGVKWDLRKVDHYECYDELDWEVQYETAGDCFARYLVRIREMRESVKIIRQALKAMPGGPYENLEAKRLQEGKKSEWNDFQYQYIAKKVAPTFKIPAGEHYVRLESGKGELGIFIQGNDDVFPWRWKIRSADFNNLQILPHILKGVKVADIMAILGSIDIIMGSVDV",
    "response#2": "DVGAQTPFFYIFREREMIYDLWEAASGMRLINNNYFRVGGVAVDLPYGWNDKCEDFCDYFLPKVDEYEKLITNNPIFRRRVEGVGTVTREEAINWGLSGPMLRGSGVKWDLRKVDHYECYDELDWEVQYETAGDCFARYLVRIREMRESVKIIRQALKAMPGGPYENLEAKRLQEGKKSEWNDFQYQYIAKKVAPTFKIPAGEHYVRLESGKGELGIFIQGNDDVFPWRWKIRSADFNNLQILPHILKGVKVADIMAILGSIDIIMGSVDGERECYDMFGIVYEGHPNLKRIG",
    "response#3": "DIGAQTPFFYIFRERETIYDLFEAATGMRMMHNYFRIGGVAVDSPYGWVDKCSDFCNYSLPKVNEYERLITRNPIFLKRVEGVGTIGGEEAINWGLSGPMLRASGVQWDLRKVDHYECYDESDWQIQRQEEGDSLARYSVRIGETKESVKIVQQALKVIPGGPYENLEARRLNRGKNSEWNDFEYQFISRKPSPTFKLPKQEHYVRVEAPKGELGIFLMGDDSAFPWRWKIRPPGFINLQILPQLVEGVKLADIMTILGSIDITMGEVDGMXPXRXXX"
  },
  {
    "reference": "MSFATTNKTIVPCATTKQIVRPRFLSNGTISKSRAMMVWEPFNNKFFETFSYLPPLTDDQITKQVDYILRNNWTPCLEFAGSDQAYVTHDNTVRMGDCASTYQDNRYWTMWKLPMFGCIDGSQVLTEISACTKAFPDAYIRLVCFDANRQVQISGFLVHRPESATDYRLPADRQV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Although the small subunit is not catalytic it is essential for maximal activity.; Both reactions occur simultaneously and in competition at the same active site.2. The ideal protein would be one that plays a critical role in photorespiration, reductive pentose-phosphate cycle through ribulose-bisphosphate carboxylase activity within chloroplast. The designed protein sequence is ",
    "response#1": "ATLETFGFLPPMTQDEIYDQIAYIIAQGWSPLVEHVHPSNSMATYWSYWKLPFFGEKDLNVVVSELEACHRAYPDHHVRIVGYDAYTQSQGACFVVFEGE",
    "response#2": "TLETFGFLPPMTQDEIYDQIAYIIAQGWSPLVEHVHPSNSMATYWSYWKLPFFGEKDLNVVVSELEACHRAYPDHHVRIVGYDAYTQSQGACFVVFEV",
    "response#3": "KFETLSYLPPLSTEALLKQVDYLIRSKWVPCLEFSKVGFVFREHNSSPGYYDGRYWTMWKLPMFGCTDATQVLNEVEEVKKEYPDAYVRVIGFDNMRQVQCVSFIAFRS"
  },
  {
    "reference": "MAVTISDVCAVVPAAGFGRRMQTECPKQYLSIGDKTILEHTVAALLANPRVTRVVIAISPGDARFAALPLANHPQITVVDGGTERADSVLAGIRAAGNVAWVLVHDAARPCLHPDDLARLLAISQTSTVGGILAAPVRDTMKRAEPGLNHIAHTVERVDLWHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPELVEGRADNIKVTRPEDLQLAEFYLTRMTYQENA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein with 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity is needed to enhance isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway, terpenoid biosynthetic process in a specific context.2. We are looking for a protein with broad substrate specificity for 2-C-methyl-D-erythritol 4-phosphate + CTP + H(+) = 4-CDP-2-C-   methyl-D-erythritol + diphosphate.3. The protein should be optimized for activity in isopentenyl diphosphate biosynthesis via DXP pathway, isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 2/6, Isoprenoid biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The protein must exhibit the following characteristics: Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D- erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).. The designed protein sequence is ",
    "response#1": "MERELAPTISTRTGLGFDVHRLVAGAPLWLGGIEIAHSHGLSGHSDADVAIHALVDALLGALAEGDIGSHFPPSDPQWRGARSERFLAFAAGRVRARGGRIEHADVTIICEAPKIGPYREAMRARLAAIMGIGIERVSVKATTTERLGLTGRGEGIAAQAVATGVPLGRHDFTDDASIAEWAGLPVTLAEGSVDNIKLTVKRDMAMADEKLSRFVVPDVRTGNGYDVHQLEPGDGVTLCGVFIPHHLKLSGHSDADVALHALTDALLATCGAGDIGDHFPPTDMRWRGAASTIFLEHAAKLVRDRGGVITNADVSLIAEAPKIAPHRDQMRKNLADTLDITIDRCSVKATTNEKMGFVGRNEGIAAIATATVLGRHDFTDDASIAEWAGLPVTLAEGSVDNIKLTVKRDMAMADEKLSRFVVPDVRTGNGYDVHQLEPGDGVTLCGVFIPHHLKLSGHSDADVALHALTDALLATCGAGDIGDHFPPTDMRWRGAASTIFLEHAAKLVRDRGGVITNADVSLIAEAPKIAPHRDQMRKNLADTLDITIDRCSVKATTNEKMGFVGRNEGIAAIATATVRVGVGIDVHAFDDSSPLWLAGLHWPGERGLSGHSDGDPVAHAVCDALLSAAGLGDIGSRFGTADPRFQDAHGEVFLRATRDLVEAAGFTIGNVSVQLIGNRPRFSPRREEAESLLSALLAAPVSVAATTSDALGFTGRGEGVTAVATALLV",
    "response#2": "MERELAPTISTRTGLGFDVHRLVAGAPLWLGGIEIAHSHGLSGHSDADVAIHALVDALLGALAEGDIGSHFPPSDPQWRGARSERFLAFAAGRVRARGGRIEHADVTIICEAPKIGPYREAMRARLAAIMGIGIERVSVKATTTERLGLTGRGEGIAAQAVATVKIVDGEIGRHDFTDDASIAEWAGLPVTLAEGSVDNIKLTVKRDMAMADEKLSRFVVPDVRTGNGYDVHQLEPGDGVTLCGVFIPHHLKLSGHSDADVALHALTDALLATCGAGDIGDHFPPTDMRWRGAASTIFLEHAAKLVRDRGGVITNADVSLIAEAPKIAPHRDQMRKNLADTLDITIDRCSVKATTNEKMGFVGRNEGIAAIATATVVAVV",
    "response#3": "MERELAPTISTRTGLGFDVHRLVAGAPLWLGGIEIAHSHGLSGHSDADVAIHALVDALLGALAEGDIGSHFPPSDPQWRGARSERFLAFAAGRVRARGGRIEHADVTIICEAPKIGPYREAMRARLAAIMGIGIERVSVKATTTERLGLTGRGEGIAAQAVATSVEVVRSDAERGAAVAATETRTGIGFDVHRFGPGNHVMLGGIAIPHIAGLIGHSDADVALHAATDAILGAMAAGDIGSHFPPSDPRWRGAPSAHFLRHAAGLVARAGGEILHLDITIICERPKIGPYRPAMTVCIADILGLDPRRVSVKATTTERLGFTGRSEGIAAQAAATAVRVTAR"
  },
  {
    "reference": "MRIEDVYIWDINARWLGITPHQLMENAGAGVARVIEERFGRGLRIAVFSATGNNGGDGFVAARHLSFENDVTLFLVGDEAKIRSEEAKHNWEILKRLDFVKIRVLKDSAHIKELDLSGFDVIVDALLGAGTKGEPREPIRSAIEKINEYAGRAKIVSIDLPSGYPSTVRVKADFAVTFQWDKDEYEGFERVVVKIGYPKELYNIVGPGDAKFALRKKGEHKGQNGRLLIIGGSEDYFGAPYLAAKAASYLVDLVFLTMPEYSARRIGDPNLILRPVEGSNFALKHLENVLTIAEKVDAVVIGPGIGVDENTKAFVREFIRRCGRPMVIDADALKAIAGDLDALRGKRFVLTPHAGEFRMLFGELPPAGLKERAATVMKKAKEIGGVILLKGSYDVISDGKTWKYNKTGNRGMTTGGTGDVLAGIVGALLALGNEPLRAASVGAFLNGLAGDMVKEEKGENFTALDVAEKIPNATRWALEF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Mg(2+) is required for the proper folding and stability of the protein.2. For general function, the protein need meet that Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP3. The protein should have a fast turnover rate for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "GVVAGGEDYTGAPILACTAAVSAGVGMVRYVGTPTPEQLVRTLVPEVVLGPGRVQAWVVGPGLDVTSRKAGAKAQVDVAKEALSSDLPVLVDAGGLDLVHGRRSAPTLLTPHAGECARLLTRLARRKRAITPDEVKAAPLHHARRLAALTGASVLLKGSTTLVVSPDPDAPVHSQRDAPPWLATAGAGDVLAGLVGALLASGLEPTAAGALGALVHGVAADAAR",
    "response#2": "LIVAGDTETMGAALLSVNACLQAGVGLVSACIPQSGLTALNVSLPEAMYFNRENLSNTDLQKYQAIAIGSGLGIGETQEQLLEQLLSKNKPLLIDADALNILSENKELLKEIPAQSVLTPHMKEFDRLFGHHDNWWNRVEMAKKKAIELELIIVLKNQYTFICLPTGDICVNPTGNPAMAQGGMGDVLTGIITSFLAQGYNSSQAVILGVYLHGKAGDILAENREIVTASALAKSLR",
    "response#3": "GVVAGGEDYTGAPILACTAAVSAGVGMVRYVGTPTPEQLVRTLVPEVVLGPGRVQAWVVGPGLDVTSRKAGAKAQVDVAKEALSSDLPVLVDAGGLDLVHGRRSAPTLLTPHAGECARLLTRLARRKRAITPDEVKAAPLHHARRLAALTGASVLLKGSTTLVVSPDPDAPVHSQRDAPPWLATAGAGDVLAGLVGALLASGLEPTAAGALGALVHGVAADAAAVVGGDTGMCGAPILAARAALLAGAGKVHVAFVGSGAPPYDPPHPELMLDAADRLALDAMDALSIGCGMGGSDRARDVLDRALQVAAPTLLDADALNLLARDAALAARVAAHGTPCVLTPHPLEAARLLGADTQAVQRDRPAAARALAARFAAVAVLKGSGTVIAAPDGRLAINPTGNAGLATGGTGDVLGGMIGALLAQKLPPYEAALAGVYLHGLAADTLCAL"
  },
  {
    "reference": "MDRDPLQAEHEANTVRGNRAARIRAEEQSQQKSQSKQGQSGSYKQKLSDSWTGTQIKWYPIPIFLGAVVLVGVQARRNYVAERDGKGVGTKIVDENGQVVKMQGPWTVYVIGALPLNAISRAWGWANNLTLPVWFRPFGFKLYAYIFGCDLDEMKDPDLTHYKSLGEFFYRELKEGAREIDDSVLVSPADGKVLHFGEIMGRRVEQVKGITYSLDALLGVTSAPEATEKVVIDDESEHEKLDDRQFASVNGIDYTLDELLGDEKSKSAVPDPEDAPKRKSKWKRFIKASYWKSLVKKPSTEESNTTGIPPSTAQPDSDSDDDEDDDDDDQASIARSITDSEKKPKKIDDAGMPEPDTPEVLGRYANVAYEMGVSALPPLLQPHSPGAKGVKEGNKLYFCVIYLAPGDYHRFHSPTNWVAERRRHFRGELYSVSPYMAKRLSNLFVLNERVALLGRWRHGFFGMVPVGATNVGSIRINFDKALRTNVRMQRYLAGTYSEASYGGASKLLGGQPLGAGDEMGGFLLGSTIVLVFEAPSDFRFDLKPDQKVKVGQRLGDIRPPTQAEEDEAKKNGRQV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein's Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity, Charge relay system; for autoendoproteolytic cleavage activity must be carefully engineered to ensure that the amino acids involved in a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-   diacyl-sn-glycero-3-phosphoethanolamine + CO2 are properly positioned for optimal catalysis.2. Prioritize Basic and acidic residues, Acidic residues, Polar residues compositional bias within the protein sequence to achieve desired characteristics.3. The protein should be engineered to have a higher affinity for a specific molecule or receptor in mitochondrial inner membrane that is critical for protein autoprocessing, phosphatidylethanolamine biosynthetic process.4. The Mitochondrial matrix topological domain of the protein should be designed to be flexible or rigid.5. The protein must exhibit the following characteristics: Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.; Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer).6. The presence of pyruvate is crucial for the protein to function optimally under specific conditions.7. We require a protein that can specifically bind to the metabolites produced in the phosphatidylethanolamine biosynthesis, Phospholipid metabolism pathway. The designed protein sequence is ",
    "response#1": "MASLGSLNGGGSGGGGGSSGGGSSSPGRHRYKTEPLDPGPAPPPASGPGGGSAGGGGGGAGGGGAGGGGAGAGGGGSAVAGGGGGGSGGGGGGGGGAGGGGGGGGGGGSGAGGGCEGGGAGGVRGARGGGYDGGGGGSGGGGGGSASPGPGPPPPDPYSPRAPSPPDCPEPGDDPRSPPPRKRPRKQDEELELEPEPAPEPAPEPELARGAPAAAGPGGGGGGSGGGGGGSGCGGGGGGGGGGAAAGALPAPPPPPPLGLGVGGGMGAGGGGGGGGGGGAGGGGAPGGGAAAAGGGGAAEGAAGAAAAGEAGGGGGGEAEGAAGGAGGGGGGGAGGGGGGGVGAAAAPPPPQPPPLPPPPPPPPPGAAAAAASPPPPPPPLPPPPPPPLPPPPPPPPPPPLPAPPPPPLLEEAFPPPPPEPAAAAAAAAAAAAAAPAAAAAAAAAAAAAAAAAAATATAAEAPPAPPP",
    "response#2": "MPSTSLHLALLLAAAGVRASHPGSASTARATPHQSPPSAGPSGPPARPPAGNATAAPAPRNASTPHPRAPAPAPPARNATAPPAGNGSAPPANGSGPAPPPPANGSGPAPPPGNASAPAPPGNATAPAPPPANGSGPAPPPANGSGPAPPPANGSGPAPPPANGSGPAPPPPNGSGPAPPPPNGSGPVPPPPNGSGPVPPPPNGSGPAPPQPNGSAPAPPPANGSGPAPPPANGSGPAPAPPQANASAPPPAGNGSAPAPPANGSAPAPPQPNGSAPAPPQANGSAPAPPPANASAPPPPANGTAPAPPVANGTAPAPPANGTAPAPPAVNGTAPAPPATNGTAPAPPQPNGSAPAPPPANGSAPAPPPANGTAPAPPATNGTAPAPPAVNGSAPAPPPANGTAPAPPQPNGSAPAPPQPNGSAPAPPQPNGSAPAPPQPNGSAPAPPQPNGTAPAPPQPNGSAPAPPQPNGSAPAPPQPNGSAPAPPQSNGSAPAPPQPNGSAPAPPQPNGSAPAPLQPNGSAPAPPQANGSAPAPPQPNGSAPAPLQPNGSAPAPPPANGSAPAPPPANGSAPAPPPANGSAPAPPPANGSAPAPPPANGSAPAPPPANGSAPAPPPANGSAPAPAPTNGTAPAPPANGTASAPPPANGTAPAPPTN",
    "response#3": "MNMSTSSPSLPDPGPRSAAAAPSSAAPAAAAPSSSSAAAAASSSAAAASSAAAAAAASAAASSAAAAASSAAAGTTAGGGSGAPGGAGGGGGGGGGSGGVGGGGVGGGAVGGVGGSGGAGGGVGGGVGGVGGGGVGGGVGGGGGAGGVGGGGVGGGGVGGGAGGVGGGGVGGGAGGVGGVGGVGGGGGAGGVGGGGVGGGGVGGGGVGGGGGAGGGAGGVGGGGVGGGGTGAGGGGSGGGGSGGGGGGGGSAGGGAGGGGAGGGGAGGGAGGGGAGGGGTGGGGTGGGGGGPGGGGGGGGGGGAGGGGGGAGGGGGGGGGGGTGGGGTGGGGAGGGAGGGGGGGSGGGGAGGGGAGGGGAGGGGAGGGGAGGGGGGGGGGGAGGGGGGAGGGGGGAGGGGGGCGGGGGGGCGGGGGGCGGGGGGCGGGGGGCGGGGGGCGGGGGGCGGGGGCGGGGGGCGGGGGGCGGGGGGCGGGGGGCGGGGCGGGGGGCGGGGGGCGGGGGGCGGGGGGCGGGGGGCGGGGGGCGGGGGGCGGGGGGCGGGGGGCGGGGGGCGGGCGGGGGGCGGGCGGGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGCGGGC"
  },
  {
    "reference": "MEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEEDAESEDEEEEDVKLLSISGKRSAPGGGSKVPQKKVKLAADEDDDDDDDEDDDDEDDDDDDFDDEEAEEKAPVKKGQESFKKQEKTPKTPKGPSSVEDIKAKMQASIEKGGSLPKVEAKFINYVKNCFRMTDQEAIQDLWQWRKSL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The ideal protein would be one that plays a critical role in positive regulation of cell population proliferation, protein localization, nucleocytoplasmic transport, nucleosome assembly, positive regulation of NF-kappaB transcription factor activity through NF-kappaB binding, molecular condensate scaffold activity, transcription coactivator activity, RNA binding, protein homodimerization activity within spindle pole centrosome, cytoplasm, nucleoplasm, ribonucleoprotein complex, protein-DNA complex.2. The protein design should emphasize Acidic residues, Basic and acidic residues compositional bias.3. The designed protein should contain one or more Nucleoplasmin core, Nucleophosmin C-terminal domains that are essential for its function. The designed protein sequence is ",
    "response#1": "PAPTASLLSIASRKGLVAAAGPDAVHVATTESVRKAFLAEKNGDSEVRPFNPEAKLPLPLRISQLAFTADEQYLVLSAETGGGLRAYDVNSLTQGNTQSAFELATNGETLRQLAPNPMPESAAFCAIVTTNGNLYMANLAERTLVSGPNGPTLRSQVSCAAWSTKGKQLVAGMADGSIYQMTPDGTEKAHIPKPPNLGDYHVSSVVWLENNVFLTIHNPTNSTNPDDKTVYHVITRQQSSGSPPNFTFQKLNDPVEPFVADKTPHHTVLRLKDFPPNLQDLLLVSSTAVETIGLLTRSKTPLATDKPADAITNVFTTTELADDSRRAQLPMSEDMMETYPIGVALDLSSKEKVYKPIPTDEEIEYSPGPLPGLWVLNNEGVL",
    "response#2": "PAPTASLLSIASRKGLVAAAGPDAVHVATTESVRKAFLAEKNGDSEVRPFNPEAKLPLPLRISQLAFTADEQYLVLSAETGGGLRAYDVNSLTQGNTQSAFELATNGETLRQLAPNPMPESAAFCAIVTTNGNLYMANLAERTLVSGPNGPTLRSQVSCAAWSTKGKQLVAGMADGSIYQMTPDGTEKAHIPKPPNLGDYHVSSVVWLENNVFLTIHNPTNSTNPDDKTVYHVITRQQSSGSPPNFTFQKLNDPVEPFVADKTPHHTVLRLKDFPPNLQDLLLVSSTAVETIGLLTRSKTPLATDKPADAITNVFTTTELADDSRRAQLPMSEDMMETYPIGVALDLSSKEKVYKPIPTDEEIEYSPGPLPGLWVLNNEGVPAPTASLLSIASRKGLVAAAGPDAVHVATTESVRKAFLAEKNGDSEVRPFNPEAKLPLPLRISQLAFTADEQYLVLSAETGGGLRAYDVNSLTQGNTQSAFELATNGETLRQLAPNPMPESAAFCAIVTTNGNLYMANLAERTLVSGPNGPTLRSQVSCAAWSTKGKQLVAGMADGSIYQMTPDGTEKAHIPKPPNLGDYHVSSVVWLENNVFLTIHNPTNSTNPDDKTVYHVITRQQSSGSPPNFTFQKLNDPVEPFVADKTPHHTVLRLKDFPPNLQDLLLVSSTAVETIGLLTRSKTPLATDKPADAITNVFTTTELADDSRRAQLPMSEDMMETYPIGVALDLSSKEKVYKPIPTDEEIEYSPGPLPGLWVLNNEGV",
    "response#3": "PAPTASLLSIASRKGLVAAAGPDAVHVATTESVRKAFLAEKNGDSEVRPFNPEAKLPLPLRISQLAFTADEQYLVLSAETGGGLRAYDVNSLTQGNTQSAFELATNGETLRQLAPNPMPESAAFCAIVTTNGNLYMANLAERTLVSGPNGPTLRSQVSCAAWSTKGKQLVAGMADGSIYQMTPDGTEKAHIPKPPNLGDYHVSSVVWLENNVFLTIHNPTNSTNPDDKTVYHVITRQQSSGSPPNFTFQKLNDPVEPFVADKTPHHTVLRLKDFPPNLQDLLLVSSTAVETIGLLTRSKTPLATDKPADAITNVFTTTELADDSRRAQLPMSEDMMETYPIGVALDLSSKEKVYKPIPTDEEIEYSPGPLPGLWVLNNEGVL"
  },
  {
    "reference": "MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRALGPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGIDPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITYGIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQFNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRALSRAVAQKYVESREKLGFPLMKANAA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a specific function within the cytoplasm.2. The protein should be designed to have high stability and activity towards ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-   tRNA(Gly). The designed protein sequence is ",
    "response#1": "TFKRAANIIRKQGGDSDVALDGAWKADLLVEDAERHLAASLEAMFPRFDGLWAEGDYPALFGLLGELRPVVDGFFEGVMVMSDDAALRTNRLNLLQAT",
    "response#2": "TFKRAANIIRKQGGDSDVALDGAWKADLLVEDAERHLAASLEAMFPRFDGLWAEGDYPALFGLLGELRPVVDGFFEGVMVMSDDAALRTNRLNLLQAQ",
    "response#3": "VIDSKLFKSESELAFYNALVELVPQTEISQKTRNYQQLVDALLKIAPTVSNFFDGPDSVLVIDPDPKIKKNRLNLLGLLRR"
  },
  {
    "reference": "MEAVSRVFPALAGQAPEEQGEIIKVKVKEEDHTWDQESALRRNLSYTRELSRQRFRQFCYQETPGPREALSQLRELCRQWLNPEIHTKEQILELLVLEQFLTILPEELQSWVREHNPESGEEVVTLLEDLERELDEPRQQVSQGTYGQEVSMEEMIPLDSAKESLGTQLQSMEDRMECESPEPHPLQDNGSFLWFSMMSQSMGGDNLSSLDTNEAEIEPENMREKFFRSLARLLENKSNNTKIFSKAKYCQLIKEVKEAKAKAKKESVDYRRLARFDVILVQGNEKLIEAVNGETDKIRYYLHSEDLFDILHNTHLSIGHGGRTRMEKELQAKYKNITKEVIMLYLTLCKPCQQKNSKLKKVLTSKSIKEVSSRCQVDLIDMQLNPDGEYRFILHYQDLCTKLTFLRSLKSKRPTEVAHALLDIFTIIGAPSVLQSDNGREFSSQVVSELSNIWPELKIVHGKSQTCQSQSSAEQTEDIRKRIFSWMQTNNSSHWTEFLWFIQMSQNQPYHRSMQQTPCESAFSSEAKLGLSHSQLTEELVASLHTENELDQADKELENTLRAQYEENIETGTDSSDIEENLSVTPKVAEKSPPESRLRFLSCVVCEKECTGVNSCISCDGNIHAICGVPSQHGTEGCGRQITCSLCYETSTMKRKHDEIQRSLPVKPSKMLKPSGTPFSPDKVGDWMAKQASLDFFVKKRHAFSEHSSSNKRNVNNRSYPEEGKTKRVHASFTRKYDPSYIEFGFVAVIDGEVLKPQCIICGDVLAN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be involved in positive regulation of cell cycle, positive regulation of epithelial cell proliferation, DNA integration by localizing to nucleus, cytoplasm and exhibiting nucleic acid binding.2. The presence of SCAN box, Integrase catalytic domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MYSPASILDIKQGPKETFRDYVDRFYKTLRAEQATQEVKNWMTETLLVQNANPDCKNILRALGPGASLEEMMTY",
    "response#2": "MNPLQLLQPLPAEIKGTKLLAHWDSGATITCIPESFLEDEQPIKKTLIKTIHGEKQQNVYYVTFKVKGRKVEAEVIASPYEYILLSPTDVPWLTQQPLQLTILVPLQEYQEKILSKTALPEDQKQQLKTLFVKYDNLWQHWES",
    "response#3": "MYSPASILDIKQGPKETFRDYVDRFYKTLRAEQATQEVKNWMTETLLVQNANPDCKNILRALGPGASLEEMMTR"
  },
  {
    "reference": "MGDPKRPKKKYVEGKPKRLWNSDLLMSELRLIGEYGLRNKKELWLARALLRSIKHRAREILSAPMEIRGEAEKRLKDRLFRMGLISDINIPLDSVLALDVTAVLERRLQTLVYRKGMARSIHEARQLIVHGHISINGVRVRSPGYLVPRNLEDGIGFAPTSRIVKAKVTQQVNQGGQAVNQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that localizes to small ribosomal subunit and can perform structural constituent of ribosome with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "MRLDNTIFRLGMAATIRAARQLVNHGHIVLNERVVDIPSYRCKPNDKITIRDRDRSRILIEKNL",
    "response#2": "MRLDNTIFRLGMAATIRAARQLVNHGHIVLNERVVDIPSYRCKPNDKITIRDRDRSRILIEKNL",
    "response#3": "KYIDLMSIYLPHTGGRHEHRRFGKSRIPIVERLINNLMRPGRNKGKKMLAYNIVKTTFDIIAVKTGQNPIQVLVRAIENAAPREEVTRIMYGGIVYYVAVDVAPQRRVDLALRHLVTGASEASFNNPKPIEEALAEEIIAAANNDNKSVAIRKKEEIERIALSSR"
  },
  {
    "reference": "MNIAVVGLSHKTAPVEVREKLSIPEPQIESAIAQLASYPHIDEVAILSTCNRLEIYIVTSEADQGIREITQFLAEYSKLPVLSLRQHLFMLLHDDAVMHVMRVAGGLDSLVLGEGQILAQVKTTHKLGQQYNGIKTILNRLFKQALTAGKRVRTETSIGTGAVSISSAAVELAQIKVANLAACRVVILGAGKMSRLLVQHLISKGAVEISIVNRSRDRAQELAKLFPQQPIKIHPLSEMMSVIADSDLVFTSTSATEPILDRVKLEMVLEVQRSLMLFDISVPRNVHADVNELENVQAFNVDDLKAVVAQNYESRRKIAQEAEKLLEEEVEAFDIWWRSLETVTTISCLRNKVETIREQELEKALSRLGSEFAEKHQEVIEALTRGIVNKILHDPMVQLRSQQDVEARRRCMQTLQMLFNLDAEEQFS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must have a Nucleophile active site that is highly conserved among related enzymes.2. The protein design should be able to enhance the efficiency of chlorophyll biosynthetic process. The designed protein sequence is ",
    "response#1": "LVSASQMLLPDSDGAIEGHIREEGLTVHLKKDVPALFSANIDTPLVEAFKPLGISDWNSIFWIAHPGGPAILDQIEEKLGLKEDKLRASKHVMSEYGNMSSSCVLFVLDEMRSRSLQDGKSTTGEGLDWGVLFGFGPGLTVETVVLRSVD",
    "response#2": "LVSASQMLLPDSDGAIEGHIREEGLTVHLKKDVPALFSANIDTPLVEAFKPLGISDWNSIFWIAHPGGPAILDQIEEKLGLKEDKLRASKHVMSEYGNMSSSCVLFVLDEMRSRSLQDGKSTTGEGLDWGVLFGFGPGLTVETVVLRSVR",
    "response#3": "LVSASQMLLPDSDGAIEGHIREEGLTVHLKKDVPALFSANIDTPLVEAFKPLGISDWNSIFWIAHPGGPAILDQIEEKLGLKEDKLRASKHVMSEYGNMSSSCVLFVLDEMRSRSLQDGKSTTGEGLDWGVLFGFGPGLTVETVVLRSVS"
  },
  {
    "reference": "MSSGRTIVVGITGGIAAYKAVNVVRAFVLRGDCVHVVATPAALRFVGKPTLEAISRNPVHTDLYEGVAEVRHVAIGQSADLIVVAPATANTIAKLATGLADDLLGNTILASTAPLVIAPAMHTEMWNNAATVANVATLRARGVIVVGPGVGQLTGADAGAGRMEEPDTIVAAALAALALADRTDGPGPDLVGKHVVITAGGTREPLDPVRFLGNRSSGKQGVALAQAALARGASVTLIAANLEVAAPTAVELRLVGTALELEAAVTDAAAGADAVIMAAAVADYRPVQVSAGKIKKDETGDTLLLELTRNPDILAGLARTKRTGQVLIGFAAETEPDPVRLLVLGREKMARKGCDFLVVNRVGWAEGFASDRNAITVLDHDGDIVCEVSGTKMSVAGRILDLIG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.2. We are looking for a protein with broad substrate specificity for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.3. The protein should be optimized for activity in Cofactor biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The designed protein should be optimized for phosphopantothenate--cysteine ligase activity activity in order to improve pantothenate catabolic process, coenzyme A biosynthetic process.5. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.6. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.. The designed protein sequence is ",
    "response#1": "MKVLLGVCGGIAAYKAPELVRHFQKAGHQVAVALTPAATRFVSPLVLRTLADGGVLEGIFSTEERVAHVALADWADVVAVAPATAHTLARLATGMADEPVSLCFLATRARRIVFPAMNVNMWINPATQANVACLRQRGVEVVEPESGRLAGVAQAGGLAGLHVLVSAGGTREPIDAVRFVGNRSSGKMGYALAARAAARGATVTVVAANVALPDPPGATVVRVGTAEDLRRACGDAFGSADVLLMAAAVADFRPVDPAGGKIKKAGRTELTIELEPTVDVLTELSGRRRDGQTLVGFAAEHGDGALDYARDKRERKRLDAVVLNDVAVPGIAFDAADNAVTILTPGAAPVEVARGSKREVADAVLDLVAARLG",
    "response#2": "MKVLLGVCGGIAAYKAPELVRHFQKAGHQVAVALTPAATRFVSPLVLRTLADGGVLEGIFSTEERVAHVALADWADVVAVAPATAHTLARLATGMADEPVSLCFLATRARRIVFPAMNVNMWINPATQANVACLRQRGVEVVEPESGRLAAAVGAGRSGVRVLVTAGPTREYLDPVRFLSNPSTGTMGFAIAARAASRGAEVTLVSGPVALPTPAGVRRIDVVSAADMREAVFAELAARPAQVVIAAAAVADYRPLQRASQKLKKGEARRSVELERTEDVLAELVRSFHPPVRVGFAAETENILANARDKLLRKDLSLVVANDVSAGSGAFGDALTTVWLFTRDGAVEELPVMPKLEVAERLFDRVVALRSETAGAR",
    "response#3": "MKVLLGVCGGIAAYKAPELVRHFQKAGHQVAVALTPAATRFVSPLVLRTLADGGVLEGIFSTEERVAHVALADWADVVAVAPATAHTLARLATGMADEPVSLCFLATRARRIVFPAMNVNMWINPATQANVACLRQRGVEVVEPESGRLTGPQTGEGRLLTGKHVVISAGGTREPLDPVRYLGNHSSGKQGYALAATAAARGARVTLLSANATLPDPAGVDVVHVSTALELREAALKACADADAVVMAAAVADFRPAEYATGKIKKKDGVDPAPVALVRNPDILAELSAHRARPGQLVVGFAAETDEVLANGRAKLARKGCDLLVVNEVGGGKAFGQDVNAAVLLAADGSETRVPLGPKEALADTVWDLVVARLGG"
  },
  {
    "reference": "MAGWGLLLLLLWVVSLAQGSSLFSGQRHRTRLQRDRQARNNVRPNIIIILTDDQDIELGSMQAMNKTRRIMEQGGTHFSNAFSTTPMCCPSRSSILTGKYVHNHHTFTNNENCSSPSWQAHHEPHTFAVHLNNSGYRTALFGKYLNEYNGSYVPPGWREWVALVKNSRFYNYTLCRNSVREKHGSQYPKDYLTDIITNDSINYFRSSKRMYPHRPVMMVLSHAAPHGPEDSAPQYSTAFQNASQHITPSYNYAPNPDKHWILRYTGPMKPVHMQFTNMLQRRRMQTLLSVDDSVDKVYNMLVETGELDNTYIIYTSDHGYHIGQFGLVKGKSMPYEFDIRVPFFIRGPNVEAGAINPHVVLNIDLAPTLLDMAGLDVPSDMDGKSILKLLDTDKPINRFQVNKKGKMWRDSFLVERGSLLHKRSDGKDLAQEENFLPKYQRVKDLCQRAQYQSSCEQPGQKWQCVEDPSGKLSLYKCKGMASLYASRMQALMASSGSQQWVGRVSDAGDKVKSGKSLTKNRWARSVPFELHGDMYAVDLVEGYRPVGLRNTSWPGERRRGAGLLEDNDEEFSGNANCGKCSCQVKMRMAGVCLPHRCSILMNDTVRCDGGLYKSLQAWKDHKLHIEHEIETLQTKIKNLREVKGHLKKVRPEECQCNTPSYLSKNKGMFRLNAGRIYSLSKMPSKQKKQWLMKEQKRRKKLRKLLKRLRNNDTCSMPGLTCFTHDNQHWQTAPFWTMGPFCACTSANNNTYWCLRTINDTHNFIFCEF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a specific function in negative regulation of fibroblast growth factor receptor signaling pathway and should be localized to Golgi stack in order to carry out this function efficiently.2. The designed protein must have at least one Extracellular sulfatase C-terminal domain. The designed protein sequence is ",
    "response#1": "SQFLRKRWARSISFELGGDLYAVDLEKGYWPLGSRNSSWARDRRRGVNEEDNEEFSGMEVTVKPTTSNKLAPPAAIKVTYRCSILMNDTVKCDGGLYKSLQAWKDHKLHIEHEIETLQTKIKNLREVKGHLKNVRPEECQCQ",
    "response#2": "SQFLRKRWARSISFELGGDLYAVDLEKGYWPLGSRNSSWARDRRRGVNEEDNEEFSGMEVTVKPTTSNKLAPPAAIKVTYRCSILMNDTVKCDGGLYKSLQAWKDHKLHIEHEIETLQTKIKNLREVKGHLKNVRPEECQCG",
    "response#3": "SQFLRKRWARSISFELGGDLYAVDLEKGYWPLGSRNSSWARDRRRGVNEEDNEEFSGMEVTVKPTTSNKLAPPAAIKVTYRCSILMNDTVKCDGGLYKSLQAWKDHKLHIEHEIETLQTKIKNLREVKGHLKNVRPEECQCS"
  },
  {
    "reference": "MDGLMSSADEILDIVDENDRVVGQARRGDAYARGLRHRCVFVWARDPEGRVFVHRRTATKLVFPAPYDMFVGGVVGAGESYDDAALREAEEELGASGLPRPEFLFKFLYDDGAGRTWWSAVYEVRVAGAVSPQVEEVAWHGFLPEAELEGRLGEWEFVPDGLSAYARLRAWRGASGPGSVG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The presence of Mg(2+) is necessary for the protein to function in the desired environment.2. The designed protein must be optimized for hydrolase activity, acting on acid anhydrides, metal ion binding.3. The protein design must feature the Nudix box motif.4. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "FLEKVDRCNRGLEKLGEFIPFVIEEQIVGYIHKGFTKYLRDFNDIFTFSQYGGHVTLNMMLDKPEERTRAVAHVIKILGNKGIIPGIRNELYPVKPSFNAPAFFSIERAAAPYFGLF",
    "response#2": "FLLPVPMMNIINGGAHADNSIDMQEFMILPVGAPSFREALRYGAEVFHALKKVLSDRGLATGVGDEGGFAPNLPSNEAAIGIILEAIEKAGYRPGEDICLGLDVASSEFYSDGIYTLASEGRRFTSEEFSDHLAAWVDKYPIVSIEDGMAENDWHGWGIHTDRLGRRIQLVGDDLFVTNPAILKQGIEARIANSILIKVNQIGTLTETLEAIHIARKAGYTSVVSHRSGETEGTTIADIAVATCTGQIKTGSLSRSDRIAKYNRLLKIEEELGDKARYGGRGAVV",
    "response#3": "LEVLMVSSPNRHDLVFPKGGWEDDETVLEAASREAIEEAGVKGILRELPLGVWEFRSKSSTVEDECLGGCKGYMFALKVTEELEDWPERKNRERRWLTVKEALELCRYEWMQRALE"
  },
  {
    "reference": "MYKLLIINP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have a fast turnover rate for ATP + butanoate = ADP + butanoyl phosphate.2. A protein that can perform ATP binding, butyrate kinase activity in cytoplasm to support phosphorylation, butyrate metabolic process.3. For general function, the protein need meet that Catalyzes the conversion of butyryl-CoA through butyryl phosphate to butyrate.4. The designed protein should interact with enzymes and substrates within the Lipid metabolism, butanoate metabolism pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "LERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHLGISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEPQLLETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEWDPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTTSVYLWSVPMFHCNGWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPEERYALKARQGLNHLAMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYYRRPEETAEVLKNGRLY",
    "response#2": "KATLEAVKLVSSGHADMLMKGLVDTATFLRSVLNKEVGLRTGKLMSHVAVFDVEGWDRLLFLTDAAFNTYPEFKDKVGMINNAVVVAHACGIDVPRVAPICPVEVVNTSMQSTVDAALLAKMSDRGQIKGCVIDGPFALDNAISEEAAHHKGVTGSVAGKADILLLPNIEAANVMYKTLTYFSKSRNGGLLVGTSAPVILTSRADSFETKVNSIALAH",
    "response#3": "LERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHLGISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEPQLLETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEWDPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTTSVYLWSVPMFHCNGWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPEERYALKARQGLNHLAMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYWGRPEETASVLRDGWLYTGDIGYLDDEGFLYVVDRSKDMIISGGENIYPAEVEKVLYMYQEVLEAAVVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILRADLVKWATEQE"
  },
  {
    "reference": "MPDKRAVYAFSGDPITYGHVDIVQRVASTYTYVCVAIGDNPEKAGRYLFSREERLAMTQKALAHLPNVECTIMEGLLAEYAFRHAFDVIVRGVRNNSDLEAELSFFAVNTSLHPSLDTILIPARPQLSHISSSVVKALVLEGGDVSKFCPLFVKQKLERKLLGKLFIGIAGGIAAGKSTIGRMLQQKISEKVEATLLSLDTIGHEILGDSSEILYQKTRQSIIRTFGSEVANPDGTINRTRLGQIVFADAAALQRLNAIMRDPMLARLYEHIRRSQAGIVLLEGAILVEANWTQLVNNNVILVDAPVPQRLQRIVDRNHIPREEALRKIERQPDHAQRVQMLQEKISHAGWGHCWECDNSDGRAEMEVSKIASEIIHLWETDPPR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Ideal protein should have a binding affinity for key enzymes in the coenzyme A biosynthesis, CoA from (R)- pantothenate: step 5/5, Cofactor biosynthesis pathway to enhance the overall efficiency of the pathway.2. The protein should be designed to catalyze the following reaction 3'-dephospho-CoA + ATP = ADP + CoA + H(+).3. A protein that contributes to coenzyme A biosynthetic process, phosphorylation by functioning in cytoplasm with ATP binding, dephospho-CoA kinase activity, pantetheine-phosphate adenylyltransferase activity.4. The protein must exhibit the following characteristics: Reversibly transfers an adenylyl group from ATP to 4'- phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate..5. The protein must bind to Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "RVVLGVTGGIAAYKACELLRRFTEAGHAVRVVPTASALRFVGAPTWSALSGQPVSTEVWDDVHEVPHVRIGQQADLVVVAPATADVLAKAAHGLADDLLTNTLLTARCPVVFAPAMHTEMWEHPATRENVATLRRRGAVVIEPAVGRLTGADSGKGRLPDPGEIVRLAAHALGRPLAGRHVVVSAGGTREPLDPVRFLGNRSSGKQGYALARTAAARGARVTLLSANAALPDPAGVDVVHVTTARELRDAVRQAAGTADAVVMAAAVADFRPAAYATGKIKKQEGKEPEPVALVRNPDILAELAADRARPGQLIVGFAAETDDILANGRAKLRRKGCDLLVVNEVGENKTFGAEESEAVVLGADGSETPVPYGPKEALADVVWDLVAERLG",
    "response#2": "RVVLGVTGGIAAYKACELLRRFTEAGHAVRVVPTASALRFVGAPTWSALSGQPVSTEVWDDVHEVPHVRIGQQADLVVVAPATADVLAKAAHGLADDLLTNTLLTARCPVVFAPAMHTEMWEHPATRENVATLRRRGAVVIEPAVGRLTGADSGKGRLPDPGEILAVAAAALGTGAPGLAGRHVVVSAGGTREPLDPVRFLGNRSSGKQGYALARTAAARGARVTLLSANAALPDPAGVDVVHVTTARELRDAVRQAAGTADAVVMAAAVADFRPAAYATGKIKKQEGKEPEPVALVRNPDILAELAADRARPGQLIVGFAAETDDILANGRAKLRRKGCDLLVVNEVGENKTFGAEESEAVVLGADGSREELPRADKRALADRVWDLVAARL",
    "response#3": "RVVLGVTGGIAAYKACELLRRFTEAGHAVRVVPTASALRFVGAPTWSALSGQPVSTEVWDDVHEVPHVRIGQQADLVVVAPATADVLAKAAHGLADDLLTNTLLTARCPVVFAPAMHTEMWEHPATRENVATLRRRGAVVIEPAVGRLTGADSGKGRLPDPGEILAAAVRVLEGGAPVDLAGRHVVVSAGGTREPLDPVRFLGNRSSGKQGYALARTAAARGARVTLLSANAALPDPAGVDVVHVTTARELRDAVRQAAGTADAVVMAAAVADFRPAAYATGKIKKQEGKEPEPVALVRNPDILAELAADRARPGQLIVGFAAETDDILANGRAKLRRKGCDLLVVNEVGENKTFGAEESEAVVLGADGTATELAHGSKTLMASRVLDAVAARLG"
  },
  {
    "reference": "MSTNPKPQRKTHRNTNRRPMDVKFPGGGQIVGGVYLLPRRGPRLGVRATRKTSERSQPRGRRQPIPKARRSEGRSWAQPGYPWPLYGNEGCGWAGWLLSPRGSRPSWGPNDPRRRSRNLGKVIDTLTCGFADLMGYIPLVGAPVGGVARALAHGVRALEDGINYATGNLPGCSFSIFLLALLSCLTVPASAIHYRNSSGVYHITNDCPNSSIIYEADHHILHLPGCVPCVRRGNVSQCWVSLTPTVAAPYLNAPLQSLRRHVDLMVGAATMCSALYIGDLCGGLFLVGQAFTFQPRRHWTTQDCNCSIYLGHITGHRMAWDMMMNWSPTTTLILAQIMRIPQVILDLLAGGHWGVLVGVAYFSMQANWAKVILVLFLFAGVDAETVVTGGRVAATTSGFTKLFSPGASQNLQLVNTNGSWHINRTALNCNDSLNTGFLAGLFYVHKFNSSGCPERLASCKRLDDFLQGWGTLGVANTSGSSEDRPYCWHYAPRQCGVVPASTVCGPVYCFTPSPVVVGTTDRRGVPTYTWGENETDVFVLNSTRPPGGAWFGCVWMNGTGFTKTCGVPPCEIRTANKTWSCPTDCFRKHPETTYSKCGSGPWLTPRCLVDYPYRLWHYPCTVNYTIFKIRMYVGGIEHRLDAACNWTRGEACALEHRDRTEISPLLLSTTQWQVLPCSFTTLPALSTGLIHLHQNIVDVQYLYGVGSAVVSWALKWEYIVLVFLLLADARVCACLWMMLLVAQVEAALTNLITINAMSAAGTHGVIYA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have at least one RdRp catalytic domain.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in lipid droplet that is critical for fusion of virus membrane with host endosome membrane.3. For general function, the protein need meet that RNA-dependent RNA polymerase that performs primer-template recognition and RNA synthesis during viral replication.4. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation.5. The protein should have a fast turnover rate for a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-   diphosphate + H(+) + phosphate. The designed protein sequence is ",
    "response#1": "LSELCQVDTIVPINHTTSTTSSVNMYTIQLTKEQPGTEVFAMRVDVASQPLATTLIGEISSYYTNWTGSLRFSLMFCGSAGTTLKMLVAYTPPGIAKPETRKDAMLGTHVVWDVGLQSTMSMIVPWISASXXXXTTPDTFSQAGYITCWYQTSLVTPPSTPETARMLCFVSGCKDFCLRMARDTC",
    "response#2": "LSELCQVDTIVPINHTTSTTSSVNMYTIQLTKEQPGTEVFAMRVDVASQPLATTLIGEISSYYTNWTGSLRFSLMFCGSAGTTLKMLVAYTPPGIAKPETRKDAMLGTHVVWDVGLQSTMSMIVPWISASXXXXTTPDTFSQAGYITCWYQTSLVTPPSTPETARMLCFVSGCKDFCLRMARDTC",
    "response#3": "VTGGQAGSMTRHVTGLFTPGARQNLQLVNSNGSWHINRTALNCNDSLNTGFLAGLFYTHKFNSSGCPERLASCKPLGGFPQGWGTLGVANNNGSSEDRPYCWHYPPRQCGVVSASTVCGPVYCFTPSPVVVGTTDRRGVPTYTWGENETDVFILNSTRPPGGAWFGCVWMNGTGFTKTCGAPPCVVRTSNNTWQCPTDCFRKHPETTYLKCGSGPWLTPRCLVDYPYRLWHYPCTINYTIFKIRMYVGGIEHRLNAACNWTRGEACDLEHRDRAEISPLLLSTTQWQVLPCSFTTLPALSTGLIHLHQNIVDVQYLYGVGSAVVSWALKWEYVVLAFLLLADHVTGAVAGRTVGNIASLFSPGSRQNLQLINSNGSWHINRTALNCNDSLQTGFIASLFYFNKFNASGCPDRMSSCKPLTYFDQGWGPISYANVSGSSEDKPYCWHYPPRPCGVVPASQVCGPVYCFTPSPVVVGTTDKKGLPTYTWGENESDVFLLESLRPPKGGWYGCTWMNSTGYVKTCGAPPCNIKPDASSSNTTLTCPTDCFRKHPEATYTRCGSGPWLTPRCLVDYPYRLWHYPCTQNYTIHKVRMFVGGLEHRFQAACNWTRGAPCNLDDRDRVEMSPLLFSTTELAILPCSFTTMPALSTGLIHLHQNIVDVQYLYGISPSITSWVIKWEYIVLAFLLLADARVC"
  },
  {
    "reference": "MGRAYQNRKDSIAKTANAKSKVYSRYGREIYVCAKQGGGDPDGNLALRGLIDRAKKDQVPAHVIEKAIDKAQGGAGEDFSPARYEGYGPSNCMVIVECLTDNPNRTFGDVRNCFTKSKSKIGAQGSVAHSFDHCAIFAFRHDNEDEVLEAMMEADVDVTDVEFEDGLVTVFAPNTEYAAARQALTHAFPDIDFEADEIQFLPQVTTSIAEDDLPMFERLMDMLNDLDDVQNIYHNAEH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must have high specificity towards DNA binding. The designed protein sequence is ",
    "response#1": "VEDHLQRYLAEQIERIGKGAKLVRREYPTAIGPVDIMAVNAEGEHVAVEIKRHGGIDGVEQLTRYCELLNRDPLLAPVHGIFAAQTITPQAQVLAKDRGFTCLILDYDDMKGTEDDSLRLG",
    "response#2": "AGVDWASESHHVFLTDGDGRKIGERVFRHGGEGLAEMAAWLMATSGAVEGDEIQVAIEVPHGPVVETLIERGFKVNAINPKQMDRFRDRFTMAGAKDDSRDAEVMASALRTDPRCFRLLAVSDPVVIELREWSRIAEDLSAERNRLTNRMREQLWRYFR",
    "response#3": "AGVDWASESHHVFLTDGDGRKIGERVFRHGGEGLAEMAAWLMATSGAVEGDEIQVAIEVPHGPVVETLIERGFKVNAINPKQMDRFRDRFTMAGAKDDSRDAEVMASALRTDPRCFRLLAVSDPVVIELREWSRIAEDLSAERNRLTNRMREQLWRYFG"
  },
  {
    "reference": "MPDLLTCDRHQIELSLAPIPLSAAAEFCHDDRYGAFASFVGWVRRVNVGRLVTGITYQSFQPLCRTVLTEICQEAEQVFGQELRIYVQHRLGETRVGDPTVLIGVGAIHRDEACEACRYVIEELKHRAPIWKLEHYEDGDSGWVPGNCLCQERRSRDRRGSTGPE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The substrate binding site should be stable and able to withstand changes in environmental conditions.2. The protein must exhibit the following characteristics: This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group; The sulfur is provided by MoaD .; Converts molybdopterin precursor Z into molybdopterin.3. A protein that is capable of catalyzing reactions within the molybdopterin biosynthesis, Cofactor biosynthesis pathway with high efficiency and specificity.4. The desired protein should be able to perform 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal   Gly-NH-CH2-C(O)SH + cyclic pyranopterin phosphate + H2O = 2   [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly +   4 H(+) + molybdopterin5. The designed protein must possess molybdopterin synthase activity to support Mo-molybdopterin cofactor biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "LRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELETRLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQMRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFASVPYCIQLSQGDAGTRLLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELETRLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQMRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFASVPYCIQLSQGDAGTRLTGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELETRLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQMRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNR",
    "response#2": "LRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELETRLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQMRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGLVTLVRPGGGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELETRLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQMRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNEAQ",
    "response#3": "LRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELETRLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQMRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFASVPYCIQLGMQGDLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELETRLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQMRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGELVSISDRASSGVYQDKGIPALEEWLTSALTTPFELETRLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQMRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGA"
  },
  {
    "reference": "MNRIIRTSGFYLLIFLVTVGIVQFISSQNDSSAEIRYDEFRTALQTDNVASLELQFDGYSYLVTGEYKKPPEANKKQFYTRTNVDEFVTKEIDDAAQKNHFELEYKKMKGESMWLTLLTSIIPFVIIFILFFFLMNQAQGGGGKVMNFGKSRARLYNEEKKRVTFEDVAGADEEKQELVEVVEFLKDPRKFAAVGARIPKGVLLVGPPGTGKTLLARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFENAKKNAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFGANEGIIIVAATNRPDILDPALLRPGRFDRQITVDRPDVKGREAVLKVHARNKPLSKDVKLDIIAKRTTGFTGADLENLLNEAALLAARRNKKDIAMVEVDEAIDRVIVGTEKRSRVISDREKRIVAYHEAGHTIVGYFLEHADMVHKVTIIPRGKAGGYVIMMPKEDRMLVTKQELLDKVTGLLGGRVAEEIFIGEVGTGAYSDFKQATSIVRSMIMEYGMSDKLGPMQFGSSQGQVFLGRDIGHEQNYSDKIAYEIDQEMQFFINECYDRAKKLLTEKSKEMHLIAQTLLSEETLELDQIKRLIETGSLTGELPEGESTASTEEFRSDVKVDTIGGVKVRIQTKDSEESPTPVSDTTDKAGDDDGSGNPGTDKNE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The presence of AAA+ ATPase domains is critical for the interaction of the designed protein with its intended targets.2. The protein should have a high affinity for Zn(2+).3. A protein with metalloendopeptidase activity, ATP hydrolysis activity is needed to enhance proteolysis in a specific context.4. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "IPKGVLLYGPPGTGKTLLARAVAGEAGVPFYTISGSDFVEMFVGVGASRVRDLFEQAKQNAPCIIFVDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFDARGGIILIAATNRPDILDPALLRPGRFDRQIPVSAPGFEEDVEFRGIARGTPGFSGADLETLLNEAALVAAKMGKVAVEMTDLETARDKILYA",
    "response#2": "LLVGPPGTGKTLLAKAVAGEADVPFFSMAGSDFVEMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAIGRARSKNGGFSGNDERENTLNQLLTEMDGFGTNSGVIILAATNRVDILDKALMRAGRFDRQITVLPAA",
    "response#3": "LLVGPPGTGKTLLAKAVAGEADVPFFSMAGSDFVEMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAIGRARSKNGGFSGNDERENTLNQLLTEMDGFGTNSGVIILAATNRVDILDKALMRAGRFDRQITVLWFE"
  },
  {
    "reference": "MGGKWSKSSIIGWPAIRERMRRTRPAAEETRPAAEGVGAASQDLGKHGASTSSNTAQXNSACAWPEAQEEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIWSPKRQEILDLWVYNTQGYLPDWQNYTPGPGIRYPLTFGWCFELVPVDPKEVEEXTEGENNCLLHPICQHGTEDPEREVLKWRFNSRLAFVHKARELHPEYYK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a specific function in suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II and should be localized to host cell plasma membrane, host cell Golgi membrane in order to carry out this function efficiently.2. The protein design must feature the PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif.3. The protein must exhibit the following characteristics: Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. The designed protein sequence is ",
    "response#1": "MGGKWSKMSGWPTVRERMRRTEPAAEGVGAASRDLERHGAITSSNTPATNADCAWLEAQEEEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPEKVEEANEGENNSLLHPMSLHGMDDPEKEVLEWRFDSRLAFHHVAREKHPEYYKNC",
    "response#2": "MGGKWSKSSIVGWPAVR",
    "response#3": "MGGKWSKSSLVGWPAIRERMRRAEPAAEGVGAASRDLEKHGAITSSNTAANNADCAWLEAQEEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQRRQDILDLWVYHTQGYFPDWHNYTPGPGIRYPLTFGWCFKLVPVEPEKVEEANEGENNSLLHPMSLHGMEDPEKEVLQWKFDSRLAFHHMARELHPEYYKDC"
  },
  {
    "reference": "MNNLFFSKILRSDNIHFVGIGGVGMGALAEICIKLGYKISGSDIKENLITKKLSVLGAKIYNQHCANNIIESTIIVVSSAIKEDNLEIIYAKKLNIPVVKRAEFLSELMRFKYGIIVSGTHGKTTTTSIISYIYKEAKLFPTYLYGGRLNNSDDYGNLGNSKYWISEADESDNSFLFFRPIISVVTNIEKDHLESYQGNFKKLKESFIKFLHNIPFYGYSVLCLDDIEIKKIIPLINKKIVTYGFNRNSDFCITKFFQSNKKIIFIVYIKHQKRYLKFVSNLLGKHNALNITAAITIAIKEGIKYNIILNSIKKFPGINRRFDFINKYDLSNLNNKKGTITIVDDYGHHPTELKLTIKSVKKIWNKRRLIMIFQPHKYTRTKYLYNYFVKVLSTVDILLIMEIYSAGENPIYKINSKNLCQSIMALKRSHVIFIPNEKFLFKKLELLLKNNDILLLQGAGTIENIFKKLTSKLLL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have ATP binding within cytoplasm to regulate cell division, regulation of cell shape, cell cycle, cell wall organization, peptidoglycan biosynthetic process.2. The protein have enhanced ATP + L-alanine + UDP-N-acetyl-alpha-D-muramate = ADP + H(+) +   phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine.3. The protein should be optimized for activity in Cell wall biogenesis, peptidoglycan biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The designed protein should have a high specificity for ATP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "IAGTHGKTTTTAMVAMIYAQAGLDPTFVNGGLVKSAGTNAYLGCSRYLIAEADESDASFLHLQPMVSVVTNIEPEHMETYHGDFEEMKQTYVNFLRNLPFYGLSVMCADDPVLMELSSQVGRQVVTYGFSDEADYRIEDYQQTGFQGHYTVVAPNGERIDVLLNVPGKHNALNATAALTVATLLGVPLEDIVSVLRDFGGVKRRFEVRGEAHGIRFVDDYAHHPSEIRATLAAGRLQLKSREEFISPWQRLVAVFQPHRYTRTFTFLTEFSQAFGDADLVVLTDIYSAGERDRLVQGQHLADCMAEYHAQVQYQPSLEEVKLFLKQTLRPGDLVLVLGARRLQLKGEEAGVQVIDSYAHHPTEMTADLEAMRAAAGDARILVVFQPHLFSRTQELGKEMGQALALADASVVLDIYPAREDPIPGITSELIIEAARAAGADVTPVHDKAEVPSVIAGMARPGDLVLTMGALAS",
    "response#2": "IAGTHGKTTTTAMVAMIYAQAGLDPTFVNGGLVKSAGTNAYLGCSRYLIAEADESDASFLHLQPMVSVVTNIEPEHMETYHGDFEEMKQTYVNFLRNLPFYGLSVMCADDPVLMELSSQVGRQVVTYGFSDEADYRIEDYQQTGFQGHYTVVAPNGERIDVLLNVPGKHNALNATAALTVAMELGYDKDIVLRSLSTFGGVARRFEVRGEAHGIRFVDDYAHHPSEIRATLAAGRLQLKSREEFISPWQRLVAVFQPHRYTRTFTFLTEFSQAFGDADLVVLTDIYSAGERDRLVQGQHLADCMAEYHAQVQYQPSLEEVKLFLKQTLRPGDLVLVLGAMGDEMR",
    "response#3": "MAVNGSFTLIDDYGHHPVEMAATIAAARGAFPGRRLVLAFQPHRYTRTRDLFEDFVKVLSTADVLLLTEVYSAGEAPIIAADSKSLARSLRVLGKVEPIFVERVDELEEAIHSIARDKDVVLVMGAMLRAGARADEVAAHLAAYRG"
  },
  {
    "reference": "MNKIIIRKTEDLKEWRRNLKCDINFIPTMGNLHDGHQKLISTAQSSNCNTNLLSIFVNPLQFDSKEDLKSYPKTVDKDIEIAFSNGADAIFIPNVTDIYPKKNKSISYLKASKELSSALCGLTRVGHFDGVCTVVYRLLKLIQPKNLFLGEKDWQQLLIIKNLIEEKKFNIKIIPVPTQRDSDGVPFSSRNKHLSKSERKSLKLFSNELENAKIIFKKDKRIDLKQLTNKLKSKNISIEYLEHLHPYSLKKVQSNDNISILAGAIKCGKTRLIDHVFLMKRKPIIAIDGPAGSGKSTITKLIAKELNLLYLDTGAMYRAISWLFKKEKIDYAKESELKKILNNISIIFKSNSISQQDVFINNFCVTEEIRSQEISSIVSKISSIKKVREFLVYEQRKIGQSGGLVAEGRDIGTTVFPNAELKIFLTASIDERAKRRKSELDLRGTEEIDFNQLRELIRKRDFEDSTRKISPLKKANDAIELLTDGYSINEVVEKIVNIYNLNIPKEIQLE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein's Proton donor must be carefully engineered to ensure that the amino acids involved in ATP + dCMP = ADP + dCDP are properly positioned for optimal catalysis.2. The ideal protein would be one that plays a critical role in pyrimidine nucleotide metabolic process, pantothenate biosynthetic process, phosphorylation through CMP kinase activity, pantoate-beta-alanine ligase activity, dCMP kinase activity, ATP binding within cytoplasm.3. A protein that can modulate the overall flux of metabolites through the Cofactor biosynthesis, (R)- pantothenate from (R)-pantoate and beta-alanine: step 1/1, (R)-pantothenate biosynthesis pathway, enhancing or suppressing its activity as needed.4. The protein should have a high affinity for ATP, (R)-pantoate.5. For general function, the protein need meet that Catalyzes the transfer of a phosphate group from ATP to either CMP or dCMP to form CDP or dCDP and ADP, respectively. The designed protein sequence is ",
    "response#1": "MPAVDGPAGAGKSTVTRLAAQALGLRYLDTGAMYRAVTWWCLENNLDLHDEPAIVEAIAHCRLHLESPDPHQPTRVWINDREVSQAIRSLEVTQRVSQIAALRGVRRLMVQQQRAIGAHGGIAAEGRDIGTHVFPEAGLKIFLTASPEERAQRRWQELQQQGHCDLSYEELLAQITARDTADQQRPYAPFRKAADAIEVCTDNLGIDEVVSKIVHLYDDASAAADVVAIDGPAGVGKSTVSRSVARELGLVYLDTGAMYRALTWLVLESGISVTDRCAIAELANRCEIQLAASLDAAQPVRVWINDREVTSAIRSLAVTANVSAIAAIPEVRQVLVKQQQQWGQKGGLVAEGRDIGTQVFPDADLKIFLTASVQERAKRRQQDFLAQGQPQVSLEQLERDIAARDWQDSNREVAPLKKAAGAVEIITDGKSITQVIAEIVLDALGDE",
    "response#2": "MILAVDGPAGAGKSTVTRLAAQALGLRYLDTGAMYRAVTWWCLENNLDLHDEPAIVEAIAHCRLHLESPDPHQPTRVWINDREVSQAIRSLEVTQRVSQIAALRGVRRLMVQQQRAIGAHGGIAAEGRDIGTHVFPEAGLKIFLTASPEERAQRRWQELQQQGHCDLSYEELLAQITARDTADQQRPYAPFRKAADAIEVCTSKLNLGEVTAIIAIDGPAGAGKSTVARLVADKLNLLYLDSGAMYRAVTWQVLQSGIDPDDEIAVAETLADCRLRLASDPGINGQVGLTRVWVNDQEVTQVIRTPEVTAKVSTVAAQPSVRQVLLKQQQDYGAKGGVVMEGRDIGTHVFPFAELKIFLTASVQERARRRQKDLAIQNQSPISLKDLEQAIDERDHKDSTRRVAPLKRAEDALTLITTEKDLTIEDVIDAVTVVLED",
    "response#3": "MVAIDGPAGAGKSTVARLVADQLGLMYLDTGAMYRAVTWRVLTTAIDPADEVAVAETLADCHIRLATEPASTPGAVGLTRVWVNEVDVTQAIRTSAVTAMVSTVAAQPAVREVLLRQQQAYGTEGGVVMEGRDIGTHVFPAAELKIFLTASVQERARRRQRDLVAQNQPPTSLADLEQAIDERDRKDSTRRVAPLQKAADAVELLTDGLTIDEVVGKIVALFDGDGGDAGTADAGTGDAGTAEAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAGTADAG"
  },
  {
    "reference": "MVFDTHLHTLFSTDSKMNIEEAIDAGEKKNLGIIITEHIDLNYPVKGEFVFDIDKYFKEYNKYRSNKVLLGVEIGMGEEIKEQNKSINDKYEFDYVLGSIHLLNGLDLYEKTIYKSSPKKEVFEMYFKTMLACLRCHEYIDSLAHIDYISRYAAYHDGEIYYNEYSDYIDEVLKFIVDREIVIEINTRRLNKKEVCENLMPIYKRYSELGGRFVTIGSDSHYKDSIGLNFKNALKMAENCNLKPVFFEKRLMKYA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. We require a protein that can specifically bind to the metabolites produced in the Amino-acid biosynthesis, L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9, L-histidine biosynthesis pathway.2. The protein must exhibit histidinol-phosphatase activity. The designed protein sequence is ",
    "response#1": "MLAYANEEAIKKTLETGYAHYYSRSRKKIWKKGETSGELQKVIEVRVDCDEDALIYVIEQEKDRACHTGERNCFS",
    "response#2": "MLAYANEEAIKKTLETGYAHYYSRSRKKIWKKGETSGELQKVIEVRVDCDEDALIYVIEQEKDRACHTGERNCFR",
    "response#3": "KPWDLPLYVEERVVDLGIIGRDVIWEQEKNVVNLISLPFGYCKMVIAGYPYVSLKGNGKEIRIATKYENITKKLLENRWGKIKIIKLNGSVELGPILNISDLIVDIVETGKTLRDNGLEVKEVLFESSACLISNVVSFAYLRKEILSFVKEVS"
  },
  {
    "reference": "MILTKAQYEEIAQCLVSVPPTRQSLRKLKQRFPSQSQATLLSIFSQEYQKHIKRTHAKHHTPEAIESYYQRYLNGVGKNGAAPVLLELANEVDYAPSLMARIILERFLQGHEQTPPSKSVINSMLRDPSQIPDGVLANQVYQCIVNDCCYGPLVDCIKHAIGYEHEVLLRDLLLKKNLSFLDEDQLRAKGYDKTPDFILQVPVAVEGHIIHWIESKASFGDECSHHAYLHGQFWSYWNRFGPGLVIYWYGFIQELDCNRERGILLKASFPTDIVTLCHSTA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be designed to localize specifically to cytoplasm, nucleus in order to perform its role in erythrocyte differentiation more effectively.2. For general function, the protein need meet that Plays a role in erythroid cell differentiation. The designed protein sequence is ",
    "response#1": "SCTACIRVDKTCSYCTDEIFKESRCDLRENLMSYGCSEANIMSVRGEMQVQQNFSIDTSLKKTQVAPQRMSMRLRAGEEVSFDMDVFQPLESPVDLYILMDFSYSMSDDLSNLKKMGQNLAEFLQSLTSNYTIGFGKFVDKVSSPQTDMRPAKLQEPWREADPPFSFKNVIRLTSNINHFSQELMKERISGNLDAPEGGFDAILQAAVCKENIGWRTDSTHLLVFSTESAFHYEADGTNVLSGILPRNDEACHLNQAGTYTYDTWQDYPSVPTLVRLLSRHNIIPIFAVTNHSYSYYEKLHKYFPISEIGVLQEDSSNILELIRVAFERIRSKLDIRADLIPKSMKTDFTSLKYEKTESGSFQVTRGEVGRFQVHVKAQEYVGGEHVCNLPEEDQQGEIHVKPSSLSDSLKIEASVICDIL",
    "response#2": "ALQTSLVFSKDFGLLVFVRKSLSIDEFRDCREEILKFLYIFLEKIGQKITPYSLDIKNTCTSVYTKDKAAKCKIPALDLLIKLLQTLRSSRLMDEFRVGELFTKFYGELALKTKIPDTVLEKIYELLGVLGEVHPSEMISNSEQLFRAFLGELKSQMTSTVREPKLPVLAGCLKGLSSLMCNFTKSMEEDPQTSREIFDFALKAIRPQIDLKRYAVPLAGLCLFTLHASQFSTCLLENYVSLFEVLSKWCGHTNIELKKAAHSALESFLKQVSFMVAKDAERHKNKLQYFMEQFYGIIRNMDSNSKDLSIAIRGYGLFAGPCKVINAKDVDFMYVELIQRCKQLFLTQTDTVDDHIYQMPSFLQSIASVLLYLDTIPEVYTPVLEHLMVVQIDSFPQYSPKMQPVCCRAIVKVFLALAEKGPVLWNCISTVVHQGLIRICSKPVVFQKGAGSESEDYHTSEEARTGKWKMPTYKDYLDLFRYLLSCDQMMDSLLADEAFLFVNSSLHSLNRLLYDEFVKSVLKIVEKLDLTLEKQNVGEQEDETEATGVWVIPTSDPAANLHPAKPKDFSAFINLVEFCREILPEKHVEFFEPWVYSFAYELILQSTRLPLISGFYKLLSVAVRNAKKMKYFEGVGPKSQKQSPEDLEKYSCFALFAKFSKEVSIKMKQYKDELLASCLTFILSLPHDIIELDVRAYVPALQMAFKLGLSYTPLAL",
    "response#3": "ALQTSLVFSKDFGLLVFVRKSLSIDEFRDCREEILKFLYIFLEKIGQKITPYSLDIKNTCTSVYTKDKAAKCKIPALDLLIKLLQTLRSSRLMDEFRVGELFTKFYGELALKTKIPDTVLEKIYELLGVLGEVHPSEMISNSEQLFRAFLGELKSQMTSTVREPKLPVLAGCLKGLSSLMCNFTKSMEEDPQTSREIFDFALKAIRPQIDLKRYAVPLAGLCLFTLHASQFSTCLLENYVSLFEVLSKWCGHTNIELKKAAHSALESFLKQVSFMVAKDAERHKNKLQYFMEQFYGIIRNMDSNSKDLSIAIRGYGLFAGPCKVINAKDVDFMYVELIQRCKQLFLTQTDTVDDHIYQMPSFLQSIASVLLYLDTIPEVYTPVLEHLMVVQIDSFPQYSPKMQPVCCRAIVKVFLALAEKGPVLWNCISTVVHQGLIRICSKPVVFQKGAGSESEDYHTSEEARTGKWKMPTYKDYLDLFRYLLSCDQMMDSLLADEAFLFVNSSLHSLNRLLYDEFVKSVLKIVEKLDLTLEKQNVGEQEDETEATGVWVIPTSDPAANLHPAKPKDFSAFINLVEFCREILPEKHVEFFEPWVYSFAYELILQSTRLPLISGFYKLLSVAVRNAKKMKYFEGVGPKSQKQSPEDLEKYSCFALFAKFSKEVSIKMKQYKDELLASCLTFILSLPHDIIELDVRAYVPALQMAFKLGLSYTPLAV"
  },
  {
    "reference": "MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARPYAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLAQVQEYRNQGIEEEIVCFGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQRYEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALAVLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAAITTELSEIVAGAAAV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex..2. The designed protein should be involved in proton motive force-driven ATP synthesis, proton motive force-driven plasma membrane ATP synthesis by localizing to plasma membrane and exhibiting ATP binding, proton-transporting ATP synthase activity, rotational mechanism. The designed protein sequence is ",
    "response#1": "GW",
    "response#2": "MYRL",
    "response#3": "GYW"
  },
  {
    "reference": "MKISYFIRRGKKTSRRSHFIKMKKNIITTQLFKPDNAFIFFSGIHGSVNRATYKYKISKTFGRFLAHISCLICILSKRIFVLSFSVIGSFCHPSIVHFDCLLFFLDTTPCL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be expressed specifically in the mitochondrial membrane2. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "MPSSFDENHEFYNEKPMAKIFRKLREEPLIPLGAGLTVFAFTQAWRPMRRGDQVSANKMFRARVAAQGFTVLAMIAGSMYYNKDREATKELF",
    "response#2": "MPSSFDENHEFYNEKPMAKIFRKLREEPLIPLGAGLTVFAFTQAWRPMRRGDQVSANKMFRARVAAQGFTVLAMIAGSMYYNKDREATKELF",
    "response#3": "MPSSFDENHEFYNEKPMAKIFRKLREEPLIPLGAGLTVFAFTQAWRPMRRGDQVSANKMFRARVAAQGFTVLAMIAGSMYYNKDREATKELF"
  },
  {
    "reference": "MIADLIQRPLLTYTLLLPRFMACFVILPVLSKQLLGGVLLRNGIVCSLALYVYPAVANQPYIEVDAFTLMLLIGKEIILGLLIGFVATIPFWALESAGFIVDNQRGAAMASLLNPGLDSQTSPTGLLLTQTLITIFFSGGAFLSLLSALFHSYVNWPVASFFPAVSEQWVDFFYNQFSQILLIAAVLAAPLLIAMFLAEFGLALISRFAPSLNVFVLAMPIKSAIASLLLVIYCMQMMSHASKAMLLVMDPISLLIPVLEK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "RPGSWGGLVLRVGQALFAAACIGVMGSSLGFASYTAFCYLIASMGLQMLWSFGLACLDGYAIRANKDLTSPILLSLFVVGDWVTAILSFAASSSAAGVVILFQKDVLFCRRYPQLPCGKYELATAFAFLSWAL",
    "response#2": "RPGSWGGLVLRVGQALFAAACIGVMGSSLGFASYTAFCYLIASMGLQMLWSFGLACLDGYAIRANKDLTSPILLSLFVVGDWVTAILSFAASSSAAGVVILFQKDVLFCRRYPQLPCGKYELATAFAFLSWAL",
    "response#3": "RPGSWGGLVLRVGQALFAAACIGVMGSSLGFASYTAFCYLIASMGLQMLWSFGLACLDGYAIRANKDLTSPILLSLFVVGDWVTAILSFAASSSAAGVVILFQKDVLFCRRYPQLPCGKYELATAFAFLSWALV"
  },
  {
    "reference": "MAAAAPVAADDDERRRRPGAALEDSRSQEGANGEAESGELSRLRAELAGALAEMETMKAVAEVSESTKAEAVAAVQRQCQEEVASLQAILKDSISSYEAQITALKQERQQQQQDCEEKERELGRLKQLLSRAYPLDSLEKQMEKAHEDSEKLREIVLPMEKEIEELKAKLLRAEELIQEIQRRPRHAPSLHGSTELLPLSRDPSPPLEPLEELSGDGGPAAEAFAHNCDDSASISSFSLGGGVGSSSSLPQSRQGLSPEQEETASLVSTGTLVPEGIYLPPPGYQLVPDTQWEQLQTEGRQLQKDLESVSRERDELQEGLRRSNEDCAKQMQVLLAQVQNSEQLLRTLQGTVSQAQERVQLQMAELVTTHKCLHHEVKRLNEENQGLRAEQLPSSAPQGSQQEQGEEESLPSSVPELQQLLCCTRQEARARLQAQEHGAERLRIEIVTLREALEEETVARASLEGQLRVQREETEVLEASLCSLRTEMERVQQEQSKAQLPDLLSEQRAKVLRLQAELETSEQVQRDFVRLSQALQVRLERIRQAETLEQVRSIMDEAPLTDVRDIKDT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Plays a role in membrane trafficking and in homotypic early endosome fusion; By interacting with SDCCAG8, localizes to centrosomes and plays a critical role in ciliogenesis.; Participates in arteriogenesis by regulating vascular endothelial growth factor receptor 2/VEGFR2 cell surface expression and endosomal trafficking2. A protein that can perform GTPase activator activity in early endosome, intracellular membrane-bounded organelle, ciliary basal body, cytosol, centrosome to support endocytosis, protein transport, cell projection organization, regulation of cilium assembly.3. Target a Polar residues, Basic and acidic residues compositional bias in the protein's composition for improved properties.4. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "GKFLEEYTSQLRRIEDALDDSIGDVWDFNLDPIALKLLPYEQSSLLELIKTENKVLNKVITVYAALCCEIKKLKYEAETKFYNGLLFYGEGATDASMVEGDCQIQMGRFISFLQELSCFVTRCYEVVMNVVHQLAALYISNKIAPKIIETTGVHFQTMYEHLGELLTVLLTLDEIIDNHITLKDHWTMYKRLLKSVHHNPSKFGIQEEKLKPFEKFLLKLEGQLLDGMIFQACIEQQFDSLNGGVSVSKNSTFAEEFAHSIRSIFANVEAKLGEPSEIDQRDKYVGICGLFVLHFQIFRTIDKKFYKSLLDICKKVPAITLTANIIWFPDNFLIQKIPAAAKLLDRKSLQAIKIHRDTFLQQKAQSLTKDVQSYYVFVSSWMMKMESILSKEQRMDKFAEDLTNRCNVFIQGFLYAYSISTIIKTTMNLYMSMQKPMTKTSVKALCRLVELLKAIEHMFYRRSMVVADSVSHITQHLQHQALHSISVAKKRVISDKKYSEQRLDVLSALVLAENTLNGPSTKQRRLIVSLALSVGTQMKTFKDEELFPLQVVMKKLDLISELRERVQTQCDS",
    "response#2": "GKFLEEYTSQLRRIEDALDDSIGDVWDFNLDPIALKLLPYEQSSLLELIKTENKVLNKVITVYAALCCEIKKLKYEAETKFYNGLLFYGEGATDASMVEGDCQIQMGRFISFLQELSCFVTRCYEVVMNVVHQLAALYISNKIAPKIIETTGVHFQTMYEHLGELLTVLLTLDEIIDNHITLKDHWTMYKRLLKSVHHNPSKFGIQEEKLKPFEKFLLKLEGQLLDGMIFQACIEQQFDSLNGGVSVSKNSTFAEEFAHSIRSIFANVEAKLGEPSEIDQRDKYVGICGLFVLHFQIFRTIDKKFYKSLLDICKKVPAITLTANIIWFPDNFLIQKIPAAAKLLDRKSLQAIKIHRDTFLQQKAQSLTKDVQSYYVFVSSWMMKMESILSKEQRMDKFAEDLTNRCNVFIQGFLYAYSISTIIKTTMNLYMSMQKPMTKTSVKALCRLVELLKAIEHMFYRRSMVVADSVSHITQHLQHQALHSISVAKKRVISDKKYSEQRLDVLSALVLAENTLNGPSTKQRRLIVSLALSVGTQMKTFKDEELFPLQVVMKKLDLISELRERVQTQCDPLKLICLPGQPSKTASHKLTIDTNKSPVSLLGLFPDFANPSDDDQVNVMGFRLLGGARVTLLASRTSQRYRIQSEQFEDLWLITNELILRLQEHFEKQGTKDFSCSFL",
    "response#3": "GKFLEEYTSQLRRIEDALDDSIGDVWDFNLDPIALKLLPYEQSSLLELIKTENKVLNKVITVYAALCCEIKKLKYEAETKFYNGLLFYGEGATDASMVEGDCQIQMGRFISFLQELSCFVTRCYEVVMNVVHQLAALYISNKIAPKIIETTGVHFQTMYEHLGELLTVLLTLDEIIDNHITLKDHWTMYKRLLKSVHHNPSKFGIQEEKLKPFEKFLLKLEGQLLDGMIFQACIEQQFDSLNGGVSVSKNSTFAEEFAHSIRSIFANVEAKLGEPSEIDQRDKYVGICGLFVLHFQIFRTIDKKFYKSLLDICKKVPAITLTANIIWFPDNFLIQKIPAAAKLLDRKSLQAIKIHRDTFLQQKAQSLTKDVQSYYVFVSSWMMKMESILSKEQRMDKFAEDLTNRCNVFIQGFLYAYSISTIIKTTMNLYMSMQKPMTKTSVKALCRLVELLKAIEHMFYRRSMVVADSVSHITQHLQHQALHSISVAKKRVISDKKYSEQRLDVLSALVLAENTLNGPSTKQRRLIVSLALSVGTQMKTFKDEELFPLQVVMKKLDLISELRERVQTQCDPLKLICLPGQPSKTASHKLTIDTNKSPVSLLGLFPDFANPSDDDQVNVMGFRLLGGARVTLLASRTSQRYRIQSEQFEDLWLITNELILRLQEHFEKQGTKDFSCSFDSVPSITVKITLQNRVVLQKVKLSIYVQPPLQLTCDQFTFDFTVPDMTSSVAFSVYLKRNYTPSELEGNAVVSYSRPTDRNPDGIPRVVQCK"
  },
  {
    "reference": "MSFFNFRKIFKLGSEKKKKQYEHVKRDLNPEEFWETIGELGDGAFGKVYKAQNKETNVLAAAKVIDTKSEEELEDYMVEIDILASCDHPNIVKLLDAFYYENNLWILIEFCAGGAVDAVMLELERPLTESQIQVVCKQTLEALNYLHDNKIIHRDLKAGNILFTLDGDIKLADFGVSAKNTRTIQRRDSFIGTPYWMAPEVVMCETSKDRPYDYKADVWSLGITLIEMAEIEPPHHELNPMRVLLKIAKSEPPTLAQPSRWSSNFKDFLKKCLEKNVDARWTTSQLLQHPFVTIDSNKPIRELIAEAKAEVTEEVEDGKEEDDDEEIENSLPIPTNKRASSDLSIASSEEDKLSQNACILESVSEKTEHNASGDKFSTKVLNEKPCPGEPENAVELVGGAVAVLPDRATELPESGREEKRPKLDRLPDTEDQEMADINSVSEGEEDHAVTSETNIEHNLKPEKERDQEKQPVLENKLVKSEDTTIQTVDLVSQETGEKEVDIHILDSEVVHAVEDTHEKLRKDDTTQKDVISDTSSVGERDEEIGAVPKTAESSAEGAQGDGGKETDEGAQILISKATEGPKASGTEEAPPVTEITETNDTDQKLVENTHEKQLPISSETTLDTSEGLGASEGREVTESGSTEEVEVEGAVSETDEEDVQSETRGAPMAVTQMDTEKNETPHEAPAQVEVQVPVPPQPSEPPPAPIPSININSEAAENKGEMGASLNTETILLPESESQKENDTDSGTGSTADNSSIDLNLSISSFLS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must have at least one UVR domain.2. Ensure the presence of a signal peptide within the protein.3. The protein must exhibit the following characteristics: Mediates apoptosis and actin stress fiber dissolution..4. The protein design should emphasize Acidic residues compositional bias.5. The protein design must be able to perform protein serine kinase activity, protein serine/threonine kinase activity in order to contribute to regulation of focal adhesion assembly, apoptotic process. The designed protein sequence is ",
    "response#1": "IMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGVPPRKASYIEDLVLMLPQHIWDNLYSRYGGGPAVNHLYICHTCQL",
    "response#2": "IMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGVPPRKASYIEDLVLMLPQHIWDNLYSRYGGGPAVNHLYICHTCQA",
    "response#3": "DFTCEKIGSGFFSEVFKVRHRASGQVMALKMNTLSSNRANMLKEVQLMNRLSHPNILRFMGVCVHQGQLHALTEYINSGNLEQLLDSNLHLPWTVRVKLAYDIAVGLSYLHFKGIFHRDLTSKNCLIKRDENGYSAVVADFGLAEKIPDVSMGSEKLAVVGSPFWMAPEVLRDEPYNEKADVFSYGIILCEIIARIQADPDYLPRTENFGLDYDAFQHMVGDCPPDFLQLTFNCCNMDPKLRPSFVEIGKTLEEIL"
  },
  {
    "reference": "MEEDEDQFLLSSLGVTSANPEDLEQKILDEATKKPDNDEGGSVEEKSTQLEGTNLLSSSQNELLNKLRAVKFEIDAVASTVENVDEIAAEKGLKKDDESDLQGLHSGSALQHALATDRLRSLKKRKIQLEKELTGLHGQSASSSADHGNLLRDLVKEKPSLKRKLKEIRKPSRRDGKKVKVVSFREDTDFDAVFDGASAGFVETERDELVRKGILTPFHKLDGFERRLQQPGPSNSRNLPEGDDENEDSSIIDRAVQSMSLAAKARPTTKLLDAEDLPKLEPPTAPFRRLRKLYKTPNSPDNEAKKRKAGKKSKKTRPLPEKKWRKRISREDSSLQGSGDGRRILTTSSCEEEELDDFDDADDNERSSVQLEGGLNIPECIFRKLFDYQRVGVQWLWELHCQRAGGIIGDEMGLGKTIQVLSFLGSLHFSKMYKPSIIICPVTLLRQWRREAQKWYPDFHVEILHDSAQDSGHGKGQGKASESDYDSESSVDSDHEPKSKNTKKWDSLLNRVLNSESGLLITTYEQLRLQGEKLLNIEWGYAVLDEGHRIRNPNSDITLVCKQLQTVHRIIMTGAPIQNKLTELWSLFDFVFPGKLGVLPVFEAEFSVPITVGGYANASPLQVSTAYRCAVVLRDLIMPYLLRRMKADVNAHLTKKTEHVLFCSLTVEQRSTYRAFLASSEVEQIFDGNRNSLYGIDVMRKICNHPDLLEREHSHQNPDYGNPERSGKMKVVAEVLKVWKQQGHRVLLFSQTQQMLDILESFLVANEY",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should contain one or more Helicase C-terminal domains that are essential for its function.2. The protein design must feature the Nuclear localization signal, DEGH box motif.3. Include a signal peptide as an essential component of the protein.4. For general function, the protein need meet that Essential factor involved in transcription-coupled nucleotide excision repair (TCR) which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes; It is required for transcription-coupled repair complex formation.5. A protein that contributes to response to gamma radiation, transcription-coupled nucleotide-excision repair, DNA repair, cellular response to DNA damage stimulus by functioning in nucleus with DNA binding, ATP-dependent chromatin remodeler activity.6. Prioritize Basic residues, Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "MAAVPQNNLQEQLELHSARKLHNRLNLSKPKSSGFTFKKKASSGNSASVTSVSAARTPVLSDKDVNVTEGSSFSEPQACPAGPPARINDFFKNAPAGLQSRGAGSTPLLPDVSQTPQEASCTPRLAPAAKTPRDTVFKKLDFSSSLDSLGALNGWDDMDDFDTSGTPKAFAASPRKPVARVSTAQKSKRAKRNFCKAQLKKADAAEADLTPASAASKPVELAGAGKGDCDGVGGGVICIDDDDGPVSQVLILDSQESHSLETHLGGERDHPEDKKVLDTVELHSAEEVPCIELEDDDYDIDFVPPSPEEEVSSATSSSLKCSSMLKDLDTPHREKDVLSPAQDLLSKPEKLTPQEPDPETTAGCEARPLSASLEGSVYTETVGRSLVTATSTSKGRKLEDNEIVNFTFSSVAKWKVPNIVRFSTKRCGEIGRLPMEWSNWAVSLLRSGKVKMLGRCVAAPPFLTMMQEIMLYVSFYTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACISPGVTIVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEAASLSVWRCWPDSLGSPVDDTVSASAPDAFPKGESCPAGSSVQELNFPHLPSHCGTTRECLLATTPGKTGFSATPQNPFERPLFSPHLQKSFVSSNWAETPRIEKRNESSYFPGNVLTSTAVKDQNKRAAPIDDLERETQAACDIDNFDIDGFDDDDDDDWENIIHDLAASKSSTAASQPIKEGRPVKPVSERISSAKANCLPVASTAQDKNSESVQNYTDKSAQNLASRNLKHERFQS",
    "response#2": "MAAVPQNNLQEQLELHSARKLHNRLNLSKPKSSGFTFKKKASSGNSASVTSVSAARTPVLSDKDVNVTEGSSFSEPQACPAGPPARINDFFKNAPAGLQSRGAGSTPLLPDVSQTPQEASCTPRLAPAAKTPRDTVFKKLDFSSSLDSLGALNGWDDMDDFDTSGTPKAFAASPRKPVARVSTAQKSKRAKRNFCKAQLKKADAAEADLTPASAASKPVELAGAGKGDCDGVGGGVICIDDDDGPVSQVLILDSQESHSLETHLGGERDHPEDKKVLDTVELHSAEEVPCIELEDDDYDIDFVPPSPEEEVSSATSSSLKCSSMLKDLDTPHREKDVLSPAQDLLSKPEKLTPQEPDPETTAGCEARPPFSRLCLLEFKHKNEEKAQQLSLKASQTLSSCLEKGVTLSNFKAPIEKIASSYRYLILLRSKNPLSLIKSVHAFLKSAPSIPCSVNMDVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLDSHLSPFSRLCLLEFKHKNEEKAQQLSLKASQTLSSCLEKGVTLSNFKAPIEKIASSYRYLILLRSKNPLSLIKSVHAFLKSAPSIPCSVNMDTHLSFSASDSSGSASRMGGKSWVLDSVFGPGHATREVYAAVAGNLIEQLREGFNATIFAYGQTGSGKTHTMTGPGNGGGGGAEGSSKGGGGSGAEGIIPLAVHDVFRLAEGDSDRHYDLTVTFMEVYYEELIDLLAASGGKEGTKGRQALKVRENADGFFVDGLQSFPVTSAAQALQLLVAGEKRRHTGETKMNEKSSRSHTIFSLLLKSRRKGVELGRTDGATRCATLTLVDLAGSERLDKTAAEGQRAKEGAAINKSLLTLGTVINKLSEGAATSPYRDSNLTRILQHSLGGNAKTAVICAVAPEVVHRDESTSTLGFATRAKR",
    "response#3": "MAAVPQNNLQEQLELHSARKLHNRLNLSKPKSSGFTFKKKASSGNSASVTSVSAARTPVLSDKDVNVTEGSSFSEPQACPAGPPARINDFFKNAPAGLQSRGAGSTPLLPDVSQTPQEASCTPRLAPAAKTPRDTVFKKLDFSSSLDSLGALNGWDDMDDFDTSGTPKAFAASPRKPVARVSTAQKSKRAKRNFCKAQLKKADAAEADLTPASAASKPVELAGAGKGDCDGVGGGVICIDDDDGPVSQVLILDSQESHSLETHLGGERDHPEDKKVLDTVELHSAEEVPCIELEDDDYDIDFVPPSPEEEVSSATSSSLKCSSMLKDLDTPHREKDVLSPAQDLLSKPEKLTPQEPDPETTAGCEARPPFSRLCLLEFKHKNEEKAQQLSLKASQTLSSCLEKGVTLSNFKAPIEKIASSYRYLILLRSKNPLSLIKSVHAFLKSAPSIPCSVNMDRLPSLAFVKGQCVLISRARFEADISYSEDLIALFKQMDSRKYDVKTRKWSFLLEEYSKLMERVRGLPQVQLDPLLV"
  },
  {
    "reference": "MQLKDLLYKIQLTSVSGSTSVEISDVQTDSRAVTPGCCFIALRGTATDGHQYIAQAVAQGAIAIVAEELPATMAPHVTYIQVKDTALACGWISHTFYSEPSTQLRITGVTGTNGKTTVATLLYKLFSALGFNCGLISTVENRMGNEILPATHTTPDAVSLNRLLANMVEAGCTHVFMECSSHAIHQQRIAGLQFAGAIFTNITHDHLDYHKTFDEYIRVKKSWFDALPATAFSLTNADDKRGNVMLQNTRARKQTYALKTLATYKGKILDSNLTGLQMMVDDVEVYFKMIGEFNAYNLMAVYGAAVSLGTDKHEALLKLSMLTGAEGRFECVQTNHRTVMGIVDYAHTPDALLNVLATINKIRQGAGQLITVVGCGGNRDTTKRPVMAQVACEHSNKVIFTSDNPRNEDPLKIIQDMESGVSITQKKKFISIPDRKEAIKTAVLMSSADDIILVAGKGHEKYQEVQGVKHPFDDKVVLMDMLNMMDK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The presence of Mur ligase C-terminal domains is critical for the interaction of the designed protein with its intended targets.2. The ATP binding site should be stable and able to withstand changes in environmental conditions.3. The designed protein should be optimized for UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity activity in order to improve cell wall organization.4. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..5. The protein design must feature the Meso-diaminopimelate recognition motif motif. The designed protein sequence is ",
    "response#1": "MKEISGLTADSRQVRPGDLFVAVAGTKANGTAYIADAVSRGAAAIVAADAHPDAGAPVFMLPDPRRFLAKAAAAFYGAQPAMMVAVTGTNGKTSVAVFVRQIWQKAGFRAASLGTIGLIAPDGSVRPSLTTPDPVSLHQMLADLADDNVTHLSMEASSHGLQQRRLDGVRLSAGAFTNLTRDHLDYHRTLEDYLRAKLRLFDTLLEEGSHIVVDADEPQAETIRMIAKKRGEIFGSIGRKGETLKLVSVTRMSDGVRLVVEAEGERHYVPLPLVGDFQVSNALVAAALALATAGATPQQIFAALPQLSGVRGRMQRVPSAEGCFIVDYAHTPDALSQVLASLKTHCKGQLWAVFGCGGDRDAGKRPLMAQAGLAGADQVILTADNPRTEDPNAILQDMQAGMSAEQYQRTHIEPARQTAIEYAVSQASADDIIVIAGKGHEDYQEINGERHHFDDREHAEAALQKRGLI",
    "response#2": "MSGRLEPVRAGQSFAVLVDYAHTDDALKNVLATLRPLCKGKLIALFGCGGDRDKTKRPRMARVCWEMADRIFVTSDNPRTEDPIKIIEDILPGFENPNDPRITVQPDRRAAIEAAIQSAGPEDIVLLAGKRS",
    "response#3": "MTSAPRPPSLTELADLLPLAVGEPPTGVALDSRQVRPGDLYVGLPGATTHGARFAAQAQASGALALLTDAAGTALAADTSLPVLTVDDPRAAMAALAADVYGRPSADLTVVGVTGTNGKTTTTFLIEQGLRGVGERAARLGTLGFEMEGHVFGDTLTTPEADDIARCLLEVQQRGGRYLVMEVSSIAVVQERVSGLRFRAAAFTNLTQDHLDFHGSLEAYAEAKAALFTRLAPELSVLHVGDAFGRELSRRVHSGRLTVARQEADVRVAEASLGASGIRAKLITPQGDCSIDSALVGEHNLENLLVALGVLVEAGLEPRAAADGIAAVPGVPGRLELIGSHPSGAQVFVDYAHTPEALAKALQALRAHAEGRLVVIFGAGGDRDTGKRPLMGKAAAGNADHVIVTDDNPRSEDPAAIRKAVLAAAPGAEEIGDRREAIRHAVAGLNKGDVLLVAGKGHESGQEVAGVVHPFDDREVVREALARRAGRQPQGGGEV"
  },
  {
    "reference": "MQQQQAWAAGLGGLAVAGVGEEGGGGGPAPTTVDEASMERSKSFVKALQELKNLRPQLYSASEYCEKSYLHSEQKQMVLDNLKDYAVRALVNAVDHLGTVAYKLTDLYEQQASEVSTLELKVACLNQQVLTCQTYTDKEGIRQQQMTGTATRHHKHYIVPTLANKRMQAFSEMQTDADIDSRPRPYPSAKTLFWHLASEKNSKTNGARQSEFVLEETKATKPASRGKEPSTSPLPKHLQTNLASSDFAMHNVGMKDQPGVRHLSSFSSFDNPRGRQIQKAPLRTKSMLAAFFVKHKSGKMKNVSVR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Part of a WAVE complex that activates the Arp2/3 complex . The designed protein sequence is ",
    "response#1": "LRFL",
    "response#2": "GEREELLQQIRSKTFNLRRM",
    "response#3": "MF"
  },
  {
    "reference": "MRRAVGFPALCLLLNLHAAGCFSRNNDHFLAIRQKKSWKPMFIYDHSQDIKKSLDIAQEAYKHNYPAPSEVQISKRHQIVDSAFPRPAYDPSLNLLAASGQDLEIENLPIPAANVIVVTLQMDIDKLNITLLRIFRQGVAAALGLLPQQVHINRLIEKKSQIELFVSPGNRKPGEPQALQAEEVLRSLNVDVLRQSLPQFGSIDVSPEKNVLQGQHEADKIWSKEGFYAVVIFLSIFIIIVTCLMIIYRLKERLQLSFRQDKEKNQEIHLSPIALQQAQSEAKAAHSMVQPDQAPKVLNVVVDPQGQCTPEIRNTASTSVCPSPFRMKPIGLQERRGSNVSLTLDMSSLGNVEPFVAVSTPREKVAMEYLQSASRVLTSPQLRDVVASSHLLQSEFMEIPMNFVDPKEIDIPRHGTKNRYKTILPNPLSRVCLRPKNITDPLSTYINANYIRGYSGKEKAFIATQGPMINTVNDFWQMVWQEDSPVIVMITKLKEKNEKCVLYWPEKRGIYGKVEVLVIGVNECDNYTIRNLVLKRGSHTQHVKHYWYTSWPDHKTPDSAQPLLQLMLDVEEDRLASEGRGPVVVHCSAGIGRTGCFIATSIGCQQLKEEGVVDALSIVCQLRVDRGGMVQTSEQYEFVHHALCLFESRLSPETVQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a binding site that is specific to the target substrate ligand.2. For general function, the protein need meet that Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form3. The protein should be optimized for stability and solubility when expressed in cytosol, cell junction where it will be involved in neuron differentiation, peptidyl-tyrosine dephosphorylation.4. The designed protein must have at least one Tyrosine-protein phosphatase domain.5. The designed protein should contain Extracellular topological domains.6. Incorporate a signal peptide in the protein design.7. The protein's Phosphocysteine intermediate must be carefully engineered to ensure that the amino acids involved in H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] +   phosphate are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "NDGLIHIYNYRKHDYEKYSPSLLCEEICTAMCRHMEILPAAVLLFGIREHKNGKTGAGLTPRTLEANHNWQLPGEQLNCIVRYCLRMRFRVPELDRQLSAIDAQCQNYLYHQMRYDMLHEQINEIRYPEHKDKVMGLAVMDMIIDKDEKQLQAEAIEKAYKSYLPHTLWRAHRIFVRPKIRRSFRQMSRDQFNCDTLKWHYVHQICHLAPTYLTEQFKATVEYLPNESATEPLSLPHGKSTGSIGGSSARTQSTSPLISGSNTTLFTTMTGNHSQCPGQQQPQQPDQLHQQQQQQHPHQTQSSNRNRPIPQQPNTIPVYVRLCFHDSPEPGLKVARVTSEATLKWSLVAIVEDIFSLYKESDKLVRLESVGFPKGYHMRFETEHEMKSFISYLSIYTRLTVKWS",
    "response#2": "DLGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPYIVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKELLKHPFWYHGHLSGRDAEKLLTEKGKSGSFLVRESQSKPGDFVLSVLTNEEKHENVDRKTKVTHVMIRYQDGKYDVGGGERFDTLADLVEHYKKNPMVEKSGIVVHLKQPGTI",
    "response#3": "MHVNTNANFYKDSGITYLGIKANDTQEFNLSAYFERAADFIDQALAQKNGKVLVHCREGYSRSPTLVIAYLMMRQKMDVKSALSIVRQNREIGPNDGFLAQLCQLNDLFLAEFQSIPRVFSKFPIKEARKSFNQNKNRYVDILPYDYNRVELSEINGDAGSNYINASYIDGFKEPRKYIAAQGPRDETVDDFWRMIWEQKATVIVMVTRCEEGNRNEVSLAEALEVRGGPLQEEEIWAVLNQSAESLQEVFRRVSIADPAALGFIISPWSLLLLPSGSVSFTDENVCSQDLRAFTAPEVLQSHSLTSLSDVEKVHIYSLGMTLYWGADHEVPQSQPIKLGDHLNSILLGMCEDVLYARVSVRTVLDACSAHIRNSNCAPSFSYVKQLVKLVLGNISGTDPLSHSSAPTLVNVTSEAPTQLHVSWVHAAGDRSSYQVTLYQESTRTATSIVGPKADSTSFWGLTPGTKYKVYFPSESCNEVIPRIYVGNATVAQDISRLKHLGITHVLNAAEGKSFMHVNTTAQFYEGTNITYLGIKANDTEEFNLSIFFEKAAEFIDKALTRKDAKVLVHCREGYSRSPTLVIAYLMLRQKMDVRTALSLVRQNREIGPNDGFLTQLCQLNEKLINQNKNRYVDILPYDYNRVELSDINGDAGSNYINASYIDGFKEPRKYIAAQGPRDETVDDFWRMIWEQKATVIVMVTRCEEGNRNKCAEYWPSMETSCNEVIPRIYVGNATVAQDISRLKHLGITHVLNAAEGKSFMHVNTTAQFYEGTNITYLGIKANDTEEFNLSIFFEKAAEFIDKALTRKDAKVLVHCREGYSRSPTLVIAYLMLRQKMDVRTALSLVRQNREIGPNDGFLTQLCQLNE"
  },
  {
    "reference": "DGDCLLVEALDRQGKVVAMLSLVPWGTTGVSLDLMRRSPQSPNGTIELMVSELALGGEQLGIIRISLNFAMFRSAFEQGAQLGAGPVARLWRGLLLFFSRWWQLETLYRSNMKYQPEWVPRYACYEDARLIPRVGVASVIAEGFLVLPFSRRDKAHTGHHPAVPARLVESGLLHADGSAPDVSGLRRVEADGQRSHGRLPEQVRVRLSKLKALQRSGVDAYPVGAPPSHTIAAALEADDRETVSVSGRILRIRDYGGVLFAQLRDWSGELQVLLESSRLDGDAPDFTAVDLGDLVEMTGHMGFSKNGTRSLIVRDWRMLGKCLRPLPNKWNGLTDPEARVRTRYVDLAVNPESRELIKARSSVVRSVRDTLFAKGFIEVETPILQQVHGGATARPFVTHINTYDMDLFLRIAPELYLKRLCVGGMERVFELGRAFRNEGVDFSHNPEFTLLEAYQAHADYTVWIDGCRELIQNAAAAAHGEQIVMRPRGGAHGADLEPVDISGMWPVKSVHDAVSEALGERVDPDTSLAALRRLCDAAHIAYRPHWDAGAVVLELYEHLVEDRTERPTFYTDFPTSVSPLTRPHRSRAGVAERWDLVAWGVELGTAYSELTDPVEQRRRLQEQSLLAAGGDPEAMQLDEDFLQAMEYAMPPTGGMGMGIDRVVMLITGRSIRETLPFPLAKPH",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that LPG synthesis contributes to the resistance to cationic antimicrobial peptides (CAMPs) and likely protects M.tuberculosis against the CAMPs produced by competiting microorganisms (bacteriocins); Catalyzes the production of L-lysyl-tRNA(Lys)transfer and the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge; In fact, the modification of anionic phosphatidylglycerol with positively charged L-lysine results in repulsion of the peptides.2. The designed protein should be optimized for magnesium ion binding, nucleic acid binding, ATP binding, lysyltransferase activity activity in order to improve lysyl-tRNA aminoacylation, lipid metabolic process, response to antibiotic.3. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.4. The desired protein should be able to perform ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-   tRNA(Lys)5. The protein should be able to bind Mg(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MIQEQEADSLGYFATRRDKGVIFAKSDKAAVAYRNVMDSCLAAGDPIGPKDHWDAAIRAFVEHANQKGLAPAVVGTCAEAARAYARAGFKVRALGDESVIHTKNFNLNDSKLIDVRHAHQKLIEQGYSARIRRHKDISNSEMQRLISFADRWRQHGDERGFSMALGRLGDPLDGDCVFVEALDNEGETRGLLSFVPWGNAGLSLDVMRRDLENAGSGVTEFMVASLLLQAREVGVQRVSLNFAFLRETIEAGEDVGATMTQRINRAVVGFMSQKFQIEQLYRSNMKYQPTWQTRYLSQI",
    "response#2": "MKKSLLQDFGGQDSLSYFATRREKLLIFSTDRQAAVSYRIINSVCLASGDPVGNPGSWADAIEQWKENARTYGWVPAALSVSEDGARAYANAGLSVVSMGDEAVLYTDRFSLNNTSLTEVRHAVNRVRKGGYSVTMSYHGDLSAEELSSLVANADSWRHGETERGFSMALGRLGDPADARSILVAAHDASGQMVGLLSFVPWGKTGISLDVMRRSPDAPNGIVEFMVAELMEGAPEFGVSKVSLNFAMFRSIFENAEHFGASPTTRLASRTLGVFDRFLQLERLYKFNQKFAPEWLPRYLRTLLAEHGERDSLGYFATRRDRAAIFSPSGKAAVSYRVVAGVSLAGGDPIGDLEAWQPAICAFLDQAKAYGWTPAAVGASEEGARAYHRSGLHVLELGDEAVIDIAAFSLGGRGMRAVRQAVNRVERAGYTLRIRRHSALPPDEMHRVIERARAWRDTETERGFSMALGRLGDPADGRCVLVEALDAQGGEAAVLSFVPWGEHGLSLDLMRRDRDADNGLMEFMVAGLAGHAPALGIERISLNFAVFRAVFEEGARMGAGPVLRAWRRTLLFCSRWWQLESLYRSSAKYQPIWVPRYLAGVDPPLPAAWFAQRALLKAYGEQDSLGYFATRRDKAVVFSPSGKAAVTYRVEAGVCLASGDPVGDREAWPHAIAAWLDLARRHAWAPAVMGASEAGAKAFARAGLGALQLGDEAIVHVPGFDLDGRDMRVTRQAVHRVRRTGATTRIRRHATLTAEEMEEVVGKADAWRDTETERGFSMALDRLGDPADGDCLLVEALSADGSLLALLSFVPWGRDGVSLDVMRRDRQTAPNGVMEFMVADLCAAAPKLGVRRISLNFAVFRSVFEEGARIGAGPVLRLWRRLLLFFSRWWQLEALYRSNAKYHPEWYPRFL",
    "response#3": "MIRLSQVPHISGLILGVFSVLVFLWSLSPTLRHIVRVPREYIDSYYFDAPDTSLSWALVVGLLAAALASRKRIAWWILTIYLFLSALNNVNTTIREKNINSAVAIVVLLIAVGILIAARPEFYTRVRRGAGWKALGVLIVGLALATLVGWGLVELFPGTLEGTAQRFFWALNRVTAAGAVDNENFEGHPRAFVNTILGLLGAIALLAAVITLFRQESRRAMMVEQEADSLGYFATRRDKGVIFAKSDKAAVAYRNVMDSCLAAGDPIGPKDHWDAAIRAFVEHANQKGLAPAVVGTCAEAARAYARAGFKVRALGDESVIHTKNFNLNDSKLIDVRHAHQKLIEQGYSARIRRHKDISNSEMQRLISFADRWRQHGDERGFSMALGRLGDPLDGDCVFVEALDNEGETRGLLSFVPWGNAGLSLDVMRRDLENAGSGVTEFMVASLLLQAREVGVQRVSLNFAFLRETIEAGEDVGATMTQRINRAVVGFMSQKFQIEQLYRSNMKYQPTWQTRYLGWVPAAAGWIVGVIATLSLLASISPLIRAVIKVPREFVDDYIFNFPDTSFAWAFVLALLAAALAARKRLAWWILLGYMVASVGWNVAGAADRGERWFQEAGEMAGLAFHLAAIVCLVLARHQFWARVRRGALLKAALTLLAGMTVGTLVGWALLEVFPGSLARSDRFWYALNRVGAFAGADADTFAGHPHVFVNALLGLFGALALMVAAIVLAM"
  },
  {
    "reference": "MSQEYTEDKDVTLTKLSSGRRLLEALLILIALFAVWLMAALLSFNPSDPSWSQTAWHEPIHNLGGAPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQSTDDYIDYFAVSLRLIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHSSGGTIMLLCIWAAGLTLFTGWSWVSIAEKLGGWLLNILTFASNRTRRDDTWVDDEEYDDEYDEETDGVQRESRRARILRGALARRKRLAEKFSNPRGRQTDAALFSGKRMDDDEDIQYSARGVAADPDDVLFSGNRATQSEYDEYDPLLNGHSVTEPVAAAAAATAVTQTWAASADPIMQTPPMPGAEPVVAQPTVEWQPVPGPQTGEPVIAPAPEGYQPHPQYAQPQEAQSAPWQQPVPVASAPQYAATPATAAEYDSLAPQETQPQWQAPDAEQHWQPEPIAAEPSHMPPPVIEQPVATEPEPGIEETRPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKENVPVKPTVSVAPSIPPVEAVAAAASLDTGIKSGALAAGAAAAAPAFSLATGGAPRPQVKEGIGPQLPRPNRVRVPTRRELASYGIKLPSQRIAEEKAREAERNQYETGAQLTDEEIDAMHQDELARQFAQSQQHRYGETYQHDTQQAEDDDTAAEAELARQFAASQQQRYSGEQPAGAQPFSLDDLDFSPMKVLVDEGPHEPLFTPGVMPESTPVQQPVAPQPQYQQPVAPQPQYQQPQQPVASQPQYQQPQQPVAPQPQY",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be optimized for ATP binding, DNA binding activity in order to improve cell division.2. For general function, the protein need meet that The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region; FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif .; Essential cell division protein that coordinates cell division and chromosome segregation3. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "MKPIAAFQEYLEVERQYSPETVTAYLSDLQEFQAFLKANGGFTDFRHVDDLDVQTYLTDLNKQDLARTSIARKISSLRSFYRYLAR",
    "response#2": "KMPHLLIAGATGSGKSVCINTLIISLIYKYSPEDVKLLMVDPKVVELNIYNDIPHLLIPVVTEPKKAAGALYWAVNEMTRRYKLFAETNVRNIESYNELLKKGKGVEKLPLIVIVIDELADLMMVCPNDIEDYIGRLAQMARAAGMHLVIATQRPSVDVITGVIKANIPSRISFAVSSQIR",
    "response#3": "RMPHLLVAGTTGSGKSVAVNAMVLSLLFKASHNDLRMLMIDPKMLELSVYQGIPHLLAPVVTDMKEAANGLRWCVAEMERRYKLMSAVGVRNLAGFNKKVKDAEEAGQPLMDPLFKPNPDLSEVPRPLQKLPFIVIFIDEFADMMMIVGKKVEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRIAFQVSR"
  },
  {
    "reference": "MRVRGILRNYPQWWIWGILGFWMIMLCNVMGNLWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQEVFLENVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLKCSTYNGTYTDNYQEKIKNCSFNTTTEIRDKKQSGYALFYKFDVVPLNGNNTNSSEYILINCNTSAITQACPKVSFDPIPIHYCTPAGFAILKCNNGTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEDTIIRSENLTDNVKTIIVHLNESIEINCTRPNNNTRKSTRIGPGQTFYAAGEIIGKIRLAYCNISEEVWKKTLQRVGRKLKEHFPNKTIQFQPPSGGDLEVTTHSFNCRGEFFYCDTSELFNSTYMSNGTEGNISPSTIIMLPCRIKQIINMWQGVGRAMYAPPIKGSITCRSNITGLLLTRDGGLNRSTEEPEIFRPGGGDMRNNWRSELYKYKVVGIKPLGVAPTGAKRRVVEREKRAVGIGAVFLGSLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNRSEADIWDSMTWMQWDKEISNYTGTIYRLLEESQTQQEKNEKDLLALDSWQNLWNWFSITNWLWYIKIFIMIVGGLIGLRIVFAVLSIVNRVRQGYSPLSFQTLTQNPGGPDRLGRIEEEGGEQDKDRSIRLVNGFLPLVWDDLRNPCLFSYHRLRDFILVI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must be able to regulate fusion of virus membrane with host plasma membrane. The designed protein sequence is ",
    "response#1": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKIICTTAVPWNSTWSNKSYEDIWNNMTWVEWEREISNYTNQIYDILTESQTQQDKNEKDLLELDKWASLWNWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSLQTPS",
    "response#2": "DVTNGLGTYYVLDRVYLNTTLFLNGYYPTSGSTYRNMALKGSVLLSRLWFKPPFLSDFINGIFAKVKNTKVIKDRVMYSEFPAITIGSTFVNTSYSVVVQPRTINSTQDGDNKLQGLLEVSVCQYNMCEYPQTICHPNLGNHRKELWHLDTGVVSCLYKRNFTYDVNADYLYFHFYQEGGTFYAYFTDTGVVTKFLFNVYLGMALSHYYVMPLTCNSKLTLEYWVTPLTSRQYLLAFNQDGIIFNAEDCMSDFMSEIKCKTQSIAPPTGVYELNGYTVQPIADVYRRKPNLPNPSPLNWERKTFSNCNFNMSSLMSFIQADSFTCNNIDAAKIYGMCFSSITIDKFAIPNGRKVDLQLGNLGYLQSFNYRIDTTATSCQLYYNLPAANVSVSRFNPSTWNKRFGFIEDSVFKPRPAGVLTNHDVVYAQHCFKAPKNFCPCKN",
    "response#3": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKIICTTNVPWNSTWSNKSYEEIWNNMTWIEWEKEINNYTSIIYDLITESQNQQERNEKDLLELDKWASLWNWFDITRWLWYIKIFIMIVGGLIGLRIVFAVLSIVNRVRQGYSPLSFQTPVD"
  },
  {
    "reference": "MSSLPQLVSATANPHKLAEIAGILDGLVELLPRPAHVAEVVEDAGTLEGNARLKAAAICAATGLPAVSDDTGLFVDALGGEPGVDTAYYAGPHATYAENRAKLLAVLSGVADRRASFRTIVMVVWPDGRELCVEGMCSGTIAPEERGERGFGFDPLFVPDDGDGRTFSLMTDSEKNEISHRGRAFVALVDALREQAAFTPPA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP2. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein should have nucleotide binding, dITP diphosphatase activity to facilitate purine nucleoside triphosphate catabolic process.4. The protein requires Mg(2+) to activate a critical catalytic site.5. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "MKILGVDPGSRITGYGVIDCVRRQMRYVASGCIRTSSDDLATRLSLIFSGLSEVIEQFGPDEMAVERVFVNVNPQSTLLLGQARGAAICALVSGDLPVAEYTALQIKQAVV",
    "response#2": "MPETLVLASNNAGKVKEFNALLAPLGITVRPQADFAVPEPEETGTTFAENALLKARAAADASGLPALADDSGLAVDALGGAPGIYSARYAGEPKSDARNNAKLLHELAGVPEAQRGARFVCALALVRHANDPQPLIAEGTWEGRILEAPRGTNGFGYDPYFYLPDLGLSAAQLSPEQKNAISHRGQALAQLVQKLETNG",
    "response#3": "MRIILLGAPGAGKGTQAKFITDKFGIPQISTGDMLRAAVKQGTELGKEAKETMNAGKLVSDDLIIGLVKERISQDDCKNGQG"
  },
  {
    "reference": "MKVVKAYEMAELDRITIEEIGIPSPVLMENAARGVVTALLERFPGVKRILVVAGKGNNGGDGIAVARMLQLKGIQADLYLPMGEPKGDGALQLDIFKRLGYKPLVKKPNYKNYELVVDALFGTGFEPPLRGDISKTVAEINSSGVPVVSVDIPSGLSADTGKVFEPCIKAALTVTFQFPKLCHILYPSAKLCGEVVVWDISIPEKFATKIYRELITPESLKLPRREVDTYKNREGHVLIVGGSAGKTGAVIMSALAATRTGSGLVTVGVPKGLNLIFESRLVEEMSLPLQGEDRLSIFGVDTILKIQDKFSALALGMGMGRYEEGQDIVIELLEKWEKPILLDADGINNLADSGELEILKKRKGVTILTPHIGEFSRLTGLSSEEIIHHQTDVAQEFSKRYRCYLVLKGARTVVSNPEGMTFISTRGTPAMAKGGVGDVLSGMLVSLIGKSLEPTEALRLGVYLHGVAGELAEERLHTESLRARDIIEEIPRAYKSIENFLKGADTINQDDRQG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must be able to metal ion binding. The designed protein sequence is ",
    "response#1": "LPDAYLSVAEALRHGGYDCGARVDIDWIASDDAEGLLAEGRLHELDGIVVPGGFGFRGIEGKIAAVGYARENDIPFLGLCLGMQCAVVEFARNVCDLAGANSSEFDPHTPHPVIDLMDEQRDVIDMGGTMRLGIYPARLEPGSIVSAAYGEEVIYERHRHRYEVSNKHRPRLEEMGLVCSGTSPDGRLVEFVELPTDKHPFFVATQAHPEFKSRPDRPHPLFAAFVE",
    "response#2": "VRIGLVGKYVNLPDAYLSVAEALRHGGYDCGARVDIDWIASDDAEGLLAEGRLHELDGIVVPGGFGFRGIEGKIAAVGYARENDIPFLGLCLGMQCAVVEFARNVCDLAGANSSEFDPHTPHPVIDLMDEQRDVIDMGGTMRLGIYPARLEPGSIVSAAYGEEVIYERHRHRYEVSNKHRPRLEEMGLVCSGTSPDGRLVEFVELPTDKHPFFVATQAHPEFKSRPDRPHPLFAAFVRR",
    "response#3": "VRIGLVGKYVNLPDAYLSVAEALRHGGYDCGARVDIDWIASDDAEGLLAEGRLHELDGIVVPGGFGFRGIEGKIAAVGYARENDIPFLGLCLGMQCAVVEFARNVCDLAGANSSEFDPHTPHPVIDLMDEQRDVIDMGGTMRLGIYPARLEPGSIVSAAYGEEVIYERHRHRYEVSNKHRPRLEEMGLVCSGTSPDGRLVEFVELPTDKHPFFVATQAHPEFKSRPDRPHPLFAAFVRIGLVGKYVNLPDAYLSVAEALRHGGYDCGARVDIDWIASDDAEGLLAEGRLHELDGIVVPGGFGFRGIEGKIAAVGYARENDIPFLGLCLGMQCAVVEFARNVCDLAGANSSEFDPHTPHPVIDLMDEQRDVIDMGGTMRLGIYPARLEPGSIVSAAYGEEVIYERHRHRYEVSNKHRPRLEEMGLVCSGTSPDGRLVEFVELPTDKHPFFVATQAHPEFKSRPDRPHPLFAAFVARE"
  },
  {
    "reference": "MIIGIGSDLIDIRRVEKSIERFGTRFTERCFTDIERAKSEGRKNKAASYAKRFAAKEACSKALGTGLAQGVFWRDMGVVNLPSGKPTMQLTGGAARRLAAMLPENHRAAIHLTITDDFPLAQAFVIIEALPIAG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be involved in fatty acid biosynthetic process by localizing to cytoplasm and exhibiting holo-[acyl-carrier-protein] synthase activity.2. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.3. The protein should be designed to have high stability and activity towards apo-[ACP] + CoA = adenosine 3',5'-bisphosphate + H(+) + holo-   [ACP]. The designed protein sequence is ",
    "response#1": "ILGMGTDIVEIARVQAIIARSGDKLAHRILTPLELSEYQQHQQPVRFIAKRFAAKEAAAKALGTGFRNGLELRHFQVIHDELGKPHLLVSDKANEMAVRLSVKSWHLSISDERHYAIALVIVK",
    "response#2": "MGTDIVEIARVQAIIARSGDKLAHRILTPLELSEYQQHQQPVRFIAKRFAAKEAAAKALGTGFRNGLELRHFQVIHDELGKPHLLVSDKANEMAVRLSVKSWHLSISDERHYAIAG",
    "response#3": "VFSGMGSQWPGMGIELLKFPVIAKAVQKCDDVLQPRGIDIYDILTNKDKSTFNNILNSFVGIAAMQIGLVDLLTSVGIVPDNIIGHSVGELGCAYADGCLTAEQMILSAYSRGLASIETKVIYGSMAAVGMGYEDIKHMCPPDIEVACHNASDSSTISGPAESMKEFVAQLQAKKIFAKEVLCSNIPYHSRYIAPAGPKLLAYMNEVIPQPKPRSRKWVSTSVPRSKWSTASAKLSSAEYHTNNLLSPVLFEIVISGIAGRFPESRNVETLKKNLLNKTDLITDDDRRWKLDHPDIPQRTGKVHDVGKFDALFFGVHFKQAHTMDPMCRMLLEHAYEALIDAGVNPKQVRGTRTGVFMGACFSESEKTWFYEKLQVNGFGITGCSRAMMANRISYWLGVTGPSYTLDSACSSSLYAVEHAYRCIQDGQCDAAIVGGANLCLHPYVSLQFSRLGVLSPDGRSKVFDIDADGYARSEAIVVLYLQKAKEAKRVYATVVYGKTNCDGFKEQGITFPSSLMQSELLRECYEDCGVPPNILSYLECHGTGTKVGDPEEVNAIDKICTKGRSSPLLIGSVKSNLGHSEPASGVCQIAKVIIAMETGIIPPNLHYKRPREGVKALEEGRLKVISEPTPWEGGYVGINSFGFGGANCHILLKSPYKKKDSRPLVIQLGRTRFSVPVTASGDEWVAAEVLREEFVHSEKDNDAVDTTSELKDDTEATIELFARFLAFAADKLNARNECTSACTAVLTSAFRTFTNAYLTTKDVHSLTMSFDTDVRKTVLSAYFGALA"
  },
  {
    "reference": "MYPVQNNNMTKFIFVTGGVTSSLGKGIISASLAKLLQARGYKVTIQKFDPYINIDPGTLNPYEHGECFVTEDGAETDLDLGHYERFLNIPTSQANNVTTGRIYQHVINQERQGAFLGKTVQVVPHITDEIKRRMKLLDQDNKYDIIITELGGTVGDIESLPFIEAVRQLRWELGSNKSLVIHLTLVPFLAAAGELKTKPTQHSVKTLLEYGIQPDILVCRTEHHLNQEIRKKLALFCNVHVNAVIESIDAPSIYDVPLLMLKEQLDKIVLSKLKLTARQEPDLETWKTFLGKLKNPTAEVNIALVGKYIELPDAYKSINEAFIHAGAANEAKVRVKCLAAESINEENVAEVLQGFSGVLVAPGFGERGLAGKIATIQYVREHDIPFFGICLGMQVAVIEFARHVLGLKDANSFEMDQKTKHPVINLMETQKSIKEMGGTMRLGAYPCTIKKGTRAAAIYGKTHITERHRHRYEFNNDYLKTFEKAGMIASGINPDNGLVEIIELKNHPFFVGGQFHPELKSTVANPHPLFVNFVAATLAFTKKNKK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must have at least one CTP synthase N-terminal, Glutamine amidotransferase domain.2. The active site, Nucleophile active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. The CTP, Mg(2+), ATP binding site should be located in a region of the protein that is accessible to the ligand.4. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.. The designed protein sequence is ",
    "response#1": "LEDAYLSIHESLKHAGFETNTNVNVLWVDADEMDDAHADRLERADGIIVPGGFGARGTSGKIEAVRYARENDVPFLGLCLGFQMAVIEFARNVCGYEGAHSAEMDETTAHPVIDILPEQYEVEDMGGTMRLGAHETDIDTATLAHEVYGETSCTERHRHRYEVNPEYIEELEAAGLVFSGRAGNRMEILELPDNEFFFGTQFHPEFRSRPGRASPPFVSLR",
    "response#2": "LKDAYKSLQEALVHGGIANNVRVNITWLASEIFEQDHEAILALEDVNAILVPGGFGERGTSGKIAAVQFARERKVPYLGICYGMQMAVIEAARNLAGLKDANSTEIGPTRNPVVGLMTEWVRGNRTELRQEGGDLGGTMRLGAYDAVLSEGSRVAEIYGTRLISERHRHRYEVNPAYRRHLEEHGVLFSGMSPDGILPEVIEIKDHPWFIGVQFHPELKSKPLDPHPLFSSFVEDAYKSINEALTHGGFANRVKVRPEWIASERLEREDPAQVLEGFSGILVPGGFGERGTEGKIRAAEFARTHGVPYLGICLGMQMAVIEAARNLTDLADAGSEEFDHEAPPGADPRRFTPVVYHLKEWVQGNHVVERKVTDDKGGTMRLGAYDAVLADGSKVAEVYDGAPRIEERHRHRYEVDVRYRDALEANGLRFTGMSPDGRLPEIVEAAGHPWYIGVQFHPELKSKPFDPHPLFEGFVEWTRL",
    "response#3": "DSYKSIVEALIHGGAANDAKVNIKWVQSETLNAQNIEKYLGDVSGILVAPGFGDRGVEGKFRAVKYARENEIPFFGICLGMQSAVVEFARNVAGMEGANSTEFVKDTEFPVIDLMLEQKNVKDKGGTMRLGKYACSLKKGTKAALAYGIDSVDERHRHRYELNNALREDLQSAGLVVSGVNPEKDLVEIVELADHPWFVGVQFHPELRSTVYNPHPLFISFG"
  },
  {
    "reference": "MAQAPADPGREGHLEQRILQVLTEAGSPVKLAQLVKECQAPKRELNQVLYRMKKELKVSLTSPATWCLGGTDPEGEGPAELALSSPAERPQQHAATIPETPGPQFSQQREEDIYRFLKDNGPQRALVIAQALGMRTAKDVNRDLYRMKSRHLLDMDEQSKAWTIYRPEDSGRRAKSASIIYQHNPINMICQNGPNSWISIANSEAIQIGHGNIITRQTVSREDGSAGPRHLPSMAPGDSSTWGTLVDPWGPQDIHMEQSILRRVQLGHSNEMRLHGVPSEGPAHIPPGSPPVSATAAGPEASFEARIPSPGTHPEGEAAQRIHMKSCFLEDATIGNSNKMSISPGVAGPGGVAGSGEGEPGEDAGRRPADTQSRSHFPRDIGQPITPSHSKLTPKLETMTLGNRSHKAAEGSHYVDEASHEGSWWGGGI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should contain one or more Z-binding domains that are essential for its function.2. The ideal protein would be one that plays a critical role in activation of innate immune response, defense response to virus, positive regulation of apoptotic process, regulation of interleukin-1-mediated signaling pathway, defense response to fungus through double-stranded RNA binding, DNA binding, identical protein binding within cytosol, cytoplasm, nucleus.3. The designed protein should contain at least one RIP homotypic interaction motif (RHIM) motif to achieve its intended function.4. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "WGRLFIRMYYNGEQVHELLTTSQSGCRISSALRRDPAVHYCAVGSPGQVWLPNVPNLACEIAKRELCDTLDACAKGILLTSSCNGIFCVCYHNGPVHFIGNTVPPDSGPLLLPQGKPTRIFNPNTFLVGLANSPLPAPSHVTCPLVKLWLGKPVAVGKLEPHAC",
    "response#2": "WGRLFIRMYYNGEQVHELLTTSQSGCRISSALRRDPAVHYCAVGSPGQVWLPNVPNLACEIAKRELCDTLDACAKGILLTSSCNGIFCVCYHNGPVHFIGNTVPPDSGPLLLPQGKPTRIFNPNTFLVGLANSPLPAPSHVTCPLVKLWLGKPVAVGKLEPHAC",
    "response#3": "WGRLFIRMYYNGEQVHELLTTSQSGCRISSALRRDPAVHYCAVGSPGQVWLPNVPNLACEIAKRELCDTLDACAKGILLTSSCNGIFCVCYHNGPVHFIGNTVPPDSGPLLLPQGKPTRIFNPNTFLVGLANSPLPAPSHVTCPLVKLWLGKPVAVGKLEPHAC"
  },
  {
    "reference": "MSAGERASNDDTTNFGFERVALDEKQGRVDDVFRSVAKRYDLMNDLMSAGLHRAWKAQLIAMLRPPQGRAFRHLDVAGGTGDIAFRSLAAGGPGTHVTVLDINEAMLRVGAERAGHRFDGRIDFVTGNAETLPLPAESFDAYTIAFGIRNVPRIDAALKEAHRVLKPGGRFLCLEFSKVDLPVLDRIYDAYSFHMIPRLGARVAGDADSYRYLVESIRQFPTPGAFAGMIEAAGFRHVTHRLLSGGIVAIHSGWKIS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with demethylmenaquinone methyltransferase activity, S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity functions is required to enable menaquinone biosynthetic process.2. The protein must exhibit the following characteristics: Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2).. The designed protein sequence is ",
    "response#1": "MLKITKRFGFTLIELLTVIAIIGILAAIAI",
    "response#2": "MENTIIVGSDHAGFDVKETAKKHL",
    "response#3": "MAQNQFSYRKT"
  },
  {
    "reference": "MDNNQSNTPKPKFNAFWVYALIAIVFIALQYFQFGKHPHEIFMKDLREMIAAKDVDKIEVINKEVARVYLKPGRLDHYQKLVGKDFNRIPKDGPHFELQIGDVTAFKDEIDKVQKDFAPADYVNIVYKVEKNYFLDIFISFILPFLILFAIWGYFYRRMSGGGNVGPGGTNIFNVGKSKAKIFDRDTSVKVTFADVAGLEEAKIELKEIVDFLKNPKKYTDLGGKIPRGALLVGPPGTGKTLLAKAVAGEANVPFFSLSGSDFVEMFVGVGASRVRDLFAQAKQKAPCIVFIDEIDAVGRSRSKNPAWTSNDERENTLNQLLTEMDGFAPNTGIIVLAATNRADILDRALLRAGRFDRQIHVELPDLKERLEIFKVHMRPLKLANDIDVEFLAKQTPGFSGADIANVCNEAALIAARKNKTSIEKQDFLDAIDRIIGGLEKRNKIISKLEKKTIAYHEAGHATVSWLLEHANPLVKVTIIPRGKALGAAWYLPEERQITTTEQLYDEMCATLGGRAAEEVIFGKVSTGALNDLERVTKQAYAMVAYFGMSPKIGNISYYDSSGQSEFTFSKPYSEKTAELIDEEAKKIVEAQYQRAKEILRENKEGLDQLAQRLLEKEVIFSEDLERIFGKRKGGVPEEQQLLIENGTSSDNTTQQPENTQTE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Plays a role in the quality control of integral membrane proteins.; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins2. The designed protein must have a active site that is structurally stable and resistant to denaturation.3. The designed protein should contain one or more AAA+ ATPase domains that are essential for its function.4. A protein that contributes to proteolysis, protein catabolic process by functioning in plasma membrane with zinc ion binding, ATP binding, ATP-dependent peptidase activity, metalloendopeptidase activity, ATP hydrolysis activity.5. The protein design should emphasize Polar residues compositional bias.6. Zn(2+) is required for the proper folding and stability of the protein.7. The protein should contain a Helical membrane-spanning region.8. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding. The designed protein sequence is ",
    "response#1": "MVMSEEEKLKTAYHESGHAIVGRSLEEHDPVYKVTIIPRGRALGVTMFLPEEDKYSYSHQSILDRICGLYGGRIAEELIYGEKGVTTGASNDIERATELAHNMVTKWGLSEKLGPIKYGDETDEPFLGRSAGASKQGISDDTAHEIDNEVKTILSNCYQHAKDILSENIEALHAMSKALMDYETLDVDQIDDQTMEILKQVKEKEKKKKQN",
    "response#2": "MVMSEEEKLKTAYHESGHAIVGRSLEEHDPVYKVTIIPRGRALGVTMFLPEEDKYSYSHQSILDRICGLYGGRIAEELIYGEKGVTTGASNDIERATELAHNMVTKWGLSEKLGPIKYGDETDEPFLGRSAGASKQGISDDTAHEIDNEVKTILSNCYQHAKDILSENIEALHAMSKALMDYETLDVDQIDDASNAM",
    "response#3": "VLLVGPPGTGKTLLAKAVAGEANVPFISISGSEFVEMYVGVGAARVRDLFERAKKMSPCIIFIDEVDAVGRKRGIRAGGGTEEREQTLNQILVEMDGFNSETNVIVIAATNRVDVLDPAFLRPGRFDRQVTVELPFRESLETVKAEIIARGTPGMAGADLANLVNEAALFAARAGKRLVDMDDFKLANDYSNVDGIIRNLQKCCSPWLILTPEEKRKVAFHEAGHATVSWFLEHANPLLKVSIIPRGRSLGAAWYVPEERQLNTTEQMFHEMCALLGGRASEEVVFSEISTGALNDLERVNKMAYAMVAYYGMSDDLVNMCYFDSSGESQMSFSKPYSEKTAEEIDGEVRALIASAYARAKEILVEHRAGLDQLAERLLQEEVIFSDDLVRFLDVAFE"
  },
  {
    "reference": "MQRLVLASNNPGKLREFSALLSPLGFDVVPQGELGVPEAEEPFATFVENALTKARHAARLTGMPALADDSGICVEALDGAPGVYSARYAQTVGKPKSDAANNAHLISQLAGKLNRRAHYYCVLVMVRHADDPRPIIAEGIWAGEVVDAPRGNGGFGYDPHFLLPSVGKTAAELSAEEKNAISHRALALQELVARLQTAGIKSCA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP2. A protein with dITP diphosphatase activity, XTP diphosphatase activity, metal ion binding is needed to enhance purine nucleoside triphosphate catabolic process, nucleotide metabolic process in a specific context.3. The Proton acceptor of the protein must be engineered to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MSLPNHPLILASGNAHKVRELEALLLPFGIAVRPQADLGVDEADEPHMTFVENALAKARHASTITGLPALADDSGICVDALGGAPGLYSARYADGHGDAANNAKLLAELAGVTDRRAYYYCVIVLVRHADDPEPLIAEGRWHGEILAEPRGEGGFGYDPLFHVPALGKTAAELTREEKNAISHRGQALRAMLVRLKG",
    "response#2": "MTRKIVLASGNAGKLRELSALLAPLGIEVLPQSAFNVSEAEEPHPSFVENALAKARHASRHTGLPALADDSGLCVEALGGAPGVLSARYAGEPKSDARNNAKLLRELAGVPAAQRTAHFHCVLVLVRHADDPQPLIAEGRWHGEILDAPRGAGGFGYDPLFWLPAFGCTAAELPAEHKNAVSHRGQALRMLLQQLRQR",
    "response#3": "MTIFDLNRKK"
  },
  {
    "reference": "MENQNHSEDTTHFGYKTVNTEEKVTKVAEVFHSVASKYDMMNDLMSMGVHRIWKRLTIEKSGVRRGHSVLDLAGGTGDLTAKFSRMVGDTGQVILGDINDSMLKVGRARLLDKGIVGNVDYVQANAECLPFPDNSFDCITIAFGLRNVTDKDAALASMFRVLKPGGRLLILEFSRPVAPGLSPLYDFYSFNILPFMGKIVANDSESYRYLAESIRMHPDQETLKDMMAAAGFERCEYNNLTGGIVALHRGFKF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity, S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity. The designed protein sequence is ",
    "response#1": "GTIVTL",
    "response#2": "NYAK",
    "response#3": "GHPK"
  },
  {
    "reference": "MAFDLPNDFRCPISLEIMSDPVILQSGHTFDRVSIQQWIDSGNRTCPITKLPLSETPYLIPNHALRSLILNFAHVSLKESSRPRTQQEHSHSQSQALISTLVSQSSSNASKLESLTRLVRLTKRDSSIRRKVTESGAVRAALDCVDSCNQVLQEKSLSLLLNLSLEDDNKVGLVADGVIRRIVTVLRVGSPDCKAIAATLLTSLAVVEVNKATIGSYPDAISALVSLLRVGNDRERKESATALYALCSFPDNRKRVVDCGSVPILVEAADSGLERAVEVLGLLVKCRGGREEMSKVSGFVEVLVNVLRNGNLKGIQYSLFILNCLCCCSGEIVDEVKREGVVEICFGFEDNESEKIRRNATILVHTLLGIPMSS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. We require a protein that can specifically bind to the metabolites produced in the protein ubiquitination, Protein modification pathway.2. The designed protein should have ubiquitin-protein transferase activity within nucleus, cytoplasm to regulate protein ubiquitination.3. The protein contains novel U-box domains that confer a unique function or activity.4. For general function, the protein need meet that Functions as an E3 ubiquitin ligase ; Involved in the age-dependent pseudo-self-compatibility process. The designed protein sequence is ",
    "response#1": "LGPFFSYGLESSARRPNHRVFVDCEEDARKYDGSVNTEQKLYFQRMDPIRTMLHQLMLPLASDQGSRNKTLRWIATIISTNDIRTRSHYDPSDVLCDHYMTNFLSVMYMFSEKIDLSKIIVDYPFLPSSLINISKETRLKMDESGAVAFASQFADRPDEYHFSTVCFFLTIAAQRLVIPPLMNQISEYSRHLKELKHKINALKEKLNTVSGFERAEVEKKLNYETEHWKLMSRHLLCVKTQAQDPALMASSMDFVDKQMKFILNLLCDNLDLLGDDSQLPTEVSQMFCALPEYFLEDALDFYIFAISNGMKLLMERNADWISRLTVLFTQYHYIKSPFLVSKLVRVLSSIQPPLWFNVVRLRMAQENLLMCMIKFYSDFEDNGDFYEKFNVRGNIQYMLEKMEEDMFYKGKFMDMARECGAEFIRFVNMVINDATWCIDESLSGLKSIHDVEKKMANKVEWDNTDQEIRNQDLGVYEEAKRKVKGV",
    "response#2": "MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLK",
    "response#3": "LGPFFSYGLESSARRPNHRVFVDCEEDARKYDGSVNTEQKLYFQRMDPIRTMLHQLMLPLASDQGSRNKTLRWIATIISTNDIRTRSHYDPSDVLCDHYMTNFLSVMYMFSEKIDLSKIIVDYPFLPSSLINISKETRLKMDESGAVAFASQFADRPDEYHFSTVCFFLTIAAQRLVIPPLMNQISEYSRHLKELKHKINALKEKLNTVSGFERAEVEKKLNYETEHWKLMSRHLLCVKTQAQDPALMASSMDFVDKQMKFILNLLCDNLDLLGDDSQLPTEVSQMFCALPEYFLEDALDFYIFAISNGMKLLMERNADWISRLTVLFTQYHYIKSPFLVSKLVRVLSSIQPPLWFNVVRLRMAQENLLMCMIKFYSDFEDNGDFYEKFNVRGNIQYMLEKMEEDMFYKGKFMDMARECGAEFIRFVNMVINDATWCIDESLSGLKSIHDVEKKMANKVEWDNTDQEIRNQDLGVYEEAKRKVKGCV"
  },
  {
    "reference": "MQINGITPQALTAYGIHDVRDIVYNPSYELLFEEERSPTLQGYERGIETQLGAVAVDTGIFTGRSPKDKYIVRDDVTRDTVWWSDQGKGKNDNHPLSQETWTHLKQLVTTQLSGKRLFIIDAFCGANPDSRLSVRFVTEVAWQAHFVKNMFIRPSDEELEGFEPDFIVMNGAKCTNPNWQEQGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSIMNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGVFNFEGGCYAKTIKLSKEAEPDIYGAIKRDALLENVTVLADGTVDFNDGSKTENTRVSYPIYHIQNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTSDQTQYHFLSGFTAKLAGTERGVTEPTPTFSACFGAAFLMLHPTQYAEVLVKRMKAAGAQAYLVNTGWNGSGKRISIKDTRGIIDAILNGSIDDAEMQTLPVFDLAIPTSLPGVNPDILDPRDTYASVEQWQEKADDLAQRFITNFDKYTDAPAGAALVKAGPKR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should take into account the physiological conditions of the Carbohydrate biosynthesis pathway.2. The designed protein must possess metal ion binding, ATP binding to support gluconeogenesis under certain conditions. The designed protein sequence is ",
    "response#1": "LPERTSDLAFNASVQRHLSAGMQAYVNDAFLGKDGPRGRNFNMRWLGAAVGDMHRIMQRGGLFFYVNDSRPGYEKGRLRLVYEANPIAFLAREAGGKATDGSRPILDIVPQTYHERSALVFGVAEEVDILR",
    "response#2": "RIGVVLSGGQAPGGHNVIAGLFDYLQKHAPGSLLLGFRGGPAGIMRCKYVEITSEFLYPYRNQGGFDIICSGRDKIETPEQFKQAEDTAVKLDLDGIVVIGGDDSNTNACLLAEYFRARNLKTHVIGCPKTIDGDLKSKEVPISFGFDTACKIYAEMIGNIMVDARSTGKYYHFVRLMGRAASHITLECALQTHPNVALIGEEVSVKKQTLRQVTNSITDTICKRAENGQHYGVILIPEGLIDFIPEIQHLISELNEILASGTVDEEGHWRHKLSPQCHALFDSLPHSIQEELLLERDPHGNVQVAKIETEKMLISMVETELLRRKQEGSYTGNFKGQSHFLGYEGRCGLPTNFDSTYCYALGYAHAGKTGYMAALSGISCVPPSTKEFAINASNARHWDAPVKAYIEDCQRGTEGPRDKDYNMRWLASLVADIQRVLTRGGVFLYPGDARKNYARGRLRLLYEVAPVAMLVEQAGGAATDGQTRILDIVANGIHERAPLVCGSTEEVTCVATYS",
    "response#3": "LPERTSDLAFNASVQRHLSAGMQAYVNDAFLGKDGPRGRNFNMRWLGAAVGDMHRIMQRGGLFFYVNDSRPGYEKGRLRLVYEANPIAFLAREAGGKATDGSRPILDIVPQTYHERSALVFGVAEEVDILY"
  },
  {
    "reference": "MERLQRLMMNTKVGAADANKDDTKETVYISSIALLKMLKHGRAGVPMEVMGLMLGEFVDEYTVNVVDVFAMPQSGTGVSVEAVDDVFQARMMDMLKQTGRDQMVVGWYHSHPGFGCWLSSVDVNTQKSFEQLNNRAVAVVVDPIQSVKGKVVIDAFRLIDTGALINNQEPRQTTSNSGLMNKANIQALIHGLNRHYYSLNIDYHKTPAETKMLLNLHKEQWQSGLKMQDYQEKETENLEATKRMVAVAQQYSKRIEEEKELSEEELKTRYVGKQDPKKHLAKTAENTLEENTVSVLTAGVNSIAIK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design must be able to perform metal ion binding in order to contribute to mitochondrial fission. The designed protein sequence is ",
    "response#1": "LKDAYKSLNEALFHGGIANRVKVNLEWIESEVFEREDPAPYLENVHGILVPGGFGERGSEGKILAARFARERKVPYFGICFGMQMAVIEAARDLVGIEHAGSTEFGPTDEPVVGLMTEWMRGNELEKRKANGDLGGTMRLGAYPAALAKGSKVASIYGSNLISERHRHRFEVNMRYRERLEKAGVLFCGLSPDGLLPEIIELPDHPWFIGVQFHPELKSRPFEPHPLFKSFIF",
    "response#2": "VTIAIVGKYTGLKDAYKSLNEALFHGGIANRVKVNLEWIESEVFEREDPAPYLENVHGILVPGGFGERGSEGKILAARFARERKVPYFGICFGMQMAVIEAARDLVGIEHAGSTEFGPTDEPVVGLMTEWMRGNELEKRKANGDLGGTMRLGAYPAALAKGSKVASIYGSNLISERHRHRFEVNMRYRERLEKAGVLFCGLSPDGLLPEIIELPDHPWFIGVQFHPELKSRPFEPHPLFKSFL",
    "response#3": "LKDAYKSLNEALFHGGIANRVKVNLEWIESEVFEREDPAPYLENVHGILVPGGFGERGSEGKILAARFARERKVPYFGICFGMQMAVIEAARDLVGIEHAGSTEFGPTDEPVVGLMTEWMRGNELEKRKANGDLGGTMRLGAYPAALAKGSKVASIYGSNLISERHRHRFEVNMRYRERLEKAGVLFCGLSPDGLLPEIIELPDHPWFIGVQFHPELKSRPFEPHPLFKSFIN"
  },
  {
    "reference": "MRAKGIQKNYQQWWIWGILGFWMLMSCNGKDEDLWVTVYYGVPVWKEAKTTLFCASDAKAYKKEVHNVWATHACVPTDPNPQEIVLGNVTENFNMWENDMVDQMHEDVISLWDQSLKPCVKLTPLCVTLNCTKANVTLENKTNSTNGTNITAPRLLDDIKNCSFNATTEISDKKKKEYALFYRLDIVPLDGDKNDSTEYRLINCNSSTITQACPKVSFDPIPIHYCAPAGYAILKCNNETFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVIIRSKNISDNAKTIIVQLNESVAIVCTRPGNNTRRSVRIGPGYAFLTNKIIGNPRQAHCNISAKEWHEALRRVGQKLSKHFPNKTIAFNSSSGGDPEITTHSFNCRGEFFYCNTSQLFNSSLFWNGTYNSTYNSTGNTNSTITIQCRIKQIINMWQEVGQAMYAAPIAGNITCNSNITGLLLTRDGGDTTNGTEIFRPGGGDMRDNWRSELYKYKVVEVQPLGVAPTKAKRRVVGREKRAVPILGAVFLGILGAAGSTMGAASMALTVQTRQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLQDQQLLGLWGCSGKLICTTNVPWNSSWSNKSRTDIWDNMTWMQWDKEISNYTGIIYHLLEDSQNQQENNEKELLELDKWNSLWTWFDITKWLWYIKIFIMIVGGLIGLRIIFTVISLVNRVRQGYSPLSFQTLIPNPRGPDRPGGIEEEGGEQDKSTSIRLVNGFLALAWDDLR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that contributes to positive regulation of plasma membrane raft polarization, fusion of virus membrane with host plasma membrane, actin filament reorganization, virion attachment to host cell, positive regulation of establishment of T cell polarity by functioning in virion membrane, plasma membrane, viral envelope, host cell plasma membrane, host cell endosome membrane with structural molecule activity.2. The protein must exhibit the following characteristics: The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Transmembrane protein gp41: Acts as a class I viral fusion protein; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.3. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "MKVKGIRKNYQHLWRWGTMLLGMLMICSATEKKWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLENVTESFNMWKNNMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTHNVTATNATNGTISSGEGELKNCSFNITSSIRNKMKKEYALFYTLDIIPIDDDSNTTDSNTTYRLRSCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSKNFSDNAKTIIVQLNESVVINCTRPNNNTRKSITIGPGRAFYTTGQIIGDIRQAHCNLSRTQWNNTLKQIVGKLREQFGNNKTIVFNQPSGGDPEIEMHSFNCGGEFFYCNTTQLFNSTWIFNSTWNDTNTEGNDTITLQCRIKQFINMWQEVGKAMYAPPISGQIRCSSNITGLLLTRDGGINESRTNETFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRAVGIGAVFLGFLGAAGSTMGAASIALTAQARQLLSGIVQQQSNLLRAIEAQQHMLRLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTNVPWNSSWSNKSQSEIWDNMTWLQWDKEISAYTDTIYNLLEKSQNQQEKNEQDLLALDKWANLWNWFDISRWLWYIKIFIMIVGGLIGLRIVFAVLSIINRVRQGYSPLSFQTRFPAQRGPDRPEGTEEEGGDRDRGGSTRLVHGFLALIWDDL",
    "response#2": "MWVTVYYGVPVWRDADTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVKQMHKDIISLWDQSLQPCVKLTPLCVTLNCNRVNNSSSSNSTNNXXTSSNSTVSPGIKXEMMNCSFNMTXXVXXKKXKVSALFYRLDVXQIDSEQTNSTXYRLINCNTSTVTQACPKVTFEPIPIHYCAPAGFAILKCKDKNFTGTGPCXNVSTVQCTHGIRPVXTTQLLLNGSLAEERVMIRSENLTNNTXTILVQFQNPVRINCTRPSNNTRKSLRIGPGQTFYATGEVIGDIRRAHCNVSTQDWNDTLKEVVTQLRGHFKNKTIIFKNHAGGDLEIVTHSFNCGGEFFYCNTTDLFNSTWDKDGNGTVSSGSNGTITLQCRIKQIINMWQRVGQAMYAPPIQGVIRCDSNITGLLLTRDGGNGNDSQANETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVQREKRXMAGFLGAMFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLKAIEAQQHLLQLTVWGIKQLQARVLALERYQRDQQLLGLWGCSGKIICTTNVPWNSTWSNKSYEEIWNNMTWIEWEREISNYTGQIYAILTEAQDQQDRNEKDLLALDQWTSLWNWFSITHWLWYIKIFIMIVGGLIGLRIVFAVLSIVNRVRQGYSPLSLQTRFPAQTRE",
    "response#3": "MWVTVYYGVPVWRDADTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVKQMHKDIISLWDQSLQPCVKLTPLCVTLNCNRVNNSSSSNSTNNXXTSSNSTVSPGIKXEMMNCSFNMTXXVXXKKXKVSALFYRLDVXQIDSEQTNSTXYRLINCNTSTVTQACPKVTFEPIPIHYCAPAGFAILKCKDKNFTGTGPCXNVSTVQCTHGIRPVXTTQLLLNGSLAEERVMIRSENLTNNTXTILVQFQNPVRINCTRPSNNTRKSLRIGPGQTFYATGEVIGDIRRAHCNVSTQDWNDTLKEVVTQLRGHFKNKTIIFKNHAGGDLEIVTHSFNCGGEFFYCNTTDLFNSTWDKDGNGTVSSGSNGTITLQCRIKQIINMWQRVGQAMYAPPIQGVIRCDSNITGLLLTRDGGNGNDSQANETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVQREKRXXXXXXXLWVTVYYGVPVWKDATTPLFCASDAKAYVTEGHNVWATHACVPTDPNPQEVVMENVTENFNMWKNDMVEQMHEDVISLWDESLKPCVKLTPLCVTMNCTDANLNTNSSINNSSLIKMKEEIKNCSFNITSQIKNTVRKDYALFNKLDIVPIENDNSSYMLINCNTSVITQACPKVSFEPIPIHYCTPAGFAIIKCNEKGFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEGDIVIKSENFTNNAKTIIVQLNETVIINCTRPNNNTRKSIHVGWGRSLYATGDIIGNIRQAHCNVSAERWSNILKQVAIKLKDQFKPKTIDFKQPTGGDPEIVMHTFNCGGEFFYCNTSKLFNYTLTNNTWNNQTVNGTDVITLPCRIKQIINRWQEVGKAMYAPPIRGQINCTSNITGLLLTRDGGNGNETGSNRSEIFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREK"
  },
  {
    "reference": "MEVKMDELVLDVDVEEATGSELLVKSEPEADLNAVKSSTDLVTVTGPIGKNGEGESSPSEPKWLQQDEPIALWVKWRGKWQAGIRCAKADWPLTTLRGKPTHDRKKYCVIFFPHTKNYSWADMQLVRSINEFPDPIAYKSHKIGLKLVKDLTAARRYIMRKLTVGMFNIVDQFPSEVVSEAARDIIIWKEFAMEATRSTSYHDLGIMLVKLHSMILQRYMDPIWLENSFPLWVQKCNNAVNAESIELLNEEFDNCIKWNEVKSLSESPMQPMLLSEWKTWKHDIAKWFSISRRGVGEIAQPDSKSVFNSDVQASRKRPKLEIRRAETTNATHMESDTSPQGLSAIDSEFFSSRGNTNSPETMKEENPVMNTPENGLDLWDGIVVEAGGSQFMKTKETNGLSHPQDQHINESVLKKPFGSGNKSQQCIAFIESKGRQCVRWANEGDVYCCVHLASRFTTKSMKNEGSPAVEAPMCGGVTVLGTKCKHRSLPGFLYCKKHRPHTGMVKPDDSSSFLVKRKVSEIMSTLETNQCQDLVPFGEPEGPSFEKQEPHGATSFTEMFEHCSQEDNLCIGSCSENSYISCSEFSTKHSLYCEQHLPNWLKRARNGKSRIISKEVFVDLLRGCLSREEKLALHQACDIFYKLFKSVLSLRNSVPMEVQIDWAKTEASRNADAGVGEFLMKLVSNERERLTRIWGFATGADEEDVSLSEYPNRLLAITNTCDDDDDKEKWSFSGFACAICLDSFVRRKLLEIHVEERHHVQFAEKCML",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a fast turnover rate for L-lysyl-[histone] + S-adenosyl-L-methionine = H(+) + N(6)-   methyl-L-lysyl-[histone] + S-adenosyl-L-homocysteine.2. A protein that contributes to negative regulation of gene expression, epigenetic, regulation of histone H3-K9 dimethylation by functioning in nucleus with sequence-specific DNA binding, histone lysine N-methyltransferase activity, DNA-binding transcription factor activity, zinc ion binding.3. The protein contains novel Post-SET, Pre-SET domains that confer a unique function or activity.4. For general function, the protein need meet that Mediates H3K9me2 deposition and regulates gene expression in a DNA methylation-independent manner; May represent a novel mechanism for plants to regulate their chromatin and transcriptional state, which may allow for the adaptability and modulation necessary to rapidly respond to environment or developmental cues.5. The Zn(2+), S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions.6. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "LPDMTSGVESIPVSLVNEVDTDNGPAYFTYSTTVKYSESFKLMQPSFGCDCANLCKPGNLDCHCIRKNGGDFPYTGNGILVSRKPMIYECSPSCSVRLQVFKSEKKGWGVRCLDDIDKGTFVCIYSGRLLRRATPEKTNIGENGREQQHIVKNSFSKKRKLEVVCSDCDAHCDSPKAEDCPPKLSGDLKEPAVEMNHRNISRTQHHSVIRRTKSKTTVFHYSEKNMGFVCSDSAAPEDKNGFKPAQEHVNSEARRAHEDLSSNPAGDSEDTQLTESDVIDITASREDSAPAYRCKHATIVDRKDTKQVLEVPGKKSQEEEPAASQSQQALCDEELPSERTKIPSASLMQLSKESLFLLDASKEGNVGRFLNHSCCPNLWVQNVFVETHDRNFPLVAFFTNRYVKARTELTWDYGYEAGALPCNRYSSEQNGAKIVATKEWKRNDKIELLVGCIAELSEIEENMLLRHGENDFSVMYSTRKNCAQLWLGPAAFINHDCRPNCKFVSTGRDTACVKALRDIEPGEEISCYYGDGFFGGEGLYAKCDVDQDTVMAFYNGVRLKQDEVENRDWSQNSNTISLTDDIAIDVPEEYVSTDNYCASLGHKVNHSFDPNCRYDIYQHPRFGFIKCVRTIRGVSEGDELTVHCSAVRTVCKCGAVTCRGYLPVRLQVFKSEKKGWGVRCLDDIDKGTFVCIYSGRLLRRATPEKTNIGENGREQQHIVKNSFSKKRKLEVVCSDCDAHCDSPKAEDCPPKLSGDLKEPAVEMNHRNISRTQHHSVIRRTKSKTTVFHYSEKNMGFVCSDSAAPEDKNGFKPAQEHVNSEARRAHEDLSSNPAGDSEDTQLTESDVIDITASREDSAPAYRCKHATIVDRKDTKQVLEVPGKKSQEEEPAASQSQQALCDEELPSERTKIPSASLMQLSKESLFLLDASKEGNVGRFLNHSCCPNLWVQNVFVETHDRNFPLVAFFTNRYVKARTELTWDYGYEAGA",
    "response#2": "LPDMTSGVESIPVSLVNEVDTDNGPAYFTYSTTVKYSESFKLMQPSFGCDCANLCKPGNLDCHCIRKNGGDFPYTGNGILVSRKPMIYECSPSCSNGVESVSIPFCNEIDNSKLPRFKYRNTVWPRIYHLNFSNMFSDSCDCSEGCIDIKKCACLQLTAKNAKACPLSSDGECAGYKYKRLQRLIPTGIYECNLLCKCNKQMCQNL",
    "response#3": "LPDMTSGVESIPVSLVNEVDTDNGPAYFTYSTTVKYSESFKLMQPSFGCDCANLCKPGNLDCHCIRKNGGDFPYTGNGILVSRKPMIYECSPSCVRLQVFKSEKKGWGVRCLDDIDKGTFVCIYSGRLLRRATPEKTNIGENGREQQHIVKNSFSKKRKLEVVCSDCDAHCDSPKAEDCPPKLSGDLKEPAVEMNHRNISRTQHHSVIRRTKSKTTVFHYSEKNMGFVCSDSAAPEDKNGFKPAQEHVNSEARRAHEDLSSNPAGDSEDTQLTESDVIDITASREDSAPAYRCKHATIVDRKDTKQVLEVPGKKSQEEEPAASQSQQALCDEELPSERTKIPSASLMQLSKESLFLLDASKEGNVGRFLNHSCCPNLWVQNVFVETHDRNFPLVAFFTNRYVKARTELTWDYGYEAGALPCNRYSSEQNGAKIVATKEWKRNDKIELLVGCIAELSEIEENMLLRHGENDFSVMYSTRKNCAQLWLGPAAFINHDCRPNCKFVSTGRDTACVKALRDIEPGEEISCYYGDGFFGLPDMTSGVESIPVSLVNEVDTDNGPAYFTYSTTVKYSESFKLMQPSFGCDCANLCKPGNLDCHCIRKNGGDFPYTGNGILVSRKPMIYECSPSCKDVDISNGVESVSIPFCNEIDNSKLPRFKYRNTVWPRIYHLNFSNMFSDSCDCSEGCIDIKKCACLQLTAKNAKACPLSSDGECAGYKYKRLQRLIPTGIYECNLLCQRLLEVYKDVVKGRGIRTKVNFEKGDFVVEYRGVMMEYSEAKVIEEQYSNDEEIGSYMYFFEHNNKKWCIDATKESPWKGRLINHSVLRPNLKTKVVEIDGSHHLILVARRQIAQGEELLYDY"
  },
  {
    "reference": "MTVNLTLQHASEIIGQDNVDLTLAAGASAKVSNLTVASEWLTNNTGYLVTISVNDKSGNVLSSKRAGLSVEDDWTVFPRYGIVAGSPTDQNSILVKNLEAYRKELELMKSMNINSYFFYDAYNEATDPFPEGVDSFVQKWNTWSHTQVDTKAVKELVDQVHKSGAVAMLYNMISADSNPKNPALPLAALAYNFYDSFGKKGEPMTYTIGDNPTQVYYDPANPDWQKYIAGVMKSAMDRMGFDGWQGDTIGDNRVTDYEHRNSTDEADSHMMSDSYASFINAMKDLIGEKYYITINDVNGGNDDKLAKARQDVVYNELWTNGGSVIPGRMQVAYGDLKARIDMVRNKTGKSLIVGAYMEEPGIDYTVPGGKATNGAGKDALAGKPLQADATLLVDATVAAAGGYHMSIAALANANAALNVLQSAYYPTQYLSVAKDTIRKLYNYQQFITAYENLLRGEGVTNSTQAVSTKNASGEILSKDALGVTGDQVWTFAKSGKGFSTVQMINMMGINAGWHNEEGYADNKTPDAQENLTVRLSLAGKTAQEAAKIADQVYVTSPDDWATSSMKKAQASLETDENGQPVLVISVPKLTLWNMLYIKEDTTATPVEPVTNQAGKKVDNTVTSEASSETAKSENTTVNKGSEAPTDTKPSVEAPKLDETTKPAPSVDELVNSAAVPVAIAVSETAHDKKDDNSVSNTDQGTVASDSITTPASEAASTAASTVSSEVSESVTVSSEPSETENSSEASTSESATPTTTAISESHAVVEPV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be optimized for dextranase activity activity in order to improve metabolic process. The designed protein sequence is ",
    "response#1": "MVVLPYRDRLEVFSRYLQQLVMESLGKRLDRDGNVVHQGIAVYGNKGSTDQHAYVQQLRDGVDNFFATFIEELEDSEDIPVIKNERPGDFLDGFLQGTRSALTEGGRQNMTITMRCFDERRLGALVALFERAVGLYGELVNVNAYHQPGVEAGKKAAAAIKL",
    "response#2": "GSHLAMSNMFAYGRLAWNPRADSRDIVEEWTRLTFGLDRDVVSTIADMSLKSWPAYEGYSGNLGIQTLTDILYTHYGANPASQDNNGWGQWTRADSKTIGMDRTVSNGTGNAGQYPKEVAARFEHTQTTPDDLMLWFHHVPYTFRLHSGKSVIQHFYDAHYTGAATVQRFPAAWKSLKSKIDTERYNAVLYKLQYQTGHSLVWRDAITEFYRNLSSIPDQLNY",
    "response#3": "GSHLAMSNLYAYGRLAWSPTDDSEQILKDWTRLTFGQNRQVIDTIADMPMTSWPAYENYTGNLGIQTLTDILYTHYGPNPATQDNNGWGQWTRADHNSVGMDRTISNGTGYTGQYPEEVARLYESLETTPDDLVLWFHHVPWTHRLHSGLTVIQHFYNAHYAGSEAAHGFIRQWESLKGLIDRERYEAMRSRLVYQAGHSIVWRDAINNFYYNMTGIPDVAGC"
  },
  {
    "reference": "MMKYVIVTGGVLSGLGKGITASSIGVLMKSVGMKVTSIKIDPYLNSDAGTMSPFEHGEVFVLDDGGEADLDLGNYERFLDVSLTRDHNLTTGKVYSAVIERERRGDYLGKTVQVIPHITNEIQDWIERVSILPTNDSGDAPDACVIELGGTVGDIESSPFVEALRQFQFRVGRQNVCFVHVSLVPVMGPVGEQKTKPTQHTVKELRGLGITPXLIVCRSXDPLHDEVKEKLAAFCHVEPRAVISAHDVSNLYQIPISFERQGVRSMIAECIGVEESDHDEYLEQWREMADRVDXLXEEVXIAMVGKYTGLSXSYLSVIKALQHSAIAVNRKLSIDWIESTDLDSSMLNSDEDSYNAAWEKLKLADGILVPGGFGNRGVEGKVEAARYARENDVPYLGICLGLQIATIEFCRNVLNLEGANSEEFVEDPGSLEHPAVVFMPEISKTHMGGTMRLGAKITPFVVDDCKIKRLYGGLDAVSERHRHRYEVNPDLIDRIEEAGLVYVGKDDTGQRCEIMELMGHPYFVGTQYHPEFKSRPGRPSPPFLGLLKAASGQLE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen2. The protein should have a high affinity for CTP.3. The designed protein must be able to perform metal ion binding, CTP synthase activity under specific conditions. The designed protein sequence is ",
    "response#1": "LQDAYKSIYEAFVHAGAVNECKVIVEPVHSEQLEGTDEALAKRLSEMDGILVAPGFGERGIEGKIRAVKYVRENNIPFFGICLGMQCAVVEYARHVLGIENAASSEVEPDTEHAIIDLMEEQKAVTKKGGTMRLGAYKCELKRKSRAHQIYGTLKIEERHRHRYEFNNKYLADMEKAGLIASGINPESGLVEIVEMKDHPYFVGVQFHPELKSRVEAPHPLFVAFVKAAMEK",
    "response#2": "LPDAYLSVSEAMRAGGFHHDAKVSIRWVASDECRTESGASKALGGVDAILVPGGFGVRGIEGKIGALRWARERGVPTLGICLGLQAMVIEFARNVAGIEGASSTEFDPDGPAPVIATMEEQKAFVDGAGDLGGTMRLGSYAASLEQGSVAATAYEETSVTERHRHRYEVNNAYRDQLAEAGLVFSGTSPDGTLVEFTELPADVHPYYVATQAHPEFKSRPHRAHPLFAGLR",
    "response#3": "LQDAYKSILEAFVHAGAVNECKVEVRNIHSEXKLKNNVRTLFSGLDGILVAPGFGSRGIEGKILAIQYAREXKIPFFGICLGMQCSVIEFARNVLGYPTANSTEMDKHTPYPVIDIMEDQKNIIQMGGTMRLGAYECELKAGSKIHEVYGQEFISERHRHRWEFNNTFLEAFEQAGMKATGRNPKTGLVEVVEIPDRPFFVGSQYHPELKSTVEHPHPLFSGFIR"
  },
  {
    "reference": "MSDTSSHESSTSDDQPKVSFGFEDVSATEKVSRVKGVFRSVASRYDLMNDLMSAGVHRIWKANTIARLNPQPGERILDVAGGTGDVALAFLKSAEEKARRRGQHHLVTRAIVCDINEAMLAAGRSRESLAKYRSVMDWVTGDGMQLPFRDNSVDALSIAFGIRNVADMDMAFREFRRVLKPGGRFACLEFSHMTAGVLQDAYDAYSFNVIPKLGDWITGDAASYQYLVESIRRFPKQDEVTRRITEAGFSQVSCTNYSGGIAALHFGWAV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have a high specificity for S-adenosyl-L-methionine binding, with minimal off-target effects.2. The designed protein should be optimized for S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity, S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity activity in order to improve aerobic respiration, menaquinone biosynthetic process, ubiquinone biosynthetic process, methylation.3. For general function, the protein need meet that Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2).4. Ideal protein should have a binding affinity for key enzymes in the Quinol/quinone metabolism, menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2, menaquinone biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MTATETTHFGFKTVSLEEKVHKVAEVFHSVAAKYDLMNDLMSMGIHRVWKRFTIETANLRKGHKVLDIAGGTGDLTAKFSRMVGETGQVVLADINSSMLNVGRGKLRDKGIVGNVEYVQANAECLPFPDNYFDCITIAFGLRNVTDKDLAIASMLRVLKPGGRLLVLEFSKPTNSLLSKAYDFYSFNILPKIGQLVANDSDSYRYLAESIRMHPDQETLKGMMKDNGFDKVDYHNMTAGVVALHKGYKY",
    "response#2": "MNRIDGHGHILLDSRIETDADGRSIVHIDGLRIAYEEYGQLSADASNRVLRPGGRLLVLEFAASSKSSSYIDPAANLHAIAPDRYDVMNDLMSGGLHRAVTYHRQDDAWRHYAGFLLEQAKAWGVTDNGRRLILGGSGGGFAALVAAAHRPGSYAIVWNPQTDLLAYHAMKLTRYADSYRECMEGAWSEDALAHASPIDRIRALKGETAVMMQNMRGEHWQANHAVPLFAAQAAQGGNPALRLAFAETGFEYHKKWGPHNLPIALRAFAETMVSGDEASLAAALHGAAR",
    "response#3": "MSDNTIEEALIELKNGKMVIVVDDADRENEGDLVMAAEKVTPEAINFMAKYGRGLICLAMEGERLDQLEIPLMVQDNTSKFETAFTVSIEAKHGVTTGISAADRATTILTAVADDAKPEDLARPGHIFPLRAREGGVLRRAGHTEAAVDLARLAGLYPAGVICEVMNEDGTMARLPELMKFAKRFDLKIITIKDLIEYRLRKESLVRKERVLRPGGRLLVLEFPVQPTPNEENYLLTMKEKKLGHLFDKLP"
  },
  {
    "reference": "MDAPRRDMELLSNSLAAYAHIRANPESFGLYFVLGVCFGLLLTLCLLVISISCAPRSRPRTPAPRRDPRSSTLEPEDEDDEEDEDTMTRLGPDDTLQGQELSTEPDGPLSVNVFTSAEELERAQRLEERERILREIWRTGQPDLLGSGTLGPGATATLGRMHYY",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein's functionality will benefit from Polar residues, Acidic residues compositional bias in its structure.2. The designed protein should have a specific function within the membrane. The designed protein sequence is ",
    "response#1": "QSNLQKKFTQLFCGLPSMHSESLGSTFLCTQGVSKNTLKPPYKEPHFLKVSPPIPLPEAAPPPSSTSPNESLDEPQRAQIGGVPFLTLSECKTLEWHLLQRQLQLQWGLPAVIARPPRVQSHTQYKHKPWNKAKPRETLKFFGPGKPFSALTRELFFIPQHARRLLEFHLQKRLIHLRWGLPQRIQRSINMI",
    "response#2": "QSNLQKKFTQLFCGLPSMHSESLGSTFLCTQGVSKNTLKPPYKEPHFLKVSPPIPLPEAAPPPSSTSPNESLDEPQRAQIGGVPFLTLSECKTLEWHLLQRQLQLQWGLPAVIARPPRVQSHTQYKHKPWNKAKPRETLKFFGPGKPFSALTRELFFIPQHARRLLEFHLQKRLIHLRWGLPQRIQRSINMI",
    "response#3": "QSNLQKKFTQLFCGLPSMHSESLGSTFLCTQGVSKNTLKPPYKEPHFLKVSPPIPLPEAAPPPSSTSPNESLDEPQRAQIGGVPFLTLSECKTLEWHLLQRQLQLQWGLPAVIARPPRVQSHTQYKHKPWNKAKPRETLKFFGPGKPFSALTRELFFIPQHARRLLEFHLQKRLIHLRWGLPQRIQRSINMI"
  },
  {
    "reference": "MKLKDITLFKELATDAAGEVDISGVTSDSRKVERGFLFAALKGVKADGSAYAIDAVKRGAVAIIAGKNTALDNLGVPVLHVDDPRNALAVAAAQFYGKQPEVMVAVTGTSGKTSVASFTRQIWAYAGFPAANIGTTGVFSPTRSDYNSLTTPDPVELHRVLAELADEGVTHAAMEASSHGLDQRRLDGVKLAAAAFTNLGRDHMDYHSTIEEYLNAKMRLFDTLLPNGAPAIIFADDQFSPQAIEAAKAAGCDVKTVGRKGDFITIKRVEHERFRQHVEVRIGDEIFEVELPLAGDFQVANALVSAALAMVTGVPAAAAMRALERLKGAPGRLDLVGATEGGAPAYVDYAHKPEALENVLTSVRPFTTGRVIVVFGCGGDRDKGKRPIMGEIAARLADVAIVTDDNPRSEVPAQIRSEIMVAAQGAVEIGDRREAIFTAVSMMMPGDTLVVAGKGHEEGQIVGDITLPFSDHSEVAAALAARLEENKY",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.2. A protein should be able to manipulate the activity of enzymes in the peptidoglycan biosynthesis pathway, either by activating or inhibiting their function.3. A protein that can perform ATP binding in cytoplasm to support cell division.4. The protein should have a high affinity for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate. The designed protein sequence is ",
    "response#1": "MNVTGTKGKSTTTALLAHLLRAAGHCTALAGNIGLPLLELLTPQPAPEYWAIELSSYQTADVAGSGARPALALVLNVFPEHLDWHGGEQRYINDKLSLVTVARPPIALLNAADPRLASLVLPQSDLRWFNQRDGWHVRGQVVYRGEQAVLDTALIPLPGAHNGSNVCAVLALVEEVGFDRETILQALTEFGGVKHRLEPICTWSGIQFINDSKATNYDAAETGLVAVPAPVILIAGGQAKQGDDRRWLEMIQTKAAAVLLIGAAANLFAQRLQEVGYDRYEIVETMANAVPRAAELAKKYSANIVLLSPACASFDMFESYEHRAEVFRQAVESLS",
    "response#2": "ITGTNGKTTTTHVIHKIMSDHQVETAIIGTMYMKYKGKEIAVANTTPESLVLQKEYAHMVEAGVEAVTMEVSSHALELGRVHGTEFNIGVFTNLSQDHLDYHKTMEKYAQAKGLLFAQLGSSFSASNQNVAVLNLDDPYFSQLETMTAVPVLTYGMGEQADFRAVDVKIHENGADFTLQLGSEQYDIHLQMTGRFSVYNALAAAAVGLLLGYPLECITAALERFSGVPGRMERFVFDNGLCAVVDYAHSPDALENLLRSLRELCKGKLISVFGLGGERFRDNRWTMGEIAAKMADHLVLTMDNPRGEDPEDIVEDILTGVRKIPDASFEIVIEREKAVQIALDLGGAGDIVAISGKGHETYQIIGTTKHPFDDREWARLWLSQL",
    "response#3": "MKEVTGIAYDSRRVTPGMVFVAIAGQKTDGHGFISTAVDRGATAIICEKNGFVSQRVTQIKVADAREALAWAAAAYYGHPTRHLSMIGVTGTNGKTTCCFLITQTLQKLNCKSALMGTLGYGMADKVLQTQNLTTPDAVTLHQNYAALLEEGAKVIAMEVSSHGLDQNRLNGLKIDSGIFTNLTQDHLDYHKDMNTYLKAKQKLFERPELKRAIINLDSPYFDKVLAKINPKLPIFIYSLNQPKLLRRSYPVSTSFIYAKHYSFHNNGIVAEVVTPWGEGELRSPLMGDFNLSNLLAVYGTAIELGFDKEAIIQGLESAKGVAGRMERFVFDNGLCAVVDYAHSPDALENLLRSLRELCKGKLISVFGLGGERFRDNRWTMGEIAAKMADHLVLTMDNPRGEDPEDIVEDILTGVRKIPDASFEIVIEREKAVQIALDLGGAGDIVAISGKGHEDYQIIGTEKIHFDDREQAREALELKL"
  },
  {
    "reference": "MMVPVRCFTCGTVVGEHWGEFKERAREGDEDPAEVLDELGVERACCRRMLVSHKDLVDIVSPYQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein can be targeted to cytoplasm and perform DNA binding.2. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "LSLYWQSYHTIIPIVDADDFCAHYRSLWEPGQGATRRPSALVDIVLALCMQYSAALMASGDIELMDLESNDTNASGHWLYQRCQRLLLKDQDTLSLATLQTYIHSAVYLMNASCLNASHRVLSIAIHTATSLGLPHSPEGHPRGPQHALRQRIWWTLFFLDSKMSFELGQPYLMPEYR",
    "response#2": "LSLYWQSYHTIIPIVDADDFCAHYRSLWEPGQGATRRPSALVDIVLALCMQYSAALMASGDIELMDLESNDTNASGHWLYQRCQRLLLKDQDTLSLATLQTYIHSAVYLMNASCLNASHRVLSIAIHTATSLGLPHSPEGHPRGPQHALRQRIWWTLFFLDSKMSFELGQPYLMPEYS",
    "response#3": "LSLYWQSYHTIIPIVDADDFCAHYRSLWEPGQGATRRPSALVDIVLALCMQYSAALMASGDIELMDLESNDTNASGHWLYQRCQRLLLKDQDTLSLATLQTYIHSAVYLMNASCLNASHRVLSIAIHTATSLGLPHSPEGHPRGPQHALRQRIWWTLFFLDSKMSFELGQPYLMPEYL"
  },
  {
    "reference": "MDQCPIPTLLEHGNFSQVLFNNVEKFYAPRGDIMCYYTLTEKFIPRRKDWIGIFKVGWKTTQEYYTFMWAPLPKDQNKDSATQQEIQFKAYYLPKDVERYQFCYVDEDGLVRGTSVPFQFCPDPDEDIMVVINKEKVEEMEQLSEELYQQNQELKDKYADLHEQLQRKQVALEATQRVNKTLEHKVEEKASWEKEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEKEKASWEEEKASWEKEKAPWEVEKAPWKEVKAYWWNDLHR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be designed to localize specifically to cytoskeleton, autophagosome in order to perform its role in positive regulation of autophagosome maturation, response to type II interferon more effectively.2. The protein must exhibit the following characteristics: Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens upon entry into the cytosol by targeting LGALS8-associated bacteria for autophagy . The designed protein sequence is ",
    "response#1": "IPVSIFVTGDSGNGMSSFINALRIIGHEEDASAPTGVVRTTQTRAEYSSSHFPNVVLWDLPGLGATAQTVENYIEEMKFSTCDLFIIVASEQFSSNHVKLAKIIQSMGKRFYVIWTKLDRDLSTSVLSEVRLIQNIQENIRENLQKEGVKEVPIFLVSNLDPLLHDFPELRNTLQTC",
    "response#2": "IQNFYAKAAVLVLDSRIKSRPARGANGARKPNKWFQIETDEIDDFRDELKIWKNCGSLDNRPPPMVIEVYLDASRLKDSQSLVIVDENGKRWDVMEQLNSYGSSTDSSGASRRNNEVVIERWQVELKHSGMTSVDFGPILPTVYKKAIVFFRSLFITTRLLPAWKFASQGAAKNSHPALIPRCRIRLSQPDRPRYDQLRLPIDGRPDPVTEYVFGDLEVPVGRLSTLVTYRSDCNV",
    "response#3": "IQNFYAKAAVLVLDSRIKSRPARGANGARKPNKWFQIETDEIDDFRDELKIWKNCGSLDNRPPPMVIEVYLDASRLKDSQSLVIVDENGKRWDVMEQLNSYGSSTDSSGASRRNNEVVIERWQVELKHSGMTSVDFGPILPTVYKKAIVFFRSLFITTRLLPAWKFASQGAAKNSHPALIPRCRIRLSQPDRPRYDQLRLPIDGRPDPVTEYVFGDLEVPVGRLSTLVTYRSDCNV"
  },
  {
    "reference": "MKVKIISVGKVKDKGLNELINYYAKQIKTIEMVEITDEASITGMEKEGQKILSKIAKDSYVISLAIEGKMLDSVEFADTLDHVTTNFGPSITFIIGGSFGLSHAVKEKSNLLLSFSKMTFPHQLMKLFLVEQIFRAQAILNNHPYHK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. We are looking for a protein with broad substrate specificity for pseudouridine(1915) in 23S rRNA + S-adenosyl-L-methionine =   H(+) + N(3)-methylpseudouridine(1915) in 23S rRNA + S-adenosyl-L-   homocysteine.2. The designed protein should be able to compete with endogenous proteins or inhibitors for S-adenosyl-L-methionine binding.3. The protein must exhibit the following characteristics: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA..4. The protein must have high specificity towards rRNA (pseudouridine-N3-)-methyltransferase activity. The designed protein sequence is ",
    "response#1": "MFLIVGLGNPGTKXDI",
    "response#2": "MNKILVASRNAKKLSEIRAILAGTDLPGVELVSLDDVPGLGEVVEDGATFAENALKKARETAQASGLPAIADDSGLAVDILGGSPGIFSARWSGRHGDDAANLALLLAQISDIPDGQRGAAFVCAAALALPDGTERVVEGRLRGRLLREPRGDGGFGYDPIFIPDGREQTTAELAPEEKNAISHRGQAFRALAPVLRDLLG",
    "response#3": "MKILVLAGGK"
  },
  {
    "reference": "MVVDDLHRLASSQFKLPVAPILFTALAATIGAFLLSSLRSYILYHRKMSLFPAPNSDSAFRSFFSSKARDKFLSNAQELIRQGFSQNKFAIRLKTDFTDVLLLSPRYLPQLRTEDRLQGGPYTVQELLGYLPGFEPFRFADQMPKFIPDVILTRMNRYLSNVPASITEEVEVNLAENWTDEKDWHSVHLHGTMLGLVGQCSIRTFLGPEMCRKKRWVDLHTEYTVAVVTAVQALRKWPRALVSIVQWFHPKAKAARALLNEARAMIQPMHEQRKRDMAAGKPVPADTLTWFEEVAKGQAYDAAVVQLTMALAGLHSSTDLLCAVMLNLSEHPDVVEALRQELVQVLKREGWKQTTFSQLTLMDSVLKESQRLKPVGRAFFKRVAVDNIKLDHGVEIPKGAFVAVSNHGMWDPHNYTDPDKFDAYRFARMSDNKSSAFSTVSVEHTGFGFGKNSCPGRNYVALQLKIILAHLLLKYEWRLPDNYTPATFNNGFDLIADPFAQVLVRRRLEAPEVSLRQNT",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Ideal protein should have a binding affinity for key enzymes in the Mycotoxin biosynthesis pathway to enhance the overall efficiency of the pathway.2. The protein must be able to heme binding, oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen.3. The protein design should incorporate a binding pocket that specifically recognizes heme to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "MXFSSGKRICLG",
    "response#2": "MGQ",
    "response#3": "GFRS"
  },
  {
    "reference": "MAKLPVEKMRELERRFGEIEARMSAGPSADVYVKLASEYSELQPVVSKIRAYEKATAELADIGAMLADRATDKDMRDLAELEKPEIEERVEALEQEIQILLLPKDAADEKSAILEIRAGTGGSEAALFAGDLFRMYERYAAGKGWRVEVLSASEGEAGGYKEIIATVSGRGVFSRLKFESGVHRVQRVPETEAGGRIHTSAATVAVLPEAEDIDIDIRSEDIRVDTMRSSGAGGQHVNTTDSAVRITHLPTGLVVTSSEKSQHQNRAKAMQVLRSRLYDMERQRADSERSADRRNQVGSGDRSERIRTYNFPQGRLTDHRINLTIYKLDRVMEGEIDEIVDALLADYQASQLALLGEKQN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should exhibit Basic and acidic residues compositional bias.2. For general function, the protein need meet that Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.3. The protein should have translation release factor activity, codon specific and be active in cytoplasm. The designed protein sequence is ",
    "response#1": "QRLGELDFLLSREDIMSDMAQYRTISREHAEITQIAGRYERYKQREADIAGAAEMLEDPDMAEMAREEIAAAQAELVQLEEELQRLLLPKDPDDARNAFLEIRAGTGGDESALFAGDLLRMYTRYCERAGWRCEVVSESESELGGYKEVVIRIAGNDVFGRLRFESGGHRVQRVPATETQGRIHTSACTLLL",
    "response#2": "QRLGELDFLLSREDIMSDMAQYRTISREHAEITQIAGRYERYKQREADIAGAAEMLEDPDMAEMAREEIAAAQAELVQLEEELQRLLLPKDPDDARNAFLEIRAGTGGDESALFAGDLLRMYTRYCERAGWRCEVVSESESELGGYKEVVIRIAGNDVFGRLRFESGGHRVQRVPATETQGRIHTSACTIL",
    "response#3": "QRLGELDFLLSREDIMSDMAQYRTISREHAEITQIAGRYERYKQREADIAGAAEMLEDPDMAEMAREEIAAAQAELVQLEEELQRLLLPKDPDDARNAFLEIRAGTGGDESALFAGDLLRMYTRYCERAGWRCEVVSESESELGGYKEVVIRIAGNDVFGRLRFESGGHRVQRVPATETQGRIHTSACTL"
  },
  {
    "reference": "MKLLVAAVGRWRGGPERALFDHYVGRLKGGLAVIEVEERRPLPVAERKAREAELLLAQLPPGAFVVALDEHGEAWGSERLARELDQRRLEARPAAVFAIGGADGHGEALLARADRKLALGVMTWPHMLVRGLIAEQLYRAQCISGGHPYHRS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA.2. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining rRNA (pseudouridine-N3-)-methyltransferase activity. The designed protein sequence is ",
    "response#1": "MENTKI",
    "response#2": "MXVFXW",
    "response#3": "MSPTLAV"
  },
  {
    "reference": "MNWKSLACCVSAAALFASTGCSTQSGAAMAEADDKTAEAPLLADQTSEAKSEKTNNPDHEQHKVAVMKPKEIAACPVMGNVNPPSARNTAAGVMSNGDWWPNQLNLKILHQNSAKGNPMGDEFDYAKEFEQLDLAAVKADIKQLLTDSQDWWPADYGHYGPLFIRMAWHSAGTYRVGDGRGGSGYGTQRFAPLNSWPDNASLDKARRLLWPIKQKYGDKISWADLMILTGNVSLESMGFETFGFGGGRADVWEPQEDIYWGPESEWLGDKRYTGDHHLENPLAAVQMGLIYVNPEGPNGKPDPIAAAHDIRDTFGRMGMNDEETVALIAGGHTFGKAHGAGDAKNVGPAPEGATIEEQGLGWKNSFGTGKGADAITSGLEGAWSTTPTEWSNGYFDNLFGYEWVLTKSPAGAHQWTPKDEAAQGTVPDAHDPLKTHAPMMFTTDMALKMDPAYGEISKRFHENPDQFKQAFSKAWYKLTHRDMGPVTRLLGPEVAEPQIWQDPVPKADYKQINDADVAKLKTKLLDSGLSTGELVSTAWASAATFRGSDKRGGANGARIRLAPQKDWTVNQPEKLAKTLAVLEQVQKEFNDAQTDGKQVSLADVIVLGGCAAVEDAAKKGGFDVKVPFSPGRTDATQEMTDVESFSYLEPKSDGFRNHINAEIDRPAEELLVNRAQLLTLTAPEMTALVGGMRVLDTNFGDGPLAELGVFTDKPGTLSNDFFVNLLTMDTAWEKSAVCDHFFEGKDRKSGEVKWTATRVDLIFGSNSQ",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The heme b binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must be engineered to have a cooperative binding mechanism with heme b to ensure proper regulation.3. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for AH2 + H2O2 = A + 2 H2O.4. The designed protein should contain one or more Plant heme peroxidase family profile domains that are essential for its function.5. A protein with metal ion binding, heme binding, catalase activity functions is required to enable response to oxidative stress, hydrogen peroxide catabolic process.6. The protein must exhibit the following characteristics: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity..7. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "AKAWFKLTHRDMGPLSKYIWEKEPKETLIWQDPIPSVDHKLIGEKEIKSLKGKILGSGLTIPELVRTAWAAAASFRSTDMRGGANGGRLRLSPQREWTVNNPDELKKVLKELTEIQREFNESGKKVSLADVIVLGGVAAVEEAAKKAGVKVSVPFTPGRMDASQESTDVKSFAVLEPKADAFRNYYGDGNYLSPTEALVDRANMLSLTVPEMTVLVGGLRALNANSANSKHGVLTQRPGVLSNDFFVNLLDMSTKWQKSSDNAAVYEGIDRKTGAKKWSATSVDLIFGSHSELRSVAEVYASPEVQE",
    "response#2": "AKAWFKLTHRDMGPLSKYIWEKEPKETLIWQDPIPSVDHKLIGEKEIKSLKGKILGSGLTIPELVRTAWAAAASFRSTDMRGGANGGRLRLSPQREWTVNNPDELKKVLKELTEIQREFNESGKKVSLADVIVLGGVAAVEEAAKKAGVKVSVPFTPGRMDASQESTDVKSFAVLEPKADAFRNYYGDGNYLSPTEALVDRANMLSLTVPEMTVLVGGLRALNANSANSKHGVLTQRPGVLSNDFFVNLLDMSTKWQKSSDNAAVYEGIDRKTGAKKWSATSVDLIFGSHSELRSVAEVYASKC",
    "response#3": "WWPADYGHYGPLFIRMAWHSAGTYRTVDGRGGAAGGRQRLPPISSWPDNVNLDKARRLLWSVKQKYGRKLSWGDLIVLAGNVALESMGFETFGFAGGREDDYVPDKAVDWGPEDEWEKTSSDRFDEEGDLKEPLGNTVMGLIYVNPEGPNGEPDVEGSAKNIRQAFSRMAMNDEETVALIAGGHTFGKVHGADSGDNLGPEPEAAPIDLQGLGWENEYGSGKGPDTITSGIEGPWNTTPTQWDMGYIDNLLEYKWWPEQGPGGAWQWTTQGGELDDAAPGVEDPSEKEDVMMLTTDIALKRDPDFREVLERFQED"
  },
  {
    "reference": "MAVKPTKAEKKIAYDQKLCQLLDEYSQVLIASADNVGSNQLQAIRIGLRGDSIVLMGKNTMMKRSIRLHAENTGNENLRNVEQLFLPNVGLIFTKGDLNQVREEISKYKVGAPARFGLVAPIDVVVPPGNTGLDPSQTSFFQVLNIPTKINKGTVEIITAVELIKKGEKVGSSEAALLAKLGIRPFSYGLNVESVYDDGSVFSPEVLDLTEDDLLARFATGVSMVTSLSLAISYPTLAAAPHSSSMGATMFLLLLLQPTMTSLRPRKLRSISRILAVASCSCSSAGTAPTGGGAAAAAVEEKKEEPEEESDDDIGFSLFDD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein's functionality will benefit from Acidic residues compositional bias in its structure.2. The protein should have a specific function in ribosome biogenesis and should be localized to ribonucleoprotein complex in order to carry out this function efficiently.3. The protein must exhibit the following characteristics: Ribosomal protein P0 is the functional equivalent of E.coli protein L10.. The designed protein sequence is ",
    "response#1": "GTELGNAMYIMNIPLGTIISCVELIPGKGAILARSAGSYAQLIAKSKKFATIKLPSGEKRMVLSTCMATIGSIYNSDHKLEMDGKAGRKRWLGRKPRTRGVAMNPVDHPMGGGEGKSSGGHPRNRNGIG",
    "response#2": "MPTELGNAMYIMNIPLGTIISCVELIPGKGAILARSAGSYAQLIAKSKKFATIKLPSGEKRMVLSTCMATIGSIYNSDHKLEMDGKAGRKRWLGRKPRTRGVAMNPVDHPMGGGEGKSSGGHPRNRNGIP",
    "response#3": "LGNAMYIMNIPLGTIISCVELIPGKGAILARSAGSYAQLIAKSKKFATIKLPSGEKRMVLSTCMATIGSIYNSDHKLEMDGKAGRKRWLGRKPRTRGVAMNPVDHPMGGGEGKSSGGHPRR"
  },
  {
    "reference": "MNKKSVLVIHGGAGVISKSTISKEREEIFLNSLKNILLAGKIILKQGGTSLDVVQEAVRLLEEDPIYNAGKGSVFTELGTNEMDAAIMDGTNLKAGAVGGVSIIRNPIIAARAVMEHTNHCLLVGKGAEEFAKSKNLEIVEPSFFFTQNRYDQLLRAKDEKKLILDHDGENLLEKEKEKEKNNETSTTTTTISVGVDPIDPKYKMGTVGAVCLDSFGNLAAATSTGGMTNKMHGRVGDTPIIGAGVYANKNVAVSSTGTGEAFMRTVAAFDIAAMMEYGSLSLKDASNKVVMEKLITVGDGGVICVDKYGNVEMPFNTEGMYRGYVIIDNNCENDQNDIINVSIYK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a specific function within the proteasome core complex. The designed protein sequence is ",
    "response#1": "DFKTAFSYFYEAFEGYDSVDEKVSALTALKYMLLCKVMLDLPDEVNSLLSAKLALKYNGSDLDAMKAIAAAAQKRSLKDFQVAFGSFPQELQMDPVVRKHFHSLSERMLEKDLCRIIEPYSFVQIEHVAQQIGIDRSKVEKKLSQMILDQKLSGSLDQGEGMLIVFEIAK",
    "response#2": "EIMAPPGMEEMTNQLQGLFQNLAGDTKKKRKMKIKDALKALVEEEAAKLVNQEELKEQAIYNVENNGIVFIDEIDKICKRGEVSGPDVSREGVQRDLLPLIEGSTVSTKHGMVRTDHILFIASGAFQVAKPSDLIPELQGRLPIL",
    "response#3": "VFEKLDNGYIHIEDGNEYLTDFLSFISPQYLYKKHLNIIKSKGHVRPSEISIIRNLCMSSDCFDIIKKNITAEDILAMPYLEQMILLEKMSQYDYSVKFLAQHLPKVMGSLISKDVKELLDRETFDFRLQVINNLIEWDVEQLHIWYEPLTEAKAQCMGTFASKDVGTKIESSC"
  },
  {
    "reference": "MSRKSIDYGALPEYEKSQIKRTLELGTVMTVFSLKKFNPERRTIQVIMETRQVAWSKTADKIEGVLDLMEIKEIRSGKNSKDFERGKATRHKDDHCFTIFYGTQFVLCTLSLAADSKGDAEKWLCCLSILHQEAMSAPTPAVTESYSCMFFSITLRELKTLLPLVNFKVSGVKYLKDKFMEIGAHKEELSFEQFHLFYKKIMNTDRPNASVVHLHDFQRFLHHEQQESWAQDLSKVRERMTKFIDDTMRETAEPFLYVDEFLAYLFSKENSIWDDKYDATDPQDMNNPLSHYWISSSHNTYLTGDQLRSESSTEAYVRCLRMGCRCIELDCWDGPDGKPIIYHGWTRTTKIKFDDVVQAIKEHAFVASEFPVILSIEEHCSVEQQRHMAKVFKEVFGDQLLIKPIEASADQLPSPTQLKEKFIIKHKKLGPKGDVDVNTEDKKEEKQQQGELYMWDTIDLKWTRHYCAVADEKLSFSDDIEQNADEDQSKEVKQTELHCGEKWFHGKMKEGRTTAEKLLQEYCAEMGGKDGTFLVRESETFPNDFTLSFCRSGRVQHCRIRSFADGDAIKYYLTDNLPFDSIYDLIQHYREAHLRCAEFELRLTDAMPNPKPHETKDWYYSNLSRGEAEEKLMRIPRDGAFLIRKRDEPSSYAMTFRAEGKIRHCRIKQEGRHFVLGTSAYFESLVELVNYYEKHPLFRKMKLRYPVTEELLSRYSTASDSLFNLAHVYLELCNFTNVSPSLPVFSVPLPVLDCKPLGSRDLSITIDC",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Plays an important role in the regulation of intracellular signaling cascades..2. The protein must exhibit scaffold protein binding, phosphotyrosine residue binding, protein tyrosine kinase binding. The designed protein sequence is ",
    "response#1": "DKECFKCALGSSWIISVELAIGPEEGISYLTDKGSNPTHLADFTQVQTIQYSSSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVSGASESFIIRPQL",
    "response#2": "DKECFKCALGSSWIISVELAIGPEEGISYLTDKGSNPTHLADFTQVQTIQYSSSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVSGASESFIIRPQC",
    "response#3": "RGAPHTSDQVLGPGVTYVVKYLGCIEVLRSMRSLDFSTRTQVTREAISRVCEAVPGAKGALKKRKPPSKMLSSILGKSNLQFAGMSISLTISTASLNLRTPDSKQIIANHHMRSISFASGGDPDTTDYVAYVAKDPVNRRACHILECCDGLAQDVIGSIGQAFELRFKQYLG"
  },
  {
    "reference": "MKILFCLLLLAITALEAGVVKRYNKRFAGFNVAGIGGNAGCVVVDNKLFANSFFLRELTTEEQRELAQYIEDSNRYKEEVKESLEERRKGWQLARDGKEDSKVLSALAEKKLPKPPKKPSFCSAGDTTQYYFDGCMVQNDKIYVGRAYVRDLTPDEVTQLKTFDAKMTAYQKYLSSTIQKQVDSLFGEKSNLFNLFADTRTEATSQASDDATAGATTTQAPVEAPEPPHFCVAIY",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a specific function in negative regulation of peptidase activity and should be localized to extracellular region in order to carry out this function efficiently.2. The protein must exhibit the following characteristics: Aspartyl protease inhibitor.. The designed protein sequence is ",
    "response#1": "KPPKKPSFCSAGDTTQYYFDGCMVQNNKIYVGRMYVRDLTSDEINQLKTFDAKMTAYQKYLSSSIQQQVDSLFN",
    "response#2": "KPPKKPSFCSAGDTTQYYFDGCMVQNNKIYVGRMYVRDLTSDEINQLKTFDAKMTAYQKYLSSSIQQQVDSLFS",
    "response#3": "KPPKKPSFCSAGDTTQYYFDGCMVQNNKIYVGRMYVRDLTSDEINQLKTFDAKMTAYQKYLSSSIQQQVDSLFD"
  },
  {
    "reference": "MQNKVYEQEDKMEKQEQREDKLGTPPVYSLVHFSPKMVGSVATTLPLSSSLSSGMPLGYHLSSPQISGVTISTTGQLTSKATAGSCSRMENSLDAGPFLVPKKQDESPMIGDGEDYFLSLFGDSKKLIAHSKHTQKTLKYFSMILEEVGQFTASSLGDIEIAEVNVKGLFVKLINSSLDKEMAIGDHILQQNVNGQTISLYRFLPNIVMQASSTVTVWAAASEAKHQPPSDFLWKEQDKFRTSPDCITILCKPNGQAIAWYTPIHWKQAWEKLDTDIEFNRCSVVSPTFRKHVFQWTASTTTITKEKQDQPKEDISNYQVEQAQVFLKREKEIPPTVFPNRSPWCQNPYVSAHPYCPLIEPRNTSTAGGNLDRQSRSQPTRPNRASEKFTKSLNASKKMSYLWQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should exhibit Basic and acidic residues, Polar residues compositional bias.2. The presence of LTD domains is critical for the interaction of the designed protein with its intended targets.3. The designed protein should have a specific function within the intermediate filament. The designed protein sequence is ",
    "response#1": "EGLEETLRNLQARYEEEMLSREDAERLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVSTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETR",
    "response#2": "ELSQLLNEIRANYEKILTRNQIETVLSTRIQLEEDISKKMDKDEEALKAAQAELKEARRQWHHLQVEIESLHAVERGLENSLHASEQHYQMQLQDLETVIEGLEKELQEVRRGIEKQLQEHEMLLNTKMRLEQEIATYRHLLEKEEIL",
    "response#3": "QEREQIKTLNNKFASFIDKVRFLEQQNKVLETKWALLQEQGQNLGVTRNNLEPLFEAYLGSMRSTLDRLQSERGRLDSELRNVQDLVEDFKNKYEDEINKHTAAENEFVVLKKDVDAAYMGRMDLHGKVGTLTQEIDFLQQLYEMELSQVQTHVSNTNVVLSMDNNRNLDLDSIIAEVKAQYELIAQRSRAEAEAWYQTKYEELQVTAGKHGDNLRDTKNEIAELTRTIQRLQGEADAAKKQCQQLQTAIAEAEQRGELALKDAQKKLGDLDVALHQAKEDLTRLLRDYQELMNVKLALDVEIATYRKLLESEESRM"
  },
  {
    "reference": "MSVLEKPKKTAEQDWHRADILAELKKNGWSLRSLAKEGQVSYNTLKTVLDKSYPKMERLVANAIGVPPEVIWAGRFAERNKRPTLQHKY",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Also negatively regulates its own gene transcription ..2. The H-T-H motif domain within the designed protein should be capable of recognizing and binding to a range of DNA sequences. The designed protein sequence is ",
    "response#1": "HMGSLRVACYTAPALSFMSGVIQTFIADRPDVSVYLDTVPSQTVLELVSLQHYDLGISILAGDYPGLTTEPVPSFRAVCLLPPGHRLEDKETVHATDLEGESLICLSPVSLLRMQTDAALDSCGVHCNRRIESSLALNLCDLVSRGMGVGIVDPFTADYYSANPVIQRSFDPVVPYHFAIVLPTDSPPPRLVSEFRAALLDAL",
    "response#2": "HMGSLRVACYTAPALSFMSGVIQTFIADRPDVSVYLDTVPSQTVLELVSLQHYDLGISILAGDYPGLTTEPVPSFRAVCLLPPGHRLEDKETVHATDLEGESLICLSPVSLLRMQTDAALDSCGVHCNRRIESSLALNLCDLVSRGMGVGIVDPFTADYYSANPVIQRSFDPVVPYHFAIVLPTDSPPPRLVSEFRAALLDAL",
    "response#3": "HMGSLRVACYTAPALSFMSGVIQTFIADRPDVSVYLDTVPSQTVLELVSLQHYDLGISILAGDYPGLTTEPVPSFRAVCLLPPGHRLEDKETVHATDLEGESLICLSPVSLLRMQTDAALDSCGVHCNRRIESSLALNLCDLVSRGMGVGIVDPFTADYYSANPVIQRSFDPVVPYHFAIVLPTDSPPPRLVSEFRAALLDAL"
  },
  {
    "reference": "MFTPKCEKGSFVPFLESHRDDERIENSLLQKSSCKIGSFTLKNESQSITDHYEHFLPLLEITTTTKINKDLSGLQGDRKPVTRAISTTSRVHLGRKLESGVEPDETLFALPAASEPVIHSSAEQIMDLKEKPGNTVSAGQEDFPSVLLETAASLPSLSPLSAASFKEHEYLGNVPAVLSTEGTLQETLNEASKQLSEKAKNPFVDRDLTEFSELEYSEMGSKFSGSPKAESAIMVANPREEVIAKNKDEEEDLVSNNILRNQQGLPVALTELVKEEDKVMFPEKTMDSFNEKEAAVEAPIREEYADFKPFERVWEVKDAYKEDSGMPAAGGSIESKLESKADEKYFADSLEQTNHEKESESSNDDTSFPSTPEAVKDGSGAYITCAPFNPAATESIATNIFPLLEDHTSENKTDEKKIEEKKAQIVTEKNISTKSSNPFLAATQDSQTEYVTTENLSKVTGEAVANMPEGLTPDLVQEACGSELNEAAGTKIAYETKMDLVQTSEAMQESLYPVTQLCPSFEESEATPSPVLPDIVMEAPLNTVVPSAGASAGQPSSSPLEAPPSANYESVKLDSESPPPYEEAMNVKKISGIKEEIKEPETTDAVVQETEAPYISIACDLIKETKLSSEPSPDFANYSEIAKVEQPVPDRSELVEDSSPDSEPVDLFSDDSIPEVPQRQDEAVILLKENLTETSYESALEYENKEKLSTLLPEGGKPYLESFQSNLSTTRDSPSPDEVLTLTKKEKIPLQMEDLKTAVYAKEDLLVS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein's functionality will benefit from Basic and acidic residues, Polar residues compositional bias in its structure.2. The designed protein should have ubiquitin protein ligase binding within cell projection, endoplasmic reticulum tubular network membrane, postsynaptic density, nuclear envelope, glutamatergic synapse to regulate positive regulation of epithelial cell migration, cellular sphingolipid homeostasis, cerebral cortex radial glia-guided migration, endoplasmic reticulum tubular network membrane organization.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.4. The designed protein must have at least one Reticulon domain. The designed protein sequence is ",
    "response#1": "YTSVIYFSCDKFIRNAHPEFAGLGADSCTYQFNFATPLACNDLKPCTAFTSTNELLDLSSLSSKPARTLLKDGKNYTIAVCAHAGAPCQENGGACYEQNSTTISLGNSNSQLRFNQTGSLYLLYEDGAEC",
    "response#2": "YTSVIYFSCDKFIRNAHPEFAGLGADSCTYQFNFATPLACNDLKPCTAFTSTNELLDLSSLSSKPARTLLKDGKNYTIAVCAHAGAPCQENGGACYEQNSTTISLGNSNSQLRFNQTGSLYLLYEDGAEC",
    "response#3": "YTSVIYFSCDKFIRNAHPEFAGLGADSCTYQFNFATPLACNDLKPCTAFTSTNELLDLSSLSSKPARTLLKDGKNYTIAVCAHAGAPCQENGGACYEQNSTTISLGNSNSQLRFNQTGSLYLLYEDGAEC"
  },
  {
    "reference": "MRVKGMKRNCQHLLTWGTIILGLIIMCSTADNLWVTVYYGVPVWRDAETTLFCASDAKAYKTEAHNVWATHACVPTDPNPQEIELKNVTEKFNIWKNXMVEQMHTDIISIWDQSLKPCVKLTPLCVTLNCTNVXNVTNVNASVTAKEEMKNCSFNITTEIRDKKQKVXSLFYKLDIVPIDKNNTSNNYSEYILINCNTSAITQACPKVSFEPIPIHYCAPAGFALLKCNDKEFNGTGLCRNVSSVQCTHGIKPVVSTQLLLNGSLAEGNIVIRSENISNNAKTIIVQLNQSVXINCTRPSNNTRKSIRIGPGQAFYATGEIIGDIRQAHCNVSRSDWNNTLXQVANKLRTYWNKTIXFTKPSGGDLEITTHSFNCGGEFFYCNTTXLFNSTWTGNETXXTDNXXDNITLPCRIKQIINVWQRVGQAIYAPPIQGQIECKSNITGLLLTRDGGSSNESTHNETFRPGGGDMKDNWRSELYKYKVVKIEPLGVAPTKAKRRVVEREKRAIGLGAMFIGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQSNLLQAIEAQQHLLKLTVWGIKQLXARVLAVENYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNKTYDDIWGNMTWLQWDREIENYTGTIYNLIEAAQNQQEKNEQDLLALDKWASLWNWFDIXXWLWYIKIFIMIVGGLIGLRIVFAVLSIINRVRQGYSPLSFQTHLPSPRDPDRPEXIXEEGGGQDRDRSIRLVNGFLAIVWDDLRNLCLFSYHRLRD",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should emphasize Basic and acidic residues compositional bias.2. For general function, the protein need meet that Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain3. The protein design must be focused on enhancing the efficiency of virion attachment to host cell, viral protein processing. The designed protein sequence is ",
    "response#1": "MGAASLTLTVQARLLLSGIVQQQNNLLRALEAQQHLLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICSTNVPWNASWSNKSLDHIWGNMTWMQWEKEIDNYTDLIYTLLQESQNQQEKNEQELLELDKWGNLWNWFSITNWLWYIKIFIIIVGGLIGLRIVFAVLSIVNRVRQGYSPLSFQTTR",
    "response#2": "MGAASLTLTVQARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLRDQQLLGIWGCSGKLICTTTVPWNSSWSNKSYNEIWDNMTWMQWDREINNYTATIYKLLEDSQIQQEKNEKDLLALDSWKNLWNWFDISNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTR",
    "response#3": "MGAASLTLTVQARLLLSGIVQQQNNLLRALEAQQHLLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICSTNVPWNASWSNKSLDHIWGNMTWMQWEKEIDNYTDLIYTLLQESQNQQEKNEQELLELDKWGNLWNWFSITNWLWYIKIFIIIVGGLIGLRIVFAVLSIVNRVRQGYSPLSFQTGR"
  },
  {
    "reference": "MARTPTRLLIAASGTGGHLFPALALAERLPDYEIEWLGVPDRLEQSLVPKTYPLHTIPIEGFQTRLGLKTLKILFSQLRAIWQVRSLIKKRQIAAVFTTGGYIAGPTILAARLANIPVILHESNYIPGKVTKVLGRWCDTVALGFQGTASYLPGTRTTWVSTPVREQFLIPQSLDLPIPETAFLIVVAGGSQGAVALNQLVRQSAPQWLEKGIYIVHLTGENDPEADSLRHSNYFSQPFYDNMAGLWQRANLAISRSGASTLTELAITCTPSILIPYPFAAEDHQFYNAQVFAEAQAAYLYRQNELTAQFLSELVLDLWSNPEKLAAMAQQASHLAISDSADLLADLLRRKSQKDSF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II).; Cell wall formation2. The desired protein should be able to perform di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-   alanine + UDP-N-acetyl-alpha-D-glucosamine = di-trans-octa-cis-   undecaprenyl diphospho-[N-acetyl-alpha-D-glucosaminyl-(1->4)]-N-   acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-   D-alanyl-D-alanine + H(+) + UDP The designed protein sequence is ",
    "response#1": "MKLILLTGGGTGGHLAIAKALMESLASKNQSLFFIGSQRGQDRSWFEHEEGFKRRFFLETQGVVNQRGIAKIRSLSSQFQAMLEARKILKNHQIKRVVSVGGYSAAPASLAALSLGIPLYIHEQNAKVGLLNRLLKPFEFLLQHPQCRHIFTGVLVVGGSQGARVMSDIVPGAIEKLGSPLWQRLVLTQQVRDEDMARVRAVYERLRINAELAPFFADLPSRLASSHIIVSRSGAGTVAELGAIGRPSILVPLPGAIDQDQFANAGVLADVGGAIRIVQSDFTPDRLAAELSALAADPARLAAMAAAARTVGRLDAAERLIAGGGTGGHLFPGIAVAEEFLSRKKGNEVLFVGTWRGIEARVLPKLGYRLECITAAGIRGKGSVARAKGLAKFLYGYAQSRKILKEFKPDLVLGVGGYASAPALLAARGMHIPRFIHEQNAIPGFTNKMLATVAERVFPAAQGAAPVLVVGGSGGALKINRAVVEILNQYQPNKYRLLLVTGKRLYKSTLESINAESLQSKHKVFAYVNDMPHALKACDLIVCSAGAITIAEVTAVGKASILIPKAHTAENHQEYNANAMGNKGAAVVIREDELSGEILNKKIQDIIGNIQVVKKMEAASYKEGIRDAADRSYQAIAETIEVLIMAGGTGGHVFPALACAREFQTRGYAVHWLGTSRGIENELVPQAGLPLHLINVSGLRGKGKLSLLKAPFQLLRSLLQARRIVRELQPVCVLGMGGYVTGPGGLAARLAGVPLVIHEQNAVAGTANRLLSRIATRVLVVGGSGGALKINRAVVEILNQYQPNKYRLLLVTGKRLYKSTLESINAESLQSKHKVFAYVNDMPHALKACDLIVCSAGAITIAEVTAVGKASILIPKAHTAENHQEYNANAMGNKGAAVVIREDELSGEILNKKIQDIIGNIQVVKKMEAASYKEGIRDAADR",
    "response#2": "MRLILLAAGGTGGHLFPAEALGVELMKRDLRVRLVTDSRALRYSGLFSKDMTDVVPSETVRGRSPLALARTGLMLATGTVVALNLMRRLKPAAVIGFGGYPTLPPLIAARLKGIPTVIHDANAVMGRANRLLSRRVNAILCAFPEARAVFSGNPVRAEIAALPADAARERYALRADGPLVLLVFGGSQGAVSLNRAVAGAAEDLAASGVAVLHAYGLKNTLELRTPEYGEPPYVAVPYLDRMDLAYAAADLVICRSGAMTVAEVSAVGLPAIYVPFPIGNGEQRLNALPVVNAGGGLVVADADLTPGLVARQVVRLFSDPAQLAAMTAAAARVGHRDAAHRRVAEAAQWQAVARAVAEVAG",
    "response#3": "MSVLVTGGGTGGHIYPALAVINELKERNQIVDILYVGTSKGMEQEIIPNRGIDFAAITVRGLQRKINLEQVYFLRDFLKGLYQSYRLIKNFTPDVVIGTGGYVCGPVLMAASLMKIPTVLHEQNVIPGITNKFLSRFAVLVVGGSGGALKINRAVVEILNQYQPNKYRLLLVTGKRLYKSTLESINAESLQSKHKVFAYVNDMPHALKACDLIVCSAGAITIAEVTAVGKASILIPKAHTAENHQEYNANAMGNKGAAVVIREDELSGEILNKKIQDIIGNIQVVKKMEAASYKEGIRDAADRVIRELNDLLGGAQPKPAALPPADLDGHPTRTTDAAQTDAADTDAADTDAAGTDAAGTDAAGTDAAGADLVMAGGTGGHVFPGLAVARALRDEGWRVVWLGNRTGMEATLVPKHDIPMEYIQFGGLRGKGLLTKLLLPLNLLRAFWQSIGALRRVKPDVVLGMGGYITFPAGMMASLLGRPLVLHEQNSIAGLANKVLAKVADRVAHTFPELRTDPPVRLVGNPVRPEIAALPVQDRDRYRFADDAPLTLLVFGGSRGARSLNNAIASQLDTLLASVNLIWQTGSLDGEKLKAEVKPSPYLWMAPYIEDMEAAYSAADVVVCRAGASSLAELTNLGKVALLVPYPYATADHQRHNAQSLVEHGAALMVADSEAFTKLVPTALELLQNSGKRAAMSVAAAKQAHPDARAVADIASRGGGVTAAAEVVADIIEQV"
  },
  {
    "reference": "MVADVLLTHFNQLFCLNDPGHPLTGQEMKKATIVEDGYIAIKDGLIVALGSGEPDAELVGTQTIMRSYKGKIATPGIIDCHTHLVYGGSREHEFAKKLAGVSYLDILAQGGGILSTVRATRSASFDNLYQKSKRLLDYMLLHGVTTVEAKSGYGLDWETEKRQLDVVAALEKDHPIDLVSTFMAAHAIPEEYKGNPKAYLDVIIKDMLPVVKEENLAEFCDIFCEKNVFTADESRYLLSKAKEMGFKLRIHADEIASIGGVDVAAELSAVSAEHLMMITDDGIAKLIGAGVIGNLLPATTFSLMEDTYAPARKMIDAGMAITLSTDSNPGSCPTANMQFVMQLGCFMLRLTPIEVLNAVTINAAYSVNRQERVGSLTVGKEADIAIFDAPNIDYPFYFFATNLIHQVYKKGQLTVDRGRIL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein should be able to manipulate the activity of enzymes in the L-histidine degradation into L- glutamate pathway, either by activating or inhibiting their function.2. The protein should have a fast turnover rate for 4-imidazolone-5-propanoate + H2O = N-formimidoyl-L-glutamate.3. A protein that localizes to cytoplasm and can perform zinc ion binding with high specificity and efficiency.4. The protein must exhibit the following characteristics: It is the third step in the universal histidine degradation pathway.. The designed protein sequence is ",
    "response#1": "LVTPGLVDSHTHLVFAGERAGEFALRCAGRSYLQVALSGGGIAVTTRATRAAPDEQLLADAAARARRLIAQGVTTIEVKSGYGLDAPEELRLLRIVHRLGDALGGDATILPTLLFHAVPPEQVGDRAGFVREACASLIPQVARERLAGFCDVFVEDGAFAPDEARLLLQAAKDRGLVPRVHAEQLTAGGGARLAAELGCSSADHLEELDDAGVAALAEARVVAGLLPLSTLFLGSERYAPARRLLEAGVPVSLATNMNPGSAMSENVGLTLSLACLKLGLTPAEALVAFTAGGARALRQPDLGRIARGADADLVLWGCGSPEHLAWHMAVNHALAVVKHR",
    "response#2": "LVTPGLVDSHTHLVFAGERAGEFALRCAGRSYLQVALSGGGIAVTTRATRAAPDEQLLADAAARARRLIAQGVTTIEVKSGYGLDAPEELRLLRIVHRLGDALGGDATILPTLLFHAVPPEQVGDRAGFVREACASLIPQVARERLAGFCDVFVEDGAFAPDEARLLLQAAKDRGLVPRVHAEQLTAGGGARLAAELGCSSADHLEELDDAGVAALAEARVVAGLLPLSTLFLGSERYAPARRLLEAGVPVSLATNMNPGSAMSENVGLTLSLACLKLGLTPAEALVAFTAGGARALRQPDLGRIARGADADLVLWGCGSPEHLAWHMAVNHALAVVKHR",
    "response#3": "TVLPGFVDPHTHALFAGDRSDEFAAKLRGKPYQEILAEGGGILRTVDAVRAASDAALVANLTAQLDVMLAHGTTTAEVKTGYGLDTETECRMLDAIAAAAAEHPVDVVTTFLGAHAVPDDTDADAYVDAVIDDQLPAAANGPARFCDVFCEADVFTVEQSRRILDAGREHGLAPKLHAEEFTRLGGAQLAADLGATSADHLLHATPEDAAALADAGVTPVLLPATAFVLDEAYADPQQFLAAADNRTGAPVALGTDLNPNCYTHSMGFVVSLACNGMRMAPADAVLAATAWAASALDRGRDGTGTLREGTDGDVLVVDAPSHVHLPYNPGVNNVEAVLTDWITPGLVDCHTHLVYGGDRAHEFELRLAGASYEEIARAGGGIVSTVAATRAADENELVTGALPRLDALLAEGVTTIEIKSGYGLETATELRQLSAARALGVRRAVSVRTSFLGAHALPVEADGDKERYIDRVCDEMLPAVAQSGLADAVDAFMENIAFSAAQTSRVFAAAKALGLPVKLHADQLSNLGGAALAAEFGALSADHLEHTDEAGAAAMARAETVAVLLPGAFYFIRETQKPPVELFRKHGVKLALATDCNPGSSPLTSLLLTMNMAATLFRMTVDECLAGVTREGARALGVLAETGTLDAGKWCDLAIWDD"
  },
  {
    "reference": "MQIYYDKDADLSIIQGKKVAIIGYGSQGHAHANNLKDSGVQVVVGLRPGSASAKKAENAGLAVASVEDAVKQADVIMILAPDEHQARLYNEQIAPNIKQGAALAFAHGFNIHFEQITPRADLDVIMIAPKGPGHLVRSTYTQGGGVPSLIAVYQNASGRAKELALSYASANGGGRAGIIETTFREETETDLFGEQAVLCGGATALVQAGFETLVEAGYAPEMAYFECLHELKLIVDLMYEGGIANMRYSISNTAEYGDLTRGPRIVTEQTKQEMKKILREIQTGEFAREFILENQAGAATLKAKRRLGREHLIESVGARLRDMMPWIKANRIVDTSKN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a high specificity for substrate, NADP(+) binding, with minimal off-target effects.2. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.3. The protein must exhibit the following characteristics: In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.; Involved in the biosynthesis of branched-chain amino acids (BCAA).4. A protein that is capable of catalyzing reactions within the L-valine from pyruvate: step 2/4, L-valine biosynthesis pathway with high efficiency and specificity.5. The designed protein must be optimized for NADP binding, ketol-acid reductoisomerase activity, magnesium ion binding.6. The designed protein must have at least one KARI C-terminal knotted domain.7. The protein should be designed to catalyze the following reaction (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-   ethyl-2-hydroxy-3-oxobutanoate + H(+) + NADPH. The designed protein sequence is ",
    "response#1": "MKIYYDDDANLELIKKLNVTIIGYGSQGHAHANNLKDSGVDVTVGLREGSSSWSKASEAGLKVMPVDQSVLNADLVMILAPDEFQKGIFEKSIKPNLKSNAILAFAHGFNIHFKKIVPSETNTVIMIAPKGPGHTVRSTYLNGGGVPSLIAIFNDAMQEGEHSAKDIALSYAKANGGTRAGVLETNFREETETDLFGEQAVLCGGAVELVKMGFETLTEAGYAPEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGTEVINEQSRVAMRNALKRIQTGEYAKMFIQEGKTNYPSMTARRRLNAVHPIETVGGQLRDMMPWISLGKADKIHPLQEKAFKEETETDLFGEQAVLCGGLTALIKAGFETLVEAGYSPEMAYFECLHETKLITDLIQEGGIANMHYSISNTAEYGDYLSGPKVITETTKEAMREILDNIQSGNFANEFLDDCRQSNDGSGGPFMKSNRKSTKNHPIEEVGKELRSKMYDGKISEQEYFDNGVLMIAMVKAGVELAFDAMVASGIYEESAYYESLHELPLIANTIARKRLYEMNVVISDTAEYGNYLFSNVATPILAKEIIPNLQKGDLGEPTPAVEVDNITLRDVNDAIRNHPVELIGQELRGYMTDVLSLE",
    "response#2": "MKIYYDDDANLELIKKLNVTIIGYGSQGHAHANNLKDSGVDVTVGLREGSSSWSKASEAGLKVMPVDQSVLNADLVMILAPDEFQKGIFEKSIKPNLKSNAILAFAHGFNIHFKKIVPSETNTVIMIAPKGPGHTVRSTYLNGGGVPSLIAIFNDAMQEGEHSAKDIALSYAKANGGTRAGVLETYCETILEQTYFDHGVLMVAIIKASVELAFDIMVKSGIKPESAYYESLHELPLIANTIARKRLYEMNMVISDTAEYGNYLFSDFVLPLLKKEFVPYLKRGDLDLSHVNNIVHIDNILLRDINEMIRTHPIEKVGVQLRSYMKDVQAFK",
    "response#3": "MKIYYDDDANLELIKKLNVTIIGYGSQGHAHANNLKDSGVDVTVGLREGSSSWSKASEAGLKVMPVDQSVLNADLVMILAPDEFQKGIFEKSIKPNLKSNAILAFAHGFNIHFKKIVPSETNTVIMIAPKGPGHTVRSTYLNGGGVPSLIAIFNDAMQEGEHSAKDIALSYAKANGGTRAGVLETNFREETETDLFGEQAVLCGGAVELVKMGFETLTEAGYAPEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGTEVINEQSRVAMRNALKRIQTGEYAKMFIQEGKTNYPSMTARRRLNAVHPIETVGGQLRDMMPWANLENMVILPQIFVAEVKSDLMGEQTVLCGMLQVGSIFCFDKMVEDGLDSGYAGKLVQNGWEVITEALKQGGITLMMDRLSNVSKLRAFELSDQLKNILTPVFEKHMEDILDGSFSKNMMLDWINKDCKLLSYRQNNSQLLFESFKEECESDLFGEQAVLCGGLEAIVRMGFETLIKAGYPEELAYFECVHEVKLVADLLHYKGVEGLRKHISNTAEFGAIKAREPMGKLLKKRMQKILKKIQNGSFAKDFLLEKSLNYPRLNTERKALKETKIEQIGEILRAPFNHL"
  },
  {
    "reference": "MSAAPLPLPLVWTPPRGAKPPLHLADLTIEQVQKAVVTAGHPTYRAEQLSRHYFHAGVDQELTAVPRVAREALREQFLPPLLTAVHERVADRGTTRKTLWRAFDGTLIESVLMRYPDRATLCVSSQAGCGMACPFCATGQGGLTRNLSAGEIVDQVVRAAKTMRAGDLPGGSGRLSNVVFMGMGEPLANYNRVLTAIRRMLAPTPHGLGMSARGITVSTVGLVPAIHRLTDEGLALTLAVSLHAPDDELRNTLVPVNTRWPVREVLDAAWRYADVTGRRVSIEYALIRDVNDSVERADQLGALLAGRLTHVNVIPLNPTPGSKWDASSPQAQDRFVAAVGAHGVAITVRDTRGREIDGACGQLAASVTATPRSETMPALDLAAPGPTA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein with the reaction adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine, the Proton acceptor must be optimized to ensure efficient catalysis.2. The S-adenosyl-L-methionine, [4Fe-4S] cluster binding site should be stable and able to withstand changes in environmental conditions.3. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.4. [4Fe-4S] cluster is required for the proper folding and stability of the protein.5. The protein contains novel Radical SAM core domains that confer a unique function or activity.6. The designed protein should have rRNA binding, metal ion binding, 4 iron, 4 sulfur cluster binding, tRNA (adenine-C2-)-methyltransferase activity, tRNA binding within cytoplasm to regulate rRNA base methylation. The designed protein sequence is ",
    "response#1": "EEDRGTLCVSSQVGCTLTCTFCHTGTQRLVRNLTPGEIVGQILIARDRIGEWPGATRPTDGPLPNAERLITNIVLMGMGEPLFNFDNVRQAMEIAADGDGISVSKRRITLSTSGIAPMIPRCGVELGVMLAISLHAVRDDLRDVLVPINRKYPIAELLDACRAYPSLSNAKRITFEYVMLRGVNDSLADAKALVRLLAGIPAKINLIPFNPWPGAPYESSEWEQIEKFAEIINKAGYASPVKSPRGADIVEGYKHGNSVCISSQVGCRMGCRFCASTLDGLERNLSPAEMLDQIYRIQKITGERVSNIVVMGSGEPLDNYENLVQFIRLLTDENGLNISQRNVTVSTCGIVPGILKLAEEKFQITLALSLHAPNDEVRKSLMPVANRYPLADVLMACHTYFEKTGRRITFEYSLVSGVNDNLKEASALAALLKAQHGHINLIPVNPIKERDYVQSDRKAIEAFKNLLEKNGINVTIRREMGRDR",
    "response#2": "MDKRPLIHGLSKSEIGQIVASLGQPSYRATQIWEWLYKRMAFEWNAMSNLPLALREHLDRELAICPGTLTARSADASGSEKLLLTLRDGECVEQVLIPAPGRKTVCVSSQVGCRFKCAFCASGQAGFRRNLEAGEIVAQTLWAALIWERRPSNVVFMGIGEPLDNYDNVLKAIRIINDSEGLGIGARKITISTSGIIPGMERLAGEGLQIELSVSLHAPTQELRSRLMPVARLYPLDRLIRACRSYFEKTKRLITFEYVLIRGTNDQASHARKLAELLSDFPCRVNLLLLSPVPEFKGTSSSPDAANAFIKTLSRAGINATLRAPRGLSVNAACGQLRSSG",
    "response#3": "EKTDILSLTLPELELEMIKLGEKKFRAKQIFQWLHVKQIRDFSEMSNISLELRTKISGHFYLKSLNMVRRLESCIDNTVKYLYGLSDGTFVETVLMEYNHGLSICVSTQVGCRMGCKFCASTIAGFERNLEPSEILLQIYETQRDSGKRISGIVLMGIGEPLDNYKNVIKFLELVSAPEGFGLSLRHVTLSTCGIVPKIRELADLKLGLTLSVSLHCPDNDGRSEIMPVNKAYNLTELIEAAKEYVRKTGRRVTYEYAVIDNVNSFKKDAEKLAHLIKGINCHVNLIPVNSVKERDYRSSRESALKFQKYLTDLGINATVRRTLGSDIDAACGQLRREAE"
  },
  {
    "reference": "MVKSARQGAGGFWRDFKDFILRGNVVDLAVAVVIGGAFTSIVNAFVAWLMAVLLQPVLDQAGVSQLQDLPLGLGELVIAIINFLIIAFVIFLIIKAIEKMQRKKAVEEEIVAEAQPDPVLEAQTNLTDSINRLITTLENQQSSSQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. For general function, the protein need meet that Channel that opens in response to stretch forces in the membrane lipid bilayer; May participate in the regulation of osmotic pressure changes within the cell.3. The ideal protein would be one that plays a critical role in ion transport, cellular water homeostasis through mechanosensitive ion channel activity within membrane, plasma membrane. The designed protein sequence is ",
    "response#1": "HINPAVTFG",
    "response#2": "SGGGR",
    "response#3": "GKGNVVDLAVGVIIG"
  },
  {
    "reference": "MSTEGPHPAAGTGETLDPVEQAVADLAAGRPVVVVDDADRENEGDLVAAAETITTETVAFMMAQCRGLICVPMAGEDLDRLRLPQMVPDNTDSMGTAFTVSVDATAAHGVTTGISAADRAVTIRLLADPHAAPGDFTRPGHVFPLRARDGGVRERPGHTEAGVELARLAGLRPAAAIVEIAGEDGVMLRLPQLIPFARRHGLTVISIEELARTLAADGATPPDPRPAGSGTGTASPAPPLPGTPAHTAATAAVPLLREGETALPTSHGDLRAYGYRAADGVEHIAVVAGEIGDGEDVLVRVHSECLTGDVLGSLRCDCGPQLRDALDRIRAEGRGVLVYLRGHEGRGIGLTAKLRAYQLQERGRDTLDANLELGLPADARDYSAAAAILAELGVRSLRLLTNNPGKSAALGDHGLRVLERVRSTAGAGRHNLRYLRAKRDRMGHDLPWLDAPAGAAGS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.2. The GTP binding site should be stable and able to withstand changes in environmental conditions.3. The protein must have high specificity towards magnesium ion binding. The designed protein sequence is ",
    "response#1": "ESLLPIKNRGRFTIKTFRSRVDHLEHVALINQKIEEDKPCLVRVHSECLTGDVFGSARCDCGGQLEAALDEIATQGGVLLYLRQEGRGIGLANKIKAYALQEAGLDTVEANHHLGFGADLRDYGLAAQMLRVLGIREIRLLTNNPNKVESLTRYGISVAERAVETTLPTDFGTFNVYGYTNDLDDKEHIALVKGDIHENAPVLARVHSECLTGDVFSSHRCDCGPQLHEALNKINEAGAGVLVYMRQEGRGIGLINKLRAYQLQDAGMDTVEANEQLGFAPDMREYGISAQILKDVGAGKVNLLTNNPEKMRGLQSHGIQINARIS",
    "response#2": "RAAETIIPTAMAGEFKAIVYENDVDDLLHFVMIKGEIDPEKPILVRVHSECLTGDIFGSLRCDCGDQLHKTVKMMEEEGAGILLYIRQEGRGIGLINKIKAYALQDQGMDTVEANLELGFKADLRNYGIGAQILADLGVKKMRILTNNPKKIVGLEGYGLSIVEQVL",
    "response#3": "ASSTIPFKKIGELEMSVYKDNFSGDEVVVLSKPYSGNNPLVRMHSSCITGDIFGSLRCDCQDQLHKGIEMISEEGGFFIYLDQEGRGIGLTNKLKAYNLQMNENMDTIEANLALGLPADARKYDLAIQVLKYNNVNRCRLISNNPEKLAALRNVDILS"
  },
  {
    "reference": "MSIEVTQDGKAIKLDSVERAIADIRAGKAVVVVDDEDRENEGDLIFAAEKATPELVAFMVRYTSGYICAPMLPDDLNQLGLPPMAAVNEDVRGTAYTVTVDFNDGTTGISAASRAKTLRALASADAHREQFTRPGHVVPLSARPNGVLDRNGHTEASVDLARLAGLRPAGVICEIVSEKDPTDMARLPELREFADTHDLALISIEQMIQWRRRNEMQVVREVATSLPTDAGPMTALGYRHQVDGTEHVALIAGSVDELHNATDVPVRVHSECLTGDVFHSRRCDCGQQLDQAIDYIQRAGSGVVIYIRGHEGRGIGLLAKLKAYRLQESGADTVDANLEQGLPADAREFSVAGQILADLGIASANLLTNNPFKAEALEGFGVEVSKRTALLTEANEDNIAYLRTKRDRMGHDLPTVADWDVQRPQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a high affinity for Mg(2+).2. The designed protein must possess GTP binding to support riboflavin biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "EVAISLPTEYGDFKAVGYTNTIDGKESVALVKGDVGNGEPVLVRVHSECLTGDVFGSKRCDCGPQLHAALKQIEAEGRGVLLYMKQEGRGIGLINKFRAYKLQEEGYDTVEANERLGFPADLREYGIGAQILRDLGIRDMRLLTNNPRKIAGLEGYDLNIVERVK",
    "response#2": "AETVLPTAFGEFRAVCFVNDIDDYEHIALIKGEIDPEKDIMVRVHSGCLTGDVFGSLRCDCGEQLKKAMAKVQEEGRGVILYLQQEGRGIGLANKLRAYALQDKGLDTVEANEELGFDADLRDYGVGAQILVSLGVRKMRIMTNNPKKIIGLEGYGLEVTGRVS",
    "response#3": "VTSGMGEGRHYISLSGYMRQFRERLGYEPFPGTLNVDLDDGSVRTRARMDALDPVKIDGWEDDERTYGPAFCWPAVLETADGDRYGTAHVIAPERTHHGDDQLEIIAPEKLRETLSLDDGDTLSIHR"
  },
  {
    "reference": "MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPGEYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGNVSDGLKVWGVAGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAPVDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEAYGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRITDFTASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIRPASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRGKRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAVRYAVEQAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. We are looking for a protein with broad substrate specificity for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.2. The designed protein should be optimized for ATP binding activity in order to improve regulation of cell shape, cell cycle.3. Ideal protein should have a binding affinity for key enzymes in the Cell wall biogenesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MPITDVKNDSRQIKPGDLFIAYPGAAADGRLFIEKAVSLGAAAVAYEPDNFPPSCILPSSVPCVAIPHLAEQLADIAKRFYGQPSRKLEMVGVTGTNGKTTTTHLIEFLLGKAGVTGTNGKTTIAYQLAQAHHLLGQGAAYIGTIGQGDVNQLKPLDNTTPDALCLQKLLHQYKQQAIKQVCMEVSSHALSQHRVDAIEFKQAIFTNLTLDHLDYHHTMEAYGAAKALLFATPSLEWAIINQDDHYQQLMSEAVNPLAKKITYGMHQECDVKAINWRIDINGTDIEVRSPWGNHQVHIKALGEFNIYNSLAIYGTAVLLGLEKDEILQKLSGITTVPHRLEFFYEKGGLRFYNDSASTVPEATIAALEAFDESVVLIVGGTDKNADYQPLAQAAAKAHALYLLAGSATARLQPLLHAAGHETYQEIKGVKYPFDDREVAREILKELGYIG",
    "response#2": "MRLAELAGELGAPAPGSAVDVVSDSRRVQPGDVFLAFRGEYADGRDYIAAALERGAAAVLWDSAGAVPEGVPVVVVPDSKRALGPLAAAVHDRPTDALTLAGVGVTGTNGKTTTTHLIEFLLGKAGVTGTAGKTSIATFTRQIWERTGRAAASIGTTGVVSPKRVVEGALTTPDPVQLAALMAQLAGDGVTHAAIEASSHGLDQRRLDGIRLSAAAFTNLGHDHLDYHPDHESYFAAKMRLFTTLLPKGAPAVINADDAWSGRAIEVATGVSAKVMTVGRAGSFLTLKRLEHERSRQIAEIEAEGRIHRIVLPLAGSFQMANALVSAGLVIATGGDPRIVFDSLGRLQPVRGRFDYRVSASGVIGIVDYAHTPDAIQNVLNTIGDIRKGTEKVITVIGCGGDRDKSKRPVMASVACDWSDKVILTSDNPRTEDPAEIIKNMEAGVSPSNQKKCISIVDRKEAIKTACHLAQSGDILLIAGKGHEQGQDIAGVVRPFDDREVLRAAIEAASTAAAA",
    "response#3": "MKLVTGTNGKTTTSHLIEYFLNQQQRSSALLGTLYTRWPGYQKTATHTTPFATDLQKQLAEALQAGNQYAVMEVSSHALAQGRVLQCGFACAVFTNLTQDHLDFHGTMENYFAAKALLFKESYLQGRAVINQDDPYGQRLIDRLPLDQVYTYSVNDSTADFYTKDLDYQPTGVKGTFVTPQGEFPFLSPLVGQFNLANVLAAIACCVEAGFDLAALIEALPQFSGVPGRMEACKRPEAPLVLVDYAHTSDALEVALKAIHAHASGRVICVFGCGGDRDTGKRPMMLAVAQQHADYVWLTSDNPRTESPQSIIKDALAALDDTLAEVRVEVDRHQAIAQAVAMARPEDIVLIAGKGHETYQEINHVRHPFDDREWARLALKHRN"
  },
  {
    "reference": "MAAPPGEAPLTAATNIEPFYVLHKGGAAASSSSSSAPSLPSSGRARRRIDVSGLASPNPKPGKRSRDDDAAEDDDDDELYERLRLDAFHRVWSKIQSTINEVLRGISLKLFDQVLRWVQESFSAVRSIARPSAAEVRQPYPLLTDVICRKIPTAFVLTKNAEFVDDITTFRDLAEYLESNGCHLAKLSATELSEKNGVGCCFRSLLRQLLSDVPDVADIFALASWYSAAENYDQPIVVVIDDLEQCSGDVLGELVMMLSEWVIKIPIFFVMGIATTLDAPRKLLSSEDLQRLEPCKLTLGSPSDRMNALVEAILVKPCAGFCISHEVAVFLRNYFFKHDGTITSFISALKLACSKHFSVEPLSFLCMGMLEEDRENFWHDKFNALPQELRKYASGLPSCTREKDSTKSGDNMVDGLSELMNIQKDWSSVLLCLYEAGKHGKVQLLDIFCEAVNPDLHTQKAPNLPNEKSGTSRRFIDQVMDTIRYLPVETLFCLLEVWSIHLNGMDKITNKVKELQSTTISTDSVRITKDKWPRRSTNSTGNSTVALNDKVAMLLDDVTRKFLVSVECLPFHEIVCFKNVSILQSALIGNPRRMVQLDLVKSHKHLKCSCCRKNGIAVLASMHDTSIMCNLAQEYGDVINLHDWYISFDGIINSVHSKIKRKPHTSPSKKKSKPVAAESEAMIQARFCRAVTELQITGLLRMPSKRRPDLVQRIAFGL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein localizes to the nuclear pre-replicative complex.2. The protein must exhibit the following characteristics: DNA-binding is ATP-dependent. The designed protein sequence is ",
    "response#1": "MIFWGPPGTGKTTLAEIIAQRINAEVERISAVTSGIKEIREAIDRAKQNRLADRKTILFVDEVHRFNKSQQDAFLPHIEDGTVIFIGATTENPSFELNNALLSRARVL",
    "response#2": "PWNFQVIFYSSSKFFEKSFIFLECIQIILKKQSKRDNVSLWFVGPHPVFSLKDRKKCFYQLLIHSPSRTYLKKILKESINIVQCFSISQNVQWFLDII",
    "response#3": "PWNFQVIFYSSSKFFEKSFIFLECIQIILKKQSKRDNVSLWFVGPHPVFSLKDRKKCFYQLLIHSPSRTYLKKILKESINIVQCFSISQNVQWFLDII"
  },
  {
    "reference": "MTISRTPKFSAGELNVYSAPADVAAVTRALRTAGRRIVLVPTMGALHEGHLTLVRAAKRTPGAVVVVSIFVNPLQFGPNEDLNAYPRTLEDDLTALRAEGVEIVFTPTGSDMYPDGTRTSVHPGPLGDDLEGSSRPGHFAGVLTVVLKLFSIVRPDRAYFGEKDYQQLTLLRQMVADLNVDVQIVGVPTVRESDGLALSSRNRYLDKDQREQAGALSAALLAGKYAAAGGAEAALDAARAVLDEVPALEVDYLQVRDPMLGPAPAEGQARLLVAARLGRTRLIDNIAIDVGASAGIDGHPRVGNDQNHELPWRN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The beta-alanine, ATP, (R)-pantoate binding site should be stable and able to withstand changes in environmental conditions.2. A protein that localizes to cytoplasm and can perform ATP binding, pantoate-beta-alanine ligase activity with high specificity and efficiency.3. We require a protein that can specifically bind to the metabolites produced in the (R)-pantothenate biosynthesis, (R)- pantothenate from (R)-pantoate and beta-alanine: step 1/1, Cofactor biosynthesis pathway.4. For general function, the protein need meet that Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.5. The Proton donor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "MSFVPTMGALHRGHQSLIERSLKENSHTIVSVFVNPTQFGANEDFSAYPRPLEKDLALCEKLGDEASLVDVRPRGVDLVLRVGDDRHYEAAVFPLPDDGELQPGHLHNTGDWAALWLEPEAKAILVEEKAVLEARLAEAA",
    "response#2": "MNFVPTMGALHRGHQSLIERSLKENSHTIVSVFVNPTQFGANEDFSAYPRPLEKDLALCEKLGVPYVLLDKPSLLGKEVFSTVVMTMGALHEGHISLVKLAREQAGTVILTIFVNPTQFGPNEDENLLYFLKPTVEKVVLLTPGLSKKIGEAVAKVLKPGDYVVLSGDLGAGKTTLTRGVAQGLGVEEVVTSPTFVILNIYQGRLKVYHIDLYRVKDPEELEFIGFEEALEGKGVCLIEWPEKAAGLLPKAHLRVFLERLEDGTRRLKAE",
    "response#3": "MFVPTMGALHRGHQSLIERSLKENSHTIVSVFVNPTQFGANEDFSAYPRPLEKDLALCEKLGDFPIIPCNLCGSQPNLQRQKVADMLREWDREHPGRLETIFRALQNVQPSQLADRELFDFAGLTDGERPPYDGEDFAPFVPTMGYLHEGHIALLKKAREENDIVALSVFVNPLQFGPNEDFARYPRDIERAPFMRD"
  },
  {
    "reference": "MTARPHISILVVAAGRGSRAGGGLPKQYRPVAGRPLLAHSLEALCRAAPDASILPVIHSDDLGLYSQSVAALDRSYAARLREPVFGGATRQASVRAGLEALASGPSAAPEIVLIHDAARMFSGEALLSRALDAAIAHGAAIPGIAVTDTIKEIDSSAGVVATPPRSNLRAVQTPQAFRFDLILEAHRQAAAAGQDELTDDGAIAEWAGHRVHVFEGEPGNMKVTSAEDLVLAEARFIRELPDIRIGQGYDVHAFGTGDHVWLGGVKVPHDRGLVGHSDADVLSHAITDALLGALAEGDIGSHFPPSDPQWRGAASKIFLAHAAERVRARGGMIANIDATLICERPKVGPHRDAIRESLAAICGVPAERVAVKATTSEKLGFTGREEGMAALALATVRLPM",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design should be able to enhance the efficiency of terpenoid biosynthetic process.2. The protein design should incorporate a binding pocket that specifically recognizes a divalent metal cation to enhance enzymatic efficiency.3. The designed protein should interact with enzymes and substrates within the isopentenyl diphosphate biosynthesis via DXP pathway, isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6 pathway to enhance or regulate its activity.4. The designed protein should have a high specificity for 4-CDP-2-C-methyl-D-erythritol 2-phosphate binding, with minimal off-target effects.5. The presence of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MLPVVGWGYDVHRYGTGRPMKLGGVPIPGAPEVVAHSDGDVLLHALTDALLGCMGGGDIGEHFPDTAAEFDNIESGILLNEVYDQLLQQGIVVTHVDVTVIAQVPKVSPWKPHIRKNLCRLLKLAPEQVNVKATTEEKLGFTGEKKGIKAVATVTAML",
    "response#2": "TITGTGYDVHKLIPGKAMMLCGVSIPGDLALLGHSDADAALHALTDAILGTIGAGDIGDHFPPSDPQWRGTDSKVFLAKAMVLLARQSARLLHVDLTIICERPKIKPHRQAMRARLSELLDLPLARISTKATTTEGLGFTGRGEGLAAMATVSVQRVGIGTDVHAFGGEGDLWLAGLPWPGEQALSGHSDGDAVAHAIVDALLGAAGLGDIGEHFGTAHPEYAGAHAAVFLSRTRELLTDAGFVIGNVSAQFQGNRPRFSARRAEAEAVLSEALGGAPVSVTATTTDGLGFPGRGEGISVTAIALVL",
    "response#3": "REDLDRAERIMKMSKPDHEPAPLSRLDDVRMGTGYDVHALEPGDGIMLCGVKIPFDKRLKGHSDADVAQHAVTDAILGAIADGDIGMHFPPSDPQWKGASSDQFLKDAIRRVRQLGGQIAHIDVTIVCEAPKIGPHRDALRSSMAKITGLDRSRISVKATTSERLGFTGRSEGIAAMASATG"
  },
  {
    "reference": "MDGGGVDTATTAAWMEKHRHMYERATRHPFTVSIRDGTVDMSAFKRWLSQDYLFVREFVAFIASVLLKCCKQEDSSDMEIILGGVASISDEISWFKNEATVWGVDLASVSPLKANLEYHRFLRSFTEPEISYAVAVTTFWTIETVYQDSFGFCIQDGNKTPPELLGTCQRWGSAGFRQYCQSLQSIVDRCLANAPADAVQSAEEAFVRVLELEIGFWDMSSSRS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be optimized for thiaminase activity.2. The designed protein must have a Proton donor active site that is highly conserved among related enzymes.3. The protein must exhibit the following characteristics: Does not display thiaminase II activity, as it is unable to hydrolyze thiamine; May also serve a damage pre- emption function by hydrolyzing products that would otherwise do harm (Probable).; Has a high formylamino-HMP amidohydrolase activity.4. The protein should be optimized for activity in thiamine diphosphate biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "DPSGGAGIQADLKSIAANGGYGMAVLTALTAQNTRGVRGVHVPPAEFLRAQLDAVSDDIVIDAVKIGMLADASVIRTVSAWLDDVRPPIVVLDPVMVATSGDRLLDPAAERELRDLLGRATLITPNLAELAVLSGRALADWEDAQSAAEELSRDVGAAVLVKGGHLDGDDAPDALRDARHGLRQEFPGERIPSRNTHGTGCSLSSALATRLARGDDPVAAVRAARAWLRESLRAGESLRVGGGTGPII",
    "response#2": "DPSGGAGIQADLKSIAANGGYGMAVLTALTAQNTRGVRGVHVPPAEFLRAQLDAVSDDIVIDAVKIGMLADASVIRTVSAWLDDVRPPIVVLDPVMVATSGDRLLDPAAERELRDLLGRATLITPNLAELAVLSGRALADWEDAQSAAEELSRDVGAAVLVKGGHLDGDDAPDALRDARHGLRQEFPGERIPSRNTHGTGCSLSSALATRLARGDDPVAAVRAARAWLRESLRAGESLRVGGGTGPIV",
    "response#3": "DPSGGAGIQADLKSISAVGGYGMAAITALTAQNTRGVRDVHVPPAAFLTAQLDALADDLAIDAVKLGMLANADVIRAVGDWLRRTRPPVVVLDPVMVSTSGHRLLDADAEQALRELLPLADLVTPNLAELAVLAGESPATDWAGVVAQAETVSAGFGVRVLAKGGHLGGDTAPDALVDAASATIVEFGGDRIATTSTHGTGCSLSSAIATLRVSRGDWEPAVAQARRWLRESIRHGGDLAVGGGHGPVV"
  },
  {
    "reference": "MAWRRHALLCPLGLCLVIWPLLLPAGGCGPGRGPVGRKPYARKNLTPLQYKQFVPNVAEQTLGASGKAEGKITRRSDRFKDLVPNYNPDIIFKDEENTGADRLMTERCKDRVNALAIAVMNMWPGIRLRVTEGWDEDGHHLPNSLHYEGRALDITTSDRDREKYGMLARLAVEAGFDWVYYESKAHIHVSVKADNSMAIRAGGCFPGDAKVTLQSGETKDLSELRRGDYVLSVDHQSGQVMPSEVLLFLDRDQDRQATFVAIETDTPWSQLLLTPSHLIFAAQDPANSTADFSPVFASRVHVGDSVLAYQAGSPRLHPARVVRVWWQQGIGVYAPLTSHGTILVNGVLASCYAVLESHLWAHRAFSPLRLAHGLFALKDEARNCSIQETGVHWYSWLLYQLAWGVLGQETLHP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be involved in protein autoprocessing, system development, cell-cell signaling by localizing to Golgi membrane, endoplasmic reticulum membrane and exhibiting metal ion binding, peptidase activity.2. Include a signal peptide as an essential component of the protein.3. The protein should be designed to have high stability and activity towards cholesterol + glycyl-L-cysteinyl-[protein] + H(+) = [protein]-   C-terminal glycyl cholesterol ester + N-terminal L-cysteinyl-   [protein].4. The designed protein must have at least one Hint domain.5. The protein should be able to bind Zn(2+), Ca(2+) ligand in a variety of conditions6. For general function, the protein need meet that [Protein hedgehog]: The C-terminal part of the hedgehog protein precursor displays an autoproteolysis activity that results in the cleavage of the full-length protein into two parts (N-product and C-product); In addition, the C-terminal part displays a cholesterol transferase activity that results by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product. The designed protein sequence is ",
    "response#1": "CGPGRAPPGRRRPARRPLVPLRYKQSVPGVPEQTLAASGRAEGRLGRSAERFRDLVPNYNPDILFKDEEHTGADRLMSQRCKDRVNALAIAVINMWPGVKLRVTEGWDEDGHHLPDSLHYEGRALDITTSDRDREKYGLLARLAVEAGFDWVYYESKAHIHVCFPGDARVIVEGGRTKQMRDLRPGDRVLASSSTDINAPLLYSPIVSFLDRQPNATRTFYVIGTNGGHNITLTAAHLIFSSDSSQVGKTGGCFTGDSLVSTSDGRQLKLEDVRLGDELMSIDPHSGRVVYSEVILFLDRDPQETRPFLRITTSTGAVIKMTPSHLLLRVDSEKPLNLAAVFADSVKPGDSVLLANISGGEPIIDKVTHIDTFTTSGVYAPLTRTGTIVVNGVSASCYALINSQFVAHLSFAPLRIYLNLKESLMRLWTFASHPLTTNSWNHHNYLRKRRSFSIQSGIHWYPEFLYKISNILCFPGDARVIVEGGRTKQMRDLRPGDRVLASSSTDINAPLLYSPIVSFLDRQPNATRTFYVIGTNGGHNITLTAAHLIFSKSEHSVAAKTGGCFPGDARVIVEGGRTKQMRDLRPGDRVLASSSTDINAPLLYSPIVSFLDRQPNATRTFYVIGTNGGHNITLTAAHLIFVTDCATRPSSSGREEIFQETKPFGSMLQRRAHGAGMKTVYASEVQPGQCVFTSSGRSNSRPRLSVVTFVEEQTSTGLYAPLSQHGSIVVNGVLASCYATVDSHYLSHWVLAPLRLVYRIIGPSQVQNDGLHWYPWILQKLGKVL",
    "response#2": "CGPGRGGAGRRSRLRKLTPLVFKQHIPNVSENTIAASGLAEGRVERHSSRFHNLVPNYNPDIVFKDEEGTGADRFMTQKCLERLNTLAISVMNQWPGVRLRVTEGWDEEGQHAEDSLHYEGRAVDVTTSDRDRSKYGMLARMAVEAGFDWVYYESRSHIHCNKSDSSHTPHDSGCFTKDSTVLLQSGERKAMSDLVIGDQVLSMNAKGEAIYSEIILFMDRNLELAQEFVQLTTDTGVQLTVTPAHLVMVWNSAKQLADYVFADRVEEGDQVFVYDHTGLLRPQKVVDLKAVLRKGVVAPLTREGTIVVNSVAASCYAIVNSQSLAHWSLAPMRVWSALKERLFSTSSSAAKSQHTSTGQLQNGVHWYAKTLYSIKNFVKAENSVAAKSGGCFPGSGAVTLEDGRTKNVKDLTVGDKVLAADEEGNIVYSDFIMFLDHDPTTQREFLVIETSEPAKKLRLTAAHLVFVRNLSVDSGMTAKFASSVQPGHEVFVLDGARGHPKSVRVERIYTVEYTGSYAPLTAHGTIVVDQVLASCYAVIEDHRWAHWAFAPVRMNRWLTSWSLPTHDANVTLREDGIHWYSNLLFHIGTWLLSDSSQVGKTGGCFTGDSLVSTSDGRQLKLEDVRLGDELMSIDPHSGRVVYSEVILFLDRDPQETRPFLRITTSTGAVIKMTPSHLLLRVDSEKPLNLAAVFADSVKPGDSVLLANISGGEPIIDKVTHIDTFTTSGVYAPLTRTGTIVVNGVSASCYALINSQFVAHLSFAPLRIYLNLKESLMRLWTFASHPLTTNSWNHHNYLRKRRSFSIQSGIHWYPEFLYKISNIL",
    "response#3": "KSEHSVAAKTGGCFPGGARIITEGGVTKTMRDLRPGERVLASSGVDGRGELVYSEVLAFLDSDPEARKHFYVIGTEAGERLPLTAAHLLFVSEGNCSGWVAGSGLRAVFASDVQPGQCVVSAADGRGRLSRVAWVHIREERGVFAPLTRHGTVVVNGVLTSCYAVMDQHHLAHWAFAPLRALYSWVGPLGRRADGIHWYSSALYWIGTLLKSEHSVAAKTGGCFPGDAQVTVEGGGTKLMRELLPGDRVLASSSAEGHAQLLYSPVLSFLDHQPNITKTFYIIGTNAGLSISLTAAHLIFISDCAGGPNKWNESAGFGSTSQGHGGGPRTVFASDVRPGQCVFTSTGKANWHTRLSVVTFVKEKRSTGLYAPLTQHGSILVNGVLVSCYAAVDSHQLSHWALAPLRLFYRLFGPPRAQNPGLHWYPWILQSLGQVLKSEHSVAAKTGGCFPGGARIITEGGVTKTMRDLRPGERVLASSGVDGRGELVYSEVLAFLDSDPEARKHFYVIGTEAGERLPLTAAHLLFVSEGNCSGWVAGSGLRAVFASDVQPGQCVVSAADGRGRLSRVAWVHIREERGVFAPLTRHGTVVVNGVLTSCYAVMDQHHLAHWAFAPLRALYSWVGPLGRRADGIHWYSSALYWIGTLLGGCFPGHATVTLRSGERKGLSELRRGDWVLAVDRSGQVVPTEVLLFLDRDPHRRVPFVAIETESPAHRLLLTPSHLVFAAQAPANGTAGFLPVFARRVRAGDSVLAHLCFPGDALVTTETGGRTALRDLQPGDRVLTATFDPAHLLYSEVLTFLDRDPVSRKLFYTVHTHSGAALSLTAAHLLF"
  },
  {
    "reference": "MSLMDPLADALTNIRNNELQVKDSCVISPASKLIGEVLSTMQKENYIGNFEYIDDNRAGQFKVELEGNINKCGVIKPRHAVKKDEFEKFEKRYLPAKNFGILIVTTPEGIMTHYEAKERGIGGRLLAYMY",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that contributes to translation by functioning in ribosome, ribonucleoprotein complex with structural constituent of ribosome, rRNA binding. The designed protein sequence is ",
    "response#1": "LESRLDNLVYRLGLARTRRQARQLGV",
    "response#2": "MRRLDAVVYRAKFVSTMFAARQFINHGHIKVNGKRVNIPSYKVRVGDVIEVKEASKQLAFVLEASS",
    "response#3": "LESRLDNLVYRLGLARTRRQARQLG"
  },
  {
    "reference": "MPKKTKFIFITGGVVSGLGKGIASASIGALLESRGLTISLQKFDPYINVDPGTMNPFQHGEVFVTDDGAETDLDLGHYERFTTVRTTHLSNFTTGKIYDSVIRKERRGDYLGRTVQVIPHITDEIKASIMKAASDVDLVLVEIGGTVGDIESLPFLEAIRQFRADVGRENALYVHLTLVPFIEASGELKTKPTQHSVQKLREIGIQPDILLCRSHEPLPKEVKQKIALFCNLPADCVKTALDVENIYDVPLVYHREGLDEKIVELLGIWTKAPNLRSWEDLGRKIRNPTSEVTIGIVGKYIDMTDSYKSLNEALKHGGIANAATVKLHFIDAGTLENTASSLETLLQDVDAILVPGGFGERGILGKIRAIQYAREKKIPFYGICLGMQLAVIEFARHVCGLSHANSLEFDANTPDPVISLMEEQKAVHNKGATMRLGSYPCTLEKGTTSIAAYGEKHIEERHRHRYEFNNAYKSALKEQGMIIAGVSEPAGLVEIIEIEDHPWFVGCQFHPEFKSRPQTPHPLFSHFIQAAIGYSRRREWSQQKEEKNLELKSPPTPVKAQKSNS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be optimized for metal ion binding, ATP binding, CTP synthase activity, glutaminase activity activity in order to improve glutamine metabolic process, 'de novo' CTP biosynthetic process.2. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen3. The designed protein should have a high specificity for CTP, L-glutamine, ATP, UTP binding, with minimal off-target effects.4. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile, active site, Nucleophile; for glutamine hydrolysis to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).5. The protein contains novel Glutamine amidotransferase, CTP synthase N-terminal domains that confer a unique function or activity.6. A protein should be able to manipulate the activity of enzymes in the CTP from UDP: step 2/2, CTP biosynthesis via de novo pathway, Pyrimidine metabolism pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "MAKYIFVTGGVVSSLGKGIAAASIGCLLESRGLRVTLQKFDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTYAPLTQSNNVTSGRIYETIISRERRGDYLGKTVQVIPHVTDEIKANIEKVSGGVDVVLVEIGGTVGDIESLPFLEAIRQLRHKKGRENCFFVHVTLVPWIAAAGELKTKPTQHSVKELRTIGIQPDMLICRCEKPLAGDIKDKIALFCDVSADAVIEACDAKSVYEVPLGFASQNVDDIILRRLNLPFRDVDWESWQELLDRVHHPQHELPDSYKSINEAFVHAGAQNDCKVNVKWISSEEINPETCEDILAGLDGILVAPGFGERGMEGKIEAIRYVREQKIPFFGVCLGMQCAVIEFARNVLKLHGASSTELDPNTRHPVIDLMEEQKKVTEKGGTMRLGAYACRLKKGSKAYNIYGESIIHERHRHRYEFNNKYLNDIEEAGLKAVGVNPESGLVEVVELKDHPWFIGVQFHPELKSTVLNPHPLFVKFVFVAAAGQLSEQGVQFPVPELE",
    "response#2": "MTKYIFVTGGVVSSLGKGIAAASIGCLLESRGLRVTLQKFDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTYAPLTQSNNVTSGRIYETIISRERRGDYLGKTVQVIPHVTDEIKANIEKVSGGVDVVLVEIGGTVGDIESLPFLEAIRQLRHKKGRENCFFVHVTLVPWIAAAGELKTKPTQHSVKELRTIGIQPDMLICRCEKPLAGDIKDKIALFCDVSADAVIEACDAKSVYEVPLGFASQNVDDIILKHFGIEAPEVDMSEWCEMIDRIKNLDGEVKIGLVGKYVELKDAYKSIVESFVHAGAMNECKVKISWTKAEDLADGDLDLLKGLDGILVAPGFGERGFEGKINAIRYARENKIPFFGICLGMQCAVVEFARNVLQLKEATSIEMNPNTSEPVINMMEDQKRITQMGGTMRLGAYPCHIKKGTKAFSVYGKTEISERHRHRFEFNNDYLKRYESAGMIASGINPASGLVEIMELKDHPFFIGTQYHPELKSTVLNPHPLFVKFVAASIENSKLV",
    "response#3": "IFVTGGVASSLGKGLTASSLGMLLRARGLSVTMQKLDPYLNVDPGTMNPFQHGEVFVTDDGAETDLDIGHYERFLDENLDASANVTTGQVYSTVIAKERRGEYLGDTVQVIPHITDELKNRMRRRAQGDDQPDIIITEIGGTVGDIESQPFLEAARQVRQDVGRRNVFYLHVSLIPYIGPSGELKTKPTQHSVAALRGLGIQPDALVLRSDRQVPDTMLRKIGNACDVDHDAVISCPDAPSIYDIPRTLHSQGLDTYILRHFGLPCGTANMSEWDAMLERVHHPAHELPDSYKSINEAFVHAGAQNDCKVNVKWISSEEINPETCEDILAGLDGILVAPGFGERGMEGKIEAIRYVREQKIPFFGVCLGMQCAVIEFARNVLKLHGASSTELDPNTRHPVIDLMEEQKKVTEKGGTMRLGAYACRLKKGSKAYNIYGESIIHERHRHRYEFNNKYLNDIEEAGLKAVGVNPESGLVEVVELKDHPWFIGVQFHPELKSTVLNPHPLFVKFVFLKAAAAYDRERES"
  },
  {
    "reference": "MATRPKVRRPDRALYVPPKVDRIKRVIFACGHRKRCEIGYRPLGDSGNCGPCGARRDVFAPDLYGSGPLPPCPVCGRAVVGPTVREACGHVTCNACETEACAVDRLCIGGGRRLVAICDPYPPYPGPRWRGPRPTRPEAHEAVQRSRGSSEDACTCAP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must have high specificity towards metal ion binding.2. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "AGTQEELLRWCQEQTAGYPGVHVTDLSSSWADGLALCALVHRLQPSLLEPSDLQGMRALEATAWALKVAEHELGITPVVSAQAVVSGSDPLGLIAYLSHFHSAFV",
    "response#2": "VAAPVLSTLFPMAAPLIGAADQFIGGLTKTNAAGGRYLTHAAGGRYTDVMDSWASGTDTGRFSRNLKDRLESNNYEEMELPPPTKGVIIPVVHTVESAPGEAFGSLLVIIPGAYPELLDPNQQVLSHFKNDTGCVWGIGEDIPFEGDDIWYTALPLKEIKKNGNIVVEKVFAGPAMGPSCQLGLSLLVNDIDKGVPRMVFTGEIANDEETIVPICGVDIKAIAAHEHGLPLVGCQPGVDEVVANTSLASHLL",
    "response#3": "AGTQEELLRWCQEQTAGYPGVHVTDLSSSWADGLALCALVHRLQPSLLEPSDLQGMRALEATAWALKVAEHELGITPVVSAQAVVSGSDPLGLIAYLSHFHSAFY"
  },
  {
    "reference": "MEPLLLGRGLIVYLMFLLLKFSKAIEIPSSVQQVPTIIKQSKVQVAFPFDEYFQIECEAKGNPEPTFSWTKDGNPFYFTDHRIIPSNNSGTFRIPNEGHISHFQGKYRCFASNKLGIAMSEEIEFIVPSVPKFPKEKIDPLEVEEGDPIVLPCNPPKGLPPLHIYWMNIELEHIEQDERVYMSQKGDLYFANVEEKDSRNDYCCFAAFPRLRTIVQKMPMKLTVNSSNSIKQRKPKLLLPPTESGSESSITILKGEILLLECFAEGLPTPQVDWNKIGGDLPKGRETKENYGKTLKIENVSYQDKGNYRCTASNFLGTATHDFHVIVEEPPRWTKKPQSAVYSTGSNGILLCEAEGEPQPTIKWRVNGSPVDNHPFAGDVVFPREISFTNLQPNHTAVYQCEASNVHGTILANANIDVVDVRPLIQTKDGENYATVVGYSAFLHCEFFASPEAVVSWQKVEEVKPLEGRRYHIYENGTLQINRTTEEDAGSYSCWVENAIGKTAVTANLDIRNATKLRVSPKNPRIPKLHMLELHCESKCDSHLKHSLKLSWSKDGEAFEINGTEDGRIIIDGANLTISNVTLEDQGIYCCSAHTALDSAADITQVTVLDVPDPPENLHLSERQNRSVRLTWEAGADHNSNISEYIVEFEGNKEEPGRWEELTRVQGKKTTVILPLAPFVRYQFRVIAVNEVGRSQPSQPSDHHETPPAAPDRNPQNIRVQASQPKEMIIKWEPLKSMEQNGPGLEYRVTWKPQGAPVEWEEETVTNH",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein's functionality will benefit from Polar residues, Basic and acidic residues compositional bias in its structure.2. The designed protein must have at least one Ig-like C2-type domain.3. The protein design should include a FIG[AQ]Y, DGEA motif to ensure proper function.4. The protein must exhibit the following characteristics: Plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of interneurons and the efficacy of GABAergic synapses; Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death; Recruits ANK3 to the plasma membrane .; Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity; May play a role in regulating cell migration in nerve regeneration and cortical development; Potentiates integrin-dependent cell migration towards extracellular matrix proteins.5. A protein that contributes to cognition, neuron migration, exploration behavior, cell adhesion, axon guidance by functioning in membrane, apical part of cell, dendrite, extracellular exosome with protease binding.6. Incorporate a signal peptide in the protein design.7. The protein should have a Helical domain that is long enough to span the entire membrane.8. The Extracellular topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "GEQGYEAEIDDDLEPFSTVLRVEASDADIGINSAIYFSLVNRSHDFIVEPVTGWVRSLRHVKPGKYSLKVKSEDRASRLYYFDENEVQPSWTADVLITVRETKPKPRRILVDQRKINPNHFECKVKCINYKGDYKCECTDLKIGFECTNIVKQPEYAVVQRGSMVSFECKVKHDHTLSLTVLWLKDNRELPSDERFTVDKDHLVVADVSDDDSGTYTCVANTTLDSVSASAVLSPTAKLSVIFLDSASVQLLGSNRNELLLKCHVEGASGNEALQIEWYRNSAKLSSWQNIELDEHRLLVRQPTGSDDGLYRCIASNAAGRVMPTRISAPPRSATAKKGETVTFHCGATFDPAVTPGELRWLRGGQPLPDDPRYSVAAEMTVSNVDYGDEGTIQCRASTPLDSAEAEAQLSVIDPEPTAKLSVIFLDSASVQLLGSNRNELLLKCHVEGASGNEALQIEWYRNSAKLSSWQNIELDEHRLLVRQPTGSDDGLYRCIASNAAGRVMSRPAFLRIIAGPGILEVIEQLKFMPQPTSKNLELDAAVAKVHCKAQGTPTPQVQWFRDGVNTTLPDQVEVDLNGTLIFRNVNADHRGNYTCLATNLQGQINATVSISATAPLR",
    "response#2": "KRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELNVTDIDECKDPDACSQICVNYKGYFKCECHPGYEMDTLTKNCPRLLLPRDPQTTTIALRGGSVVLECIAEGLPTPWVRWRRLNGPLLPGGVGNFNKTLRLWGVTESDDGEYECVAENGRGTARGTHSVKKSLIAYVGDSTVLKCVCQDCLPLNWTWYMGNETAQVPIDAHSNEKYIINGSHANETRLKIKHLLEEDGGSYWCRATFQLGESEEQNELVVEQRYEVVENQTVFLHCRTFGAPAPNVEWLTPTLEPALQDDRSFVFTNGSLRVSAVRGGDGGVYTCMAQNAHSNGSLTALLEVIVKQPEYAVVQRGSMVSFECKVKHDHTLSLTVLWLKDNRELPSDERFTVDKDHLVVADVSDDDSGTYTCVANTTLDSVSASAVLSVTEFMENGALDSFLPKDYIIDPRENIVIQCEAKGKPPPSFSWTRNGTHFDIDKDPLVTMKPGTGTLIINIMSEGKAETYEGVYQCTARNERGAAVSNNIVVRPPTRISAPPRSATAKKGETVTFHCGATFDPAVTPGELRWLRGGQPLPDDPRYSVAAEMTVSNVDYGDEGTIQCRASTPLDSAEAEAQLTVIVDPVAPPSRDLQVMEVDEHRVRLSWTPGDDHNSPIEKFVVEEEEEREDLQRGFGAADVPGQPWTPPLPLSPYGRFPFRVVAVNAYGRGEHHAPSAPI",
    "response#3": "LQANDEDTGYNGFIRYVIHDIAGSSHEVLTVDEQSGEITVASDLSKLMKEKLEVLDVQLKVSAIDAGTPVKSSVKTMKLRIKDVNNHSPTRIVRGPEDQVVKRGSMPRLHCRVKHDPTLKLTVTWLKDDAPLYIGNRMKKEDDGLTIYGVAEKDQGDYTCVASTELDKDSAKAYLTVLEPASSSQMVLRGVDLLLECIASGVPAPDIMWYKKGGELPAGKTKLENFNKALRISNVSEEDSGEYFCLASNKMGSIRHTISVRVPRSATAKKGETVTFHCGATFDPAVTPGELRWLRGGQPLPDDPRYSVAAEMTVSNVDYGDEGTIQCRASTPLDSAEAEAQLRVSVRDVPPDRPRDLELSDLAERSVKLTWIPGDDNNSPITDYIVQFEEDRFQPGTWHNHSRYPGNVNSALLSLSPYVNYQFRVIAVNDVGSSLPSMPSSEPGTAVTVQT"
  },
  {
    "reference": "MRWLCHRLQVSVSGFYDYIHRKPTENQKKRKKTAAYIVMQFKCLKGRMGYRKLYRYLVNKGVKVTLSQVRQALCKSGLQSKVVTYYKKRKQEHRTFPNVLKRSFKPGKKDIPTVVCDITEFRLMNGMKVYFCAALDISTRRVLGYSLDTCQDAQLVKDTIQQVKNTYGKRENILFHSDQGSQFSSYQVTDFCKQQKIQQSMSRKGNCWDNAVIESFFSIFKRECLHGEKLISLSQVNQLVAEFIYMYYHSVRPHSTLNGMTPYQYSKSIA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Involved in the transposition of the insertion sequence..2. The designed protein must have at least one Integrase catalytic domain.3. The protein design should be able to enhance the efficiency of DNA integration. The designed protein sequence is ",
    "response#1": "KPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRHGL",
    "response#2": "KPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRR",
    "response#3": "HGRLPLVMRQSHAGGEKLFVDYAGDKVAVVDRKTGVVRDAHIFVAVMGASSLSFALATWSEQLPDWIEAHNAAYRFFGGVTQLLVSDNTKCAVIKACHFDPMVNRSYTDMARHYSTAVFPARPRKPRDKAKVENCVGIVERWLLGRLRNRTFYSLADLNKAIADLITRLNDERVIRQYGKTRRALFDELY"
  },
  {
    "reference": "MRAKGIKRNYQYLWGIMLLGMLMICSTVAQWWVTVYYGVPVWKDATTTLFCASDAKAYETEVHNVWATHACVPTDPNPQEIRLENVTENFNMWKNNMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCSNLRNNTNTNSTSTNHTIGGEGDMKNCSFNTTTGIDNKIQKQYALFYTLDVVQIKNDTKSYTLVNCNTTVISQACPKVTFEPIPIHYCAPAGFAILKCKDEKFNGSGPCNNVSTVQCTHGIRPVVSTQLLLNGSLAEGGEVVIRSENFTNNAKTIIVQLDTRVEIDCIRPNNNTRRSIHIAPGSAFYATGDIIGDIRQAHCNISRAEWNSTLVKIAKKLREQFNTTIAFNKSSGGDLEIVMHSFNCGGEFFYCNTTQLFNSTWEAHQLNQTWNEPLGPNVTNITLPCRIKQIINRWQEVGKAMFAAPISGRIQCKSNITGLLLTRDGGTNTSDPNRTETFRPGGGDMRDNWRSELYKYKVVQIEPLGIAPTKAKRVVVKREKRAVGVLGAMFLGFLGTAGSVMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICHTAVPWNKSWSENISSKNISIDQIWQNMTWMQWEREIDNHTELIYNLLEQSQIQQEKNKQELLELDKWTNLLDWFDITKWLWYIKIFIMIVGGLVGLRIVFTVLSIVNRVRQGYSPLSFQTRLPVPRGPDRPEGIDEEGGERDRDRFGQLAPGCLALIWSDLW",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Design the protein with a signal peptide incorporated.2. The protein should have a specific function in fusion of virus membrane with host endosome membrane, clathrin-dependent endocytosis of virus by host cell, positive regulation of receptor clustering, actin filament reorganization and should be localized to host cell plasma membrane, virion membrane in order to carry out this function efficiently.3. The protein contains novel Retroviral envelope protein GP41-like domains that confer a unique function or activity.4. For general function, the protein need meet that Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; Transmembrane protein gp41: Acts as a class I viral fusion protein5. The protein design should include a YXXL motif; contains endocytosis signal motif to ensure proper function. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWKDAETTLFCASDAKAYKTEKHNVWATHACVPTDPNPQEIPLENVTEEFNMWKNKMVEQMHTDIISLWDQSLQPCVKLTPLCVTLNCTDVTNVTDVSGTRGNITIMKEMEGEIKNCSFNMTTEIRDKKQKVYSLFYRLDVVPINQGNSSSKNSSEYRLISCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCRDKEFNGTGECKNVSTVQCTHGIKPVVSTQLLLNGSLAEEKVKIRTENITNNAKTIIVQLVEPVRINCTRPNNNTRESVRIGPGQAFFATGDIIGDIRQAHCNVSRSQWNKTLQQVAEQLREHFKNKTIIFNSSSGGDLEITTHSFNCGGEFFYCNTSGLFNSTWNTSMSGSSNTETNDTITLQCRIKQIINMWQRTGQAIYAPPIQGVIRCESNITGLLLTRDGGEEKNSTNEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVGREKRAALGMLFLGFLGSAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLKLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTNVPWNSSWSNKSQDEIWNNMTWLQWDKEISNYTNLIYSLIEESQNQQEKNEQDLLALGKWANLWTWFDISNWLWYIRIFIMIVGGLIGLRIVFAVLAVIKRVRQGYSPVSFQTRLPAQRGPDRPEGIEEEGGERDRDRSGPLVDGFLAIIWVDLRSLCLFSYHRLRDLLLIVT",
    "response#2": "LWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPSPQEVVLKNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCINATASML",
    "response#3": "LWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPSPQEVVLKNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCINATASMD"
  },
  {
    "reference": "MSKIHVLNDKFENSGELELPASYAEVNPHNLYLYVKSYLAGIRANSAHTKSRAFVSGGGKKPWRQKGRGGARAGSTRTNVWVGGAVAFGPTNEKNYFQKVNKKQKRLALEYALAVKAQDGKIFAVDSISIESGKTKDAANIIKNLKVKDALIVKDLLDDKTLLAFRNLANCYVVDANEVNAYLVSTFSSVIIEKAALKTITKEG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have rRNA binding, structural constituent of ribosome and be active in ribosome.2. For general function, the protein need meet that Forms part of the polypeptide exit tunnel. The designed protein sequence is ",
    "response#1": "MF",
    "response#2": "MW",
    "response#3": "MEPIGAKVTLRGERMYDFLDKLVNVSLPRVRDFHGVSAKSFDGRGNYTLGVREQLIFPEIDYDKVDRVRGLDVVIVTTSNTDEEARELLTQFGMPD"
  },
  {
    "reference": "MKKTVADQFVEVLALAGVKRIYGVVGDSLNALTESLRRRGDIEWVPMRNEEAAAFAAGAEAHLTGQLAVCAGSCGPGNLHLINGLYDCHRSRAPVLAIAAQIPSVELGTGYFQETHPESLFKECSHYCELVSGPNQMQRTAEIAVRSAVGKGGVAVVVLPGDIAMLKAEDARAPTPESLRASPSQLMPSSAQVEQMAALLNRGGRVTLLCGAGCEGAGAQVVELAKRLQAPVVSALRGKEFVEKDNPHFVGLTGLIGYASGYWAMIDCDVLLMLGTDFPYRQFYPDSADTRIIQVDVRAENIGKRTRVDLGVVGSVSATVQALLPRIEAKRDTKHLERALAHYRKTREELDALGQGTAGKKPIYPPQVARAFDLQAADDAIFTCDVGLPTVWAARYATMKGGRRLVGSFNHGSMASALAQAIGAQKAFPDRQVIAFSGDGGFTMLMGDFIGLVQLGLPIKVVVFNNSSLGFVAMEQQVGGMLDVGTALQNPNFAALAEAVGIKGLRVEDPAQVDEAVQLALATPGPVLVDAVVSKAELVMPPRITASMAAGFTLFGIKALLNGRTNEVLELARTNLWR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have flavin adenine dinucleotide binding, pyruvate dehydrogenase (quinone) activity within plasma membrane to regulate pyruvate catabolic process.2. The presence of Thiamine pyrophosphate enzyme central domains is critical for the interaction of the designed protein with its intended targets.3. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "LEEAVSVLAGSRRVTILAGSGSADAHDEVVALADRLAAPVVHTLRSKEHLEHDNPFDVGLTGLLGFASGYRAMMEADVLLMLGTDFPYRQFYPQDATVIQVDLRGEHIGRRTRVDLPIVGTVRDTAAAIRAAD",
    "response#2": "MHPQYLTKVVSDAAAADAIFTCDVGTPTVWAARYLEMSEQRRLIGSFNHGTMANALPQAIGLQAAERERQVISFSGDGGLSMLMGDLITLKQHNLPVNVVVFNNSALSFVELEMKAGGYLESGTDLHQTDFAAVANALGIQGIRVEDPEELEDAVQRALAHDGPALIDVVVNRQELPLTIDAAMVEQNVAELFVRTLRHAGITRIWGLPGDSLNAFTDALRKDGGIEWLHARHEESAAFAAGAEAEITGELAVVAGSCGPGNLHFINGLFDAHRSRVPLLAIASHIPSAEIGSGYFQETLQRAADLLNDAKKVTILGGAGTEGAHEEVIALAKKLKAPIVHALRGKEFLEHDNPYDVGMTGLLGFSSGYRAMESCDALLMLGTDFPYRQFYPSKARILQVDVRGENLGRRTPIDLGLVGDVRDTAEALARIHAFVQAH",
    "response#3": "LKKLAQLLRYNDNIALMCGSGCAGAHDELVQLAATLKAPVVHALRGKEHVEYNNPYDVGMTGLIGFSSGFHTMMNADTLILLGTQFPYRAFYPTDAKIIQIDINPGSIGAHSKVDMALIGDIKATLAAIAA"
  },
  {
    "reference": "MLELTTQDIQLQQHFANKQAAIQGLAHALTAKGLVAEGYAQGMLNREAQHSTYLGNGIAIPHGTTDTRELVKQTGVTAMHFPQGLDWGDGNLVYVAIGIAAKSDEHLGILKQLTRVLSADGVEQALQQAKTAQQIIAIIKGEAQLTADFDASLIQLQFPASDMVQMSAVAGGLLKNTGCAENEFVADLVTKAPTHLGRGLWLVASDRAVKRTGMSIVTTANHCEYEQQAVKALIAFSVCNDVHQPLLNTITQCVFEQKQDQLLQADVQQLLNLFSGNAEQTIAQRTIAVGTITEETIAAETVAEPDSARAHTATFRIKNSHGLHARPGAMLVAEAKKFESNIRVSNLDGDGQVVNAKSLMKVIALGVKHNHQLQFTAEGPDAEAALQALGVAINAGLGEG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The ideal protein would be one that plays a critical role in phosphoenolpyruvate-dependent sugar phosphotransferase system through kinase activity, protein-phosphocysteine-sugar phosphotransferase activity within cytoplasm, plasma membrane.2. The Tele-phosphohistidine intermediate; for EIIA activity active site of the designed protein must be optimized for substrate binding and specificity.3. The protein must exhibit the following characteristics: The enzyme II FruAB PTS system is involved in fructose transport.; The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The designed protein sequence is ",
    "response#1": "KIQNFGRFLSNMVMPNIGAFIAWGFITALFIPTGWVPNETLASLVGPMITYLLPLLIGYTGGKLAGGERGAVVGAITTMGVIVGTDIPMFMGAMIVGPMGGWAIKAFDKKIDGKVRSGFEMLVNNFSAGIIGMLCAIIAFFLIGPFVKVLSGALAAGVNFLVTAHLLPLTSIFVEPAKILFLNNAINHGIFSPLGIQQASETGQSIFFLIEANPGPGLGILLAYMVFGKGTARQTAGGATIIHFFGGIHEIYFPYILMNPRLILAAIAGGMTGVFTLTVFNAGLVSPASPGSIFAVLLMTNKGSILGVVCSIFAAAAVSFTVAALLMKAQTSTEQAITACPTGVAHTFMAAEALEAEATRQGHQIKVETRGSVGAKNQLTEQEIAAADLVIIAADIDVPLDRFNGKKLYKTSTGLTIVTACPSGVANSIIAAGLLQQASKTLGWEAYIECHSTVIAGHTLSEEEINKADLVILAANGKIDMQRFVGKKVYQSPITACHLEQQRVWLTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPAAKARMAQLARGAISVTER",
    "response#2": "MMQKIQRFGSAMFVPVLLFAFAGIIVGISTLFKNKTLMGPLADPDGFWYQCWYIIEQGGWTVFNQMPLLFAIGIPVALAKKAQARACLEALTVYLTFNYFVSAILTVWGGAFGVDMNQEVGGTSGLTMIAGIKTLDTNIIGAIFISSIVVFLHNRYFDKKLPDFLGIFQGSTYIVMISFFIMIPIALAVSYIWPMVQSGIGSLQSFLVASGAVGVWIYTFLERILIPTGLHHFIYTPFIYGPAVAEGGIVTYWAQHLGEYSQSAKPLKELFPQGGFALHGNSKIFGIPGIALAFYVTAKKEKKKLVAGLLIPVTLTAIVAGITEPIEFTFLFISPFLFAVHAVLAATMSTVMYMAGVVGNMGGGLIEAVTLNWIPLFGSHGMTYVYQILIGLSFTAIYFFVFRFLILKFNIATPGREVLAKENIKLNQTVSSKEEAIKLAGQTLIDNGYVTEDYISKMFEREETSSTFMGNFIAIPHGTEEAKSEVLHSGISIIQIPEGVEYGEGNTAKVVFGIAGKNNEHLDILSNIAIICSEEENIERLISAKSEEDLIAIFNK",
    "response#3": "MITQIRVDDRLVHGQVAVVWTKELNAPLLVVANDEAAKNEITQMTLKMAVPNGMKLLIRSVEDSIKLFNDPRATDKRIFVIVNSVKDACAIAKEVPDLEAVNVANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQLVFS"
  },
  {
    "reference": "MEFKNVNVVKKANVYFDGKVTSRTVLFENGEKKTLGFMLTGEYEFNTGAEELMEVLNGEMDICVAGASDYTLYKEGQSFVVPANSSFKIIIKTFADYCCSYKENG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must be able to guanosine phosphorylase activity, pyrimidine-nucleoside phosphorylase activity, thymidine phosphorylase activity.2. The protein must exhibit the following characteristics: Catalyzes the phosphorolysis of diverse nucleosides, yielding D-ribose 1-phosphate and the respective free bases; Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates. The designed protein sequence is ",
    "response#1": "MDIRRVNIEGAD",
    "response#2": "MITK",
    "response#3": "MFLDHYFLRLPDSGD"
  },
  {
    "reference": "MSKSALLVLEDGTVFRGVSIGADGISVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHIGNTGTNSEDEESTAIHAQGLVIRDLPLIASNFRSEQSLSDYLKSQNIVGIADIDTRKLTRILREKGAQNGCIMAGDNLDEALALAKAKEFPGLKGMDLAKVVSTKEAYAWKQGSWTLEGGLPEAKADSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTSAEDVLALNPDGVFLSNGPGDPEPCTYAIEATRVFLEKNIPVFGICLGHQILALASGAKTVKMKFGHHGANHPVKDLDRGVVMITSQNHGFAADEATLPDNLRATHKSLFDGSLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIKQFRA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design must be focused on enhancing the efficiency of nitrogen compound metabolic process, arginine biosynthetic process. The designed protein sequence is ",
    "response#1": "YSMDANLLHISYEGKALENPWTEPSEDMFRLTVSPEAAPDKAQYIEVDFERGDAVAIDGEKLTPAALLAKLNEIGGRHGVGRLDLVENRYVGMKSRGVYETPGGTILQVAHRAVESLTLDREVMHLRDELMPRYAKLIYNGFWFAPERLMLQAAIDQTQQTVTGTARLKLYKGNVSVVGRKAAKSLYRMDYVTFEEDTVYDQHDAEGFIKLNALRLY",
    "response#2": "FGEKAAITAELQARGADVVVLPPTASLKAVAAYHPDGILLSNGPGDPTDYHTYLATIRQLAQRYPLAGICLGHQLIALAYGAQTYQLSFGHHGLNHPVQACADGRIIMTSQNHDYAVDPASIKGTPLIVTHTELNDGSIEGLRLPHQAVMSVQFHPEAHPGPQEAGQFFDDFL",
    "response#3": "FGEKAAITAELQARGADVVVLPPTASLKAVAAYHPDGILLSNGPGDPTDYHTYLATIRQLAQRYPLAGICLGHQLIALAYGAQTYQLSFGHHGLNHPVQACADGRIIMTSQNHDYAVDPASIKGTPLIVTHTELNDGSIEGLRLPHQAVMSVQFHPEAHPGPQEAGQFFDDFLG"
  },
  {
    "reference": "MIVREGSAEILVPEVYHGPGKRGTGFYNADQKINRDITIEFIKKMGIRNVLDGFGGTGIRGIRISKETDSAVTISEVSPDSYRLIRDNVERNGSQASVINDSFECVLQHGAYEYVDVDPYGSPVPYLDAALMGVKRNGFLGVTATDQSALTGSVPHKTRIRYDALIKNDTFRHEMGIRLLIGYMAKRAASLGRFIDPLISIWHGHYYRVFVRVRKGFEGAGRMMQNLGYVNKHNLLSGIYQDMDEGPVWKGNLQDNAVAEAVLASAGHKAFNPEENRLLFCDLTDIARARHTSLPDIESVIDALSSSGHAASRTMFSPTGIKTDASCDLVESLMMDTLHRRSPA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups..2. The protein contains novel Trm1 methyltransferase domains that confer a unique function or activity.3. We are looking for a protein with broad substrate specificity for guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) +   N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine.4. The protein must be able to tRNA (guanine-N2-)-methyltransferase activity, tRNA binding. The designed protein sequence is ",
    "response#1": "LDIVGDIAIVSIPDEILSEREVIVSAIRKLYPKVKVIARRGFHSGLYRIRELEVIWGENRLHTIHKENGVLIKVDLSKVFFNPRMKGERYRIAQLVNDGERILVPFAGVIPYPLVIARFKNVEVYAVEINEFAVKLAEENLELNRDRLKGKIKIIHGDVFEVLPNLPNFDRVVSPTPKGVDALSLTLSKAEKFLHYYDFVHESEIERFRERVLEECRRQGKECRVSVRKVSDYKPHVYKVCADVEILL",
    "response#2": "TIHKENGVLIKVDLSKVFFNPRMKGERYRIAQLVNDGERILVPFAGVIPYPLVIARFKNVEVYAVEINEFAVKLAEENLELNRDRLKGKIKIIHGDVFEVLPNLPNFDRVVSPTPKGVDALSLTLSKAEKFLHYYDFVHESEIERFRERVLEECRRQGKELDIVGDIAIVSIPDEILSEREVIVSAIRKLYPKVKVIARRGFHSGLYRIRELEVIWGENRLHTIHKENGVLIKVDLSKVFFNPRMKGERYRIAQLVNDGERILVPFAGVIPYPLVIARFKNVEVYAVEINEFAVKLAEENLELNRDRLKGKIKIIHGDVFEVLPNLPNFDRVVSPTPKGVDALSLTLSKAEKFLHYYDFVHESEIERFRERVLEECRRQGKECRVSVRKVSDYKPHVYKVCADVEILL",
    "response#3": "MFETVGHIAHLNLRDDHLPYKKLIAQVVLDKNKPKIQTVVNKIDAIQNDYRTMQLEVLAGIDSLVTTVIESGLRFQVDLSTVYWNSRLSTERQRLVDHVFKNSDVVCDVFSGVGPIAISAARKVKYVYANDLNPTAVEYLERNIVLNKLERKIEVFNMDARRFISSIYSSQHVHPVTQVVMNLPNDAAEFLDVFRGISWNHHLAVPSCVMPNIHVYGFSKAEDPEYDFHERINLTLGENVSDVEMHRVRLVAPGKWMLCASFTLPGREGGRKS"
  },
  {
    "reference": "MKIVVATHNEGKLVEIRRILEEDLGEAAATIELVSAGSLHLPDPVETGVTFQENALLKARDVASRTGLPAVADDSGLIVDVMGNAPGILSARWAGAHGHDKANNALLLAQIEDIPDGKRTGRFRCAAALVVPDVEAGADVTGGTSGAAAGTTMVQARYSIKSETVEVGEMPGVIIRKPRGEHGFGYDPLFVPDDQPGRTSAEPDYEGEPLTSAEMTPDEKNAISHRGKALKALVPAIEALL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The Mg(2+), substrate binding site should be stable and able to withstand changes in environmental conditions.2. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.3. The protein design must be able to perform metal ion binding, XTP diphosphatase activity, ITP diphosphatase activity, dITP diphosphatase activity in order to contribute to purine nucleoside triphosphate catabolic process, nucleotide metabolic process.4. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions. The designed protein sequence is ",
    "response#1": "MSKAPVLIASNNAGKLAELQAILAPWGIELKSQGELGVPEAEEPHRTFVENALAKARHASAATGLPALADDSGICVDALKGAPGIYSARYAGEPKSDARNNAKLLAELADVPDAQRGAHYVCVLALVRHADDPEPLIAEGRWHGRILHAPRGDGGFGYDPLFLVPSLGRTAAELAPEEKNAISHRGQAMRALLARLAAL",
    "response#2": "MLDRLVLATRNAHKVTELRAILGEALPGVELVSAGALGVDLDVPEDAATFAENALAKARAGVAHTGLPAVADDSGIEVDALGGAPGIFSARWAGRHGDDAANLRLLLAQLADVPDERRGAAFVCAAALALPDGTERVVEGRWPGRLARAPRGDGGFGYDPVLVPEGQTRTSAELTPTEKDALSHRGRAFRALAGLVRELLA",
    "response#3": "MNNEIIIATNNQGKIKEFNAMFADLPVTVKPQSDFDLEETGTTFVENAIIKARNAAKISGKPALADDSGLCVDALNGAPGVYSARYAGEHASDADNNAKLLKELQGVADRTAYFVCVLVALRAPNDPIPVTISGRWDGMILHEQHGENGFGYDPVFYFPELNKTAAELSKDEKNAISHRGNALKQLIKVLKQ"
  },
  {
    "reference": "MDLGSREAFDRTGTLGIEEEFFVVDEYGRPTAGTDELVYENEPPEPLKDRLDHELFKCVIETQTPTIGSLSAADDALAEVRKALVSHAETHGFGIAAAGLHPAAKWRELDHAEKPRYRAQLDRIQYPQHRNTTAGLHIHVGVDDADKAVWVANELRWYMPVMLALSANSPYWNGFDTGLQSARAKIFEALPNTGMPTAFEDFESFQSFERTMVETGSINDRGELWYDVRPHSEHGTVEVRTPDGQADPDHVLAFVEYTQALVEDLSARYEDGESGANHRRELLDENKWRALRHGHDAELLDRSLEESVPLGELVESECQRLGVSGIRDVYEAESGAEKQRRLLESEGMDALCGSLSVEWDSS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Catalyzes the synthesis of gamma-glutamylcysteine (gamma-GC), the main low-molecular-weight thiol compound instead of glutathione in halophilic archaea..2. The protein design must be able to perform ATP binding in order to contribute to cellular modified amino acid biosynthetic process.3. The desired protein should be able to perform ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-   cysteine + H(+) + phosphate The designed protein sequence is ",
    "response#1": "EKLFVMNFPQLMPPTLISRDLDEINAFRDKHGAVVMKPLHGHGGAAVFRVMPQDMNFGSLFDMFTVTFKEPWVIQQFIPEVKHGDKRIILVNGEFAGAVNRVPAADDLRSNMVRGGAAQETELTPREREICATVGPALRERGLLFVGIDVINGNLTEINVTSPTGIRAIARE",
    "response#2": "KLSDPASRQALKSMQRGIEREALRIEKSGHLALDKHPKALGSALTHSRITTDYSESLLEFITPVFADIDELVEDLTLTHAYSVRHLNGQRLWPVSMPCYLGDGADIPIADYGSSNSGQMKSLYRKGLTYRYGAQMQIISGVHFNFSVSDKLWHRLYELSDKSLSLDDFISDSYFGLIRNYRRLVWVLPYLFGASPALCSTFIQDPKTSNFPFEVIGDGTLFMPYATSLRMSDLGYTNQEQDNLNISYNSLTDYLTGMQAAINMPSANFAKIGVKVDGEYRQLNDNILQIENEFYSPIRAKRVAKGSEKPSESLARAGVEYIEVRALDVNPYSAVGIEKSQIRFLDLFLLNCLLQPSAPSDATEEAEIAENLQAAWLEKHPQALQGIQRGLERETLRVNADGTLATTGHPPALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDVHRYTARQLGDERMWPLSMPCYIAPGQDIELAQYGTSNVGRLKTLYREGLKNRYGALMQTISGVHYNFSLPMAFWQAKCGVEDAESGKEAISAGYFRSIRNYYRFGWVIPYLFGASPAICSSFLQGKPTTLPFEETGNGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLHEYVAGLKRAIKTPSEEYAKIGLQKDGKYLQINSNILQIENELYAPIRPKRVTRRGETPSDALLRGGIEYIEVRSLDINPFSPIGVDAQQVRFLDLFMVWCALADAPEMSSDELLCTRTNWNRPWCASDAALLD",
    "response#3": "KLFVMNFPQLMPPTLISRDLDEINAFRDKHGAVVMKPLHGHGGAAVFRVMPQDMNFGSLFDMFTVTFKEPWVIQQFIPEVKHGDKRIILVNGEFAGAVNRVPAADDLRSNMVRGGAAQETELTPREREICATVGPALRERGLLFVGIDVINGNLTEINVTSPTGIRAIARLGGPDVAAKVWDTIPC"
  },
  {
    "reference": "MSVFGKLFGAGGGKAGKGGPTPQEAIQRLRDTEEMLSKKQEFLEKKIEQELTAAKKHGTKNKRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGYAAKAMKAAHDNMDIDKVDELMQDIADQQELAEEISTAISKPVGFGEEFDEDELMAELEELEQEELDKNLLEISGPETVPLPNVPSVALPSKPAKKKEEEDDDMKELENWAGSM",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be designed to localize specifically to postsynaptic density, ESCRT III complex, plasma membrane, kinetochore microtubule in order to perform its role in regulation of centrosome duplication, late endosome to vacuole transport via multivesicular body sorting pathway, viral budding, plasma membrane repair, autophagy more effectively.2. Ensure the presence of a signal peptide within the protein.3. The protein must exhibit the following characteristics: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs; ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released; The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis; CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan.; When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding; Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase; ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. The designed protein sequence is ",
    "response#1": "MDPMDPNLDPWNHPGSQPTTPCNKCYCKKCCFHCQKCFLTKGLGISYGRKKRRQRRRPPQGDQAHQVPIPKQPSSQTRGDPTGPKESKKKVESKTETDPCD",
    "response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
    "response#3": "MEPREGEASAQEAPGGGAAGSAPQNPPPRRRPRRHSSSFSSSSSDSSSSSGSSSSGSDSDSSSSSSSSSSSSSSSSSSSTSSSSSEDEERPRRRRRRKRERPRAKGRERRSRRRRRSRSESSHSLSSSSSSPSPPPRRQKRRSQDRGGPRQRRAHSRERPCPRRKPDPGADGRRQRRGSRGRSPRREARGRPTRRSRSSYSSGSVSSASSARSSLSPPPRPTRRPAGPRPSSKRRAPARRSPSPRHPPPKPPPRRPAASPAPPRRGGRSRSRSPRQRARSPPPRTRRSPSPRRPPPRRSPPPRRSPPQRRSPRRRSPSPRRRSPPPRRSPLQRRSPRRPRSSPRRQSPPRRSPAPRRSPPQRRSPRRRSPSPRRRSPPRRSPPQRRSPQRRSPQRRSPQRRSPARRSPPQRSPPRRSPRRRSPPRRSPVQRRSPPQRSPAAQSPPPKPAPSPAPAAPPAATAQPQAPAAPAAQTAPAAPAATPAAPAAPAAPAAPAAPAAPAAPAAPAAPATPSAAAAEPAAS"
  },
  {
    "reference": "MDKLNSTPQPAEEPAIGVQKIRTMIEGFDDITHGGLPAGRATIVSGTSGTGKTMLAVQFVYNGISNFNEPGIFVTFEESPSDIIKNAYSFGWDLQKLINEGKLFVLDASPDPEGQEVVGHFDLSALIERIQYAIQKYKAKRVSIDSVTAVFQQYDAASVVRREIFRLVARLKQLGVTTIMTTERVEEYGPVARYGVEEFVSDNVVIVRNVLEGERRRRTMEILKLRGTTHMKGEYPFTITNNGINIFPLGAMRLTQRSSNIRVSSGVKTLDEMCGGGFFKDSIILATGGTGTGKTLLVSKFLQEGCMSGERAILFAFEESRAQLSRNASSWGINFEELERKGLLKILCTYPESAGLEDHLQTIKSEIAEFQPSRIAIDSLSALDRGASNNAFRQFVIGITGYAKQEEITGFFTNTTNQFMGSHSITESHISTITDTIILLQYVEIRGEMSRAINVFKMRGSWHDKGIREYTISKEGPEIKDSFRNYEGIISGSPSRISIDEKSELSRIVKGVRDKIGE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Binds to DNA2. The protein should have a high affinity for ATP.3. The protein must be able to protein serine/threonine kinase activity, DNA binding, protein serine kinase activity. The designed protein sequence is ",
    "response#1": "PTRCQVLGRGAYGTVFKAIYRDRSVAVKIIRAQAASTLHNESHLLNLEHRNIVRLLKLESAADFGLVIMECPRGQSLQRIVDTLALPLMHRVLITLDVVAALRYCHSQNVLHLDVKPTNILVALGTKSSITCNSSKIKVKRSYICKLCDFGSSIEMGEFCAWQEPSVAKGTLRYMSPEALRSDTLTEASDIYSLGITMWQLQARRLPYHTLDCNETIAYQVVKHELRPDNYHQLKILALDSPIDCDWDLAHESTANVICRRANTSARRNLSLDPSYTVGRDLKKKRHRNRLALHFDSPAPEGSACSESAYSQLYKSCWVSAPELRLSSIQLKHELEFL",
    "response#2": "KEMPIYAGSGSYGVVKIFKKTDIAVKKVLECFKTELLMTLIAGECALRAKSTLRINNIIPLLAFSIPSKELVFPAYHMDMDSYYHRLARIDKTVQHWKAIEKTFMDLGKAVVFLNVSCGLTHLDIKCGNIFVNVTEGPNPILVDAVIGDFSLALLNTNSTILKSRFDVNISSDKIQSLKVCRGNIKPVFDLVLGHGQTQPCELMIKALNGVGFERRSTPLTSDEGVSIDMYALGQSLMEVILAAGMNFTHRFGISSNPLHFYYHRLMRADYLLDILAYRCMLYQHLFPMTPLTSKNGIPWERAEKIRLQLHSSRHRAEFDKYLEAYDITHRKLFDSLNIFPYR",
    "response#3": "KLNENHSGELWKGRWQGNDIVVKVLKVRDWSTRKSRDFNEECPRLRIFSHPNVLPVLGACQSPPAPHPTLITHWMPYGSLYNVLHEGTNFVVDQSQAVKFALDMARGMAFLHTLEPLIPRHALNSRSVMIDEDMTARISMADVKFSFQCPGRMYAPAWVAPEALQKKPEDTNRRSADMWSFAVLLWELVTREVPFADLSNMEIGMKVALEGLRPTIPPGISPHVCKLMKICMNEDPAKRPKFDMIVPILEKL"
  },
  {
    "reference": "TICYNHLTIRSEVTEICIICDDDYYF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Antagonist of muscle and neuronal nicotinic acetylcholine receptors (nAChR) with highest affinity for neuronal alpha-7/CHRNA7 nAChRs.2. The protein should be optimized for extracellular region-specific folding and stability, while maintaining toxin activity. The designed protein sequence is ",
    "response#1": "ETRAD",
    "response#2": "MKIHIVQKGDTLWKIAKKYGVNFEELKKMNTQL",
    "response#3": "MEQLLPS"
  },
  {
    "reference": "MVNSQPPTENRKSRVLVAVGGGIAAYKVCELVSSLFKSGGEVRVIVTRSAQQFITPLTLATLSRHPAYTDDDFWQPTHSRPLHIELGEWADLMVIAPLTANTLAKLTHGMADNLLTNTVLASTCPVLLAPAMNTDMWEQLTVQRNWQQLLTDSRYHGIGTASGLLACDRVGAGRMAEPPEILTYTQSLLHTKGKRDLTLKRVLISAGGTQEYLDPVRFIGNPSTGKMGLALAQAALHRGAQVTLVHGAANWDVPLGVQAIPIISAEEMQQVMLEYLPNADIIIMSAAVADVKPKDYSTEKLPKRSLPQSLPLEPVPDIVAQLGQLKQPHQRLIGFAAQTGDIVTPAKEKLYSKKLDAIVANPIDKPDSGFGSNNNQAIFLNSQGQQQTIAPCSKLQMAHYLFDFIFK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be optimized for phosphopantothenoylcysteine decarboxylase activity, FMN binding activity in order to improve coenzyme A biosynthetic process, pantothenate catabolic process.2. The Proton donor of the protein must be engineered to enable effective catalysis of H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2. The designed protein sequence is ",
    "response#1": "LQGKKVIVTAGGTREAIDPVRFIGNRSSGKMGFALAEAAWLRGADVVLISGPVGLAPPFGVTCHYVESAAQMRQLVLDHYEEADIVVKAAAVADYRVANPGTQKIKKTDNDLELLLTKNPDILKELGEKKRHQVLIGFAAETEELLANAQEKLVKKNLDLIVANDVTRQDAGFNADTNEVTLL",
    "response#2": "KSLLLIIGGSIAAYKALDLIRRLQERGAHLNIIMTKAAQKFITPLAAEALSGHTVYSDLFSREEEHDIGHIRLAQGADLIILAPATANRIAKIANGIGDDLADCVLLAARCPLLIAPAMNPAMWAHPATVRNVAQLRANGVHIIGPEAGKMAERDETGYGRMSEPLTIVAASEIGRRVEDILAPQGDLGWQVVVSAGGTREAIDPVRYLGNRSSGRMGNAVAEAAAQRGAEVTLVTTAGAVEQRRVTVVVVDTAEEMAAAVWKAAAEADVAVLAAAVADFRPADPAAGKLRRADGPPHIVLEPTPDILAGVAKMSPRPFLVGFAAETGSLDEAVVKARRKGVDLLVANDVTRAGSGFGTDTNEVALICRDGSVEELPLMSKRRLANRLLDRVIALLE",
    "response#3": "LQGKKVIVTAGGTREAIDPVRFIGNRSSGKMGFALAEAAWLRGADVVLISGPVGLAPPFGVTCHYVESAAQMRQLVLDHYEEADIVVKAAAVADYRVANPGTQKIKKTDNDLELLLTKNPDILKELGEKKRHQVLIGFAAETEELLANAQEKLVKKNLDLIVANDVTRQDAGFNADTNEVTLL"
  },
  {
    "reference": "MVNLSDGLGSPLYLTGPILARYPHDMNAPPLSATPADLSARRPATAVILAAGMGTRMKSAQPKVVHPLAGRPMLRYLLDHAATVFGRIVVVVGPGMEQVAALAGPHQAVVQADRLGTAHAAAQAADLFGDGDVAVLYGDNPLITPESMRNLLACRATPGVGLALMAMRPRDPGRYGRIVSEDGLVKRIVEWADASAQERAIGLCNAGVLCAEAATFRRWLSMVRNDNAQGEYYLGDVVALAVAEGEQVRAVEAPEDELRGINSRAELAEAEACVQKRLRAAAMDAGVTLVAPETVFLSADTVFEQDVVVQPHVVFGPGVRVHRGAEIRAFSHLEGCVVGPEALIGPYARLRPGSDVGAAAHVGNFVELKATTLGAGAKANHLTYLGDAAIGAGTNVGAGTITCNYDGVFKHRTEIGTDCFIGSDAILVAPVSIGDGALVAAGSVVTEDVPAGALAFGRARQENKDGRGAALRDALRRKKKEQG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc)3. The designed protein have MobA-like NTP transferase domains with specific sequence motifs that are crucial for its activity.4. The designed protein must possess glucosamine-1-phosphate N-acetyltransferase activity, magnesium ion binding to support cell morphogenesis, cell wall organization, regulation of cell shape, lipid A biosynthetic process, UDP-N-acetylglucosamine biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "MTALILAAGKGTRMPSPRPKVLQTLVGESMLGLVYAALAALPGVERVLTLVGYEADLVRAEAERCARRGPRPDEAANCIEQVEQRGTGHALMTALPELENGCGRVLVVNGDTPLLPSAALRRFVTEAAGADLAFATFVTDDPTGYGRLLRRPDGAVTAIVEHKDATDEQSAIREINSGVYAFDVAALRSALGRLSSDNAQGEMYLTDVLGIVRADGHPVRAHVATDHLLVEGVNDRVQLAALGAELNRRIVTAHQRASRLGDGVRVGPFANLRPGTVVGNGTKIGDFVEVGRAQLAACEAVFQARMRRAAMDAGVTLQAPEMVFFSHDTVLEADVFVEPNVVFGPGVHVEGGTRIRAFSHLEGAHVGRDATVGPFARLRPGAELAADTHIGNFVEIKNATVAEGAKVNHLTYIGDADIGAKSNIGAGTITCNYDGYLKHRTTIGAGCFVGSNATLVAPVALGDGAYVAAGSVVTDAVPADALAIGRGRQRNIEGWVARKRAGTPAAEAAEAALQGAPEPAVTEPAAGESS",
    "response#2": "AIVLAAGKGTRMKSDLHKVLHPLAGRPMLLHLLDNVAALAPARTVVVVGAGRAQVEAAVAPLGAEVVHQTEQLGTAHAVRQAETALGGFAGDVLVLYGDVPLIGTATMREMLDRLHAADRPAAVLLTVTLDDPTGYGRVVRDANGAVTAIVEHKDASAEQLAIEEVNTSVYVFDAAVLRDALGRLGRDNAQGEMYLTDVLGLVRDAGHRVGAAIAGDWTETLGVNDRVQLAEARRILNDRLLTDHMRASRLGDGVRVGPFANLRPGTVVGNGTKIGDFVEVEGVTVVDPGSTWVDVSVTFEPDVTVHPFTELRGATHLATGVEVGPQSRLTDTSVGAGAVVSFTVAEGARIGAGASVGPYAYLRPGTDLGPKSKAGTYVEMKNASIGEGTKVPHLSYVGDATIGEFTNIGAASVFVNYDGEAKHHTTVGSHCKTGSDNMFVAPVTVGDGAYTAAGSVITKDVPPGSLAVARGQQRNIEGWVQRKRQGTPSAEAAERALAARTKAEQEPDHE",
    "response#3": "VIVLAAGAGTRMKSRTPKILHPVGGISMLGHALAAASALHPRFLAVVVRHERDIVAAHAAGHAPAAVIVDQDEVPGTGRAVQVAVSALDAVSTLEGTVVVTYGDAPLLEADTLRSLVAVHETDGNAVTVLTASVLGTDGYGRIVRDGGRVVGIVEQKDASPEQLAITEINSGTYAFDAAVLTEALGRLSTDNAQGELYLTDVLGIARADGRPVGALVAADHLQTEGVNDRVQLAELARELNRRTVAAHQRASRLGDGVRVGPFANLRPGTVVGNGTKIGDFVEVGPDTQLGRDVLIEPNVFFGPGVRVADNVKIRANSHIEGVTLIDPSSTYIGDDVIIGKDTIIYPGNVIEGNTIIGERVMLYPNSRIKDSQIDEEVEVQSSVILDSKIGANTTVGPFAYIRPESVIGKSARIGDFVEIKKSTIGDNTKVSHLTYIGDAEVGSACNFGCGTVVVNYDGKDKYKTIIGDNSFIGCNTNLVSPVKVGDNTYIAAGSTITNEVKEGDLAIARAKRAQLAACEAVFQARMRRAAMDAGVTLQAPEMVFFSHDTVLEADVFVEPNVVFGPGVHVEGGTRIRAFSHLEGAHVGRDATVGPFARLRPGAELAADTHIGNFVEIKNATVAEGAKVNHLTYIGDADIGAKSNIGAGTITCNYDGYLKHRTTIGAGCFVGSNATLVAPVALGDGAYVAAGSVVTDAVPADALAIGRGRQRNIDGWVIRKRPGSPAAEAAERALAAGDTDRARELAPGEGTGGAQ"
  },
  {
    "reference": "MIFSISKRKLICGFSLVALTIAGIVGGVYLVTKNNQQTTPQTNHFNVADPVNKVPNWRKLGPETQRELRDLLYPLDDTGYFIYKYGAISRYLQSQKELDELVDYRTVLPSTQKHFKYDSFNQSVLESKLRKWLMKAIKQHPYFQHFEFDPVLKAQYNINIPAQKITVNAVWFYKKDNDLTTGKPIRYWDQFEIKLKQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization.2. The designed protein should have a specific function within the membrane. The designed protein sequence is ",
    "response#1": "KWNNISTSFVHSLITGVWSVLCFCMHPQMAEDLIETHSVFSHALVSVSIGYFIYDFLDMVINQKIIHSWELLFHHVVVITCFGISVLTCRYVGFAVVALLVEINSVFLHLRQVLRMANLAKSTFYRVNSMINLGTYVVFRINTLAWMTRWLVLNRDLIPLFSYTIGSVGLAIMTAMNIVLFYRLMN",
    "response#2": "KWNNISTSFVHSLITGVWSVLCFCMHPQMAEDLIETHSVFSHALVSVSIGYFIYDFLDMVINQKIIHSWELLFHHVVVITCFGISVLTCRYVGFAVVALLVEINSVFLHLRQVLRMANLAKSTFYRVNSMINLGTYVVFRINTLAWMTRWLVLNRDLIPLFSYTIGSVGLAIMTAMNIVLFYRLMY",
    "response#3": "KWNNISTSFVHSLITGVWSVLCFCMHPQMAEDLIETHSVFSHALVSVSIGYFIYDFLDMVINQKIIHSWELLFHHVVVITCFGISVLTCRYVGFAVVALLVEINSVFLHLRQVLRMANLAKSTFYRVNSMINLGTYVVFRINTLAWMTRWLVLNRDLIPLFSYTIGSVGLAIMTAMNIVLFYRLMY"
  },
  {
    "reference": "MKLLNWQNVRKRWKKLALISLPIGYGLYALKRNYEISKYMRAVCNDVKDKDSGGSSKNVLVILNPMADKGKSEKNFTKFCEPVLHLSGYTVDIKKTKHIGHAKTLVESLENIPDVIVVAGGDGTSSEVVTGLLRRGDTPCPIVLLPLGEKSKTALRYLDHTPNSKLETANRLVSSLQPLIEDKTQFCSVIKYDIIENEENQVNKPIYGLQKFSWGLLRDIEANKNKYWYFGNLRHHAAALFNAFSSKLEWNIAAKVVLTPPCPGCTNCKKPINQKPKISFASFYTTRQVTEKTENFINELCDVEESENIEMNQIDIFCNKNSESFFELKTDLVKSLTPGFNFIENISNVVHYAIEPATSLKSRTIKLYPSLTTANTYYIDGEEYEVRPIKISLIPNAIKLFC",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that can perform metal ion binding, kinase activity in mitochondrial intermembrane space, mitochondrial inner membrane to support glycerolipid metabolic process.2. The protein contains novel DAGKc domains that confer a unique function or activity.3. A protein should be able to manipulate the activity of enzymes in the Lipid metabolism, glycerolipid metabolism pathway, either by activating or inhibiting their function.4. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.5. We are looking for a protein with broad substrate specificity for an N-acylsphing-4-enine + ATP = ADP + an N-acylsphing-4-enine   1-phosphate + H(+). The designed protein sequence is ",
    "response#1": "LAGLEWGAFRDTNARRDKYWYFGGLRGYAAYIFAGFKGEKTITWNVQANMEYSPPCNGCSNCYEQRLDLHPDQAQSKSWWSAFGKRQPGSVVPTVDYRGKVNEDCRTVRKLDVATSDFSLISSNVSKEHNKVPQLQVSFGPEHVSYNDFVKEGWRRENGEDPSRPCKILAKEVKLWPVQAVKHQDNATASSTQDNIPTGPKDIEKWISIDNEDYEVKAVHATLLPNLVKMFYSPK",
    "response#2": "LNGFSWGLLKDIDSTKEKYWYFGPIRHYVAAFSKLFSKNWSLVTDYVYTPPCSGCADCFEIPKDESTVLKQSFFGKLVNSNRRNVKSQKQLIKNSECSNELNGKMEANQIDITCIQNNEDFSELETNFISSLQPGWNFIKTIPSITCSSIVPQLVLRSRTIQLYPNGESTNDSYSIDGEEYDARPIKLSVVPNAIKVFLK",
    "response#3": "LAGLEWGAFRDTNARRDKYWYFGGLRGYAAYIFAGFKGEKTITWNVQANMEYSPPCNGCSNCYEQRLDLHPDQAQSKSWWSAFGKRQPGSVVPTVDYRGKVNEDCRTVRKLDVATSDFSLISSNVSKEHNKVPQLQVSFGPEHVSYNDFVKEGWRRENGEDPSRPCKILAKEVKLWPVQAVKHQDNATASSTQDNIPTGPKDIEKWISIDNEDYEVKAVHATLLPNLVKMFYSPN"
  },
  {
    "reference": "MSASQNIVVSETTMSSIIPNNNNNNNNSSSQKLPPCLISISKKKLLKNIDIINGGGQRINAWVDSMRASSPTHLKSLPSSISTQQQLNSWIMQHPSALEKFEQIMEASRGKQIVMFLDYDGTLSPIVDDPDKAFMSSKMRRTVKKLAKCFPTAIVTGRCIDKVYNFVKLAELYYAGSHGMDIKGPAKGFSRHKRVKQSLLYQPANDYLPMIDEVYRQLLEKTKSTPGAKVENHKFCASVHFRCVDEKKWSELVLQVRSVLKKFPTLQLTQGRKVFEIRPMIEWDKGKALEFLLESLGFGNTNNVFPVYIGDDRTDEDAFKMLRDRGEGFGILVSKFPKDTDASYSLQDPSEVMDFLRRLVEWKQMQPRM",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Removes the phosphate from trehalose 6-phosphate to produce free trehalose; Trehalose accumulation in plant may improve abiotic stress tolerance ..2. A protein that is capable of catalyzing reactions within the Glycan biosynthesis, trehalose biosynthesis pathway with high efficiency and specificity.3. The protein must be engineered to have a cooperative binding mechanism with a divalent metal cation to ensure proper regulation.4. We need a protein that can perform alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-   trehalose + phosphate with high efficiency.5. A protein with trehalose-phosphatase activity is needed to enhance trehalose biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "MNELGWPAREYIVQIARFDPSKGIPNVIDSYARFRKLCVDKVMEDDIPQLLLCGHGAVDDPDASIIYDQVLQLIHAKYKEYAPDIVVMRCPPSDQLLNTLMANAKFALQLSTREGFEVKVSEALHAGKPVIACRTGGIPLQIEHGKSGYLCEPGDNAAVAQHMLDLYTDEDLYDTMSEYARLFPGAR",
    "response#2": "MNELGWPAREYIVQIARFDPSKGIPNVIDSYARFRKLCVDKVMEDDIPQLLLCGHGAVDDPDASIIYDQVLQLIHAKYKEYAPDIVVMRCPPSDQLLNTLMANAKFALQLSTREGFEVKVSEALHAGKPVIACRTGGIPLQIEHGKSGYLCEPGDNAAVAQHMLDLYTDEDLYDTMSEYARVGEIARMLGCLYRSLTALRRSTPDLTKLGFEDTQVAFDEDARWLRFRRGGVQVLLNFSEQPVSLDGAGTALLLATDDAVRLEGERAELGPLSAAV",
    "response#3": "MNELGWPAREYIVQIARFDPSKGIPNVIDSYARFRKLCVDKVMEDDIPQLLLCGHGAVDDPDASIIYDQVLQLIHAKYKEYAPDIVVMRCPPSDQLLNTLMANAKFALQLSTREGFEVKVSEALHAGKPVIACRTGGIPLQIEHGKSGYLCEPGDNAAVAQHMLDLYTDEDLYDTMSEYARRAYLQRSALTLKGLTYSPTGALLAASTTSLPETPRGERNWDYRYAWIRDSTFALWGLYTLGLDREADDFFAFIADVSGANNNERHPLQVMYGVGGERSLVEAELHHLSGYDHARPVRIGNGAYNQRQHDIWGSILDSFYLHAKSREQVPENLWPVLKRQVEEAIKHWREPDRGIWEVRGEPQHFTSSKVMCWVALDRGAKLAERQGEKSYAQQWRAIADEIKADILEHGVDSRGVFTQRYGDEALDASLLLVVLTRFLPPDDPRVRNTVLAIADELTEDGLVLRYRVHETDDGLSGEEGTFTICSFWLVSALVEIGEVGRAKRLCERLLSFASPLLLYAEEIEPRSGRHLGNFPQAFTHLALINALG"
  },
  {
    "reference": "YRTIDRIYPALTREHKLLIPDIKGEAHIVYEEGRLYPHHNLYYITSEIWDLRALQAVLLSGIARLFVSVYSTKMHGGFLRFQAQYLRRIRVPNWSQVPAAVRQELITAGAKPDLAACNRAVFALYAMTAEERAALGGNGD",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be optimized for site-specific DNA-methyltransferase (adenine-specific) activity activity in order to improve DNA restriction-modification system.2. The protein should have a low Km value for a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an   N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-   homocysteine.3. For general function, the protein need meet that A methylase, recognizes the double-stranded sequence 5'- CTCGAG-3', methylates A-5 on both strands, and protects the DNA from cleavage by the AbrI endonuclease. The designed protein sequence is ",
    "response#1": "IDMIFADPPYFLSNDGLTFKNSIIQSVNKGEWDKNDNEASIYNFNHEWIAQARQLLKDNGTIWISGTHHNIFTVGQVLKENNFKILNIITWEKPNPPPNFSCRYFTYSSEWIIWARKHSKIPHYFNYDLMKKLNGDKQQKDIWRLPAVGSWEKTQGKHPTQKPLGLLSRIILSSTQKDDLILDPFSGSGTTGIAGVLLDRNYIGIEQELEFLELSE",
    "response#2": "TVEGKWTKATLSIDELQLIKRVIAHPDVRKFKDIAKVDVGRYCDGANNYFLVDNETVKLYKLERFAHPMFGRSQHCPGIIYDEKQHIENQEKGLPTNFLYIDEEFEYLSKSVKNYIKLGEVEEYHKRYKCRIRKPWFKVPSVYSTEIGMLKRCHDAPRLIHNRVRAYTTDTAYRVSSTVTSTENLVCSFLNPITVITAELEGLFYGGGVLELVPSEIEKLYILIVEGLEHNVEELNLLIKDGQIERVIRQQGSLILGTLGFTQEENEKLVEIGRSLEIEGYVS",
    "response#3": "TVEGKWTKATLSIDELQLIKRVIAHPDVRKFKDIAKVDVGRYCDGANNYFLVDNETVKLYKLERFAHPMFGRSQHCPGIIYDEKQHIENQEKGLPTNFLYIDEEFEYLSKSVKNYIKLGEVEEYHKRYKCRIRKPWFKVPSVYSTEIGMLKRCHDAPRLIHNRVRAYTTDTAYRVSSTVTSTENLVCSFLNPITVITAELEGLFYGGGVLELVPSEIEKLYILIVEGLEHNVEELNLLIKDGQIERVIRQQGSLILGTLGFTQEENEKLVEIGRSLEIEGYVVE"
  },
  {
    "reference": "MARAFLFVLDSFGIGGAADAERYGDAGANTLAHIAEACAEGRADRERLRQGPLFVPHMASLGLGKAAETATGLGFAHFGTDLIANAFHGAAQEVSSGKDTPSGHWEIAGLPVRFDWGYFPDTVPAFPADLTAAMIREGEVPGILGNCHAPGTEIIERFGEEHIRTGKPICYTSVDSVLQIAAHEVHFGLDRLYEFCQVVRRLVDPLRIGRVIARPFVGETAATFQRTYNRHDYAVPPPEPTLLDRLTARGSRVIAVGKIGDIFAHRGISEVRKAAGNMAMFDKALGAMDDAGDGDLVFANFVDFDTEFGHRRDVAGYAAALEAFDRRLPEAFARLRQGDLLILTADHGNDPTWRGTDHTRERIPVIGMGPGLAGGDIGLRTTFADIGETVAEHLGLAPGRHGTSFHAMIGGHA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The Mn(2+) binding site should be located in a region of the protein that is accessible to the ligand.2. The protein should be designed to have high stability and activity towards 2-deoxy-alpha-D-ribose 1-phosphate = 2-deoxy-D-ribose 5-   phosphate.3. The designed protein must possess manganese ion binding, phosphopentomutase activity to support 5-phosphoribose 1-diphosphate biosynthetic process under certain conditions.4. The protein design should consider the endogenous proteins and enzymes present in the 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, Metabolic intermediate biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MRALIVVMDSVGIGGAPDAAGYGDEGADTVGHIAEACAAGQADSDQRQGPLQLPHLAALGLGEACEHATGRRPPGLQQKAGAGAAFGCASERSRGKDTPSGHWEIAGVPVPFEWGYFPKTVPAFPPDLIDQLCASAGLTGILGNRHASGTDIISDLGEAHLQTGQPICYTSADSVFQIAAHEEAFGLDRLYRLCEATRPLVDRFNIGRVIARPFVGRSASGFRRTANRRDYAVPPPEPTILDLATAERRHVVTIGKIADIFAHRGTGHNLKGDSNDTLFTRMLEGLDALDDGGLLFANFIDFDTLYGHRRDVAGYASALEAFDARLPELLDALRDDDLLVITADHGCDPSWSGSDHTRERVPVLIVNGRSAGSIGARTSFADTGATIAQHLQLTTRSL",
    "response#2": "MRALIVVMDSVGIGGAPDAAGYGDEGADTVGHIAEACAAGQADSDQRQGPLQLPHLAALGLGEACEHATGRRPPGLQQKAGAGAAFGCASERSRGKDTPSGHWEIAGVPVPFEWGYFPKTVPAFPPDLIDQLCASAGLTGILGNRHASGTDIISDLGEAHLQTGQPICYTSADSVFQIAAHEEAFGLDRLYRLCEATRPLVDRFNIGRVIARPFVGRSASGFRRTANRRDYAVPPPEPTILDLATAERRHVVTIGKIADIFAHRGTGHNLKGDSNDTLFTRMLEGLDALDDGGLLFANFIDFDTLYGHRRDVAGYASALEAFDARLPELLDALRDDDLLVITADHGCDPSWSGSDHTRERVPVLIVNGRSAGSIGARTSFADTGATIAQHLQLN",
    "response#3": "MRALIVVMDSVGIGGAPDAAGYGDEGADTVGHIAEACAAGQADSDQRQGPLQLPHLAALGLGEACEHATGRRPPGLQQKAGAGAAFGCASERSRGKDTPSGHWEIAGVPVPFEWGYFPKTVPAFPPDLIDQLCASAGLTGILGNRHASGTDIISDLGEAHLQTGQPICYTSADSVFQIAAHEEAFGLDRLYRLCEATRPLVDRFNIGRVIARPFVGRSASGFRRTANRRDYAVPPPEPTILDLATAERRHVVTIGKIADIFAHRGTGHNLKGDSNDTLFTRMLEGLDALDDGGLLFANFIDFDTLYGHRRDVAGYASALEAFDARLPELLDALRDDDLLVITADHGCDPSWSGSDHTRERVPVLIVNGRSAGSIGARTSFADTGATIAQHLQLHAR"
  },
  {
    "reference": "MTRSRRPVPCRTPAPTWGGSGREREGGQVTPTTAPRVAPPTPARRPGHPARPPGRDWRRQVPRTFAALLWFIAAICALAAVSGAANAEFQPIRTTIDTLLVPAPANLAYAVFLGTLAAAVLRRKRLAYWVLVVYFILSLVVGVLTGLLLVTVGADQLTDDAGRRLFTLVETVGVWVGIGFAAVALALLRAARREFYARVRSGSTRRALGVFLGLAAAAVGLGYLLLTAVPGSLHSWLDRVGYAIEKVFGGAITLDVTRQGQAPGWVNLLLGGMGAVAFLAGLFTLLRSQRAAAVLHADEEQRIRELLARYGDRDSLGYFATRRDKAAIFSASGKAALTYRVVNGVSLASGDPVGDPEAWGPAITAWLDQAREYAWTPAVMGASEAGARAYHRHGLKVLQLGDEAILLAREFDLGGRDMRPVRQAVHRVERAGYTAGVRRHADIPPAELADLAALATRWRDTEHERGFSMALGRLGDPADGDCVLVEARDGTGRVRALLSLSPWGGNGLSLDLMRRDRTAENGVTEFMVAALLGAAPRLGVERVSLNFAVFRSVFEQGARIGAGPVIRLWRRTLLFFSRWWQLESLYLSNAKYQPHWTPRYLCFGERRELARVGLAAAVAEGFVALPGGRPSPLYGAPPAGGGVGFVPPDAATVHAVSPEPAHEQLPEQVRVRRAKLDRLRAQGVDPYPVGWPRTDDCAAVRARHAGLAPDTGTGETVSVAGRVLLVRDHGRVCFVTVRDGSGDLQLVLEKELDRWRATVDIGDHVGAT",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Mg(2+) is required for the proper folding and stability of the protein.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. The designed protein should be involved in response to antibiotic, lipid metabolic process by localizing to plasma membrane, cytoplasm and exhibiting nucleic acid binding, lysyltransferase activity, magnesium ion binding.4. The protein must exhibit the following characteristics: Catalyzes the production of L-lysyl-tRNA(Lys)transfer and the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge. The designed protein sequence is ",
    "response#1": "MGASEDGAKAFARSGLSALQLGDEAILHVKDFDLDGREMRVTRQAVNRVERTGATFRVRRHSELSDAEMQEVIHRADAWRDTETERGFSMALDRLGDPEDGDCLLAEAFDGDGNMMALLSFVPWGKDGISLDVMRRDRSAPNGVMEYMVAQLCAHAGQIGVRRISLNFAVFRSAFEEGARIGAGPVLKVWRKLLLFFSKWWQLEALYRSNAKYAPEWYPRFLCSRVPEGVAVFFGLLGLLCLLLALVPPLRALLRPLVRYLDLLIVPVSANLAYAVFLFLLAAATAARKKVAWWLVVVYLGLLALVDVLGVVAGLYLESVASLVICGCALALLIVARREFYAASRRAAVRRALAVLAAGLVLGVLLGWGLVALFPGTLPDTQWLGWATDRVLGGLVSERAFDGRPPHALFFLLGLFGALALLNAAAALLA",
    "response#2": "MGASEDGAKAFARSGLSALQLGDEAILHVKDFDLDGREMRVTRQAVNRVERTGATFRVRRHSELSDAEMQEVIHRADAWRDTETERGFSMALDRLGDPEDGDCLLAEAFDGDGNMMALLSFVPWGKDGISLDVMRRDRSAPNGVMEYMVAQLCAHAGQIGVRRISLNFAVFRSAFEEGARIGAGPVLKVWRKLLLFFSKWWQLEALYRSNAKYAPEWYPRFLCARVPGAFAMFFALLGVYCAVIALIAPLRRVLSPVSAFLDLVAVPVSANLAYAVFLLLLAAAIAARKKAAWWLVVAYLSLLVLTDVLTLALLRDWSWIPNLVLCLIALGVLSAARGEFYALTRRGALRHALLVLVLGLALAVLVGWGLVELFPNDLPQGQRLLWAANRVLGGLVSNAQFDGTPPRALFFLLGLFGALALLNSARTLRLTWVPEAFGTFFGALALLCALLSFIAPLRVLLHPVVYFLDAVVVPVSPNLAYAVFLFLLGGATAARKKVAWWLLVIYFGLLALVDVLAVAFGFDWYSFSSLLVCGLALTLLIVARREFYAASRRAAVRRALAVLLIGLVVAILAGWGLVSLFPGTLPHGQHLVWAADRVCGGLVPVHTYAGRPPRSVAFLLGLFGALALLNAAATLLYNLPTWLARALLGAYGRSDSLGYFATRRDKAVVFAPNGRACVTYRVEAGVCLASGDPVGDPAAWTPAIDAWLAVARRHGWQPAVMGASEDGATAYARSGLSALQLGDEAILHVAHFDLDGRDMRVTRQAVSRVRRAGATTSIRRHSALSDEEMQRIIDRADTWRDTETERGFSMALDRLGDPADGDCLLVEAFDADGELIALLSFVPWGRDGISLDLMRRDRNAPNGVMEFMVAQLCAAAPGLGVRRISLNFAVFRSAFEEGGRIGAGPVLKLWRRLLLFFSRWWQLEALYRSNVKYGPEWYPRFLC",
    "response#3": "MGASEDGAKAFARSGLSALQLGDEAILHVKDFDLDGREMRVTRQAVNRVERTGATFRVRRHSELSDAEMQEVIHRADAWRDTETERGFSMALDRLGDPEDGDCLLAEAFDGDGNMMALLSFVPWGKDGISLDVMRRDRSAPNGVMEYMVAQLCAHAGQIGVRRISLNFAVFRSAFEEGARIGAGPVLKVWRKLLLFFSKWWQLEALYRSNAKYAPEWYPRFLCSRVPEGVAVFFGLLGLLCLLLALVPPLRALLRPLVRYLDLLIVPVSANLAYAVFLFLLAAATAARKKVAWWLVVVYLGLLALVDVLGVVAGLYLESVASLVICGCALALLIVARREFYAASRRAAVRRALAVLAAGLVLGVLLGWGLVALFPGTLPDTQWLGWATDRVLGGLVSERAFDGRPPHALFFLLGLFGALALLNAAAALLASHRTLLRDFGDHDSLGYFATRRDKAVVWDTGDPATATAGVSYRVVGSVSLASGNPLGDPARWPAAIEAWRRLARSGGWSLAVMGAGREGAEAYAGIGLTAYEIGDEAVLDLGTFSLSKPGLKAVRQSVARLQRRGYTTVVCRHGALTPADFEAMNTDAAQWRGDGGDERGFSMALGRLGDPLDDECVMVQARDGEGRLRGFLSFVPWGRNGLSLDLMRRDPSADNGLVELMVAALAERSATLGAGRVSLNFAMFREAFERGAEIGAGPIARLWRQSLVLASRNWQLESLYRSNAKYLPDWEPRFLCPDRFALAMFAFATLVVLVTVIPPWHRYFIRSSDVVSSLTIPIVPGFTYAALLIAMAVALRRRLRAAWWLLLIWWLVLPEVGRLVALASDPRITTAVGFVFIAGVIVVAIRVRDQFSSRQVAGSIAAAVACFVVGGLAVLGVGAVLVRRFGTADSLPAAALHVFDSMLGDLGRVSDGPVITAPLWVRVVTGLVGAAVVLTSAHLL"
  },
  {
    "reference": "MWSTRVKKLLLLSFNFLIISAIVSSCSIPEELQAKTIVNEFFPNFWVFVAHTIALSIIILLGIFLLWKPTKRFLAKRSELIQAEINNANEIKKQAQFLLDNAKKQKQNAELQAREIINLATNQAYRLKNDLETDAKRKANRIIENAHAEIIKQESILKRELEGRIVDVALEATSTLIQKNVAKEDHERLVNELLRNLD",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization.2. The designed protein localizes to the proton-transporting ATP synthase complex, coupling factor F(o), plasma membrane.3. For general function, the protein need meet that Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0). The designed protein sequence is ",
    "response#1": "LVDKI",
    "response#2": "MSSNTKP",
    "response#3": "LQPS"
  },
  {
    "reference": "MEESSVTIGTIDVSYLPNSSEYSLGRCKHTNEDWVDCGFKPTFFRSATLKWKESLMSRKRPFVGRCCYSCTPQSWERFFNPSIPSLGLRNVIYINETHTRHRGWLARRLSYILFVQERDVHKGMFATSITDNVLNSSRVQEAIAEVAAELNPDGSAQQQSKAIQKVKRKARKILQEMVATVSPGMIRLTGWVLLKLFNSFFWNIQIHKGQLEMVKAATETNLPLLFLPVHRSHIDYLLLTFILFCHNIKAPYIASGNNLNIPIFSTLIHKLGGFFIRRRLDETPDGRKDILYRALLHGHIVELLRQQQFLEIFLEGTRSRSGKTSCARAGLLSVVVDTLSSNTIPDILVIPVGISYDRIIEGHYNGEQLGKPKKNESLWSVARGVIRMLRKNYGYVRVDFAQPFSLKEYLEGQSQKPVSAPLSLEQALLPAILPSRPDAAAAEHEDMSSNESRNAADEAFRRRLIANLAEHILFTASKSCAIMSTHIVACLLLYRHRQGIHLSTLVEDFFVMKEEVLARDFDLGFSGNSEDVVMHAIQLLGNCVTITHTSRKDEFFITPSTTVPSVFELNFYSNGVLHVFIMEAIIACSIYAVQNKRGSGGSAGGLGNLISQEQLVRKAASLCYLLSNEGTISLPCQTFYQVCQETVGKFIQYGILTVAEQDDQEDVSPGLAEQQWNKKLPEPLNWRSDEEDEDSDFGEEQRDCYLKVSQAKEHQQFITFLQRLLGPLLEAYSSAAIFVHTFRGPVPESEYLQKLHRYLLTRTERNVA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein can be expressed in plasma membrane and perform sn-1-glycerol-3-phosphate C16:0-DCA-CoA acyl transferase activity without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "YAKELRGATKEKESLPQMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNIAADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDSTVPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMAAIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQDDELHINPSHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLSVLHGINAPEFFDKAVFSSLVLTLRDEGYISC",
    "response#2": "YQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLRNLGQGYVNFGEPMPLMTYLV",
    "response#3": "INVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLDPKTGTLSMTIQAMLRGGTRPITLIPIYY"
  },
  {
    "reference": "MSLEKDKSTVINNYKLHESDTGSADVQIALLTDRINQLIEHLKLHKQDHHSRRGLLKLVGRRRRLLAYLSKKDNARFRAVSERLGLRIKA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The ideal protein would be one that plays a critical role in translation through rRNA binding within cytoplasm, ribosome, ribonucleoprotein complex.2. For general function, the protein need meet that Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome. The designed protein sequence is ",
    "response#1": "GQPL",
    "response#2": "LAPGFR",
    "response#3": "LFLGH"
  },
  {
    "reference": "MAVANDIIRTDIEQYLNQHERKGLLRFITCGSVDDGKSTLIGRLLCDSKALFADQLETVRAESKKFGTQGSDLDLALIVDGLAAEREQGITIDVAYRYFNTERRKFIVADTPGHEQYTRNMITGASTADVAILLIDARKGILTQTRRHAYLASLIGIRNVVLAVNKMDLVKYSEKVFTDIVDRFNEFSSQLEYSHVTAIPMSALIGDNVIEPSVEMPWYHGTTLLSYLETVKIPTDEIQQSAFRLPVQWVNRPNLDFRGFAGTIASGSVKPGDAIRVQPTGKTSTVDRIVTYDGDLEEAVAGQAVTITLKDQIDISRGDIISTTETPASTANQFESTLVWMDEEPLLPGRSYYLKCGTATATATITDIKHEINVNTLEQTAAKKLELNNIGSCNLNLDRQLAFDTYQENQSTGSYILIDRATNHTVGAGTLNFALRRSQNIHMQAVDVDKGLRSELKEQKACVLWFTGLSGSGKSTIANIVEKKLAAKGRHTYLLDGDNVRHGLNKDLGFTDADRVENIRRIGEVARLMVDSGLIVLTAFISPFIAERQMARSLLEDGEFLEIYVETPLAIAEERDPKGLYKKARRGELKNFTGIDSPYEHPESPEILVDTSSMSAEQCAERVIAILRQRGVIQEG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have sulfate adenylyltransferase (ATP) activity to facilitate sulfate assimilation. The designed protein sequence is ",
    "response#1": "PPHGGILKDLIARDAPRHDKLEAEAETLPAITLNERQLCDLELILNGGFSPLEGFMNEKDYNGVVENVRLADGALFSMPICLDASAETIKAKKIQAGSRITLQDFRDDRNLAILMVDDVYQPDKEKEAKLVFGGDPEHPAIRYLFNQTEKYYIGGKLEAVNK",
    "response#2": "ELLRFITCGSVDDGKSTLIGRLLFDSKMLFEDQLATIERDSKKWGTQAEAIDLALLVDGLAAEREQGITIDVAYRYFATDARKFIVADTPGHEQYTRNMVTGASTADAAILLIDARRGVLTQTRRHGYLAHLLGIRHLVLAINKMDLMEYAESVYDQIVADFQAFAGMIGISAFTAIPVSALTGDNVTEPSAHMGWYTGPSLLKLLED",
    "response#3": "PPHGGILKDLIARDAPRHDKLEAEAETLPAITLNERQLCDLELILNGGFSPLEGFMNEKDYNGVVENVRLADGALFSMPICLDASAETIKAKKIQAGSRITLQDFRDDRNLAILMVDDVYQPDKEKEAKLVFGGDPEHPAIRYLFNQTEKYYIGGKLEAVNR"
  },
  {
    "reference": "MSAKAVSELSGKEVLYKYFEPSGLLSAPHAFHVKAGENFDEIANKYEWLARDNKGVIKPDQLIKRRGKLGLVKIGTPQELKAWFEKTGDSYVRVGQTEGRLHTFIVEPFCAHTEKDEMYIAIYSERFRDVIMFYEQGGVDIGDVEEKARTVSVPVQLNENAMTPSDEELTTLLGPLKDSDIVRRFVVELYKAYKDLHFTYLEINPFVLLNNQIHVLDLAARLDETANFLCADKWKSRLTPYGGPNHVEFPAPFGRDLTSEEQYISEMDAKTGASLKLTILNRKGRVWTMVAGGGASVVFTDTVCDLGGASELANYGEYSGDPSESQTYEYAKTLLSVMTEGTPRPDGKVLIIGGSIANFTNVAKTFGGIVRAFETFVSKLKEHKVTIFVRRGGPNYQEGLRRIKDAATKLELPIHVFGPETHMTAIVGAALGVKPMPTVPTAPQTTGQFLLSPERNTGGTERAPPSPAANATPTEHPLTTAQQNKLKSFRGLFEDDTKAIIWGQQAKAIQGMLDFDYVCRRSSPSVVASTYPFTGDNKQKYYFGQKEILIPAYKSMAKAFATHPDASIMVTFASMRSVFETVLEALEFPQIKVIAIIAEGVPENQTRKLLKIAHDRGVTLVGPATVGGIKPGCFKIGNTGGMMDNILASKLYRPGSVAYVSRSGGMSNELNNIISQNTNGVYEGIAIGGDRYPGSTYTDHVIRYQNDDRVKMIVLLGEVGGVEEYKIVDLLKQKKVTKPLVAWCIGTCADHITSEVQFGHAGASANAL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be able to bind ATP, CoA, Mg(2+), citrate ligand in a variety of conditions2. For general function, the protein need meet that ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues.3. The protein with the reaction acetyl-CoA + ADP + oxaloacetate + phosphate = ATP + citrate +   CoA, the Tele-phosphohistidine intermediate must be optimized to ensure efficient catalysis.4. A protein that contributes to citrate metabolic process, tricarboxylic acid cycle, fatty acid biosynthetic process by functioning in cytosol with ATP citrate synthase activity, ATP binding, metal ion binding. The designed protein sequence is ",
    "response#1": "MSEKILYSHLDDPHGQEIERGKSYLKLRPDRVACQDATAQMAILQFMSAGMPSVATPATVHCDHLIEAQLGGDKDLARANEINKEVYDFLSTSCAKYNIGFWKPGSGIIHQILLENYCFPGGLMIGTDSHTPNGGGLGMAAIGVGGADAVDVMAGLPWELKAPNVIGVKLTGQMSGWTAPKDIILKVAGILTVKGGTGAIIEYHGDGVNSLSCTGMGTICNMGAEIGATTSVFPFNDRMYDYLKATKRQSIGDFSRVYAEGLRPDENAQYDQLIEINLSELEPHINGPFTPDLATPISKFKEAVKENNWPSELKVGLIGSCTNSSYEDMSRAASIARDALNHGIKAKSLFTVTPGSEQIRATIERDGQLKTLEEFGGVILANACGPCIGQWDRKDVKKNEANSIISSYNRNFTGRNDANPATHAFVTSPDLVVALTIAGRIASPEEILGQ",
    "response#2": "MEKILYSHLDDPHGQEIERGKSYLKLRPDRVACQDATAQMAILQFMSAGMPSVATPATVHCDHLIEAQLGGDKDLARANEINKEVYDFLSTSCAKYNIGFWKPGSGIIHQILLENYCFPGGLMIGTDSHTPNGGGLGMAAIGVGGADAVDVMAGLPWELKAPNVIGVKLTGQMSGWTAPKDIILKVAGILTVKGGTGAIIEYHGDGVNSLSCTGMGTICNMGAEIGATTSVFPFNDRMYDYLKATKRQSIGDFSRVYAEGLRPDENAQYDQLIEINLSELEPHINGPFTPDLATPISKFKEAVKENNWPSELKVGLIGSCTNSSYEDMSRAASIARDALNHGIKAKSLFTVTPGSEQIRATIERDGQLKTLEEFGGVILANACGPCIGQWDRKDVKKNEANSIISSYNRNFTGRNDANPATHAFVTSPDLVVALTIAGKIKTVEEL",
    "response#3": "EKILYSHLDDPHGQEIERGKSYLKLRPDRVACQDATAQMAILQFMSAGMPSVATPATVHCDHLIEAQLGGDKDLARANEINKEVYDFLSTSCAKYNIGFWKPGSGIIHQILLENYCFPGGLMIGTDSHTPNGGGLGMAAIGVGGADAVDVMAGLPWELKAPNVIGVKLTGQMSGWTAPKDIILKVAGILTVKGGTGAIIEYHGDGVNSLSCTGMGTICNMGAEIGATTSVFPFNDRMYDYLKATKRQSIGDFSRVYAEGLRPDENAQYDQLIEINLSELEPHINGPFTPDLATPISKFKEAVKENNWPSELKVGLIGSCTNSSYEDMSRAASIARDALNHGIKAKSLFTVTPGSEQIRATIERDGQLKTLEEFGGVILANACGPCIGQWDRKDVKKNEANSIISSYNRNFTGRNDANPATHAFVTSPDLVVALTIAGQICF"
  },
  {
    "reference": "MRLFENSKDMENRKRLLRAKKAAGNNNCFECKSVNPQFVSCSFGIFICVNCANLLRGMGTNIFCVKSITMDNFEEKDVRRVEKSGNNRFGSFLSKNGILQNGIPLREKYDNLFAKSYKRRLANEVRSNDINRNMYLGFNNFQQYTNGATSQIRDRTLREISNNSNASEGAEFVLPEKVLGSDNFQDCERFPACLSSERNLDENNVTSATSTLTIEKFQNDPIGTISRSWQLLSDALYKSYEDFKGSVVQPTIENIQQRNLPNDIKRSFVHFNEKLHETPHLPSPVFSCFTGGDILPPEFN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must have at least one Arf-GAP domain.2. The protein should be optimized for stability and expression in Golgi membrane where it will participate in regulation of ARF protein signal transduction, ascospore formation. The designed protein sequence is ",
    "response#1": "EESRMAQKEKELCIGRKKFNMDPAKGIQYFIEHKLLTPDVQDIARFLYKGEGLNKTAIGTYLGERDPINLQVLQAFVDCHEFANLNLVQALRQFLWSFRLPGEAQKIDRMMEAFATRYCLCNPGVFQSTDTCYVLSFSIIMLNTSLHNPNVRDRPPFERFVSMNRGINNGSDLPEDQLRNLFDSIKSC",
    "response#2": "GIPKQIRGIIWQLISNSKSKEIRQLYQDLLQIPSEHEKAIQRDISRTKFIPVDKTESLFNVLKAYSLFDPEVGYTQGMAFVTAPLLINVWEEADAFGLLIKLMKNYGLREFFLPDMPGLQLKLYEFDRLLEENSPQLYNHLIRLGIRSSMYATQWFLTLFAYKFPLGFVLRILDVIFVES",
    "response#3": "GIPKQIRGIIWQLISNSKSKEIRQLYQDLLQIPSEHEKAIQRDISRTKFIPVDKTESLFNVLKAYSLFDPEVGYTQGMAFVTAPLLINVWEEADAFGLLIKLMKNYGLREFFLPDMPGLQLKLYEFDRLLEENSPQLYNHLIRLGIRSSMYATQWFLTLFAYKFPLGFVLRILDVIFVER"
  },
  {
    "reference": "MGNLRRRRAGGRGWRWCRRRGRGGPERVEGGPASFPRVPGSCAWPGELGRAACLLPPEARWPFAAAAGRSPPSAPAGPGLRGAGVQGSACSQPLRLAWGPHRARGRVGPAPEAGRGPRPALKPRKCGSRSGREARERRGRVRRASRPCEALAPEQADPAGPERPGRPGDLLRRPFLLPRRGWRWRRGGGGGAGAGGGAGGGGGSRLRARGAALRGGPRAAGSRLPPRAGLPATPLEPTIFPRERRGRLSCAPPLRLAASLLNLPFRSRRPPRWLPPRALRRPLCLLFTGCLFPEARKKSFLFSALYAAFIFGGRHLMNKRAKFELRKPLVLWSLTLAVFSIFGALRTGAYMVYILMTKGLKQSVCDQSFYNGPVSKFWAYAFVLSKAPELGDTIFIILRKQKLIFLHWYHHITVLLYSWYSYKDMVAGGGWFMTMNYGVHAVMYSYYALRAAGFRVSRKFAMFITLSQITQMLMGCVINYLVFYWMQHDQCHSHFQNIFWSSLMYLSYLVLFCHFFFEAYIGKRRKTTKAE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: May participate to the production of saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.; Catalyzes the synthesis of unsaturated C16 long chain fatty acids and, to a lesser extent, C18:0 and those with low desaturation degree; Condensing enzyme that elongates fatty acids with 12, 14 and 16 carbons with higher activity toward C16:0 acyl-CoAs; This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle.2. The protein should be designed to catalyze the following reaction dodecanoyl-CoA + H(+) + malonyl-CoA = 3-oxotetradecanoyl-CoA +   CO2 + CoA.3. The protein should exhibit Basic and acidic residues compositional bias.4. The designed protein should have fatty acid elongase activity within endoplasmic reticulum membrane to regulate long-chain fatty-acyl-CoA biosynthetic process, unsaturated fatty acid biosynthetic process, very long-chain fatty acid biosynthetic process, fatty acid elongation, monounsaturated fatty acid. The designed protein sequence is ",
    "response#1": "MEQILKNNIPLSQLSQHTPRLIDKVQSRFYKDFNIKYINGEVVFNGKDLNNKISIIRGGGYDWRIEYTPYPERQTRIKIVNSNFLYNLKENEEYLNREINKLKNKISHIIENNTIVLDIGGCTGKYAYEFIRNAIYVDAIDPLSEQLNICKEYFKNSNFINAKILGEGEKIQLNSNFYDTILLIFLHWYHSYNFEEVLSEAHRVLKKGGILYITNLYKNTDQFFSYFLKIKEGDERKYFNNEGNILEFKNKFETIDLINKLEFEIVDFKGLKSKRNNLNLLQDYFVLIAQKIS",
    "response#2": "MDFQSLLALAGRTIPGGVNSPVRAFTGVGGTPLFIERADGPYFFDAEGNRYIDYVGSWGPMIVGHNHPKVIEAVQKAAQRGLSFGAPTEMEVKMAELVTELVPTMDMVRMVSSGTEATMSAIRLARGFTGRNKIVKFEGCYHGHADSLLVKAGSGALTLGQPSSPGVPEDFAKHTLTLTYNDLASVRAAFEQYPQDIAGVIVEPVAGNMNCVPPKPGFLEGLREICDQEGAL",
    "response#3": "MEPNVNGTALGEHSVGLVDNHGNAGNDSSGPSSSDPPEPGPPSRVPPPLSPERELYRKFALPLGKTLLYITFLHVYHSCFHPSALQVFYNSLGKYSWGAAMAFCLGVFCFIFFSGYQWLILWKDGVRRTSYFLPMSISAVILFVNTCRWFQAHHSLSPYPYDTAIVLAREGLILAAVSALGVFLSYFSSLKAGPIKSHPNDLMDFRHPNGATLTLKWARRKGFSILYLITLGLCHLQATLLPSLALTGFHLFPHGWLFHPLNTTIYTLHGLILLFLFGLQTFVDVPSLGKGFKWYRLFLAGGMWTLLTVPVELYVTLLGVFYPSVPYTLLLPFHGFILLLSLGIFFQCYSEVTLSRAPMEDHPNGCTHGHEDGLEAGAGGSAGRGGMGNCISNCISNCISNCISNCISNCISNCISNCISNCISNCISNCISNCISNCMSNCISNCISNCISNCISNCISNCISNCISKCISNCISNCISNCISNCISNCISNCISNCISNCISNCISNCVSNCISNCISNCISNCISNCISNCISSCISNCISKCISNCISNYISSCISNCISNCISNCISNCIS"
  },
  {
    "reference": "MTLLGSEHSLLIRSKFRSVLQLRLQQRRTQEQLANQDIMLSLQNQTIFQEQRKNFDKTEDYLKHKIRKRPEGTNLINMHILQDTTAEESITAPQMKLKRARLADDLTENIALRPGLLELVEKNRIPVDSTMKESMKGNHVGISKSTDVFAFDEDSSNDELSPEQTRSEDSQGSNSFPPDTKATQASSSAPSETTQDHTSGSENDRNEPVSHFINQSDNMKQSKSFSDSKNRHKKPKDTKPKVKKLKYHQYIPPDQKAEKSPPPMDSAYARLLQQQQLFLQLQILSQQQQQQRFSYPGHHQAQLKEPNEELIKNPNSSVSISNNPLSPAKPTFSGQTGISSLKLGPLPSNLDDLKVSELRQQLRIRGLPVSGTKTALMNRLRPFQDCPENTGPNFSDITTITFPVNPANTLTSYQSSSTSVLPSGFYHFGSTSSTPPISPASSDVSVNGSLPDTFNDTSPSFVLQPSPIHICAEESLMSSLNGGCIQSDLDGIDSEKDKMLVEKQKVINELTMKLQQEQRQVEELRMELQKQKRNNYTEKKQPQTNFFGIHIKKEDTVCSCPFPSKQTSVKIQTNNSEKQLSTREPAQLLPPLNSHSVESIGQTKVLSSTFLSPQNSPQHSPVGAVKSPQCISRPPSPNNHYHLSSSSSSQGEKHSGLSQNSSQMCSIQSSGIQDGSTSGFSPESSKLRIPFLGTQPDSRQVTRVNASSKSPDTKHKQMIQSQQMDELLDVLIESGEMPANVKEDHSCFQKAQKITGSSQSLLHKSSSP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have transcription coactivator activity, histone deacetylase binding, RNA polymerase II-specific DNA-binding transcription factor binding, histone acetyltransferase binding within nucleus to regulate negative regulation of transcription by RNA polymerase II, lung alveolus development, positive regulation of miRNA transcription, negative regulation of cell adhesion molecule production, negative regulation of platelet-derived growth factor receptor-beta signaling pathway.2. Include a signal peptide as an essential component of the protein.3. The protein should exhibit Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "AKKTPEEMALNYECAMFGDSQAVLKGARHMAAVEISCEPSIRKYVRGIFMENAVVSTSPTPDGNVVIDSFHRFSAVKWLSEKPLRKFDGVQWLLIQKAEEEKLLQVTFKLPENQMNRIISECSEHYLSVGVSKYAQLWNEQRKLILEDAVHGFVLPSMEKEARSLLTSRAQ",
    "response#2": "GRKIIPPLTIGVPISFLEACGKYLLCVSSIGELYCWNIASSKLEFPVTTIFPLLSPSIRYSDDILTRC",
    "response#3": "AKKTPEEMALNYECAMFGDSQAVLKGARHMAAVEISCEPSIRKYVRGIFMENAVVSTSPTPDGNVVIDSFHRFSAVKWLSEKPLRKFDGVQWLLIQKAEEEKLLQVTFKLPENQMNRIISECSEHYLSVGVSKYAQLWNEQRKLILEDAVHGFVLPSMEKEARSLLTSRAY"
  },
  {
    "reference": "MPPKRNFRKRSFEEEEEDNDVNKAAISEEEEKRRLALEEVKFLQKLRERKLGIPALSSTAQSSIGKVKPVEKTETEGEKEELVLQDTFAQETAVLIEDPNMVKYIEQELAKKRGRNIDDAEEVENELKRVEDELYKIPDHLKVKKRSSEESSTQWTTGIAEVQLPIEYKLKNIEETEAAKKLLQERRLMGRPKSEFSIPSSYSADYFQRGKDYAEKLRREHPELYKDRGGPQADGEAAKPSTSSSTNNNADSGKSRQAATDQIMLERFRKRERNRVMRR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Inhibits E3 ubiquitin-protein ligase activity of COP1, a central repressor of seedling photomorphogenesis2. The designed protein should have a specific function within the spliceosomal complex. The designed protein sequence is ",
    "response#1": "GMRNHPLDLPVQFTASACALNLTRQELARGMPVRLLAEITDLLFKATKNFPYYQQLQKNCLLSLTSSRILMDVPFDRFDAAKLALRWVCRRESPKLRTMAVSITSILALKLSPEEMGQLQEELIMAIKELLTIIRQKLAENLDDVTFLFTLKALWNLTDECPLACKYFMENEGLATVIRVLETFSISVIQSKVLGLLNNVAEVRELSSKLVTEDVIERIISLLHSSNLEVSFLAAGVLAHLTCDRQHWLSRDLQRTDLLRYLHLAIQNWPSSRCDMSVLVTYRSFKAFSPLLVNFSQPEVQRWALWAIHHVCSKNPL",
    "response#2": "QFLEAAKCYYRVLQYKVPGNELIYRENLCSVAQCLLLAIPSPIVLQFLQEISLQPSVREIPFYSLVEKYLKRKFIGKEDGAFLLPFLLPHQVIHMNRLIEDGRHFLETNILFLSEFFEVSSTSILAKHFKLSEEQVDTVVADMVIQERLNASIDQCEGYITFLPEYH",
    "response#3": "GMRNHPLDLPVQFTASACALNLTRQELARGMPVRLLAEITDLLFKATKNFPYYQQLQKNCLLSLTSSRILMDVPFDRFDAAKLALRWVCRRESPKLRTMAVSITSILALKLSPEEMGQLQEELIMAIKELLTIIRQKLAENLDDVTFLFTLKALWNLTDECPLACKYFMENEGLATVIRVLETFSISVIQSKVLGLLNNVAEVRELSSKLVTEDVIERIISLLHSSNLEVSFLAAGVLAHLTCDRQHWLSRDLQRTDLLRYLHLAIQNWPSSRCDMSVLVTYRSFKAFSPLLVNFSQPEVQRWALWAIHHVCSKNPL"
  },
  {
    "reference": "MVLIRVLANLLILQLSYAQKSSELVIGGDECNINEHRSLVAIFNSTKFFCSGTLINQEWVVTAAHCDSTNFKMKLGVHSKKVPNENEQTRNPKEKFFCPNKKKDDVLDKDIMLIKLDSPVSNSEHIAPLSLPSSPPSVGSVCHIMGWGSITPIEKTLPDVPYCANINLLDDAVCRPPYPELPATSRTLCAGILEGGKDTCRGDSGGPLICNGQFQGIVFYGAHPCGQALKPGVYTKVFDYNDWIQSIIAGNTAATCPP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Snake venom serine protease that may act in the hemostasis system of the prey.2. The protein design should be able to enhance the efficiency of proteolysis.3. The protein contains novel Peptidase S1 domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "IMGGSECYKSKHPFLVYLYNSAGFFCSGTLLNHEWVLTAAHCNRDDIQLKLGVHNVHVHYEDEQIRVPKEKLCCLSTKNCTQWSQDIMLIRLNSSVNNSKHIEPLSLPSRPPSMGSDCTVMGWGTITSPKVTYPKVPHCVDIKILHNPVCQAAYPTMSRKNILCAGVLEGGKDSCKGDSGGPLICDGQIQGIVSWGRFPCAQLLEPGVYTKVFDYIDWIRGIIN",
    "response#2": "IMGGSECYKSKHPFLVYLYNSAGFFCSGTLLNHEWVLTAAHCNRDDIQLKLGVHNVHVHYEDEQIRVPKEKLCCLSTKNCTQWSQDIMLIRLNSSVNNSKHIEPLSLPSRPPSMGSDCTVMGWGTITSPKVTYPKVPHCVDIKILHNPVCQAAYPTMSRKNILCAGVLEGGKDSCKGDSGGPLICDGQIQGIVSWGRFPCAQLLEPGVYTKVFDYIDWIRGIIR",
    "response#3": "IIGGDECNINEHRFLVALYTSRSRRFHCSGTLINQEWVLTAANCDRKNIRIKLGMHSKNVTNEDEQTRVPKEKFFCLSSKTYTKWDKDIMLIRLKRPVNDSPHIAPISLPSSPPSVGSVCRIMGWGTISPTKVSYPDVPHCANINLLDYEVCRAAHGGLPATSRTLCAGILEGGKDSCQGDSGGPLICNGQFQGILSWGVHPCGQRLKPGVYTKVSDYTEWIRSIIS"
  },
  {
    "reference": "MSFSAVILAAGKGTRMYSNLPKVLHTLAGKPMVKHVIGICNDLGANNIHLVYGQGGDQMKQVLSQEQVEWVLQAEQLGTGHAVNQATPNLADDEQVLILYGDVPLISQTTLENLLDAQPAGGIALLTVVLDDPTGYGRIVRRNGPVVAIVEQKDATEEQKLIKEINTGVMVVSGGDLKRWLSALKNDNAQGEYYLTDIIAAAHEEGRAVEAVHPEKAIEVEGVNNRIQLARLERAFQAMQAERLLEQGVMLRDPARFDLRGDLQCGTDVEIDVNVIIEGSVSLGNNVVIGAGCVLKDCEIDDNTEIRPYSVIEGATVGEDCSVGPFTRLRPGAELMRDAHVGNFVEMKQARLGEGSKAGHLTYLGDAEIGANVNIGAGTITCNYDGANKFKTEIADDVFVGSDTQLIAPVKVGKGATIGAGSTINKDVNDGELVITRAPMRNIQGWKRPVKK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The UDP-N-acetyl-alpha-D-glucosamine binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).3. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.4. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining glucosamine-1-phosphate N-acetyltransferase activity, magnesium ion binding. The designed protein sequence is ",
    "response#1": "VIVLAAGMGTRMRSDTHKVLHPIAGKPMLMHLLDTVDSLGAKERVVVVGKGRDQVEMALNGRGVKIAHQAEQKGTAHAVQQAQGVLPEFDGNILILYGDTPFVAAETLREMLQRLDGPDGPAIVVLTSSPGDPKAYGRIIVEDGNLLKMVEYKDANAEERAVNLCNSGVMAVDGKLLLDLLEKIDNENAKGEYYLTDIVEIARDRGHKASAVICDEAETLGVNTRVQLAEADAIFQSKARVALMEAGVTLHAPDTVYLSHDTHIGPDTIIEPNVVFGASVTVENGANIRAFSHLEGCHVSRGATVGPYARLRPGTELAEFTRVGNFVEIKNAVIDEGAKVNHLSYVGDAHVGARANIGAGTITCNYDGVMKHHTEIGEDSFIGSNTLLVAPVAIGANAMTASGTVVTQDVPDDALAIARSKQVNKDGRGKDLFERLRALKDAKK",
    "response#2": "IILAAGKGTRMKSDLPKVLHHIAGAPMLVHAMKSGATLDPAKTIIVAGHGAEQVSDAAKAFDETADVVLQTEQLGTAHAVAQAKEVLFDFDGDALVLYGDTPFINPKTLEHMTAARSDHDVVVLGFLTARPADPTGYGRVVCENGQVVQIVEDKDCTPAQREIAEVNSGIYCFDVKWLWPHLQQLKPSAQHGEYYLTDTVALAVQDGLTVAAHQTDDPIEVLGINDRVQLAEAEARLRQRINEHWMREGVRLADPARIDVRGRVLAGMDVEIDIDVVLEGAVELGDEVQVGPFCRIRNSTLAAGTVIQGHCELDGVVTEGPCTIGPFARLRPGTRLAAGVHIGNFVETKNATLGEGSKANHLSYLGDAVIGARVNIGAGTITCNYDGANKHRTTIEDGAFIGSNSALVAPVTVGAGATLGAGSTLTRDAPAGQLTVARARGVPARVIDGWERP",
    "response#3": "VILAAGKGTRMKSRLPKVLHEIAGRPMLGHVLANTRFERDTRTVLVVGPDMDRVSDYARSVRPDIAVVVQQKQLGTGDAARTALPELHDSDGVVLVVFGDTPLVRAETLENMADACRKSGSIVILGFEAADPTGYGRVVRDGEELVAIVEHKDASEASRSIREINSGVYAFDAAVLRRGLSRLTSDNAQQELYLTDVLAILRGDGHRVGAHRVDDAWLVEGVNDRVQLATLGAELNRRVVEAHMRAGTTIGEAAQIGPFARLRPGADIASDVKIGNFVEVRSDTHIGPDTIIEPNVVFGASVTVENGANIRAFSHLEEALEQGATLVDPESVWFSHDTQLGRDSTIEPHVVFGPGVVIEDFATIRAFCHIEGATIASGCEVGPFARLRPGTVLEQGAKVGNFVEVKKARLGEGAKANHLSYIGDADVGAKANIGAGTITCNYDGFGKHRTEIGAGAFIGSNTALVAPVSVGEGAIVGAGSVITRDVAADELAVARGQGVPAHAVRAIVLAAGEGTRMKSDRPKALHEVAGRSMLGNVLSSAMSAGVGSVAVIVGPGRDDVGAEARRFIPEAEIFVQSERRGTAHAVLAARHAIAKGADDLLVLFADTPLLTAPTIGALRAALAEGAAVAVLGFQASNPFGYGRLLRDAAGRLVQRRER"
  },
  {
    "reference": "MSNTGITLALSKGRIFDETLPLLAAAGITPAESPESSRKLIIGTNRDDVRLIIVRASDVPTYVQYGAADLGIAGKDVLNEHGGRGLYQPLDLQIAKCRMMVAVREGYDYAGTVKQGARIRVATKYVNAARDHFASKGVHIDLIKLYGSMELAPLVGLSDVIVDLVSTGGTLKANGLAAVEHICDISSRLVVNQAALKLKRSVIQPVVDAFAAAVAR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have ATP phosphoribosyltransferase activity, ATP binding to facilitate histidine biosynthetic process.2. The protein must exhibit the following characteristics: Catalyzes the condensation of ATP and 5-phosphoribose 1- diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP); Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity..3. The protein have enhanced 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-   alpha-D-ribose 1-diphosphate + ATP.4. A protein should be able to manipulate the activity of enzymes in the Amino-acid biosynthesis, L-histidine biosynthesis, L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9 pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "LTVAMPKGRIFDEAVGLLRKAGYNLPAEFEASRKLIVDVPEENMRFILAKPMDVPTYVEHGVADVGVAGKDVMLEEERDVYEVLDLKISECYLAVAGLPNYEKKHDLNPKVASKYPHLATRYFKEQGEQVEIIKLNGSIELAPLIGLADRIVDIVSTGRTLRENGLVELEKMMTITSRLIKPMDVVTYVEHGVADLGIAGKDVMMEEERDVYELLDLKISKCHLAVAGLPGVRMNQIAPRVATKYPNIASSYFREQGEQVEIIRLNGSIELAPLIGLADRIVDIVSTGRTLKENGLVELEKIADVTSRLIVNPVSYRMKDEAVDELVHRLAKESKYILLHAPTETLEQIVALLPGAENPTVLPLNDDTNRVAIHAVSTEDLFWDTMEQLTQLGASSILVMPIEY",
    "response#2": "MTDLTIAIPKGGLLPEAIALLQQVGLDFSAFLDKKNRQLQITDPTGTARAMLVRTHDVPVYVEYGQAQLGFAGYDVLREKKPDVANLLDLGFGGCRLSVAVPKASPYKNPRQLPPNCRVASKFVNCAKDYFRDLDLPIEVIPLHGSVELGPITGMSEAIVDLVSTGNTLRENNLEEVEVLFHSTVRLIKAPDVPTYVEHGAVDMGIVGKDVLLEANKQLYEVLDLQFGKCKFSVAGSTSMENYPTPHMRVATKYPNIAKNFFQSKGQSVEIIKQEGSVELAPMVGLADVIVDIVETGNTLRENGLMVFEDIVEISARLVLNQVSYKTKHQEIRGLIDRIKAKDVETYVEYGAADIGIVGKDTLMETNKEFYEVLDLNVGKCKFALAALPSFKLDQGYNRKKIATKYPNIAREYFRKKGMDVELIKIEGSVELGPIVGLADAIVDIVETGNTLRENGLVVVEDICEISARMIVNKASMKTKKDEIIKIIENE",
    "response#3": "MLTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGTNHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVAVRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDAIVDLVSTGNTLKANGLEAVEHIVDISSRLVAKESKYILLHAPTETLEQIVALLPGAENPTVLPLNDDTNRVAIHAVSTEDLFWDTMEQLTQLGASSILVMPIERLKPMDVVTYVEHGVADLGIAGKDVMMEEERDVYELLDLKISKCHLAVAGLPGVRMNQIAPRVATKYPNIASSYFREQGEQVEIIRLNGSIELAPLIGLADRIVDIVSTGRTLKENGLVELEKIADVTSRLIVNPVSYRMKDEAVDELVHRLKPMDVVTYVEHGVADLGIAGKDVLMEEERNVYELLDLQISRCHLAVAGLPGVKMNGIAPRVATKYPNVASTYFREQGEQVEIIRLNGSIELAPLIGLADRIVDIVSTGRTLKENGLVELERIADVTSRLIVNPASYRLNGEVIERLVDRLRATDVPVYVEYGQAQLGIAGYDVLLEKSPDVANLIDLKFGYCRMSVAVPADSPYQSPLDIPHHGKVASKFVNCAKDYFRRLDIPVEIIPLYGSVELGPITGMSEAIVDLVSTGRTLRENGLVEIDELFASSARLIAHPLSYRLDRDQIYNWVEKLYS"
  },
  {
    "reference": "MTTSAASWRSDPADPSVASARERALRQRSEGRVRITASAFGGVTRLTDLAEGGALRARLPRGGPGLEAVIVNTAGGVACGDVFSIEAKAGPGAHLTVATPAAEKVYRSDGLCADIQVRLVAEAGARLDWLPQETILFDRARLRRRYEIDLSATASFLSFEALMLGRLAHGDAMGEGHLEDHWRLRRDGALIFADALRLAGPMGALLARPAVAGGNRALATLLYVAPDAEARLEEARALLDAARCEAGASAWNGLLCVRLLAPDIETLRRDATSFLMAFRNAPLPRVWAT",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be optimized for nickel cation binding. The designed protein sequence is ",
    "response#1": "LRTSRTNPAKRPDGLDTCIATLKKNGADALIAIGGDDTLSVATKLFDKGVRVVGVPKTIDNDLAGTDFTFGFDTAVNVVTEAIDRVHTTAEAHNRVMVVEVMGRDAGWIAIYSGIAGGADVILIPEQPFDVDKVAAKIRRRHERGRYFSIVVVAEGAKFASKNGPVMQSGETDEFGHARLGGIANTLALELEKRTGFETRTVVLGHIQRGGSPTAFDRVLATRYGLGAIDAL",
    "response#2": "LRTSRTNPAKRPDGLDTCIATLKKNGADALIAIGGDDTLSVATKLFDKGVRVVGVPKTIDNDLAGTDFTFGFDTAVNVVTEAIDRVHTTAEAHNRVMVVEVMGRDAGWIAIYSGIAGGADVILIPEQPFDVDKVAAKIRRRHERGRYFSIVVVAEGAKFASKNGPVMQSGETDEFGHARLGGIANTLALELEKRTGFETRTVVLGHIQRGGSPTAFDRVLATRYGLGAIDE",
    "response#3": "LRTSRTNPAKRPDGLDTCIATLKKNGADALIAIGGDDTLSVATKLFDKGVRVVGVPKTIDNDLAGTDFTFGFDTAVNVVTEAIDRVHTTAEAHNRVMVVEVMGRDAGWIAIYSGIAGGADVILIPEQPFDVDKVAAKIRRRHERGRYFSIVVVAEGAKFASKNGPVMQSGETDEFGHARLGGIANTLALELEKRTGFETRTVVLGHIQRGGSPTAFDRVLATRYGLGAIDAL"
  },
  {
    "reference": "MKVKEVIFAGRSNVGKSTLFSALFKFEVRKGKKPGTTIRPNSFQVGSVIFTDLPGFGYVSGYSRNFSERVKDFVVEYIETNARRIVASVEVIDASSFLEIAERWEKRGYIPVEIEMFEFLNDVTPRVFLAANKMDKVDDITNLNKIAEMLGMQPPWEKWRHVIYPVCAKKGEVSALKRDLKQYLLSLNLRDAAKAFR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be able to bind Mg(2+), GTP ligand in a variety of conditions2. The protein design must be able to perform GTP binding, metal ion binding in order to contribute to division septum assembly.3. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.4. The protein contains novel EngB-type G domains that confer a unique function or activity.5. For general function, the protein need meet that Necessary for normal cell division and for the maintenance of normal septation. The designed protein sequence is ",
    "response#1": "EVAFAGRSNAGKSSALNALTRQNALARVSKTPGRTQQLVFFQVTPEAHLVDLPGYGYAKVPLDLQAHWQAFIDKYFRTREALKGLVVVMDIRHPLKDYDRQMLSYAVQRGLPAHALLTKADKLSRSQQMQSLQKVRKELQSAYGDSVSVQVFSGEDRTGVDEARGVIGGWL",
    "response#2": "EIVFAGRSNVGKSTLLNSLTGVKGLAKTSATPGKTRLINYFLINRDVYFVDLPGYGYAAVGHAEKALWGNLLSSYIIQRRSISLVVLLLDSRHPAMQSDRAMISFLESHDRPYGIVLTP",
    "response#3": "ELPEIALAGRSNVGKSSFINTMLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYLTTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESAIKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKA"
  },
  {
    "reference": "MTTSPEAQSAPQPGEAETVSFGYEEIPAEEKTARVGAVFSNVAKKYDIMNDAMSGGMHRLWKDRFVSRVKPRAGERILDMAGGTGDIAFRLARSGADITVCDINQDMLDVGMERAEKRGITGLEWSCQNAEQISFPDRGFDAYTIAFGIRNVTHIDRALAEAYRVLKFGGRFFCLEFSTTEWPGFSEVYDLYSHRVVPQIGKMLANDADSYRYLIESIRRFPTMPAFEAMIRDAGFRHTKVEPILGGLVAIHSGWKV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Ideal protein should have a binding affinity for key enzymes in the Quinol/quinone metabolism, menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2 pathway to enhance the overall efficiency of the pathway.2. A protein with 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity, S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity is needed to enhance ubiquinone biosynthetic process, methylation in a specific context. The designed protein sequence is ",
    "response#1": "MVEHFLIMFL",
    "response#2": "MSKLLVATHNPGKLREFAALLPDGLELVTARAFGLGGPEETEPTFLGNARIKAHAAAAASGLIALADDSGLSVDVLGGAPGIFSARWAGPAADFGRAMDLVAAIEGVPEAERGARFVCALALALPGGGERVVEGRVEGRILRAPRGAGGFGYDPLFYAPELGRTAAELAPEEKNRISHRGRALRALVPLLRGL",
    "response#3": "MSIILGIDPGSRITGYGVIRQQGRQLVYLGSGCIRTAAEELPLKLRM"
  },
  {
    "reference": "MIVSWSLKDAVVVVVGSGKNAYRRVNLCLSENSSKIFWFCRSSADGGFDVGHLQLDAKKSDAIEILPISEFDPVRSLTTLGKEETDFLVDAVFVSESNHHEKEILHRTCKRYRIPLNIIDNPSLCSFTLPATWSEPPLQISLSTSSNGCRLAQRLLRHVVSSLPSGMPEAIERFGRVRAITKTPEKKQWINHVSDFWPLEKLVRMTEDDLLAIVSDNFSLPLSSSQSSSLANSYESLSTTLDKPSLTLDPEAFPTHKRGSIALIGSGPGSPDLLTVAARKAIMKADYVLADKLVPEAVLQLIPRHTPLFIARKFPGNADKAQDELHQVAFDALSRGDYVVRLKQGDPYIYGRGGEEYLFFTQHGYVPTVIPGISSALMAPISAGIPVTHRGVADQFLVCTGTGQKGSMPKIPSFVPTQTTVFLMALHRLEILVQALIESGWPRVLPVCIAERVSCPDQRFIFSTLEDVVEEYNKYESLPPGLLITGYSCNTLRNTA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must possess uroporphyrin-III C-methyltransferase activity to support methylation, methionine biosynthetic process under certain conditions.2. We need a protein that can perform 2 S-adenosyl-L-methionine + uroporphyrinogen III = H(+) +   precorrin-2 + 2 S-adenosyl-L-homocysteine with high efficiency.3. The protein must exhibit the following characteristics: Siroheme synthase involved in methionine biosynthesis.. The designed protein sequence is ",
    "response#1": "VGGGPGDPDLLTFRALRLMQQADVVLYDRLVAPAIVALVRKDAERIYVGKARDKHALPQKEINQLLVDLAKQGKRVLRLKGGDPFIFGRGGEEIELLSENNIPFQIVPGITAASGCASYAGIPLTHRDYAQSCLFITGHLKDGSTNLNWTALVQPQQTLAVYMGTHGLEILSRELIKHGMAETTPAAIVQQGTTSEQRVYITTLKALPTIPKQHNVKPPSMIIIGDVL",
    "response#2": "VGAGPGDPELLTLKALQLMQQADVVVYDYLVSDEIMELVRRDADLICVGKRLGDHSVAQQDTNQMLVDLAKQGKKVCRIKGGDPFIYGRGGEEVQVLAANKVNYQIVPGITAAAGCSAYAGIPLTHRDHAQAIQFVTGHCKKDGQELDWQSLAKPNQTLAIYMGVIKSPHIQAELLKHGRNANTPVAIIENGTRKNQRVITGKLGELADLITRNSVVSPALLIIGEVS",
    "response#3": "VGAGPGDAGLLTLKGLQQIQQADIVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHCVPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCQAGIPFSVVPGITAASGCSAYAGIPLTHRDYAQSVRLVTGHLKTGGELDWQNLAAEKQTLVFYMGLNQAAAIQQKLLEHGMPSDMPVAIVENGTAIKQRVVDGVLTGLAALATQMESPALIIVGRVVGAGPGDAGLLTLKGLQQIQQADIVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHCVPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCQAGIPFSVVPGITAASGCSAYAGIPLTHRDYAQSVRLVTGHLKTGGELDWQNLAAEKQTLVFYMGLNQAAAIQQKLLEHGMPSDMPVAIVENGTAIKQRVVDGVLTGLAALATQMESPALIIVGRVGEVYLVGAGPGDPDLLTFRALRLMQQADVVLYDRLVTEEILRLTRRDVEQVYVGKKRDYHAVRQAEINQLMVRLAREGKRVLRLKGGDPFIFGRGGEEIDTLSAEGIPFQVVPGITAASGCAAYAGIPLTHRDHAQSCVFVAAHLKDGSMDLNWELLVQPQQTVVLYMALQGLEVLCRQLIAHGMSTDTPAALVERGTTPRQRVFTGTLKTLPNIVGAADVHAPTLVIVGDVVRLHPQARWYG"
  },
  {
    "reference": "PSFFLSPWMPSASLLVNLLSALFIPFVFGETEIRFAGQTEFVVNETSTTVIRLVVERIGDPANVTAIVSLYGDDTGDFFDTYAATFIPFGETSRTVYIAVCDDDFPEPDETFIFHLTLQKPSANVKLGWPRTVTVTILSNDNAFGIISFNMPFSITVSEPRGRNESVPLTLIREKGTYGMVTVTFEVEGGPNPPEEDLSPVKGNITFPAGKATVVYNLTVLDDEVPENDEIFLIQLKSVEGGAEINTSRSSVEIIIKKNDSPVRFIQNVYLVPEEDHILTIPVVRGKDDSGNLIGSDDCEVSISYVIITGNSTAHAQQNLDFIDLQPNTTIVFPPFIYESHLKFQIVDDAIPEIAESFQVVLLKDTLRGDAVLFGPSVVQVTIKPNDKPYGVLSFNSVLFERPVIIDEDTTTRFEEITVVRNGGTHGNVSVNWVLTRNSSDPSPVTTDIRPRSGVLHFAQGQMLASIPLIVVNDDLPEEAEAYLLQILPHTIRGGAEVSEPAELLFYIQDSDDVYGLITFFPVENQKIESSPGERCLSLSLTRLGGTKGDVKIFYSALYIPAGAVDPLRAKDGILNISKRSSLIFPEQKTQVTIKLPIRNDAFLQNGAHFLVQLEAVELVNIVPPIPPISPRFGEIQNISLVVTPDIANGEIGFISNLPIILHEPEDFAAEVVCIPLHRDGTDGQATVYWSLKPSGFNSKAVTLDDIGPFNGSVVFLSGQSDTTINITVKADDIPEMNETVTLSVDRVSVENQVLKSGYTSRDLIILE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The presence of G-protein coupled receptors family 2 profile domains is critical for the interaction of the designed protein with its intended targets.3. The ideal protein would be one that plays a critical role in negative regulation of adenylate cyclase activity through calcium:sodium antiporter activity, G protein-coupled receptor activity, calcium ion binding within cell surface, sarcolemma, postsynapse. The designed protein sequence is ",
    "response#1": "GNVMVMISFKIDKQLQTISNYFLFSLAIADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYRAKRTTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFYFPVTIMCFLYWRIWRETKKRQKDLPNLQAGKKDSSKRSNSSDENTVVNHASGGLLAFAQVGGNDHDTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIKKSYTCFGSIKEWCIAWWHSGREDSDDFAYEQEEPSDLGYATPVTIETPLQSSVSRCTSMNVMRDNYSMGGSVSGVRPPSILLSDVSPTPLPRPPLASISQLQEMSAVTASTTANVNTSGNGNGAINNNNNASHNGNGAVNGNGAGNGSGIGLGTTGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGASSNAANGGGGGPGAGAAASASLSMQGDCAPSIKMIHEDGPTTTAAAAPLASAAATRRPLPSRDSEFSLPLGRRMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKRQESKAAKTLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTIY",
    "response#2": "LSTLQLMYTVGYSLSLISLFLALTLFLFLRKLHCTRNYIHMNLFASFILRALVVLVKDMVFYNSYSRRPDSESGWMSYLSEISASCRSVQVLLHYFVGTNHLWLLVEGLYLHALLEPTVLPERRLWPKYLVVGWAFPMLFVIPWIFVRASLENTGCWAVNENKKIWWIIRGPILLCVTVNFFIFLKILKLLISKFRAHQMCFRDYKYRLAKSTLLLILLMGVHEFLFTFFTDDQVQGFSRLIRLFIQLTLSSFHGFLVALQYGFASREVKAELRKTWGL",
    "response#3": "GNVMVMISFKIDKQLQTISNYFLFSLAIADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYRAKRTTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFYFPVTIMCFLYWRIWRETKKRQKDLPNLQAGKKDSSKRSNSSDENTVVNHASGGLLAFAQVGGNDHDTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIKKSYTCFGSIKEWCIAWWHSGREDSDDFAYEQEEPSDLGYATPVTIETPLQSSVSRCTSMNVMRDNYSMGGSVSGVRPPSILLSDVSPTPLPRPPLASISQLQEMSAVTASTTANVNTSGNGNGAINNNNNASHNGNGAVNGNGAGNGSGIGLGTTGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGASSNAANGGGGGPGAGAAASASLSMQGDCAPSIKMIHEDGPTTTAAAAPLASAAATRRPLPSRDSEFSLPLGRRMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKRQESKAAKTLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTISGNVMVMISFKIDKQLQTISNYFLFSLAIADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYRAKRTTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFYFPVTIMCFLYWRIWRETKKRQKDLPNLQAGKKDSSKRSNSSDENTVVNHASGGLLAFAQVGGNDHDTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIKKSYTCFGSIKEWCIAWWHSGREDSDDFAYEQEEPSDLGYATPVTIETPLQSSVSRCTSMNVMRDNYSMGGSVSGVRPPSILLSDVSPTPLPRPPLASISQLQEMSAVTASTTANVNTSGNGNGAINNNNNASHNGNGAVNGNGAGNGSGIGLGTTGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGASSNAANGGGGGPGAGAAASASLSMQGDCAPSIKMIHEDGPTTTAAAAPLASAAATRRPLPSRDSEFSLPLGRRMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKRQESKAAKTLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTI"
  },
  {
    "reference": "MKTFSLKLNDIKRNWFYVDATNKILGRFASAISIRLRGKHKIEYTPHLDTGDYIIVLNASKILVTGQKKINKVYYHHTGYIGGIKQLRFEEIMLKNPAKVIEIAVKGMLPKGSLGRSMFKKLKVFSNENHDHIAQCPQFLNI",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have structural constituent of ribosome within ribonucleoprotein complex to regulate translation.2. The protein must exhibit the following characteristics: This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself; It is important during the early stages of 50S assembly.. The designed protein sequence is ",
    "response#1": "DLQIIAGQKPVVTKAKKL",
    "response#2": "MLPIGCRVTLRGERMYDFLERLIFIALPRVRDFQGIPRRGFDGNGNYNLGIKEHTIFPEISFDKTDAVKGLNITIVTTADNDDMARTLLERVGLPV",
    "response#3": "MQIR"
  },
  {
    "reference": "MACMKKALPFILMVLLQIGYAGMDILTKDVLNKGMSIYVLSVYRHGVATVVMAPFAFYFDNPVIAQNLFNLGMKYTTATFAIALYNTLPAVTFILALIFRLESVKFQSIRSAAKVVGTVTTVGGIMVMTLVKGPALDLFWTKGPSAQNTVGTDIHSSIKGAVLVTIGCFSYACFMILQAITLKTYPAELSLATWICLIGTIEGVVVALVMEKGNPSVWAIGWDTKLLTITYSGIVCSALGYYIGGVVMKTRGPVFVTAFKPLCMIVVAIMSSIIFDEQMYLGRALGATVICVGLYLVIWGKAKDYEYPSTPQIDDDLAQATTSKQKEQRRTVIESV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. The protein can be expressed in plasma membrane and perform transmembrane transporter activity without triggering undesirable cellular responses.3. The designed protein must have at least one EamA domain. The designed protein sequence is ",
    "response#1": "RWFVLGVICLLSCTNAMLWISFAPVADVTASFFKCSLDVVNYLSLVYLIIAIPVGFGASWLIDTLGLKYAIVFSSWLNMIGSIIRCGAIVPYLNPSGSYTGIYYLFTGQSLCAIAQPLVLFVPAKLASVWFPEHQRATANMIASMSNPLGVLLANIISPSVVTKEEYIAHMLGIYTVPAIAACILATAGIRAKSPPTPPSASAFNSASEPFIAGIRQLLTNRAYVILMLCFGAGIGIFTAISSFLEQILCFRGYSNLFAGVCGALFIFFGFIGAFVCGLYVDRTKKFKEVVKTCFALTALTSIAFALVINFREQTVLVACVCSLLGLFGFAISPVGMELAVECSYPVGEGSSTGLAFISGQIQGIIYMILFQKLTRPFATSGPSPCGMNQTEIYDWSTSMLVMAALCSFGSCIFIIFFHTKYL",
    "response#2": "RWFVLGVICLLSCTNAMLWISFAPVADVTASFFKCSLDVVNYLSLVYLIIAIPVGFGASWLIDTLGLKYAIVFSSWLNMIGSIIRCGAIVPYLNPSGSYTGIYYLFTGQSLCAIAQPLVLFVPAKLASVWFPEHQRATANMIASMSNPLGVLLANIISPSVVTKEEYIAHMLGIYTVPAIAACILATAGIRAKSPPTPPSASAFNSASEPFIAGIRQLLTNRAYVILMLCFGAGIGIFTAISSFLEQILCFRGYSNLFAGVCGALFIFFGFIGAFVCGLYVDRTKKFKEVVKTCFALTALTSIAFALVINFREQTVLVACVCSLLGLFGFAISPVGMELAVECSYPVGEGSSTGLAFISGQIQGIIYMILFQKLTRPFATSGPSPCGMNQTEIYDWSTSMLVMAALCSFGSCIFIIFFHTKYLLALQYILVSVSYLVMAHTMGRYPSAVVVTLVHNVCIAVVCAFVSLLAEKDNPKAWVIRFDITLITVVATGILNSGYYVIHTWAVSHKGPVYLSMFKPLSILIAAVSTFIFLGESLYLGSVMGGILISIGFYMVL",
    "response#3": "RWFVLGVICLLSCTNAMLWISFAPVADVTASFFKCSLDVVNYLSLVYLIIAIPVGFGASWLIDTLGLKYAIVFSSWLNMIGSIIRCGAIVPYLNPSGSYTGIYYLFTGQSLCAIAQPLVLFVPAKLASVWFPEHQRATANMIASMSNPLGVLLANIISPSVVTKEEYIAHMLGIYTVPAIAACILATAGIRAKSPPTPPSASAFNSASEPFIAGIRQLLTNRAYVILMLCFGAGIGIFTAISSFLEQILCFRGYSNLFAGVCGALFIFFGFIGAFVCGLYVDRTKKFKEVVKTCFALTALTSIAFALVINFREQTVLVACVCSLLGLFGFAISPVGMELAVECSYPVGEGSSTGLAFISGQIQGIIYMILFQKLTRPFATSGPSPCGMNQTEIYDWSTSMLVMAALCSFGSCIFIIFFHTKYISKNG"
  },
  {
    "reference": "MPDTASYKQQKEDFVSNLSGGSVAEINYVTSVAAVAILLWSVLQARQSFFEPYTALAFAVDFLLNVGAILLSVTLYSNSPLLLNLLLIAPAILVFTLPPRSRSPKKKAKIPPNARSNESSGQLDILSTKPFLTNFRGCMLIVTCVAILAVDFRLFPRRFAKVETWGTSLMDLGVGSFVFSAGLVAARPVLREKATGRAGAVGNALSLSSRLVQSLRHSIPLLVLGFIRFLSVKGLDYAEHVTEYGVHWNFFFTLGFLPPFVAIFQSVRKLIPSFAALSLLVGVTYQVLLETTSLKAYVLTAPRTDLISMNREGIFSFVGYLAIFLAGQDTGMFVIPRNLVPKSTASPGAQRNKLLKITAVWGGVWTGLYVLSTNYHYGFGLAVSRRMANLPYVLWVVAFNTIQLLGFAVIDTIFFPAFYNAQDAKTEKEAYTHATSRVMRAYNRNGLAVFLLANLLTGLVNMTVNTLDATPIATMGILVVYSAALTGVAVALDAYNISIKL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should contain a Helical membrane-spanning region.2. The protein should be optimized for activity in Glycolipid biosynthesis, glycosylphosphatidylinositol-anchor biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. For general function, the protein need meet that Probable acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor.4. The ideal protein would be one that plays a critical role in GPI anchor biosynthetic process through O-acyltransferase activity within endoplasmic reticulum membrane. The designed protein sequence is ",
    "response#1": "EGLRYLQTYDWLFLRTIVSLGYLGWIAYALTTVIDLHVLHGKSESNRTTFSIMFFSSILVALFSVLLYQGSSWRYYLYALFPIFFWEEVFARRKALLAGREILLGHVHSVSGYFAFAIQLLLYVGVLEALVQSYFHRDIFTVCFILGGFWPITYGTKFLGQHKLLSASWALGCFLMSIFTLLPANKVEDMMMISCGSLLMFLTGLLYLIFERSILGQKRSSDPNSVVSSCGSRTIMGAQVGMILLALIVTRSSVASLQAKQGLPLGNQVLGWAILVSSLLLPFLHRLYPNSHYLHRLMVIFLTFSPIFIILTISYELF",
    "response#2": "EGLRYLQTYDWLFLRTIVSLGYLGWIAYALTTVIDLHVLHGKSESNRTTFSIMFFSSILVALFSVLLYQGSSWRYYLYALFPIFFWEEVFARRKALLAGREILLGHVHSVSGYFAFAIQLLLYVGVLEALVQSYFHRDIFTVCFILGGFWPITYGTKFLGQHKLLSASWALGCFLMSIFTLLPANKVEDMMMISCGSLLMFLTGLLYLIFERSILGQKRSSDPNSVVSSCGSRTIMGAQVGMILLALIVTRSSVASLQAKQGLPLGNQVLGWAILVSSLLLPFLHRLYPNSHYLHRLMVIFLTFSPIFIILTISYEWL",
    "response#3": "EGLRYLQTYDWLFLRTIVSLGYLGWIAYALTTVIDLHVLHGKSESNRTTFSIMFFSSILVALFSVLLYQGSSWRYYLYALFPIFFWEEVFARRKALLAGREILLGHVHSVSGYFAFAIQLLLYVGVLEALVQSYFHRDIFTVCFILGGFWPITYGTKFLGQHKLLSASWALGCFLMSIFTLLPANKVEDMMMISCGSLLMFLTGLLYLIFERSILGQKRSSDPNSVVSSCGSRTIMGAQVGMILLALIVTRSSVASLQAKQGLPLGNQVLGWAILVSSLLLPFLHRLYPNSHYLHRLMVIFLTFSPIFIILTISYEL"
  },
  {
    "reference": "MGLNIGVVGATGAVGRKILDILLERKLDIEELHCFASERTAGEKLDWNGRLVVVELLTEEAMKQPFDYILFSAGGDISLKYAPIAQEAGNTVIDNSSQWRMTDGIPLIVPEVNGHTLRNYQGIIANPNCSTIQMVMALAPLHRIYKMNRIVVSTYQAVSGSGHKAIVELEKQMKDPEYPAQVYPKRIAANCIPHIDVFNENGFTKEELKMVYETHKILSDDSIAINATTVRVPVFYGHSESVTVTFESAPRLMEVKELLRSEPGVVLMDDADNLEYPTPLEIAGTDDTYVGRIRKDLYDPCTLSMWIVADNLRKGAATNAVQIIEKMEELKNQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have NAD binding, NADP binding to facilitate diaminopimelate biosynthetic process, isoleucine biosynthetic process, lysine biosynthetic process via diaminopimelate.2. The designed protein must have at least one Semialdehyde dehydrogenase NAD-binding domain.3. The designed protein should have a binding site that is specific to the target NADP(+) ligand.4. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..5. The designed protein should interact with enzymes and substrates within the L-threonine biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "LTGLHKAYKVKRIVVSTYQSVTGTGKKAVDQLMNERKGVSGDMAYPHRIDMNALPHIDVFLDNGYTKEEMKMVNETRKIMEDDTIRVTATTVRIPVIGGHSEAVNVEFEKEYELADVRRILENSEGVVVMDDPKNNIYPMPITSHNRDEVFVGRLRRDETQPKTLNMWIVSDNLRGRLTVFDSLLKPIHDEYKIKKVVVSTYQSVSGAGILALDELNNQAHDLNTPPKVLPYAAGKKFYPIAYNLLPQIDNFNNETLYTLEEHKMINETHKILSPSIEVIPTCVRVPVKRGHSESLYIECEKEIDIDKARQLLSKMPNVIIQDDLNEQKYPQPIECEDQDYIYIGRIRPALNNKKALCMWVVADNLLQRSDVLGGLFEKDLVEWVSVATYQAASGAGAKNMRELLVQMGELRDVVKTDLANPASSILEIERAVADKMRGDLPIENFGAPLAGSLIPWIDKLLDSGQTKEEWKGYAETNKILGLSDSPIPIDGLCVRIGALRCHSQAFTIKMKKDLPLAEIEQILAAHNEWVKVIPNDKEATLRELTPTKVTGTLSVPVGRLRKLNMGPEYLAAFTVGDQLLKRXDXXTLKPIHDEYKIKKVVVSTYQSVSGAGILALDELNNQAHDLNTPPKVLPYAAGKKFYPIAYNLLPQIDNFNNETLYTLEEHKMINETHKILSPSIEVIPTCVRVPVKRGHSESLYIECEKEIDIDKARQLLSKMPNVIIQDDLNEQKYPQPIECEDQDYIYIGRIRPALNNKKALCMWVVADNL",
    "response#2": "MSPHIAVVGATGAVGTEFLNILKDHYTDLPQLTLLASARSAGNTIEVNGEAVVIQEATPESFEGVDIAFISVSSEISLDLAPHAVKAGAIVIDDSSAFRMNDTVPLVVPEVNGDDCKWLKPLHDAATIKRVVVSTYQSVSGSGKEGMDELFEQTRAVFVADPISAKKFTKRIAFNVIPHIDVFMEDGYTKEEWKVLAETKKMLDPKIKVTATAVRVPVFIGHAESVNLEFEKPITADEARDILREAPGCQVIDKREDGGYITPYESAGEDATYISRIREDATVENGLALWVVSDNLRKGIIANPNCSTTQMVVAMLTGLHKAYKVKRIVVSTYQSVTGTGKKAVDQLMNERKGVSGDMAYPHRIDMNALPHIDVFLDNGYTKEEMKMVNETRKIMEDDTIRVTATTVRIPVIGGHSEAVNVEFEKEYELADVRRILENSEGVVVMDDPKNNIYPMPITSHNRDEVFVGRLRRDETQPKTLNMWIVSDNLRKGAALNAVQIAELLIEKKLV",
    "response#3": "NVAIMGATGAVGQVLLEILAERDFPVGELRLLASARSAGRAISFNGSSIQVQELGEDSFAGVDIVLASAGGSLSKQFNPHAVAAGAVVVDNTSAYRMDPDVPLVVPEINPEDLAKGISKGIIANPNCSTIQMVVALTGLHKAYKVKRIVVSTYQSVTGTGKKAVDQLMNERKGVSGDMAYPHRIDMNALPHIDVFLDNGYTKEEMKMVNETRKIMEDDTIRVTATTVRIPVIGGHSEAVNVEFEKEYELADVRRILENSEGVVVMDDPKNNIYPMPITSHNRDEVFVGRLRRDETQPKTLNMWIVSDNLRKGAALNAVQIAEVLAGR"
  },
  {
    "reference": "MASKSGDGGTMCALEFTVQMSCQSCVDAVHKTLKGAAGVQNVEVQLENQMVLVQTTLPSQEVQALLESTGRQAVLKGMGSSQLKNLGAAVAIMEGSGTVQGVVRFLQLSSELCLIEGTIDGLEPGLHGLHVHQYGDLTKDCSSCGDHFNPDGASHGGPQDTDRHRGDLGNVHAEASGRATFRIEDKQLKVWDVIGRSLVVDEGEDDLGRGGHPLSKVTGNSGKRLACGIIARSAGLFQNPKQICSCDGLTIWEERGRPIAGQGRKDSAQPPAHL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.2. The designed protein must have at least one HMA domain.3. The protein must exhibit the following characteristics: Delivers copper to copper zinc superoxide dismutase (SOD1)..4. The designed protein should be involved in positive regulation of oxidoreductase activity, removal of superoxide radicals by localizing to cytoplasm and exhibiting enzyme binding, copper ion binding. The designed protein sequence is ",
    "response#1": "LNPHSKNHGAPSDEDRHVGDLGNIKTDAQGNSKGSVQDSFIKLIGPHSVIGRTVVVHGGTDDLGKGGHAESLKTGNAGPRPACGVL",
    "response#2": "LNPHSKNHGAPSDEDRHVGDLGNIKTDAQGNSKGSVQDSFIKLIGPHSVIGRTVVVHGGTDDLGKGGHAESLKTGNAGPRPACGVI",
    "response#3": "VEGVVTLTQDDDGPTTVKVRITGLAPGLHGFHLHEFGDTTNGCMSTGPHFNPNGLTHGAPGDEVRHAGDLGNIEANASGVAEATLVDNQIPLSGPNSVVGRALVVHELEDDLGKGGHELSLTTGNAGGRLACGVQ"
  },
  {
    "reference": "MEQDKIRIKLKSYDYKLLDQSVKQIVETVKRTGSSVKGPIPLPTSRRRWVVLRSPHKFDQSREHFEIREFKRMLDIVKITPQTIESLMEISLPAGVDIEVKMRG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein with tRNA binding functions is required to enable translation.2. The protein must exhibit the following characteristics: Involved in the binding of tRNA to the ribosomes.. The designed protein sequence is ",
    "response#1": "MLGAGHIKVRCEDGVERLARIPV",
    "response#2": "MLGAGHIKVRCEDGVERLARIPN",
    "response#3": "MIF"
  },
  {
    "reference": "MSESAFKDCFLTDVSADTRLFHFLARDYIVQEGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIEECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFILLSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a high specificity for [4Fe-4S] cluster binding, with minimal off-target effects.2. A protein that can perform DNA-binding transcription factor activity, metal ion binding, 4 iron, 4 sulfur cluster binding, DNA binding in cytosol to support DNA-templated transcription.3. The presence of [4Fe-4S] cluster is crucial for the protein to function optimally under specific conditions.4. The designed protein should contain one or more HTH crp-type domains that are essential for its function.5. For general function, the protein need meet that Might control expression of the salvage pathways or in some other way repress the recycling of nucleobases to nucleic acids and enhance their use as general nitrogen sources during nitrogen-limited growth.; Transcription regulator involved in mid-term, stationary- phase viability under nitrogen starvation The designed protein sequence is ",
    "response#1": "MALLITSKCTNCDMCLPECPNEAISIGDIKWSKAPCRFCGTGCGVMVGVKEGKVIATHGDMKAEVNRGLNCVKGYFLSKIMYGERLTQPLLRKKNGVYAKDGEFTPVSWDEAFDTMAAKAKQVLKDKGPTALGMFGSGQWTIWEGYTATKLMRAGFRSNNLDPNARHCMASAAYAFMRTFGMDEPMGCYDDFEHADAFVLWGSNMAEMHPILWTRVADRRLGHPHVKVAVLSTFTNRSADLADIPIVFKPGTDLAILNYIANHIITTGRVNQDFVGKHTSFVKGATEIGYGLRPDDPRELKAKKAADPGATTPIDFAAYAAFVKDYTLDKVSALTGVEPGFLEQLAELYADPKRKVVSFWTMGFNQHVRGVWANQLVYNLHLLTGKISEPGNSPFSLTGQPSACGTAREVGTFAHRLPADMTVVNPEHRKHAEEIWRVPAGIIPEKPGYHAVEQDRMLKDGKLNFYWIQVNNNLQAAPNSTGETYPGYRNPENFIVVSDAYPTVTAMAADLILPAAMWVEKEGGYGNAERRTHFWHQLVKAPGEARSDLWQMMEFSKRFTTDEMWPAELLNKHPEYAGQSLYDVLFANDVATDFPTKEATAYGFYIQKGLFEEYASFGRGHGHDLAPFDVYHQARGLRWPVVDGKETLWRYREGYDPYVKAGEGVSFYGYPDKKAIIIGVPYEAPPEAPDKYDRSSARWQLVAPDGKAYNAVRMAPCRFCGTGCGVMVGVKDP",
    "response#2": "TCIGCGRCFKVCSREVMHLHGIDESGEILGACDGEDDDFAGELSRTIMVVDHAGRCIGCGACARVCPKNA",
    "response#3": "FQSYVPIMNGCNNFCTYCIVPYVRGREVSRPVNEILQEITELSSRGVREITLLGQNVNSYKGEDGEGRLIDFPKLLTLIAREADKTDMIRWIRFMSSHPKDMSDALIDTIAAEKRLCKLVHLPVQHGSDTILKRMNRVYTIEHYKNRIKRLKETIPDIALSTDILMGFPGETEDDVKATLDLMQEIEFDSAFMYHYNPREGTKAFNYPDRIPEEVKIERLGRVIPIQKGQELFKAGDELRSLYAIRSGTIKSYTITEQGDEQITAFHLAGDLVGFDAITGDQHPSFAQALETSMVCEIPYEILDDDRLTQPLLRKKNGVYAKDGEFTPVSWDEAFDTMAAKAKQVLKDKGPTALGMFGSGQWTIWEGYTATKLMRAGFRSNNLDPNARHCMASAAYAFMRTFGMDEPMGCYDDFEHADAFVLWGSNMAEMHPILWTRVADRRLGHPHVKVAVLSTFTNRSADLADIPIVFKPGTDLAILNYIANHIITTGRVNQDFVGKHTSFVKGATEIGYGLRPDDPRELKAKKAADPGATTPIDFAAYAAFVKDYTLDKVSALTGVEPGFLEQLAELYADPKRKVVSFWTMGFNQHVRGVWANQLVYNLHLLTGKISEPGNSPFSLTGQPSACGTAREVGTFAHRLPADMTVVNPEHRKHAEEIWRVPAGIIPEKPGYHAVEQDRMLKDGKLNFYWIQVNNNLQAAPNSTGETYPGYRNPENFIVVSDAYPTVTAMAADLILPAAMWVEKEGGYGNAERRTHFWHQLVKAPGEARSDLWQMMEFSK"
  },
  {
    "reference": "MTSTSTTTNGDSFTVKAGLAQMLKGGVIMDVTTPAEARIAEEAGACAVMALERIPSDIRAAGGVARMSNPSMIKEIQAAVTIPVMAKARIGHVTECRILEQLGVDYIDESEVLTPADDTYHVQKDQFKAPFVCGCRNLGEALRRIKEGAAMIRTKGEAGTGDVVEAVKHIRTVNAEIAQAKAALASGGELAVAKMAREIGADVELLKKTAELGRLPVVNFAAGGVATPADAALMMEFGCDGVFVGSGIFKDAKTPEHALQRARAVVKAVANWNDYGALVEACIEHGEAMKGISNAGMKPEERMAGRGW",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in pyridoxine biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.2. The D-ribose 5-phosphate binding site should be located in a region of the protein that is accessible to the ligand.3. We require a protein that can specifically bind to the metabolites produced in the Cofactor biosynthesis pathway. The designed protein sequence is ",
    "response#1": "ELVNFSSNDYLGLSRHPEVVERSRRWLNEYGAGSGASRLVTGHLAAMEALEAKIARCKQTEAALILASGWQCNASVLPALLDKALWGAEPLVFADKLIHASLHAGLELSGARRYRYRHDDLDHLESLLKAHADKEGPRFIVTETVFSMDGDVTDMAALAALASRWDAFLYVDEAHATGVLGANGFGLSPGMGAELAMGTFSKGLGSFGAYVACSARLRHYLINRASGLIYATGLPPAVLGAIDAALDLVPRLEGERTRLQMMGRRLRDGLRAAGLDTGPSASQIVPLILGDEGRTLAVAKALEDRGILGIAIRPPTVPPGTSRIRFALSAVHSDADLDRLLAALA",
    "response#2": "LMPGPALAGLTFAASAEPAEMLATLCRAVYDPRRDDADFRRSLQGDDAQRRAAFDLLRKAYPARREIDGLAVRIEGDNPALTAVVSALGG",
    "response#3": "MPYVQQLFGDLGTIETVNGRELTPEQVKDADVLLVRSVTKVDQALLAENNRLKFVGSATIGTDHIDLDYLASHNIPFSNAPGCNATAVGEFAFIAMLELAQRFNSPLKGKVVGIVGAGNTGTAVVKCLEAYGIEVLLNDPLLEQSGDPRDFVSLDTLIEKCDVISLHVPITKTGEHKTWYLFDEQRLNSLAENTWLVNCCRGEVIDNRALIKFKQQRDDVKVVLDVWEGEPNPMPELVPYVEFCTPHIAGYSLEGKARGTYILYQKP"
  },
  {
    "reference": "MGIEEKLPSGVLLSTVEGLAGYMRKASFWPASFGLACCAIEMMTSGGPKHDLGRFGMEVFRASPRQADVMIVAGRVSQKMAPVLRQIYDQMPEPKWVLAMGVCASSGGMFNNYAVVQGVDHVVPVDMYLPGCPPRPQMLIDAILKLHDKVQHTKMGAHRAAEIEELETAALRALPTSEMKGQLR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein can be expressed in plasma membrane and perform quinone binding, NADH dehydrogenase (ubiquinone) activity, 4 iron, 4 sulfur cluster binding without triggering undesirable cellular responses.2. The protein must be engineered to have a high affinity for [4Fe-4S] cluster in order to increase its catalytic activity.3. The protein must exhibit the following characteristics: The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone; Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient..4. The protein should be able to bind [4Fe-4S] cluster ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "CCAIEGLMAANMARFDLARFGYEVMRASPRQADVMLVCGTVTKKAAPFVVRLYEQMAEPKYVIAMGSCAISGGPFVDSYSVVPGVDKLIPVDVYIPGCAPRPDAVIHGFMKP",
    "response#2": "KVVGIGSPRASLESNFALRELVGEGNFYSGINEGELDRLRLILQVMQEGPLPVPSIRDIEDHDAVFVLGEDLTQTAARIALALRQSVKGKAVEMAADMKVQPWLDAAVKNIAQHAQNPLFIASVSATRLDDVAEETVHAAPDDLARLGFAVAHAIDPSAPSVADLDPQAQAFAQRITDALLAAKRPLVVSGNSLGNKALIEAAANIAKALKQREKNGSISLVVGEANSLGLALFGGDSVEAALERLTSGQADAVVVLENDLYRRTDAARVDAALAAAKVVIVADHQQTATTAKAHLVLPAASFAEGDGTLVSQEGRAQRFFQVFDPTRASVVEWLMTNHPHDCPVCEEGGHCHLQDMTVMTGHNERREWRQPQLKNGNEQLAITVDGALNRAADALRNAKGVIGIGSPRASLESNFALRELVGANNFYVGVEQAEWQCQQQMLHILQHGGVPTPSLRDMEEADAILVLGEDVTMSAARIALSLRQAVKGKARELARKMKVDLWQVAAVQTLGQNQRYPLLITSLDTTRLDDVVEDKLHAPYADQARLGFAIANLLDPAAPAVTDLGAEQQAQAARWAELLGNAKKPLIIAGSSARSMALIEAASNIARALKGRGLEASIALVGQEANSLGLAMLASTSQPLEAALERIEAEEGLALVTVENDLYRRAARNRVDAALARLQHLLVIDHQATSTAQKADLVLPAASFAETDGTLVNMEGRAQRFFQVYAPARQPQLKNGNEQLAITVDGALNRAADALRNAKGVIGIGSPRASLESNFALRELVGANNFYVGVEQAEWQCQQQMLHILQHGGVPTPSLRDMEEADAILVLGEDVTMSAARIALSLRQAVKGKARELARKMKVDLWQVAAVQTLGQNQRYPLLITSLDTTRLDDVVEDKLHAPYADQARLGFAIANLLDPAAPAVTDLGAEQQAQAARWAELLGNAKKPLIIAGSSARSMALIEAASNIARALKGRGLEASIALVGQEANSLGLAMLASTSQPLEAALERIEAEEGLALVTVENDLYRRAARNRVDAALARLQHLLVIDHQATSTAQKADLVLPAASFAETDGTLVNMEGRAQRFFQVYAPA",
    "response#3": "RQPQLKNGNEQLAITVDGALNRAADALRNAKGVIGIGSPRASLESNFALRELVGANNFYVGVEQAEWQCQQQMLHILQHGGVPTPSLRDMEEADAILVLGEDVTMSAARIALSLRQAVKGKARELARKMKVDLWQVAAVQTLGQNQRYPLLITSLDTTRLDDVVEDKLHAPYADQARLGFAIANLLDPAAPAVTDLGAEQQAQAARWAELLGNAKKPLIIAGSSARSMALIEAASNIARALKGRGLEASIALVGQEANSLGLAMLASTSQPLEAALERIEAEEGLALVTVENDLYRRAARNRVDAALARLQHLLVIDHQATSTAQKADLVLPAASFAETDGTLVNMEGRAQRFFQVYAPARASVVEWLMTNHPHDCPVCEEGGHCHLQDMTVMTGHNERRVA"
  },
  {
    "reference": "MQEHQLSRVTSGNKKKYQSFDDESRDEKRMKCDSTDKLESNSNSKNIYQKTHRVIANIRERQRTQALNQSFSTLRKIIPTLPSDKLSKIQTLRLAAMYIDFLRHVIRRGEINMDSSDETFFSAQERLSYAFSVWRMEGDFYSRDKTAHYFTEQELNLCEYNFHSSFGNRLFYKAGIEAVNSADSDDITCILNTFLEGRI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be expressed specifically in the nucleus The designed protein sequence is ",
    "response#1": "LDCEMAFTSLGYEMIRLTIVDFFTGKTLFDHVIQPIGDIVDLNSDFSGVHEIDRTNCPTYKEALDVFLSENLINKNSILIGHGLENDLNVMRLFHNKVIDTAILYSRTKFKVSLKNLAFEVLSRKIQNGEHDSSQDAIATMDC",
    "response#2": "LDCEMAFTSLGYEMIRLTIVDFFTGKTLFDHVIQPIGDIVDLNSDFSGVHEIDRTNCPTYKEALDVFLSENLINKNSILIGHGLENDLNVMRLFHNKVIDTAILYSRTKFKVSLKNLAFEVLSRKIQNGEHDSSQDAIATMDS",
    "response#3": "LDCEMAFTSLGYEMIRLTIVDFFTGKTLFDHVIQPIGDIVDLNSDFSGVHEIDRTNCPTYKEALDVFLSENLINKNSILIGHGLENDLNVMRLFHNKVIDTAILYSRTKFKVSLKNLAFEVLSRKIQNGEHDSSQDAIATMDA"
  },
  {
    "reference": "MSQDRGPRRPRRLEKCALISASATVLSLTASGCVVVHGEREVLPSATRAEAAKALERFTTAYNKADKAYDASLDADHTTGALAAIDSARLKAGAANHPDGNPAHEPLELTDAKFTIPKKAGWPRWFVADTKANKGGTARWLLVFTRDGLEETWEAAYLTLVAPGKVPEFKTDQDGWAEAVPANATDVSVPPAGLSKDYTTYLQDGGKTFADGVHTSSWRALREKRSTRPGLVTQYIDEPLTNGDYAPLALRTEDGGALVFFTSRHFEKQTAARGTSVPTPNKDVLALTDGEIKQSLTMEFVSNEVALDPADGQVSVLGRVQGLTSGKGE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a specific function within the plasma membrane. The designed protein sequence is ",
    "response#1": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPC",
    "response#2": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPC",
    "response#3": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPC"
  },
  {
    "reference": "MSRQVVRSSKFRHVFGQPAKADQCYEDVRVSQTTWDSGFCAVNPKFMALICEASGGGAFLVLPLGKTGRVDKNVPLVCGHTAPVLDIAWCPHNDNVIASGSEDCTVMVWEIPDGGLVLPLREPVVTLEGHTKRVGIVAWHPTAQNVLLSAGCDNVILVWDVGTGAAVLTLGPDVHPDTIYSVDWSRDGALICTSCRDKRVRVIEPRKGTVVAEKERPHEGTRPVHAVFVSEGKILTTGFSRMSERQVALWDTKHLEEPLSLQELDTSSGVLLPFFDPDTNIVYLCGKGDSSIRYFEITSEAPFLHYLSMFSSKESQRGMGYMPKRGLEVNKCEIARFYKLHERKCEPIAMTVPRKSDLFQEDLYPPTAGPDPALTAEEWLSGRDAGPLLISLKDGYVPPKSRELRVNRGLDSARRRATPEPSSTLSSDTVSRLEEDVRNLNAIVQKLQERLDRLEETVQAK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion.2. The designed protein must be able to perform myosin heavy chain binding under specific conditions. The designed protein sequence is ",
    "response#1": "GNPALVSAYGAGLEGGTTGIQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFI",
    "response#2": "GNSSPKDMQDLIHLSGPLTEHAVMRTLQARFNERRYFTNVGPILLSINPYLDVGNPLTLTSTRAMPLAPQLQKIVQEAVRQQSETGYPQAIILSGTSGAGKTANAMLMLRQLFAIAGGGPETDAFKHLAAAFTVLRSLGSAKTTTNSESSRIGQFIEVQVTDGALYRTKIHCYFLDQTRVIRPLPKEKNYHIFYQLLAGLSREERQKLHLDGYSPANLRYLRGDIGQNEQEDAARFQAWKTCLGILGIPFLDVVRVLAAVLLLGNVQFIDGGGLEVDVKGETELNSVASLLGVPPAALFRGLTTRTHNVRGQLVKSVCGDGDANMTRDCLAKALYCRTVATIVRRANSLKRLGSTLGTLSSDSNESVHNQADVASQHASTIGGGNAGSKSMAALNNAVRHATSDGFIGILDMFGFEEPSPHAHLEHLCIV",
    "response#3": "GNSSPKDMQDLIHLSGPLTEHAVMRTLQARFNERRYFTNVGPILLSINPYLDVGNPLTLTSTRAMPLAPQLQKIVQEAVRQQSETGYPQAIILSGTSGAGKTANAMLMLRQLFAIAGGGPETDAFKHLAAAFTVLRSLGSAKTTTNSESSRIGQFIEVQVTDGALYRTKIHCYFLDQTRVIRPLPKEKNYHIFYQLLAGLSREERQKLHLDGYSPANLRYLRGDIGQNEQEDAARFQAWKTCLGILGIPFLDVVRVLAAVLLLGNVQFIDGGGLEVDVKGETELNSVASLLGVPPAALFRGLTTRTHNVRGQLVKSVCGDGDANMTRDCLAKALYCRTVATIVRRANSLKRLGSTLGTLSSDSNESVHNQADVASQHASTIGGGNAGSKSMAALNNAVRHATSDGFIGILDMFGFEEPSPHAHLEHLCIHSAIAIQSWWRGTLGRRK"
  },
  {
    "reference": "MLDSIEEAIQDIKDGKIIIVVDDEDRENEGDLMMAAEKVTSETINFMATNGRGLICMPCVGWRLDELGIPAMVQNNTSEQGTAFTVSIGAKGKITTGISAKDRAVTVQTVVDPNSKPEDISMPGHVFPLRGKKGGVLERAGHTETAVDLAKLAGLFPAGVICEIMNEDGTMARLPQLRKVADKFGLKLVSVEDLIRYRRKTEKLVERVAEVSLPTNFGDFKAIGYRCKLDGKEYFALVKGEVNGQENVLVRVHSECLTGDIFHSLRCDCGDQLEAALRIINAEGKGVILYILGHEGRGIGLLNKLRAYELQDHGRDTVQANNDLGFPTDLRDYGMGAQILADLGLSSIRLMTNNPKKIVGLEGYGLNVVERVPLQVKPTDYNIHYLQTKKEKMHHILENVDRAPEKSSK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Zn(2+) is required for the proper folding and stability of the protein.2. The protein must exhibit GTP binding. The designed protein sequence is ",
    "response#1": "MPELPEVETVRRGLQPHLEGRVIARAEARRPDLRWPLPPDLVQVLTGARVVALRRRSKYILAELEDRGSLLLHLGMSGRMLIEGESQGDFHRDPAILPRHDHVVLWNDQGTRITFNDARRFGMV",
    "response#2": "PELPEVETVRRGLQPHLEGRVIARAEARRPDLRWPLPPDLVQVLTGARVVALRRRSKYILAELEDRGSLLLHLGMSGRMLIEGESQGDFHRDPAILPRHDHVVLWNDQGTRITFNDARRFGMVL",
    "response#3": "PELPEVETVRRGLQPHLEGRVIARAEARRPDLRWPLPPDLVQVLTGARVVALRRRSKYILAELEDRGSLLLHLGMSGRMLIEGESQGDFHRDPAILPRHDHVVLWNDQGTRITFNDARRFGMVL"
  },
  {
    "reference": "MTDTLTITRPDDWHLHVRDGAALEAVVPHSARQFGRALVMPNLRPPVTTAAQAVAYRDRIRAAVPPGTAFEPVMSLYLTDKLPPEEIALAAEAGVRALKLYPAGATTNSDAGVTDIRHTYKTLEAMQKHGLLLLVHGEVTDPAVDLFDREAVFIDRVMIPLRRDFPELKVVFEHLTTQEGAHYVRDANRFTAATITAHHLLYNRNAIFTGGIRPHYYCLPVLKRETHRVALVQAATSGSDRFFLGTDSAPHPAHLKEHALGCAGCYTALTAIELYAEAFDSVDALDKLEGFASFHGPDFYGLPRNSGTITLKRESWTVPETVPYGDATLKPLRGGETVHWKLM",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that is capable of catalyzing reactions within the UMP biosynthesis via de novo pathway pathway with high efficiency and specificity.2. We are looking for a protein with broad substrate specificity for (S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate.3. The designed protein must be optimized for zinc ion binding.4. For general function, the protein need meet that Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate. The designed protein sequence is ",
    "response#1": "DWHLHLRDGAMLEGVLPETTRHFARAIVMPNLVPPVVTGAEAAAYRERIMAALPAGARFEPLMVLYLTETTDPADVRAAAASGLVKAVKLYPAGATTNSASGVRDFDRVRGVLETMAEIGLPLCVHGEVTDPAVDIFDREAVFLERVLEPIRRATPGLRVVLEHVTTRDGLDYVRGGGPDMAGTLTTHHLIINRNHILAGGIRPHYYCLPVAKRETHRLALREAATGGEGCFFLGTDSAPHADAAKESACGCAGETGDRS",
    "response#2": "DWHLHFRDGAMMRGVVPYTARQFARAIVMPNLTPPVTTTALGAAYRERILAAVPEGVDFRPLMTAYLTDETDADDLIDGYTHGVFTAAKLYPANATTNSASGVTDVEALYPVFERMAAAGMVLCIHGEVTDADVDVFDREKEFIERTMAPLHAAIPALKIAFEHITTSDAVDFVVGANDNIGATITPQHLHINRNAMLVGGIQPHNYCLPVAKRETHRLALRQAATSGPPKFFLGTDSAPHEKHTKESACGCAGIFGAPYALESYLAVFEEEDALDKFEAFASLNGPAFYGLE",
    "response#3": "DWHLHFRDGAMMRGVVPYTARQFARAIVMPNLTPPVTTTALGAAYRERILAAVPEGVDFRPLMTAYLTDETDADDLIDGYTHGVFTAAKLYPANATTNSASGVTDVEALYPVFERMAAAGMVLCIHGEVTDADVDVFDREKEFIERTMAPLHAAIPALKIAFEHITTSDAVDFVVGANDNIGATITPQHLHINRNAMLVGGIQPHNYCLPVAKRETHRLALRQAATSGPPKFFLGTDSAPHEKHTKESACGCAGIFGAPYALESYLAVFEEEDALDKFEAFASLNGPAFYGLR"
  },
  {
    "reference": "MSTLQPWISDLRTAVKLGLQGMFKEPPMTAVEWADKHFYMSAESSYNEGRWKTAPFQVAILNAMGNDLIRVVNFVKSARIGYTKLLLANIGYKIQHKRRNVMMWSPTDPDAEDISKSHVNGLIRDVPVMLELAPWFGRKHSDNTLDNKVFANRRNLWIRGGKASRNYREKSPDEVIYDELSKFDADVEGEGSPTFLGDKRLDGAVYPKSIRGSTPGVAGACQITKAAEESPHRLRLHIACPHCQLEQHLKFGGKDCEFGLKWEKNELGEAERAWYVCEHCAACFEHRDMVVAQAKGRWICDETGIWTRDSIDWFGPNNEPIRTPRSVSFYCWAIYSTWTTWVSLVDEWLKVKGDREKLITFINTTRGEVWEEEQGDRVEWQTLYARRENYPKVPPQALVLMGGIDTQDDRYEGRVWAFGLGEEAWLVHRFILTGDPASEELRRKVGLEIHRQFTRADGVPMRVERWCWDAGGHYADEVEAESIKHGVHWVVPTFGASTYGKPIANFPKRRKRKVYKTELGTDNAKELIYSRLRIDVPIPWQPTPGCVHFPIDSDICDEDELKQITAEKKKSVMAKGVRVLRWDSGGRRNEALDCFVYALAALRISQQRFGLDLDQLERVRVDPVPEPVAQQQPSNDNHASTSQGWLNTGSGPWL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The For ATPase activity of the protein must be engineered to enable effective catalysis of Endonucleolytic cleavage of DNA to give specific double-   stranded fragments with terminal 5'-phosphates..2. The presence of Phage terminase large subunit GpA ATPase, Terminase large subunit GpA endonuclease domains is critical for the interaction of the designed protein with its intended targets.3. A protein that can perform ATP binding, metal ion binding, ATP hydrolysis activity, type II site-specific deoxyribonuclease activity, DNA helicase activity in viral terminase, large subunit, host cell cytoplasm to support viral DNA genome packaging.4. The ATP, Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.5. The protein design must feature the Walker A motif, Walker B motif motif.6. The protein requires Mg(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "MMEALSATSRIERVVFMAGGQVGKTECGLNWVGYVIHHAPGPMLLVQPTVEGAKRVSKQRVDALIEASPELASRVKDPRSRDSGNTQLMKEFPGGVLIMTGANSAVGLRSMPVRYLFLDEVDGYPGDADGEGDPVALAVQRAATFVNRKVYLCSTPTLKGFSRIEAAYLESDQRVFEVPCDHCGAHSQIQWRDIKWPTGKMADAAWHCPACDGIHPEYRKPALLANGRYQEGRWETLPYQVAMLNAMGNDEIRIVNVIKSARVGYSKMLLAASAYQIEHKRRHIAFFVPDDGSADLFMKSEIETMIRDVGAVRALAPWCGKKSRDNTLDIKKFSHGKQLWCRGGKAAKNYRAISADTVIYDELAAFDHDIDKEGSPLVLGDKRIEGSTFPKSIRGSTPKLRGPIDRGGCQIEGAVQKSPHLLRYHIPCPHCGAEQYLKWGGKDCAYGIKWDPEQPEDAWYVCEATGCLIRYSEALEAQYKAREAGPWKNTRAPYLMEIMDVLTDRTTRDVTCMMSSQVGKTEILLNAAGYFMHQEPSSIMMVQPTLDNAEAFSKERFDPTIKYTKCLSGLIEEKEENAKKDKRQGSTTMMKYFTGGYAVMVGANSPASLASRPIRVLLMDEIDRYPRSLKKEGDPIKLATQRTTNYHNKKILKISTPTVAGLSRIEDSFELSDKRYFYVPCPHCGEEQTLDWSQVKWLKLDNDLASSKSATLNCKICEKVMRGSGRPDMDWIGQGH",
    "response#2": "MMEALSATSRIERVVFMAGGQVGKTECGLNWVGYVIHHAPGPMLLVQPTVEGAKRVSKQRVDALIEASPELASRVKDPRSRDSGNTQLMKEFPGGVLIMTGANSAVGLRSMPVRYLFLDEVDGYPGDADGEGDPVALAVQRAATFVNRKVYLCSTPTLKGFSRIEAAYLESDQRVFEVPCDHCGAHSQIQWRDIKWPTGKMADAAWHCPACDGIHPEYRKPALLANGREAGPWKNTRAPYLMEIMDVLTDRTTRDVTCMMSSQVGKTEILLNAAGYFMHQEPSSIMMVQPTLDNAEAFSKERFDPTIKYTKCLSGLIEEKEENAKKDKRQGSTTMMKYFTGGYAVMVGANSPASLASRPIRVLLMDEIDRYPRSLKKEGDPIKLATQRTTNYHNKKILKISTPTVAGLSRIEDSFELSDKRYFYVPCPHCGEEQTLDWSQVKWLKLDNDLASSKSATLNCKICEKVMRGSGRPDMDWIGQGHHASYHLNELYSTFRKVPAIVRDYLDKLKTDDLQTFTNVSLARTWEEEGDKVDPDSLMARREEYAAQVPAGGLYITAGIDMQVDRLECEVVAWGVGEESWSLGYHVLWGDPLTPDPWDALDDLLAETYQHETGAIMPIMAACLDTGGTSGYTQAAYDYARGRTGRRLFAIKGVGGWGRPIVEKPQRKQSGKDTRKVDLFLVGTDEAKLITARRLNKTAPGPGYCHFPADRDEEWFKQITAEKLTMRYVKGQPIREWKKPDRARNEALDCRNYALAALKIMQ",
    "response#3": "SAMPGPWRTEHTPYLKGIMDEFNNYETEEIVYVKPTQVGGTECLQNMVGYIVQQDPAPTMIVYPTDTLAKSISENRLQPMFKAAPELRKRFDENSQLQELQFDGMYLTLAGSNSPSSLASKAIRFLFLDEVDKYPGASKKEADPVSLARERTKTFHNRKIFITSTPTLKTGHIWKAKEDADIEKHYFVPCPHCGEYIELKWKQIHFPKEEGMSYADRAEFATYVCQECGCVITDQDKPEMLRKGERGFHLNTLASTFCGWKEIVQKFLVAKEQLDQGNPEGMKVWVNTELGETWEEQGEVVEDAELLGRRELYEADVPDGVLVLTAGIDVQDDRFEVEVVGWGVGKESWGIRYQKIFGDLLKEQVWTDLDAFLLAGFQKKDGTVLHILSACMDSGGHFPDQVLRFTRDRWERKVWAIKGKGGSDVPFIRNPTTNNRVKAPLFTLGVDAGKALLYQRLRHQTKGPNYCHFPINEEAGYDGDYFKGLTAEKMVVRFRKGRPVVTWELKDSKHKRNEPLDLRNYATAALEITKGFHLNTLASTLTTWRDMVEKFIVADEQVKKGNIELMKVWVNTELGETWEEDGEQVEDEELMKRRERYNCEIPEEVLYLTAGVDTQDDRFEVEVVGWGPDYENWGIKYAVIYGDNSNIQNQVWKDLDVFLSQTFHKPDGTKMKIVCTCIDSGGHRSNQVYRFCKERFNRRVFAIRGSNDSAAAYIQKPTKNNREQAYLFTLGVDTGKSLLLQRLKVEEEGPGFCHFPKDEKGKPYSPRGYDEKYFKGLTSEKQVLHYKRGKAVFEWVLKDLGEHKRNEALDCRNYATAAIEIYGM"
  },
  {
    "reference": "MENRWQVMIVWQVDRMRIRTWNSLVKHHMYISKEARNWIYRHHYDSRHPKVSSEVHIPLGEAILVVRTYWGLHTGEKDWQLGHGVSIEWRLRTYSTQIDPDLADQLIHLHYFKCFSDSAIRKALLGEVVRPRCDYPSGHNKVGSLQYLALKALVTPERKRPPLPSVRKLTEDRWNKPQRTKGHRGSHMMHGH",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions; In turn, they are directed to the 26S proteasome for degradation; Finally, Vif also contributes to the G2 cell cycle arrest observed in HIV infected cells.; May interact directly with APOBEC3G mRNA in order to inhibit its translation; Recruits an active E3 ubiquitin ligase complex composed of elongin BC, CUL5, and RBX2 to induce polyubiquitination of APOBEC3G and APOBEC3F; Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism; Seems to play a role in viral morphology by affecting the stability of the viral nucleoprotein core; Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G2. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.3. A protein that localizes to virion component, plasma membrane, cytoplasm and can perform RNA binding with high specificity and efficiency.4. The protein design should include a BC-box-like motif, HCCH motif motif to ensure proper function. The designed protein sequence is ",
    "response#1": "MRVMEIRKNCQHWWRGGILLLGMLMICSATEKAXNXTGXIXGXGMXNXTEVHXEXGXEEXXXFRYKXQLXHPVXXPXAIXYNXTXEXNXKXXXIIXXXTXXXXXTXXXXAXXXHXIXAXAXNXXDXXXXXXXI",
    "response#2": "MENRWQVMIVWQVDRMRINTWKSLVKYHMYVSKKAKGWVYKHHYESTNPRISSEVHIPLGDAKLVVTTYWGLHTGERDWHLGQGVSIEWKQKRYSTQVDPDLADQLIHLHYFDCFSESAIRKAILGQIVSPSCEYQAGHNKVGSLQYLALKALVTPTRTRPPLPSVRKLTEDRWNKPQKTKGHRGSHTMNGH",
    "response#3": "MRVKGIRKNYQHLWRWGTMLFWIIIMCKAEN"
  },
  {
    "reference": "MRGEEEPAVAAAAYTTASKAGLLMELSPPNHESSPPTAGGGGGGGGDGAGGSSSAGASSSAGGGAATPQTPSRYEAQKRRDWNTFGQYLRNHRPPLGLAQCSGAHVLEFLRYLDQFGKTKVHTAACPFFGHPNPPAPCPCPLRQAWGSLDALVGRLRAAFEENGGRPESNPFAVRAVRLYLREVREHQARARGVSYEKKKRKKPQPADTSGGGGHPHPPPPPPPPPSAGAAC",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "DMKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEAN",
    "response#2": "DMKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEAX",
    "response#3": "DMKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEAG"
  },
  {
    "reference": "MADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFSNIQTAINKDQPANPTEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQLKTRSVTHSHSLPDS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be optimized for nucleoplasm, nucleus, mediator complex-specific folding and stability, while maintaining transcription coactivator activity, transcription coregulator activity. The designed protein sequence is ",
    "response#1": "AGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMTALPLRSPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLVICPMR",
    "response#2": "FTEKMQSDMEKIQGKMPSLDVTLDPDTAYPSLILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMTALPLRSPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLVICPV",
    "response#3": "GKYTTIPYRTYICLQGNETSLKEAEWAIRNDNPRALVKSYNKELWTFTLQEGVTPPSALDRFQLTETTTGVFLPSTTTPSSLTMTKQLTPPGYVALMLAIEAMIQEALKRDDHYVPFGNNLILPVTGDLLHLDARLTPGGDLLLQC"
  },
  {
    "reference": "MTTDDLKRLVRTVPDFPSPGILFRDITTLIAHGEGLSACIDHLAHKARAAGAQAVAGMEARGFIFGAAVAARMGLGFIPVRKPGKLPVKTIGIDYALEYGRDRLEIDPTAIREGQRVVIVDDLIATGGTALAAAELLRMAGAHVTHALFVIDLPELHGAERLRGAGLTVEALMDFPGH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Ideal protein should have a binding affinity for key enzymes in the Purine metabolism, AMP from adenine: step 1/1, AMP biosynthesis via salvage pathway pathway to enhance the overall efficiency of the pathway.2. The designed protein should be involved in AMP salvage, purine ribonucleoside salvage by localizing to cytoplasm and exhibiting adenine phosphoribosyltransferase activity.3. For general function, the protein need meet that Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.4. The protein should have a fast turnover rate for AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate +   adenine. The designed protein sequence is ",
    "response#1": "ILEDHKAYTTSIELLVKEFAPYNFDKVAGTEARGFLFGAPLAIELGIGFIPVRKPNKLPRKVISESYDLEYGTDCLEIHEDAVKPGEKVLMLDDLLATGGTMIATANLIRRLPI",
    "response#2": "ILEDHKAYTTSIELLVKEFAPYNFDKVAGTEARGFLFGAPLAIELGIGFIPVRKPNKLPRKVISESYDLEYGTDCLEIHEDAVKPGEKVLMLDDLLATGGTMIATANLIRRLG",
    "response#3": "VIDGLTQRYLENGVDFDVVVGVEARGFILGSALAYTLGKGFVPVRKPGKLPADVVSQEYELEYGTDKVEIHLDAILPGTRVLLVDDLLATGGTALAAAALVEKVGGVVAEMAFIVNLPDIGGERKILDKGYRVLAAEA"
  },
  {
    "reference": "MAADIIFHRPVARWTSLYLEEGGIRLGTNPQENVNNRFLNSVDVVVNWARKYSLWPMFFGLSCCFVEEAAAFTSRYDLARFGAEVLRPSPRQADLLIVSGTVFKKIAPIVLRLYEQMAEPKWVISMGSCSNCGGMYDVYSVVQGIDQILPVDIYIPGCPPRPEAVLQGLLLLQEKITSEERPARSIFHLPGGTQGSRRPIRVDGLTKSRDPRGPGMEGIPIRGSSVIPPLFRDSRSDLMWTPPARRIELKDEERVLADILKDRFGEAIERVPQTADMPTFNVAENRIKDVLRFLKNDATPRFQRLEDLTAIDESARRNRSAYPDFTLVYHLLSFDAASRLRLKVPLHGEAPSAQTATDIWPSANWYEREVFDMFGIRFDGHPNLRRILMPHDWQGYPLRKIHPGRATEMPPYALADARKHQPLDGGIFVKHADGEQKLVLNMGPHHVSTHGLLRYVVSLDGEEITGLDMDIGYHHRGVEKIGERQSWHQFIPYCARVDYLAGAANDLPYVLAVETLADIKVPERAQVIRVMLSELFRLSNHLVWFATYAHDVGAMTPNFYTFREREIILDIVELITGGRLHPSWFRLGGVAADLPEGWKDAIDHFVKIFPARLREYESLIRKNPIFKARTEGIGRLSLEDAMDWGVTGPNLRACGLEWDLRKKIPYSGYENFEFEVPTASGGDCYARYLVRVEEMRQSLKIIEQAAANMPEGRYVTDDYRYVIPKRTDMLKDIESLIHHFINVTRGPKIPRGEAYVSCEIPRGEQGYY",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The [4Fe-4S] cluster binding site should be stable and able to withstand changes in environmental conditions.2. The designed protein must have at least one NADH-quinone oxidoreductase subunit D, NADH:ubiquinone oxidoreductase-like 20kDa subunit domain.3. The designed protein must be able to perform iron ion binding under specific conditions.4. The protein must bind to [4Fe-4S] cluster in order to perform its enzymatic function.5. The protein should have a fast turnover rate for a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) +   NAD(+).6. The protein must exhibit the following characteristics: The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. The designed protein sequence is ",
    "response#1": "DVGAMTPNFYTFREREKLLDIVEFITGGRLHPSWFRIGGVAQDLPDGWKEAVAAFVLEFPGRLKEYEELITRNPIFRARTLGVGRISLQDALEWGVSGPNLRACGMAWDLRKAFPYCGYEAFDFEVPTATDGDCYARYLVRVEEMRQSLRIIEQAARNMPSGRWVSEEYRYCIPQRKDSLQDIESLIHHFINVTQGPRLPQGEAYAATECPRGEQGYYVVSEGLGYPYRVRIRAPGFANMQVMPLLAKGDIGAMTPVFYTFTDREKIFDIVEMITGGRMHPAWFRIGGVAEDLPDDWERPVRDFLAWMPARLKEYHSLLDKNPIFMARLKGVGAISKDDALEWGLSGPNLRACGMEWDLRRKIPYSGYDRFDFDIPTADGGDCWARYLVRMEEIQQSLRIVEQCFTTMPAGRWMTDDYRYVLPQKPDTLKDIESLIHHFVNSTRGMSAPKGENYSAIEAPKGENGYFVVSDGLNIPYRVRIKTPSFPHVQALPVMSRRG",
    "response#2": "DVGAMTPNFYTFREREKLLDIVEFITGGRLHPSWFRIGGVAQDLPDGWKEAVAAFVLEFPGRLKEYEELITRNPIFRARTLGVGRISLQDALEWGVSGPNLRACGMAWDLRKAFPYCGYEAFDFEVPTATDGDCYARYLVRVEEMRQSLRIIEQAARNMPSGRWVSEEYRYCIPQRKDSLQDIESLIHHFINVTQGPRLPQGEAYAATECPRGEQGYYVVSEGLGYPYRVRIRAPGFANMQVMPLLAKGDVGAMTPVFYTFTDREKIYDIVEMITGGRMHPAWFRIGGVADDLPEGWEAPVAEFLRWFPPRLREYEGLLNDNPIFTARLKGVGTISREDAIEWGISGPNLRACGMEWDLRKKMPYCGYDRFDFDIAVAEGGDCYARYLVRIEEMKQSLRIVEQAYRGMPGGRWISEQYRYTLPQKRDTLEDIESLIHHFINTTRGMAPPKGECYAPIEGAKGEYGYFVVSDGINIPYRVRIRTPGFAHMQALPLQ",
    "response#3": "CFVEEATVITSRYDIARFGAEVFRPSPRQADLLIVSGTVFKKIAPVVLRLYEQMPEPKWVISMGSCSNTGGMYDVYSVVQGVNQILPVDVYIPGCPPRPEAVLQGLVEKAS"
  },
  {
    "reference": "MQPWLWLVFSMKLAALWSSSALIQTPSSLLVQTNHTAKMSCEVKSISKLTSIYWLRERQDPKDKYFEFLASWSSSKGVLYGESVDKKRNIILESSDSRRPFLSIMNVKPEDSDFYFCATVGSPKMVFGTGTKLTVVDVLPTTAPTKKTTLKMKKKKQCPFPHPETQKGLTCSLTTLSLLVVCILLLLAFLGVAVYFYCVRRRARIHFMKQFHK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that contributes to adaptive immune response, T cell activation by functioning in external side of plasma membrane, receptor complex with MHC class I protein binding, coreceptor activity.2. The protein must exhibit the following characteristics: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses; Additionally, plays a critical role in thymic selection of CD8+ T- cells..3. The protein should have Helical domains to ensure proper insertion into the membrane.4. The Extracellular topological domain of the protein should be designed to be flexible or rigid.5. The designed protein should contain one or more Ig-like V-type domains that are essential for its function.6. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "MLLLPLLLLAVIVPGGDNEDVFQGPTSFHVIQISTFANSTWAQNQGSGWLDNLQLYGWDSDPGTTIFLKPWSKGNFSDEEVTELEELFRVYLIGFTLEVQDHVSEFQLEYPFVIQDIAGCELHPGKAVESFLKGAFGGLDFVSIKNDSCAPVPEGGSMAQRFYELIIQYHAICDTIAKLLLETCPRYFLSVLDAGKAC",
    "response#2": "MLLLPLLLLAVIVPGGDNEDVFQGPTSFHVIQISTFANSTWAQNQGSGWLDNLQLYGWDSDPGTTIFLKPWSKGNFSDEEVTELEELFRVYLIGFTLEVQDHVSEFQLEYPFVIQDIAGCELHPGKAVESFLKGAFGGLDFVSIKNDSCAPVPEGGSMAQRFYELIIQYHAICDTIAKLLLETCPRYFLSVLDAGKAC",
    "response#3": "MLLLPLLLLAVIVPGGDNEDVFQGPTSFHVIQISTFANSTWAQNQGSGWLDNLQLYGWDSDPGTTIFLKPWSKGNFSDEEVTELEELFRVYLIGFTLEVQDHVSEFQLEYPFVIQDIAGCELHPGKAVESFLKGAFGGLDFVSIKNDSCAPVPEGGSMAQRFYELIIQYHAICDTIAKLLLETCPRYFLSVLDAGKAC"
  },
  {
    "reference": "MNKKLEQLEKFKTNDKNPVYSTTNTGVSLSDDANSLKAGPRGPTLLEDFVLREKITHFDHERIPERIVHARGTGAHGYFLSYKDHSKLTKADFLSKQDKKTPVFIRISTVQGPRGSADTVRDVHGFAVKFYTDEGNYDLVGNNMPVFFIQDASSFPDFVHAVKMEPQNEMPTGGSAHDTFYDFCGLKPESAHSVLWVMSDRGIPISLRHQQGFGVHSYRFINQEGKSTFVKLHWKPLSGTCSLLWDEAQKIAGKDCDYHRRRFWEDIESGDFPQWELGAQLLDEDLQKKFDFDILDPTKLIPEELTPVIPLGRMVIDRNPDNFFAETEQVAFCVSHVVPGIDFSNDPLLQGRIFSYLDTQLSRLGGPNFNEIPINRPVCPFANNQRDGIHRMTINKGGASYFPNSIDKGYPLLKEPSANKGGFRPYPENISGTKSYDRSETFEDHFSQATMFWNSMSQHEKNHIIAAYTFEISKCSRPEVRTRYVNNILVNIDSVLAEKVAKNLGVKIEPTSTKPIKKIMVKPSPALSQPNLLSGDIVSRRISVIIEKGVDYDDVINFKDDMEKRGAMVMLVSSTLAQVECSGGEMLSPKGTIIGNPSIFFDAVYVPKSTEEATKILSDDGNFLHYILEAFKHLKTIAFGGSVSVIKELLRLPQDHGLLLGNGYKDITEQFFYSLAHHRVWERESKVSKIPA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Required for normal developmental timing, possibly through a regulatory role in differentiation and morphogenesis..2. heme is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "YPIFAQQGYENPREATGRIVCANCHLAKKPVNIEVPQSVLPDTVFEAVVQIPCDMQIKQVLANGKKGALNVGAVLILPEGFELAPPDRISPEIKEKIGNLYFQNYRPNQKEILVIGPVPGQKYSEIVFPILSPNPATNKEVHFLKYPIYVGGNRG",
    "response#2": "HGPVPKGTWDDWTVEVTGLVKRPMKFTMDQLVNEFPCRELPVTLVCAGNRRKEQNMVKQTIGFNWGAAAVSTTIWRGVPLRALLKRCGVFSKNKGALNVCFEGADVLPGGGGSKYGTSIKKEFAMDPARDIIVAYMQNGEKLAPDHGFPVRMIIPGFIGGRMVKWIKRIIVTTQESDSYGETVALVAGGHTFGKAHY",
    "response#3": "HGPVPKGTWDDWTVEVTGLVKRPMKFTMDQLVNEFPCRELPVTLVCAGNRRKEQNMVKQTIGFNWGAAAVSTTIWRGVPLRALLKRCGVFSKNKGALNVCFEGADVLPGGGGSKYGTSIKKEFAMDPARDIIVAYMQNGEKLAPDHGFPVRMIIPGFIGGRMVKWIKRIIVTTQESDSYGAAE"
  },
  {
    "reference": "MELVDPNLEPWKHPGSKPTTECSNCYCKKCCWHCQLCFLKKGLGISHGRKKRKHRRGTSQNRKDHQHPLPKQPPSNSRGNPTGQKESEKKVASKAETDH",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should emphasize Basic and acidic residues, Basic residues compositional bias.2. For general function, the protein need meet that This binding increases Tat's affinity for a hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA) and allows Tat/P-TEFb complex to bind cooperatively to TAR RNA; Through its interaction with host TBP, Tat may also modulate transcription initiation; Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation; Tat can reactivate a latently infected cell by penetrating in it and transactivating its LTR promoter; To ensure maximal activation of the LTR, Tat mediates nuclear translocation of NF-kappa-B by interacting with host RELA; Besides its effect on RNA Pol II processivity, Tat induces chromatin remodeling of proviral genes by recruiting the histone acetyltransferases (HATs) CREBBP, EP300 and PCAF to the chromatin3. A protein that can perform protein serine/threonine phosphatase inhibitor activity, RNA-binding transcription regulator activity, cyclin binding in host cell nucleolus, host cell cytoplasm to support suppression by virus of host type I interferon-mediated signaling pathway, negative regulation of peptidyl-threonine phosphorylation, modulation by virus of host chromatin organization. The designed protein sequence is ",
    "response#1": "MENRWQVMIVWQVDRMRIRTWNSLVKYHMYRSKKAKGWFYRHHYESRHPKVSSEVHIPLGDARLVITTYWGLHTGERDWHLGQGVSIEWRQGRYITQVEPDLADQLIHLYYFDCFADSAIRKAILGHIVSPRCEYQAGHNKVGSLQYLALTALVAPKRIKPPLPSVRKLTEDRWNKPQKTKGHRGNHTMNGH",
    "response#2": "MEPVDPNLEPWNHPGSQPKTPCNKCYCKKCCFHCYMCFIKKGLGISYGRKKRRQRRRAPPSSEDHQNLISKQPIPQTRGNPTGPKESKKKVESKTETDPCD",
    "response#3": "MENRWQVMIVWQVDRMRIRAWKSLVKHHMYISKKAKDWFYRHHFDSRHPKISSEVHIPLGDAKLVITTYWGLHTGERDWHLGQGVSIEWRQKRYSTQVDPGLADQLIHIHYFDCFSESAIRKAILGHRVSPRCEYQAGHNKVGSLQYLALTALIAPKKIKPPLPSVKKLTEDRWNKPQKTKGRRGSHTMNGH"
  },
  {
    "reference": "MTLSGKRILLIIGGGIAAYKCLDLIRRLRERGASVRCVMTSAAQEFITPLAVGALSADHVFTDLFDRQDEHDIGHIRLSREADLLVVAPATADLMAKMANGLANDLASTVLMATDKKVLVAPAMNPRMWAHPATARNRTTLEADGIAFVGPARGEMAESGEAGEGRMAEPLDIVAAIEGRLAPSARPLAGRRIVVTSGPTHEPIDPVRYIANRSSGKQGHAIAAALARLGADVRLVAGPVNVPDPAGVKVAHVETARQMKDAVEAALPADAGVFVAAVADWRAEGETGQKIKKEEGKAPPALQMVENPDILATIGHHPQRPGLIVGFAAETQNLVDNARRKLAKKGADIIVANDVSHDSGVNATGVMGGDRNLVRIVSADGVEEWPEMTKDEVAARLAALIAERLTGE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be optimized for activity in Cofactor biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.3. The protein should be designed to have high stability and activity towards H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. The designed protein must be optimized for phosphopantothenoylcysteine decarboxylase activity, metal ion binding.5. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.6. The designed protein have Flavoprotein domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "KKILLGVTGGIAAFKAASLCSKLVQQGAEVRVIMTESATKFITELTLQSLSKNPVYTDTFDEKNPAAIAHINLADWADLVLIAPATANIIGKMAAGIADDMLSTTLLATQAPIMLAPAMNVHMYVHPAVSRNLAELAARGVMLVEPGEGLLACGYTGKGRMEEPESIVAVVLAAVAGDLGGGGLLSGKRVLVTAGPTYEDIDPVRYLGNRSSGKMGFELAKAAYLRGAEVLLISGPTSERAYQEIPIIRVRSAAEMKTVVDKSVKNSDILIMAAAVADFKPSKKSENKIKKEENPESIRLEKTEDILTGLKTKAKSGETVKVGFALETENEEKNAIKKLKGKGLDLIVLNSLRDEKSGFEYDTNKIKIIDKSGIEEWPEMSKEAVAEHLVARIAEKLG",
    "response#2": "KKIILGISGSIAAYKSAILTRLLIKAGAEVQILMTPSAQSFITPLTLSTLSKRPVFSEVSSESGWNNHVELGLWADVMVIAPVTATTLAKLANGICDNILVAAYLSARCPVFFAPAMDLDMWHHPASRNNVQRLQGYGNHLIPVGVGELASGLSGEGRMAEPEEIVAYLETHLRYQPLQGKNVLVTAGPTVAYIDPVRYITNRSSGKMGYSIAKEARDRGAKVTLVSGPTKLKAPLGVDLIKINTNEEMLKAVKENFKNQHIVIKSAAVADYKPKEYKEHKIKKIGDDLNLELIKDKDILKELGLIKEDQILVGFAAESRDLLDNAKSKLEKKNLDFIVANNILSKETGFASDDNVVTILTANEQSHWPRMSKETVAERLVFHISNLFSS",
    "response#3": "RILLIVSGSVAAYKALELTRLLRKAGHEVSPILTAGGARFVTKEALAGISGARVHDDLWAAEAEIGHIRLARWPDLVVVAPASANMIARMANGLADDLAGTVLLATRAPVLVAPAMNPAMWAHPATVANMAALRSRGVAVAGPEEGAMAEPEGRLAEPALLKHILLAVSGGIAAYKSAELVRLLRKNGAEVRVVMTQAATQFVSTLTFQALSGHAVHTDLLDAEAEHAMDHISLARWADALLIAPASANTIAKCSWGLADDLLSTLYLAATCPVYVAPAMNQAMWHHPATQENIAKLKNHGVIVIGPEPGEQACGETGLGRMSEPAVICEQILALLAPRAAPLSGKKVVVTAGGTVERIDPVRYISNDSSGKMGFALAKAAHELGADVTLLAANTHLPLPAGIRVVHLESAQEMYDAVLAEWSVTDILVKAAAVADYRAKEVSDRKIKKSGETLTLELVKNIDILESLGKQKTKQLLIGFAAETNDLEKYAMDKLQRKNCDLLIANDVTREGAGFGTDTNLVNLLGADGSEHSIEHGSKLEMAHVLLDRVAALL"
  },
  {
    "reference": "MKIKLNTIEEAIEDIKAGKVVIVVDDEDRENEGDFVTAARNASPEIINFMATHGRGLVCAPLTEERCRELNLGLMVNNNTAVYETNFTVSVDLQGYGCTTGISASDRSKTIKALIDPNIRPEELGRPGHIFPLIAKDGGVLRRTGHTEASVDLARLAGFEPAGVLVEILKDDGEMARLPDLIKVAEKFDLKIISIEDLIEYRLKHDTLIKEEVSVEMPTEWGLFKMKAFTQKNTGEQHLALYKGEWTEDEPILVRVHSSCVTGDIFGSCRCDCGPQLHKAMEMIEKEGKGIVVYMNQEGRGIGLVNKLHAYKLQEEGIDTVDANTQLGFKPDLRDYGVGAQILRYMGVTKMRLMSNNPTKRAGLIGYGLEIVENVPIEIHPNEYNEKYLKTKRDRMGHTLSNL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should contain one or more GTP cyclohydrolase II domains that are essential for its function.2. The designed protein is the optimization of the Nucleophile; for GTP cyclohydrolase activity, Proton acceptor; for GTP cyclohydrolase activity to achieve high catalytic efficiency for GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.3. A protein with GTP binding, GTP cyclohydrolase II activity, magnesium ion binding, zinc ion binding functions is required to enable riboflavin biosynthetic process.4. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation.5. The designed protein should be able to compete with endogenous proteins or inhibitors for GTP, D-ribulose 5-phosphate, Mg(2+) binding.6. For general function, the protein need meet that Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.7. We require a protein that can specifically bind to the metabolites produced in the Cofactor biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4, riboflavin biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MKLNAFLPTKFGDFKIILYHSETDSKDHVALVKGEISPDKPTLVRVHSECLTGDVFGSLRCDCNDQLQSALKIINEAGSGVVLYMRQEGRGIGLSGKIRAYRLQDEGLDTVEANEKLGFRADLRDYGIGAQILLDLGVRKIRLLTNNPKKIVGLEGYGLEIVERIPAPALPDGADQREVEINLPTSYGDFRTIAYTNQIDGREHIALVKGTISPEEPTLVRVHSECLTGDVFGSHRCDCGPQLESALQQIEEAGKGVLLYMRQEGRGIGLLNKLKAYKLQEEGYDTVEANEKLGYAADLRDYGIGAQILEDLGVRKMKLLTNNPRKIKGLHGYDLELVDRVPHAELPDVDLREVVAQLPSEFGDFRIAAYTNLLDGKEHVALIKGEISSDEPILVRVHSECLTGDVFGSLRCDCGPQLHAALEQIEQAGSGVLLYLRQEGRGIGLINKLRAYNLQEQGFDTVEANEKLGFAADLREYGIGAQMLRDLGVRGMRLMTNNPRKIRGLEGYGLKVIERVPAPALPDGADQ",
    "response#2": "GVDMPTEWGHFDLIAYRQTNTDELHLALVKGTWDKDEPVLVRVHSSCVTGDIFGSCRCDCGGQLHAAMEMVDKAGKGVVLYMNQEGRGIGLLNKLKAYKLQEMGRDTVDANLELGLPMDSRDYGVGAQILRDLGITKINLISNNPKKRVGLMGYGLEIVDSVPATEVPDVPDEAEAKLPTKYGCFKVVGYRNKINGKENLAIVKGNIQNEKEVLVRVHSKCLTGDVFGSLRCDCGNQLSAALKAIEKHGSGVLLYMDQEGRDIGLLNKLKAYELQEKGMDTVEANLELGFPPDLRDYWGAAEILKDLGVKRIKLMTNNPDKIEKLQKYGIDVVKRVEAEAKLPTKYGCFKVVGYRNKINGKENLAIVKGNIQNEKEVLVRVHSKCLTGDVFGSLRCDCGNQLSAALKAIEKHGSGVLLYMDQEGRDIGLLNKLKAYELQEKGMDTVEANLELGFPPDLRDYWGAAEILKDLGVKRIKLMTNNPDKIEKLQKYGIDVVKRVPELPREDLERVAEATLPTKYGGEFKIIVYENEVDDMKHIALVKGDIQPEDEVLVRVHSECVTGDLFGSLRCDCGDQLHKAMEMVGQEGKGVIVYMHQEGRGIGLANKIRAYDLQEQGRDTVEANIELGFKEDLRDYGIGAQILVDLGVRKMRMITNNPKKIVGVEGYGLTVVERVPELPRDDLE",
    "response#3": "MSNAFLPTKFGDFKIILYHSETDSKDHVALVKGEISPDKPTLVRVHSECLTGDVFGSLRCDCNDQLQSALKIINEAGSGVVLYMRQEGRGIGLSGKIRAYRLQDEGLDTVEANEKLGFRADLRDYGIGAQILLDLGVRKIRLLTNNPKKIVGLEGYGLEIVERIPREEVVVPESPVAKLPTRYGEFKIAAYENIINGEHHVALIKGEIESSEEPVLVRVHSECLTGDAFHSERCDCGEQLDAAIKQISEEGRGVLLYMRQEGRGIGLVNKIRAYELQDKGKDTVEANVLLGFAPDLREYGTGAQILYDLGVRKIRLLTNNPKKIVGLSGYGLEIVERVPLYHPRPPKERVTQAHMPTKYGNFKMVGYVNKVNGEHHIALVKGDITDGEPVLMRVHSECLTGDAFGSLRCDCGDQFAAAMKKINEEGRGLLLYMRQEGRGIGLINKIKAYALQDTGMDTVEANLALGFSEDMRDYGIGAEIIRDLGIKKIKLMTNNPKKINGLEGYGLEIVERVPLYHPRPPKERVTQAHMPTKYGNFKMVGYVNKVNGEHHIALVKGDITDGEPVLMRVHSECLTGDAFGSLRCDCGDQFAAAMKKINEEGRGLLLYMRQEGRGIGLINKIKAYALQDTGMDTVEANLALGFSEDMRDYGIGAEIIRDLGIKKIKLMTNNPKKINGLEGYGLEIVERVPLYHPRPPK"
  },
  {
    "reference": "MKYNREEFEEVIVNIGRVTKVVKGGRKFRFTALVVVGNRKGLVGYGFGKSKEVPDAIKKAVDDAFKNIIDVKLNGSTIAHDIEVKYNASKILLKPASEGTGVIAGGSVRPVLELVGIKDILTKSLGSNNSSNVVRATIKALSMLKN",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design must be focused on enhancing the efficiency of translation.2. For general function, the protein need meet that Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body. The designed protein sequence is ",
    "response#1": "AVTLEKGYHFRRAAFMTIRRIMNAGALGAEVIVSGKITSERAKFEKLKEGIVYKTGNNLDVMVDRAIAIATLKMGIYGVEVVS",
    "response#2": "MSPLKF",
    "response#3": "AVTLEKGYHFRRAAFMTIRRIMNAGALGAEVIVSGKITSERAKFEKLKEGIVYKTGNNLDVMVDRAIAIATLKMGIYGVEVV"
  },
  {
    "reference": "MSKAFVITMDGPAGVGKSTLARRLAAELGIAYLDTGAMYRTLGLALGAEAADLPEEELRRRCAAFAFSLRRGEGGSWILCCCGRPVGEEIRTERAGRLASLVARLPVLRSELQKAQQELGEGVSLVAEGRDMGTKVFPGARRKFFLDARPEVRAERRFRELLARGLEADFNTVLNDIVQRDEQDRGRALDPLRPAPDAVLVDTSDLDPDAVLRVLLNSVHAALGRDGLRHEAEPGFTHLAEDGSLRMVDVGGKLETRRVARARAVVEMSASTLDLLRRDALPKGDVLAVAKVAGIMAAKRTWELIPLCHPLAVNYADIRFEIRDEPPSVLLESEVRVSGRTGVEMEALMAAQVAAATIYDMAKAVQKDMVIRDVRLVYKSGGKSGDFRAE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein localizes to the cytoplasm. The designed protein sequence is ",
    "response#1": "RRRRRGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS",
    "response#2": "CCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGK",
    "response#3": "RRRRRGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS"
  },
  {
    "reference": "MSTDFDRIFMNQSKFGGRFRIADSGLGWKVSTSGGSASAQNKAPFLLPATELSTVQWSRGCRGFELKINTKNQGVIQLEGFSEDDFNIIKGDFHRRFSIQVEHKEHSLRGWNWGQTDLARNEMVFALNGKPVFEIPYARINNTNLTAKNEVAVEFNIQDDTYQPAGDEMVEMRFYLPGSVVVDEDQPAPKKEGEEEGEEAAETETKSLAEAFYEELKNKADIGEIAGDAIVSFQDVFFTTPRGRYDIDIYENSIRLRGKTYEYKLQHNQIQRIVSLPKADDINHLVVLAMDPPLRQGQTTYPFLVLQFQKDEETEVQLNLSDQEYEEKYKDKLKKQYDSKTHIVISHVLKGLTGRRVVVPGEYKSKYEQCAVSCSYKANEGYLYPLDNAFFFLTKPTLYIPFNDVSSVVISRAGQTSTSSRTFDLEVILRSNRGSTIFGNISKEEQQLLENFLKSKNLRVKNEEKDAQVRLQSALGSDSDDEDVNMGSAGEDDESVDEDFHVSSGDDDDEVAEEFDSEAASEGEDEDEDMDGSDRPTKKPKTE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be expressed specifically in the FACT complex The designed protein sequence is ",
    "response#1": "VLMVGPPGTGKTLLAKAVATECGTTFFNVSSAGLTSKWHGESEKLVKLLFDMARFYAPSTIFIDEIDSIGSKRGSGGEHDATKRLTSEILIQMDGVAGAAGGDVEVDAETGESKVVMVLGATNFPWNLDNALLRRLEKRVYIPL",
    "response#2": "VLMVGPPGTGKTLLAKAVATECGTTFFNVSSAGLTSKWHGESEKLVKLLFDMARFYAPSTIFIDEIDSIGSKRGSGGEHDATKRLTSEILIQMDGVAGAAGGDVEVDAETGESKVVMVLGATNFPWNLDNALLRRLEKRVYIPL",
    "response#3": "PLKGVLMVGPPGTGKTLLAKAVATECGTTFFNVSSAGLTSKWHGESEKLVKLLFDMARFYAPSTIFIDEIDSIGSKRGSGGEHDATKRLTSEILIQMDGVAGAAGGDVEVDAETGESKVVMVLGATNFPWNLDNALLRRLEKRVYIPLPT"
  },
  {
    "reference": "MRKDADFHAPTPAGESAAAPVRTPDGGDRPVLSLLRFITCGSVDDGKSTLIGRLLHDTGQIREDQVDALRRDSRRFGTQGERLDLALLTDGLAAEREQGITIDVAYRFFSTARRRFIVADCPGHEQYTRNMVTGASTAAAAVLLVDARHGVATQTRRHAYLCHLLGIRQVILAVNKMDLVAFDRAAHEAVDAQFRAFADALGIAHITSVPISALAGDNVLTPSPRLGWHDGPTLVGALEAAEPDDARGLAGPFRMPVQAVHRAPAGERSFAGLVASGTARRGERVTVLPSGASTTVAQILSGFASVDETAAGQSVALVLADPVDCSRGSVICAAGTAPEHAAAFEATLVWMDEEPLLPGRAYTMKLATQTVSVRVQEPRHEVNINTLEHLAARTLRRNSIGVAEILCDQPVLFEPYATSRALGGFILVHPANGRTVGAGMITAPLRRTQNVQWQPLAVTRQTRASLKRQRPRVLWFTGLSGAGKSTIANAVEERLVAAGHHTFLLDGDNIRQGLNRDLGFGDADRVENIRRIGEVARLMTDAGLIVLAAFISPFRAERDMVRSLLPPGEFVEIFVDTPLAVAEARDVKGLYRKARAGEIANFTGISSPYEPPLAPELTVDTTSLSPEEAADLVIERLKPF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design must be able to perform adenylylsulfate kinase activity, sulfate adenylyltransferase (ATP) activity, GTPase activity, GTP binding in order to contribute to sulfate assimilation, hydrogen sulfide biosynthetic process, phosphorylation.2. The presence of Tr-type G domains is critical for the interaction of the designed protein with its intended targets.3. A key design requirement for the protein is the optimization of the Phosphoserine intermediate to achieve high catalytic efficiency for adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate   + ADP + H(+).4. For general function, the protein need meet that Proposed to provide activated sulfate for transfer to Nod factor; ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity.5. The protein should be optimized for activity in sulfite from sulfate: step 2/3, hydrogen sulfide biosynthesis, Sulfur metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.6. The designed protein should have a high specificity for ATP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "TMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGQDIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTKRHSYLAHLIGIRNIVLAVNKMDLVDYSQAAFDKIVADYREFAHSIGIENFVAMPISGFRGDNITALSFRMPVQWVNRPNLDFRGFSGLISDGEIAPGDTVRIVPSGKTSTVKSIVTMDGDKDEAVAGQSVTLTLADEVDCSRGDVIGAGDQFEATLIWMDEAPLVPGRQYLMKIGTQTVPATLHAPKYRLDVETLAHLAAKTLALNDIGVANLWTARPILFEPYEKSRDLGGFILIDRITNATVAAGLEIADRFSAHLIWMGDQKLVPGRTYAVKAGKQSATGVVSRIGHRIDVNTFAQDEVDSLSLNEIGFCNLALSAPIAFDPYEVNREMGSFILIDRMTNQTVAAGVVNHSLRRADNIHWQATEVTRAQRAQQKGQSPCVVVAVNKMDQVADQFEATVVWMADDELIPGRAYWLKLGTQMVQATVQAPKYVVNVNTMEHLAAKTLDLNAIGVCELTTDKPIVFEPYTDNRTLGGFILIDRISNATVAAGLLDHGLRKSENLHWQKMDVTPAARAAVKGENAARLITCGSVDDGKSFDATVCWMADESALEPGRDYILKHTTRTTRVRITDLDYRLDVNTLERDKSATALKLNELGRISLRSQAPLLLDEYTRNPATGSFILIDPVTNGTVGAGMLNHGMR",
    "response#2": "TMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGQDIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTKRHSYLAHLIGIRNIVLAVNKMDLVDYSQAAFDKIVADYREFAHSIGIENFVAMPISGFRGDNITALSEVSDQFQATMLWMSDSAMLPGRSYVMKMGARECQMQVTEERYRIDVNTRAHEAAKTLGLNEIGVVNIALDREVAFDPYNDNRRMGGFIVIDRMTNETVGLGLFRMPVQWVNRPNLDFRGFAGLIGSGMVSPGDRIRVLPSGTESTVERIVTMEGDLELAGAGRSVTLTLSDEIDISRGDSIVHPGQVADQFEATVVWMADDELIPGRAYWLKLGTQMVQATVQAPKYVVNVNTMEHLAAKTLDLNAIGVCELTTDKPIVFEPYTDNRTLGGFILIDRISNATVAAGMLNFSLRRADNIHWQAIDVDKERFSAHLIWMGDQKLVPGRTYAVKAGKQSATGVVSRIGHRIDVNTFAQDEVDSLSLNEIGFCNLALSAPIAFDPYEVNREMGSFILIDRMTNQTVAAGVINNTLVDAEYAGEEAAPTSRMRARIFWLHDKPLTAGASILVRLGTRETRATVVAIEKAVDPGELTSVETKSIARNHVGEIDIALAHPTAADPYTDNPRTGRLVIEVNGRIAGGGLQFQATMLWMSDSAMLPGRSYVMKMGARECQMQVTEERYRIDVNTRAHEAAKTLGLNEIGVVNIALDREVAFDPYNDNRRMGGFIVIDRMTNETVGLGL",
    "response#3": "TMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGQDIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTKRHSYLAHLIGIRNIVLAVNKMDLVDYSQAAFDKIVADYREFAHSIGIENFVAMPISGFRGDNITALSEVSDQFQATMLWMSDSAMLPGRSYVMKMGARECQMQVTEERYRIDVNTRAHEAAKTLGLNEIGVVNIALDREVAFDPYNDNRRMGGFIVIDRMTNETVGLGLFETTIVWMSDENLIPGRGYWLKLATQTISATVAEPKYEIDVNSLEHLAAKSLSLNSIGVAEVTTDKPIVFECYKDSKQLGGFILVDKITNATVAAGMVSDQFTVHMVAMSEQALMPGRPYLVQIGTRQVGAQITHIKHKINVNTLEHTAAKRLELNDVGVCNIGFDRAVPFETYADNRNMGGFIVIDRISNQTVGCGMIRQPLRTDQFETTIVWMSDENLIPGRGYWLKLATQTISATVAEPKYEIDVNSLEHLAAKSLSLNSIGVAEVTTDKPIVFECYKDSKQLGGFILVDKITNATVAAGMIRPVAIRHAHQPRAAVSTEATRFITCGSVDDGKSNLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGQDIDFALLVDGLAAEREQGITIDVAYRFFATEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLSQTRRHSFLAHLIGIRHIVLAVNKMDLVGYDQATFDEIVSDYSNFAESIGIKDFTAIPISGFKGDNI"
  },
  {
    "reference": "MIEFSNFYQLIAKNHLSHWLETLPAQVAAWQREQLHGKFKQWSNAVEFLPEMTPYKLDLLHSVTAESEEPLSDGQLLRLDKLMRNLMPWRKGPFSLYGLNIDTEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPMQLFLCQFEAVRKLLGNDQRAHLLPLGIEQLPALAAFDTVFSMGVLYHRRSPLEHLWQLKDQLVNGGELVLETLVIEGDENAVLVPGDRYAQMRNVYFIPSALALKNWLEKCGFVDVRIADVCVTSTEEQRRTEWLTTESLAEFLDPNDSTKTIEGYPAPMRAVLIATKP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must be able to regulate tRNA wobble uridine modification.2. The carboxy-S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MGWKGAQAMQFGQPLVFDMAYENYMKPRELQNAVSQLLESEGCNRRNVDPFHIYFCNLNIGSVYHRELVKRYGEKWDKLLLTATEKSYVDLFSKDSIIYLTADSPNVMTTFRHDKVYIIGSFVDKTMQPGKSLAKAKRLKLATERLPLDKYLQWSVGTKNLTLDQMMRILLCLKNTGNWEEALKFVPKRKHTY",
    "response#2": "MGWKGAQAMQFGQPLVFDMAYENYMKPRELQNAVSQLLESEGCNRRNVDPFHIYFCNLNIGSVYHRELVKRYGEKWDKLLLTATEKSYVDLFSKDSIIYLTADSPNVMTTFRHDKVYIIGSFVDKTMQPGKSLAKAKRLKLATERLPLDKYLQWSVGTKNLTLDQMMRILLCLKNTGNWEEALKFVPKRKHTC",
    "response#3": "NYMKPRELQNAVSQLLESEGCNRRNVDPFHIYFCNLNIGSVYHRELVKRYGEKWDKLLLTATEKSYVDLFSKDSIIYLTADSPNVMTTFRHDKVYIIGSFVDKTMQPGKSLAKAKRLKLATERLPLDKYLQWSVGTKNLTLDQMMRILLCLKNTGNWEEALKFVPKRR"
  },
  {
    "reference": "MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQRLEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAETASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDIDISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLSGRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFKAFGRALRMAVEHDPRMAGQTPSTKGTLTA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that can modulate the overall flux of metabolites through the L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9 pathway, enhancing or suppressing its activity as needed.2. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "PLKLSSNENPSGCSPAASAKVAETALDLHRYPSTDHSALRMAIGQVHGLDAGRIICGVGSDELLTLLAMAFAGPGDEVLYTEHGFSMYRIFALSAGATPVVAPEKDRTVDIDAIIDRLTPATRLVYIANPANPTGTAIGLEALRRLAQSVPPTSLLVLDGAYAEFFEGYDGGASLVDEFDNVVMTRTFSKLYGLGGLRVGWAYATQEVIDVLNRVRGPFNLSSVALAGAEAAVNDRAFVETCLQENAAQRVRLMGGLNQLGIACDPSHANFVLARFADEAEALAADAHLKSEGILVRIVKGYGFPEALRITVGRADDVTRVLDAL",
    "response#2": "DLAATHFPAAALDLLRQFLAIRVSLDMAAVTLRAFAADNALDLGAVLQKFEARADAIAQAGIEMKDIIYDASFGRPLDYYTGLVYEIRDASNRQDGVLAGGGRYDRLLTMLGACEAIPGVGFSIWLDRLS",
    "response#3": "DMIKLNTNENAYGPSPKVAEALQNFDDQELRKYSSLDQTELCQALAANLGVSPDQLIIGNGSDDILSMAFLAFFNSGESVLFPDLTYGFYKVWADLYHVPFREIPLTDDFEVHPSDYFGDNGGIILANPNAPTGIYLPLEQLEEILASNQDVVVVVDEAYIHFGGQSALPLLETYPNLFITRTFSKDAALAGLRVGYGLGHPDLIAVMKAVKDSINPYSVDLLAESLALAAVQDWDYYLETGRAIQETRDWFAGELAALDFQVLTSQTNFVLAQPSGISAAELFQQLEERRIYVRYFPKAERIKDFLRISIGRREEMETVLAAG"
  },
  {
    "reference": "METADVTPPQIISILGEESIIAKYGIWPNFVATDLLSQVRSSTYVLITDTNLHDTYVPSFRTFFEANKIAAGVRLLTYAIAPGEGSKSRETKAEIEDWMLSQSCTRDIVIIALGGGVIGDMIGYVAATFMRGVRFVQVPTTLLAMVDSSIGGKTAIDTPMGKNLVGSFWQPKRIYVDLAFLQTLPVREFINGMAEVIKTAAIWNEDEFIFLEQYAAEITSCIRSKSKDRLASIKEILARIVVGSAKVKAEVVSADEREGGLRNLLNFGHSIGHAIEAILTPQLLHGEAVAIGMIKEAELARYLGILRPHAVSRLSKCIASYGLPTSLQDKRVVKLTAGKRCTVDNLLKKMTVDKKNDGQQKKIVLLSKIGKTHEQKASAVADKDIETILSSSVIINPGVPSGLTVTVTPPGSKSISNRALILAALGSGPCRIKNLLHSDDTEYMLSAISQLGGASYSWHDAGEILEVEGKGGNLSASRDELYIGNAGTASRFLTTVLALCSPIRQTCSTVLTGNARMKVRPIGPLVDALRLNGVEIEYLGQENSLPLRVYSTAGFPGGVIELAATVSSQYVSSILMAAPYAKRPVTLRLVGGKPISQPYIDMTISMMKTFGVSVAKSATEADTYHIPLGTYTNPSEYIIESDASSATYPLAVAAITGTTCTIPNIGSSSLQGDARFAIDVLRPMGCSVKQDELSTTVTGPAPGHLKPLPRVDMEPMTDAFLTASVLGAVANGKTCITGIANQRVKECDRIAAMRNQLAKFGVVCTELD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a binding site that is specific to the target Zn(2+), NAD(+), 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate, ATP ligand.2. The presence of 3-dehydroquinate synthase, Shikimate dehydrogenase substrate binding N-terminal, Enolpyruvate transferase domains is critical for the interaction of the designed protein with its intended targets.3. The protein's Proton acceptor; for 3-dehydroquinate synthase activity, Proton acceptor; for 3-dehydroquinate dehydratase activity, For EPSP synthase activity must be carefully engineered to ensure that the amino acids involved in NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH are properly positioned for optimal catalysis.4. The ideal protein would be one that plays a critical role in amino acid biosynthetic process, phosphorylation, chorismate biosynthetic process, aromatic amino acid family biosynthetic process through shikimate kinase activity, shikimate 3-dehydrogenase (NADP+) activity, 3-dehydroquinate dehydratase activity, ATP binding, 3-dehydroquinate synthase activity within cytoplasm.5. The protein must bind to Zn(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MKYEVAPGEYSKSRSTKAAVEDWLLRQGCTRDTVIIALGGGVIGDMIGFVAATYMRGVRFVQVPTTLLSMVDSSIGGKTAIDTPAGKNLVGAFWQPERIYVDLQFLETLPKREVAAYEIPPGESSKCRQTKDDIEDWLLSQSPPCGRDTVMIALGGGVVGDLIGFVAATYMRGVRFVQVPTTLLAMVDSSIGGKTAIDTPLGKNLVGAIWQPQRIYIDLDFLGTLPRRELGFNVYSYPCAGRLYLGNAGTAARFLTGGMAEVVKTAAIWDEKEFERLEEGADAIMAALDKPVGKGRFAGIEDVFKRIVLGSARVKAEVVSSDEREGGLRNLLNFGHSIGHAYEAIWTPEILHGESVAVGMVKEAELARYLGVLPPGAVARLTKCIASYGLPTAIDDKLITKWTDKQCPADELLRRMAVDKKGMAEVVKTAAIWNENEFLSLEARSAEIFAAIQTPSQNFAGRTKNTRSAAQELLLSVIVGSISVKAHIVTIDERETGLRNLVNFGHSIGHAIEAVLTPTILHGECVSIGMILEAELSRQMGILSQVGVGRLSRCLKGYNLPISLSDPRIANLPAARLLTVDRLLDIMRIDKKGMAEVVKTAAIWKDDDFAALESRASEISLAASSKSTKSPTAGRFAADRSQPQSLLLQVVSGSIYVKAHIVTIDERETGLRNLVNFGHTIGHAIEAVLTPAMLHGECVSVGIVLEAEVARSLGVLSQVAVGRLTRCLEAYGLPVSINDRRITALPASSGLSVDRLLDIMRIDKK",
    "response#2": "YIIPPGEASKSRQTKAELEDWLLSRQPPCNRDTVFVALGGGVIGDMIGYVAATYMRGVRFVQVPTSLLAMVDSSIGGKTAIDVPFGKNLIGAIWQPQKIYLDMEFLETLPAREFEHPVYSQLRDEVTPPGSKSISNRALVLAALGTGTCRIKNLLHSDDTEHMLTALEQLGGITYSWEEDGDVLVVNGNGGKLLACENALYLGNAGTAARFLTTVATLISPSEKYDSVVLTGNDRMKQRPISPLVDALRANGSDIEYVEGHKGMPIRVPSTGGLKGGTIELAATISSQYVSSILLAAPYAREPVTLKLVGGKPISQLYVDMTCAMMESFGVKVVKSETEEHTYHIPQQVYKCPAEYVIESDASSATYPLAVAAITGTTCTIPNIGKKSLQGDARFAVDVLRPMGCTVEQSDYSTTVTGPPKGQLKPIPHVDMEPMTDAFLTASVLAAVCNGVEKTNITGIANQRVKECNRIAAMVHELGKFGVVAGELER",
    "response#3": "YEIPPGESSKCRQTKDDIEDWLLSQSPPCGRDTVMIALGGGVVGDLIGFVAATYMRGVRFVQVPTTLLAMVDSSIGGKTAIDTPLGKNLVGAIWQPQRIYIDLDFLGTLPRRELADAYAVKPGEANKTRSTKADIEDYLLSKGCTRDTVIVAIGGGIIGDMVGFVAATFMRGVRVIQVPTSLLAMVDSSIGGKTAVDTPLGKNFIGAFWQPQFVLVDVKWLETLPRREFSGLEVVSALYLGNAGTAARFLTRAPGMAEVIKTACIWNGQEFERLESNAELFLRVVNASKVINVSDTKVGQANSIHYTNIEAMLEHTFKLVLESIRVKAEVVSSDERESSLRNLLNFGHSVGHAYEAILTPQALHGECVSIGMIKEAELSRYLGILSPTQVSRLSKVLVAYGLPISPEEKWFKDLTLQKKTPLDVLLQKMSIDKKGMAEIIKTAAIWDVQDFEKLESESDAIRAAVMSEAARAHGSSQGHTLETRTTSQTLLLDVIRGSVGVKAWIVTVDEKETGLRNLVNFGHSIGHAIEAVLTPDMLHGECIAIGMVLEAEIARLVCGLPQVAIGRLTRCLQLYDLPVSLADPRIVALSKARELTTARLLDIMRVDKKGMAEVVKTAAIWKDDDFAALESRASEISLAASSKSTKSPTAGRFAADRSQPQSLLLQVVSGSIYVKAHIVTIDERETGLRNLVNFGHTIGHAIEAVLTPAMLHGECVSVGIVLEAEVARSLGVLSQVAVGRLTRCLEAYGLPVSINDRRITALPASSGLSVDRLLDIMRIDKK"
  },
  {
    "reference": "MADGFSLNDALSASGHPNPQGWPQGPWGNQPAGTAAYPGYPGAYPGAGAPGAYPGAGAPGAYPGAGAPGAYPGSGAPGAYPGSGAPGAYPGPTAPGAYPAPGQPSAPGAYPHSGTMGGPTAPMGGPLMVPYDLPLPGGIMPRMLITIIGTTKPNANKQLGLSDLPRVTQLVNVKLTLDFKKGNDVAFHFNPRFNEGGRRVIVCNSKFSNNWGTEERHATFPFEAGKPFKIQVLIEADHFKVAVNDAHLLQYNHRMKNFHEINKLGISGDVNLTNTSYTMI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that can perform protein phosphatase binding, laminin binding, molecular condensate scaffold activity, IgE binding, chemoattractant activity in mitochondrial inner membrane, nucleoplasm, cytosol, spliceosomal complex, immunological synapse to support positive regulation of protein-containing complex assembly, positive regulation of protein localization to plasma membrane, eosinophil chemotaxis, macrophage chemotaxis, epithelial cell differentiation.2. The designed protein should contain one or more Galectin domains that are essential for its function.3. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "FKTAFANGVMMYSRGTQGDYYALQLKDNKMVLNLDLGSRVMTSLSVGSLLDDNVWHDVVISRNQRDIIFSVDRVIVRGRIQGEFTRLNLNRELYLGGVPNVQEGLIVQQNFSGCLENIYFNC",
    "response#2": "FRTYEETGVMLHHDFYSGGYLKVFLEFGKVKIDLKVKDKARIILDNYDDQFNDGKWHSFVISIEKNRLILNIDQRPMTTTKC",
    "response#3": "LAWQCLTREIQLIRSWSLVTFRTLKPEEQRQALRNLEMHYQAFLRDSQDAGGFGPEDRLQVERDYSTCTRHYQQLLQA"
  },
  {
    "reference": "MAALDQQPLLHDGGDQKPPPEGAARRFRRCRTAPSSEPPPTDKDNSSAADAPPKTLFTGGGRPSFRLVGLLLVAYLLLGTIAFYLAMDHMSGTRTTRALDALYFCVVTMTTVGYGDLVPASDAAKLLACAFVFAGVAVVGTFLSKAADYLVEKQEALLFRALHSHTMVRAMEMNKVRYKLYTAGLLLVAAVASGTVVLWKVEGMRAVDAFYCVCATVTTLGYGDRSFSSEGGRAFAVAWITVSTVVVALFFLYAAELYTERRQRELARWVLRRRTTNMDLEAADLDGDHRVGAADFVLYKLKELGKISQEDISEFLDEFDNLDADHSGTLSPADLAAAQPTPDPPPSLR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The designed protein should have a binding site that is specific to the target Ca(2+) ligand.3. The designed protein should contain one or more EF-hand domains that are essential for its function.4. For general function, the protein need meet that Functions independently of the voltage difference across the membrane.; Highly selective inward-rectifying potassium channel that is specifically located in the tonoplast of protein storage vacuoles5. The designed protein should have metal ion binding, outward rectifier potassium channel activity, potassium ion leak channel activity to facilitate stabilization of membrane potential. The designed protein sequence is ",
    "response#1": "VLFSSRMVISDFEGKPTLMMRLANLRIEQIIEADVHLVLVRSEISQEGMVFRRFHDLTLTRSRSPIFSLSWTVMHPIDHHSPIYGETDETLRNSHSEFLVLFTGHHEAFAQNVHARHAYSCDEIIWGGHFVDVFTTLPDGRRALDLGKFHEIF",
    "response#2": "VLFSSRMVISDFEGKPTLMMRLANLRIEQIIEADVHLVLVRSEISQEGMVFRRFHDLTLTRSRSPIFSLSWTVMHPIDHHSPIYGETDETLRNSHSEFLVLFTGHHEAFAQNVHARHAYSCDEIIWGGHFVDVFTTLPDGRRALDLGKFHEIF",
    "response#3": "VLFSSRMVISDFEGKPTLMMRLANLRIEQIIEADVHLVLVRSEISQEGMVFRRFHDLTLTRSRSPIFSLSWTVMHPIDHHSPIYGETDETLRNSHSEFLVLFTGHHEAFAQNVHARHAYSCDEIIWGGHFVDVFTTLPDGRRALDLGKFHEIR"
  },
  {
    "reference": "MSIAPAAHTPMMQQYLGIKAQHPDMLLFYRMGDFYELFFEDAEKAARLLNITLTTRGASAGSPIKMAGVPYHSAEQYLARLLKLGESVVIAEQVGDPAASKGPVERRVSRVVTPGTLTDAGLLDETRDALIMAIAVAGDVLGVAWLNLAAGRFQVTELDRTALPALLARVRPAEILAHEHLDLAADCPVRRLDAWQFDADGASKRLARQFGSCDLQGFGVAEMHCAIAAAGALLGYIETTQRTALPHLLSIRAERDSDFVLLDAATRRNLELTETLRGDAAPTLRSVLDTTCSGMGTRLLRHWLHHPLRDRAAVAARRDAIGVLAAAPDSAARLADLLKRCADVERIGGRIALKNARPRDLSGLRDTLALLPELAAALPTDGARLAALRDAVAATPDVHALLVRAIQPEPASVLREGGVIADGYDAELDELRALTRDAGAFLLELETRERARSGIATLKVEYNKVHGFYIEVGRAQAERVPDDYRRRQTLKNVERYLTPELKAFEDKALSAQERALAREKALFEALLDTLIPHVPNLLSIASALAEIDVLASQAERASTLRLCAPEFSDDPCIVIRGGRHPVVEAQVEHFIPNDVVLNRTRQMLLITGPNMGGKSTYMRQVALITLMACCGLWVPAASARIGAIDQIFTRIGASDDLAGGRSTFMVEMTETANILHSATADSLVLLDEIGRGTSTFDGLALAWAVARHLVSATRAFTLFATHYFELTQLAQEYRQLANVHLDAKEHGADLVFLHAVEDGPASQSYGIQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be optimized for ATP binding, damaged DNA binding, ATP-dependent DNA damage sensor activity, mismatched DNA binding activity in order to improve mismatch repair.2. The protein should have a high affinity for ATP.3. The protein must exhibit the following characteristics: This protein is involved in the repair of mismatches in DNA; This protein has a weak ATPase activity.; It is possible that it carries out the mismatch recognition step. The designed protein sequence is ",
    "response#1": "MITGPNMAGKSSYLRQTGLIVLLAQAGCFVPAEQAEIGVVDRIFTRVGASDNLASGESTFLVEMNEAADILNNATAKSLLLLDEIGRGTSTYDGLAIAWSMCEYIHRQIGARTLFATHYHELAELEGLLPGVVNYNASVLESGDRL",
    "response#2": "MIITGPNMAGKSALLRQTALIVLMAQAGSFVPATHATIGTVDKVFTRVGASDNLSKGESTFMVEMNETASILNNLSGRSLVLMDEIGRGTSTYDGISIAWAIVEHLHNHPNFRPKTLFATHYHELNQLTEDLKRVKNFNVSVKEAGNKVIFMRTLKPGGSEHSFGC",
    "response#3": "LVLGESFVQEAFFIDKQQGDLRVLGFLGSPNQHRSTRQGQRLFINNRAVESSFISKKVAEAYAWMIPAQRYPIFVLKLFLPPMWCDFNVHPQKTEVRLLQEGQISNLLVEAISEAG"
  },
  {
    "reference": "MPEKYYQVGKIVNTHGIRGEVRVIATTDFTEERFKKGTHLAVEMPNGLTPVTVSAMRQHKQFYLLQFEGLGNINDVELFKGHNLKIAASERDDQLEDDEYYYGDIIGLEVIEESDGTSYGKVSEIIDPGPNDVWVIKRRGRSDLLLPFLKSVIKKIDIEAGKAYVEVPEGLIDNED",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be able to regulate rRNA processing.2. For general function, the protein need meet that It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.; Probably interacts with S19 The designed protein sequence is ",
    "response#1": "WSDLVSLRVMNEQDEYLGDITEMFETGAHAIMRVAATTDSLDKEERLIPWHKQTVVKVDLTEKTVLVAWPSI",
    "response#2": "MGPIGAKVTLRGERMYDFLDKLVNVSLPRVRDFHGVSAKSFDGRGNYTLGVREQLIFPEIDYDKVDRVRGLDVVIVTTSNTDEEARELLTQFGMPY",
    "response#3": "MERGVK"
  },
  {
    "reference": "MTRLLGYVDPSEPHFVAAVLTIAFNPLFWNVVARWEPKTREPSGAFGSPAPACCTLGGTILLLNVLRSTQAMLSQSLDNPSAYRVGLALLGVGGVFVLSSFLALGFTGTCLGDYFGILKEARVTTFPFSVLDNPMYWGSTADYLGWAIM",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be able to bind S-adenosyl-L-methionine ligand in a variety of conditions2. The designed protein should contain Lumenal topological domains.3. A protein that contributes to methylation, phosphatidylcholine biosynthetic process by functioning in endoplasmic reticulum membrane with phosphatidyl-N-dimethylethanolamine N-methyltransferase activity, phosphatidylethanolamine N-methyltransferase activity, phosphatidyl-N-methylethanolamine N-methyltransferase activity. The designed protein sequence is ",
    "response#1": "MCKYTWL",
    "response#2": "LMFGKR",
    "response#3": "AVTIRKL"
  },
  {
    "reference": "MEEKEGIKDSSLLEKSNVPESINEDISKTTDVDLNSDGKKDNDTSAKDGTPKVEEKVNKSSGIDEDEVVTPAEDAKEEEEEHPPLPARRKSEEEPSKENPILQELKDAFPNLEEKYIKAVIIASQGVLSPAFNALLFLSDPESGKDIELPTQPVRKNPEAPARRRQTQLEQDELLARQLDEQFNSSHSRRRNRDRATRSMHEQRRRRHNPNEREQHHEDSEEEDSWSQFVEKDLPELTDRAGRSLQDTANKVSNWISDAYRRNFASGNEQNDNQHGHQDQQEWEPEIVDLSQGGKNSRPQQPERRRFNSFGVQVGDDSLESHGITLHNEDGFEDDEDVPPQLPTRTKSGESTGKVVAETTYIDTPDTETKKKWQPLPPEPLDTTPTKVNAVSRNKKNPDEDEFLINSDDEM",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The ideal protein would be one that plays a critical role in ubiquitin-dependent protein catabolic process, autophagy through protein-macromolecule adaptor activity, ubiquitin conjugating enzyme binding, ubiquitin binding within cytoplasm.2. The presence of CUE domains is critical for the interaction of the designed protein with its intended targets.3. Prioritize Basic and acidic residues, Polar residues compositional bias within the protein sequence to achieve desired characteristics.4. The protein design must feature the AIM motif.5. For general function, the protein need meet that Not required for cytoplasmic to vacuole pathway (cvt), mitophagy, pexophagy, or ribophagy.; The CUE5-dependent selective autophagy pathway plays an important role in clearance of cytotoxic protein aggregates; Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 adapter and thus mediating autophagic clearance of ubiquitin conjugates under starvation conditions The designed protein sequence is ",
    "response#1": "IQNFHTKAAIIVLGARVGLPVVFTKEGTKKVNKWFQIETDDTDAYRDELHTWRMCGGFQNRPPPLIIETYLDTSHLTSSQRLVIVDDLGKRWDALDALSNSCGSNDGRSEVILERWKIELRDIPGEQPYDFGSTLPTIYKKCIVFFRSLYSTAKLVPAWKFKKSLGKNGSPTNSLIINCRITTGDTQPRRIDGLKQPILDHSGPVTSTYVLGATDTPAGQIFAEVTYRNDCNWRISPQLNLEKVAQQKVLLLGSGTLGSYVARALMGWGVRHITFVDNGRVSYSNPVRQPLFGFNDCFSDEGLGRWKAPRAAEALKEIFPGVNSSAYNLEVPMIGHPVENEQSAKANYETLERLVDENDAIFLLMDSRESRWLPTLLGMAKNKLVINAALGFDSFLVMRHGTTTNNLGCYYCNDVVAPSDSLSDRTLDQMCTVTRPGGALMASALAVELLVSVLQHPEGAEADACGTCGDLKYVPTRSFVESSFFKALSDLKLNQLKLNTDTVSISGFMNHPKNLTKFQDYPILNLDFSSFELASSASSAASDENVEYKGTLLNVNTIEEFKAINKSDLLKQWGSLIYKSITEGDTTYRSVNQFFVLTFSDLKKYKFYYWFAYPSLQSQWTVKECKEAPSFEDETRKYLDTQEELEFFFAREQEEFCGLSKFLNGSKKVNEHSTFVFVDSCRHPGLYPGWQLKNFLYILARQGMREIRLFVYRYDGTSIMMKLQLDSIPNDIKFMGWERTKDDKLGPKLADLGS",
    "response#2": "PMCDECAELLIENYKLKFDQNQREKDSYMTFLKKLKLKDDSDIKESDLDTKLRDSIQECRDLAASEQEKLQELRSLEQNREELSKELQDVKMELALQQSTELSNALRLKNELHWTLQRKADQLAQEKARYRVVLNRAVEASRAQRSLSSINHVWRANEIVTAARAALEECVVVSARTAFLRRGLNNQLRLLYSVRTEVEEISLRGRSEFAAVLKDLDDADTRLRRTLELLRETIVHPAFRPEGEDPKSLHDFVDERGVEELHAALKSSIDRTTAAQAQLDSSNHAFDDELLSIKEALGTYRTAAKLASSRSSSSASSSSTSNSSLPSISTMPSMLHSLEMHAQEMANLLESLVRHFDLCVTAVKHTEGGGAAAKSITGDMPVGVPVSGRMGSNIEEEINNNLNAPLDPLSNSEYQEMVNVLFKDAAEAEDVVMEIQDRIGEMESVLENVLAQRDALRSIYNATTDIYQHLSSLGSTRLPGYIAQAHNFTQVWHEENDRISGGLADLSDLNSLYNGFLDAYDGLIVEVARRKHVRQRVEKVLRDTRHKLDQLYEEDVTARETFRVEKGDYESRGLC",
    "response#3": "TLTNQNDLQAWRNLYMARRSWALGLVERCGAMDKSIHEHNERTDIIHRAAGVALENLKTHVGNLENRFQEAQTWANDLLKEQRAALDGWQRALTTLESIPAPKVFPFLGRPSTPKEHRDRPTGTLRDFVDANEVQKAGAEAAAESSRFARQIDDVAEAVRGITADTQRLIDDQLPSGADAADGLQEMITFAKKISSDYEHVIALPNNQKTLANISRLALTHTQDLLPSMLDISAEIHAGLEEAVRRHNTAMKVALDHMRTISAIELRLADVQSQIINLNVQSDAFDVVFSVYHMPMVYGSILVESVRRREFNEKMKADSLTLAEEMAVFRDEEQRRRKKWLKSMGDFL"
  },
  {
    "reference": "MAMNFGDHASGFRHNDVIRFINNEVLMDGSGPAFYVAFRSRPWNEVEDSLQAIVADSQVPRAIKRACTWSALALSVRVATRQREELLHHVRRLQRHAEERQATSWALTSQLQQLRLEHEVAATQLHLAQAALQQALNERDGLYGRLLQIERFPQAAPLAHEIMSGPQAEQNGAAACPLATEQQSDMVAMGTHANAQMPTPTDVLYVPGPLSPWAQGMQPPLPVPHPFPHPPPFPMKFPSLPPLPPAVVTGAEAAAVPLQMPPTEIHPPCPWPAVGFQEEMAPLWYQRSYIQEEDSKILQGSFPLGDSRSHSQGEGSERSQRMPLPGDSGCHNPLSESPQGTAPLGSSGCHSQEEGTEGPQGMDPLGNRERQNQKEGPKRARRMHTLVFRRSHKSEGPEGPQGTVPQGDSRSYSQEGCSDRAQEMATLVFIRRCKPEGPKRPQWTVPLGDSRSHIKEEGPEGPQRIVLQGDNRSYSQEGSPERAQGMATLVFSRSCKPEEGPERPQDTPLGDSRSHIKEEGPEGPQRIVLQGDNRSYSQEGSPERAQGMATLVFSRSCKPEEGPERPQDTPLGDSRSHIKEEGPEGPQRIVLQGDNRSYSQEGSRERAQGMATLVFSRSCKPEEGPERPQGTPLGDSRSHGVRESPKKWQPQRQKAKKPKVNKVSGSQQQEKPASFPVPVNWKCPWCKAINFSWRTACYKCKKACVPFESGGQTQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein can be expressed in cytoplasm and perform RNA binding, mRNA binding without triggering undesirable cellular responses.2. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "ETLAELRNNNKTQINLLTILADDFKKAAPQIVEVIERHLTTCSPSQKLLVMYVCDSILKNVKKPNDYDALFARKIVSMFEHAFRQGDERIRTSLYRIRVTWASTTLFMPSKLYA",
    "response#2": "ARDYRETLAELRNNNKTQINLLTILADDFKKAAPQIVEVIERHLTTCSPSQKLLVMYVCDSILKNVKKPNDYDALFARKIVSMFEHAFRQGDERIRTSLYRIRVTWASTTLFMPSKLYELDMKINKLDPNR",
    "response#3": "MEVEVFTRTNDRETCGWWVGIIKMRKAEIYAVAYIGFETSYTEICELGRLRAQ"
  },
  {
    "reference": "MSLSGKNILLGVTGGIAAYKSAELTSKLVKSGAEVHVVMTESAKKFIGPATFQALTRHPVYDSVFIEEKDGQIAHIDLADSADLFIVVPATANTIGKLASGLADDMLTAAALASRCPVWIAPAMNVNMYTHPAVQKNLRTLESFGYRLIGPDAGWLACGWTGKGRMTEPDEIFNTIDRYFSAEKKFTLPLAGKKILVTAGPTREALDPIRFFSNQSSGKMGYAIAEAAGEAGADVTLISGPSALSDPSGISVIRVTSADEMRREVLARYEHADAVIKAAAVADYRPESVSGSKIKKADGPLLVKMVRNPDILMELGRIKKSQLLVGFAAETEQLEDNARAKLEKKHLDLLIANHAADGFAKDTNKVTFFFADGHQKAYGEMPKKEIAEKICRTVSELMETSGQR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..2. The presence of Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.3. A protein should be able to manipulate the activity of enzymes in the Cofactor biosynthesis pathway, either by activating or inhibiting their function.4. The protein design must be able to perform FMN binding in order to contribute to coenzyme A biosynthetic process, pantothenate catabolic process.5. The designed protein should have a binding site that is specific to the target CTP ligand.6. The Proton donor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "KKILLGVSGSIAAYKTALLIRLLVKEGAEVKVVMSSSAQTFITPLTLATLSKNPVLTDFFNPHDGTWNNHVELGLWADLFIIVPATANTMAKLANGICDNLLGTTYLSARCPVWFAPAMDLDMWLHPATRDAVAKLGSFGNRILEPGTGDLASGLEGKGRMMEPEEIFASLLERVETFLRPRPLSGSRLLITAGPTREAIDPVRYISNHSSGRMGYAVAMAGIEAGAEVSLISGPVNLSAPSRVELINVVSTAEMYSAVMQKVGSTDIFISAAAVADFSLQTIAYKKIKKSSGAFELSLQKNPDILAEVAALNSPPFTVGFAAETENIELNAQAKLRSKRLDMIAANQVGQGLGFDSEMNTLTLFWPGGQKALPLAPKSLLATKLLNEIASLLP",
    "response#2": "KSIILGITGGIAAYKGAALCSKLVQAGANVRVIMTESATKFITPLTLQTLSRNPVYIDTFDERDPAVVSHIDLADHADLIVIAPATANAIAKLAGGHADDMLSTTLLAATCPVLIAPAMNVHMYEHPAVVSNLELLASRGAMFVEPGTGQLACGYVAKGRLAEPEDIAAAIEAVLAPKALPLSGSRLLITAGPTREAIDPVRYISNHSSGRMGYAVAMAGIEAGAEVSLISGPVNLSAPSRVELINVVSTAEMYSAVMQKVGSTDIFISAAAVADFSLQTIAYKKIKKSSGAFELSLQKNPDILAEVAALNSPPFTVGFAAETENIELNAQAKLRSKRLDMIAANQVGQGLGFDSEMNTLTVFWPGGGKELPRASKETLARQLVQEIARRYNAARDTE",
    "response#3": "MSGTRVMVTLGPTREKWDGVRYWTNPSSGVMGASLAMAAWLRGARVDAVCGPGAPWLPSDIVRHDVGSADEMFEVAHDLWPDVDTGIFTAAVADFKPVPYGAEKFKKADAADGFDIRFAPNRDIIATLGALRRPGQRVVGFAAETSNLIEAMRGKLKRKNADMIVGNLVGSSDAGFGAATNRVRSPREIAEAALAVLEPRPLTGRHVLITSGPTHEPLDPVRYIANRSSGKQGHALARAALALGARVTLVSGPVNLPNIRGVNAVKVETAQEMLDAVLDALPADIALCAAAVADWRASAEAAQKLKKERGADVSLSLAQNPDILATIAIPGPQRPELVIGFAAETRNVVDHARAKRRAKGADWIVANDVSAATGIMGGDRTQVHLFDARGSVSLPAATKREIARRLIAEVARRYAAGG"
  },
  {
    "reference": "MPAAAAPLEKTPLEIYVPPAQPSLVGLSRAELADCLGEVGVPPAQRRMRVQQLWHWIYVRGAQSFDAMSNVSKELRARLAERYTLARPEVVVEQVSSDGTRKWLLRLPGEHPGERPHEVECVYIPETDRGTLCVSSQVGCTLTCAFCHTGTQRLVRNLTPGEIVGQVMIARDRLGDWNDRDTPSGGRLVTNIVMMGMGEPLYNFEAVRDALLIVADNEGIGISKRRITLSTSGVVPNIARTGAEIGVSLAISLHAVRDELRDVLVPINRKYPIAELLEACRTYPGVSNARRITFEYVMLKGVNDSLDDARQLVKLLKGIPAKINLIPFNPWPGTAYECSDWAQIEKFSEYVFNAGYSSPVRTPRGRDILAACGQLKSETERLSARERQALRAMAMTD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must be able to rRNA (adenine-C2-)-methyltransferase activity.2. The protein should have a high affinity for S-adenosyl-L-methionine.3. For general function, the protein need meet that m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs4. The desired protein should be able to perform adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine The designed protein sequence is ",
    "response#1": "DADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNLTPGEIVGQVMLARDELGEWPKGRMDIAAEESEAEYRADGRLLTNIVMMGMGEPLYNFDNVRDALKLVMDNEGLGLSRRRITLSTSGVVPAIARCGEEIGVNLAVSLHAVRKEVRDEIVPINRKFGIEELLEACAAYPGASNARRITFEYVMLKDKNDSDADAHELVRLIKKYDLPAKVNLIPFNPWAGAPYECSTPERIRSFSNIVFGAGISAPVRTPRGREPSRGTLCISSQVGCAMDCSFCATAQQGFNRNLTAAEIVGQVLIAKRMLEPGPDGRRAITNVVLMGMGEPLANYRNVVPALKILIDDFGFELSRRRVTLSTSGIVPWIDKLADDCNVALAVSLHAPHDALRNELVPINRRYPIAKLLDACWRYASAYSSRQVTFEYVMLDGVNDGREHADRLIRLLRNRPAKVNLIPFNPFPGTHYRRSPDDVIDRFRERLLGGGVLATTRRTRGDDLAAACGQLAGGVV",
    "response#2": "MNDRYSQCLSTQVGCAMACTFCNTGKLGFERNLTYGEIMGQILVGRQYLEDQGLNPLKNLVFMGMGEPLLNLDTLIKVLTDLPCERGLSLSWRRSMVSTVGFPDKLKILGDLEIALPAISLHAPTQELRAKIMPKAAKVHLDDLMASLKVYPMRSRERITFEYLLLKGVNDSMEHADQLARLIDRRKGKINLIAYNATEGMPYDAPDRAQVEAFEKRLWDHGMTAFIRRSMGSREHMQEQGLRSLAEHESLMAGW",
    "response#3": "MNDRYSQCLSTQVGCAMACTFCNTGKLGFERNLTYGEIMGQILVGRQYLEDQGLNPLKNLVFMGMGEPLLNLDTLIKVLTDLPCERGLSLSWRRSMVSTVGFPDKLKILGDLEIALPAISLHAPTQELRAKIMPKAAKVHLDDLMASLKVYPMRSRERITFEYLLLKGVNDSMEHADQLARLIDRRKGKINLIAYNATEGMPYDAPDRAQVEAFEKRLWDHGMTAFIRRSMGSEARLGERIVREALGA"
  },
  {
    "reference": "MDWPKPDSDHLIVTSAEMECLEKLLISQGMPVESLMEKVGLGMRNWILKRSDILKNGVVFLVGPGHNGGDGLVLARELFLLGFQVSLWCPFLLKKPLTIKHFEYCKWLGIQILERPPEASCDSLWIEALFGIGQDRSLPDEISTLLKARDESQPGRLISLDVPAGICSDTGKSLAKSAAKASFTLTVGLVKQGLIQDLALPYVGKLVRVDFGIPQIVLDQKHSKNHLRVMSDDLKHISWPHPSKELDKYHRGRVLVLAGSDQYKGAASLALKGALASGVGSTSAFLDNSIANDLWQVLPEVVVSCAFRNLDDCFTNLKKTFASSNLNRFDSVLIGPGFLPAKNSWTEFGQILEDFSGLLVVDADALNGISLSEEGWKWIRRREGPTWITPHPQEFSRLFPDLKNLVPLEAASKAAIRTSAGVLLKGAHSVIASPSGSVWQLGQTSSLVARTGLGDVLAGFLAGIGAVGVALKPKCLNCDLLAAAAFLHAEVASNYTEGSNASSICTSLEKITRRIQERKCIQRYI",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.2. The protein must be able to ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding, metal ion binding. The designed protein sequence is ",
    "response#1": "VMVIGGSSEFVGAPWLAALSSLYGGVDLVYLAAPAPVLSSIISPEIIPIKLHGERLVEAHVERLVSEIVSRRVHVLVLGPGAGRCEETINFFCKLYEKLTHLEHVSLLGIVVDADALYSSAQCLGHLQEVGRRYNVILTPHLGELKRLLGREIADTLEQRIEASKEAAKKTNSVVLLKGAIDVIASPNGKIKLNRTGTAQMTVGGTGDVLAGLVAAFLARGANPFYAAALAAFVNGLAGERVVKNVGRVTPMLLIEWLR",
    "response#2": "AGPMPGAAALAASAVLRAGGGYAILSGATSVPFSAIIAERGENFAERLLDERLGAVVIGPGYPPGAALDRDVAAALKSGKPLVLDAAAIPSALPRLGDTPAILTPHAGEFARAFPGLEGSKIEQARAAARRSGAVLVYKGADTIIAAPDGRIVAAWPGCPWLATAGTGDVLAGACGAMLARGGDAFDAAVAAVSWHISRAR",
    "response#3": "PDSHKGTFGHLLVVAGSEGMTGAAALAGEAAMRVGSGLVSVAVPQSLRTVIEMKLTECLKLGLEESEPGVLGEGAVRQLTPHLHRYHATLLGPGLSTRGEVARVVVALIERAEHPMVLDADALNILAGTLESLEVAKAPLILTPHPGEMARLLRTSTARVQENRLSIAHDFATRHGVVLVLKGARTLIATPEGEVFVNPTGNAGMASGGTGDVLAGMIGGFLVQGHPPRTAALLGVYLHGLAGDLAAEALGERSFIASDLLDRLAA"
  },
  {
    "reference": "MGGKWSKSSIVGWPTVRERMRRARAEPAADGVGAVSRDLEKHGAITNSNTAANNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRYPLTFGWCFKLVPVEPEKVEEANEGENNCLLHPMSLHGMDDPEKEVLQWKFDSRLAFHHMARELHPEYYKDC",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should contain at least one Diacidic; necessary for CD4 internalization, Dileucine internalization motif; necessary for CD4 internalization, PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif to achieve its intended function.2. The designed protein should have SH3 domain binding, GTP binding within extracellular region, host cell Golgi membrane, membrane, host cell plasma membrane, virion component to regulate suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host autophagy.3. For general function, the protein need meet that Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPAVRERIRRTEPAAEGVGAASQDLDKYGALTSSNTPTTNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLVYSQKRQDILDLWVYNTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPEEVEKATEGENNCLLHPMNQHGIEDPEREVLQWKFDSRLAFHHVARELHPEYYKNC",
    "response#2": "MGGKWSKCSGWPAVRERMRQAEPAAEGVGAASRDLEKHGAITSSNINNADSVWLKAQEDEEEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLDGLIYSKKRQEILDLWVYNTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVDPREVEEANKGENNCLLHPMSQHGMEDEDKEVLMWKFDSSLARRHLARELHPEYYKDC",
    "response#3": "MGGKWSKSSLVGWPAIRERMKRAEPAAEGVGAVSRDLERHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQQRQDILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVEPEKVEEANEGENNRLLHPMSLHGMEDPEKEVLQWKFDSRLAFHHVAREKHPEFYQD"
  },
  {
    "reference": "MPGGLLLGEEAPDFEANTTIGRIRFHDFLGNSWGMLFSHPKDFTPVCTTELGRAAKLAPEFAKRNVKLIALSVDSVEDHLAWSKDINAYNGATPKEKLPFPIIDDKDRDISILFCMLDPVEKDANSMPLTARGVFIFGPDKKLKMSLLYPNSTGRNFDEILRVIDSLQLTETKPVATPVDWKKGESVMVLPDLPEEEAKRCFPKGISTTKLPSGKNYLRYTPQP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein contains novel Thioredoxin domains that confer a unique function or activity.2. A protein that contributes to cell redox homeostasis, positive regulation of mRNA splicing, via spliceosome, cellular oxidant detoxification, response to oxidative stress by functioning in mitochondrion, perinuclear region of cytoplasm, cytoplasm, nucleus with identical protein binding, phospholipase A2 activity, peroxiredoxin activity. The designed protein sequence is ",
    "response#1": "VQKQAPTFKKTAVVDGVFDEVSLDKYKGKYVVLAFIPLAFTFVCPTEIIAFSEAAKKFEEQGAQVLFASTDSEYSLLAWTNIPRKEGGLGPINIPLLADTNHSLSRDYGVLIEEEGVALRGLFIIDPKGVIRHCG",
    "response#2": "MLKRCNFKNQVKYFQSDFEQLIRQHPEKIIILDFYATWCGPCKAIAPLYKELATTHKGIIFCKVDVDEAEDLCSKYDVKMMPTFIFTKNGDAIEALEGCVEDELRQKVLEHVSN",
    "response#3": "MLKRCNFKNQVKYFQSDFEQLIRQHPEKIIILDFYATWCGPCKAIAPLYKELATTHKGIIFCKVDVDEAEDLCSKYDVKMMPTFIFTKNGDAIEALEGCVEDELRQKVLEHVSS"
  },
  {
    "reference": "MGYTVAIIGATGAVGTQMIKMLEESTLPIDQVRYLSSSRSAGKLLAFKGQEVTVELTTEDSFEGVDIALFSAGGSTSAKFAPYAVKAGAVVVDNTSHFRQHPDVPLVVPEVNAHALENHNGIVSCPNCSTIQMMIALEPIRQKWGLSRIIVSTYQAVSGAGQSAINETQDQLKQVLNEGINPKDVTANILPSGGDKKHYPIAFNALPQIDVFTDNDYTYEEMKMTKETKKIMEDDNIAVSATCVRIPVLSAHSESVYIETKEVAPIAAVKEAIVAFSGAVLEDDPSQQIYPQAVNAVGSRDTFVGRIRKDLDQENGIHMWVVSDNLLKGAAWNSVQIAETLHEKGLVRPTTALKFELN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein is the optimization of the Acyl-thioester intermediate to achieve high catalytic efficiency for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.2. The protein should be able to bind NADP(+) ligand in a variety of conditions3. The protein design should be able to enhance the efficiency of isoleucine biosynthetic process.4. The designed protein have Semialdehyde dehydrogenase NAD-binding domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MLLHHDLVEWISPMTYQAASGAGAQHMRELIQQMGFLHSAADRLLDDPASAILEIDRAVSEALRSENYPREHFGVPLAGSLIPWIDRAMDSGQSREEWKAGAETNKILARQTNPIPIDGVCVRTAAMRSHCQALTIKLRQDVPINEIEEILAQANDWVRVVPNEPEASKRELTPVAVTGTLTVTVGRLRKLAMGPHYLAAFTAGDQLLR",
    "response#2": "LKPLHDVAGLQRLIVSSYQAVSGSGVAGVDELANGVAAAGDKSRELAYDGEAVAFPEPVKYVDTIAYNVLPLAGSIVDDGLNETDEEQKLRHESRKILDLPDLRVSGICVRVPVFTGHSLAVNAEFEQAMTPERARELLADAPGVELREVPTPLYAAGKDPSYVGRLRQDPGVDGDRGLALFISNDNLRGGDPTFNLKPIHDLSKIKRVHVATYQAASGAGAAAMRELEIQLKQVLNGEQPTIEKFKYQLAFNLIPHIDVFLENDYTKEEMKMHNETRKIMHAGIDVSATTVRVPVLRAHSEAVWVETEKELTVEEVRTALMKADGVVVEDDPKNYRYPMPLFVAGKDPVYVGRIRKDITNPKGITFFCVGDQGPATTLAPIHRAVGIERINVATYQSVSGAGRSGGEELGRQTAALLNFQDPEPSKFPVQIAFNLIPHIDDFQDNGYTKEEMKMVWETRKILEDASIQVNPTAVRVPVFFGHAEAVHIETRERISADAARRLLQAAPGVVVVDERKPGGYPTPAGHAAGKDPVFVGRIREDISHPRGLDLWVVADNIRGGDPTFNLKPIHDLSKIKRVHVATYQAASGAGAAAMRELEIQLKQVLNGEQPTIEKFKYQLAFNLIPHIDVFLENDYTKEEMKMHNETRKIMHAGIDVSATTVRVPVLRAHSEAVWVETEKELTVEEVRTALMKADGVVVEDDPKNYRYPMPLFVAGKDPVYVGRIRKDITNPKGITFFCVGDQ",
    "response#3": "MNVVILGATGAVGIEFRKILLERKFPIDKIRFLGASTVGTQIEFGGRTVTVEAVGNDSFKGYDIGLFSAGASISKQVARRAAESGCVVIDNSSAWRMDPDVPLVVPEVNPDDLAPIHRAVGIERINVATYQSVSGAGRSGGEELGRQTAALLNFQDPEPSKFPVQIAFNLIPHIDDFQDNGYTKEEMKMVWETRKILEDASIQVNPTAVRVPVFFGHAEAVHIETRERISADAARRLLQAAPGVVVVDERKPGGYPTPAGHAAGKDPVFVGRIREDISHPRGLDLWVVADNIRPIHQRYGVRRMIVSTYQATSGAGQKGIEELQEQTRQELAGESYTPAAFAHPIAFNVIPHIDAFQDNGYTKEEMKVVWETRKIFGDESLQVSCTAVRIPTFRTHAEAITLELERPATPQEVRELLSGAAGVEVRDDVTEKVYPMPLTATGKYDVEVGRIRQSLVFDGGIELFVAGDQLLPRLPQSLKPIHDLSKIKRVHVATYQAASGAGAAAMRELEIQLKQVLNGEQPTIEKFKYQLAFNLIPHIDVFLENDYTKEEMKMHNETRKIMHAGIDVSATTVRVPVLRAHSEAVWVETEKELTVEEVRTALMKADGVVVEDDPKNYRYPMPLFVAGKDPVYVGRIRKDITNPKGITFFCVGDQVVKLV"
  },
  {
    "reference": "MNKEALKRLIAVAAGREEPDLVIKNAKVVDVFNARVIQGDIAIVDGLIAGVGDYSCKNELDAEGQYAAPGFIDSHIHIESSYVSPEELGRLLVPHGTTTIIADPHEIANVCGLKGLDYMIEAAKRTALDVQMMLPSCVPATPFEHSGANIDAREMEKPITYDEVLGLGEFMDFPGVINGVDATIEKLLVAKRAGKPIDGHSPGVSGNALNAYASARIGTDHECATVEEMHERIARGMYVLLRQGSACYNLRTLLKGVTPVNSRRCLFCADDCQPKTILSLGHLDNHLRICAEEGIDPIMAIQMATINAAECFGLKDRGAIAPGLKADIVLMDSLTNCRVEKVWIDGVLIADSGKYLPEIKRHDISSTKGNFKVKDFSVKKLKLAIQSPQAHVINILPGGVVTSKEVVAINRNSDNEFVYGHGQDVVKIAVVERHQNTGNVAVALLQGYGIKRGAIALSVAHDSHNIIVVGVDDTDMACAVEALIAQDGGIVLVNGEEVVESMPMPIAGLMSDQCGEWVEAKLTSIHSKAHEVLGVNADVEPVMTLCFMSLAVIPEIKLTDMGLFDVTKFDFISLEA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit adenine deaminase activity. The designed protein sequence is ",
    "response#1": "IIAPGLVDPHIHIESSMVTACAYAEAALLNGTTTIFCDSHEIGNVMDVAGVEAMLEDARQAPLSIFLTVPSTVPATSAALETAGGDLTPDKIADLFDRWPEAVALGEKMDFVPVCMGDERSHAILAAALQRGRPVSGHVYGREFVAAYAASGVTDTHEAIDRDIADDLLDAGVWIFLRGGPPTTPWHSLPQAIRTITELGASHKRTAVCTDDRDADDLMLFGLDWVVREAVKAGMSPEQAWSMGSLHGATRFAMDGEIGGLGGGRRADLVLLDDGLKPQSTWYC",
    "response#2": "IITEHLTAEIEPVDGDKRPDPARDLARIAVIERHGKTGGRATGFVRGFGMARGAIASTVCHDHHNLAVVGIDYADMALAANRLRALEGGFAVAAGGEILAELALPVGGLMSLRPFEEVRDALVTLREAARSLGVTLEEPFLQLAFLALPVIPHLKITDRGMVDVDRFR",
    "response#3": "LFTEKRIEQVAIQDGKFVPTVENDLLKMAVVERHHDTGCVGLGIVKGFGLTEGAIATTVAHDSHNIVAVGISDEAMKAAIDHITQTGGGIAVVNGAGQVLHDLALPIAGLLSDKSYEEVENDLAGLLNAFKQISTADGFDPFLTLSFLTLPVIPELKLTDQGLFDFATFL"
  },
  {
    "reference": "MSTDSSPESDSEVYDPLADELHNVQLVIHVTRENIDALNAKFANLQEPPAMYLTEYQELTSKLHELKAKEHELMEQLNALQQDDDSKSQSQSEAKQQQQQQQQQQQSTLSADRTDQPHYQNQTQILQQQRQLARHAHEQTPGETGSGSQCGTLTRQPKVLLRAHLPNQQRTSVEVVAGTRLCDALMKALTLRQLEPFMCEVSTTPHSGRHIIPWNTDIGTLHVEEIYVRLLDKFPLGPHIKHQFIRKTFFSIVFCEGCRRLLFTGFYCSQCNFRFHQRCAGRVPMLCQPFSVDSYYERLLSSYPENSVGLPVGRSNAVRFNVSSRRCSSSGSSSTTTTTTTTTTNNSSSKPSANRQGRPPRISQDDRSNSAPNVCINSIRPLTNDTQRSLIMQARPPLPHPCTDHSNSTQSSPTSTLKHNRPRARSADESNKNLLSRDAKSSEENWNIQAEEILIGPRIGSGSFGTVYRAHWHGPVAVKTLKVKTPSPAQLQAFKNEVAMLKKTRHCNILLFMGCVSKPSLAIVTQWCEGSSLYKHVHVNETKFKLNTLIDIGRQVAQGMDYLHAKNIIHRDLKTNNIFLHEDLSVKIGDFGLATAKTRWSGEKQANQPTGSILWMAPEVIRMQEQNPYSFQSDVYAFGIVMYELLAECLPYRNISNKDQILFMVGRGLLRPDMTQVRSDAPQALKRLSEDCIKYNPKERPLFRPLLNMLENMLRTLPKIHRSASEPNLTQSQLQNDDFLYMCPSPKTPVNYNNFQFYNSAGNI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein contains novel RBD domains that confer a unique function or activity.2. The designed protein must possess MAP kinase kinase kinase activity, metal ion binding, kinase binding to support terminal region determination, negative regulation of macroautophagy, dorsal/ventral axis specification, ovarian follicular epithelium under certain conditions.3. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.4. The protein should be able to bind ATP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "TSPPVKKRTGGIREVLDEYNQQRRRRKYVAIKKIYVTSSPTRILNELELLNDLRGCDSVCPLITAFRHTDQVVAVLPYFRHTDFREYFRKMKVSEMQVYFRSLFTALKAVHAQGILHRDIKPTNFLYEPDRKRGVLVDFGLAEREEADCQSCLCTRDYDVRKRKLEHSAAIQNLPSVGYPKHDSRPSRRANRAGTRGFRAPEVLFKCQEQRTKIDIWSAGVILLTILSKRFPFFNSADDVEAMIEIATIFGAKRMKSCAHLHGTIFETNIPTIGQAGFALEKIILWSTCRNDAGPDGAEQPLTKEEKLAVEFLERCLELNPHKRISAEDALNHPFYT",
    "response#2": "RKLIVRLKNVQEGKQIETLVQILQDLFSLASLPSGARLFCGKNIHVPLLLVLVSYMRVASVQQVGWSLLCKLVEICPDILQKLAAPQGVGKDWDVLGVHQHILKMLKIHNANVLLSTVGLKALNLLLSSDEITLLLLEEGTDVFLLVFDAMDTFSDNEEIQKYGCKALHMLLEKVPDEQLMEFVEDKDHMIILNVFKKFPEKENLVLPALLSLHSLAGPPSNVEVLMSGNVRCYNIIVEMMKTFPKSEPIQEVSCCLLHKLTLENFFNILVLNDVHEVIVEAIEKYPANAKLQAAGLSCLALLTETIFLNRDLEENEEEEEEEEEEEKTCWFKACYKALKLHRKNTDVQEAACWALNNLLMYQRNLHERIGDEEKKFPIHREMMLAMLMHSSSKEVFQAAANALATLLEQNVNIRTILLAKGIHMNVLDMMKKHSSSAEVAESACKLLNRAFEGSFLPLDIMTVTASRVVKAMRKHKTLASVQLEALQVMLHFMTPGSLKKIKNSSLGDADFALTLKVIKNQCLLEGTHALVLDALNTFIGHPGIQKCGFQILSSVVECSGALEMLSQQGMTDTVLHTLQMYPDDQEIQCLGLGLLGSLITKKTLCIATIHVLAVILVSTLRRFKDAVEIQAQGS",
    "response#3": "RKLIVRLKNVQEGKQIETLVQILQDLFSLASLPSGARLFCGKNIHVPLLLVLVSYMRVASVQQVGWSLLCKLVEICPDILQKLAAPQGVGKDWDVLGVHQHILKMLKIHNANVLLSTVGLKALNLLLSSDEITLLLLEEGTDVFLLVFDAMDTFSDNEEIQKYGCKALHMLLEKVPDEQLMEFVEDKDHMIILNVFKKFPEKENLVLPALLSLHSLAGPPSNVEVLMSGNVRCYNIIVEMMKTFPKSEPIQEVSCCLLHKLTLENFFNILVLNDVHEVIVEAIEKYPANAKLQAAGLSCLALLTETIFLNRDLEENEEEEEEEEEEEKTCWFKACYKALKLHRKNTDVQEAACWALNNLLMYQRNLHERIGDEEKKFPIHREMMLAMLMHSSSKEVFQAAANALATLLEQNVNIRTILLAKGIHMNVLDMMKKHSSSAEVAESACKLLNRAFEGSFLPLDIMTVTASRVVKAMRKHKTLASVQLEALQVMLHFMTPGSLKKIKNSSLGDADFALTLKVIKNQCLLEGTHALVLDALNTFIGHPGIQKCGFQILSSVVECSGALEMLSQQGMTDTVLHTLQMYPDDQEIQCLGLGLLGSLITKKTLCIATIHVLAVILVSTLRRFKDAVEIQAQGR"
  },
  {
    "reference": "MVDFTNPNTLAASVLVETLFRLGLQQAVICPGSRSSPLTVALARHGGIDCVVSLDERSASFFALGHGKRTGQPVALVCTSGTAAANFLPAIIEAHYSQVPLLVLTGDRPPRLRHCRAGQTIDQTKLYGHYPQWQTELALPEPNMDYCHYLRQTALHSWQKCFWPGLGVVHLNCPFDEPLVPLEHARESLQHLAEEFSKEHFYRGLTTFTTMNRGEAISLPVNFSIFSFPSPQLDFSPLGLILVGVMPGGETPSLLTDILAIARGLGYPVLCDALCSLRNYDDGQTALITNYDFLIRCPRWAEQLVPEQIIQIGELPTSKALRHWLGTIDCPRYILNFHGENLDPLQGQTIYLSASVAQVAEYIHNQGFIPDAEQKNYAHSWLEKQRQSQTIIISALADAHTPLMVAQLAHCLPPQTNLFVANSLPVRWLEFFWPANGDHHRIFVNRGANGIDGTLSTAMGIAHRSRGETVLLTGDLSLLHDSNGFLNQSQMRGNLTIILLNNNGGGIFQTLPIAQCEDVFETYFATPQGVDFGQLCRTYGVEHKIITNLWDLKEQWPSNNSSPIRVLEIIGDRHQEAQWLKSLQAQFCCADSLIQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. thiamine diphosphate is required for the proper folding and stability of the protein.2. A protein with 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity, manganese ion binding, magnesium ion binding, thiamine pyrophosphate binding functions is required to enable phylloquinone biosynthetic process, menaquinone biosynthetic process.3. The protein should be optimized for activity in Cofactor biosynthesis, phylloquinone biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The protein should be designed to catalyze the following reaction 2-oxoglutarate + H(+) + isochorismate = 5-enolpyruvoyl-6-   hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.5. For general function, the protein need meet that Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2- succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC). The designed protein sequence is ",
    "response#1": "MEKGLIICGDDSHSEIATFATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPVSKALTQFIKKQTKAVHIVVDESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWFRMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGATLAVDAWARGTEPYTHYPTPAPSPPGPEVDALAETVRGTEHGLVVAGRLDSAAAADATRRLATHLGWPLIPDLTSRLRRGGREQPEQVPYGDLVLTSAAFREGHPPRAVLQVGGRFASKRLRLFLRDSAPEVWAVVRPDPSRIDPDHRVTHHVEAAVPAAVDALVARLEEGPRGTTWRDDWAGASERVGAVVQAHVQESDALTDPLVAALLTEEMPSEHALWPQHEDAHAYLAPLGDWLRSSEPWSPWQLTQAPCPCQPDWDELQGKRGLIVAGRIQDPVQAKAVAALAERLGWPLLADLQSQLRFDGRNLVHADLALQHSGFVAELARAEVLLQFGARLISKRLGQFIKQQPWQAYWLIDPQPARLDPDYRLRRRLLCDPAAFAAAHPVAHPHLPWHRLAERQQAVSQQVRSACERFSELGVCHRLNQLIRGQLFAGALGV",
    "response#2": "MLQTLQQQGLRHVVLCPGSRSGPLALAAAGLMRAGLITLSTAIDERSAGFYALGRSSASGVATAVITTSGTAVANLLPAAVEADRSSQPLLLISADRPLRLKNKGANQTVNQELAIACAHVYGNPSKKMRLVGLLSTALGVAHGSHKRPTVLLTGDLAFLHDSSGLRLGQQVRGSLTILLINNQGGGIFELLPIAEVGRQGPDPPFEPYFATPQLVDLALLCQAHGIPHRRVESWQELAQALDPAYLAPLGDWLRSSEPWSPWQLTQAPCPCQPDWDELQGKRGLIVAGRIQDPVQAKAVAALAERLGWPLLADLQSQLRFDGRNLVHADLALQHSGFVAELARAEVLLQFGARLISKRLGQFIKQQPWQAYWLIDPQPARLDPDYRLRRRLLCDPAAFAAAHPVAHPHLPWHRLAERQQAVSQQVRSACERFSELGVCHRLNQLIRGQLFSYLSSVEAWRKGGQTYTQRFVSPSFRDIPFCADRKGVVVIGSLSAEHAQEAKAFAQQMGWPVLADPQSGVSSDWSHYDLWLQQPKLASQLDECDLVLQFGSRIISKRLNQWINKQVSQSQQGRDVQYWFISPSLSRDNQTHLPQLHWVASPKSWVERVDVKSSSTQGWADGLLTDIAHVRAHISDEFLFSSASTLNEIALAADIEERPVRFLANRGASGIDGVVSTAAGVAAAGEGPVVLVIGDLSFYHDLNGLWAAARYRLPLVVVVVNNDGGGIFSFLPQARLDPGEFESLWGTPHGLDFRHAAALYGIPYRRASTWDDFRAAVQEALARAG",
    "response#3": "MEKGLIICGDDSHSEIATFATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNELLKETWKPDVLIRFGGMPVSKALTQFIKKQTKAVHIVVDESGQWRDPALVATEVVQASDIAFCSALIEKMPVMKKNDWFRMWQHINEKTKETLREMETYDTAFEGRVITDIVRVLPEGATLAYLAPLGDWLRSSEPWSPWQLTQAPCPCQPDWDELQGKRGLIVAGRIQDPVQAKAVAALAERLGWPLLADLQSQLRFDGRNLVHADLALQHSGFVAELARAEVLLQFGARLISKRLGQFIKQQPWQAYWLIDPQPARLDPDYRLRRRLLCDPAAFAAAHPVAHPHLPWHRLAERQQAVSQQVRSACERFSELGVCHRLNQLIRGQLFAYLAPLGDWLRSSEPWSPWQLTQAPCPCQPDWDELQGKRGLIVAGRIQDPVQAKAVAALAERLGWPLLADLQSQLRFDGRNLVHADLALQHSGFVAELARAEVLLQFGARLISKRLGQFIKQQPWQAYWLIDPQPARLDPDYRLRRRLLCDPAAFAAAHPVAHPHLPWHRLAERQQAVSQQVRSACERFSELGVCHRLNQLIRGQLFSD"
  },
  {
    "reference": "MTSHRLPEITLTPELTAAAAEAKAWPFEEARKILKRYAKTGLPETVIFETGYGPSGLPHIGTFGEVARTSMVRHAFRILTQDKVKTRLICFSDDLDGMRKVPDNVPDRAALEPYLQMPLSSVPNPFGGDYKSFAKHNNAMLCRFLDTFGFDYEFASATEYYRSGRFDTVLLKAVERYDDIMKVMLPTLGEERQATYSPFLPISPKSGRVLYVPMKKVDAKAGTITFDDEDGEETTLSVTGGKVKLQWKPDFGMRWAALGVDFEMFGKDHQTNAGIYDRICEILGGRAPEHFVYELFLDQLGQKISKSKGNGISIDEWLAYAPTESLALYNFQKPKTAKKLYFDVIPKTVDEYFTYLSAYARQEWKDRLNNPVWHIHYGNPPKADLPVTFALMLNLVSASNAESPAVLWGFISRHVPGVTPENNPELDALVGYAIRYFNDFVKPTKKFRAPDDVERAALEALDAKLGELPAGTDGNVIQNAILDVARAIERYQDHTKKSPEGGPGVSVSFFQMLYEVLIGQERGPRFGSFVALYGIDETRALIKKALAGELA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design must feature the 'HIGH' region motif.2. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "LRDWLISRQRYWGAPIPIIYCDRCGTVPVPESDLPVLLPMDVEFKPTGQSPLAECPEFVNAACPSCGGPGKRETDTMDTFMCSSWYYYRYTSPRDNDAPWDRNKVDYWLPVDQYIGGVEHAILHLLYSRFFTKVLYDLKLVSNLEPFSNLLTQGMVLKDGAKMSKSRGNVVSPEDIVARYGADTARLFILFAAPPERDLEWR",
    "response#2": "EHTIALVGASDKPDRPSYRVMKYLLDQGYHVIPVSPKVAGKTLLGQKGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQLGVINEQAAVLARDAGLNVVMDRCPAIEL",
    "response#3": "LRDWLISRQRYWGAPIPIIYCDRCGTVPVPESDLPVLLPMDVEFKPTGQSPLAECPEFVNAACPSCGGPGKRETDTMDTFMCSSWYYYRYTSPRDNDAPWDRNKVDYWLPVDQYIGGVEHAILHLLYSRFFTKVLYDLKLVSNLEPFSNLLTQGMVLKDGAKMSKSRGNVVSPEDIVARYGADTARLFILFAAPPERDLEWV"
  },
  {
    "reference": "MTSSATSPTNGVDKNKNEEMVATPANCPYQLFNQEVVWNGKWIQTRQVGFKTHTGQVGVWQSVHRNTKPVEASADGVSIIARVRKQGKLYIVLVKQYRIPCGKLCLELPAGLIDAGETAQQAAIRELKEETGYVSGKVVMESKLCFLDPGLTDDSQCLVVVDVDGDAPENQNPVQVLDSTESIEVLLVEQSALMAYVTNLDSSSIVVESTLLAYAMGIQFATI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Probably mediates the hydrolysis of some nucleoside diphosphate derivatives..2. A protein that contributes to ribose phosphate metabolic process, nucleoside phosphate metabolic process by functioning in nucleus with ADP-ribose diphosphatase activity.3. The protein must bind to Mg(2+), Mn(2+) in order to perform its enzymatic function.4. The designed protein should contain one or more Nudix hydrolase domains that are essential for its function.5. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+) binding. The designed protein sequence is ",
    "response#1": "AMPLIAEHISFLDDPVIIAPDKGALGHAREVSDILGCECDYMEKVRISPEVVETRVSDLDVEGKDAVVVDDIISTGGTIVNAAGILGKCGASSITVCCVHPVLVEDALLKIFSAGVERVIATDK",
    "response#2": "AMPLIAEHISFLDDPVIIAPDKGALGHAREVSDILGCECDYMEKVRISPEVVETRVSDLDVEGKDAVVVDDIISTGGTIVNAAGILGKCGASSITVCCVHPVLVEDALLKIFSAGVERVIATDS",
    "response#3": "AMPLIAEHISFLDDPVIIAPDKGALGHAREVSDILGCECDYMEKVRISPEVVETRVSDLDVEGKDAVVVDDIISTGGTIVNAAGILGKCGASSITVCCVHPVLVEDALLKIFSAGVERVIATDR"
  },
  {
    "reference": "MTFHAIIVAGGSGQRSGGKKQWMPLGGKPVLRWSIDAFAAADAGEIVVVVPAEDLDQARLVLTDMPVRLATGGTSRAQSVQNGLDALRDIAAMADIVMVHDAARPLLTFELIEALRQRLQTAPAAILALPVTDSLKKADGALIVDAPARTDLWRAQTPQAFRFGDITDAYAAWPADEEATDEAMVADRAGIRVEIVEGAARLQKLTYPEDFKVLEAFMSPTRMRIGQGFDAHRWGPGESVWLCGVEIVHDQTLIGHSDADAGLHALTDALLGAAGLGDIGDHFPPTDPQWKGASSDLFLKHACDLIRNKGGEIVNLDVTLICERPKIKPHREAMCERLAQITGTRLDQVSVKATTTEKMGFTGREEGLAAQAVCLITLP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should incorporate a binding pocket that specifically recognizes a divalent metal cation to enhance enzymatic efficiency.2. The protein should have a fast turnover rate for 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-   erythritol 2,4-cyclic diphosphate + CMP.3. The designed protein should have a binding site that is specific to the target a divalent metal cation, 4-CDP-2-C-methyl-D-erythritol 2-phosphate ligand.4. We require a protein that can specifically bind to the metabolites produced in the isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6, Isoprenoid biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MRVGTGFDVHRLADGEDLWLCGVKIDHHQGLAGHSDADVGLHAVVDAILGACARGDIGDHFPPSDPQWKGAASSAFVQHAVKLAGEAGYAIGNVDLTLICEAPKIGPHRGAMAVRLAELLGIPVSAVSVKATTTEKLGFTGRGEGIAAQAVTTLVVR",
    "response#2": "MRVGTGFDVHRLADGEDLWLCGVKIDHHQGLAGHSDADVGLHAVVDAILGACARGDIGDHFPPSDPQWKGAASSAFVQHAVKLAGEAGYAIGNVDLTLICEAPKIGPHRGAMAVRLAELLGIPVSAVSVKATTTEKLGFTGRGEGIAAQAVTTLVLP",
    "response#3": "MRVGTGFDVHRLADGEDLWLCGVKIDHHQGLAGHSDADVGLHAVVDAILGACARGDIGDHFPPSDPQWKGAASSAFVQHAVKLAGEAGYAIGNVDLTLICEAPKIGPHRGAMAVRLAELLGIPVSAVSVKATTTEKLGFTGRGEGIAAQAVTTG"
  },
  {
    "reference": "MRVTGIMKNYQHXWKWGIMLLGILMISSATEKLWVTVYYGVPVWKEAXTTLFCASDAKAYDTEVHNIWATHACVPTDPNPQEVVLVNVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNXXXXXXXXXXXXXXXXXXXEIKNCSFNVTLGIKSKMQKEYALFYRSDVVPIDNNSNSTNYMLISCNTSVLTQACPKISFEPIPIHYCAPAGFAILKCNDKKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEDIIIRSQNISDNAKTIIVHLNESVQINCTRPNNNTRKSIHIAPGRAFYATGDIIGDIRQAYCNVSREQWNKTLERVRGKLRSLFNKTIKFXSSSGGDPEVTMHNFNCRGEFFYCNTTELFNDTESNNTIITLPCRIKQIINMWQEVGRAMYAAPIAGNITCSSNITGLLLTRDGGLANQTGNQNDTFRPGGGDMRDNWRSELYKYKVVEIEPLGIAPTTAKRRVVQREKRAVGSTMGAASVTLTVQARQLLSGIVQQQNNLLKAIEAQQHLLQLTVWGIKQLQARVLAVERYLQDQQLLGLWGCSGKLICTTNVPWNSSWSNKTYQEIWGNMTWMEWEKEINNYSQEIYRLIEDSQNQQEKNEQELLALDKWASLWNWFDISNWLWYIKIFIMIVGGLVGLRIVFSVLSIVNRVRQGYSPLSFQTRHPAQRGPDRPEGXEEEGGERDRDRSRPLVDGFLTLIWVDLRSLCLFIYHRLRDLLLIAARIVELLGRRGWEAL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.2. A protein that can perform structural molecule activity in host cell plasma membrane, host cell endosome membrane, plasma membrane, viral envelope, virion membrane to support fusion of virus membrane with host plasma membrane, clathrin-dependent endocytosis of virus by host cell, mitigation of host antiviral defense response, positive regulation of establishment of T cell polarity, positive regulation of plasma membrane raft polarization.3. Incorporating a Di-leucine internalization motif, YXXL motif; contains endocytosis signal motif is critical for the successful design of the protein. The designed protein sequence is ",
    "response#1": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKIICTTAVPWNSSWSNKSYEEIWNNMTWTQWEREISNYTNQIYDILTESQNQQDRNEKDLLELDKWASLWNWFDITKWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTPS",
    "response#2": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKIICTTAVPWNSTWSNKSYEEIWDNMTWTEWEREISNYTSQIYDILTESQNQQDRNEKDLLELDKWASLWTWFDITNWLWYIKIFIMIVGGLIGLRIIFTVLSIVNRVRQGYSPLSFQTPSS",
    "response#3": "ISNDISNGTSIIRYHGDMKNCSFNITTELRDKQKKEYALFYKLDIVSLGNNNGNNNKTVEEYRLINCNASTIKQACPKVSFDPIPLHYCAPAGYAILKCRDANFTGKGPCKNVSTVQCTHGIKPVVSTQLLLNGSVAENETIIRSENLTNNAKIIIVQLKQPVQINCTRPNNNTRKGIGLGAGHAFYATDRILGNIRQAHCNISGQDWNKTLDDVKTQLQRHFQNKTIAFQPASGGDLEITTHSFNCRGEFFYCNTSGLFNETNGTYATNGTTITLPCRIKQIIRMWQGVGQAMYAPPIAGVISCNSNITGLLLTRDGGSGHNNNTEIFRPEGGDMRDNWRSELYKYKVVEIKPLGIAPTKARRMVVQREKR"
  },
  {
    "reference": "MHPRFETAFAQLPAALQAALAPLIADTYFPAMLSAEQVADVRRQSGLDDDALAFALLPLAAACAQTEISHFNVGAVARGLSGNLYFGGNMEFRGAAMQQTIHAEQSAITHAWMRGETGLAAITVNYTPCGHCRQFMNELNSGLTLRINLPGRAPSQLGDYLPDAFGPRDLDIKTLIFDTENHGYALKGDALTQAAIAAANRSHAPYSQSPSGLAIETRDGEIFTGSYAENAAFNPSLPPLQAALNLMSLNGYAWADIKRVALAERDDATIVQRDATAATLKALGFTNLELVTLA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein with the reaction 2'-deoxycytidine + H(+) + H2O = 2'-deoxyuridine + NH4(+), the Proton donor must be optimized to ensure efficient catalysis.2. The designed protein must be able to perform cytidine deaminase activity under specific conditions. The designed protein sequence is ",
    "response#1": "PLTDIHRHLDGNIRAQTILDLGREFNIALPATTLDTLRPHVQVTSLEPDLVSFLAKLDWGVKVLASLEACRRVAYENVEDAARNGLHYVELRFSPRYMAMTHRLPVDGVVEAVIAGVQEGCRDFQVDARLIGILSRTFGEAACQEELAALLAHREGITALDLAGDELGFPGTLFRNHFNQARDAGWHITVHAGEAAGPESIWQAIRELGAERIGHGVKAVEDPALMDYLAEHRIGIESCLTSNVQTSTVASLAQHPLKQFLEHGVLASLNTDDPAVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLTLAFLGEQEKAALIQRVAEG",
    "response#2": "LGDYLPDSFGPKDLNITTLLMDKVNHGYQIDNTSQLAQQALQAINRSHAPYSHSHSGIAVQMKSGKIFQGSYAENAAFNPSMPPLQAALIALNMAGENVMDIESAILIEKADSLLTQWDATS",
    "response#3": "LHHYLPDAFGPHNLQIDNRLFDKKAHNLFLITEDPLIQAALDAANQSHAPYSKTYSGIALQLQDQQIFQGSYAENAAFNPSLPPLQTALNYLLLNGNEVENIARAVLVEQPFRLSYR"
  },
  {
    "reference": "MTQARCPALLIAAPASGQGKTTVTAALARLHARQGRRVRVFKCGPDFLDPMILARASGAPVYQLDLWMVGEAEARRLLARAAGEADLILIEGVMGLFDGNPSAADLARRFGVPVLGVINGAAMAQTFGALAYGLAHFQPDLPFSGVLGNRVGSQRHSDILRDCLPPGMRWFGGLPRSAEFELPSRHLGLVQAEELADLDARLDAAADALRASAETDLPEPVTFEVPAPAPLQRSLEGVRIGVARDASFAFLYQANLDLLRELGAELAFFSPLQDQALPAVDSLYLPGGYPELHLGRLQGNRAMAEAIRAHHAAGKPLLAECGGMLYLLDCLEDADGERGELLGLLPGRARLQKRLTALALQEVELPEGRLRGHTFHHSTLDCAVEPLARGVCPNGRNTAEAVFRLGRLTASYIHFYLPSNPQAAAALLAPARDAD",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have ATP binding to facilitate cobalamin biosynthetic process.2. The designed protein is the optimization of the Nucleophile to achieve high catalytic efficiency for 2 ATP + 2 H2O + hydrogenobyrinate + 2 L-glutamine = 2 ADP + 2   H(+) + hydrogenobyrinate a,c-diamide + 2 L-glutamate + 2 phosphate.3. We require a protein that can specifically bind to the metabolites produced in the cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 9/10, Cofactor biosynthesis pathway.4. For general function, the protein need meet that Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of hydrogenobyrinate, using either L- glutamine or ammonia as the nitrogen source.5. The protein contains novel GATase cobBQ-type domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MLAGVSSGIGKTTISMALMSVFNNVSPFKVGPDYIDPGFHEFITGNKSYNLDIFMMGEQGVKYSFYKHHKDISIIEGVMGLYDGMDNSLDNNSSAHIARFLGVPVILVLDGVGKSTSIAAQVLGYKMLDPRVNISGVIINKVSSAKTYAIFKEAIEKYTGVKCLGFVEKNDKLNIGFMRIQEGSLKIGVALDEAFNFYYRDNIDLLNLAGAEIVYFSPVKDASLPEVDGLYIGGGYPELFAAELEANESMRQDIKKASAAGMPIYAECGGLMYLTEKISTGVPGKGTYHDASMPESTYSMVGALPGHTIMGQTRVVSYNIGTLNKDCLLGKKYNSFKGHEFHHSEIREIPEDAEFAITLSRGTGIKNGMDGLISGNTLGSYAHLLRNPEFN",
    "response#2": "MLAGVSSGIGKTTISMALMSVFNNVSPFKVGPDYIDPGFHEFITGNKSYNLDIFMMGEQGVKYSFYKHHKDISIIEGVMGLYDGMDNSLDNNSSAHIARFLGVPVILVLDGVGKSTSIAAQVLGYKMLDPRVNISGVIINKVSSAKTYAIFKEAIEKYTGVKCLGFVEKNDKLNILAIFGTASDVGKSIVATALCRMFSNAGIDVAPYKAQNMSNNSGVTPDGCEIGRAQIAQAEAARVVPTADMNPVLLKPNSDTGAQIVLQGKVCSTETAKGYFLDTSLWAEAARKSLDQLMQRHELVVIEGAGSCAEMNLYDRDFVNFRTARISGAPVILVADIDRGGVFAQVVGTLAVLPPEDRALVKGVIINRFRGDIDLFRDGVKLIETLAGIPVLGVIPYFRGDVSRLVSFITVAVADDAAFCFAYPATRERLRERADVVTFSPVAGDDLPACDGVYLPGGYPELHTDALADAPALDTLGARAADGLPVLGECGGLMALAESLTTTDGDTAEMAGVLPADVRMQDRYQALDHVELRATGDTLTAGSGATLRGHEFHYSAATVASDARFAFAVERGDGIDGDHDGLTEYRTLGTYAHVHPESP",
    "response#3": "MLAGVSSGIGKTTISMALMSVFNNVSPFKVGPDYIDPGFHEFITGNKSYNLDIFMMGEQGVKYSFYKHHKDISIIEGVMGLYDGMDNSLDNNSSAHIARFLGVPVILVLDGVGKSTSIAAQVLGYKMLDPRVNISGVIINKVSSAKTYAIFKEAIEKYTGVKCLGFVEKNDKLNISNPGRLEEEDIRLFLAVAYDNAFNFYYQDSLDVLRSLGCRILFFSPMQDAALPECDGLYLGGGYPEVFAAELSKNKSMLRSVKRAAEDEMPVYAECGGLIYLSRAFEGEGRFEFVGFLPCEVRMRRRHIGYTVNQIISRTILGDSGILRGHEFHYTECEVSDDVQFAYKMLRGSGMVSRGGENYDGIVQGATLAAYTHLHFARPDLIRDFIRKIGVIRDQVFSFYYQDNLDELSKYAELVFVNSLTDKRLPDVDALYIGGGFPEVFAEGLEKNEKLRNEIYDFCQSGNPVYAECGGLMYLGESLETSEGEFEMVGFLPLKTKMYERFQAQGYSVYRTLKPCIIAKRNQKIVGHEFHYSRPTLTGKADFAFRVERGFGIDGRRDGILKENTLGCYIHVHFLSA"
  },
  {
    "reference": "MENRWQVMIVWQVDRMRINKWKSLVKHHMHISKKAKGWLYIHHFESRHPRASSEVHIPLDEAKLVITTYWGLHTGERDWHLGHGVSIEWKLRRYSTQVDPGLADQLIHMHYFDCFTDSAIRKALLGQRVSPRCDYQAGHNKVGSLQYLALTALIKPKRTKPPLPSVRKLVEDRWNKPQKTKGHKGSHTMNGH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions; In turn, they are directed to the 26S proteasome for degradation; Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G2. The designed protein must be optimized for RNA binding.3. The protein design should emphasize Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "ETQTD",
    "response#2": "APAPEC",
    "response#3": "APAPECF"
  },
  {
    "reference": "MSNIGAITQIIGPIIDVSFENSGKLPAILNALEVTKADGQKIVLECQQHLGQYAVRTIAMHETEGLVRGMKVVDTGAAIQMPVGEAIRGRLFNVIGEAIDGLPQPKTQQKLPIHRPAPKFKDISTATEVLYTGIKVIDLLAPYVKGGKIGLFGGAGVGKTVLIMELIDNIAKSYAGLSVFAGVGERTREGNDLLREMIESGVINYGEEFRKSMEAGGWDLSKVDREALNKSHATLVFGQMNESPGARARVALTGLTAAEYFRDGNGQEKGKDVLLFIDNIFRFTQAGSEVSTLLGRMPSAVGYQPTLATEMGAMQERITSVKNGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRKIASLGIYPAVDPLESSSRILNPEVLGEIHYNTAQRVKNILQRYKELQDIIAILGMDELSEEDVKIVYRARRVQRFFSQPFHVAEQFTGLKGMRVSIEDTIKGFNMIINGELDHLPEAAFNLVGTIEQAIEKGEKMLKEAIPS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein can be targeted to proton-transporting ATP synthase complex, catalytic core F(1) and perform proton-transporting ATP synthase activity, rotational mechanism, proton-transporting ATPase activity, rotational mechanism, ATP binding.2. The protein should have a low Km value for ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate.3. For general function, the protein need meet that The catalytic sites are hosted primarily by the beta subunits.; Produces ATP from ADP in the presence of a proton gradient across the membrane4. The designed protein should have a binding site that is specific to the target ATP ligand. The designed protein sequence is ",
    "response#1": "MKSVAGTLKISLAQYRDMQAFAMFASDLDDTSRRQLDRGARLMELLKQGQFSPYPVEEQVISVWGGTTGKFDDVPVGDVLRFEGDVLEYLRSHSNVLTTIRETGG",
    "response#2": "MKSVAGTLKISLAQYRDMQAFAMFASDLDDTSRRQLDRGARLMELLKQGQFSPYPVEEQVISVWGGTTGKFDDVPVGDVLRFEGDVLEYLRSHSNVLTTIRETGE",
    "response#3": "GTRLVDTLVPIGKGQRQLIIGDEGTGRSSLAIDAVLNQKGREVRCVYVLIGQKRSTVVGTIELLRNYGALAYTTVVVGEAGSLPGLKYLAPFAGCAIAEDWMARGHDTLVVYDDLSTHAQTYRELSLLLRRPPGREAYPGDIFYLHARLLERATALNPANGGGSMTALPIVETRLGEIEAYIPTNLISITDGQIYLDSDLFAAGFKPAIDVTRSVL"
  },
  {
    "reference": "MKLRDLWAKLPPEIGSTELSEHPAWDEAVKGLCTNSHACQPGDLFIGMPGTRVDGGEFWSSAIAAGAIAALVSPQALAARPAEGEALLIPMADMTRAGAEVAAAFYGRPSQRLKMVGVTGTNGKTTTTHLIEYFLNQADQTTGLLGTLYTRWPGHQRTAVHTTPFPVELQRELSEAAAAGCQNLVMEVSSHALAQRRVWGCQFEVAVFTNLTQDHLDYHKDLDDYFEAKALLFGPDYLQGRAIVNAKDAYGQRLLERLRDRAWTYSIEDTSADLYTQDLSYSPTGVSGLLHTPVGQAAFTSPLVGQFNLENLLAAVGAALHLGVSLEQMVSALPQFAGVPGRMERVQASPGQDISVIVDYAHTPDSLDNALRAARPFISGRLICVFGCGGDRDRTKRPQMGRIAYSLSDRAIITSDNPRTEDPQRILDDVVAGIEGPLDDDQVICDRAAAIRAAVLTAQPGDGILIAGKGHEDYQILGTEKIHFDDREQAEMALKQRLA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that is capable of catalyzing reactions within the peptidoglycan biosynthesis pathway with high efficiency and specificity.2. The designed protein should contain at least one Meso-diaminopimelate recognition motif motif to achieve its intended function.3. The presence of Mur ligase central, Mur ligase C-terminal domains is critical for the interaction of the designed protein with its intended targets.4. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..5. A protein with UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity is needed to enhance cell division, cell cycle in a specific context.6. The protein have enhanced ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "MGLRLDSRAVERGDLFLAVPGHQVDGRRFIDAAIRAGAAVVLEEGDVFAVRDQSPVARIAVPELRRRVGELAARFYPRQPEALVAVTGTNGKTTVATVLFQLFEQLGESCGLISTVENKIGVRVIPATHTTPDAISLQTLFAQMRDAGCTYVFMEVSSHAVHQHRIGGVQFDGGIFTNITHDHLDYHKTFDEYISVKKSFFDHLPKTAFALTNVDDKRGAVMLQNTKALKQAYALRVPADFKGKVLENNLTGLLMQVNNQEVYFRMTGLFNAYNLLAAYGAALVWGWPLEQIGRGLSAMAAVPGRMENVVDPTGQVTAIVDYAHTPDALQNILETLAAVRQPGAQLICVVGAGGDRDKSKRPEMARIAAQLSDRVILTSDNPRTESAESILDDMSAGLISPASRASALRITDRRSAIQTAVQFARAGDIVLVAGKGHEDYQIVGQTRIHFDDREVARHALRERDAKG",
    "response#2": "MKLRDLLKGIGCTVEQGDTSIAVSSVVFDSRQVQPGSLFVAVKGTQVDGHDYIGKAIDRGAIAIVGADAGAMQKGLKEQKETKDGTSNEVTFITVEDSAKALALAAAEFYGFPSRSLCLVGVTGTNGKTSITWFLRDALDALGYHCGLMGTLGLGLKGAEIATGHTTPDPITLQRGLRDLRDAGADTVAMEVSSHALDQHRLGDTPVWAAVFSNLSRDHLDYHGDMDSYLLAKVALFTRPDLREAVINGDDLSAPVLLSRLNDGVRCIIFGGQAGATVRCVAEQPHEAGMDLRLTVGGEAINVSVPLFGAFNRSNLMAVAGIAGLTADSREVRPGWLFAALAGSKADGARFIDDALAKGATAVLVKEGTAVSAPAEVAVLSATEPRRALALMAARFLGLPVGQIAAGLAEATPPVGRMQRVGGGRLPQAIIDYAHTPDALAQAIAACREHGARYVVCVFGCGGDRDRGKRPLMGEVAGRLADSVVLTDDNPRSEPPDAIIAAIRAGIPAGVPVRIERDRRAAIVGALQAASAGDWVLVAGKGHEDYQIIGREKIHFDDREAARQALAERMAAG",
    "response#3": "MTLAQLLLRLDSRAVERGDLFLAVPGHQVDGRRFIDAAIRAGAAVVLEEGDVFAVRDQSPVARIAVPELRRRVGELAARFYGEPAASLALIGVTGTNGKTTTASLISAILGLSGAKVGLIGTIHNRIGTQVIPGNNTTPESTDLQALLRRMVREGVGACVMEVSSHALALNRVDGCEFDMAVFTNLTQDHLDFHQSMGQYLEEKLKLFKNLKVPGDKSTIKHAVINADDPAAERFAQAAGGLVHTFGIKNTADVYATNVNINTRGVNFQIDGKLGRCQLNLCLTGLFNVYNALGAFTAGVLLALGYAPDAVAAALPQLQAPAGRMQRVGGGRLPQAIIDYAHTPDALAQAIAACREHGARYVVCVFGCGGDRDRGKRPLMGEVAGRLADSVVLTDDNPRSEPPDAIIAAIRAGIPAGVPVRIERDRRAAIVGALQAASAGDWVLVAGKGHEDYQEINGERLPFDDRQVARECMARKA"
  },
  {
    "reference": "MDKIIIEGGQTRLEGEVVIEGAKNAVLPLLAASILPSKGKTILRNVPILSDVFTMNNVVRGLDIRVDFNEAANEITVDASGHILDEAPYEYVSQMRASIVVLGPILARNGHAKVSMPGGCTIGSRPINLHLKGLEAMGATITQKGGDITAQADRLQGAMIYMDFPSVGATQNLMMAATLADGVTTIENAAREPEIVDLAQFLNKMGARIRGAGTETLTITGVTHLRGVEHDVVQDRIEAGTFMVAAAMTSGNVLIRDAVWEHNRPLISKLMEMGVSVTEEEYGIRVQANTPKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMVETVFENRFQHLEEMRRMGLQSEILRETAMIHGGRQLQGAPVMSTDLRASAALILTGIVAQGVTIVNNLVHLDRGYYQFHEKLAKLGATISRSSEV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in peptidoglycan biosynthetic process, cell division, potentially with improved properties such as stability, specificity or selectivity.2. The Proton donor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The protein must exhibit the following characteristics: Cell wall formation. The designed protein sequence is ",
    "response#1": "QPRELTAEEKERLGLPVFVKPARGGSSIGISKVDSWEEFDAAIDLAFSNDNKVIVEAMIHGAEVECGVLQHADGSIVSSVPAMLNGTEDGAEGFYGFDAKYVDSTVSATIPAPLPAETIKEIRQLAIRTFNALGCDGIARVDFFATENGPVLNEINTMPGFTPISMYPKMFAAEGVSFGDLVSALL",
    "response#2": "QPRELTAEEKERLGLPVFVKPARGGSSIGISKVDSWEEFDAAIDLAFSNDNKVIVEAMIHGAEVECGVLQHADGSIVSSVPAMLNGTEDGAEGFYGFDAKYVDSTVSATIPAPLPAETIKEIRQLAIRTFNALGCDGIARVDFFATENGPVLNEINTMPGFTPISMYPKMFAAEGVSFGDLVSALL",
    "response#3": "FGPTNYYTTANGFAGFEYELAKQYADSLNVELRIVPTYSLDELFIKLNTGEVDLLASGLSITDKRLQRFRFAPSYETISQKLVFKQGNVRPRKVADLTGTLMVTSGSSYVENLEKLKQTNSELAWQESTEFDSEELLRKVLSGEIDYTIIDSNNLAINRRYYPEISIGFSINEPEPLAWMVSENSHDDILASLVEFFGTVHHDGTLLALDDKYYGR"
  },
  {
    "reference": "MLRVVCASANPHKVAEIFELIGGTLDLVARPTDLIQTKETEPTLVGNARLKAVAVCQATGLPALADDTGLEVDALNGAPGVLTARFAGANATDAENRSKLLDELKDKPRTARFRTIALLRFADGREIIAQGVCEGSIATSQRGERGFGYDSVFIPSLGNGRTFAEMSIEEKHLLSHRGLAFRSLATSLKELPTA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.2. The presence of Cyclic nucleotide-binding domains is critical for the interaction of the designed protein with its intended targets.3. The designed protein should have metal ion binding, nucleotide binding to facilitate purine nucleoside triphosphate catabolic process.4. Mg(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "MTIIIASNNAKKLNEIRAILAASGLPVEILGLADVPDAPEVVEDGDTFEANAKLKAHAAAAATGLPALADDSGLVVEALDGAPGIYSARYAGDHDDAANNAKLLHELEGVPDERRTAHFTCVIALVRHADDPTPVIAEGEWHGEILDAPRGAGGFGYDPLFWVPERDCTSAELTPQQKNAISHRGRALRALLAKLPEL",
    "response#2": "MNGDARQAS",
    "response#3": "MEEQIVLATRNAHKVSEIREILSPFGIKIISAGDLGLPEPEETGDSFIENALIKARTAADKSGLPAIADDSGLVVEALNGMPGVLSARWSGRHGDDVANNDLLLAQIADVPDERRGAAFASVCALVSPTGEEHVVQGVWPGRIAREPRGANGFGYDPLFLPDDTGKSSAELSPEEKNALSHRGRALELIAPVLRNLLG"
  },
  {
    "reference": "MGGKWSKSKIVGWSAIRERIRRTDPAAEGVGAASRDLEKHGAITSSNTAQTNSACAWLEAQEREEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEQGGLEGMIWSRKRQEILDLWVYNTQGFFPDWQNYTPGPGIRYPLTFGWCFELVPVDPKEVEEETEGENNCLLHPICQQGMEDEEREVLKWRFNSRLAFEHKAKLLRPEFFKDC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis.2. The designed protein should contain at least one Diacidic; necessary for CD4 internalization, PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Dileucine internalization motif; necessary for CD4 internalization motif to achieve its intended function.3. The designed protein should be involved in suppression by virus of host autophagy, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I by localizing to extracellular region, virion component, host cell plasma membrane, membrane and exhibiting GTP binding, SH3 domain binding. The designed protein sequence is ",
    "response#1": "MGGKWSKRSVPGWATIRERMRRAEPAAEGVGAVSRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYRGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVEPDKVEEANEGENNCLLHPMSLHGMEDPEGEVLVWKFDSRLAFHHMARELHPEYYKNC",
    "response#2": "MGGKWSKSSLVGWPAVRERMRRAEPAADGVGAASRDLEKHGALTSSNTAATNADCAWLEAQQEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQKRQDILDLWIYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPEQVEKANEGENNCLLHPMNLHGMDDPEKEVLQWKFDSRLAFHHVAREKHPEYYKDC",
    "response#3": "MGGKWSKSRMVGWSTVRERMRRAEPAAEGVGAASXDLD"
  },
  {
    "reference": "MRVLGIEGTAWAASAAVFDTNVDSDSGTDPLCTETDPYVPDSGGLHPREAAEHMAEAIPRVIERVLDADRERGTGNGDENGSQGIDAVAFSRGPGLGPCLRIVASAARAFALSRGTPLIGVNHMIAHAEIGRDRSGFDAPICLNASGANAHVLGYRNGRYRVLGETMDTGVGNALDKFARYLEWSHPGGPKVEAAAEEGEYLPLPYVVKGMDLSFSGLVSAAKEAIDKGEAIEDVCYSLQETVFAMLTEVAERALSLTDAEELVLGGGVGQNARLQDMLGEMCRARGAQFCAPEPELLRDNAGMIAALGAKMYAAGDTLPVADSRVRPDFRPDEVAVSWPVAEPGRSGNSRATARDPDDRHDPADPGESDDLGAEAVVQGAEAVVEIDRTAGRVVKRRPRKAYRHPALDERLGRERTVLEARLTSGARRLGVPTPVIEALDVRERSLTVGYVGDRDLRDLLGTDGGSSSSGDAPPATAVIERVAHHLATLHGAGIVHGDPTTRNVRVSLSETSENDPPAAGEPTLDRASLIDFGLGYHTERAEDHAMDLHVFGGSLAGTAEDADVLRGHFETAYATVGEERVLDRLREIEDRGRYQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be optimized for metalloendopeptidase activity, zinc ion binding, protein serine/threonine kinase activity.2. For general function, the protein need meet that Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37; Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine3. The protein should be designed to have high stability and activity towards adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP +   H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA.4. The designed protein should have a binding site that is specific to the target L-threonylcarbamoyladenylate ligand. The designed protein sequence is ",
    "response#1": "MRVLGIEGTAWAASAAVYDSTAETVSIFTDEYQPESGGIHPREAAEHMREAIPEVVGRARDSTDEIDVVAFSRGPGLGPCLRIVGTAGRALAGTLDVPLVGVNHMLAHLEIGRHRSGFDAPVCLNASGANAHILGYHNGRYRVLGETMDTGVGNAIDKFTRHVGWSHPGGPKVEAAAADGEYADLPYVVKGMDFSFSGIMSAAKEAYDDGVAIEDVCFSLQETIFAMLAEVSERALSLTGADELVLGGGVGQNGRLRDMLETMCAARGASFYAPEPRFLRDNAGMIAVLGAKMAAAGDDIPIEESRVRPNFRPDEVPVTWLRKSRTRKEARIMHELKKYGVRTPIIYDLREFEIVMEEIEGANLADVLNDMPSVGVKEVLRRIAQIAARIHSAGFSHGDFTTGNMILHDGDIVIIDWSGAGLNVPVPQYLGDNGAMIAYTGKIMLESGSTLPVMESGVNPSFRADQVEVTWREEPVIPSRHPEIYSARGAEAVIRFGGGSASKIRVAKQYRHPELDKKLITERTRAEARLIAEARRAGVRTPVISGITSDTIIIEHIEGLKLKDDLSIDHLVEAGRMVGRLHSAHIVHGDLTTCNFLVRDGKLWLIDFGLAATSAETEHRGVDIHVLFQVLKSTSKDYDLLKKAFIQGYQENFADADEVLDREHEIELRGRYLQEMLSVMCGERGATLYVPDRKYMGDNGAMIAYTGKIMLEHGYAREPEQSHINPNFRSDQVRVTWRRDVGEKTRGTGILPDPSTARGAEAIVTMEGDLVKKQRVKKVYRQPALDERLIAERTRAEARLISMARREGVPTPVLRDVIHDTIVMEKVKGPLLKETLLCPDIEEAGRTVGRLHAGGIVHGDLTTSNMIMRDSRCVLIDFGLSMVSREIEARGVDVHVLFQTLESTTEAYPELKDAFARGYRSVFDGADEVLMREQEIERRGRY",
    "response#2": "MLVLGIEGTAHTVGVGIVTEEKVLANVSHMYRPPEGGIHPREAANHHVQYLPSLLKEAMEKANIEPHELDGISFSQGPGLGPCLRTVATAARVMSLKLNIPIVGVNHCIAHLEIGRFSTGAEDPVMLYVSGGNTQIISYASGRYRVFGETLDIGVGNMLDKLAREMGIPFPGGPRIEKLALEGEKYIPLPYSVKGMDMAFSGILTAALNKIGKERKEDIAYSVQETVFAMLAEVTERALTHLRKDEVLLAGGVARNKRLQEMLKIMAEERGASLHAPPGELCVDNGAMIAYLGLLFLKHGKKMHLEDTQVIQKFRTDAVEIPFREFGSMDLSQTGIIQKFRTDEVDIPWVDNVKTYLKLSDDLIAKGAESNIVKSNYLGEKAVLKDRIPKGYRIAEIDDKIRRARTKEEAKLLSDAKCAGVKTPILYDINLEEKSILMEEIKGKMVKDVMDEDLAFKIGVEIAKLHAADIIHGDITSSNMMLQDGKLVFIDFGLGKYSNLDEDKAVDLLVLKKSLQSIDYNLASKYFDCVLVGYGDENMVNKISDIESRGRYTNLPEELRAKGAESDIYEATWLGREAIVKNRVSKSYRIDEIDNKIRKLRTKSEAKILSDVKKTGVRAPVLYDVDFEDKSIVMEKINGSLVKDIMHEIGEDKRRELAIAIGENIKAFHDGDIIHGDLTGSNMILIDDDLDNISDNLAIFDFGLGKYSDLLEDKAADLLVLKKSFQSIDYDIATETFDWILEAYDSNNQSKMANKIAEIEGRGRYTL",
    "response#3": "MISLGIEGTAEKTGIGIVDSDGNVLAMAGKQLYPEEGGIHPREAAEHHAKWIPQLIPQAMEEAGLSYKDIDFISFSQGPGLGPALRTVATSARTLALSLNVPIVGVNHCIGHVEIGKLDTGARNPVTLYVSGGNSQVIAYESGRYRIFGETLDIAIGNCLDHFGRETGLGHPGGPVVEKLAREGSYIDLPYVVKGMDFSFSGLLSAALRAHKNGERIEDICYSLQETAFAMLVEVTERALAHTEKDEVLLCGGVSANSRLREMMKIMAEEHYAKFYMPEMKYSGDNGVMIAWLGQLMYKAYGPLKIEDTNIIQRFRTDEVDAPLRKSRTRKEARIMHELKKYGVRTPIIYDLREFEIVMEEIEGANLADVLNDMPSVGVKEVLRRIAQIAARIHSAGFSHGDFTTGNMILHDGDIVIIDWSCSCCLRKSRTRKEARIMHELKKYGVRTPIIYDLREFEIVMEEIEGANLADVLNDMPSVGVKEVLRRIAQIAARIHSAGFSHGDFTTGNMILHDGDIVIIDWSCSCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCLLC"
  },
  {
    "reference": "MKVISSIHELRDQLRGQNRTAFVPTMGNLHEGHLSLMRLARQHGDPVVASIFVNRLQFGPNEDFDKYPRTMEADIEKLQKENVYVLFAPTEKDLYPEPQEYRVHPPHDLGDILEGEFRPGFFQGVCTVVMKLMSCVQPRVAVFGKKDYQQLMIVRRMCNQFALPTDIIAAETVRDADGLALSSRNRYLQAAERAEAPRLAAELNQVREAVLGGERDFAKIERAAMAALAARGWQPDYIAVRKRSNLLPPGPEDANAELVVLAAARLGATRLIDNLEI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for beta-alanine binding.2. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "PGARSFGGSGGYGEVQLIREHKLAVKTIREKEWFAVELVATLLVGECALRGGRTHDIRGFITPLGFSLQQRQIVFPAYDMDLGKYIGQLASLRATTPSVATALHHCFTDLARAVVFLNTRCGISHLDIKCANVLVMLRSDAVSLRPAVLADFSLVTLNSNSTISRGQFCLQEPDLESPRGFGMPAALTTANFHTLVGHGYNQPPELSVKYLNNERAEFNNRPLKHDVGLAVDLYALGQTLLELLVSVYVAPSLGVPVTRVPGYQYFNNQLSPDFAVALLAYRCVLHPALFVNSAETNTHGLAYDVPEGIRRHLRNPKIRRAFTEQCINYQRTHKAVLSSVSLPPELRPLLVLVSRLCHANPAARHSLF",
    "response#2": "YTTLEIPGARSFGGSGGYGEVQLIREHKLAVKTIREKEWFAVELVATLLVGECALRGGRTHDIRGFITPLGFSLQQRQIVFPAYDMDLGKYIGQLASLRATTPSVATALHHCFTDLARAVVFLNTRCGISHLDIKCANVLVMLRSDAVSLRPAVLADFSLVTLNSNSTISRGQFCLQEPDLESPRGFGMPAALTTANFHTLVGHGYNQPPELSVKYLNNERAEFNNRPLKHDVGLAVDLYALGQTLLELLVSVYVAPSLGVPVTRVPGYQYFNNQLSPDFAVALLAYRCVLHPALFVNSAETNTHGLAYDVPEGIRRHLRNPKIRRAFTEQCINYQRTHKAVLSSVSLPPELRPLLVLVSRLCHANPAARHSLC",
    "response#3": "YTTLEIPGARSFGGSGGYGEVQLIREHKLAVKTIREKEWFAVELVATLLVGECALRGGRTHDIRGFITPLGFSLQQRQIVFPAYDMDLGKYIGQLASLRATTPSVATALHHCFTDLARAVVFLNTRCGISHLDIKCANVLVMLRSDAVSLRPAVLADFSLVTLNSNSTISRGQFCLQEPDLESPRGFGMPAALTTANFHTLVGHGYNQPPELSVKYLNNERAEFNNRPLKHDVGLAVDLYALGQTLLELLVSVYVAPSLGVPVTRVPGYQYFNNQLSPDFAVALLAYRCVLHPALFVNSAETNTHGLAYDVPEGIRRHLRNPKIRRAFTEQCINYQRTHKAVLSSVSLPPELRPLLVLVSRLCHANPAARHSLV"
  },
  {
    "reference": "MILRLLLAFNFFPSIQGTENKILVKQSPMLVVNNNEVNLSCKYTYNLFSKEFRASLYKGADSAVEVCVVNGNFSHPHQFHSTTGFNCDGKLGNETVTFYLKNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKEQHFCPAHPSPKSSTLFWVLVVVGAVLAFYSMLVTVALFSCWMKSKKNRLLQSDYMNMTPRRPGPTRKHYQPYAPARDFAAYRS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be designed to localize specifically to membrane in order to perform its role in positive regulation of interleukin-10 production, positive regulation of T cell proliferation, positive regulation of interleukin-2 production, immune response more effectively.2. Design the protein with a signal peptide incorporated.3. For general function, the protein need meet that Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival; Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.4. The Cytoplasmic, Extracellular topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "PGLLFLLFSSLRADTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVQ",
    "response#2": "LGSSPLITVEGQEDGEMQPMCSSDGWFPQPHVPWRDMEGKTIPSSSQALTQGSHGLFHVQTLLRVTNISAVDVTCSISIPFLGEEC",
    "response#3": "LGSSPLITVEGQEDGEMQPMCSSDGWFPQPHVPWRDMEGKTIPSSSQALTQGSHGLFHVQTLLRVTNISAVDVTCSISIPFLGEEC"
  },
  {
    "reference": "MLLTISDFLFLSLTFSRYARMRDSRPWSDRKNNYSGPQFTYPPEKAPPEKLIKWNNEGSPIFEMPAEGGHIEP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in extracellular region that is critical for defense response to bacterium, defense response to fungus.2. The protein must contain a signal peptide for proper functionality. The designed protein sequence is ",
    "response#1": "KPKYRHDFYNSATEVVLTIFAKGVPAENVVVDFGEQMLSVSIEVPGEEPYHFQPRLFSKIIPEKSRYQVLSTKVEIRLAKAEQITWTSLI",
    "response#2": "KPKYRHDFYNSATEVVLTIFAKGVPAENVVVDFGEQMLSVSIEVPGEEPYHFQPRLFSKIIPEKSRYQVLSTKVEIRLAKAEQITWTSLN",
    "response#3": "KPKYRHDFYNSATEVVLTIFAKGVPAENVVVDFGEQMLSVSIEVPGEEPYHFQPRLFSKIIPEKSRYQVLSTKVEIRLAKAEQITWTSLK"
  },
  {
    "reference": "MDSEESRCFSGYDGNRFKLVILASQRAHELSSGAATLVDRQGDKNTVVALREIASNQLDLSTVFGLAVQRCRKYLEEFAGSRGIAGHSSHVSPSRSSRHTGLGKSFVDNKSTYSASFLDQDKFLSGGKDDGIEGF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Target a Polar residues compositional bias in the protein's composition for improved properties.2. The protein should be designed to localize specifically to DNA-directed RNA polymerase complex in order to perform its role in DNA-templated transcription more effectively.3. The protein must exhibit the following characteristics: Promotes RNA polymerase assembly. The designed protein sequence is ",
    "response#1": "ATNTVYVFSMKDGKYAFKTEIRSCLEHSSRPTSTLWVNMMARGSQNIKKSAIGQRIIAILPYIKQEIPIMIVFRALGFVADRDILEHIIYDFDDPEMMEMVKPSLDEAFVVQEQNVALNFIGARGARPGVTKDKRIKYAKEILQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLASLGRRELDDRDHYGNKRLY",
    "response#2": "RSPGVYYSGEFDKNGRQIFGTTVIPNRGAWLEFETDAKNISYVRVDRTRKLPLSVLVRALGFGSDSEIKEIFGDSDTLDLTLDKDVHKNPADSRVAEALKDIYDRLRPGEPKTTDSSRSLLVSRFFDPRRYDLAAVGRYKVNKKLSLKN",
    "response#3": "RSPGVYYSGEFDKNGRQIFGTTVIPNRGAWLEFETDAKNISYVRVDRTRKLPLSVLVRALGFGSDSEIKEIFGDSDTLDLTLDKDVHKNPADSRVAEALKDIYDRLRPGEPKTTDSSRSLLVSRFFDPRRYDLAAVGRYKVNKKLSLKR"
  },
  {
    "reference": "MQGRRVVVGIGGGIAAYKGIFLVRELLRRGAELRVVLTDAAARFVGPVTFAGITGRPPITDLWDPTYAGEVHVELGDWAEAMVVAPATMNLLARTANGHGDDALLATLSCARGPVLFAPAMHTRMWERPSTQRNVAHLREAGAAFVGPVEGPLASGESGVGRMAEPTEIADALAALLGPRDLAGRRVVVSAGPTHEDLDPVRYLGNRSTGRMGFAIAEAAAARGARVTLVAGPTALPSPSGVERVDIRSARELEAAIDRAWAEADAVVMAAAVADYRPAERAPGKIKKGEGPLTLELVRNPDVLAGLGARRGDATHPVLVGFALETEDVVARARGKLEKKRVDLVVANHADDGFGGRENLVTLVDAAGAEALPRMSKRAVADRLLDRVVARLADVPAGT",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should consider the endogenous proteins and enzymes present in the coenzyme A biosynthesis, Cofactor biosynthesis pathway to avoid interference with native metabolic processes.2. The designed protein should have phosphopantothenoylcysteine decarboxylase activity, FMN binding, metal ion binding, phosphopantothenate--cysteine ligase activity to facilitate pantothenate catabolic process.3. The designed protein have DNA/pantothenate metabolism flavoprotein C-terminal domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "LLVRAGHDVLPLPTPGAERFVTAETFFALARKAPSADPYPHLERADALVVAPLTANTLAKLAHGLADNILTEAALAHVGPTIVAPAMNTRMWQHSATQANLELIRARGVRVVGPALGELAEGETGLGRMSEPEEIAAVIEDLAGVTVAVSAGGTREPIDPVRYLGNRSSGRQGVAVAVAAAERGARVLLAACNVEAEVLRPAEAAGVEVRRAGSAAELSEVMREVAERADAVIMAAAVADYRVDAVSERKLTKEDGEPPVLRLVENEDIVAGLAARRRPGQVVVAFAAETAVGEELLARASRKRRRKGVDLIVVNEVGWGKGFEVAENRLLAEDAPDGLRVLVTAGGTREPIDAVRFVGNRSSGRMGVALAEEAAALGARVTVVAANVALPRSPVITWRDVESAAELEAICREEFPACDVLLMAAAVADFTPVDPQFGKLKKSGRDALRVELQPTTDVLSALSAMRSAHQTIVGFAAEHGAGALDYGREKLARKRLDAIVVNDVSAPGVGFDAVENEVTVLGADGSERAVARGSKHELATAVWDAVTDLVG",
    "response#2": "LLVRAGHDVLPLPTPGAERFVTAETFFALARKAPSADPYPHLERADALVVAPLTANTLAKLAHGLADNILTEAALAHVGPTIVAPAMNTRMWQHSATQANLELIRARGVRVVGPALGELAEGETGLGRMSEPEEIAAVIEDLAGVTVAVSAGGTREPIDPVRYLGNRSSGRQGVAVAVAAAERGARVLLAACNVEAEVLRPAEAAGVEVRRAGSAAELSEVMREVAERADAVIMAAAVADYRVDAVSERKLTKEDGEPPVLRLVENEDIVAGLAARRRPGQVVVAFAAETAVGEELLARASRKRRRKGVDLIVVNEVGWGKGFEVAENRLLLAGVTVAVSAGGTREPIDPVRYLGNRSSGRQGVAVAVAAAERGARVLLAACNVEAEVLRPAEAAGVEVRRAGSAAELSEVMREVAERADAVIMAAAVADYRVDAVSERKLTKEDGEPPVLRLVENEDIVAGLAARRRPGQVVVAFAAETAVGEELLARASRKRRRKGVDLIVVNEVGWGKGFEVAENRLLLVGSDDGLRVLVTAGGTREPIDAVRFVGNRSSGRMGVALAEEAAALGARVTVVAANVALPRSPVITWRDVESAAELEAICREEFPACDVLLMAAAVADFTPVDPQFGKLKKSGRDALRVELQPTTDVLSALSAMRSAHQTIVGFAAEHGAGALDYGREKLARKRLDAIVVNDVSAPGVGFDAVENEVTVLDSRGRVVASGPAPKWDVADAVYDLLPPRLRGRL",
    "response#3": "MTGKHVLVSAGGTREPLDAVRYLGNRSSGRMGVALAAEARRRGATVTLLAANLAVAAPAGVDVVDAPTAADLERELAARAADADVVLMAAAVADYRPEAPESGKRPKGAEPWNLTLRPTTDILQLLGERERNGQVLVGFGAETGKAGRERKRAMLAGKHLDLVVYNDVGTAGIGFDVTDNEVVLLGADGEVRASGTKAELAHRIALAVADAL"
  },
  {
    "reference": "MSLLEQLDKNIAASGGLIVSCQPVPGSPLDKPEIVAAMALAAEQAGAVAVRIEGIDNLRMTRSLVSVPIIGIIKRDLDESPVRITPFLDDVDALAQAGAAIIAVDGTARQRPVAVEALLARIHHHHLLAMADCSSVDDGLACQRLGADIIGTTMSGYTTPDTPEEPDLPLVKALHDAGCRVIAEGRYNSPALAAEAIRYGAWAVTVGSAITRLEHICGWYNDALKKAAS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should interact with enzymes and substrates within the N-acetylneuraminate degradation pathway to enhance or regulate its activity.2. The protein should be designed to catalyze the following reaction an N-acyl-D-glucosamine 6-phosphate = an N-acyl-D-mannosamine   6-phosphate.3. For general function, the protein need meet that Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N- acetylglucosamine-6-phosphate (GlcNAc-6-P).4. A protein that contributes to carbohydrate metabolic process by functioning in cytosol with N-acylmannosamine-6-phosphate 2-epimerase activity. The designed protein sequence is ",
    "response#1": "KNGAKVLGLATGSTPETLYQNFINSDLNCSNLISINLDEYVGLTPDNPQSYHYFMRKHLFDKKPFKKSYVPDGMTKDVAATCKEYDQIIKDNPIDVQLLGIGRNGHIAFNEPGTPFDIGTHEVKLTENTIKANSRFFDNEDEVPKSAICMGTANIMDAKKVVLMAFGEKKAQAIKEMIEGPVTEQVPASILQKG",
    "response#2": "EQVGAWSAQHIVNRINEFNPTADRPFVLGLPTGGTPLNTYKKLIELYKADKVSFKNVVTFNMDEYIGLDTNHPESYRTFMFENFFNHVDIQEENINLLNGNTDNHEEECQRYEDKIKSYGRINLFMGGVGNDGHIAFNEPASSLASRTRIKTLTEDTRIANSRFFDGDIAQVPKYALTIGVGTLLDSAEIMILITGHNKALALQAAVEGSVNHMWTVSALNEHDEWLNNICQELKNGAKVLGLATGSTPETLYQNFINSDLNCSNLISINLDEYVGLTPDNPQSYHYFMRKHLFDKKPFKKSYVPDGMTKDVAATCKEYDQIIKDNPIDVQLLGIGRNGHIAFNEPGTPFDIGTHEVKLTENTIKANSRFFDNEDEVPKSAICMGTANIMDAKKVVLMAFGEKKAQAIKEMIEGPVTEQVPASILQKA",
    "response#3": "EAVARMGAEQCIRLLQDKPAAVLGLATGSTPIALYAHLIQRRQQGEVSFHQVRTFNLDEYIGIAPQHPQSYRSFMQKQLFDHIDVLPENTHIPNGMGDPIAESRAYEDKIHSAGGIDLQILGLGRNGHIGFNEPTSSLSSRTRAKTLTQETIRDNSRFFSADEEQPHLAITMGIGTILDARKIMLLAAGAAKADAVKAMVEGPISAMHPASALQACLKWLLAA"
  },
  {
    "reference": "MSTPTTAGSAPTTAVPNPEAATVETGHTMSDQAQLLRIATAGSVDDGKSTLIGRLLFDSKGIFEDQLASIEATSAKRGDEYTDLALLTDGLRSEREQGITIDVAYRYFSTPRRKFIIADTPGHVQYTRNMVTGASTADVAIILVDARKGVLEQTRRHAFLSALLGIPRLIVAVNKMDLVDYDEATFEAIRTEFTDFAAKLEVHDVTFVPLSALKGDNVVNRAVDSTDSMPWYTGPALLDHLENVHIASDRNLTDVRFPVQYVIRPQRADGLDHRSFAGTVAGGIIKVDDDVVVMPSGRQSTVKAIWGPGGAELDEAAAPAAVTIALNDEIDIVRGEMIARPGNRPHQGTELEAMVCWFDDATTLQQNKRYVIKHTTRDTKAIVTGMEYRLDVNTLHRDPDSTELDLNEIGRIGLRTQTPLMYDPYRRNRDTGAFIMVDEITGNTVGAGMIIGPATTGSNVVWHAGAVQRGDRGQGRTLWLTGLSGSGKSTIASEVERLLIESGHPAYILDGDNLRHGLNADLGFSAEDRAENIRRTAEVAALFADAGVVVVCSLISPMRADRDAAREVHAKADLPFTEVFVDTPLAECEARDPKGLYAKARSGEIPEFTGVSAPYEPPLTPDLHIRPEDGTAEEVAQRILSSILGI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein with adenylylsulfate kinase activity, sulfate adenylyltransferase (ATP) activity, GTPase activity, ATP binding, GTP binding functions is required to enable phosphorylation, sulfate assimilation, hydrogen sulfide biosynthetic process.2. The protein should be optimized for activity in Sulfur metabolism, sulfite from sulfate: step 2/3 pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The designed protein is the optimization of the Phosphoserine intermediate to achieve high catalytic efficiency for adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate   + ADP + H(+).4. The GTP binding site should be located in a region of the protein that is accessible to the ligand.5. The protein must exhibit the following characteristics: With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway; APS synthesis involves the formation of a high-energy phosphoric- sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.. The designed protein sequence is ",
    "response#1": "TMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGQDIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTKRHSYLAHLIGIRNIVLAVNKMDLVDYSQAAFDKIVADYREFAHSIGIENFVAMPISGFRGDNITALSDLHHDRKDRAREIDANLVWMDPEAMKPGKVYLVKHTAQLLKGTVTSLAFRIDPETLNRQKAETLKLNEIGRVTIQLFRQIFCDEYTQNRETGSFIMIDPLTHATVAAGTVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMEVSDQFGVHLLWMDAEPLKPGRNYWIQTGTSVVGGRVSEIKHQIDVNTQEELAARELSLNAVARVDLELDRAVPFEPYALNRDLGSFVIIDRQTNGTVGAGVVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMVDRA",
    "response#2": "TMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALENDSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADCPGHEQYTRNMVTGASTADLAVILIDARKGVLVQTRRHSYLCHLLGIRNIVLAVNKMDLIDYDQARYDAIVAEYETFAKGIGIESFTAMPISGFKGDNIFRFPVQWVNRPSMDFRGFSGTVASGTVRPGDRVRVQPSGRETTVSRIVTQSGDSEQAVAGQSVTLALSDDIDVSRGDLIGEVSDQFGVHLLWMDAEPLKPGRNYWIQTGTSVVGGRVSEIKHQIDVNTQEELAARELSLNAVARVDLELDRAVPFEPYALNRDLGSFVIIDRQTNGTVGAGVVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMVRRPLNRAGEIHKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMVRRPLNRAGEINKEDATAYRSQKAKRTPATVWLTGLPASGKSTVAVALEKRLVEMGRHTYLLDGDNVRHGLNRDLGFTDADRVENIRRVAEVAKLMTDAGLIVLTAFISPFRQERAMVRELIGADEFIEVFVDTPLEVCEQRDVKGLYARARRGEIKNFTGIDSPYEPPLTPEVHLDTTTASPEEAADLIVARLA",
    "response#3": "KTMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALENDSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADCPGHEQYTRNMVTGASTADLAVILIDARKGVLVQTRRHSYLCHLLGIRNIVLAVNKMDLIDYDQARYDAIVAEYETFAKGIGIESFTAMPISGFKGDNITANSPNTRWYEGAPLVQHLEAVEINAAHDAPLLRFITCGSVDDGKSTLIGRLLYDSKRLFADQLATLEADSRRHGTQNEKLDFALLVDGLAAEREQGITIDVAYRFFDTDAAKFIVADCPGHEQYTRNMATGASTARLAVVLIDARKGVLTQTRRHSYICSLLGIDHVLLAVNKMDLVDFDRAVFERIVADYRFLADQLGIARVTAIPISGLDGDNVELLRFLTCGSVDDGKSTLIGMLLYESKMIYEDQLAAVQAASATHGTTGGGFDPALLTDGLKAEREQGITIDVAYRYFSTAKRKFIIADCPGHEQYTRNMATGASTCDLAIILVDARHGVMTQTRRHSFIVSLLGIKHVLIAVNKMDLVDYSEERFEEIKRDYREFATRLEMPDQHFVPISALKGENLSALHQLILDAVRQFEATIVWMDEQPMLRGRSYLMKIGAKTVTATIAPVKYQLSVNTLEHLAAEKLELNEIGVCELELDRNIAFDPYTENRDTGGFILIDRLANRTVAAGMVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGM"
  },
  {
    "reference": "MKTKFIFITGGVVSSLGKGLSAASIGALLEARGLRVAMQKMDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERYTTATVTRKSNFTTGQVYDTVLRKERRGDYLGGTVQVIPHITNEIKQKILDNAKGADVAIVEVGGTVGDIESLPFLEAIRQFRTDRGHENVLYVHLTLVPYITTAGELKTKPTQHSVKELREIGIQPDILLCRCDREIPHDMKGKIALFCNVREEAVITAKDVASIYEVPMNYHEQGLDERVVDYLNIWTKAPELSEWERIVSRVRAPASETTIAIVGKYVDLTESYKSLSEALIHGGIANNCKVNLTYVDSESIERHGINGIFDEVDGILVPGGFGERGSEGKVEAIRYAREHKLPFFGICLGMQMAVVEFARNVCGLDEAYSSEFREDAANPVIHIMEQQKGVKGKGGTMRLGAYPCELGKETLARRIYGQAKISERHRHRFEFNNAFRSQLEACGLVLSGVYAQENLVEIVEIKEHPWFLACQFHPEFKSRPMVPHPLFESFVGACLKSAAEGDNA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The Mg(2+), UTP binding site should be located in a region of the protein that is accessible to the ligand.2. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.3. The protein contains novel CTP synthase N-terminal domains that confer a unique function or activity.4. The designed protein must have a Nucleophile; for glutamine hydrolysis, active site active site that is highly conserved among related enzymes.5. The designed protein should have glutaminase activity to facilitate glutamine metabolic process.6. Ideal protein should have a binding affinity for key enzymes in the Pyrimidine metabolism pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "VEIALVGKYIDLPDAYLSVTEALRAGGFAHHARVNIRWVESDACTTEAGAREALRGVDGMLIPGGFGIRGIEGKLGAISWSRKNRIPLLGLCLGLQCSVIEAARSVGLEGASSTEFDPDAEHPVISTMADQVDAVAGNADLGGTMRLGSYPATLTKGSVVAEAYGSTEITERHRHRYEVNNAYRDRIAESGLQFSGTSPDGHLVEFVEYPREHHPFFVATQAHPEYKSRPTRPHPLFVAFIQAAISQS",
    "response#2": "IAMVGKYVHLRESYKSLSEALIHAGIRTRTRIKISYVDSEDIEQAGVGGLAGMDAILVPGGFGKRGTEGKIAAVRFARENNVPYLGICLGMQLALIEFARNRAGLADANSTEFAPDTPYPVVALVTEWRDADGTVTRRDEDSDLGGTMRLGGQQCRLLPGTMARRIYDNATIVERHRHRFECNNNFIATLEDAGLMVSGRTMDGSLVEMVELPEHPWFVGCQFHPEFTSTPRDGHPLFTDFVKAALAHK",
    "response#3": "LADSYVSVNHALKHAGAKIGKAVSIDWIDSETINGNVDNLSKYDGVLVPGGFGSRGAEGIIKTANYTREKNIPYLGICFGFQLAAIAFGRYVCDFKDANSTELDSNTTNPIVDLLPEQKTVSNMGGSLRLGAHEITVKNNTLAHKTYNSDKVVRRHRHRYEINTKYIDAFEKNGMIFSAESDNSKRMEMLEIPAHKFYFGVQFHPEFNSRPGYPEEAFEAFIEV"
  },
  {
    "reference": "MPPTVARNSQVLLRTIPFPSRLSPRQIRSFSRTCKHHQQHRESSFSSRLRDAWNSTPVVWKPIPIGLGIVFLGVFQLYRNNDRERRRIDDGERTEDDPGDVGKPKRRERIRPSGPWTVQIMSTIPLKAVSRWWGYMNSIDIPYYLRVPGFKLYGWIFGVNFDEIAEPDLHKYRNLSEFFYRTLKPGVRPLDPHPNALLSPADGKVVQFGVIEHGEVEQVKGVTYKVDALLGEQPTSGGRASSNTTGSKGSPDVSSDRQNHGDEEVIRDDEEFARVNGISYTLPNLLSGAAAGKQGRSWWKKPRMKDAAANADAQVSVGGSKDFPVALSDQSIKSSSSSEQEVAKDLASGPTAPRPWYYPDRPETPTALYYCVVYLAPGDYHRFHSPVSWVVESRRHFAGELYSVSPYLVKSLPGLFTLNERVVLLGRWKYGFFSYTPVGATNVGSIVVNFDKELRTNSLLTDTMADKAAEEAKERGEPYSGYAEATYESASPVLGGHALRRGEEMGGFQLGSTIVMVFEAPKGRRPTLDEGFMGTEENRKGGWQWCIEKGMKVKMGQRLGWVDSEDPS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have phosphatidylserine decarboxylase activity within mitochondrial inner membrane to regulate phosphatidylethanolamine biosynthetic process, protein autoprocessing.2. The protein design should take into account the physiological conditions of the phosphatidylethanolamine biosynthesis, Phospholipid metabolism, phosphatidylethanolamine from CDP-diacylglycerol: step 2/2 pathway.3. The Mitochondrial intermembrane topological domain of the protein should be designed to be flexible or rigid.4. The presence of pyruvate is crucial for the protein to function optimally under specific conditions.5. Target a Polar residues, Basic and acidic residues compositional bias in the protein's composition for improved properties.6. The Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity, Charge relay system; for autoendoproteolytic cleavage activity active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "MASSSKNKRRIPRCAIESDDECTTPQGSLQNKKASSHSPHHEPPAHTYNPDRKHEILSAISQTHISIGQHQFDEALHLLSFALEQRTSAFGPDHPDVXDTLYXMALINXEMGNXEXAXLLXKCYDXRXKVLGPSHPDVXLTXYXAXALXGLXXFXXAEXGXRXFXAXXGXDXLSNGXXMXEXMAEXDXRRXAAXXXYXPEXGXGXGXGEXEXXVXPXXXSXXXXQXHXXGXIDXXXVXPXTXGXNXHXDXNXPXXXHXYXXXITHXQXXXXXXXXXXXHXGXNXGXXXEXPXXXXXXXHXGXNXXXNXLHXVXHXGXTSXSXXXXTXGXXXHXXXXXXTMGGXTXXXHXXXIWXXXGXHXTHXHXTXTH",
    "response#2": "MKTRTLPVVLLILGLCCPRSWAAGASAGSGPSGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPAGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGSPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGGAPGGPGGGGGRPGGPG",
    "response#3": "MADPDAMDISDPGAGGASGGGGGGGGGSSGGGSGSGSNTGGSSGASVGGSGSGGASGGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGESGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGGGSGSSGGGNGGGNGSGGSGGGNGSGGGGSGGGSGGGSGSGGGSGGGSGGGSGGGSGSAGGN"
  },
  {
    "reference": "MKGICSVPGVKANGYKEGGKGLAVIEANGKCVGTFTRNKIQAAPVVYTEKLLSKDPDINHIIVNSGCANAFTGEQGLEDAEWMSRLIGGRNVVCSTGKIGSTINREWISMAIDNLVSGIQDGEEGCEKAADAILTTDTGPKQSVVSRSEYTVGGMAKGAGMIEPNMGTMLAFIFTDIEIPVPDMEKRFKEVVDRTFNMVVVDGDTSTNDTALLTTTGFSETPYSSVEQSLWRDIEEVCRELAKQIARDGEGATKMIEITVQGAKDKEEAKNAVKTTLNSPLVKTAVYGEDPNFGRIVAALGRSEINLRPDGFKVKINGNTVVKKGRITSFDKTKIGREMQKNKINIEIDLQNGSETITGWGCDLTPGYIEINADY",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should take into account the physiological conditions of the N(2)-acetyl- L-ornithine from L-glutamate: step 1/4 pathway.2. The desired protein should be able to perform acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate3. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "SAPKR",
    "response#2": "MGRAKR",
    "response#3": "SLLGI"
  },
  {
    "reference": "MIRVIFLDLDKTLLPEYDPEPAIPIVEELKKKGFEIVFNSSKTRAEQEYYREKLNVKGPFIVENGSAIYIPSNYFPFEVPGVKRGEYMVLELGVKVEEIRKALKELEAEYGLKYYGNSTDEEIEKFTKLPKHLIPLAKDREYSETIFLWKREGWEKDLIRKGFKVTMGSRFYAVHGNSDKGKAAKLLLDLYKRVDEVESYAVGDGENDFPMFDVVDFAFLIGDLRHENAENVTSIKDVLKKI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a high affinity for Mg(2+).2. The protein's Nucleophile must be carefully engineered to ensure that the amino acids involved in 2-O-(alpha-D-mannosyl)-3-phosphoglycerate + H2O = (2R)-2-O-   (alpha-D-mannosyl)-glycerate + phosphate are properly positioned for optimal catalysis.3. The protein must exhibit the following characteristics: Hydrolyzes mannosyl-3-phosphoglycerate (MPG) to form the osmolyte mannosylglycerate (MG)..4. The protein must bind to Mg(2+) in order to perform its enzymatic function.5. The designed protein should be involved in mannosylglycerate biosynthetic process by localizing to cytoplasm and exhibiting mannosyl-3-phosphoglycerate phosphatase activity, metal ion binding.6. The protein design should take into account the physiological conditions of the 2-(alpha-D-mannosyl)-D-glycerate from GDP-alpha-D-mannose (MPG route): step 2/2, Carbohydrate biosynthesis, 2-(alpha-D-mannosyl)-D-glycerate biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MPQFQQAKLLVVGDVMLDRYWHGAASRISPEAPVPVVQVGNQEDRPGGAGNVALNIAALGSAATLVGLVGKDDNAADLESRLTAAGVYCEFLQSVDKPTITKLRVIHQHQQLIRLDFEEIFDSADVADLHSKVTSLISSAQVLVLSDYAKGALQNVSEIIELGRKQNIPVIVDPKGSDFEKYRGATLITPNFSEFEAVVGHCKNEDELVSKGHALLSNLNLQAMLITRGEHGMTLIRPDSPELHLPARAQEVFDVTGAGDTVISVLAAALAAGDGFADATAIANLAAGLVVGKLGTAAISGPELRRALHHGQGAVKHLLTRAQLAEALAREIKQAGGKVVFTNGCFDILHYGHAHLLQYARSQGDFLILGLNTDASVRRFKGPSRPFVSEDQRAYMLSLYPFVDLIVLFDDDTPLNLIEAIRPDVLVKGGDYTPDTVVGRDVVESHGGRVAICPRLEGLSTTDLVRKIQGRGS",
    "response#2": "MPQFQQAKLLVVGDVMLDRYWHGAASRISPEAPVPVVQVGNQEDRPGGAGNVALNIAALGSAATLVGLVGKDDNAADLESRLTAAGVYCEFLQSVDKPTITKLRVIHQHQQLIRLDFEEIFDSADVADLHSKVTSLISSAQVLVLSDYAKGALQNVSEIIELGRKQNIPVIVDPKGSDFEKYRGATLITPNFSEFEAVVGHCKNEDELVSKGHALLSNLNLQAMLITRGEHGMTLIRPDSPELHLPARAQEVFDVTGAGDTVISVLAAALAAGDGFADATAIANLAAGLVVGKLGTAAISGPELRRALHQGRGPLRFVLPDERVLVCGDLMLDRYWHGDVSRISPEAPVPVVQIQRTESRPGGAANVAYNIATLGAAVTVMGLVGHDPEAHELRMLLEAQKITCHFESLTHFPTITKLRVLGHHQQLLRLDFEKPQQEGLDTVDFLAHYRAYLAQTQVVVLSDYAKGALNNLSQFMTIAREQGIPVLVDPKAKDFSRYRGATLITPNLKEFEAVVGPCTNDAELITKARELSQLHDFTAVLVTRGSRGMVLVHREHEPLILSAHAAEVYDVTGAGDTVIAVIAASLAAKTDLSQAVMLANLAAGLVVKKLGTAAVSLPELRELHSRGQSIVLTNGCFDLLHVGHVAYLEEAARLANVLVVGVNSDTSVRRLKGESRPVIAQSDRAAMLAALGCVDYVVVFDEDTPHRLIEAVRPDVLVKGGTYRPEEVVGHEIVEAYGGQVRVTGVVDGISTTNILAGRAKA",
    "response#3": "MPQFQQAKLLVVGDVMLDRYWHGAASRISPEAPVPVVQVGNQEDRPGGAGNVALNIAALGSAATLVGLVGKDDNAADLESRLTAAGVYCEFLQSVDKPTITKLRVIHQHQQLIRLDFEEIFDSADVADLHSKVTSLISSAQVLVLSDYAKGALQNVSEIIELGRKQNIPVIVDPKGSDFEKYRGATLITPNFSEFEAVVGHCKNEDELVSKGHALLSNLNLQAMLITRGEHGMTLIRPDSPELHLPARAQEVFDVTGAGDTVISVLAAALAAGDGFADATAIANLAAGLVVGKLGTAAISGPELRRALDSEQRHGQGRVPLDARVLVVGDVMLDRYWSGQTSRISPEAPVPVVHVKSNEERPGGAANVALNIASLGGTALLLGYVGNDEAGRSLANTLQARGVATRFVTLENAPTITKLRILSRHQQLIRLDFEEGFAGQDHTALLQHFSELLDAADVVVLSDYRKGTLERAQELIALARAAGKPVVVDPKAQDFATYQGATVITPNLAEFREAVGDWADEADLVRRGQALLARCGLANLLITRSEQGMTLLRDGVEPYNLPTRAREVFDVTGAGDTVVAVLAAALAAGLGVEQAMALANLAAGIVVGKVGTATVSVPSYETARVLVCGDLMLDRYWHGDVSRISPEAPVPVVQIQRTESRPGGAANVAYNIATLGAAVTVMGLVGHDPEAHELRMLLEAQKITCHFESLTHFPTITKLRVLGHHQQLLRLDFEKPQQEGLDTVDFLAHYRAYLAQTQVVVLSDYAKGALNNLSQFMTIAREQGIPVLVDPKAKDFSRYRGATLITPNLKEFEAVVGPCTNDAELITKARELSQLHDFTAVLVTRGSRGMVLVHREHEPLILSAHAAEVYDVTGAGDTVIAVIAASLAAKTDLSQAVMLANLAAGLVVKKLGTAAVSLPELRR"
  },
  {
    "reference": "VNWKKILGKIIKVAK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should be able to enhance the efficiency of defense response to bacterium.2. The protein must exhibit the following characteristics: Has cytotoxic but no hemolytic activity; Penetrates into cells once membrane has been permeated ; Insertion into membranes involves Trp-3. The designed protein sequence is ",
    "response#1": "LAQRLRAGLSTTGPAAALIASSISLAISPLAFLRVADNFNRSKEIGEFAERFKKLGYDGDKLLSEFYHEAGTIDASITTISTALSAIAAGTAAASAGALVGAPITLLVTGITGLISGILEFSKQPMLDHVASKIGNKIDEWEKKYGKNYFENGYDARHKAFLEDSFSLLSSFNKQYETERAVLITQQRWDEYIGELAGITGKGDKLSSGKAYVDYFQEGKLLEKKPDDFSKVVFDPTKGEIDISNSQTSTLLKFVTPLLTPGTESRERTQTGKYEYITKLVVKGKDKWVVNGVKDKGAVYDYTNLIQF",
    "response#2": "LAQRLRAGLSTTGPAAALIASSISLAISPLAFLRVADNFNRSKEIGEFAERFKKLGYDGDKLLSEFYHEAGTIDASITTISTALSAIAAGTAAASAGALVGAPITLLVTGITGLISGILEFSKQPMLDHVASKIGNKIDEWEKKYGKNYFENGYDARHKAFLEDSFSLLSSFNKQYETERAVLITQQRWDEYIGELAGITGKGDKLSSGKAYVDYFQEGKLLEKKPDDFSKVVFDPTKGEIDISNSQTSTLLKFVTPLLTPGTESRERTQTGKYEYITKLVVKGKDKWVVNGVKDKGAVYDYTNLIQG",
    "response#3": "LAQRLRAGLSTTGPAAALIASSISLAISPLAFLRVADNFNRSKEIGEFAERFKKLGYDGDKLLSEFYHEAGTIDASITTISTALSAIAAGTAAASAGALVGAPITLLVTGITGLISGILEFSKQPMLDHVASKIGNKIDEWEKKYGKNYFENGYDARHKAFLEDSFSLLSSFNKQYETERAVLITQQRWDEYIGELAGITGKGDKLSSGKAYVDYFQEGKLLEKKPDDFSKVVFDPTKGEIDISNSQTSTLLKFVTPLLTPGTESRERTQTGKYEYITKLVVKGKDKWVVNGVKDKGAVYDYTNLIQCL"
  },
  {
    "reference": "MALSRLSSSSSSSNGSNLFNPFSAAFTLNRPISSDTTATLTIETSLPFTAHNCDPPSRSVTTSPSELLSFFRTMALMRRMEIAADSLYKANLIRGFCHLYDGQEAVAVGMEAGTTKKDCIITAYRDHCTFLGRGGTLLRVYAELMGRRDGCSKGKGGSMHFYKKDSGFYGGHGIVGAQVPLGCGLAFGQKYLKDESVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTATWRSAKSPAYFKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERVRKLLLSHDIATEKELKDTEKEVRKEVDEAIAKAKDSPMPDPSDLFSNVYVKGYGVEAFGVDRKEVRVTLP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in mitochondrial matrix that is critical for glycolytic process.2. The protein must exhibit the following characteristics: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). The designed protein sequence is ",
    "response#1": "NIPDLIDFKSLLKEQNKKMSTTIAFVRVLNLILKNHSIKNLIVPIIADEARTFGMEGLFRMIGIYSSIGQKYVPQDREQLAYYKEEKKGQILQEGINELGAASSWLAAATSYSTNDFPMIPFYIYYSIFGFQRIGDLFWAAGDQQARGFLIGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCVSYDPAFAYEVAVIIQDGLRRMYGPLQENIYYYITTINENYYMN",
    "response#2": "GGHLSSFQSSATIYEVCFNHFFRSKNDEDGGDLVYFQGHIAPGIYARSFLEGRLSKKQIDNFRQEVDGKGLSSYPHPKLMPNFWQFPTVSMGLGPLCAIYQAKFLKYLQNRELKNTSKQTVYAFLGDGEMDEPESKGAISIAVREKLDNLIFVINCNLQRLDGPVVGNGKIVNELESFFYGAGWKVIKVIV",
    "response#3": "GGHLSSFQSSATIYEVCFNHFFRSKNDEDGGDLVYFQGHIAPGIYARSFLEGRLSKKQIDNFRQEVDGKGLSSYPHPKLMPNFWQFPTVSMGLGPLCAIYQAKFLKYLQNRELKNTSKQTVYAFLGDGEMDEPESKGAISIAVREKLDNLIFVINCNLQRLDGPVVGNGKIVNELESFFYGAGWKVIKVIQ"
  },
  {
    "reference": "MTRLTLAIDAMGGDFGPCVTVPASLQALESNPHLHLLLVGDPDIITSSLATADSALRARLQIIAAESVIASDARPSQAIRASRGTSMRIALEQVKEGHAQACISAGNTGALMGLAKLLLKPLDGIDRPALMTVLPHQQHGKTVVLDLGANVDSDSAMLVQFAVMGAVVAEEVLAISRPRVALINIGQEDSKGLTSIRDAAAILRAAAEINFIGYLEGNDLLTGKTDVLVCDGFVGNVTLKTMEGVVRMFLSLLKSQGEGKKKAWWLRLLGRILQKRLAKKFGHLNPDQYNGACLLGLRGTVIKSHGAANQRAFTVAIQQAEQAVQRQIPQRIAARLEAVLPKSD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in fatty acid biosynthetic process.2. The protein must exhibit the following characteristics: This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA.; Catalyzes the reversible formation of acyl-phosphate (acyl- PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP).3. A protein that is capable of catalyzing reactions within the Lipid metabolism pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "IVITGISGRLPESTNIEEFRKNLFEGVDMVNDEPRRWSKGLYGLPERNGKIKEEDLENFDQTFFGIHQKQADGMDPQMRMLLECTYEAIIDAGLNPAEIRGTRTGVYVGASSADSENYQLSDPDRVDGYGLIGCARAMFPNRISFSFDLKGPSYCVDTACSSSLYALSQAFADMNSGKCDAAIVAGSNLCLRPTMSLQFKRLSMLSAEGKCKAFDESGSGYVRSDAVVVILLQRVSVAKRVYATILNARTNVDGHKEQGITFPNGQMQNQLMRETYGEINLDPAEVNYVEAHGTGTKVGDPQEVNSITDFFCKNRKSPLLVGSVKSNMGHSEPSSGLCSIAKVLLAMEQNVLPPNLHYKNPNPDLYGLLDGRLKVVDKPTPWEGGIVGINSFGFGGANAHVILKSNPGDA",
    "response#2": "YATILNARTNVDGHKEQGITFPNGQMQNQLMRETYGEINLDPAEVNYVEAHGTGTKVGDPQEVNSITDFFCKNRKSPLLVGSVKSNMGHSEPSSGLCSIAKVLLAMEQNVLPPNLHY",
    "response#3": "IVITGISGRLPESTNIEEFRKNLFEGVDMVNDEPRRWSKGLYGLPERNGKIKEEDLENFDQTFFGIHQKQADGMDPQMRMLLECTYEAIIDAGLNPAEIRGTRTGVYVGASSADSENYQLSDPDRVDGYGLIGCARAMFPNRISFSFDLKGPSYCVDTACSSSLYALSQAFADMNSGKCDAAIVAGSNLCLRPTMSLQFKRLSMLSAEGKCKAFDESGSGYVRSDAVVVILLQRVSVAKRVYATILNARTNVDGHKEQGITFPNGQMQNQLMRETYGEINLDPAEVNYVEAHGTGTKVGDPQEVNSITDFFCKNRKSPLLVGSVKSNMGHSEPSSGLCSIAKVLLAMEQNVLPPNLHYKNPNPDLYGLLDGRLKVVDKPTPWEGGIVGINSFGFGGANAHVILKSNPIKGIKPRVHI"
  },
  {
    "reference": "MDALQLGSYTFSSRLILGTGKFSNATVMLEAVRASGAQLITVALRRFNREQAEDDLFGPLSAIEGVTLMPNTSGASTAAEAIRAAHIARELSGSPFIKVEIHPNPQHLMPDPIETLEAARVLASEGFLVMPYITADPVLAKRLEDVGCASVMPLGSAIGSGQGVATAAMIEIIIRESGIPVIVDAGLRSPSEAANAMEMGCSAVLVNSAVAVAGNPPEMAEAFAEAVAAGRKAFRAGLMQKSGSAIATSPLTAFLGAKG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in thiamine diphosphate biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.2. We require a protein that can specifically bind to the metabolites produced in the thiamine diphosphate biosynthesis pathway. The designed protein sequence is ",
    "response#1": "DPSGGAGIQADLKSIAANGGYGMAAITALTAQSTQGVRSVHVPPADFLGEQLDALADDVDIDAVKIGMLADAEIIRTVREWLDRVRPETVVLDPVMVATSGDRLLDAAAEDALRGLLPLADVVTPNLPELAVLAGEPQAGDWEQALEQAARLSERYGVLVLVKGGHLDSADAPDALVGPDGALAAFSDPRIVTDNTHGTGCSLSSALASRRARGADWPTAVAEARAWLRESIAAGSDLDVGRGHGPV",
    "response#2": "DPSGGAGIQADLKSIAANGGYGMAAITALTAQSTQGVRSVHVPPADFLGEQLDALADDVDIDAVKIGMLADAEIIRTVREWLDRVRPETVVLDPVMVATSGDRLLDAAAEDALRGLLPLADVVTPNLPELAVLAGEPQAGDWEQALEQAARLSERYGVLVLVKGGHLDSADAPDALVGPDGALAAFSDPRIVTDNTHGTGCSLSSALASRRARGADWPTAVAEARAWLRESIAAGSDLDVGRGHGPV",
    "response#3": "DPSGGAGIQADLKSIAANGGYGMAAITALTAQSTQGVRSVHVPPADFLGEQLDALADDVDIDAVKIGMLADAEIIRTVREWLDRVRPETVVLDPVMVATSGDRLLDAAAEDALRGLLPLADVVTPNLPELAVLAGEPQAGDWEQALEQAARLSERYGVLVLVKGGHLDSADAPDALVGPDGALAAFSDPRIVTDNTHGTGCSLSSALASRRARGADWPTAVAEARAWLRESIAAGSDLDVGRGHGPA"
  },
  {
    "reference": "MGALMIELLGKSLVELQALFEEHKIQKFRAKQLIDYIYHRHIFVFQDMTQFPKTLRDWLDSNCIISIPKVITQSVSPDGKTQKLLLELTDHSRIEAVLMEQYYGNSVCVSSQVGCAMGCVFCASTQGGLFRDLSVSEIVGQVVLFSALKQEDIHSLVVMGAGEPLQNYDNVLQALKLIHDPMTFDISYRKMTISTCGWVPNIYKLADEDLPITLALSLHATTDETRRKIMPVGSRYKLDEVLDAVKYYYEKTQRRITFEYILIDSINVSLEEAHELGNIGKAFPNCHVNLIPVNGNEHINLYKPSSKHMNIFKDIVASYGVSVTIRKEMGDAIQAACGQLKVAHGRKEEIHE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be optimized for tRNA (adenine-C2-)-methyltransferase activity.2. The designed protein should have a high specificity for [4Fe-4S] cluster binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "SSQVGCSLNCTFCATARLKRMRNLNPDEIYDQVVSIDQQSRLFEGHPLTNIVFMGMGEPLLNYDNVIKAIDMIISDEGLGMSPRRITLSTVGLPKMIKQLADDEVKFNLAVSLHSAIDEVRSEMMPVNRTHNLAELLESLQYWYAKTAKRITFEYVVWKGINDKKR",
    "response#2": "MTKGVTMCLSSQVGCAQACSFCMTAQMGFIRNLSPGEIVGQAFAIAHDQQLEDPFNIVMMGMGEPLLNLNNLMKAIDLFTDDLGFGLSPRRITVSTSGHVRNLLKLGQYPKLPRIAVSLNASTDEQRNQLMPVNLRWNIAELIDACKQLPIPPRERVTLEYVLIKDLNDTPADAKRLVRLVANLRCKINLIPFNETEGLSYRQPSLQVIEAFQAILNRSQIRNTVRWSKGE",
    "response#3": "SQSGCPLQCTFCLTAQLGLLRSLTAGEIVEQIIIALNDAYGVGVKPPRGINLVGMGAGEPFLNFDELMKALGIMADPGGLFIVPNRVTISTAGVVPRIRELATMPARPHLAISLAAATDELRDELMPINKKWPLEDLLSACKEFEKSLKPGERVTFEYVMLNGVNDSDQQR"
  },
  {
    "reference": "MAHEILTPAEMALVDRAAIEGGPLSGIDLMHNAGHAVAALILERFSGAAKVDVLCGPGNNGGDGYVVAGLLSDAGMNVALYAQGRPRVGSDAAIAARECPIQPQPLADYEPEAGSVIVDALFGAGLSKPLSHELCRIAEATSSRTVVAVDLPSGVSGETGVILGAAFRAAITVTFTRKKPGHLLLPGRDFCGEVVVADIGIGDRTVAAIGSACVENTPAAWIGHFPAPNINSYKYSRGHVGVFSGGPAATGAARLAAMGAARIGAGAVTLLSPSASTAINAAHLTSIILREADTLEEVLAFLEARRPHSLVYGPGLGAEADAGDFLLAMLAKAGGLPSLVLDADGLSTFAGRPELLFDALHRDGAPATVLSPHEGEFRRLFPDIAGESQISKLEKARAAAKRSGAVIIYKGPDTVIAAPSGYAAINGNGTPLLATAGSGDVLSGICAGLLAQGMPPFEAACAAVWAHADAALRFGPGLIAEDLPLALLPTIRELLPARQAERWNTVFGKPDAQTKG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must possess ATP binding, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADHX epimerase activity to support nicotinamide nucleotide metabolic process under certain conditions.2. The designed protein should contain one or more YjeF N-terminal domains that are essential for its function.3. The designed protein should have a high specificity for AMP, (6S)-NADPHX, K(+) binding, with minimal off-target effects.4. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "LIIAGSYDMPGAAVIASAAAVNSGAGLIKLAFPDKAYPAVTSSCPEKILLPLMTNNNGKISSQNIKKIEDELGKCDAVLIGCGMGCDHDTAAIAETVLKSSAVPVIIDADGINALKDNINVIKSCLSPVVITPHPGEAARILDCTVGEIEKDRKAACKAIFEKTGAVVVLKGSRTIVTANGIDFYVNLTGNPGMATAGSGDMLSGIILSFICQKIKPFSAAVCGVFVHGMAGDIVKSKYSMMGTTPTRMTEELI",
    "response#2": "MKSIDRRAITQYGIPGVVLMENAGRGVVTVIEKSFAVGRGAEVLVVCGVGNNGGDGMVVCRHLLRRGASPRCFLVGKKSRMKGDAKTNLHILEKCGIDVVEIEDRIPRQFTRSLGRSVLVVDALFGTGFRGKLKGCALEVVERINRSRLPVVSIDCPSGLNVDTGCVDGSCVEATATVTMCVPKVGLVFYPGRRWVGELWVVDIGVPEQAIPERLKWGHKGSFGKLLVVAGARYYYGAPYYSSYSFLKAGGGYSRLAAPKSVIPVLASRCSEVVYHPMDETTEGSLSMGNYESILRIIDEHGVDIVVLGPGTSLNEETQELIRKLAGAINKPLLIDGDGLTAISKDPSIIKKRKAGTILTPHPLEFTRLTGVDLSDLREDPVGYIRRASQDLNSYIVLKGAHSLIANPDGRVYVNMTGNPGMAKAGMGDVLNGVITAMYGVGFRDLGEATRMGVLVHGLAGDLAAEEVGEDGVTPEAVMEYLPRAVLLESHDIHMPPRDPAGHKTTFGWVAIVAGSSGLTGAGAMASLACLRCGAGMATLCLPESLNAALEAKLTEVMTYPLPETDAKTLSLNALELMAPVLKKADVVAVGPGLSTHPETEQLVRSLIEKVDLPMVVDADGLNALSGKLRILRRRKAATILTPHPGEMARLTKMGVPEIMANKPGVATRFAGENRVTTVLKGAPTIIASPKGEVLINSTGSSALATAGSGDVLTGVIAGLLAQGLSSTDAAATGVFLHGLAGDIMAERRTEYGVIATDLLESIPEAIELVLD",
    "response#3": "LLPRRRNSHKGSFGNVAVIGGAPGMTGAPILAARTALKCGAGRVLVGFADNPPAYDSMQPELMCRHADDLDFSAAVLVLGPGMGMADSAQALLTRALDALSWLVLDADALNLLARQPQLQIQAAQRQHAILMTPHPLEAARLLGLSTSEIQADRVTAARKLACRFNAVVVLKGVGSIIARADGAVAVNPTGNPALATAGSGDVLSGICGALLAQGWPQWEAALAATWLHGRAADQLVEQGMGPVGLTAGELIPR"
  },
  {
    "reference": "MPNARVEDAIADIKAGRFVIVVDDYDRENEGDLVMASEMVTPDVINFMAKNARGLICVSLTAERLEQLKIPMMVTENTSPYSTGFTVSVEARKKVTTGISAADRAATVQVLIDTAATREDIAMPGHMFPLRAREGGVLVRAGHTEASMDLARMAGLYPSGVLCEIMNDDGTMARLPQLEAKAAEWGIKIISVKDIISYRRRTEKMVKKITEAELPTSHGLFKAVAYKSQFDADEHIALVMGDISGPEPVLVRVHSECITGDVFGSKRCDCGEQLDMALNQIAKAGRGVLLYMRQEGRGIGLHNKLCAYALQDKGLDTVEANIELGFAPDLREYGIGAQILVDLGLSKIRMMTNNPKKVVGLEGYGIKVVETVPIIVEPNNYNRHYLETKQKKMGHILHVDKVN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a high specificity for Mg(2+), D-ribulose 5-phosphate, Zn(2+), GTP binding, with minimal off-target effects.2. For general function, the protein need meet that Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.3. The presence of Zn(2+) is necessary for the protein to function in the desired environment.4. The protein design should take into account the physiological conditions of the Cofactor biosynthesis pathway.5. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile; for GTP cyclohydrolase activity, Proton acceptor; for GTP cyclohydrolase activity to enable effective catalysis of GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.6. The presence of GTP cyclohydrolase II domains is critical for the interaction of the designed protein with its intended targets.7. The designed protein should be optimized for GTP binding, zinc ion binding, manganese ion binding, GTP cyclohydrolase II activity, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity activity in order to improve riboflavin biosynthetic process. The designed protein sequence is ",
    "response#1": "MVRVLETRLPTRYGDFRLVLYGTTVNEEHHLAIVKGDVEGKKDVLVRVHSSCVTGDAIGSLRCDCGEQLRAALERIESEGEGVVLYMHQEGRGIGLENKLQAYVLQDGGLDTVEANVHLGFKPDPRDYGIGCQILVDLGLSSLRLLTNNPQKRVALEAYGLSFSDIPHEDVPDTPERVTEADLPTRYGKFRIYGYVNLINREHHVALAMGNVNDGRPVLCRIHSECLTGDCFGSRRCDCGEQLDTSMRMIAKEGRGVLIYLRQEGRGIGLINKLRAYALQDEGYDTVEANLKLGFAADLREYMTGAQILHNLGAETIRILTNNPQKIGDVSEYGITIAERIPSYPDHRVATNLPTIYGNFKLHLYRSYVDDYLHIALCHGDIGSMDGLPTPQHQEAVLVRVHDECLTGDIFGSLRCDCGEQLHNALKMIQKEGKGVVLYMRQEGRGIGLENKLHAYALQEKEGLDTVEANERLGFLPDKRDYGIGAQILKDLGITKMRLLTNNPKKFKALEGYGLEIVERERSVEVDLPTRYGHFRLINYRSLVDGKNQLALVKGEIKSEESILVRVHSECLTGDVFGSMRCDCGDQLHLAMERIAEEGTGVLLYMNQEGRGIGLENKLKAYQLQDEGRDTVEANEELGFKADLRDYGIGAQMLYDLGVRNIRLLTNNPRKVIGLKGYQLEIVERIP",
    "response#2": "MKLTIELPTEHGNFTLTAYKQTNTGRDHIALYKGSWEPEEEILVRVHSSCITGDIFGSCRCDCGKQLAQAMKNVESEGRGVIVYMNQEGRGIGFINKMKAYKLQQEGYDTVQANEKLGFKADERDYGVGAQILRDLGVRKLKLMTNNPKKRAGLIGYGLEIVENKRQDTKLYPRVATNLPTIYGNFKLHLYRSYVDDYLHIALCHGDIGSMDGLPTPQHQEAVLVRVHDECLTGDIFGSLRCDCGEQLHNALKMIQKEGKGVVLYMRQEGRGIGLENKLHAYALQEKEGLDTVEANERLGFLPDKRDYGIGAQILKDLGITKMRLLTNNPKKFKALEGYGLEIVERREVEVRMPTEFGEFTMYGYSNRVDAAEHVALVMGKPGPEKPVLVRVHSECLTGDVFTSRRCDCGSQLHAAMRQIAEEGEGVILYLRQEGRGIGLLNKLRAYKLQDEGHDTVEANRKLGFKADLRDYGIGAQILRDIGLRKLRLLTNNPKKIIALDGYGLEVVERERSVEVDLPTRYGHFRLINYRSLVDGKNQLALVKGEIKSEESILVRVHSECLTGDVFGSMRCDCGDQLHLAMERIAEEGTGVLLYMNQEGRGIGLENKLKAYQLQDEGRDTVEANEELGFKADLRDYGIGAQMLYDLGVRNIRLLTNNPRKVIGLKGYQLEIVERIYKTNRVATNLPTIYGNFKLHLYRSYVDDYLHIALCHGDIGSMDGLPTPQHQEAVLVRVHDECLTGDIFGSLRCDCGEQLHNALKMIQKEGKGVVLYMRQEGRGIGLENKLHAYALQEKEGLDTVEANERLGFLPDKRDYGIGAQILKDLGITKMRLLTNNPKKFKALEGYGLEIVER",
    "response#3": "MKLERVASTVLPTRSFGELEVHAYESSVDPFPAVALTKGDLATDEPVLVRVHSSCLTGDLLESLRCDCGDQLHLALSRIHNEGRGVLVYLQQEGRGIGMVNKIRAYALQDQGADTVEANTQLGFKPDLREYGIGAQILADLGIKRIRFMTNNPAKVAALEGYGLEIVEWVPPEVDEEDVTRVATNLPTIYGNFKLHLYRSYVDDYLHIALCHGDIGSMDGLPTPQHQEAVLVRVHDECLTGDIFGSLRCDCGEQLHNALKMIQKEGKGVVLYMRQEGRGIGLENKLHAYALQEKEGLDTVEANERLGFLPDKRDYGIGAQILKDLGITKMRLLTNNPKKFKALEGYGLEIVERRVLETRLPTRYGDFRLVLYGTTVNEEHHLAIVKGDVEGKKDVLVRVHSSCVTGDAIGSLRCDCGEQLRAALERIESEGEGVVLYMHQEGRGIGLENKLQAYVLQDGGLDTVEANVHLGFKPDPRDYGIGCQILVDLGLSSLRLLTNNPQKRVALEAYGLSFSDIPPEVDEEDVAEAHLPSVYGGEWKAIAFENDVDHLDHIALVKGEFAKGESILVRVHSECLTGDVLGSKRCDCGEQLHRAMEMIEREGKGVILYMRQEGRGIGLINKLRAYELQDRGRDTVEANLELGFKADLRDYGIGAQILLTLGITKIRLLTNNPKKLIGLQGYGIDIVDRPPSRVDDEDVRRVATNLPTIYGNFKLHLYRSYVDDYLHIALCHGDIGSMDGLPTPQHQEAVLVRVHDECLTGDIFGSLRCDCGEQLHNALKMIQKEGKGVVLYMRQEGRGIGLENKLHAYALQEKEGLDTVEANERLGFLPDKRDYGIGAQILKDLGITKMRLLTNNPKKFKALEGYGLEIVER"
  },
  {
    "reference": "IGAGVGRDGTIAATKGKAKTLAELIXMYDS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Participates in sulfate respiration coupled with phosphorylation by transferring electrons from the enzyme dehydrogenase to ferredoxin.2. The protein design should aim to mimic or enhance the function of natural proteins involved in anaerobic respiration, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "MPNWGQVMFQDAASSVMLQLVSFHDHALLVLTLVLTVVGYALLALMLNKQVNRYIMEAQTVETIWTILPALILLVLALPSLRILYITDEVK",
    "response#2": "MPNWGQVMFQDAASSVMLQLVSFHDHALLVLTLVLTVVGYALLALMLNKQVNRYIMEAQTVETIWTILPALILLVLALPSLRILYITDEVN",
    "response#3": "SVMLQLVSFHDHALLVLTLVLTVVGYALLALMLNKQVNRYIMEAQTVETIWTILPALILLVLALPSLRILYITDEVSQPSITVKTIGHQWYWSYEYTDFLNVEMDSYMLPTSDLLPGDYRLLEVDNRMVVPMQLEIRMLITAADVIHSWTVPALGVKVDAVPGRLNQIGFTTTQPGVFYGQCSEICGANHSFMPIAVEAINTKSFMSWY"
  },
  {
    "reference": "MPAFSQATDLSAWKELQEHHTAVGRNIVLKEAFEKDPQRFEKFSRTFKNTVDNSDILFDFSKNFLTEETLSLLVKLAKEANVEELRDAMFKGEHINFTEDRAVYHAALRNVSNEPMQVDGKSVVEDVNSVLEHMKEFSEQVRSGEWKGYTDKKIDTIINIGIGGSDLGPVMVTEALKPYGAPGMKLHFVSNIDGTHIAEALKDSNPETTLFLIASKTFTTAETTTNANSAKKWFLETAKDESHIAKHFVALSTNEAEVTKFGIDKKNMFGFESWVGGRYSVWSAIGLSVALYIGYDNFHQFLAGAQAMDKHFREAPLEQNIPAIGGLLSVWYSDFFGAQTHLVAPFDQYLHRFPAYLQQLSMESNGKAITRSGEYVKYTTGPILFGEPATNAQHSFFQLLHQGTKLIPSDFIMAAESHNPVEGGKHQRMLASNFLAQSEALMVGKTPEQVKTEGAPDNLVPHKTFLGNRPTTSILAQKITPSTLGALIAYYEHLTFTEGAVWNINSFDQWGVELGKVLAKKIQQELETSGAGAGHDASTSGLLAAFKQKANLA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be designed to catalyze the following reaction alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate. The designed protein sequence is ",
    "response#1": "PDRQLMLFAQDVLNRNPGAKVIFDVKSTRLLAPWIKEHGGKAIMEKTGHSFIKSAMKETGAPVAGEMSGHIFFKERWFGFDDGLYAGARLLEILSASDNPSEVLNNLPA",
    "response#2": "LIIIHGGGPTAVLNCSLYGAVREAQASGLVEHVYGAVGGTGGLLKEKLLDFGSVPEEELERLPTTPATAIGTSRDALEAEDYARMADILSEKHIRYVLMNGGNGTMDACGKLAAVCRDRGISVMGIPKTMDNDIAVTDHCPGFGSVARYMAGTVRELGVDVRSLPIHVVVLEALGRNAGWTTAAAALAREQEGDAPHLLYPPERPFHEDEFLDRVGDMYRKLGGVVVVASEGLHDDEGKPIVPPIFQTGRSTYFGDVSSHLAQLVVRRLGIKARSEKPGILARASIAWQSPVDAAEAEEAGRAACRILAERISAGDPVSALR",
    "response#3": "PDRQLMLFAQDVLNRNPGAKVIFDVKSTRLLAPWIKEHGGKAIMEKTGHSFIKSAMKETGAPVAGEMSGHIFFKERWFGFDDGLYAGARLLEILSASDNPSEVLNNLPA"
  },
  {
    "reference": "MFEIIPILAFEDNYIWLLHQHGHALVVDPGDAHPVLEILDARGLQLRAILVTHHHQDHTGGVEELIQATSAQVFAPAKEQFSFPHHPVTAGDRLDIPGIALSLSVLDVPGHTVGHVAYYGDGMLFSGDTLFGAGCGRLFEGTPGQMYSSLQQLAQLPVNTRVYCGHEYTERNLAFALSLEPHHEALLSRREATAALRAQGLPSLPSSMALELATNPFLRCHEPGIIAASKSAATDPVSVFAAIREMRNHF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must be able to hydroxyacylglutathione hydrolase activity.2. A protein that can modulate the overall flux of metabolites through the methylglyoxal degradation pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "SLIQWTLQHKASLTPHTLYATGTTGHLLARESGLNIQALLSGPMGGDQQLGGLIAEKKIDILIFFWDPMNAAPHDPDVKALMRIATVWNIPVAA",
    "response#2": "GVNVVDLYEAFKVFEENGFDIVIASDTGDYGFDDKSFRDPAIVDETQSIFSNPDCSLMKKLKNIARLDRLNPSDYVIVYIPGGYGCSFDFPHAKVVQDFLYRFYETKGIICAVAQANIALAYTTNSDGQALCTNRRVTGCTWKDEVQNGVLNVMNRLNFYSFGHIAENIGAIFESPPVYVEDPFIVEDGQLFTGSNTC",
    "response#3": "SLIQWTLQHKASLTPHTLYATGTTGHLLARESGLNIQALLSGPMGGDQQLGGLIAEKKIDILIFFWDPMNAAPHDPDVKALMRIATVWNIPVAA"
  },
  {
    "reference": "MGGKWSKRSIVGWPEIRERIRRTPPGAAAEGVGAASKDLEKCGAVTSSNTAHTNAACAWLEAQEDEEVGFPVRPQVPLRPMTFKGAFDLGFFLKEKGGLDGLIYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGVRYPLCFGWCFKLVPVDPREVEEANEGENNCLLHPMGQHGFDDEEKEVLMWKFDTSLARRHIARELHPEFYKD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that contributes to suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II by functioning in extracellular region, host cell Golgi membrane, membrane with GTP binding.2. The protein design should include a Diacidic; necessary for CD4 internalization, Dileucine internalization motif; necessary for CD4 internalization motif to ensure proper function. The designed protein sequence is ",
    "response#1": "GAPGCR",
    "response#2": "GSSGH",
    "response#3": "MICRG"
  },
  {
    "reference": "MEEVVVPRHGDHHNINIKYGFFIFALTIIQTIFFLQVKFIQIKRWNSTGRFSKFWSQITNPPIWLMVTVWLLIVIFTGGHKISDFSEEYIISAKRYGRMAYCLIPLNIYLVLRPTNSPLLKPGYYLENMSLHKWTSRIIVFCSTIHAAGYVYKWIKEGAILNKPFRFLNLLGVVVFVFLVVLAIISIRPFRRKVYSTFYLIHNVTAWSMVILITFHARPGVTVFAVISLILLGYQLYLRYYSSYMVNSLKVIDIPTSTLQIIKIPQSNKFPNWLPGSHIRLNYTISKFKSWTTASHPFTVVTIPEDSTNNLTLIVRKPNSFVINPLDSYLVTGPYPSLAPPFFTTANIVNIICGGSGISLGLPIYHHFKSINSTVPVKLVWTIRNQNDTFIMNQLDMTGVQVYVTSIGDTNSEQQENQQQAVPLFVIEEEEEEQGHGLLNNDNENGIELQNMPKTNEESSEANSTNSKNNKDNQERKEYFKFGRPKFDEVFAIDDPTTTYDLDNSWVIACGPDELISDAKRWSKDRGYRFYYEKYEM",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Probable cell surface metalloreductase; May be involved in iron or copper homeostasis ..2. The designed protein should contain one or more Ferric oxidoreductase, FAD-binding FR-type domains that are essential for its function.3. The protein can be expressed in membrane and perform oxidoreductase activity without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "LQPFLAPVVSNQRVTGPSHFQDVRLIDFDITGSGISFAAGDVVLIQPSNSAAHVQQFCQVLGLDPDRCFTLQPREAGVPCPPGLPQPCSVQHLVTQYLDIASVPRRSFFELLACLSAHELEREKLLEFSSAQGQEELHEYCSRPRRTVLEVLCDFPHTAGAIPPDYLLDLIPAIRPRAFSIASSLLAHPSRLQILVAVVQYQTRLREPRRGLCSSWLASLDPGRGAVRVPLWVRPGGLAFPG",
    "response#2": "LQPFLAPVVSNQRVTGPSHFQDVRLIDFDITGSGISFAAGDVVLIQPSNSAAHVQQFCQVLGLDPDRCFTLQPREAGVPCPPGLPQPCSVQHLVTQYLDIASVPRRSFFELLACLSAHELEREKLLEFSSAQGQEELHEYCSRPRRTVLEVLCDFPHTAGAIPPDYLLDLIPAIRPRAFSIASSLLAHPSRLQILVAVVQYQTRLREPRRGLCSSWLASLDPGRGAVRVPLWVRPGGLAFPS",
    "response#3": "LQQNNRITADNHWQDVRKVVFSIPKSVSWKPGDVAILFPWNDDESVHKFLRCLKWDSFADHLVQVTSNIPEKKAPWLPNPLTVFNFVKYVISIHSIPNRTFFEYAAFFTDNDMHKERLLEFASLEGLDDYYDYVTRPRRTLLETLQEFHSVQIPFEYALDALPIIRGRQYSIANRCNQIEGKLELIIALVKYQTILKEPRQGVCSRFIVTVKEIERTTCPSHFQDVRLVKLQTQGQHYVPGDVVVIRPKNLPSHITEFQNLLNCNGIDISPNEVFKITEINRDVPVPAPLQNAVTFQQLCEEYFDLNAVPRRHFFQILSLLTDNEIEKEKLQEFATAEGQNDLYNYCNRPRRNVVEVLQDFPHALKNVTKKVLFEIFHPIKPREFSIASSFKAHSNEIHILLGVVRYKTRLVKERLGLCSNR"
  },
  {
    "reference": "MSDATSAPAAVIVLAAGAGTRMKSKTPKIMHPIGGTSMVGHALAAANGLDPQHLVAVVRHQRDKVAAHLDELAEQHQWRLLVADQDEVPGTGRAVQAGLEALQQAEQSVGGTVVVTYGDVPLLTSELLRELVATHQQDGNAVTVLTALLEDPTGYGRILREADGSVAGIVEHKDATEEQRLINEVNSGIYAFDGATLVSALSQVTTDNAQGEMYLTDVLGLARAAGGRVAAVATQDRWQVEGANDRVQLQALGAEMNRRTLERHMRAGTTIVDPATTWIDSTVTLEQDTTILPNTHLQGTTHISEDAVIGPDTTLVDTHVGAGAKVTRTQAAGARIGEGAAVGPFAYIRPGTVLGEQGKIGAFYETKNVTIGRGSKLSHLGYAGDAEIGEFTNIGCGNITANYDGVNKHRTVIGDHVRTSSNTVFIAPVTVGDGAYTGAGAVVRKDVPAGSLALTVAPQRNAEGWTEQKRPGSPAAEAAARSRQAAENGQPDDGRS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.2. The protein design should be able to enhance the efficiency of regulation of cell shape. The designed protein sequence is ",
    "response#1": "IQGTAAVTVGSLLAACKAAGSSLAQQRVTFLGAGSAGCGIAEAIIAQMVSEGISDAQARSQVYMVDRWGLLQEGMPNLLDFQQRLVQKAENTKEWVSEEPNYSLFDVMHNAKPTVLIGVSGAPGLFSKEVIQEMHKHCERPIVFPLSNPTSRVEATPNDIIRWTDGQALVATGSPFDPVTHNGQTYPIAQCNNSFIFPGIGLGVLAIKATRVSDEMLRESSRALSECSPLAINGSGALLPPLEEIHTVSKKIAFAVAKKAI",
    "response#2": "IQGTAAVTVGSLLAACKAAGSSLAQQRVTFLGAGSAGCGIAEAIIAQMVSEGISDAQARSQVYMVDRWGLLQEGMPNLLDFQQRLVQKAENTKEWVSEEPNYSLFDVMHNAKPTVLIGVSGAPGLFSKEVIQEMHKHCERPIVFPLSNPTSRVEATPNDIIRWTDGQALVATGSPFDPVTHNGQTYPIAQCNNSFIFPGIGLGVLAIKATRVSDEMLRESSRALSECSPLAINGSGALLPPLEEIHTVSKKIAFAVAKKAR",
    "response#3": "IQGTAAVTVGSLLAACKAAGSSLAQQRVTFLGAGSAGCGIAEAIIAQMVSEGISDAQARSQVYMVDRWGLLQEGMPNLLDFQQRLVQKAENTKEWVSEEPNYSLFDVMHNAKPTVLIGVSGAPGLFSKEVIQEMHKHCERPIVFPLSNPTSRVEATPNDIIRWTDGQALVATGSPFDPVTHNGQTYPIAQCNNSFIFPGIGLGVLAIKATRVSDEMLRESSRALSECSPLAINGSGALLPPLEEIHTVSKKIAFAVAKKAI"
  },
  {
    "reference": "MAENFPKAILFSGTSHPKLAKGISDNLGIELGRVALDHFPDGEISVQIQENVRGRDVFIVQTVALDPNNYLMELLIMIDALKRASSQSITVVIPYFGYARQDRKDRPRVPITAKLVANLLQNAGASRLLTMDLHTDQLQGFFDIPVDNLYARPCIVEGVKRDLDPSNLVVVTPDIGSVKLAKAYAQQLGVDFAIVDKHRCDSSTIDVVTLIGDVKGKDVLLADDMCSTAGTLVSAAKVCREKGANRIFAAVTHGLFVGSAVEKIENSPIEVLYMSNTIPYTERLDGAEKIRTVSVANLFGQAIHCILSRESISSLFKLDCSAEPSLSRS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. For general function, the protein need meet that Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P).3. The ideal protein would be one that plays a critical role in ribonucleoside monophosphate biosynthetic process, nucleotide biosynthetic process, 5-phosphoribose 1-diphosphate biosynthetic process through magnesium ion binding, ribose phosphate diphosphokinase activity, ATP binding within cytoplasm.4. The designed protein should have a high specificity for ATP, Mg(2+) binding, with minimal off-target effects.5. The designed protein have Ribose-phosphate pyrophosphokinase N-terminal domains with specific sequence motifs that are crucial for its activity.6. Ideal protein should have a binding affinity for key enzymes in the Metabolic intermediate biosynthesis, 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1, 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MKLFAGNATPELAQKIADRLYIKLGEASVGRFSDGEISVAINENVRGSDVFILQSTCAPTNDNLMELIVMVDALRRASAGRITAVMPYFGYARQDRRVRSARVPITAKVVADFLSSVEHVQIDLHAAQIQGFFDCPVDHLFAAPLLANYFIQHNLTKDVVIVSPDHSSVARARQMGTLLGGAPIAIIDRRYSKAEQTENLEIIGDVKGKTAIIVDDMIDTGSRVTTSVEALKNGGAKDIYAAATHAVFSDHATQKLQASALKKVIVTDIILKDMFKLNLKNPIVVSPDIGGVIRARAIAKLFNDTDMAIIDKRRSTTNKLKIMNVIGDVTNRDCILVDDIIDTANTLCKAAQALKNRGSKRIFAYATHPVFSGNSKKNLIQSVIDEIIVCDAIPTTAACQQAAPVINEYLRNKGYSDDNIVVVSPDEGSIKRAIGHVKRLGGNLAIVDKRRASAMETTQQNLIGGPIEGKVALMFDDMISTAGSICGAAELVAAAGASEIHVAATHGVLCGPAIENLFVSATRIFG",
    "response#2": "MKLFAGNATPELAQKIADRLYIKLGEASVGRFSDGEISVAINENVRGSDVFILQSTCAPTNDNLMELIVMVDALRRASAGRITAVMPYFGYARQDRRVRSARVPITAKVVADFLSSVERLVDLHSAQIQGYFDIPFDHVFAEVEKLGLDKLVVCSPDVGGIRLARAYARSLNAGLAIVDKRRVGATETVSENVIGEVAGKNVLLVDDMISTAGSITEATRMLQSRGAERVYIAVTHAVLCGPAVERLENCPCESILVTNTIPLAKEREFAPKIKVLPIAPLLGEAIKRVHEGLSITKMFEEG",
    "response#3": "MKLFAGNATPELAQKIADRLYIKLGEASVGRFSDGEISVAINENVRGSDVFILQSTCAPTNDNLMELIVMVDALRRASAGRITAVMPYFGYARQDRRVRSARVPITAKVVADFLSSVENAMCSLDVDIAAPVINEYLRNKGYSDDNIVVVSPDEGSIKRAIGHVKRLGGNLAIVDKRRASAMETTQQNLIGGPIEGKVALMFDDMISTAGSICGAAELVAAAGASEIHVAATHGVLCGPAIEATPVLVKYFKSIREQLGEIALVSPDVGNVKMATVYASYLDGELAIIDKRRQSGSEVQSANLIGDVKDKTVLMFDDMITTAGTMCEAAKLVMDRGARRVMAASTHPVLVGLAMERLAEAPIDKIVVCDTSGSIACTPDALYSAEGKPMPKVYMHAAPVLNDHFLSRGLDEDEIVVVSPDEGSIKRAVGHAKRLGGPLGIIDKRRSSALEVRQSTIIGGPVEGRIALMFDDMISTAGSICGAARLVHDAGAKEIHIACTHGVLCGPAIHRLREAPIDSVVVTDGRLPYDAPVLLNYLQSKQLRDVVVVSPDIGGVGRARAFAKKFNDAPLAIVDKRRTAHNCAKVMNVIGDVKGKTAILVDDMIDTAGTITEAAKILRQEGARQVFACATHAVFSPPAIERLSSGCLEEVIVTNTTPIANTHAMPSVAPLFIDELATLFP"
  },
  {
    "reference": "MFKSLHQYAHVFSRLSLFGLAFAAAAQAQSQSLTVISFGGATKAAQEQAYFKPFERSGGGQVVAGEYNGEMAKVKAMVDVGKVSWDVVEVESPELLRGCDEGLFERLDPARFGDPAQFVPGTFSECGVATYVWSMVMAYDSTKLARAPQSWADFWNVREFPGKRGLRKGAKYTLEVALLADGVKAEDLYKVLATPEGVSRAFAKLDQLKPNIQWWEAGAQPPQWLAAGDVVMSAAYNGRIAAAQKEGVKLAIVWPGSLYDPEYWAVVKGTPNKALAEKFIAFASQPQTQKVFSEQIPYGPVHKGTLALLPKTVQEALPTAPANLEGARAVDAEFWVDHGEELEQRFNAWAAR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Binds specifically gamma-aminobutyric acid (GABA) with nanomolar affinity. The designed protein sequence is ",
    "response#1": "ILNKLLREYDKKLRPDIGIKPTVIDVDIYVNSIGPVSSINMEYQIDIFFAQTWTDSRLRFNSTMKILTLNSNMVGLIWIPDTIFRNSKTAEAHWITTPNQLLRIWNDGKILYTLRLTINAECQLQLHNFPMDAHACPLTFSSYGYPKEEMIYRWRKNSVEAADQKSWRLYQFDFMGLRNTTEIVTTSAGDYVVMTIYFELSRRR",
    "response#2": "TQILNSLLQGYDNKLRPDIGVRPTVIETDVYVNSIGPVDPINMEYTIDIIFAQTWFDSRLKFNSTMKVLMLNSNMVGKIWIPDTFFRNSRKSDAHWITTPNRLLRIWSDGRVLYTLRLTINAECYLQLHNFPMDEHSCPLEFSSYGYPKNEIEYKWKKPSVEVADPKYWRLYQFAFVGLRNSTEISHTISGDYIIMTIFFDLSRRR",
    "response#3": "TQILNSLLQGYDNKLRPDIGVRPTVIETDVYVNSIGPVDPINMEYTIDIIFAQTWFDSRLKFNSTMKVLMLNSNMVGKIWIPDTFFRNSRKSDAHWITTPNRLLRIWSDGRVLYTLRLTINAECYLQLHNFPMDEHSCPLEFSSYGYPKNEIEYKWKKPSVEVADPKYWRLYQFAFVGLRNSTEISHTISGDYIIMTIFFDLSRRR"
  },
  {
    "reference": "MKNSFLINILIIYYLFIILFVNSQDLKLGGSCELIDSNSPCFSKLNYTNFYLQPGDSITQLNKNVSDIIRMLEFTTPECKPNAINIMCLKSYPKCETYNETLSNNTNIIFNLPSLPCNSICLKAETPCKIFIDNFIKDLSCNSNFSNGAPMFPINSTDYEFKESGNFNFNVECNDNIIYDNSSSVINCPAPLLNSKDHVIPGKTTYYYITDSCILDCPFEIYPGKTKILDRTNYTLTSISFITCIFMILTFGVLPNKITHRMESILSFACGGCITALSLFIQSRQDNFNCSSDPGRFKSQSDYLCLLTGLIFQFGAITSIFWSPMIAYDFYITSTLGKIRKFGLYRIVLWSFIFVLTALPAFGGKYSATVATNCWINSDDGSAWQYVSFYIPSWCAMGLICLFSILSVINVSKMYIQTPNNRILFFNIKILITLLLFLFVLTFASSLKFYMEERMDTYFDAIAVWVECIGKGDPSQCELHAPGYDLKALNIVVIGILGFTVFIGYGLDPIVIHIWMESKKFQWVLKKCRLDKIIKLNNSINNSNNNNNETASTSSGNERKQTTVKMSNLKSTEINQQP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The Cytoplasmic, Extracellular topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.2. The protein should contain Helical; Name=3, Helical; Name=4, Helical; Name=5, Helical; Name=1, Helical; Name=2 transmembrane regions to facilitate transport across the membrane.3. The designed protein should contain one or more FZ domains that are essential for its function.4. The designed protein localizes to the membrane. The designed protein sequence is ",
    "response#1": "MFFSHHHTRFARLWILTWSVLCCASTFFTVTTSLVAMQRFRYPERPIIFLSGCYTMVSVAYIAGFVLQERVVCNERFSEDGYRTVGQGTKKEGCTILFMMLYFFSMASSIWWVILSLTWFLAAGMKWGHAAIEANSQYFHLAAWAVPAVKTITILAMGQIDGDLLSGVCFVGLNRLDPLRGFVLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEPLERLMVRIGVFSVLYTVPATIVIACYFYEQAFREHWERSWVSQHCKSLAIPCPAHYTPRTSPDFTVYMIKYLMTLIVGITSGFWIWSGKTLHSWRKFYTRLTNRRSG",
    "response#2": "MFFSHHHTRFARLWILTWSVLCCASTFFTVTTSLVAMQRFRYPERPIIFLSGCYTMVSVAYIAGFVLQERVVCNERFSEDGYRTVGQGTKKEGCTILFMMLYFFSMASSIWWVILSLTWFLAAGMKWGHAAIEANSQYFHLAAWAVPAVKTITILAMGQIDGDLLSGVCFVGLNRLDPLRGFVLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEPLERLMVRIGVFSVLYTVPATIVIACYFYEQAFREHWERSWVSQHCKSLAIPCPAHYTPRTSPDFTVYMIKYLMTLIVGITSGFWIWSGKTLHSWRKFYTRLTNGE",
    "response#3": "FFSHHHTRFARLWILTWSVLCCASTFFTVTTSLVAMQRFRYPERPIIFLSGCYTMVSVAYIAGFVLQERVVCNERFSEDGYRTVGQGTKKEGCTILFMMLYFFSMASSIWWVILSLTWFLAAGMKWGHAAIEANSQYFHLAAWAVPAVKTITILAMGQIDGDLLSGVCFVGLNRLDPLRGFVLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEPLERLMVRIGVFSVLYTVPATIVIACYFYEQAFREHWERSWVSQHCKSLAIPCPAHYTPRTSPDFTVYMIKYLMTLIVGITSGFWIWSGKTLHSWRKFYTGSARCQPVEIPMCQGIGYNLTRMPNLLGHESQREAGIKLHEFAPLVEYGCHAHLRFFLCSLYAPMCTDQVSASIPACRPMCEQARRACAPIMEGFSFGWPDSLDCARLPTHNDPHALCMEAPENAC"
  },
  {
    "reference": "MQTSFHKFFLSMILAYSCCSLSGGGYAAEIMIPQGIYDGETLTVSFPYTVIGDPSGTTVFSAGELTLKNLDNSIAALPLSCFGNLLGSFTVLGRGHSLTFENIRTSTNGAALSDSANSGLFTIEGFKELSFSNCNSLLAVLPAATTNNGSQTPTTTSTPSNGTIYSKTDLLLLNNEKFSFYSNLVSGDGGAIDAKSLTVQGISKLCVFQENTAQADGGACQVVTSFSAMANEAPIAFIANVAGVRGGGIAAVQDGQQGVSSSTSTEDPVVSFSRNTAVEFDGNVARVGGGIYSYGNVAFLNNGKTLFLNNVASPVYIAAEQPTNGQASNTSDNYGDGGAIFCKNGAQAAGSNNSGSVSFDGEGVVFFSSNVAAGKGGAIYAKKLSVANCGPVQFLGNIANDGGAIYLGESGELSLSADYGDIIFDGNLKRTAKENAADVNGVTVSSQAISMGSGGKITTLRAKAGHQILFNDPIEMANGNNQPAQSSEPLKINDGEGYTGDIVFANGNSTLYQNVTIEQGRIVLREKAKLSVNSLSQTGGSLYMEAGSTLDFVTPQPPQQPPAANQLITLSNLHLSLSSLLANNAVTNPPTNPPAQDSHPAIIGSTTAGSVTISGPIFFEDLDDTAYDRYDWLGSNQKIDVLKLQLGTQPSANAPSDLTLGNEMPKYGYQGSWKLAWDPNTANNGPYTLKATWTKTGYNPGPERVASLVPNSLWGSILDIRSAHSAIQASVDGRSYCRGLWVSGVSNFFYHDRDALGQGYRYISGGYS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The presence of Autotransporter domains is critical for the interaction of the designed protein with its intended targets.2. The protein must contain a signal peptide for proper functionality.3. The designed protein should be expressed specifically in the extracellular region The designed protein sequence is ",
    "response#1": "GVLSIEDRAGWQFYKFTQKGGILKLGHAASIATTANSETPSTSVGSQVIINNLAINLPSILAKGKAPTLWIRPLQSSAPFTEDNNPTITLSGPLTLLNEENRDPYDSIDLSEPLQNIHLLSLSDVTARHINTDNFHPESLNATEHYGYQGIWSPYWVETITTTNNASIETANTLYRALYANWTC",
    "response#2": "GVLAVEDGAGLACYKFFQRGGTLLLGQGAVITTAGTIPTPSSTPTTVGSTITLNHIAIDLPSILSFQAQAPKIWIYPTKTGSTYTEDSNPTITISGTLTLRNSNNEDPYDSLDLSHSLEKVPLLYIVDVAAQKINSSQLDLSTLNSGEHYGYQGIWSTYWVETTTITNPTSLLGANTKHKLLYANWSR",
    "response#3": "TSSMSMGWNFPLYEQVTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQISYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAK"
  },
  {
    "reference": "MTSQLEKEAREWIEETLHTKLNAQLDLLDQLQSGVILCRICKEALGANIRYKESNMPFVQMENISAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKLAEKKPRVFSAQQQREFREGVNSLQYGSFDMPTQGTEKIAFSRRRDPTGNMY",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Has actin-binding and actin-bundling activity and is a component of the actin patch; Cross-links F-actin and is required for the formation of the contractile F-actin ring.; Stabilizes actin filaments against disassembly.2. The ideal protein would be one that plays a critical role in actin cytoskeleton organization through cytoskeletal anchor activity within cytosol, actin cortical patch, actin filament, cell tip, nucleus.3. The protein contains novel Calponin-homology (CH) domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "GVSVADESLQAFNDLKLGKKYKFVLYGISEDKTTIVVKETSTSQSYDEFLGKLPENDCLYAIYDFEYEIGGNEGKRSKIVFFTWSPDTAPVRSKMVYASSKDALRRALTGVSSDIQGTDFSEVSFETVLERVR",
    "response#2": "DESLQAFNDLKLGKKYKFVLYGISEDKTTIVVKETSTSQSYDEFLGKLPENDCLYAIYDFEYEIGGNEGKRSKIVFFTWSPDTAPVRSKMVYASSKDALRRALTGVSSDIQGTDFSEVSFETVLERVD",
    "response#3": "GVSVADESLQAFNDLKLGKKYKFVLYGISEDKTTIVVKETSTSQSYDEFLGKLPENDCLYAIYDFEYEIGGNEGKRSKIVFFTWSPDTAPVRSKMVYASSKDALRRALTGVSSDIQGTDFSEVSFETVLERVI"
  },
  {
    "reference": "MVCVCNATYCDTLDPLALPAPGTYVKYESSKAGKRLERSEGSFQHSLHAPGLLLTLNVSALYQRMKGFGGSLSDSAAINILELSRPAQDQLLRSYFSESGIEYNLLRLSMACSDFSTRPYSYDDVPHDYELKHFRLAEEDLMMKIPLLHRASAISKRPLSLIGSPWTAPAWLKSNNDVRGKGTLKGQAGDKYHKTWANYFIKFLDEYSKHNVTFWAVTAQNEPLAALLTPPQFPTIAFTAASQRDFVVHDLGPALARSAHPTRLIIMDDQRIHLPHWAKVVLGNATAARYATGVGVHWYLDSIAPASCLDATHKLFPDHLLLYTEACSGILTLRFSVSLGCWDRGVHYSHSILTVLNHFVAGWTDWNLALNLKGGPNWVKNYVDSPVIVDSSKDVFYKQPMFYHLGHFRWVRGAPGASCQLKIRGCDPCPCSSPHSKFIPEGSQRVGLHSSRRCLLCQLEHVAVLRPDGALVLVVLNK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein have enhanced cholesteryl 3-beta-D-glucoside + H2O = cholesterol + D-   glucose.2. A protein should be able to manipulate the activity of enzymes in the cholesterol metabolism pathway, either by activating or inhibiting their function.3. The presence of Glycosyl hydrolase family 30 TIM-barrel, Glycosyl hydrolase family 30 beta sandwich domains is critical for the interaction of the designed protein with its intended targets.4. The designed protein should have glycosyltransferase activity, glucosylceramidase activity within lysosomal membrane to regulate sphingolipid metabolic process. The designed protein sequence is ",
    "response#1": "MPMASCDFSVRPYTYDDFPYDYELKNFSLVGEDLLMKIPIVHRAKAMSKRPISLMASPWTAPIWMKSNEAWKGKGWLKGEAGDSIHKTWASYFVRFLDEYAKQNLTFWAITAQNEPSSGFVKDYPFQTLGFTAESQREFIASDLGPALAKSSHKNIRLITLDDNRLHLPKWAKEVLSTESQAYRYIHGIGLHWYMDFISPIKGTVEATQRLFPDYFLIATEACAGSFFWEQDVILGSWDRGEDYSHSILENLNHHVVGWVDWNLALDLQGGPNWVKNYVDSPIIVDASSDIFYKQPMFYHLGHFSKFIPEGSQRVGLVASKESKKVDLEYTAFLRPDGAVVVVVLNR",
    "response#2": "MPMASCDFSVRPYTYDDFPYDYELKNFSLVGEDLLMKIPIVHRAKAMSKRPISLMASPWTAPIWMKSNEAWKGKGWLKGEAGDSIHKTWASYFVRFLDEYAKQNLTFWAITAQNEPSSGFVKDYPFQTLGFTAESQREFIASDLGPALAKSSHKNIRLITLDDNRLHLPKWAKEVLSTESQAYRYIHGIGLHWYMDFISPIKGTVEATQRLFPDYFLIATEACAGSFFWEQDVILGSWDRGEDYSHSILENLNHHVVGWVDWNLALDLQGGPNWVKNYVDSPIIVDASSDIFYKQPMFYHLGHFSKFIPP",
    "response#3": "MPMASCDFSVRPYTYDDFPYDYELKNFSLVGEDLLMKIPIVHRAKAMSKRPISLMASPWTAPIWMKSNEAWKGKGWLKGEAGDSIHKTWASYFVRFLDEYAKQNLTFWAITAQNEPSSGFVKDYPFQTLGFTAESQREFIASDLGPALAKSSHKNIRLITLDDNRLHLPKWAKEVLSTESQAYRYIHGIGLHWYMDFISPIKGTVEATQRLFPDYFLIATEACAGSFFWEQDVILGSWDRGEDYSHSILENLNHHVVGWVDWNLALDLQGGPNWVKNYVDSPIIVDASSDIFYKQPMFYHLGHFSKFL"
  },
  {
    "reference": "MSITKYIFVTGGVTSSLGKGIISASLAKLLQSRGLSVTIQKFDPYLNIDPGTLNPYEHGECYVTDDGAETDLDLGHYERFLNIATSQANNITTGRIYNNVITRERRGDYLGKTVQVIPHITDEIKRNFFLLGNTGQYDIVITEIGGCVGDIESLPFIEAVRQARWELGEHNALVIHLTLVPYLKSAGELKTKPTQHSVKTLLEAGIQPDILVCRTEHHLPQEIRRKLALFCNVHINSVIESIDAETIYDVPLLMKKEKLDERVISKMRLTPKQETNIESWRDFLGRLKNPTEEVNIALVGKYVELHDAYKSIAEAFIHAGAANECKVNLHWIKAEDLVSWETTVSTLSKVDGILVAPGFGERGIEGKINAVRYARENNVPFFGICLGMQCAVVEFSRNVLGLKDAASTEMNPDTPNPVISIMESQKDVTMKGGTMRLGAYRCELQKGSKAYQAYGKAEISERHRHRYEFNSDYRDQIEAAGMIASGINPDTGLVEIIELKDHVWFVGAQYHPELKSTVLNPHPLFVKFVRVALETYKAKHLAEHR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates..2. The protein should be optimized for activity in CTP biosynthesis via de novo pathway pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The designed protein must have a active site that is structurally stable and resistant to denaturation.4. The designed protein must be optimized for metal ion binding, CTP synthase activity. The designed protein sequence is ",
    "response#1": "VEIAIVGKYTQLHDAYLSVVEALKHGGISCHADIQFRWTDSEEITEENLEEKLGGVDGILVPGGFGKRGTEGMILAADYARRENIPYLGICLGMQMAIVAFARYVVGYHDADSIEFHPETLHPLIALMPDQKDVENIGGTLRLGAYPCVLDEHSRSCALYGKTEISERHRHRYEVNNDYREVLEKAGLHLAGLSPDKRIVEMIEITDHPFYIGTQAHPEFKSRPNRPHPLFAGFVTIGMVGKYTELPDAYKSINEALKHGGLKNQLTVNIKYIDSEDVEVRGTELFEGLDAILVPGGFGVRGVEGKIIAAQYARENNIPYLGICLGMQVAIIEYARHVAGLEGANSSEFDAKTKYPVIALITEWMDEDGNVEVRTDKSDMGGTMRLGGQLCHLKKDSLVRSLYGKDDIVERHRHRYEVNNNLLSKITDAGLNVAGLSTDNKLVEIVEIPEHRWFIGVQFHPEFTSNPREGHPLFSGFF",
    "response#2": "LQDAYKSLREALVHGGIANKVKVNIEWIDSEIFESEDAVLHLEGVNGILVPGAFGKRGAEGKIKSITFARERLVPYFGICFGMQMAVVESARNLAGLPDASSTEFEKPCLPLVGLMTAWEHDGGQVTRSASSDMGGTLRLGAYDAHLTEGSQVQKIYGATTISERHRHRYEVNINYVEKLEETGMVFSGMSPDGVLPEIVEIPEHPWFIGVQFHPELKSRPLDPHPLFVSFIR",
    "response#3": "LQDSYKSILESFIHAGAENEVKVNIRSIHSEFIDIEGKTKITDLDGILVAPGFGERGIEGKIEAVRFARENNLPFFGICLGMQMAVIEYARNVLNLPDANSTEMEAQTKNPVIDIMESQKNITQMGGTMRLGAWECELKEGTKVFEAYGKSTISERHRHRYEYNNKYKEQLEAAGMLSSGTNPQTGLVEVVEIPTHPWFIGVQYHPEYKSTVKTPHPLFIGFVR"
  },
  {
    "reference": "MLDPLKTSSYDYNLPKNQIATYPVTPADSAKLLIFNRSTNTIIHSTFKDILEFLPNDLSIFLNDTKVIKARIFGVKDSGGQIELLLNKPLFMDRYLVMIRGKVRVGTKLLFDENLSAIVEEVDEDGSRIVEFFQDEKKLDFLSLVEILNKIGHLPLPPYMNREDEKQDEQNYQTLFAKNYGAVAAPTASLHFTPELLKNLEEKYGLNYLTLHVGAGTFKPVDVEDILSHPMHSEYFEIGIDAKKNLDKANKVLAVGTTVTRTIEYYARTNKIQGECDLFLNPANKPIKVDYLLTNFHLPKSTLIMLVASFIGLEKTLEIYETAIKENYRFYSYGDGMLII",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in queuosine biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.2. For general function, the protein need meet that Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA). The designed protein sequence is ",
    "response#1": "RGRACSFHTPHGIVETPRFMPVGTLATVKTVTPAQLRDTGAQMVLSNTYHLHLQPGEEIVAKAGGLHRFMNWSGPMLTDSGGFQVFSLSELRKIEERGVTFRSPRDGAVIEFTPERSIRIQNALGADVIMAFDECPPYPAERKDVEAAVARTYRWLERCINAHERPQDQALFGIVQGGVYLDLRQQAARDLVQLDLPGYAIGGVSVGEPSEEIHRIVEATAPLLPAHKPRYLMGVGTYREMVQAIAAGIDLFDCVIPTRLARHGAALVKGDRWNLKNAQFREDFQPLDEDCNCYCCQNFSRAYLNHLIRSREILGYTLLSIHNITELVRFTTRIREAILSDRFVEEFL",
    "response#2": "RGRACSFHTPHGIVETPRFMPVGTLATVKTVTPAQLRDTGAQMVLSNTYHLHLQPGEEIVAKAGGLHRFMNWSGPMLTDSGGFQVFSLSELRKIEERGVTFRSPRDGAVIEFTPERSIRIQNALGADVIMAFDECPPYPAERKDVEAAVARTYRWLERCINAHERPQDQALFGIVQGGVYLDLRQQAARDLVQLDLPGYAIGGVSVGEPSEEIHRIVEATAPLLPAHKPRYLMGVGTYREMVQAIAAGIDLFDCVIPTRLARHGAALVKGDRWNLKNAQFREDFQPLDEDCNCYCCQNFSRAYLNHLIRSREILGYTLLSIHNITELVRFTTRIREAILSDRFVEEFL",
    "response#3": "RGRACSFHTPHGIVETPRFMPVGTLATVKTVTPAQLRDTGAQMVLSNTYHLHLQPGEEIVAKAGGLHRFMNWSGPMLTDSGGFQVFSLSELRKIEERGVTFRSPRDGAVIEFTPERSIRIQNALGADVIMAFDECPPYPAERKDVEAAVARTYRWLERCINAHERPQDQALFGIVQGGVYLDLRQQAARDLVQLDLPGYAIGGVSVGEPSEEIHRIVEATAPLLPAHKPRYLMGVGTYREMVQAIAAGIDLFDCVIPTRLARHGAALVKGDRWNLKNAQFREDFQPLDEDCNCYCCQNFSRAYLNHLIRSREILGYTLLSIHNITELVRFTTRIREAILSDRFVEEFA"
  },
  {
    "reference": "MSDLSDLTTEAASRDAADPLAHLRDRFDLDPDVVYLDGNSLGALPRGVAERVAEVITKEWGRDLIRSWFGNGWWTAPERVGDKIAPIIGAAPGQVVVGDSTSVNLFKALVGAARLALPDDGPPPGGAEASTGGGASNARTELLVDDETFPTDGYIASSAAELTGLVLRRVPAAGLAAAIGPQTAVVLANQVDYRSGTLLDLAELARAARAAGAVVVADLCHSAGALPVGVDALGIELAVGCSYKYLNGGPGAPAYLYVRADLQSRFRQPLSGWHGHVEPFAMRTEFERAGGIVAGRVGTPEILSLVALDTALDAWAGVEIEAVRAKSIALTDFFLRCVDELVPAGAVACVTPVEGARRGSQIALACADAERVMAELTERGIIGDFRPPDLLRFGFAPLYLRYADALRAATVLAEVLG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively.2. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis, NAD(+) biosynthesis pathway to avoid interference with native metabolic processes.3. The pyridoxal 5'-phosphate binding site should be located in a region of the protein that is accessible to the ligand.4. pyridoxal 5'-phosphate is required for the proper folding and stability of the protein.5. The protein contains novel Aminotransferase class V domains that confer a unique function or activity.6. The protein have enhanced H2O + L-kynurenine = anthranilate + H(+) + L-alanine.7. The designed protein should be involved in tryptophan catabolic process, 'de novo' NAD biosynthetic process from tryptophan, quinolinate biosynthetic process by localizing to cytoplasm and exhibiting kynureninase activity, pyridoxal phosphate binding. The designed protein sequence is ",
    "response#1": "MGNSPLTQWQDMAEQCAKQSADIVGASPHEIVIMNTLTINLHLMMASFYKPSGKRHKIILEWKPFPSDHYAIESQVVWHGLDPKTSMIKIEPDANSVIPTESILRTIDEHADETALLMLPGIQYYSGQLFDMPRITAYAKQRGITVGWDLAHAAGNVELKLHEWDVDFACWCTYKYINAGPGAIGGVYVHERFGKPLMPWAIKEQTAKILGALPTEVVIMETLTANLHLLMSSFYRPTKDRWKIIIEGKAFPSDHYAVLSQIANHSLDPSALITIDPPSASSPYLSNEHILSVISRHAATTALVLLPGIQFYSGQFFDIELITRHCQSLGITVGWDLAHAAGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHGQLLNPIQNPSYGEITLMNGCTVNLHFLMIHFYQPRVGRYKIICEENPFSSDLYCFKSQIRFHKLDPDKALIQVKPRQGERFLRNEDVIDAIEKHGKQTALVCFGGIQFFTGQRFDLEGVTKAAHAQGAVCGFDLAHMIGNCEMHLHRWNVDFAVWCSYKYLCGGQGAIGGALA",
    "response#2": "MGNSPLTQWQDMAEQCAKQSADIVGASPHEIVIMNTLTINLHLMMASFYKPSGKRHKIILEWKPFPSDHYAIESQVVWHGLDPKTSMIKIEPDANSVIPTESILRTIDEHADETALLMLPGIQYYSGQLFDMPRITAYAKQRGITVGWDLAHAAGNVELKLHEWDVDFACWCTYKYINAGPGAIGGVYVHERFGKQDPKAPVGEITLMNGCTVNLHFLMIHFYQPRVGRYKIICEENPFSSDLYCFKSQIRFHKLDPDKALIQVKPRQGERFLRNEDVIDAIEKHGKQTALVCFGGIQFFTGQRFDLEGVTKAAHAQGAVCGFDLAHMIGNCEMHLHRWNVDFAVWCSYKYLCGGQGAIGGAY",
    "response#3": "MGNSPLTQWQDMAEQCAKQSADIVGASPHEIVIMNTLTINLHLMMASFYKPSGKRHKIILEWKPFPSDHYAIESQVVWHGLDPKTSMIKIEPDANSVIPTESILRTIDEHADETALLMLPGIQYYSGQLFDMPRITAYAKQRGITVGWDLAHAAGNVELKLHEWDVDFACWCTYKYINAGPGAIGGVYVHERFGKPDEVVEDYSRKGEITLMNGCTVNLHFLMIHFYQPRVGRYKIICEENPFSSDLYCFKSQIRFHKLDPDKALIQVKPRQGERFLRNEDVIDAIEKHGKQTALVCFGGIQFFTGQRFDLEGVTKAAHAQGAVCGFDLAHMIGNCEMHLHRWNVDFAVWCSYKYLCGGQGAIGGAPPTKAPIAIKEQTAKILGALPTEVVIMETLTANLHLLMSSFYRPTKDRWKIIIEGKAFPSDHYAVLSQIANHSLDPSALITIDPPSASSPYLSNEHILSVISRHAATTALVLLPGIQFYSGQFFDIELITRHCQSLGITVGWDLAHAAGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHPPYPWSIDAIKEQTAKILGALPTEVVIMETLTANLHLLMSSFYRPTKDRWKIIIEGKAFPSDHYAVLSQIANHSLDPSALITIDPPSASSPYLSNEHILSVISRHAATTALVLLPGIQFYSGQFFDIELITRHCQSLGITVGWDLAHAAGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVH"
  },
  {
    "reference": "MSDKPNLEEVASFDKTKLKKTETQEKNPLPTKETIEQEKQAS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have actin monomer binding within cytoplasm, cytoskeleton to regulate actin filament organization. The designed protein sequence is ",
    "response#1": "QQKKTFTAWCNSHLRKAGTSIENIEDDFRNGLKLMLLLEVISGETLPKPDRGKMRFHKIANVNKALDFIASKGVKLVSIGAEEIVDGNLKMTLGMIWTIILRFR",
    "response#2": "LEFAKRAAPFNNWLDGAREDLVDMFIVHTMEEIQGLIQAHDQFKATLGEADKEFNVIIGLVRDAEAIVKQEQVPGGLVNPYTTLSADLISRKWSEVRALVPQRDQTLG",
    "response#3": "TDDIIVNWVNGTLSEAGKSTSIQSFKDKTISSSLAVVDLIDAIQPGCINYDLVKTGNLTEEDKHNNAKYAVSMARRIGARVYALPEDLVEVKPKMVMTVFACLMGR"
  },
  {
    "reference": "MGSKLSKSSFPGWDSIRERMRRTEPRAEPAADGVGAASRDLEMHGAITSSNTAANNAACAWLEAQEEEEVGFPVKPQVPLRPMTYKGAVDLSHFLKEKGGLDGLIYSRKRQEILDLWIYHTQGYFPDWQNYTPGPGIRYPLCFGWCFKLVPVEPDNEGENNCLLHPMSLHGAEDQEGEVLVWKFDSKLAFHHVARELHPEYYKDC",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that localizes to virion component, host cell plasma membrane, host cell Golgi membrane and can perform SH3 domain binding, GTP binding with high specificity and efficiency.2. Incorporating a Dileucine internalization motif; necessary for CD4 internalization motif is critical for the successful design of the protein.3. For general function, the protein need meet that Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD. The designed protein sequence is ",
    "response#1": "MEPVDPSLEPWKHPGSQPKTACTPCYCKKCCFHCQVCFITKGLGISYGRKKRRQRRRAPQDSQSHQVSLSKQPASQPRGDPTGPKESKKKVERKTETDP",
    "response#2": "MGQXKGWPXVRERMRRAEPAAEGVGAASRDLEKHGAITSSNTAATNADVAWLEAQQEEEEVGFPVKPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLCFGWCFKLVPVDPEKVEEANEGENNCLLHPMSQHGMDDPEREVLVWKFDSRLAFHHMARELHPEYYKDC",
    "response#3": "LRTLGGXQXR"
  },
  {
    "reference": "MSLLWTPQILTISFVSYILSLFPSPFPSCYTSCWFETSITTEKELNQYFELAKFLA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that May be associated with TAP2 isoform activity. The designed protein sequence is ",
    "response#1": "MVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTY",
    "response#2": "PEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTII",
    "response#3": "DFGFVESYNAVVRVAVDHEKKYLYIYWEYYKNGLTDDKTAEELKEFAETKELIKADSAEPKTIRYFQQHGFNMVGARKYQGSRLQYTKKIKRFRKIICSDRCENTIYELKPLAYATDKLGNIIEDEFTIDPHTLSAIWYALDR"
  },
  {
    "reference": "MRMGKIAVGYGFLLLLVFQLGVRASTLFNKYNVQELSSLKDLLERLEEKLSPGEESDVYAGSEDLVNDPEEDADLILDNVRKQAEKEFYKPAGFRDENLQRGRLRSIATSARSMNGCFGNRIERIGSWSSLGCNNSRFGSKKRIF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Exhibits natriuretic and vasodepressor activity.2. Incorporate a signal peptide in the protein design.3. The protein can be expressed in extracellular region and perform hormone activity without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "RMPSLSIDMPMSVLRQKLSLENERKLQSLRAMANRNFLNDR",
    "response#2": "HVCHLANATISAEKDHCPVCITFTTSICTGYCQTMDPVYKTALSSFKQNICTYKEIRYDTIKLPDCLPGTDPFFTYPVALSCYCDLCKMDYSDCTVESSEPDVC",
    "response#3": "RMPSLSIDMPMSVLRQKLSLENERKLQSLRAMANRNFLNDL"
  },
  {
    "reference": "MAGPMTFFSRRTSSIFLTSLPNLKAVFGVPAIILSLDICLDKEDLGNLWNILLDIFGVFKVSVRLLKSLPKAERFVPKSGIYPKGFVVGGIHTGVKKDTHALDLAMIHNVSGNDGIASAVFTTNRFKAAPVQVSSKIIKQKNGHVNSLVINSGNANAVTGTQGLQDAHNMIKVTDAVLENEEDSSLVMSTGVIGQKLPIDNILQGIPKLALSNLGSSHEHWLACAQAICTTDTFPKLVSKQFDLNGTTYTLAGLAKGAGMICPNMATLLGFFATDAPVTSNALNQILKYAVDRSFNSISVDGDMSTNDTIVAIANGAAGGKVIDASAESAEEFSVLRTEITGFAQQLAQLVVRDGEGATKFITIKVTDATSYREAKEISSSIANSSLFKTAMYGNDANWGRILCAIGYAKVNANAVNPSRTSVSLVPADGNEPLPLLVNGEPEDVDEERALQILQNEEIGIHVSLGTGGGQEATYWTCDLTHDYVTINADYSNVDKTHDIEQFWMRFMTSPLVNKPGMITAKRSTVMSQLQASHTVDFHSSDSGPIKIENSDSDYDSQVKLQDALEQASQYGQPSKDHNSMASAQAGRIAPNTLHSFHALKPQPSQSSSASQQQSARQTVPSAQFLTQVSGFPTQEHSSATSEESYPFSFMTEFSDATKNSYCLAALNEVPKKTNNKIYHTEAVVLGTVPEISRVVVKACSISNGNLTLSDPQISSLELIFVIF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should consider the endogenous proteins and enzymes present in the N(2)-acetyl- L-ornithine from L-glutamate: step 1/4, Amino-acid biosynthesis, L-arginine biosynthesis pathway to avoid interference with native metabolic processes.2. The protein must exhibit the following characteristics: Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate..3. The designed protein should have a high specificity for substrate binding, with minimal off-target effects.4. The protein with the reaction acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate, the Nucleophile must be optimized to ensure efficient catalysis.5. The protein should be optimized for mitochondrial matrix-specific folding and stability, while maintaining acetyl-CoA:L-glutamate N-acetyltransferase activity, glutamate N-acetyltransferase activity. The designed protein sequence is ",
    "response#1": "MTRAVVKYGGAAMVRADLKDRFAEDVRLLQAVGLRPIIVHGGGPEISRTLEQMGQTSEFIDGLRVTDAASLRVVEMVLTGQINKEVVSSLARAGAKAVGLSGKDGGLIEARKLQAPSGRDLGYVGEVAGVNPEVIELLVGKGFIPVVSPIGLGRDGHTYNINADTVAAELAVACGARKLIFLTDVAGILAKGLLVSEMSAEELELRMNDGTVTGGMLPKAQAILRALHGGVETVHIIRLTLPLEHQLLRGLGTETLISRGPSE",
    "response#2": "MKRAVVKYGGAAMVRADLKDRFAEDVRLLQAVGLRPIIVHGGGPEISRTLEQMGQTSEFIDGLRVTDAASLRVVEMVLTGQINKEVVSSLARAGAKAVGLSGKDGGLIEARKLQAPSGRDLGYVGEVAGVNPEVIELLVGKGFIPVVSPIGLGRDGHTYNINADTVAAELAVACGARKLIFLTDVAGILAKGLLVSEMSAEELELRMNDGTVTGGMLPKAQAILRALHGGVETVHIIELEEGKLGNLPVRVLRSMEEVDQDKLTHLLETSFKRTLDREGFYNRLKNDLDFVIVIGDYAGAAVCTLEGKPVSDSFAYPPNHPEPICYLDKFAVHPSHQGDGTVDFLWVALRDETYGLGQLDASNPSIGSLRGVGRGRDLVWRSRSDNPVNKWYYERSSGFLKTRDEKWKVFWCDAEQRLGEIWREREFGGGRLVRRLLEL",
    "response#3": "MLPVRVLRSMEEVDQDKLTHLLETSFKRTLDREGFYNRLKNDLDFVIVIGDYAGAAVCTLEGKPVSDSFAYPPNHPEPICYLDKFAVHPSHQGDGTVDFLWVALRDETYGLGQLDASNPSIGSLRGVGRGRDLVWRSRSDNPVNKWYYERSSGFLKTRDEKWKVFWCDAEQRLGEIWREREFGGGRLVRLAQLHSRVLRSMEEVDQDKLTHLLETSFKRTLDREGFYNRLKNDLDFVIVIGDYAGAAVCTLEGKPVSDSFAYPPNHPEPICYLDKFAVHPSHQGDGTVDFLWVALRDETYGLGQLDASNPSIGSLRGVGRGRDLVWRSRSDNPVNKWYYERSSGFLKTRDEKWKVFWCDALPVRVLRSMEEVDQDKLTHLLETSFKRTLDREGFYNRLKNDLDFVIVIGDYAGAAVCTLEGKPVSDSFAYPPNHPEPICYLDKFAVHPSHQGDGTVDFLWVALRDETYGLGQLDASNPSIGSLRGVGRGRDLVWRSRSDNPVNKWYYERSSGFLKTRDEKWKVFWCDAEQRLGEIWREREFGGGRLVRLAQLHSLPVRVLRSMEEVDQDKLTHLLETSFKRTLDREGFYNRLKNDLDFVIVIGDYAGAAVCTLEGKPVSDSFAYPPNHPEPICYLDKFAVHPSHQGDGTVDFLWVALRDETYGLGQLDASNPSIGSLRGVGRGRDLVWRSRSDNPVNKWYYERSSGFLKTRDEKWKVFWCDAEQRLGEIWREREFGGGRLVR"
  },
  {
    "reference": "MIVGLRGTIIRLEPMYAEIDVGGVIYGMQMSLNATSMLQTKIDSQPDAPVKIICAQIIREDAHLLFGFCEEIEKQTFERLIKISGVGPKVAIAILSTYTPTHFAKIIADKDIEALKKVPGIGVKGAAKIMVDIAGFFAQLLQSQEESIAPSNNLKYEASLALQSLGFKRNEIQKVLEHIEALSVSEIVKEALKRLA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a specific function in SOS response, DNA repair, DNA recombination and should be localized to Holliday junction helicase complex in order to carry out this function efficiently.2. The protein must exhibit the following characteristics: The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex. The designed protein sequence is ",
    "response#1": "LRPHVFADFTGQEKIVANLKIFVQAAKERKEPLDHVLLHGPPGLGKTTLAHIIANELGANIRITSGPVLDKPGDLAGLLTNLGPYDVLFIDEIHRLNPIVEEYLYTAMEDFKIDIMLDSGPSARSVQLNLNPFTLIGATTRSGLLTSPLRARFGINTRL",
    "response#2": "LRPKRLAEYVGQAKIREQLEIFIHAAKKRSEALDHVLLFGPPGLGKTTLAHIVAAEMGVNLRQTSGPVLERAGDLAALLTNLEPHDVLFIDEIHRLSPVVEEILYPALEDFQIDIMIGEGPAARSVKLDLPPFTLVGATTRAGMLTNPLRDRFGIVSRI",
    "response#3": "IRPSSFENFIGQKKLVETLQILISSSQKRKQSLDHILFYGPPGTGKTTLANIVANVLEAKIKYVQGPLLEKKSDVLAVLANISPDTIIFIDEIHGINKNIEELLYSAMEEFVIDLQIGVDGERKIMRMKLPQFTLIGASTKLAQISTPLQNRFGYVAT"
  },
  {
    "reference": "MENTRSENEEQPESTLKIDEEQPAVEQSPENQCSEEDQSSEDLSSEEQSSEEEFFPEELLPELLPEMLLSEDRPPQECLSQKNQFEDRIPMEQPPCGVGKHKLEEGSFKERLARIRPQFIGDIHGRNLSNEEMIQAADELEEMKRVRNKLMIMHWKAKRSRPYPI",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a specific function within the nucleoplasm.2. For general function, the protein need meet that May be involved in transcriptional regulation; Modulates various viral and cellular promoters in a promoter context-dependent manner3. The protein design should emphasize Acidic residues, Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "GQMLFPPMLLGVPVSFLEGSGDYLLCITSIGQMYCWNVNTGKIAFPINDVYSLLNPMLRYSDDVLSRAENITMCAVTSQGIPIVTLSNGDGYMFDSAMEAWMLINDSWWPYGSQYWNFMSSAGVDLTSNDDEKKDKYWNAEADILAKEVKNNKNSIINYLETKTNDELTRKGRMKHLQRFAKVLLMKEGFENLEEMITLAHLENKILVSFRLKEVEV",
    "response#2": "PDYEVLSNEPHETMPISAFAQKQRPPRFQNFLTPPRGSYTGSTQSILMDNDPLFSQSPWRSKLTGTDGETLGGFPVKFLVQVTRLSKILMIKKEHIKHLKEMNTEAEKLKSYSMPIGLDLQKRYATTVLDLEQLNKDLNKVLHEVQQFCFELAPDQGMQPADQPSELRRRCEEESQDVLRQNNTLASGEPRVQNTELTQLISRLTALLLQIRCLAEGGDLNSFEFKSLTDSLNDIKSSIDDSNLSCFQDNVEIHVAHIQSGLSQLGNLHAFSANNC",
    "response#3": "GQMLFPPMLLGVPVSFLEGSGDYLLCITSIGQMYCWNVNTGKIAFPINDVYSLLNPMLRYSDDVLSRAENITMCAVTSQGIPIVTLSNGDGYMFDSAMEAWMLINDSWWPYGSQYWNFMSSAGVDLTSNDDEKKDKYWNAEADILAKEVKNNKNSIINYLETKTNDELTRKGRMKHLQRFAKVLLMKEGFENLEEMITLAHLENKILVSFRLKEVEC"
  },
  {
    "reference": "MKEKRLIIKRSGYPSKGEIVIGTVKRVLDFGAFVSLDEYEGREGMVHISEVASGWIKDIREHVKKGQKVICKVLDVNPKRGHIDLSIKDVNERQRREKLQQWKNEMKAFKWLEIIGEKLNIDFKELEKIGKKLMKEYDSVYSAFEEAAFEGYEVLAPIVGEEFAKEMAEIARENIKPKRVKVRGYFELKSSASDGIERIKKALLEAKKALVNGVEMKLEYVGAPKYRIVVEADDYKTAENVLKKATENVLKAIKKLGGEGNFIREAA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. The designed protein sequence is ",
    "response#1": "KSFFKADRVAMLWNSKATAVLVTASTDVDKTGASYYGEQTLHYIAVNGESAVVQLPKNGPIYDVTWNKNATEFCVVYGFMPAKATVFNLKCDPIFDFGTGPRNAAFYSPQGHILVLAGFGNLRGQMEVWDVKKYKLISKPTASDATFFSWCPNGEHIITATCSPRLRVGNGYKIWHYTGTLLHKYDVPANSELWQC",
    "response#2": "KPKFVMRPPQVLRVGTKKTSFANFMDIAKTLHRLPKHLLDFLLAELGTSGSMDGNQQLIIKGRFQPKQIENVLRRYIKEYVTCHTCRSPETILQKDTRLFFLQCESCGSS",
    "response#3": "KSFFKADRVAMLWNSKATAVLVTASTDVDKTGASYYGEQTLHYIAVNGESAVVQLPKNGPIYDVTWNKNATEFCVVYGFMPAKATVFNLKCDPIFDFGTGPRNAAFYSPQGHILVLAGFGNLRGQMEVWDVKKYKLISKPTASDATFFSWCPNGEHIITATCSPRLRVGNGYKIWHYTGTLLHKYDVPANSELWQR"
  },
  {
    "reference": "MTELDAQKIIDFIANSKKQTAVKVTYKGHLNGRIPTSIQNFSNSDFGILYGDWSDIKPLLSGLNKKDYLIENNARNSAVPLLNIKDINARIEPGAIIRDQVKIADNAVIMMGAVINIGAEIGEATMIDMGAVLGGRAIVGKHSHIGAGAVLAGVVEPASAQPVRVGDNVLIGANAVIIEGVQIGDGAVVGAGAVVINDVPAHTVVAGVPAKVIKQIDEKTENKTALIDALRSL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be optimized for tetrahydrodipicolinate N-acetyltransferase activity activity in order to improve lysine biosynthetic process via diaminopimelate, diaminopimelate biosynthetic process.2. Ideal protein should have a binding affinity for key enzymes in the Amino-acid biosynthesis, LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (acetylase route): step 1/3, L-lysine biosynthesis via DAP pathway pathway to enhance the overall efficiency of the pathway.3. The desired protein should be able to perform (S)-2,3,4,5-tetrahydrodipicolinate + acetyl-CoA + H2O = CoA +   L-2-acetamido-6-oxoheptanedioate4. The protein must exhibit the following characteristics: Catalyzes the transfer of an acetyl group from acetyl-CoA to tetrahydrodipicolinate.. The designed protein sequence is ",
    "response#1": "MNILLIGYGAMNQRVARLAEDKGHEIVGVIDRTSKASTPYSQYQHISECKEADVAIDFSNPELLFPLLEEQFNLPLVIATTGEKETLIQKLETLSQRTPVFFSANMSVEIIEAHHRHKVDAPSGTFALPLILGIPYENIEIVIHEQSKIHSMVEYIDGSIVAQAGVHALTKILETAVPLLQDFDIELTEAHHNKKVDAPSGTLVKLYDVIKELRDNVSPVYDRHEKTEKRTHDEIGIHAVRGGTIVGEHDILFAGTDETITISHKAQSKDIFANGAIGAAEKLIHKNPGFYTFNLH",
    "response#2": "IRIAVVGAGGRMGRQLIQAIEQMDGVVLGAALERSGSSLIGSDAGELAGLGKSGITVKESLNAVQNDFDILIDFTRPEGTLAHLAFCHQHRKGMIIGTTGFDDAGKAAIKQAAQDIGIVFAANFAIGVHALTKILETAVPLLQDFDIELTEAHHNKKVDAPSGTLVKLYDVIKELRDNVSPVYDRHEKTEKRTHDEIGIHAVRGGTIVGEHDILFAGTDETITISHKAQSKDIFANGAIGAAEKLIHKNPGFYTFNL",
    "response#3": "MNILLIGYGAMNQRVARLAEDKGHEIVGVIDRTSKASTPYSQYQHISECKEADVAIDFSNPELLFPLLEEQFNLPLVIATTGEKETLIQKLETLSQRTPVFFSANFSVEIIEAHHRHKVDAPSGTIAIEIIEAHHRHKVDAPSGTGVNVMLKLLEKAAKVMGDYTDIEIIEAHHRHKVDAPSGTALAMGEAIADALGRDLKSCAVYAREGHTGERDPKSIGFATVRAGDIVGEHTAMFADIGERVEITHKASSRMTFANGAVRAAIWISSKENGLFDMRDVLGLND"
  },
  {
    "reference": "MQYSEIMVRYGELSTKGKNRQAFIGRLNGNVTRALHEFPNLTIRPKRDRMHIELNGEPSDQVMARLSQVFGIQNFSPSIAVEKDMDKVHAVALQLMNETAPKGISYKVNTRRSDHDFALDTNAMNLDLGDYLTDKRPDLVVKMHQPDMILRVEVRREAIYLSTKTIQGAGGLPVGTAGKAALMLSGGIDSPVAGYYALKRGVDIEMVHFFSPPYTSQQALNKAKQLTAKLTPYVGRIYFIEVPFTEIQEEIKAKVPEGYLMTVQRRLMLRLTEAIAQQRGDLAIFNGESVGQVASQTLESMAAINDVTTMPIIRPVATMDKNEIIAEAEKIDTYDLSIMPFEDCCTIFAPPSPKTKPKTDRARYYESKIDVAGLMDRALAGVKIQEIKSSDQFMNQDQDVIAELL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a specific function in tRNA thio-modification and should be localized to cytoplasm in order to carry out this function efficiently.2. The designed protein must have at least one THUMP domain.3. The ATP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MVKGPSVKKRMVNQLYQNLLALFDRHGLAVTIRRFPDKFEVFCDEVTQSGVEQVLAATPGVDQFLRVVQYTLTTDEPEALMQQVADKLAPHVLDRIEGKTFAVRVKRTGHHRFTSFELERFLGAFLIREGAPAGVQLKQPQETVAMEIHDQCLNVITRRIPGLK",
    "response#2": "MVKGPSVKKRMVNQLYQNLLALFDRHGLAVTIRRFPDKFEVFCDEVTQSGVEQVLAATPGVDQFLRVVQYTLTTDEPEALMQQVADKLAPHVLDRIEGKTFAVRVKRTGHHRFTSFELERFLGAFLIREGAPAGVQLKQPQETVAMEIHDQCLNVITRRIPGLI",
    "response#3": "MVKGPSVKKRMVNQLYQNLLALFDRHGLAVTIRRFPDKFEVFCDEVTQSGVEQVLAATPGVDQFLRVVQYTLTTDEPEALMQQVADKLAPHVLDRIEGKTFAVRVKRTGHHRFTSFELERFLGAFLIREGAPAGVQLKQPQETVAMEIHDQCLNVITRRIPGLV"
  },
  {
    "reference": "MKAIFDQPSFYGIPLDTLKEILKENGHKPFNADQIYSWVYEKLVFDVDGWTNISKDLKSFLTSKFSFELPKVVWNGHSKDGTRKFLVGMRDSKTVETVMIPAKNNRKTLCISSQVGCAIGCTFCHTGTMGLTRHLEAGEIVGQFLAVTKWLKENVSDEERLSNIVYMGQGEPLHNFEHVKNATKVFLEPKGLGLGQRRVTLSTSGLVPQIEKLDDFPPINIAISLHAAHNKIRSELMPINKAYDLTRLLDAIKKIPLKAHRYITYEYLLIEKYNDRAEDIEALIDLLPKKVSKVNLIPFNEYPESSFKRPSVTRINWFKDQLEKGGITCTIRTTKGADILAACGQLKSEMDKKLNLWEEENFTSPT",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must be engineered to have a high affinity for [4Fe-4S] cluster in order to increase its catalytic activity.2. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity.3. The protein should have a high affinity for [4Fe-4S] cluster.4. The protein can be targeted to cytoplasm and perform metal ion binding, rRNA (adenine-C2-)-methyltransferase activity, tRNA (adenine-C2-)-methyltransferase activity.5. The S-methylcysteine intermediate active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "MPPRHLADLDLAGRRAAVAELGEPAFRAQQLSTHYFGRLVRDPAAMTDLPVASRDKIVGDLLPTLLTPQRELTCDDGATRKTVWKLHDNAMVESVLMGYPDRITACVSSQAGCGMACPFCATGQAGLTRNLSIAEIVDQVVYLAGVAASGAVTGSPPRLSRVVFMGMGEPLANYPRVIEAVRRLVAPAPEGLGLSQRHITVSTVGLVPAMRRLIAEELSVTLALSLHAPDDDLRDELVPVNQRWKVAEVLDAAFDYAAQTGRRISIEYALIRDVNDQPWRADLLGRLLHGKLAHVNLIPLNPTPGSKWDASPKPVEREFLRRLRDSGVAATVRDTRGREIDGACGQLAASAR",
    "response#2": "MPPRHLADLDLAGRRAAVAELGEPAFRAQQLSTHYFGRLVRDPAAMTDLPVASRDKIVGDLLPTLLTPQRELTCDDGATRKTVWKLHDNAMVESVLMGYPDRITACVSSQAGCGMACPFCATGQAGLTRNLSIAEIVDQVVYLAGVAASGAVTGSPPRLSRVVFMGMGEPLANYPRVIEAVRRLVAPAPEGLGLSQRHITVSTVGLVPAMRRLIAEELSVTLALSLHAPDDDLRDELVPVNQRWKVAEVLDAAFDYAAQTGRRISIEYALIRDVNDQPWRADLLGRLLHGKLAHVNLIPLNPTPGSKWDASPKPVEREFLRRLRDSGVAATVRDTRGREIDGACGQLAASRTP",
    "response#3": "MPPRHLADLDLAGRRAAVAELGEPAFRAQQLSTHYFGRLVRDPAAMTDLPVASRDKIVGDLLPTLLTPQRELTCDDGATRKTVWKLHDNAMVESVLMGYPDRITACVSSQAGCGMACPFCATGQAGLTRNLSIAEIVDQVVYLAGVAASGAVTGSPPRLSRVVFMGMGEPLANYPRVIEAVRRLVAPAPEGLGLSQRHITVSTVGLVPAMRRLIAEELSVTLALSLHAPDDDLRDELVPVNQRWKVAEVLDAAFDYAAQTGRRISIEYALIRDVNDQPWRADLLGRLLHGKLAHVNLIPLNPTPGSKWDASPKPVEREFLRRLRDSGVAATVRDTRGREIDGACGQLAASL"
  },
  {
    "reference": "MAVASLSICFSARPHLLLRNFSPRPKFVAMAAMSEDPIREWILTEGKATQITKIGSVGGGCINLASHYQTDAGSFFVKTNRSIGPAMFEGEALGLEAMYETRTIRVPNPHKAGELPTGGSYIIMEFIDFGGSRGNQAELGRKLAEMHKAGKTSKGFGFEVDNTIGSTPQINTWSSDWIEFYGEKRLGYQLKLARDQYGDSAIYQKGHTLIQNMAPLFENVVIEPCLLHGDLWSGNIAYDKNNEPVILDPACYYGHNEADFGMSWCAGFGESFYNAYFKVMPKQAGYEKRRDLYLLYHYLNHYNLFGSGYRSSAMSIIDDYLRMLKA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein can be targeted to chloroplast and perform protein-ribulosamine 3-kinase activity, ATP binding, kinase activity.2. The protein's architecture should predominantly feature helix-strand-strand-strand-helix-strand-strand-strand-helix-strand-strand-helix-helix-helix-helix.3. For general function, the protein need meet that Protein-bound erythrulosamine 3-phosphates and ribulosamine 3-phosphates are unstable and decompose under physiological conditions.4. The designed protein should have a binding site that is specific to the target ATP ligand. The designed protein sequence is ",
    "response#1": "SPVLVDYLQTRDLGEVVVVSPDVGGVARARAFAKQMHDAPLAIIDKRRAAHNVAESLTVIGDVAGKTAILIDDMIDTGGTICQGARLLRRNGAARVLCCATHAVFSPPATERLSEPGLFEEIVVTNGE",
    "response#2": "AAPVMYDYLKKKQIEDLVVVSPDVGGLKMAHAYSQVLGSGLAIVAKRRKNALEIESMTVIGEIKGQNVLMVDDLTETAGTLTTAAALLKKEGAKKIIACVSHAILNEIGIERLRKSNIDELITTDPSHGLFF",
    "response#3": "AKVRLKKLEQLLLDGPWRNESALSVETLLDVLVCLYTECSHSALRRDKYVAEFLEWAKPFTQLVKEMQLHREDFEIIKVIGRGAFGEVAVVKMKNTERIYAMKILNKWEMLKRAETACFREERDVLVNGDCQWITALHYAFQDENHLYLVMDYYVGGDLLTLLSKFEDKLPEDMARFYIGEMVLAIDSIHQLHYVHRDIKPDNVLLDVNGHIRLADFGSCLKMNDDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHEERFQFPSHVTDVSEEAKDLIQRLICSRERRLGQNGIEDFKKHAFFEGLNWENIRNLEAPYIPDVSSPSDTSNFDVDDDVLRNTEILPPGSHTGFSGLHLPFIGFTFTTESCFSR"
  },
  {
    "reference": "MSDGLCNGISFNVSEGHGPNYIVGRYKLGFVQQGILFLDPYECLYLYFKGRITFEDSEESALKKLFSITSVERYTAYTLLKNKGYRVHEDNGTIFFRKSTEIPRSVVVVREYDEIDVGKFFTERPDFYFTVDEEGDATIYRIDEIDIKGTENNKIGKCSVTDFGDRHFTKDDLPHWMGTKFHEYRMLTDFEANLIEGKEPKGMAEEVYRDLLSRGCIVKTGFKYGTNFRVYEGEKSEHAEYLVSVVENKLIWYYISRAVRVASSVRKKMIFTTIIDGKIKYIMISRSKDIVI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have tRNA-intron endonuclease activity, lyase activity, nucleic acid binding to facilitate tRNA splicing, via endonucleolytic cleavage and ligation.2. For general function, the protein need meet that Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp .; Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron; The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini3. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "MSGVLRGLAVVVQDVELARALYREGFYGRFLGYDKVKRDEVDKVSAPLILALYEALYLAERGRIKVVAEDGAEIPPEKE",
    "response#2": "MSGVLRGLAVVVQDVELARALYREGFYGRFLGYDKVKRDEVDKVSAPLILALYEALYLAERGRIKVVAEDGAEIPPEKKAT",
    "response#3": "MSGVLRGLAVVVQDVELARALYREGFYGRFLGYDKVKRDEVDKVSAPLILALYEALYLAERGRIKVVAEDGAEIPPEKS"
  },
  {
    "reference": "MPVQAAQWTEFLSCPICYNEFDENVHKPISLGCSHTVCKTCLNKLHRKACPFDQTAINTDIDVLPVNFALLQLVGAQVPDHPIVKLSNIGENKHYEVAKKCVEDLALYLKPLSGGKGIASLNQSALSRPMQRKLVTLVNCQLVEEEGRVRAMRAARSLGERTVTELILQHQNPQQLSANLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRSYEALRREHDAQIVHIAMEAGLRISPEQWSSLLYGDLAHKSHMQSIIDKLQSPESFAKSVQELTIVLQRTGDPANLNRLRPHLELLANIDPNPDATSPSWEQLENAMVAVKTVVRGLVDFIQNYSRKGHETPQPQPNSKYKTSLCRDLRQQGGCPRGTNCTFAHSQEELEKYRLRNKKISAPVRTFPLLNKVGVNSPVSTTAGNVISVIGSTEATGKMVPSSNGIAGLESGSVPQLISRCTDTSIRTLESVKKAGKAGASCQNGSGSPTESLSENKIGSPPKTPVSQAAATSAGGPPIGPEMSSVPPKPSPFITRVPVYPPHSDNVQYFQDPRTQLSYEPPQYPQAGYYPPPPTVPAGVAPCLPRFVRSNNVPESSLPPASVPYADHYSTFPTRDRLNSPYPPPPPQPYGPVPPVPSGMYAPVYDSRRIWRPQMYPRDDIIRSNSLPPMDVMHSSVYQTSLRERYNSLDGYYSVACQPPGEQRTVPLPREPCGHLKT",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Prioritize Polar residues, Pro residues compositional bias within the protein sequence to achieve desired characteristics.2. The protein should be designed to localize specifically to membrane, P-body, cell surface in order to perform its role in T cell proliferation, T cell receptor signaling pathway more effectively.3. The designed protein have C3H1-type, RING-type domains with specific sequence motifs that are crucial for its activity.4. The desired protein should be able to perform S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +   [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-   cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. The designed protein sequence is ",
    "response#1": "VVWEWLNEHGRWRPYTATVCHHIENALREDGRGRVALGQVDAQLTPYVIDLQTMHQYRQDTGTIRPVRPPRVVVWEWLNEHGRWRPYTATVCHHIENALREDGRGRVALGQVDAQLTPYVIDLQTMHQYRQDTGTIRPVRRNFFEPSSSKTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQINNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNSLMWIKENDPRILELTFCLDEDVFGQKSQHELKTGGANIEVTNENKDCTICMERLVTASGYDGVLSHRGIRAELVGKLGKCNHMYHVLCPVAMYNNGNKDGSLQCPTCQVTKTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQINNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNSLMWIKENDPRILELTFCLDEDVFGQKSQHELKTGGANIEVTNENKDKTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQINNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNSLMWIKENDPRILELTFCLDEDVFGQKSQHELKTGGANIEVTNENKDCIRKVFITYSMDTAMEVVKFVNFLLVNGFQTAIDIFEDRIRGIDIIKWMERYLRDKTVMIIVAISPKYKQDVEGAESQLDEDEHGLHTKYIHRMMQIEFIKQGSMNFRFIPVLFPNAKKEHVPTWLQNTHVYSWPKNKKNILLR",
    "response#2": "HLRIVVLSGQSLAKKDIFGASDPYVRIDLNTINGDINIDSVLTKTKKKTLNPTWTEEFIFRVKPSEHKLVFQVFDENRLTRDDFLGMVELTLVNLPTEQEGRTGEIHLPLRPSDVTKTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQINNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNSLMWIKENDPRILELTFCLDEDVFGQKSQHELKTGGANIEVTNENKDYTLGKRKVFITYSMDTAMEVVKFVNFLLVNGFQTAIDIFEDRIRGIDIIKWMERYLRDKTVMIIVAISPKYKQDVEGAESQLDEDEHGLHTKYIHRMMQIEFIKQGSMNFRFIPVLFPNAKKEHVPTWLQNTHVYSWPKNKKNILLRHERKPRTVGQ",
    "response#3": "VIPDDFRCPISLEMMRDPVIVSSGQTYERTCIEKWIEGGHSTCPKTQQALTSTTLTPNYVLRSLIAQWCEANDICN"
  },
  {
    "reference": "MTDHSEFDSVSGKTTTGHEWDGIKELNTPLPRWWVICFYLTIVWAIGYWIVYPAWPLISSNTTGLFGYSSRADVAVELANLEKIRGDKMAALGAASLADVEKDPALLALARAKGKTVFGDNCAPCHGSGGAGAKGFPNLNDDDWLWGGTLDQIMQTIQFGARSGHAKTHEGQMLAFGKDGVLKGDEIVTVANYVRSLSGLPTRKGYDAAKGEKIFVENCVACHGDGGKGNQEMGAPNLTDKIWLYGSDEAALIETISQGRAGVMPAWEGRLDPSTIKAMAVYVHSLGGGK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that can perform electron transfer activity in periplasmic space to support oxidative phosphorylation, proton transmembrane transport.2. The heme c binding site should be stable and able to withstand changes in environmental conditions.3. The designed protein should contain Periplasmic topological domains.4. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.5. The designed protein have Cytochrome c domains with specific sequence motifs that are crucial for its activity.6. The protein should be optimized for activity in Energy metabolism, oxidative phosphorylation pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "TPAMTIKVIGLQWYWKYEYSDFVDSIGETIEFESYVIPDDMLEPGALRLLDTDTSIVVPVDTHIRFVVTANDVIHSFTIPSLGMKIDATPGRLNQVSALIQRTGVYYGQCSELCGVNHGMMPIKLECVSIEDFIEWLGD",
    "response#2": "NAEGIHELYDHIMFYLCLILGLVSYILYVIIKDYKDNRFAYKYVRHGQVIEIIWTIFPAVILLLIAFPSFILLYLCDEVLTPAMTIKVIGLQWYWKYEYSDFVDSIGETIEFESYVIPDDMLEPGALRLLDTDTSIVVPVDTHIRFVVTANDVIHSFTIPSLGMKIDATPGRLNQVSALIQRTGVYYGQCSELCGVNHGMMPIKLECVSIEDFIEWF",
    "response#3": "GISSNYDRLPFAPYYIFKDLITIFLFFFVLSLFVFFMPNALGDSENYVMANPMQTPPAIVPEWYLLPFYAILRSIPNKLLGVIAMFSAILIILVMPITDLSKYRGLQFRPLSKVAFYVFVANFLILMQLGAKHVESPFIEFGQISTVLYFSHFLVIMPLVSFIENTLVELI"
  },
  {
    "reference": "MILVIAEKPNVARKIAGALSERRPIRKTIFGVPYYEVFRDGKKLIVASAVGHLYGLAPKQDFFGYPIFDIEWVPVYIAEKNKDYAKDYIKALSVLAKRVKEFIVACDYDTEGEVIGYTALKYACGVDPSRAKRMKFSALTKRDLLNAWRNLEPTINFGMANAGIARHVLDWYWGVNLSRALTHAIKRASGKWVVLSTGRVQGPTLKFLVEREREIQSFVPKPYWVIKLVIEKNGQKLVANYEKDKIWSEEEAKKIVTEVKKSKARVSNIEVKRQKRNPPVPFDLGTLQREAYSAFGFSPKKTLDIAQSLYEKGFTSYPRTESQKLPKNLNFRLIIQNISRMPQYRPYAHLLLGMPELKPVEGKKEDPAHPAIYPTGEIPGPGDLSKDEEKLYDMIVRRFLALFMEPAVRESVKVTILAGPHKFMLSGARTVKQGWLSVYGKYIKFDEVTLPEFFIGERVRVLQVRREKKKTKPPARYSPAAVIKKMEDLGIGTKATRAQILETLYQRGYIEGKKSIKVTPLGMKVIETLEKYVPEIISVELTREFERKMDLIMQGKLTKEEVIEEAKVKLTKILEEFKKRELEIGLELARIVVGDVKDEIKEEKPIVVGKCPKCGGDLIVKYNKKTGKRFVGCSNWPKCDVTYPILQRGEIIPTNKTCCNGAPVVIIRDKGRDIEICLDIKCKKW",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have a specific function within the chromosome.2. The protein must exhibit the following characteristics: Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.. The designed protein sequence is ",
    "response#1": "HEVIICEKPKSSEKIAGALFPDAMKKKHGKVSYWEHVEGDKRVTIVSAVGHLYSLRPRQSNEEHFFDLEWAPIHEIDKKKGYVKDYLNVIRKFAAGADRYIHACDYDIEGTLIGFNALKYGCGEEC",
    "response#2": "MDIRTVLKDNFLQYSSYVIKDRAIASVVDGFKPVQRRIIHSLFEMHDGNFHKVANVVGNTMKYHPHGDTSIYEALVNIANKDLFIEKQGNFGNLFTGDPASASRYIECRLTPLAFDVLYSKEITIYESSYDGRNNEPLLYPAKIPVILIQGSEGIAVGMAAKILPHNFNEILNAVKSELLGESYDIYPDFPTGGIVDVNEYADGNGKVLVRAKIETIDEKTIVIRELPFGETTESLISSIEKAIRKNYIKVSSINDFTAENVAIELSLPRGVYASEVIEKLYHYTNCQISISVNLLLLSERYPVVYTIKDLIKFHAAHLQKILKMELELQKSKILEKIFYKTLEQIFIEKKIYKLLETISKEENILSIILSEVLRHKESFSREVLKEDVENLLKIPIRKISLFDIDKNSKDIKILNKELKSINSNISSIRGYA",
    "response#3": "MDIRTVLKDNFLQYSSYVIKDRAIASVVDGFKPVQRRIIHSLFEMHDGNFHKVANVVGNTMKYHPHGDTSIYEALVNIANKDLFIEKQGNFGNLFTGDPASASRYIECRLTPLAFDVLYSKEITIYESSYDGRNNEPLLYPAKIPVILIQGSEGIAVGMAAKILPHNFNEILNAVKSELLGESYDIYPDFPTGGIVDVNEYADGNGKVLVRAKIETIDEKTIVIRELPFGETTESLISSIEKAIRKNYIKVSSINDFTAENVAIELSLPRGVYASEVIEKLYHYTNCQISISVNLLLLSERYPVVYTIKDLIKFHAAHLQKILKMELELQKSKILEKIFYKTLEQIFIEKKIYKLLETISKEENILSIILSEVLRHKESFSREVLKEDVENLLKIPIRKISLFDIDKNSKDIKILNKELKSINSNISSIRGYR"
  },
  {
    "reference": "MAQKENAYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTGTWGKGERTDERLWEGLGMRRSSLLSFNLPVVSLAAPGQKVVENSSGTPNILMRHFTIDDFEIGRPLGKGKFGNVYLAREKKSHFIVALKVLFKSQIEKEGVEHQLRREIEIQAHLHHPNILRLYNYFYDRRRIYLILEYAPRGELYKELQKSRTFDEQRTATIMEELADALMYCHGKKVIHRDIKPENLLLGLKGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRTHNEKVDLWCIGVLCYELLVGNPPFESASHNETYRRIVKVDLKFPASVPTGAQDLISKLLRHNPSERLPLAQVSAHPWVRANSRRVLPPSALQSVA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must have at least one Protein kinase domain.2. The ATP binding site should be stable and able to withstand changes in environmental conditions.3. The protein with the reaction ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-   threonyl-[protein], the Proton acceptor must be optimized to ensure efficient catalysis.4. A protein that contributes to positive regulation of lateral attachment of mitotic spindle microtubules to kinetochore, negative regulation of transcription by RNA polymerase II, positive regulation of telomere capping, positive regulation of telomerase activity, positive regulation of telomere maintenance via telomerase by functioning in chromosome passenger complex, mitotic spindle midzone, mitotic spindle pole, nucleoplasm with ATP binding. The designed protein sequence is ",
    "response#1": "AWTLENFDIGRALGKGKFGSVYLAREQQTKFILALKVLFKKQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDAARVYLILEFAPKGELYGELQRCGTFDDQRSATYIMELADALRYCHSKAVIHRDIKPENLLLGANGELKIADFGWSVHTPSSRRSTLCGTLDYLPPEMIEGKTHDEKVDLWSLGVLCYEFLVGRPPFETKSHEETYRKISRVEFTYPAHVSNGSRDLINRLLKHNPMHRLPIQGVMEHPWVEMLGKGKFGNVYLARERQSKFILALVSLWGIGKPLGRGKFGHVYLAREKRSNHVVALKVLFKSQLQQSQVEHQLRREVEIQSHLRHPNILRLYGYFYDQKRVYLILEYAARGELYKDLQKCKYFSERRAATYVASLARALIYCHGKHVIHRDIKPENLLIGAQGELKIADFGWSVHTFNRRRTMCGTLDYLPPEMVESVEHDASVDIWSLGILCYEFLYGVPPFEAMEHSDTYRRIVQVDLKFPPKPIISASAKDLISQMLVKESSQRLPLHKLLEHPWFTATEG",
    "response#2": "IGRALGKGKFGRVYMVRTKAPPQFIIALKCLYKRELVECKVEKQLRREIEIQSNLRHPNILRLYGYFHDEKRIFLMLEYAGKGELYKQLHRHGKFSEKRSAKYIAQMADALHYLHRKHVIHRDIKPENLLLGVEGELKIGDFGWSVHAPGNRRKTLCGTLDYLPPEMVEGKDHNEKVDLWALGVLTYEFLVGVPPFEDLSGHNATYKKISKVDFSIPPTVSSEASDLIRKLLRHEPQDRLPLPQVANHPWIN",
    "response#3": "FDIGKPLGRGKFGHVYLAREKRSNHVVALKVLFKSQLQQSQVEHQLRREVEIQSHLRHPNILRLYGYFYDQKRVYLILEYAARGELYKDLQKCKYFSERRAATYVASLARALIYCHGKHVIHRDIKPENLLIGAQGELKIADFGWSVHTFNRRRTMCGTLDYLPPEMVESVEHDASVDIWSLGILCYEFLYGVPPFEAMEHSDTYRRIVQVDLKFPPKPIISASAKDLISQMLVKESSQRLPLHKLLEHPWL"
  },
  {
    "reference": "MKSKNVVIGVTGGIAAYKALDVVSHLKKAGFEVDVIMTNSACKFVAPLSFQSLSGNKVITDMFEEPKSFEIQHISLAKKADIFVVVPATANIIGKVANGIADDMLSTTIMAAYGRCPIAFAPAMNTNMYKNPIVQNNIKKLREYGYRFIDPESGRLACGDVGEGKLADVDLISKTIESMLYEKKDLAGKKVMVTAGPTIAPIDPVRYITNHSTGKMGYAIAEEARDRGAEVTLISGKTNIEPPFGVKFVKVATNAEMLKEVMDNFPKSDIVIKAAAVSDYKVKNYSEIKIKKNDDSLNIEFVKDNDILRKLGQIKKNQILVGFAAESNDLIENARKKLSKKNLDYIVANNILAKDTGFASSENKVVILDRDGGKLELEKMSKRKVAENLFDLLSKKRS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein with phosphopantothenate--cysteine ligase activity, metal ion binding, phosphopantothenoylcysteine decarboxylase activity, FMN binding is needed to enhance pantothenate catabolic process in a specific context.2. A protein that is capable of catalyzing reactions within the Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway with high efficiency and specificity.3. The Proton donor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.4. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal domain.5. The designed protein should have a binding site that is specific to the target CTP ligand.6. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A. The designed protein sequence is ",
    "response#1": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALDWARATLDDGPLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHVLARDGTVLEGLNVVVTAGGTREPIDAVRVIANRSSGKQGHAISEECVRRGANVTVVTTTELPTSPTTDRIEVETAAEMQIALDNVAPTADIVVMAAAVADFRPKDSVDGKIKKAGGVPEVILEPTPDLLEAIGRNRHPHQTIVGFAAETCDLVTNARSKLERKCLDLVVANDVSAEGVGFAHGTNAVTLLGADGSETPVPHGSKALLAAAVWDAVAAR",
    "response#2": "MKGKKILVTAGPTRELIDPVRFISNPSSGKMGFAIARAAEQRGGDVILITGPTGLPDPNGVTVVRVGTAREMALAVFGHMEDSEIIIKTAAVSDYSPKNQAGQKIKKQKDELVLLLEKTQDILKEVGRRKKDRIIVGFAAETENLEQYAGKKLVEKNLDIVVGNLVGRSSSGFGTDTNEVTMADGTLLALKGRHIIVTSGPTHEPIDPVRYIANRSSGAQGTALARALSALGADVTFISGPADVPPPAGVNVVNVQTAQQMKDAVEAALPADAAIFAAAVADWRVANASGSKMKKDGSGNAPTLEFAENPDILAGVSQLKQNRPKLVVGFAAETDDVEVNATAKRARKGCDWIVANDVSPETGIMGGSENAVTVLVSAGEVKELPLMSKQNIAHQILDYVAQQFA",
    "response#3": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALRLLAGPAPAAELAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHVLAADGTEELRGLKVVVSAGPTYEDIDPVRFIGNRSSGRMGFAVAEAAAQAGAEVTLVAGPVSIATPPGIARRIDVRSAAQMRDAVVTASAGADIYIAAAAVGDYRPAEVADHKLKKSDGAPLELRLTENPDILVTLAAQTVRPFLVGFAAETHDVERYAQDKLKRKGLDMIAANQVGGGLGFEAADNALTVLWRDGAVEELPRLGKREVADRILDRVVRLLG"
  },
  {
    "reference": "MSESFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSRDEDPYCVACFGELFAPKCSSCKRPIVGLGGGKYVSFEDRHWHHNCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Recruited by SOX15 to FOXK1 promoters where it acts as a transcriptional coactivator of FOXK1..2. The designed protein should have transcription coregulator activity within focal adhesion, Z disc to regulate muscle organ development. The designed protein sequence is ",
    "response#1": "IPQESPEHGMMASPQMYNRQMYVSECAKTHPMAQTEQHFPSYQSQKTVRQNYLQSQQDGHLESDIDKTEFDQYLMYEPKADMEIIYTIDQDSGAYSTNLLPSLITEANNVCYYDYCGN",
    "response#2": "PKDLVKPPYSYIALITMAIQNAPEKKITLNGIYQFIMDRFPFYRENKQGWQNSIRHNLSLNECFVKVPRDDKKPGKGSYWTLDPDSYNMFENC",
    "response#3": "PKDLVKPPYSYIALITMAIQNAPEKKITLNGIYQFIMDRFPFYRENKQGWQNSIRHNLSLNECFVKVPRDDKKPGKGSYWTLDPDSYNMFENC"
  },
  {
    "reference": "MNPNEVAVFSGNANRPLAEEICNQLGIPKGAISVKRFSDGESSVKIEENVRGRDVFVVQSISFPANDSLMELLLIIDALRRASARRITAVLPYYGYGRQDRKVEPRVPISARVVADLIETVGPNRVLTMDLHADQIQGFFRIPVDHLYFSPVLAEYINSLKMDDLVIVSPDSGGAERARNFGKKVNGSLAIIDKRRPKANESVVMNVIGDIKDKNCLLLDDMIDTGGTIAKAAMALYENGAKSVLCCATHGVLSGEAPEKLNQANFKQIVLSNSIMIPETKKINNLKTLSIAPLFAKAIERIHKEESISSLFL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be able to bind ATP ligand in a variety of conditions2. The protein design should take into account the physiological conditions of the 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1 pathway.3. The protein should be optimized for stability and expression in cytoplasm where it will participate in nucleotide biosynthetic process, 5-phosphoribose 1-diphosphate biosynthetic process. The designed protein sequence is ",
    "response#1": "IPLFAEYYNEKGLTGADVVVVSPKNSGVKRARSLAEYLDAPIAIIDYAQDDTGREEGYIIGEVEGKTAILVDDILNTGKTFAEAAKILERGGVNDIYAVASHGLFAGGAGEVLNASPIKEILVTDPE",
    "response#2": "LLSDYWKKKSLKDLVVVATDIGITKRARDLAAKLHAPLAIIEKRRIGNIDATETLNIIGEVEGMRALTVDDEIDTAGSLVGAVNTLLEHGATEVYACCTHPIFSGPAIERIASSPVKEVVVTDSR",
    "response#3": "MIVIVGPSGAGKDTLMDYAAAQLSGRPGFHFTRRVITRSCDAGGENHDAVSMHEFNQLEDAGAFAVSWQAHGLKYGIPAAVYRHLEAGDVVIANGSRSALPHFGTAFSRLKVVNIVARPDVLARRLEQRGRESRDDILRRLERSSLAVAGDFDVTTVDNSGAIEDAGKTIMQVLK"
  },
  {
    "reference": "MPSVLASCPPVLSCAAAQALESDLFGGDEAREWAAMKHAGAAVAAGIVEDLREIGGLRAGVRLLVLVGKGHNGGDALIAAGELLQRFPDARADIVFVFGPRGLRPLAVRAWRELAQSARERVGTLSPSEVGRQGAAYAVALDGVFGFQFRPPIAPEVAALLQAVNALPIALRAAVDLPSADLFQADFTYATGSVKQPLLGSEAAGRVRYLDLGFFRGGAAADASDRVLTGALLDPLRRFRPMRSDKRTYGHVFLLGGSRSFPGAILMSAHAAIRSGAGLVTAFVPESVAAAFAARLPEVIWVGWPETPGGGLALEGEHLWRERLERADAAVIGPGLGREPETLALAQSLVASATVPVAIDADALQPEIVRARRGLAPRVVTPHAGELARIAGAADLRTFAEETGLTVVAKGPVTRICAGGPVYHSFFGGPVLARGGSGDLLAGMIGTQLAQPWDDPLLAAARAVVWHGLAADALARAEGAVAVETTRLLDYLGAVLR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must be able to perform ATP binding, NADHX epimerase activity, NADPHX epimerase activity, metal ion binding, ADP-dependent NAD(P)H-hydrate dehydratase activity under specific conditions.2. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.3. The designed protein should be able to compete with endogenous proteins or inhibitors for (6S)-NADPHX binding.4. The designed protein must have at least one YjeF N-terminal domain.5. For general function, the protein need meet that Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP; Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.6. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "MRKADASVIRSGTPGVTLMQNAGAAVADAAREMVSPGARIVVLAGPGQNGGDGIVAATRLREQGYRVSLGLLGKREAFSGDAALVAREWPSDVVAVDGANLAPAELVIDALFGGGLSREIEGAARAAIDRINMSGGPVLAVDLPSGIDGETGAVRGIAVRAARTVTFAARKPGHYLLPGRTFCGDVTVADIGVGSSDLPSHATRLVADGFEAHKRYIPIDPFTHKGKQGHAIIIGGSYGKIGAVCLAAKSALKSGCGLITAYLPKCGYVVIQTAFPEAMAITDGDEHITHINFDFEPKAIGIGPGMGQHPETQKAFHDFLIRYKHPLVIDADGLNILSYNKEWLELLPENTILTPHPKELERLMGAWNDGFDKIEKMKVFCKKYHVILVAKDARTMVVYGNEVHINASGNAALATGGSGDTLTGIITGLLAQGYTAADAAVFGVYLHGLTADIALPKTGMQAFTASMIPDYIGKAYHEGAADVAWMAQPRKLDAHKGTFGHVLILAGSVGKTGAAALAARAALRAGAGLVTVATAESALPVIASLGMEFMTEPLPETDSGTISLRALDGGRLDKLVEGKSVLAIGPGIGSAPETAEVVRAAVNKYDLPVVLDADGLNAFAGCMSGFRASGRARILTPHPGEMARLAGLTTAEIQAHRVEVARDFASRYNVYLVLKGFRTLTAAPDGQVCVNPTGNPGMATGGTGDVLTGLTAGLLSQFAGRPAIDVVAGAVYLHGLAGDLAAAELGQHSMIAGDLVEALPRAFRA",
    "response#2": "MRKADASVIRSGTPGVTLMQNAGAAVADAAREMVSPGARIVVLAGPGQNGGDGIVAATRLREQGYRVSLGLLGKREAFSGDAALVAREWPSDVVAVDGANLAPAELVIDALFGGGLSREIEGAARAAIDRINMSGGPVLAVDLPSGIDGETGAVRGIAVRAARTVTFAARKPGHYLLPGRTFCGDVTVADIGVGSSDLGPVAGSGDVLTGIAAEEAGDLLPVRDPMAHKKSVGQVLVIAGSRGMSGAAVLTGHAAYKMGAGLVAFAPPQGIAPVLNAALLEAIVWPQPETEAGTLFWDARSSLLELAGQYDVVALGPGLSTNPQTVEFVEKLVADIEKPLVIDADALNALAGDAGILKSREALTVITPHPGELARLYGVSSKDVLADWLGFARRAQERFGAVVVLKGASTVICSAEETTINVTGNPGLATAGTGDVLTGFIAALIAQGVDAYNAACLGVYLHGFSADIAAEDIGELSLMASDVIEYLPEAIPRPGPSSHKYERGHVLVASGGATRTGAARLAARAALRIGAGVVTVASPPEALGVNAAQLTAIMLRGCDGPEGLHAILADERLNALVVGPALGVTAVTRAMVAVAAQARRRLVLDADALTSFEGLASELHQGFRSAPTVITPHDGEFTRLFKGHPDILDPSSKVERARRAAAYLSAVAVLKGADTVIAAPDGRAAINANGTPYLGTAGSGDVLCGIIAGLMAQHLPAFEAAAAAVWIHAEAGAAFGAGLISEDLPELIPEVVLVAGGERGFSGAVQMAASAALRVGAGLVSLATRSEHAALLNLVRPELMVHGIDKRVDLAPLLKKSSVAAVGPGLGQTVWAQNLLAELFDTAMPLVVDADALNLLAKEPFKRDNWVLTPHPGEAARLLGITIKEIESDRFSSVKALQKKYGGVVVLKGAGTLIFDGSEMVINTTGNPGMASGGMGDVLSGVIAALLAQGLSLSQSARLGVWLHGKAGDIAAKEGERGLLASDLL",
    "response#3": "MRKIDSYCINELGISELILMENAALKIINNIDLKNNMSFTVVCGSGNNGGDGLAVARHLISLGKAVQVFFVGNIDKMSDSCKVNYLILKNMKINIINIISLEYIEALNLAVARSDITIDALLGTGISRDVQGIHTLVISVINENTNFTLAIDVPSGLNSNNGNKMGNCIISNKTVTFEFYKSGFLNYDSEKYTGEIIVEKIGIPQFVIDGFEAHKRYIPIDPFTHKGKQGHAIIIGGSYGKIGAVCLAAKSALKSGCGLITAYLPKCGYVVIQTAFPEAMAITDGDEHITHINFDFEPKAIGIGPGMGQHPETQKAFHDFLIRYKHPLVIDADGLNILSYNKEWLELLPENTILTPHPKELERLMGAWNDGFDKIEKMKVFCKKYHVILVAKDARTMVVYGNEVHINASGNAALATGGSGDTLTGIITGLLAQGYTAADAAVFGVYLHGLTADIALPKTGMQAFTASMIPDYIGKAYHEVLIFAGSKKYTGAAYICTEGAVRSGAGLITLCCDEDILQILKNKLAEAMTISSKDNDGIDKLLLNSNCIAIGPGMGNNENTFKIVNYVLKASNCPVVIDADGINVLSNNLGILNNVKVPVVLTPHLGEMARLTGFSIEYIEQNKMKVVKDFAITYKVIVLLKGYNTIITDGNIAIVNPTGNSSMASGGMGDCLTGIITSFIAQGQKPIIAAMTGAFLHGYCGEMLSREMFNVSATDILNKIVLILAGSSKYPGAPILTALGAQRIGAGLVTLITPGDSSKILSFEPSIIYSSLNKNNFEKNDLKKLNDEIERANVIILGPGMTEDSIEFVVELIKKQGRTKNIVLDAESIQAIKEFESLSENILITPHVGEMKKIFKNLKNNIFELERFSKQKKCIIMLKSSITTITDGEKTFFNLLGSSSLSKGGSGDLLSGIVGGLIAQGLDTINACITGSYIMYKTAKELSNEYTEYFITPKLIS"
  },
  {
    "reference": "MLPVPDTAIIVVAAGSGTRLDAGAPKAFVGIDSRSILRHALDGVFAAAPAQVIVVAPAGFEGDAETELREAAGERIDLGRVVTGGDTRQLSVSAGLAALWGGVTTVLVHDAARALTPPAQIDAVARAVRADTGVIPALPVVDTLKRVDDGLIVAAVDRSELAAAQTPQGFPRGPLQAAYAAALTAGVEYTDDAALFAANGGSVRHIEGSERAFKITTPGDLERARHLLADAGAPAPASHPASEGLPASVAPRVGIGTDVHAFGGDGALWLAGLEWPGEPPLSGHSDGDAVAHAIVDALLGAAGLGDIGEHFGTSHPEYAGAHAAVFLARTRELLAEAGFAIGNVSAQFQGNRPRFSARRAEAEQVLSAALGGAPVSVTATTTDGLGFPGRGEGIAVTAIAMLLPTGLTQGHVTPPR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be optimized for activity in Isoprenoid biosynthesis, isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6, isopentenyl diphosphate biosynthesis via DXP pathway pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The designed protein have 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domains with specific sequence motifs that are crucial for its activity.3. A protein with 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity, metal ion binding is needed to enhance isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway in a specific context.4. The a divalent metal cation, 4-CDP-2-C-methyl-D-erythritol 2-phosphate binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MRVGTGFDVHRLADGEDLWLCGVKIDHHQGLAGHSDADVGLHAVVDAILGACARGDIGDHFPPSDPQWKGAASSAFVQHAVKLAGEAGYAIGNVDLTLICEAPKIGPHRGAMAVRLAELLGIPVSAVSVKATTTEKLGFTGRGEGIAAQAVTTLVEAQAVED",
    "response#2": "MRVGTGFDVHRLADGEDLWLCGVKIDHHQGLAGHSDADVGLHAVVDAILGACARGDIGDHFPPSDPQWKGAASSAFVQHAVKLAGEAGYAIGNVDLTLICEAPKIGPHRGAMAVRLAELLGIPVSAVSVKATTTEKLGFTGRGEGIAAQAVTTLVEMPAM",
    "response#3": "MRVGTGFDVHRLADGEDLWLCGVKIDHHQGLAGHSDADVGLHAVVDAILGACARGDIGDHFPPSDPQWKGAASSAFVQHAVKLAGEAGYAIGNVDLTLICEAPKIGPHRGAMAVRLAELLGIPVSAVSVKATTTEKLGFTGRGEGIAAQAVTTLVEVACEQ"
  },
  {
    "reference": "MTQSTDDTVSFGFTEVTPEEKTRKVGEVFSSVARKYDIMNDAMSFGMHRGWKDRFVKRVKPQPGENILDMAGGTGDIAFRMADRGANVTVADINQEMLDVGAERAMERDEPEGAGSLVFSKQNAEEVTFAANTFDAYTIVFGIRNVTFKDKALAEAYRVLRPGGRFFCMEFSVTDWAGFREIYDLYSMHVMPRMGQVIAGDTESYRYLAESIRKFPRAPEFEAMIRTAGFVNTKAEIILGGAVAIHSGWKI",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be optimized for activity in menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2, menaquinone biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The designed protein must be able to perform S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity, 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity, S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity, demethylmenaquinone methyltransferase activity under specific conditions. The designed protein sequence is ",
    "response#1": "MTDLPRYAIIVVAAGSGTRAGGAVPKQWQPLAGRRVADWTLDAFAAMAPDARVVLVLGPGQEEAARAALGAGWRLAAGGATRAASVRAGLEALAARAPDRRVTKPGGRLVVCEFRPCVPAGLIDRVLAALDEHPGAIPALPVSDTLKRGEGAMVAGTVPRDGLFRAQTPQGFDFARILAAHRAHTGAATDDAAVAEAAGLAVALTPGDERNLKITHPDDLARAAA",
    "response#2": "MTDPIADMLTRIRNGQAANKTSVRMPSSKLKVAIADILKREGYIADYAVSDGKPTLEINMKYFSGKPVISGLKRISKPGLRVYAKSDEVPSVKNGLYRVLKRGGRLLVLEFTRAGLYAQTKVAGEAICYVA",
    "response#3": "MSEIKFNTIEEALEDIKAGKMIILVDDEDRENEGDFIMAAEKVTPEAINFMAKHGRGLICLALTEERCDELELPLMVGKNTAAFGTAFTVSIEAREGVTTGISAYDRAKTILTAIDENSTPGDLARPGHVFPLRAKEGGVLRRAGHTEAAVDLARLAGLDPAGVLCEVMNEDGTMARLPQLMKIADKFGLKIITIKDLIAYRMRKESLVREVRVLKPGGKLLVLEFPIEIAPNECNEKYLATKRDKMGHLLT"
  },
  {
    "reference": "MIKYYNRKTKNYEIEKIAGEKYLNWTYNSPVGMTILEKFIKKKLFSKIYGLYCNSKISSYKIKKFATDFNINMSHSEKSFNDFKSFNDFFSRKLIKSSRPIADNKNFLISPCDGKIIVYNNINMNNIIQVKGITYKLNDLIQNDSISKTYEKGTCIIARLCPTDYHRFHFLDNGICESTIKIKGNYYSVNPIALNKINNLFCQNKREWSIFNSDNFGKILYVEIGATCVGSIIQTYMPNKKVLKGMEKGYFKFGGSTIVLFLEHNKVIIDEDILTESKLGIETKVLMGERIGTKLN",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must be engineered to have a cooperative binding mechanism with pyruvate to ensure proper regulation.2. A protein that contributes to phosphatidylethanolamine biosynthetic process by functioning in plasma membrane with phosphatidylserine decarboxylase activity.3. The protein with the reaction a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-   diacyl-sn-glycero-3-phosphoethanolamine + CO2, the Charge relay system; for autoendoproteolytic cleavage activity, Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity must be optimized to ensure efficient catalysis.4. The protein must exhibit the following characteristics: Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer)..5. The designed protein should interact with enzymes and substrates within the phosphatidylethanolamine from CDP-diacylglycerol: step 2/2, phosphatidylethanolamine biosynthesis, Phospholipid metabolism pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "VAGMSPGDTRGDHKGLGLLLRVNVLKGRNLAAKDKRGTSDPYLVIALGDRKEATSVVSRTLNPEWNQVFEFPVTDTESALLEATCWDKDRFKKDYMGEFDVLLEDLFANGSTAPEPVWYNFRNNKVLLRANVIQGRNLAAKDRSGTSDPYLVLNVGDAREATPPINKTLNPEWNTTLEFPIVGTQSMLLEACCWDKDRFGKDYMGEFDVIVEDVFQSGQAKPNKAGPPTNVLFLEIHRITDLPPERNMTRTSFDMDPFVVTSLGKKTYRTKVVRHELNPVYDEKLVFQVQKHELNYSLSFAVVDRDKFSGNDFVGTGLFPVQKLLRVNVLKGRNLAAKDKRGTSDPYLVIALGDRKEATSVVSRTLNPEWNQVFEFPVTDTESALLEATCWDKDRFKKDYMGEFDVLLEDLFANGSTAPLLRVNVLKGRNLAAKDKRGTSDPYLVIALGDRKEATSVVSRTLNPEWNQVFEFPVTDTESALLEATCWDKDRFKKDYMGEFDVLLEDLFANGSTAPA",
    "response#2": "MSRKSKLKTYEFSGMSDVSGVLFLEIHRITDLPPERNMTRTSFDMDPFVVTSLGKKTYRTKVVRHELNPVYDEKLVFQVQKHELNYSLSFAVVDRDKFSGNDFVGTGLFPVQKVRSLAPVADEEE",
    "response#3": "MKIVLFLEISKVTDLPPEKNGKCTFTRTSFDMDPFVVTSLGKKTYRTKTIRHNLNPVFDEKLVFQVLRHETNYSVSFTVIDKDKFSGNDYVGTVNFPVEKAVSVAPEADGDGSADAPSTNAAGKNLRVNVLKGRNLAAKDKRGTSDPYLVIALGDRKEATSVVSRTLNPEWNQVFEFPVTDTESALLEATCWDKDRFKKDYMGEFDVLLEDLFAGVLRANVIQGRNLAAKDRSGTSDPYLVLNVGDAREATPPINKTLNPEWNTTLEFPIVGTQSMLLEACCWDKDRFGKDYMGEFDVIVEDVFQSGQAVQLF"
  },
  {
    "reference": "MLNRLESLTQRVGGSNELIDQWLHARKELLVSYCTVIGIKPQKGKHTPLNEKALENFCHNLVDYLSSGHFHIYDRIIKQVEGASSPKMALTTKVYPALKNNTQTIMAFHDRYTNIEIDDDSCTEFQQALSDIGEALDARFRLEDQLIQWAAESWQAAKPVIQAGQVK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Binds RpoD and negatively regulates RpoD-mediated transcription activation by preventing the interaction between the primary sigma factor RpoD with the catalytic core of the RNA polymerase and with promoter DNA; May be involved in replacement of the RNA polymerase sigma subunit from RpoD to RpoS during the transition from exponential growth to the stationary phase.2. The designed protein localizes to the cytoplasm. The designed protein sequence is ",
    "response#1": "METRTI",
    "response#2": "MEIVRL",
    "response#3": "MSFLGL"
  },
  {
    "reference": "MLGIGTLAKKVFGTPNDRKIKATRPLIAKINALEPEFEALSDQGIKDKTEDLRKRALAGESLDDLLPEAFANVREAARRALGLRAFDTQLMGGTFLHQGNISEMKTGEGKTLVATFPAYLNALTGKGVHVVTVNEYLAKRDSEWMSKVFGALGMTTGVIYSNQPEAEKMAAYQCDVTYATNNELGFDYLRDNMKPSLDQVFQKQHNFAIVDEVDSILIDEARTPLIISGPAEDRSELYETIDKLIPTLDEGHYEIDEKTRGVTFTDEGNEFLEESLLKAGLLEEGASLYDPESTTIVHHVNQALRAHKLFQRDKDYIVRDGNVVLIDEFTGRMMPGRRLSEGLHQAIEAKEGTDIQPENTTLASVTFQNYFRLYDKLSGMTGTAMTEAEEFAEIYGLGVVEVPTNRPIARIDEDDKVYRTANEKYAAMIAETKLAHEKGQPVLLGTTSIEKSELLSQLLQQEGIEHNVLNARHHEQEAKIVAEAGRLGAVTIATNMAGRGTDIQLGGNIDLKVMEALEANPDADPAVLRAEEEAKHAEEKQKVLEAGGLYVMASERHESRRIDNQLRGRSGRQGDPGRTSFYLSLEDDLMRIFGSERLDKLLSGLGMKEGEAIIHPWVNKSLERAQAKVEGRNFDMRKNVLKFDDVMNDQRKVVFAQRREIMASDDTHEIVTDMRHEVIDDLIDIYMPPKTYADQWDTQGLQDDVREKLNIDAPVVEWAAEEGVDDEQIRERLVEASDKLMAEKVEAFGQEGMSNIEKQVLLQTIDAK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be able to bind ATP ligand in a variety of conditions2. The protein must exhibit the following characteristics: Interacts with the SecYEG preprotein conducting channel; Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane..3. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "RLLGASTDKNLNYSSAEVTAAAAYHKEAADLGDEQLRKACGLLNLNDLADSRDVPQFLAIVREASERSTGLRPFDVQLLGALRMLAGDVIEMATGEGKTLAGAIAAAGYALAGRHVHVVTINDYLARRDAEWMGPLLEAIGLTVGWITAESTREDRKAAYGCDVTYASVNEIGFDVLRDQLVTDVDDLVSPHPDVALIDEADSVLVDEALVPLVLAGATHRETPRLEIIKLVGELSAESDYDTDSDSRNVHLTDVGARKVEKALGGIDLYSEEHVGTTLTEVNVALHAHVLLQRDVHYIVRDDAVHLVNASRGRIAQLQRWPDGLQAAVEAKEGIETTETGEVLDTITVQALINRYATVCGMTGTALAAGEQPAEQAGELYEAMSRLVPRLQAQKPEERPEENPELGPGDYYVDEKARQVYLTEGGHDRAEELLREEGLIGENDSLYDARNINVVHHLNAALRAHTLYERDVHYLIRDNQVVIVDEFTGRAMPGRRWSEGLHQAVF",
    "response#2": "FWRLLGASTDKNLNYSSAEVTAAAAYHKEAADLGDEQLRKACGLLNLNDLADSRDVPQFLAIVREASERSTGLRPFDVQLLGALRMLAGDVIEMATGEGKTLAGAIAAAGYALAGRHVHVVTINDYLARRDAEWMGPLLEAIGLTVGWITAESTREDRKAAYGCDVTYASVNEIGFDVLRDQLVTDVDDLVSPHPDVALIDEADSVLVDEALVPLVLAGATHRETPRLEIIKLVGELSAESDYDTDSDSRNVHLTDVGARKVEKALGGIDLYSEEHVGTTLTEVNVALHAHVLLQRDVHYIVRDDAVHLVNASRGRIAQLQRWPDGLQAAVEAKEGIETTETGEVLDTITVQALINRYATVCGMTGTALAAGEQLL",
    "response#3": "IRSRMITKQVESAQKRVEGNNYDSRKNVLQYDDVMRQQRDVMYGERNQVINQDKSLKWVLMPMIKRTVDRVVSLHTQGEQSDWDLDTLLDFGISAMVSPDKISLDDLKNKSADEIKAFFMELAEDVYAEKQKQLYDPAQMLEFEKVVLLRVVDSHWTDHIDAMDQLRQSIGLRGYGQLNPLVEYQQDGFRMFEEMISDC"
  },
  {
    "reference": "MRVTGIRKNCRRLWRWGIMLLGMLMICSALENLWVTVYYGVPVWKDATTTLFCASDAKGYETEVHNVWATHACVPTDPNPQELVLGNVTENFNMWKNDMVDQMHEDVISLWDQSLKPCVKLTPLCVTLKCKDVSINNGNVSSSNGSTSHNNSSIDNETLNEGMKEMKNCSFNATTVLRDKKQKVHALFYRLDIVPLNESNKNSRKYRLINCNTSAITQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGTGPCRNVSTVLCTHGIKPVVSTQLLLNGSLAEEDIIIRSENLTNNAKTIIVHLNESVEIVCTRPGNNTRKGIGIGPGQTFYATGEIIGDIRKAHCNISKDNWTETLYRVSRKLKHFWNKTIIFAPHSGGDLEITTHSFNCRGEFFYCNTSGLFNGTLNDTYMHNSTKSNETITIPCKIRQIVRMWQQVGQAMYAPPIEGNITCKSNITGLLLVRDGGNINRTNETFRPEGGDMRDNWRSELYKYKVVEIKPLGIAPTKAKRRVVEREKRAVGLGAVFLGFLGIAGSTMGAASITLTVQARQLLSGIVQQQNNLLKAIEAQQHLLQLTVWGIKQLQARVLAIERYLKDQQLLGIWGCSGKLICTTAVAWNSSWSNKSLGEIWDNMTWMQWDKEINNYTSIIYSLLEDSQIQQEKNEKDLLALDSWNNLWNWFDITSWLWYIRLFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTLTPNPIPGGPDRLGRIEEEGGEQDRDRSIRLVSGFLALAWD",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm..2. The protein should have a specific function in mitigation of host antiviral defense response and should be localized to host cell endosome membrane in order to carry out this function efficiently.3. The presence of Retroviral envelope protein GP41-like, Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWRDADTTLFCASDAKAHETEVHNVWATHACVPTDPNPQEIPLDNVTENFNMWKNNMVEQMQEDVISLWDQSLKPCIKLTPLCVTLNCTNTILTNTTLTNSTTNGNSSIGNVTDEVKNCTFNMTTEIRDKQQKVRAFFYKLDIEPMGGNDSSEYRLISCNTSVIKQACPKISFDPIPIHYCTPAGYAILKCNDKKFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIISSENLTNNAKNIIVHLNKSVIINCTRPSTNTRKSTRIGPGQVFYRTGDITGDIRKTYCEINGTVWNETLKQVAKKLKEHFNKTIVFQPPSGGDPETTMHHFNCRGEFFYCNTTKLFNGTENGTMEGLNTTIILPCRIKQIVRMWQEVGQAMYNPPISGNITCISSITGILLTRDGGNNATNETFRPGGGNIKDNWRSELYKYKVVQIEPLGIAPTRAKRRVVDREKR",
    "response#2": "LWVTVYYGVPVWKEATTTLFCASDAKAYETEKHNVWATHACVPTDPSPQEVALENVTETFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDNLNLTCPNNNTCSNNTNYNMTANGEIKNCSFNVTTGIRDRVTKEHALFYKLDVVAINEGSGNTTTGKYRMINCNTSVITQACPKVSFEPIPIHFCAPAGFAILKCNDKKFNGTGECRNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVIRSANLSNNAKTIIVQLNKSVKINCTRPNKKIRRRIHIGPGRAFYGTDIIGDIRQAHCNISGKDWNDTLKQIVIKLKEKFENKTIVFTQSSGGDPEIVMHSFNCGGEFFYCNTTQLFNSTWNGTDNWNGTESKSTITLPCRIKQIINLWQEVGRAMYAPPIQGQIRCSSNITGLLLVRDGGSNNSSSDTETFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTHAKRRVVQREKR",
    "response#3": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQFQARVLAIERYLEDQQFLGIWGCFGKFICFFVVPWNSSWSNKSHKDIWDNLPWTRWDRKICIFPAPISRLLEDSQTQPEKNGKDFLELDKWQNLWNWFSLTNWLWYFRIFLMIVGGLIGLRIVFAVLSLVNKVRQGYSPLSFQTY"
  },
  {
    "reference": "MKSIFILVLGNTEVSLIPGISVAGATPELTKLTPPADAEYLFYEKPRIIDAIPVTPEGHPTPAIITKAARELANFPILVVRGGTYLAPLVPHVHISSAVGRDFRREPALPEFGEIVRMAKLLGEELNRTEIEELVIGESTPGGTTTAQAVLWAMGYDARTSSASPENPQSLKEQVIAEGFQRAGIERGQLKDNPLEALRQFGDPMMATVVGLALGFRKNVVLAGGTQMLAVSALLKALEEDMSRFMIATTKWVVRDKSATFIETAKEIGIITYAADLDFSKSEFKGLRDYENGYVKEGVGAGGATWLAIKAGFSPEEVSAKVEELYRRLMGMKSP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity. The designed protein sequence is ",
    "response#1": "ISTKASKVRLAAGDSPFFSFGIRRMHPAISPMIDRSAYIGGADGVSGILGAKLIDQDPVGTMPHALSIMLGDEEAWKLTLENTKNGQKSVLLIDTYMDEKFAAIKIAEMFDKVDYIRLDTPSSRRGNFEALIREVRWELALRGRSDIKIMVSGGLDENTVKKLREAGAEAFGVGTSISSAKPFDFAMR",
    "response#2": "FQVADFGTRRRFSYRTQEEVVHILKRDFPGRFVGTSNVHLAREYDLKPIGTMAHEWLMAHQQLGPRLVDSQQAALDCWVREYRGQLGIALTDCITMDAFLDDFDLYFAKLFDGLRHDSGDPLAWAEKAIAHYRRLGIDPLSKTLVFSDGLDMPKALQLFRALRGKINVSFGIGTNLTCDIPGVEPMNIVLKMTACNGHPVAKISDAPGKTQCRDENFVAYLRHVFNVY",
    "response#3": "ISTKASKVRLAAGDSPFFSFGIRRMHPAISPMIDRSAYIGGADGVSGILGAKLIDQDPVGTMPHALSIMLGDEEAWKLTLENTKNGQKSVLLIDTYMDEKFAAIKIAEMFDKVDYIRLDTPSSRRGNFEALIREVRWELALRGRSDIKIMVSGGLDENTVKKLREAGAEAFGVGTSISSAKPFDFAMQ"
  },
  {
    "reference": "MEFKNQVYGIIAEVCQDDVVKENPEIAIFDEGLLDSFGTVELLMAIESQLGITVPITEFDRDVWDTPNHIAEQLAEMK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein have Carrier domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "IYSGMGSQWASMAKDMMQVEVFRNSIYRCAEALRPEGVDLIDILTKSTEEKFENILNSFISIAAVQVALTDVLTHLGITPDGMVGHSVGELGCAYADGCFTPEQTVLASYWRGRSILDTDLVSGQMAAVGLSWEECLRRLPKDVIPACHNSVDSVTISGPVPSVTKVVTELNAEGVFAKAVKSSGIAFHSKYIADAAPKLRKSLDKIIPTPKNRTKRWISTSIPESSWDTPIAQQSSAAYHDDIAIIGVGLRLPGEESGGGVVGELLNDSPQNFWNNLKKGFNGISKTNERWSENYSKLGEISNGYAGVLPLEELKSFDPLFFGISPSEASTIDPQQRLLLKTTWESLEDAGIDHQSIRGSDCSVFIGSSTTEYRESIVNINEQYLNIFGTYAHGIANRISYCFDLRGESVTLDTACASSGTAISMGAQCIKNGKSKLSIVGGVNLIVDTTNIKAFSYLNMLSTSGVCRPFDIGADGLLRGEGVGTIVLKSLKT",
    "response#2": "IDCGCSSNYTDPRTGLGWVSDSEIIKQGKPVTLANTNWNSMQYRRRRDFPTDNKKYCYRLSTKERRRYIVRTTFLYGGLGSEEAYPKFQLYLDATKWATVTIQEVSRVYVEELIVRATSSYVDVCVCCAITGSPFMSTLELRPLNLSMYATDYEDNFFLKVAARVNFGAPNMDALRYPDDPYDRIWESDINKRPNYLVGVAPGTTRINTSKTINTLTREYPPMKVMQTAVVGTQGLISYRLNLEDFPANARAYAYFAEIEELGANETRKFKLVQPYFPDYSNAVVNIAENANGSYTLYEPSYMNVTLDFVLTFSFGKTKDSTQGPLLNAIEISC",
    "response#3": "VFKQIIDGLIDCEKVFENENFIAIKDRFPQAPVHLLIIPKKPIPRFQDIPGDEMILMAEAGKIVQELAAEFGIADGYRVVINNGAEGGQAVFHLHIHLLGGRPLGAII"
  },
  {
    "reference": "MDQSLTQDRPLAAVFRRLGSELMPPVVETFDRSKTIFFPGDPAERVYFLLKGAVKLSRVYEAGEEITVALLRENSVFGVLSLVTGQRSDRFYHAVAFTPVELLSAPIEQVEQALKEHPDLSLLMLQGLSSRILQTEMMIETLAHRDMGSRLVSFLLILCRDFGVPAPDGIRIDLKLSHQAIAEAIGSTRVTVTRLLGDLREGNMISITKKKITVHNPVALSQQFT",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The H-T-H motif domain within the designed protein should exhibit high affinity and specificity towards its target DNA sequence.2. A protein that localizes to cytosol and can perform DNA binding, DNA-binding transcription factor activity with high specificity and efficiency.3. For general function, the protein need meet that Transcriptional activator of genes subject to nitrogen control.; Required for full expression of proteins subject to ammonium repression The designed protein sequence is ",
    "response#1": "MVGKSPAMRQIMDIIRQVSRWDTTVLVRGESGTGKELIANAIHHNSPRAAAAFVKFNCAALPDNLLESELFGHEKGAFTGAVRQRKGRFELADGGTLFLDEIGESSASFQAKLLRILQEGEMERVGGDETLRVNVRIIAATNRHLEEEVRLGHFREDLYYRLNVMPIALPPLRERQEDIAELAHFLVRKIAHSQGRTLRISDGAIRLLMEYSWPGNVRELENCLERSAERQVNIVVNNLLYSPQAVASLLSWLNARYPFTQFHFSRQIYMGVWDSLLYEGFSLAIGVTGTEPLANTFMLDPLGSVQWRFVMSADHPLAHVSGPLTEAQLRRFPAINIEDSARTLTKRVAWRLPGQKEIIVPDMETKIAAHLAGVGIGFVPQPLCQTLIDKNELVSCTIPTMRPPSPLSLAWHKFGGGKAVEDIVKLFTRR",
    "response#2": "MVGKSPAMRQIMDIIRQVSRWDTTVLVRGESGTGKELIANAIHHNSPRAAAAFVKFNCAALPDNLLESELFGHEKGAFTGAVRQRKGRFELADGGTLFLDEIGESSASFQAKLLRILQEGEMERVGGDETLRVNVRIIAATNRHLEEEVRLGHFREDLYYRLNVMPGVCARCDSEELATLEQIKYYRSYQAGQTVIWSGDKMDFVASVVTGIATLTQTMEDGRRQMVGLLLPSDFVGRPGRQTVAYDVTATTDLLMCCFRRKPFEEMMQKTPHVGQRLLEMTLDEL",
    "response#3": "MVGKSPAMRQIMDIIRQVSRWDTTVLVRGESGTGKELIANAIHHNSPRAAAAFVKFNCAALPDNLLESELFGHEKGAFTGAVRQRKGRFELADGGTLFLDEIGESSASFQAKLLRILQEGEMERVGGDETLRVNVRIIAATNRHLEEEVRLGHFREDLYYRLNVMPIALPPLRERQEDIAELAHFLVRKIAHSQGRTLRISDGAIRLLMEYSWPGNVRELENCLERSAGRRLLRIAASSAFAEHAAPGLIELFSSRADDLSVELSVHPTSRFRELICSRAVDIAIGPASESSIGSDGSIFLRPFLKYQIITVVAPNSPLAAGIPMPALLRHQQWMLGPSAGSVDGEIATMLRGLAIPESQQRIFQSDAAALEEVMRVGGATLAIGFAVAKDLAAGRLVHVTGPGLDRAGEWCVATLAPSARQPAVSELVGFQSDRRFRIVLSDYVTLVFFEKVVARAAQEAPGISFDCLPLADDFEELLRRGDIDFLIMPELFMSMHPHAALFEDKFVCVGCRTNEQLSEPFTFERYMSMGHVAVKFGNTRRPTIEEWYLLEHGLKRRIEVVVQGFSMIPPMLSGTERIGTMPLRLAQHFAKTIPLRIVELPLPIPPLAEAVQWPALHNSDPASLWMRELLLQEL"
  },
  {
    "reference": "MSYAYTADQIRAAEQPLLEAQDFPDQLMQQAASAVFEVARALVLDDDHPAVLVLAGKGGNGGDGLYAGAQLAMQGVAVSAFLTAGEAHQPALTVFEQAGGTVLTEAPTDEECARYQLVIDAMTGIGGSGGIADEAYRIVEAARAGQVPVLAVDVPSGVDADTGRAHPQHVTADVTVTFGGFRLAHGLAPECGLQVLAEIGTPPLSNILQRQNSPYVLHRATLPDTSWLEPLQSLQTLRPPAITSVEPSATDDKYSGGVVGIRAGSGTYPGAALLSVRGAVDATSAMVRYAGPQALEVVRAHPEVVVTETIQDAGRVQAWVFGPGAGTDEDAAAELEYLLETDLPVLIDADGLTLLAEHENLRGLVVKRKATTVLTPHDGEFTRLREACGLDSAGRLAETETLAQHLQATVVRKGRSTIIAGGSRTHIIDAGNSWAATPGSGDVLSGIAGAHLAADTPDAVVQAVAIHAAAAKLSATTPYGEGPTSATRIADAVPAASAQLTGA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with NADPHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding, metal ion binding, NADHX epimerase activity is needed to enhance nicotinamide nucleotide metabolic process in a specific context.2. The desired protein should be able to perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate3. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.4. The designed protein should have a high specificity for K(+), (6S)-NADPHX, AMP binding, with minimal off-target effects.5. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.6. The designed protein must have at least one YjeF N-terminal domain. The designed protein sequence is ",
    "response#1": "MKQYDEYLLNHGYQINELVDKASDCLIKHMHGQRYCLLCGPGNNGADGLSLAIKLFLQGKQVTVYIFEDQNHLSQANRYYLDQCYALNLEVILLNEDILDEVILHMKDCDIIVDAMFGNGLNSSPRGLYQMVIEQINQLYDQEIIAVDIPTGLNCDNGKPYQSVVCATQTITLTALKNGFLNPDSTSFTGQVIVEELDVEDVSEVLVFGGDTSMMGAPVLSALASMRGGAGLASIVMPEQLRSAYTFSQPEIMIKTYCFEALPETIGELDEKELSIFGPGLGKKTPAYEQVIEHIMQQPRPLIIDADGLHYAKRWRSELLGKPYVVITPHGGEFNRFFDLSSRQDNDVIATVETLMQDTEAILVLKGPVTLVVRSGRACWIPFGSSVLAKAGTGDVLSGLIAARAAVNTSLFDATCEAVALHAQAGKCIERRHGREGLVASDLEAHKNQFGRIGIVAGSRGFAGAATMCAWGALRAGAGLVEVFVPENIYEVVAASAPFESMVKPVTSYRDLLDEKIDVWGIGPGLGKENAREIIDLVQRTKNAMVVDADALNLISREVSILKNCRGPRLLTPHPGEMKRLAGDRKISRAKLAKNFTAEFPVTLLLKGSRTVVAENGKRISYNTTGNPGMATGGMGDVLTGVCASLIAQNLSPFDAARVGAWVCGRAAELE",
    "response#2": "MVVLDGQQVVSFEKSCGVAIDELICKAGKVIGDTIFNMFPRQPVIVISGPGNNGRDGIVAAKMLKAHGWPVVLMLYNCTTHIDEDWVVPLTYDNIVNSQSCLIVDALFGIGLSRYIPEDLSCIFSYINNSNNKIVVAVDIPSGINCNTGQVMGCAIRADVTITFSALKIGHVLFPGSSYSGKVRVVDIGIGIDYSKSSHKGDNGRVLIVGGGPFAGAPALSALASLRTGADWVTVAAPRNVASIIASISPNLIVQPLSGSGSGSILTEDDVSLVSSLMQMHDVLVIGMGLGAAEETKRAVKLIIEDEAVKKVVVDADGLYGLDLPLKVSKLKSKLKSKQVIITPHAGEFSKMDAQRPPPDGEERLGFIQEFSLRNRVVTLLKAAHDVISDGKRIKVNATGNAGMTVGGTGDVLSGITGALFATSEDAFEVATAAAFISGSAGDAAFRDKGLSLLATDVVEAIEGHKYLRGHAVVASGPIYSTGAARLAARGALRAGAGLVTVASPRDALAVNAAQLTAIMVREADDARGLAALLADTRKNAVLIGPGHGVGEGTRAMVLAALAAGPRVVLDADALTSFADDPETLFKAIHASEAPVVMTPHEGEFARLFGDLIAEPKVERTRKAAARAGATVLLKGADTVIAAADGQAAINGTASPWLATAGSGDVLGGIVLGLLAQGMTGFLGASAAVWMHGEAAKTFGPGLIAEDLPEMLPAVVTVASPRDALAVNAAQLTAIMVREADDARGLAALLADTRKNAVLIGPGHGVGEGTRAMVLAALAAGPRVVLDADALTSFADDPETLFKAIHASEAPVVMTPHEGEFARLFGDLIAEPKVERTRKAAARAGATVLLKGADTVIAAADGQAAINGTASPWLATAGSGDVLGGIVLGLLAQGMTGFLGASAAVWMHGEAAKTFGPGLIAEDLP",
    "response#3": "MKQYDEYLLNHGYQINELVDKASDCLIKHMHGQRYCLLCGPGNNGADGLSLAIKLFLQGKQVTVYIFEDQNHLSQANRYYLDQCYALNLEVILLNEDILDEVILHMKDCDIIVDAMFGNGLNSSPRGLYQMVIEQINQLYDQEIIAVDIPTGLNCDNGKPYQSVVCATQTITLTALKNGFLNPDSTSFTGQVIVEELDVEDVSELLITGCHEYKGASLLSAKSCVYSGSGVVTVMSEQAVIDALTVYCPEVTTQLRPAVMRKEDFLKYQAILIGCGLGQSIDSYRHVIDVFSMSHQPLVVDADALTILSSNLDLLKNQERDIILTPHMGEFRRLCEFDEHSDMLFVARNFALKHHVILVLKGPYTIVTDGQESYRIFAGNKAMASGGMGDTLAGIITSLLGQGYGGLQAAMLGVYIHGRAGDCLANDAYTIIPSKLIDVIGPGDVRLVLRRREKSSHKGDNGRVLIVGGGPFAGAPALSALASLRTGADWVTVAAPRNVASIIASISPNLIVQPLSGSGSGSILTEDDVSLVSSLMQMHDVLVIGMGLGAAEETKRAVKLIIEDEAVKKVVVDADGLYGLDLPLKVSKLKSKLKSKQVIITPHAGEFSKMDAQRPPPDGEERLGFIQEFSLRNRVVTLLKAAHDVISDGKRIKVNATGNAGMTVGGTGDVLSGITGALFATSEDAFEVATAAAFISGSAGDAAFRDKGLSLLATDVVEAIPGILRAL"
  },
  {
    "reference": "MASSAHTKHVFVTGGVASSLGKGLTASSLGNLLKSRGLRVTMQKLDPYLNVDPGTMNPFQHGEVFVTDDGAETDLDIGHYERFLDTELNQIANVTTGQVYSTVIAKERRGDYLGDTVQVIPHITNEIKDRVRAMGGDDVDVVITEIGGTVGDIESLPFLESARQVRHDIGRDNCFFLHVSLVPYLGPSGELKTKPTQHSVAALRSIGIQPDAIVLRSDREVPQSIKDKISLMCDVDKEAVINCADAPSIYDIPKVIHSEGLDAYVVRRLNLPFRDVEWTVWDDLLRRVHHPSEEVTVALVGKYIDLPDAYLSVGEALRAGGFAHEAKVKIEWIASDECATPAGAAKHLSDVDAICVPGGFGIRGIEGKLGALTYARTHGIPTLGLCLGLQCMVIEYARSEAGLEKAASTEFDADAPEPVIATMEEQKSFVEGAGDLGGTMRLGTYPAHLKEGSVVREAYGAAEIEERHRHRYEVNNAYRDQLEAAGLVFSGTSPDNNLVEFAELPKDVHPYYVSTQAHPELKSRPTKPHPLFAGLIGAAIERQKELRFPIDEELHRKDEQA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The L-glutamine, CTP binding site should be stable and able to withstand changes in environmental conditions.2. The designed protein must possess metal ion binding to support 'de novo' CTP biosynthetic process, glutamine metabolic process under certain conditions. The designed protein sequence is ",
    "response#1": "LPDAYLSVVESLKHAGFHHGAAVEIDWIQAEDVEGLLAAGRLADLDGIVIPGGFGARGVEGKIAAARYAREELIPCLGLCLGMQTMTIDYARHVLGLSDANSTEFDPTTHHPVIDLMLDQRDITDKGGTMRLGTYFAVLAPGTKVAEAYGEMVVSERHRHRYEFNSAYQARLEEAGLVCSGASPDKRLVEFVEDTDHPFWIGTQAHPEFKSRPDRPHPLFRDLLA",
    "response#2": "VVIGLVGKYVELQDAYKSILEAFVHAGAVNECKVEVRNIHSEXKLKNNVRTLFSGLDGILVAPGFGSRGIEGKILAIQYAREXKIPFFGICLGMQCSVIEFARNVLGYPTANSTEMDKHTPYPVIDIMEDQKNIIQMGGTMRLGAYECELKAGSKIHEVYGQEFISERHRHRWEFNNTFLEAFEQAGMKATGRNPKTGLVEVVEIPDRPFFVGSQYHPELKSTVEHPHPLFSGFIHH",
    "response#3": "LADSYISVIEAVKHAAWRLGYKPEIDFLSSQDLEKRGPKEFEEVSGIIVPGGFGQMGVEGKINAIKFARENDLPFLGLCFGMQLAVVEFARNVCKLKGANSTEVDLHTPHPVVDLLPWQKKLLKQNLYGATMRLGEHKVKIVSKSLAARLYGKRYVYERFRHRYEVNPKYVKRLERNGLFFSGTTPDGKIMQIVELPDHRFFLGTQFHPEFTSRPLNPHPLFLGFVAMGK"
  },
  {
    "reference": "AFCHIFLLFSPIFLSSFSSTRRFLPSKQEDDDYEDVPINKTWVLSPKVYESDVTLILNKLLQGYDNKLRPDIGVRPTVIETAVYVNSIGPVDPINMEYTIDIFFAQTWYDSRLKFNSSMKLLMLNSNMVGKIWIPDTFFRNSRKSDAHWITTPNRLLRLWSNGRVMYTLRLTINAECYLKLHNFPMDEHSCPLEFSSYGYPRNEIMYRWQRRAVEVADQRYWRLYQFAFVGMRNTSDVAHTQSGEYVIMTIFFDLSRRMGYFTIQTYIPCSMIVVLSWVSFWINKDAVPARTSLGITTVLTMTTLSTISRKSLPKVSYITAMDLFVSVCFIFTFAALMEYGTLHYFTSNRQTKKTKNKGSSMANLRPGTSLLQMNNIVPYHEEDSFAYECLDGKDCASFFCCFDDCRTGAWRENRMHVHVSKIDSYSRIFFPTAFGLFNLVYWIGYLYL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization.2. The protein should be designed to localize specifically to postsynaptic membrane in order to perform its role in synaptic transmission, GABAergic, ion transmembrane transport, gamma-aminobutyric acid signaling pathway more effectively. The designed protein sequence is ",
    "response#1": "LTFRLTVQAECPMHLEDFPMDAHSCPLKFGSYAYTTSEVTYIWTYNASDSVEVAPDGSRLNQYDLLGQSVGKETIKSSTGEYTVMTAHFHLKRKR",
    "response#2": "TRILDRLLDGYDNRLRPGFGGPVTEVKTDIYVTSFGPVSDVEMEYTMDVFFRQTWVDKRLRYDGPIEILRLNNLMVTKVWTPDTFFRNGKKSVAHNMTAPNKLFRIMRNGTILYTMRLTISAECPMRLVDFPMDGHACPLKFGSYAYPKSEMIYTWTKGPQHSVEVPEESSSLVQYDLLGHTASTDTVKSITGEYIVMTVHFHLRRKY",
    "response#3": "LASKIWTPDTFFHNGKKSVAHNMTTPNKLLRLVDNGTLLYTMRLTIHAECPMHLEDFPMDVHACPLKFGSYAYTKTEVIYTWTLGKVEVAEGGSRLNQYDLLGHNVGRDSIESSTGTYIVMTTYFHLKRKR"
  },
  {
    "reference": "MKNEIIERFTSYVKVDTQSDENNENCPSTPGQLTLANMLADELKSIGMTDITVDDNGYVMATLPANTDKDVPTVGFLAHVDTATDFTGKNVNPQIIESYDGHDIVLNDKLNVVLSPEQFPELSRYKGQTLITTDGTTLLGADNKAGIAEIMTAMAYLIKHPDIKHGTIRVAFTPDEEIGRGPHKFDVGRFNAAFAYTIDGGPLGELQYESFNAAAAKLTCYGKSVHPGTAKNKMVNASKIAMEFHHALPKEEAPEYTEGYEGFYHLLSMNGDVSEAALSYIIRDFDRDRFAERKEYMQKTADALAAKYGNESIKLELRDQYYNMREKIEPVKEIVDVAYQAMKNLDIDPIIEPIRGGTDGSQLSYMGLPTPNIFTGGQNFHGKYEYISVDHMEKAVNVIVEIARLFEERA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be involved in proteolysis by localizing to cytoplasm and exhibiting zinc ion binding, tripeptide aminopeptidase activity.2. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity.3. The protein must exhibit the following characteristics: Cleaves the N-terminal amino acid of tripeptides..4. The designed protein is the optimization of the active site to achieve high catalytic efficiency for Release of the N-terminal residue from a tripeptide.. The designed protein sequence is ",
    "response#1": "AGLLYQGEPGALNESISDIMGAMADRDDWEIGEDVYTPGIAGDSLRSLEDPSKQGNPDHYSNRYTGTEDYGGVHINSSIHNKAAYLLAEGGVHHGVQVEGIGREASEQIYYRALTYYVTASTDFSMMKQAAIEAANDLYGEGSKQSASVEKAYEAVGA",
    "response#2": "AGLLYQGEPGALNESISDIMGAMADRDDWEIGEDVYTPGIAGDSLRSLEDPSKQGNPDHYSNRYTGTEDYGGVHINSSIHNKAAYLLAEGGVHHGVQVEGIGREASEQIYYRALTYYVTASTDFSMMKQAAIEAANDLYGEGSKQSASVEKAYEAVGQ",
    "response#3": "GTGIGVSGDEKSFDVTEQNGRFYLADETRGKGINTFDAKNLNETLFTLLSQLIGYTGKEIVSGTSVFNEPAAVDAHANAQAVYDYYSKTFGRDSFDQNGARITSTVHVGKQWNNAAWNGVQMVYGDGDGSKFKPLSGSLDIVAHEITHAVTF"
  },
  {
    "reference": "MSLKDKKIILAVTGSIAAYKTPQLVRLLVKAGAEVQVVMTEAAKDFVTPLALATVSTKEVFYAVSNGAHWNNHVHLGRWADVLLVAPCTANTLAKMAQGLCDNMVQAIYLSAACPVVFAPAMDEDMWLHPATRANVQQLQSYGHHLIPVESGPLASGLTGPGRMAEPETIAAWLEHFFAPEQQPVAIPPNTKALITAGPTYEAIDPVRFIGNFSTGKMGIALAEALAERGIPVDLVLGPSKESTQHPLITTHKVVSAREMYDACERLFPEAAISILSAAVADFRPQEAAAEKIKKGSEDTMQLTLVKNPDILAHLGAIKNAGQTVVGFALETNNELEHALKKLKAKNADMIVLNSLQEAGAGFGHDTNKVTLLSADGTQTALPLQSKKEIAKSIVDCVLALKYNNA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. Include a signal peptide as an essential component of the protein.2. The protein contains novel Flavoprotein domains that confer a unique function or activity.3. The protein should be optimized for activity in Cofactor biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MRIVLGVSAGIAAYKSVHLARLLKEDGHQVDVIPTSSSLEFVGRATWEAISGRPVSTTVFEGVDEVRHVRLGQEADLVVVAPATADLLAKIRAGMADDLMTTTLLATEAPTLLVPAMHTEMWRNAATRENVAGLRARGIEVMEPAVGRLTGKDSGPGRMPEPSEIYARVQQALGG",
    "response#2": "MRIVLGVSAGIAAYKSVHLARLLKEDGHQVDVIPTSSSLEFVGRATWEAISGRPVSTTVFEGVDEVRHVRLGQEADLVVVAPATADLLAKIRAGMADDLMTTTLLATEAPTLLVPAMHTEMWRNAATRENVAGLRARGIEVMEPAVGRLTGKDSGPGRMPEPSEIYARVQQVLTAG",
    "response#3": "LENRKVLITGGSTAESLDPIRILTNRASGKTGRELALEAYRSGADVTLVHRDRLRLVGIREVFAESAAEMTEAVLSELDTGYDVLISSAAIADYTVEPSPEKIKSGGEFALKLKTTRKLIRECREKYPELVIIGFKAETGVGREELLKRATATLEGTKLDMIAANDVGKGGMGTEENELYC"
  },
  {
    "reference": "MRRWVLAIAILAAAVCFFLGAQAQEVRQGHQTERISGSAGDVLEDDPVGRLKVYVYDLPSKYNKKLLKKDPRCLNHMFAAEIFMHRFLLSSAVRTFNPEEADWFYTPVYTTCDLTPSGLPLPFKSPRMMRSAIELIATNWPYWNRSEGADHFFVTPHDFGACFHYQEEKAIGRGILPLLQRATLVQTFGQKNHVCLKDGSITIPPYAPPQKMQAHLIPPDTPRSIFVYFRGLFYDTSNDPEGGYYARGARASVWENFKNNPLFDISTDHPPTYYEDMQRSVFCLCPLGWAPWSPRLVEAVVFGCIPVIIADDIVLPFADAIPWEEIGVFVAEEDVPKLDSILTSIPTDVILRKQRLLANPSMKQAMLFPQPAQAGDAFHQILNGLARKLPHGENVFLKPGERALNWTAGPVGDLKPW",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be designed to localize specifically to Golgi membrane in order to perform its role in cell wall organization more effectively.2. The Lumenal topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "VRTGMLSGGSSIITTSYEGTVAAYKFNGEKLWENELSGFMNHDIWVQDINGDGLVEIFAANADGNVYCINSDGSLKWTFGLNEVPMNSVTVISDADKKYVVAGGYDKNLYYISTNGELLR",
    "response#2": "VRTGMLSGGSSIITTSYEGTVAAYKFNGEKLWENELSGFMNHDIWVQDINGDGLVEIFAANADGNVYCINSDGSLKWTFGLNEVPMNSVTVISDADKKYVVAGGYDKNLYYISTNGELLG",
    "response#3": "VRTGMLSGGSSIITTSYEGTVAAYKFNGEKLWENELSGFMNHDIWVQDINGDGLVEIFAANADGNVYCINSDGSLKWTFGLNEVPMNSVTVISDADKKYVVAGGYDKNLYYISTNGELLF"
  },
  {
    "reference": "MTTKRIAVIGAGPTGICSIKCLLEEGMEPVCFERTNHIGGLWRYHDEDIEGLASVMKTTVINSSKEMTAISDFPPPEDAPNYMHNKQIYNLLKTYAETFDIEKYVHFHKEVVEVDMAENYEETGRLVVVVRDTETQETSKDIYDGVMVCIGHHVYPNIPTFPGTEKFKGKIMHTHSLKKIDEFEDQVVVVVGVGNSGMDAAVEISTVAKQVYLSSRKGTWVLPRVGPKGFPLDVTLTNRFTKILMRILPYFLVCWFSEKVLNYTFDHEIYSLKPKHRIWSQHPTVSDTLPLKILTGRVLVRKNIRKFVENGVIFEGEDDVTECDSVILATGYRIHFPFLNDKLATIKNNEVHLYKYIYPPHLKHPTLAIVGLIQSVGGGFPIGEAQCRWISLVFKEKMKLPSREEMEADIKSKREFNEKRYVKSERHTIQVDYIDYLDEIYTMIGAKPNLFKMFFTDPVLFFTLFFGPSLPYQYRLQGPHAWPGARKAILEWKRRVIKPLKKDYNSQPYLAKLNVLKYVFIMLIICVILFLGSGLVAKTEI",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have flavin adenine dinucleotide binding, N,N-dimethylaniline monooxygenase activity, NAD(P)H oxidase H2O2-forming activity, hypotaurine dehydrogenase activity, NADP binding within endoplasmic reticulum membrane to regulate lipid metabolic process.2. The desired protein should be able to perform H(+) + hexan-3-one + NADPH + O2 = ethyl butanoate + H2O +   NADP(+)3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "MXKLLFITGTDTGVGKTVASCALLRAFRAAGVDAVGMKPAQSGPADAADASRLREIAGVAGPDALVTPYRFDTPLSPHLAARREKVAVDLDRIRRSFDSLSRRHEFVLVEGAGGLAVPLREGYTLADYARRYGYPVLLVADSGLGTINHTLLTVEAVRQLGLKLAGVIVNRMAPGPDTVAETNPSVIRRYSRLPVLARLPWVETLGPEALDRVARDFERQVDLPAILALAGDA",
    "response#2": "MSILSGKVALVTGSGRGIGAAIAIKLAELGATVVGCARSPEKLDSLKAEIEAAGGK",
    "response#3": "MSTSS"
  },
  {
    "reference": "MKLNKYIDHTLLKPEASEEQILKLIEEAKVYDFASICVNPTWIEFAAEQLKGSDVKVCVPIGFPLGANTSDVKAFETKDAIQKGAGEVDMVINVGALKSKNYDLVERDIRAVVEAANGTLVKVILETCLLTDEEKVKACQLAQKAGADFVKTSTGFSTGGATVEDVALMRKTVGPDMGVKASGGARSYEDALAFIEAGATRIGTSAGVAIMKGEEASGDY",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be optimized for activity in D-glyceraldehyde 3-phosphate and acetaldehyde from 2- deoxy-alpha-D-ribose 1-phosphate: step 2/2 pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The Schiff-base intermediate with acetaldehyde active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The designed protein should have deoxyribose-phosphate aldolase activity to facilitate carbohydrate catabolic process. The designed protein sequence is ",
    "response#1": "EPTM",
    "response#2": "MPTITVQKHDG",
    "response#3": "MPKIQIL"
  },
  {
    "reference": "MTTAPSGLPGTPLPPARRRERPRWPLLVAGAAVLGLIGYMVLGNANSNLVYYVLPSEYQQDTAKFAGKTLRMGGLAKDVVYNRDTLALKFNMTDGQVAYPVQYQGAVPDMFRNDAVVVMEGQMQQGVFHGKSLLVKHSEEYKAADSKGEGQYSQDDLKKILNDQSTKP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit heme binding. The designed protein sequence is ",
    "response#1": "VSMADLIVLAGCAGVEQAAKAGGHQIEVPFTSGRGDASAEQTDAESFAVMEPVMDGFRNYQARELSTSPEEMLVDRAQLLGLSAPEMTVLVAGLRVLGANHGGSAHGVLTNRPGVLSSDFLTNLLDMGTEWKPSGKGVYEGRDRASGAARWTATRVDLVFGSNSQLRALAERYAQDDAEASFVADFVAAWVKAG",
    "response#2": "VHLTAEDRKEIAAILGKVNVDSLGGQCLARLIVVNPWSRRYFHDFGDLSSCDAICRNPKVLAHGAKVMRSIVEATKHLDNLREYYADLSVTHSLKFYVDPENFKLFSGIVIVCLALTLQTDFSCHKQLAFEKLMKGVSHALGHGC",
    "response#3": "VSMADLIVLAGCAGVEQAAKAGGHQIEVPFTSGRGDASAEQTDAESFAVMEPVMDGFRNYQARELSTSPEEMLVDRAQLLGLSAPEMTVLVAGLRVLGANHGGSAHGVLTNRPGVLSSDFLTNLLDMGTEWKPSGKGVYEGRDRASGAARWTATRVDLVFGSNSQLRALAERYAQDDAEASFVADFVAAWVKR"
  },
  {
    "reference": "MHDLKLLSGRANPALSREIADHLGVPLGKISLGNFPDGEIACKVDEDVRGRDVFLIQPTCPPVNESLMELLVMIDSCLRASAERITAVIPYFGYARQDRKDEGRVPITAKLVANMITRAGADRVLTMDLHAPQIQGFFDVPVDHLYGAPVLNDYFLHDSGIALDDVVIVSPDEGSIKRALGHAKRLGGTVAIIDKRRVSATKTVQANILGGPIDGKVALMFDDMISTAGSIKGAARVLHDHGVKEIHVATSHAVLCGSAMDNLREADLTSLVCTNSIPLRDDQKLPQTKILSVAPLLGGAIKAIHRNESISKLFR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that is capable of catalyzing reactions within the 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "AEPAVLQWIRENITEWRNCIIVSPDAGGAKRVTSIADRLNVEFALIHKERKKANEVDRMVLVGDVKDRVAILVDDMADTCGTICHAADKLLSAGATKVYAILTHGIFSGPAISRINNAAFEAVVVTNA",
    "response#2": "SPILLEDMLQQNLENPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAVDNIRNSVIDEVIVCDPD",
    "response#3": "AEPAVLQWIRENITEWRNCIIVSPDAGGAKRVTSIADRLNVEFALIHKERKKANEVDRMVLVGDVKDRVAILVDDMADTCGTICHAADKLLSAGATKVYAILTHGIFSGPAISRINNAAFEAVVVTNGN"
  },
  {
    "reference": "MAPKTKALKLKRALKLGKNRGTPLAGEDPIVLLRVQILGCKDLLAKDRGGTSDPFINISLLTARFQTPVIKKTLAPTWADGTATYNIPLFLSVAHELGVLELVVWDKDLLKKDYLGEAGISLEDWFGPDSTRSRALGFDDQGNMPFSLPLQSSRPGTTASGTITLKLGFVQAPGTNNLLELDEIYSELVRRARPSLVSAPPTEGVGTVRSSHYVPYEDDGGISSDSDSDYSNEEEDEDEDEEEFVDAMQTPPKTPGTPAAAPDVEITPASPSSLTQTPNTPTPAMRPSPTPRSPSFMRLKFPRRNTGNSGTSSPTSTVDEDQPKEDVKEKKKRFRKSFPSPVSVGRKRKGKEFKLPGPNDIVGIVMLEIKSAEDLPKLSNMTRTGWDMDPFVVISFGKKVFRTRVIRHSLNPVWDERLLFHVRKHETTFGLHFTVSDWDKLSSNDYIGDSSFSVQELLEDAEKCDEETGLYPISSNPETLSDKMKEFKLDISTGKESVWAKRHKPVLTVRAKYQPYDALRQKFWQKYLTQYDTDDTGCVSHLELTSMLDSLGSTLTKTTVDAFWSTYGKNPATDELTIPEAIQCLEAELMRPDSEKRTIAENETGMDTSVSATPVLSVSGSRGEELPLQGLDFSGPPMTARAGVGAGEGADVVPPPPVPTEPAQVPLDRRNPSYSSSSDADLDEDVQTTVTRKRMSRFKKKGKKGTASSGGSSGGNSGSGSSPPSSESSVDPVERVINVKNCPMCHRPRMSSKAEVDIVTHLAVCASS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should exhibit Polar residues compositional bias.2. The designed protein must have at least one C2 domain.3. A protein with phosphatidylserine decarboxylase activity functions is required to enable protein autoprocessing.4. For general function, the protein need meet that Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer) The designed protein sequence is ",
    "response#1": "QSNSRAVSPKRKEEQGDHKGFNLVLRVQVLEGRNLAPKDKNGTSDPYLVITLGDAREATSVIDKTLNPKWNQTFDLPISGISSLLLEGVCWDKDRFSKDYMGEFDVALEDIFTNGQTAQEPKWH",
    "response#2": "IIKANVIKGINITINNSGCNPMCCLFYNGKVLTTSTFKNTSDPIWDTQFKIKLPVLPESDTLRFLIFDKLSSNNSATTTSITNNYNNKNNNNNGAVDGDTANSNIGSNLVPPNVNGTRRHRTNSTASLNSISSNSRSPFR",
    "response#3": "MSASKGDSASPDGRADKGLVLNVNILRARNLAAKDRGGTSDPYLVLTLGEAKHITHAVSKDLNPEWNEQAHLPVAGVESLLLDVCCWDKDRFGKDYLGEFDLALEEIFNGSHTELEPKWY"
  },
  {
    "reference": "MERIYLDHAATSPMDERVLEQMIPHFSGSFGNPSSIHSFGRESRKWVDEARAQIAAEIGAAEQEIIFTSGGTEADNLAIMGTALARKDLGRHIITTKIEHHAVLHTCEKLEGDGFDITYLDVDQNGRVSAKQVKEALRDDTILVTVMYGNNEVGTVQPIEEIGELLKEHKAYFHTDAVQAFGLLPIDVKNSHIDLLSVSGHKLNGPKGTGFLYASKDVKLSPLLFGGEQERKRRAGTENVPGIVGLKEAIKLSSEERDEKNEKYQSFKAIFADTLRDAGVAFEVNGDKEHSLPHVLNLYFPGVSVEALLVNLDMAGVAVSSGSACTAGSVLPSHVLTAMFGEESDRLTSSIRISFGLGNTAEQVKTAAKHVADVVKRLT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit iron-sulfur cluster binding, cysteine desulfurase activity, metal ion binding. The designed protein sequence is ",
    "response#1": "MNTSSSFKALASPPLISTSRPTTKSFPNPRFTSRFSPKPITCMRDSLNLGSNPKAPSPFSLATVSVDAPLGTKTSDKLRILVSEFRSLTEPIDRVKRLLNYAATLAPLDESARISENRVTGCTTQVWLEIKMDEFGRMRFKADSDSEISKGFCSCLIWILDGAKPEEVMGVRSEDLSEMNVGVHGKEQSRVNTWHNVLMSMQKRTMTLVATDVAHR",
    "response#2": "MNTSSSFKALASPPLISTSRPTTKSFPNPRFTSRFSPKPITCMRDSLNLGSNPKAPSPFSLATVSVDAPLGTKTSDKLRILVSEFRSLTEPIDRVKRLLNYAATLAPLDESARISENRVTGCTTQVWLEIKMDEFGRMRFKADSDSEISKGFCSCLIWILDGAKPEEVMGVRSEDLSEMNVGVHGKEQSRVNTWHNVLMSMQKRTMTLVATDVAHQ",
    "response#3": "MNTSSSFKALASPPLISTSRPTTKSFPNPRFTSRFSPKPITCMRDSLNLGSNPKAPSPFSLATVSVDAPLGTKTSDKLRILVSEFRSLTEPIDRVKRLLNYAATLAPLDESARISENRVTGCTTQVWLEIKMDEFGRMRFKADSDSEISKGFCSCLIWILDGAKPEEVMGVRSEDLSEMNVGVHGKEQSRVNTWHNVLMSMQKRTMTLVATDVAHR"
  },
  {
    "reference": "MSILTSKHILLAVTGSIAAYKAADLASKLTQAGALVDVILTPAAEKFVTPLTFQSVTGRKAFTDADLWGGEAHVLHVGLGQGADLLVVAPATANTLAKMAHGLADNLATITALAARCPILVAPAMDVGMFEHPATQENVRILCGRGVYFAGPDEGRMASGLVGRGRFVEPAELLGHIRLTLGFGGKLAGKKIVVTAGGTQEPLDPVRVLTNRSSGKQGYALAQAALDAGGTVVLVAAPTALTPPVGARVVAVRTAEEMKEAVLAESAGAEALVMAAAVADFRPKQVAGNKLKKRDGIPQIELEAAPDVLLAVASTRLGAGAGMRSRKPRIVIGFAAESRDLIGNAESKLQSKNLDMIVANDISAPDAGFGVDTNRVTLLFADGRKISLPLMGKDEAAEAVIEHLAALLETQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should take into account the physiological conditions of the CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis pathway.2. The protein should be able to bind CTP ligand in a variety of conditions3. The protein have enhanced H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..5. The designed protein must possess phosphopantothenoylcysteine decarboxylase activity, FMN binding, metal ion binding to support pantothenate catabolic process under certain conditions.6. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "MRIVLGVGGGIAAYKVASLLRLFTEAGHEVTVIPTDAAQRFVGTATWEALSGNRVSNDVFDAVDQVRHVRLGQEADLVVVAPATADLLARAAAGMADDLLTGTLLVTRAPVVYAPAMHTEMWQHPATAANVATLRARGAVVLEPAVGRLTGADSGPGRLPEPDDIFAIAETVLADRGRLAGKSVVISAGGTREALDPVRYLGNRSSGKQGVALAKAAASQGAHVTLLAGAMDIQAPTHHNITVVRIESTQELLDAVMQHAPHKDALIMAAAVADFRPASVTNEKIKKEDDAAAPTIVLEQTEDVLRTSVERRATGQPLPRVIIGFAAETGDEEISPLEYGRAKLQRKGCEILVVNEVGVGKVFGQDDNDVTIVGADGVEQLAGRTVTITAGGTREPLDPVRFLGNRSSGKQGVALAAAALEAGATVQFIAAHLEVQPPPGAQLTVVETAEELREVTLKLAQRSDALVMAAAVADFRPDTVSDTKVKKRDDGENPVITLIRNPDILVEVVQQRAELGSPAVIVGFAAETGDDDATPLAHGQQKLQRKGCDLLILNRVGRSLVFGQDSTEVAILTRDGETFDVALGSKSDVADAVWDLVATVLAP",
    "response#2": "MRIVLGVGGGIAAYKVASLLRLFTEAGHEVTVIPTDAAQRFVGTATWEALSGNRVSNDVFDAVDQVRHVRLGQEADLVVVAPATADLLARAAAGMADDLLTGTLLVTRAPVVYAPAMHTEMWQHPATAANVATLRARGAVVLEPAVGRLTGADSGPGRLPEPDDIFAQAEAVLARGGALSGRHVVVSAGGTREPIDPVRFIGNRSSGKQGYALAAVAARRGARVTLVSANAALAVPDGVDLVEVETTVQLQEAVTAAGKDADAVVMAAAPADYRSAAYTDHKIKKTGSGDDLVLQLVENPDIVKGLVAARGAATAPVIVSFAAETGDATGSVMDHARAKFARKGSDLQVVNEVGPGVTFGQDEAVIHIVDRAGAVTLAGRTVTITAGGTREPLDPVRFLGNRSSGKQGVALAAAALEAGATVQFIAAHLEVQPPPGAQLTVVETAEELREVTLKLAQRSDALVMAAAVADFRPDTVSDTKVKKRDDGENPVITLIRNPDILVEVVQQRAELGSPAVIVGFAAETGDDDATPLAHGQQKLQRKGCDLLILNRVGRSLVFGQDSTEVAILGADGSETEVARGSKSEVADAVWDAVVALLA",
    "response#3": "MRIVLGVAGGIAAYKAVLLLRALREAGHEVRVVPTRAALEFVGRPTWEALSGQRVTTDVFEDVDEVAHVAIGQHADLVVVAPATADLLARAASGQADDLLTATLLVTRAPVLLAPAMHTEMWEHPATVANVATLRSRGVHVLDPASGRLTGADTGPGRLPEPDEIAAARVLRLLAAGGDLAGRRVVVSAGGTREPLDPVRYLGNRSSGRQGWAIALVAAARGADVTLVAANVDLVEPPGVRVRQVGTALELHEAMLETTVGADGGPVDAVVMTAAVADFRPMDPSAVKIKKAAGQGPPTIGLVENPDILAALSRPGPDRAAVVVGFAAETGDGTADPTTSVLDFGRDKLRRKGCDVLVVNRVDAGRAFGRPDNAAWLLTPGAEPRILLVIGGGIAAYKSLELIRRLRERGATVRAVMTPAACQFITPLSVGALTADRVFTDLFDREDEHDVGHIRLAREADLIVAAPATADLLAKMANGLANDLASTILLAAKVPVLVAPAMNPAMWQHPATRRNVAQLAGDGVAFVGPNSGAMAEAGEAGEGRMAEPLEIVAAIERALGLRRPVRLAGRRVVVSAGGTREPLDPVRYLGNRSSGRQGWAIALVAAARGADVTLVAANVDLVEPPGVRVRQVGTALELHEAMLETTVGADGGPVDAVVMTAAVADFRPMDPSAVKIKKAAGQGPPTIGLVENPDILAALSRPGPDRAAVVVGFAAETGDGTADPTTSVLDFGRDKLRRKGCDVLVVNRVDAGRAFGRPDNAAWLLAADGTEHLAGRRVVVSAGGTREPLDPVRYLGNRSSGRQGWAIALVAAARGADVTLVAANVDLVEPPGVRVRQVGTALELHEAMLETTVGADGGPVDAVVMTAAVADFRPMDPSAVKIKKAAGQGPPTIGLVENPDILAALSRPGPDRAAVVVGFAAETGDGTADPTTSVLDFGRDKLRRKGCDVLVVNRVDAGRAFGRPDNAAWL"
  },
  {
    "reference": "MRVRGIRRNYQPWWIWGILGFWMVMICNGGNNLWVTVYYGVPVWKEANATLFCASDAKAYEKEVHNVWATHACVPTDPNPQEMLLKNVTENFNMWENGMVDQMQEDVISLWDQSLKPCVKLTPLCVTLECKNISSNSSSNSNNSASDQELYNEIKNCSFNATTEIRDRNQAVDALFYKLDIVPLENNNGSNGNRSNTYRLINCNTSVITQACPKVSFDPIPIHYCAPAGYAILKCNNKTFNGTGPCSNVSTVQCTHGIKPVVSTQLLLNGSLAEKEIIIRSKNLTDNTKIIIVHLNESVEINCTRPNNNTRKSIRIGPGQTFYATGDIIGDIRQAHCNISRKKWEETLQRVSKKLAEHFLNKTIKFASSSGGDPEITTHSFNCRGEFFYCNTSGLFNGTYMSNGTYNETNNNSSSNITIPCRIKQVINLWQGVGRAMYAPPIAGKITCNSNITGLLLVRDGGTGNQTNDIETFRPQGGDMRDNWRSELYKYKVVEIKPLGIAPTEAKRRVVGREKRAVGLGAVLFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLMAIDAQQHLLQLTVWGIKQLQTRVLAIERYLKEQQLLGIWGCSGKLICTTAVPWNSSWSNKNLTDIWDNMTWMQWDREISNYTDTIYRLLEESQNQQEQNEKDLLALDSWKNLWNWFDITNWLWYIKIFIMVVGGLIGLRIVFAVISIVNRVRQGYSPLSFQTLTPTSVGPDRLERIEEGGGEQDRDRSIRLVSGFLALAWDDLRSL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should contain at least one YXXL motif; contains endocytosis signal motif to achieve its intended function.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The designed protein should contain one or more Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains that are essential for its function.4. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.5. Ensure the presence of a signal peptide within the protein.6. The protein must exhibit the following characteristics: Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.7. A protein that can perform structural molecule activity in viral envelope, virion membrane, plasma membrane, host cell endosome membrane to support clathrin-dependent endocytosis of virus by host cell, actin filament reorganization, fusion of virus membrane with host plasma membrane. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWKEANTTLFCASDAKAYDTEAHNVWATHACVPTDPNPQEVVLANVTEYFNMWKSNMVEQMHEDIISLWDQSLKPCVKLTPFCVTLNCSNATYTNSSENAKATPTPKTIVDPEMKNCSFNITTSMRDKMTKEQALFYALDVVPMDNDNDTIRDNGTASYRLRSCNTSVVTQACPKITFEPIPIHYCAPAGFALLKCNNKTFSGKGPCTNVSTVQCTHGIKPVVSTQLLLSGSLAEGEVILRSDNFTNNAKTIIVQLSTSVMIKCSRPSNNTSKSIHMGPGRAFYATERVIGNIRQAHCNLTRADWNRTLEKVVKKLGEQFENKTRISFNQPAGGDPEIVMHSFNCEGEFFYCNTTQLFNSTWVRDDANSSWRLNSSEGNDTLIILQCRIKQIINMWQEVGRAMYAPPIRGNISCLSNITGLILTRDGGIQNESQSQNGSESEETFRPVGGDMRDNWRSELYKYKVVKIEPLGVAPTRAKRRVVAREKRAVLGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLKAIEAQQQMLRLTVWGIKQLQARVLALERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNKTYKDIWDNMTWLQWDKEISNYTNEIYELLEKSQNQQEKNEQELLALDKWASLWNWFDITQWLWYIKIFIMIVGGLIGLRIVFAVLNIINRVRQGYSPLSFQTLTHHQREPDRPERIEEEGGEQDRGRSVRLVSG",
    "response#2": "MRVMGIQKNYPPLWRWGMIIFWIMIICNAEKLWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQEMILENVTENFNMWRNDMVEQMHEDVISLWDQSLKPCVKLTPLCVTLNCTDVKNTTLWVTVYYGVPVWKEATTTLFCASHAKSYETEVHNVWATHACVPTDPNPQEMVLENVTENFNMWKNEMVNQMHEDVISLWEQSLKPCVKLTPLCVTLECKNVECRNVSHNETGSLSNGTSRGITNCSFRNETYNEMKNCSFNATTEINDRKKKEHALFYRLDIVPLDNEKSNSSSNSSSEYYRLINCNTSTITQACPKVSFDPIPIHYCTPAGYAILKCNNKTFNGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIIRSENLTNNAKTIIVHLNESVEIECTRPNNNTRKSVRIGPGQTFYAPGEIIGDIRQAYCTIREDKWNNTLQRVGKKLAEHFPNKTIKFNSSSGGDLEITTHSFNCRGEFFYCNTSKLFNGTYMPNVTESTPSSNITNITIPCRIKQIINMWQEVGRAMYAPPIAGKITCKSNITGLLLVRDGGLEPNDTETFRPEGGDMRDNWRSELYKYKVVEIKPLGVAPTKAKRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKSQKEIWENMTWMQWDREISNYTNTIYSLLEESQIQQERNEKDLLALDSWKNLWSWFDISNWLWYIRIFIMIVGGLIGLRIIFTVLSIINRVRQGYSPLSLQT",
    "response#3": "MRVRGIQRNCQHLLTWGTMILGMIIICNAAENLWVTVYYGVPVWKEATTTLFCASHAKSYETEVHNVWATHACVPTDPNPQEMVLENVTENFNMWKNEMVNQMHEDVISLWEQSLKPCVKLTPLCVTLECKNVECRNVSHNETGSLSNGTSRGITNCSFRNETYNEMKNCSFNATTEINDRKKKEHALFYRLDIVPLDNEKSNSSSNSSSEYYRLINCNTSTITQACPKVSFDPIPIHYCTPAGYAILKCNNKTFNGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIIRSENLTNNAKTIIVHLNESVEIECTRPNNNTRKSVRIGPGQTFYAPGEIIGDIRQAYCTIREDKWNNTLQRVGKKLAEHFPNKTIKFNSSSGGDLEITTHSFNCRGEFFYCNTSKLFNGTYMPNVTESTPSSNITNITIPCRIKQIINMWQEVGRAMYAPPIAGKITCKSNITGLLLVRDGGLEPNDTETFRPEGGDMRDNWRSELYKYKVVEIKPLGVAPTKAKRRVVEREKRAVGLGAAFLGFLGAAGSTMGAASITLTVQARQVLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKTQTEIWDNMTWMQWDKEISNYTDTIYRLLEVSQNQQEQNEKDLLALDSWKNLWSWFSITKWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQT"
  },
  {
    "reference": "MSCPYAGNGNDHDDSAVPLTTEVGKIYGEYLMLDKLLDAQCMLSEEDKRPVHDEHLFIITHQAYELWFKQIIFEFDSIRDMLDAEVIDETKTLEIVKRLNRVVLILKLLVDQVPILETMTPLDFMDFRKYLAPASGFQSLQFRLIENKLGVLTEQRVRYNQKYSDVFSDEEARNSIRNSEKDPSLLELVQRWLERTPGLEESGFNFWAKFQESVDRFLEAQVQSAMEEPVEKAKNYRLMDIEKRREVYRSIFDPAVHDALVRRGDRRFSHRALQGAIMITFYRDEPRFSQPHQLLTLLMDIDSLITKWRYNHVIMVQRMIGSQQLGTGGSSGYQYLRSTLSDRYKVFLDLFNLSTFLIPREAIPPLDETIRKKLINKSV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The substrate, heme binding site should be stable and able to withstand changes in environmental conditions.2. The protein must exhibit the following characteristics: Catalyzes the oxidative cleavage of the indole moiety; Required during larval growth to control the level of potentially harmful free tryptophan in the hemolymph.3. The design objective is to achieve a protein with helix-helix-helix-helix-strand-helix-helix-helix-helix-helix-helix-helix-helix-helix-turn characteristics.4. The desired protein should be able to perform L-tryptophan + O2 = N-formyl-L-kynurenine5. The designed protein should have metal ion binding to facilitate tryptophan catabolic process, protein homotetramerization.6. Ideal protein should have a binding affinity for key enzymes in the Pigment biosynthesis, ommochrome biosynthesis pathway to enhance the overall efficiency of the pathway.7. The presence of heme is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "DLLTDSGTNAMSDHQWAAMITGDEAYAGSRNYYDLKDKAKELFNYDYIIPAHQGRGAENILFPVLLKYKQKEGKAKNPVFISNFHFDTTAAHVELNGCKAINIVTEKAFDSETYDDWKGDFDIKKLKENIAQHGADNIVAIVSTVTCNSAGGQPVSMSNLKEVYEIAKQHGIFVVMDSARFCENAYFIKARDPKYKNATIKEVIFDMYKYADALTMSAKKDPLLNIGGLVAIRDNEEIFTLARQRCVPMEGFVTYGGLAGRDMAAMVQGLEEGTEEEYLHYRIGQVKYLGDRLREAGIPIQYPTGGHAVFVDCKKLVPQIPGDQFPAQAVINALYLESGVRAVEIGSFLLGRDPATGEQKHADMEFMRLTIARRVYTNDHMDYIADGWDELLDLLTDSGTNAMSDHQWAAMITGDEAYAGSRNYYDLKDKAKELFNYDYIIPAHQGRGAENILFPVLLKYKQKEGKAKNPVFISNFHFDTTAAHVELNGCKAINIVTEKAFDSETYDDWKGDFDIKKLKENIAQHGADNIVAIVSTVTCNSAGGQPVSMSNLKEVYEIAKQHGIFVVMDSARFCENAYFIKARDPKYKNATIKEVIFDMYKYADALTMSAKKDPLLNIGGLVAIRDNEEIFTLARQRCVPMEGFVTYGGLAGRDMAAMVQGLEEGTEEEYLHYRIGQVKYLGDRLREAGIPIQYPTGGHAVFVDCKKLVPQIPGDQFPAQAVINALYLESGVRAVEIGSFLLGRDPATGEQKHADMEFMRLTIARRVYTNDHMDYIA",
    "response#2": "GRDSRLSSRSNSALTLTDTEQDNKSDQENDQRTSQQGPPPLPPAPYQHKQHPSVTSLNQSLANKAAELQSASECVQLQENWMLGGNVGLENRHFLLKTGTGTTSLFTTPNPGYTMATGAVYSPPARPLTRNPLSRGAFKLNKSSKRCSWKLV",
    "response#3": "GRDSRLSSRSNSALTLTDTEQDNKSDQENDQRTSQQGPPPLPPAPYQHKQHPSVTSLNQSLANKAAELQSASECVQLQENWMLGGNVGLENRHFLLKTGTGTTSLFTTPNPGYTMATGAVYSPPARPLTRNPLSRGAFKLNKSSKRCSWKDDLLTDSGTNAMSDHQWAAMITGDEAYAGSRNYYDLKDKAKELFNYDYIIPAHQGRGAENILFPVLLKYKQKEGKAKNPVFISNFHFDTTAAHVELNGCKAINIVTEKAFDSETYDDWKGDFDIKKLKENIAQHGADNIVAIVSTVTCNSAGGQPVSMSNLKEVYEIAKQHGIFVVMDSARFCENAYFIKARDPKYKNATIKEVIFDMYKYADALTMSAKKDPLLNIGGLVAIRDNEEIFTLARQRCVPMEGFVTYGGLAGRDMAAMVQGLEEGTEEEYLHYRIGQVKYLGDRLREAGIPIQYPTGGHAVFVDCKKLVPQIPGDQFPAQAVINALYLESGVRAVEIGSFLLGRDPATGEQKHADMEFMRLTIARRVYTNDHMDYIARENLPKGFDTEDLLTDSGTNAMSDHQWAAMITGDEAYAGSRNYYDLKDKAKELFNYDYIIPAHQGRGAENILFPVLLKYKQKEGKAKNPVFISNFHFDTTAAHVELNGCKAINIVTEKAFDSETYDDWKGDFDIKKLKENIAQHGADNIVAIVSTVTCNSAGGQPVSMSNLKEVYEIAKQHGIFVVMDSARFCENAYFIKARDPKYKNATIKEVIFDMYKYADALTMSAKKDPLLNIGGLVAIRDNEEIFTLARQRCVPMEGFVTYGGLAGRDMAAMVQGLEEGTEEEYLHYRIGQVKYLGDRLREAGIPIQYPTGGHAVFVDCKKLVPQIPGDQFPAQAVINALYLESGVRAVEIGSFLLGRDPATGEQKHADMEFMRLTIARRVYTNDHMDYIA"
  },
  {
    "reference": "MLLEMDKIKITVDSKIGNVVTISYNLEKITIDVTPKKKKEKDVLLAQSVAVEEAKDVKVEEKNIIDIEDDDDMDVESA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Specifically, inhibits the initiation of cap-dependent and cap- independent translation. The designed protein sequence is ",
    "response#1": "MRNNSSDVDAKLTSFMEEAQNSTNSEEMLGEIVRTIYQKAVSDRSFAFTAAKLCDKMALFMVEGTKFRSLLLNMLQKDFTVREELQQQDVERWLGFITFLCEVFGTMRSSTGEPFRVLVCPIYTCLRELLQSQDVKEDAVLCCSMELQSTGRLLEEQLPEMMTELLASARDKMLCPSESMLTRSLLLEVIELHAI",
    "response#2": "MRNNSSDVDAKLTSFMEEAQNSTNSEEMLGEIVRTIYQKAVSDRSFAFTAAKLCDKMALFMVEGTKFRSLLLNMLQKDFTVREELQQQDVERWLGFITFLCEVFGTMRSSTGEPFRVLVCPIYTCLRELLQSQDVKEDAVLCCSMELQSTGRLLEEQLPEMMTELLASARDKMLCPSESMLTRSLLLEVIELHAK",
    "response#3": "MRNNSSDVDAKLTSFMEEAQNSTNSEEMLGEIVRTIYQKAVSDRSFAFTAAKLCDKMALFMVEGTKFRSLLLNMLQKDFTVREELQQQDVERWLGFITFLCEVFGTMRSSTGEPFRVLVCPIYTCLRELLQSQDVKEDAVLCCSMELQSTGRLLEEQLPEMMTELLASARDKMLCPSESMLTRSLLLEVIELHAQ"
  },
  {
    "reference": "MEQSRSQQRGGEQSWWGSDPQYQYMPFEHCTSYGLPSENGGLQHRLRKDAGPRHNVHPTQIYGHHKEQFSDREQDIGMPKKTGSSSTVDSKDEDHYSKCQDCIHRLGQVVRRKLGEDGIFLVLLGLLMALVSWSMDYVSAKSLQAYKWSYAQMQPSLPLQFLVWVTFPLVLILFSALFCHLISPQAVGSGIPEMKTILRGVVLKEYLTMKAFVAKVVALTAGLGSGIPVGKEGPFVHIASICAAVLSKFMSVFCGVYEQPYYYSDILTVGCAVGVGCCFGTPLGGVLFSIEVTSTYFAVRNYWRGFFAATFSAFVFRVLAVWNKDAVTITALFRTNFRMDFPFDLKELPAFAAIGICCGLLGAVFVYLHRQVMLGVRKHKALSQFLAKHRLLYPGIVTFVIASFTFPPGMGQFMAGELMPREAISTLFDNNTWVKHAGDPESLGQSAVWIHPRVNVVIIIFLFFVMKFWMSIVATTMPIPCGGFMPVFVLGAAFGRLVGEIMAMLFPDGILFDDIIYKILPGGYAVIGAAALTGAVSHTVSTAVICFELTGQIAHILPMMVAVILANMVAQSLQPSLYDSIIQVKKLPYLPDLGWNQLSKYTIFVEDIMVRDVKFVSASYTYGELRTLLQTTTVKTLPLVDSKDSMILLGSVERSELQALLQRHLCPERRLRAAQEMARKLSELPYDGKARLAGEGLPGAPPGRPESFAFVDEDEDEDLSGKSELPPSLALHPSTTAPLSPEEPNGPLPGHKQQPEAPEPAGQRPSIF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein have CBS domains with specific sequence motifs that are crucial for its activity.2. The protein have enhanced 2 chloride(in) + H(+)(out) = 2 chloride(out) + H(+)(in).3. The Extracellular topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.4. The designed protein should be involved in chloride transmembrane transport, regulation of ion transmembrane transport, muscle contraction by localizing to plasma membrane, chloride channel complex and exhibiting protein homodimerization activity, voltage-gated chloride channel activity.5. The protein must exhibit the following characteristics: The absence of conserved gating glutamate residues is typical for family members that function as channels (Probable).; The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (Probable).6. The designed protein should contain at least one Selectivity filter part_1, Selectivity filter part_3 motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "MMIMGELEPSDGKIKHSGRISFSPQVSWIMPGTIKENIIFGVSYDEYRYKSVVKACQLEEEISKFPEKDYTVLGDGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDILTEKEIFESCVCKLMANKTRILVTSKLEHLRTADKILILHEGTCYFYGTFSQLQDQRPDFSSNLMKLTVLHPPPALKDINFKIERGQLLAVAGSTGAGKTSLLMMIMGELEPSEGKIKHSGRISFCSQFSWIMPGTIKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPLFPVTCLRRREKVGVIVDVLSDTASNHNGFPVVEHADDTQPARLQGLILRSQLIVLLPPYKNLEEPEGVKILRFSSPIFYGNVDGFKKCINSTVGFDAIRVYNKRLKALRRIQKLIKKGQLRATKNGIISDIGSSNNAFEPDEDVEEPEELNIPTKEIEIQVDWNSELPVKVNVPKVPIHSLVLDCGAVSFLDVVGVRSLRMIVKEFQRIDVNVYFALLQDDVLEKMEQCGFFDDNIRKDRFFLTVHDLQEGNGCNAQDIQGIKIITYCSPLYFANSEIFRQKVIAKTGMDPQKVLLAKQKYLKKQEKRRMRPTQQRRSLFMKTKTVSLQELQQDFENAPPTDPNNNQTPANGTSVSYITFSPDSSSPAQSEPPASAEAPGEPSDMLASVPPFVTFHTLILDMSGVSFVDLMGIKALAKLSSTYGKIGVKVFLVNIHAQVYNDISHGGVFEDGSLECKHVFPSIHD",
    "response#2": "MIMGELEPSDGKIKHSGRISFSPQVSWIMPGTIKENIIFGVSYDEYRYKSVVKACQLEEEISKFPEKDYTVLGDGGITLSGGQRARISLARAVYKDADLYLLDSPGQQVTTGLELDKITAFWDEGSGEIFTKTKQEHGNNKIPSTNDGLFFSNFTLHFTPVLKDINFKIEKGKLLAVAGSTGAGKTSLLMLIMGELEPLEGKIKHSGRISFCPQVSWIMPGTIKENIIFGVSYDEYRYKSVIKACQLEEDISKFPEKDDTVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDMLTEKEIFKSCVCKLIVNKTRILVTSKLEHLKIADKILILHGGCCYFYGTFSELQGQRPNFSSELMGWDAFDHYSPERRNSILTETLRRLVQPQISGRIIASFDPFHAPERSQGYYLALGLCLLFVARFLLLQPALFGLHHLGMQIRIALFSLIYKKTLKLSSRVLDKISTGQLVSLMSAHLNKLDESLGLAHFVWITPLQCILCTGLIWELIEVNGFCALASLTLLGIIQAWLSQKMGPHRAKRAGMISRRLALTTEIVENIHSVKAYGWEEVMETIIKNIRQDEMTLTRKIGSLRYFYSASYFFSAIVVIISAIFFGVLLYLGEASKTVQPQISGRIIASFDPFHAPERSQGYYLALGLCLLFVARFLLLQPALFGLHHLGMQIRIALFSLIYKKTLKLSSRVLDKISTGQLVSLMSAHLNKLDESLGLAHFVWITPLQCILCTGLIWELIEVNGFCALASLTLLGIIQAWLSQKMGPHRAKRAGMISRRLALTTEIVENIHSVKAYGWEEVMETIIKNIRQDEMTLTRKIGSLRYFYSASYFFSAIVVIISAIVPHALSKGIILRRIFTTASYCIVLRM",
    "response#3": "MIMGELEPSDGKIKHSGRISFSPQVSWIMPGTIKENIIFGVSYDEYRYKSVVKACQLEEEISKFPEKDYTVLGDGGITLSGGQRARISLARAVYKDADLYLLDSPHPNGDGLFFTNLYVTPVLKNISLHLHKGEMLAVAGSTGSGKSSLLMTILGELVPSSGRIRHSGRVSFSSQTAWIMPGTIRDNIVFGLTYDEIRYTSVVKSCQLEEDLAALPEKDRTPLAEGGLNLSGGQKARVALARAVYRDADTYLLDAPFTHLDIATEKEIFDKCLCRLMASKTRIVVTNKIEHLKRADKILLLHNGESFFYGTFSELQSQRPDFSSLLLLRNISLYLEKGQMLAVAGSTGSGKSSLLMMILGELVPSEGKIRHSGRISFSPQTSWIMPGTIRDNILFGLTYDEYRYTSVIKACQLEEDFALLPEKDKTPLIEGGVTLSGGQRARLCLARAVYKDADLYLLDAPHDLGSDRSTNDGLFFSNFTLHFTPVLKDINFKIEKGKLLAVAGSTGAGKTSLLMLIMGELEPLEGKIKHSGRISFCPQVSWIMPGTIKENIIFGVSYDEYRYKSVIKACQLEEDISKFPEKDDTVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDMLTEKEIFKSCVCKLIVNKTRILVTSKLEHLKIADKILILHGGCCYFYGTFSELQGQRPNFSSELMLHTLSVHPRCPEIRNYFGEKIGIYFVFLGYYTEMLLFAALVGLACFIYGLLSMENNRTSTEICDPDIGGQMIMCPLCDEVCDYWRLNTTCLHSKFSHLFDNESTVFFALFMGIWVTLFLEFWKQRQARLEYEWDLVDFEEEQQQLQLRPEFEAMCKHKKMNPVTKEMEPHMPLCHRIPWYFVSGTTVTFGMALLLSSMVSILIYRLSVFATFASFMESEATLQSVKSFFTPQLATALSGSCLNCIVILILNFFYEKISAWITKMEIPRTHQEYESSLTLKMFLFQFVNYYSSCFYVAFFKGKFVGYPGSYTYMFNIWRSEECGPAGCLIELTTQLTIIMIGKQIFGNIHEAFQPLIFNWWRRRRARTHSEKLYSRWEQDHDLQVYGHRGLFYEYLETVIQFGFATLFVASFPLAPLFALMNNIMGIRVDAWKLTTQYRRPVAAKAHSIGVWQDILFGMAIVSVATNAFIVSFTSD"
  },
  {
    "reference": "MSLRKRSAQTQMWESPVMSQGKQSLLELPLKESPPKDSGLKAVPSTHTLYVSHLNPQFSVPVLACLLRDTLERLELPVARGQIEVVRRPRNTYALVQVAAPKAVLASLPWRLQMALEEQLILKELTARGKELVLSEALESLHHREQEDSGPSPSHSPGPSPGPSPGLRHPPLPQLADPSPIWGSAGRRQISQNRPSGVRSDSAIVHHKILGQEQLFQGAFLGSETRNMEFKRGSGEYLSLAFKHHVRRYVCAFLNSEGGSLLVGVEDSGLVQGIHCSHRDEDRTRLLVDSILQGFKPQVFPDAYTLTFIPVISTTTTSTPLKVLRLTVHTPKAQGEPQLYETDQGEVFLRRDGSIQGPLSVGAIQDWCRQKWAMELGKLEEKVKVLTLEKEQLQQQLRQRQPLSCSCCVL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "YTTLEIPGARSFGGSGGYGEVQLIREHKLAVKTIREKEWFAVELVATLLVGECALRGGRTHDIRGFITPLGFSLQQRQIVFPAYDMDLGKYIGQLASLRATTPSVATALHHCFTDLARAVVFLNTRCGISHLDIKCANVLVMLRSDAVSLRPAVLADFSLVTLNSNSTISRGQFCLQEPDLESPRGFGMPAALTTANFHTLVGHGYNQPPELSVKYLNNERAEFNNRPLKHDVGLAVDLYALGQTLLELLVSVYVAPSLGVPVTRVPGYQYFNNQLSPDFAVALLAYRCVLHPALFVNSAETNTHGLAYDVPEGIRRHLRNPKIRRAFTEQCINYQRTHKAVLSSVSLPPELRPLLVLVSRLCHANPAARHSLC",
    "response#2": "PGARSFGGSGGYGEVQLIREHKLAVKTIREKEWFAVELVATLLVGECALRGGRTHDIRGFITPLGFSLQQRQIVFPAYDMDLGKYIGQLASLRATTPSVATALHHCFTDLARAVVFLNTRCGISHLDIKCANVLVMLRSDAVSLRPAVLADFSLVTLNSNSTISRGQFCLQEPDLESPRGFGMPAALTTANFHTLVGHGYNQPPELSVKYLNNERAEFNNRPLKHDVGLAVDLYALGQTLLELLVSVYVAPSLGVPVTRVPGYQYFNNQLSPDFAVALLAYRCVLHPALFVNSAETNTHGLAYDVPEGIRRHLRNPKIRRAFTEQCINYQRTHKAVLSSVSLPPELRPLLVLVSRLCHANPAARHSLQ",
    "response#3": "YTTLEIPGARSFGGSGGYGEVQLIREHKLAVKTIREKEWFAVELVATLLVGECALRGGRTHDIRGFITPLGFSLQQRQIVFPAYDMDLGKYIGQLASLRATTPSVATALHHCFTDLARAVVFLNTRCGISHLDIKCANVLVMLRSDAVSLRPAVLADFSLVTLNSNSTISRGQFCLQEPDLESPRGFGMPAALTTANFHTLVGHGYNQPPELSVKYLNNERAEFNNRPLKHDVGLAVDLYALGQTLLELLVSVYVAPSLGVPVTRVPGYQYFNNQLSPDFAVALLAYRCVLHPALFVNSAETNTHGLAYDVPEGIRRHLRNPKIRRAFTEQCINYQRTHKAVLSSVSLPPELRPLLVLVSRLCHANPAARHSLC"
  },
  {
    "reference": "MAQLKVTQTRSTIGGKQNQRETLATLGLGRIGKSTVQEDTPGVRGMIRVVAHLVSVEEVDES",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a specific function in translation and should be localized to cytoplasm in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "SMELLFLDTFKHPSAEQSSHIDVVRFPCVVYINEVRVIPPGVRAHSGLPDNRAYGETSPHTFQLDLFFNNVSKPSAPVFDRLGSLEYDENTSIIFRPNSKVNTDGLVLRGWYNCLTLAIYGSVDRVISHDRDSPPPPPPPPPPPQPQPTLKRNLKHADGEKEDQFNGSPPRPQPRGPRTPPGPPPPDDDEDDPMSLPVSGDKEEDVPHREDYFEPISPDRNSVPQEGQYSDEGEVEEEPQEEGEDDEDDVDVEEEEDEDEC",
    "response#2": "SMELLFLDTFKHPSAEQSSHIDVVRFPCVVYINEVRVIPPGVRAHSGLPDNRAYGETSPHTFQLDLFFNNVSKPSAPVFDRLGSLEYDENTSIIFRPNSKVNTDGLVLRGWYNCLTLAIYGSVDRVISHDRDSPPPPPPPPPPPQPQPTLKRNLKHADGEKEDQFNGSPPRPQPRGPRTPPGPPPPDDDEDDPMSLPVSGDKEEDVPHREDYFEPISPDRNSVPQEGQYSDEGEVEEEPQEEGEDDEDDVDVEEEEDEDEC",
    "response#3": "SMELLFLDTFKHPSAEQSSHIDVVRFPCVVYINEVRVIPPGVRAHSGLPDNRAYGETSPHTFQLDLFFNNVSKPSAPVFDRLGSLEYDENTSIIFRPNSKVNTDGLVLRGWYNCLTLAIYGSVDRVISHDRDSPPPPPPPPPPPQPQPTLKRNLKHADGEKEDQFNGSPPRPQPRGPRTPPGPPPPDDDEDDPMSLPVSGDKEEDVPHREDYFEPISPDRNSVPQEGQYSDEGEVEEEPQEEGEDDEDDVDVEEEEDEDEC"
  },
  {
    "reference": "MSTPSIWSIVLAAGNGSRLAKAGINERKQFIDYQGVPLFWHSLRTFSRIALVRGIMLVVPETELDRVTAWVNQLLDAEPLGMPIVCVKGGARRQDSVYQALCHLPEDCNRVFVHDAARPFFTPQCVHKLLEAVTSDVVGAVPGCAVTDTVKQVHGARVLRTLDRSCLMAVQTPQLFDRNTLQQAHAKALQDNWNVTDDASMVEMMNKNVVVVDGDPGNVKITLPEDLRHLALQQETPVPVSGLGYDVHAYGGNRPMVIGGVPIPGGPDIKAHSDGDVLIHALCDAILGCIGQGDIGDLFPDSDATFENMPSSAFLAEVLDRAMQHGLTMTHVDMTVVAQTPRLSPFKVQIKKNLACLMALPFDRVNLKATTEEGLGFTGTKQGIKAMALVTGTINKPRPVP",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design must be able to perform metal ion binding in order to contribute to terpenoid biosynthetic process.2. The a divalent metal cation binding site should be located in a region of the protein that is accessible to the ligand.3. A protein should be able to manipulate the activity of enzymes in the Isoprenoid biosynthesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "MRVGTGFDVHSFVEGDHLWLCGLKIAHSHGLNGHSDSDVGMHALTDAIYGALAQGDIGQHFPPSDMQWKGAPSDIFLRHAVDLAQEMGYRISNCDVTLICERPKIGPHAQAMRARLAQIMGLEVARVSVKATTSEKLGFTGRGEGIAAQASALL",
    "response#2": "LPVDSEHAALKFLLEGKKNIAKLYITASGGAFYRYKIKDLNQVSVKDALKHPNWNMGAKITIDSATMANKLFEIIEAYHLR",
    "response#3": "IRTGNGFDVHAFEDGDHVMLCGVRVPHDRALKGHSDADVGMHAVTDAIYGALAEGDIGRHFPPSDPQWKAAASHIFLSHAVELAASRGYRITNVDCTMICERPKVGPHAPAMIEEMARILLIEPDRVSVKATTSERLGFTGREEGIAAIATATR"
  },
  {
    "reference": "MKFLLGNINSTVLTMAGLRVLSSMIELTAAIVMLVTNDVRKAVVVNSILAIVGPLIFIITMTVGIYQIAGQLSYAKLILIFTGVVLILAGVHK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein localizes to the plasma membrane.2. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "KGALLVCLAATMWGFDGIALTPRLFSLHVPFVVFILHLLPLILMSILFGKEEVKNIKKLQKNDLFFFFCVALFGGCLGTLCIVKALFLVNFKHLTVVTLLQKLQPIFAIILARLLLKEKLKRAYLFWGFLALLGGYLLF",
    "response#2": "KGALLVCLAATMWGFDGIALTPRLFSLHVPFVVFILHLLPLILMSILFGKEEVKNIKKLQKNDLFFFFCVALFGGCLGTLCIVKALFLVNFKHLTVVTLLQKLQPIFAIILARLLLKEKLKRAYLFWGFLALLGGYLLASLYSLLAAFSFGSATVFGKRILKSASFRTALYLRYLMTSCIMFVIVTFTSGFGDFLVATAGNWLIFVIIALTTGSGAILLYYFGLRYITAKVATMCELCFPISSVVFDYLINGNVLSPVQIASAILMIISIIKK",
    "response#3": "KGALLVCLAATMWGFDGIALTPRLFSLHVPFVVFILHLLPLILMSILFGKEEVKNIKKLQKNDLFFFFCVALFGGCLGTLCIVKALFLVNFKHLTVVTLLQKLQPIFAIILARLLLKEKLKRAYLFWGFLALLGGYLLASLYSLLAAFSFGSATVFGKRILKSASFRTALYLRYLMTSCIMFVIVTFTSGFGDFLVATAGNWLIFVIIALTTGSGAILLYYFGLRYITAKVATMCELCFPISSVVFDYLINGNVLSPVQIASAILMIISIIKQ"
  },
  {
    "reference": "MPLTGKRILIGLSGGIAAYKSAELVRRLKERGAEVRVVMTESAKSFITPLTLQAVSGEPVSDALLDPAAEAGMGHIELAKWADLVLIAPASANTLARLSAGLADDLLTTLCLATPAPVAVAPAMNQQMYRHPATQDNLATLARRQTLVWGPAQGAQACGDIGPGRMLEPMELVTLIERHFTPKTQLLSGIRLMLTAGPTREALDPVRFISNHSSGKMGFALAQAAVELGAEVTLVSGPVSLATPAGVRRLDVESAAQMHSVVMEHIDAQQIFIACAAVADYAPKTVASHKIKKTTADTMVIELTRNPDIVANVAALPNKPFTVGFAAETRDVEKYARDKLGRKGLDMIAANNVAKEGQGFNADTNALTVFWPTGQTDLPLAGKNELAGHLLTLIAEKYHDTH",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein have Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains with specific sequence motifs that are crucial for its activity.2. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.3. The protein must bind to Mg(2+) in order to perform its enzymatic function.4. The designed protein should be optimized for phosphopantothenate--cysteine ligase activity, metal ion binding activity in order to improve coenzyme A biosynthetic process, pantothenate catabolic process.5. We require a protein that can specifically bind to the metabolites produced in the CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis pathway.6. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A.7. The designed protein is the optimization of the Proton donor to achieve high catalytic efficiency for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2. The designed protein sequence is ",
    "response#1": "KRLVLGVTGGIAAYKVAELVRLLSKEGASVQVVMSEAATHFVTPVTFQALSGKPVFTHQWDPRVANNMAHIDLSRDADALVVAPASADFLAKLAHGLADDLLTTLVLARDCPLLVAPAMNRQMWENPATRRNIATLRGDGIEIVGPASGEQACGETGPGRMLEPEELLAAVVRALGDDAAAAGRAVAVSAGSTWESWDDIRFLANRSTGRMGRELALAAWLRGARVTLIAGPTVPEPPTLPNLIFIRVESTCDLLAALKGTDFDTLIMAAAPADFRPAHRVTGKVKKKVNPPALTLATNPDILKTLPRWVGEVVVGFAAEDDDLVNRARAKLADKNLDLVVANQAGGPGGAFGSPNNHIRLITAEGVEDWPRMSKDEVAGRLAARAAEALGEKG",
    "response#2": "KKVLLGITGGIAAYKSAELCRNLKKAGAEVRVVMTKGAQAFITPLTLQALSGNPVHHDLLDPAAEAAMGHIELARWAHFIVIAPASADFLARLNAGMADDLLTTLCLATNSSIAVAPAMNQQMWANPATQNNIQQLIKKGIEIWGPDEGDQACGDVGPGRMLEPADLLNRIDDPSGIAAACEQIIQGKRILISGGGTRENLDPVRFLGNPSTGKMGLALAQAASDRGAIVTLIHGPIAPELLPQSPNYNLIPIVTAEEMEAAMINHLKESEIIIMSAAVADVKPAHYTNYKLPKKELSLNLKLESVVDILAKLGTLKKDYQTLIGFAAQTGDIVNPALDKLKRKNLDIIVANPIDKAEAGFGTNTNQAIFLIDGAGEMTEIPTISKXNLAHXILDXASTKLXGKRILLIIGGGIAAYKSLELIRELARRGIASRCILTKAGAEFVTPLSVSALSGEKCFTDLFSLDDEAEMGHIQLSRSADLVVVCPATADLLAKAAHGHANDLASTTLMATDKPVLMVPAMNVRMWEHAATKRNVATLRADGTAVIEPDEGAMACGEFGPGRLPEVLAIADEVEAXGXXFXXXETXIAGRAVAVSAGSTWESWDDIRFLANRSTGRMGRELALAAWLRGARVTLIAGPTVPEPPTLPNLIFIRVESTCDLLAALKGTDFDTLIMAAAPADFRPAHRVTGKVKKKVNPPALTLATNPDILKTLPRWVGEVVVGFAAEDDDLVNRARAKLADKNLDLVVANQAGGPGGAFGSPNNHIRL",
    "response#3": "KTVLVCVTGCIAAYKSCEIVRLLQKQGIRVKVLMTEHATQFVGPTTFRALTHEKVAIDLFDDPTDPIHHISLAQEPDLVLVAPATANVIAKMAHGIADDLMSTTLLATEKPVLVAPAMNAGMWRAEATRENVETLRRRGVTIVGPGSGYLACGDVDTGRLADPEDIVAATLALAARLLDEGPAGRAVAVSAGSTWESWDDIRFLANRSTGRMGRELALAAWLRGARVTLIAGPTVPEPPTLPNLIFIRVESTCDLLAALKGTDFDTLIMAAAPADFRPAHRVTGKVKKKVNPPALTLATNPDILKTLPRWVGEVVVGFAAEDDDLVNRARAKLADKNLDLVVANQAGGPGGAFGSPNNHIRLLTADGSTDLPATSKEDLADAVWDAVVQRLPA"
  },
  {
    "reference": "MNAMLPITSLPTQLQLQSYMKPGAQMTGDTRVLKAGDVMLAYPMGNARQLSDNRPFIGHALSLGAALVLYEPQGLSQVVSAEDLGLIEQDSRCIAVENLTEQAGVLAAYWYGQPSQAMQVIGVTGTNGKTTITQWVAQALRAKQGKSAVMGTLGAGLLGATQKTGFTTPDAPRTHALLKDMLDQGATSVAIEVSSHALDQGRVNGVEFDVVVVSNLTQDHLDYHGDMQEYANAKKKILHLPHVKHIIINADDNFGQVCLAELAKNIEPETTVWAYATQAEKLLSLPCYAAGKMRKILAEDIHLAHQGMTFNWRLDGQVGGPIKTQMIGLFNVSNALAVMATLLANGCAATELTSLIEQLKPVMGRMELVSGKAQHSPMAIVDFAHTPDALEQTLKTLRVIAKERSGQLWCIFGCGGDRDPSKRPVMGGIAERLSDHVMVTSDNPRSEDPQKIISEVVNGCEAPEQVQTNSDRATAILQVIRQAKEQDVVLVAGKGHEDTQEIAGKRHAFSDQLHLQIAMGGAAV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity to facilitate regulation of cell shape, cell wall organization, cell cycle.2. The protein design should consider the endogenous proteins and enzymes present in the peptidoglycan biosynthesis pathway to avoid interference with native metabolic processes.3. The designed protein should contain one or more Mur ligase C-terminal domains that are essential for its function.4. The presence of Mg(2+) is necessary for the protein to function in the desired environment.5. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..6. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding. The designed protein sequence is ",
    "response#1": "MKLSEIISGIEVIESVGNVDTEIKDIEFDSRKIKKENLFIAQKGVVLDGHDFIFNAIEQGAVAVVCEKLPKKCATGIVYVIVASASEALGHIAANFYGNPSRELLLVGVTGTNGKTTVASILRGVLEYSKKSAGLIGTIRNKIGDKEIPAERTTPESLELQNIFFKMKDEGVTHCVMEVSSHALGLYRVDGCVFDVGVFTNLEPDHIGENEHASFEDYRNCKGLLFRQCRLGIINVDDRWFDIIVEGTSCKIETFGLGKDADYRASNLELISPGLLGVNYRVNGAFSIDVRLNIPGRFSVYNALAAIGACVMLDMSLDNIREGLEAVKVESGRMQRVDRGQDFLAVVDYAHKPAAVESVIATLREYIKGSGGRLAVVVGAGGDRDAGKRPLMGATAARGADLLIITDDNPRSEDPAVIRDAIRGGALGIAEAERGEVVEIGDRAAAITAAVDWARPGDVVLVAGKGHETYQILKDRTIHFDDREEAMKALKERGF",
    "response#2": "MRLSDLLKGVPLQGDEELVSGITHDSRQVRPGDLYAALPGAVTHGARFIDAAVGAGAAAVLTDEEGAEIAGWAAPDTPAGGSVSGAVPMIVVDHPRAWLGGIAAAVASNHYQNPAEKLQVVGVTGTNGKTTVASLLFELFTSIGYPCGLISTVKISYQNSEWDNSHTTPDAISLNKHLSKMTISGISHCFMELSSHGIAQRRANGLHFNGGVFTNLTHDHLDYHGDFKSYRDTKKLFFDQLPKTAFALTNIDDKNGTFMFQNCIASKHTYAVKQYADFKAQILEYEFSGMLLKIQGEEVWTNLVGEFNIQNLLAVYAVYGTAVLLGQDEAAVLRALSTLRPVAGRMQLVAQRENGATVFVDYAHKPGAVIAALGSLRPHVMGRIVCVIGAGGDRDRGKRSLMGQAARDFADVVIVTDDNPRTEDPAAIRAAVMEGAGPEATEIPDRAEAILRGVDALLPGDALLICGKGHEDYQILGTEKIHFDDREQAREALALRVKG",
    "response#3": "MKLDKLLKGIDYKKIKGDLDKEITSVAYDSRQVIPGSLFIALRGLKADGSQFIREAIAKGAAAIISESEISTGKDVPIVLVENARKALAELASRFYDNPSKKLKLVGVTGTNGKTTVASLLFELFTSIGYPCGLISTVKISYQNSEWDNSHTTPDAISLNKHLSKMTISGISHCFMELSSHGIAQRRANGLHFNGGVFTNLTHDHLDYHGDFKSYRDTKKLFFDQLPKTAFALTNIDDKNGTFMFQNCIASKHTYAVKQYADFKAQILEYEFSGMLLKIQGEEVWTNLVGEFNIQNLLAVYAVGTALTFGLDVDRIKEALESIEGVPGRMEQVRVDDPDAPMMVVDYAHTPDAIENVLRALRSQTPGRPAGRLVVVTGAGGNRDRDKRPLMGAAAARNADVVIVTDDNPRLEHPPEIRAAVLAGAERISEEERAEVVEVADRRAAIRAALARAHGPQDTVVVLGKGHEDYQIVKGEKRHFDEREVVREILG"
  },
  {
    "reference": "MSERSPVITVDGPSGAGKGTICQLLAQHLGWHLLDSGAIYRVLALAAIHHDVELENEEALTLLAAHLDVQFLTGTEADAIKVILEGEDVTTTIRTQECSNAASKVAALPRVREALLRRQRAFSAAPGLVADGRDMGTVVFSKAPVKIFLTASAEERAQRRYNQLQDKGFDVKIDRLLAEIIERDDRDTNRAASPLVPADDALVIDTSGIGIEDVLKQVLEYVGQKITVT",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have CMP kinase activity, ATP binding within cytoplasm to regulate pyrimidine nucleotide metabolic process, phosphorylation.2. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.3. The protein should have a fast turnover rate for ATP + dCMP = ADP + dCDP. The designed protein sequence is ",
    "response#1": "TIDGPSGVGKGTVSKIVAARLGWHYLDSGALYRAVAVAADWAAVDVSDTTALVKCAFDTCVNFAECADGEMRVLVNSIDATDVLRMETTGVLASTIASISEVRATLKKRQQMFRRTPGLVADGRDMGTVIFPDAKYKVFLTAKAEERAQRRYKQLMKKGVSVMFGALLEEIRARDARDACRSVAPLKPADDALLIDSTCMEVDEVVAQVLALRIAIDGPAGAGKSTVARILAKKLSFTYLDTGAMYRAVTVLFLENNLSLEDENGIKRLLEKTDIKIIPGDEGQKILLNERDVTELIRTPQVSELVAKVSALPEVRKYLTELQRKIIAKGNVVADGRDIGTVVMPEAEVKIFLTASAEERARRRHRELLAKGYEVSYEEVFREVLKRDELDTTREISPLRKAEDAILVDTSTLSVEQSVETILNWYQERR",
    "response#2": "VIAVSGQPGSGKTTIAREIARVLGLPLVSSGLLFREMAARMGMDFIEFHKYAETNPDIDKKVDSLAIERAKAGDVVLEGHLTAWIVRPYADVCIYLKASLETRARRVALRDGKSLQDALREVAEREELNRRRYLSIYGIDINDLSIFDLVLDTR",
    "response#3": "VIAVSGQPGSGKTTIAREIARVLGLPLVSSGLLFREMAARMGMDFIEFHKYAETNPDIDKKVDSLAIERAKAGDVVLEGHLTAWIVRPYADVCIYLKASLETRARRVALRDGKSLQDALREVAEREELNRRRYLSIYGIDINDLSIFDLVLDTR"
  },
  {
    "reference": "MADKKGTLEDVNEWVLVEAECSDVDDTLEDLFDKSTESDISDLVDNASVDQGNSLQLFQQQEQTESEEQLQQLKRKYFQSPEQKALLSLSPQLEAISISSRTSKKAKKQLFGDDSGIELSHHEADDSVAEGEKQVETESEDSSLKPGQNGAEALFRAKNKRAFMLAKFKDGFCVSFMDLVRVFKSDKTCSVDWVVCGLYLIEERTEAAKVLLQDYCEYIYFNQIGLVCLYLLSFKSQKSRETVQKLLTKLFDCKDDQLMLEPPKTRSTAVALFWYKKSMVSSVYTYGVLPDWIAKQTLLTHQFAGEKPFDLSRMVQWAYDNEYFEESEIAYYYACLAETDENAIAFLSSNNQAKHVRDCATMCRYYKRAEMQKMTMSHWIYQRCQAAKGEGSWKEIVKFLRHQTIEFISFLADFKKFLRGTPKKNCLVFWGPPNTGKSLFCMSLLNFLKGRVISFVNSRSQFWLQPLSDTKIGLLDDATRPCWDYFDTYLRNALDGNPISIDCKHKAPFQIKCPPLMVTTNVNVWGDERWKYLRSRISVFCFASQFLFDEDGSPGFVLNDKSWASFFERFWTHLELSDQEEEGEDGESQQGLRLFTRGTSESV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should emphasize Basic and acidic residues compositional bias.2. The designed protein should contain at least one Nuclear localization signal, Nuclear export signal motif to achieve its intended function.3. The ATP binding site should be stable and able to withstand changes in environmental conditions.4. The protein must exhibit the following characteristics: The E1- E2 complex binds to the replication origin which contains binding sites for both proteins; ATP-dependent DNA helicase required for initiation of viral DNA replication; Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer; It forms a complex with the viral E2 protein.5. The designed protein should be involved in DNA replication by localizing to host cell nucleus and exhibiting ATP binding, ATP hydrolysis activity, DNA binding.6. The designed protein should contain one or more SF3 helicase domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MADDTGTEEGLGCSGWFSVEAIVERTTENTISDDEDENVEDSGLDLVDFVDDSRIIPTNQLKAQALLNRQQAHADKEAVQALKRKLLGSPYESPASDLQESINKELSPRLGGLQLCRGSQGAKRRLFQSLENRDSGYGYKFKDAFAVSFGDLTRCYKSNKTCSQHWVVTVFGARENLVEAAKEVLKPQCNYLQETVGWAGNSRLSLFLFEFKVQKSRETLSKQLSAILGIDELLIMAEPPNHRSTLAAFFFYKKTLFSGENTTYYGQRPEWVAKQTILEHKFKEQFGLGYNELVRHFKSSRTACVDWVVCVFGVYCTVAEGIKQLIQPLCEYAHIQVLPCQWGMTVLMLVRYKRAKNRETVAKGLSTLLNVPESHMLIEPPKLRSSPAALYWYKTSMSNISDVYGETPEWIVRQTMVGRFKESYGLGFGELTRTFKSNKTCNPDWVVAAHGVHPTVYESMPAVLRSSCEYMLITGNCGKGGYLVLMLLRFRAHKCRVTLAKMLKDKFTIDEIQIMSEPPRVKSVPAALFWFRLALSKTAHVGGETPEWITRQTLVSHKFKEQFGLGYNELVRHFKSSRTACVDWVVCVFGVYCTVAEGIKQLIQPLCEYAHIQVLPCQWGMTVLMLVRYKRAKNRETVAKGLSTLLNVPESHMLIEPPKLRSSPAALYWYKTSMSNISDVYGETPEWIVRQTMVGKFKDVYGVSFSELVRCFKSDKTSCIDWVVAAAGVHHSVAESLKQLIQPFCHYAHIQCLTCNWGVYLLMLTRFKCAKNRATVAKCLSTLLNVQEQYMLIEPPKLRSPAAALYWYRTGISNVSEVIGETPEWIVRQTMLQ",
    "response#2": "MADPEGTEEEGTGCNGWFYVQAVVDRTTGHCVSEDEDEDEDDVQDSGLDMVDFIDNSLISSGSLGERAEAQALLNAQEREADVEAVQRLKRKYVGSPYVSPLQTSEPGIDNISPRLEAISIGQRPRGAKRRLFQQVNFISFLNDLKAFLKGKPKHNCILIHGPPNTGKSLFCMNLLNFLGGNVLSFANSKSHFWLQPVIDAKIVLIDDATFACWDYMDTYLRNALDGNPICIDRKHRLPVQTKCPPLLVTSNINILTNERWRYLYSRVRCYGFSVDMPLDRNGNPEGTDPLDDCGGFLDTEADCLDCDNLEEDLTELFDADTVSSLLDDTDQVQGNSLEPFQHHEATETLKSIEHLKRKYVDSPDKSLGIDNSVNALSPRLQAFSLSGQKKAVKKRLFGTDGDEEENFSLSVMIQWAYDNEIFEEPEIAYKYAQLADQDANAAAFLNSNCQVRYVRDCAQMVKLYKRQEMKEMSIASWIEKCCEKCKEESSWKPIALFLKHQNIPFVQFLITFKTWLKGLPKKNTLVIHGEPDTGKSYFTFSFLHFVQGKVISYMNKTSPFWLMPLLDTKFGLMDDATYNAWLYIDQNMRNALDGNPMCLDSKHKAPTQLKLPRMLITTNINVLKETTLLYLHSRVLCLEFSNKMPFNTDGSPLFPITDATWKCFFRKFGEQLDLTASEDEGNGDSGDLDRPFRFYA",
    "response#3": "MADSKGSTSKEGLSDWCILEAECSDLENDFEQLFEQDTDSDVSDLLDNGELEQGNSLELFHQQECEQSEEQLQILKRKYLSPKAVAQLSPRLESISLSPQQKSKRRLFAEQDSGLELTD"
  },
  {
    "reference": "MSRKKAVILVSGGLDSTTVIAIARSQGYDCYTISFDYGQRHRAELKAAEITAKAHGSLEHKVIRLDLGAIGGSALTDTSIDVPEEETSGIPVTYVPARNTVFLSIALGWAEVLGALDIFIGVNAVDYSGYPDCRPDYIAAYETMANLATKRGIEGERLHIRTPLINLTKAQIIQQGLALGVDYRLTVSCYQANDGGEACGKCDSCRLRKQGFEQAGVADPTRYAF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be designed to have high stability and activity towards 7-carboxy-7-deazaguanine + ATP + NH4(+) = 7-cyano-7-   deazaguanine + ADP + H(+) + H2O + phosphate. The designed protein sequence is ",
    "response#1": "EQHGKAP",
    "response#2": "GAAE",
    "response#3": "MSII"
  },
  {
    "reference": "MCHFIEGKNVTLGVTGGIAAYKSIELLRLLTKAGARVRVIMTENACWFVGPGTFQALSGQPVCTSVFDEGDAAIRHIDWAQSTDLAVIAPATANCIGKLAGGIADDALSTYMLAVTAPVVVCPAMNTNMYQHPSVQRNLEILRGYGHTLVDPDAGQLACGTVGPGRLPEPEFIMEKIVAVMASNDFAGRTILVTAGPTREAIDPVRFISNPSSGKMGFAIARAARQRGATVNLVSGPVDLPDPVDVAVHRVTSAQEMHDCVLQLLGDADIVIKTAAVSDYRPSSPAAHKIKKSAAKMTLEVEKTRDILAEIGQQKGKRLIIGFAAETQDLKNNAVQKLKAKNLDLIVGNIIGAEGSGFAGDTNRVTFFYPDGMAEPMDVMTKDKTAHCLLDRIAALKTRVS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be able to bind CTP ligand in a variety of conditions2. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.3. The protein contains novel DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains that confer a unique function or activity.4. The Proton donor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.5. The designed protein should be optimized for phosphopantothenate--cysteine ligase activity, metal ion binding activity in order to improve coenzyme A biosynthetic process, pantothenate catabolic process.6. A protein that can modulate the overall flux of metabolites through the Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MNVLIGVTGGVAAYKVCEVVSTLAKSGASVRVMMTARSQDFVSALTFAALARNAVYTDVEFWSAQQGRPLHIELGEWADVLAITPLTANTLGKLAHGLADNLLTNTVLASVCPVLLAPAMNTDMWQQQSVQRNWEGLRQDARYHTVGPGAGRLACDRIGTGRMAEPVEIVAQIDELLGAPGDLLAGRHVLVSAGGTREYLDPVRFLGNRSSGLQGYALARAAAARGAEVTLVAANVSLPDPAGVKVVRVETTAQLRDAVLAGAPAADAIVMAAAPADFRPTDVSDAKIKKSEDGSAPAITLEQNPDILKEISQHRLREGQVIVGFAAETGDATGSVLELATAKLARKGCDLLVVNDVSGGAVFGSPDNEAVILFDDAGEAERIPRGSKSALAHAVWDTVRARL",
    "response#2": "KRVLLGVSGGIAAYKTPELVRRLKEQGADVRVVLTQGAKAFVTAMSLQAVSANPVHDNLLDPAAEAAMGHIELAKWADLIVIAPASANLLARLTHGFADDLLTTLCLASTAPIYVAPAMNQQMWAAAATQANMAQLSARGVITIGPDSGEQACGDVGAGRMVQPDAIVAFLGRRLVPRDFILAGKNFMVTTGGTREKIDPVRYVGNNSSGKQGIAIARAAASRGAAVSLVAANVDSNLLASLDPKIHIQRVANADEMKLTVWASAAEMDVVVMAAAVADFRPKSVSQSKIKKNSADGLTIEFESNVDILASLCSARSQLKSGALLVGFAAETGDERSPLELAKEKIQRKPVDLLILNDVSGGQVFGEDSNQATLLFADGSEAPVDRGSKSALAHAIWDHVLNL",
    "response#3": "MNVLIGVTGGVAAYKVCEVVSTLAKSGASVRVMMTARSQDFVSALTFAALARNAVYTDVEFWSAQQGRPLHIELGEWADVLAITPLTANTLGKLAHGLADNLLTNTVLASVCPVLLAPAMNTDMWQQQSVQRNWEGLRQDARYHTVGPGAGRLACDRIGTGRMAEPVEIVAQIDAVLRPHQPFVGKKVLITAGGTREALDPVRYLTNRSSGKMGYALAQAAVENGAHVFLITTVDRGDIFGVKKIQVDSTQDMYKETRRLFPDMDIFIGAAAVSDFKAAHLAEHKIKKQSDTGLILELTQNPDILKAVGYDKTEQQIVVGFAAETQNIVTYGQDKLAKKHADMLVVNDVGQADSGFSSDNNAVTIVDADGRVDDLPRASKRDVADAVLDHVTRLLPSGGL"
  },
  {
    "reference": "MPDTINKINLLDYSPEGLKDYFSSIGEKPFRATQVIKWLHQMSVDDVGQMTNLSKGLRSFLQENAEIRAPEIIMDQHSVDGTHKWVLRLHDGNAIEMVYIPEDDRGTLCVSSQVGCALDCTFCSTARQGFNRNLTVAEIIGQLWIAKRTLQSDPKGARVVTNVVMMGMGEPLLNFEPLISALSLMMDDNAYGLSRRRVTVSTSGVLPALDKLGDRLDVSLAVSLHAPNNALRDQLVPINKKYPIAQLLAGCQRFLDKKTTQRKYITWEYVMLEGVNDKDEHAHELAKLLKDIPSKINLIPFNPFPDTRYKRSSNNRIYRFRDILINAGYTVMTRKTRGDDIDAACGQLAGEVTDRSRREIHFARIEKAIIK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.2. The protein must be engineered to have a cooperative binding mechanism with [4Fe-4S] cluster to ensure proper regulation.3. The designed protein must be optimized for tRNA binding, metal ion binding, 4 iron, 4 sulfur cluster binding, rRNA (adenine-C2-)-methyltransferase activity, rRNA binding.4. The protein's S-methylcysteine intermediate must be carefully engineered to ensure that the amino acids involved in adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "MKKQRITDLPASAFPELIAELGAPAYLAKELKLWIYSKKVSSFEEMSNFPKAVREQLAERFDIDCATVFAVQVSEDGTKKFLCALEDGKEVECVLIPADKGRRTVCISSQVGCAMACDFCRTAKMGLKRHLILGEITSQLRIAAREADTPITNVVFMGMGEPLHNLDAVVEATKLMYDQDAFGLSKRKITVSTSGLIPKMKEFVARSEVKLAISLSATTDEMRDKLMPVNKRYPLKDLMAFCRWYSDEYKHRLTFEYILIEGVNDHKEDAIRLVTLLEGVRAKVNLIPMNPYKEVAYYPTSRERMYWWQNYLDSKGVSTTIRISRGQDILAACGQLARGSDTRANLFDLSLAECEALAVEMGVKTFHGRNLFKWLHKHGITDFDAMTDLPKALRDRLRDTVRVELPRIVATHPSADGTIKWVMALHDGQRIETVCIPEGKRRTICVSSQVGCALECAFCATARQGFNRNLTVSEIIGQVWHAARELAQPPTNVVMMGMGEPLANFDAVVKAMDIMQDDLAYMLAKQRVTLSTSGIVPNIYRLRAVSDVSLAVSLHAPNDALRDQLVPINQKYPLAELIPACKAYIGHDKRRKITWEYVMLDGVNDSLIHAKQLIRLLEGVPSKLNLIPFNPFTGSDFCTSPMERIEAFRLRLTGAGIFAMTRKTRGDEIAAACGQLVGRVEDRTRDQVGELVK",
    "response#2": "MKKQRITDLPASAFPELIAELGAPAYLAKELKLWIYSKKVSSFEEMSNFPKAVREQLAERFDIDCATVFAVQVSEDGTKKFLCALEDGKEVECVLIPADKGRRTVCISSQVGCAMACDFCRTAKMGLKRHLILGEITSQLRIAAREADTPITNVVFMGMGEPLHNLDAVVEATKLMYDQDAFGLSKRKITVSTSGLIPKMKEFVARSEVKLAISLSATTDEMRDKLMPVNKRYPLKDLMAFCRWYSDEYKHRLTFEYILIEGVNDHKEDAIRLVTLLEGVRAKVNLIPMNPYKEVAYYPTSRERMYWWQNYLDSKGVSTTIRISRGQDILAACGQLARGKCNLKTLSFHELELILAEMGEKTYHARQISRWVYQRGVADFAAMTDISQSLRRKLDEGYYISELPLVEVRTGEDGCSKYVFRLPEGLDIESVLIPDQGRLTLCISTQLGCRQGCRFCLTSRMGFIRNLLAHEICDQVLQVRKRLPEEKRNLNIVLMGMGEPLDNYQQTISAIRLMTSPLGLNISPRHITLSTAGLVEELYRLRQEKLGINLAVSINAPFEDLRSWLMPVNRRYSLESLLKALRDFPLPARQRFTIEYILIGGVNDSPEAARQLCRLLRGIPCKINLIPYNPNPFLNFRECTEKSLEQFQQILIQHHLTATVRRSRGRDIGAACGQLGYVRGREDLIGLSREQLAGRLTAMGEKSFRAGQVWAWLYNRGIRDIDGMSNISKETRAKMRERFHISRPQVSRELTSADSTRKWLLKYRDGQEAETVYIPDDDRGAVCVSTQVGCTLTCRFCHTGTQMLVRNLSAGEIVGQFMVARDSYGEWPTPNETGRLLSNVVVMGMGEPLFNYDATVTALRILSDPEGLALSKRRITLSTSGVVPRMYDLGNDVGVNLAVSLHAATDDVRDVIMPINKKWPLKELMQACRDYPSASNARRITFEYIMLKGINDSDADARQLARLVKGIPCKFNLIPFNKWPGSDFECSDWSRIERFSDILAEQGYSAPVRKPRGRDILAACGQLKSESQRERL",
    "response#3": "MKKQRITDLPASAFPELIAELGAPAYLAKELKLWIYSKKVSSFEEMSNFPKAVREQLAERFDIDCATVFAVQVSEDGTKKFLCALEDGKEVECVLIPADKGRRTVCISSQVGCAMACDFCRTAKMGLKRHLILGEITSQLRIAAREADTPITNVVFMGMGEPLHNLDAVVEATKLMYDQDAFGLSKRKITVSTSGLIPKMKEFVARSEVKLAISLSATTDEMRDKLMPVNKRYPLKDLMAFCRWYSDEYKHRLTFEYILIEGVNDHKEDAIRLVTLLEGVRAKVNLIPMNPYKEVAYYPTSRERMYWWQNYLDSKGVSTTIRISRGQDILAACGQLARGTNLLGFDLEGLAAYCEQLGEKRFRATQLFRWIHQKGASDFAQMSDLAKSLRDKLATRAHIQALPVISEQASSDGTIKWLFDVGQGNAVEAVFIPEDDRGTLCVSSQAGCAVGCRFCSTGHQGFSRNLETWEILAQLWFAEHHLRERLNTPDRVISNVVMMGMGEPLQNYAALVPALKVMLDDHGYGLSRRRVTVSTSGVVPMMDRLREDCPVAMAVSLHAPSDPLRDQLVPLNKKYPIAELLAACNRYLDAAPRDFITFEYCMLDQVNDTPEQAEELVRLLKGKVPCKLNLIPFNPFPASGLKRSPKERVQAFAEVLIRAGIVTTVRKTRGDDIDAACGQLAGEVQDRTRAIERNGALFLEGREDLIGLSREQLAGRLTAMGEKSFRAGQVWAWLYNRGIRDIDGMSNISKETRAKMRERFHISRPQVSRELTSADSTRKWLLKYRDGQEAETVYIPDDDRGAVCVSTQVGCTLTCRFCHTGTQMLVRNLSAGEIVGQFMVARDSYGEWPTPNETGRLLSNVVVMGMGEPLFNYDATVTALRILSDPEGLALSKRRITLSTSGVVPRMYDLGNDVGVNLAVSLHAATDDVRDVIMPINKKWPLKELMQACRDYPSASNARRITFEYIMLKGINDSDADARQLARLVKGIPCKFNLIPFNKWPGSDFECSDWSRIERFSDILAEQGYSAPVRKPRGRDILAACGQLKSESQRERL"
  },
  {
    "reference": "MKTFILLAVLGLASASVHQHKIVWRESKKMGMIRTGQYPAYLEYQRNLRAVSPNVLANLPQNVNDFGDFEYLGNITIGTPDQGFIVVLDTGSSNLWIPGPTCKTNCKTKSKFDSTASSTFVKNGKSWTIQYGSGDAAGILGQDTVRFGAKGDSQLSVPTTTFGIASKISADFKNDATDGILGLAFTSLAVDGVVPPLINAINQGILDQPLFSVWLEHRGAANNVGGGVFTYGAIDTTNCGALVAYQPLSSATYYQFKAAGFKLGSYSNTKTVDVISDTGTSFLGGPQSVVDGLAKAAGATYDDFNEVYFIDCAAQPGTLDITIGTNTYSIQPVNYIVDAGNGQCLFAAFPFDFGGFGPSWILGDPFIRQYCNIYDIGNKRMGFAPSLQK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "RGFGGAMTDAAAINILSLSAGAQEQLLQQYFSSEGIGYSVVRVPMASCDFSTRLYTYADTPGDYNLVNFTLAPEDVDMKIPLLKRAQAMSPQPLHLLASAWSAPAWMKTNGALVGKGSLKGQPGGKEHKAWAQYYIRFLEEYAKYNLTFWALTTGNEPSAGLITNYSFQALGFTPEEQRDWVALDLGPAVRSSSFPQTHILILDDNRLLLPYWATVVLSDVHAARYIHGVAVHWYLDSFVPAEISLGSTHHLYPEYYLFGTEACAGWSTGDRGVKLGSWERAEQYAHDIIQDLNQYVVGWTDWNLALDQAGGPNWVKNFVDSPVVVDAQRDVFYKQPTFYSLAHFSKFL",
    "response#2": "RGFGGAMTDAAAINILSLSAGAQEQLLQQYFSSEGIGYSVVRVPMASCDFSTRLYTYADTPGDYNLVNFTLAPEDVDMKIPLLKRAQAMSPQPLHLLASAWSAPAWMKTNGALVGKGSLKGQPGGKEHKAWAQYYIRFLEEYAKYNLTFWALTTGNEPSAGLITNYSFQALGFTPEEQRDWVALDLGPAVRSSSFPQTHILILDDNRLLLPYWATVVLSDVHAARYIHGVAVHWYLDSFVPAEISLGSTHHLYPEYYLFGTEACAGWSTGDRGVKLGSWERAEQYAHDIIQDLNQYVVGWTDWNLALDQAGGPNWVKNFVDSPVVVDAQRDVFYKQPTFYSLAHFSKFY",
    "response#3": "RGFGGAMTDAAAINILSLSAGAQEQLLQQYFSSEGIGYSVVRVPMASCDFSTRLYTYADTPGDYNLVNFTLAPEDVDMKIPLLKRAQAMSPQPLHLLASAWSAPAWMKTNGALVGKGSLKGQPGGKEHKAWAQYYIRFLEEYAKYNLTFWALTTGNEPSAGLITNYSFQALGFTPEEQRDWVALDLGPAVRSSSFPQTHILILDDNRLLLPYWATVVLSDVHAARYIHGVAVHWYLDSFVPAEISLGSTHHLYPEYYLFGTEACAGWSTGDRGVKLGSWERAEQYAHDIIQDLNQYVVGWTDWNLALDQAGGPNWVKNFVDSPVVVDAQRDVFYKQPTFYSLAHFSKFL"
  },
  {
    "reference": "MQYLPIFLNIRGQQCLVVGGGTVAVRKVALLRQAGAEVKVIAPKLHEQLQEWADKGKITAQQASFSETEIKSCYLVIAATDDSSLNEQVYHLATAQGVLVNVADCPRFCDFILPSIVDRSPVIVAVSSGGASPVLARLLRARLETLIPQAYGRLGQFAARYRSRIKQRITGIRARRIFWEKVLQGSIAERIFAGQEEEAEGALEAALEGGFVPEQGEVYLVGAGPGDPDLLTFRALRLMQQADVVFHDRLVSPEVLALVRREAERIYVGKKRSWHAVRQEEINMMLVRSAREGKRVLRLKGGDPFIFGRGGEEIATLAAENIPFQVVPGITAASGCASYAGIPLTHRDHAHACIFVTGQLKEGRLSLNWQALVQPQQTIVVYMGLSGLEILCQELIAHGMPAVTPAALVQQGTTSRQRVLTGTLATLPDIVQGEDIHAPTLVIIGGVVALYPQLAWFKVPDREPLDAR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have NAD binding, precorrin-2 dehydrogenase activity, uroporphyrin-III C-methyltransferase activity to facilitate methylation, siroheme biosynthetic process.2. The protein should be able to bind NAD(+) ligand in a variety of conditions3. The protein must exhibit the following characteristics: Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1.4. A protein that is capable of catalyzing reactions within the siroheme biosynthesis pathway with high efficiency and specificity.5. The protein have enhanced 2 H(+) + siroheme = Fe(2+) + sirohydrochlorin. The designed protein sequence is ",
    "response#1": "GEVSLVGAGPGDAGLLTLKGLQRIQQADVVLYDALVSEQVLDLVRRDADRISVGKRAGNHSVQQEETNRLLVKYAKQGKRVVRLKGGDPFVFGRGGEELEVLKDAGIAFEVVPGITAALGATAYAGIPLTHRDHAQTAMFITGHTKPDGKNLTWNTLAQGNQTLAVYMGTIKAAELAEQLQAHGRKPNTPVAVISHGSLPTQKIYSGELKDLPKLAENAASPALIVVGETVALVGAGPGDPGLLTLKGLQQIQQAEVVLYDQLVSPEILDLVRRDATLISVGKKAGDHSVPQDETNRLLVQYAKAGNRVVRLKGGDPFMFGRGGEELEVLADEGIPFSVVPGITAAAGATAYAGIPLTHRDHAQSAVFITGHCQKAGKEPDWQQLAATNQTLVIYMGLMRSEHIQQQLIDHGRQPQTPIAIIERGTQTRQKVLTGTLGELATLARHAASPSLIVIGEVLAAS",
    "response#2": "VGAGPGDPDLLTFRALRLMQQADVVLYDRLVAPAIIEMCRRDAERIYVGKRRADHSVPQEQINQLLVDLARQGKRVLRLKGGDPFIFGRGGEEIEELAEQGIPFQVVPGITAASGCSAYAGIPLTHRDYAQSVRFVTGHLKDGTSNLPWNDLVAPAQTLVFYMGLVGLPTICSELIRHGRAADTPAALVQQGTTKNQRVFTGTLADLPQLVAEHEVHAPTLVIVGEVAACCASS",
    "response#3": "VGGGPGDPDLLTFRALRLMQQADVCVYDKLVSKEVMALVRRDAELIYVGKSRDQHTLPQEDINQLLVRLAKEGKRVLRLKGGDPFIFGRGGEEIETLMENGIPFQVVPGITAANGVSSYAGIPLTHRDYAQSCLFTTGHLKDGSVNLDWDALVRPNQTVVIYMGLVGLPEICRQMVAHGAPENLPIAIVQQGTTQRQRVVEGTLATLPGLVERAGLRAPCLIIVGQVGEVYLVGSGPGEPDLLTFRALRLMQRADVVLYDSLIAPAILALVSPEAERIHVGKRAAEHTLPQDDINALMVALAREGKRVLRLKGGDPFVFGRGGEEISELAAAGIPFQIVPGITAANGCAAYAGIPLTHRDCAQSVTFVTGHLKAGALNLSWAELAQRGQTVVFFMGRRNLATICARLIEHGLPGDWPAAMIIDGTTARQRLIAATLDDLPDRVAAERSTGAALLIVGEVVRLVGGGPGDPDLLTFRALRLMQQADVVLYDRLVSPAVLELVRRDAERINVGKARSDHTLPQEDINTLLVRLAKAGRRVLRLKGGDPFIFGRGGEEIETLAAEGIPFQVVPGITAASGCASYAGIPLTHRDHAQACVFVTGHMKHGVLDLNWTALAQPRQTIVFYMGLHGIEQIRDGLVGHGLSPDTPAAVVEQGTTAQQRVHVATLATLPERVRDAGIRPPSLIIVGSVL"
  },
  {
    "reference": "MCFLAGKHWLCKHCGNIAHVLMSLEITMKLTLPDYQKADVLVVGDVMLDRYWYGPAGRISPEAPVLVVKINQIEERPGGAANVAMNIAALGGKARLIGIVGQDEQANILNTILTSLNVDCNFIRLAELPTITKLRVMSQNQQLIRLDFEEDFHSVNFAPILEQIEMALLHTKVMILSDYAKGTLANVQSMIQLARSAGIPTLVDPKGANFEHYRGATILTPNISEFEAVVGACLTVEDIVTKGLKLIHQLEFDALLVTRSDHGMMFLRASHEPLSLPTLAKEVYDVTGAGDTVISVLASSLAAGKSMCEACALANAAAGVVVGKLGTSTLSTIELTEAIHGSHQSGFGVVTEEPLIAAVKIAKARGEVVVMTNGCFDILHAGHVAYLNKAARLGDRLIVAVNTDESVHGLKGAGRPVNPADRRMAVLAGLSAVDWVVSFSEKTPQRLISKILPNLLVKGGDYKPEDIAGSKEVIAAGGNVLVLNFEGGCSTTEIINSILSGHNS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The design of the protein must focus on the precise arrangement of amino acids in the active site to enable effective catalysis of ATP + D-glycero-beta-D-manno-heptose 7-phosphate = ADP + D-   glycero-beta-D-manno-heptose 1,7-bisphosphate + H(+).2. The protein contains novel Cytidyltransferase-like, Carbohydrate kinase PfkB domains that confer a unique function or activity.3. The ATP binding site should be stable and able to withstand changes in environmental conditions.4. The designed protein should have phosphotransferase activity, alcohol group as acceptor, heptose 7-phosphate kinase activity, heptose-1-phosphate adenylyltransferase activity to facilitate ADP-L-glycero-beta-D-manno-heptose biosynthetic process.5. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7- phosphate at the C-1 position to selectively form D-glycero-beta-D- manno-heptose-1,7-bisphosphate.. The designed protein sequence is ",
    "response#1": "MPRFDGAQILVVGDLMLDRYWQGPTSRISPEAPVPVVKVEQLEDRVGGAGNVALNIAALGAGASLVGVVGADEAAHSLETRFSSAGIRTDFQVSDDKPTITKLRVISRHQQLLRLDFEQQFTASDTDQIADKVAASLANVSVLVISDYGKGALQNPVELIQAARQHGIPVLVDPKGSDFSRYQGATLITPNMGEFEAVVGPCESEAAIVEKGLALLEELDLEALLVTRGEHGMTLLRQGMPEFHLPARAREVFDVTGAGDTVISVLAAALGAGEALPAAVVLANLAASIVVGKLGTATISAPELRRALDVERQRGRTIVFTNGVFDILHAGHVHLLRQAKQLGDVLVVGVNSDQSAQRLKGKHRPINSERDRLALVAALDPVDYVALFDEDTPATLIRTLRPHIHVKGGDYADEQLPEAGAVHEVGGRIVIIPLVGSMSTSSVIDRILSGGGTS",
    "response#2": "MPRFDSAPVLVVGDVMLDRYWHGPTRRISPEAPVPVVKVDQIEDRPGGAGNVALNIAALGAPAWLVGVTGDDEAAAKLSQRLEAAGVYCAFQKHPEQPTITKLRVMSRHQQLIRVDFEEGFNTDAPALLAEVSRLLDGVKVMILSDYGKGALVNHQALIAVARERGIPVLADPKGQDFSIYRGASLITPNLSEFEAVVGHCNSEQQLVEKGLALIDELQLEALLVTRSEQGMTLLRKGEAPLHLPARAREVFDVTGAGDTVISTLATALAAGEDYPQAVALANLAAGIVVGKLGTATVSAPELRRAVARWRRWGLKIGFTNGCFDILHPGHIAYLTQAKGWCDRLIVGLNTDRSVRAAKGRGRPVNDLDARALVLAGLACVDLVVPFDEDTPQALIDAARPDILVKGGDYRIEDVVGHGAVTGYGGELRLATFVEGHSTSATLRRIARAKAHGEKIIMTNGCFDLLHAGHVTYLEQARSLGDRLIVAVNSDASVKQLKGDSRPINGLQARMTVLSALACVDWVVPFSEETPERLYCKLLPDVLVKGGDYTPDQVAGGDCVIQAGGEVRILQFVDGQSTTAMINRARG",
    "response#3": "MPRFDSAPVLVVGDVMLDRYWHGPTRRISPEAPVPVVKVDQIEDRPGGAGNVALNIAALGAPAWLVGVTGDDEAAAKLSQRLEAAGVYCAFQKHPEQPTITKLRVMSRHQQLIRVDFEEGFNTDAPALLAEVSRLLDGVKVMILSDYGKGALVNHQALIAVARERGIPVLADPKGQDFSIYRGASLITPNLSEFEAVVGHCNSEQQLVEKGLALIDELQLEALLVTRSEQGMTLLRKGEAPLHLPARAREVFDVTGAGDTVISTLATALAAGEDYPQAVALANLAAGIVVGKLGTATVSAPELRRAIHADRAGRGVLSEAQLARLVARWRRWGLKIGFTNGCFDILHPGHIAYLTQAKGWCDRLIVGLNTDRSVRAAKGRGRPVNDLDARALVLAGLACVDLVVPFDEDTPQALIDAARPDILVKGGDYRIEDVVGHGAVTGYGGELRLATFVEGHSTSATLRRADARAHGENIVFTNGCFDILHAGHVGYLEQARGLGQRLIVAVNADESVRRLKGEGRPINSEDRRMAVLAGLESVDWVLVFGDDTPERLLELVRPDILVKGGDYKKEEVVGWEIVEAYGGKVQALDFLDDCSTTAIVEKIRS"
  },
  {
    "reference": "MTLPAWLDPLFDAGEMGAADSFASEERGVPSLELMERAGEGLARITAEAADAGGRPGPIRIVVGRGNNGGDGLVAARVLRERGREVDVLSTAALGDLGGDARANLDRLPGDPPAEGVCEESLRGSAAVLDALLGTGFSGEPREPAASAIAAMNAQDSAVVACDVPSGVDASTGAVAGEAVRAAMTATFHAPKIGLHVAPGALHAGTVHTVEIGIPRDAPSATTAGLISDRVLALVPARARAGSKFDAGTVVIAGGMRGLTGAPTMAALAAMRTGAGYVQLAVPESTETVFALKLLEAMTAGQPEADGAHSEAGADGVAELAERAGAVVLGPGIGRSEGAVAFARAVARRVQAPLLIDADGLNAHAGALEALRERPAPTVLTPHAGELGRLLETDSSAIGERRLSSAREAAARSGAIVVLKGDDTIVAAPDGLAAVSRGGSPALATAGTGDVLCGVIATYLAKGLDALPAAAAGVLAHARAGRVAASRFGADHTIASDVIDSLSAALGGPARARERRAAEGIESGRPTR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be able to bind (6S)-NADPHX ligand in a variety of conditions2. The protein should be able to modulate nicotinamide nucleotide metabolic process in a way that leads to a desirable outcome.3. The protein should have a low Km value for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "PYSKKGDSGRMLIIGGSNIYHGAPILAGLAALRSGVDLVYLYLPEKIASIARSLAPEFIVIPYKDEEFNEKSAKEIIEFIEKVDAVLIGNGLGLGHDDAVEIIFNAAEEKKKFLVADADALKTNAVKKARKIKAVYTPHAGEFKAITGIELPSYEEMNKRIEIIKEASKKLGVIFLVKGHYDIITDGEKVKISRGGTQAMTVGGTGDVLAGLCTGLIARTKLAFEASIAASYINKKAGELATKEYGNQMKATDLIEFIK",
    "response#2": "DKYTQGVTGVLAGSAAYPGAAILCAGAAVAATSGMVRYAGSAAAEVVDHWPEVVIAPSAAAAGRVQAWAVGPGLGTDETAAAALWFALDTDLPVIVDADALTMLAAHPDLVAGRTAPTVLTPHAGEFARLAGEPPGADRVGAARALADRFGATVLLKGHVTVIAEPGGGSVYLNPAGQSWAATAGSGDVLSGMIGALLAAGLPAGEAAAVAVFVHTRAAALAAADPGAGTAPASAL",
    "response#3": "VGIVAGSARYPGAAVLAVAGALSGGAGAVRYAGPATDAVIARFPETLVTAGPPAEAGRVQAWVIGPGLGDGSEARQALVDVLSSDVPVLVDADGLRLLDPQAVRARRAETLLTPHAGEAAALLGLSREEVEASRLSAVRALADRFGATALLKGSTTLVADGTRAIRVNPTGTPWLATAGSGDVLAGLTGSLLAAGMNARDAASVGAYLHGLAARALEGHKYARGHAVVVSGPAHATGAARLAARAALRAGAGLVSMAAEEDAIPTLSAHLTAIMIKKADGSDGLAELIADTRLNAVVIGPGAGVGEATCERVMACLNSEAAVVLDADAVTSFKEHPARLFDAIAARSAPVFTTPHAGEFSRLFPDLAEDENKVEKTRRAAERAGAVVILKGADTVVAAPDGRAAISAHDAPWLATAGTGDVLAGMIAGLAAQRMPAFEAACAAVWMHADAARRFGPGLISEDLPV"
  },
  {
    "reference": "MRDRFLRACRRQPVDRTPIWLMRQAGRYMPEYRAVRERYGFLQMVKTPEIAAEVTLQPIAAFGVDAAIIFADILPPLEGLGLRLTYEKGEGPVIHNPIRHPHDVATLHPCDPRETVAYTLDAVRLVKRELNGLPLIGFSGAPFTLASYAIEGGGSKEYRLTKRFMYEQPTAWHDLMERLGTLVAEYLIAQVEAGADAVQIFDSWAGTLAPADYRAYVLRHTQNVVATVRARLGAAAPPIIYFGTDMAGLAGELRQLGTDVLGVDWRIDLDVAWAQYGYEHAVQGNLDPMTLFAPPELIASRAREILQRAGGRPGHIFNLGHGILTETPVEHVRYLVEFVQSYPLPTTTPALQEVMQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that can modulate the overall flux of metabolites through the protoporphyrin-IX biosynthesis pathway, enhancing or suppressing its activity as needed.2. The designed protein should have a specific function within the cytoplasm.3. The protein should be able to bind substrate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "ISGLAASYHLIRGPSPPKVILVEGSKRLGGWIRSIRGSDGAIFELGPRGIRPAGALGARTLLLVSELGLESEVLPVRGDHPAAQNRFLYVGGTLHPLPSGLRGLLRPSPPFSKPLFWAGLRELLKPRGKEPDETVHSFAQRRLGPEVASLAMDSLCRGVFAGNSRELSIRSCFPSLFQAEQTHRSILLGLLLGAGQSPQPDSSLIRQARAERWSQWSLRGGLEVLPQALHNHLASKGVTVLSGQPVCGLSLQPEGRWKVSLGDSSLEADHIISAIPASELSKLLPAEAAPLARILSTIKAVSVAVVNLQYRGACLPVQGFGHLVPSSEDPTVLGIVYDSVAFPEQDGNPPSLRVTVMLGGYWLQKLKAAGHQLSPELFQQQAQEAAATQLGLKEPPSHCLVHLHKNCIPQYTIGHWQKLDSAMQFLTAQRLPLTLAGASYEGVAVNDCIESGRQAAVAVLE",
    "response#2": "ISGLAVAHHLRSRGTDAVLLESSARLGGAVGTHALAGYLVEQGPNSFLDREPATRALAAALNLEGRIRAADPAAKRRYVYTRGRLRSVPASPPAFLASDILPLGARLRVAGELFSRRAPEGVDESLAAFGRRHLGHRATQVLLDAVQTGIYAGDVEQLSVAATFPMLVKMEREHRSLILGAIRAQKAQRQAALPAGTAPKLSGALSTFDGGLQVLIDALAASLGDAAHVGARVEGLAREDGGWRLIIEEHGRRAELSVAQVVLAAPAHATAKLLRPLDDALAALVAGIAYAPIAVVHLGFDAGTLPAPDGFGFLVPAEEQRRMLGAIHASTTFPFRAEGGRVLYSCMVGGARQPGLVEQDEDALAALAREELKALAGVTARPSFTRVFRWPLGIPQYNLGHLERVAAIDAALQRLPGLHLIGNAYKGVGLNDCIRNAAQLADALS",
    "response#3": "NDTFLKACRGEKTDYTPIWMMRQAGRYLPAYQKIRGKVSFLELCKNPALCVEVTLQPVDLLGMDAAILFSDILILMEAMGAKLEFNEGCGPVFPNPIKDQTALDALIIPDADDATGFVMETIRLLRGELQVPLIGFAGAPFTCATYLIEGGSSKVFWETKKMMFTQPELFHGIMEKITQATILYLQAQARAGAQALQIFDSWAGVLAPCDFEVFALPYVRRIIASLQQFDLPIIYFANNGSTLLEMSASSGASVLGLDWRINIGDAGKRVPGIALQGNIDPFALLLPKDKLRKRIGTILEDAKEVKGHIFNLGHGIHQFTPPEQARIAVDAVHR"
  },
  {
    "reference": "MVSSVTNDSRSVAPSSVFIAIQGSGKDGHDFIDSAIASGAKAVIHARDISSFKNGISYIKVSDSYSAYARLVECFYDFPSSKMKILGVTGTNGKTSSVYLLNSILTSAGLKCGIITTVCYEWPGHSEPAARTTPEASEIQSHFSKMYEAGCTHVIMEVSSHALHQNRIGSAKFAGAIFTNLTGDHLDYHKTMEFYYDAKKILFQDHLAVDAVAAINTDDPFGKRLFADLRSANCIRFGRENCDAVIKINSISQLGTDFTLSIEGRTFKISSPLIGEHNAYNICGVAIIAYKLGISPEIIQSTLEKSIAIPGRLEAFHFKDNVSIFVDYAHSDDALRRVLEALTKLKKKRIICVFGCGGDRDRTKRPRMGAVSAKFADRTIVTSDNPRSEDPKEIIAEIVKGIPSTANFATVPDRGEAIEKALNEAEPGDIVLIAGKGHEDYQEIKGVKHHFDDREEVRKLIGSI",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should include a Meso-diaminopimelate recognition motif motif to ensure proper function.2. The protein have enhanced ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.3. The protein contains novel Mur ligase C-terminal domains that confer a unique function or activity.4. The ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate binding site should be located in a region of the protein that is accessible to the ligand.5. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..6. The protein design should consider the endogenous proteins and enzymes present in the Cell wall biogenesis, peptidoglycan biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MSLTSLISLLGPATESEPDGLRIASDSRQLRPGVAFLAYPGDQYDGRDFIADAIKRGAAAIIYESPYDLPVDYQNTEIPFVSVPHLSQCRAELAARFYGRPSEQLALVGVTGTNGKSTTAFMAQAILRAAGSPCGLLGTVQYDLCRRCVTADMTTPGPLDLAALLRECADAGAAAAVLEVSSHALEQRRADGLRFSAAAFTNLTQDHLDYHRTLEAYAAAKARLFEQLDASGVAVVNADDPRHERMLQGCRARVLRYSLERPAEIMAAITRDTIEGTLYRLRIDGRDLVLHNALVGRHNVYNAMAAAGLAGLATGVDAAAVRAGLESATGVPGRLERFTDSQGCQVVVDYAHTADALANALAALRPHVTGRLICVFGCGGDRDTGKRAEMAREAEQGADVVIVTDDNPRTEDPERIVRDIAAGFSGPGAYQVIHDRATAIAWAIDSVEPGDLVLIAGKGHETYQVVGAERRPFDDREVAREALRGAG",
    "response#2": "MKLQELAKELSVTLTGNGDIQITGLAVDSRKVKDGTLFAALPGSRVHGGEFIQYALRMGASAILTDAQGARIAADVLKGADAALVVTEDPRAALAFAARVYGDPSRQLQMIGITGTNGKTTSSYILENILEKTGHKCGLIGTIENVIGTEKIKASHTTPEALDLQKLFLEMKDAGADYCVMEVSSHALELNRVYGIDFQTAVFTNLTQDHLDFHKTMENYLNAKKKLFSQLGSKTYAIVNIDDPYGNRIVKESNAEVITYGIENKADLQATDIVSNEKGTKFTLHTGQSQFEVHLNIPGRFSVYNALTAIAICRHFGITEEQIKRGLLEVHVPGRFQYLISDNKITAIVDYAHTPDALKNVLETINSIRTKNEELITVFGCGGNRDKTKRPKMGHIASALSSKVIITSDNPRNEAPESIMEDIEKGIEPQNFKKVLSIADRKQAIKTACQMANPNDIILIAGKGHEDYQIIGKQKMHFSDREIVREMIENR",
    "response#3": "MKLSELFPGIRIASDSRQLRPGVAFLAYPGDQYDGRDFIADAIKRGAAAIIYESPYDLPVDYQNTEIPFVSVPHLSQCRAELAARFYGEPSKRLRLVGVTGTNGKSTTAFMAQAILRAAGSPCGLLGTVQYDLCRRCVTADMTTPGPLDLAALLRECADAGAAAAVLEVSSHALEQRRADGLRFSAAAFTNLTQDHLDYHRTLEAYAAAKARLFEQLDASGVAVVNADDPRHERMLQGCRARVLRYSLERPAEIMAAITRDTIEGTLYRLRIDGRDLVLHNALVGRHNVYNAMAAAGLAGLALGFPLETIVEGLSQAEGVPGRMNRLGGAAGLPLVVVDYAHTPDALEQALTALRAHCEGRLVCVFGCGGERDAGKRPQMGAIAERLADAIIVTDDNPRGEDGDAIVAQIVAGLRQPGEAVIERDRAEAIHRALASARAGDVVLIAGKGHEDYQIIGREKLPFSDREQARQALSSRTR"
  },
  {
    "reference": "MAGRRGLCVLLLLSALLAAADPTERSRTSSSKGRSFAGQKVPNTDNASEESYKVDDFAAKVLTGKLTTVFLPIVYVIVFIIGLPSNAMAIWVFFFRTKKKHPAVIYMVNLALADLLFVVWFPLKIAYHVNGNNWLFGEGLCKVLVGFFYGNMYCSILFMTCLSVQRYWVVVNPIVHSRKKFEIALGISLAIWILILLGSIPLYLVNQTAYISDLNITTCHDVLPNNILAHDMFNYFLSLAIGLFLIPALITAVAYILMIKTLNASISDISTGRKRKRAIKLIIAVLSMYLICFTPSNVLLVVHYLQLKNNNESSLYMSYITALCLSTLNSCIDPFIYYYISKDFRDNLKNALICRSVRTTRRMQVSLSSGRYPKKSNSYSSNSNGTTKSTY",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. Design the protein with a signal peptide incorporated.2. The protein should have Helical domains to ensure proper insertion into the membrane.3. The protein design must be able to perform thrombin-activated receptor activity, calcium channel inhibitor activity, protease binding in order to contribute to positive regulation of neutrophil mediated killing of gram-negative bacterium, positive regulation of leukocyte chemotaxis, establishment of endothelial barrier, positive regulation of phosphatidylinositol 3-kinase signaling. The designed protein sequence is ",
    "response#1": "GNALVVWVTAFEAKRTVNAIWFLNLAVADLLSCLALPILFTSIVKHNHWPFGDQACIVLPSLILLNMYSSILLLATISADRFLLVFKPIWCQKFRRPGLAWMACGVTWVLALLLTIPSFVFRRIHKDPYSDSILCNIDYSKGPFFIEKAIAILRLMVGFVLPLLTLNICYTFLLIRTWSRKATRSTKTLKVVMAVVTCFFVFWLPYQVTGVILAWLPRSSSTFQSVERLNSLCVSLAYINCCVNPIIYTIFNKEFRRAFAR",
    "response#2": "GNSLVMLVILYRRRTRSVMDVYVLNLAIADLLFSLTLPFLAVSKLKGWIFGTPLCKMVSLLKEFNFFSGILLLACISVDRYLAIVHATRTLARKRYLVKFVCVGIWGLSLILSLPFAIFRQAYKPFRSGTVCYEVLGEATTDFRMTLRGLSHIFGFLLPLLTMLVCYGLTLRMLFKTHMRQKHRAMGVIFAVVLVFLLCCLPYNLVLLSDTLLGAHLIEDTCERRNDIDQALYITEILGFSHSCLNPIIYTLRNKELRGALGK",
    "response#3": "GNSLVMLVILYRRRTRSVTDVYVLNLAIADLLFSLTLPFLAVSKWKGWIFGTPLCKMVSLLKEVNFFSGILLLACISVDRYLAIVHATRTLTRKRYLVKFVCMGTWGLSLVLSLPFAIFRQAYKPYRSGTVCYEVLGEATADLRITLRGLSHIFGFLLPLFIMLVCYGLTLRTLFKAHMRQKRRAMWVIFAVVLVFLLCCLPYNLVLLSDTLLGAHLIQDTCERRNNIDQALYITEILGFSHSCLNPVIYALCNATFKKTFKHLL"
  },
  {
    "reference": "MSQDQAPAKCPFSQASSAGRTNRDWWPKQLRVDLLHQHSSKSDPMGAGFDYAAEFKTLDLAAVKADLAALMTYSQDWWPADFGHYGGLFIRMAWHSAGTYRIGDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGRKISWADLMILTGNVALETMGFKTFGYAGGRPDVWEPDQDVYWGREDKWLGGDVRYTQGSPGVDKDRGVLVKDDDSKTPHTRDLENPLAAVQMGLIYVNPEGPDGNPDPVAAARDIRDTFGRMAMDDEETVALIAGGHTFGKTHGAAAASHVADEPEGAGIESQGFGWKNSHGTGAGADAITSGLEVTWTTTPTQWSNNFFENLFGFEWELTKSPAGAHQWVAKDAAEIIPHAFDKTKKQRPTMLTTDLSLRFDPAYEAISRRFLAHPDQFADAFARAWFKLTHRDMGPRARYLGPEVPAEVMVWQDPVPAVDHPLVDAQDVVGLKSKLLASGLTVAQLVSTAWASASTYRGSDMRGGANGARIRLAPQKDWAVNQPAQLAQVLAKLESIRTDFQAGGKKISLADLIVLAGAAGVEKAARDAGHAVEVPFTPGRTDATQEQTDVASFEPLEPVADGFRNYVKARYSVPAEVLLVDRAQLLTLTAPELTVLVGGLRVLGANVDDAQHGVFTDKPSQLTNDFFVNLLDMGTVWTESAGGAQPLYEGRDRKTGAAKWTATRADLVFGSNAQLRATAEVYAAADAQPKFVADFVQAWAKVMELDRFDLV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.. The designed protein sequence is ",
    "response#1": "AFARAWFKLTHRDMGPRARYLGPDVPTEELIWQDPIPAVDHVLVDDTDVAPLKETILASGLSVAELVSTAWASASTFRGSDKRGGANGARIRLAPQKDWAVNEPARLAKVLKVLERIQGEFNSTQPGGKKISLADLIVLAGGAGIEQAAKRAGHDVVVPFAPGRMDASQEQTDAHSFAVLEPVADGFRNFVKGKFAVPAEALLIDKAQLLTLTAPQMTALVGGLRVLNVQTGDEKHGVFTDQPETLTVDFFRNLLDMATEWKPIAGEDTYEGRDRRTGELKWTGTRVDLVFGSNAVLRALSEVYAN",
    "response#2": "TTSELVRTAWASAATFRGTDMRGGANGSRIRLAPQNKWDVNNPAELDKVLKRLEKIQKDFNGSQSGGKKVSLADLIVLGGAAAIEQAAXXAGQXVEVPFKPGRTDATQAQTDVDSFAVLEPTADGFRNYFGKGHSRSPADMLVDRASLLTLTVPEMTVLVGGMRALDANTGKSKHGVFTDRPGTLSNDFFVNLLDMSTKWSKAKTDGIYEGSDRKTGKLKWTATPVDLVFGSNSELRAIAEVYAAN",
    "response#3": "FGHYGGLFIRMAWHSAGTYRVFDGRGGAGQGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGNKISWADLLILTGNVALESMGFKTFGFAGGRPDTWEADEATYWGRETTWLGNDARYAKGFSGSDKRGSLIADEESHKTTHSRELETPLAAAHMGLIYVNPEGPDGNPDPVAAAHDIRDTFGRMAMNDEETVALIAGGHTFGKTHGAAPADNVGKEPEAAGLEAQGLGWANKHGSGKGPHTITSGLEVTWTKTPTQWNNNFLEYLFKFEWELTKSPAGAHQWVAKNADEIIPDAYDASKKHKPTMLTTDLSLRFDPAYEKIARRFH"
  },
  {
    "reference": "MELLKEQIREVARVEKGNVLKVDSFLNHQLDIPLLNEIGKELKKRFQGEKVDKILTAEVSGIAIAAIAAQYFNVPVVFAKKTQSKNLDQDTYEGEVYSYTKGQAYKIRVSKRYLHEKENILIIDDFLANGEALQGLREIVVQANANLVGAGIVIEKGFQGGGKRLRAENMRIESLAIIDEMNESTLVFRDDKADLKADPKAS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must be able to regulate xanthine metabolic process. The designed protein sequence is ",
    "response#1": "TYKNQCGRIGVIAGSKGFIGAALMTSQGALRAGAGLVEVFVPEEIYPIVAAAAPQEAMVKPVKSYRDLLREKIDVWALGPGLGKARAVEILEVIEKAKQPMVIDADGLNILADKISILKRCRGKRLLTPHPGEMKRLFPSKKETRAATATKFCNRFAVTLLFKGSRTIVARRDRPLSYNTTGNPGMASGGMGDILTGVCAGLVGQGLSLYDAARLGAWICGRAAEIL",
    "response#2": "VGETIYNKYKDEGITKVLTIEASGIAPAIMTALKFKVPCLFAKKSVPSTLTEEVYHTDIHSYTKNKTSHVVVSKRFLSENDRVVIIDDFLANGEAALGLYNLVQQAGATCVGVGIVVEKSFQSGKQRLLDAGLDVTSLCG",
    "response#3": "MKEIGKRFAQVYANAGVTKVVTIEASGIAPALYAAESLNVPMIFAKKAKNVTMNDDLLITEVYSFTKKLTSTVQISSKLIEEGDKVLIIDDFLANGQAALGLVHLMEQAKAEVVGLGMVIEKR"
  },
  {
    "reference": "MSQAGPKPGGLFFFFFMDSPNMSWYDGIYENRSLNESGPEPKPAHEYNYYAMLLTLLIFVIVFGNVLVCMAVCREKALQTTTNYLIVSLAVADLLVATLVIPWVVYLEVVGEWRFSRIHCNIFVTLDVMMCTASILNLCAISIDRYTAVAMPMLYNTRYSSKRRVTVMISVVWVLSFAISCPLLFGLNNTEENECIIANPAFVVYSSIVSFYVPFIVTLLVYVQIYIVLRKRRKRVNTKRVGTHLGPDSDTQAPLKDKCTHPEDVKLCTLILKTNGSFPLHKRKVDTVNQIDEMEMATSTSPPEKSKHKPSVPSHNELAMPAPSHMCANSTLRSPMDSPLQVAKNGHTTDNPKTAKAFEIQTMPNGKTQSSLKTISRRKFSQQKEKKATQMLAIVLGVFIICWLPFFITHILNMHCDCNIPQAMYNAFTWLGYVNSAVNPIIYTTFNIEFRKAFMKILHC",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should contain one or more G-protein coupled receptors family 1 profile domains that are essential for its function.2. A protein that contributes to regulation of long-term neuronal synaptic plasticity, response to xenobiotic stimulus, negative regulation of blood pressure, negative regulation of synaptic transmission, glutamatergic by functioning in non-motile cilium, axon, presynaptic membrane, dendrite with dopamine neurotransmitter receptor activity, coupled via Gi/Go, dopamine binding, G protein-coupled receptor activity. The designed protein sequence is ",
    "response#1": "GNTLVCAAVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSFCNIWVAFDIMCSTASILNLCVISVDRYWAISSPFRYERKMTPKAAFILISVAWTLSVLISFIPVQLNWHKARPLSSPDGNVSSQDETMDNCDSSLSRTYAISSSLISFYIPVAIMIVTYTRIYRIAQKQIRRISALERAAVHAKNCQNTTGNGANVECSQPESSFKMSFKRETKVLKTLSVIMGVFVCCWLPFFILNCMVPFCESDLPSGETKPFCIDSITFDVFVWFGWANSSLNPIIYTIFNHDFRRAFKKILC",
    "response#2": "GNVLVCMAVSRERALQTTTNYLIVSLAVADLLVATLCMPWVVYMEVVGEWRFSRIHCDIFVTLDVMMCTASILNLCAISIDRYTAVAMPMLYNTRYSSKRRVTVMIAVVWVLSFAISCPLLFGLNNTDEKECIIGNPAFVVYSSIVSFYVPFIVTLLVYVQIYIVLRRRRKRVSTKRSSHVC",
    "response#3": "GNSLVCVSVAAERALQTPTNYFIVSLAAADLLLALLVLPLFVYSEVQGGAWLLSPGLCDALMAMDVMLCTASIFNLCAISVDRFVAVAVPLRYNRQGGGRRQLLLIGATWMLSAAVAAPVLCGLNDVRGRDPAVCRLEDRDYVVYSSVCSFFLPCPLMLLLYWATFRGLRRWEAARRAKLHGRAPRRPSGPGPPAPAPGSPQDPCDPDCPPPAPGPSQDPCDPDCSPPDATLAATLPPQTQPQVPRRQRAKITGRERKAMRVLPVVVGAFLVCWTPFFVVHITRALCPACSVPARLVSAVTWLGYVNSALNPVIYTIFNHDFRRAFKKILCRG"
  },
  {
    "reference": "MENRWQALIVWQVDRMRIRTWNSLVKHHMYVSRKANKWVYRHHYENRHPKVSSEVQIPLGEARLVIKTYWGLQTGERDWHLGHGVSIEWRLKGYSTQVDPGLADQLIHMHYFDCFADSAIRKAIVGHIVIPRCEYQAGHNKVGSLQYLALTALIKPKKIKPPLPSIRKLVEDRWNSPQKIRGRRENHTMNGH",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Recruits an active E3 ubiquitin ligase complex composed of elongin BC, CUL5, and RBX2 to induce polyubiquitination of APOBEC3G and APOBEC3F2. A protein that localizes to host cell cytoplasm, host cell plasma membrane and can perform RNA binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "EWGRR",
    "response#2": "ETEKLA",
    "response#3": "EAEEPCEP"
  },
  {
    "reference": "MQRITPDQAHPLFDTAATRRQEQAMAAALPPHTLMQRAGRAVARLAQAIAPHARTVWIACGPGNNGGDGLEAATHLARSDRQVIITWLGSPEALPDDARKSWEKARNTAHITWSDTPPEHLTPQDLCIDALLGIGVRASTPPTPAPNTAGSVLAQWLAQLHTCTAPVLAVDVPSGLDADTGQYAHGLEAAASAGLTGGAGARYTLSLLTLKPGLFTAHGRDAAGQVWLDDLSAPPHPHTPHSRAQENQEPPTAWLAGPPARAQRHHASHKGSYGDVAIVGGEGLAQRGMGMTGAALLAASAALHAGAGRVLVSLLDPAETTEISTQPELMLRRFEALELEALTIVCGCGGGQAVRGVLPAVLQRAGTLVLDADALNALGAEGSLQQVLKARAARQHTTVLTPHPLEAARLLHTHTAQVQAHRLTAAQQLARQFQCVVVLKGSGTVIANPQGTPTINPTGNARLATAGTGDVLAGMIGAYLAQGMPPEQAAMAAVFVHGQAADQWPEQMPLTAGTLAQRLA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The AMP binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "LWGQEQVRRTYPKRAPDAHKGTAGKACVFAGSFTMPGAAVLAGKAAIRGGAGLTTIVTPKSALPLIAPSLTEATYVAASDEDGHFSGELPALGSFDAITVGPGIGRRKGSALLVDQLLEAENVPLLLDADALYHLSEDVSRLKRRGSPTILTPHPGEMAQLCGVKVQDVQTERLNLSRRFCQEYGVFLVLKGPHTVVTTPSGEQFVNHSGNAALAKGGTGDVLTGVITAFMMSHGSVQAALCNAVWLHGKVAEVLVEEKHSLTDVTASDVIDGLPSVLY",
    "response#2": "GVAAGSAQYPGAAHMVVGAARHCGIGMVRLHAEPGQREAADAVVVRFPDVVVTEAAPKKDGRATGWTVGPGLGTDESAARVLESALRSDLPVVVDADGLTLLADHDELRSLVRSRRAPSVLTPHVGEFARLGFELGLDRIAATKIAAVESGAVIVLKGSSTVISDESGRVFVDMMGSPALATAGTGDALSGIVGAAICRAGEKASVETVAAAVYLHGLSGRII",
    "response#3": "GVAAGSAQYPGAAHMVVGAARHCGIGMVRLHAEPGQREAADAVVVRFPDVVVTEAAPKKDGRATGWTVGPGLGTDESAARVLESALRSDLPVVVDADGLTLLADHDELRSLVRSRRAPSVLTPHVGEFARLGFELGLDRIAATKIAAVESGAVIVLKGSSTVISDESGRVFVDMMGSPALATAGTGDALSGIVGAAICRAGEKASVETVAAAVYLHGLSGRIL"
  },
  {
    "reference": "MMPVSGWRAVSSAPASSSAGRTANRIDFARSPRPTVGVEWEFALVDAETRDLSNEATAVIAEIGENPRVHKELLRNTVEVVSGVCGSAGEAIEDLRTTLGPARRIVHARGMELFCEGTHPFAQWSTQKLTDAPRYAELIKRTQWWGRQMLIWGVHVHVGVSSANKVMPIISALLNYYPHLLALSASSPWWGGEDTGYASNRAMMFQQLPTAGLPFQFQTWSEFEGFVYDQKKTGIIDHINEIRWDIRPSPHLGTIELRICDGVSNLRELGALVALMHCLVVDLDRRLDAGETLPTMPPWHVQENKWRAARYGLDAVIILDAESNERLVTDDLDDVLTRLEPVARSLSCADELAAVAEIPRVGASYQRQRRVAEEHDGDLRAVVDALIAELDI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must possess ATP binding to support cellular modified amino acid biosynthetic process under certain conditions.2. The protein have enhanced ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-   cysteine + H(+) + phosphate.3. For general function, the protein need meet that ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity. The designed protein sequence is ",
    "response#1": "AWLEKHPQALKGIQRGLERETLRVNADGSLATTGHPESLGSALTHKWITTDFAEALLEFITPVDGDIDHMLTLMRDVHRYTARQLGDERMWPLSMPCYIEQGQDIELAQYGTSNIGRLKTLYREGLKNRYGALMQTISGVHYNFSLPMAFWQAKCGETDKEAISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLQGKPTTLPFEKTDCGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLHEYVAGLKRAIKTPSEEYETIGLEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGEAPSDALERGGIEYIEVRSLDINPFSPIGVDEQQIRFLDLFMVWCVLADAPEMSSDELLCTRTNWNRPDVYNDALMPLLL",
    "response#2": "AWLEKHPQALKGIQRGLERETLRVNADGSLATTGHPESLGSALTHKWITTDFAEALLEFITPVDGDIDHMLTLMRDVHRYTARQLGDERMWPLSMPCYIEQGQDIELAQYGTSNIGRLKTLYREGLKNRYGALMQTISGVHYNFSLPMAFWQAKCGETDKEAISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLQGKPTTLPFEKTDCGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLHEYVAGLKRAIKTPSEEYETIGLEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGEAPSDALERGGIEYIEVRSLDINPFSPIGVDEQQIRFLDLFMVWCVLADAPEMSSDELLCTRTNWNRKSRHAWDPTPYARAWLEKHPQALKGIQRGLERETLRVNADGSLATTGHPESLGSALTHKWITTDFAEALLEFITPVDGDIDHMLTLMRDVHRYTARQLGDERMWPLSMPCYIEQGQDIELAQYGTSNIGRLKTLYREGLKNRYGALMQTISGVHYNFSLPMAFWQAKCGETDKEAISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLQGKPTTLPFEKTDCGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLHEYVAGLKRAIKTPSEEYETIGLEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGEAPSDALERGGIEYIEVRSLDINPFSPIGVDEQQIRFLDLFMVWCVLADAPEMSSDELLCTRTNWNRKSRH",
    "response#3": "AWLEKHPQALKGIQRGLERETLRVNADGSLATTGHPESLGSALTHKWITTDFAEALLEFITPVDGDIDHMLTLMRDVHRYTARQLGDERMWPLSMPCYIEQGQDIELAQYGTSNIGRLKTLYREGLKNRYGALMQTISGVHYNFSLPMAFWQAKCGETDKEAISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLQGKPTTLPFEKTDCGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLHEYVAGLKRAIKTPSEEYETIGLEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGEAPSDALERGGIEYIEVRSLDINPFSPIGVDEQQIRFLDLFMVWCVLADAPEMSSDELLCTRTNWNRRGQRLATHPEAPPAWLEKHPQALKGIQRGLERETLRVNADGSLATTGHPESLGSALTHKWITTDFAEALLEFITPVDGDIDHMLTLMRDVHRYTARQLGDERMWPLSMPCYIEQGQDIELAQYGTSNIGRLKTLYREGLKNRYGALMQTISGVHYNFSLPMAFWQAKCGETDKEAISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLQGKPTTLPFEKTDCGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLHEYVAGLKRAIKTPSEEYETIGLEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGEAPSDALERGGIEYIEVRSLDINPFSPIGVDEQQIRFLDLFMVWCVLADAPEMSSDELLCTRTNWNRGQRLAT"
  },
  {
    "reference": "MENVVIVSAVRTPIGSFNGALSSVSAVDLGAIVIQEAIKRANIESALVNEVIMGNVLQAGLGQNPARQAALKAGIEKEIPSLTINKVCGSGLKSVALGAQSIISGDADIVVVGGMENMSQAPYLLDSKVRQGVKMGNLTLRDTMIEDGLTCASNHYHMGITAENIAEQYGISRQAQDELALRSQTLASQAVQLGVFDKEIVPVMVKTRKGDIIVSRDEYPKADTTAEGLAKLKPAFKKEGTVTAGNASGINDGAAALILVSESKAHALGLKAIAKIRSYASGGVDPSVMGLGPVPATQKALKKAGINLDDIDLIEANEAFASQFLGVGKDLNLDMNKTNIHGGAIALGHPIGASGARILVTLLHNLIEKDKKLGLATLCIGGGQGISMIVERL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should aim to mimic or enhance the function of natural proteins involved in fatty acid beta-oxidation, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "LPVPSLEESLKKYLESVKPFANEEEYKNTEAIVWKFQNGIGEKLQQKLLQRAKGRRNWLEEWWLNVAYLDVRIPSQLNVNFGGPASHIEHYWPPKEGTQLERGSISLWHNLNYWQLLRKEKLAVEKVGNTPLDMNQFRMLFSTCKIPGITRDSIINYFRTESEGHSPSHLAVLCRGRVFVFDVMHEGYLMTAPEIQRQLTYIQKKCHSEPDGPGVAALTTEERTRWAKAREYLISLNPENLTILEKIQSSLLVFCLDDDSPHVTPEDYSQVSAKILNGDPTVRWGDKSYNLIAFSNGVFGSNCDHAPFDAMVLVKVCYYVDENILENEGRWKGSEKVRDIPVPEELVFTVDEKVLNDINQAKAQYFKQVSDLQLVVYAFTSFGKKLTKEKQLHPDTFIQLALQLAYYRLHGRPGCCYETAMTRLFYHGRTETVRPCTVEAVNWCQSMQNPSTSLLERKHMMLEAFAKHNKMMKDCSTGKGFDRHLLGLSLIAKEEGLPVPELFTDPLFSRSGGGGNFVLSTSLVGYLRVQGVMVPMVHNGYGFFYHIRDDRFVVSCSAWKSCPETDAEKLVQAMVTL",
    "response#2": "LPVPSLEESLKKYLESVKPFANEEEYKNTEAIVWKFQNGIGEKLQQKLLQRAKGRRNWLEEWWLNVAYLDVRIPSQLNVNFGGPASHIEHYWPPKEGTQLERGSISLWHNLNYWQLLRKEKLAVEKVGNTPLDMNQFRMLFSTCKIPGITRDSIINYFRTESEGHSPSHLAVLCRGRVFVFDVMHEGYLMTAPEIQRQLTYIQKKCHSEPDGPGVAALTTEERTRWAKAREYLISLNPENLTILEKIQSSLLVFCLDDDSPHVTPEDYSQVSAKILNGDPTVRWGDKSYNLIAFSNGVFGSNCDHAPFDAMVLVKVCYYVDENILENEGRWKGSEKVRDIPVPEELVFTVDEKVLNDINQAKAQYFKQVSDLQLVVYAFTSFGKKLTKEKQLHPDTFIQLALQLAYYRLHGRPGCCYETAMTRLFYHGRTETVRPCTVEAVNWCQSMQNPSTSLLERKHMMLEAFAKHNKMMKDCSTGKGFDRHLLGLSLIAKEEGLPVPELFTDPLFSRSGGGGNFVLSTSLVGYLRVQGVMVPMVHNGYGFFYHIRDDRFVVSCSAWKSCPETDAEKLVQWAEGL",
    "response#3": "LPVPSLEESLKKYLESVKPFANEEEYKNTEAIVWKFQNGIGEKLQQKLLQRAKGRRNWLEEWWLNVAYLDVRIPSQLNVNFGGPASHIEHYWPPKEGTQLERGSISLWHNLNYWQLLRKEKLAVEKVGNTPLDMNQFRMLFSTCKIPGITRDSIINYFRTESEGHSPSHLAVLCRGRVFVFDVMHEGYLMTAPEIQRQLTYIQKKCHSEPDGPGVAALTTEERTRWAKAREYLISLNPENLTILEKIQSSLLVFCLDDDSPHVTPEDYSQVSAKILNGDPTVRWGDKSYNLIAFSNGVFGSNCDHAPFDAMVLVKVCYYVDENILENEGRWKGSEKVRDIPVPEELVFTVDEKVLNDINQAKAQYFKQVSDLQLVVYAFTSFGKKLTKEKQLHPDTFIQLALQLAYYRLHGRPGCCYETAMTRLFYHGRTETVRPCTVEAVNWCQSMQNPSTSLLERKHMMLEAFAKHNKMMKDCSTGKGFDRHLLGLSLIAKEEGLPVPELFTDPLFSRSGGGGNFVLSTSLVGYLRVQGVMVPMVHNGYGFFYHIRDDRFVVSCSAWKSCPETDAEKLVQEITQL"
  },
  {
    "reference": "MTTTVIILAAGKGTRMRSNLPKVLQPLAGRPLLGHVIETAKKLQAANIITIYGHGGDLVRNAFNQEKIEWVEQTEQLGTGHAVKVTLPVLAKEGVSLILSGDVPCVQQSTLQKLVEASTETGIGLVTLTLEDSTGYGRIVRKNGKIQAIVEHKDATEEQRQIKEINTGIYCVSNAKLHEWLPKLSNENAQGEYYLTDIVAMAIADGMEIASIQPEFAFEVEGVNDRIQLAALEREFQQHQAKTLMQQGVHLIDPSRFDLRGQLTVGKDVRIDINVIIEGECSFGDNVEIGAGCIIKNTQIAANTKVQPYSVFDSAVVGGNVQIGPFARLRPGAQLSDDVHIGNFVEVKNSSIGLGSKANHFAYLGDAEIGSGSNIGAGTITCNYDGANKHKTIIGDEVFIGTNNSLVAPVKIGNGATTGAGSTITRDVAEHALAVERSQQFTKENYQRPQKVKK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that is capable of catalyzing reactions within the Nucleotide-sugar biosynthesis pathway with high efficiency and specificity.2. The protein must bind to Mg(2+) in order to perform its enzymatic function.3. The designed protein should be involved in peptidoglycan biosynthetic process, regulation of cell shape, UDP-N-acetylglucosamine biosynthetic process, cell wall organization by localizing to cytoplasm and exhibiting magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity, glucosamine-1-phosphate N-acetyltransferase activity.4. The designed protein should be able to compete with endogenous proteins or inhibitors for acetyl-CoA, Mg(2+) binding.5. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc) The designed protein sequence is ",
    "response#1": "VVILAAGQGKRMRSDLPKVLHRLGGRPLLAHVLDTARELGAGRICVVYGHGGEAVREALDADDLVWVRQEPQLGTGHAVLQTLTFLDADAPTLVLYGDVPLIGAATLSRLVGLEAADPGRYGRLVTAGDVLARIVEYKDATPEQREIGLCNSGVMCADAAALFDLIEAVGNDNASGEYYLTDIVGIARARGLGAGVVRCPEAETLGVNTRAELAAAEAAFQARARAAAMAAAGATVVDPATTWIGVNVTIGSDVIIHPNTQLWGATSIADGAEVGPDTTLTNMQVGANAHVIRVHGFDSVIGRGANIGPYTYIRPGTVVGEEGKLGGFVEAKNAQIGRGTKVPHLTYIGDATVGEYSNIGASSVFVNYDGVNKHHTTIGSHVRTGSDTMFIAPVTVGDGAYSGAGTVIKDDVPAGALVVSGGKQVNRTGLGRADLARAEAAFQARARAEALENGVTLIAPETVFFAHDTVIGRDALIEPNVIFGPGVTVESGAHIRAFCHLEGAHVSRGAVIGPFARLRPGAELSEDVRVGNFVEVKNALVAEGAKINHLSYIGDATVGAEANIGAGTVTCNYDGVFKHHTEIGARAFIGSDTMLVAPVRIGEGAMTAGGSVITSDVPDGALALGRARQVNKPGWGALFRKAKAQRDQGS",
    "response#2": "VVVLAAGGGTRMKSKKATLLHEVSGKTMLSYAVSAAAALDPERLVVVVGHLRDQVAEHLETLSTTVSTAIQDQQLGTGHAVAAGLETLGELTGEVVVTYGDVPMLTGTTLRKLVAIHRAHNNGVTLLTAHVEEPTGYGRIIREGDKVVGIVEQADATEEQRAVKEINSGIYVFESELLRAGLEQLDTDNATGEHNLTDVVRFANERGDRVGSFVTDDVWQTEGVNDRAQLAELMTAGVTLIAPETVFLSHDTAIEADVTIEPFVVFGPGVRVATGTTIRAHSHLEGADIAADCDIGPFARLRPGTSLARGVKIGNFVETKKAVMAEGAKANHLSYLGDATIGAHANIGAGTITCNYDGYFKYQTVIGAGAFIGSNSALIAPVKIGRDAIVAAGSAVSRDVADGELRMVRAEGVTLLDPDRIDVRGELDCGRDVTIDVSCVFEGRVVLGDGVEIGAHCVLKNAVVGAGTRIAPFSHIEDAEAGSGCVIGPYARLRPGTKLGADVHIGNFVEVKNSTVADRSKANHLAYVGDATIGRNVNVGAGTITCNYDGASKHRTVIEDDVFIGSDTQLVAPVTVGRGATLGAGTTLTADAPPDKLTISRARQVNKPGWAAKKRASQAKTE",
    "response#3": "ALVLAAGKGTRMHSSRPKVLQTLLGEPMLRYVYDAIKPLFGQAAITVVGHGSGLVEQAFPEMAGQFVLQAQQLGTGHALQVAWDAVKADGTDYCLVINGDTPLITAEGLGRLAGQAGGLGLLTVELADPTGYGRIVRGADGRVAAIVEHKDASEAQRAIRETYTGIMAAPTARLKRWVARLDNSNAQGEYYLTDIVAMAVAEGVPVVAHAAPDDVETVQPGVTIGPFAHLRPNSHLGKNVHIGNFVEVKGVTMHDPATTTIEDSVEIAPDVILEAGVILRGATRVETGAHIGAYSVVCDSFVGEDVHVKPHSVLEGARVEAGANVGPFARLRPGAVLEEHAHVGNFVEVKNARLGRGVKAGHLAYLGDADIGEDTNIGAGTITANYDGLNKHKTVIGRGVFVGSNTTLIAPATLGDAAFVAGGSAVTGDVPEGALIVARGAPARAITRPWKTPKPEK"
  },
  {
    "reference": "MTIAPTTLLLFAATALAELVGCYLPYLWLRKGGSMWLLLPTALSLAVFVWLLSLHPEASGRVYAAYGGVYIASALLWLWWVDGVTPTRWDLLGAACCLLGMAVIMFSPRSA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have a specific function within the plasma membrane.2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "STTPPWWAIPPAWSLGAEIQAYFLLPILLTYKMLGLSVFWISYIIYSLANLNIIHSDYFGYRLIPGVIFMFLSGAYLQKIVSGKASRLEMLSLIIIYIISLFWLVFFIIIKGKYGAYTRETLLGLLVGIPLVYTLLKIRRKFYFNDLFGKLSYGIFLSHFLSFWILEFVNLTQNIISMIFLSLIISASVSYLL",
    "response#2": "STTPPWWAIPPAWSLGAEIQAYFLLPILLTYKMLGLSVFWISYIIYSLANLNIIHSDYFGYRLIPGVIFMFLSGAYLQKIVSGKASRLEMLSLIIIYIISLFWLVFFIIIKGKYGAYTRETLLGLLVGIPLVYTLLKIRRKFYFNDLFGKLSYGIFLSHFLSFWILEFVNLTQNIISMIFLSLIISASVSYLL",
    "response#3": "STTPPWWAIPPAWSLGAEIQAYFLLPILLTYKMLGLSVFWISYIIYSLANLNIIHSDYFGYRLIPGVIFMFLSGAYLQKIVSGKASRLEMLSLIIIYIISLFWLVFFIIIKGKYGAYTRETLLGLLVGIPLVYTLLKIRRKFYFNDLFGKLSYGIFLSHFLSFWILEFVNLTQNIISMIFLSLIISASVSYLL"
  },
  {
    "reference": "MKITVYMTGGIAVYKAVEVVRHLQKAGHKLRVVMTKNAERFVSSNTLAALTKQPVLDDLWTKEHEATIPHIELARWTQLAIVVPASANFLAKMANGIADDAASTTILATAAPKAVVPAMNDQMWSNPATQRNLSLLKKDGIAIMEPVVGLLAEGYQAKGRMPEPDQIFSWLEEKYLTANKRLQGKKIIVSAGGTIESIDPVRYISNHSSGKMGIEIAKAARDAGADVTLVYGNIQAALPDGINTIAVKSAQDMYEAIATNFNDADVLIMAAAVGDWRAKTVAKNKLKKQADQAELQLTFVKNIDILKTLAHAKRADQLVIGFAAETTELLENAQRKLEQKGADYIVANDVSQNVFGSDEDQVTILAQNKQPEKWPKMTKKQVAEKLLNLIRKRF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein should be able to manipulate the activity of enzymes in the coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway, either by activating or inhibiting their function.2. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.3. The designed protein should have phosphopantothenoylcysteine decarboxylase activity, metal ion binding, phosphopantothenate--cysteine ligase activity to facilitate pantothenate catabolic process.4. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.. The designed protein sequence is ",
    "response#1": "MRIVLGVSAGIAAYKAVYLARLLKEDGHQVDVIPTESSLEFVGNATWEAVSGRPVSTSVFEGVEEVRHVRLGQEADLIVVAPATADFLARVRAGMASDLLTTTLLATEAPILLVPAMHTEMWRNAATQDNVAVLRDRGIDVMEPAVGRLTGADSGPGRMPEPDQIRALISLAKPLLLRKGRRILLVIGGGIAAYKSLELIRRLRERGATVRAVMTPAACQFITPLSVGALTADRVFTDLFDREDEHDVGHIRLAREADLIVAAPATADLLAKMANGLANDLASTILLAAKVPVLVAPAMNPAMWQHPATRRNVAQLAGDGVAFVGPNSGAMAEAGEAGEGRMAEPLEIVAAIERRLSVASGPLAGKSVLITSGPTHEPIDPVRYIANRSSGKQGHAIAAAAARAGARVTLVSGPVTIPDPAGVRVLRVETAREMLQAVEAALPADVAIMAAAVADWRVASGSGQKIKKDGSGKPPALSLAENPDILATVGTLAEGRPCLVVGFAAETQNLIDNARAKLQRKGADFIVANDVSPETGVMGGDANTVRLVSRDGVEDWPTLSKEDVAERLVAVIAAKLADSGL",
    "response#2": "MRIVLGVSAGIAAYKAVYLARLLKEDGHQVDVIPTESSLEFVGNATWEAVSGRPVSTSVFEGVEEVRHVRLGQEADLIVVAPATADFLARVRAGMASDLLTTTLLATEAPILLVPAMHTEMWRNAATQDNVAVLRDRGIDVMEPAVGRLTGADSGPGRMPEPDQIRALAEEVRRLLAPRDLLAGKSVLITSGPTHEPIDPVRYIANRSSGKQGHAIAAAAARAGARVTLVSGPVTIPDPAGVRVLRVETAREMLQAVEAALPADVAIMAAAVADWRVASGSGQKIKKDGSGKPPALSLAENPDILATVGTLAEGRPCLVVGFAAETQNLIDNARAKLQRKGADFIVANDVSPETGVMGGDANTVRLVSADRVEEWPRMTKDEVARRLAARIAEALG",
    "response#3": "MRIVLGVSAGIAAYKAVYLARLLKEDGHQVDVIPTESSLEFVGNATWEAVSGRPVSTSVFEGVEEVRHVRLGQEADLIVVAPATADFLARVRAGMASDLLTTTLLATEAPILLVPAMHTEMWRNAATQDNVAVLRDRGIDVMEPAVGRLTGADSGPGRMPEPDQIRALVGFAAGTPSRLAGRRVLITAGGTREPLDPVRYLGNRSSGKQGLALADAARSAGAQVTLIAGSMEQQPGPELACIEVVSVGTTEQMHAAVMSRAEQADALIMSAAVADFRPADYAHTKIKKSDNGASPIITLERNPDILAETVARRNHRGTGPKVIVGFAAETGDEKSDPLELAQLKLQRKGCELLVLNRVGENLVFGLDSTEIHLTPGSPTLSGLHVVVSAGGTREYLDPVRFLGNRSSGRQGYALAEAAVARGARVTVVAANVALPDPAGAEIIRVVSTEDLRQQMHLAAADADAVVMAAAPADFRPAEFVEAKIKKTADGVAPDIHLVENPDILLELVGARTSKRPVIVGFAAETGDARASVIEHARAKLARKGCDLLVVNDVSAGKVFGQDESEAVVLGRDGSEHEIPRAEKSALAHAVWDAVVPLL"
  },
  {
    "reference": "MPGKKSPAGEFAARKLREKRKKFRWKDERYKRRMLKLDEKADPLEGAPQARGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKQVTAFCPGDGAIDYIDEHDEVVIEGIGGPKGRAKGDIPGVRYKVVKVNDVALSELLKGKIEKPMR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be involved in translation by localizing to small ribosomal subunit and exhibiting structural constituent of ribosome, rRNA binding.2. The protein must exhibit the following characteristics: With S4 and S5 plays an important role in translational accuracy; Located at the interface of the 30S and 50S subunits.. The designed protein sequence is ",
    "response#1": "PRKGNVQKREVLPDPIYNSKLVTRLINHMMMDGKRGTSTKIIYGAFDIIKNETGNDPVETFEEAMKNIMPVLEVKARRVGGSNYQVPIEVRPDRRTTLGLRWLVQYSRLRGEHTMVERLAREIMDAANNTGAAVKKREDTHRMADANR",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTC",
    "response#3": "MRKHSAPIRKVLADPVFNSVLVTKLINAIMLDGKKSLAQDILYSAFNLVKEKTQKDPMEVFQAAIDNVTPQLEVRTRRIGGTNYQVPTEVSARRKQTLALRWLVQYARLRNEKTMDVRLANEIIDASNKTGGAIKKREDTHKMAEANF"
  },
  {
    "reference": "MAVPGSFPLLVEGSWGPDPPKNLNTKLQMYFQSPKRSGGGECEVRQDPRSPSRFLVFFYPEDVRQKVLERKNHELVWQGKGTFKLTVQLPATPDEIDHVFEEELLTKESKTKEDVKEPDVSEELDTKLPLDGGLDKMEDIPEECENISSLVAFENLKANVTDIMLILLVENISGLSNDDFQVEIIRDFDVAVVTFQKHIDTIRFVDDCTKHHSIKQLQLSPRLLEVTNTIRVENLPPGADDYSLKLFFENPYNGGGRVANVEYFPEESSALIEFFDRKVLDTIMATKLDFNKMPLSVFPYYASLGTALYGKEKPLIKLPAPFEESLDLPLWKFLQKKNHLIEEINDEMRRCHCELTWSQLSGKVTIRPAATLVNEGRPRIKTWQADTSTTLSSIRSKYKVNPIKVDPTMWDTIKNDVKDDRILIEFDTLKEMVILAGKSEDVQSIEVQVRELIESTTQKIKREEQSLKEKMIISPGRYFLLCHSSLLDHLLTECPEIEICYDRVTQHLCLKGPSADVYKAKCEIQEKVYTMAQKNIQVSPEIFQFLQQVNWKEFSKCLFIAQKILALYELEGTTVLLTSCSSEALLEAEKQMLSALNYKRIEVENKEVLHGKKWKGLTHNLLKKQNSSPNTVIINELTSETTAEVIITGCVKEVNETYKLLFNFVEQNMKIERLVEVKPSLVIDYLKTEKKLFWPKIKKVNVQVSFNPENKQKGILLTGSKTEVLKAVDIVKQVWDSVCVKSVHTDKPGAKQFFQDKARFYQSEIKRL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Mono-ADP- ribosylates STAT6; enhancing STAT6-dependent transcription; In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation; However, the role of ADP-ribosylation in the prevention of STAT1 phosphorylation has been called into question and it has been suggested that the inhibition of phosphorylation may be the result of sumoylation of STAT1 'Lys-703'; Has been shown to catalyze the mono-ADP-ribosylation of STAT1 at 'Glu-657' and 'Glu-705', thus decreasing STAT1 phosphorylation which negatively regulates pro-inflammatory cytokine production in macrophages in response to IFNG stimulation.2. The designed protein should be involved in negative regulation of type II interferon-mediated signaling pathway, negative regulation of tyrosine phosphorylation of STAT protein by localizing to membrane and exhibiting enzyme binding, NAD+-protein ADP-ribosyltransferase activity.3. The designed protein have Macro, PARP catalytic domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "QMLLVKEGVQNAKTDVVVNSVPLDLVLSRGPLSKSLLEKAGPELQEELDTVGQGVAVSMGTVLKTSSWNLDCRYVLHVVAPEWRNGSTSSLKIMEDIIRECMEITESLSLKSIAFPAIGTGNLGFPKNIFAELDSSGNTAAHYAAAYGFLDCLKLLASIDDNILSEPNDWQLYPLSVAYLKGHYGIVTWLLEGPHKDKANINAKSWEKGSLVSPGGLQMLLVKEGVQNAKTDVVVNSVPLDLVLSRGPLSKSLLEKAGPELQEELDTVGQGVAVSMGTVLKTSSWNLDCRYVLHVVAPEWRNGSTSSLKIMEDIIRECMEITESLSLKSIAFPAIGTGNLGFPKNIFAELIISEVFKFSSKNQLKTLQEVHFLLHPSDHENIQAFSDEFARRLKANEDVHQKIPAHWSDMKQQNFCVVELLPSDPEYNTVASKFNQTCSHFRIEKIERIQNPDLWNSYQAKKKTMDAKNGQTMNEKQLFHGTDAGSVPHVNRNGFNRSYAGKNAVAYGKGTYFAVNANYSANDTYSRPDANGRKHVYYVRVLTGIYTHGNHSLIVPPSKNPQNPTDLYDTVTDNVHHPSLFVAFYDYQAYPEYLITFRK",
    "response#2": "MHYAACAPGTAPMEFLLKNGGSVTMLTKQTETPLHVAARAGRAVNCTFLMKEMLDLEKGDDGESTIRADRSIINARTRSGNSALHLAVLRNNLDVVDALLVNAANENLLHGSGLAGSLVKTGGFEIQEESKRIIANVGKISVGGIAITGAGRLPCHLIIHAVGPRWTVTNSQTAIELLKFAIRNILDYVTKYDLRIKTVAIPALSSGIFQFPLDLCTSLEDNTPLHYALTNGNLMLFNLMLDKVAEQNSYEFKVISQYLQSTHAPTHSDYTMTLLDLFEVEKDGEKEAFREDLHNRMLLWHGSRMSNWVGILSHGLRIAPPEAPITGYMFGKGIYFADMSSKSANYCFASRLKNTGLLLLSEVALGQCNELLEANPKAEGLLQGKHSTKGLGKMAPSSAHFVTLNGSTVPLGPASDTGILNPDGYTLNYNEYIVYNPNQVRMRYLLKVQE",
    "response#3": "VNAANENLLHGSGLAGSLVKTGGFEIQEESKRIIANVGKISVGGIAITGAGRLPCHLIIHAVGPRWTVTNSQTAIELLKFAIRNILDYVTKYDLRIKTVAIPALSSGIFQFPLDLCTSSYEFKVISQYLQSTHAPTHSDYTMTLLDLFEVEKDGEKEAFREDLHNRMLLWHGSRMSNWVGILSHGLRIAPPEAPITGYMFGKGIYFADMSSKSANYCFASRLKNTGLLLLSEVALGQCNELLEANPKAEGLLQGKHSTKGLGKMAPSSAHFVTLNGSTVPLGPASDTGILNPDGYTLNYNEYIVYNPNQVRMRYLLKVQP"
  },
  {
    "reference": "MTALPPWLEPLPDASEQRALDEWAIKELGIPGLELMERAGSGLAKLVSDRAPQGPIAIVCGKGNNGGDGYVAARVLREQGRSVRVLTLVDPGELRGDAQANFERLPGPPPDPFAPERLKGAAGIVDAILGTGFSGTPREPAASAIEAINAAGDTEGRQAVEVIACDVPSGVNGSTGEVEGAAVRATATATFHAGKPGLWIAPGKDHAGEVRTVDIGIPQGGPSGPSIGLINADVVDAIPRRGRESTKFAAGSVLVCGGSTGLTGAPSMASESAMRAGAGYVTALVPGSLNAIFEVRLLEVMTVPLPDEGGSLTTEALEVVIERCQRADALVLGPGFGRQEAALELARRIAREAPVPLLLDADGLNAHAGSVKTLAEREAAAVLTPHAGELARLLETNSGAVAARRLESVRGAASTAASVVLLKGDDTLVASPDGRVAVSRGGASALATAGTGDVLSGVIGAFLAKRMDAFEAACAGVFVHARCGQLAARDKGPEGVIASDVIERLPAALRDPGA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must have high specificity towards metal ion binding.2. The designed protein should be able to compete with endogenous proteins or inhibitors for AMP, K(+) binding.3. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. The designed protein sequence is ",
    "response#1": "MYEDQEIRFPERRADGNKGTFGKVLLIAGSMNMCGAAILSGKAAFRSGCGMVKIMTVEENRVILQESFPEAMLLTYTEDGFDSEELSRSMDWCDCIGIGPGLSQGETALRLVEFILANAAVPVVADADALNIIAKRPEILSSCQTEVVVTPHIGEFGRLTGLETEEIKTHFYDVAADFARRFSVVCVLKDARTIIVNKDGEAFINCTGNSGMATAGSGDVLTGIICSLIAQGLGPFAASSLGCALHGRAGENGSAFRSPTELMAGDIIEGIVS",
    "response#2": "LIVGGSFSMPGAVSMTASACIYSGAGLLTTALPASILSIVSNSVPETMFLPLPEKNGYIAGEVMGTDFTFGHYDAVAAGPGLGRERGGKQLIRKLLAEVKTPLLFDADALYYLPELKQEMKQREEPVIVTPHAGEMARLADVSIQEVNKHRFSISKEFARAYNCYVVLKGPYTIVTSPEGEQSINPTGNEALARGGTGDILTGIALGFMLQHRDVFSALCNAVYIHGLTADLALQKGFTATSMASSDLIHQLA",
    "response#3": "MYEDQEIRFPERRADGNKGTFGKVLLIAGSMNMCGAAILSGKAAFRSGCGMVKIMTVEENRVILQESFPEAMLLTYTEDGFDSEELSRSMDWCDCIGIGPGLSQGETALRLVEFILANAAVPVVADADALNIIAKRPEILSSCQTEVVVTPHIGEFGRLTGLETEEIKTHFYDVAADFARRFSVVCVLKDARTIIVNKDGEAFINCTGNSGMATAGSGDVLTGIICSLIAQGLGPFAASSLGCALHGRAGENGSAFRSPTELMAGDIIEGIVE"
  },
  {
    "reference": "MRVRGKQRSWPQWWIWGILGFWIILMCRVMGEEWVTVYYGVPVWREAKATLFCASDAKAYEKEVHNVWATYACVPTDPNPQEILLGNVTENFNMWENDMVDQMHQDIISLWDQGLKPCVKLTPLCVTLNCTDKVVNATRNDTAVNATSSGNATYDSNMQGEIKNCSFNITTEIIDKKQKAYALFYRTDIVPLKKNSSEYILINCNSSTITQACPKVSFDPIPIHYCAPAGYAILRCNNKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIIRSKNLADNTKTIIVHLNESVAINCTRPNNNTRKSIRIGPGQAFYATNDIIGDIRQAHCNISEEKWNTTLERVRKKLEEHFPNKTINFTQHAGGDLEIVTHSFNCRGEFFYCNTTQLFNNSNPDNGTITLPCRIKQIINMWQEVGRAMYAPPIEGIITCNSNITGLLLTRDGGNTTNNTEIFRPGGGDIKDNWRSELYKYKVVEIKPLGVAPTTSRRRVVEREKRAAGIGAVLLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQFLGLWGCSGKLICTTNVPWNSSWSNKSQADIWDNMTWMQWDREINNYTDTIYRLLEESQNQQEKNEKDLLALDNWKNLWNWFSITNWLWYIKIFIMIVGGLIGLRIIFSVLSIVNRVRQGYSPLSFQTLTPIPREEPDRLGRIEEGGGEQGRDRSIRLVNGFLAIAWDDLRSLCLFSYHQLRDF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain.2. The designed protein must have at least one Retroviral envelope protein GP41-like domain. The designed protein sequence is ",
    "response#1": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGRSGKIICTTAVPWNSTWSNKSFEEIWNNMTWTEWEREISNYTSQIYDILTESQNQQDRNEKDLLELDKWVSLWNWFDITKWLWYIKIFIMIVGGLIGLRIIFAVLSIVF",
    "response#2": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGRSGKIICTTAVPWNSTWSNKSFEEIWNNMTWTEWEREISNYTSQIYDILTESQNQQDRNEKDLLELDKWVSLWNWFDITKWLWYIKIFIMIVGGLIGLRIIFAVLSIVF",
    "response#3": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGRSGKIICTTAVPWNSTWSNKSFEEIWNNMTWTEWEREISNYTSQIYDILTESQNQQDRNEKDLLELDKWVSLWNWFDITKWLWYIKIFIMIVGGLIGLRIIFAVLSIVF"
  },
  {
    "reference": "MRLHHLLLTLLFLVLSAGSGFTQGISNPLSCRLNRGICVPIRCPGNLRQIGTCFTPSVKCCRWR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Has antibacterial activity..2. The ideal protein would be one that plays a critical role in defense response to bacterium through chemoattractant activity, CCR6 chemokine receptor binding within extracellular space.3. The protein must contain a signal peptide for proper functionality. The designed protein sequence is ",
    "response#1": "DNILVVFILVKYKGLRQAENMSFLNLALSNLGFLLTLPFWAYAASHGEGFDDPLCKILLLLYSIGLYSEAFFNVLLTVQRYKEFFHVRRRFSACRTVAGSIFISVLVWVTATLVTLPELVSYKPQMQSQKYKCFFTGLHFLPADETFWKHFLTLKMNILGFLLPLFAFVYCYVRMRKTLQFRERNYGLFKLVFTIMAVFLLMWGPYNIVLFLSAFNEHFSLHGCGSSYNLNKSVQITRIIAATHCCVNPLLYACLGDHFRKTFKALLCR",
    "response#2": "DNILVVFILVKYKGLRQAENMSFLNLALSNLGFLLTLPFWAYAASHGEGFDDPLCKILLLLYSIGLYSEAFFNVLLTVQRYKEFFHVRRRFSACRTVAGSIFISVLVWVTATLVTLPELVSYKPQMQSQKYKCFFTGLHFLPADETFWKHFLTLKMNILGFLLPLFAFVYCYVRMRKTLQFRERNYGLFKLVFTIMAVFLLMWGPYNIVLFLSAFNEHFSLHGCGSSYNLNKSVQITRIIAATHCCVNPLLYALLGKDFRAAARAL",
    "response#3": "DNILVVFILVKYKGLRQAENMSFLNLALSNLGFLLTLPFWAYAASHGEGFDDPLCKILLLLYSIGLYSEAFFNVLLTVQRYKEFFHVRRRFSACRTVAGSIFISVLVWVTATLVTLPELVSYKPQMQSQKYKCFFTGLHFLPADETFWKHFLTLKMNILGFLLPLFAFVYCYVRMRKTLQFRERNYGLFKLVFTIMAVFLLMWGPYNIVLFLSAFNEHFSLHGCGSSYNLNKSVQITRIIAATHCCVNPLLYALLGRDFRKQLAKILCCGRH"
  },
  {
    "reference": "MKIAVVGATGMVGQVMLKVLEERNFPITELIPVASERSIGSKVKYKGTEYEVVSMQTAIDKKPEIAIFSAGGGTSLDWAPKFAEAGIFVIDNSSAWRMDPTKKLVVPEINADVLEKEDKIIANPNCSTIQLVMVLHPLNKKYDIKRVVVSTYQSVTGTGKAAVDQLNAEIEGKDADKVYPYQIFKNALPHCDVFADDDYTKEEIKLMKEPKKILGDDSFNLTATAVRVPVQGGHSESVNIEFENEFDLDEVKKILSETPGVTVLDDVKNNQYPMPLMAEGKDDVFVGRIRRDISQPKTLNCWIVADNLRKGAATNAVQIAEYLLDKKLV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..2. The designed protein must possess protein dimerization activity, NADP binding, NAD binding, aspartate-semialdehyde dehydrogenase activity to support threonine biosynthetic process, diaminopimelate biosynthetic process, lysine biosynthetic process via diaminopimelate, 'de novo' L-methionine biosynthetic process, isoleucine biosynthetic process under certain conditions.3. The Proton acceptor, Acyl-thioester intermediate of the protein must be engineered to enable effective catalysis of L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.4. The NADP(+), phosphate binding site should be located in a region of the protein that is accessible to the ligand.5. The designed protein should contain one or more Semialdehyde dehydrogenase NAD-binding domains that are essential for its function.6. The protein design should consider the endogenous proteins and enzymes present in the Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5 pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MQTVAIIGATGAVGREMISILEQRAFPMKALRLFASPRSVGTRIPFGGTSIAVEALEHGMPEGIDLVLFSAGKGISKEWAPKFREAGALVIDNSSAFRADAECPLVVPEVNPQALSSLPKGIIANPNCTTMAAMPVLKPLHDIAKVKRVVVATYQSVSGAGKEAMDELWSQTRGIFVNDPPLAEQFTKPIAFNVIPHIDAFMDDGSTKEEWKMAVETRKILDPDIQVVATCVRVPVMIGHGEAVHVEFERPITEKQARDALREAPGITVMDHREDGGYATPIEIAGEDSVFVSRIRRDPTVPHGLSFWCVGDNLRKGAALNAVQVAELIVRVAIVGATGAVGQEFCAVLAARKFPAASCRMLASARSAGKRVDWAGSSQVVEELAPAAFDGIDVAFFSAGASVSREFAPLAAKRGALVVDNSSAFRMDPNVPLVVPEVNPQDARSRPRGIIANPNCTTMAAMPVTPLHRAIGVERMVVATYQASSGAGASAMAELESQAHAFAAGTAMDTTIFGRQYLFNVFSHNSAVGDDGYNEEERKLLTETRRMWESDTVRISATCVRVPTLRAHAEAIHLEFARATSEAEVRALLAKAPGVRIVDDRSANRFPEPLDAAGGDDVLVGRIRMDQSIAGNRGVALFVCGDQIRKGAALNAVQVAELVVAEMAG",
    "response#2": "MTKIAVVGATGNVGREMLSILAERAFPADEVVALASRRSQGSEVSYGDKTLKTKTLDTYDFSDVDICLMSAGGEVSKEWSPKIAAQGAVVIDNSSAWRYDADVPLVVPEVNAEALRDNVAIVGATGEVGRTFLKVLEERNFPVKELRLFASSKSEGRTMTFKGHEYKMEALEKQTSFKGIDIALFSAGSSVSKEWAPKFAQDGVVVIDNSSAWRMDDDVPLVVPEVNKEDIKKPPKGIIANPNCTTMAAMPVLKPLHDKFKVKRVVVSTYQSVTGAGKRGIDQLFAEIKGDKILNPKFPHQIAFNCIPHIDNFYEDGYSKEEHKIMKETIKIIGDNSIKITATCVRVPVIGGHSEAVNIEFEKDFEIEEIRDVLNNSPGIVLYDDPYNNAYPMPLISHEKDEVFVGRIRRDYSSENALNLWIVADNLRKGAALNAVQIAELVAAELAE",
    "response#3": "MAKIAVVGATGNVGREMLSILAERAFPADEVVALASRRSQGSEVSYGDKTLKTKTLDTYDFSDVDICLMSAGGEVSKEWSPKIAAQGAVVIDNSSAWRYDADVPLVVPEVNAEALRLYPLHKVNPIKRIIVDTYQAVSGMGSPAAIEELVTQAKLVLSGQGTVPHVFPHQIAFNALPEIDVFLDNGYTKEEWKMVEETRKIMHADSIAISATCVRVPVFTSHSEAVHVEFSQVIXPEEARRILSQAPGVKVLDDPVISLYPQPWAAAGTDEVFVGRVRQDASKSCGLAFWIVADNLRKGIIANPNCTTMAAMPVLGALHAAYGLRELVVSSYQAVSGAGRAGVETLRRQIALVAGTELGTSPGDVRRAVGEDTGPFPEPVALNVVPWAGTPREDGWSSEEMKVRDESRKILGLPKLPVAVTCVRVPVVTTHSLTVHARFEDTVTVDGAREIIATAPGVVLFDNPEAGEYPTPADVVGTDPTWVGRVRRALDDPTALELFVCGDNLRKGAALNAVQIAELIVERRAA"
  },
  {
    "reference": "MEVILLERVAKLGQMGEVVKVKHGFARNFLLPRGKALRATTDNRAKYEHMKADLEARNIEAKAEAIKVAEKIDGRNVVVLRQASETGQLFGSVTVRDIISQFTADGVHIERSQVLLDHPVKTIGKHSISIAVHPEVEVSVSVTVARSALEAERINRGEDISTRQEDQDAAAEALAAAGEFFDPEAHNDGEQEEEAGDK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that contributes to translation by functioning in ribosome, ribonucleoprotein complex with rRNA binding, structural constituent of ribosome.2. For general function, the protein need meet that Binds to the 23S rRNA. The designed protein sequence is ",
    "response#1": "MI",
    "response#2": "MLEAKYKIDRRMGQNIWGRPKSPVNRREYGPGQHGQRRKGKLSDFGVQLRAKQKLKGYYANISERQFHAIYVEATRLKGDSGENLIGLLG",
    "response#3": "MRYTGPMWKISRRLGISLSGTGKELQKRPYPPGQHGPGQRRKLSEYGLQLQEKQKLRHMYGVNERQFRKTFEEAGKMPGKHGENFMILLS"
  },
  {
    "reference": "MVETGNKNERLTSSVEACNRWRDHGWRDKVATWAGRLLVLAAFVSLAGVFFHRSVILRVVMDVLGWVNIPSEPSLFAACMALVLAGGIRRRFRPAHTVLAVVMGLSVLDSLARLVFAHQVTQMINRLHVRGVYRDWAQFQTSPGVSAAILALSVTSFVVVLLARPAFRARFSSGSKAAALISLIGGLFLSFVVAFLLTLSFPRDLDTLGQKFVWSVRSTFGVRTPPASPGFGGHHGYEWTFVVASTVSTLVLIFSLLVLWRSGRGSGLIDADEELRIRRLLLEHGEDDSLGYFATRRDKAVVFAPDGRAAVTFRNEGSVSVASADPIGRAESWDDAIAVWLGQCREHGLYPAVLSASTRGARAYVEAGLKAWSLGDEAIIDTDTFSLRGRSMRPVRQAVTRVERCGYTTQVRRHDELGDDELAELSRLADDWRGNETERGFSMALNRLGDRADGRCVVITAHDTRGAVRGFLSFVPWGSRGLSLDLMRRDRQAENGLNEFMVAHLAEAAPALGIRRISLNFAVFRNVFADADEVGAGPITRVTDKALSIASKFWQLETLYRSNDKYHPEWVPRFLCYDAELTVPRAGFAMGVAEGFVPLIGPRLLVGPKPADHQPPRPEAVFVERVQALEADALTPRMPEPRRSEQQRVRREKLARMEAGGVEGYPVAVPRTARIEDVVTAHGRLPADHRTGERVSVTGRVRALRDFGGVSFVVLDEDGARLQAMATRDVTPAEQYDHWRHWIDLGDIVSVTGEIVTSKHAELSVHVS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.2. The designed protein should have a binding site that is specific to the target Mg(2+) ligand.3. The protein must exhibit the following characteristics: LPG synthesis contributes to the resistance to cationic antimicrobial peptides (CAMPs) and likely protects M.tuberculosis against the CAMPs produced by competiting microorganisms (bacteriocins); In fact, the modification of anionic phosphatidylglycerol with positively charged L-lysine results in repulsion of the peptides.; Catalyzes the production of L-lysyl-tRNA(Lys)transfer and the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge.4. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.5. The designed protein must have at least one Aminoacyl-transfer RNA synthetases class-II family profile domain.6. We need a protein that can perform ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-   tRNA(Lys) with high efficiency. The designed protein sequence is ",
    "response#1": "MKAAVPRVLSTVLAVLAVYCLLIALVAPLRHALVPVTETVSVGLFTLEANLAYAVFIALLAGAVARRKRVAYWVFVAVVVITLLADAVTVPVYVFIPELRPEFPAAFFIATTVNLVLMTAVLVIMVAAYGEFYAKVQHAAFRKALTTVIALLIVSFAIGMGLVTLFPGTLPKDAHLTWVLEKTLGGAISFDFHTSGHPPQWVGFLIGLISATALLAGAAVLLRQVPRAFATLLWVIATVCALAALSSAVRSDIQPVRTFIDTLLLPAPANLAYAVFLGTLASAVLRRKRLAYRVQVVYFSLSLLVGLVAGGLLLFVGAERLTDDAGQRIFNLIETVGVWVGVGFAAVALTMLLAARREFYARVRPGSTWRALAVFLGLAAAAVGLGYLLLLVEPGSLVTWSDRLGYAAEKVFGGAVTLDLTRRGQAPAWVNLLLGAFGAAALVVGLFTLRQVPRAFATLLWVIATVCALAALSSAVRSDIQPVRTFIDTLLLPAPANLAYAVFLGTLASAVLRRKRLAYRVQVVYFSLSLLVGLVAGGLLLFVGAERLTDDAGQRIFNLIETVGVWVGVGFAAVALTMLLAARREFYARVRPGSTWRALAVFLGLAAAAVGLGYLLLLVEPGSLVTWSDRLGYAAEKVFGGAVTLDLTRRGQAPAWVNLLLGAFGAAALVVGLFTRRAWVRRRLRSRERVTSWSGRVVTVAGVWLVVSFPLRHRTWVQWIDAAFELLNVPTARNLFVIVALLLLGSALRRRLRSALIVVIAYQLAAGLLQLPVVAIAAANWSDLDATDLDLDRTEVVLTAVSAAVGVVLGVALLRVRRAFPARLAPGSRRAALAVLAGGLIASWATTVWLTQLFPHTLSGAREHLTWATRSAFGVTAAGDQAGLNGHLGHHWVAVLGGTLSASALLGAVA",
    "response#2": "REQVARWIGRVYLFAAIWSLISVPLRHNIVVEIVADLLGLVNVPSEPSLFIVCLLFVLAGAVRRRLRAGFTMVVILMGISVLVSLAVMLSAIFRQEWLQDTQLRGFERDYYSFITSVPVTAVFFAIGLAVLVVVARSRSAFPARLAEGSRLAAVLVLVAGLATSFAITFALAATAPKELVGTREIGRFALRSTFGIASDSTSPGMHGHVGYAWIYGLAGIMSTIALLLASLAAVPRVLSTVLAVLAVYCLLIALVAPLRHALVPVTETVSVGLFTLEANLAYAVFIALLAGAVARRKRVAYWVFVAVVVITLLADAVTVPVYVFIPELRPEFPAAFFIATTVNLVLMTAVLVIMVAAYGEFYAKVQHAAFRKALTTVIALLIVSFAIGMGLVTLFPGTLPKDAHLTWVLEKTLGGAISFDFHTSGHPPQWVGFLIGLISATALLAGAAVLTTSLDVQASGIGLRFHWVPAAAGWIIGVIASLSLLSSVSTVLRHLIRIPREFINDYIFNFPDTSFAWAFVLALLAAALAARKRIAWWILVFYMAAAGLWNIADALSGGETEAVDIGEIVGLAFHLASIGILLVAYKEFWARVRRGALLKATATLVAGLAVGTLVGWSLLEFFPGSLAREDRFWYALNRVGAFAGAGAESFTGHPHGLVNALLGLFGALALMVAAVVLAPTIFFWYTRLSGILSLLAWVSYGLILEIADIWALRWIGYLGWFPSVPIGLVWILLSMGIRRRKKAAWRILVLIFCLPTVLGVTAVVTTLLNPDKTIHIGLVVTAAVYLAVLALLVAARGEFTTLPDRANRRLATHVFTNLLVVTCGIGTFLVTVTDRDNSGAFWTHPVYAILQTVLGPRVTGKPIDVLVPLWVDAILWVLGTGLLIATFWAM",
    "response#3": "RAGGLVATVVQLAALFSVVLLLAGGRHGHLLNGIDMAFGVLSVPTSSSLVMVLLLVVLGAALRRRKKAALYVLVLFQVAGLVTTLVLQGLLLWAPDLLTLNARQMRHIPTQVGALAIADVISILLIVFLLSLRPAFPARLAPGAWWNGLTVLFVGFLGVIVLGWALTEVFPGHLGDTWERFAWVVNHATGENLQLRRIGVGEGPAWIDVVLDLGATFVSTAALYTLLNGLWGRTPLAGVAPTIFFWYTRLSGILSLLAWVSYGLILEIADIWALRWIGYLGWFPSVPIGLVWILLSMGIRRRKKAAWRILVLIFCLPTVLGVTAVVTTLLNPDKTIHIGLVVTAAVYLAVLALLVAARGEFTTLPDRANRRLATHVFTNLLVVTCGIGTFLVTVTDRDNSGAFWTHPVYAILQTVLGPRVTGKPIDVLVPLWVDAILWVLGTGLLIATFWAMRYSWVPAAAGWTVGIIATLSLIASVSPLVRSIIKIPREFVNDYIFNFPDTSFAWAFVLAMLAAALAARKRIAWAILVLYMVASIGWNLGDLATGEETWMQESGEIIGLAFHVAAVVFLLLARKEFWAKVRRGALLKAALTLVAGMAIGTLIGWGLLELFPGTLEREYRLGYAANRVAAFAGVGSGAFDGQSPHVFIDALLGLFGALALMVAAIVLGRRGR"
  },
  {
    "reference": "MTRIKRGYIARRRRTKMRLFASSFRGAHSRLTRTITQQKIRALFSAHRDRDKQKINFRRLWIARINAVIRKRGVSYSYSKLIHDLYKRQLLLNRKILAQIAISNRNCLYMISNEIIKETERKEYTGII",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that It is not involved in the protein synthesizing functions of that subunit .2. The protein should be optimized for stability and expression in plastid, ribonucleoprotein complex, ribosome where it will participate in translation. The designed protein sequence is ",
    "response#1": "MGPIGICITLRGDSMYAFLDRLINLALPRIRDFQGVSSKSFDGHGNYNLGLKEQLMFPEIDYDQIDKIRGMDICIVTNAKTDSEGFYLLEVLGMPV",
    "response#2": "FELILQTVPTEKRVAVLKAIRNTTSLDLKGAKDSIADLPKKLLDNLSLEEAEKAKTLLEEAGATVL",
    "response#3": "EKNGNTLPISKIPTGSYICNIEARPGDGGKFVRASGVQAIIIDKTVDRVGVKMPSGKMKWFSSRCLATVGVVAGGGRGEKPFVKAGKKFHKVKSQATYWPRVRGVAMNVIDHPFGGGGHQHTGRPKTVPF"
  },
  {
    "reference": "MLFSRSPFRLCRISAVGQTRQLPFSRPRFLSASVARYAIDMETVNTSERLARLRQLMQEHKVDVYIVPSEDSHQSEYIAPCDGRREFISGFSGSAGTAIVSMTKAALSTDGRYFNQASKQLDSNWLLLKRGVENVPTWQEWTTEQAEGGKVVGVDPSLITAPGARSLAETLRKNGSSLVGVQQNLVDLVWGEDRPAPPREKVRVHPDKFAGKSFQEKITDLRKELENKKTAGFVISMLDEIAWLFNLRGSDIPYNPVFFAYAIITPTTADLYIDEEKLTPEVTSHLGQDVVIKPYDSIFADATALSEARKQDAGEAAAKFLLSNKASWALSLSLGGEEHVEETRSPIADAKAVKNEAELAGMRACHIRDGAALIEYFAWLENELVSKKTSLDEVDAADKLEQIRSKHDLFAGLSFDTISSTGPNGAVIHYKPEKGSCAIIDPEAIYLCDSGAQYLDGTTDVTRTFHFGQPTELEKKAFTLVLKGMIAIDSAVFPKGTSGFALDVLARQFLWKEGLDYLHGTGHGIGSYLNVHEGPIGIGTRVQYTEVPIAPGNVISDEPGFYEDGKFGIRIEICLADVIMAREVQTTHKFGDKPWLGFEHVTMAPIGRNLIEPSLLSESELKWVNDYHAEIWEKTHHFFENDEFTRSWLQRETQPISK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides.2. The protein can be expressed in cellular anatomical entity and perform metal ion binding without triggering undesirable cellular responses.3. The protein must be engineered to have a high affinity for Mn(2+) in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "NMARQLQDTPSDQLYPEEFVKRFEKAATGLGVKITVLKQADLIKKKMGLLLGVNKGSEREARLLVISYNNNKKSSETLALVGKGITYDSGGMNIKTGDYMRGMKYDMSGAAIVCSTVLALAKNKVKTNVVAVAALTENLPGPHAQRPDDIQTAYNGKTVEIDNTDAEGRLVLADAISYAAKDLKATQIIDVATLTGLMSYILSTTYTGIFSTCDMAWDAFKKAACCAGEPVWRLPMHPDYLKPLESKLADLQNSTSVKGAGSSRAACFLAEFREGVPLIHCDIASTASIQDLGQGVLVRTI",
    "response#2": "LAREMVAAPANEVTPLTFTEIATELAQTYGLELEVLGQTECEALGMGAFLGVAKASELPPQFIHLTYRPANPVKKLAIIGKSLTFDSGGLNIKGAGSGIETMKMDMGGGGATLGAAKAIAQLKPNVEIHFICAATENMISGTAMHPGDILTASNGKTIEVNNTDAEGRLTLADALVFAEKLGVEAIVDLATLTGACIVALGDDIGGLWSPNQELADELKVAADKAGEKFWQMPMESKYFEGLKSPIADMKNTGPRSGGSITAALFLQQFIKETPWAHLDIAGPVWTDKQNGVHNAGATGYPVRTLIR",
    "response#3": "NLTRTLVNEPGNRLTPTLLGEQAKKMCAEQGLRCQVYSSEKLHELKMGSFWSVTQGSDEPPALIVMEYTPEGAAEGPVLGLVGKGITFDSGGLSLKPADSMEKMKYDMAGAAAMIGAMRAIALLKPRIKVISVICSAENMPSGKAQKPGDVQISMIGKSIEVLNTDAEGRLVLADGLAYAKQLGATHLIDAATLTGAVMVALGGVNAGVFCNDEEAWQHFEAALGQSGEKFWRLPLDEEYREMLRSPIADIKNVGGRYGGASTAAMFLKEFVGDTPWVHLDIAGTAWMDEAKPWMSSGPSGIAMY"
  },
  {
    "reference": "MISKILCLSLLVAAVVADQVDVKDCANNEIKKVMVDGCHGSDPCIIHRGKPFTLEALFDANQNTKTAKIEIKASLDGLEIDVPGIDTNACHFMKCPLVKGQQYDIKYTWNVPKIAPKSENVVVTVKLIGDNGVLACAIATHGKIRD",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be designed to localize specifically to extracellular region in order to perform its role in sterol transport more effectively. The designed protein sequence is ",
    "response#1": "IVQQLEKAEESIYMQAYGMSDSLITTALINAQMRGVKVKILLDRSNLKQKFSKLYELQQAKIDVGIDTVPGIAHNKVIIIDKKKVITGSFNFTVAADKRNAENVILIEDQQLAESYLQNWFG",
    "response#2": "IVQQLEKAEESIYMQAYGMSDSLITTALINAQMRGVKVKILLDRSNLKQKFSKLYELQQAKIDVGIDTVPGIAHNKVIIIDKKKVITGSFNFTVAADKRNAENVILIEDQQLAESYLQNWFG",
    "response#3": "RFRAFAPKLRPLCLELEKRAIGPYLSYLYDTQNIYFNNRRSILSLIMFEKLQSLSKMTDISSMIRSSSLFMIQFYENEYQIYSNFFSPPDLNNNNNNNNNNNNNDNINNSTNINNSNNTNNNNQDIINNCPAFSNILDEYSQQLYDTIRPIYIHIHSFETLCNLAHLIRNELIDDLVQKSMKYSNGFKMTIERMLQDIQERLIFIIQTYIRDEIRSYHPNSDDLDYPNKLKIYVTAESTAVDGDGNGSGNSSPTLSYKSIYSTWYPTLEKSLTCLSKLYLVLETRIFEGLAQEVVEACTFTLIQASRLLLIQQKNDPYIILDSQLFLIKNLLTLREQIAPFDINFVIIEKIVDFR"
  },
  {
    "reference": "MSLKGKHIVLGITGSIAAYKSAILTRLLVKKGAEVQIVITAAGKEFITPVTLSALSGRPVISEFFATGDGTWHSHVDLGLWADLMLIAPATASSIGKMVSGIADNMLITTYLSMKAPVFVAPAMDLDMFKHVTTQRNIQQLKSDGVMIIDPAVGELASHLEGKGRMEEPENIVKTIEDYFMTQTVIEQEKTILEQDLLKKKILITAGPTYEKIDPVRFIGNYSSGKMGFAIAEECAKRGADVTLITGPTALNITHPNIKRIDVESANEMHNASTNIFPQMDVAILSAAVADYRPTQRVDTKIKRNNKEDITIKLTPNPDIAATLGKIKKENQITIGFALETNNEEENAINKIEKKNFDFIVLNSLNDTGAGFSVNTNKVTIISKEGTKTTFNLKSKLEVAKDIINTTLFNKTIKADINTPNKSG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should contain one or more DNA/pantothenate metabolism flavoprotein C-terminal domains that are essential for its function.2. The designed protein must be optimized for FMN binding.3. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.4. The protein should have a low Km value for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.5. The protein should be optimized for activity in CoA from (R)- pantothenate: step 3/5 pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "KRILLIIAGGIAAYKTLDLIRRLRERGAAVTPVMTAAAEGFVTPLSVSALAGEAVHRDLFDLTREAEMGHIQLSRAADLVVVAPATANLMAKMAGGLADDLASTLLLATDKTVLLAPAMNVRMWTHPATRRNLATLQSDGIRFVGPTEGDMACGEYGPGRMAEVPEILAAIEAALAAGAAGRDGLRVLVTAGGTREPIDSVRYVGNRSSGRMGFALADEAARRGARVTCLAANVGLDRNPVVEYVDVETAAQLAEATGARFDDCDVLLMAAAVADYRPAHALDEKIKKGAVQRLELELERTEDVLAGLAGRRRPDQVLVGFAAEHGQGGLERARGKLADKRLDAVVLNDVSRGDIGFDAGENEVTILAADGSETEVPRGAKTALAHALWDEVARRLA",
    "response#2": "RVLLHVSGSIAAYKAGEIVTLLRKRGAEVRVAMTHAATRFVSAMTFQSLSGHAVAADPWEPGEGGVAAHGMAHLGLGGWAEVQVVAPASADLIARLALGLADDAVTATALASTAPLLLAPAMETRMWEHPATRANLATLRARGAAVVGPLAGRLASGDEGMGRMAEPAEVVEACAATLRHAGGPRDGLRVLVTAGGTREPIDSVRYVGNRSSGRMGFALADEAARRGARVTCLAANVGLDRNPVVEYVDVETAAQLAEATGARFDDCDVLLMAAAVADYRPAHALDEKIKKGAVQRLELELERTEDVLAGLAGRRRPDQVLVGFAAEHGQGGLERARGKLADKRLDAVVLNDVSRGDIGFDAGENEVTILGADGSEVEVARGSKHDVADAVWDAVVRLLP",
    "response#3": "RLLLCVSGGIAAYKSLELVRRLCGAGAQVQVAMTASAQQFVTPLSFQALSGQSTRTTLWDGNAEQAMGHIELARWAERVLIAPASADVIARLAHGLADDLVTTLCLATTAPLMICPAMNHKMWLHPATQANVATLCSRGVQVIGPEDGPLAEKVSGLGRMSEPEVIVEPGGRGRMAEPEALRILLIVGGGIAAYKSCEIVRTLRRRGHAVRCVLTEGGSHFVTPMTLAALSEDQVFTTLWDLKDEAEMGHIQLSRQADLVVIAPATADLLARMAGGLANDLATTLLLATDKPVLAAPAMNVRMWQHPATRRNVEQLRADGVTVMEPDEGAMACGEFGPGRLPEPDVIVARIEQVELLLAGP"
  },
  {
    "reference": "MNRKTVFRNLAIVLLIILAIWGWSAMRDSNREYKTVDTYVALTQLNTKSNVEFVQIDDREQRLRISLKEPAVVNNKGEKADKITTQYPAASAREVFDRIDATGAGYQTNVSQDSIFGQLLVFLLPMLLLLGLFIFVMSRMQGGGKGGVMGFGKSRAKQLTKDMPKTTFADVAGADEAVEELYEIKDFLQNPSRYQALGANIPKGVLLYGPPGTGKTLLARAVAGEAGVPFFTISGSDFVEMFVGVGASRVRDLFEQAKANSPCIIFVDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFGERSGVILIAATNRPDILDPALLRPGRFDRQIPVGNPDMAGRKAILNVHAKGKPIAPDADLDGLAKRTPGMSGADLENVLNEAALLTARENKTVISGEALEEAVDRVIGGPRRKSRVISEHERKVVAYHEGGHTLAAWAMPDLDPIYKVTILARGRTGGHALAVPEQDKDVMTRSEMIARLVMAMGGRAAEELVFSEPTTGASSDIDQATKIARAMVTEYGMSAKLGAVRYGQEQGDPFLGRSMGTQSDYSADVAREIDDEVRRLIEAAHTEAWAILNEYRDTLDILATELLERETLTRKDLEKIFSDVEKRPRITEFNEFGTRIPSDKPPVKTPGELAIERGEPWPPEPEPKAEPVSAQTPNYAPGYAPQPNYPGAGSGANYPGQQQHPGQPYPPQPYPPQPYPTQPYPGAGNGASGPNYPGAGNGASGPNYPGAGSGASGPNYPGQQQHPGQPYPPQPYPTQPY",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins The designed protein sequence is ",
    "response#1": "LLVGPPGTGKTLLARAIAGEAGVPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLGNGNDEREQTLNQLLVEMDGFEANEGIIILAATNRPDVLDPALLRPGRFDRQVIVV",
    "response#2": "IPKGALLVGPPGTGKTLLARAIAGEAGVPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLGNGNDEREQTLNQLLVEMDGFEANEGIIILAATNRPDVLDPALLRPGRFDRQVIVPRPLHFEFIDPADD",
    "response#3": "IPKGALLVGPPGTGKTLLARAIAGEAGVPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLGNGNDEREQTLNQLLVEMDGFEANEGIIILAATNRPDVLDPALLRPGRFDRQVIVPRPLSFL"
  },
  {
    "reference": "MPIITLPDGSKRAFEDAVTVMQVASDIGAGLAKATIAGRVNGQLKEASDLITEDAELQLITLKDEDGLHIMRHSCAHLLGHALKQLYPAAKMAIGPVVENGFYYDIDMEEKITPEDLKQIEKRMKELAKTKYEVIKEMTPRAEALATFKERQEDYKVELIEDMPDETEFGLYHHQEYIDMCRGPHVPNMGFIKAFKLTHVAGAYWRGNSDNKMLQRIYGVAFADKQELKDYLRMMEEAEKRDHRKLGKSLDLFHVDELAPGMAFWHPKGSTLYRVVENYMRQQLVENDYQEIRTPLIMDRTLWEKSGHWDKFKDNMFTTETENRDYAVKPMNCPGHIQVYNHNLSSYRDLPIRLAEFGLVHRNEPSGTLHGLMRVRSFTQDDAHIFCTPEQIKEEVQACIDLVFNTYADFGFDNIQVKFSTRPEQRVGEDDVWDLAEEALEQTLKDANLEYALQPGEGAFYGPKIEFQLKDCIGRVWQCGTIQLDFSMTQEERLNAVYIGADNEKHHPVMIHRAILGSLERFVGILVEHYEGKFPTWLAPTQLVIASIADVHNEYVNDFAKKLKKHGFRVESDLRNEKVGFKIREHTLQRVPYILVVGDQEMENGTVNVRARGGENLGSFSFEELIDHLSEDVSRLGRIVES",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have a binding site that is specific to the target Zn(2+) ligand.2. The protein can be targeted to cytoplasm and perform metal ion binding, tRNA binding, threonine-tRNA ligase activity.3. The protein must exhibit the following characteristics: Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr).4. The protein must bind to Zn(2+) in order to perform its enzymatic function.5. The protein should have a fast turnover rate for ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-   threonyl-tRNA(Thr). The designed protein sequence is ",
    "response#1": "MQLLLIHSDYIEYETKKQTPVAEKIEESLKSGRLEEALTAFTAVESVDEANPEEAIKKAVSEIEKVAAQVKTNRIMLYPYAHLSSDLSSPKVAVQVLKGMEAALSSRYEVKRAPFGWYKAFTVSCKGHPLSELSRSLGMLFREVHYAIEADKKHLFLRFAACFGQFLMGHDMTFSYRSLPLRMFELTRYSFRREQRGELVGLRRLRAFTMPDMHTFCGDMSSAMEEFRKQYEASISVLRDAGLDLKDYEVAIRFTKDFYENNKTFIEGLVDIVDKPVLIEMWDERFFYFVLKFEFNFVDALDKASALSTVQIDVENAERYDIGYVDAEGNRQRPIILHCSPSGAIERLMYALLEEFGYAIEADKKHLFLRFAACFGQFLMGHDMTFSYRSLPLRMFELTRYSFRREQRGELVGLRRLRAFTMPDMHTFCGDMSSAMEEFRKQYEASISVLRDAGLDLKDYEVAIRFTKDFYENNKTFIEGLVDIVDKPVLIEMWDERFFYFVLKFEFNFVDALDKASALSTVQIDVENAERYDIGYVDAEGNRQRPIILHCSPSGAIERLMYALLEEFGYAIEADKKHLFLRFAACFGQFLMGHDMTFSYRSLPLRMFELTRYSFRREQRGELVGLRRLRAFTMPDMHTFCGDMSSAMEEFRKQYEASISVLRDAGLDLKDYEVAIRFTKDFYENNKTFIEGLVDIVDKPVLIEMWDERFFYFVLKFEFNFVDALDKASALSTVQIDVENAERYDIGYVDAEGNRQRPIILHCSPSGAIERLMYALLE",
    "response#2": "MQLLLIHSDYIEYETKKQTPVAEKIEESLKSCRLEEALTAFMAVESVDEANPEETIEKTVFEIEKVATQVKTDRIMLYPYAHLSSDLSSPKVAVKVFKGIETALSGKYEVKRAPFGWYKAFSISCKGHPLSELSRSSVLDESTPEQGFVLKPMNCPFHIQIYKSELRSYRDLPLRLAEFGTVYRYEQSGELGGLTRVRGFTVDDSHLFVTPEQLDAEFLNVVDLILTVFRCLRLNKFKARLSFRDPKSDKYIGSDEAWSKAEAAIQRAVEQLGMEHFLGIGEAAFYGPKLDFIFEDALGREWQLGTVQVDYNLPERFDLEYVAEDNTRRRPVMIHRAPFGSLERLIGILIEEPTQVAILNITDKQADYCKKIGEILNDKGFRVRLDLRNEKINYKIREHTLNRVPFLAVVGDKEVETQCLAIRTRQGEDLGVMSISAFEELLKR",
    "response#3": "MDNEQLVLRPMNCPHHMMIYKSKIHSYRELPIRIAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKDEFKRVVNLILEVYKDFGLDEYSFRLSYRDPHDKEKYYDDDEMWEKAQNMLREAMDELGLEYYEAEGEAAFYGPKLDVQVRTALGKDETLSTVQLDFLLPERFDLTYIGEDGKPHRPVVIHRGVVSTMERFVAFLIEEGYAAAKKAELETDDKTLALKPMNCPCHVQIFKQGIKSYRDLPLRMSEFGLCHRNEASGALHGLMRVRSLVQDDAHIFCAAEQITDETVSFCKLLTEVYKDFGFTDIKVKFSDRPEIRAGSNEVWDKAENALKEAVEQAGFNYTLNPGEGAFYGPKLEFVLTDAIGRQWQCGTLQMDFVLPERLDASYVAASGEKKRPVMLHRAILGSLERFIGILIEEGYAAAKKAEKEGYSIESDKRHMFLRFAACFGQFLMNHDMTISYKNLPLRMIEMTRYSFRKEQRGELVGLRRLRAFTMPDMHTLCEDMDQAVNQFKEQYDLCISVLENVGIHINDYEVAIRFTRDFYEANKDLVVNMAKTVNKPVLVEMWDTRFFYFVLKFEFNFVDALAKASALSTVQIDVENAERYDISYVNADGKLERPTVLHCSPSGAIERCIYALLEGYAAAKKAEKQVAILNITDKQADYCKKIGEILNDKGFRVRLDLRNEKINYKIREHTLNRVPFLAVVGDKEVETQCLAIRTRQGEDLGVMSISAFEELLQQVAIATITSDLNDYALEVQKALIDNGIRTDFNISPDKINYKIREFSNQKIPMIAVIGKQEQENKQVAIRRLGTTDQEVLSVEQLIAVVK"
  },
  {
    "reference": "MAHFFKENSSCYFCFRYLENPVYLNCGYICCFQCLDSLEKSPEGDGVLCPNCSVVSLKEDIRHAKQLRNLVTTVKDLEPQLNFILTMDPSMKIFQVNMILDVDTAQNNLIISEDLRSVYHTSQTQDRKKCAERFDPSPCVLGSSRFTSGCHYWEVVVGTSKEWDIGICKESINRQEVVYLSEKKGFWTVGVRNEEVYAASTDPLTVLLVNPRLHRVGIFLDLLEKSVSFWDLGDGSHIYTFLEIPDTEPFRPFFSPANTYPDEEQVISICPVMNPSIFGLPVNPV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that can perform ubiquitin-protein transferase activity, metal ion binding in chromatin, nucleus, nucleoplasm, cytoplasm, female germ cell nucleus to support positive regulation of DNA-templated transcription.2. The protein contains novel B30.2/SPRY domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MWIQVRTMDGKVAHTVDSLSRLTKVEELRKKIQELFHVEPGLQRLFYRGKQMEDGHTLFDYDVRLNDTIQLLVR",
    "response#2": "HFGPIPGIPVGTMWRFRVQVSESGVHRPHVAGIHGRSNHGAYSLVLAGGYEDDVDHGNSFTYTGSGGRDLSGNKRTAEQSCDQKLTNTNRALALNCFAPINDLKGAEAKDWRSGKPVRVVRNVKGRKHSKYAPIEGNRYDGIYKVVRYWPEKGKSGFLVWRFLLRRDP",
    "response#3": "HQKQALAWMIARENSKELPPFWEQRNDLYYNTITNFSVKERPENVHGGILADDMGLGKTLTAIAVILTNFDDGRPLLSKRGKKNHPGKEYKDETIKRRGSNMDKKEDGHSESSTCGEEPSISGTPEKSSCTLSQLSSVCPKRRKISVQYIESSDSEEIETSELPQKMKGKLKNVQLNTKSRVKGSSKVKEDSKFALTFFASATQRKMLKKGMSMMECSEACDTGERTRATLIICPLSVLSNWIDQFGQHVKSEVHLNFYVYYGPDRIRDSAWLSKQDIILTTYNILTHDYGTKDDSPLHSIKWLRVILDEGHAIRNPNAQQTKAVLELEAERRWVLTGTPIQNSLKDLWSLLSFLKLKPFIDREWWYRIIQRPVTTGDEGGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSEEERKIYQSVKNEGKAAIGRYFTEGTVLAHYADVLGLLLRLRQICCHTCHVCGIKQDPDKQLLCDECDMAFHTYCLNPPLTTIPDDEDWYCPDACKVCKDDPKKNCRVCNCHVCGIKQDPDKQLLCDECDMAFHTYCLNPPLTTIPDDEDWYCPDA"
  },
  {
    "reference": "MAESQAKSPGGCGSHESGGDQSPRSLHVREQDRFLPIANISRIMKRGLPANGKIAKDAKEIVQECVSEFISFVTSEASDKCQREKRKTINGDDLLWAMATLGFEDYMEPLKVYLMRYREMEGDTKGSAKGGDPNAKKDGQSSQNGQFSQLAHQGPYGNSQAQQHMMVPMPGTD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must be able to DNA-binding transcription factor activity, RNA polymerase II-specific, DNA-binding transcription factor activity. The designed protein sequence is ",
    "response#1": "FGVQETAIISQEVHNFIMDQGWSEYQFCNQIWAGKCPKTIRMFYSNLYKKLSHRDAKSIYHHVRRAYI",
    "response#2": "FLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFM",
    "response#3": "FGVQETAIISQEVHNFIMDQGWSEYQFCNQIWAGKCPKTIRMFYSNLYKKLSHRDAKSIYHHVRRAYS"
  },
  {
    "reference": "MSLVTGSHAAVTEPEALASFLLGHEPRRIHLIGVAGSGMSGIAALLLALGHHVTGSDKVETLEIDRLRRKGLEFETPHGTKLVELAELVIYSSAIHPGNPALDTALSLGKKLARRAEILAAVMSGKEGIVVCGMHGKTTTSAMAAHVLRAGGLKPSHYVGAEIPILGTNARWVSEGKYFVAEGDESDGTLVHYHPSHALLLNIEPEHLDHYEDIEAINRVFGQLLSQTSGNVYYWADDEGASRVCSSHERSIPVGTSKQCRYRIEGLEENGFVTRFAVFKGEELLGSVLLGISGAHNASNAMLVIALACDLGIQFETIATALESFRGAKRRFELKYHDPSNRIFDDYGHHPTEIQATLATARKGLTGKGRLVVLFQPHRFTRTAALREQFGKAFRDADVVFVTPVYSAGENPIEGADAGSIVMAAKADGHPSIATVGSLKKGANAVAACLKEGDLLLTLGAGNIHEAASYLAEELQLGARLREVVGSGSLRFSEPLSKHTTMRVGGPARFWVEPETEEGFSELVRFCHDENIPFMVMGRGSNLIVRDGGFPGVVAHLARGCFSECRVLGNKITVGVGVKLKQLAATARNAGLTGFEWMDGIPGNLGGALRMNAGAMGVQTFEQVESIRFSDSDGNIISRTPAEMEVRYRDIPLLKDHFALSATLIGVPASTETIDDLLATSLRHRKESQPVAASSGCVFKNPSGISAGRLIDELGLKNHSLGGARVSDIHANFIVNDGNATASEILGLIQQVRDAALEKRGVLLETEV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The designed protein should have ATP binding to facilitate regulation of cell shape. The designed protein sequence is ",
    "response#1": "LQDAYKSIEESLLQAAVYNGRRIKIRLIHSEKITPSNVGEMLAGLDGVIIAPGFGQRGIEGKFTAIRWCRETDIPTLGICLGMQCMVIEFARNVLGLEGANSTEMDPGTPYNVIDLMEEQKSITNLGGTMRLGAYRCSLLPGSLAAKAYGKTDVTERHRHRFEFNSDYRARFEAAGMKCVGENPETHLVEVVEIPSRRWMIGTQYHPEYSSTVLSPSPLFISFVTSMVE",
    "response#2": "LQDAYKSIEESLLQAAVYNGRRIKIRLIHSEKITPSNVGEMLAGLDGVIIAPGFGQRGIEGKFTAIRWCRETDIPTLGICLGMQCMVIEFARNVLGLEGANSTEMDPGTPYNVIDLMEEQKSITNLGGTMRLGAYRCSLLPGSLAAKAYGKTDVTERHRHRFEFNSDYRARFEAAGMKCVGENPETHLVEVVEIPSRRWMIGTQYHPEYSSTVLSPSPLFISFVLS",
    "response#3": "LQDAYKSIEESLLQAAVYNGRRIKIRLIHSEKITPSNVGEMLAGLDGVIIAPGFGQRGIEGKFTAIRWCRETDIPTLGICLGMQCMVIEFARNVLGLEGANSTEMDPGTPYNVIDLMEEQKSITNLGGTMRLGAYRCSLLPGSLAAKAYGKTDVTERHRHRFEFNSDYRARFEAAGMKCVGENPETHLVEVVEIPSRRWMIGTQYHPEYSSTVLSPSPLFISFVAA"
  },
  {
    "reference": "MPAKKKTTRRNTKKELQKKAATRKMIAFFVGLLLILFALARLGIVGILLYNIVRLFIGSLAIILLLLVAAIMILSVFRKQFLKENKRIIPAIILTFIGLMFVFQIRLHQGLNETFHLIWSDLTAGRVIHFVGSGLIGAIITEPAKALFSVIGVYIIAAVLWLVAIYLMIPGLFPKMREDLHQRLAKWKEKRAEKVEAKKAVKALKKLEEEKEIPEPQTILPEAENSLFTSAPVEIPINIPEAPFEENENPVLEENPVDDEPVNFMNTNNYNGNYKLPTIDLLAEVPVKNQSGERENVRKNIGILEETFKSFGIGANVESAVVGPSITKYEIKLATGTKVSRVVNLSDDLALALAAKDIRIEAPIPGKSLVGVEIPNAEVAMVGFREMWEAGKTNPSKLLEIPLGKSLDGGIRTFDLTRMPHLLVAGSTGSGKSVAVNGIITSILMKALPSQVKFLMVDPKMVELSVYNDIPHLLIPVVTNPRKASRALQKVVDQMEERYELFSRYGVRNIAGYNEKVQRYNAESDEKMLELPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIHMILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDTNGAEKLLGRGDMLFKPIDENHPVRLQGAFLSDDDVEAVVTFIKDQSEAQYDESFDPGEVDENQVGTGASNTGSGDPLFEEARNMVIIAQKASTAQLQRALKVGFNRASDLMNELEAQGIVGPAKGTTPRKVLVSPDGEFIGGVEE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Essential cell division protein that coordinates cell division and chromosome segregation.2. A protein that can perform DNA binding in plasma membrane to support cell division.3. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.4. The presence of FtsK domains is critical for the interaction of the designed protein with its intended targets.5. The designed protein should contain Cytoplasmic topological domains. The designed protein sequence is ",
    "response#1": "LPQYHLLSKRNIRRPESLLEELKKKAAILQQTLASFGIDASIGNICSGPTLAAFEVLPNTGVKVQKIKALENDIALNLQASSIRIIAPIPGKAAVGIEIPG",
    "response#2": "RWGDKAVLNKQGEVLIPDDIDEFKNLPLITGPEGQEKKLLEIMKGVYIVLKDKSMQLAEFHVNDRRAWRIKLASGLEMQLGRKAPLENMQRFLKTMDLLGEEQVAMMASVDTS",
    "response#3": "RLKECCLILGVLTSAFLSIALLTFSPADPSWSQTSWGGDISNAGGQFGAWVADTLFFTFGLLAYLLPVLLVIVTWVFFRTRDEDEHIDLMLWGTRLLGLAILILTSCGLADINFDDIWYFSSGGVLGDVLNSLALPTLNLLGTTLVLLFAWGAGFTLLTGISWLSIVEWIL"
  },
  {
    "reference": "MALGVPISVYLLFNAMTALTEEAAVTVTPPITAQQGNWTVNKTEADNIEGPIALKFSHLCLEDHNSYCINGACAFHHELEKAICRCFTGYTGERCEHLTLTSYAVDSYEKYIAIGIGVGLLLSGFLVIFYCYIRKRCLKLKSPYNVCSGERRPL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The Extracellular topological domain of the protein should be designed to be flexible or rigid.2. The designed protein have EGF-like domains with specific sequence motifs that are crucial for its activity.3. A protein that can perform transmembrane receptor protein tyrosine kinase activator activity, epidermal growth factor receptor binding in plasma membrane, extracellular region to support positive regulation of epithelial cell proliferation, angiogenesis, positive regulation of cell population proliferation, epidermal growth factor receptor signaling pathway.4. The engineered protein is expected to adopt a strand-strand-turn-strand-turn-based conformation.5. The protein should contain a Helical membrane-spanning region.6. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "DKDELRVEFSTVQPSGLLFYARNSGGPFADYVALELVGGRLRFSIRYGRSSHSTENLHETLLGKNLNDAKSHSVEILHDKDVTTIYLDKTSDQEKAEHSFKTKYTKLDIDVAMYVGGAFDFKALLSVKSNALFMGCIFQAEFKKC",
    "response#2": "PQIYEKAVSSFPDPALYPLGSRQILTCTAYGIPQPTIKWFWHPCNHNHSEARCDFCSNNEESFILDADSNMGNRIESITQRMAIIEGKNKMASTLVVADSRISGIYICIASNKVGTVGRNISFYIQ",
    "response#3": "GLVSYNAPAGQQFVLPGGSIIYLNDSVYDGAVGYSMTEGLGQLTDGVSGLDDFTQTHEYHVWPGYDYVGWRNESATNGYIEIMFEFDRIRNFTTMKVHCNNMFAKGVKIFKEVQCYFRSEASEWEPNAISFPLVLDDVNPSARFVTVPLHHRMASAIKCQYHFADTWMMFSEITFQL"
  },
  {
    "reference": "MQAYQRDFIRFAIDRGVLRFGEFTLKSGRTSPYFFNAGLFNTGSALAQLGRFYAAAVVESGIAFDVLFGPAYKGIPLASATAVALAEHHDRDLPWCFNRKEAKAHGEGGSLVGSPLAGNVLIIDDVITAGTAIREVMQIIKDQDATAAGVLIALNRQERGNGELSAIQEVERDFGIPVVSIVSLNQVLEFLADDPQLKQHLPAVEAYRAQFGI",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must possess orotate phosphoribosyltransferase activity to support 'de novo' UMP biosynthetic process under certain conditions.2. We require a protein that can specifically bind to the metabolites produced in the UMP biosynthesis via de novo pathway, Pyrimidine metabolism, UMP from orotate: step 1/2 pathway.3. The 5-phospho-alpha-D-ribose 1-diphosphate binding site should be located in a region of the protein that is accessible to the ligand.4. For general function, the protein need meet that Catalyzes the transfer of a ribosyl phosphate group from 5- phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP). The designed protein sequence is ",
    "response#1": "MLDVVLANAKESGVMPAAVGGMAIGADPITASIVTLAGGDDVDLKGFMVRKEPKGHGMGQQVEGPVTPGQKVVIVEDVITSGGSALKAVEAVQAFGLEVQYVLAIIDRLAGGAEAFAQKGLELKTLTSY",
    "response#2": "MLDVVLANAKESGVMPAAVGGMAIGADPITASIVTLAGGDDVDLKGFMVRKEPKGHGMGQQVEGPVTPGQKVVIVEDVITSGGSALKAVEAVQAFGLEVQYVLAIIDRLAGGAEAFAQKGLELKTLTG",
    "response#3": "MLDVVLANAKESGVMPAAVGGMAIGADPITASIVTLAGGDDVDLKGFMVRKEPKGHGMGQQVEGPVTPGQKVVIVEDVITSGGSALKAVEAVQAFGLEVQYVLAIIDRLAGGAEAFAQKGLELKTLTPG"
  },
  {
    "reference": "MGGKWSKSSIVGWPKVRERIRQTPPAAIGIGAASQDLDRHGAITSSNTAYTNPDCAWLEAQEEQEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLVYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGIRFPLTFGWCFKLVPVDPEKIEEPTAGENNCLLHPLNQHGMDDPEREVLMWKFDSRLAFHHMARELHPEYFKNC",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have SH3 domain binding within extracellular region, host cell plasma membrane, host cell Golgi membrane to regulate suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I.2. The protein design should include a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Diacidic; necessary for CD4 internalization motif to ensure proper function.3. For general function, the protein need meet that Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD. The designed protein sequence is ",
    "response#1": "MGGKWSKSSLVGWPQIRERMRRAEPAAEGVGAVSRDLEKHGAITSSNTAATNAACAWLEAQEEEGEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPEKVEEANEGENNSLLHPMSLHGMEDPEKEVLQWKFDSRLAFHHVARELHPEYYKDC",
    "response#2": "MGGKWSKSSIVGWPAVRERIRRTDPAAEGVGAVSRDLEKHGAITSSNTAATNADCAWLEAQEEEGEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYNTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVEQDKVEEATEGENNSLLHPMSLHGMEDPEKEVLEWRFDSRLAFHHVARELHPEYYKNC",
    "response#3": "MGGKWSKSSIVGWPAVRERIRRTDPAAEGVGAVSRDLERHGAITSSNINHPSCTWLEAQEEEEGVGFPVRPQVPLRPMTYKAAFDLGFFLKEKGGLDGLIYSKRRQEILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPREVEEANEGENNSLLHPICQHGMEDEHREVLKWQFDSSLARRHMARELHPEFYKDC"
  },
  {
    "reference": "MKILLLILIAYLLGSIQTGLWIGKVFFHTNLREHGSGNTGTTNTFRVLGKTAGTITFLVDMLKGTLAVLLPIWLGVTEVSPLIIGFFAIIGHVFPFFTGFKGGKAVATSAGVLLGFVPLYFVFLLLVFALTLYLTSMISFSSITAAVVGLITLATFPAIHFLLDGYDPIFSAVLIIIALVIIFRHTENIARIRNHRENLVPFGLNLTKQNPNK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that can perform acyl-phosphate glycerol-3-phosphate acyltransferase activity in plasma membrane to support phospholipid biosynthetic process.2. For general function, the protein need meet that Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA)3. The protein design should take into account the physiological conditions of the Lipid metabolism, phospholipid metabolism pathway.4. The protein should be designed to catalyze the following reaction an acyl phosphate + sn-glycerol 3-phosphate = a 1-acyl-sn-   glycero-3-phosphate + phosphate.5. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "MTIIVLATRNAHKVTELRAILGEALDGVEVIGYDGPEPVEDGETFDDNALIKARAALDHTGLPAVADDSGIEVDALGGAPGVLSARWSGRHGDDEANLRLLLDQLADVPDAHRTARFRCAIALVRPDGSEHVVEGVWPGTIARAPHGDRGFGYDPVFVPDGQDERTTAELSPAEKDAASHRGRAFRALLPAVRALVA",
    "response#2": "METIAKQFPLKLLQEDRAITGLKGKVITLDGKALCHALFANDPQFALPINKALIADIKARLEAYDVAEIDYLACHPIDLDLVEEQLQSSTGLDIHTEPELRVALINKVAQICARWEGVIPIHSNHRIHPLSGVAGVKIHPHSCLNCWHIRPSMDEAFPCPFCSTELKAIDS",
    "response#3": "MINPPLAPIFILGTDTGVGKTLVSCALLRAARAMNYHPFCWKPVQTGCERRDDQLIAQAAGVASDDVLCLNPYRLAAPVSPHLAAEQESKIIDIPALRTGFTALSQKAELALVEGAGGLYVPLNDRETYLDLVRHWQLPALVVANARLGMINHTLLTVEAIRQQGLAIAGLVFNHVEPAADSHLEQTNLADIQRLTGLPVLARLPWQADAPLADDLDTLVDRLEPQLDLSALLGI"
  },
  {
    "reference": "MSDLFRMEERRQMPILPTEPARKEGDGGGPQKPDVKRPDTSDLLRRMKRVDPDAARRYRQRSGE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: This may serve to target the modified proteins for degradation by proteasomes.. The designed protein sequence is ",
    "response#1": "VEKAELRLAIADSPQKFETNLQTFLPPLLLKLASPHASVRTAVFSALKNLISRINTLPQVQLPVRALIVQAKEPNLAAQQDSTNVRLYSLLLASKGIDRLSLQDRQQLLPLVVSNISCLTGTVAARMFHILLKLILEWVAPQESSHEQEEFVQFLQLDNDGFSFLMRQFTRFFLLVPSKQVQVSQQPLSRGYTCPGLSLTDVAFFTYDAGVTFNKEQLNKFKKAIFQFVCRGMAATQTIEQSPRMIELMEFLCVVSTDSTNLSDDAAQFMKRFPMPYENEEFITFLQTLYIGNTANGRPPVKAILQEKILSILNRSHFATTKAECISLICSIGLHSSEYKLRSLTLSFIRHVAKLNYKNLNPASSSPSSTDFSTCIVSLIRNNLHAEGWPKLQLGPQTPAFNTAILQRQLQYETLGDILKRDFELVSDLSYIEFLFESLKNDLPQFRSSIQESLLSLVGHLSILPQQSKLKLKNLLRKNLSIDEQQREDNNDAVNSIMALKFVSIKFTNAAFPFHDPEARLFNIL",
    "response#2": "EFYKNALMYLSYCKLETISQEEQASLAYNLCIAALVGENVYGFGDLIANPILKALEGSQHNWLIAFLKAFNIGDIQQFEGLMSQHRDIISTQTAITNNMQKLRQKISILSLLELAFRTPSDKRSISFSKIAQATKLPLGEIEHLLMKSLSLNLIKGSIDQTVEIIHITWVTV",
    "response#3": "VEKAELRLAIADSPQKFETNLQTFLPPLLLKLASPHASVRTAVFSALKNLISRINTLPQVQLPVRALIVQAKEPNLAAQQDSTNVRLYSLLLASKGIDRLSLQDRQQLLPLVVSNISCLTGTVAARMFHILLKLILEWVAPQESSHEQEEFVQFLQLDNDGFSFLMRQFTRFFLLVPSKQVQVSQQPLSRGYTCPGLSLTDVAFFTYDAGVTFNKEQLNKFKKAIFQFVCRGMAATQTIEQSPRMIELMEFLCVVSTDSTNLSDDAAQFMKRFPMPYENEEFITFLQTLYIGNTANGRPPVKAILQEKILSILNRSHFATTKAECISLICSIGLHSSEYKLRSLTLSFIRHVAKLNYKNLNPASSSPSSTDFSTCIVSLIRNNLHAEGWPKLQLGPQTPAFNTAILQRQLQYETLGDILKRDFELVSDLSYIEFLFESLKNDLPQFRSSIQESLLSLVGHLSILPQQSKLKLKNLLRKNLSIDEQQREDNNDAVNSIMALKFVSIKFTNAAFPFHDPEARLFNIF"
  },
  {
    "reference": "MPTVADQMVETLKTAGVRRIWGIVGDSLNGFTDAIRRRGGIDWLHVRHEESGAFAAAGEAEATGELAVCAGSCGPGNLHLINGLFDAHRSRVPVVAIAAHIPSPEIGSGYFQETHPEELFRECSSYCELVSNIAQMPRALEIAIRRSVAERCVSVIVIPGDIALKEIPAVTTPSRDALLPARPRVVPPADAIDKLASLLDGASRITLLCGGGVAGAHDEVVELASRLKAPIVHALRGKEHIEWDNPYSVGMTGLIGFSSGFYAMGACDVLLMLGTDFPYRQFYPQGAKVVQVDIRPEVIGRRTPIELGVIGDVGETLRALLPKVPVRNEDAHLRASLSHYREARTALDKLAHGKPGGRLHPQHVMRVLSEQASADAAFTADVGLPTVWSARYLEMNGKRSLTGSFWHGSMANAMPQAMGIQAACPNRQVISLSGDGGFSMLMGDLLSLRQLNLPVKVVVFNNSSLGFIELEQKSSGFVDFGTGFDNPDFAAMAQAAGIKGIRIEDPADLESGIAAAFAHPGPVLIDAVVERMELAMPPKVTTEMAKGFTLYMIKAVLNGRGDEVLELARANLLR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should contain one or more Thiamine pyrophosphate enzyme central, Thiamine pyrophosphate enzyme N-terminal TPP-binding, Thiamine pyrophosphate enzyme TPP-binding domains that are essential for its function.2. The protein should be designed to catalyze the following reaction a ubiquinone + H2O + pyruvate = a ubiquinol + acetate + CO2.3. The protein must exhibit the following characteristics: A peripheral cell membrane enzyme that catalyzes the oxidative decarboxylation of pyruvate to form acetate and CO(2); It channels electrons from the cytoplasm to the respiratory chain at the cell membrane via ubiquinone..4. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+), FAD, thiamine diphosphate binding.5. The designed protein should be involved in pyruvate catabolic process by localizing to plasma membrane and exhibiting thiamine pyrophosphate binding, lipid binding, magnesium ion binding, flavin adenine dinucleotide binding, ubiquinone binding.6. The protein must bind to thiamine diphosphate in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "TIADLIVESLKNAGVERVYGLPGDSLNGFTDSLRRDGTIEWIHVRNEEVAAFAAGADAHLTGSLAVCAASCGPGNLHLINGLFDCHRNRVPVLAIAAHIPSPEIGTNYFQETHPQNLFKECSHYCEMVGIPEQMPRVLEIALRTAINLSGVSVIVLPGDDRLVELLSHSQAVTLLCGSGCAGAHDQVVALADALGAPVVHALRGKEHVEWDNPFDVGMTGLIGFSSGYHAMLNCDTLVLLGTDFPYRQFYPTDACVIQIDRDPQALGRRVPLDLGVVGDVRETLAAVAAALGDGSPIRPEYVAGLVNRLAAEDAVFTVDVGSPVVWAARYLSMNGRRRLIGSFNHGTMACALPLAIGAQTAFRDRQVVALAGDGGLTMLFGELITLTQNRLPVKVIVFNNSSLNFVELEMKAAGIVNFGTDLHNPDFADVATAMGIFGRRVEHPAQLEQAVADALAHDGPAVVDVVTARQELGRVTSVLHLDVGTPTVWAARYLKMNGQRRLLGSFNHGSMANAMPQAIGAQAACPARQVIALAGDGGFSMLMGDFISLAQLKLPVKIIVYDNASLGFVAMEMKSAGYLDTGTDLHNPDFAAMANAMGILGLRVEDSEGLEGALRTALAHDGPVLVDVLG",
    "response#2": "MTVADLLAKTLASAGVSRIWGVTGDSLNGLNDSLRRFDQIEWMHVRHEETAAFAAGAEAAITGKLAVCAGSCGPGNLHLINGLFDCHRNRTPVLAIAAHIPSSEIGLGYFQETHPQELFRECSVYTELVSNPEQFPAVLERALRAVTRQSGVAVIVLPGDVALGPAPERPAPAPITVRPATPEPDPGEVDARAVRILDGAKKVTILAGAGTQGAHDEVVALADRLAAPIVHALRGKEFLEYDNPYDVGLTGLLGFASGYRAMDAADAVLMLGTDFPYPQFYPDHAKTIQVDIRGSQLGRRHPLDLGLVGDVKATAAALLPRLRRRTDRRHLDDCLRHYRRARRELDDLAVGDSRRRIWAMSSVSGGRRTAVAIGVQAAHPGRQVVALSGDGIGHPQFVARAIDEAAAEDAVFLPDVGSPVMWAARYLHMNGRRRLIGSFSHGSMANAVSQAIGVQAAHPGRQVVALSGDGGLTMMLGELITLVQEELPVKVVVFENSSLNFVELEMKAAGFVNWGTGLRNPDFAEVARAIGMHGARVERSEDLAPALEEAFAHDGPALVNVITERQSVPPAITAEQTVAENIVSVLRHHGVQRVYGLPGDSLNGFTDAIRRDGDIRWMHVRHEEAAAFAASAEAELTGELTVCAGSCGPGNLHLINGLYDANRSRVPVLAIAAHIPTAEIGSTYFQETHPQELFRECSVYVEYVADPVQMPRLLEIAMREAIEKRGVAVLVIPGDVR",
    "response#3": "MTVADLLAKTLASAGVSRIWGVTGDSLNGLNDSLRRFDQIEWMHVRHEETAAFAAGAEAAITGKLAVCAGSCGPGNLHLINGLFDCHRNRTPVLAIAAHIPSSEIGLGYFQETHPDELAIHPQHVARVISEQADDDAVFACDVGTPTIWAARYLKMNGRRRLIGSFNHGSMANALPQAIGAQSAYPGRQVVTLSGDGGLAMLMGELLTLRQLGLPIKIIVFNNGTLGFVEMEMKAAGLIETGVSLDNPDFAGMARSIGMHGVRVTDPGQVDAGVREVLSHPGPALLDAVTARTELPPQVEARQVKGFPIHPQYLARLIDEAAADDAVFIPDVGSPVVWAARYLSMNGRRRLIGSFSHGSMANALPHAIGVQAAQPDRQVVALAGDGGLTMLMGELITLTQNRLPVKLVVFNNSSLNFVELEMKAAGFVNYGTDLVNPDFAQVAQAMGIYARRVERSEDLPEAIADVLAHDGPALLDVVTERQELVRL"
  },
  {
    "reference": "MNTEGLPLKVLSDAVGTTVSLELKNGELYTGTLSEVVDNMGVLLTSARKTTMAGREVDMPKVLVCGTNIVFFQLPDALRCCPPLMKMGKLLPSDLDGRGDGKGFGAHRSRKKPKK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for stability and solubility when expressed in nucleolus, SMN-Sm protein complex where it will be involved in RNA splicing, via transesterification reactions. The designed protein sequence is ",
    "response#1": "IPLGRCGLDLIVQAKSGTGKTCVFTTIALDSLILENATTQVLVLAPTREIAVQIHAVVMAIGSAMEGLECHVFIGGRPISQDKQHLKKCHIAIGSPGRIKQLIEMGALMVSSIRLFVLDEADKLLEDDSSSSFQEQINWIYSSLPANKQMLALSATYPESLAQQLSRYMREPTFVSGDCKGYSPEDLEEKRTSLKKWLEKNHIPVTEQGDAQRTLCVAGVLTIDPPYAPENCSSSNEIILSRIQDLIQGHLSASC",
    "response#2": "SPIQLKAIPLGRCGLDLIVQAKSGTGKTCVFTTIALDSLILENATTQVLVLAPTREIAVQIHAVVMAIGSAMEGLECHVFIGGRPISQDKQHLKKCHIAIGSPGRIKQLIEMGALMVSSIRLFVLDEADKLLEDDSSSSFQEQINWIYSSLPANKQMLALSATYPESL",
    "response#3": "SGDCKAYSPEDLEKRKNSLKKWLEKNHIPITEQRDSRKTLCVAGVLTIDPPYGPENCNSSNEIILSRVQDLIQGHLEASL"
  },
  {
    "reference": "MPKSCAARQCCNRYSSRRKQLTFHRFPFSRPELLREWVLNIGRADFKPKQHTVICSEHFRPECFSAFGNRKNLKHNAVPTVFAFQNPTEVCPEVGAGGDSSGRNMDTTLEELQPPTPEGPVQQVLPDREAMEATEAAGLPASPLGLKRPLPGQPSDHSYALSDLDTLKKKLFLTLKENKRLRKRLKAQRLLLRRTCGRLRAYREGQPGPRARRPAQGS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein's functional design necessitates the inclusion of a HCFC1-binding motif (HBM) motif.2. For general function, the protein need meet that Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1.3. The protein design must be able to perform metal ion binding in order to contribute to positive regulation of transcription by RNA polymerase II. The designed protein sequence is ",
    "response#1": "IGLVVPEITNYGFAVFSHELETLCREAGVQLLISCTDENPGQESVVVNNMIARQVDGLIVASCMHSDADYQKLSEQLPVVLFDRSPSDSALPLVMTDSVTPTAELISRIAPQHADEFWFLGGQPRLSPSRDRLAGFTQGLAQAGITLRPEWVINGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQHHLLDSNIHLASFDDHYLYDSLSLRIDTVQQDNRQLAWHCYDLLSQLIDGR",
    "response#2": "IGLVVPEITNYGFAVFSHELETLCREAGVQLLISCTDENPGQESVVVNNMIARQVDGLIVASCMHSDADYQKLSEQLPVVLFDRSPSDSALPLVMTDSVTPTAELISRIAPQHADEFWFLGGQPRLSPSRDRLAGFTQGLAQAGITLRPEWVINGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQHHLLDSNIHLASFDDHYLYDSLSLRIDTVQQDNRQLAWHCYDLLSQLIDGR",
    "response#3": "IGLVVPEITNYGFAVFSHELETLCREAGVQLLISCTDENPGQESVVVNNMIARQVDGLIVASCMHSDADYQKLSEQLPVVLFDRSPSDSALPLVMTDSVTPTAELISRIAPQHADEFWFLGGQPRLSPSRDRLAGFTQGLAQAGITLRPEWVINGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQHHLLDSNIHLASFDDHYLYDSLSLRIDTVQQDNRQLAWHCYDLLSQLIDGQ"
  },
  {
    "reference": "MPTPRMSMRKLKEVLRLKWACGLTHRQISRAIGISVGAVSKFAAQASQAGLDWAAAEAMSDDELDARLRPAATNAAATTTRRIEPDYTALHRELRRKGVTLQLLWEEYAEANPGQRTYRYTQFCQKYKDWAKSIKRSMRQQHRAGEKLFADFAGPTVPVLASDGGVEFEAHVFVAVLGASNYTFACATRTETMADWIGSLCDALEFIGGVPELLVPDNPKALIARPDRYEPGLGTTTQDFVNHYGTAMLPARPRKPQDKAKVEVGVQIVERWVLARLRHYRFYSLAELNKAIAELIADLNQRPFKRLEGNRREWFERLDQPVLRPLPVRRYEIATFQKCRVNIDYHVDVGGHYYSVPHSLARQEVWARITRHGVEILHGGKRVAAHARSRLKGKHTTIAEHMPAAHRAHMEWTPGRLLNWGASVGPGAEAVVRHLLTNKPHPEMGYRACLGLLSLARKYGKHRLEAACQRALKIGSPTRRSVLSILEAGLDLQPSLPTTPAEWRSPEHENVRGPDYYH",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design must be able to perform DNA binding in order to contribute to DNA integration, transposition.2. The presence of Integrase catalytic, HTH IS408-type domains is critical for the interaction of the designed protein with its intended targets.3. For general function, the protein need meet that Required for the transposition of the insertion element.4. Incorporation of the H-T-H motif domain in the designed protein should not interfere with its overall stability and folding. The designed protein sequence is ",
    "response#1": "HMGSLRVACYTAPALSFMSGVIQTFIADRPDVSVYLDTVPSQTVLELVSLQHYDLGISILAGDYPGLTTEPVPSFRAVCLLPPGHRLEDKETVHATDLEGESLICLSPVSLLRMQTDAALDSCGVHCNRRIESSLALNLCDLVSRGMGVGIVDPFTADYYSANPVIQRSFDPVVPYHFAIVLPTDSPPPRLVSEFRAALLDAL",
    "response#2": "HMGSLRVACYTAPALSFMSGVIQTFIADRPDVSVYLDTVPSQTVLELVSLQHYDLGISILAGDYPGLTTEPVPSFRAVCLLPPGHRLEDKETVHATDLEGESLICLSPVSLLRMQTDAALDSCGVHCNRRIESSLALNLCDLVSRGMGVGIVDPFTADYYSANPVIQRSFDPVVPYHFAIVLPTDSPPPRLVSEFRAALLDAL",
    "response#3": "HGRLPLVMRQSHAGGEKLFVDYAGDKVAVVDRKTGVVRDAHIFVAVMGASSLSFALATWSEQLPDWIEAHNAAYRFFGGVTQLLVSDNTKCAVIKACHFDPMVNRSYTDMARHYSTAVFPARPRKPRDKAKVENCVGIVERWLLGRLRNRTFYSLADLNKAIADLITRLNDERVIRQYGKTRRALFDELD"
  },
  {
    "reference": "MPEGLIVKALSGFYYVQSEGALIQCRGRGVFRKRKQTPLVGDYVEFEAENETDGYVMAIKERKNQLNRPPIANVDQAILVFSAKEPDFHTLLLDRFLVHIEQHDIKPVLIISKTDLLEASERDALTRQVAMYEQIGYPVLYTSTKQADDPSELLPLLANQVSVIAGQSGVGKSSLLNALAPEAKIETNEISRHLGRGRHTTRHTELLPIGEGLVADTPGFSSLEFTDMEAEDLRFCFPEFLSLMDDCKFRGCLHDKEPKCAIKDAVETGEVDSFRYKHYLQFLHEIQDQKRRY",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must bind to Zn(2+) in order to perform its enzymatic function.2. The designed protein must be optimized for rRNA binding, GTP binding.3. The protein should be able to bind Zn(2+) ligand in a variety of conditions4. For general function, the protein need meet that May play a role in the assembly of ribosomal proteins into the subunit; One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit; Circularly permuted GTPase that catalyzes slow GTP hydrolysis, GTPase activity is stimulated by the 30S ribosomal subunit. The designed protein sequence is ",
    "response#1": "VKERTNELVRPPISNVDQAVLVFSAAEPTFSTSLLDRFLVLVEANHIEPIICITKMDLLKTDEERETIMAYADDYRQIGYEVHLTSTIEGDGIEKLTPHFHNKITVFAGQSGVGKSSLLNAMSPELALKTDDISSHLGRGKHTTRHVELIRTANGLIADTPGFSSLETNELVRPPISNVDQAVLVFSAAEPTFSTSLLDRFLVLVEANHIEPIICITKMDLLKTDEERETIMAYADDYRQIGYEVHLTSTIEGDGIEKLTPHFHNKITVFAGQSGVGKSSLLNAMSPELALKTDDISSHLGRGKHTTRHVELIRTANGLIADTPGFSSGSGKANVDYLAIQFAAKHPNIDFERINLLLLTAFYYKIKPIVIVNKIDYLTEEELVELKEKLSFLERISVPMFLISCYQNIGLEEVENFLKDKITVIGGPSGVGKSSFINFLQSERILKTGEISERLQRGKHTTRDSNMIKMKAGGYIIDTPGFVKERTNELVRPPISNVDQAVLVFSAAEPTFSTSLLDRFLVLVEANHIEPIICITKMDLLKTDEERETIMAYADDYRQIGYEVHLTSTIEGDGIEKLTPHFHNKITVFAGQSGVGKSSLLNAMSPELALKTDDISSHLGRGKHTTRHVELIRTANGLIADTPGFSSLEVVPRRNLFYRQDEIRTKSFAANLDHVLILIAAEPEFSEHQLARALIAAEAERITPIIALNKSDLAEPFERAWNKLAPYRRMHHGVLPLSLKASGEADYASLMKLLAGKSTLVLGPSGAGKSTLINLLVPGATALTGEISQALNSGKHTTTSTTWYWVDEARTTSLIDSPGFQEFG",
    "response#2": "RQDEIRTKSFAANLDHVLILIAAEPEFSEHQLARALIAAEAERITPIIALNKSDLAEPFERAWNKLAPYRRMHHGVLPLSLKASGEADYASLMKLLAGKSTLVLGPSGAGKSTLINLLVPGATALTGEISQALNSGKHTTTSTTWYWVDEARTTSLIDSPGFQEL",
    "response#3": "LSKQSHILAANLDQCMLIVTINYPETSTIFIDRFLATAEAYRVPVKLIFNKTDRYNEDDTRYMDALINLYTYIGYPCFKVSALNNIGTDEVKKDLEGKVTLLSGNSGVGKSTLINAILPEQTLKTGEISDYHNKGMHTTTFSEMFPVDGGGYIIDTPGIKGANVSHVLLVISAVEPDFSLELLDRFLTFFSWQKVNVTIYLSKADLISEQRLNEIKESLDYYQKIGYPVFIDYHHLEEKIGDMIKDNQIWTLAGQSGAGKSTLLNHLKKDANQTTGAISTSLNRGKHTTRKVELFKLGHGFLADTPGFR"
  },
  {
    "reference": "EGKAIGLAKPRMDST",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a specific function within the extracellular region. The designed protein sequence is ",
    "response#1": "PGDGGPPGQPGPDGPPGAPGNAGEDGKPGDQGPRGPPGIPGAPGQPGRPGPPGEPL",
    "response#2": "PGDGGPPGQPGPDGPPGAPGNAGEDGKPGDQGPRGPPGIPGAPGQPGRPGPPGEPI",
    "response#3": "PGDGGPPGQPGPDGPPGAPGNAGEDGKPGDQGPRGPPGIPGAPGQPGRPGPPGEPI"
  },
  {
    "reference": "MMKDSSLKIFSGNSNPALAKEICKSLGVPLAEADVISFPDGESFVRINENVRGADVFIIQSTCNPANQNLMELFIMIDAARRASANRITAVIPFYGYARQDRKDQPRVPITSKLVANLLVSAGTDRVLTVDLHAQQIQGFFDIPVDHLYASPVLFQYLKDIDTKNLTLFSPDVGGMKMASAYASMLQVPLGFIAKRRTSAETVEATSLVGETEGRDILLVDDMTETAGTLCAAAKLLKEHGAKSVMAAVSHGVLNEMGYERLASGVIDQLITTNTTPVETRPGVPITVLSVADLLGEGIRRIHDNESVTGLFDIKGF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be involved in 5-phosphoribose 1-diphosphate biosynthetic process, ribonucleoside monophosphate biosynthetic process, nucleotide biosynthetic process, phosphorylation by localizing to cytoplasm and exhibiting ATP binding, kinase activity.2. A protein that is capable of catalyzing reactions within the Metabolic intermediate biosynthesis, 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1, 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis pathway with high efficiency and specificity.3. The designed protein should contain one or more Ribose-phosphate pyrophosphokinase N-terminal domains that are essential for its function.4. The designed protein must have a active site that is structurally stable and resistant to denaturation.5. For general function, the protein need meet that Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P).6. The D-ribose 5-phosphate, Mg(2+), ATP binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGADRGLCLDLHAAQIQGFFDVPVDHLYAAPVFYQHLRQKNLKELVVVSPDVGGMKMASAYAQTLGAGLAIVAKRRISATETQALYVVGEVKDRDVLLVDDMTETAGTLSSAAKTLREAGARTIYACVSHAILNEKGLERLKSSGIEELITTDTIPLADDQRFERLTVLSVAPLFAEAIRRTHTGLSVTGLFE",
    "response#2": "MKIFAGNATPKLANLIAKRLYTQLGEATVSRFSDGEISVQINENVRGDDIFIIQSTCAPTNDNLIELVVMIDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKIVADFLSSVGADRIMSVDLHAQQIQGFFDIPVDHAVHILADYFVAKQLTNPVVVSTDLGNAKRARNFAELLNCPLALIEKRRTGNDDRTSVLNLIGTVKGHQAVLIDDEIDTGGSMVQAAQRVADEGVTEIYAACIHGILSGPAIERLKKSPIQELVITDTIPLSEKANNCKNIQVLSIAGLLGRAIESIHEETSISSLFV",
    "response#3": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGADRLMSVDLHASQIQGFFNIPVDNAVHILADYFVAKQLTNPVVVSTDLGNAKRARNFAELLNCPLALIEKRRTGNDDRTSVLNLIGTVKGHQAVLIDDEIDTGGSMVQAAQRVADEGVTEIYAACIHGILSGPAIERLKKSPIQELVITDTIPLPHEKQLPNLTVLTVAGLIGRAIEQIHEETSVSDLFK"
  },
  {
    "reference": "MKLVLATHNPHKVEELRRILGDALDGIELVGYDGPEPVEDGESFEANALIKARAASAFTGLPAIADDSGISVDALGGSPGIHSARYAGGRDDAANVELVLANLGDEANRAAHFTCAAALVVPPAVGAIGPDGPADEAGALEFVEVGIWRGSVLHEPAGRNGFGYDPIFKPAGMSVSSAVLRSEEKNAVSHRALAFTAIMPTVRAQLLGE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein requires Mg(2+) to activate a critical catalytic site.2. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+).3. The protein should be able to bind substrate, Mg(2+) ligand in a variety of conditions4. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.5. The protein design must be able to perform ribonucleoside triphosphate phosphatase activity, dITP diphosphatase activity, XTP diphosphatase activity, metal ion binding in order to contribute to nucleotide metabolic process, purine nucleoside triphosphate catabolic process. The designed protein sequence is ",
    "response#1": "MKKLVFATNNQHKLEEVRAILGKRFELLSLKDIGCTDELPEEQDTLEGNASQKAQYVYENYQLDCFADDTGLEVMALDGAPGVYSARYAGPEKDAAANLQLLLDRLNGVENRKARFRTVISLMVNGKEHLFEGICEGRITTEKRGTDGFGYDPLFIPDGYDQTFAELPMAVKNQISHRARAVAKLVDFLNQTK",
    "response#2": "MKIVIATDNEGFKQEIASILGEDKYELLSLKEMGCYSEVEETGQTFEENAIIKAKAVYEATGLLTLADDSGLEVDVLGGAPGVHSARYAGEGASYQQNNDKLLDLLEGVPKEKRTARFRCVIAFVTPSGEKFMAQGICEGEITEQGSGEGGFGYDPLFLVPEYGKTMAELSLEEKNRISHRGQAFRKAAEVLKGILG",
    "response#3": "MAIFIDTSILLRFLVGDKGAEAEQAKQVIDAVKTLPSHIVTSQITLAETYWVLTNVYKKPQPEAIAFLKTLLENPQVIVLPITLDTALDAWKLFVEHPNFSFVDATSFAVMRRLKIKRAFSFDRDFEKAGFIVAPSVEERE"
  },
  {
    "reference": "MAQINENYLKLPGSYLFSEIARRVNEFKVQNPDADIIRLGIGDVTRPLAPVVVEAMKQAVEEMGRAETFRGYGPEQGYDFLIEKIIANDYAPRGVQLGMDEVFVSDGAKSDTANFQEIFGVDNIMAVTDPVYPVYVDSNVMAGRTGNYDTEKGQYGRIIYLPCTEEGDMKPELPTAPVDMIYLCFPNNPTGMTLTKEELKVWVDYARENKAIILFDSAYEAFIREEGVPRSIYEVEGAREVAVEFRSFSKTAGFTGTRCAYTVVPKDIMIYDSTGEGHSLNKLWLRRQTTKFNGVSYPVQAGAAAVYTEEGKKQIQATIDYYMENARIIREGLQEAGFKVFGGVNAPYIWMKTPGTMGSWEFFDKLMTEAHVVGTPGAGFGANGEGFFRLTAFGTRENTEKAIERIKARMK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for activity in L-lysine biosynthesis via DAP pathway, LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (aminotransferase route): step 1/1 pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The protein must bind to pyridoxal 5'-phosphate in order to perform its enzymatic function.3. The protein should have a fast turnover rate for (2S,6S)-2,6-diaminoheptanedioate + 2-oxoglutarate = (S)-   2,3,4,5-tetrahydrodipicolinate + H(+) + H2O + L-glutamate.4. The designed protein should be optimized for pyridoxal phosphate binding, L,L-diaminopimelate aminotransferase activity activity in order to improve lysine biosynthetic process via diaminopimelate, diaminopimelate-aminotransferase pathway. The designed protein sequence is ",
    "response#1": "MNSCYVYSHSYLTERARRFTKALDGAGKGKSLVAFAVKANPSQAILASFAKEGLGADVVSAGEIRRAVHAGIPPERIVFSGVGKTAEEMRYALEIGIGQFNIESVSEIEMLAEVATSLGKKAAVALRINPDVDPHTHAKIATGKADTKFGIAAEDALSAYEKLASYPSLKIQGIASHIGSQITDLAPFEAAAERIYEIITALEKAGHAIETADLGGGLGVRYKDDQPEPPSVEAYGEMIKRVTKGWNCRLIFEPGRSLIANAGVLLSKVIRIKESKTARFVILDAAMNDLVRPTLYDAYHEIKAVTPSAQTYQADIVGPVCETGDIFARNRSISAVKADDLMAIMSAGRI",
    "response#2": "LIRERARGFKNTFDELGIKAQVAYASKAFSTVAMIQLAEEEGLSLDVVSGGELYTALVAGFPVHKIHFHGNNKSRAELEMALEHQIGCIVVDNFHELDLIDSICSEKNVKTNILLRVTPGIEAHTHDYILTGQEDSKFGFDLQNGQAEKALQIALNSNFVEVLGVHCHIGSQIFDTTGFVLAARKIFEKLKEWKDRLSYEPKVLNLGGGFGIRYTEEDDPIPASQYVKEIINEVKKQVSAYSMKMPEIWIEPGRSLASYEPKVLNLGGGFSRAGVTLYRVAAVKRGVRRVFVAVDGGMSDNPRPALYGSRYAVRLVRRGGRRAPVTVVGRHCEAGDVLAEDVPLPEDVRAGDLLAVPVTGDAGTTLYQIGSRKDVPNVRHYVAVDGGMSDNIRPALYNAKYEAVLANKPLAKADETVSIAGKCCESGDMLIWDLPLPKADSDDILAVFCTNTCCFVNRVTGVKSNGTKNFIVVDGSMAELIRPSLYGAYQHIELVSPSPDAEVATFDIVGPVCESADFLGKDRELPTPDKGAGLVVHDAY",
    "response#3": "MKLQLINLGIGDTTQPIPLYISEAMQNFAKQLASEKTYRGYGTEQGSILLREAIAEQYYQGKIDPQEVFVSDGSKCDVGRLQILFGSDATIAVQNPTYPAYVDTGVINGQASFFQTSTKQYQRITYMSCLPENNFFPDLANLPKTDLIYFCSPNNPTGSAATNEQLRELVQFAKKRQSIIIFDAAYASFVRSSHIPRSIYEIEGAKEVAIEVGSFSKMIGFTGVRLGWSVVPKQLRFEDGHSVQQDWERIVCTFFNGASNIAQAGGLAALQKEGLQAIDELSSYYMKNSNILKKAFEECGYKVYGGENVPYLWVHFPQLTSWEAFEILLKQSQLVSVPGSGFGSAGEGFLRFSAFGKQSDITVALPRLARVLGE"
  },
  {
    "reference": "MKAVVFAYHDMGCTGTLALLEAGYDIAAIFTHPDTAGENNFFGSVARIAAERGIPVYAPDDVNHPLWVDRIRALAPDVIFSFYYRNLISDEILGLAAKGAFNLHGSLLPAYRGRAPLNWVLVNGETETGVTLHRMVHRADAGAIVAQQKVAIDADETALQLHQKLNATAQTLLRDALPAILGGTFSETAQDESKASTFGRRAPEDGRLDWNKPARQLHNLVRAVTDPWPGAYSFAGVSKFIVWKSRVRDDIPAAKPGTVISVSPLIVSCGEQALEIVTGQTDNGLYVQGTQLAQSLGLVTGALITSAPVVAIKRRTRVLILGVNGFIGNHLTERLLKDDNYEIYGLDIGSDAISRFLDNPRFHFVEGDISIHSEWIEYHIKKCDVVLPLVAIATPIEYTRNPLRVFELDFEENLKIIRDCVKYDKRIIFPSTSEVYGMCTDKNFDEDTSNLVVGPINKQRWIYSVSKQLLDRVIWAYGEKEGLRFTLFRPFNWMGPRLDNLNAARIGSSRAITQLILNLVEGSPIKLIEGGKQKRCFTDISDGIEALFRIIENKDNRCNGEIINIGNPDNEASIRELAEMLLASFERHPLRDKFPPFAGFREVESSSYYGYGKGYQDVEHRKPSIRNAKRCLNWTPTVKMELTIDETLDFFLRTVELSEQDS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must have a Proton acceptor; for decarboxylase activity active site that is highly conserved among related enzymes.2. The designed protein should interact with enzymes and substrates within the UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate: step 3/3 pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "RVLILGVNGFIGNHLTERLLQDDNFEVFGLDISADAISRFIGHPRFQFVEGDISIHSEWIEYHIKKCDVILPLVAIATPIEYTRNPLRVFELDFEENLKIIRDCVKYDKRIIFPSTSEVYGMCDDDSFDEDHSRLVVGPINKQRWIYSVSKQLLDRVIWAYGDKQGLRFTLFRPFNWMGPRLDNLNAARIGSSRAITQLILNLVEGSPIKLIDGGSQKRCFTDISDGVEALFRIIENRAGNCDGQIINIGNPHNEASIRDLAEQLLASFERHPLRAQFPPFAGFREVESSSYYGKGYQDVEHRKPSIRNARRLLEWTPQVAMETTIDNTLDFFLRR",
    "response#2": "VLILGVNGFIGNHLTERLLADDNYEIYGLDIGSDAISRFLDNPRFHFVEGDISIHSEWIEYHIKKCDVVLPLVAIATPIEYTRNPLRVFELDFEENLKIIRDCVKYNKRIIFPSTSEVYGMCTDKNFDEDNSNLVVGPINKQRWIYSVSKQLLDRVIWAYGDKEGLKFTLFRPFNWMGPRLDSLNSARIGSSRAITQLILNLVEGSPIKLIEGGKQKRCFTDISDGIEALFRIIENKGGRCDGQIVNITPEDGRLDWSRPARELHNLVRAVSDPWPGAFSYAGATKFIVWTSRLHADAQGAKPGTVLSVSPFIVACGEGALEIRTGQTERGVYMQGTQLENTDFRQ",
    "response#3": "VLILGVNGFIGNHLTERLLADDNYEIYGLDIGSDAISRFLDNPRFHFVEGDISIHSEWIEYHIKKCDVVLPLVAIATPIEYTRNPLRVFELDFEENLKIIRDCVKYNKRIIFPSTSEVYGMCTDKNFDEDNSNLVVGPINKQRWIYSVSKQLLDRVIWAYGDKEGLKFTLFRPFNWMGPRLDSLNSARIGSSRAITQLILNLVEGSPIKLIEGGKQKRCFTDISDGIEALFRIIENKGGRCDGQIVNIG"
  },
  {
    "reference": "MSAFDPQAHSPPRCGPQFPSIGQEPPEMNIYCDSIFHPQTMPSPQRPSNFETADYSTTANPYLWLNGPSITPPPYLPGSNTSHFMPQSYGMQRQLLPNMHGLGGSDLGWLPIPSQEELMKLVRPPYSYSALIAMAIHGAPDKRLTLSQIYQYVADNFPFYNKSKAGWQNSIRHNLSLNDCFKKVPRDEDDPGKGNYWTLDPNCEKMFDNGNFRRKRKRKSDVSPNGQISSDKPEGSPLNESPKNGEHHDMLGNSSPGTDDSSEKRSPPPSITPCLNNFLSSMTAYVNSANPVSRSVPLGLTNEPSDRMGQNMVGLNSYTPLSNMPSHGGSDWSSTISSSPFGYSSSVFNQFTPHFYNTINANNTLYNREGTEV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Also plays a role in differential adhesion, which limits cell mixing as primary germ layers become specified .; Activates ectoderm, in addition to inhibiting mesoderm and endoderm formation; required at the blastula stage for normal formation of both the central nervous system and epidermis, the two early derivatives of the ectoderm; In addition, also required to maintain the regional identity of the animal cells of the blastula, the cells that are precursors of ectodermal structures The designed protein sequence is ",
    "response#1": "PNLTVSFFLRTREPAGLLLQFANDSVASLTVFLSEGQIRAEGLGHPAVVLPGRWDDGLPHLVMLSFGPDQLQDLGQRLYVGGRFYPDDTQLWGGPFRGCLQDLQLNSC",
    "response#2": "SLSGLTLAFRTRDSEAGLLRAVSAAGAHSNIWLAVRNGSLAGDVAGSVLPAPGPRVADGAWHRVRLAREFPQAAASRWLLWLDGAATPVALHGLGGDLGFLQGPGAVPLLLAENFTGCLGRVALGDC",
    "response#3": "VAEATFREGPPAVFTGHNVSSSLSGLTLAFRTRDSEAGLLRAVSAAGAHSNIWLAVRNGSLAGDVAGSVLPAPGPRVADGAWHRVRLAREFPQAAASRWLLWLDGAATPVALHGLGGDLGFLQGPGAVPLLLAENFTGCLGRVALGDFPLPLAPPRSGTVSGAREHFVAWPGSPAVSLGN"
  },
  {
    "reference": "MELAYMAKLPKEEFEERRTRVFAQMQPNSALLLFSEIEKRRNNDCTYPFRQDSYFWYLTGFNEPNAALLLLKTEQVEKAIIFLRPRDPLLETWNGRRLGVERAPQQLNVNEAYSIEEFATVLPKILKNLTALYHVPEIHTWGDTLVSESAVNFSEILDWRPMLSEMRLIKSPNEIRLMQQAGQITALGHIKAMQTTRPNRFEYEIESDILHEFNRHCARFPSYNSIVAGGSNACILHYTENDRPLNDGDLVLIDAGCEFAMYAGDITRTFPVNGKFSQPQREIYELVLKAQKRAIELLVPGNSIKQANDEVIRIKTQGLVDLGILKGDVDTLIEQQAYRQFYMHGLGHWLGLDVHDVGSYGQDKQRILEIGMVITVEPGIYISEDADVPEQYKGIGVRIEDNLLMTEYGNKILTAAVPKEIADIENLMNF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Mn(2+) binding.2. We need a protein that can perform Release of any N-terminal amino acid, including proline, that   is linked to proline, even from a dipeptide or tripeptide. with high efficiency.3. The protein requires Mn(2+) to activate a critical catalytic site.4. A protein that contributes to proteolysis by functioning in cytosol with metalloaminopeptidase activity, manganese ion binding, peptidase activity, aminopeptidase activity. The designed protein sequence is ",
    "response#1": "NMARQLQDTPSDQLYPEEFVKRFEKAATGLGVKITVLKQADLIKKKMGLLLGVNKGSEREARLLVISYNNNKKSSETLALVGKGITYDSGGMNIKTGDYMRGMKYDMSGAAIVCSTVLALAKNKVKTNVVAVAALTENLPGPHAQRPDDIQTAYNGKTVEIDNTDAEGRLVLADAISYAAKDLKATQIIDVATLTGLMSYILSTTYTGIFSTCDMAWDAFKKAACCAGEPVWRLPMHPDYLKPLESKLADLQNSTSVKGAGSSRAACFLAEFREGVPLIHCDIASTASIQDLGQGVLVRTV",
    "response#2": "NMARQLQDTPSDQLYPEEFVKRFEKAATGLGVKITVLKQADLIKKKMGLLLGVNKGSEREARLLVISYNNNKKSSETLALVGKGITYDSGGMNIKTGDYMRGMKYDMSGAAIVCSTVLALAKNKVKTNVVAVAALTENLPGPHAQRPDDIQTAYNGKTVEIDNTDAEGRLVLADAISYAAKDLKATQIIDVATLTGLMSYILSTTYTGIFSTCDMAWDAFKKAACCAGEPVWRLPMHPDYLKPLESKLADLQNSTSVKGAGSSRAACFLAEFREGVPLIHCDIASTASIQDLGQGVLVRTR",
    "response#3": "LQHAMDACRVIKDEHESEAQIAGLFIDVCLSLRSRGTAYQTIAASGPNGATLHYTRNNEPLAGRQMVVLDAGAEWDCYASDVTRSFPIPSSVSGKENSGKGDWPSREAEEVYAIVQRMQEESISRVREGAMFFSIHQHAHSIALDELLRLGILRIPHGSSKADLVKAEATALFFPHGLGHHLGLEVHDVAPDSGTVPVTSSSSSSSSRVTAVTAQCQDGLMSVTEHRPPCTLSAPPLAAGMVITVEPGIYFNRLAIDQARAERDKPGSKGRFVDFDVVERYMAVGGVRIEDDVLVTE"
  },
  {
    "reference": "MNVLIIGSGGREHALAWKVAQDPRVAKVFVAPGNAGTATEAKCENVAIDVLALEQLADFAAKNVQLTIVGPEAPLVAGVVDLFRERGLDIFGPTAGAAQLEGSKAFTKDFLARHRIPTAAYRNFTEVEPALAYLHEQGAPIVIKADGLAAGKGVIVAMNLDEAEAAVRDMLAGNAFGDAGSRVVIEEFLDGEEASFIVMVDGQNVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTAEVHQRVLDEVIYPTVRGMAEEGNVYTGFLYAGLMIDKSGAPKVIEFNCRFGDPETQPIMVRLESSLVLLVEAALAKALDKVEATWDPRPTVGVVLAAGGYPGDYAKGEVIEGLAEAAALDGKVFHAGTALKDGQVVTSGGRVLCATAIGESVSAAQQQAYRLAEKIRWNGCFYRKDIGYRAIARERGES",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. We are looking for a protein with broad substrate specificity for 5-phospho-beta-D-ribosylamine + ATP + glycine = ADP + H(+) +   N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate.2. The designed protein should have a binding site that is specific to the target Mg(2+), ATP ligand.3. The designed protein must possess ATP binding, phosphoribosylamine-glycine ligase activity to support purine nucleobase biosynthetic process, 'de novo' IMP biosynthetic process under certain conditions.4. Mg(2+) is required for the proper folding and stability of the protein.5. A protein that can modulate the overall flux of metabolites through the IMP biosynthesis via de novo pathway pathway, enhancing or suppressing its activity as needed.6. The designed protein should contain one or more ATP-grasp domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MRILVIGSGAREHALLRGLSTDPATTELHVAPGNAGLGSIATVHPGIKADDPEAVTALAKELNSDLVVIGPEIPLVAGVADALRAAGIAVFGPNKDAARIEASKSFSKSFMDRQGIPTARWSSFTDPQEACRYIRGADFPALVVKASGLAAGKGVIVTQDKEEACKAVEDIMKDKAFGSAGETVVVEELLEGEEVSCLCFTDGITIAAMPPAQDHKRLQDGDKGPNTGGMGAYCPTPQVPEEMMQQIRETVLQKTVDGMREEGTPYVGVLYAGLMLTPQGLKVLEFNCRFGDPEYALTVVLASYNYPEAPRTGDVIRNADADNVLHAGTALNAEGELVSAGGRVLNVIGVGETLEAARDNAYTTIKDIELEGSHYRSDIALAAFCVGVVMASRGYPLTSSKGDIISGLPSVTKGMVFHMGTKLNNYGEVVTNGGRVLIVVTTHQDLVLAAARATMACGRIIFNGAQFRTDIAHRGLFSVVAVTVVMASPGYPGAYKKGIEITGLSQVEDLGPQVFHAGTALKEGGGVVTSGGRVLTVTAVMPSLDSALHKANQGVAAVGFPGAVYRRDIGHIG",
    "response#2": "MKVLVVGTGAREHAIADALKDDVELYCYMSKVNPGISKIAEFAQGDEGEIEKVAKFAVDNNIDIAFIGPEAPLGKGIVDELEKNGISCVGPSQSAARIEASKSFSKSFMDRQGIPTARWSSFTDPQEACRYIRGADFPALVVKASGLAAGKGVIVTQDKEEACKAVEDIMKDKAFGSAGETVVVEELLEGEEVSCLCFTDGITIAAMPPAQDHKRLQDGDKGPNTGGMGAYCPTPQVPEEMMQQIRETVLQKTVDGMREEGTPYVGVLYAGLMLTPQGLKVLEFNCRFGDPEAVTVVMASPGYPGAYKKGIEITGLSQVEDLGPQVFHAGTALKEGGGVVTSGGRVLTVTAVMPSLDSALHKANQGVAAVGFPGAVYRRDIGHVEVISAAVTVVMASPGYPGAYKKGIEITGLSQVEDLGPQVFHAGTALKEGGGVVTSGGRVLTVTAVMPSLDSALHKANQGVAAVGFPGAVYRRDIGHVEVISA",
    "response#3": "MKVLIVGSGGREHAIAWKISQNSKVDKIFAASGNAYNKVIKNCENINLKTSDDILNFAIKEKVDLTIVGSEELLVDGIVDKFQENNLTIFGPNKEAAMLESDKEWSKEFMNEFNIPTAKWRSFNDVKQATDFVYSADYPALVVKASGLAAGKGVVVASNKQEACLALDAILTENRFGKAGQTVVVEELLQGEEVSVLCFTDGVNINVMLPSQDYKRAYNQDKGPNTGGMGAFCPCYNMPQNELDEAKNILQRAVDGLRNRGTPFVGVLYAGLMVTNDGIRVLEFNCRFGDPEYALTVVLASYNYPEAPRTGDVIRNADADNVLHAGTALNAEGELVSAGGRVLNVIGVGETLEAARDNAYTTIKDIELEGSHYRSDIALAASVCKYLVPPGYPEKGERGLVVTVPAEAAGTECFLGSLALQGDHLVTEGSRTLAVTGFGNTLDEAEARAESWIASVGGGLVHRGDIGRACVGVVMASRGYPLTSSKGDIISGLPSVTKGMVFHMGTKLNNYGEVVTNGGRVLIVVTTHQDLVLAAARATMACGRIIFNGAQFRTDIAHASVCKYIVPDGYPETSHAGETIEVDEKTIEDLGAKVFYAAVGLEDDEIHLSGSRALGIVASGDSIEEAEKIAEKACACIKGNVYHRSDVGTTDALTVVLASYNYPEAPRTGDVIRNADADNVLHAGTALNAEGELVSAGGRVLNVIGVGETLEAARDNAYTTIKDIELEGSHYRSDIVVSCVGVVMASRGYPLTSSKGDIISGLPSVTKGMVFHMGTKLNNYGEVVTNGGRVLIVVTTHQDLVLAAARATMACGRIIFNGAQFRTDIAH"
  },
  {
    "reference": "MKVQKKDIRALSKQELQDFFVAQGEKSFRGTQVYEWLWSKAAHNFADMTNLSKTTRRMLEDNFVINHVRVDKMQRSKDGTIKNAVKLHDGLTVESVLIPTYSRTTACVSSQVGCSLDCKFCATARLKRMRNLNPDEIYDQVVAIDQESRLYNNIPLSNIVFMGMGEPLMNYKNVLKAIDKITSSEGLGMSPRRITVSTSGVPKMIKKLADDEVKFNLALSLHSAIDEVRSRIMPFNTTFPLADVKEALLYWHQKTKKRVTYEYIIWKDINDKKEDIDALVKFCKLIPCKVNIIEYNPIDDGMFQQADERVTNAYQNALERNHITVTVRKSRGKDIDAACGQLANKSS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs..2. The designed protein must have a S-methylcysteine intermediate, Proton acceptor that is structurally stable and resistant to denaturation.3. The designed protein should be involved in rRNA base methylation by localizing to cytoplasm and exhibiting metal ion binding, rRNA (adenine-C2-)-methyltransferase activity, tRNA (adenine-C2-)-methyltransferase activity, rRNA binding, 4 iron, 4 sulfur cluster binding.4. The [4Fe-4S] cluster, S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions.5. The designed protein must have at least one Radical SAM core domain. The designed protein sequence is ",
    "response#1": "EEDRGTLCISSQVGCTLTCSFCHTGTQKLVRNLTAGEIVGQILVARDDLGEWGHKADGKRLVSNIVLMGMGEPLYNFEGVRDAMKIAMDGEGLSLSRRRITLSTSGVVPEIGRAGEEIGCMLAISFHATTDAVRDTLVPINRKWNIEKLIQACRDYPRLSNAERITFEYVMLKGVNDSDADARRLVQLIAGIPAKINLIPFNPWPGAPYERSDWGRIEAFAEIVNRAGYASPVREYKGRADIMEDKRPLIHGLSKSEIGQIVASLGQPSYRATQIWEWLYKRMAFEWNAMSNLPLALREHLDRELAICPGTLTARSADASGSEKLLLTLRDGECVEQVLIPAPGRKTVCVSSQVGCRFKCAFCASGQAGFRRNLEAGEIVAQTLWAALIWERRPSNVVFMGIGEPLDNYDNVLKAIRIINDSEGLGIGARKITISTSGIIPGMERLAGEGLQIELSVSLHAPTQELRSRLMPVARLYPLDRLIRACRSYFEKTKRLITFEYVLIRGTNDQASHARKLAELLSDFPCRVNLLLLSPVPEFKGTSSSPDAANAFIKTLSRAGINATLRAPRGLSVNAACGQLRSSRAR",
    "response#2": "NSICISTQVGCRMGCKFCASTLGGRVRDLTCGEILSQVIIGRKICGDRISNIVLMGSGEPLDNFDNVMKFLELVNADYGLNIGQRHITLSTCGIVPKIIELADKEMSITLAISLHAFSDEKRKEIMPIANKYSIIEILDACRYYINKTGRRITFEYSLVSGVNDGKEDARSLAKILKGMLCHVNLIPVNEIKENTLKRPSKKVIEEFEEERRKK",
    "response#3": "EEDRGTLCISSQVGCTLTCSFCHTGTQKLVRNLTAGEIVGQILVARDDLGEWGHKADGKRLVSNIVLMGMGEPLYNFEGVRDAMKIAMDGEGLSLSRRRITLSTSGVVPEIGRAGEEIGCMLAISFHATTDAVRDTLVPINRKWNIEKLIQACRDYPRLSNAERITFEYVMLKGVNDSDADARRLVQLIAGIPAKINLIPFNPWPGAPYERSDWGRIEAFAEIVNRAGYASPVSRTATLDDVAGKLPD"
  },
  {
    "reference": "MEEIKALYVDLNKLRNIGIMAHIDAGKTTTTERILYFTGRKHQIGSVDDGTATMDWMVQEKERGITITSAATTCLWKEHRINIIDTPGHVDFTIEVERSLRVLDGAVAVFDATAGVEPQSETVWRQADKYNVPRIAFMNKMDKTGADFEMAVQTMVERLGAHPIPVQLPMGAESDFAGVIDLIEMKAIRWLNPEGTEMVVEEIPTQWKDKADEAREDMIEKIAEVDDEIMEMYLEGEEPSIEQIHAALRRITIAGLGTPVFCGSAKMNLGIQPLLDGIVRYLPSPLDLPPVKGFDKAGNEIQVQPTENAPFVAYAFKIQTDPYVGKLTFLRVYSGKLEKGSYVINTTKGVKERVSRLIFLHADKREDVEYVRAGDIVGVIGMKSTITGDTVCEENTYVILEKMEFPEPVISIAIEPETKDDETKLSKALQSLLDEDPSLRAYVDQETGETILSGMGELHLEIIVDRLKREFNVNVRVGKPQVAYRETITKDVKIEGKYIRQSGGRGQYGHVVVQFEPLGLDKTFEFVDKTVGGVIPKQYIPAIEEGIREAMQVGVLAGYPVVGIKATLLDGSYHEVDSSEMAFKIAASMAFKEAMEKGNPVLLEPIMKVEVTTPEDYMGNIIADLNSRRAHIDALENRGHLRVVKALVPLSEMFGYATTLRSLSQGRANYTMVLSHYEKVPEKVAEKILKG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation; During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively; Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome..2. The designed protein should have a high specificity for GTP binding, with minimal off-target effects.3. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining GTPase activity, translation elongation factor activity, GTP binding.4. The designed protein must have at least one tr-type G domain. The designed protein sequence is ",
    "response#1": "FRNIGISAHIDSGKTTLSERILFYCDRIHAIHEVRGKDGVGATMDNMELERERGITIQSASTQVQWKGHTINVIDTPGHVDFTIEVERSLRVLDGAVLVLCSVAGVQSQSITVDRQLRRYHVPRISFINKCDRTGANPFKVCAQLREKLSLNAHLMQLPIGLEDRLEGVIDLISLKALYFEGESGAHVREAPIPEQYQADVKKYRDELIDAASLFSDELAEAYLEGTETDQLIRAAVRAGTIAEKFVPVFCGSAYKNKGIQPLLDAITYYLPAPVDVPQIEVTFDIDANGIVNVSAKDLGTSKEQHIVIKANSGLSDDEIEKMVRDAELHAEEDKKRREEAETRNNAEALAFQAEKNLTEYRETIRRSVEQQGKYIRQTGGRGQYGDVWLRIEPREPGAGFEFENAIVGGVVPREYIPAVEKGVREQMENGIRAGYPVVDVKVTIFEGSYHDVDSSEMAFKIAGSMAFKEGASKEDVEKKIEDVVKELQKYRETIRASVEQEGKFVRQSGGRGQYGHVLLRIEPLTAEDKGEDEDMTFKFASEIVGGVVPKEYVPAVEKGAYEQLQNGVIAGYPMIDVKVTLFDGSYHDVDSNETAFKVASSMAIKEGAPKAGDPVLLEPVMAVEVTTPEEYMGDVIGDLNSRRGQIQSMEERAGTRVVKALVPLSEMFGYVGDLRSRTQGRANYTMVFHSYAEVPSNVSQEIVEAKK",
    "response#2": "KFLRNIGIMAHIDAGKTTTTERILYYTGLTHKIGEVHEGGAVMDWMEQEQERGITITAAATTTFWKYPTVQGQVTKDTQQYSINIIDTPGHVDFTVEVERSLRVLDGAVALFCAVSGVEPQSETVWRQADKYRVPRICFVNKMDRAGADFFNAVSEIKSKLGANPIPLQIPIGSEDTFKGVVDLIRNQAIIWNDEDLGMTYQVVPIPEDLKETVEEYRQKLMESVAEYDEVLLEKYFNDPNTITTEEIMVAIRKAVIDMSISPVLCGASFKNKGVQALLDAVCTYLPSPQDVPGIEGLDTYRETIRASVEQEGKFVRQSGGRGQYGHVLLRIEPLTAEDKGEDEDMTFKFASEIVGGVVPKEYVPAVEKGAYEQLQNGVIAGYPMIDVKVTLFDGSYHDVDSNETAFKVASSMAIKEGAPKANPVLLEPVMAVEVTTPEEYMGDVIGDLNSRRGQIQSMEERAGTRVVKALVPLSEMFGYVGDLRSRTQGRANYTMVFHSYAEVPSNVSQEIVKTRNIGIMAHIDAGKTTTTERILYYTGVTHKIGEVHEGAATMDWMEQEQERGITITSAATTCFWGDHRVNIIDTPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEPQSETVWRQADKYRVPRIAFINKMDRVGADFFRGVGMIRDRLKANPVPIQLPIGAEDTYRGVVDLVKKVRNIGIMAHIDAGKTTTTERILFYTGINRKVGETHDGASTMDWMEQEKERGITITSAATTCFWKDNQINIIDTPGHVDFTIEVERSLRVLDGAVAVFDAKEGVEPQSEQVWRQATKYDVPRICFVNKMDKLGADFYYTVQTIVDRLGAKPLVMALPIGAEDDFDGIVDLLNMRAVTWRGKVEIGAEATYEEIPEDLKDKAEEYREKLVETVAESDEELMERYFAGEEITVDELKAQIRKLTISSEVYPVYCGSAYKNKGIQPMLDAVIDFL",
    "response#3": "RTRNIGIMAHIDAGKTTTTERVLYYTGVSHKMGEVHEGSAVMDWMEQEQERGITITSAATTCYWLGMDQQYPKHRINIIDTPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEPQSETVWRQANRYHVPRLGFVNKMDRAGANFLRVVNQVKDRLNANPIPIQLPIGAEEDFKGVIDLIREKAIYWNEADRGRTYELADIPEDMKAEVQKWREKMIEAAAESSEELMDKYLEAGDLSPEQIRQGLRQRTLANEIVPILCGSAFKNKGVQALLDAVIDYLPTPAERSATDILEPMMAVEVITPEDYMGDVIGDLNSRRGQIQAMEERSGARVVKALVPLSEMFGYIGDLRSKTQGRANYSMVFDSYAEVPANVSVIMEPIMAVEVTTPDEFTGPVTGDLNKRRGIMRGMDAKGNTQVVKADVPLSELFGYVTDLRTITSGRAAATLTFSRYEQVPKQLADSAILEPLMKVEVEVPEDYMGDVIGDLNRRRGQVTNMGDRSGNKIVDAFVPLAEMFGYSTDLRSATQGRATYSMEFDHYEEVPKNVSEEIVEPIMAVEVTTPDEFTGPVTGDLNKRRGIMRGMDAKGNTQVVKADVPLSELFGYVTDLRTITSGRAAATLTFSRYEQVTEILEPIMKVSIEGPQEFQGNIFGLINQRRGIIISSTEDDNFTRVDAEVPLSEMFGFSTILRSSTQGKAEYSMEFAKYGKAPASISEVLLEPVMKVEVVTPEEFMGDVMGDLNRRRGLVQGMDDSPSGKIIRATVPLAEMFGYATDLRSQTQGRASYTMEFADYEEAPSSVVSHLLEPMMAVVVETPEDYMGNVMGDLSGRRGIVQGMDDLPGGMKEIKAEVPLAEMFGYATQLRSLTQGRATYSMEFKHYSEAPKSVA"
  },
  {
    "reference": "MNIAVVGLSHRTAPVEVREKLSISDEHIEKSFCSLNATEQVLEVSILSTCNRLEIYALVKNQELGVSAIKEFLIDHSGLSKEDLFPHLFTFNQAEAVNHLMRVSGGLDSLVLGEGQILSQVKKMVRLGQDYKSIGPILNRLLTQAVSTGKKVRSDTNLGTGAVSISSAAVELAQLKLGQSLGKDQLMTLQSEKVAVIGAGRMSRLLIQHLQSKGCSKLTLLNRTLNRAESLAKDFPDLDVKCGLLDDLDKCIEFSTLIFTSTASNTPIINSELLSGFKRNNNLLRLIDIGVPRNIASDVSGLPGFEAYDVDDLQEVVARNQDARQQIAIEAQNLIDEEARVFLEWWASLEAVPTINRLRSNLESIRKEELQKALSRMGPDFSARERKVVEALSKGIINKILHTPVTNLRAPQPSSQRKESLKIVESLFELGVSENDQ",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be optimized for glutamyl-tRNA reductase activity.2. The designed protein should have a high specificity for NADP(+), substrate binding, with minimal off-target effects.3. Ideal protein should have a binding affinity for key enzymes in the chlorophyll biosynthesis pathway to enhance the overall efficiency of the pathway.4. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA)..5. The designed protein must have a Nucleophile active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "TGRIFERVSDQHVLFIGAGEMIELCAAHFAGAGPRSMTVANRTEARAEALAARLGAQTMRLDAIADALPRFDVVVSCTASPLPIVGLGMVERAVKVRRHRPIVMVDLAVPRDVEPEVGQLDDVFLYTVDDLAQTGRIFERVSDQHVLFIGAGEMIELCAAHFAGAGPRSMTVANRTEARAEALAARLGAQTMRLDAIADALPRFDVVVSCTASPLPIVGLGMVERAVKVRRHRPIVMVDLAVPRDVEPEVGQLDDVFLYTVDDLAQSWTGDVRALVTTEVAGFLDRRRAERVAPTVVALRAHAAAVVRDELERLRTRLPDLDNREWDMVSGTVRRVVDKLLHAPTVRVQQLAEAPGGDSYAEALRELFDPAR",
    "response#2": "QLMTLESELVAVVGAGRMSRLLLQHLQAKGCSGVMLLNRTRQRAEDLSADFPELPVECRPLDDLNHCLSTCSLVFTSTAADDPIVDASLLKQLKRRSFLRLIDIGVPRNIASDVVDVPGVESHDVDDLHETRFLTGYEAEGILEEELETFLAWWHALETVPIIRSLRQKMEAIRTQELEKALSRLGSEFADKHQGVIEAMTRTIINKILHDPTVQLQSQRDLESRQRAMQTLQDLFNL",
    "response#3": "TGRIFERVSDQHVLFIGAGEMIELCAAHFAGAGPRSMTVANRTEARAEALAARLGAQTMRLDAIADALPRFDVVVSCTASPLPIVGLGMVERAVKVRRHRPIVMVDLAVPRDVEPEVGQLDDVFLYTVDDLAQEAEVVLQEETRLFLEWWDSLEAVPTINRLRATLEAIRAEELQKALSRMGPDFSARERKVVEALSKGIINKILHTPVTQLRAPQARPERQQALRVVASLFES"
  },
  {
    "reference": "MGNVPSAVKHCLSYQQLLREHLWIGDSVAGALDPAQTSLLTNLHCFQPDVSGFSVSLAGTVACIHWETSQLSGLPEFVKIVEVGPRDGLQNEKVIVPTDIKIEFINRLSQTGLSVIEVTSFVSSRWVPQMADHTEVMKGIHQYPGVRYPVLTPNLQGFHHAVAAGATEISVFGAASESFSKKNINCSIEESMGKFEEVVKSARHMNIPARGYVSCALGCPYEGSITPQKVTEVSKRLYGMGCYEISLGDTIGVGTPGSMKRMLESVMKEIPPGALAVHCHDTYGQALANILTALQMGINVVDSAVSGLGGCPYAKGASGNVATEDLIYMLNGLGLNTGVNLYKVMEAGDFICKAVNKTTNSKVAQASFNA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that can modulate the overall flux of metabolites through the (S)-3-hydroxy-3- methylglutaryl-CoA degradation, Metabolic intermediate metabolism pathway, enhancing or suppressing its activity as needed.2. The protein should have a high affinity for a divalent metal cation.3. A key design requirement for the protein is the optimization of the active site to achieve high catalytic efficiency for (3S)-hydroxy-3-methylglutaryl-CoA = acetoacetate + acetyl-CoA.4. The designed protein must have at least one Pyruvate carboxyltransferase domain.5. The designed protein should be expressed specifically in the membrane, cytosol The designed protein sequence is ",
    "response#1": "MKALVNQLHERAQYVRLGGSEKARARHTSRGKLLPRDRIDNLIDPGSPFLEFSQFAGYKLYGEEEVPAGGIITGIGRVSGVECMIVANDATVKGGTYYPVTVKKHVRAQEIALQNRLPCIYLVDSGGANLPRQADTFPDRDHFGRIFYNQAIMSSKNITQIAVVMGSCTAGGAYVPAMADENIIVQRQGTIFLAGPPLVKAATGEEVSAEDLGGADLHCRRSGVTDHYALDDHHALHLTRKVVRSLNYQKKLDVTVEDKAGFYVNRILSPYINEAGYCLVEGAKRWDPEITSERVHRRFNSDYLNVPMGWVLRSYDLNRSNEQVQAQISIGDDRQPVTLLSGRGDGAMSALVDALNRRIGGEVKVVSFDEYSLGDNTEANAMACVRVQVGDSTQSAVAMAVDTTAAALQAILSAVGKY",
    "response#2": "HTSRGKLLPRDRIDNLIDPGSPFLEFSQFAGYKLYGEEEVPAGGIITGIGRVSGVECMIVANDATVKGGTYYPVTVKKHVRAQEIALQNRLPCIYLVDSGGANLPRQADTFPDRDHFGRIFYNQAIMSSKNITQIAVVMGSCTAGGAYVPAMADENIIVQRQGTIFLAGPPLVKAATGEEVSAEDLGGADLHCRRSGVTDHYALDDHHALHLTRKVVRSLNYQKKLDVTVEPSEEPLFPADELYGIVGANLKRSFDVREVIARIVDGSRFNEFKALYGDTLVTGFARIFGYPVGIIGNNGVLFSESAKKGAHFVQLCCQRNIPLLFLQNITGFMVGKDYEAEGIAKDGAKMVAAVSCAKVPKITVIIGGSYGAGNYGMCGRAYSPRFLYMWPNARISVMGGEQAATVLATVARDQRAREGKQFSSAEEAALKEPIIKRFEEEGNPYYSSARLWDDGIIDPVDTRLVLGLSISAALNAPIV",
    "response#3": "EPLFPADELYGIVGANLKRSFDVREVIARIVDGSRFNEFKALYGDTLVTGFARIFGYPVGIIGNNGVLFSESAKKGAHFVQLCCQRNIPLLFLQNITGFMVGKDYEAEGIAKDGAKMVAAVSCAKVPKITVIIGGSYGAGNYGMCGRAYSPRFLYMWPNARISVMGGEQAATVLATVARDQRAREGKQFSSAEEAALKEPIIKRFEEEGNPYYSSARLWDDGIIDPVDTRLVLGLSISAALNAPIQS"
  },
  {
    "reference": "MVLAFQLVSFTYIWIILKPNVCAASNIKMTHQRCSSSMKQTCKQETRMKKDDSTKARPQKYEQLLHIEDNDFAMRPGFGGSPVPVGIDVHVESIDSISETNMDFTMTFYLRHYWKDERLSFPSTANKSMTFDHRLTRKIWVPDIFFVHSKRSFIHDTTMENIMLRVHPDGNVLLSLRITVSAMCFMDFSRFPLDTQNCSLELESYAYNEDDLMLYWKHGNKSLNTEEHMSLSQFFIEDFSASSGLAFYSSTGWYNRLFINFVLRRHVFFFVLQTYFPAILMVMLSWVSFWIDRRAVPARVSLGITTVLTMSTIITAVSASMPQVSYLKAVDVYLWVSSLFVFLSVIEYAAVNYLTTVEERKQFKKTGKISRMYNIDAVQAMAFDGCYHDSEIDMDQTSLSLNSEDFMRRKSICSPSTDSSRIKRRKSLGGHVGRIILENNHVIDTYSRILFPIVYILFNLFYWGVYV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel..2. The designed protein should contain Extracellular, Cytoplasmic topological domains.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.4. Design the protein with a signal peptide incorporated.5. The ideal protein would be one that plays a critical role in nervous system process, chloride transmembrane transport, regulation of membrane potential through excitatory extracellular ligand-gated ion channel activity, GABA-A receptor activity, transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential, neurotransmitter receptor activity within synapse, plasma membrane, neuron projection, chloride channel complex. The designed protein sequence is ",
    "response#1": "ILNSLLRDYDSKLRPDIGVRPTVIETDVYVNSIGPVDPINMEYSIDIIFGQTWFDRRLRFPGSSKVLMLNSNVVGKIWVPDTFFRNARKSDAHWVTTPNRLLRIRGDGRVLYTLRLTIAAECYLQLRNFPMDEHSCPLEFSSYGYPQNEIEYKWKQTSVEVADPKYWRLNQFAFVGLRNSTEIWRTQSGDYVVMTIFFDLSRRR",
    "response#2": "TRILDRLLDGYDNRLRPGLGDAVTEVKTDIYVTSFGPVSDTDMEYTIDVFFRQTWHDERLKFDGPMKILPLNNLLASKIWTPDTFFHNGKKSVAHNMTTPNKLLRLVDNGTLLYTMRLTIHAECPMHLEDFPMDVHACPLKFGSYAYTTAEVVYSWTLGKNKSVEVAQDGSRLNQYDLLGHVVGTGGXC",
    "response#3": "VLPAVELAIEQIRNESLLRPYFLDLRLYDTECDNAKGLKAFYDAIKYGPNHLMVFGGVCPSVTSIIAESLQGWNLVQLSFAATTPVLADKKKYPYFFRTVPSDNAVNPAILKLLKHFRWRRVGTLTQDVQRFSEVRNDLTGVLYGEDIEISDTESFSNDPCTSVKKLKGNDVRIILGQFDQNMAAKVFCCAFEESMFGSKYQWIIPGWYEPAWWEQVHVEANSSRCLRRSLLAAMEGYIGVDFEPLSSKQIKTISGKTPQQFEREYNSKRSGVGPSKFHGYAYDGIWVIAKTLQRAMETLHASSRHQRIQDFNYTDHTLGKIILNAMNETNFFGVTGQVVFRNGERMGTCGVIGIAQVLPAVELAIEQIRNESLLRPYFLDLRLYDTECDNAKGLKAFYDAIKYGPNHLMVFGGVCPSVTSIIAESLQGWNLVQLSFAATTPVLADKKKYPYFFRTVPSDNAVNPAILKLLKHFRWRRVGTLTQDVQRFSEVRNDLTGVLYGEDIEISDTESFSNDPCTSVKKLKGNDVRIILGQFDQNMAAKVFCCAFEESMFGSKYQWIIPGWYEPAWWEQVHVEANSSRCLRRSLLAAMEGYIGVDFEPLSSKQIKTISGKTPQQFEREYNSKRSGVGPSKFHGYAYDGIWVIAKTLQRAMETLHASSRHQRIQDFNYTDHTLGKIILNAMNETNFFGVTGQVVFRNGERMGTCGVIGIAQ"
  },
  {
    "reference": "MSALTNLKDLSLEELTAFLIELGEKPFRAKQIADWVFKKGVAEIADMTNLPLSLRERLSKTAYIGRLEIMKEQQSRDGTTKYLFELADGNTVETVFLKHNYGNSVCVSTQVGCKMGCLFCASTIGGFYRNLSPGEIYDQVLRIEQDKKKRVSSVVIMGSGEPLDNYEAVLKFIRLITAPYALNVGMRHITLSTCGLVPQIYKLAEEKLALTLSVSLHAPNNELRNKLMPVNRKYPLEELIPACHEYIKKTGRRITFEYTLIKDVNDSQGHAEELARLIKGMLCHVNLIPVNPVAERRWYRPSTETVKRFQTILERNRVPATVRREMGTDIDAACGQLRRKYNLALETGGKKDGFLD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have a binding site that is specific to the target [4Fe-4S] cluster, S-adenosyl-L-methionine ligand.2. A protein that contributes to rRNA base methylation by functioning in cytoplasm with metal ion binding.3. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity.4. The protein requires [4Fe-4S] cluster to activate a critical catalytic site.5. The S-methylcysteine intermediate active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "MTACISSQVGCSLTCAFCATGKLKRMRNLEASEIYDQVVTLKNEAENTYGIPLSNIVYMGMGEPLLNYRSTLESVSKITSPEGLGMSPQRITVSTAGIAKMIMKLGDDGVKFNLALSLHAANDEKRNKIMPINEQNTLDALAEALRYFYDKTGTRVTYEYIIFKEFNDSLQDARELAEFCKHVPCKVNIIEYNPIDDGEYRQADSEKVDAFARYLAAG",
    "response#2": "MTACVSSQVGCSLSCKFCATGYMERKRNLNPDEIYDQVVLIAKQANDNYQMPLSNIVYMGMGEPLLNYSNVMKSIERITAEDGLNMAAKRITVSTAGIAKMIKKLGDDQVRFNLALSLHAANDEKRNTIMPINEQNSLKALAEALKYYFAKTKNPVTYEYIVFDNFNDGIEDAIELAKFCRHLPCKVNIIEYNPISFAAYQNAETDKIDAFADYLR",
    "response#3": "MTACISSQVGCSLTCAFCATGKLKRMRNLEASEIYDQVVTLKNEAENTYGIPLSNIVYMGMGEPLLNYRSTLESVSKITSPEGLGMSPQRITVSTAGIAKMIMKLGDDGVKFNLALSLHAANDEKRNKIMPINEQNTLDALAEALRYFYDKTGTRVTYEYIIFKEFNDSLQDARELAEFCKHVPCKVNIIEYNPIDDGEYRQADSEKVDAFARYLE"
  },
  {
    "reference": "MFAPLLKKLFGSKNEREVKRMLKAVQSVNALEEQMLSLSDEQLRSKTEEFKARLEKGETLDQILPEAFAVCREAGKRVMGMRHFDVQLIGGMTLHEGRIAEMRTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLARRDANWMRPLYEFLGLSVGIVTPFQPPEEKRAAYASDITYGTNNEFGFDYLRDNMAFSLQEKNQRELNFAVIDEVDSILIDEARTPLIISGQAEDSSKLYQQINLLIPRLTQHIEEEEGVVTQEGHFSIDEKTRQVELNEQGHQYIEELLTQAGLLAEGESLYSAHNLGLLTHVYSGLRAHKLFHRNVEYIVQNNQVLLIDEHTGRTMPGRRLSEGLHQAIEAKEGLQIQPESQTLASTTFQNYFRLYKKLAGMTGTADTEAFEFQQIYSLPVVVIPTNKPLARKDFNDLVYLTQEEKFAAIIADIKECRNQGRPVLVGTATIESSEYVSRLLEAEGFEHKVLNAKHHDKEAEIIAQAGRPGAVTIATNMAGRGTDILLGGNWEVEVAALENATEEQIAQIKADWQKRHQQVLEAGGLHVIASERHESRRIDNQLRGRAGRQGDPGSSRFYLSLEDSLMRIFASDRVKNFMKALGMESGEAIEHRMVTNAIEKAQRKVEGRNFDMRKQLLEYDDVANEQRKVIYHMRNSLLAADEIGQTIAEFRQEALDAAISAHIPPQSLPEQWDIPGLEAVLYSDFGTRLPIQQWLDEDEKLYEETLREKILQALLDAYNEKEDMAGAEALRSFEKQIVL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Interacts with the SecYEG preprotein conducting channel2. The desired protein should be able to perform ATP + H2O + cellular proteinSide 1 = ADP + phosphate +   cellular proteinSide 2.3. The ATP binding site should be located in a region of the protein that is accessible to the ligand.4. The ideal protein would be one that plays a critical role in protein import, protein targeting through metal ion binding, ATP binding within plasma membrane, cytoplasm.5. The presence of Zn(2+) is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "MESKLLTKVIASAQAKVEAHNFEGRKNLLQYDDVANEQRKAIYEQRNYLLETNDISAMIETIRDDVFNHVISRYIPPQSIEEMWDIAGLEEVLHHQFGMELPIQQWLEKEKDLHEETLRERIINLAKQEYQSKEEKVGAEVMRNFEKGVMV",
    "response#2": "PLESKMVTRAIRSAQTQVEQQNFEIRKNVLKYDEVLNKQRAVIYAERRRVLHGDDLHEQVGHMIDDVIRDYVRAATEEGYAEDWDLEQLWTALRSLYPVGLTIDQVVAECGGDRSGLTAEFLIERLTEDAHRAYAEREAAA",
    "response#3": "PVDMNQKWYTDFAKLAERLQRGENGEGDYEVDEKKRTISITERGVQRVEDWLGIDNLYEPTNTPLVGYLHNALRAKELYKRDRDYVVIDGEVLIVDEFTGRILYGRRYNEGMHQAICD"
  },
  {
    "reference": "MNQKDTRKETLEFNKLQKRLRRNVGNAITDYNMIEEGDVVMACISGGKDSFAMLDILLNLQKAAPIKFEVVAVNLDQKQPGFPEHILPEYFETLNIPYYIVDKDTYSVVKEKVPEGKTTCGLCSRLRRGTLYSFAEKIGATKLALGHHMDDIVETMFLNMFHGSRLKAMPPKLRSDDGRNVVIRPLTYCREKDLIKYAEHKDFPIIPCNLCGSQENLQRQAIKAMLIDWDKKTPGRVEAIFKSIQNVSPSQLADRELFDFVNLPLDRDGSREEYEFSEAVVSSTNIDESLFIDVTNI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that The sulfur atoms are provided by the cysteine/cysteine desulfurase (IscS) system. The designed protein sequence is ",
    "response#1": "DTNKLLRNFSRCRNWEERYLYMIELGAKLPPLTDEQRQPENLIAGCQSQVWILLRMNHQNKVEFIGDSDAAIVKGLVAIVFILFQGKTTQEILDLDVTEYFGKLSLEQHLTPSRTQGLHAMIHAIRNRTSKC",
    "response#2": "DTNKLLRNFSRCRNWEERYLYMIELGAKLPPLTDEQRQPENLIAGCQSQVWILLRMNHQNKVEFIGDSDAAIVKGLVAIVFILFQGKTTQEILDLDVTEYFGKLSLEQHLTPSRTQGLHAMIHAIRNRTSKC",
    "response#3": "DTNKLLRNFSRCRNWEERYLYMIELGAKLPPLTDEQRQPENLIAGCQSQVWILLRMNHQNKVEFIGDSDAAIVKGLVAIVFILFQGKTTQEILDLDVTEYFGKLSLEQHLTPSRTQGLHAMIHAIRNRTSKC"
  },
  {
    "reference": "MGIKALGADGLAALVAELGQPRYRADQLLHWLYGRNAASYAEMTDLPAGFRENLGSRLALVPARVLERQVSADGTRKYLIGLADGVTVETVGLPTEDRLTVCFSTQAGCAMGCSFCATGEGGLVRDLGPGEMVDQLAVVAADFSARVTNAVAMGQGEPFANYDATLAALRLMNHPKGLGIGARHLTVSTCGLIAGIDRFATEPEQFTLAVSLHSAVQATRDRLMPGVRGLPLPRLREALANYAAATGRRPSVEYALVAGVNDSDRELEALRAFCSGLLIHVNLIPVNPVASAGFERPEAPALARFEETLQAAGIEVSTRTERGSDIDAACGQLSQRHRP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a fast turnover rate for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.2. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.3. The presence of Radical SAM core domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "KPPVHLADIAPADRKAHVAELGFPGYRAAQLATHYFVHHTNDPAEMTDLPAASRDALAGAVLPKLLTEVRRFTTDNGDTIKFLWKLHDGALVESVLMRYPGRVTLCVSSQAGCGMNCPFCATGQAGLTRNMSAAEIVEQVVMANAALARGELGGRKKGDHTPDRVSNIVFMGMGEPLANYARLMSAVRTMLAPAPDGLGMSARGVTVSTVGLVPAIRKLADEDLPVTFALSLHAPDDQLRDELIPVNSRWKVDEALDAARAYFDKTGRRVSIEYALIKDMNDHGWRADLLAEKLNERGRGWVHVNPIPLNPTPGSIWTASDTDVQQEFVRRLEAAGIPTTIRDTRGKEIDGACGQLAAL",
    "response#2": "EESRATLCISSQVGCALNCSFCSTAAQGFNRNLSSAEIVGQLWTVSRLLETDGMRAVSNVVFMGMGEPLLNLDQVLPATRVMQDDLAYGLSKRRVTLSTAGVVPGLARLAGESDVALALSLHAPDDALRSQLVPLNIKYDIDSVIRACADYVGDGRRRVTVEYVMLDGVNDSDEHARALVRRLRHLPSKVNLIPFNPFPSTTYRCSPPGRIERFRDILYRAGIVTITRRTRGDDIMAACGQLKG",
    "response#3": "KPPVHLADIAPADRKAHVAELGFPGYRAAQLATHYFVHHTNDPAEMTDLPAASRDALAGAVLPKLLTEVRRFTTDNGDTIKFLWKLHDGALVESVLMRYPGRVTLCVSSQAGCGMNCPFCATGQAGLTRNMSAAEIVEQVVMANAALARGELGGRKKGDHTPDRVSNIVFMGMGEPLANYARLMSAVRTMLAPAPDGLGMSARGVTVSTVGLVPAIRKLADEDLPVTFALSLHAPDDQLRDELIPVNSRWKVDEALDAARAYFDKTGRRVSIEYALIKDMNDHGWRADLLAEKLNERGRGWVHVNPIPLNPTPGSIWTASDTDVQQEFVRRLEAAGIPTTIRDTRGKEIDGACGQLAAL"
  },
  {
    "reference": "MPKTDIARRVYNHTWKLDPIVRSLLDTDFYKLLMLQMIWQLYPDVDATFSLINRTKTVRLADEIDEQELRDQLDHARGLRFTKKEMIWLAGNSFYGRTQIFSPEFLAWLARFRLPEYELSRRDGQFELTFRGRWAETTMWEIPALAIINELRSRAAMKGLGPFTLDVLYARAKAKMWSKVEQLREHPNLRISDFGTRRRHSFLWQRWCVEALKEGIGSSFTGSSNVLLAMDNDLEAVGTNAHELPMVAAALTRNDRELAAAPYKVLQDWNQLYGGNLLIVLPDAFGTAAFLRDAPDWVADWTGFRPDSAPPIEGGEKIIAWWKKMGRDPREKLLIFSDGLDVDTIIRTYCHFEGRVRMSFGWGTNLTNDFAGCAPTEINGLNPISIVCKVSEANGRPAVKLSDNPRKATGDPAEVERYLKFFGSEDFVEQSVRV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be optimized for activity in NAD(+) biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "GILAGSATYPGAAVLCTGAAVAATSGMVRYAGSAGQQVLSQWPEVIAAPNASASGRVQAWAVGPGLGTDDRGATALWFALESGLPTIVDADGLTILAAHPDLVEGREAPTVLTPHAGEFARLTGSPPGDDRVGATRKLADRLGVTVLLKGNVTVIAEPGGPVYLNAAGQSWAATAGSGDVLTGVIGALLAAGLPAGEAAAAAAFVHARAANLAACDPGPSPAPTSASRIF",
    "response#2": "GFAEMLKYGIALHSGLWLRLKAMVAGGWLTPRRVRKTPDGWLLPIARCVQIKSELVSSDERDTQGLRALLNFGHTVGHAIERALNYRDWLHGEAIAAGMVAEAQIGVLMGITPEETLAELKHTLQQAGLPVALPPVAPETLLEAMRADKL",
    "response#3": "GILAGSATYPGAAVLCTGAAVAATSGMVRYAGSAGQQVLSQWPEVIAAPNASASGRVQAWAVGPGLGTDDRGATALWFALESGLPTIVDADGLTILAAHPDLVEGREAPTVLTPHAGEFARLTGSPPGDDRVGATRKLADRLGVTVLLKGNVTVIAEPGGPVYLNAAGQSWAATAGSGDVLTGVIGALLAAGLPAGEAAAAAAFVHARAANLAACDPGPSPAPTSASRIF"
  },
  {
    "reference": "MTQVFLASRNPKKLAEMQRILAEHMPDVAVLGLDDVTPYDEPAETEPTFEGNALIKARVAVAATGLPSLADDSGLCVDALNGMPGVLSARWAGTTGTSVASGAADAAGTTGRLGPTDRDRANNELLLEQITDVPDERRGAHFTCAVAFAYPAGAGGVVATVVHARMPGRVIREMRGEGGFGYDVLFVADGHDRTSAELSVEEKDAISHRGQALREIAPVVATVLTGL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein with metal ion binding, ribonucleoside triphosphate phosphatase activity functions is required to enable nucleotide metabolic process.2. The Mg(2+), substrate binding site should be stable and able to withstand changes in environmental conditions.3. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in dITP + H2O = dIMP + diphosphate + H(+) are properly positioned for optimal catalysis.4. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP The designed protein sequence is ",
    "response#1": "MRKVIVASNNAKKLAELRQILSPLDIELVGQSAFNIPEAEEPHVTFVENALAKARHASAHTGKPSVADDSGLIVDALGGAPGIYSARFAGENASDADNNAKLLAELAGVPEAQRSARFRCVIVLMRSADDPAPLIVEGRWHGVILEEPRGSGGFGYDPYFLLPELNRTAAELDPALKNRLSHRGQALRKLLAVLEKA",
    "response#2": "MNIILASNNKGKVREIKEILAPLQVKLLTPKDADIDVDIVEDGKTFEENSLIKARAVAHSTGLPAIADDSGLTVEALNGAPGVYSARYAGDHASDNDNLEKLVKELEGTPNRKAWFKTAVALVTPKGEHIIAEGICPGRISEEPRGDNGFGYDPLFIAEGMEKTFAELPAEKKNAVSHRGKALAKLLPMLKEALK",
    "response#3": "MMNEVLVVATHNQGKTREIADLLAPYGFAVKTQSEFGVPEAEETGTTFAENARLKAAAVARATGLPALADDSGLCVDALNGAPGVLSARWSGRHGDDAANTALLLAQLADVDPSHRGAHFVCAAALALPDGTEQVVQGVWPGTLLREARGANGFGYDPILQPDGLELSSAELSPAEKNAHSHRGRAFRALAPVLAERLG"
  },
  {
    "reference": "MTKFSEFGLDEKIVKSVNRMGFEEATPIQEKTIPLGLEGKDLIGQAQTGTGKTAAFGLPMIHKIDQKSNNVQALIIAPTRELAIQVSEELYKLSYDKHVRVLAVYGGSDISRQIRSLKKNPQIVVGTPGRILDHINRRTLKLDHVETLVLDEADEMLNMGFIDDIETILKEVPAERQTLLFSATMPDPIRRIGERFMHSPELIRIKAKEMTALLIEQFFVKVHEKEKFDVLSRLLDVQAPELAIVFGRTKRRVDELSRALDMRGYVAEGIHGDLTQAKRMSVLRKFKEGKIDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAGKEGMAITFVQPREMGYLRIVEETTKKRMQPLQAPTWDEAFAGQLRVATEKIQEAITEENLADYKTFANELLEKYDATDIAAAMLKMLAKEPDKTPVHITEERPLPSRGGGGYKGKNGKGGKGGGYRGGSGKGGSYRDRNNSGKGRRSGGGSGGGSGSGGGGNRDRRGGGEQRSGGNKGNYSQKSK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that can perform ATP hydrolysis activity, RNA strand annealing activity in cytosol to support RNA secondary structure unwinding, RNA catabolic process.2. We need a protein that can perform ATP + H2O = ADP + H(+) + phosphate with high efficiency.3. The designed protein must have at least one Helicase ATP-binding domain.4. The protein should be able to bind ATP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MENLLQNQIVIVSGDAKCGKSAQVPQWCAEYCLSIHYQHGGVICTQVHKQTVVQLALRVADEMDVNIGHEVGYVIPFENCCTNETILRYCTDDMLQREMMSNPFLGSYGVIILDDIHERSIATDVLLGLLKDVLLARPELKLIINSSPHLISKLNSYYGNVPVIEVL",
    "response#2": "NPFAQSPEAADALEALGINSHPYAITSHTHAAAKAIESDLYEVVSHYLPKENPVSFLFMKPAKLRFFHRGPTHGDHFLNAHVEPKDVPRYPQETIINRLADIPTQIAFMGDTLHFLSPSFLTALFAHSPRLQTLYATLVLPPEPSTSRSLYPQIYTLTYSEDGFMYIPGGHAGASYFHKYDQLEWLTVGHLTAPGCPTVTAQRLETKGANHLFIFQRGNYITPERRTFATNDQYVTVPHIFLPENYNCRTPISKTTMMQMFFYIKSGKEAQREGHLGQVQQLL",
    "response#3": "GAAPPIEEQLKVHNNPYASLNIQQQRIRLPIFKNRGHILYMCERYRTIIIVGETGCGKSTQVPQFLLEAGWAADGRQIVITQPRRVAVVTLATRVAEEKDCILGHDVGYTVRFDDVSDKDTKVKFMTDGLLLREILADPLLSKYSIIMIDEAHERSCNTDILLGLLRKIIQIRNDLRIIVSSATLDAELFKDFFEMNETGNSDKDTAGIISVEGRTHR"
  },
  {
    "reference": "MLSFEGKVSSGLQKAGQFMEKEVYKKQYLDKLGFIPYHGTLNIKLSNNITLNLDNLHDKLKRIHGNGSFGDVLFLEAYLSTIDEKITKKGAILFPVKTVYDTDTLEYVSSEKLRDTLNLKDGDKVIIKIEK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein with riboflavin kinase activity, nucleotide binding functions is required to enable riboflavin biosynthetic process, phosphorylation, FMN biosynthetic process.2. The FMN, Mg(2+), CDP binding site should be located in a region of the protein that is accessible to the ligand.3. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.4. The protein design should consider the endogenous proteins and enzymes present in the FMN from riboflavin (CTP route): step 1/1, Cofactor biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "IVSGLGVGAKYVQLYRGVFNKYLGIDPYPGTLNIDIGQDFFIYTKKLKAKIIPPPRKGLGCVLAYPGILMGIKIYVIKPCITNHGRNILEIISDKNLRKTLNLKDNDIVEIL",
    "response#2": "LLGRVFSISGRVIHGNKIGRTMNYPTANILLSKNFLLTNGVYAVKIKYCPNKYAIGISNIGIKPSFSNTQKNKLLEVYLFDIKIDLYGKYIEIFIYKKIRDEQFFPSKKELKNQISQDILIVQKYFA",
    "response#3": "RVATAHLPTQWGHFIAHAYEADIEANPYIALVMGDVTSEEPVLVRIHSGCLTGDALRSLRCDCGDQLDLAMRRIAEEGRGVLLYIQQEGRGIGLVNKLKAYVLQDHGADTVEANELLGFPADIRDYSLGAQVLEDLGLRRIRLMTNNPAKFAALEGYDLEIVERVS"
  },
  {
    "reference": "MRVFVILPAAGLGTRMAAGSHTPHQPKQFLELEGVPVLIHTLRAFAAVPAVSAMIVAVRPNEIERVQAQVNEYGFQDKVRVVAGGDSRQQSVSRALATVECDASDIVLVHDAVRPLIEPAVIARTIEAVEKSGAAIVGLPAVDTIKQVERTAAGAIITATIPREYIVQAQTPQGFRCELLRRAFAEAEADGFTGTDEASLVERAGAQVTVVPGSPSNMKITQPGDLELAAFYLRQRSSR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be optimized for activity in isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 2/6 pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MRVGLGYDVHKLGYNTPLILGGVNIPFNKGLIGHSDADVLVHAIMDALLGALSLGDIGKHFPPSDMKYKNISSLKLLNYVSNLIKSKGYSIGNIDSTIIAEEPKLFPYIPDMRLNIAKILKVNTSIISVKATTEEGLGFTGKKEGIAAQSICLLF",
    "response#2": "MNPPSMLFPIQHVLTTPKRCPAPHKGMDFSIKQILEFFPIDEERFPSIALARQVLQDKGSSGPFFNAANEILVQRFLKKEIAWCDILDKLTRLMKNHRVSSCTSLDDVFSVDKEARS",
    "response#3": "MNPPSMLFPIQHVLTTPKRCPAPHKGMDFSIKQILEFFPIDEERFPSIALARQVLQDKGSSGPFFNAANEILVQRFLKKEIAWCDILDKLTRLMKNHRVSSCTSLDDVFSVDKEARR"
  },
  {
    "reference": "MFMSSDANACEIDIKPRSRVVTHGIEATTSRGMLRAVGMGDADWEKPQIGIASSWNNITPCNLSLDRLAQGAREGVHAAGGYPLQFCTISVSDGISMGHEGMHFSLVSREVITDSVETVLMAEALDGVVLLAGCDKSLPGMLMAAARLDVSAVFLYAGSIAPGYVTLKCGESKEVTIIDSFEAVGAYKAGLIDQDDLGRIERAICPGEGACGGMYTANTMASVAEALGMSLLGSASPPSADRRRDVYAHKSGEAVVELLKRGITARDILTKEAFENAIAVVMALGGSTNAVLHLLAIAHEAHVPLTIDDFNKIGNRVPHIADLKPFGRYVMNDVDRVGGIPVVINALMREGFIHGDVITVSGRTMAEEISDINPGLPDGKVIHSFSSPLHPTGGIKVLKGTLAPDGAVAKTAGFDTVVFQGPAMVFDRERAAMDALSAGNIKKGSVIVIRYEGPKGGPGMREMLAITAAIKGSGLGKDVLLLTDGRFSGGTTGLCIGHIAPEAVDLGPIAFVQDGDIIRVDIEKSGIDVLVDEKQLRARHLTPPPPRYTSGVLSKYSKLVKSASLGAIT",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit 2 iron, 2 sulfur cluster binding.2. The desired protein should be able to perform (2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-   oxopentanoate + H2O3. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "VIYSVDNPVKPDGGFRILYGDLAPEGAALKLSGHSRTAFSGPARVFESEEAAFAEIEANKIKAGDVVIIRNEGPKGGPGMREMLGVTAALVGQGLSEDVALITDGRFSGASKGFVIGHVSPEAAAGGPIGLIEEGDMIRIDVAARRIDVEADLSARRANWVRPEPKSVGGVFAKYAALVSSASRGR",
    "response#2": "VIYSVDNPVKPDGGFRILYGDLAPEGAALKLSGHSRTAFSGPARVFESEEAAFAEIEANKIKAGDVVIIRNEGPKGGPGMREMLGVTAALVGQGLSEDVALITDGRFSGASKGFVIGHVSPEAAAGGPIGLIEEGDMIRIDVAARRIDVEADLSARRANWVRPEPKSVGGVFAKYAALVSSASRGR",
    "response#3": "LHTVDDPIHERGAIRILTGNLAPDGAVIKITGEDHLHHEGPVRVFEEEKDAMAYVQEGHVDAGDVLCIRNEGPRGGPGMREMLGVTSAVAGQGHAEDVALFTDGRFSGATRGFSIGHVAPEAYVGGPIAALEDGDTVTIDIDALELSVDLTDEEIEARLEEYDPEPAYDSGVLAKYGRAFDSAANGD"
  },
  {
    "reference": "MPKYIFVTGGVVSSVGKGITTASIGRLLKGRGLSVAIMKLDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTDENLSRSSNVTTGQVYSAVIAKERRGDFLGGTVQVIPHITNEIKDRIRLVAEERQPDVVVVEVGGTVGDIEGQPFLEAIRQMRKEEGRRNVLYIHVTLLPYVGSTGELKTKPTQHSVKELRAIGIQPDVIVCRADKPVPQDVQAKIALFCDVDEDAVIPLPTAETIYEVPLMLEEAGLGRYIAEHFGWSDAPPDLNEWANMVHRLKHPRRRVEIALVGKYVELHDAYMSVVEALTHAGLAHDVAVDVIWVNSDLSSPESIAQQLRRAFGIVVPGGFGARGVEGKLAAVRFAREHKIPYLGLCYGLHMAVIEVSRNVLGLERANTTEIDPATPDPVVGLMPDQRGVDLGGTMRLGLYPCRLVPGTRVAEAYGQDVVDERHRHRWEINNDYLPRLEEAGLIVAGTSPDGRLVEVMELRDHPWFVGVQFHPEFKSRPTRPHPLFEGFVGAALEALREGDQRPLPLREIEAAPTTAND",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in 'de novo' CTP biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.2. The designed protein must have a Nucleophile; for glutamine hydrolysis active site that is highly conserved among related enzymes.3. The designed protein should contain one or more CTP synthase N-terminal domains that are essential for its function.4. The designed protein should have a high specificity for CTP, Mg(2+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "VNIAVVGKYVGLQDAYKSLREALVHGGIANKVKVNIEWIDSEIFESEDAVLHLEGVNGILVPGAFGKRGAEGKIKSITFARERLVPYFGICFGMQMAVVESARNLAGLPDASSTEFEKPCLPLVGLMTAWEHDGGQVTRSASSDMGGTLRLGAYDAHLTEGSQVQKIYGATTISERHRHRYEVNINYVEKLEETGMVFSGMSPDGVLPEIVEIPEHPWFIGVQFHPELKSRPLDPHPLFVSFG",
    "response#2": "IDIGLVGKYVELPDAYISVVESLKHAGYAYDSDIRVHWINSEKLDKDTIKDELSKVDGIIVPGGFGDRGIEGKIAAIQYARENNVPFLGICLGMQLATVEFARNVLGLDGAHSAEIDPSTPYPIIDLLPEQKDVEDLGGTLRLGIYPCKLKDGTKTKAAYNNADVVEERHRHRYEFNNEYRDQMEANGFVFSGTSPDGRLVETIEIENHPWFVASQFHPEFKSRPTRAQNLFLGFLKTL",
    "response#3": "VTVALVGKYVDLPDAYLSVTEALRAGGFHHRTRVSIRWVESDRCASGEGAAEQLRDVEAIVVPGGFGIRGVDGMVGALHHARRHGIPTLGLCLGMQAMVIEHARHELALATAHSTEFDPATEHPVVATMAEQEGILAGQGDLGGSMRLGSYPHTLVEGSLAARTYGTTEVAERHRHRYEVNNAYRERLEEAGLRLSGTSTLEDGRSLVEFVELDPAVHPFYLGTQAHPEFTSRPTRAHPLFAGLVGAAIK"
  },
  {
    "reference": "MGDTSPRTSVSTDGDTDHNNLMFDEGHLGIGASDSSDRSKSKMDQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTQLEQELQRARQQGVFISSSGDQAHSTAGDGAMAFDVEYRRWQEDKNRQMKELSSAIDSHATDSELRIIVDGVIAHYEELYRIKGNAAKSDVFHLLSGMWKTPAERCFLWLGGFRSSELLKLIASQLEPLTEQQSLDINNLQQSSQQAEDALSQGMDNLQQSLADTLSSGTLGSSSSGNVASYMGQMAMAMGKLGTLEGFIRQADNLRLQTYQQMVRLLTTRQSARALLAVHNYTLRLRALSSLWLARPRE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be optimized for nucleus-specific folding and stability, while maintaining DNA-binding transcription factor activity.2. Incorporate a signal peptide in the protein design.3. The protein must exhibit the following characteristics: May be involved in the induction of the systemic acquired resistance (SAR) via its interaction with NPR1; Binding to the as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. The designed protein sequence is ",
    "response#1": "IAAFEMEYTHWLEEQNRRVSEIRTALQAHIGDIELKMLVDSCLNHYANLFRMKADAAKADVFFLMSGMWRTSTERFFQWIGGFRPSELLNVVMPYVEPLTDQQLLEVRNLQQSSQQAEEALSQGLDKLQQGLVESIAIQIKVVESVNHGAPMASAMENLQALESFVNQADHLRQQTLQQMSKILTTRQAARGLLALGEYFHRLRALSSLWAAL",
    "response#2": "VSEIRTALQAHIGDIELKMLVDSCLNHYANLFRMKADAAKADVFFLMSGMWRTSTERFFQWIGGFRPSELLNVVL",
    "response#3": "KQATMAVECNNIPEQCKHSLKGRLCVEILEQEDKREQIPRDVPPSFAVAADELKMTLLDLENRVALAQRLFPTEAQAAMEIAEMKGTCEFIVTSLEPDRLTGTKRTSPGVKIAPFRILEEHQSRLKALSKTVELGKRFFPRCSAVLDQIMNCEDLTQLACGEDDTAEKRLQKKQRYMEIQETLKKAFSEDNLELGNSSLTDSTSSL"
  },
  {
    "reference": "MSVYLAEFLGTMLLIILGDGVVANVVLKKSKGHNSGWIVITTGWGLAVAMSVYLVGRISGAHINPAVTIGLAFIGQFPWSKVPGYIFSQILGAFVGAILVYLTYLPHWKETDDPDAKLAVFCTGPAVRKYGANLLTEIIGTMVLLMGVLGIGANKLADGLNPLLVGFLVWSIGLSLGGPTGYAINPARDFGPRLAHAILPIPGKRDSDWSYSWVPIIGPIIGGILGASLYNWLF",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be designed to catalyze the following reaction glycerol(in) = glycerol(out).2. The protein design must feature the NPA motif.3. The protein should have Helical domains to ensure proper insertion into the membrane.4. A protein that contributes to glycerol transmembrane transport by functioning in plasma membrane with glycerol channel activity.5. For general function, the protein need meet that Mediates glycerol diffusion across the cytoplasmic membrane via a pore-type mechanism. The designed protein sequence is ",
    "response#1": "KNTYGTGNFLVLNTGTELVRSQHGLLTTVAYQFGDNPPVYALEGSIAVTGSAVQWLRDQMKIIKTAAESEELARTVEDNGGMYFVPAFSGLFAPYWRSDARGAIVGLARYNDNAHLARATLEAICYQSRDVVVAMEQDSSVHLDVLRVDGGVTANDLCMQIQADILGVPVSRPVVAETTALGAAYAAGLL",
    "response#2": "KCTYGTGSFLVAHTGDRPIRSRHQLLTTVAWSDRTSTGYALEGALFTTGASVQWLRDGLGIIETADESEALAASVPDSGGVYFVPALSGLGAPHWDMGARGLLIGLTRGSGRGQIARAVLEAIAFQTREVTDALAADMGTPLTRLKVDGGAVRNNLLMQLQADVLGVPVERPQLIDTTAQGAAFAAC",
    "response#3": "KCTYGTGSFLVAHTGDRPIRSRHQLLTTVAWSDRTSTGYALEGALFTTGASVQWLRDGLGIIETADESEALAASVPDSGGVYFVPALSGLGAPHWDMGARGLLIGLTRGSGRGQIARAVLEAIAFQTREVTDALAADMGTPLTRLKVDGGAVRNNLLMQLQADVLGVPVERPQLIDTTAQGAAFAACG"
  },
  {
    "reference": "MVPPLALDIDGTLTTASGRLDARVFELLPDWDAPVVLATGKAFPYPVALAHFLGRAETVIAENGGVVHVDGETAILGDAEAPRAVVEAFRERGGDPGWGARDTVNRWRETEVALSLDADEALLREVAAAAGGDVEVVDTGYAYHVKSTGVSKGRGLERVGDALGIGPDEFVAIGDSENDVSTFAVAGESYAVANADGAAREAADIVVADSYMDGTAGVLADLRTRTE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The Nucleophile active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. For general function, the protein need meet that Catalyzes the dephosphorylation of 2-phosphoglycolate. The designed protein sequence is ",
    "response#1": "MDLLRFITCGSVDDGKSTLIGRLLWDSRQIFEDQLAVLKTDSKRHGTQGEQVDLALLVDGLAAEREQGITIDVAYRFFSTARRKFIVADTPGHEQYTRNMITGVSTADLAVILIDARLGILMQTRRHAYLVSLMGIRHIVLAINKMDLVDFDAKVSQSIEAEFNHLTQTLNFKSVTPIPISALTGDNITSRSSHMPWYLGPTLMELLETIDVTRPMDLLRFITCGSVDDGKSTLIGRLLWDSRQIFEDQLAVLKTDSKRHGTQGEQVDLALLVDGLAAEREQGITIDVAYRFFSTARRKFIVADTPGHEQYTRNMITGVSTADLAVILIDARLGILMQTRRHAYLVSLMGIRHIVLAINKMDLVDFDAKVSQSIEAEFNHLTQTLNFKSVTPIPISALTGDNITSRSSHMPWYLGPTLMELLETIDVTRPD",
    "response#2": "MDLLRFITCGSVDDGKSTLIGRLLWDSRQIFEDQLAVLKTDSKRHGTQGEQVDLALLVDGLAAEREQGITIDVAYRFFSTARRKFIVADTPGHEQYTRNMITGVSTADLAVILIDARLGILMQTRRHAYLVSLMGIRHIVLAINKMDLVDFDAKVSQSIEAEFNHLTQTLNFKSVTPIPISALTGDNITSRSSHMPWYLGPTLMELLETIDVTRPL",
    "response#3": "MDLLRFITCGSVDDGKSTLIGRLLWDSRQIFEDQLAVLKTDSKRHGTQGEQVDLALLVDGLAAEREQGITIDVAYRFFSTARRKFIVADTPGHEQYTRNMITGVSTADLAVILIDARLGILMQTRRHAYLVSLMGIRHIVLAINKMDLVDFDAKVSQSIEAEFNHLTQTLNFKSVTPIPISALTGDNITSRSSHMPWYLGPTLMELLETIDVTRPMDLLRFITCGSVDDGKSTLIGRLLWDSRQIFEDQLAVLKTDSKRHGTQGEQVDLALLVDGLAAEREQGITIDVAYRFFSTARRKFIVADTPGHEQYTRNMITGVSTADLAVILIDARLGILMQTRRHAYLVSLMGIRHIVLAINKMDLVDFDAKVSQSIEAEFNHLTQTLNFKSVTPIPISALTGDNITSRSSHMPWYLGPTLMELLETIDVTRPE"
  },
  {
    "reference": "MVFGQQLRNLDEKNRIALPPAFKNKLVEPLYLTIGFDGQADLRSEKEFEKFSAFLDQKNPFDAKIRQIKRQINSNTFEITLDKQGRITIPARIMQWIFAGEELGKEIYFVGAKDYVEIWSKSKFEALNEKVTPVGLEKLVEQAYNEK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein contains novel SpoVT-AbrB domains that confer a unique function or activity.2. The protein must have high specificity towards DNA binding. The designed protein sequence is ",
    "response#1": "TGTVKWFNEDKGFGFLTQDNGGADVFVHFRAIASEGFKTLDEGQKVTFEVEQGPKGLQASNVIAI",
    "response#2": "TGTVKWFNEDKGFGFLTQDNGGADVFVHFRAIASEGFKTLDEGQKVTFEVEQGPKGLQASNVIAS",
    "response#3": "MFLGRFTHAIDDKGRLAIPARFREAFRGQGVLTRGIDRCLTLYPMDSWQPLAEKVSSLSISDPDARAFRG"
  },
  {
    "reference": "MAHKKGQGSTQNNRDSAGRRLGVKKYGGEVVRAGNIIIRQRGTRVHLGQNVGMGKDHTIYSLIDGVVKFEQKDKNRKKVSVYAAS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must possess structural constituent of ribosome to support translation under certain conditions. The designed protein sequence is ",
    "response#1": "GGDEVIVLTGRDKKRRGVGV",
    "response#2": "SFRL",
    "response#3": "SCCAP"
  },
  {
    "reference": "MSFLTTPKMLSEYQCLKNIGFSHKTVLKRRLFRKECTPALKSFYSTQDPALNEVRTEKLKNGVTYVCDPRPGHFSGLGVYVKAGSRYETKKFSGVSHFMDRLAFQATERTPVGEMKAKLENLGGNYMCSTSRESMIYQAAVFNDDVKSMSKLLAETVLAPKIQEDDLVHYRDSIIYENSELWTKPDALLGEFAHVTAFQNNTLGNCLLCTPDKVNGITATSIREYLKYFYRPEHLTLAYAGIPQEIAKEITKELYGHLPSSSLPPLEAIPSHYTGGFMGIKKSEAPPVPYQQEFTHVVIAMEGLPVTDPDIYALACLQFLLGGGGSFSAGGPGKGMYSRLYLNVLNQYPWVETCMAFNHSYTDSGLFGMFVTILDDAAHLAAPLIIRELCNTVLSVTSEETERAKNQLKSSLLMNLESRMISLEDLGRQIQTQNGLYITPKEMIEKIDALTPSDLSRVARRVLTGNVSNPGNGTGKPTVLIHGNVDEVGDVFALCKKAGIGH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Substrate recognition and binding subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins..2. A protein that localizes to mitochondrial processing peptidase complex, mitochondrion and can perform metal ion binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "LRSVHDEGVRREVYLATRTSSSRQLHRLEELLSKRAELAQLSGHASFGHMTLSDKMAKSPEAVSNFLTALVGSNREYVQEELSKLQAMKGGSPLQPWDHAYYVHQRVLQYSQSRRSRELSAVPEFFSLGTVMQGLSRLFDRLYGVRLVPQETAAGETWNPDVRRLDVVDEAERHIAVIYCDLFSRPNKHPNPAHFTLRCAREISSEEVAECATMDHSAHPNDGMATAVDPQSKTLRQLPTIALVCDFAEPPATGAGRPSLLSEHSVRTLFHEMGHALHSILGQTRLQSISGTRCATDFAELPSVLMERFATEPAVLSMYARHWQTDQPLSESMMLSMEKDRLAHGSIYGAVENEAQILMALVDQAYHSIPADKAGQIDSTAIYHQVSSAHSTLPDPTDSRPPTSWQGFFGHLYGYGATYYSYIFDRAIANKIWEDVFQAGKAAVDREAGERYKNEVLRWGGGRNGWDCVAGVLGSAW",
    "response#2": "LRSVHDEGVRREVYLATRTSSSRQLHRLEELLSKRAELAQLSGHASFGHMTLSDKMAKSPEAVSNFLTALVGSNREYVQEELSKLQAMKGGSPLQPWDHAYYVHQRVLQYSQSRRSRELSAVPEFFSLGTVMQGLSRLFDRLYGVRLVPQETAAGETWNPDVRRLDVVDEAERHIAVIYCDLFSRPNKHPNPAHFTLRCAREISSEEVAECATMDHSAHPNDGMATAVDPQSKTLRQLPTIALVCDFAEPPATGAGRPSLLSEHSVRTLFHEMGHALHSILGQTRLQSISGTRCATDFAELPSVLMERFATEPAVLSMYARHWQTDQPLSESMMLSMEKDRLAHGSIYGAVENEAQILMALVDQAYHSIPADKAGQIDSTAIYHQVSSAHSTLPDPTDSRPPTSWQGFFGHLYGYGATYYSYIFDRAIANKIWEDVFQAGKAAVDREAGERYKNEVLRWGGGRNGWDCVAGVLGSAAP",
    "response#3": "LRSVHDEGVRREVYLATRTSSSRQLHRLEELLSKRAELAQLSGHASFGHMTLSDKMAKSPEAVSNFLTALVGSNREYVQEELSKLQAMKGGSPLQPWDHAYYVHQRVLQYSQSRRSRELSAVPEFFSLGTVMQGLSRLFDRLYGVRLVPQETAAGETWNPDVRRLDVVDEAERHIAVIYCDLFSRPNKHPNPAHFTLRCAREISSEEVAECATMDHSAHPNDGMATAVDPQSKTLRQLPTIALVCDFAEPPATGAGRPSLLSEHSVRTLFHEMGHALHSILGQTRLQSISGTRCATDFAELPSVLMERFATEPAVLSMYARHWQTDQPLSESMMLSMEKDRLAHGSIYGAVENEAQILMALVDQAYHSIPADKAGQIDSTAIYHQVSSAHSTLPDPTDSRPPTSWQGFFGHLYGYGATYYSYIFDRAIANKIWEDVFQAGKAAVDREAGERYKNEVLRWGGGRNGWDCVAGVLGSAY"
  },
  {
    "reference": "MTWNLFKKKPEPIVGAEPTSTMTMAPAPAMSTVADNSTEAEGPMGNKKEDYKELMNKFYNQIVKIPLPPWALIAIAVVAGLLILTCCFCICKKCCCKKKKNKKEKGKGMKGALNMKNMKGGENKQDDDDDDAETGLTEEEKEEEEKEQEKLGKLQYSLDYDFQDNKLTVGIIQAADLISMDMGGTSDPYVKVFLLPDKKKKYDTKVHKKTLNPVFNESFVFKVPYQELGGKTLVMAIYDFDRFSKHDIIGEVKVPMNTVDLGQPIEEWRDLESAEKEEPEKLGDICISLRYVPTAGKLTICILEAKNLKKMDVGGLSDPYVKIHLLQNGKRLKKKKTTVKKNTLNPYYNESFSFEIPFEQIQKIQAVITVLDYDKIGKNDAIGKIFVGCSATGTELRHWSDMLANPRRPIAQWHPLKPEEEVDAILAALAGKK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a specific function in cellular response to calcium ion and should be localized to plasma membrane, axon, exocytic vesicle in order to carry out this function efficiently.2. The protein must exhibit the following characteristics: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse; It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone..3. The designed protein must have at least one C2 domain. The designed protein sequence is ",
    "response#1": "MCMVIFAPLFAMFAFATCGGYSGGLRLSVDCVNKTESNLSIDIAFAYPFRLQQVTFEVPTCEGKEQQKLALVGDSSSSAEFFVTVAVFAFLYSLAATVVYIFFQNKYRENNRGPLIDFIVTVVFSFLWLVGSSAWAKGLSDVKVATDPKEVLLLMSACKQPSNKCMAVHSPVMSSLNTSVVFGFLNFILWAGNIWFVFKETA",
    "response#2": "MCMVIFAPLFAMFAFATCGGYSGGLRLSVDCVNKTESNLSIDIAFAYPFRLQQVTFEVPTCEGKEQQKLALVGDSSSSAEFFVTVAVFAFLYSLAATVVYIFFQNKYRENNRGPLIDFIVTVVFSFLWLVGSSAWAKGLSDVKVATDPKEVLLLMSACKQPSNKCMAVHSPVMSSLNTSVVFGFLNFILWAGNIWFVFKETR",
    "response#3": "EKDIFVVRENGTTCLMAEFAAKFIVPYDVWASNYVDLITEQAEISLTRGAEVKGRCGHNESELQVFWVDRAYTLKMLFVKESHNTSKGLEATWKLSKVQFVYDSSEKTHFKDAVSAGKHTANSHHLSALVTPAGMSYECQAQQTISLASSDPQKTVTMILSAVHIQPFDR"
  },
  {
    "reference": "MPTTKRLNVAVVGATGMVGQEMLRILAERHFPSERISALASERSKGLSVPYNGSSITVEPLNEHAFRGVDVALFAASGDIGLMYGPLAVEAGSLVIDNSSAWRMKENVPLVVPEVNSEDIRDNEGIIANPNCCAIPLTVVLNPLGQAAGIERVLVSTYQAASGAGRALVDELEEQTKAIAGGAKPQVSAYPYQLAYNVVPGGWRAEPEGYNEEEIKIVNETRKILHMPELRITATCVRVPVPIGHGESVYLETKEKLSADQARTLFASAPGVIVEDDPHAKLYPTPHHVAGKDEVYVGRIRNDPSTARGLALWLVSDNLRKGAALNAVQIAEHAIKMGILA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a high affinity for NADP(+), phosphate.2. A protein that can modulate the overall flux of metabolites through the Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5, L-threonine biosynthesis pathway, enhancing or suppressing its activity as needed.3. The designed protein must possess NADP binding, NAD binding to support diaminopimelate biosynthetic process, threonine biosynthetic process, isoleucine biosynthetic process, 'de novo' L-methionine biosynthetic process under certain conditions.4. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..5. The protein contains novel Semialdehyde dehydrogenase NAD-binding domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "RVAVVGATGNVGREMLNILAERKFPASEVIALASARSQGQEISFGDKRLKVQNLEHFDFKGVDFALMSAGSAIAKEYGERIGATGCIVVDNSSFWRYHGDVPLVVPEVNAHVLDADDFTVPDNVHIIANPNCTTIQMVVALKPLHDLATIKRVVVATYQSVSGAGKEGVDELWDQTKGVFVNDKPEPRKFTKQIAFNVIPHIDVFMEDGYTKEEWKMVAETKKILDPKIKLTATCVRVPVFVGHSEAVNLEFANPISADEAREALREAPGISVIDKREAGGYATPVEAAGEYDTYVSRIREDVTVENGLSLWVVSDNLRKGAALNAVQIAELVLSL",
    "response#2": "MNYVGIVGATGAVGQELIELLYKRQFPLKELRLFASSRSAGKTITWEGKSWTVENSDGQDFKGIDIVFFSAGASISKALAPVAVKGGAVVVDNSSAFRMDPDVPLVIPEINPHAIRQRPKGIIANPNCTTLSMIVAIGLKPLHDLATIKRVVVATYQSVSGAGKEGVDELWDQTKGVFVNDKPEPRKFTKQIAFNVIPHIDVFMEDGYTKEEWKMVAETKKILDPKIKLTATCVRVPVFVGHSEAVNLEFANPISADEAREALREAPGISVIDKREAGGYATPVEAAGEYDTYVSRIREDVTVENGLSLWVVSDNLRKGAALNTYEIAEALVEA",
    "response#3": "MARIAVVGATGNVGRLVLKVLSERGFSVDSVVALSSAESAGKRLGYGENSEIRCGSLDEYDFHGSELAIFATSDAVSERYVGRAVQCDCVVIDSSSCYRMDDDVPLVIPEVNGDAIASALAKGGRNWIGGNCTVSCMLMALNPLRAVAGIKRVVASTYQSVSGAGRGAMSELYDQTKAMFVNKKLEPSEFPRQISFNCIPHVGEFLEDGSTTEEWKMSAEAQKIMGEDIKIVATCVRVPVFVCHSQALNIEFHSAISVDAAYEALSEAPGVLVLGNESSMQYVTPIDCVDDDVVYVSRIRKDTTVPYGLSMWVISDNLRWGAAEPLRRMLRIVLGHW"
  },
  {
    "reference": "MGPSQLVRAPRPPGLTSPYRRPGMGGPRRRCPSMFKCSRRTYRQKPRGPTATNPASEATNISDTDTTTSVLILSPRVLRFLCQPGGFLVL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be optimized for 1-phosphatidylinositol-3-kinase regulator activity.2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "DMIRQMMIKIFRCIEPELNNLIALGDKIDSFNSLYMLVKMSHHVWTAQNVDPASFLSTTLGNVLVTVKRNFDKCISNQIRQMEEVKISKKSKVGILPFVAEFEEFAGLAESIFKNAERRGDLDKAYTKLIRGVFVNVEKVANESQKTPRDVVMMENFHHIFATLSRLKISCLEAEKKEAKQKYTDHLQSYF",
    "response#2": "MHKASSSKKSFDDTIELKKNEQLLKLINSSEFTLHNCVELLCKHSENIGIHYYLCQKLATFPHSELQFYIPQLVQVLVTMETESMALEDLLLRLRAENPHFALLTFWQLQALLTDLSTDPASYGFQVARRVLN",
    "response#3": "MHKASSSKKSFDDTIELKKNEQLLKLINSSEFTLHNCVELLCKHSENIGIHYYLCQKLATFPHSELQFYIPQLVQVLVTMETESMALEDLLLRLRAENPHFALLTFWQLQALLTDLSTDPASYGFQVARRVLV"
  },
  {
    "reference": "MHDTNDYLPPRLYDGAQVGALDARFAAEFGVSGRSLMERAARAAYDELVARWPMPGRLVAFCGPGNNGGDGALIAVLAQANGWDVRLVAPAGLDEAEGEAARAFEAYRGAGGRVVAFEDAAIEGADLIIDALLGTGVNRDVEGAMAQAIARINEAGTDGAGILSVDIPSGLDAGTGQRRGDAVQADTTVTFIGVKLGLLTGDGPVHCGALAFDDLGAPDALYVDQPYRARRIAHRDLRRALPARAKSAHKGNNGHGLCLGGDRGMGGAIRLCAEAGLRAGAGLVSVVCHPDHAGAMAQARPELMCLGLSDEADMADRLDAQIAAASVIAVGPGLGQNVWGDRLFEQALASSKPLVVDADALNRLAVDPVQRGHWILTPHPREAARLLGVQTADILADRVAAVRDLADRYKAVVVLKGAGSLVATPDEIWLCAEGNPGMAVGGMGDVLTGVITALVAQNLTLSDAAVFGTYTHALAGDAIAARAGERGMLPSDLVTALPSQLNPVRR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must be able to NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity.2. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. The designed protein sequence is ",
    "response#1": "EGADLAPHLQQRPPDSHKGTFGHLLVVAGSEGMTGAAALAGEAAMRVGSGLVSVAVPQSLRTVIEMKLTECLKLGLEESEPGVLGEGAVRQLTPHLHRYHATLLGPGLSTRGEVARVVVALIERAEHPMVLDADALNILAGTLESLEVAKAPLILTPHPGEMARLLRTSTARVQENRLSIAHDFATRHGVVLVLKGARTLIATPEGEVFVNPTGNAGMASGGTGDVLAGMIGGFLVQGHPPRTAALLGVYLHGLAGDLAAEALGERSFIASDLLDRLPTAIAQLAGSPDMPGAAILASWAALTSGAGIVRLIHQEGMNAELSAAPLELVRQTYSPQDLEPVIEVMNKSSASFIGPGMGRTPDAFLVLKTLFKYLSKPCVIDADALTMLSEKEVPLPPSAVMTPHHGEMLRLLGKDKXYFSTEQLLQLAKNFAXRKKITLVLKGAPTFILQKGHPIYVNPRGSPGMATAGSGDVLTGMIAAFXAQHLQPIQAAIVAVYLHAVAGEYAALXKTEHCLIASDIL",
    "response#2": "AGPMPGAAALAASAVLRAGGGYAILSGATSVPFSAIIAERGENFAERLLDERLGAVVIGPGYPPGAALDRDVAAALKSGKPLVLDAAAIPSALPRLGDTPAILTPHAGEFARAFPGLEGSKIEQARAAARRSGAVLVYKGADTIIAAPDGRIVAAWPGCPWLATAGTGDVLAGACGAMLARGGDAFDAAVAAVSWHISRAL",
    "response#3": "HKYERGYVTGLAGSPDMPGAAILASWAALTSGAGIVRLIHQEGMNAELSAAPLELVRQTYSPQDLEPVIEVMNKSSASFIGPGMGRTPDAFLVLKTLFKYLSKPCVIDADALTMLSEKEVPLPPSAVMTPHHGEMLRLLGKDKXYFSTEQLLQLAKNFAXRKKITLVLKGAPTFILQKGHPIYVNPRGSPGMATAGSGDVLTGMIAAFXAQHLQPIQAAIVAVYLHAVAGEYAALXKTEHCLIASDID"
  },
  {
    "reference": "MAETIDHRMAAGVMQFVTQKFPRLARIAMLVGAGGNGRQVLALAERYKKTGVGVTLVRPGDAVDVEELRTHDVLIDGLVGTGLHGPLSAVYHDLVGRAQASRRPIIALDGPTGLDLGTGCPQPDTLRADYTLFCGGAKIGALTGAGRAWCGETTTIDVGQPYPAGYLRALIPDNADLRALLPLRRVDMHKGDAGTLVIAGGGLGMPGAVRLAAVAAYRVGAGLVVAGVHERNAEVLAAAFPECIAFAASSAELPWGRAQALLVGSGLGRGDWAQDVWQRARTLGRPMVVDGDGLYWLAHAPESRPDWVLTPHGGEAARLLGVTSADVAADRPGAAVELQRRYGGVCVLKGAGTLVAGGDILWLCPFGNPGMATAGTGDVLAGTIAGLMAQGLSNLAAARLGTFVHARAGDRAARRGPPRGLLASDVAAQLRAALGALC",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The AMP binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must exhibit the following characteristics: Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. The designed protein sequence is ",
    "response#1": "DSPSIIKQIASRDPCAHKGLQGKALLVGGDYGLGGAILIATRACLKSGAGLTACLTEQRNISVGLVTSPEAMFANWKLHALEQRLTWCDAIALGPGLGQGEHALQLFSRTSRSNKVKVLDADALKLLARESNIDNSRIITPHPGEAAELLACTIAEIESDRYRASRALQTQYGGVVVLKGAGTIVTDSDCHYVCRAGNAGMATGGMGDALSGILVALLARGIEITMAARLGVMLHSHAADLNVSAHGMCGLTASDVVDTLREAIFQP",
    "response#2": "ERTDMVRLIKNRKPFSHKGTYGHALLIAGNIDTMGAALLSAKACLYAGAGLTTACIPATGLTALNTLLPEVMALPRDEYTRIENPAKYKAIAIGPGLGINSDNEKLLESLILAARPLVIDADALNILGERPDLLDILPAGSILTPHVKEFDRIFGAHESWWARVQTAIIWARKKQVVIILKNQFSFVCVPNGKVLINPTGNPAMAQGGMGDVLTGILSALLAQGYSATDSAKIGCYIHGLAGDYLASERHVVTASEVAERIPFEMHLLSQ",
    "response#3": "VLLVAGSLGKSGAAILSGRGALRTGAGLVTIATPDIVLPHVAGGQPEYMSESLESTDEGTVAFSSLEGNSFEEILKGKTVLGIGPGLGTHTETQKFIRTVVRDTPLPTILDADGLNAFAGSGDLFRDRQSSFLALTPHPGEMGRLLGISTQTIQADRLAMALDAARRWNAHFVLKGHHSIVASPDGQVFVSTAGNAGLAKGGTGDVLTGILAALLAQFGANDGARVLSLGVYLHGLAAELASHGTDLSGLLAGEVADAVC"
  },
  {
    "reference": "MAPQRSQDKTPIQLLHEYGIKISSAPKYELIHADGDAHQPSFMFSVTIGEVTCKGRGSTKKAAKHEAAEAALKLLKRDSQIIDQRDNNGLSPEAGEASNPVGILQELAMQRVWCLPEYVVFMETGPGHMKEFTIACRLEGLEETGSGSSKKLARRAAAENMIAKLQSLSGSSEITWSPPSRVYVESLRNSTGEKVSLLKRTPLSLPNTDYIQMLLEISLELGFQVTYIDIDELTVNGQYQCLVELSTRPVTVCHGSGVTSSNAHNAAAHNALQYIKMVASKH",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must have at least one DRBM domain.2. The protein must exhibit the following characteristics: Activates eif2ak2/pkr in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of eif2s1/efi2-alpha and inhibition of translation and induction of apoptosis; Does not seem to be required for processing of pre-miRNA to miRNA by dicer1 .; Required for siRNA production by dicer1 and for subsequent siRNA-mediated post- transcriptional gene silencing.3. A protein that can perform double-stranded RNA binding, siRNA binding in cytoplasm, nucleus, perinuclear region of cytoplasm, RISC complex, RISC-loading complex to support regulation of production of small RNA involved in gene silencing by RNA, siRNA processing. The designed protein sequence is ",
    "response#1": "VVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLSAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLSAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLPFVREFGIMVKDDMTDVTGRVLQPPSILYGGRVWEEPNAPLNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKTFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYTGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLSAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYH",
    "response#2": "LVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLSAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLPFVREFGIMVKDDMTDVTGRVLQPPSILYGGRVWEEPNAPLNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKTFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYTGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLSAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTM",
    "response#3": "VVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLSAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHPGCCSVLSELSEEQAFHVSYLDIDELSLSGLCQCLVELSTQPATVCHGSAPTREAARGEAARHALQYLKIMLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLSAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLSAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHL"
  },
  {
    "reference": "MMNDMRNDDPNFNVMGNFDHGLTLALSKGRILKETLPLLEAAGINLLEDPEKSRKLIFPTTHQKVRILILRASDVPTYVENGAADFGVAGKDVLMEHGAQHVYELLDLKIANCKLMTAGKVGMQHPKGRLKIATKYVNLTRQYYASLGEQVDVIKLYGSMELAPLVGLGDYIVDVVDTGNTLRANGLEPLEEIMKVSSRLIVNKASFKRKQALLDPILAQVEEAVNQR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein can be expressed in cytoplasm and perform ATP binding without triggering undesirable cellular responses.2. The protein should have a low Km value for 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-   alpha-D-ribose 1-diphosphate + ATP.3. We require a protein that can specifically bind to the metabolites produced in the Amino-acid biosynthesis, L-histidine biosynthesis, L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9 pathway.4. The protein must exhibit the following characteristics: Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.; Catalyzes the condensation of ATP and 5-phosphoribose 1- diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). The designed protein sequence is ",
    "response#1": "LLALPNKGRISKPVNEILEKSGLKISVHGRSLFAQTVDPEIKVMFARAKDIPEFVRDGVADVGVTGYDLMLERDTEEELEMLLDFKFGNARLVIAAPENSSVNSIDDVKDGMKIATEFPGLTKRYLEKKGLNLEIIELSGATEIAPFIGVSDLICDLTSTGTTLQLNRLKEVENVVSSTTRLVRATDVPVYVEYGQAQLGIAGYDVLLEKSPDVANLIDLKFGYCRMSVAVPADSPYQSPLDIPHHGKVASKFVNCAKDYFRRLDIPVEIIPLYGSVELGPITGMSEAIVDLVSTGRTLRENGLVEIDELFASSARLIAHPLSYRLDRDQIYNWVEKLEPRPERDDDIPGLVFDGVVDLGIIGENVLEEEELTRQAAGETVNYKKLRRLDFGGCRLSIAIPQDEAYNGISDLKNARIATSYPNLLKRYMQQQGVDFKTCSLTGSVEVAPRAGLADAICDLVSSGATLEANGLKEVEIIYRSKSCLIQRAAELSPEKQALVDKLLTRIQKAPDVPTYVEHGAVDMGIVGKDVLLEANKQLYEVLDLQFGKCKFSVAGSTSMENYPTPHMRVATKYPNIAKNFFQSKGQSVEIIKQEGSVELAPMVGLADVIVDIVETGNTLRENGLMVFEDIVEISARLVLNQVSYKTKHQEIRGLIDRIKAPDVTTYVRHGVADIGVVGKDVLIEHPTGYLEMLDLNFGLCKFSVASTPDYNPSDHKRKRIATKYPTVARDFFNQKGEDVEIISIQGSVEIAPVIGLADAIVDIVETGNTLVANGLEVFEDICRISARMIVNKASLKNKPELLAFIKK",
    "response#2": "MQLLALPNKGRISKPVNEILEKSGLKISVHGRSLFAQTVDPEIKVMFARAKDIPEFVRDGVADVGVTGYDLMLERDTEEELEMLLDFKFGNARLVIAAPENSSVNSIDDVKDGMKIATEFPGLTKRYLEKKGLNLEIIELSGATEIAPFIGVSDLICDLTSTGTTLQLNRLKEVENVVSSTTRLVKPMDVVTYVEHGVADLGIAGKDVMMEEERDVYELLDLKISKCHLAVAGLPGVRMNQIAPRVATKYPNIASSYFREQGEQVEIIRLNGSIELAPLIGLADRIVDIVSTGRTLKENGLVELEKIADVTSRLIVNPVSYRMKDEAVDELVHRLRDDDIPGLVFDGVVDLGIIGENVLEEEELTRQAAGETVNYKKLRRLDFGGCRLSIAIPQDEAYNGISDLKNARIATSYPNLLKRYMQQQGVDFKTCSLTGSVEVAPRAGLADAICDLVSSGATLEANGLKEVEIIYRSKSCLIQRAAELSPEKQALVDKLLTRIQEASA",
    "response#3": "LLALPNKGRISKPVNEILEKSGLKISVHGRSLFAQTVDPEIKVMFARAKDIPEFVRDGVADVGVTGYDLMLERDTEEELEMLLDFKFGNARLVIAAPENSSVNSIDDVKDGMKIATEFPGLTKRYLEKKGLNLEIIELSGATEIAPFIGVSDLICDLTSTGTTLQLNRLKEVENVVSSTTRLVKPMDVVTYVEHGVADLGIAGKDVMMEEERDVYELLDLKISKCHLAVAGLPGVRMNQIAPRVATKYPNIASSYFREQGEQVEIIRLNGSIELAPLIGLADRIVDIVSTGRTLKENGLVELEKIADVTSRLIVNPVSYRMKDEAVDELVHRLASEIAREIGSGAVDFGVTGEDLVREGLADADARVEFAARLGFGHADVVVAVPEIWYDVDTMADLGDVAADFRARHGRRLAIATKYWRLTQQFFSGSHGIQLYRIVESLGATEGAPASGSADIIVDITSTGSTLKANHLKILSDGVILRSEACLVRARKAEHGGNPLIDRILAARATDVPVYVEYGQAQLGIAGYDVLLEKSPDVANLIDLKFGYCRMSVAVPADSPYQSPLDIPHHGKVASKFVNCAKDYFRRLDIPVEIIPLYGSVELGPITGMSEAIVDLVSTGRTLRENGLVEIDELFASSARLIAHPLSYRLDRDQIYNWVEKLFA"
  },
  {
    "reference": "MSQFDNVSVVKKANVYFDGKCVSHTVILADGTRKTVGVILPSTLTFNTGAPEVMEGVGGSCRVRLKGENDWKTWAEGQSFQVPGDSSFEIACDEPYHYVCHFG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit uridine phosphorylase activity. The designed protein sequence is ",
    "response#1": "KFVCVCGSSGRIHNFAISMAKLAGLALPVENIAGSHARFVLYKVDHILFADHGIGIPSTLILMHEVTKLLYYAGCKDVLFIRLGTSDGLGVKPGTIVLSDRCVNTKLEPYNELCILGKPVRRQTKVDSNAVNELKKLSENLSLKCSVVVGGTITANDFYEELGRLNGSICTFSKEEKLAFLQSVYDHGIRNMEMEGAAITSHCNLTGHRAILVCVTVVNRLETDEVTTSTDEFKLFEC",
    "response#2": "KFVCVCGSSGRIHNFAISMAKLAGLALPVENIAGSHARFVLYKVDHILFADHGIGIPSTLILMHEVTKLLYYAGCKDVLFIRLGTSDGLGVKPGTIVLSDRCVNTKLEPYNELCILGKPVRRQTKVDSNAVNELKKLSENLSLKCSVVVGGTITANDFYEELGRLNGSICTFSKEEKLAFLQSVYDHGIRNMEMEGAAITSHCNLTGHRAILVCVTVVNRLETDEVTTSTDEFKLFEC",
    "response#3": "KFVCVCGSSGRIHNFAISMAKLAGLALPVENIAGSHARFVLYKVDHILFADHGIGIPSTLILMHEVTKLLYYAGCKDVLFIRLGTSDGLGVKPGTIVLSDRCVNTKLEPYNELCILGKPVRRQTKVDSNAVNELKKLSENLSLKCSVVVGGTITANDFYEELGRLNGSICTFSKEEKLAFLQSVYDHGIRNMEMEGAAITSHCNLTGHRAILVCVTVVNRLETDEVTTSTDEFKLFEG"
  },
  {
    "reference": "MAATADREKALATALQQIEKQHGKGSIMRLGEQETVKIAAIPTGSVALDVALGVGGLPRGRIVEIYGPESSGKTTVALHAIANAQAEGGICAFIDAEHALDPEYARKLGVDTDSLLVSQPDNGEQALEIADTLVRSGALELIVVDSVAALTPKAEIEGEMGDSHVGLQARLMSQALRKMTGALNAAGTTAIFINQLREKIGVMFGSPETTTGGRALKFYSSVRLDVRRVETLKDGSEMVGNRTRVKVAKNKVAPPFKQAEFDILYGQGISREGSLIDMGVDCGIITKSGSWFSYNNEQLGQGKENVRKFLRGNPDVANEIEDKILTHLGLREAEVPEGVDPRTGEVEF",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The ATP binding site should be located in a region of the protein that is accessible to the ligand.2. The protein should be able to modulate DNA recombination in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "QGKSSLLHVMERTSTPMGGRLLRNTLVNPFYDQKEILLRQDAVEFLLDRSELRKNLKSLLSQVRDLERLTTKITTSLAGPKDIGMLRDSLNASIRVCEVLSPLPLPKFFQGRFTLPIGLTSLSELLSRALLGELPLRISEGNIFCDNHHPDLQRLRYTKEHSKEWLWQYQETIRQQTGVKKLKVCYSQALGYYIEVSSDFAPLLPKEFIRRQSRLHAERFTTEKLQEFQDDMLNISDKLQTLETQLFKDLCAQILQQREEILSLSQVIADIDYILSLSDLAAEYNYCRPIVDTSDSLSISGGIHPVAQTA",
    "response#2": "IVTGPNMSGKSTYIRQVGLIAVMAQIGSFVPADDAEIPIFDRVFTRMGARDDISTGKSTFLIEMSEVALILEKATKKSLVLLDEVGRGTSTFDGISIAWAMSEYIYNEIGCETMFATHFTELTELSDVYEGIKNLTIEVRETNNGVVFLHKVVEGIADRSYGIEVAQIAGVPDGVD",
    "response#3": "QGKSSLLHVMERTSTPMGGRLLRNTLVNPFYDQKEILLRQDAVEFLLDRSELRKNLKSLLSQVRDLERLTTKITTSLAGPKDIGMLRDSLNASIRVCEVLSPLPLPKFFQGRFTLPIGLTSLSELLSRALLGELPLRISEGNIFCDNHHPDLQRLRYTKEHSKEWLWQYQETIRQQTGVKKLKVCYSQALGYYIEVSSDFAPLLPKEFIRRQSRLHAERFTTEKLQEFQDDMLNISDKLQTLETQLFKDLCAQILQQREEILSLSQVIADIDYILSLSDLAAEYNYCRPIVDTSDSLSISGGIHPVAQTL"
  },
  {
    "reference": "MKELVLASHNAGKLRELQALLGNSITIRSIGEFSTVEPEETGLTFIENAILKARNACKVSGLPALADDSGLTVDYLHGAPGIYSSRYSITGGDAGNNRKLIEALQGVPDEQRGAQFICVLALLQHADDPLPIICEGRWQGRILHTPQGEHGFGYDPLFYVEQHQCSSAELPSALKNQLSHRAQAMQLLKQQLSL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be able to modulate nucleotide metabolic process in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "DHKYARGHLTILAGPHATGAARLAALAGRRVGAGLVTIAAFRSTLPAFQAAEPGNLIVEADDGTMFAHLLGDPRRNAVLIGPGSGTDGRTRESVLSALEARRSVVLDADAITVFAADPDLLFRSVRGPTLLTPHEGEFRRLFPDLDGVVGKLERVRRAAARSGATILLKGPDTVIAEPGGRAIINVHASPALATAGSGDVLAGTIGGLLAQNLTPFAAASAAAWLHGECALTFGGSGLIAEDLPALLS",
    "response#2": "DAHKGTFGRVFIIAGSVGMTGAVALSGLASVRGGAGLVTVGIPESLNDILEVKLTEAMTLPLAESTNRCISNAALNAALEFASKCNVVVLGPGISTNEETKQFVLEFVQNCEVPMVVDADGLNILAEYPHILANAKAKVVITPHAGEMAKLLASTPSKIQEDRLKAAKTAAQRYNCVVVLKGARTLIANPEGMLCINPTGNAGMASGGSGDVLSGIIGAFLARGMQPYNAAAAGVYIHGLAGDIAAQNKGKISLAAGDIIEYH",
    "response#3": "FRLMDFGTRRRFSRQVQFEVIAELQREFPYLIGTSNYQLARERGLPPIGTQAHEWFQAHQQISPDLANSQSAALQAWLDEYPDRLGIALTDCITMDAFLRDFGPSFARAYQGMRHDSGDPVEWGEKAIAHYQQLGIDPLTKTLVFSDSLDFDKAFMLYRHFWQRINLVFGIGTRLTCDIPGIKPLNIVIKLVKCNGKPVAKLSDSPGKIMCHDKAFVRALRKAFDVG"
  },
  {
    "reference": "MKYMTVTDLNNAGATVIGTIKGGEWFLGTPHKDILSKPGFYFLVSKLDGRPFSNPCVSARFYVGNQRSKQGFSAVLSHIRQRRSQLARTIANNNVPYTVFYLPASKMKPLTTGFGKGQLALAFTRNHHSEYQTLEEMNRMLADNFKFVLQAY",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein with DNA binding is needed to enhance inhibition of host DNA replication by virus in a specific context.2. The protein must exhibit the following characteristics: Only the architecture of the nucleoid is affected; Disorganizes the host nucleoid and inhibits replication, but without host DNA cleavage or degradation; May act on the host chromosomal sequences that determine the structure of the nucleoid. The designed protein sequence is ",
    "response#1": "TKKEVSIKTTLKELVHKRVTSPEDWMMMQPDSYIEMMAQPGGENLLKNTLEICTLTLARTKTAFDLILEKAETSKLTNFSLPDTRTCKIFAFHGWNYVKVCHAICCVLNRQGGKRNTVLFHGPASTGKSIIAQAIAQAVGNVGCYNAANVNFPFNDCTNKNLIWVEEAGNFGQQVNQFKAICSGQTIRIDQKGKGSKQIEPTPVIMTTNENITVVRIGCEERPEHTQPIRDRMLNIHLTHTLPGDFGLVDKNEWPMICAWLVKNGYQSTP",
    "response#2": "TKKEVSIKCTLRDLVSKRVTSPEDWMMLQPDSYIEMMAQPGGENLLKNTLEICTLTLARTKTAFELILEKADNTKLTNFDLANSRTCQIFRMHGWNWIKVCHAIACVLNRQGGKRNTVLFHGPASTGKSIIAQAIAQAVGNVGCYNAANVNFPFNDCTNKNLIWIEEAGNFGQQVNQFKAICSGQTIRIDQKGKGSKQIEPTPVIMTTNENITIVRIGCEERPEHTQPIRDRMLNIKLVCKLPGDFGLVDKEEWPLICAWLVKHGYQSTMS",
    "response#3": "TKKEVSIKCTLRDLVSKRVTSPEDWMMLQPDSYIEMMAQPGGENLLKNTLEICTLTLARTKTAFELILEKADNTKLTNFDLANSRTCQIFRMHGWNWIKVCHAIACVLNRQGGKRNTVLFHGPASTGKSIIAQAIAQAVGNVGCYNAANVNFPFNDCTNKNLIWIEEAGNFGQQVNQFKAICSGQTIRIDQKGKGSKQIEPTPVIMTTNENITIVRIGCEERPEHTQPIRDRMLNIKLVCKLPGDFGLVDKEEWPLICAWLVKHGYQSTMC"
  },
  {
    "reference": "MIGEDADYASASPELAVAEPALDSRSVRAGDVFFALAGVKSDGLSFVREAIDRGAVAVVAEGARPTWLDPAISYVRVGNARHSMGLAAARAFPRQPETIAAVTGTSGKTSVAAFTRQIWQGLGFSAASLGTLGVVAPSGVAKGALTTPDPITLHRTLHHLAEEGVTHLCLEASSHGLDQHRLDGVRLKAGGFTNLSRDHLDYHPDMQAYLDAKMRLFRDLVPRGGGAAVWVDSTEGSHVAQVAAEHELNVLGIGTTGAGIALLNRVDEGLGQRLEIEIDGARHTVKLPLVGAFQACNALIAAGLALLTGATAREVLPLLETLEGVPGRLELVGTKEGAGVFVDYAHKPDALANALDALRPYASGRLIVVFGCGGDRDRGKRPIMGAIAAAKADVVIVTDDNPRSEDPATIRAAILAHAPGAREIGDRAEAIKAALDEAAPGDVVLIAGKGHETGQIIGDRTLPFSDRDVAVGLLGR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Mg(2+) is required for the proper folding and stability of the protein.2. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..3. The protein design must be able to perform magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, ATP binding in order to contribute to cell cycle, cell division, peptidoglycan biosynthetic process, regulation of cell shape, cell wall organization.4. The protein design should take into account the physiological conditions of the peptidoglycan biosynthesis, Cell wall biogenesis pathway.5. Incorporating a Meso-diaminopimelate recognition motif motif is critical for the successful design of the protein.6. The protein should be able to bind UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP, meso-2,6-diaminoheptanedioate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MRLIELLSIRNLVADSRQIKPGDTFVAYPGDTTDGRKFIPQAIAAGANAILWDPRDDNPPLHLLVDNPSRSLAVAAARFYPDQPTCVVGITGTNGKTTVTALIESIARAAGLKCAVLGTLGNGFPDDLEKTTHTTPDALMLHRILRKMVEQGIQYCAMEVSSHGLAQRRVQGCDFDVAVFTNLTRDHLDFHETMEDYFAAKKRLFAGLKPTAVINIDDEYGQRIWESCQATVISYGLTHPADVRASDVALSANGIRFTLHTPWGTADVSLPLIGRFNVYNALAAFAACLTQGIPLDVARRALSQVKAVPGRLELVAHTEDGALAYVDYAHKPDALENVLNSVRPFTTGRVIVVFGCGGDRDRGKRPIMGEIACRLADVVIVTDDNPRSEVASEIRAEIMAAAPTAIEMGDRAAAIRHAVSMLSSGDTLIVAGKGHEDYQIIGHTKHHFDDRLVAKEALSE",
    "response#2": "MSLKELFSKAEGIKEDSRQIKPGDLFVAIPGYHTDGRFFIDKAIAKGASAVLAETEKKNLDGSIYYRDNVPVIYLQKLSHIICEIASRFYKRPSDKFHLFGVTGTNGKTTVTTLLHELYTELGFRAGLLSTVEVRIGREKHPATHTTPDALAIHGHLAEMRDAGVDYVFMEVSSHAVHQRRTLGLDFNGGVFTNLSHDHLDYHGTFAEYINAKKAFFDQLPATAFALVNADDRRGGVMVQNTAARVHRYSLRSNVDFHARLLANTPQGLQLDLGGTEVFFKLLGRFNAYNLLAAYATAIVEGVSLSNIAKSLSLAPKIPGRLEHVHDPAGRITALVDYAHTPDALRNVLETLRALLPDGHRLSCVVGAGGDRDRSKRPEMARIAAELADQVILTTDNPRSESPEAILDEMEAGVPPAAAPRVLRITDRRQAIRTAVRLGHEVLLVAGKGHETYQIINSKKVPFDDRLVAQEALL",
    "response#3": "MKLKELSKIIGGEIQGTEEIEINGLSLDSRSTSKGDLFIALQGLQQHGLEYSQQAIARGAVALAWESSRQVEWSVTFIGLDSQNIEPGGLFAALPGTRVHGAKFAFDSAAGAILTDDAGLAIIEQTSDRRPVLVVDDVRAILGHVAAEFFHPFPKEKMTMIGVTGTNGKTTVVTLLYLLYSQLGKKAGLLSTVRNKIISETIPSTHTTPDAIAINQLLAQMVEKGCTHCFMEVSSHSIQQNRISGLDFDLALFTNLTHDHLDYHKTFEAYINAKKQFFDGLSSGSIALTNADERHGNTMVLNTKAVVKKYGLKAMADYKCRLVENSFEGLQLNIDGQDVWTRLVGSFNAYNLLAVYSTAILLGQDKIEVLRSLSMLHPVAGRMEKYYESNQSIIIVDYAHTPDALEKALCTLRELLRIEGRDARLFCVVGCGGDRDKGKRALVGEVATRLADEVIFTSDNPRSEDPLRIIKDIAAGAVKGNYQLEVDRALAIYQAIANARNGDIVLIAGKGHETYQEIKGVRYPFDDRQVAREFLKELM"
  },
  {
    "reference": "MPDFSIENEILKLKNNKECIIVGVDEVGYGSLAGPVVSAAVFFPNYNNETTQDINDSKKLTPKTRQKIYNKIITRVKWSIGFAHIFEIDEYNILNATHIAMKRALTGLNAHIDIDYVIIDGNKIPNIPWNAQAIIGGDTISTSIAAASIIAKVTRDRLMETLHIQYPQYNWNKNKGYGTKDHITSLYKYGKTIHHRNTFTPISKISYMFKNS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must bind to Mg(2+), Mn(2+) in order to perform its enzymatic function.2. The protein must exhibit the following characteristics: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids..3. The designed protein should have a high specificity for a divalent metal cation binding, with minimal off-target effects.4. The protein contains novel RNase H type-2 domains that confer a unique function or activity.5. The protein should have a fast turnover rate for Endonucleolytic cleavage to 5'-phosphomonoester..6. A protein that can perform RNA binding, RNA-DNA hybrid ribonuclease activity, manganese ion binding in cytoplasm to support RNA catabolic process. The designed protein sequence is ",
    "response#1": "MSVIGSDEVGTGDYFGPITVACAYADKTKLSLMKELGVKDSKDLKDPQIIEIAKLLIKTIPYSLLVLKNEKYNQMQEKGMSQGKMKALLHNQAISNLLRKMNGVKPEAILIDQFAEPGIYFKHLSGRDIVKEKTFFSTKAESIHLSVAAASIIARYSFLIEMDKLSREAGMTIPKGAGPHVDEAAAKLIINKGEGALRTFTKLHFANTQKAKRLVEG",
    "response#2": "GPVAGVDEAGRGSCCGPITIAACIMPQRPIAQLQALTDSKKLSAAKRAELYPLIKKYALAWSIIHISAAEIDREGIQHANIFGMRRAIEKLDVAPGYVLTDAMKVPGLRCPSLPIIGGDAAVRCIAAASVLAKHSRDLIMDDLGGKYPEYGLEDHKGYGTKSHMDAVRHHGATPEHRYSYSNVKAAHDQWLK",
    "response#3": "IGLDEAGRGPVLGPMVIALVKIKGQDLKNFEKLDIKDSKKHSKNRREELFEMIINNYDVRYEILEAKTIDKLMNTINLNKIELMAFTKLINSVLKEEYPNYKENSKIQTATPQIEIYIDACSSNEKAFANQIKSKLIVNDENNIKIIAEHKADENYKIVSAASIIAKVIRDRKIEEYKKIYGEIGSGYPSDKITQKYLANYVEKNRELPEIARKSWNF"
  },
  {
    "reference": "MSGHSKWSTIKHKKGAADAKRGKIFTKLIKEITVAARMGGGDIDSNPRLRGAVAAAKAQNMPKDNLERAIKKGTGDLEGVDYEEILYEGYGPGGVAILVECLTDNKNRTIADVRYIFSKAGGNIGTDGCVAWMFDKKGLITISKEESDEDTLMEVGLEAGAEDVTDEGDCFEIITDPADFDAVKGAVEAAGIKIEMAEVTMIPQTQTRLEGKEAEQMVRFMDALDDCDDIQKFYSNADIPDEVYDAM",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have DNA binding within cytoplasm to regulate regulation of DNA-templated transcription. The designed protein sequence is ",
    "response#1": "PDSQRLATGGLDGKIRIWSIPDILKFASNPNASTDKDILMKPLSTMSRHAGSVTTVKFSPDGKYLASGSDDRILLIWELEGGTTQPMFGAESTDIEHWNVRRRLVAHDNDIQDICWAPDSSIMVSVGLDRAIIIWNGSTFEKVKRFDVHQSHVKGVVFDPANKYFATASDDRTIKMFRYHKTGETSFSVEHVITEPFKGSPLTTYFRRLSWSPDGQHIAAPNAMNGPVSTVAIIERGTWESPVSLVGHDQPTEVASFNPRIFKRQKDDSTTDTIDGKKTGISDEVDCIVASSGQDKTLAVWSTSKARPLIVAQDICGKSITDMSWTPDGKILFITSLDSSIVVLTFEDNC",
    "response#2": "PDSQRLATGGLDGKIRIWSIPDILKFASNPNASTDKDILMKPLSTMSRHAGSVTTVKFSPDGKYLASGSDDRILLIWELEGGTTQPMFGAESTDIEHWNVRRRLVAHDNDIQDICWAPDSSIMVSVGLDRAIIIWNGSTFEKVKRFDVHQSHVKGVVFDPANKYFATASDDRTIKMFRYHKTGETSFSVEHVITEPFKGSPLTTYFRRLSWSPDGQHIAAPNAMNGPVSTVAIIERGTWESPVSLVGHDQPTEVASFNPRIFKRQKDDSTTDTIDGKKTGISDEVDCIVASSGQDKTLAVWSTSKARPLIVAQDICGKSITDMSWTPDGKILFITSLDSSIVVLTFEDNC",
    "response#3": "PDSQRLATGGLDGKIRIWSIPDILKFASNPNASTDKDILMKPLSTMSRHAGSVTTVKFSPDGKYLASGSDDRILLIWELEGGTTQPMFGAESTDIEHWNVRRRLVAHDNDIQDICWAPDSSIMVSVGLDRAIIIWNGSTFEKVKRFDVHQSHVKGVVFDPANKYFATASDDRTIKMFRYHKTGETSFSVEHVITEPFKGSPLTTYFRRLSWSPDGQHIAAPNAMNGPVSTVAIIERGTWESPVSLVGHDQPTEVASFNPRIFKRQKDDSTTDTIDGKKTGISDEVDCIVASSGQDKTLAVWSTSKARPLIVAQDICGKSITDMSWTPDGKILFITSLDSSIVVLTFEDNC"
  },
  {
    "reference": "MQKLACIILAAGKGSRMKSDLPKPLHAVAGQPMVQHVIDAANALNPEKLVVVVGKDMDALAQAVQPHDVAVQDVPNGTGGAVKPTRQYLDGFDGDVVILFGDSPLITPESIEKLVKARRAHPDTGLVFSAMLPDDPGMYGRMVVEKDDILDRIVEYKDCTEEQRKITLCNGGIMCADGRHLFGWLEKLSNDNVQGEYYVTHLPEIARADNLATRFIEIPEEDMAGINSREDLARVEKLLQNRLRKKAMENGVTLQDPDTVMFCFDTEIGHDVTIGANVVFGRGVTIESGAEILPFSHLEDCIIRSGAKIGPFARIRPGSDIGENAKIGNFVETKKAVIGKGAKASHLSYLGDVEIGAEANIGAGTITCNYDGFLKYKTVIGAGAFIGSNSSLIAPVTVGAGAYVGAGSAVSGTVPADGLAVTRAKTLIRAEWAKKFRDKKRKEKEQGV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein have MobA-like NTP transferase domains with specific sequence motifs that are crucial for its activity.2. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.3. The acetyl-CoA binding site should be located in a region of the protein that is accessible to the ligand.4. A protein that is capable of catalyzing reactions within the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, Nucleotide-sugar biosynthesis pathway with high efficiency and specificity.5. Mg(2+) is required for the proper folding and stability of the protein.6. The protein design must be able to perform UDP-N-acetylglucosamine diphosphorylase activity in order to contribute to cell morphogenesis, cell wall organization.7. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The designed protein sequence is ",
    "response#1": "VILAAGMGKRMHSALPKVLHPLAGRPLLSHVLDTARTLDPTRLVVVIGHGADAVRAAVAAPDVQFAVQEQQLGTGHALAQALPLIDPNVPTLVLYGDVPLTRASTLRRLTDAAGRNGYGILTVTLDVDDPTGYGRIIRNDAGEVQAIVEHKDANAEQLAIREVNTGVLAAPTQHLKRWLAALKNDNAQGEYYLTDIVAMAVADNVPVVSAQPADASEVSGVNNKLQLAELERVYQRQQAEQLMLAGVTVQDPGVTYIDADVTIGMDTVILPNTSLRGRTVIGEGCEVGPNSVIQDSRIGDGCRVIASMLEGATLEAGVDVGPFSHLRPGAYLETGVHIGNYVEVKESRLGRGVLAGHFSYLGDASIGTGTNIGAGTITCNFDGTDKHPTKIGANAFIGCDTMLVAPVTVGEGAATGAGSVVTKDVPGHLLAVKAPKRIKQGWKRPVKQKK",
    "response#2": "VILAAGKGTRMNSDLPKVLHQIANAPMLVHTMQAGAALSPAHTVVVAGHGFDAVKAAALDFDPDAKVVLQEEQNGTGHATNMARDALSDFDGDVIVLFGDTPFVRADTLEKMIVARKDNAVVVLGFEADPQDFGRIVRENGTVTGIVEAKDANAEQLAINEINTGILAVSAPLLKQWLAQLDNDNAQGEYYLTDVIAMAHADGVAIHTAQPAAAWEVEGVNDRVQLAALERYYQRQQAEALMKAGVTVQDPGVTYIDADVTIGMDTVILPNTSLRGRTVIGEGCEVGPNSVIQDSRIGDGCRVIASMLEGATLEAGVDVGPFSHLRPGAYLETGVHIGNYVEVKESRLGRGVLAGHFSYLGDASIGTGTNIGAGTITCNFDGTDKHPTKIGANAFIGCDTMLVAPVTVGEGAATGAGSVVTKDVPGHLLAVRADLAEAEAAFQARMRAQMMADGVTLIAPETVFFAHDTKIERDVVIEPNVVFGPGVVIEEDVVIHAHSHVEGAHLKRGAHAGPFARLRPGAELGEGSKVGNFVEIKKSQLAEGAKVSHLTYIGDASIGANANIGAGTITCNYDGYDKHRTVIGENAFIGSNTCLVAPVTVGAGAFTATGTIVTEDVPADALALARTPQRNIDDWKRPVKLKMKQ",
    "response#3": "VVVLAAGQGSRMKSDLPKVLHPIAGKSMLAHVLDSARSVKANALHVVVGHGSDKVKSHFCDDNDIQWALQAEQKGTGHAVKMALDNLAPSGVTLILYGDVPLIQANTLQALIDLVTSEVPNSLGRIVRDEIILAAGQGSRMNSDLPKVLHKIAGTSLLAHAMGSADALMPQRCVVVAGHEAELVRAEALSLGVVDVVLQGEQLGTGHAVLQARDALSDFDGDALVLYGDTPFISPETLEKLAEARKDHAVVVLGFRPKDPARYGRLVMSGDKLVAIVEEKDATPEQREIREIYSGIMAVPTRHLKRWLGRLQNKNAQGEYYLTDIVGLAVADGVPVVAYQAEDAAETLGVNSPLQLAELERRHQRQAANRLMEQLAQAEAIFQDRARADLMDLGVTLQQPETVMLSFDTIIGRDTTIEPNVYFGPEVTIESGTRIRAFSHLEGCHVSRGATVGPYARLRPGAELAENTHVGNFVEIKNAEVGEGTKVNHLSYVGDATVGKGTNIGAGTITCNYDGVMKHRTEIGDGAFIGSNTMLVAPVKIGNQAMTGSGSVITKDVPDTALALARAEQVVIANWRRPQKR"
  },
  {
    "reference": "MDEQARFYTAQQVKSGEQKAAKDCQVEMYALMERAGLAVFQTIKRQFPSVRRVLVCCGGGNNGGDGYVVARLLLEAGVNVELWSAVDIEKLTGDAKSAYQAYIGAGGRVEKPALAIVSDVELIVDAILGTGLTGEVRVAQRSIIELINAHSAKVISVDIPSGLCADTGSVLGVAIEAFYTVTFIGMKQGLVTGQARNHTGKFVYDGLGVSETFHRQNVPATLAIDSRCWQSSLPKRKPASHKSTHGKVALIGGEQGFSGAIILSGNASARSGAGLTAVLTNEVSIAPMLNHCPEVMVSNWQSMAFVAKRVDWSDAIALGPGLSTSNHARSLFELVRRYDKAKVLDADALTLLAENPFKDDNRILTPHPGEAARLLACSIKEVESNRFQAVTQLQQIYGGVIVLKGAGTLVHDGQTIYVCTAGNAGMGSGGMGDVLTGVIVSLLAQGMSLSDAALTGVQLHSVAADELATEFGSRGLLASDLIPAIRRLMNQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The (6S)-NADPHX, K(+), AMP binding site should be stable and able to withstand changes in environmental conditions.2. The designed protein must have at least one YjeF N-terminal domain. The designed protein sequence is ",
    "response#1": "LSDQDDLKLKRFAEDVYKNTRGHLALFAGSIAFSGAARLSARAAFHSRCGLVTLCCDRDVASVAASETPSIIVEAIEQQAVLKGSQLVRTYQAVAAGPGWGDGREEQVLELLQSGLPTVLDADAIGAFAKLVGDHRIGSTAHGPLVLTPHPGELHRMLEALHMPLLAQETGPSGTPESFIDSLKRVALALEIVLVYKSHVVWIIDGRSGGSLPIVVDGMNNALGVAGSGDVLTGIIGSLLAQGYDVFEAAHTGVLVHQGAGLLARNESGWFDAETLITHIGNVCRSAEVGRV",
    "response#2": "GKGDLLSFNQNRSLKSHKGDSGRVLVIGGNETFSGAPALVSLAAYRTGSDLVMTVVPSKVAASVRAYSPNLIVKDYEGKNFNEMGLELTKKLIEWPTAVAIGPGLGREEETNKNVIDFLSIVANKNIPTVIDADALKAIKDDLAILKKGNFLLTPHLGEYNILSNQNLNANDEMNKKIKNIFEFAKKIEATILLKGHYDIISNGRRLKINKTGTPAMTVGGTGDVLTGITASLMGMKIDPMKSACIGAFINGIAGELATNDLNGNHLLASDLLDYLPKAFHS",
    "response#3": "LLIAGSYGMGGAAVLAARACMRSGIGLLTVHSPICNHQLLQTIVPEAMVQDDVHERFFAEPTDLDNYQAMAIGPGLSQEEITAQAALDQVSNCFIPLVLDADALNIFSAYRSYLNRLPKHSILTPHVKELERLVGRCSNSFEHLSKAKELAGELQVYIILKGAWTTVITPEGNCYFNPTGNPGMATGGSGDVLTGILLSLLAQGYTQEEACRLGVYIHGLAGDIACMRMGEISMTAGDIVNALR"
  },
  {
    "reference": "MQNTHSSSPAGATAAQAPEASTHFGFQTVAETEKAGKVAEVFHSVAQRYDVMNDLMSLGLHRAWKVFTVGRADVRPGMKVLDIAGGTGDLARAFAKKAGASGEVWLTDINDSMLRVGRDRLADRGMLLPTAVCDAEKLPFPSAYFDRVSVAFGLRNMTHKDQALAEMRRVLKPGGKLLVLEFSKVAKPLSPVYDWYSFNILPWLGQKVAGDQESYRYLAESIRMHPDQDTLAGMLEAAGLSRVRYFNLSAGVVALHEGVNLG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit demethylmenaquinone methyltransferase activity.2. The protein have enhanced a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "MSILSGR",
    "response#2": "MILLHVFLAL",
    "response#3": "MAEIRIRNTNERILG"
  },
  {
    "reference": "MNTTDAKEYLSKREIPQLFESMLTGLMYHRPEDPVLFLEGCLQKVRELGGPERVSWDTFITPERRPLPPLSVGQGRKVASRSESGPGPGIGPYRRYERLPPIQAQYSIESDSDMTETSGLIQEYDVFGPSKPRPQIIFVIGGPGSGKGTQTSKMARHYNYESISVGEILRNQLLHHAPSDRKWELIAQIIANGELAPQDTTIEELKHQFIKRQGAEGFIVDGFPREISQAFTFEEQIGSPDLVVLLACSNQQLRQRLEKRAALQGRPDDNAHAIERRLETFKQNITLIAKYYQERGLIVRIDAERDEDEIFADIRAVVLERLFPNGDTGITDRDALQPDDGEVERPEAPGEGQGLLFTEQIAVTDKLKDHKIFFVVGGPGSGKGTQCERVAAKYGYTHLSTGDLLRSEVDSGSERGKQLSAIMQKAELVPLDMVVDMLKDAMIAKADVSKGFLIDGYPREVKQGQEFEKKIGAPDLVLYIDAKEDTMVQRLLKRGETSGRADDNEATMKKRLEMYYKATEPVITFYEGRGIVWKINAEAPVDEIFTSVVTAIESLK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a high specificity for AMP binding, with minimal off-target effects.2. For general function, the protein need meet that Also displays broad nucleoside diphosphate kinase activity3. The designed protein should be optimized for ATP binding activity in order to improve phosphorylation, ATP metabolic process. The designed protein sequence is ",
    "response#1": "RKYFYNDVLVATRHAVEDKKTRLQIDINIPELNPEMDVYRIGTLMELVRELSLSFADDGKRVKVCVQGSMGQGAFAGIPLQLAGTRKILEIMDWGEYGAKGTFINFGAVGASEVDKEDDMFILIAPQNAVGNCIIDDMKAMTDAAGDRPVILVNPRLKDMPGSSGVMQTMGRDMRLKYAASFETCYSFRLLFYAGSFYPIMGALRMAYPNKYEIYRRVDEPNGQERYVLLEEFVEKPTPDEIV",
    "response#2": "RKYFYNDVLVATRHAVEDKKTRLQIDINIPELNPEMDVYRIGTLMELVRELSLSFADDGKRVKVCVQGSMGQGAFAGIPLQLAGTRKILEIMDWGEYGAKGTFINFGAVGASEVDKEDDMFILIAPQNAVGNCIIDDMKAMTDAAGDRPVILVNPRLKDMPGSSGVMQTMGRDMRLKYAASFETCYSFRLLFYAGSFYPIMGALRMAYPNKYEIYRRVDEPNGQERYVLLEEFVEKPTPDEIC",
    "response#3": "RKYFYNDVLVATRHAVEDKKTRLQIDINIPELNPEMDVYRIGTLMELVRELSLSFADDGKRVKVCVQGSMGQGAFAGIPLQLAGTRKILEIMDWGEYGAKGTFINFGAVGASEVDKEDDMFILIAPQNAVGNCIIDDMKAMTDAAGDRPVILVNPRLKDMPGSSGVMQTMGRDMRLKYAASFETCYSFRLLFYAGSFYPIMGALRMAYPNKYEIYRRVDEPNGQERYVLLEEFVEKPTPDEIL"
  },
  {
    "reference": "MTTVAIITAAGRGTRAGGDLPKQWQMLAGRPVLAHTLEAFAGFRRVLTIHPEDRAWAQTLGGDIELVEGGATRAASVHNALRALAGSGVTRVLIHDGARPLVPGSVIANVLTALDDHAGAAPALPISDALWRGDAGLVSGTQDRSGLFRAQTPQGFRYDAILAAHDAHPGNAADDVEVARAAGLTVAIVEGHEDNLKLTYPQDFQRAEAILKGRRMDIRLGNGYDVHAFTDGDHVWLCGVKLPHGKALLGHSDADVGMHALTDAIYGALAEGDIGRHFPPSDPQWKGADSAIFLRHAMERVAARGFSLANCDVTLVCERPKIGPHAEAMRAALAKIMGVEMDRISVKATTSERLGFTGREEGIAALATAALVRA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should interact with enzymes and substrates within the isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6 pathway to enhance or regulate its activity.2. The protein should have a high affinity for 4-CDP-2-C-methyl-D-erythritol 2-phosphate.3. For general function, the protein need meet that Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D- erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C- methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).4. A protein with 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity, metal ion binding functions is required to enable terpenoid biosynthetic process. The designed protein sequence is ",
    "response#1": "MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDDRYKGISSIKLLEEVGKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADALKIDVDRVNVKATTEEGLGFTGSGEGISSQAICSLIYTRKRPQRNIRSGIGTDVHAVDGTQPLWLAGLHWPGEAGLAGHSDGDAVSHAVCDALLSAAGLGDIGGIFGTDDPELDGAHGDVFLRRTSELVRGAGYGIVNVAVQVMAVRPKLSPRRAEAERVLSAAVGAPVSLAGTTTDGLGFTGRGDGVAAVATALVYQ",
    "response#2": "MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDDRYKGISSIKLLEEVGKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADALKIDVDRVNVKATTEEGLGFTGSGEGISSQAICSLL",
    "response#3": "MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDDRYKGISSIKLLEEVGKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADALKIDVDRVNVKATTEEGLGFTGSGEGISSQAICSPRVGLGYDVHAFAEDRKLILCGIEVPHEKGLAGHSDADVGIHTLCDAIYGALAEGDIGRHFPPSENKWKDMDSARFLVHAGELIRKKGGYLVNADITIICERPKIGPHAQAMRERLAELLNVSVSRISVKATTSERLGFTGREEGIAATAAVSIMWRIGD"
  },
  {
    "reference": "MSKIALATGNHHKVLELKQLFQRSLINNPPLILTPQDCGIISFSPIEDGLTFSENASIKSNALFQVTGIPSLADDSGISVDALEGRPGIYSARYGGEGITDQQRAMLLLKEMQGIKNRSAHFDCVLSFTTESGTVSFAGRVSGEIAQVYDESGNGFGYDPIFYYPEWDKCFSEISPEQKNTISHRAIALQKFISYLAESTGPPVSRL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a high affinity for substrate, Mg(2+).2. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in dITP + H2O = dIMP + diphosphate + H(+) are properly positioned for optimal catalysis.3. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.4. The protein must bind to Mg(2+) in order to perform its enzymatic function.5. A protein with ribonucleoside triphosphate phosphatase activity, nucleotide binding, dITP diphosphatase activity, metal ion binding, XTP diphosphatase activity functions is required to enable nucleotide metabolic process, purine nucleoside triphosphate catabolic process. The designed protein sequence is ",
    "response#1": "MKIVFATNNRHKLEEVKALVPASIELLTMDQIGCREDIPETANTIEGNAIQKANYVKEHYGYDCFADDTGLEVDALNGEPGVYSARYAGEQRDANDNMNKLLQTLQGKENRKARFRTVIALNLNGKQHLFEGVINGTITEEKRGTDGFGYDPIFLPDDYEVTFAQMSLEEKNKISHRGLAVQKLVTFLKENQK",
    "response#2": "MNNEILIATSNKGKVKEFRELFRSKYNIKSLNDFNPIKPIEDGNSFEENAIKKATFVSSNLNLPAIGDDSGLCVDALNNAPGIYSARYSGSASTYKDNINKLLKELGDIENRKAYFVCVLCWVNSANDPMPIISQGKFEGVITTEKNGTGGFGYDPLFYLPDFEKTFAELSSKEKNNISHRGKAFEQFLKILNND",
    "response#3": "MEIILATHNQGKVKELQALLGDKFNIKTLIDIDIPDVKETGNTFEENALIKARAVYNATKLPALADDSGLVVEALKGAPGIFSARYAGENATDKQNNEKLLNELKGVTDRRAAFRTVIAFVHPNGKILTAKGECEGIIAFEAKGENGFGYDPVFYLPEQNKTFAQMTMEEKNQISHRGKALKALFQYLEKE"
  },
  {
    "reference": "MAIDRTVLVSPDAMGQIDKAAADSGIDSFGLMRSAGAHVAAVVLARFPQAQRAVVLCGPGNNGGDGHVAGMLLQRSGMEVQRFGLEPKPGSDAARARAQFPGDICPLSDWRPRAGDVIVDALFGAGLDRPVSGDVEALIERASRAKTPVVAVDLPSGLSGRTGEPTGACFQALHTVTFAALKPGHLLMPGRELCGEMHLCDIGIPARLIASDDTVWRNHRGLYAAHLPGSAASQHKYTRGHLVVFSGPLISSGAARLSAMAGLRCGAGLVTVAAQPSAVMVQATHLTAIMQRAIRDAAELADWLGDSRLSGFVIGPGFGDLEKARAYTSLLAEAGQPVVLDADGLTAFAGKTEELAELFAGEARLVLTPHEGEFRRLFADLADDVTLSKIDRARAAARAQNAVVVYKGADSVIAAPDGRAAVNADAPAWLATAGSGDVLAGIIGGLLARGMPVFEAACAGVAMHGEAARRAGSGLTAEDLPTHIPAL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.2. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.. The designed protein sequence is ",
    "response#1": "VGIAAGSARYPGAPVLAVTGALRGGAGAVRYAGPAAEAVTARHPETLVSPGLPSEAGRVQAWVVGPGLGDGDEARAALDDVLATDLPLLMDADALSLAGPDRISARSAPTLLTPHAGEAARLLGADRADVEARRLDAVRELADRLGATVLLKGSTTLIADPGGTSPVRVNATGTSWLATAGAGDVLSGLAGSLLATGLPPRDAASAAAHLHGLAARRAG",
    "response#2": "GIVSGTMPGASLLAAVAAQRAGAGYVKLLGRAADPRTPPDLVVDTKPLSEALADPRMAAVLVGPGLGRDGAAKAALGEGLRRAQALVLDADALVLLAPNMLAREVPMLATPHDGELETLCRTFGIIAEGRQPRVLALAKMTGMVVLAKGPDTVIAAPDGRLAIGTPAPTWLSVAGTGDVLAGIAASRMANGADPFTAACEAVWLHGEAARRSGPAFTASQLAERVSEV",
    "response#3": "DSHKYIRGLLGIVSGTMPGASLLAAVAAQRAGAGYVKLLGRAADPRTPPDLVVDTKPLSEALADPRMAAVLVGPGLGRDGAAKAALGEGLRRAQALVLDADALVLLAPNMLAREVPMLATPHDGELETLCRTFGIIAEGRQPRVLALAKMTGMVVLAKGPDTVIAAPDGRLAIGTPAPTWLSVAGTGDVLAGIAASRMANGADPFTAACEAVWLHGEAARRSGPAFTASQLAERVSEAL"
  },
  {
    "reference": "MKVDLTIEYRGKLITPNQIKLLIALHKTKSQNEAAKLLNIKPSSFNIQLKRLENKLGVKLYYSSPNGTVLTDAGLEILETYNSYSKRLKNHFFTVSGFVSGEIAKILFGNPIITSFDNALKLLKMGFVDVLGVDDSYWIYRLGDERFLKSEIGSCDFNIFLIAYDNFVMVSKKEFNYKNLVGIRFSSQRIVYNILKKEGIKFKVKVRVKNPFRAIELVNEGYSLFLNESLLRYIDGDFNVIYPNFYEKTVHAINFISFGKDIEIDKKEIKKIKKLGFKI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The H-T-H motif domain within the designed protein should be capable of recognizing and binding to a range of DNA sequences. The designed protein sequence is ",
    "response#1": "VRFPRGATIFDEGEPGDRLYIITSGKVKLARHAPDGRENLLTIMGPSDMFGELSIFDPGPRTSSAVCVTEVHAATMNSDMLRNWVL",
    "response#2": "IFQGVDPTAVNNLIQDMETVRFPRGATIFDEGEPGDRLYIITSGKVKLARHAPDGRENLLTIMGPSDMFGELSIFDPGPRTSSAVCVTEVHAATMNSDMLRNWVADHPAIAEQLLRVLARRR",
    "response#3": "IFQGVDPTAVNNLIQDMETVRFPRGATIFDEGEPGDRLYIITSGKVKLARHAPDGRENLLTIMGPSDMFGELSIFDPGPRTSSAVCVTEVHAATMNSDMLRNWVADHPAIAEQLLRVLARV"
  },
  {
    "reference": "MNPALPRHIAVLKGGPSEEREISLRTAKAVEDALSSLGYEISSIDVTTEKFEIPRDAEICFLCIHGSFGEDGQIQRLLMRRGIPFTGSDASSSEKAFDKAWSKTLFIKGGIPTPPFCVVGDGKKIPFDPPYVIKPSRQGSSIGIEFVYDIKELDQAIKKSTQYDHVVLAEALITGKELTVGILDGKALPVVEIKPKEGFYDYHHKYTKGASTYFCPAPLTVDQTSAVQKTALDAFNVLGCSVYGRVDIILSDNGIPWVLEINTIPGMTETSLFPMAAKASGMNFAQLCEKILEISYLRWKAHG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The presence of Mg(2+) is necessary for the protein to function in the desired environment.2. A protein that is capable of catalyzing reactions within the peptidoglycan biosynthesis pathway with high efficiency and specificity.3. We are looking for a protein with broad substrate specificity for ATP + 2 D-alanine = ADP + D-alanyl-D-alanine + H(+) +   phosphate.4. The designed protein should have metal ion binding, D-alanine-D-alanine ligase activity within cytoplasm to regulate peptidoglycan biosynthetic process, regulation of cell shape. The designed protein sequence is ",
    "response#1": "IWRADGLPTPDWRLVSSSAETAQAFQELGAPMIVKPSREGSTIGLTKVTSLGQCEQAYRLAAQHDPEVLCEQFIDGDETTCPILGQGTEARALPVIRIEAPQGNYDYQNKYFTDVTQYHCPSGLPEAEEREIQRIVVQAYRTLGCRGWARADIMIRASDRKPFLLEINTSPGMTGHSLVPMSANASGISYPDLCLRIIAITGTNGKSTTTALLAHLLEQMGAKE",
    "response#2": "LWHGCGLSTAPFAVLGADTDWQQIVNTLGLPLIVKPVHEGSSIGMSKVNTLDELPKAYEVAAGCGDVVMAEKWITGREFTIVIIDDEAYPVIRLQPADISNFYDYEAKYNRNDTQYHIPCGLSAEEEAHLQALSLAAFKAVDAKGWGRIDAMQDEEGNFWLLEINTVPGMTSHSLVPMAAKARGMNFEQLCWHIAP",
    "response#3": "FEQAGLPQVKYKAVTRAQIWSNPCVFPKLCDEIEASLGYPCFVKPANLGSSVGISKVRSRQELEDALDNAANYDRRIIIEAGVAAREVECAVLGNDQPQASTVGEITFNSDFYDYETKYTAGKADLLIPAIIPDDISRQIQNMALQAFAAVDAAGLARVDFFYVEATGEVLINEINTLPGFTATSMYPQLWAHSGIPFPELVDKLVIG"
  },
  {
    "reference": "MARVLNVEIPNKKRAVISLTYIFGIGKTLASQILKDANVDENKKVESLSEEELTRIREEAKKYVTEGDLRREINLNIKRLMEIKSYRGIRHRKGLPVRGQCTQKNARTRKGPRKTIAGKKGK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein can be targeted to ribosome and perform rRNA binding, tRNA binding.2. The protein must exhibit the following characteristics: Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA; In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement.3. The designed protein's functionality will benefit from Basic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#3": "MTAIAGQKPLITKARKF"
  },
  {
    "reference": "MPRFALTIIRHGETRLNKEKIIQGQGVDAPLSETGFRQAAATGQFLSNVHFTHAFSSDLTRTKQFDFICQLILGKAGQRENFLPGAPDNCLESSLAEVFPVGKHGSLGTDPKHGALGLTASILVVSHGAYMRSLFGYFLSDLRCSLPATIDKFELSSITPNTGISVFIIDCEEARKPTVQCVCMNLQEHLNKGVEKH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be involved in glycolytic process, intestinal epithelial cell development, cellular response to hypoxia, mitophagy, positive regulation of DNA repair by localizing to mitochondrial outer membrane, cytosol, nucleus and exhibiting bisphosphoglycerate 2-phosphatase activity, fructose-2,6-bisphosphate 2-phosphatase activity. The designed protein sequence is ",
    "response#1": "VGVILSGGQAPGGHNVVCGLYDALKATNKENVLYGFKGGPSGLTDDKYIVFDDEYIDQFRNTGGFDIIGSGRTKLETEEQFAQAAEVCKKHGINAIVMIGGDDSNTNAAVLAEYFAAHNTGVQVVGCPKTIDGDLKNEDIECSFGFDTATKTYSELIGNIERDANSAKKYWHFIKVMGRSASHVALECALETQPNICLIGEEVAAKKMSLSQIADQIADSVAARAANGDNFGVAIIPEGIVEFVPEFSVLIAEINELLAGEKADKFNALETWTEKYEFIKNGLTAESFAVFDILPEGIQKQLFLERDPHGNVQVSLIESEQLFAELVKDKLEERRKAGTYNGSFKTQHHFFGYEGRCAFPSNFDADYCYSLGYNAAMGVIKGRWAG",
    "response#2": "VGVILSGGQAPGGHNVVCGLYDALKATNKENVLYGFKGGPSGLTDDKYIVFDDEYIDQFRNTGGFDIIGSGRTKLETEEQFAQAAEVCKKHGINAIVMIGGDDSNTNAAVLAEYFAAHNTGVQVVGCPKTIDGDLKNEDIECSFGFDTATKTYSELIGNIERDANSAKKYWHFIKVMGRSASHVALECALETQPNICLIGEEVAAKKMSLSQIADQIADSVAARAANGDNFGVAIIPEGIVEFVPEFSVLIAEINELLAGEKADKFNALETWTEKYEFIKNGLTAESFAVFDILPEGIQKQLFLERDPHGNVQVSLIESEQLFAELVKDKLEERRKAGTYNGSFKTQHHFFGYEGRCAFPSNFDADYCYSLGYNAADGQMSGLTGTAASVTKIGVLTGGGDCPGLNAVIRGVVRKGVGLYGHSFVGFRDGWRGPLENDTMPLDIAAVRGILPRGGTILGSSRTNPFASADGPARVRETMRRTGVDALIAIGGEDTLGAAARLHAEGIPVIGVPKTIDNDLSATDYTFGFDTAVNIATEAIDRLHTTAESHHRVLVVEVMGRHAGWIALHSGMAAGANVILIPERPFDIDKVCAFVEQRFATSYAPIIVVAEGATPIAGTMMVKEGELDAFGHVRLQGISALLETEIRGRTGRETRATVLGHVQRGGTPTAFDRWLATRFGLHAVDLVHEGKSGLISSVEGLTKEPVDWIAGGIPLTQLMDVERRVSKSHPQWI",
    "response#3": "VGVILSGGQAPGGHNVVCGLYDALKATNKENVLYGFKGGPSGLTDDKYIVFDDEYIDQFRNTGGFDIIGSGRTKLETEEQFAQAAEVCKKHGINAIVMIGGDDSNTNAAVLAEYFAAHNTGVQVVGCPKTIDGDLKNEDIECSFGFDTATKTYSELIGNIERDANSAKKYWHFIKVMGRSASHVALECALETQPNICLIGEEVAAKKMSLSQIADQIADSVAARAANGDNFGVAIIPEGIVEFVPEFSVLIAEINELLAGEKADKFNALETWTEKYEFIKNGLTAESFAVFDILPEGIQKQLFLERDPHGNVQVSLIESEQLFAELVKDKLEERRKAGTYNGSFKTQHHFFGYEGRCAFPSNFDADYCYSLGYNAAYLISEGKTGYMSSVRNTTALSFTSFVTNDDKLFEETFNFYTKLGFHATRSYVKDNRSDFELTGISTDSIKEIWLESFPLSEVVETSAGRELRKPLQESVGYQSEALLGYSPYQSDGVVIKLRE"
  },
  {
    "reference": "MHLKAFGKVNLYLDVISRRKDNYHNILTLFQSINEHDDIFIEFSESEIFESEPPLNISWDNNIIKKSIETFKKETGFDNFNLKIKLIKNLPMGGGLGGGSADAAAVLNFLSKNFKISEKDLFEISCKIGSDVPFLLKGGTAIGKGKGEILEFLEPLKLNIQIYPMNYNIDTKKMYEKLDQNWKSINHFGDPYNLYEALKNNDIITAKQNAFNVFEQVAFKEYPKLKQKKEDMEKDEGIIFALMSGSGSTLYKVIYAN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that can modulate the overall flux of metabolites through the Isoprenoid biosynthesis, isopentenyl diphosphate biosynthesis via DXP pathway pathway, enhancing or suppressing its activity as needed.2. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.3. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol..4. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.5. The designed protein must possess ATP binding to support isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway, terpenoid biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "MSFRIGHGYDVHKFTSAKQNIIIGGVEIAYHLGLEAHSDGDVLIHALCDAILGALGLGDIGKHFLDTDNQFKNIDSKFFLAEIKKMLDKKQYSISNIDCTIIAQAPKMLPHIEKMRACLANILEIQISQINIKATTTERLGFIGREEGIATHVVCLLETV",
    "response#2": "MSFRIGHGYDVHKFTSAKQNIIIGGVEIAYHLGLEAHSDGDVLIHALCDAILGALGLGDIGKHFLDTDNQFKNIDSKFFLAEIKKMLDKKQYSISNIDCTIIAQAPKMLPHIEKMRACLANILEIQISQINIKATTTERLGFIGREEGIATHVVCLLE",
    "response#3": "MSFRIGHGYDVHKFTSAKQNIIIGGVEIAYHLGLEAHSDGDVLIHALCDAILGALGLGDIGKHFLDTDNQFKNIDSKFFLAEIKKMLDKKQYSISNIDCTIIAQAPKMLPHIEKMRACLANILEIQISQINIKATTTERLGFIGREEGIATHVVCLLS"
  },
  {
    "reference": "MVDINLECVHFIEPKNTRSTEQQQQNVKNAYLEAVRILLVLLENVLSQPNNTKFRTIRLDNKAIKEKLLSLPGCEKLLYAIGFRHTPSSNSYTLPGEVSLQQIQKYYDVLRERRTAWLSGAMPKSPAHPKAACSTPSPLFIKTSVPYRQRITFPRVLRTSNRFLQSLELYSDAVMQYEDELLLATGRSLIPVDDLTSKASEKMMAMQDLIASGECSEKEPCIRDLLLVELTNWFNTQFFEWVNNIQCQVCGSEESKLRRTQTEGDVRVEVNVCCGQESKFYRYNDISQLLVSRKGRCGEYANCFTFLCRCLDYDARLVHSHFDHVWTEVFSETQMRWLHVDPSDNVVDSPLMYQHGWKRGIDYVFAYSRDDAQDVTWRYTNNHQEILKLRKLCDEKELIQTLNAIREKRQQNCSAERKNFLSQRNMFEVIGLTLERKPTENELKGRSSGSLSWRQSRGEHTFTNIFVFSPNEAELKKCQLNLRYSCATDTYERYTKDDGQITLLNTYKTWQSAQFSSKNIYRKVERDWKMAYLARLEDTDCAEIIWKFDLTKTNLKVKSYKLVFETKTFGEGKIDVSVQTNDGKTFIENSSEFQVVAKLMGGKGDVAWQHTQLFRQSLNSREYPFDLQVELH",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein have PAW domains with specific sequence motifs that are crucial for its activity.2. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects.3. For general function, the protein need meet that Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp; Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins .4. The protein must bind to Zn(2+) in order to perform its enzymatic function.5. The active site, Nucleophile of the protein must be engineered to enable effective catalysis of Hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl)asparagine   residue in which the glucosamine residue may be further glycosylated,   to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a   peptide containing an aspartate residue..6. A protein that can perform metal ion binding, carbohydrate binding, peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity in cytosol, cytoplasm to support glycoprotein catabolic process, response to stress, positive regulation of BMP signaling pathway, protein deglycosylation. The designed protein sequence is ",
    "response#1": "MFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGPEANVGPYGSGDSAPLQEAMAVLQHHDAVSGTARQNVVNDYARQARAYWSGYYTTRPYQKILARQFEHQLRSAEILFTLVSNYIRQMGRQGEFGASEKKLEKSYEQLIYARRNLGLFQHHDAITGTSKSSVMQDYGTKLFTSLYHCIRLQEAALTPILVFGFAIYAFYLRHLTAGKYFPDPDTFYHFEIYKLVLKEGLPRYYPMSDAPFGSLIGEPLGLYLLPAAFYKVVSLFGYNELQAFLLWPPFVGFLGVIAVYLLGRKVLNEWTGLWGAVVLTVSTANFSRTFSGNARGDGPFMALFIFASVAMLYYLKESNKTRKIIYGTLFVLLTVISLGAWNGSPFGLMVLLGFASLQTIILFIFGKLEELKKFVKEFYPAYLAILAFGYALTFPGIVKIGGFIRFAFEVFLGLIFLLVIMLYGGRYLNYSDKKHRFLVVTIIVLLGFGGAYAYVGPKLFRLMGGAYQSTQVYETVQELAKTTIGDVKAYYGVESGNGLIFFLSIPGLLILLTKYLYDLFKKAKSDNETLFALVFYTMSLYLLYLAVRFLFLASYAVALFFGIFIGFSMDVIEKMKENIGIKAALGIVLSLMILVIPFVHLT",
    "response#2": "IVMAAGRGTRMKSRIPKVLQRLAGRPLLLHVLDQAAPLAPRQVVVVTGHGAMEVEAACQGETRAGGSFDLQCVRQEPQLGTGHAVQQALPRLAGDGTVLVLSGDVPLTQAGTLAALVAASGGAALTALTLDPSGYGRLVREGGAVGAIVEHKDASEAQRAIAEVYSGIMAAPTALLRGWLARLDNRNAQGEYYLTDVIAMAAGEGVAVVPALCDEAEGVQVGEGALVGPFARLRPGAQLGREVHIGNFVEVKDPGSGVRLADPARLDVRDDVRSGVRAALSCGADVEIDAGCIFAGQVEIGEGARIGAYCHIANARIGAGAVIHPYTHIDGEAAGVQVGEGALVGPFARLRPGAQLGREVHIGNFVEVKNSQLADGAKANHLAYLGDATVGERVNYGAGSITANYDGANKHRTVIEADVHVGSNCVLVAPVTIGAGGTVGGGSTVTKNAPPGALTVARGRKTRKIGG",
    "response#3": "IVMAAGRGTRMKSRIPKVLQRLAGRPLLLHVLDQAAPLAPRQVVVVTGHGAMEVEAACQGETRAGGSFDLQCVRQEPQLGTGHAVQQALPRLAGDGTVLVLSGDVPLTQAGTLAALVAASGGAALTLLTVELADPTGYGRILRDAEGNALAIVEQKDASPEQLAITEVNTGILAAPAERLREWLSGLANDNAQGEYYLTDIIALAVRDGLAVATRQPATAWEVEGINDRKQLAELERIHQLQAARALLERA"
  },
  {
    "reference": "MKLIKTKGIVLKETNFEESSKILTVLTSDFGKIQVLSKNCRRLLSVLSACSQPLMFCEFVIRKTKDIYSISSASVIESFFELSQDVNLAIYSGYLIELVDSFLEFEQKNEDVLRLLLNSLYLLKKGKDPEVVSRIFEIKILVYTGFFLQFTQCVKCQRKDITRAFFSFKDGGLTCEKCKEENDIEIEIEVVKSILVIVATNLKKLNKISLERSLNNKIKTITLPYIKMVLQKDIKILDFFRFIQ",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should aim to mimic or enhance the function of natural proteins involved in DNA recombination, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "MLQKTVGIVLHVLKYNDTSNIVEMYTELSGRASFLVTVPRSKKATVKSVLFQPLALIEFEADYRPNTSLFRIKEAKSFSPV",
    "response#2": "MEWSGEASVMALQRHGESAVILTVLSRETGLIRGLVPGGASAKRAAMLQPGNRVSLRWRARLEEQLGTFL",
    "response#3": "MLQKTVGIVLHVLKYNDTSNIVEMYTELSGRASFLVTVPRSKKATVKSVLFQPLALIEFEADYRPNTSLFRIKEAKSFSPM"
  },
  {
    "reference": "MGGKWSKSSVIGWPAVRERMKRAEPEPAAVGVGAASRDLEKYGAITSSNTSQTNPDVAWLEAQEGEEVGFPVRPQVPLRPMTYKAALDISHFLKEKGGLEGLIYSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPDKVEEANEGENNCLLHPISQHGIDDPEREVLQWKFDSRLAFHHMAREMHPEYYKD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK12. The designed protein should have SH3 domain binding, GTP binding within host cell Golgi membrane, membrane, host cell plasma membrane to regulate suppression by virus of host autophagy.3. The protein's functional design necessitates the inclusion of a Dileucine internalization motif; necessary for CD4 internalization, Diacidic; necessary for CD4 internalization motif. The designed protein sequence is ",
    "response#1": "MEPVDPRLEPWNHPGSQPKTPCTKCYCKQCSYHCELCFLKKGLGISYGRKKRRQRRSAPPSSEDHQNPISEQPLSQTRRGDHPTGSKEQKKKVESKTETDPFD",
    "response#2": "MGGKWSKSSIVGWPAIRERMXRAEPAAEGVGAVSRDLEKHGAITSSNTAATNAACAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLVYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRYPLTFGWCFKLVPVEPEKVEEANEGENNSLLHPMSLHGMEDPEKEVLQWKFDSRLAFHHMARELHPEYYKDC",
    "response#3": "MGNKWSKGWPAVRERIRRTDPAAEGVGAASRDLEKHGAITSSNTAATNADCAWLEAQEEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQKRQDILDLWVYNTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPLDPDEVEKANEGENNCLLHPMSQHGVDDPEREVLKWKFDSRLAFHHVARELHPEYYKN"
  },
  {
    "reference": "MAAAAAEFRGQARLPRFAAPRRYELRLRPDLAACVFSGEASVAVDVSAPTRFLVLNAADLAVDRASIRFQGLAPAEVSVFEEDEILVLEFAGELPLGEGVLAMRFNGTLNDQMRGFYRSKYEYKGETKNMAVTQFESVDARRCFPCWDEPSFKAKFKLTLEVPSELVALSNMPIVNEKIAGPIKTVEYEESPVMSTYLVAIVVGLFDYIEGVTSEGNKVRVYTQVGKSNQGKFALDVGVKSLNLYKEFFDTPYPLPKLDMVAIPDFTNGAMENYGLVTYREIYLLFDEQSSSASTKQNVAITVAHELAHQWFGNLVTMEWWTHLWLNEGFATWMSYLAVDSFFPEWNIWTQFLDSTTSALKLDSLAESHPIEVEIHHASEIDSIFDSISYDKGASVIRMLQSYLGAERFQKALASYIKKYAYSNAKTEDLWAVLEEVSGEPVKNLMTTWTKKQGYPVIGVKLKGHDVELEQDQFLLDGSSDSGMWIVPITLGCNSHDMQKRFLLKHKFSDIKGINSQYDDQDRQNSGNFWIKLNIDETGFYRVKYDDELTTALRNALQMKKLSLMDKIGIVEDAHALSIAGKQTLSSLLHLLYACRDEDDFSVLSHINSVTSSVAKISIDATPELAGEIKQLFIKLLLPTAEKLGWDPKNSESHLDAMLRPVLLVGLVQLGHDKTISEGVRRFQIFFDDRNTSLPPDTRKAAYLSVMHNVSSTNRSGYDALLKIYRESTEVEERLNVLGILSSCQDKDIVLESLNFIFTDEVRNQDAY",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of Release of an N-terminal amino acid, Xaa-|-Yaa- from a   peptide, amide or arylamide. Xaa is preferably Ala, but may be most   amino acids including Pro (slow action). When a terminal hydrophobic   residue is followed by a prolyl residue, the two may be released as   an intact Xaa-Pro dipeptide..2. The protein requires Zn(2+) to activate a critical catalytic site.3. The designed protein should be involved in proteolysis, peptide catabolic process by localizing to membrane, endoplasmic reticulum, cytoplasm and exhibiting peptide binding, zinc ion binding, metalloaminopeptidase activity.4. The substrate, Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "DVASASNFRAFELLHLHLDLRAEFGPPGPGAGSRGLSGTAVLDLRCLEPEGAAELRLDSHPCLEVTAAALRRERPGSEEPPAEPVSFYTQPFSHYGQALCVSFPQPCRAAERLQVLLTYRVGEGPGVCWLAPEQTAGKKKPFVYTQGQAVLNRAFFPCFDTPAVKYKYSALIEVPDGFTAVMSASTWEKRGPNKFFFQMCQPIPSYLIALAIGDLVSAEVGPRSRVWAEPCLIDAAKEEYNGVIEEFLATGEKLFGPYVWGRYDLLFMPPSFPFGGMENPCLTFVTPCLLAGDRSLADVIIHEISHSWFGNLVTNANWGEFWLNEGFTMYAQRRISTILFGAAYTCLEAATGRALLRQHMDITGEENPLNKLRVKIEPGVDPDDTYNETPYEKGFCFVSYLAHLVGDQDQFDSFLKAYVHEFKFRSILADDFLDFYLEYFPELKKKRVDIIPGFEFDRWLKPNMAPTAPFHYQEQTDPVEYVENMCENMQLYPLQDILTGDQLLFEYKPEVIGEALNQLVPQKANLVLLSGANEGKCDLKEKWFGTQYSIEDIENSWAELWNSNFELNPDLHLPAENKYIATDFTLKAFDCPETEYPVKIVNTPQGCLWYKKDNKFKIPKAYIRFHLISPLIQKSAANVVLFDIFVNILTHNLAEPAYEADVAQLEYKLVAGEHGLIIRVKGFNA",
    "response#2": "DVASASNFRAFELLHLHLDLRAEFGPPGPGAGSRGLSGTAVLDLRCLEPEGAAELRLDSHPCLEVTAAALRRERPGSEEPPAEPVSFYTQPFSHYGQALCVSFPQPCRAAERLQVLLTYRVGEGPGVCWLAPEQTAGKKKPFVYTQGQAVLNRAFFPCFDTPAVKYKYSALIEVPDGFTAVMSASTWEKRGPNKFFFQMCQPIPSYLIALAIGDLVSAEVGPRSRVWAEPCLIDAAKEEYNGVIEEFLATGEKLFGPYVWGRYDLLFMPPSFPFGGMENPCLTFVTPCLLAGDRSLADVIIHEISHSWFGNLVTNANWGEFWLNEGFTMYAQRRISTILFGAAYTCLEAATGRALLRQHMDITGEENPLNKLRVKIEPGVDPDDTYNETPYEKGFCFVSYLAHLVGDQDQFDSFLKAYVHEFKFRSILADDFLDFYLEYFPELKKKRVDIIPGFEFDRWLGTPIVTVKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNSVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYPGAEGAGLLYQGEPGALNESISDIMGAMADRDDWEIGEDVYTPGIAGDSLRSLEDPSKQGNPDHYSNRYTGTEDYGGVHINSSIHNKAAYLLAEGGVHHGVQVEGIGREASEQIYYRALTYYVTASTDFSMMKQAAIEAANDLYGEGSKQSASVEKAYEAVGL",
    "response#3": "DVASASNFRAFELLHLHLDLRAEFGPPGPGAGSRGLSGTAVLDLRCLEPEGAAELRLDSHPCLEVTAAALRRERPGSEEPPAEPVSFYTQPFSHYGQALCVSFPQPCRAAERLQVLLTYRVGEGPGVCWLAPEQTAGKKKPFVYTQGQAVLNRAFFPCFDTPAVKYKYSALIEVPDGFTAVMSASTWEKRGPNKFFFQMCQPIPSYLIALAIGDLVSAEVGPRSRVWAEPCLIDAAKEEYNGVIEEFLATGEKLFGPYVWGRYDLLFMPPSFPFGGMENPCLTFVTPCLLAGDRSLADVIIHEISHSWFGNLVTNANWGEFWLNEGFTMYAQRRISTILFGAAYTCLEAATGRALLRQHMDITGEENPLNKLRVKIEPGVDPDDTYNETPYEKGFCFVSYLAHLVGDQDQFDSFLKAYVHEFKFRSILADDFLDFYLEYFPELKKKRVDIIPGFEFDRWLR"
  },
  {
    "reference": "MIELVFATNNAHKLEEVQAMVGDNIRLLTLADIGCTEDIPETGDTFEANASQKSAYLKERYGYDCFADDSGLEVEALNNEPGVYSARYSGTRDMEKNIDLLLSKLGNHPNRKARFRTVISLRLGREEFLFEGAVEGVIGYGRQGTGGFGYDPLFTPNGYDQTFAEMTAAEKNAISHRAIAVEKLTAFLKVRYQAG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must possess XTP diphosphatase activity to support nucleotide metabolic process under certain conditions.2. The Proton acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "GRHIVVPLALIVKGGKILMNKRNDPHVPQFHGVWEFQGGSVEYGETIEENVRREAKEESGYEVEVAEYLPTIEVKNRQYNDMRIQLYLVPIVCRIIRGDGKYNDQEVLEAKWYALDDVPNLNLFPGDGEMYRKFLY",
    "response#2": "GRHIVVPLALIVKGGKILMNKRNDPHVPQFHGVWEFQGGSVEYGETIEENVRREAKEESGYEVEVAEYLPTIEVKNRQYNDMRIQLYLVPIVCRIIRGDGKYNDQEVLEAKWYALDDVPNLNLFPGDGEMYRKFLY",
    "response#3": "GRHIVVPLALIVKGGKILMNKRNDPHVPQFHGVWEFQGGSVEYGETIEENVRREAKEESGYEVEVAEYLPTIEVKNRQYNDMRIQLYLVPIVCRIIRGDGKYNDQEVLEAKWYALDDVPNLNLFPGDGEMYRKFLY"
  },
  {
    "reference": "MESVSAGLRPRAGAGVPLAELAAHIGAIMAEGRGRVATIPDTPITGVTLRAQEVQPGDLFAALPGSATHGARHAAAAIERGAVSVLTDPAGVAELAKAAPDAPTRTVPVLVHPAPRSVLGDAAAAVYGHPSNRLTVVGITGTSGKTTTTYLVEAGLRAGGRVAGLIGTIGIRLDGTDLPSALTTPEAPALQALLAAMAERGVDTVVMEVSSHALTLGRVDGTRFAVGGFTNLSRDHLDFHPTMADYFEAKALLFDPASALRARTAVVCVDDEAGRAMAARAGDAITVSACAQTAKWLAVDVVPMGTGGQEFTAIDPAGMQHRVRIGLPGSYNIANCLLALAILDAVGVSPEQAAPGLREARVPGRLEQVDRGQDFLALVDYAHKPGALRAVLTALVHPDRRVAVVFGAGGERDPGKRAPMGTIAAELADLVVVTDDNPRGEDPAAIRRQILAGAAAAGGHAQVVEIGDRRDAIRHAVGWARRGDVVLVAGKGHETGQRGGGEVRPFDDRVELAAALEAFGRPEIRA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must possess magnesium ion binding to support regulation of cell shape, cell division under certain conditions. The designed protein sequence is ",
    "response#1": "VTGTNGKTSTVEMVRQLWRMAGFHAASIGTLGVTTADDRVSTGLTTPDIVTFLSNVAGLKREGVSHVAFEASSHGLSQYRTEGLAVRAAAFTNLSRDHLDYHGTMEAYLDAKLRLFSEVLEADGTAVLWADDPASARVAEVARARGIRVLSVGTTGETLKLVGRDPTLLGQGLVIEAEGKTYKVTLPLIGAYQAANALTSAGH",
    "response#2": "ITGTNGKTTTSFLIASLGESLGLKTAVMGTNGYGPLSSMKTQALTTPDPITLQKQLAELQQDGVELVAMEVSSHSLVQYRVNGCLFDTAVVTNISRDHLDYHGDIESYARAKALLVTWSGLKHAVINRDDDYAELFLNYSQAKKNWIYGYCATEKELPLPPQSILCKIRLQEQKQGKLQSLELDLGQHYSFATALIGRFNYQNLCAALN",
    "response#3": "VTGTNGKTSTVEMVRQLWRMAGFHAASIGTLGVTTADDRVSTGLTTPDIVTFLSNVAGLKREGVSHVAFEASSHGLSQYRTEGLAVRAAAFTNLSRDHLDYHGTMEAYLDAKLRLFSEVLEADGTAVLWADDPASARVAEVARARGIRVLSVGTTGETLKLVGRDPTLLGQGLVIEAEGKTYKVTLPLIGAYQAANALTSAGUG"
  },
  {
    "reference": "MDEQGHTTGKKVVVGVTGGIAAYKAAELVRLLVKSGLKTHVAMTEHATKFVSPLTFEALSGNRVVWDMWSHETSPMDHIHMGQESDLIVIAPATANFVAKTANGIGDDFLSTMVLAATARILVCPAMNTRMYMNSAVQRNLRQLKEAGMVVMDPSAGELACRTEGPGRLPEPEEIMEKISMLLSPQDLAGLKIMITAGPTREPIDPVRYLTNRSSGKMGFALAGAAAMRGAAVVLVSGPTALTPPCGVTFVPVTTTEEMRREVLGRSADCDAVIKAAAVLDYRPKNPADRKLKKENRSISIELEPTPDILAELGSGKQGRSILVGFAAETDDLLSNAKGKLERKNLDMIVANDVSRNDAGFDAETNLVKVLYRDGSVEDLPLMSKREVADCLLDRVKKMATEKGRR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should contain one or more DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains that are essential for its function.2. The protein should be able to bind CTP ligand in a variety of conditions3. The protein design must be able to perform metal ion binding, phosphopantothenate--cysteine ligase activity in order to contribute to coenzyme A biosynthetic process.4. The protein requires Mg(2+) to activate a critical catalytic site.5. The protein with the reaction H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2, the Proton donor must be optimized to ensure efficient catalysis.6. The protein design should consider the endogenous proteins and enzymes present in the CoA from (R)- pantothenate: step 3/5 pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MKIALGVTGGIAAYKAAEVLRLLQDRGVTVQVIMTRAAQEFVRPLTFAALSGQKVITEMFGEGNAEPNVDSAVEHISVAQGIDALLVVPATADSLAKFAQGIANDFLSTLYLATAAPVIVAPAMNVNMWEHPATKINLETLRARGVHVIEPGAGYLACGMVGAGRLAEPEHIATETDALAAAICRHFQPGSLQGKSVMITAGGTIERIDPVRYITNDSSGKMGFAIAEAARDYGAKVTLIAGQTQVEPPEGVEVIRVESTQDMYDAVMSRFDNMDIIVKAAAVSDYRPAVRADQKIKKTTDRMTIELVKNPDILQELGARKNGQFLIGFAAETHDVAAFAQDKLRRKQCDLLVANDVSRDDAGFGSNNNEVQVFFADGKEKRLPLMSKEEAADLLLDWVLTRKNP",
    "response#2": "MKIALGVTGGIAAYKAAEVLRLLQDRGVTVQVIMTRAAQEFVRPLTFAALSGQKVITEMFGEGNAEPNVDSAVEHISVAQGIDALLVVPATADSLAKFAQGIANDFLSTLYLATAAPVIVAPAMNVNMWEHPATKINLETLRARGVHVIEPGAGYLACGMVGAGRLAEPEHIATETEAVAEAAGRILSRTGDLAGRRVLVTAGPTHEPIDPVRYLANRSSGKQGFALAGALARVGAEVVLVAGPCALATPAGVTRVDVETAREMLSACRDALPVDAAICVAAVADWRVAETGAQKLKKDATGGAPPALALTENPDILATLAQLETGRPALVVGFAAETDRVLDHARAKRARKRCDWIVANDVSAATGIMGGDDTAVTVEMLGADGTRSLAGRHVVVSAGGTREYLDPVRYLGNRSSGRQGYALARTAALRGASVTLVAANVSLPDPAGVKVTRVGSTRELRDAVLAAARDADAVVMAAAPADFRPAGYADAKIKKADDGSAPTVELTQNPDILAELSHDRPRPGLVVAGFAAETGDATAGVLDHGRAKLARKGCDLLVVNEVGVDKGFESPDNEAVILFADGERVEVPRASKARLAEVVWDRVLPRLRAV",
    "response#3": "MKIALGVTGGIAAYKAAEVLRLLQDRGVTVQVIMTRAAQEFVRPLTFAALSGQKVITEMFGEGNAEPNVDSAVEHISVAQGIDALLVVPATADSLAKFAQGIANDFLSTLYLATAAPVIVAPAMNVNMWEHPATKINLETLRARGVHVIEPGAGYLACGMVGAGRLAEPEHIATETELLALVRRFFLAGRHVVVSAGGTREYLDPVRYLGNRSSGRQGYALARTAALRGASVTLVAANVSLPDPAGVKVTRVGSTRELRDAVLAAARDADAVVMAAAPADFRPAGYADAKIKKADDGSAPTVELTQNPDILAELSHDRPRPGLVVAGFAAETGDATAGVLDHGRAKLARKGCDLLVVNEVGVDKGFESPDNEAVILGADGSRRTLPRADKLTLAHRLWDTALELRR"
  },
  {
    "reference": "MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDPASQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPNHINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKHAVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQYIKDGAIVIDVGMNRDDNGKLIGDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQSALRSTHK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. We are looking for a protein with broad substrate specificity for 5,10-methenyltetrahydrofolate + H2O = (6S)-10-   formyltetrahydrofolate + H(+).2. Ideal protein should have a binding affinity for key enzymes in the One-carbon metabolism, tetrahydrofolate interconversion pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MSGKPLHEKTKEWARRHVRLLEEVGVTPKLAVLLLNDDPVELETQRRYVSLKARDVREVGGEVEIYELVDVPPERRSKEALYLIERLNRRDDVTGILIQKPVPPFVDEDLLFQRLSPEKDVL",
    "response#2": "MSGKPLHEKTKEWARRHVRLLEEVGVTPKLAVLLLNDDPVELETQRRYVSLKARDVREVGGEVEIYELVDVPPERRSKEALYLIERLNRRDDVTGILIQKPVPPFVDEDLLFQRLSPEKDVE",
    "response#3": "MSGKPLHEKTKEWARRHVRLLEEVGVTPKLAVLLLNDDPVELETQRRYVSLKARDVREVGGEVEIYELVDVPPERRSKEALYLIERLNRRDDVTGILIQKPVPPFVDEDLLFQRLSPEKDVL"
  },
  {
    "reference": "MFQDNPLLAQLKQQIRENIPKKEGVIRASDRGFGFLEVDEKTSYFVPPPYMKKVMHGDRVSALIRTEKEKEVAEPDALLEQSVTRFVARVKMFRDRLNVVADHPLIKDAIKARVKKGLDEKEFKEGDWVVATLKRHALVDGNFSAEITQTIASQEDHNVPWWVVLARHNLAQVEPADLPEWKVIEEEELPRTDLTATPFFTIDGAKTKDMDDALAIRKLDNGWELLVAIADPTAYVAEGSELDKEAAQRAFTVYMPGRNVPMIPRTLSDELCSLKEGEERNTLCARLLIAEDGLLLEETEFFAARISSHARLTYDDVSDWAEHGKQLDIDAGVLAQLPLMKAMTEARIAWRTEHALVFPDRPDYDFELGENGEVLAIHVEPRRIANRMVEESMIAANICAGRVLGKQVGYGIFNVHTGFDEESLDGAIDLLKSAEAPFEKEEIASLSGFCALRRWIDNLDTRWLDGKIRRFQSYALMSAEPGAHYGLGLDAYATWTSPIRKYGDMVNHRLLKAVIAGKTPGERPSLELTEHLTACRRLHRMVERDIGDWLYVRYLKADAGTDKVFNAEIIDVMRAGLKLRLRENGAVVFMPARHILDNKDRLECNWDNGRVYLDKTEVVYELGQIIEVKLTEAVEETRSLIAKPAVELVPGPAPVAPTSEADATPADEAPKAE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be optimized for exoribonuclease II activity. The designed protein sequence is ",
    "response#1": "LFDSDKKKPIPKHPKKIAVVTSPTGAVIRDIITISRRRNPTVDILVVPVLVQGSSAADEICNAFRILNKREDIDVIILARGGGSLEEIWPFNEEKVARCIYASRIPVVSAVGHETDFTISDFVADLRAPTPSAAAEIVVPDIKVYQRELFLLKTKLLTLMTAELNRKKKEFEGLKRALYLNSPTF",
    "response#2": "LFDSDKKKPIPKHPKKIAVVTSPTGAVIRDIITISRRRNPTVDILVVPVLVQGSSAADEICNAFRILNKREDIDVIILARGGGSLEEIWPFNEEKVARCIYASRIPVVSAVGHETDFTISDFVADLRAPTPSAAAEIVVPDIKVYQRELFLLKTKLLTLMTAELNRKKKEFEGLKRALYLNSPTS",
    "response#3": "LFDSDKKKPIPKHPKKIAVVTSPTGAVIRDIITISRRRNPTVDILVVPVLVQGSSAADEICNAFRILNKREDIDVIILARGGGSLEEIWPFNEEKVARCIYASRIPVVSAVGHETDFTISDFVADLRAPTPSAAAEIVVPDIKVYQRELFLLKTKLLTLMTAELNRKKKEFEGLKRALYLNSPTN"
  },
  {
    "reference": "MVLKPSSQQPVVIEELNRSELLYQELVCRMSSHKNKENQSGDPLLAKQKEHHRRAFEFISKALKIDEENEGHKEIAIKLYKRGIEELEKGIAIDCSHGKGDVWDRAQRLQEKMKTNLIMANDRLHLLTSEKKMLIGSVCRGNVLVKSKTLPRSMGSKSHNVNQVPAKITHTPPAIKRQFSGPCTSPVHKPTTKLKASVVKAGYLKGVDPKLAQIVMDEILEGGTPVHWDDVAGQEIAKQALNEMVILPSLRPELFTGLRTPARGLLLFGPPGNGKTLLARAVATASNSTFFSISAASLTSKYVGEGEKLVRALFSVARQLEPSIIFVDEVDSVLSERKEGEHEASRRLKTEFLVELDGLPASSNDSRVLVMAATNRPHELDEALLRRFPKRVYVCLPDMRTRAVLLQRLLSRQNNPLTLPEIETVAHLTEGYSGSDITALAKDSALAPIRELNPDQVRNLDPGKVRQITFQDFIQSLNRIRRSVSPSNLALYEKWNQQYGDVSM",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must have at least one MIT domain.2. The designed protein should have microtubule binding, ATP binding within membrane to regulate microtubule severing, protein hexamerization.3. The protein must exhibit the following characteristics: ATP-dependent microtubule severing protein. The designed protein sequence is ",
    "response#1": "PARGLLLFGPPGNGKTMLAKAVAAESNATFINISAASLTSKYVGEGEKTVRALFSVARELQPSIIFIDEIDSLLCERKEGEHDSTRRLKTEFLVQFDGVQSAGDDRVLVMGATNRPQELDDAALRRFAKRIYVSLPDLTRLARLTEGYSGSDITALAKDAALGPIRELRPEQLDMQAVADKLEGYTGSDISNVCRDAALMSMRRKVEGMTPEEV",
    "response#2": "VLMVGPPGTGKTMLAKAVATECKTTFFNVCSSSLTSKYHGESEKLVRLLFEMAHFYAPSTVFIDEIDSVCSKRGTSNEHEASRRVKSEILVQMEGVSTTQNADSDPSKMVIVLGATNFPWDIDEALRRRLEKRIYIPL",
    "response#3": "PWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARSYSPSTIFIDEIDSLCSQRGSDSEHEASRRFKAELLIQMDGLNSETNEDGKTIMVLAATNHPWDIDEAFRRRFEKRIYIPLPSREELIRR"
  },
  {
    "reference": "MYHHEDDTNSDMNSDDDMSRSGRETPPPRPSHAFGSERDLERRGRSRDVEPRDRWPYTRNPRSRLPQRDLSLPVMSRPHFGLDRDDDRRSMDYESRSQDAESYQNVVELKEDKKPQNPIQDNLENYRKLLSLGVQLAEDDRHSHMTQGHSSRSKRTAYPSTSRGLKPMPEAKKPSHRRGICEDESSHGVIMEKFIKDVARNPKSGRARELNERPPPRFPRPNDNWKDSSSSRRESVIQERGYEGSAFRGGFRFNADLASRSRALERKRRYHFDSDERGSGHEHKSCVRKKPFECGAEMRQAMSMGNLNSPSFSESQSIDFGANPYVCDECGRQFSVISEFVEHQIMHTRENLYEYGESFIHSVAVNEVQKGQGGGKRFECKECGETFSRSAALAEHRQIHAREYLAECRDQEDEETIMPSPTFSELQKMYGKDKFYECKVCKETFLHSSALIEHQKIHGRGNSDDRDNERERERDRLRARAREQRERERERERERELGEPFLTCPNFNEFRKMYRKDKIYECKVCGESFLHLSSLREHQKIHTRGNPFENKSRMCEETFVPSQSLRRRQKTYREKLFDFNNARDALMGNSDSSEHQKNRSRRNFFEGRGFEKPFVESQKSHTITRPPENKDDDKPFTISVNPNDKLKFPIMENGSQGKSYERSVIHSLGSAEAQKSHGGLGFSKPRPVAESSTQSSSSIYYPRAHSGGNTYEGKEYKDSIIHSLPAPRPLKRHRANDHIQCDEGGESSIYIPDIINKGRKIPAREDAY",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein's functionality will benefit from Basic and acidic residues, Polar residues compositional bias in its structure.2. The protein should have a specific function in regulation of transcription by RNA polymerase II and should be localized to nucleus in order to carry out this function efficiently.3. The protein must exhibit the following characteristics: Induces apoptosis in cooperation with SIAH1A; Acts synergistically with TRAF2 and inhibits TNF induced apoptosis through activation of NF-kappa-B. The designed protein sequence is ",
    "response#1": "EPTFYEDALSGGYAPPESGGYGYNNAKVLKQSMTLNLADPGSNLKPHLRNKSAELLTSPDVGLLKLASPELERLIIQSSNGLITTTPTPTQFLCPKNVTDEQEGFAEGFVRALAELHNQNTLPSVTSAAQPVSGGMAPVSSMAGGGGSFNAGLHSEPPVYANLSNFNPNALSSAPGYNANGMGYAPQHHLNAQMPVQHPRLQALKEEPQTVPEMPGETC",
    "response#2": "EPTFYEDALSGGYAPPESGGYGYNNAKVLKQSMTLNLADPGSNLKPHLRNKSAELLTSPDVGLLKLASPELERLIIQSSNGLITTTPTPTQFLCPKNVTDEQEGFAEGFVRALAELHNQNTLPSVTSAAQPVSGGMAPVSSMAGGGGSFNAGLHSEPPVYANLSNFNPNALSSAPGYNANGMGYAPQHHLNAQMPVQHPRLQALKEEPQTVPEMPGETC",
    "response#3": "EPTFYEDALSGGYAPPESGGYGYNNAKVLKQSMTLNLADPGSNLKPHLRNKSAELLTSPDVGLLKLASPELERLIIQSSNGLITTTPTPTQFLCPKNVTDEQEGFAEGFVRALAELHNQNTLPSVTSAAQPVSGGMAPVSSMAGGGGSFNAGLHSEPPVYANLSNFNPNALSSAPGYNANGMGYAPQHHLNAQMPVQHPRLQALKEEPQTVPEMPGETC"
  },
  {
    "reference": "MAESPTEEAATATAGAGAAGPGASGVAGVVGVSGSGGGFGPPFLPDVWAAAAAAGGAGGPGSGLAPLPGLPPSAAAHGAALLSHWDPTLSSDWDGERTAPQCLLRIKRDIMSIYKEPPPGMFVVPDTVDMTKIHALITGPFDTPYEGGFFLFVFRCPPDYPIHPPRVKLMTTGNNTVRFNPNFYRNGKVCLSILGTWTGPAWSPAQSISSVLISIQSLMTENPYHNEPGFEQERHPGDSKNYNECIRHETIRVAVCDMMEGKCPCPEPLRGVMEKSFLEYYDFYEVACKDRLHLQGQTMQDPFGEKRGHFDYQSLLMRLGLIRQKVLERLHNENAEMDSDSSSSGTETDLHGSLRV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Catalyzes the covalent attachment of ubiquitin to other proteins; Specific substrate for UBA6, not charged with ubiquitin by UBE1.2. The Glycyl thioester intermediate active site of the designed protein must be optimized for substrate binding and specificity.3. A protein that contributes to positive regulation of apoptotic process, ubiquitin-dependent protein catabolic process, protein ubiquitination, negative regulation of apoptotic process, apoptotic process by functioning in nucleoplasm with molecular adaptor activity, ubiquitin conjugating enzyme activity, cysteine-type endopeptidase inhibitor activity.4. The designed protein should interact with enzymes and substrates within the Protein modification pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "LGPFFSYGLESSARRPNHRVFVDCEEDARKYDGSVNTEQKLYFQRMDPIRTMLHQLMLPLASDQGSRNKTLRWIATIISTNDIRTRSHYDPSDVLCDHYMTNFLSVMYMFSEKIDLSKIIVDYPFLPSSLINISKETRLKMDESGAVAFASQFADRPDEYHFSTVCFFLTIAAQRLVIPPLMNQISEYSRHLKELKHKINALKEKLNTVSGFERAEVEKKLNYETEHWKLMSRHLLCVKTQAQDPALMASSMDFVDKQMKFILNLLCDNLDLLGDDSQLPTEVSQMFCALPEYFLEDALDFYIFAISNGMKLLMERNADWISRLTVLFTQYHYIKSPFLVSKLVRVLSSIQPPLWFNVVRLRMAQENLLMCMIKFYSDFEDNGDFYEKFNVRGNIQYMLEKMEEDMFYKGKFMDMARECGAEFIRFVNMVINDATWCIDESLSGLKSIHDVEKKMANKVEWDNTDQEIRNQDLGVYEEAKRKVKGL",
    "response#2": "LGPFFSYGLESSARRPNHRVFVDCEEDARKYDGSVNTEQKLYFQRMDPIRTMLHQLMLPLASDQGSRNKTLRWIATIISTNDIRTRSHYDPSDVLCDHYMTNFLSVMYMFSEKIDLSKIIVDYPFLPSSLINISKETRLKMDESGAVAFASQFADRPDEYHFSTVCFFLTIAAQRLVIPPLMNQISEYSRHLKELKHKINALKEKLNTVSGFERAEVEKKLNYETEHWKLMSRHLLCVKTQAQDPALMASSMDFVDKQMKFILNLLCDNLDLLGDDSQLPTEVSQMFCALPEYFLEDALDFYIFAISNGMKLLMERNADWISRLTVLFTQYHYIKSPFLVSKLVRVLSSIQPPLWFNVVRLRMAQENLLMCMIKFYSDFEDNGDFYEKFNVRGNIQYMLEKMEEDMFYKGKFMDMARECGAEFIRFVNMVINDATWCIDESLSGLKSIHDVEKKMANKVEWDNTDQEIRNQDLGVYEEAKRKVKGVHHFKLLNKEQYERYQRFATEEFVLKNGGVLCPQPGCGMGLLVDPECRRIQCQNGCGYVFCRSCLQGYHIGECFEC",
    "response#3": "DVDYCLKMLKFTRRLLLNTENRFVYSSGDVLMYLLNCPNFTIKLAVMRILAILGERFVIAREKIVAHNIFGDHNLRKKTLKLALSLSSSVMDEDGEHFSLVDLYFDKKKVPQKWRKLRFTHYTSNDFKKSSQQKNNINETQTSIKKVTMTTQELCEHSLQQIFDKGMALLPAESWFDFSIKASVAKAFSDDSGENIDLRNIIIETKLNAIAFVNTIFSPPQVSSKLFELDPYAFNSLTDLISLSETKIPKELRTDALFTLECISLKHVWCSDIIRNLGGNISHGLLFQILRYIR"
  },
  {
    "reference": "MRDRGKLVIWPVYLDQTKSRSSGRIISRKNAIKEPQLNEIKEAARQLGLNPEVEPHKAYPKSWWEVSGRVLVDDNGPKTVIAKQIALAIKKMRGPESSAKA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be involved in SRP-dependent cotranslational protein targeting to membrane by localizing to signal recognition particle and exhibiting 7S RNA binding.2. The protein must exhibit the following characteristics: Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane; Binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP.. The designed protein sequence is ",
    "response#1": "PTILLTVGIQGSGKTTTIAKLARHLQKRGYKVGLVCTDTWRPGAYYQLKQLVEPYNIEVFGDPGEKDAIKLAREGVEYFKDKGVDVIIVDTAGRHKEESGLIEEMKQISEAIKPHEVILVIDGTIGQQAYHQALAFKEATPIGSIIVTKLDGSAKGGGALSAVAATGAPIKFIGVGEKIDDLEPFDPKRFVSRLLR",
    "response#2": "YVVLLLGVEGSGKTTTAAKLAKYLAKRGYKVGLVETDTIRPAAFDQLKQLAEKIGVPFYGERDGKNAVEIAVRGVQNFKNMDVVIIDTAGRHRNEEELLKEVKAIYDAVKPDEVFLVIDATVGKLAAAQAEAFMKYLPIHSVIITKMDSTARGGGALAAVAKTGARVKFIGVGEDVDEFELFNPRKFVARVL",
    "response#3": "MLGNLGRSLSKTMKKLAGMTIVDEEVVREVIKDIQRALIQSDVNIKLVFNLSKSIEERALNEEPPKGITPKEHIISIVYDEMVKLI"
  },
  {
    "reference": "MKIAGVILAAGQGTRMKSTLPKVLHPVMGKPMIHYSIDTVTRVTGKKPYTVVGFDEQMIRQQLGDKIEYIIQDPQLGTGHALQTAAEVLQGKSDYILVTLADMPLIRQETMQAVVDAHVSGGGPITMLTVRNRDARGFGRVLRNTQGEVLSIVEEAQATQEQLQIEELNTSVYCFTASWLWQALDQIPMSPKGEYYLTDLMGIAVAGGKLVQTVALDDPVEAIGVNSRDHLAEVTYWMKKRINMDWMLSGVTIIDPDSTYIEGEVEIGTDSVIWPNTYLTGKSVIGKGCQVGPNAVVRNTQLGNRSKVVMSVLEEADVDEDVDIGPFAHLRKGARIGKGVHIGNFGEIKNSVLGEGTKMGHFSYIGDATIGPEVNIGAGTITCNFDGEKKNHTEIEEGAFIGSDTMLVAPVKIGKGARTGAGSVVTRDVPADTLAVGMPARMIKKLNKSD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECC",
    "response#2": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECH",
    "response#3": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECR"
  },
  {
    "reference": "MQGRVKWFNAEKGFGFIEREDGDDVFVHFSAIQQDGYKSLEEGQQVEFDIVDGARGPQAANVVKL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that contributes to negative regulation of termination of DNA-templated transcription by functioning in cytosol with RNA binding, transcription antitermination factor activity, RNA binding, nucleic acid binding, DNA binding. The designed protein sequence is ",
    "response#1": "GKILTGIVKKINRESITLDLGNNAEALILKEDMLPRENFRLGDRVRGVLYAIYPESRGAQLFVTRR",
    "response#2": "GKILTGIVKKINRESITLDLGNNAEALILKEDMLPRENFRLGDRVRGVLYAIYPESRGAQLFVTRS",
    "response#3": "GKILTGIVKKINRESITLDLGNNAEALILKEDMLPRENFRLGDRVRGVLYAIYPESRGAQLFVTRR"
  },
  {
    "reference": "MFVDVETAIADFRAGRFLIVTDDEDRENEGDLILPADSVTPEAINFMITHGRGLVCAPLTEERLAQLQLPPLPRRHSSGISTAFHMPVDVAGRTTTGTSAHDRAETVRALIDPATKPSDLIQPGHLFTLGARSGGVLQRAGHTEATIDLCRLAGLTLGGVLCEIVDDDGSMARLPRLVAFAERHGLHIVTIRALIEHRLKTEKLVRREVETVLPNRFATWRLVAYVNQVTHEHMEALVLGDVASGEPTLVRVHSQCFTGDTLGSLRCDCRSQLETAMARIASEGRGVLLYLHQEGRGIGLINKLKAYALQDQGQDTVDANVALGFKPDLREYGIGAQVLADLGLRRLRIMTNNPKKIVGLEAYDLEIVEQVPLEGGNTPENERYLETKRRRMGHRLNQTAKE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein with GTP binding, manganese ion binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, magnesium ion binding, zinc ion binding functions is required to enable riboflavin biosynthetic process.2. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.3. The protein design should consider the endogenous proteins and enzymes present in the riboflavin biosynthesis, Cofactor biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway to avoid interference with native metabolic processes.4. The D-ribulose 5-phosphate, Zn(2+), GTP binding site should be located in a region of the protein that is accessible to the ligand.5. The designed protein have GTP cyclohydrolase II domains with specific sequence motifs that are crucial for its activity.6. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.. The designed protein sequence is ",
    "response#1": "MKEKVKLPTEYGMFHLIPFRQANGLENMALIKGTWKEDEPVLVRVHSSCATGDILGSQRCDCGEQLHKAMEMIEKEGKGVIIYMQQEGRGIGLMNKIAAYKLQDQGLDTVEANLHLGFRPDERDYGCGAQMLRHLGIHKMRLITNNPTKRVGLEAYGLEIEENVPCTVVDGRRVATAHLPTQWGHFIAHAYEADIEANPYIALVMGDVTSEEPVLVRIHSGCLTGDALRSLRCDCGDQLDLAMRRIAEEGRGVLLYIQQEGRGIGLVNKLKAYVLQDHGADTVEANELLGFPADIRDYSLGAQVLEDLGLRRIRLMTNNPAKFAALEGYDLEIVERVERVAEAALPTAYGEFRAITYRTLIDAKEHQAFVMGNVDTDDPVLARVHDQCVTGDVFGSLRCDCGEQLEEAIRRVASEHRGVIVYMDQEGRGIGLHNKIRAYRLQDSGMDTVEANEALGLPADGREYGIGMQILVDLGVRKLRLMTNNPTKRSGLEGFGLEIVERVP",
    "response#2": "RLAETVLPTAIAGEFKAIVYESEVDPNHHLALVKGEIDSEQPILVRVHSQCLTGDVFGSLRCDCGSQLHTAMKMIEKEGKGVLVYMRQEGRGIGFANKMKAYALQDAGHDTVEANLALGFKPDLRNYGIGAQILRDLGVRKMRLMTNNPKKIVGLEGYGLEVVERIPMIGMRDKDRVATAHLPTQWGHFIAHAYEADIEANPYIALVMGDVTSEEPVLVRIHSGCLTGDALRSLRCDCGDQLDLAMRRIAEEGRGVLLYIQQEGRGIGLVNKLKAYVLQDHGADTVEANELLGFPADIRDYSLGAQVLEDLGLRRIRLMTNNPAKFAALEGYDLEIVERVERVAEAALPTAYGEFRAITYRTLIDAKEHQAFVMGNVDTDDPVLARVHDQCVTGDVFGSLRCDCGEQLEEAIRRVASEHRGVIVYMDQEGRGIGLHNKIRAYRLQDSGMDTVEANEALGLPADGREYGIGMQILVDLGVRKLRLMTNNPTKRSGLEGFGLEIVERVPLIVGRD",
    "response#3": "RLAETVLPTAIAGEFKAIVYESEVDPNHHLALVKGEIDSEQPILVRVHSQCLTGDVFGSLRCDCGSQLHTAMKMIEKEGKGVLVYMRQEGRGIGFANKMKAYALQDAGHDTVEANLALGFKPDLRNYGIGAQILRDLGVRKMRLMTNNPKKIVGLEGYGLEVVERIRVATAHLPTQWGHFIAHAYEADIEANPYIALVMGDVTSEEPVLVRIHSGCLTGDALRSLRCDCGDQLDLAMRRIAEEGRGVLLYIQQEGRGIGLVNKLKAYVLQDHGADTVEANELLGFPADIRDYSLGAQVLEDLGLRRIRLMTNNPAKFAALEGYDLEIVERVLRSPHRPDLPERIATTRLPTMHGDFRAYGYRDLVGGGEHLALVKGELSGDDPVLARIHSECLTGDAFGSLRCDCGPQLQAALAAVAKAGRGVVVYLRGHEGRGIGLAHKLQAYELQDNGHDTVDANIELGLPADARDYGVGAQILADLGVRSLALMTNNPAKLTGIEGYGLDVAERRVATAHLPTQWGHFIAHAYEADIEANPYIALVMGDVTSEEPVLVRIHSGCLTGDALRSLRCDCGDQLDLAMRRIAEEGRGVLLYIQQEGRGIGLVNKLKAYVLQDHGADTVEANELLGFPADIRDYSLGAQVLEDLGLRRIRLMTNNPAKFAALEGYDLEIVERVLLPLHRPDLPDLPDLPDLPDLPDLPDLPDLPDLPDLPDLPDLPDLPDLPDLPDLPDLPDLPDLPDLPDLD"
  },
  {
    "reference": "MCPGWWLWPLVAVCAADVFRDEAERIMQDTPVIDGHNDLPWQLLKMFNNQLQDERANLSTLTGTHTNIPKLRAGFVGGQFWSAYVPCDTQNKDAVRRTLEQMDVIHRMCEMYPETFLCVTTSAGIRQAFQEGRVASLIGVEGGHSIDSSLGVLRALYHLGMRYLTLTHNCNTPWADNWLVDSGSDDAQSHGLSVFGQSVLREMNRLGVMIDLAHVSVATMKAALQLSAAPVIFSHSSAYSLCANRRNVPDDVLQLVNQTGSLVMVNFYNDYVSCTEDADLSQVADHLDYIRKVAGAGAVGFGGDFDGVTRLPTGLEDVSKYPDLIAELLRRNWTEAEVRGALADNLLRVFEAVEKVSNETLQAPEEEPIPLDQLGSSCRTQYGYSEASTLYHQPGALLAALIFVLRLP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that contributes to glutathione catabolic process, leukotriene D4 catabolic process, neutrophil chemotaxis by functioning in apical plasma membrane with zinc ion binding. The designed protein sequence is ",
    "response#1": "TLTNACIALSQRWITAKEEDLNTFNATDLKDLSTHQVNEFLAQVLQQAPLPLGHVKRMQEVYNFNAVNNSEIRFRWLRLCIQSKWEEAIPLALKMATEQGRMKFTRPLFKDLAAFDKSHDQAIRTYQAHKASMHPVTAMLVGKDC",
    "response#2": "GNSFVVWSILKRMQKRTVTALLVLNLALADLAVLLTAPFFLHFLARGTWSFREMGCRLCHYVCGISMYASVLLITIMSLDRSLAVARPFMSQKVRTKAFARWVLAGIWVVSFLLAIPVLVYRTVKWNNRTLICAPNYPNKEHKVFHLLFEAITGFLLPFLAVVASYSDIGRRLQARRFRRSRRTGRLVVLIILAFAAFWLPYHLVNLVEAGRTVAGWDKNSPAGQRLRLARYVLIALAFLSSSVNPVLYAFGNT",
    "response#3": "TLTNACIALSQRWITAKEEDLNTFNATDLKDLSTHQVNEFLAQVLQQAPLPLGHVKRMQEVYNFNAVNNSEIRFRWLRLCIQSKWEEAIPLALKMATEQGRMKFTRPLFKDLAAFDKSHDQAIRTYQAHKASMHPVTAMLVGKDC"
  },
  {
    "reference": "MTKKIIIASHNQGKLQEIRTLLDPLPLNVVGAGDLLLPEPEEPFTTFIENALHKARYVSRLTASPALADDSGLCVDHLMGAPGIMSARFAGLDKSDEKNNQKLLDLLHNVSHRSAHFICCIVLVRHESDPDPIISIGKWYGQIARNQSGSNGFGYDPLFYIPELKKTAAQLTQAEKNQFSHRAKALQGIIQALQKDSYFND",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein with nucleotide binding, metal ion binding, XTP diphosphatase activity, ITP diphosphatase activity, ribonucleoside triphosphate phosphatase activity is needed to enhance nucleotide metabolic process, purine nucleoside triphosphate catabolic process in a specific context.2. The protein requires Mg(2+) to activate a critical catalytic site.3. The protein should have a high affinity for Mg(2+).4. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.5. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MMTYIIVASRNHDKTREITEILDSLGLSFEFSSQNKFPDIKESGTSFLENALIKAADVCRKTGIPAIADDSGLCVDYLHGAPGIFSARYAGVHGDDESNIRKLLEELKGVPDERRTARFHCVIVYMRHADDPSPVIAEGQWEGRILDERHGEGGFGYDPLFYVEELNRTAAEISVEEKNKISHRGKALKHLINILKERW",
    "response#2": "MDKIVIASGNAGKVKELQALLAPFGVSVVPQSAFNVTEAEEPHVTFVENALAKARHASKATGLPALADDSGLAVDALNGAPGVYSARYAGEHGNDDANNMLLLDQLRDVPDDQRQAHFHCVLVLMRHAEDPEPLICEGSWEGRILTEPRGDGGFGYDPYFYLPEHGCSAAQLPADHKNQLSHRGQAMAKLLERLQQ",
    "response#3": "MTGIVIATGNSGKAREFRALLLPLGVPVVPQSAFPVPEADETGLTFIENALIKARHAAAITGLPAIADDSGLCVDALGGAPGIFSARYAGEPKSDAANNAKLLAELADVPRERRAARFVCVLVLMRHADDPAPLIAEGSWEGRIAEAARGEGGFGYDPLFWVPERQCSAAELTPEVKNRISHRGAALRRMAALLAAR"
  },
  {
    "reference": "MTRIHPTAIVEPGAQIDESVEIGPYAIVGPHVTIGARTTIGSHSVIEGHTTLGEDNRIGHYASVGGRPQDMKYKDEPTKLVIGNRNTIREFTTIHTGTVQDVGVTTLGDDNWIMAYVHIGHDCRVGNNVILSSNAQMAGHVEIGDYAIIGGMSGVHQFVRIGAHSMLGGASALVQDVPPFVIAAGNKAEPHGINVEGLRRRGFSPDAISALRSAYRLLYKNGLSLEEAKVQLRELAVAGGEGDAAVTAFVEFIDASQRGIIR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell..2. We are looking for a protein with broad substrate specificity for a (3R)-hydroxyacyl-[ACP] + UDP-N-acetyl-alpha-D-glucosamine =   a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine + holo-   [ACP].3. The protein must exhibit acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity.4. The designed protein should interact with enzymes and substrates within the lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 1/6, Glycolipid biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MTTLFISDLHLDPARPAITELFLEFLRTQVPGSDALYILGDLFEAWIGDDTPSTAADAVAVALHAVADSGVPVFFMAGNRDFLVGETYAQRAGFRILPDPTVIDLYGHTTLLMHGDLLCTDDTAYQAFRAQTRDPVFQAQFLAQPLAARVAFAQQARAASQARHAELKQGDQSRFETVTDVSPAEVEATFVRYGLDRLIHGHTHRPAVEDGPWSEDVAAFTMVSGNPAKAHGLNIEGMRRKGWSKDSIDVLRQAYRVVFRSGLTTVQALEVLKQDLLPKESKIEFLIDSLQKSRRGVDCIPYGMVLGNRAYLSGLNIIGLQRRGFAREDIHALRRAYRLLFAPEGTLMERVEDVAATFESHAAVAEILDFIRLGGKRSIDCIPYGMVLGNRAYLSGLNIIGLQRRGFAREDIHALRRAYRLLFAPEGTLMERVEDVAATFESHAAVAEILDFIRLGGKRSIKN",
    "response#2": "MTTLFISDLHLDPARPAITELFLEFLRTQVPGSDALYILGDLFEAWIGDDTPSTAADAVAVALHAVADSGVPVFFMAGNRDFLVGETYAQRAGFRILPDPTVIDLYGHTTLLMHGDLLCTDDTAYQAFRAQTRDPVFQAQFLAQPLAARVAFAQQARAASQARHAELKQGDQSRFETVTDVSPAEVEATFVRYGLDRLIHGHTHRPVHVNPRHPGSVWDEGVDVAGFTRIVDRIVNGDGPTG",
    "response#3": "MTTLFISDLHLDPARPAITELFLEFLRTQVPGSDALYILGDLFEAWIGDDTPSTAADAVAVALHAVADSGVPVFFMAGNRDFLVGETYAQRAGFRILPDPTVIDLYGHTTLLMHGDLLCTDDTAYQAFRAQTRDPVFQAQFLAQPLAARVAFAQQARAASQARHAELKQGDQSRFETVTDVSPAEVEATFVRYGLDRLIHGHTHRPDIHRPDPNRFDCIPYGMVLGNRAYLSGLNIIGLQRRGFAREDIHALRRAYRLLFAPEGTLMERVEDVAATFESHAAVAEILDFIRLGGKRSIDIPPFCLAEGNKAVLRGLNLTGLRRRFDNREDIDAIKHAYRELFEVGKPLQDVARELLDNDKNKYVKELASFVLNTKRGIDIPPFCLAEGNKAVLRGLNLTGLRRRFDNREDIDAIKHAYRELFEVGKPLQDVARELLDNDKNKYVKELASFVLNTKRGI"
  },
  {
    "reference": "MVRGAVPRTTEFGELLYGKAVNLGGRVLIYGLGRSGRGVAHFLHGEGVSAFWHDLRPAPEDEALMRQLGHRQADLGGTYDLVVAAPGVPIDHRDLRVLAGRGAEIIGEVALAARLRPELPMVGITGTAGKGSTTVLIAQLLRACGLRAREGGNIDPPLLDVVDDAEVAVVELSSFQLERVPGLRLPVAVITNLGVDHLDRHGSVETYHAAKLNITAGQQSGDVLVRPADLPVPTRAQTVTFTPERLCLRDGQEVLPVADLPPGVHPANAAAALLAAEALLRHLGRAVDPAVLADALRAAQPVKGRFETVGQLGEVGFIEDSIATRTIAVESALRQARPPIAWLVGGRDKGAELAPLRAAAEGRVTRVIAFGEDGEALARDLGLPFEVIKAETGDESMDRAVRAGWEALGGAGGTGTVLLAPVGTSFDQFRDYQQRGASFRRAVQALLGTQSGSGESA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that contributes to cell division, peptidoglycan biosynthetic process, cell wall organization by functioning in cytoplasm with ATP binding, UDP-N-acetylmuramoylalanine-D-glutamate ligase activity.2. We require a protein that can specifically bind to the metabolites produced in the Cell wall biogenesis, peptidoglycan biosynthesis pathway. The designed protein sequence is ",
    "response#1": "VTGTNGKTTVTTIINHLLTDNELSTALIGTNGFQINKDIYSSSNNTTSDVLTNQHMLYEAKNQGVTHVAMEVSSHGLEQGRLWGIDFTVATFTNLSHEHLDYHGSMEEYAATKVQLFSSLAIQPNKQKIAVLNRDDNWYEYFQKRTPVQIVSYGIYEQADFRATAIKYYKDRTSFELLTPEGSFQVQTKLMGEFNVYNVLAAIALVTGSNGKTTTVMLTQQIMDHYLQKQGHHAYAVGNIGWPISEVVLNQAGPDDLLVVEMSSFQLMGVTDIEPKVAAIVDIYNNVHLDYHKTFDNYVDAKLNVGRFQKTSDYFLANFDQKDILAREEKATKAKILTFSENDPVADFYIGQDYLMHGEEKMMKIADIKLPGIHNLQNSLVAIGGRMEKLESSGNRHIYIDYAHTPDALTKAINSLLPFKQNKLIVVVGTGGDRDKSIRPLLAKAASVADYVILTINDPRHEDPNNILQDLENGMLHHQYTCLENRKEAIREAINYSSPNDIILIAGKGAAE",
    "response#2": "VTGTNGKTTVTALTRQLIEACGLSARAAGNISPAALASLMEALDQDALPDVWVLELSSFQLETTHSLQADAAVVLNVTQDHLDWHGDMQAYAQAKARLLKMARVAIVNRDDPLTLAMVADVNGLNVRSFGRR",
    "response#3": "VTGTNGKTTVTALTRQLIEACGLSARAAGNISPAALASLMEALDQDALPDVWVLELSSFQLETTHSLQADAAVVLNVTQDHLDWHGDMQAYAQAKARLLKMARVAIVNRDDPLTLAMVADVNGLNVRSFGRE"
  },
  {
    "reference": "MVTIRLRRTGKTKKPSYRLVVADSRAPRDGRFIEIVGHYNPVRQPKELNVKADRVRYWLGVGAQPSETVVRLLKQVGVLDADGKPAPVTPIEG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a specific function within the ribonucleoprotein complex. The designed protein sequence is ",
    "response#1": "GPDFFTDVIVIETADHARLQLQLAYNWHFEVKDRSDPMESAKVFSVLDFVGDACKAIASRIRGAVASVQFDDFHKNSSRILRAAVFGLDPELRVRERLLFPQNSLCVTSVDVHSVEPVI",
    "response#2": "GPDFFTDVIVIETADHARLQLQLAYNWHFEVKDRSDPMESAKVFSVLDFVGDACKAIASRIRGAVASVQFDDFHKNSSRILRAAVFGLDPELRVRERLLFPQNSLCVTSVDVHSVEPVL",
    "response#3": "GPDFFTDVIVIETADHARLQLQLAYNWHFEVKDRSDPMESAKVFSVLDFVGDACKAIASRIRGAVASVQFDDFHKNSSRILRAAVFGLDPELRVRERLLFPQNSLCVTSVDVHSVEPVA"
  },
  {
    "reference": "MDENVAEFLRRIILKIPLCEMKTILEAWDFLSEDQLQSINLKQRKEFLAQEVILLCEDKCASLDDMALLDLMYTQFHRHQKLWNVFQMSKEPGGNDVDLFDMEQFQSSFKRILQRALKNVTVSFRVYEKNSVWIRVAWGTQYSQPNQYKPTFVVYYPQTPYVFISSCYLKSTVPLLHQALKVASKHHQMAQLDLRSRHLDSLTAIVFNEYDQTFENYNSTASWREGSLGLKTDLDSKIIHENTEEKVRIHRATQEAFGAYPQPQLEFAQYKLETKFKSDVGGGILADRKEPLRCLVKFSSPHLLEALKSLAPAGIADTPLSPLLTCIPSKKMNYFKIRDK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be designed to localize specifically to nucleoplasm in order to perform its role in kinetochore assembly more effectively.2. For general function, the protein need meet that CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly The designed protein sequence is ",
    "response#1": "PQTPRRRPSSPAPGPSRQSSSVGSQTLRRRQKFMWLKEIKTLQKSTDLLFRKKPFSMVVREICEKFSRGVDFWWQAQALLALQEAAEAFLIHLFEDAYLLSLHAGRVTLFPKDIQLTRRIR",
    "response#2": "EHLCARTHLKQLVMEFDTACPPDEGCNSGAEVNFIESAKETLEEVGKVKSAFESKALVIKRIQLMDALRKRVKENDGCARLIVETMRDIIKLNWEIIQAHQQARVIRENLNDIRRKRYFLKQAEGEKALRIFTTVRKKKEVVRMKIAEKLKFIHRNVQYERKVTTLVQNILQNIIVGCQINWAKDPSLRAIILQLF",
    "response#3": "LLLRLRAQTKQQLLEYKSMVDANEEKTPEQIMQEKQIEAKIEELENEVEEAKTAFEMKKLALDRMQLSTALKKHLEKVDIKTSVLMDNMKQILNLNKLIMKSQQETWDLEEKLLDVRKKRLQLKQASERKLLEIQTEKNKQKDDLDSMENSDKIKAIQQNLETEIQITTVIQHVFQNLILGSKVNWAEDPALKETVLQLLG"
  },
  {
    "reference": "MAAAIDGSLPVCRQSAALIDGVHTDFLASWYSDRILVLVTQFQKFGTLVSVTRDQPVARPDQAQGGTDSHTFTTKVLMGDDLPIWHVYGQQIFKAINGEDGCKPVLVAIALQNHSPEILKCILGQLESIRQVQTP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be able to regulate proteasome assembly.2. For general function, the protein need meet that May cooperate with psmg1-psmg2 heterodimers to orchestrate the correct assembly of proteasomes .; Chaperone protein which promotes assembly of the 20S proteasome The designed protein sequence is ",
    "response#1": "GQAA",
    "response#2": "LHHHHHH",
    "response#3": "GHHHHHHY"
  },
  {
    "reference": "MGSREAGLWVSFFLLQMATGVMSSQPCEKKYFGRDAMVCVCNRTYCDTHDPVSLPPAGSYVMYESSKSGSRLERSEGRFQNKTFTPDLLLVLETAQRYQVIKGFGGSITDSVAINVLSLTPDTQDNLLRSYFSDQGIEYNLLRMPMASCDFSVRLYTYDDWPYDFELQNFSLVDEDVKMKIPLIHRARAMSKKNISLCASPWTSPLWMRTNKEWKGKGSLLGQAGDRYHKTWANYFIRFLDEYAKHDVTFWAVTAENEPSAGLIDNYPFQSLGFTPEQQRDFIALDLGPALANSSHKAVRLIILDDNRIHLPNWAKVVLSADSQARRYVHGIATHWYLDFIAPIADTVAITHQLFPDYFLISTEACIGSHFWERDVILGSWDRGNQYSRNILENLNNHVVGWIDWNLALDLEGGPNWVQNYVDSPIIVDVANDMFYKQPMFYHMAHFSKFIPEGSQRVGLRVEKKCFGCSLEHMAALRPDGSAMVVVLNRSGSKAPFAISDTGRLIVTQIPANSIQTYLWRRE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design should consider the endogenous proteins and enzymes present in the Steroid metabolism, cholesterol metabolism pathway to avoid interference with native metabolic processes.2. We are looking for a protein with broad substrate specificity for cholesteryl 3-beta-D-glucoside + H2O = cholesterol + D-   glucose.3. Design the protein with a signal peptide incorporated.4. The protein can be expressed in lysosomal membrane and perform glucosylceramidase activity, glycosyltransferase activity without triggering undesirable cellular responses.5. The designed protein should contain one or more Glycosyl hydrolase family 30 TIM-barrel, Glycosyl hydrolase family 30 beta sandwich domains that are essential for its function. The designed protein sequence is ",
    "response#1": "VKGFGGSITDAAAINILSLPETAQDHLLRSYFSEEGLEYNLIRIPMASCDFSLHVYTYDDVPFDYELAHFSLRDEDTKMKIPLLHRALAMSKQPLSLYASPWTSPTWMKSSESFVGKGTLKGQAGDKYHKTWANYFVRFLDEYAKHNLTFWAVTAENEPTAGLINNYPFQCLGFTAEQQRDFIARDLGPALANSSHRHIRLIILDDNRIQLPHWAKVVLEDEEAARYVHGIGIHWYLDFIGPIQDTVVPTHELFPDYFILATEACIGAHFWERDVILGCWERGNQYSHSILTPMFYHAA",
    "response#2": "MPMASCDFSVRPYTYDDFPYDYELKNFSLVGEDLLMKIPIVHRAKAMSKRPISLMASPWTAPIWMKSNEAWKGKGWLKGEAGDSIHKTWASYFVRFLDEYAKQNLTFWAITAQNEPSSGFVKDYPFQTLGFTAESQREFIASDLGPALAKSSHKNIRLITLDDNRLHLPKWAKEVLSTESQAYRYIHGIGLHWYMDFISPIKGTVEATQRLFPDYFLIATEACAGSFFWEQDVILGSWDRGEDYSHSILENLNHHVVGWVDWNLALDLQGGPNWVKNYVDSPIIVDASSDIFYKQPMFYHLGHFSKFIPEGSQRVGLHSSRRCLTCQLQHVAALRPDGALVLVVLNK",
    "response#3": "MPMASCDFSVRPYTYDDFPYDYELKNFSLVGEDLLMKIPIVHRAKAMSKRPISLMASPWTAPIWMKSNEAWKGKGWLKGEAGDSIHKTWASYFVRFLDEYAKQNLTFWAITAQNEPSSGFVKDYPFQTLGFTAESQREFIASDLGPALAKSSHKNIRLITLDDNRLHLPKWAKEVLSTESQAYRYIHGIGLHWYMDFISPIKGTVEATQRLFPDYFLIATEACAGSFFWEQDVILGSWDRGEDYSHSILENLNHHVVGWVDWNLALDLQGGPNWVKNYVDSPIIVDASSDIFYKQPMFYHLGHFSKFIPEGSQRVGLHSSRRCLTCQLQHVAALRPDGALVLVVLNK"
  },
  {
    "reference": "MNEVSVVKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEVPDQTLPPLNNFSVGEEWMRAIQMVANSLKNQEPVEDSMDYKCGSPSDPSVAEEMEVAVSKTRTKATMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVIIAKDEVAHTVTESRVLQNTRHPFLTALKYAFQTNDRLCFVMEYANGGELFFHLSRERVFTEDRARFYGAEIVSALEYLHSRDVVYRDIKLENLMLDKDGHIKITDFGLCKEGITDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMTCGRLPFYNQDHERLFELILMEEIRFPRTLSPEAKSLLAGLLKKDPKQRLGGGPRDAKEVMEHRFFSAINWQDVLQKKLLPPFKPQVTSEIDTRYFDDEFTAQSITITPPDRYDSMGSLEPDQRTHFPQFSYSASIRE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that can perform ATP binding, protein serine/threonine kinase activity in cytosol, cell cortex, nucleoplasm, protein-containing complex, ruffle membrane to support insulin receptor signaling pathway, peripheral nervous system myelin maintenance, protein phosphorylation, positive regulation of cell migration, positive regulation of mitochondrial membrane potential.2. The designed protein must have at least one AGC-kinase C-terminal, PH, Protein kinase domain.3. We need a protein that can perform ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-   threonyl-[protein] with high efficiency.4. The protein should be able to bind ATP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "IGKGCSAAVYEATMPVLPQNQQVAGSIGLLPGRGPEIIPRGEEEQAPLAPAFPLAIKMMWNISAGSSSEAIFSTMSQELVPASRVALAGEYGAVAHRRSKGGPKQLAAHPNVIRVFRAFTSSVPLLPGALLDYPDVLPPRLHPEGLGHGRTLFLVMKNYSCTLRQYLRGNSPSPRLATVMTLQLLEGVAHLVQQGVAHRDLKSDNVLVEPDADGCPWLVITDFGCCLADERVGLQLPFTSWYVDRGGNGCLMAPEVSTACPGPRAVIDYSKADAWAVGALAYEIFGLPNPFYGQGSRAHLESRSYQEAQLPALPESVPPEARQLVRSLLQRDTSKRPSARVAANLLHLSLAAHPNVIRVFRAFTSSVPLLPGALLDYPDVLPPRLHPEGLGHGRTLFLVMKNYSCTLRQYLRGNSPSPRLATVMTLQLLEGVAHLVQQGVAHRDLKSDNVLVEPDADGCPWLVITDFGCCLADERVGLQLPFTSWYVDRGGNGCLMAPEVSTACPGPRAVIDYSKADAWAVGALAYEIFGLPNPFYGQGSRAHLESRSYQEAQLPALPESVPPEARQLVRSLLQRDTSKRPSARVL",
    "response#2": "IGKGCSAAVYEATMPVLPQNQQVAGSIGLLPGRGPEIIPRGEEEQAPLAPAFPLAIKMMWNISAGSSSEAIFSTMSQELVPASRVALAGEYGAVAHRRSKGGPKQLAAHPNVIRVFRAFTSSVPLLPGALLDYPDVLPPRLHPEGLGHGRTLFLVMKNYSCTLRQYLRGNSPSPRLATVMTLQLLEGVAHLVQQGVAHRDLKSDNVLVEPDADGCPWLVITDFGCCLADERVGLQLPFTSWYVDRGGNGCLMAPEVSTACPGPRAVIDYSKADAWAVGALAYEIFGLPNPFYGQGSRAHLESRSYQEAQLPALPESVPPEARQLVRSLLQRDTSKRPSARVAANLLHLSLSPSDNSGAEEMEVSLAKPRHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIAWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGP",
    "response#3": "VMGDVLGEGSYGKVKEAMNSENLTRLAVKILTKRKLRRIPNGEQNVTREIHLLKQLKHHNVVQLVDVMYNEEKQKMYLVMEYCIGGLQEMLDHAPEKKMPLYQAHGYFKQLVNGLEYLHSCRVIHKDIKPGNLLLTLDQTLKVSDFGVAEQLDMFAPDDTCTTGQGSPAFQPPEIANGHETFAGFKVDIWSSGVTLYNLVTGLYPFEGDNIYRLLENIGRSQWIAPDWLYELDESFAKLILGMLQADPSARLSLQQIRKDDWFELKKVLGKGGYGKVFQVRKTAGRDANKYFAMKVLKKASIVTNQKDTAHTRAERNILEAVKHPFIVELVYAFQTDGKLYLILEYLSGGELFMHLEREGIFLEDTTCFYLSEIILALGHLHKLGIIYRDLKPENILLDAQGHVKLTDFGLCKEHIQEGIVTHTFCGTIEYMAPEILTRSGHGKAVDWWSLGALMFDMLTGVPPFTAENRKKTIETILKAKLNLPAYLTPEARDLVRRLMKRQEPQRLGSGPEDAAAVQIHPFFAGIAWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSP"
  },
  {
    "reference": "MPQLRSHTTTQGRNMAGARALWRATGMTDDDFDKPIVAVSNSFTQFVPGHVHLKDLGQLVAGEIAAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIADSVEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTVFVSGGPMEAGKTKLAGVSVKLDLINPMISAGDASVSDEDLLEMERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGTTLATHADRRELFCEAGRRVVDLARRYYEKDDESVLPRSIATKTAFENAMRLDIAMGGSTNTVLHILAAAREGEVDFTMADIDRLSRSTPNICKVAPSTDRYHVEDVHRAGGIMGILGELDRGGLLETSVPTVHSNTLADALEVWDVMRDPSSEVVEFYRAGPAGIPTQQAFSQSERWPTLDTDRAGGCIRSVDHPYSTDGGLAVLYGNIAADGCIIKTAGVDESIFRFSGPARVFESQEEACDGILDEEQGIQAGDVVVVRYEGPRGGPGMQEMLYPTSYIKSRGLGKECALFTDGRFSGGTSGLSIGHASPEAAEGGAIALIRDGDMIDIDIPNRTVNLRVDDAEFTRRRQQEDARSDGWQPRDRQRQVSAALRAYAAMATSADKGAVRDLSRIEA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be designed to have high stability and activity towards (2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-   oxopentanoate + H2O.2. The protein should be optimized for activity in L-valine from pyruvate: step 3/4 pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. For general function, the protein need meet that Functions in the biosynthesis of branched-chain amino acids4. The stability of the protein-[2Fe-2S] cluster complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "VHPTSTPRSPTGGLVVLKGNLAPDGAVMKVAGTTYLKHEGPARVFDSERAAFNAVVSGEVEPGVSMVVRYEGPKGGPGMQEMLSVTSAIVGKGLGESVMLITDGRFSGATRGPMIGHVAPEAAVGGPIALIEDGDILAMDAGRRELNVMLSDNELTRRRALWAPPPPNYTTGVLAKYAKLVSSASEGC",
    "response#2": "VVRTFAKPVMARGSYAVIYGNVAPDGAVIKLVGHDTAHFEGPARVFDSEDAAFDAVQAGGISEGDVIVIRYEGPKGGPGMREMLQVTAALKGRGMQNVALITDGRFSGASYGFVAGHVSPEAATGGPIALLRDGDIIRIDVAARSINTSADLSARTAQPPTREAPVGAFAKYAVLVASASQGGCDKITPGMMMVK",
    "response#3": "DVVRPVAAPLKPRGGYSILYGDLAPEGCILKLAGHGREHFAGPARVFDSEEAAFAAVQARRINAGDVVVIRFEGPAGGPGMREMLAVTAALVGQGLGNDVALITDGRFSGATHGFMVGHIAPEAARGGPIAKLREGDTVRIDVAQRRIDVDANLATRPVARMAPRVTTGVLAKYARAVGSASRGG"
  },
  {
    "reference": "MAATLGRDQYVYMAKLAEQAERYEEMVQFMEQLVTGATPAEELTVEERNLLSVAYKNVIGSLRAAWRIVSSIEQKEESRKNDEHVSLVKDYRSKVESELSSVCSGILKLLDSHLIPSAGASESKVFYLKMKGDYHRYMAEFKSGDERKTAAEDTMLAYKAAQDIAAADMAPTHPIRLGLALNFSVFYYEILNSSDKACNMAKQAFEEAIAELDTLGEESYKDSTLIMQLLRDNLTLWTSDMQEQMDEA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be optimized for stability and expression in nucleus, cytosol, plasma membrane, cytoplasm, plant-type cell wall where it will participate in positive regulation of protein catabolic process, brassinosteroid mediated signaling pathway, response to cold, response to freezing.2. The protein must exhibit the following characteristics: Mediates F-actin dynamics possibly through inhibiting ADF1 phosphorylation; Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes ; Negative regulator of freezing tolerance that modulates cold-responsive C-repeat-binding factors (CBF) DREB1A and DREB1B proteins stability by facilitating their ubiquitin-mediated degradation when activated by CRPK1-mediated phosophorylation in freezing conditions.. The designed protein sequence is ",
    "response#1": "LFIEDALENLEREPEYSQEIGADLEVGRLQKDKRSFLRSKIEGTSGSYPFENLRTAADMLFLHGGSDLVVAKQAIFLYYLFDRHWTTPEKYWKHTIDDFAATFGITRHSLLESFVFYLLDDHSEEALQEACRILPEICGPETYPKVAQVLLERDNPETALMVLRWSGRDGVSELVSIGEAVTALRVRVECGLLSEAFTYQRTLCLKVKENNLKNGAVKHASDDLDIWSWTEWMEILVNEFCCLSIRRNLVDRIIELPWNPDEEKYLHRCLLNSGRC",
    "response#2": "LFIEDALENLEREPEYSQEIGADLEVGRLQKDKRSFLRSKIEGTSGSYPFENLRTAADMLFLHGGSDLVVAKQAIFLYYLFDRHWTTPEKYWKHTIDDFAATFGITRHSLLESFVFYLLDDHSEEALQEACRILPEICGPETYPKVAQVLLERDNPETALMVLRWSGRDGVSELVSIGEAVTALRVRVECGLLSEAFTYQRTLCLKVKENNLKNGAVKHASDDLDIWSWTEWMEILVNEFCCLSIRRNLVDRIIELPWNPDEEKYLHRCLLE",
    "response#3": "LFIEDALENLEREPEYSQEIGADLEVGRLQKDKRSFLRSKIEGTSGSYPFENLRTAADMLFLHGGSDLVVAKQAIFLYYLFDRHWTTPEKYWKHTIDDFAATFGITRHSLLESFVFYLLDDHSEEALQEACRILPEICGPETYPKVAQVLLERDNPETALMVLRWSGRDGVSELVSIGEAVTALRVRVECGLLSEAFTYQRTLCLKVKENNLKNGAVKHASDDLDIWSWTEWMEILVNEFCCLSIRRNLVDRIIELPWNPDEEKYLHRCLLG"
  },
  {
    "reference": "MALVVQKYGGSSVADAERIRRVAERIVATKKQGNDVVVVVSAMGDTTDDLLDLAQQVCPAPPPRELDMLLTAGERISNALVAMAIESLGAHARSFTGSQAGVITTGTHGNAKIIDVTPGRLQTALEEGRVVLVAGFQGVSQDTKDVTTLGRGGSDTTAVAMAAALGADVCEIYTDVDGIFSADPRIVRNARKLDTVTFEEMLEMAACGAKVLMLRCVEYARRHNIPVHVRSSYSDRPGTVVVGSIKDVPMEDPILTGVAHDRSEAKVTIVGLPDIPGYAAKVFRAVADADVNIDMVLQNVSKVEDGKTDITFTCSRDVGPAAVEKLDSLRNEIGFSQLLYDDHIGKVSLIGAGMRSHPGVTATFCEALAAVGVNIELISTSEIRISVLCRDTELDKAVVALHEAFGLGGDEEATVYAGTGR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The engineered protein is expected to adopt a strand-strand-helix-strand-turn-strand-turn-helix-helix-strand-strand-helix-helix-strand-strand-based conformation.2. The protein have enhanced ATP + L-aspartate = 4-phospho-L-aspartate + ADP.3. A protein that can perform ATP binding in cytosol to support threonine biosynthetic process.4. A protein that is capable of catalyzing reactions within the L-threonine from L-aspartate: step 1/5 pathway with high efficiency and specificity.5. The designed protein should have a high specificity for ATP, substrate binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "KVGLIGWRGMVGSVLLQRMRDERDFDLIEPLFFTTSNVGGKAPPESRGAPLKDAHDLNALKAMDTIISCQGGDYTTAIFPKLRAAGWQGYWIDAASTLRMDKNAVIILDPVNLGVIEEALAKGGTKNWIGGNVNCTCVRVPVMRSHLGPLHEKYRLKRVIISTYQSVTGTGKAAVDQLNAETEFSVNGYKDTESNISKVYPYQIFKNALPHCDVFSDDGYTKEEIKLMKEPKKILGDDSFSLTATAVRVPIQGGHSESVNIEFENEFDLDEVRKILSETPGVVVMDNVEKNEYPMPLYSEGKDDVFVGRMRRDLTQPKTMNLWIVADNLRKGAALNTLKPIQDHFGLSRVIVSTYQAVSGAGAQANNELEEQSKQYLNGEEMQAEILPVKGDKKHYPIAFNALPQIDVFQENGYTFEEMKMINETKKIMHTPELSVTATCVRLPFFTSHAESIYVEVEKDGLSVKDIWEVLENADGVLLEDDPSTQTYPTPLSAANKEEVFVGRVRKDLDHEKGFHMWVVSDNLIYGTAEATKELVDPLHKLAGLRRLIVSTYQAASGAGMPGLTELQREMKVVANGGEMGDTSPFAYQLAANLIPQIGSFKDEGYTSEEWKMQNEGRKIMHLPEVRVNCTCVRVPVMRSHSESITAEFDRPISPDEAREALAAAPGVRVVDDPANLRYPMPLDTSDQDLVYVGRIRRDLSAPDDVNSLTFWCCGDQIRIASTRMLGQVGFLAKVFSIFEELGISVDVVATSEVSISLTLDPSKLWSRELIQQELDHVVEELEKIAVVNLLKGRAII",
    "response#2": "LKPIYDAVGIERINVATYQAVSGSGKEAVDELARQTAELMNGKEVTVKTYPWQIAFNVLPQIDAFQDNGYTREEMKMVWETQKIMSDKTIKVNPTAVRVPVFYGHSEAVHIETSQPIASADARKLLSESSGITVLDNPENSEYAMPALQGAGKDTVWVSRIRNDISHANGLNLWVVSDNVRF",
    "response#3": "KTAVFGATGLVGREIVNLLEEREFPVSDLYLYASESSAGEKMSFSGEDITIKALKPENVENCDIYLSSMPGEVSQQIIPEIKEKNSGVIIDNSSSFRMDRDVPLVVPEVNGEVLDEALARGVKNYIGGNCTVSLMLMGLGPLHEKYRLKRVIISTYQSVTGTGKAAVDQLNAETEFSVNGYKDTESNISKVYPYQIFKNALPHCDVFSDDGYTKEEIKLMKEPKKILGDDSFSLTATAVRVPIQGGHSESVNIEFENEFDLDEVRKILSETPGVVVMDNVEKNEYPMPLYSEGKDDVFVGRMRRDLTQPKTMNLWIVADNLRWGAAEPLRRMLRILLED"
  },
  {
    "reference": "MGPPMKIEDVRRTAYSMPLTNPAFPPGPYRFFDREYFIITYRTDPEALATIVPEPLEVAEPLVKYEFIRMPDSTGFGDYTETGQVIPVRFKGEEGGYTHAMYLDDEAPIAGGRELWGFPKKLARPKIEVESDVLVGSLHYGSVLCASATMGYKHHKVDHDTVLKSMASPNFILKIIPHVDGSPRICELVRFHLDDVVLKEAWTGPAALGLFPHALCDVARLPVREVISALHFKADLTLGLGSVAFDYMAK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have a Schiff-base intermediate with acetoacetate active site that is highly conserved among related enzymes.2. The protein must exhibit the following characteristics: Catalyzes the conversion of acetoacetate to acetone and carbon dioxide.. The designed protein sequence is ",
    "response#1": "RTNTTILEGYAAEPSRQTLSKISNAFKERGTKFDFRVMATHGGTISWKAKELARTIVSGPIGGVIGAKYLGEVLGYKNIACSDIGGTSFDVALITQGEMTIKNDPDMARLVLSLPLVAMDSVGAGAGSFIRLDPYTRAIKLGPDSAGYRVGVCWKESGIETVTISDCHMVLGYLNPDNFLGGAVKLDRQRSVDAIKAQIADPLGLSVEDAAAGVIELLDSDLRDYLRSMISGKGYSPASFVCFSYGGAGPVHTYGYTEGLGFEDVIVPAWAAGFSAFGCAAADFEYL",
    "response#2": "RTNTTILEGYAAEPSRQTLSKISNAFKERGTKFDFRVMATHGGTISWKAKELARTIVSGPIGGVIGAKYLGEVLGYKNIACSDIGGTSFDVALITQGEMTIKNDPDMARLVLSLPLVAMDSVGAGAGSFIRLDPYTRAIKLGPDSAGYRVGVCWKESGIETVTISDCHMVLGYLNPDNFLGGAVKLDRQRSVDAIKAQIADPLGLSVEDAAAGVIELLDSDLRDYLRSMISGKGYSPASFVCFSYGGAGPVHTYGYTEGLGFEDVIVPAWAAGFSAFGCAAADFEYL",
    "response#3": "RTNTTILEGYAAEPSRQTLSKISNAFKERGTKFDFRVMATHGGTISWKAKELARTIVSGPIGGVIGAKYLGEVLGYKNIACSDIGGTSFDVALITQGEMTIKNDPDMARLVLSLPLVAMDSVGAGAGSFIRLDPYTRAIKLGPDSAGYRVGVCWKESGIETVTISDCHMVLGYLNPDNFLGGAVKLDRQRSVDAIKAQIADPLGLSVEDAAAGVIELLDSDLRDYLRSMISGKGYSPASFVCFSYGGAGPVHTYGYTEGLGFEDVIVPAWAAGFSAFGCAAADFEYRTNTTILEGYAAEPSRQTLSKISNAFKERGTKFDFRVMATHGGTISWKAKELARTIVSGPIGGVIGAKYLGEVLGYKNIACSDIGGTSFDVALITQGEMTIKNDPDMARLVLSLPLVAMDSVGAGAGSFIRLDPYTRAIKLGPDSAGYRVGVCWKESGIETVTISDCHMVLGYLNPDNFLGGAVKLDRQRSVDAIKAQIADPLGLSVEDAAAGVIELLDSDLRDYLRSMISGKGYSPASFVCFSYGGAGPVHTYGYTEGLGFEDVIVPAWAAGFSAFGCAAADFEYL"
  },
  {
    "reference": "MEPVDPNLEPWNHPGSQPTTPCTKCYCKKCSYHCLVCFQTKGLGISYGRKKRRQRRSTPPSSEGHQDPISKQPLPQSRGSNSTGSEESKKKVESKTKTDP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design must feature the Nuclear localization signal, RNA-binding (TAR), and protein transduction motif.2. The designed protein's functionality will benefit from Polar residues, Basic and acidic residues compositional bias in its structure.3. A protein that contributes to positive regulation of transcription elongation by RNA polymerase II, suppression by virus of host translation initiation by functioning in host cell cytoplasm, host cell nucleolus, extracellular region with trans-activation response element binding, protein domain specific binding, actinin binding, cyclin binding.4. The protein must exhibit the following characteristics: Tat can reactivate a latently infected cell by penetrating in it and transactivating its LTR promoter; Besides its effect on RNA Pol II processivity, Tat induces chromatin remodeling of proviral genes by recruiting the histone acetyltransferases (HATs) CREBBP, EP300 and PCAF to the chromatin; Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication; This binding increases Tat's affinity for a hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA) and allows Tat/P-TEFb complex to bind cooperatively to TAR RNA; The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive. The designed protein sequence is ",
    "response#1": "MEPVDPNLEPWNHPGSQPKTPCTKCYCKQCSYHCQVCFMTKGLGISYGRKKRRQRRRTPQSSEDHQNSISKQPISQARGDPTGSEESKKKVESKTKTNPFD",
    "response#2": "MEPVDPNLEPWNHPGSQPTTACSKCYCKKCCWHCQICFLKKGLGISYGRKKRRQRRRAPQSSKDHQNPIPKQPLPTTRGNPTGPEESKKKVASKTETDPCA",
    "response#3": "MEPVDPRLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFTKKALGISYGRKKRRQRRRTPQDSETHQVSLSKQSTPQAQGDPTGPKESKKKVERETETDPEN"
  },
  {
    "reference": "MIHLHFGSVPNNCIFDLLCILFTNFNFLLSCRNDTKEDVFVHQTAIKKNNPRKYLRSVGDGETVEFDVVEGEKGAEAANVTGPGGVPVQGSKYAADRNHYRRYPRRRGPPRNYQQNYQNSESGEKNEGAENIPEGQGQQRRPYRRRRYPPYYMRRPYGRRPQYTNPPVQGEIVEGADNQGAGDGRPVRQNMYRGYRPRFRRGPPRQRQPREDGNEEDKENQGDETQSQQPPQRRYRRNFNYRRRRPENPKPQDGKETKTAEPAAENTSAPEAEQGGAE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have at least one CSD domain.2. The protein design should emphasize Polar residues, Basic and acidic residues compositional bias.3. The designed protein should be involved in negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay, negative regulation of striated muscle cell differentiation, CRD-mediated mRNA stabilization, epidermis development, positive regulation of transcription by RNA polymerase II by localizing to endoplasmic reticulum, extracellular exosome, synapse, cytoplasmic stress granule, CRD-mediated mRNA stability complex and exhibiting mRNA binding, sequence-specific double-stranded DNA binding, chromatin binding, miRNA binding, GTPase binding. The designed protein sequence is ",
    "response#1": "AAAAAAALLYGSGGAEDLLATGAPCAACSAASCANNAFAFGPELSSLITPLAIQTHNFAAAAAAAAYYRSQQQGLAPPAQPPLPRRLSESPVFDLPERLLELI",
    "response#2": "AGLEAVLPNAYCNAVIQVLYYIEPLRASLLSHLCSKEFCLSCELGFLFYMLSHSPPNQPCQPGNFLRAFRTVPEASALGLILSDLAPEAKQKTDLGALIQSWNRFMLHQMHVELNETRKRNEEKKEKFCKPFIYKEIDFPAISKERARKHSGEYSLSAAEVSNEIEEEKREDVSDISNIFGSKQMHRHSCLKCAEEVNKGSILLACNLSLPKVLSINTGLHTPQHKQFWQTQMDKVVAKLI",
    "response#3": "DRHVMAKHSVVYPTQEELEAVQNMVSHTERALKAVSDWIDQQEKDCSGEQEQPMAEETETTEEGKDSEMKTGENPTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCRDKPTISLLKRVADNLVLQFETVSEDKYEVIQNIREALIVVKSTKEPPLTLNIRLTSPLVREEMEKLSAGETLTVSDPPDVLDRHKCLAALASLRHAKWFQARAR"
  },
  {
    "reference": "MKQKKFPFNTVEEAIEDIRQGKMVIVVDDEDRENEGDLVMAAEKVTPEAINFMATYGRGLICVPLIGERLDELDIQPMVTHNTDNHETAFTVSVDSKYTTTGISAHERALTVKALLDSNTRPQDLRRPGHIFPLRAKDGGVLRRAGHTEAAVDLARLAGLYPAGVICEIMNDDGTMARVPQLINFARKHQLKIITIADLIEYRRRTEKLVRRVENTRLPTRYGDFIAVAYENILDGECHIALVKGEIDGREPVLVRVHSECLTGDVFGSRRCDCGEQLAAAMQMIEREGKGVLLYMRQEGRGIGLVNKIRAYKLQDEGKDTVEANEALGFPADLRDYGIGAQILVDLGIREIKLLTNNPRKIAGLEGYGLRVVERIPLEISPNANNRRYLVTKKNKLGHLLNVN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must be able to manganese ion binding, zinc ion binding, GTP cyclohydrolase II activity, magnesium ion binding.2. The Nucleophile; for GTP cyclohydrolase activity active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..4. The designed protein should have a high specificity for GTP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "RVAETKLPTQYGEFKAVAYASIIDSDEHVVLVKGDISTNDPVLVRVHSQCLTGDTFGSQRCDCGEQIHRSLEAIQAEGRGVFLYMRQEGRGIGLHNKLRAYQLQDAGLDTVEANEKLGFPADLRWYGIGAQILVDLGIKRIRLLTNNPKKVVGLDGYGLEIMERVG",
    "response#2": "EAVTRLPTRHGDFRAYGYRSTTDGVEHVALVAGELGDGADVLVRVHSECLTGDVFGSLRCDCGPQLQESLARVQAEGRGVVLYLRGHEGRGIGLLPKLRAYELQESGHDTLDANLALGLPADARDYGAAARILTDLGVRSLRLMTNNPDKTDALLRHGLRVEGRP",
    "response#3": "VSVELPTRIGDFQLKAYRQTTTEMEHLALIKGSWEPDEPVLVRVHSSCMTGDIFGSCRCDCGPQLQKSMEMIEKEGKGVIVYMNQEGRGIGLVNKLKAYKLQEDGMDTVEANLELGFKMDQRDYGVGAQILRDLNVGKMRLLSNNPKKRTGLIGYNLEIVENA"
  },
  {
    "reference": "MMRPILIGITGGTGSGKSTVANEIYESFKDDCIAIIEQDSYYKDQSHLTFEDRIKTNYDHPNAFDTELLVEHLKELSKGNTINKPIYDFKEHTRKKEVVEVKAKDIIIVEGIMILQDVELRNLLDIKIYVDTDDDVRIIRRILRDIKERGRTIDSVVDQYLGVVKPMHSQFIEPTKKYADIIIPEGGQNKVAIDIMVSKIKQILSENK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. We need a protein that can perform ATP + cytidine = ADP + CMP + H(+) with high efficiency.2. A protein that contributes to UMP salvage, CTP salvage by functioning in cytoplasm with phosphotransferase activity, alcohol group as acceptor, ATP binding.3. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.4. The protein should be optimized for activity in UMP biosynthesis via salvage pathway, UMP from uridine: step 1/1 pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "VIGVTGGSGSGKTTVSNAIYNQLSGQSLLILQQDSYYNDQSEMTMAERHAVNYDHPLAFDTDLMIKQIKQLLAYQPIEKPVYDYEQYTRSDKTIHQEPRDVIIVEGVLILDDQRLRDLMDIKVFVDTDDDIRIIRRIQRDIKERGRTLDSVIGQYLATVKPMYHQFVEPTKRYADLIVPEGGENEVL",
    "response#2": "VIGIAGGSGSGKTSVTNSIYEKFKGHSILMLQQDLYYKNQSHMPFEECLLTNYDHPLAFDNDLMFEHLEQLLRYESIEKPIYDFKLNTRSEETVHVEPKDVIIVEGIFALEDERLRDLMDIKLYVDTDADLRIIRRILRDTKERGRSIDSVIEQYVSVVRPMHNQFIEPTKRYADIIIPEGGQNHL",
    "response#3": "VIGVTGGSGSGKTTVSNAIYNQLSGQSLLILQQDSYYNDQSEMTMAERHAVNYDHPLAFDTDLMIKQIKQLLAYQPIEKPVYDYEQYTRSDKTIHQEPRDVIIVEGVLILDDQRLRDLMDIKVFVDTDDDIRIIRRIQRDIKERGRTLDSVIGQYLATVKPMYHQFVEPTKRYADLIVPEGGENEVLQGGAG"
  },
  {
    "reference": "MNPLMFTLLLLFGFLCIQIDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The Extracellular topological domain of the protein should be designed to be flexible or rigid.3. For general function, the protein need meet that Receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.4. The designed protein must have at least one Fibronectin type-III, Ig-like C2-type domain.5. A protein that can perform identical protein binding, axon guidance receptor activity in cell surface, axolemma, plasma membrane to support metanephros development, homophilic cell adhesion via plasma membrane adhesion molecules, olfactory bulb interneuron development, positive regulation of axonogenesis.6. The protein should exhibit Polar residues, Acidic residues, Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "LDFQRFLTLNRTLLVAARDHVFSFDLQAEEEGEGLVPNKYLTWRSQDVENCAVRGKLTDECYNYIRVLVPWDSQTLLACGTNSFSPVCRSYGITSLQQEGEELSGQARCPFDATQSNVAIFAEGSLYSATAADFQASDAVVYRSLGPQPPLRSAKYDSKWLREPHFVQALEHGDHVYFFFREVSVEDARLGRVQFSRVARVCKRDMGGSPRALDRHWTSFLKLRLNCSVPGDSTFYFDVLQALTGPVNLHGRSALFGVFTTQTNSIPGSAVCAFYLDEIERGFEGKFKEQRSLDGAWTPVSEDRVPSPRPGSCAGVGGAALFSSSRDLPDDVLTFIKAHPLLDPAVPPVTHQPLLTLTSRALLTQVAVDGMAGPHSNITVMFLGSNDGTVLKVLTPGGRSGGPEPILLEEIDAYSPARCSGKRTAQTAPTNQTLPKGSVATMPCRATGNPTPRVKWFHDGYAVQTTSRHSIVQGSSLRVDDLQLSDSGLYTCTASSERGESSWSAALMVLIKGPPYFMKEPKDLTMVYGQTATFHCSVGGDPPPKVLWKKEEGNIPVSRARILHDEKSLELTNITPNDEGTYVCEAHNNVGQISARATLTTGKVVELIDASAVNASAVRLDWMLHVSENEKFVEGLHIRYRDYSSHSTLYNVITVLDASVESHVVGNLRKYTKYEFFLTPFYETIEGQGMPGPPEDVQVEDISSTTSQLSWRAGPDNNSPIQIFTIQTRTPFSVGWQAVATVPEILNGKTYNATVVGLSPWVEYEFRVVAGNSIGIGEPSEPS",
    "response#2": "PVATVGHVVLPCSPSSAWASCVWHQPSGVTSLTPRRDGLEVVVTPGAMGAYACECQEGGAARVVAAYSLVWGSQRGPANRAHTVVGAGLVGFFLGVLAASLTLLLIGRRQQRRRQRELLARDKVGLDLGAPPSGTTSYSQDPPSPSPEDERLPLALGKRGSGFGGFPPPFLLDSCPSPAHIRLTGAPTAKLSVIFLDSASVQLLGSNRNELLLKCHVEGASGNEALQIEWYRNSAKLSSWQNIELDEHRLLVRQPTGSDDGLYRCIASNAAGRVMPTNKKIGLNGQVQLPCVATGNPPPSVFWTKEGVSTLMFPNTSHGRQHISAEGTLQINDVQQEDEGFYVCSAFSVVDSSTVRIFLQVPEWEQKIQNTHLSIYDNLLWECKASGKPNPWYTWLKNGERLNPEERIQIENGTLIITMLNVSDSGVYQCAAENKYQIIYANAELRSTVSVREGQGVVLLCGPPPHFGDLSYAWTFNDNPLYVQEDNRRFVSQETGNLYIAKVEPSDVGNYTCFITNKEAQRSVQGPPTPLVQWKGS",
    "response#3": "LEDVFLLQTRDNKNPVIFGLFSTTSNIFRGYAICVYHMAIVRAAFNGPYAHKEGPEYYWALYEGKVPYPRPGSCASKVNGGLYTTTKDYPDEAVHFARSHPLMYQPIKPVHKRPILVKTDGKYNLKQIAVDRVEAEDGQYDVLFIGTDNGIVLKVITIYNQETESMEEVILEELQVFKVPIPILSMEISK"
  },
  {
    "reference": "MSPTEKRTADARHAPSDGAQTTDFGYQDVPRHEKASKVADVFHSVAARYDVMNDLMSMGIHRLWKRLTIERSGVRPGHRVLDIAGGTGDLTLKFSRMVGPRGQVVLADINASMLGVGRDKLIDNGVGGNVDYVQANAECLPFPNNSFDCITIAFGLRNVTDKDAALRSMQRVLKPGGRLLVLEFSKPQSALLTKAYDEYSFRFLPRIGQLVAGDADSYRYLAESIRMHPDQPTLKGMMEAAGLERVEYTNLTGGIVALHRGIKL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must be able to S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity, 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity.2. The protein should be able to bind S-adenosyl-L-methionine ligand in a variety of conditions3. Ideal protein should have a binding affinity for key enzymes in the menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2 pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MNLLLLGAPGAGKGTQAKFLEKKYNIPHISSGDTFR",
    "response#2": "MSSLSNKVAIVTGSGRGIGKAIALGLAKEGANVVVNYSNS",
    "response#3": "MTVLPPENQPG"
  },
  {
    "reference": "MMKKIVFATNNDHKLEEVRYKLSSQYEILSLKDINCHDDIPETANTFTGNAEQKAVWVLEKYGYDCFADDSGIEIEALDNRPGVFSARYAGENCSFDDNNNKVLQELEGVENRKATFSTTICLKLKGKTHFFVGDIVGDIISDYQGDKGFGYDPIFVPKGYDRSFAQMSLEEKNKISHRAIAVENMWNFLNKM",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The Mg(2+), substrate binding site should be stable and able to withstand changes in environmental conditions.2. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP4. The designed protein must be able to perform ribonucleoside triphosphate phosphatase activity, ITP diphosphatase activity under specific conditions. The designed protein sequence is ",
    "response#1": "MPPRRGGRR",
    "response#2": "MKLIFATHNRGKAKEFAELLGGKHDIELLTMDELGIHDDIPETGETLEANAEAKARYVTEHYGLDCFADDTGLEIEALNGAPGVYSARYAGENRTDEANIELLLKALEEVPPEKRTARFRTVIALMLPDRKEHLFEGICEGAICKTPRGTGGFGYDPVFVPEGYDRTFAEMSLEEKNAISHRGRALRKMVDFLEK",
    "response#3": "MVSSTPLVVASGNEHKLRELRSLL"
  },
  {
    "reference": "MPPKSRSTGPKKTQKTRRRDKKNIPHGAAHIKSTFNNTIVSITDPAGNVISWASSGHVGFKGSRKSTPFAAQLAAESAARKAQEHGVKKVDVFVKGPGSGRETAIRSLQAAGLEVGTISDVTPQPHNGCRPPKRRRV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have structural constituent of ribosome within ribonucleoprotein complex to regulate translation.2. The protein must exhibit the following characteristics: Forms part of the Shine- Dalgarno cleft in the 70S ribosome.; Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. The designed protein sequence is ",
    "response#1": "DSFKK",
    "response#2": "MIIGCMVTLRGDRMFEFFDRLVNVALPRVRDFRGVSSKSFDGRGNYTLGIKDQLIFPEIDYSKVDKTKGMNICITTTARTDAEGLALLRTMGMPR",
    "response#3": "MPRKGNVQKREVLPDPIYNSKLVTRLINHMMMDGKRGTSTKIIYGAFDIIKNETGNDPVETFEEAMKNIMPVLEVKARRVGGSNYQVPIEVRPDRRTTLGLRWLVQYSRLRGEHTMVERLAREIMDAANNTGAAVKKREDTHRMADANR"
  },
  {
    "reference": "MADSITTIVTALGFPSIESFIGVLLLGGAIIGAIVVIATIRPILDLFPFAYPNARVRARIGRLLNEKQLSEILETESMEEFKNYLRGLPDYAKYIDRFPIEKALESQLAETYEMVSQIAPASIRDPFRANLKRWDVRNIKSLITAKAAGLSAEETVNLLVPGGEVYEIIEGLADASSVQEVVTGLEATEYAGVLEDALSGYEETGMLLPIEAALDRKFLEGLIRTVGSPSDDNTKILHTYFGTMVDISNLKIILRAKADGLSYDDISPYIVPHGYQIREWKLKDLMESEDVSGVVSGLEGTDYGQMLSEALSEYTSTGSVAVFERVLEDNLNRMARNFALKKPFGVGPMIGFLSRKEVEVKNLKVIARSKREPGFPEAMVKEMLA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Produces ATP from ADP in the presence of a proton gradient across the membrane.2. A protein with proton-transporting ATP synthase activity, rotational mechanism is needed to enhance proton motive force-driven plasma membrane ATP synthesis in a specific context. The designed protein sequence is ",
    "response#1": "GISSIDHLNTLVRGQKLPVFSGSGLPHNELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLANDPAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYPGYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHELYNQGYRPPINVLPSLT",
    "response#2": "VNPLDRDREWTVSVKVKLGDRVKPGQVVAEVPETSIVTHRVMVPPHLSGEVVEVVPNGKYTVDQEIVTVKDDKGNNHQIRLHQKWPVR",
    "response#3": "GQRVFDTFFPLGMGGTAAIPGGFGAGKTMTQHQLAKWSSADIIVYVGCGERGNEMTDVLEEFPKLEDPSTGKSMMERTVLIANTSNMPVAAREASIYTGITIAEFYRDMGYNVALMADSTSRWAEALREISGRLEEMPAEEGFPAYLPSRLAEFYERAGYVKTLGQDREGSISLIGAVSPPGGDFSEPVTQNTKRYVRCFWALDKSLASARHFPAVNWLESYNVNPLDRDREWTVSVKVKLGDRVKPGQVVAEVPETSIVTHRVMVPPHLSGEVVEVVPNGKYTVDQEIVTVKDDKGNNHQIRLHQKWPVN"
  },
  {
    "reference": "MSDFRQDFIRFALDKQVLKFGEFITKAGRKSPYFFNAGLFNDGLSTLNLSRFYAKSIQQSGIQFDMLFGPAYKGIILAAAAGMALAEQGRNVPFAYNRKEAKDHGEGGTLVGAPLKGKVLIIDDVISAGTSVRESVELIRAAGAEPAGVAIALDRMERGQGELSAVQEVAQQHGLPVVAIATLKDLLGFLENSPELAAHLDAVRAYRAQYGVDD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Mg(2+) is required for the proper folding and stability of the protein.2. The protein should be optimized for activity in UMP from orotate: step 1/2, UMP biosynthesis via de novo pathway pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The designed protein should be able to compete with endogenous proteins or inhibitors for 5-phospho-alpha-D-ribose 1-diphosphate, orotate binding.4. The designed protein should be optimized for orotate phosphoribosyltransferase activity, magnesium ion binding activity in order to improve 'de novo' UMP biosynthetic process. The designed protein sequence is ",
    "response#1": "CLKLIAEAFAERVADDDKLAGVALGAVPLVAATAVETNKPYVIARKKAKEYGTAKRIEGALDEGEEVVVLEDIATTGQSAVDAVEALREAGAVVDRVVVVVDRQEGAAELLADHDIALESLLS",
    "response#2": "LLKIEAVRLRPEEPFKWASGWNSPIYCDNRLTLSFPESRNLIRDGLVDLIRTHYPACECIAGVATAGIPQGAIIADSMNLPMIYVRPKPKDHGMQNLIEGKITKGQKIVVVEDLISTGGSSLKAVEALQEGGFEILGMIGIFTYGFQIAEDNFKNAGITLHTLSNYDC",
    "response#3": "MLESGAILFGHFVLSSGKESDYYINVKKVVTDPDALELMAGLIAGLVQRVGIDFDRVAGPELGAVPIATAVSLKTGKPLVIVRKKPKGYGTGSQIEGDVKEGDRILLVEDVTTTGGSVLRAAEVLESLGARIAAIAVVVDREEGAEGSITSKGYTFLPVIRVSE"
  },
  {
    "reference": "MLSSLIRDSFPLLILLPTFLAALGASVAGGVMGTYIVVKRIVSISGSISHAILGGIGLTLWIQYKLHLSFFPMYGAIVGAIFLALCIGKIHLKYQEREDSLIAMIWSVGMAIGIIFISRLPTFNGELINFLFGNILWVTPSDLYSLGIFDLLVLGIVVLCHTRFLALCFDERYTALNHCSVQLWYFLLLVLTAITIVMLIYVMGTILMLSMLVLPVAIACRFSYKMTRIMFISVLLNILCSFSGICIAYCLDFPVGPTISLLMGLGYTASLCVKKRYNPSTPSPVSPEINTNV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should be able to enhance the efficiency of transmembrane transport. The designed protein sequence is ",
    "response#1": "QSFWLITGIIFIAFNLRPAITSVGPVISSIRAELHMSNGAAGFLTALPLLSFAVLSPLAPKLGQRLGNERTLWLGLVILLIGVLTRSTGYTAALFFGTALIGVGIAIGNVLLPSLIKHKYPEKPGIMISLYTTSMNIFAALASGVSVPLATQMNGGWKQAFLLWGGLALLALLIWIPQLRHRDTANQTMKLQSSSIWASKMAWYVTIFMGLQSFLFYSSIAWFPEILRSHGIDTATAGWMVSLMQFASLPSTFLTPVLADRVKQQRGIVAALASVYLIGLCGLLAGGSHTLLAIWMIIIGIGQGSSISLALTLIGLRSENAQQAAALSGMSQSFGYLLAAVGPIFVGYLFDQTHSWTMPIVLLIAALIVMGAAGQGAGRDRYR",
    "response#2": "QSFWLITGIIFIAFNLRPAITSVGPVISSIRAELHMSNGAAGFLTALPLLSFAVLSPLAPKLGQRLGNERTLWLGLVILLIGVLTRSTGYTAALFFGTALIGVGIAIGNVLLPSLIKHKYPEKPGIMISLYTTSMNIFAALASGVSVPLATQMNGGWKQAFLLWGGLALLALLIWIPQLRHRDTANQTMKLQSSSIWASKMAWYVTIFMGLQSFLFYSSIAWFPEILRSHGIDTATAGWMVSLMQFASLPSTFLTPVLADRVKQQRGIVAALASVYLIGLCGLLAGGSHTLLAIWMIIIGIGQGSSISLALTLIGLRSENAQQAAALSGMSQSFGYLLAAVGPIFVGYLFDQTHSWTMPIVLLIAALIVMGAAGQGAGRDRYR",
    "response#3": "QSFWLITGIIFIAFNLRPAITSVGPVISSIRAELHMSNGAAGFLTALPLLSFAVLSPLAPKLGQRLGNERTLWLGLVILLIGVLTRSTGYTAALFFGTALIGVGIAIGNVLLPSLIKHKYPEKPGIMISLYTTSMNIFAALASGVSVPLATQMNGGWKQAFLLWGGLALLALLIWIPQLRHRDTANQTMKLQSSSIWASKMAWYVTIFMGLQSFLFYSSIAWFPEILRSHGIDTATAGWMVSLMQFASLPSTFLTPVLADRVKQQRGIVAALASVYLIGLCGLLAGGSHTLLAIWMIIIGIGQGSSISLALTLIGLRSENAQQAAALSGMSQSFGYLLAAVGPIFVGYLFDQTHSWTMPIVLLIAALIVMGAAGQGAGRDRYR"
  },
  {
    "reference": "MRVRGIQMNWLNLWRWGILSIGLVIMCSASNNLWVTVYYGVPAWKDADTTLFCASDAKAHETEAHNVWATHACVPTDPNPQEIPLENVTENFNMWKNKMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTNANLPQNTTNGNISIGNITDEVKNCTFNMTTEIRDKQQIVHALFYTLDIVQMEGNGSKYRIINCNTSVIKQACPKISFNPIPIHYCAPAGYAIIKCKDKKFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENLTDNTKTIIVHLNKSVTINCTRPSNNIRTSTRIGPGQVFYRTGAITGDIRKAYCEVNGTEWNKTLDQVVKKLKEHFNKTIVFRPPSGGDLETTMHHFNCRGEFFYCNTTKLFNSTGNGTIILPCKIKQIVRMWQGVGQAMYAPPIEGKINCISNITGILLTRDGGINNTTDETFRPGGGNIKDNWRNELYKYKVVQIEPLGIAPTRAKRRVVDREKRAVGIGAMIFGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSNKVICTTNVPWNSTWSNKSYDEIWENLTWIEWEKEISNYTSIIYDLLTESQNQQEKNEKDLLELDKWASLWNWFSISNWLWYIKIFIIIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTPSHQQREPDRPEGIEEEGGEQGRDRSTRLVSGFLALAWDDLRSLCLFSYHRLRDFILIATRTVELLGK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; Transmembrane protein gp41: Acts as a class I viral fusion protein2. Include a signal peptide as an essential component of the protein.3. The designed protein should have structural molecule activity within virion membrane, viral envelope, host cell endosome membrane to regulate positive regulation of receptor clustering, clathrin-dependent endocytosis of virus by host cell.4. The designed protein should contain at least one YXXL motif; contains endocytosis signal motif to achieve its intended function.5. The protein should exhibit Basic and acidic residues compositional bias.6. The designed protein must have at least one Retroviral envelope protein GP41-like domain. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWRDAQTSLFCASDAKAYDTEVHNVWATHACVPTDPNPQEMVLKNVTENFDMWKNNMVDQMHQDIISLWDQSLKPCVKMTPLCVTLNCTNATTAMLWVTVYYGVPVWREATTTLFCASDAKAYSQEVHNVWATHACVPTDPNPQEMVLENVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLSCSNVTVNCNSSTVNCPNDTNNNVAGEMKNCSFNMTTELRDKRKQEYSLFYRLDIVPLNKNNSSTSNYSDYRLINCNTSAVTQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEKDIVIRSENLTNNAKTIIVQLKEPVKIVCTRPNNNTRKSMRIGPGQTFYATGEIIGDIRHAHCNITEANWNKTLQEVGKKLGEHFPNRTIQFAPSSGGDLEIKTHSFNCRGEFFYCNTTKLFNSTYNSNGTYSNSTSNITLQCRIKQIINMRQEVGRAMYAPPIAGNITCESNITGLLLTRDGGNGSSDAPEIFRPGGGNMKDNWRSELYKYKVVEIKPLGVAPTTAKRRVVEREKRAVGLGMLFLGVLSAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQQLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNKSQSDIWDNMTWMQWDREXINYTXQIYXLLEQSQNQQEKNEQDLLALDKWXNLWNWFNISNWLWYIKIFIMIVGGLIGLRIVFAVLSIINRVRQGYSPLSL",
    "response#2": "MKNCSFNATTELRDKKRKVHALFYKLDVVQLGGNNSNTTYRLINCNTSAITQACPKVTFDPIPIHYCAPAGYAILKCNNQTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVIIRSENLTNNAKTIIVHLNESVTITCARTNNNTRESIRIGPGQTFYATGDIIGDIRQAHCNITKGAWNKTLQQVGEKLAKLFPNKTIKFNSSSGGDLEIATHSFNCRGEFFYCNTSKLFNGEYRPNYSNYTDNSTITLQCRIKQIINMWQEVGKAMYAPPIAGNITCVSSITGILLTRDGGDINSSIETFRPGGGDMRDNWRSELYKYKVVEVKPLGIAPTGAKRRVVERERRXRLGALXVQARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTTVPWNASWSNKSLEKIWGNMTWMQWEQEIDNYTGLIYTLLEESQNQQEKNEQELLELDRYAATGTASTNQVSLWVTVYYGVPVWRDAETTLFCASDAKAHETEVHNVWATHACVPTDPNPQEIHLENVTEDFNMWKNKMADQMQEDVISLWDQSLEPCVKLTPLCVTLNCTDALLKNETKNN",
    "response#3": "MKNCSFNATTELRDKKRKVHALFYKLDVVQLGGNNSNTTYRLINCNTSAITQACPKVTFDPIPIHYCAPAGYAILKCNNQTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVIIRSENLTNNAKTIIVHLNESVTITCARTNNNTRESIRIGPGQTFYATGDIIGDIRQAHCNITKGAWNKTLQQVGEKLAKLFPNKTIKFNSSSGGDLEIATHSFNCRGEFFYCNTSKLFNGEYRPNYSNYTDNSTITLQCRIKQIINMWQEVGKAMYAPPIAGNITCVSSITGILLTRDGGDINSSIETFRPGGGDMRDNWRSELYKYKVVEVKPLGIAPTGAKRRVVERERRXRXLGPGXVMGAAAVTLTVQARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLRDQQLLGLWGCSGKLICTTSVPWNSSWSNKSLEEIWQNMTWMDWEKEINNYTNLIYNLIEESQNQQEKNEQDLLALDKWASLWNWFDISKWLWYIKIFIMIVGGLIGLRIVCTVFSIVNRVRQGYSPLSFQTXLPARKXDXXG"
  },
  {
    "reference": "MGSLLGLCLIVQIITGLFLAMHYVSDISSAFSSVAHICRDVNYGWLIRNFHANGASLFFICIYLHIARGLYYGSYLYMETWNIGVILLLLTMMTAFVGYV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should contain a Helical membrane-spanning region.2. The protein must bind to heme b in order to perform its enzymatic function.3. The protein should be able to bind heme b ligand in a variety of conditions4. The protein must exhibit the following characteristics: The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c; Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.; Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain.5. The protein should have metal ion binding, electron transfer activity and be active in respirasome, mitochondrial inner membrane. The designed protein sequence is ",
    "response#1": "VAYPTPMNLNYSWNGGSLAGMMLASQMLTGILLAMHYVGHVDYAFASVQHLMTDVPSGMILRYAHANGASLFFIVVYLHVLRGMYYGSGAQPREIVWISGVVILLVMIITAFIGYVLPWGQMSFWGATVITSLATAIPVVGKHIMYWLWGGFSVDNPTLNRFYSFHYTLPFILAGLSVFHIAALHQYN",
    "response#2": "MFWLCLVCLSVSHKMIGLCYLLVAILSGFVGYVYSLFIRLELSLIGCGILFGDYQFYNVLITSHGLIMVFAFIMPVMMGGLVNYFIPVMAGFPDMVFPRLNNMSFWMYLAGFGCVVNGFLTEEGMGVGWTLYPTLICIDFHSSLACDFVMFAVHLLGISSILNSINLLGTLFCCRRKFFSFLSWSLFIWAALITAILLIITLPVLAGGVTLILCDRNFNTSFYDVVGGGDLILFQHIFWFFGHPEVYIILLPVFGLISTIVEVIGFRCVFSTVAMIYSMILIAILGMFVWAHHMFVVGMDVDSRAYFGGVSILIGLPTCVKLFNWIYSFLYTDMIITFEVYFVIMFIFMFLIGAVTGLFLSNVGIDIMLHDTYFVVGHFHYVLSLGAVVGFFTGFIHFLAKWLPIELYLFWMFYFISTLFIGSNMLFFPMHSLGMYAFPRRISDYPVSFLFWSSFMLYGMLLLASLILFLCALFCVFLFWDYCLFFVSLFVFSLFSNYFFN",
    "response#3": "MFWLCLVCLSVSHKMIGLCYLLVAILSGFVGYVYSLFIRLELSLIGCGILFGDYQFYNVLITSHGLIMVFAFIMPVMMGGLVNYFIPVMAGFPDMVFPRLNNMSFWMYLAGFGCVVNGFLTEEGMGVGWTLYPTLICIDFHSSLACDFVMFAVHLLGISSILNSINLLGTLFCCRRKFFSFLSWSLFIWAALITAILLIITLPVLAGGVTLILCDRNFNTSFYDVVGGGDLILFQHIFWFFGHPEVYIILLPVFGLISTIVEVIGFRCVFSTVAMIYSMILIAILGMFVWAHHMFVVGMDVDSRAYFGGVSILIGLPTCVKLFNWIYSFLYTDMIITFEVYFVIMFIFMFLIGAVTGLFLSNVGIDIMLHDTYFVVGHFHYVLSLGAVVGFFTGFIHFLAKWLPIELYLFWMFYFISTLFIGSNMLFFPMHSLGMYAFPRRISDYPVSFLFWSSFMLYGMLLLASLILFLCALFCVFLFWDYCLFFVSLFVFSN"
  },
  {
    "reference": "MKTVSLEKTQRRIGSMFDRIAATYDFLNHFLSAGLDLWWRRRAVAALSVPAGGVILDVATGTGDLAFAALKAHEGVRVVGLDLAFNMLRIAKAKREKKGTPPRRYQLLRGDALELPFAAESFDAVMIAYGIRNVPDMGAALEEFHRVLRPGGTLLVLEFSLPSAALLKRLYLFYFEKILPRLGGAVSRDPEAYAYLPASVGAFVTPEDLGGLARGRGFHRLEVRRLTGGVTYYLRAVRDSR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should be able to enhance the efficiency of aerobic respiration, menaquinone biosynthetic process.2. For general function, the protein need meet that Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2). The designed protein sequence is ",
    "response#1": "MTTPS",
    "response#2": "MKNH",
    "response#3": "MCPGGKSKL"
  },
  {
    "reference": "MGGKWSKSSIVGWPQVRERMRRTPPAAIGVGAASRDLDKHGAITSSNTASTNAACAWLEAQEEEEVGFPVKPQVPLRPMTYKGAVDLSHFLKEKGGLEGLIHSQQRQDILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPMDPEEVEKATEGENNCLLHPVCQHGVEDADREVLVWKFDSSLAFKHGALELHPEYFKN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in host cell Golgi membrane that is critical for suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host autophagy, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II.2. The protein must exhibit the following characteristics: Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis.3. The protein's functional design necessitates the inclusion of a Diacidic; necessary for CD4 internalization motif. The designed protein sequence is ",
    "response#1": "MGGKWSKRSVVGWPAVRDRIRQTPPAAAGVGAVSRDLEKHGAITSSNVNNADCVWLRAQEEEEEEVGFPVRPQVPLRPMTFKGAFDLSFFLKEKGGLDGLIYSRKRQEILDLWVYHTQGFFPDWQNYTPGPGIRYPLTFGWCYKLVPVDPREVEEANEGENNSLLHPMCQHGMEDEEGEVLMWKFDSSLARRHVARELHPEFYKDC",
    "response#2": "MGGKWSKSSIVGWPAVRERIRRTEPAAEGVGAASRDLEKHGALTSSNTPANNAACAWLEAQEDEEVGFPVRPQVPLRPMTYKGAFDLGFFLKEKGGLDGLIYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPREVEENNKGENNCLLHPMSQHGMEDEDREVLMWKFDSSLARRHIARELHPEYYKDC",
    "response#3": "MGGKWSKRSMAGWPAVR"
  },
  {
    "reference": "RPPGFSPFR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Induces relaxation of mouse trachea that has been precontracted with methacholine; May target bradykinin receptors (BDKRB).; May induce relaxation of arterial smooth muscle2. A protein that can perform toxin activity in extracellular region to support defense response, vasodilation. The designed protein sequence is ",
    "response#1": "LPHHHHHH",
    "response#2": "LAGL",
    "response#3": "SAXFXDXC"
  },
  {
    "reference": "MTATADLWPLFPDHIKQLQTRAERLFKRENLDLVAIHSGQQKRWFLDDMNYPFRANPHFKAWCPETQLANAWVILKPNTRPTLVLLSSPDFWHTTASLEGAPWLEEFHVEHISSPEAIEKLLPYDKKSAAYLGEHIEVAKALGFENINPDPVLHFFHYHRLFKTDYEIACLTQANHIAAEGHVAAADAFFNGASEFDCLLKYMAATRQGQNEVPYNHIIGQNENASVLHHWMPDKKASGSLKSMLVDAGAEVCGYAADISRTWSKQHNEYEELIAALDQITLALIDKMKPGVEFPALHQLAHEQIANVLFAFGFVSCSPEQMIEDGITTVFLPHGLGHPLGLQVHDVGAAQADERGTPIAPPSGHLTLKTTRTVEPRQVYTIEPGIYFIEPLLQKLANSRNKHLINWRRVDEFKPFGGVRIEDNIVVYRERNDNLTRQTALDAYVKKVTRLA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have a binding site that is specific to the target Mn(2+) ligand.2. The desired protein should be able to perform Hydrolysis of Xaa-|-Pro dipeptides. Also acts on aminoacyl-   hydroxyproline analogs. No action on Pro-|-Pro.3. A protein with phosphoric triester hydrolase activity, proline dipeptidase activity, metal ion binding functions is required to enable proteolysis. The designed protein sequence is ",
    "response#1": "LQHAMDACRVIKDEHESEAQIAGLFIDVCLSLRSRGTAYQTIAASGPNGATLHYTRNNEPLAGRQMVVLDAGAEWDCYASDVTRSFPIPSSVSGKENSGKGDWPSREAEEVYAIVQRMQEESISRVREGAMFFSIHQHAHSIALDELLRLGILRIPHGSSKADLVKAEATALFFPHGLGHHLGLEVHDVAPDSGTVPVTSSSSSSSSRVTAVTAQCQDGLMSVTEHRPPCTLSAPPLAAGMVITVEPGIYFNRLAIDQARAERDKPGSKGRFVDFDVVERYMAVGGVRIEDDVLVTE",
    "response#2": "ECMRRANQIAVQGHQAAKNAFYNGASEFEIQQQYLSAVGQGENEVPYGNIIALNQNAAILHYTALEHQNPARRLSFLIDAGASYFGYASDITRTYAFEKNRFDELITAMNKAQLELIDMMRPGVRYPDLHLATHGKVAQMLLDFDLATGDAQGLVEQGITSAFFPHGLGHMLGLQVHDVGGFAFDERGTHIPAPEAHPFLRCTRILAPNQVLTMEPGLYIIDSLLNELKQNSRGKQINWNSVDELRPFGGIRIEDNVVVSRG",
    "response#3": "LQHAMDACRVIKDEHESEAQIAGLFIDVCLSLRSRGTAYQTIAASGPNGATLHYTRNNEPLAGRQMVVLDAGAEWDCYASDVTRSFPIPSSVSGKENSGKGDWPSREAEEVYAIVQRMQEESISRVREGAMFFSIHQHAHSIALDELLRLGILRIPHGSSKADLVKAEATALFFPHGLGHHLGLEVHDVAPDSGTVPVTSSSSSSSSRVTAVTAQCQDGLMSVTEHRPPCTLSAPPLAAGMVITVEPGIYFNRLAIDQARAERDKPGSKGRFVDFDVVERYMAVGGVRIEDDVLVTE"
  },
  {
    "reference": "MNSYVNMGVGALLGTVFVLMSVSIASEGIFHAPAPEKEGYTIVAAESGGGEAGGGEAAAAATPIAKLLASADAAKGEAVFKKCTSCHTGESGGANKVGPNLFDVVNRPIASHEGFSYSAGMKDFSKGASVHWDYDHLNYFLEAPKKHVPGTAMGFAGVKKETERADLIAYLRSLSANPAPLPDPNAAPAPMN",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The heme c binding site should be stable and able to withstand changes in environmental conditions.2. The protein should have electron transfer activity, heme binding and be active in periplasmic space, plasma membrane.3. For general function, the protein need meet that May be involved in electron transfer from bc1 complex to aa3.4. The designed protein should contain Periplasmic, Cytoplasmic topological domains.5. The protein should contain Helical; Signal-anchor transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "HQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFAPADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAVFTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAGILVLIGVVNLPIIHYSVEWWNTLR",
    "response#2": "HQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFAPADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAVFTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAGILVLIGVVNLPIIHYSVEWWNTLR",
    "response#3": "LFILAFFTGGALTLYAARASEMQAKGLFSMVSRESALVMNNVLLAVAALVVFTGTVWPLIAELFWDRKLSVGAPFFEKAFTPFMVGLALLLPLGSMMPWKRASLGKLVRPLLPALVLTLAVLALVWVMATGRPMLALGAAGLGAWILFGALAEIWQRAGRTPGRILRLPRADWGKAFAHGGLGIVFAGVGLLMAGQVEDIRVAKAGDSFEVAGYTITLVSVEDVPGPNFTAKTATMEVRQGGKLVATLHPEKRIYPVQAMPTTEADIDNGFWRDVYLVIGDPQEGGGWAVRTYVKPFANWIWAGCLLMAFGGGLSLTDMNTATSTAYSYKVVRQFAIMTVVWGIVGMGLGVFIAAQLAWPFLNFDLPWTSFGRLRPLHTNAVIFAFGGCALFATSYYSVQRTCQTTLFAPKLAAFTFWGWQLVILLAAISLPLGFTSSKEYAELEWPIDILITIVWVAYAVVFFGTLAKRKVKHIYVGNWFFGAFILTVAILHVVNNLEIPVTAMKSYSLYAGATDAMVQWWYGHNAVGFFLTAGFLGIMYYFVPKQAERPVYSYRLSIVHFWALITVYIWAGPHHLHYTALPDWAQSLGMVMSLILLAPSWGGMINGMMTLSGAWHKLRSDPILRFLVVSLAFYGMSTFEGPMMAIKTVNALSHYTDWTIGHVHAGALGWVAMVSIGALYHLVPKVFGREQMHSIGLINTHFWLATIGTVLYIASMWVNGIAQGLMWRAINDDGTLTYSFVESLEASHPGFVVRMIGGAIFFAGMLVMAYNTWRTVQAAKPAEYDAAAQI"
  },
  {
    "reference": "MKKLWKKGLVAFLALTLIFQLIPGFASAADSRLKDGGEYQVQVNFYKDNTGKTTKESSEADKYIDHTATIKVENGQPYMYLTITNSTWWQTMAVSKNGTRPEKPAQADVYQDRYEDVQTVSTDAAKDTRVEKFKLSSLDDVIFSYMHIKVDAISYDHWYQVDLTIDPSTFKVISEPAVTTPVTLSDGIYTIPFVAKKANDDSNSSMQNYFNNPAWLKVKNGKKMVAMTVNDNKTVTALKTTLAGTLQDVKVVSEDKDANTRIVEFEVEDLNQPLAAHVNYEAPFNGSVYKGQADFRYVFDTAKATAASSYPGSDETPPVVNPGETNPPVTKPDPGTTNPPVTTPPTTPSKPAVVDPKNLLNNHTYSIDFDVFKDGTTETSMMESYVMKPALIKVENNQPYVYLTLTNSSWIKTFQYKVNGVWKDMEVVSGDINKNTRTVKYPVKDGTANTDVKTHVLIEDMPGFSYDHEYTVQVKLNAATIKDITGKDVTLKEPVKKDILNTGNVASNNNAGPKLAKPDFDDTNSVQKTASKTEKNAKTNDSSSMVWYITLFGASFLYLAYRLKRKRLS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: At low hemin (Hn) and hemoglobin (Hb) concentrations adsorbs Hn/Hb and presumably directs it to membrane transporters (Probable); Acts as an extracellular and cell wall-bound hemophore; scavenges host heme and hemoglobin from the environment and also serves as a cell wall receptor for both (Probable); Binds host hemin (Probable).2. The protein design should prioritize the formation of strand-strand-helix-strand-strand-turn-strand-strand-strand-turn-strand-strand-strand elements.3. Ensure the presence of a signal peptide within the protein.4. The protein should have metal ion binding and be active in cell surface, extracellular region.5. The presence of NEAT domains is critical for the interaction of the designed protein with its intended targets.6. Incorporating a NXZTN sorting signal motif is critical for the successful design of the protein.7. The designed protein's functionality will benefit from Pro residues, Polar residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "ILGGHLDAKGSFPWQAKMVSHHNLTTGATLINEQWLLTTAKNLFLNHSENATAKDIAPTLTLYVGKKQLVEIEKVVLHPNYSQVDIGLIKLKQKVSVNERVMPICLPSKDYAEVGRVGYVSGWGRNANFKFTDHLKYVMLPVADQDQCIRHYEGSTVPEKKTPKSPVGVQPILNEHTFCAGMSKYQEDTCYGDAGSAFAVHDLEEDTWYATGILSFDKSCAVAEYGVYVKVTSIQDWVQKTITL",
    "response#2": "ILGGHLDAKGSFPWQAKMVSHHNLTTGATLINEQWLLTTAKNLFLNHSENATAKDIAPTLTLYVGKKQLVEIEKVVLHPNYSQVDIGLIKLKQKVSVNERVMPICLPSKDYAEVGRVGYVSGWGRNANFKFTDHLKYVMLPVADQDQCIRHYEGSTVPEKKTPKSPVGVQPILNEHTFCAGMSKYQEDTCYGDAGSAFAVHDLEEDTWYATGILSFDKSCAVAEYGVYVKVTSIQDWVQKIREQYPS",
    "response#3": "ILGGHLDAKGSFPWQAKMVSHHNLTTGATLINEQWLLTTAKNLFLNHSENATAKDIAPTLTLYVGKKQLVEIEKVVLHPNYSQVDIGLIKLKQKVSVNERVMPICLPSKDYAEVGRVGYVSGWGRNANFKFTDHLKYVMLPVADQDQCIRHYEGSTVPEKKTPKSPVGVQPILNEHTFCAGMSKYQEDTCYGDAGSAFAVHDLEEDTWYATGILSFDKSCAVAEYGVYVKVTSIQDWVQKTIIPTDQNVVYRGSWYGHIANGTSWSGNASDKEGGNRAEFTVNFADKKITGKLTAENRQAQTFTIEGMIQGNGFEGTAKTAESGFDLDQKNTTRTPKAYITDAKVKGGFYGPKAEELGGWFAYPGDKQTEKATATSSDGNSASSATVVFGAKP"
  },
  {
    "reference": "MNIEQLSIELDKAGLEVVIGLETHIRLNTKSKLFCTCPNEEASDPNTNICSVCTGQMGVLPSLNKEAIRKAIIFGKAVNSSMSNEVICWDRKHYEYPDSPKNFQLTQFQKPIIPDGTVQCYRDNGTTFTVELEQVHIEEDAAKLMHERKQTLVDFNKGGVPLIEVVTKPCMRHLKDASTYAQYLQRIVQNLGISEANLEKGEFKSDVSVSLRKKGSEDLNARTEIKNLNSFKFMVEALEEEVTKQLEYYKEHKDFRPDQTTVLWDEALKQTKVMRKKEFAADYRYALEPDIPFVNIKKEIESITVDASLLPFYIESTLIEGGVRPQDAKFFTSDAARSNLFKILNDEINDTAFVAKTLLNNLKPEDYEKVSKPSALIDMFAAFKAEKITVILLQNAIGSVLENPAFDYQSYFEENSISNDTLSESIAEVLKENAAIADEIKAGNEGKAGILVGKVIAKVGKGASGKVVREQILAQLNAQKVANASNIGKSKGVQTAKQKEEEEESRFKEIIIKDNYRTHLNVDISEEMLGNTAAVAGWVASVRDHGELVFIDLRDSSYEVLQIRLDRANFDNLDDLAKLKPESVIAVSGEIIKRGEDDFNAGIKTGTIEMDAKALDVLNAAKTLPFEIKRAAKSNENVRFKYKFLDHRNAETRTAIVNRHRVIKLIRDILDDQRFIEIETPILTAGTDEGAREFIVPSRKFAGKFYTLPQAPQQFKQMLMVGGFERYFQIARCFRDEDSRGDRQPEFTQLDIEMAFVSMQDIININTE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be designed to have high stability and activity towards ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-   aspartyl-tRNA(Asx).2. The designed protein should contain one or more Aminoacyl-transfer RNA synthetases class-II family profile domains that are essential for its function.3. The designed protein should be involved in aspartyl-tRNA aminoacylation by localizing to cytoplasm and exhibiting aspartate-tRNA(Asn) ligase activity, asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity, transferase activity.4. The ATP, L-aspartate binding site should be located in a region of the protein that is accessible to the ligand.5. The protein must exhibit the following characteristics: Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn); Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).. The designed protein sequence is ",
    "response#1": "MIRTHYCGEVTDMAIGQTVRLKGWVQKRRDLGGLIFIDLRDRTGIVQIVFSPDVSKEALQVAETIRSEYVLDVEGTVVQREEGQVNPNLPTGTIEVHAMHVTILSEAKTPPFLISDKTDVSEDVRLKYRYLDLRRPVHPIDEGGLIKGFCVKGAADLLSKNILQNEYALKLVPAFGGKGMTWMKVAGGRLESNIVQFFSPEEQAGLVARFQAAEGDVLMLIADTDRDLAHEETRLRYRFLDLRREKLHANIMKRGAIIDSLRRRMREGGFFEFQTPILTASSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLTMIAGFDRYFQIAPCFRDEDARADRSPGEFYQLDIEMSFVTQEDVFQAVEPVLRGVFEEFAEGWRVTGTFPRIPYAEAMLKYGVDKPDLRNPLIIVDVTQEFSAEAVTFNAFKSVIRAGGVVRAIPAPGAGAQPRSFFDRLNNWARGEGAPGLGYIVFEEEGGVLSGRGPIAKFVPPEVQAAIAAKAGLKAGDAVFFAAGVEAKAAGLAGKARIRIGDDLGLTDKDQFAFCWITDFPMYEWNEDEKRIDFSHNPFSMPNYDHDGFLGLDPADAETILGIKAFQYDIVCNGIELSSGAIRNHRPDVMEKAFAIAGYGREVLEQKFGGMLNALRMGAPPHGGIAPGVDRIVMLLCHEPNIREVVLFPMNFSGPATDPKGRVAALKVPGGNALTRKQIDDYTKFVSIYGAKGLAYIKVNDKSNLEEGLQSPIVKFLPVEVRAALLERLEAEDGDLIFFGADSAKIVNEA",
    "response#2": "MFTAINKELNDTAFVAKTLLNNLKTEEYAVIENSADLVNIFGALQSGSITQILLQNAIKELLQNASFDYKKYFTENSISDDTLAETIETVIKENQGTADEIKAGNQGKAGILVGKVLQVVGKGANGKVVREMILTQLAGSNGTGSNGTGNNGTNGTNGTNGTNGTNGTNGTNGTNGTNGTNGTNGTNGSSAANAGPTNGTSGAGNTGTGTGAGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGSAGAGAGAGAGAGAGAGAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGAGAGAGVGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGGAGAGAGAGGAGVGAGAGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGAGAGGAGVGAGAGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGVGAGAGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGVGAGAGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGVGAGAGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG",
    "response#3": "MRTHKISQLSEASITEQVTLSGWVASVRDHGELIFIDLRDSSNEIFQVRLSRESFPNLDELVKLKPESVISVTGVVVQRKEDDYNAGLRSGKIELETAVLEILNLSKTLPFEIKRAAKSNEMIRFEYKFLDHRNDEMTDARIIRCIRDFLDERGFVEIETPFLTRSTPEGARDFLVPSRTHQGKFYALPQSPQLFKQLLMVGGMDRYFQIARCFRDEDLRPNRQPEFTQLDLEASFIDEEFIYEMLEELTCRIFAVGAIDLPRPFPRMTYAEAMSRYGSDRPDLRFDMAFEDLTDLFEETGYAIFRRIRDEGGLIKGFCVKGAADLLSKNILQNEYALKLVPAFGGKGMTWMKVAGGRLESNIVQFFSPEEQAGLVARFQAAEGDVLMLIADTDRDLAHKVLAALRLHVAQRLDRIPQDRFRPLWVTDFPLFEWKEGRLSSQHHPFTMPDRTDFDPRDVEGCLRLNSRAYDLVMNGEELGGGSIRIHDMAVQRKIFEALELSPEEAEGKFGFFLNALEFGAPPHAGLALGLDRVIAMILKEPNIREIIAFPKNRSAFCPLTGAPSAVQEEQLRSLGIRLPEKQEKQQES"
  },
  {
    "reference": "MKFIVKLFPEITIKTKPVRQLMTKSLAQNLRNVLRPICAEVKVVNTWDAISVRTPEETAEVVNEAVAQALECVPGISQVQAVKEFPLTDFDAMVAATLEVWGDALAGKTFCVRCKRIGDHDFTSIDVERYVGGGLNQQSDAAGVRLKNPELEIRLEIKHNRFYVIENKRPGLGGYPLGTQESVLTLVSGGFDSTVAAFQMLRRGVKNHFLFFNLGGAAHENGVKEVTYYLWKKYGASHKTHFISVPFEGVVEEILTQVEDRYMGVVLKRMMMRAAERIAEEQNIPALVTGECLAQVSSQTMPNLNLIDEVTDRLVIRPLIVTDKPEIIDQARALGTASFAERMPEYCGVISRKPNAACKRPALLEAEAAFDSAVLEAAIAERRIENVDQLTWTDQESLNETLPTSAAADAVVIDVRHPSEAEGEPLELSGHEVVQIPFYSLNRRFAELDVNKQYLLYCDKGVMSKLHALHLQDAGFRNVGVYRA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be designed to have high stability and activity towards [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 +   S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier   protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-   cysteinyl-[cysteine desulfurase].2. The ATP binding site should be stable and able to withstand changes in environmental conditions.3. A protein should be able to manipulate the activity of enzymes in the Cofactor biosynthesis pathway, either by activating or inhibiting their function.4. The designed protein have THUMP, Rhodanese domains with specific sequence motifs that are crucial for its activity.5. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining ATP binding, tRNA binding.6. The protein must exhibit the following characteristics: This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway; Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate; The sulfur is donated as persulfide by IscS.. The designed protein sequence is ",
    "response#1": "IVKLFPEITIKSKPVRQRLVKQLRQNVQVVLSKETDGAKVQGFWDKIEVRLLPDYAADEPKLIAALQRIPGIANILKVESHELEGIDDRFEDIADICIKTYGDQVSGKSFVVRVKRAGQHSFASTDLERYLGGAFLQNTKASRVELREPDVAIKLEIRDQTLYAIAERYEGLGGFPIGTQEPVLSLISGGFDSTVSSYLTMKRGLKTHFCFFNLGGDAHEIGVKQVSLYLWEKYGRSHRVQFVTVPFEGVVAEILKNVNHSHMGVILKRMMMRAAQEVADQLNIQALVTGESVSQVSSQTITNLNVIDKVCETLVLRPLITMDKQDIIRLSTDIGTEEFAKNMPEYCGVISDRPTTCAKLDRVLEEESKFDFDVLKQAPKLIAALQRIPGIANILKVESHELEGIDDRFEDIADICIKTYGDQVSGKSFVVRVKRAGQHSFASTDLERYLGGAFLQNTKASRVELREPDVAIKLEIRDQTLYAIAEVKPFAEITIKSRPVRRQFILQLGRNIRNVLKDLDPEIRTSGEWDNIDVVSSLTDPALQQQLVERMRHLPGIAHFYQVLDFDLPDSMQDIADRCAEHYAPLLKDKEFAVRCKRVGKHEFTSMDVEREVGSLLRQQCGAAGIRLNRPDVEVRLEIRHRRLLVEFNRQEGLGGYPLGSIEQALVLMSGGFDSTVAAYQLMRRGLLTHFCFFNLGGRAHELGVMEVAQFLWQKFGRSHRTLFISVPFEEVLGDILQNVDNSQMGVILKRQMLRAAEKVADNLHLDVLATGEAISQVSSQTLINLSVIDKATDKLVLRPLVAAHKQDIINTAEAIGTAEFAKHMPEYCGVISVNPTTKAKLGRIVHEEESL",
    "response#2": "IVKLFPEIMIKSDSVRKRFIKILTSNIRNVLTRETEEVAVIRHWDFIEVRAKNPSLAPLVLELLQRTPGIHHILEVEETPFTDMHDIFEQTLKAVREELEGKTFCVRVKRKGKHDFRSLDLERYVGGGLNQHIETASVKLTKPNVTVRIDIDNDKMLLIKARHEGLGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWSRYSTSHKVRFVAINFEGVVGEILEKVDNGQMGVVLKRMMVRAASQVAERFNIQAIVTGEALGQVSSQTLTNLRLIDKAADTLVLRPLITHDKEQIISMAKEIGTDDIAKSMPEFCGVISKNPTVKAVESRIIEEEGHFDFAVLPKLIAALQRIPGIANILKVESHELEGIDDRFEDIADICIKTYGDQVSGKSFVVRVKRAGQHSFASTDLERYLGGAFLQNTKASRVELREPDVAIKLEIRDQTLYAIAEVKPFAEITIKSRPVRRQFILQLGRNIRNVLKDLDPEIRTSGEWDNIDVVSSLTDPALQQQLVERMRHLPGIAHFYQVLDFDLPDSMQDIADRCAEHYAPLLKDKEFAVRCKRVGKHEFTSMDVEREVGSLLRQQCGAAGIRLNRPDVEVRLEIRHRRLLVEFNRQEGLGGYPLGSIEQALVLMSGGFDSTVAAYQLMRRGLLTHFCFFNLGGRAHELGVMEVAQFLWQKFGRSHRTLFISVPFEEVLGDILQNVDNSQMGVILKRQMLRAAEKVADNLHLDVLATGEAISQVSSQTLINLSVIDKATDKLVLRPLVAAHKQDIINTAEAIGTAEFAKHMPEYCGVISVNPTTKAKLGRIVHEEESL",
    "response#3": "IVKPHPEIFVKSESVRKRFTKILECNIRNIVKSRTESVAVYNRRDHIEVTAVSDQYYQEVVEILTHTPGIHHVLEVEQSEFTDLHDIYQQVLAHHRHNIENKTFVVRAKRRGKHDFTSIELERYVGGGLNQAVESAKVKLNKPDITIKVEIADDKLNLIIARHKGLGGFPLGTQEDVLSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQVASYLWNKYGSSAKVRFISVDFEPVVAEILEKVDDGQMGVVLKRMFMRAAGMIAERFGIQALVTGEALGQVSSQTLTNLRHIDGVTDRLILRPLINWDKEDIINLARDIGTEDFAKTMPEYCGVISRKPTVKAVKEKLEAEEDNFDFSILDDVPPSIVEQIRPFAETAIKSRPVRQQQVRQLRRNVRRILARLDDRVQVQGGWDRVDVQLPDDGLTREQVITTLQRVPGISNIQVIREYPLDCFETIADIVVSSFEQRLAGKSFAVRVRRSGKHDFSSQDLEKYLGGALLRRTDARAVNLNHPDEPVRLEVIGDYFHVVDERFEGMGGYPMGAVENAMTLISGGYDSSVATYLNMRRGIRTHFLFFDLGGTAHETGVKQVAWHLWSRYGSSHSVHFITVPFEEVVGEIMRSVNNRYWGVTLKRVMLRAASAIAGETNAQALVTGDSVAQVSSQTLTNINVIDRASEQVVLRPLINMDKGEIIRLAGEIETDEFARNMPEYCGVISSKPVTRAKVHRAEEEEAKMDIRQIAEQTEKDVVKVDTVSVLSANDVILDIRSPEEHDERPFELAGHEVKHLPFYKLSSQFGDLDQSKNYVLYCERGVMSKLQALYLKENGFANVRVF"
  },
  {
    "reference": "MSCSIFKDLPEDHPRRLIPALCRQFYHLGWVTGTGGGMSIKLNNEIYIAPSGVQKERMQPEDLFVQDIDGKDLQMPPEIRELKKSQCTPLFMLAYRHRNAGAVIHTHSQHAVMATLLWPGKTFRCTHLEMIKGVYDDADKRYLQYDEQLVVPIIENTPHERDLADSMYAAMMEHPGCSAVLVRRHGVYVWGQTWEKAKAISECYDYLFSIAVEMKKAGLDPETFEDASKA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The substrate, Zn(2+) binding site should be stable and able to withstand changes in environmental conditions.2. The designed protein should interact with enzymes and substrates within the L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 2/6, Amino-acid biosynthesis, L-methionine biosynthesis via salvage pathway pathway to enhance or regulate its activity.3. For general function, the protein need meet that Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P).4. The design of the protein must focus on the precise arrangement of amino acids in the Proton donor/acceptor to enable effective catalysis of S-methyl-5-thio-D-ribulose 1-phosphate = 5-methylsulfanyl-2,3-   dioxopentyl phosphate + H2O.5. The ideal protein would be one that plays a critical role in L-methionine salvage from S-adenosylmethionine, L-methionine salvage from methylthioadenosine through zinc ion binding, methylthioribulose 1-phosphate dehydratase activity within cytoplasm.6. The presence of Zn(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MTRLIPALCRQFYHLGWVTGTGGGMSIKLNNEIYIAPSGVQKERMQPEDLFVQDIDGKDLQMPPEIRELKKSQCTPLFMLAYRHRNAGAVIHTHSQHAVMATLLWPGKTFRCTHLEMIKGVYDDADKRYLQYDEQLVVPIIENTPHERDLADSMYAGHDGASRLQSWLEAPADGAVIEAGRRSAERGWVPATSGNFSVRVDAERIAVTRSGVDKGRLTPNDVLCQQLSQPLVAGSSAEAELHRRLYADDAEIGAVFHTHAVSATVLAQLHRGERLLTLSGWELQKALAGIRSHETVVEVPVVANDQDVVALANEVAARLAAPVAAGAVRAPGYLIAGHGLYAWGHTAVDAFRHLEALDVLFSDRKVAALE",
    "response#2": "NLIVELCKLFYDNNWVTGTGGGISIREGDTVWLAPSGVQKERMQPTDMFVMDLKSRDYLRRSPTFKPSACTPLFLSAYTLRDAGACIHTHSQAAVMCTLLYDKVFKISNIEQIKAIPQVVESGYLSFFDTLEIPIIENTAHEEDLTDTLQAAIKEYPTCTAVLVRRHGIYVWGETVWKAKVYNEAIDYLLELAGQDDG",
    "response#3": "MNAELIEAGRFIDGRGWVPATSGNFSARLGDGRIAITVSGRHKGRLRPEDIMLVDAAGRSLDGRKPSAETLLHTGIYRRYPEAHAVLHPHSPASTLLSRLVSGAVILEDYELLKALAGIDTHATRIVVPVFPNDQDIPRLALKIEEYLVRHDGVHAYIIAGHGFYTWGKSVADALRHVEALEYLFDLESQIDQTRAKELIEAGRFIDGRGWVPATSGNFSARLGDGRIAITVSGRHKGRLRPEDIMLVDAAGRSLDGRKPSAETLLHTGIYRRYPEAHAVLHPHSPASTLLSRLVSGAVILEDYELLKALAGIDTHATRIVVPVFPNDQDIPRLALKIEEYLVRHDGVHAYIIAGHGFYTWGKSVADALRHVEALEYLFDLEEEVVKPLYQLVTTCHWIGAKGWAPATGGNMSVRQDDTWCWLSESGRDKGSLTTEDFLQVEIATNQAPSGRKPSAETGLHTLVYRLFPEANVVLHVHTVNATVLSRIEKSDTLALQGYEMQKTLSGQHSHLDTVPIAIFDNDQDIDALAARIADYAQTRPLRYGFLLRGHGLTCWGKDIQEARRQLEGLEFLFECL"
  },
  {
    "reference": "MGGIWSKSHTCQWPTVGERMRRAEPAAAGVGAASQDLDNHGALTSSNTDTTNAACAWLRTQEEEEEVGFPVRPQVPLRPMTYKAAVDLSFFLKEKGGLEGLIYSKKRQDILDLWVYNTQGFFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPREVEEANEGENNSLLHPMCLHGMEDQHREVLKWKFDSLLAHRHLARELHPEFYKDC",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike.2. The designed protein should contain at least one Dileucine internalization motif; necessary for CD4 internalization motif to achieve its intended function.3. The ideal protein would be one that plays a critical role in suppression by virus of host autophagy through GTP binding within host cell plasma membrane. The designed protein sequence is ",
    "response#1": "MEPVDPSLEPWKHPGSQPKTACT",
    "response#2": "MGGKWSKSSIVGWPRIRERIRRTEPAAEGVGAVSRDLEKHGAITSSNTAATNADCAWVEAQEEEGEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPREVEEANEGENNCLLHPMSQHGIEDEDREVLKWKFDSSLARRHLARELHPEYYKDC",
    "response#3": "MGSKWSKSSIVGWPEIRERIRRAEPAAEGVGAVSQDLDKHGAITSTNMNNADCAWLRAQEEEEEEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLDGLIYSKRRQEILDLWVYHTQGFFPDWQNYTPGPGTRYPLTFGWCYKLVPVDPSEVEEYSKGENNSLLHPMSLHGMEDPEREVLEWKFDSSLARRHLAREKHPEFYKDC"
  },
  {
    "reference": "MEPRMESCLAQVLQKDVGKRLQVGQELIDYFSDKQKSADLEHDQTMLDKLVDGLATSWVNSSNYKVVLLGMDILSALVTRLQDRFKAQIGTVLPSLIDRLGDAKDSVREQDQTLLLKIMDQAANPQYVWDRMLGGFKHKNFRTREGICLCLIATLNASGAQTLTLSKIVPHICNLLGDPNSQVRDAAINSLVEIYRHVGERVRADLSKKGLPQSRLNVIFTKFDEVQKSGNMIQSANDKNFDDEDSVDGNRPSSASSTSSKAPPSSRRNVGMGTTRRLGSSTLGSKSSAAKEGAGAVDEEDFIKAFDDVPVVQIYSSRDLEESINKIREILSDDKHDWEQRVNALKKIRSLLLAGAAEYDNFFQHLRLLDGAFKLSAKDLRSQVVREACITLGHLSSVLGNKFDHGAEAIMPTIFNLIPNSAKIMATSGVVAVRLIIRHTHIPRLIPVITSNCTSKSVAVRRRCFEFLDLLLQEWQTHSLERHISVLAETIKKGIHDADSEARIEARKCYWGFHSHFSREAEHLYHTLESSYQKALQSHLKNSDSIVSLPQSDRSSSSSQESLNRPLSAKRSPTGSTTSRASTVSTKSVSTTGSLQRSRSDIDVNAAASAKSKVSSSSGTTPFSSAAALPPGSYASLGRIRTRRQSSGSATNVASTPDNRGRSRAKVVSQSQRSRSANPAGAGSRSSSPGKLLGSGYGGLTGGSSRGPPVTPSSEKRSKIPRSQGCSRETSPNRIGLARSSRIPRPSMSQGCSRDTSRESSRDTSPAR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2; Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.; Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN).2. The ideal protein would be one that plays a critical role in establishment of epithelial cell polarity, regulation of focal adhesion assembly, positive regulation of basement membrane assembly involved in embryonic body morphogenesis, cell division through microtubule plus-end binding, kinetochore binding within cell cortex, cytoplasmic microtubule, kinetochore. The designed protein sequence is ",
    "response#1": "LQERFDRLVQLQCKSFPTSRCVTLQVSRDQTQLQDIVQALFQEPYNPARLCEFLLDNCIEPVIMRPVMADYSEEADGGTYVRLSLSYATKEPSSAHVRPNYKQVLENLRLLLHTLAGINCSVSRDQHVFGIIGDHVKDKMLKLLVDECLIPAVPESTEEYQTSTLCEDVAQLEQLLVDSFIINPEQDRALGQFVEKYETYYRNRMYRRVLETAREIC",
    "response#2": "MEPRMESCLAQVLQKDVGKRLQVGQELIDYFSDRQKSADLEHDQTLLDKLVDGLATSWVNSSNYKVVLLGMDILSALVTRLQDRFKAQIGTVLPSLIDRLGDAKDSVREQDQTLLLKIMDQAANPQYVWDRMLGGFKHKNFRTREGICLCLIATLNASGAQTLTLSKIVPHICNLLGDPNSQVRDAAINSLVEIYRHVGERVRADLSKKGLPQSRLNVIFTKFDEVQKSGNC",
    "response#3": "FLFPRCMISKSAQDFVKLMDRILRQPTDKLGDQEADPIAGVISIMLHTYINEVPKVHRKLLESIPQQAVLFHNNCMFFTHWVAQHANKGIESLAALAKTLQATGQQHFRVQVDYQSSILG"
  },
  {
    "reference": "MTKPNTRFVFVTGGVVSSLGKGIAAASLAALFEARGLSVTMQKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFSHATMSKRSNFTTGRIYESVIRRERRGDYLGATVQVIPHITNAIKDAILSLTADSPDIAIVEIGGTVGDIESQPFLEAIRQLRFDLGRKRTAYMHLTLVPYIASAGEIKTKPTQHSVQKLREIGIQPDVLLCRTETPIPQSQKDKIALFCNVDKEAVIEAPDVDTIYAVPLTLCEGGLGTTMLEHFGLPNDNPDLSLWVNICHRIRNARHEVHIAMVGKYVELADAYKSVNESLFHGGMAHDCKVKIDYIDAESLELDGTDCLRGAHGILVPGGFGERGTEGKIAAIRFARENSVPYLGICLGMQMAVVEFARHVAGIEHATSSEFNEQSEHPVIALMTEWEQEGEKIHRTSDDDLGGTMRLGGYPCKVIEGTSTYAAYGKTEIRERHRHRYEFNNTYRAQLEQAGLIIAGTLPDNSLVEIIEVRDHPWFVACQFHPEFLSRPYAPHPLFAGFIGAARKFSGS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that is capable of catalyzing reactions within the CTP from UDP: step 2/2, Pyrimidine metabolism pathway with high efficiency and specificity.2. The designed protein must possess glutaminase activity, metal ion binding, CTP synthase activity, ATP binding to support glutamine metabolic process, 'de novo' CTP biosynthetic process under certain conditions.3. The designed protein is the optimization of the Nucleophile; for glutamine hydrolysis to achieve high catalytic efficiency for H2O + L-glutamine = L-glutamate + NH4(+).4. The designed protein should contain one or more Glutamine amidotransferase, CTP synthase N-terminal domains that are essential for its function.5. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.6. The UTP, L-glutamine, Mg(2+), CTP, ATP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "KYIFVTGGVVSSLGKGIAAASIGCLLESRGLRVTLQKFDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTYAPLTQSNNVTSGRIYETIISRERRGDYLGKTVQVIPHVTDEIKANIEKVSGGVDVVLVEIGGTVGDIESLPFLEAIRQLRHKKGRENCFFVHVTLVPWIAAAGELKTKPTQHSVKELRTIGIQPDMLICRCEKPLAGDIKDKIALFCDVSADAVIEACDAKSVYEVPLGFASQNVDDIILRQFKLEGREPDLTEWRDLVERVKGAKREVRIGIVGKYMDLHDAYKSVYEALFHAGLAGQVAVELVKIDSSKLEEAASADSILGPEAAGGGVDGVLVPGGFGQRGINGMVKAAEWARVNKVPYLGICLGMQIMVIDWARGVLGWEDGDSSEFNPDTKHPVVSLLEEQVDVKNYGGTMRLGKSESAAEPGTRIFAAYGKAQIFERHRHRYEFSNKYRTEMAGSGLRISALTPDGSLVEAVEWPDHPWGVGVQFHPEFKSKPTAANPLFRDFIGAAVDYS",
    "response#2": "MAKYIFVTGGVTSSLGKGIIAASLAKLLQARGFKVTIQKFDPYINVDPGTLNPYEHGECYVTDDGAETDLDLGHYERFLNKPTSQANNVTTGRVYKSVIEKERRGDYLGKTVQVVPHITDEIKRRVQLLGQSGEHEIVITELGGTVGDIEALPYLEALRQMRWTLGSENCLVIHLTLIPYLRAAKELKTKPTQHSVKELQNAGVQPDVLVCRTEYPLNEGLRKKLALFCNVQYDAVIEAIDAKTIYHVPLHMQEEGLDELVLRKFKLSDAYLSVAEALRAGGFKHYTKVEIRWVPSDDCETATGAAAELDDVHGVLIPGGFGIRGIEGKVGAINYARKRGIPVLGLCLGLQCIVIEAARSVGLTEASSAEFDPDTPDPVISTMADQESIVAGEADLGGTMRLGSYPAVLQKNSIVAQAYGSTEVTERHRHRYEVNNAYRDKIAESGLQFCGTSPDGHLVEFVEYPKDVHPFLVGTQAHPELKSRPTRPHPLFAAFIEAIASAKNRKLPDAYLSVAEAIRAGGFGANVRAKVKWVASDDCETPESAAEALKDVDGVVIPGGFGIRGIEGKIGAITYAREHKLPLLGICLGLQCIVIEAARNAGLTDASSTEFDPDAATPVISTMEEQKEAVSGKADLGGTMRLGSYPATLKPDSLVAKLYGREDVTERHRHRYEVNNAYRDAITEGSQLQFSGTSPDGQLVEFVEYPTDVHPYFVATQAHPEYKSRPTRSHPLFQGLIDKIKTRG",
    "response#3": "KYIFVTGGVLSGLGKGITASSIGRLLKSRGVKVTSIKIDPYLNVDAGTMNPFEHGEVYVLDDGGEVDLDLGNYERFLDIPITSAHNITTGKVYKSVIEKERTGEYLGKTVQIIPHITNEIKAQIVNVAEATGAEVCIVELGGTVGDIESMPFLEAVRQMNTELGKGRSCLFVHTTLVPVMGTVGEQKTKPTQHSVKELRAIGIQPDIIVARCKDPLEESIKRKIALFCDVPMEAVVSAADARSIYDVPMTLEAQGITEFILQRLSLPDAYLSVAEAIRAGGFGANVRAKVKWVASDDCETPESAAEALKDVDGVVIPGGFGIRGIEGKIGAITYAREHKLPLLGICLGLQCIVIEAARNAGLTDASSTEFDPDAATPVISTMEEQKEAVSGKADLGGTMRLGSYPATLKPDSLVAKLYGREDVTERHRHRYEVNNAYRDAITEGSQLQFSGTSPDGQLVEFVEYPTDVHPYFVATQAHPEYKSRPTRSHPLFQGLIDSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASFVDSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASFV"
  },
  {
    "reference": "MSDSLRIIFAGTPDFAARHLDALLSSGHEVVGVFTQPDRPAGRGNKLTPSPVKVLAEQHNIPIFQPKSLRPAENQAMVQALDADVMVVVAYGLILPQPVLSMPRLGCINVHGSLLPLWRGAAPIQRALWAGDSETGVTIMQMDVGLDTGAMLHKISCPILPQDTSATLYDKLAELGPRGLLETLELLADGSAVAEAQNDALATYAEKLSKEEARLNWQLSAEQLERCIRAFNPWPVSYFTVDEQPVKVWKAEVITTAHSTLPGTILQADKQGIQVATAVGILNIQELQPAGKKVMSAQDLLNSRREWFVPGNTLD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. We are looking for a protein with broad substrate specificity for (6S)-10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) =   (6S)-5,6,7,8-tetrahydrofolate + H(+) + N-formyl-L-methionyl-   tRNA(fMet). The designed protein sequence is ",
    "response#1": "MGLRIIFAGTPDFAARHLGALLSSQHQIVGVFTQPDRPAGRGNKLTPSPVKVLAEQHDIPIFQPKSLRPEENQHLVADLNADIMVVVAYGLILPASVLAMPRLGCINVHGSLLPRWRGAAPIQRSLWAGDAKTGVTIMQMDVGLDTGDMLHKIECDIQPEDTSATLYDKLAQLGPQGLLVTLQQLAEGSAQPEVQDEAQVTYAEKLSK",
    "response#2": "MRIIFMGTPDFAVPSLKAIAAMDGDFEVQLVVTGKDKPRRSKRSEPEPTPVKKAARELGIPVMEVDDVKDERFVDTVARYRPDVLVVAAFRILPPAVYEQARLGAFNLHASILPRYRGAAPVNWAIINGERESGVTTFFLRKSVDTGNMILQEKTPVYPEENAGELAARLAEIGAGAVVKTLELIRDGRVEPLLQNDTLATRAPKKLSKEEAKLDWSLSAVQLERCIRAFNPWPVSYFVVDEQPIKVWQAQVLATVDNAAPGTIIHADKHGIQVATADGVLNITQLQPAGKKAMSAADLLNSL",
    "response#3": "GLRIIFAGTPDFAARHLGALLSSQHQIVGVFTQPDRPAGRGNKLTPSPVKVLAEQHDIPIFQPKSLRPEENQHLVADLNADIMVVVAYGLILPASVLAMPRLGCINVHGSLLPRWRGAAPIQRSLWAGDAKTGVTIMQMDVGLDTGDMLHKIECDIQPEDTSATLYDKLAQLGPQGLLVTLQQLAEGSAQPEVQDEAQVTYAEKTNLVKLSKEEAKLDWSLSAVQLERCIRAFNPWPVSYFVVDEQPIKVWQAQVLATVDNAAPGTIIHADKHGIQVATADGVLNITQLQPAGKKAMSAADLLNSATALLGR"
  },
  {
    "reference": "MWLNDLIPSELAGDAGRTRITGFAIDHRKVAPGTIFGAFRGSRVNGEDYIDQAIASGAAAIVAAPDAKVVGAPHIADTEPRRAFAALAARFFAPFPDTVVAVTGTNGKTSTVELTRQLWGMAGYHAASIGTLGVTTAHDQVSTGLTTPDVVTFLSNMAGLRREGVTHAAFEASSHGLSQYRTEGLPVRAAAFTNLSRDHLDYHGTMESYFDAKMRLFTEVVDDGGAAVIWADDLMSDKVIAIAKARGLQLHTVGEMGEAIRLLDRQPTALGQKLTIAVDGKPRTITLPLIGAYQAANALVSAALVIATGGGVDRTLSDLQRVAPVRGRLERAVITSSGAPVYVDYAHTPDGLDAAIQALRPHARDRLIVIFGAGGDRDTGKRPLMGDVAVRLGDLVIVTDDNPRSEDPAAIRRDVLAGAIGAIEIGSRRDAIAAGVAEAGPGDIVLIAGKGHEQGQIVGDRILPFDDVTVARECAV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have a high specificity for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding, with minimal off-target effects.2. The protein design should take into account the physiological conditions of the peptidoglycan biosynthesis pathway.3. The protein must bind to Mg(2+) in order to perform its enzymatic function.4. The designed protein have Mur ligase central, Mur ligase C-terminal domains with specific sequence motifs that are crucial for its activity.5. The designed protein should be involved in peptidoglycan biosynthetic process by localizing to cytoplasm and exhibiting ATP binding, magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity. The designed protein sequence is ",
    "response#1": "MTLDSRVAAAGDLFVAVKGHQADGRRYIPQAIAQGVAAVVADADSAAGHGTLTMTHGVPVIAIHNLQQQLSALAGRFYGEPSRELRLIGITGTKGKTTTTYLVKSILENAGREAGLIGTIENRIGDRVIPTERTTPDAVALQKLFAEMVEEGCTHVVMEVSSHALEQRRVDGCEFDVAVFTNLTQDHLDYHKTMENYFAAKRRLFTEYLAPGKKHAAAVINADDPYGRRLADEIPASCVTYAIDHPADITAHNLSVGADGVQFKLDGKLGHVDIALRLPGHFNVYNALAATGAAIADGVPLEAVVKGIEGALTVSGRMQLVAQRENGATVFVDYAHKPGAVIAALGSLRPHVMGRIVCVIGAGGDRDRGKRSLMGQAARDFADVVIVTDDNPRTEDPAAIRAAVMEGAGPEATEIPDRAEAILRGVDALLPGDALLICGKGHETYQIIGDKKHHFDDREVAREAIARRLARA",
    "response#2": "VTGTNGKTTTTQLLAQWSQALGETSAVMGTIGNGLLGQVVPAENTTGSAVEVQQALYQLAEKGATFAAMEVSSHGLIQHRVAALPFAAALFTNLSRDHLDYHGDMAGYEAAKWLLFSTHQAGQKIINADDEVGLRWLQQLPDAVAVSVDGKVPAGWGGQSLSAHAVQYHDNGVRVEFSSSWGNGVLDARLIGAFNVSNVLMALATLLSLGYPFDDVLVTGTSGKTTVAHLLEAGLTAAGRSSGLLGTVRTRIGEESLPSAFTTPEAPDLQAVFALMAERGVTDVAMEVSSHALALGRVAGTRFAVGAFTNLSQDHLDFHRDMADYFAAKARLFDGRAHRHVVCIDDEWGRRLAAATPGAVTVSTTGPAADWRATGVAGDPDGSQRFVAVTPDGGELPVHLRLPGAFNVANALVALAMTLDSRVAAAGDLFVAVKGHQADGRRYIPQAIAQGVAAVVADADSAAGHGTLTMTHGVPVIAIHNLQQQLSALAGRFEGRERRFQTIGTSETIYGDREWARLALDAAS",
    "response#3": "VTGTNGKTTTTFLTKHICEAAGLRCGLIGTVRYEIGERVLPAIRTTPESLDLVELLAQIRDAGCRAAAMEVSSHALAQDRTRGLEWNVAVFTNLTQDHLDFHGTMMSYFDAKAKLFTQLGAQQKKRKPMAVVNIDDRYGEQLLDKIDKNVAVITYGRGARADFRASNPRVEFSGTSYQLEAGGKSYLVRVPLIGRFNVLNSVAALAAITGTNGKTTTSHIIEKIFKDAGQKTGLIGTMYTKIGEQTFDVKNTTPESLTLQKTFKKMVDEQVDTAVMEVSSHALAYGRVHGTDYNVAVFTNLTQDHLDYHKTMDEYKKAKGLLFSQLGNTFNHEAPKFAVLNADDPASKDFAMLTAAHLLTYGIDNYADLQAMNISMTASGTTFDLVSPFGTMKVTIQLIGKFSIYNVLASIGAALELGIPFEKILDTLPKFHGVAGRLELVPAKRQFQVFVDYAHTPDALRNVLRTLRELEPHRLIAVFGCGGDRDRQKRPMMGDIVDQYADYAIITSDNPRTEDPNAILAEIEKGFRSSRYEKIVDRTEAIGRAVALAQPRDMVLIAGKGHEDYQEIKGVKYPFDDRETAREALRERGL"
  },
  {
    "reference": "MEPVDPRLEPWNHPGSQPKTACNNCYCKRCCYHCLYCFTKKGLGISYGRKKRSQRRRTPQSSKSHQDLIPEQPLSQQQGDQTGQKKQKEALESKTEADPCD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be involved in negative regulation of peptidyl-threonine phosphorylation, suppression by virus of host translation initiation, positive regulation of transcription elongation by RNA polymerase II by localizing to host cell nucleolus, host cell cytoplasm and exhibiting protein domain specific binding, trans-activation response element binding.2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.3. The protein must exhibit the following characteristics: In the vascular system, Tat binds to ITGAV/ITGB3 and ITGA5/ITGB1 integrins dimers at the surface of endothelial cells and competes with bFGF for heparin-binding sites, leading to an excess of soluble bFGF.; Circulating Tat also acts as a chemokine-like and/or growth factor-like molecule that binds to specific receptors on the surface of the cells, affecting many cellular pathways; Neurons are rarely infected, but they internalize Tat via their LDLR; Modulates the expression of many cellular genes involved in cell survival, proliferation or in coding for cytokines or cytokine receptors. The designed protein sequence is ",
    "response#1": "MGSRHSCCAWLLLLAVLRAAGSVXGAXGXTDGDTXPXXDNXTLXXQARXGXHXXQX",
    "response#2": "MGGKWSKSSIVGWPAIRERMRRAEPAAEGVGAASQDLDKYGALTSSNTASTNADCAWLQAQEEEEEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLEGLVYSKKRQEILDLWVYNTQGFFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPREVEEANEGENNCLLHPMSQHGMEDEDREVLMWKFDSSLARRHIARELHPEYYKDC",
    "response#3": "MEPVDPSLEPWKHPGSQPRTACTSCYCKKCCFHCQVCFITKGLGISYGRKKRRQRRRTPQGSQTHQASLSKQPTSQPRGDPTGPKESKKKVERETETDPSD"
  },
  {
    "reference": "MTRFIFITGGVVSSLGKGLASAALGALLQARGFTVRLRKLDPYLNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTGVASRQSDNITTGRIYQNVISRERRGEYLGATIQVIPHVTDAIKTFISSDLTDEDFVLCEIGGTVGDIESLPFLESIRQLGNELGRERAMYIHLTLLPYVPTAGELKTKPTQHSVKELLSVGIQPDILLCRTDRDLPETERQKIALFCNVRREAVIPALDVESIYQVPISYHAEGLDEQVCGHFGLEVPKPDLTAWRAIVERATKPEGEVRIAVVGKYTGLLDAYKSLAEALAHGGIANNVRVKMHWIDSEIFEREDSVAHLNGVNGILVPGGFGERGAEGKIAAARFARERNVPYFGICFGMQMAVVEAARNLVGLEEAGSTEFAPTPEPVVGLLTEWERAGTMEKRSADSDLGGTMRLGAYDCVMSPDSRVRQIYRNDHVSERHRHRYEVNMAYEDRLAGKGVIFAGKSPDGALPEIMEIPDHLWFIGVQFHPELKSRPFEPHPLFESFIGAAVEQARLV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.2. The designed protein should contain one or more CTP synthase N-terminal domains that are essential for its function.3. The designed protein should have a binding site that is specific to the target UTP, CTP ligand.4. The Nucleophile; for glutamine hydrolysis, active site, Nucleophile active site of the designed protein must be optimized for substrate binding and specificity.5. The protein design must be focused on enhancing the efficiency of 'de novo' CTP biosynthetic process. The designed protein sequence is ",
    "response#1": "VTIAVVGKYTSLLDSYKSLGEALQHGGIANKVKVHLKWIDSQIFENEGAIHELDDCHGILVPGGFGERGVTGKIAAVRFAREHKVPYFGICFGMQMAVIEAARNLAGIRNASSSEFGACDEPVVGLMTEWLHGNKLVQRKASDDLGGTMRLGAYGCHLVPGTKVAEIYGADVISERHRHRYEVNIDYRDRLENAGMVFSGMSPDGLLPEIVERPDHPWFIGVQFHPELKSKPFDPHPLFTSFVEKALA",
    "response#2": "RFIFITGGVVSSLGKGLASAAMGALLQARGYSVRLRKLDPYLNVDPGTMSPFEHGEVFVTDDGAETDLDLGHYERFTGVSARQTDSISSGRIYSNVLEKERRGDYLGKTIQVIPHVTNEIKDFISIGEDEVDFMLCEIGGTVGDIEGLPFFEAIRQFAQDKPRGQCIFMHLTLLPYIKASGELKTKPTQHSVKELRSIGLAPDILVCRGEGAIPQKEREKLALFCNVRPDAVIAAPDLASIYEAPLAYHREGLDQAVLDAFQIDAGPADLSEWDAVVEAERNPAAELQDAYKSIYESFTHAGAENDARVEILRVDAEELEEKGAEALIGSVDGLLVPGGFGDRGIEGKIQAVQYARTKGIPFMGICLGMQVAVIEYARHICGMDDANSTEFDKATSHPVICLQEEQKGIENMGATMRLGAYKALITPGTLAHKLYGKDNVNERHRHRYEFNPAYREDLEKAGLVISAINDEHGLVEVVELPTHPFFIACQYHPEFQSAPNRAHPLFSGLLDFLNRLREDAYLSIREALKHAGVDAGCIVDIKWIEAEELENLENPEELLGDVGGILVPGGFGIRGAEGKIKAIKFAREHKIPFLGICFGFQLAVVEFARHRLGWEGASSTEITDTPYPVIEILPEQLDVEDLGGTMRLGDSEITVLKGTRAYAIYGKEQIVERHRHRYEVNPEYIEAIEEGGMRFSGKSGNRMEILEIADHPFFIGTQFHPEFSSRPGKPAPVFRAFVD",
    "response#3": "RFIFITGGVVSSLGKGLASAAMGALLQARGYSVRLRKLDPYLNVDPGTMSPFEHGEVFVTDDGAETDLDLGHYERFTGVSARQTDSISSGRIYSNVLEKERRGDYLGKTIQVIPHVTNEIKDFISIGEDEVDFMLCEIGGTVGDIEGLPFFEAIRQFAQDKPRGQCIFMHLTLLPYIKASGELKTKPTQHSVKELRSIGLAPDILVCRGEGAIPQKEREKLALFCNVRPDAVIAAPDLASIYEAPLAYHREGLDQAVLDAFQLDCGEADLSEWEQVVDAMEHPQVELHDAYLSVSESLFHAAAANDAVCDIKWVDSEELTQENIDDVLGDCEGILVPGGFGDRGIEGMILAARYAREKDVPYLGICLGMQIAVIEFARNVVGWADANSAEFTSSSLHPVIDLMPEQQGITAKGGTMRLGQYPCALDKESKAYELYGEETVYERHRHRYELNNDFREELCEKGLRLAGLSPDGRLVEVVENPANRWFVAAQFHPEFKSRPNKPQPLFYGFVKSRLAQ"
  },
  {
    "reference": "DCIPGGENCDVFRPYRCCSGYCILLLCA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have calcium channel regulator activity, sodium channel regulator activity and be active in extracellular region. The designed protein sequence is ",
    "response#1": "DGEVNNLQLALARIQVLGHRASRAIASYISSHCRFRWPKVETQLGMKPPLTSSEAKNHIATDAVSAAVGNLTKPALSSPKENHGDFITDPNVWVSVPIAEGESDLDELEEDMEQASQSSWQEEDPKGQQEQLPQVQKCENHQAARSPASMMSSEDLAPYLGESWKRKDSPQVPAEGVDDTSSSEGSTVDCPDPEEILRKIPELADDLDEPDDCFTEGCTRRCPCCNVNTSKSPWATGWQVRKTCYS",
    "response#2": "HPFFQLFILISVLIDCVFMSLTNLPKWRPVLENTLLGIYTFEILVKLFARGVWAGSFSFLGDPWNWLDFSVTVFEVIIRYSPLDFIPTLQTARTLRILKIIPLNQGLKSLVGVLIHCLKQLIGVIILTLFFLSIFSLIGMGLFMGNLKHKCFRWPQENENETLHNRTGNPYYIRETENFYYLEGERYALLCGNRTDAGQCPEGYVCVKAGINPDQGFTNFDSFGWALFALFRLMAQDYPEVLYHQILYASGKVYMIFFVVVSFLFSFYMASLFLGILAMAYEEEKQR",
    "response#3": "DGEVNNLQVALARIQVFGHRTKQALCSFFSRSCPFPQPKAEPELVVKLPLSSSKAENHIAANTARGSSGGLQAPRGPRDEHSDFIANPTVWVSVPIAEGESDLDDLEDDGGEDAQSFQQEVIPKGQQEQLQQVERCGDHLTPRSPGTGTSSEDLAPSLGETWKDESVPQVPAEGVDDTSSSEGSTVDCLDPEEILRKIPELADDLEEPDDCFTEGCIRHCPCCKLDTTKSPWDVGWQVRKTCYR"
  },
  {
    "reference": "MKYDIVVVGSINMDVIVQCPQYPEYGDTVFCNSIAMTPGGKGANQAATVARLGKKTALIGAVGKDSAGSSLLQNLQSKGVDTTHMLEIENQGTGTFVAMVDSTGENTMVGTKGANDFITKEDIDGIFNQIEAKILLVQMETSKESIIAAMKAAKERNMYVILDPAPADGIFEEAFQYADLVVPNKQETERITGIEVTDQETALLAAKKIREIGVSDVIVKMAENGSLVYQNDVPTLIDAIKVTAVDTVGAGDCYAGAIASAYLDTNDLIEAAKFASVAAGIKVSRTGGQDAIPTLEEVEEYRKTVSV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The K(+) binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The designed protein sequence is ",
    "response#1": "VPILAEYFREKGLENLCVVSPDLGGVTRARNLAERIGASIAIIDKRRPEPNVSEIMNVIGELKGKTVIMIDDIIDTAGTITQGAQALIERGAKEVYACCTHAVLSGSALERLENSVIRELVVTNG",
    "response#2": "ASPVLVSYFRDLELPNLTVVSPDAGGVERARFFAKKLEVPLAIVDKRRTDINVTEVMNVIGDVRGRTCLILDDIIDTAGTLVKTADALLDQGAAKVYACATHAVLSGPAVERIATSRLEELIVTNR",
    "response#3": "SPVLTDDIERQRYKNLIVVSPDVGGVVRARAVAKQLNKDLAIIDKRRPTANEAQIMHIXGDVEGRSCLIVDDMVDTAGTLCKAADALKENGAKKVVAYCTHPVLSGTAIENIEGSNLDALVVTDE"
  },
  {
    "reference": "MSNIPTELKYASSHEWIRKEEDGSYTVGITEHAQELLGDMVFVELPEVGDTVTAGDDCAVAESVKAASDIYAPISGEVIAVNEALEDSPELVNSDAYGEGWFFRVMPSDESEVDALLDAEGYQAVIDEE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must be engineered to have a cooperative binding mechanism with (R)-lipoate to ensure proper regulation.2. The protein should be optimized for stability and expression in cytoplasm, glycine cleavage complex where it will participate in protein lipoylation. The designed protein sequence is ",
    "response#1": "WESGTSTLMILGDRCTRACSFCDVATGRPGDLDLEEPERAAEAIERMGLRHAVITSVNRDDLDDGGSGIFAETIEAVHRRVPECDVEVLIPDFKGSRQDLQTVMDAGPAVLNHNTETVLRLQRDVRTAANYGRSLALLARAKWMNPTGSVKSGLIVGMGETKDEILGALADLRAVGVDIVTIGQYLRPSAKHRPIHRYVHPDEFAEYKDYGERLGLRAG",
    "response#2": "MILGDRCTRACSFCDVATGRPGDLDLEEPERAAEAIERMGLRHAVITSVNRDDLDDGGSGIFAETIEAVHRRVPECDVEVLIPDFKGSRQDLQTVMDAGPAVLNHNTETVLRLQRDVRTAANYGRSLALLARAKWMNPTGSVKSGLIVGMGETKDEILGALA",
    "response#3": "TVTVGVTDIGQARAGKIINIRIKPPGKYVKKGKPVASLESGKWAGPVPADIEGEVVERNEKLFDKPDLINEDPYGEGWIAKLE"
  },
  {
    "reference": "MHSAQKKNIRHFDLPSLQQYFQEIGEKPFRAKQVYEWLWLKHAPTFEAMTNLSKSLRQQLEANFSLPAVTVDTLQKSEDGTMKSRFRLHDGYFVEGVLIPTDTRQTACVSSQVGCSLSCKFCATGTMGRKRNLHFDEIYDEVALLNQQALESSGKKLTNIVYMGMGEPLLNYNNVLKSIDRITAADGLAMSPKRITVSTAGIAKMIKQLGDDKVRFNLALSLHAANDRKRNEIMPINETNDLKSLVEALNYFYKQTESQISFEYILFRNFNDSQQDADELIKIYRQVPCDLVNIIEYNPIDGGRFAKPDEDKTQAFMDYLGKHRVNSRLRRSRGKDIDAACGQLANKTA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be able to modulate rRNA base methylation in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "KTACVSSQIGCSLSCKFCATGYIERKRNLSFDEIYDEVVLINQQSMEVYDKKLTNIVFMGMGEPLLNYRNVLKAIEKITSPDALAMSPRRITVSTAGVAKQIRQLGDDQVKFKLALSLHAANDKKRHEIMPINDTNNIQSLIEALNYFYRQTKNEITFEYILFNNFNDSLEDADELIKIYRQVPADLVNLIEYNPIEFAKFTKPSEEKVDVFMNYLETA",
    "response#2": "TTQVGCRVGCTFCASTLGGLKRDLTAGEIIAQVVQAQQRLDNSNERISSIVIMGIGEPFENYDHMMKFLKTMVHKQGLNIGQRHITVSTSGIVPNIYKFADEKTQINLAISIHAPNDALRSSLMPVNRRFPLRDLLKACKYYIQTTGRRITFEYALIGGKNDRPEHV",
    "response#3": "SSQVGCQLGCTFCATGLSGFKRDLRTSEIVAQVLYMEQKIGKEVNNIVFMGMGEPFLNNEAVFKSIEILHEKKGRNLGIRHFTISTAGIPEGIKKLANSGLDVKLSVSLHAADDEKRSELMPINRRFPLKELFNILDYYQKETGNRITFEYILIDGINDSLEDARKR"
  },
  {
    "reference": "MVDIVICLGIEGTAEKTGVGIVDSDGNILAMQGCQLYPEKGGIHPRIAAEHHAEWIPKLIPKAIEEAAINYSDLDLISFSQGPGLGPALRIVATSARSLALSLNKPIIGVNHCIGHVEVGKLDTGAVNPVSLYVSGGNSQVIAYESGRYRIFGETLDIAIGNCLDQFGRETGLGHPGGPVIEKLAKKGNYIDLPYVVKGMDFSFSGLLSAALRQHQKGADIEDVCFSLQETAFAMLVEVTERALSHTQKDEVMLCGGVSANSRLREMLKTMSEEHGAKFYMPEMKLCGDNGVMIAWLGLLMCKEFGPMDLADTGIIQRFRTDEVDIPWIDNTKNYLKLSDDLIAKGAESNILKSSYMGENAVIKARIPKNYRIPEIDNKIRKSRCKLEAKLLSDAKRAGVRTPILYDVDLAEKSILMEAIDGVMLKEVIDEDLSFKVGREIAKLHSVDIIHGDITSSNIMLRDDELIFIDFGLGRYSDLDEDKAVDLLVLKKSLQSVDYEKAVSYFRSVLEGYGNSKIIDKISDIEHRGRYTH",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that can perform N(6)-L-threonylcarbamoyladenine synthase activity, metalloendopeptidase activity, zinc ion binding in cytoplasm to support protein phosphorylation, tRNA threonylcarbamoyladenosine modification.2. The designed protein should have a high specificity for Fe cation binding, with minimal off-target effects.3. The presence of Protein kinase domains is critical for the interaction of the designed protein with its intended targets.4. For general function, the protein need meet that Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A375. The designed protein is the optimization of the Proton acceptor; for kinase activity to achieve high catalytic efficiency for adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP +   H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA. The designed protein sequence is ",
    "response#1": "MICLGIEGTAEKTGVGIVDGDGNILAMTGNQLFPEEGGIHPRMAAEHHAEWIPKLIPQAIEESGLSYEDIDMISFSQGPGLGPGLRIVATSARSLALSLKKPIIGVNHCIAHVEVGKLDTGAVNPVSLYVSGGNSQVIAHESGRYRIFGETLDIAIGNCLDHFGRETGLGHPGGPVIEKLSKQGSYIDLPYVVKGMDFSFSGLLSAALREAKKGTPIEDICFSLQETAFSMLVEVTERALSHTQKDEVMLCGGVAANSRLREMLNTMSEEHGAKFYMPEMKLCGDNGVMIAWLGLLMFREFGSMDLSQTGIIQKFRTDEVDIPKIRRARTKEEAKLLSDAKCAGVKTPILYDINLEEKSILMEEIKGKMVKDVMDEDLAFKIGVEIAKLHAADIIHGDITSSNMMLQDGKLVFIDFLG",
    "response#2": "MICLGIEGTAEKTGVGIVDGDGNILAMTGNQLFPEEGGIHPRMAAEHHAEWIPKLIPQAIEESGLSYEDIDMISFSQGPGLGPGLRIVATSARSLALSLKKPIIGVNHCIAHVEVGKLDTGAVNPVSLYVSGGNSQVIAHESGRYRIFGETLDIAIGNCLDHFGRETGLGHPGGPVIEKLSKQGSYIDLPYVVKGMDFSFSGLLSAALREAKKGTPIEDICFSLQETAFSMLVEVTERALSHTQKDEVMLCGGVAANSRLREMLNTMSEEHGAKFYMPEMKLCGDNGVMIAWLGLLMFREFGSMDLSQTGIIQKFRTDEVDIPESKYMDKDEILNLPEELRAKGAESDIYEATWLGREAIVKNRVSKSYRIDEIDNKIRKLRTKSEAKILSDVKKTGVRAPVLYDVDFEDKSIVMEKINGSLVKDIMHEIGEDKRRELAIAIGENIKAFHDGDIIHGDLTGSNMILIDDDLDNISDNLAIFDFGLGKYSDLLEDKAADLLVLKKSFQSIDYDIATETFDWILEAYDSNNQSKMANKIAEIEGRGRYTL",
    "response#3": "MICLGIEGTAEKTGIGIVDSDGNILATCGDQLYPEVGGIHPREAANFHAEHIVPLIKDALTEAQLTLNDIDLVSFSKGPGLGPALRTVATAARSLSQNINVPLIGVNHCIGHVEIGKLTTGAKDPVTLYTSGGNTQIISYETGRYRIIGETLDIAIGNCLDQFSRDVGLGHPGGPIVEKHAQNTEETVELPYIVKGMDLSFSGILTSAINKYKKGTDLDVICNSFQETCFAMLCEVTERALSYTGKDEVLLCGGVAANKKLRQMLKQMCDEHYVEFYMPPMKYCGDNGSMIARLGLLSYNENLTGIENSYINPKYRTDHVDVTNNIAIKKVRISKNYRIPEIDDKIRKKRTKHESKLLSHLKRLGIRTPVLYDIDLKNSAIIMEKIEGKTIDELLNNQNVEKDKLIAIFENIGKTIAVLHDGDVIHGDITTSNIMIDTYNNLVLLDLGLGKYDNKIRKLRTKSEAKILSDVKKTGVRAPVLYDVDFEDKSIVMEKINGSLVKDIMHEIGEDKRRELAIAIGENIKAFHDGDIIHGDLTGSNMILIDDSDEWLQRLITERTRAEARIIAMARRAGVPTPVIRDITRDTIIMECIEGSLLKHVLDERTAREAGRVVGRLHGAGIVHGDLTTSNIILRDETCVLIDFGLEIIHGDLTTENILLDDKEVIIKHRLKKNYRNELLDNKLRLERTKQEAKLLSDSKDLCVRTPYIYSIDLENKSIIEEKINATKLHDVIVDAEDKGSLRKLFNAIGEDVSKLHDGEIIHGDLTTENILIDDDKPVFIDFGLGKY"
  },
  {
    "reference": "MINFFGGKAPTAAQPASSTSTAVNGARFASVSTSSSASASAAGQPPLKKTKIAPSPVTVVTTVKKKVAPPTSVEPRDPIAALSGYNSKNALPEEKRRRIIEERLAAQRQKSIESSLEAQLRAPTKSLSKSQSASSTSRRSAASFSKPKTKKPRKKVVDSDSDHDEFGDYGKSSRKPSTAPSTPRASKRTSVDPHPTSDSYQKVGRDGVQPNYTVPRDIRASTSPTNTTAIPIDTQSRPSKPISSADIVTANIKNYGPYFNGLGDAPRAVLEYPGTDASEEFLLLVPKDADEYDPLMELLATVRAIVTHYLTPEQRKAFGSLDSLEVSNNAGMILPSATHGASASTSAVCNMVAALTDSNGINGARAKSADLLDGQGSKAERRNHLVGAGTYKDTGSMTPEPHANGISQTAPVSPTSQTSGISASAGGSSVAKTVLVNNALLRLDSPAPTELSVASDHSSTPAASCGTDPDSILRSFTKARNRRDGPLFMRTLARFNSALAALRDTGALAANIADLGSCTGVPEGIWRLIQDQVYARVVGPRVEELGRYQAFSDNVYGELLPRFMSEIAQLTLLGPEKVFVDLGSGVGNLLIQTSLQTGAEAYGCEMMRIPASLASQQVVEAQLRWAAWGLRGGSAIEAWQGDFGDHGGVRDVLKRADVVLVNNYAFLPKTNENLSLLFLDLPDGAKVVSLKPFVPPDFRLTQRTLSSPLAILRVTERLYTSGCVSWADGGGKYYIQEVDRSLVREFLQNAGAATRAKRGKVSEGDDTV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein have enhanced L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-methionine = 3 H(+)   + N(6),N(6),N(6)-trimethyl-L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-   homocysteine.2. A protein that contributes to histone H3-K79 methylation, DNA damage checkpoint signaling, regulation of transcription regulatory region DNA binding by functioning in nucleus with histone H3K79 methyltransferase activity, histone H3K79 trimethyltransferase activity.3. The protein must exhibit the following characteristics: Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3; This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks; Nucleosomes are preferred as substrate compared to free histone..4. The protein should have a high affinity for S-adenosyl-L-methionine.5. The designed protein must have at least one DOT1 domain.6. Target a Polar residues, Basic and acidic residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "MDARVHRSKRGCGRVLKRSRASSPEVVAEVGTGRPGDPFVLPLRSSPNGSGCHHCPEHSCDCLYVSKELERCYLSLQVSRQVTCKGRRELCAKSVLPPFVARLLRVANVTADDTFYDLGCGNGSVLFHVALATGASCVGVEINEHNAKVAKEAWTHLRPVFEKRRGRKLDVSIVCGDFCKVLKQDNYFSSSCVVWIANLLMPRFVNHYLSERLRSLPIGSRVLCMEDLYPHSRSVAAARDPDAFEKFEMVDYRWQEDSVEWSPASGPFYLYIKRDSGFEILPCNRYSSEQNGAKIVATKEWKRNDKIELLVGCIAELSEIEENMLLRHGENDFSVMYSTRKNCAQLWLGPAAFINHDCRPNCKFVSTGRDTACVKALRDIEPGEEISCYYGDGFFGENNEFCECYTCERRGTGAFKSVRLEVFKTANRGWGLRSWDAIRAGSFICIYVGEAKDKSKVQQTMANDDYTFDTTNVYNPFKWNYEPGLADEDACEEMSEESEIPLPLIISAKNVGNVARFMNHSCSPNVFWQPVSYENNSQLFVHVAFFAISHIPPMTELTYDYDSGFEILPCNRYSSEQNGAKIVATKEWKRNDKIELLVGCIAELSEIEENMLLRHGENDFSVMYSTRKNCAQLWLGPAAFINHDCRPNCKFVSTGRDTACVKALRDIEPGEEISCYYGDGFFGENNEFCECYTCERRGTGAFKSGEGLYAKCDVDQDTVMAFYNGVRLKQDEVENRDWSQNSNTISLTDDIAIDVPEEYVSTDNYCASLGHKVNHSFDPNCRYDIYQHPRFGFIKCVRTIRGVSEGDELTVH",
    "response#2": "MDARVHRSKRGCGRVLKRSRASSPEVVAEVGTGRPGDPFVLPLRSSPNGSGCHHCPEHSCDCLYVSKELERCYLSLQVSRQVTCKGRRELCAKSVLPPFVARLLRVANVTADDTFYDLGCGNGSVLFHVALATGASCVGVEINEHNAKVAKEAWTHLRPVFEKRRGRKLDVSIVCGDFCKVLKQDNYFSSSCVVWIANLLMPRFVNHYLSERLRSLPIGSRVLCMEDLYPHSRSVAAARDPDAFEKFEMVDYRWQEDSVEWSPASGPFYLYIKRMDARVHRSKRGCGRVLKRSRASSPEVVAEVGTGRPGDPFVLPLRSSPNGSGCHHCPEHSCDCLYVSKELERCYLSLQVSRQVTCKGRRELCAKSVLPPFVARLLRVANVTADDTFYDLGCGNGSVLFHVALATGASCVGVEINEHNAKVAKEAWTHLRPVFEKRRGRKLDVSIVCGDFCKVLKQDNYFSSSCVVWIANLLMPRFVNHYLSERLRSLPIGSRVLCMEDLYPHSRSVAAARDPDAFEKFEMVDYRWQEDSVEWSPASGPFYLYIKRLPCNRYSSEQNGAKIVATKEWKRNDKIELLVGCIAELSEIEENMLLRHGENDFSVMYSTRKNCAQLWLGPAAFINHDCRPNCKFVSTGRDTACVKALRDIEPGEEISCYYGDGFFGGAKIVATKEWKRNDKIELLVGCIAELSEIEENMLLRHGENDFSVMYSTRKNCAQLWLGPAAFINHDCRPNCKFVSTGRDTACVKALRDIEPGEEISCYY",
    "response#3": "MDARVHRSKRGCGRVLKRSRASSPEVVAEVGTGRPGDPFVLPLRSSPNGSGCHHCPEHSCDCLYVSKELERCYLSLQVSRQVTCKGRRELCAKSVLPPFVARLLRVANVTADDTFYDLGCGNGSVLFHVALATGASCVGVEINEHNAKVAKEAWTHLRPVFEKRRGRKLDVSIVCGDFCKVLKQDNYFSSSCVVWIANLLMPRFVNHYLSERLRSLPIGSRVLCMEDLYPHSRSVAAARDPDAFEKFEMVDYRWQEDSVEWSPASGPFYLYIKRGEGLYAKCDVDQDTVMAFYNGVRLKQDEVENRDWSQNSNTISLTDDIAIDVPEEYVSTDNYCASLGHKVNHSFDPNCRYDIYQHPRFGFIKCVRTIRGVSEGDELTVHVRLEVFKTANRGWGLRSWDAIRAGSFICIYVGEAKDKSKVQQTMANDDYTFDTTNVYNPFKWNYEPGLADEDACEEMSEESEIPLPLIISAKNVGNVARFMNHSCSPNVFWQPVSYENNSQLFVHVAFFAISHIPPMTELTYDYGVSRPSAA"
  },
  {
    "reference": "MKVVVASSNSGKLAEFERLLAPLGFDCVPQSALGVEDADETGLTFVENALIKARHAARVTGLPALADDSGLCVDALGGAPGLYSARYAGPQADPTANITKLLDALRDRADSERGAYFHAVIVLLRHAEDPRPLIAEGSWHGHILDAARGAGGFGYDPVFLDPERGVSAAELDPSIKARVSHRGRAVDALRAQLADWRAT",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP2. The protein design must be able to perform nucleotide binding, metal ion binding, dITP diphosphatase activity, XTP diphosphatase activity, ribonucleoside triphosphate phosphatase activity in order to contribute to purine nucleoside triphosphate catabolic process, nucleotide metabolic process.3. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate, Mg(2+) binding.4. The protein with the reaction dITP + H2O = dIMP + diphosphate + H(+), the Proton acceptor must be optimized to ensure efficient catalysis.5. The protein must bind to Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MPKVVLATHNPHKLAELKAILEGVQCEVVGLDDVPEFDEAPETGATFEENAIAKAQAASKATGLPALADDSGLCVDALNGEPGIYSARFAGEHATDQQNLDKLLSELRTIPTEKRSARFVCAIALVTPTGEEHVVEGRMPGTLLTEPRGENGFGYDPVFIPDGYDLTTAELSPDEKNAISHRGRAMRALIPALRALL",
    "response#2": "MKIIFATGNQHKASEIADILGNRFEIISLSDIGCTEDIPETADTLEGNALLKANYVFEKYNINCFADDTGLEVDALNGEPGIYSARYAGENCSYEDNVNKLLGKLKNKSNRKARFRTVISLIIDGKEKIFEGIVNGTIIDEKRGTDGFGYDPIFKPDGYDITFAEMSMDEKNSISHRGKAVEKLVEFLLKN",
    "response#3": "MTITTPKQATLDSASPAFKPSDSSLPAFSQGMPPAQRQEIIRNSFKTVPRHLLASQPIARLAYDDGPLPLSKYQRGEPIATASIPQAQGMCCGPVSIAVMLQALAHTVTPDSKVLELGSGTGFNAALMAHLVGPHGKVTTVDINASVLQAAQQNLAKAGYTNVTVLNADGTKGWPEHAPYDKILVTAGAPNVPEALIEQLAIGGRLVIPVGPTAIGQTLIKVTKVENDFITKSLHSVAFVPLTDAE"
  },
  {
    "reference": "MNMDWALFLTFLAACGAPATTGALLKPDEWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLAAMRVAQLEGSGQALAFYAAQLAFNTLWTPVFFGMKRMATALAVVMVMWLFVAATMWAFFQLDTWAGVLFVPYLIWATAATGLNFEAMRLNWNRPEARA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should contain a Helical; Name=3, Helical; Name=5, Helical; Name=4, Helical; Name=2, Helical; Name=1 membrane-spanning region.2. The designed protein should be involved in tetrapyrrole metabolic process by localizing to membrane, plasma membrane and exhibiting lipid binding, tetrapyrrole binding.3. The protein must exhibit the following characteristics: May play a role in the transmembrane transport of tetrapyrroles and similar compounds, and thereby contribute to the regulation of tetrapyrrole biosynthesis; Binds protoporphyrin IX, hemin, and coproporphyrin III, but does not bind delta-aminolevulinic acid; Binds tetrapyrroles and promotes the photooxidative degradation of protoporphyrin IX; Can bind bilirubin, curcumin, gossypol, retinoic acid, cholesterol and the benzodiazepine receptor agonist PK-11195 (in vitro).4. helix-helix-helix-helix-helix-helix-helix-turn-turn is the desired arrangement for the engineered protein. The designed protein sequence is ",
    "response#1": "MGLLVMAYGTPYKEEDIERYYTHIRRGRKPEPEMLQDLKDRYEAIGGISPLAQITEQQAHNLEQHLNEIQDEITFKAYIGLKHIEPFIEDAVAEMHKDGITEAVSIVLAPHFSTFSVQSYNKRAKEEAEKLGGLTITSVESWYDHHDTALIVSAHSLPEKIKEFGDPNFYWVDRVKETYASMPEDERENAMLIVSAHSLPEKIKEFGDPYPDQLHESAKLIAEGAGVSEYAVGWQSEGNTPDPWLGPDVQDLTRDLFEQKGYQAFVYVPVGFVADHLEVLYDNDYECKVVTDDIGASYYRPEMPYWVDRVKETYASMPEDERENAMLIVSAHSLPEKIKEFGDPYPDQLHESAKLIAEGAGVSEYAVGWQSEGNTPDPWLGPDVQDLTRDLFEQKGYQAFVYVPVGFVADHLEVLYDNDYECKVVTDDIGASYYRPEMPGFRRRYWVDRVKETYASMPEDERENAMLIVSAHSLPEKIKEFGDPYPDQLHESAKLIAEGAGVSEYAVGWQSEGNTPDPWLGPDVQDLTRDLFEQKGYQAFVYVPVGFVADHLEVLYDNDYECKVVTDDIGASYYRPEMPGFRRRYFPEYWVDRVKETYASMPEDERENAMLIVSAHSLPEKIKEFGDPYPDQLHESAKLIAEGAGVSEYAVGWQSEGNTPDPWLGPDVQDLTRDLFEQKGYQAFVYVPVGFVADHLEVLYDNDYECKVVTDDIGASYYRPEMPGFRRRYLPVTAS",
    "response#2": "MGLLVMAYGTPYKEEDIERYYTHIRRGRKPEPEMLQDLKDRYEAIGGISPLAQITEQQAHNLEQHLNEIQDEITFKAYIGLKHIEPFIEDAVAEMHKDGITEAVSIVLAPHFSTFSVQSYNKRAKEEAEKLGGLTITSVESWYDLGPKVFKLDQLPGTIGKKAAKGASVKFSESSTQAVIKATYLQVFGRDVYEGQRLKVQEIKLENGEISVRDFVRALAKSDLFRKLYWTPFYVCKAIEYIHRRLLGRPTYGRQENNKYFDIASKKGLYAVVDAILDSLEYTETFGEDTVPYERYLTPAGVALRQLRVGTIREDVANVEKQLRSEILYWVDRVKETYASMPEDERENAMLIVSAHSLPEKIKEFGDPYPDQLHESAKLIAEGAGVSEYAVGWQSEGNTPDPWLGPDVQDLTRDLFEQKGYQAFVYVPVGFVADHLEVLYDNDYECKVVTDDIGASYYRPEMPYWVDRVKETYASMPEDERENAMLIVSAHSLPEKIKEFGDPYPDQLHESAKLIAEGAGVSEYAVGWQSEGNTPDPWLGPDVQDLTRDLFEQKGYQAFVYVPVGFVADHLEVLYDNDYECKVVTDDIGASYYRPEMPLFAPDFLRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGERRGAPQDAEQMGISLAEELLKG",
    "response#3": "MGLLVMAYGTPYKEEDIERYYTHIRRGRKPEPEMLQDLKDRYEAIGGISPLAQITEQQAHNLEQHLNEIQDEITFKAYIGLKHIEPFIEDAVAEMHKDGITEAVSIVLAPHFSTFSVQSYNKRAKEEAEKLGGLTITSVESWYDKAQLIVSAHSLPEKIKEFGDPDFYENYWVDRVKETYASMPEDERENAMLIVSAHSLPEKIKEFGDPYPDQLHESAKLIAEGAGVSEYAVGWQSEGNTPDPWLGPDVQDLTRDLFEQKGYQAFVYVPVGFVADHLEVLYDNDYECKVVTDDIGASYYRPEMPYWVDRVKETYASMPEDERENAMLIVSAHSLPEKIKEFGDPYPDQLHESAKLIAEGAGVSEYAVGWQSEGNTPDPWLGPDVQDLTRDLFEQKGYQAFVYVPVGFVADHLEVLYDNDYECKVVTDDIGASYYRPEMPSWLEPNYWVDRVKETYASMPEDERENAMLIVSAHSLPEKIKEFGDPYPDQLHESAKLIAEGAGVSEYAVGWQSEGNTPDPWLGPDVQDLTRDLFEQKGYQAFVYVPVGFVADHLEVLYDNDYECKVVTDDIGASYYRPEMPTWKEPDAQYWVDRVKETYASMPEDERENAMLIVSAHSLPEKIKEFGDPYPDQLHESAKLIAEGAGVSEYAVGWQSEGNTPDPWLGPDVQDLTRDLFEQKGYQAFVYVPVGFVADHLEVLYDNDYECKVVTDDIGASYYRPEMPSWREPEARYDYWVDRVKETYASMPEDERENAMLIVSAHSLPEKIKEFGDPYPDQLHESAKLIAEGAGVSEYAVGWQSEGNTPDPWLGPDVQDLTRDLFEQKGYQAFVYVPVGFVADHLEVLYDNDYECKVVTDDIGASYYRPEMP"
  },
  {
    "reference": "MPHLMVTSLPCQSPLYTWTGPQGSSGQICPSPCTSASEPRGLTPARSRRPYLLAWDGREAGPPLHLVQVAQVLADGARDDALVVLVTLRRGQSARKWHRAAGARSSSPGLLQALPWRGLPPPPLHVALLGGQAEVLISSASRSSAGSPRPAPSGRVRAMELWPWLTAALLLLLLLVQLNRAAKFYTKITLYCVLCFVVSAVASVVCLLRHGGRTVENMSIISWFVRSFKYAYGLRFEIKGRGKLEVDHPCVIISNHQSILDMMGLMEILPPRCIQIAKRELLFLGPLGLIMYLGGVVFINRQRSSTAMTVMADVGEHLVREDLKVWIYPEGTRNDNGDLLPFKKGAFHLAIQAQVPIIPVVYSSFSSFYNSRTKLFTSGTVKVEVLDAIPTRGQLGHLRRREAEGHQAWRVSGCGKERPERRAGERWGFLFIPPSGRPAGQPQGQDGSVGHSRAQLRARCS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be optimized for activity in Phospholipid metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The protein must exhibit the following characteristics: Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone..3. The protein should have a Helical domain that is long enough to span the entire membrane.4. A protein that contributes to positive regulation of cytokine production, phosphatidic acid biosynthetic process by functioning in membrane, endoplasmic reticulum with 1-acylglycerol-3-phosphate O-acyltransferase activity.5. The protein should be designed to catalyze the following reaction a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-   sn-glycero-3-phosphate + CoA.6. The protein contains novel Phospholipid/glycerol acyltransferase domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MQVRGSEHLNLQEPFVIVCNHQASLDLLGMVEVIPERCVPIAKKELKYMGTVGWACWLSGIIFIDRFRREVAIDVISKTAETVRRENLRVWIFPEGTRNQSKSMLPFKRGAFHLAVQAQVPILPIVIRFEIKGRQKLEEDRSCVIISNHQSILDMMGLMEVLPDRCVQIAKRELLFLGPVGLIMYLGGVLFINRQHSRTAMTVITTVGEHMVREKLKVWIYPEGTRNDNGDLLPFKKGAFYLAVQAQVPIIPVVYSSFSSFYNKREKRFTSGTCTIKVLPTIHTSGLTNDNVPFLMQRLRDTMFEALSELS",
    "response#2": "MQVRGSEHLNLQEPFVIVCNHQASLDLLGMVEVIPERCVPIAKKELKYMGTVGWACWLSGIIFIDRFRREVAIDVISKTAETVRRENLRVWIFPEGTRNQSKSMLPFKRGAFHLAVQAQVPILPIVIRFEVKGLENFKVEGPAIIVSNHQSILDMMGLMEVLPDNCVQVGKKELMYTGTVGLIIYLGGVIFINRKSTSSAKMVMSEVAKTMVADNVKVWVYPEGTRNCTGDLLPFKKGAFHLAVQAQVPVIPVVYSSFSSFYNKVEKRFRPGTIRVEVLDAIPTQGLSAADVPKLTLQVAGAMRSALAKMS",
    "response#3": "GMQLVVKGKENLRTKKPFVLVLNHQTSLDILVMLEILPHRCIPIGKKEILYMGTFGLALWLAGTVFIDRSDREGSINTLTELAQSLHKDNLRVLIFPEGTRNHGGSMLPFKRGAFQLAVKAQVPIIPLVLGLRFEIKGRQKLEEDRSCVIISNHQSILDMMGLMEVLPDRCVQIAKRELLFLGPVGLIMYLGGVLFINRQHSRTAMTVITTVGEHMVREKLKVWIYPEGTRNDNGDLLPFKKGAFYLAVQAQVPIIPVVFSSYNNFYCRKEKRFTSGTCTIRVLPRVDTKGMSPGDVPGLTDQVRKTMLEALSELSTT"
  },
  {
    "reference": "MCAAEVDAHIARRFRLQRRLGKGAYGIVWKAVDRSTGEVVAIKKIFDAFRDQTDAQRTFREIVLLQEFGGHPNIIRLLDVIRAENDRDIYLVFESMDTDLHAVIEKGTLLKDIHKRYIFYQLLRATQFLHSGHVVHRDQKPSNVLLDANCLVKLCDFGLARSLSSVPEGPEGQPLTEYVATRWYRAPEVLLSSHWYTPGVDMWSLGCILGEMLRGRPLFPGTSTLHQLQLILEAVPPPSEEDLLALGSNYSSLVLHHLGSRPRQLLDALLPPDTPPQALDLLRRLLVFAPDKRLSAAQALQHPYVQRFHCPGQEWTRDAAFQLPVPAGVQLSATEYRNRLYQMLLERRGSGGLGGAAPEPGAAPQEPGRRHRSSGGRAPERGACCRPRRVPSGLRGPCPDSGSVCPAEAPRAATSKSRQRPAPLLQLPPPGRRARPPAPAAEAPLAPAGVVSRAAGAGGASHCALGAAPAAPSQGRPAPSLTSQAVAQAANRALIRSDQTRVSGLRAAGGLRGSRGPGPPAPPLLSPARSRPLPGAQAPPRDAPEPRPGRRMLGSTSALQGAQGAARAALGGYSQAYGTVCRSALDCLPLLPRPAPEPPRGNSRTCSVLLPNPVPAPLQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The ATP binding site should be stable and able to withstand changes in environmental conditions.2. Target a Pro residues compositional bias in the protein's composition for improved properties.3. The designed protein should have ATP binding, MAP kinase activity, protein serine kinase activity, chromatin binding within ciliary basal body, autophagosome, centriole, cell-cell junction to regulate regulation of COPII vesicle coating, positive regulation of telomere capping, protein autophosphorylation, regulation of autophagy, cellular response to DNA damage stimulus. The designed protein sequence is ",
    "response#1": "WKVLRKIGGGGFGEIYDALDMLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNYVVMQLQGRNLADLRRSQSRGTFTISTTLRLGRQILESIESIHSVGFLHRDIKPSNFAMGRFPSTCRKCYMLDFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRNREMGRHDDLWSLFYMLVEFVVGQLPWRKIKDKEQVGSIKERYDHRLMLKHLPPEFSIFLDHISSLDYFTKPDYQLLTSVFDNSR",
    "response#2": "KVLRKIGGGGFGEIYDALDMLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNYVVMQLQGRNLADLRRSQSRGTFTISTTLRLGRQILESIESIHSVGFLHRDIKPSNFAMGRFPSTCRKCYMLDFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRNREMGRHDDLWSLFYMLVEFVVGQLPWRKIKDKEQVGSIKEL",
    "response#3": "PWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPTTIFIDEIDSICSRRGTSDEHEASRRVKSELLVQMDGVGGALENDDPSKMVMVLAATNFPWDIDEALRRRLEKRIYIPLPTVEERERLIRLHFEKN"
  },
  {
    "reference": "MENRWQVMIVWQVDRMRIKTWISLVKHHKYVSGKARKWIYRHHYENPHPRVSSEVHIPLGDARLVITTYWGLHTGERDWHLGQGVSIEWRKERYSTQIDPDLADRLIHLYYFDCFSESAIRNAILGRIVRGRCDYPSGHNRVGSLQYLALTALIAPKKTKPPLPSIRKLTEDRWNKPQKTKDHRESHTMNGH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that contributes to viral life cycle by functioning in virion component, plasma membrane, host cell plasma membrane, host cell cytoplasm, cytoplasm with RNA binding.2. For general function, the protein need meet that Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions; Finally, Vif also contributes to the G2 cell cycle arrest observed in HIV infected cells.; Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism; Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G; Recruits an active E3 ubiquitin ligase complex composed of elongin BC, CUL5, and RBX2 to induce polyubiquitination of APOBEC3G and APOBEC3F; In turn, they are directed to the 26S proteasome for degradation; Seems to play a role in viral morphology by affecting the stability of the viral nucleoprotein core; May interact directly with APOBEC3G mRNA in order to inhibit its translation3. The protein design should emphasize Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPEIRERIRQTPPAATGVGAVSQDLDKHGAVTSSNINHPSCVWLEAQEEEEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLDGLIYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGIRYPLTFGWCYKLVPVDPKEVEEANEGENNCLLHPMSQHGMEDEDREVLVWKFDSSLARRHIAREQHPEFYKDC",
    "response#2": "MRVKETQMNWPNLWKWGTLILGLVIICSASDNWXNXKXNXTXXXTXXXVXSXAXRXREXEXEXTXRXAXGMXXVXVPVXRXVXEEXGXAXKXGXGXEXXXIXRXXXRXXTXPXXXHXLXQXXXIXXXIPXXXT",
    "response#3": "MGGKWSKSSIVGWPAVRERXRSXPAAEGVGAVSQDLDKHGAITSSNINHPSCVWLEAQEDEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQKRQDILDLWVYHTQGYFPDWQNYTPGPGXRYPLTFGWCFKLVPVDPDEVEKATEGENNSLLHPICQHGMDDEEREVLKWKFDSQLAHRHMARELHPEFYKDC"
  },
  {
    "reference": "MNFQAGGGQSPQQQQSLAAPGGGGAAAQQLVCGGQFGGAGPGAGGGGPSQQLAGGPPQQFALSNSAAIRAEIQRFESVHPNIYAIYDLIERIEDLALQNQIREHVISIEDSFVNSQEWTLSRSVPELKVGIVGNLSSGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPELQFAAWVDAVVFVFSLEDEISFQTVYNYFLRLCSFRNASEVPMVLVGTQDAISAANPRVIDDSRARKLSTDLKRCTYYETCATYGLNVERVFQDVAQKVVALRKKQQLAIGPCKSLPNSPSHSAVSAASIPAVHINQATNGGSSAFSDYSSSVPSTPSISQRELRIETIAASSTPTPIRKQSKRRSNIFTSRKGADLDREKKAAECRVDSIGSGRAIPIKQGILLKRSGKSLNKEWKKKYVTLCDNGLLTYHPSLHDYMQNIHGKEIDLLRTTVKVPGKRLPRATPTTAPGTSPRANGLAMERSNTQLGGATGAPHSASSTSLHSERPHSSAWAGSRPGPEGLHQRSCSVSSTDQWSEAAALPASMQHPASGPAESLSSSPKLEPPPSPHSNRKKHRGKKSTGTPRPDGPSSAAEEAEESFEFVVVSLTGQTWHFEASTAEERELWVQSVQAQILASLQGCRSAKDKTRLGNQNTALAVQAVRTVRGNSLCIDCEAPNPDWASLNLGALMCIECSGIHRHLGAHLSRVRSLDLDDWPPELLAVMTAMGNALANSVWEGALDGYSKPGPEACREEKERWIRAKY",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for GTP binding.2. For general function, the protein need meet that GTPase which may be involved in the degradation of expanded polyglutamine proteins through the ubiquitin-proteasome pathway.3. The designed protein must have at least one PH, Arf-GAP domain.4. The designed protein should have GTPase activity, GTPase activator activity within cytoplasm to regulate activation of GTPase activity. The designed protein sequence is ",
    "response#1": "VKISVVTKRERSKVPYIVRQCIEEVEKRGIEEVGIYRISGVATDIQALKAVFDANNKDILLMLSDMDINAIAGTLKLYFRELPEPLLTDRLYPAFMEGIALSDPAAKENCMMHLLRSLPDPS",
    "response#2": "MKSGFVALAGKPNVGKSSIVNAIVGKKILIVSDKPQTTRNRINVIHTTDDFQIIFVDTPGIHKPLYRLGEYMVKAAVSALKGVDLILTVVDAKEGVGKPERFVFDYVNQSKTKTIGVINKIDLVDAKKVEQIYNEIKNSLENCVGIVKTSAVRGEGIKELLDLL",
    "response#3": "MKSGFVALAGKPNVGKSSIVNAIVGKKILIVSDKPQTTRNRINVIHTTDDFQIIFVDTPGIHKPLYRLGEYMVKAAVSALKGVDLILTVVDAKEGVGKPERFVFDYVNQSKTKTIGVINKIDLVDAKKVEQIYNEIKNSLENCVGIVKTSAVRGEGIKELLDLWEALPTVAVVGGQSSGKSSVLESIVGRDFLPRGSGIVTRRPLVLQLHKTDDGTEEYAEFLHLPKKQFTDFALVRREIQDETDRITGKNKQISPVPIHLSIYSPNVVNLTLIDLPGLTKVAVEGQPETIAEDIESMVRTYVDKPNCIILAISPANQDIATSDAIKLAKDVDPTGERTFGVLTKLDLMDKGTNALEVLEGRSYRLQHPWVGIVNRSQADINKNVDMMLARRKEREYFDTSPDYGHLASKMGSEYLAKLLSKHLESVIRTRIKSGFVALAGKPNVGKSSIVNAIVGKKILIVSDKPQTTRNRINVIHTTDDFQIIFVDTPGIHKPLYRLGEYMVKAAVSALKGVDLILTVVDAKEGVGKPERFVFDYVNQSKTKTIGVINKIDLVDAKKVEQIYNEIKNSLENCVGIVKTSAVRGEGIKELLDLIVENLEK"
  },
  {
    "reference": "MAPVGSKRGVLERLNAGEVVIGDGGFVFALEKRGYVKAGPWTPEAAAEHPEAVRQLHREFLRAGSNVMQTFTFYASDDKLENRGNKLSFTGQQINEAACDLAREVANEGDALVAGGVSQTPSYLSCKSEEEVKKTFKKQLDVFIKKNVDLLIAEYFEHVEEAEWAVQVLKATGKPVAATLCIGPDGDMHGVIPGECAVRLVKAGADIVGVNCHFDPLTCVKTVAMMKAAVEKAGLKAHYMTQPLAYHTPDCSCQGFIDLPEFPFALEPRILTRWEMQQYAREAYKAGIRYIGGCCGFEPYHIRAVAEELSAERGFLPEASQKHGLWGSGLEMHTKPWVRARARRDYWEKLKPASGRPLCPSMSTPDGWGVTRGHAALMQQKEATTAEQLRPLFQQADAKH",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must have at least one Hcy-binding domain.2. The ideal protein would be one that plays a critical role in type B pancreatic cell development, amino-acid betaine catabolic process, digestive system development, methylation, L-methionine salvage through betaine-homocysteine S-methyltransferase activity, zinc ion binding within cytosol.3. The presence of Zn(2+) is crucial for the protein to function optimally under specific conditions.4. We need a protein that can perform glycine betaine + L-homocysteine = L-methionine + N,N-   dimethylglycine with high efficiency.5. For general function, the protein need meet that This reaction is also required for the irreversible oxidation of choline .; Converts betaine and homocysteine to dimethylglycine and methionine, respectively; Involved in the regulation of homocysteine metabolism6. A protein that is capable of catalyzing reactions within the L-methionine biosynthesis via de novo pathway, Amino-acid biosynthesis, L-methionine from L-homocysteine (BhmT route): step 1/1 pathway with high efficiency and specificity.7. The protein should have a high affinity for Zn(2+). The designed protein sequence is ",
    "response#1": "MVLDGAMGTEIQKFKLKDNDYRGEEFKDFPHELGGNNDLLSLTQPHIIREIHCKYLEAGADFIETNTFNGNIFSQADYKMEHLVKRINIESARLAKSACEEYTKKDPSRPRFVCGAVGPTNKTASISPSVERPEARNVLFDELVSGYLEQVEALVEGGIDVILVETVFDSLNCKAALFAIEEFFKTYSPRLPVFVSGTIVDKSGRTLSGQTGEAFYTSVASANLMVFGLNCALGAQEMRPFLQNISKCSECYVSCYPNAGLPNTFGGYDETPEMMAEQIKEFAESGLLNIVGGCCGTSPDHIRAFCNEVEEWRNKPVSERIAHALVKGITTYIIEDTEEARNTLPSSLSVIEGPLMGGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLIPFMEEEKRLKHGAYGIMANQSERIAHALVKGITTYIIEDTEEARNTLPSSLSVIEGPLMGGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLGCMQVKPVSERIAHALVKGITTYIIEDTEEARNTLPSSLSVIEGPLMGGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLIPFMEEEK",
    "response#2": "MVLDGAMGTEIQKFKLKDNDYRGEEFKDFPHELGGNNDLLSLTQPHIIREIHCKYLEAGADFIETNTFNGNIFSQADYKMEHLVKRINIESARLAKSACEEYTKKDPSRPRFVCGAVGPTNKTASISPSVERPEARNVLFDELVSGYLEQVEALVEGGIDVILVETVFDSLNCKAALFAIEEFFKTYSPRLPVFVSGTIVDKSGRTLSGQTGEAFYTSVASANLMVFGLNCALGAQEMRPFLQNISKCSECYVSCYPNAGLPNTFGGYDETPEMMAEQIKEFAESGLLNIVGGCCGTSPDHIRAFCNIYWGLDTEDHFGIIRKILSERIMVLDGAMGTEIQKFKLKDNDYRGEEFKDFPHELGGNNDLLSLTQPHIIREIHCKYLEAGADFIETNTFNGNIFSQADYKMEHLVKRINIESARLAKSACEEYTKKDPSRPRFVCGAVGPTNKTASISPSVERPEARNVLFDELVSGYLEQVEALVEGGIDVILVETVFDSLNCKAALFAIEEFFKTYSPRLPVFVSGTIVDKSGRTLSGQTGEAFYTSVASANLMVFGLNCALGAQEMRPFLQNISKCSECYVSCYPNAGLPNTFGGYDETPEMMAEQIKEFAESGLLNIVGGCCGTSPDHIRAFCNAMGLKQFCQELGERCNVSGSRRFANLIKANKYEEALSVARQQVEAGAQIIDINMDEGMIDAVAAIQKFLFFIGSEPEISKVPIMLDSSNFDVVEAGLKCVQGKCIVNSISLKVGEELFIKQAKIVKQYGASVVVMAFDENGQATSKEEKVRICYRSYKILTEQVGFYPQDIIFDPNILTIATGLEEHNNYGVEFIEATREIKALMPLTRVSGGVSNLSFSFRGNEPLREAMHSAFLYYAIAAGMDMGIV",
    "response#3": "MVLDGAMGTEIQKFKLKDNDYRGEEFKDFPHELGGNNDLLSLTQPHIIREIHCKYLEAGADFIETNTFNGNIFSQADYKMEHLVKRINIESARLAKSACEEYTKKDPSRPRFVCGAVGPTNKTASISPSVERPEARNVLFDELVSGYLEQVEALVEGGIDVILVETVFDSLNCKAALFAIEEFFKTYSPRLPVFVSGTIVDKSGRTLSGQTGEAFYTSVASANLMVFGLNCALGAQEMRPFLQNISKCSECYVSCYPNAGLPNTFGGYDETPEMMAEQIKEFAESGLLNIVGGCCGTSPDHIRAFCNNHTLGFPRVGLKRELKKAQESYWAGNSTQEELLNVGRELRARHWQQQQQAGVDLVPVGDFAWYDHVLTTSLLLGNVPERHQNADGSIDIDTLFRIGRGRAPTGKPAAAAEMTKWFNTNYHYMVPEFQQGQQFKLGWTQLLDEVDEALALGHKIKPVLLGPITYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQEWLDAYQPAYQALQGQVKLLLTTYFDSIGHNIDTIRALPVQGLHVDVVAGHDDLAVLHKNLPKEWLLSLGVINGRNVWRADLSSWFERLQPLVTTSLPYPNREGEQEVEEWRNKPVSERIAHALVKGITTYIIEDTEEARNTLPSSLSVIEGPLMGGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLIPFMEEEKRLKL"
  },
  {
    "reference": "MRKRHRTKYIFVTGGVVSSLGKGIVAASLGSLLESRGLRVSMTKVDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDIGHYERFTSATLTRLNSFSTGQIYDKVLSKERRGDYLGGTVQVIPHVTDQIKENIYAGSEGADVAIVEIGGTVGDIESLPFLEAIRQIRYDLGDENVVYIHLTLVPYINAAHELKTKPTQHSVKELRQIGIQPNILICRADRPVPAELREKIGLFCNVKPERVIDCPDLDTIYKVPLYLHAQGLDQTIVDALNIWTRNPELSEWHRIQETLDRPTGHVKIGVVGKYIDVIDSYKSINESLIHAGISNQVKVETIYFDAATLNHDNVAQHLKGLDGVIVPGGFGNRGIEGKIAAIRYLRENKVPFLGICLGMQLAAIEFARHKLGLSIADSQEFNAQSPDQIIHLMEDQKKLKDLGGTMRLGAYPCQLQVGSKAQEAYGEEAISERHRHRYEFNNAYRQRFEEAGMRFSGLSPDGSLVEVIELKDHPWFVGSQFHPELKSKPMQPHPLFRDFVGKAVEGRNA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein with glutaminase activity, metal ion binding, CTP synthase activity, ATP binding is needed to enhance glutamine metabolic process, 'de novo' CTP biosynthetic process in a specific context.2. The designed protein should contain one or more Glutamine amidotransferase, CTP synthase N-terminal domains that are essential for its function.3. The design of the protein must focus on the precise arrangement of amino acids in the active site, Nucleophile, Nucleophile; for glutamine hydrolysis to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).4. The protein should be able to bind CTP, Mg(2+), L-glutamine, ATP ligand in a variety of conditions5. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen6. A protein should be able to manipulate the activity of enzymes in the Pyrimidine metabolism, CTP from UDP: step 2/2, CTP biosynthesis via de novo pathway pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "KFIFVTGGVVSGLGKGITASSLGALLKASGLKVFMQKFDPYLNVDPGTMSPYQHGEVFVTSDGGETDLDLGHYERFTDEELSKISSTSAGKIYLEVIEGERRGDSQGKTIQVVPHITDAIKHKVYLAAQRSKADVIISEIGGTVGDIESQPFIEAIRQIRMEQGKENVMFIHVVLLLWLAASKEYKTKPIQHSVRELLSLGIQPDVIVSRSDKSSPQEIKEKVSLFCNVPTENIIDAVDQDSIYRVPLAMAEQNLHKLVIDQLKVNIAMCGKYVGLKDSYKSISEAFVHAGVANDAHVELHWIDTEKVEQNGVPPEFKNMHGLVVLPGFGSRGVEGKIKMIQTAREQKVPFLGICLGMQCAVIEFSRNVCGLQANSTEFEEQTPHPVIDKMETQVNIKQMGGTMRLGAYRCELKAGTRAHAAYRESVIYERHRHRWEVNNRYLPQVQERGYKVSGINPETKLVEIMELENHPWFVGVQFHPELRSRALRPHPLFRDFIRACTAQ",
    "response#2": "MTKYIFVTGGVVSSLGKGIVTSSLGAILRARGYRVTAVKIDPYINVDAGTMRPYEHGEVFVTADGAETDLDIGHYERFLDRDLSRGNNITTGQVYLSVIQKERRGEYLSQTVQVIPHITDEIKRRIRQVAEAQQAEIVVVEVGGTVGDIESLPFLEAIRQFRFDEGPEDTLYIHLTLVPYLRTSEEFKTKPTQHSVATLRGVGIQPDVIVVRSERPLPPEVRKKIALFTNVHPDEVFSSHDVEHLYEVPLILEEQGLGKTVERKLGLERPLADLRAWTELLDRVHHPKHEVTVALVGKYISLHDAYISVVEALKHGGVANHASVSIRWVDSEEVNDGNAAEIFDGVNGILVPGGFGSRGIEGKISAIRWTRTHGIPFLGLCLGMQMAIVEFARDVVGYSDAHTAELDPDTGHPVIHLMPDQNGVEDIGGTLRLGSYPCVLDKTSRAYALYGQETIHERHRHRYEVNNDYRQALTENGMKLSGISPDGRIVEMIELPEHPWFVATQAHPEFKSRPNRPHPLFAGFVRAALERRKAPLA",
    "response#3": "RFIFITGGVVSSLGKGLGSAALAALLQARGYSVRXRKLDPYLNVDPGTMSPTQHGEVFVTDDGAETDLDLGHYERFTGVHTRKTDNVTTGRIYSDVLARERRGDYLGATXQVIPHVTDAIKAFVRNDLSAEDFVLCEIGGTVGDIESLPFLEAIRQIGNELGRSQVCFIHVTLIPYIKAAGELKTKPTQHSVKELQSLGIQPDIXLCRTEHXLEREXRDKIGLFCNVRSEAVILASDVNNIYEVPVAYHNEGLDTQVCAHFGLKTPPADLSHIESVIYNETHRSSVTVALVGKYISLHDAYISVVEALKHGGVANHASVSIRWVDSEEVNDGNAAEIFDGVNGILVPGGFGSRGIEGKISAIRWTRTHGIPFLGLCLGMQMAIVEFARDVVGYSDAHTAELDPDTGHPVIHLMPDQNGVEDIGGTLRLGSYPCVLDKTSRAYALYGQETIHERHRHRYEVNNDYRQALTENGMKLSGISPDGRIVEMIELPEHPWFVATQAHPEFKSRPNRPHPLFAGFIQAAGKHA"
  },
  {
    "reference": "KLSGCFGFKLDRIGTMSGLGC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Scorpion venom natriuretic peptide that increases the perfusion pressure, glomerular filtration rate and urinary flow in the isolated perfused rat kidney assay2. The protein must have high specificity towards hormone activity. The designed protein sequence is ",
    "response#1": "CNLPVKPGPCNGFFSAFYYSQKTNKCHSFTYGGCKGNANRFSTIEKCRRTG",
    "response#2": "SFCNLPVKPGPCNGFFSAFYYSQKTNKCHSFTYGGCKGNANRFSTIEKCRRTCG",
    "response#3": "CNLPVKPGPCNGFFSAFYYSQKTNKCHSFTYGGCKGNANRFSTIEKCRRTC"
  },
  {
    "reference": "MMSSAVCRLAPSPTGAQHLGNARTFLIAYWSARAQNARLILRIEDIDSPRIKPWATEQAITDLRWLGMDWDGDPIVQTERSPLYDEARNQLMDADRVYPCSCTRRDIEAAASAPHESEASGWTPDAPVYPGTCASWKNGDPLPEDKPFCFRFRMQATPDRFFDRVHGEVACNPIQDIGDFPITRKAESAADCLAAYQLAVVVDDIDAGVTEVVRGDDLILSTFRQLQLYEALGHSPPSHAHVPLVTGTDGRRLAKRHGDTRLSHFREQGMPPETIVRWAAKSSGLCPDSDAALSERTLDQIHQQMIDRFDWVRIHRQPTVVDDFGSTTD",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that contributes to tRNA modification, glutamyl-tRNA aminoacylation by functioning in cytosol with ATP binding.2. The protein must exhibit the following characteristics: Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2- cyclopenten-1-yl) moiety of the queuosine in the wobble position of the QUC anticodon.; Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp).3. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity.4. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects.5. The protein's functional design necessitates the inclusion of a 'KMSKS' region motif. The designed protein sequence is ",
    "response#1": "RLLEVLQPFWKQLGIEAPDPDYGRSIVRDLRARAKTLVDMAESSTFYFNDEPAIDADAAKKFLTPEIAGHLEAIAEALATLGDYSKEGIEIFLRSLVEARAIKLKTIAQPLRIALTGKTVSPGLDDIMLTLGKERVIARIQRR",
    "response#2": "RGRFAPSPTGPLHFGSLVGALASWLDARAHGGVWLVRIEDLDGPRTVPGAADDILATLAHFGMTPDEPPVWQSTRDAAYTAALERLVAAGLVYPCGCTRKEIADSLRAAHERHTTLAYPGTCRTGLHGKPARAWRLRVPDGCDAVITFDDRWQHTQSQNLATEVGDFVLKRADGQWAYQLAVVVDDADAGITHVVRGADLLDSTARQIYLQRCLGVPTPEYLHVPVVVDANGEKLSKQTGATW",
    "response#3": "VIGRFAPSPTGPLHVGSLVAAVASYAMARRQGGLWLVRMEDLDTPRVVPGMADDILRTLECLGFDWDGDIMRQSRRADAYGAALQRLLAAGHAYPCGCSRAEIARAATAPHDGDGEIPYPNLCRRGLPPGKEPRSFRVRVPAEPVEFTDLVMGPQHHDLPAMCGDFVIKRADGLFAYQLAVVVDDEAQGVTQVVRGADLLSSTPRQIVLQRLLGFDTPVYAHVPLVTGPGGGKLSKRDNALSLAAGRDLTREGGMLLLAALRFLG"
  },
  {
    "reference": "MSFLKKSLLLILFLGLVSLSVCKEEKRETEEENENEENHEEGSEMKRYMFHLMDGEAKKRDSEENEIEENHEEGSEMKRYAFGYPSGEAKKIKRVSEEENENEENHEEGSEMKRYAFGYPSGEAKKIKRESEEEKEIEENHEEGSEMKRYAFGYPSGEAKKIKRESEEENENEENHEEGSEMKRYAFGYPSGEAKKM",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Ensure the presence of a signal peptide within the protein.2. The protein design should be able to enhance the efficiency of defense response.3. The protein must exhibit the following characteristics: It has high affinity and selectivity for delta-type opioid receptors.; Deltorphin has a very potent opiate-like activity. The designed protein sequence is ",
    "response#1": "NGDMCNLSFYKNEICFQPNGFLIEDILQNWKDNYDLLEENHSYIQWLFPLREPGVNWHAKPLTLKEVEAFKSSKEVRERLVRAYELMLGFYGIQLEDRNTGAVCRAQNFQPRFHNLNSHSHNNLRITRILKSLGELGLEHYQAPLVRFFLEETLVQHKLPSVRQSALDYFLFAVRCRHQRRELVHFAWEHFKPRREFVWGPRDKC",
    "response#2": "KAQEGTVCLRSSDCAAGLCCARHFWSKICKPVLKEGQVCTKHRRKGSHGLEIFQRCYCGEGLSCRMQKDHHQASNSSRLHTCR",
    "response#3": "NGDMCNLSFYKNEICFQPNGFLIEDILQNWKDNYDLLEENHSYIQWLFPLREPGVNWHAKPLTLKEVEAFKSSKEVRERLVRAYELMLGFYGIQLEDRNTGAVCRAQNFQPRFHNLNSHSHNNLRITRILKSLGELGLEHYQAPLVRFFLEETLVQHKLPSVRQSALDYFLFAVRCRHQRRELVHFAWEHFKPRREFVWGPRDKC"
  },
  {
    "reference": "MASPTLGASRGSPDALPAGSASQEDEEGDSAGWTGSSGSCASASGSSDDLEPEWLDSVQKNGELLYLELSEGEEESLLPETPTVNHVRFSENEIIIEEDDYKERKKCEPKLKRFTKILKSKRLLPKRNNKKNSSDNGPVSILKHQSNQKTGVIIQQQYKDVNVYVNPKKLTVTKAKEQLRLLEVLVGIIHQTKWSWRRSERQGDGERLVVHGLSPGGSAMKSGQIVVGDVLVAVNDVDVTSENIERVLSCIPGPMQVKLTFENAYAVKKETNQLRKKKAQSNTNDLVKLLWGEEAGSIPQNMVDSPHIIMYLTLQLDSEISKEEQEILYHYPMSEASQKLKSVRGIFLTLCDMLENVTGTQVTSSSLFLNGKQIHVAYLKQSDKLLLIGLPAEEVPLPQLRNMIEDVAQTLTFMYGSLDRSFCQVENAARLDHFFTLFFQRALQPASPPPCASPSAWQYEASSAALLDNLPGVRWLTLPREIKVELDTALSDLEAADFAELSEDYYDMRRLYTILGSSLFYKGYLICSHLPKDDLVEIAAYCRHYGLLSLAAKQRIGQLIVWREVFPQHHLHLSADSSTEAFQEPEGRYFLLIVGLRHYMLCVLLEAGGCASKAIGNPGPDCIYVDQVRTTLHQLEGVDSHIDEHLASSPGPCLSCADWFLSGTREKTENLTASPILSRLQGPSRTAASPTCRRTLFGDYSLKTRKPSPSRSSGGSDNGCEGGEGAGLSPHTTLDAIQKQRDFPGSDCSEESGTLLKITKKKSTLPNP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The presence of PDZ domains is critical for the interaction of the designed protein with its intended targets.2. The protein should exhibit Polar residues compositional bias.3. The protein should be engineered to have a higher affinity for a specific molecule or receptor in ciliary transition zone, centriole, cell surface, motile cilium, cytoplasm that is critical for protein localization to organelle, negative regulation of keratinocyte proliferation, negative regulation of cell division, neural tube development, roof of mouth development. The designed protein sequence is ",
    "response#1": "PHIIMYLTLQLDSETLKEEQEILYHYPKSEASQKLKSVRGIFLTLCDMLENVTGTQVTSSSLLLNGKQVHVAYWKESDKLLLIGLPTEEVPLPWLRNMIKDVAQTLKFMYC",
    "response#2": "ILGSSLFYKGYLICSHLPKDDLIDIAVYCRHYGLLPLAAKQRIGQLVIWREVFPRHHLQPSTDSNTEVFQEPEGRYFLLIVGWRHYMLCVLLEAGGCSSKAIGNPGPDCIYVDQGKTTC",
    "response#3": "ILGSSLFYKGYLICSHLPKDDLIDIAVYCRHYGLLPLAAKQRIGQLVIWREVFPRHHLQPSTDSNTEVFQEPEGRYFLLIVGWRHYMLCVLLEAGGCSSKAIGNPGPDCIYVDQGKTTC"
  },
  {
    "reference": "MATRWERAQRLDEEDPLAHVRKHFALPDDVCYLDGNSLGALPRGVGDRVREVVDEGWGKQLIRGWDGAGWWEAPERIGDRIGGLLGAAAGQTVVGETTTVQIFNALVAAARLRPDRELIVTDSDHFPTDRYLAASVARMLGKRLLLVPVAEAPALLRERAAEIAVLALPAVDFRTGELWPLRELTAEAHGAGAVVMWDLCHAVGAMPVDLDAAEVDVAVGCSYKFLSGGPGAPAFIYVAERHHSRLDLPLTGWTGHAEPFAMAAEYQPAPGIARARVGTPHVLSLLALEAALAVFDLVELAAVRAKSVALGNFLIECAESELVPLGFEVVTPCDESRRGSQVTLRHPQAYELVRLLAGRGVIGDSRPPNLLRFGLNALYVSYQDVHRAVQELAALAR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein localizes to the cytoplasm.2. The presence of Aminotransferase class V domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "NSMGLKPKNVHELLHIVLQQWGKLGVHAYHRGPLPASHCDIQLTKDIAELIVGAEPDEVAITGGLSTNIHLLFSSFYRPHGVKTCILIEAGAFPSDYYVVESQISWHGLNPSRCIIQIKPRPGEWCLRTEDVIQKIREEAEKLALIWLPGVQYFTGQLFDMEQITQAGHQYANCPVGWDLAHAVGNVPLELHKWNVDMAAWCSYKYLNGSPGAIAGLFVHRKHHHQH",
    "response#2": "NSMGLKPKNVHELLHIVLQQWGKLGVHAYHRGPLPASHCDIQLTKDIAELIVGAEPDEVAITGGLSTNIHLLFSSFYRPHGVKTCILIEAGAFPSDYYVVESQISWHGLNPSRCIIQIKPRPGEWCLRTEDVIQKIREEAEKLALIWLPGVQYFTGQLFDMEQITQAGHQYANCPVGWDLAHAVGNVPLELHKWNVDMAAWCSYKYLNGSPGAIAGLFVHRKHHHQN",
    "response#3": "NSMGLKPKNVHELLHIVLQQWGKLGVHAYHRGPLPASHCDIQLTKDIAELIVGAEPDEVAITGGLSTNIHLLFSSFYRPHGVKTCILIEAGAFPSDYYVVESQISWHGLNPSRCIIQIKPRPGEWCLRTEDVIQKIREEAEKLALIWLPGVQYFTGQLFDMEQITQAGHQYANCPVGWDLAHAVGNVPLELHKWNVDMAAWCSYKYLNGSPGAIAGLFVHRKHHHQY"
  },
  {
    "reference": "MIWLFLFGALGLIYTMTVGVPKNATRVPFSQLIKDIDEKGNVKKLVIHGEEYQWTYHDGQSFYAVGPIADDKLNTLFLKNKIEVQYKPHTNKSSFWQVILISWLPILLLLGFLFFMLRQLQGGRGPDPNGFGKINLRGDPSHRSKVTFDDVAGIDEVKEELVEIVEFLRDPKKYTRLGGRIPKGVLLMGAPGTGKTLLARSVAGEADVPFFSISGSDFVELFVGVGASRVRDLFEQAKKSAPCIIFIDEIDAVGRHRGIGLGGGNDEREQTLNQLLVEMDGIHSNEGVIIVAATNRSDILDPALQRPGRFDRQVMVPLPDVIGREAILHVHSKRIPLAEGTNLRDIAKGTPGFSGAELENLVNEAALFAAREDRDRVYTEHFEKAKDKVIMGQERRSMMLSEEEKEHTAVHEAGHALVGKLLEHCDPVHKVSIIPRGRALGVTMTLPDDDRKDVFEDYLYDLMAFAMGGRAADELIYNHKTAGASDDIRRVTGIARKMVCEWGMSEKLGPLSYKTGSDEPLVGRDISQGRPYSETTAQMIDEEVSRLVNDSYKKAVQLLSDNIDMLKTVSAALVERENISGDELDRLMRGESLPPLPLPPRSKGNETSEKSNSDAVAPIAVPEAG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein have AAA+ ATPase domains with specific sequence motifs that are crucial for its activity.2. The protein should contain a Helical membrane-spanning region.3. The active site active site of the designed protein must be optimized for substrate binding and specificity.4. A protein that contributes to proteolysis, cell division, protein catabolic process by functioning in plasma membrane with metalloendopeptidase activity, ATP hydrolysis activity, ATP binding, ATP-dependent peptidase activity, zinc ion binding.5. The protein should have a high affinity for Zn(2+), ATP.6. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity.7. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins; Plays a role in the quality control of integral membrane proteins. The designed protein sequence is ",
    "response#1": "MVMSEKEKLNTAYHESGHAIVGRLMPEHDPVYKVSIIPRGRALGVTMFLPEEDKYSHSKRYLDSSICSLFGGRIAEELTLGFEGVTTGASNDIERATSLARNMVTRWGLSEKLGPLQYDTDSDEPFLGRSAGQAQTVYSPETAQRIDEEVRNIIDTCYEKAKQILVDNRDKLDLMADALMKYETIDRFQIDDDVIEAFRAAQANG",
    "response#2": "MVMSEEEKKLTAYHEGGHALVALHMEDSDPVHKATIIPRGRALGMVMRLPERDQLSVTRSKLKADLAVAMGGRVAEEVIFGHDKVTSGASSDISMATKMAKAMVTQWGMSDKLGPLAYQDNEEEVFLGHSVARQQTMSEDTARMIDEEVKRIVSEGYEKAQELLTKHLDELHTLAQGLLEYETLSGEEIKAAQDELARRARVEARAREMLEREAREADARAEAEAAAAREAAAAREAAAAREAAAAREEAAARDAAARDAAARPAATEPAAAAVPAADAAPEIATAPAEAAAPVAEAAPAEAAPAEAAPAEAAPAEAATAEAAPAEAAPAEAAPAEAAPAEAATAEAAPAEAAPAEAAPAEAAPAEAATAEAAPAEAAPTEAAAAEAAPAEAAAAEAAPAEAAAPAEAAAPAEAATAAAAPAEAAPAEAAPAEAATAEAAPAEAAPAEAAAAEAAPAEAAPAEAAAAEAAPAEAAAAEAAPAEAAAPEAAPAEAAAAEATPAEAAPAEAAAAEAAPAEAAAAEAAPAEAAAPEAAPAEAAPAEAAAAEAAPAEAAAAEATPAEAAPAEAAAAEAAPAEAAAAEAAPAEAAAPEAAPAEAAAAEAAPAEAAAAEAAPAEAAPAEAAPAEAAAAEAAPAEAAAAEAAPAEAAAPEAAPAEAAAAEAAPAEAAAAEAAPAEAAAPEAAPAEAAPAEAAAAEAAPAEAAAAEAAPAEAAAPEAAPAEAAAAEAAPAEAAAAEAAPAEAAPAEAATAE",
    "response#3": "MVMSEEEKKLTAYHEGGHALVALHMEDSDPVHKATIIPRGRALGMVMRLPERDQLSVTRSKLKADLAVAMGGRVAEEVIFGHDKVTSGASSDISMATKMAKAMVTQWGMSDKLGPLAYQDNEEEVFLGHSVARQQTMSEDTARMIDEEVKRIVSEGYEKAQELLTKHLDELHTLAQGLLEYETLSGEEIKALQAELYRARGVLMVGQPGTGKTLLAKAIAGEAKVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKSAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQMLVEMDGFEGNEGVIVIAATNRPDVLDPALLRPGRFDRQVTVDFVDLSLVAKSTPGASGADLANIINEAALRAVRMGRRRVTTEDLLALIARGTPGFSGADLANLVNEAALFAARRNQRLVSMEEFEFGVQYLHEHSFMPEPPDA"
  },
  {
    "reference": "MKLVVGLGNPGKKYEHTRHNIGFDIIDRCAQKLDLPLDKQKFKAIYGEKRIGSEKVVLLKPLTYMNLSGEAVRPFMDYFNISNDELVVIYDDLDLPVGKIRLRKKGSAGGHNGIKSLIAHLGTEDFNRIRVGIGRPAHGEPVVHYVLDTYRKEELAFMNEAAEKSAKAIEAWLEKPFLEVMNTFN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein have enhanced an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-   amino acid + H(+).2. The protein should have aminoacyl-tRNA hydrolase activity and be active in cytoplasm.3. The protein must exhibit the following characteristics: The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.. The designed protein sequence is ",
    "response#1": "MTPTF",
    "response#2": "GAAEFL",
    "response#3": "SILG"
  },
  {
    "reference": "MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVRTVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGENRYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEPQLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTEAKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRIYDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRGGIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGISQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Together with LptE, is involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane; Contributes to n-hexane resistance..2. The protein should be optimized for stability and expression in transporter complex where it will participate in lipopolysaccharide transport.3. The engineered protein is expected to adopt a strand-turn-strand-strand-strand-strand-helix-strand-strand-helix-strand-strand-turn-strand-helix-based conformation. The designed protein sequence is ",
    "response#1": "NFLGTGAAVSIAGTKNDYGTSVNLGYTEPYFTKDGVSLGGNVFFENYDNSKSDTSSNYKRTTYGSNVTLGFPVNENNSYYVGLGHTYNKISNFALEYNRNLYIQSMKFKGNGIKTNDFDFSFGWNYNSLNRGYFPTKGVKASLGGRVTIPGSDNKYYKLSADVQGFYPLDRDHLWVVSAKASAGYANGFGNKRLPFYQTYTAGGIGSLRGFAYGSIGPNAIYAEYGNGSGTGTFKKISSDVIGGNAIATASAELIVPTPFVSDKSQNTVRTSLFVDAASVWNTKWKSDKNGLESDVLKRLPDYGKSSRIRASTGVGFQWQSPIS",
    "response#2": "NFLGTGAAVSIAGTKNDYGTSVNLGYTEPYFTKDGVSLGGNVFFENYDNSKSDTSSNYKRTTYGSNVTLGFPVNENNSYYVGLGHTYNKISNFALEYNRNLYIQSMKFKGNGIKTNDFDFSFGWNYNSLNRGYFPTKGVKASLGGRVTIPGSDNKYYKLSADVQGFYPLDRDHLWVVSAKASAGYANGFGNKRLPFYQTYTAGGIGSLRGFAYGSIGPNAIYAEYGNGSGTGTFKKISSDVIGGNAIATASAELIVPTPFVSDKSQNTVRTSLFVDAASVWNTKWKSDKNGLESDVLKRLPDYGKSSRIRASTGVGFQWQSPIR",
    "response#3": "NFLGTGAAVSIAGTKNDYGTSVNLGYTEPYFTKDGVSLGGNVFFENYDNSKSDTSSNYKRTTYGSNVTLGFPVNENNSYYVGLGHTYNKISNFALEYNRNLYIQSMKFKGNGIKTNDFDFSFGWNYNSLNRGYFPTKGVKASLGGRVTIPGSDNKYYKLSADVQGFYPLDRDHLWVVSAKASAGYANGFGNKRLPFYQTYTAGGIGSLRGFAYGSIGPNAIYAEYGNGSGTGTFKKISSDVIGGNAIATASAELIVPTPFVSDKSQNTVRTSLFVDAASVWNTKWKSDKNGLESDVLKRLPDYGKSSRIRASTGVGFQWQSPIV"
  },
  {
    "reference": "MRVRETQRNWLNLWRWGTLILGLVIMCSASDNLWVTVYYEVPVWRDADTTLFCASDAKAHSTEMHNVWATHACVPTDPNPQEIYLVNVTENFNMWKNRMAEQMQEDVISLWDQSLKPCVKLTPLCVTLNCSNVNWTKANVTDNGTNNIGNITEEVKNCTFNMTTELRDKKEKVYALFYKLDIVQIRDDNSTYRLINCNTSVIRQACPKISFDPIPIHYCAPASYAILKCNDKNFNRKRPCKNVSSVQCTHRIRPVVSTQLLLNGSLAEDKIIIRSENLTNNAKTIIVHLNKSVEINCTRPSDKIRTSTQIGPGQVFYRTGSILGDIRKAYCEVNRTQWSKVLKQVTENLKEHFNKTIIFQPPSGGDLEITMHHFTCGEEFFYCNTTNLFNNSCTTERCNGTIILPCKIKQIINMWQEVRQAMYAPPISRKINCVSNITGILLTRDSSNNNSIGNETFRPRGGNIKDNWRNELYKYKVIQIEPLRVAPTKARRRVVKREKRAVRIGAMIFKFLRAAGSTIGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSRKIICTTSVPWNHTWSNKSYEQIWDNMTWIKWEREISNYTNQIYQLLTESQDQQDRNEKDLLELNKWASLWNWFDITNWLWYIKIFIMIVRGLIKLRIIFAVLSIVNKVKQRYSPLSFQTPIHHQKEPDRPERIEERGGEQGRERSVRLVSRFLALVWEDLQSLCFFLYHRLRDFTLIAA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that can perform structural molecule activity in host cell plasma membrane, virion membrane to support clathrin-dependent endocytosis of virus by host cell, fusion of virus membrane with host endosome membrane.2. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.3. The protein must contain a signal peptide for proper functionality.4. For general function, the protein need meet that The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane5. The designed protein should contain Cytoplasmic topological domains. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPSPQEVVLENVTENFNMWKNNMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDYDYEKNWTSYTNATWEQKEEIKNCSFNITTSISDKKKQEHALFYRLDVVPIETTNNASNNSYYRLINCNTSIITQACPKVSFEPIPIHYCAPAGFAILKCNNKTFNGSGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEKEVVIRSANFSDNAKVIIVQLDVPVQIICSRPNNNTRKSTHIGPGAALYTTGEIIGDIRQAHCNVSSQNWTATLKKVVPKLGDQFGNKTIVFNQSSGGDPEIVMHTFNCGGEFFYCVTTKLFNSTWTFNSTNGTWTLNSTWNSTEGLNNNDTIILPCRIKQIINMWQTVGKAMYAPPIRGPINCTSNITGLLLTRDGGKETNTTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKARRRVVQREKRXXXXXXX",
    "response#2": "MTEEIKNCSFNATTELRDKSRKEYALFYRLDVVPINGDRNSSEYRLINCNTSTITQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIMIRSENLTNNAKTIIVQFKYPVRIVCTRPNNNTRKSVRIGPGQTFYATGGIIGDIRQAHCNISEKNWTSTLQEVVKKLQEHFPNRTINFNSSSGGDLEITTHSFNCRGEFFYCNTSKLFNSTHNVTDYNDTNSSSNSTITLPCRIKQIINMWQEVGKAMYAPPIAGNITCISNITGLLLTRDGGNSSDNNNTEIFRPAGGDMRDNWRSELYKYKVVEIKPLGLAPTKAKRRVVERRQKR",
    "response#3": "DVKVCAFNVTTEIKDKKRKVHALFYKLDIVPLNDKDKNSSEYRLINCNTSVIKQACPKISFDPIPIHYCTPAGYAILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEKEIIIRSENLTNNAKTIIVHLNKSVEINCTRPSNNTRKSINIGPGQVLFYRPGDVIGNIRQAYCEINGTKWNETLRQVTGKLKEHFKNKTINFQPPPGGDLEITMHHFNCRGEFFYCNTTQLFNGVYNNDTKNRTRGSNDTITIPCKIKQIINMWQGIGQAMYAPPISGTINCVSNITGILLTRDGGDSTNETFRPGGGNIKDNWRSELYKYKVVQIEPLGIAPTRAKRRVVEREKR"
  },
  {
    "reference": "MTDKTFLVEIGTEELPPKALRNLAESFAANFTAELDAANLAHGEVSWFAAPRRLALKVARLSASQPDREVEKRGPAIAQAFDAEGKTTKAAEGWARGCGITVEQAERLTTDKGEWLLYRAHAKGEQAQALLAGMISTALSKLPIPKLMRWSDKETQFVRPVHTVTMLLGDELIPGQVLGIHSARTLRGHRFMGEAEFTIDSADQYPQILLERGKVVADYDARKAKIKADAEEAARKIGGNADLSDSLLEEVTSLVEWPVVLTAKFEEKFLAVPSEALVYTMKGDQKYFPVYDNSGNLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFFNTDRKKRLEDHLPRLETVLFQQQLGTLRDKTDRIQALAGWVAGQIGADVNHATRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDELPSSAVACALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKRLPLDLQTLTEEAVRLYGAKLTNANVVDDVIEFMLGRFRAWYQEEGHSVDTIQAVLARRPTRPADFDARVKAVSHFRSLDAAAALAAANKRVSNILAKSTDTLNESVNAAVLKDAAEITLATHLVVLRDKLTPLFAEGRYQEALVELASLREPVDAFFDQVMVMAEDEQVRVNRLTLLSQLRELFLQVADISVLQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a fast turnover rate for ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-   tRNA(Gly).2. The protein must be able to arginine-tRNA ligase activity, ATP binding. The designed protein sequence is ",
    "response#1": "MLRVSNILAKADVAIGEVNPTACVEPAEKALAEAVLGLRTEVQPLIAKGEYTAVLDKLASLRQPVDSFFDNVMVNAEDPALRQNRLAILNTLQGLFLQVADISLI",
    "response#2": "RVSNILAKADVAIGEVNPTACVEPAEKALAEAVLGLRTEVQPLIAKGEYTAVLDKLASLRQPVDSFFDNVMVNAEDPALRQNRLAILNTLQGLFLQVADISLV",
    "response#3": "MTRANNILTQAEEADGVEYSYGADPKFAETDEERALFAALDTAEAVIDPALEAEDFAAATAAMASLRAPLDAFFEAVQVNAENQILRRNRLNLLSRIRATCLKLADLTRR"
  },
  {
    "reference": "MYSSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKAEVKQKWASRLLAKLRKDIRPECREDFVLAVTGKKAPGCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKAAACAHPVLCVQPHHIGVAVKELDLYLAYFVRERDAEQSSSPRTGVGSDQEDSKPITLDTTDFQESFVTSGVFSVTELIQVSRTPVVTGTGPNFSLGELQGHLAYDLNPASAGMRRTLPSTSSSGSKRHKSGSMEEDVDTSPGGDYYTSPNSPTSSSRNWTEDIEGGISSPVKKTEMDKSPFNSPSPQDSPRLSSFTQHHRPVIAVHSGIARSPHPTSALHFPATPILPQTASTYFPHTAIRYPPHLNPQDPLKDLVSLACDPATQQPGPPALRPTRPLQTVPLWD",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should exhibit Polar residues compositional bias.2. The protein must exhibit the following characteristics: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2.3. The CTF/NF-I domain within the designed protein should exhibit high affinity and specificity towards its target DNA sequence.4. The designed protein must possess RNA polymerase II cis-regulatory region sequence-specific DNA binding to support negative regulation of transcription by RNA polymerase II under certain conditions. The designed protein sequence is ",
    "response#1": "MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQKWASRLLAKLRKDIRPEFREDFVLTVTGKKPPCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKSPQCSNPGLCVQPHHIGVSVKELDLYLAYFVHAADSSQSESC",
    "response#2": "MGSRLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKAEVKQKWASRLLAKLRKDIRPECREDFVLAITGKKAPGCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKAAQCGHPVLCVQPHHIGVAVKELDLYLAYFVRERGEAGRARR",
    "response#3": "MGSRLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKAEVKQKWASRLLAKLRKDIRPECREDFVLAITGKKAPGCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKAAQCGHPVLCVQPHHIGVAVKELDLYLAYFVRERGEAGRARL"
  },
  {
    "reference": "MDSEASDIEAELSDSVSAGGEEYIDDDDYTEDIDDQIVTAKSSRRTARRSVPKGVRTSKRIRDKELSVEVDEDYDEEEDVLSPSKKRHLHTRSMDKRQVAATASEKSDIGDSKGNDGEIEDGILEEEESLEKELNRGGGKEVEKSEESYYAQNDVGQKGEEEQDGESGGYEDNEPSISKESDELVSVVNGNGNEEDDEVEATKENTTDSTRSTTTRSKMLLDLLEDGGSKKKLTDEEIQLRRAENARKRKNLSEKRLEEEKQDTINKLLKKRAGKSRSHLPNDDEKNDGSSSFVKPRRPYNSEGMTRILRRYEEDLFCTF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be expressed specifically in the Ino80 complex The designed protein sequence is ",
    "response#1": "QTPSSVSAAVGGTVTINCQSSQSVYENGRLSWFQQKPGQPPKRLIYRASTLASGVSSRFTGSGSGTQFTLSISDVQCDDAATYYCLGNYDCSSGDSFTFGGGTEVVA",
    "response#2": "SHSLRYFYTALSRPGLGEPRFIVVGYVDDTQFVRYDSDVENPRMEPRARWMEQEEPAYWERETRKARDTGRNFKVNLRTLLRYYNQSDDESHTLQWMYGCDVGPDGHLLRGYCQEAYDGRDYISLNEDLRSWTATDMASQASKIKSEEVGEAHHQRAYLQGPCVEWLHTYLHLGKETN",
    "response#3": "PSSVSAAVGGTVTINCQSSQSVYENGRLSWFQQKPGQPPKRLIYRASTLASGVSSRFTGSGSGTQFTLSISDVQCDDAATYYCLGNYDCSSGDSFTFGGGTEVVVL"
  },
  {
    "reference": "MTTTVIIIIFVAIFATTLHDARGATMEPCLESMKTASLPDLPYAYDALEPAISEEIMRLHHQKHHQTYVTQYNKALNSLRSAMADGDHSSVVKLQSLIKFNGGGHVNHAIFWKNLAPVHEGGGKPPHDPLASAIDAHFGSLEGLIQKMNAEGAAVQGSGWVWFGLDRELKRLVVETTANQDPLVTKGSHLVPLIGIDVWEHAYYPQYKNARAEYLKNIWTVINWKYAADVFEKHTRDLDIN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be involved in floral organ abscission by localizing to mitochondrial matrix, mitochondrion and exhibiting manganese ion binding, superoxide dismutase activity.2. The protein should be designed to catalyze the following reaction 2 H(+) + 2 superoxide = H2O2 + O2.3. The protein must be engineered to have a cooperative binding mechanism with Mn(2+) to ensure proper regulation.4. The protein should have a high affinity for Mn(2+).5. The protein must exhibit the following characteristics: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.. The designed protein sequence is ",
    "response#1": "VGELAAAIDDQFGSFDKFRAQFTAAANGLQGSGWAVLGYDTLGQKLLTFQLYDQQANVPLGIIPLLQVDMWEHAFYLQYKNVKADYVTAFWNVVNWADVQDV",
    "response#2": "VGELAAAIDDQFGSFDKFRAQFTAAANGLQGSGWAVLGYDTLGQKLLTFQLYDQQANVPLGIIPLLQVDMWEHAFYLQYKNVKADYVTAFWNVVNWADVQDV",
    "response#3": "SLAAEIEKTWGSFDEFKKAFSAALLGIQGSGWGWLVKESTAEKGRLRIITTKDQDPVVGGEVPVFGVDMWEHAYYLQYLNGKAAYVENIWKVINWKTAEEA"
  },
  {
    "reference": "MSHTKIMSTDQLETQITELDNLPEHREIVTFMRRSAPLNTSQRSALEQYQDLILEYPVGDLRQHFEHPEHPLTVEIGFGMGRSLVLMAKAHPERNFVGIEVHVPGIAQCVYEAGMAGLKNLRVLDADAIQVLREMPDNSINCVQLYFPDPWQKKRHFKRRFVIHERMQLVEQKLELGGTFHAATDWEPYAEWMLDVLDNRPALENLAGKGNSYPRPEWRPQTKFERRGIESGHKINDFIFKKIGS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.2. The protein design should consider the endogenous proteins and enzymes present in the N(7)-methylguanine-tRNA biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MPIGVPKVPFRSPGESDAAWFDLFNKLY",
    "response#2": "MCLGHTEFVSRIFVVPNHPELLLSSSGDCTLRLWEYRSGRELHC",
    "response#3": "MLLYC"
  },
  {
    "reference": "MSNIVLNTLRFKHPKLALLQSCSSFSDLKIIHGFLLRTHLISDVFVASRLLALCVDDSTFNKPTNLLGYAYGIFSQIQNPNLFVFNLLIRCFSTGAEPSKAFGFYTQMLKSRIWPDNITFPFLIKASSEMECVLVGEQTHSQIVRFGFQNDVYVENSLVHMYANCGFIAAAGRIFGQMGFRDVVSWTSMVAGYCKCGMVENAREMFDEMPHRNLFTWSIMINGYAKNNCFEKAIDLFEFMKREGVVANETVMVSVISSCAHLGALEFGERAYEYVVKSHMTVNLILGTALVDMFWRCGDIEKAIHVFEGLPETDSLSWSSIIKGLAVHGHAHKAMHYFSQMISLGFIPRDVTFTAVLSACSHGGLVEKGLEIYENMKKDHGIEPRLEHYGCIVDMLGRAGKLAEAENFILKMHVKPNAPILGALLGACKIYKNTEVAERVGNMLIKVKPEHSGYYVLLSNIYACAGQWDKIESLRDMMKEKLVKKPPGWSLIEIDGKINKFTMGDDQKHPEMGKIRRKWEEILGKIRLIGYKGNTGDAFFDVDEEEKESSIHMHSEKLAIAYGMMKTKPGTTIRIVKNLRVCEDCHTVTKLISEVYGRELIVRDRNRFHHFRNGVCSCRDYW",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be optimized for RNA binding activity in order to improve RNA modification. The designed protein sequence is ",
    "response#1": "KKFDTIVPIIPLIGSLAKAKFCNVSGHPISKPAWTDSSDSDIIDRFGRICRNLSHYYSGSSKKNSLYRVKYILRLSCARTLSRKHKSTVRSFLKRLGSKLLEEFLTEEEQVLSLIFPRVSSR",
    "response#2": "KKFDTIVPIIPLIGSLAKAKFCNVSGHPISKPAWTDSSDSDIIDRFGRICRNLSHYYSGSSKKNSLYRVKYILRLSCARTLSRKHKSTVRSFLKRLGSKLLEEFLTEEEQVLSLIFPRVSST",
    "response#3": "KKFDTIVPIIPLIGSLAKAKFCNVSGHPISKPAWTDSSDSDIIDRFGRICRNLSHYYSGSSKKNSLYRVKYILRLSCARTLSRKHKSTVRSFLKRLGSKLLEEFLTEEEQVLSLIFPRVSSR"
  },
  {
    "reference": "MQFEKVTYIAVPQKYGQKKVGVEEGPKFLEKLGFMNVLEQVAKSVNKKTITEPKTPQELGVTNARNLNEVESVNIELRDTIAKEYDVNNLLINIGGDHSIGLGTIAGVVKAMKPNARVGVVWFDAHPDMNTPENSPSGNIHGMPLACAVGLGPQRLTSIMPHYITPKDIMYVGIRSIDVGEQFEIQDKHIDHFTAEDVKRVGMKEVIEAINKKFVDYDVIHLSFDIDGIDPEFILGTGTPVPKGISLEDSLYFMSEMGKMKKLHSVDIVEYNPKIEEEITGKNVLKCISSLFGIKC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that can perform identical protein binding, arginase activity in cytosol to support urea cycle.2. The designed protein should be able to compete with endogenous proteins or inhibitors for Mn(2+) binding.3. The protein have enhanced H2O + L-arginine = L-ornithine + urea.4. The protein must exhibit the following characteristics: By depleting host L-arginine, a substrate for nitric oxide synthase (NOS), prevents the production of nitric oxide (NO) by host activated macrophages, and thus allows the parasite to evade host immune response.; Catalyzes the hydrolysis of L-arginine into urea and L- ornithine, which is a precursor for polyamine biosynthesis. The designed protein sequence is ",
    "response#1": "WSMDANLMHISYESGILENPKNHAPSDLYQMTKNPEHSPDQADMLEIEFKKGVPVRVSHLKDGISKETPLEIFCYLNEIGGKHGVGRIDIVENRFIGMKSRGIYETPGGTVLLQAHLDIETFTMDREVRKIKQSLGIKFSELIYNGFWFSPECEFVRECVERSQKHVEGRVQLSVYKGQVYILGRESPKSLYNEELVSMDVQGDYDPCDASGFIKINAVRLYSIDENLWGRSIEGDIISDPSLEVPEDAFEWTKQIYNKKEIVSIEFSNGVPTAVNGEKMELNKLVDFLNLKFGSHGFGRVEHIENRVVGFKSREVYEVPAALGLIYAHIDLEKTIYTPMELRFKRHIDQLWSDLVYQGLWFEPLRETLHKVADEMNKWISGEAKVEVSNGSFRIVGRESEYSPYSEKIASYNKGWYPSDEMARGFIEIWGMHR",
    "response#2": "WSMDANLMHISYESGILENPKNHAPSDLYQMTKNPEHSPDQADMLEIEFKKGVPVRVSHLKDGISKETPLEIFCYLNEIGGKHGVGRIDIVENRFIGMKSRGIYETPGGTVLLQAHLDIETFTMDREVRKIKQSLGIKFSELIYNGFWFSPECEFVRECVERSQKHVEGRVQLSVYKGQVYILGRESPKSLYNEELVSMDVQGDYDPCDASGFIKINAVRLYSIDENLWGRSIEGDIISDPSLEVPEDAFEWTKQIYNKKEIVSIEFSNGVPTAVNGEKMELNKLVDFLNLKFGSHGFGRVEHIENRVVGFKSREVYEVPAALGLIYAHIDLEKTIYTPMELRFKRHIDQLWSDLVYQGLWFEPLRETLHKVADEMNKWISGEAKVEVSNGSFRIVGRESEYSPYSEKIASYNKGWYPSDEMARGFIEIWGMHYSIDENLWGRSIEGDIISDPSLEVPEDAFEWTKQIYNKKEIVSIEFSNGVPTAVNGEKMELNKLVDFLNLKFGSHGFGRVEHIENRVVGFKSREVYEVPAALGLIYAHIDLEKTIYTPMELRFKRHIDQLWSDLVYQGLWFEPLRETLHKVADEMNKWISGEAKVEVSNGSFRIVGRESEYSPYSEKIASYNKGWYPSDEMARGFIEIWGMHYSIDENLWGRSIEGDIISDPSLEVPEDAFEWTKQIYNKKEIVSIEFSNGVPTAVNGEKMELNKLVDFLNLKFGSHGFGRVEHIENRVVGFKSREVYEVPAALGLIYAHIDLEKTIYTPMELRFKRHIDQLWSDLVYQGLWFEPLRETLHKVADEMNKWISGEAKVEVSNGSFRIVGRESEYSPYSEKIASYNKGWYPSDEMARGFIEIWGMH",
    "response#3": "WSMDANLMHISYESGILENPKNHAPSDLYQMTKNPEHSPDQADMLEIEFKKGVPVRVSHLKDGISKETPLEIFCYLNEIGGKHGVGRIDIVENRFIGMKSRGIYETPGGTVLLQAHLDIETFTMDREVRKIKQSLGIKFSELIYNGFWFSPECEFVRECVERSQKHVEGRVQLSVYKGQVYILGRESPKSLYNEELVSMDVQGDYDPCDASGFIKINAVRLYSIDENLWGRSIEGDIISDPSLEVPEDAFEWTKQIYNKKEIVSIEFSNGVPTAVNGEKMELNKLVDFLNLKFGSHGFGRVEHIENRVVGFKSREVYEVPAALGLIYAHIDLEKTIYTPMELRFKRHIDQLWSDLVYQGLWFEPLRETLHKVADEMNKWISGEAKVEVSNGSFRIVGRESEYSPYSEKIASYNKGWYPSDEMARGFIEIWGMHYSIDENLWGRSIEGDIISDPSLEVPEDAFEWTKQIYNKKEIVSIEFSNGVPTAVNGEKMELNKLVDFLNLKFGSHGFGRVEHIENRVVGFKSREVYEVPAALGLIYAHIDLEKTIYTPMELRFKRHIDQLWSDLVYQGLWFEPLRETLHKVADEMNKWISGEAKVEVSNGSFRIVGRESEYSPYSEKIASYNKGWYPSDEMARGFIEIWGMHPYKTYSIDENLWGRSIEGDIISDPSLEVPEDAFEWTKQIYNKKEIVSIEFSNGVPTAVNGEKMELNKLVDFLNLKFGSHGFGRVEHIENRVVGFKSREVYEVPAALGLIYAHIDLEKTIYTPMELRFKRHIDQLWSDLVYQGLWFEPLRETLHKVADEMNKWISGEAKVEVSNGSFRIVGRESEYSPYSEKIASYNKGWYPSDEMARGFIEIWGMH"
  },
  {
    "reference": "MSLASSLVVSSNVELSPSSFGFLDSVRDGPQIPDSFRFSSRNRVPNLINKKQKWGNHSHSTELKYPILHESGYGSVIVASMVANPAGEIAVSAEQKVYNVVMKQAALVKRQLRTAGELDVKPDIVLPGTLSLLNEAYDRCGEVCAEYAKTFYLGTMLMTPERQKAIWAIYVWCRRTDELVDGPNASHITPTALDRWEARLEELFQGRPFDMLDAALADTVTKFPVDIQPFKDMIEGMRMDLRKSRYKNFDELYLYCYYVAGTVGLMSVPVMGIAPESQASTESVYNAALALGIANQAPPNILRDVGEDARRGRIYLPQDELAQAGLSDEDIFAGRVTDKWRNFMKNQIKRARMFFDEAEKGVLELNKASRWPVWASLLLYRQILDEIEANDYDNFTKRAYVSKAKKILALPMAYGRALLGPS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that contributes to carotenoid biosynthetic process by functioning in plastoglobule with geranylgeranyl-diphosphate geranylgeranyltransferase activity, farnesyltranstransferase activity.2. A protein that can modulate the overall flux of metabolites through the Carotenoid biosynthesis, phytoene biosynthesis pathway, enhancing or suppressing its activity as needed.3. The protein have enhanced 2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2   diphosphate.4. For general function, the protein need meet that Catalyzes the reaction from prephytoene diphosphate to phytoene. The designed protein sequence is ",
    "response#1": "ELAVKLLEEKSRSFFVASAGFPSEVRERLVGLYAFCRVTDDLIDSPEVSSNPHATIDMVSDFLTLLFGPPLHPSQPDKILSSPLLPPSHPSRPTGMYPLPPPPSLSPAELVQFLTERVPVQYHFAFRLLAKLQGLIPRYPLDELLRGYTTDLIFPLSTEAVQARKTPIETTADLLDYGLCVAGSVAELLVYVSWASAPSQVPATIEEREAVLVASREMGTALQLVNIARDIKGDATEGRFYLPLSFFGLRDESKLAIPTDWTEPRPQDFDKLLSLSPSSTLPSSNASESFRFEWKTYSLPLVAYAEDLAKHSYKGIDRLPTEVQAGMRAACASYLLIGREIKVVWKGDVGERRTVAGWRRVLRAHSATFYLGSLLFPKEARKGAWAVYAACRLGDEAVDGEGGGPEALEAWWAGVERAYRGRPLAEWEKGLAWALERWDIPFEAFLHMREGFLTDLGPVRLGTEAELLRYCYQVAGTVGRMMAPIAGGGKEAEARAVKLGQAMQLTNILRDVGEDLERDRVYLPLDLLRAHGVEVEDLRAGRVTPGYRALMAHLEGKARALYREGLAGLGHLKVGRAAIALAALQYRGILDKLRLSGYDNLGRRAHLKAWERVLRAHSATFYLGSLLFPKEARKGAWAVYAACRLGDEAVDGEGGGPEALEAWWAGVERAYRGRPLAEWEKGLAWALERWDIPFEAFLHMREGFLTDLGPVRLGTEAELLRYCYQVAGTVGRMMAPIAGGGKEAEARAVKLGQAMQLTNILRDVGEDLERDRVYLPLDLLRAHGVEVEDLRAGRVTPGYRALMAHLEGKARALYREGLAGLGHLKVGRAAIALAALQYRGILDKLRLSGYDNLGRRAHLKAWE",
    "response#2": "MEVGSKSFATASKLFDAKTRRSVLMLYAWCRHCDDVIDDQVLGFSNDTPSLQSAEQRLAQLEMKTRQPMRIQMHEPAFAAFQEVAMAHDILPAYAFDHLAGFAMDVHETRYQTLDDTLRYCYHVAGVVGLMMAQIMGVRDNATLDRACDLGLAFQLTNIARDIVEDAEAGRCYLPAAWLAEEGLTRENLADPQNRKALSRVARRLVETAEPYYRSASAGLPGLPLRSAWAIATAQQVYRKIGMKVVQAGSQAWEQRQSTSTPEE",
    "response#3": "MEVGSKSFATASKLFDAKTRRSVLMLYAWCRHCDDVIDDQVLGFSNDTPSLQSAEQRLAQLEMKTRQPMRIQMHEPAFAAFQEVAMAHDILPAYAFDHLAGFAMDVHETRYQTLDDTLRYCYHVAGVVGLMMAQIMGVRDNATLDRACDLGLAFQLTNIARDIVEDAEAGRCYLPAAWLAEEGLTRENLADPQNRKALSRVARRLVETAEPYYRSASAGLPGLPLRSAWAIATAQQVYRKIGMKVVQAGSQAWEQRQSTSTPEMEVGSKSFATASKLFDAKTRRSVLMLYAWCRHCDDVIDDQVLGFSNDTPSLQSAEQRLAQLEMKTRQPMRIQMHEPAFAAFQEVAMAHDILPAYAFDHLAGFAMDVHETRYQTLDDTLRYCYHVAGVVGLMMAQIMGVRDNATLDRACDLGLAFQLTNIARDIVEDAEAGRCYLPAAWLAEEGLTRENLADPQNRKALSRVARRLVETAEPYYRSASAGLPGLPLRSAWAIATAQQVYRKIGMKVVQAGSQAWEQRQSTSTPESS"
  },
  {
    "reference": "MASDGARKQFWKRSNSKVPGSIQHVYGAQHPPFDPLLHGTLLKSTPKVPTTPVKAKRVSTFQEFESNTSDAWDAGEDDDELLAMATESLNSEVVMETAHRVLRNHSQRQSQPSQKTTEPEPEPQPIAEPPVPPSGDLRLVKSVSESHTPCPSESTGDTVPLQRSQSLPHSATVTLSGTSDPHALADSALSKRETSRLDKFKQLLAGPNTDLEELRKLSWSGIPKPVRPMTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYSSRNDEVHQDTYRQIHIDIPRMSPEALILQPKVTEIFERILFIWAIRHPASGYVQGINDLVTPFFVVFICEYTDREDVDKVDVSSVPAEVLRNIEADTYWCMSKLLDGIQDNYTFAQPGIQMKVKMLEELVSRIDERVHRHLDGHEVRYLQFAFRWMNNLLMRELPLRCTIRLWDTYQSEPEGFSHFHLYVCAAFLVRWRREILEERDFQELLLFLQNLPTARWDDQDVSLLLAEAYRLKFAFADAPNHYKK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "NGQQPKPNGNLVVIDLGAVIRNFEDLVLLDINRVGAAIGNSLVQLTAQADVPQEVINIVAQGIAAHVAGAAARQYTRQTGNTLRRITAMDPSKIYARKPNTLVGLARGNADFVDAIHTSAYGLGTTTRAGDVDFYPNGPSVNMPGTDDIIEASLRATRYLAETVLPGNDRNFPAVAAESLQQYKNNNGNGRRAYMGIAADYDLEGDYILQVNAKSPC",
    "response#2": "NGQQPKPNGNLVVIDLGAVIRNFEDLVLLDINRVGAAIGNSLVQLTAQADVPQEVINIVAQGIAAHVAGAAARQYTRQTGNTLRRITAMDPSKIYARKPNTLVGLARGNADFVDAIHTSAYGLGTTTRAGDVDFYPNGPSVNMPGTDDIIEASLRATRYLAETVLPGNDRNFPAVAAESLQQYKNNNGNGRRAYMGIAADYDLEGDYILQVNAKSPC",
    "response#3": "NGQQPKPNGNLVVIDLGAVIRNFEDLVLLDINRVGAAIGNSLVQLTAQADVPQEVINIVAQGIAAHVAGAAARQYTRQTGNTLRRITAMDPSKIYARKPNTLVGLARGNADFVDAIHTSAYGLGTTTRAGDVDFYPNGPSVNMPGTDDIIEASLRATRYLAETVLPGNDRNFPAVAAESLQQYKNNNGNGRRAYMGIAADYDLEGDYILQVNAKSPC"
  },
  {
    "reference": "MIAKHDITGLILAGGLGTRMGQQDKGMLALHGQPLALHVLQRLAPQVGALAFNVNEHHEHYTGFGLPIWPDDLSNHCGPLAGLQSGLRRASTPYLLTAPCDSPLLPPDLASRLAAGLLAHEADLAIAVTEEADTDGGAPRRRRHPVFCLVKTSALPRLDACLASGERRVGAWHGDLKLAEVLFEDGTAFRNINTPEELAQLNADAMPVDMAQALLAASVTPVTEVESLPLARALGRILACDIVSPISVPAHDNSAMDGYALHGADLAGDVPLTLAVADTILAGRPGTVAIAAGQCARIMTGAVMPAGCDSVLPQELVLTASEQTITLNPDAIRPGDNRRFKGEDLMQGQPALLQGKRLGPAELGLLASLGIAEVAVRRRLRVAFFSTGDELRQPGEPLAAGSIYDSNRYTLLGMLERLGCEPLDLGSVRDQPAALEAALRGACAKADVIITSGGVSSGAADHTREILARLGKVAFWQINMRPGRPMAFGTIHADGHSALLLGLPGNPVAVMATFYFLARPTLLRMMGANAQDLPLLSVPAQAAIRKKRGRTEYQRGIVERDTNGRQQVRVTGAQGSGILSSMSQANCMIVLHAHQGHVAAGELVDIVLFHGLV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of MoaB/Mog domains is critical for the interaction of the designed protein with its intended targets.2. The Mg(2+), GTP binding site should be stable and able to withstand changes in environmental conditions.3. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.4. The designed protein should be involved in Mo-molybdopterin cofactor biosynthetic process by localizing to cytoplasm and exhibiting metal ion binding, molybdopterin molybdotransferase activity, GTP binding, molybdenum cofactor guanylyltransferase activity.5. The protein must exhibit the following characteristics: Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor..6. The protein should be designed to have high stability and activity towards adenylyl-molybdopterin + H(+) + molybdate = AMP + H2O + Mo-   molybdopterin.7. A protein that is capable of catalyzing reactions within the Cofactor biosynthesis, molybdopterin biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MTGLVLAGGRGTRMGTVDKGLQPFGASTMVAHVLERLQPQVASIAINANQNLDAYGAFGVPVWPDDTPGFAGPLAGLEAGLRRCATHYLLTAPCDSPFLPADLAARLLAGLEAAGADLALAVTEENGLRQPHPVFCLVKADKAEVLSSYLAAGGRRPRVAYFSTGDEILSLGEAPREGAVYDSNRYTVLGLLRRLGCQPLDLGHVRDDPAALEARFREAAAQADAIITSGGVSVGEADFTKAMMRQLGDVAFWRIAMRPGRPMAVGRLSXSKTADPQRQPVDGRYQXMTSEHPGAVLFGLPGNPVAVMVTFLAFVRPRVAYFSTGDEILSLGEAPREGAVYDSNRYTVLGLLRRLGCQPLDLGHVRDDPAALEARFREAAAQADAIITSGGVSVGEADFTKAMMRQLGDVAFWRIAMRPGRPMAVGRLSXSKTADPQRQPVDGRYQXMTSEHPGAVLFGLPGNPVAVMVTFLAFVRAYFSTGDEILSLGEAPREGAVYDSNRYTVLGLLRRLGCQPLDLGHVRDDPAALEARFREAAAQADAIITSGGVSVGEADFTKAMMRQLGDVAFWRIAMRPGRPMAVGRLSXSKTADPQRQPVDGRYQXMTSEHPGAVLFGLPGNPVAVMVTFLAFVRPALLEGWSAVQPRVALFSTGDELAPPGAPLGFGQIRDSNRPMLAALLREAGAEVVQGAHVRDERETLRERLAAAAREADLIVTSGGVSAGEADHLPGLVRELGRIHFWKVRIKPGMPVLFGEIGGALVFALPGNPVSCAATFLALVR",
    "response#2": "MIKGIILAGGQGRRMGREKALVPLSGVPLIARVLALAPQVEAVAISANGDPGRFGLGLPVLPDRPGESGLGPMAGIRAGLDWAAGIGAEALVSTATDTPFLPEDLVERLAAAAPAHAQSFGRDHYTAALWRVATVPRIDALFAADEPRVAYFSTGDEILSLGEAPREGAVYDSNRYTVLGLLRRLGCQPLDLGHVRDDPAALEARFREAAAQADAIITSGGVSVGEADFTKAMMRQLGDVAFWRIAMRPGRPMAVGRLSXSKTADPQRQPVDGRYQXMTSEHPGAVLFGLPGNPVAVMVTFLAFVRTAEQAPLRVAFFSTGDELRSIGEQLDAGCIYDSNRYTLLGMLTRLGCEVLDMGIVKDQPQALEAALRGACAAADVIITSGGVSSGAADFTREILARLGEVAFWQINMRPGRPMAFGRIASGGETALLLGLPGNPVAVMATFYFLARLPAEFSFSKRAGRREFLRGWLTDAGGRVAMHPSTSSAHVSSLAQAEGLIDLPETVTQVQKGDLVRFPLAYFSTGDEILSLGEAPREGAVYDSNRYTVLGLLRRLGCQPLDLGHVRDDPAALEARFREAAAQADAIITSGGVSVGEADFTKAMMRQLGDVAFWRIAMRPGRPMAVGRLSXSKTADPQRQPVDGRYQXMTSEHPGAVLFGLPGNPVAVMVTFLAFVRPALLAFFSTGDELRSLGEKLDQGCVYDSNRYTLFGMLTRLGCEVIDMGVVRDDPQALEAALRQAASQADAIITSGGVSEGAADYTRDIMALIGDVAFWKLAMRPGRPLAFGKVQTEQDSAWLFGLPGNPVAVMVSFYMFARPALL",
    "response#3": "LSMTEARTRILNRVQPLTAALKLPLRDALGQVLAEAVISPHNVPNYTNSAMDGYALRGVDLPTDTPREYRVIGKAFAGGAFAGECGAGECVRIMTGAAMPAGTDTVVMQEQVTAGADGCVRIATGQQARQNVRLAGEDIAQGSSVLPAGRRITPADLGVLASLFSTGDEILSLGEAPREGAVYDSNRYTVLGLLRRLGCQPLDLGHVRDDPAALEARFREAAAQADAIITSGGVSVGEADFTKAMMRQLGDVAFWRIAMRPGRPMAVGRLSXSKTADPQRQPVDGRYQXMTSEHPGAVLFGLPGNPVAVMVTFLAFVRPALPRVAYFSTGDEILSLGEAPREGAVYDSNRYTVLGLLRRLGCQPLDLGHVRDDPAALEARFREAAAQADAIITSGGVSVGEADFTKAMMRQLGDVAFWRIAMRPGRPMAVGRLSXSKTADPQRQPVDGRYQXMTSEHPGAVLFGLPGNPVAVMVTFLAFVRAFFSTGDELRSIGEPLDEGCVYDSNRYTIYGMLQRLGCDIIDMGVVRDDPQALEAALREACENADAIITSGGVSVGAADYTRQIMARLGDVTFWKIGMRPGRPLAFGRIASNGHSAFLFGLPGNPVAVMVSFYFFAREALLAYFSTGDEILSLGEAPREGAVYDSNRYTVLGLLRRLGCQPLDLGHVRDDPAALEARFREAAAQADAIITSGGVSVGEADFTKAMMRQLGDVAFWRIAMRPGRPMAVGRLSXSKTADPQRQPVDGRYQXMTSEHPGAVLFGLPGNPVAVMVTFLAFVRPALL"
  },
  {
    "reference": "MRVLAIDWGEKYVGLAISDPLRIIAQGLDVWEIKGEEDFVSRLKRLVEDYQVKEIVLGYPISLRGHENERTQKVRHIAELIESVIGIPVKFVDERLTTMEAEKVLLEGDIKRKKRKLLKNKQAAVIILQKYLDSSSLDT",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have nuclease activity to facilitate rRNA 5'-end processing. The designed protein sequence is ",
    "response#1": "MRKLGIDFGLSRIGFAISDESNSFSLALENFDYDGSYEKIIQKIQLFLDEYKIDKFIIGYPLNSRGEKTKTCLLIDEFIIHLEKNFDQKIKLINESYSSRKAEARLE",
    "response#2": "MRKLGIDFGLSRIGFAISDESNSFSLALENFDYDGSYEKIIQKIQLFLDEYKIDKFIIGYPLNSRGEKTKTCLLIDEFIIHLEKNFDQKIKLINESYSSRKAEIKAA",
    "response#3": "MRKLGIDFGLSRIGFAISDESNSFSLALENFDYDGSYEKIIQKIQLFLDEYKIDKFIIGYPLNSRGEKTKTCLLIDEFIIHLEKNFDQKIKLINESYSSRKAELIRQ"
  },
  {
    "reference": "MAALRNRVFVIGVGMTKFEKPGARDIDYPDMAKEAGQRALADAGIKYSAIQQACVGYVYGDSTCGQRAIYHSLGLSGIPIINVNNNCSTGSTALFMGRQLIQGGLADCVLALGFEKMERGSLSSKYMDRTNPMDKHMEVMINRYGLAAVPAAPQMFGNAGREHMEKYGTKPEHFAKVAWKNHKHSTNNPYSQFQDEYSLEQVIDSRKVFEFLTLLQCCPTSDGAGAAVLASESFVRRNGLEKKAVEIVAQEMVTDLSTTFEENSCMKMVGYDMTRLAAERCYDTAGVKPSDVDVIELHDCFSANELITYEALGLCPEGKAGELIDRGDNTYGGKWVINPSGGLISKGHPLGATGLAQCAELCWQLRAEAGPRQVPGAKLALQHNIGLGGAVVVTLYKMGFPQETSSRIAAVSTSASSDRSGFKAHTVFKEIEKKLQEEGEAYVKKIGGVFAFKVKDGPGGSDALWVVDVKNGKGSVSSDAGKKADCTIAMADADLLDMMTGQLNPQTAFFQGKLKITGNMGMAMKLQNLQLTPGKAKL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein have Thiolase C-terminal, Thiolase N-terminal, SCP2 domains with specific sequence motifs that are crucial for its activity.2. The designed protein should have acetyl-CoA C-acyltransferase activity, lipid binding, propionyl-CoA C2-trimethyltridecanoyltransferase activity, acetyl-CoA C-myristoyltransferase activity within mitochondrion to regulate lipid transport, positive regulation of intracellular cholesterol transport. The designed protein sequence is ",
    "response#1": "ALADAGIPYSAVQQACVGYVYGDSACGQRAIYHSLGLTGIPIVNVNNNCATGSTALFMARQIVEGGLADCVLALGFEKMSKGSVSPSFLNRTNPLDKHMEVVINKYGLSGGPLAPQIFGNAGKEHMEKYGTNPEYFAKIAWKNHNHSINNPYSQFQTEYTIDEVLHSRKVFDFLTVLQCCPTSNGAAAAILANEFVKKHGLEKKAISVQIQGNPSGGLIAKGHPLGATYDMTAFAAKRLYEKTGISPKQVDVVELHDCFAANEMITYEGLQLCGEGEAGKFIDSGDNTYGGRVVVNPSGGLIAKGHPLGATGLAQCAELVWQLRGEADARQVPRARIALQHNLGLGGAVVVTLYRLGFPEEARKTWGELPKEFKALEAAMQTDDDNLIEKVRGVYAFKVRNGPNGKEGYWIVNAKTGKGSVEFNGKAKPDVTFTMDDADVVDLISGKLNPQKAFFQGKIKIQGNMGMAMKLQDLFKEIEKKLEEEGEQFVKKIGGIFAFKVKDGPGGKEATWVVDVKNGKGSVLPNSDKKADCTITMADSDLLALMTGKMNPQSAFFQGKLKITGNMGLAMKLQNL",
    "response#2": "ILQQAMNEDEEGLVERHRGIYGLKVVKKSGETGYWVINTKVGKGQIVFNGKDKPDVTFIVKEEDVLELLSGKIPPQKAFFQGKVKIQGNMGVAMRLIELLG",
    "response#3": "ILQQAMNEDEEGLVERHRGIYGLKVVKKSGETGYWVINTKVGKGQIVFNGKDKPDVTFIVKEEDVLELLSGKIPPQKAFFQGKVKIQGNMGVAMRLIELLG"
  },
  {
    "reference": "MHNLLALWQQRTRRERCLLLGMAVVLLIGLVYYTLWQPWQNREAQWRQTLAREQASLQWMRQQTPLIRQLRNQKPPTAPEEPSTVIMREAARHGLTIVRLQPQGSRLSLTVQPADFQALMAWLDALGQAGMTTATLAVTAVAQQPGWVTVNTLVLERSDEK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a specific function within the type II protein secretion system complex. The designed protein sequence is ",
    "response#1": "NLMGNKEKADQQKAVSDIVALENALDMYKLDNNRYPTTEQGLDALVNKPTAAPEPRSYRDGGYIKRLPQDPWGNPYQMLSPGQFGKIDIFSMGLDGEAGTDDDIGNI",
    "response#2": "NLMGNKEKADQQKAVSDIVALENALDMYKLDNNRYPTTEQGLDALVNKPTAAPEPRSYRDGGYIKRLPQDPWGNPYQMLSPGQFGKIDIFSMGLDGEAGTDDDIGNE",
    "response#3": "NLMGNKEKADQQKAVSDIVALENALDMYKLDNNRYPTTEQGLDALVNKPTAAPEPRSYRDGGYIKRLPQDPWGNPYQMLSPGQFGKIDIFSMGLDGEAGTDDDIGNL"
  },
  {
    "reference": "MKKIVLASGNKGKLREFAQLFATLDIEVVPQSDFDVPEAEETGLTFIENAIIKARNAAKHTGLPAIADDSGIEVDALXGKPGIYSARFAGANASDEDNLNFLLTSLQDKXEAXRAARYQCLLVYMEHENDPTPLISQASWEGRILTEXVGDGGFGYDPIFWVDETQCSAAELXADQKHAISHRGKALRIFAEQFQKTK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The desired protein should be able to perform dITP + H2O = dIMP + diphosphate + H(+)2. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP3. The protein design must be able to perform dITP diphosphatase activity in order to contribute to purine nucleoside triphosphate catabolic process. The designed protein sequence is ",
    "response#1": "MHKRIILATHNEHKVKEIRQLLAGASDFEIVSLKDYPDVPDVKETGNTFEENAAIKAADGARFTGLPAIADDSGLAVDGLDGAPGIYSARFAGENATDAENNTKLIADLKGVPWEKRTAHFTCVIALASPDGHEFTAEGCVEGFIRDKPAGENGFGYDPIFFVPEEKCSSAQMSAEEKNAISHRGKALAALKEKLK",
    "response#2": "MKLVFATNNRHKLEEIRSLT",
    "response#3": "MRVMLATGNAGKLAELRAVLGAAGLDLEVLPQGTLGVPEAEEPHRTFVENALAKARHAAAVTGLPAIADDSGLCVDVLGGAPGIFSARYAGRHGDDAANNALLLAQLGDVRDEHRGAHYVCVVALVQPSGAVVRGEGLWHGEIARAPRGEGGFGYDPLFLVPERGCSAAELSAEEKNRLSHRGRALAQLVPLVREALGG"
  },
  {
    "reference": "MNAAALVTLTTVPDVLAWLQARGVAALTVDSREVARQAAEAAASATGVAFIAWPGAARDGRAFVAQALADGAAACLVEAEGAQAFAFVDARIAAVPQLKARLADIAHAFYREPSAQLAVVAVTGTNGKTSTSWWTAQALSHLGRPCGVIGTLGVGQPGGGDFEATGLTTPDPVTLHATFRRFVDGGLTAAAIEASSIGIVEKRLDATRIAVAQFTNFTQDHLDYHGHMQAYWQAKQGLFEWPGLQAAVINFDDAQGEELEPLARQRGLDVWTYGLAAPVRLLAHDIAVRADGMRFTVTERDAALAGVVGTAHVDAPLIGDFNVLNLLAVLGALRALGVPLDEAAAACAALSAVPGRMQQVTLPEAPGAGLPLAVVDYAHTPDALHKALGALRTLTRARGGRLWCVFGCGGNRDATKRPLMGAMAEQHADEVVVTSDNPRDEKPCYILSQVLAGMARQDGVAVIENRHEAISHALKQCAPEDVVLIAGKGHEATQEVAGTKTPFSDVAEALAALRERSAA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The ATP binding site should be located in a region of the protein that is accessible to the ligand.2. The protein's functional design necessitates the inclusion of a Meso-diaminopimelate recognition motif motif.3. The protein must bind to Mg(2+) in order to perform its enzymatic function.4. The ideal protein would be one that plays a critical role in cell cycle, cell wall organization, cell division through ATP binding, magnesium ion binding within cytoplasm.5. The protein contains novel Mur ligase central domains that confer a unique function or activity.6. The protein design should consider the endogenous proteins and enzymes present in the peptidoglycan biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "ITGTNGKTTTAFLLRDMLEYAGVPSGLMGTVKQVVGGVEEEVVRTTPEAIDLQASFRRMSEAGDEACVMEVSSHAMVMHRADAIDFDLAVFTNLTQDHLDFHADMEDYFQAKRLLFAAGPGAAVVNVDDEYGRRLATEFDCLTYSASGSAADYAAVDVGFDAGGAAFTVQSEHGELAVRTGLPGSFNVANALAALAHELGFKDEQIAAGIRHFAGAQGRMQPVAPDGDASLVPWVLVDYAHTPDALEKALQALQPVAKQRDGALWVVIGCGGDRDRSKRPLMAAAAEREAQHAVLTSDNPRTEDPEGILRDMAAGLSRPQDASLLVDRAQAIAHAVAQARPQDVVLVAGKGHETYQIIGTQKFDFDDRVELARALQAHRGE",
    "response#2": "ITGTQGKTSTTYLVDAARGSVRSGVIGSMGTRIDGVAVPSTLTTPEAPQMQALLALMRERGVEVVSSEVSSHAITMNRVAGLHFDVGVFLNLGHDHRDFHGTQEAYLLAKRELLTSAMSGVGLVNVDDRAGRRLHADPELHTQSYSVTGREADWRAVDVELGLHGSSFTVVGPEGQSARFTTPMVGEFSVSNIVAAVAVTGTNGKTTVSSLLYQLFKKAGYKVGLLSTVKIMVDEVEYKATHTTPDSLTINRYLSEMNAEGVEFCFMEVSSHGIHQFRTEGLHFEGGIFTNLSHDHLDYHSTFAEYRDVKKKFFDELSSRAFALVNADDKNGLVMLQNTKAKTYTYALKQYADYKTQILENQFGGLLLKVNDSEVWTRLIGNFNAYNVLAIYAVLHDLGYDKTLIAAKLGEFTGVKRGRMQCHTLKNGAQAFVDFAHKPDAFEKVLKNLRPHTNNLIVVFGCGGNRDKTKRPVMGSSAAKYADLIIVTDDNPRDEDRQTIANEIIAGIPKEKRSQTICILDRKKAIEKAANMATPNSIIALLGKGDEDYQEIAGQKVPFSDADHAALALAKK",
    "response#3": "ITGTNGKTTTTYLVEHMARVAGKRTGVIGTVGVRIGNAQEKTAHTTPESPDLQKLLARMRDERCDVVAMEVSSHALDLERTWGTTFAVTAFSNLTQDHLDYHHTFEAYFEAKARLFSKGYPARRVICIDDKWGNELLRRCSVAEDSIVTTGFDSSAQIHPVDVSYEPTHTSVTLDVRGSRYSFDYPLVGRFNVENVMCAFGVLLEMGYSKEEIYPVTCHTDHVGPNSTFVVIKGFKEDGLQYIQTAIEKGATKVVLEKSHKEKILDQVQDDKNQAPDDKKVDYIFVEDTRVALAALCAGVPAEEVETALENFTGVPGRMQPVAPDGDASLVPWVLVDYAHTPDALEKALQALQPVAKQRDGALWVVIGCGGDRDRSKRPLMAAAAEREAQHAVLTSDNPRTEDPEGILRDMAAGLSRPQDASLLVDRAQAIAHAVAQARPQDVVLVAGKGDETYQIVGDERLPFDDREVAREAIRG"
  },
  {
    "reference": "MAKISLKLDELIDGEALRREMTALTTATAGDGSGAAARAGVLQLLKARLAEGRKIAEAMLKEDGGGNACAERLSHLMDELIRALYDFAATHVYRVKNRSSAERMAVVAVGGYGRGTLAPGSDIDLLFLLPYKQTPWGEQTVEYMLYMLWDLGLKVGHATRNIDECLRLSRTDITIRTSILEARFLWGERKLYDELMLRFDHEVVRTTGPEYVQAKLAERDERHAKAGESRYLVEPNVKDGKGGLRDLQTLFWIGKYFYRVRTGEELVEKGVFTEAEYREFQKAEDFLWAVRCHMHFLTGKAEERLHFDIQREIAERLGYTTHPGLSAVERFMKHYFLVAKDVGDLTRIFCAALEEEQAKHVPGFNRIFLTFQRRKRKLAGTSDFIVDNHRINIADDQVFERDPVNLLRLFWFADKHGLEFHPDALKLLTRSLGLVNKSLRRDEEANRLFLDILTSDRNAELNLRRMNEAGLLGRLIPDFGKIVAMMQFSMYHHYTVDEHLIRCIGVLAEIERGDGEKVHPLSHSLMPGLKKSREALYVAVLLHDIAKGRPEDHSEAGARIARRICPHMGLSAADTETVAWLVENHLAMSMTAQTRDLNDRKTIEDFASIVQSVERLKLLLVLTVCDIRGVGPGVWNGWKGQLLRTLYYETELLLTGGFSEVSRAQRTAAARERLAEALADWPDKDRKRYVGLHYENYLLTVDLPDQLRHAEFVREADAAGKKLATMVKTHQFEAVTEITVLAQDHPRLLSVIAGACVGAGGNIVDAQI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be able to regulate regulation of nitrogen utilization.2. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.3. For general function, the protein need meet that Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level The designed protein sequence is ",
    "response#1": "LMATIMGAAPRLAAIITRRPHVFDGLLDPALLTELPDRVYLSARLSAFLEGDRAYEEVLDRLRIFASEQKFLIGVRLLAGSIDPARAGRAFSDLADLTIEAALQAVTAEFAARHGTIAGGVVSLLGMGKLGSRELTAGSDVDLILLYDHDADAEDSDGDKPLAPSHYYTRMTQRLISAVSAPTAEGVA",
    "response#2": "LMATIMGAAPRLAAIITRRPHVFDGLLDPALLTELPDRVYLSARLSAFLEGDRAYEEVLDRLRIFASEQKFLIGVRLLAGSIDPARAGRAFSDLADLTIEAALQAVTAEFAARHGTIAGGVVSLLGMGKLGSRELTAGSDVDLILLYDHDADAEDSDGDKPLAPSHYYTRMTQRLISAVSAPTAEGVA",
    "response#3": "LMATIMGAAPRLAAIITRRPHVFDGLLDPALLTELPDRVYLSARLSAFLEGDRAYEEVLDRLRIFASEQKFLIGVRLLAGSIDPARAGRAFSDLADLTIEAALQAVTAEFAARHGTIAGGVVSLLGMGKLGSRELTAGSDVDLILLYDHDADAEDSDGDKPLAPSHYYTRMTQRLISAVSAPTAEGVR"
  },
  {
    "reference": "MITLKNVVSILKNDQNFRDLIIGGEYFSSCHVACTFDKLSYDSRDVTDSTLFFAKGLNFKRDFLTDLAIPFYVSEIDYEVAIPALIVNNIKHAMSLIAMHFYNQPQNELKTLAFTGTKGKTTAAYFAKNILDTHNGGKTALFSTMDTTLDGKTFFKSALTTPESLDLFRMMREAVENGMTHLVMEVSSQAYKTARVYGLTFDVGVFLNISPDHIGPIEHATFEDYFYCKRQLLANSRYAVVNAEMAHFEFVKSELTTDHAFYGKGAEFPISKSEAFSFSALGQKFEIQLIGRFNQENAVAAALASIRLGARLADTQIGIAKTTVPGRMEVLTQANGAKVFVDYAHNADSLEKLISVVTPHQTGRVTVVIGTTGNKGESRRRGVGELLERLPEIDVILTADDPNFENPADIAQEIASFITREVRIIVDRETAIRTALYETSSASDAVIIAGKGVDAFQIVNGAKVAYAGDRKIAEKYL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein should be able to manipulate the activity of enzymes in the Cell wall biogenesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "VTGTNGKTSVSLLLESILHKAGYHPGVMGTIDIHFADRTLPAPVTTPEAPDLQRIMAEMRDAGVTHLVMEASSQALDLYRLNGCAVDTAVFTNLSRDHLDWHQTMDRYWASKKRLFTEYLRSGTGAGTPPSPGLIPGVVVNADSPYGRELAALFPGNCVTTGQSGEYLVYSKTSEFRAAGIHGCLATPSGPVRVDSPLIGRHNLENIRLAAGAV",
    "response#2": "LVVIGGSQGAVGLNRMVRGALPSLLEAGCRVVHLTGNNDSDVGELDHPNLVEQPFSHEMPGLLQHADLAISRAGAGSLSELAVCGTPAILVPFPQAADQHQEANAACAAALGAAVIVHQHAAEHRALGHALEQLMGPRLRGNAAASNR",
    "response#3": "DRRWVPLDAISPHLVAAVVASEDSRFCTHHGIDWDAVEAARDHNEAGGRLRGASTLSMQTAKNAFLWPDRTWLRKGAELGFTLLIEATWPKRRIAEVYLNLAEWGDGIFGAEAAARRHFGKPAADLSAQEAALLAAVLPNPRRWSPERPTAYIRNRAATIERRL"
  },
  {
    "reference": "NNLISFTMKRIVLFLILLTKAASANLISFTFKRFNETNLILQRDATVSSGKLRITKAAENGVPTAGSLGRAFYSTPIQIWDNTTGTVAAWATSFTFNLQAPNAASPADGLAFALVPVGSQPKDKGGFLGLFDSKNYASSNQTVAVEFDTFYNGGWDPTERHIGIDVNSIKSIKTTSWDFANGENAEVLITYDSSTNLLVASLVHPSQKTSFIVSERVDLTSVLPEWVSVGFSATTGLSKGYVETNEVLSWSFASKISINKEDEENKLLISNLEGKAINNLA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with carbohydrate binding functions is required to enable defense response to fungus, killing of cells of another organism.2. For general function, the protein need meet that D-galactose-binding lectin.3. The protein's architecture should predominantly feature strand-strand-strand-strand-strand-strand-strand-helix-turn-strand-strand-strand-strand-strand-turn. The designed protein sequence is ",
    "response#1": "KVKGCDFTTSESTIFSKGYSINEISNKSSNNQQDIVCTVKAHANDLIGFKCPSNYSVEPHDCFVSAFNLSGKNENLENKLKLTNIIMDHYNNTFYSRLPSLISDNWKFFCVCSKDNEKKLVFTVEASISSC",
    "response#2": "KVKGCDFTTSESTIFSKGYSINEISNKSSNNQQDIVCTVKAHANDLIGFKCPSNYSVEPHDCFVSAFNLSGKNENLENKLKLTNIIMDHYNNTFYSRLPSLISDNWKFFCVCSKDNEKKLVFTVEASISSC",
    "response#3": "KVKGCDFTTSESTIFSKGYSINEISNKSSNNQQDIVCTVKAHANDLIGFKCPSNYSVEPHDCFVSAFNLSGKNENLENKLKLTNIIMDHYNNTFYSRLPSLISDNWKFFCVCSKDNEKKLVFTVEASISSC"
  },
  {
    "reference": "MRLVMASANPHKVEEISNILVGCRIDERPKGLADIEENAETLEGNAHLKATAVCNFSGDAAVADDTGLEVDYLNGLPGVRSARFAGEGATDVDNLMKLLKALEGVPEDQRTARFRTVAMVVFPDKSVLSAQGVVEGFISRLPRGEKGFGYDSIFVPVEGDGRSFAEMASEEKQLISHRGRAFRQLALSLSAKF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be optimized for ITP diphosphatase activity, dITP diphosphatase activity.2. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.3. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "MTP",
    "response#2": "MIRA",
    "response#3": "MQRLP"
  },
  {
    "reference": "MRRIIFATKNKGKIVEINEIMKELNLQILSLEEAAIDIEIIENGSTFEENAIIKVKEIMKITNDIVMADDSGLEIDYLNGAPGVHSSRFEGENTPYDIKNNKILDMLKGVAEEERTARFVCVIAAGFPDGQIITTKGVIEGIIGYEIKGENGFGYDPIFYVPEYKMTTAQMSSQLKNQISHRAKALNKMKELLKIRLGEAI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a high affinity for substrate.2. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.3. The protein must have high specificity towards ITP diphosphatase activity, XTP diphosphatase activity, nucleotide binding. The designed protein sequence is ",
    "response#1": "GSSGH",
    "response#2": "MF",
    "response#3": "MIG"
  },
  {
    "reference": "MAADTVDNNCIDFVAMKFIDNTLYFITENDENLESDYFGKLEPKLSIIRNLNDQVLFIDQGNKPVFEDMTDADCEANASQTKLIINLYKDSFARGMAVTISVNCKKIATLSCENKIISFKDISPPDSIKEPESDIIFFQTSVPGHDTKMQFESSLYKGYFLACIKESDLFKLILKKNDEPGDKSILFTVQNKD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be optimized for extracellular space-specific folding and stability, while maintaining cytokine activity.2. The protein must exhibit the following characteristics: Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.. The designed protein sequence is ",
    "response#1": "IYPIKKREDRRRLALIICNTKFDHLPARNGAHYDIVGMKRLLQGLGYTVVDEKNLTARDMESVLRAFAARPEHKSSDSTFLVLMSHGILEGICGTAHKKKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGEKHGELWVRDSPASLALISSQSSENLEADSVCKIHEEKDFIAFCSSTPHNVSWRDRTRGSIFITELITCFQKYSCCCHLMEIFRKVQKSFEVPQAKAQMPTIERATLTRDFYLFN",
    "response#2": "IYPIKKREDRRRLALIICNTKFDHLPARNGAHYDIVGMKRLLQGLGYTVVDEKNLTARDMESVLRAFAARPEHKSSDSTFLVLMSHGILEGICGTAHKKKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGEKHGELWVRDSPASLALISSQSSENLEADSVCKIHEEKDFIAFCSSTPHNVSWRDRTRGSIFITELITCFQKYSCCCHLMEIFRKVQKSFEVPQAKAQMPTIERATLTRDFYLC",
    "response#3": "RRLALIICNTKFDHLPARNGAHYDIVGMKRLLQGLGYTVVDEKNLTARDMESVLRAFAARPEHKSSDSTFLVLMSHGILEGICGTAHKKKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGEKHGELWVRDSPASLALISSQSSENLEADSVCKIHEEKDFIAFCSSTPHNVSWRDRTRGSIFITELITCFQKYSCCCHLMEIFRKVQKSFEVPQAKAQMPTIERATLTRDFC"
  },
  {
    "reference": "MLPSIKDLSYAELCQWLAQQQQPAFRAKQIRDWLYKKHATDFNDMANLPLTLRQTLAENFRAFTLTPLKTQEASDETVKWLSQLGDGHTIETVLIRTPERNTVCISSQVGCAVRCIFCASGKQGLLRNLSAAEMLDQIILASRAGGRLVNNLVVMGMGEPLHNFEQLVAALKLLCAADGLGLGARHVTISTSGVPAAIRRLADLQTPWNLALSLHAVNDEIRAKIIPPAKRSPLEDIFTACEYYTQKTKRMITLEYVLLEERNCRPEDASRLADIAHRLHAKVNLIPCNNDSGPCQAPDAELCERFLKLLLSRKVHATLRRSKGKDIQAACGQLRSQTPTGKSARGVAAATPAPPENSRE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must be able to perform tRNA binding under specific conditions.2. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.3. The S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "STQVGCAMGCGFCATGMLGFRRNMTPGEMLGQVLVARQYLLDKGEALALRNLVFMGMGEPLLNYDNLVKTLEALHHPQGLDFSGRRITVSTAGVRRNLLELGRTGLCSLAVSLHAPTQEKRARIMPGAAKLPLSELMGILREYPLKPRERLTFEYLLLDGVNDADADR",
    "response#2": "FTGCVSSQIGCSLACKFCATGYIDRKRNLDFDEIYDEVVLLNRESEAKYGHKLTNLVFMGMGEPLLNYKNVLKSIERITSPDGLGMSPRRITVSTAGVAKAIRQLGDDKVKFKLALSLHAPNDAKRNEIMNINASNDLKSLVDALNYFYQQTKNEITLEYILFKDFNDSLKDAEELVKLYRQIPADLVNIIEYNPIDLADFEKPEEEKVDAFMS",
    "response#3": "GCGMACPFCATGQGGLQRNLTASEIVWQVHDAARRLAAGELPGGPGHINNVVFMGMGEPMANYKAVLAAVRQIHAPNPDGLGISARGLTVSTVGMVPRMRQLADEDLPVTLAVSLHAPDDELRDEIVPLNKHFNVDAVLDAAWDYAAVTRRRVSIEYILIKDINDQARL"
  },
  {
    "reference": "MDERPVLFFDSGIGGLTVLREVRSLIPEKQFIYVADDVGFPYGNWEENVLKNRILKIFTNLLTVYNPALCVIACNTVSTLMIADLRERFPHILFVGTVPAIKLAAEQTKSGFISVLATPGTVKRTYTHELINSFAGQCHVQLVGSEKLAGFAENYLRGQSVDLEELRNEILPCFVKKNGKHTDIIVLACTHYPFLLHFFREQALWHVQWIDPSKAIAKRTRSLLPQRIHHQTVKKYEDFALFTSQNITSSTKRLLKEFSLNITKGVDFKV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have a binding site that is specific to the target substrate ligand.2. A protein that is capable of catalyzing reactions within the peptidoglycan biosynthesis, Cell wall biogenesis pathway with high efficiency and specificity.3. A key design requirement for the protein is the optimization of the Proton donor/acceptor to achieve high catalytic efficiency for L-glutamate = D-glutamate.4. The protein design must be able to perform glutamate racemase activity in order to contribute to peptidoglycan biosynthetic process, regulation of cell shape, cell wall organization. The designed protein sequence is ",
    "response#1": "MKILGVNIDHVATLRQARGTSYPDPVEAAFIAERGGADGITVHLREDRRHIQDRDVHLLRETVQTRLNLEMAATEEMIGIALEVRPDACTLVPEKREEVTTEGGLDVVGLGPFAEAIGRFHDAGIRVSLFIDPDEAQIRAAEAVGASFVELHTGAYAEAQGEEELARELARIRTAARYAQDLGLRVNAGHGLNYANVREIAAIEEIEELNIGHAIVARAVFIGFKEAVREMKDLMVGAR",
    "response#2": "MFLDAMIFLISFIAVLFSNYFFN",
    "response#3": "MNSPDDDRRP"
  },
  {
    "reference": "MKTTTALVTGATAGFGLAICKKLIEAGYKVIGTGRRADRLAEIHSQLGNNFLPLAFDIRDEQATINALNTLPEGWQAVDLLVNNAGLALGLEPAHKADLQDWYQMIDTNIKGLVTITRLVLPNMVARNYGQIINLSSIAGTYPYAGSNVYGGTKAFVTQFSLNLRADLAGTKIRVSNVEPGLCGGTEFSNVRFHGDDERAAKVYENVQSVQPEDIANIVLWLHQQPEHVNINRIEVMPTAQSFAGMSVSKEK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.2. The designed protein should have a binding site that is specific to the target substrate ligand. The designed protein sequence is ",
    "response#1": "TTYSVKREAEMMMWLSDKLKVPDVIEYGVREHSEYLIMSELRGKHIDCFIDHPIKYIECLVNALHQLQAIDIRNCPFSSKIDVRLKELKYLLDNRIADIDVSNWEDTTEFDDPMTLYQWLCENQPQEELCLSHGDMSANFFVSHDGIYFYDLARCGVADKWLDIAFCVREIREYYPDQ",
    "response#2": "TTYSVKREAEMMMWLSDKLKVPDVIEYGVREHSEYLIMSELRGKHIDCFIDHPIKYIECLVNALHQLQAIDIRNCPFSSKIDVRLKELKYLLDNRIADIDVSNWEDTTEFDDPMTLYQWLCENQPQEELCLSHGDMSANFFVSHDGIYFYDLARCGVADKWLDIAFCVREIREYYPDC",
    "response#3": "RPIIALDLPSAEAALDFVGRFDGENLFVKVGMELFYAAGPSVVTSLKEAGHDVFCDLKLHDIPNTVKGAASSLSRLGADLLTVHAAGGRSMMEASLEGLGDAAETTRVIAITQLTSTSPEALKTEQLVDVPLVESVRNYAKLAQAAGLAGVVCSAHEAADIASVTGPNFLRVTPGIRPAGSAVGDQSRVATPGNAASMGSSAIVVGRPITKADDPVAAYHAL"
  },
  {
    "reference": "MISVKKMKALELNAEYFGVSRLQLMENAGRAVAEEVSARFDAGKSRVAVFCGLGGNGGDGFVAARHLLTKGFEITVVIAGNPKKISDAAALQNWNALESLRDYLTIHEVSDGSNIPRVKAEVIIDALLGIGLKGKVRPPVLQLIRTINRVKGFRLAVDVPSGIDADTGEILGEAVKANLTVTFHKIKTGMRNAHEYTGEIVVRDIGIPPQVESLAGPGDVVLTKRKRTREAHKGDFGRLLIVGGSEEFSGAPALAAMAALRTGVDLVYVAAPEKTAYSISAMSPNLITVKLEGEQLNPDNVSQIEKHVERCDAVIIGPGLGTSSETEKAVKTLIKRVNSLKRPVLLDADGLKAYSKAKHPFKAPAVLTPHAGEYRLLTGKTPPKEPNKKALEVQSTAARLNAVILLKGSVDVISDGEHVKLNYTGNPGMTVGGTGDVLSGVVGAFLAQRVDPFEAAVAGAFVNGAAGDFAVDKLGYHIVPTDLLEFIPKVLDNPMSHIEEHKTVGRKR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a binding site that is specific to the target AMP ligand.2. The designed protein must have at least one YjeF N-terminal domain.3. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.4. We need a protein that can perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate with high efficiency. The designed protein sequence is ",
    "response#1": "IGKGELKALSKIEEESHKGNNGRTLVIGGSKDYYGAPIFSALAASKVVDIMTVATVSPVVPAVKNYPELMIKEFGGDYFNKHHVEELAHISKNYDSIVLGCGMGLKSETREFLQKYLNKILSNTDSENFPTLIIDADAIKLLNPKDFESYDSNNFNNANGTNNIHNVIDNSNKELLLKLFTNEKVILTPHKGEFEYIKPILEDLSEIYKLNIVLKGKTDIVFNKNNIKLNMTGNAGMTVGGTGDILAGLIAGFATKNEPYLSACSGIFVNGLAGDYLYDKIGYNYNSKDIIKILPKILYKV",
    "response#2": "GGTIMTGATRLVARAARRSGVGLLTIVSDASVLPVYAADAPGTMTASISGLEALLNDCRHNAFVVGPGYGVGDSTRGLVLQLLHLCRSTVLDADALTSFSDDPSTLCFAVQGPTVLTPHEGEFTRLFPDIDGDKLARARAAAKRSGATVLIKGADTVIAAPDGRAAISAVDAPWLATAGSGDVLSGMIAGLMAQGMEMFDAACMGVWLHGRSGTDC",
    "response#3": "GGTIMTGATRLVARAARRSGVGLLTIVSDASVLPVYAADAPGTMTASISGLEALLNDCRHNAFVVGPGYGVGDSTRGLVLQLLHLCRSTVLDADALTSFSDDPSTLCFAVQGPTVLTPHEGEFTRLFPDIDGDKLARARAAAKRSGATVLIKGADTVIAAPDGRAAISAVDAPWLATAGSGDVLSGMIAGLMAQGMEMFDAACMGVWLHGRSGTDR"
  },
  {
    "reference": "MKNHHYLLLTPGPLTTTKTVKEVMLYDWCTWDTEYNELVQEIRKRLVSLATKEEEYYTTVFMQGSGTFSVESVIGSVIPQEGKLLVCTNGAYGKRIVQIATTLHINVVESYTNEWEPTNLAEVEEILQKDSEITHIAVVHCETTTGIINPIADVCRLGKQYGKVTIVDAMSSFGGVEMDVADLQIDFMISSANKCIQGVPGFGFVIAKRTELEQCKGRARSLSLDLYDQWETMELQNGKWRFTSPTHTVRAFYQALLELEEEGGVKARNTRYRNNQQVLVKRMREVGFEPLLDEAYQSPIITSFLYPGEGFTFQQLYEELKEHGFVIYPGKISKVDTFRIGNIGDVYESDIHQLVDCISEGVVIG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be able to modulate organic phosphonate catabolic process in a way that leads to a desirable outcome.2. The protein should be designed to have high stability and activity towards (2-aminoethyl)phosphonate + pyruvate = L-alanine +   phosphonoacetaldehyde.3. For general function, the protein need meet that Involved in phosphonate degradation. The designed protein sequence is ",
    "response#1": "ISDAIEDGSIQQNQALLKLCAEIRPKKSKLHLIGLCSEGGVHSHLDHLLGLLDLAKVQGISDVCIHVITDGRDTNVTDGMPAIKRIQEHINKIKLGRMVTLSGRYYAMDRDRRWDRVEKAYNVMTSDQGIDGRSITEVLQAFYDQNITDEFIPPTRIAPGAIEAGDGVIFYNFRPDRARQLCYALTMPDFEDFDRDLISPLSFVTFTQYR",
    "response#2": "MQGSGTFSVEAAIANVVPRDGKVLVPQNGAYCQRILKICKVLGRAHVELPIPEDRPATAAAIEAALKKDPSITHVAQVHCETGAGVLNPLPEIAAVCARLGKGLIVDAMSSFGAIEIDARTMPFDALVAATGKCIEGVPGMGFVLVKKTVLEGSQGNSHSLALDLYDQYTYMQKTTQWRFTPPTHVVAAFRTALDQFLEEGGQPVRGERYRRNYETLVQGMAVLGFRPFLSPDVQAPIIVTFK",
    "response#3": "MQGSGTFSVEAAIANVVPRDGKVLVPQNGAYCQRILKICKVLGRAHVELPIPEDRPATAAAIEAALKKDPSITHVAQVHCETGAGVLNPLPEIAAVCARLGKGLIVDAMSSFGAIEIDARTMPFDALVAATGKCIEGVPGMGFVLVKKTVLEGSQGNSHSLALDLYDQYTYMQKTTQWRFTPPTHVVAAFRTALDQFLEEGGQPVRGERYRRNYETLVQGMAVLGFRPFLSPDVQAPIIVTFR"
  },
  {
    "reference": "MNYQNSLAFAIERDQQDPLKDFRKQFLIPQHNGKDVVYLCGNSLGLQPSSASKYINDQLSNWQNLAVEGWFQGDDPWLEYHKSLAISVSKIVGAKESEVTVMNSLTVNLHLLMVSFYKPNSKRYKILMEGGAFPSDQYAVESQVRFHGFDPKDAVIEIFPREGEVTLRTEDIIQQIRDNADEVALVLFAGINYYTGQFFDLQAITKAGHDAGAYVGFDLAHAAGNVPLQLHDWGGDFACWCSYKYMNSGPGGISGVFVHEKHFNNQQLDRFAGWWGYRADKRFLMAPGFEAANGANGWQVSTAPILLQALFKASMAIFDSAAGICRLREKSLALTGYLEFLIGQINKQQGEEVFRIITPANPHERGCQLSVVCLRNAKAIFQYLANNGVIGDWREPDVIRLSPVPLYNTFEDVYHTGKQLLAAIAQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a fast turnover rate for H2O + L-kynurenine = anthranilate + H(+) + L-alanine.2. The protein must exhibit the following characteristics: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively..3. The protein must be engineered to have a cooperative binding mechanism with pyridoxal 5'-phosphate to ensure proper regulation.4. The protein design should take into account the physiological conditions of the Cofactor biosynthesis, quinolinate from L-kynurenine: step 2/3 pathway.5. The pyridoxal 5'-phosphate binding site should be stable and able to withstand changes in environmental conditions.6. The protein contains novel Aminotransferase class V domains that confer a unique function or activity.7. The protein design should be able to enhance the efficiency of tryptophan catabolic process, anthranilate metabolic process. The designed protein sequence is ",
    "response#1": "MSATKQQSAKIVGALPSEVVVMETLTANLHLLMSSFYRPTADRWKIIIEGKAFPSDHYAALSQIAHHNLDPSALITIEPPSASSPYLSTEHILSVISKHAATTALVLLPGIQFYSGQFLDIELITRHCHSNGIPIGWDLAHAVGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHEGDKLYQSATKQQSAKIVGALPSEVVVMETLTANLHLLMSSFYRPTADRWKIIIEGKAFPSDHYAALSQIAHHNLDPSALITIEPPSASSPYLSTEHILSVISKHAATTALVLLPGIQFYSGQFLDIELITRHCHSNGIPIGWDLAHAVGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHEGDKLYQSATKQQSAKIVGALPSEVVVMETLTANLHLLMSSFYRPTADRWKIIIEGKAFPSDHYAALSQIAHHNLDPSALITIEPPSASSPYLSTEHILSVISKHAATTALVLLPGIQFYSGQFLDIELITRHCHSNGIPIGWDLAHAVGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHEGEKLYQKS",
    "response#2": "DQAKEAMSKIVGALPSEVAVMQTLTANLHLLMASFYRPTKNRWKIIIEGKAFPSDHYAVYSQLAHHNLSPTDALVLIEPSNPETHNFSTQHILDTISAHAETTALLLLPGIHYYTGQYLDIKTITSFAQSKGITVGWDLAHAAGNVPVQLHDWNVDFAAWCTYKYLNAGPGSIAGLFG",
    "response#3": "DQAKEAMSKIVGALPSEVAVMQTLTANLHLLMASFYRPTKNRWKIIIEGKAFPSDHYAVYSQLAHHNLSPTDALVLIEPSNPETHNFSTQHILDTISAHAETTALLLLPGIHYYTGQYLDIKTITSFAQSKGITVGWDLAHAAGNVPVQLHDWNVDFAAWCTYKYLNAGPGSIAGLFGQDRAIEEATKQQSAKIVGALPSEVVVMETLTANLHLLMSSFYRPTADRWKIIIEGKAFPSDHYAALSQIAHHNLDPSALITIEPPSASSPYLSTEHILSVISKHAATTALVLLPGIQFYSGQFLDIELITRHCHSNGIPIGWDLAHAVGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHEGA"
  },
  {
    "reference": "MRVKGIRRNCQPWWIWGILGFWMFMICNGTGNLWVTVYYGVPVWREAKTTLFCASDAKAYSKEVHNVWATHACVPTDPSPQEIVLGNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCSNNITHNGTTYYNNGTTYSNGTISYNKSIIDEIKNCSFNVTTEIIDRKKQEYAIFYKIDIVSLNETSRTSNESKTFSMYRLINCNTSTITQSCPKVSFDPIPIHYCAPAGYAILKCNNKTFNGTGPCTNISTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRTVNMTDNAKTIIVHLNESVEITCTRPNNNTRKSMRIGPGQTFYATGEIIGNIRQAHCNISESKWNTTLYKISEKLREYFPNKTIIFANSSGGDIEITTHTFNCRGEFFYCDTSKLFNGTYSESNKTENTSSVITLPCRIKQIVNMWQEVGRAMYAPPIAGNITCRSNITGLLLTRDGGNVNSTNETFRPEGGNMRDNWRSELYKYKVVEIKPLGIAPTKAKRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKSEKDIWDNMTWMQWDREINNYTNTIYRLLEESQSQQEQNEKDLLALDSWKNLWNWFSISNWLWYIKIFIMIVGGLIVLRIIFAVLSIVNRVRQGYSPLSFQTLIPNPTGPDRLGRIEEEGGEQDKDRSIRLVSGFLALIWD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Include a signal peptide as an essential component of the protein.2. The designed protein should contain one or more Retroviral envelope protein GP41-like domains that are essential for its function.3. The protein should be optimized for stability and expression in viral envelope, host cell endosome membrane, virion membrane where it will participate in positive regulation of receptor clustering, actin filament reorganization, viral protein processing, fusion of virus membrane with host endosome membrane.4. The protein must exhibit the following characteristics: The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane.5. The protein should have Helical domains to ensure proper insertion into the membrane.6. The designed protein should contain at least one YXXL motif; contains endocytosis signal motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "MWVTVYYGVPVWREAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQELVLENVTENFNMWKNDMVDQMHEDVISLWDESLKPCVKLTPLCVTLNCSTILNCSTLNCSTTQSGNDTCNNNMCAEMKNCSFNTTTELRDKNRKEYALFYKLDIVPLERANNRTENNSSEEYRLINCNTSTTTQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGKGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENLTNNAKTIIVHLNESVEIVCTRPNNNTRKSIRIGPGQTFYATGAIIGDIRQAHCNISKSKWNSTLQRVGKKLAEHFPNRTIRFNSSSGGDLEITTHSFNCGGEFFYCNTSGLFNSTFNDTYAPNNTDTNETITIQCRIKQIINMWQQVGRAMYAPPIAGNITCNSSITGLLLVRDGGTKTDDNTTETFRPEGGNMRDNWRSELYKYKVVEIKPLGIAPTEAKRRVVQREKRXLGAAMVGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLDIIWGNMTWMEWDREINNYTSLIYNLIEQSQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLRIVFTVLSIVNRVRQGYSPLSFQTRLPAQRGPDGPEEIEEGGGERDRDRSGRLVDGFLALIWVDLRSLCLFS",
    "response#2": "MWVTVYYGVPVWREAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQELVLENVTENFNMWKNDMVDQMHEDVISLWDESLKPCVKLTPLCVTLNCSTILNCSTLNCSTTQSGNDTCNNNMCAEMKNCSFNTTTELRDKNRKEYALFYKLDIVPLERANNRTENNSSEEYRLINCNTSTTTQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGKGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENLTNNAKTIIVHLNESVEIVCTRPNNNTRKSIRIGPGQTFYATGAIIGDIRQAHCNISKSKWNSTLQRVGKKLAEHFPNRTIRFNSSSGGDLEITTHSFNCGGEFFYCNTSGLFNSTFNDTYAPNNTDTNETITIQCRIKQIINMWQQVGRAMYAPPIAGNITCNSSITGLLLVRDGGTKTDDNTTETFRPEGGNMRDNWRSELYKYKVVEIKPLGIAPTEAKRRVVQREKRXXXXXXXLWVTVYYGVPVWRDANTTLFCASDAKAYDTEAHNVWATHACVPTDPNPQEIKLTNVTEYFNVWKNNMVEQMNEDVINLWDQSLKPCVRLTPLCVTLNCTNVTVNITDTSANNVTNSTIGIEIMKNCSFHINTDRGKKVRKEYALFYSLDVIKDDDDKNNTKYRLVSCNTSAVTQACPKITFEPIPIHYCAPAGFALLKCNDKKFNGSGTCANVSTVQCTHGIRPVVSTQLLLNGSLAEGEVVIRSENFSDNAKTIIVQLNESVVINCIRPNNNTRKSINMGPGRAFYATGDIIGDIRQAHCNLSRAQWNNTLRQIVGKLREQFGNNKTMIFHQSAGGDPEIVMHSFNCGGEFFYCDTASLFNSTWQAKETERNDTGGNDTITLPCRIKQIINMWQAVGKAIYAPPIAGLIRCSSNITGLLLTRDGGNETMTKDNETFRPGEGNMRDNWRSELYKYKVVKVEPLGIAPTKARRRVVQREK",
    "response#3": "MWVTVYYGVPVWREAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQELVLENVTENFNMWKNDMVDQMHEDVISLWDESLKPCVKLTPLCVTLNCSTILNCSTLNCSTTQSGNDTCNNNMCAEMKNCSFNTTTELRDKNRKEYALFYKLDIVPLERANNRTENNSSEEYRLINCNTSTTTQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGKGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENLTNNAKTIIVHLNESVEIVCTRPNNNTRKSIRIGPGQTFYATGAIIGDIRQAHCNISKSKWNSTLQRVGKKLAEHFPNRTIRFNSSSGGDLEITTHSFNCGGEFFYCNTSGLFNSTFNDTYAPNNTDTNETITIQCRIKQIINMWQQVGRAMYAPPIAGNITCNSSITGLLLVRDGGTKTDDNTTETFRPEGGNMRDNWRSELYKYKVVEIKPLGIAPTEAKRRVVQREKLVLPGELAKHAVSEGTKAVTKWVTVYYGVPVWKDAETTLFCASDAKAYNTEAHNVWATHACVPTDPNPQEIQLENVTEKFNMWKNNMVEQMHTDIISLWDQSLKPCVQLTPLCVTLNCSNINSTSSNSTSGNSTVSNKEELKNCSFNVTTEIRDKKKKEYSLFYKLDVVPISSTNSNSSSGEYRLINCNTSTITQACPKVTLEPIPIHYCAPAGFAILKCKDENFTGIGLCNDVSTVQCTHGIRPVVSTQLLLNGSLARERVKIRSENITDNGKNIIVQLVDPVNITCTRPSNNTRKSIHIGPGQAFYATGGIIGDIRQAHCNISELAWNKTLQGVVEQLRGHFKNKTIIFASSSGGDLEITTHSFNCAGEFFYCNTSKLFNSTFPLNNTQNNSTQTNSTITLPCRIKQIINMWQRTGKAIYAPPISGPIKCTSNITGLILTRDGGNDNNNDTNGNETFRPGGGDMRDNWRSELYKYKVVKLEPLGVAPTRARRRVVQREK"
  },
  {
    "reference": "MTEILTSAQMRAIESAAIESGQVSGAELMERAGKGVIEALFARWPDLAAGSHRAVVLCGPGNNGGDGFVIARLLKHWGWDVRVHLFGAAERLPPDARLNHDRWAALGPVAPLPSVPDMRGAVVIDALFGTGLTRPLPAALLSVADGAARVVAVDIPSGLCADSGRAIGGTALRADLTVSFHRLKLGHVLADGPVHCGEVVVCPIGLRGGVGVGLAQPDTVLLSKRGGHKYAHGHALVLSGGAGCTGAARLAARAALRVGAGLVTLGVPAEAGPEVAAQITAVMLRRVEGGADLAAVLEDARYSALCLGPGLGLGARARGLVRSALNGVRPVVLDADALSLFASGPETLFNLIHGAEVVLTPHDGEFARLFPDLAAPLHAPAGTGPAASRLVAARQAARRAGAVVLLKGPDTIIAAPDGTAVVSAASYERAAPWLATAGAGDVLAGLICGLLARGAPPFAASRTAAWLHVEAARVFGPGLIAEDLPEILPRVLRRLQV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have ATP binding, NADHX epimerase activity, kinase activity, metal ion binding, ADP-dependent NAD(P)H-hydrate dehydratase activity to facilitate nicotinamide nucleotide metabolic process, phosphorylation.2. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.3. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.4. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.5. The protein should be able to bind (6S)-NADPHX, AMP, K(+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MRVTDKNTQYNHIPTIVLMENAGSRIADYLINNYPDKKKVSIYSGTGGNGGDGFVIARHLLNHGYKVRLFMLSKPENIKNKDSQLNYQSIKIISQTDKNLKLSTITDSSQVSPDNSDIIIDAILGTGVNGKLRQPVSSVIDTINYSPAIVISVDVPSGMNPENGKVTDKCVVAHETLTLHKMKTGLQKAPESYVGKISVLDIGIPRVSEECRYIAGSGDVLTGIGSVLIIGSNPDYIGAVVFAAQAALSQRMDLVYIIAPEASSNIIKQYNPEFIVRGVPGDTLTMDAYDAIMQLEKRVDSILIGSGSGTDEKTGELFNNILSSTEKPLVIDADALKLISPEMLSQNMVVTPHAHEFKQLFGMEVPEKIDERVEMLMNLSGDYGCQILLKGVTDVITSPEDYKLNRTGNQGMTKGGTGDLLAGLTLALSTKNTLFESAFLSAFILGSACDKLLEEKGLDYGIRDILKE",
    "response#2": "MRVTDKNTQYNHIPTIVLMENAGSRIADYLINNYPDKKKVSIYSGTGGNGGDGFVIARHLLNHGYKVRLFMLSKPENIKNKDSQLNYQSIKIISQTDKNLKLSTITDSSQVSPDNSDIIIDAILGTGVNGKLRQPVSSVIDTINYSPAIVISVDVPSGMNPENGKVTDKCVVAHETLTLHKMKTGLQKAPESYVGKISVLDIGIPRVSELALGGSPGFTGAITLTARAALRAGAGLVTAAVPRGCQPVVAAGAPEIMTWGLPETAGGSLGRSALDPLLERLENFSVLAIGPGLGRDPETVELVKSLLPRASIPLVVDADALNALATDTGILSQPHGPMVLTPHPGEMGRLIGCPAAKIQEDRLNIARRYAQEWRCVLLLKGARTVVACPDGRAYINPTGNPGMATGGSGDVLTGIIAGLIAQGMSLDKAAAIGAYLHGAAGDLAAEELGQEALIAGDILNAHKRTYGHLGVLVGEKPGAGIMAAMAAFNFGAGLVSVVSHDDKINIPYEIMHSHHIQGFSALAFGMGLDNHFDDEMEEILKLDIPMVIDADMFYRSEIRDFLSKENIVLTPHPKEFAALLKVVGMGEYSPSDVQKNRFYLAEEFSKKYPKIVLLLKGANKIIAYKGNLHIDPNGSVALAKGGSGDVLSGMIGSLLAQGWHPLKATIHASLAHSIAGNYEPNYALTPLKLIN",
    "response#3": "MRVTDKNTQYNHIPTIVLMENAGSRIADYLINNYPDKKKVSIYSGTGGNGGDGFVIARHLLNHGYKVRLFMLSKPENIKNKDSQLNYQSIKIISQTDKNLKLSTITDSSQVSPDNSDIIIDAILGTGVNGKLRQPVSSVIDTINYSPAIVISVDVPSGMNPENGKVTDKCVVAHETLTLHKMKTGLQKAPESYVGKISVLDIGIPRVSEVLVAGGERGFSGAVQMAASAALRVGAGLVSLATRSEHAALLNLVRPELMVHGIDKRVDLAPLLKKSSVAAVGPGLGQTVWAQNLLAELFDTAMPLVVDADALNLLAKEPFKRDNWVLTPHPGEAARLLGITIKEIESDRFSSVKALQKKYGGVVVLKGAGTLIFDGSEMVINTTGNPGMASGGMGDVLSGVIAALLAQGLSLSQSARLGVWLHGKAGDIAAKEGERGLLASDLLPESDKYRRGVVGVAAGSERYPGAAVLAVAGALRGGAGAVRYVGPPAEAVLARFPEVLIGPGRVQAWVAGSGLGDAPEARERLARALDDGVSTVVDADGLRMLGPAPGRTARGPLVLTPHAGEAAALLGVAREEVEEARLESVRQLARQTGATVLLKGSTTLIASPPGEADAGEEDGGTVLANLNGTGWLATAGTGDVLAGLIGSLLAAGLAPADAAAVGAHLHGLARFTADGTCLPKREKTAHKGLFGHVLVAGGERGFSGAVQMAASAALRVGAGLVSLATRSEHAALLNLVRPELMVHGIDKRVDLAPLLKKSSVAAVGPGLGQTVWAQNLLAELFDTAMPLVVDADALNLLAKEPFKRDNWVLTPHPGEAARLLGITIKEIESDRFSSVKALQKKYGGVVVLKGAGTLIFDGSEMVINTTGNPGMASGGMGDVLSGVIAALLAQGLSLSQSARLGVWLHGKAGDIAAKEGERGLLASDLLRPLRVLVN"
  },
  {
    "reference": "MVSDENLLSARPRRPVATPLADLAAWLEGEDADTTTSGDLAAAPDVTGLSLSSQRILPGDVYAALPGTRVHGIEYAATAVGAGAVAILTDPDGAAAVDAPGVPLLVVARPRAVLGRLAARVYGNPATALRMIGVTGTQGKTTTTRLAEGGLQQAGIPAAVIGTVGTRVAGTDLRTTLTTPEAPDLHGLFAMMRERRVAVCAMEVSSHALVMGRVDGVVFDVAVFLNLGRDHLDFHADVEDYYRAKASLFTPERARLGLVNVDDEHGRRLAAEATIPIRTFSLTGRDAHWQAVDVELEAGGSRFTVLGPDGIAVDAAVPLPGDFNVGNALAAIAACAEVGVDTARVADGIAAAGGVPGRLERVDAGQDFVVVVDYAHKPDAVEAALRTLRPLTDGAVIVVIGAGGDRDPGKRPIMGEIASRLADVLVVTDDNPRTEDPAAIRAAVLAGATGGRAEVLEIGDRRAAIREAVRRARPGDIVVVAGKGHETGQTVAGVVHPFDDREVVRAELRSR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should include a Meso-diaminopimelate recognition motif motif to ensure proper function.2. The protein should be designed to catalyze the following reaction ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.3. The presence of Mur ligase N-terminal catalytic, Mur ligase central domains is critical for the interaction of the designed protein with its intended targets.4. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..5. A protein that is capable of catalyzing reactions within the peptidoglycan biosynthesis, Cell wall biogenesis pathway with high efficiency and specificity.6. The protein should be able to bind UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP, meso-2,6-diaminoheptanedioate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MKLRDLVPQVLDGVPSPVSGLAQDSRRVRPGDLFLAVNGARGHGLAHWPEALRRGAVGVLWDPQEPAIAGLTHDSRAVNPGWLFVCLIGQNFDGHQFAPQAVERGAAAVVVQQDALEKLGITLPPHIPIVKVPDTRKAFPLLACAFHGHPSRRLQLVGVTGTNGKTSVTSFISQLLDAAGLPCGVLGTLGYGMPGELREATHTTPDVVEVNRALFELARKGARAVAMEVSSHALDQGRVQGVAMIGAVFTNLTRDHLDYHGTMEAYGQAKASLFTREGLHFSVINFDDPFGRQLFEQTEGMCDRVRYSLHESQTELWLTRFAPTPDGFEADVDGQWGLFNLSAPLLGTFNASNLLAAVAAIAGLTADSREVRPGWLFAALAGSKADGARFIDDALAKGATAVLVKEGTAVSAPAEVAVLSATEPRRALALMAARFGADPERVAAGLRTLTGVAGRMEPFRAPGRPLAVVDYAHTPAALEQALRALRAHVSGRIFCVFGCGGSRDRGKRPLMGAVAERLADRVILADDNPRDERPEAIVREILAGTRHPEAVQVIHDRAQAIATALELAGPGDAVLVAGKGHETYQVVGDVTVPFDDRIVARDALAARRP",
    "response#2": "MKLKDLFQNILLKQVKGNLDVEISEIVFDSRKAVEDSMYVALKGSVSDGHSFIAAAIEKGAKAIVCEDFPANLEAKIIYIKVKDASVTLGNLAANFYNNPSTKLKLIGVTGTNGKTTTTHMIANILRTAGYKVGLIGTIETIIGDKKLPATHTTPESTIIQKYFKKMVDAGCDSVVMEVSSQGLMLHRTRGFTFDYGIFTNLTQDHLDFHKTFESYFNAKKKLFTEYNPKTGIINIDDEYGKRIIKESDANVITFSIDNEADVKASNIKYSSSGTDFYLNTYLGEVKINTKLIGKFNVYNILAAITASLVVGVSLKDIITSLEKVKGVSGRMEMFRVTHKPEVVIDYAHTPCALEQALIETRRYCKGKLWCIFGCGGDRDSGKRPMMGKLAERLSDFLIVTNDNPRSEDPVAIIRDILSGLVYPQTAYVIINRVQAITYAIKQAKINDVVLIAGKGHETYQILKDKTIHFDDREIAKEIISLLQ",
    "response#3": "MSIAGLTADSREVRPGWLFAALAGSKADGARFIDDALAKGATAVLVKEGTAVSAPAEVAVLSATEPRRALALMAARFYGRQPRQMVAITGTNGKTSTAWWLAEALSRSERIAQSPCGVIGTLGIGMPPALVSTGLTTPDPVMLQRELRAFVSRGFGACAIEASSIGIAERRLDGTRIAVAVFTNFTQDHLDYHGDMDAYWQAKAELFRWPGLRAAVVNLDDVHGASLVGNLVESGVGSLDVWTVSAAGSPARLRATDIGYGVDGLEFSIVESSLPVERLATQLIGQYNVDNLLGVVAMQSLGFSLEQISAALANMDGVPGRFEPVPTAHRGFSVIVDYAHTPDGLQNLLQSALRLQPKRLICVFGCGGNRDRGKRPIMGNLAATMADIAIVTSDNPRHEDPNAIIQEILTGMNPERDPHIKAQIIVEPDRRAAIYKAVALAQPGDMVLIAGKGHETYQIIGNERLPFDEREIARRAIAERGGAA"
  },
  {
    "reference": "MRVTGIRKNYPPLWIWGXMLLGMLMICKGEDKLWVTVYYGVPVWKEATTTLFCASDAKGYNTEVHNVWATHACVPTDPSPQEMVLENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDYEGSTTNATNSSSTTTTASPEEAKMKNCSFNITTNIRNKVQKEYAIFYKSDVIKIDNDTSYYSLISCNTSVLTQACPKVSFEPIPIHYCAPAGFAILKCNDZKFNGSGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVIVRSENFTBNAKTIIVQLNVSVZINCTRPNNNTRKSIHIGPGXAFHTTGAIIGNIRQAYCNISRTEWNNTLXYIAKALGEQFGENKTIAFNKSSGGDPEIVMHSXNCGGEFFYCNTSKLFNSTWFGNGSMKGDDQXNDTIIQLPCKIKQIVNLWQEVGKAMYAPPIQGLIRCSSNITGLLLTRDGGTKNTSEPETFRPGGGNMRDNWRSELYKYKVVKIEPLGIAPXKAKXRVVQREKRAXGLGAMFLGFLGAAGSTMGAASVTLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLALERYLGDQQLLGIWGCSGKLICTTAVPWNNXWSKNKNLSQIWDNLTWMEWEREIDNYTGLIYXLLEESQIQQEKNEKELLELDKWASLWNWFSITNWLWYIKIFIMIVGGLVGLRIVFSVLSIVNRVRQGYSPLSFQTXXPAXRGPDRPEGIEGEGGERDRDRSTPLVNGFLALIWVDLRSLLLF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The presence of Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains is critical for the interaction of the designed protein with its intended targets.2. The designed protein should be expressed specifically in the viral envelope The designed protein sequence is ",
    "response#1": "ITIAADMSGEIKNCSFNTSSITADKKQREFALFYKLDVVPIDNSNNSDNYTSYRLTSCNTSVITQACPKTTFEPIPIHYCTPAGFALLKCNDKTFNGTGLCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSQNLTDNAKNIIVQLNESVPIVCIRPNNNTRRSIRIGPGSAFYATGDIIGDIRQAYCKISKSNWTSALKKIVNKLREQFQNKTIKFNSSSGGDPEITMHTFNCGGEFFYCNSTQLFNSTWDINGNGTGTNETGNDTIILPCRIKQFINMWQGVGKAMYAPPIRGLIKCESNITGLILTRDGGNITGNETFRPGGGDMRDNWRSELYKYKVVRIEPLGLAPTKAKRRVVQREKR",
    "response#2": "MGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLALERYLWDQQLLGIWGCSGKLICTTSVPWNASWSNKSYDTIWQNMTWMQWEREIDNYTGLIYNLLEESQTQQEQNEKELLELDKWANLWNWFSITNWLWYIKIFIMIVGGLVGLRIVFTVLSIVNKVRKGYSPLSFQTR",
    "response#3": "MGAASITLTVQARQSLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQTRVLAIERYLKDQQLLSLWGCSGRLICTTNVPWNSSWSNKSYEDIWDNMTWMQWDKEISNYTDTIYRLLEVSQNQQEQNEKDLLALDKWKDLRNWFNISNWLWYIRIFIMIVGGLIGLRIIFAVLSIVNL"
  },
  {
    "reference": "MGGKWSKRSKGGWSAVREKMEQTEPAAEGVGAASRDLEKHGAITSSNTANTNAACAWLEAQGGEVGFPVRPQVPLRPMTYKAAFDLSHFLKEQGGLEGLIHSQRRQDILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPDQVEKASEGENNCLLHPMNQHGMDDSEREVLMWKFDSRLAFRHMAAELHPHYYKDC",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a specific function in suppression by virus of host autophagy and should be localized to host cell plasma membrane in order to carry out this function efficiently.2. Incorporating a Diacidic; necessary for CD4 internalization motif is critical for the successful design of the protein. The designed protein sequence is ",
    "response#1": "GSSS",
    "response#2": "MSSS",
    "response#3": "MF"
  },
  {
    "reference": "MILREWRRFYTIGSVLLRHGLDELIPRHWQPWPVRLFRRSLFWLRNRYPEQSRGARLRHAFEGLGPVFIKFGQMLSTRRDLLPPDLAEELAMLQDRVPSFDGQLAREQIEQALGQPIEALFADFDQQPLASASVAQVHTARLKENNAEIVIKVIRPDIKPVINDDIRLMRLCAKIVAFLIPNNRLRPVEVIEEYRRTLLDELNLMSEAANAIQLRRNFENSSHLYVPLVYSDYCRESVLVMERIYGIPVSDRAALEANGTDLKLLAERGVEVFFTQVFRDSFFHADMHPGNVFVSYEHPHDPQWIGIDCGIVGTLNRQDKRYLAENFLAFFNRDYRKVAELHVQSGWVPPDTKVEEFESALRTVLEPIFAKPLAEISFGQVLLNLFNTARRFNMHVQPQLVLLQKTLLYIEGLGRHLYPQLDLWQTAKPFLEHWMRQQIGPKAAWRAIKEKAPFWAEKLPDMPDLIYDTLTQVQHQQHMVKGLYQQYHQQHRRHAQARFLLGAGATLLLGSILLLPTHEQLASAGLTISIICWLNGWWKISRR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design must be focused on enhancing the efficiency of phosphorylation, ubiquinone biosynthetic process.2. The protein contains novel Protein kinase domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MLQDRVPPFDSAIARESIEQELNAPIETYFDDFEDTPLASASISQVHTATLKSNGAKVVLKVLRPDVEQKVHADIQLMSQAANFLESLLGANNRLRPAEVVEDYRTTIEGELNLKLEALNAIKLRNNFLDSNALYIPYMYEELCFTRLIVMERIDGVPVSDKAALEAQGTNLKLLAERGVELFFTQVFRDNFFHADMHPGNIFVSREHPNDPFYIGLDCGIMGTLTDEDKRYLAENFLAFFNRDYRRIAQLI",
    "response#2": "LQDRVPPFDPQKAVALIEEQLGAKVGEVFSRFDVEPLASASVAQVHAARLKTGEEVVVKVVRPGLKPVIAQDLAWLFLIAKAAERASADARRLHPVEIVGDYEKTIYDELDLLREAANASQLRRNFEGSELMYVPQVYWDLCRPKVLVMERIYGVPVTDMVTLADQRTDMKLLAERGVEVFFTQVFRDSFFHADMHPGNIFVSTVKPWSPQYIAIDCGIVGSLTAEDQDYLARNLIAFFKRDYRRVAQLR",
    "response#3": "LQDRVAPFDGALARKHIELAMGGPLETWFDDFDQQALASASIAQVHTARLKTTGQEVVLKVIRPDIRPIIKADVRLMYRLAAWVPKLMPDGRRLRPREVVREYEKTLLDELNLLREAANAIQLRRNFDGSPMLYVPEVYSDYCRESVLVMERIYGIPVSDIPTLEKQGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVSYEHPEDPCYIGIDCGIVGSLNKDDKRYLAENFIAFFNRDYRKVAELAV"
  },
  {
    "reference": "MSDTFLEIAYERARQAQQRQMKRRRAAAHRAARGPAPARAPRASPTQRTGTAHEDQALRLLAGAGLVPLARNLHCRAGEIDLVMRDGATLVLVEVRARANPRYGGAAASVGRAKRARLLRCAALLLPDLARRHWGGRIPPVRFDVVAFEAGRADWLPAAFTL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must be optimized for nucleic acid binding. The designed protein sequence is ",
    "response#1": "MTP",
    "response#2": "M",
    "response#3": "MGQ"
  },
  {
    "reference": "MSEVLQKAALAKEAAAEMVMKTTAEKNEALQCIADGLRNERRLILTENQKDIEAGRNRGLTPDIIDRLTLDEKRLLDIADAVELLIGLEDPVGESLETIQKENGLSIEKIRVPLGVVGMIYEARPNVTVDAATLCLKTGNAVVLRGSSSAIHSNIALVSVMKRALGLSKLPIDAVQLIEDTSKETAKQLFTLNDGLDVLIPRGGKNLIDLVVRESTVPVLETGAGNCHVYIDESADPQMASEVVINAKTQRPSVCNAIESLLIHEKWAEEHGRKLLNQLTEKGVELRGDQVICRLEPQAKQAEEADWGAEYLAPILSVKTVQDVQEAVRHIQQYGTNHSEAILTENAEHAAYFQTAVDAAAVYHNASTRFTDGFEFGYGAEIGISTQKLHARGPMGLPALTSTKIIIKGNGQIRV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should interact with enzymes and substrates within the L-glutamate 5-semialdehyde from L-glutamate: step 2/2, Amino-acid biosynthesis, L-proline biosynthesis pathway to enhance or regulate its activity.2. The protein should have a low Km value for L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + L-   glutamyl 5-phosphate + NADPH.3. The protein must exhibit the following characteristics: The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.; Catalyzes the NADPH-dependent reduction of L-glutamate 5- phosphate into L-glutamate 5-semialdehyde and phosphate.4. A protein that contributes to L-proline biosynthetic process by functioning in cytoplasm with NADP binding, glutamate-5-semialdehyde dehydrogenase activity. The designed protein sequence is ",
    "response#1": "MKDMGRKARRAATVLAVMSSEQKNKALEMIALSLEAHSDEILRANHQDLLNATQNNLTVAMVDRLKLNEVRLCTIIDSVRQVACLPDPVGQVMDEWTRPNGLHISRVRTPLGVIGIIYESRPNVTIDASTLCLKAGNAAILRGGSDSFHSAHALHCALVKGLKKAGFPKDAIQMVATKDRGAVGEMLKGLDGAIDVIVPRGGKNLVARIQVDARVPIFAHLEGLCHIYIDQSADLDMARNIVLNAKLRRTGICSAVETVLIDRQALEKFLPILTALQKKGCEIRATEDIVFLMPDAKLAFEEDWSHEYLDAIVSVKTVESVEGAIAHIKRYSSGHTESIIAEDMEIVEKFFNHLDSAILLHNASTQFADGGEFGFGAEIGIATGKMHARGPIGVEQLTSFQYHIKGNGQVRS",
    "response#2": "MKDMGRKARRAATVLAVMSSEQKNKALEMIALSLEAHSDEILRANHQDLLNATQNNLTVAMVDRLKLNEVRLCTIIDSVRQVACLPDPVGQVMDEWTRPNGLHISRVRTPLGVIGIIYESRPNVTIDASTLCLKAGNAAILRGGSDSFHSAHALHCALVKGLKKAGFPKDAIQMVATKDRGAVGEMLKGLDGAIDVIVPRGGKNLVARIQVDARVPIFAHLEGLCHIYIDQSADLDMARNIVLNAKLRRTGICSAVETVLIDRQALEKFLPILTALQKKGCEIRATEDIVFLMPDAKLAFEEDWSHEYLDAIVSVKTVESVEGAIAHIKRYSSGHTESIIAEDMEIVEKFFNHLDSAILLHNASTQFADGGEFGFGAEIGIATGKMHARGPIGVEQLTSFQYHIKGNGQVRS",
    "response#3": "MKDMGRKARRAATVLAVMSSEQKNKALEMIALSLEAHSDEILRANHQDLLNATQNNLTVAMVDRLKLNEVRLCTIIDSVRQVACLPDPVGQVMDEWTRPNGLHISRVRTPLGVIGIIYESRPNVTIDASTLCLKAGNAAILRGGSDSFHSAHALHCALVKGLKKAGFPKDAIQMVATKDRGAVGEMLKGLDGAIDVIVPRGGKNLVARIQVDARVPIFAHLEGLCHIYIDQSADLDMARNIVLNAKLRRTGICSAVETVLIDRQALEKFLPILTALQKKGCEIRATEDIVFLMPDAKLAFEEDWSHEYLDAIVSVKTVESVEGAIAHIKRYSSGHTESIIAEDMEIVEKFFNHLDSAILLHNASTQFADGGEFGFGAEIGIATGKMHARGPIGVEQLTSFQYHIKGNGQVRV"
  },
  {
    "reference": "MPLEIVILAAGQGTRMRSSLPKILHKIGNKSLLEHVYALANRLRPDKITVIYGHGGETVLKSLEHLEVTWVEQREQLGTGHAVMQVADAIGEESTVLILYGDVPLLNDATVKKLLPLAGEDQIGLLTVNLGDPYGYGRIVRDPQGRVLRIVEEKDASAEERETREVNTGILAINGRHLKKWLGRLENNNAQREYYLTDVIAMAVADDVRVETVQPSDPNEVLGVNNRAQLAHLERVFQSGLAEELMRTGVSLRDPSRFDVRGQIDAVGRDVEIDVNVVLEGRIKLGDNVRIGANCVIRDCEIGNNVEILPNCVIEDAVIGEGSRIGPFARIRPETRIDDHVHVGNFVEVKKSAISSGSKVNHLSYIGDTIIGRDVNVGAGTITCNYDGANKHQTVIEDGAFIGSDTQLVAPVRVGKNATIGAGATITKDAPENALTLSRAKQITVEGWQRPVKKRS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that is capable of catalyzing reactions within the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, Nucleotide-sugar biosynthesis pathway with high efficiency and specificity.2. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate are properly positioned for optimal catalysis.3. The designed protein should be involved in cell wall organization, peptidoglycan biosynthetic process, lipid A biosynthetic process, UDP-N-acetylglucosamine biosynthetic process, regulation of cell shape by localizing to cytoplasm and exhibiting UDP-N-acetylglucosamine diphosphorylase activity, glucosamine-1-phosphate N-acetyltransferase activity, magnesium ion binding.4. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.5. The protein should have a high affinity for acetyl-CoA, UDP-N-acetyl-alpha-D-glucosamine, Mg(2+). The designed protein sequence is ",
    "response#1": "MAAGKGTRMRSRWPKVLHRLAGRALVDHVIESAVALQARKVVVITGHGAADVEAAVRRTTAQPSGPADLSFVRQEPQLGTGHAVQQAVPVLPDDGVALILNGDVPLIRAETLRALLQACAGKQGMSLLTVEMPDPTGYGRIVRDAGGAVAAIVEHKDADDAQRAIREVYTGMMAAPTALLKAWLARLDNRNTQNEYYLTDIVAMAVAEGVPVVAHRAADPVEVLGVNDRAQLARLERAHQARQAEALMAAGVTIADPASTWIEDDVTLQPDVTLLPCTQLLGATSVAAGAVIGPDTTLKDVEVGAGARVLRTHGELSVIGPNTDVGPWARLRPGTELGEHGKIGTFVETKNAKIGARSKVPHLSYCGDAILGEAVNIGAGTIFANYDGKHKSTSHLGDDVFIGSNSVLVAPVDIASGAFVAAGSTVTEDIPAGALAVARGGVTLVDPRRIDVRGKLACGRDVLIDINAVFEGEVELGDGVQIGANAVIRDTSIGAGTVIHPHCVLEQSRIGANCQIGPYARTRPGVEMADDAKLGNFVEIKKSRIGKGSKINHLSYIGDAIIGANVNVGAGTITCNYDGANKHRTTIGDGAFIGSGVELVAPVEIGAGATVGAGSTITKAVPADELVLERSQQRTIGNWRRPTKRA",
    "response#2": "MAAGKGTRMRSRWPKVLHRLAGRALVDHVIESAVALQARKVVVITGHGAADVEAAVRRTTAQPSGPADLSFVRQEPQLGTGHAVQQAVPVLPDDGVALILNGDVPLIRAETLRALLQACAGKQGAAGVLTDTVDDPGGYGRIVRDAAGRVVRIVEQKDASSRERSIREVNTGFLAARADLLRPWLGRIGNDNAQGEYYLTDVVAAAVADGVPVVAHRVVDPVQVAGVNDRVALGRLERAHQLRQAEALLDQMAAGKGTRMRSRWPKVLHRLAGRALVDHVIESAVALQARKVVVITGHGAADVEAAVRRTTAQPSGPADLSFVRQEPQLGTGHAVQQAVPVLPDDGVALILNGDVPLIRAETLRALLQACAGKQGRRSWGVTIERPETVTIDVQVCAGMDTIIEPFARLLGSTEIGQDCRIGAGAILESTVLADRVTVAPYTLIADSRIEEGARIGPFARLRMDARVGPDARIGNFVELKKTRLGAGAKSQHLAYLGDAEIGAGSNIGAGTITCNFDGEKKHETKIGAGAFIGSNATLVAPIEIGPESYVGAGSVITDPVPAQALALGRGRQRNIDGWVARKRPGSAAAQAALAAREAAEAAADADADADAHPHPE",
    "response#3": "MAAGKGTRMRSRWPKVLHRLAGRALVDHVIESAVALQARKVVVITGHGAADVEAAVRRTTAQPSGPADLSFVRQEPQLGTGHAVQQAVPVLPDDGVALILNGDVPLIRAETLRALLQACAGKQDIGLLTINLDDPSGYGRILRDREGRVLGIVEEKDASPGQREIREVNTGILAAPTSLLRQWLARLSNNNAQGEYYLTDIIAMAVKDGVEIASAHPADAFEVEGANNRLQLAALERRFQREQAERLMRAGVTVLDPASTWVHAEVDLAVDVTLLPGTTLAGATSVAAGATIGPDTTLIDVEVGENATVMRSHVELAVIGAGATVGPFSYLRPGTELGTGGKVGAFVETKKAVIGAGTKVPHLTYCGDAVLGEGVNIGAGTIFANYDGVTKSTTVVGDNSFVGSDSVLVAPVEIAPGSYVAAGSTITTDVGPGELAVARGQGWKRPVKKPKA"
  },
  {
    "reference": "MARRSRYISLAAVMATLLSALGVTFLLGQGRAEAHGVAMMPGSRTYLCQLDAKTGTGALDPTNPACRSALDTSGATALYNWFAVLDSNAGGRGAGYVPDGTLCSAGNRSPYDFRGYNAARSDWPRTHLTSGSTIQVNYSNWAAHPGDFRVYLTKPGWSPTSELGWDDLELIETVTDPPQRGSAGADGGHYYWDLALPSGRSGDALIFMQWVRSDSQENFFSCSDVVFDGGNGEVTGIRGSGGTPTPTPTPTTPPTTPPPTHSGSCMAVYNVENSWSGGFQGSVEVMNHGTEPLNGWAVQWKPGNGTTLGGVWNGSPTRGTDGTVKVRNVDHNRVVPPDGSVTFGFTATSTGNDFPVGTIGCVAP",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Involved in the degradation of lignocellulosic biomass; Is not active on chitin..2. A protein with cellulose binding functions is required to enable cellulose catabolic process.3. The designed protein have Chitin-binding type-4 domains with specific sequence motifs that are crucial for its activity.4. The Cu cation binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "KWLIGYWHNFDNGSTNIKLRNVSTAYDVINVSFAEPISPGSGTLAFTPYNATVEEFKSDIAYLQSQGKKVLISMGGANGRIELTDATKKRQQFEDSLKSIISTYGFNGLDIDLEGSSLSLNAGDTDFRSPTTPKIVNLINGVKALKSHFGANFVLTAAPETAYVQGGYLNYGGPWGAYLPVIHALRNDLTLLHVQHYNTGSMVGLDGRSYAQGTADFHVAMAQMLLQGFNVGGSSGPFFSPLRPDQIAIGVPASQQAAGGGYTAPAELQKALNYLIKGVSYGGSYTLRQPAGYVGLKGIMTWSINWR",
    "response#2": "AGIAIYWGQNGNEGSLASTCATGNYEFVNIAFLSSFGSGQAPVLNLAGHCNPDNNGCAFLSDEINSCKSQNVKVLLSIGGGAGSYSLSSADDAKQVANFIWNSYLGGQSDSRPLGAAVLDGVDFDIESGSGQFWDVLAQELKNFGQVILSAAPQCPIPDAHLDAAIKTGLFDSVWVQFYNNPPCMFADNADNLLSSWNQWTAFPTSKLYMGLPAAREAAPSGGFIPADVLISQVLPTIKASSNYGGVMLWSKAFDNGYSDSIKCL",
    "response#3": "IGYWHNFDNGSTNIKLRNVSTAYDVINVSFAEPISPGSGTLAFTPYNATVEEFKSDIAYLQSQGKKVLISMGGANGRIELTDATKKRQQFEDSLKSIISTYGFNGLDIDLEGSSLSLNAGDTDFRSPTTPKIVNLINGVKALKSHFGANFVLTAAPETAYVQGGYLNYGGPWGAYLPVIHALRNDLTLLHVQHYNTGSMVGLDGRSYAQGTADFHVAMAQMLLQGFNVGGSSGPFFSPLRPDQIAIGVPAR"
  },
  {
    "reference": "MAPSKKAGKKVTPASKKSAGQGKVAKADWKEGFKKKQVGVSDMTLLTTISNEGVNENLQKRWTNGEIYTYIGAVLISVNPFRGRSVETLQRYRGKNRLEVPPHVFGIAESAYYNMNAYHENQCVIISGESGAGKTEAAKRIMQYIAVVSGGQDSSIQEIKDMVLATNPLLESFGCAKTLRNNNSSRHGKYLEIMFNGVGEPVGAQITNYLLEKGRVVGQIENERNFHIFYQFTKGASDEQRELFGLQGPEAYAYTSLSNCLEVSDIDDVKDYHDTITAMGVIGLTPDEQNEIFKMLAIVLWLGNVQFEEMDDGNSSITDTGVTDFVGYLMEADSALVQKVLTSRVIETSKGGRRGSVYDVPLNPAQATSGRDALAKAIYNNLFEWIVSRINVSMKTRSAHAQIIGILDIFGFEIFEDNSFEQLCINYVNEKLQQIFIELTLKTEQEEYVREQIKWTPIKYFNNKIVCDLIEERRPPGIFAALNDACATAHADPAAADNSFVQRTAMLSSNAHFEARGSQFLVRHYAGDVMYNVAGMTDKNKDSLIKDLLDLVGSSGNTFLQTLFPDRPDPNSKKRPPTAGDRIKARALVDNLMKAQPSYIRTIKPNQNRSSSEYDVKAILHQIKYLGLNENIRVRRAGFAYRNTFEKMVERFYLLSSHTSYAGEYTWTGDSKSGCEQILKDTGIAKDEWQMGVTKAFIKNPETLFALETMRDKYWHNMAARIQRAFRNYMRYKHECARRIQRFWKNNKEGIAYAQTRDYGHQILAGRK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The ATP binding site should be stable and able to withstand changes in environmental conditions.2. For general function, the protein need meet that Required for proper actin cytoskeleton polarization; Functions as actin nucleation-promoting factor (NPF) for the Arp2/3 complex .3. The protein should be optimized for myosin complex-specific folding and stability, while maintaining actin binding, ATP binding, cytoskeletal motor activity.4. The designed protein should contain one or more Myosin motor, TH1, SH3 domains that are essential for its function.5. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "PSLLYNLRVRYSQDLIYSKAGPVLIAVNPFKNVQIYGEEFLSAYQKNALDAPHVYAVADAAYDDMMREEKNQSIIISGESGAGKTETAKYAMQYLEALGGGSFGVENEILKTNCILEAFGNAKTSRNDNSSRFGKLMEIHFSAKGKICGAKLETFSLDQSRVAQLCNGERCYHIFYQLCAGASPILKERLKIKAASEYNYLNQSNCLTIDRTDDAQKFHKLMEAFNIVQIPQEYQERTFALLAAVLWLGNVSFEVIDNENHVEVVADEAVTNVAMLMGCNSKKLMVVLSTCKLQAGRDCIAKRLTLRQATDMRDSLAKIIYASLFNWLVEQINISLEVGNSRTGRSISILDIYGFESFKDNSFEQFCINYANERLQQHFNRHLFKLEQEEYEGDGIDWTKVEFIDNQECLNLIEKKPIGLVSLLNEESNFPKATDTTFANKLKQHLNANSCFKGERGRGFRIKHYAGEVLYNTNGFLEKNRDPLHVDLIQLLSLCKCQLLNLFSTKMHHDFLKPATFSDSMNQSVIAKFKGQLFKLMNKLEDTTPHFIRCIKPNSNQLPGLYEENHVLQQLRCCGVLEIVRISRSGYPTRRLTARDRVGVQDFVLLENFTSEAAFIENLRKRFKENLIYTYIGSVLVSVNPYKELEIYSKQNMERYRGVSFYEVSPHLYAIADNSYRSLRTERKDQCILISGESGAGKTEATKKILQYYAVTCPASQQVETVKDRLLQSNPVLEAFGNTKTLRNDNSSRFGKYMDVQFDYRGAPVGGHILNYLLEKSRVVHQNHGERNFHIFYQLLEGGEEDLLRRLGLEKNPQQYHYLVKGHCARVSSINDKNDWKVVRRALSIISFNDNEVEDLLSIVASVLHLGNVQFAADEQGNAQVTTENQIKYLARLLAVEGSVLRDALIHKKIIAKGEELISPLNLEQAAYARDALAKAIYGRTFSWLVNKVNKSLAYKEGEFPGWRSTTVLGLLDIYGFEVFQHNSFEQFCINYCNEKLQQLFIELTLKSEQEEYESEGIAWEPVQYFNNKIICDLVEEKFKGIISILDEECLRPGDATDTTFLEKLEETVKNHPHFLTHKLADQKTRKSLGREEFRLLHYAGEVTYSVAGFLDKNNDLLFRNLKETMCNSENPIINQCFDRTELTDKKRPETAATQFKNSLSKLMEILMSKEPSYIRCIKPNDAKQADRFDEVLIRHQVKYLGLMENLRVRRAGFAYRRKYEVFLQRYKSLCPETWPTWDGRPHDGVAVLVKHLGYKQEEYKMGRTKIFIRFPKTLFATEDALE",
    "response#2": "DMQDLIHLSGPLTEHAVMRTLQARFNERRYFTNVGPILLSINPYLDVGNPLTLTSTRAMPLAPQLQKIVQEAVRQQSETGYPQAIILSGTSGAGKTANAMLMLRQLFAIAGGGPETDAFKHLAAAFTVLRSLGSAKTTTNSESSRIGQFIEVQVTDGALYRTKIHCYFLDQTRVIRPLPKEKNYHIFYQLLAGLSREERQKLHLDGYSPANLRYLRGDIGQNEQEDAARFQAWKTCLGILGIPFLDVVRVLAAVLLLGNVQFIDGGGLEVDVKGETELNSVASLLGVPPAALFRGLTTRTHNVRGQLVKSVCGDGDANMTRDCLAKALYCRTVATIVRRANSLKRLGSTLGTLSSDSNESVHNQADVASQHASTIGGGNAGSKSMAALNNAVRHATSDGFIGILDMFGFEEPSPHAHLEHLCINH",
    "response#3": "GNSSPKDMQDLIHLSGPLTEHAVMRTLQARFNERRYFTNVGPILLSINPYLDVGNPLTLTSTRAMPLAPQLQKIVQEAVRQQSETGYPQAIILSGTSGAGKTANAMLMLRQLFAIAGGGPETDAFKHLAAAFTVLRSLGSAKTTTNSESSRIGQFIEVQVTDGALYRTKIHCYFLDQTRVIRPLPKEKNYHIFYQLLAGLSREERQKLHLDGYSPANLRYLRGDIGQNEQEDAARFQAWKTCLGILGIPFLDVVRVLAAVLLLGNVQFIDGGGLEVDVKGETELNSVASLLGVPPAALFRGLTTRTHNVRGQLVKSVCGDGDANMTRDCLAKALYCRTVATIVRRANSLKRLGSTLGTLSSDSNESVHNQADVASQHASTIGGGNAGSKSMAALNNAVRHATSDGFIGILDMFGFEEPSPHAHLEHLCIHSAVEIQSWWRGTIGRRH"
  },
  {
    "reference": "MVIIPAIDLKGGKCVRLLQGDFERVTVYSDHPVEMAKAWREKGAERLHLVDLDGSIAGNPRNAAIISQIVKSVGVPVEIGGGIRDISTIQRYLDMGVQWVILGTAALKDRSFVYNACDLFPGHVILGIDANNGKVAVEGWTEQSAITALELAISYENRGIAAVIYTDISRDGMQTGVNVEGTRVLAEAVDIPVIASGGVATLDDIKRLLPLEESGIAGVIIGKALYSGAIALEEAISLAKSS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must have a Proton donor active site that is highly conserved among related enzymes.2. The protein must be able to 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity.3. Ideal protein should have a binding affinity for key enzymes in the L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9, Amino-acid biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "KXNFVRL",
    "response#2": "MXTGSLRLXG",
    "response#3": "MXIXGXKXGF"
  },
  {
    "reference": "MNISVINLFFNGTKIMGFRQFNSARFLHKSSTHFATQVPISRFELDKFLPYDDLIQRLDIVRKRLNRPLTLAEKILYSHLDDPENAEIVRGSSYLKLRPDRVAMQDATAQMAVLQFISSGLNSVSVPTTIHCDHLIEANEEANSDLKRAKDVNAEVYEFLSSVAAKYGIGFWHPGSGIIHQIILENYAFPGLLLIGTDSHTPNGGGLCGLCIGVGGADAVDVMADIPWELKCPKVMGVKLQGQLSGWTSPKDVILKLAEILTVKGGTGYIIEYFGPGVDSISCTGMGTICNMGAEVGATTSVFPFTNRMKRYLEATGREGIATAAEKCKHLFTSDAGAGYDQLVEINLSELEPRINGPFTPDLGHTIHNLGQHARENGYPLEVKAGLIGSCTNSSYEDMTRAANVAQQAVEHNYKAKSIFDVTPGSEQIRATMERDGLTETFRKIGATVLANACGPCIGQWNRKDVRKGEKNTIVTSYNRNFTGRNDANPATHAFVASPEIVTAIALAGRLDFDPTRDYLTANDGRKFKLKVPVGEELPSKGFDRGQETYQAPPADGSAVKVVVQPNSKRLQLLKAFDKWDGKDFEDMFVLIKIKGKCTTDHISAAGPWLKFRGHLDNISNNMFIGAINEENGEMNKVKNQLSGEWSSVPEAARFYKAKGKKWIVFGEENYGEGSSREHAALEPRHLGGRAIVVKSFARIHETNLKKQGMLALTFVNPADYHKVLPTDSVSLVGLKDFKPGKPLKCILKHHDGSTDEFLLDHTYNDLQ",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Ensure the presence of a signal peptide within the protein.2. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding.3. The desired protein should be able to perform citrate = D-threo-isocitrate4. The protein should be optimized for activity in tricarboxylic acid cycle pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. For general function, the protein need meet that Catalyzes the isomerization of citrate to isocitrate via cis- aconitate.6. The protein contains novel Zinc-hook domains that confer a unique function or activity.7. A protein that contributes to tricarboxylic acid cycle, DNA repair, telomere maintenance, DNA recombination by functioning in Mre11 complex with ATP-dependent DNA damage sensor activity, citrate dehydratase activity, aconitate hydratase activity, ATP hydrolysis activity, metal ion binding. The designed protein sequence is ",
    "response#1": "EKVLYSHLDDPANQDIVRGTSYLRLRPDRVAMQDATAQMALLQFISSGLKKVAVPSTVHCDHLIEAQIGGPKDLARAKDLNKEVYDFLASTCAKYGLGFWKPGSGIIHQIILENYAFPGLLMIGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVNLTGKISGWTSPKDVILKVADILTVKGGTGAIIEYHGKGVDSISCTGMATICNMGAEIGATTSLFPFNQRMADYLKSTGRAGIASEAQKYQAKILSADKNCEYDELIEINLDTLEPHVNGPFTPDLGHPISKLGENSKKNGYPMDIRVGLIGSCTNSSYEDMGRCASIAKDAMSHGLKSKIPFNVTPGSEQIRATIERDGISEVFDKFGGTVLANACGPCIGQWDRKDVKKGDKNTIVTSYNRNFTGRNDANPATHCFVTSPELVTALSIAGRIACPELSQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQAYRQHVAERAALGIPPLPLSAAQTAELIELLKVPPAGEGANLVYLLTHRVPAGVDDAAKVKASYLAAVAHGTEPNSLISRELATELLGTMLGGYNISPLIDLLDDAAVGAVAAKGLKGTLLMFDQFHDVKAKADKGNDHARAVLQSWADAEW",
    "response#2": "GISLRPGDGVIHSWLNRFLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGTMQPGVTLRDLVNAIPLYAIKQGLLTVDKKGKKNIFSGRILEIEGLPELKVEQAFELSDASAERSAAGCTVHLNKEPIIEYINSNITLMKWMIAQGYADARTLERRIAAQEAWLANPQLLKGDADADYAAVIEIDLADVHEPIVACPNDPDDVKTLAEVSGAKIDEVFIGSCMTNIGHFRAASKLLEGKRDIPVKLWVAPPTKMDAHQLTEEGHYGVLGTAGAGVMCIKVLGGSHRRYAAVGDLVVAVIKEATPNMPLERSEVIRVVIAAITSCTNTSNPSLRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIPGAGTLEIDNNCQGILGYVVRWIDQGVGCSKVPDIHDVGLMEDRATLRISSQHLANWLHQNVVTSDQVMDSLKRMAVVVDKQNAGDPLYTPMAPTFDGVAFKAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQ",
    "response#3": "GISLRPGDGVIHSWLNRFLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGTMQPGVTLRDLVNAIPLYAIKQGLLTVDKKGKKNIFSGRILEIEGLPELKVEQAFELSDASAERSAAGCTVHLNKEPIIEYINSNITLMKWMIAQGYADARTLERRIAAQEAWLANPQLLKGDADADYAAVIEIDLADVHEPIVACPNDPDDVKTLAEVSGAKIDEVFIGSCMTNIGHFRAASKLLEGKRDIPVKLWVAPPTKMDAHQLTEEGHYGVLGTAGAGVMVGMGQKDSPHINGPFTPDLATPGQRCPHVNGPFTPDLGHPQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQETKRDLISEACKSLCYVALDFESEMNAASSSSSVEKSYELPDGQVITIGNERFRCPEALFHPSFLGMETAGIHEATYNSIMKCDVDIRKDLYGNIVLSGGTTMFPGMGDRLSKEITALAPSSMKVKIIAPPERKYSAWIGGSILASLSTFQQMIGFWKPGSEIDEEAINGLFQKYDKNGDGFIDFEEFTRMLVKMNLE"
  },
  {
    "reference": "MAGVCASSLKLSLPNVLRNALRSEFASDVHQGPASRRILSITPFSQSRYKTQRRNFSASIKPQLCQSAIYLSAQDPSSSASFSSNTAPLGGSENEIRRASTEANASPERNDTKTSHADYLAEAPSSDTPTDKKRTKSSRSKKRSRHEEIPSNPMPQKKPEKWQLHKQALKEKFKDGWNPSKKLSPDALEGIRHLHAVAPEKFTTPVLAEEFKVSPEAIRRILKSKWRPSETEIEDRRKRWERRHDRIWGHLSELGLRPSTKRTRDLTDANQLLYGNNQKKGGKA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should exhibit Polar residues, Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "VLASGAAILPHPSKAATGGEDAYFIACDGWFGVADGVGQWSFEGINAGLYARELMDGCKKFIMENQGAADIKPEQVLSKAADEAHSPGSSTVLVAHFDGQFLNASNIGDSGFLVIRNGEVYQKSKPMVYGFNFPLQIEKGDNPLKLVQNYTIELEDGDVIVTASDGLFDNVYEQEVATMVSKSLQADLKPTEIAEHLAAKAQEVGRSAAGSTPFSDAALAVGYLGFSGGKLDDIAVVVW",
    "response#2": "VLASGAAILPHPSKAATGGEDAYFIACDGWFGVADGVGQWSFEGINAGLYARELMDGCKKFIMENQGAADIKPEQVLSKAADEAHSPGSSTVLVAHFDGQFLNASNIGDSGFLVIRNGEVYQKSKPMVYGFNFPLQIEKGDNPLKLVQNYTIELEDGDVIVTASDGLFDNVYEQEVATMVSKSLQADLKPTEIAEHLAAKAQEVGRSAAGSTPFSDAALAVGYLGFSGGKLDDIAVVVL",
    "response#3": "ASGAAILPHPSKAATGGEDAYFIACDGWFGVADGVGQWSFEGINAGLYARELMDGCKKFIMENQGAADIKPEQVLSKAADEAHSPGSSTVLVAHFDGQFLNASNIGDSGFLVIRNGEVYQKSKPMVYGFNFPLQIEKGDNPLKLVQNYTIELEDGDVIVTASDGLFDNVYEQEVATMVSKSLQADLKPTEIAEHLAAKAQEVGRSAAGSTPFSDAALAVGYLGFSGGKLDDIAVVVSIL"
  },
  {
    "reference": "MSDYLELLPAVDITEGRAVQLAQGVAGSERTYGDPVAAAMRWQEAGAEWIHLVDLDAAFGRGSNRELQAEIVGALDVQVEMSGGIRDDESLAAALATGCRRVNIGTAALEQPEWCRRVIAEHGDRVAIGLDVRGRTLAARGWTKDGGDLYETLARLDAEGCARYVVTDVNKDGMLQGPNLQLLRDVCAATSAPVVASGGVTTLADIEALMELVPVGVEGAIAGTALYEGRFTLEDALALTRGGR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must be optimized for 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity.2. The protein should have a low Km value for 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-   ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-   phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-   ribosyl)imidazole-4-carboxamide. The designed protein sequence is ",
    "response#1": "RVGVIMGSDSDWSVMQDAAHALAEFDIPIEVRVVSAHRTPAEMFDYARNAVDRSIAVIIAGAGGAAHLPGMVASATPLPVIGVPVPLARLDGLDSLLSIVQMPAGVPVATVSIGGARNAGLLAVRILGSSDLQLRAQLVAFQDRLAAWFAAQPGLHSGKVRDVYADGDDLILVASDRVSVYDVVLPTPIPEKGKLLTALSLWWFDQLAELVPNHVLSATDVPVELAGRAIRCRRLEMVPVECVARGYLVGGGFAEYQRTGVVSGIELPRGMVEAAALPEPIFTPSTKAPVGEHDQPMTFGEVVDKVGAETAERLRQITLDVYRRGAELAADRGILIADTKIELGWAADGTLTVGDELLTSDSSRFWPAESYQPGRAQFSYDKQYVRDWATRSGWDRAAS",
    "response#2": "MELLHSGKVRDVYADGDDLILVASDRVSVYDVVLPTPIPEKGKLLTALSLWWFDQLAELVPNHVLSATDVPVELAGRAIRCRRLEMVPVECVARGYLVGGGFAEYQRTGVVSGIELPRGMVEAAALPEPIFTPSTKAPVGEHDQPMTFGEVVDKVGAETAERLRQITLDVYRRGAELAADRGILIADTKIELGWAADGTLTVGDELLTSDSSRFWPAESYQPGRAQFSYDKQYVRDWATRSGWDRRSPAPEVPDEVVDATRARYVDVYERLTA",
    "response#3": "MELLHSGKVRDVYADGDDLILVASDRVSVYDVVLPTPIPEKGKLLTALSLWWFDQLAELVPNHVLSATDVPVELAGRAIRCRRLEMVPVECVARGYLVGGGFAEYQRTGVVSGIELPRGMVEAAALPEPIFTPSTKAPVGEHDQPMTFGEVVDKVGAETAERLRQITLDVYRRGAELAADRGILIADTKIELGWAADGTLTVGDELLTSDSSRFWPAESYQPGRAQFSYDKQYVRDWATRSGWDRRSPAPEVPDEVVDATRARYVDVYERLTS"
  },
  {
    "reference": "MRGARAVIDLRAIGYNYNLIRELLDEKNPDAHILCILKANAYGHGAVEVAQFLAKYCSAEGFGVASIEEALELRLSGIQNRILLLEGFFTDEDELSLIDKYNFSITIHCEEQLKSFMNYPFKKPVEVHLKLDSGMNRLGFTPAEYADKYRLLKNHKNVSGIVKATHFAFADIPEKSEYTLKQWRIFEKAAGCLPDPLSAGGGVIVVGWLNTIHMDWLRTGSMLYGLDPYDLDAKAPELPKPLIPAMKLMSTIVCVKHVEKDQPIGYGGAYVTTRDSLIGVVAIGYGDGFPQVRNGCPVIINGKRVPTVGKVCMDMLAIDVTDVPDVKRGDDVVLWGNPELTIEEVSTFSNENPFEIITGLTRRVPLQYTF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein requires pyridoxal 5'-phosphate to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "SGYLPNKNSVRCMGTYYAKFKEANPEMIWLMDPVMGDEGQLYVSEDVIPEYRKLALSPKQLVDIITPNQFELEILYGGEIKTKEHLKKALKKLHQTIPVIIVTSCDCKMFDDKDFIYCVASMEGKTPIVYRVPFIDSYFTGVGDLFSALLY",
    "response#2": "SGYLPNKNSVRCMGTYYAKFKEANPEMIWLMDPVMGDEGQLYVSEDVIPEYRKLALSPKQLVDIITPNQFELEILYGGEIKTKEHLKKALKKLHQTIPVIIVTSCDCKMFDDKDFIYCVASMEGKTPIVYRVPFIDSYFTGVGDLFSALLF",
    "response#3": "SGYLPNKNSVRCMGTYYAKFKEANPEMIWLMDPVMGDEGQLYVSEDVIPEYRKLALSPKQLVDIITPNQFELEILYGGEIKTKEHLKKALKKLHQTIPVIIVTSCDCKMFDDKDFIYCVASMEGKTPIVYRVPFIDSYFTGVGDLFSALLS"
  },
  {
    "reference": "MKQLSSAEVRQLFLDFFKEKGHTIEPSAPLVPNNDPTILWINSGVATMKKYFDGSVIPDNPRMANAQKSIRTNDIENVGKTARHHTFFEMLGNFSIGDYFKEGAIVFAWEFLTSPKWIGFDPDKLYVTVYPEDEEAKTLWREKIGLSDDHIVEIEDNFWDIGIGPSGPDSEIFYDRGPAFGDDASDPELYPGGENERYLEIWNLVFSQFNHNPDGTYTPLPKQNIDTGMGLERMVSIIQDAPTNFETDLFMPIIREVEQIAGVKYGHSQENDVAFKVIADHIRTVAFAIGDGALPSNEGRGYILRRLLRRAVRYAKVLTINEPFMYKLVPVVGKIMNSFYPEVENQTDFIQKVIRTEEERFHETLNEGLAILETILKTAKETNEQVIKGADIFKLYDTFGFPVELTEEYAEDHGLKVDHAGFEAEMKEQRARARAARADVKSMQVQGELLANLTEKSAFVGYNSTEHVSEILYLIQNDTLVDEVAAGNEAQVIFKETPFYAESGGQVADKGTIESETGLAYVEDVQKAPNKQNLHRISVKEGVLKTGDTVKLAVDKVKRRETIKNHTATHLLHRALKDTLGEHVNQAGSLVSPDRLRFDFSHFGQITEEELTKMEEIVNEKIWEQINVVIEEMPIAEAKELGAMALFGEKYGDIVRVVQVGKYSIELCGGVHVRNTADIGLFKIVSETGIGAGTRRIEAVTGKEAYRFVTEQENTLKQAASLLKTTTKETPQKVELLQADLREVKRENESLLSKLASAASADIFESPE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must have high specificity towards zinc ion binding. The designed protein sequence is ",
    "response#1": "GVGHMGVKIAKAMGHHVTVISSSNKKREEALQDLGADDYVIGSDQAKMSELADSLDYVIDTVPVHHALEPYLSLLKLDGKLILMGVINNPLQFLTPLLMLGRKVITGSFIGSMKETEEMLEFCG",
    "response#2": "GILSPYTYTLRETGPEDVNIRIICCGICHTDLHQTKNDLGMSNYPMVPGHEVVGEVVEVGSDVSKFTVGDIVGVGCLVGCCGGCSPCERDLEQYCPKKIWSYNDVYINGQPTQGGFAKATVVHQKFVVKIPEGMAVEQAAPLLCAGVTVYSPLSHFGLKQPGLRGGILGLGGVGHMGVKIAKAMGHHVTVISSSNKKREEALQDLGADDYVIGSDQAKMSELADSLDYVIDTVPVHHALEPYLSLLKLDGKLILMGVINNPLQFLTPLLMLGRKVITGSFIGSMKETEEMLEFCKEKGLSSIIEVVKMDYVNTAFERLEKNDVRYRFVY",
    "response#3": "WKSVIPFRFKGGDAKWKKLIREGLGLWEKETCVRWSENGPGKDYVIFFRGSGCYSSVGRTGGSQLISIGYGCEDKGIVAHEVGHSLGFWHEQSRPDRDDYIHLRKDWIIKGTDGNFEKRSWEEIEDMGVPYDVGSVMHYGSNAFTKDWDQITIETKDSRYQGTIGQRQKLSFIDVKQVNRLYCS"
  },
  {
    "reference": "MTINTKNIVVVGAGVFGVSVANHLYRELGGTYAIKLVTASNYVYFLPSAVRLTVSKDYTKSILPLKNVLDSGIEVIKDTAASFDDKEVVLGSDRAIKFDILVLATGSKWADPIGSTYTFGDNYKEYFEREASRISDADHILFLGGGFVNCELAGELLFKYLEEIRSGKKRISIIHNSDKLLPDSGLYNDTLRKNVTDYLSKNGITLYLNTVGASLDTSPKRIFLGEGSSKYIDADLIYRGVGISPNVPVNSISDLCDKKGFIQVEKNFRVKAVEAGNVFAIGDVTNFRYHGLVKRDNWVDVLTRNVISSLQEGTEASLVDADCLETGHAPSGVSLGPNAGFGQFPLPLLGTINIPSFLISRAKSKNLFSDKMEPLFKK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be optimized for stability and expression in mitochondrion, nucleus, mitochondrial outer membrane where it will participate in apoptotic process, positive regulation of apoptotic process, aerobic electron transport chain.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. The protein requires FAD to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "NDLPAPTNISMWWNLGSLLGMCLMIQIVTGLFLTMHYTPNIEMAFSSVVHICRDVNNGWLIRTLHANGASMFFICMYLHVGRGIYYGSYMYMNTWMTGTIILFLVMATAFMGYVLPWGQMSFWGATVITNLLSAIPYIGTDIVQWVWGGFAVDNATLNRFYTFHFVLPFIVMAMVMIHLFFLHQTD",
    "response#2": "PANPLNTPPHIKPEWYFLFAYAILRSIPNKLGGVIALALSILILAFLPYLHTSNQRSMMFRPLSQVLYWTLVTDLLLLTWIGGQPVEPPFIIIGQLASILYS",
    "response#3": "NPIGLNSNIDKIPFHPYFTYKDMITFIILMMILIMLCLIDPYMLGDPDNFVPANPLVTPIHIQPEWYFLFAYAILRSIPNKLGGVIALVMSISILMIMPFYNKTKFRGNQFYPMNQIMFWIMVIVICLLTWIGKRPVEEPYIMTGQR"
  },
  {
    "reference": "MRRTITTQLAATALGLILAGAAWADPVEDTLVIDTDDGELEIVTTAPAPDHLKDVMDTIWSGWHYREDETRDLQRDDFDNPAMVFADRGLDRWNAEIGANGESCASCHQGPETFKGLRAVMPRVDEKTGKLMIMEDYIQSCVTERMGLEPWKITGDQMKDMLALISMQSRGEIVNVAIDGPAQPFWEKGKELYYTRFGQLEMACANCHEDNQGMMIRADHLSQGQINGFPTYRLKDAGMVSAQQRFVGCVRDTRAETFAAGSDEFKAIELYVASRGNGLAVEGVSVRH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a binding site that is specific to the target heme c ligand.2. The designed protein should contain one or more Cytochrome c domains that are essential for its function.3. The Cysteine persulfide intermediate of the protein must be engineered to enable effective catalysis of 2 Fe(III)-[cytochrome c] + S-sulfanyl-L-cysteinyl-[SoxY   protein] + thiosulfate = 2 Fe(II)-[cytochrome c] + 2 H(+) + S-(2-   sulfodisulfanyl)-L-cysteinyl-[SoxY protein].4. The protein can be targeted to periplasmic space, cytochrome complex and perform metal ion binding, heme binding, oxidoreductase activity, acting on a sulfur group of donors, cytochrome as acceptor.5. The protein must exhibit the following characteristics: C-type diheme cytochrome, which is part of the SoxAX cytochrome complex involved in sulfur oxidation; Some electrons may be used for reductive CO(2) fixation.; This leads to the liberation of two electrons, which may be transferred from the SoxAX complex to another cytochrome c that then channels them into the respiratory electron transport chain.6. The presence of heme is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MNTATSTAYSYKVVRQFAIMTVVWGIVGMGLGVFIAAQLAWPFLNFDLPWTSFGRLRPLHTNAVIFAFGGCALFATSYYSVQRTCQTTLFAPKLAAFTFWGWQLVILLAAISLPLGFTSSKEYAELEWPIDILITIVWVAYAVVFFGTLAKRKVKHIYVGNWFFGAFILTVAILHVVNNLEIPVTAMKSYSLYAGATDAMVQWWYGHNAVGFFLTAGFLGIMYYFVPKQAERPVYSYRLSIVHFWALITVYIWAGPHHLHYTALPDWAQSLGMVMSLILLAPSWGGMINGMMTLSGAWHKLRSDPILRFLVVSLAFYGMSTFEGPMMAIKTVNALSHYTDWTIGHVHAGALGWVAMVSIGALYHLVPKVFGREQMHSIGLINTHFWLATIGTVLYIASMWVNGIAQGLMWRAINDDGTLTYSFVESLEASHPGFVVRMIGGAIFFAGMLVMAYNTWRTVQAAKPAEYDAAAQIA",
    "response#2": "MNTATSTAYSYKVVRQFAIMTVVWGIVGMGLGVFIAAQLAWPFLNFDLPWTSFGRLRPLHTNAVIFAFGGCALFATSYYSVQRTCQTTLFAPKLAAFTFWGWQLVILLAAISLPLGFTSSKEYAELEWPIDILITIVWVAYAVVFFGTLAKRKVKHIYVGNWFFGAFILTVAILHVVNNLEIPVTAMKSYSLYAGATDAMVQWWYGHNAVGFFLTAGFLGIMYYFVPKQAERPVYSYRLSIVHFWALITVYIWAGPHHLHYTALPDWAQSLGMVMSLILLAPSWGGMINGMMTLSGAWHKLRSDPILRFLVVSLAFYGMSTFEGPMMAIKTVNALSHYTDWTIGHVHAGALGWVAMVSIGALYHLVPKVFGREQMHSIGLINTHFWLATIGTVLYIASMWVNGIAQGLMWRAINDDGTLTYSFVESLEASHPGFVVRMIGGAIFFAGMLVMAYNTWRTVQAAKPAEYDAAAQIAS",
    "response#3": "MNTATSTAYSYKVVRQFAIMTVVWGIVGMGLGVFIAAQLAWPFLNFDLPWTSFGRLRPLHTNAVIFAFGGCALFATSYYSVQRTCQTTLFAPKLAAFTFWGWQLVILLAAISLPLGFTSSKEYAELEWPIDILITIVWVAYAVVFFGTLAKRKVKHIYVGNWFFGAFILTVAILHVVNNLEIPVTAMKSYSLYAGATDAMVQWWYGHNAVGFFLTAGFLGIMYYFVPKQAERPVYSYRLSIVHFWALITVYIWAGPHHLHYTALPDWAQSLGMVMSLILLAPSWGGMINGMMTLSGAWHKLRSDPILRFLVVSLAFYGMSTFEGPMMAIKTVNALSHYTDWTIGHVHAGALGWVAMVSIGALYHLVPKVFGREQMHSIGLINTHFWLATIGTVLYIASMWVNGIAQGLMWRAINDDGTLTYSFVESLEASHPGFVVRMIGGAIFFAGMLVMAYNTWRTVQAAKPAEYDAAAQIN"
  },
  {
    "reference": "MESPAFFFTLFLWFFLLSITIYSIYIGFGPPSKRLRDPFEEHED",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: May play a role in photosystem I and II biogenesis..2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.3. The protein should be optimized for stability and solubility when expressed in chloroplast thylakoid membrane where it will be involved in photosynthesis. The designed protein sequence is ",
    "response#1": "IIQHGGDPQVGNLATPINASAFSKAFINNLPGYRQGLSAQRRGLEVGMAHGYFLYGPFALLGPLRNADFAGVAGLLGTVGLISLLTLALSLYGSVGVSTPTATLTTPNPPENLGTKEGWSEFAAGFLIGGCGGALVAYGLCR",
    "response#2": "IIQHGGDPQVGNLATPINASAFSKAFINNLPGYRQGLSAQRRGLEVGMAHGYFLYGPFALLGPLRNADFAGVAGLLGTVGLISLLTLALSLYGSVGVSTPTATLTTPNPPENLGTKEGWSEFAAGFLIGGCGGALVAYGLCG",
    "response#3": "IIQHGGDPQVGNLATPINASAFSKAFINNLPGYRQGLSAQRRGLEVGMAHGYFLYGPFALLGPLRNADFAGVAGLLGTVGLISLLTLALSLYGSVGVSTPTATLTTPNPPENLGTKEGWSEFAAGFLIGGCGGALVAYGLCI"
  },
  {
    "reference": "NYHVLLGQNNLSEDVQHRLVSQSFRHPDYKPFLMRNHTRKPKDYSNDLMLLHLSEPADITDGVKVIDLPTKEPKVGSTCLVSGWGSTNPSEWEFPDDLQCVNIHLLSNEKCIKAYKEKVTDLMLCAGELEGGKDTCRGDSGGPLICDGVLQGITSWGSVPCGEPNKPGIYTKLIKFTSWIKEVMKENP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be able to regulate regulation of systemic arterial blood pressure.2. The protein have enhanced Preferential cleavage of Arg-|-Xaa bonds in small molecule   substrates. Highly selective action to release kallidin (lysyl-   bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-   Xaa.. The designed protein sequence is ",
    "response#1": "IVGGWECSQPWQAALYHFSTFQCGGILVHPQWVLTAAHCIGDNYQLWLGRHNLFDDEDTAQFVHVSESFPHPCFNMSLLKNHTRQADEDYSHDLMLLRLTQPAEITDAVQVVELPTQEPEVGSTCLASGWGSIEPENFSYPDDLQCVDLKILPNDKCAKAHTQKVTEFMLCAGHLEGGKDTCVGDSGGPLTCDGVLQGVTSWGYIPCGSPNKPAVFVRVLSYVKWIEDIVGGWECSQPWQAALYHFSTFQCGGILVHPQWVLTAAHCIGDNYQLWLGRHNLFDDEDTAQFVHVSESFPHPCFNMSLLKNHTRQADEDYSHDLMLLRLTQPAEITDAVQVVELPTQEPEVGSTCLASGWGSIEPENFSYPDDLQCVDLKILPNDKCAKAHTQKVTEFMLCAGHLEGGKDTCVGDSGGPLTCDGVLQGVTSWGYIPCGSPNKPAVFVRVLSYVKWIEDTIK",
    "response#2": "IVGGWECSQPWQAALYHFSTFQCGGILVHPQWVLTAAHCIGDNYQLWLGRHNLFDDEDTAQFVHVSESFPHPCFNMSLLKNHTRQADEDYSHDLMLLRLTQPAEITDAVQVVELPTQEPEVGSTCLASGWGSIEPENFSYPDDLQCVDLKILPNDKCAKAHTQKVTEFMLCAGHLEGGKDTCVGDSGGPLTCDGVLQGVTSWGYIPCGSPNKPAVFVRVLSYVKWIEDTIR",
    "response#3": "IVGGWECSQPWQAALYHFSTFQCGGILVHPQWVLTAAHCIGDNYQLWLGRHNLFDDEDTAQFVHVSESFPHPCFNMSLLKNHTRQADEDYSHDLMLLRLTQPAEITDAVQVVELPTQEPEVGSTCLASGWGSIEPENFSYPDDLQCVDLKILPNDKCAKAHTQKVTEFMLCAGHLEGGKDTCVGDSGGPLTCDGVLQGVTSWGYIPCGSPNKPAVFVRVLSYVKWIEDTIR"
  },
  {
    "reference": "MSSHSKQALPAITVAAIGVVFGDIGTSPLYALKEIFNGHHPIPVTPENILGVLSLVFWAIIVLVTIKYVAIIMRADNRGEGGSLALLALVTERAKNPRLSWIITLLGIFAAALFYGDSMITPAISVLSAVEGLEIITPDLKSYVIPITLGILTGLFFIQKHGTGAVGKLFGPVMVAWFGILAILGLKEIAYNPAVLLALNPLFAIVFVAEHTGLAFLALGSVVLAVTGGEALYTDMGHFGRFPIRLAWFGFVMPALVLNYFGQGALLLIEPEAIASPFFHLAPDWALIPMVGLATAATVIASQAVISGAFSVARQSIQMGLLPRMQIIHTSGMEEGQIYVPFTNWSLYLAVIALVIGFKNSSNLAAAYGIAVTGTMLIDTILVAFVMVLMWKWNKLLVALVAGTLLLVDIAFFAANAIKIPEGGWFPLAMGLVSFTVLTTWRRGRRMVSEEMAKQSIPMSDFLQSIDDVHRIYGTAIFMTSAKDGVPPALLHNLKHNQVLHERVVLVTVQTTDTPTVNDMERIYLHRMQKGFMRLIVRYGFMESPDIPGALELCKGHGERFDMMETTFYLSRETIVPSMARGMLPWRARLFAVMSKNATSASDFFHIPTNRVVELGTQLVI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Likely operates as a K(+):H(+) symporter..2. The designed protein must be able to perform potassium ion transmembrane transporter activity, symporter activity under specific conditions. The designed protein sequence is ",
    "response#1": "IVYLTSDPEMKRVDTDIFFSIFADHPKRARAWWAVSVETSDNPFTREYSVENFGTDFLFRVRIRLGFKVSQSIPAYIHQIMNDLSKSGDLPKQTTRYPKLDADPNIGPIRYVLIHKALMPESKVSQKGAISLQIKYAIRHLAGSPVKWFGLAPYNPLIF",
    "response#2": "IVYLTSDPEMKRVDTDIFFSIFADHPKRARAWWAVSVETSDNPFTREYSVENFGTDFLFRVRIRLGFKVSQSIPAYIHQIMNDLSKSGDLPKQTTRYPKLDADPNIGPIRYVLIHKALMPESKVSQKGAISLQIKYAIRHLAGSPVKWFGLAPYNPLII",
    "response#3": "LMMGALGVVYGDIGTSPLYTLRACLTEFADLQPAHILGVLSILFWLLMVVVSFKYVLLILRADNQGEGGTLALLELAVRGRSGRLRTFFVVLGIFGAALFYGDSMITPAISVLSAIEGIGVVSHTLDPWIVPLALLVLLALFAIQSRGTGTVGKLFGPVMVVWFATLGVLGGWQVWQTPEVLVALNPIWALRFVFEFPVMSFLLLGAVVLALTGAEALYADMGHFGRPAIRRAWFSMVLPSLTLCYLGQGALLLRDPSAIRNPFFLMAPEWGLAALVGLATVATVVASQAVISGAFSVTRQAVQLGFWPRMQILHTSAVEKGQIYLPQVNALLLCAVLVLVITFRNSENLAAAYGFAVTGTMLMTSILAFAVLPRGSTGAKRLGWMLVLGVLLIIDVLLFSANIFKIHEGGWMPLLVGVVVFTLMMTWRRGRQLLADIQARDRQPLQEFMASVRR"
  },
  {
    "reference": "MQRIIFCEYEKRDTEGLDFVPYPGELGQKIFACIGKVGWAAWLAHQTMLINENRLSPRNPSHRAFLEEELNKFLFERSAAKPEGYIEPDA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit iron ion binding. The designed protein sequence is ",
    "response#1": "MKWCAVGRSESDKCDSWSVASLVQDGTTIDCIKGNTVDDCLKKIMHKEADAMAVDGGQVYTAGKCGLVPAMVEQYDQGQCSAPGAARLYYAVAVIKKGSGVTWENLRNKRSCHTGIGRNAGWNIPMGLIYEQTKNCNFSAFFSSSCAPGADPSSQLCAQCAGNAESINKCKASNEERYYAYAGAFRCLAEGKGDVAFVKHSIVKENTDGQGPEWAKAFLSNDYELICPSKGPVSVENFMSCNLAKVNAHAVVTRPEIRTKVVTFLNNQQSHFGNSASEESFKMFTSPDGENLLFKYSTKCLQEIPAHLDYKGFLGQEYMTVMSSLRR",
    "response#2": "MKWCAVGRSESDKCDSWSVASLVQDGTTIDCIKGNTVDDCLKKIMHKEADAMAVDGGQVYTAGKCGLVPAMVEQYDQGQCSAPGAARLYYAVAVIKKGSGVTWENLRNKRSCHTGIGRNAGWNIPMGLIYEQTKNCNFSAFFSSSCAPGADPSSQLCAQCAGNAESINKCKASNEERYYAYAGAFRCLAEGKGDVAFVKHSIVKENTDGQGPEWAKAFLSNDYELICPSKGPVSVENFMSCNLAKVNAHAVVTRPEIRTKVVTFLNNQQSHFGNSASEESFKMFTSPDGENLLFKYSTKCLQEIPAHLDYKGFLGQEYMTVMSSLRR",
    "response#3": "VKWCVTSTKENLKCTALAAAAPVFSCVARASITDCLTAIKAGEADAITLDGGEIYTAGLDEYKLHPIIAEQYGTSTDTCYYAVAVAKKNTGFGLHQLMGKKSCHTGVGKSAGWNIPIGTLLSMDFIKWKGSDDKKLEEVVGEFFHSSCAPGATDSANLCKLCIGDCSKSSETEPYYNYHGAFQCLKDGKGDVAFVKHLTVPEEEKNDYELLCKDNTRKPIDQFENCDLAKVPSHAVVTRKDNEELAQFIWQSLSSVKNFNLFSSTPYGGKNLMFKDSTTTLVQLPLNVDHTMYLGPHYLESVKALKIVNIPSTTC"
  },
  {
    "reference": "MGIHGITKIITDHAPQAQKTNEIKAYLGRKVAIDASMSLYQFLVADDKNGREWDKARLAKRSDRRAATKESLKEAEEKGNQEEIQKFTRRLVKVTKEHNDDCKKLLRLMGIPVVEAPCEAEAMCAALAKSGKVWAAASEDMDTLCFGAPILLRRLTFSEAKKLPILEFHLDAILKGLELDKNQANLGILLGCDYCDTIKSVGPKTALSLIKKHHNIEEIIKNLTEKQIVPEDFDYQQVRQLFLNPDVTDPKEIDLVWKKPQTEEIVKFMCEQNGFNKDRILKGIEKLEKGIKQSQQGRLDSFFKVSSEPNKNKNKRKNDEKLDKKTKVKSTKAK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions; Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site- terminated flap; It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation.2. The presence of XPG-I, XPG N-terminal domains is critical for the interaction of the designed protein with its intended targets.3. A protein that contributes to DNA replication, removal of RNA primer, base-excision repair by functioning in nucleolus, mitochondrion, nucleoplasm with 5'-3' exonuclease activity, 5'-flap endonuclease activity, DNA binding.4. Ensure the presence of a signal peptide within the protein.5. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.6. The protein design should emphasize Basic and acidic residues compositional bias.7. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MGIHGLMNLVADEAPESCKEQDIKALVGRKVAIDASMALYQFLIAVRSAEHAGAVTQMLTNEAGEVTSHIQGLFNRTIRMLTNGVKPCYVFDGKPPTLKSGELAKRTKARTL",
    "response#2": "MGIKGLTRLLGDNAPRALREQKFESYFGRTVAIDASMHIYQSMVVVGRSGDAMLTDADGQVTSHLLGMFYRTLRMLEAGIKPIYVFEGKPPSMKKGELAARREKREEAAAATRRATLMGIPVVEAPCEAESQCAFLTKYNMAHATATEDADALVFGTKILIRNLNANASSNQNKNTSKNTSKRGYILTEINLEQVLKGLNINRKQFVDLCILLGCDYLDPIPKVGPHTALKLIREYGSLENVFKACKLDESTKHHLKLAKEYGGIPVVEAPCEAESQCAFLTKYNMAHATATEDADALVFGTKILIRNLNANASSNQNKNTSKNTSKRGYILTEINLEQVLKGLNLTMNQFIDFCILCGCKHRIENGIPYVEAPCEAEAQCAELARGGKVFAAASEDMDTLCYEPPYLLRHLTFAEARKMPIDEIQYATALEGLGLDKSTFIDLCILLGCKHRIENHEEFIDLCILLGCDYTSKLPGVGPVRALDGIRQHNRSGTYNNLTNEEGQVTSHIIGMLTRTIRLMESGIKPVYVFDGKPPEMKLLELEQRRAKRQEAQANLQAAMEAGDSEQILKSTKATVKVTKEQNEETKKLLRLMGVPVVDAPSEAEATCAALCRDGKVFAAATEDADCLTFGTKILVRNLMAAESQKKTILEVNLALVLEQLNITMDQFIDFCILCGCDYCDTLKGVGPSTAIKLLMQHGTLEKVLEALGPEKVPANFRFEAARMLQCQNAEE",
    "response#3": "MGIKQLMAIIKDEAPDAVKEGEIKNQFGCVCTFDVDLYRFQKLIPTRSMSIYSFLIAVRSGGEMLTNEEGETTSHLMGMFYRTLRIVDNGIKPVYVFDGAPPKLKSGELAKRFQRKATEGGEDPTPIKKKRGRPRKDEKLASPVSAPSSTPVPAPAPAPVPGLGPVPGLGPVPGLGPAPGLGPAPGLGPAPGLGPAPGLGPAPGLGPAPGLGPAPGLGPAPGLGPAPGLGPAPGLGPAPGLGPAPGLGPAPGLEPAPALAPAPSLAPAPGLAPAPVLAPSLAPAPAPALAPSLAPAPAPALTPSLAPAPAPALTPSLAPAPVLAPSLAPALAPALAPSLAPAPAPAPAPAPAPAPALAPSLAPAPAPAPAPAPAPAPALAPSLAPAPAPAPALAPSLAPAPAPALTPSLAPAPAPAPAPAPALAPSLAPAPAPAPAPAPAPAPALAPSLAPALAPAPAPAPALAPSLAPAPAPALAPSLAPAPAPALAPSLAPAPAPAPAPAPALAPSLAPAPAPALAPSLAPAPAPAPAPAPAPALAPSLAPSLAPSLAPAPAPAPAPAPAPAPALAPSLAPAPAPAPAPAPALAPSLSPARALAPAPAPAPAPAPAPAPAPASAPAPAPAPAPAPAPAPEPAPAPAPAPALAPASAPASASASASASPSPS"
  },
  {
    "reference": "MTSLGVNIDHIANIRQARLANEPDPVQMALLAELGGADGITIHLREDRRHIQDRDLKLLRETIKSRLNLEMAATTEMIEIALDLKPDMITLVPERREEITTEGGLDVASNAKSLKEIVTKMESCCIPTSLFVDANSKQLEASSKIGATWVELHTGPYARASWKNQPLEFAKISEGVARARNLGLRVNAGHGLTYQNVEPIASITEMEELNIGHTIIARAIAIGLKEAVKEMKELIKNPRHEPFFGS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining pyridoxine 5'-phosphate synthase activity.2. The protein should be able to bind 1-deoxy-D-xylulose 5-phosphate, 3-amino-2-oxopropyl phosphate ligand in a variety of conditions3. The protein design should consider the endogenous proteins and enzymes present in the pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 5/5, Cofactor biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MITLLVASKNAGKTKELKQLLAA",
    "response#2": "MNQLVKH",
    "response#3": "MMIKRVIAALIVAAGSGERAGGGPPKQYRRIGGRTVLEHS"
  },
  {
    "reference": "MAAIYEPSAAAETSLDGPAVGVIVVAAGSGTRLGAGIPKAFVSLAGSTVLEHALAPVFRLREPVQLVLVVPADRVGEAETIGRRAAGRASDHLRVVVGGGTRQESVLNGLAALWPGVRTVLVHDAARALTPTAQFERVIEAAARTGWGVVPALPVTDTIKQVDASAVVERTIDRAELRAVQTPQAFPRDALVAAFRVAAERLAAATDDAALYADAGHPVVTVEGSEAAFKITTPWDAQRAERLLTEPAAADALADRADTGLPSSSPSSSPTPQPGRIRTGIGIDVHAFAEAGELHLAGLSWPGERALAGHSDGDAVAHAIVDALLSAAGLGDIGSMFGTDDPRFAGARGSVFLEAAVERLRAAGYEVVNVAVQLVGNRPRFAPRRLEAESVLGALVGGPVSIAATTTDSLGFTGRGEGVTAVATALIERR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. a divalent metal cation is required for the proper folding and stability of the protein.2. We require a protein that can specifically bind to the metabolites produced in the Isoprenoid biosynthesis pathway.3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.4. The designed protein must be optimized for metal ion binding. The designed protein sequence is ",
    "response#1": "MRVGTGFDVHSFVEGDHLWLCGLKIAHSHGLNGHSDSDVGMHALTDAIYGALAQGDIGQHFPPSDMQWKGAPSDIFLRHAVDLAQEMGYRISNCDVTLICERPKIGPHAQAMRARLAQIMGLEVARVSVKATTSEKLGFTGRGEGIAAQASALF",
    "response#2": "MRVGTGFDVHSFVEGDHLWLCGLKIAHSHGLNGHSDSDVGMHALTDAIYGALAQGDIGQHFPPSDMQWKGAPSDIFLRHAVDLAQEMGYRISNCDVTLICERPKIGPHAQAMRARLAQIMGLEVARVSVKATTSEKLGFTGRGEGIAAQASALR",
    "response#3": "MRVGTGFDVHSFVEGDHLWLCGLKIAHSHGLNGHSDSDVGMHALTDAIYGALAQGDIGQHFPPSDMQWKGAPSDIFLRHAVDLAQEMGYRISNCDVTLICERPKIGPHAQAMRARLAQIMGLEVARVSVKATTSEKLGFTGRGEGIAAQASALV"
  },
  {
    "reference": "MGIELFYLLFLFLQRNENVQGNCDPGSAGNCEDCLLSGPHCGWCFQENFTDLSNIYKRCDTPDKLIAKGCPSNLIEFPVSKVVIEKNKFLSEGPQKNNSDVTQISPQKLTVFLRPGNEETIQINVRQTEDYPVDLYYLMDLSASMNDDLKNIKVLGSTLSKEMSKLTSNFQLGFGSFVEKPVSPFINTLAEDIQNPCRGISHDCSPTFGYKHVLPLTNDAERFNDIVKKQKISANIDTPEGGFDAIMQAAVCKEKIGWRNDSLHLLVFVTDADSHFGMDSKLAGIVIPNDGECHLDDNNEYSMSTVLEYPSLGQLIDKIIQNNVLLIFAVTKGQVHLYQNYADLIPGATVGQLQEDSSNVLQLIIDAYKALRSEIELEVLGDTEGINMSFTAICNNNTAIANGKKCSHIKVGEVVTFNLTLSLSACEKSRRKVLIKPVGLSETLEIAIQPECSCTCQKEAEANSSKCNDGNGLFECGVCTCNPGYLGPYCKCDEKESLGPDSCRSSPEQKYCSGRGDCYCGQCVCHLSAYGNIYGPDCECDDFSCVRLRGLLCGGNGDCDCNKCICHNGWMGEYCNCTTAIDNCISEDGTICSERGKCICGQCVCTHPGVSGSSCEKYAAYSDPCISKRNCVECYLMSDDRSQEDCTEICKLVDATVSTEEETSEDKYVPCLLRGENECVITFLMTTDEHGKTTIHSIKQKDCPQHPNTTMIILGVSIAVVVTGVVLLCIWKLFISFHDRKEVAKFEAERLKAKWQTGTNPLYRGSTT",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The ideal protein would be one that plays a critical role in cellular response to ionizing radiation, hard palate development, Langerhans cell differentiation through molecular function activator activity within centrosome, nucleoplasm.2. The presence of Integrin beta subunit tail, Integrin beta subunit VWA domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "AQPVVMATVQLMVQDSLNPTLKNCVLEQTKTPVSCFNVQMCVGATGHNIPQKLHLKAELQLDLQKPRQARRVLLLASRQASLTLSLDLGGRNKPICHTIKAFLRDEADFRDKLSPIVLSLNVSLPPEETGVAPAVVLHGVTHVQEQTRIILN",
    "response#2": "AQPVVMATVQLMVQDSLNPTLKNCVLEQTKTPVSCFNVQMCVGATGHNIPQKLHLKAELQLDLQKPRQARRVLLLASRQASLTLSLDLGGRNKPICHTIKAFLRDEADFRDKLSPIVLSLNVSLPPEETGVAPAVVLHGVTHVQEQTRIILN",
    "response#3": "AQPVVMATVQLMVQDSLNPTLKNCVLEQTKTPVSCFNVQMCVGATGHNIPQKLHLKAELQLDLQKPRQARRVLLLASRQASLTLSLDLGGRNKPICHTIKAFLRDEADFRDKLSPIVLSLNVSLPPEETGVAPAVVLHGVTHVQEQTRIILP"
  },
  {
    "reference": "MASQFHQLRILVWKNWLGVKRQPLWTLVLILWPVIIFIILAITRTKFPPTAKPTCYLAPRNLPSAGFFPFLQTLLCDTDSKCKDTPYGPRDLLRRKGIDGPLFKESEILKKPSNPKRDSNLSLRSTQVPERSHTSLATVPPRPSYDLEGTGTENFNGSQLLTRILGLEKLLKQNSTPEDIRRELCESYPGYTADYAFSWVTLGKNVFNKFCLSNMTLLESSLQELKYQVSQMSSDPDNQKRVFRGLVQVLSFFSQVQQQREVWQLLSSLPDVFQNGTSLSSLFGVLQKANRVLLVVQKVYPRVQTDEGFSTLQKSVKHLLNTLDSPMQGDNSTHAWSDDDEQTLSPSSLAAQLLILENFEDAILNISSNSPYSPYLACVRNMTDNLAKGSPDNLKLLQSTIHFRKSFLQNGSSEDSFPPFLEILKSKLSQLRNLTELLCESETFSSIKKSCQFSNMSFERLCEDHAFHVQLIEAAELGTDLTTGLLYHDNIISAKLRGLLTGDPSKINLNVDWLLEQALQMNYLENITRLIPTVEAMLHVNTSADASEKPGQLREMFKNIDLLKEDLRAIGMSNTSIDKLLAIPIPDNRAEIISRVFWLHSCDTNVTNPKLEDAMKEFCKLPLPERSHQSYLIGLTLLHYLDIYNFTYKVFFPRKDQKPMERMMELFIKLREILNQLASGTHPLLDKMRSLRQMHLPRSVPLTQAMYRNTRMNSPAGSFSTISQALCSQGITTEYLTAMLPSSQKPKGNHTKDFLTYKLTKEEIASKY",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that In the meantime, participates in the transport of the lamellar granules-associated proteolytic enzymes, in turn regulates desquamation and keratinocyte differentiation; Plays pleiotropic roles in regulating glucose stimulated insulin secretion from beta cells, regulating the morphology and fusion of insulin granules, lipid raft abundance and the actin cytoskeleton; Furthermore, is essential for the regulation of cellular cholesterol homeostasis by regulating ABCA1-dependent cholesterol efflux from macrophages through interaction with NR1H2 and ABCA1; Transports lipids such as glucosylceramides from the outer to the inner leaflet of lamellar granules (LGs) membrane, whereby the lipids are finally transported to the keratinocyte periphery via the trans-Golgi network and LGs and released to the apical surface of the granular keratinocytes to form lipid lamellae in the stratum corneum of the epidermis, which is essential for skin barrier function2. The protein should have a high affinity for ATP.3. The protein should contain a Helical membrane-spanning region.4. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.5. The designed protein must have at least one ABC transporter domain. The designed protein sequence is ",
    "response#1": "LLVLVGFGINAATEVSVAKLLKYIIDAIQEGSRADLDWFPLLIVLLVFFRGLGLFMGGYYTAVISRRLIFSIRQEIYAKLIRLPSQYYLDNSSGHISAKIMYNVEQLTAASSESLKIMVKDGLITLGLLGYLLYTNWRLTLCIFIFMPIIGVLVRKASKRMRKLSIQVQNTMGDVNHVVQESIGGQAVVKSFVGEEFEQKRFYKSSEDNLKRGLKMVIVQNLNSPLVQLVMAMAMSLIVWLALRPQILGETTAGEFVAYITAAGLLAKPIKNLTDVNEKLQGHLEISDITFRANEGEVVLVLGNPTSALFKGLFHGHKHLKYSPEGSIRFKDNEYKQFASKCPHQIIYNNEQDIHFPYLTVEQTIDFALSCKFHIPKQERIEMRDELLKEFGLSHVKKTYVGNDYVRGVSGGERKRISIIETFIANGSVYLWDNSTKGLDSATALEFLSITQKMAKATRSVNFVKISQASDKIVSKFDKILMLGDSFQVFYDHVEENEEVVQNDEESASEFKNVGHLEISDITFRANEGEVVLVLGNPTSALFKGLFHGHKHLKYSPEGSIRFKDNEYKQFASKCPHQIIYNNEQDIHFPYLTVEQTIDFALSCKFHIPKQERIEMRDELLKEFGLSHVKKTYVGNDYVRGVSGGERKRISIIETFIANGSVYLWDNSTKGLDSATALEFLSITQKMAKATRSVNFVKISQASDKIVSKFDKILMLGDSFQVFYGTMEECLTHFHDTLQIKKKQSSLIM",
    "response#2": "LLVLVGFGINAATEVSVAKLLKYIIDAIQEGSRADLDWFPLLIVLLVFFRGLGLFMGGYYTAVISRRLIFSIRQEIYAKLIRLPSQYYLDNSSGHISAKIMYNVEQLTAASSESLKIMVKDGLITLGLLGYLLYTNWRLTLCIFIFMPIIGVLVRKASKRMRKLSIQVQNTMGDVNHVVQESIGGQAVVKSFVGEEFEQKRFYKSSEDNLKRGLKMVIVQNLNSPLVQLVMAMAMSLIVWLALRPQILGETTAGEFVAYITAAGLLAKPIKNLTDVNEKLQSAAQSIVSIFDLLDRKSDITFRANEGEVVLVLGNPTSALFKGLFHGHKHLKYSPEGSIRFKDNEYKQFASKCPHQIIYNNEQDIHFPYLTVEQTIDFALSCKFHIPKQERIEMRDELLKEFGLSHVKKTYVGNDYVRGVSGGERKRISIIETFIANGSVYLWDNSVN",
    "response#3": "LLVLVGFGINAATEVSVAKLLKYIIDAIQEGSRADLDWFPLLIVLLVFFRGLGLFMGGYYTAVISRRLIFSIRQEIYAKLIRLPSQYYLDNSSGHISAKIMYNVEQLTAASSESLKIMVKDGLITLGLLGYLLYTNWRLTLCIFIFMPIIGVLVRKASKRMRKLSIQVQNTMGDVNHVVQESIGGQAVVKSFVGEEFEQKRFYKSSEDNLKRGLKMVIVQNLNSPLVQLVMAMAMSLIVWLALRPQILGETTAGEFVAYITAAGLLAKPIKNLTDVNEKLQKGFVDMETMFDLKDFNLTIEPGETVAIVGRSGAGKTSLVNLLVRFQEVTSGSLYLDHIPIQDIELSCLRQQVAMVNQQVVLFNRSVRENIAYGQLEGAAEADIVAAAKAAYAHDFIMNLPQGYDTILGAQGLNLSGGQRQRIAIARAILKNAPILILDEATV"
  },
  {
    "reference": "MRAYWYDNLEGDQRLAHNSGKDLDVEELKKIGVLYYRMPEIESVDALARERDYKNRDEIIVSPEKMGDVYESKVRMFFDEHIHEDEEIRYIRDGKGYFDVRSKGDDWVRIQLEKDDLIILPAGIYHRFTTDEDNYIKAMRLFKEEPKWTPLNRSGEVDGNEYRKAYVAHGAGWILNQRRSWPRRLPAVLMLPEGSLFYQQVVNHRYQTRRVFGGTGHMGRSLVKCALARNDLVTDVGRTFETSPASMERDTENCLGMLCDVRARQSVANVIEKTIERFGRIDVIANCSGYGVIGACEDQDEHEIRNQFETNFMGTLHIIQTSLPYFRSQGAGRYLIFSSTSGALGVPGLGPYCATKYAVEGLIEAMSYEIDSFNVKATLVEPGLVRRDEPDSVTNPLPTWGHFLIKPASEAYAHATSPALHAKRMVQWLGDRQPTSAVKCAELVWQLAHCSYPPLRLVLGSYAIESIRDRLRSVTEELEDWKHLNYPAAPDTTEAMPTTENKNAEREEDIEDMDMEAQGHAT",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Fe(2+) is required for the proper folding and stability of the protein.2. For general function, the protein need meet that Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.3. The Fe(2+), Ni(2+) binding site should be stable and able to withstand changes in environmental conditions.4. The protein with the reaction 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-   methylsulfanyl-2-oxobutanoate + formate + 2 H(+), the Proton acceptor must be optimized to ensure efficient catalysis.5. The protein should be optimized for activity in Amino-acid biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.6. The protein should be optimized for stability and solubility when expressed in nucleus where it will be involved in L-methionine salvage from methylthioadenosine. The designed protein sequence is ",
    "response#1": "MEKRLDFAQEQRKQRQIIESIKAEGFEAYLPREVIIRKETDDGEVHKTEFMLPGYVFVHSLTELPLFRSLVSTRGVTYFFGNGGEPVSLSDREMDRLRIKLESGEELILSPDLFKVGDRIKVIEGPFADFTGVVDEIDAAGRAKVVVNINIFGRPTPVELGFNQVKKIEK",
    "response#2": "MATTALATTAGTTQPAIRPAAGGGTRRPPKFSLGPVLARYAKGDMPMPLFRLGMGVVTLWLMARFFTPVDPNYATFPAILAGLGLLVAQFWPVPSGIMGMLMIAVGTFIGNIETTNAAVSEMFTNIIMVVIFASLMRGVNLRWMIGTILRLSIITILFRLVFLFAGVYAMSQASTQAIFVCAMPTFVMSAVLADQYASQGDVAKVAIAGMLVSNLMSVCVLTVVLAIL",
    "response#3": "MRLTTPHDVDLAIDAAGAGLPVLATCNTVDRLGAALDPAYTTLADAGVTVPRTVLCVSDPAVTDGPELAARYGVAAYPDAEKAVRALTGPVFARMVAGEGPIGGERVHLIGFTEDDTLVSGDAAGAVLVTAGGPAPDAGELRALAADCAALGIPVYAVQLPGPAGVAEASEAGARLVRLPPAEPTRTAGLLLAGLTGDRPDQAGA"
  },
  {
    "reference": "MSVRPCLFVVLAAGKGTRMKSSLPKVLHTIAGQSMLAHVLTTASRIEGGKIAVVAGPGMNAVGAEAVRVVPGARMFEQLAQLGTADAVLAAKPALDEQDGGDVVVLFADTPLVRGEVIARLRAALDSGANIAVLGFRPNNPTGYGRLLIDDHGALTGIREEQDATEAERAIGLCNSGVMAFRVPRLSELLSRIGSANAKGEYYLTDAIEIARQQGLKATVVECDESDVLGVNTREQLAVAEGIWQARARSAFMRDGVTMIAPETVWLNFDTRIASDVSIEPNVIFAAGVTVEEGVQILGFCHIEGATIGKGARIGPFARFRPGARIGANAHIGNFVEVKNVTLGEGAKANHLSYLGDGSVGAGANIGAGTIFCNYDGYNKEKTEIGDGAFIGSNTSLVAPVKIGAGAYIGSGSVITKNVSPGALAIERSAQEERPGWAEKFRAVMSRRKAAQKK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have magnesium ion binding to facilitate regulation of cell shape, peptidoglycan biosynthetic process.2. The protein design should take into account the physiological conditions of the Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway.3. The protein should have a fast turnover rate for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.4. The designed protein should have a high specificity for UDP-N-acetyl-alpha-D-glucosamine binding, with minimal off-target effects.5. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The designed protein sequence is ",
    "response#1": "MAAGQGTRMRSALPKVAHRLAGKPLVRHIIDAALEAGVEDCVVVVGAGATADAVRAAAGTGVRFAVQVEQLGTGHAVACAREAAGGAPYVLIMNGDVPLVLPSTLRRLMAAVSSERAGEPVGLLTVELDDPSGYGRIVRDPDGNVLRIVEQKDASPEELAIREVNTGVMAAPARLLKRWLARLNNDNAQGEYYLTDVIAMAVADGLSVATEQPETEIEVLGVNDRVQQARLERAWQLRQADALMEAGVTLIDPDTTYIGSEVEIGEDTVIEPGVKLSGNTRIGRDVTIGQYSEIHNSQIHEGVTIKQSVISDAIVGEKSKVGPFAQLRPGSELGKETKVGNFVEIKKARLDDGAKVSHLSYIGDAEIGARTNVGCGSITVNYDGANKFKTIIGKDAFIGCNTNLIAPVTVGDGSLIAAGSTITDDVPNESLALARSRGVPARVKRGWKRPVKRPD",
    "response#2": "MAAGQGTRMRSALPKVAHRLAGKPLVRHIIDAALEAGVEDCVVVVGAGATADAVRAAAGTGVRFAVQVEQLGTGHAVACAREAAGGAPYVLIMNGDVPLVLPSTLRRLMAAVSSERATPGLGVIATELADPTGYGRIVREGGRVTAIVEEKDATPEQRRITEINAGIYAFDAAVLRDGLSRVGTDNSQGEEYLTDVLGILREAGHRVGASVAGDHREILGCNDRVQLAAMRRILNARTLERHMRAGVTLIDPDTTYIGSEVEIGEDTVIEPGVKLSGNTRIGRDVTIGQYSEIHNSQIHEGVTIKQSVISDAIVGEKSKVGPFAQLRPGSELGKETKVGNFVEIKKARLDDGAKVSHLSYIGDAEIGARTNVGCGSITVNYDGANKFKTIIGKDAFIGCNTNLIAPVTVGDGSLIAAGSTITDDVPNESLALARSRGVTLLDPMRVDIRGSLKCGQDVIIDVNAVFEGDVALADGVQIGANCVIKNAKIGENTVIAPFSHLEDCEVGANAQIGPFARLRPKAVLADEVHIGNFVEVKNTTIGKGSKANHLTYLGDATIGTKTNIGAGTITCNYDGVNKYQTVIGDEVRIGSDTMLVAPVKIGDKATTGAGSVITKDCEPNKLVVARARQRNVEGWVERKRPGTAAAEAAARALAEKNTDNE",
    "response#3": "MAAGQGTRMRSALPKVAHRLAGKPLVRHIIDAALEAGVEDCVVVVGAGATADAVRAAAGTGVRFAVQVEQLGTGHAVACAREAAGGAPYVLIMNGDVPLVLPSTLRRLMAAVSSERAGGLGLLTMDMADPTGYGRILREGDGVRGIVEHKDATEAQRAVREVNTGILVAPTAALKRWLSALSNDNAQGEYYLTDVIAMAAAEGVPIVSAQPAHAWETVGVNSKAQLAALERHWQQRQAEALLDAGVTLIDPDTTYIGSEVEIGEDTVIEPGVKLSGNTRIGRDVTIGQYSEIHNSQIHEGVTIKQSVISDAIVGEKSKVGPFAQLRPGSELGKETKVGNFVEIKKARLDDGAKVSHLSYIGDAEIGARTNVGCGSITVNYDGANKFKTIIGKDAFIGCNTNLIAPVTVGDGSLIAAGSTITDDVPNESLALARSRGVTLLDPMRVDIRGSLKCGQDVIIDVNAVFEGDVALADGVQIGANCVIKNAKIGENTVIAPFSHLEDCEVGANAQIGPFARLRPKAVLADEVHIGNFVEVKNTTIGKGSKANHLTYLGDATIGTKTNIGAGTITCNYDGVNKYQTVIGDEVRIGSDTMLVAPVKIGDKATTGAGSVITKDCEPNKLVVARARQTLKRDWKRPVKKK"
  },
  {
    "reference": "MRIIYSQVTYSMRGADLLIVIVLLTGTITHIRAGSDECKMRSFGQSSVVCECNATYCDSVGHVSLPPVGHFLSFLSSKAGQRLQREQGQVQKNSTGAALRITLVPTEKYQRVKGFGGAMTDAAAMNILFLSTGAQNQLLRQYFSPEGIEYNMVRVPMASCDFSTRLYTYADSPDDYTLLNFSLAEEDTRMKIPLLQRAQALSARPLSLFATAWSSPAWLKTSGALIGKGSLKGKPGGKEYKTWAQYYIRFLEEYEKHNLSFWGLTTGNEPTAGELTNYSFQALGFTPELQRDWIAMDLGPALHSSPYAKTRLMILDDNRLLLPHWAKVVLSDIHASRYVHGIGVHWYWDHLVPAKISLTATHELYPEYFILGTEACPGWSSLDHGVRLGSWERAEDYAHDIVEDLNNYVTGWTDWNLALDMSGGPNWVKNFVDSPIIVDRTKDVFYKQPTFYSMAHFSKFLWEGSQRIGVSVSEHTSLESCAFVRPDGSVVLIILNRSDAEVGFEVWDQTLGFLPASMPPHSILTLLWATY",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in swimming, cholesterol metabolic process, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "LKGFGGSLSDSAAMNILELSQPAQDNLLSSYFSESGIEYNLLRLPMACSDFSTRPYSYDDVPDDYELKHFRLADEDVKMKIPILQRASAMSKRPLLLYASPWTSPAWMKSNGDVRGKGTLKGQAGDKYHKTWANYFIKFLDEYAKHNVTFWAVTAQNEPLAALFTPSEFPTIAFTAAHQRDFIIHDLGPALAQSSHPTQLIIMDDQRINLPHWAKVVLGNATAARYVAGIGVHWYLDSIVPPGCSLEATYKLFPDHFLLYTEACSGFLTLRFSVSLGCWDRGERYSHSILTVLNHFVAGWTDWNLALDLKGGPNWVKNYVDSPIIVDSSKDVFYKQPMFYHLGHFSKFL",
    "response#2": "NWNVQCWVTGDLKLLICAVEPSRQNPCKNYDLHLFYALLEVAGGSRLAPQKGSFEIVQCNCSSEDRWECRVPVPAAKLHDSFLMYLKITAAWATFWSPLMSVQPA",
    "response#3": "LKGFGGSLSDSAAMNILELSQPAQDNLLSSYFSESGIEYNLLRLPMACSDFSTRPYSYDDVPDDYELKHFRLADEDVKMKIPILQRASAMSKRPLLLYASPWTSPAWMKSNGDVRGKGTLKGQAGDKYHKTWANYFIKFLDEYAKHNVTFWAVTAQNEPLAALFTPSEFPTIAFTAAHQRDFIIHDLGPALAQSSHPTQLIIMDDQRINLPHWAKVVLGNATAARYVAGIGVHWYLDSIVPPGCSLEATYKLFPDHFLLYTEACSGFLTLRFSVSLGCWDRGERYSHSILTVLNHFVAGWTDWNLALDLKGGPNWVKNYVDSPIIVDSSKDVFYKQPMFYHLGHFSKFL"
  },
  {
    "reference": "MNPSESNVKPPLWLLAELTYRCPLQCPYCSNPLDFAQQEKELSTAQWIEVFKQARAMGAVQIGFSGGEPLVRKDLPELIRSARELGFYTNLITSGIGLTQKKIDAFAEAGLDHIQISFQASDETLNAALAGSQKAFQQKLEMARAVKAHGYPMVLNFVLHRHNIDQLDRIIELCIELEADDVELATCQFYGWAQLNREGLLPTRDQLVRAEAVVHRYREKMAASGNLANLLFVTPDYYEERPKGCMGGWGAIFLSVTPEGMALPCHSARQLPIRFPSVLEHSLQDIWFNSFGFNRYRGFDWMPEPCRSCDEKEKDFGGCRCQAFMLTGNADNADPVCSKSEHHGTILAAREQANCTNIQVNQLRFRNRANSERVNSQLIFKG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be designed to have high stability and activity towards [PQQ precursor protein] + S-adenosyl-L-methionine = 5'-   deoxyadenosine + E-Y cross-linked-[PQQ precursor protein] + H(+) + L-   methionine. The designed protein sequence is ",
    "response#1": "LTYRCPLQCPYCSNPLDFARQDKELTTEQWIEVFRQARAMGSVQLGFSGGEPLTRKDLPELIRAARDLGFYTNLITSGIGLTESKLDAFSEAGLDHIQISFQASDEVLNAALAGNKKAFQQKLAMAKAVKARDYPMVLNFVLHRHNIDQLDKIIELK",
    "response#2": "LTYRCPLQCPYCSNPLDFARQDKELTTEQWIEVFRQARAMGSVQLGFSGGEPLTRKDLPELIRAARDLGFYTNLITSGIGLTESKLDAFSEAGLDHIQISFQASDEVLNAALAGNKKAFQQKLAMAKAVKARDYPMVLNFVLHRHNIDQLDKIIELN",
    "response#3": "LTYRCPLQCPYCSNPLDFARQDKELTTEQWIEVFRQARAMGSVQLGFSGGEPLTRKDLPELIRAARDLGFYTNLITSGIGLTESKLDAFSEAGLDHIQISFQASDEVLNAALAGNKKAFQQKLAMAKAVKARDYPMVLNFVLHRHNIDQLDKIIELS"
  },
  {
    "reference": "MGHKTKYIFVTGGVTSSLGKGIISASLAKLLQGRGYSVTIQKLDPYINIDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNIDTSQSNNITTGRIYQNVINKERRGDYLGKTVQVVPHITDEIKRCIQILGNTHDYDFVITEIGGTVGDIESLPYVETVRQLKWEMGTDCIVIHLTLIPYLAAAGELKTKPTQHSVKQLLELGVQPDILVCRTEHELTEELRQKIAKFCNVAPDAVIESIDAETIYDVPILMQNEELDKVVLEKLGLDSSGEAVLTKWNQFLQRHKNPKTTIKIGLVGKYIELKDSYKSIIESFIHAGAHNEVSVDLVWLHSEEINSENVAGHLKPLKGLLVAPGFGERGIEGKIEAIKYARENEIPFLGICLGMQCAVIEFARNVLNLPDAHTTEINPDTKDKVIDIMADQKMIEEKGGTMRLGAYKCAIKPNTLASNIYGQSVISERHRHRYEFNNAYKDRFESHGMISSGINPDTDLVEIVEISAHPWFVGVQFHPEYKSTVANPHPLFIDFVKAAVTVNAEMEKA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The Nucleophile, Nucleophile; for glutamine hydrolysis active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The protein should be optimized for activity in CTP biosynthesis via de novo pathway, CTP from UDP: step 2/2, Pyrimidine metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The protein should have a high affinity for UTP, ATP, L-glutamine, Mg(2+).4. The designed protein must possess CTP synthase activity, ATP binding, metal ion binding to support glutamine metabolic process, 'de novo' CTP biosynthetic process under certain conditions.5. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.6. The designed protein have Glutamine amidotransferase domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "VTIAVVGKYTGMKDAYKSLIEALSHGGIANKVKVNLDWIESEVFEREDPAPFLEHVNGILVPGGFGQRGAEGKIKAVRFARERRVPYFGICFGMQMAVIEAARNLVGIEGANSTEFGETKEPLVGLMTEWLRGNELEKRSKAGDLGGTMRLGAFPAALRPGSRVSKIYGNALEISERHRHRYEVNTAYKDRLEQHGLRFSGMSPDGVLPEIVEYEDHPWFVGVQYHPELKSRPLDPHPLFASFLSDAYLSVMESLKFAGYEINKKIKINWINARKLNKNNVKEQLANAKGILVPGGFGEEGVEGKILAAQYARENNIAYLGICLGMQVACIEFARNVLNLEDANTTELDPNTKNPIIDILHGKNRENIGGTLRLGRYITSIKENTLASELYKGKEAIERHRHRYEFNNDFRKQFEENGIIFSGLYLEKDLVEIIEYSKNDFFIAAQYHPEFTSRPNKPNPLFMGFIDSYKSLNEALYHGGFAHRLKVNIKWIEAEALEEPGGVALLEGADAILVPGGFGDRGTRGMMKAAQVGREREIPYFGICYGFQWATVEYARNVAGLTDADSTECTPDTPTKVIYKLRDLLGVDELGGTMRLGSYACELAPGSLAQKVYGTHLIHERHRHRYEFNCLYERPLAQHGLKITGRSPDGKFAEIAELPGHPWYLAVQFHPEFRSKPTRPHPLFASFVYAADWVTIGVVGKYVGLPDAYKSLHEALVHGGLANRVKVQVRWLDAELFETEENKDYVVAQLEPMHGILVPGGFGERGSEGKIASVQFARERNVPFFGICLGMQMACIEGARNTAGIAEASTTEFGPTAEPVVGLITEWMSKEGLQKREADGDLGGTMRLGAYPAKLAGNSVVASIYDSEDISERHRHRYEVNAHYREPLEKGGLIFSGMSPDGTLPEIVERPDHPWFVGVQFHPELKSKPFDPHPLFASF",
    "response#2": "LQDAYKSILEALIHAGAMNECKVEVRNIHSEYLTKENAKEKLQNLDAVLVAPGFGSRGIEGKIEAIRYARVNKVPFFGICLGMQMACVEFARNVLGMPKAHSTEMDPDTKDGVISMMESQKTITMMGGTMRLGSYACELRPDSTTAEIYGTANITERHRHRYEFNNEYLAAFEEAGFIPVGKNPLTDLVEVVEMKDHPFYIGVQFHPEYKSTVENPHPLFVGFVLNAAASYAKKDAYKSLIEALSHGGIANKVKVNLDWIESEVFEREDPAPFLEHVNGILVPGGFGQRGAEGKIKAVRFARERRVPYFGICFGMQMAVIEAARNLVGIEGANSTEFGETKEPLVGLMTEWLRGNELEKRSKAGDLGGTMRLGAFPAALRPGSRVSKIYGNALEISERHRHRYEVNTAYKDRLEQHGLRFSGMSPDGVLPEIVEYEDHPWFVGVQYHPELKSRPLDPHPLFASFIK",
    "response#3": "LQDAYKSILEALIHAGAMNECKVEVRNIHSEYLTKENAKEKLQNLDAVLVAPGFGSRGIEGKIEAIRYARVNKVPFFGICLGMQMACVEFARNVLGMPKAHSTEMDPDTKDGVISMMESQKTITMMGGTMRLGSYACELRPDSTTAEIYGTANITERHRHRYEFNNEYLAAFEEAGFIPVGKNPLTDLVEVVEMKDHPFYIGVQFHPEYKSTVENPHPLFVGFVVKIALVGKYVELPDAYKSIIEAFIHAGAANECKVKLELIHSERLESENVADLLKGMKGILVAPGFGHRGIEGKIAAVKYARENNIPFFGICLGMQCAVIEYARNILGFGDANSTEMVRHTSHPVIDLMEEQKNIIDKGGTMRLGAYPCNLTQGTKAQLAYGTEEVFERHRHRYEFNNAYLEDFRKAGMVHSGMNPDTGLVEVVEIPTHRWFVGVQFHPEYKSTVVNPHPLFVDFVKAALAHKKDAYKSLIEALSHGGIANKVKVNLDWIESEVFEREDPAPFLEHVNGILVPGGFGQRGAEGKIKAVRFARERRVPYFGICFGMQMAVIEAARNLVGIEGANSTEFGETKEPLVGLMTEWLRGNELEKRSKAGDLGGTMRLGAFPAALRPGSRVSKIYGNALEISERHRHRYEVNTAYKDRLEQHGLRFSGMSPDGVLPEIVEYEDHPWFVGVQYHPELKSRPLDPHPLFASFIQ"
  },
  {
    "reference": "MQGFGREVILGVGGGIAAYKSCDLLRRLRDLGFGVTVVPTPSSLNFVGSATWEALSGRKVTTEVFESVEEVRHISLAKNSNFIIIAPATADLMARIAAGRADDLLTNVILASNSPVLIVPAMHPEMWNDEATKTNVATLRSRGYHVMDPDTGALTSGDVGQGRFPETSRILGEFAEITGSRSDLLGKKVLITAGGTREAIDPVRFIGNQSSGKQGYAVARSAQRRGAQVTLISANSNLADIEGIRTIKVESASQMLSALDAEFTHTDILVMSAAVADARPKQIADAKIKKNEFTNIALVENPDILRTISAKKSRQVIVGFAAETSENLETAQAKMVSKGADILYLNDISGGDIFNSETTHGFILVRGKEPKKVAETTKDTLAHQLLDEALLQLG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should interact with enzymes and substrates within the coenzyme A biosynthesis pathway to enhance or regulate its activity.2. The protein contains novel DNA/pantothenate metabolism flavoprotein C-terminal domains that confer a unique function or activity.3. The designed protein must be optimized for phosphopantothenate--cysteine ligase activity. The designed protein sequence is ",
    "response#1": "KRVLLGVSGGIAAYKTPELVRRLKEQGADVRVVLTQGAKAFVTAMSLQAVSANPVHDNLLDPAAEAAMGHIELAKWADLIVIAPASANLLARLTHGFADDLLTTLCLASTAPIYVAPAMNQQMWAAAATQANMAQLSARGVITIGPDSGEQACGDVGAGRMVQPDAIVAFLS",
    "response#2": "RVLLGISAGIAAYKSAFLARLLVQAGCEVQVVMTEGAQAFITPLTLQALTGRAVRTSLLDPAAELGMGHIELARWADRILIAPATADLMARLAQGQADDLLTTLCLASDAEKLLAPAMNQAMWRNPATQRNVAQLSSDGWTLLGPGQGDQACGDVGPGRMLEPEEIAAVLAAVEAAL",
    "response#3": "RVLLGISAGIAAYKSAFLARLLVQAGCEVQVVMTEGAQAFITPLTLQALTGRAVRTSLLDPAAELGMGHIELARWADRILIAPATADLMARLAQGQADDLLTTLCLASDAEKLLAPAMNQAMWRNPATQRNVAQLSSDGWTLLGPGQGDQACGDVGPGRMLEPEEIAAVEAVAR"
  },
  {
    "reference": "MNVQGKKIVLAVSGGIAAYKACALTSKLKQAGAEVIVMMTEGAKQFVAPLTFQALSRNPVYTDTFDEKVPKQIAHIDVADWADLIIIAPATANIIGKLANGIADDMVTTTLLASKAPVYIAPAMNVHMYAHPAVARNMKQLEEDGYRFIEPGEGYLACGYVGKGRLEEPETIIKVLEQQLTTAQLLLGKKVLVTAGPTREQVDPVRFFTNHSTGKMGYAVAKQAAKLGAEVTLISGPTNLSTPKGVHRINVQSAQEMYEQVMRHFEQQDIIIKSAAVADYRPKQTYDNKMKKTGGPLVMEMERTPDILKTIGEQKTSQYVVGFAAESTDVLHYGEQKLKKKNLDAIVINNIQEQGAGFGGETNKVIYLTAVGERIDFPLQSKEDVALHILQRIALQLKDDRR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A2. The protein design should take into account the physiological conditions of the Cofactor biosynthesis, coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway.3. The protein contains novel Flavoprotein domains that confer a unique function or activity.4. The CTP binding site should be stable and able to withstand changes in environmental conditions.5. The Proton donor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.6. The protein design must be able to perform phosphopantothenoylcysteine decarboxylase activity, metal ion binding in order to contribute to pantothenate catabolic process, coenzyme A biosynthetic process.7. The protein requires Mg(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "KNVIVGVTGGIAAYKAADVVSRLKKQGFNINVIMSKSAQEFINPLTFQSLSQNYVVTDMFDEHKTWEVEHISLAKKADLFLVVPATANVIGKIANGIADDMLTTTIMATKAQVLIAPAMNTNMYENHIVQENIEKLKALGYAFVEPQEGRLACGDVGKGKLAEPKEIVREAMEILARAAKPDLSGKKVVVTAGGTVERIDPVRYISNDSSGKMGFALAKAAHELGADVTLLAANTHLPLPAGIRVVHLESAQEMYDAVLAEWSVTDILVKAAAVADYRAKEVSDRKIKKSGETLTLELVKNIDILESLGKQKTKQLLIGFAAETNDLEKYAMDKLQRKNCDLLIANDVTREGAGFGTDTNLVNLLSDKGEIKELPTSEKLTLSHQILDEVAKILP",
    "response#2": "KKILLGVTGGIAAFKAASLCSKLVQQGAEVRVIMTESATKFITELTLQSLSKNPVYTDTFDEKNPAAIAHINLADWADLVLIAPATANIIGKMAAGIADDMLSTTLLATQAPIMLAPAMNVHMYVHPAVSRNLAELAARGVMLVEPGEGLLACGYTGKGRMEEPESIVAVVELALAAFEGDLFLSGCRILVTAGPTRERLDPVRFITNPSSGKMGYALARMARRRGAEVTLVSGPSALAPPRGVRIVSVTSAMEMHDAVTERFADADVVIKAAAVADYRPAEPSGSKIKKGKETLMLRLERNPDIIAALGKKKGRKILVGFAMETEDLLARARRKMKDKNMDLIVANDLSRAGAGFEGDTNIITLIDRDGEETMPRLPKTEVAEIILDRVVDLLGG",
    "response#3": "KKILLGVTGGIAAFKAASLCSKLVQQGAEVRVIMTESATKFITELTLQSLSKNPVYTDTFDEKNPAAIAHINLADWADLVLIAPATANIIGKMAAGIADDMLSTTLLATQAPIMLAPAMNVHMYVHPAVSRNLAELAARGVMLVEPGEGLLACGYTGKGRMEEPESIVAVVEAAAAALLDPPGTLHGLHVVVSAGPTREYLDPVRFISNPSSGKMGFAVAARARARGARVTLVSGPVNLEVPPGVQFVPVESALEMRDAMLAASESADMVVMTAAVADYRAARPAGEKQAKGSEALTVELTPNPDILAELGSNKGERVLVGFAMETHAGVERAAHKARRKNADFILLNYPTREGTGFGGDDNEVTLIDRAGEPVAEASGSKRAVATAVWEAVVGLL"
  },
  {
    "reference": "MDPIAGRRILVAVCGSIAAVKMPRLVSSLVQQGAQVRCVLSPKAEQFVSPLALASLSRQSCNLEAHQWDPCQPRPLHVALAEWAELVVVAPLSATTLARLVHGMADTLLSSTVLATQVPLLAAPAMNTAMWDAPAVQANWQALCRWPRLLPMAPARGLLACDSRGMGRLPEPELLLQGIRAALATGGVADLKGLRILCSAGPTREFLDSVRCLTNPSSGRMGVAMALMAALRGAAVQLVHGPLSNVPASWLDPLDTCPVVSAAEMGAALAQRLPQVHGLVMAAAVADHAPGITNAVKWPKGALPNPLPLQDTPDLAADLAARTRGDQWMLGFAAEHGLDRARALEKLRRKGLDWIVLNPVGLAGRGFGNQPNGGVLLWADGEECGLASQDKTTMAGAVWSALVGELHQFQRSGPRPILA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for CTP binding.2. The designed protein must possess phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity, metal ion binding to support coenzyme A biosynthetic process under certain conditions.3. The designed protein have DNA/pantothenate metabolism flavoprotein C-terminal domains with specific sequence motifs that are crucial for its activity.4. We need a protein that can perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2 with high efficiency.5. The protein should be optimized for activity in CoA from (R)- pantothenate: step 3/5 pathway and exhibit minimal cross-reactivity with other metabolic pathways.6. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.. The designed protein sequence is ",
    "response#1": "KRILLVIGGGIAAYKSLELIRLLQGRGAAVRCVMTSGAQQFITPLSAAALSGDKVFTELFSLTDEAEMGHIRLSREADLVVVAPATADLMARMAAGLADDLATTVLLATDKPVLIAPAMNTMMWDHAATRRNASQLLADGVAMIGPEAGDLACGEVGSGRMSQPADIVAAIEGLLDPGPALTGRHVVVTAGGTREPLDPVRFLGNRSSGKQGYAFARSALARGARVTLISANVSLGDPAGADLVRVGTTGELREATLKAAAEADVVVMAAAPADFRPATYAPDKIKKSDDGAAPAIELVTNPDIAAELGRRRRPQQVLVVFAAETGDAEANGRAKLARKRADLIVINEVGPDKVFGAETNAATVLGADGTETPVPFGPKEDLADRVWDLVARRLG",
    "response#2": "KNILLGITGGIAAYKTTFLVRLLIKAGASVKVVLTDSASSFVTPLTLATLSKNPVLSSFVNEEDGSVSWNNHVELGLWADLMLIAPATANTLSKMANGICDNLLMATYLSAKCPVYFAPAMDLDMFKHPSTVASFSTLASFGNIMIPATSGELASGLIGEGRMAEPEEMVTFLMQAWRHQGFVAENLTGRHVVVTAGGTREPLDPVRFLGNRSSGKQGYAFARSALARGARVTLISANVSLGDPAGADLVRVGTTGELREATLKAAAEADVVVMAAAPADFRPATYAPDKIKKSDDGAAPAIELVTNPDIAAELGRRRRPQQVLVVFAAETGDAEANGRAKLARKRADLIVINEVGPDKVFGAETNAATLLASGGETRQILQGKKILITAGPTYEAIDPVRFIGNHSTGLMGYELARKAANLGATVFLISGPSHLSIQHSSVSLIKVTTAEEMFNEAHALFPDMDIAVCAAAVADYRPKNMANQKIKKNTEELIIPLIKNKDILFSLGERKEKQFLVGFALETENEVENAIGKLKKKNLDAIVLNSLNDSGAGFGKITNKISELLLDGRTEPLPIASKTALAHALLDWAEANL",
    "response#3": "HVVLGVSGSIAAYKAVDLASKLTQAGVAVHTVMTEAATQLVGPATFRAITGLPVSTSMFDLTNPYSIEHISLSEVADLMLIAPCTANVMAKLATGIADDLLTCTALATRAPILIAPAMHTAMWENAATQANLKALQDRGMSFVGPAVGRLASGGFGAGRFAPVADILGHGRVAEPDEILAAVREQLADGPLAGRHALVTSGPTHEPIDTVRYIANRSSGKQGHAIAAACARLGARVTLVSGPVALDDPAGVDVVRVETARDMLAACESALPADVAVCAAAVADWRVAQAAASKIKKGEGGLPSLAFAENPDILATLSARNDRRPQLVVGFAAETGDIAAYAADKLARKGCDWIVANDVSEGTGTFGGDDNTVHLIAADGVDVWPTLSKDEVAARLVARVAAALEG"
  },
  {
    "reference": "MQESGACRKKPAMPSRNAGSHLVVMKRIQRNGDGAMKRQKHGRRELREVKRTSRRSAKSEDFSGAEDENNGENETEVAVNNSESGEQEGVDDTGMAYNALLTLLGSEKPEKPTKRRKNDEAGVEEINEDDLEIHLTKSDDESDEENEPEVASDDEESDEKFNAFDFHFNQPSEDYMEKASQSVTSKWNNTTKETIDEYVVSTSTPSCMNKESVKLGISSIKKRVRDVYSEKHPSKISGIDASLLDSMLNYKSVLYPYKHYNNTFYRELYSMHAINHVFKTRDNILKNTEKIKHAQEMALEGKSHEDKEYRDQGFTRPKVLILLPTRNAAYEVVENILKYSGSEQQENKKKFKAQFNSNDVPPETKPQDFRDAFKGNNNDFFCLGMKFTRKSAKLYSSFYSSDIIIASPLGLSMILENPDKKKRQYDFLSSIEVFIVDRATQIEAQNWAHVNLVLKYLNKVPKEFHDADFSRIRMWYINDQAPLVRQTLVFCEYTTPPITSLVSSKSVNIGGRVRYKPIYTSKNCIMNSIGLRLKQIFQRFSSSSPMEDSENRFKFFINSMLPSLLSSTSYSDGILVYIPSYFDYVRIKNYMRNSTKFTFEGIDEYSTQSKLTRYRQQFISGKVKILLYTERLHYFRRFEINGVKTLLMYGVPSNPLFYKELVRCIGKSVFKEVADIDLSFVKVMYSQWDAIALERIVGGDRAPVLCSGTNEMYEFR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have a specific function within the nucleolus.2. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "MSEAEKKASQDAQHKEPMADSETQLDSDSAPSSQAEKPAKTKYPLSFWLAFGALCLTGLISAMEGSIVSTSLPSIIAELKAAFQPLYGQLADLWGRRYVMILATAIFLLGSGICGGANSMDMLIWGRAVQGIGAGGINMLVDLIICDLVPMRERGNFIGLLFLFVSIGTTSGPIIGGALTDNTTWRWVFYINLPMGGAALVLLVLFLQVKWKKELSTRDRLKRIDVVGNAILVGATFSILYALTYGGTRYPWSAANIVAPFVLGFVGLGIFIAWESNKRWCPYPVMPLHHFNSRTASASFFISFMTMILAFWVVYFYPVYFQSVLGNTPTISGVHLLPFEVSFPIFAAVGGGLVSKTGRYKPIHMVATSIVTIAIGASSVLTQHTHKAAWAVLQIFIGMGLGSLISTTLQAVQAGLPESETAASTATWAYMRSLGTIWGVSVPAAIFNNRFDQLSGQLDPSIRDNFVRGQAYEHATAQFVQSFEPATREVVIGAYTDALRRVWLVSIAFGAVTVLSTLFEKELTLRTR",
    "response#2": "MSEAEKKASQDAQHKEPMADSETQLDSDSAPSSQAEKPAKTKYPLSFWLAFGALCLTGLISAMEGSIVSTSLPSIIAELKAAFQPLYGQLADLWGRRYVMILATAIFLLGSGICGGANSMDMLIWGRAVQGIGAGGINMLVDLIICDLVPMRERGNFIGLLFLFVSIGTTSGPIIGGALTDNTTWRWVFYINLPMGGAALVLLVLFLQVKWKKELSTRDRLKRIDVVGNAILVGATFSILYALTYGGTRYPWSAANIVAPFVLGFVGLGIFIAWESNKRWCPYPVMPLHHFNSRTASASFFISFMTMILAFWVVYFYPVYFQSVLGNTPTISGVHLLPFEVSFPIFAAVGGGLVSKTGRYKPIHMVATSIVTIAIGASSVLTQHTHKAAWAVLQIFIGMGLGSLISTTLQAVQAGLPESETAASTATWAYMRSLGTIWGVSVPAAIFNNRFDQLSGQLDPSIRDNFVRGQAYEHATAQFVQSFEPATREVVIGAYTDALRRVWLVSIAFGAVTVLSTLFEKELTLRTGRGQ",
    "response#3": "MSEAEKKASQDAQHKEPMADSETQLDSDSAPSSQAEKPAKTKYPLSFWLAFGALCLTGLISAMEGSIVSTSLPSIIAELKAAFQPLYGQLADLWGRRYVMILATAIFLLGSGICGGANSMDMLIWGRAVQGIGAGGINMLVDLIICDLVPMRERGNFIGLLFLFVSIGTTSGPIIGGALTDNTTWRWVFYINLPMGGAALVLLVLFLQVKWKKELSTRDRLKRIDVVGNAILVGATFSILYALTYGGTRYPWSAANIVAPFVLGFVGLGIFIAWESNKRWCPYPVMPLHHFNSRTASASFFISFMTMILAFWVVYFYPVYFQSVLGNTPTISGVHLLPFEVSFPIFAAVGGGLVSKTGRYKPIHMVATSIVTIAIGASSVLTQHTHKAAWAVLQIFIGMGLGSLISTTLQAVQAGLPESETAASTATWAYMRSLGTIWGVSVPAAIFNNRFDQLSGQLDPSIRDNFVRGQAYEHATAQFVQSFEPATREVVIGAYTDALRRVWLVSIAFGAVTVLSTLFEKELTLRTQ"
  },
  {
    "reference": "MFSYSSNPFVYTFIFILSIGNRIAFSSSSSPQAQDPKLVVDEVNRSVFNASRRSLAYLSCRTGNPIDDCWRCDPNWDTNRQRLADCAIGFGKNAIGGRDGRIYVVTDPGNDDPVNPIPGTLRHAVTQEEPLWIIFKRDMVIKLQKELIITSFKTIDGRGASVHITDGPCLKIHEKTNIIIHGINIHDCKPGPGGMIRDGPDHTGMWIPSDGDAVAIFGGKNVWIDHCSLSNCDDGLIDAIHGSTAITISNNHMTHHDKVMLLGHSDSYTEDKNMQVTIAFNHFGEGLVQRMPRFLAPDTRFNKEVTKHEDAPESQWRDWNWRSEGDMLLNGAYFRQSGAGAPSTYARASSLSARPSSLVGSITTTAGALSCRRGGHWLIQQHFRSLPFQKKNKPASPTAATTTDKTVEEILEVELDGVVLTFISASGCANIVFSSAVPARANGHVITHHLDTVRCKLLRDFIIGITLRRAKAISIKLFVDLSAGDLFSEQRGLSVSAANLPLAMNHATAARADPETESSSEEALPVGAEASGPSLVFSSSADDDGDMSGVGAGGDEVEGESDELVGPSDELVGPSDELVGPSELSPELESGLEAGDLGDEDLGGFAPPEAGEALGVVVGFFLAVVGAEAVGGEVGADAVDFGEPVAAGEGEVELLSPLDISAPASGGGSAANTAVTANKATARDNSLKSLNYQIEEKKKMEQLSNGSITDNIFPSAPAPLKQPFVIGVAGGTASGKTTVCDMIMSQLHDQRVVLVNQDSFYHSLSAEK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be optimized for pectate lyase activity, metal ion binding, glycosyltransferase activity, GTP binding activity in order to improve UMP salvage, phosphorylation, CTP salvage.2. The presence of Pectate lyase domains is critical for the interaction of the designed protein with its intended targets.3. The designed protein should interact with enzymes and substrates within the UMP from uridine: step 1/1, Pyrimidine metabolism, UMP biosynthesis via salvage pathway pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "TPQIKGMHTFIRNRETTRDDFIFYSKRLMRLLIEFALSLLPFKDVIVETPQNVNYNGKRINCQKICGVSILRAGETMEQSLCDVLKDVRIGKILIQTNRETDEPELYYLRLPKDIKDYKVMLMDATVASGAAAMMAIRVLLDHDVPEENIFLLSLLMAVSGVHSIAYAFPKVTVVTTQVDPEVSDKFYIIPGIGNYGDRFFGC",
    "response#2": "VVDHPLVAHKLTHLRNEETDSPTFRRLADELVTLLAYEATRDVRVEPIDVVTPVSPTTGVQLAKPRPLVVPILRAGLGMLDGMMRLLPTAEVGFLGMIRNEETLQASTYAERLPEDLSGRQCYVLDPMLATGGTLAAAIRFLTDRGADHITAICLLAAPEGCARLEQELDGLDVPVTIVTAAMDERLNEKGYIVPGLGDAGDRLYVR",
    "response#3": "TPQIKGMHTFIRNRETTRDDFIFYSKRLMRLLIEFALSLLPFKDVIVETPQNVNYNGKRINCQKICGVSILRAGETMEQSLCDVLKDVRIGKILIQTNRETDEPELYYLRLPKDIKDYKVMLMDATVASGAAAMMAIRVLLDHDVPEENIFLLSLLMAVSGVHSIAYAFPKVTVVTTQVDPEVSDKFYIIPGIGNYGDRFFGK"
  },
  {
    "reference": "MTRRCSHCSHNGHNSRTCPNRGVKIFGVRLTDGSIRKSASMGNLSLLSSAAGSTSGGASPADGPDAAPTAADGYASDDFVQGSSSATRDRKKGVPWTEEEHRRFLLGLQKLGKGDWRGISRNFVVSRTPTQVASHAQKYFIRQSNMTRRKRRSSLFDMVPDESMDLPPLPGGQEPETQVLNQPALPPPREEEEVDSMESDTSAVAESSSASAIMPDNLQSTYPVIVPAYFSPFLQFSVPFWQNQKDEDGPVQETHEIVKPVPVHSKSPINVDELVGMSKLSIGESNQETVSTSLSLNLVGGQNRQSAFHANPPTRAQA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Contributes to the sugar-repressed transcription of promoters containing SRS or 5'-TATCCA-3' elements; They may act sequentially and complementarily for adaptation to short- and long-term cold stress..2. The designed protein must possess metal ion binding to support response to sucrose under certain conditions.3. The designed protein should possess a H-T-H motif domain that is capable of recognizing and binding to DNA in a specific orientation and spacing. The designed protein sequence is ",
    "response#1": "FPGTVDHILSEYTRVTTEGGRLSDVLSGYIDPDDGITPPAAEVPPPVDTKTAKADDDSEDEEAEATEDEEEAESGPDPVIAAQRFGAVADQMEVTRKALKKHGRENKQAIAEMLALAELFMPIKLVPKQFEGLVERVRSALDRLRQQERAIMQLCVRDARMPRADFLRQFPGNEVDESWTDALAKGKSKYAEAIARLQPDIIRCQQKLTALK",
    "response#2": "GWKLSKFAREFGMGLTTFKELFGTVYGISPRAWISERRILYAHQLLLNGKMSIVDIAMEAGFSSQSYFTQSYRRRFGCTPSQAV",
    "response#3": "FYQARQRDLYGAGGSNAICADVQHKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKIICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFGH"
  },
  {
    "reference": "MSNSARLPVLSCVEAAAAEAAFIAGDVALSWKLMNLAARGVADEAVSLLGRKPKRILVLAGKGNNGADAFLAALHCARRGTEIVAVFAEGGPARAQAQRAWSARKPGVRIGVVAAANLRLLAAHEFCLIFDGILGQGFHSPLSAELRTFLRASESLRGLRIAVDLPSGLGDDATGPAFRADLTVSIGCLKRPLLAPKSARFVGRLRVLDIGLPLGETEEACVTATSLTPLRRPRRARSDKRHQGRLLVVGGSDRMPGAVLMNTAAALRAGVALVTACLPESVRAKAAVAYPEAMWRGLRTERDGRVASANLKEIRTLLADKDVLLIGSGMGEKSAKLIGAVATRCSADLVLDADALRPSVIAASRQAKVRVLLPHAGEFKRLSGRDASVPAARAYARKTKTIVVLKGPLTCVTDGTRVIHIPFGGPVLARGGSGDLLAGIVASVLARRHELGLTPFDAVVLATTWHARAADWLRETHGEEAVRTTDLLAGLSPVLRG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.2. The protein must have high specificity towards NADHX epimerase activity, ATP binding, metal ion binding, NADPHX epimerase activity. The designed protein sequence is ",
    "response#1": "LLVVAGSEGMTGAAALAGEAAMRVGSGLVSVAVPQSLRTVIEMKLTECLKLGLEESEPGVLGEGAVRQLTPHLHRYHATLLGPGLSTRGEVARVVVALIERAEHPMVLDADALNILAGTLESLEVAKAPLILTPHPGEMARLLRTSTARVQENRLSIAHDFATRHGVVLVLKGARTLIATPEGEVFVNPTGNAGMASGGTGDVLAGMIGGFLVQGHPPRTAALLGVYLHGLAGDLAAEALGERSFIASDLLDRL",
    "response#2": "LLVVAGSEGMTGAAALAGEAAMRVGSGLVSVAVPQSLRTVIEMKLTECLKLGLEESEPGVLGEGAVRQLTPHLHRYHATLLGPGLSTRGEVARVVVALIERAEHPMVLDADALNILAGTLESLEVAKAPLILTPHPGEMARLLRTSTARVQENRLSIAHDFATRHGVVLVLKGARTLIATPEGEVFVNPTGNAGMASGGTGDVLAGMIGGFLVQGHPPRTAALLGVYLHGLAGDLAAEALGERSFIASDLLDRVLMRNACASVAAEIRARWTPRATLVLCGPGNNGGDGLGVAMLLRESGWPVEVALSDSIESFEGDAAIMGHGWGGPFVDFEPNMLDQIVLVVDALFGAGLSRPIEGRVRTMMEGVVARELEVVAVDIPSGVDGDTGQILGFAMCSSVTVTFFRPKPFR",
    "response#3": "PDSHKGTFGHLLVVAGSEGMTGAAALAGEAAMRVGSGLVSVAVPQSLRTVIEMKLTECLKLGLEESEPGVLGEGAVRQLTPHLHRYHATLLGPGLSTRGEVARVVVALIERAEHPMVLDADALNILAGTLESLEVAKAPLILTPHPGEMARLLRTSTARVQENRLSIAHDFATRHGVVLVLKGARTLIATPEGEVFVNPTGNAGMASGGTGDVLAGMIGGFLVQGHPPRTAALLGVYLHGLAGDLAAEALGERSFIASDLLDRLA"
  },
  {
    "reference": "MLRFLRRTLGRRSMRKHAEKERLREAQRAPTHIPAAGDAKSVITCRVSLLDGTDVSVDLPKKAKGQELFDQIMYHLDLIESDYFGLRFMDSAQVAHWLDGTKSIKKQVKIGSPYCLHLRVKFYSSEPNNLREELTRYLFVLQLKQDILSGKLECPFDTAVQLAAYNLQAELGDYDLAEHSPELVSEFRFVPIQTEEMELAIFEKWKEYRGQTPAQAETNYLNKAKWLEMYGVDMHVVKARDGNDYSLGLTPTGVLVFEGETKIGLFFWPKITRLDFKKNKLTLVVVEDDDQGKEQEHTFVFRLDHPKACKHLWKCAVEHHAFFRLRGPVQKSSHRSGFIRLGSRFRYSGKTEYQTTKTNKARRSTSFERRPSKRYSRRTLQMKADAAKPEEPSVHNNVSSQSNGSQQAWSARSAVPVIPSTTSGPVLVEIENLPRSPGTEQHDRRCLPLSINLLESPDLLETAIGGGGGTSDTMETSQTPGEMNTTTRQTGSEEPVVDCGTLKETAEKLKLLEMESSPQLSPRPSVNVNVNSQEEVVKLTEKCLNNAIESSGLNTVRVPPDFKSNILKAQVEAVHKVSREDALSHKNTSIQDASTSSTALNDSNLPLSRDALGLPPAAPADSSSILKEATDELDALLLSLTENLADRTVTPQVSSASTITPRWIIPQSNAISNGLVGCGASLAGKEGAGKKDGLSLISPPAPFSVDAVISSAPPLAEEAALKPRCLLTTEL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should be able to enhance the efficiency of epithelial to mesenchymal transition. The designed protein sequence is ",
    "response#1": "KEQFFHEEECGDLVTRAVLVDMEPKVVSKTLSMAGRSGKWKYDISSQFSQKQGCGNNWANGYCVHGPKHQDIVMDLVRKQVEKCDRLGGFFTIMSMAGGTGSGMGTFLTRCLRDAYPDSLLLNQVIWPYGTGEVIVQNYNSILTLSHLYRSSDALLVHENDIIHKVCSQLMNIKQISFRDVNKVIAHQLGSIFQPVYTSC",
    "response#2": "KEQFFHEEECGDLVTRAVLVDMEPKVVSKTLSMAGRSGKWKYDISSQFSQKQGCGNNWANGYCVHGPKHQDIVMDLVRKQVEKCDRLGGFFTIMSMAGGTGSGMGTFLTRCLRDAYPDSLLLNQVIWPYGTGEVIVQNYNSILTLSHLYRSSDALLVHENDIIHKVCSQLMNIKQISFRDVNKVIAHQLGSIFQPVYTSC",
    "response#3": "KEQFFHEEECGDLVTRAVLVDMEPKVVSKTLSMAGRSGKWKYDISSQFSQKQGCGNNWANGYCVHGPKHQDIVMDLVRKQVEKCDRLGGFFTIMSMAGGTGSGMGTFLTRCLRDAYPDSLLLNQVIWPYGTGEVIVQNYNSILTLSHLYRSSDALLVHENDIIHKVCSQLMNIKQISFRDVNKVIAHQLGSIFQPVYTSC"
  },
  {
    "reference": "MIKLDPIERAIADIREGKAIVVVDDENRENEGDIIFAASKATPELLAFTIRHSSGVVCVPMRGEDLDRLGLPLMVHDNKERLRTAYTISVDARDGVSTGISASDRARTIRTLCDSATEPFELVRPGHVFPLRYREGGVLVRRGHTEAAVDLAVLAGLGSAGVLAEVVNDDGTMMRLPELREFCDLHDLALVSIEQLVEYRRRTERMVTLVAETRLPNQYGMWKAYGYASALDGGEHLALVYGDLEDGENVLVRAHSECLTGDVFGSLRCDCGVQLDRAMSTIAAEGRGVIVYLRGHEGRGIGLLAKLKAYGLQDSGSDTVDANLELGLPVDAREFSNAGQILADLGVKSIRLLTNNPAKVRGMDGYGIKVLGREPMPVAMNPYNEKYLRAKRDRLGHDIPEENA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity.2. The protein should have a high affinity for Mg(2+), GTP.3. The protein should be optimized for activity in riboflavin biosynthesis, Cofactor biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The designed protein must have a Nucleophile; for GTP cyclohydrolase activity active site that is highly conserved among related enzymes.5. The designed protein must be able to perform zinc ion binding, GTP cyclohydrolase II activity under specific conditions. The designed protein sequence is ",
    "response#1": "LATTKISTKYGGEFTVIPFSNIVDDAVHIAFVKGDIKTDDEVLVRVHSQCLTGDVFGSKRCDCGEQLHKAMKMIAKEGKGVLLYMRQEGRGIGLVNKLKAYELQDSGFDTVEANVKLGFKPDLRDYGIGAQILVNLGVRKMRLMTNNPRKIVGLEGYGLSVLKRIG",
    "response#2": "ERIAQAKLPNRLGVWDLFLYKCIFDTEGRDLHVVLTMGGIGVADKNGHIAVQDEPVLVRVHDECLTGDVFGSLRCDCGEQLRKAMEMIAKEGKGVLVYMKQEGRGIGLANKLRAYELQDKEGCDTVEANLRLGFPADLREYGVGAQILHHLGVRKMRLLTNNPRKIVALQGYGLEVVERVDVPMPTRHGAFVAHVFRSKIDGAEHVALTRGMPGPGLDGPRDAVEGPVDCRVHSECLTGDVFGSERCDCEPQLHLALGLLAQADQAVLLYLRQEGRGIGLANKLKAYRLQDAGLDTVEANQALGLPADLREYGIGAQILHYLGVRRMRILTNNPKKIHGLRGYGLELLDQIDVPMPTRHGAFVAHVFRSKIDGAEHVALTRGMPGPGLDGPRDAVEGPVDCRVHSECLTGDVFGSERCDCEPQLHLALGLLAQADQAVLLYLRQEGRGIGLANKLKAYRLQDAGLDTVEANQALGLPADLREYGIGAQILHYLGVRRMRILTNNPKKIHGLRGYGLELLDQILS",
    "response#3": "RTAEAVLPTKYGQFQLVAYESVTDXKTHIALKQGQIVADRPTLVRVHSQCLTGDVFGSLRCDCGEQLDRALELIAEEGEGIVLYMRQEGRGMGLGNKIRAYQLQDQEGLDTVEANEKLGFPADLRDYGIGAQILRDLGXGKMRLLTNNPRKISSLEGHALEVVERVDVPMPTRHGAFVAHVFRSKIDGAEHVALTRGMPGPGLDGPRDAVEGPVDCRVHSECLTGDVFGSERCDCEPQLHLALGLLAQADQAVLLYLRQEGRGIGLANKLKAYRLQDAGLDTVEANQALGLPADLREYGIGAQILHYLGVRRMRILTNNPKKIHGLRGYGLELLDQIAAR"
  },
  {
    "reference": "MKQIILATTNKGKLNDVKEIFNDLDIEILSFLDFENHPEVEENGNTFSDNAIIKARAAFEKYKIPSIGDDSGLEAFQLNGEPGIYSARYAGENADDEKNNLLLIKKLSKLPEPHPGRFVCAAAYYDGKEIKTAIGEIRGNIIKTPRGKNGFGYDPLFIPDGYNITTAEMPHEEKNKISHRYNAFKQLRILL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.2. The designed protein should have XTP diphosphatase activity to facilitate purine nucleoside triphosphate catabolic process.3. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP The designed protein sequence is ",
    "response#1": "SEYIEGTSLNLISNEKYPFEDVAMLLKKIHNVEWKSDNDYNPFKRLEKYEQYCIEKGFVLPKSYLNIRDKFFTYKDYLQSLPKSFAHGDSQPSNFIITDQGLKVVDFEFTGNIDHIYDIACFANMRISDGEQLLKAG",
    "response#2": "MRPPRGRR",
    "response#3": "SEYIEGTSLNLISNEKYPFEDVAMLLKKIHNVEWKSDNDYNPFKRLEKYEQYCIEKGFVLPKSYLNIRDKFFTYKDYLQSLPKSFAHGDSQPSNFIITDQGLKVVDFEFTGNIDHIYDIACFANMRISDGEQLLKAY"
  },
  {
    "reference": "MAAHRSWLLVSFFLLEVLLLEAAKILTISTLSASHYILMNRVSQILQGGGHDVIKLLYEGGDIPDFRKENSSYQVINWRLPEDQQKTFENRWHRLIDEYAYGRSKYHTLLKIHQYFADLCSHLLSRKDIMELLQKENFDLVLLDSMDLCSFLIVEKLGKRFVSFLPFQFSYMDFGLPNAPLSYAPVYGSGLTDQMDFWGRVKNILMFFHFTKKRRDIFSQYGNTVQEHFAEGSQPVLSDLLLKAELWFVNSDFALDFARPLFPNTVYVGGLLDKPVQPIPQDLEDFISQFGDSGFVLVALGSVVSMIQSKEIIKEMNSAFAHLPQGVLWTCKSSHWPKDVSLAPNVKIMDWLPQIDLLAHPSIRLFVTHGGMNSVMEAVHHGVPMVGIPFFGDQPENMVRVEAKNLGVSIQLQTLKAESFLLTMKEVIEDQRYKTAAMASKVIRQSHPLTPAQRLVGWIDHILQTGGAAHLKPYAFQQPWHEQYMLDVFLFLLGLTLGTLWLSVKVLVAVTRYLSISRKVKQA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that contributes to cellular response to genistein by functioning in UDP-N-acetylglucosamine transferase complex with UDP-glycosyltransferase activity, glucuronosyltransferase activity.2. The protein should be designed to catalyze the following reaction glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor   beta-D-glucuronoside + H(+) + UDP.3. Design the protein with a signal peptide incorporated.4. The Extracellular, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.5. For general function, the protein need meet that They are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds .; UDP-glucuronosyltransferases catalyze phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase water solubility and enhance excretion The designed protein sequence is ",
    "response#1": "MPNYESSLKYLRAENLRVINENADIEAFDASKWNLTAETSKELMEKAWIPQNDLLGHPKTKAFITHGGMNGIYEAIYHGVPMVGVPIFGDEDHYSGSTEGWSHWKNRQFNQFKQYLVENCGVASENIQFTKMSSEEIAGAKGLDKSVSKCRVFVANSDHCSEPSVEDVKSYGADLFVHGHSHNKISETYIGGIKCTRIVLGDWRPKGGYLIGIKDGKITLIKHPWIPQNDLLGHPKTRAFITHGGANGIYEAIYHGIPMVGIPLFADGFIEEYIASEINDCKLVALGGFKDMFNDQPIKMEIIQKEYEKRGMT",
    "response#2": "MNKKSWIPQNDLLGHPKTKAFITHGGMNGIYEAIYHGVPMVGVPIFGDGFKDITRIFDEEPKVKLLGPNCPGIISPGKSKIGIMPNHIHQPGKIGIWIPQNDLLGHPKTKAFIAHGGTNGIYEAIYHGIPIVGIPLFGDGFKDITRIFDEQPEVKALGPNCPGIISPGKSKIGIMPNHIHQPGKIGVWISQNDLLGHPKARLLVTHGGVNSIQEAIYHGVPMVAIPLFFDGFKDITRIFNEEPEVKALGPNCPGIISPGKSKIGVMPTHILDPGTIGIASRSGLVSYEMTDTLAEKGYQVTKVVIEGNEACGTTFTEYNMLLKAYKNAVKHMEKELNKK",
    "response#3": "MPTQTDPSSPGRPGRPGRPTIAAAARQAGVSPSTVSRVLSGKGNVSADARRRVLAAVDRLGYRPNALAQGLVSGRTNTIGIVVPELANTVFAETIHSIETALAEHGYTLMLCNSHHDPVREEQHVALLLAKKVDGLLMVSMRLHDPELHVLRDRHIPMVVTMRAVPDPGVGLDHVGMANEQGGYIATRHLLDLGHRRIGMIRGPADTSVGRLRAEGYRAALEDAGLEPRDDLVQEGDFSYESGYRAGAALLERRPDITAIFAANDLMAIGAIGAAQERGLRVPEDLSVVGFDDIPMASWPAYDLTTVRQPIRTMGELAVRLLLDRIEGRGDAPEHIVLPTELIVRDSCAPPR"
  },
  {
    "reference": "MSVTEQFDGVAVATKANVYFDGRCVSHTITLADGTRKSVGVILPASLTFNTGAPEIMEGVAGSCRVRLKGETEWKTWGAGQSFNVPGNSSFDIEVTGEPYHYVCHFG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The desired protein should be able to perform phosphate + xanthosine = alpha-D-ribose 1-phosphate +   xanthine2. The protein must exhibit thymidine phosphorylase activity, pyrimidine-nucleoside phosphorylase activity, guanosine phosphorylase activity, purine-nucleoside phosphorylase activity.3. For general function, the protein need meet that Also catalyzes the reverse reactions.; Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates; Catalyzes the phosphorolysis of diverse nucleosides, yielding D-ribose 1-phosphate and the respective free bases The designed protein sequence is ",
    "response#1": "LVSGHL",
    "response#2": "MXINXEXDXXX",
    "response#3": "LHF"
  },
  {
    "reference": "MVQATLRLESQWPASPAFGWALAPLEVSVLLSDDEAVQALNAQYRGKDKPTNILSFAMEEEADDAMPMPIMAGEPRLLGDLILAYQTVQREAAEQDKPFDQHLTHLLVHGTLHLLGYDHERSEDEAQQQEAREIAILAQLGLPNPYL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be optimized for metalloendopeptidase activity, endoribonuclease activity, zinc ion binding activity in order to improve rRNA processing. The designed protein sequence is ",
    "response#1": "MPW",
    "response#2": "LQII",
    "response#3": "LMXVXPX"
  },
  {
    "reference": "MNLEELVLKYALANAVKYGGKADVKAVMSKIMAEVPELRPKAREVKSMVEAVVARVNSMSLEEQLRLLRERWPETLEERRVEQKRPGIENLAELPNVKGGVVVRFAPNPDFVLHLGSARPAILNYAYRLKYGGKFILRFEDTDPRTKKPLVTSEINAYETIREDLKWLGIKWDEEYIQSMRMEIYYEHIKKLLEKGAAYVDLCRPEEWRKLRNAGRACPHREQSPEENLELWDRMLEGRFKEGEAVVRIKTDLSHPDPSVRDWVAFRIIDTSKTPHPLVGDKYIVWPTYNFAVSIDDHLMGITHVLRAQEHSVNTIKQSYIFKHFGWEQPVTIHFGRLKIEGASLSKSKLKALGVRYDDISLPTLAGLRNRGILPEAIWELILTVGVKPSDSTIALSNLFALNRKRIEPIANRYMYVADPIKLVFKSDRELLAKIPLHPSFRERGERIYKFGPGTIELFVPRQDIKPGAVVRLMELANVEIIAVENGVAHGRLHSVGIDEARKVGAPIIQWVYEPIEIHVVKPVAVGKKVEEIGLGESALEKVETGAYVQFMRYGFLKKVGPSAFVYVHD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Incorporating a 'HIGH' region motif is critical for the successful design of the protein.2. The designed protein should be involved in glutamyl-tRNA aminoacylation by localizing to cytoplasm and exhibiting ATP binding. The designed protein sequence is ",
    "response#1": "IPEEQLTELLKKTIKVHQRDVNKLLEVIQYAWSYYTVLDQSFNYDLLKDNEDFANEDVLAVLKGLKAKLENGDDDLDYSQAIKEVGKDTGIKGRGLYFPLNLAFTGSTSAPQIYEIMDIYSRDTDIL",
    "response#2": "IPEEQLTELLKKTIKVHQRDVNKLLEVIQYAWSYYTVLDQSFNYDLLKDNEDFANEDVLAVLKGLKAKLENGDDDLDYSQAIKEVGKDTGIKGRGLYFPLNLAFTGSTSAPQIYEIMDIYSRDTDILS",
    "response#3": "IPEEQLTELLKKTIKVHQRDVNKLLEVIQYAWSYYTVLDQSFNYDLLKDNEDFANEDVLAVLKGLKAKLENGDDDLDYSQAIKEVGKDTGIKGRGLYFPLNLAFTGSTSAPQIYEIMDIYSRDTDIF"
  },
  {
    "reference": "MKKELLKILREKKPLVHHITNVVTVNDCANITLAIGALPVMAHALEEVEEMVSAADALVLNIGTLTNEQVEAMIKAGKAANRFKVPVILDPVGAGATKLRTQSSKKILEEVKISVIKGNSAEISILAGKGGKIRGVESQGGVDDIAEAAKDLANAYNVVVAMSGATDIITDGKRIAYVKNGHPMMGTITGTGCMLTSVVASFCGVCEDYFETTIEAFVAFGIAGERAAQSSNVKGPGSFKVTFFDEIYNLTPEIIEKDKKVEV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of the hydroxyl group of 4- methyl-5-beta-hydroxyethylthiazole (THZ)..2. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.3. A protein should be able to manipulate the activity of enzymes in the thiamine diphosphate biosynthesis pathway, either by activating or inhibiting their function.4. The protein must exhibit magnesium ion binding, ATP binding. The designed protein sequence is ",
    "response#1": "MRYRL",
    "response#2": "MNGLEYLLLAFKA",
    "response#3": "GFFGH"
  },
  {
    "reference": "MHNPVNVAVVGATGVVGEALLAILHERGFPVAQLRLLASERSAGERVEFAGRKLLVEDLATFDPEGIDVALFSAGSDVAREHAPRFAAAGATVIDNSSAFRDDPQVPLVVAEVNPGAIADRPRGIIANPNCSTMQMLVALAPIHRAAGIERINVATYQSVSGTGRGALEELGRQTAGMLNFREAKCEVYPVQIAFNVIPHGGDFCDNGYTTEEMKLVHETRRILGDDSIGVNATVVRVPVFYGHSEALHIETREKLTARQARELLQDAPGVEVVDEPRGGGYPTAVTHASGHDPVYVGRIREDLSHPRGLSLWVVADNIRKGAALNAVQLAELVLAGRDGTRA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must be able to protein dimerization activity. The designed protein sequence is ",
    "response#1": "QRSHQELISQLLQSYMKLLLPDDEKFHGGWALIDCDPSLIDATHRDVDVLLLLSNSAYYVAYYDDEVDKVNQYQRLSLENLEKIEIGPEPTLFGKPKFSCMRLHYR",
    "response#2": "LNSMGEELERMHPRVYTNISRQLSRAPFGELEDSDMAPMLLNLVAKDLFRSSITWGKIISIFAVCGGFAIDCVRQGHFDYLQCLIDGLAEIIEDDLVYWLIDNGGC",
    "response#3": "QRSHQELISQLLQSYMKLLLPDDEKFHGGWALIDCDPSLIDATHRDVDVLLLLSNSAYYVAYYDDEVDKVNQYQRLSLENLEKIEIGPEPTLFGKPKFSCMRLHYR"
  },
  {
    "reference": "MKITILSGGTGTPKLIQGFKKIIPNKDISVIVNTGEDTYIGDLYLSPDIDTVIYTFSDIINDETWYGLKEDTFFCHEQLKNYGFNEVLKIGDRDRALKMHKTALLKKGIPLSEIVELEKSSLNITSKIYPMSNDLVQSKILIEENGEKLLLKFHDFWIFRKGNANVLDVFYENSNYAKPADGVLRAIEESDFVVIGPSNPITSIGPILSIKEIKEALKEKIVFAVSPIIGENPVSGPTGTLMSAKGYSVDVTGIYGYYKDIVNVMVIDSKDINKKKEIECDVLCIDTIMKTIEDKVNLSKNIVEYYKSKCTY",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be optimized for activity in Cofactor biosynthesis, coenzyme F420 biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The protein must exhibit magnesium ion binding.3. The protein should have a high affinity for 7,8-didemethyl-8-hydroxy-5-deazariboflavin. The designed protein sequence is ",
    "response#1": "LPEVAPGDDLAKLIAAVEPGLVDGDVLLVTSKIVSKAEGRIVRADDREAAIDAETVRVVARRGALRIVENRQGLVMAAAGVDASNTPSGTVLLLPEDPDASARAIRDGLRDTLGVDVGVVVTDTFGRPWRTGLTDVAIGAAGVRVLDDLRGGTDAYGNPLSATVVATADELAAAGDLVKGKAAGLPVAVVRR",
    "response#2": "LPEVRPGDDLAKLIAAIEPTPADGDILLVTSKIVSKAEGRIVEATDREAAIDAETVRVVARRGTLRIVENRQGLVMAAAGVDASNTAPGTVLLLPEDPDASARAIRDGLRDALGVDIGVIVTDTFGRPWRNGLTDVAIGAAGVRVLDDLRGGTDAYGNPLYATVVAVADELAAAGDLVKGKAEGLPVAVVSW",
    "response#3": "LPEVAPGDDLAKLIAAVEPGLVDGDVLLVTSKIVSKAEGRIVRADDREAAIDAETVRVVARRGALRIVENRQGLVMAAAGVDASNTPSGTVLLLPEDPDASARAIRDGLRDTLGVDVGVVVTDTFGRPWRTGLTDVAIGAAGVRVLDDLRGGTDAYGNPLSATVVATADELAAAGDLVKGKAAGLPVAVVRR"
  },
  {
    "reference": "MNQEHLLGMTLSEIQNIILELGLPRFTAKQITDWVYVKRVKSIDEMTNISVRNRDILKKRFDVGRVEPVDVKTSVDGTQKMLFPVKNGKFIESVNIPEEERLTICVSSQVGCKMDCSFCMTGKMGFLGSLSVNEILNQVYSVPNAEALTNLVFMGMGEPLNNFDNVMKAIEVLTADYALAWSPKRITLSTIGVIPNLKRFLEESRCHLAVSLHSPLSVQREKLMPIEKTFKAADIIELLREYDWSHQRRLSFEYIMFDGVNDAPVYARELSKFLSGLDCRVNLIRFHKIPGSNLSPSTEETMIAFRDFLTAKGFTCTIRASRGEDIFAACGMLSTTKEPRTGIQD",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.2. The protein must be able to tRNA binding, rRNA (adenine-C2-)-methyltransferase activity.3. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "KPPVHLADLTPEERIAKIKELGMPGFRAKQLETHYFTHYTSDPAHMTDLPADRREELVAGMLPPLLTEVRRFQTDRGDTIKFLWKLHDGALVESVLMRYPGRITLCVSSQAGCGMNCPFCATGQAGLTRNMSAAEIVEQIVRANRVIADGELGGKKHDDHSAERVSNIVFMGMGEPLANYARVMQAVRVMTAAQPHGLGMSARGITVSTVGLVPAIKKLATEDVPVTFALSLHAPDDLLRDELIPVNSRWKVDEALDAARAYFDATGRRVSIEYALIKDMNDHAWRADLLAEKLNSRGRGWVHVNPIPLNPTPGSIWTASEVPVQNEFVRRLNEAGIPTTLRDTRGKEIDGACGQLVATAEDEART",
    "response#2": "YPHRTTLCLSSQAGCGMACPFCATGQMGLTRNLSTGEMVEQVRVARLACARGDIGDGPTHLTNVVFMGMGEPLANWRHLVGTLHRLVRDVPEGFGMSARNITVSTVGLVPAIDKLSGLGIPVTLAVSLHAPDDDLRDDLIPINSRWKVGELLDAARRYFMATGRRVSIEYALIRDMNDQPWRAQLLADELNKRGRTWAHVNPIPLNPTPGSIWTASTQSAQDEFVDTLRRAGISTTIRDTRGSRD",
    "response#3": "SSQAGCGMACPFCATGQGGLQRNLSTAEIVEQVRLGAAAMRDGILPGGPGRLSNIVFMGMGEPLANFTRVLAAVHRICDPAPGGFGISQRSVTVSTVGLVPAIRKLTEEGMQVRLAVSLHTPDDELRDTLVPVNNRWKVAEVLEAARGYADKTGRRVSIEYALIRDINDHPWL"
  },
  {
    "reference": "MKKAKAIFSLVVALMVLAIFCFAQNTGSTATTAAAAVDSNNDDWLHCKGNKIYDMYGNEVWLTGANWFGFNCSENCFHGAWYDVKTILTSIADRGINLLRIPISTELLYSWMIGKPNPVSSVTASNNPPYHVVNPDFYDPETDDVKNSMEIFDIIMGYCKELGIKVMIDIHSPDANNSGHNYELWYGKETSTCGVVTTKMWIDTLVWLADKYKNDDTIIAFDLKNEPHGKRGYTAEVPKLLAKWDNSTDENNWKYAAETCAKAILEVNPKVLIVIEGVEQYPKTEKGYTYDTPDIWGATGDASPWYSAWWGGNLRGVKDYPIDLGPLNSQIVYSPHDYGPSVYAQPWFEKDFTMQTLLDDYWYDTWAYIHDQGIAPILIGEWGGHMDGGKNQKWMTLLRDYIVQNRIHHTFWCINPNSGDTGGLLGNDWSTWDEAKYALLKPALWQTKDGKFIGLDHKIPLGSKGISLGEYYGTPQASDPPATPTATPTKPAASSTPSFIYGDINSDGNVNSTDLGILKRIIVKNPPASANMDAADVNADGKVNSTDYTVLKRYLLRSIDKLPHTT",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The presence of Dockerin domains is critical for the interaction of the designed protein with its intended targets.2. The ideal protein would be one that plays a critical role in cellulose catabolic process through cellulase activity within cellulosome.3. The designed protein is the optimization of the Proton donor, Nucleophile to achieve high catalytic efficiency for Endohydrolysis of (1->4)-beta-D-glucosidic linkages in   cellulose, lichenin and cereal beta-D-glucans..4. The protein must contain a signal peptide for proper functionality.5. The protein must exhibit the following characteristics: This enzyme catalyzes the endohydrolysis of 1,4-beta- glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.. The designed protein sequence is ",
    "response#1": "PTNDEFFVEAGINASGTNFIEIKAIVNNQSGWPARATNKLKFRYFVDLSELIKAGYSPNQLTLSTNYNQGAKVSGPYVWDSSRNIYYILVDFTGTLIYPGGQDKYKKEVQFRIAAPQNVQWDNSNDYSFQDIKGVSSGSVVKTKYIPLYDEDIKVWGEEPN",
    "response#2": "CDSNGKPIQLKGMSSHGLQWYVNFVNYDSMKFLRDKWGVNVIRAAMYTNEGGYISNPSSQKEKIKKIVQDAIDLNMYVIIDWHILSDNNPNTYKEQAKSFFQEMAEEYGKYSNVIYEICNEPNGGTNWANDIKPYANYIIPAIRAIDPNNIIIVGTSTWSQDVDIAADNPLRYSNIMYTCHFYAGTHTQSLRDKINYAMSKGIAIFVTEWGTSDASGNGGPYLDESQKWVDFMASKNISWTNWALCDKP",
    "response#3": "ITNDEFFVMAGINASGQNFIEIKALLHNQSGWPARVADKLSFRYFVDLTELIEAGYSASDVTITTNYNAGAKVTGLHPWNEAENIYYVNVDFTGTKIYPGGQSAYRKEVQFRIAAPQNTNFWNNDNDYSFRDIKGVTSGNTVKTVYIPVYDDGVLVFGVEPA"
  },
  {
    "reference": "MRVLGIETSCDETGIAVYDEYRGLLVHKVYSQGVLHSRYGGVVPELAARDHIRKVIPLIVGSLKQARLISSDIDAVAYTAGPGLIGALLVGASVACSLAYAWNVPVIGVHHMEAHLLTPMLNKKLIISNSTSFSDFIDVIDFPFIALLVSGGHTQLVMVKNIGEYKILGESVDDAVGEVFDKIAVFLGLGYPGGALLSEMAQVGIKGRYIFPKPMINKPGFNFSFSGLKTAVIRAIMSSSNDQQTRADIACGFERAVVETLSVKCYKALKQMKVKYLVISGGVSANTMLRSKLLKMMYSISGKLLCPELEFCTDNGAMVAYTGLIRLKAGLSNRDLSITVKPRWSLESLPSIF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The substrate binding site should be stable and able to withstand changes in environmental conditions.2. The protein must exhibit the following characteristics: TsaD likely plays a direct catalytic role in this reaction.. The designed protein sequence is ",
    "response#1": "RIDFKEGKLSPEWIHLQNPEAKNYIFTKDGKLRLIATPVTLSDWKSPTFVALRQEHFDMEASAPVVLQKAGVNDEAGISVFMEFHSHYDLFVRQDKDRKRSVGLRYKLGEITHYAKEVSLPTDGEVELVVKSDINYYYFGYKVNGIYHDLGKMNTRYLSTETAGGFTGVVLGLYITSASKDSKAYADFEYFKYLN",
    "response#2": "LLSNVVSSQIKMHEEWGGVYPDLAAREHTKNIIPVLDRALKEASVNIKDIDGIAVTVAPGLIVSLVIGISVAKTLSWIYRKPLIPVHHIEAHIFASFITEKIDYPFIALVVSGGHTELYLIKGFEDYRYLGGTLDDAVGEAYDKVARMLGLGYPGGPVIDRLSKEGEDTVKLPRPLINDRGKNRFNFSFSGLKTAVLREIQKGVYRKEDIARSFQEAATDVLLAKTIDAMKEFNIKNVVIAGGVSANSRLREKFKEAEENHGIKAYFPPLYLCTDNGAMVAL",
    "response#3": "RIDFKEGKLSPEWIHLQNPEAKNYIFTKDGKLRLIATPVTLSDWKSPTFVALRQEHFDMEASAPVVLQKAGVNDEAGISVFMEFHSHYDLFVRQDKDRKRSVGLRYKLGEITHYAKEVSLPTDGEVELVVKSDINYYYFGYKVNGIYHDLGKMNTRYLSTETAGGFTGVVLGLYITSASKDSKAYADFEYFKYR"
  },
  {
    "reference": "MFRKPGLLTSVRRLYSDIPKYNTDFISSSNNAASKSSQSTNNLNSPKTASTSTSDDEIHHFQQLAPTWWDTWGSQRILHKMNLARLDFIQRILRNEIKIENKDVYIPGFNHREHLPAYVSKAIEKDIFKEVNSLLAQKKFDALDIGCGGGILSESLARLPFVQSVTGIDLTPECIEVAKEHALKDPSIKDKIFYKLLPLEKVHGQYDIVTMFEMLEHVNEPSEILSQAWKKLKPDGIMFLSTINRDPISWFTTIFMGEHILKVVPKGTHHLDKYINSSEIEEWFQQNVAEKYQILETKGTMYLPVNGWIEHDCANVGNYFMAIKKVK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway..2. The designed protein should have a binding site that is specific to the target S-adenosyl-L-methionine ligand.3. We require a protein that can specifically bind to the metabolites produced in the ubiquinone biosynthesis, Cofactor biosynthesis pathway.4. The designed protein should be involved in ubiquinone biosynthetic process, methylation by localizing to extrinsic component of mitochondrial inner membrane and exhibiting hexaprenyldihydroxybenzoate methyltransferase activity, 3-demethylubiquinol-6 3-O-methyltransferase activity, 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity.5. The desired protein should be able to perform a 3-demethylubiquinol + S-adenosyl-L-methionine = a ubiquinol   + H(+) + S-adenosyl-L-homocysteine The designed protein sequence is ",
    "response#1": "MDAWFFLCQATSFAFTLGLHRESTYAEYEVEEAEERRRVFWLLFITERGYALQQAKPVMLRSSIHKPQVLCSEDPILAYGEAIALTHLPIRELPGLIRDIDRELCRIYPLGGAEGRPKVAVSAQDELWLLKEARGKLHPET",
    "response#2": "MVNKFAWFFLCQATSFAFTLGLHRESTYAEYEVEEAEERRRVFWLLFITERGYALQQAKPVMLRSSIHKPQVLCSEDPILAYGG",
    "response#3": "MDAWFFLCQATSFAFTLGLHRESTYAEYEVEEAEERRRVFWLLFITERGYALQQAKPVMLRSSIHKPQVLCSEDPILAYGL"
  },
  {
    "reference": "MKKSLIALTLAALPVAATADVTLYGAIKAGVQTYRSVEHTDGKVSKVETGSEIADFGSKIGFKGQEDLGNGLKAVWQLEQGASVAGTNTGWGNKQSFVGLKGGFGTIRAGSLNSPLKNTDANVNAWESGKFTGNVLEISGMAKREHRYLSVRYDSPEFAGFSGSVQYAPKDNSGSNGESYHVGLNYQNSGFFAQYAGLFQRYGEGTKKIEYEHQVYSIPSLFVEKLQVHRLVGGYDNNALYVSVAAQQQDAKLYGARRANSHNSQTEVAATAAYRFGNVTPRVSYAHGFKGTVDSADHDNTYDQVVVGAEYDFSKRTSALVSAGWLQEGKGADKIVSTASAVVLRHKF",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Incorporate a signal peptide in the protein design.2. For general function, the protein need meet that Major antigen on the gonococcal cell surface and it may have pathogenic properties in addition to its porin activity.; Serves as a slightly cation selective porin3. The protein should have a specific function in ion transmembrane transport and should be localized to pore complex, cell outer membrane, endocytic vesicle lumen in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "PQENTFYAGVKAGQASFHDGLRALAREYKVGYHRNSFTYGVFGGYQILNQNNLGLAVELGYDDFGRAKGREKGKTVVKHTNHGTHLSLKGSYEVLEGLDVYGKAGVALVRSDYKLYNENSSTLKKLGEHHRARASGLFAVGAEYAVLPELAVRLEYQWLTRVGKYRPQDKPNTALNYNPWIGSINAGISYRFGQF",
    "response#2": "PQENTFYAGVKAGQASFHDGLRALAREYKVGYHRNSFTYGVFGGYQILNQNNLGLAVELGYDDFGRAKGREKGKTVVKHTNHGTHLSLKGSYEVLEGLDVYGKAGVALVRSDYKLYNENSSTLKKLGEHHRARASGLFAVGAEYAVLPELAVRLEYQWLTRVGKYRPQDKPNTALNYNPWIGSINAGISYRFGQR",
    "response#3": "PQENTFYAGVKAGQASFHDGLRALAREYKVGYHRNSFTYGVFGGYQILNQNNLGLAVELGYDDFGRAKGREKGKTVVKHTNHGTHLSLKGSYEVLEGLDVYGKAGVALVRSDYKLYNENSSTLKKLGEHHRARASGLFAVGAEYAVLPELAVRLEYQWLTRVGKYRPQDKPNTALNYNPWIGSINAGISYRFGQR"
  },
  {
    "reference": "MNKDGMPHEHAAGKGTSNRDWWPNQINLRILHQHPVMANPLGEDFDYTKEFEGLDLKALKKDLTALMTDSQDWWPADYGHYGPFFIRMAWHSAGTYRSGDGRGGANAGAQRFAPLNSWPDNVNLDKARRLLWPIKKKYGRSISWADLLILAGNCAIESMGLPTFGFGGGRRDIWEPEEDIYWGAESEWLGDRRYSGDRDLENPLAAVQMGLIYVNPEGPNGDPDPVASGQDVRETFKRMGMNDEETVALVAGGHTFGKCHGAGDPALVGPEPEAAPIEEQGLGWKSSFGSGRGGDTISSGIEGAWTPHPVKWDMGYLTVLFKYEWELVRSPAGAHQWQAKDVAEEDMVVAAHDPSRKYPPVMTTADLSLRYDPIYAPIAKHYRDNPEEFADAFARAWFKLTHRDMGPRSRYLGPEVPDEDLIWQDPVPAVDHHLIDEKDIEDLKRRILDSGLSISQLVTTAWASASTFRGSDFRGGANGARIRLLPQREWEVNQPGQLKAVIQKLEGIQREFNSSRSDGKKVSLADLIVLGGCAAVEKAAKNAGHEVKVPFTPGRTDASQEQTDPESFEVLEPVADGFRNYSKTRFTVSAEDLLVDRAQLLTLTAPEMTVLVGGLRVLGANHGGSEHGVFTDRLGNLTNDFFVNLLDMGTEWKEKKDGEGVYEGLDRSTGEHRWTGTRVDLIFGSNSQLRAISEVYASDDSEKKFVHDFIAAWDKVMNLDRFDLA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity..2. The protein design should incorporate a binding pocket that specifically recognizes heme b to enhance enzymatic efficiency.3. The designed protein should have catalase activity to facilitate response to oxidative stress.4. The designed protein should have a high specificity for heme b binding, with minimal off-target effects.5. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in AH2 + H2O2 = A + 2 H2O are properly positioned for optimal catalysis.6. The designed protein must have at least one Plant heme peroxidase family profile domain. The designed protein sequence is ",
    "response#1": "AFARAWFKLTHRDMGPLARYLGPLVPKEELLWQDPVPAVTHELISDADIEALKAKILASDVTPAQFIATAWGAAASFRGSDKRGGANGGRIRLEPQKSWEVNQPQVLAKVLTVLEGIQSDFGKPVSIADLVVLAGTAAVEAAAKKAGFDVKVPFTPGRTDASQEQTDVVSFAFLEQKADAFRNYLTPGHKLRAEELMIDRAQLLTLTAPEMAVLLGGLRVLGANHRGSKNGVFTDQPGTLTNDFFVNLLDMATEWKPASTGEGLFEGLDRATGEIKWTATRVDLVFGSNSQLRALAEVYAAR",
    "response#2": "ETMVWQDPIPDVEHDLIGDEEIAELKSEILDSDISRSQLVKTAWASASTYRDSDKRGGANGARIRLEPQKNWEVNEPAKLQSVLATLEDIQAEFNNTRDDDVRVSLADLIVLGGNAAIEEAAAEAGYDVEVPFEPGRADALPEQTDADSFEALKPRVDGFRNYIRDDVDQPAEELMVDDADLLDLTPEEMTVLVGGMRALGATYQDTDLGVFTDQPGTLSNDFFTNLLSMDTKWEQSDSSDQIYEGYDRETGRFKYEGTRLDLIFGSHSRLRAISEVYAAT",
    "response#3": "AFARAWFKLTHRDMGPLARYLGPLVPKEELLWQDPVPAVTHELISDADIEALKAKILASDVTPAQFIATAWGAAASFRGSDKRGGANGGRIRLEPQKSWEVNQPQVLAKVLTVLEGIQSDFGKPVSIADLVVLAGTAAVEAAAKKAGFDVKVPFTPGRTDASQEQTDVVSFAFLEQKADAFRNYLTPGHKLRAEELMIDRAQLLTLTAPEMAVLLGGLRVLGANHRGSKNGVFTDQPGTLTNDFFVNLLDMATEWKPASTGEGLFEGLDRATGEIKWTATRVDLVFGSNSQLRALAEVYAAV"
  },
  {
    "reference": "MSNFSITVIGAGAYGTALAVSFSKKNRKVFLWGRNKKHMQSLKKDRCNKKFLPKINFPNDLKIEISLKKAIKYSNTIVIAVPSIGFKNILIKIQPFLNKNVFLICGTKGLEPRTGRLLQEVVYDILGRETCLSIISGPSFARYLAIGLPTSMVLANNCIKTCKFLANKLKNKFLNIYSISDLVGVQIGGVIKNVIAIASGMSDGIQMGPNAKTAIITYGLEEMYKLGKVMGANEYTFMGMSGVGDLVLTCTDDESRNRKFGILLAQGYSIENAKSKVGCIIEGYNNIKEILILSCKHKINMPIIKQVYKILYMHQPVKKSILNIFLNKK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. We are looking for a protein with broad substrate specificity for NADP(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate   + H(+) + NADPH.2. The designed protein should be involved in glycerophospholipid metabolic process by localizing to glycerol-3-phosphate dehydrogenase complex and exhibiting glycerol-3-phosphate dehydrogenase [NAD(P)+] activity.3. The designed protein should be able to compete with endogenous proteins or inhibitors for NAD(+), substrate binding. The designed protein sequence is ",
    "response#1": "DVIGADVGGAVKNVLAIACGVVAGAKLGNNARAAVISRGFAEMNRFGIALGAKEETLTGLSGLGDLVLTCSSELSRNFTFGKKLGEGYSYEEQQKKRAVTTEGVFTAPVLQRVANKLKVEMPLVSAICDLIEGKQ",
    "response#2": "DIIGGEVASAYKNVIAIAGGICDGLNLGQNAKASLLSRGLVEMCKFGEYFGAKMQTFLGLSGAGDLFLTANSLLSRNYRVGLGLAQNNRLEDILRDLGEVAEGIKTSQAITQISEKEGIYTPIATEIQKIIQGKSPLESL",
    "response#3": "DMIGVQLGGAVKNVIAIGAGISDGMGFGANARIALITRGLAEISRLGSAMGAELSTFMGMTGLGDLVLTCTDNQSRNRRFGILLGKGIDIKEAEKQIGQVVEGYLNTKEVYTLAKRIGVEMPIVEQIYQILYCGKNITEAVSIKEALECEYLVFALSSQGMRAWLKENFINKGQKILIASKGIEDQSCQFLDEIFLDFVPKENFCVLSGPSFAAEVMQKLPTALMISGINQETRIVEDIAGAEVAGAYKNVIAIAAGICEGLGLGKNAAASLISRGLVEMQRFGKVYGAKDESFIGLSGAGDLFLTASSTMSRNFRVGLGIAEGKSKEQIVEELGEVAEGIGTTYALYSIAKEKDLYLPIAREVYYMLESGVWRTG"
  },
  {
    "reference": "MESRGKHRLKKNGESKENLGEHEQARIGFHRNGDSADSLSSPVAEPNFSSPGGRSSNSILHCPPQSPEQGNNLDCLVGEELQFLWPRLQCLQHTLSNLPLSEGLKPLMNFETVEDLAALAGKGLLQELKHDYEILCKSLNSAAPLLSPEGNCESLNYSLQPLIAIVYGPTGSGKSQLLRNLLSCHLIDPSPETVFFVVPQIDMIPPQEMSAWNAQLVEGNYTCGPQQTIVPKSGTLKPRLITLTYDDLTADHNYDVTHPQNIFAQAAQRGPICIIVDECMEELGKHKSIAKFFHAFPSKLHDRFPQCTGYSVFVVLHNMNPRKDQAGNIATLKIQSKCHIISPKMQPSQVARFINTYTKAMPTAITLLLKDIFHHNANHINYDWIIYNTSPEHECMQWMYLHPQNGLMPMYLNVQTILYQLLEKIDKVLRQRQRWNTAYSKKCDKLANK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.2. A protein that can perform DNA binding in host cell nucleoplasm, host cell nucleolus to support DNA-templated transcription.3. The designed protein should have a binding site that is specific to the target ATP ligand.4. For general function, the protein need meet that Component of the DEF-A and DEF-B transcription factors that bind downstream elements of the major late promoter (MLP), and stimulate transcription from the MLP after initiation of viral DNA replication; Component of the packaging machinery which encapsidates the viral DNA into preformed capsids and transcriptional activator of the viral major late promoter (MLP) The designed protein sequence is ",
    "response#1": "EHHIRIPRTPARVTGGVFLVDKNPHNTEESRLVVDFSQFSRGNHRVSWPKFAVPNLQSLTNLLSSNLSWLSLDVSAAFYHLPLHPAAMPHLLVGSSGLSRYVARLSSDSRIFNHQHGTMQNLHDSCSRNLYVSLLLLYQTFGRKLHLYSHPIILGFRKIPMGVGLSPFLLAQFTSAICSVVRRAFPHCLAFSYMDDVVLGAKTVHHLESLFTAVTNFLLSLGIHLNPNKTKRWGYSLHFMGYVI",
    "response#2": "MEKLSEALDLLQEELLSLYEQNSQSLADQSRHWSLLRKEQVLLYYARGKGIMRIGMQPVPPQSVSQAKAKQAIEQSLYIDSLLHSKYANEPWTLCDTSRERLVAEPAYTFKKGGKQIDVRYGDSEENIVRYVLWLDIYYQDEFDTWEKAHGKLDHKGLSYMHGTQQVYYVDFEEEANKYSETGKYEILNQPTTG",
    "response#3": "PIIMGFRKIPMGVGLSPFLLAQFTSAICSVVRRAFPHCLAFSYMDDLVLGAKSVQHLESLYTAVTNFLLSLGIHLNPHKTKRWGYSLHFMGYVR"
  },
  {
    "reference": "MIFNKDFGGFMLENKNILLGVTGGIAAYKIATLASMLKKQKANVKVIMTENAAKFITPMTFETLTANKVYIDTFDRNFEFKVDHIELGKWADVFLIAPASADVTGKLANGIADDMLTTTALAMRCPIVISPAMNTTMYENKIVRHNMMKLRTYGMEVILPDSGHLACGDSGAGKMPQPEMLLEHIKKAVYKEKDLTGKKVCVSAGPTREAIDPVRYISNNSTGKMGVELAKMAAYRGAKVTLVIGPSDVFVPDFITRIDIESAKDMYDEIMKISDSQDIIIKAAAVADYTPAKYSDEKIKKRDTDLSIELSRTKDILKELGKRRADNPKKQFLCGFSMETENMEANSKKKLKSKNVDMIVANNVKVEGAGFGTDTNVVTIFTKDKEIILDKLSKLEVAKKIFDEIVRNF",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. The designed protein should have FMN binding, phosphopantothenoylcysteine decarboxylase activity, phosphopantothenate--cysteine ligase activity, metal ion binding to facilitate pantothenate catabolic process, coenzyme A biosynthetic process.3. A protein that can modulate the overall flux of metabolites through the CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis, Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed.4. The protein have enhanced H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.5. The presence of DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.6. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..7. The CTP binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MRVVLGVSGGIAAYKACSLLRLFTEHGHDVTVVPTAAALQFVGAPTWSALSGKPVSTEVWTSVHEVPHVRIGQAADLVVVAPATADLLAKAAHGLADDLLTNTLLTARCPVAFAPAMHTEMWEHPATQANVATLRSRGAHVIEPAVGRLTGKDSGAGRLPEPEALYALLDRLGAPAVALAGRHVVVSAGGTREPLDPVRFLGNRSSGRQGYALARTAVARGARVTLVAANTALPDPAGADVVRVGTAVQLREAVLKAAADADAVVMAAAVADFRPARYAQGKIKKRDGQEPEPVELVRNPDVLAELSSTRPRPGQVVVGFAAETDDVLVNGRAKLARKGCDLLVVNEVGEEKTFGSTENEAVLLDDAGRRVDVAAGSKDEVADAVWDVVLRVLSDRAA",
    "response#2": "KRILLGITGGIAAYKAAELVRLLVKQGFEVQVAMTEAATQFITPTTMQALSGQPVLLNQWQDEAGMTHIHSSRAADAIVIAPATADFIAKLAHGMADDLLSALCLARDCPLLVAPAMNKQMWSNPATQRNVQQLAADGIAVLGPACGSQACGEEGMGRMLEPAELADEIVAYLAPKLALTGIKLLMTAGPTYEAIDAVRGITNRSSGKMGYAIAQAAREMGAQVTLVSGPVALTTPVGVERISVNSAQEMYDAVNRRAGDADIFIGVAAVADYRVAHPAEQKIKKSAAPMTLELVANPDILADIASLPNPPFCVGFAAESERLAEYAEQKRRAKKLPLLVGNLAQQAIGSDDNELIELIWADGRQHLPRQPKSVLARQLVALIAGRLQARPAA",
    "response#3": "MRIVLGVTGGIAAYKAALVLRLLKEAGHRVRVVPTESALRMVGRPTWEALSGEPVRTSVFDDAEHVDHVALGRGADLVIVAPATANLLAKAATGLADDLLSTTLLTVTCPVLLAPAMHTEMWLHPATQANVATLRGRGVHVLDPDSGRLTGADSGPGRLPEPAAIVAAALGDLLARTRGGGRLAGRHVVVSAGGTREPLDPVRFLGNRSSGRQGYALARTAVARGARVTLVAANTALPDPAGADVVRVGTAVQLREAVLKAAADADAVVMAAAVADFRPARYAQGKIKKRDGQEPEPVELVRNPDVLAELSSTRPRPGQVVVGFAAETDDVLVNGRAKLARKGCDLLVVNEVGEEKTFGSTENEAVVLAADGSVVALEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLLEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLLEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVL"
  },
  {
    "reference": "MIIQRLFGILYMLAGLAKAFPQFENVPAVLRQAAIANQGTWYAAASIWLGAHGDVINILVGVVLFGSGVILMLNPLWTTLVIYAQLLMMAVFVVILHQSQPQVMLLDGVFALAALYMLRGQYHRKPKPRTFPTTSFSLPTPSSESSFSAPLGDEYDVVIIGGGASGLTAASEFTHERVLVLEKSSTFGGNARYHTFNRLKHPTAGVCFQEPFPGSNMLRLLKKIGLEGKYKSNEKDTLVFFDTFLLLKCLGEIVVGFIKQPRYLLKLSVWGLTSQLFLHAIIGKPYVVAAKQLGDPIFADLYTFLDKFSPRGDFYPRLPWTPNGSWSKAHMELLDNISLYTYLFEPDKLGRLPEQLRPPARLGKLVENAVSTTLRVECLDIHDVSAYVGLHFLVGYLRGNLVTLPGGNGSISAGLCKYLSHQRNVTLQNHVQLTAVEPQHNGTCIQFTINGQPRQVQAQQIIWAAPKTQLATWLPGLPAKQLAAIKNIRHEDYYLANVFLSKPVLGHSFGGYMIEPDSNKDPFSWCKAGTCLVANWMDDHADVDVGVLTLLKPTTRSERQDRTAQNAFLALQQQTYAEIAKVLRNIGIGAEVIEDIQIWYWPAGLVTSVVGQQAEGVFETARQSFENIHFANQDSVGVGNIESAILSGIDAANAVKAQLMDTENVVEVAG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must bind to FAD in order to perform its enzymatic function.2. Ideal protein should have a binding affinity for key enzymes in the prodigiosin biosynthesis, Antibiotic biosynthesis pathway to enhance the overall efficiency of the pathway.3. The protein must exhibit the following characteristics: Catalyzes the oxidation of dihydro form of MAP (H2MAP) to yield MAP..4. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "PRILCLHGGGTNARIFRMQCRVLERFLRSTFRFVYAEAPFAAQPGSDVTSVYKDHGPFKAWLRCTAADPDRSAQEVVKKINLSIATAMYDDDMRGATGEWIALLGFSQGAKVAASILYAQQTIQQRLGERAATRPRFRFAVLMAGRGPLVWLLPETSSGPGSIPMGLVDAASPSMLDSEPELPTDSTEHMLRLPTLHVHGLRDPGLSLHRRLLRSYCQSDSVSLVEWEGEHRVPLKTKDVTAL",
    "response#2": "VRASSLRNGVRAPNPRVCLATDYTAYLYDKMMGVGRFHLLLFGSDLQGPVRQRLAVLAGELRKREGFYERFGGREMFNLILVVKTLSHQTAELLEGDLAPLKDHATTVYDDRAPDDDAHYWYGVNHARGALVVVRPDLAVGVSVWPEEIGKLNKYFASFLLD",
    "response#3": "VRASSLRNGVRAPNPRVCLATDYTAYLYDKMMGVGRFHLLLFGSDLQGPVRQRLAVLAGELRKREGFYERFGGREMFNLILVVKTLSHQTAELLEGDLAPLKDHATTVYDDRAPDDDAHYWYGVNHARGALVVVRPDLAVGVSVWPEEIGKLNKYFASFLLN"
  },
  {
    "reference": "LWLWPYLFFIEAVPIRKVQDDTKTLIKTIVTRINDISHTQSVSSKQRVTGLDFIPGLHPVLSLSKMDQTLAIYQQILTSLPSRNVIQISNDLENLRDLLHLLASSKSCPLPQARGLETLASLGGVLEASLLLHRGGSPEQAAGVS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Ensure the presence of a signal peptide within the protein.2. A protein that contributes to negative regulation of glucose import, regulation of brown fat cell differentiation, negative regulation of appetite by leptin-mediated signaling pathway, regulation of natural killer cell activation, phagocytosis by functioning in extracellular space with peptide hormone receptor binding, hormone activity.3. For general function, the protein need meet that Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways ; In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity ; Key player in the regulation of energy balance and body weight control; Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways ; In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses ; Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators The designed protein sequence is ",
    "response#1": "MAAGSLLGLCLGLMASCGTVCPCPSECKCNGNVVQCSDLGLTSIPADLPPNTTTLILNYNEISEIKAGQL",
    "response#2": "MASSSTTSSSRKRGSDDPGSSRPHRGIKLFGFRVCPLSPSSERSKSSSSDSGSSSDSSSSDSGSDSDSGSSSDSSSSDSGSSSDSDSGSSSDSDSGSSSDSDSGSSSDSDSGSSSDSDSGSSSDSDSSSSNSGSSSDSDSSSSDSGSDSDSSSSDSGSSSDSDSGSSSDSDSSSSNSGSSSDSDSSSSDSGSSSDSDSSSSDSGSDSDSSSSDSGSSSDSDSGSSSDSDSSSSDSGSDSDSSSSDSGSSSDSDSSSSDSGSDSDSSSSDSGSSSDSDSSSSDSGSSSDSDSSSSDSGSDSDSSSSDSGSSSDSDSSSSDSGSSSDSDVSSDSGSSSDSDVSSDSGSSSDSDVSSDSGSSSDSDSSSDSDSSSSDSGSSSDSDVSSDSGSSSDSDVSSDSGSSSDSDVSSDSGSSSDSDSGSSSDSDSSSSDSGSDSDSSSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSDSGSSD",
    "response#3": "MEPVDPRLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFITKGLGISYGRKKRRQRRRAPQDSQTHQDSLSKQPSSQPRGDSAGPEESKKKVERETETDPSD"
  },
  {
    "reference": "MAVPKKRTSMSKKRIHKNIWKRKGWRAALKAFALAKSLSTGNSKSFFVRQLNNQTLE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have structural constituent of ribosome within large ribosomal subunit, chloroplast to regulate translation. The designed protein sequence is ",
    "response#1": "MTGNALPLKNIPLGGEVHNVELQPGAGGQLARAAGAVAQIVAKEGNLVTLRLPSGEVRLVSNECWATVGQVGNTDAINMVVGKAGRKRWLGKRPHVRGVVMNPCDHPHGGGEGRSPIGRPRPVSPWG",
    "response#2": "GKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGVCARVAIGQVLLSVRCKDGNANHAQEALRRAKFKFPRRQKIG",
    "response#3": "MTKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGVCARVAIGQVLLSVRCKDGNANHAQEALRRAKFKFPRRQKIG"
  },
  {
    "reference": "MKTTPFTDVHIALGAKMHEFAGFNMPIEYSGIIDEHMTVVNGVGVFDVSHMGEFWVKGPNALAFIQSVTSNDASVLPLGKAQYTCFPNDKGGIVDDLLVYHYEPEKYLLVVNAGNIDKDWEWCVSHNTVGAELENSSDRTAQLAIQGPKAQEVLQRLTPVDLSSIPYYSFVTGEFAGCKNVIISNTGYTGAGGFELYFYPSDGMTIWNAIFEAGKPEGIKPIGLGARDTLRLEMGFCLYGNDLDDTTSPIEAGLGWITKFAEGKNFTNRAELERQKKEGVTRKLCAFELQEKGIPRHGYEIADAEGNVIGVVTSGTMSPVLKKGIGMGYVKPEFAKAGTDIFIKVRNKNLKAQVVKAPFRK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: The glycine cleavage system catalyzes the degradation of glycine..2. The designed protein must be able to regulate glycine decarboxylation via glycine cleavage system.3. The protein should have a low Km value for (6S)-5,6,7,8-tetrahydrofolate + (R)-N(6)-(S(8)-   aminomethyldihydrolipoyl)-L-lysyl-[protein] = (6R)-5,10-methylene-   5,6,7,8-tetrahydrofolate + (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] +   NH4(+). The designed protein sequence is ",
    "response#1": "AIRRYHESLGETDRDVCLIPMSAHGTNPATAMMMGMKVVVVKTDDNGNVDIDDLTAKSEEHSTRLGALMITYPSTHGVFEEGIRKICDLIHKHGGQVYMDGANMNAQVGMMQPADVGADVLHMNLHKTFCIPHGAVPVAGFSLDGRVLPRQHFKLYNSDRQEIGWVCSGTLSPTLQEPVGTCNVVREYLKPGTPILVEVRGSLHTGVIRRLPFYRPGGMVSFAIRRYHESLGETDRDVCLIPMSAHGTNPATAMMMGMKVVVVKTDDNGNVDIDDLTAKSEEHSTRLGALMITYPSTHGVFEEGIRKICDLIHKHGGQVYMDGANMNAQVGMMQPADVGADVLHMNLHKTFCIPHK",
    "response#2": "AIRRYHESLGETDRDVCLIPMSAHGTNPATAMMMGMKVVVVKTDDNGNVDIDDLTAKSEEHSTRLGALMITYPSTHGVFEEGIRKICDLIHKHGGQVYMDGANMNAQVGMMQPADVGADVLHMNLHKTFCIPHGERPIFLDWHKKNAKKVEEFAGWEMPIWYSSIKDEHLAVRNGVAIFDVSHMGEFIFKGKDALEFLQYVTTNDISKPPAISGTYTLVLNERGAVKDETLVFNMGNDTYMMVCDSDAFEKLEAWFNAIKRGIEKFGELDLEIENKTYDMAMFSIQGPKARDLAKDLFDIDINDLWWFQAKEVELDGIKMLLSRSGYTGENGFEVYFEDANPYHPDPERRGEPEKALHVWKTILEAGEKYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLDLYGRHVDLGAKMAGFGGWEMPIEYAGRGVLAEHQAVRGAVGIFDVSHLGKAEVTGAGAAEFVNACLSNDLGRIAPGQAQYTLCCNDEGGVVDDLIAYLFSGERVLLVPNAANNAEVVARLAAAAPAGVSVTDRHTGFGVLAVQGPAAPTLVAALGLPTDGAYMSFVEAAWKGRPVIVCRSGYTGERGYELLPRWDDTPALWDALFAAGEGLGASPVGLGARDTLRTEMGYPLHGQDLSPTITPVQARSGWLQADDSQPLLYSWHEEAGAKIIDFGGYLMPVQYRGIIAEHHAVRSAAGLFDVSHMGNFYVRGARAKEFLQYMTTNDLDNAEDGQAQYNLMLYPHGGIVDDLIIYRIDSETFFLIVNASNAQKDFEWLQQHIAAFEGVVLEDHTDQLSLIALQGPLALNILATVFPALDVPALGAFRFCKVLFQGTEVMIAGTGYTGEKGVEICLPNAMALPLWEALFEAGKESGIQPIGLGARDTLRLEMGYSLYGHEIDQDTNPLEARLKW",
    "response#3": "LYGRHVDLGAKMAGFGGWEMPIEYAGRGVLAEHQAVRGAVGIFDVSHLGKAEVTGAGAAEFVNACLSNDLGRIAPGQAQYTLCCNDEGGVVDDLIAYLFSGERVLLVPNAANNAEVVARLAAAAPAGVSVTDRHTGFGVLAVQGPAAPTLVAALGLPTDGAYMSFVEAAWKGRPVIVCRSGYTGERGYELLPRWDDTPALWDALFAAGEGLGASPVGLGARDTLRTEMGYPLHGQDLSPTITPVQARSGWVAGFSLDGRVLPRQHFKLYNSDRQEIGWVCSGTLSPTLQEPVGTCNVVREYLKPGTPILVEVRGSLHTGVIRRLPFLDWHKKNAKKVEEFAGWEMPIWYSSIKDEHLAVRNGVAIFDVSHMGEFIFKGKDALEFLQYVTTNDISKPPAISGTYTLVLNERGAVKDETLVFNMGNDTYMMVCDSDAFEKLEAWFNAIKRGIEKFGELDLEIENKTYDMAMFSIQGPKARDLAKDLFDIDINDLWWFQAKEVELDGIKMLLSRSGYTGENGFEVYFEDANPYHPDPERRGEPEKALHVWKTILEAGEKYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLDDWHKKNAKKVEEFAGWEMPIWYSSIKDEHLAVRNGVAIFDVSHMGEFIFKGKDALEFLQYVTTNDISKPPAISGTYTLVLNERGAVKDETLVFNMGNDTYMMVCDSDAFEKLEAWFNAIKRGIEKFGELDLEIENKTYDMAMFSIQGPKARDLAKDLFDIDINDLWWFQAKEVELDGIKMLLSRSGYTGENGFEVYFEDANPYHPDPERRGEPEKALHVWKTILEAGEKYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLD"
  },
  {
    "reference": "MGGKWSKSSGWATIRERMRRAEPAEPAADGVGAVSRDLEKHGAITSSNTNSAACAWLEAQEEEEVGFPVKPQVPLRPMTYKAALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVDPDKVEEANEGENNSLLHPMSQHGMDDPEKEVLVWKFDSRLAFHHMARELHPEYYKDC",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should include a Dileucine internalization motif; necessary for CD4 internalization, Diacidic; necessary for CD4 internalization, PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif to ensure proper function.2. For general function, the protein need meet that Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis3. The designed protein should be involved in suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host autophagy, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II by localizing to membrane and exhibiting SH3 domain binding. The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPAIRERMRRAKPAAEGVGAVSQDLDKHGAVTSSNINNPASCAWVEAQEEEGVGFPVRPQVPLRPMTFKGALDLSHFLKEKGGLDGLIYSQKRQDILDLWVYNTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVDPDKVEEANEGENNSLLHPMSLHGMEDPEKEVLVWKFDSRLAFHHMARELHPEFYKDC",
    "response#2": "MGGKWSKSSIVGWPAVRERMRRAEPAEPAAEGVGAASRDLEKYGALTSSNTATNNAACAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSRKRQEILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPEKVEEATEGENNSLLHPMSLHGMEDPEGEVLQWKFDSRLAFHHMARELHPEYYKNC",
    "response#3": "MGGKWSKRSGGGWSAVRERMRRAEPAAEGVGAASRDLEKYGALTSSNTAANNADCAWLEAQEDEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRYPLTFGWCFKLVPVDPEKIEEANEGENNCLLHPMSQHGMEDEDREVLKWKFDSRLAFHHVARELHPEYYKDC"
  },
  {
    "reference": "LFECSFSCEQEKEGDKPCKKKKCKGGWKCKFNMCVKV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein localizes to the extracellular region, host cell postsynaptic membrane. The designed protein sequence is ",
    "response#1": "MFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVLPCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVL",
    "response#2": "KEEMKNCSFNVTTLLRDKKEKKEALFYRLDIVPLNGKNSSKNSSEYYRLINCNTTAMTQACPKVTFDPIPIHYCTPAGYAILKCNEKNFTGIGKCSNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIISSXNLTDNGKIIIVRLNQSVEIVCTRPNNNTRKGIRIGPGQTFYATGDIIGDIRLAHCNISKDDWNKTLQKVSKKLAEHFPNKRINFTSSSGGDLEIVTHSFNCRGEFFYCNTSGLFNFTYMPNSTDMPLNDTTNNSRSVITLPCRIKQVINMWQEVGRAMYAPPIEGNITCKSNITGLLLFRDGGKGENTTEIFRPGGGDMKDNWRSELYYYKVVEIKPLYCIAPTTAERRKVVEREKR",
    "response#3": "DIDVGQAHRNESFYQCCSGFCIDLLEKFAEELGFTYELVRVEDGKWGTLENGKWNGLIADLVNRKTDMVLTSLMINTEREAVVDFSEPFMETGIAIVVAKRTGIISPTAFLEPFDTASWMLVGIVAIQAATFMIFLFEWLSPSGYDMKLYLQNTNVTPYRFSLFRTYWLVG"
  },
  {
    "reference": "MVWDRQTKMEYEWKPDEQGLQQILQLLKESQSPDTTIQRTVQQKLEQLNQYPDFNNYLIFVLTKLKSEDEPTRSLSGLILKNNVKAHFQNFPNGVTDFIKSECLNNIGDSSPLIRATVGILITTIASKGELQNWPDLLPKLCSLLDSEDYNTCEGAFGALQKICEDSAEILDSDVLDRPLNIMIPKFLQFFKHSSPKIRSHAVACVNQFIISRTQALMLHIDSFIENLFALAGDEEPEVRKNVCRALVMLLEVRMDRLLPHMHNIVEYMLQRTQDQDENVALEACEFWLTLAEQPICKDVLIRHLPKLIPVLVNGMKYSDIDIILLKGDVEEDETIPDSEQDIRPRFHRSRTVAQQHDEDGIEEEEDDDDEIDDDDTISDWNLRKCSAAALDVLANVYRDELLPHILPLLKELLFHHEWVVKESGILVLGAIAEGCMQGMIPYLPELIPHLIQCLSDKKALVRSITCWTLSRYAHWVVSQPPDTYLKPLMTELLKRILDSNKRVQEAACSAFATLEEEACTELVPYLAYILDTLVFAFSKYQHKNLLILYDAIGTLADSVGHHLNKPEYIQMLMPPLIQKWNMLKDEDKDLFPLLECLSSVATALQSGFLPYCEPVYQRCVNLVQKTLAQAMLNNAQPDQYEAPDKDFMIVALDLLSGLAEGLGGNIEQLVARSNILTLMYQCMQDKMPEVRQSSFALLGDLTKACFQHVKPCIADFMPILGTNLNPEFISVCNNATWAIGEISIQMGIEMQPYIPMVLHQLVEIINR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Involved in nuclear import of M9-containing proteins; In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19; Serves as receptor for nuclear localization signals (NLS) in cargo substrates; In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import; Functions in nuclear protein import as nuclear transport receptor; May mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism; Involved in hnRNP A1/A2 nuclear export; Mediates nuclear import of ADAR/ADAR1 in a RanGTP-dependent manner .; The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.2. The protein can be expressed in nucleus and perform nuclear localization sequence binding, nuclear import signal receptor activity, small GTPase binding without triggering undesirable cellular responses.3. The designed protein must have at least one Importin N-terminal domain. The designed protein sequence is ",
    "response#1": "MADNEKLDNQRLKNFKNKGRDLETMRRQRNEVVVELRKNKRDEHLLKRRNVPQEDICLGIFKRWRPLFRSDALFLEIKYVLDRFCEPFLALFVQTNNLLRNGPQDAESLNSLFQVILLECKLFYDLNCQDIPEFFEDHMSEFMTAFLNYFTYTNPSLEGDEGETNVLIKVKASICEIVELYTLRYEEVFTMLYDFVNVTWTLLTTLTPDEKYDGLVGKAMAFLTSVIRIRKHAEFFQQDQVLQQFIELVVLPNICLRESDEELFEDDPLEYVRRDLEGSNSDSRARSAIVLVRGLLDHFDQKITSVVSTHINANLQQFSTNPSLEWNKKYVALQLFSAIAIKGQSTRLGVTSINLMVDVVAFFENNIKPDLLQPAGVIHPMVLAEDIKYVFTFRNQLNSQQLIDIFPTILRFLEMPSFVVYTYAAIAEDAPMPEVWEDTPPKVEFKQVVEDDAIYSKRCKVFIKKDKDFGDRGVGTLYLKPVKDSEKIQLLVRADTNLGNILVNLILSKGIPCQRMGKNNVLMVCVPTPEDSKATSLLLRVKTGDEADDLLEKIKEHFGM",
    "response#2": "RMKSYKNNALNPEEMRRRREEEGIQLRKQKREQQLFKRRNVELINEEAAMFDSLLMDSYVSSTTGESVITREMVEMLFSDDPDLQLATTSNLLEACDDNSLIFKRWRPLFRSDALFLEIKYVLDRFCEPFLALFVQTNNLLRNGPQDAESLNSLFQVILLECKLFYDLNCQDIPEFFEDHMSEFMTAFLNYFTYTNPSLEGDEGETNVLIKVKASICEIVELYTLRYEEVFTMLYDFVNVTWTLLTTLTPDEKYDGLVGKAMAFLTSVIRIRKHAEFFQQDQVLQQFIELVVLPNICLRESDEELFEDDPLEYVRRDLEGSNSDSRARSAIVLVRGLLDHFDQKITSVVSTHINANLQQFSTNPSLEWNKKYVALQLFSAIAIKGQSTRLGVTSINLMVDVVAFFENNIKPDLLQPAGVIHPMVLAEDIKYVFTFRNQLNSQQLIDIFPTILRFLEMPSFVVYTYAAIAQLCRRAK",
    "response#3": "RMKSYKNNALNPEEMRRRREEEGIQLRKQKREQQLFKRRNVELINEEAAMFDSLLMDSYVSSTTGESVITREMVEMLFSDDSDLQLATTGPNPYCGLIEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGVEDDDSSLAPETPEDTPPKVEFKQVVEDDAIYSKRCKVFIKKDKDFGDRGVGTLYLKPVKDSEKIQLLVRADTNLGNILVNLILSKGIPCQRMGKNNVLMVCVPTPEDSKATSLLLRVKTGDEADDLLEKIKEHL"
  },
  {
    "reference": "MSINAVDSKKLVEALLGDLRLLSQEAKKKQNHVKEAAESGVVRIRNISTASVGDTVLITNLRAACTELLHPLVLSCETRHTRLVQIALQGIQRLVQHRILSQNGATIVTNELWSLVEAECEELRVLQTVPPLVSSELIVTGNTLAKCIVMCFRLHFAKDPIVINAASAAVRQLVSTVFERVIQEDGIFSTELTVVNPSGGRPSPRAAPPTLRPCAADAYMLFKDLCLLINGEAPIWLVGIQETTRTLGLELLESLLKGFPSVFIRHTEFGDLLKDDVCPLIIRLFSPNVKAMHISSQHPSSRTSNASFNNYPPTISHERQSFPISMRLVRIVTLIVQFYQTILHTECEIFISTLLKFVDGDRKGWQRPLALESLHRIVSSTDLVKWMTESFDCRPNSTHVLEQVAIGLSTVVQQCLVCTTFSSDQENEIDRSQEDGGPGFLSKGLWVPYVEHLTSKKTILLDSLDRMDAVAIPEGYVLSRCCVALCDMTQAVYAAIDKLCLVDENSEAGSSETKLEIAKVAYANSQPSILAAIGSLLAASTDEIVSDQLLCCLSTLISAGCRVGADADLHRSVYVLAIMSLPSPSYLNQFAGIAPPSPANKRDAPVSEQVFDLEAWPSTTQVTASGPPCPCPVVSTELWNKQVLLTSKNLQAARTFIASITTHIKELNNLWYLCMATCEHLSWLLAMRPTQVGQFERETRDDHSNVPTVVTNAALSDIAMLSSLMDKVAPAIAALPDKSFLSVIDALIRLSDESLAVAATGRESSLFP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein localizes to the Golgi apparatus.2. The protein must exhibit the following characteristics: May be required for traffic between late Golgi and early endosomes.. The designed protein sequence is ",
    "response#1": "VPWRTADCRYVNNEIYVDLVETVNATLRQKGSSLTLINGSLSGKIDVKCYLSGNPTVQLKLRTSGHPLDNSALHRCVELGEAGVATMNFVPPDGRFTLAEYAIDLSAISQAARRLTNLGLVTVSLASGLGQHRDEFEIKVIIGNSTQVAAIEDLRITVYFPDISDAAKIKILRTTHGGWESDLSRVRGVWAFDKQTAVGSVPVLRGCVENPESTPHAPPVFPSHLAVSYSHVGQLPSGIRVDTIALSDLPPGSKPFKGVKYTSRAGDYIVR",
    "response#2": "VPWRTADCRYVNNEIYVDLVETVNATLRQKGSSLTLINGSLSGKIDVKCYLSGNPTVQLKLRTSGHPLDNSALHRCVELGEAGVATMNFVPPDGRFTLAEYAIDLSAISQAARRLTNLGLVTVSLASGLGQHRDEFEIKVIIGNSTQVAAIEDLRITVYFPDISDAAKIKILRTTHGGWESDLSRVRGVWAFDKQTAVGSVPVLRGCVENPESTPHAPPVFPSHLAVSYSHVGQLPSGIRVDTIALSDLPPGSKPFKGVKYTSRAGDYIVE",
    "response#3": "VPWRTADCRYVNNEIYVDLVETVNATLRQKGSSLTLINGSLSGKIDVKCYLSGNPTVQLKLRTSGHPLDNSALHRCVELGEAGVATMNFVPPDGRFTLAEYAIDLSAISQAARRLTNLGLVTVSLASGLGQHRDEFEIKVIIGNSTQVAAIEDLRITVYFPDISDAAKIKILRTTHGGWESDLSRVRGVWAFDKQTAVGSVPVLRGCVENPESTPHAPPVFPSHLAVSYSHVGQLPSGIRVDTIALSDLPPGSKPFKGVKYTSRAGDYIVA"
  },
  {
    "reference": "MTDEIKTDKTVSFGFEEVSPEEKVSRVKGVFQSVAGRYDLMNDLMSAGVHRLWKHDTIAKLNPQAGEHLLDVAGGTGDLAKAFIDRADRAGRRRRRDSQAQATVCDINEAMLNAGRARRDMAAYEGRLDWVCGDAQALPFEDKTFDALSIAFGIRNVADRAAALSEFRRVLKPGGRLAVLEFSHMTAPAFQKLYDAYSFSVIPRLGEVIASDRESYQYLVESIRRFPKQEAFRKELDTAGFAAVSVTNFSGGIAALHFGWAA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be designed to catalyze the following reaction a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine.2. The designed protein should be optimized for S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity activity in order to improve methylation. The designed protein sequence is ",
    "response#1": "MFEKLPNVFPK",
    "response#2": "MTDPIADMLTRIRNGQAANKVSVEMPSSKLKV",
    "response#3": "MIHDKNTKPE"
  },
  {
    "reference": "MKITSLHFLIFHKNLNYVSSKVKAKKIFYQRALNNIYLCTIRTMIVDIAEIKKRDNHALDTQESQELVNKIKEIKNSDEQNNSNNNNNNSSSSNFCSNNNSPFSHKETKLMVKEEVPNSIVKNILNNNSCATSIINNKFYTQINNIIPVKPEAMKEENINVSTVNTENDISKEKKENSHYFCDEIKVMKKEYSKDDFVTDVKLEMNDKEEEEEEKQKIGQESTHINIKVEKDTFNECNNSNVNEKKRNRSVDIHNELSNKRILTEDVVVKCNIKNDVKIIVTWNMNSITVRYKNKKKWDEFMNFFNNLNADVLCFQEVRLPAMNLSEPCDNKNKNKNKNDGIRDRGKIKNSDQKSLADYEIMEQILNDDFKDYNAYFSLANIKYSGQLVLVKKNIHIESIRYNLFFENNAHIHHDEGRVILVEFSNFFLLSTYTPNNGFDHVKFERRRLFDEQLQKFVTILRNEKQKPLVWTGDLNIAPEDIDLSHPAEFRRMKKGNVPKEFIGQPGCTDFERKNFQKILTAGNLVDSYRYLQNIKLNEDKKNNIKHTPNINDNIYTWRCPFLLGKSCNKAMRIDHFIVSKEFLNRINKIHIQGFSVFHNNFYGSDHCPVILYLKNE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have a high affinity for Mg(2+).2. The protein must exhibit the following characteristics: Has 3'- phosphatase activity; cleaves 3'-phosphate from blunt, recessed and gapped dsDNA templates, followed by 3'-5' exonuclease activity; Multifunctional protein that plays a central role in mitochondrial DNA base excision repair (BER) pathway induced by oxidative stress; Has 3'-5' exonuclease; can use dsDNA templates with 3'-OH termini including blunt-end, gapped and mismatched 3'-recessed; Plays a role in merosome infection of host erythrocytes .; Has nucleotide incision repair (NIR) activity; acts on dsDNA with oxidized bases thymine glycol and 5,6-dihydro-2'-deoxyuridine; Has apurinic/apyrimidinic (AP) endonuclease activity towards double-stranded DNA (dsDNA); Has RNase H-like activity; cleaves RNA on 3'- recessed RNA-DNA duplex.3. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for Exonucleolytic cleavage in the 3'- to 5'-direction to yield   nucleoside 5'-phosphates..4. The presence of Mg(2+), Mn(2+) is crucial for the protein to function optimally under specific conditions.5. Prioritize Basic and acidic residues, Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "MGLAELRELIEPEETDLRALAGREIAIDAFNALYQFLTTIMKDGRPLMDSRGRITSHLNGLLYRTVNLVEEGIKPVYVFDGEPPDLKRETLERRRERKEEEALNHQKQKRKVAIDASMCLYQFLIAIRQDGSQMQSEDGETTSHLMGMFYRTIRMIDNGIKPVYVFDGKPPDMKSGELEKRTERRAEAEKALTEAKEKGDAKEAEKFERRLVKVTKQQNEEVKQLLGLMGIPVVEAPCEAEAQCANLVKAGKVYGTATEDMDALTFGSCVLLRHLLAPEAKKIPIKEFHLARVLEEMQLTKDQFIDLCILLGCDYCGTIRGIGPKKAVELIKQHKNIETVLENIDQTKYPPPADWPYKRARELFHEPEVMKCDEVELTWKDPDVRTKKAILTLIDVNSLNPIVYISRFFGVVNRYISYIVPILWHPINSNILVSIDLSQDVQKILNFFKRNSILNVNYKEIFLMGIRFIYVNRCPILIPTNVIRMKDRIRLRINYKLFQNNLVLLRKNIFLKKKLKKFLCSIAEAPKYNGSNVDLKMYNSFFSYIDNNVIKNIHSTLPKRIKINFMKYDNRINQLFILFLARYRPDMLDYSEKYFWIQRYLNIFSYANIQKYENKILKLIVKYKNHVRNVQLLEELFEYVERTKKAILTLIDVNSLNPIVYISRFFGVVNRYISYIVPILWHPINSNILVSIDLSQDVQKILNFFKRNSILNVNYKEIFLMGIRFIYVNRCPILIPTNVIRMKDRIRLRINYKLFQNNLVLLRKNIFLKKKLKKFLCSIAEAPKYNGSNVDLKMYNSFFSYIDNNVIKNIHSTLPKRIKINFMKYDNRINQLFILFLARYRPDMLDYSEKYFWIQRYLNIFSYANIQKYENKILKLIVKYKNHVRNVQLLEELFEYV",
    "response#2": "MGNADLRSLAALSDVSFDDLGGSVVAVDAHNWLYRYLTTTVKFTSESKYTTSNGEEVANLIGVVQGLPKFFEHDMTPVFVFDGAVTDLKDDEVEKRREQRQKAVGIPVVEAPCEAEAQCANLVKAGKVYGTATEDMDALTFGSCVLLRHLLAPEAKKIPIKEFHLARVLEEMQLTKDQFIDLCILLGCGIPVVEAPCEAEAQCANLVKAGKVYGTATEDMDALTFGSCVLLRHLLAPEAKKIPIKEFHLARVLEEMQLTKDQFIDLCILLGCFRTKKAILTLIDVNSLNPIVYISRFFGVVNRYISYIVPILWHPINSNILVSIDLSQDVQKILNFFKRNSILNVNYKEIFLMGIRFIYVNRCPILIPTNVIRMKDRIRLRINYKLFQNNLVLLRKNIFLKKKLKKFLCSIAEAPKYNGSNVDLKMYNSFFSYIDNNVIKNIHSTLPKRIKINFMKYDNRINQLFILFLARYRPDMLDYSEKYFWIQRYLNIFSYANIQKYENKILKLIVKYKNHVRNVQLLEELFEYVADHLSLLEGQLAHVEVRFQPENDQNLKVVWFKDDLPLEQKARLMFNIEAGRASFDIDYVQLSDGGLYKCLVVNQKGKAEATFTISVG",
    "response#3": "MGLAELRELIEPEETDLRALAGREIAIDAFNALYQFLTTIMKDGRPLMDSRGRITSHLNGLLYRTVNLVEEGIKPVYVFDGEPPDLKRETLERRRERKEEARLDAERRQHESRTKKAILTLIDVNSLNPIVYISRFFGVVNRYISYIVPILWHPINSNILVSIDLSQDVQKILNFFKRNSILNVNYKEIFLMGIRFIYVNRCPILIPTNVIRMKDRIRLRINYKLFQNNLVLLRKNIFLKKKLKKFLCSIAEAPKYNGSNVDLKMYNSFFSYIDNNVIKNIHSTLPKRIKINFMKYDNRINQLFILFLARYRPDMLDYSEKYFWIQRYLNIFSYANIQKYENKILKLIVKYKNHVRNVQLLEELFEYVGVPIVQAPCEAEAQCAELCKGGKAWATGSEDMDSLTLGTTILLRRLTFSEARKLPIMEIELEKVLDGLDLTHDQFVDLCILLGCFPRTKKAILTLIDVNSLNPIVYISRFFGVVNRYISYIVPILWHPINSNILVSIDLSQDVQKILNFFKRNSILNVNYKEIFLMGIRFIYVNRCPILIPTNVIRMKDRIRLRINYKLFQNNLVLLRKNIFLKKKLKKFLCSIAEAPKYNGSNVDLKMYNSFFSYIDNNVIKNIHSTLPKRIKINFMKYDNRINQLFILFLARYRPDMLDYSEKYFWIQRYLNIFSYANIQKYENKILKLIVKYKNHVRNVQLLEELFEYVPKLFNFFFKYRTKKAILTLIDVNSLNPIVYISRFFGVVNRYISYIVPILWHPINSNILVSIDLSQDVQKILNFFKRNSILNVNYKEIFLMGIRFIYVNRCPILIPTNVIRMKDRIRLRINYKLFQNNLVLLRKNIFLKKKLKKFLCSI"
  },
  {
    "reference": "MKDLFKIYSNYQPAGDQPTAIASLIDGLESGLAKQTLLGVTGSGKTFTIAHVIQAMKRPTLIMAPNKTLAAQLYGEFKAFFPDNAVEYFVSYYDYYQPEAYVPASDTFIEKDASINEHIEQMRLSATKALIERKDAIIVATVSAIYGLGDPDSYLRMLLHLSRGEQSDQRKILKRLAEMQYTRTNLSLERGQFRVHGDVIDIFPADSEKEAIRIELFDDEVDNIARFDPLTGEILQRLPRVTIFPKTHYVTPRERILETVEKVKAELQERLAELNAQNKLVEAQRLEQRTCFDIEMMLELGYCSGIENYSRYLSNREAGEAPPTLFDYLPPDALLIIDESHVTVPQIGGMYRGDRARKETLVNYGFRLPSALDNRPLRFEEFEERSPQTIYISATPGPYEQEHSDNVAEQVVRPTGLIDPEVEIRPVKTQVDDLMSEIRQVIAQGSRILVTTLTKRMAEDLTEYLSEHGIKVRYLHSDVDTVERMEIIRDLRLGEFDVLVGINLLREGLDMPEVALVAILDADKEGFLRSERSLIQTIGRAARNVKGRAILYADTMTGSMQRALMETERRREKQKAFNLKHGITPKGINKSVEDILEGAYIGKRKTMVAEQAPRYTHWSPQELAKQINALEKQMYAHAQNMEFELAAKIRDEYLLLKEQLMKI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Then UvrB probes one DNA strand for the presence of a lesion; A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities.2. The protein contains novel Helicase ATP-binding, UVR domains that confer a unique function or activity.3. The designed protein should be involved in SOS response by localizing to cytoplasm and exhibiting excinuclease ABC activity, helicase activity, ATP binding, ATP hydrolysis activity. The designed protein sequence is ",
    "response#1": "SPYKPLGDQPKAINSLVDGINKGEKEQTLLGVTGSGKTFTMANVIEKVQKPTLVISHNKTLAAQLYEEFKEFFPDNAVEYFVSYYDYYQPEAYVPRTDTFIDKESSVNEEIDIMRHSATQSLLSRDDVIVVSSVSCIYGIGSPEDYGEFAFGIAVGDNYDRSDIIRKLVFMQYERNDIEFARGHFRVRGDVIEINPVHGTPPVRVELFGDEIDAISLIDKVTGKKTESLKRYMIFPAKHFVVGQDKMDTAIRNISDELDERLNEFNLSNKLLEAQRLEQRTRFDIEMLQEMGYCPGVENYSMHLSGRKWGEKPYSLLKYFPEDYLTIIDESHVTLPQIRGMYNGDRARKETLVEHGFRLPSAKENRPLRFDEFESSINQIIYVSATPGAYELSRSSNIVEQIIRPTGLVDPEVIIDSLQNQHRFQTLLGATGTGKTFTIAAVIEKIGRPTLVLAHNKTLAAQLCNELRQFFPNNAVEYFISYYDYYQPEAYIPVSDTYIEKSSSINDEIDMLRHSATRSLFERRDVIVVASISCIYGLGMPAEYLKAAISLTVGQEFDQRQLLRALVSVQLHLQIGDKYERKNLINKLIELKYQRNEINFQRGTFRVRGDIIEIIASSSKEIGIRIIFFGNEIENIQNFYVLNGKAIANLKLITLFPASLE",
    "response#2": "KPTGDQPRAIQELIEGLEQNKKSQVLLGVTGSGKTFTIANVIKAANRPVLLLSHNKTLASQLYSELKDFFPNNRVEYFVSYFDYYRPEAYMPNTDTYIDKTTKSNWDLEEMRMSTLNSISTKRDTIVVASVASIYGAVQ",
    "response#3": "SPYKPLGDQPKAINSLVDGINKGEKEQTLLGVTGSGKTFTMANVIEKVQKPTLVISHNKTLAAQLYEEFKEFFPDNAVEYFVSYYDYYQPEAYVPRTDTFIDKESSVNEEIDIMRHSATQSLLSRDDVIVVSSVSCIYGIGSPEDYGEFAFGIAVGDNYDRSDIIRKLVFMQYERNDIEFARGHFRVRGDVIEINPVHGTPPVRVELFGDEIDAISLIDKVTGKKTESLKRYMIFPAKHFVVGQDKMDTAIRNISDELDERLNEFNLSNKLLEAQRLEQRTRFDIEMLQEMGYCPGVENYSMHLSGRKWGEKPYSLLKYFPEDYLTIIDESHVTLPQIRGMYNGDRARKETLVEHGFRLPSAKENRPLRFDEFESSINQIIYVSATPGAYELSRSSNIVEQIIRPTGLVDPEVIIDSLQNQHRFQTLLGATGTGKTFTIAAVIEKIGRPTLVLAHNKTLAAQLCNELRQFFPNNAVEYFISYYDYYQPEAYIPVSDTYIEKSSSINDEIDMLRHSATRSLFERRDVIVVASISCIYGLGMPAEYLKAAISLTVGQEFDQRQLLRALVSVQLHLQIGDKYERKNLINKLIELKYQRNEINFQRGTFRVRGDIIEIIASSSKEIGIRIIFFGNEIENIQNFYVLNGKAIANLKLITLFPASLSRVQIDDLVGEINKRVDAHERVFITTLTKKMAEDLTDYLKDMGIKVRYLHSDIKTLERTQIIRDLRLGKFDVLIGINLLREGIDVPEVSLIAILDADKEGFLRAERSLIQTIGRASRNEHGKVIMYADKVTDSMKAAIDETQRRRTIQEKFNEEHHITPKTIIKP"
  },
  {
    "reference": "MAILRLENGTTYTHLDDIAPELAPLNIRLKHWSVGENPEIHQLLAQDTLEDLEKGYVLQSLDHYFLELQRFSGYQSRDLIVLNPDIPNLDALLTKFERPHTHADDEVRYVIDGEGVFGFVRADDSQVELTVQAGEYINVPAGTEHWFYLTPARRLKAVRYFAGTDGWVPRYTNAIIRFRQAAAFSGSR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway..2. A protein that can modulate the overall flux of metabolites through the L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6, L-methionine biosynthesis via salvage pathway pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "GMASNESYDGTPATGITSVVTSSQYLEGPSQEGGRGGSDEEGIHGPTGSNSSGGVEGVIISGHEDHLIRFFR",
    "response#2": "MRFLQLEWHNHERARNAWDIERAEMKAKIAKQEGEVRSAKKLNDQLNRHIRMLEQALTNERKNKANAAGGDAADDKGKKTGAGLTKPQNTKHNSFLDVDSEFLDRSEADRDGLREKSKLYLTKCVEEITYLLTPS",
    "response#3": "GMASNESYDGTPATGITSVVTSSQYLEGPSQEGGRGGSDEEGIHGPTGSNSSGGVEGVIISGHEDHLIRFFR"
  },
  {
    "reference": "MKLQLAAVATLAVLTSPAFGRVLPDGKYVKIPFTKKKNGDNGELSKRSNGHEKFVLANEQSFYSVELAIGTPSQNLTVLLDTGSADLWVPGKGNPYCGSVMDCDQYGVFDKTKSSTFKANKSSPFYAAYGDGTYAEGAFGQDKLKYNELDLSGLSFAVANESNSTFGVLGIGLSTLEVTYSGKVAIMDKRSYEYDNFPLFLKHSGAIDATAYSLFLNDESQSSGSILFGAVDHSKYEGQLYTIPLVNLYKSQGYQHPVAFDVTLQGLGLQTDKRNITLTTTKLPALLDSGTTLTYLPSQAVALLAKSLNASYSKTLGYYEYTCPSSDNKTSVAFDFGGFRINAPLSDFTMQTSVGTCVLAIIPQAGNATAILGDSFLRNAYVVYDLDNYEISLAQAKYGTGKENVEVIKSTVPSAIRAPSYNNTWSNYASATSGGNIFTTVRTFNGTSTATTTRSTTTKKTNSTTTAKSTHKSKRALQRAATNSASSIRSTLGLLLVPSLLILSVFFS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Include a signal peptide as an essential component of the protein.2. A protein that contributes to fungal-type cell wall organization, proteolysis by functioning in fungal-type cell wall, extracellular region with aspartic-type endopeptidase activity.3. The protein must exhibit the following characteristics: Cleaves proteins C-terminally to mono- and paired-basic residues. The designed protein sequence is ",
    "response#1": "GLAWIPGTDLGYSDNFVNKGINWYYNWGSYSSGLSSSFEYVLNQHDANSLSSASSVFTGGATVIGFNEPDLSAAGNPIDAATAASYYLQYLTPLRESGAIGYLGSPAISNVGEDWLSEFMSACSDCKIDFIACHWYGIDFSNLQDYINSLANYGLPIWLTEFACTNWDDSNLPSLDEVKTLMTSALGFLDGHGSVERYSWFAPATELGAGVGNNNALISSSGGLSEVGEIL",
    "response#2": "SHWSTFYKEEDFAKIASQGFNLVRIPIGYWAFTTLSHDPYVTAEQEYFLDRAIDWARKYGLKVWIDLHGAAGSQNGFDNSGLRDSYKFLEDENLSATMKALTYILSKYSTDVYLDTVIGIELLNEPLGPVIDMERLKNLLLKPAYDYLRNKINSNQIIVIHDAFQPYHYWDGFLNDEKNEYGVIIDHHHYQVFSN",
    "response#3": "GLAWIPGTDLGYSDNFVNKGINWYYNWGSYSSGLSSSFEYVLNQHDANSLSSASSVFTGGATVIGFNEPDLSAAGNPIDAATAASYYLQYLTPLRESGAIGYLGSPAISNVGEDWLSEFMSACSDCKIDFIACHWYGIDFSNLQDYINSLANYGLPIWLTEFACTNWDDSNLPSLDEVKTLMTSALGFLDGHGSVERYSWFAPATELGAGVGNNNALISSSGGLSEVGEIQ"
  },
  {
    "reference": "MVRLPLPQRFISSKSATPIQQSRNQSPLRSPMDSTLTLKVNVIKGRDLAAKDRGGTSDPYLVVSLGSARESTPTISKTLNPDWNVTFDLPISGVPLLECVCWDRDRFGRDYMGEFDIPLEEIFAEGETQHQAKWYTLESKRRRRKKKESIVSGQILIQFSLLDTANLGANPADTYRKFRSIVCAVEEEEDDTALESVDQDENDKDEESSDENDDPTKSEVVEKRRRRLRIARLKRKSLAARAYQFSGASNGVQGVIFLEINRISNLPPERNMTRTSFDMDPFVVTSLGRKTLRTRVIRHNLNPVYDEKMVFQVMKHEQSYTMSFTVVDRDKLSGNDLVATAELPLQTLIAAAPEADPETGLYKLQEPSKPGQPLQSQRSRFRLPMSRSNSASSLGKSSKPTLSSQSSSPSLITTQTRSDQSLPTPPTSVPSEMSNPLLDVPSISNLAPKIDETPPPPQSSPDSEDLKTYTIPLLLKNKERWEDKYSPELYIKAKYMPYKALRQQFWRVMLKQYDADDSGRLSKIELTSMLDTLGSTLKESTIDSFFSRFSAENESGEFNDLTYDQAVICLEDALQKVQKSSFGRVDSKLRKLIPGSLSSSGDRSPDTSSGADETTDLEGADGQPQQLDLSEFPEGEQPSPGYGELVPDDLADERGEEHVIEIGECPLCHQPRLSKRSDADIITHLATCVSQDWRQVDNLVMGGFVTSSQAQRKWYSKVVTKIGYGGYRLGANSANILVQDRITGQINEEKMSIYVRLGIRLLYKGIKS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein's functionality will benefit from Acidic residues, Polar residues compositional bias in its structure.2. The protein must be engineered to have a high affinity for pyruvate in order to increase its catalytic activity.3. A protein that can perform calcium ion binding in Golgi membrane, Golgi stack, endosome membrane to support phosphatidylethanolamine biosynthetic process.4. The Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity, Charge relay system; for autoendoproteolytic cleavage activity active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.5. The designed protein must have at least one EF-hand domain.6. For general function, the protein need meet that Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer); Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.7. The protein design should take into account the physiological conditions of the phosphatidylethanolamine from CDP-diacylglycerol: step 2/2 pathway. The designed protein sequence is ",
    "response#1": "MKSLTLRVNVVEARNLMAKDRRGTSDPFVVIKLGGQSNKTSVIPRNLSPTWNEMFEFVLTPVLFDASLDFIVWDKDLIGKDFLGQLSIPISDILRISDAASFDDLFKEYEQSNNQNSSGRNHFSPIAGEIPLVILRVQIIGCKDLLAKDRNGYSDPFVCASVLSTRHQTPVSKKTTSPDFPPRESTFDFPLYLSLADKLGVLELVVWDKDMFTKKDYLGEVALSLEEWFGMNEDGMGEKCKWYPLEDGLSEGRKSLAARAYQFSGAGNGVQGIVFMEIVRVTDLPPERNVTRTSFDMDPFVVTSLGRKTLRTPVVRHNLNPTYNEKMVFQVMKHEQLYTISFTVMDRDKFSGNDFVASAGFPLQTLIHAAPEADPEPGYHKIKLYRKSLAARAYQFSGAGNGVQGIVFMEIVRVTDLPPERNVTRTSFDMDPFVVTSLGRKTLRTPVVRHNLNPTYNEKMVFQVMKHEQLYTISFTVMDRDKFSGNDFVASAGFPLQTLIHAAPEADPEIVMLEIQSAADLPRLKNMTRTGWDMDPFVVISFGKKVFRTRVIRHSLNPTWDEKLLFHVRRYETAFKIQLTVLDWDKLSSNDHVGDASFNVSELLANGLDFIVWDKLRLKRKSIAARAYQFSGASNGVQGIIFLEINRIMTRTSFDMDPFVVTSLGRKTLRTRVIRHNLNPVFDEKMVFQVMKHEQLYTMSFTVVDRDKLSGNDLVATAELPLQTLISTAPEAD",
    "response#2": "MKSLTLRVNVVEARNLMAKDRRGTSDPFVVIKLGGQSNKTSVIPRNLSPTWNEMFEFVLTPVLFDASLDFIVWDKDLIGKDFLGQLSIPISDILRISDAASFDLRVQVVGCNDLIAKYRNGHSDPFVVISLLRDRHQTPVMRRTLNPTFTPKDATFDFPIYLSLADKLGVLEFVVWDKDMLKKDYLGEANIPLELPMISEGRNSGKVSFSPIPGETPIALLRVQILCCEDLLPKDRGGTSDPFVVVSLLGNKQQTSVVKKTLNPTWSAKDATLDLPIYLSLADKLGALELLVWDKDMLRKDYLGEHALPLEDWFKGTAYSFDIVMLEIQSADDLPRLANMTRTGWDMDPFVVISFGKKVFRTRVIRHSRNPVWDEKLLFHVRRYETSFKVQLAVLDWDKLSSNDHIGDVSFDVKQLIDSAILRVQVLSCTNLLAKDRNGHSDPFVVLSVLANRHQTPVSKRTVNPTFNPKDATFDFPIYLSLADKLGVLELVVWDKDVLKKDYLGEAAIPLDLSIVPEPIVMLEIQSADDLPRLANMTRTGWDMDPFVVISFGKKVFRTRVIRHSRNPVWDEKLLFHVRRYETSFKVQLAVLDWDKLSSNDHIGDVSFDVKQLIDSAPQPDPSNSVTIDTSEDDNAPEIKLRVVVSSARGLTCKDKDDAVDAFAIVACGGTIQRTQVCKKSNCPEWNTTFEIPLGKIPPRRFASGLLVTVYDKDRFKSSFIGRVHFNLIDVFDETMAAPIAIVMLEIQSAADLPRLKNMTRTGWDMDPFVVISFGKKVFRTRVIRHSLNPTWDEKLLFHVRRYETAFKIQLTVLDWDKLSSNDHVGDASFNVSELLANAPQKD",
    "response#3": "MKSLTLRVNVVEARNLMAKDRRGTSDPFVVIKLGGQSNKTSVIPRNLSPTWNEMFEFVLTPVLFDASLDFIVWDKDLIGKDFLGQLSIPISDILRISDAASFDDSSSDSVSGDYLDKADTKAFDHNAQMGLLTIDIVGATNLPYETNVTRTSFDCDPFVVISYGRKTFRTRTVRHTLQPTWNERVFLHVRHSEHQLGWAVNLSVYDYDKFSNNDYIGTTELKISDVVATCKRLPTTLKTTVLKGRSLAAKDRGGTSDPYLVVTLGDARESTPTISKTLNPDWNVTFNLPVVGVPLLECICWDRDRIGRDYMGEFDIPLEEIFADGEVHQQPRWYLKVVVVKARNLAAKDKNGTSDPYLVVTLGDSKNATQSVSKNLNPEWNVTLQMPVNSANALLLDCVCWDKDRFGKDYLGEFDLALEDIFTADEVDIGPQWFPLKVVVLRGRCLAAKDRNGTSDPYLVVTLGDARQSTPTIPKTLNPEWNVTFEMPIVGVPLLECICWDHDRFGKDYMGEFDIPLEEIFAEGQIHQQPKWYTPTGLPSRSESPMRPKNDGTGPAGVAANGLALRVVIMRARNLAAKDRSGTSDPYLVVTLGDARVVTHSVPKTLNPEWNVIEELPISSVQSLVVGVICWDKDRFGKDYLGEFDLALEEIFQNEVAEQEPRWRLKRKSIAARAYQFSGASNGVQGIIFLEINRIMTRTSFDMDPFVVTSLGRKTLRTRVIRHNLNPVFDEKMVFQVMKHEQLYTMSFTVVDRDKLSGNDLVATAELPLQTLISTAPEAD"
  },
  {
    "reference": "MRNYRGSKVVIIGLGITGLSCVNFFLDRGVIPKVIDTRIYPPGIKKIPHVVQCYLGAFNDIWLLSATLIVVSPGVRLDHPVLIEALKLGIEIIGDIELFTREATAPIIAITGSNGKSTVTQLVSRMARIAGWHVGVAGNIGVPVLSLLNKSYQLYILEISSFQLDTTYSLRAIAAAILNVSEDHMDHYPGGLKQYWFSKQRIYKNAKICVMNALDFLTIPIYHEYDYCISFGENEDSADYYLKYYKGHTWIVAYNEYVLNCSEMRINNRINYINALSALALSDIIKIPRSVSLKVLCQFSGLAHRCQLIYKNHGVSWINDSKATNVSATKEAINNLKLCGTLHLILGGDGKLADFSSLKHLIKQHEIHLYCFGKDGLLLTTLGFSDVILTNTMIQAMRIINRRIKAKDIVLLSPACSSLDQFKSFEMRGLIFTCFAREFR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be optimized for stability and expression in cytoplasm where it will participate in cell division, cell cycle. The designed protein sequence is ",
    "response#1": "PTASTFVQHFTRYYGDGKEEELLKEAAHRFTDGSLLTYGFHRYLPSVVAASAIFLARLHVLGHEPWSRDLAELTGYEAIDLMGCVCDMYSQIACPRFAPFQVCEHVPDSSHVSADHEEVC",
    "response#2": "TLRAMETNPAERPSPYFLETTQGGRMTALVRASMIAFMDEFSRFYELADGTLQRAAYFLDRYLSVTPESDDVLQLRLVGATAVFLAAKYEDQYTLRKIDASMVAARCGYTSETRHKMVSCMETEILAALDYNLL",
    "response#3": "TLRAMETNPAERPSPYFLETTQGGRMTALVRASMIAFMDEFSRFYELADGTLQRAAYFLDRYLSVTPESDDVLQLRLVGATAVFLAAKYEDQYTLRKIDASMVAARCGYTSETRHKMVSCMETEILAALDYNLI"
  },
  {
    "reference": "MATMNVKQKTIKSEVSVQGVGLHTGANVTLTFCPAPDNHGFKFQRIDLPGSPIVDADCDNVTDTARGTTISQNGASVSTVEHVMASLVGMDLDNVLIKLDGPETPIMDGSAILFLEALESAGVQLQDLDREYFQIPHNITYTEPDRKVEIVAMPLDDYRFTCMIDYNSPVLGSQHAGISSIAEFKKEIASCRTFCFLHELEYQLQNNLIKGGDLNNAIVIVDKEVTKEELDHLAKIFNRTAMEVAPQGILNNMELRYQNEPARHKLLDMIGDLALVGVHLKGHIMAARPGHAANVAFAKKIKAAIKKEKNKKTQHVYDPSVKPLYDVVQIMEILPHRQPFLFIDKILELSKTHVVGVKNVTMNEEFFKGHFPGAPVFPGVIQIEAMAQTGGILVLSTVPDPRNYLTLFLKIDNVRFRAQVLPGDTIVFRCDLMEPIRRGIAQMKGVGMVGGKVVVEAEMMAQIVKVKESESVS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.2. A protein with 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity, 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity, 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity, UDP-3-O-acyl-N-acetylglucosamine deacetylase activity functions is required to enable fatty acid biosynthetic process.3. The protein's active site, Proton donor must be carefully engineered to ensure that the amino acids involved in a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine +   H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate are properly positioned for optimal catalysis.4. For general function, the protein need meet that Involved in unsaturated fatty acids biosynthesis; Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs. The designed protein sequence is ",
    "response#1": "MSNRYKGIDLIXALXPRXXGXVXPXDXIVXPXEAXAXXTXAXVXAAXGXATTXLPXGXPWXIVTDPSRELGXFAAXFYGEPSGXMXLIAXIGXDXXGXXPXKXAXGVXPXVXPIXXPXDPXQXXAXPXRXPXXVXHQXPXPXXXDXTTQXXIMQ",
    "response#2": "MDFDDLGVALRRGMDASLGTVDALGRAWGPRLAPVADGRLLEFASNDYFGIARHPAIVAAARAAVERFGVSAGASRLVSGSTPLHAAVEAAAAELHGGPALLYPSGFQANLAAVTALAADAGTVVISDALNHASIVDGCRLAGAQLRVYAHNDVAGLEAQLRAWSAQARAVVVESVYSMDGDRAPIADLVALADRYGALVVVDEAHSVGVLGPNGGGVAGRAGLTGQVDLLTATFGKALGSAGAVVAGSAALVDYLVNRARGLVYSTALPPAVAAAARAAIAIARADGWRDRLRARAADLVHGLRAAGFDVGDSASQIVPIRVGDPARTAALSERLRAEGVLVKAIRPPTVPEGTARLRVSVTADHTEEDIDQLVAALRD",
    "response#3": "MSVLRGAGGDAGRALPVWPPPGSAGPDLAALAAALDTATGTGADGPDVASVAVAGGREARDDVVLARAPGAVADAAVAEARARGAAVVLVDAAGAAGFAGVDAAGVVVLGVGDPRGVLGLVAGRLHGDPSARLAVVGTYGSAGKTTVTSLLARALTDAGLRVGVIGTDGVRIGDGERVPGLWATPGRTGVARRLAAAGVDVAAVESDSLGPAALERFGLDVAVLTNVTCDHLDYHGTLDAYAAAKRRFLTQLAPDGLAVVNADDPHSAAMADVCAAPVVRVGVDPAADVRGEVVESALSGTGVRARLLAGGRELAVALPVLGRHNVYNALAAATAGLALGVPVPAVAAGVAACPGVP"
  },
  {
    "reference": "MEPVDPNLEPWNHPGSQPTSPCNNCYCKKCCWHCQLCFLKKGLGISHGRKKRKHRRGAPPSSKDHQDLIPKQPLSIIRGNPTGPKESKKEVESKAETDPFA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Tat can reactivate a latently infected cell by penetrating in it and transactivating its LTR promoter; Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication; The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive; Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation; This also contributes to the increase in proviral transcription rate, especially when the provirus integrates in transcriptionally silent region of the host genome; In the cytoplasm, Tat is thought to act as a translational activator of HIV-1 mRNAs.; Other factors such as HTATSF1/Tat-SF1, SUPT5H/SPT5, and HTATIP2 are also important for Tat's function.2. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein should have metal ion binding, protein domain specific binding within host cell nucleolus to regulate negative regulation of peptidyl-threonine phosphorylation, modulation by virus of host chromatin organization, positive regulation of transcription elongation by RNA polymerase II.4. The protein design should emphasize Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "MEPVDPNLEPWNHPGSQPSTPCNKCYCKQCCWHCQLCFLKKGLGISYGRKKRKHRRGTPQSSKDHQNPVPKQPLPTTRGNPTGPKESKKEVASKTETDPCA",
    "response#2": "MEPVDPNLEPWNHPGSQPTTPCSKCYCKKCCWHCQLCFLKKGLGISYGRKKRKHRRGTPQDSKDHQNPVPKQPLPTTRGNPTGPKESKKEVESKTETDPCD",
    "response#3": "MEPVDPNLEPWNHPGSQPTTPCSSCYCKQCCFHCQKCFLTKGLGISYGRKKRGQRRGTPQSSKDHQNPIPKQPSSQPRGDPTGPEESKKKVESKTEADPCD"
  },
  {
    "reference": "MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAKAFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGMANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGDIGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGFAIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAAVADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNVEEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLLLDEIVTRYDEKNRR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that can modulate the overall flux of metabolites through the coenzyme A biosynthesis pathway, enhancing or suppressing its activity as needed.2. The protein must exhibit the following characteristics: Catalyzes two sequential steps in the biosynthesis of coenzyme A; In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine..3. The ideal protein would be one that plays a critical role in pantothenate catabolic process, coenzyme A biosynthetic process through phosphopantothenoylcysteine decarboxylase activity, phosphopantothenate--cysteine ligase activity, metal ion binding, identical protein binding, FMN binding within phosphopantothenoylcysteine decarboxylase complex.4. The designed protein must have a Proton donor active site that is highly conserved among related enzymes.5. turn-strand-strand-helix-strand-strand-helix-helix-strand-strand-strand-helix-strand-strand-helix is the desired arrangement for the engineered protein. The designed protein sequence is ",
    "response#1": "MRIIVGVAGGIAAYKACEVVSRLVKAGHSVRVVMTPAAAQFVAPLTFRALSGHAVGIEAADEPAGPLSHVQLAHWAEALVIAPATASLMSRLAHGQTDDLLGLVYLGIRGPVVAAPAMEPEMWSHPRTQANASVLRADGVRLVGPAVGRMASGQEGLGRMAEPAEIVAALEDALLAQPPLATPLSGKRVLISAGGTREHLDPVRFIGNPSTGKMGIALAQAAMHRGAQVTLVHAPMDGRLVESVGEIGRAIAITSAAEMHQAMLEHFSQADITIMSAAVADVKPANYSQEKLPKNSLPTALPLEPVPDILAELGRLKQPHQRLIGFAAQTGDIVTPALEKMRRKKVDAIVANPIDKSDSGFGSDNNQAIALWSPATELSGKRVLISAGGTREHLDPVRFIGNPSTGKMGIALAQAAMHRGAQVTLVHAPMDGRLVESVGEIGRAIAITSAAEMHQAMLEHFSQADITIMSAAVADVKPANYSQEKLPKNSLPTALPLEPVPDILAELGRLKQPHQRLIGFAAQTGDIVTPALEKMRRKKVDAIVANPIDKSDSGFGSDNNQAILWYEPAASKATLMRDEQIHLIQKKPYLFMAAAVSDYVPKFVQNGKLKKEMLGNSFELSLTKNVDILNSIDKDGLVTIGFKAEMDIESAMKSASKMIDTKGIDAVCLNILKDSSSFGSDRNMVELYWREGGELKQHAAAGQHALVTAGPTIEELDPVRYLSNRSSGKIGYALAETLRDKGAQVTLVSGPTSFSDPEGVEVVRVESAEDMFEAVKQRYDNVDIVFKAAAVSDYTPTEMLPHKLKKQEGELSVTFKRTPDILQYLGEHKSHQYLVGFAAETRDIERYAQDKLERKNADVIIANNVGDRSIGFSSDNNDYT",
    "response#2": "MRIIVGVAGGIAAYKACEVVSRLVKAGHSVRVVMTPAAAQFVAPLTFRALSGHAVGIEAADEPAGPLSHVQLAHWAEALVIAPATASLMSRLAHGQTDDLLGLVYLGIRGPVVAAPAMEPEMWSHPRTQANASVLRADGVRLVGPAVGRMASGQEGLGRMAEPAEIVAALEDAFAAAKDPHYLTGQRVMVSAGPTQEPIDPVRFLSNRSSGKMGYALAQAARERGADVLLVSGPTALPVPPGVRCLHVETAEDMAKALTTNFRWSTIVIMAAAVADFRPDKPSGQKIKKQTGAWKTLALAPTEDILHTLSRQRTRQILVGFAAETEALLPHARKKLQDKDLDLIVANDVTADGAGFGSDHNAATLLTRDGDEQAVPLGSKSALAHVVWDLVAPLLD",
    "response#3": "MRIIVGVAGGIAAYKACEVVSRLVKAGHSVRVVMTPAAAQFVAPLTFRALSGHAVGIEAADEPAGPLSHVQLAHWAEALVIAPATASLMSRLAHGQTDDLLGLVYLGIRGPVVAAPAMEPEMWSHPRTQANASVLRADGVRLVGPAVGRMASGQEGLGRMAEPAEIVAALEDAVRAFQPRRLVAGQHALVTAGPTIEELDPVRYLSNRSSGKIGYALAETLRDKGAQVTLVSGPTSFSDPEGVEVVRVESAEDMFEAVKQRYDNVDIVFKAAAVSDYTPTEMLPHKLKKQEGELSVTFKRTPDILQYLGEHKSHQYLVGFAAETRDIERYAQDKLERKNADVIIANNVGDRSIGFSSDNNDYTSVYADNGSVAYAGQHALVTAGPTIEELDPVRYLSNRSSGKIGYALAETLRDKGAQVTLVSGPTSFSDPEGVEVVRVESAEDMFEAVKQRYDNVDIVFKAAAVSDYTPTEMLPHKLKKQEGELSVTFKRTPDILQYLGEHKSHQYLVGFAAETRDIERYAQDKLERKNADVIIANNVGDRSIGFSSDNNDYTDAVFLDARGRETADTGLDKQALAHLLLDRLAALRP"
  },
  {
    "reference": "MQNHHHQQSSYGGGYPGQAYREQHPPPNPYGYGQPSPQPGYGAPPPHNGYATAVGIRPAAATYWKRGVQWKTAWYVIAFGAGRNASALESVKYAAADTCNTGMNQYQNPYSHGHQGGPPPPPTDPVAFGHGAPQGYSFQYSRCTGKRKALLIGINYFGQKGQLRGCINDVKNMSTYLNQNFGYAREDMVLLTDDQQNPMSQPTKANILRAMHWLVKDAQPNDSLFFHYSGHGGQTPDLDGDEEDGYDEVIYPVDFRQAGHIVDDEMHRIMVRPLRPGVRLTAIFDSCHSGSALDLPYIYSTQGILKEPNLAKEAGQGLLGVVSAYARGDMGGMVSTAVGFLKRATKGDEAYTRSKQTKTSPADVIMWSGSKDSQTSQDAQIAGQATGAMSWAFITALRKNPQQSYVQLLNSIRDELATKYSQKPQLSCSHPLGKH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must have a active site that is structurally stable and resistant to denaturation.2. The designed protein's functionality will benefit from Pro residues compositional bias in its structure.3. For general function, the protein need meet that Involved in cell death (apoptosis).4. The protein should be able to modulate proteolysis in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "RRKALLIGINYIGQPNQLRGCINDVANVSRYLNERCRYRREDMVILTDDQENPLSIPTKNNILRAMQWLVKDAQPNDSLFIHFSGHGGRTPDLDGDEEDGYDDVIYPVDYRTAGHIVDDEMHAIMVRPLRPGVRLTAIFDSCHSGTALDLPYVYSTQGVLKEPNLAKEAAMDLFSAISSYGQGDLTGMAKTAIGFFKKAAIGDSARQRTVMTKTSPADVVMFSGSKDTQTSADTFQDGEARGALSWAFVKTLQERPHQSYLQLLNSIRAELEGKYTQKPQLSCSHR",
    "response#2": "KRKALLIGINYFGQRGQLRGCINDVKNMSSYLHENFGYQRDDMVLLTDDQQNPMSQPTKQNILRAMHWLVKDARPNDSLFFHYSGHGGQTKDLDGDEEDGYDEVIYPVDFRQVGHIVDDEMHRIMVQPLQPGVRLTAIFDSCHSGTALDLPYVYSTQGVLKEPNLAKEAGQGLLGVISSYSQGDMSGVASNLMGFFKKATTGDDAYNKTLATKTSPADVIMWSGSKDDQTSADATIAAQATGAMSWAFITAMKKNPQQSYVQLLNSIRDELATKYTQKPQLSCSHPW",
    "response#3": "KRKALLIGINYFGQKGQLRGCINDVKNMSTYLNQNFGYAREDMVLLTDDQQNPMSQPTKANILRAMHWLVKDAQPNDSLFFHYSGHGGQTPDLDGDEEDGYDEVIYPVDFRQAGHIVDDEMHRIMVRPLRPGVRLTAIFDSCHSGSALDLPYIYSTQGILKEPNLAKEAGQGLLGVVSAYARGDMSGMVSTAVGFLKRATKGDEAYTRSKQTKTSPADVIMWSGSKDSQTSQDAQIGGQATGAMSWAFITALRKNPQQSYVQLLNSIRDELATKYSQKPQLSCSHPQ"
  },
  {
    "reference": "MSVASKNLFDLLGEETPAATTTEKKTAASRDKKRSDSPPVPRELVAQSTTSRKRDPNQPTPRERTVNKKADQPRRRRQAPQGNEAFAREGKEARANNAAHPVDATGAPSNRRNARARRGREFDRHSQTGRVDTKKATERGWGDLVNSAANPDVAENEGNTPSGAQTPAAEEENVKTLDEYLSERKSAAKPVGRTVEKLENATKVEKSAPEELFASLKKSASQKKSAAKESKPKKVLLDIEQTFTARPARGGRPNRAPRRGPSETASKTQQAPPTLSETDFPALA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must have high specificity towards G-quadruplex DNA binding.2. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "QRANQLVQEALEKRWIAELAEYQTVLPEQKYGSENSRIDFLLKADNQPDCFAEVKSTTLLTENDLGMFPDAKTERGQKHLRELAAIAESGQQAVILFAILHTGIQRFAVAKQIDPQYAALFEQAKNVGVKVLAYKAQIR",
    "response#2": "LHNKIAVWLIENGYLLRGYSLEKREPAFGRGRFDLLLKSPEGRRALVEIKGVTLEVGGRALFPDAPTSRGARHMEELARAVAEGYEAHVIFLVLRKRAESMSPHWEMDRKFAESLLRAHKAGVKIHAVKLEI",
    "response#3": "LHNKIAVWLIENGYLLRGYSLEKREPAFGRGRFDLLLKSPEGRRALVEIKGVTLEVGGRALFPDAPTSRGARHMEELARAVAEGYEAHVIFLVLRKRAESMSPHWEMDRKFAESLLRAHKAGVKIHAVKLEL"
  },
  {
    "reference": "MWLRSHRQLCLAFLLVCVLSVIFFLHIHQDSFPHGLGLSILCPDRRLVTPPVAIFCLPGTAMGPNASSSCPQHPASLSGTWTVYPNGRFGNQMGQYATLLALAQLNGRRAFILPAMHAALAPVFRITLPVLAPEVDSRTPWRELQLHDWMSEEYADLRDPFLKLSGFPCSWTFFHHLREQIRREFTLHDHLREEAQSVLGQLRLGRTGDRPRTFVGVHVRRGDYLQVMPQRWKGVVGDSAYLRQAMDWFRARHEAPVFVVTSNGMEWCKENIDTSQGDVTFAGDGQEATPWKDFALLTQCNHTIMTIGTFGFWAAYLAGGDTVYLANFTLPDSEFLKIFKPEAAFLPEWVGINADLSPLWTLAKP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be involved in positive regulation of endothelial cell migration, positive regulation of endothelial cell-matrix adhesion via fibronectin by localizing to Golgi apparatus, Golgi membrane, membrane and exhibiting alpha-(1,2)-fucosyltransferase activity, fucosyltransferase activity.2. The protein design should consider the endogenous proteins and enzymes present in the protein glycosylation pathway to avoid interference with native metabolic processes.3. The protein must exhibit the following characteristics: Preferentially fucosylates soluble lactose and to a lesser extent fucosylates glycolipids gangliosides GA1 and GM1a .. The designed protein sequence is ",
    "response#1": "PLAYVQSDCDPPSDRDSYVQELMKYIAVDSYGECLHNRDLPQQVNNPSSMDDSQFHHILAQYKFILAFENAVCEDYITEKLWRPLKLGAVPVYFGAPNVENWLPSNKSAIIVGRFSHPKELATYIKKLDKNDKLYMQFIEWKLHGNINNKHLLSTITERKWGVQDVTQDNYIDAFECMICKRVWEG",
    "response#2": "PLAYVQSDCDPPSDRDSYVQELMKYIAVDSYGECLHNRDLPQQVNNPSSMDDSQFHHILAQYKFILAFENAVCEDYITEKLWRPLKLGAVPVYFGAPNVENWLPSNKSAIIVGRFSHPKELATYIKKLDKNDKLYMQFIEWKLHGNINNKHLLSTITERKWGVQDVTQDNYIDAFECMICKRVWEES",
    "response#3": "QFFVVMEHCLKHGLKAKKTFLGQNKSFWGPLELVEKLVPEAAEITASVKDLPGLKTPVGRGRAWLRLALMQKKLSEYMKALINKKELLSEFYEPNALMMEEEGAIIAGLLVGLNVIDANFCMS"
  },
  {
    "reference": "MITWLDPQDPFPPVERALGPASDAPGLLAASRDLSPQRLLLAYRQGIFPWYSEGQPVLWWSTDPRMVLAPHRLKISVSLRKTLRRILRDPDWEIRVDDDFVAVMQACAMTPRDGQLGTWITDDIVAAYGSLHRLGLAHSVETWYRGERVGGLYGVALGRMFYGESMFAHRTDASKIALAALCGFLERHGVTMIDCQQETDHLASLGAEPIPREQFIAHVRQTAAEANISPWRFDKSELTRWTSQASTEL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that contributes to protein catabolic process by functioning in cytoplasm with leucyltransferase activity.2. The protein must exhibit the following characteristics: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl- tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine..3. The protein have enhanced an N-terminal L-alpha-aminoacyl-[protein] + L-phenylalanyl-   tRNA(Phe) = an N-terminal L-phenylalanyl-L-alpha-aminoacyl-[protein]   + tRNA(Phe). The designed protein sequence is ",
    "response#1": "MEDKTLGESIEELKAQLARTKEEAEAAIERAYALEKELADTRANLEHQKAASTSTAETDWSVDITPESAEEIVGRNVKALYERIDALEEENDKLRGRTNDRRGN",
    "response#2": "MNASPELSFERVVAALPHRYPFQLVDRIVELDPGKRAVGVKNVTINEPFFQGHFPGHPIMPGVLIIEAMAQVGGFLLLKSMEGTGKIVYFMSADKAKFRKPVVPGDQLVIEAEITKIRGKIGKVKCRAKVDGQLACEAELMFALVD",
    "response#3": "MIKPVFHNDWLYLKSLDPISIGAASAPMPGGVNSPVRAFKAVGGTPPFILSLGDSTIAYGLDGSKKIVIGDCNGNLIKDIVDLVRASGASLSFVADTLVHGQVDITDIAKQAGLRITVVDLHTAGLAKLKDVGGVWVR"
  },
  {
    "reference": "MWLKNLLLLGIVVCSISAPTRPPSPVTRPWRHVNAIKETQILLNQSNDTVAELNETVEVVSENFDPQEPTCLQTRLELYKQGLQGSLARLKGPLTMMASHYKQYCPPTPETSCATQIITFKSFKENLKKFLFDIPFDCWEPSQE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have granulocyte macrophage colony-stimulating factor receptor binding within extracellular space, granulocyte macrophage colony-stimulating factor receptor complex to regulate positive regulation of podosome assembly, macrophage differentiation.2. The protein must exhibit the following characteristics: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.. The designed protein sequence is ",
    "response#1": "CSSQSLQLTSCLDQLHGGLFLYQGLIT",
    "response#2": "MNLLIM",
    "response#3": "CSSQSLQLTSCLDQLHGGLFLYQGLL"
  },
  {
    "reference": "MEAVLSALPWVPVNISGSDLLAKAWFGDSQYRVLLTDLSTVWEEEMSTDDIQSRAQDLNKRLRAPAQAFFSHLCSVARPCFSGLDEDQISAAQAALEQHGESLTVKLKSELAGLPFYWEFRCTTTPVAVVCRQLVRPLLAMTLVLQRQAEDLAALLARKDAEIQDYQENGAVLSRARLQTEPFEVHQYKENFITQILPQMNVTLDSLGFDSELQALYMAVNSGKTGRKRKHSPDSSPAAQENHITDHQHISESTDVGPSLASQEHNNAKESGRSQVANSQQTLPLSSTAGSEDRSTSRAKKKKAVGLFR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: The xrcc4- nhej1/xlf subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other; Plays a key role in NHEJ by promoting the ligation of various mismatched and non-cohesive ends; Associates with xrcc4 to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired; The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors..2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.3. The protein should be optimized for stability and solubility when expressed in nonhomologous end joining complex, site of double-strand break where it will be involved in immunoglobulin V(D)J recombination, double-strand break repair via nonhomologous end joining.4. The designed protein should contain at least one XLM motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "MYNPLKYPNPSLQWHYKVLQAMALDEDVPEALDDATIPKYRQIDKRVGGYLAEWKEALTEKAGGLMKSRAMKREAEDEDVSRPLAKRTLETLHQTRTVDKSEVKKAYKFGGEYIHFKPEEAASLKNLGGKVLRVIGFKPRSLLPTWASVKKSIFIFPSEEHFVGSTPRENANPIMVAIIPSRAMDDETSETPYLPAGLWLYPLPFADDVRNVDLAAPPRPAPNVTISVKGYMPLHRQQPARTCYVWLGGEQAQLAQSETIKVDSATRTVDKSEVKKAYKFGGEYIHFKPEEAASLKNLGGKVLRVIGFKPRSLLPTWASVKKSIFIFPSEEHFVGGAPVADDEQVLGYPRDDDHYVLLEDEELESVALDSTRTIDIESFVPAGSIGWVWYDAPHFLMPDDTIGVEAFAVIRAAMEAAGMVGIARLVLYRRERAVLLEPRGKGIVLWTLHDGNAVRNGGHPAARTLLPLSDLIRYAAAAHMLSDISTAMANGNWKEANELSQCAKSDWNKLKSHPSLRYHENLPLFLRCFTVGWIYTRILSRTVEILQRLHMYEEAVKELESLLSQRVYCPDSRGRWWDRLALNLHQHLKRLEPAIKCITEGLADPEVRTGHRLSLYQRALRLS",
    "response#2": "MYNPLKYPNPSLQWHYKVLQAMALDEDVPEALDDATIPKYRQIDKRVGGYLAEWKEALTEKAGGLMKSRAMKREAEDEDVSRPLAKRTL",
    "response#3": "MAIDLIRYAAAAHMLSDISTAMANGNWKEANELSQCAKSDWNKLKSHPSLRYHENLPLFLRCFTVGWIYTRILSRTVEILQRLHMYEEAVKELESLLSQRVYCPDSRGRWWDRLALNLHQHLKRLEPAIKCITEGLADPEVRTGHRLSLYQRALRLPPETLPQHLSLDLIRYAAAAHMLSDISTAMANGNWKEANELSQCAKSDWNKLKSHPSLRYHENLPLFLRCFTVGWIYTRILSRTVEILQRLHMYEEAVKELESLLSQRVYCPDSRGRWWDRLALNLHQHLKRLEPAIKCITEGLADPEVRTGHRLSLYQRALRLPPETLPQHLSLHQGEVVEYRDINKAFESDDGQMVVITDEDIATLPEERSREIEVVEFIPAEQLDPLMYDKSYFLEPDSKSSKSYVLLAKTLAETDRIAIVHFSLRNKSRLAALRVKDFSKRDVMMIHTLLWPDEIRDPDFPILDKP"
  },
  {
    "reference": "MNRIFRNTIFYLLIFLVIVGIVSFFNGPPTETEEMTYGDFQTRLENREVANITMQPERDVYVIRGQLRTMAEDEFFIVNVPISDIAIEQVLETAKLGDTRLEVLKAEQTSGWVTFFTSIIPFVIIFILFFFLLSQAQGGGSRVMNFGKSKAKLYDDKKKAKFKDVAGADEEKQELVEVVEFLKDPRRFAAIGARIPKGVLLVGPPGTGKTLLARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFENAKKNAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFGVNEGIIIIAATNRADILDPALLRPGRFDRQILVGRPDVKGREEVLKVHARNKPLAEDVNLKTIATRTPGFSGADLENLLNEAALVAARRNKKKVDMNDIEEAIDRVIAGPAKKSRVISEKEKNIVAYHEAGHTVVGVKLESADMVHKVTIVPRGQAGGYAVMLPKEDRYFMTKPELLDKIVGLLGGRVAEEVIFGEVSTGAHNDFQRATGIARKMVTEYGMSDKLGPMQFGTSQGGNVFLGRDINNDQNYSDAIAYEIDQEMQRIIKESYERCRTILTENRDKLELIAKTLMDVETLDAEQIKSLMNDGKLPEKHHSNNNGSTENAEDVKVNIHAKKDEAPPSNAEGEQVTTDNSEDKNEEGNKKE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation.2. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The protein design should emphasize Basic and acidic residues compositional bias.4. The protein must exhibit the following characteristics: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins; Plays a role in the quality control of integral membrane proteins..5. The protein should contain a Helical membrane-spanning region.6. The designed protein should have a high specificity for ATP, Zn(2+) binding, with minimal off-target effects.7. A protein with zinc ion binding, ATP binding, metalloendopeptidase activity, ATP-dependent peptidase activity, ATP hydrolysis activity functions is required to enable cell division, proteolysis, protein catabolic process.8. The protein contains novel AAA+ ATPase domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MVMTEDEKELTAYHEAGHALVGLLVKGNDPLHKVTIIPRGRALGVTMNLPENDKYGYKKSEICARLAMTFGGRVAETLIYGPEDVTTGAGQDIQQATSWARRMVTEWGMSDKLGPLAYSGNEQEVFLGHSVTQTKNISDATAKVIDEEIRRFAGEAEALATEVLTENLDKLHAVAKALLEYETLSGDEVSQRLLEERIARS",
    "response#2": "MVMTEDEKKLTAYHEAGHAVVGLNVPQHDPIHKATIIPRGRALGLVLSLPERDQLSVSWTKYTSKIAMAMGGRVAEELIFGKENITSGASSDIQQATRIARAMVTQFGFADELGYVDYANEQDSHLGSYGGGTSHSQDTQKLIDEKVKEIIDTGYETARRILTEQGQDLENLAQGLLEYETLTGAEIQKLQARLEG",
    "response#3": "MIISEEEKKTTAYHEIGHVLVAKLLPESDPVHKVTIIPRGRALGVTHYLPMDEKHTYSYEYIESKICTLLGGRAAEKIIFNKLTTGAGNDIERATELARKMVCEWGMSEELGPLAYGKNEEEIFLGREVTKQQNYSEMTAQKIDQEVKRIVVGAMEKSEKLLRENLEILHRLSNELLEREILDADEIDKLKALSNIKE"
  },
  {
    "reference": "MPHSQLRIVIVGHVDHGKSTLIGRLLNDTGSLPDGKVEQVREMSRKRGMPFEWAFVMDALQAERDQGITIDTTQIHFKTAQRPYVIIDAPGHAEFLKNMVTGASQADAALLVIDAGEGALEQSRRHAFLLHLLGVRQVAVVVNKMDRVDFDEARYEAVAKEIRDYLGELGLYPKAVIPISARGGENIVTRTDKARWYLGPTVIEQLDAFRPQQTSPDQPLRFPLQDVYKPDDRRILVGRIESGRLRVGDVLRFAPTGADARVVGIEAWNHPQPVLSAHAGQSIGITLDKRIFAERGHIAHHLDAAPRIAHRMAVRLFWLVSEPLVVGKTYTLKIATAEHRVTVEQVTAVIDVEDLTSTPGKAVHRNEVAEVVLRSRTPIAVDLAADLPRTGRGVLIDGNDVVGGFLVVQIEETATANITEVSHAVSVEERAQANGHRGGILWLTGLSGAGKSTLAMALERALFERGWQTFVLDGDNVRNGLNAGLGFSPEDRAENIRRVAETAKLFADAGMVVIASLISPLRADRARARAIGGQRFHEIHVDADLATCEERDPKGLYRRARAGEIAEFTGISAPYEAPEAPELVIDTGRLDRSEALARLLDYVDEALGKATLPGEADRSSSWVI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity.. The designed protein sequence is ",
    "response#1": "KSLLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALESDSKRVGTQGGEIDFALLVDGLAAEREQGITIDVAYRFFATEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSYLAHLIGIRHIVLAVNKMDLVGYDQAIFDSIVADYAAFAESIGIAGFTAIPLSGFKGDNIAAASANTPWYSGPALIEHLESVELDSL",
    "response#2": "DLLRFLTAGSVDDGKSTLIGRLLFDSKRIYDDQLEALQCDSATYGTTGGPDPALLTDGLETERNQGITIDVAYRYFSTDMRSFIICDAPGHEQYTRNMATGASRCDLAIILIDARNGVMPQTKRHSFIASLLGIQHFVVAVNKMDAVDYNHETFRAIRRSYESFAAKLRISNIHFMPISALNGDNVKSLLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALESDSKRVGTQGGEIDFALLVDGLAAEREQGITIDVAYRFFATEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSYLAHLIGIRHIVLAVNKMDLVGYDQAIFDSIVADYAAFAESIGIAGFTAIPLSGFKGDNIAAASANTPWYSGPALIEHLESVELDSLFEAVKAVIPPPALRILACGAVDDGKSTLIGRLLYESGAVPDDQRVALAADSRRYGTADGEIDFALLTDGLEAERERNITIDVAYRYMSTARRDFIIADTPGHEQYTANMATGASQCEVGIILVDAVRGLRPQTFRHAAICSILGIRHVVLAVNKMDGVGFSQERLGQIVEAFAPAAKHLQLDMVAIPISARRGDNVVSISAAMPWYQGVTLLQYLEQLEVPRSFRLPVQWVNRPDLDFRGFAGLIASGTVRPGDAVRVLPSGKTSHVARIVTMDGDLEQAVAGQSVTITLADEIDCSRGDVIASPDNLPEVADQFEATLVWMDEHEMLPGRAYLLKMGTQTMTATVTEPKYEVNVNTLEHMSSKTLGLNAIGVVNISTDRAVPFEAYAKNPDLGGFILIDRMTNATVAAGM",
    "response#3": "ITCGSVDDGKSTLIGRLLFDAKMIFEDQLAALEADSKQSGTTGEAMDLALLVDGLAAEREQGITIDVAYRYFSTDNRKFIIADTPGHEQYTRNMATGASTADLAIILIDARKGVLTQTRRHSYIASLLGIRHVVLAVNKMDLVDYSQTTFDAIRQDYQSFAEKLGFSDITAIPISALKGDNILGSVDDGKSTLIGRLLYDSKLLFEDQLTVLERDSRKHGTAGEEIDFALLLDGLEAEREQGITIDVAYRFFNTEKRKFVVADTPGHEQYTRNMATGASTADLAVILVDARQGVLAQTLRHTYIANMMGIRHVVLAVNKMDLVEHSRSRFHQIVSEYLETADGLDFETITPIPISSPTKETMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLASLENDSKAHGTQGAGIDFALLVDGLAAEREQGITIDVAYRFFATDKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARQGVLTQTRRHSYLVNLLGIRNIVLAVNKMDLVGYSQERFYEIVKDYSHFAQQIGMKSFLPMPISGLAGDNIRILACGAVDDGKSTLIGRLLYESGAVPDDQRVALAADSRRYGTADGEIDFALLTDGLEAERERNITIDVAYRYMSTARRDFIIADTPGHEQYTANMATGASQCEVGIILVDAVRGLRPQTFRHAAICSILGIRHVVLAVNKMDGVGFSQERLGQIVEAFAPAAKHLQLDMVAIPISARRGDNVVSIGSVDDGKSTLIGRLLYDSKMIFEDQLAALESDSKRVGTQGGEIDFALLVDGLAAEREQGITIDVAYRFFATEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSYLAHLIGIRHIVLAVNKMDLVGYDQ"
  },
  {
    "reference": "MDQKSDNAGKCPVAHTVPKGRSNRDWWPDQLDVQVLHQHSGLSDPMGKAFNYAEEFKKLDLEELKKDLHALMTESQDWWPADFGHYGGLFIRMAWHSAGTYRITDGRGGAGQGQQRFAPLNSWPDNANLDKARRLLWPIKQKYGNRISWADLMILTGNVALESMGFQTFGFAGGRADVWEPQELFWGPEGTWLGDERYSGERQLDEPLAAVQMGLIYVNPEGPNGNPDPVAAAREIRETFARMAMNDEETVALIAGGHTFGKTHGAGDPSFIGPDPEGGAIEDQGLGWKSTFGTGVGKDAITGGPEVTWSQTPTRWSNYFFENLFNYEWELTKSPAGAHQWKAKNADASIPDAFDASKKHVPTMLTTDLSLRFDPIYEKISRRFLENPDQFADAFARAWFKLTHRDMGPKVRYLGPEVPAEDLIWQDVIPAVDHKLVDENDVADLKGKVLASGLSVQELVSTAWASASTFRGSDKRGGANGARIRLAPQKDWEVNQPAQLARVLSVLEGIQKDFNTAHTGGKKISLADLIVLAGAAGVEKAAKAGGHDITVPFTPGRADASEAQTDAASFAALEPRADGFRNYVSRRRRQFMKPEEALVDRAQLLTLTAPELTVLVGGLRVVFTSRPEVLTNDFFVNLLDMGTQWSPMAEKEGVYEGRDRKTHEVKWTGTRVDLIFGSHSQLRALAEVYASSDAKEKFVGDFVAAWTKVMNADRFDLV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein with catalase activity, metal ion binding, heme binding is needed to enhance response to oxidative stress, hydrogen peroxide catabolic process in a specific context.2. We need a protein that can perform 2 H2O2 = 2 H2O + O2 with high efficiency.3. The stability of the protein-heme b complex should be optimized to maximize enzymatic activity.4. The protein should be able to bind heme b ligand in a variety of conditions5. For general function, the protein need meet that Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. The designed protein sequence is ",
    "response#1": "LSWGDLMVLAGNVALEDMGFRTVGFAGGRIDAWQPDVVFWGPETKMMGTERSNPDGSLKRSLAATTMGLIYVNPEGPNGNHDPIGAAADIRRAFGQMAMDDEETLALIAGGHTFGKAHGAKKPDQCLDAEPTAAGMEEQGLGWRNKCGKGNAEDTLTSGLEGAWSAAPTQFTSQYIDNLLNFEWVKTKSPAGATQWIPTDPAAAELVPDAHVEGKRHAPIMFTTDIALKEDPGFRKVLESWQKNPELFSKAFARAWFKLTHRDLGPQGRYQ",
    "response#2": "TTNAGAPVWNDNEALTVGPRGPILLEDYHLIEKVAHFARERIPERVVHARGASAKGFFECTHDVTSLTCADFLRAPGVRTPVIVRFSTVIHERGSPETIRDPRGFAVKFYTREGNWDLLGNNFPVFFIRDGIKFPDVIHAFKPNPRSHVQEYWRVFDFLSHLPESLHTFFFLFDDVGVPSDYRHMEGFGVNTYTFVSAAGKAQYVKFHWKPTCGVRCILTDEEAALVGGRNHSHATQDLYDSIAAGSFPEWTLYVQVMDPDTEEQYDFDPLDDTKTWPEDLLPLRPVGRLVLDRNVDNFFNENEQLAFGPGLVVPGIYYSDDKMLQCRVFAYADTQRYRLGPNYLMLPVNAPRCAHHNNHYDGAMNFMHRDEEVDYYPSRHAPLY",
    "response#3": "ISWADLIVLAGTVAYEAAGLKTYGFAFGREDIWHPEKDTYWGEEKEWLAPSDSRYGDVTKAETLANPLAAVQMGLIYVNPEGVNGKSDPQATAYQMRETFARMGMNDEETVALTAGGHTVGKCHGNGNVTDLSPDPEAAGPEFQGLGWMNTKGRGIGRNTMVSGLEGAWTTHPTQWDNGFFEMLFKHEWTLTHSPAGASQWEPITIAEEDKPADVEDPSIRHMPMMTDADMALKVDPIYREISLRFMNDFEAFSDAFARAWFKLTHRDMGPKARYLD"
  },
  {
    "reference": "MYFQNIDALIFSKLLDTLLNIFFLLFFLVLGAEFTLYIINVYFPNLLLYGNALGIKFVLSILGSLIAFPIGLVFQNNYKNFLNYINNFTVDIFFTSLLSIIGALFIANLYFSFLDISSILKEFLFIKYLLRLLIQTILCYSGFILVTFHSDYLLNILVPEMAEAIFSYNKIVRPSITRVLHTSYIINHNIIAIFQSQFVEGLVLIPKSTIQELQILNNSKNFKDKYLGRLGFSRLNELKYNYFDQIILESSLTNCHENVLFKYNKTYNNEQQNIVLMSSCSNNIKESNISQLLISNLFPLIININKLIELLKLPYLSGESFYLQIVRKGKRLGQGIGYLEDGTMVIVNAGSKYIGQKIKVIVKKVWQKSTGKILFTQPIKNS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be expressed specifically in the chloroplast2. The designed protein have TRAM domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "IGGLVAATQLAVKGAKVLVLEKYLIPGGSSGYYERDGFTFDVGSSVMFGFSDKGNLNLITQALAAVGCKMEVIPDPSTVHFHLPSNLSVLVHREYNEFFSELTSKFPHEKEGIFKFYGECWKIFNALNSLELKSLEEPIYLFGQFFKKPMECLTLAYYLPQNAGDIARKFIKDPEVLSFIDAECFIVSTVNALKTPMINASMVLCDRHYGGINYPVGGVGGIAKSLAKGLVDQGSEIQYKANVKSIIVENGKAVGVRLANGNEIFAKNIISNATRWDTFGKLLKQDELPKEEENFQKLYIKAPSFLSIHLGVKSDVLPPDTDCHHFVLEDDWSNLEVPYGSIFLSIPTVLDSSLAPEGNHILHIFTTSSIEDWQGMSQKDYEKKKELVADEIISRLEKKLFPGLQSSIVLKEVGTPKTHRRYLARDSGTYGPMPQGTPKGLLGMPFNTTAIDGLYCVGDSCFPGQGVIAVAFSGVMCAHRAVRTLR",
    "response#2": "IGGLVAATQLAVKGAKVLVLEKYLIPGGSSGYYERDGFTFDVGSSVMFGFSDKGNLNLITQALAAVGCKMEVIPDPSTVHFHLPSNLSVLVHREYNEFFSELTSKFPHEKEGIFKFYGECWKIFNALNSLELKSLEEPIYLFGQFFKKPMECLTLAYYLPQNAGDIARKFIKDPEVLSFIDAECFIVSTVNALKTPMINASMVLCDRHYGGINYPVGGVGGIAKSLAKGLVDQGSEIQYKANVKSIIVENGKAVGVRLANGNEIFAKNIISNATRWDTFGKLLKQDELPKEEENFQKLYIKAPSFLSIHLGVKSDVLPPDTDCHHFVLEDDWSNLEVPYGSIFLSIPTVLDSSLAPEGNHILHIFTTSSIEDWQGMSQKDYEKKKELVADEIISRLEKKLFPGLQSSIVLKEVGTPKTHRRYLARDSGTYGPMPQGTPKGLLGMPFNTTAIDGLYCVGDSCFPGQGVIAVAFSGVMCAHRLRAA",
    "response#3": "IGGLVAATQLAVKGAKVLVLEKYLIPGGSSGYYERDGFTFDVGSSVMFGFSDKGNLNLITQALAAVGCKMEVIPDPSTVHFHLPSNLSVLVHREYNEFFSELTSKFPHEKEGIFKFYGECWKIFNALNSLELKSLEEPIYLFGQFFKKPMECLTLAYYLPQNAGDIARKFIKDPEVLSFIDAECFIVSTVNALKTPMINASMVLCDRHYGGINYPVGGVGGIAKSLAKGLVDQGSEIQYKANVKSIIVENGKAVGVRLANGNEIFAKNIISNATRWDTFGKLLKQDELPKEEENFQKLYIKAPSFLSIHLGVKSDVLPPDTDCHHFVLEDDWSNLEVPYGSIFLSIPTVLDSSLAPEGNHILHIFTTSSIEDWQGMSQKDYEKKKELVADEIISRLEKKLFPGLQSSIVLKEVGTPKTHRRYLARDSGTYGPMPQGTPKGLLGMPFNTTAIDGLYCVGDSCFPGQGVIAVAFSGVMCAHRLARER"
  },
  {
    "reference": "MILYPSVDDLLEQVDSRYSLIMLAAKRAHDLDAGSPELLSNYESPKNIGHALEEIAAGKVKIKED",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Promotes RNA polymerase assembly; Latches the N- and C- terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.2. We need a protein that can perform a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +   RNA(n+1) with high efficiency. The designed protein sequence is ",
    "response#1": "MNRDTAPKV",
    "response#2": "MLVLTRKLNESIKIGDNILITVLGVKGNQVRIGVNAPKEVSVHREEIYQRIQQEK",
    "response#3": "GNFLFTSESVSEGHPDKCCD"
  },
  {
    "reference": "MLFMHIRTRFAPSPTGFIHLGNLRSALYPWAFARHNKGDFILRIEDTDLERSTQEAVDVIIEGMTWLGLDLDEGPIYQMQRIDRYHEVVKQMLDSGLAYPCYMSEEELNLLRDQQMANKEKPHYNGLWRPEPGKMLPPIPEGVKPVIRFKNSIGGSVIWNDAVKGQIEISNDELDDLVIARSDGTPTYNFCVVIDDMDMQITHVIRGDDHVNNTPRQINIMKALGGTPPVYAHLPTVLNDSGEKMSKRNGAMSVRDYQKAGYLPDAILNYLARLGWSHGDAEVFSKEQFVAWFDLESLGRSPAQHNPEKLLWLNHQYIQNSDPDKLAEATKPFAHALGIDTESGPNFVQVVGLLKDRANTLIEIAEGAKLFYMPTPNLSDKQVRENIPETVIPALKDLVGAISSAEPTKEGYAAAFKQVLAQHQLKMPALAMPVRYALFATTQTPAIDSVLVVMGRDEAIKRLSKVIR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Incorporating a 'KMSKS' region, 'HIGH' region motif is critical for the successful design of the protein.2. The ATP binding site should be located in a region of the protein that is accessible to the ligand.3. The protein can be targeted to cytoplasm and perform zinc ion binding, glutamate-tRNA ligase activity, tRNA binding.4. The protein must exhibit the following characteristics: Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).. The designed protein sequence is ",
    "response#1": "MVRLRFAPSPTGYLHVGGARTALFNFLYARKMKGSFILRIEDTDLERSQKEYEEGLINALKWLGLEWDEGPDVGGDFGPYRQSERLEIYWKYARKLIEEGKAYEVYAYPEEIEKLREKLLAQGKAPHYTREMLQQFTTPERIKEYEKKGLKPAIYFEMPRKEYVLNDIVKGRVIFKKGTVGDFAIIRSNGVPIYNFACVIDDHLMNITHVLRGDDHLSNTVKQIALYEAFNWETPEFGHVSMILGPDAKKLSKRHGATSVEEFRDRGYLPQAVVNFLALLGWSHPEGKEIMNLNELIDAFSLDRLGKNPAIFDPKKLRQPEDRAFLQHALDTLPAEPWGEETWKDWTNALKEQSGRKGRSLFMPLRLALTGEDAGPELHVLLGLMGRERTVARLRDALG",
    "response#2": "MVRLRFAPSPTGYLHVGGARTALFNFLYARKMKGSFILRIEDTDLERSQKEYEEGLINALKWLGLEWDEGPDVGGDFGPYRQSERLEIYWKYARKLIEEGKAYEVYAYPEEIEKLREKLLAQGKAPHYTREMLQQFTTPERIKEYEKKGLKPAIYFEMPRKEYVLNDIVKGRVIFKKGTVGDFAIIRSNGVPIYNFACVIDDHLMNITHVLRGDDHLSNTVKQIALYEAFNWETPEFGHVSMILGPDAKKLSKRHGATSVEEFRDRGYLPQAVVNFLALLGWSHPEGKEIMNLNELIDAFSLDRLGKNPAIFDPKKLRWMNAEHFREPEAEFAARVQKWAFNSDYMMKIAPHVQGRVETFSQVAPLGGFFFEGALKLDAKLFESKKLSADQVRQVMQLILWKLESLRQWEKDRITGCIQAVVESLELKLRDAMPLMFAAITGQASSVSVLDAMEILGPDLTRYRLRQAEFAARVQKWAFNSDYMMKIAPHVQGRVETFSQVAPLGGFFFEGALKLDAKLFESKKLSADQVRQVMQLILWKLESLRQWEKDRITGCIQAVVESLELKLRDAMPLMFAAITGQASSVSVLDAMEILGPDLTRYRLRQALEAVG",
    "response#3": "MVRLRFAPSPTGYLHVGGARTALFNFLYARKMKGSFILRIEDTDLERSQKEYEEGLINALKWLGLEWDEGPDVGGDFGPYRQSERLEIYWKYARKLIEEGKAYEVYAYPEEIEKLREKLLAQGKAPHYTREMLQQFTTPERIKEYEKKGLKPAIYFEMPRKEYVLNDIVKGRVIFKKGTVGDFAIIRSNGVPIYNFACVIDDHLMNITHVLRGDDHLSNTVKQIALYEAFNWETPEFGHVSMILGPDAKKLSKRHGATSVEEFRDRGYLPQAVVNFLALLGWSHPEGKEIMNLNELIDAFSLDRLGKNPAIFDPKKLREHPAYIEFAARVQKWAFNSDYMMKIAPHVQGRVETFSQVAPLGGFFFEGALKLDAKLFESKKLSADQVRQVMQLILWKLESLRQWEKDRITGCIQAVVESLELKLRDAMPLMFAAITGQASSVSVLDAMEILGPDLTRYRLRQAIEALG"
  },
  {
    "reference": "MASNSTPKYNSNSLENSLRRSPGDGMNHEQNDEIPCLPGEALITDKDVIYMCPFYGPVKGRIHVTNYKLYFKGEEMEPLISFSVPLGVIARIEKMGGASSRGENSYGLDITCKDMRNLRFALKQEVHSRKQIFEDLTKYAFPLSHGLLLFAFQNEEKFPENGWAVYDAMTEFRRQGLPNGQWRITFINKNYELCDTYPPLLVVPYSASEEDLKKVAAFRSRNRIPVLSWLHPENQSAIMRCSQPLVGMSGKRNKDDERYLDIIRDTNGQTSKLTIYDARPNVNAVANKATGGGYESEDAYPNAELVFLDIHNIHVMRESLKKLKDIVYPNVEESHWLSSLESTHWLEHIKLVLTGAIQVADKVASGKSSVVVHCSDGWDRTAQLTSLAMLMLDSYYRTIVGFEVLVQKEWISFGHKFSSRIGHGDKNHADADRSPIFLQFIDCVWQMSKQFPTAFEFNEHFLITVLDHLYSCRFGTFLYNCENIRDKEKVREKTQSLWSLISSEKSKYTNPFYTKELNRVLYPVASMRHLELWVNYYIRWNPRIRQQQPNPVEQRYMELLALRDDYVRRLEELQITNSPKMNSSTTSPSSPSQIMPQVHTPF",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have a specific function in dephosphorylation and should be localized to ruffle in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "WQMIWQEKSPVVVMVTNVVELGKRKCHKYWPDESAQYGSIKVSQTHIEDLCDYTIRTFVIQQFDGYDVREVKQFHFTAWPDHGVPTRPTNILSFIRRVKSYETPGQGPTTC",
    "response#2": "WQMIWQEKSPVVVMVTNVVELGKRKCHKYWPDESAQYGSIKVSQTHIEDLCDYTIRTFVIQQFDGYDVREVKQFHFTAWPDHGVPTRPTNILSFIRRVKSYETPGQGPTTC",
    "response#3": "WQMIWQEKSPVVVMVTNVVELGKRKCHKYWPDESAQYGSIKVSQTHIEDLCDYTIRTFVIQQFDGYDVREVKQFHFTAWPDHGVPTRPTNILSFIRRVKSYETPGQGPTTV"
  },
  {
    "reference": "MAESLNGLKRTVMCGELRESHIGQKHVVMGWVQRKRNLGGLVFVDLRDREGILQVVFGEEINKEAFEKADLVKPEYCIAVEGELVRRESPNEAMPTGMVELKGQNIKILSESETPPIYIKEDLDTDEAVRLKYRYLDLRRPDMQKIFKVRHKTAKVIRDFLDENGFLEMETPMLTKSTPEGARDYLVPSRNYPGMFYALPQSPQLFKQLLMVSGYDKYFQITKCFRDEDLRANRQPEFTQVDMELSFVDMEDVIALNEKLIQKVFKEVANVDVQLPIQRITYKEAMDKYGSDKPDLRFGMEINDITDAVKDVDFKVFKDAIENGGSVRAIKAPNCATMGRKQIDKLGEFVKTYKAKGLAWIAYKEDEIKSPIAKFLGEEGINKVIESLDAKVGDLILIVADKDSVVLQSLGALRLEMAKRLEILKDNKEFRFAWVTEFPLLSYNEEEDRYQAEHHPFTMPMDEDIEYLESDPGRVRAKAYDIVLNGEELGGGSIRIHDTKLQEKMFNVLGFTSESAWERFGFLLEAFKFGPPPHGGLAYGFDRMIMFLAGTENIKDVIAFPKNQNAYCPLTEAPNVVDEKQLGELGIGVQKQK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be able to bind L-aspartate ligand in a variety of conditions2. The protein design must be focused on enhancing the efficiency of aspartyl-tRNA aminoacylation. The designed protein sequence is ",
    "response#1": "STRLDNRTLDLRKKEVKAVFQIRSEVLRAARDAFRELGCTEINTPKIVATGTEGGTELFPITYFGEEAFMNQSPQLFKQLMIGSGLERVFEIGPIFRAEEHNTPRHLNEATSIDFESAFINHAEAMDACEYVVTAAYQGVAENCITELETLGLRDGFSVPDESFPRISYQEAIERINATGELDEQLVWGDDLPTDGERALGADVGSHYFITDWPSEIKPFYIKDHDDDKTLSTGFDMMHPQMELVSGGQREHRYEHLVSGFEQQGLDPEQFDYYTKMFRYGMPPHAGWGLGGERLVMTMLGLENIREAVLFPRL",
    "response#2": "DEKLRLKFRYLDLRRGRMQKLLRLRHRVMQIMRRHLDERGFTEIETPVLVKSTPEGARDYLVPSRVNPGDWFALPQSPQLFKQLLMVAGFDRYYQIARCFRDEDLRADRQPEFTQLDMEMSFLSMEEIIALNEGLVAAILQETMGLELSLPLPRLTYAEAMVRYGSDKPDTRFGLELVEVSEVFRESSFQVLSGAVAAGGKVVCLPVPGAEGITNTRVKPGGDLFEFVTQFGARGLLFARVREGGQVDTIGAFSKSLTPEIAAGMIEKTGAQPGHLLLFGAGDRTAVPTVLDYMGRLRLKMGEELGLIDPNRHNLLWVTDFPMFEWNAEEKRLEALHHPFTAPRPEDEHDLKTARALAYDIIWNGVEVGGGSLRIYRRALQERVFETIGLTEEEARAKFGFLLDAFEYGTPPHGGIAYGFDRFVMLIAGEQSIREVIAFPKTTG",
    "response#3": "STRLDNRTLDLRKKEVKAVFQIRSEVLRAARDAFRELGCTEINTPKIVATGTEGGTELFPITYFGEEAFMNQSPQLFKQLMIGSGLERVFEIGPIFRAEEHNTPRHLNEATSIDFESAFINHAEAMDACEYVVTAAYQGVAENCITELETLGLRDGFSVPDESFPRISYQEAIERINATGELDEQLVWGDDLPTDGERALGADVGSHYFITDWPSEIKPFYIKDHDDDKTLSTGFDMMHPQMELVSGGQREHRYEHLVSGFEQQGLDPEQFDYYTKMFRYGMPPHAGWGLGGERLVMTMLGLENIREAVLFPRR"
  },
  {
    "reference": "MTRLLGYVDLSEPHFVAAVLAIVFNPLFWNVVARWEHKTRKLSKAFGSPRLACYTLGGAILLLNVLRSHCFTQAMLSQPRMQSLDNPAVYHVGLALLGVGGVFVLSSFLALGFTGTFLGDYFGILKEARVTMFPFSVLDNPMYWGSTAIYLGWAIVHASPTGLLLTALVALIYMVAIVYEEPFTAEIYQQKASQAYKRS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should contain Cytoplasmic, Lumenal topological domains.2. The designed protein should have a specific function within the endoplasmic reticulum membrane. The designed protein sequence is ",
    "response#1": "AWALLQLNEFLLMVILQDKEHTPKLLGYCGDLYITERVPYTSLYGISLPWIIEVFIPMGLRKRMDQWFTPSWPRKAKIVIGLLEFVEDIFHGLYGNFLMCDVSAKNFGYNDKYDLKMVDMRKIIPEMSLKEYIKYKSCESDSDCVYGADCGTTCDQSKRCTTDVTQPNLAKVCLLVKDYLLSGTPSEIRDELEKQIYSCIR",
    "response#2": "AWALLQLNEFLLMVILQDKEHTPKLLGYCGDLYITERVPYTSLYGISLPWIIEVFIPMGLRKRMDQWFTPSWPRKAKIVIGLLEFVEDIFHGLYGNFLMCDVSAKNFGYNDKYDLKMVDMRKIIPEMSLKEYIKYKSCESDSDCVYGADCGTTCDQSKRCTTDVTQPNLAKVCLLVKDYLLSGTPSEIRDELEKQIYSCIL",
    "response#3": "AWALLQLNEFLLMVILQDKEHTPKLLGYCGDLYITERVPYTSLYGISLPWIIEVFIPMGLRKRMDQWFTPSWPRKAKIVIGLLEFVEDIFHGLYGNFLMCDVSAKNFGYNDKYDLKMVDMRKIIPEMSLKEYIKYKSCESDSDCVYGADCGTTCDQSKRCTTDVTQPNLAKVCLLVKDYLLSGTPSEIRDELEKQIYSCIR"
  },
  {
    "reference": "LTETELLSSLTRISSTVVQVNIILTIPAVSLSLPLASDPSLSVSVNPPVAHWGPGPVKVRLISHALRQGQDSVELLALSRPEGPQLSLPGGFSRQTAPLSPILLIHFHGGGFVAQTSKSHESYLKSWSKDLNVPILSVDYSLAPEAPFPRALEECFYAYCWALKNCHLLGSTAERVCLVGESAGGNLCITVSMRAMSHGVRVPDGIVAAYPATLLTTDASPSR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein localizes to the cytosol. The designed protein sequence is ",
    "response#1": "ESEQIKTLNNKFASFIDKVRFLEQQNKLLETKWTLLQEQKSAKSSRLPDIFEAQIAGLRGQLEALQVDGGRLEAELRSMQDVVEDFKNKYEDEINHRTAAENEFVVLKKDVDAAYMSKVELEAKVDALNDEINFLRTLNETELTELQSQISDTSVVLSMDNSRSLDLDGIIAEVKAQYEEMAKCSRAEAEAWYQTKFETLQAQAGKHGDDLRNTRNEISEMNRAIQRLQAEIDNIKNQRAKLEAAIAEAEERGELALKDARAKQEELEAALQRGKQDMARQLREYQELMSVKLALDIEIATYRKLLEGEESRL",
    "response#2": "EESEQIKTLNNKFASFIDKVRFLEQQNKLLETKWTLLQEQKSAKSSRLPDIFEAQIAGLRGQLEALQVDGGRLEAELRSMQDVVEDFKNKYEDEINHRTAAENEFVVLKKDVDAAYMSKVELEAKVDALNDEINFLRTLNETELTELQSQISDTSVVLSMDNSRSLDLDGIIAEVKAQYEEMAKCSRAEAEAWYQTKFETLQAQAGKHGDDLRNTRNEISEMNRAIQRLQAEIDNIKNQRAKLEAAIAEAEERGELALKDARAKQEELEAALQRGKQDMARQLREYQELMSVKLALDIEIATYRKLLEGEESR",
    "response#3": "ESEQIKTLNNKFASFIDKVRFLEQQNKLLETKWTLLQEQKSAKSSRLPDIFEAQIAGLRGQLEALQVDGGRLEAELRSMQDVVEDFKNKYEDEINHRTAAENEFVVLKKDVDAAYMSKVELEAKVDALNDEINFLRTLNETELTELQSQISDTSVVLSMDNSRSLDLDGIIAEVKAQYEEMAKCSRAEAEAWYQTKFETLQAQAGKHGDDLRNTRNEISEMNRAIQRLQAEIDNIKNQRAKLEAAIAEAEERGELALKDARAKQEELEAALQRGKQDMARQLREYQELMSVKLALDIEIATYRKLLEGEESRLN"
  },
  {
    "reference": "MASKRALVILTKGAEEMETIIPVDVMRRAGIKVTVAGLTGKDPVQCSRDVMICPDASLEDAKKEGPYDVVVLPGGNLGAQNLSESAAVKEILKEQENRKGPTALLAHEIGFGSKVATHPLAKDKMMNGSHYTYSENRLKKDGLILTSRGPGTGFEFTLAIVEALNSKEMAAQVKAPLVLKD",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein have DJ-1/PfpI domains with specific sequence motifs that are crucial for its activity.2. A protein with RNA binding is needed to enhance autophagy, single fertilization in a specific context. The designed protein sequence is ",
    "response#1": "LDCSNIKVDGVMWNFGKLGGAHSISETASSHEGYNTTYTLDICKPLTKTLCKKGAFVCAVRNATDINGIERTMDVIDIAGNFVLNSGRTLDPIFTRLKKEDPKTEGLKMELHGGKHKFGNLLKRQKAVITLLCDRERTGLEGLESPKPDGDKKKDGEKKDDDKKDNKDKEGKSKRDGEENKRSLIFKSYNEEEGTLDLEWKTKYACEL",
    "response#2": "LDCSNIKVDGVMWNFGKLGGAHSISETASSHEGYNTTYTLDICKPLTKTLCKKGAFVCAVRNATDINGIERTMDVIDIAGNFVLNSGRTLDPIFTRLKKEDPKTEGLKMELHGGKHKFGNLLKRQKAVITLLCDRERTGLEGLESPKPDGDKKKDGEKKDDDKKDNKDKEGKSKRDGEENKRSLIFKSYNEEEGTLDLEWKTKYACEK",
    "response#3": "LDCSNIKVDGVMWNFGKLGGAHSISETASSHEGYNTTYTLDICKPLTKTLCKKGAFVCAVRNATDINGIERTMDVIDIAGNFVLNSGRTLDPIFTRLKKEDPKTEGLKMELHGGKHKFGNLLKRQKAVITLLCDRERTGLEGLESPKPDGDKKKDGEKKDDDKKDNKDKEGKSKRDGEENKRSLIFKSYNEEEGTLDLEWKTKYACEE"
  },
  {
    "reference": "MDHPERFDVIVVGGGHAGTEAAMAAARIGARTLLLTHSIETVGQMSCNPAIGGIGKGHLVREIDALGGIMARAADRGGIQFRTLNSRKGPAVRATRAQADRQLYRQAIRRAVENQPRLALFQQSVDDLIVEGGRVTGVVTGMGLRFRAPAVVLTVGTFLGGRIHIGESNYGGGRAGDPAANALAARLRELPFQVDRLKTGTPPRLDGRTIDWARLTEQPGDDPAPVFSFLGRPDEHPAQVPCHIAHTRPETHDIIRGALDRSPMYSGTIEGVGPRYCPSIEDKVVRFADKGSHQIFLEPEGLDTHEVYPNGISTSLPFDVQYELVRSIPGLEQARIVRPGYAIEYDFFDPRGLHPTLETRHLEGLWFAGQINGTTGYEEAAAQGLLAGLNAALRVQGREPWYPRRDQAYLGVLVDDLITRGTREPYRMFTSRAEYRLMLREDNADLRLTPIGRDLGLVDDERWRRFNAKREALEREQARLAATLIRPGDLPDALARQVLGGPLRKEQRLEELLRRPDVGYADLMRLPGAGDPVPDAEVVEQLEIQARYAGYLERQHDEVARARRHEQLPLPEGLAYDRVAGLSSEVREKLAAHRPATVGQAARIPGVTPAAVSLLLVHLRRQGLLRESA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must be able to regulate tRNA wobble uridine modification. The designed protein sequence is ",
    "response#1": "MSPAGKLPSEAERILVRKSGAWTNPSYGSYPEKRPILEYIEKGVVNIDKPKGPTSHEVI",
    "response#2": "MSPAGKLPSEAERILVRKSGAWTNPSYGSYPEKRPILEYIEKGVVNIDKPKGPTSHEVL",
    "response#3": "MSPAGKLPSEAERILVRKSGAWTNPSYGSYPEKRPILEYIEKGVVNIDKPKGPTSHEVL"
  },
  {
    "reference": "MNGVGLVGNGLNGTGEPHYEALEGARRPGSPLLSPAAFINPAQYASVLEGRFKQLQDEREAVQKKTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGETLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLLEFETLRKCNAHYNLQNVFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAVEGKRIGKVLDYALEAERLVEKYEVLASELLQWIEQTIATLNDRQLANSLSGVQNQLQSFNSYRTVEKPPKFTEKGNLEVLLFTIQSRLRANNQKVYAPREGRLVSDINKAWERLEKAEHERELALRTELIRQEKLEQLAARFDRKAAMRETWLSENQRLVAQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAELAAERYHDIKRVAARQHNVARLWDFLRQMVAARRERLLINLELQKVFQDLFYLMDWMEEMRGRLRSQDLGKHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFSDPGAGYKPCDPQLVSQRVDALQQSYEGLCELAATRRAQLEESRRLWRFLWEVGEAEAWIREQQHLLASAEPGRDLTGVLRLLSKHAALRDEMSGRLGPLKLTLEQGHQLVTEGHPGAKQAAARGAELQAQWERLEALAEGRARDLAQASSLYQFQAEAHDMETWLV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should contain one or more PH, Calponin-homology (CH) domains that are essential for its function.2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.3. A protein that contributes to vesicle-mediated transport, adult behavior, regulation of postsynaptic specialization assembly by functioning in postsynaptic spectrin-associated cytoskeleton, paranodal junction, cytosol with actin binding, phospholipid binding. The designed protein sequence is ",
    "response#1": "SQQGRLQSPDLGNHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYKPCDPQLVSERVASLERSYEALCKLAAARRARLEESRRLWRFLWEVGEAEAWVREQQHLLASAETGRDLTGVLRLLNKHTALRGEMSGRLGPLKLTPELGHDEFSTQALARQHRALEEEIRSHRPALDALREQAAALPPALSHTPEVQGRVPSLERHYEQLQARSQQGRLQSPDLGNHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYKPCDPQLVSERVASLERSYEALCKLAAARRARLEESRRLWRFLWEVGEAEAWVREQQHLLASAETGRDLTGVLRLLNKHTALRGEMSGRLGPLKLTPELGHDEFSTQALARQHRALEEEIRSHRPALDALREQAAALPPALSHTPEVQGRVPSLERHYEQLQARSLE",
    "response#2": "SQQGRLQSPDLGNHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYKPCDPQLVSERVASLERSYEALCKLAAARRARLEESRRLWRFLWEVGEAEAWVREQQHLLASAETGRDLTGVLRLLNKHTALRGEMSGRLGPLKLTPELGHDEFSTQALARQHRALEEEIRSHRPALDALREQAAALPPALSHTPEVQGRVPSLERHYEQLQARQ",
    "response#3": "SQQGRLQSPDLGNHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYKPCDPQLVSERVASLERSYEALCKLAAARRARLEESRRLWRFLWEVGEAEAWVREQQHLLASAETGRDLTGVLRLLNKHTALRGEMSGRLGPLKLTPELGHDEFSTQALARQHRALEEEIRSHRPALDALREQAAALPPALSHTPEVQGRVPSLERHYEQLQARL"
  },
  {
    "reference": "MTKRPEDTRVVVGMSGGVDSSVAALMLKEQGYDVIGIFMKNWDDTDENGVCTATEDYEDVIRVCNQIGIPYYAVNFEKQYWDKVFQYFLDEYKAGRTPNPDVMCNKEIKFKAFLEHALSLGADYLATGHYARVDRTGDEVKMLRGLDANKDQTYFLNQLTQEQLDKVMFPIGDLEKKRVRELAKEAELATATKKDSTGICFIGERNFKTFLSQYLPAQPGVMQTMDGEVKGEHDGLMYYTIGQRQGLGIGGSGDPWFVVGKDLEQNILFVEQGFHNPLLYSESISAVNISWTRPHIVGENGELTCTAKFRYRQEDHHVKVKMTGEQEAMVIFDEPVRAVTPGQAVVFYDGDECLGGGTIDDVFKDGQKLSYV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must be able to perform tRNA binding under specific conditions.2. The designed protein must have a Cysteine persulfide intermediate, Nucleophile active site that is highly conserved among related enzymes.3. For general function, the protein need meet that Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. The designed protein sequence is ",
    "response#1": "TLSMADLSWTLPDAPAPGEYSAKNRYRMADAACTLSPIVDGQASLTFQEKQWAVTPGQSAVLYHGDICLGGGII",
    "response#2": "DLLRTNRVHWIAEEPPAELVRDKMMECHFRFRHQMALVPCVLTLNQDGSVWVTLVKPARALTPGQFAVFYKGDECLGSGKV",
    "response#3": "SSLTAADLSWISGTPPHQNWVYAAKIRYRQTDAPCAITHFEHDSCQIGFAAPQWGITPGQSVVVYESKVCLGGGVA"
  },
  {
    "reference": "MNLNLRVLTPNRVVWDSPVNEIILSTNSGKIGILPNHASLVTAVDIAVMQIRINSQWSTIVLMGGFAKIDNNELLLLVYDAEKGVDIDPQEAQETFNKAKTNLNKAEGKRQKIEADLAVKKARTRLEAINVLTPTSISN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for proton-transporting ATP synthase complex, catalytic core F(1), chloroplast thylakoid membrane-specific folding and stability, while maintaining ATP binding. The designed protein sequence is ",
    "response#1": "RGSKAGLFGGAGVGKTVLITEMIHNIVSRYDGVSIFCGIGERSREGEELYREMKAAGVLNNTVMVFGQMNEPPGSRFRVGHAALTMAEYFRDQAHRDVLLMIDNVFRFIQAGSEVSGLMGQLPSRVGYQPTLATELAELEERICSTAHGAITSVQAVYIPADDLTDPAAVHTFSHLSASIVLSRKR",
    "response#2": "RGSKAGLFGGAGVGKTVLITEMIHNIVSRYDGVSIFCGIGERSREGEELYREMKAAGVLNNTVMVFGQMNEPPGSRFRVGHAALTMAEYFRDQAHRDVLLMIDNVFRFIQAGSEVSGLMGQLPSRVGYQPTLATELAELEERICSTAHGAITSVQAVYIPADDLTDPAAVHTFSHLSASIVLSRKR",
    "response#3": "RGSKAGLFGGAGVGKTVLITEMIHNIVSRYDGVSIFCGIGERSREGEELYREMKAAGVLNNTVMVFGQMNEPPGSRFRVGHAALTMAEYFRDQAHRDVLLMIDNVFRFIQAGSEVSGLMGQLPSRVGYQPTLATELAELEERICSTAHGAITSVQAVYIPADDLTDPAAVHTFSHLSASIVLSRKR"
  },
  {
    "reference": "MNTIMAATGFLTGYIVPFILVLSLLVFVHEMGHYLVGRWCGIRSTAFSIGFGPELIGFTDKRGTRWKLSAIPLGGYVKFFGDEDAASKSDSSGLSHMSLEERAQTLSGAKLWKRAATVAAGPIANFILAILIFAVLFGIYGRMIADPVVAEVRENSAAATAGVKPGDRLVAIDGEKVMTFEDVRRYVGIRPGTPITVTVERAGEELKLPMVPTRTETTDQFGNKLEMGIIGIVTDQTSGNFRHIEYSPSEAVAEGVRETGHVITGTFNYIGNLVTGRMNADQLGGPVRVAQASGQMATLGISAVIQLAAVLSVSIGLLNLMPVPVLDGGHLVFYAIEAIRGRPLGAGAQEVAFRIGMMMILGLMVFATWNDISSLIG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. Zn(2+) is required for the proper folding and stability of the protein.3. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.4. The protein should have a high affinity for Zn(2+).5. The designed protein have PDZ domains with specific sequence motifs that are crucial for its activity.6. A protein that can perform metalloendopeptidase activity, metal ion binding in plasma membrane to support proteolysis. The designed protein sequence is ",
    "response#1": "IPKGILMVGPPGTGKTLLAKAVAGEANVPFFSISGSDFVEMFVGTGASRVRDMFKTAAKNAPCIVFIDEIDAVGRQRGAGMGGGNDEREQTLNQLLVEMDGMTDNNGIVIVAATNRPDVLDPALMRSGRFDRRVTVNLPYIE",
    "response#2": "VMLSGPPGTGKTLLAKAVAGEAKVPFFSLSGSDFVEMFVGVGASRVRDLFKQAKEKSPCIIFIDEIDAIGRARGKNNFSGGNDERENTLNQLLTEMDGFGTNEHVIVMAATNRADILDRALMRAGRFDRIIYVS",
    "response#3": "LMVGPPGTGKTLLARAIAGEAKVPFFTISGSDFVEMFVGVGASRVRDMFEQAKRHSPCIIFIDEIDAVGRQRGAGLGGGNDEREQTLNQLLVEMDGFEGNEGVIVIAATNRPDVLDAALLRPGRFDRQVTVLS"
  },
  {
    "reference": "MNNNLPSEAVAHAVAVLKNEHVIAYPTEAVFGVGCDPDSETAVMRLLELKQRPVEKGLILIAASFEQLKPYIDDSRLSDSQREAIFSCWPGPVTFVFPARPETPRWLTGRFDSLAVRVTNHPLVIELCEAYGKPLVSTSANLTGQPPCRTTAEVHAQFGDSFPVVDGATGGRQNPSEIRDALTGELFRQG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that localizes to cytoplasm and can perform ATP binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "YSSDANLWHISYEGGVLEDLENEYDESMFKMTVSPEKAPDKPMYLAIAFEQGIPVSIDGNKFTPVELITKLNEIAGANSIGRADIVENRLVGMKSRGVYESPAAAVLYAAHEELESISIDRDTLHFKQLLAHKYAEIAYYGLWFSPLREALDAFINETQKYVTGSIKLKLYKGNIIIVARQAKYSLYSEKLATFEKDEIYNHKDAEGFINLWGLPTKL",
    "response#2": "YSSDANLWHISYEGGVLEDLENEYDESMFKMTVSPEKAPDKPMYLAIAFEQGIPVSIDGNKFTPVELITKLNEIAGANSIGRADIVENRLVGMKSRGVYESPAAAVLYAAHEELESISIDRDTLHFKQLLAHKYAEIAYYGLWFSPLREALDAFINETQKYVTGSIKLKLYKGNIIIVARQAKYSLYSEKLATFEKDEIYNHKDAEGFINLWGLPTKC",
    "response#3": "KPHVNVGTIGHVDHGKTTLTAAITKVLAEAGGAKFRDYASIDNTPEEKARGITINSSHVEYETDARHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGAMPQTREHILLARQMQVPAIVVFLNKVDMLGESDSELLDLVEMELHELLESKGYHNSPIIRGSALRALEGDPKYVESIKQLMKIVDEAVPTPRSD"
  },
  {
    "reference": "MSLAIIILAAGKGTRMKSAKPKVMHTLAGKPMLQHVIDTAKQLSPSQLAVVCGNGADEVVPYLQNQQIDVVMQHEQKGTGHAVEQAKTSFANSEQVLVLYGDVPLITTDTLQDLIESGDNNSLKVLTAILEDPTGYGRIVRDYDDNMLCITEEKDADEETKLINEINTGIMCIPAKWLTEALSQLDNNNAQGEYYLTDLIAKAVNQGIEINSVTCEDEMEVAGINNRVQLAEVESYYQQLKATELMMSGVTFRDPARVDIRGDIKAGQDITIDINVIFEGNNTIADNVSIGANCILINSIIHEGAEILPNSIIENAEVGANCSVGPFARLRPGTKLAAKAKVGNFVEVKNANIGLGSKINHLSYIGDTDMGADVNIGAGTITCNYDGANKHRTVIGDRVFVGSDTQLVAPVTVEDGATIGAGSTIRKTAPADALTLTKSEQKTVKGWQRPVKNKQPFK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity to facilitate UDP-N-acetylglucosamine biosynthetic process.2. The protein should have a low Km value for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate. The designed protein sequence is ",
    "response#1": "MAAGLGTRMKSRRAKVLHLLAGRPLISYVYRTAMALEPSSILIIVGHQAEEVEAIVNRDLEIIAASGRSAPSVKFILQAEQRGTGHAVMQAAPELRGKEGTLMVLSGDVPLVRVEDLLSLISFHKKNGFSATAMTTMLDDPRGYGRILRDSQGQFLRIVEQRDATEEQNRIKEINAGIYCFEIEHLLPALDKLTTANAQGEYYLTDVLEIMKKQGRIGRIVIPEEIVESRKLSGVNTRAELAEIMRILRR",
    "response#2": "MAAGLGTRMKSRRAKVLHLLAGRPLISYVYRTAMALEPSSILIIVGHQAEEVEAIVNRDLEIIAASGRSAPSVKFILQAEQRGTGHAVMQAAPELRGKEGTLMVLSGDVPLVRVEDLLSLISFHKKNGFSATAMTTMLDDPRGYGRILRDSQGQFLRIVEQRDATEEQNRIKEINAGIYCFEIEHLLPALDKLTTANAQGEYYLTDVLEIMKKEGKEINAYVLKDVSEVMGINDRVELALAEKYMRDRINRAHMLKGVSLINPANTYIGVDVKIGSDTVIQPGVMLEGNTVIGEDCHIGPNSRITNSTIGDRTKVDSSVVDSSVVGSDVAVGPFAHIRPATNVGNRAKVGNFVELKKTTLGEGSKASHLTYLGDAEIGTDVNIGCGTITVNYDGVNKFKTIIGDRSFIGCNANLVAPVTINSDTYVAAGTTITKEVPPFALAIGRSRQENKEGYAKKLKDK",
    "response#3": "MAAGLGTRMKSRRAKVLHLLAGRPLISYVYRTAMALEPSSILIIVGHQAEEVEAIVNRDLEIIAASGRSAPSVKFILQAEQRGTGHAVMQAAPELRGKEGTLMVLSGDVPLVRVEDLLSLISFHKKNGFSATAMTTMLDDPRGYGRILRDSQGQFLRIVEQRDATEEQNRIKEINAGIYCFEIEHLLPALDKLTTANAQGEYYLTDVLEIMKKAGGSVKAFGFEDLAEAEAAFQARRRQEMMESGVTLIAPDTVFFSHDTRIAADVVVEPNVVFGPGVNVASNVRIKAFSHLEGASLGEGAEAGPFARLRPGAELGANAKVGNFVEVKKARLGEGAKASHLTYLGDTDVGAHANIGAGTITCNYDGFFKHRTVIGEGAFIGSNTSLVAPVTIGAGAMTGSGSVITEDVPDNALALSRARQTMDINGWAERLRGVSLIAPETVWFSSDTQVSSDVIIEPNVIFGPGVSIASGATIYGHSHIEGATIGANAKVGPFARLRPGAVMEEGSKVGNFVEMKKATLGKGAKANHLTYLGDAEVGEGANIGAGTITCNYDGYFKYKTVIGKGAFIGSNSALVAPVNIGDGAIVGAGATLTKDVAAGDLALVRAEQHEQIVVGPRAAIDPGAELTGPCRILGATTIGQGATIGAFCQITNCVIDAGATVREFCHLEDAHVCAGAVAGPYSRLRPGADVGENARVGNFVEMKKASLGKGAKASHLTYLGDTAVGAGANIGAGTITCNYDGVHKHQTNIGAGAFIGSNTALVAPVQVGENALVAAGSVITKTVPDGALAVARARGVPAR"
  },
  {
    "reference": "MRLQILLIYLICIIVSSIVLVESSNKHHFKENDEVPFYVNNVGPYSNPTETYEFYTLPFCKPSSISYKKTKLGEILQGDSAVLSDYQFPFKSSFENKQLCEYTLKKEDIEKFKKAIGEYYYAEMIYDDLPIFSFVGTVDDSDLTNIRYYLYNHIPFEFDYNGDQVIRVNIDTEHIKVIELSDQDEITLKLTYSAKWQPTEHEFSKRMDLYEEFFTKELEIHWLSVMNSFFLVVLLTAFLAIMIMKILKNDYSRYSKTDEEEDSDYQEDYGWKLVHGDVFRFPPYKNVFSAFYGIGWQFISIVCGILALSLFGMFYPNNGGNMYTAGIVLYALTSGISGYQSAKMYKNMGGNKWAWNIVLTATLFVAPLFIVVILSNTVAITWHSTVALPILTMIEVITIWLFVGFPLTVVGGIAGRRLSENFEAPCRTKNFPREVPPIQWYRRLPCQILIAGFLPFSAIYIELFYIFNSVWGHSTYTLYGILCLVFLILINVTVCITVALTYFQLSMEDHKWWWNSFINGGSTVVFIYMYSIYYYYYISHMYGLLQATFYFTYMLIVCFFFFILLGTVGFYSSLIFVKRIYRNLKSD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Involved in adhesion and phagocytosis of hydrophilic particles..2. A protein that contributes to sorocarp development, protein localization to membrane, phagocytosis, asexual reproduction by functioning in membrane with transmembrane signaling receptor activity.3. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "MLESLAANLLNRLLGSYVENFDPNQLNVGIWSGDVKLKNLKLRKDCLDSLNLPIDVKSGILGDLVLTVPWSSLKNKPVKIIIEDCYLLCSPRSEDHENDEEMIKRAFRLKMRKVSEWELTNQARILSTQSENKTSSSSSEKNNAGFMQSLTTKIIDNLQVTIKNIHLRYEDMDGIFTTGPSSVGLTLNELSAVSTDSNWAPSFIDITQNITHKLLTLNSLCLYWNTDSPPLISDDDQDRSLENFVRGFKDMIASKNSTAPKHQYILKPVSGLGKLSINKLGSTEEQPHIDLQMFYDEFGLELDDTEYNDILHVLSSIQLRQITKKFKKARPSFAVSENPTEWFKYIAACVINEIHEKNKMWTWESMKEKCEQRRLYTKLWVEKLKLKNLEAPLRDPIQEAQLSELHKDLTYDEIILFRSVAKRQYAQYKLGMTEDSPTPTASSNIEPQTSNKSATKNNGSWLSSWWNGKPTEEVDEDLIMTEEQRQELYDAIEFDENEDKGPVLQVPRERVELRVTSLLKKGSFTIRKKKQNLNLGSIIFENCKVDFAQRPDSFLSSFQLNKFSLEDGSPNALYKHIISVRNSSKDQSSIDNHATGEEEEEDEPLLRASFELNPLDGLADSNLNIKLLGMTVFYHVHFITEVHKFFKASNQHMETIGNIVNAAEATVEGWTTQTRMGIESLLEDHKTVNVSLDLQAPLIILPLDPHDWDTPCAIIDAGHMSILSDLVPKEKIV",
    "response#2": "ILYENQRGIMLCGVPYFSEKSLLNFDPAPWVDQHFVASPVSTKTATCPDPSWEWAWQRWYIDMSGDVDESGWQYGFSFTMNNWHGKPVSLHSFVRRRRWIN",
    "response#3": "ILYENQRGIMLCGVPYFSEKSLLNFDPAPWVDQHFVASPVSTKTATCPDPSWEWAWQRWYIDMSGDVDESGWQYGFSFTMNNWHGKPVSLHSFVRRRRWIG"
  },
  {
    "reference": "MAYSYTEKKRIRKSFAELPTVMDIPYLLSIQVDSYEQFLQEHKKPKARENTGLQAAYSSIFPIESHSGNAELQFVEYYLGTPEFDERECILRGSTFAAPMRVKIRLIIKDKDSKDKDSKAAIKDIREQSVYMGEMPLMTANGTFIINGTERVIVSQLHRSPGVFFDHDKGKSHSSGKVLYNARIIPYRGSWLDFEFDAKDLVFARIDRRRKLLASIILRALGLSTSEILDLFFDKVKVYKGEEQFEIDLVADRLRGEMAQFDIVTPEGDVVVEQGKRINARRIRQLEEAGMTKISIPDEYLYERILAEDIIVNDEVIARANTLIDHELLVKLSAFEASESIKEFSILFTNDIDQGSYIADTLRADSTSSREEALIEIYKVMRPGEPPTVETAEKLFDSMFFNADRYDLSNVGRMKFNRRLGLDFVDTDDADIQRERSVLTNADIVNVLKELIEIRNGRGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLSSAESDNLSPQDLINSKPVAAAVKEFFGSSQLSQFMDQNNPLSEVTHKRRVSALGPGGLTRERAGFEVRDVHDTHYGRVCPIETPEGPNIGLINSLATFAKTNSFGFLETPYRRVVDGKVTDVIEYLSAIEEVGTVIAQADSPVTADGALSDEMVSVRSYGEFVRMPPEKVTHMDVSPSQVVSVAAGLIPFLEHDDANRALMGSNMQRQAVPTLRADKPLVGTGMERHVARDSGVCVIAKRGGVIEDVDASRIVVRVNEAEMIAGEAGID",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have DNA-directed 5'-3' RNA polymerase activity within DNA-directed RNA polymerase complex to regulate DNA-templated transcription.2. For general function, the protein need meet that DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The designed protein sequence is ",
    "response#1": "VSIAGVLHEYSALDGVREDVVDILLNLKGVVFKLHGRDSVLLTLKKEGEGAVRASDIDLPHDVEVVNPDHVICHLAAGGKIDMEIKVEKGRGYQPAPARVKQDDNRQIGTILLDASFK",
    "response#2": "NKDSSSKVVSSEFDLIPGVSEDTLEVIANIKNIHLKLGEGEQRKIISFSVSGKDTKVLKASHFERDGVEVFNRDLVIATLSQDVNLDLEFQINYGRGYVSSEQNSKYLEEVNVIALDSIFG",
    "response#3": "RSPGVFYDHDKGKTHSSGKVLYSARVIPYRGSWLDFEFDHKDILYVRIDRRRKMPATVLLKALGNSAEELLNFYYRSP"
  },
  {
    "reference": "MKEFDTIAAISTAIGNSGISIIRVSGKEALSIVDKVFKGKSKKGIIEMNTYTMRYGNIVELSNGDIIDEVIVSFMKGPKSFTGENVVEVNCHGGMYPTKRVLEEIIRAGARLAEPGEFTKRAFLNGRLDLSQAEAVMDIINSKTELSMKSAVAQSEGVISREINKLRQRILEIIAHIEATVDYPEDDLEEVTAENVSKDLNDILKEIDELILSADEGKILREGLNTVIIGKPNVGKSSLLNLLLDEKRAIVTDIPGTTRDVIEEYINISGIPIKIVDTAGIRETEDVIEKMGVERSKEKMENADLIIFMIDSSKKIDAEDLEIIDYIKDKKYIVLLNKVDLKNREDKSKLDLLNKDNIIEFSVKEKVGLEKLKDTIENMFATGNLQHSNTMITNTRHKEALLRAREHCTTSLKALQDTLAIDLASIDIRNAWTALGEITGETLQEDLIDKIFKDFCLGK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein contains novel TrmE-type G domains that confer a unique function or activity.2. The K(+), Mg(2+), GTP, (6S)-5-formyl-5,6,7,8-tetrahydrofolate binding site should be stable and able to withstand changes in environmental conditions.3. The protein should have GTPase activity, metal ion binding, GTP binding and be active in cytoplasm. The designed protein sequence is ",
    "response#1": "LTQAEAIADLINSSSEQAARSALHSLQGDFSKLVNEMVESIIHLRMYVEAAIDFPEEEIDFLADKKIVTDLKAIISRVEDVRKQAQQGSIIREGMRVVIAGRPNAGKSSLLNALSGKQTAIVTDIAGTTRDVLAEQIHIDGMPLHIIDTAGLRDSDDVVEKIGIERAWQEINQADRVLLMVDASEDHSILEDDQDIKDFYPEFFAKLPEKIGLTLIRNKADVNDAKTGFTEFTDTDGTQHAIITLSAKTGKGVDSLKEHLKTIMGYQGGTEGGFMARRRHLVALENTHQHLLTGLDQLESYVAGEILAEELRICQQELDQITGEFTSDDLLGKIFSSFR",
    "response#2": "FKVAIVGKPNVGKSSILNSLLSYERAIISDEAGTTRDRIEENFKVGTHLVRIIDTAGIRKNAGKIEKIGIRYSLAAIEEADIVLAVFDSSSASDEQDERIVELVKNSGKKVFFILNKSDLAFKFDLDLV",
    "response#3": "LAQAEAIADLIDASSEQAARSAINSLQGAFSSHINEMVESLTNLRIYVEAAIDFPDEEIDFLSDGVIEGKLNTVISQLDDVRTQARQGSLLREGMKVVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIEQADRVLFMVDSTTTEATTPEEIWPEFMARLPSTLPVTVIRNKSDLTGEPAEITSQGDYPMIRLSARDGMGIELLRSHLKEAMGFNSNTEGGFLARRRHLQALNTAAEHLQQGYQQLVYAKSGELLAEELRLAQQALSEITGEFTSDDLLGRIFSSFTR"
  },
  {
    "reference": "MTPTHFTISSAFLLGMMGLAFHRTHLLSALLCLEAMMLALFIALSLWSLQLDATGCSTAPMLMLAFSACEASAGLALLVATARTHGTDHMQALNLLQC",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "LAAGLIIGLGAIGACIGIGIMGSKYLEASARQPELMNALQTKMFLLAGLIDAAFLIGVGIAMF",
    "response#2": "ARQPELMNALQTKMFLLAGLIL",
    "response#3": "GHWGS"
  },
  {
    "reference": "MTDSPKTNLLGMTRQQLEGFFTAMGEKPFRAVQVLKWIHQHWVEDFQDMTDLSKALRERLAQVAEIRAPEVVYDQASADGTHKWLLRLDDGNCIETVFIPEKDRGTLCVSSQVGCALDCTFCSTARQGFNRNLSSAEIVGQLWLANRRLAPERTVAGKAPERVVSNVVLMGMGEPLLNFDNVVDAMRLMLDDNAYGLSKRRVTLSTSGIVPAMDRLKETLDVALAVSLHAPNDALRDELVPINRKYPIAELLDACRRYVREERHHQRITFEYVMLEGVNDSPEHARQLIALLRDVPCKINLIPFNPFPETRYRRSGDAAIRRFQEMLANAGYTTITRRTRGDDIDAACGQLVGKVADRSRRALRFARLEGTGGTAP",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have at least one Radical SAM core domain.2. The designed protein must possess rRNA binding, metal ion binding, tRNA binding, 4 iron, 4 sulfur cluster binding, tRNA (adenine-C2-)-methyltransferase activity to support rRNA base methylation under certain conditions.3. The protein must exhibit the following characteristics: m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.4. The protein must be engineered to have a high affinity for [4Fe-4S] cluster in order to increase its catalytic activity.5. The Proton acceptor, S-methylcysteine intermediate active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.6. The protein should be able to bind [4Fe-4S] cluster ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "EEGRGTLCVSSQVGCTLTCSFCHTGTQKLVRNLTSGEILSQLILARHRLGDFPDVEAAPGTMVPSEGRKVSNIVMMGMGEPLYNFENVKQALLIASDGEGLSISRRRITLSTSGVVPEIRRAGEEIGVMLAISLHAVRDDLRDELVPINKKYPLKDLLQACRDYPGLSNARRITFEYVMLKGVNDSLEDARDLVKLLKGIPAKINLIPFNPWPGSAYECSDWEQIERFADFVNHAGYASPIRTPRGRDIDAACGQLAGQRKRTG",
    "response#2": "EDGRGTLCVSSQAGCTLDCAFCSTGKQGFNRNLSAAEIIGQLWLANRALGIHAGGARAISNVVFMGMGEPMLNLDAVIPAARLMIDDNGYGLSRRRVTVSTSGVVPGMDRLAGECPVALAVSLHAADDALRDRLVPINRKYPIRQLLAACIRYLERAPRDFITFEYVMLDGVNDSDAQAKALLGIASQVRCKFNLIPFNPFPDSGFKRSSPERIRRFAEVLQRAGLTVTTRKTRGEGIAACRQAIGELTKTSSQVGCALDCRFCATAQQGFNRNLTLGEIIAQLWLAEHELRAEGYQLDESYARVISNVVMMGMGEPLANFNHVVKAMKLMMDDFAYGLSWRRITLSTAGIVPALLRLKEECPVNLAVSLHATNDELRNQLVPINQKYPIAELLAACRAYVEEDNRRRVTFEYVMLKGINDREAC",
    "response#3": "EDGRGTLCVSSQAGCTLDCAFCSTGKQGFNRNLSAAEIIGQLWLANRALGIHAGGARAISNVVFMGMGEPMLNLDAVIPAARLMIDDNGYGLSRRRVTVSTSGVVPGMDRLAGECPVALAVSLHAADDALRDRLVPINRKYPIRQLLAACIRYLERAPRDFITFEYVMLDGVNDSDAQAKALLGIASQVRCKFNLIPFNPFPDSGFKRSSPERIRRFAEVLQRAGLTVTTRKTRGEGIDASAAVGRISLIGMTLAEMEEAVTSLGLPKFRAKQIYGWVYQKGVRDVEEMSNVPKDLRAVLAEKYSVERPKIITDQKSEDGTHKWLLGMSDGQQVETVFIPESDRGALCVSSQVGCTLTCRFCHTGTQPLVRNLGAWEIISQIMHTRDALEEWPSSAAMPRLFTNVVMMGMGEPLYNYHNVAKALKIVMDGDGLAISKRKITLSTSGVVPAIYKCGEELGVNLAISLHAVTNELRSQIMPINNKYPLEELLQACRDYPGASPSRRITFEYVMLKGVNDSIADAKELVKILKNIHAKVNLIPFNDWPGSGFECSDWQTITNFGRILEEAGYESPIRKPRGRDILAACGQLKSASKRAHTKDG"
  },
  {
    "reference": "MFFLAAEGGETSHSPILPVWQEIVVGLVAFGLLAFVLMKFVFPRMEQTFQARVDAIEGGIKRAEAAQAEANQLLEQYRAQLSEARSDAAKIRDDARADAEGIRQDILAKAREESDRIIAAGKEQLVAERATIVRELRTEVGTLAVDLASKIVGESLADEARRAGTVDRFLDGLESAGAR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein can be targeted to plasma membrane, proton-transporting ATP synthase complex, coupling factor F(o) and perform proton-transporting ATP synthase activity, rotational mechanism. The designed protein sequence is ",
    "response#1": "GQRVFDTFFPLGMGGTAAIPGGFGAGKTMTQHQLAKWSSADIIVYVGCGERGNEMTDVLEEFPKLEDPSTGKSMMERTVLIANTSNMPVAAREASIYTGITIAEFYRDMGYNVALMADSTSRWAEALREISGRLEEMPAEEGFPAYLPSRLAEFYERAGYVKTLGQDREGSISLIGAVSPPGGDFSEPVTQNTKRYVRCFWALDKSLASARHFPAVNWLESYV",
    "response#2": "VNPLDRDREWTVSVKVKLGDRVKPGQVVAEVPETSIVTHRVMVPPHLSGEVVEVVPNGKYTVDQEIVTVKDDKGNNHQIRLHQKWPVK",
    "response#3": "VNPLDRDREWTVSVKVKLGDRVKPGQVVAEVPETSIVTHRVMVPPHLSGEVVEVVPNGKYTVDQEIVTVKDDKGNNHQIRLHQKWPVR"
  },
  {
    "reference": "MLCALLYVRYLFLPILSFQRFDPPEELERKVSELADLIKASSHVVFHTGAGISTASGIPDFRGPNGVWTMEERGLAPKFDTTFENARPSKTHMALIELERVGILKFLVSQNVDGLHVRSGFPRDKLAELHGNMFVEECMKCGKQYVRDTAVGSMGLKPTGRLCDVPKTRGLRACRGKLVDTILDWEDALPDRDLALADEACRKADLSITLGTSLQIKPSGNLPALTKKSGGKLVIVNLQPTKHDKQAILRIHGYLDEVVTKLMKHLGLEIPEWRGPVVVEESSALAVKPEPPPPLLLKLERDIKYPAKKGLHAHCNGTADGLGIAVKKESRGPDRDPGATKRAKVEPLLT",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be involved in determination of adult lifespan by localizing to pericentric heterochromatin and exhibiting lncRNA binding. The designed protein sequence is ",
    "response#1": "WDNLGVGMKVEVENTDCDSIEVIQPGQTPTSFWVATILEIKGYKALMSYEGFDTDSHDFWVNLCNAEVHSVGWCATRGKPLIPPRTIEHKYKDWKDFLVGRLSGARTLI",
    "response#2": "PAATPVPIPIGWQRCVREGAVYYISPSGTELSSLEQTRSYLLSDGTCKCGLECPLNVPKVFNFDPLAPVTPGGAK",
    "response#3": "TKIREIQQDGNVYSRLASSIAPEIFGLLDVKKTLLLLLVGGVTKELGDGMKIRGDINVCLMGDPGVAKSQLLKSITHIAPRGVYTTGKGSSGVGLTAAVMRDPITDEMILEGGALVLADNGICSIDEFDKMDENDRTAIHEVMEQQTISISKAGITTTLNARTSILAAANPLHGRYNTRISPHENINLPAALLSRFDVIFLLLDKPSRDADERLAEHVAYVL"
  },
  {
    "reference": "MHSFLLATAVPATLSWSPKVAGVMIACNILAIAFGKLTIKQQNVGTPMPSSNFFGGFGLGAVLGTASFGHILGAGVILGLANMGVL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have a specific function within the photosystem I.2. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "IKPYNDDPFVGHLATPITSSSLTRALLKNLPAYRFGLTPLLRGLEIGLAHGYFLIGPFAQLGPLRNSDIGLLAGFLSTIGLILILTLGLTIYGAAAFGQEKSNGSELQTKKSWDQFKGGFFVGACGSAGFAFICLSSL",
    "response#2": "IKPYNDDPFVGHLATPITSSSLTRALLKNLPAYRFGLTPLLRGLEIGLAHGYFLIGPFAQLGPLRNSDIGLLAGFLSTIGLILILTLGLTIYGAAAFGQEKSNGSELQTKKSWDQFKGGFFVGACGSAGFAFICLSSL",
    "response#3": "IKPYNDDPFVGHLATPITSSSLTRALLKNLPAYRFGLTPLLRGLEIGLAHGYFLIGPFAQLGPLRNSDIGLLAGFLSTIGLILILTLGLTIYGAAAFGQEKSNGSELQTKKSWDQFKGGFFVGACGSAGFAFICLSSV"
  },
  {
    "reference": "GFLRARTTSVDRPAAVRGPRNKKRGWRRLAQEAAGFLEDVRPTERTSGVREAPNEKLFFVDTGSKEKGLTKKRTKVQKKSLLLRKPLRVDVILENTSKVPAPKNVLAHQVPNAKKLRRKEQLWEKLAKQGELPREVRRARARLLNPPAAKAKPRPQDTVERPFYDLWAPDNPLDRPLVGQDEFFLEQTKKKGVKWSLGLGTLPSQWPGTPARDWSSLPASRPSQTLLSAAHEVELQRQKEAEKLERQLALPAAEQAATQESTFQELCEGLLEESDGEGEPGQGEELEAGDAEVCPMPARLATTEKKTEQQRRREKAARRLRAQQAVLRAARLRHQELFRLRGIKAQVALRLAELARRRKQRQARREAEADKPRRLGRLKYQAPDIDVQLSSELTDSLRTLKPEGNILRDRFKSFQRRNMIEPRERAKFKRKYKVKLVEKRAFREIQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be optimized for stability and solubility when expressed in rDNA heterochromatin where it will be involved in negative regulation of signal transduction by p53 class mediator. The designed protein sequence is ",
    "response#1": "AHYWKDFHPRWPFLHRGTLKVDEAPVELLLAMITMGMHFVGDAFAYSIAVSIHSTLRFSIYTHPDFKPPASLWVYQALLIAEIFEKMTSTVEQHNLSQIFHGVTIESMQNGLSSKDTVTEKIPKNMTGTNIAQHKWHQWVDREASKRIAFFSFVLDSQHVILFGYRPLIDITSVGLPLLCDEALWNADSYEAWTSLLNENDPPHFFPVLKMFLTNEHLPPKLSPWNMMIVLHGLTTIGWILSRENLGIVENIMQNNGTNLKNWRILLKASYKFV",
    "response#2": "LHCVQTIFVILSGQGEVLNIDPIRFYQHFYRNMLAVQAGKNHDDFAIILRTLDEVLVKRRRNMSQQRLMAFMKRLLTGSLHLLHNGTLATLGTIKQTFQLTSVLDNLLDTDTTIGSGRYDPELDDPEYCNAASTALYELALLARHYHPTVRRMIG",
    "response#3": "LHCVQTIFVILSGQGEVLNIDPIRFYQHFYRNMLAVQAGKNHDDFAIILRTLDEVLVKRRRNMSQQRLMAFMKRLLTGSLHLLHNGTLATLGTIKQTFQLTSVLDNLLDTDTTIGSGRYDPELDDPEYCNAASTALYELALLARHYHPTVRRML"
  },
  {
    "reference": "MGPLHQFLLLLITALSQALNTTVLQGMAGQSLRVSCTYDALKHWGRRKAWCRQLGEEGPCQRVVSTHGVWLLAFLKKRNGSTVIADDTLAGTVTITLKNLQAGDAGLYQCQSLRGREAEVLQKVLVEVLEDPLDDQDAGDLWVPEESSSFEGAQVEHSTSRNQETSFPPTSILLLLACVLLSKFLAASILWAVARGRQKPGTPVVRGLDCGQDAGHQLQILTGPGGT",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should contain Extracellular topological domains.2. The designed protein should have amyloid-beta binding, protein tyrosine kinase binding, phospholipid binding, transmembrane signaling receptor activity, low-density lipoprotein particle binding within membrane, plasma membrane raft, extracellular region, plasma membrane to regulate negative regulation of autophagic cell death, microglial cell proliferation, positive regulation of osteoclast differentiation, negative regulation of autophagy, positive regulation of peptidyl-tyrosine phosphorylation. The designed protein sequence is ",
    "response#1": "GNSVAIWMFVFHMKPWSGISVYMFNLALADFLYVLTLPALIFYYFNKTDWIFGDVMCKLQRFIFHVNLYGSILFLTCISAHRYSGVVYPLKSLGRLKKKNAIYVSVLVWLIVVVAISPILFYSGTGIRKNKTVTCYDSTSDEYLRSYFIYSMCTTVAMFCIPLVLILGCYGLIVRALIYKDLDNSPLRRKSIYLVIIVLTVFAVSYIPFHVMKTMNLRARLDFQTPEMCDFNDRVYATYQVTRGLASLNSCVDPILYYFVSHTCGPAHFRCNSSSCVPALWACDGEPDCDDGSDEWPARCRRTCPPIPPRLPDFSAEYSLPFFRADKPVQNIAVTAYTPPQIFVGHQNAIVALNDSMQKIWEVKTGPLGSPDCGTCHICHTEPDPNDHVDTDNQVLLMDPAVFLPYLYMCGSTQYGICYFIDISSPQPEPKCLYREKLNFKDNCPDCLASPLGTKVTIVEYGATSYFFIAATVNDRVAQNYPRRSISVARPLSTEDGFDMVMSGLTVLPELRQSYAIDYIYTFSTEEYVYFLSLQRENPSNRNSAFQTRLGRLPISIPEVWMYREVVLECRYNPKRRRRRRENPKDIVYNGLQAAHFGRAGLDLAQELRVDTKEDILYGVFAEVNERGEAKKNSAFCAFPLLKVNHAIEMGVEACCKSGTEQLSRGLCHYQLCENCPHESSWDNYTCSNKPTLVSKPHYRVDLFNNRMKGVLFTAVLVNIIGNHTIGHFGTSDGRIMQVILTQYKPNFFSNFSLGNTEVSRTATVHSEDYLLFVAGNKLFKVP",
    "response#2": "GNSVAIWMFVFHMKPWSGISVYMFNLALADFLYVLTLPALIFYYFNKTDWIFGDVMCKLQRFIFHVNLYGSILFLTCISAHRYSGVVYPLKSLGRLKKKNAIYVSVLVWLIVVVAISPILFYSGTGIRKNKTVTCYDSTSDEYLRSYFIYSMCTTVAMFCIPLVLILGCYGLIVRALIYKDLDNSPLRRKSIYLVIIVLTVFAVSYIPFHVMKTMNLRARLDFQTPEMCDFNDRVYATYQVTRGLASLNSCVDPILYCWLNENFKVALSRLFSERTCPPIPPRLPDFSAEYSLPFFRADKPVQNIAVTAYTPPQIFVGHQNAIVALNDSMQKIWEVKTGPLGSPDCGTCHICHTEPDPNDHVDTDNQVLLMDPAVFLPYLYMCGSTQYGICYFIDISSPQPEPKCLYREKLNFKDNCPDCLASPLGTKVTIVEYGATSYFFIAATVNDRVAQNYPRRSISVARPLSTEDGFDMVMSGLTVLPELRQSYAIDYIYTFSTEEYVYFLSLQRENPSNRNSAFQTRLGRLPISIPEVWMYREVVLECRYNPKRRRRRRENPKDIVYNGLQAAHFGRAGLDLAQELRVDTKEDILYGVFAEVNERGEAKKNSAFCAFPLLKVNHAIEMGVEACCKSGTEQLSRGLCHYQLCENCPHESSWDNYTCSNKPTLVSKPHYRVDLFNNRMKGVLFTAVLVNIIGNHTIGHFGTSDGRIMQVILTQYKPNFFSNFSLGNTEVSRTATVHSEDYLLFVAGNKLFKVP",
    "response#3": "RTCPPIPPRLPDFSAEYSLPFFRADKPVQNIAVTAYTPPQIFVGHQNAIVALNDSMQKIWEVKTGPLGSPDCGTCHICHTEPDPNDHVDTDNQVLLMDPAVFLPYLYMCGSTQYGICYFIDISSPQPEPKCLYREKLNFKDNCPDCLASPLGTKVTIVEYGATSYFFIAATVNDRVAQNYPRRSISVARPLSTEDGFDMVMSGLTVLPELRQSYAIDYIYTFSTEEYVYFLSLQRENPSNRNSAFQTRLGRLPISIPEVWMYREVVLECRYNPKRRRRRRENPKDIVYNGLQAAHFGRAGLDLAQELRVDTKEDILYGVFAEVNERGEAKKNSAFCAFPLLKVNHAIEMGVEACCKSGTEQLSRGLCHYQLCENCPHESSWDNYTCSNKPTLVSKPHYRVDLFNNRMKGVLFTAVLVNIIGNHTIGHFGTSDGRIMQVILTQYKPNFFSNFSLGNTEVSRTATVHSEDYLLFVAGNKLFKVPS"
  },
  {
    "reference": "MKAVSPVRPQSRKAQVPAVCGELALHCLSEHSLGVARYKMEEEETLCLQYDMNDCYSRLKRLVPTIPPNKKVSKVEILQHVIDYILDLQLALDTHPVLLRQQPPTRTPLTDLNTDPAALVVNKQGDSILCR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have bHLH transcription factor binding, protein dimerization activity within nucleus, cytoplasm to regulate negative regulation of transcription by RNA polymerase II.2. The designed protein should contain one or more bHLH domains that are essential for its function.3. For general function, the protein need meet that Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity; Inhibits the activity of both neurogenic (neurog1/neurogenin, neurod1/neuroD) and myogenic (myod1/myoD) bHLH factors . The designed protein sequence is ",
    "response#1": "MPGMICKNSDLEFDSLKPCFYPEDDDIYFGGRNSTPPGEDIWKKFELLPTPRLSPGRALAEDSLEPANWATEMLLPEADLWSNPAEEEDIFGLKGLSGSSSNPVVLQDCMWSGFSSREKPETVVSEKLPGGCGSLAVGAGTLVPGAAAATSAGHARSGTAGVGRRKAAWLTELSHLDSECVDSAVIFPANKRESMPVATIPASAGAAISLGDHQGLSSSLEDFLSNSGYVEEGGEEIYVVMLGETQFSKTVTKLPTAAHSENAALTPECAQSGELILKRSDLIQEQHNYAAPPLPYAEDARPLKKPRSQDPLGPLKCVLRPA",
    "response#2": "MPGMICKNSDLEFDSLKPCFYPEDDDIYFGGRNSTPPGEDIWKKFELLPTPRLSPGRALAEDSLEPANWATEMLLPEADLWSNPAEEEDIFGLKGLSGSSSNPVVLQDCMWSGFSSREKPETVVSEKLPGGCGSLAVGAGTLVPGAAAATSAGHARSGTAGVGRRKAAWLTELSHLDSECVDSAVIFPANKRESMPVATIPASAGAAISLGDHQGLSSSLEDFLSNSGYVEEGGEEIYVVMLGETQFSKTVTKLPTAAHSENAALTPECAQSGELILKRSDLIQEQHNYAAPPLPYAEDARPLKKPRSQDPLGPLKCVLRPGS",
    "response#3": "MPGMICKNSDLEFDSLKPCFYPEDDDIYFGGRNSTPPGEDIWKKFELLPTPRLSPGRALAEDSLEPANWATEMLLPEADLWSNPAEEEDIFGLKGLSGSSSNPVVLQDCMWSGFSSREKPETVVSEKLPGGCGSLAVGAGTLVPGAAAATSAGHARSGTAGVGRRKAAWLTELSHLDSECVDSAVIFPANKRESMPVATIPASAGAAISLGDHQGLSSSLEDFLSNSGYVEEGGEEIYVVMLGETQFSKTVTKLPTAAHSENAALTPECAQSGELILKRSDLIQEQHNYAAPPLPYAEDARPLKKPRSQDPLGPLKCVLRPQ"
  },
  {
    "reference": "MELWRQCTHWLIQCRVLPPSHRVTWDGAQVCELAQALRDGVLLCQLLNNLLPHAINLREVNLRPQMSQFLCLKNIRTFLSTCCEKFGLKRSELFEAFDLFDVQDFGKVIYTLSALSWTPIAQNRGIMPFPTEEESVGDEDIYSGLSDQIDDTVEEDEDLYDCVENEEAEGDEIYEDLMRSEPVSMPPKMTEYDKRCCCLREIQQTEEKYTDTLGSIQQHFLKPLQRFLKPQDIEIIFINIEDLLRVHTHFLKEMKEALGTPGAANLYQVFIKYKERFLVYGRYCSQVESASKHLDRVAAAREDVQMKLEECSQRANNGRFTLRDLLMVPMQRVLKYHLLLQELVKHTQEAMEKENLRLALDAMRDLAQCVNEVKRDNETLRQITNFQLSIENLDQSLAHYGRPKIDGELKITSVERRSKMDRYAFLLDKALLICKRRGDSYDLKDFVNLHSFQVRDDSSGDRDNKKWSHMFLLIEDQGAQGYELFFKTRELKKKWMEQFEMAISNIYPENATANGHDFQMFSFEETTSCKACQMLLRGTFYQGYRCHRCRASAHKECLGRVPPCGRHGQDFPGTMKKDKLHRRAQDKKRNELGLPKMEVFQEYYGLPPPPGAIGPFLRLNPGDIVELTKAEAEQNWWEGRNTSTNEIGWFPCNRVKPYVHGPPQDLSVHLWYAGPMERAGAESILANRSDGTFLVRQRVKDAAEFAISIKYNVEVKHIKIMTAEGLYRITEKKAFRGLTELVEFYQQNSLKDCFKSLDTTLQFPFKEP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The target protein must exhibit helix-turn-helix-helix-helix-helix-turn-helix-helix-helix-turn-helix-helix-helix-helix as its primary conformation.2. The designed protein should contain one or more DH, SH2 domains that are essential for its function.3. The designed protein should be involved in immune response, T cell differentiation by localizing to plasma membrane and exhibiting metal ion binding, phosphorylation-dependent protein binding. The designed protein sequence is ",
    "response#1": "TSHNPKDYADAFAVRQKFDRNLQTGKQFKFETVCGLFLLKGVDKITPGVPAKVLKATSKLADLEDIFGVSPFARKYRELLKTACQWSLTVETLDARAQTLDEIFDPTEILWLQVAAKIQVSAMAMRRLVGEVTAKVMDALGSNMSALFQIFKQQIVRIFQKALAIFENVSELPQRIAALKMAFAKCAKSITVVVMERTLVVREFAGTCLASINGAVAKFFEELPNGFMGAKIFTTLAFFREAAVKIVDNIPNAPRGTKGFEVVGNAKGTQVVVRGMRNDLTLLDQKAEIPVESEGWSAILGGHLCYVFKSGDRFYAAPLSGNFALHDVHCCERVVV",
    "response#2": "TSHNPKDYADAFAVRQKFDRNLQTGKQFKFETVCGLFLLKGVDKITPGVPAKVLKATSKLADLEDIFGVSPFARKYRELLKTACQWSLTVETLDARAQTLDEIFDPTEILWLQVAAKIQVSAMAMRRLVGEVTAKVMDALGSNMSALFQIFKQQIVRIFQKALAIFENVSELPQRIAALKMAFAKCAKSITVVVMERTLVVREFAGTCLASINGAVAKFFEELPNGFMGAKIFTTLAFFREAAVKIVDNIPNAPRGTKGFEVVGNAKGTQVVVRGMRNDLTLLDQKAEIPVESEGWSAILGGHLCYVFKSGDRFYAAPLSGNFALHDVHCCERVVI",
    "response#3": "RDVILVSKDIPEQLCDALFFYTSHNPKDYADAFAVRQKFDRNLQTGKQFKFETVCGLFLLKGVDKITPGVPAKVLKATSKLADLEDIFGVSPFARKYRELLKTACQWSLTVETLDARAQTLDEIFDPTEILWLQVAAKIQVSAMAMRRLVGEVTAKVMDALGSNMSALFQIFKQQIVRIFQKALAIFENVSELPQRIAALKMAFAKCAKSITVVVMERTLVVREFAGTCLASINGAVAKFFEELPNGFMGAKIFTTLAFFREAAVKIVDNIPNAPRGTKGFEVVGNAKGTQVVVRGMRNDLTLLDQKAEIPVESEGWSAILGGHLCYVFKSGDRFYAAPLSGNFALHDVHCCERVVCS"
  },
  {
    "reference": "MKLALALSLIASVAAAPTATLANGDTITGLNAIINEAFLGIPFAEPPVGNLRFKDPVPYRGSLNGQSFTAYGPSCMQQNPEGTYEENLPKVALDLVMQSKVFQAVLPNSEDCLTINVVRPPGTKAGANLPVMLWIFGGGFEIGSPTIFPPAQMVSKSVLMGKPIIHVAVNYRLASFGFLAGPDIKAEGSSNAGLKDQRLGMQWVADNIAGFGGDPSKVTIFGESAGSMSVLCHLLWNGGDNTYKGKPLFRAGIMQSGAMVPSDPVDGTYGTQIYDTLVASTGCSSASNKLACLRGLSTQALLDATNDTPGFLSYTSLRLSYLPRPDGANITDDMYKLVRDGKYASVPVIIGDQNDEGFLFGLSSLNTTTEADAEAYLRKSFIHATDADITALKAAYPSDVTQGSPFDTGILNALTPQLKRINAVLGDLTFTLSRRYFLNHYTGGPKYSFLSKQLSGLPILGTFHANDIVWQHFLLGSGSVIYNNAFIAFATDLDPNTAGLSVQWPKSTSSSQAGDNLMQISALGLYTGKDNFRTAGYNALFADPSHFFV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Charge relay system active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "GGDAIYGGGSRCSAAFNVTKNGVRYFLTAGHCTNLSSTWSSTSGGTSIGVREGTSFPTNDYGIVRYTTTTNVDGRVNLYNGGYQDIASAADAVVGQAIKKSGSTTKVTSGTVSAVNVTVNYSDGPVYGMVRTTACSAGGDSGGAHFAGSVALGIHSGSSGCTGTNGSAIHQPVREC",
    "response#2": "GGDAIYGGGSRCSAAFNVTKNGVRYFLTAGHCTNLSSTWSSTSGGTSIGVREGTSFPTNDYGIVRYTTTTNVDGRVNLYNGGYQDIASAADAVVGQAIKKSGSTTKVTSGTVSAVNVTVNYSDGPVYGMVRTTACSAGGDSGGAHFAGSVALGIHSGSSGCTGTNGSAIHQPVREC",
    "response#3": "IIGGFKCKKNSQPWHVAVLRSNKYICGGVLLDPNWVLTAAHCYGNDTSQHNVWLGKNKLFQREPSAQHRWVSKSFPHPDYNMSLLNDHIPHPEDKSNDLMLLRLSKPADITDAVKPIDLPTEEPKLGSTCLASGWGSITPTKYQIPNDLQCVFIKLLPNENCAKAYVHKVTDVMLCVGETGGGKGTCKGDSGGPLICDGVLHGITSWGSIPCAKPNAPGVFTKLIKFTSWIK"
  },
  {
    "reference": "MRFDLEPPSSVAAAHRTGVLLINLGTPDAPTPRAVRRYLAEFLSDPRVVEIPQVVWQVLLRTLILPLRGRASAKKYAAVWMPEGSPLRVYTERQTEGVRHLLASNAYQVTVDYAMRYGSPNIAQALAQFKRAGVERVLLMPMYPQYSASTTATAFDAAFAALARMRNQPEVRTVRQYADHPAYIHALAEQVRQYWAQHGRPDFAAGDKLVLSFHGVPKRTLDLGDPYHDQCQQTGALLMAALGLSTLECRVTFQSRFGKAEWLQPYTAPTLRELGAAGVRRADVFCPGFTADCLETIEEIGMEVRDEFIAGGGQAFHRIPCLNGAHAWIGALGEIVAENLQGWPVKAAQPEMVN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have metal ion binding, ferrochelatase activity within cytoplasm to regulate heme biosynthetic process.2. The protein design should consider the endogenous proteins and enzymes present in the protoheme biosynthesis, protoheme from protoporphyrin-IX: step 1/1 pathway to avoid interference with native metabolic processes.3. For general function, the protein need meet that Catalyzes the ferrous insertion into protoporphyrin IX.4. The designed protein should have a binding site that is specific to the target Fe cation ligand. The designed protein sequence is ",
    "response#1": "MVELISEQISLCESPSKAHIFFTAHGVPKSYVEEAGDPYKQQIEDCSLLIINELEKCLGYSNPHTLSYQSRVGPVEWLKPYTEEVLADLGRSNVNDLVVVPISFVGEHIETLQEIDIEYKEIAEKAGIKNFRRVKALE",
    "response#2": "QAMADLIAQELKKFPNPDQAHIFFSAHGVPQSYVDEAGDPYQAEIEACTRLIMRTLDRPNQYTLAYQSRVGPVEWLKPYTEEALQKLGAEGIDDLLVVPISFVSEHIETLQEIDIEYREIAEEAGIDNFQRVPALNRGDAFVEAMADVLAR",
    "response#3": "MVELISEQISLCESPSKAHIFFTAHGVPKSYVEEAGDPYKQQIEDCSLLIINELEKCLGYSNPHTLSYQSRVGPVEWLKPYTEEVLADLGRSNVNDLVVVPISFVGEHIETLQEIDIEYKEIAEKAGIKNFRRVKALS"
  },
  {
    "reference": "MDSDFVPPSVSFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFELPIPTKQRNVSSKPFHGYLCHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEAGRIAQSALHAFAAF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Probable 2-oxoglutarate-dependent dioxygenase that may be involved in glucosinolates biosynthesis; May play a role in the production of aliphatic glucosinolates .2. The stability of the protein-Fe(2+) complex should be optimized to maximize enzymatic activity.3. The protein should be able to bind 2-oxoglutarate, Fe cation ligand in a variety of conditions4. The protein contains novel Fe2OG dioxygenase domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MLIHVRDVLSAAELQQTRELLSDAPWADGGVTAGVQSAQVKNNQQLPQAHPVTQALQQLVMAGLNRHPTFFSAALPKKVFPPLFNRYGGTANAFGNHVDNAVRFITSGPHTGQRVRTDVSCTLFLAEPETYDGGELVIEDTYGSQRVKLAAGDLVLYPGTSVHRVEPVTRGARVGCFFWIK",
    "response#2": "MLIHVRDVLSAAELQQTRELLSDAPWADGGVTAGVQSAQVKNNQQLPQAHPVTQALQQLVMAGLNRHPTFFSAALPKKVFPPLFNRYGGTANAFGNHVDNAVRFITSGPHTGQRVRTDVSCTLFLAEPETYDGGELVIEDTYGSQRVKLAAGDLVLYPGTSVHRVEPVTRGARVGCFFWIR",
    "response#3": "MILQISNAVDTDTVKSIVAGLDAGQFSDGKKTAGWAAKDVKNNQQLSGKKSEAATQVLLDRLQQNALVQSVMRPKQVARVTINRYQQGEYYGTHMDDSLMNGVRTDISFTLGLSPLSDFEGGELVIEDASGERSWRLGQGDILMYPSHYLHRVNPVTKGSRLAMIGWVR"
  },
  {
    "reference": "MERLTFVTSNQHKFREVKAILGEYGIDVEHSPLSIVEIQSMAIEDIASAKAKNAYEILHRSVIVEDDALAINALNGFPGPYSSYVFKTIGNEGIIRLMHNIDDRRASFISVIAYCYSDGVEPVIFIGKVDGMIADGIRGKGWGYDPIFIPNCSSRTYAEMGDEKNRVSHRRLALERFAIWLKERQYSQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+) binding.2. The designed protein should have metal ion binding, ribonucleoside triphosphate phosphatase activity to facilitate purine nucleoside triphosphate catabolic process, nucleotide metabolic process.3. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..4. We are looking for a protein with broad substrate specificity for dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MRIIFATHNKHKYEEVRALVP",
    "response#2": "MCRFAL",
    "response#3": "MPIGVPKVPFRNPGEEDPSWVDVYRQLYRER"
  },
  {
    "reference": "MTENSNTDNILLTNLDDLINWGRANSLWPMFFGLSCCFVEMMTSFTSRYDVSRFGAEVLRGTPREADLMVIAGTVFRKMAPSILRLYEQMAEPKWVMAMGSCANSGGMYDVYSVVQGVNQILPVDVYIPGCPPRPEAFMQGLMLLQEKIRTSERPSRPVLHLHGGSQGTTGRVLVNGIDKSCDTRGSGMQGTSIRGAAAGHPSPWLSRSDEIWRPPAQKHDYEDFGLACELEAAFSDQVSLETNATDMLTYRVPQHLLQGVVRHLKSRANDPFRRLEDIAAVDNSCRRRREEFSDFTVNYHLLNFDRPGHIRLKVDLDGSAPSAPTITGIFPAASWYEREVFDMYGIGFTGHKNLRRILMPHDWQGHPLRKDHPFRATEMAPYTVDDARKHDALPAESFFPESTAETLILNLGPQHPGTHGIIRCILKLSGEEITDIDFDIGYHHRGAEKIAERQNWHQFIPYTDRIDYLAGVQNNLAYLNSVERLCSIKVPERAEVIRVMLSELFRIASHLVWLGTFTADIGAMTPVFYTFTDREKIFDIVELITGGRMHPAWFRIGGVADDLPDGWDKSVQELLNWLPPRLKEYHDLLDKNPILIARLKGVGAISKEDAFEWGLSGPNLRACGVEWDLRRKIPYCGYDRFDFDIPIAQHGDCLARYQVRMEEIQQSLKIVQQCLHNMPQGRWISEDCRFVLPKKRDSLNDIESLIHHFVNTTRGMSPPSGENYSAIEAPKGENGYFAVSDGLSTPYRLRIKTPSFPHIQALPVMTR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone.2. The designed protein must be optimized for NADH dehydrogenase (ubiquinone) activity, 4 iron, 4 sulfur cluster binding, NAD binding.3. The designed protein must have at least one NADH:ubiquinone oxidoreductase-like 20kDa subunit, NADH:ubiquinone oxidoreductase 30kDa subunit domain.4. The presence of [4Fe-4S] cluster is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "DVGAMTPVFFTFTDRQKAYTVIEAITGFRLHPAWYRIGGVAHDLPRGWEKLVKDFVEWLPKRLDEYTKAALQNSILKGRTIGVAQYNTKEALEWGVTGAGLRSTGCDFDLRKARPYSGYENFEFEVPLAANGDAYDRCMVRVEEMRQSVKIIDQCLRNMPEGPYKADHPLTTPPPKERTLQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGGTMSYRTRIRTPSFAHLQQIPSVIKGSMVADLIAYLGSIDFVMADVDF",
    "response#2": "DVGAMTPVFFTFTDRQKAYTVIEAITGFRLHPAWYRIGGVAHDLPRGWEKLVKDFVEWLPKRLDEYTKAALQNSILKGRTIGVAQYNTKEALEWGVTGAGLRSTGCDFDLRKARPYSGYENFEFEVPLAANGDAYDRCMVRVEEMRQSVKIIDQCLRNMPEGPYKADHPLTTPPPKERTLQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGGTMSYRTRIRTPSFAHLQQIPSVIKGSMVADLIAYLGSIDFVMADVDG",
    "response#3": "DVGAMTPVFYAFESREKIFDIVEMITGGRMHPAWFRIGGLPLDLPDGWKQVVDAFTVDFDKRIDEFNTLLTDGPIFRVRTEGIGVVTRDEAVEWGLSGPNLRATGMPWDLRRSMPYSGYDRYDFDVVTVDGGDCFARYLVRLKEMRESLKIIRQAAERMPGGRWMTDDYRFAYPKRSDGLNDIDSLIPHFVNVSRGPTAPKGESYRAIESSKGECGYYAVSDGGSVPYRMRIRTPSFPHLQALR"
  },
  {
    "reference": "MVKIRLTRLGRHKLPFFRIVVIDSRARRDGAYIEKVGTYEPFEGVVNINEEIALSWLKKGAQPSDTVKNLLREQGVWKKFMDSKVQSKKEHNANKVKKEVKKPEAKKAAASKPASKPSASKSASQKKTVSKK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that contributes to translation by functioning in ribosome, cytoplasm, ribonucleoprotein complex with structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "MPIGVPKVPFRLPG",
    "response#2": "GLYKTL",
    "response#3": "RRLQTIVYKKGLARTIYQARQLITHGHIAISGRKVTSPGYVVLRGEEDLIDYYPTSPFKQNPF"
  },
  {
    "reference": "MNNLSAQENTPSLPQSVWPADQMSLLERQAADAHGLSLYALMERAGEAVWQIASRRFPAAQHWLILCGPGNNGGDGYVIARLALMAGKQVTVLASEDQQALPEEAQQARQAWRAVGDVQSIDTAWPTTYDIVIDALLGNGLNRAPSVALAAAIEQANQSPAPVVAVDVPSGLSAANGAIPGACIQAALTVSLIALRPGLLTGKARDVVGTLQVATLGLHAWLEKRTAPWQRIDAAQLINWLPARRATSHKGDHGKLLLVGGAAGTAGAIRLAGEAALRSGAGLVRVLTHSSNVAPMLTARPELMVDELGRDRLEQALAWADIVVVGPGLGQEKEAQQALEQIENTQKPMLWDADALNLLAFRPQKRQNRVITPHPGEAARLLNLKVSDIESDRLHAAQLLARRYGGVVVLKGAGTLIVSEAGEQAIADVGNAGMASGGMGDVLSGIIGSLMAQGLALFTAASAGCVVHGAAADQLARHHGMRGMLASDLFSEIRQLVNPGITLKE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.2. The designed protein must be able to perform NADHX epimerase activity under specific conditions. The designed protein sequence is ",
    "response#1": "PTAHKGQLGHVLVVGGDHGTGGAALLSAHSALRSGAGRVSLATRGEHLAAAQSRLPEVMSLAVASANQLHGLGGNITVWVVGPGLGQSAWGRSLLSVAVTAPAAQVWDADALNLLAEGVVSIPPGSVVTPHPGEAARLLGISTRALQADRPAAARALAQRYQVICVLKGAGTLIADPTGCLLVCDRGDPVMAGAGLGDVLAGLIGALIAQGCTPWQAAGLGVWLHAGAGEYLAGKGNGLAASDLCDAIR",
    "response#2": "LLVVAGSEGMTGAAALAGEAAMRVGSGLVSVAVPQSLRTVIEMKLTECLKLGLEESEPGVLGEGAVRQLTPHLHRYHATLLGPGLSTRGEVARVVVALIERAEHPMVLDADALNILAGTLESLEVAKAPLILTPHPGEMARLLRTSTARVQENRLSIAHDFATRHGVVLVLKGARTLIATPEGEVFVNPTGNAGMASGGTGDVLAGMIGGFLVQGHPPRTAALLGVYLHGLAGDLAAEALGERSFIASDLLDRLAGDVLPHLPARAPTAHKGQLGHVLVVGGDHGTGGAALLSAHSALRSGAGRVSLATRGEHLAAAQSRLPEVMSLAVASANQLHGLGGNITVWVVGPGLGQSAWGRSLLSVAVTAPAAQVWDADALNLLAEGVVSIPPGSVVTPHPGEAARLLGISTRALQADRPAAARALAQRYQVICVLKGAGTLIADPTGCLLVCDRGDPVMAGAGLGDVLAGLIGALIAQGCTPWQAAGLGVWLHAGAGEYLAGKGNGLAASDLCDAIRHLLQERAR",
    "response#3": "PETHKGDYGHLLVIAGSVGKTGAAAMVCEAALRTGAGLVTLAIPKSLNAIMEVKLTEAMTEPLPETPKQTLGLRAFHSILHLCEDKKAVVIGPGIGTYKETQLLILKLIRTLNLPIVLDADGLTALATQMKLFPTANRSLILTPHPGEMARLLQTSAKEVQEHRIDVSRRFSQTHQVHLVLKGNRTLIATPKGEIFVNPTGNPGMASGGTGDVLTGMIGGLICQGFGILPSLQTAVFLHGWAGDEAVQECGEKSLVATDLIKKIK"
  },
  {
    "reference": "MGSSEGQETHVLMVTLPFQGHINPMLKLAKHLSLSSKNLHINLATIESARDLLSTVEKPRYPVDLVFFSDGLPKEDPKAPETLLKSLNKVGAMNLSKIIEEKRYSCIISSPFTPWVPAVAASHNISCAILWIQACGAYSVYYRYYMKTNSFPDLEDLNQTVELPALPLLEVRDLPSFMLPSGGAHFYNLMAEFADCLRYVKWVLVNSFYELESEIIESMADLKPVIPIGPLVSPFLLGDGEEETLDGKNLDFCKSDDCCMEWLDKQARSSVVYISFGSMLETLENQVETIAKALKNRGLPFLWVIRPKEKAQNVAVLQEMVKEGQGVVLEWSPQEKILSHEAISCFVTHCGWNSTMETVVAGVPVVAYPSWTDQPIDARLLVDVFGIGVRMRNDSVDGELKVEEVERCIEAVTEGPAAVDIRRRAAELKRVARLALAPGGSSTRNLDLFISDITIA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have a high affinity for UDP-alpha-D-glucose.2. For general function, the protein need meet that Possesses low quercetin 7-O-glucosyltransferase activity in vitro.3. The protein must have high specificity towards indole-3-acetate beta-glucosyltransferase activity, quercetin 7-O-glucosyltransferase activity, quercetin 3-O-glucosyltransferase activity, UDP-glucosyltransferase activity. The designed protein sequence is ",
    "response#1": "MEIILANPRGFCAGVDRAISIVERALELYGPPIYVRHEVVHNKFVVDGLKNRGAIFIEELSEVPDDVILIYSAHGVSQAVRNEARSRNLTMLDATCPLVTKVHMEVARASRKGKEAILIGHAGHPEVEGTMGQYSNPEGGMYLVESPADVWNLQVKDEDNLCYMTQTTLSVDDTSDVIDALKARFPKIVGPRKDDICYATQNRQDAVRDLASDADVVLVVGSKNSSNSNRLAELAQRVGKPAYLIDSAADIQEAWIKDATKVGVTAGASAPDILVQQVIQRLKQLGGSAVELIQGREENIVFEVPKELRVDVREVK",
    "response#2": "MERKNKYKHD",
    "response#3": "MELTFRILFLVLPIIAVLSLVLGPVLIRFSESVGKVRSIGPSPHQ"
  },
  {
    "reference": "MAPITFRKSYTIVPAEPTWSGRFPLAEWDQVGTITHIPTLYFYDKPSESFQGNVVEILKTSLSRVLVHFYPMAGRLRWLPRGRFELNCNAEGVEFIEAESEGKLSDFKDFSPTPEFENLMPQVNYKNPIETIPLFLAQVTKFKCGGISLSVNVSHAIVDGQSALHLISEWGRLARGEPLETVPFLDRKILWAGEPLPPFVSPPKFDHKEFDQPPFLIGETDNVEERKKKTIVVMLPLSTSQLQKLRSKANGSKHSDPAKGFTRYETVTGHVWRCACKARGHSPEQPTALGICIDTRSRMEPPLPRGYFGNATLDVVAASTSGELISNELGFAASLISKAIKNVTNEYVMIGIEYLKNQKDLKKFQDLHALGSTEGPFYGNPNLGVVSWLTLPMYGLDFGWGKEFYTGPGTHDFDGDSLILPDQNEDGSVILATCLQVAHMEAFKKHFYEDI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Required for the biosynthesis of these conjugated spermidine derivatives, specifically in anther tapetum..2. The designed protein must be optimized for spermidine:feruloyl CoA N-acyltransferase activity. The designed protein sequence is ",
    "response#1": "DVWFTERNESIALSLKHDGKLYDEDSEFQKVEVYKTFAYGNMLTLDGMVMTTEKDEYVYHEMISHVPMQSHPNPKRVLVIGGGDGGTARELLKYDDLEEVVMVEIDEKVVEASKEFLPTIASAFGHPKLNLKIEDGIKYVNEAPDESFDIVLVDSTDPVGPGEGLFTVEFYAQVHRILKKDGIMVTQSESPRFNEKVFVEIYDCYRGIFGKENVHCYLVHIPTYPTGMWSFSYSAG",
    "response#2": "SRGHRARQLTEPVGRGLSRSWEVSEVLFETRTEFQHLLIGRTAQGISLFCDGERQSTEATQLTYHEALMVPPMLLAARVRRVLIIGSSEGVASRLAVAEGAEIVDHVDIDQVAVRACAEHLPYGYTPAELAAAERGDGQVRVHYRDGWEFLAGAEPGYDVVVIDLPDENDDPSAQHNRLYGTEFLARCTALLAPGGVVSSQAGCPTMWRNETLISAWRRFTGAFGTVVYYGSDEHEWAFLSGRVAPA",
    "response#3": "SRGHRARQLTEPVGRGLSRSWEVSEVLFETRTEFQHLLIGRTAQGISLFCDGERQSTEATQLTYHEALMVPPMLLAARVRRVLIIGSSEGVASRLAVAEGAEIVDHVDIDQVAVRACAEHLPYGYTPAELAAAERGDGQVRVHYRDGWEFLAGAEPGYDVVVIDLPDENDDPSAQHNRLYGTEFLARCTALLAPGGVVSSQAGCPTMWRNETLISAWRRFTGAFGTVVYYGSDEHEWAFLSGRVAPLED"
  },
  {
    "reference": "MDDQNKNLLLATALSFLVILGWYFFFPPPEEAPQPATEVTETAPQGDTTAPAAAPSAGAATAEVDAAAAETEITEDVPRLTIDTPRVEGSISLKGGRIDDLRLKDYRETLDDDSPIVTLLSPAGQPHAYYALYGWAPGAGLGIEDVPTANTLWQAEAGATLTPDTPVTLTWDNGKGLTFSREVSIDEDYMFSITQSVTNSSGASVALAPYGTLARHGEPANLENFFVLHEGVVGMADGELSEIDYDDMTDFDPDPRDGSRAQVNTVTENGWIGFTGHYWMSTLIPAPGEAFRAIAKYDERRDIYQTDVVLPTVTLAAGESTSANTQLFAGAKEWATIREYERAGIEGFLDSIDWGWFFFFTKPIFAVLHWLNAAIGNMGVAIIALTFLLKILVFPLAYKSYASMARMKELQPEMEKLRERAGDDRQKMQKEMMELYKREKVNPAAGCLPILIQIPIFFSLYKVIFVTLELRHAAFFGPFQDLSVPDPTSLFNLFGLLPWAAPAPDSLLSLVFIGILPILLGVSMWVQQKLNPAPTDETQRMIFAWMPWVFMFMLGGFASGLVVYWITNNVITFTQQYLIMRSHGYTPDVFGNIKSGFKKDKAEKAEKK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be able to modulate protein transport in a way that leads to a desirable outcome.2. The protein must exhibit the following characteristics: Aids folding of multispanning membrane proteins.; Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. The designed protein sequence is ",
    "response#1": "DLIQIKTDVLDLAVDPQGGDIAQLRLPLYPRRQDHPEIPFQLFDNGNERTYLAQSGLIGSNGPDASPAGRPIYSAEKKSYQLADGQDQLVVDLKFSKDGVNYIKRFTLKRGLYDITVSYLIDNESAQPWTGAMFAQLKRDASSDPSSSTATGTATYLGAALWTSSEPYKKVSMKDMDKVNEDKSKAPITENVQGGWVAWLQHYFVTAWIPPKGENNQVLARKDSKGNYIIGYTGPSMTVAPGAKAETSAILYAGPKSQAVLKELSPGLELTVDE",
    "response#2": "PVINVTTDVLQLKLDGFSILAADLLRFPQSKDRGAKPIKLLTDDPNYPYSATTGWVSQSNSPVPNLSTFLPEQPDVSYKLANDQNRLVVPFIWTAANGVSIRRTFTFERGRYAILIRDEIRNSGETPWNAYVFRKLSRVPIPNILNRAMTNPDSFSFNGAVWYSEKGGYERRAFKDYMNDGGLNREIGGGWIALLQHHFFTAWIPQKDQASLYLLAQNGSRDIAELRGPAFTVAPGQTTTTEARLWVGPKLVEQITKEHVKGLDRVVDC",
    "response#3": "PRLPILTESVEGSVNLKGLRLDDLTLIRHRETLAKNSPAVRLFSPAGTENAWFTSFGWTGENVALPNADSLWKADSDKLTADHPVNFYWNNQQGQIFRITLSVDHDYMFNATESVINRGNSPVIVQPYVLTNHQGVFKTASSWTLHTGPIGVFNGSVNYHVDFADIDKATNNSIRNNTQGGWIGFSDKYWLTALAPHNQKIAIDTDFRSSSNHHYQADFTVAPVVVAAGKTASTSVDVFAGAKEVRVLDRYRDQLHLPHFDKAIDC"
  },
  {
    "reference": "MPTTQKTLMFLSGFLTSLGSVVVICSILGTQAWITSKIFFTDTISNGTIAITYGLFRGTSSQDLNEGLQELDKNFEVLGILSNSSQKSLHVVVIILLILSLAASLLSSMFTFYNSISNPYQTFLGPMGVYTWNGLSASFVFLTMVLFVGNVDSNHLSEKLSQTLYPDAINKKTTHTYGYSFWLILLVILLNIVTVVIIIFYQKARYHQKQEQRKPVEYAPRDGILF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The designed protein should have a specific function within the membrane. The designed protein sequence is ",
    "response#1": "GTLSILAAILMETKLHSPMYFFLGNLSLPDVGCVSVTVPAMLSHFISNDRSIPYKACLSELFFFHLLAGADCFLLTIMAYDRYLAICQSLTYSSRMSWGIQQALVGMSCVFSFTNALTQTVALSPLNFCGPNVINHFYCDLPQPFQLSCSSVHLNGQLLFVAAAFMGVAPLVLITVSYAHVAAAVLRIRSAEGRKKAFSTCSSHLTVVGIFYGTGVFSYTRLGSVESSDKDKGIGILNTVISPMLNPLIS",
    "response#2": "GTLSILAAILMETKLHSPMYFFLGNLSLPDVGCVSVTVPAMLSHFISNDRSIPYKACLSELFFFHLLAGADCFLLTIMAYDRYLAICQSLTYSSRMSWGIQQALVGMSCVFSFTNALTQTVALSPLNFCGPNVINHFYCDLPQPFQLSCSSVHLNGQLLFVAAAFMGVAPLVLITVSYAHVAAAVLRIRSAEGRKKAFSTCSSHLTVVGIFYGTGVFSYTRLGSVESSDKDKGIGILNTVISPMLNPLIS",
    "response#3": "GTLSILAAILMETKLHSPMYFFLGNLSLPDVGCVSVTVPAMLSHFISNDRSIPYKACLSELFFFHLLAGADCFLLTIMAYDRYLAICQSLTYSSRMSWGIQQALVGMSCVFSFTNALTQTVALSPLNFCGPNVINHFYCDLPQPFQLSCSSVHLNGQLLFVAAAFMGVAPLVLITVSYAHVAAAVLRIRSAEGRKKAFSTCSSHLTVVGIFYGTGVFSYTRLGSVESSDKDKGIGILNTVISPMLNPLINS"
  },
  {
    "reference": "ACQLITAEDSRGTQLHRALRSTSKVSKSTSCVEAGSYCRPNVKLCCGFCSPYSKICMNFPKN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be optimized for extracellular region-specific folding and stability, while maintaining toxin activity, calcium channel regulator activity.2. For general function, the protein need meet that Omega-conotoxins act at presynaptic membranes, they bind and block voltage-gated calcium channels (Cav).3. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "CLPDEYFCGFSMIGALLCCSGWCLGIL",
    "response#2": "SCISKL",
    "response#3": "ML"
  },
  {
    "reference": "MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSASANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFYNPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDSTPRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGTRASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYMRRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAATLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTDRFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTRVAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQLYSGPVRMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRVPFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The intended protein structure should align with strand-helix-strand-helix-helix-strand-strand-helix-strand-turn-strand-strand-helix-strand-turn criteria.2. The protein must exhibit the following characteristics: Receptor for the bacteriocin pesticin and for the siderophore yersiniabactin..3. The designed protein should contain at least one TonB box motif to achieve its intended function.4. The protein should be optimized for stability and solubility when expressed in cell outer membrane where it will be involved in siderophore transmembrane transport. The designed protein sequence is ",
    "response#1": "LPASGKVIYKGVWHFVTDTKKGQDFREIIQPSKKQGDRYSGFSGDGSEEYSNKNESTLKDDHEGYGFTSNLEVDFGNKKLTGKLIRNNASLNNNTNNDKHTTQYYSLDAQITGNRFNGTATATDKKENETKLHPFVSDSSSLSGGFFGPQGEELGFRFLSDDQKVAVVGSAQ",
    "response#2": "LPASGKVIYKGVWHFVTDTKKGQDFREIIQPSKKQGDRYSGFSGDGSEEYSNKNESTLKDDHEGYGFTSNLEVDFGNKKLTGKLIRNNASLNNNTNNDKHTTQYYSLDAQITGNRFNGTATATDKKENETKLHPFVSDSSSLSGGFFGPQGEELGFRFLSDDQKVAVVGSAQ",
    "response#3": "TTVLDAVELTLNDKKIKNLDNFSNAAQLVVDGIMIPLLPKDSESGNTQADKGKNGGTEFTRKFEHTPESDKKDAQAGTQTNGAQTASNTAGDTNGKTKTYEVEVCCSNLNYLKYGMLTRKNSKSAMQAGGNSSQADAKTEQVEQSMFLQGERC"
  },
  {
    "reference": "MNKILRGVIQFRNTIRKDLVKQFEEIKNNPSPTAVMFTCMDSRMLPTRFTQSQVGDMFVVRNAGNMIPDAPNYGAFSEVSVNTEPAALELAVKRGGIRHIVVCGHSDCKAINTLYGLHQCPKNFDVTSPMDHWVRRNGFASVKRLNERLHRGPSSMKFESEVAPSQSFDAIIDPMDTLAMEDKLSQINVLQQLINICSHEFLKEYLESGRLHIHGMWFDIYKGEDYLFSKDKKRFVVIDEKTVTDLLAELNARYPVPEDQDGPVAFAKSN",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must be able to carbonate dehydratase activity.2. The presence of Zn(2+) is crucial for the protein to function optimally under specific conditions.3. The protein must exhibit the following characteristics: Reversible hydration of carbon dioxide..4. The protein should be able to bind Zn(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "GTWCYDSQDPKCGPAHWKELAPACGGPTQSPINIDLRLVQRDYTLKPFIFQGYDSAPQDPWVLENDGHTVLLRVNSCQQNCPAIRGAGLPSPEYRLLQLHFHWGSPGHQGSEHSLDEKHGSMEMHMVHMNTKYQSMEDARSQPDGFAILAVLLVEEDRDNTNFSAIVSGLKNLSSPGVAVNLTSTFALASLLPSALRLLRYYRYSGSLTTPGCEPAVLWTVFENTVPIGHAQVVQFQAVLQTGPPGLHPRPLTSNFRPQQPLGGRRISASC",
    "response#2": "GPERWGELKPEWEMCGKGEMQSPIDLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQLHWHSPSEHTINGRRFALELHMVHEGRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAEMEEAEKNVGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVAVHDDANSNARPVQPTNKRIVR",
    "response#3": "CGPAHWKELAPACGGPTQSPINIDLRLVQRDYTLKPFIFQGYDSAPQDPWVLENDGHTVLLRVNSCQQNCPAIRGAGLPSPEYRLLQLHFHWGSPGHQGSEHSLDEKHGSMEMHMVHMNTKYQSMEDARSQPDGFAILAVLLVEEDRDNTNFSAIVSGLKNLSSPGVAVNLTSTFALASLLPSALRLLRYYRYSGSLTTPGCEPAVLWTVFENTVPIGHAQVVQFQAVLQTGPPGLHPRPLTSNFRPQQPLGGRRISAC"
  },
  {
    "reference": "MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVLMAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQGKAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDKK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Incorporation of the H-T-H motif domain in the designed protein should not interfere with its overall stability and folding.2. The protein must exhibit the following characteristics: Activates transcription of virulence factors alpha- and beta hemolysin genes (hla and hlb).3. The designed protein should contain one or more HTH marR-type domains that are essential for its function.4. The protein can be expressed in cytoplasm and perform DNA binding without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "HMGSLRVACYTAPALSFMSGVIQTFIADRPDVSVYLDTVPSQTVLELVSLQHYDLGISILAGDYPGLTTEPVPSFRAVCLLPPGHRLEDKETVHATDLEGESLICLSPVSLLRMQTDAALDSCGVHCNRRIESSLALNLCDLVSRGMGVGIVDPFTADYYSANPVIQRSFDPVVPYHFAIVLPTDSPPPRLVSEFRAALLDAL",
    "response#2": "HMGSLRVACYTAPALSFMSGVIQTFIADRPDVSVYLDTVPSQTVLELVSLQHYDLGISILAGDYPGLTTEPVPSFRAVCLLPPGHRLEDKETVHATDLEGESLICLSPVSLLRMQTDAALDSCGVHCNRRIESSLALNLCDLVSRGMGVGIVDPFTADYYSANPVIQRSFDPVVPYHFAIVLPTDSPPPRLVSEFRAALLDAL",
    "response#3": "HMGSLRVACYTAPALSFMSGVIQTFIADRPDVSVYLDTVPSQTVLELVSLQHYDLGISILAGDYPGLTTEPVPSFRAVCLLPPGHRLEDKETVHATDLEGESLICLSPVSLLRMQTDAALDSCGVHCNRRIESSLALNLCDLVSRGMGVGIVDPFTADYYSANPVIQRSFDPVVPYHFAIVLPTDSPPPRLVSEFRAALLDAL"
  },
  {
    "reference": "MTTEIHSNPANRPNRIASVERNTAETQVSVTVNLDGTGQGKIDTGVPFLDHMLDQIKRHGLFDLDITCKGDTYIDDHHSIEDTGITLGQAFAKALGDKKGIRRYGHFYAPLDESLTRAVVDISGRPGLHMDIPFTRSHVGNFDVDLFSEFFFGFVNHAWMTVHLDNLKGKNSHHQIECTFKAFARALRMACEYDERALNTLPSTKEAL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that is capable of catalyzing reactions within the L-histidine biosynthesis, L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9 pathway with high efficiency and specificity.2. The protein design should aim to mimic or enhance the function of natural proteins involved in histidine biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.3. The protein should be designed to have high stability and activity towards D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-   4-yl)-2-oxopropyl phosphate + H2O. The designed protein sequence is ",
    "response#1": "MDTKKLIVLGNNTHHKIEALFGHKTLKEVDKLLKDYATEQGLDMNYFTSNHEGEIVDRIHEAYDEGVDFIIINPAAFTHTSVALRDALLGVDIPFVEVHLSNVHAREPFRHHSYLSDKAVGVICGLGPDGYEFALNAAVNRLKG",
    "response#2": "MDAPTAPDPQA",
    "response#3": "MEKKLLIATRNKGKTKEIKKILKDLDFDVLSLLDFPNPPDFVEDGLTFTENALKKARYVAKLTGKPALADDSGLCVDVLGGAPGIFSARYAGEGANDAKNNEKLLLEMKNVPAGKRTAHFECVIALATPDGTLHTFTGICSGTITENIRGTKGFGYDPLFIPEGFEVTFAEMEPEEKNRISHRGKAFRNLKNLLEK"
  },
  {
    "reference": "EAGEECDCGTPENPCCDAAT",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein contains novel Disintegrin domains that confer a unique function or activity.2. For general function, the protein need meet that Inhibits fibrinogen interaction with platelets3. The protein must have high specificity towards toxin activity. The designed protein sequence is ",
    "response#1": "PDSGGCKHASEELGVLCPTGCELRTELLKQEKTVKPTIQDLTDKVKKLEQSSTTIYAFSNILDERLAKRQKQIRDNQNVISEYNREVEEHYSFIKENLDNNIPSNLKVLRGIVDILHQKLVKLDNAITSQLGECRTPCTVSCNIPC",
    "response#2": "MNSANPCCDPITCKPRRGEHCVSGPCCRNCKFLNPGTICKRTMLDGLNDYCTGVTSDCPRNPWKSC",
    "response#3": "VIGGDECNINEHRFLVALYDGLSGTFLCGGTLINQEWVLTAQHCNRSLMNIYLGMHNKNVKFDDEQRRYPKKKYFFRCNKNFTKWDEDIRLNRPVRFSAHIEPLSLPSNPPSEDSVCRVMGWGQITSPPETLPDVPHCANINLFNYTVCRGAYPRMPTKVLCAGVLEGGIDTCNRDSGGPLICNGQFQGIVFWGPDPCAQPDKPGVYTKVFDYLDWITANM"
  },
  {
    "reference": "MKVCKVEEMRDLDRRATIDYGISQEILMENAGIASYDVIQKELGVKNKKFLVFCGGGNNGGDGFVVARKIRSSGGEIKVYLLTDREKYRDPAKKNLEIISKFSIEIDKAKINSIKDDISNTDAIIDAIFGIGLDRDVEGIYREVIQLINESKKPVFCIDIPSGINGNTGQEMGISVQANYTITFGMPKIGNLLYPGYGKGGKLYLTHISYPYQLQNSGEIKVELPELIPLPKREANTTKFSYGPVLTIAGAKNYYWAPYASAYSVLKAGGGYSFLACPKSLTPYIAVGGREIVFLPQEKETSRKSLSLEAKDNLLDEVEGRVPKMVIIGPGLSIDEETQNLIRELAKEIGKPLLIDGDGITAIAKDPEIVKERKAPTILTPHTGEMARIIKGLRKTEIKSVDIERDRVGFLQQACQELKAIIVLKGSHTLIGYPDQRVLINMSGDTDGQAGMATAGSGDVLNGTIAAMFCQGLDIEEAVQTGVFIHGLSGDLAAKEIGSRGVTASDILNFLPKAVKYYERNFDQISEDYYGKCYLI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must be optimized for NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity.2. The designed protein should be able to compete with endogenous proteins or inhibitors for AMP binding.3. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "MGDIESRIFAAGMPVAALMEKVGGQLAQRIQALYPNARTKRIGCLVGPGHNGGDALVVARELHSQGYDVTIYRPFGKLKDLTAQHAQYAERLGIPHCEQVEPLADCDVLVDGLFGFGLTRSLDGTIATIVDQINQWSTPVISIDVPSGLHTDSGAALGTAIRATRTLCLGLWKLAFMQDQALEYTGASELIDFGIPLADII",
    "response#2": "ERDAHKYARGLLAVIGGAMPGAGVLASRAAMHGGAGYVKLYSDHDGLPGVPPDLVVAGDPMPALATDPRIAAVLIGPGLGRSEEARSVLSWVLACDLPTVCDADALVLLEPEMLEDRSAPLVLTPHAGELDAIGNAFASSEPDRLEQVTELAEAIEGVLVAKGPDTIVAAPGRPPVFMPPAPSWLSTAGTGDVLAGLIASQLAVGETSAFQAAQNGCWLHREAALRAGPAFSASGLVF",
    "response#3": "VLVIAGSRGMSGAAVLTGHAAYKMGAGLVAFAPPQGIAPVLNAALLEAIVWPQPETEAGTLFWDARSSLLELAGQYDVVALGPGLSTNPQTVEFVEKLVADIEKPLVIDADALNALAGDAGILKSREALTVITPHPGELARLYGVSSKDVLADWLGFARRAQERFGAVVVLKGASTVICSAEETTINVTGNPGLATAGTGDVLTGFIAALIAQGVDAYNAACLGVYLHGFSADIAAEDIGELSLMASDVIEYLR"
  },
  {
    "reference": "MNKPQSFFHLHLISDATGETLLAAGRAASAQYKDARAIEHIYPLIRTEKQVAKVFEDIEEEPGIILYTVVDQKLARGIDERCATMGLPCVSVLEPVLTVFQSYLGTPAGRRVGAQHVLDAEYFRRIDALNFTMDHDDGQLPANMDDADVVLIGISRTSKTPTSIYLANRGIKTANIPIVLGVPVPESLVSASKPLIVGLIATAERISHVRQNRILGNSSSYVPTDYVDRAAINEELAYARQICTRHGWPMIDVSRRSIEETAAAIVALRGKNR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein with protein serine/threonine kinase activity, ATP binding, ADP binding functions is required to enable protein dephosphorylation.2. The protein should be designed to catalyze the following reaction H(+) + N(tele)-phospho-L-histidyl/O-phospho-L-threonyl-   [pyruvate, phosphate dikinase] + phosphate = diphosphate + N(tele)-   phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase].3. The designed protein should be able to compete with endogenous proteins or inhibitors for ADP binding.4. The protein must exhibit the following characteristics: Bifunctional serine/threonine kinase and phosphorylase involved in the regulation of the pyruvate, phosphate dikinase (PPDK) by catalyzing its phosphorylation/dephosphorylation.. The designed protein sequence is ",
    "response#1": "MALLEPGAWLDTTDAGRLGRPFVPLDPADYAAIVRGEPVDPERADWPEDAVLLERDQYFAKHRLKAEAVRAAEGLPVFVFPTPTA",
    "response#2": "MVPDPSAHRPEPPAVPPRRRLTGLVAAAAALSATGAGALAQDSWTGEHGWRSIFGLGAVLCLAGGLVVLVRPRGRAPAVAAPPSAAPPTGVAAAPAAAPTPAPSAPPGPEASPAPAPGVAAALAPLDPTAPARYRAPGAVAPGEPRTPPPEQPPVLADGA",
    "response#3": "MNNNNIAIILAAGSSTRMGFDKKQFLKINNIPILIHTLNVFKKCNLIDNIIVVTKKDYEETTKKILEKFKLNNIKNIINGGNTRQQSVFLGLKTIEKKCKYVAIHDGARPFLSKKVINNAINNNNYKGAIPVLNIKDTIKVVNKKGYIEKTIQRENLWKAQTPQIFKFNSIKKCHKKSLNENKNVTDDVSIVEFLGFKVKIIEGNIKNIKITTKNDLLYAENILKNNI"
  },
  {
    "reference": "MDTNVQKRRENKKSLRVKVISLGNAEVGKSCIIKRYCEKRFVPKYLATIGIDYGVTKVQVRDREIKVNIFDMAGHPFFYEVRNEFYKDSQGVILVYDVGLRESFDALDSWLTEMKQEMGSQANMENIIFVVCANKVDLTKRRVVDESEGRLWAESRGFHYFETSAQSGEGINEMFQSFFSSITDMCENGGKRPTPEVSVGFTKEQADTIRRIRNSKDSWDMLGVKPGATREEVNKAYRKLAVLLHPDKCVAPGSEDAFKAVVNARTSLLKNIK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be optimized for GTPase activity. The designed protein sequence is ",
    "response#1": "FKADLLWMDDEELIVGRSFLIKIGTKVTQATIIKIHYCIDINTGSHILQKKAEKNDLINCDISLAEKVVLDKFKVHKDLGRFILINRITHATSACGVIL",
    "response#2": "FKADLLWMDDEELIVGRSFLIKIGTKVTQATIIKIHYCIDINTGSHILQKKAEKNDLINCDISLAEKVVLDKFKVHKDLGRFILINRITHATSACGVIL",
    "response#3": "FKADLLWMDDEELIVGRSFLIKIGTKVTQATIIKIHYCIDINTGSHILQKKAEKNDLINCDISLAEKVVLDKFKVHKDLGRFILINRITHATSACGVIV"
  },
  {
    "reference": "MNGEEEFFDAVTGFDSDNSSIGEFSEANKISGMIDLDTSKSTRSGKNGEKPQQENGIQKHRTALPAPMFTRSDFSVWSILKKCIGLELSKITMPIAFNEPLSFLQRITEYMEHVYLIHKASSQSQPLERMQSVAAFAVSAVASQWERTGKPFNPLLGETYELIREDLGFRFISEQVSHHPPISAFYSEGLNQDFRFHGSIYPKLKFWGKSVEAEPRGTITLELLKHNEAYTWTNPTCCVHNVILGQLWIEQYGIVEIVNHRTGDKCILHFKPCGLFGKELHRVEGYIQDKNRKKLFIMYGKWTECLWGIDPASYESFKKQEKRGDQARKAKMDDGPEKANSDVPGDVADDVPVAQETVQVIPGSKLLWRINSRPPNSAQMYNFTSFTVSLNELESGMEKTLPPTDCRLRPDIRGMENGNMDLASQEKERLEEKQREARKERAKEDAEWRTRWFSPGNNPYTGAPDWLYAGHYFERNFSDCPDIY",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The ideal protein would be one that plays a critical role in phospholipid transport, intracellular cholesterol transport, protein homotetramerization through cholesterol transfer activity, sterol transporter activity, cholesterol binding within membrane, cytosol, lipid droplet, intracellular membrane-bounded organelle, extrinsic component of cytoplasmic side of plasma membrane.2. The protein must exhibit the following characteristics: Exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate; Increases plasma membrane cholesterol levels and decreases phosphatidylinositol-4,5-bisphosphate levels in the cell membrane; Intracellular transport protein that binds sterols and phospholipids and mediates lipid transport between intracellular compartments; Binds phosphoinositides, such as phosphatidylinositol-4,5- bisphosphate.3. The protein should exhibit Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "LNQETAQVIQAAFRNANFPNITGERSVRLLGKVAYGLSNIQVNDLSIEQSEVELKENDAIDIAIKNVTAVFKGTLTYGYAGAWFLQLFHSVDFEIESSIDLQINIKLMCQEDQVAADASDCYLSFHKLTLHLQGDKEPGWLKQLFTDFISFTLKFVLKREVCNEINYGIIRIHLLAARGLSSKDKYVKGLIEGKSDPYALVRLGTQTFCSRVIDEELNPQWGETYEVMVHEVPGQEIEVEVFDKDPDKDDFLGRMKLDVGKVLQASVLDDWFPLQGGQGQVHLRLEDVEKAEWLNKIVAQVWPFLGQYMEKLLAETVAPAVRGSNPHLQTFTFTRVELGEKPLRIIGVKVHPGQRKEQILLDLNISYVGDVQIDVEVKKYFCKAGVKGMQLHGVLRVILEPLIGDLPFVGAVSMFFIRRPTLDINWTGMTNLLDIPGLSSLSDTMIMDSIAAFLVLPNRLLVPLSESRMLYFWLSEHILNSLASAAFLDERLVMNISGKKLQALFEIEDTEVQQKAVQLIFQGTSYNDSVAKVWSLTHPEISLQPEGTVVKSLVAVEINIFPPGEESLMVLYMEKEITVTIQAAYAEKKLILQPLDSRIEFKVFKWAADPSENDQSIRNFLQKMISVVGIPEVISRIEPALTSLMNSKGLHLFEIRNPEIITRKGYLIVQLE",
    "response#2": "RMTKPAALLLNQETAQVIQAAFRNANFPNITGERSVRLLGKVAYGLSNIQVNDLSIEQSEVELKENDAIDIAIKNVTAVFKGTLTYGYAGAWFLQLFHSVDFEIESSIDLQINIKLMCQEDQVAADASDCYLSFHKLTLHLQGDKEPGWLKQLFTDFISFTLKFVLKREVCNEINYLAQVLANYVHDLAANFVRDEDIGLDISLASDPLIKANYLESHHKGLVMYKNVIRVHLLEAKKLAQKDNFLGLGGKSDPYAKVSIGLQHCRSRTIYKNLNPTWNEVFEFMVYEVPGQDLEVDLYDEDTDKDDFLGSLQICLGDVMKNRVVDEDVEKAEWLNKIVAQVWPFLGQYMEKLLAETVAPAVRGSNPHLQTFTFTRVELGEKPLRIIGVKVHPGQRKEQILLDLNISYVGDVQIDVEVKKYFCKAGVKGMQLHGVLRVILEPLIGDLPFVGAVSMFFIRRPTLDINWTGMTNLLDIPGLSSLSDTMIMDSIAAFLVLPNRLLVPLLNYKPEIFFAFGSPIGMFLTVRGLRRIDPNYKFPTCKGFFNIYHPFDPVAYRIEPMVAPGIEFEPMLIPHHKGRKRMHLELREGLTRMSMDLKNNLLGSLRMAWKSFTRGPYPALQASETAEETEAEPESSSEKSNEANTEEPPVEVKEEAPISVGMLNGGQRIDYVLQEKPIESFNEYLFALQSHLCYWESEDTVLLVLKEIKGAKEPPLVPDLQDVAQLRSPLPRGIIRIHLLAARGLSSKDKYVKGLIEGKSDPYALVRLGTQTFCSRVIDEELNPQWGETYEVMVHEVPGQEIEVEVFDKDPDKDDFLGRMKLDVGKVLQASVLDDW",
    "response#3": "RMTKPAALLLNQETAQVIQAAFRNANFPNITGERSVRLLGKVAYGLSNIQVNDLSIEQSEVELKENDAIDIAIKNVTAVFKGTLTYGYAGAWFLQLFHSVDFEIESSIDLQINIKLMCQEDQVAADASDCYLSFHKLTLHLQGDKEPGWLKQLFTDFISFTLKFVLKREVCNEINYLAQVLANYVHDLAANFVRDEDIGLDISLASDPLIKANYLESHHKGLVMYKNHWFYCKVLDSKELWIPFNSEDSQQLEDAYGSGKDCNERIVPTDGGRYDVHLGERMRYAVYWDELPSEVRKLSESRMLYFWLSEHILNSLASAAFLDERLVMNISGKKLQALFEIEDTEVQQKAVQLIFQGTSYNDSVAKVWSLTHPEISLQPEGTVVKSLVAVEINIFPPGEESLMVLYMEKEITVTIQAAYAEKKLILQPLDSRIEFKVFKWAADPSENDQSIRNFLQKMISVVGIPEVISRIEPALTSLMNSKGLHLFEIRNPEIITRKGYLIVQD"
  },
  {
    "reference": "MKRYNVAVVGATGAVGEEIFKILEERNFPVENLLPLASARSVGAEVQFAGKNYKVVELTEEVFEKHHVDIAFFSAGGSVSAKFAPYAAQAGAVVIDNTSHFRMDEDVPLVVPEVNPEDIALWRKRGIIANPNCSTIQMVQVLKPLHDAFEIERVDVSTYQATSGAGKKGMEELVYQMQKFFDFTLSECEPEVFAHQIALNLIPHIDVFQENGYTKEEMKMVKETNKIMHSHFAVSATCVRVPVLRSHSESITIRFKNSADAKEAREILSKAPSVVVMDEPLAKKYPMPSIATDTDETYVGRIRNDIYDEKILHLWCVADQIRVGAATNAVRIAQKWVEIEGEK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The phosphate binding site should be stable and able to withstand changes in environmental conditions.2. The designed protein should interact with enzymes and substrates within the L-threonine biosynthesis, L-threonine from L-aspartate: step 2/5, Amino-acid biosynthesis pathway to enhance or regulate its activity.3. A protein with NAD binding is needed to enhance isoleucine biosynthetic process, threonine biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "LNPIQEAVGIKRISVATYQAVSGSGKEAVKELAKQTAELLNGREAEYRVYPRQIAFNAIPHIDQFMENGYTKEEMKMNWETRKIFADDSIELSATCVRVPVFFGHSEAVQIETYKPISEKEVREILSEAAGITVIDEREDGGYPTAVHESAGQDSVFVGRIRQDISLPNGLNLWVVSDNVRY",
    "response#2": "LNPIQEAVGIKRISVATYQAVSGSGKEAVKELAKQTAELLNGREAEYRVYPRQIAFNAIPHIDQFMENGYTKEEMKMNWETRKIFADDSIELSATCVRVPVFFGHSEAVQIETYKPISEKEVREILSEAAGITVIDEREDGGYPTAVHESAGQDSVFVGRIRQDISLPNGLNLWVVSDNVRR",
    "response#3": "GLFVNNLIDWVFVSTYQAASGAGSRYVIELLKQMGSLYNVVSKDLLDKSYSVLDIEKKVTQESRSKNFPLENFSVPLATSLIPWIDVEMKNGQSREEWKGQAETNKILNLKKKVLIDGTCVRISSIRCHSQSFLIKLNKDISLENIKKIIVNHNQWVDVIPNNMQKTLCNLTPSAVTDTLNIPIGRLRKLNIDDRYLSAFTVGDQLLF"
  },
  {
    "reference": "MKGKKTLKRTIAAEESNGTSDVKKTKALPIVTHPSHGTVGGQVLTLGQGDVGQLGLGEDIMERKKPALVTLTEDIVQAAAGGMHTVCLGASGSIYTFGCNDEGALGRDTSEEGSEMQPGKVELAEKVVQVSAGDSHTAALTEDGRVFVFGSFRDNNGVIGLLEPMKKSMVPVQVQINTPVIKIASGNDHLVLLTVDGDLYTSGCGEQGQLGRVPERFTNRGGRKGLERLLVPQCIHLKAKGSGRVHFQDVFCGAYFTFAVSQEGHVYGFGLSNYHQLGTKNTQACYAPQNLTSFKNSTKSWIGFSGGQHHTVCVDSEGKAYSLGRAEYGRLGLGENAEEQSEPTPIPDLPKINSVASGASVSYAVSTDGCVFAWGMGTNLQLGTGEEEDVWSPEQMTGKHLEDREVLSVSSGGQHTVLLVRKRS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Binds both to the nucleosomes and double-stranded DNA .; Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis; Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import ; Involved in the regulation of onset of chromosome condensation in the S phase2. The protein should be optimized for stability and solubility when expressed in nucleus where it will be involved in cell cycle. The designed protein sequence is ",
    "response#1": "ENKEKSKEIPCLSGSAPASPPKLPVDASTFLSFPSPEKLVRMGPRRSAMIAQQVNVADTEKVVQAFIQVSSVYREDGEIKAAVEETIDGLMKEAFENRGFQANVFITSLLVQMGLLKSEDKMKTIPHLNGPLLTLNHMVQQNYFPKSLASTLLAFISKPNGVLENNASARHTLLCNLI",
    "response#2": "ENKEKSKEIPCLSGSAPASPPKLPVDASTFLSFPSPEKLVRMGPRRSAMIAQQVNVADTEKVVQAFIQVSSVYREDGEIKAAVEETIDGLMKEAFENRGFQANVFITSLLVQMGLLKSEDKMKTIPHLNGPLLTLNHMVQQNYFPKSLASTLLAFISKPNGVLENNASARHTLLCNLI",
    "response#3": "ENKEKSKEIPCLSGSAPASPPKLPVDASTFLSFPSPEKLVRMGPRRSAMIAQQVNVADTEKVVQAFIQVSSVYREDGEIKAAVEETIDGLMKEAFENRGFQANVFITSLLVQMGLLKSEDKMKTIPHLNGPLLTLNHMVQQNYFPKSLASTLLAFISKPNGVLENNASARHTLLCNLL"
  },
  {
    "reference": "MQSMKDAAASAKAGMEKAKASMQEKVDQMKTRDPNEKEMARERKEERQEDAELRKQEARHHNATAGHVGGGGIGGTGYTTAGYNIGDTGGYGGTGGHDNRGYPTAGSGYDTGRQDDLSSMGFGGDTEGAYGTTGNQDFPNAASNNAGTRRNTRGGTQDDPYYRSY",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be able to modulate response to abscisic acid in a way that leads to a desirable outcome.2. For general function, the protein need meet that LEA proteins are late embryonic proteins abundant in higher plant seed embryos3. The protein should exhibit Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "LERTTSLPAEMEEEWRKRKEMQSLRRMEAKRRRCEKC",
    "response#2": "LERTTSLPAEMEEEWRKRKEMQSLRRMEAKRRRCEKN",
    "response#3": "LERTTSLPAEMEEEWRKRKEMQSLRRMEAKRRRCEKI"
  },
  {
    "reference": "MPLVRYRKVVILGYRCVGKTSLAHQFVEGEFSEGYDPTVENTYSKIVTLGKDEFHLHLVDTAGQDEYSILPYSFIIGVHGYVLVYSVTSLHSFQVIESLYQKLHEGHGKTRVPVVLVGNKADLSPEREVQAVEGKKLAESWGATFMESSARENQLTQGIFTKVIQEIARVENSYGQERRCHLM",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a binding site that is specific to the target GTP ligand.2. The protein design should aim to mimic or enhance the function of natural proteins involved in positive regulation of NF-kappaB transcription factor activity, potentially with improved properties such as stability, specificity or selectivity.3. The engineered protein is expected to adopt a strand-helix-strand-strand-helix-turn-strand-helix-strand-helix-helix-strand-helix-based conformation.4. The protein design should include a Effector region motif to ensure proper function. The designed protein sequence is ",
    "response#1": "MTHQPRVGRGSFGEVHRMKDKQTGFQCAVKKVRLEVFRVEELVACAGLSSPRIVPLYGAVREGPWVNIFMELLEGGSLGQLIKQMGCLPEDRALYYLGQALEGLEYLHTRRILHGDVKADNVLLSSDGSRAALCDFGHALCLQPDGLGKSLLTGDYIPGTETHMAPEVVMGKPCDAKVDIWSSCCMMLHMLNGCHPWTQYFRGPLCLKIASEPPPIREIPPSCAPLTAQAIQEGLRKEPVHRASAMELRRKVGKAE",
    "response#2": "MTHQPRVGRGSFGEVHRMKDKQTGFQCAVKKVRLEVFRVEELVACAGLSSPRIVPLYGAVREGPWVNIFMELLEGGSLGQLIKQMGCLPEDRALYYLGQALEGLEYLHTRRILHGDVKADNVLLSSDGSRAALCDFGHALCLQPDGLGKSLLTGDYIPGTETHMAPEVVMGKPCDAKVDIWSSCCMMLHMLNGCHPWTQYFRGPLCLKIASEPPPIREIPPSCAPLTAQAIQEGLRKEPVHRASAMELRRKVGKAG",
    "response#3": "GPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVEKAIAHYEQQMGQKVQLPTETLQELLDLHRDSEREAIEVFMKNSFKDVDQMFQRKLGAQLEARRDDFCKQNSKASSDCCMALLQDIFGPLEEDVKQGTFSKPGGYRLFTQKLQELKNKYYQVPRKGIQAKEVLKKYLESKEDVADALLQTDQSLSEKEKAIEVERIKAESAEAAKKMLEEIQKKNEEMMEQKEKSYQEHVKQLTEKMERDRAQLMAEQEKTLALKLQEQERLLKEGFENESKRLQKDIWDIA"
  },
  {
    "reference": "MFSSKVNFHDSQLHFTCAIIILCPLIWNILARLCHKLLPEYTPKSVRYLTCYLLTIWIFSFSSYRDYLIRKTIQQQPKFIVPNEYSALVEFFAIGIFLLGQLFVLSSFYKLGVTGTFLGDYCGILMDAPVTSFPFNVLNNPMYIGSTLSFFAMALYYASSVGILLTIEVYIVYRIALQFEEPYTKWIYSQKKKMK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be able to bind S-adenosyl-L-methionine ligand in a variety of conditions2. A protein that contributes to methylation by functioning in mitochondrial membrane, endoplasmic reticulum membrane with phosphatidyl-N-dimethylethanolamine N-methyltransferase activity.3. The protein have enhanced a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + S-adenosyl-L-   methionine = 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine +   H(+) + S-adenosyl-L-homocysteine.4. The protein must exhibit the following characteristics: Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure.5. The Cytoplasmic, Lumenal topological domain of the protein should be designed to be flexible or rigid.6. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "MANINRPFNRDDYGNQLDPNKLRQDFNFDENLISDQGYYDYRPDQDMFDFELDPLAGSPISPINYHQSYYPPQQQQQQQQQQQQPQSQAQQQTQEQQQAQQHAQAQQHAQAQQQAQQQAQQQQAQQQAQQQQQQQQQQPTIHTTQPTQTQPQQPNQPQQPQPNQPQQPQPTQPTQQPAQNQPSQPQTPQPQQPQQPQQPQQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQSTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQSTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQPTQ",
    "response#2": "MSTAALRDKQANRADGLVNGHANGHTNGHAATNGHENGSSNGHGNGLANGHANGHANGQANGHAATNGHSNGLNGHAATNGHAHENGEPSENGRAANGHSNGHAANGHSNGHAANGHGNGANGHANGHATNGTNGHANGHANGSANGNGHAHANGHANGNGHANGNGHAANGHGNGSDPKEAKKEPALDPAKALEEARKATAATWARHIGGGFSPDLDPTDPAAFWNRALPEEYMADLKRRLGDGPFAGTKTPPFSFHMPFNTWAPEAEHKHLNFYLGADPASRPWQDPDGKWAGYGDPKTQWGFYCSLDHPAPGEEHPRPYFHQLYVFHHPTKENPYSLVALTSPANHEPGDHVMPCILSGLDLKSNEGRFVAFNGNSGQLNHPANGQPIHYFHTNWEATHPDYPTLLIFAEENVTPQTLNWVHQETSEKFAYRINWNKPRAAQK",
    "response#3": "MAYSFPRGFKCPICSKSVSSDEMDLHLVMCLTKPRVTYNEDVMTKDAGFSSDDCYGGYKQAGVILSSKQGESVISIYRSDKPTNNSPSNSEKAEAIVASTGVRLPRRKEEQEQASTAKSSGSTVSASQSSADDLSTPPKSSSGSKASGSNKEGPRQNQNKARSWEGAMSVGSSSVPVTSSSTSTSPVTTSSSSVSTSRISTPTPSTSANSGLSCNVCQKKFNSLQAFGIHAGSTGHAAFKGYR"
  },
  {
    "reference": "MNVATVILAAGQGTRMRSKLPKILHPLRGQPMIDYTVNLAMQLSSQPPVLVVGHGAKLVHNQVGECVRYVLQETRLGTGHALLQAQELLQGRSEAVFVLYGDMPLLTPPTCSRVIESFSQATPTPAIAMLTLTIPEGASSMGFGRVERDAQGRVTGVVEEFQATPEQLRLRELNCGIYCFRADWLWERLPKLPISSKGEYLLTDMVEVAAREGEPIIAVPVDDPDDVLGINDRILLSQAEKILQRRINIELMRGGVTLIDPDTTYVDVGVQVAPDTIIYPNTHLLGDTSIEDDCMIGPNVVLRNTTVGPRCRIVASVLEEASLGADVTVGPFGHLRTDARLDNGVHMGNFGEVKNSYLGPGTKMGHFSYVGDATLGARVNIGAGTVTCNFDGQRKHATIIGDDVFVGSGTMLRAPVVVGAGAKIGAGSVVTHDVPANGLVYGSPARLHGLARPDEVDAQPDDAVSLEERAAGEQGK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc)2. The designed protein have MobA-like NTP transferase domains with specific sequence motifs that are crucial for its activity.3. The designed protein should interact with enzymes and substrates within the Nucleotide-sugar biosynthesis pathway to enhance or regulate its activity.4. The designed protein should be able to compete with endogenous proteins or inhibitors for acetyl-CoA binding.5. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.6. The protein must bind to Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MAAVILAAGMGTRMKSSLPKAMHPLGGRPMLTHLLAAVSAVFDRVIVVVGPGMEALAKLAAPHATVVQEERLGTAHAARMAAPLLAGYAGDVAVLYADNPLISEATLKRLVAARQEAGLAMLAMLTVELPDATGYGRVIRDAAGEVARIVEERDASPAQRAVREVNTGMLAAPARALRDWLARLRNDNAQGEYYLTDIVGLAVADGVPVHTAQPAAAWETLGVNSRVQQAELERIHQRNIAAALLAAWRSAALDAGVTLLAPETVFFSHDTVIANDVLVEQNVVFGPGVRVESGAVIRAFSHLEGCVVRGGAIIGPYARLRPGADVGEGAHVGNFVELKATTLGAGAKANHLTYLGDASIGPGSNIGAGTITCNYDGVNKHRTEIGAGSFIGSNTALVAPVKVGDRALVAAGSTITEDVPADTLALARGRQTVTLAGWRRPVKQPKAE",
    "response#2": "AVILAAGMGTRMKSSLPKAMHPLGGRPMLTHLLAAVSAVFDRVIVVVGPGMEALAKLAAPHATVVQEERLGTAHAARMAAPLLAGYAGDVAVLYADNPLISEATLKRLVAARQEAGLAMLAMLTVELPDPTGYGRIVRNADGDVVGIVEHKDASEAQRAIREIYSGIMGAPTADLKRWLGRLGNANAQGEYYLTDVIGMAAADGVPVVAHRIDDVLQVEGANDRRQLQQLERDYQTRQARALMLSGVTIVDPDNTYIEEGVRIGRDTVIQPGMHIKGATVIGGNCRLGPYGVIEDCRIEDGVEIKFSCCLTSSRVRTGSIIGPFARLRPASDVGPDAEVGNFAEVKKSRIGRGSKMHHHSYVGDTRMGEKVNIGAGVITCNYDGVRKNKTIIGAGAFIGSNTNLVAPVKIGAGAYTAAGSTITENVPGGTLAIARARQATIAGWKRPVKK",
    "response#3": "VVVLAAGEGTRMKSSTPKVLHTLSGRSLVGHVVAAARELDPEHLVVVVGHAREQVRAHLQGIGPDIRTAVQHEQNGTGHAVRMGLEELSDSGVALDGTVIVVCGDTPLLTGETLAALAGTHAADGNAVTVLSAVVPDPAGLGRIVRDADGRLVRIVEERDASDAERAIREINSGVYVFRAGRLREALGRIGTDNAQGEVYLTDVLALARADGAEVAGHRVDDAAAVAGVNDRAQLAAAAAELNRRIVRRWQLEGVTVRDPARLDIRGDVTAGQDVTIDVNVILEGKVVIGDGVTIGPNTIIRDTEIGDGTQIQANCVLEEAVVGKDCRIGPFARLRPQARLADRAHIGNFVEIKKSHVGEGSKVNHLTYIGDAEIGRDVNVGAGTVTCNYDGVNKHRTEIRDAVFIGSGTMLVAPVTIGAGGTIGAGSTITKDTPPDKLTVERSRQRNIAGWVVRRRAGTPSAEAAERALAAGEPETAEDAGDDPAQPGSAQEPQSGQGA"
  },
  {
    "reference": "MKPLKILFAGTPDFAARHLQALIDSEHQVIGVYSQPDRPAGRGKKLQASPVKALALEHDIPVYQPVSLRNEDAQAELAALGADIMVVVAYGLILPQVVLDTPRLGCINVHGSILPRWRGAAPIQRALWAGDAATGVTIMQMDIGLDTGDMLLKTHLPIEDRDTSASLYEKLAEQGPSALIQALKGLAEGSLTPEPQDEALANYAEKLSKEEAQLDWRKPAEQLWREVRAFNPWPASHFPHQDAAIKVWQASVSIEAAKQAPGTIIRADKQGIAVATGEGALVLETIQLPGKKAMAVADVLNARGDWFTPGTQLQGVTPADEAQA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Attaches a formyl group to the free amino group of methionyl- tRNA(fMet)2. The desired protein should be able to perform (6S)-10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) =   (6S)-5,6,7,8-tetrahydrofolate + H(+) + N-formyl-L-methionyl-   tRNA(fMet)3. The designed protein must be able to perform methionyl-tRNA formyltransferase activity under specific conditions. The designed protein sequence is ",
    "response#1": "MKLVFAGTPEVAVPALDALIASDRHEVAAVVTRPDAPAGRGRRLVASPVAERAQEAGIEVLKPAKPRDEEFLARLREIAPDCCPVVAYGALLPKVALDVPARGWVNLHFSLLPAWRGAAPVQHAVMAGDEVTGASTFLIEEGLDSGPVYGVLTEEVRPTDTSGDLLTRLAFAGAGLLAATMDGIEDGTLHAVPQPHEGVTLAPKNISPEEERLDWNKSGRDIFNQIRGMYPWPVAHTLLNGKRFKIYEGDLVEGQGQAGHIIEKTKKSLVVATGQGAISLKSVQPEGKPRMAIADFLNGFKD",
    "response#2": "FMGTPEFSVPTLRLLVDAGHRIVAVYTQPPRPGGRRGLDLQKSPVHQAAELLGLPVFTPVNFKDPEERERFAALKADVAVVVAYGLLLPEAVLNGTRDGCYNGHASLLPRWRGAAPIQRAIMAGDAETGMMVMKMDKGLDTGAVALTRKVEIGPNMTAGELHDRLMQVGAKAMAEKVEVEDARVDWSRPAVVIDHQVRACTPAPGAWTTVAGERLKLGPVTHADAHGSQGLGPGELGVGKHDVLVGTGTTAVRLGDVRPHGRKQMAAADWARGLGKVESRLDWEQPAERLACRVRAFQPWPVAEVVLSGERVRIHEALALDLDHSQPPGTVLAASKEGIDVACVQGALRLCRLQREGGKAITVADYLNAG",
    "response#3": "MRIIFWGTPEYSIASLDIFIKSKHEVIGVVSQPDKKRSRGNKLISSPVKSFAEQESIKIYTPVKIRDNIHFINELKSLSCDLFIVIAYGKILPKEILEIPKFGCWNAHASLLPRWRGAAPIQWSLIKGDKFTGVGIMKMNEGLDTGDLLLEEKIKIGNEDNLNTLSEKLSILSAKLFLKAASLLEENFYKNTKSQLTKQNSLGREITYARMH"
  },
  {
    "reference": "MGDTDVTSDANAPWLIVGLGNPGPEYARNRHNVGFMVADLLAERIGGKFKRAGKAQAQVIEGRIGPPGPANRRVILAKPMSYMNLSGGPVNALREFYKVPVANIVAVHDELDIDYGVLRLKLGGGDNGHNGLKSMTKAMGPDYHRVRFGIGRPPGRMQVADFVLKDFASAERKELDYLVDRAADAAECLVIEGLERAQGTYNS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in translation, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "MF",
    "response#2": "ECC",
    "response#3": "M"
  },
  {
    "reference": "MAMQSETPGNPASTVDPAEIAKFSKLSAEWWDPTGRMAPLHKINPHRVAFIRDAACRKFDRNPKSLNSLAGLRMIDIGCGAGLLCEPFTRLGAQVIGIDPSATNIAAAKVHAEKSRLLIDYRNTTAEAMDPRERFDIVLAMEVIEHVTDVGAFLGRCAAMMKPTGLMVVATLNRNWKSFALAIVGAEYVMRWLPRGTHQWDKFVTPEELTRHLQNSRLAVTEQAGLVYNPLADKWNLSADMDVNYMMVAESAG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway..2. A protein that can perform 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity, 3-demethylubiquinone-9 3-O-methyltransferase activity, 2-polyprenyl-6-hydroxyphenol methylase activity in cytoplasm to support methylation, ubiquinone biosynthetic process.3. The protein have enhanced a 3-(all-trans-polyprenyl)benzene-1,2-diol + S-adenosyl-L-   methionine = a 2-methoxy-6-(all-trans-polyprenyl)phenol + H(+) + S-   adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "MKDIRIATRNAKKLAELRRVLDAAFPEGGRLQLVGLDDVAPFPEAPETGATFAENALIKARAAAAATGEPALSDDSGLTVDALNGEPGIFSARWAGEPKDFGMAMARVREEIAARDDRRATFVACAALVWPDGHEQVARGTLRGEIIDAPRGDGGFGYDPMFLVDGHGLTMAELSPDEKNARSHRGQAFRALAPQLRTALA",
    "response#2": "MPVKVAVISFHSLEDRIVKNFMQRASRGECMCGLPVPACHCGHHHALKVMTKKPITASEDEVAANPRSRSAKLRVAERI",
    "response#3": "MSYERVKDKHPNPFMTESIARLKKENPTKPLITFYDLLWQDDTFKNGVERGEITLPEEKIQDYLKFLSEKFDLDYIDLNPDAVKPDGAWYDMMNDVLSLGIHRIGHEDIWQAHVERGTEIARKLGANLITTYFSPYENRHNAVHPEAEIRKENVKLLRKVAEEVGVKFYGPTEAMPMHATREGILTHPDTHQVWGINPEGDGRVMESHEESYRRNMAFSLFSAARIAGLPWDNTNTVVRIGKVEYGMEEGKEFASKVMRLPEETKVYPTVNHADEIVMWGRASFHPHGIIHWEMAQNVIRGKPFVPVNQAAIWDRDSGKTFTSEGAIDIALKVADYAKSLGAGEILLTSMDRDGTKAGFDYEMTSAISKAVDIPVIASGGAGKMEHFAEAFQKTTVDAALAASIFHYGEITIKEVKEYLRKKNIDVRI"
  },
  {
    "reference": "MAKQFKRKSNGVSESNRKRGRQELRKVTRTAARKQQQDENHEDDLNVNNDSEEDNSGKEIDEEDEEGERDDNRGKAYSALLTLLKSEHKEKPKNVTFGSSSQVEKANASDSEDDGIAGANVDEEEEEGEIDNNVDENEIVSESDDDDDEDATNRLSTDPFESHFNLPSDDYLAKEEKLVLKDNEKWRTVDKQTYSDLAISSLVQLPPGEPLNPPLLKSSKLSEYTIKKRVLDSYEQAYGTGLSDLESTLINPILNYRDVNFQYKSFKNKFYRRLYTLHALNHIFKTRDRILKNTAKLHAAKEDSEELELRDQGFTRPKVLIMLPTRDACYEVVEQLIKLSGTEQQENKKKFNDQFYVKATPPATKPEDFRDAFKGNNNDFFCIGLKFTRKSLKLYSSFYSSDIILASPIGLSMILENPDKKKRQYDFLSSIEVLIVDKCNQIEMQNWDHVNTVMKYLNKVPKEFHDADFSRIRMWSINDQAKLLRQTLVFCEYLTPSINNLISSKSYNLSGKVKFKPIINSENSMMNSIGLKIKQIFQRFDSQSPLQDPDSRYKYFINAILPSLLKTSSYEDGIMIFIPSYFDYLRVKNYLKTSTKFTFGSIDEYSSQSKLTKTRQEFASGKIKLLLYTERLHYFRRYEISGVKTLIMYGLPSNPLFYKELIRFIGKSVFKEECDLDLALVKILFSKWDAVNLEKIVGNERAPVLCNSMNELYEFR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: DEAD-box RNA helicase-like protein required for pre-18S rRNA processing, specifically at sites A0, A1, and A2..2. The ideal protein would be one that plays a critical role in maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) through U3 snoRNA binding, rRNA binding within nucleolus.3. The designed protein's functionality will benefit from Basic and acidic residues, Acidic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "GFETMTEIQQRTIPPTLAGRDILGAAKTGSGKTLAFLIPAVEMLSALRFKPRNGTGVIIITPTRELALQIFGVAKELCEFHSQTYGIVIGGANRRAEAEKLNKGVNLLIATPGRLLDHLQNTQGFVYKNCKVLVLDEADRCLDVGFEAELRQIVKILPSEERQTLLFSATQTTKVEDLARISLKPGPLYINVDHRKEHATVDGVDQY",
    "response#2": "GFGKMTEVQLKCIPEVLKGKDVVVQSPTGTGKTMAFLAPILSCIYDGKGRGRPGVTAVVITPTRELALQIREVAGLFDVKCECFIGGMSIEEDYKRMKEEFSIAVGTPGRLLEIVGKETKKFSSLSHLVLDEADKLLGFGFEEKLMQLLAKLPRNRVTGLFSATRNDSVDKLSRVFLRNPVSINVGNNEMPVALEYIVVR",
    "response#3": "TMKAIRQMGFETMTEIQQRTIPPTLAGRDILGAAKTGSGKTLAFLIPAVEMLSALRFKPRNGTGVIIITPTRELALQIFGVAKELCEFHSQTYGIVIGGANRRAEAEKLNKGVNLLIATPGRLLDHLQNTQGFVYKNCKVLVLDEADRCLDVGFEAELRQIVKILPSEERQTLLFSATQTTKVEDLARISLKPGPLYIL"
  },
  {
    "reference": "MARVKRGVIARKRHNKVLKQAKGYYGARKKIFRVAKQAVIKAGQYAYRDRRNKKRQFRRLWIARINAAARLNGMTYSRFISGLNKAGVEVDRKVLSDIAVHDAAAFSAIVEKAKAALA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that can perform structural constituent of ribosome, rRNA binding in ribonucleoprotein complex, ribosome to support translation, ribosomal large subunit assembly.2. The protein must exhibit the following characteristics: It is not involved in the protein synthesizing functions of that subunit.; Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. The designed protein sequence is ",
    "response#1": "PHNRGVVMNPIG",
    "response#2": "PTVRGSVMNPNG",
    "response#3": "PTVRGSVMNPNG"
  },
  {
    "reference": "MKAILLLAIFSVLTVAICGVSQNYGNVRYNYTELPNGEYCYIPRRRCVTTEQCCKPYDTVNNFAACGMAWPEDKKRKVNKCYICDNELTLCTR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must contain a signal peptide for proper functionality. The designed protein sequence is ",
    "response#1": "RGFGGAMTDAAAINILSLSAGAQEQLLQQYFSSEGIGYSVVRVPMASCDFSTRLYTYADTPGDYNLVNFTLAPEDVDMKIPLLKRAQAMSPQPLHLLASAWSAPAWMKTNGALVGKGSLKGQPGGKEHKAWAQYYIRFLEEYAKYNLTFWALTTGNEPSAGLITNYSFQALGFTPEEQRDWVALDLGPAVRSSSFPQTHILILDDNRLLLPYWATVVLSDVHAARYIHGVAVHWYLDSFVPAEISLGSTHHLYPEYYLFGTEACAGWSTGDRGVKLGSWERAEQYAHDIIQDLNQYVVGWTDWNLALDQAGGPNWVKNFVDSPVVVDAQRDVFYKQPTFYSLAHFSKFY",
    "response#2": "RGFGGAMTDAAAINILSLSAGAQEQLLQQYFSSEGIGYSVVRVPMASCDFSTRLYTYADTPGDYNLVNFTLAPEDVDMKIPLLKRAQAMSPQPLHLLASAWSAPAWMKTNGALVGKGSLKGQPGGKEHKAWAQYYIRFLEEYAKYNLTFWALTTGNEPSAGLITNYSFQALGFTPEEQRDWVALDLGPAVRSSSFPQTHILILDDNRLLLPYWATVVLSDVHAARYIHGVAVHWYLDSFVPAEISLGSTHHLYPEYYLFGTEACAGWSTGDRGVKLGSWERAEQYAHDIIQDLNQYVVGWTDWNLALDQAGGPNWVKNFVDSPVVVDAQRDVFYKQPTFYSLAHFSKFY",
    "response#3": "RGFGGAMTDAAAINILSLSAGAQEQLLQQYFSSEGIGYSVVRVPMASCDFSTRLYTYADTPGDYNLVNFTLAPEDVDMKIPLLKRAQAMSPQPLHLLASAWSAPAWMKTNGALVGKGSLKGQPGGKEHKAWAQYYIRFLEEYAKYNLTFWALTTGNEPSAGLITNYSFQALGFTPEEQRDWVALDLGPAVRSSSFPQTHILILDDNRLLLPYWATVVLSDVHAARYIHGVAVHWYLDSFVPAEISLGSTHHLYPEYYLFGTEACAGWSTGDRGVKLGSWERAEQYAHDIIQDLNQYVVGWTDWNLALDQAGGPNWVKNFVDSPVVVDAQRDVFYKQPTFYSLAHFSKFY"
  },
  {
    "reference": "MSCVHYKFSSKLNYDTVTFDGLHISLCDLKKQIMGREKLKAADSDLQITNAQTKEEYTDDNALIPKNSSVIVRRIPIGGVKSTSKTYVISRTEPVMGTTKAIDDASASISLAQLTKTANLAEANASEEDKIKAMMSQSGHEYDPINYMKKTLVGPPPPSYTCFRCGKPGHYIKNCPTNGDKNFESGPRIKKSTGIPRSFMMEVKDPNMKGAMLTNTGKYAIPTIDAEAYAIGKKEKPPFLPEEPSSSSEEDDPIPDELLCLICKDIMTDAVVIPCCGNSYCDECIRTALLESDEHTCPTCHQNDVSPDALIANKFLRQAVNNFKNETGYTKRLRKQLPPPPPPVPPPRPLMQRNLQPLMRSPISRQQDPLMIPVTSSSAHSAPSISSLTSNPSALAPSVSGNPSSAPAPVPDITATVSISVHSEKSDGPFRDSDNKLLPAAALTSEHSKGASSIAITALMEEKGYQVPVLGTPSLLGQSLLHGQLIPTTGPVRINAARPGGGRPGWEHSNKLGYLVSPPQQIRRGERSCYRSINRGRHHSERSQRTQGPSLPATPVFVPVPPPPLYPPPPHTLPLPPGVPPPQFSPQFPPGQPPPAGYSVPPPGFPPAPANISTPWVSSGVQTAHSNTIPTTQAPPLSREEFYREQRRLKEEEKKKSKLDEFTNDFAKELMEYKKIQKERRRSFSRSKSPYSGSSYSRSSYTYSKSRSGSTRSRSYSRSFSRSHSRSYSRSPPYPRRGRGKSRNYRSRSRSHGYHRSRSRSPPYRRYH",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.2. A protein should be able to manipulate the activity of enzymes in the Protein modification, protein ubiquitination pathway, either by activating or inhibiting their function.3. The designed protein should have nucleic acid binding, ubiquitin-protein transferase activity within chromosome to regulate somite development.4. The protein must exhibit the following characteristics: E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome .5. The protein contains novel DWNN domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "RVSSNGLLCCSSIPEKGVYYVCNPSTREYRKLPKSRERPVTRFYPDGEATLVGLACDLSKNKFNVVLAGYHRSFGQRPDGSFICLVFDSESNKWRKFVSVLEECSFTHMSKNQVVFVNGMLHWLMSGLCYILALDVEHDVWRKISLPDEIRIGNGGGNRVYLLESDGFLSVIQLSDVWMKIWKMSEYETETWSVVDSISLRCIKGLVPGIFPF",
    "response#2": "RVSSNGLLCCSSIPEKGVYYVCNPSTREYRKLPKSRERPVTRFYPDGEATLVGLACDLSKNKFNVVLAGYHRSFGQRPDGSFICLVFDSESNKWRKFVSVLEECSFTHMSKNQVVFVNGMLHWLMSGLCYILALDVEHDVWRKISLPDEIRIGNGGGNRVYLLESDGFLSVIQLSDVWMKIWKMSEYETETWSVVDSISLRCIKGLVPGIFPA",
    "response#3": "MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCG"
  },
  {
    "reference": "MTIISVTLEIHHHITERDADRSLTILDEQLYSFAFSTVHITKKRNGGGSLNNYSSSIPLTPSTSQEDLYFSVPPTANTPTPICKQSMGWSNLFTSEKGSDPDKGRKALESHADTIGSGRAIPIKQGMLLKRSGKWLKTWKKKYVTLCSNGVLTYYSSLGDYMKNIHKKEIDLRTSTIKVPGKWPSLATSACAPISSSKSNGLSKDMEALHMSANSDIGLGDSICFSPSISSTTSPKLNLPPSPHANKKKHLKKKSTNNLKDDGLSSTAEEEEEKFMIVSVTGQTCHFKATTYEERDAWVQAIQSQILASLQSCESSKSKSQLTSQSEAMALQSIQNMRGNSHCVDCETQNPKWASLNLGVLMCIECSGIHRSLGTRLSRVRSLELDDWPVELRKVMSSIGNDLANSIWEGSSQGQTKPSIESTREEKERWIRSKYEHKLFLAPLPCTELSLGQHLLRATADEDLRTAILLLAHGSREEVNETCGEGDGCTALHLACRKGNVVLAQLLIWYGVDVMARDAHGNTALTYARQASSQECINVLLQYGCPDECV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Putative GTPase-activating protein. The designed protein sequence is ",
    "response#1": "AMALQSIQNMRGNAHCVDCETQNPKWASLNLGVLMCIECSGIHRSLGTRLSRVRSLELDDWPVELRKVMSSIGNDLANSIWEGSSQGRTKPTEKSTREEKERWIRSKYEEKLFLAPLPCTR",
    "response#2": "VRKGIPHTFRKELWLRSCPSRADGVWQRHEVPDEVIKQIKLDLPRTFPDNKFLKTEGTRKTLGRALFAVAEHIPSVGYCQGLNFVAGVILLVVNDESRAIDLLVHLVSQRQEYYGKNMIGLRRDMHVLHSLLREHCPRVVVTLEKLDVGLDMLVGKWFVCWFVESLPMETVLRLWDCLIYEGDEWLFRIAVALFRSNMIAISSCR",
    "response#3": "AMALQSIQNMRGNAHCVDCETQNPKWASLNLGVLMCIECSGIHRSLGTRLSRVRSLELDDWPVELRKVMSSIGNDLANSIWEGSSQGRTKPTEKSTREEKERWIRSKYEEKLFLAPLPCTS"
  },
  {
    "reference": "MRSPGRPSVTSPPLRGEIVAAFGRHFLVKTADESTLTCVLRGKKSGAACGDWVEIYQTASEQGVIERILPRSALLYRSDLSREKLIAANATQVIIVVAPVPSFSEELINRCLVAAESQQLSVLIVLNKTDLLGPSKVALNGLSLYRELDYTLLPISARNDVKPLLPYLEDNLSVLVGQSGMGKSTIINALVPDAERATGDISPTLDAGRHTTTHARLYHLDRNARIIDSPGIQQFGLQHLSIEAIAWGFREFRPYIGKCKFNNCRHSGEPGCALANAMRESEVSVRRIDLYHKLIASLNTNRIPSSSSRTKIQ",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The Zn(2+) binding site should be stable and able to withstand changes in environmental conditions.2. The designed protein should have GTPase activity, rRNA binding, metal ion binding, GTP binding to facilitate ribosomal small subunit biogenesis.3. For general function, the protein need meet that Circularly permuted GTPase that catalyzes slow GTP hydrolysis, GTPase activity is stimulated by the 30S ribosomal subunit.; May play a role in the assembly of ribosomal proteins into the subunit; One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit; Helps release RbfA from mature subunits The designed protein sequence is ",
    "response#1": "INRIGRPAVANVSHVLLVISAVEPDFSLELLDRFLTFFSWQKVNVTIYLSKADLISEQRLNEIKESLDYYQKIGYPVFIDYHHLEEKIGDMIKDNQIWTLAGQSGAGKSTLLNHLKKDANQTTGAISTSLNRGKHTTRKVELFKLGHGFLADTPGFSSIS",
    "response#2": "RTCLKRPAVANVEQVIIVFALREPPPDLELLDRLLFLSGVEDIEAVIVWNKADISKQEYAGLPELYRQIGYRNLITSAHTGQGIDELKALLAGRLSTFAGPSGVGKSSLLNAIQPGLNLRTGEVSSKGGRGRHTTRQAELIRLPDGGWVADTPGFSRR",
    "response#3": "MPRSTELCRPNNHGQLKPVAANVDLIVIVFAPAPEPHPNLIDRYLVAAEHAGLRPLLLLNKADLINDENGPGLHALLEVYRELGYPLLEVSAHHGDGMQRLQQQLDGHISVFVGQSGVGKSSLVNSLLPDAGTRVGDLSEWSGQGTHTTTTARLYHFPNGGDLIDSPGIREFGR"
  },
  {
    "reference": "MDLAFSTEENIAAIASAVAPGQGAIAIVKVSGASAKEVVKNVVRTPSNKIWSSHKILYGHVIDKSTKKNIDEVLILIMDGPRSFTGEDVVEIHCHGGLIVVQQVLDAILKQPKTRRAFPGEFSQRAVLNGRLDITQAEAINDLIHARSQKAAQLAIAGIDGDITNKINYLREKLLDQLSEIEARIDFEEDLPKLNSKKLLTDLMLIRAELKQLINDAKQGSLIRNGLKVALVGLPNVGKSSILNLLSKHERAIVTDLPGTTRDLLESEIILEGVPITLIDTAGIRETNNEIEKIGVSLSQKTLFTADIVVLIFDLSKGWNKNDQNLLEKIPKGTPTLIIGNKADLKSQSTNIQPHATMTAITGEGEKELIQELLKLAGANELEGIEVALNQRQLDLVKVAVNALDQIEKVAAESLAWDFWTIDLREAIYKLGELTGEEVTEALLDRIFSRFCIGK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a high specificity for GTP binding, with minimal off-target effects.2. The designed protein must be able to regulate tRNA modification. The designed protein sequence is ",
    "response#1": "KPHVNIGTIGHVDHGKTTLTAAITMSLAAQGKAKARNYADIDAAPEEKARGITINTAHVEYETEGRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLAKQVGVPNIVIFLNKQDMVDDEELLELVELEVRELLSDYDFDGDNIPIVAGSALQAVEALTANPGIGKGENEWVDKILSLMDEVDGYIG",
    "response#2": "KPHVNIGTIGHVDHGKTTLTAAITMSLAAQGKAKARNYADIDAAPEEKARGITINTAHVEYETEGRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLAKQVGVPNIVIFLNKQDMVDDEELLELVELEVRELLSDYDFDGDNIPIVAGSALQAVEALTANPGIGKGENEWVDKILSLMDEVDGYIG",
    "response#3": "KPHVNIGTIGHVDHGKTTLTAAITMSLAAQGKAKARNYADIDAAPEEKARGITINTAHVEYETEGRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLAKQVGVPNIVIFLNKQDMVDDEELLELVELEVRELLSDYDFDGDNIPIVAGSALQAVEALTANPGIGKGENEWVDKILSLMDEVDGYIG"
  },
  {
    "reference": "MKLAVVGVTGLVGRKMLEVLDAYGFTVDELIPVASARSAGQKVEYMGASYTIVEPEQALNLKPDLALFSAGGDVSKHWAPRFAEVGCKVIDNSSAWRMHNDIKLVVPEVNANVLGADDMIIANPNCSTIQMVMALAPLHSKYKIKRLVISTYQSITGTGKDAVDQMNNERKGIAANKVYPHKIDQNCLPHCDEFLDNGYTKEEMKLVNETRKILSDQTIGITATAVRVPVTGGHAESINVTFHNDFDIAEIRELLNNETGMVVEDDPFNNVYPMPINSHERDEVFVGRLRRDESLPNTLNLWIVADNLRKGAATNAVQIAMHLKQKGWL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be optimized for activity in Amino-acid biosynthesis, L-threonine biosynthesis, L-threonine from L-aspartate: step 2/5 pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The designed protein have Semialdehyde dehydrogenase NAD-binding domains with specific sequence motifs that are crucial for its activity.3. The designed protein should have a binding site that is specific to the target substrate, NADP(+), phosphate ligand.4. The protein with the reaction L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH, the Acyl-thioester intermediate, Proton acceptor must be optimized to ensure efficient catalysis.5. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.6. A protein with protein dimerization activity, NADP binding, NAD binding, aspartate-semialdehyde dehydrogenase activity is needed to enhance diaminopimelate biosynthetic process, 'de novo' L-methionine biosynthetic process, threonine biosynthetic process, isoleucine biosynthetic process, lysine biosynthetic process via diaminopimelate in a specific context. The designed protein sequence is ",
    "response#1": "MGYKVAVVGATGNVGREMLDILSERAFPADEVVAIASRRSAGQDVSFGDKILKVKALDSYDFSDTDICLMSAGGETSKEWSPRIGQSGCVVIDNSSAFRYHQDVPLIVPEVNPDAINGRVAVVGATGNVGREMLNILAERKFPASEVIALASARSQGQEISFGDKRLKVQNLEHFDFKGVDFALMSAGSAIAKEYGERIGATGCIVVDNSSFWRYHGDVPLVVPEVNAHVLDADAIIANPGCTTAQMVVALKPLHDEAGLQRLIVSSYQAVSGSGLAGVEELATQARAVIGDAEKLTHDGSAVPFPAPNKYVAPIAFNVLPLAGSIVDDGSGETDEDQKLRNESRKILGLPDLLVSGTCVRVPVFTGHSLSINAEFARPLSVERAKELLADAAGVKLVDVPTPLAAAGKDESLVGRIRQDPGVPEGRGLALFVSGDNLRKGAALNAVQIAELLVRRGLQA",
    "response#2": "MRVAVVGATGNVGREMLNILAERKFPASEVIALASARSQGQEISFGDKRLKVQNLEHFDFKGVDFALMSAGSAIAKEYGERIGATGCIVVDNSSFWRYHGDVPLVVPEVNAHVLHPLRRENSILRIVADTYQSVSGAGGAAMTELESQSRQLLEGKEKVQAEALDQQIAFNVFPHIDRFLDDGYTNEERKMMQETRKIMHAPDIAISATCVRVPVYVSHSAAVHLEFERPMSPEEAREILEATPGVTVLDDPSRAQYPMPKDVAGADDVFVGRIRRDASHPNGLVMWVVSDNLRAGQIIANPNCSTIQMVAALKPLHDAATIKRVVVSTYQSVSGSGKEGMDELFEQTRAVFVADPISAKKFTKRIAFNVIPHIDVFMEDGYTKEEWKVLAETKKMLDPKIKVTATAVRVPVFIGHAESVNLEFEKPITADEARDILREAPGCQVIDKREDGGYITPYESAGEDATYISRIREDATVENGLALWVVSDNLRKGAATNTVQIAELLVRRGWL",
    "response#3": "MSVAILGATGAVGTRLIEQLEQSTIPVKSIKLLASSRSAGKILQFQGQNVVVEEATPESFTGVDLVLASGGGSVSARFAPEAIKRGAVVVDNSSQWRMNDNVPLVVPEVNEEALKNRPKGIIANPNCTTLSMMAALKPLHDKYKIKRVVVSTYQSVTGTGVKAVDQLFNERNGIDGPKVYPYTIDLNVIPQIDVFTENGYTKEEMKMILETKKIMGDDSIQVTATTVRIPVMGGHSESVNIEFLHDFDLSEVRELLANSPGIVVVDDIANLKYPMPLDAHDKDDVFVGRLRRDESQEKTLNCWIVSDNLRKGAALNTYEIAEELLYPLHKVNPIKRIVVDTYQAVSGTGSAAVDELNSQTKLILEGKSIVPHVYPHQIAFNLLPEIDVFLDDGYTKEEWKVIHETRKILHDESIAVSATCVRVPVVTGHSEAIHIEFSNPMSPEEARDILATAPGVKVLDDTPISLYPHPWAAAGTDEVFVGRIRRDASHPNGLAIWVVADNLRG"
  },
  {
    "reference": "MSWFNYFRATRGNSARTAKERLQIVIAHERIDRSGPSYLPRLRGDIIEVIRKYIEIDEDQVKIQMEQEGDMDVLALNIQLPDASPPLSETRHSSKQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Prevents the cell division inhibition by proteins MinC and MinD at internal division sites while permitting inhibition at polar sites; This ensures cell division at the proper site by restricting the formation of a division septum at the midpoint of the long axis of the cell.2. The protein design must be focused on enhancing the efficiency of regulation of division septum assembly, cell division, cell cycle. The designed protein sequence is ",
    "response#1": "MVL",
    "response#2": "SCGGR",
    "response#3": "MRFGL"
  },
  {
    "reference": "MAVKYVFVTGGVVSGLGKGITAASLGRLLKNRGYKVTIQKFDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLSVDSNITTGKVYWNVLNKERRGDYLGRTVQVIPHITNEIKNKVYAVGKNSNCDVVITEIGGTVGDIESTPFLEAIRQVVTEVGRENVIYIHVTLVPTVPGSTELKSKPTQHSVKELLSLGIQPNVIVCRSDYPLPDDMVNKIALFCNIRPQDVIQNLTAGTLYEVPMMLEKNGLVKSVCHHLHLEEREPDHIQWLQMIDQVKSANKEVTIGLVGKYVELPDAYISVAESLYHGGLPHSTKVKIEYIQSEDITEDNCADLLKNCDGILVPGGFGDRGIEGKITAIRYCRENNLPFFGICLGMQMAVIEFARHVAGLENANSAEFLEDAEDPVIDIMDDQKDITKKGGTMRLGLYPCKLRPDTKCRNLYGEEVIYERHRHRYEFNNAYRAKLEEAGLTLAGVSPNEKLVEIVEVKEHPWFVGVQFHPEFKSRPNRPHPLFVDFVGAALQHKENQ",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design must be able to perform glutaminase activity, metal ion binding, ATP binding, CTP synthase activity in order to contribute to glutamine metabolic process, 'de novo' CTP biosynthetic process.2. The protein should have a high affinity for Mg(2+), CTP, L-glutamine, ATP, UTP.3. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen4. The protein contains novel CTP synthase N-terminal, Glutamine amidotransferase domains that confer a unique function or activity.5. The protein design should take into account the physiological conditions of the Pyrimidine metabolism, CTP biosynthesis via de novo pathway, CTP from UDP: step 2/2 pathway.6. The protein with the reaction H2O + L-glutamine = L-glutamate + NH4(+), the Nucleophile; for glutamine hydrolysis, Nucleophile, active site must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "KFIFVTGGVVSGLGKGITASSLGALLKASGLKVFMQKFDPYLNVDPGTMSPYQHGEVFVTSDGGETDLDLGHYERFTDEELSKISSTSAGKIYLEVIEGERRGDSQGKTIQVVPHITDAIKHKVYLAAQRSKADVIISEIGGTVGDIESQPFIEAIRQIRMEQGKENVMFIHVVLLLWLAASKEYKTKPIQHSVRELLSLGIQPDVIVSRSDKSSPQEIKEKVSLFCNVPTENIIDAVDQDSIYRVPLAMAEQNLHKLVIDQLKLKDTYFSVTESLKIAGIWQGVKTEVDWIDSEELENLPPKKIAAKFKEYDGILVPGGFGSRGIEGKISAIKYAREHKKPFLGLCYGMQLATIEFARHVAGLKDANTTEVNPKTSHPVIHIMPDQEKKMLKNDYGGTMRLGAYPCKIIDNTKTAKAYGEKNISERHRHRYEFNNEYREILTKKGLVISGTSPDDLLVEIIELKDHPFFIATQFHPEFKSRPLKPHPLFYEFVKESYKSLNEALLHGGIANDARVNLTFIDSEKLSKDNVADTLHPCDALLVPGGFGERGVEGKILAIEYARTHDVPFFGICLGMQLMAIEFARNVCGLKDANSTEFKEAPEHPVITLMADQKNIEKKGATMRLGAYPCKLSAGSKARAIYQSENISERHRHRLEFNNAYREKLSENGMSFCGQSPDGHLVEMMELPEHSWFIGCQFHPEFKSRPTAAHPLFESFVISIAVVGKYIKLQDSYKSVYEALRHAAIANKVELNIMKIDSEEIEKNPENLTKVINQADGILVPGGFGSRGIEGKLAIIQIAREKKIPYFGICLGMQCMVIEYARNVLKIADANSSEFTETSNPVIHLMEDQYSIDRKGGTMRLGVYECNIKKGTLAEKIYGENIIFERHRHRYEFNNKYEDQFEKAGLIISGKYITKNLVEIVEYKDNPFGIGVQFHPEFKSKPFKPHPIFKSF",
    "response#2": "KYIFVTGGVVSGLGKGITAASLGRLLKARGLKVAAQKLDPYINVDPGTMSPYQHGEVFVTNDGAETDLDLGHYERFIDEDLNKYSNLTTGKVYWNVINKERSGEYLGETVQVIPHITNEIKSFVYEVGKKTDADVVITEIGGTTGDIESQPFIEAIRQISLEVGLSNCLFVHVTLIPYIQSSGEHKSKPTQHSVKELRSIGIMPNIIIARCDEPIDDSIRHKIAMFCNVKLDCVIQNITLPVLYEAPIMLDKENFSDIVCRELSLNADDVDLNVWRELVRRIQSPTSRISIAVVGKYIKLQDSYKSVYEALRHAAIANKVELNIMKIDSEEIEKNPENLTKVINQADGILVPGGFGSRGIEGKLAIIQIAREKKIPYFGICLGMQCMVIEYARNVLKIADANSSEFTETSNPVIHLMEDQYSIDRKGGTMRLGVYECNIKKGTLAEKIYGENIIFERHRHRYEFNNKYEDQFEKAGLIISGKYITKNLVEIVEYKDNPFGIGVQFHPEFKSKPFKPHPIFKSFIKAALDH",
    "response#3": "RFIFITGGVVSALGKGIAAASIGQLLVSRGLTVTLQKFDPYINVDPGTMSPFQHGEVFVTEDGAEADLDLGHYERFTDVNATRASNATAGAIYDSVIRRERRGDYLGGTVQVIPHITDEIKHRIKLVAETDEVDVVITEIGGTVGDIESLPFLEALRQFSVDLGRERCMFVHLTLVPFLGHAGELKTKPTQHSVQELRRIGIQPDMVMCRSEETLGRDIREKIALFANLPVESVISARDVRSIYRVPLEFRKEGVDDRVLSHFLQDAYKSLNEALVHGGIANRVKVNIRWIDAEIFEHEDSDVAASLEPLHAILVPGAFGERGAEGKIASVKFARERQVPYFGICFGMQMACIEGARNLAGIEKASSTEFGPTDEPVVGIITEWMTAEGVEKREAGGDLGGTMRLGAYDAKLAGNSVVSSIYGATDISERHRHRYEVNTGYRTALEKGGLVFSGMSPDGMLPEIVERPDHPWFVGVQFHPELKSKPFDPHPLFASFIRDADKSLCEALTHGGIANDTRVNIEWIEAESFEKEPHTVAERLKDKKAILVPGGYGERGTAGMISAIQYAREHQIPYMGICFGMQLAVIEVARHLAGIEKANTTEFGVTAEPVVGLLTEWLNGDVKERRSAEDDLGGTMRLGLYPAILAPGSLIQKIYGTSSIQERHRHRYELNIEYREKFEKAGLKFSGLSPDGLLPETVELEGHPWFVGVQFHPELRSRPFDPHPLFTSFIKNWS"
  },
  {
    "reference": "MNEKYSALRSNVSMLGHMLGNTIKDAHGEPLFDKVETIRKLSKSARAGNDSDRAKLIDELQNLPDDQLLPVARAFSQFLNLTNIAEQYHTISRHCEEQICSPDSIDSLFGKLNANSISQLDSIQAVRDLNIELVLTAHPTEIARRTMIHKLVQINKCLSNLELSELSHSERQRVEQRLEQLIAQAWHSDVIRKQRPTPLDEAKWGFAVVENSLWHAVPEFLRQFDEKLMRHLGEKLPLDASPVKFTSWMGGDRDGNPFVTSEVTREVMLLSRWKAADLFLGDIQELVSELSMVKCNDQVRELAGEEHEPYRAILKDLRTKLTNTRDVLAAKINKQDAPNLATIDDVDQLWTPLHACYQSLRECGMGIIADGLLLDVLRRIKCFGIHLVRLDIRQESTRHSDVISELTRYLGLGDYEQWNEQDKVAFLVRELASKRPLLPLAWQPSAEVQEVLDTCKAVAEQSREALGAYVISMARTASDVLAVHLLLKEAGCPFRLDVCPLFETLDDLNNGASVIKELMSIDWYRGFIQNHQMVMIGYSDSAKDAGVMAAGWAQYRAMEELVEVCEDASVELTLFHGRGGSIGRGGAPAHAALLSQPPRSLKGGLRVTEQGEMIRFKLGLPDVAVNSLSLYASATLEANLLPPPAPKKEWCDLMDVLSEVSCESYRNVVRGSEEFVPYFRAATPELELGKLPLGSRPAKRNPNGGVESLRAIPWIFAWSQNRLLLPAWLGAGEAIQYSIDKGHAQLLEDMCREWPFFSTRLGMLEMVY",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design must be able to perform phosphoenolpyruvate carboxylase activity in order to contribute to tricarboxylic acid cycle.2. The protein's active site must be carefully engineered to ensure that the amino acids involved in oxaloacetate + phosphate = hydrogencarbonate +   phosphoenolpyruvate are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "LMVSFTGYALYTAFGQPS",
    "response#2": "MFLPNVSFLL",
    "response#3": "GFI"
  },
  {
    "reference": "MSIPGLGQIAPQQPTTSTTRTITLRPFWEWRFEVPRSSIPTTNAAISAIGLGGAGAGGGGATVRLTSGTAERDGTELALNRTYTFPRNTQSKLLTYTGATLEVSGAFVDSVAQYPAPEASPQLPVLNLHFALQELRAAAAAGGSNHNNNNTNGGGAPGPRVMICGEKDSGKTTVARTLAALATRAGGQPLVGSVDPREGMLALPGTVSAAVFGTVMDVEDPAAGFGVSGTPSSGPSAVPVKLPMVYYVGRERVDEDVPLWRDLVGKLGSAVRDKFAADEVVREAGLLLDTPAASVAKGDLEVLTHVVNEFAGGLLGAGRTAGWQLTVTVNIVVVLGSVDLHAELQRRFENQRTVHGEAITLILLDKSDGVAERDKDFMKFTREAAIKEYFFGDAKRTLSPFTQSVSFDDVAVFRTPDALERAEVSAEMSHWTLAVMNASVNDPPEVIRQAPVMGFVAIADVDEDRRRLKVLSPVSGRLGNRPMIWGRWPEPYINLLG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Required for endonucleolytic cleavage during polyadenylation- dependent pre-mRNA 3'-end formation.. The designed protein sequence is ",
    "response#1": "LYPHSFEVKFSDVKLFKIGAPLVPDSCLPLGMDQGQNETKLVPVVPTKDLKHCLLAISAAESLEEDLVQTNVIGFLVVNEVDLDREVMVVLSPAPRPLPRKFLLLSEIKFMK",
    "response#2": "GPTDVGKSTVCQLLLNYAVRMGRRPISVDLDVGQGTASVPGSMGALLLERPADIEEGFSLQAPLVYLFGHTSPSPNEKLYNMLSSKIADIVFQRFERNKKACASGCVINTCGWVTGMGYRIIVHAATAFKVNVIVVLDQERLYNDLKNQFGDKVQIVHLPKSGGVVVRSRETRRKVRDERLRSYFYK",
    "response#3": "LYPHSFEVKFSDVKLFKIGAPLVPDSCLPLGMDQGQNETKLVPVVPTKDLKHCLLAISAAESLEEDLVQTNVIGFLVVNEVDLDREVMVVLSPAPRPLPRKFLLLSEIKFMC"
  },
  {
    "reference": "MDDNKRPLYLPFAGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERILGLGDQGIGGMGIPIGKLSLYTSCGGISPAYTLPITLDVGTDNPQLLEDPMYMGWRHPRIGGEEYAEFIEAFMQAVHVRWPDTLIQFEDFAQKNAMPILERYKERYCCFNDDIQGTAAVTVGSLLAACKAAGTELNKQRVAFLGAGSAGCGIAEAIVAQMVSEGISDEQARSQVCMVDRWGLLLDNMPNLLPFQQKLAQKCADISHWNNFSDNISLLDVVNNAKPTVLIGVSGAPGLFTEEIVRAMHSHCPRPIIFPLSNPTSRVEATPKDILHWTSGQALVATGSPFEPVVVDGETYEIAQCNNSFIFPGIGLGVLASGARHVSDAMLMASSRALAECSPLAINGSGPLLPKLEDIHSVSKHIAFAVGKVAIEQGLSLPASDELLMQSIEDNFWKPEYRRYKRTSF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must have a Proton donor, Proton acceptor active site that is highly conserved among related enzymes.2. The protein should be able to modulate malate metabolic process in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "FKVFREFTSDDSFLNQVIPENITEFQVTLSLARDYDGNNSTNGKFIPYWDTEKVTPEVIKKFKKKYEPTALRVKVLVSIGNKNKQFPFTIGSDSNSEAWVSEATASLKSIIKTYNLDGIDVSYEDIAANEADFVNSVGGLVRNLKQNKLITVASFATSADAANNKFYNLLYAEYATFFDTVVFLSWVGFTPSRANPVASLEEKILAVANEYKAVKAFLVAYSTVAEDWANFSPPIFFITLHGLLGNSAVKGASIKVISDATASFPAKWIPEILLLAASK",
    "response#2": "FKVFREFTSDDSFLNQVIPENITEFQVTLSLARDYDGNNSTNGKFIPYWDTEKVTPEVIKKFKKKYEPTALRVKVLVSIGNKNKQFPFTIGSDSNSEAWVSEATASLKSIIKTYNLDGIDVSYEDIAANEADFVNSVGGLVRNLKQNKLITVASFATSADAANNKFYNLLYAEYATFFDTVVFLSWVGFTPSRANPVASLEEKILAVANEYKAVKAFLVAYSTVAEDWANFSPPIFFITLHGLLGNSAVKGASIKVISDATASFPAKWIPEILLLAASK",
    "response#3": "VTPEVIKKFKKKYEPTALRVKVLVSIGNKNKQFPFTIGSDSNSEAWVSEATASLKSIIKTYNLDGIDVSYEDIAANEADFVNSVGGLVRNLKQNKLITVASFATSADAANNKFYNC"
  },
  {
    "reference": "MAENKYIVGSRDDSDFTTWTAAELAEKIHAREISSQEVTQAHLDRIGEIDGDIHAFLHVGADEALAAASNVDDALAAGDQPTSKLAGVPLALKDVFTTTDAPTTCASKMLEGYMSPYDATVTMRLRAAGIPILGKTNMDEFAMGSSTENSAYGATKNPYDLERTPGGSGGGSSAALAAGMAPLAIGTDTGGSIRQPAALTNTVGVKPTYGTVSRYGLVACASSLDQGGPTARTVLDTALLHEVIAGHDANDSTSSSHAVAPVVEAAKQGASGDLSGVKLGVVKQFEKAEAFQPGVLETYHANLEQLKSQGAELVEVDCPNFDHALNAYYLILPCEVSSNLARFDGMRYGQRRGDDGTRSADQVMSLTRAEGFGPEVKRRIMLGTYALSVGYYDAYYLQAQRVRNLISQDFAKAYEQVDAIVAPVTPSTAFKLGEKVDDPLAMYMFDLFTLPLNLAGVCGMSVPGGFASDSGLPTGLQIMGPAHGDDRLYRVGAAFEAGRS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein with the reaction ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) +   L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate, the Charge relay system, Acyl-ester intermediate must be optimized to ensure efficient catalysis.2. For general function, the protein need meet that The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln).; Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase3. The designed protein should be involved in translation by localizing to glutamyl-tRNA(Gln) amidotransferase complex and exhibiting ATP binding. The designed protein sequence is ",
    "response#1": "ELVKAQLAEIEQARALNAFIKETPEKALAMAEASDARIARGEAGPLEGVPLGIKDLYCTEGVETTAASHILEGFKPAYESTVSANLWRDGAVMLGKLNLDEFAMGSSNETSYFGPVVSPWRRKGSDARIVPGGSSGGSAAAVAARLCFGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPIARTVRDAAILMRSMAGHDPKDTTSVDAPVPDYEATLSRGVKGMKIGIPKEYRIEGVPAEIDALWGQGVEWLKDAGATIVDISLPHTRYALPAYYIVAPAEASSNLARYDGVRYGLRVPGKDIVGMYEATRAAGFGKEVRRRIMIGAYVLSAGYYDAYYVRAQKIRTLIKQDFDRAYADGVDAILTPATPSAAFGFGEKGSGDPVEMYLNDVFTVTVNMAGLPGLAVPAGVSAEGLPLGLQLIGRPFDEETLYRVAHAFEEQTGRLRS",
    "response#2": "ELVKAQLAEIEQARALNAFIKETPEKALAMAEASDARIARGEAGPLEGVPLGIKDLYCTEGVETTAASHILEGFKPAYESTVSANLWRDGAVMLGKLNLDEFAMGSSNETSYFGPVVSPWRRKGSDARIVPGGSSGGSAAAVAARLCFGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPIARTVRDAAILMRSMAGHDPKDTTSVDAPVPDYEATLSRGVKGMKIGIPKEYRIEGVPAEIDALWGQGVEWLKDAGATIVDISLPHTRYALPAYYIVAPAEASSNLARYDGVRYGLRVPGKDIVGMYEATRAAGFGKEVRRRIMIGAYVLSAGYYDAYYVRAQKIRTLIKQDFDRAYADGVDAILTPATPSAAFGFGEKGSGDPVEMYLNDVFTVTVNMAGLPGLAVPAGVSAEGLPLGLQLIGRPFDEETLYRVAYAYEQATAWHTKRPSL",
    "response#3": "ELASQYLDRIEALNPQLNAIVTVDREKTLAEARAADARLAAGDARALTGVPLVHKDLFCQQGWKTSCGSRMLDNFVSPYSAHVVEQCAAAGMVTLGRANMDEFAMGSSNENSFYGAVKNPWDLNAIPGGSSGGSAAAVAARLAPVATATDTGGSIRQPASHCGVTGIKPTYGVVSRYGMVAYASSLDQGGPIAQTAEDCALMLNVMAGFDARDSTSLERAKEDYARDLNQSLSGLKVGLPKEYFAAGLDADVARAVDNAVAELKKLGAEAVEISLPNTELSIPAYYVIAPAEASTNLSRYDGVRYGHRAKDYKDLVDMYEKTRAEGFGDEVKRRILVGSYVLSHGYYDAYYLKAQKIRRLIANDFKAAFEQCDVILGPVAPTAAFNIGEKSGDPVQMYLSDIYTLSVNLAGLPGMSVPAGFAANGRPIGLQIIGNYFAEARMLRAAHAFQQVTEWHTRTPAGF"
  },
  {
    "reference": "MAEQFADAVIVAAGSSRRMEGVDKLAAPLAGRPVLAWAIDSMRKARSVRRIVVVTAAERLDELARQPWLREAGAVLAPGGAERQGSVASGVAQTDASIVLVHDGARPFVRADVVDAVADAARRHGAAIPILPVADSLKHLDVAGRALATDRDGLYRAQTPQGAHRDLLIAAHEAAEASGRQYGDEAALLEAHGIPIATVRGDAANLKVTEPDDLLLIGAIASARVGSPRYASATDSHPFGPEDGLMLGGLLIPEAPRLFGHSDGDVVLHAVSDALFAAAGLADLGRQAPASDPSTRGIASNELLRMAIERSGSAGWRTVSADVSILGSRPRLGGARLDEIRRLLAGLLAIPVEQVSIKASTGNLGGPEGAGRAITATALVGVMPR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein have enhanced 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-   erythritol 2,4-cyclic diphosphate + CMP.2. A protein with 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity, metal ion binding, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity functions is required to enable terpenoid biosynthetic process.3. The protein must exhibit the following characteristics: Catalyzes the conversion of 4-diphosphocytidyl-2- C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).; Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds.4. The designed protein should have a binding site that is specific to the target 4-CDP-2-C-methyl-D-erythritol 2-phosphate, a divalent metal cation ligand. The designed protein sequence is ",
    "response#1": "MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDDRYKGISSIKLLEEVGKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADALKIDVDRVNVKATTEEGLGFTGSGEGISSQAICSLNAKAEAMTSPALSSRAASGFDVHRFGPGDHVWLCGVQIPHSHGLIGHSDADAGLHALTDALLGTIAAGDIGDHFPPSDPQWRGAASDTFLAHAATLVHAAGGIIDHADVTLICERPKIGPHRAAMRARIAAILGIDVARVSVKATTTELLGFTGRSEGLAAQAMASG",
    "response#2": "MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDDRYKGISSIKLLEEVGKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADALKIDVDRVNVKATTEEGLGFTGSGEGISSQAICSLNAYTALAAGVRTGTGYDVHALGEGDGIWLCGVHIDHDKRLVGHSDADVALHALTDALLATLGDGDIGTHFPPSDPQWKGAASKIFVAHAVEKVRARDGRIANADITLICEAPRVGPHRAEMTQALHEMLGISAGRISIKATTNEKLGFIGRSEGIAAFATVSVVRR",
    "response#3": "MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDDRYKGISSIKLLEEVGKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADALKIDVDRVNVKATTEEGLGFTGSGEGISSQAICSVSGPARRLGGAEALIPRQIRVGQGFDAHRWGPGTSVWLCGVEIAHDQTLIGHSDADAGLHALTDAILGAMGDGDIGDHFPPSDPQWKGASSDLFLIHAVERLTARGGRLVNVDVTLICERPKVKPHRQAMRERLADILSLPLDAVSVKATTTEAMGFTGRGEGLAAQALATVRTGTGYDVHALGEGDGIWLCGVHIDHDKRLVGHSDADVALHALTDALLATLGDGDIGTHFPPSDPQWKGAASKIFVAHAVEKVRARDGRIANADITLICEAPRVGPHRAEMTQALHEMLGISAGRISIKATTNEKLGFIGRSEGIAAFATVSVVRVGVGIDVHAFDDSSPLWLAGLHWPGERGLSGHSDGDPVAHAVCDALLSAAGLGDIGSRFGTADPRFQDAHGEVFLRATRDLVEAAGFTIGNVSVQLIGNRPRFSPRREEAESLLSALLAAPVSVAATTSDALGFTGRGEGVTAVATALVT"
  },
  {
    "reference": "MEPVDPNLEPWNHPGSQPTTPCSKCYCKKCCWHCQLCFLKKGLGISHGRKKRKHRRRTPQSSEDHQNSIPKQPLSIIRGNPTGPKESKKKVASKAETDPCDL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have a binding site that is specific to the target Zn(2+) ligand.2. The designed protein should be involved in negative regulation of peptidyl-threonine phosphorylation, suppression by virus of host type I interferon-mediated signaling pathway, positive regulation of viral transcription, suppression by virus of host translation initiation, positive regulation of transcription elongation by RNA polymerase II by localizing to host cell nucleolus, host cell cytoplasm, extracellular region and exhibiting trans-activation response element binding, protein serine/threonine phosphatase inhibitor activity, RNA-binding transcription regulator activity, cyclin binding, metal ion binding.3. The protein design should include a Nuclear localization signal, RNA-binding (TAR), and protein transduction motif to ensure proper function.4. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "MENRWQVMIVWQVDRMRIRTWNSLVKHHMYVSKKAKDWVYKHHYESRHPKVSSEVHIPLGDARLVITTYWGLHTGERDWHLGQGVSIEWRKRAYSTQLDPDTADQLIHLYYFDCFSESAIRNAIVGRIVSPRCNYQAGHNKVGSLQYLALTALIAPKKIKPPLPSVRKLTEDRWNKPQKTKGHRGSHTMSGH",
    "response#2": "MEPVDPSLEPWKHPGSQPKTPCTNCYCKKCSYHCQVCFITKGLGISYGRKKRRQRRSAPPSSEDHQNLISKQPLSRSQRDQTGSEESKKKVESKTETDPCD",
    "response#3": "MYLFILLLILSSGISAASVKTEETCKDNTECRHLCCKNEEGKLVCSPKYRILLEIFQRLQNLHY"
  },
  {
    "reference": "MKNSEKLKILLASSNPGKIQEIIALSKKLPIEWIPQSEFNIEDTEETGKSFIENAILKARHAAKISGMPALADDSGLVVDALDGAPGIFSARYAGKTATTEERNQKLLTELKDIEEADRTAAYHCVLALVETENDPVPLICHGVWEGSILFEPAGENGFGYDPIFYVPTHQCSAAELSTAEKNRISHRGQVMQQLIDVFKSANEP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be able to regulate nucleotide metabolic process.2. The Proton acceptor of the protein must be engineered to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "GFKC",
    "response#2": "LFT",
    "response#3": "MR"
  },
  {
    "reference": "MECCLSEEAKEQKRINQEIEKQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGYIKLVFQNIFMAMQSMIKAMDMLKISYGQGEHSELADLVMSIDYETVTTFEDPYLNAIKTLWDDAGIQECYDRRREYQLTDSAKYYLSDLARIEQADYLPTEQDILRARVPTTGILEYPFDLDGIVFRMVDVGGQRSERRKWIHCFENVTSIIFLVALSEYDQILFESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPKQDHAAAKQFVLKKYLACNPDPERQCYSHFTTATDTENIKLVFCAVKDTIMQNALKEFNLG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a binding site that is specific to the target GTP ligand.2. The ideal protein would be one that plays a critical role in mucosal immune response through GTPase activity within heterotrimeric G-protein complex. The designed protein sequence is ",
    "response#1": "GNRLGTLVQTYVNAINSGTVPCLENAVTTLAQRENSIAVQKAADHYSEQMAQRMRLPTDTLQELLTVHAACEKEAIAVFMEHSFKDDEQEFQKKLVVTIEERKEEFIRQNEAASIRHCQAELERLSESLRKSISCGAFSVPGGHSLYLEARKKIELGYQQVLRKGVKAKEVLKSFLQSQAIMEDSILQSDKALTDGERAIAAERTKKEVAEKELELLRQRQKEQEQVMEAQERSFRENIAKLQEKMESEKEMLLREQEKMLEHKLKVQEELLIEGFREKSDMLKNEISHLL",
    "response#2": "GGVIVTGNRLGTLVQTYVNAINSGTVPCLENAVTTLAQRENSIAVQKAADHYSEQMAQRMRLPTDTLQELLTVHAACEKEAIAVFMEHSFKDDEQEFQKKLVVTIEERKEEFIRQNEAASIRHCQAELERLSESLRKSISCGAFSVPGGHSLYLEARKKIELGYQQVLRKGVKAKEVLKSFLQSQAIMEDSILQSDKALTDGERAIAAERTKKEVAEKELELLRQRQKEQEQVMEAQERSFRENIAKLQEKMESEKEMLLREQEKMLEHKLKVQEELLIEGFREKSDMLKNEISHLL",
    "response#3": "GGVIVTGNRLGTLVQTYVNAINSGTVPCLENAVTTLAQRENSIAVQKAADHYSEQMAQRMRLPTDTLQELLTVHAACEKEAIAVFMEHSFKDDEQEFQKKLVVTIEERKEEFIRQNEAASIRHCQAELERLSESLRKSISCGAFSVPGGHSLYLEARKKIELGYQQVLRKGVKAKEVLKSFLQSQAIMEDSILQSDKALTDGERAIAAERTKKEVAEKELELLRQRQKEQEQVMEAQERSFRENIAKLQEKMESEKEMLLREQEKMLEHKLKVQEELLIEGFREKSDMLKNEISHLV"
  },
  {
    "reference": "MKIILATHNRDKEKELQNSLQGINVEICSLFDFPEIGDIEETGTTLLENSLLKARTVFNITGIPTIADDTGLEVDFLDGAPGVYSARYAGNNVSYQDNVNKLLIELDGVPREKRKAKFRTVVTYIDKNDELWTEGHIDGIISESIIGDGGFGYDPIFFVPHIGKTFAELSSAEKNKISHRGIALQKLRKILINVLK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..2. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.3. The designed protein should have ribonucleoside triphosphate phosphatase activity, dITP diphosphatase activity to facilitate purine nucleoside triphosphate catabolic process.4. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+).5. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "MRDPAEIIADTRASLDAGRAPPA",
    "response#2": "MTKRFVLSVDGGGIRGIIPATVLEEVERRGGLPVCDLFDMIGGTSTGGILALGLATPDDNSRPRYTAKDIVDLYFEYGRQIFSQRTWSLGLSRLWSARYDSKPLEKILRERFGDTTLGDTLTDLVIPSYDIHRRYPVMFSSWHADHHPRELPLHDACLATSAAPTYFPPARIDGTLYVDGGVWANNPAICGVSEALHVYQRPPKIDVLSLGTGTVRDYPPAEAAKCWGLLQWARPIVNMVMHGASETTDHVADFLLRDYYHRLNPPLSVDNALDDASAAKLDELGRKTLTYMHSDDELAALAGLLKP",
    "response#3": "MPIIKIDNVLYPSDTEALAAALKDAAPEKS"
  },
  {
    "reference": "MDSKGRNVIVCDNGTGFVKCGYAGSNFPTHIFPSMVGRPMIRAVNKIGDIEVKDLHVDDLMVGDEASQLRSLLEVSYPMENGVVRNWDDMCHVWDYTFGPKKMDIDPTNTKILLTEPPMNPTKNREKMIEVMFEKYGFDSAYIAIQAVLTLYAQGLISGVVIDSGDGVTHICPVYEEFALPHLTRRLDIAGRDITRYLIKLLLLRGYAFNHSADFETVRIMKEKLCYIGYDIEMEQRLALETTVLVESYTLPDGRVIKVGGERFEAPEALFQPHLINVEGPGIAELAFNTIQAADIDIRPELYKHIVLSGGSTMYPGLPSRLEREIKQLYLERVLKNDTEKLAKFKIRIEDPPRRKDMVFIGGAVLAEVTKDRDGFWMSKQEYQEQGLKVLQKLQKISH",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be able to bind ATP ligand in a variety of conditions2. The ideal protein would be one that plays a critical role in cell morphogenesis through structural constituent of cytoskeleton within actin filament.3. For general function, the protein need meet that Seems to contact the pointed end of the daughter actin filament . The designed protein sequence is ",
    "response#1": "VGVSDLTLISKVSNEAINENLKKRFDNREIYTYIGHVLVSVNPFRDLGIYTDAVLESYKGKNRLEMPPHVFAVAESAYYNMNAYKDNQCVIISGESGAGKTEAAKRIMQYIANVSGGSNSSIQETKEMVLATNPLLESFGNAKTLRNNNSSRFGKYLQLQFNAQGEPVGADITNYLLEKTRVVTQIKDERNFHIFYQFTKGASQAYRESYGIQQPSQYLYTSKAGCFDVDGIDDLAEYQDTLQAMKVIGLSQAEQDEIFRMLAAILWTGNIQFREGDDGYATVVDQSVVDFLAYLLDVDAAHVIQAITIRILTPRNGEVIESPANVPQAMATRDALAKAIYNNLFDWIVERVNKSLTARAETSNSIGILDIYGFEIFEQNSFEQLCINYVNEKLQQIFIQLTLKTEQEEYAREQIKWTPIKYFDNKIVCDLIEAIRPPGVFSAMKDATKTAHADPAACDRTFMQAISGMSNPHLTPRQGNFIIKHYAGDVSYTVEGITDKNKDQLLKGLLNLFGQSRNHFIHELFPHQVDQDNRKQPPSAGDKIKASANDLVTTLMKATPSYIRTIKPNENKSPTEYNEKNVLHQVKYLGLQENVRIRRAGFAYRQTFDKFVERFYLLSPKTSYAGDYIWTGDSKTGAMQILKDTNIPVEEYQMGVTKAFIKAPETLFALEHMRS",
    "response#2": "VGVSDLTLISKVSNEAINENLKKRFDNREIYTYIGHVLVSVNPFRDLGIYTDAVLESYKGKNRLEMPPHVFAVAESAYYNMNAYKDNQCVIISGESGAGKTEAAKRIMQYIANVSGGSNSSIQETKEMVLATNPLLESFGNAKTLRNNNSSRFGKYLQLQFNAQGEPVGADITNYLLEKTRVVTQIKDERNFHIFYQFTKGASQAYRESYGIQQPSQYLYTSKAGCFDVDGIDDLAEYQDTLQAMKVIGLSQAEQDEIFRMLAAILWTGNIQFREGDDGYATVVDQSVVDFLAYLLDVDAAHVIQAITIRILTPRNGEVIESPANVPQAMATRDALAKAIYNNLFDWIVERVNKSLTARAETSNSIGILDIYGFEIFEQNSFEQLCINYVNEKLQQIFIQLTLKTEQEEYAREQIKWTPIKYFDNKIVCDLIEAIRPPGVFSAMKDATKTAHADPAACDRTFMQAISGMSNPHLTPRQGNFIIKHYAGDVSYTVEGITDKNKDQLLKGLLNLFGQSRNHFIHELFPHQVDQDNRKQPPSAGDKIKASANDLVTTLMKATPSYIRTIKPNENKSPTEYNEKNVLHQVKYLGLQENVRIRRAGFAYRQTFDKFVERFYLLSPKTSYAGDYIWTGDSKTGAMQILKDTNIPVEEYQMGVTKAFIKAPETLFALEHMRSDLTLISKVSNEAINENLKKRFDNREIYTYIGHVLVSVNPFRDLGIYTDAVLESYKGKNRLEMPPHVFAVAESAYYNMNAYKDNQCVIISGESGAGKTEAAKRIMQYIANVSGGSNSSIQETKEMVLATNPLLESFGNAKTLRNNNSSRFGKYLQLQFNAQGEPVGADITNYLLEKTRVVTQIKDERNFHIFYQFTKGASQAYRESYGIQQPSQYLYTSKAGCFDVDGIDDLAEYQDTLQAMKVIGLSQAEQDEIFRMLAAILWTGNIQFREGDDGYATVVDQSVVDFLAYLLDVDAAHVIQAITIRILTPRNGEVIESPANVPQAMATRDALAKAIYNNLFDWIVERVNKSLTARAETSNSIGILDIYGFEIFEQNSFEQLCINYVNEKLQQIFIQLTLKTEQEEYAREQIKWTPIKYFDNKIVCDLIEAIRPPGVFSAMKDATKTAHADPAACDRTFMQAISGMSNPHLTPRQGNFIIKHYAGDVSYTVEGITDKNKDQLLKGLLNLFGQSRNHFIHELFPHQVDQDNRKQPPSAGDKIKASANDLVTTLMKATPSYIRTIKPNENKSPTEYNEKNVLHQVKYLGLQENVRIRRAGFAYRQTFDKFVERFYLLSPKTSYAGDYIWTGDSKTGAMQILKDTNIPVEEYQMGVTKAFI",
    "response#3": "VGVSDLTLISKVSNEAINENLKKRFDNREIYTYIGHVLVSVNPFRDLGIYTDAVLESYKGKNRLEMPPHVFAVAESAYYNMNAYKDNQCVIISGESGAGKTEAAKRIMQYIANVSGGSNSSIQETKEMVLATNPLLESFGNAKTLRNNNSSRFGKYLQLQFNAQGEPVGADITNYLLEKTRVVTQIKDERNFHIFYQFTKGASQAYRESYGIQQPSQYLYTSKAGCFDVDGIDDLAEYQDTLQAMKVIGLSQAEQDEIFRMLAAILWTGNIQFREGDDGYATVVDQSVVDFLAYLLDVDAAHVIQAITIRILTPRNGEVIESPANVPQAMATRDALAKAIYNNLFDWIVERVNKSLTARAETSNSIGILDIYGFEIFEQNSFEQLCINYVNEKLQQIFIQLTLKTEQEEYAREQIKWTPIKYFDNKIVCDLIEAIRPPGVFSAMKDATKTAHADPAACDRTFMQAISGMSNPHLTPRQGNFIIKHYAGDVSYTVEGITDKNKDQLLKGLLNLFGQSRNHFIHELFPHQVDQDNRKQPPSAGDKIKASANDLVTTLMKATPSYIRTIKPNENKSPTEYNEKNVLHQVKYLGLQENVRIRRAGFAYRQTFDKFVERFYLLSPKTSYAGDYIWTGDSKTGAMQILKDTNIPVEEYQMGVTKAFIKAPETLFALEHMRS"
  },
  {
    "reference": "MRPEQGKVVVVGSLNMDIVVRSSRFPASGETMFGQEVHFIPGGKGGNQAVACARLGHKAVMLGAVGDDGFGQSLLESLKANGVRTGHMKKTNAAATGIASITLTPADNTILVVPGANETLTAEDINAWSSVISEASILLVQLEIPMEAVAAAVDIADRHGIPVILNPAPARKIAADILRKVRYITPNQSELLELTGIALSDEGLERAIDALLERGPEFVVATLGSKGAAWKQRGGHLQRIDAHRLEVTDTTGAGDAFNAGLACTLSRGELIGNGVRFAVAVSALAVTKFGAQDGMPMKEEVERFMRSFNEGATR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that is capable of catalyzing reactions within the D-tagatose 6-phosphate degradation pathway with high efficiency and specificity.2. The protein design must be able to perform tagatose-6-phosphate kinase activity in order to contribute to lactose metabolic process.3. The protein must exhibit the following characteristics: The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway..4. The presence of Carbohydrate kinase PfkB domains is critical for the interaction of the designed protein with its intended targets.5. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.6. The protein should have a high affinity for ATP. The designed protein sequence is ",
    "response#1": "MEKLCVIGAVNMDIILEVDASPLQGETTVANSTHKALGGKGSNAAIATKRLGLDTHFYGCVGNDENGNELRENLENEGIDISSMTIFDEVSTGTAYIILEKDGNNRIIAAPGGNQAITETEIRENCAPLIEEADLVFMNLEISQEAAKAVLEVCKEKDKKIVVDAGPAKGWSAEDFKDIYMISPNEQELSDLMHYEIKTRDEVIEAGKKLLDLGIEHVIVKLGEKGSIYIGQDDIYDQKKYDVNVKDTTAAGDIYMAGLCKGLLEEQTIPESMDLASKVAAISVTRLGASPSAPDNLEKLLGE",
    "response#2": "MADITVIGSNMIDLMTYIDRMPEEGETISAPSFSMGFGGKGSNQAIAASRLGSDVAMITAVGADEFGQQQLDNYQENGIKTDMIIHGKANSGVAPIFVEPTSGNRIIIVKGANDELTPDALDQFKSQIQSSKLIVLQQEIPLETNYRAIEIARAANVPVLLNPAPAVEGINREFVNQVQFYAPNETELSTITGMPVESIDEIKAAAHKLIDEGVENLIVTIGSRGVLWVCKDDFKLIDAHKVDAVDTTGAGDSFIGAFAHYYVAGESVSEALQHANDYAAVTVTRKGTQKSYPTR",
    "response#3": "MEKLCVIGAVNMDIILEVDASPLQGETTVANSTHKALGGKGSNAAIATKRLGLDTHFYGCVGNDENGNELRENLENEGIDISSMTIFDEVSTGTAYIILEKDGNNRIIAAPGGNQAITETEIRENCAPLIEEADLVFMNLEISQEAAKAVLEVCKEKDKKIVVDAGPAKGWSAEDFKDIYMISPNEQELSDLMHYEIKTRDEVIEAGKKLLDLGIEHVIVKLGEKGSIYIGQDDIYDQKKYDVNVKDTTAAGDIYMAGLCKGLLEEQTIPESMDLASKVAAISVTRLGASPSAPS"
  },
  {
    "reference": "MATRTLESRFERMSVNDENEPVDGGKLYSKTQSLVKTSQLSQSSSRPNLVKVALQSQSTNAVASVTLPSQTVQRHAKNPASPTRKPLPSTSSRTSDEVAEIERKSAVSIILEPSTPKVFHLGMFEIGRPLGKGKFGRVYLARERTSGFICALKVLHKNELQQGRVEKQLYGHFHDSKRVFLILEFAGKGELYKHLRKESRFPEWKSAQYIAQMASALRYLHRKHVIHRDIKPENILMGIHGEIKISDFGWSVHAPNNRRNTMCGTLDYLPPEMIKPGSSDNYYNEKVDLWSLGVLTYEFLVGEAPFEDTPVMTQRRIARADMTVPSFVSPEAKDLIKKLLVLDPEKRIPLEQVQTHPWIVKHCVKGERAANRERSS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be able to modulate positive regulation of mitotic chromosome condensation, positive regulation of mitotic sister chromatid arm separation in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "ASLMQHFLPCILDSCFSKNLRYSMLGIEILKCIIHQGLVNPRMCFSTIIALESNAIKETREVAILLHTELHRRHESLIDGLYAQSADLIFSLQKTEEYQTFKLGEFSPFQSAYTIVSADKSSKSRKKLIMQILKPLKLDGIDLPSFTEEKVSFVSFCCVCLAGIPYVSIEEPLMIISTVDSC",
    "response#2": "LLVVPLLGFVGFWHQEVQRVGFCGVPAEPYPSSLYYLQPGVLRSSIESAYSFAHSLGIEASCQPCPENAECGFNRQLFCKEGLKASFPLLADFGLKPYPRCIPNTVKVNKVEEMVQAFMSIIGKWYYKAPKEFATFESAKNLNGKSFVDNFKDRYYMYKQDIDNVVGLKDFKVYLKTTLNRLYNSKLTRKVLYYLFSPLFTLELWKLRVRGALSKFPTNCLRSVYSHTVSLMKYLTSAVISCWRIYLLIGILAAITGTVVWRIRVYAKKHVVKHGVSVCVSHCIAKLQKTKLKSLTDFSVNPRVEVVQLRSDCFVSGVADDKGLFELVHLPLSIQLEIWEKVVSVLEGMVSVKVWDSERLAKNRAWEWIR",
    "response#3": "ASLMQHFLPCILDSCFSKNLRYSMLGIEILKCIIHQGLVNPRMCFSTIIALESNAIKETREVAILLHTELHRRHESLIDGLYAQSADLIFSLQKTEEYQTFKLGEFSPFQSAYTIVSADKSSKSRKKLIMQILKPLKLDGIDLPSFTEEKVSFVSFCCVCLAGIPYVSIEEPLMIISTVDSC"
  },
  {
    "reference": "MAARLPRRCSRPSPSGPQVTWGFSRIRSQKSPSPQRAAADSMKLSALLSGLDFRQSAGAADPECAGLAYDSRRVTPGMVFFALPGEKTDGHDHAAEAAAHGAVAVVSRRDCGLPPDVTSVFVDNPRQTLARAAATFYRQPANDLKVIGITGTNGKTTVAFLVRGILNAVGLRTGMIGTVEYDVGSRTTPASRTTPESLELQQLLTQMRDANCAACVMEVSSHALEQNRVEAVDFDLGVFTNLTQDHLDYHGDMETYFASKQKLFEMLGDPLAAVVNLDDDYGSRLMIPGRLSFGFAQDAVVRAVQCELHGDHTRLVAKTHEGEVELLLSCIGRHNVSNALAAFAVARSMGVEPEAIAEAFSALPPPPGRLEAIRCGQSFGVFVDYAHTDDALHHVLTTLRELTRGKLRVMFGCGGNRDAGKRRKMGAAAAALADDIIITNDNPRHEDPVTIAEQVAVGCREARPNDFEIELDRQRAIDEMLRRARPEDIVLLAGKGHETYQEIGDVTQPFDDREQARSVLRAMGYED",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be involved in cell division, cell cycle, regulation of cell shape, cell wall organization by localizing to cytoplasm and exhibiting tetrahydrofolylpolyglutamate synthase activity, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, ATP binding, magnesium ion binding.2. Ideal protein should have a binding affinity for key enzymes in the Cell wall biogenesis, peptidoglycan biosynthesis pathway to enhance the overall efficiency of the pathway.3. The protein should have a high affinity for meso-2,6-diaminoheptanedioate, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP.4. The protein should have a low Km value for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.5. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "MKLNKLLEKVAPELLYGEPDVAEISGIQSDSRKVEKGFLFVAVRGTTVDGHDYIQSAIGKGAAAIVCEEIPALSDEDIQVSESKPVFIRVKDSADALGKSLSAWYGHPARSLFMIGVTGTNGKTSTTYLIHGLLQKAGFKCGLLGTIEFVNGDKKIAARNTTPNAFELQRHFAEMVDAGCQAAVMEVSSHALVLGRVADCRYDVAVFTNLTQDHLDFHGDIGAYFAAKKRLFTDYLNRKSPGKAVINIDDPYGRRLIEQADIPVWSFGQDKDADVHARNIRFSSLGSTFEVVTPRGSLPVTTRLVGRFNVYNVLAAIGAALSLGVPVNTISAGLAQAPGVPGRMEVVSDEPRVIIDFAHTPDGMEKVLNALKNHKLIVVFGAGGDRDRLKRPLMAKMAEHFAYKIIVTSDNPRSEKPEDIIDDIMVGFENKERVLVEVDRKKAIEKALSLAKDGEYVVILGKGHEDYQIIGDEKVHFDDREEAAKALRL",
    "response#2": "MKIRTHSGQVKPGDVFVALPGTQTDGSRYIPQAVQNRAAWVVYPEHIRPLRGQSRVRFVPCRDVVSALGELAAARFYGRPSEQLQVVGITGTNGKTTSTHIVESILNTAGIKTGLIGTIYYRLGEKIYEYEGRTTPDPIKWHSTLAEMRKEEAKVVVCEVSSHALDQKRVWGTVFHTVAFTNLSQDHLDYHQTMEDYFLAKARLFTEYEYQHALINADDPWGKRLISMAKKVKTYGKEGDLKMLDFSTGLEGSRLKVEYEGKEYTFYSNLRGGFQAYNLSLGIASALAVGTDPQVIREGIEQCKGVPGRMEIVTNSPYVLVDYAHTPDALENVLVTLNQLKKGHLWVVFGCGGDRDKTKRPIMGKAASLHADKVVITSDNPRSENPESIVNEIADGIANSVDVVKLVNREDAIAHALNKASKEDIILIAGKGHEDYQIIGREKHPFDDREVARAALAARKA",
    "response#3": "MKLRDLLKGVNFVSTAGDPDVAVTGITNRSGEVRPGDLYAAVPGTRTHGAKFAAEAQAAGAVCVLTDAEGSDAVAASHVTLPIIVVPDVKAVLGEASARFYGDPSKKLRVVGITGTNGKTTSTHIVESILNTAGIKTGLIGTIYYRLGEKIYEYEGRTTPDPIKWHSTLAEMRKEEAKVVVCEVSSHALDQKRVWGTVFHTVAFTNLSQDHLDYHQTMEDYFLAKARLFTEYEYQHALINADDPWGKRLISMAKKVKTYGKEGDLKMLDFSTGLEGSRLKVEYEGKEYTFYSNLRGGFQAYNLSLGIAAAALGTGGRFELLDEGQEFAVLVDYAHTPDALDNVLKTARSLTNGKLICVFGCGGDRDRGKRPEMGLVAGKWADLLWVTSDNPRSEDPNSIIGHIVKAVPANVVCATDXNRASAIESALHGASFGDCVVIAGKGHEAYQILKDGKIPFDDREVARRVLEERKE"
  },
  {
    "reference": "MAKGSGMIHPNMATMFGLIASDVVMSADCLQSILAGSVRHSFNCVTVDGDTSTNDACALVATQTAGHRLIDDPKSPDAQQFASALSDLCDDLAEMLARDGEGATKFVRVICEEAVSDSDAHTIANTVALSPLVKTALAASDPNWGRIVAAVGRAPVSEIAIERVNVWINDVQIVQNGGRHPQYTEEAGQAAMNPPEIDIRIAMGAGSGYCRVMTCDLTKEYVRINAEYRT",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The substrate binding site should be stable and able to withstand changes in environmental conditions.2. Ideal protein should have a binding affinity for key enzymes in the Amino-acid biosynthesis, L-arginine biosynthesis, N(2)-acetyl- L-ornithine from L-glutamate: step 1/4 pathway to enhance the overall efficiency of the pathway.3. The protein must exhibit the following characteristics: Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate..4. A protein that can perform methione N-acyltransferase activity, glutamate N-acetyltransferase activity, acetyl-CoA:L-glutamate N-acetyltransferase activity in cytoplasm to support arginine biosynthetic process.5. The protein with the reaction acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate, the Nucleophile must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MANLSAXLPXLVDXLXXLGXLPGLAXLAXHXPGXGXDXAXACTPXXHXDXPXAXT",
    "response#2": "MTSAMNINLPGGRTLTATLGIGRPLPLPEGVSVAAPHVLAAWPDAGVSGPRVGLTHPALPEGFTAEARAAAEAAGAGLGLFAEVEAGEGLKTSDRWLDALAALDPQPAALVALGGGTVIDLAKMAAYRLGADPAVPPRLLAVPTTYAGSEATRVAATRAGDKHTMSGPEARAAVVLADTAVTLAAPRSLTLSGLRALAHALDNLWSVRACRESYALSAQALALARDGLPALAADPGDGDARAWLLRGSWLCGVAMALSGTTAPYALSYPLTQRFDVPHGHTCALTLAAPLAWRWCAPAVERAFARVGRAVPGGLDAGLEARAALEALSQDIGVPTSLRDLGVPAAAFEDAVEAALAAPSLINNRPVALDAAGAAAILRAAF",
    "response#3": "MTIAAALEWLRAHIDDTARLVVLGMGDVVLDAWARGATDRTSPEGPVPVVRGGERVPVPNLSAAFALGDCAAALATASDAPVVSIASAAGDWPALAVGIAASTGPIAVLVGTIDAVFPDGPRASGVSTSEPDLLALASTLGAAGARSVVLEVSSHALGQHRTAGLALDVAVFTNLTQDHLDHHASFDEYRAAKARIFGDLDPRGVAVVNADDPAWPSVARAARHRVIGTREAADLTVEVVLPELGTMRLRAAVGGVHNVENVLAAVTAATALGIELDTIAAGLAAATPVP"
  },
  {
    "reference": "MGGKWSKRSMGGWSAVRERMRRAKPAEERGKRAEPAADGVGAASRDLERHGAITSSNTAATNADCAWLEAQQDEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEMGGLEGMIHSQQRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPDKVEEASAGENNCLLHPMHQHGMDDPEREVLEWRFDSRLAFHHMARELHPEYYKNC",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for stability and expression in membrane where it will participate in suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I.2. For general function, the protein need meet that Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis The designed protein sequence is ",
    "response#1": "EAFGIL",
    "response#2": "GPLCSS",
    "response#3": "GNRVHW"
  },
  {
    "reference": "MSFKKIAEMMRQAERETKKKTLAFEQQAFEYNYKNGAIKITILGDLTLKSINIDPVLIDASDKVILEEMIIEATNEAVSDVKTKYDNLVEKTMPKVPGLF",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must be able to DNA binding. The designed protein sequence is ",
    "response#1": "LGNKINDEITKIAETQETIWGFVGKTMDLIEARTTRPTTKAAYNLLLQATFMNCCRADDLKNTDIKTFEVIPDKHLGRMLRAFVPETKTGTRFVYFFPCKGRCDPLLALDSYLQWTDPIPKTRTTDEDARYDYQLLRNSLLGSYDGFISKQSDESIFKIPNGPKAHLGRHVTASYLSNNEMDKEATLYGNWSAAREEGVSRVAKARYMHTIEKSPPSYLFAFLSGFYNITAERACELVDPNSNPCEQDKNIPMISDIETLMARYGKNAEIIPMDVLVFLSSYARFR",
    "response#2": "LGNKINDEITKIAETQETIWGFVGKTMDLIEARTTRPTTKAAYNLLLQATFMNCCRADDLKNTDIKTFEVIPDKHLGRMLRAFVPETKTGTRFVYFFPCKGRCDPLLALDSYLQWTDPIPKTRTTDEDARYDYQLLRNSLLGSYDGFISKQSDESIFKIPNGPKAHLGRHVTASYLSNNEMDKEATLYGNWSAAREEGVSRVAKARYMHTIEKSPPSYLFAFLSGFYNITAERACELVDPNSNPCEQDKNIPMISDIETLMARYGKNAEIIPMDVLVFLSSYARFR",
    "response#3": "LGNKINDEITKIAETQETIWGFVGKTMDLIEARTTRPTTKAAYNLLLQATFMNCCRADDLKNTDIKTFEVIPDKHLGRMLRAFVPETKTGTRFVYFFPCKGRCDPLLALDSYLQWTDPIPKTRTTDEDARYDYQLLRNSLLGSYDGFISKQSDESIFKIPNGPKAHLGRHVTASYLSNNEMDKEATLYGNWSAAREEGVSRVAKARYMHTIEKSPPSYLFAFLSGFYNITAERACELVDPNSNPCEQDKNIPMISDIETLMARYGKNAEIIPMDVLVFLSSYARFA"
  },
  {
    "reference": "MKLRELLAAVPGVTQPSSHAALESEVKGLTTNSLACQPGDLFIGMPGTRVDGGEFWPSAIASGAVAALVSSTAAQKRPHTEAEDACVIPASDMVQACAQVAAAFYDYPAQKLQLVGVTGTNGKTTTTHLIEFLLNEANLSTALFGTLYARWPGYQKTAAHTTPFAIDLQQNLAAAVAAGCEYGVMEVSSHALAQGRVLGCGFQVAVFTNLTQDHLDYHRDLEDYFAAKALLFSPGYLKGRAIVNQDDAYGRRLIDQLPAEQIWSYSTQDSNADLWTSDLTYEANGVSGTLHTPKGAIAFRSPLVGQFNLANLLAAVGTALHLGLELETIVAALPQFVGVPGRMERVQINDEQDISVIVDYAHTPDSLENLLKAARPFIKERMICVFGCGGDRDRTKRPQMGKIAAELADLAIVTSDNPRTEDPERILQDVLAGIPTTVKPLVICDRAEAIRTAILEAKPGDGVLIAGKGHEDYQILGTEKIHFDDREQARAALELR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Incorporating a Meso-diaminopimelate recognition motif motif is critical for the successful design of the protein.2. The protein should be designed to catalyze the following reaction ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.3. The protein should be able to bind UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate, ATP ligand in a variety of conditions4. The ideal protein would be one that plays a critical role in cell wall organization, cell cycle, peptidoglycan biosynthetic process through magnesium ion binding, ATP binding within cytoplasm.5. The presence of Mur ligase N-terminal catalytic, Mur ligase central domains is critical for the interaction of the designed protein with its intended targets.6. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan. The designed protein sequence is ",
    "response#1": "MKLKDILYKVAIEAVKGSTDIEVKGLSTNSHACQPGDLFIGMQGTRVDGGEFWESAITSGAIAAIISPQAAAKCPSQGKACIASVTAITQDSREVVPGSLFIAVKGIRADGHEFVSQAIENGAAALVLQRGRVPPEGLEVPVFEVDDTTAACHFLARRFYGEPSSKLKVVGVTGTNGKTTTTLLIEYLLTYVGMKAGLIGTIETIIGDEVIPAKNTTPESYDIQRYFAKMVDAGCQCVVMEVSSQGLMLHRTQGFLFDIGIFTNLEPDHIGENEHKDFEDYKRCKGLLFKQCKIGICNGDDQHVEEILAGHTCEVETFGFSEKADLRAANVELTGPRGIGFDVSYDGIIEKMNITIPGKFSVYNSLAAIAVCRHFGVDEKVALEGLQKAKIQGRWERIPSGLGFEVVVDYAHTPDALERVLKIGKRLCPGKLITVFGCGGDRDRSKRPLMGEVAARWSDYLVLTSDNPRNEDPLDIIAEIEEGVRRAFPGSSNYVKIEDRRKAIQEAIGAAREGDLVIIAGKGHEDYQEIKGVKHHMDERELVREILEEKE",
    "response#2": "MKLKDLIQGAEVVDVIGAADVEVSGMSLSTQRIQAGDLYAALPGSRLHGATFAADALAAGAVAVLTDEEGARMLAPGCAALVVERPRHVLGRLAARIYGQPSGRLALVGVTGTNGKSTTAFMAQAILRAAGSPCGLLGTVQYDLCRRCVTADMTTPGPLDLAALLRECADAGAAAAVLEVSSHALEQRRADGLRFSAAAFTNLTQDHLDYHRTLEAYAAAKARLFEQLDASGVAVVNADDPRHERMLQGCRARVLRYSLERPAEIMAAITRDTIEGTLYRLRIDGRDLVLHNALVGRHNVYNAMAAAGLALGVSGTPFEALAAGLANVRVPGRLERAVITRLGAPVYVDYAHTPDAIEAAIAALKPHASGKLIVLIGAGGDRDPGKRAPMGAIAAGLADRVIVTDDNPRTEDPAAIRADVMRGAVGATEIGDRREAIAAAIAAAGPDDIVLLTGKGHETGQEFRGVVHPFDDRAVVAEALEARGA",
    "response#3": "MQLGTLLGALGLDLSAAGDPAVTGFAIDHRKVAPGTVFGAFPGMRVNGEDFIAAAVAGGAIAVVARPEARVEGAVHIADDHPRAAMPLASARLVGGEPRRAFARLAAAFYGAQPETVVAVTGTNGKSTTAFMAQAILRAAGSPCGLLGTVQYDLCRRCVTADMTTPGPLDLAALLRECADAGAAAAVLEVSSHALEQRRADGLRFSAAAFTNLTQDHLDYHRTLEAYAAAKARLFEQLDASGVAVVNADDPRHERMLQGCRARVLRYSLERPAEIMAAITRDTIEGTLYRLRIDGRDLVLHNALVGRHNVYNAMAAAGLAIACGADPDALFAALSALAGAPGRLELVGRYRDAPIFVDYAHKPDALDKVLRSLRPLIKGRLIAVFGCGGDRDQGKRPIMGELAARLADVVIITDDNPRSEAPAAIRGAIREGASRVAGANLREIGDRGTAIVEAIALLAPGDALLIAGKGHETYQDLAGRRRAFDDRAIAAEALAARG"
  },
  {
    "reference": "MASLREQIITELGVQPVIEPKTEVRRRVDFLKDYLRSTPAQGFVLGISGGQDSTLTGRLCQLAAEEVRAEGGEATFVAVRLPYGVQADEHDAAVAMEFIGPDRAVTVNVKPGVDATAGAVAEGLGLDALRDFVRGNIKARERMIIQYAIAGQENLLVVGTDHAAEAVTGFFTKYGDGGVDLTPLTGLTKRQGAALLQELGAPPSTWSKVPTADLEDDRPALPDEEALGLRYSEIDDYLEGKEVTEAVAARVEQLYTATRHKRTVPVSPLDSWWR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be involved in NAD biosynthetic process by localizing to cytoplasm and exhibiting metal ion binding, NAD+ synthase (glutamine-hydrolyzing) activity, NAD+ synthase activity.2. The deamido-NAD(+), Mg(2+), ATP binding site should be stable and able to withstand changes in environmental conditions.3. The protein design should take into account the physiological conditions of the Cofactor biosynthesis, NAD(+) from deamido-NAD(+) (ammonia route): step 1/1, NAD(+) biosynthesis pathway.4. The protein should have a fast turnover rate for ATP + deamido-NAD(+) + NH4(+) = AMP + diphosphate + H(+) +   NAD(+).5. For general function, the protein need meet that Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD; Uses ammonia as a nitrogen source. The designed protein sequence is ",
    "response#1": "MSISRRVNFIKARLIAAHATSLVLGISGGVDSSVCGRLCQLAVNELNQEQSTTDYKFVAVRLPYGVQADENEAQLAVDFIQPSSRMTVNIKPATDALHEQTMAAIVGNGESLPEQEKIDFIKGNVKARQRMIAQYEIAAFCQGLVVGTDHSAENITGFYTKFGDGACDLAPLFGLSKRQVRALGSTLGASSVLVNKAPTADLESDRPGLTDEEALGLSYEQIDDFLEGKPVTQQVEQTLSAIYQRTQHKRQRLALTDNR",
    "response#2": "MLVDRIAQAMRDQVRNKMRRRGLVIGLSGGVDSSVCAALAAYALGAQNVFAIFMPENDSDPESLSLGQEVAKAFSLEGAIEDIGSALAAGRIAGPNAMMVGIRTRVDFLKSYLKKHTFSTGYVLGMSGGQDSTLLSKLTQIAVNELNEENSTNTYQFIGMKLPYGVQKDADDVDDAIKFVEPSKVLTVNIKESVDASVKALDGAGVEISDFLKGNEKARERMKAQYSVAGAFNCFVLGTDHAAEAVTGFYTKHGDGACDLAPLFGLNKRQGKQMLVALNCPEHLYNKKPTADLEDDRPALPDEEALGVTYEQIDEFLEGKEVAEDSKRTIEGHYLKTMHKRKIQELAAWNKPYYTGVEL",
    "response#3": "MPISRRVNFIKARLIAAHATSLVLGISGGVDSSVCGRLCQLAVNELNQEQSTTDYKFVAVRLPYGVQADENEAQLAVDFIQPSSRMTVNIKPATDALHEQTMAAIVGNGESLPEQEKIDFIKGNVKARQRMIAQYEIAAFCQGLVVGTDHSAENITGFYTKFGDGACDLAPLFGLSKRQVRALGSTLGASSVLVNKAPTADLESDRPGLTDEEALGLSYEQIDDFLEGKPVTQQVEQTLSAIYQRTQHKRQPHA"
  },
  {
    "reference": "MRHYEIVFIVHPDQSEQVPAMIDRYKSIVTARNGQIHRLEDWGRRQLAYPIQKVHKAHYVLMNIECDGEALGELEHAFKFNDAVLRHLTIKMKKAVTTPSPMMKEEKSRSLTPAAGDEGKPAEAAEA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that can perform structural constituent of ribosome in ribosome, ribonucleoprotein complex to support translation.2. The protein must exhibit the following characteristics: Binds together with S18 to 16S ribosomal RNA.. The designed protein sequence is ",
    "response#1": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTD",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTV",
    "response#3": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTL"
  },
  {
    "reference": "MLRWLIGGGREPQGLAEKSPLQTIGEEQTQNPYTELLVLKAHHDIVRFLVQLDDYRFASAGDDGIVVVWNAQTGEKLLELNGHTQKITAIITFPSLESCEEKNQLILTASADRTVIVWDGDTTRQVQRISCFQSTVKCLTVLQRLDVWLSGGNDLCVWNRKLDLLCKTSHLSDTGISALVEIPKNCVVAAVGKELIIFRLVAPTEGSLEWDILEVKRLLDHQDNILSLINVNDLSFVTGSHVGELIIWDALDWTMQAYERNFWDPSPQLDTQQEIKLCQKSNDISIHHFTCDEENVFAAVGRGLYVYSLQMKRVIACQKTAHDSNVLHVARLPNRQLISCSEDGSVRIWELREKQQLAAEPVPTGFFNMWGFGRVSKQASQPVKKQQENATSCSLELIGDLIGHSSSVEMFLYFEDHGLVTCSADHLIILWKNGERESGLRSLRLFQKLEENGDLYLAV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Non-catalytic component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy. The designed protein sequence is ",
    "response#1": "VEPEDVANHLTAFHWELFRCVHELEFVDYVFHGERGRRETANLELLLQRCSEVTHWVATEVLLCEAPGKRAQLLKKFIKIAALCKQNQDLLSFYAVVMGLDNAAVSRLRLTWEKLPGKFKNLFRKFENLTDPCRNHKSYREVISKMKPPVIPFVPLILKDLTFLHEGSKTLVDGLVNIEKLHSVAEKVRTIRKYRSRPLYLDMEASPHHLQTKAYVRQFQVIDNQNLLFELSYR",
    "response#2": "VEPEDVANHLTAFHWELFRCVHELEFVDYVFHGERGRRETANLELLLQRCSEVTHWVATEVLLCEAPGKRAQLLKKFIKIAALCKQNQDLLSFYAVVMGLDNAAVSRLRLTWEKLPGKFKNLFRKFENLTDPCRNHKSYREVISKMKPPVIPFVPLILKDLTFLHEGSKTLVDGLVNIEKLHSVAEKVRTIRKYRSRPLYLDMEASPHHLQTKAYVRQFQVIDNQNLLFELSYA",
    "response#3": "EPEDVANHLTAFHWELFRCVHELEFVDYVFHGERGRRETANLELLLQRCSEVTHWVATEVLLCEAPGKRVQLLKKFIKIAAICKQNQDLLSFYAVVMGLDNAAVSRLRLTWEKLPGKFKNLFRKFEGLTDPCRNHKSYREVISKMKPPLIPFVPLILKDLTFLHEGSKTLVDGLVNIEKLHSVAEKVRTIRKYRSRPLCLDMEASPHHLQTKAYVRQFQVIDNQNHLFELSYKLEAC"
  },
  {
    "reference": "MAGRSGASDTELLQVCKIIKILYQSNPCPEPTGTRQARRNRRRRWRARQRQIREISERILTSCLGRPPEPVTLQLPPLERLTLNCSEDCGTSGEKGEGSPQISLESSTILGTGTKE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be involved in viral process by localizing to host cell nucleolus and exhibiting RNA binding, DNA-binding transcription factor activity.2. For general function, the protein need meet that Conversion from Ran-GTP to Ran-GDP mediates dissociation of the Rev/RRE/XPO1/RAN complex, so that Rev can return to the nucleus for a subsequent round of export; In the nucleus, the conversion from Ran-GDP to Ran-GTP dissociates Rev from KPNB1 and allows Rev's binding to the RRE in viral pre-mRNAs; This function is essential since most viral proteins are translated from unspliced or partially spliced pre-mRNAs which cannot exit the nucleus by the pathway used by fully processed cellular mRNAs; Beside KPNB1, also seems to interact with TNPO1/Transportin-1, RANBP5/IPO5 and IPO7/RANBP7 for nuclear import; Rev can then form a complex with XPO1/CRM1 and Ran-GTP, leading to nuclear export of the complex; KPNB1 binds to the GDP bound form of RAN (Ran- GDP) and targets Rev to the nucleus3. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "MEPVDPRLEPWKHPGSQPQTACTNCYCKKCCFHCQVCFTKKALGISYGRKKRRQRRRAPQDSQTHQASLPKQPASQPRGDPTGPKESKKKVETETETDPVD",
    "response#2": "MEPVDPNLEPWNHPGSQPETPCNKCYCKYCSYHCLVCFQKKGLGISYGRKKRRQRRSTPPSSEDHQNLVSKQPLPQTQGDSTGSEESKKKVESKTKTDPFD",
    "response#3": "MEPIDPNLEPWNHPGSQPKTPCTKCYCKKCSYHCQVCFLNKGLGISYGRKKRRQRRRAHQDSENHQNSLSEQPIPQPRGDPTGPKESKKKVESKTETDQFA"
  },
  {
    "reference": "MAGEFSRTSRIGDQLQKELASLIQFEVKDPRLGLVTVNEVRVAKDLGYADIYYTVLGKDDQPEVLAENQAALDSAKGFLRRRLAQEVKLRVMPHLRFHYDQSVVNGSRMSALIDEAIRDDETKNGNEDE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that May interact with the 5'-terminal helix region of 16S rRNA.2. The designed protein localizes to the cytoplasm. The designed protein sequence is ",
    "response#1": "XS",
    "response#2": "MPIGVPKVPFRSPG",
    "response#3": "MSTLLSL"
  },
  {
    "reference": "MSNLKQKDSKPEVAVTKSVKTYKKSIEYVNSDASDIEEDINRAEDEYASSSGFVNFLRDFKKRYGEYYSNNEIRRAAETRWNEMSFRHRCQYSAEPLDTFHVEPNSVSSLQRSSEGEHRMHSEISGCADTFFGAGGSNSCTPRKENKCSKPRVRKSCPKPRAKTSKQRRSCGKPKPKGARPRKACPRPRKKMECGKAKAKPRCLKPKSSKPKCSM",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Not known2. The designed protein should have a specific function within the chromatin.3. The designed protein's functionality will benefit from Basic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "LMELDKGLRSTKQGIQCEAVVRFPRLFEKYPFPILINSSFIKLADYFVSGSNLLRFWVLRVCQQSENHLDKILNIDSFVRCIFVVMHSNDPVARALLLRTLGAVSRVIPEKQQVHHAIRRALDSHDTVEVEAAIYASSCFAAQSSSFAISMCAKISDMIESLQVPVPMKLLLIPVLRHMHHEATTASLVSRLCMDLLPKYPAQSFVVAIIDTLTQLSSRTLVGVPGQLDVLLDFMQDLRTPVRIQVLRSFNELAGRQSVHAWPKPAIKALIDRFELCTNSKEQFLFLSILLKLSECPLTCQQLLREHRVALLRLCIQCISKLDDYTTATQAMAVLSVLVAFGLKKKGSGEQVDDILHMVNLHMEGLLLCTAKRSECTRDLRRVLTYGIRITKANAEFGTSFIGIVTNSLGDKGAYPPANAELMCEALAGLCEHFQLRKYAFSTAEDLIVDENAMDTDELPPPKINPMLARLPLILHKLNTIIDQENCDQQLRSVEILSSLVLQTTMGCYLPQKVVQCFEKCLGRLNCWTLYRIARTASRYGS",
    "response#2": "LMELDKGLRSTKQGIQCEAVVRFPRLFEKYPFPILINSSFIKLADYFVSGSNLLRFWVLRVCQQSENHLDKILNIDSFVRCIFVVMHSNDPVARALLLRTLGAVSRVIPEKQQVHHAIRRALDSHDTVEVEAAIYASSCFAAQSSSFAISMCAKISDMIESLQVPVPMKLLLIPVLRHMHHEATTASLVSRLCMDLLPKYPAQSFVVAIIDTLTQLSSRTLVGVPGQLDVLLDFMQDLRTPVRIQVLRSFNELAGRQSVHAWPKPAIKALIDRFELCTNSKEQFLFLSILLKLSECPLTCQQLLREHRVALLRLCIQCISKLDDYTTATQAMAVLSVLVAFGLKKKGSGEQVDDILHMVNLHMEGLLLCTAKRSECTRDLRRVLTYGIRITKANAEFGTSFIGIVTNSLGDKGAYPPANAELMCEALAGLCEHFQLRKYAFSTAEDLIVDENAMDTDELPPPKINPMLARLPLILHKLNTIIDQENCDQQLRSVEILSSLVLQTTMGCYLPQKVVQCFEKCLGRLNCWTLYRIARTASRYGR",
    "response#3": "HYVAAHIYTKVSQIVISDHMHYFLVALSQISQAECILNYGLEYAYMRDNYAPKVAPEPLIPLMKRLEMASNLYQQALASLRAGSSPQHPCTFQLEYLKIRAQFLQTLHLAVTVKNAQVIVPPPAIAGSLAQNSRDYLQKFGHVTNQLRKLVKALKACEETYARLYKSSFDADHVTLEFLEVAEFQCALFAHIIESC"
  },
  {
    "reference": "MRLLTTVAALRCYLTKRRTLRDATPKENELTVPEDILLDDTTSWYQTAVGLVPTMGGLHQGHLSLIERARSENFTVIVSIFVNPLQFGPNEDYQRYPRTLESDQKLCAQAGVDAIFAPTPEEMGLRPKNIAESHVTQVIPPSAMISALCGRYRVGHFQGVATIVTKLFNLVQPDRVYFGQKDGQQLAIIKRLVVDLNLPVEIVACPTVREASGLALSSRNQYLTATEKEQATVLSRGLRQAEAAFKAGTRHSSQLIALVWQEIATVSTIFVEYIELVDPTTLMFLETVEEEGMLAIAARLGSTRLIDNTILRDRQPIVAIDGPAGAGKSTVARQVAANLGLVYLDTGSMYRAVTWLVLNMGIAIDDDYAIAELVNQCKIELTPSQDLQSPVRVWINGTDVTQAIRTIEVTSLVSAIAAQTSVRAALVQQQQSWGKRGGLVAEGRDIGTHVFPDAEVKIFLTASVGERARRRQQDFKKQGQPEVSLEQLERDIAERDWKDSNRKVSPLQKAVDAVEIQTDGLSASEVTAKIVNYYQQRLSQW",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein with dCMP kinase activity, CMP kinase activity is needed to enhance pyrimidine nucleotide metabolic process in a specific context.2. The protein must exhibit the following characteristics: Catalyzes the transfer of a phosphate group from ATP to either CMP or dCMP to form CDP or dCDP and ADP, respectively..3. The Proton donor active site of the designed protein must be optimized for substrate binding and specificity.4. A protein that is capable of catalyzing reactions within the Cofactor biosynthesis pathway with high efficiency and specificity.5. The protein should be able to bind (R)-pantoate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "AQGSVQLPGSKSISNRVLLLAALSQGTTTVHDLLASDDTRVMLDALRQLGCGVEEDGAQARITGLGGRQPTSPATLFLGNAGTAMRPLTAALALLGGVFELSGIARMHERPIGDLVDALRQLGCHIDYLGNDGYPPLRIAQPDGVPALALSTPIRVRGDVSSQFLTALLMALPLAATHQDVVIEVVGELISKPYIHITLQLLERFGITVHHENWQRFTIAAGSSYRSPSSIHVEADASSASYFIAAGAIAAGGGMQNGIKILGVGLDSIQGDIRFVEAAQAMGAHITGGPNWLQIGRGAWPLKAIDLDCNHIPDAAMTLSVMALYAQGTTSLRNIASWRVKETDRIAAMATELRKLGATVEETADSIHITPPATPGDWRAASIHTYDDHRVAMCFSLAAFNPAGLPVRIQDPQCVAKTFPDYFEALFS",
    "response#2": "AVDGPAGAGKSTVTRLAAQALGLRYLDTGAMYRAVTWWCLENNLDLHDEPAIVEAIAHCRLHLESPDPHQPTRVWINDREVSQAIRSLEVTQRVSQIAALRGVRRLMVQQQRAIGAHGGIAAEGRDIGTHVFPEAGLKIFLTASPEERAQRRWQELQQQGHCDLSYEELLAQITARDTADQQRPYAPFRKAADAIEVCTDNLGIDEVVSKIVHLYAP",
    "response#3": "VIAVSGQPGSGKTTIAREIARVLGLPLVSSGLLFREMAARMGMDFIEFHKYAETNPDIDKKVDSLAIERAKAGDVVLEGHLTAWIVRPYADVCIYLKASLETRARRVALRDGKSLQDALREVAEREELNRRRYLSIYGIDINDLSIFDLVLDTSELEVSVTLNR"
  },
  {
    "reference": "MAMSMKKIGAIAVGGAMVASALATGALAAEKVGDVDAFAANAVTNGNVNVDIVVGSNAAAKDVVSAANIAAKVGSLMFKEGSATDGNAKVTVSASSKSDNIKNIWDAGNAFAATNEVFLFAAADDDYTGTAAGTGVPNYLLGKTFMDYTVPPAAQVIDLEDLQTLAAVSDADPSGWDIFGTNKDFEAAEAVAAVIRTQQPAAALFNTGADLQVSEDEAFYMSLAFEEDKYGSALADYARLFPGMRIPFLGQEMVVVKLDTDDDLVILGKEVYDGVVKQRGDNYDLGNGYAVKVNNVLTQGAAYKADVQLLKDGKVVASKFDTIDGNGNQITIVYKDIGIVVNDAWEDIGGNYGFAEMVITKDVVTLELGEEYINDWEAYAVRATAAGLIESDSATSTAAVPVVGIALKYVGDDVDKIRKDKTFSIANYAKLDFDEDSGDMAVQFLMDDAKDVTLSIGQKVSVLNAEIKLNELMADAKQSVPMTAPIAKLDAEASLSAANKDLILVGGPVVNTLAKELADKGLIAIDNASPATLAVAKGAANGKDVLVVAGGDRDKTAAAATALIAML",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must contain a signal peptide for proper functionality.2. For general function, the protein need meet that S-layer protein3. The protein should have a specific function in cell wall organization and should be localized to S-layer in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "WLSLGLPTPDYAVLASEDDCREAAQRLGFPLIVKPAHEGSSIGMAKVGGLDELIAAWREAARYDSQVLVEQWISGPEFTVATLRGQVLPAIRLGTPHTFYDYDAKYLASDTRYQVPCGLDEAKERELKELTARACDALGIQGWGRADVMQDAEGRFWLLEVNTAPGMTCL",
    "response#2": "WLSLGLPTPDYAVLASEDDCREAAQRLGFPLIVKPAHEGSSIGMAKVGGLDELIAAWREAARYDSQVLVEQWISGPEFTVATLRGQVLPAIRLGTPHTFYDYDAKYLASDTRYQVPCGLDEAKERELKELTARACDALGIQGWGRADVMQDAEGRFWLLEVNTAPGMTC",
    "response#3": "SWDATNFAGFFYDVNDDVSTESLTVTGLNGGNVIPEGGLVYETTIQMVDYEYSKPSVGWDQFPVLGFFAEEYIPINADKADKLAKLVLDSDDKYTIRTGEQLDLGEGYAIEAKQVDVDGEKVWLEFTKDGEFVDDEIISVVSGSDNTWEVELDDIQDEDDVVVLRVHVNQVFQGAVDSIAQIEGIWLIDYANAMKIESDDEFGDLDNVKINGATLTITNEDTFTLTRDDEVEIGQGMFFKVADTAASDLRYYPFVG"
  },
  {
    "reference": "MEAVILAAGKGTRMHSQLPKVLHTIGSKPMLAHVIDSAESAGARKIHIIVGNDGEQVKAQILNSASQVEVSWVIQKEQLGTAHAVLQAVPYFETKAQDDHILVLYGDVPLIKPDTLRQLLASTRAGAIGILTLITKNNKGLGRILRNESNDVIGIVEEKDASDLQKEIKEVNSGIMALPVARLESWLNRVENNNQQSEYYLTDIIGLAVSEKVAINSVVVINEIEAQGVNDKSQLALVERHFQMTNCTQLLEEGVILKDPSRTDIRGELSCGNGVEIDFNTLFEGSVMLGDNVKIGPNTIIKDSSIGNCTQILPGTIIEGATIGEETILGPNARIRPGTILGNRVKVGNFVETKKAILGEGTKANHLAYIGDAEIGKNCNIGAGTIFCNYDGANKHKTXLGNNVFVGSNSVLVAPVKLEDDSFVAAGSAINTNVPEGHLAVGRAKQRTISGWKRPTKNTKS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Mg(2+) is required for the proper folding and stability of the protein.2. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+), acetyl-CoA, UDP-N-acetyl-alpha-D-glucosamine binding.3. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc); The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain..4. Ideal protein should have a binding affinity for key enzymes in the UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway to enhance the overall efficiency of the pathway.5. The designed protein must have at least one MobA-like NTP transferase domain. The designed protein sequence is ",
    "response#1": "VVILAAGVGKRMRSDLPKVLHPLAGKPLLAHVIDAARRLAPHRIVVVYGHGGETVRKAMAGDDLHWALQEPQLGTGHAVMQALPHLDRDVPTLVLYGDVPLVPHDTLVGLTKAAGADGLGLLTIEAADPTGYGRVILGEDGGVERIVEQKDADAATRAIRLCNAGIIGAEVALLKDWLARVGNDNAQGEYYLTDLIAMARRDGRTCATIIAEDEAEVMGVNSRAQLAEAEAEHQRRIRDRLMLAGVSMLDPASTYIEAGVKIGKDTTIMPNTYLQGKVTVGEGCVLGPNTIIRDSSIENRCRILASVLEGAQLEDDVDIGPFAHLRKGAHLMKGVHMGNFGEVKDSTLGPGVKMGHFSYIGNAAIGADTNIGAGTITCNFDGEHKHPTEVGENVFIGSDTMLVAPLKIGAGARTGAGAVVTKDVPPDTLVVGVPARPKERAWQARRRAQAMADGATLIAPETVWFSHDTSLGRDVTIEPNVVFGPGVTVADGATIHAFSHLEGASVGASCSVGPYARLRPGAVMQAKSRVGNFVEMKKAVLGEGAKANHLTYLGDAEVGAGANIGAGTITCNYDGFFKYQTKIGAGAFIGSNSALVAPVTIGDGAIVGAGAVVTRDVSADALALVRPAPAERPTKP",
    "response#2": "VIVLAAGAGTRMVSDTPKVLHELGGRSMLAHCLSAAAGVGPRHLVVVLGHEHGRITGPVAELAQALDFPIDIALQDQQLGTGHAVLCGLAALPDDFTGTVVVTSGDIPLLDTATLQALIDTHRTAPAAVTVLTALLGDPPGEFGRLLTDATGDILRIIEQKDATDEERAIREINTGICCAQAADLKRWLPRLSADNAQGEYYLTDIVALAVADGEPVATAHPADVWQTEGANDRVQLAELERLYQRRQADALMAAGVTLFNPEATYIDADVMIGNDTIIEAGVQLKGQTVIGNGCYIGANSEIIASQIGDNVKITSSHIEKAKVAAGSDVGPFGRLRPGTELAENVHVGNFVEVKNSIVGQGTKIGHLSYIGDGDLGQDINVGCGTVFVNYDGKNKSRSTIGDNAFIGCNTNIISPVVIGEGAFTAAGSTITEDVPADALAIARATQRNIANWTLPQRPKKA",
    "response#3": "VILAAGQGTRMRSKLPKVLHPLAGQAMAAYPLQVANQVGSTRSVVVVGHGAEAVEEALRPFGADFVLQRQQLGTGHALLAAREALTGFNGTLLLLCGDVPLLREETVRALLEQHRRQQATVSVLTAHLPNPKGYGRVIRDAAGNVVQIVEERDASESQRLINEINSGIYCVDNALLRRHLPLLSSDNAQAEYYLTDIVGMAVDAGQHVVAVQPDDLVETEGANDRVQLALLGREYNRRLCEHWMREERAWQARRRAQAMADGATLIAPETVWFSHDTSLGRDVTIEPNVVFGPGVTVADGATIHAFSHLEGASVGASCSVGPYARLRPGAVMQAKSRVGNFVEMKKAVLGEGAKANHLTYLGDAEVGAGANIGAGTITCNYDGFFKYQTKIGAGAFIGSNSALVAPVTIGDGAIVGAGAVVTRDVSADALALVRPAPARWIRRWRKQD"
  },
  {
    "reference": "MVGEEMSLRKRLSKSSENAEGKEGDQRDRSEESLEPRSNGQINLKQLIAKKIQLTAEAEELKPFFMKEVGSHFDDFVTNLIEKSASLDNGGCAVTSFSVLEGENNHRAKDLRAPPEHGKIFVIRRSLLDELLEVDHIRTIYHMFIALLILFILSTLVVDYIDEGRLVLEFNLMSYAFGKLTVAMWTWCTMFLCTLTVPYFLFQRWARGYDRSSHPLVYSVFHCFLFVVFQVGVLGLGPLYVVLAYTLPPASRCIVICEQIRLIMKAHSFVRENVPRVLNSAKEKSRSVPVPTVNQYLYFLFAPTLIYRDNYPRTPTVRWGYVIMQFAQVFGCFFYVYYVFERLCTPLFRNIRQEPFSARVLVLCIFNSILPAALILFLSFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYANYYRTWNVVVHDWLYYYAYKDFLWFFTKKFKPAAMFAVFAVSAVVHEYALAVCLNFFYPVLFVLFMFFGMAFNFIVNDSRKRPIWNILMWTSLFAGVAVLLCFYSQEWYARQHCPLKNPTFLDYIRPRTWTCRYVF",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must be able to perform cholesterol O-acyltransferase activity under specific conditions. The designed protein sequence is ",
    "response#1": "VEVREYDPSKDLATVEDVERRCEVGPAGKLSLFTDLLGDPICRVRHSPSYLMLVAEIGPKEKKELVGMIRGCIKTVTCGITTKRLDLTHNKSQNDVVITKPLYTKLAYILGLRVSPTHRRQGIGFKLVKAMEDWFSQNGAEYSYFATENDNHASVNLFTGKCGYAEFRTPSILVNPVYAHRVR",
    "response#2": "RSSRWISSSVPQSNWDTDLGKFCSAEYHVNNTMSTVLFNEALQHVPKNAIVIEIAPHCLLQAVLRRAVDSSCVLSGLMKAKHENNVEYFCTALGKLFEAC",
    "response#3": "VKGFGGSLSDAAAMNILGLSQPAQDNLLRSYFSESGIEYNLVRLPMACSDFSTRPYSYDDVPHDYELTHFRLAEEDVKMKVGGRRPSAMSKRPLLLYASPWTSPAWMKSNGDVRGKGTLKGHAGDKYHKTWANYFIKFLDEYAKHNVTFWAVTAQNEPLAALLTPPEFPTIAFTAVQQRDFVVRDLGPALARSPHRTRLIILDDQRIHLPHWAKAVLGNATAASYVAGVGVHWYLDSIVPASCSLEATHKLFPHHFLLYTEACSGFLNLRFSVTLGCWERGDHYSHSILY"
  },
  {
    "reference": "MSHASALASQDVIAYLDAHEKKSMLRFITCGSVDDGKSTLIGRLLYDTKLLYEDQLAALEADSKRIGTQGEKIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADTPGHEQYTRNMATGASTADLAIILIDARQGVLKQTRRHSYICSLLGIRHVVVAINKMDLVDYSRAIFEKIETDYRAFAEKLGFSSVVCIPISALEGDNILTKSANTAWHKGPALLPYLETIEVEADASGAPFRLPVQWVNRPNLDFRGFSGTIAAGIVRPGDEIVVVHSGRRSSVTRIVTKDGDLPAAGPHQAVTLCLADEIDISRGDVLCAGKAPAEQTDQFAAHVIWMHDDEMIPGRQYFFKTANRIISGSISELKHKVNVNTLEHMSGKTLELNEVGFCNVTTSAPIAFDPYAEDRVTGSFVIIDKRTNNTVGAGLIDFSLRRAQNVHWQDLDVNKAARAKAKHQKPCVLWFTGLSGAGKSTVANLVEKRLCDLGRHTYTLDGDNVRHGLNKDLGFTDADRVENIRRVAETAKLMVDAGLIVLVSFISPFQSERRMAREMVEDGEFIEVYVSTPLAVCEKRDVKGLYAKARRGEIKNFTGIDSAYEAPERAEITLDTSKATAAEAATAIVEHLKKNGYLG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must be able to sulfate adenylyltransferase (ATP) activity, GTP binding.2. The designed protein should have a high specificity for GTP binding, with minimal off-target effects.3. Ideal protein should have a binding affinity for key enzymes in the hydrogen sulfide biosynthesis, sulfite from sulfate: step 2/3 pathway to enhance the overall efficiency of the pathway.4. The protein must exhibit the following characteristics: APS synthesis involves the formation of a high-energy phosphoric- sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.. The designed protein sequence is ",
    "response#1": "DMLRVLTCGSVDDGKSTLIGRLLFDSKMIFEDQMAAIEKDSKRFNTTDDSFDLALLVDGLQSEREQGITIDVAYRYFTTEQRKFIIADTPGHEQYTRNMATGASTCDLAIILIDARHGVQVQTRRHSFICSQLGIKHVIIAINKMDAIDYDQATYQKIKKEYREFAEDLSFSDVRFVPISALKGDNVVNESPNMTWYPGSFRMPVQWVNRPDLDFRGFSGQIVSGVVRPGDRVRVLPSGRESSVSRIVTMDGDLAEAIAGQSVTITLADEIDISRGDVLAHVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMVR",
    "response#2": "LTCGSVDDGKSTLIGRLLHDSKMIYEDQLEAIKADSVKSGTTGNDFDLALLVDGLQAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRNMATGASNCELAIILIDARYGVQTQTRRHTFIASLLGIKHIIVAVNKMDLMDFSEDVFNTIKQDYIEFTKELNTGDLYFIPMSALNGDNVVNRGQLRFLTCGSVDDGKSTLIGRLLVDSRRVFDDQLAALVRDSRRFGTTGGDVDYALLLDGLEAEREQGITIDVAYRFFATARRSFVVMDAPGHEQYTRNMATAASTAELAVLLVDARKGLLDQTRRHAAICALFRVRHVVLAVNKIDLVDFSQDVFDRIVADFTAFAAPFGFASMTAVPVSARFGDNVVAASPSTPWHAGPTLLGALETADVERDLLRLITCGSVDDGKSTLLGRLLYDCKLLFEDQLASLQSDSARFGSTGTGELDLALLVDGLAAEREQGITIDVAYRFFTTDKRKFIVADTPGHEQYTRNMATGASTAEVAILLVDARKGMLTQTRRHSYIAALLGIRHLVLAVNKMDLVDWQDGIFDTIAADFKSFAANLGEMQVTCIPVSALTGDNIHFEAMVVWMSEVPLDPAKSYHIKHTSNMTSISIDEIRYKIDVNTMHRLNTDSLTLNEIGRVVFSSRKKIFFDPYSKNKAVGSFIIIDKISNNTIAAGMVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGM",
    "response#3": "KSLLRFLTCGSVDDGKSTLIGRLLYDSQLILDDQLASLRKESRNRTTGDEGIDFSLLVDGLTAEREQGITIDVAYRFFSTDKRKFIVADTPGHEQYTRNMATGASNADLGIVLIDARKGVLTQTRRHSFILSLIGVKHVVLAVNKIDLVGYDADVFNAIEAEYRAFAKDLGFETLVAIPLSALKGDNILDPSPQTPWYNGPQLVPYLESIEVATDKSMLRFITCGSVDDGKSTMIGRLLYESKLVFEDQLSALETDSRKSGTQGANLDFALLVDGLAAEREQGITIDVAYRFFTTTKRKFIVADTPGHEQYTRNMVTGASTADLAVLLVDARKGVLQQTRRHSYLVSLLGIRHVVLAVNKLDLVDYSQEVFDAIDRDFRAFAGRIGAPSVVTVPMSALMGDNLTGPSPNTPWYQGPSLIEHLESVEIDDDRQARPFRFPVQYVNRPNLDFRGFAGTVASGSISQGDEIVVAKSGKASRVRRIVAHGGDLRTAVAGQAITLVLEDEVEVSRGNVLHAVADKFEATVVWMSDDPLTPGKEYLIKHASKVAPGRISTLRYRIDVNTLHRADTPTLALNEIGRCHIKLNQPIAFDGYRSNRSTGAFILIDRVTNVTVGAGMIRR"
  },
  {
    "reference": "MAELTSIILAAGQGTRMNSSLPKVLHPLLGRPMLQYIVDASIKVGSSRTIVVVGFEGQQVIDTIDQGHGLGQGQAQAQAHDLQYVWQKQQLGTGHAVMMAQEILSKVDGDLLVLYGDTPLLRAETIQELLKTKRTQQAAAAVLTTQLVEPKGFGRIIRNSAGTIQGIIEEKDASTEERKITEVNTGVYCFEIPLLLKALHEITPRNAQGEYYLTDVIKVMAGKGLRVIGVVTAAAEEVMGPNDRIQLAQTENFLKQAINQRWMSSGVTIQDPSFTYIGPEVEIGRDTTVLPGTFLQGKTIIGEHCIIGPHTKIIDSVIEHHTTVQFSQIVQAHLGPENNVGPYTYVRPGTVSAAKVKIGDFVEIKNCQIGIGTKVPHLTYLGDSELGAGVNIGAGTITCNYDGVSKHRTVIKDGAFIGSNANLVAPVTIGEKATIGAGSTITKEVPDNALAVARSKQEVKTGWRSPREREK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must be able to perform glucosamine-1-phosphate N-acetyltransferase activity under specific conditions. The designed protein sequence is ",
    "response#1": "GVTLKDPARFDVRGEVTTGRDVTIDINVILEGKVVIGDDVTIGPNTIIRDTEIGAGTTIQANCVIEEAVVGANCRIGPFSRLRPGAHLADRVHVGNFVEIKKSQVGEGSKVNHLTYVGDARIGRGVNVGAGTVTCNYDGVNKHPTEIGDAVFIGSGTMLVAPVTIGAGGTIGAGSTITKDTPPDRLTVERSRR",
    "response#2": "AMTSGCTLIDPASVYFSWDTKLGRDIVIEPNVFFGPGVSVADSAIIKAFSHIEGAKISEGAIIGPFARLRPGTKVGAEARVGNFVEVKNSNIAMGAKISHLSXIGDARIGKDTNIGAGTXTCNFDGKNKYRTXIGXEVFVGSNTALXAPLNIGHXVTIGAGSVITQNIKTKSLAXTRVKF",
    "response#3": "GVTLKDPARFDVRGEVTTGRDVTIDINVILEGKVVIGDDVTIGPNTIIRDTEIGAGTTIQANCVIEEAVVGANCRIGPFSRLRPGAHLADRVHVGNFVEIKKSQVGEGSKVNHLTYVGDARIGRGVNVGAGTVTCNYDGVNKHPTEIGDAVFIGSGTMLVAPVTIGAGGTIGAGSTITKDTPPDRLTVERSRG"
  },
  {
    "reference": "MKLGELLKRVRFESYEGSQELDLKSVSYDSRTVQDGALYVAITGLRTDGRRFAKEASASGAAAIASESIEDLPDFKARIKINDARKFLAEASACFTGYPDREMTIIGVTGTNGKTTITYLLESILRAGSRKTGVIGTTGFFNGESWEKLSHTTPESADLWNILKGMKDKGCDTVAMEISSHSLELDRVWGLDVDIAVFTNLTQDHLDFHKDMNSYKAAKFKLFDNIKKEGISIVNADDQTGLELIERLNGKRVVSYSTSGRGADLWVELEYSTIKGSAAVIHYRGLALPCFLGLPGGHNISNLAAASGAALTLGIEPETLKIGVEALKCVPGRFEEVENDKGFFIFVDYAHTPDALERLIKSARELAPRRVITLFGCGGDRDKGKRPMMGRIASGLSDYVFVTSDNPRTENPDEIIREILAGVDGNNNTVIVDRREAIHAAVRFLEKDDVLLVAGKGHEDYQILGTEKIHFDDKEVAKEAVDALP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that can modulate the overall flux of metabolites through the peptidoglycan biosynthesis, Cell wall biogenesis pathway, enhancing or suppressing its activity as needed.2. The designed protein should have a high specificity for ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding, with minimal off-target effects.3. A protein that contributes to cell cycle, cell division, cell wall organization by functioning in cytoplasm with magnesium ion binding, ATP binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, tetrahydrofolylpolyglutamate synthase activity.4. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..5. The presence of Mur ligase C-terminal, Mur ligase N-terminal catalytic, Mur ligase central domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MKLSELLNNIEYNSVEGDMEITHLSIDSRKTKRGSLFFCIKGYQMNGHQFAVEAEAKGAAAIVAEDRIDAGVPVVYVSDTRRALAMIADYYYNNPSKEIKLIGITGTNGKTTTSYLTKSIIEASGKKAGLVGITGTNGKTSSTLLLKQVLETVLGHKAAVIGTIGAIIGNEKLEARNTTPESLDLQRIFADMVDNEVDTVIMEVSSHSL",
    "response#2": "MKEITGITLDSREVMPGFLFGAMPGSNVHGAEFAQKAVENGAAAILTDRAGEALAREAAEGTNTVVVLTEGPRQALSRAAALASAVFGHPSRELVLVGITGTNGKTSSTLLLKQVLETVLGHSTGLIGTIETRIGNEVIPAERTTPESLDLQEMLRVMVDAGVSTVAMEASSHGLAQHRLDGVHFDTAVFTNLTHDHLDYHHTMEEYRAVKARLFQGLEGVAVVNADDPYGRRLVREAKCPVWTYGLGEGADLHATDLTVSVSGTRFTLVTPSGSADIPLRLTGRYNVHNALAAAAAGLVLGVEIPAVCEALAQTPGVP",
    "response#3": "MRLEQLLHGLEYRLLEGDPEEITAICYDSRKAEPGCLFVAVSGFAADGNRFVPMALERGAAAVVTAKKRLEEAPEGVLLVADPRRPLALAAARFYGHPARRLRLAGIGITGTNGKTTTSYLTKSIIEASGLRAGLIGTLGAVIGEERLPAESTTPEPLVLQRLLRRMAAEGVRAVVMEVSSHALALHRVDRCDFEVAVFTNLTQDHLDFHGTMEEYLRAKLRLFTGLGAGGPGRKYAVLNLDDPAGRRLWEGTPAPVLSYGVQGPADLRAERVRLTPGGSRFVLETPVGPLEVETRLVGRFNAYNLLAAFGVGLLLGVPLEEVVRALSRVRVPAGRLQRVGSGPRGGEAYVDYAHTPDGLETVLLALRPHVRGKLIVVFGAGGDRDRGKRPIMGRIASQLADVAIVTDDNPRSEDPAAIRGEVLAGTAGAREIGDRREAIRAAAALLGDGDILVVAGKGHETYQLVGGEKHHFDDREEAAAALEERL"
  },
  {
    "reference": "MVKVGVNGFGRIGRLVTRAAICSGKVEIVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQERDPTNIKWGEAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEDQVVSCDFNSNSHSSTFDAGAGIALNDNFVKLISWYDNEYGYSNRVVDLMAYMASKE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should include a [IL]-x-C-x-x-[DE] motif motif to ensure proper function.2. The protein design should take into account the physiological conditions of the Carbohydrate degradation, pyruvate from D- glyceraldehyde 3-phosphate: step 1/5, glycolysis pathway.3. The ideal protein would be one that plays a critical role in microtubule cytoskeleton organization, response to ammonium ion, canonical glycolysis, cAMP-mediated signaling, killing by host of symbiont cells through disordered domain specific binding, identical protein binding, enzyme binding, NADP binding, microtubule binding within ribonucleoprotein complex, postsynaptic density, intracellular component, glutamatergic synapse, cytosol, GAIT complex. The designed protein sequence is ",
    "response#1": "IGKGCSAAVYEATMPVLPQNQQVAGSIGLLPGRGPEIIPRGEEEQAPLAPAFPLAIKMMWNISAGSSSEAIFSTMSQELVPASRVALAGEYGAVAHRRSKGGPKQLAAHPNVIRVFRAFTSSVPLLPGALLDYPDVLPPRLHPEGLGHGRTLFLVMKNYSCTLRQYLRGNSPSPRLATVMTLQLLEGVAHLVQQGVAHRDLKSDNVLVEPDADGCPWLVITDFGCCLADERVGLQLPFTSWYVDRGGNGCLMAPEVSTACPGPRAVIDYSKADAWAVGALAYEIFGLPNPFYGQGSRAHLESRSYQEAQLPALPESVPPEARQLVRSLLQRDTSKRPSARVAANLLHLSLF",
    "response#2": "AAHPNVIRVFRAFTSSVPLLPGALLDYPDVLPPRLHPEGLGHGRTLFLVMKNYSCTLRQYLRGNSPSPRLATVMTLQLLEGVAHLVQQGVAHRDLKSDNVLVEPDADGCPWLVITDFGCCLADERVGLQLPFTSWYVDRGGNGCLMAPEVSTACPGPRAVIDYSKADAWAVGALAYEIFGLPNPFYGQGSRAHLESRSYQEAQLPALPESVPPEARQLVRSLLQRDTSKRPSARVAAHPNVIRVFRAFTSSVPLLPGALLDYPDVLPPRLHPEGLGHGRTLFLVMKNYSCTLRQYLRGNSPSPRLATVMTLQLLEGVAHLVQQGVAHRDLKSDNVLVEPDADGCPWLVITDFGCCLADERVGLQLPFTSWYVDRGGNGCLMAPEVSTACPGPRAVIDYSKADAWAVGALAYEIFGLPNPFYGQGSRAHLESRSYQEAQLPALPESVPPEARQLVRSLLQRDTSKRPSARVCH",
    "response#3": "MVLRFANRIFGPIWNRDNIACVILTFKEPFGTEGRGGYFDEFGIIRDVMQNHLLQMLCLVAMEKPASTDSDDVRDEKVKVLKCISEVETDNVVLGQYVGNPSGEGEATNGYLDDPTVPHGSTTATFAAAVLYVENERWDGVPFILRCGKALNERKAEVRLQFRDVAGDIFHQQCKRNELVIRVQPNEAVYTKMMTKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHKIDREKPQPIPYVYGSRGPTEADELMKRVGFQAAHPNVIRVFRAFTSSVPLLPGALLDYPDVLPPRLHPEGLGHGRTLFLVMKNYSCTLRQYLRGNSPSPRLATVMTLQLLEGVAHLVQQGVAHRDLKSDNVLVEPDADGCPWLVITDFGCCLADERVGLQLPFTSWYVDRGGNGCLMAPEVSTACPGPRAVIDYSKADAWAVGALAYEIFGLPNPFYGQGSRAHLESRSYQEAQLPALPESVPPEARQLVRSLLQRDTSKRPSARVQKQDIVRVTQTLLDAISCKDFDTYTRLCDTSMTCFEPEALGNLIEGIEFHRFYFDGNRKNQVHTTMLNPNVHIIGEDAACVAYVKLTQFLDRNGEAHTRQSQESRVWSKKQGRWLCVHVHRSTQS"
  },
  {
    "reference": "MSGTGGLGVIILAAGAGTRMNSERPKVLHELAAAPLLHHAMRSAAALAPEQMVVVTGVGAAEVEKAARAFDPDVSTVHQAERLGTAHAVLQARDLLGQHDGDVIVLYGDTPFVTPETLERMMEARSRADIVVLGFETADSDARYGRLVTHGDVLERIVEWKDADAATRAIRLCNSGVVCAEGTFLFDLLREVGNDNAAGEYYLPDVVAIAAGRGMTTTVVRCEEPETLGINSRAELMAAERAFQARIRAQALEDGVSMQAPETVIFAYDTFVGRDTIIEPNVVFGPGVTVESGARIRAFSHLEGCHVSRGATVGPFARLRPGAELAEDVHVGNFVEIKAAQIGEGAKVNHLSYIGDADVGERTNIGAGTITCNYDGVFKHRTIIGRDAFIGSDTMLVAPVRIGDGAMTASGSVITKDVAPGSLGLARARQVEKPAFATKLFEKLRALKAARSGA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein have enhanced acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.2. The protein requires Mg(2+) to activate a critical catalytic site.3. A protein with magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity is needed to enhance UDP-N-acetylglucosamine biosynthetic process, lipid A biosynthetic process in a specific context.4. The UDP-N-acetyl-alpha-D-glucosamine binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MAAGLGKRMRSETPKHLHPLLGRRLVDWVIETALALGAKPLVVVTSPQTEDTIAHVGVTVAVQPEARGTGDAIAAARTALAGFDGDVLVLSGDSPLLTPDLLQRLVDAHRESEAAATVLTFEPVRTLPYGRVVRDGDGNVRAIVEDGDANEQERSIRELNSSTYVFVARELWGALDELTPEDANDREIKLCNAGMMAVRGADLFRLLDAVGNDNAKGEYYLTDAVEIARGDGRKAAAVVAPADEVLGCNDRAQLAAAEAAFQARKRAAMQHEQIVVGPRAAIDPGAELTGPCRILGATTIGQGATIGAFCQITNCVIDAGATVREFCHLEDAHVCAGAVAGPYSRLRPGADVGENARVGNFVEMKKASLGKGAKASHLTYLGDTAVGAGANIGAGTITCNYDGVHKHQTNIGAGAFIGSNTALVAPVQVGENALVAAGSVITKTVPDGALAVARARQEVKPEWREHFRARMKAAKGKKG",
    "response#2": "MAAGLGKRMRSETPKHLHPLLGRRLVDWVIETALALGAKPLVVVTSPQTEDTIAHVGVTVAVQPEARGTGDAIAAARTALAGFDGDVLVLSGDSPLLTPDLLQRLVDAHRESEAAATVLTFEPVRTLPYGRVVRDGDGNVRAIVEDGDANEQERSIRELNSSTYVFVARELWGALDELTPSMGEFNINDLARAEALFQDVRREDFMKAGVTLRDPKTVWFSWDTEIGKDVTIFPNVVFGPGVSIADKVEIRAFCDIEDAVIGEGASIGPFARIRAGAELGRDVHLGNFVEVKKSRIGDGTKAGHLSYLGDAEIGTKTNIGAGTITCNYDGVNKDKTIIGNNVFIGSNASLVAPVSIGDGAYTASGSVITEDVPADALALGRARQVNKKEWLRKRHLARAEALFQDVRREDFMKAGVTLRDPKTVWFSWDTEIGKDVTIFPNVVFGPGVSIADKVEIRAFCDIEDAVIGEGASIGPFARIRAGAELGRDVHLGNFVEVKKSRIGDGTKAGHLSYLGDAEIGTKTNIGAGTITCNYDGVNKDKTIIGNNVFIGSNASLVAPVSIGDGAYTASGSVITEDVPADALALGRARQVNKKGWLRKQ",
    "response#3": "MAAGLGKRMRSETPKHLHPLLGRRLVDWVIETALALGAKPLVVVTSPQTEDTIAHVGVTVAVQPEARGTGDAIAAARTALAGFDGDVLVLSGDSPLLTPDLLQRLVDAHRESEAAATVLTFEPVRTLPYGRVVRDGDGNVRAIVEDGDANEQERSIRELNSSTYVFVARELWGALDELTPYDVTEIIPLARAEALFQDVRREDFMKAGVTLRDPKTVWFSWDTEIGKDVTIFPNVVFGPGVSIADKVEIRAFCDIEDAVIGEGASIGPFARIRAGAELGRDVHLGNFVEVKKSRIGDGTKAGHLSYLGDAEIGTKTNIGAGTITCNYDGVNKDKTIIGNNVFIGSNASLVAPVSIGDGAYTASGSVITEDVPADALALGRARQVNKPGWADRRREHQDG"
  },
  {
    "reference": "MQIILLEKVVNLGNLGDVVRVKDGYARNFLIPNKQARRATASAIQEFEARRAELEKLAAERLAAAQAEGEKLNGLTLQLSQKAGVDGRLFGSVTNHDIAAALTAQGFKVEKAQVRMPNGPLKTVGDHPVAVSLHTDVSVDVTVSVLGETV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have structural constituent of ribosome within ribonucleoprotein complex, ribosome to regulate translation.2. For general function, the protein need meet that Binds to the 23S rRNA. The designed protein sequence is ",
    "response#1": "MPIGVPKVPFR",
    "response#2": "MAAAQAVADKMNGTVVTVARKAGMDGRLFGSVGNADIADALKAAGYVVDKSAVRMPEGPLKAIGEFPLDVALHTDVLANITVAIVL",
    "response#3": "MEPGNLGDIVKVKDGYARNFLIPQRMAKRAD"
  },
  {
    "reference": "MKRKLVFATNNLHKLEEVSAILGNQIELLSLNDINCHIDIPETADTLEGNALLKSRFIYENYGMNCFADDTGLEVEALEGAPGVYSARYAGDGHNSEANMIKLLDNLKEKKNRKAQFRTAISLIIDEKEYLFEGIIKGEIGTIKKGDSGFGYDPIFVPEGYNETFAELGSDIKNKISHRALAINKLCEFLHSL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must bind to Mg(2+) in order to perform its enzymatic function.2. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.3. The protein design must be able to perform XTP diphosphatase activity in order to contribute to purine nucleoside triphosphate catabolic process. The designed protein sequence is ",
    "response#1": "MPLLDSQGRVINTWADIINRANLGMEVMHERNAHNFPL",
    "response#2": "ME",
    "response#3": "MPTV"
  },
  {
    "reference": "MISKNATKYIFVTGGVLSSLGKGITAASIGTLLKHTGQRVAVLKLDPYINVDPGTMSPLEHGEVFVTKDGAETDLDLGHYERFLDTSLTQNNNFTTGLVYKTVIENERKGKYLGKTIQVVPHIVNEIKERVYKAGEGRDILIVELGGTTGDIEGLPFLETIRQIKHELPRNRVMNIHVTLLPYIKAAGELKTKPTQHSIQELRRIGISPHMLVLRAEVPVSTEIKHKIAYSCDVDNESVIVAEDAATIYKVPLNFLAQDVLTPICKQLDLENCTPKMDEWSHLVSRIIMPENEIKIAFVGKYLDLKESYKSLTEALIHSGAHLDTKVNIKWVDSEKVDDDGAEKYLNDCDGILVAGGFGSRGVEGKIKAIEYARVQKIPFLGICLGMQLSMIEFARNVLGIEDANSVEFDENTANPIVYLIDEFIDTAGAKQVRTTTSAMGGTLRLGEYECITKEGSKLREAYDSAMIYERHRHRYEANPKYREALESNGMRVTGESHGLIDAMEVTDHPWFLGVQFHPEFTSRLQNPNPAIVAFVNASLSHRKTEV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein with metal ion binding, ATP binding functions is required to enable glutamine metabolic process, 'de novo' CTP biosynthetic process.2. The Nucleophile, active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The protein should have a high affinity for L-glutamine, Mg(2+). The designed protein sequence is ",
    "response#1": "LPDAYLSVVEALYHGGLAHGIGVDIRWVAAGELENRRAEDILDGANGILVPGGFGDRGVEGKISAIHYARENRIPFMGICLGMQLAAVEYARNVCKLADANSTEFDPHTAHPVVDLLPEQKEIEAMGGTMRLGVYPCRIERDSLAHKVYEQDLIYERHRHRYEFNKYYRNILESHGMTFSGFSPDNRLMEVVELRDHPWFLGCMFHPGYKSRPTRPHPLFKGFVE",
    "response#2": "VRIALVGKYTELADAYLSHLEAFHHAGAELKTKVDIRFFDSELVEKNGISKELACCDGILIPGGFGSRGTEGKIATALFAREKGIPFLGVCLGFQIATIEIARNVLGLPDAHSTEFKPECETPVIDILPEQKGVLTMGGTMRLGSQKVFVKEGSAAHQLYGRTEIAERHRHRYEVNPDYIDRLEEAGWKFTGRSDDGVKMEIGELEGHPFYMAAQFHPEFKSRPRKPSPLHLGLVTIGMVGKYIELTESYKSLNEALTHAGIHTTTKVNIRYLDSEKIETEGPECLQGLDGILVPGGFGVRGVEGKIMAARYAREHTIPYLGICLGMQVAVIEYARHVAGLEGAHSTEFNLDTPHPVIALITQWQGHDGRLEQRDASSDLGGTMRLGGQECRLRPQTLAQQTYAKELIMERHRHRYEFNNDYLAHLKEAGLVIAGTSRDGHLVEMVELHDHPWFVGCQFHPEFTSTPRDGHPLFAGFVHRYNA",
    "response#3": "VTIAIVGKYTSLKDSYKSLSEALAHGGIANRIKVNLKFLDSELFEHEDETTYLHHLEDVGGILVPGGFGLRGAEGKIEAIRIARERNIPYFGICFGMQMAVLETARALGGMDKASSTEFGPCDDALVGLMTEWTAGNKVQTRADGDDLGGTMRLGAYDCXLEEGSLVRSIYDAEHIQERHRHRYEVNVAYRDKLXNAGMVMSGVSPDGALPEIIERPDHPWFVGVQFHPELKSKPFEPHPLFTGFR"
  },
  {
    "reference": "MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGGIMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVLPAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAIVKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFARLLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALINGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVAYLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGLRALAAATLSNLMSATIAGFFIGLA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design must be able to perform symporter activity in order to contribute to nucleoside transmembrane transport.2. The Cytoplasmic, Periplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells. The designed protein sequence is ",
    "response#1": "PDAYKSLIEALNHGAISNKVKIKINWVNSREENEKPINKNLIGEKLQDSHAILVPGGFGDNGTEGKMLAVSYARTHNIPFFGICLGMQLAVIEFARNVIKLEDVHSEEFCTCKHPIVKLAGDQSVNLGGTMRLGAYKCNISPNSKMADIYSSVTILERHRHRFIINLDYKDDLEKNGLICSSMSEDGTCIEAVELENHPWFIGVQFHPEFQSKPFSPHPLFVSFIY",
    "response#2": "PDAYKSLIEALNHGAISNKVKIKINWVNSREENEKPINKNLIGEKLQDSHAILVPGGFGDNGTEGKMLAVSYARTHNIPFFGICLGMQLAVIEFARNVIKLEDVHSEEFCTCKHPIVKLAGDQSVNLGGTMRLGAYKCNISPNSKMADIYSSVTILERHRHRFIINLDYKDDLEKNGLICSSMSEDGTCIEAVELENHPWFIGVQFHPEFQSKPFSPHPLFVSFIY",
    "response#3": "PDAYKSLIEALNHGAISNKVKIKINWVNSREENEKPINKNLIGEKLQDSHAILVPGGFGDNGTEGKMLAVSYARTHNIPFFGICLGMQLAVIEFARNVIKLEDVHSEEFCTCKHPIVKLAGDQSVNLGGTMRLGAYKCNISPNSKMADIYSSVTILERHRHRFIINLDYKDDLEKNGLICSSMSEDGTCIEAVELENHPWFIGVQFHPEFQSKPFSPHPLFVSFIY"
  },
  {
    "reference": "MDYLPIFADLKHRPVLVVGGGEIAARKIGLLLSAGAEVFVVAKSLGNEVSQWHLTQRIHWLAQSFEVSQLDDIFLVVAATNDSTLNARVFQAANQRRRLINIVDDPARCSFIFPSIIDRSPLVVAVSSGGKAPVLARLLREKLEALLPTSLGKVAQIAGNWRGQVKQHLKTLGERRGFWERVFTGRFAALVAANRIDDAEKELEQQLYHADERTGDVTLVGAGPGDAGLLTLRGLQAIQQADVVLHDALVTEEILELVRRDAQLISVGKRAGAHSVAQEETNRLLVELAQQGKKVVRLKGGDPFIFGRGGEELEVLADAGIPFQVVPGITAAAGVTAYAGIPLTHRDRAQSVTFITGHCKPGTDGLNWSQLAQGHQTLAIYMGTVKASEIQQQLIRHGRAASTPIAVIGRGTRADQQVLSGTLQQLDELAKQAPTPALLVIGEVAALHHKLKWFGESVSQTPQDNLTLWPSVVNLA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should be able to enhance the efficiency of cobalamin biosynthetic process. The designed protein sequence is ",
    "response#1": "VGAGPGDPDLLTFRALRLMQKADVVVYDRLVSPAIMELVRRDAEVIYVGKERAKHTMKQENINQLLVRLAKQGKRVLRLKGGDPFIFGRGGEEIETLAEEQVPFQIIPGITAAIGCSSYAGIPLTHRDYAQSCVFVTGHLKDGSVDLNWKALAHPDQTVVFYMGLHGAPTLCKELVAHGLPETTPVALVEQGTTPQQRVFTATLATLLDVIANEDIQPPTLIIVGEVAE",
    "response#2": "VGAGPGDPDLLTFRALRLMQKADVVVYDRLVSPAIMELVRRDAEVIYVGKERAKHTMKQENINQLLVRLAKQGKRVLRLKGGDPFIFGRGGEEIETLAEEQVPFQIIPGITAAIGCSSYAGIPLTHRDYAQSCVFVTGHLKDGSVDLNWKALAHPDQTVVFYMGLHGAPTLCKELVAHGLPETTPVALVEQGTTPQQRVFTATLATLLDVIANEDIQPPTLIIVGEVTQ",
    "response#3": "VGGGPGDPDLLTFKALRLMQQADIVLYDRLVSPQVLDKVRRDAELVYVGKRAGNHAVSQDNINLKLVEYAQQGLRVLRLKGGDPFIFGRGGEEIATLAEYNIPFQVVPGITAASGCASYAGIPLTHRDHAQSVRFIAGHLMSGELNLDWAELAKPAQTLVFYMGLSGMETICSQLMAHGAPGGRAAAIIEKGTQPDQRNFVADLATLPALVRAAQAGAPTLLIVGEVI"
  },
  {
    "reference": "MAKVLCVLYDDPVDGYPKTYARDDLPKIDHYPGGQTLPTPKAIDFTPGQLLGSVSGELGLRKYLESNGHTLVVTSDKDGPDSVFERELVDADVVISQPFWPAYLTPERIAKAKNLKLALTAGIGSDHVDLQSAIDRNVTVAEVTYCNSISVAEHVVMMILSLVRNYLPSHEWARKGGWNIADCVSHAYDLEAMHVGTVAAGRIGLAVLRRLAPFDVHLHYTDRHRLPESVEKELNLTWHATREDMYPVCDVVTLNCPLHPETEHMINDETLKLFKRGAYIVNTARGKLCDRDAVARALESGRLAGYAGDVWFPQPAPKDHPWRTMPYNGMTPHISGTTLTAQARYAAGTREILECFFEGRPIRDEYLIVQGGALAGTGAHSYSKGNATGGSEEAAKFKKAV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be able to perform oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor under specific conditions.2. The designed protein should have a high specificity for NAD(+) binding, with minimal off-target effects.3. For general function, the protein need meet that Catalyzes the NAD(+)-dependent oxidation of formate to carbon dioxide; Formate oxidation is the final step in the methanol oxidation pathway in methylotrophic microorganisms; Has a role in the detoxification of exogenous formate in non-methylotrophic organisms. The designed protein sequence is ",
    "response#1": "TLQLVRNIIPPLSSTKHKGQDGRIGVVGGCQEYTGAPYFAAISALKVGADLSHVFCASAAAPVIKAYSPELIVHPVLDSPNAVHEVEKWLPRLHALVVGPGLGRDDALLRNVQGILEVSKARDIPVVIDADGLWLVAQQPALIHGYRKAVLTPNHVEFSRLYDAVLRGPMDSDDSHGSVLRLSQALGNVTVVQKGERDILSNGQQVLVCSQEGSSRRCGGQGDLLSGSLGVLVHWALLAGPQKTNGSSPLLVAAFGACSLTRQCNHQAFQKHGRSTTTSDMIAEVGAAFSKLFAD",
    "response#2": "GVVGGCQEYTGAPYFAAISALKVGADLSHVFCASAAAPVIKAYSPELIVHPVLDSPNAVHEVEKWLPRLHALVVGPGLGRDDALLRNVQGILEVSKARDIPVVIDADGLWLVAQQPALIHGYRKAVLTPNHVEFSRLYDAVLRGPMDSDDSHGSVLRLSQALGNVTVVQKGERDILSNGQQVLVCSQEGSSRRCGGQGDLLSGSLGVLVHWALLAGPQKTNGSSPLLVAAFGACSLTRQCNHQAFQKHGRSTTTSDMIL",
    "response#3": "GVVGGCQEYTGAPYFAAISALKVGADLSHVFCASAAAPVIKAYSPELIVHPVLDSPNAVHEVEKWLPRLHALVVGPGLGRDDALLRNVQGILEVSKARDIPVVIDADGLWLVAQQPALIHGYRKAVLTPNHVEFSRLYDAVLRGPMDSDDSHGSVLRLSQALGNVTVVQKGERDILSNGQQVLVCSQEGSSRRCGGQGDLLSGSLGVLVHWALLAGPQKTNGSSPLLVAAFGACSLTRQCNHQAFQKHGRSTTTSDML"
  },
  {
    "reference": "MRVLKFGGTSVANAERFLRVADILESNAQQGQVATVLSAPAKITNHLVAMIEKTISGQDALPNITDAERIFDELLQGLAAAQPGFDYDGQKTRVDLEFAQLKQVLHGISLLGQCPDSVNAAIICRGEKLSIAIMEALLLARGHGVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASQIPPQNMILMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTIAPIAQFQIPCLIKNTANPQAPGTLIGGEGDQDENPVKGITNLNNMAMFNVSGPGMKGMVGMAARVFAAMSRTGISVVLITQSSSEYSISFCVPQNEQARARRVLEEEFYLELKDGLLDPLGVMEQLAVISVVGDGMRTLRGISAKFFSALARANINIVAIAQGSSERSISVVVSNDDATTGVRVVHQMLFATDQVIEVFVVGVGGVGGALLDQLYRQQAWLKQKHIDLRVCGIANSRALLTNVHGIDLSDWKGALSEAKEPFNLDRLNRLVKEYHLLNPVIVDCTSSQAVADQYADFLADGFHVVTPNKKANTSSWNYYQQMRAAAAKSRRKFLYDTNVGAGLPVIENLQNLLNAGDELIKFTGILSGSLSFIFGKLDEGVSLSEATKMAREMGFTEPDPRDDLSGMDVARKLLILAREAGHKLELSDIEIEPLLPASLNEIADVEEFMQRLPELDNAFAARVVKARDEGKVLRFVGAIEEGGVCKVKIDAVDSNDPL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should take into account the physiological conditions of the L-threonine from L-aspartate: step 3/5, Amino-acid biosynthesis, L-threonine biosynthesis pathway.2. A protein with aspartate kinase activity, ATP binding, homoserine dehydrogenase activity, NADP binding functions is required to enable lysine biosynthetic process via diaminopimelate, threonine biosynthetic process, phosphorylation.3. The designed protein have ACT domains with specific sequence motifs that are crucial for its activity.4. The protein have enhanced L-homoserine + NADP(+) = H(+) + L-aspartate 4-semialdehyde +   NADPH. The designed protein sequence is ",
    "response#1": "MRVLKFGGTSVANAQRFLCVAEILESKAQQEQVATVLSAPAKITNHLVAMIEKTISGQDAMPNISDAGQIFADLLNGLAQLQPGFPLAEMKSVVEHEFAQIKHVLHGISLLGQCPDSINAAFISRGEKLSIAIMAALLTARGHQVSVIDPVEKLLANGHYLESTVDIPESTRRIAASRIPAENMILMAGFTAGNEKNELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPDAPGTLIGATKDDDNLPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRNGISVVLITQSSSEYSISFCVPQSECVRAAKALEDEFYLELKEGLLEPFMITERLAIISAVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVSNDDAMTGVRVTHQMLFNTDQVVEVFVIGVGGVGSALIEQIKRQQPWLKRKHIDLRVCGVANSRALLTSVHGLDLENWQSMLGEAKEPFNLGRLIRLVKEYHLLNPVIVDCTSNQSVADQYVDFLREGFHVVTPNKKANTSSMAYYHELRQAAEKSRRKFLYDTNVGAGLPVIENLQNLLDAGDELQHFSGILSGSLSFIFGKLDEGVSLSEATRVARELGYTEPDPRDDLSGMDVARKLLILARENGHELELDDIIVEPVLPPEFDSSGDVTQFMARLPELDDAFSARVAKARDEGKVLRYVGQIQEDGRCVVKIDAVDGNDPFS",
    "response#2": "MRVLKFGGTSVANAQRFLCVAEILESKAQQEQVATVLSAPAKITNHLVAMIEKTISGQDAMPNISDAGQIFADLLNGLAQLQPGFPLAEMKSVVEHEFAQIKHVLHGISLLGQCPDSINAAFISRGEKLSIAIMAALLTARGHQVSVIDPVEKLLANGHYLESTVDIPESTRRIAASRIPAENMILMAGFTAGNEKNELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPDAPGTLIGATKDDDNLPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRNGISVVLITQSSSEYSISFCVPQSECVRAAKALEDEFYLELKEGLLEPFMITERLAIISAVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVSNDDAMTGVRVTHQMLFNTDQVVEVFVIGVGGVGSALIEQIKRQQPWLKRKHIDLRVCGVANSRALLTSVHGLDLENWQSMLGEAKEPFNLGRLIRLVKEYHLLNPVIVDCTSNQSVADQYVDFLREGFHVVTPNKKANTSSMAYYHELRQAAEKSRRKFLYDTNVGAGLPVIENLQNLLDAGDELQHFSGILSGSLSFIFGKLDEGVSLSEATRVARELGYTEPDPRDDLSGMDVARKLLILARENGHELELDDIIVEPVLPPEFDSSGDVTQFMARLPELDDAFSARVAKARDEGKVLRYVGQIQEDGRCVVKIDAVDGNDPFV",
    "response#3": "MRVLKFGGTSVANAQRFLCVAEILESKAQQEQVATVLSAPAKITNHLVAMIEKTISGQDAMPNISDAGQIFADLLNGLAQLQPGFPLAEMKSVVEHEFAQIKHVLHGISLLGQCPDSINAAFISRGEKLSIAIMAALLTARGHQVSVIDPVEKLLANGHYLESTVDIPESTRRIAASRIPAENMILMAGFTAGNEKNELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPDAPGTLIVSGPGMKGMVGMAARVFAAMSRNGISVVLITQSSSEYSISFCVPQSECVRAAKALEDEFYLELKEGLLEPFMITVSGPGMNSMVAVASRVLSSMSLKKIRITLITQSSSEFNISFCTSQEYVKKIKNTLENENLHKFGGSSLADPACYRRVAQILKEYSQSHDLVVVSAAGKTTNQLIAFIENLTVEPQQAQEILGQIQHFQHQLIADLLEGEAAQALTNRLTDECMTLAELRAPLDDAQQAFVLAHGEIWSSRLMAALLNQQGLMAIAQDARAFLRAHRETQPEIDRAQSYPLLKEILAQHGHQRIVMTGFIAQDEAGETVLLGRNGSDYSATIIGALAEVERVTIWSDVAGVYSADPRLVTDACLLPLLRLDEASELARLAAPVLHSRTLQPVAQSAIDLHLRCSYDPEAGSTHIERVLASGRGAKIITSLDEVLLIELSFSRGHDFQRQYKQVIEQLKRAQLTPLAYDKQEDQYRIQLAYTAEIAAGALSYLQDAAIEAEIRLKEGYSLVAAVGAGVTKNANHCFGFYQQLKQAPIEFLCESELSLVAILRHTSIDTLVNQLHNHLFQAQKRVALALCGKGNIGSSWLKLFAEQKAELEKRRGMNFELVAIIDSQTYWFDERGIDPTQVEQHFLEESTPNDGQGWLLKLGQIRGYDDVIVLDVTASAELAGQYIQIAEQGMHLISANKVAGSSSSEYYYQVKDSFAKISRHWFYNATVGAGLPINHTVRDLRESGDEIVALSGIFSGTLSWLFQQYDGTVPFSELVDLAWQQGLTEPDPRNDLDGSDVMRKLVILARESGLDIEPNQVTVESLVPEELQCLSLDDFLDQSAQLDIQLAERLAKAQREDKVLRYVARLEKSGKASVGVEALHREHPLANLLPCDNIFAIES"
  },
  {
    "reference": "MRKLILATFLLLAPTALLASGGGEHLESANIDLRDQASLQRGAKYFMNYCTGCHSLQYMRYNRLAKDLGIDEIALRQNLLFGDAKPGDLITKAMTDDDALKWFGVVPPDLTLVTRWRSPDWVYTYLKSFYLDDTRPYGVNNVLFPLVGMPHVLGDLQGRQEAVMEPSHEPGGEPTIKGVKLVEEGGLSPQEYDTMVRDITNFLTYAGEPFQLERERIGRYVLLFLGFLFILAYLLKKEYWKDVH",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a high affinity for heme c.2. The protein should contain a Helical membrane-spanning region.3. For general function, the protein need meet that Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis; c1 functions as an electron donor to cytochrome c.4. Ensure the presence of a signal peptide within the protein.5. The protein should have metal ion binding and be active in respirasome. The designed protein sequence is ",
    "response#1": "NPLNTPPHIKPEWYFLFAXAILRSIPNKLGGVLALVLSILILMIIPLLHTAKQRSMMFRPMSQCMFWILVADLLTLTWIGGQPVEYPFVVIGXLASVIYS",
    "response#2": "NPSGLNSNADKIPFHPYYTIKDLLGIFMLLLALMTLVLFFPDILGDPDNYIPANPLNTPAHIKPEWYFLFAYAILRSIPNKLGGVLALLLSILILAAFPLLNTSKQHGLMYRPITQTLYWIFIANLFILTWIGGQPVEHPFIMIGQI",
    "response#3": "GLNSDSDKIPFHPYYTIKDLLGVFILLMVLMFLVLFFPDILGDPDNYTPANPLNTPAHIKPEWYFLFAYAILRSIPNKLGGVLALILSILILAAFPLLNSSKQQGLVFRPITQILYWFLVANLVILTWIGGQPVEYPFTIIGQIASISYFSIIVILXPMASTIENNIMKID"
  },
  {
    "reference": "MKFSTQKPKKTLHMVSLGCTKNLVDSEVMLGRLSDYQLTDDAQNADVIIVNTCGFIDSAKQESINTILSLHEDRKNESVLVMAGCLSERYKEELQKELPEIDVFTGVGDYDRIDELVNEKRSNFTSEVFLASETNERVITGSSYHAYVKLSEGCNQACSFCAIPSFKGKLHSRTLQSLVKEVKALVAKGYVDFSFVSQDSSSFLRDLDIKNGLELLVEEVEKIEGIKTARILYLYPSTTTLSLIDKIADSKVFVNYFDMPLQHITPSMLKIMKRGKGVEQLNELMNHMKSKPNSFVRTTFIAGHPGETEDDFEALCNYVENFKFDRANVFSYSDEEGTTAETRTDKVEQELIDERAEVLGEIISQTTQESLESEVGKTFEVYIDGESEEHEYLLSARKTIWAPSIDGEIYINDNELSEGEQIKFGQIYTVKITELVGDKLLATVIK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must have at least one TRAM, MTTase N-terminal, Radical SAM core domain.2. The ideal protein would be one that plays a critical role in peptidyl-L-beta-methylthioaspartic acid biosynthetic process from peptidyl-aspartic acid, tRNA modification through metal ion binding, 4 iron, 4 sulfur cluster binding, protein methylthiotransferase activity within cytoplasm.3. The presence of [4Fe-4S] cluster is crucial for the protein to function optimally under specific conditions.4. The [4Fe-4S] cluster binding site should be located in a region of the protein that is accessible to the ligand.5. We are looking for a protein with broad substrate specificity for [sulfur carrier]-SH + AH2 + L-aspartate(89)-[ribosomal protein   uS12]-hydrogen + 2 S-adenosyl-L-methionine = 3-methylsulfanyl-L-   aspartate(89)-[ribosomal protein uS12]-hydrogen + 5'-deoxyadenosine +   [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-   homocysteine. The designed protein sequence is ",
    "response#1": "MKVGIISLGCAKNQVDTEVMQGILENSNYKMTDDYYDADIIIVNTCGFIDDAKEESVDHILEVAQLKETGKLKVLIVAGCLSQRYQESLKEEIPEIDAMIGTDTQDKITEVISSALKGNYISFYDRLNKIDEQLFLRQPYQPGPSAYIKIAEGCHNYCSYCAIPLIRGGYRSRTIEDIKIEANHFIEKGSKELTLIAQDTTNYGSDIYGKFSLDTLLDELATIPGDFWIRVLYAYPTRITDSLIEVINRHEKICSYLDIPLQHIDDDILTSMNRGGNKEQILNLIHNLRKNIPDITLRTSLIVGFPGETDEKYQNLISFMQEIEFDHAGIFKYSDEEDTQAYNFKDKVSEDVKEQRYQEAWEVQKEITRKKNEGLVGTEMRVLIEEALEDEPTTKVGRTEGHAPEVDGAVIIPDCEASSGDFINVEIVQALDYDLIGEMTQRKVGKSLRVLVDEVNQDGGIGRSSADAPEIDGLVYIAPPSKPYKRYKAGDFVSVKITGADGHDLWGEA",
    "response#2": "MKVGIISLGCAKNQVDTEVMQGILENSNYKMTDDYYDADIIIVNTCGFIDDAKEESVDHILEVAQLKETGKLKVLIVAGCLSQRYQESLKEEIPEIDAMIGTDTQDKITEVISSALKGNYISFYDRLNKIDEQLFLRQPYQPGPSAYIKIAEGCHNYCSYCAIPLIRGGYRSRTIEDIKIEANHFIEKGSKELTLIAQDTTNYGSDIYGKFSLDTLLDELATIPGDFWIRVLYAYPTRITDSLIEVINRHEKICSYLDIPLQHIDDDILTSMNRGGNKEQILNLIHNLRKNIPDITLRTSLIVGFPGETDEKYQNLISFMQEIEFDHAGIFKYSDEEDTQAYNFKDKVSEDVKEQRYQEAWEVQKEITRKKNEGLVGTEMRVLIEEALEDEPTTKVGRTEGHAPEVDGAVIIPDCEASSGDFINVEIVQALDYDLIGEMTEVGETLQVLVESVESEEDGEVAIGRAAHQAPETDGQVVFTTREGLVPGLMVEAKAVGTEGVDLVAGEGLVGTEMRVLIEEALEDEPTTKVGRTEGHAPEVDGAVIIPDCEASSGDFINVEIVQALDYDLIGEMTEAELAEAEVGQEIPVILEGGSSEHEFFYSARDARWAPEIDGEILINETLLPQPAPGHYWAKITQVAGKQLLAERTRIGRTETVLVDEVVEEGAVARRRADAPEIDGQVFIDGATHLEVGEFVEATFEDADAHDLWARLAE",
    "response#3": "MKVGIISLGCAKNQVDTEVMQGILENSNYKMTDDYYDADIIIVNTCGFIDDAKEESVDHILEVAQLKETGKLKVLIVAGCLSQRYQESLKEEIPEIDAMIGTDTQDKITEVISSALKGNYISFYDRLNKIDEQLFLRQPYQPGPSAYIKIAEGCHNYCSYCAIPLIRGGYRSRTIEDIKIEANHFIEKGSKELTLIAQDTTNYGSDIYGKFSLDTLLDELATIPGDFWIRVLYAYPTRITDSLIEVINRHEKICSYLDIPLQHIDDDILTSMNRGGNKEQILNLIHNLRKNIPDITLRTSLIVGFPGETDEKYQNLISFMQEIEFDHAGIFKYSDEEDTQAYNFKDKVSEDVKEQRYQEAWEVQKEITRKKNEGLVGTEMRVLIEEALEDEPTTKVGRTEGHAPEVDGAVIIPDCEASSGDFINVEIVQALDYDLIGEMTQRKVGKSLRVLVDEVNQDGGIGRSSADAPEIDGLVYIAPPSKPYKRYKAGDFVSVKITGADGHDLWGEE"
  },
  {
    "reference": "MRMPAVTIREMRDAENRAMASGMTEEKILNHAGTRLGHAIAKFFPKQGHAIAYLGKGHNAGDTLVALRILRDFYGWKISSRLSHPIDACAPLTRKKWNELGNAENLSAMPDWQSCDRPLILIDALLGIGAQGPMREPLLAMANEMRWLRENAGARIVAVDLPSGLDADSGTATPGTVTADITLMIGLAKHGLLMSHAATYTGALSLVSVDCLKHSHPTDIELICPQTLHFGKSPRPFDFHKGMAGRVAILAGSTNYPGAAVLAAIGALRGGAGLITLHTPKDAAPLIAPRCPPEIIIRPYSSPLEILETRFDSLVIGCGIGQPDPQTQVDIMRLIGETHTPTVIDADALNLIAANSQTHILDERHLITPHPLEFARLFSELAKISREAAVSSFIKNSKATLLLKGSRSLIAQRGHPVLVNSTGTPGMATGGQGDLLSGVIGAQLAAGLKPIEAATFGAWLCGRAAEIAIHSRSQSEQSLCPSDTANHLGHAFSDWQCSQR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be able to bind AMP ligand in a variety of conditions2. A protein with NADPHX epimerase activity, NADHX epimerase activity, ATP binding, ADP-dependent NAD(P)H-hydrate dehydratase activity, metal ion binding functions is required to enable nicotinamide nucleotide metabolic process.3. For general function, the protein need meet that Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP; Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.4. The designed protein must have at least one YjeF N-terminal domain. The designed protein sequence is ",
    "response#1": "MAAVDENAAWLGVPRKQLMESSGHAVARVVRDVADPGATVAIVAGRGNNGGDAFVAARFLDEYDVTTLLLGRAEAIGTEIARENWDALERADYDVRQVTDSRRFDLPDCDVVVDAMLGTGLSGDLREPAATAARTIDDADATVVSVDVPSGFDADDGDHADNGVEADHVVTFHDAKPGLEELGADLTVADIGIPAAAEEPALWQAHLPQLGAQSHKYTRGHALVVGGEQMTGASRLAARAAQRAGAGLVSMAVPPSVWPVYASALDSIMVAALDDTLTTDERIRAWLVGPGAGLGAETRRLTLALLATGRPCVLDADAISSFASQPQELWDALHEQCVLTPHAGEFARVFEEAPDRLEAARLAATQCGAVLVLKGADTVIAAPDGRALINACAPPWLATGGSGDVLAGLVCGLLTQGMPAFEAAGAAVWLHSQAALEFGPGLIPEDLIEVLPRVWQREPALWQAHLPQLGAQSHKYTRGHALVVGGEQMTGASRLAARAAQRAGAGLVSMAVPPSVWPVYASALDSIMVAALDDTLTTDERIRAWLVGPGAGLGAETRRLTLALLATGRPCVLDADAISSFASQPQELWDALHEQCVLTPHAGEFARVFEEAPDRLEAARLAATQCGAVLVLKGADTVIAAPDGRALINACAPPWLATGGSGDVLAGLVCGLLTQGMPAFEAAGAAVWLHSQAALEFGPGLIPEDLIEVLPRVWQRLQG",
    "response#2": "MQELDRQTIQEHGIPGLELMEHAGHACMEAIFHEFGYTGGKRAVIFAGRGNNGGDGYVIARLLLQSGWQVRVCILAERERIGGDAAINLEQLPPAAVSYCTVPGQLAGLLQEELSQTDVIVDALFGTGLNSEVAGINREAVELINAAGCPILAVDIPSGIHGTSGRVLGCAVRATVTVTFAAAKLGHVLYPGADHTGRLVVADIGIPAELVEGRPYVIGGGPYTGAPALAAQAALRAGAELSFVAAPASVAGEIQGYSEDLIVQPFESEHLTPSQVDDLVETAERYDDVVVLGPGLGTADETREAVRRFLESYGGPAVVDADALAVVPDVETDATLVCTPNRGELARMGGPEAESLAAAADEIESFAADLGHVVLAKGADDVITDGDRTRINRSGTPAMKVGGTGDLLSGIVAALLEHAEPFEAAAAGARVNGVAGERLDETKGIGTLASDMLDEIPPPRPAAQGHKYHRGHVLVLGGARLTGAARLCAQAAQRSGAGLVTVLAPQPVWPVYAQALQASMVQPFADAQQLGALLDDARAQVCAIGPGAGVQALTRQAVLAAAQRGKALVLDADALTVFAQQPQALFQALAANRAATAVLTPHEGEFARLFPDLAGSKPERAAQAARLAQAVVVLKGADTVIAAPDGRVLINSNAPPELATGGTGDVLAGMVAAWLAQGMPALAAACAAVWLHGQAAQRALGREGL",
    "response#3": "MKRLDQAAIKEYGVPGLVLMENAGRQVVEVIGDVLGIVRGKVVTVFIGKGNNGGDGLVIARHLLNMGAEVKVLALAGVEEITGDAAVNLEIWRKMGQKIYSLHHGDGINIVRLILMNSELIVDAIYGTGFRGKMEEKVGRIVEVLNGSGKPIIAVDIPSGLEANTGRVNEPCIQAAHTVTFALPKLGLILEPGADYAGELHVADISIPSVLLKYAHKGNFGHALLMVGSYGKIGAAILSSRACLRSGVGLLTVHVPKEGVEIIQTAVPEAMCSTSDEQNFITDIPLLDAYDAIGVGPGLGMEKQTQNALKLLIQQATVPLVLDADALNILSENKTWLAFLPSSSILTPHPKEFERLVGKWNNDEERLKLQIDFSAKNDVIVVLKGAHTSISTPLGKVYFNSSGNPGMATAGAGDVLTGIITSLLAQGYLPENAAIFGVYLHGVAGDVAVENTGEEALIASDILLAPGSNSHKYTRGHALVVGGEQMTGASRLAARAAQRAGAGLVSMAVPPSVWPVYASALDSIMVAALDDTLTTDERIRAWLVGPGAGLGAETRRLTLALLATGRPCVLDADAISSFASQPQELWDALHEQCVLTPHAGEFARVFEEAPDRLEAARLAATQCGAVLVLKGADTVIAAPDGRALINACAPPWLATGGSGDVLAGLVCGLLTQGMPAFEAAGAAVWLHSQAALEFGPGLIPEDLIEVLENKLRKLREGREVRHKYQNGNVYVLGGSPGLTGAVILTAKAAWSTGCGSVQVFTTPEVSPAYDAHFVDITRKMIPGDGSGLFKADAVRAVAEVIAARPGVLVLGPGLGTHPETAAAVRQLLRLVKAPVVLDADGVRALSGHTEKLPDDLRLIVTPHPGEQEQLLGFSIAQPEQLAQALNHFPCRDNMVFLAKGSPALLATRNECRITTYDTRIFSRTGFGDVLAGLMAGMLSRLPDSTVTAESMLDVSALAMLES"
  },
  {
    "reference": "MENQLWQSTLGCCSQYQESPQDAENILFLLLGLIILVNISINVTTVIWHGLQNAIDKMFSRMHQKTAEVQVTECPPKEPQPANVQDVHIHCVLDPVQVKMAQPTQCSSSSTHYFRKHSNDRRSRRRYCYPRGSLQIRQSNQQQSCHSRQQRLRNNRQFSHGYPPFRKQRQSHKASQTRPMPFFDLEDRDSLLEDDQSCPHPKQPRRSRGGLYKPVRLASNAGLWGRQGGILASLPLPSLYLSPEMRRLPKRVEAKSELRLQGFGPHYSQSRICGNVEAEQWASSPPPPRRLLPNPSWVTVGYSPFPSGGHIPYDARDQWRRGTEGCEPPPAFVSRNLRSDAQGYRDHSSSQAHRQNFPSYTHSQPNHSPPQSVGYSSRESHEVRRRAPDWSEAFPSRHPLTTSTSLTALGEASYQRAPTASSGLVIPHSSQRLAEGQISDPTPPATTFVPLSRNPGGNANYQVYDSLELKRQVQENRGRASSLPPPSTSASRPSLHRSRTGKLN",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should exhibit Polar residues, Basic and acidic residues compositional bias.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The designed protein should have a specific function within the membrane. The designed protein sequence is ",
    "response#1": "ILRPYEKGVVERLGKYQRTVESGLVVIIPFIEAIKKVDMREQVVDVPPQEVITKDNTVVVVDCVIFYEVVDPFNAVYNVVDFYQAITKLAQTNLRNIIGDLELDQTLTSREMINTQLREVLDEATDKWGTRVVRVEIQRIEPPGDIVEAMSKQMKAERMKRAAILEAEGR",
    "response#2": "ILRPYEKGVVERLGKYQRTVESGLVVIIPFIEAIKKVDMREQVVDVPPQEVITKDNTVVVVDCVIFYEVVDPFNAVYNVVDFYQAITKLAQTNLRNIIGDLELDQTLTSREMINTQLREVLDEATDKWGTRVVRVEIQRIEPPGDIVEAMSKQMKAERMKRAAILEAEGC",
    "response#3": "ILRPYEKGVVERLGKYQRTVESGLVVIIPFIEAIKKVDMREQVVDVPPQEVITKDNTVVVVDCVIFYEVVDPFNAVYNVVDFYQAITKLAQTNLRNIIGDLELDQTLTSREMINTQLREVLDEATDKWGTRVVRVEIQRIEPPGDIVEAMSKQMKAERMKRAAILEAEGQ"
  },
  {
    "reference": "MKIAVVGATGLVGHVMLQVLLERKFPFAEVLAVASEKSIGKKINFNNKQLEVIGMKQAISLKPDIALFSAGGSTSLQWAPEFAKVGTTVIDNSSAWRMHEHIKLVVPEVNTHVLSKTDKIIANPNCSTIQLVVALAPLHKKYTIKRVVVSTYQSVTGTGVKAVKQLENERKGIDVEKAYPHPIDLNCFPHGGTFEENGYTTEETKLVNETRKIIGDQSIQVTATVVRIPVYGGHSEAVNIEFENDFDLNDVKKLLSQSPGITLQDEPAKNIYPMPKYSEGKDDVFVGRIRRDFSQPKTLNMWIVSDNLRKGAATNAVQIAEYLVEKKLV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.2. The protein design should be able to enhance the efficiency of 'de novo' L-methionine biosynthetic process, diaminopimelate biosynthetic process, lysine biosynthetic process via diaminopimelate. The designed protein sequence is ",
    "response#1": "KPLHDAGKIRRVIVSTYQATSGAGLQGNIDLQEGTRAALDGADYEYKAFAHPIAFNAIPQIGSHKEQGYTSEEMKMVYETRKILGDETIMVCPTCVRIPVSNCHSESILVETEKPISVDEAKALFAATPGITVVDDLDASSYPMPSNSTSSDEVYIGRIRKDLSNPNGLAFWCVSDNLRR",
    "response#2": "LKPIYDAVGISRINVATYQAVSGTGKEAIHELVKQLTELLNGRPITSSVYPKQIAFNVLPHIDEFQTNGYTREEMKMVWETQKIMEDDSLMVNPTTVRVPVLYGHSEAIHLELKSPLTSDDARALLKKAPGVKVIDNPSKLEYPTPITHASGHDEVFVGRIRQDITHPTGLNMWVVADNIRR",
    "response#3": "KPLHDAGKIRRVIVSTYQATSGAGLQGNIDLQEGTRAALDGADYEYKAFAHPIAFNAIPQIGSHKEQGYTSEEMKMVYETRKILGDETIMVCPTCVRIPVSNCHSESILVETEKPISVDEAKALFAATPGITVVDDLDASSYPMPSNSTSSDEVYIGRIRKDLSNPNGLAFWCVSDNLRY"
  },
  {
    "reference": "MHWTEMYPEELKECMAEAGEKPFRGVQLFEGHHGKRKALEEMSNIPDRLAKKLGDAPYAVRIVKRLASKLDETKKYLYRLHDGEIVEGVLMRYHHGYSLCVSSQVGCKMGCRFCVSTRGGLIRNLRPYEMLDQVYAVEAREGISISNVILMGSGEPLDNYDHVLRFLRLLHDPKGKNMSYRNMTISTSGVVEGIDRLAEEDIPVNLAVSLHETDQSARKKIMPIAARYPLEELMRALRRYADRTGQRITFEYTLIRGKNDGERNIREMQSLLRGLPAHINLIPLNPTDHYDESRPAREEILRFQRELSQKGLSCTIRRELGSDIQASCGQLKAGCEAEETR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein contains novel Radical SAM core domains that confer a unique function or activity.2. The [4Fe-4S] cluster, S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions.3. The designed protein should be optimized for rRNA binding, tRNA (adenine-C2-)-methyltransferase activity activity in order to improve rRNA base methylation.4. The protein with the reaction adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine, the Proton acceptor must be optimized to ensure efficient catalysis.5. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.. The designed protein sequence is ",
    "response#1": "LLGMPADELRKLAGASGEKEFRGKQVADWIYRQGCREIDGMKNLPDAFELHVLRLERKEKLYGKTLDELIAVTRQIILPGYAARQIADWLYKKEITEIEEMSNLSKKTREALSEAYEIGLCPPVSEAVSSDGTKKYLFKVLNDKYIETAFIPEDDRATICLSCQAGCKMGCIFCLTGKQGFQGNLSSNEILNQFRSIPEFRKLTNIVYMGMGEPLDNLDEVIKSLEILTGLWGYGWSPTRITVSTIGLKNQVREFLERSRCHLAVSLHSPFDEERSRLMPIQKTNNIREILEIIRTADLNSQRRISFEYILFKGINDTPQHVQELARLLNGIKCRINIIRFHTIPGSEFTSPSLQEAEGFRDILNKKGILATVRRSRGEDIGAACGLLSTRAMNKQR",
    "response#2": "ARGAIADWLAARGEPAYRLRQIVPRMWERPAASWADATDLPAALRGALEQAFPIARLELAAHQVSRDGTEKFLWKLADGEAIESVLIPEGKRRTLCISSQVGCALGCVFCATGQMGWRRNLSPAEIAGQVRETVIREPALKPTNVVFMGMGEPLLNWEHVDAALTILNHPLGLGIGARHITVSTVGILPNLAKLARRPEQFRLALSLHAPTPAQRLALMPIERKYTLADVLAALRRFRRRVTLEYVLIGGQNDSLETADALAAIARPLGALVNLLPLHPGGAPGLTPSTHAQMLAFERRLKHHGVEAVLRRSRGLDISAACGQLRVELG",
    "response#3": "KKLLLGMPADELRKLAGASGEKEFRGKQVADWIYRQGCREIDGMKNLPDAFKISLLGGYEVGRSETVKINRSKDGTFKLLLMPAYGELIETVGMPYSDRFSCCVSTQVGCSIGCAFCATGACGFKRNLAVGEIIDQVLTVQEIALREKLLPAGGRVSHVVFMGMGEPLINYDATLAAVKLLNTELNIGMRNITVSTIGYVPGIYKLSKEKLQITLAVSLHASEDSLRRRLVPGMSRYKLEEIVEACRAYFDETGRRVSFEYCLLKGINDSKDDAARLAVLIRGLNCHVNLIPYNAVEGCVYRSPEQARVADFRQVLEDASIVVTQREQRGSGIEAACGQLRRKRVE"
  },
  {
    "reference": "MDIETKKVIEQILDNNKIVLFMKGSKLMPMCGFSNTAIQILNTLNTDYFTYDILENENIRQAIKEHSSWPTIPQLYINREFVGGADIMLELFEQGELQAQVETLLAA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein can be expressed in chloroplast and perform metal ion binding without triggering undesirable cellular responses.2. The protein should be able to bind [2Fe-2S] cluster ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIETPTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQLRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIATHPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTLPPPLL",
    "response#2": "MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIETPTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQLRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIATHPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTLPPPLL",
    "response#3": "MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIETPTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQLRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIATHPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTLPPPLV"
  },
  {
    "reference": "MIQPDPTVLALALLLDLCLGDPRWLPHPVVMIGRLITFLETLLRRCMANERIAGVLLLALTVTSAASVTWLMVWGSARLHALAGLMVAALLSSTCLAARSLQRESCLVADALDAGDIASARVKLSYIVGRDTVDLDEEEIWRALIETVAENTTDGIIAPLFWLALGGPVAGMAFKAVSTLDSMVGYKNERYLRLGWASARMDDLVNYIPARLTALLMVMVAPLIGLSQANAASIALRDRLNHPSPNSAHPESAAAGALGIRLGGPSTYGGLLSVKQFIGDPLRSIDGQAYRGMIRLMYATTLAMAVISLATAALLRGIHVTQL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that contributes to cobalamin biosynthetic process by functioning in plasma membrane with threonine-phosphate decarboxylase activity, ABC-type vitamin B12 transporter activity.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The protein must exhibit the following characteristics: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group..4. The designed protein should interact with enzymes and substrates within the Cofactor biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "NVVVPVLTRISNHTDFDVLRLNPDINLRYVGKGEKIDKADLVILPGTKSVRDDLDYLRSQGWDKDIQRHIRLGGKVIGICGGYQMLGKLIDDPNGVEGSPGKSEGLGLLDITTTLTDSKQLTNTHAQLCLNGKTANVKGYEIHVGRSEVQGAQPLRLSSGEAEGAISECGQIMGTYLHGFFDEETHQHALLDVLKNVVVPVLTRISNHTDFDVLRLNPDINLRYVGKGEKIDKADLVILPGTKSVRDDLDYLRSQGWDKDIQRHIRLGGKVIGICGGYQMLGKLIDDPNGVEGSPGKSEGLGLLDITTTLTDSKQLTNTHAQLCLNGKTANVKGYEIHVGRSEVQGAQPLRLSSGEAEGAISECGQIMGTYLHGFFDEDETHQHALLDVLK",
    "response#2": "MVQGTTSDAGKSVLVAGLCRVLARKGIKVAPFKPQNMALNSAVTKDGGEIGRAQAVQAQACNVEPTVHMNPVLIKPNSDTGAQIILQGKALSNMDAVGFHDYKRVAMGTVLDSFAKLTDEYESVMIEGAGSPAEINLRENDIANMGFAEEADVPVIIIADIDRGGVFAHLYGTLALLSESEQARVKGFVINRFRGDIGLLESGLDWLEEKTGKPVLGVLTF",
    "response#3": "MVQGTTSDAGKSVLVAGLCRVLARKGIKVAPFKPQNMALNSAVTKDGGEIGRAQAVQAQACNVEPTVHMNPVLIKPNSDTGAQIILQGKALSNMDAVGFHDYKRVAMGTVLDSFAKLTDEYESVMIEGAGSPAEINLRENDIANMGFAEEADVPVIIIADIDRGGVFAHLYGTLALLSESEQARVKGFVINRFRGDIGLLESGLDWLEEKTGKPVLGVLNVVVPVLTRISNHTDFDVLRLNPDINLRYVGKGEKIDKADLVILPGTKSVRDDLDYLRSQGWDKDIQRHIRLGGKVIGICGGYQMLGKLIDDPNGVEGSPGKSEGLGLLDITTTLTDSKQLTNTHAQLCLNGKTANVKGYEIHVGRSEVQGAQPLRLSSGEAEGAISECGQIMGTYLHGFFDEDAAAA"
  },
  {
    "reference": "MLRTYQPKKRHAKKEHGFRKRMSTSNGRKVLRRRRLKGRKVLSA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have structural constituent of ribosome within ribosome to regulate translation. The designed protein sequence is ",
    "response#1": "MPLQLQLRTDHRRSYVKQMRKQGHIPAVLYGKTTNSQPVFVDAAEFLKQLRATGGTGLLDAKMNDNHIRIIVRDMQKHPIRHDIIHIDFLK",
    "response#2": "EIDVHVPIHLVGEAAGVKDGGVLQQAMHQLSIRVLPSNIPPSIDVDITNLQVGDVITVSHIQTNGAYEINHDPTEVIASILPPL",
    "response#3": "MPLQLQLRTDHRRSYVKQMRKQGHIPAVLYGKTTNSQPVFVDAAEFLKQLRATGGTGLLDAKMNDNHIRIIVRDMQKHPIRHDIIHIDFLK"
  },
  {
    "reference": "MSRINKEEVKKVAQLARLELNINEINNHAEQLEKILDYIRQLEKIDTDNVPCTTRAIEVINVFRKDEKKNYDRTEELLELGPSREDKYFKVPKIMNE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein have enhanced ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+)   + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate.2. The designed protein should have glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity, asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity, ATP binding to facilitate regulation of translational fidelity, translation.3. For general function, the protein need meet that The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp- tRNA(Asn) or phospho-Glu-tRNA(Gln). The designed protein sequence is ",
    "response#1": "VIGLEVHVELNTKSKLFSSAQNRFGDEPNTNISPVCTGMPGSLPVLNKEAVNKAILFACAVQGEVALLSRFDRKSYFYPDSPRNFQITQFEHPIVRGGYVKAIVRGEERYFELAQAHIEDDAGMLKHFGEFAGVDYNRAGVPLIEIVSKPCMFCADDAVAYATALVSLLDYIGISDCNMEEGSVRFDVNVSVRPKGSEELRNKVEIKNMNSFAFMAQALEAERCRQIEAYLENPNKDPKTVIPGATYRWDPEKKKTVLMRLKERAEDYKYFIEPDLPVLQLTEAYIEEVKKSLPEMPDARKARYVKEFGLPEYDAGVLTSTKELAEWFEEAAKKSSSPKKCANWILAEVLAILNETNSSLSDLKFGPEAIAELVNVIEEGKITSKQAKDVFAEMIACGKKPSAVIAEKGMEQVSDSSFIEKIVEEVFAENAEAVQDWKNGKTNVAGWLMGQVMKKSGGKANPKQAADLVNKRLG",
    "response#2": "VIGLEIHLQLNTNSKLFCSCPSGMADNPIPNSAVCPVCTAQPGTLPVLNKGAVELAVKAALALNLEINKISLFDRKNYFYPDLPKGYQITQLFKPISEHGWLEVNGRKVGITRAHMEEDAGKSIHHASYSLIDWNRAGTPLLEIVSEPEIHSADEAYEFLTKLKSNVSWVGASNADMEKGELRVDVNISLRPAGIKTFGTKVEIKNLNSFKAVRDAINVEIERQTEMLNEGKKIKQETLLFDKEKGETVSMRSKEDALDYRYFPDPDLPPLVLADEWLAEETEHYADASWNFFELATKECKNYRALSNWVTVEFAGRCKTQGKNLAFSGILPSGVAQLVNFIDKGVITGKIAKDIADMMMESPDKSPEVILKENPEMLPMTDESALVAIISEVISANPQSVVDYKSGKTKALGFLVGQIMKRTQGKAPPNRVNEILLVKS",
    "response#3": "VIGLEIHLQLNTNSKLFCSCPSGMADNPIPNSAVCPVCTAQPGTLPVLNKGAVELAVKAALALNLEINKISLFDRKNYFYPDLPKGYQITQLFKPISEHGWLEVNGRKVGITRAHMEEDAGKSIHHASYSLIDWNRAGTPLLEIVSEPEIHSADEAYEFLTKLKSNVSWVGASNADMEKGELRVDVNISLRPAGIKTFGTKVEIKNLNSFKAVRDAINVEIERQTEMLNEGKKIKQETLLFDKEKGETVSMRSKEDALDYRYFPDPDLPPLVLADEWLAEKVKSVPAAKNFFELATKECKNYRALSNWVTVEFAGRCKTQGKNLAFSGILPSGVAQLVNFIDKGVITGKIAKDIADMMMESPDKSPEVILKENPEMLPMTDESALVAIISEVISANPQSVVDYKSGKTKALGFLVGQIMKRTQGKAPPNRVNEILLVES"
  },
  {
    "reference": "MRLGSLVEELDVEVAKAVNAAGDLDTSVTGFAIDNRKVAPGTVFGAFQGLRVNGEDYIPEAIRSGAIAIVARPEAKVDGALHIADPNPRRLFAQLAARFFAPFPDVTVAVTGTNGKTSTVELARQLWRMLGHKSASIGTLGVTTAVDQVSTGLTTPDIVTFLANMAGLRREGITHAAFEASSHGLDQYRTEGLPVLAGAFTNLSRDHLDYHGDMDSYFDAKMRLFDEVVAPQGSAVIWADDAWSDKAIERATKRGLRVLSVGVKGQGLRLANRTPTQLGQALEIEADGTLYKVNLPLIGAYQAANALTAAGLVIATGGDITRVMELLGRVQPVRGRLERAVISKAGAPVYVDYAHTPDGLRAAIEALRPHTRGRLITLFGAGGDRDAGKRPQMGKVAADLSDHVIVTDDNPRSEEPSAIRAAVLAGAPDAQEIGGRRDAIAAAIAQAGAQDIVLLAGKGHEQGQIIGDRVLPFDDVTVARECAG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be optimized for ATP binding. The designed protein sequence is ",
    "response#1": "VGPGELILYPRRRKESHKGENGIVAIIGGGAYTGAPYLAGMAALRAGCDLVYVCCPSSVWSVVASFSPDLIVRKISGEFLTVEGLKEVEDIIEKADAIVVGPGLGKRKEVLEACKFIFENYDKKFVYDADAIEVLKGKKCNQKVIITPHSGEFKRLTGIDLPDDIEERSRIVKKEAKKLEATILAKGAIDIISDGKRLKMNKIHNEGMTVGGTGDTLAGICAAMLAKKIAPFYSACISAFINGMAGNMAYEEKGNIVATDMIEKIPLVIKKI",
    "response#2": "GPGELILYPRRRKESHKGENGIVAIIGGGAYTGAPYLAGMAALRAGCDLVYVCCPSSVWSVVASFSPDLIVRKISGEFLTVEGLKEVEDIIEKADAIVVGPGLGKRKEVLEACKFIFENYDKKFVYDADAIEVLKGKKCNQKVIITPHSGEFKRLTGIDLPDDIEERSRIVKKEAKKLEATILAKGAIDIISDGKRLKMNKIHNEGMTVGGTGDTLAGICAAMLAKKIAPFYSACISAFINGMAGNMAYEEKGNIVATDMIEKIPLVIK",
    "response#3": "VGPGELILYPRRRKESHKGENGIVAIIGGGAYTGAPYLAGMAALRAGCDLVYVCCPSSVWSVVASFSPDLIVRKISGEFLTVEGLKEVEDIIEKADAIVVGPGLGKRKEVLEACKFIFENYDKKFVYDADAIEVLKGKKCNQKVIITPHSGEFKRLTGIDLPDDIEERSRIVKKEAKKLEATILAKGAIDIISDGKRLKMNKIHNEGMTVGGTGDTLAGICAAMLAKKIAPFYSACISAFINGMAGNMAYEEKGNIVATDMIEKIPLVIKKFQ"
  },
  {
    "reference": "MTARLLLASRNAKKLRELRQVVADAGIVGLEVVGLDEVEPFEELPEDAPSFEGNALIKARQGYERTGLPCLADDSGICVDALNGMPGVLSARWSGRHGDDPANTALLLAQLSDVPAERRGGEFVSACALVSDSGESTVRGVWRGTILREPRGDGGFGYDPVFLPEESTVTAAEMPPEQKNAVSHRARALSSLVDPLRELAARVPE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must possess metal ion binding, dITP diphosphatase activity to support purine nucleoside triphosphate catabolic process, nucleotide metabolic process under certain conditions.2. The protein should have a high affinity for Mg(2+), substrate.3. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.4. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "MITFLLPLVIALFL",
    "response#2": "MSILSGR",
    "response#3": "MNIILANPRGFCAGVDRAISIVERALELYGAPIYVRHEVVHNRFVVEDLRNRGAIFVEELHEVPDDTAILSAHGVSKAVRTEARSRGLNVLFDATCPLVTKVHMEVAKASREGREAILIGHAGHPEVEGTMGQYNNAEGGMYLVESPADVWKLEVKDEDNLCFMTQTTLSVDDTSEVIDALRKRFPKIVGPRKDDICYATQNRQDAVKDLASECDVLLVVGSPNSSNSNRLAELAQRVGKPSFLVDNAEKICEDWLSGVTNIGVTAGASAPEILVQQVIDRLKEFGYEAVTELDGREENIVFEVPKELRVEVK"
  },
  {
    "reference": "MTKKDIRSLKPAELQTILSDLGQPSFRIRQIEEWVWKRSCKDFNEMSNLPAALRTQLAEMFSIHGIVVDDMQRSIDGTIKNAVKLHDGRVVESVLIPTDKRITACISSQVGCSLDCHFCATARLKRMRNLNAEEIYDQVVNIKNQAEQYFNRPLTNIVYMGMGEPLLNYQNVLGSIDKICGDSGLGMAPRRITVSTVGIAKMIRSLADDNPRTNLALSLHSARNEVRESIMPITKTNPIEMLRDAMNYWYSKTSSKVTFEYVIWKGINDQIEDIKALVKLCKQVPSKVNIIEYNPIGDDRFRMAEAEVIKAYQDALGEIGVVAKVRQSRGSDIDAACGQLANKNLANLQV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must be engineered to have a high affinity for [4Fe-4S] cluster in order to increase its catalytic activity.2. The protein design must be able to perform tRNA binding in order to contribute to rRNA base methylation.3. The protein contains novel Radical SAM core domains that confer a unique function or activity.4. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. The designed protein sequence is ",
    "response#1": "MTACVSSQVGCSLTCTFCATGKMDRIRNLDPAEIYDQVVAIHNQAEKYFDNPLTNIVYMGMGEPLLNYANVLKSIEYITSPEGLGMSPRRITVSTAGIAKMIKKLGDDEVKFNLALSLHAANDVKRNQIMPINESNTLEALREALKYYFQKTKNPVTYEYIAFNKFNDSLKDAEELYQFCKHLPCKVNIIEYNPIEDAEFTKSEGDRLDKFSEYLRK",
    "response#2": "SSQAGCAVGCRFCSTGHQGFSRNLSTGEILAQLWFAEHFLRAHLETHGERVISNVVMMGMGEPLQNYAALVPALRVMLDDHGYGLSRRRVTVSTSGVVPMIDRLGEDCPVALAVSLHAPNDELRDKLVPLNRKYPIAELLDACNRYLEHAPRDFITFEYCMLDGVNDQPEHR",
    "response#3": "SSQAGCAVGCRFCSTGHQGFSRNLSTGEILAQLWFAEHFLRAHLETHGERVISNVVMMGMGEPLQNYAALVPALRVMLDDHGYGLSRRRVTVSTSGVVPMIDRLGEDCPVALAVSLHAPNDELRDKLVPLNRKYPIAELLDACNRYLEHAPRDFITFEYCMLDGVNDQPEHRD"
  },
  {
    "reference": "MMPLKPQHWKLLAALSDGLPHHISALGLAAGVKPQQINGLWQQMPPHIRGLLRQHDGQWRLVRPLAVFDEAQLQQTGRQHGFQTALYHECTSSNDIVLEAARVSPQQAHGLLVLAHSQSKGRGRQGKVWQNRLGECLMFSFGSVFDKPQNELGALAPAVALACHAALAKLGIPAQIKWPNDLVVGRRKLGGILIETVRSSGKTVAVIGIGINFVLPKEVEDAASVQAAVSAAAPPVGSAALLAALLGELDTMLNRYRQYGFAPFVAAYQAADRDHNRPVRLLQNGETVYEGVSAGITGQGALRLQTENGERIVVSGEISLRPGDNPPPVQTAAAGKYLLLDGGNSQLKWAWVENGSIVHTGRSPYRDLTLLGTEWQERGSGSVRIIGSAVCGEAKKAQVAAQLPQPVEWLSSMPQALGIRNHYRNHTEHGADRWFNALGSRRFTQNACVVASCGTAVTVDALTDNNHYLGGTIMPGFHLMKEAMALKTANLNRPLGRVYPFPTTTANALASGMMDAVCGSVLLMHARLREKVGAGKAVDLIITGGGAAKVVQALPPSFVLDNTVKIVDNLAIYGLLNWIQQQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The ideal protein would be one that plays a critical role in phosphorylation through biotin-[acetyl-CoA-carboxylase] ligase activity, pantothenate kinase activity within cytoplasm.2. The protein must bind to K(+) in order to perform its enzymatic function.3. For general function, the protein need meet that Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis.4. A protein should be able to manipulate the activity of enzymes in the CoA from (R)- pantothenate: step 1/5 pathway, either by activating or inhibiting their function.5. The designed protein must have at least one BPL/LPL catalytic domain.6. The protein with the reaction ATP + biotin + L-lysyl-[protein] = AMP + diphosphate + H(+) +   N(6)-biotinyl-L-lysyl-[protein], the Proton acceptor must be optimized to ensure efficient catalysis.7. The designed protein should have a high specificity for ATP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MAPIEHIHIPEIDSTSTEVKRQLDQGKMPPFLVSSTSQSAGRGRTGRTWTSPAGNIYLSLALSQERIPKQDHGLLPLKTAYLVAAWVWQTFQLRLTIKWPNDLLFGARKLGGILCESQSDGTSWGPFIVGVGLNLETPPVVDQGTTSLGEHCTLDGIVVGDAARRLGQYLLDGLCQPGPGSVLVRRDEEQVRQIGAEHGFQTALKHECSSSNDIVLEQAKQSVESAHRFLCVTHHQKKGRGRQGKAWEDRLGECLMFSFGWAFDKAQGELGALALVAALACQRALAAFGVSAQIKWPNDLVVGRQKLGGILIETVRSSGKTVAVIGIGINFVLPKEVENATSVQAAFQTASRQGLAVARLLSLLLKELGGLLAQFDEEQVRQIGAEHGFQTALKHECSSSNDIVLEQAKQSVESAHRFLCVTHHQKKGRGRQGKAWEDRLGECLMFSFGWAFDKAQGELGALALVAALACQRALAAFGVSAQIKWPNDLVVGRQKLGGILIETVRSSGKTVAVIGIGINFVLPKEVENATSVQAAFQTASRQGLAVARLLSLLLKELGGLLAQFDEEQVRQIGAEHGFQTALKHECSSSNDIVLEQAKQSVESAHRFLCVTHHQKKGRGRQGKAWEDRLGECLMFSFGWAFDKAQGELGALALVAALACQRALAAFGVSAQIKWPNDLVVGRQKLGGILIETVRSSGKTVAVIGIGINFVLPKEVENATSVQAAFQTASRQGLAVARLLSLLLKELGGLLAQF",
    "response#2": "MAPIEHIHIPEIDSTSTEVKRQLDQGKMPPFLVSSTSQSAGRGRTGRTWTSPAGNIYLSLALSQERIPKQDHGLLPLKTAYLVAAWVWQTFQLRLTIKWPNDLLFGARKLGGILCESQSDGTSWGPFIVGVGLNLETPPVVDQGTTSLGEHCTLDGIVVGDAARRLGQYLLDGLCQPEQLFVESSQTTSTNTILLEQVRHLPADTPPQICLAYEQTAGRGRQDKAWKNRIGECLMFSLSWSFLRPQAELGGLALVVALAVAQTLRELGVAAQIKWPNDLMLDNSKLGGILIETIPRQKQQTTAVIGIGLDEEQVRQIGAEHGFQTALKHECSSSNDIVLEQAKQSVESAHRFLCVTHHQKKGRGRQGKAWEDRLGECLMFSFGWAFDKAQGELGALALVAALACQRALAAFGVSAQIKWPNDLVVGRQKLGGILIETVRSSGKTVAVIGIGINFVLPKEVENATSVQAAFQTASRQGLAVARLLSLLLKELGGLLAQFDEEQVRQIGAEHGFQTALKHECSSSNDIVLEQAKQSVESAHRFLCVTHHQKKGRGRQGKAWEDRLGECLMFSFGWAFDKAQGELGALALVAALACQRALAAFGVSAQIKWPNDLVVGRQKLGGILIETVRSSGKTVAVIGIGINFVLPKEVENATSVQAAFQTASRQGLAVARLLSLLLKELGGLLAQFAGAQLKLPARRIVFVHHQTQGRGRQGRSWQSRIGECLMFSAGWRFARAQAELGGLTLVAALVCCRALRGLGVPAQIKWPNDLVIGGEKLGGILTETARRNSQTAIVVGIGI",
    "response#3": "MAPIEHIHIPEIDSTSTEVKRQLDQGKMPPFLVSSTSQSAGRGRTGRTWTSPAGNIYLSLALSQERIPKQDHGLLPLKTAYLVAAWVWQTFQLRLTIKWPNDLLFGARKLGGILCESQSDGTSWGPFIVGVGLNLETPPVVDQGTTSLGEHCTLDGIVVGDAARRLGQYLLDGLCQPSWGWGMDDEEQVRQIGAEHGFQTALKHECSSSNDIVLEQAKQSVESAHRFLCVTHHQKKGRGRQGKAWEDRLGECLMFSFGWAFDKAQGELGALALVAALACQRALAAFGVSAQIKWPNDLVVGRQKLGGILIETVRSSGKTVAVIGIGINFVLPKEVENATSVQAAFQTASRQGLAVARLLSLLLKELGGLLAQFEGKVLPPMPFDRPLAVLPETQEHDFFDIRVLSCTTSTNDELLQRARDGLPIHRQVVVAHEQSAGRGRQGRVWVHRLGECLMFSVGWSFMQEQAQLGALALVVALSCQRSLQALGCAVQIKWSNDLVVGRDKLGGILIESLRQNHQTHTVIGVGINFVVPKNLAYTTSLQAACSRKMTAGQVLNQILDALHEDLNLFDEEQVRQIGAEHGFQTALKHECSSSNDIVLEQAKQSVESAHRFLCVTHHQKKGRGRQGKAWEDRLGECLMFSFGWAFDKAQGELGALALVAALACQRALAAFGVSAQIKWPNDLVVGRQKLGGILIETVRSSGKTVAVIGIGINFVLPKEVENATSVQAAFQTASRQGLAVARLLSLLLKELGGLLAQF"
  },
  {
    "reference": "MGLSTIPEAIKDFRAGKFVIIVDDEDRENEGDLAVAAEKVTPDAINFMALHARGLICLPIVGERLDELRIPLMVMDNDTKFCTAFTVSIEAKHGISTGISATDRAQTIKTVIDPATKADDLVRPGHIFPLRARGGGVMVRAGHTEAVVDLARLAGLYPAGVICEIMNEDGSMARLPQLEVMAEKYGIKVVSVADLIAYRRRHEKLVHRVAEAKMPTKYGKFRAIGYKSDIDTDEQLALVMGDISTEEPVLVRVHSECVTGDVFGSLRCDCGEQIALAMQTIANEGRGVLLYMRQEGRGIGFHNKIRAYALQDEGLDTVEANLCLGFAPDLRDYGIGAQILVDLGLHDIRLLTNNPKKVVGLEGYGLKVVETVPLIVSPTPYNQDYLKTKQKKLGHLLEIEGATDN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be designed to catalyze the following reaction GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.2. The designed protein should be optimized for 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, manganese ion binding, GTP binding, magnesium ion binding activity in order to improve riboflavin biosynthetic process.3. The protein design should take into account the physiological conditions of the Cofactor biosynthesis pathway.4. For general function, the protein need meet that Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate. The designed protein sequence is ",
    "response#1": "VEVDMPTEYGHFKLVAYEQTNSGDVHLALVKGSWEKDEPVLVRVHSSCVTGDIFGSCRCDCGPQLHAAMKMVDQAGKGVVLYMKQEGRGIGLMNKLKAYKLQEGGLDTVEANIKLGFDMDERDYGVGAQILRDLGISKIRLITNNPKKRVGLMGYGLEIVENLVTE",
    "response#2": "VRVDMPTQYGHFKLVAFQEKNSSNEHLALIKGSWQPEEPVMVRMHSSCFTGDILASLRCDCGEQLHQAMQMVEAEGKGAIIYMNQEGRGIGLLNKLKAYRLQEQGMDTVEANLHLGFQTDQRDYGVGAQILRHLGITKLRLISNNPKKRVGLIGYGLEIVENLIGVHEPERVHLPTEFGEFEFIPFRQLSNDLEHAALVKGTWTKDETVLVRIHSSCLTGDIFGSLRCDCGAQLHKAMQMVEEAGKGAVIYLSQEGRGIGLFNKMRTYKLQEQGMDTVEANVELGFGEDERDYGIGANIMRELGLGKIKLISNNPVKRAGLEGYGIEIVETDMRW",
    "response#3": "VRVDMPTQYGHFKLVAFQEKNSSNEHLALIKGSWQPEEPVMVRMHSSCFTGDILASLRCDCGEQLHQAMQMVEAEGKGAIIYMNQEGRGIGLLNKLKAYRLQEQGMDTVEANLHLGFQTDQRDYGVGAQILRHLGITKLRLISNNPKKRVGLIGYGLEIVENLEEFAPIVEVDMPTEYGHFKLVAYEQTNSGDVHLALVKGSWEKDEPVLVRVHSSCVTGDIFGSCRCDCGPQLHAAMKMVDQAGKGVVLYMKQEGRGIGLMNKLKAYKLQEGGLDTVEANIKLGFDMDERDYGVGAQILRDLGISKIRLITNNPKKRVGLMGYGLEIVENLEQYA"
  },
  {
    "reference": "MRVIFTGGGTGGHIYPIMAIIERLKERGISTNDKILFVGTKKGLESKIVPAAGVNFKTINIQGFNRKHPLKNFETIKLFLQATKSARKILKEFKPDVVLGTGGYVSGAMVYEAAKMHIPTMIHESNSVVGLANKFLGHYVDRICYTFDDAAKEFPEKKKLVKTGNPRSQQVLSLHEEKIDIKKKWGLNPDMPTVLVFGGSRGALAINRIMLKSLMNLKTKPYQIIWATGTYYFDSVQKKLKGVDIGTNIKVLPYIKNMPGLLPEMTCVVSRSGATSIAEFTALGVPVILIPSPNVTHNHQMKNALDLEKAGAALVIPEDDLNPNNFVSSIDHILLDEKYAKEMSQASKALGVPDASDQVIKVMEEISR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that can perform undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity in plasma membrane to support cell division, regulation of cell shape.2. The protein must exhibit the following characteristics: Cell wall formation.3. The UDP-N-acetyl-alpha-D-glucosamine binding site should be stable and able to withstand changes in environmental conditions.4. A protein that is capable of catalyzing reactions within the peptidoglycan biosynthesis pathway with high efficiency and specificity.5. The protein have enhanced Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-   cis-undecaprenyl diphosphate + UDP-N-acetyl-alpha-D-glucosamine =   beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-   di-trans,octa-cis-undecaprenyl diphosphate + H(+) + UDP. The designed protein sequence is ",
    "response#1": "MILVMAGGTGGHIFPGLAVAEALRERGWRVHWLGGRGNAGHPSMESQLVPPRGFAFESIDFSGVRGKGPLTLVFLPLRLLKAFWQSIQVVRRVKPDVVVGLGGYIAFPAGMMSVLLGKPLVLHEQNSVAGMVNKVLAGVADRVFGHPSAPADRLVAVGNPVRPDLVAAPLPEPGEREPLRVLLVFGGSQGAVSLNRAVAGAAEDLAASGVAVLHAYGLKNTLELRTPEYGEPPYVAVPYLDRMDLAYAAADLVICRSGAMTVAEVSAVGLPAIYVPFPIGNGEQRLNALPVVNAGGGLVVADADLTPGLVARQVVRLFSDPAQLAAMTAAAARVGHRDAAHRAAAALVADAAGRRGAGRMAELVAAVREFAGADPAAAE",
    "response#2": "VMILAGGTGGHIFPGLAVAKVLRARGVPVTWLGADGAMETRLVPQHAIQIDTLAISGLRGKGIVKLLGAPVRVMRAVRAAGFVLRKRQPRAVISFGGFAAGPGGLAARLLGVPLLVHEQNRAPGMTNKVLSRFARRVLCAFPGAFPEARLSGNPVRAEIAALPREPARFAAREGLPDGPVLLVFGGSQGAVSLNRAVAGAAEDLAASGVAVLHAYGLKNTLELRTPEYGEPPYVAVPYLDRMDLAYAAADLVICRSGAMTVAEVSAVGLPAIYVPFPIGNGEQRLNALPVVNAGGGLVVADADLTPGLVARQVVRLFSDPAQLAAMTAAAARVGHRDAAHAVADVVAGLASER",
    "response#3": "MKLVLFAGGGTGGHLYPGVAMASELKKVVPGVEISFAGTPAGIEATEVPRLGYPLHLLPVRGLKRGRSLRDLAANVGVLKDFGSSLMQAFSIIRKETPNVVVGTGGYVSAPLLLAAQLSGCKTLIQEQNAFPGVTTRMLARMASEVFVAYPGSFPEAEITGNPVRRSILQLPAPVARHSAPELLVLGGSQGARALNNAVPAALAALGHPAVDVRHQCGEKLRAEAEAAYAQAAVNASVEPFIADMAAAYAWADLVVCRAGASTLAEVCAAGVGSVLVPFAAAVDDHQTRNAEYLVSAEAAVLLKQDDTLAVRLQQVLQTLLADPARRLAMAQAARTLAKPDAAERIADIVLGEAGSHS"
  },
  {
    "reference": "MRETMQLVPMVVEQSSRGERSFDIYSRLLRERIIFLNGEVDDTVSALVCAQLLFLEAENPKKPIHLYINSPGGMVTSGFAMYDTMRYIRAPVHTLCMGTARSMGSFLLMAGEPGTRAALPNASILIHQPSGGFQGQASDMLIHAEEIRQTKHRMTRLYAEHCGRSYEEFETAMDRDRFMTVQEALEWGLIDRILEVREDAAA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Nucleophile active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.2. The protein must exhibit the following characteristics: Plays a major role in the degradation of misfolded proteins.; Cleaves peptides in various proteins in a process that requires ATP hydrolysis. The designed protein sequence is ",
    "response#1": "TESYKSLNEALVHGGIANDTHVNLKFVNSTDLQEEQGDISKYFEDIHGVLVPGGFGVRGTEGKILAIKYARENGIPLFGICLGMQLAVVEFARHVCNMENANSTEFDADTPWPVIDLLPGQISIERKGGTMRLGAYKCVLTPNTRAMEAYKSNVISERHRHRYEFNNKYEETLVEHGLVVSGKNPETSLVEIVELKDHPWFLGCQFHPEFKSKPMNPHPLFREFINRYTPSELRAHFKKLNWSRVVAFQTRNPMHRAHRELTVRAARDRQCNVLIHPVVGMTKPGDIDHYTRVREWHPLLLPLAMRMAGPREALWHAIIRKNYGCTHFIVGRDHAGPGKNSKGVDFYGPYDAQDLVAKYKDELHIEVVPFQMVAYVPDTDEYIPQDELPAGTKTLNISGTELRRRLKTGAAIPDWFTYPDVQTILREG",
    "response#2": "VNIGMVGKYVELPDAYKSVNEALKHAGLKKRLTVNIHYVDAQDVMVKGPHILKDLDAILVPGGFGERGTEGKMLAAQYARENKVPYLGICLGMQLALIEFARNVAGLTDAHSTEFNPDSPHPVVGLITEWLDAEGQVEMRNAKSDLGGTMRLGAQKCYLKPKSKAREVYGSEWIVERHRHRYEVNNNYIEQLEKAGLVFGGLSEDKKLVEMIELPDHPFYVAGQFHPEFTSTPRDGHPLFEGFVAR",
    "response#3": "LPDAYKSVNEALKHAGLKKRLTVNIHYVDAQDVMVKGPHILKDLDAILVPGGFGERGTEGKMLAAQYARENKVPYLGICLGMQLALIEFARNVAGLTDAHSTEFNPDSPHPVVGLITEWLDAEGQVEMRNAKSDLGGTMRLGAQKCYLKPKSKAREVYGSEWIVERHRHRYEVNNNYIEQLEKAGLVFGGLSEDKKLVEMIELPDHPFYVAGQFHPEFTSTPRDGHPLFEGFIR"
  },
  {
    "reference": "MAIKLEDKKAIVAEVNEAAKVALSAVVADARGVTVSAMTGLRKEAREAGVYVRVVRNTLLKRAVEGTEFSILNDAFKGPTLIAFSNEHPGAAARLFKEFAKGQDKFEIKAAAFDGNFIAANQIDVLATLPTRDEAIARLMSVIQGATSKLARTLAAIRDQKEATAA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors..2. The designed protein must possess structural constituent of ribosome, large ribosomal subunit rRNA binding to support translation under certain conditions. The designed protein sequence is ",
    "response#1": "MVTPPTASLIMKELGIQKGSGNAGSEVVGNISISQVAKIARMKKEDVLSYDLKATMKEVMGTCVPMGVTI",
    "response#2": "MI",
    "response#3": "TPPTASLIMKELGIQKGSGNAGSEVVGNISISQVAKIARMKKEDVLSYDLKATMKEVMGTCVPMGVTV"
  },
  {
    "reference": "MVFKPLTIAAAIAGLTPFVSAFDAQAKSNVAVYYGQGYGQQRLSHFCQETSLDIINIGFINTFPDQGAAGWPGSNFGNQCDGLTYEVDGVSTKLLSGCHQIAEDIPICQAAGKKVLLSLGGASPDNQQILSDASAVRFADFLWGAFGPQTEEWVSNDGPRPFGEVVVDGFDFDIEHNGGFGYATMVNRFRELFALVPERTFYISGAPQCPIPDPQLSDAIAQSPFDFVWVQFYNTAGCAASDSINGVSTGFNFDDWVDVIKRGANPDAKLYVGLPAGPGAAGAGYYLTPEEVYPLVDVYMNLYPETFGGIMLWEATASDENTFSGLTFADVIKDILVAVDPSPPVPSPSSSSVIASSTPVASSTPVASSAPASSTPISSGSPVPSSSAVSSSPAVSSTTESSSTQVVSGSVSASSSPITSSPVASSTPVASSAPSATSSAVASSSPIAPSSPVASSSAIASSSAIASSSAIASSSAIASSSAIASSSAIASSSAIASSSAIASSSAIASSSAIASSSAIASSSAIASSSAIASSSPVAPSSPVASSSPAVSSSAIVSSTPAVSTPVASSIPVISSPAIASGSAIASSSHVASSSTPAASSSPAVSSSPVASSSPALSSSPSASASSTPIIPSSTASSAVVSSSPTPSSSVVRSSSLLSSSSPALSSTRTPSNPVIPSSSAISITPSSTPVRSTSSVAPGKSSSAPVIPKPSSTVIATFTSSSGSLPSSSAPAGSGVPSSSTLPHPSSTSLLSSSPVSSAEPVSSSSAV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must have a Proton donor active site that is highly conserved among related enzymes.2. The protein can be expressed in plasma membrane and perform chitin binding without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "YIAGVVEYRPTFMGGTSEQLLQANLAGYLEIMASGNGTTDIIVFPEATLNSVITLTAVPKFTEQSLCEEQGDDDPEIAPFLRSLACAAREYGTYLVVNVKERVSEQCTSDETCSSRGYSIHNTNVVFDRQGAVISRYRKWNLYLEPSTNRTESPEIATFTTDFNVTFGHFICFDMLFYTPAQDLVEQLGIRHVIVTKMFNSELPFLTASQFQQGWAWANRVNLLASGGSLPQGGISGSGIYAGQQGALARLMITDELVGQRKLLLAKVPK",
    "response#2": "VAVTEHQFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNLLRWLGANAFRTSHYPYAEEVMQLCDRYGIVVIDESPGVGIVLVESYSNVSLQHHLEVMEELVRRDKNHPAVVMWSVANEPASFLKPAGYYFKTLIAHTKALDPSRPVTFVTNSNYEADLGAPYVDVICVNSYYSWYHDYGHMEVIQLQLATQFENWYRTYQKPIIQSEYGADTIAGFHQDPPLMFSEEYQKGLLEQYHLVLDQKRKEYVVGELIWNFADFMTNQSPQRVMGNKKGIFTRQRQPKGAAFLLRERYWKLY",
    "response#3": "KLLQQPELKNFTTWELPMVRGSSVDKRICQEDLCCEFRVTWTLEDTQPEYNYRLGVWVGQRRYEEEQYSAIRLCGLFACKGASVESCGLVSEEEVHLQDHRVVFTDLQILGEFVRRPRRLILPSTLSSSSFYALQPSQLAWSMEELANVTRIKMELRQPHSQLMTFAIYGNYFDC"
  },
  {
    "reference": "MSKYDSHLKCSFCGKSQEQVRKLIAGPGVYICDECVELCNEILDEELMDAPAAAVAGGGSPKPDDSRPKRRPSRERLSFNQIPKPREIKKYLDEYVIGQDEAKKVLSVAVYNHYKRLSLASKPGEETSEDHIELQKSNILLMGPTGSGKTLLAQTLAQILDVPFAVADATTLTEAGYVGEDVENILLRLLQVADLDVEEAQRGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKMLEGTIANVPPQGGRKHPYQDCIQIDTSNILFICGGAFVGLDKVIEQRRGKKSMGFIRPGDTNQSKEKRAADLMQQAEPDDLVKFGMIPEFVGRIPVMASLEPLDEETLIAILTQPRNALVKQYQKLLKMDNVQLEFNKDAVRAIAQEAYRRKTGARALRGIVEELMLDVMYELPSRKDVQRCLITKEMVEQRSTADLLVHPSSLPTPESA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must be optimized for zinc ion binding.2. The protein must exhibit the following characteristics: It directs the protease to specific substrates; Can perform chaperone functions in the absence of ClpP.; ATP-dependent specificity component of the Clp protease.3. The designed protein have ClpX-type ZB domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "QKSNILLIGPTGCGKTLLAQTLAKILDVPFAVADATTLTEAGYVGEDVENILLRLLQVADLDVEEAQRGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKMLEGTVANVPPQGGRKHPYQDCIQIDTSNILFICGGAFVGLEKVVDQRGGKKSIGFVQPGEGQSKEKRAADVLRHLEPDDLVKFGMIPEFIGRVPH",
    "response#2": "KSNILLIGPTGSGKTLLAETLARLLDVPFTIADATTLTEAGYVGEDVENIIQKILQKCDYDVEKAEQGIVYIDEIDKISRKADNPSITRDVSGEGVQQALLKLIEGTVASVPPQGGRKHPQQEFLQVNTANILFICGGAFAGLEKIIRSRSEQGGIGFAADVKSKDDSRNVGEVLADVEAEDLIRYGLIPEFVGRLPH",
    "response#3": "KSNILLIGPTGSGKTLLAETLARLLDVPFTIADATTLTEAGYVGEDVENIIQKILQKCDYDVEKAEQGIVYIDEIDKISRKADNPSITRDVSGEGVQQALLKLIEGTVASVPPQGGRKHPQQEFLQVNTANILFICGGAFAGLEKIIRSRSEQGGIGFAADVKSKDDSRNVGEVLADVEAEDLIRYGLIPEFVGRLPH"
  },
  {
    "reference": "MEVASWKEKKTLILGITGAIAAYKAADIVREFVKKEWKVHVMMTEGAESFVAPLTFSVLSGNKVFLEKDFLSDERGWEIPHITLSGAADVIAIAPCSASMLHKLAYGDASSLVSATVLASQSPVVVFPSMNSNMWDNKATQKNVEICKDLGYVVVEPEKGRLACGYEGKGRLPSIGVIVDEILKAACPVKNLLGKKVLVTAGPTYEYIDPVRYIGNPSSGKMGFAIAREAWYRGADVTVICGPVAEPYPHGVDVVPVVSAEEMCEATLARAEEADIIVKAAAVGDYRVKEKQLQKIKREGKETLSIELISNRDIAAELGKRKKKGQIIVGFAAETNEPIENARKKLKNKNLDMIVLNDITAPNAGFASDTNSVTLLTPDETLLEISTSKSIVAEKIWDVIYERFLCSK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The design of the protein must focus on the precise arrangement of amino acids in the Proton donor to enable effective catalysis of H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.2. The designed protein should be optimized for FMN binding, metal ion binding activity in order to improve coenzyme A biosynthetic process, pantothenate catabolic process.3. Mg(2+) is required for the proper folding and stability of the protein.4. We require a protein that can specifically bind to the metabolites produced in the CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis, Cofactor biosynthesis pathway.5. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.6. The presence of Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains is critical for the interaction of the designed protein with its intended targets.7. The designed protein should have a high specificity for CTP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "TIVVGVTGGVAAFKAPIVVRECQRAGHDVYVAATQASLDFVGRSTWEGITSRPVVVDIAGEGRAEHVELARIADLIIVVPATANSLAHLAAGFADDMVSLTVLASDAPVVVAPAMHSNMWLAPATQANVRTLRERGVHVIDPTSGALGSGDSGVGRLPEPELIARQALMELVRPAGTLAGRRLVVTAGGTREPIDPVRFLGNRSSGRQGLAIASAAAHAGGSVQVIAANIESALLSALPAGVQVTRVGSALQMRDATIAAVARADALVMTAAVADFRPEVASVSKMKKDPTNEDVPTIRLIRNPDILAEIGHSEQRPPLVVGFAAETGTDEEILAYGADKAARKGADFICLNRVGESVGFGDVPNDIRVLGADGSERDVALGSKRTIADAVWDLVVSRLPEAS",
    "response#2": "VALGVTGGIGAYKAVEIARGLQKRGHDVVAIMSHGALKFVGEVTFEAITKRRVITDQYAPGANADIEHISLATDIGLLLVAPATANVIGKFAHGIADDFLTSLYLATRAPVLMAPAMNTNMLEHDAVSRNLATLTARGVRFVDPGQGYLACGWVGKGRLAEPDDVVEALEAVAGPSADDDGLRVLVSAGGTREAIDAVRYIGNRSSGRMGFAIADEAAARGAHVEVVAANVELPRSPGIGYQAVESAAELAAACRARFAECDVLVMCAAVVDFRPTLEAEGKLKKGAAQSLELRLERTEDVLRGLADRRAPGQTLVAFAAEHGDGSLARGRAKLRDKGVDLLVVNDIAKQGIGFDAQDNEVWLIDATGAQEFSRRSKREVAAAVLDRVVGLLAPPG",
    "response#3": "ILLGVTGCIAAYKSCEILRLLQKAGHDVKVVMTEHATEFVGPTTFRALSGHEVAVGLFDEPGDPIHHVSLAKEPDVFLVAPATANVIAKIARGVADDLLTTTALATKAPLVIAPAMNVEMWRDVVTQENIATLEARGCTIVYPDSGYLACGDVGAGRLAEPEAIVQATLDEAAAAATLLARPAADGLRVLVSAGGTREAIDAVRYIGNRSSGRMGFAIADEAAARGAHVEVVAANVELPRSPGIGYQAVESAAELAAACRARFAECDVLVMCAAVVDFRPTLEAEGKLKKGAAQSLELRLERTEDVLRGLADRRAPGQTLVAFAAEHGDGSLARGRAKLRDKGVDLLVVNDIAKQGIGFDAQDNEVWLVGVDGDVVALAGRHVVVSAGGTREPLDPVRFLGNRSSGRQGYALAAVAAARGARVTLVAANVELPDPAGTEVVAVTGARDLCDAVLAAAAVADAVVMAAAVADFRPSSAQDHKIKKDGSAPAPIALSENPDVLAELVARRRPDQVLVGFAAETGDAAGSVLEHGRAKLARKGCDLLVVNAVGDGQAFGRPDNAAVDVLWADGDATDLPLESKRGVADALWSRVVALRER"
  },
  {
    "reference": "MTADLYSADHARALDSADPLAGFRAEFLLPRHDGHEQAYFCGNSLGLQPKGARAHVEEVLDKWAAEAVEGHFTGQAQWMPYHELVRDPLADVVGAQPSEVVAMNSLTANLHLMMVSFYQPTIERTAILVEAGSFPSDRYAVESQLKYRGLPPGHTLIEVEPDNADGTFSMEAIERAIRADGKRLALVLWPGVQYRTGQAFDLKEIARIGHEVGAIVGFDLAHAVGNLPLQLHDSGADFAVWCHYKYMNAGPGAVAGCFVHERHARTHRPRFAGWWGHEQASRFRMGPEFVPTPGADGWQLSNPPILALAPLRASLDLFARAGMDALRAKSESLTGYLEALIDLRLRDTLQIITPRDPSQRGCQLSLRVTGGCGLSGRDAGRALFETLGGQGIVGDWREPDVIRISPAPLYNTHADVLRFARAVEQWRSAGA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein with 3-hydroxykynureninase activity, pyridoxal phosphate binding is needed to enhance L-kynurenine catabolic process in a specific context.2. Ideal protein should have a binding affinity for key enzymes in the NAD(+) biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "HREWMDIANTVTPMLADLIGAKEREVACMGSLTANLHLMLSAFYKPTPSRYKILCESKAFPSDQYAFASQALFHGYDPKDAIVEVFPREGEYNLRVSDILDEIAKQGSAIALVIFSGVQYYTGQLFPMKVITRAAKEHGCLCGWDLAHAIGNVPMSLHEWDVDFAVWCSYKYLNSGPGGIGGLFVHEKWESTKTPQFAGWWGHDPSTRFEMPPMFSPIPGAQGFQQSNPSVFATVSLLGSLQVFKQVGMMQPLRERSVSLTARLEALLRD",
    "response#2": "HREWMDIANTVTPMLADLIGAKEREVACMGSLTANLHLMLSAFYKPTPSRYKILCESKAFPSDQYAFASQALFHGYDPKDAIVEVFPREGEYNLRVSDILDEIAKQGSAIALVIFSGVQYYTGQLFPMKVITRAAKEHGCLCGWDLAHAIGNVPMSLHEWDVDFAVWCSYKYLNSGPGGIGGLFVHEKWESTKTPQFAGWWGHDPSTRFEMPPMFSPIPGAQGFQQSNPSVFATVSLLGSLQVFKQVGMMQPLRERSVSLTARLEALLRAR",
    "response#3": "HREWMDIANTVTPMLADLIGAKEREVACMGSLTANLHLMLSAFYKPTPSRYKILCESKAFPSDQYAFASQALFHGYDPKDAIVEVFPREGEYNLRVSDILDEIAKQGSAIALVIFSGVQYYTGQLFPMKVITRAAKEHGCLCGWDLAHAIGNVPMSLHEWDVDFAVWCSYKYLNSGPGGIGGLFVHEKWESTKTPQFAGWWGHDPSTRFEMPPMFSPIPGAQGFQQSNPSVFATVSLLGSLQVFKQVGMMQPLRERSVSLTARLEALLRE"
  },
  {
    "reference": "MAKTTAAITFVTGNAKKLQEVQQILGQGFPFELTNRKIDLPELQGEPEDISREKCRLAAAEVKGPVMVEDTSLCFNALHGLPGPYIKWFLDKTGHVGLNNLLAAYPDKSAYAQCIFAFTTGPGAEIQTFVGRTEGKIVPARGPTDFGWDPVFQPDGFEETYAEMDKTIKNSISHRGRSLSALCAYFDTHKAELEKQLAA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.2. Mg(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "PTLILLHGFPSSSHQYRHLLADTHLTSKYHLIAPDYIGYGQSSMPSTKDFEYTFDNLSVVTEKFIKALSIEKYSLYVFDYGAPIGYRLALRNPERIQSFIVQNGNAYDEGIDNAFWSNVKKYWAEPTKQENIDFVLGLITPQTTQWQYLNGVQEKDKSLVAPDGYVSDQYYLDRPGNGEIQAQLLFDYGTNPAKYPAFQQYFRQYQPPMLIVWGKNDEIFPAEGAYPYKRDLKNLEFHLFETGHFTLETPPELAAKL",
    "response#2": "PTLILLHGFPSSSHQYRHLLADTHLTSKYHLIAPDYIGYGQSSMPSTKDFEYTFDNLSVVTEKFIKALSIEKYSLYVFDYGAPIGYRLALRNPERIQSFIVQNGNAYDEGIDNAFWSNVKKYWAEPTKQENIDFVLGLITPQTTQWQYLNGVQEKDKSLVAPDGYVSDQYYLDRPGNGEIQAQLLFDYGTNPAKYPAFQQYFRQYQPPMLIVWGKNDEIFPAEGAYPYKRDLKNLEFHLFETGHFTLETPAEIAAR",
    "response#3": "PTLILLHGFPSSSHQYRHLLADTHLTSKYHLIAPDYIGYGQSSMPSTKDFEYTFDNLSVVTEKFIKALSIEKYSLYVFDYGAPIGYRLALRNPERIQSFIVQNGNAYDEGIDNAFWSNVKKYWAEPTKQENIDFVLGLITPQTTQWQYLNGVQEKDKSLVAPDGYVSDQYYLDRPGNGEIQAQLLFDYGTNPAKYPAFQQYFRQYQPPMLIVWGKNDEIFPAEGAYPYKRDLKNLEFHLFETGHFTLETPEEMAPFLTRLR"
  },
  {
    "reference": "MPVIRFYDGSQQVYEHSVSLIEIIKNKKPSIMKSLIAISVNNHFSNLNTFIREDAFIEFVDQKNYKALNIIRYSCAQLLSYAIKNIWPLAQIATSNIIEDGFYCDIDFKRSISEKDLILLENQMKTLVKREYNILNKLISYSEAREIFQKCFEKYKVSLIDENINCNSKVSLYYHENYADIDIGLQVFNIKFCKYFKLQKIGGVYWKKNKNNKMLQRIYGTAWTNKQELDKHLDYLNELEKRDHRKIGKFLQLYHMQEESPGMIFWHNKGWIIFNELQNFVRVKLKEYKYEEVKTPLLIDKLIWKQSGHWDNYKNAIFTTLSEHREYCIKPMNCPGHVQIFNSRLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRNFTQDDAHIFCTREQVRSEINDCIKMIYDLYSTFHFKKILVKLSTRPEKRIGTDSLWNESEKDLSDMLIENHLSFEYQSGEGAFYGPKIEFILQDSLDRNWQCGTIQLDFYLPLRLSSFYINEKNEKKVPVIIHRAILGSIERFIGILIEECSGNLPTWLSPVQVVIISITDISSGYVKELFKKFSDVNIRIECDLRNEKIGFKIREHTLRRIPYILICGEKESSSKKISVRNRQGHNFGMIDVDFFIKKLQKEIITRNFYQMEE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that can perform tRNA binding, threonine-tRNA ligase activity in cytoplasm to support threonyl-tRNA aminoacylation.2. The protein must exhibit the following characteristics: Also edits incorrectly charged L-seryl-tRNA(Thr)..3. The designed protein should contain one or more TGS domains that are essential for its function. The designed protein sequence is ",
    "response#1": "WHPKGWTIWQGVEQYMRKVYQDNGYQEVKGPQVIDKTLWEKTGHWDKYRDNMFTTESEKREYALKPMNCPGHILIFKQGIKSYRDLPLRYGEFGQCHRNEPSGGLHGIMRVRGFTQDDGHIFCTEEQILKECVDYTTLLQKVYTDFGFKNIIYKVATRPDARIGSDESWDKAEQALIESLRASGCEFEISPGEGAFYGPKIEYTLKDAIGRQWQCGTMQVDFSMPERLDAEYVGEDGARHRPVMLHRAIVGSLERFIGILIEEHAGALR",
    "response#2": "DHRKIAKDMDLFHIQEEAVGQVFWHEQGYILYNVLESYIRKKLINNGYFEVKTPILVSKELWEKSGHWDKFRENMFIVDESESKKLAIKPMNCPCHVQIFNSHTRSYRDLPIRMAEFGTCHRNESSGSLHGLMRVRGFTQDDAHIFCMEEQVNSETIKFCDLLKEVYSELGFNEISVKFSDRPDVRAGDDEVWDRAEKALLEAVKEAGLSYELSPGEGAFYGPKLEFVLKDAIGRSWQCGTLQVDFILPERLGAFYIGADGHKHHPVMLHRAILGTFERFIGILIENYAGKFR",
    "response#3": "RDHRKIGRQMDLFHMQEEAVGSVFWHEKGWRLYRTVEAYMRRRQEEFGYIEVRTPQLLDRKLWEASGHWDNYRSHMFIAEVEDEDKVLAVKPMNCPCHVQIFNHGLRSYRELPLRMAEFGACHRYEPSGALHGIMRVRAFAQDDAHIFCTEDQIADETVRFVEMLRRVYADFGFPEFAVKFADRPTPRSGSDEVWDKAEAALRSACETAGVEFTVNPGEGAFYGPKLEFVLRDAIGRDWQCGTLQVDFVLPERLDASYVTAESGRARPVMLHRAIVGSFERFIGILIENCAGRFPLWLAP"
  },
  {
    "reference": "MINVTDNIEAKKVDTLLLNEYNYTEDVLIQNAAHSLFNEIDKTVNKYIVAVGPGNNGADGLALAILLHSINKDVKIICKKNMSIYHKIALKIGIPSIEHVQSCDILIDALFGFGFSGPVQGYYKELIDEINEKKDYKIISIDLNTAHINSDCVLMLSTYKQTLLNSSIPKKVLKIGVNEKIYECVSNKYIVDEEYIGSIKKEKNEFCNKSDFGRVKIYAKKGAALLASRASVKSGSGYTYLISDDETRKANLSFNPECINSDTTKYDVICLGPNNGVDFDYASIIYTNLDKKMVIDADALTYISNNLEILDSLTEDCVLTPHPLEFSRISGYDLNEILEKPFEKLEEFGKKYKGTIILKGKNNIIYDGHKFYIVNIGNSKMANAGMGDLLSGMIASYLAQGYKTRDAVIYATYQQAKIGRYLSEKKDTINPTDILSLINNK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.2. A protein with ATP binding, ADP-dependent NAD(P)H-hydrate dehydratase activity, metal ion binding, NADHX epimerase activity, NADPHX epimerase activity is needed to enhance nicotinamide nucleotide metabolic process in a specific context.3. For general function, the protein need meet that Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP; Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.4. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.5. The designed protein should be able to compete with endogenous proteins or inhibitors for (6S)-NADPHX, AMP binding. The designed protein sequence is ",
    "response#1": "MQRLEMAAVTAGITLAQLMENAGAALAQEVESRCRPIQGKRAVLLCGKGNNGGDGFVCAGLLAQRGMTCTVVLTQGEPKTDLAGDAFSRMPDQVQVISGAEEREQAEAAMTAADVIVDCVFGFSFRGELSGVPAHFLGYAGNLSCLKISADLPSGVECDSGRVSAGAFRADVTVTFTGKKPANVSYPAKEFCGETVVRQVGVPAALVGREALDGWLSPRRRNAHKGDSGHVLCVGGDTGHGGAVMLAADAALRCGAGLASVATRSAHVPGLLARRPEAMARGVDGAAALAPLLARADVVALGPGLGQGSWGRALYGAVLAAGLPLVLDADALNLLAAAPRELAADTILTPHPGEAARLLGMETTQVQADRFAAAARLVERYGCVVVLKGAGTVVAAPGQSPRVVAAGNPGMAVGGMGDLLTGCIAALRARGMPAFEAASLGALLHALAGDAAARDGETGLLPSDLLPWGREALDGWLSPRRRNAHKGDSGHVLCVGGDTGHGGAVMLAADAALRCGAGLASVATRSAHVPGLLARRPEAMARGVDGAAALAPLLARADVVALGPGLGQGSWGRALYGAVLAAGLPLVLDADALNLLAAAPRELAADTILTPHPGEAARLLGMETTQVQADRFAAAARLVERYGCVVVLKGAGTVVAAPGQSPRVVAAGNPGMAVGGMGDLLTGCIAALRARGMPAFEAASLGALLHALAGDAAARDGETGLLPSDLLPWLRALAE",
    "response#2": "MAMADAAAIAGGVPGADLMEAAGRAVAEAVLERHLPRPVVVLCGPGNNGGDGFVAARHLHAAGWPVRVALLGDRSALHGDAASASQAWKGPVEALSLGLLDGHPLVIDALFGAGLGRPIDGIAGELIDRMNAEALHVVSVDIPSGLHGDSGEVMGCAPMAECTVTFFHAKPGHYSVEGLRRCGSLRVVDIGIARSVLEGLEGRVLCVGGDTGHGGAVMLAADAALRCGAGLASVATRSAHVPGLLARRPEAMARGVDGAAALAPLLARADVVALGPGLGQGSWGRALYGAVLAAGLPLVLDADALNLLAAAPRELAADTILTPHPGEAARLLGMETTQVQADRFAAAARLVERYGCVVVLKGAGTVVAAPGQSPRVVAAGNPGMAVGGMGDLLTGCIAALRARGMPAFEAASLGALLHALAGDAAARDGETGLLPSDLFLTAESIALLLRRRDRQAHKGHFGHCLIVAGSSGKTGAAALSANSAVRAGSGLVTLAVPESLNPILEVKTTEAMTVPLADSGGGYLTDSAFPAIAGLLPDKDALAIGPGLGQHPATVTLVRALLKAADLPLVIDADGLNAVAQNANCLLDKKSTHVVLTPHPGEMARLMGATIADVEADRIATARKFAAAFGVHLILKGSRTIIAAPDGSTAINGSGNPGMASGGMGDVLTGIIVSLLGQGYNCHDACRLGVFIHGYAADLVAQDKGEIGMCAGDVQEKLPYL",
    "response#3": "MLRPLYTADEMRAAEQGHDVEAMMERAGSAVAETVLRRYPDARRIAAVCGKGANGGDGRIALRVLAEAGRETGEELDGADVVIDALFGTGFHGAPRDDAAGRIEQINGAGVPIVAVDLPSGVDASTGEVVGAVVDADVTVTMHGPKVGLEVGPGRFHAGEVEVAEIGLGAGSGDVLTGIELDGADVVIDALFGTGFHGAPRDDAAGRIEQINGAGVPIVAVDLPSGVDASTGEVVGAVVDADVTVTMHGPKVALGPGDVSIVVKPRRPEAHKGDFGRLLIVGGSETFSGAPALAALAALRTGVDLVYIAAPRETAYSISSMSPDLITLKLEGDHLSMRNMPIIRNYMEKVTAVAVGPGLGLHKETQEAVSEIIRIAEERKMPILLDADGLKAFAGFKRRLETPAVLTPHLGEYAILTSEKPPDNLEERGNHVKKTAENLNATILLKSHVDIISDGFKTKFNFTGNPGMTVGGTGDVLTGIVAAFLAQGVDPFEAAVAGAFINGAAGDFVKAEKGYHMVATDLLDWIPKVIDDFLTAESIALLLRRRDRQAHKGHFGHCLIVAGSSGKTGAAALSANSAVRAGSGLVTLAVPESLNPILEVKTTEAMTVPLADSGGGYLTDSAFPAIAGLLPDKDALAIGPGLGQHPATVTLVRALLKAADLPLVIDADGLNAVAQNANCLLDKKSTHVVLTPHPGEMARLMGATIADVEADRIATARKFAAAFGVHLILKGSRTIIAAPDGSTAINGSGNPGMASGGMGDVLTGIIVSLLGQGYNCHDACRLGVFIHGYAADLVAQDKGEIGMCAGDVQEKLPY"
  },
  {
    "reference": "MINLFDLDKAGLEAFFIEHGEKRFRAAQVLKWIYHEGELTIQRWTNLSKDCRAFLQEKTQLLLPKIITSQHSADGTVKWMLQLPDTSGAKNAVEMVFIPENDRGTLCISSQAGCALACPFCSTAHEGFNRNLSSAEIIGQVWLALNELGWQKGGKRKVTNVVLMGMGEPLLNYKNVVRACNLMVDDLGFGLSRRRVTLSTSGVVPKLYDLKKDSDVALAVSLHASTDALRDRIVPINQKYPLAELMAACRDFVDNREAKKHITWEYVMLKGVNDSIEDAKALHRLISGIPGKVNLIPFNIFPGTQFEPSDSRQIVKFQNFLAQKGVVITVRRTRGEDISAACGQLVGEVKDRSRRERILVRAE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The presence of [4Fe-4S] cluster is crucial for the protein to function optimally under specific conditions.2. The designed protein should contain one or more Radical SAM core domains that are essential for its function.3. The [4Fe-4S] cluster binding site should be located in a region of the protein that is accessible to the ligand.4. The protein must exhibit tRNA (adenine-C2-)-methyltransferase activity, rRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding. The designed protein sequence is ",
    "response#1": "EDGRGTLCVSSQVGCALECSFCSTARQGFNRNLSAAEIIGQLWVAGRELGEGRVTNVVLMGMGEPLLNFNNVVAAVSLMLDDFAYGLSKRRVTISTSGIVPALDRLAEVSDIALAVSLHAPDDELRNELVPINRKYPIKELLAACKRYVGRENRRKVTFEYVMLDGINDTPAHARALIRLLSHVPSKVNLIPFNPFPGSNYRCSGPETIRRFSDMLHAAGLIATTRKTRGDRAED",
    "response#2": "MTACVSSQVGCSLTCKFCATGYMDRKRNLDPAEIYDQVVLIDQQARENYGIPLSNIVYMGMGEPLLNYANVMKSIERITSEDGLNMSAKRITVSTAGIAKMIKKLGDDQVKFNLALSLHAANDAKRNEIMPINEQNSLKALAEALKYYYAKTKNPVTYEYIIFDNFNDEIQDAMELARFCKHLPCKVNIIEYNPISFAAFENTTEDKTEAFANYLRG",
    "response#3": "MTACVSSQVGCSLTCKFCATGYMDRKRNLDPAEIYDQVVLIDQQARENYGIPLSNIVYMGMGEPLLNYANVMKSIERITSEDGLNMSAKRITVSTAGIAKMIKKLGDDQVKFNLALSLHAANDAKRNEIMPINEQNSLKALAEALKYYYAKTKNPVTYEYIIFDNFNDEIQDAMELARFCKHLPCKVNIIEYNPISFAAFENTTEDKTEAFANYLRES"
  },
  {
    "reference": "MFQSLIAIDYGKARIGISSGQMITKTATPIGTVEAYDGVPNWIELDKIIKCWNPSDIIIGLPLDTQNFETDITKSAKDFAKEVQQRYQRKVHLINEAYSTREARWRLEEVKSKKVSHIKVDALAACVILETWMSEN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be able to regulate rRNA 5'-end processing. The designed protein sequence is ",
    "response#1": "HVVSMLRLAKSVADLYPSVNRDLLYAGVILHDLGKVIELSGPVSTTYTLEGNLIGHISIVVEEVSKIADELSIDGEEVVVLKHVLLSHHGKGEWGSPKPPLVREAEILHQIDLMDASLNMV",
    "response#2": "MRKLGIDFGLSRIGFAISDESNSFSLALENFDYDGSYEKIIQKIQLFLDEYKIDKFIIGYPLNSRGEKTKTCLLIDEFIIHLEKNFDQKIKLINESYSSRKADKRY",
    "response#3": "HVVSMLRLAKSVADLYPSVNRDLLYAGVILHDLGKVIELSGPVSTTYTLEGNLIGHISIVVEEVSKIADELSIDGEEVVVLKHVLLSHHGKGEWGSPKPPLVREAEILHQIDLMDASLNMI"
  },
  {
    "reference": "MQTLSGKRILLGITGGIAAYKCAELARTLIELGADIRVVMTQTATKFITPLTMQTISGYPISFSCSGKKYSMKHIELAKWPDLILLVPATANLIANMRVGIANDLLSTLILATNTPISIAPAMNQNMYLNRATQENLTILAERGINIWGPKSGKQACGNLGLGRMLEPIQLVERCEVFFQIKCLQGKSILISAGPTHEALDPVRYITNYSSGKMGYALAEAAKLFGAKVTLVSGPVSISKPKNITRISVKSALEMHQTILNHAIKHDVFISCAAVADYRPKTISSQKIKKTDNRTQMNIRLVKNPDIVADVGKLVKNRPFTVGFSAETQNIEKNTLDKLIKKNLDIICANNVACSNQGFNSDYNAITIYEKQKKTQLPLTSKTKLAKAILLYISECL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The presence of Mg(2+) is necessary for the protein to function in the desired environment.2. The Proton donor of the protein must be engineered to enable effective catalysis of H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.3. The CTP binding site should be located in a region of the protein that is accessible to the ligand.4. The protein design must be able to perform phosphopantothenate--cysteine ligase activity in order to contribute to coenzyme A biosynthetic process, pantothenate catabolic process.5. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A The designed protein sequence is ",
    "response#1": "SIVVGVSGGIAAYKACAAVRLFKEAGHRVTVVATRAALDLVGAVTWEALSGRRVYTDVCEDAADVAHVRLAREADAIVVVPATANTLAKIRAGIADNMLTSVVSAATCPVFVAPAMHTEMWFNPATQENVAVLRERGVRFIGPASGRLTGADSGVGRLSEPGDVVAAVLSALSGARPSRGTRLTGKKAIVTSGPTHEPIDPVRYIANRSSGKQGHAIAASLAKLGCDVTLVSGPTQEPAPNGVDLIKIESARDMLQACENALPADIAVCAAAVADWRVDIEADQKLKKDGSGQMPDLKLAENPDILATLSQHKDKRPSLVVGFAAETEKVIEHATAKRERKNCDWIVANDVSPSTGTFGGNENTVHLVTAETVTEWPAMTKTQVAERLAARIADALL",
    "response#2": "SIVVGVSGGIAAYKACAAVRLFKEAGHRVTVVATRAALDLVGAVTWEALSGRRVYTDVCEDAADVAHVRLAREADAIVVVPATANTLAKIRAGIADNMLTSVVSAATCPVFVAPAMHTEMWFNPATQENVAVLRERGVRFIGPASGRLTGADSGVGRLSEPGDVVAAVLSVLARGARSGPGLTFAVSAGGTREPIDPVRYLGNRSTGRFGTAITRLAAERGAKAILVESNVASDVLREAGDAEIRHAPSALELRDAMQAASLEADVLVMAAAVADFRPAKVSPTKQKKTGESFLPIELVENPDVLAGLAAARRPGQTIVGFAAETGDERCSVEDYGREKALRKGADLTVVNGVGEGIGFGDVDSRIVDVTGAEASVPHGPKEVLADLLWERVPQLIGE",
    "response#3": "KHVLLVVSGSVSAYKALELTRLLRKAGARVTPVLTAGGARFVTPNALQAIAGEPVHQDLWSLQGEADVGHIRLARMADLVAVVPASADFLAKMAHGLADDLASTVLLATDRPVLVAPAMNWAMWAHPATRANMAALTARGVQAIGPNDGAMAEAESGPGRLAEPSEIFAAISRALGA"
  },
  {
    "reference": "MSADRNLLPLLAGITTVSRERDSRVGGMTLDSRTVRPGDLFIALPGLRDDGRAHIPDAVARGAAAVFYEPEGYAPPALAVPAIAVPRLREQLGVIASRFYDAPSRRLKVIGVTGTNGKTTCTQLLAQALDRPPDRCGVIGTLGNGFPGALDEGTHTTPDAAAVHGLLAEFLANGARAVAMEVSSHALEQGRVNGVCFEAALFTNLSRDHLDYHGDMATYGAAKSRLFMQDGLKAAVVNVEDEYGERLARALRDRVRLVGYGLRRGDVHARALACTRGGIELEALTPMGEVGIRSPLFGEFNALNLLGALATLLALGLDPREAGRRLAAALPVPGRAERFAGGPNRPLVVVDYAHTPDALEQVLKSLRPHAAGRLWCVFGCGGDRDRGKRPEMGAVAERLADVVLITDDNPRHESGDAIVADILEGMRSKPRVIRDRRAAIATALADAAAQDVVLIAGKGHEDYQQVGDVRHPYSDRDTVRALLGLAA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.2. The protein design must feature the Meso-diaminopimelate recognition motif motif.3. The protein should have magnesium ion binding and be active in cytoplasm. The designed protein sequence is ",
    "response#1": "PHASNKRATILDDAGAWFGFEQEYFFYKDGRPLGFPTSGYPAPQGPYYTGVGFSNVGDVARKIVEEHLDLCLAAGINHEGINAEVAKGQWEFQIFGKGSKKAADEMWMARYLMLRLTEKYGIDIEFHCKPLGDTDWNGSGMHANFSTEYMRTVGGKEYFEALMAAFDKNLMDHIAVYGPDNDKRLTGKHETAPWNKFSYGVADRGASIRVPHSFVNNGYKGYLEDRRPNSQGDPYQIASQILKTV",
    "response#2": "AWFGFEQEYFFYKDGRPLGFPTSGYPAPQGPYYTGVGFSNVGDVARKIVEEHLDLCLAAGINHEGINAEVAKGQWEFQIFGKGSKKAADEMWMARYLMLRLTEKYGIDIEFHCKPLGDTDWNGSGMHANFSTEYMRTVGGKEYFEALMAAFDKNLMDHIAVYGPDNDKRLTGKHETAPWNKFSYGVADRGASIRVPHSFVNNGYKGYLEDRRPNSQGDPYQIASQIL",
    "response#3": "PHASNKRATILDDAGAWFGFEQEYFFYKDGRPLGFPTSGYPAPQGPYYTGVGFSNVGDVARKIVEEHLDLCLAAGINHEGINAEVAKGQWEFQIFGKGSKKAADEMWMARYLMLRLTEKYGIDIEFHCKPLGDTDWNGSGMHANFSTEYMRTVGGKEYFEALMAAFDKNLMDHIAVYGPDNDKRLTGKHETAPWNKFSYGVADRGASIRVPHSFVNNGYKGYLEDRRPNSQGDPYQIASQILKTL"
  },
  {
    "reference": "MSSAPVAPSTYTPLNLLKEGTVVNVCGVVKFFKPPYLSKGTDYCSVVTIVDQTNVKLTCMLFSRDYDTLPSVYKVGDIVCFQGLKIQAYKNELQAISKFGFASLTFDGTLGAPIILRTLSKRFNFTAQDYSMVEALRTWASTHISASSNLVQLSDAQPMQYYDLTCQLLGKAQVDGVSFLLKVWDGTRTKIPSWRVCIQDVAFEGDLSHILQLQNLVVDIVVYDNHVQVAKSLKIGSFLRIYSLHTKLQPINSESTTSLVRLEFHLHGGTSYGRGIRVLPESNYDVDQLKKALESVDLEAIQTSDDTYQSDNVENCLTSSSSESDLVSLYEVERCQPLCATSKPFSSVVTDT",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be involved in establishment of protein localization to telomere, regulation of double-strand break repair via nonhomologous end joining, telomere maintenance in response to DNA damage by localizing to chromosome, telomeric region and exhibiting G-rich strand telomeric DNA binding, small GTPase binding, single-stranded telomeric DNA binding.2. The designed protein must have at least one Telomeric single stranded DNA binding POT1/Cdc13 domain. The designed protein sequence is ",
    "response#1": "HPWLENGLLNLIKNADVLPIRVFKCQPLQIFEYIRYEHPIRCKLSDTEFYIEAEFSSQSISDLNNFTEKRITSLRGGIVTLGNFLIHLIPSQSGIIPWIQVESFNFQGCEGAVFGNPKAIA",
    "response#2": "QLSEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGFQPPRFCFQAKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVL",
    "response#3": "HPWLENGLLNLIKNADVLPIRVFKCQPLQIFEYIRYEHPIRCKLSDTEFYIEAEFSSQSISDLNNFTEKRITSLRGGIVTLGNFLIHLIPSQSGIIPWIQVESFNFQGCEGAVFGNPKAIA"
  },
  {
    "reference": "MNIIFASQNANKIAEIRAKLPEHTIEGLDAKAFPNELLETGDTLDANALQKVRQVFEKTGKNCFADDTGLEVEALNGAPGVYSARYAGEQKISEDNMNLLLHNLKGETNSKARFRTVVALIWEGKEYFFEGVCEGEITTAKSGEKGFGYDPIFRPNGYDRTFAEMTMDEKSTISHRGLAIDKLVEFLAGK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be designed to have high stability and activity towards dITP + H2O = dIMP + diphosphate + H(+).2. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.3. The designed protein must be able to perform ribonucleoside triphosphate phosphatase activity under specific conditions.4. The substrate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MKFVLATKNEGKVRELQALLGGMELLSLRDLPDFPEAPETGATFLENARLKAHALAEKTGLPVLADDSGLEVDALNGAPGVYSARFAGEDASDAANRDKLLSDLEGVPEAKRGARFRCVAAFASPGGEILSVEGVCEGWIAEAPRGTGGFGYDPVFYLPEYGCSAAELTPEQKNAISHRGQALRRLLAALG",
    "response#2": "MPDTIYAVIATKNAGKTRFLENFAPPLPEGYRVIALDQGE",
    "response#3": "MTAIQGRLLVATHNPGKVREIGALLAPYGVEVTSAGALGLPEPVETETTFVANAELKARAAATVSGLPALADDSGLCVDALDGAPGIYSARWAGPDKDFAAAMARIEEELTAKGSTGGAHFACAAALAWPDGHTETFEGRVDGSLVWPPRGDHGHGYDPIFLPTGDTRTFAEMTAEEKHGVSHRADAFAKLVAACFG"
  },
  {
    "reference": "MVMSSPTNSARIILISGIASGQGKTTVTAALARKLIRQGLRVRVFKTGPDYLDPMILQRASGAEVYALDLWMVGLDDCRRLLARAASEVDVILIEGVMGLYDGDPSSADLARAFGVPVVVVLDAAKMAQTVGAVVLGLQQYGPVDLAGVIVNRLASPSHASMVTRGIRNVPILATLPKQQQALPERHLGLVQPDEIAQVDQVLDQLADQIEIDMVAWDAIAPVVLDGSLAAASTQQLLAGKTIAIARDAAFAFVYHANLECLRAAGAQLKFFSPLNDETIPAEADAVYIPGGYPELHCATLSSAQRWQDSMRAAHVRNMPILAECGGMMVIADSLIDAQGKKWPMVGLIPGEVAMQGKLAGLGMQSLATEDGELRGHAFHYSTLSTPVEPQAQTVKRSNGAHGEAVYKQGALTATYFHAYFSSCPAATARIFSPEIKA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design must be able to perform ATP binding in order to contribute to glutamine metabolic process.2. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile to enable effective catalysis of 2 ATP + cob(II)yrinate + 2 H2O + 2 L-glutamine = 2 ADP +   cob(II)yrinate a,c diamide + 2 H(+) + 2 L-glutamate + 2 phosphate.3. A protein that is capable of catalyzing reactions within the cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 10/10, Cofactor biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "VAMASGAAFTFSYAEHTELLAAAGAEVVTFDPLRDEELPEGTQGLVIGGGFPEVYASELSANEGLRKSVAELAFSGAPVAAECAGLLYLCRELDGLPMCGVLDAAARMSKRLTLGYRDAVAVSDSALAVAGTRMRGHEFHRTVVEPGAGAAPAWGMRAPERRVEGFVERGVHASYLHTHWAAR",
    "response#2": "IMIAGTGSGSGKTTIVCGLCQCIKDIGRTPSALKCGPDYIDAMFHSRVLKMRTGNLDSWFCDKTTIRTLLAKKEKSSDITVIEGVMGYYDGAGFSTKGSSFEIAQITDTPVILIVNCRGISNSVGAVLKGFTTFQENSQIRGVIFNQMSEKLYPQAKKAAQELGLIPLGFLPYKP",
    "response#3": "SATRKSSGKTFIAVGLTAALSARGLVVQPFKKGPDYIDPRWHSLAAGRECRNLDDFIMGRPKVLTSFVAHAQGADVAIIEGNLGLFDGQDLEGSDSSAALAKALGAPVLLVVDCKHLARSVAPLVCGHLHFPGGETIVGIILNNVATPRQEKRLREAIERFCPIPILGAIPRSAEIMS"
  },
  {
    "reference": "MGESDGNGAPTRRRRATLSAGRGATLTPMNGRSAAAFQGTDAADISSKDGGQQGKRDRSLETVRSTSKSKKKRRNDISDTLSCHKTQQSLLSSASGYKNYRGVLNWCVVMLVLSNARLFLENLLRHGILVDPIQVISLFLNDPYSWPAACLLIVSNVFVLVALYTERQLSKGSLSELAGFLVHCINMAIMLTFPAAVVLLVPSVTPVGGVFVLGTYTILLLKLYSYKDVNMWCRELSNVKAKKLARSLSCPSAQHLNGGDGQVCYPGNLSLRDMYYFVFAPTLCYELNFPRSPTIRMSFLLRRLFEMLFFTQLLVALTQQWMIPIIQSSMKPLEDMDLSRMTERLLRLAVPNHLLWLMFFYWFFHSSLNFTAELLRFGDRQFYKDWWNSESVTYFWQNWNIPVHKWCLRHFYKPLLRRGFSKMISQSAVFFLSAFFHEYLVSVPLRMFRLWAFMGMMAQLPLAWFVGHFLRGNYGNAAVWMSLIIGQPFAVLMYVHDYYVLHYRQETD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. We are looking for a protein with broad substrate specificity for hexadecane-1,2-diol + hexadecanoyl-CoA = 2-hydroxyhexadecyl   hexadecanoate + CoA.2. The protein should be optimized for activity in Lipid metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "FDVQAACSGFIYAVTIADSLIKSNDRIKYALVIGAEIMSRIVDWKDRSTCVLFGDGAGAVIMKSTAHCNEMTENSTRGIISTNLYSDE",
    "response#2": "FDVQAACSGFIYAVTIADSLIKSNDRIKYALVIGAEIMSRIVDWKDRSTCVLFGDGAGAVIMKSTAHCNEMTENSTRGIISTNLYSDR",
    "response#3": "FDVQAACSGFIYAVTIADSLIKSNDRIKYALVIGAEIMSRIVDWKDRSTCVLFGDGAGAVIMKSTAHCNEMTENSTRGIISTNLYSDR"
  },
  {
    "reference": "MKQVIIDLQLVCENTDNLPSEAQIQAWANRAIQPEFSDVEMTVRIVDEAESHDLNLTYRGKDKPTNVLSFPFECPDEVELSLLGDLVICRQVVEKEAEEQGKPLMAHWAHMVVHGCLHLLGYDHIEDAEAEEMEGLETEIMQSLGFDDPYLSEKEMHG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining endoribonuclease activity. The designed protein sequence is ",
    "response#1": "GLLLRAGQRDSLGYFATRHDKSVIFSPRGDGAVTYRLLAAVSLASADPIGPRSAWAGAIEAWLAETRRFGWFPAVLAASEEGARAYVTAGLKALPVGDEAIIEVAEFSLEGATMEPVRRAVRRVRRAGYTFTVARHGTLSAGELAEIERRAEDWRGDDTERGFSMALGRLGDPADEACVAVLARDEAGRLRGVLSLVPWGSHGLSLDLMRRDRGAENGLVEAMVAALVQAARDELGVRAISLNFAAFREVFSAAEXVGARPGARLGARLLTFASRFWQIEGLYXSNARYLPRWTPRYLCG",
    "response#2": "GLLLRAGQRDSLGYFATRHDKSVIFSPRGDGAVTYRLLAAVSLASADPIGPRSAWAGAIEAWLAETRRFGWFPAVLAASEEGARAYVTAGLKALPVGDEAIIEVAEFSLEGATMEPVRRAVRRVRRAGYTFTVARHGTLSAGELAEIERRAEDWRGDDTERGFSMALGRLGDPADEACVAVLARDEAGRLRGVLSLVPWGSHGLSLDLMRRDRGAENGLVEAMVAALVQAARDELGVRAISLNFAAFREVFSAAEXVGARPGARLGARLLTFASRFWQIEGLYXSNARYLPRWTPRYLCV",
    "response#3": "GLLLRAGQRDSLGYFATRHDKSVIFSPRGDGAVTYRLLAAVSLASADPIGPRSAWAGAIEAWLAETRRFGWFPAVLAASEEGARAYVTAGLKALPVGDEAIIEVAEFSLEGATMEPVRRAVRRVRRAGYTFTVARHGTLSAGELAEIERRAEDWRGDDTERGFSMALGRLGDPADEACVAVLARDEAGRLRGVLSLVPWGSHGLSLDLMRRDRGAENGLVEAMVAALVQAARDELGVRAISLNFAAFREVFSAAEXVGARPGARLGARLLTFASRFWQIEGLYXSNARYLPRWTPRYLCR"
  },
  {
    "reference": "MKVLGHGPYARMHPSGKLEGAANQVLAECWYNHGYMLSDTQKPDLYDLNREALGELLARWGAPRSLTLELWHALYRQRVASFEALPLPEALCAQLAAEVRWHIPHVVGRLEAPDGATRKDLLELADRQHIEVVLLRYRQRRSACISTQVGCACGCVFCATGGMGFVRQLTPGEIVAQVLHFQRELAAQGESLSNLVLMGMGEPFLNYDATVAALRQLLDPQGMGFPPRRITVSTAGIVPGIERFAVEGLGVNLAISLHAATDALREQLMPINRRYPLGMLFEAARAYAGHTGRRVMLEWALIAGVNDSPTQAEALVTLVEGLPAHINLILLNPTPGYAEQPATPSAVEAFIAVLDAAGIPHTMRQRRGGAIAAGCGQLRAQAK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have a high specificity for S-adenosyl-L-methionine binding, with minimal off-target effects.2. The protein with the reaction adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine, the S-methylcysteine intermediate must be optimized to ensure efficient catalysis.3. The protein must bind to [4Fe-4S] cluster in order to perform its enzymatic function.4. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.5. The designed protein should be involved in rRNA base methylation by localizing to cytoplasm and exhibiting 4 iron, 4 sulfur cluster binding, tRNA binding, rRNA (adenine-C2-)-methyltransferase activity, tRNA (adenine-C2-)-methyltransferase activity. The designed protein sequence is ",
    "response#1": "MNILNIDQNKLKQILVSNNFKAFSANQIFEWIYKKGILSFDEMTNLSKDLRDFLNKNFIIDQPKYRQQQIEKAIYGEFISDWSEAIALPEHLRKQLAEACPLDIHAEETISSGDSRKALIILEDELKIEAVLMMHKGDRNTVCVSSQVGCPMGCLFCATGKLGFKRNLTADEIVEQVLYFARQLKKENKRVTNIVFMGMGEPMVNYDNVMAAIRILNNPNGFNLGARRMSISTSGIPEGIRKFAREGLEVNLALSLHAPNNELRCKIMPVSRRHSLEEVMDALREYVDITHRKIMFEYIMIKDVTDTPELAHELVKLLSGWLGYVNLINYNQTEMFAPSTRERIEEFKQILEDGHITVVQRYTFGQDIDGACGQLAARTL",
    "response#2": "MNILNIDQNKLKQILVSNNFKAFSANQIFEWIYKKGILSFDEMTNLSKDLRDFLNKNFSGLRDLSFPELAVTLAAWGEAPFRARQVWEWAWRHLATKFDQMTNLPAGLRARLAESFTIGLPAVTARQADDLGTEKVLLSLADGQGVEAVLLRGGDRRTVCVSTQVGCPVGCPFCATGAMGFVRNLTPGEIAAQVLHFARELREAGEHVTHVVVMGMGEPLLNYDSTLKAIHNLNEGRGFRLGARRITVSTVGVVPGILRLAKEDLQVNLAISLHAPDDALRRKLVPLGERWPIAEVLAAADRYAHATGRRVSYEYVLLAGVNDHLDDARALAALLRGRLAHVNLIPFNPSPGLPFRAPAAGTVDAFRRALVESGVDATVRRSRGVAIQAGCGQLRVSSARNTVCVSSQVGCPMGCLFCATGKLGFKRNLTADEIVEQVLYFARQLKKENKRVTNIVFMGMGEPMVNYDNVMAAIRILNNPNGFNLGARRMSISTSGIPEGIRKFAREGLEVNLALSLHAPNNELRCKIMPVSRRHSLEEVMDALREYVDITHRKIMFEYIMIKDVTDTPELAHELVKLLSGWLGYVNLINYNQTEMFAPSTRERIEEFKYSDRATVCVSTQAGCAMACSFCATGLMGLNRNLTAGEILQQILHYERFLINTEDQKLTLGHKRVSNLVFMGMGEPLANYLNLWKAIRRLHDPDGINLSARKMTVSTVGLPQMIKRFGQEELPVNLALSLHAPNDTLRSQMMPISRRYPIAELIEACRDYIANTNRRLSFEYTLVEEVNDTVACARELGKLIKGMLCHVNLIPMNPVPGSSQRGSDINRVKVFQAELTRYGIPNSVRVEMGR",
    "response#3": "IDQPKYRQQQIEKAIYGEFISDWSEAIALPEHLRKQLAEACPLDIHAEETISSGDSRKALIILEDELKIEAVLMMHKGDRNTVCVSSQVGCPMGCLFCATGKLGFKRNLTADEIVEQVLYFARQLKKENKRVTNIVFMGMGEPMVNYDNVMAAIRILNNPNGFNLGARRMSISTSGIPEGIRKFAREGLEVNLALSLHAPNNELRCKIMPVSRRHSLEEVMDALREYVDITHRKIMFEYIMIKDVTDTPELAHELVKLLSGWLGYVNLINYNQTEMFAPSTRERIEEFKQILEDGHITVVQRYTFGQDIDGACGQLAARTL"
  },
  {
    "reference": "MPHTITTTDELAAYCARAATQPYVTVDTEFLRERTYFAQLCLVQVAMPGTDDTDAVLIDPLAEGLSLEPLYELFRNVNVVKVFHAARQDLEIFFVEGGLVPTPLFDTQVAAMVCGFGDQVGYETLVRRIAKANLDKSSRFTDWSRRPLSDAQKVYALADVTYLREIYEYLSAELARTDRTHWLEEELAQLTNADAYVVDPENAWKRLKLRSNSGRVVAIAQQLAAFRETYAQEKNVPRNRVLKDDALLELAGTKPKTVADLGKSRLLLRDARKGAIAEGLVAAVARGMSVPNSEIPKVQAGRDRSNLNPALADLLRVLLKAKAENAGVATKLIASSSDLDDIASGFTDGVWASGWRSEVFGADALRLLNGEVALAAKGQKVKIVEV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Initiates hydrolysis at the 3'-terminus of an RNA molecule and releases 5'-mononucleotides.2. The protein must exhibit nucleotide binding, 3'-5' exonuclease activity.3. The presence of HRDC domains is critical for the interaction of the designed protein with its intended targets.4. The presence of a divalent metal cation is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MSLITTTEALAEVCQRLASHAFVTVDTEFLRETTFWPKLCVVQLASADEAVAVDALASGLDLAPLFELMADPAVVKVFHAARQDLEIVWNLAKIIPAPLFDTQVAAMVCGFGDQVSYGDLVQTVCKVSLDKSSRFTDWSRRPLLPAQVDYAIADVTYLRDIYAILQDKLI",
    "response#2": "VMPNLYPFQERVASEVLSSIKKKVELKRPMYVTLHLACGFGKTITTCYLLSVHKKKAVICLPNKMLINQWKRAIESININHLVSVDGVGNLLKELVKKPADILIIVSRHLSNKEFCKKIHVDYDVFVLDESHMYNLMNNSTVTRFLTYYPPKICYFLTATPRRVNRIYCNDVINVSNSSDLKKYIKII",
    "response#3": "MSVIGSDEVGTGDYFGPMTVVCAYVDKTMLPLMKELGVKDSKDLKDPQIIEIARNLIKTIPYSLLVLKNEKYNSMQEKGMSQGKMKALLHNQAITHLLRKLDGVKPEAILIDQFAEPGVYFNHLKGRDIVKERTYFSTKAEGIHLAVAAASIIARYSFLMEMDKLSRAAGMTLPKGAGPHVDEAAAKLILKKGASALRTFTKLHFANTQKAQRLADL"
  },
  {
    "reference": "AMPHGVMGLITAGGEGVVAFADIRDLYVEDEAMEQGVASYIESLGAAFGSGFTNQISEKVTELTSMVTSMFTEKLLKSLIKLVSSLVIIVRNYEDTTTVLATLALLGCDVSPWAWLKKKVCDILEIPFVLKQGDSWMKKFTEACNAAKGLEWISNKISKFIEWIKNKVIPEAKDKVEFISKLKQLGMLENQINTIHQSCPSQEQQEILFNNVRWLAIQSRRFAPLYAAEAKRISKLENTINNYIQFKSKHRIEPVCVLIHGSPGTGKSVATSLMGRAIAEKENTSTYSLPPDPTHFDGYKQQGVVIMDDLNQNPDGADMKLFCQMVSTVEFIPPMASLEEKGILFTSDYVLASTNSSTITPPTVAHSDALSRRFAFDMEICPMSQYTVKGKLNMAEATRICKDCHPPANFKKCCPLVCGKAIQLMDKYTRQRFSIDELTTLVINEKVRRSNIGNCMEALFQGPSQYRDLKIDIKTSPPPECINDLLQAVDSQEVRDYCEKKGWIVNVSSDITIERNLNRAMTILQALTTFAAVAGVVYVMYRLFAGQQGPYTGLPAKKPNIPTIRTAKVQGPGFDYAVAMAKRNIVTATTSKGEFTMLGVHDNVAVLPTHASPGETIVIDGKEVEVLDAKALEDQAGTNLEITIXTLKRNEKFRDIRPHIPAQITETNDGVLIVNTSKYPNMYVPVGAVTEQGYLNLGGRQTARTLMYNFPTRAGQCGGIVTCTGKVIGMHVGGNGSHGFAAALKRSYFTQNQGEIQWMRPSKEVGYP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design must be focused on enhancing the efficiency of DNA-templated transcription, induction by virus of host autophagy.2. The protein should have a fast turnover rate for a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-   diphosphate + H(+) + phosphate. The designed protein sequence is ",
    "response#1": "MKVTDHILVPKHEIVPKEEVEEILKRYNIKIQQLPKIYEDDPVIQEIGAKEGDVVRVIRKSPTAGVSIAYRLK",
    "response#2": "VALAPHVGMGLDTRTQTWMSAEGAWRQVEKVETWALRHPGFTILALFLAHYIGTSLTQKVVIFILLMLVTPSL",
    "response#3": "MKVTDHILVPKHEIVPKEEVEEILKRYNIKIQQLPKIYEDDPVIQEIGAKEGDVVRVIRKSPTAGVSIAYRLK"
  },
  {
    "reference": "MTIPGTILDTARTQVLEQGIGLNQQQLMEVLTLPEEQIPDLMELAHQVRLKWCGEEIEVEGIISLKTGGCPEDCHFCSQSGLFESPVRSVWLDIPNLVEAAKQTAKTGATEFCIVAAVKGPDERLMTQLEEAVLAIHSEVEIEVAASIGTLNKEQVDRLAAAGVHRYNHNLETARSYFPEVVTTHTWEERRETLRLVAEAGMEVCSGGILGMGETLEQRAEFAVQLAELDPHEVPMNFLDPRPGTPFADRELMDSRDALRSIGAFRLAMPHTMLRFAGGRELTLGDKGSEQALLGGINAMIVGNYLTTLGRPMEDDLDMMDRLQLPIKVLNKVI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should incorporate a binding pocket that specifically recognizes [2Fe-2S] cluster to enhance enzymatic efficiency.2. The protein should be designed to have high stability and activity towards (4R,5S)-dethiobiotin + [sulfur carrier]-SH + 2 reduced [2Fe-   2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'-deoxyadenosine +   [sulfur carrier]-H + biotin + 2 L-methionine + 2 oxidized [2Fe-2S]-   [ferredoxin].3. Ideal protein should have a binding affinity for key enzymes in the Cofactor biosynthesis, biotin biosynthesis, biotin from 7,8- diaminononanoate: step 2/2 pathway to enhance the overall efficiency of the pathway.4. A protein with 2 iron, 2 sulfur cluster binding, iron ion binding, biotin synthase activity, 4 iron, 4 sulfur cluster binding is needed to enhance biotin biosynthetic process in a specific context.5. The protein contains novel Radical SAM core domains that confer a unique function or activity.6. The [4Fe-4S] cluster, [2Fe-2S] cluster binding site should be stable and able to withstand changes in environmental conditions.7. For general function, the protein need meet that Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism. The designed protein sequence is ",
    "response#1": "FFGTSVHLCVIRNGKSGRCSEDCAFCSQSVRAKADIDAYPLISEIDLAEPGRRLESTPVNRYSVVTSGRGLSSAEVSTVSRALAAIDTTAVSTCASLGILDPGQLAMLRDAGVTRYHHNLESAESLFANVCTTHTYEERVQTVKAAKAAGLSVCCGGIFGLGETDDQVAELAFALRDLDVDSVPVNFLVPIKGTAMEKASRLTPLRCLRIIAFLRHVLPDKEIIVCGGDPVNALVPVEGTPLEDQPRVSVWEMVRMIATARIIMPKAMVRLSAGRVRMNTEEQALCFLAGANSIFAGDKLLTTPNPEVNADKEMFQVLNLKWLHQLADRIISGYRITKVEALALTEIEGQDQILLLCEAADRIRQACCGNRVDLCSIINIKSGHCSENCSFCSQSVHHPGQDSPVYGLKTSEEIVQQAKAAAAAGAKRFCLVSQGRGLKYNSPKSKEFAEILATVKRITTEAKIKPCCALGELTLEQAQALKEAGVTRYNHNLEASATFYPQIVTTHTWADRVETVKNLKAAGIQACTGGIIGMGESWEDRIDLALSLRDLEVDSVPINLLNPRQGTPLGHLPKLDPFEALQAIAIFRFILPQQILRYAGGREAIMGELQSLGLKAGINAMLIGHYLTTLGQSPQQDQAMLKSLGLPVNFLLPFEGTPLAGGAQHITPQWCLKRLAMVRFAHPDSEVRAAAGREQHIRTMQPLALEVVNSIFLGDYLTSEGAAGAADLQMIEDGGFIE",
    "response#2": "SPAAIRHDWTLAEIQAIHDMPLLDLVHRAGVVHRAHNDPADIQRAALLSIKTGGCPEDCAYCPQSAHHKGANLPRERLMPVDAVLKEAAAAKANGAHRFCMGAAWRKPKDGPDFDAVLEMVRGVRGLGMEACVTLGMLTQSQAQRLAEAGLTSYNHNLDTGPEFYGDIISTRTYDDRLQTLEHVRQAGIGVCCGGIVGMGERVRDRAEMLLVLANHAPHPESVPINALVAVEGTPLEDRPPIDPLDLVRMCATARIVMPKARVRLSAGRKSLTREAQILCFLAGANSIFYGERLLTTANNEADADAELLRDIGVPVESAFEPLKAADIQRAALLSIKTGGCPEDCAYCPQSAHHKGANLPRERLMPVDAVLKEAAAAKANGAHRFCMGAAWRKPKDGPDFDAVLEMVRGVRGLGMEACVTLGMLTQSQAQRLAEAGLTSYNHNLDTGPEFYGDIISTRTYDDRLQTLEHVRQAGIGVCCGGIVGMGERVRDRAEMLLVLANHAPHPESVPINALVAVEGTPLEDRPPIDPLDLVRMCATARIVMPKARVRLSAGQETSLTPINYLVQVEGTPLYGIPELDPFEFVRSIAAARITMPRAKVRLSAGRRQMSEAIQALCFLAGANSIFYGDKLLTTGNPDTEQDAALLEKLGLHEARLAV",
    "response#3": "WLHQLADRIISGYRITKVEALALTEIEGQDQILLLCEAADRIRQACCGNRVDLCSIINIKSGHCSENCSFCSQSVHHPGQDSPVYGLKTSEEIVQQAKAAAAAGAKRFCLVSQGRGLKYNSPKSKEFAEILATVKRITTEAKIKPCCALGELTLEQAQALKEAGVTRYNHNLEASATFYPQIVTTHTWADRVETVKNLKAAGIQACTGGIIGMGESWEDRIDLALSLRDLEVDSVPINLLNPRQGTPLGHLPKLDPFEALQAIAIFRFILPQQILRYAGGREAIMGELQSLGLKAGINAMLIGHYLTTLGQSPQQDQAMLKSLGLPVNFLLPFEGTPLAGGAQHITPQWCLKRLAMVRFAHPDSEVRAAAGREQHIRTMQPLALEVVNSIFLGDYLTSEGAAGAADLQMIEDGGFID"
  },
  {
    "reference": "MKKIKCALIGPGNIGTDLLAKLKRSSVLEPVWMVGIDPESEGLKRARELGVKTTAEGVDGLLPHVLADGVQIAFDATSAYVHAENARKLNALGVMMIDLTPAAIGPYCVPPVNLKEHLGKREMNVNMVTCGGQATIPMVAAVSRVQPVAYAEIVATVSSRSVGPGTRKNIDEFTRTTAGAVEKVGGARKGKAIIIINPAEPPLMMRDTIHCLTETEPDQQRITESIHAMIEEVQKYVPGYRLVNGPVFDGKRVTVFMEVAGLGDYLPTYAGNLDIMTAAAARTAEMFAEEMIAGNLTLEPVVA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The NAD(+) binding site should be stable and able to withstand changes in environmental conditions.2. The design of the protein must focus on the precise arrangement of amino acids in the Acyl-thioester intermediate to enable effective catalysis of acetaldehyde + CoA + NAD(+) = acetyl-CoA + H(+) + NADH.3. A protein with acetaldehyde dehydrogenase (acetylating) activity, NAD binding is needed to enhance aromatic compound catabolic process in a specific context. The designed protein sequence is ",
    "response#1": "CGGQATIPIVAAVSRVTPVAYAEIVASIASKSAGPGTRANIDEFTETTSHAIETVGGARRGKAIIVLNPADPPLIMRDTVLCLISARDPATHDAIRNSIQAMVEHVATYVPGYRLKQQVQITPVPDGQPVHTLLASGDTAAPTHQVSVFLEVEGAAHYLCGGQATIPMVAAVSRVQPVAYGEIVATVSSRSIGPGTRKNIDEFTRTTAGAIEQVGGAKEGKAIIVVNPAEPPLMMRDTIHCLTETEPDQDAITASVHAMIAEVQKYVPGYRLKNGPVFDGNRVSIFMEVEGLGDYLYVVPCGGQATIPMVAAVSRVQPVAYGEIVATVSSRSIGPGTRKNIDEFTRTTAGAIEQVGGAKEGKAIIVVNPAEPPLMMRDTIHCLTETEPDQDAITASVHAMIAEVQKYVPGYRLKNGPVFDGNRVSIFMEVEGLGDYLYVVPMTVDKT",
    "response#2": "CGGQATIPLVYAVSRVANVKYAEMVSTVSSSSAGPGTRQNIDEFTFTTSRGLEVIGGAEKGKAIIILNPAKPPILMRNTVYIAYEDGDDHQIRHSIGQMIHDVQQYVPGYRLKGEPIFDRRETPKGRLDVVILLLEVEGAGDFLG",
    "response#3": "CGGQATIPIVAAVSRVTPVAYAEIVASIASKSAGPGTRANIDEFTETTSHAIETVGGARRGKAIIVLNPADPPLIMRDTVLCLISARDPATHDAIRNSIQAMVEHVATYVPGYRLKQQVQITPVPDGQPVHTLLASGDTAAPTHQVSVFLEVEGAAHYLCGGQATIPMVAAVSRVQPVAYGEIVATVSSRSIGPGTRKNIDEFTRTTAGAIEQVGGAKEGKAIIVVNPAEPPLMMRDTIHCLTETEPDQDAITASVHAMIAEVQKYVPGYRLKNGPVFDGNRVSIFMEVEGLGDYLY"
  },
  {
    "reference": "MGQLTAVILAAGEAKRMRSRQPKVLHPLCGRPLIAYALRTARAVADRTVVVVGPNAADVRATAGDGVLFVEQRERLGTGHALQQARAACGEGPVVVLAADMPLVSSETLERLIDHHRATSAAATLLTVVAGRPQGYGRILRQGGRVKRIVEDRDATDDQKKITEINVSVYCFDTRRLWKALAEVRPDNEQGEYYVTDVIGILAKAGGRIEAVVAPDPVEGLQVNDRKQLAAVAAILRRRILDRLMTDGVTIVDPAATYVEDTVVIGADTTVYPHVVIEGPTSVGSECVIGSGCQISGSRLADRVHLRPYCVLTEATVEEGAQLGPFCHLRPLSHVGPRAKIGNFVEMKKSRIGPGSKVPHLSYVGDATVGEGVNIGAGTITCNYDGVAKHETRIGDGVFVGTNSSLVAPLTIGEGAYIAAGSTITKDVPPGALAVGRAHQIVKEGWAARKAKKRTGVED",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The ideal protein would be one that plays a critical role in UDP-N-acetylglucosamine biosynthetic process, cell morphogenesis through magnesium ion binding, glucosamine-1-phosphate N-acetyltransferase activity within cytoplasm.2. The protein should be optimized for activity in Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D- glucosamine biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The presence of MobA-like NTP transferase domains is critical for the interaction of the designed protein with its intended targets.4. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain. The designed protein sequence is ",
    "response#1": "MKLAIILAAGLGTRMKSRTPKVLHPLCGRPMLAYVLEAWADVTPELPAAPPVVVYSPPVAAVRDAGLGEAVRFALQDEPRGTADAVRAGLAEIPEDTAEILVLSGDVPLVLGPQLLAILEQRRLDDAAIALAMRFEDPSGYGRVVRDGAGAVRAIVEERDATAAQRAITEVNAGVYAFDAAVLRDALGRLSTDNAQGEKYLTDVLGLARADGHAVAALTAGDHWLVEGANDRVQLAALGAELNRRTVERWQRAGAKVGEGALIGPYARLRPGADIGRHVHIGNFVEVKGGATVVDPDTTWIGVEVEIGRDVVIHPNTQLWGSTVIGDGAEIGPDTTLTDMEVGEGATVVRTQGELSVIGARASVGPFTYIRPGTVLGEDGKLGGFVESKNATIGPGSKVPHLTYIGDATVGRESNIGASSVFANYDGVSKHHTTIGDYCRTGSDTMFVAPVTVGDGAYTGAGTVVTQDVPAGALAIKEGRQRNIEGWVARKRPGSAAARAAERALAAQEPVDTAPAQGPTE",
    "response#2": "MTAILAAGRGTRMKSNLPKVLHSLGGRSLIGRVIDSVKPLSPTRQIVVVGYQAVEVQAAMESIPDLEFVEQTVQLGTGHAIQQLLPHLEGYTGDLLVLYGDVPLLRTETLKNLLQTHTQHQNAATILTLLTDTLPDPRGYGRVLRDADDAVRAIVEEKDATDAQRAIREINTGILVAPTAALKTWLASLRNDNAQGEYYLTDIVAMAVAEGLAVRTTQPEHAWETLGVNSKAQLAELERAHQRDCAQRLLEQCGV",
    "response#3": "VIVLAAGAGTRMKSRTPKILHPVGGISMLGHALAAASALHPRFLAVVVRHERDIVAAHAAGHAPAAVIVDQDEVPGTGRAVQVAVSALDAVSTLEGTVVVTYGDAPLLEADTLRSLVAVHETDGNAVTVLTATVLDDPRGYGRIVRDASGAVTAIVEQKDADPAQKAVREINSGVFAFDGRLLADALGKVRTDNSQGEEYLTDVLGILRAAGHRVGASVAGDHREILGINNRVQLAEARRILNDRLLAAAMLAGATVVDPDTTWIGVEVEIGRDVVIHPNTQLWGSTVIGDGAEIGPDTTLTDMEVGEGATVVRTQGELSVIGARASVGPFTYIRPGTVLGEDGKLGGFVESKNATIGPGSKVPHLTYIGDATVGRESNIGASSVFANYDGVSKHHTTIGDYCRTGSDTMFVAPVTVGDGAYTGAGTVVTQDVPAGALAIKEGRQRNIEGWVARRRPGSAAAQAAQAAQADQSEQKDKQDQG"
  },
  {
    "reference": "MEFDSIESALNALKAGDPIVVLDDENRENEGDLIAITEWMKDDTINFMAKYGRGLICAPISKSIAVQAGLSPMVAMNTDPHGTAFTTSIDHVSTSTGISAYERTQTIRALIQENMNTQSFNRPGHVFPLIAQENGVLARRGHTEAAIDLAMLTGAKPAGVICEIMNEDGTMARGEDLQAFKEKHQLKMITIEALVSYIKVHRKLIKQEAKVKLPTRYGSFDMIGFTSEFDGQEHLAIIKGDIHPEMNVRIHSACATGDIFHSERCDCGEQLEKAMAYIQKNGGIILYLPQEGRGIGLMNKLKAYELIEKGYNTITANEALGFEPDLRDYDIAAQILKHLGVSSVHLLSNNPDKFQGLKKYDIDIKGRIPLITNENENNHDYLEVKKTQMGHLI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be able to bind GTP ligand in a variety of conditions2. The Proton acceptor; for GTP cyclohydrolase activity active site of the designed protein must be optimized for substrate binding and specificity.3. The designed protein should contain one or more GTP cyclohydrolase II domains that are essential for its function.4. The designed protein should interact with enzymes and substrates within the riboflavin biosynthesis pathway to enhance or regulate its activity.5. The designed protein should have GTP binding to facilitate riboflavin biosynthetic process. The designed protein sequence is ",
    "response#1": "SICLPTDYGEFKLIAYESATSPEPHLALCKGGVGDLDAKGQPIEHEGPVLIRVHSECMTGDLFHSQRCECGYQLITALKMIEKEGKGALIYLRQEGRGIGLSNKLRAYKLQEQGLDTVDANLKLGFVADRRDYGIGAQICRDLGLRNVRLLTNNPKKVSRLEVYGIRIAEQIAVKE",
    "response#2": "GVEMPTEFGDFDLIAFRQTNTDEIHMALVKGEWKEGEPILVRVHSSCVTGDIFGSCRCDCGPQLHSAMEMVNQAGKGVVLYMNQEGRGIGLINKLKAYKLQEEGMDTVQANQALGFPMDKRDYGVGAQILRDLGVSKIKLLTNNPTKRAGLLGYGLEIVETE",
    "response#3": "SICLPTDYGEFKLIAYESATSPEPHLALCKGGVGDLDAKGQPIEHEGPVLIRVHSECMTGDLFHSQRCECGYQLITALKMIEKEGKGALIYLRQEGRGIGLSNKLRAYKLQEQGLDTVDANLKLGFVADRRDYGIGAQICRDLGLRNVRLLTNNPKKVSRLEVYGIRIAEQIK"
  },
  {
    "reference": "MRVMGTQRNCQRWWTWGILGFWMLMTCSVMGNLWVTVYYGVPVWKEAKTTLFCASDAKSYEPEVHNVWATHACVPTDPSPQELVMENVTENFNMWKNDMVEQMHTDIISLWDQSLKPCVKLTPLCVTLDCIEPNNSTRNITIDQEMKEEVKNCSYNMTTELRDKKQKVHSLFYRVDIVPLDEGNTSYRLINCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNNETFSGKGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAKEEIMIRSENFTNNAKNIIVQLKRPVEINCTRPNNNTRRSVRIGPGQAFYTNDIIGNIRRAYCLVNKTDWNKTLHEVATQLRKHFGNKTIIFNNSTGGDLEITTHSFNCGGEFFYCNTSGLFNSTWYTNTSVQEPNSTESNNITHITLHCRIKQIINMWQRAGQAMYAPPIQGEIRCVSNITGLILTRDGGDHNNSDETFRPGGGDMRDNWRSELYKYKVVKIEPIGIAPTKARRRVVQREKRAIGMGAVFLGFLGAAGSTMGAASVALTVQAKQLLSGIVQQQNNLLRAIEAQQHMLKLTVWGIKQLQARVLALERYLGDQQLLGIWGCSGKLICTTNVPWNDSWSNKSQTEIWNNMTWLQWDREISNYTQIVYSLLEQSQYQQEKNEQELLELDKWANLWNWFDISKWLWYIKIFIMIVGGLIGLRIVFAVLSIINRVRQGYSPLSFQTLTPSPGGLDRPGRIEEEGGEQDRDRSIRLVSGFLALAWDDLRSLCLFSYHRLRDF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of Retroviral envelope protein GP41-like domains is critical for the interaction of the designed protein with its intended targets.2. The designed protein should be involved in positive regulation of plasma membrane raft polarization, fusion of virus membrane with host endosome membrane by localizing to host cell plasma membrane, virion membrane, host cell endosome membrane, viral envelope and exhibiting structural molecule activity.3. The protein must exhibit the following characteristics: Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane.4. The protein design should include a Di-leucine internalization motif, YXXL motif; contains endocytosis signal motif to ensure proper function. The designed protein sequence is ",
    "response#1": "MKVKGIQMNWPHLWKWGTLILGLVIICSASDNLWVTVYYGVPVWKEATTTLFCASDAKSYDTEVHNVWATHACVPTDPSPQEIRMENVTENFNAWKNNMVDQMHEDIISLWDESLKPCVKLTPFCVTLNCTKVNKTNPNTTTNTNSSFMKEEVKNCSFNTTTELRDKKQKAYALFYKLDIVQMNDNRTYRLINCNTSVIKQACPKISFDPIPIHYCAPAGYAIFKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKNIIVHLNESVEINCTRPFNNTRTSIRIGPGQMFYRTGEITGDIREAYCEINRTRWNKTLSQVAKKLKEHFNKTIIFQPPSGGDLEITTHHFNCRGEFFYCNTTKLFNSTWNETGMSNDTITLPCRIKQIINMWQGVGQAMYAPPISGRINCVSNITGILLTRDGGNSSSKNETFRPGGGNIKDNWRSELYKYKVVQIEPLGVAPTKAKRRVVEREKRAVGIGAVIFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLRDQQLLGLWGCSGKLICTTSVPWNSNWSNKSEKEIWDNMTWMQWDREIDNYTNAIYRLLEESQNQQEKNEKDLLALDSWNNLWNWFNISNWLWYIKIFIMIVGGLIGLRIIFGVLSIVKRVRQGYSPLSFQTLTHHQREPDRPERIEEGGGEQGRDRS",
    "response#2": "MRVKGYXRNYQHLWTWGTMLLGMLMICSAAGNLWVTVYYGVPVWRDAETTLFCASDAKAFEKEVHNVWATHACVPTDPNPQEIALENVTENFNMWKNNMVEQMQTDIISLWDQSLKPCVKLTPLCVTLNCAEPSNNRSANNLWVTVYYGVPVWKDAKTTLFCASDAKAYKTEVHNVWATHACVPTDPNPQEIDLENVTEMFNMWKNSMVEQMHTDIISLWDQSLKPCVKLTPLCVTLHCANVNSSHDSSTVTNGNSTVPNVNSTVTEQEMKNCSFNTTTEIRDRKQKAYSLFYKLDIVPISNNNREYILMNCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGLCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIMIRSENISDNAKTIIVQLITPVPINCTRPNNNKRKSIHIGPGQAFYATEDIIGNIRQAYCTVSKSLWNTTLKQVADKLKEHWNKTIIFTEPSGGDLEITTHSFNCGGEFFYCNTTGLFNSTWNTADLFNNTEPNNETITLPCKIKQIINVWQRVGQAIYAPPIEGLIKYNSNITGLLLTRDGGSHNSSNRTETFRPGGGNMKDNWRSELYKYKVVKIEPLGVAPTKAKRRVVERGKRAAIGALFLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNKSQSEIWNNMTWMQWDREVINYTNMIYDLLEESQNQQEKNEQDLLALDKWASLWNWFDISHWLWYIKIFIMIVGSLIGLRIVFAVLSIINRVRQGYSPLSLQT",
    "response#3": "MRVKGYXRNWLQWWIWSILGFWMIMMCNDKGKLWVTVYYGVPVWKEATTTLFCASDAKSYDTEVHNVWATHACVPTDPSPQEIRMENVTENFNAWKNNMVDQMHEDIISLWDESLKPCVKLTPFCVTLNCTKVNKTNPNTTTTNNGGVGMMNEEIKNCSFNVTTNEGNKVKKEYALLYKLDVVSIDGSSSKNETYSNYRLISCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNDKKFNGKGECKNVSTVQCTHGIRPVVSTHLLLNGSLAEEEVVIRSDNFSNNAKTIIVQLNKTVKINCTRPNNNTRRSISFGPGRAWYATTDIVGDIRQAHCNISTTEWNNTLRLVVNKLQEQYGTNKTIRFEQPVQGGDLEIVMHSFNCGGEFFYCNTSKLFNSIWNNASTWNDTEGNGTLTLPCKIRQIINMWQKVGKAMYAPPISGRINCLSNITGLLLMRDGGSNDTNEPEIFRPGGGDMRDNWRSELYKYKVIKIEPLGIAPTKAKRRVVQKEKRAVGIGAVFLGFLAAAGSTMGAASITLTVQARTLLSGLVQQQNNLLRALEAQHHLLQLSVWGIKQLQAKVLAIERFLRDQQILGLWGCSGKXICYTTVPWNETWSSNTSYDTIWNNLTWQEWDKKVRNYSGVIFNLIEQAQEQQNTNERSLLELDQWASLWTWFDLTNWLWYIKIAIMIVAGIIGIRIISVIITIIARVRQGYSPLSLQT"
  },
  {
    "reference": "MSGGLDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYIYKRKSDGIYIINLKRTWEKLLLAARAIVAIENPADVCVISSRNTGQRAVLKFASASGATPIAGRFTPGTFTNQIQAAFREPRLLVVTDPRADHQPLTEASYVNIPTIALCNTDSPLRYVDIAIPCNNKGAHSVGLMWWMLAREVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKATTKEEYQGEWTAPVAEFPQAEVADWSEGVQVPSVPIQQFPAERPEIPAAKPAAEDWSSQPASTDDWSAAPTAQASEWTGTTTEWS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that May play a role in cell fate determination and tissue morphogenesis.; Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways; Required for the processing of the 20S rRNA- precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits; Required for the assembly and/or stability of the 40S ribosomal subunit; Also functions as a cell surface receptor for laminin2. Target a Polar residues compositional bias in the protein's composition for improved properties.3. A protein that localizes to plasma membrane, nucleus, cytosolic small ribosomal subunit and can perform laminin receptor activity, structural constituent of ribosome, laminin binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "YFYRDPEEIEKEEQAAAEKAVGKEEFQGEWTAPVPDFNQPEVADWSEGVQVPSVPIQQFPAGIEAPGKPAPAEVYAEDWSAQPATEDWSAAPTAQAGDWGGATADWR",
    "response#2": "YFYRDPEEIEKEEQAAAEKAVTKEEFQGEWTAPAPEFTAAQPEVADWSEGVQVPSVPIQQFPTEDWSAQPATEDWSAAPTAQATEWVGATTEWR",
    "response#3": "YFYRDPEEIEKEEQAAAEKATTKEEFQGEWTAPVAEFPQAEVADWSEGVQVPSVPIQQFTAERTDVPPAPKPTEDWSTQPASTDDWSAAPTAQASEWTGTTTEWR"
  },
  {
    "reference": "MSEMQTREMLIGEIKLFNKWSYDFIEVRDPSLKKYICLKPVYLPHSGGRHEHKRFGKALVPIVERLINNVMRPGRNMGKKHLAYNIVKKSFELIYLKTGENPLQVLVRAIENAAPREETTRIMYGGITYHVSVDIAPLRRIDLALRHLTEGARLKAFHNPISIEEALAEEIALAASNDPKSYAVQKKEEIERIALSSR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Is located at the subunit interface close to the decoding center..2. The protein should have a specific function in translation and should be localized to small ribosomal subunit in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "RKHSAPIRKVLADPVFNSVLVTKLINAIMLDGKKSLAQDILYSAFNLVKEKTQKDPMEVFQAAIDNVTPQLEVRTRRIGGTNYQVPTEVSARRKQTLALRWLVQYARLRNEKTMDVRLANEIIDASNKTGGAIKKREDTHKMAEANF",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#3": "GRKHSAPIRKVLADPVFNSVLVTKLINAIMLDGKKSLAQDILYSAFNLVKEKTQKDPMEVFQAAIDNVTPQLEVRTRRIGGTNYQVPTEVSARRKQTLALRWLVQYARLRNEKTMDVRLANEIIDASNKTGGAIKKREDTHKMAEANF"
  },
  {
    "reference": "MNATSKHMNSLTALLEGLVADLSLIEDISVADISIDSRKVKKCSLFLSLVKDEKQRFQYLVQALNGGAAIVLFDAQQTLSSQEKLAIEKSKVKAYFIADLSDKAGEIAARFYDQPSQELTVMAVTGTNGKTSVSQFIAQSLESLGLACGIVGTLGVGRIGDLRLSGMTTPDPVSLQAVLADFHHQAIKYVVIEASSHALQQGRLNSVAIDVAVLTNLSRDHLDYHHSMSDYALAKQRLFEFTSVKIAVINNNDTFGKQLIETLATADIELITYGCEIDSITKQNTTLNAEDIQTTTQNISFIVDSKWGQANINSQLIGRFNVDNLLAAFGSLLAIGIAFNQAGKAIQECQSVDGRMQSYGGGQQPQVVVDFAHTPDALAQALQALRPHVLADGQLWCVFGCGGDRDQGKRPLMAASVEAYADQVVVTDDNPRSESSVAIVEDILAGFKLPEQVHIEHDRLLAITYAVTHATTLDIVLIAGKGHEQYQEISGVRHLYSDVEAVNIALQAANDEHHSFVGVK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.2. The protein should have a fast turnover rate for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.3. The protein design should consider the endogenous proteins and enzymes present in the Cell wall biogenesis, peptidoglycan biosynthesis pathway to avoid interference with native metabolic processes.4. The presence of Mur ligase C-terminal domains is critical for the interaction of the designed protein with its intended targets.5. The designed protein should be involved in peptidoglycan biosynthetic process, regulation of cell shape, cell division by localizing to cytoplasm and exhibiting ATP binding, magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity. The designed protein sequence is ",
    "response#1": "MKLQDILKNVDIKSVTGNVDIVTGFAIDHRKVAPGTIFGAFQGMQVNGEDFVPAAIEAGAIAVVARPSVAVVTEIVNDSRKAREGVVFVCIKGAVADGHKYIPDVLKKNVSAIIVEDEVDLKAYGTDADGVTFIKTDDTRIAMARMAAVYYGRPSAAMKLVGITGTNGKTTTSQLLAQMLSVSDKPCGVIGTLGAGYMDRLEETGHTTPEAVELQRLLAEMADAGCKYAVMEVSSHALDQGRVNGTDFRTAIFTNLTQDHLDYHKTMEAYFAAKKRLFTDYLVNGATKPAVAVLNADDPYSARMAEGAAAQVLTYGLGEDADLQAVGMELSSKGTDFTLTTMAGERTVRISLLGKFNVYNALAALGAGVAEGISLETVAAGLENCAGVPGRFEVVRAANGTTAVVDYAHTPDALENVLRTIEEIRTPDQQLIVVCGCGGDRDRTKRPEMARIAVKYATTAIFTSDNPRHESPEAILDEMTAGLDAGTRCVRITDRAEAIRTAVLLSRPGDILLVAGKGHETYQIIGKEKHHFDDREAAAEAIRELQG",
    "response#2": "MKLKELLTNIKIDSIAGNNEDCVTGFAIDHRKVAPGTIFGAFQGMQVNGEDFVPAAIEAGAIAVVARPSVAVIVPDVRKALSIVSANFFDHPAKKLKLIGITGTNGKTTTVTLLYDLVRALGYRAGLISTVVYKIDGREIESTHTTPDPIRLNAMMREMTDAGCEFCFMECSSHAIVQERTRGLDFAGGIFSNITHDHLDYHKTFAEYIRAKKRFFDALPKGAFALTNADDRNGAVMVQNTAAAVSTYSLREMADFRCRILEMHLDGMLLRIDGQEVWTGLLGRFNAYNLLAVYGAASLALGIDKEAIAKGISALKNVPGRFEVVRAANGTTAVVDYAHTPDALENVLRTIEEIRTPDQQLIVVCGCGGDRDRTKRPEMARIAVKYATTAIFTSDNPRHESPEAILDEMTAGLDAGTRCVRITDRAEAIRTAVLLSRPGDILLVAGKGHEDYQEIKGVKHHFDDREEAKKAL",
    "response#3": "MTVTEIVNDSRKAREGVVFVCIKGAVADGHKYIPDVLKKNVSAIIVEDEVDLKAYGTDADGVTFIKTDDTRIAMARMAAVYASWFFGEPSRRLKVVGITGTNGKTTTSQLLAQMLSVSDAKAGLLGTIETIIGDEKLPASHTTPESLELQRLFREMVDAGCTHVVMEVSSHALALDRVAGCRFDAGVFTNLTQDHLDFHGDMESYFQAKKQLFTMLDDKAFAVVNLDDPWGKRMAGQCRARVITFGIDNPADLRAARVVLGADGVSFDLVTAEGRVALRLSMPGRFNVYNALAAAACALAEGVPLESIAGGLGALEGVPGRVEMVKVSDDFILLIDYAHNAMALSSILTTLREYHPGRLVCVFGCGGNRARSRRYEMGEVSGRMADFTIITSDNPRYENPEAIMDDIEVGMKKTTGKYIRIADRKEAIAYAIHHGKPGDIIVLAGKGHETYQIIGKEIKRLITDSRACQAGDLFIGMTGTQVDGGKFAPMAIAQGAIAVIISQQAFAELATELQTRAIAVDDVVLACSKLATAFRQTRGSR"
  },
  {
    "reference": "MVRDSFDGGHTGDPERDLAYEREHVRRDTMSDEVVPDDVAQYLIYFKRMIDEENVVEIHNLYEHGFPDLTERYFQQRLWPNEEAVENIVGSGNRIVAFSDSRIFIILYKELYFRHVYTRMQRGPSLAHRFDSYQNYQELFCEVLTPEKQPLSLQLPNVWLWDIIDEFVYQPFCLYKANPGKRSPEEYEDLLSIEQNQSAWNIYPVLNILYSLLAKSQIDEQLLAIREGRNPDDVADDFGRSALYFKLGYFSLIGLLRTHVLLGDYHQALKTVENLELDPKGLYNTVPSCLVTFHYFVGFSHMMMRNYGEATKIFVNCLLYIQRTKSVQQQNQQQKKNFQYDVIGKTNEQLYHLLAICLTLQPQRIDDSIQSQLYERTGERMNHMSNGNIDEFRLAFQQGCPKFLSPTTVVYEGPNQAKEPLLRQCNAFLEEIESQIMLPILRGYLKLYTTLPTRKLASFMDVSDADYDSFVGKLLSFKMIVNELGKECMDRCEIDDSTTDLDFYVDKDMIIIADTKVARRIGEYFIKQIQKLQEVNRKLKELPVIPAVSS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should contain one or more PCI domains that are essential for its function.2. The protein must exhibit the following characteristics: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The designed protein sequence is ",
    "response#1": "MQRMSTRVLLATLSIPITPERTDIARLLDMDGIILEKQRRLATLLGLQAPPTRVGLINDMVRFNMLQYVVPEVKELYNWLEMDFHPLKLCTRVTKVLDWVKEQAEKEPELQQYVPQLQSNTILRLLQQVAQLYQTIEFSRLASLVPFVDAFLLERAIVDAARHCDLQVRIDHSSRTLSFGSDLL",
    "response#2": "MQRMSTRVLLATLSIPITPERTDIARLLDMDGIILEKQRRLATLLGLQAPPTRVGLINDMVRFNMLQYVVPEVKELYNWLEMDFHPLKLCTRVTKVLDWVKEQAEKEPELQQYVPQLQSNTILRLLQQVAQLYQTIEFSRLASLVPFVDAFLLERAIVDAARHCDLQVRIDHSSRTLSFGSDLI",
    "response#3": "MQRMSTRVLLATLSIPITPERTDIARLLDMDGIILEKQRRLATLLGLQAPPTRVGLINDMVRFNMLQYVVPEVKELYNWLEMDFHPLKLCTRVTKVLDWVKEQAEKEPELQQYVPQLQSNTILRLLQQVAQLYQTIEFSRLASLVPFVDAFLLERAIVDAARHCDLQVRIDHSSRTLSFGSDLL"
  },
  {
    "reference": "MRNNEKQELLVKEFKAILKAERFGSQGEIVDALRQSGFDNINQSKVSRMLTKFGAVRTRNAKMEMVYCLPVELGVPTVSSSLRELVMDIDYNNALVVIHTGPGAAQLIARLLDSLGKAEGILGVVAGDDTIFITPTRNIEVAELFDSVCDLFEYSV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The ideal protein would be one that plays a critical role in protein complex oligomerization through arginine binding, DNA binding, DNA-binding transcription factor activity within cytoplasm.2. For general function, the protein need meet that Regulates arginine biosynthesis genes.3. Ideal protein should have a binding affinity for key enzymes in the L-arginine biosynthesis [regulation] pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MNKALRQKKIRELVENNDVSGQQELLGMLEKEGFSVAQPTLSRDFAEMGIVRNRTADGGYRLSVPEE",
    "response#2": "MNKALRQKKIRELVENNDVSGQQELLGMLEKEGFSVAQPTLSRDFAEMGIVRNRTADGGYRLSVPED",
    "response#3": "MNKALRQKKIRELVENNDVSGQQELLGMLEKEGFSVAQPTLSRDFAEMGIVRNRTADGGYRLSVPES"
  },
  {
    "reference": "MMRSHYCGTLNRSHVGQAVTLSGWVHRIRNLGRFIFMQIRDREGIVQVFFDEKDDTLFKVASQLRAEACVQIQGQVIARDEAQINPEMATGEIEVLVQNVLVYNNAEVLPLDFNQNNTEEQRLKYRYLDLRRHKMAENLKTRAKITSFVRRYMDEHGFLDIETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFLTAEEVRALMENMIHSLWLDRLNVDLGKFPIMSWQEAMQHFGSDKPDLRNPLELVDIADIVKNVEFNVFNEPANAIDGRVTVLRVPNGATLTRKQIDEYTQFVAIYGAKGLAWAKINDINAGMDGIQSPVAKFLNAEIFNALIERTAAQNGDILFFGADKWQVVTDAMGALRLKIGRDLALTDQTAWKPLWVIDFPMFERDGEGNLSAMHHPFTAPKDLSPDQLAADPIKAVANAYDMVINGYEVGGGSVRIFDPKMQQTVFNILGINEQDQQEKFGFLLDALKFGTPPHAGLAFGLDRLTMLITGTENIRDVIAFPKTTAAACLMTEAPSYANPQVLQELAIQTTVETE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp- AMP and then transferred to the acceptor end of tRNA(Asp).2. A protein that contributes to aspartyl-tRNA aminoacylation by functioning in cytoplasm with ATP binding, aspartate-tRNA ligase activity, nucleic acid binding.3. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP, L-aspartate binding.4. We need a protein that can perform ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-   aspartyl-tRNA(Asp) with high efficiency. The designed protein sequence is ",
    "response#1": "MRMRSEVNRIARDVLHGRDFVEVETPTMTRSTPEGARDFLIPARLQPGNWYALPQSPQLFKQLLMVGGLERYFQIARCYRDEDFRADRQPEFTQLDIEMSFVDQDDVIELGEEIISALWRETAGHEIPRPIQRMTYADAMARFGTDKPDLRFGLELTEMTEYFADTPFRVFRAPYVGAVVMPGGADQPRRQLDAWQDWAKQRGAKGLAYVLVGQDGTLSGPVAKNLSDGERDGLVKAVGAAPGDCVFFAAGDRSSSRALLGAARLEIGERCGLIDHDAWSFVWVVDAPMFESIDDTDDVAVGSGRWTAVHHPFTSPNADWVDNFESDPANALAWAYDIVCNGNEIGGGSIRIHRSDVQKRVFELLGISEEQAQDKFGFLLEAFKYGPPPHGGIAFGWDRICMLLAGADSLRDVIAFPVKTGMK",
    "response#2": "MRMRSEVNRIARDVLHGRDFVEVETPTMTRSTPEGARDFLIPARLQPGNWYALPQSPQLFKQLLMVGGLERYFQIARCYRDEDFRADRQPEFTQLDIEMSFVDQDDVIELGEEIISALWRETAGHEIPRPIQRMTYADAMARFGTDKPDLRFGLELTEMTEYFADTPFRVFRAPYVGAVVMPGGADQPRRQLDAWQDWAKQRGAKGLAYVLVGQDGTLSGPVAKNLSDGERDGLVKAVGAAPGDCVFFAAGDRSSSRALLGAARLEIGERCGLIDHDAWSFVWVVDAPMFESIDDTDDVAVGSGRWTAVHHPFTSPNADWVDNFESDPANALAWAYDIVCNGNEIGGGSIRIHRSDVQKRVFELLGISEEQAQDKFGFLLEAFKYGPPPHGGIAFGWDRICMLLAGADSLRDVIAFPKSRGTCDLTGKTPAPLELEV",
    "response#3": "MRMRSEVNRIARDVLHGRDFVEVETPTMTRSTPEGARDFLIPARLQPGNWYALPQSPQLFKQLLMVGGLERYFQIARCYRDEDFRADRQPEFTQLDIEMSFVDQDDVIELGEEIISALWRETAGHEIPRPIQRMTYADAMARFGTDKPDLRFGLELTEMTEYFADTPFRVFRAPYVGAVVMPGGADQPRRQLDAWQDWAKQRGAKGLAYVLVGQDGTLSGPVAKNLSDGERDGLVKAVGAAPGDCVFFAAGDRSSSRALLGAARLEIGERCGLIDHDAWSFVWVVDAPMFESIDDTDDVAVGSGRWTAVHHPFTSPNADWVDNFESDPANALAWAYDIVCNGNEIGGGSIRIHRSDVQKRVFELLGISEEQAQDKFGFLLEAFKYGPPPHGGIAFGWDRICMLLAGADSLRDVIAFPFTPRAEPAADPRGRVAALHVPGGAGLTRKQIDDYTQFVGRYGAKGLAYIKVNDPAQGREGLQSPILKFLTDEAVDGILRRTEARAGDLIFFGADKAKVVNDAR"
  },
  {
    "reference": "MTTLACTPATTLVLGGARSGKSAFAERLARDSKLERVYLATASAGDDEMRQRIAHHRAQRGDGWRTVEEPLALVEALERETASGRAVVVDCLTLWLSNLMHADRDIEAETLRLAACLRQSPGPVFLVSNEVGLGLVPDTPLGRCFRDAQGRLNQAIAAVIPNVVFVAAGLPIWLKRFNQEEPTA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Catalyzes ATP-dependent phosphorylation of adenosylcobinamide and addition of GMP to adenosylcobinamide phosphate.2. The GTP binding site should be stable and able to withstand changes in environmental conditions.3. The protein should have Helical domains to ensure proper insertion into the membrane.4. A protein that can modulate the overall flux of metabolites through the adenosylcobalamin from cob(II)yrinate a,c-diamide: step 6/7, adenosylcobalamin biosynthesis, Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed.5. A protein that contributes to cobalamin biosynthetic process, phosphorylation by functioning in membrane with GTP binding, ATP binding, adenosylcobinamide kinase (ATP-specific) activity, cobinamide phosphate guanylyltransferase activity, adenosylcobinamide kinase (GTP-specific) activity.6. A key design requirement for the protein is the optimization of the GMP-histidine intermediate to achieve high catalytic efficiency for adenosylcob(III)inamide phosphate + GTP + H(+) =   adenosylcob(III)inamide-GDP + diphosphate. The designed protein sequence is ",
    "response#1": "MAYSTLRRLARAELIAGIGSGVXGVXDSRALNXGXIFLLVKGALFNGXQFXEQVXEXGAXAAXSEXGMVXPNXDXAXEXGVXIVDSXLXAXAMVSAAXHGXPSXQLXVVXGXQXSXXMMMAXDXXXDXASXSXXVXSXDWXTAXGQXXGXVXDXITTXTXAXAXGXWXWXGXVXAXRXXYXXAAKAXAXADXXXSXXX",
    "response#2": "MTALTGAAALEAFGLPAXALXGXAXSAXXEDXPQXTXEXPLXRXGXSXXLXAXAXAXGXEXSXAXXXDXQXQAXAXAAXGXAIAXPXSXPXQTXAAXSXXXDXXXXXGXNXPXXXVXNXNNXSXXPXCAXXXIPXXXP",
    "response#3": "MIRPATIEEIRAVGPGATLVGRDESGAVPPSTAARPARPAGLADLAARLGIAPVEPADPAEAPEPAAPAPAPLAPAPGSAPADSVEAPGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAAADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASGPAPADGPEASG"
  },
  {
    "reference": "MSENQIKGILGTKLGMTQVFDEDNRVVPVTVVEAGPCVVTQIRTEEKDGYSAIQIAYGDIDPRKVNKPQSGHFAKAGVTPRRYVTEIRMDDVLSDYEVGQDVTVELFGDVKFVDVTGTTRGHGFAGAMKRHGFAGQGAAHGNQAAHRRVGGIGACATPGRVFKGTRMAGRMGNNRVTTQNLKLFKVDADSNLLLIKGAVPGIRGGLVTVKTAVKGGAHA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be optimized for structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "MNIAAEPRTEFGKGGARRTRRAGKVPAVLYGHGKPPRHIALPAHDLLHAFKTDAGTNVLLTLELAEGTELALAKDVQRHPIKGSFEHIDL",
    "response#2": "MSKVVADIPVQVIGEPHPDTLVDQQVTTIAVKADATRLPGPIEVDITGLEPGTSILARQLVLPAGSELDADPDLVVVQCLAK",
    "response#3": "MDPNT"
  },
  {
    "reference": "MSRIAKIYTDKDVTLDIIKGKKIAVLGYGSQGRAWALNLRDSGLNVTVGLERQGKSWDQATADGFKPEKTEDAVKKADVVIFLVPDMAQRLVYRERVQPYLREGMDLVFAHGFNIHYRLIEPPKNVDVYMVAPKGPGPIVRDFYTKGGGVPVLVAVHQNYSGKAMEKALAIAKGLGGTRAGAIETTFKEETETDLIGEQTILVGGVMELMKSAFETLVEMGYQPEVAYFETINELKMIVDLIYDKGFMGMLRAVSDTAKYGGFTVGKHVINEETRRRIREAAEKVRSGKFAEEWIEEYGRGSPTLQKGMEDMDKSMEEQTGRRLKEIIERGKPKA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should exhibit Basic and acidic residues compositional bias.2. A key design requirement for the protein is the optimization of the active site to achieve high catalytic efficiency for (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-   ethyl-2-hydroxy-3-oxobutanoate + H(+) + NADPH.3. For general function, the protein need meet that In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB); In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.4. The protein should have a high affinity for NADP(+).5. The designed protein must have at least one KARI N-terminal Rossmann, KARI C-terminal knotted domain.6. The protein requires Mg(2+) to activate a critical catalytic site.7. The protein design must be able to perform magnesium ion binding, isomerase activity in order to contribute to isoleucine biosynthetic process, valine biosynthetic process.8. Ideal protein should have a binding affinity for key enzymes in the L-valine from pyruvate: step 2/4, L-valine biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MKIYYDDDANLELIKKLNVTIIGYGSQGHAHANNLKDSGVDVTVGLREGSSSWSKASEAGLKVMPVDQSVLNADLVMILAPDEFQKGIFEKSIKPNLKSNAILAFAHGFNIHFKKIVPSETNTVIMIAPKGPGHTVRSTYLNGGGVPSLIAIFNDAMQEGEHSAKDIALSYAKANGGTRAGVLETNFREETETDLFGEQAVLCGGAVELVKMGFETLTEAGYAPEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGTEVINEQSRVAMRNALKRIQTGEYAKMFIQEGKTNYPSMTARRRLNAVHPIETVGGQLRDMMPWPSYYDHGILMVAILKAGIELSFETMIQSGIMEESAYYESLHELPLIANTIARKKLYEMNFREETETDLFGEQAVLCGGASELVKAGFDTLVEAGYAPEIAYFECLHELKLIVDLMYEGGLARMRYSISDTAEYGDYSTGRRIITDETRKEMKKVLAEIQDGTFARNWILENQANRPGFTSRRRLESEHGIEVVGEQLRGMMSHLSVYENN",
    "response#2": "MRVYYDADADQRRLAGRTFAVIGYGSQGHAHALNLRDSGARVIVGLRPGGGSWERARTAGLDVRPVAEAAAASDVIMILVPDQDGRTIYEAGVAGALRPGKTLMFAHGFNIHYGEITPPPGVDVSMVAPKSPGHLVRSEYQAGRGVPGLVAVHQDSSGQSLPNALAYAAGIGCTRAGVIEDFKDETESDLIGEQLVLVGGLIELIKKGFEVLVELGYPPELAYFEACNEAKLIMDLIYERGFTGMLKAVSDTAKYGGLTVGPKVIDEHVKENMRKYAERVRSGEFAKEWISKADKADEVLKELMKEIEEHEIEKVGRFIRREHHVE",
    "response#3": "MRVYYDADADQRRLAGRTFAVIGYGSQGHAHALNLRDSGARVIVGLRPGGGSWERARTAGLDVRPVAEAAAASDVIMILVPDQDGRTIYEAGVAGALRPGKTLMFAHGFNIHYGEITPPPGVDVSMVAPKSPGHLVRSEYQAGRGVPGLVAVHQDSSGQSLPNALAYAAGIGCTRAGVIEDYDGKISEQEYFDNGVLMIAMVKAGVELAFDAMVASGIYEESAYYESLHELPLIANTIARKRLYEMNVVISDTAEYGNYLFSNVATPILAKEIIPNLQKGDLGEPTPAVEVDNITLRDVNDAIRNHPVELIGQELRGYMTDLFSG"
  },
  {
    "reference": "MGQKVHPLGFRVGLNGNYKSKWFVKPKDYSLLLFQDYLIRKNIFNYFNILNNSKNKKNLLDSINITDIKINRKFDQVYIDIYVASMNKVIFNTSGSNSDPLIKIQSNKIVDQLQRVLLKQVSSFNLGNKKVNNSSKIIVNIIESPDINTNAVFIAKCLIDDLENRVPFRRALKTILDTTQKQKNLAGVKIQISGRLNGIEMARSEWVREGRIPLQTLTANIGYYNDVAETKYGLLGVKVWVYKNKS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be involved in translation by localizing to ribonucleoprotein complex and exhibiting structural constituent of ribosome, rRNA binding. The designed protein sequence is ",
    "response#1": "IPPTTALVMKEAGVEKGSGTPNTQAVGNLPLEAVIRIAKMKMESMLSYDLKTAAKEVMGTCVSVGVTL",
    "response#2": "IPPTTALVMKEAGVEKGSGTPNTQAVGNLPLEAVIRIAKMKMESMLSYDLKTAAKEVMGTCVSVGVTV",
    "response#3": "EKRRSFRSIFSQIVKDIPLVMKKGVEGIRICCSGRSKGAEIARTECGKYGKTSRNVFNQKIDYAPAEVSTRYGILGVKVWC"
  },
  {
    "reference": "MAQFSPKPEWLKVRAPGGDTYHHLKETFRKLDLHTVCEEARCPNVGECWREGTATVMLLGDVCTRGCRFCAVTTGDPRGAVDVREPEHVARAIARLSLQYVVMTMVNRDDLLDGGAEHVARTVSRLHALRPDLLIETLVGDFQGHMSAVDMVVDAGPDVFAHNVEVVRRITRVIRDVRSSYDQSLAVLRRAKERQRRLAADAAEAGAPAPRRLTKSSIMVGIGETDDEVLEALRDLREAGVDIVTIGQYLRPSSKHAPVQRFVEPETFAAFERAALEMGFLYAASAPLVRSSYKAAEVFVRSLMDRGGAALPASPGAAAVEALLEERLAVARREAARLTAELDPDEPRPPVAPAPASASPARLVPAASLIRR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be able to compete with endogenous proteins or inhibitors for [4Fe-4S] cluster binding.2. The designed protein must have at least one Radical SAM core domain.3. The protein have enhanced [[Fe-S] cluster scaffold protein carrying a second [4Fe-   4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2   oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-   N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S]   cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-   methionine + 2 reduced [2Fe-2S]-[ferredoxin].4. A protein that contributes to protein lipoylation by functioning in cytoplasm with 4 iron, 4 sulfur cluster binding, lipoate synthase activity, metal ion binding.5. The protein requires [4Fe-4S] cluster to activate a critical catalytic site.6. For general function, the protein need meet that Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. The designed protein sequence is ",
    "response#1": "MIMGDTCTRACAFCNVKTGLPNGLDPHEPAHIADAVQKLGLSHVVITSVDRDDLADGGAEHFAQVIRAIREASPKTTIEVLTPDFLRKDGALEIVVAARPDVFNHNLETVPAKYLSVRPGARYFHSIRLLQRVKELDPTIFTKSGIMVGLGETRNEVLQLMDDGRIACMVNGAGLAMTATIMLMGDTCTRGCRFCSVKTARAPPPLDVNEPVNTAKAISSWGLDYIVLTSVDRDDLPDGGSKHIAETVREIKARNSNIFVECLVPDFRGDLECVKTIASCGLDVYAHNIETVEKLTPFVRDRRAHYRQTLQVLREAKNFNPNLITKSSIMLGLGETDAEVEQTMQDLREVGVECLTLGQYMQPTKRHLKVIEYVTPEKFKHWEGHPDI",
    "response#2": "MIMGDTCTRACAFCNVKTGLPNGLDPHEPAHIADAVQKLGLSHVVITSVDRDDLADGGAEHFAQVIRAIREASPKTTIEVLTPDFLRKDGALEIVVAARPDVFNHNLETVPAKYLSVRPGARYFHSIRLLQRVKELDPTIFTKSGIMVGLGETRNEVLQLMDDRYANAWQFQKLRHDSVLEGIVPDTLLLLEHDPVITMGRGGLPEHIYVTGEILAKKGIELFWVERGGMATYHGPGQLVAYPILHLRDRDLHAYMERLLDVLEATVEHFGVKAERSVHGPGLWVNGAKVASIGMAVRKWVSYHGIALNVSLDLSPFSLISPCGNPTERMTSLERECGKKIDLSDVQKVFVDNWLWTKHTMPARWTEGHPSWLVRHLPSEKEVRPVEHLVSGLGLHTVCQEAHCPNRHECFARGTSTFLVMGDICTRACRYCAVTKGHPKPLDPEEPSRVAEAVARLGLSTAVITSVTRDDVPDGGAGHFAAIVRAIREKSPQTHIELLVPDFQGKQESIERVCAASPDMFNHNIEAVRRIFAQVRPRADYDLSLRVLQKAASLGLRVKSGLMLGLGETWEEIAGTLRDLYDHGCRYLTLGQYLAPSSHHVPMRRYLAPDEFVHWKNVAMDMGFLDVASGPLVRSSYRASAMLRRNAALRHA",
    "response#3": "MIMGDTCTRACAFCNVKTGLPNGLDPHEPAHIADAVQKLGLSHVVITSVDRDDLADGGAEHFAQVIRAIREASPKTTIEVLTPDFLRKDGALEIVVAARPDVFNHNLETVPAKYLSVRPGARYFHSIRLLQRVKELDPTIFTKSGIMVGLGETRNEVLQLMDDGNIARNAEVPIEKKPSWLKNTAKMGPQFTELKSMARGRGLHTVCEEAGCPNIYECWEDREATFLIGGDTCTRRCDFCDIATGKPGMVDVAEPRKVAESVRDLGLRYATVTSVARDDLADGGAWLNAETIRAIHAMNPSTGVEILIPDFKGQPDAVQQVIDAQPEVFAHNLETVPRIFKQIRPAFAYERSLDVLTQGKEHGMIVKSNLILGMGETDDEVYEALCDLHDAGCDIITLTQYLRPGPTFHPIDRWVKPETFVELSKAAEEIGFLGVMAGPMVRSSYRAGKLWAREPGACPVCGSSPVTSHFGGGTATFMILGDICTRRCPFCDVAHGRPEAPDPLESVHLARTVASMKLRFVVITSVDRDDLRDGGARHFAEVISALREHCPELHVEILVPDFRGRVANALAAFRETPPDVFNHNLETVPRLYLQARPGADYHHSLQLLAAFKAQHPQIPTKSGLMLGLGEEIDEIRGVMRDLRQAGCELLTIGQYLAPSRHHLPVARFVPPEEFQQLQRDGMAMGFAVVEGRRGFVEVTERLPKPQWLRVRSPLSPEVDQLKKILRDAALHTVCEEASCPNLGECFGGGTATFMILGDICTRRCPFCDVAHGRPEAPDPLESVHLARTVASMKLRFVVITSVDRDDLRDGGARHFAEVISALREHCPELHVEILVPDFRGRVANALAAFRETPPDVFNHNLETVPRLYLQARPGADYHHSLQLLAAFKAQHPQIPTKSGLMLGLGEEIDEIRGVMRDLRQAGCELLTIGQYLAPSRHHLPVARFVPPEEFQQLQRDGMAMGFRHVASGPLVRSSYHAERSFH"
  },
  {
    "reference": "MKSLAELGLRAQGGREAQITGLAVDSRDVKPGFLFAALPGARVHGGEFIQYALRMKAGAILTDATGAELAKSELAESDAALIVTEDPREALAQTASLWFGAHPATVVAVTGTNGKTSISSFCRQIWEELGIAGVNLGTTGVEGVWTHPLAHTTPEPITLHRVMAEAAQNGVTHVAMEASSHGLDQRRLDGVILKAAGFSNFTQDHLDYHKTFEAYFDAKMGLFRRVLPQDGTAVINLDDPKGPQVAQISNERGQSVMGVGRHADARLKLTGQRFDATGQDFLFQWDGKSRQTRLNLIGGFQAENVLLAAGLVIAAGADADAVFDTLPHLKTVRGRMELAATRESGAAVFVDYAHTPDAIETALKAMRPHVMGRIIVIVGAGGDRDTSKRPLMGKAAAENADVVIITDDNPRSEDPATIRRAVMDGALDIGDPAVISEVGDRAEAILRGVDMLTAGDALLIAGKGHETGQTVGDTVLPFDDVEQASVAVAALEGRI",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have a high affinity for ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate.2. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..3. The protein design must feature the Meso-diaminopimelate recognition motif motif.4. We require a protein that can specifically bind to the metabolites produced in the peptidoglycan biosynthesis pathway.5. The presence of Mur ligase central, Mur ligase N-terminal catalytic, Mur ligase C-terminal domains is critical for the interaction of the designed protein with its intended targets.6. The protein should have a low Km value for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.7. The designed protein must possess magnesium ion binding, ATP binding to support cell cycle, cell wall organization, regulation of cell shape, cell division under certain conditions. The designed protein sequence is ",
    "response#1": "MKLSELLKRVQCDLVGQDVVEGIAHDSRRVKPGDLFVALRGTKEDGHRFVPDALKAGASALLLEEPLPWAVPVPWVVVPDAREALASVAARFYDNPSSRLKLIGVTGTNGKTTTTHIIEHILNDQGYKVGLIGTIETMIDKKTVVAQNTTPESYEVQRYFAEMVSAGCEYCVMEVSSQALMLHRVDCCEFDVGIFTNLEPDHIGENEHKDFEDYQAAKRRLFEQCRVAIVNVDDQYGDRVVADTNAELLTYSLGKNTDYRATDLKMEKNAVGIELALAGRMDRTVVEIPIPGKFNVDNALTAIAVCRHFGLTEAQIRQGLESVQVRGRFERVQAPEGITAIVDYAHTPDALDNVLKTIDTFRAGETQVITVVGCGGNRDKTKRPVMARVAAQWSDRVFLTSDNPRDEEPEAIINDMRAGLDPIDAKKCIAITDRREAIRMAAQLAGEGDIVLVAGKGHEDYQIIGHEKIHFDDREVARKVIAERMR",
    "response#2": "MKLRDLLQPLQWAQVVGDLELEIEHICYDSRKSAKNALFIAVPGTQVDGHLFIDKAIELGAIAVVCERQLPPGVEGFIYVSDARRAMALAAANFYDYPSEKLKLIGVTGTNGKTTTTHIIEHILNDQGIKTGLIGTIENIIGDRVIPARNTTPDSLTLQELFAQMVEAGCQACVIEVSSHALAQRRVAGTTFAVGVFTNLTQDHLDFHKTMEEYFQAKAKLFTMLGNDRNVAVVNIDDEYGQRIVAETEASVLTYAVGNGADLRAVDLQLSSNGTDFTLRVGEHSFPVRLPLAGRFNVYNALAAITASLLLGVDLEVVVDAMSQLQAVPGRFQYLISDNKITAIVDYAHTPDALKNVLETINSIRTKNEELITVFGCGGNRDKTKRPKMGHIASALSSKVIITSDNPRNEAPESIMEDIEKGIEPQNFKKVLSIADRKQAIKTACQMANPNDIILIAGKGHETYQIMGTQKIHFDDREVAREAIAEKQ",
    "response#3": "MKLSEILKHYQVTHPNGDAEKITSLECDSRKIKRGSLFVAVRGYNVDGHKFIPLVTLAGAAAIVCEELPERLESTVTYIKVPDSAVALGLLASQQFDQPSSKLKMIGVTGTNGKTTTTHIIEHILNDQGFTVGLIGTIEAIISDDTIKADNTTPESYVVQKYFAQMKDAGCTHVVMEVSSQGLKLHRVHGFTFDYGIFTNIEPDHIGPNEHKDFEDYLHCKGLLFKQCKIGICNSDEDHFHQVIEGHTCSLETYGFADDADLRATDPHLVSNPGKLGITGTNGKTTIATLLFKLFRELGYKVGLISTVENQINDQIIPATHTTPNPIALNQLLRQMVTEGCQFAFMEVSSHAVVQHRIEGVSFVGGVFSNITHDHLDFHKTFDNYIKAKRAFFDALPKTAFALTNGDDKNGLVMLQNTKAGRKTYALKQLADFKGRIIENKFSGLNLEVDDVDVFFKLVGSFNAYNLLAVYGAAMLLGQDKDEVLKALASAKVKGRLEPVSSEGIDVQGFVDYAHTPEAVHRVVSEIRNLVAADRHLSVVIGCGGDRDASKRALMGQAAAASSDLVVVTDDNPRSEDPAAIRAAVISGAATQSAEVVEQGDRAQAIAAAVQRTHQAGGGVVLVLGKGHETYQETAGVRHPFDEREVVLQALEERV"
  },
  {
    "reference": "MKLHNLRPAKGGEVKARKRVGRGYGSGLGHNAGRGRDGQNSRSGGGVRPGFEGGQMPLFRRLPKRGFKNHFAKQYAEINIRDLNCFEDGEEITPEILANAGFFKPAKAKDGVKILGDGEITKKLTIKANKFTKSAEEKITKAGGKVEVI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC",
    "response#2": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTCA",
    "response#3": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC"
  },
  {
    "reference": "MTQPLHVVILAAGQGSRMKSALPKVLHKVAGRPMLHHVIGTARSLGAAGIHGVIGHGADQVREVTPADDIQWALQTEQLGTGHAVAQALPQLPDDARVLILYGDVPLTRQDTLAHLADQVSDQSIGLLTVRLDNPDGYGRILRDADNKVLAIVEQKDASAEQLAVNEVNTGILAVSARHLKDWLPQLSNSNAQGEYYLTDVISMAVERGIEVKVAHPETEYEVQGVNNRLQLAELERWYQLNEARRLMTEGATLADPARVDVRGPLTIGQDITIDINVVFEGEVTLGNRVSIGPNCLIRNASIANDAVIEANSVIDGAQVGEGAHIGPFARLRPGTELARNTKVGNFVETKKAIVGEGSKINHLSYVGDAELGRDVNVGAGTITCNYDGVNKFQTRIGNGVFVGSNSSLVAPVTIAEGATIGAGSTITRDVEPEELAVARGKQRNIQNWQRPRKQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein with the reaction acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate, the Proton acceptor must be optimized to ensure efficient catalysis.2. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).3. The designed protein should have a high specificity for acetyl-CoA binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "VIMAAGKGTRMRSAQPKVLHRLAGRPLLGHVLATAAGLAARRLIVVTGHGADAVEAAMRADFPGLPLAFVRQEPQLGTGHAVQQALPVLGEAGTTLILNGDVPLIGAATARALVQACGGERLALLTTQLPDPTGYGRITRSADGTVARIVEEKDADAGERRITAVNAGVYVFRADVLHRALTQVTTDNAQGEYYLTDALALAREDGTVVAPLVAPDALQTEGANDRAQLARLRAELNRRVVEAHMRAGVRLADPARFDLRGTLACESDVEIDVNCVFEGAVSLGAGVRVGANCVIANARIETGAVIHPFTHIDGEKAGVTVGEGSRIGPFARLRPGARLGAEVHIGNFVEVKNSTLAAGAKANHLAYLGDATVGARVNYGAGSITANYDGANKHRTVIGDDVHVGSNCVLVAPVTIGVGGTVGGGSTVTKSTEPGALTVARARQRVIEGWTQRRRPGTPAAEAAERALEADGPGAASAPHEPPEGDAGAPAHEQQ",
    "response#2": "IVLAAGQGTRMNSDLPKVLHQIAGAPMLGHAIHAGMALEPARIVVVAGHGAEAVTKAAEDINPFAQVVLQEEQLGTGHAVDQARDILGGFDGDVVVLYGDTPFIQPETLQAMAEARAKNDLIILDSDDPTGYGRIVRNNNGEVLRIVEQKDANNNELQIREINTGIYCVDNQVLKDIIPQLNNNNQQGEYYLTDVIELAVKNNANVSAIHAPEANEILGINDRLQLAQAERILRQNIINKHMLQGVMLRDPSRFDLRGELTCGQDVDIDINVIIEGKVSLGYGVKIGAGCILTNCTIADNTEIKANSIIEGANIGENASIGPFARIRPDTEIANEVHIGNFVEVKKSSLGEGTKCGHLSYIGDSQVGSGVNVGAGVITCNYDGANKHQTIIGNDVFVGSDCQIVAPVTIGHGATTGAGTTVVNDVPDNALCISRTKQRNIANWKRPKK",
    "response#3": "IVLAAGQGTRMNSDLPKVLHQIAGAPMLGHAIHAGMALEPARIVVVAGHGAEAVTKAAEDINPFAQVVLQEEQLGTGHAVDQARDILGGFDGDVVVLYGDTPFIQPETLQAMAEARAKNDLISLELEDPTGYGRIIRDDSGNVLAIVEQQDATPEQLQISEVNTGILAAPTKHLRQWLGQLSNDNAQGEYYLTDIVAMAVAEGVPIVTAQPTNEWETLGVNSKVQLAELERAYQRDVAERLLEQGVRLADPARFDLRGTLACESDVEIDVNCVFEGAVSLGAGVRVGANCVIANARIETGAVIHPFTHIDGEKAGVTVGEGSRIGPFARLRPGARLGAEVHIGNFVEVKNSTLAAGAKANHLAYLGDATVGARVNYGAGSITANYDGANKHRTVIGDDVHVGSNCVLVAPVTIGVGGTVGGGSTVTKSTEPGALTVARARQRNIEGWKRPVKIKK"
  },
  {
    "reference": "MNQSLFQFSESFAREMDAKDSLAHFRSRFYFPKVNGKKAIYFCGNSLGLQPKSVKDYLEKELNNWADRAVDGHFHGEDAWYHVRKKSKPALAHIVGAQEHEVVAMNNLSVNLHLLMVSFYRPTKERYKIIVEAGAFPSDQYMLETQIKFHGLDPKEVLVELSPRTGEFTLRTEDIVAEIEKHGDSLALVNMAGLQYYSGQLFDIKAIVAAAHSVGALAGFDLAHAAGNAVLKLHEWNVDFATWCSYKYLNSGPGNVSGIFVHERFAERPDLPRFAGWWGHDEGERFKMEKGFVPMYGADGWQLANSNVLALAVHQASLDIFAEAGMENLRAKSEKLTGYLEFLIHEISGESGVLEIITPKSPAERGCQLSLFIHCGGKEVFDEWYNQGVVGDWRNPNVIRLAPTPLYNSFLDVFRFAKILEQSLKKFA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be able to regulate tryptophan catabolic process.2. A protein that is capable of catalyzing reactions within the quinolinate from L-kynurenine: step 2/3 pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "GEHNLRLEDILAKIAQVGDALALVLIGGVNYYTGQVFDMKAITQAGHQVGAKVGWDLAHAAGNVKLHLHDWNVDFAAWCSYKYX",
    "response#2": "GEHNLRLEDILAKIAQVGDALALVLIGGVNYYTGQVFDMKAITQAGHQVGAKVGWDLAHAAGNVKLHLHDWNVDFAAWCSYKYR",
    "response#3": "GEHNLRLEDILAKIAQVGDALALVLIGGVNYYTGQVFDMKAITQAGHQVGAKVGWDLAHAAGNVKLHLHDWNVDFAAWCSYKYH"
  },
  {
    "reference": "MEASPFLSACILLPLIRGHSLFTCEPITVPRCMKMAYNMTFFPNLMGHYDQGIAAVEMEHFLPLANLECSPNIEMFLCQAFVPTCTEQIDVVSPCRKLCEKILSDCQKLMDTFGIRWPEELECHRLPYCDGAVPVTSDPHTELFGPQKKTDQVPRDIGFWCPKHLRTSGDQGYRFLGIDQCAPPCPNMYFKSDELDFAKSFIGIVSIFCLCATLFTFLTFLIDVRRFRYPERPIIYYSVCYSIVSLMYFVGFLLGNSTACNKADEKLELGNTVVLGSKNKACSVVFMFLYFFTMAGTVWWVILTITWFLAAGRKWSCEAIEQKAVWFHAVAWGMPGFLTVMLLAMNKVEGDNISGVCFVGLYDLDASRYFVLLPLCLCVFVGLSLLLAGIISLNHVRQVIQHDGRNQEKLKKFMIRIGVFSGLYLVPLVTLLGCYVYELVNRVTWEMTWFSDHCHQYRIPCPYQANPKARPELALFMIKYLMTLIVGISAVFWVGSKKTCTEWAGFCKRNRKRDPISESRRVLQESCEFFLKHNSKVRHKKKHGKPGSHKLKVISKSMGTSTGVTTDHGTSAVAIADHDYLGQETSAEVQTSPEASLKEGRADRANTPRAKGPDCGEPASTAASNSKFSGDQIDRKSRAGSVKEKSSVSEGAPSEGRVSPKGVPGTGLMECTLQIPSSPEPNSLKGSTSLLVRSASGTRKEQGAGGHSDT",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should exhibit Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "VIFTARFEGEPRSVTNDIHKKILLVKKLAYALGDRNSSTVSLRNITKGSIVVEWTNTSLPQHPCPKEQLTVMSRTLANADGRPSQTFRYSMEPEFRPLDVMVKGRASCRTYSFIPPGEIDIPEPPAVTPALGTGRQSTDDVYLHTVIC",
    "response#2": "VIFTARFEGEPRSVTNDIHKKILLVKKLAYALGDRNSSTVSLRNITKGSIVVEWTNTSLPQHPCPKEQLTVMSRTLANADGRPSQTFRYSMEPEFRPLDVMVKGRASCRTYSFIPPGEIDIPEPPAVTPALGTGRQSTDDVYLHTVIC",
    "response#3": "KTPVIFTARFEGEPRSVTNDIHKKILLVKKLAYALGDRNSSTVSLRNITKGSIVVEWTNTSLPQHPCPKEQLTVMSRTLANADGRPSQTFRYSMEPEFRPLDVMVKGRAC"
  },
  {
    "reference": "MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTSRRAAEHRDTTDVPEMPHSKGQGESDPLDHEPAVSPLLPRKERGPPEGGLNEDERLLPKDKKTNLFSALIKKKKKTAPTPPKRSSSFREMDGQPERRGAGEEEGRDISNGALAFTPLDTADPAKSPKPSNGAGVPNGALRESGGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSASCVPHGAKDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein contains novel SH3 domains that confer a unique function or activity.2. The protein's architecture should predominantly feature strand-strand-turn-helix-helix-strand-strand-strand-strand-strand-helix-strand-helix-strand-helix.3. For general function, the protein need meet that Positively regulates chemokine-mediated T-cell migration, polarization, and homing to lymph nodes and immune-challenged tissues, potentially via activation of NEDD9/HEF1 and RAP1 ; Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes; ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death; Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI14. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-   [protein].5. The designed protein should have SH3 domain binding to facilitate protein phosphorylation.6. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MGFGSDLKNSQEAVLKLQDWELRLLETVKKFMALRIKSDKEYAYTLQNLCNQVDKDSTVQVNYVSNVSKSWLLMIQQTEQLSRIMKTHAEDLNSGPLHRLTMMIKDKQQVKKSYVGIHQQIEAEMIKVTKTELEKLKSSYRQLIKEMNSAKEKYKEALAKGKETEKAKERCDKATMKLHMLHNQYVLALKGAQLHQSQYYDTTLPLLLDSVQKMQEEMIKALKGIFDEYSEITSLVTEEIVNVHKEIQMSVDQIDPSTWYHGAIPRIEAQELLKQQGDFLVRESHGKPGEYVLSVYSDGQRRHFIIQFVDNLYRFEGTGFSNIPQLIDHHFNTKQVITKKSGVVLLNPEPWYFGKIGRKDAERQLLCHGNPRGTFLIRESETTKGAYSLSIRDWDEVKGDHIKHYKIRKLDNGGYYITTRAQFEAVQQLVQHKPLAEHDWYHGAIPRIEAQELLKQQGDFLVRESHGKPGEYVLSVYSDGQRRHFIIQFVDNLYRFEGTGFSNIPQLIDHHFNTKQVLEYVRALFDFNSDRVRAEMPNQLRKQLEAIIATDPLNPLTAEDKELLWHFRYESLKHPKAYPKLFSSVKWGQQEIVAKTYQLLARREVWDQSALDVGLTMQLLDCNFSDENVRAIAVQKLESLEDDDVLHYLLQLVQAVKFEPYHDSALARFLLKRGLRNKRIGHFLFWFLRSEIAQSRHYQQRFAVILEAYLRGCGTAMLHDFPKWFHEGLSRHQAENLLMGKEVGFFIIRASQSSPGDFSISVRHEDDVQHFKVMRDNKGNYFLWTEKFPSLNKLVDYYRTNSI",
    "response#2": "MELENYKQPVVLREDNCRRRRRMKPRSAAASLSSMELIPIEFVLPTSQRKCKSPETALLHVAGHGNVEQMKAQVWLRALETSVAADFYHRLGPHHFLLLYQKKGQWYEIYDKYQVVQTLDCLRYWKATHRSPGQIHLVQRHPPSEESQAFQRQLTALIGYDVTDVSNVHDDELEFTRRGLVTPRMAEVASRWFHEGLSRHQAENLLMGKEVGFFIIRASQSSPGDFSISVRHEDDVQHFKVMRDNKGNYFLWTEKFPSLNKLVDYYRTNSISRQKQIFLRDRHGWLYEGLSREKAEELLLLPGNPGGAFLIRESQTRRGSYSLSVRLSRPASWDRIRHYRIHCLDNGWLYISPRLTFPSLQALVDHYSELADGDELSFQEGAIIYVIKHDWYHGAIPRIEAQELLKQQGDFLVRESHGKPGEYVLSVYSDGQRRHFIIQFVDNLYRFEGTGFSNIPQLIDHHFNTKQNDPHLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSCDRKFRVKIRGIDIPVLPRNTDLTVFVEANIQHGQQVLCQRRTSPKPFTEEVLWNVWLEFSIKIKDLPKGALLNLQIYCGKAPALSSKASAESPSSESKGKVQLLYYVNLLLIDHRFLLRRGEYVLHMWQISGKGEDQGSFNADKLTSATNPDKENSMSISILLLSGEWWRAQSLPSVHVAKVSHGWLYEGLSREKAEELLLLPGNPGGAFLIRESQTRRGSYSLSVRLSRPASWDRIRHYRIHCLDNGWLYISPRLTFPSLQALVDHYSELADDICC",
    "response#3": "MELENYKQPVVLREDNCRRRRRMKPRSAAASLSSMELIPIEFVLPTSQRKCKSPETALLHVAGHGNVEQMKAQVWLRALETSVAADFYHRLGPHHFLLLYQKKGQWYEIYDKYQVVQTLDCLRYWKATHRSPGQIHLVQRHPPSEESQAFQRQLTALIGYDVTDVSNVHDDELEFTRRGLVTPRMAEVASRSSEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYWFHEGLSRHQAENLLMGKEVGFFIIRASQSSPGDFSISVRHEDDVQHFKVMRDNKGNYFLWTEKFPSLNKLVDYYRTNSISRQKQIFLRDRISLDKKLGMGCFGDVWIGTWNGTTKVAVKTLKPGTMSPEAFLEEAQIMKRLRHDKLVQLYAVVSEEPIYIVTEFMSQGSLLDFLKDGDGRFLKLPQLVDMAAQIAAGMAYIERMNYIHRDLRAANILVGDNLVCKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGKFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMQCPGNCPPSLHELMVQCWKKEPEERPTFEYLQSFLEDYWLYEGLSREKAEELLLLPGNPGGAFLIRESQTRRGSYSLSVRLSRPASWDRIRHYRIHCLDNGWLYISPRLTFPSLQALVDHYSELADDICCLLKEP"
  },
  {
    "reference": "MIVKTRAVILREIKYRDQSKICSIYTREFGKMSIIIKGARNPKNRLSGLFSAGNVVDLVLYKKSSRDIQLATDGNLVLSPMVPEPDLERFAILYRIIDFVRHTTENDEKNLPLFTLLTGTLEQLYHTNVNFQQLYAWFLLRFVSLLGFQPSLRTCVFSGEELLPAIEALQLTELYFVMNPGGLALPKAAGSSLAKKQLIPVRLAMLLSALAATRLPAGDSIKADPREIEDLWNLLQEYCSLHLEHARGRKNLDIVSQILLK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must be able to regulate DNA repair. The designed protein sequence is ",
    "response#1": "FIHAADLHLDSPFYGISHLPEPIFARIKESTFASVRHMIDAAVREHVDFILLAGDLFDEANRSLKAQLFLKKQFERLRECGISVYVIFGNHDHLGGEWTPIEWPENVHIFSSAVPEEKSFFKEGRRIASIYGFSYQARALMENQAARYRRSTDAPFHIGMLHGTLSGSEGHDPYCPFTHDDLVKSGMDYWALGHIHKRQVLSAEHPAVIYPGNTQARHCG",
    "response#2": "FIHAADLHLDSPFYGISHLPEPIFARIKESTFASVRHMIDAAVREHVDFILLAGDLFDEANRSLKAQLFLKKQFERLRECGISVYVIFGNHDHLGGEWTPIEWPENVHIFSSAVPEEKSFFKEGRRIASIYGFSYQARALMENQAARYRRSTDAPFHIGMLHGTLSGSEGHDPYCPFTHDDLVKSGMDYWALGHIHKRQVLSAEHPAVIYPGNTQARHG",
    "response#3": "QIYPKSFQDTTGSGTGDLRGVIQHLDYLHKLGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMVFNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYYLHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGDGRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFNFHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFGDEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELRNDGRDADELLAILASKSRDNSR"
  },
  {
    "reference": "MKTKFIFVTGGVASSLGKGINASSIGRRLKCRGLKVFMQKFDPYINVDPGNMSPLQHGEVFVTDDGTETDLDLGHYERFIDENLSKYSSVTTGKIYLRVIEKERNRYYDGATVQVIPHITDEIKNAILDAAKFSGADVVVTEIGGTVGDIESLPFLEAIRQFRRDVGYKSTMYVHNTLVPYISASQELKTKPTQHSVKELRSLGIIPDIIILRTEKKLSNGQRDKIALFCDVRKECVIEALDCEVLYDVPIMLAEQNIDQIICDHFGIETAPLDLSEWRSMVEHIKGLKKKVQIALVGKYVTLKDSYLSVYEALKHAGYANGVEVETVPIDSGTITSENVAEKLSRFQGVIVPGGFGERHIRGIVEAIKYARENKIPYFGLCFGMQLALIEFARNALGLEKATSRELDPNAELPIIDLRENGQSMRLGLYSCELLPGTKVREIYGKEIIYERHRHRYEFNKRLSHLFEGTDLVFSGINRENGVLELIELKNHPFFVACQFHPEFLSRPNREHPLFRAFIKAALAEAEK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be optimized for activity in CTP from UDP: step 2/2, Pyrimidine metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The designed protein have CTP synthase N-terminal, Glutamine amidotransferase domains with specific sequence motifs that are crucial for its activity.3. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.4. The L-glutamine, UTP, ATP, Mg(2+), CTP binding site should be located in a region of the protein that is accessible to the ligand.5. The designed protein should be optimized for metal ion binding activity in order to improve 'de novo' CTP biosynthetic process, glutamine metabolic process.6. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile; for glutamine hydrolysis, Nucleophile, active site to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+). The designed protein sequence is ",
    "response#1": "KYVFVTGGVVSGLGKGITAASLGRLLKNRGLKIAAQKLDPYMNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDEDLNKFSNMTSGKVYWNVLTKERNGEYLGSTVQIIPHVTNEIKSFIYAVGRKTNADVVITEIGGTIGDIESQPFLEAIRQVSREVGESNCLFIHVTLVPYLRSSGEHKSKPTQHSVKILQGSGITPHIIVTRSDEPLDQSTKDKIALFCNVKRDCVIENRTVPVLYQAPVMLASEGLDEIVCREMNVRISLVGKYTQLHDAYLSVMESLKHAGWENQSRVSISWIDSECVTPDSVDDLLAGSDGILIPGGFGDRGIEGMIVAARYARENKIPYFGICLGMQIAVIEYARDVMGLVDANSSEFAPNGLHSVIDLMPDQMGNIPKGGTMRLGEYPCAIVPETIMNRAYRGEAEIKERHRHRYEFNNVFREQLVKAGLTISGHSPDGHLVEAVELPEHVHPFYLGVQFHPEFKSRPNRAHPVFREFVAAAQARVKQAEQVKIAIVGKYTGLKDAYKSLQEALVHGGIANNVRVNITWLASEIFEQDHEAILALEDVNAILVPGGFGERGTSGKIAAVQFARERKVPYLGICYGMQMAVIEAARNLAGLKDANSTEIGPTRNPVVGLMTEWVRGNRTELRQEGGDLGGTMRLGAYDAVLSEGSRVAEIYGTRLISERHRHRYEVNPAYRRHLEEHGVLFSGMSPDGILPEVIEIKDHPWFIGVQFHPELKSKPLDPHPLFSSFVQAALEAK",
    "response#2": "MTKYIFVTGGVVSSLGKGIVAASLGRLLKNRGLKITIQKFDPYINVDPGTMSPYQHGEVFVTDDGTETDLDLGHYERFIDINLNKYSNVTTGKIYSQVLQKERHGDYLGATVQVIPHITDAIKEKILRAGTSTDADIVITEIGGTVGDIESLPFMEALRQMKRDVGSENVLYIHTTLIPILHAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEKAITEDMRKKIALFCDVDPEAVIESRDVDILYQIPLNLQKQNMDQIVCDQLGMDITKPDLSDWETLVNRLKNPKYQVTIALVGKYVSLHDAYKSIVESFIHAGAANEVKVNVKWIYSGDVNESNAASLFKDVDGILVAPGFGERGIEGKITATKYAREHGIPFLGICLGMQMAVVEFARDVLGLKDAHSKEINPETPYPVIDLMESQKNVTNKGGTMRLGAWQCSVEKDSRAYSIYGTDSIMERHRHRYELSSDYIEALDKAGMKATGRNAETGLVDIVEIPSHPWFVAVQFHPEYRSTVENPHPLFVDFVRAAVVQAQKTKDAYKSLIEALSHGGIANKVKVNLDWIESEVFEREDPAPFLEHVNGILVPGGFGQRGAEGKIKAVRFARERRVPYFGICFGMQMAVIEAARNLVGIEGANSTEFGETKEPLVGLMTEWLRGNELEKRSKAGDLGGTMRLGAFPAALRPGSRVSKIYGNALEISERHRHRYEVNTAYKDRLEQHGLRFSGMSPDGVLPEIVEYEDHPWFVGVQYHPELKSRPLDPHPLFASFIR",
    "response#3": "MTKYIFVTGGVTSSLGKGIIAASLAKLLQARGFTCTIQKFDPYINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERYLNTFTSQANNVTTGRIYQHVINKEREGAYLGKTVQVIPHITDEIKRRMLLLGEDKQFDIVITELGGTVGDIESLPYIEAVRQLKWEMGTDCLVIHLTLIPYLKAAKELKTKPTQHSVKLLSEQGLSPDVLVCRTEQPLYNDLKRKIALFCNVTPDAVIEAMDADSIYSVPLEMMREKLNIPAKAEPQLSSWRKFMENLKNPKRSVKIAIVGKYFDVGDFELSDSYISVIEAIKHASAKNEVKAEISWINSKDFESDEKKVEILKNFDGIVVPGGFGASGVEGKILAIKFARENNIPFLGLCFGFQLAVVEFARNVCNLKDAHTTEVNPNTPHPVIDLLPWQKELLKESKYGATMRLGGQTVRIKKGTLAYNLYRKEEVVERFRHRYEVNPKYVKILEKNDFVFSGFSKKEENIMQIGELPSHKFFIGTQFHPEFTSRPLKPNPIFDGFVNACIKIAK"
  },
  {
    "reference": "MELKTLVNHLINYKTDDEMNVEITSIEMDSRTAGPGSLFVCIEGYTVDGHNYARQAVENGASAILAEKPLEEIDSVPVVIVNDTKRSLAVLANVFYDHPTTKLHLIGVTGTNGKTTTSHLIEQIFADAGKRTGMIGTIDMKINGVSHKVSNTTPESLFLQKAFKEMNDQKVEAAVMEVSSHALDMGRVRGCDYDIAVFTNLTQDHLDYHKDMETYLRAKGLLFAQLGNTYAGKKEKIAILNRDDKASKEFEKMTAAQILTYGIEQKSDIMAKDIKVTGKGTTFTLVTPFEEKQVSLKLVGKFSVYNVLAAVSATLLSGIPIDSIIRTMENVKGVPGRFETVDEGQCFTVIVDYAHTPDSLENALVTVKEFAVGKVISVVGCGGDRDRTKRPLMAKIAAEYSDIAIFTSDNPRTEDPEAILQDMQAGVEDREYKVIMDRRDAINYAVNLAEENDIILIAGKGHETYQIIGKDVLDFDDRKVAAEAIGAK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be able to bind meso-2,6-diaminoheptanedioate ligand in a variety of conditions2. The designed protein must be optimized for magnesium ion binding.3. The designed protein must have at least one Mur ligase N-terminal catalytic domain.4. The protein should have a low Km value for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "MRLKDLFEKIEVIQIHGDKEVEISGISYDSRDVKTGDLFVAWKGEHYDGHDFINDAVSRGAAAVMSETVVEIPSGVVKYADRNPRRAFAELASRFYGYPGQKLKLVGITGTNGKTTTATFLYEILKLQNIKSGLISTNCIKIQDKKFDTNLTTPDSYEINFYLNEMVSHGVEYCFMEVSSHSIVQDRIKGLNFKIGVFTNISHDHLNYHKTFKNYIEAKKIFFDNLSSKSISLYNSDDKNGRIMIQNTSSKIKSFSIHKNSDYKIKIVENNLEGLVVEIQKNVIHSRISGNHNAYNLLAAYAVAVSLGFSVEDIVSGLKAAPPVPGRLEPVPHPGGVHVYVDYAHTPDALEAVLRTLRGLHGDRVVAVFGCGGERDREKRPMMGAIASRLAREVVLTSDNPRREDPEDIISQIFAGCDPGLARVHREPDRREAIRLALSRVTPGDALLVAGKGHEDYQILKDRTIHFDDREEAREALKLLG",
    "response#2": "MSLNLPEITGLTADSREVGPGMLFAALPGTLTDGAGFIPQAIEKGAAAILLPQGSSAAGGLKLPVLEEADPRRALALMAARFYGRQPETIVAVTGTSGKTSVADFTRQIFTSLGHKAASVGTIGIVKPDGAVYGSLTTPDPVTLHATLAALADEGVTHLAFEASSHGLDQNRLDGVELTAAAFTNLGRDHMDYHPTEEAYLNAKIRLFTELLRAGQPAAINMDGARSADVAAVAKARGLRVLTVGALGETLKRTSLTRDGFQQRIGILSEGRHCEVRLPLVGAYQAENALVAAGLALCAAGVPFAQALVTGTNGKTTSSVLIAAVEHALGHRTAVVNTLGAHVDGHVTRFELALPPPARLQRFLRDRRDDGHGGVVLECSSWSLHLDRPAGVAFDRVLVTRLTRDHLDMHWDMQAYARAKWRLVEALGNSGKPQRHLSLAASFPAPGPVPHDVSCLRFGLVPDGADVWARDVSSSPAGTSFEVVWRGRPAGRLRTNLPGAHNVENLLGVVALLDAVGVPLADALAGLGKAVPVAGRLEPVPHPGGVHVYVDYAHTPDALEAVLRTLRGLHGDRVVAVFGCGGERDREKRPMMGAIASRLAREVVLTSDNPRREDPEDIISQIFAGCDPGLARVHREPDRREAIRLALSRVTPGDALLVAGKGHEPYQEVAGVRHPFSDAAHARAALRARGDA",
    "response#3": "MTLDSRKVSNGDLFIALKGHNVDGRTFIPKAIEQGASLILAEADEGVQAVKIAKEFVNATACVLSVPHLPKLLSQIAGEFYGYPSRQMRLVGITGTNGKTTTATFLYEILKLQNIKSGLISTNCIKIQDKKFDTNLTTPDSYEINFYLNEMVSHGVEYCFMEVSSHSIVQDRIKGLNFKIGVFTNISHDHLNYHKTFKNYIEAKKIFFDNLSSKSISLYNSDDKNGRIMIQNTSSKIKSFSIHKNSDYKIKIVENNLEGLVVEIQKNVIHSRISGNHNAYNLLAAYAVALHLGVSLREAISILPAVSGVPGRFEHFVSPSGVLVIVDYAHTPDALEKVLLAILEFKSQYSKLISVFGCGGDRDPLKRRIMGKIGAMHSDIAIFTSDNPRNENPESIIEEMKTDLSLELSEKVKSILDRREAIIEAVKIAEKGDIILCAGKGHEDYQEIKGVKHHFDDREVLREALKRRR"
  },
  {
    "reference": "MTDPSTAPAPTVAVILLAAGEGLRLGLGEPKAFVHLAGRPILAHALESLAGMRRHPQIVLAVPPSHEAIARELLHGVPGLDVERATIVVGGPSRQASVRIALGHLAASIETVLVHDAARPLAPAVIFDSVAAEVERTGEGVITGLAVSDTIKRLADDDRVVETLDRSQLTAVQTPQGFPRAWLEEGHARATEDHTDDAALVSELGRPVRVIAGDQLASKITTTWELRRTEEMLQHDPASTIRVGTGTDVHAFHPDAELWLGGLHWPGEVGLAGHSDGDVVAHAICDAMLSAVRLGDVGEVFGTSDPRLAGARGDVFLAETLRLVREHGYELVNVAVQVVGERPRIAGRRREVEAHLSAVLGASVSVAGTTSDALGFTGRGEGVAAIATALVRSATSQQTGPLGA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The presence of a divalent metal cation is crucial for the protein to function optimally under specific conditions.2. For general function, the protein need meet that Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D- erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C- methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).3. The protein should be optimized for activity in Isoprenoid biosynthesis, isopentenyl diphosphate biosynthesis via DXP pathway, isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6 pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The designed protein have 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "ILAGDTKVETRVGSGFDVHAFAQDGDAVILGNISIPHTVGLSGHSDADVVLHALSDAIYGALADGDIGKHFPPSDMQWKGADSSIFLEHATKLVGERNGRLVHLDVTIICEQPKIGPHVAAMRSRIAEISGVTMDRVAVKATTSERLGFTGRQEGIAAQAVATIEIGSAGTKAATTDDAAVAEWAGHRVHVFAGEDANMKVTTCKDLAIAEARLLGDLADVRTGQGYDVHAFGPGAHVWLGGLKIAHNHGLIGHSDADVLTHAITDALLGALADGDIGSHFPPSDPQWRGAASSIFLAAAAAKVRSRGGIIAHIDATLVCERPKLGPHRDAIRASIARIAEIPLDRVAIKATTSEGLGFTGREEGIASFAVATVVIGD",
    "response#2": "AMAEALIPRQTRVGQGFDAHRWGPGSSVWLCGVEIPHDQSLIGHSDADAGLHALTDALLGAMGDGDIGDHFPPTDPQWKGAASDLFLIHAVERLHRRGGRLVNVDVTLICERPKVKPHRQAMRERLADLLSLPLDAVSVKATTTEAMGFTGRGEGLAAQAIATVVEARRPGETDDAAVAEWAGHRVHVFAGEDANMKVTTCKDLAIAEARLLGDLADVRTGQGYDVHAFGPGAHVWLGGLKIAHNHGLIGHSDADVLTHAITDALLGALADGDIGSHFPPSDPQWRGAASSIFLAAAAAKVRSRGGIIAHIDATLVCERPKLGPHRDAIRASIARIAEIPLDRVAIKATTSEGLGFTGREEGIASFAVATVVSRGHPAEEPERVGTGFDVHRLVAGRPLFLCGVEVPHSMGLDGHSDADVGLHALCDAIYGALAEGDIGRHFPPSEMRWKDADSAAFLRHAAGRIAARGGMLANADVTLICERPKITPHAPAMIARVAELLGVPESRVSVKATTTERLGFPGREEGIAAQAAVSVLEAR",
    "response#3": "REQTKKEEHMDIRLGNGYDVHRFGEGDHVILCGVKIPHGRGLQGHSDADVGMHAVTDALYGAMAEGDIGRHFPPSDPQWKGAASEIFLKHAADLARSKGFTISNVDCTLVCEYPKVGPHAAAMMTEMARILGMEMDRVSIKATTSERLGFTGREEGIAAIATATLIERVGAGYDLHRLVEGRPLILGGIHVPADAGAMGHSDADVICHAATDAVLGAACLGDIGRHFPDTDPAWKDASSMDLLGRAVGILERAEFRVVNLDVVVILERPKIAPYIDRIRQALAAVLHVDIDHVSVKGKTNEGVDAVGRGEAIAAHAVALVRAG"
  },
  {
    "reference": "MEMGKAAAVVLAVNGKRYEAAGVDPSTTLLEFLRTHTPVRGPKLGCGEGGCGACVVLVSKYDPATDEVTEFSASSCLTLLHSVDRCSVTTSEGIGNTKDGYHPVQQRLSGFHASQCGFCTPGMCMSIFSALVKADKAANRPAPPAGFSKLTSSEAEKAVSGNLCRCTGYRPIVDACKSFAADVDLEDLGLNCFWKKGDEPADVSKLPGYNSGDVCTFPDFLKSEMKSSIQQANSAPVPVSDDGWYRPRSIDELHRLFQSSSFDENSVKIVASNTGSGVYKDQDLYDKYIDIKGIPELSVINRNDKGIELGSVVSISKAIEVLSDGNLVFRKIAGHLNKVASPFVRNTATIGGNIVMAQRLPFASDIATILLAAGSTVTIQVASKRLCFTLEEFLQQPPCDSRTLLLSIFIPEWGSNDVTFETFRAAPRPLGNAVSYVNSAFLARTSLDAASKDHLIEDICLAFGAYGADHAIRARKVEDYLKGKTVSSSVILEAVRLLKGSIKPSEGSTHPEYRISLAVSFLFTFLSSLANSLNESAKVSGTNEHSPEKQLKLDINDLPIRSRQEIFFTDAYKPVGKAIKKAGVEIQASGEAVYVDDIPAPKDCLYGAFIYSTHPHAHVKSINFKPSLASQKIITVITAKDIPSGGQNVGYSFPMIGEEALFADPVAEFAGQNIGVVIAQTQKYAYMAAKQAIIEYSTENLQPPILTIEDAIERSSFFQTLPFVAPKPVGDYDKGMSEADHKILSAEVKIESQYFFYMEPQVALAIPD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein contains novel 2Fe-2S ferredoxin-type, FAD-binding PCMH-type domains that confer a unique function or activity.2. The protein must be engineered to have a high affinity for Mo-molybdopterin in order to increase its catalytic activity.3. The desired protein should be able to perform H2O + indole-3-acetaldehyde + O2 = (indol-3-yl)acetate + H(+)   + H2O24. The protein should have a high affinity for [2Fe-2S] cluster.5. The protein must exhibit the following characteristics: Involved in the biosynthesis of auxin.; In higher plants aldehyde oxidases (AO) appear to be homo- and heterodimeric assemblies of AO subunits with probably different physiological functions.6. The designed protein should be involved in auxin biosynthetic process, abscisic acid biosynthetic process by localizing to cytoplasm and exhibiting molybdopterin cofactor binding, iron ion binding, indole-3-acetaldehyde oxidase activity, FAD binding, 2 iron, 2 sulfur cluster binding. The designed protein sequence is ",
    "response#1": "MFYINGKKITEKNVDPEEMLLTYLRKKVRLTGTKYACGTGGCGACTVMISRYNPFQEQTMRASHFSVNACLVPICSLHGAAVTTAEGIGSTKTKLHPVQERIAKAHGSQCGFCTPGMVMSMYTLLRNIPEPTMEDIREALTGNLCRCTGYRPIIDGFKTFCGASGCCQDEESTTGCCLDQGPNLNQMDSDVSNELFRMNDVLPLDPTQDLIFPPELMLMAKKQGAQPLCFQGNRIRWISPVDLADLLELKSLYPDAPLVIGNTTVGLNVKLKGVFHPLIIAPGRIAELNMIKEGKYGLTFGAACSLSAMKDELKRAVSELAEEKTKVFQALLQTLSCLAGKQIRNMATLGGNILSAIPKYDLNSILAAADCTLLVVSKGGSREIRLNEEFFTGFGKTTLKPDEVLLTVHIPYSRKWEFVSAFRQAQRREFAFSIVNSGMRVVFEEGTNVVKSLNIFYGGVGPTLIRASYTCQELAGRLWDEKTLQEACRLLGEEISLSPSAPGGRVEFKKTLTLSFFFKFYMED",
    "response#2": "LVFFVNGKKVVEKNADPETTLLAYLRRNLGLSGTKLGCGEGGCGACTVMLSKYDRLQNKIIHFSANACLAPICSLHHVAVTTVEGIGSTKSRLHPVQERIAKSHGSQCGFCTPGMVMSMYTLLRNQPEPTVEEIENSFQGNLCRCTGYRPIIQGFRTFARDGGCCGGNVDNPNCCMNQKKDDTVTESPCLFNPEEFMPLDPTQEPIFPPELLRLKDAPRKQLRFEGERVTWIQASTLKELLDLKAQYPEAKLVVGNTEIGIEMKFKNMLFPVIVSPAWIPELNVVEHGPEGISFGAACPLESVEKTLKDAVAKLPAHKTEVFRGVLEQMRWFAGKQVKSVASIGGNIITASPISDLNPVFMASGAKLTIVSRGTRRTVPMDHTFFPGYRKTLLGPEEILLSIEIPYSREGEFFSAFKQASRREDDIAKVTCGMRVLFQPGTTQVKELALCYGGMADRTISAFKTTRKQLSNFWNEKLLHEVCAGLAEELYLPPDAPGGMVDFRRTLTLSFFFKFYLFEGERVTWIQASTLKELLDLKAQYPEAKLVVGNTEIGIEMKFKNMLFPVIVSPAWIPELNVVEHGPEGISFGAACPLESVEKTLKDAVAKLPAHKTEVFRGVLEQMRWFAGKQVKSVASIGGNIITASPISDLNPVFMASGAKLTIVSRGTRRTVPMDHTFFPGYRKTLLGPEEILLSIEIPYSRKEPYTGRCLLNTRITGDDAPGETWHMVFSTDGEIPYREGQSIGVIPDGIDKNGKPHKLRLYSIASSAIGDFADSKTVSLCVKRLVYTNDQGEIVKGVCSNFLCDLKPGSDVKITGPVGKEML",
    "response#3": "FQGNRIRWISPVDLADLLELKSLYPDAPLVIGNTTVGLNVKLKGVFHPLIIAPGRIAELNMIKEGKYGLTFGAACSLSAMKDELKRAVSELAEEKTKVFQALLQTLSCLAGKQIRNMATLGGNILSAIPKYDLNSILAAADCTLLVVSKGGSREIRLNEEFFTGFGKTTLKPDEVLLTVHIPYSRKNPYTGRCLLNTKITGDDAPGETWHIVFTTEGEVPYREGQSIGVIPEGIDKNGKPHKLRLYSIASSAIGDFGDSKTVSLCVKRLVYTNDGGEIVKGVCSNFLCDLKPGDEAKITGPVGKEMLSAFKQASRREDDIAKVTCGMRVLFQPGTTQVKELALCYGGMADRTISAFKTTRKQLSNFWNEKLLHEVCAGLAEELYLPPDAPGGMVDFRRTLTLSFFFKFYLTKEPYTGRCLLNTRITGDDAPGETWHMVFSTDGEIPYREGQSIGVIPDGIDKNGKPHKLRLYSIASSAIGDFADSKTVSLCVKRLVYTNDQGEIVKGVCSNFLCDLKPGSDVKITGPVGKEMLMLGTGTGIAPFRSFLWKMFFEEHDDYKFNGLAWLFLGVPTSSTLLYREEFERMKEIAPERFRLDFAVSREQTNAAGEKMYIQTRMAEYKDELWELLKKDNTYVYMCGLKGMEKGSAFKQASRREDDIAKVTCGMRVLFQPGTTQVKELALCYGGMADRTISAFKTTRKQLSNFWNEKLLHEVCAGLAEELYLPPDAPGGMVDFRRTLTLSFFFKFYLTTSEGLGNSRDGFHPIHKRLSGFHASQCGFCTPGMSVSLFSALLDADKSQYSDLTVVEAEKAVSGNLCRCTGYRPIVDAC"
  },
  {
    "reference": "MIRKLGIVGVGLIGGSAAMAARRAGFAAEVVGLGRTQENLDTALSRGIVDAASRDPEILADCDLVLLATPVRTLALNAEQIVGQLRPDTVVTDGGSVKVEVVRDCEAILGSRFVGSHPIAGTEDSGAAAADESLFLDAVCVVTPSSETDPAATVLVRQFWEALGMRVVSMDPPSHDRALGVTSHLPHLLAFALAGVAAGERTAIGELLGPSFRDMTRIAASSPEMWRDILLANASTLSDVAGRFAEELEALRLAATRGDGAALESRIRRAGDWKRQTTGGTETTAEIQPSSTPLQGNVRVPGDKSIGHRALLFGGIAQGVTRVRGLSPGADNASTVEVLRGLGIQVDRNGDEALVHGGGFEGLRMPAATLDCGNSGTTMRLCAGLLAGRPFSSRLDGDASLRSRPMARVQGPLAALGAVIETEDGHAPLVVRGRSLKGADVTLSVASAQLKTSVLLAGLQAEGRTVVREPLRSRDHTERLLPHFGVLVEVANDGGIAVQGPVTLRAADVDVPGDPSAAAFWAVAASIVPGSEVHIPDVAMNPTRIGALDVLVAMGADLTRTDKPSVGAEPVADLTVRSAALRGVEVAGETMVRAIDEFPILAVAAAFADGETVFADGAELRHKESDRLATMAAGLQRMGVAVREQPDGLVVEGGGRLAGATVETFGDHRIAMAFAVAALGASGPVRIEDADSIAVSDPRFLAVLETLRAELR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design must be able to perform NAD+ binding, 3-phosphoshikimate 1-carboxyvinyltransferase activity in order to contribute to chorismate biosynthetic process, tyrosine biosynthetic process.2. Ideal protein should have a binding affinity for key enzymes in the chorismate biosynthesis pathway to enhance the overall efficiency of the pathway.3. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.4. Incorporate a signal peptide in the protein design.5. The protein must exhibit the following characteristics: Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate..6. The protein with the reaction 3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-   carboxyvinyl)-3-phosphoshikimate + phosphate, the Proton donor must be optimized to ensure efficient catalysis.7. The designed protein must have at least one Prephenate/arogenate dehydrogenase domain.8. The protein should be able to bind phosphoenolpyruvate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "IALGLRRKFPNLDILAADPDTQALKAARNEGTLTGAGSAEEVIAAADLIVLAMPPLAIPEYLTLLQKHGKPDAVFTDVGSVKSHVLASLVGCEAKLSARFVPGXPIAGSEKSGYVSAKSELFEXRRVILTPHADNTASAVAEIHLMWRALGAEAVLRHVPEPESGQVRLPGSKSISNRTLLLAALAEGETDIRDLLASDDVERMLDALRALGVAWSRDGDTDNYRVTGVGGPFPVKSADLFLGNAGTAFRPLTAALALSGGEYRLSGVPRMHERPIGDLVDGLRQLGAGVDYLANEGFPPLHIRPATIRSGGVVRVRGDVSSQFLTALLMALPLTGVESTVEVVGELISKPYIAITLDLMARFGVEVRREGWERFVVPGGVRYRSPGVIFVEGDASSASYFLAAGAIGGGPVRVEGVGSTSIQGDVRFAEALATLGARIEMGDNWIEARAPESGRLKAFDLDLNHIPDAAMTLAVAALFADGPCRLRNIASWRVKETDRIAAMATELRKVGAVVEEGADYLVVTPPAVLRAAAIDTYDDHRMAMCFSLVALGGCRVRINDPKCVNKTFPTYFEPARHDEVLAATSHLPHLLAYSIVDLLIHQDXSKELFRYAAGGFADFSRIASSNAQMWSDIAIANADATVAILTQYIEYLDELKRLILRRQGQDIKHLFL",
    "response#2": "MIEPIGKINGTVYLPGSKSISNRVLLLSALAAGKTRLTNFLDSDDTRYMLEALKALGVRYTVSKNGTCCEVEGVNGPLNTPHPIELMTGNAGTVMRFLTAVLSLGQSDVVLTGGARMKERPMDPLVDALRQGNAQITYLEKKNSPPLRLRGGFRGGNLTLRGNISSQFLTALLIMAPLAEQDTAIEVQGFLVSKPYVEMTLHLMRVFGISVTHKHYRTFNIKAHQKFKSPGHYRIEGDATAASYFLAAAAIKGGCVRVIGVGQKSIQGDVQFADVLQKMGAFIHWGEDYIECRPGRLKGIDMDMNSMPDAAMTLATTALFSEGPTRIQNIYNWRLKETDRLSAMSAELRKLGAQIKEGQDYIEIISPKNLKAAEIQTYNDHRIAMSFSLMALSDMPIRILNPRCTSKTFPDFFQKLHTLLK",
    "response#3": "IALGLRRKFPNLDILAADPDTQALKAARNEGTLTGAGSAEEVIAAADLIVLAMPPLAIPEYLTLLQKHGKPDAVFTDVGSVKSHVLASLVGCEAKLSARFVPGXPIAGSEKSGYVSAKSELFEXRRVILTPHADNTASAVAEIHLMWRALGAETDICASAASAPARHDEVLAATSHLPHLLAYSIVDLLIHQDXSKELFRYAAGGFADFSRIASSNAQMWSDIAIANADATVAILTQYIEYLDELKRLILRRQGQDIKHLFPARHDEVLAATSHLPHLLAYSIVDLLIHQDXSKELFRYAAGGFADFSRIASSNAQMWSDIAIANADATVAILTQYIEYLDELKRLILRRQGQDIKHLFAPAARR"
  },
  {
    "reference": "MPSIQLPVLLLCLTLSGVCLNGRQFPPELSENMGRSSLDDILQRSGSHMLQSVLKKVEKKEEMNKELNMPLPQWLSKRQHPGKRYISDPEKRQHPGKRDVEEKASFGDIQKRQHLGKTEVEGYLVNYLELKKRQHPGRRSLWDQSTDISSSQLTYLNELSKRQHPGRRYLMYKHQHPSKRGWNDELDLSDQNWEKHQQFGNRDRDSDSPDYTGPCDLQQSAICNKDSLLLDLAEKFSKEGVEEKHQHPGRRSAWENETEE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems.2. The ideal protein would be one that plays a critical role in negative regulation of glutamate secretion, eating behavior, histamine metabolic process through thyrotropin-releasing hormone activity within plasma membrane.3. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "LLKLKVMYTVGYSSSLAMLLVALSILCSFRRLHCTRNYIHMHLFVSFILRALSNFIKDAVLFSSDDVTYCDAHKVGCKLVMIFFQYCIMANYAWLLVEGLYLHTLLAISFFSERKYLQAFVLLGWGSPAIFVALWAITRHFLENTGCWDINANASVWWVIRGPVILSILINFIFFINILRILMRKLRTQETRGSETNHYKRLAKSTLLLIPLFGIHYIVFAFSPEDAMEVQLFFELALGSFQGLVVAVLYCFLNGEVQLEVQKKWRR",
    "response#2": "LLKLKVMYTVGYSSSLVMLLVALGILCAFRRLHCTRNYIHMHLFLSFILRALSNFIKDAVLFSSDDAIHCDAHRVGCKLVMVFFQYCIMANYAWLLVEGLYLHSLLVVSFFSERKCLQGFVVLGWGSPAMFVTSWAVTRHFLEDSGCWDINANAAIWWVIRGPVILSILINFILFINILRILTRKLRTQETRGQDMNHYKRLARSTLLLIPLFGVHYIVFVFSPEGAMEIQLFFELALGSFQGLVVAVLYCFLNGEVQLEVQKKWQR",
    "response#3": "LLKLKVMYTVGYSSSLAMLLVALSILCSFRRLHCTRNYIHMHLFVSFILRALSNFIKDAVLFSSDDVTYCDAHKVGCKLVMIFFQYCIMANYAWLLVEGLYLHTLLAISFFSERKYLQAFVLLGWGSPAIFVALWAITRHFLENTGCWDINANASVWWVIRGPVILSILINFIFFINILRILMRKLRTQETRGSETNHYKRLAKSTLLLIPLFGIHYIVFAFSPEDAMEVQLFFELALGSFQGLVVAVLYCFLNGEVQLEVQKKWRH"
  },
  {
    "reference": "MLTDKRILLIIAGGIAAYKSLDLIRRLRERGAWVTPVLTRAAEEFVTPLSVSSLAGEKVYRDLFDLTDEAEMGHIQLSRSADLIVVAPATADLLAKMANGMANDLASTLLLATDTPVLTAPAMNVRMWEHPATQRNLETLQNDGVGFVGPNEGDMACGEYGPGRMSEPLEIVAAIEGAMKVGPLSGRRVLVTSGPTHEPIDPVRYIANRSSGAQGTAIAEAMVAQGAEVVFVTGPAEAGRPHGVHLVEVETAQEMMQAVQGALPVDAAIFSAAVADWRVASASDSKIKKTKQGLPTLEFAENPDILATVSQMAMGRPKLVVGFAAETDDVIDNATSKRERKGCDWIVANDVSPETGIMGGTENAVTLISDSGAEAWPRMGKRDVARLLAERVAKAL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal domain. The designed protein sequence is ",
    "response#1": "MRILLGITGGIAAYKAANLIRGFSELGHEVTAVPTENALRFIGKPTLEALSGHPVETDMYVDVDMVRHVELGQQADLIVIAPATASFIARLAAGIADDLLLNAVLASKAPVVLAPAMHTEMWQNSATRANIETLAQRGIRVLEPSSGRLTGADSGPGRLPETNEIISGL",
    "response#2": "MRILLGITGGIAAYKAANLIRGFSELGHEVTAVPTENALRFIGKPTLEALSGHPVETDMYVDVDMVRHVELGQQADLIVIAPATASFIARLAAGIADDLLLNAVLASKAPVVLAPAMHTEMWQNSATRANIETLAQRGIRVLEPSSGRLTGADSGPGRLPETNEIISQ",
    "response#3": "LTGETVLVTAGPTREELDPMRFLSNHSSGKMGYAIARAAADRGARVRLVAGPTALSTPRGVELRQVVSAREMHAAVMDLASDCSIVVKAAAVADYRPACRQDQKIKKGGESTLSLELQRNPDILAELGQLQGERFLVGFAAETEQLLENARRKLQDKNLDLIVANDLTAEGAGFAGDTNVVRLIG"
  },
  {
    "reference": "MEPKIRRILNKELQRQRDCICLIASENYVSRDILEVTGSILTNKYAEGYPTRRFYEGCEVVDESESLAINTCKELFGAKWANVQPHSGSSANYAVYLALLKPGDAILGLDLNCGGHLTHGNKFNFSGKQYQPYSYTINPETEMLDYDEVLRVAREVKPKLIICGFSNYSRTVDFERFSAIAKEVGAYLLADIAHIAGLVAAGLHPNPLPYTDVVTSTTHKTLRGPRGGLIMSNNEAIIRKLDSGVFPGCQGGPLQHVIAAKYVCFKEALQPKYKQYIQNVKTNAASMASWFKQQGYRVISNGTDTHLFSLDVGKGKDVSQWLQQANIVLNMNTVPFDKNPAINPSGIRIGTPAMTTRGFKEKHFLYVAALIDKIIKSDGNKKVIKEVKKAVLKLLERFPLYKGLEY",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm that is critical for tetrahydrofolate interconversion.2. The protein should be optimized for activity in Amino-acid biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. For general function, the protein need meet that This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier4. The (6S)-5,6,7,8-tetrahydrofolate binding site should be stable and able to withstand changes in environmental conditions.5. The protein should have a low Km value for (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O =   (6S)-5,6,7,8-tetrahydrofolate + L-serine. The designed protein sequence is ",
    "response#1": "LEMVDPAVADAIRSERQRQQENIELIASENFTSPAVLEAQGSVLTNKYAEGYPRRRWYGGCEHVDTVEQLAIDRAKKLFGAEHANVQPHSGSSANMAVYFAFLKPGDRMLTMDLSHGGHLTHGNKANFSGRFFEIIHYGVRQEDEQIDYLQLARMAQEHRPRMITVGASAYPRIIDFRRMGEIARAAGALLLADIAHIAGLVAAGLHPSPIEHADFVTTTTHKTLRGPRGGLILCAEKYAKEIDSQVFPGIQGGPLMQVIAAKAVCFHEALQPAFREYQQQIVKNAKALAEGMKRNGFRLISGGTDSHLMLVDVGSRVVTGKDCQVALDEAGITVNKNTIPFETRSPFQASGVRLGTPAVTTRGMKEPEMAAIADMIVEVLDAVKTGDQETAKGDPEVYEIVMKELGRQEEGLELIASENFVSPAVMEAMGSTLTNKYAEGYPRRRYYGGCKFVDEAEQLARERVKKLFNCKYANVQPHSGSQANMAAYLAVANPGDTIMGMSLSHGGHLTHGSPVNFSGKLFNIVSYGVDLETEVLDYDEIERLALEHKPKIIIAGGSAYSRIIDFKRFREIADKVGAYLIVDMAHFAGLVAAGLYPNPVDHAHIVTSTTHKTLRGPRGGLILTNDEELYKAINKAVFPGIQGGPLMHVIAAKAVAFGEALKDEFKEYQMRIITNAKALAKELENLGLRIVSGGTDTHLFLVDLNPKNVTGKAAEKALESADITVNKNTIPKETRSPFVTSGIRIGTPAVTTRGMGESEMKVIAELIADVIDVVKDPADVEAVKVQVKELTSKFPVYE",
    "response#2": "LAQADPEVAEYVDRELRRQQETLEMIASENFASVAAMQAQGSVLTNKYAEGYPGRRYYGGCENVDVIEQLAIDRIKGLFGAEFANVQPHSGAQANAAAMQALIKPGDTILGLSLAHGGHLTHGMRINYSGLLYDVAAYEVSKEDYRIDMDEVSRMARQHQPKLIIAGWSAYPRHLDFARFREIADEVGAYLMVDMAHFAGLVATGLHPSPVPHAHVVTSTTHKTLGGPRGGIILSNDAAIAKKINSAVFPGQQGGPLEHVIAAKAVAFKMAGDPAFVDRAERTLLGARLLAERWVQADVADAGIKVLTGGTDVHLVLVDLRDSELDGKAAEDRLHSVGITVNRNAVPFDPRPPMVTSGLRIGTPALATRGFDARDFVEVADIIADALGTLADTDDLEGARVRQMLDYVTCADLSALKEGESTAVCLETPEGHVEGALTCVTWDHYRLSLPAKQAAATVTWLRDLSDGYVSLRLDGAPDYSAMRIPGDLEGARVRQMLDYVTCADLSALKEGESTAVCLETPEGHVEGALTCVTWDHYRLSLPAKQAAATVTWLRDLSDGYVSLRLDGAPDYSAMRIPGR",
    "response#3": "MTNKYAEGYPRARYYGGCECVDIVEDLARDRLKEIFGAKHANVQPHSGAQANMAVYLAELNVGDTVLGMNLSHGGHLSHGSKVNSSGKLYNFIPYGVDEKTGKIDYEEVERLAIEHKPKLILAGASAYPREIDFKKFKEIADKVGSLFMVDMAHIAGLVAAGLHQNPVPYADFVTSTTHKTLRGPRGGIILCKEKYAKEIDKAVFPGIQGGPLMHVIAAKAVCFGEAQKPEFKEYQNQIIKNSKTLAEELLKYGFNLVSGGTDNHLVLIDLRNKGIKGKELETRLDDIGITVNKNAVPFDTEKPSITSGIRIGTPAVTTRGFKEGEMKKIAKLIADVLDDISNEENLSKAKHEVYKLTQKYPIYD"
  },
  {
    "reference": "MTRLDNSRVIRPATGPEISAKSWLTEAPMRMLMNNLHPDVAEAPHELVVYGGIGRAARDWESYDRIVETLRRLEGDETLLIQSGKPVGVFRTHADAPRVLLANSNLVPQWANWEHFHELDKKGLMMYGQMTAGSWIYIGSQGIVQGTYETFVEMGRQHYGGDLSGRWLLTAGLGGMGGAQPLAAVMAGASCLAIECQPSRIEMRLRTGYLDRQAASIDEALAMIEASHAEDKPVSVGLLGNAAEILPEIVRRGIRPDLLTDQTSAHDPVNGYLPAGWSLDQWFAKRESDPSAVAKAAKASMAVHVRAMLDLHAAGVPTTDYGNNIRQMAKDEGVENAFDFPGFVPAYVRPLFCRGIGPFRWVALSGDPEDIYRTDARVKQLLPDNTHLHNWLDMARERIQFQGLPARICWVGLGDRHRLGLAFNEMVASGELKAPIVIGRDHLDSGSVASPNRETEAMRDGSDAVSDWPLLNALLNTASGATWVSLHHGGGVGMGYSQHSGMVIVADGTPEAAKRLERVLWNDPGTGVMRHADAGYDIAIDCAREKGLDLPSI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be optimized for urocanate hydratase activity. The designed protein sequence is ",
    "response#1": "GSHLAMSNMFAYGRLAWNPRADSRDIVEEWTRLTFGLDRDVVSTIADMSLKSWPAYEGYSGNLGIQTLTDILYTHYGANPASQDNNGWGQWTRADSKTIGMDRTVSNGTGNAGQYPKEVAARFEHTQTTPDDLMLWFHHVPYTFRLHSGKSVIQHFYDAHYTGAATVQRFPAAWKSLKSKIDTERYNAVLYKLQYQTGHSLVWRDAITEFYRNLSSIPDQLNIW",
    "response#2": "MPEDVREWLPRRPADTHKRAVGTLLIIGGAPTFYGAPRLAAGAALRAGAGLVTLAVIRSLISPIASSLPEVTFLPLPEGELGAGVRMAELVREALPRYDAVLIGPGLGQEPPVPEFLARLFGLRGVPRPIGFGQPAAAGLPQRFRGRAVIDADGLNWLATQPEWWVQIREADLILTPHPGELARLLQVETDAVTADPWGRAAEAAQRFNQHVVLKYGHATVGCPDGTLLVSPQVHPALATAGTGDVLAGLIAALAAQGLGSREAAAAGLVVGSQAMLRAVARTGTLGLIAGDIIEEIAPALAALA",
    "response#3": "GSHLAMSNMFAYGRLAWNPRADSRDIVEEWTRLTFGLDRDVVSTIADMSLKSWPAYEGYSGNLGIQTLTDILYTHYGANPASQDNNGWGQWTRADSKTIGMDRTVSNGTGNAGQYPKEVAARFEHTQTTPDDLMLWFHHVPYTFRLHSGKSVIQHFYDAHYTGAATVQRFPAAWKSLKSKIDTERYNAVLYKLQYQTGHSLVWRDAITEFYRNLSSIPDQLNR"
  },
  {
    "reference": "MYAIVENGGKQYKVEVGHLLHTEKMNGVAQGDKVVLDKVVLVKTDDGKVLVGKPYLTNVTITGIVVEHARARKILVGQFIPRKGHKTIKGHRQWYTTIKIENIEIK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be involved in translation by localizing to ribosome and exhibiting rRNA binding. The designed protein sequence is ",
    "response#1": "RGGVLNFGQFGLQALECGFVTNRQIEAARIAMTRHVKRGGKIWIRIFPDKPFTKKPAEVRMGKGKGAPEGWVAVIKPGRILYEMDGVSYELAAEALRLAAHKLPIKTKII",
    "response#2": "SLKLQKRLAASVLRCGKKKVWLDPNEINEIANTNSRQNIRKLIKDGLIIKKPVVVHSRYRVRKNTEARRKGRHCGFGKRKGTANARMPTKLLWMQRQRVLRRLLKKYRDSKKIDRHLYHDLYMKCKGNVFKNKRVLMEYIHKKR",
    "response#3": "RGGVLNFGQFGLQALECGFVTNRQIEAARIAMTRHVKRGGKIWIRIFPDKPFTKKPAEVRMGKGKGAPEGWVAVIKPGRILYEMDGVSYELAAEALRLAAHKLPIKTKIR"
  },
  {
    "reference": "MPHITIEEAIDDIKNGKMVILVDDEDRENEGDLTMAADAVTPEAINFMAKYGRGLICLSLDGPLVDRLNLPMMVEDNESPFETGFTVSIEARKGVTTGISAKDRAVTIQAAIADDAKPTDLVRPGHIFPLRARKGGVMVRIGQTEGSVDLARLAGRKPAGVICEIMDEDGTMSRMPSLEKFSKAHGIGICTVADLVAYRKRTESFVRKAAETVIPTLYGGDFRIIAYENDLDNLTHIAMIKGEVKPEEPILVRVHSECMTGDIFGSMRCDCGDQLHTAMEMINQEGAGVILYLRQEGRGIGLVNKLKAYELQRAGLDTVQANEELGFKADLRDYGIGAQILTDLGVRQMRLLTNNPKKMVGLEGYGLSVVEQVPIAIEPNEFNECYLSCKQAKMGHLLNIHPAKTRRR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein is the optimization of the Nucleophile; for GTP cyclohydrolase activity to achieve high catalytic efficiency for GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.2. The protein design must be able to perform magnesium ion binding, GTP cyclohydrolase II activity, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, manganese ion binding in order to contribute to riboflavin biosynthetic process.3. The designed protein have GTP cyclohydrolase II domains with specific sequence motifs that are crucial for its activity.4. The Zn(2+), Mg(2+), GTP binding site should be located in a region of the protein that is accessible to the ligand.5. The protein should be optimized for activity in Cofactor biosynthesis, riboflavin biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "REVDIQLPTAFGDFRAVGFSDIIDGKESVALIKGEVIEGEPTLVRVHSECLTGDVFGSHRCDCGPQLHAALTQIEQEGRGILLYMRQEGRGIGLMNKLKAYKLQEEGYDTVEANEKLGFPADLRDYGIGAQILRELGVRQMRLLTNNPRKITGLKGYELEVVERVPYVSVPEVPEREVDIQLPTAFGDFRAVGFSDIIDGKESVALIKGEVIEGEPTLVRVHSECLTGDVFGSHRCDCGPQLHAALTQIEQEGRGILLYMRQEGRGIGLMNKLKAYKLQEEGYDTVEANEKLGFPADLRDYGIGAQILRELGVRQMRLLTNNPRKITGLKGYELEVVERVPYVSVPEIPEYREVEIKLPTEYGAFRAVGYTSIIDQKEHVAIIKGDISGAEPVLVRVHSECLTGDVFGSNRCDCGPQLEAALTQIEAEGRGVLLYMRQEGRGIGLINKLKAYKLQEEGYDTVEANNKLGFAADLRDYGIGAQILRELGITKMRLLTNNPRKIAGLNGYGLEVIERVP",
    "response#2": "TSAMPTEFGDFRATAYRNTLDGTEHLALVMGDLAAASRTTEGALVRVHSECLTGDILGSLRCDCGGQLEQALAAIAAEGAGAVVYLRGHEGRGIGLGHKIRAYALQEQGLDTVDANTAQGLPVDSRSYGVGAQILGDLGATRIRLITNNPAKYGGLDGYGLTITGRETQMPVRSGGEFTAYAYRNTMNGDTHIAMVKGEIRPEVPVLVRVHSECLTGDVFGSIRCDCGEQLRNALQRIETEGTGILLYMRQEGRGIGLENKIRAYRLQDRGFDTVEANQRLGFKPDLRDYGVGAQILVDLGVREIRLLTNNPKKIIGLEGYGLRVVERIPQYPEGTTSAMPTEFGDFRATAYRNTLDGTEHLALVMGDLAAASRTTEGALVRVHSECLTGDILGSLRCDCGGQLEQALAAIAAEGAGAVVYLRGHEGRGIGLGHKIRAYALQEQGLDTVDANTAQGLPVDSRSYGVGAQILGDLGATRIRLITNNPAKYGGLDGYGLTITGRETQMPVRSGGEFTAYAYRNTMNGDTHIAMVKGEIRPEVPVLVRVHSECLTGDVFGSIRCDCGEQLRNALQRIETEGTGILLYMRQEGRGIGLENKIRAYRLQDRGFDTVEANQRLGFKPDLRDYGVGAQILVDLGVREIRLLTNNPKKIIGLEGYGLRVVERIPQYPEGT",
    "response#3": "TSAMPTEFGDFRATAYRNTLDGTEHLALVMGDLAAASRTTEGALVRVHSECLTGDILGSLRCDCGGQLEQALAAIAAEGAGAVVYLRGHEGRGIGLGHKIRAYALQEQGLDTVDANTAQGLPVDSRSYGVGAQILGDLGATRIRLITNNPAKYGGLDGYGLTITGRLMAEHAKIKLPTRFGEFDMYGFTSKVDGSENLAIVKGQLSEEMNVRIHSACATGDIFHSQRCDCGEQLEVAMSYIQENDGIILYLPQEGRGIGLINKLKAYELIEQGYNTVTANEALGFEPDLREYDIAAQMLKHLGVRKINLLSNNPEKFSGLRDYGIEILDRIPTHPFPGPGLGIRVVDIPTQFGNFTFAGYSDEIEHKEYIAVMKGEIKNKENVTVRLHSECLTGDVFGSRRCDCQEQLHRALHELEESGEGLIIYLRQEGRGIGILNKLKAYKLQDEGYDTVEANHKLGFSDDLRDYAVAAQIIKDLGIKSIILKTNNPKKIEGLEKYGVKVAGRREVDIQLPTAFGDFRAVGFSDIIDGKESVALIKGEVIEGEPTLVRVHSECLTGDVFGSHRCDCGPQLHAALTQIEQEGRGILLYMRQEGRGIGLMNKLKAYKLQEEGYDTVEANEKLGFPADLRDYGIGAQILRELGVRQMRLLTNNPRKITGLKGYELEVVERVE"
  },
  {
    "reference": "MAKSFPVFSLLSFILIHLVLSSVSGPRHWWPPRGIIKVKCPYEKVNLSWYNGTVNPCPGLYQPICGTNFITYDNPCILCVESLKSHGRIRFYHDGKC",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that May be a serine protease inhibitor.2. The designed protein must be able to regulate negative regulation of peptidase activity.3. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "LHLYQSVAACRNETNFVISPAGVSLPLEILQFGAEGSTGQQLADALGYTVHDKRVKDFLHAVYATLPTSSQGTEMELACSLFVQVGTPLSPCFVEHVSWWANSSLEPADLSEPNSTAIQTSEGASRETAGGGPSEGPGGWPWEQVSAAFAQLVLVSTMSFQGTWRKRFSSTDTQILPFTCAYGLVLQVPMMHQTTEVNYGQFQDTAGHQVGVLELPYLGSAVSLFLVLPRDKDTPLSHIEPHLTASTIHLWTTSLRRARMDVFLPRFRIQNQFNLKSILNSWGVTDLFDPLKANLKGISGQDGFYVSEAIHKAKIEVLEEGTKASGATALLLLKRSRIPIFKADRPFIYFLREPNTGITVFFDRIQIN",
    "response#2": "SMYRLNTYPFIHVGFFHAFVNLLALTPLLERFEAEHGTLTAVALFIGPLSTFPAGIYILVEKFILRSNTAVVGASVWIFLLLGSEAIKTFKSNPYFSLGTTKIPTWTSPLFACALVSIFVPNTSFLGHLSAIIIGYLLGLGYF",
    "response#3": "LHLYQSVAACRNETNFVISPAGVSLPLEILQFGAEGSTGQQLADALGYTVHDKRVKDFLHAVYATLPTSSQGTEMELACSLFVQVGTPLSPCFVEHVSWWANSSLEPADLSEPNSTAIQTSEGASRETAGGGPSEGPGGWPWEQVSAAFAQLVLVSTMSFQGTWRKRFSSTDTQILPFTCAYGLVLQVPMMHQTTEVNYGQFQDTAGHQVGVLELPYLGSAVSLFLVLPRDKDTPLSHIEPHLTASTIHLWTTSLRRARMDVFLPRFRIQNQFNLKSILNSWGVTDLFDPLKANLKGISGQDGFYVSEAIHKAKIEVLEEGTKASGATALLLLKRSRIPIFKADRPFIYFLREPNTGITVFFDRIQIN"
  },
  {
    "reference": "MSQQTTPNAPGWAPDSWRSKPIKQCPEYPDKAALEKATNELKTLPPIVLPNEIIRLREHLRDVAQGKAFLLQGGDCAELFSYCQQDVIESKIKLLLQMSLVLLWGADKPVVRIGRMAGQYAKPRSSPVETINGKEVPSFRGDILNGFHPDERELDPNRLVRAYQYSSATLNYIRGAIGSGIADLHGPLDWGLGHVRDPALKSKYQETVDRIQEMLRFMHTIGADQNEKLSTVELFTSHEGLLLEYEEPLTRLLNHPSVRSYPPDSTTPPKKEYYNTSAHFLWIGDRTRQIDHAHVEYFRGIANPIGVKIGPSTPTSDLLPMLRTLNPNREPGKVTLITRYGADKVASLLPAHIRTVESSEYARTVVWQCDPMHGNTQSVSGGIKTRKFSDIFSELQQTLRIHKEEKSYLGGMHLELTGDAVTECLGGGAGLDEDDLSTNYTSFCDPRLNEKQALELAFLVADHYRQERKEKEAERRKSSVV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein with 3-deoxy-7-phosphoheptulonate synthase activity functions is required to enable aromatic amino acid family biosynthetic process, amino acid biosynthetic process.2. The designed protein should interact with enzymes and substrates within the chorismate biosynthesis, chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7 pathway to enhance or regulate its activity.3. The protein should have a fast turnover rate for D-erythrose 4-phosphate + H2O + phosphoenolpyruvate = 7-   phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate. The designed protein sequence is ",
    "response#1": "CILTGEDHRLLVVIGPCSLHDPKAALEYAERLNALRRRYEDRLEIVMRAYFEKPRTVVGWKGLISDPDLDGSYDVNRGIGIARQLLIDINALGLPTATEFLDMVIGQFIADLISWGAIGARTTESQIHREMASALSCPVGFKNGTDGNVQIAVDAIRAARASHMFLSPDKLGQMTIYQTSGNPHGHVILRGGKQPNYHASDVAAAAESLSAFNLPQQLVIDFSHGNCLKQHRRQMDVAAEVAEQIKAGSQAVAGVMIESFLEEGNQKVVSGEPLVYGKSITDPCLGWQESEKLIELAKELC",
    "response#2": "SEVKKTYELPGDKSVGHRSLLIGALPKGEYKIRNFPQSRDCLTTLKIMEELGVKVKVLKDYILVNSPGYENFKKKIDYIDCGNSGTTSRLIAGILAGVGVETNLVGDKSLSIRPMKRIVDPLNSMGANIEMEKDHMPLIFKGNGELKGIDYTMEIASAQVKSCILLAGFLSEGVTKVRELSPTRDHTERMLKYIEGNIKIENKEIEIENSTIKSKDIYVPGDISSAAYIIACAILGEDCEIILENVLLNENRRKYLDLLKKMGANLKYLEKNQCNGEHVGNILVKSSFLKGISIGKEITPYIIDEIPIISLIASFAEGKTIFENVEELKYKESDRIKAIMVNLKSLGVKTELVENNLIIYGGLSKINKEINIRTFNDHRIALTFLCSAMRNSEKTYIDNWDCVAISFPG",
    "response#3": "SEVKKTYELPGDKSVGHRSLLIGALPKGEYKIRNFPQSRDCLTTLKIMEELGVKVKVLKDYILVNSPGYENFKKKIDYIDCGNSGTTSRLIAGILAGVGVETNLVGDKSLSIRPMKRIVDPLNSMGANIEMEKDHMPLIFKGNGELKGIDYTMEIASAQVKSCILLAGFLSEGVTKVRELSPTRDHTERMLKYIEGNIKIENKEIEIENSTIKSKDIYVPGDISSAAYIIACAILGEDCEIILENVLLNENRRKYLDLLKKMGANLKYLEKNQCNGEHVGNILVKSSFLKGISIGKEITPYIIDEIPIISLIASFAEGKTIFENVEELKYKESDRIKAIMVNLKSLGVKTELVENNLIIYGGLSKINKEINIRTFNDHRIALTFLCSAMRNSEKTYIDNWDCVAISFPFL"
  },
  {
    "reference": "MEIEEIKVQTEVNGAPFIIETGYFAKQANGAVIVRQGDTAVLVAAVMSEEPQADIDFLPLTVEYREKYYAYGKIPGGFVKREGKPTDREILVARNIDRPIRPLFPKGFYNDIVITAYTLSADDKYDPDVLGIVGASAALHISDIPFEGPIAGVRVCRVNGEFIVNPTYQQKAQADIEIVVAGSKDAIVMVEGGAKEVPEEDILEAMMFGHQEIKKLIELQEELRLKCGKEKIQVAIDEEEIILKDKLKEFSYEKIKEAFKITDKKQRNKTINAIFEEAFKTLEIPEEKIKKASFIYKDIVSNVMRDNILNEGIRIDGRKPEEIRPIWIKVGVFPRNHGSAIFTRGQTQAFVTVTLGSLSEGQIEESIEAGEVMKRFMLHYNFPPFSTGEAKPPRATSRREIGHGNLAERALEPLIPPEEEFPYAIRVVSDILESNGSTSMATVCGGSLALFDAGVPMKKHVAGIAMGLIKAEDKFVILSDILGDEDHLGDMDFKVAGTRDGVTSIQMDIKVKGLTKEILEKALQQARDGRNYILDLMYQAIPEPRKEVSPYAPTVTTLRVLPDKIPIIIGPAGKNIKKIIEETKVKIDLDPEGLVKIYATSKEAAEKAVSMINELILDVEVGEVYMGKVTRVEDYGAFVELLPGRLSLLHVSQITGERLKSAKEKIKVGDVLKVKVSEIDDQGRIKVSLKDVPENVEPKNKFLFKEE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein have enhanced phosphate + RNA(n+1) = a ribonucleoside 5'-diphosphate +   RNA(n).2. The protein must exhibit the following characteristics: Involved in mRNA degradation.3. The protein can be expressed in cytoplasm and perform magnesium ion binding, RNA binding without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "LGAFISDACKARLKELAYTALAKEERAERTKEIYGETLKSAVEHFSAAFSSEDLAADPSKALCTNEHIIEEEIHSVEKDVMRRMILDDAKRLDGRV",
    "response#2": "LGAFISDACKARLKELAYTALAKEERAERTKEIYGETLKSAVEHFSAAFSSEDLAADPSKALCTNEHIIEEEIHSVEKDVMRRMILDDAKRLDGRM",
    "response#3": "LGAFISDACKARLKELAYTALAKEERAERTKEIYGETLKSAVEHFSAAFSSEDLAADPSKALCTNEHIIEEEIHSVEKDVMRRMILDDAKRLDGRM"
  },
  {
    "reference": "MTGPLLQLTRVTRRFPAGEKDVVVLDDVSLSIDAGEIVAIVGASGSGKSTLMNILGCLDHPSSGSYTVGGRETSELESDELARLRREHFGFIFQRYHLLPHLCAAENVEMPAVYAGSAQAQRRERALALLARLGLSDRASHRPSQLSGGQQQRVSIARALMNGGEVILADEPTGALDSKSGRDVIRVLRELNALGHTVIIVTHDEQVAAHARRIIEISDGRIVGDRLNPHADAADAAPDASGGAQPQRARRLSAGVGRFAEAFRMAWIALVSHRLRTLLTMLGIIIGITSVVSIVAIGEGAKRYMLDEIGSIGTNTINVYPGADWGDSRADAIQTLVAADAAALADQIYIDSATPETSRSLLLRYRNVDVNALVSGVGERFFQVRGMKLAQGIAFGADEVRRQAQVAVIDENTRRKLFGANPNPLGEVILIDNLPCVVIGVTASKKSAFGDMKNLNVWVPYTTASGRLFGQRHLDSITVRVRDGQPSDAAERSLTKLMLQRHGRKDFFTYNMDSVVKTVEKTGQSLTLLLSLIAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARQTDIMQQFLVEAVTVCLMGGAIGIVLSFGMSFVFSLFVDQWKMVFSAASIASAFLCSTLIGVVFGFMPARNASRLDPIDALARD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein contains novel ABC transporter domains that confer a unique function or activity.2. For general function, the protein need meet that Non-canonical ABC transporter that contains transmembrane domains (TMD), which form a pore in the inner membrane, and an ATP- binding domain (NBD), which is responsible for energy generation The designed protein sequence is ",
    "response#1": "ITIISTGLIFGMTLVLTGLANGFRVEARHTVDSMGVDVFVVRSGAAGPFLGSIPFPDVDLARVAAEPGVMAAAPLGSVGTIMKEGTSTRNVTVFGAPEHGPGMPRVSEGRSPSKPDEVAASSTMGRHLGDTVEVGARRLRVVGIVPNSTALAKIPNVFLTTEGLQG",
    "response#2": "PGSTVAIVGPTGAGKTTLVNLLMRFYEIGSGRILIDGVDIASVSRQSLRSRIGMVLQDTWLYDGTIAENIAYGRPEATTDEIVEAARAAHVDRFVNTLPAGYQTRVSGDGGSISVGEKQLITIARAFLARPQLLILDEATSSVDTRTELLIQRAMRELRRDRTSFIIAHRLSAR",
    "response#3": "PGSTVAIVGPTGAGKTTLVNLLMRFYEIGSGRILIDGVDIASVSRQSLRSRIGMVLQDTWLYDGTIAENIAYGRPEATTDEIVEAARAAHVDRFVNTLPAGYQTRVSGDGGSISVGEKQLITIARAFLARPQLLILDEATSSVDTRTELLIQRAMRELRRDRTSFIIAHRLSR"
  },
  {
    "reference": "MYRRLASGLYQTSQRRIAQVQPKSVFVPETIVTTLPNGFRVATENTGGSTATIGVFIDAGSRYENEKNNGTAHFLEHMAFKGTPRRTRMGLELEVENIGAHLNAYTSRESTTYYAKCFTEKLDQSVDILSDILLNSSLATKDIEAERGVIIREMEEVAQNFQEVVFDILHADVFKGNPLSYTILGPIELIQTINKNDLQGYINTHYRSGRMVLAAAGGVNHDAIVKMAEKYFGELKHGDSSTEFVPATYSPCEVRGDIPDLPMLYGAMVVEGVSWTHEDNLALMVANTLMGEYDRMRGFGVNAPTRLAEKLSQDAGIEVFQSFNTCYKETGLVGTYFVAAPESIDNLIDSVLQQWVWLANNIDEAAVDRAKRSLHTNLLLMLDGSTPVCEDIGRQLLCYGRRIPTPELHARIESITVQQLRDVCRRVFLEGQVSAAVVGKTQYWPVNEEIHGRLIRMQ",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.2. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein should have metal ion binding, metalloendopeptidase activity within mitochondrial processing peptidase complex, mitochondrion to regulate protein processing involved in protein targeting to mitochondrion. The designed protein sequence is ",
    "response#1": "MSPDEQHYDKEEQLEEEKLTQKVKALSEEERKQIYEKGLELISLQSKPQDFSCLPALKVSDIEPQIPLTDLEIAYAGDVPVQYCTQPTNGMVYFRAVSSLNTLPEELKPYVPLFCSVITKLGCGVYNYREQAQQMELTTGGMSVCPHIISDDSSLDTYEQGILFSSLCLDRNMPDMMHLWSEIFNSPHFDDEERLRVLVRMSAQEMSNGIPDSGHVYASIRASRTLTPTGELQELFSGMDQVKMIKRR",
    "response#2": "MRAAPNEEPRRKLYLAANSSTPEQIHVLETLLKKRAELAQLVGRDSFAHMTLDDKMAKKPEHVTNFLDALIDHTRPFARNALRTLAQRKQAHHNLPALPVIQAWDRDFYCPPDPPAPPIPLPPLTIGTVFMGLSRLFRHLYGVSLRPAQAASGEVWHPDVQKLEVVDEQQGIIGWIYADLFPRRGKASGAAHYTVRCSRRTDDDDEANDGMFEGTELQIQESQQFEAVKRHRLPNQEGVYQLPLVVLLTEFARPSLSKGAAVLEWHEVQTLFHEMGHAMHSMLGRTEYQNVSGTRCATDFVELPSILMEHFLNSPAVLSLFDADNTTSLRQIGNHHNDPCHAIDTYSQIMLAVVDQVYHSPSVLNSSFDSTNEFANLVNKRGLIPYVPGTSFQTQFGHLFGYGATYYSYLFDRAIASRVWSKVFSRDPLNRELGEQYKQEVLRWGGARDPWEMVSTLLDQPELAAGDAEAMES",
    "response#3": "MSPDEQHYDKEEQLEEEKLTQKVKALSEEERKQIYEKGLELISLQSKPQDFSCLPALKVSDIEPQIPLTDLEIAYAGDVPVQYCTQPTNGMVYFRAVSSLNTLPEELKPYVPLFCSVITKLGCGVYNYREQAQQMELTTGGMSVCPHIISDDSSLDTYEQGILFSSLCLDRNMPDMMHLWSEIFNSPHFDDEERLRVLVRMSAQEMSNGIPDSGHVYASIRASRTLTPTGELQELFSGMDQVKMIKRC"
  },
  {
    "reference": "MEITEDGVCIGNFRAYGVREGKYGVALILNDEVCESVGVFTKNKVNGASVPIDKEKLKRGGIRAVIANSGNANCCTKTALEDAKRMCEIAGEKLGIDADSILVASTGIIGKKLDVLKIKELVDKIDREHEGSSNASKAIMTTDTYPKQISVNYRGIKIGGVAKGAGMIAPDLATMLCFLTTNADFGKEELQTALENAVEDSFNMLSVDGDMSTNDTVILMSNRSHECKFEDFCYALNFVARELAKLIAKDGEGATKFLEIEVKNAKSRENARKGARAIISSPLVKTAIFGENPNWGRIVSALGSVIEVDFDKIDIIFSNDKDKTKVVEKGVTGDLEKAHEILKSREIRIVVNLHDGDENAVAYGCDLTYSYVKINAEYN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. We are looking for a protein with broad substrate specificity for acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate.2. The protein design should take into account the physiological conditions of the L-arginine biosynthesis pathway.3. The designed protein should have a binding site that is specific to the target substrate ligand. The designed protein sequence is ",
    "response#1": "TTFVKRGMPVSLIPDPRVQVWTAQTRPNMNLDDPHVDLPRLVHLIEDSFNRKLDVKDYLNRVNGRLAGLIIAGEYEGGAILTWELPPGVEDDGSESSTARMVPYLDKFAVLKRSQGAGGVADIVFNAMVRTCFPNGVCWRSRKDNPVNKWYFERSLGSWKLADTNWTMFWTC",
    "response#2": "TTFVKRGMPVSLIPDPRVQVWTAQTRPNMNLDDPHVDLPRLVHLIEDSFNRKLDVKDYLNRVNGRLAGLIIAGEYEGGAILTWELPPGVEDDGSESSTARMVPYLDKFAVLKRSQGAGGVADIVFNAMVRTCFPNGVCWRSRKDNPVNKWYFERSLGSWKLADTNWTMFWTC",
    "response#3": "TTFVKRGMPVSLIPDPRVQVWTAQTRPNMNLDDPHVDLPRLVHLIEDSFNRKLDVKDYLNRVNGRLAGLIIAGEYEGGAILTWELPPGVEDDGSESSTARMVPYLDKFAVLKRSQGAGGVADIVFNAMVRTCFPNGVCWRSRKDNPVNKWYFERSLGSWKLADTNWTMFWTG"
  },
  {
    "reference": "MKKTKRIFTALSHLFSPKWWKKNWQRVVLLFFFAVFALLLIFRFVPIPFSAYMVQQKIANLLQGDFRYQIQYDWVSLENISPNIQLAVISSEDQRFPEHLGFDFEAIQRAIRYNEKSNKGIRGASTISQQTAKNLMLWHGQNWLRKGLEVPATMLLELTWSKKRILEVYLNIAEFGNGIFGVEAASRYYFKKSAKNLSQNEAALLVAVLPNPIIYKVNKPSLLVRKKTSLDFETNGKFRY",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that contributes to peptidoglycan biosynthetic process, cell wall organization by functioning in plasma membrane, peptidoglycan-based cell wall with pentosyltransferase activity, peptidoglycan glycosyltransferase activity.2. A protein that is capable of catalyzing reactions within the Cell wall biogenesis pathway with high efficiency and specificity.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "LEPGMSYNKQEMVALLEGTQYRQVSRMTRPGEFTVQANSIEMIRRPFDFPDSKEGQIRARLSFSNGELSEIKNLDSGRDFGFFRR",
    "response#2": "LQRDWRSWEQLPDSLKVAVIAGEDQHFAEHHGFDLPAIQQALAHNERGGSIRGASTLSQQVAKNVFLWSGRSWLRKGLEAWFTLLIETLWTKQRILEVYLNSAEWGPGVFGAQAAARYHFGLDAERLSRTQASQLAAVLPSPLKWSAARPGPYVRQRAAWIRQQLA",
    "response#3": "AMVIVDRFSGEVRAMVGGADPQFAGYNRALQARRSIGSLAKPATYLTALGQPDKYRLNTWIADNPIALKQPNGQIWKPQNDDRQFSGQVMLVDALTRSMNVPTVNLGMTLGLPQVVDTWTKLGVPKEQLNSVPAMLLGALNLTPVEVAQAFQTIASGGNRAPLSALRSVIAEDGTVLYQSFPQAERAEPAQAAYLTLYGMQQVAERGTARALGALFPNAHLAAKTGTTNNLVDSWFAGIDGL"
  },
  {
    "reference": "MNDLNKEEPQEIEAFSDRLISTHEAIKMFQEGQPIILVDDEDRENEGDIAIPAQFVTEELVNFMVSEARGLVCVPMTEERADHLALPLMTDHGNAPLGTAFTISVEAASGVTTGISAPDRARTINVLADIKSISTDFTRPGHIFPLRAKEGGVLVRAGQTEASVDLCRLAGVTPVASICEIMNEDGTMARMPDLIKFADKHDLKIATVADLIRYRMQTERLIHRKGETNLPTLYGEFKAIGFSTGIDSKEHVALVFGDINPEEPTLVRVHDKCLTGDVFGSLRCDCGEQLQAALRMIADEGSGVVVYMDQEGRGIGLHNKLRAYNLQDDGLDTVEANEALGLPAESRDYGIGAQILVDLGVRKMRLMTNNPIKIQELNTMGVLRGAGLDGFGLQVTERIPIEIEPNEHNVRYLTAKRDKMGHMFEMPMPDFG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein have GTP cyclohydrolase II domains with specific sequence motifs that are crucial for its activity.2. A protein with GTP binding, manganese ion binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity functions is required to enable riboflavin biosynthetic process.3. The protein should be able to bind Mg(2+), Zn(2+) ligand in a variety of conditions4. The designed protein must have a Nucleophile; for GTP cyclohydrolase activity that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "GVDMPTQWGDFSLIAYTQVATGDTHLALVKGEWAPGEPVLTRVHSSCVTGDIFGSCRCDCGGQLHRAMTMVEEAGKGVVLYMFQEGRGIGLLNKLKAYKLQEMGRDTVEANLELGFKMDERDYGVGAQILRDLGVSKLRLMSNNPKKRNALAGYGLEITETVI",
    "response#2": "GVDMPTQWGDFSLIAYTQVATGDTHLALVKGEWAPGEPVLTRVHSSCVTGDIFGSCRCDCGGQLHRAMTMVEEAGKGVVLYMFQEGRGIGLLNKLKAYKLQEMGRDTVEANLELGFKMDERDYGVGAQILRDLGVSKLRLMSNNPKKRNALAGYGLEITETVK",
    "response#3": "RTAEVHLPTRFGDFECYLYESIFEEPAHLALTMGGVGKRNESGISPESKETVLVRVHDECLTGDVFHSMRCDCGQQLEAAMAMIAGEGSGVLLYMRQEGRGIGLENKLKAYELQENGMDTVEANEELGFPADLREYGIGAQILADLGIRKIRLLTNNPKKIVGLEGYGLEIVDRMGG"
  },
  {
    "reference": "MHLKYSQFEKLAEEKKISDVIIYNQRFEVQGSIGKRPFDVNYGDIEQPIKILKANGIKYDFDTSAPSINWTDVFPYLLLGGILIFLFMFMLQQVQGGGNKVMSFGKAKAKKLSQDHPKTTFKDVAGVDEAVEELEEIKDFLSSPGKFLALGAKIPKGVLLYGPPGSGKTLLARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKMHAPSIVFMDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFDIKDNVILIAATNRPDILDPALLRPGRFDRQIVVDRPDLKGRIKILDVHSRNKPLAKEVDLEVLARRTPGFTGADLANLMNESALLTARHRKKKIDMKELEEAIDRVVAGPEKKTRLISDKEKEVIAYHESGHALVAHTLPNTDPVHKISIIPRGRSLGYTLTLPAEDRYLISKSELLDQLAMLLGGRVAEEIIFHDATTGAQDDLDRATKLARKMVCEYGMSEQLGPLTLGQKQDQVFLGRDLASNPDYSQEIAYEIDKEIRRIVDEGWDKAKQILQENKVQLDTIAKSLMEFETIEKEELINLLNGEPIERFREEKEASLKKPVKKKLGARVPGKLRPAES",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.2. The protein should be able to bind ATP ligand in a variety of conditions3. The protein should have a Helical domain that is long enough to span the entire membrane.4. A protein with metalloendopeptidase activity, ATP binding is needed to enhance protein catabolic process in a specific context.5. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins The designed protein sequence is ",
    "response#1": "VLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFENAKKTAPSIIFIDEIDAVGRQRGAGLGGGNDEREQTLNQLLVEMDGFQDDGNSVIVIAATNRSDVLDPALLRPGRFDRKVLVL",
    "response#2": "IPKGVLLMGPPGTGKTLLARAIAGEAGVPFFTISGSDFVEMFVGVGASRVRDLFLQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFESNEGVILISATNRPDVLDPALLRPGRFDRQVVVSLPSREELIRRIIP",
    "response#3": "VLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFENAKKTAPSIIFIDEIDAVGRQRGAGLGGGNDEREQTLNQLLVEMDGFQDDGNSVIVIAATNRSDVLDPALLRPGRFDRKVLVV"
  },
  {
    "reference": "MTKKSTKAPAKKAAPAAKAAPAAKRGAAADSGKVAKTQLVEMVADQTGLTKKQSEEAISSMLECVVSALQSGKSVGLPGLGTLSVKETAARTGVRPGTSEKIQIPAGKKVAFKVASTLKGNL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that localizes to cytosol and can perform structural constituent of chromatin with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "LKPETPKGPIIISKNPSNGIFYGPSFTKRESLNARMFLKYYGAHKFLDTYLPEDLNSLYIYYLIKLLGFEVKDQALIGTINSIVHINSQERVQDLGSAISVTNVEDPLAKKQTVRLIKDLQRAINKVLCTRLRLSNFFTIDHFIQKLHTARKILVLL",
    "response#2": "LKPETPKGPIIISKNPSNGIFYGPSFTKRESLNARMFLKYYGAHKFLDTYLPEDLNSLYIYYLIKLLGFEVKDQALIGTINSIVHINSQERVQDLGSAISVTNVEDPLAKKQTVRLIKDLQRAINKVLCTRLRLSNFFTIDHFIQKLHTARKILVLC",
    "response#3": "LKPETPKGPIIISKNPSNGIFYGPSFTKRESLNARMFLKYYGAHKFLDTYLPEDLNSLYIYYLIKLLGFEVKDQALIGTINSIVHINSQERVQDLGSAISVTNVEDPLAKKQTVRLIKDLQRAINKVLCTRLRLSNFFTIDHFIQKLHTARKILVLA"
  },
  {
    "reference": "MASARRRRIKKKKQVISKKIEFRYRGYTLEELQQMPLREFAKLLPARQRRTLLRGLTPQQKKLAMKIKKARRLLNKGKEPRIIRTHCRDFVITPDMVGLTFGVYNGKEFVEVKVTPEMIGHYLGEFSLTRKPVQHGAPGMGATRSSMFVPIK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design must be able to perform rRNA binding, structural constituent of ribosome in order to contribute to translation, ribosomal small subunit assembly. The designed protein sequence is ",
    "response#1": "MPKFEVRGAFKTLEGWQKFTKVVEANNERYALEKVYSLIGSNHKVKRNLIKIEEVR",
    "response#2": "KYIDLMSIYLPHTGGRHEHRRFGKSRIPIVERLINNLMRPGRNKGKKMLAYNIVKTTFDIIAVKTGQNPIQVLVRAIENAAPREEVTRIMYGGIVYYVAVDVAPQRRVDLALRHLVTGASEASFNNPKPIEEALAEEIIAAANNDNKSVAIRKKEEIERIALSSS",
    "response#3": "EIANAMAKGVRFRRVMFVAIRQLMEAGAKGFEIVVSGKLSTERARFEKQTYGKLYKIGYDAKNRVRRAVVHVLLKPGIYGIEVRL"
  },
  {
    "reference": "MAKLETVTLGNIGKDGKQTLVLNPRGVNPTNGVASLSQAGAVPALEKRVTVSVSQPSRNRKNYKVQVKIQNPTACTANGSCDPSVTRQAYADVTFSFTQYSTDEERAFVRTELAALLASPLLIDAIDQLNPAYWTLLIAGGGSGSKPDPVIPDPPIDPPPGTGKYTCPFAIWSLEEVYEPPTKNRPWPIYNAVELQPREFDVALKDLLGNTKWRDWDSRLSYTTVRGCRGNGYIDLDATYLATDQAMRDQKYDIREGKKPGAFGNIERFIYLKSINAYCSLSDIAAYHADGVIVGFWRDPSSGGAIPFDFTKFDKTKCPIQAVIVVPRA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Minor capsid protein..2. The designed protein should have a specific function within the viral capsid.3. The intended protein structure should align with strand-strand-strand-strand-helix-strand-turn-strand-helix-helix-strand-strand-strand-helix-strand criteria. The designed protein sequence is ",
    "response#1": "YFGEISIGTPPQNFLVLFDTGSSNLWVPSVYCQSQACTSHSRFNPSESSTYSTNGQTFSLQYGSGSLTGFFGYDTLTVQSIQVPNQEFGLSENEPGTNFVYAQFDGIMGLAYPALSVDEATTAMQGMVQEGALTSPVFSVYLSNQQGSSGGAVVFGGVDSSLYTGQIYWAPVTQELYWQIGIEEFLIGGQASGWCSEGCQAIVDTGTSLLTVPQQYMSALLQATGAQEDEYGQFLVNCNSIQNLPSLTFIINGVEFPLPPSSYILSNNGYCTVGVEPTYLSSQNGQPLWILGDVFLRSYYSVYDLGNNRVGFV",
    "response#2": "QFPVLGRTQAAYLAPGENLDDKRKDIKHTEKVITIDGLLTADVLIYDIEDAMNHYDVRSEYTSQLGESLAMAADGAVLAEIAGLCNVESKYNENIEGLGTATVIETTQNKAALTDQVALGKEIIAALTKARAALTKNYVPAADRVFYCDPDSYSAILAALMPNAANYAALIDPEKGSIRNVMGFEVVEVPHLTAGGAGTAREGTTGQKHVFPANKGEGNVKVAKDNVIGLFMHRSAVGTVKLRDLALERARRANFQADQIIAKYAMGHGGLRPEAAGAVGLAVTNPTIRCVFSTSATEYEMQLLDGSTVVKFLKVKYGFNTGDTLVIDCHERSVTLNGQDIMPALLIQSDWIQLKPQVNTYLKATQPSTIVFTEKR",
    "response#3": "MINQEPYPMIVGEPSLIGQFIQELKYPLAYDFVWLTKSNPHKRDPMKNATIYAHSFLDSSIPMITTGKGENSIVVSRSGAYSEMTYRNGYEEAIRLQTMAQYDGYAKCSTIGNFNLTPGVKIIFNDSKNQFKTEFYVDEVIHELSNNNSVTHLYMFTNATKLETIDPVKVKNA"
  },
  {
    "reference": "MGNQSLVVLTESKGEYENETELPVKKSSRDNNIGESLTATAFTQSEDEMVDSNQKWQNPNYFKYAWQEYLFIFTCMISQLLNQAGTTQTLSIMNILSDSFGSEGNSKSWLMASFPLVSGSFILISGRLGDIYGLKKMLLVGYVLVIIWSLICGITKYSGSDTFFIISRAFQGLGIAFVLPNVLGIIGNIYVGGTFRKNIVISFVGAMAPIGATLGCLFAGLIGTEDPKQWPWAFYAYSIAAFINFVLSIYAIPSTIPTNIHHFSMDWIGSVLGVIGLILLNFVWNQAPISGWNQAYIIVILIISVIFLVVFIIYEIRFAKTPLLPRAVIKDRHMIQIMLALFFGWGSFGIFTFYYFQFQLNIRQYTALWAGGTYFMFLIWGIIAALLVGFTIKNVSPSVFLFFSMVAFNVGSIMASVTPVHETYFRTQLGTMIILSFGMDLSFPASSIIFSDNLPMEYQGMAGSLVNTVVNYSMSLCLGMGATVETQVNSDGKHLLKGYRGAQYLGIGLASLACMISGLYMVESFIKGRRARAAAEYDCTVA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The Extracellular, Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.2. The ideal protein would be one that plays a critical role in borate transport through borate efflux transmembrane transporter activity within membrane, plasma membrane, vacuole, cell periphery.3. The protein must exhibit the following characteristics: Probable ATP-dependent export permease; Appears to confer resistance only to aminotriazole.; Putative component of the machinery responsible for pumping aminotriazole (and possibly other toxic compounds) out of the cell.4. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "LINTMIYAGIAAPLTALFGLLIAYIVVRKDFQGKKTLEFLTMLCFAVPGTVAGVSYILAFNDAPMYITGTGIIIIISMVMRDLPIGMRAAIAGLGQLDKSLDEASLSLKGSSLKTIWFIVFPLLKPALLSALVTSFVRAMTTVSAIVFLVTADTRVATAYILNRVEDGEYGVAIAYGSILIVVMMAIILFFAG",
    "response#2": "FGSEIVNDVKKRLPYYKSDWIDACHYRVLPACLNIYFSNLLPELAFALDMFAKTNNSFGVNEVLLASVLGSVVFALLSSQPLCIVGVTGPITVFNYTVYDIMHDRGTPYFPFLCWICLWSMIFHFIIAIANGVYFVKHITKFSCEIFGLYVAFIYLEKGVQVLCH",
    "response#3": "LKAVNFTAKPGEVTAIIGPSGAGKTTILRSINQLIRDDSGQILLDDTDIRQANKAELRKVRHHIGMIFQNYNLISPLTALENVLHGRLGAKSTVAGMLGLYSSAEKQEALQLLDEVGLKEYAYQRCDQLSGGQQQRVGIARALMQHPKMILCDEPIG"
  },
  {
    "reference": "MFRTISNIFRVGDLRRKVIFTLLMLIVFRIGSFIPVPGTNREVLDFVDQANAFGFLNTFGGGALGNFSIFAMGIMPYITASIVMQLLQMDVVPKFAEWAKEGEAGRRKLAQFTRYGTIVLGFIQALGMSVGFNNFFPGLIPNPSVSVYLFIALVLTAGTAFLMWLGEQITAKGVGNGISIIIFAGIAAGIPNGLNLIYSTRIQDAGEQLFLNIVVILLLALAILAIIVGVIFVQQALRKIPVQYAKRLVGRNPVGGQSTHLPLKVNAAGVIPVIFALSLFIFPPTVAGLFGSDHPVAAWVIETFDYTHPIGMAVYALLIIGFTYFYTFIQVNPERMAENLKKQGGYIPGIRPGKATQTYITRILYRLTFVGSLFLAVVAILPVFFIKFADLPQAIQIGGTGLLIVVGVALDTMKQIEAQLIKRSYKGFIK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in plasma membrane that is critical for intracellular protein transmembrane transport, protein transport by the Sec complex.2. For general function, the protein need meet that The ring and the pore may move independently.; These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back The designed protein sequence is ",
    "response#1": "LDDNGRRRLADLTAQGNENSFAIVVDNQVVSAPTVSGPLDTSELQIEGAFDLQAANNMAVVLRSGALPQAVTVLEERTIASALGEDYASAAVLAALLAALVVGLFMVLSYGILGVIALVALVVNIVILTAVLSLIGASISLASIAGLVLTIGLAVDAHILIYERVREDRRKGYSVVQAMESGFYRALSTIVDANLTTLIAALVLFLLGSGTVHGFALTVAIGIGTTLFTTL",
    "response#2": "LDDNGRRRLADLTAQGNENSFAIVVDNQVVSAPTVSGPLDTSELQIEGAFDLQAANNMAVVLRSGALPQAVTVLEERTIASALGEDYASAAVLAALLAALVVGLFMVLSYGILGVIALVALVVNIVILTAVLSLIGASISLASIAGLVLTIGLAVDAHILIYERVREDRRKGYSVVQAMESGFYRALSTIVDANLTTLIAALVLFLLGSGTVHGFALTVAIGIGTTLFTTLF",
    "response#3": "LDDNGRRRLADLTAQGNENSFAIVVDNQVVSAPTVSGPLDTSELQIEGAFDLQAANNMAVVLRSGALPQAVTVLEERTIASALGEDYASAAVLAALLAALVVGLFMVLSYGILGVIALVALVVNIVILTAVLSLIGASISLASIAGLVLTIGLAVDAHILIYERVREDRRKGYSVVQAMESGFYRALSTIVDANLTTLIAALVLFLLGSGTVHGFALTVAIGIGTTLFTTL"
  },
  {
    "reference": "MITIKKGLDLPIAGTPAQVIHNGNTVNEVAMLGEEYVGMRPSMKVREGDVVKKGQVLFEDKKNPGVVFTAPASGTVVTINRGEKRVLQSVVIKVEGDEQITFTRYEAAQLASLSAEQVKQNLIESGLWTAFRTRPFSKVPALDAIPSSIFVNAMDTNPLAADPEVVLKEYETDFKDGLTVLTRLFNGQKPVYLCKDADSNIPLSPAIEGITIKSFSGVHPAGLVGTHIHFVDPVGATKQVWHLNYQDVIAIGKLFTTGELFTDRIISLAGPQVKNPRLVRTRLGANLSQLTANELNAGENRVISGSVLSGATAAGPVDYLGRYALQVSVLAEGREKELFGWIMPGSDKFSITRTVLGHFGKKLFNFTTAVHGGERAMVPIGAYERVMPLDIIPTLLLRDLAAGDTDSAQNLGCLELDEEDLALCTYVCPGKNNYGPMLRAALEKIEKEG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm; NqrA to NqrE are probably involved in the second step, the conversion of ubisemiquinone to ubiquinol.2. The protein should have a fast turnover rate for a ubiquinone + H(+) + n Na(+)(in) + NADH = a ubiquinol + n   Na(+)(out) + NAD(+).3. A protein with oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor is needed to enhance sodium ion transport in a specific context. The designed protein sequence is ",
    "response#1": "GLWSMMYAFVAIDTDGKTVRGITYYDHGETPGLGGEIENPIWRNQWIGKRLFDDQGQPAIRIVKGRAPANDPHAVDGLSGATLTSNGVQNSFNFWLGEGLWSMMYAFVAIDTDGKTVRGITYYDHGETPGLGGEIENPIWRNQWIGKRLFDDQGQPAIRIVKGRAPANDPHAVDGLSGATLTSNGVQNSFNFWQ",
    "response#2": "GLWSMMYAFVAIDTDGKTVRGITYYDHGETPGLGGEIENPIWRNQWIGKRLFDDQGQPAIRIVKGRAPANDPHAVDGLSGATLTSNGVQNSFNFWLGEGLWSMMYAFVAIDTDGKTVRGITYYDHGETPGLGGEIENPIWRNQWIGKRLFDDQGQPAIRIVKGRAPANDPHAVDGLSGATLTSNGVQNSFNFWN",
    "response#3": "GLWSVMYGFVAIQPDGNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHGVDALSGASLTSKGVQGSFAYWFGEGLWSMMYAFVAIDTDGKTVRGITYYDHGETPGLGGEIENPIWRNQWIGKRLFDDQGQPAIRIVKGRAPANDPHAVDGLSGATLTSNGVQNSFNFWLGELAGDA"
  },
  {
    "reference": "MALNLCTSPKWIGTTVFDSASSSKPSLASSFSTTSFSSSILCSKRVGLQRLSLRRSISVSVRSSLETAGPTVTVGKVTEVNKDTFWPIVNAAGDKTVVLDMFTKWCGPCKVIAPLYEELSQKYLDVVFLKLDCNQDNKSLAKELGIKVVPTFKILKDNKIVKEVTGAKFDDLVAAIDTVRSS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein localizes to the chloroplast.2. The designed protein must have a Nucleophile active site that is highly conserved among related enzymes.3. The protein must exhibit the following characteristics: Participates in various redox reactions through the reversible oxidation of the active center dithiol to a disulfide; The F form is known to activate a number of enzymes of the photosynthetic carbon cycle..4. The designed protein must have at least one Thioredoxin domain. The designed protein sequence is ",
    "response#1": "RIYLLTFEGHLNVHFQNYNGKKSSSVYSISLWGHEGLKPINKNLSLFTLLPIKNNESFYKNPHGDIKKTIQSFLITNNCDNKKIVPYPHESGNTMLFPLLVLIIFTGVIGFIGIPFDQENMDFDILSSWLTPSVNLLHLKLNNKNSFDWSEFLTNATLSVSIAYSGIVLASFLYKPIYSYSSLQNFALINLFAKRHPKRFFSDKIKNVIYDWAHHRGYIDAFYTRYIIRSVRGLSQFINFFG",
    "response#2": "MAEEAQVIACHTVQEWNEKFQKAKDSGKLIVIDFTASWCGPCRVITPYVSELAKKFPHVAFFKVDVDDLKDVAEEYKVEAMPSFVILKEGQEVERIVGARKDELLHKIAVHAPIS",
    "response#3": "GNLFVFDSRLSLPPAAYNDNVVDKAVGDNVVDEAGRTPQTPVDTSFARCYLCNSQVQELRHRNCANLDCNRLFLCCAECVVDLKGCCCSDR"
  },
  {
    "reference": "SLIQFETLIMKVVKKSGMFWYSAYGCYCGWGGHGRPQDATDRCCFVHDCCYGKVTGCDPKMDSYTYSEENGDIVCGGDDPCKREICECDRVAAVCFRDNLDTYNSDTYWRYPTKNCQEEPDPC",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be involved in arachidonic acid secretion, phospholipid metabolic process, lipid catabolic process by localizing to extracellular region and exhibiting calcium ion binding, toxin activity.2. The protein must exhibit the following characteristics: Snake venom phospholipase A2 (PLA2) that inhibits ADP-induced platelet aggregation; PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides..3. The stability of the protein-Ca(2+) complex should be optimized to maximize enzymatic activity.4. A key design requirement for the protein is the optimization of the active site to achieve high catalytic efficiency for a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-   glycero-3-phosphocholine + a fatty acid + H(+).5. The designed protein should have a high specificity for Ca(2+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "LMQFENMIKKVTGRSGIWWYGSYGCYCGKGGQGLPQDASDRCCFVHDCCYGKVNGCDPKDDFYVYSSENRDIVCGEDNPCTKEICECDKAAAICFRDNMDTYQNKYWFYPSSNYC",
    "response#2": "LIQFSQLIQCANKGKRATYHYMDYGCYCSKGGSGTPVDALDRCCKTHDDCYGQAEKKGCFPLLSLYNFACFPGAPQCGKGNTCQRFVCACDLKAALCFAKSPYNNNC",
    "response#3": "DLMQFETLIMKSGVWYYGSYGCYCGSGGQFRPQDASDRCCFVHDCCYGKNGDIVCGGDDPCKKQICECDRVAATCFRDNKVTYDNKYWFFPAKFSSFLPPPTL"
  },
  {
    "reference": "MHCQLGGQARALIFLMFESCRNSWHVSPWSSVPFLPSPCLFFSFSALPFLHPLSQLIACRGSSATWLFPVSSGTLVLRYALAASPPRGTMRPSTNPRTAEEGRPWLIHATQTGPQTASVLSLFPAVNAGFFSACRQHRGGRPPCLLNHRRLLLGLVSVLTVFLSCLPFTNATVSPAALQDVCYAFGNISRKLSSFLVPSRVTCPRGATPLSGVEAQDSLEHPEIPDFRFLVYDVKNGEGFHLQKEVIYRVALVISLLNARSAQQGRMTDVHTAEKAREDRGHPQASHMLCASSSFSHACSARSTFPFFPMWVLVLPPWCRLAHWHFSEETITAMAENSWLKHVRWGTFFDFQDLGERLPVMEYEDFLTYQLMRPDPWGERRQRKEKQTTPVELDVVLSVRFSSTPSSRSLPFCACLSSRASEIADEQAQRDDVCCNVNDLPGCPQIHAALTPQERQRAPAQRGGDPREEIDEQTGEFNEGHNPSGDGEREKRKPGRRSDTSRSRKEIQEEAKVSDTWSGVSLWLAGFCETVRALEMWCASLYIADAPRIADLLWRSVAERPPGAIQTVWLKFGENLLVPWPDVLLDAHLLDMLHVHPKLRQIGDLFINKFLSNRDKTETGKGERASTEGGTEGDENLAKHGYIAAHLRRTDFLYLKRSVPLQRAAAYLVSRMKEHGVFKAFICTDGSEDEKRELRDAVRRVGDAASSSPYTVVFFDLPTVRRLMIKTLEASSHVSDDGSFHDLKAVETPGYSGPNHISLLLHPGITAL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: O-fucosylates microneme protein MIC2 and may play a role in its stabilization; Probably by regulating protein O- fucosylation, may play a role in tachyzoite adhesion to and/or invasion of host cells; however, POFUT2 involvement in adhesion/invasion is controversial..2. The protein should contain Helical; Signal-anchor for type II membrane protein transmembrane regions to facilitate transport across the membrane.3. The designed protein should be able to compete with endogenous proteins or inhibitors for GDP-beta-L-fucose binding.4. The designed protein should have peptide-O-fucosyltransferase activity within endoplasmic reticulum, endoplasmic reticulum membrane to regulate fucose metabolic process, protein O-linked fucosylation.5. We require a protein that can specifically bind to the metabolites produced in the protein glycosylation, Protein modification pathway.6. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for GDP-beta-L-fucose + L-threonyl-[protein] = 3-O-(alpha-L-   fucosyl)-L-threonyl-[protein] + GDP + H(+).7. The Lumenal, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "LRYVSLFLNSSTLGFLFLITRRINCSRLISATGLLILSNWETFRNEGTIISFDSLEWCLFSVVIYSFISISIAKLGTTNWFANVITLSISLGLAISSKFIGIVTWAFVILSFVRQFDRLISDVKVTTIQIIKFVILCLLFVLIIPGSIFLLMFNSHLLLVMLSLEFLVLGLFCLVMFITSSNLEPERVSNYFLLIYVVTATALPLLLALSVYMYQEMFVLYMQSHCDVPSDRDRYVKELMKHLEIDSYGQCMKNREHPNKRLEDTSTATTEDPEFMAFTARYKFHLAMENAICADYMTEKLWRPMHLGAIPIYRGSPSVRDWMPNNHSIIMIDDFASPKELAEFIMTLDSDDEQYLKYLEYKKPGGTTNTFLPLAYVQSDCDPPSDRDSYVQELMKYIAVDSYGECLHNRDLPQQVNNPSSMDDSQFHHILAQYKFILAFENAVCEDYITEKLWRPLKLGAVPVYFGAPNVENWLPSNKSAIIVGRFSHPKELATYIKKLDKNDKLYMQFIEWKLHGNINNKHLLSTITERKWGVQDVTQDNYIDAFECMICKRVWEQNDTRESGVWYTSKNDSVYAIVLDWPNSGQLTLGVPRSSPTTTVTMLGWAAPLKWVAVSGSGITVQMPTASSNQLPCQWAWVIRLF",
    "response#2": "LRYVSLFLNSSTLGFLFLITRRINCSRLISATGLLILSNWETFRNEGTIISFDSLEWCLFSVVIYSFISISIAKLGTTNWFANVITLSISLGLAISSKFIGIVTWAFVILSFVRQFDRLISDVKVTTIQIIKFVILCLLFVLIIPGSIFSSLGAGPWWIRIAAVVAAYWTISISLVFLNKYLLSSPDIKLDAPLFVTWYQCVVTVICLFFLSLLGDRYPWIDTFPAFHIKLSVAKQVLPLSAVFVGMITFNNLCLKNLGVSFYNVGRSLTTVFNVICTYVILGQSTSYKAVICCAVIIGGFLMGVDQEGSSGKISYSGVLFGVLASLCVSLNAIYTKKFIPAVDNNIWRLQLYNNFNACFLFLPLMALLGEIGEVAHFPNLSSAYFWLMMTIGGVFGIAIGYITGLQIKVTSPLTHNISGTAKACVQTIMSVSYFHETKTALWWLSNAMVLGGSMALYADTYNTCLSKHSPLAYVQSDCDPPSDRDSYVQELMKYIAVDSYGECLHNRDLPQQVNNPSSMDDSQFHHILAQYKFILAFENAVCEDYITEKLWRPLKLGAVPVYFGAPNVENWLPSNKSAIIVGRFSHPKELATYIKKLDKNDKLYMQFIEWKLHGNINNKHLLSTITERKWGVQDVTQDNYIDAFECMICKRVWELPGLKFGSPSKEKLVCWVVSNWNPEHARVKYYNELSKSIEIHTYGQAFGEYVNDKNLIPTISTCKFYLSFENSIHKDYITEKLYNAFLAGSVPVVLGPSRENYENYIPADSFIHVEDYNSPSELAKYLKEVDKNNKLYLSYFNWRKDFTVNLPRFWESHACLACDHVKRHQEYKSVGNLEK",
    "response#3": "LRYVSLFLNSSTLGFLFLITRRINCSRLISATGLLILSNWETFRNEGTIISFDSLEWCLFSVVIYSFISISIAKLGTTNWFANVITLSISLGLAISSKFIGIVTWAFVILSFVRQFDRLISDVKVTTIQIIKFVILCLLFVLIIPGSIFAWGEIRNVGTGLCTDTKHGTLGSPLRLETCIRGRGEAAWNSMQVFTFTWREDIRPGDPQHTKKFCFDAVSHTSPVTLYDCHSMKGNQLWKYRKDKTLYHPVSGSCMDCSESDHRIFMNTCNPSSLTQQWGEIRNVGTGLCTDTKHGTLGSPLRLETCIRGRGEAAWNSMQVFTFTWREDIRPGDPQHTKKFCFDAVSHTSPVTLYDCHSMKGNQLWKYRKDKTLYHPVSGSCMDCSESDHRIFMNTCNPSSLTQQWLFN"
  },
  {
    "reference": "MHMKVAVVGATGLVGTQMLKVLAERNFPVTELLPVASARSVGQEIEFRGQKYRVVSMDDAIAARPAVAIFSAGGAISLQEAPRFAAVGTVVIDNSSAWRMDPAKKLVVPEINAHTLTADDKIIANPNCSTIQLVVALNELHKRYQALRIVVSTYQSVTGTGKQAVDQLLQERAGQTPANPAYPHRIDLNVLPHIDVFQDNGYTKEEMKMVNETKKIFGDDTIRLTATTVRVPVMGGHSEAVNVEFAQEFDLADVRELLRQTEGVELVDDVQNNIYPMPQDAHGRDAVLVGRLRRDETQPRTLNMWVVADNLRKGAATNAVQIAEAMLKMGLLG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that is capable of catalyzing reactions within the Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5, L-threonine biosynthesis pathway with high efficiency and specificity.2. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.3. The design of the protein must focus on the precise arrangement of amino acids in the Acyl-thioester intermediate, Proton acceptor to enable effective catalysis of L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.4. The protein design must be able to perform NADP binding, NAD binding in order to contribute to threonine biosynthetic process, diaminopimelate biosynthetic process, lysine biosynthetic process via diaminopimelate, 'de novo' L-methionine biosynthetic process, isoleucine biosynthetic process.5. The protein should be able to bind phosphate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MAIVGATGAVGQEMMKVLAERDFKIDELICLADPREEGTQLKFKDEVITVQKVTEEAFRKADIALFAVGTEISKELAPIAVKNNCVVIDNSYAFRLEEGVPLVVPEVNAEDIKDAAKPKKGIIANPNCTTMAAMPVLKPLHDKAKILRVVVSTYQSVSGAGKQAMDELFAQTRAIFVSDSVETKNFPKRIAFNVIPEIDVFMEDGYTKEEWKMMAETKKILDPKIKLTATCVRVPVFIGHSEAVHIEFENPISADEARDILREAPGCLVIDKRERGGYITPHEAAGEDATYISRIREDATVENGLVLWCVADNLRKGAALNAVQIAELVAAA",
    "response#2": "MAIVGATGAVGQEMMKVLAERDFKIDELICLADPREEGTQLKFKDEVITVQKVTEEAFRKADIALFAVGTEISKELAPIAVKNNCVVIDNSYAFRLEEGVPLVVPEVNAEDIKDGALHRGVKSNIIANPNCTTIVAMLGGLFEQGLVEWASAMTYQAASGAGAQNMRELINQMGAIQQSVATELSDPSSAILEIDRKVVEAMRSDSFPVDQFGVPLAGSLIPWIDKQLTNGQSREEWKAQAETNKIIGRSGRPIPVDGICVRIGAMRSHSQALTIKLNKDVPIADIESILDEHNAWSKVVPNEKEVTMTDLTPTAVTGTLTVPVGRLRKLNMGSQYLSAFTVGDQLLKGAALNAVQIAEKLIEMK",
    "response#3": "MAIVGATGAVGQEMMKVLAERDFKIDELICLADPREEGTQLKFKDEVITVQKVTEEAFRKADIALFAVGTEISKELAPIAVKNNCVVIDNSYAFRLEEGVPLVVPEVNAEDIKDGVAKGVKNIVANPNCTTMAAMPVLAPLAARRAMRRVVVSTYQSASGAGARAMEELSELSRTVLDGGTPMSSVLPYSLAFNLFLHNSPLQGNGYCEEELKMVNETRKIMGLPDLRVSATCVRVPVLRAHSEAINIEFCEPFPVDEARALLQDAPGVELIEDFSSNRFPMPTDVTGRDPVAVGRIRQDLSEPNALELWLCGDQIRKGAALNAVQIAELVAGR"
  },
  {
    "reference": "MYKPISLFIALRYLWNTHLPNFKKIITILSIIGISITTASLIIITSIINGSEKNFEKNILSFIPHLIITNKNQCIKKDQFPENILKLNNIKNISDLISKEIIVQSKNDISMAEVIGIDHTNYYNIHNYNIKSVLKTLKPGYYNIIIGKQLARKLNVFIGDRLKLIFLSNTKNFFSGEIFKQRTFKIINFFSTKKEVDYYQILMNKEDSLNFLNYSKDYVTGWRVWLKNPLSLNVNEIKKITHPLFLLDWTTQKGELFKAMKIEKYIMFLFLFLVLLVSILNIVVILTICTVEKQNAVAILQTQGLLNCKIMLIFIIFGSSTAIIGNILGTLISLTLIIQNDFLKFFINIFIDETNIPIIVIPYQIFFINITITLFTILSTLYPSWKAIQLKPSRILSNE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should be able to enhance the efficiency of lipoprotein transport. The designed protein sequence is ",
    "response#1": "LGLAQSLALTLLLTGSFKCFIGGLRPNFLEVCDPTPASIAAGNPPVGFSKIYYDRSICSADDSIVNDALSAYPSGHSSITAASFGFLALFIHAKFKIFDNRGHIFLYVIVSGCIIGAGLIGISRVADYRHTFLNVLAGWSIGLIIALSCYRLY",
    "response#2": "LGLAQSLALTLLLTGSFKCFIGGLRPNFLEVCDPTPASIAAGNPPVGFSKIYYDRSICSADDSIVNDALSAYPSGHSSITAASFGFLALFIHAKFKIFDNRGHIFLYVIVSGCIIGAGLIGISRVADYRHTFLNVLAGWSIGLIIALSCYRLY",
    "response#3": "GKWYEIEKIPVSFEKGSCIQANYSLKENGNVEVINKELRADGTVNQIEGEATPENITEPAKLAVKFFWFMPSAPYWVLATDYENYALVYSCTTIIWLFHMDHVWILGRNPYLPPETVTYLKDILTSNNIEVEKMTITDC"
  },
  {
    "reference": "MTTMELSAFVDRLRSGASPKFPQDANSLDFARNLDSQDKLSHLRDEFVLPTKGSLKKTALDGSIPSNGAVQNGSNGTNGHSNGNKASSHDSDQCIYFVGNSLGAQPKAVRQYLNAQLETWASIGVNGHFTSMDNSPLTSWQDLAEDCAIKSADIVGSSPSEIVIMNTLTVNLHLMMASFYKPSEKRHKIILEWKPFPSDHYAIESQVVWHGLDPEKSMVKIHPNEDYLITTDEVLATIDEHGEETALILLPGIQYYSGQLFDIPRITAHAQSKGIVVGWDLAHAAGNVELKLHDWNVDFACWCTYKYINAGPGSIAGAFVHEKHGQVEFKDESGQPTYRPRLMGWYGGDKSVRFNMDNMFVPTRGAGGYQLSNPSAIDLASLSGALSVFNKTSMHDLRSKALVLTAYAEHLLDQMLAESTDETPLFRVITPRDPAQRGTQLSVLLREGLLENVAEALEGNGVMCDKRKPGVIRVAPVPLYSRFEDVWTFMKVLRQSLGL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively..2. The protein design must be able to perform pyridoxal phosphate binding, kynureninase activity in order to contribute to tryptophan catabolic process, quinolinate biosynthetic process, L-kynurenine catabolic process.3. The protein design should emphasize Polar residues compositional bias.4. The protein design should take into account the physiological conditions of the Cofactor biosynthesis, NAD(+) biosynthesis pathway. The designed protein sequence is ",
    "response#1": "YLCGNSLGLQAKNVSEYLEQELQDWSDFGVHGHTKAKRPWLTYHLQAREGFASLTGSKESEVIAMNTLTVNLHMLMASFYRPTQKRFKILIESTAFPSDRFAAESQIRLHGFDPEDALIEWEPKDDELLLEDYKKIIAEQGDEIALILIPGIQYYSGQVMPMEDLCSIAKKSNIKIGLDLAHAVGNVELELNKWEPDFAAWCTYKYLNGGPGSIGGAFIHEGHQWIEHKTGKKLYNMDNKLIPKNGYKPFSGDRWGSGPMRFVVSKDAYLPFKEVPNGKELDAIRFDEVNGSYAIQFKDGKTWVKDVEESMDAPFCNLDELRKAWATGTPVDESGIAK",
    "response#2": "DHLRTEDIVSKLAEIGDEVAVVWIGCVQYYTGQFFEVEPIARKTHEIGAYFGLDLAHAIGNVPVKLNDWGVDFAAWCTYKYLCGGPGSVAGYYIKDGLDDGGRRLAGWWGHNRQTRFHMSPEFDPIPGAGGYQHSNPPVFSSLPLIATLEIIDKAGGIDVLREKSERLTGYFWDLLTSSKWYRGIASPDRQERGSAA",
    "response#3": "DHLRTEDIVSKLAEIGDEVAVVWIGCVQYYTGQFFEVEPIARKTHEIGAYFGLDLAHAIGNVPVKLNDWGVDFAAWCTYKYLCGGPGSVAGYYIKDGLDDGGRRLAGWWGHNRQTRFHMSPEFDPIPGAGGYQHSNPPVFSSLPLIATLEIIDKAGGIDVLREKSERLTGYFWDLLTSSKWYRGIASPDRQERGG"
  },
  {
    "reference": "MARFIFITGGVVSSLGKGLASAALGALLQARGFKVRLRKLDPYLNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTGVASTRSDNVTTGQIYSKILAKERRGDYLGATVQVIPHVTDAIKEFVRGDLRDEDFVICEIGGTVGDIESLPFLEAIRQLGNELGRARSMFVHLTLVPYITSAGELKTKPTQHSVKELQSVGIQPDMLLCRSEHPIGQDARRKLALFCNLREEAVIQALDVDTIYAVPRAYHEEGMDDVVCRHFGLETPAPDIETWDRITEILRHPDGEVTIGVVGKYTSLIDAYKSLAEALTHGGVANNVKVRINWLDSEIFEKDGHVQELEGVHGILVPGGFGERGSEGKIAAARFARERKVPYFGICFGMQMAVIEAARHLAGMSGAGSSEFGPCDPAVVGLMTEWVKDNAVERRGNADDLGGTMRLGAYPAVLEAGSKVAEIYGQRQISERHRHRYEVNVNLRPALEAVGLHFSGLSPDGELPEIVEVASHPWFIGVQFHPELKSKPFDPHPLFTSFVRAAVEQSRLM",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have metal ion binding, CTP synthase activity, ATP binding to facilitate 'de novo' CTP biosynthetic process.2. The designed protein have Glutamine amidotransferase domains with specific sequence motifs that are crucial for its activity.3. The Nucleophile; for glutamine hydrolysis, active site, Nucleophile of the protein must be engineered to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).4. The protein design should consider the endogenous proteins and enzymes present in the CTP biosynthesis via de novo pathway, Pyrimidine metabolism pathway to avoid interference with native metabolic processes.5. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.6. The designed protein should have a binding site that is specific to the target CTP, UTP, Mg(2+), ATP ligand. The designed protein sequence is ",
    "response#1": "IFVTGGVVSSLGKGIASASLAAILEARGLRVTMMKLDPYINVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFVRTRLTGKNSITTGKIYEAVIRKERRGDYLGATVQVIPHITDEIKHSIFEATRGFDVALVEIGGTVGDIESQPFLEAIRQIRIEHGADKVMFMHLTLVPYIKAAGEIKTKPTQHSVKELRALGIQPDVLLCRCETPLPDSERRKIALFTNVPEKAVISAVDVDTIYAIPRWLHKQGLDELAVAHLKLPDAYLSVVESLNHAGYAIDSEIEIDWVNADEVTADTVVSKLKDADGILVPGGFGDRGLEGKIVAIQYARENNVPFLGICLGMQLACVEYARNVAGLEGAHSTETNPETPYNIIDLMNDQKGVIDLGGTLRLGLYPCHLKAGTVAAKWYDNEEVVQERHRHRYEFNNEYRPLLEEKGLVFSGVSPDNRLVEIVELSDHPFYVACQFHPEFLSRPNRPQKLFLGFMAAAIQVQVTIALVGKYIELPDAYLSVTEALRAGGFAHQAKVNIRWVASDLCESDEGATSQLADVDGICIPGGFGVRGIEGKLGALKFARENKIPTLGLCLGLQCMVIEFARNVLGLQDASSTEFDPQAKHPVIATMAEQIDILAAGEMGGTMRLGSFEAKLTKGSIAAEVYGSSEVTERHRHRYEVNNSYREQLTAGGMVISGTSPDGSLVEFVELSKADHPYFVATQAHPEFKSRPNRAHPLFIGLLQAAVEKKAA",
    "response#2": "MAKYIFVTGGVTSSLGKGIIAASLAKLLQARGLRVTLQKLDPYINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNVNTSQANNVTTGRIYQSVIEKERRGEFLGKTVQVIPHITDEIKERIQILGKSGDYDVVITEIGGTVGDIESLPYIESVRQLSWELGEHNSMVIHLTLIPYLSAAGELKTKPTQHSVKTLMESGVQADVLVCRTEHELSDEIRTKLARFCNVKPEAVIQSIDVDTIYAVPNKMLQEGLDQVVINQLELEAGPADLADWRNLVDSYKSIAEAFIHGGAVNDVEVDVEWRSAEDVEAGGTDFLERFHGVLIPGGFGERGIDGMIDTIRFVREREMPYFGICLGLQCAIIEFARNVCDLPTAHSTEFDPGTSDPVISLLDSQHQVTDMGGTMRLGAYPCLLQPGSRVHGVYGADEISERHRHRYEVNPAYRETLGAKGLVFSGMSPDGGLVEMLELPEHPYFLATQFHPELKSRPTKAHPLFAAFVESCLARQ",
    "response#3": "IFVTGGVVSSLGKGIASASLAAILEARGLRVTMMKLDPYINVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFVRTRLTGKNSITTGKIYEAVIRKERRGDYLGATVQVIPHITDEIKHSIFEATRGFDVALVEIGGTVGDIESQPFLEAIRQIRIEHGADKVMFMHLTLVPYIKAAGEIKTKPTQHSVKELRALGIQPDVLLCRCETPLPDSERRKIALFTNVPEKAVISAVDVDTIYAIPRWLHKQGLDELAVAHLKVEIALVGKYIALRDAYISVCEALRHAGLYHNVDVRIRWVAAEEVERRGPDALLRGVQGIVVPGGFGHRGVEGKIAAAGYARKNRIPFLGLCLGMQCATIEFAREALSAPDANSTEFDPHTSTPVIDLMADQRDVYEKGGTMRLGAYPCRLVQGTRVAEAYGIDLVHERHRHRFEFNNRYREALQQAGLLVAGTSPDGRLVEIVELRDHPWFVGTQFHPEFRSRPTRPHPLFVGFIGAALKKDAYKSLSEALRHGGLKQNTQVNLRWVESEEIEARGAEMLRDVDGILVPGGFGKRGFEGKIAAARYARENSIPYFGICYGMHAAVVDFARSIAGLPGANSSENDRLAKDPVIALITEWTKSSGEIEHRSEDSDLGGTMRLGAQEAKLKPGTLARELYGQDVIRERHRHRYEFNNNYRQKFADLGLVISGVSMDEELVEMIEFSPQKHPWFVAGQFHPEFTSTPRDGHPLFFGFIKG"
  },
  {
    "reference": "MQPTSSSPRVVLGVGGGIAAYKACELLRRLKSDDGIAADVTVVPTEHALRFVGAATFEALSGRPVYTDVFADVPSVAHVSTGQHADLVVIAPATADLLARAATGRADDLLTATLLVTRVPVLMAPAMHTEMWTHPATALNVETLRSRGVVVLDPAVGRLTGADSGAGRLPDPEHLAQVTELLLHRPDALPRDLVGRRIVVTAGGTREPLDPVRFLGNRSSGRQGFALALVAAARGADVTLLAANTALSVPPGVSMSEVGTAEELRRATLDAAARADAVIMAAAVADFRPADRHGSKIKKSAEHAARTLELTENADILAELVRRRNRAAHPVIVGFAAETGDASHTVAEYGRAKLARKGCDVLVVNDVSGGAVFGRADNAATILSDDGTEQAVPRGPKALLAAAVCDVVAHRLTSA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis pathway to avoid interference with native metabolic processes.2. The desired protein should be able to perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO23. The CTP binding site should be located in a region of the protein that is accessible to the ligand.4. The protein must be able to metal ion binding, phosphopantothenate--cysteine ligase activity. The designed protein sequence is ",
    "response#1": "MRIVLGVGGGIAAYKVASLLRLFTEAGHNVTVIPTEAANRFVGAATWEALSGNPVSNSVFDDVPSVNHVRLGHEADLIVVAPATADLLARAAGGHADDLLTNTLLMASGPVLMAPAMHTEMWLHAATRANVETLRGRGVTVLEPASGRLTGSDSGPGRLPEPEAIFAAAALAREALA",
    "response#2": "MAGLSVLVTSGGTREAIDPVRYIGNRSSGKMGNALASNASNRGADVTLVTTQEGPQDAALEIVRVETADDMAKAVWEKAPLVDVAVLAAAVADFAPRNPSTTKLRRADGAPKIELVATPDILAGVNESNPRPLLVGFAAEAGSLDAAAAKAAAKGVDLLVANDIQKTGSGFGSVTNEVRVLGADGSVTELPLMTKSQLADVILDRLT",
    "response#3": "KKILLGVSGGIAAFKAASLCSKLVQSGAEVKVIMTESATKFITELTLQALSRGPVYTDTFDEKDPAVISHIDLADWADLVLVAPATANIIAKMSVGIADDMLSTTLLATEAPIMLAPAMNVHMYQHPTVVRNLADLAARGVMMAEPGEGLLACGYVGKGRMEEPESLVRLAEAAASALGLKGRRVIVTAGATVERIDPVRYITNDSSGKMGFAIAAAARDLGADVHLIMGSTQASPPQGIPHTRVESAQDMYDALQEVWETADIVVKAAAVADYRPKEQFDRKIKKSGDQMSLELVKTVDILESLGRMKTTQFLIGFAAETNDLDKYAMDKLRRKNCDLLVANDVTQEGAGFGTDTNVVSLYWPGGEAVLPRLPKEALGRRLIELVAERWGE"
  },
  {
    "reference": "MKLIFATNNENKVKEFKSLVGDHLEIITLQDAGIDIDIPEPHDTLEANAREKSEVIHRMTNTNCFSEDTGLEIDALDGAPGVLSARYAGEQKLAADNIAKVLQEMQGVTNRAAQFRTVISLILDNKEYQFEGICSGTITTEVKGEKGFGYDPIFVPDGATKAFAEMDMAEKNLYSHRAKAFHQFAAFLNTIQA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein requires Mg(2+) to activate a critical catalytic site.2. The designed protein should have nucleotide binding, XTP diphosphatase activity, dITP diphosphatase activity to facilitate purine nucleoside triphosphate catabolic process.3. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "GWLNSFL",
    "response#2": "MPTPLVG",
    "response#3": "LFAA"
  },
  {
    "reference": "MNSQLSLSTIQSLVDGSVANPGSLLGRHPVNYRGREATSVRVLEPNAESVWLIDSASGLRRPMRRLHPGGFFEAICDEPITKPSTSRLQMIDKTGKEIKTTSPYTVPSIFSDLDRYLIGEGRHNQLYERLGAQLREVDGVKGVNFAVWAPNARSVQVVGDFNGWDGRGHVAQPVESTGIWELFLPGATVGQKYKFRIQTQHGHWMDKCDPMAFAAELPPLTANIITDINTYSWNDSDWLQQRAEMDPMHTPMNVYEVHLGSWQKGPGRTHGWLDYRDLAKRLVDYCHRMNFTHVELMPISEHPFTGSWGYQSVGYYAPTSRHGSPEDFMFFVDHMHQNGIGVLIDWVPAHFPKDDHGLRQFDGSALYEHADPRQGEHPDWGTMIFNFGRNEVKNFLIANALFWLDKYHIDGLRVDAVASMLYLDYSREDGEWIPNRYGGRENLESIDFLRDFNIAVHENHPGVITAAEESTAWPGVSRPTYDGGLGFTYKWNMGWMNDTLSYMRNEPIHRKFHQNELTFSLIYAFTENFTLPLSHDEVVHGKGSLISQMPGDMWQKFANLRLLYSYMWTHPGKKLLFMGGEIAQWTEWNADDGPQWELLDFDTHRGVQQLVADLNKVVIENPALHWHDFTGDGFEWIDAHNAEDSVLVYLRKGAEGDPPILVCNNFTPVPRDNYRVGVPAQGFWKEIFNSDGEAYGGSNLGNYPGCQTTGIEHHARPDSIEVTLPPLGTTILRLES",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that contributes to glycogen biosynthetic process by functioning in cytoplasm with 1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis).2. The protein should be optimized for activity in glycogen biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "EVHPGSWQKPHEEGFHTWDELADRLIPYVAEMGFTHIEFLPVSEHPYDPSWGYQTTGLYAPSARFGPPEGFARFVDGAHRAGISVLIDWVPAHFPTDEHGLVRFDGTALYEHEDPRLGFHPDWNTLIYNFGRREVVSFLVNNALFWAERYHVDGLRVDAVASMLYRDYSRKAGEWIPNAEGGRENWEAVEFLKAMNRAVYGSHAGFLTIAEESTAWPGVSKPAFDGAPRENLGFGFKWNMGFMHDTLKYMAREPIHRRYHHDEITFGLMYAFSENFVLPLSHDEVVHGKGSLLNKMSGDDWQKFANLRAYYGLMWGYPGKKLLFMGQEFAQRREWSEARALDWDLLQAPAHEGIRRWVRDLNRVYASRPALHARWLEVHPTSWMPAKCGDWNTLADRLIPYVQSLGFTHVEFLPVMEHPFGGSWGYQPLGQFAPMARLGTPESFASLVNRLHEVGIGVILDWVPAHFPTDAHGLARFDGTALYEHEDPREGYHYDWNTYIYNLGRNEVRAFLISSAVHWLTYFHADALRVDAVASMLYRDYSRKHGEWIPNMYGGRENLESVSFLQHLSTVVKSRAPGAVLIAEESTAWPGVTRPAHEGGLGFDYKWNMGWMHDTLHYMQEDPVNRSWHHDQMTFGLVYAFSEKFVLPLSHDEVVHGKRSLLGRMPGDEWQRFANLRAYYGFMWTHPGKKLLFMGGEIAQEQEWSEGHGTDWYLLDRPNHQGVQRLVADWLVGDDAANSVVAFLRKGKRGDAPVLVVINYTPVVQQGYRLGVPQGGLWREVFNSDAGIYGGANLGNGGAVTAEPQSMHGHAQSLPLLLPPLGVIVLA",
    "response#2": "ECHVGSWARRDDGWFLNYRDLAHRLGEYVTYMGYTHVELLGVMEHPFDGSWGYQVTGYYAPTSRLGSPEDFKYLVNHLHSLGIGVLLDWVPGHFPTDEFALAHFDGSPLYEYADPRKGYHYDWNTYIFDYGRNEVVMFLIGSALKWVQDYHVDGLRVDAVASMLYLDFSRTEWVPNIYGGRENLEAIAFLKRLNEVAHHMAPGCLMIAEESTSFPGVTTPTPEGLGFDYKWAMGWMNDSLAYFEQDPIYRKYDHHKLTFFNVYRTSENYVLAISHDEVVHLKKPMVLKHPGDWYAQRADYRAFLAMMWTTPGKKLLFMGQDFAQGSEWNHDAPLPWFHADQPDHRGVMNLVEVHPTSWMPAKCGDWNTLADRLIPYVQSLGFTHVEFLPVMEHPFGGSWGYQPLGQFAPMARLGTPESFASLVNRLHEVGIGVILDWVPAHFPTDAHGLARFDGTALYEHEDPREGYHYDWNTYIYNLGRNEVRAFLISSAVHWLTYFHADALRVDAVASMLYRDYSRKHGEWIPNMYGGRENLESVSFLQHLSTVVKSRAPGAVLIAEESTAWPGVTRPAHEGGLGFDYKWNMGWMHDTLHYMQEDPVNRSWHHDQMTFGLVYAFSEKFVLPLSHDEVVHGKRSLLGRMPGDEWQRFANLRAYYGFMWTHPGKKLLFMGGEIAQEQEWSEGHGTDWYLLDRPNHQGVQRLVADWNRRRPGLHLEVHLPSWRPGLTYRQLAEQLPAYVKDLGFTHVELMPVAEHPFGGSWGYQVTGFYAPTARLGTPDDFKYLVDALHQAGIGVLMDWVPAHFPRDDWALAEFDGRPLYEHEDPLRAAHPDWGTLEFDFGRREVRNFLVANAVYWCEEFHIDGLRVDAVASMLYLDYSREPGQWTPNVFGGRENLDAVAFLQEMNATVYRRVPGVVTIAEESTAWDGVTRPTESGGLGFGLKWNMGWMHDSLDYVQHEPVHRKYHHHEMTFSMVYAYSENYVLPISHDEVVHGKRSLVSKMPGDWWQQRANHRAYLGFMWAHPGKQLLFMGQEFAQGAEWSEAHGPDWWLLDPAYGAEPDHRGVRDLV",
    "response#3": "EVHLPSWRPGLTYRQLAEQLPAYVKDLGFTHVELMPVAEHPFGGSWGYQVTGFYAPTARLGTPDDFKYLVDALHQAGIGVLMDWVPAHFPRDDWALAEFDGRPLYEHEDPLRAAHPDWGTLEFDFGRREVRNFLVANAVYWCEEFHIDGLRVDAVASMLYLDYSREPGQWTPNVFGGRENLDAVAFLQEMNATVYRRVPGVVTIAEESTAWDGVTRPTESGGLGFGLKWNMGWMHDSLDYVQHEPVHRKYHHHEMTFSMVYAYSENYVLPISHDEVVHGKRSLVSKMPGDWWQQRANHRAYLGFMWAHPGKQLLFMGQEFAQGAEWSEAHGPDWWLLDPAYGAEPDHRGVRDLVRKAADDLESR"
  },
  {
    "reference": "MRQIDDAAHESEEVLIERAGYAIAAEALRMMGGGYGRRVTIIAGKGNNGNDGRAAARYLRRRGIRCNIQAPDSPTLPKSNLVIDAAFGTGFRDSWRPPSQPQCRVLAVDIPSGIDGLTGHDTGSLQADTTVTFGALKPGLLLQPGASRSGEIKIVDLGLDIGTSKNNLITDDDAAKVIATRSATAHKWTNAVRVVAGSPQMTGAAQLVTHGALRAGSGMVVASSPNTDLSLVPMPQEAVTRQHSEHSWHEEVLEDLHKFAALVIGPGLETDQETMTATAELISRAPLPVVIDAGALTAVATHPRCLSSRSHTTVLTPHDGEFETLTGMRPAVDRMSSLRRAIQDCTVLLKGPTTLVSNPLGSLFFVSSGDQRLATAGSGDVLAGIIGALIEHPEEHLAAAVGAHWHGHASLLAEPNMTAGDLPDLLAPARSVILQK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should contain one or more YjeF N-terminal domains that are essential for its function.2. The designed protein should have a high specificity for (6S)-NADPHX, AMP binding, with minimal off-target effects.3. The protein must exhibit the following characteristics: Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP; Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration..4. The protein have enhanced (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.5. A protein with ATP binding, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADHX epimerase activity, metal ion binding functions is required to enable nicotinamide nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "MVIALCGKGNNAGDAYLVAMLAKLKGLEAQCIHFVAPNTLSGDAKSAYEQCHETEVHIAAYNCDTFRILLDKQTSDCIIVDGLFGTGLNRDLDANYADAIELSNTVRREKSNIHTISLDIPSGLSANTGRIFNACIEADLTTTFIANKIGLMTGVGRHYSGQIAFHDLNLIEGIFGPGDLKYCLQKRPGESKKGDYGRIIIIGGSAEYSGAPALTALAALRTGADIVTVLVPERIEVPVRSFSPNLIVKSYPSDHLNRSALKENINLIEKHDVIAVGPGLGLNEDTILAVKEMLRLFDKPMVIDADGLKAIKGSLDLIYGKKAVLTPHAGEFKILFNAEPPRNLEERMKKVKELAGKYSTTILLKGHIDVISNGIKVKANPTGNPAMTVGGTGDVLTGIAATFLAHTEDPLYSAAGAAFLNGLIGDYVSSFKGAHITATDIIDYIPVILSRVSGPGDLKYCLQKRPGESKKGDYGRIIIIGGSAEYSGAPALTALAALRTGADIVTVLVPERIEVPVRSFSPNLIVKSYPSDHLNRSALKENINLIEKHDVIAVGPGLGLNEDTILAVKEMLRLFDKPMVIDADGLKAIKGSLDLIYGKKAVLTPHAGEFKILFNAEPPRNLEERMKKVKELAGKYSTTILLKGHIDVISNGIKVKANPTGNPAMTVGGTGDVLTGIAATFLAHTEDPLYSAAGAAFLNGLIGDYVSSFKGAHITATDIIDYIPVILSRGPGDLKYCLQKRPGESKKGDYGRIIIIGGSAEYSGAPALTALAALRTGADIVTVLVPERIEVPVRSFSPNLIVKSYPSDHLNRSALKENINLIEKHDVIAVGPGLGLNEDTILAVKEMLRLFDKPMVIDADGLKAIKGSLDLIYGKKAVLTPHAGEFKILFNAEPPRNLEERMKKVKELAGKYSTTILLKGHIDVISNGIKVKANPTGNPAMTVGGTGDVLTGIAATFLAHTEDPLYSAAGAAFLNGLIGDYVSSFKGAHITATDIIDYIPVILSR",
    "response#2": "MVIALCGKGNNAGDAYLVAMLAKLKGLEAQCIHFVAPNTLSGDAKSAYEQCHETEVHIAAYNCDTFRILLDKQTSDCIIVDGLFGTGLNRDLDANYADAIELSNTVRREKSNIHTISLDIPSGLSANTGRIFNACIEADLTTTFIANKIGLMTGVGRHYSGQIAFHDLNLIEGIFESKKGDYGRIIIIGGSAEYSGAPALTALAALRTGADIVTVLVPERIEVPVRSFSPNLIVKSYPSDHLNRSALKENINLIEKHDVIAVGPGLGLNEDTILAVKEMLRLFDKPMVIDADGLKAIKGSLDLIYGKKAVLTPHAGEFKILFNAEPPRNLEERMKKVKELAGKYSTTILLKGHIDVISNGIKVKANPTGNPAMTVGGTGDVLTGIAATFLAHTEDPLYSAAGAAFLNGLIGDYVSSFKGAHITATDIIDYITDDDVADLLPRPEAESDKYRRGVLGIVAGSDRFTGAAVLAVGGAIRAGAGLVRFASAETAVRQVRNRWPEAITTVLDGPSVAEVGRVQAWVLGPGMGTGEEAVALARAVLATDVPVLVDADGLTILANDRSLLRRSAPTVLTPHAGELSRLLDVPRERIEAARMEHVRRAVAELGAIVLLKGSTTLIAEQDRPVRVNTTGTPWLATGGTGDVLSGIAGTLLSGGLSGYDAVSCAAHLHGLAAQAASTGISGGHGPIAAMDVATMLPQVIESKKGDYGRIIIIGGSAEYSGAPALTALAALRTGADIVTVLVPERIEVPVRSFSPNLIVKSYPSDHLNRSALKENINLIEKHDVIAVGPGLGLNEDTILAVKEMLRLFDKPMVIDADGLKAIKGSLDLIYGKKAVLTPHAGEFKILFNAEPPRNLEERMKKVKELAGKYSTTILLKGHIDVISNGIKVKANPTGNPAMTVGGTGDVLTGIAATFLAHTEDPLYSAAGAAFLNGLIGDYVSSFKGAHITATDIIDYI",
    "response#3": "MSDDAQTNRDRLRHLQQAVVYELDKGDALPQVDHQCVVIDGLFGSGLNRPLAGRALELVQTVNSWKAEVVAIDIPSGLMGESNTDNHSDGIIKATQTFTFQFPKLSFLFPESAPYVGNWEVLPIGLHPDKIELYKNKAGRVAVIAGSIGMLGAAQLCTEAALRAGVGLVVLYCLPETYPLLAARVSPEIMVKQVQTYKNIDEDDAQALLIGPGLGQLDNANQLALHALVSCFDGTVVLDADGINLAAERSWRFEKNHILTPHPGEMRRLLPSAGMNTPRKELVNSFLYNHDATLVLKGARTIIGQRRQGMLYNSTGGPSMAKGGQGDVLAGICAGLAAQGMMPRDAAALAVYLCGRASELAVSRLKALDDVADPRVGCLLVGPGLGDIPQVLTLALTAPRPIVIDADAIGLVGEPERLHGHDVILTPHEGEFVKLFGELEGSKPERALAAASRSQSVVVYKGPDTLVASPDGRLGFAPPAPAWLASAGTGDVLAGIIATMRARGLPPFEAACAGVWLHGSAAERAGPRMIADDLIAALG"
  },
  {
    "reference": "MAHKKGASSSRNGRDSTSKRLGVKRFGGQFVHAGEILVRQRGTRFHPGSGVGRGGDDTLFALVSGAVTFGTARGRRVVSVVPAQPAATPAQPAATGS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in translation, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "MPRQYK",
    "response#2": "DTPPKK",
    "response#3": "LSL"
  },
  {
    "reference": "MAELTISSDEIRSAIEKYVSSYSPEVSREEVGVVTDTGDGIAHVEGLPSVMTEELLEFPGGIYGVAMNLEAQEVGAVILGESDKIEEGQEVRRTGKVLSVPVGDDFLGRVVNPLGEPIDGLGEITAEENRALELQAATVVQRQGVSEPMQTGIKAIDSMTPIGRGQRQLLIGDRKTGKTTVAVDTIINQKGNWESGDPQKQVRCIYVAIGQKGSTIAGVKRSLEEAGALEYTTIVAAPASDSPGLKWLAPYAGSAIGQHWMYQGKHVLIVFDDLTKQAEAYRAISLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDEMGAGSMTGLPIIETKANDVSAYIPTNVISITDGQCFLQSDLFNSGQRPAIDVGISVSRVGGSAQINAIRKVTGSLKIDLAQFRELEAFSAFASDLDATSKAQLDRGARLMEVLKQGQGEPLPVEEEVVSLFLGTKGHLDSVPVEDVRRFESEFLAHMRRTHDTVLKDIVETKKLSDDGAKTIADTAEEFKKQFTTSSGESAAPSEPEAEALAADEVGQETVKVNRPAPKK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: The alpha chain is a regulatory subunit..2. The protein should be able to bind ATP ligand in a variety of conditions3. The protein can be expressed in proton-transporting ATP synthase complex, catalytic core F(1), plasma membrane and perform ATP binding without triggering undesirable cellular responses.4. The desired protein should be able to perform ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate The designed protein sequence is ",
    "response#1": "KGGKVGLFGGAGVGKTVIIMELINNVAMKHSGYSVFAGVGERTREGNDLYYEMKESNVLDKVALCYGQMSEPPGARNRIALTGLTMAEYFRDEMGLDVLMFIDNIFRFAQSGSEMSALLGRIPSAVGYQPTLSREMGALQERITSTTKGSITSVQAVYVPADDLTDPAPASVFAHLDASRAMKQVAGQLRLDLAQYRELAAFAQFGSDLDKITQMRLTRGERMMEILKQKQYEPMVVEEQVVVLYAAVKGFLDDIPVDKIKSFEEDYLRTMRTTKADLLAKIRTEKALNDELNAEIEKAITEVKEGAGIIGERHYNLAQEVRRTLAKYDDLKDIIAMLGLEQLNVDDRKLVGRARRLERFFTQPFFSTEQFSTMEGKLVSLDDALEIKGGKVGLFGGAGVGKTVNMMELIRNIATEHSGYSVFTGVGERTREGNDFYHEMSDSRVLDKVSLVYGQMNEPPGNRLRVAFTGLTIAEKFRNEGHDVLLFIDNIYRYTLAGTEVSALLGRIPSAVGYQPTLSEEMGVLQERITSTNKGSITSIQAVYVPADDLTDPSPATTFSHLDSTITLSRQIVSLGIYPAIDPLNSTSRQLDPRIVGQLHYDVALGVRSILQRYQELKDIIAILGMDELSEDDKILVSRARKIQKFLSQPKGGKVGLFGGAGVGKTVNMMELIRNIATEHSGYSVFTGVGERTREGNDFYHEMSDSRVLDKVSLVYGQMNEPPGNRLRVAFTGLTIAEKFRNEGHDVLLFIDNIYRYTLAGTEVSALLGRIPSAVGYQPTLSEEMGVLQERITSTNKGSITSIQAVYVPADDLTDPSPATTFSHLDSTITLSRQIVSLGIYPAIDPLNSTSRQLDPRIVGQLHYDVALGVRSILQRYQELKDIIAILGMDELSEDDKILVSRARKIQKFLSQPL",
    "response#2": "MKKVAGTLKIDQAQFRELESFSKFGGEMDAVTAFTIDKGQKNTQLLIQPQYSPMPVEEQISILYCGTQGLLKEVPLDKVHEFEARFLQELRTSHQRDVLDVLKTGVINDEIRGILEKTAKEE",
    "response#3": "RGGKAGLFGGAGVGKTVLLTEMIHNMVSHHNGISIFCGIGERSREGEELYREMKEAGVLPNMVMIFGQMNEPPGARFRVGHAALTMAEYFRDDESRDVLLLIDNIFRFIQAGSEISGLMGQMPSRLGYQPTMGSELSSLEERIANTEDGAITSIQAVYVPADDFTDPAAVHTFSHLSASIVLSRQI"
  },
  {
    "reference": "MSPSKMNATVGSTSEVEQKIRQELALSDEVTTIRRNAPAAVLYEDGLKENKTVISSSGALIAYSGVKTGRSPKDKRIVEEPTSKDEIWWGPVNKPCSERTWSINRERAADYLRTRDHIYIVDAFAGWDPKYRIKVRVVCARAYHALFMTNMLIRPTEEELAHFGEPDFTVWNAGQFPANLHTQDMSSKSTIEINFKAMEMIILGTEYAGEMKKGIFTVMFYLMPVHHNVLTLHSSANQGIQNGDVTLFFGLSGTGKTTLSADPHRLLIGDDEHCWSDHGVFNIEGGCYAKCINLSAEKEPEIFDAIKFGSVLENVIYDEKSHVVDYDDSSITENTRCAYPIDYIPSAKIPCLADSHPKNIILLTCDASGVLPPVSKLTPEQVMYHFISGYTSKMAGTEQGVTEPEPTFSSCFGQPFLALHPIRYATMLATKMSQHKANAYLINTGWTGSSYVSGGKRCPLKYTRAILDSIHDGSLANETYETLPIFNLQVPTKVNGVPAELLNPAKNWSQGESKYRGAVTNLANLFVQNFKIYQDRATPDVLAAGPQFE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a fast turnover rate for ATP + oxaloacetate = ADP + CO2 + phosphoenolpyruvate.2. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.3. The protein design should consider the endogenous proteins and enzymes present in the Carbohydrate biosynthesis, gluconeogenesis pathway to avoid interference with native metabolic processes.4. A protein that can perform phosphoenolpyruvate carboxykinase (ATP) activity in cytosol to support gluconeogenesis. The designed protein sequence is ",
    "response#1": "NWVEEQAKLCKPDNIYICDGSEEEFKKFTDDMVKSGTLIKLNSKRPNSFLARSDPSDVARVESRTYICAKTKEDAGPTNNWMDPNQMKQTLKPLFNGSMKGRTMYVMPFSMGPLGSDISHIGVQVTDSPFVVCNMKIMTRMGDKVLNQIKENDDFVPCLHSVGAPLAKGQKDSHWPCNPNKYIVHYPEERSIQSFGSGYGGNALLGKKCFSLRIASSMAL",
    "response#2": "NWVEEQAKLCKPDNIYICDGSEEEFKKFTDDMVKSGTLIKLNSKRPNSFLARSDPSDVARVESRTYICAKTKEDAGPTNNWMDPNQMKQTLKPLFNGSMKGRTMYVMPFSMGPLGSDISHIGVQVTDSPFVVCNMKIMTRMGDKVLNQIKENDDFVPCLHSVGAPLAKGQKDSHWPCNPNKYIVHYPEERSIQSFGSGYGGNALLGKKCFSLRIASSMAF",
    "response#3": "NWVEEQAKLCKPDNIYICDGSEEEFKKFTDDMVKSGTLIKLNSKRPNSFLARSDPSDVARVESRTYICAKTKEDAGPTNNWMDPNQMKQTLKPLFNGSMKGRTMYVMPFSMGPLGSDISHIGVQVTDSPFVVCNMKIMTRMGDKVLNQIKENDDFVPCLHSVGAPLAKGQKDSHWPCNPNKYIVHYPEERSIQSFGSGYGGNALLGKKCFSLRIASSMAA"
  },
  {
    "reference": "MKSDPVSFDSSHDLMARPRHDWTRVEAETLYTTPFPDLLFQAQTIHRRHFDPNHVETASLLSIKTGGCPEDCGYCAQSAHYDAGVKATKLMDKDAVLETARRAKDAGAGRFCMAAAWRHPKDRDLDRVCDMISAVKALGMETCATLGMLTPEQARRLQEAGLDFYNHNVDTSPEFYDKIITTRTMQDRIETLACAREAGLKLCCGGIIGMGEQVGDRLGMLILLANLDEHPESVPINLWNEVRGVPVNDTADRPDPIALVRMIAVARIMMPKSVVRLSAGRQYMTDELQALCFVAGANSIFIGDVLLTTKNPATLRDAALLDRLGMSSPLDGVKMPAASPS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must bind to [2Fe-2S] cluster in order to perform its enzymatic function.2. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis pathway to avoid interference with native metabolic processes.3. The designed protein must be optimized for biotin synthase activity, iron ion binding. The designed protein sequence is ",
    "response#1": "FHGNRVDLCSIVNARSGRCSEDCAFCAQSGHYRTEAPVYPLLSKEEILERAREMELRGARRFALVTSGRGISESDFEKVLDIYQMLKEKTGLGLCASLGIIGYDKAVRLKEAGVGMYHHNLETCRSYFPHICTTHSFDERVETVKAAKEAGLEVCSGGIIGLGESWRHRVEMAFHLKELGVASVPINILTPVKGTPLWGRPLLEPVEVLRTAAMFRLVLPGALIRLCGGRE",
    "response#2": "FMGDKFDTCSIINAKSGNCPEDCKWCAQSRHYAASIEKYGLLSAAVCAEQAAYNRRQGIGRFSLVASGRTASMNEIRQMAESYRAIKQRTDIKCCASLGLLSEEKLQILFDNGVTTYHCNMETAPSFFPSLCSTHTQDEKLATIRAARRVGMRVCSGGIIGMGETMEQRIEFAFYLHSINVYSIPINILQPIPGTPLERTLPLSEEEYLTTVALFRLINPRAFLRFSGGR",
    "response#3": "FMGDKFDTCSIINAKSGNCPEDCKWCAQSRHYAASIEKYGLLSAAVCAEQAAYNRRQGIGRFSLVASGRTASMNEIRQMAESYRAIKQRTDIKCCASLGLLSEEKLQILFDNGVTTYHCNMETAPSFFPSLCSTHTQDEKLATIRAARRVGMRVCSGGIIGMGETMEQRIEFAFYLHSINVYSIPINILQPIPGTPLERTLPLSEEEYLTTVALFRLINPRAFLRFSGGQ"
  },
  {
    "reference": "MNVVVLAAGMGKRMHSRIPKVLHLLAGKPMLAHVVDTARALRPIRICVVYGHGGEAVPAAFGAADLQFARQDPQLGTGHAVQQALPYLQADARTLILYGDVPLVRSETLSRLTAASGDGLGVLTVSLDDPTGYGRILRDVDGRITGIVEEKDASPAQKAVREVNTGIMLVPAGKLGAWLGALKNDNAQAEYYLTDIVRFAIEDGVAVHGVEAAEEWETLGVNSKSQLARLERIYQRRQAESLLARGVTLVDPDRIDIRGTLEAGTDVRIDVNCVFEGAVRLGNDVTIGANCILNNVTVGDGSEILPFCHLAEADIGAANRIGPFSRVRPGALLGEDVHIGNFVEVKASELGKGSKVNHLAYVGDSTVGANVNIGAGTITCNYDGAYKHRTVIEDDVHIGSDVQLVAPVTVGRGATVGAGTTVWRNVPPGGLVLNKKEQLHRSDWTRPTKSRS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The presence of MobA-like NTP transferase domains is critical for the interaction of the designed protein with its intended targets.2. The protein should be designed to catalyze the following reaction acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.3. The designed protein should have a high specificity for acetyl-CoA, Mg(2+), UDP-N-acetyl-alpha-D-glucosamine binding, with minimal off-target effects.4. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).5. The designed protein should be involved in regulation of cell shape, lipid A biosynthetic process by localizing to cytoplasm and exhibiting UDP-N-acetylglucosamine diphosphorylase activity, glucosamine-1-phosphate N-acetyltransferase activity. The designed protein sequence is ",
    "response#1": "MSAILAAGRGTRMKSNLPKVLHSLGGRSLIGRVIDSVKPLSPTRQIVVVGYQAVEVQAAMESIPDLEFVEQTVQLGTGHAIQQLLPHLEGYTGDLLVLYGDVPLLRTETLKNLLQTHTQHQNAATILTLTLPDPTGYGRVIRNAQGEALAIVEQKDASAEQLQIREINTGILCAQAKDLKTWLSQLTNDNAQGEYYLTDIIAMAHQEGLHIQTVQPAHAFEVEGINNRVQLAALERAHQLRQAEQLMLSGVTLVDPEVTYIENGVEIGADTIIHPHVHLHGKTVIGSHCVIEPGAVIHDTRIADRVHIKASSVIERSEIGEDSQIGPMAHLRPGTVLRGHNKIGNFVETKKADIGLRSKASHLTYLGDSEIGADVNIGCGTITCNYDGVGKHKTIIEDQVFVGSDTQFVAPVHIGRNSLIGAGSTITRDVPPDALALSRAEQRHLTGWKRPVKAKK",
    "response#2": "VVILAAGKGTRMHSAFPKVLHRIGGRPMLLRVLDTAVSLSPXAIHVVVGVGAEQAIAIAGEAYPXAGINWVTQKEQLGTGHAVRVALPGLKQSDAGNSVLVLFGDVPLITRSTLKQVIDRCGPEQVSLLSAALDDPTGYGRILRRGSAVRAIVEEKDATEAQRAIREINSGIYAFDLNVLTAALGRIGNDNAQKEYYLPDVLQILVAAGAPVAALRCADPAEAMGVNDRVQLAEARRILNERIVEHWQREGVTMLDPGSVWIDAAVTIGKDTVLHPNVTLEGGTEIGEDCILRSHSRLTDCQVGDRVEILDHCVLRESRIEDDATVGPFVHLRPGVLVRRTAKVGNFVEMKKTDLGEGSKANHLSYLGDAKIGKGVNIGAGTITCNYDGVHKHQTTIGDNVFLGSDTQLVAPVTVGDGAVIAAGTTVTVDVPADSLAISREPQRNIDGWVLRKRPGTPSAAAAEKALQQRDSER",
    "response#3": "MTSVVILAAGKGTRMHSAFPKVLHRIGGRPMLLRVLDTAVSLSPXAIHVVVGVGAEQAIAIAGEAYPXAGINWVTQKEQLGTGHAVRVALPGLKQSDAGNSVLVLFGDVPLITRSTLKQVIDRCGPEQVSILTAVVEDPSGYGRILRDASGNVIAIVEEKDATPEERAVREINSGIYAFDAAVLREGLARLGTENAQGEKYLTDLLEIARGDGRKVAAYRARDAEEALGINDRIQLAEAARILNRRTVEAWMLAGVTFIDPSHAYVSSEAQIGRDCIIHPEVHIEGPSVIGEACEIHQGSRIVNSRIGDRVTVKDHCLIIDSEIANDCAVGPFAHLRMKAKLEERAVVGNFVEVKKSTIGRGTKAMHLSYLGDATIGEQTNIGAGTVTCNYDGKHKHPTIIEDNVRIGSDTMLVAPVTIGRGSVTAAGAVVTEDVPPNTLQRNIEGWTKRKRPGSAAAQAAQQAKDTD"
  },
  {
    "reference": "NMTVFRLNRDCTFPHPKHAESDGLLAVGGDLSAKRLINGYRDGIFPWYSEGDPILWWYTHPRFVIYPDRFTAGKRLMRYWKKTSYTFTIDQAFSQVIGLCGSSRTGRGEETWILPEMREAYGQLHELGYAHSVECWDVDRLVGGLYGVELGGVFFGESMFSTVSNSSKFCLIYLVEQARKRGVQLIDCQMTTRHLLQFGAVEISGEKFYQHLQLLIADIGPQPAWKI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with leucyltransferase activity is needed to enhance protein catabolic process in a specific context.2. The protein must exhibit the following characteristics: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl- tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine.. The designed protein sequence is ",
    "response#1": "EQFELLRRYLLTRHADGGMAEMTWPDYVAMVEDTAVRTHLIEYRRKSLDRGPGDLVACVLVDVLADGLSLVYSFYEPDQPRRSLGSFIILDHIVQAQQNALPYVYLGYWVPGSEKMAYKARFSPLEIL",
    "response#2": "EQYNLFRRYLDCRHQKGGMSDMSVLDYAMMVEDTHVHTKIIEYRLRVEGDGINDKARGPLIATALTDRMSDGLSMVYSFFDPALSERSLGTYMILDHIRRAKERGLPHVYLGYWVKGSRKMGYKTKFLPQEHC",
    "response#3": "EQYNLFRRYLDCRHQKGGMSDMSVLDYAMMVEDTHVHTKIIEYRLRVEGDGINDKARGPLIATALTDRMSDGLSMVYSFFDPALSERSLGTYMILDHIRRAKERGLPHVYLGYWVKGSRKMGYKTKFLPQEHC"
  },
  {
    "reference": "MTFLARLFGEKKKTAEIAKNRLSLLIAHERGDGVPKVDFLPALQRELIEVISKYVPVNSDDIKVHLDKQNNYEVLEVNIVLPEQNQGR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be able to modulate cell cycle, cell division in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "NMRRRATGYPAIVVPFLNGSLPQADLPPLRTIEDIDNLTREQCLTFIQGYGIPIDSSDTVYLKEKLRDAIGML",
    "response#2": "RGGRRLTGEIEIGGAKNSGLKLMVAGLLTSERLVLSNVPAIADIKTMRDLLVGLGITVEDAGPRTLSIGGEIGSVEAPYDIVSKMRASILVLGPLLARAREAKVSLPGGCAIGTRPVDMHLKGLETLGAEIRLENGYINATAPDGLKGDRIILPFASVGATENLLMAATLAKGRTHIVNAAREPEISDLVHCLNAMGAQITGEGSGTLVIDGVEKLHGAHYAVMPDRIECGTYACAAGITGGDLLLVGGRADDLGAVIRTLEETGVEVLEEERGLRVRRSGTLQGVDIMTEPYPGFPTDMQAQFMAMLSVAEGASMITETIFENRFMHVPELNRMGARVNVHGRSAIIRGVPKLSGAPVMATDLRASFSLILAGLAAEGETQLSRIYHLDRGYEGVDRTGL",
    "response#3": "NMRRRATGYPAIVVPFLNGSLPQADLPPLRTIEDIDNLTREQCLTFIQGYGIPIDSSDTVYLKEKLRDAIGMI"
  },
  {
    "reference": "MIITRNWLKKYLNLDNISNDQINVALNSLGFEVDNVYDLNSLNSELVLGYVEQSKQIPDTHLKLNKVNIGTKSLQIVCGASNVDVNQFVIVAPINATIANGLTLTSKKIQNYESQGMICALNEIGINQSVINKEDQLKIYNVFDKNLDLKKYLGKDVKQIIGLDDYLFEIDLTLNRSDCLASFQILKELANYFDLEIKNYDNKFNDFKENDLDLKITISKKIEEQIKTISYSNFVLNNHLNKLDSIDDIFLKLNQISSTNNLINDLSLLSTLSTAQTHILIDLDKLKSFNLKLELINHNDKELLCLTSDNQIVNIIGLQTESKFSIDNNSKNVLNIMVNIEPNLMRKQQKLLNTSNINLQRYIKPINPNLFDLANLNLTSLLNSYNLVNKAYKVKVLKQTYKNKTEFEIKISEINDLLGTNLTIDQIKSLFKKLDFKITNKNDLLTFNIDPNRIDISSKNDLCEEVARLYSYDNIQEVALSFTSFKKPKNLNLKLENKLTNYLIGLGFNNTKTYSLTTQIDAKHWNLFNISDFINLLSPLSNLRQTYRTSLSKSLIDTAIYNHSINNKELKLFEIADIYNLNQLKQRHLVFLTSHHIYKNGLTHQLVENNFYYNKEILESIFDLYNLDFSQIKYVNNLDVIKEIHLYINATIYYQKQLVGFIYQLNPKFESENKLNKTFVCEINLDVLNELKNKTIEAKTLSKFQSSSRDLTIEISNDLIYQDVLLKAISDVKYITSSKVVDLYLDDKLAKNNTKALTIQFIFNDLDH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a high specificity for Mg(2+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MVAHMNEEALRLTLETGIAHYWSRSRKTLWKKGETSGNLQSVVELRTDCDQDALWLKVHVAGDGPTCHTGRRSCFS",
    "response#2": "MVAHMNEEALRLTLETGIAHYWSRSRKTLWKKGETSGNLQSVVELRTDCDQDALWLKVHVAGDGPTCHTGRRSCFH",
    "response#3": "KDLDFTRKLPFARDRVVEGYFWIMGVYFEPQYSLGRKMLTKVIAMASIVDDTYDSYATYDELIPYTNAIERWDIKCMNQLPNYMKISYKALLNVYEEMEQLLANQGRQYRVEYAKKAMIRLVQAYLLEAKWTHQNYKPTFEEFRDNALPTSGYAMLAITAFVGMGEVITPETFKWAASDPKIIKASTIICRFMDDIAEHKFNHRREDDCSAIECYMKQYGVTAQEAYNEFNKHIESSWKI"
  },
  {
    "reference": "MPRVKRGFKARQRRNKVLKLAKGYRGARSKLFRSATEAVDRALNYAFRDRRVKKRDFRALWITRINAAARINGLSYSKLIHGLKLANVEIDRKVMADLAVSDPNGFAAIAAAAKAKF",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be optimized for stability and solubility when expressed in ribosome, ribonucleoprotein complex where it will be involved in ribosomal large subunit assembly.2. For general function, the protein need meet that It is not involved in the protein synthesizing functions of that subunit.; Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit The designed protein sequence is ",
    "response#1": "MPIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTY",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTE",
    "response#3": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG"
  },
  {
    "reference": "MPFDRVEDAIEDIRQGKLVIVADDEDRENEGDLVCAAAKITPDLVNFMATHGRGLICVAVTPERAAELDLKPMTDENSAPFGTAFLVSVDAHPRFGVTTGISAHDRATTIRLLVDPATKPEDLVRPGHIFPLRAQPGGVLRRVGQTEASVDLARLAGLPPVGVLCEILNPDGTMARRPDLEAFAQRHGLRFITVAQLIAYRLQHERLVHREAEATLPTAYGEWRIIAYRNDVDAHIHVALVKGNVAGEEPVLVRMHSECLTGDIFHSLRCDCGEQLESSMRMIDEAGRGVVVYLRQEGRGIGLAHKIRAYRLQEEGLDTVEANEALGFRPDLRDYGIGAQILLDLGLRRLRILTNNPKKIIGLQGYGLEVVEQVPIPVTPNPYNRRYLQTKRDKLGHLLPL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Proton acceptor; for GTP cyclohydrolase activity active site of the designed protein must be optimized for substrate binding and specificity.2. The protein should be able to bind Mg(2+), D-ribulose 5-phosphate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "KMAETNLPTEYGKFKLFVYNSDVDYLPHLALVLNKITNPSLVRVHSECLTGDVFMSKRCDCGRQLHKSMRIIQKNGQGVLLYMRQEGRGIGLTNKIKAYALQDHGFDTVEANEKLGFKPDLRDYGIGAQILSDLGIQKIRLLTNNPYKIIGLEGYGIEIVERVR",
    "response#2": "KMAETNLPTEYGKFKLFVYNSDVDYLPHLALVLNKITNPSLVRVHSECLTGDVFMSKRCDCGRQLHKSMRIIQKNGQGVLLYMRQEGRGIGLTNKIKAYALQDHGFDTVEANEKLGFKPDLRDYGIGAQILSDLGIQKIRLLTNNPYKIIGLEGYGIEIVERVR",
    "response#3": "REAEVRLPTAHGDFTAYGYRSAADGVEHVALVHGDLGDGEDVLVRVHSECLTGDVFHSLRCDCGPQLHAAMDRVTEAGRGVVVYLRGHEGRGIGLLSKLRAYELQERGRDTLDANLELGLPADARDYAAGARMLADLGVRSVRLMTNNPDKSAALARHGIVVSGG"
  },
  {
    "reference": "MLSRLQELRKEEETLLRLKAALHDQLNRLKVEELALQSMISSRRGDEMLSSHTVPEQSHDMLVHVDNEASINQTTLELSTKSHVTEEEEEEEEEESDS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Recruits TBP and BRF2 to the U6 snRNA TATA box.; Part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes2. The protein should have a specific function in snRNA transcription by RNA polymerase II, transcription initiation at RNA polymerase III promoter and should be localized to nucleoplasm, nucleus, nucleolus in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "LVTKWKNWEKALLRKSVVSDRLQRLLQPKLLKLEYLHQKQSKVSSELERQALEKQGREAEKEIQDINQLPEEALLGNRLDSHDWEKISNINFEGSRSAEEIRKFWQNSEHI",
    "response#2": "LVTKWKNWEKALLRKSVVSDRLQRLLQPKLLKLEYLHQKQSKVSSELERQALEKQGREAEKEIQDINQLPEEALLGNRLDSHDWEKISNINFEGSRSAEEIRKFWQNSEHV",
    "response#3": "LVTKWKNWEKALLRKSVVSDRLQRLLQPKLLKLEYLHQKQSKVSSELERQALEKQGREAEKEIQDINQLPEEALLGNRLDSHDWEKISNINFEGSRSAEEIRKFWQNSEHW"
  },
  {
    "reference": "MTELKAKEPRAPHVAGGAPSPTEVGSQLLGRPDPGPFQGSQTSEASSVVSAIPISLDGLLFPRPCQGQNPPDGKTQDPPSLSDVEGAFPGVEAPEGAGDSSSRPPEKDSGLLDSVLDTLLAPSGPGQSHASPATCEAISPWCLFGPDLPEDPRAAPATKGVLAPLMSRPEDKAGDSSGTAAAHKVLPRGLSPSRQLLLPSSGSPHWPAVKPSPQPAAVQVDEEDSSESEGTVGPLLKGQPRALGGTAAGGGAAPVASGAAAGGVALVPKEDSRFSAPRVSLAEQDAPVAPGRSPLATSVVDFIHVPILPLNHAFLATRTRQLLEGESYDGGAAAASPFVPQRGSPSASSTPVAGGDFPDCTYPPDAEPKDDAFPLYGDFQPPALKIKEEEEAAEAAARSPRTYLVAGANPAAFPDFQLAAPPPPSLPPRVPSSRPGEAAVAASPGSASVSSSSSSGSTLECILYKAEGAPPQQGPFAPLPCKPPGAGACLLPRDGLPSTSASGAAAGAAPALYPTLGLNGLPQLGYQAAVLKEGLPQVYTPYLNYLRPDSEASQSPQYSFESLPQKICLICGDEASGCHYGVLTCGSCKVFFKRAMEGQHNYLCAGRNDCIVDKIRRKNCPACRLRKCCQAGMVLGGRKFKKFNKVRVMRALDAVALPQPVGIPNESQRITFSPSQEIQLIPPLINLLMSIEPDVIYAGHDNTKPDTSSSLLTSLNQLGERQLLSVVKWSKSLPGFRNLHIDDQITLIQYSWMSLMVFGLGWRSYKHV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Transcriptional activator of several progesteron-dependent promoters in a variety of cell types; The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues; Involved in activation of SRC-dependent MAPK signaling on hormone stimulation.2. Incorporating a Nuclear localization signal motif is critical for the successful design of the protein.3. The presence of NR LBD domains is critical for the interaction of the designed protein with its intended targets.4. The ideal protein would be one that plays a critical role in regulation of epithelial cell proliferation, ovulation from ovarian follicle through zinc ion binding within nucleus, cytoplasm. The designed protein sequence is ",
    "response#1": "VCEAASAGLLKTLRFVKYLPCFQVLPLDQQLVLVRSCWAPLLMLELAQDRLNFETVETLEPSLLQMILTTRRQETEGDEPPSPQPPVQPHLVLPSEAEHLPSVAEVQAIKGFLAKCWSLDISTKEYAYLKGTVLFNPDLPGLQCVKYIQGLQWGTQQILSEHIRMTHRGYQARFAELNSALFLLRFINANVLAELI",
    "response#2": "STRLMTTLNRLGGRQVISAVKWAKALPGFRNLHLDDQMTLLQCSWLFLMSFSLGWRSYQQCNGNMLCFAPDLVINEERMKLPYMADQCEQMLKISSEFVRLQVSHDEYLCMKVLLLLSTVPKDGLKSQTVFDEIRMSYIKELGKAIVKREENSSQNWQRFYQLTKLLDSMHELK",
    "response#3": "QRSSILYSVARSANQTHAAPEMQPRAPWWDTSCDALRPVALKSPQVVCEAASAGLLKTLRFVKYLPCFQVLPLDQQLVLVRSCWAPLLMLELAQDRLHFETVETSEPSMLQRILTSRRQETAGNEPPPLSTPQSHLAPPPEARPMPSAAEVQAIKGFLAKCWSLDISTKEYAYLKGTVLFNPDLPGLQCVKYIQGLQWGTQQILTEHVRMTHRGHQARFAELNSALFLLRFINANVIAELFFRPIIGTVSMDDMMLEMLCAR"
  },
  {
    "reference": "MDTLVLPPLHTASGCVRLPGSKSISNRMLLLAALAEGTTEIHDVLVADDTQVMLSALQKLGVGMTAIDDHTWRIKGCGGDFPVAEADLFMGNAGTVIRPLTAALALSGGHYRLSGVPRMHERPIGDLVDGLRQIGADVRYTGEAGFPPLEIFPVTIQLAQPIRLRGDVSSQFLSALLMALPLSGQAAVIEITTELISQPYVEITLNLMARFGVKVQREGWQRFILLAGQRYRSPGVLHVEGDASSASYFLAAGLLGGGPVRVEGVGKNSIQGDVQFAYALESLGAHIVWGENFIEAKAESSAAEKIKAFDLDLNHIPDAAMTLAVCALFADGRCVLRNIGSWRVKETDRITAMATELRKLGAVVETGGDWLAISPAPALCLPCTPFGAPSGTFRRNVAIDTYDDHRMAMCFSLAAFAGVSIIINDPGCVRKTFPGYFAALAEISAPVIALDGPSASGKGTVASMVAAQLGYHYLDSGALYRLTALAALRAGVALDDAPRVAALAAALPARFENGRVFLNEDDVTDAIRTEEASAGASRVAVLADVRAALLARQRAWRRFPGLVADGRDMGSVVFPSATVKVFLTASAEARAERRYKQLIEKGSAANMPALLHDLQARDERDAQRSEAPLRQCEDALQVDTTLMDIAEAVNAVMAIVEEKLPDAV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.2. The designed protein should be involved in chorismate biosynthetic process, pyrimidine nucleotide metabolic process, amino acid biosynthetic process, aromatic amino acid family biosynthetic process, phosphorylation by localizing to cytoplasm and exhibiting CMP kinase activity, ATP binding.3. A protein that is capable of catalyzing reactions within the chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7 pathway with high efficiency and specificity.4. The presence of Enolpyruvate transferase domains is critical for the interaction of the designed protein with its intended targets.5. The designed protein is the optimization of the Proton donor to achieve high catalytic efficiency for ATP + dCMP = ADP + dCDP.6. The designed protein should have a high specificity for phosphoenolpyruvate, ATP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "TMPASKSFAQRAILAAALAEGTSHLGGYSPCGDSEAALALAGALGAKVRKEGSTLAITGIGARCRQLQPGTVDTGESGLLTRLTIPVLAAVGDGSFTVTGRGTLLRRPLSGAADIMAAFGVLLASEAEGAGKEVRIPATVKGHLIPGTAEVPGQGGSQLISGLLMALPLCDKDSLLRVSEPKSIPYMYITLDVLRHFGIQTRSEMEGDAEMLEADDWSACTGINFKVRGRQHYRAADFDIEGDWSAAANLLVAGAVFGSAELVGMDTKSLQADLTIIDILVEAGAVVSQLEEGTVCVRKAPLEAFQQDLNNAPDLFPIVAVLAAFCAGESRIAGVGRLATKESNRAEAILQMLLQMGVEARVEGDELVVWGESLAARLLGGRLLKGGDYTSRHDHRMVMALKVASLGAESPIIIDDEACVGKSFPDALR",
    "response#2": "TMPASKSFAQRAILAAALAEGTSHLGGYSPCGDSEAALALAGALGAKVRKEGSTLAITGIGARCRQLQPGTVDTGESGLLTRLTIPVLAAVGDGSFTVTGRGTLLRRPLSGAADIMAAFGVLLASEAEGAGKEVRIPATVKGHLIPGTAEVPGQGGSQLISGLLMALPLCDKDSLLRVSEPKSIPYMYITLDVLRHFGIQTRSEMEGDAEMLEADDWSACTGINFKVRGRQHYRAADFDIEGDWSAAANLLVAGAVFGSAELVGMDTKSLQADLTIIDILVEAGAVVSQLEEGTVCVRKAPLEAFQQDLNNAPDLFPIVAVLAAFCAGESRIAGVGRLATKESNRAEAILQMLLQMGVEARVEGDELVVWGESLAARLLGGRLLKGGDYTSRHDHRMVMALKVASLGAESPIIIDDEACVGKSFPDVCIDGPTASGKGTLAAEVARRLGYHYLDSGALYRVTGLAVRRMGLEPEPRHEAAIAQAAATLPLSFTAGQVWLAGEDVTEAIRSEAAGMDASRVSALASVRAALRTLQEGFRKLPGLVADGRDMGTVIFPAAALKVYLTASAAQRAERRHKQLISKGISTTLDSLRADLEARDARDSSRSVAPLKPAQDARLLDNSTLSIEQSVEQVLDESALAGRVVCIDGPTASGKGTLAAEVARRLGYHYLDSGALYRVTGLAVRRMGLEPEPRHEAAIAQAAATLPLSFTAGQVWLAGEDVTEAIRSEAAGMDASRVSALASVRAALRTLQEGFRKLPGLVADGRDMGTVIFPAAALKVYLTASAAQRAERRHKQLISKGISTTLDSLRADLEARDARDSSRSVAPLKPAQDARLLDN",
    "response#3": "MTICIDGPTASGKGTVAAAVAQKLGYRFLDSGAMYRITAHAAIRAGLAIDAAHEEQIAALARELPVHFHGGRIWLDQDDVSDAIRTEEAGMNASRVSALPQVRTALIDLQHSFQRLPGLVADGRDMGTVIFPQAPLKVFLTASAACRAERRYKQLISKGISANIDTLRADLEARDARDQSRAVAPLKPAEDALVLDNSDLSIDEAVDRVIELASQELSATGTVQLPGSKSISNRVLLLAALCEGQTTVLHDVLASDDTDAMLTALQTLGCEIERQAQQVRITGLDRQSLPAQAQLHLGNAGTAMRPLTAALALLVGPGQAFELTGVARMQERPIGDLVDALRQLGCAIDYLKNPGFPPLALRAADPQKLQASGSIQIRGDVSSQFLTAMLMALPLVAKERDYVIEVVGELISKPYIAITLNLLARFGIVVRNDNWQRFTIAAGSRYQSPGQLHVEADASSASYLVALGGIASDPALGQAITIEGVGEDSIQGDIRFIDAARQMGVHVETGPNWLRVQRGQWPLKAIDIDGNHIPDAAMTLAVMALYAEGTTRITNIASWRVKETDRIDAMVNELSKLGAVVDSGPDWISVAPPAADAWRSASIATYDDHRVAMCFSLAAFNPASVAVRIEDPRCVGKTFPDYFEALGLS"
  },
  {
    "reference": "MGHLKLKSIEVDSTVKKLGDPLKFNLRFVCFEEIKCGVEFVVLYNMDVHSDENDQVLAEIEVAPIPKGKIEFSIDADAPDVNKIPLDEMFGLTSILIVGRYKGQQFIRIGYIVDVGYPGIPSTKLMKSDVEEPSEEIGDKEEEDEEDVEEELGSEEGSEESSCIVEDKDEDNEEAEPRTFMEAVEDVGNEGKERLFESEGREEEEDEKVGSDSYSEVNRELNKSVGEEAEGSDGGEDVVDYCGFRIDKKQIEMKLMDPPVINLFEIEWEEESPSEEVPRNNNESPAKKQKVE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have histone binding to facilitate nucleosome assembly.2. The protein should exhibit Acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "METQIHLIEQEAYSSILRAFKAQSDAITWEKESLITELRKELRVSDEEHRELLSRVNADEMIRRIREWRKANSLQSSVPQLVHDAPSS",
    "response#2": "METQIHLIEQEAYSSILRAFKAQSDAITWEKESLITELRKELRVSDEEHRELLSRVNADEMIRRIREWRKANSLQSSVPQLVHDAPSNA",
    "response#3": "PPTDGVKEPHRYRPGKMGLREIRKYKKNARFFISKLPFHRLVRKITQNLKAHLRFQSTAMPAPEEVSEAYLVKLFEDAIHAKRVTLVPKDIQLARCR"
  },
  {
    "reference": "MTEYLGTFNGSKLKIAIIVARFNDLVTKRLLDGAFQTLAQNGVSKEDIDIYWVPGAFEIPRVAQKISQKGNVDGIITLGAVVRGETSHYESVCSGVTSGIAQIALEGKVPVMFGVLMTENMEQALNRAGGKAGNKGSECATGLLEMIDIEQTIDRE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a high specificity for 5-amino-6-(D-ribitylamino)uracil, (2S)-2-hydroxy-3-oxobutyl phosphate binding, with minimal off-target effects.2. We require a protein that can specifically bind to the metabolites produced in the riboflavin biosynthesis, Cofactor biosynthesis pathway.3. The protein must exhibit the following characteristics: This is the penultimate step in the biosynthesis of riboflavin..4. A protein with 6,7-dimethyl-8-ribityllumazine synthase activity is needed to enhance riboflavin biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "MPRGSITLATGNAGKVAEFAALIAPFGVRVVPQSAFDVPEAEEPHRTFVENALAKARHAARLSGLPAVADDSGIAVDALGGAPGVHSARYAGDASDDANNRKLLAALEGTPDERRGARFVCVIVLMRHAEDPTPVIAHGTWSGRVLTAPRGTGGFGYDPLFVPDGETRTCAELSPAEKNAISHRGQALRAMLAALPAT",
    "response#2": "MPKNMIAFDMDGTLIDSAPDIAVAADELLRPLGRAEISHEDIRSWVAHGATVLIERAFRLSGHENDPELFAKARERFIGLYEQRLAQTSVLFPGVKEGLDALVDAGIPLALVTNKPQAFVEPLLAALGIGRFFSLILGGDTFPAKKPHPEPYLHCMELLGVLPAEALMVGDSRNDILAGRNAGVWTCAVTWGYHPGKPLPEGVQPDWIIEHFTELLPLIKEAR",
    "response#3": "MNMQHVIIATSNPGKLAEFKKIAAELPLKIRTLGEIGCLEDIVETGTTFEENARLKSEAYLDATGKPSLADDTGLEVDALGGKPGVHSARYAGTDASDEENNRKLIKELEGKNNRRARFRSAIAYFDGKSEKYFEGICEGKISEERRGTEGFGYDPVFIPEGFEKTFAELGEETKNKLSHRGKAFEKLFGFLKQ"
  },
  {
    "reference": "MYSQTKIAIPIFQAKKEDVIKVAEDCIEKGADVLEFRIDALDNPNFDDIKEIIEEINFPIIATNRISSEGGSFKGSEEERIDILLKCAPLVDYVDIELQSDDRYIKQIHDTGVTTIVSYHDFHKTPEINEIMYIVEKEQKLGDIAKVAFMPNDLDDTLKILAILSHCENTIAISMSDLGSYTRVMASKFDSPITFAAGRDVTAPGQIDIETMKSLLNMDLNLME",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for 3-dehydroquinate binding.2. The protein must exhibit the following characteristics: Catalyzes the cis- dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate.; Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids.3. The designed protein should interact with enzymes and substrates within the Metabolic intermediate biosynthesis pathway to enhance or regulate its activity.4. The Schiff-base intermediate with substrate, Proton donor/acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.5. The designed protein must possess 3-dehydroquinate dehydratase activity to support amino acid biosynthetic process, chorismate biosynthetic process, aromatic amino acid family biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "MVTPVEGKTLVLLGAGGSSRAIAFEAIRRGARVMILNRTPEKAIKLANHLGCCGGSLGDLEKISYDILINTTPHSCPIDLLYGEVVLPGLRKEVTPVEGKTLVLLGAGGSSRAIAFEAIRRGARVMILNRTPEKAIKLANHLGCCGGSLGDLEKISYDILINTTPHSCPIDLLYGEVVFPGLKGNAAQMASL",
    "response#2": "MVTPVEGKTLVLLGAGGSSRAIAFEAIRRGARVMILNRTPEKAIKLANHLGCCGGSLGDLEKISYDILINTTPHSCPIDYVELREGVRVTPVEGKTLVLLGAGGSSRAIAFEAIRRGARVMILNRTPEKAIKLANHLGCCGGSLGDLEKISYDILINTTPHSCPIDYVELREGVRVTPVEGKTLVLLGAGGSSRAIAFEAIRRGARVMILNRTPEKAIKLANHLGCCGGSLGDLEKISYDILINTTPHSCPIDYVELREGVRVTPVEGKTLVLLGAGGSSRAIAFEAIRRGARVMILNRTPEKAIKLANHLGCCGGSLGDLEKISYDILINTTPHSCPIDYVELREGVR",
    "response#3": "MVTPVEGKTLVLLGAGGSSRAIAFEAIRRGARVMILNRTPEKAIKLANHLGCCGGSLGDLEKISYDILINTTPHSCPIDTEIVTPVEGKTLVLLGAGGSSRAIAFEAIRRGARVMILNRTPEKAIKLANHLGCCGGSLGDLEKISYDILINTTPHSCPIDTEIMPRNVRVNDIEMFRDQAAVAKLTKLAHQKKVLVVMSNHCSAAELKGSCK"
  },
  {
    "reference": "MKIHSSDHSWLLLAVLVILLSFSGALSSDDKEKKTKSVDKKKEVTYDGTSLIINGNRELLYSGSIHYPRSTPEMWPNIIKRAKQGGLNTIQTYVFWNVHEPEQGKFNFSGRADLVKFIKLIEKNGLYVTLRLGPFIQAEWTHGGLPYWLREVPGIFFRTDNEPFKEHTERYVKVVLDMMKEEKLFASQGGPIILGQIENEYSAVQRAYKEDGLNYIKWASKLVHSMDLGIPWVMCKQNDAPDPMINACNGRHCGDTFPGPNKDNKPSLWTENWTTQFRVFGDPPAQRSVEDIAYSVARFFSKNGTHVNYYMYHGGTNFGRTSAHYVTTRYYDDAPLDEFGLEREPKYGHLKHLHNALNLCKKALLWGQPRVEKPSNETEIRYYEQPGTKVCAAFLANNNTEAAEKIKFRGKEYLIPHRSISILPDCKTVVYNTGEIISHHTSRNFMKSKKANKNFDFKVFTESVPSKIKGDSFIPVELYGLTKDESDYGWYTTSFKIDDNDLSKKKGGKPNLRIASLGHALHVWLNGEYLGNGHGSHEEKSFVFQKPVTLKEGENHLTMLGVLTGFPDSGSYMEHRYTGPRSVSILGLGSGTLDLTEENKWGNKVGMEGERLGIHAEEGLKKVKWEKASGKEPGMTWYQTYFDAPESQSAAAIRMNGMGKGLIWVNGEGVGRYWMSFLSPLGQPTQIEYHIPRSFLKPKKNLLVIFEEEPNVKPELIDFVIVNRDTVCSYIGENYTPSVRHWTRKNDQVQAITDDVHLTANLKCSGTK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be designed to catalyze the following reaction Hydrolysis of terminal non-reducing beta-D-galactose residues   in beta-D-galactosides..2. The protein should be able to modulate carbohydrate metabolic process in a way that leads to a desirable outcome.3. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "LKNDNLFTNSSSYYFLTSLLVDGKLTYQSRPLTFGLEPGESGTFALPWPEVADEKGEVVYRVTAHLKEDLPWADEGFTVAEAS",
    "response#2": "DTSWIRPGPALWTWLAGGKPAGQSLSMQKGYVDYAAQRGWPYVVVDAGWYFDPDQWDVTDPDWQTNSWIPELVTYARERGVGIQVWIHHRDLDTAEEREQWLPTLERWGVKGVKIDFMDSESQDTLRWYDEILPATAAHHLLVNFHGSTIPKGIQRTWPHVMTMEGVNGEEKRVNTAQHLTTLPFTRNVIGSMDFTPGAFHRPQRPNAASDAGELGLSVLYESGIQNLAGTPESYDARPLARGFLEQLS",
    "response#3": "DTSWIRPGPALWTWLAGGKPAGQSLSMQKGYVDYAAQRGWPYVVVDAGWYFDPDQWDVTDPDWQTNSWIPELVTYARERGVGIQVWIHHRDLDTAEEREQWLPTLERWGVKGVKIDFMDSESQDTLRWYDEILPATAAHHLLVNFHGSTIPKGIQRTWPHVMTMEGVNGEEKRVNTAQHLTTLPFTRNVIGSMDFTPGAFHRPQRPNAASDAGELGLSVLYESGIQNLAGTPESYDARPLARGFLEQLK"
  },
  {
    "reference": "MPLFILTETSAGYGLFKAADKKLLSSDNLAERLNSVEKITKEIKYKEFAKFESAATALEEIAGVVEGKVTPKLNSLLAEIGNEKKVTLAVAESKLGAAINKIPNLDIQPIADSTTTDLFRAIRQYLPELIPGMLPENFKEMSLGLSHSLSRHKLKFSPEKVDVMIVHAVSLLDELDKELNTYAMRVKEWYGWHFPELAKILPDNLSYARIIVTMGMRSNATTADLSEILPHEIEAAVKAAADISMGTEVSEEDLQNIKYLAERVIDYSVYRKQLSDYLENRMRAIAPNMTELVGALVGARLIAHAGSVMNLAKNPGSTIQILGAEKALFRALKTKHATPKYGLIYHASLVGQASGANKGKMARQLASKVALGVRTDALAEFPEDADDETRASLGIRSRAKLENNLRQLEGKPIASKGVSVGPNGIPVASAPKWDIKEARKYNIDADGLAADAEAPKKPLIQEVEMQDAPADEDEEMKDVSKKSKKDKKEKKEKKDKKAKKELDDEDFERLAKAAGMSVDKFKRRFERGQISIKEDGTLEILSKKDFKGKDAEAEEEAEEEEKPAKKSKSSKRKAEAEEEETTKEEKPKKKKKKSSKE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should exhibit Basic and acidic residues compositional bias.2. The protein must exhibit the following characteristics: May bind microtubules .; Required for pre-18S rRNA processing.3. The ideal protein would be one that plays a critical role in endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA), snoRNA guided rRNA 2'-O-methylation through snRNA binding within nucleolus. The designed protein sequence is ",
    "response#1": "LEFLVPIILEIAAKLLASGSTDCQIAAHTILIVFTAALPLKKDIVVAAIETILANLKDKKAEQSAFWAVIKISQTLKGQQAIEHLPANIYKIFDKHYTWVRVLDLLTEEPSIKCDKFMTSYIRR",
    "response#2": "MPSLSQPFRLATLPKIASLSNFSLQADYVQVADGTFNESTNNITLGISGSSISQYIINPTPKLTFDYPIPSTNIITACNAEKGQANIDGNIEASTDDEANNEKTINTQKKRNVEIWAFGLMVNKGNYTLNVITKALEDTTDTSNDHLSESDIDNKAYTGSDEFLSQYKIKAKAKVMSIKIDTKNSLVIAILQNGLIEIFDFKLTLLHSFDISYDNLKYAKWFTENGTEYVFVLCPLQDDKVCYKLLELTDCGSGESSPIKELSSTIIEGFSFENSKLCYQFGKLYKLNQGKIYIYSLPHCQLQQVIEFPMVDKLSPGDDLISFQPVSVNRVLLTVNNVIYLLDLLHCSTLSQRELTHVKTFQLLKSAVINSEKSHNSKTIAIGISTKNGPNPTSSLEIINIDVGTNTLKDSLGKSV",
    "response#3": "MPSLSQPFRLATLPKIASLSNFSLQADYVQVADGTFNESTNNITLGISGSSISQYIINPTPKLTFDYPIPSTNIITACNAEKGQANIDGNIEASTDDEANNEKTINTQKKRNVEIWAFGLMVNKGNYTLNVITKALEDTTDTSNDHLSESDIDNKAYTGSDEFLSQYKIKAKAKVMSIKIDTKNSLVIAILQNGLIEIFDFKLTLLHSFDISYDNLKYAKWFTENGTEYVFVLCPLQDDKVCYKLLELTDCGSGESSPIKELSSTIIEGFSFENSKLCYQFGKLYKLNQGKIYIYSLPHCQLQQVIEFPMVDKLSPGDDLISFQPVSVNRVLLTVNNVIYLLDLLHCSTLSQRELTHVKTFQLLKSAVINSEKSHNSKTIAIGISTKNGPNPTSSLEIINIDVGTNTLKDSLGKSAV"
  },
  {
    "reference": "MNKFLRNVGFYLLIILVAISVIDYFSTKNTNRQEVEYTQFLQQVDKGDVSKVVIIQNTIHGTLADGTEFTTITPDAPNNDPDLYQKLSAKGIDIAAENPPEPPWWSQMFSSVIPILLLIGVWFFIMQQTQGGGGRVMSFGKSRARMSGADKIKVTFRDVAGADEAKQELEEVVEFLKHPKKFNELGARIPKGVLLFGPPGTGKTLLARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKNAPCIVFIDEIDAVGRQRGAGVGGGHDEREQTLNQLLVEMDGFAANEGIIIIAATNRPDILDPALLRPGRFDRQIVVDKPDVRGRLAILKVHTKGKPMAKDVDLDIIARRTPGFTGADLSNLVNEAALLAARREKHKVGMTEMEEAIERVIAGPERKSHVMSEEEKRLTAYHEGGHTLVGMMLKHADSVHKVTIIPRGRAGGYTLMLPKEDRNYATRSELLDRLKVAMGGRVAEEVVLKEISTGASQDIQQASRIVRSMIMQYGMSDVLGPVAYGESQNHQVFLGRDINHQRNYSEEVASEIDKEVRKYMEEAYEACRKIITENRDKLELIAQALMERETLTAKELEELLTTGSITDPDDKDEDDKPQSGTPAVTPLPVEQAEKPEAVAKETEKESEEEHEKEAEPKFNVTHYDK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Zn(2+) is required for the proper folding and stability of the protein.2. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.3. A protein that contributes to protein catabolic process by functioning in plasma membrane with ATP-dependent peptidase activity, zinc ion binding, ATP binding, metalloendopeptidase activity.4. The protein should have a high affinity for ATP.5. The protein should have a Helical domain that is long enough to span the entire membrane.6. The protein contains novel AAA+ ATPase domains that confer a unique function or activity.7. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins; Plays a role in the quality control of integral membrane proteins.8. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "MVMSEDEKAMTAYHEAGHAIVGRMVPEHDPVYKVSIIPRGRALGVTMYLPEQDKYSHSKQHLESMISSLFGGRIAEAIIYGNDKVTTGASNDIERATDIARKMVTQWGLSEKMGPMLYAEEEGEVFLGRSMARAKHMSDDTARAIDAEIKAVIDRNYERAEQILRDNIDILHTMKDALMKYETIDARQIDELLRYHRG",
    "response#2": "MVMSEEEKKLTAYHEGGHALVALHMEDSDPVHKATIIPRGRALGMVMRLPERDQLSVTRSKLKADLAVAMGGRVAEEVIFGHDKVTSGASSDISMATKMAKAMVTQWGMSDKLGPLAYQDNEEEVFLGHSVARQQTMSEDTARMIDEEVKRIVSEGYEKAQELLTKHLDELHTLAQGLLEYETLSGEEIKAQIEARARERLLSQADRERIAYHESGHAILGLLVPGADPVHRVTIVPRGQALGVTYQRPQTDRYNYPEAYLRARIIGMLGGRVAEEVIYGTRTTGAENDIEQATNLARNMVTRWGMSDKLGMVALAPHQNAFLGSSAGFGGEKPFSEETARLIDLEVQQIIHDSHAEATRLLQAHRKELDALVGALLARETLDEQEILERMTQVRHVKDE",
    "response#3": "MVMTAAQKEMTAYHEAGHALVGMELPKCDPVYKATIIPRGGALGMVMSLPEMDRLNMFKDECHQRLAMTMAGKAAEIHKYGEEAVSNGPAGDIQQASALARAMVLQWGMSDKVGNIDYSEAVQGYSGSTPGFSVSASTKELVEKEVQKFIQDGYDWALKIIKEKEDQFERLAQGLLEYETLTGAEIKRVVAEHG"
  },
  {
    "reference": "MFKKKSKYNIAIVGATGIVGESLLDIINEKNFPIDNIVPLASHRSVGSNVKFGKELITVKDXATYDFKDIDIAFFSAGAEVSQQYVPIATKAGAVVIDNTSQXRXQEDIPLVVPEVNKEAIDSHTNHSIIANPNCSTIQMLVALKPVHERFKIKNINVSTYQAVSGSGKKGXQELLEQTHSYLNQREISKSVYPSQIAFNVIPFVDSFLDNGYTKEEMKMVWETQKILDENIRVDATAVRVPVLIGHSESISXETXXXIDIDXVVNDYKTSDKXELIDDSANFEFPTAVEHGNGTDKVXVGRIRKSQXXDKILNIWVVADNVRKGAALNSVQIAEXLIKG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..2. The designed protein must possess NADP binding, protein dimerization activity, aspartate-semialdehyde dehydrogenase activity to support lysine biosynthetic process via diaminopimelate, isoleucine biosynthetic process, 'de novo' L-methionine biosynthetic process, threonine biosynthetic process under certain conditions.3. The Acyl-thioester intermediate of the protein must be engineered to enable effective catalysis of L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.4. A protein that is capable of catalyzing reactions within the L-threonine from L-aspartate: step 2/5, L-threonine biosynthesis pathway with high efficiency and specificity.5. The protein should have a high affinity for substrate, NADP(+), phosphate. The designed protein sequence is ",
    "response#1": "MAIVGATGAVGQEMMKVLAERDFKIDELICLADPREEGTQLKFKDEVITVQKVTEEAFRKADIALFAVGTEISKELAPIAVKNNCVVIDNSYAFRLEEGVPLVVPEVNAEDIKNGIPKGVKRNIIANPNCSTIQMLVALKPLHDKAKILRVVVSTYQSVSGAGKQAMDELFAQTRAIFVSDSVETKNFPKRIAFNVIPEIDVFMEDGYTKEEWKMMAETKKILDPKIKLTATCVRVPVFIGHSEAVHIEFENPISADEARDILREAPGCLVIDKRERGGYITPHEAAGEDATYISRIREDATVENGLVLWCVADNLRKGAATNSVQIAELLVARNL",
    "response#2": "MAIVGATGAVGQEMMKVLAERDFKIDELICLADPREEGTQLKFKDEVITVQKVTEEAFRKADIALFAVGTEISKELAPIAVKNNCVVIDNSYAFRLEEGVPLVVPEVNAEDIKDHQGIIANPNCSTIQMLVALKPIQDAVGIARINVSTYQSVSGAGKSAIDELAKQTADLMNGRKVTTEAFSRQIAFNAIPQIDMFMDNDYTKEEMKMVWETQKIMGDESILVNATAVRVPVFYGHGEAIHLETVNPISAEEVKALLSQAPGVVLIDDREEFPTQVGNASGKDEVFVGRVRDDITHTHGINMWVVSDNVRKGAALNSIQIAELLLNPLHKKYGIKRIVVSTYQSVTGTGKKAVDQLYNERKNVADGPMAYKYPIDLNVIPQIDVFLDNGYTKEEMKMVNETRKIMRDDNIMVTATTVRIPVVGGHSESVNVEFNSDFDIEEVKKILSEAPGVVVQDDLSAQVYPMPLWAHEKDEVFVGRIRRDDTQPNTLNLWIVSDNLRLNPLHKKYGIKRIVVSTYQSVTGTGKKAVDQLYNERKNVADGPMAYKYPIDLNVIPQIDVFLDNGYTKEEMKMVNETRKIMRDDNIMVTATTVRIPVVGGHSESVNVEFNSDFDIEEVKKILSEAPGVVVQDDLSAQVYPMPLWAHEKDEVFVGRIRRDDTQPNTLNLWIVSDNLRNPIHKEYGLKRVVVSTYQAVSGAGKAGIDELDEHIKAYMKGEEMKPSVFQYPIAFNLIPHIDVFVEHDYTREEMKMVWETRKIMHLLDLKIAATAVRVPVYRSHSESINLETYKPVTPEGVREVLSRAPGVIVQDNPQEKLYPMPLYTSHKDEVFVGRIRKDISTENGIAMWVVADQIR",
    "response#3": "MAIVGATGAVGQEMMKVLAERDFKIDELICLADPREEGTQLKFKDEVITVQKVTEEAFRKADIALFAVGTEISKELAPIAVKNNCVVIDNSYAFRLEEGVPLVVPEVNAEDIKDGLNKGIKNFIGGNCTVSCMLMALAPLHKKYKMKRVVVSTYQSVSGTGVKAVQQLENEIAGVKGEMAYPYPIGRNALPHCDVFEESGYTKEEMKLAREPQKILDDRTFSVTATAVRIPTSGGHSEAVNVEFENDFDINEIREILAATPGVIVQDNTATNTYPMPIYAHDKDEVFVGRIRRDLTQPNTLNMWIVSDNLRWGAAEPLRRMLRILEPIRQKYGLKRVIVSTYQAVSGAGAQAVEELYEQTRAIINNEPYEPKILPVRSDKKHYQIAFNAIPQIDKFQENGYTFEEMKMINETKKIMGLPDLQIAATCVRLPVATGHSESVYIEVEKDGLTVQECKEILAGAPGVELQDDIENQIYPMPADCVGKYEVFVGRIRQDLNKANGFHMWIVSDNLLLWPLHKAFGLKRAIVATYQSVSGTGAEAVRELEGQVQAHVAGSPIKAQVYPQQIAFNCLPHIDTFGPDGYTGEETKMAQESRKIMGLPELRVSATTVRVPVVRAHSIAVSAEFERPVDLAEARRAVSEFEGAELIDAPENNSYPTPLAFAEKVKCGVGRLRRDTAFDNGLSFWVSGDNY"
  },
  {
    "reference": "MSQVKRTPLYETHRALGAKMIPFGGWDMPVQYSGIIAEHNATREAAGLFDVSHMGEIFITGEPKIVLDFLELVTCNSVASLSDFQVQYNAILNENGGLVDDVTIYKFSAEKYMICSNASNYETVTAHLLKYLPASGVKVSDQSPNWHQIALQGPKANEIFSKFLGRELDSIKYYHFALLDYQGEEIIVSRTGYTGEDGFEIYSSIPFGLKLWSGLSEFGKPQGLLPCGLGARDTLRIEAKYPLYGHELNNQWTPIESGIGWIVKEKKNPYFSSGKILSQKKNGTEFKIVAFALTEAGVPRENFRVLDFQGNEIGKTTSGTFSPSLKKGIGLASIRTEKIKDGEPIQIEIREQPKQAIITTKPFIPGSIRKN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The desired protein should be able to perform (6S)-5,6,7,8-tetrahydrofolate + (R)-N(6)-(S(8)-   aminomethyldihydrolipoyl)-L-lysyl-[protein] = (6R)-5,10-methylene-   5,6,7,8-tetrahydrofolate + (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] +   NH4(+) The designed protein sequence is ",
    "response#1": "FDLYKEYGGKTIDFGGWELPVQFSSIKEEHEAVRTKAGLFDVSHMGEVEITGTDSLPFLQKLLTNDVSTLKEGGAQYTAMCYEDGGTIDDLLVYKKAANVYMLVINAANIDKDVDWMNKHIKGDVSVRNVSDEIALLALQGPKAEAILKQVADHDLAELKPFMFRDDAAVGSVQALVSRTGYTGEDGFEIYCRNEDAACIWKLLLETGKDSGLVPCGLGARDTLRFEAKLPLYGQELSKDITPIEAGIGFNISPEHEKLDWTMSNQEVFNHIRGMNPWPVAHTFLEGQRLKIYEAQLAEGEGLPGQVVVKTKKSLVIATGQGALSLIVVQPAGKPKMSIIDFLNGEVNNF",
    "response#2": "LQEVDAEVFNCISGELNRQNSGLQLIASENFVSKAVLQAQGSIFTNKYAEGYPGKRYYCGCHFADIVENLAIERLCRLFGCKFANVQPHSGSQANQGVFAALLKPGDTVIGMSLDCGGHLTHGSAPSISGKWFNAVQYQVDRDTGLIDMDEIEKLAVEHNPSLIIAGSSSYPRVIDFKRFREIADKVGAYLLADIAHYAGLIAAGEFPSPVEYAHVITSTTHKTLRGPRGAVIMTNYEDIHKKIQSSIFPGMQGGPLMHVIAAKAVAFAEALKPEFKDYAKQIIKNSKALGEVFKERGLDLVTGGTDSHMVVLDLRSKSVTGKDAVLALEKLGIICNKNAIPFDPEKPFVTSGLRFGSAAETSRGLQESEFREIGSMITKMLGDKK",
    "response#3": "FDLHLALGAKMVDFGGWDMPLHYGSQVEEHHQVRRDCGVFDVSHMNVIDVLGREAKAWLRRLLANDVDKLKTPGRALYSAMLDEQAGVIDDMIVYLTADGYRLVVNAATGAKDLAWMQSQLGDFDVQLLARSEMAMLAIQGPQARNRIAQLVSSARAELIRQLKPFEGLDDGDWFIARTGYTGEDGLEIMLPADEAQRFFNELVGAGISPIGLGARDTLRLEAGMNLYGQDIGEHVSPLASNMAAWAGAQ"
  },
  {
    "reference": "MSQTTAFPIVIVGHVDHGKSTLVGRLMHDTGAIADGAVEALQASSAKRGSVFEWSHLLDALQIERDQGITLDTTQVWFTTPKRRYVIIDAPGHKEFLKNMVTGAAAADAAILVVDARAGLAEQTRRHAYLLRLLGITQVAVAVNKIDLIDHDEARFAQVVDDVGGYLRSIGINPTALIPLSARHGDGIVAVSPQTPWYKGPSLLEALDNFAGRPSLSAQPLRLPVQDLYRRGERRIVVGRIETGRLRVGDTIRLLPGNRTVKVAGLEGWNLTVPKLSAIAGESVALAVDEDVFVERGALITEGDTSARQGHVITLRIFWLDRSALNEGDRLTLRLGTASNEVTVRRIDEALDLESLALNQSEGPGKLESGGIARVTLHSRRPIIGDRFADNPRLGRAVLARDYRLVGGGIVEDLAEVVLSAPETSVTPEERAALNGHSGGVLWLTGLSGSGKSTIAQRLLRKLTDQGRLATVLDGDTLRLGLSRDLGFSAEDRAEQVRRTAEVAKLLANSGLIVIASLISPRQADRDLAEQIIGDAFHEIFVDAALETCIARDPKGLYKRAQAGEIPNFTGISSPYEAPQDPALRLDTATLSLEDATDMLLTYAQAQFARRHENYVLGPAYI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must possess sulfate adenylyltransferase (ATP) activity, ATP binding, adenylylsulfate kinase activity to support phosphorylation, sulfate assimilation, hydrogen sulfide biosynthetic process under certain conditions.2. The protein should be able to bind ATP ligand in a variety of conditions3. We need a protein that can perform adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate   + ADP + H(+) with high efficiency.4. For general function, the protein need meet that ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity.5. We require a protein that can specifically bind to the metabolites produced in the sulfite from sulfate: step 2/3, hydrogen sulfide biosynthesis pathway. The designed protein sequence is ",
    "response#1": "TMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALENDSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADCPGHEQYTRNMVTGASTADLAVILIDARKGVLVQTRRHSYLCHLLGIRNIVLAVNKMDLIDYDQARYDAIVAEYETFAKGIGIESFTAMPISGFKGDNIQFESTLVWMSDEKLIPGRGYWLKLASQTVTATIAEPKYEVDVNTLDHLAAKTLDLNSIGVAEVTTDRPIVFEPYSDSRQLGSFILVDKITNATVAAGMIESADQFESTLVWMSDEKLIPGRGYWLKLASQTVTATIAEPKYEVDVNTLDHLAAKTLDLNSIGVAEVTTDRPIVFEPYSDSRQLGSFILVDKITNATVAAGMEVSDQFEATIVWMDEEALLPGRGYWMKLASQTVSATVAEPKYEININNVGGPGDHLAAKTLELNSIGVAEVTTDRPVVFEPYADNRALGGFILIDKITNRTVGAGMLNFALRRSENVHWQHTDVSREHHARLKGQKPAVLWFTGLSGSGKSSIANIVEKKLHALGKHSFLLDGDNVRHGLNRDLGFTDTDRVENIRRVGEVAKLMTDAGLIVLTAFISPFRAERQMVRDMMQDGEREVCVKCDFTGVYDRAKKGLYARALSGEIPNFTGIDSPYEAPESPEIVVETVGMSAEEAAERIIDFLVENGYI",
    "response#2": "MLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDGRKGILPQTKRHSYLAHLIGIRHIVLAVNKMDLVGYDRDIFDNIVRDYREFASEIGITDFTAIPISGFKGDNIKDQLRFITCGSVDDGKSTLIGRLLHDSKIIYEDQLTALAHDSARHGTNGNDVDFALLVDGLEAEREQGITIDVAHRFFTTPRRSFMVLDTPGHEQYTRNMATGASNAQLAIILVDARKGVLVQTKRHSFICSLLGIRNIVLAVNKIDLIKYKKEIFDRIVEDYIAFSASLRFTSIVPIPISARYGDNVASRSGNFEWYDGPCVLDYLESIDIQSNAVIQPFRMPVQWVNRPNLDFRGFSGLVASGTVRAGDSIRVLPSGHTSTVKSVVAYGGDLDMAQAGQSVTICLEDEIDCSRGNVISVESADQFESTLVWMSDEKLIPGRGYWLKLASQTVTATIAEPKYEVDVNTLDHLAAKTLDLNSIGVAEVTTDRPIVFEPYSDSRQLGSFILVDKITNATVAAGMIDANLVWMDPEAMKPGKVYLVKHTAQLLKGTVTSLAFRIDPETLNRQKAETLKLNEIGRVTIQLFRQIFCDEYTQNRETGSFIMIDPLTHATVAAGTILDNVTRETVADQFAAHIVWMHETPMLPGRTYQLRLGSMAVNAQVTEIKHSVNVNTLERMAAKSLDLNDVAFCNLSVDQAIPFDPYSDNRTMGAFILVDRMHNATVGCGM",
    "response#3": "MLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDGRKGILPQTKRHSYLAHLIGIRHIVLAVNKMDLVGYDRDIFDNIVRDYREFASEIGITDFTAIPISGFKGDNIFRLPVQWVCRPNQNFRGFAGPLLGGPLKVGDAVVALPSGRRSTVAGLHVGSETVDQALAGQSVIVELTEEIDVSRGDVLAEFESADQFESTLVWMSDEKLIPGRGYWLKLASQTVTATIAEPKYEVDVNTLDHLAAKTLDLNSIGVAEVTTDRPIVFEPYSDSRQLGSFILVDKITNATVAAGMEVSDQFEATIVWMDEEALLPGRGYWMKLASQTVSATVAEPKYEININNVGGPGDHLAAKTLELNSIGVAEVTTDRPVVFEPYADNRALGGFILIDKITNRTVGAGMLRFALRRSQNVADQFAAHIVWMHETPMLPGRTYQLRLGSMAVNAQVTEIKHSVNVNTLERMAAKSLDLNDVAFCNLSVDQAIPFDPYSDNRTMGAFILVDRMHNATVGCGMIRHSLRRAQNVHKQALDITQFESTLVWMSDEKLIPGRGYWLKLASQTVTATIAEPKYEVDVNTLDHLAAKTLDLNSIGVAEVTTDRPIVFEPYSDSRQLGSFILVDKITNATVAAGMIRHSLRRAQNVHKHSMDITP"
  },
  {
    "reference": "MSKVLFINASPVANEASFSYQLAKTFESEYLKLNPSDQVSWLDLNELDKGSMTLTSKNSKEHFKDENVDPLINQIKSVDKLVVIAPMTNFNYPATLKNWLDKICVANKTFSYKYSKKGGSIGLMDHLKVMIINTQGAPEGWYAFADVTVLLKGVFEFIGAMVDSIKVAGTKVEYLNKQPKEIVEPNLALIKEKAKNF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit FMN binding.2. The FMN binding site should be located in a region of the protein that is accessible to the ligand.3. For general function, the protein need meet that Catalyzes the reductive cleavage of the azo bond in aromatic azo compounds to the corresponding amines. The designed protein sequence is ",
    "response#1": "TLLQIKSSLFGDNGNSSQLANEFVERWKTQNPNGEVVIRDFAKEDVPHLDATRVQALFTPAEQRTAEQQAVVEYSDKIIAEIQAADAIVLGVPLYNFGIPSTLKAYFDHIARAGVTFKYTETGPVGLLTDKPVYILAARGGIYKGQPSDTQSRYLVDFLNFVGLKDIHFIYAEGLNMGQKDQAFASAKHEIEAN",
    "response#2": "MRLLHLDSSILTDTSVSRQLTAQVVQDLHAAIDDLHATYRDLAAVPIAHLSGAIAAGFRPLPQPASDAALVAEHALSEQLVDEFLASDIVVIGAPMYNFSVPTQLKAWIDRIAQPGRTFRYTANGPEGLAGGKQLIVASSRGGMYTQGPMASLDFQEAYLTATFGFLGVRDVHFVRAENQSRGPEPAAAALISAQAL",
    "response#3": "MRLLHLDSSILTDTSVSRQLTAQVVQDLHAAIDDLHATYRDLAAVPIAHLSGAIAAGFRPLPQPASDAALVAEHALSEQLVDEFLASDIVVIGAPMYNFSVPTQLKAWIDRIAQPGRTFRYTANGPEGLAGGKQLIVASSRGGMYTQGPMASLDFQEAYLTATFGFLGVRDVHFVRAENQSRGPEPAAAALISAQALIS"
  },
  {
    "reference": "MPKSKVNSAEENYSSSSSADRRTPVKLNSSGTPRWYIVLMLALMLLGLAWLVVNYIAGPEIPFMRDLNAWNYLIGFALLIVGLLMTMGWK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should contain a Helical membrane-spanning region.2. The protein must exhibit the following characteristics: Involved in cell division..3. The protein should be optimized for stability and expression in plasma membrane where it will participate in cell cycle. The designed protein sequence is ",
    "response#1": "IRNYFGSSIGLYFGFIEFYTKALIFPSLFGILQYVFDLNISLVCSFYVVWTTIFLELWKRKCAGYSYRWGTIEMSSLDKPRSAYTGQLKPDPITGKMTLHYPMRYTYLQMYCISYPVVLGCVVAAGWFALYQFQIEAEVLADFGPDSWLLYVPVIVQSVLIAIFSWAYEKLATFLTNLENHRTRSQYDRHRVNKLMLFEIVNNFFSQFYIAFVLHDLRQLKYQLMMQLLVFQLLCIAQEIGIPLLAVLRQKYAEFRHREVAEEKLRSISDLPRYEQSFYESGLDEYHSTYEDYLQVCIQFGFVVLFAAVAPFAAIGALLNNVFAVHIDMWKLCNIFKRPFARRAKNIGAWQLAFELLSVMSLLSNCGLLFLQPNVKDFFSHWLPSVPDLSFVIFEHLLLGLKFLIHKVIHERPRWVRIY",
    "response#2": "VLMVGPPGTGKTLLAKAIAGEAKVPFFSISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFEGGEGVIVIAATNRPDVLDPALLRPGRFDRQVVFTP",
    "response#3": "IPAEVVLPLAGYWVSKGDMTLAGVVLAGSLGGVAGPLTLYWIGRYGGRPFLERFGKYLFIKPEALDKSDNFFKKHGGFVAFSGRFLPGIRTLISIPCGIAKMNVWVFSLYTFIAMLPITFVYVYLGS"
  },
  {
    "reference": "MAASGPGCRSWCLCPEVPSATFFTALLSLLVSGPRLFLLQQPLAPSGLTLKSEALRNWQVYRLVTYIFVYENPISLLCGAIIIWRFAGNFERTVGTVRHCFFTVIFAIFSAIIFLSFEAVSSLSKLGEVEDARGFTPVAFAMLGVTTVRSRMRRALVFGMVVPSVLVPWLLLGASWLIPQTSFLSNVCGLSIGLAYGLTYCYSIDLSERVALKLDQTFPFSLMRRISVFKYVSGSSAERRAAQSRKLNPVPGSYPTQSCHPHLSPSHPVSQTQHASGQKLASWPSCTPGHMPTLPPYQPASGLCYVQNHFGPNPTSSSVYPASAGTSLGIQPPTPVNSPGTVYSGALGTPGAAGSKESSRVPMP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. Target a Polar residues compositional bias in the protein's composition for improved properties.3. The designed protein should have serine-type endopeptidase activity, misfolded protein binding, ubiquitin-specific protease binding within Golgi apparatus, Hrd1p ubiquitin ligase ERAD-L complex, endoplasmic reticulum membrane, Golgi membrane to regulate endoplasmic reticulum unfolded protein response, ubiquitin-dependent ERAD pathway. The designed protein sequence is ",
    "response#1": "QMCIRDFVSEGELVVINIDTDGSLNDGNVLNLYVHDSNGNEYRRLKNFVGEQRIAFTAPATTSFDVCFENTLDSNRGNRNAKRAIELDIEC",
    "response#2": "QMCIRDFVSEGELVVINIDTDGSLNDGNVLNLYVHDSNGNEYRRLKNFVGEQRIAFTAPATTSFDVCFENTLDSNRGNRNAKRAIELDIEC",
    "response#3": "ANPPYSLFYKSTLGHTISRETQNLQVPYFVDKNFDKAYRGASLRDLEKTIEKDYIDYIQTSCWKEKQQKSELTNLAGLYRDERLKQKAESLKLENCEKC"
  },
  {
    "reference": "MPVSLSTALRVVGTSLFALAVLGGILAAYVTGYQFIHTEKHYLSFGLYGAILGLHLFIQSLFAFLEHRRMRAERQPVRLGRSVALCIAAYQEDPDYLKKCLLSVKRIAFPDLKVVMVVDGNGPDDTYMLDIFHDVMGSERAGSYVWRSNFHARGEGETEAGLQEGLAHVQALVRSTTYSCILQKWGGKREVMYTAFRALGDSVDYIQVCDSDTVLDPACTAEMLRILEADPRVGGVGGDVQVWRRGGDGTHGGTALALVALTPSPPQILNKYDSWISFLSSVRYWMAFNVERACQSYFGCVQCISGPLGMYRNALLQQFLEDWYHQTFLGTKCSFGDDRHLTNRVLSLGYQTKYTARSRCLTETPTRYLRWLNQQTRWSKSYFREWLYNALWFHKHHLWMTYESVVTGFFPFFLIATVIQLFYRGRIWNILLFLLTVQLVGIIKATYACFLRGSAEMIFVSLYALLYMSSLLPAKMFAIATINKSGWGTSGRRTIVVNFVGLLPVSVWAAVLLGGLAYTAYSQDLLSDTEVAFLISGAVLYACYWVALLTLYLAMVARRCGKRKEQCGLVFAEV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be able to modulate extracellular polysaccharide biosynthetic process in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "MKFENIHPEEQRYNFEEVARIKEFAIKNDMKLRGHTFVWHNQTPGWVFLDKNGEEASKELVIERLREHIKTLCERYKDVVYAWDVVNEAVEDKTEKLLRESNWRKIIGDDYIKIAFEIAREYAGDAKLFYNDYNNEMPYKLEKTYKVLKELLERGTPIDGIGIQAHWNIWDKNLVSNLKKAIEVYASLGLEIHITELDISVFEFEDKRTDLFEPTPEMLELQAKVYEDVFAVFREYKDVITSVTLWGISDRHTWKDNFPVKGRKDWPLLFDVNGKPKEALYRILRQ",
    "response#2": "EMLNLSLAKTYKDYFKIGAAVTAKDLEGVHRDILLKHFNSLTPENAMKFENIHPEEQRYNFEEVARIKEFAIKNDMKLRGHTFVWHNQTPGWVFLDKNGEEASKELVIERLREHIKTLCERYKDVVYAWDVVNEAVEDKTEKLLRESNWRKIIGDDYIKIAFEIAREYAGDAKLFYNDYNNEMPYKLEKTYKVLKELLERGTPIDGIGIQAHWNIWDKNLVSNLKKAIEVYASLGLEIHITELDISVFEFEDKRTDLFEPTPEMLELQAKVYEDVFAVFREYKDVITSVTLWGISDRHTWKDNFPVKGRKDWPLLFDVNGKPKEALYRILRP",
    "response#3": "MKFENIHPEEQRYNFEEVARIKEFAIKNDMKLRGHTFVWHNQTPGWVFLDKNGEEASKELVIERLREHIKTLCERYKDVVYAWDVVNEAVEDKTEKLLRESNWRKIIGDDYIKIAFEIAREYAGDAKLFYNDYNNEMPYKLEKTYKVLKELLERGTPIDGIGIQAHWNIWDKNLVSNLKKAIEVYASLGLEIHITELDISVFEFEDKRTDLFEPTPEMLELQAKVYEDVFAVFREYKDVITSVTLWGISDRHTWKDNFPVKGRKDWPLLFDVNGKPKEALYRILRG"
  },
  {
    "reference": "GILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSEYDFPGDDVPVIAGSALKALEGDEKYEEKILELMQAVDDYIPTPERDSDKPFMMPVEDVFSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIGALLRGVAREDVQRGQVLAAPGSITPHTKFKAEVYVLSKDEGGRHTPFFSNYRPQFYFRTTDVTGVVQLPEGTEMVMPGDNVEMTVELIAPIAIED",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis.2. The designed protein should contain one or more tr-type G domains that are essential for its function.3. A protein that localizes to cytoplasm and can perform GTP binding, GTPase activity with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "KNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPSIVVFLNKADQVDDEELLELVELEVQETLSEYEYPGEEIPIISGSALLALEALTENPELDSANNEWVQKIYHLMDSVDDYIPAPRG",
    "response#2": "NFSIIAHIDHGKSTLSDRLIQVCGGLTDREMAEQVLDSMDLERERGITIKAQSVTLDYTAKNGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALEMDLEVVPVLNKIDLPQAEPERVAAEIEDIVGIEATDAVRCSAKTGVGIGEVLETI",
    "response#3": "KNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPSIVVFLNKADQVDDEELLELVELEVQETLSEYEYPGEEIPIISGSALLALEALTENPELDSANNEWVQKIYHLMDSVDDYIPEQ"
  },
  {
    "reference": "MVHDFDPFALRISGDFGIRWYGLSYMMGFICAYILITWLAKRQRAGLSVQMVGDFITYAAIGTLVGGRLGYVLFYGPDLFLKFKSEFPYWGVLAVNEGGMASHGGIIGIVIACLLYARKYSVNSLYLLDLVAVTGPIGVFFGRIANFINGELVGRPCDPTYPLAVKFPQDIVSWPGQEAAKLTELVPVVEKVGVGREQWLELVDKFRFDMAAREQLYATLNKVIESIQDGNTAAKEAIAPLLTPRYPSQLFAAFGEGLLIFMFLFFLWRKPRKPGFIAACFVLIYAVVRVVDEHFRMPDAHIGFQALGLTRGQWLSLAMFVVGLILMVVWTRAASLKIPGWQRGHSIKLNRK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) binding site should be located in a region of the protein that is accessible to the ligand.2. The designed protein should have phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity to facilitate lipoprotein biosynthetic process.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.4. Ideal protein should have a binding affinity for key enzymes in the Protein modification pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "GQLLN",
    "response#2": "MNTSTDNS",
    "response#3": "ERLS"
  },
  {
    "reference": "MATIFFAEPELVIGHGRKVLFLNPGDLQIFKEIELPPDLTTCGLKTVEPVPAPGHPASSSKQQPAALKEATGSVKVEVSIQNVTYSPDRQLLALTTAGQKAVLLYKSRPENAQLLSIRPLARASSALRFCSDGSSVLVTDKTGDCYQYDCVEVDASPRLLLGHLSIVYDVLWSEDQQYIITCDRDDKIRVTNYPATFDIHSYCLGHKEFVSGLAMLTEQHIISASGDKTLRVWNYTCGKELLLHELPAPAVRMLVRQLEPEKTYEVAVLFYDYVDAIGVYRLEQTTTESWSITSTQLVRAEAGTWNICNFALTDDRIYVTGAENERLTLRVYDSRNGERASGLPEGWLKMVLDNLDVAAFMPEDLSVWFKKRFDNVSEYLERKKRRIEEQKQQKCG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit; Required during gametogenesis..2. A protein that is capable of catalyzing reactions within the tRNA modification pathway with high efficiency and specificity.3. The protein should be optimized for stability and expression in nucleus, female germ cell nucleus, male germ cell nucleus where it will participate in tRNA methylation, RNA (guanine-N7)-methylation, spermatogenesis. The designed protein sequence is ",
    "response#1": "CEQRFLPCEIGCVRYSLQEGIMAVFHSFINPGEIPRGFRFHCQAASDSSHKIPISNFEFGHDQATVLQNLYKFIHPNPGNWPPVYCKSDDRARVNWCLKRMARELEISQDIELLTVEDLVIGIYQEKFLKEPSKTWVRSLLDVAMWDYSSNTRCKWHEENDILFCALAVCKKIAYCISNSLATLFGIQLTGAHVPLK",
    "response#2": "YSRIGHIAHLNLRDHLLPYKQLIGQVLRDKLPNCRTVVNKASSIDNTYRNFQLELICGDPDYQVETKENGVPFEFDFSKVYWNPRLSTEHERIVKMLKSDDVLYDVFAGVGPFSIPAAKKRCHVLANDLNPESFRWLQHNAKRNKCLPNIKMSNKDGRQFIVEELREDLLKRLCTTDTTTYGIHITMNLPAMAVEFLDAFRGLYSADELAQLPTNVCYPTVHVYSFAKGENTKELVRQLVESNLGASLDENLLQGINFVRNVAPNKDMYRVSFKLSD",
    "response#3": "FEMVGHIAHLNLRDEHLPYKKLIAKVVLDKNKPKIQTVVNKTDAIHNDYRTMQLEVLAGNRSLVTTVIENGMRFQVDLATVYWNSRLATERQRLLNCFTRNDVVCDVFSGVGPIAISAAKKVKRVYANDLNPYAIEYLESNSVLNKLERKIKVFNMDGRRFINAMFTSDKAESITQVVMNLPNDAAEFLDAFRGIFRKKSRDKQLKLPMIHVYGFSKAQDPEFDFHQRIRIALSEVAVDVEMHRVRLVAPGKWMLRASFILPR"
  },
  {
    "reference": "MTGKQIVLGVTGSIAAYKAVSLLRTLIREGAVVHVVMTQSATKFVTPLTFEVLSGHPVSTHIFDTHQEMKHLSLPAQADAIVIAPATANCLAKTALGLGDDLLSTMLLTSRCPLVVAPAMDGGMWTHPSVLDHVQTLRARGTIVVNPEIGPLASGQIGQGRLAEEERILKALQAALVPQRDWQGHRVLVSAGPTQEPIDPVRFISNRSSGKMGYAIAEAAQARGAQVVLVTGPTALPIPKGIEVVSVATAEDMLKALTTRLPWSTTVIMAAAVADFRPIHPAFQKLKKQGRTGQTLDLEPTTDILAALSAQRTTQLMVGFAAETGDLIAHAKAKLEAKGLDLIVANDVTTEGAGFGSDQNAATLIDRQGNMIELPLMPKRALADAILNRAQELLLTRQSSHSPKPVVRER",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. We need a protein that can perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2 with high efficiency.2. A protein with FMN binding, metal ion binding, phosphopantothenoylcysteine decarboxylase activity, phosphopantothenate--cysteine ligase activity functions is required to enable coenzyme A biosynthetic process, pantothenate catabolic process.3. The designed protein have Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains with specific sequence motifs that are crucial for its activity.4. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.5. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.6. A protein should be able to manipulate the activity of enzymes in the Cofactor biosynthesis, coenzyme A biosynthesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "MTVIVGITGGIAAYKAVGVVRDLVKRGHDVHVVPTEGALRFVGLPTLEALSRNPVTTSVWDDVAEVRHVALGRRADLVVIAPATADSLARMAHGLASDLLGTTLLATEAPVVVAPAMHPQMWEHPATRANVATLRDRGVHLVGPVVGALTGDDAGIGRMAEPEDIVAAALALLRGDGPRPLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHILDAEGAVVAEADGTKADVATAVLDLVATR",
    "response#2": "MTVIVGITGGIAAYKAVGVVRDLVKRGHDVHVVPTEGALRFVGLPTLEALSRNPVTTSVWDDVAEVRHVALGRRADLVVIAPATADSLARMAHGLASDLLGTTLLATEAPVVVAPAMHPQMWEHPATRANVATLRDRGVHLVGPVVGALTGDDAGIGRMAEPEDIVAAALRLAAAVLGRALAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHVLGADGAVTEVASGSKAAVAAAVWDRVAALLAG",
    "response#3": "MTVIVGITGGIAAYKAVGVVRDLVKRGHDVHVVPTEGALRFVGLPTLEALSRNPVTTSVWDDVAEVRHVALGRRADLVVIAPATADSLARMAHGLASDLLGTTLLATEAPVVVAPAMHPQMWEHPATRANVATLRDRGVHLVGPVVGALTGDDAGIGRMAEPEDIVAAALRVTGGTAGALAGVRVVVSAGGTREPFDPVRFVGNRSSGRQGIAIAADAVRRGASVTLVAANVEGSLTAGLDATVVPVGSALELAEAVHAAATEADVVVMTAAVADYRPAEVLTEKLKKDAQGERMTLELVRNPDVLADLVAARRTGQIIVGFAAETEPDRDARIELGRAKISRKPADLLVVNHVGWSAGFEREENAIEVLAADGTVEELARGDKTALAHAVWDAVVALLD"
  },
  {
    "reference": "MGNKWSKSSKEGWHQVREKMKQTPPATEGVGAVSRDLDKHGAVTSTNVNNADSVWLRAQEEEDEGVGFPVKPQVPLRPMTFKGAFDLSFFLKEKGGLDGLIYSKRRQEILDLWVYHTQGFFPDWHNYTPGPGIRYPLCFGWCFKLVPVDPKEVEEDNKGENNCLLHPMSQHGIEDDEREVLMWKFDSALARRHIARELHPEYYKDC",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike.2. The protein design must feature the Diacidic; necessary for CD4 internalization, PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Dileucine internalization motif; necessary for CD4 internalization motif.3. A protein that can perform SH3 domain binding, GTP binding in extracellular region, virion component, host cell Golgi membrane, membrane, host cell plasma membrane to support suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host autophagy, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II. The designed protein sequence is ",
    "response#1": "MGNKWSKSWPAVRERMRRTEPAAEGVGAVSRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVEPEKIEEATEGENNCLLHPMSLHGMEDEDREVLMWKFDSRLAFHHMARELHPEYYKDC",
    "response#2": "MGGKWSKRSRAGWPAVRERMRRAEPTAEGVGAASRDLDKHGALTSSNTAANNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLVYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRYPLTFGWCFKLVPVEPEKIEEANEGENNCLLHPMSQHGMDDPEREVLVWKFDSRLAFHHVAREKHPEYYKNC",
    "response#3": "MGGKWSKSSIVGWPAIRERIRRTEPAAEGVGAVSRDLERHGAITSSNTAANNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLVYSQKRQEILDLWVYHTQGFFPDWQNYTPGPGVRYPLTFGWCFKLVPVEPEKVEEANEGENNSLLHPMSLHGMEDPEKEVLVWQFDSRLAFHHVARELHPEYYKNC"
  },
  {
    "reference": "MIQFQPVLGSLKSYEAGKPIELVVREFGIDPDQIIKLGSNENPFGCAQPVVEAVQKAASKMPYYPDDSYLELKTALASKFDLTPDRIILGNGSDQVLDFACRCVLGPGDSILINRITFAMYRIYALQCGAKVHSTETVLHDLNAFLDLAKSIRPKIIFLCTPSNPIGDALFKSDVYDFLRQIPSNTLVVIDAAYMEFGKKKDSNTFISAKEVTDLFPNVFYTGTFSKAYGLGGMRIGYGIGSKELISNLYKMRPPFNVANLSALAATEALKNESHVESYLDNNLKEMKRYEKFAAEQSVEFIDSYANFITFFARKRGKSSTEISQSLLKQGIILRNLRSYDLNAIRITIGKPQQNDRVLTALNQEFS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that is capable of catalyzing reactions within the Amino-acid biosynthesis, L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9, L-histidine biosynthesis pathway with high efficiency and specificity.2. The presence of pyridoxal 5'-phosphate is necessary for the protein to function in the desired environment.3. The protein should have a fast turnover rate for 2-oxoglutarate + L-histidinol phosphate = 3-(imidazol-4-yl)-2-   oxopropyl phosphate + L-glutamate.4. A protein with histidinol-phosphate transaminase activity, pyridoxal phosphate binding functions is required to enable histidine biosynthetic process. The designed protein sequence is ",
    "response#1": "LRGGNLNRYPACQPAGVINGYAAYAGVQPEQVVVCRGADEGIELLIRAFCEPGADAILFCPPTYGMYSVSAETFGIARRTVEARQDWQLDLQAIRARLDGVKLVYICSPNNPTGNLIDLETLRAMLEMTHGRALLVVDEAYIDFCPQASVVNWLAEYPHLVILRTLSKAFALAGLRCGFVLANVEVIQLLLKVIAPYPLALPVADIAEQALSDEGLRQMRTRVAEINTNRETLAHGLADCPCICAVYPSVSNYLLVRCDPAYRVFKTLWDQGTILRDQSKQPGLADCLRITIGTLAECQSVIAG",
    "response#2": "LRGGNLNRYPACQPAGVINGYAAYAGVQPEQVVVCRGADEGIELLIRAFCEPGADAILFCPPTYGMYSVSAETFGIARRTVEARQDWQLDLQAIRARLDGVKLVYICSPNNPTGNLIDLETLRAMLEMTHGRALLVVDEAYIDFCPQASVVNWLAEYPHLVILRTLSKAFALAGLRCGFVLANVEVIQLLLKVIAPYPLALPVADIAEQALSDEGLRQMRTRVAEINTNRETLAHGLADCPCICAVYPSVSNYLLVRCDPAYRVFKTLWDQGTILRDQSKQPGLADCLRITIGTLAECQSVIAG",
    "response#3": "LRGGNLNRYPACQPAGVINGYAAYAGVQPEQVVVCRGADEGIELLIRAFCEPGADAILFCPPTYGMYSVSAETFGIARRTVEARQDWQLDLQAIRARLDGVKLVYICSPNNPTGNLIDLETLRAMLEMTHGRALLVVDEAYIDFCPQASVVNWLAEYPHLVILRTLSKAFALAGLRCGFVLANVEVIQLLLKVIAPYPLALPVADIAEQALSDEGLRQMRTRVAEINTNRETLAHGLADCPCICAVYPSVSNYLLVRCDPAYRVFKTLWDQGTILRDQSKQPGLADCLRITIGTLAECQSVIAG"
  },
  {
    "reference": "MKGGEGDAGEQAPLNPEGESPAGSATYREFVHRGYLDLMGASQHSLRALSWRRLYLSRAKLKASSRTSALLSGFAMVAMVEVQLESDHEYPPGLLVAFSACTTVLVAVHLFALMVSTCLLPHIEAVSNIHNLNSVHQSPHQRLHRYVELAWGFSTALGTFLFLAEVVLVGWVKFVPIGAPLDTPTPMVPTSRVPGTLAPVATSLSPASNLPRSSASAAPSQAEPACPPRQACGGGGAHGPGWQAAMASTAIMVPVGLVFVAFALHFYRSLVAHKTDRYKQELEELNRLQGELQAV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should emphasize Polar residues compositional bias.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. For general function, the protein need meet that Ca(2+) release-activated Ca(2+)-like (CRAC-like) channel subunit which mediates Ca(2+) influx and increase in Ca(2+)-selective current by synergy with the Ca(2+) sensor, STIM1.4. The designed protein should have store-operated calcium channel activity within membrane to regulate store-operated calcium entry. The designed protein sequence is ",
    "response#1": "CICETCDRERLNDSLQYSLKRINTFRALASPAWMSLTSPDPILSAFKLSWDLQRLAFEEHEFR",
    "response#2": "CICETCDRERLNDSLQYSLKRINTFRALASPAWMSLTSPDPILSAFKLSWDLQRLAFEEHEFH",
    "response#3": "CICETCDRERLNDSLQYSLKRINTFRALASPAWMSLTSPDPILSAFKLSWDLQRLAFEEHEFM"
  },
  {
    "reference": "MSNSWWEITILCHPHLEESITWRLDKFGCSGTSREIKGKSYIIQAYAPQIQYQSLDISALSLWLQQDALVLNLPQPLTRWKLIDEEDWASSWKEHWEPTEVGDRFIIYPAWLTPPEQTDKLILRLDPGVAFGTGTHPTTQLCLESLEMRLSKSPENVVIADIGSGSGILSIGAILLGAKKAYAVDIDPLAVKAARENRHLNQIHPDHLVINQGSLTELLELVSDGVDGIVCNILAEVIIELIPQFSKLARPNTWGILSGILLEQSQAIADTLEQEGWIVAALWKRKQWCCFQIRKAPEN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Methylates ribosomal protein L11.2. The protein should be designed to catalyze the following reaction L-lysyl-[protein] + 3 S-adenosyl-L-methionine = 3 H(+) +   N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-   homocysteine.3. The protein can be expressed in cytoplasm and perform histone lysine N-methyltransferase activity without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "ASVLDIGTGNGVFLVELVKHGFSNITGIDYSPSAIKLSASILEKEGLSNINLKVEDFLNPSTKLSGFHVCVDKGTYDAISLNPDNAIEKRKQYVMSLSRVLEVKGFFLITSCNWTKAELLDAFSEGFER",
    "response#2": "ASVLDIGTGNGVFLVELVKHGFSNITGIDYSPSAIKLSASILEKEGLSNINLKVEDFLNPSTKLSGFHVCVDKGTYDAISLNPDNAIEKRKQYVMSLSRVLEVKGFFLITSCNWTKAELLDAFSEGFEL",
    "response#3": "ASVLDIGTGNGVFLVELVKHGFSNITGIDYSPSAIKLSASILEKEGLSNINLKVEDFLNPSTKLSGFHVCVDKGTYDAISLNPDNAIEKRKQYVMSLSRVLEVKGFFLITSCNWTKAELLDAFSEGFEL"
  },
  {
    "reference": "MEPRAVADALETGEEDVVMEALRAYNRENSQSFTFDDAQQEDRKRLAKLLVSVLEQGLPPSRRVIWLQSIRILSRDRSCLDSFTSRRSLQALACYAGISASQGSVPEPLNMDVVLESLKCLCNLVLSSPVAQALAAEAGLVVRLAERVGLCRQSSFPHDVQFFDLRLLFLLTALRTDVRQQLFQELQGVRLLTRALELTLGMTEGERHPELLPPQETERAMEILKVLFNITFDSIKREVDEEDAALYRHLGTLLRHCVMLAAAGDRTEELHGHAVNLLGNLPVKCLDVLLTLEPHEGSLEFLGVNMDVIRVLLSFMEKRLHQTHRLKESVAPVLSVLTECARMHRPARKFLKAQVLPPLRDVRTRPEVGELLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLAARGLMAGGRPEGQYSEDEDTDTDEYKEAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAMKLVNMFDKLSRHRVIQPMGMSPRGQLTSLQDAMCETMEGQLSSDPDSDPD",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should be able to enhance the efficiency of visual learning. The designed protein sequence is ",
    "response#1": "LDCSNIKVDGVMWNFGKLGGAHSISETASSHEGYNTTYTLDICKPLTKTLCKKGAFVCAVRNATDINGIERTMDVIDIAGNFVLNSGRTLDPIFTRLKKEDPKTEGLKMELHGGKHKFGNLLKRQKAVITLLCDRERTGLEGLESPKPDGDKKKDGEKKDDDKKDNKDKEGKSKRDGEENKRSLIFKSYNEEEGTLDLEWKTKYACEL",
    "response#2": "KEQFFHEEECGDLVTRAVLVDMEPKVVSKTLSMAGRSGKWKYDISSQFSQKQGCGNNWANGYCVHGPKHQDIVMDLVRKQVEKCDRLGGFFTIMSMAGGTGSGMGTFLTRCLRDAYPDSLLLNQVIWPYGTGEVIVQNYNSILTLSHLYRSSDALLVHENDIIHKVCSQLMNIKQISFRDVNKVIAHQLGSIFQPVYTSC",
    "response#3": "LDCSNIKVDGVMWNFGKLGGAHSISETASSHEGYNTTYTLDICKPLTKTLCKKGAFVCAVRNATDINGIERTMDVIDIAGNFVLNSGRTLDPIFTRLKKEDPKTEGLKMELHGGKHKFGNLLKRQKAVITLLCDRERTGLEGLESPKPDGDKKKDGEKKDDDKKDNKDKEGKSKRDGEENKRSLIFKSYNEEEGTLDLEWKTKYACEM"
  },
  {
    "reference": "MTVRLLIASNNQDKIREFREIFADLPLTLTHPAAEGLTLEPEETGQTFTENAIIKAMAFARATGLLTLADDSGLEVDALGGEPGVHSARYCGTAKDDHAGRYRLVLQKLAESGVPWEQRTARYRCVVAVAVGDRLVGTVEGTVEGYIAYQPKGSGGFGYDPVFYVPEYNCTMAELTPAQKHAISHRGRAARAAIPLLRQALGL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP The designed protein sequence is ",
    "response#1": "GFSS",
    "response#2": "MGKLSA",
    "response#3": "MARR"
  },
  {
    "reference": "MAPQMSQKEADIAMMLAAGCHLGTKNVDFQMERYVWKRRADGIHIINLGKTWDKLMLAARVIVAVENPQDVIAQAARPYGQRAVLKFAQYTGAKALAGRHTPGTYTNQKDAVFAEPRVLVVTDPRTDAQPISETAFVNIPTIAFCDTDSPLKNVDIAIPANNKAKQSIGCLYYLLARMVLQMRGTISPANPWDVMVDLFFYRDPEELEAKEQEEEETAAAAAAPAADAGYNAVADAAYGAESWDDQAGAGFGAQAGNWSEAPAPTGWDAQQGGDFGQGFGAMPPQGY",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be optimized for structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "MK",
    "response#2": "MPIAAEPRTEFGKGGARRTRRAGKVPAVLYGHGKPPRHIALPAHDLLHAFKTDAGTNVLLTLELAEGTELALAKDVQRHPIKGSFEHIDD",
    "response#3": "MNIAAEPRTEFGKGGARRTRRAGKVPAVLYGHGKPPRHIALPAHDLLHAFKTDAGTNVLLTLELAEGTELALAKDVQRHPIKGSFEHIDD"
  },
  {
    "reference": "MKIVSRXEMLMAEQAIYDQGVSNEALIWRVGVELSDHIXXIAEXSGLQXVAILVGPGNNGADGLVAAVNLAEKGFHVHVYFCTDKDRQNADLIXRVRISGGXTXLAKEVPXXFQXDIAQFDIVVXAILGISKPRKVEGVLAEVLHHVREALYSSKNSVVIAVDVPSGMDADTGEVDDLTIPANYTLAVGYPKRGFFLSPGCNYVGVIQXVDIGLYSEPLXALLXNMTSVEDFXKDRESFRLAVDSHKGSKGHVLAIVGSAQYPGAAILTGXAAYRSGCGLVTLMSPXSIFPVIGAGLPEAIHLTYPDLISDEATXQATELVSNLDLNKYDSIVLGCGLSNGHLXSVFVNAVLDYVGKSGDSVPMVVDAGAXNCLADSGSWXXKLDRSLVLTPHAGEMSRLVKSSVEYVQQNRLEVASAIATKVNQITVLKGAFTVVADVDGSIYVNPHANPVLGTAGSGDVLSGLIASLLAQGLPELLAVKLAVYVHGQAGQELRLRVGDXGVXATDIVNDXPKVLNELVM",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration2. The designed protein must have at least one YjeF N-terminal domain.3. The protein design must be able to perform ATP binding in order to contribute to nicotinamide nucleotide metabolic process.4. The AMP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MRIEDVYIWDINAKWLGITPYQLMENAGAGVAKIIEERVGEGLKIAVFSGTGNNGGDGFVAARHLSFENNVTLFLVGDEAKIGSEEARHNWEILRKLDFVKIIVLKDSTYIKPLDLSGFDVIVDALLGAGTRGEPREPIRSAIEKINKYAGRVKIVSVDLPSGYPSDVRVKCDFAVTFQWDKEEYGGFERVISKIGYADTHKFRRGAVAVLAGGVAGVGAPRLSARAALRVGAGLATLLCEPEALPVHAARGPDALMQRAVADRGALEAFLRDRRVSAVLAGPALGLDRRADDLLRAVIADDRPAVLDADALTALAGLPDGGVRLLGGRGGDTVLTPHEGEFQRLFGEEPAIAAEPSKLERARKAAALSGAVIVYKGADTVVAAPDGRGAINVTGSVALATAGSGDVLAGLVAGLLAQGMPAFEAGCAAVWLHGLAGEKLGMGLIADDLPEAIS",
    "response#2": "MQELDRRTIAEARIPGTTLMERAGSGVVSCLEQTFGSTRGKIVTILCGKGNNGGDGCVVARLLHRRQAKVLVLAMAPVSEMSRDAATMYRQFVRSAGKSAVRPYTSKSQTQSLLRDSDILVDALLGTGLTSEVTGRYGEAIDSINEASRPVVAVDLPSGLHADTGAILGRAVQASLTVTFGLPKFGLYQNHGIDLAGLVTIVDIGIPQAYIPEITAGGTGDVLTGISHKGSYGHLAVITGNKVGAGQLAALAAFSFGCGLVTTVSALEKPEVGQPLHIMHSEYIPKSTNAIALGMGLGRDELPSQLSSWLTAPVNKVIDADLFYWGKLAEFFEHPLVLTPHPKEFCALLKSTKLASITVKELQQDRFYYLRLFCDKYPKVTLLLKGANCLIGQGQSIYINDNGSAILSKGGSGDVLSGLIASLLAQGYSPLDAAIHGSLAHTQAAQELTINNYAMTPQDLIAQLADTHKFRRGAVAVLAGGVAGVGAPRLSARAALRVGAGLATLLCEPEALPVHAARGPDALMQRAVADRGALEAFLRDRRVSAVLAGPALGLDRRADDLLRAVIADDRPAVLDADALTALAGLPDGGVRLLGGRGGDTVLTPHEGEFQRLFGEEPAIAAEPSKLERARKAAALSGAVIVYKGADTVVAAPDGRGAINVTGSVALATAGSGDVLAGLVAGLLAQGMPAFEAGCAAVWLHGLAGEKLGMGLIADDLPEAIAA",
    "response#3": "AVLAGGVAGVGAPRLSARAALRVGAGLATLLCEPEALPVHAARGPDALMQRAVADRGALEAFLRDRRVSAVLAGPALGLDRRADDLLRAVIADDRPAVLDADALTALAGLPDGGVRLLGGRGGDTVLTPHEGEFQRLFGEEPAIAAEPSKLERARKAAALSGAVIVYKGADTVVAAPDGRGAINVTGSVALATAGSGDVLAGLVAGLLAQGMPAFEAGCAAVWLHGLAGEKQ"
  },
  {
    "reference": "MQGKHIVLGITGSIAAYKAAYLVRGFIKKGAEVKIIMTENAKQFITPLTMATLSQNPIAVEFFDPTNGEWHSHVKLGLWADVFLVAPATANTLGKMANGIADNLLTTTFLSAKCPTVVVPAMDVDMYSHPAMRNNLNALRNMGVGILEPETGELASGLLGKGRMPEPETILEYMESFFNKEQSMSKKKVLITAGPTYEKIDPVRFIGNYSSGKMGYALADECAKQGAEVCLISGPVSIKPTQNNIKVISVESANEMYTAAIEQFQQADIAILCAAVADFTPENKADNKIKRGKDGLTLNLIPTQDIAAELGKSKKSNQILVGFALETNNETAHAIDKLKRKNLDFIVLNSLNDEKSCFGHDTNKVCIIDKKGEINNFPLKNKQEVAKDIINKITTWKD",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should take into account the physiological conditions of the Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway.2. The protein requires Mg(2+) to activate a critical catalytic site.3. The designed protein must be optimized for phosphopantothenoylcysteine decarboxylase activity, metal ion binding.4. The designed protein should contain one or more DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains that are essential for its function.5. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.. The designed protein sequence is ",
    "response#1": "MNIVLGITAGIAAYKTPQLVRLLTKNGHTVKVILTENAKEFVTPLTLSTVSKNTVFSAFTSVENDWHSHVELALWADVMLIAPATANTIAKMAHGICDNLLLATYFSAKAPVFVAPAMDLDMYAHPTVTENLKQLQSFGNHIIPAAYGELASGLIGQGRMAEPEDIVTFLEAELIGKLPLAGKKLLVTAGPTQEAIDPVRCLTNHSSGKMGYAIAKMAMLRGAEVTLVSGPTAIEPPLFVKVVPVTSARDMFEAVTGLSDEQDIIIKAAAVADYRPKQVSEDKVKKKDDQASIELERTDDILKYLGQHKKQGQFLCGFSMETRDMLRNSRAKLEKKNLDMVAANNLKVEGAGFQGDTNVLTLITAAGQEDWPRMSKAQLAEKLAEKIAQQFA",
    "response#2": "MNIVLGITAGIAAYKTPQLVRLLTKNGHTVKVILTENAKEFVTPLTLSTVSKNTVFSAFTSVENDWHSHVELALWADVMLIAPATANTIAKMAHGICDNLLLATYFSAKAPVFVAPAMDLDMYAHPTVTENLKQLQSFGNHIIPAAYGELASGLIGQGRMAEPEDIVTFLEEQLAGGRDLAGKKVVITAGGTVERIDPVRYISNDSSGKMGFALARAARAMGAAVTLIAARTDEAPPRDAGIDLVRVQSAQEMHDAVMDRWRDCDILVKAAAVADYRPRESSDSKIKKSGSTMTLELVKTTDILESLGRIKDKQFLIGFAAETGNAEAYAKDKLVRKNLDLIVANDVAVEGAGFGTDTNIVKLFDDAGHHELGKADKLTLAHVLLDRVKDLL",
    "response#3": "MNIVLGITAGIAAYKTPQLVRLLTKNGHTVKVILTENAKEFVTPLTLSTVSKNTVFSAFTSVENDWHSHVELALWADVMLIAPATANTIAKMAHGICDNLLLATYFSAKAPVFVAPAMDLDMYAHPTVTENLKQLQSFGNHIIPAAYGELASGLIGQGRMAEPEDIVTFLEEAINSSDLDLKGKTILITAGPTIEPIDPMRYITNYSTGKMGYSIAQVAADRGANVILISGPTNLTPPRLVKIVNIKTANEMYNQAHKYFDKADIIIKTAAVADYRPEIEHKEKIKKSDNNLTLKLVSNKDILKSLGKIKNEKVLVGFAAETENLIDYAKEKLVKKNLDFIVANDISEQGAGFGVDTNIISIIDKNGKIEKLPKMNKDELSKKIIDEIADIL"
  },
  {
    "reference": "MDLMGMMGKLKETQQKIEDTKKRLDTVLIDEQSADGLLKITVTANRKVKSISIDDSLLEDKEQLEDYLVVTLNKVIEKATSVNERELDAVARMDMPSIPGMDNLFK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that localizes to cytoplasm and can perform DNA binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "LTVLSLPAAFSSTELDAGSQLLLSSFTNQDRLTGNVLDLGCGAGVIGAYLKQRFPAISLTMSDIHSMAIHSAQATLAKNKLTGTVIASDVFSHINDRFDLIVSNPPFHDDIDTAYHTVESLIMQAKNHLNYGGELRIVANAFLPYPDLLDKAFGSHQVVTKSNKFKVL",
    "response#2": "LTVLSLPAAFSSTELDAGSQLLLSSFTNQDRLTGNVLDLGCGAGVIGAYLKQRFPAISLTMSDIHSMAIHSAQATLAKNKLTGTVIASDVFSHINDRFDLIVSNPPFHDDIDTAYHTVESLIMQAKNHLNYGGELRIVANAFLPYPDLLDKAFGSHQVVTKSNKFKVL",
    "response#3": "LTVLSLPAAFSSTELDAGSQLLLSSFTNQDRLTGNVLDLGCGAGVIGAYLKQRFPAISLTMSDIHSMAIHSAQATLAKNKLTGTVIASDVFSHINDRFDLIVSNPPFHDDIDTAYHTVESLIMQAKNHLNYGGELRIVANAFLPYPDLLDKAFGSHQVVTKSNKFKVL"
  },
  {
    "reference": "MAPKAKIVLVGSGMIGGVMATLIVQKNLGDVVMFDIVKNMPHGKALDTSHTNVMAYSNCKVSGSNTYDDLKDADVVIVTAGFTKAPGKSDKEWNRDDLLPLNNKIMIEIGGHIKNNCPNAFIIVVTNPVDVMVQLLHQHSGVPKNKIVGLGGVLDTSRLKYYISQKLNVCPRDVNAHIVGAHGNKMVLLKRYITVGGIPLQEFINNKKITDQELDAIFDRTINTALEIVNLHASPYVAPAAAIIEMAESYIRDLRKVLICSTLLEGQYGHKDIFAGTPLVIGGNGVEQVIELQLNADEKKKFDEAVAETSRMKALI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The NAD(+), substrate binding site should be located in a region of the protein that is accessible to the ligand.2. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for (S)-lactate + NAD(+) = H(+) + NADH + pyruvate.3. A protein with L-lactate dehydrogenase activity, oxidoreductase activity, NAD binding is needed to enhance pyruvate biosynthetic process, glycolytic process in a specific context.4. A protein that can modulate the overall flux of metabolites through the (S)-lactate from pyruvate: step 1/1, Fermentation pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MKVGIVGSGFVGSATAYALVLQGVAREVVLVDLDRKLAQAHAEDILHATPFAHPVWVRSGWYEDLEGARVVIVAAGVAQRPGETRLQLLDRNAQVFADVVPKILKAAPEAVLLIATNPVDVMTQVAYRLSGLPPERVVEAGLAAGDVTILDTARFKYELAEEFGVSDRSVHGQIIGEHGDSELAVWSQANIAGQPLYQLLIDDPEKQHRIEEIFVNTRDAAYDIIQAKGATYYGIAMGLVHITKAILNNQNVVLTVSSRLEGEYGQEDVYIGVPTKINRQGAVEVFEIPLNDEEKTLFTRSVGILKEMQNKISELLA",
    "response#2": "LLTCPMNPYLEQELDKRFKLFRFWDFPSANDLFREHSNSIRAVVGNSFIGADAQMIEALPKMEIVSSFSVGLDKIDLVRCKEKGIRVTNTPDVLTEDVADLALALILATLRRICESDRYVRSGSWKKGDFKLTTKFTGKSVGIIGLGRIGSAIAKRAEGFSCPISYHSRTEKPGTNYKYYPSVVELASNCQILVVACALTPETRHIINREVINALGPKGVVINIGRGLHVDEPELVSALVEGRLGGAGLDVFENEPNVPEELLAMDNVVLLPHVGSGTVETRKDMADLVLGNLEAHFLNKPLLTPR",
    "response#3": "LLTCPMNPYLEQELDKRFKLFRFWDFPSANDLFREHSNSIRAVVGNSFIGADAQMIEALPKMEIVSSFSVGLDKIDLVRCKEKGIRVTNTPDVLTEDVADLALALILATLRRICESDRYVRSGSWKKGDFKLTTKFTGKSVGIIGLGRIGSAIAKRAEGFSCPISYHSRTEKPGTNYKYYPSVVELASNCQILVVACALTPETRHIINREVINALGPKGVVINIGRGLHVDEPELVSALVEGRLGGAGLDVFENEPNVPEELLAMDNVVLLPHVGSGTVETRKDMADLVLGNLEAHFLNKPLLTPEP"
  },
  {
    "reference": "MNITNINTTRIPAGTRLYLTKKKYNKFYIQPDKTLVNDNLFVAYDVKIGGVIAIPQGTRVLGNWVAESSPSIAVQLQLTKIFLYGSGQNISADSDLVETLVDYNSDEIDCAPYLYKIHHFKSPAGIYRRIVNTKCRSKILTDNNRNSIYLEVNTKEISVVLNEDIIPMPDLSKMPVPQSPSVPTMPSVNNDQPTQKPNKITRNYKPKHQHNYKPNYQPNYQPNYGQNCSNSHSNDYFSDYPTEHSGDSSSYSTYEDDDF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should exhibit Polar residues compositional bias.2. The designed protein localizes to the virion component. The designed protein sequence is ",
    "response#1": "LRPVVIDGSNVAMSHGNKEVFSCRGILLAVNWFLERGHTDITVFVPSWRKEQPRPDVPITDQHILRELEKKKILVFTPSRRVGGKRVVCYDDRFIVKLAFESDGVVVSNDTYRDLQGERQEWKRFIEERLLMYSFVNDKFMPPDDPLGRHGPSLC",
    "response#2": "LRPVVIDGSNVAMSHGNKEVFSCRGILLAVNWFLERGHTDITVFVPSWRKEQPRPDVPITDQHILRELEKKKILVFTPSRRVGGKRVVCYDDRFIVKLAFESDGVVVSNDTYRDLQGERQEWKRFIEERLLMYSFVNDKFMPPDDPLGRHGPSLF",
    "response#3": "LRPVVIDGSNVAMSHGNKEVFSCRGILLAVNWFLERGHTDITVFVPSWRKEQPRPDVPITDQHILRELEKKKILVFTPSRRVGGKRVVCYDDRFIVKLAFESDGVVVSNDTYRDLQGERQEWKRFIEERLLMYSFVNDKFMPPDDPLGRHGPSLL"
  },
  {
    "reference": "MGTDNHSVRVSVRGIYATALTKRLLDADYEIVQASPVIRERFDIDFDTVYNTVTVTTTDDHQGVGIHGTESAVRSVADVLTSCGRDTLAWDDLTPPGVIFTAQITETQGSGAICALIPAENPDTESKTEPDLNDVHIPDESVATGYLPYDSTDKHIEQGDIMTVQSHRSMPPWDNTDQRADAVVGTTLRANGGLATLIQGHEGVTVDTYDDEDARELAGMIELIDIDLPTGWGIEVNHAATQASLTALKSGLERAIDRAEDISDGAVAEEVTSTGRWLWFGRESRFALDEIRRSVITTIHGHHRIKAAGETASAGVDLAEALCGDIGDGNVQIQHNEFPFEVVTQQFGPTEGERVELYHGKPEGHAFSLGQGEVTDWDANGTIEVTRRISGHGSGTYDELGTPRESGDTAVTRVREGRWWYPTVYRSRDGEHKGTYVNICTPIECFPKSIRYVDLHVDVVKYPDGTVERVDDDELNTAVTAGHIPPALAEKAQSVASSLERALDK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Binds to RNA in loop regions with AU-rich sequences.; Probable RNase involved in rRNA stability through maturation and/or degradation of precursor rRNAs.2. The designed protein should be optimized for endoribonuclease activity, producing 5'-phosphomonoesters, mRNA 3'-UTR AU-rich region binding activity in order to improve rRNA processing. The designed protein sequence is ",
    "response#1": "ILKPLFDDKDINDKRVKCNDVSGDSSVPVLHCNEVIEKLSALQDNDITSFDDIFFKELKIKEEHYTEKDRYISDPGFLNKVLDLIFGKFSGNDYPKTLTFLLTHPLFPLSRTRNLLSLLRDQPRLFKQAIVTCPNLPLNELLEELFSIRNRELLLDISFRILQDFTRDSIKQEMKKLSKLDVQNFIEFITSGGEDSSPECFNPSQSTQLFQLLSLVLDSIGLFSLEGALLENLTLYIDKQVEIAERNTELWNLIDTKGFQHGFASSTFDNGTSQKRALPTYTMEYLDC",
    "response#2": "ILKPLFDDKDINDKRVKCNDVSGDSSVPVLHCNEVIEKLSALQDNDITSFDDIFFKELKIKEEHYTEKDRYISDPGFLNKVLDLIFGKFSGNDYPKTLTFLLTHPLFPLSRTRNLLSLLRDQPRLFKQAIVTCPNLPLNELLEELFSIRNRELLLDISFRILQDFTRDSIKQEMKKLSKLDVQNFIEFITSGGEDSSPECFNPSQSTQLFQLLSLVLDSIGLFSLEGALLENLTLYIDKQVEIAERNTELWNLIDTKGFQHGFASSTFDNGTSQKRALPTYTMEYLDC",
    "response#3": "ILKPLFDDKDINDKRVKCNDVSGDSSVPVLHCNEVIEKLSALQDNDITSFDDIFFKELKIKEEHYTEKDRYISDPGFLNKVLDLIFGKFSGNDYPKTLTFLLTHPLFPLSRTRNLLSLLRDQPRLFKQAIVTCPNLPLNELLEELFSIRNRELLLDISFRILQDFTRDSIKQEMKKLSKLDVQNFIEFITSGGEDSSPECFNPSQSTQLFQLLSLVLDSIGLFSLEGALLENLTLYIDKQVEIAERNTELWNLIDTKGFQHGFASSTFDNGTSQKRALPTYTMEYLDS"
  },
  {
    "reference": "MARIRVLDADTVSRIAAGEVVERPAAVVKELVENALDAGARVVHIDIAAGGLERITVTDDGCGMEPGDAELALQRHATSKITAAEDLEAVRTLGFRGEALPSIAAVSRLTLRTRPAASSAGTVLETEGGLVLSAGVSGGPPGTVVTVRDLFFNTPARKKFVRSAAHEGAMISEMVGRLALSRPEVAFRLTVNGRQVLATSGSGDLLDAIGAVFGAAVAREMVPVRFEERGLGVSGYVGRPGVSRSSRRHQVFFVNGRYIRSAYLGAAAEESLHGAMPAGRHAVLVLHLSVEPGMVDVNVHPAKHEVRFSQPRDVYVVVHRAVREALHREIQIPVEECGHLESACFPGAGTGFRIPVQVTFQGGEPELREESTGYQGFPQLQPLGFLPPAYILAGGADGLYILDQHAAHERVLFEQYLRTLEQSAGKQLLVPVMVEIRGREAQTLEDYGPFLRQAGFEVDPFGEGAYLIRTVPSFLRPGAEAVLLTDVLDRLAGERPVDADAFRRVVAAVLACHRAVRGGDKPAGPEAAALLTDLGRCAEPYLCPHGRPTLIRIGFPELARRFQRE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that This protein is involved in the repair of mismatches in DNA2. The protein must exhibit ATP binding, ATP hydrolysis activity, ATP-dependent DNA damage sensor activity. The designed protein sequence is ",
    "response#1": "RRMYIVTGPNMSGKSTYIRQVGLIAVMAQIGSFVPADDAEIPIFDRVFTRMGARDDISTGKSTFLIEMSEVALILEKATKKSLVLLDEVGRGTSTFDGISIAWAMSEYIYNEIGCETMFATHFTELTELSDVYEGIKNLTIEVRETNNGVVFLHKVVEGIADRSYGIEVAQIAGVPDGVS",
    "response#2": "GYDPDLDALRRAHADPVAYFLDLEVREKESTGIPTLKVGYNAVFGYYLEVTRPYYEKVPQEYRPVQTLKDRQRYTLPEMKERERELYRLA",
    "response#3": "EDNHKIPTNSGIDNLLDGGVEKGTVTQIFGPPGSGKSNISLVLAVNVAKQGKKVVYVDTEGGISINRIKQIAGEDFPKIVNNIIVFEPTSFLEQNENLKTIELWIRKHHDDVDLCVLDSAVALYRVDDMKSSRLNKELGKQMGILAKIARNYDVAVVLTNQL"
  },
  {
    "reference": "MSFDCDKKSPSSTCAPVIVALDYEDQKSALDFAQLIDPSSCRLKIGKEMFTRFGPQFVTQLQQQGFDIFLDLKFHDIPNTVARAVAAAADLGVWMVNVHASGGSRMMRAAKESLRSFGKEAPLLTAVTVLTSMDQSDLTELGINLTPAEQAERLALLAKACGLDGVVCSAHEATRFKQVCGDDFLLVTPGIRPAGSEVGDQRRVMTPEQAIVAGVDYMVIGRPITRSENPLETLQQINRSIQGVVQHG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a high specificity for substrate binding, with minimal off-target effects.2. The protein must exhibit the following characteristics: Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP)..3. The designed protein should interact with enzymes and substrates within the UMP from orotate: step 2/2, Pyrimidine metabolism, UMP biosynthesis via de novo pathway pathway to enhance or regulate its activity.4. The designed protein should have orotidine-5'-phosphate decarboxylase activity to facilitate 'de novo' pyrimidine nucleobase biosynthetic process, 'de novo' UMP biosynthetic process.5. The Proton donor of the protein must be engineered to enable effective catalysis of H(+) + orotidine 5'-phosphate = CO2 + UMP. The designed protein sequence is ",
    "response#1": "MKLCIALDNPSKEENLKLAKELKEYAKELWLKVGFRSYIRDGAEFIKELKLMGYNVFLDLKLYDIPNTMADAAEEIANLGVDMFNVHASAGSIALKTVMQRLEKYEKRPLVLAVTALTSFTDEEFKKIYGDSIENKAKEFAKLSHEAGLDGVVCSAFESKMIKNITDEKFITLTPGIRPFGEDAGDQARVADISLAKEQKVDFIVVGRPIYKDEDPKAKVEKIQEKINEYK",
    "response#2": "MKLCIALDNPSKEENLKLAKELKEYAKELWLKVGFRSYIRDGAEFIKELKLMGYNVFLDLKLYDIPNTMADAAEEIANLGVDMFNVHASAGSIALKTVMQRLEKYEKRPLVLAVTALTSFTDEEFKKIYGDSIENKAKEFAKLSHEAGLDGVVCSAFESKMIKNITDEKFITLTPGIRPFGEDAGDQARVADISLAKEQKVDFIVVGRPIYKDEDPKAKVEKLISEIK",
    "response#3": "MILALDVTEREEALKIAENVREFVDAIKVGYPLILATGLDIIRELARFAPVIADFKVADIPNTNRLICEQVFKAGADAVIVQGFTGRDSLDACIEVASKYGKDVFVVSEMSHPGGAEFLQSAAEAIAKMAVEAGAFGLVAPATRPERVKEIRKIIGDRLTIISPGVGAQGGKASDVISAGADWVIVGRSIYKAESPKEAACPVYVAIDTTEAARAIALAERLKGQVGGFKLGLEYFTANGPAGMEAVSGLGMPLFVDLKLHDIPNTVAAAMKGVVRLGAAITTIHASGGAAMIRAAVDAANDEAAKLGIAPPAVVAVTVLTSLDQAGAEQVGFERPVLDQVKRLATLAQDSGAAGIVCSPLEVEAVRALCGPDFKLVIPGIRPAWSEAGDQKRFLTPAEARAKGADVLVIGRPITAAADPAEAAGRLRAASL"
  },
  {
    "reference": "MSWKFVERPKPGTETALDACDYFVDEVIESSRGRDAREEVRQIKHGELRAAVSTGDERTVRVLLEALGRERQIIVNMAPAGANTLLFIASQSGFESITQRLIDAGADGRSHAVTKYSPLYAAVHNGHYGIAKLMLERFPELIQQATVERWLPLHAACINGHIKMLELIIGFPYPDYLYQTYRDEEGQWEWRLPFDANAQDVTGQTSLYIASLLGNKQLLGVLLKWQLRCRRTLSEDSGSSTTGQSTPITPTRKRISFGIQAIMSKLHISGEGDALEAQPTQEQESQRCPIHINLLCGAARETALLAAVRGGHLDVAQALLQHGANPNIVAKPVEDHNDPKCSEEIYGLSNMPITEACKQRSLPMLDLLLKFGARDDNGTAISVAIASEDEALLSRLLARRVHPDSDYKINKKGLPTPVEVNVFLPSASNISYSAMFPNVPTIIDWHSLSMNVQLPFVRIGWLVSGVLQLNPKLSSHPRLNEVALTAITRIDFSHNLLTSIPPELFNLVSLKYLNVAQNKITELPAPLDKTYNCPVLDELFLQDNQLTTLPAAIFHLPALTILDISNNKLQQLPFDMWRAPKLRELNVAFNLLRELPVPPMQTSGSLMSLDKLQLLQSCDEPSSNKPHTLLHRTLTHRNLWSTALDITDNDMKWQQDQEQEQEQAVAEGKTPYGASQLSSLNIANNLFTSIPAALPCLAVNLTRLNMSYNSLRSMGHVTSYPATLKQLDLSHNEISCWPSLPRITESDPHLLCYSHVQLPEGMDEPIYK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have ATP binding within cell body to regulate locomotion, retrograde transport, endosome to Golgi, synapse organization, lysosome localization.2. The ATP binding site should be located in a region of the protein that is accessible to the ligand.3. The protein should have a fast turnover rate for ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-   threonyl-[protein].4. The designed protein have Roc domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "KLTVRLQYVQEGKQIETVIQILEDMMLLTFTDHAAKLFKGKNVHIPLLLVLDSYMRVASVQQAGWSLLCKLTEICPDIMQSLMGPQDVGKDWEVLGVHQLILKMFTVHNANITLSVIGLKTLDQLLTSDLMTLRILEEDCSDVFTLIFDAMHTFTTNEEIQKFGCKVLHVLFGKVSEEQLTEFIENKDYMFLLNALKRFNHEEEVVFHVLHSLHSLALPCSNVEVLMSGNVRCYNIVVEAMKMFSNSEKIQEVGCCLLHRLTLDNFFNILVLNEVHEVIVKAVKKYPMNAPLQIAALSCLALLTETIILNRDLEEKNENQEDDEDNKLCWLEASYRALTLHRKNTHVQEAACWALNNLLIYQSNLHENIGDEDDQYPAHREMVLSMLMHSSSKEVFQAAANALSTLSEQNVNIRKALLTKGIYWNVLQLMMRHLDFPEVAESGCRMLNHLLEGSNLNLDVMASVTSHIIEVMKNHKTSSAVQLEAFRAILHIIVPESKKEHEEDSSLNINLKVIKNSCLLNDIHKLVLGALNKFIGNPGIQICGLKIISSLVNCSGALEMFSQQGALDSVLHTLQMYPDDRGIQCLGLSLLGSLITKKNLCTETQDLLANILVSSLQRFKDVVEVQISGV",
    "response#2": "EVLLGKGRFAEVWRARLLQGEKGGVNSFETVAVKVFPAVEYASWRNECSIFSDSKLEHENVVRFLAAEERGALGHTLREYWLVLDYHGHGNLQDFLTTNILSWEELVAMAGGVAKGLAHLHSDTMPSGVPKVPVAHRDLKSSNIVVKSRTDCALCDFGLALRLDLSLTADDYANSGQVGTARYMAPEVLESRVNLEDLEAFKQMDVYSMALVLWEMASRCHAIGEVKSYEPAFGSKVCEQPCVDSMRDLVLRDRGRPDIPSAWTQHQGMNVFCFTITECWDHDPEARLTAHCVSK",
    "response#3": "KLTVRLQYVQEGKQIETVIQILEDMMLLTFTDHAAKLFKGKNVHIPLLLVLDSYMRVASVQQAGWSLLCKLTEICPDIMQSLMGPQDVGKDWEVLGVHQLILKMFTVHNANITLSVIGLKTLDQLLTSDLMTLRILEEDCSDVFTLIFDAMHTFTTNEEIQKFGCKVLHVLFGKVSEEQLTEFIENKDYMFLLNALKRFNHEEEVVFHVLHSLHSLALPCSNVEVLMSGNVRCYNIVVEAMKMFSNSEKIQEVGCCLLHRLTLDNFFNILVLNEVHEVIVKAVKKYPMNAPLQIAALSCLALLTETIILNRDLEEKNENQEDDEDNKLCWLEASYRALTLHRKNTHVQEAACWALNNLLIYQSNLHENIGDEDDQYPAHREMVLSMLMHSSSKEVFQAAANALSTLSEQNVNIRKALLTKGIYWNVLQLMMRHLDFPEVAESGCRMLNHLLEGSNLNLDVMASVTSHIIEVMKNHKTSSAVQLEAFRAILHIIVPESKKEHEEDSSLNINLKVIKNSCLLNDIHKLVLGALNKFIGNPGIQICGLKIISSLVNCSGALEMFSQQGALDSVLHTLQMYPDDRGIQCLGLSLLGSLITKKNLCTETQDLLANILVSSLQRFKDVVEVQISGI"
  },
  {
    "reference": "MKKMAIVLAAGQGKRMKSKLYKVLHAVCGKPMVSHVLDAVRGASCERSIVIVGHGAEAVQSTLGVGGSIEYALQAEQLGTGHAVMQAKPMLEDEEGVTIVICGDTPLVRSETVEAMIALHLQQGASATILTAELNDPAGYGRIVRGADNGVLRIVEHKDCSVEEAAIKEINTGTYCFDNRKLFQALNQVTNTNAQGEYYLTDVIGILQSEGNAIAAYLVDDPAEAIGVNDRIALGEAERFMRLRINRGHQVNGVTLIDPQNTYIESEVKIGADTIIYPGTILRGQTSIGSDCIIGPASELQDSSVGDGTTIRQSVTEGAEIGEGCNIGPYAYLRPGTKLGQNVKIGDFVEIKNTVIGDGSKVPHLSYVGDAVVGSNVNIGCGAITANYDGYNKSKTEIGDNAFVGSNSNLIAPVKIGSGAYIVAGSTITHNVSDNDLAIARQRQVNKPGYAEKIRARARIKKEEKQSD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have at least one Nucleotidyl transferase domain.2. Ideal protein should have a binding affinity for key enzymes in the Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway to enhance the overall efficiency of the pathway.3. The designed protein should have magnesium ion binding, glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity within cytoplasm to regulate UDP-N-acetylglucosamine biosynthetic process, peptidoglycan biosynthetic process, cell morphogenesis, regulation of cell shape, lipid A biosynthetic process. The designed protein sequence is ",
    "response#1": "ALILAAGKGTRMKSDLPKVLFEAAGKPMIDYVVDAAKGVSASEINVVIGNGAELVRDHLGGEVNFSLQETQRGTGDAVMAAAAEVERFDGKVLILCGDMPLIQSETLNKFIEESADKDVSFISVKVKKPEGYGRVVRGLDDSVLKIVEEKDASDEEKRINEVNTGIYLCDSGVLLERLKNIDSNNAQGEYYLTDIVAEDSSEILGVNDRVQLASAGAMLNRKTNRLLMDRWMLEGVSIIDPDHTYISAEAIIGKDTVIYPGTMIQGDSVIGEECIIGPNSEIKDSKIGNQTTIRQSVAHDSEIGDSVAIGPFAHIRPSSTISDDVKIGNFVEIKKSVMGKGSKASHLSYIGDAEVGADVNLGCGSITVNYDGKNKFLTKIEDGAFIGCNSNLIAPVTIGKGAYVAAGSTVTNDVPEKALSVARSRQRNIDGWVERKRGNKK",
    "response#2": "AVILAAGAGTRMKSKLPKVLHDVCGKPMVEHIIDAAEEVNAKKTIVVIGHKGDKVKKAIEHRGVDFALQKEQLGTGHAVIKAEELIEDEGNVLLLCGDTPLIKGETLKALIDFHIKGNFQGTILTADFENPKGYGRIVRDDEGNVVKIVEEKDANEQEKRITEINSGMYCYNAKLLKEALKKITNDNKQQEYYITDVIEILRKEGYKVGAFKVEDWVESMGVNDRVALAEAERIMRARINEQHMRNGVTLINPEHTYIGPDVHIESDVTIEPGCQITGETTIKTGSHIGSNSEITDSIIGENSLVKQSVVTNSQVGREVNIGPFAHIRPESEVGDQVKVGNFVEVKKSQLGTNSKVSHLSYIGDADVGKDANIGCGTITVNYDGKNKYKTIIEDEAFIGCNSNLVAPVTVKKGAYVAAGSTITKDVPEKALSIARSRQVNKEGYVKKMGVTLIDPKTTYIGIDVEIGKDTIIYPNNILEGNTKIGSNCVLYQNSRIADSVVGNEVDIQSSVILNSNIGDNTTVGPFAYIRPETTIGKHARIGDFVEIKKSTIGDGTKVSHLTYIGDAEVGSECNFGCGTVVVNYDGKEKHKTIIGNHSFIGCNTNLVSPVKVEDNTYIAAGSTITSEVKEGELAIARAKKEIKKDW",
    "response#3": "TIILAAGKGTRMKSSQIKVLHPVAGKTIINHVITALDGLQSKIVNVIGYQKNEVQTELDKIAGVDLNYVIQEEQLGTGHAVKQAAEYIKNHQGPVLILYGDTPLLKQKTLAKFVAEHSSNSRDLTILTAKLADPTGYGRVITDQKGSLSRVIEEKDADQKIKQIKEINSGVYCVDSKLLISFLNNMDNNNAQHEYYLTDLIAYAAKKNCQTGAFIASNVSELIGINNRVQLAKAEAIMRERINEMHMLNGVSIIDPDHTYISAEAIIGKDTVIYPGTMIQGDSVIGEECIIGPNSEIKDSKIGNQTTIRQSVAHDSEIGDSVAIGPFAHIRPSSTISDDVKIGNFVEIKKSVMGKGSKASHLSYIGDAEVGADVNLGCGSITVNYDGKNKFLTKIEDGAFIGCNSNLIAPVTIGKGAYVAAGSTVTNDVPEKALSVARSRQVNKEGWVSKK"
  },
  {
    "reference": "MTICQFFLQGRCRFGDRCWNEHPGARGAGGARQPPPQQQPPSGNNRRGWNASSQRYSNVIQPSSFPKSTPWGGSRDQDKPPFGSFDSGASTSRGFGSSQNPFASPLSDEQKDEKKLLEGIVKDVEVWESSGQWMFSVYSPVRKKPNISGFTDISPEELRLEYHNFLTSNNLQSYLNSVQQLVSQWRNRINELKNLTMSTKGALLSDVKDGVSQAVPAFGFGSKQAGSFGSPGFPVNNSSSSTVQNFSFKTSPGLATPPSGSTSVFGSHPAFGAGPSAGSSISSSTPAFGLGKPEATSAASFSFKSPEASSFASPGFSGFPASMAASPSGSTTAPPLRSGSSVVGFGSPSPHSQAVFAKPSTDVFGGSGISTSVLASGAADNALFTPRDQLMKEELEQFQSQRFTLGKIPLKPPPVELLTV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that can perform metal ion binding in nuclear pore, nucleoplasm, cytosol to support nucleocytoplasmic transport.2. The protein should exhibit Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "WAPFHVVRHHNIPDKVFEHLNAGEVFTKLGLFAEIGYAWASIDSSLFLWDYTHPNPELIGYEEATHTITAVALVPPKPGVFVKTITHVLVVATTSEIILLGVSATPTPSGSKSLTLYSTRMSVHRGGSDVSFIVGTKDGRIFLGGESDTDIHEIFYQQEERWFSSRCGKINHSHPGWSAVVPSLAGLPFGSRQQEWLRGLYVDDTRNLLYSLSNRSTIRTYHMEGPEKLTKVIEKDKTSCLRDFAHMADSSPLFTDKTNIVALSPIPATEASKLHLMALTDTGCRLFLSATSSASYTMGGATSLAPQSMQLQFVKFPPRESPTRIRTLNGQIIDSQLDKTSRALDPSALGFRFSPGYFFDVVRKHPNQDMLFVSAPDTGRIKVTQPASALKYFEQGTWIELENGNRTIEIGLTTAPFAAAKQPLGFGNELAVQFDQVPGEFAVLTNTGVHR",
    "response#2": "WAPFHVVRHHNIPDKVFEHLNAGEVFTKLGLFAEIGYAWASIDSSLFLWDYTHPNPELIGYEEATHTITAVALVPPKPGVFVKTITHVLVVATTSEIILLGVSATPTPSGSKSLTLYSTRMSVHRGGSDVSFIVGTKDGRIFLGGESDTDIHEIFYQQEERWFSSRCGKINHSHPGWSAVVPSLAGLPFGSRQQEWLRGLYVDDTRNLLYSLSNRSTIRTYHMEGPEKLTKVIEKDKTSCLRDFAHMADSSPLFTDKTNIVALSPIPATEASKLHLMALTDTGCRLFLSATSSASYTMGGATSLAPQSMQLQFVKFPPRESPTRIRTLNGQIIDSQLDKTSRALDPSALGFRFSPGYFFDVVRKHPNQDMLFVSAPDTGRIKVTQPASALKYFEQGTWIELENGNRTIEIGLTTAPFAAAKQPLGFGNELAVQFDQVPGEFAVLTNTGVHR",
    "response#3": "WAPFHVVRHHNIPDKVFEHLNAGEVFTKLGLFAEIGYAWASIDSSLFLWDYTHPNPELIGYEEATHTITAVALVPPKPGVFVKTITHVLVVATTSEIILLGVSATPTPSGSKSLTLYSTRMSVHRGGSDVSFIVGTKDGRIFLGGESDTDIHEIFYQQEERWFSSRCGKINHSHPGWSAVVPSLAGLPFGSRQQEWLRGLYVDDTRNLLYSLSNRSTIRTYHMEGPEKLTKVIEKDKTSCLRDFAHMADSSPLFTDKTNIVALSPIPATEASKLHLMALTDTGCRLFLSATSSASYTMGGATSLAPQSMQLQFVKFPPRESPTRIRTLNGQIIDSQLDKTSRALDPSALGFRFSPGYFFDVVRKHPNQDMLFVSAPDTGRIKVTQPASALKYFEQGTWIELENGNRTIEIGLTTAPFAAAKQPLGFGNELAVQFDQVPGEFAVLTNTGVHR"
  },
  {
    "reference": "MNKAELITSMAEKSKLTKKDAELALKALIESVEEALEKGEKVQLVGFGTFETRERAAREGRNPRTKEVINIPATTVPVFKAGKEFKDKVNK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be able to perform DNA binding, structural constituent of chromatin under specific conditions.2. The protein must exhibit the following characteristics: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.. The designed protein sequence is ",
    "response#1": "PKRSLSAYMFFANENRDIVRAENPGISFGQVGKLLGEKWKALTPEDKIPYENKADTDKKRYEKEKAEYL",
    "response#2": "MLSKKAKAPKPSGDKKRRRKRKESYGIYIYKVLKQVHPDTGISSRAMSIMNSFVNDVFERIAAEASRLAHYNKKSTITSREVQTAVRK",
    "response#3": "MNNPKRSLSAYMFFANENRDIVRAENPGISFGQVGKLLGEKWKALTPEDKIPYENKADTDKKRYEKEKAEA"
  },
  {
    "reference": "MERSDLPPDVVIRRLPLYARSLRYLLEEGVHSVSSQELGERINVTAAQIRKDLSYFGEFGKQGIGYDVEKLLQHIERILGLHHHWPVALVGIGLLGQAIARYEGFRTEGIEIVALFDSDPAKIGQKIGDLTIQDFAHVRRIIAEKQIKMAIIAVPAQQAQRVADVLVEAGIRAILSYAPMILQVPEDVWVRYIDPVAVLQSMTYYLAREQEH",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in response to redox state. The designed protein sequence is ",
    "response#1": "HPQLSFVGRVEATDSVDLIARVDGFLDKRTFTEGQAVKTGDLLFVLQKDALQAALDAAQANLAKAQADADNLKLQTERARSLYKQKTVSQAMLDDRVAAEKQALAVVQQAQASLEQAQINLGYTDIRAPFSGRIGMANFSVGALVGPSSGPLATIVSQDPIYVTFPVSDKTILDLTEGGRTATDRSNVAVSLTLSNGMTYPQTGAIDFTGIKINPNTDTLMVRAQFPNPNNVLIDGQYLS",
    "response#2": "HPQLSFVGRVEATDSVDLIARVDGFLDKRTFTEGQAVKTGDLLFVLQKDALQAALDAAQANLAKAQADADNLKLQTERARSLYKQKTVSQAMLDDRVAAEKQALAVVQQAQASLEQAQINLGYTDIRAPFSGRIGMANFSVGALVGPSSGPLATIVSQDPIYVTFPVSDKTILDLTEGGRTATDRSNVAVSLTLSNGMTYPQTGAIDFTGIKINPNTDTLMVRAQFPNPNNVLIDGQYL",
    "response#3": "HPQLSFVGRVEATDSVDLIARVDGFLDKRTFTEGQAVKTGDLLFVLQKDALQAALDAAQANLAKAQADADNLKLQTERARSLYKQKTVSQAMLDDRVAAEKQALAVVQQAQASLEQAQINLGYTDIRAPFSGRIGMANFSVGALVGPSSGPLATIVSQDPIYVTFPVSDKTILDLTEGGRTATDRSNVAVSLTLSNGMTYPQTGAIDFTGIKINPNTDTLMVRAQFPNPNNVLIDGQYL"
  },
  {
    "reference": "MREDTGDFRRLFLSGTPMMDVRAPLEFARGAFPGTVNLPLMDDEERHQVGLCYAEKGQHAAIELGHRLVWGALKATRIAQWADFARAHPDGYLYCFRGGLRSQIVQQWLRDEAGIDYPRVTGGYKAMRGFLIDVIEQAAAKQDFLVLGGLTGTGKTDVIAEVPAAVDLEGLACHRGSAFGRRAQPQPQQIDFENSLAIDVLQRAEAGYRALVVEDEGRFIGGRDLPKVLWQRMQASPMVWLEASFEERVERVLRDYVLGLAAEHIEQLGPVAGFEAYATRLRDAMAAISPRLGSERYGRLSALLEQALTSQSERGETAPHRAWIEVLLRDYYDPMYAYQEEKRAARVVFRGDRAAVTDWLRERSAR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should contain one or more Rhodanese domains that are essential for its function.2. The designed protein must have a S-selanylcysteine intermediate that is structurally stable and resistant to denaturation.3. The designed protein should have transferase activity, transferring alkyl or aryl (other than methyl) groups to facilitate tRNA wobble uridine modification. The designed protein sequence is ",
    "response#1": "DTRAPIEFTKGAFPGVLNLPLMTDQERQRVGTCYKQQGQQAAIVLGHQLVSGAIKEQRIQAWADFARAHPDGLLYCFRGGLRSQIVQQWLREAGIDYPRVGGGYKAMRTFLLDTTC",
    "response#2": "MGGQGEVLSLMSGGFDSTVASYLTMKRGLKTHFIFFNLGGAAHEIGVKQVALYLWNKFGASHRVKFVSVPFDGVVDEIFRASHESYMGVMLKRLMIKAAEQVAHNMGIDALVTGESVAQVSSQTLRNLAIIDEASNKLVLRPLAVMDKPEIMAIADKIGTREFAESMPEYCGVISRNPVVGGH",
    "response#3": "MGGQGEVLSLMSGGFDSTVASYLTMKRGLKTHFIFFNLGGAAHEIGVKQVALYLWNKFGASHRVKFVSVPFDGVVDEIFRASHESYMGVMLKRLMIKAAEQVAHNMGIDALVTGESVAQVSSQTLRNLAIIDEASNKLVLRPLAVMDKPEIMAIADKIGTREFAESMPEYCGVISRNPVLA"
  },
  {
    "reference": "MGITLPSHDRLPRAPCTPTSSARTMPETPHPRTILRGHKAQVHALAFVGNNDRLASGDAEGYVALWDLTIMRPTAVWQPHDNAILGIQGWGADRIITHGRDHKLAVWKLATSDEANLSKKLPLDDTSEPRPQPWLLHLIDVNTMNFCAFAACVPTDPPPAGDDPELLLAVPNTLASESIDIYHLPTQTRRHTVKSPGQNGMVMALALVPQGDSLTLLAGYENGVTTAMHLSAASGTWNTTYRTQPHSQPVLSLDVSPDGLSFVTSSADAVVARHPVPPPAGMEVLEQPLKVVNTKHSGQQSLRFRDDGRVFATGGWDAMVRVYSGKTMKEVAVLKWHEVGCYAVAFASALKAPQAAGGVEERDGTVVRRAGEVSVRERRIAHAEAAHWLAAGSKDGKISLWDIF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Component of the ASTRA complex involved in chromatin remodeling.2. The protein should be optimized for stability and solubility when expressed in nucleus where it will be involved in chromatin organization. The designed protein sequence is ",
    "response#1": "MVGQLAARRAAGVVLEMIREGKIAGRAVLIAGQPGTGKTAIAMGMAQALGPDTPFTAIAGSEIFSLEMSKTEALTQAFRRSIGVRIKEETEIIEGEVVEIQIDRPATGTGSKVGKLTLKTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSPHGIPIDLLDRLLIVSTSPYSR",
    "response#2": "MVGQLAARRAAGVVLEMIREGKIAGRAVLIAGQPGTGKTAIAMGMAQALGPDTPFTAIAGSEIFSLEMSKTEALTQAFRRSIGVRIKEETEIIEGEVVEIQIDRPATGTGSKVGKLTLKTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSPHGIPIDLLDRLLIVSTSPYSR",
    "response#3": "MVGQLAARRAAGVVLEMIREGKIAGRAVLIAGQPGTGKTAIAMGMAQALGPDTPFTAIAGSEIFSLEMSKTEALTQAFRRSIGVRIKEETEIIEGEVVEIQIDRPATGTGSKVGKLTLKTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITRIRGTSYQSPHGIPIDLLDRLLIVSTSPYSR"
  },
  {
    "reference": "MNIYRTHLCDQLRKEHINQEVTLSGWIYRKRDHGKIIFVDLRDHYGITQLVFNEADNQNFQLITHLRLESVITVKGIVVARDSSTINTAVSTGFIEVVVKHVIIEGEADPLPLNITSTQDYPEEIRLKYRFLDLRRDKVKNNIILRSKIISELRKSMEAMGFIEIQTPILTSSSPEGARDYLVPSRIHHGKFYALPQAPQLFKQILMVSGFDKYFQIAPCFRDEDARSDRSPGEFYQLDIEMSFVSQEDVFNVIEPVLLNVFSKFSNKTIDKEFPRISYHDAMLHYGSDKPDLRNPLIIQDVTEIFRDSQFNIFNSNIKQGMVVRAIPAPNTATNPRSFFDNKIEFAKTLGAQGLGYITFNDDFSAKGPIAKFLDEERLNRIKSICNLQPGDSVFFVSETEDKATELAGEVRTLLGKELNLIEKDTFRFCWIIDFPYFKYDKKEKSINFFHNPFSMPQGGLEALNNQNPLDILAYQYDIVCNGIEISSGAIRNHKLDIMYKAFSIAGYTKEMVDQKFNSLTRAFKFGAPPHGGIAPGIDRMVMLLADATNIREVICFPLNQSGEDLLMSAPSEIDKEHLKLLSLSITKKS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The ideal protein would be one that plays a critical role in aspartyl-tRNA aminoacylation through aspartate-tRNA(Asn) ligase activity within cytoplasm.2. The protein should be able to bind L-aspartate ligand in a variety of conditions3. The protein must exhibit the following characteristics: Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn); Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).. The designed protein sequence is ",
    "response#1": "MFASAVQNGGQVKAINVKGAADKYSRKDIDALTEYVARYGAKGLAWLKVEADGLKGPIAKFFTEDEQKQMMQTLEAETGDLLLFVADKKSVVADAE",
    "response#2": "FKMRSRAAIAARNSLDKMGFIEFETPVLTRATPEGARDYLVPSRVYPGQFYALPQSPQLFKQLLMCSGFDKYFQIAKCFRDEDLRADRQPEFTQIDIEMSFVEQEDIINMAETMLKDIFKACGHDIKTPFRRMSYKEATETYGSDKPDLRYDLKMIDVIDIFERSSNEIFSSIAKDKKKNRIKALKVPNGDNIFSKREMNRFEEFVRKFGAQGLGYFQMKEDGLKGPLCKFFEQSDLDEIVSRCELKVGDVVFFGAGKKKIVLDYMGRFRIFLAEQMGIIDQDRLEFLWVLDFPMFEQNDDGSYSAMHHPFTMPKNIDEPDLEDILSIAHDVVLNGFELGGGSIRIHKNDIQQKVFKLLGIDEAEQREKFGFLLDALTFGAPPHGGIAIGFDRLNMLVNKASSIRDVIAFPKTASASCLLTEAPSPAADPRGRVAALHVPGGAGLTRKQIDDYTQFVGRYGAKGLAYIKVNDPAQGREGLQSPILKFLTDEAVDGILRRTEARAGDLIFFGADKAKVVNDAP",
    "response#3": "FKMRSRAAIAARNSLDKMGFIEFETPVLTRATPEGARDYLVPSRVYPGQFYALPQSPQLFKQLLMCSGFDKYFQIAKCFRDEDLRADRQPEFTQIDIEMSFVEQEDIINMAETMLKDIFKACGHDIKTPFRRMSYKEATETYGSDKPDLRYDLKMIDVIDIFERSSNEIFSSIAKDKKKNRIKALKVPNGDNIFSKREMNRFEEFVRKFGAQGLGYFQMKEDGLKGPLCKFFEQSDLDEIVSRCELKVGDVVFFGAGKKKIVLDYMGRFRIFLAEQMGIIDQDRLEFLWVLDFPMFEQNDDGSYSAMHHPFTMPKNIDEPDLEDILSIAHDVVLNGFELGGGSIRIHKNDIQQKVFKLLGIDEAEQREKFGFLLDALTFGAPPHGGIAIGFDRLNMLVNKASSIRDVIAFPKTQFRAIAEEGGTVRAINAKACAHFSRKDLDGLTEYAALFGAKGLAWVKMKADGEWQSPIAKFFSEEERASIAEALDAQEGDLLFFGADSEKVVFQK"
  },
  {
    "reference": "MKTLCTHSLPKEKMPYLLRSGEGERYLFGRQVATVMANGRSTGDLFEIVLLSGGKGDAFPLHVHKDTHEGILVLDGKLELTLDGERYLLISGDYANIPAGTPHSYRMQSHRTRLVSYTMKGNVAHLYSVIGNPYDHAEHPPYASEEVSNERFAEAAAVADIVFLDEAKPACSAKLAELTELPDGAVPYVLESGEGDRLLTGDQLHRIVAAQKNTDGQFIVVSSEGPKGDRIVDHYHEYHTETFYCLEGQMTMWTDGQEIQLNPGDFLHVPANTVHSYRLDSHYTKMVGVLVPGLFEPFFRTLGDPYEGHIFPCEPQALRFDRILQNIEALDLKVMKP",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that is capable of catalyzing reactions within the Flavonoid metabolism pathway with high efficiency and specificity.2. The designed protein must be able to perform metal ion binding, quercetin 2,3-dioxygenase activity under specific conditions.3. The desired protein should be able to perform O2 + quercetin = 2-(3,4-dihydroxybenzoyloxy)-4,6-   dihydroxybenzoate + CO The designed protein sequence is ",
    "response#1": "CVDMDQKVIVNYYPKCPQPNLTLGLKRHTDPGTITLLLQDQVGGLQATRDGGKTWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNSSRMSIATFQNC",
    "response#2": "DQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDGGESWITVKPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSSTSRLSIATFQNC",
    "response#3": "LASGDEQDSEALHIRADAEVAAVTLKAGQSAEYALENGRRAYLVPATGSIEVNGVRAEARDGLAVRDEPTLKVTALEDSEVLLVEC"
  },
  {
    "reference": "MKVLLTNDDGFHANGIKVLKEIVMAAGIASEIWVVAPLSNCSGCGRSVGLRHAIEVYKVSDTEFIVNSTPSTTMFLGLKEIVGEKPDLVLSGINSGVNIGNDVTYSGTIAAAAEAAMMSIPSIAISQEYDGRSGEINWENPRKFLKGIVDMLLGAPSWDKSTVMSVNFPLISAKGIKFTSQGKYMPYNKIEKKKNAASSISYTIHRTAPDKDSRGESDDSIRALDDGYVTITPLKFDMTDFDILESLMLLNEGCNI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein localizes to the cytoplasm. The designed protein sequence is ",
    "response#1": "CCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGK",
    "response#2": "CCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCK",
    "response#3": "RRRRRGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCV"
  },
  {
    "reference": "MEDRLNIRDMSLPEIEALITTLGKEKYRARQIMKCLYQKGATSFAEMTALSREFRARMEDLVVIAEPVLDRIQTSADGTKKALFGLADGLFIESVLIPGRNHWTACISTQAGCAMGCLFCLTGRRGLMRNLSPSEITGQMTMLRRFTPEGPEIKNIVLMGMGEPLANYDHTVTAIRILTSDYGLGFSNRKITVSTCGLAPQIVRLGRDICVNLAISLNAPDDRRRNALMPVNRKYPIATLLEACRDYPMPGRRMLTFEYILMAGVNDSPADARKLALLLKNIRCKLNLITFNEFSASPFRTPSPEAVSAFQQVLLDHRYTAILRASRGRDILAACGQLSGTRDAE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be involved in rRNA base methylation by localizing to cytoplasm and exhibiting tRNA (adenine-C2-)-methyltransferase activity, metal ion binding, 4 iron, 4 sulfur cluster binding, rRNA (adenine-C2-)-methyltransferase activity, tRNA binding.2. The designed protein is the optimization of the S-methylcysteine intermediate, Proton acceptor to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.3. The designed protein must have at least one Radical SAM core domain.4. The protein must exhibit the following characteristics: m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.5. The protein should have a high affinity for S-adenosyl-L-methionine, [4Fe-4S] cluster. The designed protein sequence is ",
    "response#1": "DGNRGTLCISSQVGCQLNCTFCSTARQGFNRNLSTAEIIGQVWTARRLLDQPDTRPQAVTNVVFMGMGEPLLNLSAVTDAIDIMLDDHGYGLSRRRVTVSTAGVIPAFAALRERVPVSLAVSLHAPTDELRNQLVPLNRKYPIAQLLAACKTYVAEESRQKVTFEYAMLRGINDQPSHARTLAKLLQKVPAKINLIPFNPFPDAPYQRSDDETILRFQQILITAGLITTIRRTRGDEIDAAVGQLSGRVVSDTPTNLYDLTRPALGALLSDWGFRPHHRDVLWTGLYRQQVRALDEVEGLRSDLRDTLRERTRLSHLATHHEAFSSDGHTHKLLLRLEDGQTIETVLMRFKGRATVCISTQAGCAMGCVFCATGQMGLSRHLTPGEIVGQILHVQRILRETGESLRNVVLMGMGEPLHNYDNTLAAVDVLVDPLGLAMGPRFITLSTVGVVPGIRRLADEDRPVQLAVSLHGATDAERAALVPAGRRWPLAELMDACRYYIARRKRRIFFEWTLIAGRNDTSEHAHTLGALLGGMDAHVNVIPLNPTVGYDGDPSQPASVRAFQDVLMSHGVPSTVRQRRGIDIDAGCGQLKSAVERRSRG",
    "response#2": "DERIVTQCISSQVGCAMGCVFCASGVAGLKRQMTDAEIVAQVLLGRRELRPDQRIRNVVFMGMGEPLHNYDAVARSLVLLTHPDGIDLSKRRVTVSTSGLVKQIDRLGKDFDGQVQLAVSLHAVRDEDRSKIMPVNRKHGISELTDALRRYPMSKRARITIEYTLIKDVNDSVRDANGMVELLRGIPVKVNLIPMNPISASDLQAPDSSHVLRFQEQLTRRGLSVFVRTTQGDDVGAAASLASDTPTNLYDLTRPALGALLSDWGFRPHHRDVLWTGLYRQQVRALDEVEGLRSDLRDTLRERTRLSHLATHHEAFSSDGHTHKLLLRLEDGQTIETVLMRFKGRATVCISTQAGCAMGCVFCATGQMGLSRHLTPGEIVGQILHVQRILRETGESLRNVVLMGMGEPLHNYDNTLAAVDVLVDPLGLAMGPRFITLSTVGVVPGIRRLADEDRPVQLAVSLHGATDAERAALVPAGRRWPLAELMDACRYYIARRKRRIFFEWTLIAGRNDTSEHAHTLGALLGGMDAHVNVIPLNPTVGYDGDPSQPASVRAFQDVLMSHGVPSTVRQRRGIDIDAGCGQLKSAVERRSRLA",
    "response#3": "EEDRGSVCLSSQVGCTLTCRFCHTGTQTLVRNLGAGEIVGQFMAARDAYGEWPSPKGEAPRLLSNIVLMGMGEPLYNYENVAQAMRIAMDGEGIALSRRRITLSTSGVVPMMDRCGAELGVNLAVSLHAVRDELRDELVPLNRKYPLTMLLEACRRYPGASNARRITFEYVMLKGVNDSEADARELVRLIHGIPAKVNLIPFNPWPGSRFETSPASAIRRFAEIVMAAGYASPISAAPTRQNRSDTPTNLYDLTRPALGALLSDWGFRPHHRDVLWTGLYRQQVRALDEVEGLRSDLRDTLRERTRLSHLATHHEAFSSDGHTHKLLLRLEDGQTIETVLMRFKGRATVCISTQAGCAMGCVFCATGQMGLSRHLTPGEIVGQILHVQRILRETGESLRNVVLMGMGEPLHNYDNTLAAVDVLVDPLGLAMGPRFITLSTVGVVPGIRRLADEDRPVQLAVSLHGATDAERAALVPAGRRWPLAELMDACRYYIARRKRRIFFEWTLIAGRNDTSEHAHTLGALLGGMDAHVNVIPLNPTVGYDGDPSQPASVRAFQDVLMSHGVPSTVRQRRGIDIDAGCGQLKSAVERRSRS"
  },
  {
    "reference": "MTFATMLQALEGCVPVLEHHGDLNIAVKGITDDSRAVSAGSVFVAVKGERVDGHQFIPMVVNAGVGGVVAQQPMETGTVPFVYVEDSRKALGLLGSQFYGDPSSRLRMVGVTGTNGKTTTTYVCKAFLEAVRARVGMIGTVAYQIGDTVIPAAHTTPGALELQQLLAKMVTHGCTTAVMEVSSHALAQDRTSGCEYDVAVFSNLTQDHLDFHGTMEAYFQEKLRLFTGLANGQKSNKRVIVNVDDSYGPRIVEQCSVPVWTYALKTKADLRAEDVKLSLQGVSFTAVTPRGNFRVESHLVGEHNVYNLLAAIGVALHEGATPDQIHHAVTQVMNVPGRFERVMAGQQFTVVVDYAHTEDALNRLLMAAETVKTGRIITVFGCGGDRDRGKRPKMGEAAVRSSDVVILTSDNPRTEDPHVILEQVEQGVVRALRQRPHVQYHKIPDRREAIETAVREAREADMVLIAGKGHEDYQIIGTTKYHFDDREVARQAIERLKFQT",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have a high affinity for meso-2,6-diaminoheptanedioate, ATP.2. The protein design should be able to enhance the efficiency of cell wall organization.3. The protein should be optimized for activity in Cell wall biogenesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. Incorporating a Meso-diaminopimelate recognition motif motif is critical for the successful design of the protein.5. The designed protein should contain one or more Mur ligase N-terminal catalytic, Mur ligase C-terminal domains that are essential for its function. The designed protein sequence is ",
    "response#1": "ITGTNGKTTTAAILRSILEAGGGRAGTLGTTGILWTGPGGEVAREATHTTPEGPEFYRWLRQMVDDEVEAVAVELSSHALHQGRVAGLAIDVAAWSNLGRDHLDYHHDLANYEAAKALLLSEWLATWGKSDATFVLNVDDPIVRRHVTDWSRVLRVSSRPGAVVRGDADLAPLEPPAMSLDGIAVPVQWESEEGLLTTSLLGEHNLANCLLAGGLAIAAGEEPAAVFAALETLTGASGRMQKAATRENGATVFVDYAHTPDALVTALQAMRPHVMGRLIVVFGAGGDRDTGKRPLMGAAARDHADVVFVTDDNPRSEDPAAIRAAVMEAVPDATEVGDRAEAILRGVDALGPGDTLLIAGKGHETGQIVGDDVLPFDDAEQASLAVAALGG",
    "response#2": "VTGTSGKTTTTYLLERGLTAAGHKVGLIGTTGTRIAGRDVPSKLTTPEAPALQALFARMRDEGVTHVVMEVSSHALMLGRVGGTDFDVAGFTNLSQDHLDFHPTMEDYFEAKALFFDPASPIAATKSVVCVDDEWGERMAQRAAHPVTVGTHGQEGLDVQAHQVSVEESGAQHIALTHDGHDYEFRLPMPGAFNVANAALAVGMATLLGVEDSEILRVLRELKPAPGRLERVDAGQPFTVVVDYAHKPDAVEAALGTLRPLTESKLIAVIGAGGDRDSGKRPIMGEISARLADHVIVTDDNPRTEDPASIRRQVLEGTTGARAIVEEIGDRRAAIGRAIELAEPGDIVVVAGKGHETCQIVGDRVLPFDDREVARAALEAR",
    "response#3": "VTGTNGKTTTTHLIQAVLEGAGRPTGLLGTIGNRLGDKVLPTAHTTPEALDLQALLHQLVNLGAQGVVMEVSSHALALHRVEGAEFDVAVFTNLTQDHLDFHRDMEDYFKVKARLFQALGKGVKGGPKYAVINGDDPYSARLITLTPVPVVTYGCSPECQVRARDINLTAGGATCRVTWPGGEVELNLKLTGRFNIYNALAAFTVG"
  },
  {
    "reference": "MTQSVAAILVAAGRGERAGGLTDGPKQYRRIGGRAVLAHALDIFRSHPRVGPIVVVIHADDAVLCRDALGDAADDVQIVTGGAERQISVRCGLEALAASGTLPEIVLIHDAVRPFTDPATIDRVIAAAASHGGAIPAVAIADTIKRAAAGGAIAETVPRSGLYGAQTPQGFRFAAIRDAHAAAAAGSGRTDFTDDASIAEWAGLAVMLVEGAAGNVKLTTREDILTADARLKETPPMIDVRTGNGYDVHRLVAGDHVTLCGVRIPHDRRLDGHSDADVGLHALTDALLATCGAGDIGDHFPPSDPQWKGAASHIFVEKAVEIISAKGGRIMNADVSLICEAPKIAPHRRAMREAIAAMTGLTLDRVSVKATTNEAIGFVGRKEGIAAIATASVVYGETA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D- erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C- methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).2. The designed protein should contain one or more 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domains that are essential for its function.3. The protein should be designed to have high stability and activity towards 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-   erythritol 2,4-cyclic diphosphate + CMP.4. The designed protein should have 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity, metal ion binding to facilitate terpenoid biosynthetic process. The designed protein sequence is ",
    "response#1": "IRLGNGYDVHRFGEGDHVWLCGVKVPHGRSLQGHSDADVGMHALTDAIYGALARGDIGQHFPPTDPQWKGAESHIFLRHAIGLAREMGYEMMNCDVTLVCERPKVGPHAASMRAALADIMGVDVEKVSVKATTSERLGFTGREEGIAALATAAEVVSARTGTGYDVHALGEGDGIWLCGVHIDHDKRLVGHSDADVALHALTDALLATLGDGDIGTHFPPSDPQWKGAASKIFVAHAVEKVRARDGRIANADITLICEAPRVGPHRAEMTQALHEMLGISAGRISIKATTNEKLGFIGRSEGIAAFATVSVL",
    "response#2": "MAVRLGNGYDVHRFGEGDHVWLCGVKVPHGRSLQGHSDADVGMHALTDAIYGALARGDIGQHFPPTDPQWKGAESHIFLRHAIGLAREMGYEMMNCDVTLVCERPKVGPHAASMRAALADIMGVDVEKVSVKATTSERLGFTGREEGIAALATAALIASHFFGDPSSELRLVGVLGTNGKTTTCYLVEAILRAGGRSAGLITTVRYRIGDREVEASRTTPEAPDLQRLLGEMRDAGVEYAAMEVSSHALDQGRVTGCLFDAAVFTNLTQDHLDYHCDMEEYFAAKARLFTRLGNGYDVHRFGDGDHVILCGIAVPHDRGLQGHSDADVGMHAVTDAIYGALAEGDIGRHFPPSDPQWKGAASEIFLRHAVDLSVSKGYAIGNVDCTLICEYPKIGPHAAAMQQEMARIMGLAPDQVSIKATTSERLGFTGRGEGIAALATATLVRTGQGFDVHQFEPGDAVILCGVAIPHAGKLKGHSDADAAMHALTDAVLGAISEGDIGDHFPPTDPKWRGAPSRIFLEKAMALVRDRGGDVVHCDITIICEAPKIKPHRTAMRAALAEIMGVSVERVSVKATTTEELGFTGRREGLAAMATATFSLPGQFDVHNALAAVGAALHEGVPLEAIAGALAAEPGVPAVLVHDAAGASDADVALHAITDALLHDLEGDA",
    "response#3": "RLGNGYDVHRFGDGDHVILCGIAVPHDRGLQGHSDADVGMHAVTDAIYGALAEGDIGRHFPPSDPQWKGAASEIFLRHAVDLSVSKGYAIGNVDCTLICEYPKIGPHAAAMQQEMARIMGLAPDQVSIKATTSERLGFTGRGEGIAALATATLVLGTDPAPLADAAARARVGTGYDVHAFGPGDHVWLGGVRIGHDQGVVAHSDGDVALHALTDALLGALADGDIGTHFPPSDPQWRGAASAQFLAFAAARVRERGGRIDFLDLTVVCETPKIGPHREAIRAEIAAIAGISLGKVAIKATTSERMGFTGRGEGLVALATATGGAVLG"
  },
  {
    "reference": "MILKNVYDVFMERGFIEQVTDENAVRKALEAPLACYIGFDPTARSLHIGSLVPIMALIHLQRHGHTSIALVGGGTALIGDPSGKTEMRQILTREKIELNATCMRRQFARYLSFEDKKAMMINNADWLTKLNYISFLRDIGRHFSVNKMLAAESYKMRLEKGLNFIEFNYMLLQAYDFLYLFQNHNCVMQMGGNDQWGNMLAGVDLIRRVEGKVAHSMTFPLLTTATGQKMGKTEKGAVWLDRELTSPYEYYQYWINTGDIDVGKFLALFTFLPMEEIHQVKSLSDKELNMAKAILAFEATKITHGEDAALAAWNASAVAFGVKLIDTSLMPSSTIPRGQLSQDASAIPFIKKSWNELAKGIPAYEIMHECGLCSSKSEARRLIAQGGGYVNENPIFAFDELITTEHLDRSGQIKLRKGKKKYMIIKVE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein's functional design necessitates the inclusion of a 'HIGH' region motif. The designed protein sequence is ",
    "response#1": "XGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCR",
    "response#2": "APRAMVVLHPLKVVITNLEAKSAIEVDAKKWPDAQADDASAFYKIPFSNVVYIERSDFRMQDSKDYYGLAPGKSVILRYAFPIKCTEVILADDNETILEIRAEYC",
    "response#3": "APRAMVVLHPLKVVITNLEAKSAIEVDAKKWPDAQADDASAFYKIPFSNVVYIERSDFRMQDSKDYYGLAPGKSVILRYAFPIKCTEVILADDNETILEIRAEYR"
  },
  {
    "reference": "MDVEKLIAESKKAREQAYVPYSKFPVGAALLAEDGTIYHGCNIENSAYSMTNCAERTAFFKAVSDGVRSFKALAVVADTEGPVSPCGACRQVIAEFCNGSMPVYLTNLKGDIEETTVAKLLPGAFSKEDLSYAAEQ",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must have a Proton donor that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "SEWFFLDFINPSSANLIKARRLIFSARSPYQEIEILDTQAFGKCLMLDGRMQSAQADEFIYHEALVHPAMVTHPNPESVLIVGGGEGATLREVLRHRTVRRAVMVDIDRQVVEACRLFLPEWSDGAFEDPRTELVIDDARHYLTSTDEQFDVIIMDITEPLENGPSAPLVTYEMFQIVRSHLKPQGIVAHQSGSTGTSELHFFASMYKTLRAVFRYVAPYSIQITSFAMPWGIVIASL",
    "response#2": "SEWFFLDFINPSSANLIKARRLIFSARSPYQEIEILDTQAFGKCLMLDGRMQSAQADEFIYHEALVHPAMVTHPNPESVLIVGGGEGATLREVLRHRTVRRAVMVDIDRQVVEACRLFLPEWSDGAFEDPRTELVIDDARHYLTSTDEQFDVIIMDITEPLENGPSAPLVTYEMFQIVRSHLKPQGIVAHQSGSTGTSELHFFASMYKTLRAVFRYVAPYSIQITSFAMPWGIVIASV",
    "response#3": "SEWFFLDFINPSSANLIKARRLIFSARSPYQEIEILDTQAFGKCLMLDGRMQSAQADEFIYHEALVHPAMVTHPNPESVLIVGGGEGATLREVLRHRTVRRAVMVDIDRQVVEACRLFLPEWSDGAFEDPRTELVIDDARHYLTSTDEQFDVIIMDITEPLENGPSAPLVTYEMFQIVRSHLKPQGIVAHQSGSTGTSELHFFASMYKTLRAVFRYVAPYSIQITSFAMPWGIVIASC"
  },
  {
    "reference": "MRPPPFPKPAHPEEALALQRALAKKVRLAGSLEGVRRIAALDASHKRGRPLVAVALLYDLEKGPLHVATALLPEEALFPYVPGLLSFREAPAYLEALAALPEAPEALLVDGQGVAHPRGLGIASHLGVHLDLPSVGVAKRLLYGRPEAPLPEEKGAAVRLLAPDGRPLGYVYRSRTGVKPLYVSPGHRVGLEEALRFVRRLPTRFRLPEPLRLAHLEAGRALKALD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a binding site that is specific to the target Mg(2+) ligand.2. The protein should have a low Km value for Endonucleolytic cleavage at apurinic or apyrimidinic sites to   products with a 5'-phosphate..3. For general function, the protein need meet that Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA.; DNA repair enzyme involved in the repair of deaminated bases4. A protein with deoxyribonuclease V activity, magnesium ion binding functions is required to enable DNA repair. The designed protein sequence is ",
    "response#1": "MGIRGLAKLIEEVAPAAVSKRLIQHYCNKVIAIDASVMLYQFITTITSGDGTALANSSGEVTSHLVGLLAKVIRLAEAGIKPIFVFDGKPPEDKQGELEKRRQAREAG",
    "response#2": "NKVIAIDASVMLYQFITTITSGDGTALANSSGEVTSHLVGLLAKVIRLAEAGIKPIFVFDGKPPEDKQGELEKRRQAREAAELEQQKAEEEGNLERAKQLSRRTVKVTQQHCKQAERLLDTLGIPYVVAAGEAEAQCVAMAKERVCEGVASSDLDVLAFGSPCLIRNLAQGGDREIVEINLNTVLKELGFSYDEFLDLCILCGCDYANSLEGIGPKTAYKLIVKHRSIEEVLAADQGKLAEKALNWPYVRARELFKNPAVIDPKEIKKTLKWKPVNRGEAMQFLVEEMEFDRETTEKKLGIPYIEAYGEGEAQAAYLVENGDAWAVASQDYDCLLFGAKRVVRNLAINSNLGDLEYYNLKRVLDELDINREQLIDMGILIGTF",
    "response#3": "MGVTELGKLIGKDIRREVKLENLAGKCIALDAYNSLYQFLASIRQPDGTPLMDRVGRITSHLSGLFYRTINLMEAGIKPVYVFDGKPPEFKLAEIEERRKVKEKRR"
  },
  {
    "reference": "MHFSSIFRMYNVVFVLGPPGAGKGTLCSMIQEKLGYKHLSAGELLRQERIRKESAFGEIIENHIKNGTIVPVEITCKLLKNAMAQSGSGKTFLIDGFPRNQDNLDGWEREMKDCANVQLVLNLTCPEEISVERCLGRGQGRSDDNVESLKKRIRTFHEQTLPIIEHYKKLKLVKDVPTVSAPEKVFDETKIFFDEVEHHNIKA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a specific function in 'de novo' pyrimidine nucleobase biosynthetic process, phosphorylation and should be localized to cytoplasm in order to carry out this function efficiently.2. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.3. The protein should have a high affinity for ATP. The designed protein sequence is ",
    "response#1": "MNTRLLLGTNNPGKVRELRAIL",
    "response#2": "MTITP",
    "response#3": "GFPVL"
  },
  {
    "reference": "MSLHNKNILLGISGGIAAYKCAELTRRLKDHGANVRVVMTKGGKAFITPLTMQAVSGNPVSDSLLDPAAEAGMGHIELAKWADLVLVAPASANTLARLSAGMADDLLGTLCLATPAPIALAPAMNQQMYLAPATQENLTRLAQRGVHIWGPAAGEQACGDVGPGRMLEPNDLVERCLQHFQAGSALAGLKLLITAGPTREALDPVRYLSNHSSGKMGYAIAEAALAAGAEVTLITGPVNITAPSQAQTIAVCSALEMHHAAMSQAPEHDIFIACAAVADYRPETVADNKIKKNDDGLVVKMIKNPDILADIANSDPRPFCVGFAAETQDVAHYAKGKLNRKNLDLIAANDVAKAGQGFNTDNNALTVFSKTQQFDIAFASKADVAKQLLNIIHQQYNK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that can modulate the overall flux of metabolites through the Cofactor biosynthesis, coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway, enhancing or suppressing its activity as needed.2. The Proton donor of the protein must be engineered to enable effective catalysis of H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.3. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.4. The designed protein should have phosphopantothenoylcysteine decarboxylase activity, FMN binding, metal ion binding to facilitate coenzyme A biosynthetic process.5. The protein contains novel Flavoprotein domains that confer a unique function or activity.6. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.7. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.. The designed protein sequence is ",
    "response#1": "IGLGVSGSIAAYKAVALLRLLRAEGAEVAVMLTPAATRFVGSLSFAALSRHAVETDVMELLPDGRIGHIVLADSVDAIVVAPATAHWLAAMAHGLSDDVLTATCLASRAPVVVAPAMDGEMWSHPATAANVARLRDDFGYRIVEPTSGPLASGQDGPGRLAELSDIVEAVVAALAPARPTRPAAPALEGRHIVVSAGGTREAIDPVRFIGNRSSGRMGAAIAAAALDRGARVTVVAANLEVPLPPGATVVTVESTAELRAALLGLTLAPDGRAGFDALIMAAAVADFRPADATPGKIERGSGLTLVLEPTPDVLTQVARLVRGTDSSGATTTRQAEPRPFLVGFAAEIGSFERVADKLRRKGVDLLIANDVAEPGSGFGSETNRVSILFADGAAEPWPLLPKDEVADRVLDRVVPLIR",
    "response#2": "KRILLGITGGIAAYKAAELVRLLVKQGFEVQVAMTEAATQFITPTTMQALSGQPVLLNQWQDEAGMTHIHSSRAADAIVIAPATADFIAKLAHGMADDLLSALCLARDCPLLVAPAMNKQMWSNPATQRNVQQLAADGIAVLGPACGSQACGEEGMGRMLEPAELADEIVAALTGTHRLEGRHIVVSAGGTREAIDPVRFIGNRSSGRMGAAIAAAALDRGARVTVVAANLEVPLPPGATVVTVESTAELRAALLGLTLAPDGRAGFDALIMAAAVADFRPADATPGKIERGSGLTLVLEPTPDVLTQVARLVRGTDSSGATTTRQAEPRPFLVGFAAEIGSFERVADKLRRKGVDLLIANDVAEPGSGFGSETNRVSILFPGADAPELEGRHIVVSAGGTREAIDPVRFIGNRSSGRMGAAIAAAALDRGARVTVVAANLEVPLPPGATVVTVESTAELRAALLGLTLAPDGRAGFDALIMAAAVADFRPADATPGKIERGSGLTLVLEPTPDVLTQVARLVRGTDSSGATTTRQAEPRPFLVGFAAEIGSFERVADKLRRKGVDLLIANDVAEPGSGFGSETNRVSILTPGAAPVEVAAGSKRAVAGAILDAVERLLAD",
    "response#3": "KKILLIITGSIAAYKAMDLVRLLTKKSYDVTCVLTKAACEFITPLLASSLSGNKAHSDLFSVDDEVEMGHIKLSRAADLIVIAPASADFIAKIANGYADDLASSIVLAANKKILIAPAMNEKMWENKQTQTNLVKALESGMSMVEPEKDILACGEFGIGKMASPEKICEEICNELARVLGQRDLGQRILITAGPTREEIDPVRYITNYSSGKMGYALAEMAKKRGALVTLVTGPCQIPIPKVDEAVSVLSTEEMFHAVMSRYEQQDVVIKAAAVSDYRPTDRAVQKIKKQKEGSMELSLTKNPDILWELGKTKNDQVLVGFAAETENLIENATSKMNRKNLDLIVANDLMQPGAGFHCDTNQVMLLTRDGVERLQGRTVLVTAGGTREAIDPVRYVGNRSTGKQGYAVAEAAKARGANVTLISTVAAAAPNGVEVVRVESAAQMHAAVLERSEADVIVMTAAVADFRPVTASDSKIKKVEGPPKLKLEPTPDILAELGALKKPGQVIVGFAAETEDLASHAQSKLERKNVDVIVANDVSAPEVGFAYDTNEVTILDAGGTHEAIPHGSKHDLANVILDAVATLR"
  },
  {
    "reference": "MSSPAKYIFVTGGVTSXLGKGIIAASLAKLLQARGYSVTIQKLDPYINVDPGTLNPYEHGECFVTDDGAETDLDLGHYERFLNVSTSQANNVTTGRIYQYVINQERKGAYLGKTVQVIPHITDEIKRRIKLLDNTGKYDIIITEIGGTVGDIESLPYVEAVRQLKWEPNFGCIVLHLTLVPYLAASGELKTKPTQHSVKALLESGVQPDILALRTEHVLTEGIKEKIALFCNIDPNAVIQAIDADTIYDVPNLMKAEGLDKVVLDKLSLPYGEEPILKAWNEVLYKIKNPKHKISIGLVGKYIDLKDSYKSISEAMIHAGASNDTKVEIKWIHSESITDANVKENLSNLDGILVAPGFGERGIEGKILTIKYAREHKVPFFGICLGMQGAVIEFARNVLGYSKAHSTEMEVNTPNPVIYLMEEQRSVTEKGGTMRLGAYPCNILNNSKVFTAYNETNISERHRHRYEFNNKYKEEFEKAGMIATGINPDKDLVEIVEIEDHPWFVGVQFHPEYKSTVINPHPLFVNFIKASILAKKESVQTANN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in glutamine metabolic process, potentially with improved properties such as stability, specificity or selectivity.2. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.3. The designed protein should contain one or more Glutamine amidotransferase, CTP synthase N-terminal domains that are essential for its function.4. The Nucleophile active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "IQDSYISINEALSHAGASLGARVRRVFIEAEDLEKDSSRVRDLLESADGILLTPGFGSRGVEGMVEAARFAIEGGKPFLGICFGAQLLFVAFMRYVAGLREAHTTEVDPDTPHPVVDLLPEQKRVLEKGGTMRLGAHPVKVKPGTRLYEAYRSELIYERFRHRYHINPEYVPLAESKGLVVSSTDPSGRIVNSIELRGPAWIVGVQFHPEYKSRPGKPSPIYREFL",
    "response#2": "LPDAYLSVTEALRAGGFAHRARVEIRWVQSDLCETPAGAAAALEGVDGVLVPGGFGIRGIEGKLGAVTHARTRGIPTLGLCLGLQCMVIETARNVAGLEGASSTEFDPATAHPVISTMAEQVDVVAGERDMGGTMRLGAYPATLVDGGIVAKAYGTTEVSERHRHRFEVNNAYRDRLAEAGLRFGTSPDGTLVEFVELPAEVHPFFVGTQAHPELKSRPTRPHPLFAAFVR",
    "response#3": "LPDAYLSVTEALRAGGFANHAKVEIKWVPSDDVATEEGAAATLSDVHGVLVPGGFGVRGIEGKIGAVRFARQRNIPVLGLCLGLQCMVIDVARNLAGLTGAHSAEFEADSRDPVISTMVEQKDVVAGERDMGGTMRLGAYPARLVEGTQVAKAYGRTDISERHRHRYEVNNDYRDRLGKAGLLFSGLSPEGKLVEFVELPSDVHPFYVGTQAHPELKSRPTRPHPLFDAFIA"
  },
  {
    "reference": "MLGKYVCETEPLSPGLRRLMWRFLQNKNLNTFHAQELRFIHLVLCKMYNFGLNVYLLREATANAGTYDEVVLGRKVPAEVWKLVYDGLEEMGVSSEMLLCEAYRDSLWMHLNDKVGLLRGLANYLFHRLGVTHDVRIAPENLVDGNFLFNLGSVLPCRLLLAAGYCLAFWGSDEHERWVRFFAQKLFICYLIVSGRLMPQRSLLVWASETGYPGPVEAVCRDIRSMYGIRTYAVSGYLPAPSEAQLAYLGAFNNNAV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Plays a role in the expression of late transcripts bridging viral DNA replication and late gene expression during infection..2. The designed protein localizes to the host cell cytoplasm, host cell nucleus. The designed protein sequence is ",
    "response#1": "EHGFIHAAGTIQANCPQLFMRRQHPGLFPFVSAIASSLGWYYQTATGPGADARAAARRQQAFQTRAAAECHAKSGVPVVAGFYRTINATLKGGEGLQPTMFNGELGAIKHQALDTVRYDYGHYLIMLGPFQPWSGLTAPPCPYAESSWAQAAVQTALELFSALYPAPCISGYARPPGPSAVIEHLGSLVPKGGLLLFLSHLPDDVKDGLGEMGPARATGPGMQQFVSSYFLNPACSNVFITVRQRGEKINGRTVLQALGRACDMAGCQHYVLGSTVPLGGQ",
    "response#2": "EHGFIHAAGTIQANCPQLFMRRQHPGLFPFVSAIASSLGWYYQTATGPGADARAAARRQQAFQTRAAAECHAKSGVPVVAGFYRTINATLKGGEGLQPTMFNGELGAIKHQALDTVRYDYGHYLIMLGPFQPWSGLTAPPCPYAESSWAQAAVQTALELFSALYPAPCISGYARPPGPSAVIEHLGSLVPKGGLLLFLSHLPDDVKDGLGEMGPARATGPGMQQFVSSYFLNPACSNVFITVRQRGEKINGRTVLQALGRACDMAGCQHYVLGSTVPLGGR",
    "response#3": "EHGFIHAAGTIQANCPQLFMRRQHPGLFPFVSAIASSLGWYYQTATGPGADARAAARRQQAFQTRAAAECHAKSGVPVVAGFYRTINATLKGGEGLQPTMFNGELGAIKHQALDTVRYDYGHYLIMLGPFQPWSGLTAPPCPYAESSWAQAAVQTALELFSALYPAPCISGYARPPGPSAVIEHLGSLVPKGGLLLFLSHLPDDVKDGLGEMGPARATGPGMQQFVSSYFLNPACSNVFITVRQRGEKINGRTVLQALGRACDMAGCQHYVLGSTVPLGGC"
  },
  {
    "reference": "MKVSAALLCLLLTAAAFSTQVLAQPDAINSPVTCCYTFTGKKISSQRLGSYKRVTSSKCPKEAVIFKTILAKEICADPEQKWVQDAVKQLDKKAQTPKP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that can perform CCR chemokine receptor binding, chemokine activity in extracellular space to support neutrophil chemotaxis, positive regulation of ERK1 and ERK2 cascade, positive regulation of synaptic transmission, glutamatergic, G protein-coupled receptor signaling pathway, cellular response to tumor necrosis factor.2. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "DNILVVFILVKYKGLRQAENMSFLNLALSNLGFLLTLPFWAYAASHGEGFDDPLCKILLLLYSIGLYSEAFFNVLLTVQRYKEFFHVRRRFSACRTVAGSIFISVLVWVTATLVTLPELVSYKPQMQSQKYKCFFTGLHFLPADETFWKHFLTLKMNILGFLLPLFAFVYCYVRMRKTLQFRERNYGLFKLVFTIMAVFLLMWGPYNIVLFLSAFNEHFSLHGCGSSYNLNKSVQITRIIAATHCCVNPLLYTLRNKDLKGA",
    "response#2": "DNILVVFILVKYKGLRQAENMSFLNLALSNLGFLLTLPFWAYAASHGEGFDDPLCKILLLLYSIGLYSEAFFNVLLTVQRYKEFFHVRRRFSACRTVAGSIFISVLVWVTATLVTLPELVSYKPQMQSQKYKCFFTGLHFLPADETFWKHFLTLKMNILGFLLPLFAFVYCYVRMRKTLQFRERNYGLFKLVFTIMAVFLLMWGPYNIVLFLSAFNEHFSLHGCGSSYNLNKSVQITRIIAATHCCVNPLLYGLKNRELRK",
    "response#3": "QHLGMTKCNITCHKMTSPIPVTLLIHYQLNQESCGKRAIILETRQHRHFCADPKEKWVQDAMKHLDHQTAALTRNC"
  },
  {
    "reference": "MRVREIMRXYQHLWRWGIMLLGMLMICNGTENLWVTVYYGVPVWKEATTTLFCASDAKAYKAEAHNIWATHACVPTDPNPQEIKLENVTENFNMWKNNMVEQMQEDIISLWDQSLKPCVKLTPLCVTLNCTEWKNSTSTGTNATNENIIEMKNCSFNVTTEVRDRKQQVHALFYKLDVIQMNDNSSSNTSYRLINCNTTTITQACPKVTFEPIPIHYCAPAGYAILKCNDKKFNGTGPCKNVSTVQCTHGIKPTVSTQLLLNGSLAEEEIIIRSENLTDNAKIIIVHLNESVXIXCARPYETATRRTSIGTGQXYXTTIXVKGDIRRAQCNISREDWNKTLQQVAKKLGDLFNMTTIIFKPSSGGDPEITTHSFNCGGEFFYCNTSGLFNSTWGXNDTVNSTXDTGNITLPCRIKQIVNMWQGVGKAMYAPPIAGLINCSSNITGLLLTRDGGVNITTNXSEXFRPGGGDMRDNWRSELYKYKVVRIEPLGLAPTKAKRRVVERQKRAIGLGAVFLGFLGAAGSTMGAASLTLTVQARQXMSGIVQQQNNLLRAIEXQQHLLQLTVWGIKQLQARVXAXKRYLRDQQLLGVWGCSGRHICTTAVPWNSSWSNXSLDEIWQNMTWMEWEREIDNYTGLIYSLIEESQTQQEKNEQDLLELDKWASLWNWFNITQWLWYIKMFIMIVGGLIGLRIVFAVLSVVNRVRQGYSPLSFQTLLPAPREPDRPEGIEEEGGEQGRDRSIRLVNGFSALIWDDLRNLCLFSYHRLR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The ideal protein would be one that plays a critical role in fusion of virus membrane with host plasma membrane, positive regulation of plasma membrane raft polarization, positive regulation of receptor clustering through structural molecule activity within viral envelope, host cell plasma membrane.2. The designed protein have Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains with specific sequence motifs that are crucial for its activity.3. The protein must exhibit the following characteristics: Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Transmembrane protein gp41: Acts as a class I viral fusion protein; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane. The designed protein sequence is ",
    "response#1": "MRVMGTQRNYPPFWRWGMIIFWIMMICNAEKLWVTVYYGVPVWRDAETTLFCASDAKXXEKEVHNVWATHACVPTDPDPQEIXMKNVTENFNMWKNNMVEQMQEDIISLWDQSLKPCVKLTPLCVTLICKELNSTSSNITSXDXTSYNXSMATEMKNCSFNMTTELRDKKKTVYSLFYKLDIVSTEGTNDSYRLINCNTSTMTQACPKVSFEPIPIHYCTPAGFAILKCRDKTFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVMIRSENITDNSKIIIVQLTXPVNITCIRPGNNTRTSIXIGPGQAFYTTGDVIGNIRKAYCHVNGTAWNDTLQKISXXLGXHFXNKTIIFRNSSGGDLEVTTHSFNCGGEFFYCNTTVLFDSIWDKNSTEDKNGTGTNSTXDIILPCKIKQIINMWQRVGQAMYAPPIRGVIRCESNITGLLLTRDGGEEISNVTFRPTGGNMRDNWRSELYKYKVVKIEPIGVAPTRARRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASITLTVQVRQLLSGIVQQQSNLLKAIDAQQHLLKLTVWGIKQLQARVLAVERYLKDQQFLGIWGCSGKLICTTNVPWNNSWSNKSYNEIWDNMTWIEWEREIENYTHTIYNLLEESQNQQEKNEQDLLALDKWASLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSIVRRVRQGYSPLSLQT",
    "response#2": "MRVMGIQRNCQQWWIWGILGFWMLMICNVVGNLWVTVYYGVPVWRDADTTLFCASDAKAYDKEVHNVWATHACVPTDPNPQEISLENVTEEFNMWKNNMVEQMQTDIISLWDQSLKPCVKLTPLCVTLNCNNPNSSSTSFNATNGTISSGEGELKNCSFNITSSIRNKMKKEYALFYTLDIIPIDDDSNTTDSNTTYRLRSCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSKNFSDNAKTIIVQLNESVVINCTRPNNNTRKSITIGPGRAFYTTGQIIGDIRQAHCNLSRTQWNNTLKQIVGKLREQFGNNKTIVFNQPSGGDPEIEMHSFNCGGEFFYCNTTQLFNSTWIFNSTWNDTNTEGNDTITLQCRIKQFINMWQEVGKAMYAPPISGQIRCSSNITGLLLTRDGGINESRTNETFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRAVGIGAVFLGFLGAAGSTMGAASVTLTVQARQVLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTNVPWNSNWSSKSLDDIWDNMTWMQWDKEINNYTQLIYSLLEESQNQQEKNEQDLLALDKWANLWNWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSIVKRVRQGYSPLSFQTLTPNPRGPDRLGGIEEE",
    "response#3": "MRVRETQMNWPNLWKWGTLILGLVIICSASNNKWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLENVTESFNMWKNNMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTHNVTATNS"
  },
  {
    "reference": "MNTEITASEMRRVDANCGGIGLLPLQLMENAGASVACAIRARFEGTQTRIVVLAGTGNNGGDGFVAARHLLDYNPVILLLGRAQDIATEESRRNWAILENLSANLREIRDSSELSELELGADIVIDAMLGTGVRGNIREPVASAIDLINESSAFVLSMDVPSGFNPDNFEKVVHADLTITNHRIKEGLSGHPAVAGEIEVADIGIPTRATLIVGTGDLPPARNAGSHKGDNGRVLVIGGGAFTGAPALAALAAARAGADWVTVAAPKSVAGTIAGFSPDLIVHSLSGSYLTSDDIDTVNTLIRKHDVTVIGMGLGRESETAEAVSGIVRSNPDARFVIDADALHALRMPMPVHEGALPIITPHAGEFRMLGGIVSADLDERCELVMDFSARNHVVTLLKGNIDIISDGRAVRINRTGNPGMTVGGTGDVLAGVTGAIFAKAGALAAASAGALIAGTAGDLAFLKYGYGLLATDVIECIPDAMRG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design must be able to perform NADPHX epimerase activity, metal ion binding, NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding in order to contribute to nicotinamide nucleotide metabolic process.2. The designed protein should be able to compete with endogenous proteins or inhibitors for AMP, K(+) binding.3. The protein should be designed to catalyze the following reaction (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.4. The protein must bind to Mg(2+) in order to perform its enzymatic function.5. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.6. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MREADAFTIESGSMESIELMERASSAFASKIMERWGKDTPVKIFAGSGNNGGDALAVARMLSQSGYKVSVFLFNINGSLSPDCQKNKERLAECPGLEMTEVTSQFVPPTLDRGDLVVDGLFGTGISRPLNGGFASVVKYINSSHSTVVSIDVPSGLMCEDNSYNFMNHVVRADYTFTFQFPKLAFFFPDNEQYVGEWEVLDIGVKDPETPDAVLADVGPQTWRNATDDDVADLLPRPEAESDKYRRGVLGIVAGSDRFTGAAVLAVGGAIRAGAGLVRFASAETAVRQVRNRWPEAITTVLDGPSVAEVGRVQAWVLGPGMGTGEEAVALARAVLATDVPVLVDADGLTILANDRSLLRRSAPTVLTPHAGELSRLLDVPRERIEAARMEHVRRAVAELGAIVLLKGSTTLIAEQDRPVRVNTTGTPWLATGGTGDVLSGIAGTLLSGGLSGYDAVSCAAHLHGLAAQAASTGISGGHGPIAAMDVATMLPQVIRSL",
    "response#2": "MREADAFTIESGSMESIELMERASSAFASKIMERWGKDTPVKIFAGSGNNGGDALAVARMLSQSGYKVSVFLFNINGSLSPDCQKNKERLAECPGLEMTEVTSQFVPPTLDRGDLVVDGLFGTGISRPLNGGFASVVKYINSSHSTVVSIDVPSGLMCEDNSYNFMNHVVRADYTFTFQFPKLAFFFPDNEQYVGEWEVLDIGVKDPETPDSHKYTRGLVTIIAGDMPGASALAASAAMRSGAGVVRLQAREFVPGVPAAVIQMPGDPLDRLDDKRIGALLLGPGLRSGRELLDAALASGHPLVLDAGALAMLADGGPERIEGAILTPHDGEFQKLFGALEGSKVDRARASAAQSGAVIAYKGPDTVVAAPDGRAAIHGTAPPWLGTAGSGDVLAGIIAAERAAGKEPFEAACAGVWRHAEAARRAGPKMIADDLIPESDKYRRGVVGVAAGSERYPGAAVLAVAGALRGGAGAVRYVGPPAEAVLARFPEVLIGPGRVQAWVAGSGLGDAPEARERLARALDDGVSTVVDADGLRMLGPAPGRTARGPLVLTPHAGEAAALLGVAREEVEEARLESVRQLARQTGATVLLKGSTTLIASPPGEADAGEEDGGTVLANLNGTGWLATAGTGDVLAGLIGSLLAAGLAPADAAAVGAHLHGLADYPRLKGLLVPRARSAHKGRCGRVLLTGGNLGMQGAILLAGQACLRAGAGLVRLCQHPDAPPASLVQPELMGCPAGMDEAWASVHVLGPGLGQDEWARARFEHRLAAPEPLVLDADGLNWLAQAPRHRDNWVLTPHPGEAARLLGCTIDEVEADRFAAVSRLQRRYGGVVLLKGAGSLICDGPTIALCDEGNPGMASGGMGDLLSGIIAALLAQGWPAGMAARLGAVIHGEAADLAAAEGERGMLASDLLPWIRRLVNP",
    "response#3": "MREADAFTIESGSMESIELMERASSAFASKIMERWGKDTPVKIFAGSGNNGGDALAVARMLSQSGYKVSVFLFNINGSLSPDCQKNKERLAECPGLEMTEVTSQFVPPTLDRGDLVVDGLFGTGISRPLNGGFASVVKYINSSHSTVVSIDVPSGLMCEDNSYNFMNHVVRADYTFTFQFPKLAFFFPDNEQYVGEWEVLDIGVKDPETPIAVLEQIGPAAWRNTVGVAAGSERYPGAAVLAVAGALRGGAGAVRYVGPPAEAVLARFPEVLIGPGRVQAWVAGSGLGDAPEARERLARALDDGVSTVVDADGLRMLGPAPGRTARGPLVLTPHAGEAAALLGVAREEVEEARLESVRQLARQTGATVLLKGSTTLIASPPGEADAGEEDGGTVLANLNGTGWLATAGTGDVLAGLIGSLLAAGLAPADAAAVGAHLHGLAAESDKYRRGVLGVAAGSHLYTGAAVLAVGGALRAGAGMVRYAGAEEPVAQVRAHWPEAVITVLDRPSIDEVGRVQAWVLGPGLGTGERAHDLAASVLASDVPVLIDADGLTIVAKDRELLRRTAPVLLTPHAGELARLTGAERADIEAARLEHVRAAAAELGVTVLLKGSTTLIAGPSGQARVNVTGSSWLATGGTGDVLSGVAGALLAQGLSCYDAAACGAYVHGLAGAP"
  },
  {
    "reference": "MKASQFFISTLKEAPADAEVVSHQLMMRAGLIKKLGAGIYNYMPMGLRVIRKVEAIVREEMNRAGAVECTMPVVQPAELWQETGRFDKMGPELLRIHDRHGRDFVIQPTSEEVVTDIARQELRSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFIMKDAYSFDRDQAGAKASYQVMAQAYRRIFDRFGLRYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPASDYAANMEKAEALAPAGARPAAKQPLTVTPTPGKSTCADVAELLSVPLSTTVKSLVLATDETDAAGEIIRSQVWLLLLRGDHDMNEIKVAKVPGLDAGFRFATVAEIADHFGCKPGYLGPLNLQKPVKLVVDRDVAVMADWICGANQEGHHITGVNWGRDLPEPDMVADLRNVVAGDLSPDGQGELAIERGIEVGHVFYLGTKYSKAMNATFLGEDGKPAFFEMGCYGIGVTRLPAAAIEQNHDERGIIWPDAIAPFTVVICPVGMDRSEAVKAQAESLYADLLAAGVDVILDDRGERPGAMFADWELIGVPHRVTIGDKSLKEGQVEYQHRRDASATKVGVADILAHVKERLAA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Catalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro); One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP; The misacylated Cys-tRNA(Pro) is not edited by ProRS.; As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine; The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala- tRNA(Pro)2. The designed protein should be involved in prolyl-tRNA aminoacylation by localizing to cytoplasm and exhibiting aminoacyl-tRNA editing activity, ATP binding, proline-tRNA ligase activity. The designed protein sequence is ",
    "response#1": "MSEKLNTPLINQIQTFLIQTKINDITSIAALLIRGDHELNFFKVEKIDIINKPLVFLNEKEVISLIGVKKEFLGPLGLKVPIIADISTFNMKNFTIGSNINKHFFINVNWTPNVHTIEELSKFLDVHPSKIMKAVLYIVNEKEPVLVLIRGDREIDENKLEKVLGTDNFRLATDEEVQELLGTKKGFIGIFNLPENIKVLWDNSLYGVKNLVVALNEPDWHYINVNTPHAKTIEELVEFFGVDSKKFVKTIIYRAENKVVAVMVRGDREVNETKVKNAVGSVDVELADEKTVKKATGAEVGFAGPIGLDVDYLLIDNEVTYMYNFIVGANETGYHYANANYDKGTPNVHTIEELSKFLDVHPSKIMKAVLYIVNEKEPVLVLIRGDREIDENKLEKVLGTDNFRLATDEEVQELLGTKKGFIGIFNLPENIKVLWDNSLYGVKNLVVALNEPDWHYINVNYDKGQSIDKTALYPKFRLKDRHNRDFALGPTHEEIITTLVDADVHSYRHLPLLLYQIQNKYRDEIRPRFGLMRGREFIMKDLYSFDIDEEGLDISYHKMYEAYNRIFQRLKLQYRVVEADSGAIGGNESHEFMVLAENGEAEIVYCKNCDYGANTEKAVCSLEEPKVTEEEQLELEKVHTPGQRTIQDLVDYMNIPKEKQIKTLIYYADEELVAAIVRGDRELNEIKFKNVLGCNQLFMADETAVKQLCSAGFGSLGPVGLPLKTYIDLEVSQMKNFACGANEDDYHFINVNLGRDFTPAGINDIRNAVAGDSCPVCSAPLYSMRGIEVGHIFKLGTKYSEVLEANYLDQKGQEKPMVMGCYGIGISRTMAAAVE",
    "response#2": "FRIQDRHDLSYALGPTHEESFSFLLKPLLKSYKDLPVNVYQIQTKFRDEIRPRFGVIRSREFIMKDAYSFHIDDSSLDDTYQAMRVAYRKIFDRCGLKTIPVQADSGSMGGSASEEFMVVSPIGEETLLLCNSCGYSSNSEKTPLILKKENSSAKFSEKKEISTPGKKTISEVSTLLGVSESETIKAVALKSEKKKILVFLRGDLELNLHKLHSLLKIADSEPMTDLEIRELGLIPGFISPIAPNDKIKVLYDRSLQKDFPYVVGSSKEDFHTQGFILEKEISGLPEFADVALAREGDLCPNCSFPLKAEKGIEVGHIFKLGDKYTKAFGIQVLDQNGKSKTLTMGCYGIGVNRTMATVIEPYHVIIVPVNVSNEAQNRVAEDIYAALQKEGIEVLIDDRDLRAGVKFNDADLLGIPIRITVGKKVDDGIVEIKLRENEEAEEVKISDVVEKVPFEVAIIPVAMNDEAIVSTSQEIYDYFLKEGIDVIIDDREVSAGFKFKDIDLIGVPIKIIIGKRLKEGKIELKQRDKEEGTLIEFKNVKEVAIIPVAMNDEAIVSTSQEIYDYFLKEGIDVIIDDREVSAGFKFKDIDLIGVPIKIIIGKRLKEGKIELKQRDKEEGTLIEFKNVKDAKTIRAVAERLSVQEEKIIKSLLVSIDDEWALILLRGDHELNDIKLKHALGASDIRLATEEEVLEVIGTEVGTIGPVDVKGVKVIADHGIKSIVNGVCGANESNKHFIHVNEPFSVGILPMNMHKSHRVTDIAEQLYKDLNEAGIDVLLDDRKERPGVMFADMELIGIPHTVVIGDRNIDAGVFEYKNRRTGEKQDIPFDQLLDFL",
    "response#3": "EAEVLSHQLLLRAGFIRKATAGVYSYLPLANRVLKKIMNIVREEMDRAGGQEVILPIIQPAELWKKSGRWEVYGDEMFRLKDRHNRDFALGPTHEEIITTLVDADVHSYRHLPLLLYQIQNKYRDEIRPRFGLMRGREFIMKDLYSFDIDEEGLDISYHKMYEAYNRIFQRLKLQYRVVEADSGAIGGNESHEFMVLAENGEAEIVYCKNCDYGANTEKAVCSLEEPKVTEEEQLELEKVHTPGQRTIQDLVDYMNIPKEKQIKTLIYYADEELVAAIVRGDRELNEIKFKNVLGCNQLFMADETAVKQLCSAGFGSLGPVGLPLKTYIDLEVSQMKNFACGANEDDYHFINVNLGRDFTPAGINDIRNAVAGDSCPVCSAPLYSMRGIEVGHIFKLGTKYSEVLEANYLDQKGQEKPMVMGCYGIGISRTMAAAVEGLYTWMPMGLRALRKAEAIVREEMNKAGALEVLMPAIQPAELWQESGRWEQYGPELLRLKDRHDREFCVGPTHEEVITDLARNELNSYKQLPINFYQIQTKFRDEIRPRFGLMRGREFLMKDAYSFHLNQESLQETYDRMHQAYCNIFTRLGLNFRPVQADTGSIGGTGSHEFHVLAESGEDDIAFSDSSDYAANIEKAEAIPRETERAAPGEELRLIDTPDAKTIIELVEQFGVAVEKTVKTLVVHGVEKGQLVALIVRGDHELNEIKAANLAQVASPLVFASEAEIRAAIGAGPGSLGPLNLPIPCVIDRSVALMSDFSAGANIDDKHYLGLNWERDLPLPQVADLRNVVAGDPSPDGKGSLVIKRGIEVGHIFQLGTKYSEAMNCQVMGENGKPATLIMGCYGIGVSRVVAAAIEQNYDERGILW"
  },
  {
    "reference": "MRIAVVGATGMVGEVMLQILKERNFPISELLLVASEKSVGKKIPFNGKEHSVISLADAVTAKPDIALFSAGGETSLEWAPKFAAAGTTVIDNSSAWRMDPTKKLIVPEINASSLNASDKIIANPNCSTIQLVMTLAPLHETYGIKRVVISTYQSITGTGVKAVQQLENEYNNTKGEMAYPYPIHQNAIPQCDSFEENGYTKEEMKLVRETQKILNDKTIAVTATAIRIPVVGGHSESVNVQFHNDFSVSDIRNILQQTSGVVVQDNPDVSTYPMPIYAKGKDDVFVGRIRRDESQPNTLNMWIVSDNLRKGAATNAIQIAEYLVSQNILQKK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must have a Acyl-thioester intermediate active site that is highly conserved among related enzymes.2. A protein with protein dimerization activity functions is required to enable threonine biosynthetic process. The designed protein sequence is ",
    "response#1": "GLLRQDLVEWVSAMTYQAASGAGAQNMRELLQQMGALHDAVRAELAEPASAILEIDRKVAKSMRSPDFPTRNFRNTALAGSLIPWIDVPVEGGQSKEEWKGGAECNKILGRPAFRTPGSIPVDGLCVRIGAMRCHAQGLTIKLKRDVPLTELGEIIAQANPWVKVVPNEREITERELTPAAVTGTLTIPVGRLHKLAMGPEYLGAFTVGDQLLR",
    "response#2": "LGGLFAEDLVEWVSPMTYQAASGSGAKHMRELITQMGEINEAVANQLDNPASAILEIDKLVAEKMRSDEFSTDQFGVPLAGSLIPYIDTQLPSGQSREEWKAGAETNKILGTQDQAIPIDGLCVRVGAMRCHSQALTIKLKKDLPVEEIEAKLAAANDWVKVIPNDREASINELSPTVATGTLSIPVGRIRKLEMGPEYISAFTVGDQLLK",
    "response#3": "LNGLFREGLIEWITAMTYQAASGAGAQNMRELLAQMGAVHDSVRGLLADPASAILEIDGKVAQTMRSSAFPTRHFGVPLAGSLIPWIDKDLGNGTSREEWKGDAECNKILGLPAQGEGHIPIESLCVRVGAMRCHSQALTIKLRKDVPLADVEALIAEANDWVKLVPNDRESSVRGLSPAAVTGTMSVPVGRLRKLAMGPEYLSAFTVGDQLLY"
  },
  {
    "reference": "MLQRDTTIINKLGLHARASAKLTQLAGNFVSQVKMSRNGRQVDAKSIMGVMMLAAGIGSTVTLETDGPDEQEAMDALLALIANRFGEGE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS); This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane2. The protein design should aim to mimic or enhance the function of natural proteins involved in phosphoenolpyruvate-dependent sugar phosphotransferase system, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "MLEHELTVTNKLGLHARATAKLVQTMSKFQSNTTLSTKGREVNAKSIMGVMLLAASQGTVIRVRIDGEDEHTAMQALSELFENRFNER",
    "response#2": "MERTVTVVPEDGLHARPASKFVETANKFDADVQLGRADEDDLVPAASMLAVTGLGVGHDESVRLVAEGDDAEAALDALEDILSTPEAKR",
    "response#3": "MPALEITIINKLGLHARAAAKFVGVAGRYPCQVRVGRNPESCVDGKSIMAVMMLAAGKGTNLHLHTEGEQEDEALNALVELINNRFDER"
  },
  {
    "reference": "MDSIATNTHSSSIVNAYNNNPTDVVKTQNIKNYTPKVPYMCVIA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in positive regulation of pheromone response MAPK cascade, potentially with improved properties such as stability, specificity or selectivity.2. For general function, the protein need meet that All three mfm genes contribute to the production of M-factor. The designed protein sequence is ",
    "response#1": "VMEGPLFLQSQRFGTKRWRKTWAVLYPASPHGVARLEFFDHKGSSSRGGRGGSRRLDCKMIRLAECVSVVPVTVESPPEPGAVAFRLDTAQRSHLLAADAVSSTAWVQTLCRC",
    "response#2": "KKVRPIGSGNFSTVLLYELMDQSNPKLKQVAVKRLKYPEELSNVEQINTSLRYKETLSRLENSLTRELQVLKSLNHPCIVKLLGINNPIFVTSKKPLCDLIIKTPRALPPCDMIMSYCPAGDLLAAVMARNGRLEAWLIQRIFTEVVLAVKYLHENSIIHRDLKLENILLKYSFDDINSFRDSPIYCKQNFIELADFGLCKKIENNEMCTARCGSEDYVSPEILMGVPYDGHLSDTWALGVILYSLFEDRLPFDPPPNASARQRSRATSHRIARFDWRWYRLSDYKTNVGKQIVENTLTRKNQRWSINEIYESPFY",
    "response#3": "LKGMLEVFRELTDVRRYWVDVTVAPNNISCAVISEDKRQVSSPKPQIIYGARGTRYQTFVNFNYCTGILGSQSITSGKHYWEVDVSKKTAWILGVCAGFQPDAMCNIEKNENYQPKYGYWVIGLEEGVKCSAFQDSSFHTPSVPFIVPLSVIICPDRVGVFLDYEACTVSFFNITNHGFLIYKFSHCSFSQPVFPYLNPRKCGVPMTLCSPSD"
  },
  {
    "reference": "MTADRWAGRTVLVTGALGFIGSHFVRQLDARGAEVLALYRTERPEIQAELAALNRVRLVRTELRDESDVRGAFKYLAPSIDTVVHCAAMDGNAQFKLERSAEILDSNQRTISNLLNCVRDFGVGEVVVMSSSELYSASPTVAAREEDDFRRSMRYTDNGYVLSKTYGEILARLHREQFGTNVFLVRPGNVYGPGDGFDCSRGRVIPSMLAKADAGEEIEIWGDGSQTRSFVHVADLVRASLRLLETGKYPEMNVAGAEQVSILELAGMVMAVLGRPERIRLDPSRPVGAPSRLLDLSRMSEVIDFDPQPLRAGLEETARWYRLHKR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The NAD(+) binding site should be located in a region of the protein that is accessible to the ligand.2. For general function, the protein need meet that Cannot directly reduce dTDP- 4-dehydro-6-deoxyglucose, and thus acts after the epimerization step catalyzed by GerF.; Catalyzes the stereospecific reduction of the C-4 keto group of dTDP-4-dehydro-6-deoxy-D-allose, leading to dTDP-6-deoxy-D-allose, an intermediate in the biosynthesis of the mycinose moiety of dihydrochalcomycin (GERI-155) antibiotic3. The protein with the reaction dTDP-6-deoxy-alpha-D-allose + NADP(+) = dTDP-4-dehydro-6-   deoxy-alpha-D-allose + H(+) + NADPH, the Proton donor/acceptor must be optimized to ensure efficient catalysis.4. The protein design should aim to mimic or enhance the function of natural proteins involved in carbohydrate metabolic process, antibiotic biosynthetic process, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "DAADVAAAWPLPGPADDKYTQGVTGVAAGSATYPGAAVLAAAAAVRATAGMVRYAGPAADVVRSHWPEVVATGSITDAGRVQAWVVGPGIGTGRQGRDQLAYALAQGVPVCADADAITLIAAHPDVLDARDPATPLLLTPHDGEFARLTGSAPGPDRVGAVRRAARRLGAVVLLKGHRTLVAEPGGRVVVNRPRGAWLATAGSGDVLSGVIGALLASGLEPWLAAAVGADVHARAGELAARGAPTSASGVLAAIPDAIRQDAADVAAAWPLPGPADDKYTQGVTGVAAGSATYPGAAVLAAAAAVRATAGMVRYAGPAADVVRSHWPEVVATGSITDAGRVQAWVVGPGIGTGRQGRDQLAYALAQGVPVCADADAITLIAAHPDVLDARDPATPLLLTPHDGEFARLTGSAPGPDRVGAVRRAARRLGAVVLLKGHRTLVAEPGGRVVVNRPRGAWLATAGSGDVLSGVIGALLASGLEPWLAAAVGADVHARAGELAARGAPTSASGVLAAIPDAIRQARADDKYTQGVTGVAAGSATYPGAAVLAAAAAVRATAGMVRYAGPAADVVRSHWPEVVATGSITDAGRVQAWVVGPGIGTGRQGRDQLAYALAQGVPVCADADAITLIAAHPDVLDARDPATPLLLTPHDGEFARLTGSAPGPDRVGAVRRAARRLGAVVLLKGHRTLVAEPGGRVVVNRPRGAWLATAGSGDVLSGVIGALLASGLEPWLAAAVGADVHARAGELAADL",
    "response#2": "DAADVAAAWPLPGPADDKYTQGVTGVAAGSATYPGAAVLAAAAAVRATAGMVRYAGPAADVVRSHWPEVVATGSITDAGRVQAWVVGPGIGTGRQGRDQLAYALAQGVPVCADADAITLIAAHPDVLDARDPATPLLLTPHDGEFARLTGSAPGPDRVGAVRRAARRLGAVVLLKGHRTLVAEPGGRVVVNRPRGAWLATAGSGDVLSGVIGALLASGLEPWLAAAVGADVHARAGELAARGAPTSASGVLAAIPDAIRQARAALAPQPVRDAADVAAAWPLPGPADDKYTQGVTGVAAGSATYPGAAVLAAAAAVRATAGMVRYAGPAADVVRSHWPEVVATGSITDAGRVQAWVVGPGIGTGRQGRDQLAYALAQGVPVCADADAITLIAAHPDVLDARDPATPLLLTPHDGEFARLTGSAPGPDRVGAVRRAARRLGAVVLLKGHRTLVAEPGGRVVVNRPRGAWLATAGSGDVLSGVIGALLASGLEPWLAAAVGADVHARAGELAARGAPTSASGVLAAIPDAIRQARDAADVAAAWPLPGPADDKYTQGVTGVAAGSATYPGAAVLAAAAAVRATAGMVRYAGPAADVVRSHWPEVVATGSITDAGRVQAWVVGPGIGTGRQGRDQLAYALAQGVPVCADADAITLIAAHPDVLDARDPATPLLLTPHDGEFARLTGSAPGPDRVGAVRRAARRLGAVVLLKGHRTLVAEPGGRVVVNRPRGAWLATAGSGDVLSGVIGALLASGLEPWLAAAVGADVHARAGELAARGAPTSASGVLAAIPDAIRQARL",
    "response#3": "VAAGSATYPGAAVLAAAAAVRATAGMVRYAGPAADVVRSHWPEVVATGSITDAGRVQAWVVGPGIGTGRQGRDQLAYALAQGVPVCADADAITLIAAHPDVLDARDPATPLLLTPHDGEFARLTGSAPGPDRVGAVRRAARRLGAVVLLKGHRTLVAEPGGRVVVNRPRGAWLATAGSGDVLSGVIGALLASGLEPWLAAAVGADVHARAGELAADAADVAAAWPLPGPADDKYTQGVTGVAAGSATYPGAAVLAAAAAVRATAGMVRYAGPAADVVRSHWPEVVATGSITDAGRVQAWVVGPGIGTGRQGRDQLAYALAQGVPVCADADAITLIAAHPDVLDARDPATPLLLTPHDGEFARLTGSAPGPDRVGAVRRAARRLGAVVLLKGHRTLVAEPGGRVVVNRPRGAWLATAGSGDVLSGVIGALLASGLEPWLAAAVGADVHARAGELAARGAPTSASGVLAAIPDAIRQARDAADVAAAWPLPGPADDKYTQGVTGVAAGSATYPGAAVLAAAAAVRATAGMVRYAGPAADVVRSHWPEVVATGSITDAGRVQAWVVGPGIGTGRQGRDQLAYALAQGVPVCADADAITLIAAHPDVLDARDPATPLLLTPHDGEFARLTGSAPGPDRVGAVRRAARRLGAVVLLKGHRTLVAEPGGRVVVNRPRGAWLATAGSGDVLSGVIGALLASGLEPWLAAAVGADVHARAGELAARGAPTSASGVLAAIPDAIRQARADDKYTQGVTGVAAGSATYPGAAVLAAAAAVRATAGMVRYAGPAADVVRSHWPEVVATGSITDAGRVQAWVVGPGIGTGRQGRDQLAYALAQGVPVCADADAITLIAAHPDVLDARDPATPLLLTPHDGEFARLTGSAPGPDRVGAVRRAARRLGAVVLLKGHRTLVAEPGGRVVVNRPRGAWLATAGSGDVLSGVIGALLASGLEPWLAAAVGADVHARAGELAA"
  },
  {
    "reference": "MAFVVRFIKGVRNHWKKSTFAAFALAYGANYAKEYYDVQELMRVYCQEAAAYGNAPIGSNENPRRITIQKDTQKPRGGFTRTDAIVVAGGDGTLSEVITGLMRKSESNPLICLLFRSVAVRKTNTIVRFIKGVRNHWKKSTFAAFALAYGANYAKEYYDVQELMRVYCQEAAAYGNAPIGSNENPRRITVILNPNSNKRKAMKEYEKYCSPILHLAGVFVDVIQTDSEGHAKSLVEDLPGTDAIVVAGGDGTLSEVITGLMRKSESNSLNLPIGVLPLGKTNTIGKSFFPGGDYLQNVRRLADASLAIIKEHTKPIDVMKIEVLQDDELKGKTVYAVSSIEWGPYRDAQAKVDKYWYFGPLRKYVTYLFNGYKDSLSWSCKASLNYTLPCEGCKNCVAIPQIKETLRWYHKVLPLRQHKLPNPVPLIINDFCQETHRKDVSTSDFAIATTNTLQNVNTIPKLTVNLGPNSVDYFNFVTEGWRTEVGRERSVVEVIQAKQIEVVPNEEKKVQWFSIDSEEFEVKPVKITLLPKAALMFYLPNRLLNV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein have enhanced an N-acylsphing-4-enine + ATP = ADP + an N-acylsphing-4-enine   1-phosphate + H(+).2. The presence of DAGKc domains is critical for the interaction of the designed protein with its intended targets.3. A protein that can modulate the overall flux of metabolites through the Lipid metabolism, glycerolipid metabolism pathway, enhancing or suppressing its activity as needed.4. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.5. A protein that can perform kinase activity, metal ion binding in mitochondrial inner membrane, mitochondrial intermembrane space to support glycerolipid metabolic process. The designed protein sequence is ",
    "response#1": "MIGLTAGAYSEAEKKKKKYWYFGPLKFKWAYIRSTLTQWPSVISGKISYIPATEENTKLTKIVIKQPVPEVRRSWWNILYLFYKPRPQHSQEVYEEVEAEDGQDEETISEDVSSMELSVIAPIAQGQQEKIVAPELHIGPSQINRQDFISEGFRREKLPTRVFNSPDDRHSFVKKFKFVPDLPEDAEKKVWYSIDGEQYEAVPVEIKLLRNRLNIFQSS",
    "response#2": "MPLKKATVFLNPAACKGKAGNLFEKNAAPILHLSGLDVTVVKTDFEGQAKKLLELMESTDLIIIAGGDGTVQEVITGLLRRADEATFSKIPIGFIPLGKTCTLSHTLYPESMSQVQHITNATLAILKGETVPLDVLQIKGEVSGLRWGAYRDAGVKVSKYWYLGPLKTQAAHFFSTFKEWPQKHQAALMYLGPTERHPEEPDEKSRPPCFVRLYRRLLAFLSSPKGSSQEAAPEDWEELKLSTIELSIATRNWHLDLTRTEDFMDICIEAESVSKGQFVHRGSQKMRYPHMCPEGTQYIQASRCILQLPEGTEGSFGIDNEEYEAMPVEVTGLRWGAYRDASSKLSKFWYLGPLKDKACHLFSMYKEWPQVYRASLAYLGPTERPPEESDEKPPRPGLWRRILRRLELYFSSPKEAEQVPMEPEHWDEVEASAVEVTISTLNKQMDLTVRPRGSWAALPLTCFGLHDTVFRREGFIGSIKLPKSPSMSQSRPFHLQECGTEGFFSIDNEDYEAMSVDVKLLPRKLRFFCDSIKKQQMV",
    "response#3": "MIGLTAGAYSEAEKKKKKYWYFGPLKFKWAYIRSTLTQWPSVISGKISYIPATEENTKLTKIVIKQPVPEVRRSWWNILYLFYKPRPQHSQEVYEEVEAEDGQDEETISEDVSSMELSVIAPIAQGQQEKIVAPELHIGPSQINRQDFISEGFRREKLPTRVFNSPDDRHSFVKKFKFVPDLPEDAEKKVWYSIDGEQYEAVPVEIKLLRNRLNIFQSL"
  },
  {
    "reference": "MSEPKPIQDTLDRRHSRESSISSASTTSLVFDRLAEESEKNHDASSRPHPSAARHAYTDDNSDAIKESDINDPETGPFLGASSETTPPRKGVDRKLKKVLLIVGGFFVAAWIVSLVVFLTNKSYKHGSQIDHDPAATNRKSGKRVTLDQVQSGFWRPTSHTFSWIPGPDGEDGLLLEQEARGKHFLVVEDVRSQGSADGEAHPDAAESRTLIKDPWFYWGDQQHSILQTWPSKNQKKVLVATQKQRNWRHSFTALYWVFDVESQSAEPLDPAHPEERVQLATWNAQSDAIVFTRSNNLFLRKLADDKVTPITTDGGPEYFYGIPDWVYEEEVFSGNSATWWSADGKHVAFLRTNETEVPEYPIQYFVSRPSGAEPEVGEENYPEVRQIKYPKVGSPNPVVDLLFYDVEKGDVFTVDIDGDFPEKDKLINFVMWADGNVLVKTTNRVSDVLQVNLIDIVARTGKTVQHVDVAKIDGGWFEISHVMYIPADPKNGRPHDGYVDTVIHNDGDHLAYFTPMDNPKPVYITEGPGWEVDGSASAVDLKNNLVYFRSTKESSIQRHIYSVHLNGTDMKPFTDTTHESYYDVSFSSGAGFGLLSYQGPKVPWQKVVSTPSNPKSYERIIEENKDLTQQAKKHELPVLEYGTIKVDDVELNYVERRPPHFDKNKKYPVLFQQYSGPGSQTVTKKFAVDFQSSDGGRRTTRSPRRATGRPSATSTPTAFAIWGWSYGGFATLKTLETDAGRTFRYGMAVAPVTDWRFYDSIYTERYM",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should exhibit Basic and acidic residues compositional bias.2. The protein can be expressed in vacuolar membrane and perform serine-type peptidase activity, aminopeptidase activity without triggering undesirable cellular responses.3. We need a protein that can perform Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a   polypeptide, preferentially when Yaa is Pro, provided Zaa is neither   Pro nor hydroxyproline. with high efficiency.4. The protein should have Helical; Signal-anchor for type II membrane protein domains to ensure proper insertion into the membrane.5. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "VNGFPCKNSEGYCFMGKCPTREDQCSELFDDEAIESHDICYKMNTKGNKFGYCKNKENRFLPCEEKDVRCGKIYCTGGELSSLLGEDKTYHLKDPQKNATVKCKTIFLYHDSTDIGLVASGTKCGEGMVCNNGECLNNGFPCKNSEGYCFMGKCPTREDQCSELFDDEAIESHDICYKMNTKGNKFGYCKNKENRFLPCEEKDVRCGKIYCTGGELSSLLGEDKTYHLKDPQKNATVKCKTIFLYHDCAVNGFPCKNSEGYCFMGKCPTREDQCSELFDDEAIESHDICYKMNTKGNKFGYCKNKENRFLPCEEKDVRCGKIYCTGGELSSLLGEDKTYHLKDPQKNATVKCKTIFLYHDSTDIGLVASGTKCGEGMVCNNGECLNVNGFPCKNSEGYCFMGKCPTREDQCSELFDDEAIESHDICYKMNTKGNKFGYCKNKENRFLPCEEKDVRCGKIYCTGGELSSLLGEDKTYHLKDPQKNATVKCKTIFLYHDSTDIGLVASGTKCGEGMVCNNGECLNCKIAVVNVEGTIIDGESDEENAGGDTIARILRKAHDDNSVKAVILRVNSPGGSAFASEIIRQETENLQKIGKPVIVSMGAMAASGGYWISSTADYIIADSNTITGSIGIFTMFPTFENSIKKIGVHADGVSTTELANTSAFSPLAKPVQDIYQTEIEHGYDRFLEIVSKGRQLSKTQVDKLAQGQVWLGSDAFQNGLVDEIGSFNEAVNKAEQ",
    "response#2": "FKDAGKPVIAYADNYSQGQYYLASFADEIYLNSIGSVDIHGLSQENLYFKEMLDKLAVTPHIFRVGTYKSAVEPFLRNDMSAEAKANMQRWLGEMWNNYVLSVSENRNIKKDRILPNAKQYLAELKALKGNSTAYAQQRGLVTDVVTRLDLDKKQ",
    "response#3": "FKDAGKPVIAYADNYSQGQYYLASFADEIYLNSIGSVDIHGLSQENLYFKEMLDKLAVTPHIFRVGTYKSAVEPFLRNDMSAEAKANMQRWLGEMWNNYVLSVSENRNIKKDRILPNAKQYLAELKALKGNSTAYAQQRGLVTDVVTRLDLDKKK"
  },
  {
    "reference": "MRVKETQMNWPNLWTWGTLILGLVIMCSASDNLWVTVYYGVPVWRDADTTLFCASDAKAHDTEVHNVWATYACVPTDPNPQEIPLENVTENFNMWKNKMVEQMQEDVISLWDQGLKPCVKLTPLCVTLNCTTANLINTNNNTATVSNGIGNLTDEVRNCSFNMTTLLSDKKKKVYALFYKLDIVPITDSNGSEYRLINCNTSVIKQACPKINFDPIPIHYCTPAGYALLKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNVKTIIVHLNKSVEINCTRPSNNTRTSVPMGPGRTFYRTGDIIGDIRQAYCEINGAKWNETLRQVAEKLKEHFNNKTIKFQPPSGGDLEITMHHFNCRGEFFYCNTTKLFNSTWTGNENMTEGNDTIILPCKIKQIINMWQGTGQAMYAPPISGIINCVSNITGILLTRDGGNNETNGTETFRPGGGNIKDNWRSELYKYKVVQIEPLGVAPTRAKRRVVDREKRAVGIGAMIFGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKIICTTAVPWNSSWSNKTYEDIWNNMTWIEWEREISNYTDTIYELLEDSQNQQDRNEKDLLELDKWKNLWNWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSVVNRVRQGYSPLSLQTLIPNPGGPDRLGGIEEEGGEQGRDRSVRLVNGFLALAWDDLRNLCLFCYHRLR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein contains novel Retroviral envelope protein GP41-like domains that confer a unique function or activity.2. For general function, the protein need meet that The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Transmembrane protein gp41: Acts as a class I viral fusion protein3. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.4. The protein should be optimized for host cell plasma membrane, host cell endosome membrane-specific folding and stability, while maintaining structural molecule activity. The designed protein sequence is ",
    "response#1": "MWVTVYYGVPVWKEATTTLFCASDAKAYDTEAHNVWATHACVPIDPNPQEIPLENVTENFNMWKNNMVEQMHEDIISLWEQSLKPCVKLTPLCVTLHCVNVNITNNSGRNGTTTTTSTPTANPTANTTGSPNTTSSTVTEDGGMTNCSFNTTTYIVNKVKKEYALFYKSDVEPIDNTSSSYTLINCNSSTVTQACPKISFEPIPIHYCTPAGFAILQCNDKEFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVVIRSENFSDNAKTIIVQLNTSVEIKCIRPSNNTRKSIRIGPGRTLYATDRIIGDIRQAHCNISGASWNNTLEQIVTKLREQFGNKTIVFQQPPPGGDPEITLHNFNCGGEFFYCNTSKLFNSTWRTNGTWYNGTWNGENGTLTLPCRIKQIVNMWQKVGKAMYAPPISGKISCTSNITGLLLTRDGGNGNGTSENQTEIFRPTGGEMRDNWRSELYKYKVVRIEPLGIAPTNARRRVVQREKRXLGALGMLFLGVLSAAGSTMGAASLTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNTSWSNKSLNDIWDNMTWMEWDREINNYTNLIYTLLEESQNQQEKNEQELLELDKWASLWSWFDISNWLWYIRIFIMIVGGLVGLRIIFSVLSIVNRVRQGYSPLSFQTRLPTQRGPDRPEGIEEEGGERDRDRSRPL",
    "response#2": "MWVTVYYGVPVWKEATTTLFCASDAKAYDTEAHNVWATHACVPIDPNPQEIPLENVTENFNMWKNNMVEQMHEDIISLWEQSLKPCVKLTPLCVTLHCVNVNITNNSGRNGTTTTTSTPTANPTANTTGSPNTTSSTVTEDGGMTNCSFNTTTYIVNKVKKEYALFYKSDVEPIDNTSSSYTLINCNSSTVTQACPKISFEPIPIHYCTPAGFAILQCNDKEFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVVIRSENFSDNAKTIIVQLNTSVEIKCIRPSNNTRKSIRIGPGRTLYATDRIIGDIRQAHCNISGASWNNTLEQIVTKLREQFGNKTIVFQQPPPGGDPEITLHNFNCGGEFFYCNTSKLFNSTWRTNGTWYNGTWNGENGTLTLPCRIKQIVNMWQKVGKAMYAPPISGKISCTSNITGLLLTRDGGNGNGTSENQTEIFRPTGGEMRDNWRSELYKYKVVRIEPLGIAPTNARRRVVQREKRAGLGAVYLAE",
    "response#3": "MWVTVYYGVPVWKEATTTLFCASDAKAYDTEAHNVWATHACVPIDPNPQEIPLENVTENFNMWKNNMVEQMHEDIISLWEQSLKPCVKLTPLCVTLHCVNVNITNNSGRNGTTTTTSTPTANPTANTTGSPNTTSSTVTEDGGMTNCSFNTTTYIVNKVKKEYALFYKSDVEPIDNTSSSYTLINCNSSTVTQACPKISFEPIPIHYCTPAGFAILQCNDKEFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVVIRSENFSDNAKTIIVQLNTSVEIKCIRPSNNTRKSIRIGPGRTLYATDRIIGDIRQAHCNISGASWNNTLEQIVTKLREQFGNKTIVFQQPPPGGDPEITLHNFNCGGEFFYCNTSKLFNSTWRTNGTWYNGTWNGENGTLTLPCRIKQIVNMWQKVGKAMYAPPISGKISCTSNITGLLLTRDGGNGNGTSENQTEIFRPTGGEMRDNWRSELYKYKVVRIEPLGIAPTNARRRVVQREKRXLGGLGALFIGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGLWGCSGKLICTTNVPWNSSWSNKSQTDIWDNMTWMQWDREINNYTNTIYRLLEESQSQQEKNEKDLLALDNWNNLWNWFSITQWLWYIKIFIMIVGGLIGLRIIFSVLSIVKRVRQGYSPLSFQTLIPTPRGLDRPERIEEEGGEQDRDRSIRL"
  },
  {
    "reference": "MGAMAPRTLLLILAAALAPTQTRAGSHSMRYFDIAVSRPGLGEPRFIIVGYVDDTEFVRFDSDAEKPRYEPRAPWMEPEGPEYWEEQTRRAKEGEQTFRVNLRTALHYYNQSEDRSHTIQRLSGCDVGSDGRLLRGYEQYAYDGRDYIALNEDLKTWTAADFAARISRNKWDQAGVAERLRAYLEGECVEWLRRYLELGKETLLRSDPPKAHVTLHPRPEGDVTLRCWALGFYPADITLTWQLNGEDLTQDMELVETRPAGDGTFQKWASVVVPLGKEQNYTCRVEHEGLPEPLSQRWEPPPSTDSNMETTVIYVVLGAVAIIAAVIIGAVVTVVRKRRRNTGGKGVYTPAPGTDSSQSSDVSLPDCKA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The ideal protein would be one that plays a critical role in regulation of interleukin-12 production, T cell tolerance induction, regulation of interleukin-6 production, regulation of T cell anergy, immune response through signaling receptor binding, protein-containing complex binding, TAP binding, chaperone binding within Golgi apparatus, extracellular space, cell surface, phagocytic vesicle membrane.2. For general function, the protein need meet that Involved in the presentation of foreign antigens to the immune system. The designed protein sequence is ",
    "response#1": "GSHSLRYFHTAVSRPGLREPRFIIVGYVDHTEFVRYDSDAENPRYEPRARWMEREGPEYWEQQTQIVKGHEWSFRVSLRNLLGYYNQSEGGSHTLQVMSGCDMGSDRSLRRGYEQYAYDGRDYISLNEDLKTWSAADLAAQITLNKWDQAGELDYYKAYLEGPCLEWLLRYLEHGKETL",
    "response#2": "QPTEMTATEGAIVQINCTYQTSGFNGLFWYQQHAGEAPTFLSYNVLDGLEEKGRFSSFLSRSKGYSYLLLKELQMKDSASYLCS",
    "response#3": "GSHSLRYFHTAVSRPGLREPRFIIVGYVDHTEFVRYDSDAENPRYEPRARWMEREGPEYWEQQTQIVKGHEWSFRVSLRNLLGYYNQSEGGSHTLQVMSGCDMGSDRSLRRGYEQYAYDGRDYISLNEDLKTWSAADLAAQITLNKWDQAGELDYYKAYLEGPCLEWLLRYLEHGKETR"
  },
  {
    "reference": "MVDKSRYQKMFPVSWEQLHRDCRALSWRLVDKGPWKGILAITRGGLVPAAIIARELDIHLIDTICLSSYDWKNQGEATILKEVDNDGEGWLLIDDLVDTGKTAKIARDMVPRAHFATVYAKPAGRPTVDTFITEVSQDTWILFPWDAGSQFVEPIAQASE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Ideal protein should have a binding affinity for key enzymes in the XMP from xanthine: step 1/1, XMP biosynthesis via salvage pathway, Purine metabolism pathway to enhance the overall efficiency of the pathway.2. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.3. A protein that contributes to XMP salvage, GMP salvage, purine ribonucleoside salvage by functioning in plasma membrane with magnesium ion binding.4. For general function, the protein need meet that Purine salvage pathway enzyme that catalyzes the transfer of the ribosyl-5-phosphate group from 5-phospho-alpha-D-ribose 1- diphosphate (PRPP) to the N9 position of the 6-oxopurines guanine and xanthine to form the corresponding ribonucleotides GMP (guanosine 5'- monophosphate) and XMP (xanthosine 5'-monophosphate), with the release of PPi5. The designed protein should have a binding site that is specific to the target xanthine, GMP, Mg(2+), 5-phospho-alpha-D-ribose 1-diphosphate ligand. The designed protein sequence is ",
    "response#1": "MPFHPTRRRLLAASALGAAHLVLDDAFAQPLAPLAGHPSTVLSPASATTLHTELGTPYPLVVIGGFHGRTSRSRHLELLRTLSDRSGALFAPMGDGSGGASSPERLAAFSRALAWRLDGKGPDNGAWKAIVAITRGGMAPAMIVARELDIRLVDTISIKSYDHQAQGDAQVLSKPDEAMVGDGTGILVIDDLVDSGKTLEIVRQMYPKAHFATVYAKPKGEPMDPRAAELAGLSGVDLHIVAVTRGGLIPAALIARELDIRLIDTVCIKSYEHMEQSGINVLKGIDHDGDGWLLVDDLVDTGKTAGAVREMLPRAHFVTIYAKPEGRPMLDGISIEPAPRPRRLSHPLEGPYFKPSSPQRADLVEPEGKARLVELSGQVFTRSCRPVGQALVDLWHADERGQYDNAGFRYRGHLFTDEEGRYRFRTILPALYTGRTRHYHVKVQAPRQNLLTTQLYFPDEPMNRRDGLFRAVLRAKKL",
    "response#2": "MNISWDQFHRDARALAWRLAGLNGGQWKAIVCITRGGLVPAAIISRELGIRVIETVSVASYHDYTSQGELNVLKEVTPSLTENDGENVLIVDDLTDTGKTAAVVRAMLPKAHFAAVYAKPKGKPMVDTYITEVSQDTWIYFPWDMGFVPQEISTKEKIQEGPYFKPSSPQRADLVEPEGKARLVELSGQVFTRSCRPVGQALVDLWHADERGQYDNAGFRYRGHLFTDEEGRYRFRTILPALYTGRTRHYHVKVQAPRQNLLTTQLYFPDEPMNRRDGLFRAVLVDEKP",
    "response#3": "MPFHPTRRRLLAASALGAAHLVLDDAFAQPLAPLAAALGAAPA"
  },
  {
    "reference": "MSLLRLTFLGTSAAQPTLHRNLSGLAVKAHSDLLLFDCGEGSQRQMVRYGTGFTVDAVFFTHFHADHYLGIIGFLRTLGMTGRSEPIHLYGPPSAKRLLHQAVHLGVESMSFPVEIHELKDGDVVPRKGYAVHAVGVDHRINALGYALVEDDRPGRFNLDVARSLGVPEGPSFGKLQRGEPVTLEDGRTVKPEDVLGAPRPGRRLVISGDTRPCPALVKAAKDADLLVHESTFSDDEQERAVETRHSTAREAARVAREAGARRLVLTHLSSRHDTDPSKLLTQAREEYQGPVEVAFDGFTVELPLRD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein requires Zn(2+) to activate a critical catalytic site.2. The designed protein should have a binding site that is specific to the target Zn(2+) ligand.3. For general function, the protein need meet that Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity4. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for Endonucleolytic cleavage of RNA, removing extra 3' nucleotides   from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy   group is left at the tRNA terminus and a 5'-phosphoryl group is left   at the trailer molecule.. The designed protein sequence is ",
    "response#1": "FLSATLINYRGRKILVDCGEGTQVSMRISNTGFKSIDIICITHIHGDHIVGLPGLLGTIGNSGRTEPITIIGPEGIRETVEKLRVIANWLPYEINIIENPKDTFKLFDGNINLDVDISVLELDHSSPCIGYCFYFKRQPKFDVEKAEKNKVPKILWNRLQRNEENIVLDGALYTNDMVTGE",
    "response#2": "LFDCGEGTQHQFLRSDLRLSQLRRVFITHMHGDHVFGLPGLLASLGLAGSSSAGVDLYGPDPLVKAD",
    "response#3": "FLSATLINYRGRKILVDCGEGTQVSMRISNTGFKSIDIICITHIHGDHIVGLPGLLGTIGNSGRTEPITIIGPEGIRETVEKLRVIANWLPYEINIIENPKDTFKLFDGNINLDVDISVLELDHSSPCIGYCFYFKRQPKFDVEKAEKNKVPKILWNRLQRNEENIVLDGALYTNDMVTGRR"
  },
  {
    "reference": "MLEKDIKKILFSQEDIVTKTKELGQQLTKDYAGKNPLLVCVLKGAVPFMAELIKHIDTHIEMDFMVVSSYGGGTVSSGEVKILKDVDTNIEGRDVIFIEDIIDTGRTLLYLRDMFKYRKASSVKIATLFDKPEGRVVDIEADYVCYDVPNEFIVGFGLDYDEKYRNLPYVGVLKEEIYNK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for diphosphate + GMP = 5-phospho-alpha-D-ribose 1-diphosphate +   guanine.2. The protein should be able to bind Mg(2+), GMP ligand in a variety of conditions3. A protein that contributes to purine ribonucleoside salvage by functioning in cytoplasm with guanine phosphoribosyltransferase activity, metal ion binding.4. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.5. For general function, the protein need meet that Purine salvage pathway enzyme that catalyzes the transfer of the ribosyl-5-phosphate group from 5-phospho-alpha-D-ribose 1- diphosphate (PRPP) to the N9 position of the 6-oxopurines hypoxanthine and guanine to form the corresponding ribonucleotides IMP (inosine 5'- monophosphate) and GMP (guanosine 5'-monophosphate), with the release of PPi.6. We require a protein that can specifically bind to the metabolites produced in the Purine metabolism, GMP from guanine: step 1/1 pathway. The designed protein sequence is ",
    "response#1": "MIDQKQVEAGCNAALKWCNEHFAGKQVIVLGILKGCIPFLGKLISQFTFDLQLDFVAVASYHGGSRQQEAPKIVLDMSHDPKGKDILLIEDIVDSGRSIKLVLDLLHTRKAKSVILVSFIEKLKPREADIKVDYSCFKNQDEFLVGFGLDYYEEEPIELKEEDIKRRVKELAKEIESSYSWEEPIVVVGLLKGAFIFLADLVRAFDRFVFVEFMQVSSYGKGMKSSGTIKIVKDLDMDIEGKEVLLVDDILDTGLTMKEIHDYLLMKKPKVLKTCVFLDKKERRKVDFNADFVGFEVPDKFLVGYGLSTKALDELKIKDQKQVEAGCNAALKWCNEHFAGKQVIVLGILKGCIPFLGKLISQFTFDLQLDFVAVASYHGGSRQQEAPKIVLDMSHDPKGKDILLIEDIVDSGRSIKLVLDLLHTRKAKSVILVSFIEKLKPREADIKVDYSCFKNQDEFLVGFGLDYYEEEPIEVLGKN",
    "response#2": "MIAKCAKKIAEDYRSFKLTTDNPLYLLCVLKGSFIFTADLARFLADEGVPVKVEFICASSYGTGVETSGQVRMLLDVRDSVENRHILIVEDIVDSAITLQYLMRFMLAKKPASLKTVVLLDKPSGRKVEVLVDYPVITIPHAFVIGYGMDSVEELSRGARALAWRLQGQGPGEDGAWRMVVAITRGGMAPAMIAARELDIRLVDTISVKSYSHQTQGAAEIIKTPDMDAVGDGTGVLIIDDLVDTGKTLEVVRALMPKAHVATVYAKPKGRPMVETFTHGQIDRARRKAPP",
    "response#3": "MEDQKQVEAGCNAALKWCNEHFAGKQVIVLGILKGCIPFLGKLISQFTFDLQLDFVAVASYHGGSRQQEAPKIVLDMSHDPKGKDILLIEDIVDSGRSIKLVLDLLHTRKAKSVILVSFIEKLKPREADIKVDYSCFKNQDEFLVGFGLDYYPLPAEPTMARALAWRLIDLGPFNGIVAITRGGLIPAAIIARELEARLVETVSVVTYGRGAAGETEIGAPQVLKPPVAAGDGEGFLLIDDLVDTGTTARVVRSLLPKAHFATVYAKPAGKQLIGDNYRDAAAETGQDLLLITVLKGAVIFVTDLARAIPLPTQFEFMAVSSYGSSTSSSGVVRILKDLDRDIQGRDVLIVEDVVDSGLTLSWLLRNLSTRHPRSLRVCTLLRKPDARGAHVDIAYVGFDIPNDFVVGYGLDE"
  },
  {
    "reference": "MKENDVVLRTVTKIVVFILLTFGFYVFFAGHNNPGGGFIGGLIFSSAFILMFLAFDVHEVLESLPIDFKKLMIVGAIISALTAIVPVFFGKSFLYQSEAYIHFPLLGELHVTTITLFELGILLTVVGVIVTIMLALSGGKS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have a specific function within the plasma membrane. The designed protein sequence is ",
    "response#1": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPT",
    "response#2": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPV",
    "response#3": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPS"
  },
  {
    "reference": "MKILTGTAIREADRFTIEHEPIESLALMERASEAIAQWICNHISQDVPLLFVVGKGNNGGDGLAVARMLHKVGYLCEAVVVFGRDQLSTECRTNLGRLPKGVRVSTRFDTLPDSGTVIVDALLGTGVRGEVTGLAADAIRQINASGCRVLSIDVPSGMVTEYGNLPGRLIVHADVTLTLEFPKLSLLLPEAGECAGVVTVLPIGLSEAYMEQVGTPYFYVDEELIRKMLLPRPRFGHKGCFGHALLVCGSEGMVGAAVLAAGAALRSGCGLVTVHLPREERVALQANHPAAMVSGDEQPCFSHLPQQPQRFTAIGVGPGLGQRLQTARALEALLQCGRPMVIDADALNLLAADPGLQELVPAGSILTPHPGELRRLVGEWEDDADRNERVRQLARRLSAYVVVKGAHTMVCTPEGICYFNPTGNAGMAKGGSGDVLTGLITGLLARGYPPLPAALVGVYLHGKAGDKATQYYGQEGVNAGDLADFIGETWAEWETGNTENPL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a fast turnover rate for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.2. The designed protein should contain one or more YjeF N-terminal domains that are essential for its function.3. The protein should have a high affinity for (6S)-NADPHX, K(+), AMP.4. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..5. A protein with ATP binding, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, metal ion binding, NADHX epimerase activity functions is required to enable nicotinamide nucleotide metabolic process.6. The protein must bind to Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MKMLERKTIEDFGISNLVLMERAGVSTVEVLWRELGNLSQYSFLVFCGPGNNGGDGLVIARELLNFTEAVKVVLISDLTTNESRKNLEIYKSFGGEIIKISKENILDVPKLLENSNVVIDAIFGTGLSRDVKGIYAEVIDYINLYSKFTVSVDIPSGVSADTANVLGTAIRSDLTVTFEYPKVGHFLFPGRALTGKLKVVKIGMPKILSLLTNEHFTVPNRLKESNKSSYGKVIIIGGSKEFIGAPLLSALGAIRAGTGRVYMAGPRDIVKSAVNYEPGIIPIAIENEYFSENDVNIVNNYIDSDTVVVIGPGLGRKNGVGLFVKKIVGSVNVPIIIDADAIYHISNLKDKIKDKDNLVITPHPGEFAKFLGMNISEVKYNYKLVKETSKAYNSIIALKDVTTIISNGESLFFNVTGNSSLSKGGSGDILSGLIAGFIAQNGDVLNGVLLGVYVFGLAAEMYEYEGHNLISEILDYIPKAIGIIGGNTGMNGALQLAGASALYSGCGKVSMIPLDQSFKADMSMPELMTKSLKNVPKNINIFSALAVGVGFDTTDDSQQILESIIDNLTQLAIFDADALNIIVTNQQIREKFINLENKIITPHPAEAARLLDCTTKEIQEDRFTAIRTLAKKYNATVVLKGAGSLICKDNKIYINTTGNQGMAVAGQGDVLSGIIGAFLAQGLNTLSASRLAVYVHGLAGDNVAKKLGIIGGNTGMNGALQLAGASALYSGCGKVSMIPLDQSFKADMSMPELMTKSLKNVPKNINIFSALAVGVGFDTTDDSQQILESIIDNLTQLAIFDADALNIIVTNQQIREKFINLENKIITPHPAEAARLLDCTTKEIQEDRFTAIRTLAKKYNATVVLKGAGSLICKDNKIYINTTGNQGMAVAGQGDVLSGIIGAFLAQGLNTLSASRLAVYVHGLAGDNVAKKL",
    "response#2": "MRDAENAAFARGIPAEALMNQAGAGIARAVQQFFSRPGYCLVFSGKGNNGGDALVAAAHLKRAGWHIDVHLSFPESECSELLRKKVTEFRDTPAVAAAASAANADGTPAPIIILDGLLGLGSRPPLRDPIIAACREINRLRREQNAYVFAVDQPTGLDGDSGKADSDCVVADFTVTIAAAKRGLVADDATNFVGRLEVVPLSDLPIETPAREAAVAGGSGDVLAGVELAEAHKGTFGHALIVGGSRGKGGAPALATVAALRMGAGLATAMIPGSALPAFHRRTLSGMSLPYGDEQVAYLNEAAALWGLRHLEGKTAVAIGPGLGQTPETAAFLREWLPALQVPCILDADALNLLAQDETLRARIRVPLLLTPHPKEAARLLGTSVEAVQARRLESALRLAQKWQALVLLKGAYPLVATPRGEAYFFRLAEPALATAGTGDVLTGMVVGLLAQGVEPLPALLLGVYVHGLAAKLATRGRLVLAGGVGRGGAARLAARAALRVGAGLVTLGCEAGAVAENAGRLDAVMLREVAGAEALAAVLQDARISALVAGPGLGLGAREAGLVAAALGGAHRPAVVLDADALTLVAQDATLFALLHDRCVLTPHEGEFARLFPDLAPRLGRGKVEVLRGAAKRAGCVVLLKGADTVIAAADGRCVVHAALRERAAPWLATAGSGDVLAGIICGLVARGWSGFAAAEAAAFLHVEAARELVLAGGVGRGGAARLAARAALRVGAGLVTLGCEAGAVAENAGRLDAVMLREVAGAEALAAVLQDARISALVAGPGLGLGAREAGLVAAALGGAHRPAVVLDADALTLVAQDATLFALLHDRCVLTPHEGEFARLFPDLAPRLGRGKVEVLRGAAKRAGCVVLLKGADTVIAAADGRCVVHAALRERAAPWLATAGSGDVLAGIICGLVARGWSGFAAAEAAAFLHVEAARE",
    "response#3": "MRRADEIMQIEYGYLGVLLMETAGRRAAQLVRRLYPKQERFVVAAGGGNNGGDGLVIARYLRRYGKEIFILLTQDPAKLSALSRIQYDILTRFGLSCHVLSPDSLRKLQAFCEAEPRPVLIDALIGVGLQQALRPNQMALLEFLRSLHLPVVAVDLPSGLSADTGALLNEPLRCEHTITFQVPKICHLVTPAALYCGQVHVVDIGIYPEVLPGDRRLLSRPADTHKGDFGEVLVVGGGPYTGAPALAAQAAMRAGADLVRVACPRAVADGVQGFEEGLILRPFDGDRLRIDHLDHVHELAAAHDAVVLGPGLGDHEATLAAVRDFLADFDGRAVVDADALGVVPDVDTDATLLCTPHGGELRGMGGPSADDWRDRLDAVADFAADLGHTLLVKGAYDVIADGERARVNRTGNPGMTVGGTGDVLAGAAGALLATQDPVDAGALAAYANGLAGDRVVDRQGYGLLASDLLPELPRTDLWAGARLPKLTPTMHKGDRGRVTVVGGSNGMTGAALHAARAALAAGAGLVKLVAGPETIDAARASLPDVLTVESALGPELEEEAAQALEWADALVIGPGLGREPARGQFLKAALACRPIPTVVDADALTLFQGPAPERPLVLTPHLGEFRAQFGDQLADAAANDRWAAAAKAAQKVKGTVLLKGVPTVIADLRGPIHVVASGNPGLATGGSGDLLAGFIGAFLARGSAGPEAAALGAHALGRAADHGARQWTARSLRPADVLAALPDIWRAAVEAAEVSAERLKKVEGHKYSHGHALVLAGGVGRGGAARLAARAALRVGAGLVTLGCEAGAVAENAGRLDAVMLREVAGAEALAAVLQDARISALVAGPGLGLGAREAGLVAAALGGAHRPAVVLDADALTLVAQDATLFALLHDRCVLTPHEGEFARLFPDLAPRLGRGKVEVLRGAAKRAGCVVLLKGADTVIAAADGRCVVHAALRERAAPWLATAGSGDVLAGIICGLVARGWSGFAAAEAAAFLHVEAAREFGPGLIAEDLPEMLPAVFRRLG"
  },
  {
    "reference": "MAEPTKISILGHESIIADFGLWRNYVAQDLISGCPSTTYVLITDTNIGSIYTPGFEKAFEDAAASVSPSPRLLVYNVPPGEVSKSRQTKADIEDWMLSQSPPCGRDTVVIALGGGVIGDLTGFVAATYMRGVRFVQVPTTLLAMVDSSIGGKTAIDTPLGKNLIGAIWQPTRIYIDLEFLETLPVREFINGMAEVIKTAAISSEEEFTALEDHAETILEAVRRDLKPGQHRFKGIEEILKARILASARHKAFVVSADEREGGLRNLLNWGHSIGHAIEAILTPQILHGECVAIGMVKEAELARHLGILKGVAVSRIVKCLTAYGLPTSLKDARIRKLTAGKHCSVDQLLFNMALDKKNDGPKKKVVLLSAIGRTYEPRASVVANEDISVVLAPSIEVHPGVAQSLNVVCAPPGSKSISNRALVLAALGSGTCRVKNLLHSDDTEVMLNALERLGAATFSWEEEGEVLVVNGKGGNLQAHPSPLYLGNAGTASRFLTTVATLANPSNTDSSVLTGNNRMKQRPIGDLVDALRANGASVEYMERTGSLPLKIAASGGFAGGNINLAAKVSSQYVSSLLMCAPYAKEPVTLKLVGGKPISQPYIDMTTAMMRSFGIDVQKSTTEEHTYHIPQGRYVNPAEYVVESDASSATYPLAIAAVTGTTCTVPNIGSASLQGDARFAVEVLQPMGCTVEQTETSTTVTGPSDGILRPLPNVDMEPMTDAFLGASVLAAIARGKDGNHTTRIYGIANQRVKECNRIKAMKDELAKFGV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity.2. The design of the protein must focus on the precise arrangement of amino acids in the For EPSP synthase activity, Proton acceptor; for 3-dehydroquinate dehydratase activity to enable effective catalysis of NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH.3. The NAD(+), 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate, ATP binding site should be located in a region of the protein that is accessible to the ligand.4. A protein should be able to manipulate the activity of enzymes in the chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7, Metabolic intermediate biosynthesis pathway, either by activating or inhibiting their function.5. The protein must exhibit the following characteristics: The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis..6. A protein that can perform ATP binding, 3-phosphoshikimate 1-carboxyvinyltransferase activity, 3-dehydroquinate synthase activity, shikimate 3-dehydrogenase (NADP+) activity, metal ion binding in cytoplasm to support amino acid biosynthetic process, phosphorylation, aromatic amino acid family biosynthetic process, chorismate biosynthetic process. The designed protein sequence is ",
    "response#1": "YVVSPGEANKTRDTKAKIEDFLLSKGCTRDTVILAIGGGVIGDMIGFVAATFMRGVRVVQVPTTLLSMVDSSIGGKTAVDTPLGKNFIGSFWQPQYVFVDLTFLSTLPEREFEAAAEVFYNPQMDLSAVSCTPPGSKSISNRALVLAALGNGECRIRNLLHSVDTEVMIEALKIMKCASFSSGEDSKGKYLLVTGNGGNLQASEKDLYIENAGTAARFLATVALLAKPNLQSYSILTGNKRMNEGRPIKDLVDALRANGSSIECTGKDGHLPLKIEAAEGMEGGDIVLDASFSSQYVSSILMAAPYAKKPVTLRLTGGPPISQLYIDMTTAMMRKFGVDVTKSTIEEHAYHVPQATYRNPPDYEVESDASSATYPLALAAINGITCIVPNVGYGSLQGDAQFAVKVLKQMGCSVDQTESSTTVTGPPKGTLKPIPSIDMETMTDAFLTASILAAVAQGSTGKSTTKITGGMAEVIKTACIWNADEFIRLEVNADPFLEIVNNCQSITHKNKITGNIEKICDCKIEDILDHVYRLVLESIKVKAEVVSSDERESSLRNLLNFGHTVGHAYEAILTPQALHGECVSIGIIKEAELSRYLGILSTTQVSRLYKILVAYGLPVSPNEQWFKELTLQKKTPLNVLLDKMSIDKGMAEVVKTAAIWNADEFAALESRSSEIFAAIQTPSQFNAGRTLSTRSEAQALLLQVIVGSISVKAHIVTIDERETGLRNLVNFGHTIGHAIEAVVTPQILHGECVSIGMILEAEIARLLGVLGQVAVGRLTRCLKAYNLPVSVHDPRIASLPTSKLLTIDRLLDIMKVDKK",
    "response#2": "MTYVVSPGEANKTRDTKAKIEDFLLSKGCTRDTVILAIGGGVIGDMIGFVAATFMRGVRVVQVPTTLLSMVDSSIGGKTAVDTPLGKNFIGSFWQPQYVFVDLTFLSTLPEREFSAVDVETVYLGNAGTAARFLTGAAIKTKPEANDHRLSKVREKLKDIVLGSVQVKAHVVSADEREGGLRNLLNFGHSIGHGIEGLLTPDILHGECVAIGMVLEAELALHLGVLSREAVARLRNCLAKYELPTSLQDAVVRERSGHKHCSMDQILSVMAVDKKGMAELIKTAAISDENDFCKLENGVEAIRHAVLGNDSNVPNQGATLDTRTEAQAMLMDVILASVRFKAYVVTSDEREGGLRGLLNFGHSIGHGIEAIVSPLMLHGECVSIGCMKEAELARNLGHLNQVAVGRLARCFQSYGLPICMEEKKVKDRIGSIYCHVDDIMEIMKVDKKKR",
    "response#3": "MTYAVKPGEANKTRSTKADIEDYLLSKGCTRDTVIVAIGGGIIGDMVGFVAATFMRGVRVIQVPTSLLAMVDSSIGGKTAVDTPLGKNFIGAFWQPQFVLVDVKWLETLPRREFESLCADYFGHPTHELHIYLQNAGTAARFMTGMAEVIKTACIWNADEFIRLEVNADPFLEIVNNCQSITHKNKITGNIEKICDCKIEDILDHVYRLVLESIKVKAEVVSSDERESSLRNLLNFGHTVGHAYEAILTPQALHGECVSIGIIKEAELSRYLGILSTTQVSRLYKILVAYGLPVSPNEQWFKELTLQKKTPLNVLLDKMSIDKAAEHLKDDYKLVIPKDLKNEVIPPGSKSVSNRALVLAALGNGPCRIKNLLHSDDTEFMLTAIEKLGGATYAWEDAGEVLLVQGKGGDLRASPTEIYIGNAGTASRFLTTVVSLCKPSTSKETVLTGNTRMKVRPIGPLVDSLRTNGVDIQYLEKPNSLPLKVEASGGFEGGDIELAATVSSQYVSSILMCAPYAKKPVTLRLVGGKPISQPYIDMTTAMMASFGIKVVRSDTEEHTYHIPKGVYQNPKEYVVESDASSATYPLAVAAISGSTCTIPNIGSASLQGDARFAIEVLRPMGCTVIQTDRSTTVTGPAFGRLKAIEEVDMEPMTDAFLTASVLAAVAKGTTRIRGIANQRVKECNRILAMKDQLAKFGVVCRI"
  },
  {
    "reference": "MLHTIDEALDDLKKGKLIIVVDDEDRENEGDFITAAANVTPEIINFMSKYGRGLICAPITESRADALQLNLMVEDNTVLHQTPFTVSVDLIGQGCTTGISAHDRAKTVQALIDPATKPSDLGRPGHIFPLRAKPGGVLRRTGHTEATVDLARLAGFEPAGVLVEIMNDDGTMARLPELMEISEKYDLKIISIKDLIAYRLEKESLIEEEVRVDMPTKYGHFELIAFRQKNTGEQHLALIKGDWKEGDAVLTRVHSSCFTGDILHSLRCDCGEQLQAAMQMVEQEGKGCVLYMNQEGRGIGLINKLKAYKLQEEGKDTVEANLALGFKMDERDYGVGAQILRHLDITKIKLMSNNPRKRAGLLGYGIEIVDIVPIEIKPNPYNEKYLTTKRDKLGHDILK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The presence of GTP cyclohydrolase II domains is critical for the interaction of the designed protein with its intended targets.2. A protein should be able to manipulate the activity of enzymes in the 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4, riboflavin biosynthesis pathway, either by activating or inhibiting their function.3. The protein's Nucleophile; for GTP cyclohydrolase activity must be carefully engineered to ensure that the amino acids involved in GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate are properly positioned for optimal catalysis.4. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+), Zn(2+) binding.5. The presence of Zn(2+) is crucial for the protein to function optimally under specific conditions.6. The protein design must be able to perform GTP cyclohydrolase II activity, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, GTP binding, zinc ion binding, manganese ion binding in order to contribute to riboflavin biosynthetic process. The designed protein sequence is ",
    "response#1": "RMASASLPSEFGNFVMIGYESALSSDHHVAIVKGDIKNKKNVLVRVHSECMTGDIFGSYRCDCGVQLHHALDKIEEEGLGVVVYLRQEGRGIGLINKLKAYELQECGRDTVEANEDLGFSPDLRDYGIGAQILADLGLSSIRLMTNNPRKIVGLEGYGLEITEVVPEEENIYFNAFLPTKFGDFKIILYHSETDSKDHVALVKGEISPDKPTLVRVHSECLTGDVFGSLRCDCNDQLQSALKIINEAGSGVVLYMRQEGRGIGLSGKIRAYRLQDEGLDTVEANEKLGFRADLRDYGIGAQILLDLGVRKIRLLTNNPKKIVGLEGYGLEIVERIERVSEASMPIKYGEFKIIGYVNKLNGEHHVALVKGEINNEMPVLVRVHSECLTGDAFGSLRCDCGEQLQSALSAIEEEGKGILLYMRQEGRGIGLINKIKAYALQDKGMDTVEANLALGFPEDMRDYGIGAQILADLGAKKIRLMTNNPKKLSGLSGYGIKVVERVPKKEIYEVVRVHMPTKYGDFQLVAFKEKHNAAEHLALIKGSWNPGDAVLTRVHSSCVTGDILGSLRCDCGDQLHAAMRMVEKEGTGMVLYMNQEGRGIGLINKLRAYKLQEEGLDTVEANLKLGFKADQRDYGVGAQILRHLGATKLKLMSNNPTKRAGLLGYGIEIVEVVPEEENIYF",
    "response#2": "VASALLPTAFGDFVAVGFRSEIDGIEHIALVRGEIGAGDDVLVRVHSECLTGDVFGSLRCDCGPQLQAALRRIAEEGRGVVLYIRGHEGRGIGLLHKLQAYELQDNGRDTVDANLELGLPADARDYGTGAQILADLGIRSMRLLTNNPTKRAGLEGYGLSIVEPVPADEVPLGDDAERVAVTKLPTTHGAFTAVGYRGTLDGTEHLALVAGGLDAEGRLPDGEDVLVRVHSECLTGDVFGSLRCDCGPQLQASLAQVAAAGRGVVLYLRGHEGRGIGLGHKLRAYELQELGHDTVDANLELGLPADARDYSIGAQMLCDLGVRSLTLLTNNPQKLNALTEHGLKVKERRGWPLER",
    "response#3": "RMASASLPSEFGNFVMIGYESALSSDHHVAIVKGDIKNKKNVLVRVHSECMTGDIFGSYRCDCGVQLHHALDKIEEEGLGVVVYLRQEGRGIGLINKLKAYELQECGRDTVEANEDLGFSPDLRDYGIGAQILADLGLSSIRLMTNNPRKIVGLEGYGLEITEVVPQEIDEVVRVHMPTKYGDFQLVAFKEKHNAAEHLALIKGSWNPGDAVLTRVHSSCVTGDILGSLRCDCGDQLHAAMRMVEKEGTGMVLYMNQEGRGIGLINKLRAYKLQEEGLDTVEANLKLGFKADQRDYGVGAQILRHLGATKLKLMSNNPTKRAGLLGYGIEIVEVVPEQIDLGETPVFERVSVNLPTLYGKFVLHLYRSFVNEYLHLALCLGEIGSLDNSPAPIQTEPVLIRVHDECLTGDIFGSSRCDCGEQLHNALKMIQQEGKGILLYMRQEGRGIGLENKLHAYALQENGFDTVEANKKLGFVADKRDYGIGAQILRDLGVSKMRLLSNNPKKFTALAGYGLEIVERIERVTQAHMPTKYGNFKMVGYVNKVNGEHHIALVKGDITDGEPVLMRVHSECLTGDAFGSLRCDCGDQFAAAMKKINEEGRGLLLYMRQEGRGIGLINKIKAYALQDTGMDTVEANLALGFSEDMRDYGIGAEIIRDLGIKKIKLMTNNPKKINGLEGYGLEIVERVERVTQAHMPTKYGNFKMVGYVNKVNGEHHIALVKGDITDGEPVLMRVHSECLTGDAFGSLRCDCGDQFAAAMKKINEEGRGLLLYMRQEGRGIGLINKIKAYALQDTGMDTVEANLALGFSEDMRDYGIGAEIIRDLGIKKIKLMTNNPKKINGLEGYGLEIVERV"
  },
  {
    "reference": "MSKTIADLLVETLSDAGVQRIWGVTGDSLNGINDSLRRFKRIEWVPVRHEETAAFAAGAEAAVTGKLAVCAGSCGPGNMHLINGLYDCWRNRVPVLAIAAHIPSTEIGLGYFQETHPQALFRECADFVELVSNPAQMPEILYRAMNTAIGKQGVAVLVLPGDVALSEAPAASPRHWVAEQPQRVAPTSTEIDRLVGLLNQAKAPAILAGAGCAGAHTELVSVAKALKAPIVHALRGKEYVEYDNPYDVGMTGLIGFSSGYHTLMSADAVLMLGTSFPYRAFYPKEATMIQVDRDPTALGRHTTLSLGIAADVRETLSALLPKLAERSDTTFLDHALAHYHKARSGLDELAQPAGAGKPVHPQHLMRLVSELAAEDAVFTADVGTPTVWAARYLKMNGRRRLLGSFNHGSMANAMLQALGAQAAQPNRQVISLSGDGGFTMMMGDMLTAVQQKLPIKIILFNNSSLGFVAMEMKAGGYLDTATDLHNPDFAAMAKVAGFTSFRVEDSTNLKETLTQALAHPGPVLVDVRTERNELIMPPKVQIGHAKGFSLYMLKAVLNGRGDEVVELARTNLR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be involved in pyruvate catabolic process by localizing to plasma membrane and exhibiting ubiquinone binding, magnesium ion binding, flavin adenine dinucleotide binding, thiamine pyrophosphate binding, pyruvate dehydrogenase (quinone) activity.2. The protein have enhanced a ubiquinone + H2O + pyruvate = a ubiquinol + acetate + CO2.3. For general function, the protein need meet that It channels electrons from the cytoplasm to the respiratory chain at the cell membrane via ubiquinone.4. The presence of Thiamine pyrophosphate enzyme TPP-binding, Thiamine pyrophosphate enzyme central, Thiamine pyrophosphate enzyme N-terminal TPP-binding domains is critical for the interaction of the designed protein with its intended targets.5. The protein should be able to bind FAD, thiamine diphosphate, Mg(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "TIAEDIVEIIKDAGVTRIYGLPGDSLNGFTDALRRDGTIAWQHVRHEEAAAFAAAADAALTGGLAVCAGSCGPGNTHLINGLYDANRSRVPVLAIAAQIPGPEIGSTYFQETHPQDLFREASVYTELVSYPEQLPHVLEIAMRTAVERRGVAVVVIPGEVFLRESASGAALAGDAALARMLNDCSRVTLLCGGGCAGAHDEVVALAAALKAPVVHALRGKEHVEYDNPYDVGMTGLIGFSSGYHAMKECETLLMLGTDFPYRQFYPTEARIAQVDLRGEVIGRRCRVDVGVVGDVKLTLLHALTGQPLARVAEVTDALAALLNAGKRVTLLCGRGCAGAHDELMALAGALKSPIVHALGGKEYIEYDNPFDVGMTGLIGFSSGYYAMEDCDTLLMLGTDFPYRQFYPEDATIAQVDLRPANIGRRCRVDLGVVGDVKATLQTLRARGRVNDLVGAIHPQYVVRVLDEIAAPDAVFSCDVGTPTVWAARYLTMNGQRRLLGSFNHGSMANALPQAIGAQVSHPRRQVISLSGDGGLAMLMGDLLSLRQLQAPVKVIVFKNNALAFVELEMKAAGFLDFATDLHNPDFAKMAEAAGLLGMTAEAPEQVRPMITQALRHDGPALVEVLVHRQELSMPPAITAQDVGSPVVWASRYLTMNGRRRLIGSFSHGTMANAVSQGIGAQASHPSRQVIALAGDGGLTMMLGELLTLVQNQLPVKVVVFNNSSLNFVELEMKAAGLVNSGTELVNPNFADMARAMGLHGVRVERPDDLPSALADAFAHDGPALID",
    "response#2": "TIAENMVDTLKASGVSRIYGLAGDSLNGLTDALRGELDWVHVRHEEAAALAASAEAALTGELAVCAASCGPGNLHLINGLFDANRSRVPVLAIASHIPSSEIGSNYFQETHPQELFRECSVYCEMVGSPEQMPRILEIAMRTAIERRGVAVVVIPGDILQKMPTDALAALLNAGKRVTLLCGRGCAGAHDELMALAGALKSPIVHALGGKEYIEYDNPFDVGMTGLIGFSSGYYAMEDCDTLLMLGTDFPYRQFYPEDATIAQVDLRPANIGRRCRVDLGVVGDVKATLQTVTMLTPRQAEAARLLNDADKVTILAGAGVAGAHSEVLALADALGAPIVHAMRGKEHIEYDNPFDVGMTGLLGFASGYSAMLSCDTLLMLGTDFPYPQFFPENAKVIQVDVRGEQLGRRTPLDLGLVGTVRDTAQAVLGRPLDPPALLSAAEDVGSPTVWAARYLRMNGRRRLIGSFNHGSMANALPHAIGAQSAFPERQVVALAGDGGLAMLLGELLTLRQLELPVKLVVFNNSSLNFVELEMKAAGFVNYGTGLENPNFADLANAVGLHGQRVEHPDDLERALTAAFAHDGPALVDDVGSPVVYAARYLSCRGDRRVIGSFVHGSMANAVPHAIGAQSAYPDRQVVTLSGDGGLAMLMGELLTVRQHDLPIKVVVLNNSSLNFVELEMKAAGLPDFGTGLDNPDFSKVAEAVGIRGWRVERSEDLEGALREAFAHDGPALVDLTAS",
    "response#3": "TIAEDIVEIIKDAGVTRIYGLPGDSLNGFTDALRRDGTIAWQHVRHEEAAAFAAAADAALTGGLAVCAGSCGPGNTHLINGLYDANRSRVPVLAIAAQIPGPEIGSTYFQETHPQDLFREASVYTELVSYPEQLPHVLEIAMRTAVERRGVAVVVIPGEVFLRRDATEILNSAAKVTILGGAGCNGAHKELIATADALKAPIVHALRGKEYIEYDNPFDVGMTGLLGFSSGYHAMEDCDVLLMLGTDFPYPQFFPSHAKIIQVDLRGNQIGRRTKVDLGLVGSIKDTLSALARLVTGTPAPPDFAEVARLAETLNCAKNITLLCGSGCAGAHAEVVKLAETLKAPVVHAMRGKEHIEYDNPYDVGMTGLIGFSSGFHAMMNADTLVLLGTQFPYRAFYPADAKIIQIDINPGSLGSHSHVDMALVGDIKTTLHALLPRLPTPGDTELLDRVRERYRAHGRELDRLAVGPRGERMHVQKLLTAASRGEGGRYLSFVLDDQ"
  },
  {
    "reference": "MFDDIETAVRRIADGGVIIVVDDEDRENEGDFVAGAEKATPEMVNFMTKFGRGMVCAPAEPERIEVLGLDLMVDKNTALHGTPFTVTVDYTEGTTTGISAHDRAATLRALADPRVGSEKFARPGHIFPLKASSGGVLRRAGHTEASVDLCKMAGLYPVGVICEIMADDGSMARVPQLAKIAREHEIPIITVKDLILHRSKTERLVRKVTSAEFPTKFGDFDITLYESLLDGKQHIALVKGDVAGKQNVLVRVHDECLTGDVFGSRRCDCGEQLADAMTMIESEGEGVLLYMRQEGRGIGIGKKLQAYHIQDLGFDTVDANCRLGLPPDLRDYGIGAQILVDIGLSSIRLLTNNPRKIVGLEGYGLSIVERVPISVSPNSHNIGYLKTKQKRMGHLLDFEEGTDAPNRNISQRSEDEHC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein with manganese ion binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, magnesium ion binding functions is required to enable riboflavin biosynthetic process.2. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..3. The Proton acceptor; for GTP cyclohydrolase activity active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.4. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity.5. The Zn(2+), Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.6. The protein should be optimized for activity in Cofactor biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "RLAETVLPTAIAGEFKAIVYESEVDPNHHLALVKGEIDSEQPILVRVHSQCLTGDVFGSLRCDCGSQLHTAMKMIEKEGKGVLVYMRQEGRGIGFANKMKAYALQDAGHDTVEANLALGFKPDLRNYGIGAQILRDLGVRKMRLMTNNPKKIVGLEGYGLEVVERIAHVQSRAAGELRVATAHLPTQWGHFIAHAYEADIEANPYIALVMGDVTSEEPVLVRIHSGCLTGDALRSLRCDCGDQLDLAMRRIAEEGRGVLLYIQQEGRGIGLVNKLKAYVLQDHGADTVEANELLGFPADIRDYSLGAQVLEDLGLRRIRLMTNNPAKFAALEGYDLEIVERVRVATAHLPTQWGHFIAHAYEADIEANPYIALVMGDVTSEEPVLVRIHSGCLTGDALRSLRCDCGDQLDLAMRRIAEEGRGVLLYIQQEGRGIGLVNKLKAYVLQDHGADTVEANELLGFPADIRDYSLGAQVLEDLGLRRIRLMTNNPAKFAALEGYDLEIVERVREVEARVPTEHAGEFKAVVYSNSVDHLDHLALVKGDISKAERVLVRVHSECLTGDVFGSARCDCGLQLNAAMKMIDQEGCGIVLYMRQEGRGIGLVNKLKAYCLQDDEGVDTVEANHRLGFKSDLRDYGIGAQILRDLGVKKMAILTNNPKKIVGLEGYGIEVVERLAEQTRPLPRVSEAKLPTPMGTWQLIGYRTRDHSEEHVALVMGNVGGGEPTLVRVHSQCLTGDVFSSQRCDCGEQLEIAMRHVAEERRGVIVYMAQEGRGIGLHNKIAAYALQDQGMDTVEANLALGFAADRRDYGIGMQILRDLGVHRIRLMTNNPAKRHGLSGYGLEIVERV",
    "response#2": "ERVVSTRLPTAHGEFTAHGFRSGIDGSEHIALVHGDITTGTPLVRVHSECLTGDVFGSLRCDCGPQLDAAQAAVVAAGSGVIVYVRGHEGRGIGLVDKLRAYAAQDAGADTVDANSDLGLPVDARDYTHAAQMLRDLGVESVRLLSNNPAKVSALTSLGVEVVERRVATAHLPTQWGHFIAHAYEADIEANPYIALVMGDVTSEEPVLVRIHSGCLTGDALRSLRCDCGDQLDLAMRRIAEEGRGVLLYIQQEGRGIGLVNKLKAYVLQDHGADTVEANELLGFPADIRDYSLGAQVLEDLGLRRIRLMTNNPAKFAALEGYDLEIVERVLGA",
    "response#3": "RLAETVLPTAIAGEFKAIVYESEVDPNHHLALVKGEIDSEQPILVRVHSQCLTGDVFGSLRCDCGSQLHTAMKMIEKEGKGVLVYMRQEGRGIGFANKMKAYALQDAGHDTVEANLALGFKPDLRNYGIGAQILRDLGVRKMRLMTNNPKKIVGLEGYGLEVVERIREVEARVPTEHAGEFKAVVYSNSVDHLDHLALVKGDISKAERVLVRVHSECLTGDVFGSARCDCGLQLNAAMKMIDQEGCGIVLYMRQEGRGIGLVNKLKAYCLQDDEGVDTVEANHRLGFKSDLRDYGIGAQILRDLGVKKMAILTNNPKKIVGLEGYGIEVVERLRVATAHLPTQWGHFIAHAYEADIEANPYIALVMGDVTSEEPVLVRIHSGCLTGDALRSLRCDCGDQLDLAMRRIAEEGRGVLLYIQQEGRGIGLVNKLKAYVLQDHGADTVEANELLGFPADIRDYSLGAQVLEDLGLRRIRLMTNNPAKFAALEGYDLEIVERVRVSEAKLPTPMGTWQLIGYRTRDHSEEHVALVMGNVGGGEPTLVRVHSQCLTGDVFSSQRCDCGEQLEIAMRHVAEERRGVIVYMAQEGRGIGLHNKIAAYALQDQGMDTVEANLALGFAADRRDYGIGMQILRDLGVHRIRLMTNNPAKRHGLSGYGLEIVERVGG"
  },
  {
    "reference": "MALLNDVTSVAECNRQTTMTDEHKSNINSNSSHSSNNNNNGSSSNNDNNSNDDAASSSNSKNNNTSNESSHSNNNTSSIIAEAAAKFLLKNGLNGSSSTSYPPLPPPLPANLSRTTTPTTTTTPSSSSSTASNGFLPHAKTPKSSSIMAASAAVAASVVGATASKPTIDVLGGVLDYSSLGGAATGSLPTTAVVAAAAGTAKIGKGSNSGGSFDMGRTPISTHGNNSWGGYGGRLQFFKDGKFILELARSKDGDKSGWVSVTRKTFRPPSAATSATVTPTSAVTTAYPKNENSTSLSFSDDNSSIQSSPWQRDQPWKQSRPRRGISKELSLFFHRPRNSTLGRAALRTAARKRRRPHEPLTTSEDQQPIFATAIKAENGDDTLKAEAAEAVEIENVAVADTTTNEIKIEKPDTIKGEDDAERLEKEPKKAVSDDSESKEASPGQQVEPQPKDETVDVEMKMNTSEDEEPMTELPRITNAVNGDLNGDLKASIGKPKSKPKPKAKLSSIIQKLIDSVPARLEQMSKTSAVIASTTTSSDRIGGGLSHALTHKVSPPSSATAAGRLVEYHTQHVSPRKRILREFEKVSLEDNGCVNNGSGGASSGGAGGKRSRAKGTSTSSPAGKASPMNLAPPQGKPSPSPGSSSSSTSPATLSTQPTRLNSSYSIHSLLGGSSGSGSSSFSSSGKKCGDHPAAIISNVHHPQHSMYQPSSSSYPRALLTSPKSPDVSGSNGGGGKSPSHTGTKKRSPPYSAGSPVDYGHSFYRDPYAG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have DNA binding, transcription corepressor activity within RNA polymerase II transcription repressor complex to regulate imaginal disc-derived wing vein morphogenesis, negative regulation of transcription by RNA polymerase II, wing disc dorsal/ventral pattern formation, somatic stem cell population maintenance, negative regulation of Notch signaling pathway.2. strand-strand-strand-strand is the desired arrangement for the engineered protein.3. For general function, the protein need meet that Is a potent antagonist of neurogenic gene activity during sensory organ development; The expression of distinct cell fates by the trichogen (shaft) / tormogen (socket) sister cell pair depends on the level of H activity; A certain threshold level of H activity is required, below which both sister cells adopt the tormogen fate.4. Prioritize Polar residues, Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "QPSPAHTLFTLTLEQHWVSKEGLIQHRLSTASFSDLCASERWGEPPPGHPHDAGLQMLEQVVNTLTDPTIMYGVNGNIPYGQTTLTTLLKDSFGGRAQTLVILCVSPLEEHLVETLGNLQFAFKVL",
    "response#2": "QPSPAHTLFTLTLEQHWVSKEGLIQHRLSTASFSDLCASERWGEPPPGHPHDAGLQMLEQVVNTLTDPTIMYGVNGNIPYGQTTLTTLLKDSFGGRAQTLVILCVSPLEEHLVETLGNLQFAFKVQ",
    "response#3": "LPLHCVVESVHSLHASLTIDTRQPWKRRPNIETDSYVIIAAXTPWSEIVQTALQRLGYSQEVANTARGSLIIKHWKPIPLEQISDNPAVPVSDIVGELTSVITLRII"
  },
  {
    "reference": "MILLKIIHIIFVMIFLSIFFPKSLYASTSLIRKDHIIQKNLENFDDQSAYALGVSLGNYINHSFREQKRLGVILDKNSLLSGIRDSLSGKTILSDQEISMELIKLEKKLKYFEDIVLKKEAHNNKIQGDLYIKKMLKKKDARHTSSGLVFFIKKKGSGKFLHDSDVITVHYKGSLINGNEFDNSYKRGQPLSFSLDSVIPGWIEGLKYIKKGGLIKLVIPPKLAYGETGVPGIPGNSTLIFEIELIDIQSK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should be able to enhance the efficiency of protein folding. The designed protein sequence is ",
    "response#1": "TESSRYVKKTDAELKRVLTPLQYEVTQHAATERPYDNAYNDEFRPGIYVDITTGEPLFLSTDKFESGCGWPAFSKPIDPHFLEEKQDSSHDMQRTEVRSAKGQAHLGHVFKDGPKDRGGLRYCINSASLRFIPR",
    "response#2": "TESSRYVKKTDAELKRVLTPLQYEVTQHAATERPYDNAYNDEFRPGIYVDITTGEPLFLSTDKFESGCGWPAFSKPIDPHFLEEKQDSSHDMQRTEVRSAKGQAHLGHVFKDGPKDRGGLRYCINSASLRFIPG",
    "response#3": "VHELRVSLEEIYHGSTKRMKITRRRLNPDGRTVRTEDKILHIVIKRGWKEGTKITFPKEGDATPDNIPADIVFVLKDKPHAHFRRDGTNVLYSALISLKEALCGCTVNIPTIDGRVIPLPCNDVIKPGTVKRLRGEGLPFPKVPTQRGDLIVEFKVRFR"
  },
  {
    "reference": "MSSLAAPQTGLLRQYLVLTKPRVTQLAVFCAVIGMFLAAPGMPDLSHVVFGTLGIWLLAAAAFAINCLIEQEVDARMLRTARRATARGTISDIQVLSLSGLLGGAGMLVLYHLVNPLTMWLTFATFVGYAIIYTVILKPRTPQNIVIGGLSGAMPPALGWAAVADSVPAEAWVLVLIIFIWTPPHFWALALYRNNDYIKAGLPMLPVTHGQQFTRLHILLYSFALLATTLLPYAIRMSGALYLASALALGGMFVWYAWRLYREYSDALARRLFRFSILYLALLFGALLIDHWVGLLR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that can perform protoheme IX farnesyltransferase activity in plasma membrane to support heme O biosynthetic process.2. We require a protein that can specifically bind to the metabolites produced in the heme O from protoheme: step 1/1, Porphyrin-containing compound metabolism, heme O biosynthesis pathway.3. The protein must exhibit the following characteristics: Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.. The designed protein sequence is ",
    "response#1": "LNATC",
    "response#2": "GYRMI",
    "response#3": "SWSP"
  },
  {
    "reference": "MLSVGQSLPGVPLRADGLRQTPKPTHNLNGEFTFDTFVEGKSNELAKAASLQVASNSGIAYNPLLLYGGVGLGKTHLMQAVGNEILKVNPDAKVVYLHSQKFVQDMVKALQQGTMQDFMQYYRSVDALLIDDIQFFAKKLRSQEEFFHVFNALLERGHQMVLTCDRYPREIDGLEDRLKSRFVWGLTVEVEPPELETRVAILMKKAEAERVDLDPAVAFFIAERIRSNVRELEGALRRVIVKRALRDLFAIQVAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKAKDNRLEITGTDLEVELVAECDADVQQPGADRAIVRSGRSRFTLATLPAEDFPKVPGGEADVALQLKQADVRGLIDEVGFAMAQQDVRYFLNGMLFEVTGEHIRAVATDGHRLAMSTKDCSNPGVAQRVQAIVPRKGVLELARLLDGEGETIELQLGGNHLRAKTGEYTLTTKLVDGQFPDYEKVVPTDGSRALVGDRETLRHAFQRAAILSNEKYRGVRLIVAPELLTIQANNPEQEEAEEIVAVEFNGDELEIGFNVSYLLDVLGVLQTDSVRLSVSDANSSALIEGIGRSFRTHRIQPVVRQGEIALVVTGEVVSDEGVITKLGTQRSTSGTTELRLGGEKTAKASALAQILPIQTLLPDAADLVLGSPGGRRAFLDWGLFHVEQTFLESSGYYRRVLSQRNAWLKQLATSQSTIAEDPWFQQYRDYAISISTSRAKYVAGLSLVFRGLMKELNASLDVELSYDWGGLVSAEDADKKV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The ideal protein would be one that plays a critical role in DNA repair through metal ion binding within chromosome. The designed protein sequence is ",
    "response#1": "SGAFIVNGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGHLLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEC",
    "response#2": "SGAFIVNGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGHLLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEC",
    "response#3": "PYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGHLLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTC"
  },
  {
    "reference": "MTAATGAAPSRGAADPARTPRRKGLLSRVPQGFATFFGALALLCALLDLIAPLRVLLHPVVRVLDLLLVPVSVNLAYAVFLVLLAGATAARKKVAWWLVVGYLGLVILGDVLGLAAGEYAESAPSLVLCGLALVLLITARGEFYAASRRGSWWRALAVLVAGLAVAVLAGWGLVALFPGTLPSGLHLAWSANRVMGGLISTRDFDGHPPRPVTFLLGLFGALALLNAAATLFRSQRLEAALHGDEEPRIRALLKAYGEDDSLGYFATRRDKAVVFSPSGKAAVTYRVETGVCLASGDPVGDREAWPHAIAAWLDAARRYAWAPAVMGASEEGARAYARAGLGALQLGDEAIVHVAGFDLDGRDMRVTRQAVRRVRRTGATCRIRRHATLTDAEMEEIVDKADAWRDTETERGFSMALDRLGDPADGDCLLVEALDAEGRLLALLSFVPWGRDGASLDLMRRDRAAPNGVMEYMVAELCAAAPKLGVRRISLNFAVFRSVFEEGARIGAGPVLRLWRGLLLFFSRWWQLEALYRSNAKYHPEWYPRFICYGETASLARIGLASGIAEGFVAVPSLRKLWGKGHPAHPATGPRPATTEGLPPLTALGLDEEARAEAGGPLAGLPEQVRVRHARLDRLRAAGTDPYPVGTKARTRTLAEVREGEQLTVAGRILLVRDFGGIVFVVLRDWSGDHQLALTRDRTGPAALKRFTADTDIGDHITATGRAGRSDRGEPTVFVTSWRLLGKCLRPLPDKRRGLTDPETKVRLRYLD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein have enhanced ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-   tRNA(Lys).2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.4. The designed protein should contain one or more Aminoacyl-transfer RNA synthetases class-II family profile domains that are essential for its function.5. The protein must exhibit the following characteristics: Catalyzes the production of L-lysyl-tRNA(Lys)transfer and the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge; In fact, the modification of anionic phosphatidylglycerol with positively charged L-lysine results in repulsion of the peptides.; LPG synthesis contributes to the resistance to cationic antimicrobial peptides (CAMPs) and likely protects M.tuberculosis against the CAMPs produced by competiting microorganisms (bacteriocins). The designed protein sequence is ",
    "response#1": "REQVARWIGRVYLFAAIWSLISVPLRHNIVVEIVADLLGLVNVPSEPSLFIVCLLFVLAGAVRRRLRAGFTMVVILMGISVLVSLAVMLSAIFRQEWLQDTQLRGFERDYYSFITSVPVTAVFFAIGLAVLVVVARSRSAFPARLAEGSRLAAVLVLVAGLATSFAITFALAATAPKELVGTREIGRFALRSTFGIASDSTSPGMHGHVGYAWIYGLAGIMSTIALLLASLFLLYRGLKSSLPAAAGWTVGVIATLSLIASVSPVIRWVIREPREFINDYIFNFPDTSFAWSFVLALLAGALTARKRIAWWVLVLNLLIAAGLNVAGLAADHVSPARAFGETLGLGLHLAALVVLGLAYREFWAKVRQGALFKAAGVLVAGWAVGIALSWTLIEMFPRSLAVDDRLFYVVNRVVGFALVASASFSGRPPIFLNALFGLFSAMALIAAAIVLYWWVPAAAGWTVGVIATLSLIASVSPAIRWLIKEPREFVNDYIFNFPDTSFAWAFVLALLAAALAARKRIAWWILVGYMIASAVWNVGGLVTSPKSVMDDVGEVVGIVFHVAAILVLLLARKQFWAKVRRAALVKAVATLIASMAVGTLIGWALLELFPGTLAREDRFLYALNRVSAFAGAASDTFSGHPHVFINALLGLFGALALMVTAIVLLA",
    "response#2": "RLHQVPHIAGLILGIFSVVVFLWSLSPTLRHFVHTPREWVDDYLFDAPDTSLAYALVIGLLAAAIASRKRIAWWLLTIYLTAFAILNAVVMVADRDVSAGIGLVVQVCAIGVLVASYNEFYTRVRRAAVWRALGVLLAGLAIGTLVGWGLVELFPGTLPSGERFLWSLNRVTALVYIDNEDFGGRPHGFVNTLLGLFGAMALLAAVITLGIQPHRPRWTVPRLSEVPHTVGLVLGVFATLCALWSISPGLRFITQVPRHYIDSYYFDAPDTSLVSALMVGLLAGAIATRKRIAWWLLVAYLAMFAVVNAFDVADEHDPNALAALVIHVGVIGLLIAAWPEFYTQVRRGALWKALGVLVAGVAVSWLLGWGLVELFPGTLPRGPERPLWAAARVVPAILFGPDNFDGHPPHFVNLLLGLLGAVALLAAAVVLAALVRWPGDPRSPVDLLELFGKNDSLGYFATRRDKAVVFAPNGRAAITYRVEVGVCLASGDPVGDPKAWQQAIAAWLALCQTYGWAPGVMGASSTGAEAFRQAGLNALQLGDEAILYPDTFRLSGPDMRAVRQAVTRAKRAGATVRIRRHRDLGSEEMAQVTARADAWRDTQTERGFSMALGRLGDSADGDCLLVEAVQNVAGEDQVVAMLSLVPWGNNGASLDLMRRSPQSPNGSIELMVSELCMQSEGIGVTRISLNFAMFRTAFEHGAQLGASPVARLWRGLLVFFSRWWQLETLYRSNMKYQPDWVPRYACYG",
    "response#3": "RERVAQWTGRLYLLAAVWSLITLPLRHTVVSDIGGDALGLVNVPSAPSLFIVCLLLALAGAIRRRLRAAHTVTVVLSAVGVLASALLFVGSIAHNDLLRDTHVRGFEREYVDFTNGVFFNTVALLIGVVALVLVVMSRREFPAKLAAGSRVAATAVLAGGLLVSFVVTLVITATARHTLHGSREVVRFALRSTFGISSDPSTPGMSGHTGYPWIYGLAGVMSTVALVLAILRAGGLVATVVQLAALFSVVLLLAGGRHGHLLNGIDMAFGVLSVPTSSSLVMVLLLVVLGAALRRRKKAALYVLVLFQVAGLVTTLVLQGLLLWAPDLLTLNARQMRHIPTQVGALAIADVISILLIVFLLSLRPAFPARLAPGAWWNGLTVLFVGFLGVIVLGWALTEVFPGHLGDTWERFAWVVNHATGENLQLRRIGVGEGPAWIDVVLDLGATFVSTAALYTLLFRKSTRHFRAGGLVATVVQLAALFSVVLLLAGGRHGHLLNGIDMAFGVLSVPTSSSLVMVLLLVVLGAALRRRKKAALYVLVLFQVAGLVTTLVLQGLLLWAPDLLTLNARQMRHIPTQVGALAIADVISILLIVFLLSLRPAFPARLAPGAWWNGLTVLFVGFLGVIVLGWALTEVFPGHLGDTWERFAWVVNHATGENLQLRRIGVGEGPAWIDVVLDLGATFVSTAALYTLRAGGLVATVVQLAALFSVVLLLAGGRHGHLLNGIDMAFGVLSVPTSSSLVMVLLLVVLGAALRRRKKAALYVLVLFQVAGLVTTLVLQGLLLWAPDLLTLNARQMRHIPTQVGALAIADVISILLIVFLLSLRPAFPARLAPGAWWNGLTVLFVGFLGVIVLGWALTEVFPGHLGDTWERFAWVVNHATGENLQLRRIGVGEGPAWIDVVLDLGATFVSTAALYTL"
  },
  {
    "reference": "MTEEQKPTSEKSVKRKSNTYWEWGKAIIIAVALALLIRHFLFEPYLVEGSSMYPTLHDGERLFVNKSVNYIGEIERGDIVIINGDTSKVHYVKRLIGKPGETVEMKNDTLYINGKKIAEPYLASNKKEAKKLGVNLTGDFGPVKVPKGKYFVMGDNRLNSMDSRNGLGLIAENRIVGTSKFVFFPFHDMRQTK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should be able to enhance the efficiency of signal peptide processing.2. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.3. A key design requirement for the protein is the optimization of the active site to achieve high catalytic efficiency for Cleavage of hydrophobic, N-terminal signal or leader sequences   from secreted and periplasmic proteins..4. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "FKDAGKPVIAYADNYSQGQYYLASFADEIYLNSIGSVDIHGLSQENLYFKEMLDKLAVTPHIFRVGTYKSAVEPFLRNDMSAEAKANMQRWLGEMWNNYVLSVSENRNIKKDRILPNAKQYLAELKALKGNSTAYAQQRGLVTDVVTRLDLDKKI",
    "response#2": "FKDAGKPVIAYADNYSQGQYYLASFADEIYLNSIGSVDIHGLSQENLYFKEMLDKLAVTPHIFRVGTYKSAVEPFLRNDMSAEAKANMQRWLGEMWNNYVLSVSENRNIKKDRILPNAKQYLAELKALKGNSTAYAQQRGLVTDVVTRLDLDKKL",
    "response#3": "FKDAGKPVIAYADNYSQGQYYLASFADEIYLNSIGSVDIHGLSQENLYFKEMLDKLAVTPHIFRVGTYKSAVEPFLRNDMSAEAKANMQRWLGEMWNNYVLSVSENRNIKKDRILPNAKQYLAELKALKGNSTAYAQQRGLVTDVVTRLDLDKKK"
  },
  {
    "reference": "MSAFIVLGSQWGDEGKGKMTDYLAKDVDVVVRFQGGNNAGHTVRVGDKEYKLHIIPSGILYKDKVNVIGNGVVLDPEALFQEIDYLEKAGVDIQPDNLMISDRAQLIMPYHKVLDGIKERSRGKKDIGTTGKGIGPCYTDKMERSGIRVCDLINQDVFKENLKENLKIKNDIITKVYGEEALDFDSICDQYIEYRKKLAPYVKDISVEVYNSIKNDKKVLFEGAQGTLLDIDYGTYPYVTSSSTIAGGVCIGAGIGPTLITGAIGVAKAYTTRVGKGPFPTELFDDTGDWIRKSGREYGVTTGRARRCGWLDLVILKTSSRVSGLTNFAITKIDTLGGLEKVKVCTGYKFNGKIIDYVPASLQDLAKCEPVYEEFDGWDKDIENAKTYDELPENAKIYLNKIEEFTNTKISIVSVGPGRDQTINLNNM",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The GTP, substrate, Mg(2+), IMP binding site should be stable and able to withstand changes in environmental conditions.2. For general function, the protein need meet that Plays an important role in the de novo pathway of purine nucleotide biosynthesis; Catalyzes the first committed step in the biosynthesis of AMP from IMP.3. A key design requirement for the protein is the optimization of the Proton donor, Proton acceptor to achieve high catalytic efficiency for GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-   dicarboxyethyl)-AMP + phosphate.4. The protein must bind to Mg(2+) in order to perform its enzymatic function.5. The designed protein should interact with enzymes and substrates within the Purine metabolism, AMP from IMP: step 1/2, AMP biosynthesis via de novo pathway pathway to enhance or regulate its activity.6. A protein that can perform adenylosuccinate synthase activity, GTP binding, magnesium ion binding in cytoplasm to support 'de novo' AMP biosynthetic process. The designed protein sequence is ",
    "response#1": "MIASSIEEAIDDIRQGKMVILVDDEDRENEGDLVMAAEKVTPQAINFMAKYGRGLICLTLTGEKCDELGLELMVGNNTALHETAFTISVDARTGVSTGISAHDRATTILTAVADDTRPTDLARPGHIFPLRARPGGVLRRAGHTEAAVDLARLAGLYPAGALIEIMNEDGTMARLPKLVEIAKKFNLKIITIADLIEYRRRRETLIKREC",
    "response#2": "MIDLNIEDAINQIKNGKMIVIVDDEDRENEGDLLMAAEKVTPESINFMATHGRGLICTPMLADRLDELQIPHQVEDNTSFLGTAFTVSVDAREGVTTGISAADRARTIQVLIDPKTKPENLGKPGHIFPLVAKDGGVLRRAGHTEAAVDLARLAGLYPAGVLVEIMNEDGTMARVPELFAIAKKFGLKIISIADLIEYRRRKESLIEKGDIAIPIKMNTKPEEFRYLRTKQEKMGHDLKEIEIPESNTNYIYLKTKKEKLGHYIFCEENEE",
    "response#3": "MQWGDEGKGKIVDFLTKDAKIVVRYQGGNNAGHTIVVGDKKYLLHLVPSGILRPNVINVIGNGVVVNPKVLFEEIDALEAGGIETTERLMVSESAHIIMPYHKKLDAIAEEARGKQKIGTTRRGIGPAYSSKASRNGIRIADLLDKETFAEKLKENLEEKNRILKKLYNAEAIEFEQILDRYYEYAEQLKPFVTDTSVILNEALDNDKNILFEGAQGTLLDIDHGTYPFVTSSSTIASGACTGLGVGPSKIDRVIGVVKAYTTRVGEGPFPTELFDETGEKLGEKLREVGHEFGTTTGRPRRVGWFDAVVARQAVRVNGLTGLCMTKLDVLDGLKEVKICTSYKLDGKEIDYFPASLETLEKCKPIYETIEGWDTDISAVKKYEDLPENAKKYIERIEELVGTKVSMVSTGPERDETILKSV"
  },
  {
    "reference": "MMLLLPLIIVLMMKLSDARTHSLRYFRLGISEPGYGIPEFISAGYVDSHPITMYNSVSQLKEPRALWMEENLAPDHWERYTQLLRGWQQAFKVELKQLQHHYNHSGFHTYQRMIGCELLEDGSITGFLQYAYDGQDFLIFNKDTLSWMAMDNVADIIRRVWEANRHELQYQKNWLEEECIAWLKRFLEYGKDALQRTEPPKVRVNHKETFPGITTLYCRAYGFYPPEISINWMKNGEEIFQDTDYGGILPSGDGTYQTWVSVELDPQNGDIYSCHVEHGGVHMVLQGFQESETILLVVKAVGFIVLAIALAGVGILAWRKRPRGKNKVICLSTPEH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that can perform T cell receptor binding, beta-2-microglobulin binding in MHC class I protein complex, early endosome membrane, plasma membrane, extracellular space, late endosome membrane to support positive regulation of T cell mediated cytotoxicity directed against tumor cell target, antigen processing and presentation of peptide antigen via MHC class I, defense response to Gram-negative bacterium, defense response to Gram-positive bacterium, innate immune response.2. The protein should have a high affinity for 5-(2-oxoethylideneamino)-6-(D-ribitylamino)uracil.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.4. Ensure the presence of a signal peptide within the protein.5. The protein design should prioritize the formation of strand-strand-strand-strand-helix-helix-strand-strand-strand-strand-turn-strand-helix-helix-helix elements.6. The Extracellular topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "AAEEQLSFRMLQTSSFANHSWAHSEGSGWLGDLQTHGWDTVLGTIRFLKPWSHGNFSKQELKNLQSLFQLYFHSFIQIVQASAGQFQLEYPFEIQILAGCRMNAPQIFLNMAYQGSDFLSFQGISWEPSPGAGIRAQNICKVLNRYLDIKEILQSLLGHTCPRFLAGLMEAGESL",
    "response#2": "AAEEQLSFRMLQTSSFANHSWAHSEGSGWLGDLQTHGWDTVLGTIRFLKPWSHGNFSKQELKNLQSLFQLYFHSFIQIVQASAGQFQLEYPFEIQILAGCRMNAPQIFLNMAYQGSDFLSFQGISWEPSPGAGIRAQNICKVLNRYLDIKEILQSLLGHTCPRFLAGLMEAGEST",
    "response#3": "PLLLLLLLETGAQDVRVQVLPEVRGQLGGTVELPCHLLPPVPGLYISLVTWQRPDAPANHQNVAAFHPKMGPSFPSPKPGSERLSFVSAKQSTGQDTEAELQDATLALHGLTVEDEGNYTYACRVRFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWPKTHVTHHPGSEGDVTLRCWALGFYPADITLTWQLNGEELTQDMELVETRPAGDGTFQKWASVVVPLGKEQNYTCHVYHEGLPEPLTLTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPTGQSVAYSIRSTARVVLDPWDVRSQVICEVAHVTLQGLPSAPVVLGPAARTTPEHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPTGQSVAYSIRSTARVVLDPWDVRSQVICEVAHVTLQGDPLRGTANLSVQAPAADRPPKVSLMPATLARAAPGEAPPELLCLVSHFYPSGGLEVEWELRGGPGGRSQKAEGQRWLSALRHHSDGSVSLSGHLQPPPVTTEQHGARYACRIHHPSLPASGRSAEVSVRVTGP"
  },
  {
    "reference": "MLKGMRILLGISGGIAAYRAAELARLMIRQGAEVRCVMTQGACEFITPLTLEALTGQQVHSELFDLTREREMGHINLARWADVLVVAPATADIMARFAHGICDNLLSTLYQARRGPVLLAPAMNTVMWQAPATQRNVQALQQDGVHIVGPAVGDLACGETGAGRMSEPMDIVAGVYGLLLDQSLAGQRWVINAGPTREAWDDVRTLSNRASGLLGSLIAEEATMRGAVVQLIAGPGTPATPGVQQRRDVESAEDMLAACVRAAVGADLFIASAAVSDYRFAEPINGKLKRGAANDGNGLTVQLLENTDIVATIAGMDNRPETVVAFAAEATDHLENARGKLRAKGVDAIFANDISRMGGSRGGGWWLTADSECKSGEMGKAELAAWIVQRVIDMQSGKQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.2. The design of the protein must focus on the precise arrangement of amino acids in the Proton donor to enable effective catalysis of H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.3. The designed protein must have at least one Flavoprotein domain.4. The protein should be able to bind CTP ligand in a variety of conditions5. The designed protein should have FMN binding to facilitate coenzyme A biosynthetic process. The designed protein sequence is ",
    "response#1": "MNILLGVSGSIAAYKSPDLVRRLKEVGFDVRVVMTDGGRSFVTPLTLQAVSGYPVVYDLLDPAHEAGMGHIELARWADLILIAPTSANMIAKLAGGFADDLLSAICLATEKPIMLAPGMNRLMWANQAVQENVAKLEARGMILLAPESGSQACGEIGQGRMPEPLQIRDAEHIAALLEDYLAGRRVVISAGGTREALDPVRYLGNSSSGRMGAALAADAAARGARVTMVTAHIEVPVPSCVRQVPVSSTEDLDEAMRREATGADIVVMAVAAADFAPSSVSDLKIKKHEGDRRVGDRMSLDLIQTPDVLAGLAAARTPGQVVVGFAAETAPDRDELLALARAKLARKGCDLLAVNDVSSGRVFGKVGTDVTVRYPDGTREELPRMSKAEMADHILDHLLG",
    "response#2": "MNILLGVSGSIAAYKSPDLVRRLKEVGFDVRVVMTDGGRSFVTPLTLQAVSGYPVVYDLLDPAHEAGMGHIELARWADLILIAPTSANMIAKLAGGFADDLLSAICLATEKPIMLAPGMNRLMWANQAVQENVAKLEARGMILLAPESGSQACGEIGQGRMPEPLQIRDADGVLAALAAHFQARPGLAGRRVLVTAGRTEETLDPVRYLTNKSSGKMGFALAQEAFARGARVTLIHGPVELPLPAGVKNVSVTSAAEMFEAVQRNLGDSDIYLSAAAIADYTPEQFSEQKIKKQAGNFSLSLKRTVDILKFAGAKKKAGQQIVGFAVETEADESNAVKKLADKNLDLVVLNNPLEEGAGFRHDTNRVTLIDRAGAVEDWPRLSKREVAERLLDRAARALKA",
    "response#3": "MNILLGVSGSIAAYKSPDLVRRLKEVGFDVRVVMTDGGRSFVTPLTLQAVSGYPVVYDLLDPAHEAGMGHIELARWADLILIAPTSANMIAKLAGGFADDLLSAICLATEKPIMLAPGMNRLMWANQAVQENVAKLEARGMILLAPESGSQACGEIGQGRMPEPLQIRDPAGIAAAVAAAFAPTDLAGRRVLVTAGRTEETLDPVRYLTNKSSGKMGFALAQEAFARGARVTLIHGPVELPLPAGVKNVSVTSAAEMFEAVQRNLGDSDIYLSAAAIADYTPEQFSEQKIKKQAGNFSLSLKRTVDILKFAGAKKKAGQQIVGFAVETEADESNAVKKLADKNLDLVVLNNPLEEGAGFRHDTNRVTLIDRAGAVEDWPTLPKAQVAARLMARAAELLGA"
  },
  {
    "reference": "MQQIEVKRLLVNYKTLEEFRNFREFGAAELSMKDDLEANIIENDSESPFYGIYFGGKLVARMSLYRIDGKYDRYFEPPQDYLELWKLEVLEPYRGKGFGRALVDFAKSFNLPVKTNARQRSNEFWTKMGFEPVTYQTDRDRGESPYVWYPEGVKEQLSEDEGSVETLEN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must have at least one N-acetyltransferase domain.2. The protein must exhibit acyltransferase activity, transferring groups other than amino-acyl groups. The designed protein sequence is ",
    "response#1": "IRIRAAKPIDFPKVAAMHYPVWRQSWTGILDPYLLDMIGSPKLWVEESYPQSLKRGGWSMWIAESGGQPIGMTMFGPDIAHPDRIQIDALYVAENSQRHGIGGRLLNRALHSHPSADMILWCAEKNSKARGFYEKKDFHIDGRTFTWKPLSGVNVPHVGYRLYRSAA",
    "response#2": "LEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSDSPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELIDFAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKQ",
    "response#3": "LDINYKTDELFEDFRNFGNKDLYLVDELRGEMIDASSDSPFYGIYVGDCLGARMALYRKGEVEETHFPGFDDYNVIWKLEVLRDFQGRGYGTSLLDFAKDQGLPIKVIARNQSKDFFIKQGFTDLEETNRDGHDVLVWTC"
  },
  {
    "reference": "MHELSLCESLVSLLEEEMQKLGCGALKKVVLQVGPLALIEEGAMRFAFEVASKGTVVEGAELFIEWIAVRGYCLDCQKEALVYKIGESCPCCGGVMILSEEGKDLKVASIEVE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Involved in the maturation of [NiFe] hydrogenases2. The protein must have high specificity towards zinc ion binding.3. The protein should be able to bind Zn(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "FISPGWVDLHVHIWHGGTDISIRPSECGAERGVTTLVDAGSAGEANFHGFREYIIEPSRERIKAFLNLGSIGLVACNRVPELRDIKDIDLDRILECYAENSEHIVGLKVRASHVITGSWGVTPVKLGKKIAKILKVPMMVHVGEPPALYDEVLEILGPGDVVTHCFNGKSGSSIMEDEDLFNLAERCAGEGIRLDIGHGGASFSFKVAEAAIARGLLPFSISTDLHGHSMNFPVWDLATTMSKLLSVDMPFENVVEAVTRNPASVIRLDMENRLDVGQRADFTVFDLVDADLEATDSNGDVSRLKRLFEE",
    "response#2": "FISPGWVDLHVHIWHGGTDISIRPSECGAERGVTTLVDAGSAGEANFHGFREYIIEPSRERIKAFLNLGSIGLVACNRVPELRDIKDIDLDRILECYAENSEHIVGLKVRASHVITGSWGVTPVKLGKKIAKILKVPMMVHVGEPPALYDEVLEILGPGDVVTHCFNGKSGSSIMEDEDLFNLAERCAGEGIRLDIGHGGASFSFKVAEAAIARGLLPFSISTDLHGHSMNFPVWDLATTMSKLLSVDMPFENVVEAVTRNPASVIRLDMENRLDVGQRADFTVFDLVDADLEATDSNGDVSRLKRLFG",
    "response#3": "FISPGWVDLHVHIWHGGTDISIRPSECGAERGVTTLVDAGSAGEANFHGFREYIIEPSRERIKAFLNLGSIGLVACNRVPELRDIKDIDLDRILECYAENSEHIVGLKVRASHVITGSWGVTPVKLGKKIAKILKVPMMVHVGEPPALYDEVLEILGPGDVVTHCFNGKSGSSIMEDEDLFNLAERCAGEGIRLDIGHGGASFSFKVAEAAIARGLLPFSISTDLHGHSMNFPVWDLATTMSKLLSVDMPFENVVEAVTRNPASVIRLDMENRLDVGQRADFTVFDLVDADLEATDSNGDVSRLKRLFDE"
  },
  {
    "reference": "MLRFAPSPTGDMHIGNLRAAIFNYIVAKQQHKPFLIRIEDTDKERNIEGKDQEILEILKLMGISWDKLVYQSHNIDYHREMAEKLLKENKAFYCYASAEFLEREKEKAKNEKRPFRYLDEWATLEKDKNHAPVVRLKAPNHAVSFNDAIKKEVEFEPDELDSFVLLRQDKSPTYNFACACDDLLYEISLIIRGEDHVSNTPKQILIQEALGSNDPIVYAHLPIILDEVSGKKMSKRDEASSVKWLLNQGFLPVAIANYLITIGNKVPKEVFSLDEAIEWFSLENLSNSPAHFNLKYLKHLNHEHLKLLDDEKLLKLTSIKDKNLLGLLRLFIEECGTLLELKEKISLFLEPKDIVKTYENEDFKERCLVLFNALKSMDFQAYKDFESFKKEAMRLSQLKGKDFFKPLRILLTGNSHGVELPLIFPYIQSHYQEILRLKA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be designed to have high stability and activity towards ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-   glutamyl-tRNA(Glu).2. The designed protein should have a high specificity for ATP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "LAEKARPHVEAAGYDVSDDRLQTICGLVQDRIQVVPEVVTDNRYFFEDPEAYEEAGVEKRWNEESADLLLAYADRLEGVDAFDTDTVETELRDLADEKDVGAGAIIHPSRLAVSGRSYGPGVFGLLAAVGKEACIRRMHRR",
    "response#2": "MVKTRFAPSPTGYLHIGGARTALFSWAFARKQGGKFVLRIEDTDLERSTQQSVQAILDGMAWLGLDYDEGPYYQMQRLNRYQEVAEQLLTQGLAYQCYASREELDALREQQRLAGLKPRYDGRWRDSRQTPPAGVKPVVRLKTPQHGYVVFDDLVKGKISVANHELDDLVLLRSDGTPTYNFGVVLDDLDMGITHVIRGDDHVNNTPRQINILKALGAAIPQYAHVPMILGADGERLSKRHGAVSVMHYRDQGYLPEALINYLARLGWSHGDEEIFSREQLVEWFDLAAISRSPAKFNPEKLTWLNQHYLKS",
    "response#3": "MVKTRFAPSPTGYLHIGGARTALFSWAFARKQGGKFVLRIEDTDLERSTQQSVQAILDGMAWLGLDYDEGPYYQMQRLNRYQEVAEQLLTQGLAYQCYASREELDALREQQRLAGLKPRYDGRWRDSRQTPPAGVKPVVRLKTPQHGYVVFDDLVKGKISVANHELDDLVLLRSDGTPTYNFGVVLDDLDMGITHVIRGDDHVNNTPRQINILKALGAAIPQYAHVPMILGADGERLSKRHGAVSVMHYRDQGYLPEALINYLARLGWSHGDEEIFSREQLVEWFDLAAISRSPAKFNPEKLTWLNQHYLKA"
  },
  {
    "reference": "MANLRDIRKKIGSVKNTQKITHAMKLVSTSKLRKAEEVARNSRAYALKLDAVFDDVLSKMKNQGIEDIQSKYFRELERLEIKKVDIIFITADKGLCGGFNTNTIKKVLACTNEYKEKDIKVRLRGIGKKGNEYFSFNGIEVLDKINNLSSMPNYERVQEFMKKVVEDYLSGKTDKVIIIHNGFKNMITQEIRVKTILPIGYKIIHQNPQPSETQETITSEPSGSEDEILDSLAEKYVEYSLYYALIDSLAAEHSARMQAMDTATNNAKDLVKTLTISYNKARQEAITTELVEINAGVEALK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that can perform ATP binding in plasma membrane, proton-transporting ATP synthase complex, catalytic core F(1) to support proton motive force-driven ATP synthesis, proton motive force-driven plasma membrane ATP synthesis.2. For general function, the protein need meet that Produces ATP from ADP in the presence of a proton gradient across the membrane The designed protein sequence is ",
    "response#1": "GQRVFDTFFPLGMGGTAAIPGGFGAGKTMTQHQLAKWSSADIIVYVGCGERGNEMTDVLEEFPKLEDPSTGKSMMERTVLIANTSNMPVAAREASIYTGITIAEFYRDMGYNVALMADSTSRWAEALREISGRLEEMPAEEGFPAYLPSRLAEFYERAGYVKTLGQDREGSISLIGAVSPPGGDFSEPVTQNTKRYVRCFWALDKSLASARHFPAVNWLESYT",
    "response#2": "VNPLDRDREWTVSVKVKLGDRVKPGQVVAEVPETSIVTHRVMVPPHLSGEVVEVVPNGKYTVDQEIVTVKDDKGNNHQIRLHQKWPVK",
    "response#3": "GQRVFDTFFPLGMGGTAAIPGGFGAGKTMTQHQLAKWSSADIIVYVGCGERGNEMTDVLEEFPKLEDPSTGKSMMERTVLIANTSNMPVAAREASIYTGITIAEFYRDMGYNVALMADSTSRWAEALREISGRLEEMPAEEGFPAYLPSRLAEFYERAGYVKTLGQDREGSISLIGAVSPPGGDFSEPVTQNTKRYVRCFWALDKSLASARHFPAVNWLESYNGQRVFDTFFPLGMGGTAAIPGGFGAGKTMTQHQLAKWSSADIIVYVGCGERGNEMTDVLEEFPKLEDPSTGKSMMERTVLIANTSNMPVAAREASIYTGITIAEFYRDMGYNVALMADSTSRWAEALREISGRLEEMPAEEGFPAYLPSRLAEFYERAGYVKTLGQDREGSISLIGAVSPPGGDFSEPVTQNTKRYVRCFWALDKSLASARHFPAVNWLESYSFIDL"
  },
  {
    "reference": "MPYNTIDEALEALRAGELVLVTDDPDRENEGDLICAAEFADTERVNFMATHAKGLICMPMSEAICRRLALPQMVPENTDNHQTAFTVSIDHISTTTGISAGERSVTALRCADPGARPEEFRRPGHMFPLLARRNGVLERAGHTEATVDLLRLAGLEECGLCCEIMRQDGGMMRTTELLEFAEHWGLRMITIRGLQEYRKRRDKLVERVADAALPTRYGSFRAVGFINRLNGEHHVALVKGEIGNGENLLCRVHSECLTGDAFGSIRCDCGGQLAAAMAQIEREGRGILLYLRQEGRGIGLINKLRAYELQDRGMDTLEANLALGFGGDEREYYIGAQILRELGAKTLRLLTNNPQKIYDLSHFGMEITQRVPIEMPATEQNLFYLQTKQHKMGHLLHYGD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be able to regulate riboflavin biosynthetic process.2. The Nucleophile; for GTP cyclohydrolase activity active site of the designed protein must be optimized for substrate binding and specificity.3. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.. The designed protein sequence is ",
    "response#1": "SIGLPTEDYGDLTIKLFESCLDQHHHLALIKDNPKQTDQSTLVRIHSECFTGDIFGSARCDCGWQLHESLRQISQQGGTLLYLRQEGRGIGLLNKLKAYALQDQGFDTVEANQKLGFGSDERDYWIGAQILRQLKQLTVKLLTNNPQKIADLKQQGITVIERIK",
    "response#2": "SIGLPTEDYGDLTIKLFESCLDQHHHLALIKDNPKQTDQSTLVRIHSECFTGDIFGSARCDCGWQLHESLRQISQQGGTLLYLRQEGRGIGLLNKLKAYALQDQGFDTVEANQKLGFGSDERDYWIGAQILRQLKQLTVKLLTNNPQKIADLKQQGITVIERIE",
    "response#3": "SIGLPTEDYGDLTIKLFESCLDQHHHLALIKDNPKQTDQSTLVRIHSECFTGDIFGSARCDCGWQLHESLRQISQQGGTLLYLRQEGRGIGLLNKLKAYALQDQGFDTVEANQKLGFGSDERDYWIGAQILRQLKQLTVKLLTNNPQKIADLKQQGITVIERIA"
  },
  {
    "reference": "MTAETNTNKHPLYIPYAGYTLLELPLLNKGSAFTQEERSHFNLHGLIPHVIETIEEQSQRSYQQYGAFNDDINKHIYLRNIQDTNETLFYRLINDHLEEMMPIIYTPTVGEACQRFSDIYRRHRGIFISYPDREHIDDILQNVSKRNVKVIVITDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPITIDVGTNNQQLLNDPIYMGWRQPRISGDEYYEFIDQVLTGIRRRWPHALIQFEDFAQKNAMPLLTKYRDKFCCFNDDIQGTAAVSVGSLIAASRAAGKQLKDQTITFLGAGSAGCGIAEQIVAQMVAEGLTDAQARARVYMVDRFGLITENQPNLLDFQRKLAQKAEVVSDWGNVEEVISLLDVVKNAKPTVLIGVSGQPGLFTEEVIRTLAENCERPIVMPLSNPTSRVEALPSDVIQWTNGRALIATGSPFAPVNYQGKLYNISQCNNSYIFPGIGLGVIASGAKRVTDNMLMASSNALADCSPLLQNPNADLLPAIADIQDVSKRIAFKVAKAAIEDGVALNMSDEVLLQNIEKEFWKPKYRGYKRVPF",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The presence of Mg(2+), Mn(2+) is crucial for the protein to function optimally under specific conditions.2. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.3. The designed protein must be able to perform oxaloacetate decarboxylase activity under specific conditions.4. The designed protein should be able to compete with endogenous proteins or inhibitors for a divalent metal cation binding. The designed protein sequence is ",
    "response#1": "QDRNEKLFYRVLTSDIEKFMPIVYTPTVGLACQQYSLVFRKPRGLFITIHDRGHIASVLNAWPEDVIKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGMNPQECLPVILDVGTENEELLKDPLYIGLRQRRVRGSEYDDFLDEFMEAVSSKYGMNCLIQFEDFANVNAFRLLNKYM",
    "response#2": "IQGTAAVTLGTLIAASRAAGSQLSEQKIVFLGAGSAGCGIAEQIIAWMRTEGGLSDEQARARVFMVDRFGLLTDNMPNLLSFQSKLVQKRDSLKGWDTQSDSISLLDVVRNAKPDILIGVSGQTGLFTEEIIREMHKHCARPIVMPLSNPTSRVEATPHDILNWTDGAALVATGSPFQPVTVKEKTYPIAQCNNAYIFPGIGLGIISSGALRVTDEMMMAASEALASHSPLVNTGSGLVLPPLTDIQQVSKDIAFAVGKMY",
    "response#3": "IQGTAAVTLGTLIAASRAAGSQLSEQKIVFLGAGSAGCGIAEQIIAWMRTEGGLSDEQARARVFMVDRFGLLTDNMPNLLSFQSKLVQKRDSLKGWDTQSDSISLLDVVRNAKPDILIGVSGQTGLFTEEIIREMHKHCARPIVMPLSNPTSRVEATPHDILNWTDGAALVATGSPFQPVTVKEKTYPIAQCNNAYIFPGIGLGIISSGALRVTDEMMMAASEALASHSPLVNTGSGLVLPPLTDIQQVSKDIAFAVGKML"
  },
  {
    "reference": "MGVEGCTKCIKYLLFVFNFVFWLAGGVILGVALWLRHDPQTTTLLYLELGDKPAPSTFYVGIYILIAVGAVMMFVGFLGCYGAIQESQCLLGTFFTCLVILFACEVAAGIWGFVNKDQIAKDVKQFYDQALQQAVMDDDANNAKAVVKTFHETLNCCGSNTLTTLTTAVLRNSLCPSSSNSFTQLLKEDCHQKIDELFSGKLYLIGIAAIVVAVIMIFEMILSMVLCCGIRNSSVY",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should contain Extracellular topological domains.2. The protein must exhibit the following characteristics: Also involved in cell adhesion and motility; May regulate the compartmentalization of enzymatic activities; In T cells, defines the subcellular localization of dNTPase SAMHD1 and permits its degradation by the proteasome, thereby controlling intracellular dNTP levels ; In myoblasts, associates with CD9 and PTGFRN and inhibits myotube fusion during muscle regeneration ; Can act both as positive and negative regulator of homotypic or heterotypic cell-cell fusion processes; In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles .3. The designed protein should have integrin binding, virus receptor activity, cholesterol binding, transferrin receptor binding within vesicle to regulate cellular response to low-density lipoprotein particle stimulus, positive regulation of B cell receptor signaling pathway, humoral immune response mediated by circulating immunoglobulin, positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell.4. The designed protein should have a binding site that is specific to the target cholesterol ligand. The designed protein sequence is ",
    "response#1": "MRLVNSAGHRCLGRVEIKFQGKWGTVCDDNFSKDHASVICKQLGCGSAISFSGSAKLGAGSGPIWLDDLACNGNESALWDCKHRGWGKHNCDHAEDVGVICPVLKVGVAMRFSPVEVAKAVYRCWEEKPSALEAGDATVCLTIQKSSLDQLGDIQSSVRFDLALDPGRLTSRAIFNETKNPTLTRRKTLGLGIHCETLKLLLPDCVEDVVSPIILHLNFSLVREPIPSPQNLRPVLAVGSQDLFTASLPFEPRLVGGEIPCSGRVEVKHGDVWGSVCDFDLSLEAASVVCRELQCGTVVSILGGAHFGEGSGQIWGEEFQCSGDESHLSLCSVAPPLDRTCTHSRDVSVVCSPPEKPSPLLKVGISIRFAPSEVAKTVYQCWGRTPTVLEAGEATVCLTVRKGSPDLLGDVQSSVRYDLALDPGRLISRAIFDETKNCTLTRRKTLGLGDHCETMKLLLPDCVEDAVTPIILRLNLSLAGDSAPSRNLRPVLAVGSQDHS",
    "response#2": "EMDIAFLIDGSGSIDQSDFTQMKDFVKALMGQLASTSTSFSLMQYSNILKTHFTFTEFKSSLSPQSLVDAIVQLQGLTYTASGIQKVVKELFHSKNGARKSAKKILIVITDGQKFRDPLEYRHVIPEAEKAGIIRYAIGVGDAFREPTALQELNTIGSAPSQDHVFKVGNFVALRSIQRQIQEKIKKKPSHDIVFLIDGSGSIDQNDFNQMKGFVQAVMGQFEGTDTLFALMQYSNLLKIHFTFTQFRTSPSQQSLVDPIVQLKGLTFTATGILTVVTQLFHHKNGARKSAKKILIVITDGQKYKDPLEYSDVIPQAEKAGIIRYAIGVGHAFQGPTARQELNTISSAPPQDHVFKVDNFAALGSIQKVLEQNADGVQDIAFLIDGSGSIDQSDFTQMKDFVKALMGQLASTSTSFSLMQYSNILKTHFTFTEFKSSLSPQSLVDAIVQLQGLTYTASGIQKVVKELFHSKNGARKSAKKILIVITDGQKFRDPLEYRHVIPEAEKAGIIRYAIGVGDAFREPTALQELNTIGSAPSQDHVFKVGNFVALRSIQRQPVLKVGVAMRFSPVEVAKAVYRCWEEKPSALEAGDATVCLTIQKSSLDQLGDIQSSVRFDLALDPGRLTSRAIFNETKNPTLTRRKTLGLGIHCETLKLLLPDCVEDVVSPIILHLNFSLVREPIPSPQNLRPVLAVGSQDLFTASLPFERGM",
    "response#3": "PLLKVGISIRFAPSEVAKTVYQCWGRTPTVLEAGEATVCLTVRKGSPDLLGDVQSSVRYDLALDPGRLISRAIFDETKNCTLTRRKTLGLGDHCETMKLLLPDCVEDAVTPIILRLNLSLAGDSAPSRNLRPVLAVGSQDHLDPVLKVGVAMRFSPVEVAKAVYRCWEEKPSALEAGDATVCLTIQKSSLDQLGDIQSSVRFDLALDPGRLTSRAIFNETKNPTLTRRKTLGLGIHCETLKLLLPDCVEDVVSPIILHLNFSLVREPIPSPQNLRPVLAVGSQDLFTASLPFEPVLKVGVAMRFSPVEVAKAVYRCWEEKPSALEAGDATVCLTIQKSSLDQLGDIQSSVRFDLALDPGRLTSRAIFNETKNPTLTRRKTLGLGIHCETLKLLLPDCVEDVVSPIILHLNFSLVREPIPSPQNLRPVLAVGSQDLFTASLPFEPVLKVGVAMRFSPVEVAKAVYRCWEEKPSALEAGDATVCLTIQKSSLDQLGDIQSSVRFDLALDPGRLTSRAIFNETKNPTLTRRKTLGLGIHCETLKLLLPDCVEDVVSPIILHLNFSLVREPIPSPQNLRPVLAVGSQDLFTASLPFEEPTPPTSVAPVLKVGVAMRFSPVEVAKAVYRCWEEKPSALEAGDATVCLTIQKSSLDQLGDIQSSVRFDLALDPGRLTSRAIFNETKNPTLTRRKTLGLGIHCETLKLLLPDCVEDVVSPIILHLNFSLVREPIPSPQNLRPVLAVGSQDLFTASLPFE"
  },
  {
    "reference": "MKTTLLKTLTPELHLVQHNDIPVPSLKTCGWNTKNFPCKGHSLSVGXPQNAKQDVLWLSEVEPFKNGNAIRGGVPICYPWFGGVKQPAHGTARIRLWQLSHYYISVHKVRLEFELFSDLNIIEAKVSMVFTDKCHLTFTHYGEESAQAALHTYFNIGDINQVEVQGLPETCFNSLNQQQENVPSPRHISENVDCIYSAENMQNQILDKSFNRTIALHHHNASQFVLWNPWHKKTSGMSETGYQKMLCLETARIHHLLEFGESLSVEISLKG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be involved in carbohydrate metabolic process by localizing to cytoplasm and exhibiting glucose-6-phosphate 1-epimerase activity.2. The protein's active site must be carefully engineered to ensure that the amino acids involved in alpha-D-glucose 6-phosphate = beta-D-glucose 6-phosphate are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "MVVLPYRDRLQVFSKYLQQLVMESLGKKFNRKGEVVNQGISVFGNKGSTDQHAYVQQLRDGIDNFFCIFIELLDSPSTSIFDKKENPKEYLSGFLQGTRSALSSENRQSITITLEKLNCFSLGALIALFERAVSFYAELVNINAYDQPGVEAGKKAAANFL",
    "response#2": "ESHAILPYDHYLRSLPAYLEQADMESNGKSVDRDGRVVDYPTGQIIWGATGINGQHAFYQLLHQGTKMIPADFIVSITPHTELQEHHDILIANFLAQTEALMRGRTREETLEEMGRSPGDPSVQHRVFEGNHPSNAILLKKLTPHTLGMLIALYEHKIFVEGVIWNLNSYDQWGVELGKQLAGRKARW",
    "response#3": "MVVLPYRDRLQVFSKYLQQLVMESLGKKFNRKGEVVNQGISVFGNKGSTDQHAYVQQLRDGIDNFFCIFIELLDSPSTSIFDKKENPKEYLSGFLQGTRSALSSENRQSITITLEKLNCFSLGALIALFERAVSFYAELVNINAYDQPGVEAGKKAAANL"
  },
  {
    "reference": "MFSRTASKFRNTRRLLSTISSQIPGTRTSKLPNGLTIATEYIPNTSSATVGIFVDAGSRAENVKNNGTAHFLEHLAFKGTQNRSQQGIELEIENIGSHLNAYTSRENTVYYAKSLQEDIPKAVDILSDILTKSVLDNSAIERERDVIIRESEEVDKMYDEVVFDHLHEITYKDQPLGRTILGPIKNIKSITRTDLKDYITKNYKGDRMVLAGAGAVDHEKLVQYAQKYFGHVPKSESPVPLGSPRGPLPVFCRGERFIKENTLPTTHIAIALEGVSWSAPDYFVALATQAIVGNWDRAIGTGTNSPSPLAVAASQNGSLANSYMSFSTSYADSGLWGMYIVTDSNEHNVQLIVNEILKEWKRIKSGKISDAEVNRAKAQLKAALLLSLDGSTAIVEDIGRQVVTTGKRLSPEEVFEQVDKITKDDIIMWANYRLQNKPVSMVALGNTSTVPNVSYIEEKLNQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have a high affinity for Zn(2+).2. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of Release of N-terminal transit peptides from precursor proteins   imported into the mitochondrion, typically with Arg in position P2..3. For general function, the protein need meet that Preferentially, cleaves after an arginine at position P2 .; Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins4. Zn(2+) is required for the proper folding and stability of the protein.5. A protein that can perform metal ion binding in mitochondrion, mitochondrial matrix to support protein processing involved in protein targeting to mitochondrion. The designed protein sequence is ",
    "response#1": "MRAAPNEEPRRKLYLAANSSTPEQIHVLETLLKKRAELAQLVGRDSFAHMTLDDKMAKKPEHVTNFLDALIDHTRPFARNALRTLAQRKQAHHNLPALPVIQAWDRDFYCPPDPPAPPIPLPPLTIGTVFMGLSRLFRHLYGVSLRPAQAASGEVWHPDVQKLEVVDEQQGIIGWIYADLFPRRGKASGAAHYTVRCSRRTDDDDEANDGMFEGTELQIQESQQFEAVKRHRLPNQEGVYQLPLVVLLTEFARPSLSKGAAVLEWHEVQTLFHEMGHAMHSMLGRTEYQNVSGTRCATDFVELPSILMEHFLNSPAVLSLFDADNTTSLRQIGNHHNDPCHAIDTYSQIMLAVVDQVYHSPSVLNSSFDSTNEFANLVNKRGLIPYVPGTSFQTQFGHLFGYGATYYSYLFDRAIASRVWSKVFSRDPLNRELGEQYKQEVLRWGGARDPWEMVSTLLDQPELAAGDAEAME",
    "response#2": "MRAAPNEEPRRKLYLAANSSTPEQIHVLETLLKKRAELAQLVGRDSFAHMTLDDKMAKKPEHVTNFLDALIDHTRPFARNALRTLAQRKQAHHNLPALPVIQAWDRDFYCPPDPPAPPIPLPPLTIGTVFMGLSRLFRHLYGVSLRPAQAASGEVWHPDVQKLEVVDEQQGIIGWIYADLFPRRGKASGAAHYTVRCSRRTDDDDEANDGMFEGTELQIQESQQFEAVKRHRLPNQEGVYQLPLVVLLTEFARPSLSKGAAVLEWHEVQTLFHEMGHAMHSMLGRTEYQNVSGTRCATDFVELPSILMEHFLNSPAVLSLFDADNTTSLRQIGNHHNDPCHAIDTYSQIMLAVVDQVYHSPSVLNSSFDSTNEFANLVNKRGLIPYVPGTSFQTQFGHLFGYGATYYSYLFDRAIASRVWSKVFSRDPLNRELGEQYKQEVLRWGGARDPWEMVSTLLDQPELAAGDAEAMG",
    "response#3": "MRAAPNEEPRRKLYLAANSSTPEQIHVLETLLKKRAELAQLVGRDSFAHMTLDDKMAKKPEHVTNFLDALIDHTRPFARNALRTLAQRKQAHHNLPALPVIQAWDRDFYCPPDPPAPPIPLPPLTIGTVFMGLSRLFRHLYGVSLRPAQAASGEVWHPDVQKLEVVDEQQGIIGWIYADLFPRRGKASGAAHYTVRCSRRTDDDDEANDGMFEGTELQIQESQQFEAVKRHRLPNQEGVYQLPLVVLLTEFARPSLSKGAAVLEWHEVQTLFHEMGHAMHSMLGRTEYQNVSGTRCATDFVELPSILMEHFLNSPAVLSLFDADNTTSLRQIGNHHNDPCHAIDTYSQIMLAVVDQVYHSPSVLNSSFDSTNEFANLVNKRGLIPYVPGTSFQTQFGHLFGYGATYYSYLFDRAIASRVWSKVFSRDPLNRELGEQYKQEVLRWGGARDPWEMVSTLLDQPELAAGDAEAMRVATEQTYGEVASVGVWVDSGSVYETDKNNGVAHFLEHMIFKGTAKRPTPQSIETEIENMGGSLNAFTSREHSAYYMKVLKDNVPNAVDILSDILQNSKFETSLIEQERDTILSENDYIQSKEDEVVFDQLHAAAFQGSALGRTILGA"
  },
  {
    "reference": "MAGARAALLPLLLHLGSLALAARGGEVSREQPRLADAISQQQAPSHSLVPGETHQQQWCPLEERLERLEAEVTDLRKQNRELQARVVQLESCECWGPGHTCPEGARWEPDACTACVCRDGTAHCGPQPNLPHCRGCSHNGQSYGHGETFSPDACTTCRCLAGTVQCQGPSCSELNCLESFIPPGECCPICRPGCEYEGQLHQEGSSFLSSSNPCLQCSCLRSLVRCVPVKCQPSPCLNPVPRLGHCCPVCQASGCTEGNSHRDHGQEWTTPGDPCRICQCLEGHIQCRQRECASLCPYPARPLPGTCCPVCDGCFLNGREHSSGEPVGSQDPCSSCRCTNGSVQCEPLPCPPAPCRYPGRIPGQCCPVCDGCKYQGHEYRSQETFTLQENGRCLRCVCQAGEVSCEEQDCPVTPCVRSASGPQLCSACVLNGEEFAEGIQWEPDDQPCTSCSCQDGVPVCRAVLCSPVPCQHPTQPPGACCPSCDSCTYHSLVYANGQNFTDVDSPCQTCYCEDGTVRCSLINCPFTTCAKPQNGPGQCCPKCPDCILEAQVFVDGERFPHPRDPCQECWCQEGQAHCQLRACPSAPCVHPLPGTCCKNDCTGCAFGGKEYPNGADFPHPTDPCRLCRCLSGNVQCLARRCPPLSCPQPVLTPGDCCPQCPDAPADCPQSGNMVPVRHQEHFFQPGDPCSRCLCLDGSVSCQRLTCPPAPCAHPRRDACCPSCDGCLYQGKEFASGERFPSPNVACHVCLCWEGSVKCEPRTCAPAQC",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Ensure the presence of a signal peptide within the protein.2. The designed protein should contain one or more TIL domains that are essential for its function.3. The protein must exhibit the following characteristics: In contrast, it inhibits both the activin-A and TGFB1-mediated signaling pathways..4. The designed protein localizes to the extracellular space. The designed protein sequence is ",
    "response#1": "CKKHELYVSFRDLGWQDWIIAPEGYAAYYCEGECAFPLNSYMNATNHAIVQTLVHFINPNTVPKPCCAPTQLHAISVLYFDDSSNVILKKYRNMVVRACGCH",
    "response#2": "MNLIGMLGSGKYGTVMKGLLHDQEVAVKIYPEEHHQYYVNERNIYALPLMECPALLSYFGYDERCTMDGRMEYQLVLSLAPLGCLQDWLIANTLTFSECCGMLRSITRGISHLHTELRLGDQHKPCVAHRDINTRNVLVQADLSCCIADFGFALKVFGSKYEYKGEVAMAETKSINEVGTLRYMAPELLEGAVNLRDCETSLKQMDVYALGLVLWEVATRCSDFYAPGQATPPYKAPYEQEVGSHPSFDQMQALVVRHKARPLFPTGWGGGAAAKVVRDTCEDCWDHDADARLTSLCAEERMQEMSTR",
    "response#3": "DSSRMPSVGDYNTSEQKQACKKHELYVSFRDLGWQDWIIAPEGYAAFYCDGECSFPLNAHMNATNHAIVQTLVHLMFPDHVPKPCCAPTKLNAISVLYFDDSSNVILKKYRNMVVRSCGCS"
  },
  {
    "reference": "MLRLGSHVSMKAPDMLLGSANEAASYGANTFMIYTGAPQNTRRKPIDELKIDEAQPIIEAHDLRQIVVHAPYIINLGNTKKPGYFEFATDFLYQEIQRADAVGATQLTLHPGAHIGAGANVAITQIIKGLNAVIRPEQHVQIALETMAGKGTEVGRTFEELAQMIDGVTYNEKLSVTFDTCHTSDAGYAIKDDFDGVLNEFDHVIGLDRLKVIHLNDSKNPQGAHKDRHTNIGMGTIGFDTLNKVAHHPQLPDISKILETPYVGEDKKHQLPPYKYEIAMLRAGQFNPHLIEDIEQQR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be designed to have high stability and activity towards Endonucleolytic cleavage to 5'-phosphooligonucleotide end-   products.. The designed protein sequence is ",
    "response#1": "LFDAARKKRLPFMPKVIGVVTSPTGAVIRDILHRISDRFPVHVLVWPVKVQGEGAGDEVANAIRGFNALEPGGAIARPDVLIVARGGGSLEDLWSFNDEIVVRAAAESRIPLISAVGHETDWTLIDYAADVRAPTPTGAAEMAVPVKAELEAQATALAARLQGCINRQMDQRRQSVRALMRALPSLDQLLALPRRRFDEAAAGLGRGLELNTINKRRGFERVASHLRPDVLSNRIAERRQLLNDRMARAERTVERLLDRSKSRIDRAEAILASS",
    "response#2": "LFDAARKKRLPFMPKVIGVVTSPTGAVIRDILHRISDRFPVHVLVWPVKVQGEGAGDEVANAIRGFNALEPGGAIARPDVLIVARGGGSLEDLWSFNDEIVVRAAAESRIPLISAVGHETDWTLIDYAADVRAPTPTGAAEMAVPVKAELEAQATALAARLQGCINRQMDQRRQSVRALMRALPSLDQLLALPRRRFDEAAAGLGRGLELNTINKRRGFERVASHLRPDVLSNRIAERRQLLNDRMARAERTVERLLDRSKSRIDRAEAILASG",
    "response#3": "LFDAARKKRLPFMPKVIGVVTSPTGAVIRDILHRISDRFPVHVLVWPVKVQGEGAGDEVANAIRGFNALEPGGAIARPDVLIVARGGGSLEDLWSFNDEIVVRAAAESRIPLISAVGHETDWTLIDYAADVRAPTPTGAAEMAVPVKAELEAQATALAARLQGCINRQMDQRRQSVRALMRALPSLDQLLALPRRRFDEAAAGLGRGLELNTINKRRGFERVASHLRPDVLSNRIAERRQLLNDRMARAERTVERLLDRSKSRIDRAEAILASR"
  },
  {
    "reference": "MSTVKARKPESWKIFDDIAPTYDFLNHFLSLGIDVLWRKNFLRNLPNKQDLVAYDLATGTGDVALALAKDSKIKTVTGIDLSVGMLEYGKQKVKKNKLENKIFMEEGDGVTIPKDDNSADVITVAFGIRNFPDPQTSLINMVRVLRNGGRAMIMEFSLPKNFIIRFFYLLYFRHILPAIGNAFSKNKDAYTYLNESVEDFPYGKDFTDMMVVAGMKNVRIVPLSFGIASLYIGDVEK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2).2. The protein design must be able to perform S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity, S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity, S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity in order to contribute to ubiquinone biosynthetic process.3. The designed protein should interact with enzymes and substrates within the menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2, Quinol/quinone metabolism, menaquinone biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MDKIESAIEDLKAGKMIIVVDDEDRENEGDFITAAEMITPEAINFMAKYGRGLICLPMVDEKLDELEITPMVSSNTSSFGTAFTVSIEAREGTTTGISAHDRAATVKALIDPKTKPTDLARPGHIFPLRSRKGGVLVRAGHTEAAIDLARIAGLYPAGVICEIMNEDGTMARVPQLELLAKKFNLKIITIADLIKYRRRTEKLVRCIEV",
    "response#2": "MDFFNTINEAIEDIKNNKLIIVVDDEDRENEGDFICAAEKVTPEIINFMITHGRGLVCVPISEERCEELSLDMMVKNNTSIHETNFTVSVDLIGHGCTTGISAHDRAKTIKALIDENTKPEDLGRPGHIFPLKAKQGGVLRRAGHTEAAIDLAKMAGLYPAGVLIEIMNDDGTMARLPELLKIGKKFNLKIITIKDLISYRLKKESLIKKEVIDCVYDLMNDVMSLGTHRLPIEIKAYQFKTKKDNKMGHYNEKYLKTKKDKMGHYNEKYLKTKKDKMGHYNEKYLKTKKDKMGHYNESYLKTKKDNMGHYNENYLKTKKDKMGHYNESYLKTKKDKMGHYNESYLKTKKDKMGHYNENYLKTKKEKM",
    "response#3": "MKIVDLIGDRLEQIAAGKGDLDAYDFLNRLLSLRRDVSVEPTIFFDCLNDESRFDKAVAAKSAACIVAERKFPLPKPTIIVPDVRKAMSLMSAKFFGHPSRKMKVIGC"
  },
  {
    "reference": "MHYLNISFTYKNTDISVREKLAFDSDEKKDQILRLLKSSKNIIECMVLSTCNRVEVLAYTNDIKSAMTHIIRCLTVYSGVFEDELFERADIYEDSGAVHHLFAVASSLDSLVVGETQIVGQLKNAFKFAFDNASSGEHISRLVHYACKCAARVRNETQISKNPISVSSVAVAKAKEIFGTLENKTAVVIGAGEMGELAAKHLITSGANVIIINRSSDHVEELVENLGDKASWDSILKLKEYINKYDLIFSSTSAPHAIITGELIEPQEFRRYFFDIAVPRDIDLVNTDKISVYSVDSLEEMVRRNLALREEQAQTAYSIVGQSTNEFLKFLKDNISIPLIKTIRKKAEICAEAELEKALKKGYLKHSDKEEAAKLIHQVFKAFLHSPTINLKSLASKNDAEQIAGGIKFLFDIKEENLENLTKDIDEI",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be designed to catalyze the following reaction (S)-4-amino-5-oxopentanoate + NADP(+) + tRNA(Glu) = H(+) + L-   glutamyl-tRNA(Glu) + NADPH.2. Ideal protein should have a binding affinity for key enzymes in the Porphyrin-containing compound metabolism, 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2, protoporphyrin-IX biosynthesis pathway to enhance the overall efficiency of the pathway.3. The protein must be able to glutamyl-tRNA reductase activity.4. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).. The designed protein sequence is ",
    "response#1": "TMNIREILVIGNGEMGRLMTNVLIENGYRATMTLRQYRYHANVIPLNANTVDYSNRYEKMKDCDVVISATLSPHYTVEMENLKKIDKIPKLFIDLAVPRDIDPSIKNLPNIELYDVDGIKLAQDFLDNELGKKIFDDFKGKQVLILGAGKMGELTAKHLHSNGAEQVTVINRTREKAAELAKRFLGVDRPYNELTEAIVEADILISSTGATGYVVTSDMVSHALKKRKGRPLFMVDIAVPRDLDPALASHDDVYLYDIDDLQNALNIFEKERIKDKKALVIGLGEMAQLVIKHLLNKQFEVLILGRNAAKFEDFVKELEEPKKVSFQNIENLNAYINEYELLFCATSSPHFIVQNRMLKETIFRRFWFDLAVPRNIEKPVLDNIFLYSVDDLEELLNLEARRIIEIEMASLMNKIKDVMIDEIFADFYKFAVVVQKMEIEKFSRMHPEIPASDVEAFAHSMINKVMNIPVMTLKSVARSQSNQDFSRIFSKFYKAYEIVGLATMEFYQWIQSLEVEPVIKDLRELARISAQKELQKALKKRYVPKEYESNIEKILHNAFNTFLHHPTIALKKNAQKEESDVLVGAIKNLFNEK",
    "response#2": "ELGKKIFDDFKGKQVLILGAGKMGELTAKHLHSNGAEQVTVINRTREKAAELAKRFLGVDRPYNELTEAIVEADILISSTGATGYVVTSDMVSHALKKRKGRPLFMVDIAVPRDLDPALASHDDVYLYDIDDLQNLFXKTXFXKFALNIFEKERIKDKKALVIGLGEMAQLVIKHLLNKQFEVLILGRNAAKFEDFVKELEEPKKVSFQNIENLNAYINEYELLFCATSSPHFIVQNRMLKETIFRRFWFDLAVPRNIEKPVLDNIFLYSVDDLEXKXKXSLFIFNAXALNIFEKERIKDKKALVIGLGEMAQLVIKHLLNKQFEVLILGRNAAKFEDFVKELEEPKKVSFQNIENLNAYINEYELLFCATSSPHFIVQNRMLKETIFRRFWFDLAVPRNIEKPVLDNIFLYSVDDLEXKXKLXDXFFXN",
    "response#3": "TMNIREILVIGNGEMGRLMTNVLIENGYRATMTLRQYRYHANVIPLNANTVDYSNRYEKMKDCDVVISATLSPHYTVEMENLKKIDKIPKLFIDLAVPRDIDPSIKNLPNIELYDVDGLRLAKQHIEAGAKDFELGKKIFDDFKGKQVLILGAGKMGELTAKHLHSNGAEQVTVINRTREKAAELAKRFLGVDRPYNELTEAIVEADILISSTGATGYVVTSDMVSHALKKRKGRPLFMVDIAVPRDLDPALASHDDVYLYDIDDLQNALNIFEKERIKDKKALVIGLGEMAQLVIKHLLNKQFEVLILGRNAAKFEDFVKELEEPKKVSFQNIENLNAYINEYELLFCATSSPHFIVQNRMLKETIFRRFWFDLAVPRNIEKPVLDNIFLYSVDDLEELAKRIFGDMRAHKAMLVGAGEMAELAAMHLLQAGIADILVANRTLVRGQELAKQFNGHAIPFEDMPRHLLDVDIIITSTGSQEPIIRARDIRAALKIRKNRPMFFIDIAVPRDIDPDVNGLDNVYLYDIDDLKEEAEGIVRAELELLLRAMKHREVEPLLAEIRGRMESLRQREAGKAIKKIENSKDPERVVEGLTRSIVDKIFHDIALKIRDAAERDDREFLRMCSELFEAEGIVRAELELLLRAMKHREVEPLLAEIRGRMESLRQREAGKAIKKIENSKDPERVVEGLTRSIVDKIFHDIALKIRDAAERDDREFLRMCSELFG"
  },
  {
    "reference": "MMTSVGTNRARGNWEQPQNQNQTQHKQRPQATAEQIRLAQMISDHNDADFEEKVKQLIDITGKNQDECVIALHDCNGDVNRAINVLLEGNPDTHSWEMVGKKKGVSGQKDGGQTESNEEGKENRDRDRDYSRRRGGPPRRGRGASRGREFRGQENGLDGTKSGGPSGRGTERGRRGRGRGRGGSGRRGGRFSAQGMGTFNPADYAEPANTDDNYGNSSGNTWNNTGHFEPDDGTSAWRTATEEWGTEDWNEDLSETKIFTASNVSSVPLPAENVTITAGQRIDLAVLLGKTPSTMENDSSNLDPSQAPSLAQPLVFSNSKQTAISQPASGNTFSHHSMVSMLGKGFGDVGEAKGGSTTGSQFLEQFKTAQALAQLAAQHSQSGSTTTSSWDMGSTTQSPSLVQYDLKNPSDSAVHSPFTKRQAFTPSSTMMEVFLQEKSPAVATSTAAPPPPSSPLPSKSTSAPQMSPGSSDNQSSSPQPAQQKLKQQKKKASLTSKIPALAVEMPGSADISGLNLQFGALQFGSEPVLSDYESTPTTSASSSQAPSSLYTSTASESSSTISSNQSQESGYQSGPIQSTTYTSQNNAQGPLYEQRSTQTRRYPSSISSSPQKDLTQAKNGFSSVQATQLQTTQSVEGATGSAVKSDSPSTSSIPPLNETVSAASLLTTTNQHSSSLGGLSHSEEIPNTTTTQHSSTLSTQQNTLSSSTSSGRTSTSTLLHTSVESEANLHSSSSTFSTTSSTVSAPPPVVSVSSSLNSGSSLGLSLGS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be optimized for stability and expression in cytoplasmic stress granule where it will participate in hematopoietic stem cell homeostasis.2. The protein must exhibit the following characteristics: Required for efficient formation of stress granules.. The designed protein sequence is ",
    "response#1": "ETLDVQMKGEFYSENHHNPPPFAPPVMEFPAAHDRMVYLGLSDYFFNTAGLVYQEAGVLKMTLRDDMIPKESKFRLTTKFFGTFLPEVAKKFPNMKIQIHVSASTPPHLSVQPTGLTFYPAVDVQAFAVLPNSSLASLFLIGMHTTGSMEVSAESNRLVGELKLDRLLLELKHSNIGPFPVELLQDIMNYIVPILVLPRVNEKLQKGFPLPTPARVQLYNVVLQPHQNFLLFGADVVI",
    "response#2": "ETLDVQMKGEFYSENHHNPPPFAPPVMEFPAAHDRMVYLGLSDYFFNTAGLVYQEAGVLKMTLRDDMIPKESKFRLTTKFFGTFLPEVAKKFPNMKIQIHVSASTPPHLSVQPTGLTFYPAVDVQAFAVLPNSSLASLFLIGMHTTGSMEVSAESNRLVGELKLDRLLLELKHSNIGPFPVELLQDIMNYIVPILVLPRVNEKLQKGFPLPTPARVQLYNVVLQPHQNFLLFGADVVL",
    "response#3": "ETLDVQMKGEFYSENHHNPPPFAPPVMEFPAAHDRMVYLGLSDYFFNTAGLVYQEAGVLKMTLRDDMIPKESKFRLTTKFFGTFLPEVAKKFPNMKIQIHVSASTPPHLSVQPTGLTFYPAVDVQAFAVLPNSSLASLFLIGMHTTGSMEVSAESNRLVGELKLDRLLLELKHSNIGPFPVELLQDIMNYIVPILVLPRVNEKLQKGFPLPTPARVQLYNVVLQPHQNFLLFGADVVL"
  },
  {
    "reference": "MAELTTIARPYAKAAFDFAVEKQAVDSWAEMLGFAALVSENETMRPLLAGSMASSALAKLFIDVCGEQLNEHGQNLIKVMAENGRLEVLPAVAQLFAEYRLEWAKEVEADVVSATELSDAQQQQIGVSLEKRLARKVKLNCSVDAGLIAGVIIKAGDLVIDGSVSGKLARLSDKLQS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein can be targeted to plasma membrane, proton-transporting ATP synthase complex, catalytic core F(1) and perform proton-transporting ATP synthase activity, rotational mechanism.2. The protein must exhibit the following characteristics: It either transmits conformational changes from CF(0) to CF(1) or is implicated in proton conduction.. The designed protein sequence is ",
    "response#1": "XG",
    "response#2": "MLLV",
    "response#3": "SCGVQ"
  },
  {
    "reference": "MGGDHGHSHGDEGGSFYVHLIAGAAAGFAEHCGMYPIDTIKTHIQAIKPGAMQTSSLQITKHIIQQHGITGLFRGLTAVAAGAAPSHAVHFSIYELLKFKFIGSDEDHHPIKVGIAGAIATMTSEAVASPMDVVKQRLQLQITDYKGLTDCTKRIWVKEGIRGFYSGYTTTLVMNVPYNIVYFASYESLKKIIQPWFNNKNPEERSYQLIDHLVAGGGAGMLAAAFTNPFDVVKTRLQTQSDFIASSTINSAKSIKRYGGMMDAMKTIWIEEGMDGYLRGMKPRMVFHSMSSAIVWSVYEYFKFILGE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Probably required for heme synthesis of hemoproteins and Fe-S cluster assembly .2. The protein can be targeted to mitochondrial inner membrane and perform ferrous iron transmembrane transporter activity.3. The protein should contain Helical; Name=1 transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "GLNSDSDKIPFHPYYTIKDLLGVFILLMVLMFLVLFFPDILGDPDNYTPANPLNTPAHIKPEWYFLFAYAILRSIPNKLGGVLALILSILILAAFPLLNSSKQQGLVFRPITQILYWFLVANLVILTWIGGQPVEYPFTIIGQIASISYFSIIVILXPMASTIENNIMKIV",
    "response#2": "LLKIINHSFIDLPTPSNISSWWNFGSLLGICLMIQILTGLFLAMHYTSDTATAFSSVTHICRDVNYGWLIRYLHANGASMFFICLFLHVGRGIYYGSFMLHETWNIGIVLFLTVMATAFVGYVLPWGQMSFWGATVITNLLSAIPYIGPTLVEWIWGGFSVDKATLTRFFAFHFILPFIITALVLVHLLFLHETGSN",
    "response#3": "LFILAFFTGGALTLYAARASEMQAKGLFSMVSRESALVMNNVLLAVAALVVFTGTVWPLIAELFWDRKLSVGAPFFEKAFTPFMVGLALLLPLGSMMPWKRASLGKLVRPLLPALVLTLAVLALVWVMATGRPMLALGAAGLGAWILFGALAEIWQRAGRTPGRILRLPRADWGKAFAHGGLGIVFAGVGLLMAGQVEDIRVAKAGDSFEVAGYTITLVSVEDVPGPNFTAKTATMEVRQGGKLVATLHPEKRIYPVQAMPTTEADIDNGFWRDVYLVIGDPQEGGGWAVRTYVKPFANWIWAGCLLMAFGGGLSLTDPLMKIINHSFIDLPTPSNISAWWNFGSLLGFSLMIQILTGLFLAMHYIPDTTVAFSSVAHICRDVNYGWLIRNLHANGASMFFACLFLHIGRGIYYGSYLYKETWNIGVILLLTVMATASMGYVLPWGQMSFWGATVITNLLSAIPYIGTTLVEWIWGGFSVDKATLTRFFALHFILPFMIAALTIVHLLFLHETGST"
  },
  {
    "reference": "MEYSVSQTSRTVVVLAAGEGKRMKSATPKVLHPLLGRTLVGHVLAAATAAGADRTIVVVGHKAEQVEAHLAEIAPEATAVLQAQQNGTGHAVRIALEAAPDLSGTVVVLNGDVPLLRAETVASLVAAHESQARGATVLAAEVPDPAGLGRIVRDASGNLERIVEERDASPRERAIREINAGIYAFDAAALREALGKLSTDNDQGEEYLTDVFGLLASVGRAVGVFVAPDAEETLGCNDRAELASLRALLRDRVNTAWMRSGVSILDPATTWIDVTVSLEPDAVVDQNSQLVGSTSVAAGAVVGPDTTLIDTSVGAGATVLRTHSIGAEVGPAASVGPFSFLRPGTRLARKSKVGGFVETKNAELGEGAKVPHLSYVGDASIGAQANIGAGTIFANYDGVRKSRTTVGEAAFVGSDTVLIAPVEVGPGAYVAAGSAIAQEVPSGSLGVTRAEQRNVEGWTSRRRPGTVSAAAAERFAAEGDGAAAE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should contain one or more MobA-like NTP transferase domains that are essential for its function.2. The designed protein should have a high specificity for Mg(2+), UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA binding, with minimal off-target effects.3. A protein that is capable of catalyzing reactions within the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway with high efficiency and specificity.4. A protein that contributes to cell wall organization, UDP-N-acetylglucosamine biosynthetic process, regulation of cell shape, peptidoglycan biosynthetic process by functioning in cytoplasm with glucosamine-1-phosphate N-acetyltransferase activity, magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity.5. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).6. Mg(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "VVILAAGKGTRMKSNLPKPLHEIGGQPMLTHVLNTARALNPTQLIVIYGHEGEKLKAHYHADQDITWVEQKTFLGTGDAMKYALPSILEDSNVLVLYADTPLIDQATLEKMLIKVDEQNVCWLTVKADQPGFGRVIREGDDFYAIAEAKDASEEQKKITEIYSGIMAVPAPQLKRWLQGLSNNNAQGEYYLTDIVAMAVADGVPVVAHQIDDEVQVEGVNNRLQLAELERAHQLRQARALMLA",
    "response#2": "VVILAAGKGTRMKSNLPKPLHEIGGQPMLTHVLNTARALNPTQLIVIYGHEGEKLKAHYHADQDITWVEQKTFLGTGDAMKYALPSILEDSNVLVLYADTPLIDQATLEKMLIKVDEQNVCWLTVKADQPELGLLLLKMSDPTGYGRILRDKDQAIVQIVEERDCNATQKEIAEINTGIMVVPTSQLKRWLARLDNSNSQKEYYLTDIVAMAVADGVAVKTAQPEQEFEVEGVNNRQQLATLERHWQNRVAEQLLEQCGVTLINPETVTIRGEIKLKGTDIIIEPNVTLEGVVSLGNNVHIGQGSVIQGAKLCDNVTILPYSMIEHSLLYDNVTIGPFARLRTNSILKADSKVGNFVETKNVTLGEGSKASHLTYLGDATIGRDVNIGAGTITCNYDGANKHQTIIEDNVFVGSNSSLVAPVEIKKGATIGAGSIISSTITEGALALTRATLRGWQRPVKIKK",
    "response#3": "IILAAGQGTRMNSDLPKVLHEVAGAPLLVHAMTAGATIAADRMIVVTGHGADAVAAAARAHDETVEIAHQAEQRGTGHAVAAALPLLEGFEGRVIVLFGDTPFIRTGTLEAMLAASSDVVVLGFEAADPTGYGRLLTDGSGAVSGIVEHKDASEAQRAIREVNTGILAAPTRHLKAWLARLRNDNAQGEYYLTDIVAMAVAEGVPVVSAQPAHAWETLGVNSKVQLAELERIHRRNQADALLVAGASLIAPETVWFSYDTQLGRDVLIEPNVFFGPGVRVADNVKIRANSHIEGAVIGEGCEVGPFARLRPGTVLEEKAKIGNFVETKKAHLGKGAKANHLTYLGDAEIGAKANIGAGTITCNYDGYFKYKTEIGEGAFIGSNSALIAPVKIGRDAIVAAGSAVSRDVADGDLRMVRAEGV"
  },
  {
    "reference": "MTEDTRINTVIEAIEEIRAGRVVIVVDDEDRENEGDFICAAELITPEIVNFMATHGRGLICVPLPEERCEEMELNMMVGHNTSLHETPFTVSVDLLSPETTTGISAKDRALTIKALVDPATKPYQLGRPGHIFPLKARTKGVLRRAGHTEAAVDLTRLAGLQPGGALVEIMNEDGSMARLPDLLKLSDRFGIKIISIRDLIRYRLGIESIVEMGEQVRLPTEYGDFDFIPFRQVSNGMEHAALVKGTWTKDEPVMVRVHSSCFTGDIFASKRCDCGPQLHKAMQMVEAEGKGAIIYLSQEGRGIGLINKIRTYKLQEQGRDTVEANIELGFREDERDYGIGANIMRGIGLGKVRLISNNPVKRAGLEGYGISIVETIPLEIPPNQFNSFYLETKAVKMGHTLACYKSPTKGEND",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..2. The protein contains novel GTP cyclohydrolase II domains that confer a unique function or activity.3. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+), GTP, Mg(2+) binding.4. A protein with 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, magnesium ion binding, GTP cyclohydrolase II activity, manganese ion binding, zinc ion binding is needed to enhance riboflavin biosynthetic process in a specific context.5. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.6. The designed protein is the optimization of the Nucleophile; for GTP cyclohydrolase activity, Proton acceptor; for GTP cyclohydrolase activity to achieve high catalytic efficiency for GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate. The designed protein sequence is ",
    "response#1": "TTRIPLQQHGDFNMTVFENDLDPAEHFALVKTPLFANQVPLVRIHSECITGDVFGSCKCDCGKQLESSLALIAAEGGVLVYLRQEGRGIGLANKLKAYALQEAGLDTVEANLQLGLPADDRDYAIAYQILKHLGIDTVRLITNNPSKIKALEEYGICVSERIPVIPPYDYLGPIKFPTKFGQFTLYAYESDVDPAPFVALVMGDVTNGEPVLTRMHSSCITGDVLQSMRCDCGDQLELALEKIGNEGRGCLVYIAHEGRGIGLINKLKAYELQDTGLDTVEANEALGFKADLRDYGLGAQVLQDLGIQKLRLMTNNPKKLAGAHGYGLEIVENVPY",
    "response#2": "TTRIPLQQHGDFNMTVFENDLDPAEHFALVKTPLFANQVPLVRIHSECITGDVFGSCKCDCGKQLESSLALIAAEGGVLVYLRQEGRGIGLANKLKAYALQEAGLDTVEANLQLGLPADDRDYAIAYQILKHLGIDTVRLITNNPSKIKALEEYGICVSERIPVEPGKSIPGRVSDINLPTDFGEFRAIAYTNDMDNNVHIALVKGNIRPEDAVLVRVHSECLTGDVFGSKRCDCGEQLHRAMERIDKERKGVILYMRQEGRGIGLANKLKAYELQDRGLDTVEANLKLGFSPDLRDYGIGAQILVDLGVRKMRLMTNNPKKIVGLEGYGLKVLERVREIEINLPTSFGDFNAIAYTNDLDGREHIALVKGDINPHEPTLVRVHSECLTGDVFGSHRCDCGPQLEAALKQIEEEGKGVLLYMRQEGRGIGLLNKLKAYKLQEEGYDTVEANHKLGFPADLRDYGLGAQILEDVGVRKMRLLTNNPRKIKGLHGYELELVERVPSA",
    "response#3": "MPRAVESRLPTAYGEFKLIAYDSFIDHHNHIAFIKGDVSTDEPVLVRVHSQCATGDTFASLRCDCGHQLASALTMIEKEGRGVLVYLMQEGRGIGLVNKLKAYNLQDEGLDTVEANEKLGFKADLRDYGIGAQILKDLGIRKMRLMTNNPKKIVGLEGYGLEIVERVPLQLPDERAVGRVSDINLPTDFGEFRAIAYTNDMDNNVHIALVKGNIRPEDAVLVRVHSECLTGDVFGSKRCDCGEQLHRAMERIDKERKGVILYMRQEGRGIGLANKLKAYELQDRGLDTVEANLKLGFSPDLRDYGIGAQILVDLGVRKMRLMTNNPKKIVGLEGYGLKVLERVPLRPPHGTVAEDARAVESRLPTAYGEFKLIAYDSFIDHHNHIAFIKGDVSTDEPVLVRVHSQCATGDTFASLRCDCGHQLASALTMIEKEGRGVLVYLMQEGRGIGLVNKLKAYNLQDEGLDTVEANEKLGFKADLRDYGIGAQILKDLGIRKMRLMTNNPKKIVGLEGYGLEIVERVPLRPPHGTVAEDAERIAEARLPTRHGEFRVLGYRSPLDGREHVALVYGDISGEEPVLVRMHSECLTGDVFGSMRCDCGEQLDAAMAQIAEEGRGAVVYLRQEGRGIGLHNKLRAYALQDRGKDTVEANEALGFKPDLRDYGIGAQILMDLGLERIRVLTNNPKKIVGLEGFPVEIVERVPLRPPHATVGGDRAVESRLPTAYGEFKLIAYDSFIDHHNHIAFIKGDVSTDEPVLVRVHSQCATGDTFASLRCDCGHQLASALTMIEKEGRGVLVYLMQEGRGIGLVNKLKAYNLQDEGLDTVEANEKLGFKADLRDYGIGAQILKDLGIRKMRLMTNNPKKIVGLEGYGLEIVERV"
  },
  {
    "reference": "MTTPEGDALAMRVEPVGLETEMQRSYLDYAMSVIVSRALPDVRDGLKPVHRRVLYAMYDGGYRPERGFYKCARVVGDVMGNYHPHGDSSIYDALVRLAQPWSMRMPLVDSNGNFGSPGNDPAAAMRYTECKMAPLSMEMVRDIDEETVDFTDNYDGRSQEPTVLPARFPNLLINGSAGIAVGMATNIPPHNLREVAAGAQWYLENYEASHEELLDALIERIKGPDFPTGALVVGRKGIEEAYRTGRGSITMRAVVEVEEIQNRQCLVVTELPYQTNPDNLAQKIADLVKDGKVGGIADVRDETSSRTGQRLVIVLKRDAVAKVVLNNLYKHTDLQSNFGANMLALVDGVPRTLSLDAFIRHWVNHQIEVIVRRTRFRLRKAEERAHILRGLLKALDAIDEVIALIRRSDTVEIARGGLMDLLEIDEIQANAILEMQLRRLAALERQKIVREHDELQAKITEYNEILASPVRQRGIVSEELTALVEKYGDDRKTKLIPYEGDMSIEDLIAEEDIVVTVTRGGYIKRTKTDDYRAQKRGGKGVRGTKLKEDDIVNHFFVSTTHHWLLFFTNKGRVYRAKAYELPDAGRDARGQHVANLLAFQPDETIAQIRAIRDYEAVPYLVLATKAGLVKKTPLKDYDSPRSGGVIAINLREQADGSDDELIGAELVSAEDDLLLISKKAQSIRFTASDDTLRPMGRATSGVKGMSFREGDELLSMNVVRAGTFVFTATDGGYAKRTSVDEYRVQGRGGLGIKAAKIVEDRGSLVGAL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings.2. The designed protein must be able to regulate DNA-templated DNA replication. The designed protein sequence is ",
    "response#1": "MGRSFSIVAPTGIGKTHFGLIMALYLARKGKKSYIIVPTSLLVRHLLDRANSFLEKLSAMKEPTPIVIGYYSGMQKKEMEETLNRIKNRDFSILITTDKFLYNKFELIKDIDFDFIFVDDVDSFLKSPKNIDKVVLLMGFSVEDIEIALAGEKKELAKATAPASARQNPGRVLIVSGATLRGKRTKRIKIFRSLFGFEPGTNLLGAFISEGFMSESRAGFNNVDRDYFNAVVAAYDAVVGGKRYVAQRTIASGSVLNELDIHDVSALKGTRLGVLCGQRSADKSVPEWLWQSPAAVKRVFLQALFEGDGSCSALPRNTIQVSYSTRSRQLAIDVQQMLLEFGL",
    "response#2": "MGRSFSIVAPTGIGKTHFGLIMALYLARKGKKSYIIVPTSLLVRHLLDRANSFLEKLSAMKEPTPIVIGYYSGMQKKEMEETLNRIKNRDFSILITTDKFLYNKFELIKDIDFDFIFVDDVDSFLKSPKNIDKVVLLMGFSVEDIEIALAGEKKELAKATAPASARQNPGRVLIVSGATLRGKRTKRIKIFRSLFGFEPGF",
    "response#3": "MGRSFSIVAPTGIGKTHFGLIMALYLARKGKKSYIIVPTSLLVRHLLDRANSFLEKLSAMKEPTPIVIGYYSGMQKKEMEETLNRIKNRDFSILITTDKFLYNKFELIKDIDFDFIFVDDVDSFLKSPKNIDKVVLLMGFSVEDIEIALAGEKKELAKATAPASARQNPGRVLIVSGATLRGKRTKRIKIFRSLFGFEPGLLGAFISEGFMSESRAGFNNVDRDYFNAVVAAYDAVVGGKRYVAQRTIASGSVLNELDIHDVSALKGTRLGVLCGQRSADKSVPEWLWQSPAAVKRVFLQALFEGDGSCSALPRNTIQVSYSTRSRQLAIDVQQMLLEFGD"
  },
  {
    "reference": "MPSPDAINRISYVSLVTNDPEKFDASFKFYNAMGFRLTKNFSRVSTNGAASGSHDPKLLLGVSHDSLREVWLESYPLQEVDSNGNLRPWQEMEVYDGDNSQKLTESTVVKLRLTNELLPLDKSLLQKQFVFFTTELKTVEARLEKINVKVGKIGDDILITEDPIGNIISFSNTKNELCKNTFTSAEEYVDLACEKILSTRKKVELEKEELLSEEAPKKAKKKIGVMTSGGDAPGMNPAVRAVVRAGIYYGCDVYAVYEGYEGLVKGGDLLKKMEWADVRSYMSLGGTSTNIGTARCMEFRQREGRLQGAYNMIINGIDALVVCGGDGSLTGADLFRSEWPSLVKELVDTGRLTKEQVAPYQHLTIVGLVGSIDNDMSGTDVTIGAFSSLERITEMVDYIGATAASHSRAFVVEVMGRHCGWLALMSGIATGADFIFIPERPPKAGEWKDQLKEVCSRHRSYGRRKTTVIVAEGAIDDELNPITSEEVKQVLVDLGLDTRNTILGHVQRGGTAVAYDRRLATLQGVEAVKAVLENTPETPSPMIGILKHKIVRTPLVDAVKQTKAVAEAIGNKDFDKAMSLRDNNFYDAYRYFRDISVYDDGSKQIADESKRLNVAIVHVGAASAGLNAATRALVLYSLSRGHNLYAVQDGFHGLANGNIKKLDWLQVEGWHNLGGSEIGTNRSLPSQNFGKVAFNLQKYNIQGLIIVGGFEAFASLHELSTQKKNYPIFEIPMVVVPATVSNNVPGSEYSLGSDTCLNQLVSYCDAVQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.2. The beta-D-fructose 2,6-bisphosphate, substrate, ATP binding site should be stable and able to withstand changes in environmental conditions.3. The designed protein must have at least one Phosphofructokinase N-terminal, Phosphofructokinase domain.4. The protein design should take into account the physiological conditions of the D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 3/4, Carbohydrate degradation, glycolysis pathway.5. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis..6. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-   bisphosphate + H(+) are properly positioned for optimal catalysis.7. The designed protein should be involved in regulation of intracellular pH by localizing to mitochondrion, 6-phosphofructokinase complex and exhibiting metal ion binding, proton-transporting ATPase activity, rotational mechanism, ATP binding, 6-phosphofructokinase activity. The designed protein sequence is ",
    "response#1": "MTNVIVGQSGGPTAAINASLAGVYRTAKDRGANKVYGMLHGIQGLLKEKYIDLSDHIKTELDAELLKRTPAAFLGSCRYKLPEIHENREVYDKIFEILDKLDIEVFIYIGGNDSMDTIKKLSDYAIIFGHAIRFIGCPKTIDNDLALTDHTPGYGSAAKYIGTSMKEIIRDSFCLEYEKGVVSIVEIMGRNAGWLTGAAALSKGEDCEGPDLIYLPELPFDLQLFGEKIKQLLQEKTSIVVAVSEGIRTEDGTYVCELGNSIDFVDAFGHKQLSGTASYLASYIAGEIGCKTRAIELSTLQRAASHAASRVDILEAYQVGGAAVAVASGNAKAAIAMEGNKVIGRVGVVLSGGQAAGGHNVIIGLFDALKTLNPDSCLIGFLNGPSGIIDGNVHELDEEFVSPYRNTGGFDLIGSGRTKIETEEQLKSSLETIQKFKLDGLVVIGGDDSNTNAVVLAEYLIKNGCTTKVVGVPKTIDGDLRNEYVEMSFGFDTACKTYSEMIGNLERDAISAKKYTHFVKLMGRSASHIALECALQTHPNYTFISEEVLEKKLTIKCITKELADLVEARSEKKKNYGVILIPEGLIEFIPEMKSLIEKLNDLLAGSEFASLDASASIDKVKKTLKEELLKTFEFLPEGIQKQLLLDRDPHGNVQVSHINTEKLFMETVAKELKQRDFKGSFSPVAHFFGYEGRAGFPSNFDATYCYALGYTAAASGIVYSCDGLVSSDARIGILTGGGDCPGLNPVIRAVVRKSLIEGYEIIGIKNGWKGLVENDTMKLDLTAVSGILPKGGTILGTSRTNPFKKPEDLKKVQDNFKKLGLYALVAVGGEDTLGAASKLIKNGIKNIVGVPKTIDNDLSCTDYTFGFDTALNIATECIDRLHTTAESHHRIMVVEVMGRHAGWIAIESGMAGGADVILIPEKPIDMDEVCDIIKRRHGRGKTFSIVVVAEGAQFKEKDMVLKEEKLDAFGHVRLGGIGEILAKEIESRTGYETRVSVLGHIQRGGTPTAFDRVLGTRMGVKAVEL",
    "response#2": "MTRVGVLTGGGDCPGLNAVIRATGRRVMVRGGEVVGVRDGWKGLVEGMFEPLGPREISGLLPRGGTIIGTTRTNPFKLEGGVEKVLDTFRNAELDALVAIGGEDTLGVAARLYEEHDFPVVGVPKTIDNDLSATDYTFGFDTAVSIATEAIDRLHTTAESHNRVMVVEVMGRHTGWIAVMSGIAGGADVILIPEQPISIDEACDDIRRRHERGKDFSIVVVSEGYELEGAQEGGEVDQFGHVRLSERGVGAALAQEIERRTGYETRVTVLGHVQRGGTPTARDRVLASRYGLKAADLARNGACGTMVALRGNRIEAVPLDEALGAVKTVPAELYETASVFFG",
    "response#3": "MNKIGVLTGGGDCPGLNAVIRAVVKKAEKGGDSVLGIKGGWAGLMNGLVEPLTDFSVSGILPKGGTILGTSRTNPFKNAEGSQKVMTNFKKYGLDAVIAIGGEDTLGVAKKFCDMGMPVVGVPKTIDNDLTGTDYTFGFDTAVNIVMEAIDRLHSTAEAHHRAMVVEVMGRQTGWIAACAGLAGGADCIVVPEKPFDIDEICKLIKKRHDRGKEFSIIVVAEGAKERGKNGFIAQTSKVDEFGHPQLGGVGAYIAAAIEKKIGIETRVTVLGHVQRGGTPTAYDRVLASRYGLKAVSAVKDGEWGKMAALRGDTIVEVPLAEALAAPKLIDPELYDMATGFFG"
  },
  {
    "reference": "MSRRASSCAHFAAVLFLLSVYSLGPAGDLGKRERLPRPAARRLRGLQGAPLRPTPGVQRPPGLGPAGARGTLQKSGRRRDSALGRGGVGWDHPYKARRPRGLRWSFAVAVCATMPPYTVVYFPVRGRCAALRMLLADQGQSWKEEVVSMETWQEGSLKASCLYGQLPKFQDGDLTLYQSNTILRHLGRTLGLYGKDQREAALVDMVNDGVEDLRCKYLSLIYTNYEAGKDDYVKALPGQLKPFETLLSQNQGGKTFIVGDQISFADYNLLDLLLIHEVLAPGCLDAFPLLSAYVARLSARPKLKAFLASPEHVNLPINGNGKQ",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein have GST N-terminal domains with specific sequence motifs that are crucial for its activity.2. The protein must contain a signal peptide for proper functionality.3. The designed protein should have S-nitrosoglutathione binding, glutathione transferase activity within mitochondrion, cytosol to regulate negative regulation of extrinsic apoptotic signaling pathway, negative regulation of ERK1 and ERK2 cascade, xenobiotic metabolic process, negative regulation of tumor necrosis factor production.4. The protein design should emphasize Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "GIEELAMVDVWTEVEAQQYYPAISPVVFECIIIPFIIPGGGAAPNQTVVDESLERLRGVLGIYEARLEKSRYLAGDSISFADLNHIPFTFYFMTTPYAKVFDEYPKVKAWWEMLMARPAVQRVCKHMPTEL",
    "response#2": "GIEELAMVDVWTEVEAQQYYPAISPVVFECIIIPFIIPGGGAAPNQTVVDESLERLRGVLGIYEARLEKSRYLAGDSISFADLNHIPFTFYFMTTPYAKVFDEYPKVKAWWEMLMARPAVQRVCKHMPTEC",
    "response#3": "CAWEEAQVNSVADQFKDYLNEVRPYFMVKMGFAEGDLDALAKDVFLPGFKKHYGFFANFLKSAGSGYLVGDSLTFVDLLVAQHTADLLAANAALLDEFPQFKAHQEKVHSNANIKKWLETRPVTPQ"
  },
  {
    "reference": "MAWFKRDNPPQAKGPAHRVKVPEGLWTKCVSCGETIYTKDIENNLNVCPKCNHHYRVSSKKRLELLLDEGSFTEFDAGVVSVDFLEFKDSKSYQDRIDQALAKGGSKDAIICGSGRIEGTPVQICVFDFSFMGGSMGSVVGEKITRGIERALSDRTPCIIVSASGGARMQESILSLMQMAKTSAALAKLREAGLPFVSILTDPTTGGVTASFAMLGDINMAEPKALIGFAGPRVIEQTIRQKLPQGFQRSEYLLDHGMVDVIVERSKMKSQLSSILTMLYRP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be optimized for zinc ion binding, carboxyl- or carbamoyltransferase activity activity in order to improve malonyl-CoA biosynthetic process.2. The protein should be designed to catalyze the following reaction acetyl-CoA + N(6)-carboxybiotinyl-L-lysyl-[protein] = malonyl-   CoA + N(6)-biotinyl-L-lysyl-[protein].3. The protein design should consider the endogenous proteins and enzymes present in the Lipid metabolism pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MQKTKLKDAVRTAVGKSKGRDLVICCMDFAFIGGSMGAVVGEKIARGIDHSIKNKIPFVMISKSGGARMMEAAYSLMQLAKTSAKLAQLAEAKIPYISLCTDPTTGGTTASYAMLGDINISEPGALIGFAGPRVVRDTTGKPVIRDPDRLPPVDRWETVRQARDIDRPTALDYLALAFDGFEELRGDRIGGDCAALVGGIARLGGRPVVVLGHQKGHTPAELARRNYGMASPRGYRKAARLMRLAEKLSLPVVTLVDTPGAHPGVEAEENGQAVAIAESIRLMTGLRVPVVAVVIGEGGSGGALALAAADEVLICERGTYSVISPEGCASILWSDPAMAPTAADALRVDARSLLRLGVVDGVVPEPAGGSQADHRAAADRLRSALAAALDEFKFL",
    "response#2": "MQKTKLKDAVRTAVGKSKGRDLVICCMDFAFIGGSMGAVVGEKIARGIDHSIKNKIPFVMISKSGGARMMEAAYSLMQLAKTSAKLAQLAEAKIPYISLCTDPTTGGTTASYAMLGDINISEPGALIGFAGPRVVRDTTGKPVIRDPDRLPPVDRWETVRQARDIDRPTALDYLALAFDGFEELRGDRIGGDCAALVGGIARLGGRPVVVLGHQKGHTPAELARRNYGMASPRGYRKAARLMRLAEKLSLPVVTLVDTPGAHPGVEAEENGQAVAIAESIRLMTGLRVPVVAVVIGEGGSGGALALAAADEVLICERGTYSVISPEGCASILWSDPAMAPTAADALRVDARSLLRLGVVDGVVPEPAGGSQADHRAAADRLRSALAAALDAVAPGRR",
    "response#3": "EVNNKLAKAWDRVQLSRMNDRPVGSDYIRELFTDFMEFHGDRYFADDKAIIGGVARFRGCPVTVIAQAKGTNTKENIERNFGMPSPEGYRKALRLMKQAEKFHRPVICFVDTPGAFCGLEAEERGQGEAIARNIYEMSGLKVPVLTIIIGEGGSGGALAMATSNEVWMLENSVYSILSPEGFASILWKDSSKAKDAAEVMRLTAEDLYQMGIVEQVLAEPSPYTIKNIKEVTGRLASKIEDFLIDLDEAASGPARCGSDCLDLIRHRKRPVLTDYLSLLFEDVIELHGDRYFGDDPALWCGLGRLDGQPVTIIGHRKGKTLDENARCNFGMPHPEGYRKAMRLMRQAEKFGRPVLCLIDTSGAYCGIGAEERGQGEAIAANLLGMAQLGIPVISLVTGEGGSGGALAIGVCDRLGMLSNALYSVISPRGFASLLWKDPSREKEAADVLRITASDLKRLGICDAIVAEPEPGAQADMAATATAMKVFFRQAVDAVARGELVGGSWLLCGGCRTMIYGRRFDRAGRVCADCGWHAPMTIDQRLDLLLDAGSRRDIEVTAIDGDPLEFVDSKPYPDRLRAARAATGLHDAAVAVRGTIDGNPAVVVVMDFRFLGGSLGAAVGEVFTRGAEVALRERTPLITVTASGGARMQEGAIALMQMAKTSQALGQLDEAGILTVSLITDPTFGGVAASFATLADVIIAEPGARLGFAGRRVIEQTIRQVLPDDFQTAEFLLTHGVVDIISPRAELRATLSRLLSVSSRREDGIPRQPARAR"
  },
  {
    "reference": "MSGEGRPQASVPQAGELRLGIAAIRWHAKITDRLLERALAAASEAGVAEPTVVRVPGSIELPVVCQQLAHQHDAVVALGVVIRGGTPHFEYVCDSVTAGLTRVSLDESTPVGNGVLTCDTEEQALARAGFEDSAEDKGFEATAAALETALVLRELRGFENGRGFL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein is the optimization of the Proton donor to achieve high catalytic efficiency for (2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-   ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2   H2O + phosphate.2. For general function, the protein need meet that Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2- butanone 4-phosphate3. A protein should be able to manipulate the activity of enzymes in the riboflavin biosynthesis, Cofactor biosynthesis pathway, either by activating or inhibiting their function.4. The protein should be able to modulate riboflavin biosynthetic process in a way that leads to a desirable outcome.5. The protein should have a high affinity for (2S)-2-hydroxy-3-oxobutyl phosphate. The designed protein sequence is ",
    "response#1": "MSQTHFGYESVPEAEKTGRVAQVFSSVAGKYEDIIANVTMGGLDRFWRRATVDIAGVRPGQRVLDIAGGTGDLAAKLSRLVGPDGQVILADINESMLKVGRDRLTDQGVSGNIQYVQADAESLPFPDNHFDLITIAFGLRNVTDKQKALASMLRVLKPGGRLLVLEFSKVAAPLEKAYDWYSFSVLPKLGKWVAGDEASYRYLAESIRVHPGQETLKQMMEQAGFERCDYHNLTGGIVAIHRGYKL",
    "response#2": "MSDAANRPENAAPTPAAAAIPAPPARPGESLAPDAPIGYYETVLGAFEQDMARRGYRFIGRINLVKPDQGVRERFQTVMDRHLRDGYEGLVVIGHDCPGLTADQVHDALGAAQRGAAVVAVEYGGARPLGDCAAANQRLAGAIGLLPGLGNPGGIGRLLRGAVENLMADMPRYTGTRDPSAMRPAGSIRTDADRTRARTAESAVLADGRVTDRPVRLRLLGSYAQAYPVVVGNTLDDALRAAGIRLGEFDFRKLPDREIACRDFACEDGLPAVPGERIDLDTPCAAGLELTVGGPVMARLRHLPAVAAVA",
    "response#3": "MKILGVNIDHIASLRNARGTVEPDPVKAALIAEQNGADGITFHLREDRRHIQDRDVFIFRQTVASKLNLEMAITDEMINIALEIKPEYVTLVPEKREEVTTEGGLDIVGQYTRIADIITKLHSMNIKTSFFIDPSNEQIEAAAKVNAHFVELHTGKYADAATEEEQLIELKRVKEAADYAHSLGLRVNAGHGLNYLNVDPIAAIEEIYELNIGHSIIANAVLVGLDKAVRDMKRLMLESKRI"
  },
  {
    "reference": "MKVMGTQRNCQRLWIWGILGFWVLLICSGNLWVTVYYGVPVWKEAKAPLFCASDAKAYEREVHNVWATHACVPTDPNPQEVVLENVTENFNMWKNDMVDQMHKDIISLWDQSLKPCVKLTPLCVTLNCTNVNVTNNVTTTEDHNTLNVTNKEEIMKNCSFNITTELRDKKKKEYALFYRLDIVPLGESNSSEYRLINCNTSTITQACPKVSFDPIPIHYCAPAGYAILKCNDKKFSGVGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVVIRSENLTNNAKTIIVHLNESVQITCTRLNNNTRKSIRIGPGQTFYATGGIIGDIRKAHCNIIRADWNRTLNNVTKKIRRKFNKTILFASSSGGDPEITTHSFNCGGEFFYCNTSDLFNTSITANTTKFNITLQCRIKQIINMWQEVGRAIYAPPIEGNITCNSSITGLLLTRDGGNINATNDTFRPGGGDMRDNWRSELYKYKVVEIKPVGIAPSTAKRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLRDQQLLGLWGCSDKLICPTAVPWNSSWSNKSLDDIWKNMTWMQWDREISNYTNTIYQILEDSQSQQEKNEKDLLALDSWKNLWTWFDISHWLWYIKIFIMIVGGLIGLRIICAVLSLVNRVRQGYSPLSFQTLIPNPRGPDRLGRIEEEGGEQDRDRSSRLDNGFLAIIWDDLRSLCLFSYHQLRDFILIVA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Include a signal peptide as an essential component of the protein.2. The designed protein should be involved in virion attachment to host cell, positive regulation of plasma membrane raft polarization, positive regulation of establishment of T cell polarity, viral protein processing, fusion of virus membrane with host plasma membrane by localizing to virion membrane, viral envelope, host cell endosome membrane, host cell plasma membrane and exhibiting structural molecule activity.3. For general function, the protein need meet that Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Transmembrane protein gp41: Acts as a class I viral fusion protein; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.4. The protein design should include a YXXL motif; contains endocytosis signal motif to ensure proper function.5. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "MRVMGTLRNCQQWWIWGILGFWMLMICSVMGNLWVTVYYGVPVWRDADTTLFCASDAKAHVTEVHNVWATHACVPTDPNPQEIYLQNVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTNVNSTNVNTTNTSNDNSTYSNKWEVKNCSFNVTTSIRDKVKKQYALFDTLDVVQIKEDSNTSYRLVSCNTSVITQACPKISFEPIPIHYCTPAGFAILKCNNKTFNGTGPCQNVSTVQCTHGIRPVVSTQLLLNGSLAEEGVIIRSENISDNTKTIIVQLSTAVDINCTRPSNNTRKSINIGPGRAFYATGDIIGDIRQAHCNLSRTQWNNTLGQIAKELRKHFNKTIVFNQSSGGDPEIVMHSFNCRGEFFYCDTAQLFNSTWDNNQLNSSETEGNDTITLPCRIKQIVNMWQEVGKAMYAPPIKGQLNCSSQITGLILVRDGGSSGNNTTEIFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKIICTTAVPWNSTWSNKSYEEIWNNMTWVEWEREISNYTSQIYEILTESQNQQDKNEKDLLELDKWASLWNWFDITKWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQIPTPNQRE",
    "response#2": "MRVMGIRKNYQHLWRWGIMLLGMLMICSATEKLWVTVYYGVPVWRDADTTLFCASDAKAHVTEVHNVWATHACVPTDPNPQEIYLQNVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTNVNSTNVNTTNTSNDNSTYSNKWEVKNCSFNVTTSIRDKVKKQYALFDTLDVVQIKEDSNTSYRLVSCNTSVITQACPKISFEPIPIHYCTPAGFAILKCNNKTFNGTGPCQNVSTVQCTHGIRPVVSTQLLLNGSLAEEGVIIRSENISDNTKTIIVQLSTAVDINCTRPSNNTRKSINIGPGRAFYATGDIIGDIRQAHCNLSRTQWNNTLGQIAKELRKHFNKTIVFNQSSGGDPEIVMHSFNCRGEFFYCDTAQLFNSTWDNNQLNSSETEGNDTITLPCRIKQIVNMWQEVGKAMYAPPIKGQLNCSSQITGLILVRDGGSSGNNTTEIFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRAVGIGALFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKIICTTAVPWNSTWSNKSYEEIWNNMTWVEWEREISNYTSQIYEILTESQNQQDKNEKDLLELDKWASLWNWFDITKWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQIPTHHQREPD",
    "response#3": "MRVKEIRKNYQHLWRWGTMLLGMLMICSAAEKLWVTVYYGVPVWRDADTTLFCASDAKAHVTEVHNVWATHACVPTDPNPQEIYLQNVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTNVNSTNVNTTNTDTDMRNCSFNITTMLQDKKQKKYALFYKMDLVGIDNEEGIGKNNTNHTYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCNDKQFNGTGPCKNVSSVQCTHGIRPVVSTQLLLNGSLAEGEIIIRSENLTDNTKNIIVQFNDTVNITCERPNNNTRQGIHIGPGKAYFTTNIIGDIRSAHCNISGNWNNTLKQVAKKLGKLLNKTTIIFEPAAGGDPEITTHSFNCGGEFFYCTTSGLFNSTWNSSTWTNGTWDNNTKTIPCRIKQIINMWQGVGKAMYAPPIEGRIRCSSNITGLILTVDRVDNDSDTNATFRPGGGNMKDNWRSELYKYKVVRIEPLGIAPTKAKRRVVEREKRAVGIGAMFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLRLTVWGIKQLQARVLAVEKYLKDQQLLGIWGCSGRLICTTNVPWNSSWSNKSYNEIWENMTWLQWDKEINNYTEIIYTLIEESQNQQEKNEQDLLALDKWASLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSIINRVRQGYSPLSFQTHLPSPRGPDRPEGIEEEGGE"
  },
  {
    "reference": "MIKITFPDGAVREFEIGTTTSEIAESISKSLAKKALAGKFNGQLIDTTRAITQDGSIEIVTPDHEDALDILRHSAAHLFAQAARRLFPDIHLGVGPAIQDGFYYDTDNEAGQISNEDLPRIEEEMKKIVKENFPSVREEVTKDEAREIFKNDPYKLELIEEHSEDAGGLTIYRQGEYVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGDSNNAMMQRIYGTAWFDKKDLKSYLKRLEEAKERDHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRIIERYITDKEVASGYQHVYTPPIASVDLYKTSGHWDHYREDMFPTMDMGDGESFVLRPMNCPHHIEVYKNHVHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVALDQVEDEFKRTLQLIIDVYEDFNLTDYSFRLSYRDPNDTHKYFDDDEMWEKSQTMLKAAMDDMGLDYYEAEGEAAFYGPKLDIQVKTALGNDETLSTIQLDFLLPERFELTYIGADGEEHRPVMIHRGIVSTMERFTAYLIETYKGAFPTWLAPHQVTVIPISNEAHIDYAWEVAKELRDHGIRADVDDRNEKMQYKIRQSQTKKVPYQLIVGDKEVENGTVNVRRYGSKQTHTESIAEFRENILADIKRKSRPDNAK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein contains novel TGS domains that confer a unique function or activity.2. The protein should be able to bind Zn(2+) ligand in a variety of conditions3. The designed protein should be involved in threonyl-tRNA aminoacylation by localizing to cytoplasm and exhibiting tRNA binding, ATP binding, metal ion binding.4. The protein must exhibit the following characteristics: Also edits incorrectly charged L-seryl-tRNA(Thr).; Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). The designed protein sequence is ",
    "response#1": "MPAITLPDGSVRDYPGPVTGTEIAASIGPGLAKAALAMEVDGKMMDLSRTLDADARLRFITAGDITLPDGSQRTFDHPVSVAEVAQSIGAGLAKATVAGKVDGKLVDACDLIDHDATLQIIAG",
    "response#2": "FATKSENRDFAIRPMNCPTCVQVYNTKLHSYRDLPIRMAEFGIVHRNEPSGSLHGLLRVRSFTQDDGHIFCTPEQVEEEVILMVQQCFEVYKDFGFNDFAVKIALRPENRIGDDETWDKSEQMLKNALDANNVSYELLPGEGAFYGPKIEFHLKDAIGRSWQCGTIQLDFSMPQRLGATYIDKNGEKQVPVMLHRAIVGSLERFIGMLIEHP",
    "response#3": "VEDEDKVLAVKPMNCPCHVQIFNHGLRSYRELPLRMAEFGACHRYEPSGALHGIMRVRAFAQDDAHIFCTEDQIADETVRFVEMLRRVYADFGFPEFAVKFADRPTPRSGSDEVWDKAEAALRSACETAGVEFTVNPGEGAFYGPKLEFVLRDAIGRDWQCGTLQVDFVLPERLDASYVTAESGRARPVMLHRAIVGSFERFIGILIEPTQAVIMNITDKQGEFALEVERTLNQGGFRAKCDLRNEKIGFKIREHTLLKVPYLLVIGDREVETHSVAVRTREGVDLGTMPVEQFREMITQVVVATITSDADDYAQRVAERLTSMGIRAEVDFRNEKINYKIREHSLAKVPVIAVVGRKEAENGEVALRRLGGEGQKVLSLEDAVRALTE"
  },
  {
    "reference": "MLLKDLTGEQVLAAPEFETEIAGLSADSRTVSRGFVFAALKGAKADGTRFIAAARDAGAGALLVGSDTPLAALGPDAGGCPVIVSDDPRRELALMASRFYERQPETLVAVTGTSGKTSVAVFTRQIFAHAGFEAASLGTIGTVTSRGAQYGSLTTPDPVSLHQTLARLADDGITHAALEASSHGLDQRRLDGLRLTAAAFTNLGRDHLDYHADVEDYLAAKMRLFDTLLEGDQPVVLEPSAPYAGRVLAVARELGRSVFTVGEGGDDLKLLDIRHDGFAQVLSIETIEGFFEVRLPLAGRFQVSNALVAAGLAICSGVPTSTALSALEDLQGAPGRLELVGETDDGALVFVDYAHKPDALDTALAALRPFTKGRLIAVFGAGGDRDPGKRPLMGQAAARQADIVIVTDDNPRSEDPAKIRAEVMDGAPEALEIGDRRDAIEAAIGMLQPGDVLCIAGKGHEPGQIVGDQVLPFSDHEVAREAIEAMAESALFDAAEDGGIDLGGDAGFDLDGALEQAFAENFERAEGDELSDHMPDEADDTEADGAEGGLHLELSEDDLLNDLFEEEDRSADDAPEHELSDEADALHVELAADETADEDVSGEDLLGEDLADEDLLGEELADEASVEEDVADDELPADDLMDDEPKAAAADEDDLLAEAEPESRGFPGISLSEEDLLGLSDPADEPDIAEDETAEDETAEDAIGERELEESLAEELVDDDEFDDEREPVETAPEVDDDIFGGADALTDEVEALETQSDEDAPADEPSA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should contain one or more Mur ligase central, Mur ligase N-terminal catalytic domains that are essential for its function.2. The protein must exhibit the following characteristics: Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein..3. The designed protein should have a binding site that is specific to the target meso-2,6-diaminoheptanedioate, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate ligand.4. The designed protein must be optimized for UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity. The designed protein sequence is ",
    "response#1": "MNLKDLLKGIDYELIKGNTDIDEISAVVFDSRKVVPGSLFVAVKGTLTDGHDYIEQAVKDGAVAVICEVPDKLDSNIKYICVENSRRVMSVISSNFYENPSKKLKLVGIVGTNGKTTTVFMIKHIFDEAGIKVGMIGTIKYNLGKNIIPVDRTTPESYDLHKILYLMKNEGCKYVVMEVSSHSLILHRTYGIDFDLAVFTNLTQDHLDFHKTFDNYYNAKFKLFEMIKNSIINIDDEYGKRLITEINKPILTYGIEKKADIYAENIKITMNGTEFYFTAKKNSFNVRIKLVGKFNIYNVLAAISASLCEGISLELIKRGIELLEGVPGRFDYIIAPNKIIGIVDYAHSPDAVQNILSTIHDIRKGNEKVITVIGCGGDRDKTKRPIMAKTACEWSDKVIFTSDNPRSEEPAQIIKEMEEGVDPAFKRHTLSIADRREAIKTACMLARPGDIVLIAGKGHETYQIFKDKTIHFDEREIVRKFINE",
    "response#2": "MISVESDSRKLTPGALFVALRGARFDAHDFLPQARAAGATALLAERGLAPAGTATIPVCVEADLRRTLARLAPGFFGRPACTAAVTGITGTNGKTTTAAAIYSILLDLGYKCFLCGTRGAFANDKRVDEKGLTTSSVIANLNYLKEATKLNCEYFIMEVSSHAIAQNRVEGLNFALKIFTNLSQDHLDYHKTMQEYARVKSSFFLDENTPKLINNDDEHIKFSYKNTQTYAIKKPATYSVVAYGLKNGINAVLRTPDGEVEIESDMVGEFNLYNLMAAFLALEEAGFPLAQFARALGRVKGVPGRLEQVGEVDGAICIVDYAHKPDALAHVLDALRPFVKGRLVCIVGCGGDRDRGKRPLMGRIASEKADIVIVTDDNPRSEDPAAIRAEVLKAAPGAREIGDRAEAIRTAVKELRQGDILVVAGKGHEPYQEGAGGIRPFSDLAEVAAALRGKP",
    "response#3": "MKVAGITADSRQVQPGDLFVAVSGTKANGTSYIAEALARGAVAVVAETGAKPDGATAPVLSLGNPRAFLARAAAAFYGDPARRLRMVGVTGTNGKTTVAFLVHHLLETVGVTGTNGKTSCALWTAQALNAAGQRCGVIGTLGAGWPDALQSTGFTTPDPVRLQALLATLREQGAQWCAIEASSIGLAEHRLAGCELHAAAFTNLTQDHLDYHGDMAAYARAKQALFAWPGLRHAVIDIDDPFGASTLLQDCRSRGLLVQRVALNAPADWRAENIEQSLAGQRFSLAHGHVRHAFDLPVLGVFNLHNLLVVAAVLDAVGVPFAVAGRLERVANDSGVLALVDYAHTGDALEQVLKTVSQLEHRRLYSVVGCGGDRDKSKRPVMGGVAVRYSDLAILTSDNPRTEDPLAILAQIREGALAAGSTELNEDQAGYEKGFIVIPDRRSAIEFAGRIAQSDDLILVAGKGHEPYQEVAGVRHPFDDREVAREALLQRWSRQGGG"
  },
  {
    "reference": "MRKKRKGSETEGWENLPDDLSCSTASRSSNFRSHFSLEGYARLKKRCKETEAVESVGSFKRRIAGVATAPPCGASSLVSSGRGLKRKIGCIDVSTQTGRKNKIDDDYVFGRNIGKGKFGSVRICKSRKNGTEFACKTLKKGEETVHREVEIMQHLSGHPRVVTLHAVYEESDCFHLVMELCSGGRLIDQMVKVGRYSEQRAANIFKDLMLVINYCHEMGVVHRDIKPENILLTAAGKIQLADFGLAMRIAKGQTLSGLAGSPAYVAPEVLSENYSEKVDVWSAGVLLYALLSGVLPFKGDSLDAIFEAIKNVKLDFNTGVWESVSKPARDLLARMLTREESARITADEVLRHPWILFYTDRTLKTMCIKSKHKSQAGSSTCLQNRSPTEKTDLNRADREKKIPSDSPTDSFSNTEEEEDESGVVDVLVVAIANVRISEPKRSRVCSPTNNPIEQQHSSNLTSTSTLCRAF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be involved in peptidyl-serine phosphorylation by localizing to nucleus and exhibiting calmodulin binding. The designed protein sequence is ",
    "response#1": "IETQEERQGKMLLHTEYSLLSLLHNQEGVVHHHGLFQDRACEIIEDLEANRMVRKMKKRICLVLDCLCAHDFSDKTADLINLQHYVIKEKRLSERETVVIFYDVVRVVEALHKKNIVHRDLKLGNMVLNKRTHRITITNFCLGKHLVSEDDLLKDQRGSPAYISPDVLSGRPYRGKPSDMWALGVVLFTMLYGQFPFYDSIPQELFRKIKAAEYTIPEDGRVSENTVCLIRKLLVLDPQQRLTASEEL",
    "response#2": "VTNHNSLGDGNFSVVKECMNIHTKDLYAMKLIKKQTVKNKIQLIQREFDLLRSISEKIRDMEKKNEHSLDIFEGHHHILQLFDYFETADNIVLITQLCQKGDLYEKIVENQCLDLETQVTSYCACLVSVLEFLHSQGIVHRDLKAENVLFRLRVNENEKNLQGEHHGDFKYDLLAHDLVLADFGLAAEYNTSKVNSLKEFVGTISYIAPEIVKCKGVGEMTPDQVGKLDKYGCPVDIWALGVLTYFMAFGYTPFDCTTDDETLECISKCDYYVDEQMMHDPKYEQFWNFVQCCFTIDPAVRRSAKNLKQHPFM",
    "response#3": "PTTKCFLRRFLRAAQVCHEAPVLHLEFLANYIAELSLLEYSLICYVPSLIAASSIFLAKFILKPTENPWNSTLSFYTQYKPSDLCNCAKGLHRLFLVGPGGNLRAVREKYSQHKYKFVAKC"
  },
  {
    "reference": "MPRSRDAVLSQVLTVAQMRAAEEDLIAAGTSVDALMRVAGRGAGEWVRRIAGGGSVTVLCGPGNNGGDGWVIAEYLREHGNAVQVVQAREPATDAARNARALYRGAVVSADSVTGGDVFVDCLFGSGLTRALPDEIYDMLCGLAALHRHRIAIDVPSGIESDSGAALNAGLPDWTLTVALGAWKHAHFAMPACAAMGATRLVDIGVAQVPGAARVLERLRLTSPAPDAHKYRRGMLGIVGGEMPGAAMLAATAALRAGAGYVKLAAQSAPAGVPPELVVTSDSAALLADARTSALLVGPGLGRSDGASRLLAAALHAGRPTVVDADALVLLRPAMLSGAPCVLTPHEGEMAALERAFGLWGKGLRRERALALAEVSGAVVVLKGPDTLIAAPGGEVVVAPRASSWLSVAGTGDVLAGTIASRLATNGQALRAAEEGLWLHGEAARLCGPAFTAGELADAVQAAFRAGSV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. We need a protein that can perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate with high efficiency.2. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.. The designed protein sequence is ",
    "response#1": "EEHEVSALMKHRPDFANKGTMGHALLVVGSYGMSGAAILASRACMRSGVGKLTVHTPARNYDIMQISVPEAVLQLDKDDYEFSDSVVCDDFDALGIGPGLGQSENTAVALIAQISRAKCPVVADADAINILASHQAWIQQLPKGIILTPHPKEFDRLMGSASASCYDRLMRARQLASRLQGYIILKGHYSALCLPDGQVLFNPTGNSGMATAGSGDVLTGIITGLLARGYNQADACTIGMYLHGLAGDLAMKDLGKESLVASDIISYLPKAFR",
    "response#2": "LSAKAAAALDRELMSTGAFSIDQLMELAGLSVSQAVARVHPTKQGRRVLVAVGPGNNGGDGLVAARHLFHYGYQPAIYYPKRPKNDLYQRLVKQCEDLEIPFVDDFFAALESTDHVVDAIFGFSFSGEVREPFPAVINAMAETKVPVTSVDAPSSWDINEGPPKSGVGSNFHPNVLVSLTAPKPLVKYFKGRHFVGGRFA",
    "response#3": "MQELDRQTIQEHGIPGLELMEHAGHACMEAIFHEFGYTGGKRAVIFAGRGNNGGDGYVIARLLLQSGWQVRVCILAERERIGGDAAINLEQLPPAAVSYCTVPGQLAGLLQEELSQTDVIVDALFGTGLNSEVAGINREAVELINAAGCPILAVDIPSGIHGTSGRVLGCAVRATVTVTFAAAKLGHVLYPGADHTGRLVVADIGIPAELVQ"
  },
  {
    "reference": "MEPVDPSLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFITKGLGISYGRKKRRQRRRPPZGSQTHQASLSKQPASQHRGDQTGPKESTKKVERETEADPVV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: This binding increases Tat's affinity for a hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA) and allows Tat/P-TEFb complex to bind cooperatively to TAR RNA; In the absence of Tat, the RNA Pol II generates short or non-processive transcripts that terminate at approximately 60 bp from the initiation site; The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive; In the cytoplasm, Tat is thought to act as a translational activator of HIV-1 mRNAs.; Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to promote processive transcription elongation by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts.2. Incorporating a Nuclear localization signal, RNA-binding (TAR), and protein transduction motif is critical for the successful design of the protein.3. The protein should be optimized for stability and expression in host cell nucleolus where it will participate in negative regulation of peptidyl-threonine phosphorylation, positive regulation of transcription elongation by RNA polymerase II, suppression by virus of host type I interferon-mediated signaling pathway. The designed protein sequence is ",
    "response#1": "MENRWQVMIVWQVDRMRIRTWNSLVKHHMYVSKKAKRWSYRHHYESTNPRVSSEVHIPLGEAKLVVTTYWGLHTGEREWHLGQGVSIEWRKDRYSTQVDPNLADQLIHLYYFDCFSESAIRNAILGHIVSPRCNYQAGHNKVGSLQYLALTALITPKRRKPPLPSVAKLTEDRWNKPQKTRGHRENHTMNGH",
    "response#2": "MEPVDPNLEPWNHPGSKPTTPCSNCYCKKCCWHCQLCFLKKGLGISYGRKKRKHRRGTPQSRKDHQNPLPEQPLSLSRGNSKSKESKKEVASKTETDP",
    "response#3": "MKVKGTQMNWPNLWKWGTLILGLVIICSASDNWXNXTXTXNSXNXTXGXEXEXEX"
  },
  {
    "reference": "MKFDKILIANRGEIALRILRACEEMGIATIAVHSTVDRNALHVQLADEAVCIGEPASAKSYLNIPNIIAAALTRNASAIHPGYGFLSENAKFAEICADHHIAFIGPTPEAIRLMGDKSTAKETMQKAGVPTVPGSEGLVETEQEGLELAKDIGYPVMIKATAGGGGRGMRLVRSPDEFVKLFLAAQGEAGAAFGNAGVYIEKFIERPRHIEFQILADNYGNVIHLGERDCSIQRRNQKLLEEAPSPALDSDLREKMGQAAVKAAQFINYTGAGTIEFLLDRSGQFYFMEMNTRIQVEHPVTEMVTGVDLLVEQIRIAQGERLRLTQDQVVLRGHAIECRINAEDPDHDFRPAPGRISGYLPPGGPGVRIDSHVYTDYQIPPYYDSLIGKLIVWGPDRATAINRMKRALRECAITGLPTTIGFHQRIMENPQFLQGNVSTSFVQEMNK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Ideal protein should have a binding affinity for key enzymes in the malonyl-CoA from acetyl-CoA: step 1/1, malonyl-CoA biosynthesis, Lipid metabolism pathway to enhance the overall efficiency of the pathway.2. The ATP, biotin, Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.3. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.4. The protein should be designed to have high stability and activity towards ATP + hydrogencarbonate + N(6)-biotinyl-L-lysyl-[protein] =   ADP + H(+) + N(6)-carboxybiotinyl-L-lysyl-[protein] + phosphate.5. The protein must exhibit the following characteristics: This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA..6. The designed protein have ATP-grasp, Biotin carboxylation domains with specific sequence motifs that are crucial for its activity.7. A protein with biotin carboxylase activity, metal ion binding, ATP binding functions is required to enable fatty acid biosynthetic process. The designed protein sequence is ",
    "response#1": "MQKSGKSEACRNATGKLGGSDAVISGMDFGFIGGSMGSVVGEKIARAADKSVELNAPLIVISQSGGARMMEGAFSLMQMAKTSARLTRLGERGIPFISLMTDPTMGGISASFAMLGDLNISEPKALIGFAGPRVIRDPKAYPGELPERDPWETVRRARDLDRPSTLDYARLAFDDFLELHGDRLAKDCPAIVGGPALLGGTPVMLVGTQKGHTVGELTARGFGMASPAGYRKAARLMRLAGKLGLPVVTLIDTPGAHPGLEAEENGQAFAIAENLRLMAGLPVPSVAVVTGEGGSGGALALAVADRVLMHADAVYSVISPEGCAAILWNDAAAAPRAAAALGVDSARLLRLGVVDAVIPEPGPGAPDDALATAGRLRDALLAVLAPGELPERDPWETVRRARDLDRPSTLDYARLAFDDFLELHGDRLAKDCPAIVGGPALLGGTPVMLVGTQKGHTVGELTARGFGMASPAGYRKAARLMRLAGKLGLPVVTLIDTPGAHPGLEAEENGQAFAIAENLRLMAGLPVPSVAVVTGEGGSGGALALAVADRVLMHADAVYSVISPEGCAAILWNDAAAAPRAAAALGVDSARLLRLGVVDAVIPEPGPGAPDDALATAGRLRDALLAVLARLRERRRRLDAPGELPERDPWETVRRARDLDRPSTLDYARLAFDDFLELHGDRLAKDCPAIVGGPALLGGTPVMLVGTQKGHTVGELTARGFGMASPAGYRKAARLMRLAGKLGLPVVTLIDTPGAHPGLEAEENGQAFAIAENLRLMAGLPVPSVAVVTGEGGSGGALALAVADRVLMHADAVYSVISPEGCAAILWNDAAAAPRAAAALGVDSARLLRLGVVDAVIPEPGPGAPDDALATAGRLRDALLAVLA",
    "response#2": "MQKSGKSEACRNATGKLGGSDAVISGMDFGFIGGSMGSVVGEKIARAADKSVELNAPLIVISQSGGARMMEGAFSLMQMAKTSARLTRLGERGIPFISLMTDPTMGGISASFAMLGDLNISEPKALIGFAGPRVIRDLPESCNSIPEEMYRSACVSTTEEAVASCQVVGYPAMIKASWGGGGKGIRKVHNDDEVRALFKQVQGEVPGSPIFIMKVASQSRHLEVQLLCDKHGNVAALHSRDCSVQRRHQKIIEEGPITVAPSETVKELEQAARRLAKCVHYVGAATVEYLYSMETGEYYFLELNPRLQVEHPVTEWIAEINLPAAQVVVGMGRSWGTIKELMKEREAKAKPFKVWDKVSAARQIKRLASVDYMDYIFDEFMELHGDRYFRDDPAIVGGIAYLDGQPVTVIGVHKGKDLEDCAKRNYGMPSPEGYRKALRLMKQAEKFNRPIITFVNTSGAYPGMEAEENGQGEAIARNLYEMSGIKVPILCLMIGEGGSGGALALSVGNEVWMMENATYSILSPEGFASILWKDGKRAKEAAEVMKITAHDLKELDVVDDIIPEFGGADEDALSSIGNYMKGNMKKFLEPGELPERDPWETVRRARDLDRPSTLDYARLAFDDFLELHGDRLAKDCPAIVGGPALLGGTPVMLVGTQKGHTVGELTARGFGMASPAGYRKAARLMRLAGKLGLPVVTLIDTPGAHPGLEAEENGQAFAIAENLRLMAGLPVPSVAVVTGEGGSGGALALAVADRVLMHADAVYSVISPEGCAAILWNDAAAAPRAAAALGVDSARLLRLGVVDAVIPEPGPGAPDDALATAGRLRDALLAVLA",
    "response#3": "MQKSGKSEACRNATGKLGGSDAVISGMDFGFIGGSMGSVVGEKIARAADKSVELNAPLIVISQSGGARMMEGAFSLMQMAKTSARLTRLGERGIPFISLMTDPTMGGISASFAMLGDLNISEPKALIGFAGPRVIRDPRNAVACVTTTEEAVASCQVVGYPAMIKASWGGGGKGIRKVHNDDEVRTLFKQVQGEVPGSPIFIMRLAAQSRHLEVQLLCDQYGNVAALHSRDCSVQRRHQKIIEEGPVTVAPRETVKELEQAARRLAKAVGYVGAATVEYLYSMETGEYYFLELNPRLQVEHPVTEWIAEVNLPAAQVAVGMGIPLEPESCNSIPEEMYRSACVSTTEEAVASCQVVGYPAMIKASWGGGGKGIRKVHNDDEVRALFKQVQGEVPGSPIFIMKVASQSRHLEVQLLCDKHGNVAALHSRDCSVQRRHQKIIEEGPITVAPSETVKELEQAARRLAKCVHYVGAATVEYLYSMETGEYYFLELNPRLQVEHPVTEWIAEINLPAAQVVVGMGLPIEVLSELDELRKKFKEKARKIYRDLSPWERVQVARHPKRPHTSDYIKYLIKDFEEVHGDTCYGDDKAVIAGFGYFRGKPVAVVGHEKGKDTKEKLERNFGMPHPEGYRKAIKVFKLAERYNIPVITFIDTPGAFPGIGAEERGQSRAIAESMLTMAFLKVPSVAVVIGEGGSGGALAFGVANRVCILENAYYSVISPEGCAAILWKDQSKVKEAAKALRLTAKDLKELGVVDCVIPEPYGAAHWSPRGTAMMVGMTLKKCLD"
  },
  {
    "reference": "MRNSKYVFVTGGVTSSLGKGIISASLAKLLQARGYTVTIQKFDPYINIDPGTMNPYEHGECYVTTDGAETDLDLGHYERFLNIETSQANNVTTGRIYQSVIENERKGEYLGKTVQVIPHITNEIKRRMLLLGESGKYDIIITEIGGTVGDIESLPFIESVRQLRWELGSEKVCIIHLTLIPYLKAARELKTKPTQHSVKDLQELGVQPDILVCRTEHKLNQELRNKIALFCNVSQNAVIESIDAETIYDVPLMMQKEKLDKVTLSKLKLPLKNEPELESWRDFLFKVKHPKNEVNIGLVGKYVELQDAYKSIIEAFIHAGAQNDCKVNVKTIHSDLLTTENVAEKLNNIDGVLVAPGFGNRGIEGKIEAIHYLRKNNIPFFGICLGMQMAVIEFARNELKLKNAHSTEMLENTQYPVIDLMEEQKNISSKGGTMRLGSYTCEIKKGTKVFSIYGKGKISERHRHRYELNNKFLKDLEKGGMIASGINKDLNLVEIIEIPNHRWFIGVQFHPELKSTVENPHPLFVKFVKACIDYSNEMSKKQGVALKN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen2. Ideal protein should have a binding affinity for key enzymes in the Pyrimidine metabolism pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MPDAYLSLLESLTHAGVANDAHVNVKWVNAEELAESGENIEALLGDADGILVPGGFGIRGIEGKVKAAEYARERGVPYLGVCLGMQVAVIEYARHKAGIKDANSSEFDPYAEHKVIDLMPEQLEVEGMGGTMRLGDWPMQLAAGTQVAEMYGVPQGGTVTERHRHRYEVNPQYVPQLQEAGLVISGVTPGVEGRGAGLVETVELPRNVHPYYVALQAHPEFKSRPMRPSPPFAGLVY",
    "response#2": "VEVALVGKYINLHDAYLSVSEAVKHGGFANNARVDLRWVASDTCETPEGAQKALEGVDAIIVPGGFGIRGIEGKLGALRWAREHKIPTLGLCLGLQCMVIEAARDLLGLTGASSTEMDPETPDPVVHTMDSQVDIVEGDGDLGGTMRLGAYPAVLQEGSVVAEAYGKTQVSERHRHRFEVNNDYRDQLESVGLHISGTSPDGNLVEFVELDRSMHPYYVATQAHPEFKSRPTKAHPLFAGLR",
    "response#3": "MPDAYLSLLESLTHAGVANDAHVNVKWVNAEELAESGENIEALLGDADGILVPGGFGIRGIEGKVKAAEYARERGVPYLGVCLGMQVAVIEYARHKAGIKDANSSEFDPYAEHKVIDLMPEQLEVEGMGGTMRLGDWPMQLAAGTQVAEMYGVPQGGTVTERHRHRYEVNPQYVPQLQEAGLVISGVTPGVEGRGAGLVETVELPRNVHPYYVALQAHPEFKSRPMRPSPPFAGLVY"
  },
  {
    "reference": "MIILENEANEPGFRIQQRAESETGKKGRFGGILKENPIKGFSSETLTRPLTGLFRKTGKSQRKTDDDDSQETIAAPPTNFPNMARISATIESVQINDEYAGYRDRNPETGSKLTLNEGAGVFGNAMHPLTRYFKVGDVDVSILGRSFPTEKTVDEEGPWTAQSLFNSLESRIKKERIDAKDQPSDSRSRNARETLISSKY",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.2. The protein must exhibit the following characteristics: As a component of IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein- coupled receptors (GPCRs), it is involved in ciliogenesis (Probable).3. The protein should be engineered to have a higher affinity for a specific molecule or receptor in intraciliary transport particle A, cilium that is critical for intraciliary retrograde transport. The designed protein sequence is ",
    "response#1": "WGYCSNTVVYAYTRPDRPEYCVVFWDTKNNEKYVKYVKSLISITTCGDFCILATKADENHPQFVLVLCNSIGTPLDPKYIDLVPLFVAMTKTHVIAASKEALYTWQYRVAKKLTALEINQITRSRKEGRERIYHVDDVPSGSVDGVFDYSKAIQGTRDPICAITASDKTLIVGRESGVIQRYSFPNVALIQKYSLDCRACQLSLNCNSSRLAIIDIAGVLTFFDLDTRVTDSTGQQVVGELLKLERKDVWDMKWAKDNPDLFAMMEKTRMYVFRNLDPEEPIQTSGYICNFEDLEIKSVLLDEILKNPEHPSKDYIMNFN",
    "response#2": "EQRWEWKNFQVTLTKRRTMQVRNVLNDAVDLLEFRDRVIKASLNHAHLVVSTSLQCYVFSTKNWNTPLIFDLKEGTVSLILQAERHFLLVDGGGIYLHSYEGRFISSPKFPGMRTDILNAQTVSLSNDTIAIKDKADEKIIFLFEASTGKPLGDGKLLSHKNEISEIALDQKGLTNDRKIAFIDKNRDLYITSVKRFGKEEQIIKLGTMVHTLAWCDTCNILCGIQDTRFTVWYYPNTIYVDRDILPKTLYERDASEYSKNPHIVSFVGNQVTIRRADGSLVHISISPR",
    "response#3": "EQRWEWKNFQVTLTKRRTMQVRNVLNDAVDLLEFRDRVIKASLNHAHLVVSTSLQCYVFSTKNWNTPLIFDLKEGTVSLILQAERHFLLVDGGGIYLHSYEGRFISSPKFPGMRTDILNAQTVSLSNDTIAIKDKADEKIIFLFEASTGKPLGDGKLLSHKNEISEIALDQKGLTNDRKIAFIDKNRDLYITSVKRFGKEEQIIKLGTMVHTLAWCDTCNILCGIQDTRFTVWYYPNTIYVDRDILPKTLYERDASEYSKNPHIVSFVGNQVTIRRADGSLVHISISPI"
  },
  {
    "reference": "MSDTTKVLVLYYSMYGHIDTLAKEIAAGVAEVDGVEVALKRVPEHMSAELLSTIHARTDFDTPIASVDELADYDGILIGTPTRFGNMAGQMRNFLDQTGGLWAKGKLVGKAGGAFTSTATGGGAETTLLSVYTNFLHHGMVVVGVPYGTPEMFDTSEARAGGPYGAATLAGGDGSRQPSDKERTIARFQGRHFAGVAKKLKG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. FMN is required for the proper folding and stability of the protein.2. The protein should have a high affinity for substrate, NAD(+), FMN.3. The designed protein must be optimized for NADH dehydrogenase (quinone) activity, NAD binding, FMN binding, NADPH dehydrogenase (quinone) activity, flavin adenine dinucleotide binding.4. The presence of Flavodoxin-like domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MKTLVILSSILGDRSHSKQLADHLIQRLRQHEPDGSIRVRDLAADPVPYFDGATAGALFTPADQRTAEQAAIVARSDALVAELFDADRIVFAVPVYNFGLPAQLKSYIDQIARAGVTFRYTAQGVPEGLLKNKQVVVLSARGGKAEGTPADTLTPYLAQVLGFVGLVDPVFISAEGMAMGELAAQDGLALARQRIDELLA",
    "response#2": "MNILLLDGGKAFGHSHGELNHTLHKKAKEVLTALGHNVKETVIDAGYDVEAEIEKFLWMDAVIWQMPSWWMHEPWTVKKYIDEVLTNGHGKLYHSDGRHSVNPTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKLNEFLGLTRLPTFLCNDVVKSPQVEQYLADYQAHLEAAL",
    "response#3": "MKTLVILSSILGDRSHSKQLADHLIQRLRQHEPDGSIRVRDLAADPVPYFDGATAGALFTPADQRTAEQAAIVARSDALVAELFDADRIVFAVPVYNFGLPAQLKSYIDQIARAGVTFRYTAQGVPEGLLKNKQVVVLSARGGKAEGTPADTLTPYLAQVLGFVGLVDPVFISAEGMAMGELAAQDGLALARQRIDELLA"
  },
  {
    "reference": "MSDRSPLHNARFAYIPKMPEILGSSLASIEAVPVSVVEPAHDKEALKKIFAHSYGKPAIRFSAGSNPSSNKTMTLGVLLSGGPAPGGHNVIAGLFDGLKKASPNSRLIGFKGGPGGLVDNKYQEIDAALVDSYRNTGGFDMIGSGRTKIESPEQFAKSLSVAREHSLDALVVVGGDDSNTNAALLAEYFASHGEAIAVVGVPKTIDGDLKNEWIEASFGFDTATKVYSELIGNICRDAPSGRKYWHFIRLMGRSASHIALECALQTRPNVALISEEIEARKTSLSAIVETIAASVAARAAGGENFGVVLIPEGLIEFVPEMKVLIANINELLARDAEAFKALPSLEAQVGFVAEQLPGESRALFLSLPADIRAQLCWDRDPHGNVQVSRIETEKLLVAMVKERLSAMAAQGTYKGKFDYQTHFMGYEGRCAFPTNFDADYCYSLGFTAFLLALNGLSGYIASIRGLAAPASEWVPGGIPLVSMMNIERRHGADKPVIRKALVELEGKAFKRFASQRDRWSKSSDYRLPGSIQYFGPPAVTDMPSILLQIEGK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein with metal ion binding, diphosphate-fructose-6-phosphate 1-phosphotransferase activity, 6-phosphofructokinase activity is needed to enhance fructose 6-phosphate metabolic process in a specific context.2. A protein that is capable of catalyzing reactions within the D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 3/4, Carbohydrate degradation pathway with high efficiency and specificity.3. For general function, the protein need meet that Consistently, PPi-PFK can replace the enzymes of both the forward (ATP- PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.4. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.5. The designed protein should have a high specificity for substrate, diphosphate binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "KIGVLTGGGDCPGLNAVIRGVVRKGVGLYGHSFVGFRDGWRGPLENDTMPLDIAAVRGILPRGGTILGSSRTNPFASADGPARVRETMRRTGVDALIAIGGEDTLGAAARLHAEGIPVIGVPKTIDNDLSATDYTFGFDTAVNIATEAIDRLHTTAESHHRVLVVEVMGRHAGWIALHSGMAAGANVILIPERPFDIDKVCAFVEQRFATSYAPIIVVAEGATPIAGTMMVKEGELDAFGHVRLQGISALLETEIRGRTGRETRATVLGHVQRGGTPTAFDRWLATRFGLHAVDAVHAGQFGVMVALRGTDIVRVPLEEATAELKTVPPERYAEAEVFFG",
    "response#2": "CLVAQSGGPTAVINASLAGVVRANQLNPVYDRVLGGLHGIEGTLAGKLFDLTDLSGRDLEILRQTPSSALGTCRYKLKRGNNADFLRLVEVMDDHDIDTLFYIGGNDSMDTVDALAEWAAENAPGKRFVGIPKTVDNDLVPVDHCPGFPSAAKVACQITHATRLDYDAYTRPEVFVLEVMGRDAGWLAASCCATGDVDLLVLPEVDFDKDAFLNEVRAKFDEQGECYIVVSEGAHYADGTYLSAGESANDGFAHAVLGGYAQAGGVTAVINASAAAVIEAAREHPQAIGTVYGARNGILGVLSETLVDTTPLTPAELAQLALTPGGAFGSCRFDLDPAEANPAQYDRLFEVFASYRIGAFLYNGGNGSMDIVARVAAAARARAYPLCCIGVPKTVDNDLEGTDCSPGFGSAAKYLATAMRESGLDLAAMSSRRGRVFVMEVMGRNAGWLAAATALAARNEDEPPHIVLLPEVAFDEEAFLARVKAVVERLGYCAVTVAEGIRRADGTLVMEQSHDALGHVQLGGAGQCVARLVSARFDYKHLWGTETGLEIPLNVGVILSGGQAPGGHNVISGLFDAMQAANPENKLYGFKGGPSGILKDDYRIIDAEYLSAFRNTGGFDIIGSGRTKLETVEQFETCARVCKKHEIGALIIIGGDDSNTNAAVLAEYFLEKNHGIQVLGCPKTIDGDLKNESIEISFGFDTATKTYAELVGNIARDANSAKKYWHFIRLMGRSASHISLEVALQTQPNFCLIGEEIGAKNQSLDDVVDELVRIIAARADDGNHFGVGLIPEGVVEFI",
    "response#3": "MATCIIGQSGGPTAVINASAQGVIQTALKADCITHVLGAAHGIKGVLEDKLYDMGQEDSGELDLLKFTPSSALGSCRYKLADPDVDDADYRRILEIFKKYDVRYFFYNGGNDSMDTCNKISKYMQKVGYECRIMGVPKTIDNDLNGTDHCPGFASAAKYIATSCAEVWQDAHVYDTGMVTIIEIMGRHAGWLAGSAALASLTGCGPDLVYLPETDFDMERFLADVKAVYDQSGNCLVAVSEGIHYADGTFVSEAKTSATDGFGHAQLGGLASHLASAVKEAFGVKNVGVILSGGQAPGGHNVIAGIFDGIKRLHKESKLYGFILGPAGLIDHQYKELTSEIIDEYRNTGGFDIIGSGRTKLETKEQFDKGLIILKKLDIKALVIIGGDDSNTNACVLAEYYKSINAGVQVLGCPKTIDGDLKNEQIETSFGFDTACKVYSELIGNIQRDCNSSRKYWHFIKVMGRSASHIALECALQTHPNVCIISEEVEEKDLSLDDIIEYIAGVIAKRAENGLNFGTVIIPEGLIEFIYAQAGGVTAVINASAAAVIEAAREHPQAIGTVYGARNGILGVLSETLVDTTPLTPAELAQLALTPGGAFGSCRFDLDPAEANPAQYDRLFEVFASYRIGAFLYNGGNGSMDIVARVAAAARARAYPLCCIGVPKTVDNDLEGTDCSPGFGSAAKYLATAMRESGLDLAAMSSRRGRVFVMEVMGRNAGWLAAATALAARNEDEPPHIVLLPEVAFDEEAFLARVKAVVERLGYCAVTVAEGIRRADGTLVMEQSHDALGHVQLGGAGQCVARLVSARFDYKH"
  },
  {
    "reference": "MTSKFAVILAAGQGTRMKSKLYKVLHPVCGKAMVEHVLTQIEQIKMDEIVTVIGHGAELVKERLVNRTDYAYQEEQLGTGHAVLQAEKLLKNKDGLTLIVCGDTPLFTAQTFTELFAYHEQKGAAATVLTAEAPNPFGYGRIVRNNLGIVEKIVEQKDASETEKKISEVNTGVYCFNNRDLFSALHLIKNDNVQGEYYLTDVMEILKKSGKVVAAYKMKNFDESMGVNDRIALAKANKLMRQRINEEHMRNGVTLVDPQTTYIDIDVKIGKDTVIEPGVVLKGETIIGEDCFIGAHSEICDTQIASNVKVTASFLEDAIMEKGSNIGPYSHLRPAAHVGENVHIGNFVEIKKAEIGKNTKVGHLTYVGDATLGEEINVGCGTVFVNYDGINKHHTNVGDYSFIGSGSNLIAPLNIADHAYVAAGSTITNNVDSHDMAIARGRQVNKKGYFDRYPVAKAAEISQKNTNKK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein should be able to manipulate the activity of enzymes in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, Nucleotide-sugar biosynthesis pathway, either by activating or inhibiting their function.2. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.3. The designed protein should be able to compete with endogenous proteins or inhibitors for acetyl-CoA, UDP-N-acetyl-alpha-D-glucosamine binding.4. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.5. The presence of MobA-like NTP transferase domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "IVLAAGEGTRMKSNQPKVLHQIAGLPLVCHVVKAVQGTGQSDVALVVGRGADAVQAAVEKISGSVSAYEQKERLGTAHAVLAAREAIVRGYDDLLIVFGDTPLIEAEALQAARAKLADGADIVVIGFEAADPGRYGRLVMQGGELVAIREEKDATDAERAITLCNSGVVAADAATLFRWLARVGNDNAKGEYYLTDVVALARADGLRAAAIRCDEAETLGVNTRAELAAAEAAFQAHARAAAMAGVTLVDPGSTTIDGAVRVQRDSVIGSDTVIYPGVFIRGEVKIGKNCSIGPYCVIRDGVTIGPDTEIGNFVELKNSRIGAGSKIKHLSYVGDAEIGNNCRVGPYARIRPGTRLDHDVHVGNFVEVKNAVFDGKSKGATLTAPDTVWFAHDTQLGRDVTVGPNVVFGPKVTVADGATIHAFSHLEGATVGAGAEVGPYARLRPGAVLGENSKVGNFVEMKKAILGAGAKANHLSYIGDADVGAGANIGAGTITCNYDGFLKYRTTIGAGAFVGSNSALVAPVAIGDGAIVAAGSVVTRDVAADALALVRPAGAKVPAGSLWFGLPAQKIKRK",
    "response#2": "AIILAAGQGTRMKSARHKVLHPVAGRPMLLHLLEAVEALGVDRKVVVVGSGREQVEAAVAGKGVSIAVQDRQLGTGHAVAQAHDALAGFAGDVLILYGDVPLVRTETMRAMVERLNRGDEPRAYGRVLRDGTGRVLAIVEERDADPAERGIREVNTGICLAPAAALADWLKRLDAKNAQGELYLTDIVARAVADGVPATLIAPETVYFSHDTVIGEDVLIEPNVVFGTGVRVGDGATVHAFTHLVDTVVSPGAAVGPFARSRGGTSVGPGSKIGNFVELKNAALAPGVKISHLSYIGDAVVGAAANIGAGTITCNYDGVAKHRTEIGAGAFIGSNSALVAPVTIGEGAFVASGSVITEDVPANALALARGRQVTIAGWKRPQKKP",
    "response#3": "MKAVILAAGEGTRMKSSRPKVLHPVCGVPMVLWSVQSAKELGAEPIVLVVGNGAEAVKEVVGDQVLYALQAQRLGTGHATLQARELLQGRAEVVLVLYGDMPNLRVETLARLYALHRERKPAIMTLLSAVMEDPTGYGRIIRNESGDVTRIVEQKDASPEELEIKEINTGVYVFKADLLRAALPQVQTDNSQKEYYLTDVLEIMRSEGHPIAAYRVSDPDEALGVNDRVQLAAASVILQRRINRELMLGGVSCADPDRVDIRGQVIAGQDVFIDVNNVFEGDVNMGDGVRIGPNCHIRNAIIGDNVEIKANSVIEDAEIGADSTIGPFARIRPDTRLAPGVHIGNFVEIKKSNVSSGSKINHLTYIGDADIGSGVNIGAGTITCNYDGVNKFRTTIADGVFVGSNSTLVAPVQLNDKSFIAAGSVITQATEAGKLTIARSRQRNIQGWVEKKGRGTAAAEAARRAKGGGEKK"
  },
  {
    "reference": "MSFLRRDKSKANFRDGSATGLEEPVSPTTHFSPNAPPPLDGNHGDHYHDPDSPRSSVVSLPQLIHNSATHHLKENYRGFHANKRPKGIANVPPLAQPIKPRFKKKSNSLLNKLIYSTKKEDDETATSGKESRSSSIISDEKRKSASSASSGSSRQKFRFSSFDSNLSTSSSSPPKDKKASISDTVSDSSTVTASMSNMPTISIDLNLDEMHDIIKSPETPAPTVGLPTQKAEKKASPTAIKNWQAPESWDVKAPIKKEEPHAPKIEEVAENDVAIDNVLEKKRLPVLYGTHQVPHVTNSKDIKSSHIIRVFKEDNTFTTILCPLETTTSELLAIVQKKFFLESTTNFQLSVCIGNCVKVLEDFEKPLKIQMGLLLLSGYTEEDKLRMLGREDLSFVCKFVVENIFLRSLTHDEEVLLSRNYVDVNISSLNLKNVPIIFHQHTYEIEKLNVANNPSIYLPLDFIQGCTSLAYVDFSHNGCSKFPNNLLEAPQLTHLNLEMNFLDEIPQRISCLSNLTNLKLSSNQLYSLPHSFSTLTNLKQLDLSSNYFDSYPEAVNKLTNLVELNFSYNDLSIIPESIANLINLQKLNLCTNKLSGTLPGYLSQLKALKRLDIRYNYISNVDVLGIIPNLEVAYASKNAISTFSDQMKCLRLLHFDRNPITELKFNTQMQMLSVLDLSRAKITAFPAEFVEKVPNIEKLVLDKNHLVSLPNELCQLSKLVSLSVHANNLQSLPANIGDLRFLKYLDLHSNNLKSLPDQIWDLCHLTSL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should contain one or more PPM-type phosphatase, Ras-associating, Guanylate cyclase domains that are essential for its function.2. Prioritize Polar residues, Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.3. For general function, the protein need meet that Plays essential roles in regulation of cellular metabolism by catalyzing the synthesis of a second messenger, cAMP.4. A protein that can perform adenylate cyclase activity, ATP binding, magnesium ion binding in mitochondrion, endoplasmic reticulum membrane, plasma membrane to support positive regulation of phenotypic switching, evasion of host immune response, positive regulation of apoptotic process, positive regulation of filamentous growth of a population of unicellular organisms in response to starvation, positive regulation of filamentous growth of a population of unicellular organisms. The designed protein sequence is ",
    "response#1": "MSGPRSASPPGCAATRGGPEHRAAWGEAEARANGHPHAAGGATRGCSKKPGGAVTPQLQQQQQQQQHEQQHEQQQHEQQQHVQQQQRLAKRWRGDDDPPLGGDDPLAGGFGFSFRSRSAWQERGGDDCRRGSRRRRRGAAGGGSSRAPPAGGGGGPAAAGGAEVRPRSVELGLDERRGRGRAEPEPEAEAGAPGGDRGARDGDGPAGPGACCRALLQIFRSKKFPSDKLERLYQRYFFRLNQSSLTMLMAVLVLVCLVMLAFHAARPPLRLPHLAVLAAAVGVILVMAVLCNRAAFHQDHMGLACYALIAVVLAVQVVGLLLPQPRSASEGIWWTVFFIYTIYTLLPVRMRAAVLSGVLLSALHLAIALRANAQDRFLLKQLVSNVLIFSCTNIVGVCTHYPAEVSQRQAFQETRECIQARLHSQRENQQQERLLLSVLPRHVAMEMKADINAKQEDMMFS",
    "response#2": "ALNASIPPLTNPMTPSMTFRNSNAGRISGVALVGVIIGGALALFLVVALGVVPYFFLHNTRDNNLAPKELTDPVTLIFTDIESSTALWAAHPELMPDAVATHHRLIRSLIGRYGCYEVKTVGDSFMIASKSPFAAVQLAQELQLCFLHHDWGTNAIDESYQQLEQQRAEEDAKYTPPTARLDLKVYSRLWNGLRVRVGIHTGLCDIRRDEVTKGYDYYGRTSNMAARTESVGNGGQVLMTTAAYMSLSAEEREQIDVTALARL",
    "response#3": "EQSQQQENLMLSILPKHVADEMLKDMKKDESQKDQQQFNTMYMYRHENVSILFADIVGFTQLSSACSAQELVKLLNELFARFDKLAAKYHQLRIKILGDCYYCICGLPDYREDHAVCSILMGLAMVEAISYVREKTKTGVDMRVGVHTGTVLGGVLGQKRWQYDVWSTDVTVANKMEAGGIPGRVHISQSTMDCLKGEFDVEC"
  },
  {
    "reference": "MTEAAFADAIVVAAGESRRMDGLDKLAADVGGRPLLAWTLEAIAASPRVARIVVVATSARVEAIRDAAWLPGKVLAVTPGGTRRQESVAAGLAALDRLPGDAAPDDRVILVHDGARPLVPVSLVSAVADAAAAHGAAIPVVSVAETLKRVEDGVVVATVDRQRMAVAQTPQGVRRGLLREAFAAHPADGAEAFTDEAALLEACRIAVHAIRGDPSNLKVTLPGDLRRVESALAGTRSSRVGLGSDRHPFGPGRPLAIGGIEIQGAPRLHGHSDGDVALHAVADALLGAAALGDLGRVFPADARTPRGIASGELVRDIVGRVARAGFRPVSVDLVIVGARPRLGDRLDEIGERVASLLGLPAAAVNVKASTGNLEGMEGAGRGISAQAIALLESIR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds; Catalyzes the conversion of 4-diphosphocytidyl-2- C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).2. The protein should be able to bind 4-CDP-2-C-methyl-D-erythritol 2-phosphate, a divalent metal cation ligand in a variety of conditions3. The designed protein should contain one or more 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domains that are essential for its function.4. The protein requires a divalent metal cation to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "TDDAAVAEWAGHRVHVFAGEDANMKVTTCKDLAIAEARLLGDLADVRTGQGYDVHAFGPGAHVWLGGLKIAHNHGLIGHSDADVLTHAITDALLGALADGDIGSHFPPSDPQWRGAASSIFLAAAAAKVRSRGGIIAHIDATLVCERPKLGPHRDAIRASIARIAEIPLDRVAIKATTSEGLGFTGREEGIASFAVATGVVSVVPTDDAAVAEWAGHRVHVFAGEDANMKVTTCKDLAIAEARLLGDLADVRTGQGYDVHAFGPGAHVWLGGLKIAHNHGLIGHSDADVLTHAITDALLGALADGDIGSHFPPSDPQWRGAASSIFLAAAAAKVRSRGGIIAHIDATLVCERPKLGPHRDAIRASIARIAEIPLDRVAIKATTSEGLGFTGREEGIASFAVATGV",
    "response#2": "TDDAAVAEWAGHRVHVFAGEDANMKVTTCKDLAIAEARLLGDLADVRTGQGYDVHAFGPGAHVWLGGLKIAHNHGLIGHSDADVLTHAITDALLGALADGDIGSHFPPSDPQWRGAASSIFLAAAAAKVRSRGGIIAHIDATLVCERPKLGPHRDAIRASIARIAEIPLDRVAIKATTSEGLGFTGREEGIASFAVATMR",
    "response#3": "TDDAAVAEWAGHRVHVFAGEDANMKVTTCKDLAIAEARLLGDLADVRTGQGYDVHAFGPGAHVWLGGLKIAHNHGLIGHSDADVLTHAITDALLGALADGDIGSHFPPSDPQWRGAASSIFLAAAAAKVRSRGGIIAHIDATLVCERPKLGPHRDAIRASIARIAEIPLDRVAIKATTSEGLGFTGREEGIASFAVATVRVGLGVDVHAFDAASPCRVGLLDFPGEPGLSGHSDGDAVAHAVCDALLSAGGLGDIGGRFGTSDPRYAGATGDVFVRGAVELLRSAGLAPTSVTVQVIGNRPRIGARRDEMQAALAAVVGAPVAVSGTTTDGLGLTGRGEGLAAIATAVLDA"
  },
  {
    "reference": "MAPKSKKQAATQSKSKKSKDLVDERFQAIVLTDSFETRFMPLTAVHPRCLLPLANVPLIEYTLEFLANAGVNEVYLMCSAHADQIQEYIENSKWMGDNSPFSVTTIMSIESRSVGDTMRDLDNRGLIAGDFLLVSGDVVTNMDFSKALQFHKQKKAQDKDHIATMVLNQASPLHRTRSQIDPAAFVLDKETNRCIFYQSIPPVSGKKTCISIDPELLEDFQGELQVRNDLIDCHVDICSPHVPQIFQENFDYQYLRSDFLKGVLTSDLLKKTIYAYISKDSSEYAARVESWSTYDAISQDILARWCYPLVPDSNLVEGNSYSYELNNIYKEDKIILAQSCKIGTSTSIGRNSSVGEGTQIKNSVIGRNCTIGKNVVIENSYIWDNAVIKDNSVLNRSIVAADAQIGNNVTLSPGSVIGFNVIIGDDKVIPHNVKIVETPIVTENEFGDFDDESNSEDENEYEDGNAVPVLAVKDVELVGETGKGFAYESEIESGDEDDEEFVGNGTYSGIIYQMKSLNVSDDSIASVSNKKVKKHSHRRRLSMNSMISDNGGAFESDEGEEEEDFGVEGLATVTRAIENNHDIDTALLELNTLRMSMNVTYHDVRSVTTQALVNKIVDFITTGTLTPQEAATKIFTKWGIMFKRQVFSPEEEVDLLNIVEEKSSVLDKAYNQIVLFLGVKSFYDMEVVEEENILKWWNDGENDEVRTLAAKFITWLQEADEEDSDEDDEDSE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Subunit of the guanine nucleotide exchange factor for eIF-2.2. The protein design should aim to mimic or enhance the function of natural proteins involved in cytoplasmic translational initiation, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "FEGIPSFAPELFRRVVLKDPLRSKALQRGLQQLSEEGATQLFKPLRNNDLILGAVGVLQFDVTAFRLKAEYNVDCVYEAVPVTTARWVSCSDPKKLEEFKRKVHDNLAEDGSGYLVYLATSRVNLSLL",
    "response#2": "EEEEAYQHILKALRTFDSEDAEDYTSEEAQQLALRALRSAISSPTRLSFEDIRALPAVHALSESHPVHYQLLQIFGEQDLDDYDDFREEHEGFIEKENLDNEVLYRKMRLLTFASLAAASMQTREISYNSITKALQIPSEDVEMWAIDVIRAGLVEGKLSQKKKVFLIHSVRR",
    "response#3": "RRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGSVKGRKATRHATSDWMEMEKQRGISVTTSVMQFQHRDRIFNLLDTPGHEDFSEDTYRTLTAVDSALMVIDSAKGVEERTIKLMEVCRLRDTPILTFINKLDREGREPVELLDEVERVLNIQCAPITWPIGMGKRFKGVYHLYEDAIHLFSASHGDRIVKGEVVEGLNSPKLDELLGDIADELRMEIELVRGASHEFDPDAYRAGRQTPVFFGSAINNFGILELLDAFAEYAPPHR"
  },
  {
    "reference": "SKLSALLFKHKEQLKAEILRKRALLDKELQIQVQEELKRDLKMKREREMAQAVQANAASVPTPSVPAPVPAPAPAAPPAPPRSPPPSTHSLPPAGHPTAPLPVTSQKRKREEEKDSKSKKKKMISTTSKEAKKDTRLYCICKTPYDESKYVPVPQLCS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be able to modulate anterior/posterior pattern specification in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "DDAVEKWDKTETDRFSGVKPAEEELDKDAVKQMFALGAATLGHVESLDVYQFSSEIAKARLSLFQKQADITKKHRGDANIRYAWVPAKKEVLSAVMMHGLGVGGAFIKKSMYGVGVHAANCPYFSARYCDIDDNGVRHMVLCRVIMGNMEPLRGDNTQYFTGGEEYDNGVDDVESPKHYLIWNMNMNTHIYPEFVVSFKLSIPNAEGNILPTTQSRHEC",
    "response#2": "WEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRII",
    "response#3": "DDAVEKWDKTETDRFSGVKPAEEELDKDAVKQMFALGAATLGHVESLDVYQFSSEIAKARLSLFQKQADITKKHRGDANIRYAWVPAKKEVLSAVMMHGLGVGGAFIKKSMYGVGVHAANCPYFSARYCDIDDNGVRHMVLCRVIMGNMEPLRGDNTQYFTGGEEYDNGVDDVESPKHYLIWNMNMNTHIYPEFVVSFKLSIPNAEGNILPTTQSRHEC"
  },
  {
    "reference": "MISAGDLRKGTTFELDGQVYTVIDFLHVKPGKGAAFVRTKLRNVISGGVTETTFNPTAKLQEAVIERKEMQYLYSDGELYYFMDQETFEQIPLNFEQVENAIKFLKENMFAIIKFYKGAAFSVEAPNFVELQIVECEPGIKGNTATNAMKPAKLETGAVVNVPLFVNEGETIRVDTRTGDYMERV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining translation elongation factor activity.2. For general function, the protein need meet that Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase.3. We require a protein that can specifically bind to the metabolites produced in the Protein biosynthesis pathway. The designed protein sequence is ",
    "response#1": "KLRNIGISAHIDSGKTTFTERVLYYTKRIKEIHEVRGRDNVGATMDFMDLEREKGITIQSAATYCSWDKDKNSYHFNLIDTPGHIDFTIEVERALRVLDGAVLVVCAVSGVQSQTVTVDRQMRRYNVPRVTFINKMDRMGANPFKAIEQLNSKLKLPAAAVQVPIGAESELKGVVDLLDMKAYYNKGDNGEIIESGPIPEELKSLAEEKRQVLIETLADVDEHMAEIFLEEKEPTIQEMKDAIRRATIARKFTPVLMGSALANTGVQHVLDAIVDYLPSQDEVKH",
    "response#2": "IMEPIMIVSVTSPNEFQGNVIGLLNKLQAVIQETDNGHDEFTLRAECSLSTMFGFASSLRASTQGKGEFSLEFSHYAPTAPHVQKC",
    "response#3": "IMEPIMIVSVTSPNEFQGNVIGLLNKLQAVIQETDNGHDEFTLRAECSLSTMFGFASSLRASTQGKGEFSLEFSHYAPTAPHVQKC"
  },
  {
    "reference": "METKVLMNITKFVANSIGHWRSQRSAHHLAFGHFEAVQSEIDIIALPHDDPAVIDLCKSYNIDPQTVVSPFRMTWEGQSDWDDSEIKGTCVLVPIPDPDSPHRGKLLRSRLCRNNRCRGDYYFTEHGTFVLVTAYERAAAEEKIWFVNPNVRCLCVSLIKTSVRFSELLLPHFLQKFARIFRIKLYMTIFKSYRYKLRACSLYINAIALALPCHFFINTGA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be able to modulate protein-phycocyanobilin linkage in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "HIQDETNRQKYVLLLDRGVTRNSSIQSLKKMIWETPLTVDTRWSIYDQLLQSDVRLISEQYIESFEQIMQVLNKMEFPRNKETEIEKLIDNSIKNKISGKQNISNDLLLMLQLKSISHQGLIPGDELLFHHFLTDPSTNQSLQHVWELVNLIQLTCFNDFTKEKYDIKIVEPRGVVSKYLSQDDSFKSEFNTSCLNSTTWWNILERVDHNLFMWIMDIIVVANSQCFKNYPINREKFKNKSWEYYKSKKVIVEYKVLASFALNVLLNYHFGFNDLISITTLL",
    "response#2": "SALDFKAMPTSPVPGIDSTSDLQFIRETTENLSSVYLQSSNRLSRSKLLERTDSAEVLPIEFKEASEKGMEDLILVSEDKLDVVSHSRPSWLPPKDPEEKKLHENQISKSMSIASFEQLDRNKC",
    "response#3": "HIQDETNRQKYVLLLDRGVTRNSSIQSLKKMIWETPLTVDTRWSIYDQLLQSDVRLISEQYIESFEQIMQVLNKMEFPRNKETEIEKLIDNSIKNKISGKQNISNDLLLMLQLKSISHQGLIPGDELLFHHFLTDPSTNQSLQHVWELVNLIQLTCFNDFTKEKYDIKIVEPRGVVSKYLSQDDSFKSEFNTSCLNSTTWWNILERVDHNLFMWIMDIIVVANSQCFKNYPINREKFKNKSWEYYKSKKVIVEYKVLASFALNVLLNYHFGFNDLISITTLI"
  },
  {
    "reference": "MIELVYKGLCPNCGGDICSERLGKGLMCKRCMQKEGDPCDILQEGDFLAICAVEQRSALFETFFKEKNGFSLRQIQKSWAKRFFLGHSFALLAPTGVGKTTFGLSLAAFLDQKSYLLFPTQLLVNQAVERLRQLGVEPLFYDSSFSKKKKDETKAKIRAGEFQILVTTTAFFYKNFESIPKAFGFVFIDDVDSILKSARHIDKVMLLLGFSQEDIDEAMCFIDLKIKGSLTYEEFEQWQAKMRFIRSHAKAKLIVSSATANPRSRRVGLFRELLGFEVSRPSITVRNVEDVYEEPKDLWQRSVELVNELGTGGLLFLPGNETKERLQEFLAFLEQKGIKAQSYEEFDEEAFRKGDAQVLVGFASYRNPLARGIDMPDVIRYALFVGVPKLEFTLDITKHTSLYFFLVSLLQFFKNHPNFEQFIQYLNYLKRVLRIPYEKLSQKAKAKVETIYHEITNLLDDKQIEKINKTPDVAIVKEDDHFKLVTADVTGYIQASGRTSRLYVGGLSKGLSYLLVDNQKAFVSLQKKVRWFNEEIVFKDAKDVDLKAILQIIDEDRQKIALAMQGELHEQKEFFTTSLVIVESPNKARTIANFYGKPMVRDLGGVRVYEVAKEGKILSIAASKGHVVDLTKQEGIYGILHEERFIPLFEPIDEDKKDIIGALRRLGVEVQDLFIATDPDVEGEKISYDLQLLVKPYNKKVQRAEFHEVTKRAFDEALSHPREFDEDLVKAQLVRRIADRWIGFSLSQMLQKRFQKSWLSAGRVQSAV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process2. The designed protein must have at least one Toprim domain.3. The protein design must be able to perform hydrolase activity in order to contribute to DNA topological change.4. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding. The designed protein sequence is ",
    "response#1": "VGGYPWNLQISWAYRIAQNQSFAMIAPTGVGKTTFGLITALFLSYKYNRKVYIVVPTSILVSHYYEKLLEYMNRAGIVVEVIAIHSKMSAKKRLELENGIKEGKFDILITTSNYLQRNFIDIFKPLIDKGIKINFIFADDVDAIMKGSKAIEYILLLMGFSEEDMQLGYKLAGLRLRLTRCEGVRERGYSEYCKEEGMPITEMFNEYQKKLAEKRANAGILVVSSATGKARGRRIKLFRELLGFIIGAGVEII",
    "response#2": "VGGYPWNLQISWAYRIAQNQSFAMIAPTGVGKTTFGLITALFLSYKYNRKVYIVVPTSILVSHYYEKLLEYMNRAGIVVEVIAIHSKMSAKKRLELENGIKEGKFDILITTSNYLQRNFIDIFKPLIDKGIKINFIFADDVDAIMKGSKAIEYILLLMGFSEEDMQLGYKLAGLRLRLTRCEGVRERGYSEYCKEEGMPITEMFNEYQKKLAEKRANAGILVVSSATGKARGRRIKLFRELLGFIIGAGVEIPS",
    "response#3": "RGENTILIAPTGIGKSTLLIVYAIYLARKGKRVIYVVPTKPLVHQVCKRIESYANNASLEIASAVVCYDSSYSKSRREALLDRIRSRDFKILVVTGNFVHKKINLLMGNDIDVLIVDDVDSLIKNEGGVYNLVKLAGLSDRSVELAKKRLNILWKILANRAYGRNVDELIKEFIRVDKELEAERALRRKCQLVVASATGRTRGIAGKLLRELFNVDPETIRRAEFHEVTRKAVSQALENLRSIDENLVKAQIVRRVEDRWIGFVLSQKLWSKFNNRNLSAGRAQTPVLGWVIERAEQHKEKKVVDVFRAEFHEVTRSAIIKALSELRDISENRVKAQIVRRIEDRWIGFELSQILQRVFKNRNLSAGRAQTPTLGWIIQRYGSDVFRAEFHEVTRSAIIKALSELRDISENRVKAQIVRRIEDRWIGFELSQILQRVFKNRNLSAGRAQTPTLGWIIQRYARRIFLGKSFALIAPTGMGKTVFGSIMALFLACEGNKKSYILVPTALLAKQVSERILKYSEKTGLNVRIAYYHSLLTGKEANSQLERIKSGNYDILVTTLNFLNMKFNLLKDIKFDFIFVDDVDSLLRASKNIDKVLILLGFDQKDLENALMIINLRGELSRELRKRSFSEVYRLYDELGAPMWSAQRAWALRALRGESFSIVAPTGMGKSTLGALLSVYLSHKKKKKSYIIVPTTPLVDMMFRKVSAFAEAFGVRAVYFHSKMSPSQRKEMKERLLSGDFDVLITTSRFLINNLDLLKNYEFGFVFVDDVDSVLKSPKNVDRILMVLGLQESDVKRLEEVDKELSRKAQVLTKLQDLQKRYQLLKEMRELEEELEDLKR"
  },
  {
    "reference": "MLNFAYQEHVRSYYFDSRNQDFQFPPLTQIEHADVCVIGAGFFGLSAALELAEKGKKVIVLEGARVGFGASGRSGGQAINGFEEGIDEYIKQVGEDKAHKLWNMSLETIDIIDERIEKYSIQCDWKKGYATLALNERRMDDLIEMEKESHKNFGYQNMQLWDKTKLKQHLGSDIYVGGLFDSNSGHLHPLNYCLGLAKACVDLGVQIFEQSPVVDMVEKNGCVEVKTAKSAVISQDVILATNAYIDVLPKSIHHGINRKILPVESFIIATEPLSQAVADSVINNGMSVCDNNLLLDYYRLSADNRLLFGSDSSSEKDMVAIMRKNMLCVFPQLENVKIDYGWAGPIDMTLNSTPHFGRISPHIYFAHGYSGHGVALTGLAGRIVAEAILGDDERLSIFEGLKVPSVYGGRIIKDLATKIGVQYYKFLDKYR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that localizes to cytoplasm and can perform oxidoreductase activity with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "VWNIYKQNRANWSTQLTELSRDQQLLLLQEANMTPAFKEWIHQLGWDGTQARAFEAFGETAGVINLAKVMPTMACAYTQLEPWLRLPKSAIYARYRLSDGQELVVVNLHAVNFTYGTQEYQQQLIALLDELRDFTGPVIVAGDFNSWSEARMALLSTQLASVGLQEVRFSPDNRTTFINGLPLDHVFYRGLQLEKAEAPISDASDC",
    "response#2": "ALTPSRLAVAFQAMGAMKLGLTIAIRYSHSRRQFGPKTKEEVKIIEHQTQTLRLMPHLATALALTFVSRYAGALLDEDVFQGKELVNSRSLQALVAGLKAYSTWENIRCLQDCRECTGGMGYMMENRISGLKCDTDVFATFEGDDVVMLQVVGRELL",
    "response#3": "VWNIYKQNRANWSTQLTELSRDQQLLLLQEANMTPAFKEWIHQLGWDGTQARAFEAFGETAGVINLAKVMPTMACAYTQLEPWLRLPKSAIYARYRLSDGQELVVVNLHAVNFTYGTQEYQQQLIALLDELRDFTGPVIVAGDFNSWSEARMALLSTQLASVGLQEVRFSPDNRTTFINGLPLDHVFYRGLQLEKAEAPISDASDV"
  },
  {
    "reference": "MKMKMKLASFGLAAGLAAQVFLPYNALASTEHVTWNQQFQTPQFISGDLLKVNGTSPEELVYQYVEKNENKFKFHENAKDTLQLKEKKNDNLGFTFMRFQQTYKGIPVFGAVVTSHVKDGTLTALSGTLIPNLDTKGSLKSGKKLSEKQARDIAEKDLVANVTKEVPEYEQGKDTEFVVYVNGDEASLAYVVNLNFLTPEPGNWLYIIDAVDGKILNKFNQLDAAKPGDVKSITGTSTVGVGRGVLGDQKNINTTYSTYYYLQDNTRGNGIFTYDAKYRTTLPGSLWADADNQFFASYDAPAVDAHYYAGVTYDYYKNVHNRLSYDGNNAAIRSSVHYSQGYNNAFWNGSQMVYGDGDGQTFIPLSGGIDVVAHELTHAVTDYTAGLIYQNESGAINEAISDIFGTLVEFYANKNPDWEIGEDVYTPGISGDSLRSMSDPAKYGDPDHYSKRYTGTQDNGGVHINSGIINKAAYLISQGGTHYGVSVVGIGRDKLGKIFYRALTQYLTPTSNFSQLRAAAVQSATDLYGSTSQEVASVKQAFDAVGVK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Incorporate a signal peptide in the protein design.2. The designed protein is the optimization of the Proton donor to achieve high catalytic efficiency for Preferential cleavage: Xaa-|-Leu > Xaa-|-Phe..3. The designed protein should be able to compete with endogenous proteins or inhibitors for Ca(2+), Zn(2+) binding.4. The protein design should prioritize the formation of strand-strand-strand-strand-strand-strand-helix-helix-turn-strand-strand-strand-strand-helix-helix elements.5. For general function, the protein need meet that Extracellular zinc metalloprotease.6. The ideal protein would be one that plays a critical role in proteolysis through metalloendopeptidase activity, metal ion binding within extracellular region. The designed protein sequence is ",
    "response#1": "QGSRWRVKNLTYKISKYPKRLKRNDVDAEIARAFAVWSEDTDLTFTRKTSGPVHIEIKFVESEHGDGDAFDGQGGTLAHAFFPVFGGDAHFDDAELWTIGSPRGTNLFQVAAHEFGHSLGLSHSDQSSALMAPFYRGFEPVFKLDEDDKAAIQSLYGK",
    "response#2": "GTPIVTVKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNSVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYR",
    "response#3": "ISQEMDNLVAEHPGLVSKVNIGSSFENRPMNVLKFSTGGDKPAIWLDAGIHAREWVTQATALWTANKIVSDYGKDPSITSILDALDIFLLPVTNPDGYVFSQTKNRMWRKTRSKVSGSLCVGVDPNRNWDAGFGGPGASSNPCSDSYHGPSANSEVEVKSIVDFIKSHGKVKAFITLHSYSQLLMFPYGYKCTKLDDFDELSEVAQKAAQSLRSLHGTKYKVGPICSVIYQASGGSIDWSYDYGIKYSFAFELRDTGRYGFLLPARQILPTAEETWRSIIARHR"
  },
  {
    "reference": "MDVFRALGNESLLSNSSGPARWDPFHHPLDSIQPWHFRLVAAVMFVVTSLSLAENLAVILVTFKFKQLRQPVNYVIVNLSVADFLVSLTGGTISFLANLKGYFYMGHWACVLEGFAVTFFGIVALWSLALLAFERYIVICRPVGNMRLRGKHAAQGIAFVWTFSFIWTIPPTMGWSSYTTSKIGTTCEPNWYSGAYNDRSYIIAFFTTCFIVPLLVILVSYGKLLQKLRKVSNTQGRLRTARKPERQVTRMVVVMIIAFLICWMPYAVFSILATAYPSIELDPHLAAIPAFFSKTATVYNPIIYVFMNKQFRMCLIQMFKCSAIETAESNMNPTSERATLTQDKRDSQLSVMAVRSTIS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should contain one or more G-protein coupled receptors family 1 profile domains that are essential for its function.2. The protein should be optimized for plasma membrane-specific folding and stability, while maintaining G protein-coupled photoreceptor activity. The designed protein sequence is ",
    "response#1": "ALEAMLYTLDQVNKQQLLPNVTLGAHLLDDCDKDTYGLEMAVDFIKGSISNIDDAEYHCNKTQVRKVISGVVGAASSVTSIQVANLLRLFRIPQVSYFSTSPELSNKQRFEYFSRTIPSDHYQVKAMVEIVKRMGWSYVSIIYEESNYGIKAFEELEELLARHNICIAIKEKLVKDSGVAEDIAYDNIVQKLLTKPRARGAIIFGSDQEVRQVMRAVRRANATGSFSWIGSDGWSARNLVSDDYEPEVEGTLSVQPQANPVRGFEEYFLSLTVENNQRNPWFVEFWEDHFQCRYPGSTSTPYNNYTKQCTTKERLSRQNTDFEDQLQFVSDAVMAFAYALRDMHRDLCGGGPSLCEAMKPTKGADLLKYLRKVEFEGLSGDEFRFDGNGDGPARYNIIHFY",
    "response#2": "GNGMVIFIFSTTKSLRTSSNFLVLNLAILDFIMMAKAPIFIYNSAMRGFAVGTVGCQIFALMGAYSGIGAGMTNACIAYDRHSTITRPLDGRLSEGKVLLMVAFVWIYSTPWALLPLLKIWGRYVPEGYLTSCSFDYLTNTFDTKLFVACIFTCSYVFPMSLIIYFYSGIVKQVFAHEAALREQAKKMNVESLRANQGGSSESAEIRIAKAALTVCFLFVASWTPYGVMALIGAFGNQQLLTPGVTMIPAVACKAVACISPWVS",
    "response#3": "ALEAMLYTLDQVNKQQLLPNVTLGAHLLDDCDKDTYGLEMAVDFIKGSISNIDDAEYHCNKTQVRKVISGVVGAASSVTSIQVANLLRLFRIPQVSYFSTSPELSNKQRFEYFSRTIPSDHYQVKAMVEIVKRMGWSYVSIIYEESNYGIKAFEELEELLARHNICIAIKEKLVKDSGVAEDIAYDNIVQKLLTKPRARGAIIFGSDQEVRQVMRAVRRANATGSFSWIGSDGWSARNLVSDDYEPEVEGTLSVQPQANPVRGFEEYFLSLTVENNQRNPWFVEFWEDHFQCRYPGSTSTPYNNYTKQCTTKERLSRQNTDFEDQLQFVSDAVMAFAYALRDMHRDLCGGGPSLCEAMKPTKGADLLKYLRKVEFEGLSGDEFRFDGNGDGPARYNIIHFHQ"
  },
  {
    "reference": "MKSCEHELLKTRRGKSREVSSRFLSSPSASSSPNRRNSTSNSSRDDQNNNGVKGHLGLKKHDRMSDGTRVCFGLPNQSSIEVDTKENRMPSPWINDEDNVILPGRFSVDECALYRASSRRNSCSLLYESFNDETDSELSDVSCASSLSTNRSSWNHKPGIKVSSKYLHDLTAKPSKGNNKTKLRSQDDSQRTNSSKGIENRLQRNNSVSRYGSSMSQWALSPGRSLDTQAVTVPSSKLKPPRGKGVGKLINLGFDFFRSKNKSSPFTSPLKPKTCDTESAHQLKLMNNRLLQWRFVNARACDVNKNVASQEKNQLLCAWDTLIKLNNLVLQERIKLQKKNLEMKLNYVFLSQVKHLEAWEDMEIQHLSSLSIIRDSLHSVLSRLPLKEGAKVNLESAVSIIKNAEAVTDAIISTVDDYAPTMEGIVPLASQLAEVVVQEKLMLEKCHDLLRMISELEMQERSLKCCFLIQHKQTFDTNLLKH",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm that is critical for spindle assembly.2. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "PGLQRSKSDLSERFSRAAADLERFFNFCGLDPEEARGLGVAHLARASSDIVSLAGPSAGPCSSEGGCSRRSSATVEERSLDRVPYGVSVIERNARVIKWLYGLRQR",
    "response#2": "LDRLQATMAPWFEGRETEMNFQRREEYTILIRRITHGNAPQRWPTAYLGFVKMTLPFVFITANSVRTSLQTAGLRAVQSLARVNKERLDSSIHIVLQNVLKLCANAKGITSQNSNLTIVAILENVTCNQRVLNQITGAAKDKNQNMRIFSAGWLEIIIDGQNRHKTHAEGVNSIAACIQEGVEDAKEDIRRAYRHTFFRFSSVWPARAKKILDAVSPKTQKLIS",
    "response#3": "LDRLQATMAPWFEGRETEMNFQRREEYTILIRRITHGNAPQRWPTAYLGFVKMTLPFVFITANSVRTSLQTAGLRAVQSLARVNKERLDSSIHIVLQNVLKLCANAKGITSQNSNLTIVAILENVTCNQRVLNQITGAAKDKNQNMRIFSAGWLEIIIDGQNRHKTHAEGVNSIAACIQEGVEDAKEDIRRAYRHTFFRFSSVWPARAKKILDAVSPKTQKLIA"
  },
  {
    "reference": "MTNPKNVVPNSGTSSRHASIVEMLSSPPLLPHQHAQHAHVHRSQGRSESIESIPLQRSASGTSSITSMGSNNDSSSANAQSANPSAGSATNVGASGTPHSGSGLEAGNTDLSAVPMLQTPSQCLNSAHIHTWKHIQLSQLIEQNKLIFVPGSLSVEETFNTLIKYHLTSIPVEAVPGDMNCLTFDYNDLNSYLLLVLNKITINNKQVTQDCQNGKPVAVGDIVKLTPKNPFYKLPETENLSTVMGILGSGVHRVAITNPEMTQIRGILSQRRLIKYIWDNARSFGTLEPLLNSSLQDLKIGVLNTNSKPTSRQSRVISIQGEEPLIMALYKMHKERISSIAVIDPQGNLIGNISVTDVKHVTRTSQYPLLHKTCRHFISVILNSRGLETGKDSFPIFHVYPTSSLARTLAKLVATKSHRLWIVQPQEAPTATSSSSTSSSVNTGTISPNPTPTTTSASPAPGATPNALNNHSPLLSTVDDGHTVINPATQATNTTVIQPNLFEKEYRTGKLIGVVSLTDIINLLARKQTANTEVDPQTARRQRGPATQ",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must be able to regulate endocytosis, septum digestion after cytokinesis, regulation of mitotic metaphase/anaphase transition.2. For general function, the protein need meet that Involved in DNA replication and cell separation. The designed protein sequence is ",
    "response#1": "TLRAMETNPAERPSPYFLETTQGGRMTALVRASMIAFMDEFSRFYELADGTLQRAAYFLDRYLSVTPESDDVLQLRLVGATAVFLAAKYEDQYTLRKIDASMVAARCGYTSETRHKMVSCMETEILAALDYNLCV",
    "response#2": "TLRAMETNPAERPSPYFLETTQGGRMTALVRASMIAFMDEFSRFYELADGTLQRAAYFLDRYLSVTPESDDVLQLRLVGATAVFLAAKYEDQYTLRKIDASMVAARCGYTSETRHKMVSCMETEILAALDYNLL",
    "response#3": "TLRAMETNPAERPSPYFLETTQGGRMTALVRASMIAFMDEFSRFYELADGTLQRAAYFLDRYLSVTPESDDVLQLRLVGATAVFLAAKYEDQYTLRKIDASMVAARCGYTSETRHKMVSCMETEILAALDYNLC"
  },
  {
    "reference": "METPQNNFFTFDSIDAALADIKAGRAIVVVDDENRENEGDVICAAQFATPDMINFMAVEARGLICLAMTGQRLDELDLPLMVTKNTDSNQTAFTVSIDAAKHLGGSTGISAEDRARTIQIAINPASKPEDLARPGHIFPLRAKTGGVLKRAGHTEAAVDLSRLAGLYPAGVICEIQNADGSMARLPQLVEYARKHNLKLISIADLISYRLKHDRFVYRETVCKFPSQFGDFQIYAYRNVLDETEHLAIVKGNPAQFDRQPVMVRVHSECLTGDALGSLRCDCRMQLQAALKMIEAAELGVVIYLRQEGRGIGLVNKLKAYTLQDLGLDTVEANERLGFPADLRDYGMGAQMLNDLGVKKIRLITNNPRKIAGLKGYGLEIVDRVPLLIEANDYNSEYLATKAKKLGHLLLQTYLITVAITWKQEIASATARYEKLDKIRHLAQSFDLLLQEEARPVAIALFSNPSLIFHLGFDQSNLAKSDWYRDCNHPYLEGIGKILDYLASWQEVQSLEFLIASGEDPMMGLQVKLDRQNFPLTQPPSQLCGKWESQIIYSFS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The Proton acceptor; for GTP cyclohydrolase activity active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The Mg(2+), GTP, D-ribulose 5-phosphate, Zn(2+) binding site should be stable and able to withstand changes in environmental conditions.3. A protein with GTP binding, magnesium ion binding, GTP cyclohydrolase II activity, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity functions is required to enable riboflavin biosynthetic process.4. The protein should be optimized for activity in riboflavin biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4, Cofactor biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "EKVAEAKLPTKWGNFKLVGFLDKITGAEHAALIMGNVNGEDSILCRVHSECLTGDTFGSLKCDCGDQFSAAMTKIAEEGRGVLLYLRQEGRGIGLLNKIKAYALQDRGMDTVDANLALGLPADARDYNCGIQMLKELGIKKIRLLTNNPDKINQINGADTGIEITERIPFADYYV",
    "response#2": "RVDSAKLPTKYGEFTAVAYESLLDNKGHIALVRGDLKSVDAPLVRVHSECLTGDVFGSQRCDCGDQLAAAMRMIDREGVGVLLYMRQEGRGIGLLNKIRAYKLQDMGKDTVEANEELGFPADLRDYGLGAQILADLNLTRIRLITNNPRKIAGLEGHGLVVAGRVASPEDIIERVAEARIPLSAGTFRAVGYDSLLDGIEHIAFVYGEIGDGEDILVRVHSECLTGDVFGSLRCDCGPQLEAALEAVAKEGRGVVLYIRGHEGRGIGLLHKLQAYQLQDAGADTVDANLQLGVPADARDYGTGAQILCDLGVRSMRLLTNNPAKRVGLEGYGLRVTGRSTRIPLQNKGDFNMTVFANELDCAEHFALVKPPAFANRVPLVRIHSECITGDVFGSCKCDCGKQLEQSLSLIAAEGGILIYLRQEGRGIGLANKLKAYALQEQGLDTVDANHQLGLPVDGRDYAIAYQILKYFGIDAIRLLTNNPSKIAAVDRYGIKVMERVEAEAKLPTKYGCFKVVGYRNKINGKENLAIVKGNIQNEKEVLVRVHSKCLTGDVFGSLRCDCGNQLSAALKAIEKHGSGVLLYMDQEGRDIGLLNKLKAYELQEKGMDTVEANLELGFPPDLRDYWGAAEILKDLGVKRIKLMTNNPDKIEKLQKYGIDVVKRVAEEYL",
    "response#3": "RVADARIPTEHGTFRAVGYQSSFDEAEHVALVMGDIAADGGEDVLVRVHSECLTGDVFGSLRCDCGPQLDAAMEMVGREGRGVILYMRGHEGRGIGLMHKLQAYQLQDNGADTVDANLELGLPADARDYGLGAQILVDLGVRSMRLLTNNPAKRVGLDGYGLEIVERSTRIPLQNKGDFNMTVFANELDCAEHFALVKPPAFANRVPLVRIHSECITGDVFGSCKCDCGKQLEQSLSLIAAEGGILIYLRQEGRGIGLANKLKAYALQEQGLDTVDANHQLGLPVDGRDYAIAYQILKYFGIDAIRLLTNNPSKIAAVDRYGIKVMERVSTRIPLQNKGDFNMTVFANELDCAEHFALVKPPAFANRVPLVRIHSECITGDVFGSCKCDCGKQLEQSLSLIAAEGGILIYLRQEGRGIGLANKLKAYALQEQGLDTVDANHQLGLPVDGRDYAIAYQILKYFGIDAIRLLTNNPSKIAAVDRYGIKVMERVR"
  },
  {
    "reference": "MTTPIGAWSEVGKLREVMVCEPRLAHRRLTPSNCRELLFDDVLWVEKAQEDHRDFVAKMRERGIVVHEMHQLLSEVLDISEGRSWVLDRKLSPNAVGLGVDEDVRDWLNDMPSDTLAEYLIGGVAYGEVPADRRKHVLGALIKAHSENEFLIPPLPNTQFTRDTTAWIFGGVTFNPMRWPARQQETVLASAIYTHHPIFAERDFKIWYGCAEADHGMSTLEGGDIMPVGNGTVLIGMGERSSWQAITQVARSLFAQNAARRMVVAAMAPDRASMHLDTVFSFCDVDLVTLYKPVVDTITPLVLEPSSEAAGFSVRVDDRHFTDVVASSLGLDGLRTVETGGDCWEAQREQWDDGNNVVALEPGVVVGYDRNTATNALLAKAGVEVVEIKAAELGRGRGGGHCMTCPITRDPVDY",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must have a Amidino-cysteine intermediate active site that is highly conserved among related enzymes.2. The designed protein should interact with enzymes and substrates within the carbamoyl phosphate from L-arginine: step 1/2 pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "LIDCGVKENILRSLVSRGASVTCFPFDYPIHKVAHHFDGVFISNGPGDPTHCTSTVYNLRKLFETSQLPVMGICMGHQLIALAAGAKTIKLKYGNRAHNIPALDLTTGKCHITSQNHGYAVDPTTLTSEWKEYFTNLNDQSNEGLIHASRPIFSAQFHPEAKGGPMDSSYLFDKYIS",
    "response#2": "LIDCGVKENILRSLVSRGASVTCFPFDYPIHKVAHHFDGVFISNGPGDPTHCTSTVYNLRKLFETSQLPVMGICMGHQLIALAAGAKTIKLKYGNRAHNIPALDLTTGKCHITSQNHGYAVDPTTLTSEWKEYFTNLNDQSNEGLIHASRPIFSAQFHPEAKGGPMDSSYLFDKYIEG",
    "response#3": "LIDCGVKENILRSLVSRGASVTCFPFDYPIHKVAHHFDGVFISNGPGDPTHCTSTVYNLRKLFETSQLPVMGICMGHQLIALAAGAKTIKLKYGNRAHNIPALDLTTGKCHITSQNHGYAVDPTTLTSEWKEYFTNLNDQSNEGLIHASRPIFSAQFHPEAKGGPMDSSYLFDKYITQ"
  },
  {
    "reference": "MGKREIHFTPVGRQVQRVLEYPLRLENRSPMSYSRRSRYSPSLSPYDKRRGRSVSRSLSRSPTRSVSSDAENPGNSLYVTGLSHRVTERDLEDHFAKEGKVTDVHLVLDPWTRESRGFGFISMKSVGDANRCIRSLDHSVLQGRVITVEKARRRRGRTPTPGKYLGLRTARGRHKSPSYSPRRSVSCSRSRSRSYSSDRGRSYSPSYGRRGRSSSYSPFYRRRRFYSPSRSPSPDDRYNRRRDRSYSPYYRRRDRSRSYSRNCRARDRSPYYMRRYRSRSRSYSPRYRARDRSCSPYYRGRDRSYSPHYQGRDRSYSPESRYYRRHRSVSGSVSPGGRSMSRSISPRKGRKESRSKSRRHDRQSSMCHSRSARSSTSRSVSP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Target a Polar residues compositional bias in the protein's composition for improved properties.2. For general function, the protein need meet that Probable splicing factor involved in constitutive and/or alternative splicing events3. The protein should be optimized for stability and expression in spliceosomal complex where it will participate in regulation of RNA splicing, mRNA processing, positive regulation of mRNA splicing, via spliceosome, response to high light intensity. The designed protein sequence is ",
    "response#1": "VKSGTQRSVKVIEHKKDPLQPVTHRKRKVVVPSEEIQAPILHKSDDPSIKPTKEELDKWRIPSAISNWKNPNGFAISLDNRVAIESIKTDCPDNDKKDNFLLLSEALDEAEREARQRINIKQEAYKELEKEETLKKEQRLRHLAERARQDR",
    "response#2": "VKSGTQRSVKVIEHKKDPLQPVTHRKRKVVVPSEEIQAPILHKSDDPSIKPTKEELDKWRIPSAISNWKNPNGFAISLDNRVAIESIKTDCPDNDKKDNFLLLSEALDEAEREARQRINIKQEAYKELEKEETLKKEQRLRHLAERARQDS",
    "response#3": "VKSGTQRSVKVIEHKKDPLQPVTHRKRKVVVPSEEIQAPILHKSDDPSIKPTKEELDKWRIPSAISNWKNPNGFAISLDNRVAIESIKTDCPDNDKKDNFLLLSEALDEAEREARQRINIKQEAYKELEKEETLKKEQRLRHLAERARQDL"
  },
  {
    "reference": "MATQLIDLAQQTRAAAQKLGTLSLAQRNDALAKVAQALAANQAKIVAANQADCEAAQRDGIAPALYARLKLGESKLQGAIAGIHDVINLPDPVGHLQLHRELDQDLVLKRVTCPLGVLGIIFEARPEALIQITSLAIKSGNGVILKGGKEAIQSCTVLTEIIQTALQDTAVSPQAVTLLTTREEIKTLLSLDQYVDLIIPRGSNAFVQYVQQNTTIPVLGHADGVCHLYVDVAADLSKTIPIVVDAKTQYPAACNAVETLLIHEKIAPEFLPQIAAALTAKQVTLRGDAATQKIMPVQPATAEDWRTEYSDLVLAIKIVPDVEAAIAHINTYGSKHTDGIITEDAATAQIFLNEVKAAGVYHNCSTRFADGFRYGFGAEVGISTQTLPPRGPVGLEGLVTYKYHLVGNGQIAATYSGPDAKPFTHRDL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that can modulate the overall flux of metabolites through the L-proline biosynthesis pathway, enhancing or suppressing its activity as needed.2. The protein must exhibit the following characteristics: The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.; Catalyzes the NADPH-dependent reduction of L-glutamate 5- phosphate into L-glutamate 5-semialdehyde and phosphate.3. The protein must be able to glutamate-5-semialdehyde dehydrogenase activity.4. The desired protein should be able to perform L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + L-   glutamyl 5-phosphate + NADPH The designed protein sequence is ",
    "response#1": "MLEQMGKQAKDAAFILAQLTTAEKNCALSIIAEQLQQQAPLILAENAKDIELAKQNGLSDALIDRLLLTQERLQSIANDVRHVISLADPVGKIIDGGTLDSGLKIERVRTPLGVIGTIYEARPNVTIDVASLCLKTGNAVILRGGKETQFSNKILIEVVQNALEQAGLPKFAVQAITDPNRELVMQLLKLDRYVDMIIPRGGSGLHELCKQHSTIPVIVGGVGVCHTFVEKSADQNKAIFVIDNAKTQRPSTCNTLETLLVQHSIAEEFLPKLVSHLATKNVKYHAKSTALNILKQAGANVCEVTEKELRKEWGSLDLNVVVVEDIHAAIEHIRQYGTQHSESILTSSQNLARQFINQVDAAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTSYKWVCEGEYTVPF",
    "response#2": "VQAQATRAAARQLAQWSGADRQRLLSAIATTLEQEAPRILAANQADCEAATTEGIAPALYARLKLDADKLAAAIAGVRDLAQLPDPLGQIQIDRELDEGLILQRLTCPVGVLGVIFEARPDAVIQIASLAIKSGNGAILKGGREAICSCQAIVAAIAQALAEQQAPVEAIRLLTSREETLALLKLDRYVDLIIPRGSNSFVRFVQDNTHIPVLGHADGICHLYVDQAAAIEKTVTITVDAKTQYPAACNAIETLLIHEAIAPQFLPVVAAALHRLAAEVRQAAMALGQSDDNQRRKALMAMANALLSSSEQIVRANQLDLEKARTEGLASALMARLRLDESKLNSAIEGLRQLAQLTDPLGLRQLHRELDQDLVLERITVPLGVLGVIFEARPDAVIQITSLAIRSGNGALLKGGSEASHTNQAIIEALKNGLAETEIDPEAIALLKTRQESLALLRLDGLVDLIIPRGSNELVRFIQDNTRIPVLGHADGICHLYLDAAADLKQALQIAIDSKTQYPAACNSIETLLIHQSIAPSFLELAIPAFLQAGVRLLGDSASRALGVEESASEEDWATEYLDLILSVKVVPDLEGALDHIRRYSSRHTEAIVSNDQETAERFLQAVDSAGVFHNCSTRFADGFRYGFGAEVGISTQTLPPRGPVGLEGLVTYRYRLRGDASVVADP",
    "response#3": "QEMGKQAKQAAFVLAQLSQQQKNQALEIIAQQLEAQSDKILAENQKDIALAKQNGLSDAIIDRLLLTPSRLNDIANDVHHVISLADPVGQLIDGGILDSGLKIERVRVPLGVIGTIYEARPNVTIDVASLCLKTGNAVILRGGKETQYSNKILVDVVQHALVQAGLPKYAIQAITDPDRHLVMELLKLDRYVDMIIPRGGASLHELCKQHSTIPVIVGGIGVCHLFIEQSADLDKALPLIENAKTQRPSTCNTLETLLVQRAIATQFLPKLAQHLSAKHVKFIS"
  },
  {
    "reference": "MQLVFATNNLNKLKEVQSLIPSSIKLLSLKDIGCFEDVPETQNTIKGNAVQKAEYIKEHYGYNCFADDTGLEVEALNNEPGVFSARYAGPKRDANDNMDLLLKNLKEKPNRKAQFKTVVSLNLNGELKTFTGICKGEITLDKKGDQGFGYDPIFMPNGYTETFAEIDLKLKNKIGHRGKAVTKLVQFLSTL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein with XTP diphosphatase activity, ITP diphosphatase activity, nucleotide binding, metal ion binding, ribonucleoside triphosphate phosphatase activity functions is required to enable purine nucleoside triphosphate catabolic process, nucleotide metabolic process.2. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.3. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.4. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+), substrate binding.5. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MKLVFATNNKNKLREVQAALPSDIEILTLDDIGCTEEIPETADTIEGNALLKAKYVTEHYGYDCFADDTGLEVEALNGEPGVYSARYAGPERDARDNMDKLLTNMQGKTDRSAQFKTVISLNLEGKQHLFEGIVNGTILTEKRGDKGFGYDPVFMPDGMEQTFAEMPLAEKNKISHRGKAVQKLVAYLKTM",
    "response#2": "MSILSGKXVAVTGAASGIGRAVVEALLDAGAE",
    "response#3": "MKILVVTGNKNKLREVRDILSDVDIRVISLDEIGCEEEIEETGLTFEENAKIKALFVKEYYEVPVISDDSGLMVDALNGMPGIYSARYAGEGASYSDNIRKLLEAMEGLEDRKAYFVCCIAIVFPDGKVLTFRGEVKGYIASEMRGNNGFGYDPIFIPEGYEKTFAELGEEIKNLISHRAKAFKAAKEAILKIL"
  },
  {
    "reference": "MKVLINKNELNKILKKLNNVIVSNNKMKPYHSYLLIEATEKEINFYANNEYFSAKCTLAENIDVLEEGEVIVKGKIFSELINGIKEDIITIQEKDQTLLVKTKKTNINLNTIDKKEFPRIRFNQNVDLKEFDELKIQHSLLTKGLKKIAHAVSTFRESTRKFNGVNFNGSNGKQIFLEASDSYKLSVYEIKQKTDPFNFIVETNLLSFINSFNPEGGDLISIFFRKEHKDDLSTELLIKLDNFLINYTSINESFPRVMQLFDFEPETKVTIQKNELKDALQRILTLAQNERFFLCDMQVTNSHLKINSNVQNIGASLEEVTCLKFEGHKLNIAVNALSLLEHIDSFDTDEIELYFQGSNKYFLISSNNEPELKEILVPSK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein can be expressed in cytoplasm, DNA polymerase III complex and perform DNA binding without triggering undesirable cellular responses.2. The protein must exhibit the following characteristics: Confers DNA tethering and processivity to DNA polymerases and other proteins; The beta chain is required for initiation of replication as well as for processivity of DNA replication.. The designed protein sequence is ",
    "response#1": "DFPHLLVYGPSGAGKKTRILAVLQEIYGPNALKLKIDHRTFKHPTSSKNIQITTISSHYHIEINPGEAGSYDRVVIQTIIKEIAQSPPIDSGSLGAFKIVILNEVDKLSKDAQHALRRTMEKYATFCRLILCCDSTAKVIDPIKSRCLGIRVPAPSQEEIEKVI",
    "response#2": "IWFACGPYTATDNCGYEHLCELLDKVVAEKPDILMLAGPFVDKKNTFLNKPTFNITYDNLLEDLLLKVKETLVGTKTQVIIQPNASRDLCVPPVFPSAPFQQNRKLDKIKKELIFVADPCIFRISGVEVAMTSSEPIQALSNTEFHRSANQENIDRVARLSSHLLTQQCMYPLEPTEVPASMGDLLDVCCIGSTPHIVFAPTKLAPSFVCISEVHLDNHQTFYALEKIFQKYESSEAVPFCIILCGSFMSSAFHNSGSSIQYKEGFNKLAASLEKFPKICEKTKFIFVPGPNDPWTTNGISLMPKHSIPLHFVNRIQRVCKHTIFASNPCRIIFFSQEVLIYRDDISGRFQRNSLKFGKTPQGTSNINSIPLAEQQVHQQRKLVKTVLDQSHLSPFPSRTRPILWDFDYALSVFPLPSCMGLIDSESSAHVFGTNTSSLELFLLSRKIKGPSWLEIKSPQLSSQPMSWCKVEAVVTRPDQVSVVKDLAPPPVVVLSLSMKTVQNAKTHQNEIVAIAALVHHTFPLDKAPPQPPFQTHFCVLSKLNDCIFPYDYNEAVKQKNANIEIALTERTLLGFFLAKIHKIDPDVIVGHDIYGFDLEVLLQRINSCKVPFWSKIGRLRRSVMPKLGGRSGFAERNAACGRIICDIEISAKELIRCKSYHLSIWFACGPYTATDNCGYEHLCELLDKVVAEKPDILMLAGPFVDKKNTFLNKPTFNITYDNLLEDLLLKVKETLVGTKTQVIIQPNASRDLCVPPVFPSAPFQQNRKLDKIKKELIFVADPCIFRISGVEVAMTSSEPIQALSNTEFHRSANQENIDRVARLSSHLLTQQCMYPLEPTEVPASMGDLLDVCCIGSTPHIVFAPTKLAPS",
    "response#3": "IWFACGPYTATDNCGYEHLCELLDKVVAEKPDILMLAGPFVDKKNTFLNKPTFNITYDNLLEDLLLKVKETLVGTKTQVIIQPNASRDLCVPPVFPSAPFQQNRKLDKIKKELIFVADPCIFRISGVEVAMTSSEPIQALSNTEFHRSANQENIDRVARLSSHLLTQQCMYPLEPTEVPASMGDLLDVCCIGSTPHIVFAPTKLAPSSEYYAVLKKSDFERGLRAAEDISDSIRFTLSQDGFKAYSHSESEESEMILPKDLITDMSCNTTIKSSYPLEYLLKFIKAISSTDSLKLSFRDDYPLSVEFYLDN"
  },
  {
    "reference": "MPLMGRKIKVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNGLDALRVIMKRYPVPVIMISALTQEGADATIKALEYGAIDFIPKPSSSISINMKELKDEIIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSLLKIFPKFPEDLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPGDYHMEVTLRAGKPVITLNKKPKIHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIVEIKKKGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVKAVEIIRGGGNLGRHVTISR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The presence of CheB-type methylesterase, Response regulatory domains is critical for the interaction of the designed protein with its intended targets.2. For general function, the protein need meet that Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR; Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid.; Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli; Involved in chemotaxis3. The designed protein should be involved in chemotaxis, protein demethylation, protein deamination by localizing to cytoplasm and exhibiting protein-glutamine glutaminase activity, protein-glutamate methylesterase activity, phosphorelay response regulator activity.4. The protein with the reaction H2O + L-glutaminyl-[protein] = L-glutamyl-[protein] + NH4(+), the active site must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MNIGIVNDLPLAVEALRRVIALRADHRVLWVATDGDEAVDFCVAHPPDVVLMDLVMPKVDGVAATRRIMARAPCAILIVTASVSANTSSVYEAMGAGALDAVDTPTLALGLSTDASPQALLAKVCIGVSTGGTEALRDVLECLPAKAPAILIVQHMPQGFTAAFAKRLDSLCEIEVREAQDGDVVVQGCAYIAPGGRHMLLQRTGQRYHIAIKDGPPVSRHRPSADVLFRSAAQYAGPNALGIIMTGMGDDGARGLLEMHKLGATTRAQDEESCVVFGMPKEAIALGGVDKVVPLAQIPREGVTAPRVLLIGSSTGGPQALMSLVSEIGSVIDHYPVLITQHMPPTFTTILAEHLMRSARRPAHEGVDGEVVKSGQIYLAPGGRHMRVVRQGGQAVIALDDGPPVNFCKPAVDPLFNSAIDVWQSGILAVVLTGMGSDGMRGGKDIVAAGGSVIAQDEASSVVWGMPGAAANAGICAAVLPLNQIGPKLVRLFSGG",
    "response#2": "LLLIGTSTGGPVALQKVLTAFPANYPHPIVLIQHMPAAFTPAFAARLNTLCKIEVKEAENGDVMRPGCAYLAPGGMQLMIERSGISGRLKVISGTQEMNYKPCVDITFASASKAFGGDVLAVVLTGMGADGREGARMLKSVGATIWAQDEASCVVYGMPQAVASTGISTHSISLDNMAEAILSRA",
    "response#3": "LVIVGASTGGTEAIREVLQPLPADSPAILITQHMPAGFTRSFAQRLDALCAVTVREASDGERVLPGHVYLAPGGETHMRLGRSGANYVIGLQASEPVNRHRPSVDVLFHSAAEAAGGNAIGVILTGMGKDGAAGLLAMKRAGARTMAQDEASCVVFGMPREAIALGAADEVVPLADISERILALPDEYPLVVIGASAGGPPSLAAVLRALPASFPAAIVLVQHVDAAFTSGMAAWLNEQCVLPVRLASDGLRPEAGVVLLAGAGEHLVLGAERKLHYTTEPRESVYSPSIDVFFHSVAQHWRGRAAGVLLTGMGQDGALGLKAMRERGFFTIAQDRATSAVYGMPKAAAALDAACEILPLGDIAPRPVSPTRPGVVALAASTGGPAALFRLLSELPASFPVPLLVVQHIAIGFSEGLAQWLRTAGPLPVKVAEDGEPLLPGHVYLAPDDRHLGVRGEGRAEVSRAAPVNGFRPSATWMFRSVARAYGPASLAVILTGMGQDGLEGVRELHGAGGRILAQDEQSSVVYGMPGVVVGANLAHEVVALPDLASRLTSAFRGG"
  },
  {
    "reference": "MTEPAQKKQKQTVQERKAFISRITNETKIQIAISLNGGYIQIKDSILPAKKDDDVASQATQSQVIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAFKEAMGAVRGVKRFGTGFAPLDEALSRAVVDLSNRPFAVIDLGLKREMIGDLSTEMIPHFLESFAEAARITLHVDCLRGFNDHHRSESAFKALAVAIREAISSNGTNDVPSTKGVLM",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must be able to regulate histidine biosynthetic process.2. A protein should be able to manipulate the activity of enzymes in the Amino-acid biosynthesis, L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9 pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "ASEIAGEIGQGAVDLGITGEDLVRENLADWEARAEIAARLGFGHADVVVAVPEIWLDVDTMADLDDVAADFRQRHGRRLRIATKYWRLTQQFFSQKHGIQVYRIVESLGATEGAPAAGLADVIVDITTTGSTLRANHLKVLGDGVVLKSQACLVASKKARTAGDEATLRDIVTKI",
    "response#2": "LVYSSKESVAESLKTGTGVYQSRKRGLWYKGASSGAVQHLIHIDVDCDEDCLRFVVYQTGKGFCHLDTLHCH",
    "response#3": "LVYSSKESVAESLKTGTGVYQSRKRGLWYKGASSGAVQHLIHIDVDCDEDCLRFVVYQTGKGFCHLDTLHCR"
  },
  {
    "reference": "MYPGKVYRGVKEIRGSLLIVDGIEEAAYDEVVKIYGKDSRERFGRVLETSIGQAVVQVLGDREGLETDTLLKFTGSTFKIRVSEDVIGRVFNGRFEPIDGLPPILSGELREITGEPINPISREYPHDFIQTGVSAIDGLFSMVRGQKLPIFSVSGLPHNLLAAQVARQATVRGEGEQFAVVFAGIGLRKTEAEFFLEQFRETGAIERLVAVLNMADDPAVERLMTPRIALTVAEYLAFDLDMHVLVIMSDMTNYCEALREVSSARGEIPGRLGYPGYMYSDLATIYERAGVIKGKKGSITLFPILTMPGGDLRHPIPDLTGYITEGQIFLSQEMYAQGIYPPINILPSLSRLMKSGIGPGKTREDHRYLADQLYDAYSRGVKARDLARIIGEIGLSERNRRFLKFAEEFENKFVNQGFYENRSIEETLDLGWQVLSILPEEELVRIPQKIIEKYHPKYRS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein with ATP binding is needed to enhance proton motive force-driven plasma membrane ATP synthesis in a specific context.2. For general function, the protein need meet that Produces ATP from ADP in the presence of a proton gradient across the membrane The designed protein sequence is ",
    "response#1": "GISSIDHLNTLVRGQKLPVFSGSGLPHNELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLANDPAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYPGYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHELYNQGYRPPINVLPSLT",
    "response#2": "GISSIDHLNTLVRGQKLPVFSGSGLPHNELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLANDPAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYPGYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHELYNQGYRPPINVLPSLT",
    "response#3": "GISSIDHLNTLVRGQKLPVFSGSGLPHNELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLANDPAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYPGYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHELYNQGYRPPINVLPSLT"
  },
  {
    "reference": "MIIFMKDFEEQVMNLVNLLGISKDLFFILWTVLSILTLLLGVTMGVLVIVWLERKISAGMQQRMGPEYTGPLGILQTLMDGIKLLLKEDIVPAKGNTWLFNLGPAIVVIPVFLSYLVIPFGQKMILSDLGIGVFFWIAVSSIVPLGLLMTGYGSNNKYSFLGGLRAAAQSISYEIPLALCVLSISLLSNSLSTVDIVEAQSKYGILGWNLWRQPVGFIVFIVSSLAECERLPFDLPEAEEELVAGYQTEYSSIKFGFFFIGSYLNLLVSSLFVTVLYLGGWDLSLPFLPGLNHITMTWYSSDGITEVPSIFLSFFITLIKAFLFLFVSIAARWTLPRVRMDQLLDLGWKFLLPIALGNLLLTASFQLLLLG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein can be expressed in chloroplast thylakoid membrane and perform oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "RMYLLTFEGHLHIHFQNYNSIKNGSLYSISIWGKEGPKPVKINFVLSTMNNNEKVSFFSKKTYQIDGNVRNLMRSFSIHFYNKETSVYPQESDNTMLFPLLVLVLFTLFVGSIGISGVTDMDFDILSKWLTPSINLLHKNLSYSPDWYEFFINAIFSVSISYFGILIAFLLYGSVYSSFQNLDLINLFVKTGSKKILLDRIKNVIYNWSYNRGYIDVFYATALTRGIRGLAQLTHFFD",
    "response#2": "RMYLLTFEGHLHIHFQNYNSIKNGSLYSISIWGKEGPKPVKINFVLSTMNNNEKVSFFSKKTYQIDGNVRNLMRSFSIHFYNKETSVYPQESDNTMLFPLLVLVLFTLFVGSIGISGVTDMDFDILSKWLTPSINLLHKNLSYSPDWYEFFINAIFSVSISYFGILIAFLLYGSVYSSFQNLDLINLFVKTGSKKILLDRIKNVIYNWSYNRGYIDVFYATALTRGIRGLAQLTHFFN",
    "response#3": "RMYLLTFEGHLHIHFQNYNSIKNGSLYSISIWGKEGPKPVKINFVLSTMNNNEKVSFFSKKTYQIDGNVRNLMRSFSIHFYNKETSVYPQESDNTMLFPLLVLVLFTLFVGSIGISGVTDMDFDILSKWLTPSINLLHKNLSYSPDWYEFFINAIFSVSISYFGILIAFLLYGSVYSSFQNLDLINLFVKTGSKKILLDRIKNVIYNWSYNRGYIDVFYATALTRGIRGLAQLTHFFN"
  },
  {
    "reference": "MTNLQTGPTTRGLQRFAIPAVCGLIIFLGYYSQYLFNTSADLAPGPLTCRESLIFNILLVCLWLTYYQACTVDPGQYKFPPKEKEDGDNNNKRGGRGPQKAKWCKKCDAPKPPRAHHCRHCARCIPRMDHHCPWTGNCVSLQTFPHFLRFLVYTNAALVYFARLLWTRLYYGLWDQRHVPAYLGPSVGALLGCTMLSIAWFATQFALMVLLVTTVRSWMLGKTMIEEWEAERHETLLARSYDGDDYWGADGHGGFVPVKVEFPYDNGFWSNMAQAMGTNNFLRWFLPVGGGGPKISNDTPWKGTGWEYEENGFNDRVGMWPPPDPEKLRRERAGAGGKWPGARENLNTEKPEVDYYRSSEDMKTAFKRRQQEDLRRRQQRRQHSSEEDEIMAELEEDEGYEQRSRTRSPPQDGRAWMNSEGDTLWDYGVDVDEEENYGYPGQGVSESLPLVKTATGYNDGQGDEDEDVPLAELIRRRKVKSNGVHE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. We need a protein that can perform hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-   hexadecanoyl-L-cysteinyl-[protein] with high efficiency.2. The protein should exhibit Basic and acidic residues compositional bias.3. The protein should be optimized for stability and solubility when expressed in endoplasmic reticulum, Golgi apparatus, endoplasmic reticulum membrane where it will be involved in peptidyl-L-cysteine S-palmitoylation. The designed protein sequence is ",
    "response#1": "GMKCSTCQLIKPARSKHCRVCNRCVQRFDHHCVWVNNCIGAQNTRYFMLYLLSVCAMAGNIAVLTTDMLLQTVLRTGLLHAHYIDEQGIQQPAGPLFIIQHLFLTFPRIVFMLGFLVFVFFLLAGYCLFHFYLVLVNQTSNEWFKC",
    "response#2": "EEALFCTLCNAEVRKFSKHCRSCDKCVDCFDHHCRWLNNCVGRKNYMTFISLMAVSLLWLLIEAGVGIAVIVRVFVNKKDMETEIVNRLGNGFSRAPFATVVGLCTAVSMLALFPLGELFFFHMLLIKKGITTYEYC",
    "response#3": "KFCDTCQLYRPPRAFHCSICNNCVQRFDHHCPWVGQCIALRNYPFFVCFLSCSTLLCIYVFVFSWVSMLKVHGEFYVVLADDLILGVLGLYCFVSVWFVGGLTVFHFYLICTNQTTCENFR"
  },
  {
    "reference": "MEKLDKLSEAFKALLKQEKFGSQSEIVTALQELGFENINQSKVSRMLSKFGAVRTRNTKMEMVYQLPAELGVPTTSSPLKNLVVDIDHNDVLIVVKTSPGAAQLIARLLDSMGKSGGILGTIAGDDTIFITPTKVTPVEVLMQNVTELFESSF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be optimized for arginine binding, DNA-binding transcription factor activity activity in order to improve protein complex oligomerization.2. Ideal protein should have a binding affinity for key enzymes in the L-arginine biosynthesis [regulation] pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "GELLVSTDDSGNLAVLRTPPGAAHYLASAIDRAALPQVVGTIAGDDTILVVAREPTTGAQLAGMFS",
    "response#2": "DDLLVSVDHSGNIAVLRTPPAGAPFLASFIDRVGMDEVVGTIAGDDTVFVLARDPMTGKELGEFL",
    "response#3": "MNKGQRHIKIREIITNYEIETQDDLVDRLKSDGYNVTQATVSRDIKELHLVKVPLQDGRYKYSLPADQKL"
  },
  {
    "reference": "MKQFIFFALLCTSTYAAIHILTEKEDHATLHISFNDLIKIQLRTNPSTGYAWNIEYPTDTFSLSQDTIKAEPHPSGMVGFPSIREIQLKPLKVGTTTIKLGYSRPWEKGKEPLRSLTYSVVIR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design must feature the BC loop, FG loop, DE loop motif.2. A protein with cysteine-type endopeptidase inhibitor activity is needed to enhance negative regulation of peptidase activity in a specific context.3. For general function, the protein need meet that Cysteine protease inhibitor The designed protein sequence is ",
    "response#1": "FVDDSFPPGPESVGFPAGDSVQQRVRQWLRPQEINCSVFRDHRATWSVFHTLRPSDILQGLLGNCWFLSALAVLAERPDLVERVMVTRSLCAEGAYQVRLCKDGTWTTVLVDDMLPCDEAGCLLFSQAQRKQLWVALIEKALAKLHGSYFALQAGRAIEGLATLTGAPCESLALQLSSTNPREEPVDTDLIWAKMLSSKEAGFLMGASCGGGNMKVDDSAYESLGLRPRHAYSILDVRDVQGTRLLRLRNPWGRFSWNGSWSDEWPHR",
    "response#2": "FVDDSFPPGPESVGFPAGDSVQQRVRQWLRPQEINCSVFRDHRATWSVFHTLRPSDILQGLLGNCWFLSALAVLAERPDLVERVMVTRSLCAEGAYQVRLCKDGTWTTVLVDDMLPCDEAGCLLFSQAQRKQLWVALIEKALAKLHGSYFALQAGRAIEGLATLTGAPCESLALQLSSTNPREEPVDTDLIWAKMLSSKEAGFLMGASCGGGNMKVDDSAYESLGLRPRHAYSILDVRDVQGTRLLRLRNPWGRFSWNGSWSDEWPHV",
    "response#3": "GIVGGVSEAKPATPEIQEVADKVKRQLEEKTNEKYEKFKVVEYKSQVVAGQILFMKVDVGNGRFLHMKVLRGLSGDDDLKLLDYQTNKTKNDELTDC"
  },
  {
    "reference": "MKSLFYHFIGIGGIGMSALAHILLDRGYRVSGSDLLEGEIVLTLKNKGAKFFLGNREEHIPEGAVVVYGSGISQENPEFLSAKNRGHRLIHRAELLAELAKDQISIFVTGSHGKTTVSSLITAILQKLGTTPSFAIGGLNEDGINGSSGFEYFVAEADESDGSIQHYLPEFSVITNIDDEHLSNFGGDRGALLASLQDFAFRTRRSCWYNGDCSLLRSSLKGNTFGFSSSCDLHILSYRQEEWRVFFTAQYRGILYEDVEVQLAGEHNVMNAAAAMGIALSLGIDELTIRDALREFSGVQRRLQRKNVSETFLFLEDYAHHPSEISCTLQAVRAAVGQRRVLAICQPHRFSRLKECWGRFPSAFKYADEVFLTEVYGAGEREEAVPYQELAQSISRESLVKCTYVPFQELKRFLEQRIRVHDVCVALGAGNIVTLGESLQDFEPKKLSLGILCGGRSCEHDISILSAKNIARHLSSTFYEVQGFIITREGLWKKVSLLEGTEDSEKSIFDSEIAMQLERLDVIVPILHGPYGEDGAIQGFLETIGKPYTGPSVVFAAIGMNKVLTKRFMSDLGIPVVPYLPLTLTGWKQEPEKWLAQIMKAFSFPMFVKAAHLGSSIGVFEVHDALELREAIHEAFVQDNDVFIEESRLGCKEIEVSFLGDGAGVFFVAGMHERRGSGGFIDYCEKYGLSGKPSAQIVFDVDLSQEMQEQLLGAAEKIYRLLQGKGSCRIDFLVDDEGSFWLSEINPIPGTTESSPFLTAFVRKGWSC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should be able to enhance the efficiency of regulation of cell shape. The designed protein sequence is ",
    "response#1": "VTGTNGKTSVANFVANAANLLGXRCGVVGTLGAGXPDELDVXLTXGLTTPVAITLQSALASIVDQGCAMVXLEASSHGLSQRRLNGTSIDIAAFTNLSRDHLDYHDSMNAYLESKKQLLEWPHLKTAIFXADDPYCGAMCRXVSADRIFYGVTNKADVVASCIDISDQGVSFKLDSPWGASTVSSNLLGRFNISNLXASVCW",
    "response#2": "VTGTNGKTSVANFVANAANLLGXRCGVVGTLGAGXPDELDVXLTXGLTTPVAITLQSALASIVDQGCAMVXLEASSHGLSQRRLNGTSIDIAAFTNLSRDHLDYHDSMNAYLESKKQLLEWPHLKTAIFXADDPYCGAMCRXVSADRIFYGVTNKADVVASCIDISDQGVSFKLDSPWGASTVSSNLLGRFNISNLXASVCN",
    "response#3": "AAMASGVTMTDPDTVWLAADTRLAADVTIGPCVRFGPGVSVASNVEIKAFCDIEGARIGRGALIGPFARIRPGSEVAEDVHIGNFVELKATKMGKGAKANHLAYLGDSDIGARSNIGAGTIAVNYDGYGKYRTVVGAEAFVGSNSSLVAPVRIGKGANVTAGSVVTEDVPAGAVAFGRARG"
  },
  {
    "reference": "MDGGAEGSQQPHLILANKLFLLTHPDVPDIEKVQLKSEVLDFIRSHGMAPLYETLIASSVLDLDQSLLESMRAANEEELKKLDEKIADAEENLGESEVREAHLAKALYFIRISDKEKALEQLKLTEGKTVAVGQKMDVVFYTLQLAFFYMDFDLVSKSIDKAKKLFEEGGDWERKNRLKVYEGLYCMSTRNFKKAASLFLDSISTFTTYEIFPYETFIFYTVLTSIITLDRVSLKQKVVDAPEILTVLGKIPFLSEFLNSLYECQYKAFFSAFAGMAVQIKYDRYLYPHFRFYMREVRTVVYSQFLESYKSVTVEAMAKAFGVSVDFIDQELSRFIAAGKLHCKIDKVAGVLETNRPDAKNALYQATIKQGDFLLNRIQKLSRVIDL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.2. Design the protein with a signal peptide incorporated.3. The designed protein should contain one or more PCI domains that are essential for its function.4. The protein should be engineered to have a higher affinity for a specific molecule or receptor in proteasome complex that is critical for proteasome-mediated ubiquitin-dependent protein catabolic process, protein catabolic process. The designed protein sequence is ",
    "response#1": "ENKHLMTGLHLMFLLAANRLSDFHMLLEQIPQKEQTSNAYISTPVRIEQSLMEGAYNKVVLTEKNIPSPFYTIFIRIMLDTIRREIATSIEKSFKVLTAKDATVMLLFDNDEQMKKFGQERKWHLDGERYVFEIEVAQEKPR",
    "response#2": "KMPLLQSSVALLGQKIRLMALIELVFQLPPNQRTLTFDTIARATRIPSNEVELLIMRALSVGLITGVIDEVTQIVTIQ",
    "response#3": "ILMEDLYERKLWKQLTDALIVFFDTPETVPLRLPLYTNFVNSFRPNINQLKAVYMGLKAFESCSNDEALRNLNQIVNELDEEKYKDAYVYSIVAIARIKLISGKLDEARELLVKASKIIDHIDYVESLIHSSYYSVSADYYKAKADYAQYYRHCLLYLSCIDLDKCSHTELVERAVDLSVAAILGDIYNFGELLLHPVFELLVGTQHEWLHDLN"
  },
  {
    "reference": "MKIRTLSGSVLEPPSAVRATPGTSMLKLEPGGSTIPKIPFIRPSFPGPAELAEDFVQIAQANWYTNFGPNERRFARALRDYLGPHLHVATLANGTLALLAALHVSFGAGTRDRYLLMPSFTFVGVAQAALWTGYRPWFIDIDANTWQPCVHSARAVIERFRDRIAGILLANVFGVGNPQISVWEELAAEWELPIVLDSAAGFGSTYADGERLGGRGACEIFSFHATKPFAVGEGGALVSRDPRLVEHAYKFQNFGLVQTRESIQLGMNGKLSEISAAIGLRQLVGLDRRLASRRKVLECYRTGMADAGVRFQDNANVASLCFASACCTSADHKAAVLGSLRRHAIEARDYYNPPQHRHPYFVTNAELVESTDLAVTADICSRIVSLPVHDHMAPDDVARVVAAVQEAEVRGE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be able to regulate cellular response to iron ion starvation. The designed protein sequence is ",
    "response#1": "FHKAFRAQLAELQFLAGDTVRSGSDLAVELRSKFEFLKLVYKYHSAAEDEVIFSALDTRVKNIVFNYSLEHDATDDLFTSVFHWLNVLEEEQGNRADVLREVVLCIGTIQSSICQHMLKEERQVFPC",
    "response#2": "FHKAFRAQLAELQFLAGDTVRSGSDLAVELRSKFEFLKLVYKYHSAAEDEVIFSALDTRVKNIVFNYSLEHDATDDLFTSVFHWLNVLEEEQGNRADVLREVVLCIGTIQSSICQHMLKEERQVFPG",
    "response#3": "QFVKEVEKKIIEYDKDIAFNEAIILYEIAKHDADLVKNLASTINQHYFKNTIELCSIYPAKVGLCPQDCKFCSQSIHHSCLIEIKDLAALDEVIEYLEYVISFPIKRFCLVTSGEKLDDSEFEKILDIYSHISKNYNILLCASLGFLTQERAKKLLKVGVVKYHNNLETSSTYFKNICSTHTQQQKIETLKIAKEAGLQICSGGIISMGEDMIERIKLAFELRELDVDSVPINILNPIKGTPLEDIKIIDKNEIFITLALFRIVLPKKTILLAGGKENALGDMEKMAYECGVNGCMVGNYLTTRGMGIREKIEMLESLDLKFQTSW"
  },
  {
    "reference": "METGHVTAPIAAIILAAGKGTRMKSDMHKVLHPIAGRPMLLHLIDSVKALGAAREVVVVGAGREQVEAAVTPLGAETAHQAEQLGTGHAVLQAKEALAGFSGDVLILYGDVPLVTTATMRRMVERLHEDDAPAVVVLGFRPADPGAYGRLIVEGGDRLSKIVEFKDASPEERAVTLCNSGLMAVRGADLFALLARVTSDNAAGEYYLTDIVELANGDGRVAAVIETDATEVTGVNSRGELAALEAEWQRARRARAMVEGATLIAPETVWFAHDTQIGRDVTIEPNVVFGPGVTVADGVVLRAFSHIEGAVIASGATVGPFARLRPGAVLEANAHVGNFVELKNAVLGKGAKANHLTYLGDASVGAGANIGAGTITCNYNGFLKQKTVIGAGAFIGSNSALVAPVAIGEGAIIGAGSVITRDVEADALAVTRPETLKKQGWATTFRATMKARKAAK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein with glucosamine-1-phosphate N-acetyltransferase activity functions is required to enable lipid A biosynthetic process.2. The designed protein must have at least one MobA-like NTP transferase domain.3. The designed protein should interact with enzymes and substrates within the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, Nucleotide-sugar biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "VIILAAGKGTRMKSDLHKVLHPVAGRPMLLHLLGGFGELAPAATVVVVGDKRDQVEAALAGTGAVVAVQEPQLGTAHAALQGRDALAGFDGLVLVCFGDCPMLTAATVRRLCAPLDAGAMVAVLGFEPADAGRYGRLVVEGGALDRIVEHKDASEAERAITLCNSGVMAADCATLFDLVAKIGNDNAKGEYYLTDIVELARARGGVAKVVEGVTLRDPARFDVRGEVTVGRDVMIDINVILEGRVVIEDDVSIGPNCVIKNTTLRKGSIIKANSHLEGAIVGEGADAGPFARLRPGSVLGPKAHVGNFVELKNAELGEGAKAGHLSYLGDAVIGARTNIGAGTITCNYDGANKHKTVMGEDVFIGSNNSLVAPVDISSGATTAAGSTINQNVAAEQLAVARARDKVQLGLGLQDRLKEARKAQKK",
    "response#2": "VIILAAGKGTRMKSDLHKVLHPVAGRPMLLHLLGGFGELAPAATVVVVGDKRDQVEAALAGTGAVVAVQEPQLGTAHAALQGRDALAGFDGLVLVCFGDCPMLTAATVRRLCAPLDAGAMVAVLGFEAADPTGLGRILRDAEGTVQAIVEEKDATAEQLAITEINSGILAFDAAFLREALPRLSTDNAQGEKYLTDVIGVARADGGRVTALVAEDPWLVEGVNDRVQLATLGAELNRRIVTGHMRAGVEVGAGAMVGPFARLRAGAQLGADVHIGNFVEVKGRAELAAAEAQWQAFRREEAMAEGVSLKAPDTVWFSWDTVIGRDVTIEPNVFFGPGVTIADGATIRGFSHIEGATIASGCEVGPFARLRPGTVLEEKAKVGNFVEIKQAVLGKGAKANHLSYLGDASVGAGANIGAGTITCNYDGYFKYRTEIGDRAFIGSNSALVAPVKIGADAIVGAGSAVTRDVGDGELRLVRGEGAEVNAPRR",
    "response#3": "VIILAAGKGTRMKSDLHKVLHPVAGRPMLLHLLGGFGELAPAATVVVVGDKRDQVEAALAGTGAVVAVQEPQLGTAHAALQGRDALAGFDGLVLVCFGDCPMLTAATVRRLCAPLDAGAMVAVLGFAADPTGYGRLVREGGEVVAIREEKDASPEERAVREINSGTYVFRAGALREHLDRIGTANAQGEKYLTDVIGLARDAGARVGALVAADPDEALGCNDRVQLAEIGRRVLARTVERHMRA"
  },
  {
    "reference": "MRLAPQKPLLLSTVLHLLLSIWMLGFASLAGATVQEPLAASDTPKPVSAALFSSIERLSRLVDITYCVGNTGVWKPFACASRCNEFPTLTLERTWRTGILMSDSCGLIAVDHGTPRHDAGEGKDDPLAEKAIIVAFRGTYSLTNTIIDLSTIPQEYVPYPSPDDGGNEPPREPSHRCDNCTVHSGFLASWRHARKVVLPELKVLRQKYPEYPIRLVGHSLGGAVAMLAALEMRVSLGWRDTVVTTFGEPRVGNRQLCDYLNAVFELNLGDEMDPAEREYRRVTHADDPVPLLPPAEWGYSSHGGEFFISKKDLPPSVEDVLVCHGDHDENCIARGKSSAVSVPAGDYDDALSTLEEQDVMLADALPWIPARLKLWELFFAHRDYFWRLGLCVPGGDPANWGRKGGEGAAESISAEG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must have a Nucleophile, Charge relay system that is structurally stable and resistant to denaturation.2. For general function, the protein need meet that Active towards p-nitrophenol esters of various carbon chain length with preference for medium-chain fatty acids (C-8)3. The protein can be targeted to extracellular region and perform acetylxylan esterase activity. The designed protein sequence is ",
    "response#1": "VVGDSISAALGLDTSQGWVALLQKRLADEGYDYRVVNASISGDTSAGGLARLPALLAEEKPALVVIELGGNDGLRGMAPAQLQQNLASMAQKARAEGAKVLLLGIQLPPNYGPRYIEAFSRVYGAVAAQEKTALVPFFLEGVGGVQGMMQADGIHPW",
    "response#2": "VVGDSISAALGLDTSQGWVALLQKRLADEGYDYRVVNASISGDTSAGGLARLPALLAEEKPALVVIELGGNDGLRGMAPAQLQQNLASMAQKARAEGAKVLLLGIQLPPNYGPRYIEAFSRVYGAVAAQEKTALVPFFLEGVGGVQGMMQADGIHPR",
    "response#3": "VVGDSISAALGLDTSQGWVALLQKRLADEGYDYRVVNASISGDTSAGGLARLPALLAEEKPALVVIELGGNDGLRGMAPAQLQQNLASMAQKARAEGAKVLLLGIQLPPNYGPRYIEAFSRVYGAVAAQEKTALVPFFLEGVGGVQGMMQADGIHPL"
  },
  {
    "reference": "MRLKNRLFWIAAFIAFFLDQITKYWVVQTFSLGQTLPLLTGIFHFTYVTNTGAAFSLLSGKVEWLRWLSLGVSLVLIALALFGPTLNLWDQLGYGLILGGAMGNGIDRFVLGHVVDFLDFRLISFPVFNVADSFISIGIVFLLIASFQKTPTSTGRLD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be optimized for activity in Protein modification pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The desired protein should be able to perform Release of signal peptides from bacterial membrane   prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in   which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and   Zaa (Gly or Ala) have small, neutral side chains.3. The designed protein must be able to regulate proteolysis. The designed protein sequence is ",
    "response#1": "GW",
    "response#2": "GMVILAGWASEKLDDRLFEQARGRDVFMLHDGPPYANGHLHIGHALNKILKDVINRAHRMSGYGIHYVPGWDCHGLPIEWKIEEEYRKRGQDKDSVPVLQFRAECRAYAQKWLDTQASEFRRLGVQAEWANRYATMDFSSEAAIAGEIGKFLLNGLLYRGLRPVMWSPVEKTALAEAEIEYRDHESTAIHVAFPFVTDPTPAKALKDVAAVIWTTTPWTIPANRAIAYGPDITYAVVRVDGVGDNATLEPEARVLVAEDLIAPFCQAADVTAHHVLYTLPGSALEGAICAHPLRGQGYDFDVPLLPGAHVTTEAGTGLVHTAPAHGEDDFNLGRAYGLEITEMVMDDGRYADWVPLFAGGHVFKAAEPVCQALLEAMQRAVAAGMAPCGLVARAVLVHSYPHSWRSRAPIIYRATPQWFISMDGEGRLRERALAALEKVTFVPAQSRNRLTSMVAGRPDWCISRQRAWGVPIAVFVEKRTGEVLRDAAVMERIVTAFRAEGADAWYSSPAARFLGEGRNPDDYEQVFDIVDVWFESGSTHAFVLGHGDMPFPADLYLEGSDQHRGWFQSSLLESVGTRGVAPYKALVTNGFVLDEQGRKMSKSLGNVIAPQDVNDSLGADILRLWVMNSDTNDDLRIGKEILKQQGELYRRLRNTLRWLLGALDGFTEAERVPFAELPELERWVLHRMSELGSLVARAVETHEWVGVYPALHGFCAADLSAFYFDVRKDTIYCDGASSRVLR",
    "response#3": "IGKEILKQQGELYRRLRNTLRWLLGALDGFTEAERVPFAELPELERWVLHRMSELGSLVARAVETHEWVGVYPALHGFCAADLSAFYFDVRKDTIYCDGASS"
  },
  {
    "reference": "MNVALFISSACCPILNLCFGFCPTGNTVSNLIFLLPPIYGAIQTYKDGLEKRYIAAFLCLTAVGLGSWCFHMTLKYEMQLLDELPMIYSCCVFVYCLYECFKHKNTVNYPLLFVLITLSFIVSIIYLNLKEPVFHQIMYGSLVSIIVLRSVYIVLWVYPWLRGLGYTSLSVFLMGFFFWNVDNIFCDKLRELREKMPPVVGAVTQFHAWWHIFTGLGSYLHILLSLYTRTLFLKYRPKVKFVFGIWPILLVEPPKKH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design must be able to perform calcium ion binding, dihydroceramidase activity, zinc ion binding, phytoceramidase activity in order to contribute to sphingosine biosynthetic process, inflammatory response, positive regulation of cell population proliferation.2. The protein must exhibit the following characteristics: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid..3. Ideal protein should have a binding affinity for key enzymes in the Sphingolipid metabolism pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "LVYSSKESITESICTGKGVYQSRKHGLWRKGETSGAVQEVVSLRADCDSDTLEFSVIQHGTGFCHLGTSTCR",
    "response#2": "LVYSSKESITESICTGKGVYQSRKHGLWRKGETSGAVQEVVSLRADCDSDTLEFSVIQHGTGFCHLGTSTCR",
    "response#3": "TVQSSQVVAPNSEHDDGTMDMLLVHGCGRLRLLRFFILLQTGRHLSLPYVECVKVKSVKIKAGKNTHDSCGIDGELFALHGEVISTMLPEQCR"
  },
  {
    "reference": "CCSALCSRYHCLPCC",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Slowly and reversibly inhibits human nicotinic acetylcholine receptors (nAChR) alpha-7/CHRNA7 (IC(50)=45.7 uM) The designed protein sequence is ",
    "response#1": "EERLLKRLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPGDYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFYDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLVSMHSRVDQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFIIRRR",
    "response#2": "ETRLVAKLFEDYSSVVRPVEDHREIVQVTVGLQLIQLINVDEVNQIVTTNVRLKQQWVDYNLKWNPDDYGGVKKIHIPSEKIWRPDVVLYNNADGDFAIVKFTKVLLDYTGHITWTPPAIFKSYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINPESDQPDLSNFMESGEWVIKEARGWKHWVFYSCCPTTPYLDITYHFVMQRR",
    "response#3": "EERLLKKLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPADYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFHDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLVNMHSRVDQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFVIRRR"
  },
  {
    "reference": "MDYLPIFCRLDNKPVLLVGGGEVAERKARLLLDAGARLTVVSPALDPELAALATSGTIDWLAAEFEPAHLTGKWLVVAATDRREVNALVYQSANQAGIFANVVDDPKRSSFIMPSIIDRSPLMVAISSGGKAPVLARLLREKLEALLPQHLGAVAAFAGGLRARVKARFASMGERRQFWERLLSADRLGQALARGDKASANQLADTLFAEETGAKGEVILVGAGPGDPGLLTLHALRHMQQADLVVYDQLVSDEVMALVRRDARRIFVGKQAGNHCVPQEGINQLLLEEASKGQRVVRLKGGDPFIFGRGGEELETLVGSGVGFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFVTAHGKGGTQDLDWPLLARDQQTLVFYMGLSSCATIRQKLTAHGKAGTTPVALIERGTQLNQRVIRGTLDQLPELAVGVESPALIMVGSVVTLADKLAWFGQTNHGVQAAALA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a high specificity for S-adenosyl-L-methionine binding, with minimal off-target effects.2. For general function, the protein need meet that Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1; Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin; Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.3. A protein with NAD binding, sirohydrochlorin ferrochelatase activity is needed to enhance cobalamin biosynthetic process, siroheme biosynthetic process, methylation in a specific context.4. The protein design should take into account the physiological conditions of the Porphyrin-containing compound metabolism, siroheme biosynthesis, sirohydrochlorin from precorrin-2: step 1/1 pathway.5. The protein's Proton donor, Proton acceptor must be carefully engineered to ensure that the amino acids involved in 2 H(+) + siroheme = Fe(2+) + sirohydrochlorin are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "FPIFTKLENKPALIVGGGDVAHRKCQAMLQANASITLVAPEFCAELIALSNAKKVTLIHDYFTEQHVNNQSLVIAATDLEAVNKAVFDAAEAKNIFVNVVDDQPKCSFIFPSIVDRSPITIAISSAGKAPVLARRLREKLETIIPQHIGPLAQLAGNFRDKVKSQLDSFSKRRMFWEKAFTSDAVAQVAKGDITAAEQTLENIEAYFSNQPGPNEWGSVVLVGAGPGDPGLLTLRGLRALNEADVILHDHLASDEVLRLARRDAERIDVGKRAGGGGVPQHRIHALMIEHARAGKRVVRLKGGDPFVFGRGGEELQALRAQGIAYEVVPGVTAALACAAYAGIPLTHRKLAQSLRLVTAHHKDSLDEVDWRSLTQERQTLAVYMGVAMLETLQSQLIAHGRAADTPFALIENGSRAEQRVVVGPLSELAERARIHGVASPALLIVGEVAAVGAGPGDPGLITLNALRAMNEADVILHDRLVSDDVLQLSRRDAVRIEVGKSAQGHSVRQEQIHTLMLEHARAGKRVVRLKGGDPFVFGRGGEELEFLRAHGIPYEVVPGVTAALACAAYAGIPLTHRDHAQSLRLVTAHCKDSQDTLDWAALAQERQTLAVYMGVAGLDNFRARLLQAGRAPQTPFALIENGSRANQRVVTGTLADLPDTARCHQVRSPALLILGEVSTHEQRAALTVYLIGAGPGAPDLLTFRALRLMQQADVIVYDHLVSPEILDLARRDSEKIYVGKQRQNHSLPQDSINRLLADLAKAGKRVVRLKGGDPFIFGRGGEEIETLMQEGIAFQVVPGITAASGCASYAGIPLTHRDHAQSCTFVTGHLKDNTINLNWTQLAAPNQTIVVYMGSMGLEKICQSMISHGSPPDLPIALVQQGTTRTQRVFTGTLAT",
    "response#2": "LPIFLDVKQQPCLVVGGGAVALRKLTLLLNAGAAVRLIAPQVTPELRPLIDSGAVTHRPRYFETRDLDDVRLVIAATDAPATNADIATLARARGIPVNVADQPQLCSFIMPSVIDRSPVVVAVSTGAASPVLARMIRARLESLVPSSYGRLAELCARYRDRVKQRFDQQRERRRFWDRVLQGGVAERVFSGQMEQANAAIERELLAVGAGPGDPGLLTLKALRALNEADVILYDRLVSADILSLARRDAEREDVGKRPGEDHAATQARIHALMVEHARRGRRVVRLKGGDAFIFGRGGEELQHLRAHGVPYEVVPGVTAALACAAHAGIPLTHRDHAQSLHLITAHCRDDDDAVDWGALAGSRQTLAFYMGVSQLDALARRLQEHGRAADTPFALVENGSRPEQRVLTGRLAELPALARAHDIRAPALLIVGRVVGAGPGDPGLLTLKALRALNEADVILYDRLVSADILSLARRDAEREDVGKRPGEDHAATQARIHALMVEHARRGRRVVRLKGGDAFIFGRGGEELQHLRAHGVPYEVVPGVTAALACAAHAGIPLTHRDHAQSLHLITAHCRDDDDAVDWGALAGSRQTLAFYMGVSQLDALARRLQEHGRAADTPFALVENGSRPEQRVLTGRLAELPALARAHDIRAPALLIVGRVVGAGPGDPGLLTLRGLRVLNEADVILHDRLVSDEVLALARRDADRIELGKQVGGDHEATQARIHALLVEHARAGKRVVRLKGGDPFVFGRGGEELEVLHAHGIDFEVVPGITAAVACAAYAGVPLTHREHAQSVRFVTALGRDGTDPVDWPALARERQTLAFYMGVSGLTRLREQLIAHGRAPGTPFALVENGTRADQRVVTGTLDTLPEAAARHAVKAPALLILGEV",
    "response#3": "VGAGPGDPGLLTLRGLRALNEADVILHDHLASDEVLRLARRDAERIDVGKRAGGGGVPQHRIHALMIEHARAGKRVVRLKGGDPFVFGRGGEELQALRAQGIAYEVVPGVTAALACAAYAGIPLTHRKLAQSLRLVTAHHKDSLDEVDWRSLTQERQTLAVYMGVAMLETLQSQLIAHGRAADTPFALIENGSRAEQRVVVGPLSELAERARIHGVASPALLIVGEVEPGTVVVRADGVGSGPGEPDLLTFRALRLMQRADVVLYDSLIAPAILALVSPEAERIHVGKRAAEHTLPQDDINALMVALAREGKRVLRLKGGDPFVFGRGGEEISELAAAGIPFQIVPGITAANGCAAYAGIPLTHRDCAQSVTFVTGHLKAGALNLSWAELAQRGQTVVFFMGRRNLATICARLIEHGLPGDWPAAMIIDGTTARQRLIAATLDDLPDRVAAERSTGAALLIVGEVEPGTVVVRADGSATEVVRLKGGDPFIFGRGGEEIETLMQEGIAFQVVPGPDGADATSVQASGRGSVVLVGAGPGDPGLLTLKALRALNEADVILYDRLVSADILSLARRDAEREDVGKRPGEDHAATQARIHALMVEHARRGRRVVRLKGGDAFIFGRGGEELQHLRAHGVPYEVVPGVTAALACAAHAGIPLTHRDHAQSLHLITAHCRDDDDAVDWGALAGSRQTLAFYMGVSQLDALARRLQEHGRAADTPFALVENGSRPEQRVLTGRLAELPALARAHDIRAPALLIVGRVAALRGGADTVRPLARSA"
  },
  {
    "reference": "MLSQCFRSSLAKHCFVAKNQALHGKFVNSIMCLSSSSSNSGSSNSNSNSGSSNSNIRYNDNYRSNDDGTNFEELQLPSALKSETESLNGKGKNKLSKRPQTLLEALIESHGTLPEEVDEPKITRNVASESATSATSATSATSATSATSATSATSATSATSATSATSTTTIITTTATVPVENNVSTPYWVKRELTMKAKHSQWNPKKKLSREDMLKLRELKENFPHYKTVELAELFRVSPEAVRRILKSNWVPNDIDEDRIIARREKQKQKRQQEIQLKKLEFISNRGKKNVSSRPQQRQQQQPKQQQRQRSKVGYKNGFAFNEKYGGLGNKF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should emphasize Polar residues compositional bias.2. The designed protein localizes to the mitochondrion.3. For general function, the protein need meet that Required for respiratory activity and maintenance and expression of the mitochondrial genome. The designed protein sequence is ",
    "response#1": "LNVNNTKYWTDYNKLIYNPKSLTTVNNFVHNENNHESGYHYNFNSLKYDSFNIGQEEFKACNNGNGGYGYDDNDNNKSIENFRYFLEKTDRLQGLQLLTNLNDAWGGFTSEMLIDLIDEFFNNTSSDKQNLWIYGIMNSTKLSEKTQSIRTKLSFIKTLIELTKQSSLIFPMNLNNSKDESWHNNYSMLTDKYNSGSNWHQSSLYATFINSC",
    "response#2": "LNVNNTKYWTDYNKLIYNPKSLTTVNNFVHNENNHESGYHYNFNSLKYDSFNIGQEEFKACNNGNGGYGYDDNDNNKSIENFRYFLEKTDRLQGLQLLTNLNDAWGGFTSEMLIDLIDEFFNNTSSDKQNLWIYGIMNSTKLSEKTQSIRTKLSFIKTLIELTKQSSLIFPMNLNNSKDESWHNNYSMLTDKYNSGSNWHQSSLYATFINSC",
    "response#3": "LNVNNTKYWTDYNKLIYNPKSLTTVNNFVHNENNHESGYHYNFNSLKYDSFNIGQEEFKACNNGNGGYGYDDNDNNKSIENFRYFLEKTDRLQGLQLLTNLNDAWGGFTSEMLIDLIDEFFNNTSSDKQNLWIYGIMNSTKLSEKTQSIRTKLSFIKTLIELTKQSSLIFPMNLNNSKDESWHNNYSMLTDKYNSGSNWHQSSLYATFINSC"
  },
  {
    "reference": "MNLGALVSETRNPATMGLDEMSTLEMVSCFNQEDRKVPEAIEKVLPAIAQAVDLAAAALKAGGRLIYLGAGTSGRLGVLDASECPPTFGVPHGMVIGLIAGGPGALLKAVEGAEDDAALGEADLVALDLTATDMVVGLAASGRTPYVIGALRYARQLGCPTAAISCNPDSPIAHEVQVAISPVVGPEALTGSTRLKSGTAQKLVLNMLSTGAMVKLGKVYENLMVDVKATNVKLVDRACRIVVEATGAERSQAEAALTQTGFEVKPAILMILAGIDAQEAQQRLQQHDGYLRAALTR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should interact with enzymes and substrates within the peptidoglycan recycling pathway to enhance or regulate its activity.2. For general function, the protein need meet that Together with AnmK, is also required for the utilization of anhydro-N-acetylmuramic acid (anhMurNAc) either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling.; Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D- lactate3. The protein with the reaction H2O + N-acetyl-D-muramate 6-phosphate = (R)-lactate + N-   acetyl-D-glucosamine 6-phosphate, the active site, Proton donor must be optimized to ensure efficient catalysis.4. The designed protein must possess carbohydrate derivative binding, carbon-oxygen lyase activity to support N-acetylmuramic acid catabolic process, amino sugar catabolic process, 1,6-anhydro-N-acetyl-beta-muramic acid catabolic process, carbohydrate metabolic process under certain conditions.5. The designed protein have SIS domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MSATISIKNGGRLIYIGAGTSGRLGILDAVECRPTFSVPDELVVGVIAGGEKAIQHAVEGAEDDVDAGRADLVALSLNHNDTVIGISASGRTPYVSGALEYARSLGCFTGAIACSPNAAIFTHADVAICPVVGPEALTGSTRMKSGTAQKLILNMISTSTMIKLGRRGVVAGPEALTGSTRMKSGTAQMAVAQAVDLAADALKQGGRLIYLGAGTSGRLGVLDASECPPTFGVPHGMVIGLIAGGPGALLKAVEGAEDDIALGMRDLQDLQLTATDMVVGLAASGRTPYVIGALRYFGHPTDKLPVIGVGAEVLTGSSRMKSGTAQVETADCLKSGGRLIYMGAGTSGRLGVLDASECPPTFGIPHDCVIGLIAGGSGAMFKAVEGAEDDPQLGGSDLAALSLSSADMVIGLAASGRTPYVIGGLRYARRLGCRTAAISCNPLSPIAQEAEFAISPVVGPEVLTGSTRLKSGTAQKLILNMISTGAMVRMGGAPARVVLADPALAGGLPIASAIDQIVARMRQGGRLIYLGAGTSGRLGVLDASECPPTFNTTPERVVGLIAGGETALTTAVEGAEDDAEAGAQELAALDVTALDSVVGIASSGRTPYVLGGLPAAGLVPDAVRVAAPPAYSMNLDSLSPLELVTLAREVGVPADAIGAGLRDARVPA",
    "response#2": "MNLATISIKNGGRLIYIGAGTSGRLGILDAVECRPTFSVPDELVVGVIAGGEKAIQHAVEGAEDDVDAGRADLVALSLNHNDTVIGISASGRTPYVSGALEYARSLGCFTGAIACSPNAAIFTHADVAICPVVGPEALTGSTRMKSGTAQKLILNMISTSTMIKLGQGCGVMTNRFENPSGYGRVFCDGNNLVKEIIEDRDCTQAQREIKEVNTGIYCFKADWLFKNLPRIKPNNNQQEFLITDTFNYLLKEGDTIAVIKTEDPLQIEGVNDRVQLAEAEKILRQRINDFWLLNGLSVQDPASTYIDARCEISGNVTILSHCHIGPDVEIGENSIIGPFAHLRPGTKLKKGTKIGNFVEIKNASIGENSKAKHLSYIGDAEIGKNVNIGAGTITCNYDGVRKHQTIIEDGVFIGSDTMIVAPLTVKKGALTGGTLVAKDVKPRSVVVGVPGRVIKQG",
    "response#3": "MPATISIKNGGRLIYIGAGTSGRLGILDAVECRPTFSVPDELVVGVIAGGEKAIQHAVEGAEDDVDAGRADLVALSLNHNDTVIGISASGRTPYVSGALEYARSLGCFTGAIACSPNAAIFTHADVAICPVVGPEALTGSTRMKSGTAQKLILNMISTSTMIKLGSRNAGANPKKIVAGPEALTGSTRMKSGTAQFAFNVACDVAEKIFGSMEKRLKISMNLDSLSPLELVTLAVTSGVPFANAIKALESIEGAKCRMEVIGLKNGETIHFGPEVVTGSTRLKAGTAQEAIVRTFQQGGRLVYIGAGTSGRLGVLDASECPPTYGVKPEMVVGLIAGGERALRHPIEGAEDNAEQGKADLQQINFSKKDILVGIAASGRTPYVIGALNYAKSLGAITISIASNPDSAMASIADIAIDTLVGAEVLTGSSRMKSGTAQKLVLNMLTTASMVLMGLGPGASAAAASALHGVKQIAQAVEKIVQAFQNGGRLVYIGAGTSGRLGVLDASECPPTYGVSSEMVVGIIAGGERALRHPIEGAEDNTEQGKADLQAVNFSQKDVLVGIAASGRTPYVIGALEYGQHVVLPI"
  },
  {
    "reference": "MSTEPAPLLALDLTGTFAFGLNGALTAVRAARLDVVGVVVLGMITALGGGVIRDVLIDSLPPAAFLDWRYYTLAAAGGLLAFAVSRHLRRLEPAITVLDAVGLSTFAVIGASKALDAGLAVVPAMLLGVITAVGGGTIRDTLVGRIPTVLRTGLYAIPALAGAAVTVATTETGVYGLPAALGAAAVCFLIRMLGLHFGINAPEPPETRPSGGGTRRQK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "ARQPELMNALQTKMFLLAGLIF",
    "response#2": "GQSS",
    "response#3": "MTARQPELMNALQTKMFLLAGLIL"
  },
  {
    "reference": "MSLPEKAFPVSWDQFHRDARALAWRLAGSKGRWKAIVCITRGGLVPAAIISRELGIRVIETVSIASYHDYTEQGELKVLKEISPALLENEGEEVLIVDDLTDTGKTAGLVRAMMPKAHFATVYAKPKGCPLVDTFVTEVSQDTWIYFPWDMGFTYQQPISEGHRG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be involved in XMP salvage by localizing to plasma membrane and exhibiting hypoxanthine phosphoribosyltransferase activity, guanine phosphoribosyltransferase activity.2. The protein contains novel Phosphoribosyltransferase domains that confer a unique function or activity.3. The Mg(2+), xanthine binding site should be stable and able to withstand changes in environmental conditions.4. The protein design should take into account the physiological conditions of the Purine metabolism, XMP from xanthine: step 1/1, XMP biosynthesis via salvage pathway pathway. The designed protein sequence is ",
    "response#1": "SWDQIHRDARALSWRLDGKGPGAGGQWRAVVAITRGGLVPAMIVARELDIRVIDTISVKSYNHQAQASAVVTKSPQPELMEDGAGILIVDDLVDSGKTLELVRRLYPKAHFATVYAKPHGKPQVDTYVTEVSQDTWIFFPWDMALQY",
    "response#2": "SWEELHRNGRALAWRLMEKSPFKGVIAVTRGGMVPAAIVARELDIRHIDTVCIAAYRDDKSVGEVNLIKGLELPDDGDGWVIVDDLVDSGITARHVRKMVPKAHFATIYAKPKGLDQVDTFITEVSQDTWILFPWR",
    "response#3": "GPFEAIVCITRGGLVPAAIVARELNVRIIETVCIASYHDYKNQGDIKVMKGVGPEITSMRGQGKGVLIVDDLVDTGKTARVVREILPAAHFATVYAKPMGL"
  },
  {
    "reference": "MRKPYRKVVVGGTFDRLHLGHKALLRKAFEVGRYVYVGLTSDEMIRNKPYAEKILPYELRLMDLLKFFEVNGYTNYRIIKINTAIGFADRIKSLEAIVVSEETYKGALLVNRAREERGLKPLEIVTIKLVKSRIGPKISSTLIRAGLIDPFGNPLKKDN",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Reversibly transfers an adenylyl group from ATP to 4'- phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate..2. The protein should be optimized for activity in Cofactor biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. A protein that contributes to coenzyme A biosynthetic process by functioning in cytoplasm with pantetheine-phosphate adenylyltransferase activity. The designed protein sequence is ",
    "response#1": "GSDNPVKVNAVLSVMSKIFSSLEVGCTEVETSISEQPFGERTRQGAVERAKAALSTADFGVGLEAGVFELPDGLYDIQYCAIIDKCGHVTIGHGSGFKYPSEVARRVREGYSVGQAFEDIYGEEDVGRREGAIGYLTNGLMDRTSLSEQAVLAALVPRA",
    "response#2": "GSDNPVKVNAVLSVMSKIFSSLEVGCTEVETSISEQPFGERTRQGAVERAKAALSTADFGVGLEAGVFELPDGLYDIQYCAIIDKCGHVTIGHGSGFKYPSEVARRVREGYSVGQAFEDIYGEEDVGRREGAIGYLTNGLMDRTSLSEQAVLAALVPRA",
    "response#3": "VYPGSFDPITNGHLDIIKRASQVFDKVVVGILVNPDKKGLFSIEEREKLISRVVKDMPNVEVRSFSGLLVDFMKEENIKVIIKGLRAMSDFEYEFQMALMNKKLNPDIETLFMMTCAQYSYLSSSSIKG"
  },
  {
    "reference": "MRKLIEFQILDRNAKHYGLDLFELMKNAGTLVAKHVKESVDSSMPVLFLCGHGNNGGDGYIAADLLQKDGFDVKLYSVKRPVTEISKRALDNYSHKIYNIKEFDFSEAEPVLVVDCLLGSGIKGELRTPYAELINKVNKFENILSVDVPSGFGTLNAVKPSQTITFHEKKLGMDEMSCGEVFVVDVGFSSDIDEFTGPGELLLFPDFDPKKYKGQNGKVAVIGGGEFSGAPSLAGIGAYRGGADLVHLFVPESSYEQVASFAPELIVHKLKGNIINKEIFTIFEKENFDCVVIGPGMGKHESSIEAVQMLIDDFENIVIDADAIKRYHFRESNILLTPHLGELSRLRIENDRISLMSFAKSNNLSLLVKGEXDYVTDGTYFKINSTGHPRMAVGGTGDLLSGLXGXLMAKGLSPFEAGRLAAYALGKAGELCYKEYGASFLPTDLGLCISKILSS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein requires Mg(2+) to activate a critical catalytic site.2. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. The designed protein sequence is ",
    "response#1": "RASLPTRRPSSHKGTFGHAGIIAGSVGKTGAAAMAARAALRIGAGLVTVAVPSSVNDVLEAKLLEAMTIPMPETKSRTFARTALDRLTAFIAARTAIAIGPGLSTHPETVELVQALTKQLDRPAVLDADALNALAGRASMLTACKTPPILTPHPGEMARLETDATPQSVNADRINTAARFARERGVFVVLKGARTVVARPDGTAAICPTGNPGMATAGTGDVLTGMIVGLLAQGLSSWEAASVATYLHGCAGDLAAAEKGQAGLIAGDLIEQIPFAVRR",
    "response#2": "LLIVGGSAEYSGAPALSALAALRTGVDMAVVAAPKAAADAIRGFSPNLIVRSLSGEHLARDDLPRLYELAAQSDGIVVGPGLGLEAETREAVIEFVKMARGPILVDADAIKALSAKPASLKGKRSVVTPHAGEFAILTGRAMADPPNIEERMRQVKDASKNLGTTILLKAHEDIISDGERLKLNLTGNPGMTVGGTGDVLSGIAGAFLAWGAEPFRAACAAAFVSGKAGDLAKSEKGYHIVATDVVEKG",
    "response#3": "LIVGGSFSMPGAVSMTASACIYSGAGLLTTALPASILSIVSNSVPETMFLPLPEKNGYIAGEVMGTDFTFGHYDAVAAGPGLGRERGGKQLIRKLLAEVKTPLLFDADALYYLPELKQEMKQREEPVIVTPHAGEMARLADVSIQEVNKHRFSISKEFARAYNCYVVLKGPYTIVTSPEGEQSINPTGNEALARGGTGDILTGIALGFMLQHRDVFSALCNAVYIHGLTADLALQKGFTATSMASSDLIHQLN"
  },
  {
    "reference": "MAAPAPRARTGGKKKDERRGFKGYKWEFRDSNKDFWAQGHAECKSLIMILLIAAMVCFQRVATHPIVILLLTMELSICAFFFFLYSLAINRYIPFVFWPMMDLMNDLACSTFLIGGIFFALEARRELPVPYLTGMILMGVTAFISIIDLCLQRRQFKSRKLRKFILLTPDRKGKKQDPKLLLMLAAKEDEEERQRELAEKAKRESMDPGW",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. The presence of MARVEL domains is critical for the interaction of the designed protein with its intended targets.3. The protein should be optimized for membrane-specific folding and stability, while maintaining cytokine activity. The designed protein sequence is ",
    "response#1": "YLCTGRACWQMLKALLNLLILACSSVSYNSTGGYTGITSLGGIYYYQYGGAYSGFDGADGERAQQLDVQFYQLKLPTVTAAMAYSGALMTFSCLTLLAGALRVPWHCPLWLVIEGLMDALIAGAYVPGLYFFFQHLSAAYSSDVCKERETLYQSKGYSGFNCGVHGGDIGAGVFAAMAIGVFAVGAVLFARM",
    "response#2": "YLCTGRACWQMLKALLNLLILACSSVSYNSTGGYTGITSLGGIYYYQYGGAYSGFDGADGERAQQLDVQFYQLKLPTVTAAMAYSGALMTFSCLTLLAGALRVPWHCPLWLVIEGLMDALIAGAYVPGLYFFFQHLSAAYSSDVCKERETLYQSKGYSGFNCGVHGGDIGAGVFAAMAIGVFAVGAVL",
    "response#3": "MATVSGLLKIVQAFVACIIFGALVHESRYGRYVATQWCVAVYSLCFMATVAVVVLSVMGHTGGLGCPFDRLVVVYTFLAVLLYLSAAVIWPVFCFDPKYGEPGRPSDCPRGSCPWDSQLVVAIFTYVNLLLYIVDLV"
  },
  {
    "reference": "MAADYWSSTQHQSWLFGREELAEARKVLGDAERPFIQQYPLPDLRLFNIYVNQQLIKLAKRLNVRQQALATAQVYVKRFYTKVEIRRTNPYLVLTTAFYLACKIEECPQHIRLVLGEARGLWPEFIAPDSAKIGECEFWLISEMNSQLIVHHPYRTLSELQSYLSLTSDEIALAWSVINDHYLTDLLLLHPPHVISVMAIFIAVVFKPNQHQVVSISGGSSATGALRDGSTNILSAFNDKTGAGMPAKVQRIVDWLANSDINIEAVIECTQDIVSLYEVWEQYSEKVCKEQIGRYVKSRGLDK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must have at least one Cyclin N-terminal domain.2. The designed protein should be optimized for cyclin-dependent protein serine/threonine kinase regulator activity activity in order to improve regulation of transcription by RNA polymerase II.3. The protein must exhibit the following characteristics: The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II .; Component of the SRB8-11 complex. The designed protein sequence is ",
    "response#1": "KAETTMYYAIKKFKTEKDGIEQLHYTGISQSACREMALCRELDNNHLTKLVEIFLQKKSIYMVYEFAEHDLLQIIHFHSHPEKRMIPPRMIRSIMWQILDGVSYLHQNWVLHRDLKPANIMVTMDGVVKIGDLGLARKFSNMLQTMYTGDKVVVTIWYRAPELLLGARHYTPAIDLWAVGCIFAELIGLQPIFKGEEAKMDSKKTVPFQANQLQRILKILGTPTPKSWPHLQKYPEYEQLSKFPKYRDNLPGWFHSAGGRDKHALSLLYHLLNYNPIERIDAINALDHSYL",
    "response#2": "QQLIKLGKRMSTRQQALATAQVYIKRFYTKNEIRHTNPYLVLTTAFYLACKMEECPQHIRFVVGEARSLWPEFITPDVSKLGECEFSLIY",
    "response#3": "KHPHKIIVMYLQVLECEKNQMLVQTAWNYMNDALRTSAFVRFEPETIACACIYLAARVLQIPLPSKPHWFLLFGATKEDIKEICINTMKLYSREKPHSEQLERQVEKRKIFLEEARLKAV"
  },
  {
    "reference": "STPSRLRGPGNGTGPRFDRDAVLGGGGGGGGGGSGSEGDSDSGEDEEPLSDRRGVKRALALAEAELGAAGQAAAGQEAPGSAASVAGTPGGGGGGYSGGTASGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRSDPTMDQRMSATGFEETDLTYQVSESDSSGETKDSLKPAFTVTNLPGTTSTIQTAPTTPTSMQVSSGPSFPITNYLAPVSASISPNAVTSANGTVLKTTGSSAVASGGLMQIPTGFTLMSGGAVAQQVPVQAIQVHQAPQQTSPTSDSSTDLTQTSSSGTVTLPATIMTSSVPTTVGGHMMYPSSHAVMYAPTSGLTDGGLAVLNAFSQAPSAMQVSHGQVQDQGGVSQVFLTAPSGTVQIPVSAVQLHQMAVIGQQSSSSSNLTELQVVNLDTSHSSKSD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that can perform protein homodimerization activity in chromatin to support positive regulation of transcription by RNA polymerase II. The designed protein sequence is ",
    "response#1": "FGVQETAIISQEVHNFIMDQGWSEYQFCNQIWAGKCPKTIRMFYSNLYKKLSHRDAKSIYHHVRRAYG",
    "response#2": "FGVQETAIISQEVHNFIMDQGWSEYQFCNQIWAGKCPKTIRMFYSNLYKKLSHRDAKSIYHHVRRAYS",
    "response#3": "ERKKTKKEYNTTILDFHWLSSSKPVIVSQFCAFDSSINMYRNKAQQLSPHGIFHPPFMKYAGLAIRRNGQLDFWYQFSNSKDHKKITLQLFNPHNERSKGLDFLQYAKITSVNNDNSILITTYSRLTGKLSFYKLFVDWNVNTAKPVVLNDPSLKIKHILDATVDQTDDEGRILDFTHVHVLAKVVAEKDAAPEVLLAYDVVGTPESLIKRFKLGQVRLPLDYLGILKPELNTSNENHNQALRSNRSNLRFLGVLNLHHKVASISSEVLDGFVSFYYRNGEIEIYNQNDWKLETERLLNQGPQGKFSNIITSILSAGFKYPTC"
  },
  {
    "reference": "MKKTLIIGVTGGSASGKTSVSHAILETFSNERIAMIEHDSYYKDQSHLTFEERTKTNYDHPLAFDTDYLIAQLKELQYGRAVDIPIYDYAKHTRSQETYRQEPVDVLIVEGILVLEDERLRDLMDIKIFVDTDDDVRIIRRIRRDIEERGRTLDSVITQYLEAVKPMYHQFIEPTKRYADVIIPEGVSNTVGVDIITTKIASILND",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design must be focused on enhancing the efficiency of CTP salvage. The designed protein sequence is ",
    "response#1": "GPFLWMGCFQRADEDTHKFKRGHLTVFSGPSHATGAARMAAMAGFRAGAGIVTVAAPQDAIGVLSATLTAVMVQGIESEDELSAWRDDTRHGTYILGPGFSDIERARRFVSLLKDKALVLDADGITAFRHDPQQLFDLFATDAPRILTPHEGEFGRLFPDIAADETLSKIEKAQAAAELSNAVIVYKGADTVIAAPDGRATVNTNAPPHLATAGSGDVLAGIAGGFLAQQVPAFEAAAAAVWLHGQTAQRLGPGLTAEDLAAAVRPFSV",
    "response#2": "VKIAVVGKYVALPDAYMSVTEALHHGGIEHDAQVKITWINAEELEEPNADLDEIFVGCKGILVPGGFGDRGVEGKIRAIQYAREHEIPFLGLCLGMQCAVIEFARHVAGLTNAHSTEFVPETPHPVIALMEDQQDVEEKGGTMRLGAYPCLLADGSHSRAEYGAGEISERHRHRFEFNNAYRAQLEEKGMVIAGTSPDDRLVEVVEIADHPWFVASQFHPELKSRPNHPHPLFAGFV",
    "response#3": "GPFLWMGCFQRADEDTHKFKRGHLTVFSGPSHATGAARMAAMAGFRAGAGIVTVAAPQDAIGVLSATLTAVMVQGIESEDELSAWRDDTRHGTYILGPGFSDIERARRFVSLLKDKALVLDADGITAFRHDPQQLFDLFATDAPRILTPHEGEFGRLFPDIAADETLSKIEKAQAAAELSNAVIVYKGADTVIAAPDGRATVNTNAPPHLATAGSGDVLAGIAGGFLAQQVPAFEAAAAAVWLHGQTAQRLGPGLTAEDLAAAVRPFSV"
  },
  {
    "reference": "MPIPAPHGGVLQDLVIRDAAIKPKLLSEIASENLTAVKLTERQLCDLELILNGGFSPLTGFLNEDDYNSVVEDMRLSSVKDADSGKGLLWSMPITLDVSAEFARSVSNGQKLVLQDLRDDKPLAVLTVESVYKPNKANEAKKVFRGDPEHPAVKYLNETAGEYYIGGSIQGLDYPTHYDYVSLRKTPTELRKEFENLGWDLQKIVAFQTRNPMHRAHRELTVRAAKDLGDDGHILVHPVVGLTKPGDIDHHTRVKVYQQILKKYPEGLATISLLPLAMRMGGDREALWHALIRMNYGVDHFIVGRDHAGPGKNSAGVDFYGPYDAQELLALVEAELAPKMKIVPFRMVTYLPDEDRYAPIDTIDLKTVKTANISGTELRLRLRNGTEIPEWFSYPEVVRILRESYPPRSQQGFAVVIDSTLYSDYSEHVAFALQSVLNQFTGGRRVTKLDEFADLFVVNELVQAGSGVILTQKAGADAVVKAVGADNVVVVSSQGPDAPYRFELKNGDVAALLAEIVGYLQEQGFYSQ",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein have Sulphate adenylyltransferase catalytic domains with specific sequence motifs that are crucial for its activity.2. The protein should have a high affinity for sulfate.3. We require a protein that can specifically bind to the metabolites produced in the Sulfur metabolism pathway.4. The protein must exhibit the following characteristics: Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids..5. The protein should be designed to localize specifically to cytoplasm in order to perform its role in hydrogen sulfide biosynthetic process, cysteine biosynthetic process, sulfate assimilation more effectively.6. The active site active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "APHGGILKDLYLRDAHKQKALWAEAQSLPSVVLTDRQLCDLELLLNGGFSPLEGFLNESNYNSSVSVVEHMRLANGLLWPIPITLDVSKEELNKSGIQPSKRIALLDSRDYEPLAILTIEDIYRPNKPKEAKSVYGADDIAHPAVNYLHNIAKEFNIGGSLEAIVTPEGPAYRYTPTELRAHFKKLAWRKVVAFQTRNPMHRAHRELTVRAARQRQANVLVHPVVGLTKPGDVDHYTRVRVYQAIMAKYPNGMGQLALLPLAMRMAGPREAVWHAIIRKNYGATHFIVGRDHAGPGKNSQGVDFYGPYDAQELVSKFKDELQIEMVPFQMMTYVPSSDDYQPTDEIAAGVQTLDISGTELRRRLKSGAPIPDWFSYDAVVKTLRQPHGGVLKDLIARDAPRRAQLFEESEKLPALILTERQLCDLELILNGGFSPLEGFMNEKDYNGVVENVRLADGTLFSMPITLDVSQEQIDELSIKPGARITLRDFRDDRHLAIITVEDVYRPDKVKEAKEVLGGDEEHPAIRYLYNTAKEFYVGGKLEAING",
    "response#2": "APHGGILKDLYLRDAHKQKALWAEAQSLPSVVLTDRQLCDLELLLNGGFSPLEGFLNESNYNSSVSVVEHMRLANGLLWPIPITLDVSKEELNKSGIQPSKRIALLDSRDYEPLAILTIEDIYRPNKPKEAKSVYGADDIAHPAVNYLHNIAKEFNIGGSLEAIRNTPAELRAWFKKLNWTRVVAFQTRNPMHRAHRELTVRAARSRQCNVLIHPVVGLTKPGDIDHYTRVRVYKALLPKYPQGMATLSLLPLAMRMGGPREAVWHAIIRKNHGCTHFIVGRDHAGPGKNSQGVDFYGPYDAQEMVEKYKNELDIEVVPFQMVAYLPETDEYVPEDEVPKGTKTLNISGTELRRRLRAGAPIPDWFTYPEVQLILRTPHGGVLKDLFARDLPRHAELRDESEKLPALVLTERHLCDLELILNGGFSPLEGFMIEKDYNGVVDNNRLADGALFSMPITLDVDQGQLDHLGIKAGARITLRDSRDDRNLAILAVEDVYRPDNTSNRVKEAKEVFGSDDDTHPGVKHLFNTAKEFYVGGKLEAINPPHGGILKDLVARDTPRRVELETEAETLASITLTERQLCDLELILNGGFSPLEGFMTEEDYNGVVTDCRLADGNLFSMPITLDISEETIKELDIKSGSRVALRDLRDDRNLAIITVKDIYRPDKVKEAKEVFGGDPEHPAIKYLNTQVKELYIGGNLEAVAPHGGVLKDLVARDAALHSTLVEEARELGDIFLTERQLCDLELILNGGFSPLEGFMTEDDYVSVRDTLRLVPVNGQRQGALFSMPITLDVSQEDIDRLGLQAGARVSLRDPRDDAALAILTVSDIYKPNKALEAEKVMGADDIAHPAVAYLHNQVKEFYVGGKVQAI",
    "response#3": "TPHGGVLKDLFARDLPRHAELRDESEKLPALVLTERHLCDLELILNGGFSPLEGFMIEKDYNGVVDNNRLADGALFSMPITLDVDQGQLDHLGIKAGARITLRDSRDDRNLAILAVEDVYRPDNTSNRVKEAKEVFGSDDDTHPGVKHLFNTAKEFYVGGKLEAINV"
  },
  {
    "reference": "MRTVLVALLALVALTQAISPLDLIKEEWHTYKLQHRKNYANEVEERFRMKIFNENRHKIAKHNQLFAQGKVSYKLGLNKYADMLHHEFKETMNGYNHTLRQLMRERTGLVGATYIPPAHVTVPKSVDWREHGAVTGVKDQGHCGSCWAFSSTGALEGQHFRKAGVLVSLSEQNLVDCSTKYGNNGCNGGLMDNAFRYIKDNGGIDTEKSYPYEGIDDSCHFNKATIGATDTGFVDIPEGDEEKMKKAVATMGPVSVAIDASHESFQLYSEGVYNEPECDEQNLDHGVLVVGYGTDESGMDYWLVKNSWGTTWGEQGYIKMARNQNNQCGIATASSYPTV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Important for the overall degradation of proteins in lysosomes. The designed protein sequence is ",
    "response#1": "PVTGNYSLKTGNKTCLLATVGLQLNISQDKPLLINIDPKTTHADGTCGNTSATLKLNDGNRTLIDFTFIVNASASVQKFYLREVNVTLLNYQNGSVILSADNNNLSKWDASLGNSYMCRKEQTLEINENLQVHTFNLWVQPFLVC",
    "response#2": "PVTGNYSLKTGNKTCLLATVGLQLNISQDKPLLINIDPKTTHADGTCGNTSATLKLNDGNRTLIDFTFIVNASASVQKFYLREVNVTLLNYQNGSVILSADNNNLSKWDASLGNSYMCRKEQTLEINENLQVHTFNLWVQPFLVD",
    "response#3": "SVVAINASGWRSNGEVMGLQIKDGVLYKDYDAAGYTGAEACVFFDDGTMKVYGNREVDADILISKGARNSFAFGIWLVKDSKPRTAQMTTWADLNVKHPRQAIGQRSDGTLVIITVDGRSLRSSGITAYDMPSLFLSEGCINAFLLDGGGSSQTAVEGKYINNISDGIERAVVDTLTI"
  },
  {
    "reference": "MEPIDPKLEPWNHPGSQPETPCTKCYCKICSYHCLVCFQTKGLGISYGRKKRGQRRSAPPSSEDHQNPISKQPLSQARGDPTGSKESKKEVESKTKADPFD",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: In the absence of Tat, the RNA Pol II generates short or non-processive transcripts that terminate at approximately 60 bp from the initiation site; Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication; Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation; In the cytoplasm, Tat is thought to act as a translational activator of HIV-1 mRNAs.; The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive; Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to promote processive transcription elongation by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts.2. The designed protein should have RNA-binding transcription regulator activity, metal ion binding, trans-activation response element binding, cyclin binding, actinin binding within host cell nucleolus to regulate modulation by virus of host chromatin organization, positive regulation of transcription elongation by RNA polymerase II, suppression by virus of host translation initiation, positive regulation of viral transcription.3. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding. The designed protein sequence is ",
    "response#1": "MEPVDPSLEPWKHPGSQPKTACTKCYCKVCCYHCQVCFITKGLGISYGRKKRRQRRRAPQGSQTHQVPLPKQPTSQPRGDPTGPKESKKKVERETETGTDPNQ",
    "response#2": "MEPVDPSLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFTKKALGISYGRKKRRQRRRPPQDSQTHQVSLSKQPASQPRGDPTGPKESKKKVEREAEAHPVD",
    "response#3": "MEPVDPNLEPWNHPGSQPTTPCSKCYCKKCCWHCQICFLKKGLGISYGRKKRKHRRGTPHSSKDHQNPIPKQPLPTTRGNPTGPKESKKEVESKAETDP"
  },
  {
    "reference": "MITLKDILYKVTLEKIVGSTNVAFTNIHFDSRAISLNDVFVAIKGTMADGHDFIENAANQGAIAIICEQLPKNIVNGITYVQVANSQHALAIMAANFYENPSENLKLIGITGTNGKTTISTLLYNLFKDAGFEVGLLSTVKILVGSQEYKATHTTPDSLTINRYLKEMVDAGVEYCFMEVSSHGIDQKRTEGLEFNGGVFTNLSHDHLDYHKDFKEYRDVKKKFFDGLSKKAFALTNIDDKNGLYMLQNCHAKKYTYALKSHADYKAQILENQFSGLLLKINNNELWVKLIGTFNAYNLLAIYATSELLGLEAIEILQLMSTLESVDGRFQFSISEENITAIVDYAHTPDALKNVLETINTIRTGNEELITVVGCGGDRDAEKRPKMGHIASQLSTKVIFTSDNPRTEDPDDIIQQIENGVSPENFKKTLSITDRKQAIKTACQMAQNNDIILVAGKGHETYQEINGKRTDFDDFKIVNELLTALKK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.2. The UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding site should be stable and able to withstand changes in environmental conditions.3. The designed protein should be optimized for UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, magnesium ion binding, ATP binding activity in order to improve cell cycle, regulation of cell shape.4. We are looking for a protein with broad substrate specificity for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.5. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan. The designed protein sequence is ",
    "response#1": "MAEPSTLLNSRQINQGDVFCAVIGTASDGRNYIDQAIAAGASLVIAECQQPQQHGNQLVREKTTGKVTKKVTIVQFYQLNQHLFELAASYYGRPSERLQLVGVTGTNGKTTISQLLAQWANLLGHQAGVMGTTGNGLLGQIVPAANTTGSAIEIPHVLAEMVQQGADYAAMEVSSHGLVQGRISALQFQASIFTNLSRDHLDYHGDMASYAGAKKTLFTAHHCGKAIINADDAVGREWLLTLPEAVAVAQEKISLSGWAGQALWLEQVHYSTQGVTVRFDSSWGEGEFCAPLVGAFNVMNLMLALATLLALDVPLDACIGAISRLSPVPGRMEQVALPGQPLVAVDYAHTPDALDQALQALAPVATARGGRLWCVFGCGGNRDAAKRPLMGGVAQQRADQVIVTSDNPRGEDPAEIIRQILQGTIAGLTVRAEPDRATAIATALQEAGADDVVLIAGKGHEDYQEIAGKRLPFSDQAVARAALAERGVAA",
    "response#2": "MRLHELADILGVGLQTDSRRVSPGDGFIAWPGAATDGRAHVGDALARGAAACLVEQEGTDDVPTVTVADSRRAFGLLADRLYGGYPERMIGVTGTNGKTSTSQMIASLLNLCQRPAATLGITGTNGKTTTSHLIEKICRDAGMRTGLIGTMYTKVGETIHETKNTTPDSVTLQKTFAEMNREHVDTAVMEVSSHALELGRVHGCDYDIAVFTNLTQDHLDFHETMDKYKHAKSLFFSQLGNSYIEKRPKFAVLNADDEATKDFIKYTAAHVVTYGIDNEADIMARDIKITASGTEFLLVSPVETLPVKLQLVGKFSVYNALAAIGAAVSLGVDVTSIADALAAVPTVPGRLEEVPAPGFRVFVDFAHTASALARMLDSLRGAARETGGRLIVVFGCGGDRDRTKRPEMGRAVCARADAVYLTSDNPRTEEPRAIIDDVLAGTAGGDADLHVEADRERAIRSALADAREGDVVVIAGKGHETYQVVGTEKRPFDDREVAREAIARRLGS",
    "response#3": "MNLKKLLEGLNYECIEGNTNIEVKGIAYNSKKVKPGDLFVALKGRNTDGHLFIEEALKNGAVALMVQEERNYPVPYIYVENTRKYLGIISAKFYGNPSEKLNIIGITGTNGKTTTATLLYRMFRQLGYKCGLISTIANFIDQEEVPATHTTPDAMTINGLLDKMFQKDCSHCFMEVSSHSVVQNRITGLHFAGGIFTNLTHDHLDYHQTFAAYRDAKKRFFDTLPSRSFALINDDDKNGQIMVQNCGARKFTYACKKMADFNARVLEHSVEGMQLGIDGKEIWSSFIGMHNAYNLLAVYACAIKCGLNIEDIKKGIENVNVPGRLERVGEVDGALCIVDYAHKPDALAHVLDALRPFTKGRLVCVVGCGGDRDRGKRPLMGRIAVEKADTVIVTDDNPRSEDPAAIRAEVMKGAPGATEIGDRAEAIRAAVRGLRPGDVLVVAGKGHETYQIFKDKTIDFDERQIVIETLNKK"
  },
  {
    "reference": "MPGLETYDAIMLLSYGGPNGPDDVLPFMRNATRGRGIPDERLLQVAAHYKRFGGVSPINACNQRLIADLSAELARRGHDIPVGWGNRNWHPFVAEGLDALAGAGARRILVLPTSAYASYSGCRQYREDLAEAAAALREKWGTIVLGAEDSAGNPDADIILDKVRPYYSTPGMASAQVASVRRAWEALVARGVDADGIRLIFVTHSIPVSMEAGSSPFPFRPSIDEAVAASGGRAEQQGSEASSHAGTPATEISYVAQHHALIQAIMPELRRVLGRADLGYDLVYCSRSGPPQARWLEPDINDFLEEIAADATPLSGVVVVPIGFICDHMEVVYDLDTEAKETAAHLGIPYERADTVSTDPGFVSSLVDVLEERAAQARGERPARATVTGVGPFHTVCPSDCCLSPARPGHPSPAAGGAHPGAASTPHTSGAPARAAGQPAPSQEDSMSTPHPHAVVPPEQNPANPGHPAGVPDRVGEHAARHQARHAGTEATPHSHAAHARVTDPRDATDVDFDEVNNKQHYALYSVFALGESLPADDGERGRIVAESLDYVKGAGAEIRGFYDVSGFRAEADLMVWWLDDDPEVLQDAYHRLRASALGKFLDPVWSCMGLHTPAEFNKRHIPACFGGVAPRDWAMVYPFVRSYDWYLKAPEERARIMAEHGRNGFAQYPDVKGSTLSAFGFSDYEWVLAFEADTLDRLEGVMHAQRYTEARLYVREDTPFFTGPRVSLGEWAERQPRA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that is capable of catalyzing reactions within the Porphyrin-containing compound metabolism pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "VGGGPGDPELLTLKALRLIQQADLIVYDRLVSPEVLNLARRDAERIYAGKKRSEHTLSQGSINNLLIKHAQLGKRVVRLKGGDPFIFGRGGEEIETLLEKKIPFEVVPGITAASGCASYAGIPLTHRDYAQSCVFVTGNLKENKVNLDWDQLAKPNQTIVIYMGLVGIHEVCRELIAHGLPKNTPAALIQKGTTVEHKVFISNLNELPSTVSKEKPKPPTLIIIGHVL",
    "response#2": "VGGGPGDPELLTLKALRLIQQADLIVYDRLVSPEVLNLARRDAERIYAGKKRSEHTLSQGSINNLLIKHAQLGKRVVRLKGGDPFIFGRGGEEIETLLEKKIPFEVVPGITAASGCASYAGIPLTHRDYAQSCVFVTGNLKENKVNLDWDQLAKPNQTIVIYMGLVGIHEVCRELIAHGLPKNTPAALIQKGTTVEHKVFISNLNELPSTVSKEKPKPPTLIIIGHVL",
    "response#3": "VGGGPGDPELLTLKALRLIQQADLIVYDRLVSPEVLNLARRDAERIYAGKKRSEHTLSQGSINNLLIKHAQLGKRVVRLKGGDPFIFGRGGEEIETLLEKKIPFEVVPGITAASGCASYAGIPLTHRDYAQSCVFVTGNLKENKVNLDWDQLAKPNQTIVIYMGLVGIHEVCRELIAHGLPKNTPAALIQKGTTVEHKVFISNLNELPSTVSKEKPKPPTLIIIGHVRT"
  },
  {
    "reference": "MKRTYQPNRRKRAKTHGFRKRMSSVGGRRIIAARRRRGRKRLAV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The ideal protein would be one that plays a critical role in translation through structural constituent of ribosome within ribonucleoprotein complex, ribosome. The designed protein sequence is ",
    "response#1": "MPFAPRP",
    "response#2": "MKAIVFAYHDMGCTGIRSFLEAGYEIAAIFTHADNPGAPSFTEIAAL",
    "response#3": "MPVKFYLVEGRMLIRHDRMPEWWKFRKYVRALKPEHAVEKVLSELGSNHKVKRYHVKIERS"
  },
  {
    "reference": "MSPRGKKQEKPQPPPANKGFRFGSPLGYIALLLLGFMLFKNVFQDAGVQRKTYSEFRTAIQEDKFSKVQIAPDWVKGFLKDNSAARASGTPAGGTERLRPELSDLPWMAYRVEGDEQLVPLLEQKGVPYEAVVQSGFSDVLLIWLLPLAFAFVLWSFMMRRMAGGMGQGPQSVMSFGKTRAKVQAEADTGVGFKDVAGVDEAVDELREIVEFLKTPEKFRRLGGRIPKGVLLVGPPGTGKTLLARAVAGEAGVPFFSLSGSEFVEMFVGVGAARVRDLFAQATAKAPCIIFIDELDAIGKSRNAGVAGGHDEREQTLNQLLAEMDGFDSRAGLIILAATNRPEILDSALMRPGRFDRQVLVDRPDKRGRERVLEIHSRGVKLGPDVDLKSIASRTPGFAGADLANVVNEAALLAARRNRDFVMRADFEEAIERVVAGLEKKNRRMNEREKDIVAHHEAGHAVVGWMLPNAERVTKVSIIPRGIAALGYTMSLPLEDRYLMSYDELRDKMASMMGGRAAEEIFIGEVSTGASNDLKQATEIAKLMVRDYGMSSLGPVALGSEQNAFLRSAGGPEMRSYSEQTARMVDDEIRKMVTEALDRAREVLTTHRDKVEALAARLLATEVVDEDEILRILGPKATAARGLLHPEARQVISAHPASSDETPPPGAQHSSGNLPDA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP, Zn(2+) binding.2. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity.3. The designed protein should be involved in cell division, proteolysis, protein catabolic process by localizing to plasma membrane and exhibiting zinc ion binding, ATP binding, ATP-dependent peptidase activity, metalloendopeptidase activity, ATP hydrolysis activity.4. For general function, the protein need meet that Plays a role in the quality control of integral membrane proteins.; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins5. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.6. The protein design should emphasize Polar residues compositional bias.7. The designed protein should contain one or more AAA+ ATPase domains that are essential for its function.8. The designed protein must have a active site active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "MQFILALVISLSSLSDLGKSPSGGSSGGGLFSGGQTVSIPYSEFKAEVKAGNVRTVTFGETSATGTFLTPKNQPDGQIPASGFRTQLPPYDDPELSKILSDQGVVVTAGETATTESDGADSSWWTQLLMIALPFILLLAIFMFLFNQAQGGGNKVMNFGKSKAKLASKNTPKITFKDVAGEDEAVEELHEIKDFLENSSKYQALGARIPRGVLLVGPPGTGKTLMARAVAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRNSPCIVFVDEIDAVGRQRGAGLGGSHDEREQTLNQILVEMDGFDTNTGVIVVAATNRPDVLDPALLRPGRFDRQVILDRPDLEGRKAILKVHAKGKPIAEDVDLEVIARSTPGFSGADLANIVNEAALIAARKGHGKIMMEDFEEAVDRVVAGPEKKSRRVISDKEKRVTAYHEAGHALLALLLPNCDPLHKVTIIPRGMAGGITWTLPQEDKHFYYKSELIDTITEMFGGRAAEELIFSEISSGASNDIKVASKMVRKMICEWGMSERIGPIAMGNNEEYVFLGKDLGHDVNYSQATALDIDREVRRIIDFCHNQSRNILSEKRELLIVLAEALLEKELLSGEELVEIFGPVPWEERPSTSETPTPKPTPPTPPAPDSPTPETA",
    "response#2": "LLVGPPGTGKTLLAKAVAGEADVPFFSMSGSDFVEMFVGVGASRVRDLFRQAKEKAPCIIFIDEIDAVGRARGRNANFGGNDERENTLNQLLTEMDGFGTNSGVIILAATNRVDILDKALLRAGRFDRQIHVLYCTATMKEDPRVKVISEKEKRLTAVHESGHALVAYLLPEADPVHKVTIIPRGRAGGYTMMLPDEDRSYETKSYYLAQIRVALGGRAAEQIVFNEISSGASGDLQNVTHIVRQMITRLGMSPKLGPMVFGEQQDQVFLGKSLGHERNYGEGVAELIDKEMHDLVTTAYDDVIRMLEEHRDALNHMAAALMEVETINHKQVENLIGLP",
    "response#3": "LLVGPPGTGKTLLAKAVAGEAEVPFFSLSGSDFMEMFVGVGASRVRDLFKQAKEKAPCIIFIDEIDAIGRSRAKNASFSSSEERENTLNQLLTEMDGFATNTGIIVLAATNRADILDGALMRAGRFDRQIHVLSLSDPTIEGRLQIIARHEGVKAEIIARGTPGMAGADLANLVNEAALFAARAGKRLVDMDDFTDEQSLVDALPTHYENRMDISELMNQIIVAVTVVDSFIGNKTDKSEITYTNFLTQVQQKKVDSVQITADHSITGQLKDGSSFTTYAPTDAALMPALKDSDVNVVAKPVDNSRRQGTRQKAHRVAVEFSKIILPDN"
  },
  {
    "reference": "MALTKAELTDLLFENIGLNKREAKEIVECFYEEMRAALQNGDGVKLSGFGNFQLRTKPQRPGRNPKTGEEIPISARRVVTFHASQKLKSMVEANYRGESGTN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein with structural constituent of chromatin is needed to enhance DNA recombination, regulation of DNA-templated transcription in a specific context. The designed protein sequence is ",
    "response#1": "QGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKR",
    "response#2": "QGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKR",
    "response#3": "QGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKR"
  },
  {
    "reference": "MMKMETDKIMDETNSNAQAFTTTMLYDPVRKKDSSPTYQTERELCFQYFTQWSESGQVDFVEHLLSRMCHYQHGQINAYLKPMLQRDFITLLPIKGLDHIAENILSYLDAESLKSSELVCKEWLRVISEGMLWKKLIERKVRTDSLWRGLAERRNWMQYLFKPRPGQTQRPHSFHRELFPKIMNDIDSIENNWRTGRHMLRRINCRSENSKGVYCLQYDDGKIVSGLRDNTIKIWDRTDLQCVKTLMGHTGSVLCLQYDDKVIISGSSDSTVRVWDVNTGEMVNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMTSPSEITLRRVLVGHRAAVNVVDFDEKYIVSASGDRTIKVWSTSSCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNSIRLWDIECGACLRVLEGHEELVRCIRFDTKRIVSGAYDGKIKVWDLVAALDPRAASNTLCLNTLVEHTGRVFRLQFDEFQIVSSSHDDTILIWDFLNFTPNENKTGRTPSPALMEHXHFVQRQLRELHLQSSSEGEEDDDADEDDEDDFEEEDSRNDAGAFMGGYVRHRDAGGSGSVTGSGSDPESDDLDFDIDVENINDYDE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The presence of F-box domains is critical for the interaction of the designed protein with its intended targets.2. Prioritize Acidic residues compositional bias within the protein sequence to achieve desired characteristics.3. The designed protein should have molecular adaptor activity, ubiquitin ligase-substrate adaptor activity, phosphoprotein binding within nucleus to regulate positive regulation of proteasomal ubiquitin-dependent protein catabolic process, establishment of epithelial cell polarity, positive regulation of Toll signaling pathway. The designed protein sequence is ",
    "response#1": "LVEKYQQSWKTLFLKKPRSRFDGCYISVCRYFRPGTSDTSWNQPIHLITYYRYLRLYPNSTCIVYQSSNEPNDVVRNFSVQNTSLFSPMSSSPMFSNGNVALTGSWSMTPSGEMLIVYPASQTYTYVQKLQVRC",
    "response#2": "VSWSIIQTRPHKLTLEIVGHHGCKTWGLTRSPGSLVSFFAETTIRKLQVLACTDGLVLLYVESCVGTPMYYVGNPLFQEWFRIPLRPKYTSQNVEKLRNHERFSDSGLVTKMQSGIVVSYKVVWLIAHTFAQVDFAIYSSNTGEWEIKNVTCLHSAYWFSHHKSIALNGILHWLSNLTGSFLAYDFYGGHHDACSIIYFPDNGKDYELPRFRRTITTSEGSIVYFNEFGGNANRKVRVWRLVKYTDGPEAWQLFWEASLASVTKLGIDYFPVVMHPLKSEIIYLWSRNKKGMVLFNLRTHVFSLHKESEDERKCMDGCTLSFNWCNEYMETVHRYFSPSFQGGPNLLLASQYVLPRWLSRLPN",
    "response#3": "TGKLIRRLSGHSDGVKTLYFDDRKLITGSLDKTIRVWD"
  },
  {
    "reference": "MKSLQGKTILLGITGGIAAFKAASLTSMLVKKGAEVHVIMTESAKQFITELTLQSLSKQRVYSDTFQERDPSSISHIDLADSADLVLVAPATANILAKMAHGLADDMLSTTLLAATSPVMVAPAMNVHMYQHPAVLNNIDILSRRGIQFIEPGEGLLACGYVGKGRLEEPEAIVRCVESFFALSSRPESQPEQPLKGRKVVITAGGTVERIDPVRYISNDSSGKMGFALARAALAMGAEVTLVAARTDEEPPQAAGLRVVKVESAQQMYDAVLGVWEDCDILIKAAAVSDYRPKEQAAAKIKKSGDTMLLELVKTTDILETLGKTKNKQFLVGFAAETGDTEAFAKEKLVRKNCDLIVANNVAEAGAGFGLDTNIVSIYDTQGVVAQWPLLTKDEVARRLLLLFAERMTGISV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should contain one or more DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains that are essential for its function.2. The protein design must be able to perform phosphopantothenoylcysteine decarboxylase activity, metal ion binding, FMN binding in order to contribute to coenzyme A biosynthetic process.3. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A.4. The CTP binding site should be located in a region of the protein that is accessible to the ligand.5. The protein design should consider the endogenous proteins and enzymes present in the CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis, Cofactor biosynthesis pathway to avoid interference with native metabolic processes.6. Mg(2+) is required for the proper folding and stability of the protein.7. The Proton donor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "MKIALGVTGGIAAYKAAEVLRLLQDRGVTVQVIMTRAAQEFVRPLTFAALSGQKVITEMFGEGNAEPNVDSAVEHISVAQGIDALLVVPATADSLAKFAQGIANDFLSTLYLATAAPVIVAPAMNVNMWEHPATKINLETLRARGVHVIEPGAGYLACGMVGAGRLAEPEHIATETDRIAAEIAALVGPLRGRKVLVTAGPTYEDLDPVRYVGNRSSGRMGFALAAEAARRGAVVTLIAGPTSLDPPSVHQVVRVRRAAEMHAAVLEQAGDAAVVIMAAAVADYAPEQRVNQKVHKGADTITLVLTRTPDILADLGARRLAAGTGPVLVGFAAETEDVVARAAEKLHRKHADLIVANDVSRGDAGFEVATNAVTLLFPGVGAPEELPLMSKRDVARAIVKRAARLLGR",
    "response#2": "MKIALGVTGGIAAYKAAEVLRLLQDRGVTVQVIMTRAAQEFVRPLTFAALSGQKVITEMFGEGNAEPNVDSAVEHISVAQGIDALLVVPATADSLAKFAQGIANDFLSTLYLATAAPVIVAPAMNVNMWEHPATKINLETLRARGVHVIEPGAGYLACGMVGAGRLAEPEHIATETEALARATRSVLEQGDALLEGHRVVITAGPTREAIDPVRYITNHSSGKMGFALADAFRLAGADVCVIAGPVSLSLHPQIQRIDVESAHQMYDAAMEQAALGFDIFVGSAAVADYAPVQQSSSKLKKNDQNLVIELQENPDIIASVAQGDNASRLIVGFAAETNDVEAYAQGKLERKALDLVIANDVSNQSIGFNSDHNCVTLITPQEVETWPTMDKVQVAWRLAHRIALHWAG",
    "response#3": "MKIALGVTGGIAAYKAAEVLRLLQDRGVTVQVIMTRAAQEFVRPLTFAALSGQKVITEMFGEGNAEPNVDSAVEHISVAQGIDALLVVPATADSLAKFAQGIANDFLSTLYLATAAPVIVAPAMNVNMWEHPATKINLETLRARGVHVIEPGAGYLACGMVGAGRLAEPEHIATETGRVIEAVGEKDASDSQRRIAEINTGILAAPSARLKQWLDQLDNNNAQGEYYLTDIVAMAVAEGVPTVSAQPTEAVEVEGANNRVQLARLERAFQARQAEQLMADA"
  },
  {
    "reference": "MASLSSSFLVLFLLFQNSCYCFRSPLSVFKRYEESTRSLSNDCLGTTRPVMSPGSSDYTLDIRMPGVTPTESDTYLCKSYRLPVDDEAYVVDFRPHANMDTAHHMLLFGCNIPSSTDDYWDCSAGTCMDKSSIMYAWAKNAPPTKLPEGVGFRVGGKSGSRYFVLQVHYGNVKAFQDKHKDCTGVTVRVTPEKQPQIAGIYLSMSVDTVIPPGEEAVNSDIACLYNRPTIHPFAYRVHTHQLGQVVSGFRVRHGKWSLIGRQSPQLPQAFYPVEHPVEISPGDIIATRCLFTGKGRTSATYIGGTSNDEMCNLYIMYYMDAAHATSYMTCVQTGEPKLFQNIPEIANVPIPVSPDMMMMMGHGHHHTEAEPEKNTGLQQPKREEEEVLDQDVHLEEDTDWPGVNLKVGQVSGLALDPKNNLAIFHRGDHVWDENSFDRNFVYQQRGIGPIQESTILVVDPSSSKVLKSTGKNLFFLPHGLTIDRDGNYWVTDVALHQVFKLGAGKETPLLVLGRAFQPGSDRKHFCQPTDVAVDPITGNFFVADGYCNSRIMQFSPNGMFIMQWGEETSSNVPRPGQFRIPHSLTMVPDQGQLCVADRENGRIQCFHAETGNFVKQIKHQEFGREVFAVSYAPGGVLYAVNGKPYYGYSAPVQGFMLNFSNGDILDTFIPARKNFDMPHDIAAADDGTVYVGDAHANAVWKFSPSKAEHRSVKKAGIEVEEITETEIFETHIRSRPKTNESVEKQTQEKQQKQKNSAGVSTQEKQNVV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that contributes to peptide metabolic process by functioning in transport vesicle membrane with peptidylamidoglycolate lyase activity.2. The Cu cation binding site should be located in a region of the protein that is accessible to the ligand.3. The protein should have Helical domains to ensure proper insertion into the membrane.4. For general function, the protein need meet that C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.5. Include a signal peptide as an essential component of the protein.6. The Intragranular topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "FKDAGKPVIAYADNYSQGQYYLASFADEIYLNSIGSVDIHGLSQENLYFKEMLDKLAVTPHIFRVGTYKSAVEPFLRNDMSAEAKANMQRWLGEMWNNYVLSVSENRNIKKDRILPNAKQYLAELKALKGNSTAYAQQRGLVTDVVTRLDLDKKA",
    "response#2": "YAANITKSVFDYFEDYFGMSYSLPKLDKIAIPDFGTGAMENWGLITYRETNLLYDPDESASSNKQRVAAVIAHELVHQWFGNIVTMEWWDDLWLNEGFASFFEYLGVAYAEKDWQMRDQMILDDVLPVQEDDSLMSSHPIVVTVATPDEITSVFDGISYSKGASILRMLENWITREKFQIGCQNYLKKHKFENAKTSDFWAALEEASNLPVKEVMDTLFG",
    "response#3": "FKDAGKPVIAYADNYSQGQYYLASFADEIYLNSIGSVDIHGLSQENLYFKEMLDKLAVTPHIFRVGTYKSAVEPFLRNDMSAEAKANMQRWLGEMWNNYVLSVSENRNIKKDRILPNAKQYLAELKALKGNSTAYAQQRGLVTDVVTRLDLDKKA"
  },
  {
    "reference": "MNDFVFLVVACLLTAGTEGKKDGYPVEGDNCAFVCFGYDNAYCDKLCKDKKADSGYCYWVHILCYCYGLPDKEPTKTNGRCKPGKK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Incorporate a signal peptide in the protein design.2. A protein that can perform sodium channel inhibitor activity, toxin activity in extracellular region to support defense response.3. For general function, the protein need meet that Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission.4. The designed protein have LCN-type CS-alpha/beta domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "KEGYPVGRDGCKISCVINNNFCKVECQAKWRQSDGYCYFWGLSCYCTNLPEDAQVWDSSTNKCS",
    "response#2": "KDGYLVNKSTGCKYSCIENINDSHCNEECISSIRKGSYGYCYKFYCYCIGMPDSTQVYPIPGKTCG",
    "response#3": "KEGYPVGRDGCKISCVINNNFCKVECQAKWRQSDGYCYFWGLSCYCTNLPEDAQVWDSSTNKCN"
  },
  {
    "reference": "MAKVLVLYYSAYGHIETMANAVAEGAREAGATVDIKRVPELVPPEIAKASHYKLDQAAPIATVDDLANYDAIIIGTGTRFGRITSQMANFLDQAGGLWAKGVLHGKVGGAFTSTASQHGGQETTLFSIITNLLHFGMVVVGLNYGYGGLTTLDEVAGGSPYGATTITGGDGARQPSANELGGARYQGKVIAETAIKLHG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be able to bind FMN, NAD(+), substrate ligand in a variety of conditions2. The designed protein must be able to perform NADPH dehydrogenase (quinone) activity, flavin adenine dinucleotide binding, NAD binding, FMN binding under specific conditions. The designed protein sequence is ",
    "response#1": "MKTLVILSSILGDRSHSKQLADHLIQRLRQHEPDGSIRVRDLAADPVPYFDGATAGALFTPADQRTAEQAAIVARSDALVAELFDADRIVFAVPVYNFGLPAQLKSYIDQIARAGVTFRYTAQGVPEGLLKNKQVVVLSARGGKAEGTPADTLTPYLAQVLGFVGLVDPVFISAEGMAMGELAAQDGLALARQRIDEL",
    "response#2": "MKTLVILSSILGDRSHSKQLADHLIQRLRQHEPDGSIRVRDLAADPVPYFDGATAGALFTPADQRTAEQAAIVARSDALVAELFDADRIVFAVPVYNFGLPAQLKSYIDQIARAGVTFRYTAQGVPEGLLKNKQVVVLSARGGKAEGTPADTLTPYLAQVLGFVGLVDPVFISAEGMAMGELAAQDGLALARQRIDAV",
    "response#3": "MKTLVILSSILGDRSHSKQLADHLIQRLRQHEPDGSIRVRDLAADPVPYFDGATAGALFTPADQRTAEQAAIVARSDALVAELFDADRIVFAVPVYNFGLPAQLKSYIDQIARAGVTFRYTAQGVPEGLLKNKQVVVLSARGGKAEGTPADTLTPYLAQVLGFVGLVDPVFISAEGMAMGELAAQDGLALARQRIDG"
  },
  {
    "reference": "MGYKVAVVGATGNVGREMLTILSEREFPADEVTALASSRSVGMEVSFGDNTLKVRDLETFDFAGADIVLSSPGAAVSRIHAPRAAKAGAVVIDNTSCFRMEMDIPLVVPEVNPEAIGGYTKRNIIANPNCSTIQMVVALKPLHDLAPIKRVVVSTYQSTSGGGKEAMDELFNQTRGIFVNEPVLKHQKIFTKQIAFNCIPHIDVFMEDGATKEEWKMAVETRKIMGADIQVFATCVRVPVFIGHAEAVNVEFTRPVSEKEARAALKKAPGVTVVDHRADEGYVTPAECSGEDAVYVSRIRKDPTVKNGLAFWCVSDNLRKGAALNAVQIAEELVKSFL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have a binding site that is specific to the target NADP(+), phosphate ligand.2. The presence of Semialdehyde dehydrogenase NAD-binding domains is critical for the interaction of the designed protein with its intended targets.3. The Proton acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.4. The designed protein should be optimized for NADP binding, aspartate-semialdehyde dehydrogenase activity, NAD binding activity in order to improve threonine biosynthetic process, isoleucine biosynthetic process, lysine biosynthetic process via diaminopimelate.5. A protein that can modulate the overall flux of metabolites through the Amino-acid biosynthesis, L-threonine biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "LGGLFEKDLVEWVSVATYQAASGAGAKNMRELLVQMGELRDVVKTDLANPASSILEIERAVADKMRGDLPIENFGAPLAGSLIPWIDKLLDSGQTKEEWKGYAETNKILGLSDSPIPIDGLCVRIGALRCHSQAFTIKMKKDLPLAEIEQILAAHNEWVKVIPNDKEATLRELTPTKVTGTLSVPVGRLRKLNMGPEYLAAFTVGDQLLE",
    "response#2": "LGGLFEKDLVEWVSVATYQAASGAGAKNMRELLVQMGELRDVVKTDLANPASSILEIERAVADKMRGDLPIENFGAPLAGSLIPWIDKLLDSGQTKEEWKGYAETNKILGLSDSPIPIDGLCVRIGALRCHSQAFTIKMKKDLPLAEIEQILAAHNEWVKVIPNDKEATLRELTPTKVTGTLSVPVGRLRKLNMGPEYLAAFTVGDQLLK",
    "response#3": "LGGLFEKDLVEWVSVATYQAASGAGAKNMRELLVQMGELRDVVKTDLANPASSILEIERAVADKMRGDLPIENFGAPLAGSLIPWIDKLLDSGQTKEEWKGYAETNKILGLSDSPIPIDGLCVRIGALRCHSQAFTIKMKKDLPLAEIEQILAAHNEWVKVIPNDKEATLRELTPTKVTGTLSVPVGRLRKLNMGPEYLAAFTVGDQLLHVKPPSMVLKPIHDVSRIKRIVVATYQAVSGTGKRAIEELSNQTQAIFNLKEIVCNVYPHQIAFNCLPHIDIFLENGYTKEEMKMCYETKKIMEDDSIEVTATCVRVPVFYSHSEAVNIELEKRLTANEVRDILSNAPGVKVADNPDKNEYPLAIYAAGRDEVFVGRIREDASKENCVDMWIVSDNIRLKPLHDIVPIRRVVVSTYQSVSGAGMGAMEELEAQVRGELFNEQAAIERLPRRIAFNVVPHIDVFEDDGSTREEWKMVHETRKILDPEIALAATCVRVPVFIGHAEAVNVEFAGPVSPSDATDALMVAPGVEVVDADDVQMYATPIDCVHEDPVFVSRIRTDPTVDHGLQFWTVADNLVLSLKPIHDVSRIKRIVVATYQAVSGTGKRAIEELSNQTQAIFNLKEIVCNVYPHQIAFNCLPHIDIFLENGYTKEEMKMCYETKKIMEDDSIEVTATCVRVPVFYSHSEAVNIELEKRLTANEVRDILSNAPGVKVADNPDKNEYPLAIYAAGRDEVFVGRIREDASKENCVDMWIVSDNIR"
  },
  {
    "reference": "MHPLLPTTTALPLFDVAASRRIEARALLHSPDLMERAGLAVAKLALALRRGSGPVWVVCGPGNNGGDGHVAARRLVQAGVPVHVGHDAPERPALVIDALLGLGLNRAPSRELLGVIAAMQACGAPILAVDLPSGLRADTGQPAGDQAVRASHTLTLLTLKPGLVTGEGRAHAGRIWFDDLGVAPDEAPTAELLGPAPPLPRSPTSHKGSHGQVLIVGGAPGMQGAARLAARAALACGAGRVYLDLLGLPADEVADPGRPELMRGRLANVPQAVGVIGCGAGTGIAAALPSLLRTLPRLVLDADALNAIAGDSALRDLLLARSPGSSLLTPHPLEAARLLGTDVTSVQADRLAAARDLAARLNCTVVLKGSGSVIASQGERPAICASGGPALATAGSGDVLAGWLGGLWAQQPAAVAHAVACAGVEAHGRAGEVDGVLRAGALIERLAARR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The AMP, K(+) binding site should be stable and able to withstand changes in environmental conditions.2. The designed protein should be optimized for NADPHX epimerase activity, ATP binding, metal ion binding activity in order to improve nicotinamide nucleotide metabolic process.3. The protein requires Mg(2+) to activate a critical catalytic site.4. We need a protein that can perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate with high efficiency.5. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..6. The protein contains novel YjeF N-terminal domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MRLLEKRALESGTSFGDLMHNAGRAVASEICGLFDSFPGKKALVLVGPGNNGGDGLVAARFLNDAGAITHVYLLAPRDPGDLVYNEALEAGISPIEVESDAGFSKLRELLGEADVVVDAIFGTGLGRPIGGASAAALTLVAEARNQRPDMVVIALDLPSGLNADSGEIDDSTLAVDYTITLGYAKRGFFLFPGADYTGQILVADIGIPDGLDDRIDMCRALPQRPGNAHKNQCGRVLVVAGSTGYTGAAALCARAAQKSGAGLVSLCIPRSLDNILEIKLTEEITCPLADGNKGYLTEKNADAILALAGEYQVLAIGPGLGRNASTAAMVRKVVARSPVPVVLDADGINAYEKAGHLLKKAKSPVIITPHDGELKRLTGEKEIGWGPARIRALRNIQKELGITIVLKGGPTLVVSSTCMVNVNTTGNPGMATAGAGDVLTGLISGLYAQTHNLEQAVLTGVYLHGLAGDYGAMACTEYCLTAGDIITFFPQAFMEGPGDVRLVLRRREKSSHKGDNGRVLIVGGGPFAGAPALSALASLRTGADWVTVAAPRNVASIIASISPNLIVQPLSGSGSGSILTEDDVSLVSSLMQMHDVLVIGMGLGAAEETKRAVKLIIEDEAVKKVVVDADGLYGLDLPLKVSKLKSKLKSKQVIITPHAGEFSKMDAQRPPPDGEERLGFIQEFSLRNRVVTLLKAAHDVISDGKRIKVNATGNAGMTVGGTGDVLSGITGALFATSEDAFEVATAAAFISGSAGDAAFRDKGLSLLATDVVEAIPGILRA",
    "response#2": "MATLPESALMQRAAAGLAAVCARVLGGPYGSRVVLLVGGGDNGGDALWAGARLAHRGASVDAVLLRHDRVHPAGLAAFRRAGGRVVSSADVPLTPETQRAIARADLVLDGMLGIGGRGGLRDDAARLADAAAAEAGLVVAVDLPSGVEPDTAELTGPHVRADVTVTFGTHKLAILADPAAQVAGHVEFVDIGLAPYLPREPDLVAGGSGDVLAGVPEPVDVAEMLPAPERNSDKYRRGVLGLLAGSAQFRGAAVLAAGGAAEGGAGMVRFVGPDEVATQVLNRWPEVVTSADVADTGRVQAWAVGSGLGDGRAAEVAAVLDTGLPVLVDADGLRHLPETLDGRALLTPHAGELARMMDADRGDVEAHRLRYVQAAARRWNATVLLKGSTTLVAAPDGRIRVNPTGTPALATAGSGDVLSGLAGALLATGMEPFDAGSAAAYIHGLAGQAAAEGSGLPSASAVLDALPAVLAALHG",
    "response#3": "MAALDANCEFFGLTPLQLMENAGASIASELKKRFAEDTEVVIIAGKGNNGGDAFVAARHLRNANVRVILVGRSKDLRTEASSRNWRILSECGYLLDEITDSSTLKMLKETLNSEVIIDALLGTGVRGKIREPESTAIELINESGAFVLAVDVPSGLNPDTGEYDKAVRADLTVTFHRAKPGLLKKESKSYVGELVVADIGISQRAIDAVGPRVENSHKGTFGTLAILAGSADMPGAAYLCATGALHAGVGLVKLASDASALGVLKNRLSEPVFLSGGAEAAAQTSKTALLVGCGLGRGFDASLGALLRGSDVPTVIDADGINFLASHIDVLKEMQAPAALTPHPMELSRLTGCGVEEINRNRVRTARAFAAEHGCFLLLKGSRTVIAAPDGRVRITVSGCSALAKGGSGDVLAGVVGALAAQGYDLFDALTVGAFLHGRAGERLAARFGAHGALPSQLPEELCR"
  },
  {
    "reference": "MPDLADLFPGFGSEWINTSSGRIFARVGGDGPPLLLLHGFPQTHVMWHRVAPKLAERFKVIVADLPGYGWSDMPESDEQHTPYTKRAMAKQLIEAMEQLGHVHFALAGHDRGARVSYRLALDSPGRLSKLAVLDILPTYEYWQRMNRAYALKIYHWSFLAQPAPLPENLLGGDPDFYVKAKLASWTRAGDLSAFDPRAVEHYRIAFADPMRRHVMCEDYRAGAYADFEHDKIDVEAGNKIPVPMLALWGASGIAQSAATPLDVWRKWASDVQGAPIESGHFLPEEAPDQTAEALVRFFSAAP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a high affinity for substrate.2. For general function, the protein need meet that Has lower activity towards bromoacetate and chloroacetate.; Catalyzes the hydrolytic defluorination of fluoroacetate to produce glycolate3. The protein must be able to haloacetate dehalogenase activity.4. The designed protein must have at least one AB hydrolase-1 domain.5. The protein's Nucleophile, Proton acceptor must be carefully engineered to ensure that the amino acids involved in a haloacetate + H2O = a halide anion + glycolate + H(+) are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "RTNTTILEGYAAEPSRQTLSKISNAFKERGTKFDFRVMATHGGTISWKAKELARTIVSGPIGGVIGAKYLGEVLGYKNIACSDIGGTSFDVALITQGEMTIKNDPDMARLVLSLPLVAMDSVGAGAGSFIRLDPYTRAIKLGPDSAGYRVGVCWKESGIETVTISDCHMVLGYLNPDNFLGGAVKLDRQRSVDAIKAQIADPLGLSVEDAAAGVIELLDSDLRDYLRSMISGKGYSPASFVCFSYGGAGPVHTYGYTEGLGFEDVIVPAWAAGFSAFGCAAADFEYA",
    "response#2": "RTNTTILEGYAAEPSRQTLSKISNAFKERGTKFDFRVMATHGGTISWKAKELARTIVSGPIGGVIGAKYLGEVLGYKNIACSDIGGTSFDVALITQGEMTIKNDPDMARLVLSLPLVAMDSVGAGAGSFIRLDPYTRAIKLGPDSAGYRVGVCWKESGIETVTISDCHMVLGYLNPDNFLGGAVKLDRQRSVDAIKAQIADPLGLSVEDAAAGVIELLDSDLRDYLRSMISGKGYSPASFVCFSYGGAGPVHTYGYTEGLGFEDVIVPAWAAGFSAFGCAAADFEYTPDTDKEKAAATLQAAWEELTKNVLEEFKLNGYSADQVTLQPGYRMQYRGQLNDLEIESPLAQAHTAADWDQLTDAFNATYGRVYAASARSPELGYSVTGAIMRGMVPIPKPKIPKEPEEGETPPESAKIGTRKFYRKKRWVDAQLYHMESLRPGNRVMGPAVIESDATTFVVPDGFETWLDGHRLFHLRER",
    "response#3": "RTNTTILEGYAAEPSRQTLSKISNAFKERGTKFDFRVMATHGGTISWKAKELARTIVSGPIGGVIGAKYLGEVLGYKNIACSDIGGTSFDVALITQGEMTIKNDPDMARLVLSLPLVAMDSVGAGAGSFIRLDPYTRAIKLGPDSAGYRVGVCWKESGIETVTISDCHMVLGYLNPDNFLGGAVKLDRQRSVDAIKAQIADPLGLSVEDAAAGVIELLDSDLRDYLRSMISGKGYSPASFVCFSYGGAGPVHTYGYTEGLGFEDVIVPAWAAGFSAFGCAAADFEYPE"
  },
  {
    "reference": "MIFSTLEHILTHISFSIISIVITIFLISLSVDEIVGLYDSSEKGMTATFFCITGLLVTRWIYSGHFPLSDLYESLIFLSWSFSVIHMVPYFKKHKNYLSTITAPSTIFTQGFATSGLLTEMHQSEIVVPALQSQWLMMHVSMMILGYAALLCGSLLSVALLVITLRKAIRVFSKKNNFLNESFSFVEIQYMNERSNVLLTTSFCSSRNYYRAQLIQQLDQWSYRIISLGFLFLTIGILSGAVWANEAWGSYWNWDPKETWAFITWTLFAIYLHTRTNKNWESFNCAIVASIGFLIIWICYFGVNLLGIGLHSYGSFNLH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should contain a Helical membrane-spanning region.2. The ideal protein would be one that plays a critical role in cytochrome complex assembly through heme binding within chloroplast thylakoid membrane. The designed protein sequence is ",
    "response#1": "SNLYESLCFLAWACTLTQLLVERSWPSPIVAAAATPMGLGCIAFASFALPDQLQSAAPLVPALRSSWLVMHVSVIMVSYAALLVGSLLSLAVLLTDRGEALELRSSSIGSGGFRQSMRVGSDGVLQLQSIRLSTGEQLDSLSYRTITVGFLMLTVGIVSGAVWANEAWGSYWSWDPKETWALICWLVYAAYLHTRLSRGWQGRRPALVAVVGLVVIAVCYIGVNLLGIGLF",
    "response#2": "LSNLYESLLFLTWTLLTIYLYVEFKTKSKLVGAILIPVALLINGFANLTLSVDMQKSSPLVPALQSNWLMLHVSMMMLSYGTLIMGSLLCILFLVISKYQDIDLQILDESSLPLYNIMLDYYEAKLFSPSDEVSELGKLKLLQSLDNWSYRIIGLGFPFLTIGIIAGGVWANEAWGSYWSWDPKETWALITWIVFATYLHSRITKGWEGKKTAILGGLGFFVIWICYLGVNFLGKGLA",
    "response#3": "FPIYAQQNYESPREATGRIVCANCHLAKKAVDIEVPQAVLPDTVFEAVVKIPYDTQIKQVLSNGKKGGLNVGAVLILPEGFELAPSDRIPPELKEKISNIYFQPYSPEKKNILVVGPLPGNKYSELVFPILSPDPATNKKASFLKYPIYLGGNRG"
  },
  {
    "reference": "MSTLPKPKRQTKRNTLRRPKNVKFPAGGQIVGEVYVLPRRGPQLGVREVRKTSERSQPRGRRQPTPKARPREGRSWAQPGYPWPLYGNEGCGWAGWLLPPRGSRPSWGQNDPRRRSRNLGKVIDTLTCGFADLMGYIPLIGAPVGGVARALAHGVRALEDGVNYATGNLPGCSFSIFLLALFSCLTCPASSLEYRNASGLYLLTNDCSNRSIVYEADDVILHLPGCVPCVETDNNNTSCWTPISPTVAVKHPGVTTASIRNHVNMLVAPPTLCSALYVEDAFGAVSLVGQAFTFRPRQHKTVQTCNCSIYPGHVSGHRMAWDMMMNWSPAIGLVISHLMRLPQTFFDLVVGAHWGVMAGLAYFSMQGNWAKVVIVLIMFSGVDATTHTTGGSAAQATAGFTSFFTRGPSQNLQLVNSNGSWHINSTALNCNDSLNTGFIAGLFYYHKFNSSGCPERMSSCKPITYFNQGWGPLTDANINGPSEDRPYCWHYPPRPCNITKPLNVCGPVYCFTPSPVVVGTTDIKGLPTYRFGVNESDVFLLTSLRPPQGRWFGCVWMNSTGFVKTCGAPPCNIYGGMKDIEANQTHLKCPTDCFRKHHDATFTRCGSGPWLTPRCLVDYPYRLWHYPCTVNFSIFKVRMFVGGHEHRFSAACNWTRGERCDLEDRDRSEQQPLLHSTTDSLILPCSFTPMRRLSTGLIHLHQNIVDVQYLYGVGSAVVGWALKWEFVVLVFLLLADARVCVALWMMLLISQAEAAMENLVMLNALSAA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must bind to  in order to perform its enzymatic function.2. The designed protein localizes to the host cell perinuclear region of cytoplasm. The designed protein sequence is ",
    "response#1": "VGTVGITSYAANALGEVVFVELPEPETTVSVGDGIGAVESVKSASDVYSPVSGTVTSINESLGDSPDKVSSSPEEEGWICKIL",
    "response#2": "VGTVGITSYAANALGEVVFVELPEPETTVSVGDGIGAVESVKSASDVYSPVSGTVTSINESLGDSPDKVSSSPEEEGWICKIK",
    "response#3": "VGTVGITSYAANALGEVVFVELPEPETTVSVGDGIGAVESVKSASDVYSPVSGTVTSINESLGDSPDKVSSSPEEEGWICKIK"
  },
  {
    "reference": "MSFRLLSSLSAKLRSVVYRAATVALIDMKKAQVVFVLGGPGSGKGTQCVRIVENYGYTHLSAGDLLRAERSREGSEFGQLIDNHIKDGKIVPFEITIKLLKKAMEETRRLDEKKIHFLIDGFPRNEENLQGWTTIMDGEADVKFVLFFDCSNEVCINRCVERGKSSGRTDDNRASLEKRIQTYLQSTRPVISLYEKQGKVRTVDASCAVDKVFANVKTILDKEI",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have ATP binding, cytidylate kinase activity within cytoplasm to regulate 'de novo' pyrimidine nucleobase biosynthetic process, pyrimidine nucleotide biosynthetic process, phosphorylation.2. The protein should have a high affinity for CMP.3. We are looking for a protein with broad substrate specificity for a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-   triphosphate + ADP.4. The protein must exhibit the following characteristics: Plays an important role in de novo pyrimidine nucleotide biosynthesis; Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. The designed protein sequence is ",
    "response#1": "ECTLAMIKPNTALLHQDKILAIVAAQGYKVVCKSQATLTEGQASEFYGEHRGKPFFPALLQYMTSGETVALLLQREGAIKGWRALMGPTNSLKAREEAPQSIRGMFGVDGTMNATHGSDAPLSAAREVMFHFR",
    "response#2": "ECTLAMIKPNTALLHQDKILAIVAAQGYKVVCKSQATLTEGQASEFYGEHRGKPFFPALLQYMTSGETVALLLQREGAIKGWRALMGPTNSLKAREEAPQSIRGMFGVDGTMNATHGSDAPLSAAREVMFHFR",
    "response#3": "ECTLAMIKPNTALLHQDKILAIVAAQGYKVVCKSQATLTEGQASEFYGEHRGKPFFPALLQYMTSGETVALLLQREGAIKGWRALMGPTNSLKAREEAPQSIRGMFGVDGTMNATHGSDAPLSAAREVMFHFR"
  },
  {
    "reference": "MEEQQLGAALDKSAAELSVMDVYDIAAALGLELERVIERTGAELLSRLVPRVVRVLELLEVLVSRSSSSPDTDELRLELDRLRLERLERLEKEKKHKKELELVEDVWRGEAQDLLCQISQLQEENKTLLNNLSIRESPLTEEDIQKQEGMTERERQVMKKLKEVVDKQRDEIRAKDRELTLKNDDVEALQQQMSRLMKINQDVRHRVSVVEAQGKSLIQQKVELEAAAQTQQQEVSSLRQEVSRLKEKLKEQSRSNEEEAQEPVGPPSPAQEALCDEDLSTVDLKDPNRPRFTLQELRDVLHERNELKAKVFMLQEEIAYYRSEEQEEENGPPLPDPSETLRTNPRSNFQPESGIKRLFSFFSRDRSVSQRRMMLNVEPVGDAVGSWTGKQEDVYTETAQEALQHM",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.2. Incorporate a signal peptide in the protein design.3. For general function, the protein need meet that Neuroprotective protein .; Plays a role in the regulation of cell shape and polarity; Plays a role in cellular protein transport, including protein transport away from primary cilia4. The designed protein should have protein dimerization activity within cilium, centrosome to regulate epithelial cell morphogenesis.5. The designed protein should contain one or more RH2, RH1 domains that are essential for its function. The designed protein sequence is ",
    "response#1": "GNLVIVITLYRKSYLLTLSNKFVFSLTLSNFLLSVLVLPFVVTSSIRREWIFGVVWCNFSALLYLLISSASMLTLGIIAVDRYYAVLYPMAYPMKITGNRAVMVLAYIWLHSLIGCLPPLFGWSSVEFDEFKWMCVAAWHREPGYTAFWQIWCALLPFLVMLVCYGFIFRVARVKARKVHCGAVVTVEVGVQRTGRKNSSTSTSSSGSRKSAFQGVVYSANQCKALVTILVVIGAFMVTWGPYMVVITSEALWGKNCVSPTLETWATWLSFTSAICHPLIYALCNATFKKTFKHLLHC",
    "response#2": "GNLVIVITLYRKSYLLTLSNKFVFSLTLSNFLLSVLVLPFVVTSSIRREWIFGVVWCNFSALLYLLISSASMLTLGIIAVDRYYAVLYPMAYPMKITGNRAVMVLAYIWLHSLIGCLPPLFGWSSVEFDEFKWMCVAAWHREPGYTAFWQIWCALLPFLVMLVCYGFIFRVARVKARKVHCGAVVTVEVGVQRTGRKNSSTSTSSSGSRKSAFQGVVYSANQCKALVTILVVIGAFMVTWGPYMVVITSEALWGKNCVSPTLETWATWLSFTSAICHPLIYTLRNKDVKAALRRMVR",
    "response#3": "GNLVIVITLYRKSYLLTLSNKFVFSLTLSNFLLSVLVLPFVVTSSIRREWIFGVVWCNFSALLYLLISSASMLTLGIIAVDRYYAVLYPMAYPMKITGNRAVMVLAYIWLHSLIGCLPPLFGWSSVEFDEFKWMCVAAWHREPGYTAFWQIWCALLPFLVMLVCYGFIFRVARVKARKVHCGAVVTVEVGVQRTGRKNSSTSTSSSGSRKSAFQGVVYSANQCKALVTILVVIGAFMVTWGPYMVVITSEALWGKNCVSPTLETWATWLSFTSAICHPLIYCLVNKEVRSALLRVLRC"
  },
  {
    "reference": "MIWRQKTLGKSVTMKGTGLHTGVEAVMTLEPAPVNTGLVFVVPGPDGEVEIPARVEYAVARDQMNRATSLEKDGARVTTVEHILAALYGMGVTNCRIGLEGGEPPVTDCASGRKYVEMVDEAGVVDQGIPALSYHVKRWRRWREGDIQISVEPADGFLLSFHIDYDDPAIGIQDATFSIQPDVFREEIAPARTFALMKDVAALQEMGLAKGGNPGNALVFDNGKLAGDQVLRFPDECVRHKILDLLGDLALLGMPLQGHVHARCSGHDANVEFVRQLAQSERSLPRIYPPRDPQGWDIGSIMEIMPHRYPFLLVDRIISLVPGESVTGLKNVTINESFFQGHFPGHPVMPAVLIIEAMAQTGGVLLLSSVDDARGKLVYFSGIDGARFRRPVTPGDQLRFELEMVKLRGPMCKMHGTAYVDSVKVAEADLMSTIVDT",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Involved in unsaturated fatty acids biosynthesis; Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.2. The Zn(2+) binding site should be stable and able to withstand changes in environmental conditions.3. We require a protein that can specifically bind to the metabolites produced in the lipid IV(A) biosynthesis, lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 2/6, Glycolipid biosynthesis pathway.4. The protein's Proton donor, active site must be carefully engineered to ensure that the amino acids involved in a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine +   H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "MNDKYIASLSDLKALLKSRELTSXQXTXAXRXAXAXAXHXGXDXXIXDXGIXLPADKAXGLPXILXDXKKXXAXAXSXIAXFXAXEKTXIFXXDSXXAXAXHXMMAXQXPXXXEXSXIVXAYXNXPXQSXNNXYXLXEXXAXXT",
    "response#2": "MGTTGPLSLSELGLTARGGREARVXGXAXDSRKVGPGDGFIAWPGAAVDGRXFVADALARGAAAIVCERAEPXGXPFVLVDDARKALAXIAAXFXGXPSXRXAXIAXT",
    "response#3": "MTQNPTTDRPADPTPAADPTSVPVPTSPAPVGASAAVGASAPAGAAAASGAAAASGAAAASGAAAGSGAAAASGAAGASGAAGASGAAGASGAAGASGAAGASGAAGASGAAGTSAAGEVGAAGTAGASGAAGAAGGSEAAGEVGAAGAAGAAVGGSPAAGVAAAGAAVGGSPAAGAAAAGGAASGAAAGAAAGAAAGAASGAAAGAAAGAAAGVAAGVAAGAAAGAAAGAAAGAAAAAGGAAAGAAAGAAAGVAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAEGAAAGVAAGAAAGAAAGAAAGVAAGVAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAEAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAGAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAGAGAAAG"
  },
  {
    "reference": "MAQGSNYKVPFRRRREGKTDYAARMKLVDYDKSRLVVRLSNAHATVQVIDYAPEGDITLASAVSKQLANYGYLGGASNTSAVYLTGYLCAKRALAKGVDSAILDIGLKSAIKGSKVFAALKGAVDAGLDIPYGEAVLPDESRINGEHVANYAESLDDDEIAKLFSKYLERGLQPADLPKNFEETKKNIDEAEE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit 5S rRNA binding.2. For general function, the protein need meet that This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. The designed protein sequence is ",
    "response#1": "MKFLLP",
    "response#2": "XKAN",
    "response#3": "MIPAQTRTEIGKGSSRRLRREGKVPAVIYGVGKEPVSIVFDHKDIINIQANDDFYTSVLTIVLDGKEVNVRAQAMQRHVFKPMIDHVDA"
  },
  {
    "reference": "MPNPLANKRIILGVTGSIAAYKAAEIASRLTQIGAFVDVILTESATKFITPLTFQSVTGRKAYVEADLWGGEGHVTHVGLGRAAELLVIAPASANTISRLAHGFGDNLLTLTALASHCPLLLAPAMDAGMFEHPATQASVKLLKDRGAVFIGPAEGHLASGLVGPGRMVSPAEVIAAARWALSRKGPLAGKKVVVTAGGTQEPIDPVRLVTNRSSGKQGYAVAWAAQDAGADVTLITTVDLPAPCFAKIIAVQTADQMHREVKKATADADLLVMAAAVADFRPARTATDKIKKEKGLPEIEMEPTIDILQSIGEEREKHGWPKRVVGFAAESRDLLDNAAVKLQRKRLDMIVANDISRPDAGFEVDTNRVTFLFPDGTAIPLPKISKEEVAEAVIDRTLVWFKNP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a low Km value for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2. The designed protein sequence is ",
    "response#1": "LEGTHVVITAGGTREALDPVRVLTNHSSGKQGAALARAAYVRGASLSLVLAGSHIEPPAGVDVVRVESAKELAAAVRERESSADVLIMAAAVSDFTPEPSRSKIKKKAGESGLTLHLMETEDILRGLVERRQAGERPFVICGFAAETGDEHSSALAHATRKARAKGADLLAFNDVSENAFGADTNALTVLVGVTGCIAAYKAGEIVRGLQKAGVRVKVVMTEHGTHFVDPVTFRALTHEKVAVDLFDDPSDPIHHISLAQECDVFLIAPCTANVMAKVACGIADDLLSTTALATTATLAIAPAANVHMYEAAATQENMATLRRRGVRFIEGGAGYLACGDVGRGRLADPAVIVRETLG",
    "response#2": "LEGTHVVITAGGTREALDPVRVLTNHSSGKQGAALARAAYVRGASLSLVLAGSHIEPPAGVDVVRVESAKELAAAVRERESSADVLIMAAAVSDFTPEPSRSKIKKKAGESGLTLHLMETEDILRGLVERRQAGERPFVICGFAAETGDEHSSALAHATRKARAKGADLLAFNDVSENAFGADTNALTVLVGVTGCIAAYKAGEIVRGLQKAGVRVKVVMTEHGTHFVDPVTFRALTHEKVAVDLFDDPSDPIHHISLAQECDVFLIAPCTANVMAKVACGIADDLLSTTALATTATLAIAPAANVHMYEAAATQENMATLRRRGVRFIEGGAGYLACGDVGRGRLADPAVIVRETL",
    "response#3": "LEGTHVVITAGGTREALDPVRVLTNHSSGKQGAALARAAYVRGASLSLVLAGSHIEPPAGVDVVRVESAKELAAAVRERESSADVLIMAAAVSDFTPEPSRSKIKKKAGESGLTLHLMETEDILRGLVERRQAGERPFVICGFAAETGDEHSSALAHATRKARAKGADLLAFNDVSENAFGADTNALTFL"
  },
  {
    "reference": "MPQDKIVIHGAREHNLKNIDVEIPRDKLVVVTGVSGSGKSSLAFETLYAEGQRRYVESLSAYARQFLGNMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTVTEINDYLRLLYARVGTPYCVNGHGKISAQSVEEIVEQILELPEKTRLQILAPVVRTKKGTHVKMFERIQKDGYVRVRVDGEVYDISEVPELDKNKKHNIEIVIDRIVVKEGIRSRLFDSVEAALHQAEGYVIVDKMDGSELLFSEFYACPVCGFTVPELEPRLFSFNAPFGSCPDCDGLGVKLEPDVDLLIPDTSKTLREGAIIYWYGKASTYYPALLEQAMEQFGIDLDRPWEKLSEKEQQIVLYGNGDKLFHFLHEGDFGLRDQDMTFVGVIPNLWRRYRSGMSESAREMARSYMTELTCTTCHGYRLNDQALSVKVGEKNIAEFSILSIGDTLDYVKSLVLSANNEIIAKPILKEIKDRLTFLKNVGLDYLTLSRSSGTLSGGESQRIRLATQIGSNLSGVLYILDEPSIGLHQRDNDRLIESLQKMRDLGNTLIVVEHDEDTMMAADWLIDVGPGAGDLGGEIIASGTPKQVMKNKKSLTGQYLSGKRAIPVPEKRRAIDKKKMVKITGASENNLQNLDVEFPMGVMTAVTGVSGSGKSTLVNSILKKSLAQKLNHNSEKPGKHKKITGYEGIERLIDIDQSPIGRTPRSNPATYTSVFDDIRDLFANTNEAKIRGYKKGRFSFNVKGGRCEACSGDGIIKIEMHFLPDVYVPCEVCHGRR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein with ATP binding, zinc ion binding, DNA binding is needed to enhance nucleotide-excision repair, SOS response in a specific context.2. For general function, the protein need meet that A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities3. The presence of ABC transporter domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "KGFEEGKKEQILLGATGTGKTFTMSNIIANLNKPTLILSHNKTLAGQLYGEFKEFFPHNAVEYFVSYYDYYQPEAYVPSTDTYIEKDSAINDEIDKLRHSATSALLERNDVIVVASVSSIFGLGDPHEYKNHVLSLRTGMTIDRNTLLRQLVDIQFDRL",
    "response#2": "DRGDVVIRKHPFEGILNNMARRYKETESMSVREELAKNISNRPCIDCGGSRLRPEARNVYIGRTNLPIIAEKSIGETLEFFTALSLTGQKAQIAEKILKEIRERLQFLVNVGLNYLSLSRSAETLSGGEAQRIRLASQIGAGLVGVMYVLDEPSIGLHQRDNERLLNTLIHLRNLGNTVIVVEHDEDAIRAADHIIDIGPGAGVHGGQVIAQGNADEIMLNPNSITGKFLR",
    "response#3": "KGFEEGKKEQILLGATGTGKTFTMSNIIANLNKPTLILSHNKTLAGQLYGEFKEFFPHNAVEYFVSYYDYYQPEAYVPSTDTYIEKDSAINDEIDKLRHSATSALLERNDVIVVASVSSIFGLGDPHEYKNHVLSLRTGMTIDRNTLLRQLVDIQFDRV"
  },
  {
    "reference": "MTDSSSAGSTPAISEVHPDRLAAWLTARGERAYRARQILAGSHRPGVSDFGDLTELPRALRSALEESFTFSTIRHSHALVADGGLTAKAVHELGDGQRIESVLMRYPRRSGGQRTTICISSQAGCAVGCPFCATGQAGFARQLSAGEIVDQVLHWHRAPWLALGPDWRPGTGAGHYNIVFMGMGEPLNNTDRVFGAVRLLNDPDRLGIGARHITVSTSGVVPGIERMIGELPQVNLAISLHAADDGLRDELVPINRKWPIADVVGAGRRFAEVTRRRVSLEYVLIDGVNDSIRLAERLAALAAGWLCHVNLIPLNPTPGSRWSGTPPAGIAAFAGVLRAARVPVTVRDTRGREIEAACGQLHAQLAGRELPRPSLVAIGGASLG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a binding site that is specific to the target [4Fe-4S] cluster ligand.2. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.3. A protein that contributes to rRNA base methylation by functioning in cytoplasm with rRNA binding, tRNA binding, 4 iron, 4 sulfur cluster binding, metal ion binding, tRNA (adenine-C2-)-methyltransferase activity.4. The protein with the reaction adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine, the S-methylcysteine intermediate, Proton acceptor must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MNIYGMTRYDLEQYFINNNMHKFKALQVFEWLYIHKVKSFFEMTNIKKELQEQLERDFSTRMIHIEKKQQDQETSKYLFKLFDNNYVEAVLMKHDYGLSVCVSSEVGCNMGCAFCESGRLKKVRELNSYEMVEQILLIEQDIGKRVSSIVIMGIGEPLDNYDNVMKFIRIINDPKGLAIGARHITLSTCGLVPKIKELSNLDLQINLAISLHAPNNELRSKIMPINKAYNLNTLIDTIKEYIEKTNRRVTIEYVMLDNINDSEKEAKELSSLIKNLNVYVNLIPYNETNHLEFKRTSKNKIMKFYDYLKKQGINVTIRREFGSNIDAACGQLRSKEVEKLKN",
    "response#2": "MNIYGMTRYDLEQYFINNNMHKFKALQVFEWLYIHKVKSFFEMTNIKKELQEQLESQYPRRHLLDLSLAELRDWVVERGEKPFRAAQVWRWIYQGRAADFESMTDLSRSLRQHLAEDFVLFAGSIARHQTSPDGTEKLLIGLADGGEVECVLLRDGPRRSICVSSQVGCAMGCVFCASGLDGVDRNLTRGEILEQMLRLQHRLDHEERLSHIVMMGMGEPLANLERVLSALDVARSPEGLGISPRRITISTVGLPPAIDRLARHGVPYNLAVSLHAPEDELRNRLVPVNRKIGVEALMAAADRYFEASGRRLTFEYVLLGGLNDSPTQAAQLGHLLRGRGAMLNVIPYNPVAGLPYRTPTSTATSRFREVLERGGINVNFRVRKGGEIDAACGQLRRNRQATASLVPL",
    "response#3": "SSQAGCGMNCPFCATGQAGLTRNLSTAEIVEQVLAGSRSLDRGQIAGGPGRVSNVVFMGMGEPMANYGAVVESVRRLISPAPSGLGLSARGITVSTVGLVPRMKDLATEGLPVTLALSLHAPDDELRNTLVPVNTRWPVAEVLNAAWEYADRTHRRISIEYALIRDINDQAGGHPALQWYPDRATLCISSQAGCGMACPFCATGQGGLDRNLSTGEIVDQVRAAAATMQAKGGRLSNIVFMGMGEPLANYNRVLSAIHQITQPSPEGFGISQRGITLSTVGLAPAIRRFAKEGLSCRLAVSLHTPDDELRDSLVPANNRWPVEEVLDAAKEYADASGRRVSIEYALIKDINDQPWRADLLGKKLHGALGAKVHVNLIPLNPTPGSKWDASPKRVQDEFQRRVIARGVTCTVRDTRGQDVAAACGQLRAQQVQG"
  },
  {
    "reference": "MGETIRFGISIDETLLESFDKLIDQKGYMNRSEAIRDLIRASLVELKWEAGEEETVGTVTLVYNHHVRDLSDKLTEHQHTHHDQIISALHVHLDAHNCLEVLVVRGKAREVKQIADELIGVKGVKHGKLVMTTTGEELH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have DNA binding, DNA-binding transcription factor activity, nickel cation binding to facilitate response to nickel cation.2. The presence of Ni(2+) is necessary for the protein to function in the desired environment.3. The protein must exhibit the following characteristics: Transcriptional regulator.. The designed protein sequence is ",
    "response#1": "MGVISLIYDHHQSQLQKKVTDLQHDFHHHIVSTTHVHMDHDNCLEVIIVKGRAGDVIELADGLAALKGVRDSNLAN",
    "response#2": "MGVISLIYDHHQSQLQKKVTDLQHDFHHHIVSTTHVHMDHDNCLEVIIVKGRAGDVIELADGLAALKGVRDSNLAA",
    "response#3": "MGVISLIYDHHQSQLQKKVTDLQHDFHHHIVSTTHVHMDHDNCLEVIIVKGRAGDVIELADGLAALKGVRDSNLAV"
  },
  {
    "reference": "MGGKWSKSSLAGWPAVRERIRRTPPAAERGAAEGVGAVSRDLDRRGAITSSNTGATNPDLAWVEAQEEDGEVGFPVRPQVPPRPMTYKGALDLSHFLREKGGLEGLIYSQKRREILDLWVYNTQGYFPDWQNYTPGPGTRYPLTFEWCFKLVPVDPEEVEKANEGENNCLLHPMSQHGMEDEDREVLKWQFDSRLAFKHEARERHPEFYQD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design must feature the PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif.2. The protein should have GTP binding and be active in membrane, host cell Golgi membrane, virion component. The designed protein sequence is ",
    "response#1": "GFIS",
    "response#2": "MPQLDTSTWFTILFFSFMIITMT",
    "response#3": "LPTGF"
  },
  {
    "reference": "MTTLAIVGATGAVGSVMIDIIDARETVPWSEIRLIASPRSAGRRLRVRGEEIEVVALSPEAFDGVDIAMFDVPDAVSAEWAPVAVSRGAIAVDNSGAFRMDPEVPLVVPEVNAESAHTRPKGIVSNPNCTTLSMMAALGALHRTFGLRELVVASYQAASGAGQEGIDRLQAELSAVAGKGVGNRRGDVAEAIAAAGLPPESPFTAPLALNVVPFAGSLRDGGWTSEELKVRNESRKILGLPDLKVSATCVRVPVVTTHSIAVHATFDGPVDVATAQQVFAAQPSIVLVDEPEAKRFPSPVDVVGEDPTYVGRVRQALDFPNTLDFFVCGDNLRKGAALNTYEIAEELVRA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be able to compete with endogenous proteins or inhibitors for phosphate binding.2. The protein design must be able to perform protein dimerization activity in order to contribute to isoleucine biosynthetic process, threonine biosynthetic process.3. The designed protein must have at least one Semialdehyde dehydrogenase NAD-binding domain.4. The designed protein should interact with enzymes and substrates within the L-threonine biosynthesis, L-threonine from L-aspartate: step 2/5 pathway to enhance or regulate its activity.5. The designed protein must have a Acyl-thioester intermediate that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "LYPLHLEAGLESFTAVTFQSVSGTGKAALEELREGTEAFLSGKAFEPKVYPRPIAFNAIPQIGSFDDEGISQEEWKMVNESRKILGIPDLAVSCTTVRVPVFRGHSEAITAIFRTPLSPERARAILKEAPGVALAGEAGPDSYATALDVPGTDAVVVGRIRRDTGSGSGLSHWVVADNLRR",
    "response#2": "LKPVKDAAGIRRVVVSTYQAVSGAGKEAIDELADQTRTVLNGGEYTPKVFPYQIAFNLIPHIDVFQEMDYTKEEWKMVKETRKILHDENMAITATTVRVPVYRSHSESINIETEKKLTAAGVKELLARFPGVVVMDEPAAKKYPMPLYCSDRDEVFVGRIREDNSVDCGLNLWVVADQLRH",
    "response#3": "LKPVKDAAGIRRVVVSTYQAVSGAGKEAIDELADQTRTVLNGGEYTPKVFPYQIAFNLIPHIDVFQEMDYTKEEWKMVKETRKILHDENMAITATTVRVPVYRSHSESINIETEKKLTAAGVKELLARFPGVVVMDEPAAKKYPMPLYCSDRDEVFVGRIREDNSVDCGLNLWVVADQLRA"
  },
  {
    "reference": "MGDFIGHISPGLFLVFYGLYQAIIVSRALILNDSLLYPSYLSKNKGKWARLWQIAHAGWLKVVSGSLLIVYELNCVDEGLTFMTKMIPPRFMYPKEWQHLTMFILLTLDGCVEVVSRSVLRQRLVLLERGATVLGVYVLLLLLVSHVKDSSGVELQVHSLLILVVFLLMLVLTAELWAPEMVHLWVIETFLFLTMGSWLMQAAFILFRPVSGFPWEDDDISNIMLVTTFFCWHVMINALCMLGIYGISSFWHRCYRTGLMPMGSKEVLYHKSSEGTFYKLLQKAEQQDRDDQAPLLSKSSPCDRA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "GFPGF",
    "response#2": "MPN",
    "response#3": "MNKENN"
  },
  {
    "reference": "MQEKAVHIQKEVIEVNLSKKDIRKMSIDAIAEDLTASGEKAFRAKQIYEWLWMKSAASFEEMTNLSKNLREWLDQNYCINRITIADKQLSSDRTIKVAFRLHDGNEVEGVLIPTENRMTACVSSQVGCSLSCKFCATGYLKRMRNLEAAEIYDQVVMIKELAETHYDMPLSNIVYMGMGEPLLNYKNMMESIEHITSEKGLHMSPKRITVSTAGISKMIKKLADDDAKFNLALSLHAANDEKRSQIMSINDSNNLPVLREALEYYHSKTKNRVTFEYCVFNNFNDSLEDAKELWQFTKYVPAKVNLIEYNPIDQADFTNTDEDKLDKFAAFLEDRGVIVNVRRSRGKDIDAACGQLANKH",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have rRNA binding, tRNA (adenine-C2-)-methyltransferase activity and be active in cytoplasm.2. The designed protein should be able to compete with endogenous proteins or inhibitors for S-adenosyl-L-methionine binding.3. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor, S-methylcysteine intermediate to enable effective catalysis of adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "SSQAGCGMNCPFCATGQQGLTRNMSAAEIVEQVVAAERMLAGGEIAGGTGRVTNVVFMGMGEPLANYKAVVTAVRRMTDPAPSGLGIGRRGVTVSTVGLVPAIDKLAAENLGVRLALSLHAPDDELRDELVPVNTRWKVGEALDAARAYFEKTGRRVSIEYALIREINDHGYEQSNQLEADRRTVCISTQVGCGMGCVFCASGLKGVERNLSTGEILEQVLRLRNLLPPDDRLTNLVVMGMGESLANLDRLIPALDRLCSPEGLNFGQRRVTISTVGLPEKMRKLAELDRQYHLAVSLHAPTPRLRDELVPINAKVGLDAVMQAADAYFHRTGRQVTFEYVLLADLNDRPEDAEALGALLSGRKAHVNLIPYNPVAGLPYRRPSAEAVRRFERIVSGRGVSVSVRKTKGREIDGACGQLRRGAA",
    "response#2": "VTLCVSSQAGCGMNCPFCATGQQGLTRNMSAAEIVEQVVAAERMLAGGEIAGGTGRVTNVVFMGMGEPLANYKAVVTAVRRMTDPAPSGLGIGRRGVTVSTVGLVPAIDKLAAENLGVRLALSLHAPDDELRDELVPVNTRWKVGEALDAARAYFEKTGRRVSIEYALIREINDHAWRGDLLGKLLNERGRGWVHVNPIPLNPTPGSKWTASKPEDEAAFVAASRVVLSP",
    "response#3": "NTVCVSSQVGCPMGCLFCATGKLGFKRNLTADEIVEQVLYFARQLKKENKRVTNIVFMGMGEPMVNYDNVMAAIRILNNPNGFNLGARRMSISTSGIPEGIRKFAREGLEVNLALSLHAPNNELRCKIMPVSRRHSLEEVMDALREYVDITHRKIMFEYIMIKDVTDTPELAHELVKLLSGWLGYVNLINYNQTEMFAPSTRERIEEFKQYTGYDSVLEVQSVLERGGEPAYRLRQAYAALTGSLVRDWEEATSLPQGLRGALNQEAPAAVLDLRRTWRATDGTRKYLFHTHDGHAIETVMIPEKGRHTVCISTQVGCAMACKFCATGLLGIKRNLGAREIAEQVFVCARDISPERVSNVVVMGMGEPMLNYKATLGALKVLNDPKGFGLAARHIAVSTSGLVDKIRRFADEPEQFHLAISLHTPFEEERREIMPVAARHTIPELMDAARYFVSRTRRKLFFEYTLLAGVNDQPRHVEALAELFDHPLYHLNLLRFNWTDTGFSATSKADAKRFLNHARDLGLSATLRPSRGQDIDAACGQLAARDESYPPT"
  },
  {
    "reference": "MLRNMFKKTYTKIDTKYKQTGGPGEEPVIPEGLWRKCNKCGQPIYVEDVKNNYYVCPKCSGYFRVHAYRRIEMLVDEGTFEEWNKEMPFSNPLEFPGYEKKVLAAREKSRLNEAIVTGKGLIDGNPAVIGVCDARFIMSSMGHVVGEKITDAVERATKEKLPVIIFACSGGARMQEGIVSLMQMAKTSAALKRHHDAGQLFISVLTDPTTGGVTASFAMLGDIILAEPHALIGFAGPRVIEQTIGQKLPEGFQRSEFLLDHGFVDKIVEREDQKRVIGQILYMHRSHKMNIQIPVDRNHVGVDKSMENSGDSGKTAWDTVLLSRKSDRPVAADYINAIFDEFIEFHGDRYFGDDGAVMGGIAMFHGMPVTVIGQQKGKNTKENLKRNFGMPSPDGYRKALRLMKQAETFGRPIICFVDTPGAFCGLEAEERGQGEAIARNLFEMSDLKVPVLSVVIGEGGSGGALAMAVADEVWMLENAIYSILSPEGFASILYKDSKKAPDAAKVMKVTAQDLKKLGLIERIVPEEEPVCADNLYRVAEFMDSAMAEFFESYLAMSGEELAEHRYQRFRRM",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein have enhanced acetyl-CoA + N(6)-carboxybiotinyl-L-lysyl-[protein] = malonyl-   CoA + N(6)-biotinyl-L-lysyl-[protein]. The designed protein sequence is ",
    "response#1": "VLGAKDLPRTILSDHIEQRLFKRLKQERTERARVQGTSYDEIPTVESLVVRVVSSVDKKLEVKSRFLEIFREDNFPTEFPYKSKVVLLFQKIEGVEVCLFGMYVQEFGSECSNPNQRRVYLSYLDSVKYFRPDIKSANGEALRTFVYHEILIGYLEYCKLRGFTSCYIWACPPLKGEDYILYCHPEIQKTPKSDKLREWYLAMLRKAAKEGIVAETTNLYDHFFLQTGECRAKVTAARLPYFDGDYWPGAAEDIISQMSQEDDGRKGNKKGILKKPITKRALKASGQSDFSGNASKDLLLMHKLGETIHPMKEDFIMVHLQHSCTHCCTLMVTGNRWVCSQCKDFQLCDGCYEAEQKREDRERHPVNQKDKHNIFPVEIADIPTDTKDRDEILESEFFDTRQAFLSLCQGNHYQYDTLRRAKHSSMMVLYHLHNPTC",
    "response#2": "VLGAKDLPRTILSDHIEQRLFKRLKQERTERARVQGTSYDEIPTVESLVVRVVSSVDKKLEVKSRFLEIFREDNFPTEFPYKSKVVLLFQKIEGVEVCLFGMYVQEFGSECSNPNQRRVYLSYLDSVKYFRPDIKSANGEALRTFVYHEILIGYLEYCKLRGFTSCYIWACPPLKGEDYILYCHPEIQKTPKSDKLREWYLAMLRKAAKEGIVAETTNLYDHFFLQTGECRAKVTAARLPYFDGDYWPGAAEDIISQMSQEDDGRKGNKKGILKKPITKRALKASGQSDFSGNASKDLLLMHKLGETIHPMKEDFIMVHLQHSCTHCCTLMVTGNRWVCSQCKDFQLCDGCYEAEQKREDRERHPVNQKDKHNIFPVEIADIPTDTKDRDEILESEFFDTRQAFLSLCQGNHYQYDTLRRAKHSSMMVLYHLHNPTC",
    "response#3": "VRGAKDLPRTLLSDHIEERLFKRLREERQERANKLKTSLDEVPGADGLVVRVVSSVDKKLEVKPHFFKILQEDNYPAEFPYKSKAILLFQKIEGVEVCLFGMYVQEYGAECKFPNQRRVYLSYLDSVKYFRPDIETVSGQALRTYVYHEILIGYLEYYKQRGFTSCYIWACPPVKGEDYILYCHPEIQKTPKSDKLRQWYLSMLQKAIKENIVVELTNLYDQFFVTAKECKIKVSAARLPYFDGDYWPGAAEDIINQLQLEGDGKLLKKGRVNKIITKRALKAAGHTDLSGNASKEAMLMQKLGEIICPIKDDLIMVHLQYSCSHCCTFMVSGRRWVCNECKSFYICDRCYNAEQRLEEKERHPSNSKCLHILHPVEIVGVSEDTKDRDIILENEIFDTRQAFLSFCQGYHYQYDTLRRAKHSTMMMLYHLHNPTCGRCHHLSSAKAKYQKRFSYAETDAEAEWWVQCDKCKAWQHQICALFNPKIVDPEAEYTCAKS"
  },
  {
    "reference": "MFSSIEEALEDLKAGRPVIVTDDEDRENEGDLLAIAELADAETVNFMAKHGRGLICMPITEEKAAALQLPLMVAENTDSYGTAFTVSIDHSTTTTGISAGERSETIRRAMDQDAVPGDFRRPGHVFPLIAKAGGVIQRPGHTEAAVDLARLAGHEPAGVICEIMNDDGTMARLPELTVMAEEFGLKLITIEQLIEYRKKHDRLVSREAEIRMPTDFGEFRMIGYTERLTGQEHLALIKGEIRAGSAVPVRIHSECMTGDVFGSRRCDCGPQLHAALRQIEEEGEGVLLYMRQEGRGIGLINKLKAYRLQEEGLDTVEANERLGFPDDLRDYAIAAQMLKDLGIGEVRLMTNNPRKVDGLAKYGIRITERLPIEMEPVKENQRYLNTKMEKLGHLLHV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have zinc ion binding to facilitate riboflavin biosynthetic process.2. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+), D-ribulose 5-phosphate binding.3. A protein should be able to manipulate the activity of enzymes in the riboflavin biosynthesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "DEADLPTEHGRFRIAVFEDVIHHQDHVALIRGEIDSDQPILVRAHSQCLTGDTFGSLRCDCGDQLRRAMDMIDKEGRGVILYILNHEGRGIGLTNKIKAYAIQDQGADTVEANRKLGFLADQRDYGLGAQILATLGVRKIRLLTNNPRKFIGLAGYGLEIVERVD",
    "response#2": "DEADLPTEHGRFRIAVFEDVIHHQDHVALIRGEIDSDQPILVRAHSQCLTGDTFGSLRCDCGDQLRRAMDMIDKEGRGVILYILNHEGRGIGLTNKIKAYAIQDQGADTVEANRKLGFLADQRDYGLGAQILATLGVRKIRLLTNNPRKFIGLAGYGLEIVERVR",
    "response#3": "RVETIPLKNEFGDWLLLAYRARTDHATHVALCRGGIGELDARGSVVPADEPVLVRVHSECLTGDVFGSMRCECGEQLRAAMRLIAEAGRGAVVYLRQEGRGIGLESKLHAYRLQDQGLDTVEANQFLGFRDDHRDYGIGAQILRDLGVTRLRILTNNPKKISRLEVYGLHIVEQLL"
  },
  {
    "reference": "MPHKVRLKGCAEELASGVGLGESESLPGFSRRRRPSLRTPGQRRGPRPGRGLCSSRRASERARAANAPGRDPAARMTARGAAGRCPPTTWLCPWLLLFCLLVSRTITEEASENCSHLIGNGHLRFLQELIDSQMESLCEISFRFVDPDQLKDPVCYLKKAFPLVQDIMEDTMRFKDNTHNANVTMQLQELSLRLKSCFTKDYEEQDKACIRTFKETPLQLLAKIKNVFNEIKNLLKNDWNIFSKNCSNDYANCPSPDVVTKPDCNCLYPKGTPSSDLASVSPQQPLTPSMAPMTGLTWADSEGTEGSSLLPSEQPLRTVDPGSAKQRPPRSTCQSFELSESPGMEGSPATGSPQPRPSVGAPVPGTEDVLDSVLDPNWALEEASGVASEGPVPQEAELSSSRLEGGSVQAETTRPSDLLSASSAFSGLAKGRQPVDVTGTPVPKVGPVRPTGRAQSYRPEKTDGSSAPPRDHREPGSARTPSLRTQGLSRPSTLSAHPRLPRSHSWGNVLPLGELEGKRSTRDRRSPAELEGGRASERAARPLARFNSIPLTDAGHETKHVGPADPQLPGFVFRLLVPSIILVLLAVGGLLFYRRRRRSHQELQTVDVPMERLEDSPLTQDEDRQVELPV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should emphasize Polar residues, Basic and acidic residues compositional bias.2. The designed protein should have macrophage colony-stimulating factor receptor binding, cytokine activity, protein homodimerization activity, growth factor activity within CSF1-CSF1R complex, extracellular space, perinuclear region of cytoplasm, plasma membrane, nuclear body to regulate osteoclast proliferation, microglial cell proliferation, positive regulation of cell-matrix adhesion, positive regulation of macrophage differentiation, positive regulation of monocyte differentiation.3. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "GPPRDVTASCNSSHCTVSWAPPSTWASLTARDFQFEVQWQSAEPGSTPRKVLVVEETRLAFPSPAPHGGHKVKVRAGDTRMKHWGEWSL",
    "response#2": "NLTCEIRAARFLSCAWREGPAAPADVRYSLRVLNSTGHDVARCMADPGDDVITQCIANDLSLLGSEAYLVVTGRSGAGPVRFLDDVVATKC",
    "response#3": "LQFGKTLGAGAFGKVVEATAFGLGKEDSVLKVAVKMLKSSADTDEQEALMSELKIMSHLGHHENIVNLLGACTYGGPILVITEYCRYGDLLNFLRRKAESIIIQDSSLDTSLDSTADYKNIDLEKKYIRSDSGFASQGLETYVEMRPVSSSSSSASSDSAQGRGKSSEEEVETREDLRPLNLADLLQFSSQVAQGMAFLASKNCIHRDLAARNVLVSDGRVAKICDFGLARDIMNDSNYVVKGNARLPVKWMAPESIFDCIYTVQSC"
  },
  {
    "reference": "KDLAAMLRIINARKEKKRRGADTSVGIVGLLLTTAMAAEVTRRGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHMCDATMSYECPMLDEGVEPDDVDCWCNTTSTWVVXXXCHHKKGEARRSRRAVTLPSHSTRKLQTRSQTWLESREYTKHLIRVENWIFRXXXXXXXXXXXXWLLGSSTSQKVIYLVMILLIAPAYSIRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKXXSYSLCTAAFTFTKIPAETLHGTVTVXXXXAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMXELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRMAVLGDTAWDFGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLALGGVLIFLSTAVSADVGCSVDFSKKETRCGTGVFVXXXVEAWRDRYKYHPDSPRRLAAAVKQAWEDGICGISS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The ideal protein would be one that plays a critical role in suppression by virus of host type I interferon-mediated signaling pathway, viral entry into host cell, suppression by virus of host JAK-STAT cascade via inhibition of STAT2 activity, viral RNA genome replication, virion attachment to host cell through double-stranded RNA binding, ribonucleoside triphosphate phosphatase activity, ATP binding, RNA-dependent RNA polymerase activity within host cell endoplasmic reticulum membrane, viral capsid, extracellular region, membrane.2. The protein contains novel RdRp catalytic, Flavivirus NS2B, Helicase C-terminal, Peptidase S7 domains that confer a unique function or activity.3. We are looking for a protein with broad substrate specificity for a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-   diphosphate + H(+) + phosphate.4. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "AGVLWDVPSPPPVGKAELEDGAYRIKQRGILGYSQIGAGVYKEGTFHTMWHVTRGAVLMHRGKRIEPSWADVKKDLISYGGGWKLEGEWKEGEEVQVLALEPGKNPRAVQTKPGLFKTNTGTIGAVSLDFSPGTSGSPIVDRKGKVVGLYGNGVVTRSGAYVSAIAQTEKSIEDNPESGCVVSWKNKELKCGSGIFITDNVHTWTEQYKFQPESPSKLASAIQKAHEEGICGIRSVTRLENLMWKQITPELNHILSENEAKLTIMTGDIKGIMQAGKRSLRPQPTELKYSWKTWGKAKMLSTEPHNQTFLIDGPETAECPNTNRAWNSLEVEDYGFGVFTTNIWLKLKERQDVFCDSKLMSAAIKDNRAVHADMGYWIESALNDTWKIEKASFIEVKSCHWPKSHTLWSNGVLESEMIIPKNFAGPVSQHNYRPGYHTQTAGPWHLGKLEMDFDFCEGTTVVVTEDCGNRGPSLRTTTASGKLITEWCCRSCTLPPLRYRGEDGCWYGMEIRPLKEKEENLVNSLVTAGGKAFEATVRGAKRMAVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLLSGCVVSWKNKELKCGSGIFITDNVHTWTEQYKFQPESPSKLASAIQKAHEEGICGIRSVTRLENLMWKQITPELNHILSENEAKLTIMTGDIKGIMQAGKRSLRPQPTELKYSWKTWGKAKMLSTEPHNQTFLIDGPETAECPNTNRAWNSLEVEDYGFGVFTTNIWLKLKERQDVFCDSKLMSAAIKDNRAVHADMGYWIESALNDTWKIEKASFIEVKSCHWPKSHTLWSNGVLESEMIIPKNFAGPVSQHNYRPGYHTQTAGPWHLGKLEMDFDFCEGTTVVVTEDCGNRGPSLRTTTASGKLITEWCCRSCTLPPLRYRGEDGCWYGMEIRPLKEKEENLVNSLVTAG",
    "response#2": "RCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGASGCVVSWKNKELKCGSGIFITDNVHTWTEQYKFQPESPSKLASAIQKAHEEGICGIRTRCIGISNRDFVEGVSGGSWVDIVLEHGSCVTTMAKNKPTLDFELIKTEAKQPATLRKYCIEAKLTNTTTESRCPTQGEPSLNEEQDKRFICKHSMVDRGWGNGCGLFGKGGIVTCAMFTCKKNMEGKIVQPENLEYTIVITPHSGEEHAVGNDTGKHGKEIKITPQSSTTEAELTGYGTVTMECSPRTGLDFNEMVLLQMEDKAWLVHRQWFLDLPLPWLPGADTQGSNWIQKETLVTFKNPHAKKQDVVVLGSQEGAMHTALTGATEIQMSSGNLLFTGHLKCRLRMDKLQLKGA",
    "response#3": "PGVSCDRFYTLPSKKWETNSPGWEWKLPDALSELGVFGQNIQYHYNYRSGWAVHVQCNATKFHQGTLLVLMVPEHQLGVNETADFNKTMPGKNGRNLEVPYLLDDGTSLANALIYPHQWINLRTNNAATLVFPYANCIPMDSGIRHSNVSLIVVPVVPLKAAAGTTPFVAVTVTIAPMCSEFGGLGKAFEATVRGAKRMAVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLLSGCVVSWKNKELKCGSGIFITDNVHTWTEQYKFQPESPSKLASAIQKAHEEGICGIRSVTRLENLMWKQITPELNHILSENEVKLTIMTGDIKGIMQAGKRSLRPQPTELKYSWKTWGKAKMLSTESHNQTFLIDGPETAECPNTNRAWNSLEVEDYGFGVFTTNIWLKLREKQDVFCDSKLMSAAIKDNRAVHADMGYWIESALNDTWKMEKASFIEVKSCHWPKSHTLWSNGVLESEMVIPKNFAGPVSQHNYRPGYHTQTAGPWHLGKLEMDFDFCEGTTVVVTEDCGNRGPSLRTTTASGKLITEWCCRSCTLPPLRYRGEDGCWYGMEIRPLKEKEENLVNSLVTAGSSIGKAFTTTLKGAQRLAALGDTAWDFGSVGGVFTSVGKAVHQVFGGAFRSLFGGMSWITQGLLGALLLWMGINAFSWPLNEGVMAVGLVSILASSLLRNDVPMAGPLVAGGLLIACYVITGTSADLTVEKAADVTWEEEAEQTGVSHNLMITVDDDGTMRIKDDETENILTVLLKTALLIVSGIFPYSIPATLLVWHTWQKQTQ"
  },
  {
    "reference": "DCASGPCCRDCKFLKEGTICKRARGDNMDDYCNGKTCDCPRNPHKGEHDP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein's functional design necessitates the inclusion of a Cell attachment site motif.2. The designed protein must have at least one Disintegrin domain.3. The protein must exhibit the following characteristics: Has antiplatelet activities on human, followed by human, guinea pig, rabbit and rat platelet-rich plasma.. The designed protein sequence is ",
    "response#1": "PNTDNCCILDGRFGEYCPTTCGISDFLNRYQENVDTDLQYLENLLTQISNSTSGTTIIVEHLIDSGKKPATSPQTAIDPMTQKSKTCWMKLTDMKNYYQYEENILYLQEVYSSNQNKIFLLKQKIANLELQCQQPCRDTVQIQC",
    "response#2": "DAGGCLHADTDMGVLCPTGCTLQQTLLNQERPIKSSIAELNNNIQSVSDTSSVTFQYLTLLKDMWKKKQAQVKENENVINEYSSILEDQRLYIDETVNDNIPLNLRVLRSILEDLRSKIQKLESDISAQMEYCRTPCTVSCNIC",
    "response#3": "DAGGCLHADTDMGVLCPTGCTLQQTLLNQERPIKSSIAELNNNIQSVSDTSSVTFQYLTLLKDMWKKKQAQVKENENVINEYSSILEDQRLYIDETVNDNIPLNLRVLRSILEDLRSKIQKLESDISAQMEYCRTPCTVSCNIC"
  },
  {
    "reference": "MDFLPIFLNLKGRDCVVIGGGEIAARKIGLLLEAGAQVRVYAPALGATLGRLAREGKLRHSAQAFRPEQLDEAVLAIAATDNEAVNRSVYEAAKARRIPVNVVDHPELSSFIMPSIIDRSPVVAAVSTSGASPVLARLIRARLETLIPAGFGRLASLAASVRDQVKSRIRQPERRRVFWEEVLGGYIAELVYSGQDAAARAALQSKLDAVDSDATVTGEVYLVGGGPGDPELLTFRALRLMQQADVVVYDRLVSPEVLNLVRRDAVRIYAGKERDLHTLPQEDINQLLVRLAREGKRVVRLKGGDPFIFGRGGEEIDTLAQEGIPFQVVPGITAAAGCAAYAGIPLTHRDLAQSVVFVTGHLKDGTVNLNWRALAQPRQTVVFYMGLHGVETICRELVRHGLPVATPAALVQQGTTRHQRVLTGTLDTLPSIVDRAGVKPPTLIIVGEVVTMREKLKWFEPDS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein with the reaction NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin, the Proton acceptor must be optimized to ensure efficient catalysis.2. The designed protein should have NAD binding, uroporphyrin-III C-methyltransferase activity, sirohydrochlorin ferrochelatase activity, precorrin-2 dehydrogenase activity to facilitate cobalamin biosynthetic process.3. The S-adenosyl-L-methionine, NAD(+) binding site should be located in a region of the protein that is accessible to the ligand.4. The protein must exhibit the following characteristics: Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1; Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.; Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin.5. The presence of Sirohaem synthase dimerisation, Tetrapyrrole methylase, Siroheme synthase central domains is critical for the interaction of the designed protein with its intended targets.6. Ideal protein should have a binding affinity for key enzymes in the siroheme biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MFARLEGRPVLLVGGGEVALRKARLLLAAGARLTLVSPVLASEFDELAGRFNHLPVRFEPAHLAGQLLVVAATDDNDVNALVYESATELGLFVNLVDDPARSSFIFPSIIDRSPIMVAVSSGGKAPVLVRLLRERLESLLPRHLGGLAELSGRVRHRAKELLSSISDRRRFWERAFASNALAGLIEKQDWQRAEAWLNEAPGRVVLVGAGPGDPGLLTLKGLQQIQQAEVVLYDQLVSPEILDLVRRDATLISVGKKAGDHSVPQDETNRLLVQYAKAGNRVVRLKGGDPFMFGRGGEELEVLADEGIPFSVVPGITAAAGATAYAGIPLTHRDHAQSAVFITGHCQKAGKEPDWQQLAATNQTLVIYMGLMRSEHIQQQLIDHGRQPQTPIAIIERGTQTRQKVLTGTLGELGELVLVGAGPGDPGLLTLKGLQVIQQADVVLYDHLVSAEILELVRRDADKICVGKRAGCHSVAQEETNALIVKLAHQGKKVVRLKGGDPFIFGRGGEELQVGASADIPFQVVPGITAAVGASAYAGIPLTHREHSQSVTFITGHCRENGNELDWPALARGNQTLAIYMGVIKSALISQKLISHGRDASTPVAVIGCGTRSEQQVLTGTLSELERLAQDAPSPALLVVGEVAQLGEVALVGAGPGDPGLLTLRGLQLMQQADVILYDYLVSDDVLELCRRDAEMICVGKRAGFHSVPQEETNALLVKFASRGKRVVRLKGGDPFMFGRGGEELQVVAEAGIPFQVVPGITSAAGATAYAGIPLTHRDHAQSVMFITGHLKKEKDSLDWSTLARGNQTLVIFMGLMKSDHIRDQLIKHGRAAHTPVAIIENGTRSEQRVLKGTVHDLATLAEHASSPALIVVGEVVQL",
    "response#2": "MFARLEGRPVLLVGGGEVALRKARLLLAAGARLTLVSPVLASEFDELAGRFNHLPVRFEPAHLAGQLLVVAATDDNDVNALVYESATELGLFVNLVDDPARSSFIFPSIIDRSPIMVAVSSGGKAPVLVRLLRERLESLLPRHLGGLAELSGRVRHRAKELLSSISDRRRFWERAFASNALAGLIEKQDWQRAEAWLNAVGAGPGDPGLLTLKGLQVIQQADVVLYDHLVSAEILELVRRDADKICVGKRAGCHSVAQEETNALIVKLAHQGKKVVRLKGGDPFIFGRGGEELQVGASADIPFQVVPGITAAVGASAYAGIPLTHREHSQSVTFITGHCRENGNELDWPALARGNQTLAIYMGVIKSALISQKLISHGRDASTPVAVIGCGTRSEQQVLTGTLSELERLAQDAPSPALLVVGEVVGAGPGDPGLLTLKGLQQIQQAEVVLYDQLVSPEILDLVRRDATLISVGKKAGDHSVPQDETNRLLVQYAKAGNRVVRLKGGDPFMFGRGGEELEVLADEGIPFSVVPGITAAAGATAYAGIPLTHRDHAQSAVFITGHCQKAGKEPDWQQLAATNQTLVIYMGLMRSEHIQQQLIDHGRQPQTPIAIIERGTQTRQKVLTGTLGELATLARHAASPSLIVIGEVLARLGGAC",
    "response#3": "MFARLEGRPVLLVGGGEVALRKARLLLAAGARLTLVSPVLASEFDELAGRFNHLPVRFEPAHLAGQLLVVAATDDNDVNALVYESATELGLFVNLVDDPARSSFIFPSIIDRSPIMVAVSSGGKAPVLVRLLRERLESLLPRHLGGLAELSGRVRHRAKELLSSISDRRRFWERAFASNALAGLIEKQDWQRAEAWLNEIAGTRVGEVFVVGAGPGDPELLTIKALQAMQTADVIVYDYLVSDEIMALVRKDAELVCVGKRLGNHSVPQDEINQILVELAKQGKKVCRLKGGDPFIYGRGGEEVQELAKHQIAFQIVPGITAAAGCSAYAGIPLTHRDHAQAIQFVTGHCKKEGQELDWQSLAKANQTLAVYMGVIKSPHIQAKLIEYGRAPETPVAIIENGTRKEQRVVTGELNDLANLIEREQIKSPALLIIGEVANLGEVYLVGAGPGDPDLLTFRALRLMQQADVVLYDRLVAPAILDLCRRDADRIYVGKQRAAHAVPQDQINQLLVTLAKEGKRVLRLKGGDPFIFGRGGEEIEELAAHDVPFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFVTGHLKDGSCDLPWSELVAPSQTLVFYMGLVGLPVICQQLIAHGRAADTPAALVQQGTTINQRVFTGTLANLAERVANEQVQAPTLVIIGEVVQLHGQLAWF"
  },
  {
    "reference": "MYSINRIGIHGISNKILSRPKFFHSSSSIFSLNQNLNNFKNNNNYILNGYNKITHNHHHQTLFHRHYSNLKQEQENKQQNVGKNSETSPSSTTTTENITTATTATTKTDIKETQNSTMSMLKTVFKYLWPKDNNDSKIRIITSVLLLLSAKVLTVQIPFIFKDIVDSLTTTQSEMLTLPLGLLLAYGAVKITSNGFQELRQTIFSKVAHDAIRDVSCSTFKRLHQLDLTFHLSRQTGSLSRIIDRGGRGINFLLNSILFHVVPTAFEISLVSYVMYTTLGWEYSALSLATIAAYTVFTVKVTKWRTQFRVKMNKMDNEASNKMMDSLINFETVKYFNNDALEVERYHNYLKEYDKASLKTTSSLSFLNFGQALIFSLSMTAMMIMAAQGVVQGNLSVGDLVLVNGLLFQISLPLNFLGTVYREIKQSLVDMDHLFSLLNLNPKISDNKDSKPLKLENGTIVFRDISFKYNDSVQVLNNVSFECEGGKRIAIVGSSGSGKSTLLRLLYRFYDVSSGSIEIDGQDIRGIQLESLRKHIGVVPQDTVLFNDTIYYNIAYGNPNATKEQVENAARAAHIHEVILNMKNGYDTVVGERGLKLSGGEKQRVSIARAILKDSPIVFYDEATSSLDTEKEKLIMDALRELFKGRTTIMIAHRLSTIVDADEIIVLGTGGIILERGNHQQLLELEGKYRSMWLAQQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein can be targeted to mitochondrial inner membrane, ATP-binding cassette (ABC) transporter complex and perform ATP hydrolysis activity, ATPase-coupled transmembrane transporter activity, ATP binding.2. The protein should contain a Helical membrane-spanning region.3. The designed protein must have at least one ABC transmembrane type-1 domain. The designed protein sequence is ",
    "response#1": "PGSTVAIVGPTGAGKTTLVNLLMRFYEIGSGRILIDGVDIASVSRQSLRSRIGMVLQDTWLYDGTIAENIAYGRPEATTDEIVEAARAAHVDRFVNTLPAGYQTRVSGDGGSISVGEKQLITIARAFLARPQLLILDEATSSVDTRTELLIQRAMRELRRDRTSFIIAHRLSH",
    "response#2": "PGRVTAIMGPSGAGKTTFLNAVLGKTTGYKKDGLVLINGKSGSMQSYKKIIGFVPQDDIVHGNLTVEENLWFSACCRSSKGMSKSDKIIVLERVIGSLGLQEIRNSLVGTVEKRGISGGQRKRVNVGIEMVMEPSLLILDEPTTGLDSASSQLLLRALRHEALQGVNVCAVIHQPSYTLFNMFDDFVLLARGGLIAYLGPIT",
    "response#3": "CVTGKLSPGRVTAIMGPSGAGKTTFLNAVLGKTTGYKKDGLVLINGKSGSMQSYKKIIGFVPQDDIVHGNLTVEENLWFSACCRSSKGMSKSDKIIVLERVIGSLGLQEIRNSLVGTVEKRGISGGQRKRVNVGIEMVMEPSLLILDEPTC"
  },
  {
    "reference": "RWDVDQCIYYCLNGVVGYSYTECQTMCT",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: When intracranially injected, elicits scratching and grooming activity in mice, and causes body and rear limb extension and tail curling immediately upon injection..2. The protein must be able to toxin activity. The designed protein sequence is ",
    "response#1": "DQENGGKALASPPGNWQKADVTFDSNTAFESLVVSPDKKTVENVGVPKGVPDSPERFSSSPCVLGSPGFRSGKHFFEVKYGTQREWAVGLAGKSVKRKGYLRLVPEERIWQKGLWWLRRLETDSDKLQKGSGKIIVFLDYDEGKVIFDLDGEVTTIQANFNGEEVVPFYYIGARVSLANC",
    "response#2": "DQENGGKALASPPGNWQKADVTFDSNTAFESLVVSPDKKTVENVGVPKGVPDSPERFSSSPCVLGSPGFRSGKHFFEVKYGTQREWAVGLAGKSVKRKGYLRLVPEERIWQKGLWWLRRLETDSDKLQKGSGKIIVFLDYDEGKVIFDLDGEVTTIQANFNGEEVVPFYYIGARVSLANC",
    "response#3": "DQENGGKALASPPGNWQKADVTFDSNTAFESLVVSPDKKTVENVGVPKGVPDSPERFSSSPCVLGSPGFRSGKHFFEVKYGTQREWAVGLAGKSVKRKGYLRLVPEERIWQKGLWWLRRLETDSDKLQKGSGKIIVFLDYDEGKVIFDLDGEVTTIQANFNGEEVVPFYYIGARVSLANS"
  },
  {
    "reference": "MTGQTHFGFRQVAPEEKRRRVMALFSSVAERYDLMNDLMSLGLHRWWKRCMVASAAVRRGGRVLDVAAGSGDIALALADRVGPEGRVVLLDPNRPMLAEGAARLIDAGLLPGRADCIQGDGAALPFADRSFDQVTIAFGLRNFVDPERGLAEFQRVLRPGGQLLVLEFSRPVLPLLEPLYDAWSFHLIPLLGEKVAGDRAAYQYLVESIRRFPDQRRLCRWMEQAGFALVRYRNLSGGIVAIHRGYRL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should interact with enzymes and substrates within the menaquinone biosynthesis pathway to enhance or regulate its activity.2. The protein should be able to bind S-adenosyl-L-methionine ligand in a variety of conditions3. The designed protein must be able to regulate aerobic respiration. The designed protein sequence is ",
    "response#1": "MPIGVPKVPFRSPGEEDASWVDIYRK",
    "response#2": "MLLKNTRILA",
    "response#3": "MVDPFGRQL"
  },
  {
    "reference": "MKKKIGLLVMAYGTPYKEEDIERYYTHIRRGRKPSPEMLEDLTERYRAIGGISPLATITLEQAKKLEKRLNEVQDEVEYHMYLGLKHIEPFIEDAVKDMHNDGIQDAIALVLAPHYSTFSVKSYVGRAQEEAEKLGNLTIHGIDSWYKEPKFIQYWVDAVKGIYNGMSDAEREKAVLIVSAHSLPEKIIAMGDPYPEQLHETADYIARGAEVANYAVGWQSAGNTPDPWIGPDVQDLTRELNEKHGYTSFVYAPVGFVAEHLEVLYDNDFECKVVTDEIGAKYYRPEMPNASDAFIDCLTDVVLKKKESVL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Catalyzes the insertion of ferrous iron into coproporphyrin III to form Fe-coproporphyrin III.. The designed protein sequence is ",
    "response#1": "FIEGIQDCLEQVKERGFKVSRTAILFSTHSIPELDAAFSGPEDAHFGKYGAYVSQHKAVVEVIMSRLRVTDPDLQNYQLVYQSRSGDPSTPWLEPDINDAIRVLRGCEAVLIVPLGFISDHMEVLWDLDNESMQTARECGLFAIRTPTPS",
    "response#2": "FIEGIQDCLEQVKERGFKVSRTAILFSTHSIPELDAAFSGPEDAHFGKYGAYVSQHKAVVEVIMSRLRVTDPDLQNYQLVYQSRSGDPSTPWLEPDINDAIRVLRGCEAVLIVPLGFISDHMEVLWDLDNESMQTARECGLFAIRTPTPR",
    "response#3": "FIEGIQDCLEQVKERGFKVSRTAILFSTHSIPELDAAFSGPEDAHFGKYGAYVSQHKAVVEVIMSRLRVTDPDLQNYQLVYQSRSGDPSTPWLEPDINDAIRVLRGCEAVLIVPLGFISDHMEVLWDLDNESMQTARECGLFAIRTPTPW"
  },
  {
    "reference": "MSTQNSAYSAFSSLLAEAAMPVSPAELHGHLLGRVCAGAGFDEAAWQHAAAELLGGAPGERLKAALSGLLGMVRQDFSAGEVAVVMLLPDDETPLAQRTEALGQWCQGFLAGFGLTAREGSLTGEAEEVLQDMAAIAQVQGQLEDSEDGETDYMEVMEYLRVAPLLLFAECGKPLEPAPKPSLH",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be expressed specifically in the cytosol The designed protein sequence is ",
    "response#1": "LLKLQLVKSGSQMRTMLRKETSISTRFYKYFVEQMHPTMVYLTKALRKSVLNVIQLGNFWLDVDMKQSSSRFLSDGRQNSERLPEYRALVVSKLVDFVDNFLENISLALSMLPPNLNWIVRDIFCSLYEIVDDMSAIELSHACKDMIVSNLLCPAIISPQKFGVVDNDVRIGSIVNHNLAQIAMIIQMV",
    "response#2": "LLKLQLVKSGSQMRTMLRKETSISTRFYKYFVEQMHPTMVYLTKALRKSVLNVIQLGNFWLDVDMKQSSSRFLSDGRQNSERLPEYRALVVSKLVDFVDNFLENISLALSMLPPNLNWIVRDIFCSLYEIVDDMSAIELSHACKDMIVSNLLCPAIISPQKFGVVDNDVRIGSIVNHNLAQIAMIIQMV",
    "response#3": "LLKLQLVKSGSQMRTMLRKETSISTRFYKYFVEQMHPTMVYLTKALRKSVLNVIQLGNFWLDVDMKQSSSRFLSDGRQNSERLPEYRALVVSKLVDFVDNFLENISLALSMLPPNLNWIVRDIFCSLYEIVDDMSAIELSHACKDMIVSNLLCPAIISPQKFGVVDNDVRIGSIVNHNLAQIAMIIQMI"
  },
  {
    "reference": "MNDMAKNLILWMIIAGVLLTVFNNINQEPREDNINYSEFVRDVRSGRVSAVELAGINISGIRTDNTQFRSIMPLIGDDQLMGDLFENGVEIIANEPEQQSIWSQLLVASFPILIIIALFFFFMRQMQGGGPGGRGGPMSFGKSKAKLLGEDQIKVTFADVAGVDEAKEDVQELVEFLREPDKFQKLGGRIPRGILMVGQPGTGKTLLAKAIAGEAKVPFFSISGSDFVEMFVGVGASRVRDMFDQAKKQAPCIVFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFEANDGVIVVAATNRPDVLDPALLRPGRFDRQVVVSLPDIRGREQILKVHMRKIPIDDRVKASVIARGTPGFSGADLANLVNEAALFAARAGRRLVTMEEFEKAKDKIMMGAERKSMVMSEKERLNTAYHESGHAIVGRLMPDHDPVYKVSIIPRGRALGVTMFLPEEDRYSYSRQHILSQICSLYGGRIAEEMTLGKEGVTTGASNDIQRATEIARNMVTKWGLSEKLGPVLYSEDEGEVFLGRSAASQPKSVSNDTAKTIDSEVRKIVDECYTRAREILDNNQNLMELMKDALMEYETIDSEQIDDIMDGKKPRPPADWTDSDNDDSGVSNVEKEKVGDSRKIGGPASEH",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that localizes to plasma membrane and can perform metalloendopeptidase activity with high specificity and efficiency.2. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "VLLVGPPGTGKTLLAKAVAGEAGVPFFSLSGSDFVELFVGVGAARVRDMFAQAVQKAPSIIFIDELDALGKRRGDGGPGNHDERDQTLNALLVEMDGFGTDQSVIVMGATNRPEILDPALMRPGRFDRHVLVL",
    "response#2": "VMLSGPPGTGKTLLAKAVAGEAKVPFFSLSGSDFVEMFVGVGASRVRDLFKQAKEKSPCIIFIDEIDAIGRARGKNNFSGGNDERENTLNQLLTEMDGFGTNEHVIVMAATNRADILDRALMRAGRFDRIIYVSFF",
    "response#3": "IPRGVLLVGPPGTGKTLLAKAVAGEAGVPFFSLSGSDFVELFVGVGAARVRDMFAQAVQKAPSIIFIDELDALGKRRGDGGPGNHDERDQTLNALLVEMDGFGTDQSVIVMGATNRPEILDPALMRPGRFDRHVLVDKPD"
  },
  {
    "reference": "MPPFLLLTCLFITGTSVSPVALDPCSAYISLNEPWRNTDHQLDESQGPPLCDNHVNGEWYHFTGMAGDAMPTFCIPENHCGTHAPVWLNGSHPLEGDGIVQRQACASFNGNCCLWNTTVEVKACPGGYYVYRLTKPSVCFHVYCGHFYDICDEDCHGSCSDTSECTCAPGTVLGPDRQTCFDENECEQNNGGCSEICVNLKNSYRCECGVGRVLRSDGKTCEDVEGCHNNNGGCSHSCLGSEKGYQCECPRGLVLSEDNHTCQVPVLCKSNAIEVNIPRELVGGLELFLTNTSCRGVSNGTHVNILFSLKTCGTVVDVVNDKIVASNLVTGLPKQTPGSSGDFIIRTSKLLIPVTCEFPRLYTISEGYVPNLRNSPLEIMSRNHGIFPFTLEIFKDNEFEEPYREALPTLKLRDSLYFGIEPVVHVSGLESLVESCFATPTSKIDEVLKYYLIRDGCVSDDSVKQYTSRDHLAKHFQVPVFKFVGKDHKEVFLHCRVLVCGVLDERSRCAQGCHRRMRRGAGGEDSAGLQGQTLTGGPIRIDWED",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a specific function in urate homeostasis and should be localized to cell surface, extracellular space, nuclear envelope in order to carry out this function efficiently.2. The presence of ZP domains is critical for the interaction of the designed protein with its intended targets.3. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "NGQYCESGEAFCFEGRCQTADKQCMSMLGKYVRGASFACYEEFNSRGDRFGNCIHNFCAFRNSLCGKLICTWPFKKLVLKANLSVAYAQIRDDLCVAMC",
    "response#2": "IPPTLDWRKEGYVTPVRRQGSCGACWAFSVTACIEGQLFKKTGKLIPLSVQNLMDCSVSYGTKGCDGGRPYDAFQYVKNNGGLEAEATYPYEAKAKHCRYRPERSVVKVNRFFVVPRNEEALLQALVTHGPIAVAIDGSHASFHSYRGGIYHEPKCRKDTLDHGLLLVGYGYEGHESENRKYWLLKNSHGERWGENGYMKLPRGQNNYCGIASYAMYPL",
    "response#3": "IPPTLDWRKEGYVTPVRRQGSCGACWAFSVTACIEGQLFKKTGKLIPLSVQNLMDCSVSYGTKGCDGGRPYDAFQYVKNNGGLEAEATYPYEAKAKHCRYRPERSVVKVNRFFVVPRNEEALLQALVTHGPIAVAIDGSHASFHSYRGGIYHEPKCRKDTLDHGLLLVGYGYEGHESENRKYWLLKNSHGERWGENGYMKLPRGQNNYCGIASYAMYAR"
  },
  {
    "reference": "MAPRKPKVVSVTKKKKVVEETIKVTVTEEGDPCVITETANDQETQDLTFSIPVGENVTTVEIPVEVPDERSLPVGENVTTVKIPVDDRDESSPQPPETPVEVRDEPSPQPPETPASKSEGTLKKTDKVEKKQENKKKKKKKKRDDLAGDEYRRYVYKVMKQVHPDLGITSKAMTVVNMFMGDMFERIAQEAARLSDYTKRRTLSSREIEAAVRLVLPGELSRHAVAEGSKAVSNFVGYDSRKR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should exhibit Pro residues compositional bias.2. The protein can be expressed in nucleus and perform protein heterodimerization activity, structural constituent of chromatin without triggering undesirable cellular responses.3. For general function, the protein need meet that Core component of nucleosome The designed protein sequence is ",
    "response#1": "AGLQFPHPPVSEMVFNAVKTLKERGGSSLQAIKKFLIAQYKVDVDKLDTFIKKYLKSAVEKGQLLQTKGKGALGSFKC",
    "response#2": "MELPIAPIGRIIKNAGAEIVSDDAREALAKVLEAKGEEIAENAVKLAKHAGRKTVKASDIELAN",
    "response#3": "PPTDGVKEPHRYRPGKMGLREIRKYKKNARFFISKLPFHRLVRKITQNLKAHLRFQSTAMPAPEEVSEAYLVKLFEDAIHAKRVTLVPKDIQLARCR"
  },
  {
    "reference": "MRVMGTLRNYQQWWIWGVLGFWMLMCCNMDKNLWVTVYYGVPVWRDAKTTLFCASDAKAYETEVHNVWATHACVPTDPNPQEILMENVTEPFNMWKNDMVDQMHEDVISLWDQSLKPCVKLTPLCVTLRCVNVSSDNSTHDNSTYNDSGMKNCSFNTTTVLRDKQKAEYALFYRLDIVPLDDSSNNSSEKSSEYRLINCNTSAITQACPKVTFDPIPIHYCTPAGYVILKCNDKMFNGTGPCRNVSSVQCTHGIKPVVSTQLLLNGSLAEEGIIIKSKNLTDNVKTIIVHLNESVEITCTRPNNNTRKSIRIGPGQTFYATGGIIGDIRQAYCNISKSEWGKALYNVSKKLAEHFPNKTIKFNSSSGGDLEIITHSFNCRGEFFYCNTSGLFNSTYMPDTDTYMFNDTYMLNGTYMFNNTRDNSPTITIPCRIKQFINMWQEVGRAMYAPPIAGNITCKSKITGLLLVRDGGKTNKTDDIEIFRPGGGDMRDNWRNELYKYKVVEIKPLGLAPTAAKRKVVEREKRAVGLGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKTQEEIWDNMTWLQWDKEINNYTTTIYRLLEESQNQQENNEKDLLALDSWKNLWSWFDISKWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQIRTPNPGGPDRLGRIEEEGGEQDKSRSIRLVSGFL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be involved in clathrin-dependent endocytosis of virus by host cell, actin filament reorganization, positive regulation of establishment of T cell polarity by localizing to virion membrane, host cell endosome membrane, host cell plasma membrane, plasma membrane, viral envelope and exhibiting structural molecule activity.2. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.3. The protein contains novel Retroviral envelope protein GP41-like, Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains that confer a unique function or activity.4. The protein must contain a signal peptide for proper functionality. The designed protein sequence is ",
    "response#1": "WVTVYYGVPVWRDAETTLFCASDAKAHDKEVHNVWATHACVPTDPNPQEMILENVTEDFNMWKNSMVEQMHTDIISLWDQSLKPCVKLTPLCVTLNCSDSNITSNSTSNSTKDSATLDMKSEIQNCSFNMTTELRDKKQKVYSLFYRLDVVQINENSSDYRLINCNTSAITQACPKVTFEPIPIHYCAPAGFAILKCNDKKFNGTGPCTNVSTVQCTHGIKPVVTTQLLLNGSLAEEEVMIRSENITENAKNIIVQFKEPVQIICIRPGNNTRKSVHIGPGQAFYATGDIIGDIRQAHCNVSRELWNKTLQEVATQLRKHFRNNTKIIFTNSSGGDVEITTHSFNCGGEFFYCDTSGLFNSSWTASNDSMQEAHSTESNITLQCRIKQIINMWQRAGQAMYAPPIPGIIRCESNITGLILTRDGGEGNNSTNETFRPVGGNMRDNWRSELYKYKVVKVEPLGVAPTKSRRRVVEREKR",
    "response#2": "MGAAAVTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLRDQQLLGIWGCSGKLICTTAVPWNSSRSNKSEDEVWNMTWMQWEREIDNYTDIIYKLLGDSQNQQEQNEKDLLALDKWDSLWSWFNITNWLWYIKIFIMIVGGLIGLRIILGVLSIVNRVRQGYSPLSFQTGR",
    "response#3": "LWVTVYYGVPVWKEATTTLFCASDAKAYETEVHNIWATHACVPTDPSPREVVLENVTENFNMWKNDMVEQMHEDIISLWDESLKPCVKLTPLCVTLHCTNLENTTNTSSNTSSMTEGGEMKNCSFNITTSIKTKVQDYALFYKPDIVQIDNSNTSYRLIHCNISVITQACPKVSFEPIPIHYCTPAGFTILKCNNKKFKGAGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSSNFSDNAKVIIVQLNESVEINCTRPNNNTRKRVSIGPGRAWYTTKQIVGDIRQAHCNISRTKWNDTLKQITEKLREQFGNKTIIFNQSSGGDPEIVRHSFNCGGEFFYCNTAQLFNSTWNNTSTWNGTTGNSIITLPCRIRQIVNMWQKVGKAMYAPPIEGPIKCSSNITGLLLTRDGGHNESNTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR"
  },
  {
    "reference": "MAFQDFDKIQERVNANRKRKFRKRIIVGTVSLLVVVAAIVGGAFAYVAYEKRNEQQQQQQQAKNHNKSGSGNNVVKDSDKKSPSPPTPSQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQETCLDGFEEGNLKSEVKTSVNSSQVLTSNSLALIKTFTENLSPVMKVVERHLLDDIPSWVSNDDRRMLRAVDVKALKPNATVAKDGSGDFTTINDALRAMPEKYEGRYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVKEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGDTTSRVKWPGFKVINKEEALNYTVGPFLQGDWISASGSPVKLGLYDA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Acts in the modification of cell walls via demethylesterification of cell wall pectin..2. The substrate binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein should be optimized for pectinesterase activity, aspartyl esterase activity activity in order to improve pectin catabolic process.4. The protein with the reaction [(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O =   [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol, the Proton donor; for pectinesterase activity must be optimized to ensure efficient catalysis.5. A protein should be able to manipulate the activity of enzymes in the pectin degradation, Glycan metabolism, 2-dehydro-3-deoxy-D- gluconate from pectin: step 1/5 pathway, either by activating or inhibiting their function.6. The designed protein's functionality will benefit from Polar residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "DMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGVNIHDCKRKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGSTAITISNNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWQMYAIGGSAAPTINSQGNRFLAPNDNVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRFTPARFLRGDIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGVYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFINTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSASVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKNFSTTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLSTKEANRFTVKPFID",
    "response#2": "DMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGVNIHDCKRKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGSTAITISNNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWQMYAIGGSAAPTINSQGNRFLAPNDDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRIREGVYEETVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMARDLTIENTAGADAHQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFIFGNSAAVFQDCDILIASKHSKLEQGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMKEFQANPEGHKNFLGRPWKEFSRTVFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTGPGSVRSSRVPWSSEIPEKHVDVYSVANFIQADVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRIREGVYEETVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMARDLTIENTAGADAHQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFIFGNSAAVFQDCDILIASKHSKLEQGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMKEFQANPEGHKNFLGRPWKEFSRTVFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTGPGSVRSSRVPWSSEIPEKHVDVYSVANFIQA",
    "response#3": "DMTIQLKEELIMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGLHIHDCKQGGNTYVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGSTAITISNNYLTHHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPDDDIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGVYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFINTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSASVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKNFSTTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLSTKEANRFTVKPFIDDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRIREGVYEETVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMARDLTIENTAGADAHQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFIFGNSAAVFQDCDILIASKHSKLEQGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMKEFQANPEGHKNFLGRPWKEFSRTVFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTGPGSVRSSRVPWSSEIPEKHVDVYSVANFIQA"
  },
  {
    "reference": "MPASSPFLLAPKGPPGNMGGPVREPALSVALWLSWGAALGAVACAMALLTQQTELQSLRREVSRLQGTGGPSQNGEGYPWQSLPEQSSDALEAWENGERSRKRRAVLTQKQKKQHSVLHLVPINATSKDDSDVTEVMWQPALRRGRGLQAQGYGVRIQDAGVYLLYSQVLFQDVTFTMGQVVSREGQGRQETLFRCIRSMPSHPDRAYNSCYSAGVFHLHQGDILSVIIPRARAKLNLSPHGTFLGFVKL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be optimized for stability and solubility when expressed in extracellular space, extracellular exosome, extracellular region where it will be involved in positive regulation of isotype switching to IgA isotypes, signal transduction. The designed protein sequence is ",
    "response#1": "EVLTQTPSPVSAAVGGTVTISCQSTKSIYBBBYLAWYQZKPGQPPKALIYTASSLASGVPSRFTGSGSGTZFTLTLSDVZCDDAATYYS",
    "response#2": "VQPGRSLRLSCAASGFTFDDYAMHWVRQAPGKGLEWVSGISWNSGSIGYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTALYYCR",
    "response#3": "PLLLAVLAHTSGSLVQAALTQPSSVSANPGETVKITCSGDRSYYGWYQQKAPGSAPVTLIYDNTNRPSNIPSRFSGSKSGSTATLTITGVQADDEAVYYCGSADSSSTC"
  },
  {
    "reference": "MGDSERRKSERRRSLGYPSAYDDVSIPARRPSTRTQRNLNQDDLSKHGPFTDHPTQKHKSAKAVSEDVSSTTRGGFTNKPRAKPGVRAVQSNKFAFSTAPSSASSTWRSNTVAFNQRMFCGAVATVAQYHAYQGALALWRQDPPRTNEELDAFLSRAVIKITIQEGPNLMGEAETCARKLLEESGLSQGNENVKSKSERTTKSERTRRGGEIEIKSPDPGSHRTHNPRTPATSRRHHSSARGYRSSDSE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein localizes to the host cell Golgi apparatus.2. For general function, the protein need meet that Tegument protein that plays different roles during the time course of infection ; Acts by mediating disruption of liquid-like droplets in which CGAS is activated, thereby preventing CGAS activity .; Plays a role in the inhibition of host innate immune system by targeting the CGAS enzymatic activity which is the principal cytosolic DNA sensor that detects invading viral DNA; Plays a role in microtubule reorganization that occurs after viral infection by stabilizing microtubule network 3. The designed protein's functionality will benefit from Basic and acidic residues, Polar residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "ALLKTSPSPERALFLKYARTPTLDPDTMHARLRLSPDGLTVRCSLLGRLGPRPAPRFDALRQVLGRDGFAAGRHYWEVDVQEAGVGWWVGAAYPSLRRRGASAAARLGCNRESWCVKRYDLEYWAFHDGQRSRLRPRRDPHRLGVFLDYEAGILAFYDVAGGMSHLHTFHAAFQEPLYPALRLWEGPISIPRLR",
    "response#2": "ALLKTSPSPERALFLKYARTPTLDPDTMHARLRLSPDGLTVRCSLLGRLGPRPAPRFDALRQVLGRDGFAAGRHYWEVDVQEAGVGWWVGAAYPSLRRRGASAAARLGCNRESWCVKRYDLEYWAFHDGQRSRLRPRRDPHRLGVFLDYEAGILAFYDVAGGMSHLHTFHAAFQEPLYPALRLWEGPISIPRLY",
    "response#3": "ALLKTSPSPERALFLKYARTPTLDPDTMHARLRLSPDGLTVRCSLLGRLGPRPAPRFDALRQVLGRDGFAAGRHYWEVDVQEAGVGWWVGAAYPSLRRRGASAAARLGCNRESWCVKRYDLEYWAFHDGQRSRLRPRRDPHRLGVFLDYEAGILAFYDVAGGMSHLHTFHAAFQEPLYPALRLWEGPISIPRLR"
  },
  {
    "reference": "MSRTFDVAVVGATGAVGQAMMEILAQRGFPVSRVYPLASERSAGEKLSFGRDEVIVENLAGFDFSKVQLGLFSAGAKISAEYAPKAASAGCVVVDNTSQFRYDNRIPLVVPEVNPQAIEGYKDHGIIANPNCSTIQMLVALKPIYDAVGIERINVATYQAVSGTGKEAIEELAKQTSTLLNGRPISPQAYPKQIAFNVLPHIDDFLDNGYTREEMKMVWETRKIFGDESILVNPTAVRVPVFYGHSEAVHLETRDKITADEVKALLQQAPGVTVLDEHTNGGYPTAVTEASGRDPVFVGRIREDISHPKGIDLWIVADNVRKGAALNSIQIAELLIKDYL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must possess NADP binding, aspartate-semialdehyde dehydrogenase activity, NAD binding, protein dimerization activity to support threonine biosynthetic process, isoleucine biosynthetic process, diaminopimelate biosynthetic process, 'de novo' L-methionine biosynthetic process under certain conditions.2. A protein that is capable of catalyzing reactions within the L-threonine biosynthesis, L-threonine from L-aspartate: step 2/5, Amino-acid biosynthesis pathway with high efficiency and specificity.3. The designed protein should be able to compete with endogenous proteins or inhibitors for NADP(+), phosphate, substrate binding.4. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.5. The designed protein have Semialdehyde dehydrogenase NAD-binding domains with specific sequence motifs that are crucial for its activity.6. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate. The designed protein sequence is ",
    "response#1": "HVGVVGATGQVGVAMRQILAERGFPAASVRFFASARSAGTRLPWGDGEVVVEDAATADPAGLDVALFSAGATTSRAQAPRFAAAGVVVIDNSSAFRKDPDIPLVCSEVNPDDVDLDQGIIANPNCTTMAAMPVLKPLHAEAGLTRIVASTYQAVSGSGVAGVRELHGQVQAVVEKADALAYDGRALQFPAPEKYVAPIAFNVLPMAGSVVDDGSFETDEEQKLRNESRKILHIPDLRVSGTCVRVPVFTGHSLSLNVEFARPLSVERARELLAGAPGVELVDVPTPLQAAGQDPSYVGRIRQDGPTGLALFVSNDNLRKGAALNAIQVAELLL",
    "response#2": "MSKIAIVGASGAVGREFLRLLEHRDFPIDELRLFGSSRSAGSEITFRGKEYKIKLLAQNDDFKDIDIAFVSAGGQTALDFASTITKHGALMIDNSSAFRMDPEVPLVVPEVNGCTTNCLAPIHALSPIERVHVATYQAASGAGHDAMLELEGQVRDQAMGKPLQTKAFGNQLLYNLIPQIDIFTDNDYTKEEMKMYNETCKILHNDEIRVSATCVRVPVLRAHSEAIWVKCAEPLTVEAVREAMSKQKGLLLMDDPAKRSYPMPLHCSMQEPVYVGRLRADLAEPGCVTFWCVADQIMYDNEWGYSNRLIDLVLHMAKL",
    "response#3": "MTKVAIVGASGAVGVEMLRVMERRNFPVASLRLLASPRSAGKTLTFRGEKIKVEPLHLNSFEGIDIALFSAGGAISRDFAHAAVTSGAVVVDNSSAFRMSQGVPLVVPEINGADVKLGGLFERDLVEWVSVATYQAASGAGAKNMRELLVQMGQLHNVVEADLANPASSILDIERAVTAKMRADLPCDNFGAPLAGSLIPWIDKLLESGQTKEEWKGYAETNKILGLSENPVPIDGLCVRIGALRCHSQAFTIKMKKDLPLAEIEQILASHNEWVKVIPNDKETTLRELTPAKVTGTLSVPVGRLRKLNMGPEYLAAFTVGDQLLAEGKTRNIIANPNCSTMQMVVALAPIHALSPIERVHVATYQAASGAGHDAMLELEGQVRDQAMGKPLQTKAFGNQLLYNLIPQIDIFTDNDYTKEEMKMYNETCKILHNDEIRVSATCVRVPVLRAHSEAIWVKCAEPLTVEAVREAMSKQKGLLLMDDPAKRSYPMPLHCSMQEPVYVGRLRADLAEPGCVTFWCVADQIMKGAATNTVQIAEILVADGLVK"
  },
  {
    "reference": "MADGRPAALDDFCRRFDISFFDLHLTCIFCSHTVDLQDLASFYLKKLSLVFRGGCYYACCSECLRLSARFEQENYFQCSIKAVNLEEVAQRKIKEICIRCICCLRLLDIVEKLDLLYSDQACYLIRGLWRGYCRNCIRKQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be involved in regulation of DNA-templated transcription, DNA-templated transcription, suppression by virus of host type I interferon-mediated signaling pathway, modulation by virus of host apoptotic process by localizing to host cell cytoplasm, host cell nucleus and exhibiting metal ion binding.2. The protein must exhibit the following characteristics: E6 associates with host UBE3A/E6-AP ubiquitin- protein ligase and modulates its activity; May also inhibit host immune response.; Protects host keratinocytes from apoptosis by mediating the degradation of host BAK1. The designed protein sequence is ",
    "response#1": "XMMXGXRXL",
    "response#2": "LWGN",
    "response#3": "MEFGLS"
  },
  {
    "reference": "MKMIIQIFFIISLISTISFASVQDFCVADPKGPQSPSGYSCKNPDQVTENDFAFTGLGTAGNTSNIIKAAVTPAFAPAYAGINGLGVSLARLDLAGGGVIPLHTHPGASEVLVVIQGTICAGFISSANKVYLKTLNRGDSMVFPQGLLHFQLNSGKGPALAFVAFGSSSPGLQILPFALFANDLPSELVEATTFLSDAEVKKLKGVLGGTN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Probably has no oxalate oxidase activity even if the active site is conserved.; May play a role in plant defense.2. The protein should be optimized for extracellular matrix, nucleus, apoplast, plastid-specific folding and stability, while maintaining manganese ion binding.3. Design the protein with a signal peptide incorporated.4. The designed protein must have at least one Cupin type-1 domain. The designed protein sequence is ",
    "response#1": "FDMPRNTFNKLGSNVTNLNVMEFPGLNTLGISLARIDYAPMGVNPPHIHPRATELLTVLEGTLYVGFVTSNPNKLFSKVVCKGDVFVFPKAMIHFQMNLDHDKPAVAQSALSSQNPGVITIASAVFGSQPPISDDVLTKAFQVEKC",
    "response#2": "SLNRPGNTNNAVGSNVTTVNVNNLGGLNTLGISLVRIDYAPNGQNPPHTHPRATEILVVQQGTLLVGFISSNQDGNRLFAKTLNVGDVFVFPEGLIHFQFNLGGTPAVAIAALSSQNAGVITIANTIFGSKPDVDPNVLARAFQMDVNAV",
    "response#3": "FNAAKFDMPRNTFNKLGSNVTNLNVMEFPGLNTLGISLARIDYAPMGVNPPHIHPRATELLTVLEGTLYVGFVTSNPNKLFSKVVCKGDVFVFPKAMIHFQMNLDHDKPAVAQSALSSQNPGVITIASAVFGSQPPISDDVLTKAFQVEKKLIG"
  },
  {
    "reference": "MKVLAAMSGGVDSSVAAARMVDAGHDVVGVHLALSTVSGTLRTGSRGCCSKEDVSDARRVADVLGIPFYVWDFAEKFQEDVIDNFVSAYARGETPNPCVQCNQRIKFSALSVRALMLGFDTVATGHYARLSGGRLRRAVDRGKDQSYVLAVLTAQQLRHAAFPIGDTLKRQVRAEAARRGLLVADKPDSHGICFIPSGNTRAFLSECIGIRRGSVVDADGTVLAEHDGVHGFTVGQRKGLGIVGPGPNGRPRYVTAIDADTATVYVGDAADLGVHTLIGRAPVFTAGAAPLGPVQCVVQIRAHGETAVAVVELIGDELFVRLCEPLRGVARGQTLVLYRLDPDGDEVLGSATIASMSA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that can perform ATP binding, sulfurtransferase activity, tRNA binding in cytoplasm to support tRNA modification.2. For general function, the protein need meet that Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34.3. The Nucleophile, Cysteine persulfide intermediate of the protein must be engineered to enable effective catalysis of AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in   tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-   cysteinyl-[protein].4. The protein should be able to bind ATP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "FEAEVLHTVLAGHLLPPGAVVAVGASGGKDSTVLAHVLRELAPRLGITLHLVAVDEGIGGYRDAALEAVRSQAARWELPLTIVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARLVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGVLGSTGEGCALPRCRPLQFASQKEVVLYAHFRHLRYFSEECVYAPERHDWAFCDKFSVIDFNLLVDIDKNFEATVKVRKNSTEVPCKVSLENHRVIVKTTMPVFAVAPGQAAVFYRGDIVIGAGIA",
    "response#2": "MWLKDWNIVDESRLLGCDDVIVKIRYRKQENHCSVTITPEGLLHIRLHEPLSAIAEGQAAAFYKDGLLLGGGID",
    "response#3": "VAVGASGGKDSTVLAHVLRELAPRLGITLHLVAVDEGIGGYRDAALEAVRSQAARWELPLTIVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARLVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGVLGSTGEGCALPRCRPLQFASQKEVVLYAHFRHLRYFSEEDAAYCGTFEAEVLHTVLAGHLLPPGAVVAVGASGGKDSTVLAHVLRELAPRLGITLHLVAVDEGIGGYRDAALEAVRSQAARWELPLTIVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARLVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGVLGSTGEGCALPRCRPLQFASQKEVVLYAHFRHLRYFSEECVYAPEGGQYACSVCGFEAEVLHTVLAGHLLPPGAVVAVGASGGKDSTVLAHVLRELAPRLGITLHLVAVDEGIGGYRDAALEAVRSQAARWELPLTIVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARLVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGVLGSTGEGCALPRCRPLQFASQKEVVLYAHFRHLRYFSEECVYAPEGGQYACSVCGFEAEVLHTVLAGHLLPPGAVVAVGASGGKDSTVLAHVLRELAPRLGITLHLVAVDEGIGGYRDAALEAVRSQAARWELPLTIVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARLVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGVLGSTGEGCALPRCRPLQFASQKEVVLYAHFRHLRYFSEECVYAPE"
  },
  {
    "reference": "MCSAPNGRKNRPRSAANIFQIGKSSVRDPERRESTESARRAQRAMDDCRTTVQEFNTLVALHREQVISIGENTTDCPSLRAQMHKTRVKGCAVAQAAYQNLIAISGPEDGEIHPEICRLFIQLQCCLEMYITEMLKSMCLLGVLQLHRKGNDMCPELNMDCRVDESSDVPMLEDRSSSPMDFPQDSWVVCADIENIESDMREMRNLLSKLRETMPLPLKNQDDSSLLNLTPYPLVRQRKRRFSGLCCLVSG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein localizes to the neuron projection.2. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "EDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQSGTEPINTFHGIHQNNDEPIRVSYHRNIHR",
    "response#2": "EDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQSGTEPINTFHGIHQNNDEPIRVSYHRNIHF",
    "response#3": "FVIKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQSGTEPINTFHGIHQNNDEPIRVSYHRNIHYNSVVNP"
  },
  {
    "reference": "MSLLTEVETXTXTGWECNCSGSSDPXXXXXXXXGILHLILWILDRLFFKCIYRRFKYGLKRGPSTEGMPESMREEYRQEQQNAVDVDDGHFVNIELK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should contain Virion surface topological domains.2. The protein must exhibit the following characteristics: The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur; Also plays a role in viral proteins secretory pathway; Acidification of the endosome triggers M2 ion channel activity; Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation..3. The protein should be optimized for stability and solubility when expressed in host cell plasma membrane, membrane where it will be involved in protein complex oligomerization. The designed protein sequence is ",
    "response#1": "MEPVDPSLEPWNHPGSQPKTACNKCYCKKCCYHCQKCFLTKGLGISYGRKKRRQRRSTPPSSEDHQNLVSKQPTSQPRGDPTGPEESKKKVESKTETDRLD",
    "response#2": "MKKFLKLISIIILVLTIFSGCSNSAEDTAKKFFESISNDKYDEMYDMLSKDFKSKISKEDFVKQWGNQVGNQIKSIKVLEKEKVNDTYSKVPVHVEYESTMGKYSDTSDVLLVKESGQWKIDYKPS",
    "response#3": "MXKGXKNXXRXXEXAP"
  },
  {
    "reference": "MPGLTIGDTVPNLELDSTHGKIRIHDFVGDTYVILFSHPGDFTPVCTTELAAMAGYAKEFDKRGVKLLGISCDDVQSHKDWIKDIEAYKPGNRVTYPIMADPSREAIKQLNMVDPDEKDSNGGHLPSRALHIVGPDKKVKLSFLYPACVGRNMDEVVRAVDALQTAAKHAVATPVNWKPGERVVIPPGVSDDEAKEKFPQGFDTADLPSGKGYLRFTKVG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in nucleus that is critical for cell redox homeostasis.2. The designed protein should contain at least one Bipartite nuclear localization signal motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "GTLTYIQSRCQEALSNLHTDPETGSHRLVALLPATLQDCEEIHNEVEFEWLRQYWFQGQRYARFCSWWTRPMEQLEKDWKLMETMTMLFLAAVEEEGQAAEGRREMAEILLSALTDRHQQRQTWRDRCHSSLAQTLPPEEAPVDRPFWGVDDPSMPLPFDLADIINRVL",
    "response#2": "GTLTYIQSRCQEALSNLHTDPETGSHRLVALLPATLQDCEEIHNEVEFEWLRQYWFQGQRYARFCSWWTRPMEQLEKDWKLMETMTMLFLAAVEEEGQAAEGRREMAEILLSALTDRHQQRQTWRDRCHSSLAQTLPPEEAPVDRPFWGVDDPSMPLPFDLADIINRVQ",
    "response#3": "GTLTYIQSRCQEALSNLHTDPETGSHRLVALLPATLQDCEEIHNEVEFEWLRQYWFQGQRYARFCSWWTRPMEQLEKDWKLMETMTMLFLAAVEEEGQAAEGRREMAEILLSALTDRHQQRQTWRDRCHSSLAQTLPPEEAPVDRPFWGVDDPSMPLPFDLADIINRVR"
  },
  {
    "reference": "MDQEQTPHSPTRHSRSPPSSASGSTSAEPVRSRWSPKPEQILILESIFHSGMVNPPKEETVRIRKMLEKFGAVGDANVFYWFQNRRSRSRRRQRQLQAAAAAADATTNTCDQTMMVSNSLPHHSGSDLGFGGCSTSSNYLFASSSSSYGGGCDNQSNSGMENLLTMSGQMSYHEATHHHYQNHSSNVTSILCPSDQNSNFQYQQGAITVFINGVPTEVTRGGIDMKATFGEDLVLVHSSGVPLPTDEFGFLMHSLQHGEAYFLVPRQT",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must be able to DNA-binding transcription factor activity.2. The Homeobox; WUS-type domain within the designed protein should be capable of recognizing and binding to a range of DNA sequences. The designed protein sequence is ",
    "response#1": "PDFSGLPSFLPQTPSSRPVTYSYSSNLPPVQPVREVTFREYAIDPTASKWHHPRSNLAHCYSTAEEIMHSRDNSSSVAAEMFGKNAAGVYHHHHHPATPGNTSSNFYSAVGRNGVLPQAFDQFFETAYGTAG",
    "response#2": "PDFSGLPSFLPQTPSSRPVTYSYSSNLPPVQPVREVTFREYAIDPTASKWHHPRSNLAHCYSTAEEIMHSRDNSSSVAAEMFGKNAAGVYHHHHHPATPGNTSSNFYSAVGRNGVLPQAFDQFFETAYGTAR",
    "response#3": "PDFSGLPSFLPQTPSSRPVTYSYSSNLPPVQPVREVTFREYAIDPTASKWHHPRSNLAHCYSTAEEIMHSRDNSSSVAAEMFGKNAAGVYHHHHHPATPGNTSSNFYSAVGRNGVLPQAFDQFFETAYGTAQ"
  },
  {
    "reference": "MELHLVILAAGKGTRMRSALPKVLHKIAAKPMVEHVIDAAVQLPNISSTQLVYGHGGDQMQQALAHRDVSWALQAEQLGTGHAVAQAMPELPDSGKVLILYGDVPLIHTATLERLIAAQPQGGIGLLTVSLPDPTGYGRIVREQGKIVGIVEQKDALPEQLSIKEINTGILVADATDLKYWLSNLSNDNAQKEYYLTDIIEMAHQQGKEIIAAHPASAIEVEGVNNKLQLAQLERAYQLEQAHALMVAGATLRDPSRVDIRGSLTVGQDVEIDVNLIVEGKVILGNNVTIGANCILQDCTIADNTEIKPNSIIEQSIVGKGCSVGPFARLRPQSQMADDSHVGNFVEMKKVQLGRGSKAGHLAYLGDAVIGTEVNIGAGTITCNYDGANKHLTEIGDNAFIGSDSQLVAPVKIGRDVTVGAGSTVTSEVADGELVITRVKQRHIKNWPRPQKIK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein with glucosamine-1-phosphate N-acetyltransferase activity is needed to enhance cell wall organization in a specific context. The designed protein sequence is ",
    "response#1": "RVELAEAEALWQKRKAKELMLSGVTMLRPETLYFSYDTEIARDVIIEPNVFFGRNVKVETGVVIHGFSHLEGAIVKTHSEIGPYARLRPGAILENSSKVGNFCEIKNAKIGEKAKVNHLTYIGDAEIGRGTNIGAGTITCNYDGFNKWKTIIGENAFIGSNSALVAPVEIGKGAYVASGSVITEAVPENCVAFGRARR",
    "response#2": "GVTLKDPARFDVRGEVTTGRDVTIDINVILEGKVVIGDDVTIGPNTIIRDTEIGAGTTIQANCVIEEAVVGANCRIGPFSRLRPGAHLADRVHVGNFVEIKKSQVGEGSKVNHLTYVGDARIGRGVNVGAGTVTCNYDGVNKHPTEIGDAVFIGSGTMLVAPVTIGAGGTIGAGSTITKDTPPDRLTVERSRG",
    "response#3": "GVTLLDPTRIDVRGELVCGRDVSIDVNCIFEGQVELGDNVTVGAHCVLKNVKVAAGTQIAPFSLLEDSTVGEACKIGPYARLRPGAALAGHVHIGNFVEVKKSTIGEGSKVNHLTYVGDASIGSRVNVGAGCVTVNYNGVDKFRTVIEDNVFVGSGTLMVAPVTLEAGSTVGAGSVVTKNAPAGELTVARARR"
  },
  {
    "reference": "MDVHYDADPALIHDKQVAVIGYGSQGHAHALNLHDSGVDVAVGLRPGSSSRPKAEQQGLTVMDIGEAAAWGDVVMLLIPDQHQKDVYEAKIAEHMTPGTALGFGHGFNIHYDRIEPPEAVDVFMVAPKSPGHLVRRTYTDGSGVPCLAAVDQDPSGGAMALAISYADAIGGTHAGVIETTFKDETETDLFGEQAVLCGGSQALIQAGFETLVDAGYPEELAYFECLHELKLIVDLYYEGGLEYMNHSVSDTAEYGGYTRGPRVIDDAVREQMQEILEEVQSGEFADEWIEEYEQGAPQLQEERAALTEHPIEQVGQTLRGMMPWLNGDETSADEDAPDAADTAPASSS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have a high affinity for substrate, NADP(+).2. A protein with magnesium ion binding is needed to enhance valine biosynthetic process, isoleucine biosynthetic process in a specific context.3. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.4. For general function, the protein need meet that Involved in the biosynthesis of branched-chain amino acids (BCAA); In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB); Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate5. Ideal protein should have a binding affinity for key enzymes in the L-valine biosynthesis, L-valine from pyruvate: step 2/4 pathway to enhance the overall efficiency of the pathway.6. The designed protein have KARI C-terminal knotted, KARI N-terminal Rossmann domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MATIYYDKDADLKALKGKTVAIIGYGIQGRGQALNLRDSGVKVIVAQRPGGPNFDLAKQDGFTPLSAAEATKKADVVILLAQDMLQGDIYREAIAPNLRPGQTLGFSHGFAVLYGLIKPPKNVDVVMVAPKGPGSLVRSQYLEGKGVPALVAVYQDASGKAKATALAWAKGIGATRAGVLETSFIAEVKSDLMGEQTILCGMLQTAAVLGHKQLIAQGMDAAYARKLLQYGLETTTEGLKHGGITNMMDRLSNPAKILAFDMAEELKVILRPLFQKHMDDIIEGRFSATMMADWANDDVNLLTWRAETAETSFEKSPECDTEISEQVRWSFVAEVKSDLMGEQTILCGMLQTGSLLCYERLVAEGADPAWAGKLIQFGWETITEALKQGGITLMMDRLSNPAKLRAYTLAGQLKTLMAPLFAKHMDDILSGAFSQGMMADWAADDAHLLTWRAETGESAFEKAEYYDKGIFVVAMIKAGVELAFDAMVDSGIINASAYYESLHETPLIANCIARNMLHEMNVVISDTAEYGNYLFTHAAVPLLADYTASLTLEQLGEGLKESSNNVDNARLIEVNEAIRSHGVEVIGKELRGYMTDLR",
    "response#2": "MFYDDNADLSIIQRRKVGVIGYGSQGYAHSLSLRDSGVQVRVGLPEGSKSRPKAFEQGLDVDTPAEVAKWADVIMLLVPDTAQADVFENDIEPNLQPGDALFFGHGLNIHFELVKPSGDVTVAMVAPKGPGYLVRRQFVDGKGVPCLIAVNQDPTGTGEALALSYAKAIGGTRAGVIKDFIAEVKSDLMGEQTILCGMLQTSAILGYEKMVADGVEPGYAAKLIQQGLETITEALKQGGITNMMDRLSNPAKIKAFDMAEELKGILAPLFEKHMDDIISGEFSRTMMADWANDDANLLRWRAETNETAFENFKEETETDLFGEQAVLCGGTSALVKAGFETLVEAGYQPEIAYFECLHELKLIVDLMYEGGLEYMRYSISDTAQWGDFQAGPRIVTDETKAAMKEILSDIQKGKFAKGWILENKLNRPEYNAINEAEKNHPLEVVGRELREMMESLEMYYDKGIFVVAMIKAGVELAFDAMVDSGIINASAYYESLHETPLIANCIARNMLHEMNVVISDTAEYGNYLFTHAAVPLLADYTASLTLEQLGEGLKESSNNVDNARLIEVNEAIRSHGVEVIGKELRGYMTDLTISPKVTE",
    "response#3": "MFYDDNADLSIIQRRKVGVIGYGSQGYAHSLSLRDSGVQVRVGLPEGSKSRPKAFEQGLDVDTPAEVAKWADVIMLLVPDTAQADVFENDIEPNLQPGDALFFGHGLNIHFELVKPSGDVTVAMVAPKGPGYLVRRQFVDGKGVPCLIAVNQDPTGTGEALALSYAKAIGGTRAGVIKDGFLGKCRFMKREEFNDGCNFIKDWNIVIVGCGAQGLNQGLNMRDSGLNISYTLRDAAISEKRQSWQWATENGFTVGSYAELIPQADLVLNLTPDKQHTSAVTAVMPLMKQGATLAYSHGFNIVEEGMQIRSDITVVMVAPKCPGTEVREEYKRGFGVPTLIAVHPENDPQGNGLAIAKAYASATGGDRAGVLENCTIMSNEHIDEQTYFDKGIFLVAMIKAGVELAFDTMVDAGIIEESAYYESLHETPLIANTIARKRLYEMNVVISDTAEYGCYLFNHAAVPLLRDYVKSMSAEYLGGGLKDSSNAVDNVRLIEVNQAIRNTAVEFIGEELRGYMKDIMKLKD"
  },
  {
    "reference": "MSLFRIFINQLEEDDEDMATSKKMITREEWEKKLNAVKLRKEDMNTLVMNFLVTEGYVEAAEKFQRESGTKPEIDLATITDRMAVKKAVQNGNVEDAIEKVNDLNPEILDTNPELFFHLQQQRLIELIRQGKTEEALEFAQEELAPRGEENQAFLEELEKTVALLVFDDASTCPVKELLDLSHRLKTASEVNAAILTSQSHEKDPKLPSLLKMLIWAQTQLDEKAVYPHINDLSTGKLEDPSE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein contains novel CTLH, LisH domains that confer a unique function or activity.2. The protein should have a specific function in proteasome-mediated ubiquitin-dependent protein catabolic process and should be localized to nucleus, cytoplasm in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "APKGVLLYGPPGTGKTLLARAIASNLEANFLKVVSSAIVDKYIGESARVIREMFGYARDHQPCVIFMDEIDAIGGRRFSEGTSADREIQRTLMELLNQMDGFDTLSKVKIIMATNRPDVLDPALLRPGRLDRKIEIPLPD",
    "response#2": "GKLMDAMAHAKRYLTPYTETQSKEIHRAAGLLAFPQDTKAEPYKLTDLQSMYSFDRWNYLSDLFIRTHHELLSLPSSPLLHIALSAGLSALKTPSCHSAC",
    "response#3": "GKLMDAMAHAKRYLTPYTETQSKEIHRAAGLLAFPQDTKAEPYKLTDLQSMYSFDRWNYLSDLFIRTHHELLSLPSSPLLHIALSAGLSALKTPSCHSACA"
  },
  {
    "reference": "MAHFNECAHLIEGVDKAQNEYWDILGDEKDPLQVMLDMQRFLQIRLANVREYCYHPDKLETAGDVVSWMREQKDCIDDEFRELLTSLGEMSRGEKEASAVWKKWKARYIEAQEKRIDEMSPEDQLEIKFELVDIFHFVLNMFVGLGMNAEEIFKLYYLKNKHNFERQDNGY",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The desired protein should be able to perform dCTP + H2O = dCMP + diphosphate + H(+) The designed protein sequence is ",
    "response#1": "LTNLYPILVRPYVPAVIPLGIRIIYGNKGQGFILAGSSQKKVFCHTGLIDPGYRGEIKLIVLNTTKYNVTLFAR",
    "response#2": "LTNLYPILVRPYVPAVIPLGIRIIYGNKGQGFILAGSSQKKVFCHTGLIDPGYRGEIKLIVLNTTKYNVTLFAK",
    "response#3": "LTNLYPILVRPYVPAVIPLGIRIIYGNKGQGFILAGSSQKKVFCHTGLIDPGYRGEIKLIVLNTTKYNVTLFAL"
  },
  {
    "reference": "MSLPLHVVILAAGAGKRMKSAKPKVLQPVAGRPMLAHVIETARQLQPAAIHVVYGHGGDAVRAAFGAQPDLLWAEQAQQLGTGHALQQAMAAVPDAATVLVLYGDVPLIRADTLLRLLHSPGRLAVLVAEPEDPSGYGRIVRDAAGKVAAIVEHKEADHEQRRIRIINTGIVTAESTALKRWLAQLRSDNAQGEYYLTDVFAAAAAEFTPAEMVLVADPTEAEGANDPWQLAQLERAWQLRAARALCEQGAHLLDPNRLDQRGRVRVGRDVCIDVNVVLEGEVELGDGVSIGPFVRLKDVVLGPGTEVRAHCDLEGVITEGAVLIGPFARLRPGTVLADGVHIGNFVETKQAVLGVGSKANHLSYLGDATIGSGVNIGAGTITCNYDGVNKSQTAIGDDVFVGSNSALVAPLTIGAGATIGAGSVITRDAPAGQLTVARARQASHEDWKRPKKK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should consider the endogenous proteins and enzymes present in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway to avoid interference with native metabolic processes.2. The designed protein should have a binding site that is specific to the target Mg(2+), acetyl-CoA ligand.3. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc); The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain. The designed protein sequence is ",
    "response#1": "AVVLAAGKGTRMKSDLPKVLHRLAGAPMLAHVLGAAASAGVKRAALVIGPGMEAVGEAGLSVVPEAQVFVQADQMGTADAVKSAAPAYEGADGPVLVLYGDTPLLRPETLKAVLGELEAGADLVVIGFEAADPTGYGRLLFDDKSRLAGIREEKDLVARIVEHKDATEAERAIRLCNSGMMAVRGADLLRLLDRIGPANSKGEYYLTDIVAIARADGLSAAAVDCDEAQVLGANDRVQLAEAEAAFQARMRARARAMEDGVSMIAPDTVFFSADTELGPDVSVGPYVVFGDGVRVFSGAEIRAFSHLEGCVVHAGAVIGPYARLRPGAEIGAGAHVGNFVEVKAASLGAGTKANHLSYIGDAQIGPGTNIGAGTITCNYDGKAKHETKIGAGVFVGSNSSLVAPISIGDGALIAAGSTLTEDVAPGALAIGRARQRNIEKWRRAGRAAKAAKGAAKK",
    "response#2": "MTELVLAAGAGTRMKSDTPKVLHTLAGRSMLSHSLHAVAKVAPQHLVVVVGRDRERVAPAALEIGQELGRPVEIAVQEQQQGTGHAAECGLSALPDDFTGVVVVTAGDVPLLDADTLADLIATHSAEAAVATVLTTTLADPTGYGRVLRTQDREVIGIVEQADASPSQQAISEVNAGVYAFDIAALRSALSRLRSDNAQHELYLTDVISIVRSDGEVLGIVEYTDASEAQVRLGEGARIGAYCHIANAEIGAGAVIHPYTHIVDAHEHTVLGVNSRVQLAEAGRILRRRINTAHMRAGVTIVDPTSTWIDVDVKLAADVTLLPGVQLLGATVVAEDAVIGPDSTLKDVEVGAGARVVRTHGELAVIGAGANVGPFSYLRPGTELGAGGKIGAFVETKNAQIGDGAKVPHLSYVGDATIGEGANIGAGTIFANYDGVEKHRTVIGAHAKTASNNTFVAPVTVGDGASTGAGSVIRRDVPPGSLAVTSGPGVTIVDPASTWVDVDVRIGRDTVVRPGTQLLGATHIGGRCTIGPDTTLTDVTVGDGAEVVRTHGSSSVIGDDAVVGPFTYLRPGSVLGVAGKLGAFVETKNATIGDGTKVPHLTYVGDADIGEHSNIGASSVFVNYDGEAKRRTTIGSHVRTGSDTMFVAPVTIGDGAYTGAGTVIREDVPPGALAVSAGPSVVPGVKKKRQDDDEDEN",
    "response#3": "VVMAAGRGTRMKSRIPKVLQRLAGRPLLHHVLDQAAHLAPRRVVLVAGHGAMEVEASCQAFTPAEGLKDLKSVRQEPQLGTGHAVQQALPVLAGDGTVLVLSGDVPLTRAETLAALVAAAQPERLALLTVELDDATGYGRIVRDASGAVTAIVEHKDASEAQRAIREINSGVLAAPTRQLRDWLARLDNNNAQGEYYLTDIVEMAVRDGVAVHASQPRDAIEVEGVNNRLQLAALERAHQARVAERLMLAGVTVVDPATTWIDASVTFEPDAVVHPGTQLHGATRVAAGAEVGPNSTLTDTAVGPGARVVNTVAVGAEIGPEASVGPYAYLRPGTRLARKAKAGTYVEMKNAEIGEGTKVPHLSYVGDASIGEYTNIGAASVFVNYDGESKHRTTIGSHCKTGSDNMFVAPVTVGDGAYTAAGSVITKDVPPGSLAVARGQQRNIDGWRRPAKK"
  },
  {
    "reference": "MKDIVSQVKDIAQKICEEFHLELFDVKYYNKSGRWFLEIIIDNPYDYISTKDCENVSRKLEFELDKINIIPNKYYLTVSSPGLNRPLRNVKDFERFTGKKAKIKTRENTYIGYIKNVVDNIITFETDGKFIEFKFEEIKKANLEIDI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Required for maturation of 30S ribosomal subunits. The designed protein sequence is ",
    "response#1": "PKILITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSLQKGTFS",
    "response#2": "PKILITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSLQKGTFR",
    "response#3": "PKILITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSLQKGTFV"
  },
  {
    "reference": "MMRLSKLFSRCMTFGHYEEKLQFCVWSAEWLRYAEVEAAREHKITLYDLMQRAGKAAFDVFRREYPSSRHWLILCGSGNNGGDGFVVARLAADDKIRVTVLAVKGKNPLPPEATAAHEALVKRGGVEIHDAGQWNEKDLAEDVDLVVDGLLGTGITGAPRTDYARLIDLANALPVPRVSIDIPSGLNAETGAVEGVCVRAEHTVTFIALKPGLLTGRAREYVGTLHYDCLQLGEWLLHPKRQGELFCRRIVSSHLKTIFAQKRSPCAHKGTNGKLLLVGGDHRFGGAIIMAAEAALHAGVGLVCVVTRSVHIGPLLSRCPEVMVYDYEKCPLRELMGWSTCVAVGPGMGQVGWGETLLRQILRCRMSLEEKPFLLDADALNILAKFVSSGAAKKEGLLPSNNNKMVITPHPGEAARLLQCTIEEVERNRFQAAKKLADSYGCTVLLKGPGTVIYTAEDANISVDALNNTHGILSSRFVVSDAGNSGMAAGGFGDVLTGVISGCLAQGFPVWQATCGGCLAHSTAADLAAAHKDVRGLRATSLFEHISTCVNPLTERQCESQGPEKKSKVEKSE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein with ATP binding, NADHX epimerase activity, ATP-dependent NAD(P)H-hydrate dehydratase activity functions is required to enable nicotinamide nucleotide metabolic process.2. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration The designed protein sequence is ",
    "response#1": "SRERRADTHKGSYGHTLIAAGTKRMSGAGLLCAKAALRAGCGLATWAVPASLATHLLGRVPEAMLAGLADAGHGDWTHVNAEAILALAEDCDALALGPGMGRYEGDSQWLRGLWEGAGCPLVLDADALNMLAEAPDFADWQPRRAATVLTPHPGEMARLAGVSTREVQRDRIGLARRYAEQHGVTLVLKGARTVVATPDGAVYVNVNGNAGMATAGAGDVLAGIIASLLSQGFDAAQAACLGVYMHGAAGDRAALKRTSPASLLAGDLLEA",
    "response#2": "ELNRPSAAALARSRDSHKGSYGAAAVIGGAPGMLGAALLAGRSALAGGAGKVYLCPLDGRLPVDPAAPELMVRPVDEAAELPAADTFAVGPGLGQQEQAARLLEQAIARPCPLVLDADALNLLAARDELAERVAARAAPTVLTPHPAEAARLLRSSVGEVQSDRVRHVRRLAERFNCVAVLKGAGSLIARPDGFYRVNTSGGPELASAGQGDVLTGLIAALLAQGLDAFDAASLAVRLHGLAGDDYRAENGGPIGLAASATTPRVSRALNRR",
    "response#3": "WPPPRPVDAHKGTFGHALLLAGSPGYHGAAVLAARAAQRARPGLISLFTDAAVYGPVASQLQAVMVHAVGDNRFNLPEKGTALLVGPGLAAPNAKNRFAEQLLSAWRTTHHPVVVDASALDWLAPRHSPPRENIRVLTPHPGEAARLLRSSAGQVQADRLQALRTISAQRGHCWVILKGRHTLIGRSEGPVYVNGSGNPDLAQGGAGDILAGLLVGYLAQPELQADPARTLALAVWQHGEAADRLTAAGRRWVPEEV"
  },
  {
    "reference": "MVESGIHDTLCRAIIALFIPVNIKGEFNTSFKKLENLTRPFVNYFILPLFVFMNSGILLEYFAFKGICSNSILALIYGIIFGLFVGKQLGIMLFSYPFVKFKLCNLPSDTSWLKFYSIAILGGIGFTLSLFIGSILRLRAAALQTL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in plasma membrane that is critical for regulation of pH. The designed protein sequence is ",
    "response#1": "PGKPEILKCRSPEKETFTCWWKPGDDGGLPTNYTLTYHKEGETLTHECPDYITSGPNSCYFNKKHTSIWTMYIITINATNQMGSC",
    "response#2": "PPGKPEILKCRSPEKETFTCWWKPGDDGGLPTNYTLTYHKEGETLTHECPDYITSGPNSCYFNKKHTSIWTMYIITINATNQMGSSSSDPRYVDVTYIVEPQ",
    "response#3": "PPGKPEILKCRSPEKETFTCWWKPGDDGGLPTNYTLTYHKEGETLTHECPDYITSGPNSCYFNKKHTSIWTMYIITINATNQMGSSSSDPRYVDVTYIVEPN"
  },
  {
    "reference": "MANTNSYLIVEPAAFIKGEVTVPGDKSISHRAVMLASLARGRSSIRNFLPSEDCLCTLRAFEAMGVSVEKQGKTHLIIEGKSGVLFPPFEAIDCGNSGTMMRLLAGILTAQPFYSKLIGDYSLSKRPMSRIIEPLRMMGAKIRADGPKDTPPLIIEPSTLKGIEYRLPIPSAQVKSCILLAGLFASGVTRIIEPVASRDHTERLLEQFGVRTIREKGEIILHGGMKLEATDIEVPGDFSSAAFWIGAAAAIPHSELRVRSIGLNPTRTGLLNILLRMGAQIEESIESYSKGEPIGSLWVRGNKLRATVIQGEEIVGVIDEIPILAVVGALADGVTEIRNAQELRVKESDRIKAIVTNLRAFGVEVEELVDGMRIKGKNTVQAARVESFGDHRIAMAFSILALRANKASRIDDVDCIATSYPGFQEDLRALTQSSTEGWAEETKKIKNFIMGSQKFAKATELEKKELPYPVITIDGTTASGKSTVAKEIAKVLGFTYFNTGALYRAVTWKILALGYDVHDEEKAVEACRKLNLRCVIRKEDSFSQASILVDDQQPLEKELRSPEVNEAVSIVASYRKIREWLLPFQRELAYKEPLVVEGRDIGTVVFPESPYKFFLDANLEEREKRREEQGERDNVAQRDRLDILRSSAPLLCPVDAVKLDTTKPSAQKVVEKILDILWKKGLTISRGKMD",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be able to compete with endogenous proteins or inhibitors for phosphoenolpyruvate, 3-phosphoshikimate, ATP binding.2. The Proton donor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The designed protein should have CMP kinase activity within cytoplasm to regulate pyrimidine nucleotide metabolic process, aromatic amino acid family biosynthetic process, chorismate biosynthetic process, amino acid biosynthetic process, phosphorylation.4. A protein that can modulate the overall flux of metabolites through the chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7, Metabolic intermediate biosynthesis, chorismate biosynthesis pathway, enhancing or suppressing its activity as needed.5. The designed protein should contain one or more Enolpyruvate transferase, Cytidylate kinase domains that are essential for its function.6. For general function, the protein need meet that Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. The designed protein sequence is ",
    "response#1": "QGRVVLPGSKSISNRVLLLAALCRGRTTLHDLLDSDDTRVMLAALRQLGCDVQQDGTTVTIDGLDAKLPATVLKFFMGNAGTAMRPLTAALAVLGGDFELSGIPRMHERPIGDLVDALRQLGCQIDYLGNDGYPPLRIGRPTLKLDAPIKVRGDVSSQFLTALLMALPLVASRDVHIEVVGELISKPYIEITLNLLARFGIAVRREGWQRFTIPGGSRYQTPGVLHVEADASSASYFIALGAIAANQATPLRIEGVGESSIQGDIKFVDAARLMGARVTSGPNWLEISKGSWPLKAIDLDCNHIPDAAMTLAVMALYADGPTTLRNIASWRVKETDRIAAMATELRKLGATVEEGPDFIRIQPLPQATGWKAASIHTYDDHRVAMCFSLAAFNPAQLPVRILDPKCVGKTFPDYFEALFSVVPQ",
    "response#2": "MSSICIDGPTASGKGTLAAEVAQRLGYHLLDSGALYRLVGLAAGKAGLSTAEDDLRQPEQAQRLGTLAAGLQVRFDGPRIWLDGVDVSDALRSEVAGMAASRVSAVPEVRAALLTLQHGFRRLPGLVADGRDMGTVIFPDAPLKVYLTASAEQRAQRRYKQLISKGIAANITSLLADLEKRDHRDSSRAHAPLKPAEDALQLDNSGLDVEQSVQQVLAWCHRVWRSQPVIAIDGPTASGKGTVAAHLAQALGFHYLDSGVMYRATALLAQRQGIALDDEAALAALARHLPIRCEGGTVWLGPENASDAIRTEAVGQAASTLSALPAVRRALLDLQRSLRRAPGLVADGRDMGTVVFPDARLKVFLTADAQARAERRHKQLISKGISTTLTQVLHELEQRDARDTQRSVAALKPAQDALPLNTTGMTIDEAVDAVLAQVICIDGPTASGKGTLAALTAQRLGYHYLDSGTLYRLSAFVAARAGVALEDGDGVAQLARAMPVHFQGDRVFLGDEDVSEAIRTETAGMAASKVSAHPQVRNALLALQQSFRRLPGLVADGRDMGTVIFPDAQLKIYLTASARHRAERRHKQLISKENQTTITAIQQALEARDASDMSRQTAPLKPADDARLLDNTGMTIDEAVDAVLAQVVAIDGPSASGKGTVGARVAAALGWHHLDSGSLYRLVALSAERGGIDLDDEVALAAIAGDLPARFEGERVWLGTDDVTDAIRSEQCSAGASKVAVLPAVRAALLGRQRDYRQGPGLVAEGRDIGSVIFPDASVKIFLTASVEARAERRYKQLIGKGLAANMESLMQDLRERDARDAARTVAPLQKLPDAALLDT",
    "response#3": "MTNCIDGPTASGKGTLAARVAQQLGYHLLDSGALYRITALAAARAQLPLDTSGAAALARLAQELPIAFDGDRVLLGGEDITEAIRSEDVGMNASRISALPAVRAALVDLQHSFRQLPGLVADGRDMGTVIFPDAPLKVFLTASAEKRAQRRHKQLISKGISTTLAALCADLQARDARDTSRSASPLQPAPDALLLDNSDLSIEESLTQVLAQIAPLLTTGAGEVHLPGSKSISNRVLLLAALSHGTTTVHDLLASDDTRVMLDGLRQIGCTVDERGSTVLITGLGGGQPAAPAKLFMGNAGTAMRPLTASLALLGGEYELSGVPRMHERPIGDLVDALRQLGCQIDYLGNDGYPPLRIAHAGGVPELLLDAPIRVRGDVSSQFLTALLMALPLAAGQRAIEIEVVGELISKPYIAITLQLLERFGIRVAHDHWQRFSIPAGSRYQSPGEIHVEADASSASYFIALGAISTSARGQNDPKTSAEGPGGITVRGVGLDSIQGDIRFVDAARAMGAVITGGPNWLNIRRGAPGHGWPLKAIDLDCNHIPDAAMTLAVMALYAEGTTTLRNIASWRVKETDRIAAMATELRKLGATVEEGADYIRVTPPATLQDWKPASIHTYDDHRVAMCFSLAAFNPAQLPVRIEDPKCVAKTFPEYFETLFAAVEKSG"
  },
  {
    "reference": "MSINQQYVSGKSTEVRDVTIIGGGPTGIFAAVQCGMHHINCRIIDSMPSLGGQLTALYPEKHIYDVAGFPEVSAAGLIEQLWSQAARYKPEVVLGDKVVDVKKLDDGSFEVFTEKGHSYFSRAVLIAAGLGAFSPRKLPQLKDFEHLEETSIFYTVSSIDHFKDEKVVVVGGGDSALDWTIALLNVAEHVTLVHRMKEFQAHGKTVADAYEAQETGKLDIYLESEVASVLADSDRLTHAILKTPDEEITIEATRLLPLIGFRSNLGPIKNWGIEISGNGILVDNHMQTTVEGIYAAGDIAVYEGKLKLIQTGLSDAAMAVRHSLRYIKPGEKVKQQFSSQKASEKKA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a high specificity for FAD binding, with minimal off-target effects.2. The protein have enhanced H(+) + NADP(+) + 2 reduced [2Fe-2S]-[ferredoxin] = NADPH + 2   oxidized [2Fe-2S]-[ferredoxin].3. The protein requires FAD to activate a critical catalytic site.4. The protein must be able to NADP binding, ferredoxin-NADP+ reductase activity. The designed protein sequence is ",
    "response#1": "DAVVIGAGPVGLFQVFQLGLQGIAAHLVDALPHVGGQCAELYPDKPIYDIPGIPVCTGLGLVELLQRQIAPFAPTLHLGQQIHALAAQADGRILLTTTAGTALLARSVFIAAGVGAFVPRAIKAEGVEALAPGQLLYHPDAATATRAATGKRVVVHGGDEAAVQAALDCVDAAAQVLLLHRRDAFQAAPAPLAQLQALREAGRIQVVIGQITGVETAPDGTLQALALLDPQGQPQRQPLDLLLAYLGISPRLGPIADWGLAMDRKQLAVDTATFATSVPGIYAVGDIL",
    "response#2": "DAVVIGAGPVGLFQVFQLGLQGIAAHLVDALPHVGGQCAELYPDKPIYDIPGIPVCTGLGLVELLQRQIAPFAPTLHLGQQIHALAAQADGRILLTTTAGTALLARSVFIAAGVGAFVPRAIKAEGVEALAPGQLLYHPDAATATRAATGKRVVVHGGDEAAVQAALDCVDAAAQVLLLHRRDAFQAAPAPLAQLQALREAGRIQVVIGQITGVETAPDGTLQALALLDPQGQPQRQPLDLLLAYLGISPRLGPIADWGLAMDRKQLAVDTATFATSVPGIYAVGDIDAVVIGAGPVGLFQVFQLGLQGIAAHLVDALPHVGGQCAELYPDKPIYDIPGIPVCTGLGLVELLQRQIAPFAPTLHLGQQIHALAAQADGRILLTTTAGTALLARSVFIAAGVGAFVPRAIKAEGVEALAPGQLLYHPDAATATRAATGKRVVVHGGDEAAVQAALDCVDAAAQVLLLHRRDAFQAAPAPLAQLQALREAGRIQVVIGQITGVETAPDGTLQALALLDPQGQPQRQPLDLLLAYLGISPRLGPIADWGLAMDRKQLAVDTATFATSVPGIYAVGDITSADALQAVRRDAVVIGAGPVGLFQVFQLGLQGIAAHLVDALPHVGGQCAELYPDKPIYDIPGIPVCTGLGLVELLQRQIAPFAPTLHLGQQIHALAAQADGRILLTTTAGTALLARSVFIAAGVGAFVPRAIKAEGVEALAPGQLLYHPDAATATRAATGKRVVVHGGDEAAVQAALDCVDAAAQVLLLHRRDAFQAAPAPLAQLQALREAGRIQVVIGQITGVETAPDGTLQALALLDPQGQPQRQPLDLLLAYLGISPRLGPIADWGLAMDRKQLAVDTATFATSVPGIYAVGDI",
    "response#3": "DAVVIGAGPVGLFQVFQLGLQGIAAHLVDALPHVGGQCAELYPDKPIYDIPGIPVCTGLGLVELLQRQIAPFAPTLHLGQQIHALAAQADGRILLTTTAGTALLARSVFIAAGVGAFVPRAIKAEGVEALAPGQLLYHPDAATATRAATGKRVVVHGGDEAAVQAALDCVDAAAQVLLLHRRDAFQAAPAPLAQLQALREAGRIQVVIGQITGVETAPDGTLQALALLDPQGQPQRQPLDLLLAYLGISPRLGPIADWGLAMDRKQLAVDTATFATSVPGIYAVGDITDVSSRT"
  },
  {
    "reference": "MSDSIYAPHNKHKLEAARAADAAADDAATVSALVEPTDSTAQASHAAEQTIDAHQQAGDVEPERCHPHLTRPLLYLSGVDATMTDKELAGLVFDQVLPVRLKIDRTVGEGQTASGTVEFQTLDKAEKAYATVRPPIQLRINQDASIREPHPSAKPRLVKQLPPTSDDAFVYDLFRPFGPLRRAQCLLTNPAGIHTGFKGMAVLEFYSEQDAQRAESEMHCSEVGGKSISVAIDTATRKVSAAAAEFRPSAAAFVPAGSMSPSAPSFDPYPAGSRSVSTGSAASIYATSGAAPTHDTRNGAQKGARVPLQYSSQASTYVDPCNLFIKNLDPNMESNDLFDTFKRFGHIVSARVMRDDNGKSREFGFVSFTTPDEAQQALQAMDNAKLGTKKIIVRLHEPKTMRQEKLAARYNAANADNSDMSSNSPPTEARKADKRQSRSYFKAGVPSDASGLVDEEQLRSLSTVVRNELLSGEFTRRIPKVSSVTEAQLDDVVGELLSLKLADAVEALNNPISLIQRISDAREQLAQKSASTLTAPSPAPLSAEHPAMLGIQAQRSVSSASSTGEGGASVKERERLLKAVISVTESGAPVEDITDMIASLPKKDRALALFNPEFLKQKVDEAKDILDITDESGEDLSPPRASSGSAPVPLSVQTPASAIFKDASNGQSSISPGAAEAYTLSTLAALPAAEIVRLANSQSSSGLPLPKADPATVKATDDFIDSLQGKAAHDQKQKLGDQLFKKIRTFGVKGAPKLTIHLLDSEDLRALA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein contains novel RRM domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "IRQGSPTALRAELWALILNISSQPEDVLYYEQLKTNVIQHDLLVDSLIYKDVKLTASNDDYYFVFEDYLYQVLLCFSRDTSVLSHFAFNSASPPKSYIRGKLGLEEYAVFYPPNGVIPFHGFSMYVAPLCFLYHEPSKLYQIFREMYVRFFFRLHSISSHPSGIVSLCLLFETLLQTYLPQLFYHLREIGAQPLRISFKWMVRAFSGYLATDQLLLLWDRILGYNSLEILAVLAAAVFAFRAVNLMEVI",
    "response#2": "MTTEQRRNLQAFGDYVRKILDPTYILSYMTSWFREDEVQCIQAERNNKGPTEAATLFLKYLLELQEEGWFRGFLDALAHAGYSGLYEAIESWF",
    "response#3": "MRRRVLVAATSVVGAVGAGYALVPFVASMNPSARARAAGAPVEADISKLEPGALLRVKWRGKPVWVVHRSPEMLAALSSNDPKLVDPTSEVPQQPDYCKNPTRSIKPEYLVAIGICTHLGCSPTYRPEFGPDDLGSDWKGGFHCPCHGSRFDLAARVFKNVPAPTNLVIPKHVYLNDTTILIR"
  },
  {
    "reference": "LEFAAGIPGSVGGAVYMNAGAHGSDISRVLVKALILFEDGTIDWLTNEEMAFSYRTSILQNERPGICLEAVLQLEQKERDEIVAQMQKNKDYRKETQPVSNPCAGSIFRNPLPEHAGRLVDEAGLKGHQIGGAKVSEMHGNFIVNAGGATAQDVLDLIAFIQKTIKEKYDIDMHTEVEIIGEKR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be optimized for activity in peptidoglycan biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. For general function, the protein need meet that Cell wall formation.3. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in regulation of cell shape.4. The Proton donor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "VMHAIIDIRQQKLPDPKQLPNCGSFFQNPIIPQDQFTALQSSYPAIVGYPMPDAMIKVAAGWLIEQAGLKGGGIEPIVTHQQQALVLTNHTPYQATQKEVAAAQQYITDTVYERFAIPLSREPVWI",
    "response#2": "IAGMHGKTTTSAMTAHVLREGGLHPSHYVGAESPILGTNAHWDPRGEYFVAEGDESDGTLLCFHPEHSLILNIEEEHLDFYADLAAIEEVFARLIEQTTGTVFYDVDDTNAARLCANRKEAISFGFSEKADYRAAEIELRDFASVFCVYRRGERLGEAILNVPGEHNVQNAAGVIALAEL",
    "response#3": "LRGGNPLIGTVRVSGAKNSALPCMAAAILTEDEVILENIPQVRDIETERKLLTSMGAEVELGYGRAQHRTTISCRVLSDPTAKYEIVKTMRASALVLGPLVARTGLARVSMPGGCAIGARPIDLHIKGLEQMGATIVYEHGYIEARAERLKGAQIHFDKITVTGTEDLMMAAVLAEGETVLENAAREPEVTDLAALLTAMGAQIEGAGTSEIRIQGVEKLHGARHRINPDRIEAGTFLIAGAITGGDLCVSHCNPAHLGAVIAKLEEAGVRIDVLGKDSLRVRSEGHLKPIDVSTEEYPGFPTDMQAQYMALATQAEGTTLVKENIFENRFMHVQELVRMGANIKTAGRIASVRGKTPLSAAAVMCSDLRASASLVLAALVANGESILDRVYNIDRGYEHIEEKLRGLGARIERVT"
  },
  {
    "reference": "MLRLTNRQILLGITGGIAAYKSAELTRMLKKAGADVRVVMTPAAAEFITPLTLQALSGNPVHLQLLDPEAEAGMGHIELARWADLILVAPATADFMARVAAGMGNDLLTTLCLASDAPLCLAPAMNQAMWRDQQTQLNVQALQARGIQLFGPASGEQACGDTGIGRMLEPEALLQAIANQFDYDLLNGKRIVITAGPTREAIDPVRFISNHSSGKMGYALAEAAADAGAEVILISGPVNLHAPAHVKRIMVESAQDMLDAALHTADNCDVFIGAAAVADYRPEFVSTNKLKKGKEESMEIRLIKNADIIATIAALPNKPFTIGFAAETNDVLNYAREKRLRKQLDMIIANDVSIAGIGFNSDENQVTLITSTDEISLPQTSKRQLARQLIEHIAPHLPTTR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be optimized for FMN binding, phosphopantothenate--cysteine ligase activity.2. The protein with the reaction H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2, the Proton donor must be optimized to ensure efficient catalysis.3. The protein design should take into account the physiological conditions of the Cofactor biosynthesis, coenzyme A biosynthesis pathway.4. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.5. The protein contains novel Flavoprotein domains that confer a unique function or activity.6. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.. The designed protein sequence is ",
    "response#1": "MNILLGVSGSIAAYKSPDLVRRLKEVGFDVRVVMTDGGRSFVTPLTLQAVSGYPVVYDLLDPAHEAGMGHIELARWADLILIAPTSANMIAKLAGGFADDLLSAICLATEKPIMLAPGMNRLMWANQAVQENVAKLEARGMILLAPESGSQACGEIGQGRMPEPLQIRDAQVLAETLRAGKTVLITAGPTREALDPVRFISNRSSGKMGYALASAARQAGAEVILVSGPVSVTVPDGVKCINVESAQQMFAATHEHIEGVDIFIAAAAVADYRPANSAEQKIKKTASEMSVELVRAPDILASVAKINNGPFAVGFAAETENLRDYARTKLDKKNLDMIVANLVGENLGFDADENAVEILWRDGHQELPRMAKRDLARRLVELIAERYGP",
    "response#2": "MNILLGVSGSIAAYKSPDLVRRLKEVGFDVRVVMTDGGRSFVTPLTLQAVSGYPVVYDLLDPAHEAGMGHIELARWADLILIAPTSANMIAKLAGGFADDLLSAICLATEKPIMLAPGMNRLMWANQAVQENVAKLEARGMILLAPESGSQACGEIGQGRMPEPLQIRDAEGILAASLDLSGKKFLITAGPTREYIDSVRFISNPSTGKMGFALAEMAKKFGADVLLISGPTELVPPHGVETIEVVSAEEMHDAVLKHFDNADIIIMASAVADYTPSKKFERKIKKTSDELELKLQRTPDILAELGRQKGDKILVGFALESDYLIENARKKIEEKNLDLIVANYIGEQTGFASDTNEVVLLRDGSETVLPKMSKRELAAAILDEVERLL",
    "response#3": "MNILLGVSGSIAAYKSPDLVRRLKEVGFDVRVVMTDGGRSFVTPLTLQAVSGYPVVYDLLDPAHEAGMGHIELARWADLILIAPTSANMIAKLAGGFADDLLSAICLATEKPIMLAPGMNRLMWANQAVQENVAKLEARGMILLAPESGSQACGEIGQGRMPEPLQIRDGQLLQAIESYFLEGKKVLITAGPTREAIDPIRYLTNHSSGKMGYAIAEAASARGADVVLVSGPVNLPAPPGVTVIDVESTADMFGAVKGHMDADLGIFTAAVSDFTPRDVQSSKMKKSEDDDELSIELKKTQDILQYAGDHKGDMFVVGFAAETDNVRKYAAAKLEKKNADCIVMNDVSDKEIGMNSDDNEVILFDDNGEQHLPRADKLTLARLLINHIALRLQGRKL"
  },
  {
    "reference": "MDILCDGESSVNPTANSFIQINHERRFYRNVYGAGEINISHLCNLTVNSDNLTNLSCESNVSPPCCPSQKNWPALLTVIVIVLTIAGNILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSMLTILYGYEWPLPRKLCAIWIYLDVLFSTASIMHLCAISLDRYIAIRNPIHHSRFNSRTKAFAKIIAVWTISVGISMPVPVFGLQDDSKVFKKDIFKKDICLLADENFVLVGSFVAFFIPLTIMVVTYFLTIKSLQKEAMLCVNDIGPKTKFASFSFLPQSSLSSEKLFQRSLNRDTGTPGRRTMQSISNEQKASKVLGIVFFLFVVMWCPFFITNVMAVICKESCKEEVIGGLLNIFVWIGYLSSAVNPLVYTLFNKTYRSAFSRYIQCRYKEEKKPFQLILVNTIPALAYDSSQLQLAQMKSLKKEAKMMAKDYSAVMIGTHRLDGASKGSIGPGKEKVSGV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. A protein that can perform Gq/11-coupled serotonin receptor activity, serotonin binding, identical protein binding, 1-(4-iodo-2,5-dimethoxyphenyl)propan-2-amine binding in axon, cytoplasmic vesicle, caveola, G protein-coupled serotonin receptor complex, presynapse to support positive regulation of fat cell differentiation, positive regulation of phosphatidylinositol biosynthetic process, response to xenobiotic stimulus, positive regulation of kinase activity, glycolytic process.3. The designed protein must have at least one G-protein coupled receptors family 1 profile domain. The designed protein sequence is ",
    "response#1": "GNILVILAVSLEKKLQYATNYFLMSLAVADLLVGLLVMPIALLTILFDTAWPLPPGLCPVWLFLDVLFSTASIMHLCAISLDRYIAIKKPIQASQYNSRATALIKISVVWLISIGIAIPVPIRGIEDGDGNSTNITCVMEPNRFRVFILFGSLAAFFIPLAIMIVTYFLTIQVLHKKAYLINNKPSQRLTWNTVSTVFQRDGTPGSSPEKVAMLDGSRKDKTLPNTSEEMLMRRMSAVGKKSVQTITNEQRASKVLGIVFFLFLVMWCPFFITNITSVLCESCRPEVIQMLMEIFVWIGYVSSGVNPLVYTLRNKDVKAALKRLLLCK",
    "response#2": "WNLLVLVTILRVRAFHRVPHNLVASTAVSDVLVAALVMPLSLVSELSAGRRWQLGRSLCHVWISFDVLCCTASIWNVAAIALDRYWTITRHLQYTLRTRRRASALMIAITWALSALIALAPLLFGWGEAYDARLQRCQVSQEPSYAVFSTCGAFYVPLAVVLFVYWKIYKAAKFRFGRRRRAVVPLPATTQAKEAPQESETVFTARCRATVAFQTSGDSWREQKEKRAAMMVGILIGVFVLCWIPFFLTELVSPLCACSLPPIWKSIFLWLGYSNSFFNPLIYCCLNHRFRSGFRLAFRCCPWIQQEAEE",
    "response#3": "SNAFVIATVYQTRKLHTPANYLIASLAFTDLLVSILVMPISTLYTVTGKWTLGQVVCDIWLSSDITCCTASILHLCVIALDRYWAITDAVEYSTKRTPKRAAGMIALVWVFSICISMPPLFWRQAKAEEASNCAVNTDHVLYTVYSTVGAFYFPTLLLIALYGRIYVEARSRILKQTPKKAGKRLTRAQLITDSPGSSSSVTSINSKAPEGPSETGSPVYMNQVKVKVSDALLEKKKLTAARERKATKTLGIILGAFIVCWLPFFIISLVLPICKDACWFHMAIFDFFTWLGYLNSLINPIIYTIFNHDFRRAFKKILC"
  },
  {
    "reference": "MFKIGNLELQSRLLLGTGKFENEDVQTEAIKASETNVLTFAVRRMNLYDKNLPNPLANINLKDFITFPNTASAKTAEEAIRIAEIAKHAGVCDMIKVEVIGDDETLLPDPFETYEACKVLLERGYIVCPYISNDVVLAKRLEDLGVHAVMPLASPIGTGRGINNQLNLSYIIKNSNVPVIVDAGIGSPKDACHAMELGADGILLNTAVSGAKDPVKMAEAMKLGIHAGRLSYEAGRIPVKYTAQASSPTEGLGFL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a high specificity for 1-deoxy-D-xylulose 5-phosphate binding, with minimal off-target effects.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm that is critical for thiamine diphosphate biosynthetic process.3. A protein that can modulate the overall flux of metabolites through the Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "PSWTSVFGDEIVRIGAEREDIVAITAAMLHPVGLARFADRFPDRVWDVGIAEQHAAVSAAGLATGGLHPVVAVYATFLNRAFDQLLMDVALHRCGVTFVLDRAGVTGVDGASHNGMWDMSVLQVVPGLRIAAPRDADHVRAQLREAVAVDDAPTLIRFPKESVGEAGADGPTHQGAFDLSYLYPSYSEIFGQWLCDTASNDKSLMAITPAMREGSGMVTFSQQYPQQYFDVAIAEQHSVTYAAGLAISGLKPVVAIYSTFLQRGYDQLIHDVALQNLDVLFAIDRAGIVGADGPTHQGAFDLSYLRCIPNMVVMAPSNEQECLDMLTTGYQYKGPAAVRYPRGAGVGGKAEILKTGISNKLFIGYGAGVSRAIDTEALHNEDITVVDLRFVKPLDKNLLLELSKKYNDWYIFSDSQKQSGVGSAILEFLNDEKIHNVQLTSFEYNDKFIEHGDTKLVEQSLGLLPEGQDIAGGKAEILKTGISNKLFIGYGAGVSRAIDTEALHNEDITVVDLRFVKPLDKNLLLELSKKYNDWYIFSDSQKQSGVGSAILEFLNDEKIHNVQLTSFEYNDKFIEHGDTKLVEQSLGLLPEGQDVAGRILARL",
    "response#2": "PSWTSVFGDEIVRIGAEREDIVAITAAMLHPVGLARFADRFPDRVWDVGIAEQHAAVSAAGLATGGLHPVVAVYATFLNRAFDQLLMDVALHRCGVTFVLDRAGVTGVDGASHNGMWDMSVLQVVPGLRIAAPRDADHVRAQLREAVAVDDAPTLIRFPKEST",
    "response#3": "PSYSEIFGQWLCDTASNDKSLMAITPAMREGSGMVTFSQQYPQQYFDVAIAEQHSVTYAAGLAISGLKPVVAIYSTFLQRGYDQLIHDVALQNLDVLFAIDRAGIVGADGPTHQGAFDLSYLRCIPNMVVMAPSNEQECLDMLTTGYQYKGPAAVRYPRGAGVGH"
  },
  {
    "reference": "MTQQRVSSVFIETVPIVLASASPRRRSLLEQLGLLFKIVSVDSEPLPLSSEHPLEYVIRAAKVKAFAAAALEPRSVIIAADTVVIYTKDDVFEIIGKPQSGNDSFSMLSRFQGGKHQVITGCCIVWPLEENITSVQYEIFYDTASIQFGQWDKDILSSYVSTGESNDKAGAFSIQGIGAFLIDIIEGNYTTILGLPLPQLVKRLLKRKAIKVVLNKNSEETISSDFLTYSR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a specific function in nucleotide metabolic process and should be localized to cytoplasm in order to carry out this function efficiently.2. For general function, the protein need meet that May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids.3. The Proton acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "NVCHGSDSVESANKEIDLWFG",
    "response#2": "NVCHGSDSVESANKEIDLWFG",
    "response#3": "NVCHGSDSVESANKEIDLWFG"
  },
  {
    "reference": "MTTNESSYKADALIAEDIDAYLVQHQHKSLLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFATDKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSYLAHLIGIRNIVLAVNKMDLIDYDQAQYEAIVEDYRVFATSIGIEDFVAMPISGFKGDNITGPSPNTPWYKGPALIEHLETVELDVTTDQAKPFRMPVQWVNRPNLDFRGFSGLVATGTVKPGDAVRVLPSGKTSTVSRIVTMDGDLDQAVAGQSVTICLADEVDCSRGDVLSIADTPPQAADQFEATIVWMADEEMLPGRPYWLKLGTQTVTANIQAPKYQVNVNTMEHLAAKTLDLNAIGVANLSTDRQLVFEPYEQDRDLGGFILIDKLTNATVAAGMLHFSLRRAQNVHWQALDISRDAHSNLKNQKPAVLWFTGLSGAGKSTIANLVEKKLHRMNRHTFLLDGDNVRHGLNKDLGFTDADRVENIRRVGEVAKLMTDAGLIVITAFISPFRQERQMVRDMMKSGEFLEVHIDTPLAEAEARDVKGLYKKARSGELANFTGIDSPYEAPTNPEIRIDTTKMTPEQAADLIVEELLK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should consider the endogenous proteins and enzymes present in the sulfite from sulfate: step 2/3, hydrogen sulfide biosynthesis, Sulfur metabolism pathway to avoid interference with native metabolic processes.2. The protein design must be able to perform ATP binding, GTP binding, adenylylsulfate kinase activity in order to contribute to sulfate assimilation.3. The designed protein should have a binding site that is specific to the target GTP, ATP ligand.4. For general function, the protein need meet that With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway; APS synthesis involves the formation of a high-energy phosphoric- sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.5. The protein with the reaction adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate   + ADP + H(+), the Phosphoserine intermediate must be optimized to ensure efficient catalysis.6. The designed protein must have at least one Tr-type G domain. The designed protein sequence is ",
    "response#1": "MDLLRLSTAGSVDDGKSTLIGRLLYDTKSIFEDQLEAIERASRQRGEGYVNLALLTDGLRAEREQNITIDVAYRYFATPRRKFIIADTPGHVQYTRNMVTGASTAELAIVLVDARKGVLTQSRRHGFIASLLGIPHLVICVNKMDLVDYSEEVFEEIRDEYTGFASKLGVKDLTFLPISALRGDNVAAKSENTPWYDGSTLLHHLETVPVGSSFRMPVQWVNRPNLDFRGFSGLISDGEIAPGDTVRIVPSGKTSTVKSIVTMDGDKDEAVAGQSVTLTLADEVDCSRGDVIASAADPAEEVSDQFEATIVWMDEEALLPGRGYWMKLASQTVSATVAEPKYEININNVGGPGDHLAAKTLELNSIGVAEVTTDRPVVFEPYADNRALGGFILIDKITNRTVGAGMLHFALRRSHNIHEVADQFEATVVWMHDAPLLPGRPYLVKLGAQTVGATCATPKYKIDVNTREHLAARTLALNEIGVCNLSFDRPVAFDPYTQNRHTGGFIVIDRYTNDTVGAGEA",
    "response#2": "TMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALENDSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADCPGHEQYTRNMVTGASTADLAVILIDARKGVLVQTRRHSYLCHLLGIRNIVLAVNKMDLIDYDQARYDAIVAEYETFAKGIGIESFTAMPISGFKGDNIRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAILESDSKKVGTQGEEIDFALLVDGLAAEREQGITIDVAYRFFTTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILVDARKGVLVQTRRHSYLVHLLGIKHVVLAVNKMDLVDYDQATFDAIVDDYTAFAKEVGIEEFTAIPISGFKGDNITAKADGAPSSNTPWYSGPALINHLENVELKGLLRFITCGSVDDGKSTLIGRLLYDTKRLFDDQLAALESDSRRHGTQNGEIDYALLVDGLSAEREQGITIDVAYRFFSTEQRKFIVADCPGHEQYTRNMATGASTADVAVVLVDARKGLLTQTRRHSYIVSLLGIRHVLLAVNKMDLVGYDQAVFDDIVGGYRELAAQLGIEHVQAVPLSALRGDNLIERSPQTSWYGGPSVLEYLETVDPRRDFRLPVQMVTRPDLDFRGFAGTIASGVVRPGDKVVSLPSGVSSTVERIVTMDGDLAMAGPGRAITLTLTDEIDVSRGDLLVTEANRPQFEATIVWMDEAALKPGRGYWLKLGSQMVTATVQPPKHEVDVNSFEHLAAKTLELNSIGVAEIATDRPITFEPYESSRQLGGFILIDKFTNATVGAGMVRS",
    "response#3": "MDLLRLSTAGSVDDGKSTLIGRLLYDTKSIFEDQLEAIERASRQRGEGYVNLALLTDGLRAEREQNITIDVAYRYFATPRRKFIIADTPGHVQYTRNMVTGASTAELAIVLVDARKGVLTQSRRHGFIASLLGIPHLVICVNKMDLVDYSEEVFEEIRDEYTGFASKLGVKDLTFLPISALRGDNVAAKSENTPWYDGSTLLHHLETVPVGSSDLLRFITCGSVDDGKSTLIGRLLYESNQLFDDQLSQLEADSKKLGTQGGDLDFALLVDGLSAEREQGITIDVAYRFFATGRRKFIVADTPGHEQYTRNMITGASTADLAVILIDARKGVLTQTRRHSHLVALIGITRVVLAINKMDLVDYDGAVFERIDADYRAFAAELGLSEIVSIPLSALRGDNVIVPSTRMPWHDGPTLMQHLEVSDQFEATIVWMDEEALLPGRGYWMKLASQTVSATVAEPKYEININNVGGPGDHLAAKTLELNSIGVAEVTTDRPVVFEPYADNRALGGFILIDKITNRTVGAGEVVGHPSAREVSDQFEATIVWMDEEALLPGRGYWMKLASQTVSATVAEPKYEININNVGGPGDHLAAKTLELNSIGVAEVTTDRPVVFEPYADNRALGGFILIDKITNRTVGAGVVEWELDESVHRAANIHEVADQFEATVVWMHDAPLLPGRPYLVKLGAQTVGATCATPKYKIDVNTREHLAARTLALNEIGVCNLSFDRPVAFDPYTQNRHTGGFIVIDRYTNDTVGAGGTGASTCQLAIIGVNP"
  },
  {
    "reference": "MRRTILKSKIHRITITGADLHYEGSLTLDEAIMEAANLVPFEKIEIYNVNNGHRFSTYVIPGQRYGGECILNGAAARLGHAGDIIIIVSWADLDEEELKNFKVNLVYMDEENNIKEHKVTTVFSEEVKEIAERNKNLVRD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis, (R)-pantothenate biosynthesis, beta- alanine from L-aspartate: step 1/1 pathway to avoid interference with native metabolic processes.2. A protein that can perform aspartate 1-decarboxylase activity in cytoplasm to support alanine biosynthetic process, pantothenate biosynthetic process.3. The design of the protein must focus on the precise arrangement of amino acids in the Proton donor, Schiff-base intermediate with substrate; via pyruvic acid to enable effective catalysis of H(+) + L-aspartate = beta-alanine + CO2.4. The protein should be able to bind substrate ligand in a variety of conditions5. The presence of pyruvate is crucial for the protein to function optimally under specific conditions.6. For general function, the protein need meet that Catalyzes the pyruvoyl-dependent decarboxylation of aspartate to produce beta-alanine. The designed protein sequence is ",
    "response#1": "MKMLFQGDTLGASNLSKKIVFATGNENKLKEVRSILKDLSIELISLKDADIDVEIAEDGSTFAENAAKKARTIAEHTQNPVIADDSGLEIDYLNGAPGIYSARFAGEDNNYVKNNEKLLLELRNVPAPERTARFRCVIALIYPDGHVETAEGTMEGIIGEENRGNNGFGYDPVFYLPKLKKTMAELDEKEKNAISHRGKALKKLYEIMKKD",
    "response#2": "MGYSLDQIVKGVRVTGVEGRAVXVXVXDXSXXLPXEXFXEXRVXXSXVXGXGXFXYLXXMGXAXSXGXVIXTDXPMXMAXXAXFXXNXSXXTKLXGXHPSARIXXDAKIDESAIIGEXVQIDSHCRIGSXVXIXTXIXIGXIIGDDNXLHXGXXI",
    "response#3": "MNNEKKMTFAXETALLNXGXGXVXGXKXXEXPXPXEXFXEXXQXDXXFXEXDXXYRXAXGXMPXFXSNXSDXDX"
  },
  {
    "reference": "MKFLTTNFLKCSVKACDTSNDNFPLQYDGSKCQLVQDESIEFNPEFLLNIVDRVDWPAVLTVAAELGNNALPPTKPSFPSSIQELTDDDMAILNDLHTLLLQTSIAEGEMKCRNCGHIYYIKNGIPNLLLPPHLV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. helix-helix-strand-turn-strand-helix-helix-helix-helix-strand-turn-strand-strand is the desired arrangement for the engineered protein.2. The designed protein should be involved in ribosomal small subunit biogenesis, rRNA (guanine-N7)-methylation by localizing to nucleolus, tRNA (m2G10) methyltransferase complex and exhibiting protein heterodimerization activity. The designed protein sequence is ",
    "response#1": "WVRIGDVLLLPLRPELEPYKHRIAEVYAEVLGVKTVLRKGHIHGETRKPDYELLYGSDTVTVHVENGIKYKLDVAKIMFSPANVKERVRMAKVAKPDELVVDMFAGIGHLSLPIAVYGKAKVIAIEKDPYTFKFLVENIHLNKVEDRMSAYNMDNRDFPGENIADRILMGYVVRTHEFIPKALSIAKDGAIIHYHNTVPEKLMPREPFETFKRITKEYGYDVEKLNELKIKRYAPGVWHVVLDLRVFEAS",
    "response#2": "WVRIGDVLLLPLRPELEPYKHRIAEVYAEVLGVKTVLRKGHIHGETRKPDYELLYGSDTVTVHVENGIKYKLDVAKIMFSPANVKERVRMAKVAKPDELVVDMFAGIGHLSLPIAVYGKAKVIAIEKDPYTFKFLVENIHLNKVEDRMSAYNMDNRDFPGENIADRILMGYVVRTHEFIPKALSIAKDGAIIHYHNTVPEKLMPREPFETFKRITKEYGYDVEKLNELKIKRYAPGVWHVVLDLRVFGV",
    "response#3": "WVRIGDVLLLPLRPELEPYKHRIAEVYAEVLGVKTVLRKGHIHGETRKPDYELLYGSDTVTVHVENGIKYKLDVAKIMFSPANVKERVRMAKVAKPDELVVDMFAGIGHLSLPIAVYGKAKVIAIEKDPYTFKFLVENIHLNKVEDRMSAYNMDNRDFPGENIADRILMGYVVRTHEFIPKALSIAKDGAIIHYHNTVPEKLMPREPFETFKRITKEYGYDVEKLNELKIKRYAPGVWHVVLDLRVFE"
  },
  {
    "reference": "MHSATMVFKRAATPHPEKAEWSVDDVEALLGLPFMELVFRAAEIHRQFFDPTKVQLSTLVSIKTGGCPEDCGYCPQSVHHDTPVADQPMMTVDEVVAAARQAKANGAGRFCMGAAWRGPKDADLQKTLDMVREVKALGLETCATFGLLRDGQAEQLKDAGLDYYNHNLDTAPDKYGDIIQTREYEDRLDTLGKVRKAGLSVCCGGIVGMNETRRDRAGLIVQLANLDPQPESVPVNNLVQVVGTPLEGAERLDWTEFVRTIAVARITMPASYVRLSAGRREMDEATQALCFLAGANSIFYGDKLLTTGNPDVVADQSLMAKLNLQPL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit 4 iron, 4 sulfur cluster binding. The designed protein sequence is ",
    "response#1": "ELIFTDSAAAKVKDLLAEEGNPELKLRVFVQGGGCSGFQYGFTFDEAVNEDDTVLDKNGVQLLVDPMSFQYLVGAEIDYKEDLEGAQFVIRNPNASTTCGCG",
    "response#2": "LIFTDSAAAKVKDLLAEEGNPELKLRVFVQGGGCSGFQYGFTFDEAVNEDDTVLDKNGVQLLVDPMSFQYLVGAEIDYKEDLEGAQFVIRNPNASTTCGCGV",
    "response#3": "FVIRNPNASTTCGCGSSC"
  },
  {
    "reference": "MVKLVLASSNAGKLAELRELLANTGLDVQAQSAFGVGDAVESGASFIENALIKARHASAATGLPALGDDSGLLVDALDGAPGLYSARYAGDHGDVPANNALLLQRMAGVPAPERRAHFYCVLALLRSPSDPQPIIAEGIWPGFILEAPRGERGFGYDPLFFDPAQQLGAAEMAPELKNRISHRGLALASLRDRLRDAWR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be optimized for metal ion binding, XTP diphosphatase activity, dITP diphosphatase activity, ribonucleoside triphosphate phosphatase activity activity in order to improve nucleotide metabolic process, purine nucleoside triphosphate catabolic process.2. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+).3. The substrate binding site should be stable and able to withstand changes in environmental conditions.4. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.5. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.. The designed protein sequence is ",
    "response#1": "MELVFATHNRHKTDEVRALLGARFAVLGLADLGCADELPETAATIEGNARLKAEALARRLGLPALADDSGIEVEALGGAPGVRSARYAQDGTPDADNNAKLLAELAGVPWERRTARFHTTLVLVRGDEERVAVGTCEGRIATAPRGDRGFGYDPVFVPSGSDRTFAEMSLEEKARYSHRAEAVRALLPFLAR",
    "response#2": "MKLVFATNNQHKLSELRAILPDGIELLSLDDIGCTEDIPETADTLEGNALLKANYVTENYGYDCFADDTGLEVEALDGAPGVYSARYAGPQKDAEANMNKLLEVLKGTDNRKAQFKTVIALNLNGKQHLFEGIVNGTIVREQKGEKGFGYDPIFVPEGFDKTFAELGEEIKNKISHRAIAVRKLVEYLKSL",
    "response#3": "MFLLASGNAGKIRELATLLAPLGIDVKVQSQFDVPECPEPHETGTTFAENALIKARAAAELSGLPALADDSGLCVDALRGAPGVLSARYSGEPKSDARNNAKLLAELADAPEDRRAAHYVCVLVLVRSADDPAPIIAEGRWHGSITRAPRGAHGFGYDPIFFVPELDATAGELDPALKNAISHRGQALRAFLDRLQDD"
  },
  {
    "reference": "MFRGASSLSLDDKGRFAVPTKYRDDLLSEDQGTVICTVALNEPCLWLYPLAQWQEIESRLAKISNMNPRARRMQRMLLGNATEYQLDKNGRILLAPSLRAHADLGKKIMLVGLMNKFEIWDEARWHQQMRQDTELERLGDFEPHPDLDNFTL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must have at least one SpoVT-AbrB domain.2. A protein that localizes to nucleoid and can perform DNA-binding transcription factor activity, DNA binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "FVSSAHEVDLDVHGRVLVPPPLREWAGLQKDVVWVGMGRTIHLYDRAAYDEQMSACG",
    "response#2": "MK",
    "response#3": "MFRGINAITIDTKGRLAIPTRYRSALGAEDKIPLVVTIDTEETCLLLYTAAQWQIIEDNLQKLPSFNAAARRIQF"
  },
  {
    "reference": "MKTIFEALKWASSYLTEAGREENAAELLLLYDTGMERSKLLASLQEPIGEDELYRFKRHVEMHKEGVPVQYIIGKEFFYGREFMVNDDVLIPRPETEEVVFHLLEKYRSVFSEDGKLEVVDVGTGSGAIAVTLALENQSFSVSAVDISKEALQVASANAEKLGANVRFYQGDLLEPFIKAGKKADIIVSNPPYISEEEMADLSEIVRFHEPLHALTDGGDGLKFYKRFMEDIPLVMKDKVFVVFEIGWKQGAAVKDLILKAFKGAEVEVLKDINGKDRTICALIHKNK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The substrate binding site should be stable and able to withstand changes in environmental conditions.2. The protein must exhibit nucleic acid binding, protein-(glutamine-N5) methyltransferase activity.3. For general function, the protein need meet that Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif. The designed protein sequence is ",
    "response#1": "LEELRRVEKPRVLDVGTGTGALALGLKAAIPQAEVTATDLSPEALSLARENAALSGLDVKFVEGSLLAGLSGPFNLIVSNPPYY",
    "response#2": "LSDRPSPTVLDIGTGSGAIAVAIAKSLPKTQVTAVDISLTALDIAKWNVENLKLSDRVTLLQSDLFDGLEPDQTFDVICSNPPYISQSV",
    "response#3": "QMHKEKTTFYDVCCGSGCIGLAVRKHCPHVRVTLSDISPEALAIAESNARSNALAVDFLLGDLFDPFSFPADVLVCNPPYLY"
  },
  {
    "reference": "MLRLLLALNLFPSIQVTGNKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKPFWVLVVVGGVLACYSLLVTVAFIIFWVRSKRSRLLHSDYMNMTPRRPGPTRKHYQPYAPPRDFAAYRS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The intended protein structure should align with strand-strand-strand-strand-strand-strand-strand-strand-helix-strand-strand-strand criteria. The designed protein sequence is ",
    "response#1": "QKRTPLMQVPVLVDLNGNHLWVNCEQQYSSKTYQAPFCHSTQCSRANTHQCLSCPAASRPGCHKNTCGLMSTNPITQQTGLGELGEDVLAIHATQGSTQQLGPLVTVPQFLFSCAPSFLVQKGLPRNTQGVAGLGHAPISLPNQLASHFGLQRQFTTCLSRYPTSKGAIIFGDAPNNMRQFQNQDIFHDLAFTPLTITLQGEYNVRVNSIRINQHSVFPLNKISSTIVGSTSGGTMISTSTPHMVLQQSVYQAFTQVFAQQLPKQAQVKSVAPFGLCFNSNKINAYPSVDLVMDKPNGPVWRISGEDLMVQAQPGVTCLGVMNGGMQPRAEITLGARQLEENLVVFDLARSRVGFSTV",
    "response#2": "QKRTPLMQVPVLVDLNGNHLWVNCEQQYSSKTYQAPFCHSTQCSRANTHQCLSCPAASRPGCHKNTCGLMSTNPITQQTGLGELGEDVLAIHATQGSTQQLGPLVTVPQFLFSCAPSFLVQKGLPRNTQGVAGLGHAPISLPNQLASHFGLQRQFTTCLSRYPTSKGAIIFGDAPNNMRQFQNQDIFHDLAFTPLTITLQGEYNVRVNSIRINQHSVFPLNKISSTIVGSTSGGTMISTSTPHMVLQQSVYQAFTQVFAQQLPKQAQVKSVAPFGLCFNSNKINAYPSVDLVMDKPNGPVWRISGEDLMVQAQPGVTCLGVMNGGMQPRAEITLGARQLEENLVVFDLARSRVGFSTV",
    "response#3": "QKRTPLMQVPVLVDLNGNHLWVNCEQQYSSKTYQAPFCHSTQCSRANTHQCLSCPAASRPGCHKNTCGLMSTNPITQQTGLGELGEDVLAIHATQGSTQQLGPLVTVPQFLFSCAPSFLVQKGLPRNTQGVAGLGHAPISLPNQLASHFGLQRQFTTCLSRYPTSKGAIIFGDAPNNMRQFQNQDIFHDLAFTPLTITLQGEYNVRVNSIRINQHSVFPLNKISSTIVGSTSGGTMISTSTPHMVLQQSVYQAFTQVFAQQLPKQAQVKSVAPFGLCFNSNKINAYPSVDLVMDKPNGPVWRISGEDLMVQAQPGVTCLGVMNGGMQPRAEITLGARQLEENLVVFDLARSRVGFSTN"
  },
  {
    "reference": "MSPDESLETLLGTAARLRPDLPVEVVAGSVGAARVGRLVDDSRQVIPGAGFVARPGTVSDGRLHILDAIERGATLVIAEPSAWDGIDPGSMKGIVALACPGPLELGTTLANIRRGDPRSGLRIAGITGPNGKTTVASLVRQVLEAGVARCGSIGTIEIHDGVQAVPARVTTPGRLEIVEILGDMVDNGCELLAMEVSSHALDQGRVEDLDFAVGVFTNLSGDHLDYHGTMEAYAAAKARLFRSLGEDAVAIVNLDDAASASMLESCVAGTIGVTRLDVEDRVAPAKVDRVVGIEILRNDADGMRLRWTGILDRPLEIDVPLIGVHNAFNATASLLVAIEMGAEVSDAVEAIRGISAPRGRLEPVHEEGDSIRVYIDYAHTDDAIRTVLSAVRPFVPEDAALVAVIGAGGDRDRTKRPRMMRAALENADRVVVTSDNPRTEDPAAIVAEVVGGATAGEASRFRQEVDRRRGIARAIEEASPGDVIVIAGKGHEAHQIVGTERRPFDDRAVAREALSDRREGGR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must have at least one Mur ligase central domain.2. A protein that can perform UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, magnesium ion binding in cytoplasm to support peptidoglycan biosynthetic process, cell cycle.3. The designed protein should interact with enzymes and substrates within the Cell wall biogenesis pathway to enhance or regulate its activity.4. The designed protein should have a binding site that is specific to the target meso-2,6-diaminoheptanedioate, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate ligand.5. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "VTGTNGKTTTTYLIDAIYTHAGIRTGTLGTTGLKVGDSLQALPNTTPESLTLQQAFRDMVDQQVTAGIMEVSSHALVTGRTRGCQFDVAIFTNLTQDHLDYHQTLTAYQDAKRLLFASLPAKTKTGKPTFAILNVDDKASAGFRDVTQGLVVTYGLENEADFSASQLVLKSKGTTFNLKTPDGFFAMTLPLAGKFNVYNALAAAAAALVTGVPLETITGTNGKTTTTQWLAQLLQLLGTPAASVGTLGFGRVGEEVVATGLTTPDAVSLQRILRQMLDQGTAAVVMEASSHSLDQRRLAGVSVDVAVFTNIGRDHLDYHGDMNSYVAAKLKLMDFPSLQSAVVNADDPVAAEFIARAAGHCRVLTCSLQGKADFMVSQVRYHAGGIDAELVTPEGEFDLQLAIWGEFNLSNLLVVIAACRALGVGLEAIERGIQAAPAVPGRMELIAIEQGPSVVVDFAHTPDALEQVLKSLKAHVDGKLYCVFGCGGDRDKGKRPQMGRVAGSLADVVVLTSDNPRSENPDDIIEDIKRGLKSADKTIVEPSRAQAIRLAIESASKGDVVLVAGKGHEDYQILGTEKIHFDDREAAREALERRGLL",
    "response#2": "MSLSGINQIEADSRKVNKGSLFVCIEGFTVDGHDFVHQAVENGASAILAGKDVNASVPVIRVSDTSKSMAIVASKFYGFPSEKLTLVGVTGTNGKTTISYLIESIFQTSQQQTGLVGTIQMKIGEEEYEVKNTTPDALFLQKNFAKMVDQKVDTAVMEVSSHALDLGRVHGCDFDVAVFTNLSQDHLDFHKDMDDYLRAKSLLFSQLGNAYESNPKYAVLNQDDPHYASLMKSTAQEVITYGIDSEADITAKQLHLSAQGTEFILKTPDDEIEMKSSLIGRFSVYNMLAAASAAIAMGEDEQKILAGLSKFKAVAGRLEILAESPTVLRDYAHTPDALERALDAVRPFTRRRLIAVFGCGGDRDRGKRAVMGRIAAERADVVIVTSDNPRTEDPERILDDIVGGMPRGPHERIEDRREAIARALSMAVEEDLIVLAGKGHENYQEINGVRSHFSDQEVVREFLQKLK",
    "response#3": "ITGTNGKTTTTQWLAQLLQLLGTPAASVGTLGFGRVGEEVVATGLTTPDAVSLQRILRQMLDQGTAAVVMEASSHSLDQRRLAGVSVDVAVFTNIGRDHLDYHGDMNSYVAAKLKLMDFPSLQSAVVNADDPVAAEFIARAAGHCRVLTCSLQGKADFMVSQVRYHAGGIDAELVTPEGEFDLQLAIWGEFNLSNLLVVIAACHEFGVDPRDVVTGLTTFTGVKGRLEILAESPTVLRDYAHTPDALERALDAVRPFTRRRLIAVFGCGGDRDRGKRAVMGRIAAERADVVIVTSDNPRTEDPERILDDIVGGMPRGPHERIEDRREAIARALSMAVEEDLIVLAGKGHEDYQILGTEKIPFSDQAEARDALEARLAT"
  },
  {
    "reference": "MSKLIYIMGPSGSGKDSLMAEARLRLAAEAPVVFAHRYITRPADAGGENHVALSRAEFQLRLSRGLFALSWESHGFAYGIGREIDIWMESGLSVVVNGSRGALSAALQAYPELLPVLIDVPEHILRERLGARGREDAGEIEARLVRARMAVVEAPELVRFDNSGPLAERGQALAGLILETTISGRKS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should take into account the physiological conditions of the Metabolic intermediate biosynthesis pathway.2. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.3. The designed protein should have ATP binding to facilitate 5-phosphoribose 1-diphosphate biosynthetic process, organic phosphonate metabolic process.4. The protein should have a fast turnover rate for alpha-D-ribose 1,5-bisphosphate + ATP = 5-phospho-alpha-D-   ribose 1-diphosphate + ADP. The designed protein sequence is ",
    "response#1": "SPILLEDMFELNLENPIVVSPDIGGVIRARAIAKLLNDADMAIIDKRRPRANISQVMHVIGDVLGRECVLVDDIIDTGSTLCKAAEALKEHGAKRVFAYATHPVFSGNAMDNLRKSMIDEVVACDA",
    "response#2": "ASPVFFQYLASKRSNNLVVFSPDVGGMKMAAAYADVLGASLGLVAKKRTSATKVEAINIVGEVAGCDVLLVDDITETAGTLTAAAKILKDHGAKSIRAAVSHCVLNEMAYERLKTGLIDELITTNQA",
    "response#3": "MIVIVGPSGAGKDTLMDYAAAQLSGRPGFHFTRRVITRSCDAGGENHDAVSMHEFNQLEDAGAFAVSWQAHGLKYGIPAAVYRHLEAGDVVIANGSRSALPHFGTAFSRLKVVNIVARPDVLARRLEQRGRESRDDILRRLERSSLAVAGDFDVTTVDNSGAIEDAGKTIMQVLDG"
  },
  {
    "reference": "MNRPIPARLDSRAPDFTARLSALLAYEPAQDEAIERTVAQIVADVRKRGDEALLEYTRRFDRLEAACARALELPGDELRGALDSLAPADRDALQAAAARIRGYHERQLAHSWSYVDDDGTRLGQRISPIDRVGLYVPGGKAAYPSSVLMNALPARVAGVRELVMVVPTPDGVRNPMVLAAACIAGVDRVFTIGGAQAVAALAWGTETVPAVDKIVGPGNAYVAEAKRRVFGTVGIDMIAGPSEILVLCDGSTDPDWIAADLFSQAEHDEMAQAILLTPDAAFADRVAESIARLLPTMPRREIIERSLADRGAIVVVRSMEEACEIATTIAAEHVEISARDAGRWVDRIRHAGAIFLGPWSSEAIGDYCAGPNHVLPTMRTARFSSPLGVYDFQKRTSLIEVSGDGARTLGAIAATLARAEGLEAHARSAQLRVEAPRVRRESAAPERFDPIRPEIVEMSGYQVLAASGMIKLDAMENPYRMPPELRRALGEGLAEVAINRYPAPGHDELAQAIRERFAVPADAGLVLGNGSDELITMLAVAVARPGAVHLAPQPSFVMYELSARLAGSRFVGVALRADFSLDIDAMLSAIAAHRPAVVWLAWPNNPTGNAFAREDVEAVLAAAPGLVVLDEAYQPFAQDTWMQRLRDHERLVVLRTVSKLGLAGLRLGYLAAATRWTEQLQKVRPPYNVNVLTDAAARFALGHLDVLDAQAARIRAGRELLAQRLRELPGVEVFPSLANFVLVRVADSERTERGLRSRGLLIKDLGRM",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit zinc ion binding.2. The protein with the reaction H2O + L-histidinol + 2 NAD(+) = 3 H(+) + L-histidine + 2 NADH, the Proton acceptor must be optimized to ensure efficient catalysis.3. A protein should be able to manipulate the activity of enzymes in the L-histidine biosynthesis, L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9 pathway, either by activating or inhibiting their function.4. The protein must be engineered to have a high affinity for pyridoxal 5'-phosphate in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "IMFALPNKGRISEPVMKVLEKAGLKITVKGRSLFANTVDDNIKVMFARARDIPEFVADGVADIGVTGYDLVLERNVEDKVDFLLDFGFGFAKLVLAAPESSNINSIDDIKEGMRVATEFPNLTKKYFEKLNKKVEIIELSGATEIAPFIGIADLISDLTSTGTTLRLNRLKVIDEIVSSTTRLIEA",
    "response#2": "LNKKTFNKYPEPQSSKLINLYSKYANLNYKNILVTRGADEGIDIIMRTFCNPVKEKILFCPPTYDMYRVNAEILNIKYIMINSLNNWELNVNFIKKNLYNIKIIFICHPNNPTGNCINKNNIIKLLKLVKNKIIIVIDEAYIEFCINKTLVNLIDKYHNLIILRTLSKAFGLAGLRCGFILTNSKIINILQKVIAPYPIPDPVIDIAIQALYPKNLIIMKNNVKKIIDNKKWLINNLKHYKIIKKIFYSETNFVLIKFFNSHYVFKELYKNKIIVP",
    "response#3": "IMFALPNKGRISEPVMKVLEKAGLKITVKGRSLFANTVDDNIKVMFARARDIPEFVADGVADIGVTGYDLVLERNVEDKVDFLLDFGFGFAKLVLAAPESSNINSIDDIKEGMRVATEFPNLTKKYFEKLNKKVEIIELSGATEIAPFIGIADLISDLTSTGTTLRLNRLKVIDEIVSSTTRLIEA"
  },
  {
    "reference": "MAKITKEIVFGNHNLILETGEVARQADGAVMASMNGTQVLVTVVWKKDGGESNDFFPLTVNYQEKFYAIGKIPGGFNKREGRPSDNETLISRLIDRPIRPLFPDNFFNEVQIIATVLSLNPEVSPDIIAMIGASAALSISGVPFNGPIGAARVGYKDGVYLLNPSRKEQEESKLDLVIAGTKDAILMVESEAQELSEDIMRGAMLYGHEMMKNVIKSIEELAREVGKSKPEWKAPEIDTVLKARINDVARNEVEAAYLIKDKQQRYQRLDELREQTISALLAENDELNADVIANMFGELERSIVRNRILDGEPRIDGRDHRTVRPISIRTKFLERTHGSCLFTRGETQAIVVATLGNERDAQILDGISGESRDRFMLHYNFPPYSVGETGQVGSPKRREIGHGRLAKRALMAVLPDANEFPYVLRIVSEITESNGSSSMATVCGTSLALMDAGVPLKAPVAGVAMGLIKEGDRYAVLTDILGDEDHLGDMDFKVAGTEKGITALQMDIKISGITNEIMEQALEQALEGRTHILGVMNNALAEHRTELSQHAPRITTMKVAEDKIRTIIGKGGATIKGLIESTGVSIDIDDSGVIQLFSPDKMALEEAQKQIKALIAEIEVGQTYQGKVSKIVDFGAFINLLPGKDGLLHISQICADRTQKVEEVLQEGQEIEVFVAGIDKQGRVKLEWKDKPQAEAKEVEDAPVSATFLTMEEQSEEINSGNKISEEEE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design must be able to perform polyribonucleotide nucleotidyltransferase activity, magnesium ion binding, RNA binding in order to contribute to mRNA catabolic process.2. We are looking for a protein with broad substrate specificity for phosphate + RNA(n+1) = a ribonucleoside 5'-diphosphate +   RNA(n).3. The protein contains novel S1 motif domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "ELTETVRVLCETRLKELAYTPLKKEERADQTAAIYHEIIESTIEHFKATCSSEEFSTDPAKALCINPHIIDEQIHMVEKRVMRHMILDDAKRLDGL",
    "response#2": "LEKEMLGLIEGELRPAYAIADKQQRQNAVAAAKAKVIAHYFPEGQEPKYDKLRIAAVFKHLEAKIVRWNILDTGKRIDGRL",
    "response#3": "LEKEMLGLIEGELRPAYAIADKQQRQNAVAAAKAKVIAHYFPEGQEPKYDKLRIAAVFKHLEAKIVRWNILDTGKRIDGRR"
  },
  {
    "reference": "MQLSPQEKDKLLIFTAALLAERRKNRGIKLNYPEAIAYISAAILEGAREGRSVAELMSYGTTLLTREEVMEGIPEMITEVQIEATFPDGTKLVTVHNPIH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have nickel cation binding, urease activity within cytoplasm to regulate urea catabolic process.2. The protein design should take into account the physiological conditions of the Nitrogen metabolism pathway.3. The desired protein should be able to perform 2 H(+) + H2O + urea = CO2 + 2 NH4(+) The designed protein sequence is ",
    "response#1": "GGIDTHVHFITPAIAYSALENGTTTIIGGGTGPADGTNSATSTPGAWNIHQMLRAAEGMPVNMGIQGKAGGAIMDTTAEQIEAGAMALKVHEDWGATLSCIDHALETADKYDVQVSLHSDTLNETGFVEDTIKSIGGRCIHSYHTEGAGGGHAPDLMKVASKNNIIPSSTSPTNPYTVDILPEHLDMLMVCHHLDPKIPEDVRFADSRIRKQTIAAEDVLQDMGALSIMSSDTMAMGRIGEVIMRSWQLADKMKKQRGPLEGDSEYIDNNRIKRYISKYTINPAIAEGISDYVGSIEEGKYADLVLWEPAMFGAKPKMILKSGMIAYGVMGDSNASIPTTQPRTMRELFGLTGKSRQHVNMTFVSTYAYEHNIKEELGLERNVLPVHNVRTVTKKDMKFNSETPKIEIDPLTYDVTVDGKLITCDPARELPLAQRYLYGFGDVI",
    "response#2": "GGIDTHVHFITPAIAYSALENGTTTIIGGGTGPADGTNSATSTPGAWNIHQMLRAAEGMPVNMGIQGKAGGAIMDTTAEQIEAGAMALKVHEDWGATLSCIDHALETADKYDVQVSLHSDTLNETGFVEDTIKSIGGRCIHSYHTEGAGGGHAPDLMKVASKNNIIPSSTSPTNPYTVDILPEHLDMLMVCHHLDPKIPEDVRFADSRIRKQTIAAEDVLQDMGALSIMSSDTMAMGRIGEVIMRSWQLADKMKKQRGPLEGDSEYIDNNRIKRYISKYTINPAIAEGISDYVGSIEEGKYADLVLWEPAMFGAKPKMILKSGMIAYGVMGDSNASIPTTQPRTMRELFGLTGKSRQHVNMTFVSTYAYEHNIKEELGLERNVLPVHNVRTVTKKDMKFNSETPKIEIDPLTYDVTVDGKLITCDPARELPLAQRYLYGAAE",
    "response#3": "GGIDSHIHFISPDQIETALVSGITTMIGGGTGPSESTKATTITPGAWNIHNMLRSFEQFPMNFGLLGKGHGSSVPAMAEQITAGAIGLKIHEDWGATPSSINTSLQVADEYDVQVAIHTDTLNEAGFVEDTRAAIAGRVIHTFHTEGAGGGHAPDIIELAQDPNILPASTNPTLPYTRNTLEEHLDMLMVCHHLNPGIPEDVAFADSRIRKETIAAEDVLQDMGIFSMTSSDSQAMGRVGEVVLRTWQVADSMKRQRGPLPEDEGTGGDNHRIRRYIAKYTINPATAHGISHAVGSVEEGKFADLVLWEPRFFGVKPDLILKGGQMVHGVMGDPNASIPTPQPRWYRRAFGAYGTAVAASSITFLSRAAIRAGVPETLGLRKVVRECRDIRTLTKADMKLNGETPPLRVDPQTYEVRVDGTPVTCEPQHVLPMAQRYFLSAADYCSAGS"
  },
  {
    "reference": "MDLGKPMKSVLVVALLVIFQVCLCQDEVTDDYIGDNTTVDYTLFESLCSKKDVRNFKAWFLPIMYSIICFVGLLGNGLVVLTYIYFKRLKTMTDTYLLNLAVADILFLLTLPFWAYSAAKSWVFGVHFCKLIFAIYKMSFFSGMLLLLCISIDRYVAIVQAVSAHRHRARVLLISKLSCVGIWILATVLSIPELLYSDLQRSSSEQAMRCSLITEHVEAFITIQVAQMVIGFLVPLLAMSFCYLVIIRTLLQARNFERNKAIKVIIAVVVVFIVFQLPYNGVVLAQTVANFNITSSTCELSKQLNIAYDVTYSLACVRCCVNPFLYAFIGVKFRNDLFKLFKDLGCLSQEQLRQWSSCRHIRRSSMSVEAETTTTFSP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Incorporate a signal peptide in the protein design.2. The Extracellular, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.3. For general function, the protein need meet that Receptor for the MIP-3-beta chemokine; Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.4. The target protein must exhibit helix-helix-helix-helix-helix-helix-helix-helix-strand-strand-helix-helix as its primary conformation.5. The ideal protein would be one that plays a critical role in homeostasis of number of cells, positive regulation of immunological synapse formation, positive regulation of filopodium assembly, negative regulation of interleukin-12 production, response to lipopolysaccharide through chemokine (C-C motif) ligand 19 binding, C-C chemokine binding within external side of plasma membrane, mitochondrion.6. The protein should contain a Helical; Name=4, Helical; Name=5, Helical; Name=2, Helical; Name=1, Helical; Name=6, Helical; Name=7, Helical; Name=3 membrane-spanning region. The designed protein sequence is ",
    "response#1": "GNMLVVLILINCKRPKSMTDIYLLNLAISDLFFLFTVPFWAHYAAGQWDFGNTMCQFLTGLYFIGFFSGIFFIILLTIDRYLAIVHAVFALKARTVTFGVVTSVTTWVVAVFASLPGIIFTRSQKEGYHYTCSPHFPFGQYQFWKNFETLKMVILGLVLPLLVMVICYSGILKTLLRCRNEKKRHRAVRLIFAIMIVYFIFWAPYNIVLLLNTYQEFFGLNNCSSSNRLDQAMQVTETLGMTHCCVNPIIYSYKDENFKRVAEIRAIGPTLGQDAIQAGIRSALIGTLAIFLLIFAYYGPHLGLVASLGLLYTSALILGLLSGLGATLTLPGIAGLVLTLGAAVDGNVLSFERIKEELRAGKKLRQAIPEGFRHSTLTIMDVNIAHLLAAAALYQYATGPVRGFAVILAIGVVASVFSNLVFSRHLLEREQKAKEKKQQQSHRPPPPPPPSLLPSETSDHTECGLGLSSLGLSSPGWL",
    "response#2": "LNTVALWVFVHIPSNSTFIVYLKNTLVADLIMALMLPFKILSDSHLAPWQLRGFVCTLSSVVFYETMYVGIMMLGLIAFDRFLKIIMPFRKTFVKKTAFAKTVSISVWSLMFFISLPNMILNKEATPSSVKKCASLKSPLGLWWHQVVSHTCQFIFWAVFILMLLFYAVITKKVYNSYRKFRSKDSRHKRLEVKVFIVMAVFFVCFAPLHFVRIPYTYSQTTNKTDCRLENQLFIAKEATLFLATTNICMDPLIYAFRSQELRQAFRRFTPEGAKKFEEVTRQNIGKRLAIVLDGRVYTAPVIRQAITGGQAVIEGLSSVEEASEIALVLRSGSLPVPLKVAEIRAIGPTLGQDAIQAGIRSALIGTLAIFLLIFAYYGPHLGLVASLGLLYTSALILGLLSGLGATLTLPGIAGLVLTLGAAVDGNVLSFERIKEELRAGKKLRQAIPEGFRHSTLTIMDVNIAHLLAAAALYQYATGPVRGFAVILAIGVVASVFSP",
    "response#3": "GNGLVIYVAGFRMTHTVTTICYLNLALSDFSYMTSLPFQITSIVMNGEWLFGWFLCKFVHMIINVNLFLSIFLITFIAMDRCICVLHPVWAQNHRTVNLARKVILGSWILVLMLIFPHFFFLTTVKDESGKVHCICNFESWAATPEEQVNMSMTVSLISVTLSFIVGFSIPMIFIVICYGLMAAKIGRRGLVNSSRPLRVLTAVAFSFFVCWFPFQLIFLLGNIGNKETQNNIDAWVNPASTLASFNSCLNPILYCWLNENFRRSFKHVAEIRAIGPTLGQDAIQAGIRSALIGTLAIFLLIFAYYGPHLGLVASLGLLYTSALILGLLSGLGATLTLPGIAGLVLTLGAAVDGNVLSFERIKEELRAGKKLRQAIPEGFRHSTLTIMDVNIAHLLAAAALYQYATGPVRGFAVILAIGVVASVFSNLVFSRHLLERQRRQRTWGFTPEGAKKFEEVTRQNIGKRLAIVLDGRVYTAPVIRQAITGGQAVIEGLSSVEEASEIALVLRSGSLPVPLKVAEIRAIGPTLGQDAIQAGIRSALIGTLAIFLLIFAYYGPHLGLVASLGLLYTSALILGLLSGLGATLTLPGIAGLVLTLGAAVDGNVLSFERIKEELRAGKKLRQAIPEGFRHSTLTIMDVNIAHLLAAAALYQYATGPVRGFAVILAIGVVASVFSKATVLASETVGPAIGEELRRNAVMAVLVGLGLILLYVAFRFDWTFGVASILAVAHDVAIVAGMYSLLGLEFSIPTIAALLTIVGYSINDSIVVSDRIRENQKLLRHLPYAELVNRSINQTLSRTVMTSLTTLLPILALLFLGGSVLRDFALAIFVGIFVGTYSSIYVVSALVVAWK"
  },
  {
    "reference": "MYAVIKTGGKQYRVAAGEKIKVEQIAADVGQEIVFNEVLAVGNGAELKVGTPLVSGATVSVTVLAHGKHDKVSIFKMRRRKHYQKRQGHRQQFTELLVNTIAG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that can perform rRNA binding in ribosome, ribonucleoprotein complex, cytoplasm to support translation. The designed protein sequence is ",
    "response#1": "MRPMTEEESKTFFEKLIKFIGKNVTLLIQRKDEPYCFRIQKNKVYYVSEELMKRSQNIPREGLCSLGTCFGRFTKTGKFKLNL",
    "response#2": "RPMTEEESKTFFEKLIKFIGKNVTLLIQRKDEPYCFRIQKNKVYYVSEELMKRSQNIPREGLCSLGTCFGRFTKTGKFKLNITCLDC",
    "response#3": "RPMTEEESKTFFEKLIKFIGKNVTLLIQRKDEPYCFRIQKNKVYYVSEELMKRSQNIPREGLCSLGTCFGRFTKTGKFKLNITCLDD"
  },
  {
    "reference": "MAPSARRSIRKKAAVSGTGLHTGVRTEATFLPAPAGQGIVFRRVDLAGKPEVPARLSEVEAVERRTAIGKGETTIHTVEHLLAAVAAHEIDDLTVELTGPEPPILDGSFQPYFEALAHAGPTETSGEPVILTVPAPFTVTDGDSTYVVAPAKELRLTVTIEWPHPLIGRQSGSYTVTAQAFAAELAPARTFGFVSEVESLKARGLIKGASQANAILLDERGLANGAQLRWPDEFLRHKAADIVGDLALTGARIRAHVVAERPSHSGNIAVARALARASRTSGAAPKMDIGRIMDVLPHRYPFLLVDRIIEVEGQQRIVGIKNVTINEPFFQGHFPGHPIMPGVLIIEAMAQVGGMLLMGYFEGQNVEDKVVYFMSLDNVKFRRPVVPGDQIRFELEMVQFRGKTCKMKGVGYVDGQVVAEADMMATVVDR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be involved in fatty acid biosynthetic process by localizing to cytoplasm and exhibiting UDP-3-O-acyl-N-acetylglucosamine deacetylase activity.2. The protein design should take into account the physiological conditions of the lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 2/6, Glycolipid biosynthesis pathway.3. The Proton donor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "MMHGVSPAKMILVAAGRGDRFAAAGADAPKQYRPLAGKPVLRWSVEAFLAAGFADVVVVSPAGHTELAAELAGLGVRRVEGGATRADSVRAGLAALPARAGADDA",
    "response#2": "MPVLHATENGRK",
    "response#3": "MCWLWHIGNH"
  },
  {
    "reference": "MPGTLYVVSAPSGAGKTSLVNALVRQDEAVSLSVSHTTRPPRPGEEDGVNYHFVDRDRFQALVAQGDFLEHAEVFGNHYGTSRSAVQALLDQGQDVILEIDWQGARQVRERMPGCLSVFILPPSREELRRRLTQRGQDEPEVIDRRMAEAVSEMSHYAEYDYLLVNDDFDRTLADLQAIFTANRHRLERQEPLLAETLRDLLG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that localizes to cytoplasm and can perform ATP binding, guanylate kinase activity with high specificity and efficiency.2. The ATP binding site should be located in a region of the protein that is accessible to the ligand.3. The protein should have a low Km value for ATP + GMP = ADP + GDP.4. The designed protein should contain one or more Guanylate kinase-like domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MSKLIILSGPSGVGKGTIESLLLKNKNLLIKLAISATTREKRRDEINGVNYFFLTVQEFKEKIENDEFIEWSCHFNNYYGTLKSQIKFIQSQNFIPLLEIDTTGAKNIIENYKNKGELSQLLTIFILPPSIESLKNRIQKRLTETNIQINQRLEKAKAEIKIKNLFKFQVVNDNLEECVAQIEKIISKEV",
    "response#2": "RGVMLVLSSPSGAGKSTIARSLLENDHEFELSVSVTTRPRRPSEIEGVHYHFKTQRDFEMMRDGGDLLEWAEVHGNCYGTPRGPVERAIAGGRDMLFDIDWQGAAQLREKMPDDIVSVFILPPTMKELLARLTRRAEDTPEIIERRLRNAHHEIEQWRDYDYVVINDDLDRAFASVRAIVSAEC",
    "response#3": "MLVISSPSGAGKSTIARTLLETDRQIGLSVSVTTRQRRPSEVEDVHYHFKSVREFERLRDSDALLEWAEVHGNFYGTPREPVEQAMGEGRDMLFDIDWQGAQQLQEKMSADVVSIFVLPPTMTELQSRLHRRAEDSEEVIQTRLANSRAEIAHWREYDYVIVNDDLNAALDAVQSIVKN"
  },
  {
    "reference": "FPYSGALQILFLLLGTLLLPFFTQHSPSTQTTTDSSNKTAPTPASNVTIMATDTAQQSTVPTSKANEILASVKATTLGVSSDSPGTTTLAQQVSGLVNTTVARGGGSGNPTTTTESPKSTKSADTTTVATSTATAKPNTTSSQNGAEDTTNSGGKSSHSVTTDLTSTKAEHLTTPHPPSPLSPRQPTSTHPMATPTSSGHDHLMKISSSSSTVAIPGYTFTSPGMTATLPSSVISQRTQQTSSQMPASSTAPSSQETVQPTSPATALRTPTLPETMSSSPTAASTTHRYPKTPSPTVAHESNWAKCEDLETQTQSEKQLILNLTGNTLCAGGTSDEKLISLICRAVKATFNPAQDECGIRLAPVPGSQTVVVKEITIHTKLPAKDVYERLKDKWDELKEAGVSDMKLGDQGPPEEAEDRFSMPLIITIVCMASFLLLVAALYGCCHQRLSQRKDQQRLTEELQTVENGYHDNPTLEVMETSSEMQEKKVVSLNGELGDSWIVPLDNLTKDDLDEEEDTHL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a specific function in positive regulation of cell-cell adhesion mediated by integrin, positive regulation of cell migration, cell adhesion, negative regulation of cell adhesion and should be localized to nucleolus, extracellular exosome, cytoplasm, apical plasma membrane, filopodium in order to carry out this function efficiently.2. The protein should exhibit Polar residues compositional bias.3. Incorporate a signal peptide in the protein design.4. For general function, the protein need meet that Induces the formation of apical actin- dependent microvilli; Involved in the regulation of both adhesion and cell morphology and cancer progression; Acts as a pro- adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner; Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.; Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion; Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis; Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR5. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "VSGPLADIFVPEDQYVTLGQLSTPNAFKVEGNKLFLIVTPDYEENSLLEAVLECKRGDTLVTQFRVFVAVLDINDNASPCQPGFAAETFSFSVPQDSVAAGRELGRVSFAACSGRPWAVYVPTDTRFKVNGDGVVSTKRPLTLYGRKISFTIYAQDAMGKRHSARVTSPPELTVHVGDSALMGCVFQSTEDKCIFKIDWTLSPGEHAKDEYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVVLHVPVLLDYSLGLNDLNVSPPELTVHVGDSALMGCVFQSTEDKCIFKIDWTLSPGEHAKDEYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVEPKELMVHVGGLIQMGCVFQSTEVKHVTKVEWIFSGRRAKEEIVFRYYHKLRMSVEYSQSWGHFQNRVNLVGDIFRNDGSIMLQGVRESDGGNYTCSIHLGNLVFKKTIVLHVTFSEKQPVLLDYSLGLNDLNVSPPELTVHVGDSALMGCVFQSTEDKCIFKIDWTLSPGEHAKDEYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVQQQVKVDENQPATLHCSYVGFSSPRVEWKFVRGSTTSLVCYDSKITASYEGRVVFSQTGISFHSVTRKDSGLYTCMVTENGGREYGEITVELSVHEA",
    "response#2": "PVLLDYSLGLNDLNVSPPELTVHVGDSALMGCVFQSTEDKCIFKIDWTLSPGEHAKDEYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVPTVSIPSSATIGNRVVLTCSEEDGSPPPQYQWFKDGILMPLDPKKTRAFINSSYTIDSKTGELVFEPLSAFDTGEYSCQAYNGVGNAKTSEIVATYTLQVLSKEPVLLDYSLGLNDLNVSPPELTVHVGDSALMGCVFQSTEDKCIFKIDWTLSPGEHAKDEYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVPVLLDYSLGLNDLNVSPPELTVHVGDSALMGCVFQSTEDKCIFKIDWTLSPGEHAKDEYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVTVNVHAPPVLLDYSLGLNDLNVSPPELTVHVGDSALMGCVFQSTEDKCIFKIDWTLSPGEHAKDEYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVTVKVHAPTNLKGYIEENAKPGTSVMTVNATDADDAVNTDNGIVSYSIVSQQPPRPHPQMFTIDPAKGIISVLGTGLDRETTPNYTLIVQATDQEGKGLSNTATAIIEVHAXIXAPANLK",
    "response#3": "MKKGSSGYGFNLHSDKSKPGQYIRAVDPNSPAEASGLQAQDRIVEVNGVCMEGKQHGDVVAAIKAGGEETKLLVPVLLDYSLGLNDLNVSPPELTVHVGDSALMGCVFQSTEDKCIFKIDWTLSPGEHAKDEYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVGGVTVYTSQQQVKVDENQPATLHCSYVGFSSPRVEWKFVRGSTTSLVCYDSKITASYEGRVVFSQTGISFHSVTRKDSGLYTCMVTENGGREYGEITVTWKN"
  },
  {
    "reference": "MSHVLVNVAWPYANGPRHIGHVAGFGVPSDVYARYERMKGNDVLMVSGTDEHGTPILVEAEKEGLTAQELANRYNRVIAKDLCDLGLSYDLFTRTTTGNHEHVVQEMFKQCLENGYIYKGSQQVAISPSTGRTLPDRYIEGECPICHAEGARGDQCDACGNELDPDELINPVSKINGETPRFEQTEHYFLDLPALAEANKAWLETRKGWRTNVINFSLGLFKEVKPRAITRDIDWGIPVPVKGWIDNPNKKLYVWFDAVIGYLSASIEWARRQGDPEKWREWWNDPKCPAYYFMGKDNITFHSQIWPSEMLAYNGKGSKGGETGPMGPLNLPEQVVASEFMTMEGKKFSSSRGIVIYVKDILARYPVDAVRYYISVAGPESSDSDFTWAEFVRHNNEELASSWGNLVNRVANLINKNFGEIPPLDEDSMTNEDRGLLEEASAAFDVVGSSIETHHQKHALSEAMRVVGDINKYISATEPWKIKDDQARLGTVLHVAAQAVSDANHLLAPFLPHSAQKVWEALGGTGTFSPLPELKEVEDLDKPGFTYPIITGDYELGVNVHPWKSEAIEVGAVVPKPAPIFAKIPTEAVEEELARFDEALAARRAAEAERLAAEKAKLAAE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein with methionine-tRNA ligase activity functions is required to enable methionyl-tRNA aminoacylation.2. The protein design should include a 'KMSKS' region motif to ensure proper function.3. The designed protein should have a high specificity for ATP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "NSELVAGWGNLVNRTASLIAKNVGEIPAAGELTDADRDLLATTSGGFSTVGGFIEAHRQRAGIGEAMRVVGEVNKYLTEQEPWKIKNSDPERMKSVLHVTAQAVSDCRTLLSPFLPHSAQKVHQALN",
    "response#2": "NSELVAGWGNLVNRTASLIAKNVGEIPAAGELTDADRDLLATTSGGFSTVGGFIEAHRQRAGIGEAMRVVGEVNKYLTEQEPWKIKNSDPERMKSVLHVTAQAVSDCRTLLSPFLPHSAQKVHQAL",
    "response#3": "VNSDLVGKVVNLASRVGKFASRTGLAPSYPEDGGLFQAAAAKGDEIASAYEDGEFSKAMRLIMELADAANPFVEHAKPWEMNKAPERQDELRDVCTVALNLFRQLAVYLAPVLPELAKKCGDLV"
  },
  {
    "reference": "MGGKWSKSKGWSAVRERMKRTQPEPAATGVGAASRDLEKYGALTSNNTAANNANCAWLEAQEEEEVGFPVKPQVPLRPMTYKAAFDLSHFLREKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGVRFPLTFGWCFKLVPVDPDKVEEATEGENNHLLHPMNQHGIEDPEREVLQWKFDSRLAFHHMARELHPEYYKDC",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design must feature the PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Diacidic; necessary for CD4 internalization motif.2. The designed protein should be involved in suppression by virus of host autophagy, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I by localizing to virion component, extracellular region, membrane, host cell Golgi membrane and exhibiting SH3 domain binding, GTP binding.3. The protein must exhibit the following characteristics: Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1. The designed protein sequence is ",
    "response#1": "MGGKWSKSSLVGWPAIRERIRRTEPAAEGVGAASRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLVYSQKRQEILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPDKVEEATEGENNSLLHPMSQHGMDDPEKEVLVWKFDSRLAFHHMAREMHPEYYKNC",
    "response#2": "MGGKWSKSSLVGWPAIRERMRRAEPAAEGVGAASRDLEKHGALTSSNTAANNADCAWLEAQAQGEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYNTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEQEQVEKANEGENNSLLHPMSQHGMDDPEKEVLMWKFDSRLAFHHMARELHPEYYKNC",
    "response#3": "MGGKWSKSSLVGWPAVRERIRRTEPAADGVGAVSRDLERHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTFKAAFDLSFFLKEKGGLDGLIYSKRRQEILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVDPEEVEEANKGENNCLLHPICQHGMEDEEREVLKWKFDSHLAHTHKARELHPEFYKDC"
  },
  {
    "reference": "MRHTLSELGLTGPSDIRVGGLAVDNRKVVPGDLFAALPGTETHGARFGADAVERGAVAVLTDPAGAAMLPDGIPSIVVEDPRAALAFAAALMAGAQPGVMVAVTGTNGKTSVASFTRQLWEAMDIAGANIGTTGVEGAFHAPSAHTTPEPITLHRLLADMAAAGVTHAAMEASSHGLDQRRLEAVRLAAAGFTNLTQDHLDYHGTMEAYLAAKAGLFDRLLPGDGVAVINLDDPYGPDLAANCEARAQEVIGVGRNEAARIRLTGQRFDATGQEVLFRWQDRGHSVRLNLIGGFQAANALLAAGLVIGAGEDAAATFAALPKLAGVRGRMELAATKADGAAVFVDYAHTPDAVATALTALRPHVMGRIVAIVGAGGDRDTTKRPLMGTAAADHADVVIVTDDNPRSEDPATIRAAVMEGAPDAIEVGDRARAILEGAAMLEPGDALLIMGKGHESGQTVGDMVLPFDDVEQASISVAALEGRDA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should contain at least one Meso-diaminopimelate recognition motif motif to achieve its intended function.2. A protein that can modulate the overall flux of metabolites through the peptidoglycan biosynthesis, Cell wall biogenesis pathway, enhancing or suppressing its activity as needed.3. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..4. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding.5. A protein that can perform ATP binding, magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity in cytoplasm to support cell division, regulation of cell shape, peptidoglycan biosynthetic process, cell cycle. The designed protein sequence is ",
    "response#1": "MKMKDLLYKIPIISIIGDTNIDISGISADSRKVTPGMLFVAVAGTKADGAAYAADAVARGAVAVVAGHAVEANVPVFTVSNPRRFLALASARFYGKPSDKLQVVGVTGTTGKTTTTYLIKSILEKYGRKAGLIGTIVYQIGTNLISSQRTTPESLDLEIMFRDLLKTGAHYAVMEVSSHSLMLHRVDCIEFDQAIFTNLGLDHLDFHNTMEEYLNAKVKLFRHLSTFKEKENKFAIVNLDDPHSREVLEAASAKKITYGFSEKAQVYAKPLSMDIGGITFELFTPEGTRQINMKLTGKFNIYNALAATAYGTAMLLGMEQEELLELSAELKAVEGRMELAARLPNGAAAYVDYAHTPDALERLLTALRPHTTGRLHVVFGAGGDRDGGKRPLMGEVCARLADHAILTDDNPRSEDPAAIRAAVRAGMAGAAEVTEIGDREAAIAAGLAALGPGDVLAVAGKGHETYQEIKGVKHHFDDKEVIKEIFS",
    "response#2": "MKLSEILPFIDFQIIQGSTDILVTSITSDSRLVGEGSLFLGLPGERFDGGRFWRQALEAGAAAAVIGPSAAQELPPAADQPVLVLREPVARSIGELAARFYGYPAKQLKVIGVTGTTGKTTTTYLIKSILEKYGRKAGLIGTIVYQIGTNLISSQRTTPESLDLEIMFRDLLKTGAHYAVMEVSSHSLMLHRVDCIEFDQAIFTNLGLDHLDFHNTMEEYLNAKVKLFRHLSTFKEKENKFAIVNLDDPHSREVLEAASAKKITYGFSEKAQVYAKPLSMDIGGITFELFTPEGTRQINMKLTGKFNIYNALAATAYGTAVLLGLPLEATMQALSHLKAVPGRMELAARLPNGAAAYVDYAHTPDALERLLTALRPHTTGRLHVVFGAGGDRDGGKRPLMGEVCARLADHAILTDDNPRSEDPAAIRAAVRAGMAGAAEVTEIGDREAAIAAGLAALGPGDVLAVAGKGHETYQIIGQEVLPFDDVAQARQAIQKRFGE",
    "response#3": "MRLIELVQDLPYKCIEGNVDREISGISADSRKVTPGMLFVAVAGTKADGAAYAADAVARGAVAVVAGHAVEANVPVFTVSNPRRFLALASARFYGNPSESIKLIGVTGTNGKTTTATLIYQLLNNLGKKTGMIGTIKNRIGDKILPTQNTTPESFDIQRYFAEMAEEGCEYCVMEVSSHALELKRVEGCLFDAGVFTNLTQDHLDFHGTMENYKNAKLKLFSLLENKSKVAVINIDDEYGKEIIGNTEAEIIRFGFSENSDIRGENLIFSPDGIKFDIITNSGEINLNVGLIGKFNVYNALAAITAALIEGISLETIKKGLENVKGVPGRFETVDEGQDFGVIVDYAHTPDALERLLKTVREIGVKRIITVFGCGGDRDRGKRPQMGQIAANLSDWAIVTSDNPRTEDPNQIIADILEGISTSNIDVEIDRRKAIYRAVEIAQTGDAVVIAGKGHETYQIFKDKTIHFDDREVARNAIRERKN"
  },
  {
    "reference": "MRLSQLLERLEYEVVQGTEDIAITTLINDSRKVEPGSVFVCISGAVSDGHRFATEVADKGAAALIVEKEVEVPGDVTVIRVEDTRYALALMSAAYFGYPANKLKIIGITGTKGKTTTTYMIRSILEEVGRKVGLIGTIEAIIGEKHIPAANTTPESYTIQKYFAEMVEAGCDTVVMEVSSQGLMLNRTAGIPFEIGIFTNLGKDHIGPNEHADFEDYKRCKGLLFKQCELGIANGDDPWFEDVFKEATCKVETFGFEEKNDLRAVDTRLVSRPGYLGVAYHVAGLMDFDVEIDVPGTFSVYNSLTAIAVCRHFGVPEEVIKSALKVAKVKGRIEMVKVSDEFTLMIDYAHNAMSLESLLTTLKEYHPSRLVCLFGCGGNRSKDRRYEMGEVSGRLADLTIITSDNPRFEDPQAIIDDIKQGIGKTDGKYVEICDRKEAIRYAIEHGQPGDVIVLAGKGHEDYQEIEGVKHPMDERVLIQEVLEELGK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.2. We require a protein that can specifically bind to the metabolites produced in the peptidoglycan biosynthesis pathway.3. The ideal protein would be one that plays a critical role in cell division, peptidoglycan biosynthetic process through ATP binding, tetrahydrofolylpolyglutamate synthase activity, magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity within cytoplasm.4. The protein should have a high affinity for ATP, meso-2,6-diaminoheptanedioate, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate.5. The protein contains novel Mur ligase C-terminal domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MKLKELLKNISITKIQGNVDVNKIEYDSRKVEKGDLFICIEGYSTDGHKYAKSAYEKGANIIVCQKDLDNLSYYKDCTIIKVDDTRKALAILASNYYDNPSSKLQLVGVTGTNGKTTCCFLITQTLQKLNCKSALMGTLGYGMADKVLQTQNLTTPDAVTLHQNYAALLEEGAKVIAMEVSSHGLDQNRLNGLKIDSGIFTNLTQDHLDYHKDMNTYLKAKQKLFERPELKRAIINLDSPYFDKVLAKINPKLPIFIYSLNQPKLLRRSYPVSTSFIYAKHYSFHNNGIVAEVVTPWGEGELRSPLMGDFNLSNLLAVYGTAIQLGLEELEILQLVSNLTSVSGRIEMVPVSDQFTLLIDYAHNAMSLKSLLTTLREYQPKRLVCVFGCGGNRSRIRRFEMGEVSGNYADFTIITSDNPRFEKPQDIIEDIKTGINKTSGRYMEICDRKEAIAYAIGHGQPGDIIVLAGKGHETYQEIKGVRHHFDDKEELNQAFENTK",
    "response#2": "MARRPPVSPGSAVEVLLGQLVPEARVRSVTNDSREVEEGALFVAVRGFADDGRRFIAQAVERGAVAVLSEVTGTSGKTTTVFLLDAGLRAAGIETGLVGGVEIRVGGDRVPSALTTPESTDLHALMAMMRERGVGAAAMEVSSHALKQGRVGGMTYEVAVFTNLSQDHLDYHPSMQDYFFTKARLFTPEYSRVGVVNLDDHHGRELLEIATVPVTTFSASGDPAADWRAENVRVGADGSVFRVVGPGGIEADGEVRLAGPFNVANALAAVVLGATCGVDQEALEAALTSFAGLPGRLELVGQRSGAPIFVDYAHKPDALAKALQALRPYAKGRLVVVIGAGGDRDAGKRPLMGAIAAEHADRVIVTDDNPRSEDPAAIRAAILAAAPGAAEIGDREKAIRAAIAALQPGDALLIAGKGHEDYQILGTEKIHFSDRETARDALARRRGDR",
    "response#3": "MKISGLSSDSRKTKPGDLFAALTGSQSDGRAYVDQAVAAGAVAVLAEPGLPPCGVPVIEVPHARAALSRAASRFYGEPSRALTLVGITGTNGKTTTSYLIESIMAAAGIATAVLGTVSYRLGATVLPASHTTPESTELQEVIRQLVDRGAAGVVMEVSSHALEQRRVDGCQFDVGVFTNLTRDHLDYHGSMEQYLEAKLRLFREFLQPSPNKPNRRAVVNLDDEYGEVVARESVCPVITYGIAKGDVRAVDVHSSISGITGKVVTPSGTIEFSSGLVGRFNLYNILAALATGLSLGISTETLCSVLPKLVGAPGRLELVGQRSGAPIFVDYAHKPDALAKALQALRPYAKGRLVVVIGAGGDRDAGKRPLMGAIAAEHADRVIVTDDNPRSEDPAAIRAAILAAAPGAAEIGDREKAIRAAIAALQPGDALLIAGKGHEDYQIIGTTKHHFDDREEARAALLEAGL"
  },
  {
    "reference": "MSLSIVILAAGKGTRMKSTTTKVMHALAGKPLVEHVIDTANKLKPTQLTVVTGNGAEQVQTLLDHRNITSALQAEQKGTGHAVMQAQDIIANSDQVLVLYGDVPLTQQATLASLIESGNQNSLRILTTKLDNPTGYGRIVRNDAGQVICITEQKDADEQTLLINEVNSGIMVLPSQWLIESLPKLENNNAQGEYYLTDMVALAVADGIAINTVCCEDNAEVAGVNTRLQLAELERHYQRSQAELLMTNGVTLADPARFDVRGDITVGQDVTIDINVILQGKVTIADNVTIGANCIIKDSTIAAGTEILANSMLDNAVVGEHVSIGPFARLRPGTVLANKAKIGNFVETKNSNIGLGSKVNHLSYIGDTDMGKNVNIGAGTITCNYDGANKHRTVIGDNAFIGSDSQLVAPVTVAEGATIGAGTTLRKDAEAEALTLTKSVQRTVSGWKRPVKKK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein localizes to the cytoplasm. The designed protein sequence is ",
    "response#1": "CCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGK",
    "response#2": "CCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGK",
    "response#3": "CCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGK"
  },
  {
    "reference": "MVCIHTENQNQGDFYPFVLLEISVLHESPLGHLRYRLTDVPPQPNSPGEIYCFYINCIMNRR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Involved in modulation of rDNA transcription. The designed protein sequence is ",
    "response#1": "GRQSLGFSVFNNKNGDLLRSKKNLASGTSVIEVNPGNCNEFLICFINLVYDGSWSSIDTEKSVTIKMTYNDKLDPDMLLHLVNQMTPQVVKALNTVSDGLFQIVSDTTLLQMESDRRDINEATYSYLIVGFVSLMVAQLISNIIVTTV",
    "response#2": "GRQSLGFSVFNNKNGDLLRSKKNLASGTSVIEVNPGNCNEFLICFINLVYDGSWSSIDTEKSVTIKMTYNDKLDPDMLLHLVNQMTPQVVKALNTVSDGLFQIVSDTTLLQMESDRRDINEATYSYLIVGFVSLMVAQLISNIIVTTQ",
    "response#3": "GRQSLGFSVFNNKNGDLLRSKKNLASGTSVIEVNPGNCNEFLICFINLVYDGSWSSIDTEKSVTIKMTYNDKLDPDMLLHLVNQMTPQVVKALNTVSDGLFQIVSDTTLLQMESDRRDINEATYSYLIVGFVSLMVAQLISNIIVTTC"
  },
  {
    "reference": "MLLLISPINHEEALESIKGGADIVDVKNPKEGSLGANFPWVIRDIREITPEDKLVSATLGDVPYKPGTVSLAAMGAHVSGADYIKVGLYGTKDYDEAVEVMENVAKTIKDVDNDTIVVASGYADAHRVGAVDPMEIPKVAKDAGCDLAMLDTAVKDGHTLFDYLSIEDLEKFVNEAHSYGLKTALAGSVKKEQLKPLNDIGCDVVGIRGAACVGGDRNTGKIHHTAVAELKELCDSF",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Catalyzes the formation of 4-(hydroxymethyl)-2- furancarboxaldehyde phosphate (4-HFC-P) from two molecules of glyceraldehyde-3-P (GA-3-P)..2. The protein design should take into account the physiological conditions of the Cofactor biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MTSTG",
    "response#2": "GVM",
    "response#3": "LVLLPG"
  },
  {
    "reference": "MLKIGSHVSMSGKKMLLAASEEAVSYGATTFMIYTGAPQNTRRKPIEELNIEAGRKHMELNGIEEIIVHAPYIINVGNTTKPETFQLGVDFLRMEIERTSALGVAKQIVLHPGAHVGAGADAGIQQIIKGLNEVLTPEQTVNIALETMAGKGTECGRSFEEIAKIIDGVKYNEKLSVCFDTCHTHDAGYDIVNDFDGVLNEFDKIVGIDRLQVLHINDSKNVRGAGKDRHENIGFGHIGYKALHHIVHHPQLMHVPKILETPYVGEDKKDKKPPYKLEIEMLKNGTFDEGLLEKIKAQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must be able to DNA binding, DNA-(apurinic or apyrimidinic site) endonuclease activity.2. The protein must exhibit the following characteristics: It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate..3. The protein should have a high affinity for Zn(2+). The designed protein sequence is ",
    "response#1": "PELPEVETVRRGLEQKLKNFIIKRVEICRESTVAYPIDKIDFIEGLQNSLLYKWNRRGKYLIAELKKTVSNNNDANEISFVENGVLVVHLRMTGYFTFNNTLTNPCKHTRIRLFDNNNNELRYIDVRSFGQML",
    "response#2": "PELPEVETIRRSLSQHILERRIEEILIRWPGAVEGYEEKTFADAVRGLKFQSIERRGKYLLFTLEEGWSFIAHMRMTGRMVYHAQSQEPEKHTHVVLKLSSGEIHFTDTRKFGRLV",
    "response#3": "ISFNINGLRARPHQLEAIIEKYQPDVIGLQEIKVADEAFPYEITENLGYHVFHHGQKGHYGVALLTKQEPKVIRRGFPTDNEDAQKRIIMADLETEFGLLTVINGYFPQGESRAHETKFPAKEKFYADLQQYLEKEHDKSNPILIMGDMNISPSDLDIGIGDENRKRWLRTGKCSFLPEERAWYQRLYDYGLEDSFRKLNPTANDKFSWFDYRSKGFDDNRGLRIDHILVSQKLAERCVDVGIALDIRAMEKPSDPELPEVETVRRGLEQKLKNFIIKRVEICRESTVAYPIDKIDFIEGLQNSLLYKWNRRGKYLIAELKKTVSNNNDANEISFVENGVLVVHLRMTGYFTFNNTLTNPCKHTRIRLFDNNNNELRYIDVRSFGQMV"
  },
  {
    "reference": "MTDTAKQCPFTGANTVKGTSVTEQWWPEQLNLKILHQHSEQVNPMGESFDYAVEFESLDLAAVKQDIAHALVDSKDWWPADYGHYGPLMIRLTWHAAGTYRIGDGRGGGGTGAQRFAPLNSWPDNVSLDKARRVLWPIKQKYGCKLSWADLLILAGNVALESMGFKTFGFGGGRPDIWGTEEDIYWGSENEWLGDERYSGERSLETPLGAVQMGLIYVNPEGPNANPDPIAAGIDIRETFARMAMNDEETVALIAGGHTFGKAHGAAPDTYVGDDPEAAAIEDQGFGWRNAFGTGVGGDAITSGLEGAWTSNPIVWDNGYFDNLFGYEWELTKSPAGAQQWRPTDRAADDSVPDAHDPNKRHAPIMLTTDLALRFDPIYEPISRRFHEHPEELADAFARAWFKLTHRDMGPVSRYLGPEVPAEQLIWQDPVPVTQGRPLGDGDIVSLKQRVLNSGLSVSQLVATAWASASTFRGTDKRGGANGARIRLAPQNGWDVNIAAGVSEVIAVLEQVQADFNSQGGAQVSLADLIVLGGCAAIEKAANDAGHSVEVPFSSGRTDATPDQTDVESFAVLEPTADGFRNYQADFHKRPAEELLVDKAFLLNLTAPEMTVLVGGMRALDANVGQSPHGVLTNRPGALSNDFFTNLLSMDTEWKVSADRVGIYEGRDRASGELRYTATAADLVFGSNSQLRAIAEVYGCDDGAARFVNDFVAAWHKVMNLDRFDLP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must have high specificity towards heme binding, catalase activity. The designed protein sequence is ",
    "response#1": "AFARAWFKLTHRDMGPRSRYIGPEVPGEELIWQDPVPAVDHKLIDGQDAAALKKSILASGLSVAQLVSTAWASATTYRNSDMRGGTNGARIRLAPQKDWEVNQPDQLSSILQTLETIQQDFNAVAQDGKRVSVADVIVLAGCAGVEQAARNAGREVSVPFEPGRTDASLDQTDVESFAVLEPTADGFRNYLKAKHTVSPEELLIDKAQLMTLTAPEMTVLVGGMRVLNANASQVQDGVFTDRPESLSNDFFVNLLDMGIKWVPTSKECYAFEGRDRATGGLKWSATRVDLIFGSNSQLRAIAEHYAR",
    "response#2": "TFARAWFKLTHRDMGPKARYIGPDVPQEDLIWQDPVPNGNANYDIDAVKAKIAASGLSVSDMVTTAWDSARTFRQSDKRGGANGARVRLAPQKDWQGNEPERLARVLPVLENIAKDTGASVADVVVLAGNVGIEQAASAAGVNVTVPFLPGRGDATQEMTDVESFEVLEPLHDGYRNWLKQNYVVTPEEMLLDRTQLMGLTAAEMTVLVGGMRVLGTNHGGSKHGVFTDRVGQLTNDFFINLTDMKYTWEPVGENLYEIRSRRSKDVKWTATRVDLVFGSNSILRAYAELYAQY",
    "response#3": "AFARAWFKLTHRDMGPRSRYIGPEVPGEELIWQDPVPAVDHKLIDGQDAAALKKSILASGLSVAQLVSTAWASATTYRNSDMRGGTNGARIRLAPQKDWEVNQPDQLSSILQTLETIQQDFNAVAQDGKRVSVADVIVLAGCAGVEQAARNAGREVSVPFEPGRTDASLDQTDVESFAVLEPTADGFRNYLKAKHTVSPEELLIDKAQLMTLTAPEMTVLVGGMRVLNANASQVQDGVFTDRPESLSNDFFVNLLDMGIKWVPTSKECYAFEGRDRATGGLKWSATRVDLIFGSNSQLRAIAEHYAR"
  },
  {
    "reference": "MDRRTVLKSTHIQQKIAEKKAELENLNEIKKFTSILTNQLDELENKLDYMAEGTESVALVLSNWQNVVKSVSLASLGLLKYSEKDYETGAPLPECLVRIKLDKDDNVGEEIQEKEEMETDDYISDQQAEESENINEEEDRQEVQTSYTD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be optimized for stability and expression in cytoplasm, DASH complex, mitotic spindle where it will participate in cell division.2. Incorporate a signal peptide in the protein design.3. The protein must exhibit the following characteristics: The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore .. The designed protein sequence is ",
    "response#1": "ETVDKLEAMFQKAEADIEYVEKRLKFDFMASAREAGTFEGNPVELLEDLSAIKARHAALCAQVEEIAAEQKRSMDSIRAHLDTTVQLVQQLQNTADVQVPPLTEEEQEARDALD",
    "response#2": "ETVDKLEAMFQKAEADIEYVEKRLKFDFMASAREAGTFEGNPVELLEDLSAIKARHAALCAQVEEIAAEQKRSMDSIRAHLDTTVQLVQQLQNTADVQVPPLTEEEQEARDAL",
    "response#3": "ETVDKLEAMFQKAEADIEYVEKRLKFDFMASAREAGTFEGNPVELLEDLSAIKARHAALCAQVEEIAAEQKRSMDSIRAHLDTTVQLVQQLQNTADVQVPPLTEEEQEARDAR"
  },
  {
    "reference": "MALENTENNTGCEIIPSRTTLKNFTKDYATYLDELDLLPHSKNLFRIPTKKHLKSNTTIGPLLVSTDEQEDIRDSSSPDSIYLCGNSLGLQPRRTASLILHYLTTWATQGVHGHFKPLEDSPLPEWLDVDIRASNSMAPILGALPSEVVVMQTLTANLHLLMSAFYRPDFHGRHKIIIESQAFPSDHFVAESQIKLHGLDPKDSLIIITPQAPSETLTTDQIISTIEEHGPTTSVILFPGIQYYTGQLFDIQAINAAAQKAGIFAIWDLAHAAGNVDLRLHDWNIDAAVWCNYKYLNSGPGVSGGLFIHENHSNVSEDHGETKYINRLSGWWGSEKKSRFAMDNRFKPIPGAAGFQLSNPSVLDLTSVIGSLEVFHEACRWQSTDHARQEGITPLRKKSIRLTGYLEAGLHNMEMFKKGMFHIITPSDPNQRGAQLSLKLKPDLLNIVMKELEERGIVVDERKPDVIRVAPNPLYNTFEDCWNFIDAFQEALIIAVKAKMENLLQKKNQES",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must be engineered to have a high affinity for pyridoxal 5'-phosphate in order to increase its catalytic activity.2. The designed protein should have a high specificity for pyridoxal 5'-phosphate binding, with minimal off-target effects.3. For general function, the protein need meet that Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively.4. The protein design must be able to perform 3-hydroxykynureninase activity, kynureninase activity in order to contribute to L-kynurenine catabolic process, 'de novo' NAD biosynthetic process from tryptophan, tryptophan catabolic process.5. The protein design should take into account the physiological conditions of the quinolinate from L-kynurenine: step 2/3, Cofactor biosynthesis pathway. The designed protein sequence is ",
    "response#1": "SPDSDHTISTEKILATIDAHAHEIALVLLPGVQYYSGQFLDIGRITEHAHSKGLTIGWDLAHAAGNVPLKLHDWNVDFAAWCTYKYMNAGPGAIAGLA",
    "response#2": "MEPRPGEHTLREEDILKVIAEQGSTIALVIFSGVQYYTGQWFPMERITKAAKAQGCICGWDLAHAVGNVPLSLHDWGVDWAVWCSYKYLNSGPGGIAGLFVHESWDDRHEVFLRRSCMEFAAYCRHPRPGEHTLREEDILKVIAEQGSTIALVIFSGVQYYTGQWFPMERITKAAKAQGCICGWDLAHAVGNVPLSLHDWGVDWAVWCSYKYLNSGPGGIAGLFVHESWDDRHEVFLRRSCMEFAAYCRHDVDLLDVLYEDLRYFFAHMDYALNYTRPPEPEGPVDWERAEEYRRRLEEGVRLRKRCPRDWTACERDARECVRTAMSTGFLGYFLCRDAPRHPLSLLAFWRALEVDLGTRFGGPRPGEHTLREEDILKVIAEQGSTIALVIFSGVQYYTGQWFPMERITKAAKAQGCICGWDLAHAVGNVPLSLHDWGVDWAVWCSYKYLNSGPGGIAGLFVHESWDDRHEVFLRRSCMEFAAYCRHDVDLLDVLYEDLRYFFAHMDYALNYTRPPEPEGPVDWERAEEYRRRLEEGVRLRKRCPRDWTACERDARECVRSAMSTGFLGYFLCRDAPRHPLSLLAFWRALEVDLGTRFGGYEAPPPEVLYLLRHKE",
    "response#3": "MKKSPDSDHTISTEKILATIDAHAHEIALVLLPGVQYYSGQFLDIGRITEHAHSKGLTIGWDLAHAAGNVPLKLHDWNVDFAAWCTYKYMNAGPGAIAGLAAGAGGMSVATGSPDSDHTISTEKILATIDAHAHEIALVLLPGVQYYSGQFLDIGRITEHAHSKGLTIGWDLAHAAGNVPLKLHDWNVDFAAWCTYKYMNAGPGAIAGLGERGVFEPLTLKRAGGVVGPQPDVYATLERAVGQGGVYFAEALWGRTASLPQFEVQAFSSGVDARLKAAQIAGIEAALKG"
  },
  {
    "reference": "MSSFGKVAVLFGGTSAEREVSLNSGSRVLAALQGQGIDAHAFDPAEQPLDALKGYDRAFIALHGRHGEDGTIQGALEVMHIPYTGSGVLASALAMDKFRTKLMWRAAGLAIPEYALLTADSDFADIEEELGLPLFVKPAREGSSIGVTKVKERGALKAAYEEAARHDPLVIAEKGVMGGEYTVGIIGDEAMPIIKIEPATEWYDYEAKYNRDDTRYLCPCGLPEAKEMEIRKGALEAFRILGGRGWGRVDFLMDEDGKYYFLEVNTAPGMTDHSLVPMAARVNGMDYPTLVRRVLELAAND",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must have at least one ATP-grasp domain.2. For general function, the protein need meet that Cell wall formation.3. The Mg(2+), ATP binding site should be stable and able to withstand changes in environmental conditions.4. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+), Mn(2+) to enhance enzymatic efficiency.5. We need a protein that can perform ATP + 2 D-alanine = ADP + D-alanyl-D-alanine + H(+) +   phosphate with high efficiency.6. The protein design should consider the endogenous proteins and enzymes present in the peptidoglycan biosynthesis, Cell wall biogenesis pathway to avoid interference with native metabolic processes.7. The designed protein should be involved in regulation of cell shape, peptidoglycan biosynthetic process by localizing to cytoplasm and exhibiting D-alanine-D-alanine ligase activity, ATP binding, metal ion binding. The designed protein sequence is ",
    "response#1": "MATAGVPVAEGLTLTRAEIAKAHVMAPPYVIKPVADGSSVGVFIVTEDQAHPPQELFRDDWPHGEELLVEKYIAGRELTCAVIKGEASDVIEIVPLLKFYDYEAKYSKGASKHVLPAPVLPFVYQEVRRLTLAAHGALGCRGVSRADFRYDDRIEGTGGLVCLEVNTQPGMTETSLVPELAGYAGVAFDELVQWMVKDNTKIYSENSKHLFHSEGLPTPAWIELAGDHEEADELVDHFLGDFHGAAFVKPLDSGSSVGISRAVGKDELIRGVAKALSVSHRCMVERAIEGRELTLSILDGEAFPLIEIVPIDGFYDYDHKYTAGRTNYLVPAPNLDDKSLEAVVKIGLAAYHITGCRGLVRADFILDGEGTPWLLELNTIPGMTELSLAPKAAHAVGIEMPQLAERILEDMEKVDATLFHSEGLPTPAWIELAGDHEEADELVDHFLGDFHGAAFVKPLDSGSSVGISRAVGKDELIRGVAKALSVSHRCMVERAIEGRELTLSILDGEAFPLIEIVPIDGFYDYDHKYTAGRTNYLVPAPNLDDKSLEAVVKIGLAAYHITGCRGLVRADFILDGEGTPWLLELNTIPGMTELSLAPKAAHAVGIEMPQLAERILEDMEKVEATLFHSEGLPTPAWIELAGDHEEADELVDHFLGDFHGAAFVKPLDSGSSVGISRAVGKDELIRGVAKALSVSHRCMVERAIEGRELTLSILDGEAFPLIEIVPIDGFYDYDHKYTAGRTNYLVPAPNLDDKSLEAVVKIGLAAYHITGCRGLVRADFILDGEGTPWLLELNTIPGMTELSLAPKAAHAVGIEMPQLAERIL",
    "response#2": "MATAGVPVAEGLTLTRAEIAKAHVMAPPYVIKPVADGSSVGVFIVTEDQAHPPQELFRDDWPHGEELLVEKYIAGRELTCAVIKGEASDVIEIVPLLKFYDYEAKYSKGASKHVLPAPVLPFVYQEVRRLTLAAHGALGCRGVSRADFRYDDRIEGTGGLVCLEVNTQPGMTETSLVPELAGYAGVAFDELVQWMVRDYYQAPERPWEVLFHSEGLPTPAWIELAGDHEEADELVDHFLGDFHGAAFVKPLDSGSSVGISRAVGKDELIRGVAKALSVSHRCMVERAIEGRELTLSILDGEAFPLIEIVPIDGFYDYDHKYTAGRTNYLVPAPNLDDKSLEAVVKIGLAAYHITGCRGLVRADFILDGEGTPWLLELNTIPGMTELSLAPKAAHAVGIEMPQLAERILPGGDWGKDFILFHSEGLPTPAWIELAGDHEEADELVDHFLGDFHGAAFVKPLDSGSSVGISRAVGKDELIRGVAKALSVSHRCMVERAIEGRELTLSILDGEAFPLIEIVPIDGFYDYDHKYTAGRTNYLVPAPNLDDKSLEAVVKIGLAAYHITGCRGLVRADFILDGEGTPWLLELNTIPGMTELSLAPKAAHAVGIEMPQLAERILPGGEWGENFIGLIFGGNSNEHEVSISSAKTVFQAFKSEINKERFTIRAFYINKYGDWLDSDSSEKILIDESKNKNTTKKQIFNQEKINFLDGVDFQNVDVWFPLLHGINGEDGSIHGLLRFTKKPLVG",
    "response#3": "MATAGVPVAEGLTLTRAEIAKAHVMAPPYVIKPVADGSSVGVFIVTEDQAHPPQELFRDDWPHGEELLVEKYIAGRELTCAVIKGEASDVIEIVPLLKFYDYEAKYSKGASKHVLPAPVLPFVYQEVRRLTLAAHGALGCRGVSRADFRYDDRIEGTGGLVCLEVNTQPGMTETSLVPELAGYAGVAFDELVQWMVDALGPIDAPRILLRDAGLPIVPFVTMTAASPVSYDDAARAVGSSELFVKPANLGSSVGISKTRDAAEFEAACQLALRFDRKILIERCIAPVREIECAVLEHADGQIKASELGEIVPANSHGFYSYEAKYTDANGAALHVPAQVEPAVAQRIRKMATEVFGALCCESLARVDFFVRGDEIYVNEVNTLPGFTNISMYPKMWEAAGLPQPALMDELLQDNYHPNWGIPQGHFLVIYKDEYLSKKEYFIRRIESEFSYPVFVKPANSGSSVGISKAKDKDELILAIHEAFLYDTKILIEQAINAREIECAVLGNSEILVSALGEIVPSREFYSYEAKYIDGSSELIIPAKLEKQVEDEIKELAVKIYKLFECSGMARIDFFVDKETNKVYFNEVNTIPGFTSISMYPKLMEYSGIPYPQLLDKLISAIGPIQAPRICGIPQGHFLVIYKDEYLSKKEYFIRRIESEFSYPVFVKPANSGSSVGISKAKDKDELILAIHEAFLYDTKILIEQAINAREIECAVLGNSEILVSALGEIVPSREFYSYEAKYIDGSSELIIPAKLEKQVEDEIKELAVKIYKLFECSGMARIDFFVDKETNKVYFNEVNTIPGFTSISMYPKLMEYSGIPYPQLLDKLI"
  },
  {
    "reference": "MSNQPWIVHKFGGTSLANAGRFQRVAEILSARSGGRQAIVVSAVGGVTDRLLKLLDSARHRDEEFAKQLAELRADHEHLADELLGSGAIRDRFQEFFDRVCRDIADVCQATLLIGSDSPGVRAVVSGAGELWSAQLLTGLLQKKGRTDEVRMLDARDVLVIRHSELGPDVIWDRSQAALMSHVPADFNGTLVVTGFIARDSDGVQTTLGRNGSDYSASIFGALLNASQVNIWTDVPGVMSADPRLVAESAVIPELTYDEAMELAYFGAKVLHPQTMAPAVDSGIPIYIRDSSMPETAGTRIGSKALSAATVKGITAIDGMALVNLEGAGMIGVPGTAWRLFGTLRDAGISVVLISQGSSEHSICFAVKAEHAEQVREILTTAFRPELDSGQIHSLDVTPGCSILAVVGDGMAGIPGVAAKVFGSLGRAGINVRAIAQGSSERNISAVIDQADTLRALRAVHAGFYLSPQTLSIGLIGPGNVGTVLLEQIAGQAKRLSSELNLDLRIRGLASSRKMLLDDIAIGLDDWRDRLGASNEALDMDRFVDHIKVDHIPHAVIIDCTASQQVADRYADWLAKGIHVVTPNKKAGSGEFSTYQRLAAAQRTGDSRFLYETTVGAALPIMQTLRDLRETGDQVHRIQGIFSGTLAYLFNVYDGSRPFSELVAEARSHGYTEPDPRDDLSGTDVARKVVILGREMGLDLELDSIRLKGLVPESLVDCSIDEFLTGLREFDSDIASLYKETKEGGSVLRYIGRLESDGRATVGLEQIP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. We require a protein that can specifically bind to the metabolites produced in the Amino-acid biosynthesis, L-threonine from L-aspartate: step 3/5, L-threonine biosynthesis pathway.2. The designed protein should contain one or more ACT domains that are essential for its function.3. We are looking for a protein with broad substrate specificity for L-homoserine + NADP(+) = H(+) + L-aspartate 4-semialdehyde +   NADPH.4. The designed protein should be optimized for ATP binding, NADP binding, aspartate kinase activity, homoserine dehydrogenase activity activity in order to improve phosphorylation, threonine biosynthetic process, lysine biosynthetic process via diaminopimelate. The designed protein sequence is ",
    "response#1": "MKVMKFGGTSLANWPRFLMAADIIADSSATEPVATVLSAPATITNALLEMVDLAVSGKDFQTVIDHIETVFTDLYTQAGESLLSDPQQDLLTQKLEQQMLMWKQKLEGISLLGECPDSVRASVVVSGEKLSAALMEQVMLAKNITASVLDPVALFLAHGQPLESVVDIIVSKPRFGLLPLNDYQVWVMPGFTAGDVHGKVVTLGRNGSDYSAAVLAACLDASSCEIWTDVDGVYNTDPRVVTDAKLLSQLSYQEAMELSYFGAKVLHPKTIAPIAQYHIPCYIKNSFNPKAPGTLVSNDDDKTGLQVKAISNLDNQTMFNVSGPGMKGMVGMASRTLAAISRSGISVSLITQSSSEYSISFCVATSDAHKAKCALEQEFELEIKSELLEPIELREGLAIVSLIGDGMKTHKGVAAKFFSALAKASVNIVAIAQGSSERSISTVIQQSKTKHAIGACHQAFFDVQQYVDVFLVGAGNVGAGLLEQIKQQNEMLKELHISIRVCGIANSRQMLLDSNGIDLANWQSLLAQQGQAYDLAGLLLWGQEQQLLNPVLVDCTSSEQVSDGYLDVMNAGFHVVTPNKKANTRDYDYYQALRQVSLSQRRQFLYETTVGAGLPVIDNLKKLLCAGDKLHKFNGVLSGSLSFIFGMLDEGMSLSQATTIARDKCFTEPDPRDDLSGMDVARKVLILAREVGLQLELSDINIESVLPADFDDSGDVNTFMANLPSLDAQVSQRVANAKAQGKVLRYVGQIDDGKCHVRVVEVDAYDPS",
    "response#2": "MKVMKFGGTSLANWPRFLMAADIIADSSATEPVATVLSAPATITNALLEMVDLAVSGKDFQTVIDHIETVFTDLYTQAGESLLSDPQQDLLTQKLEQQMLMWKQKLEGISLLGECPDSVRASVVVSGEKLSAALMEQVMLAKNITASVLDPVALFLAHGQPLESVVDIIVSKPRFGLLPLNDYQVWVMPGFTAGDVHGKVVTLGRNGSDYSAAVLAACLDASSCEIWTDVDGVYNTDPRVVTDAKLLSQLSYQEAMELSYFGAKVLHPKTIAPIAQYHIPCYIKNSFNPKAPGTLVSNDDDKTGLQVKAISNLDNQTMFNVSGPGMKGMVGMASRTLAAISRSGISVSLITQSSSEYSISFCVATSDAHKAKCALEQEFELEIKSELLEPIELREGLAIVSLIGDGMKTHKGVAAKFFSALAKASVNIVAIAQGSSERSISTVIQQSKTKHAIGACHQAFFDVQQYVDVFLVGAGNVGAGLLEQIKQQNEMLKELHISIRVCGIANSRQMLLDSNGIDLANWQSLLAQQGQAYDLAGLLLWGQEQQLLNPVLVDCTSSEQVSDGYLDVMNAGFHVVTPNKKANTRDYDYYQALRQVSLSQRRQFLYETTVGAGLPVIDNLKKLLCAGDKLHKFNGVLSGSLSFIFGMLDEGMSLSQATTIARDKCFTEPDPRDDLSGMDVARKVLILAREVGLQLELSDINIESVLPADFDDSGDVNTFMANLPSLDAQVSQRVANAKAQGKVLRYVGQIDDGKCHVRVVEVDAYDPV",
    "response#3": "MRVLKFGGTSLANAERFARAADIIESNAQQGQVAAVLSAPAKITNHLVAMIDKTVAGQDIQPNLNDAEQIFADLVQGLHAELPAFALESVKSMLAREFANLRQLLHGISLLGQCPDSINASIISRGEKMSIAIMDALFVARGYPVKVLDPVEKLLARGGYLESTVDIEVSTARFQADPIPQGAVALMAGFTASNEKGELVVLGRNGSDYSAAVLAACLRAECCEIWTDVDGVYTCDPRLVPDARLLKSMSYPEAMELSYFGAKVLHPRTILPIAQFQIPCLIKNTANPQAEGTLIGAESGAEQLEVKGITNLSNVAMLNVSGPGMKGMVGMAARVFSVMSRAGISVVLITQSSSEYSISFCVPQAELAKARRALNDEFYLELKNGLLEPLDVQEKLAVVSVVGDGMRTARGISARFFTALAQANINIVAIAQGSSERSISAVIADEKVTEAVRTCHEALFNSRQVLDVFIIGVGGVGGALVEQIRRQQPRLLAQNIQLRVCGLSNSRSMLLDVNGIELEHWRDQISAANEPYSFARLIRVVNQYHLLNPVIVDCTSDQKIADQYADFLAAGFHVITPNKKANTSSLDYYRLLRRTAQSSRRKFLYETNVGAGLPVIENLQNLLNAGDELSRFSGVLSGSLSFIFGKLEEGMSLSAATALARQNGYTEPDPRDDLSGMDVARKLLILAREAGYPLELSDIQVQGVLPASFDASGDTASFMARLPQLDAEFAARVAAANSEGKVLRYVGVIEEGQCRAEIQAVGADDPE"
  },
  {
    "reference": "MKTLKDILYGVSLTELHGDREREISSLAFDSRRVKKGGLFIAIKGLNVDGHDYIETAIDLGAVAILCESLPEKRKKSITYVQVDNSAAAMGIVAANFHDNPSRKLNLVGVTGTNGKTTTATLLFQLFQQLGYKVGLLSTVENKIDHKVLDAQFTTPDSLQLNELLAQMVSQGCTHCFMEVSSHALVQHRTSGLQFAGGIFTNISHDHLDYHETFEAYIKAKKMLFDKLPKEAFALVNADDKRGMVMLQNTKAEKNTYSLKSMADFRAKILHNTLQGLELDVDGISAWFKLIGDFNAYNLLAVYAAGVLLEEEKEEVLTALSQIDGARGRFEQVYNGNNILAIVDYAHTPDALDNVLRTIDKLRTRQEKVITVIGCGGNRDKTKRPEMARIAAEFSNKVILTSDNPRDEDPAVILEEMMKGVPKVAQKNTMIIQDRKEAIRTACNLAEKMDIILVAGKGHETYQEIKGVRNHFDDKEILNEMLNGQ",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should interact with enzymes and substrates within the Cell wall biogenesis pathway to enhance or regulate its activity.2. The designed protein have Mur ligase N-terminal catalytic domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "VTGTEGKTSVTQFIVKAFEALNIDCGLIGTNGIGFLHNLKENTHTTPDLLALYQSLAEIDQNDNVRVLIDGRLPIALEVTSHALDQKRVEGLTFETTVFTNLNRDHLDYHGTLEAYGAAKARLFLEYPQKNSVINLDDPFGATLYQRLKQERPEVRLFPFGTKPVDDPRYLHIENLELHAEGLRFTLNFEGAAYPIKSPLYGAFNAYNLAAAIAVTGTNGKTTSLSLLSQAMTLLDRPCGQIGTLGSGFWGALHGGGLTTPDVVSLHRELAVLLEAGAQCACVEVSSHALDQGRVDEVKFRAALFTNISHDHLDYHGSMEAYVKAKKSLFSRVGLEHVVVNLDDRYADEFLAEVDDQVALMTYGSEGDVRVVEAKPFESGLLVRLATPYGEMAFTSPLVGMINLPNLVGVAAG",
    "response#2": "VTGTNGKTTISQLVAQLTQLTGMSSAVMGTAGNGRLGALVQASHTTGDSLAVQQFLYQMGIEKAELLALEASSHGLDQQRLQGMPVSVAIYTNLSRDHLDYHADMAEYAAAKAKLFDKAHFPELTHAIVNIDDEHAQIMLDTAHASQLTVWTYSLDPAKSATFVAAEIKPSLQGVEIVLRTDFENKEINSINIVSPLLGRFNVANLLAAIAAAR",
    "response#3": "VTGTNGKTSCSQWLGNALSRLGQPTAVIGTLGVGIFKHGEHGTFNVTGYTTPDAVLLQRELVNMHHAGATALAIEASSIGLHQGRLYGVHFDMALFTNFTRDHLDYHGDMAAYEAAKTMLFDWPGLRHAVVNLDDALGVRLVERLKRRQSGIGITGYTLTNQGVADIDLLSASDIRSTHAGTSFQLTSKFGVTLVKTQLVGEFNVSNVLGIIA"
  },
  {
    "reference": "MSVQANAPAAVIVLAAGAGTRMKSAKPKILHELGGRSMVGHALAAAEGLAPQTLVAVVRFERERVVEHLLQIKPDLVIADQDEVPGTGRAVQQGLDQLDPALEGTIVVTYGDVPLLTTELLNELVATHDREGNAVTVLTALLQDATGYGRILRAEDGTVTGIREHKDATDEERAIREVNSGIYAFDAQVLRSALAEVTTDNAQGEMYLTDVLGIARSTGGRVAAVVTEDAWQVEGANDRVQLAALGKEMNRRILEKWMRSGVSIIDPDSTWIDVQVALSNDVTIKPGTQLHGTTAVGTDAVIGPDTTLTNTQVGRGAKVVRSDVTDSSIAEGSSVGPFAYLRPKTILGPDAKIGAFYETKNIVVGRGSKLSHLGYAGDAEIGEFTNIGCGNITANYDGVNKHRTVIGSHVRTASNTVFVAPVSVGDGAYTGAGAIVRKNVPAGALALTVAPQRNTEGWTITKRPGSPAADAAFKSALDSGLAAGSADQAK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. We need a protein that can perform acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate with high efficiency.2. The protein requires Mg(2+) to activate a critical catalytic site.3. The protein should have a high affinity for Mg(2+), acetyl-CoA.4. The designed protein should have magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity within cytoplasm to regulate cell wall organization, regulation of cell shape.5. A protein that is capable of catalyzing reactions within the Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVDLARPQGFGRILRDGNGNVIGIVEQKDASDLQKKINEINTGILAVSAKSLRQWLAKLDNNNAQGEYYLTDIVAMSVADGVKIATAQPADEWETLGVNSKVQLAELERIHQRNIAEQLMVQGVTLVDPRRIDVRGKLACGRDVLIDINAVFEGEVELGDGVQIGANAVIRDTSIGAGTVIHPHCVLEQSRIGANCQIGPYARTRPGVEMADDAKLGNFVEIKKSRIGKGSKINHLSYIGDAIIGANVNVGAGTITCNYDGANKHRTTIGDGAFIGSGVELVAPVEIGAGATVGAGSTITKAVPADELVLERSQQRNIANWKRPVKKK",
    "response#2": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLTAKVSDPTGYGRIIRDENGELVAIVEHKDANPEQLAITEVNTGVMAIKGAYLHQSLGQLGNDNSQNEYYLTDIVKMAVKDGLSISATEATDETEVLGVNDRVQLAELERIFQKNQARQLMISGVSLINPANTYIGVDVKIGSDTVIQPGVMLEGNTVIGEDCHIGPNSRITNSTIGDRTKVDSSVVDSSVVGSDVAVGPFAHIRPATNVGNRAKVGNFVELKKTTLGEGSKASHLTYLGDAEIGTDVNIGCGTITVNYDGVNKFKTIIGDRSFIGCNANLVAPVTINSDTYVAAGTTITKEVPPFALAIGRSRQRNIEGWKRPVKK",
    "response#3": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVAIGAEPEDPGRYGRLIMEDGTLERIVEFKEATEAERAIAFCNSGILCASAKTLFELIDAVGNDNAAGEYYLTDIVALARARGHKATVVRCPEAETLGINTRGELARAEALFQDVRREDFMKAGVTLRDPKTVWFSWDTEIGKDVTIFPNVVFGPGVSIADKVEIRAFCDIEDAVIGEGASIGPFARIRAGAELGRDVHLGNFVEVKKSRIGDGTKAGHLSYLGDAEIGTKTNIGAGTITCNYDGVNKDKTIIGNNVFIGSNASLVAPVSIGDGAYTASGSVITEDVPADALALGRARQTAKEGRAAELMRAARARKAARKAAK"
  },
  {
    "reference": "MEEIKKYLKLEISQQRNFIYPLIFQEYIYAFAYDRGFSRSILILSENLGYDNKSSLLLVKHLITRIYQHNYFIIAPDDSNPNPFWVCSKNFFYEIILEGFAFIVEIPFSLKLISCLEEKNKKIVKYQNLRSVHSIFPFLEDNFSHLNCLFDILIPHFVHTEILVQTFRYCITDVSFLHLLRFFLYKYCKWNSLMTPKKASYSFSKSNQRLLLLLFNIHVYEYESFFVFLHNHSSHLRSIHFEVLLERIHFYGKMECLINIFIKLKDFHANLWLVNESCIHYIRYQRKLIMASKGTSFFMNKWKFYFITFWQCYFSLWFYTRRIYINQSSNHSFEFFGYLSSLRMNPSVVRSQIIENSFLINNIIKKVDTLVSIIPLISSLAKAKFCNVLGHPTSKPIWADLSDYNIIDRFGCICRNLFHYHSGSSKKKFLYRIKYILRLSCARTLARKHKTTVRTFFKIFGSELLEEFLMPEEYVFFFTLSKASCSLQGVYISRIWYLDIISINNLTNYK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in tRNA processing, potentially with improved properties such as stability, specificity or selectivity.2. For general function, the protein need meet that Probably assists in splicing its own and other chloroplast group II introns. The designed protein sequence is ",
    "response#1": "KXFDTRVPVISFIGSLAKAKFCTVSGHPISKPIWTDLSDCDIIDRFGRICRNLSNYLSGSSKKQSLYRIKYILRFSCARTLARKHKSMVRAFLQRLGSGLLEEFFTEEEQVVSLIFPKAL",
    "response#2": "MKKLDTRVPIILLIRSLVKAKFCNVLGHPLSKSVWADLSDFDIIDRFLRICRNFSHYYNGSSKKKNLYRIKYILRLSCIKTLARKHKSTVRAFLKRLDSEKLLEEFFTEEEDIFSLIFPRTY",
    "response#3": "KXFDTRVPVISFIGSLAKAKFCTVSGHPISKPIWTDLSDCDIIDRFGRICRNLSNYLSGSSKKQSLYRIKYILRFSCARTLARKHKSMVRAFLQRLGSGLLEEFFTEEEQVVSLIFPKAH"
  },
  {
    "reference": "MKNKIHRALNIKRDKKRTVQLMSDKKNMKLFSLNSNHSIAEKIAEAAGVPLGKLSSRQFSDGEIQINIEESVRGYDIYIIQSTSFPVNNHLMELLIMVDACKRASANTINVVMPYFGYARQDRTAAPREPITAKLVANMLVGAGVDRVISLDLHAVQVQGFFDIPVDNLFTIPLFAKHYCNKGLTGEDVVVVSPKNSGVKRARNLAEYLDAPIAIIDYGEDEAGRSEGYIIGDVEGKKAILIDDILNTGRTFSEAAKIVCREGAVEIYAVSSHGLFADKAAELLDDSPIREILVTDSVDTKEKKPKNLHYITASELIGDAIVRIQERRPVSPLFAYQKK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The presence of Ribose-phosphate pyrophosphokinase N-terminal domains is critical for the interaction of the designed protein with its intended targets.2. The protein must exhibit the following characteristics: Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P)..3. The designed protein should be involved in ribonucleoside monophosphate biosynthetic process, nucleotide biosynthetic process, 5-phosphoribose 1-diphosphate biosynthetic process, phosphorylation by localizing to cytoplasm and exhibiting ribose phosphate diphosphokinase activity, ATP binding, kinase activity, magnesium ion binding.4. The designed protein should have a high specificity for Mg(2+), ATP, D-ribose 5-phosphate binding, with minimal off-target effects.5. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.6. The protein have enhanced ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-   diphosphate + AMP + H(+). The designed protein sequence is ",
    "response#1": "MKIFSGRSNLALSQKVSDYLNMPLGQVKIQPFPDGELLIKLEEDVRGRDTFIIQPTCGNVNESLVELLVFIDCARRASAERITAVIPYFGYARQDRKDEGRVPITAKLVANLIATAKRFGTTPRVVAVDLHAAQIQGFFNIPVSPTLVDYIASKQLEDLVVVSPDVGGVARARALAKRLNDAPLAIIDKRRQAHNVAEVMNVIGDVRGKTAVLVDDMIDTAGTILEGAKILRKEGARQVYACATHAVFSPPAIERLSSGYLEEVIVTNSVPLSDAAKKEGITVLSVAGLLGEAIRRTHTGQSITSMFDGAA",
    "response#2": "MKIFSGRSNLALSQKVSDYLNMPLGQVKIQPFPDGELLIKLEEDVRGRDTFIIQPTCGNVNESLVELLVFIDCARRASAERITAVIPYFGYARQDRKDEGRVPITAKLVANLIATAETFYGQPSRKLKMIGSATPLLARHFRREGLDGAVAVSPDAGGVPRAEDFRARVGGSLAIISKRHPGPDMAEALEMVGDVEGKVAVIVDDMISTGGTLTEATELLRERGAARIYATATHGIFAADALASPTLVDYIASKQLEDLVVVSPDVGGVARARALAKRLNDAPLAIIDKRRQAHNVAEVMNVIGDVRGKTAVLVDDMIDTAGTILEGAKILRKEGARQVYACATHAVFSPPAIERLSSGYLEEVIVTNTIPLPPDQRLESPTLVDYIASKQLEDLVVVSPDVGGVARARALAKRLNDAPLAIIDKRRQAHNVAEVMNVIGDVRGKTAVLVDDMIDTAGTILEGAKILRKEGARQVYACATHAVFSPPAIERLSSGYLEEVIVTNTLPIPPERKLENPQLKLLTVTDILAEAIESVFEDGR",
    "response#3": "MKIFSGRSNLALSQKVSDYLNMPLGQVKIQPFPDGELLIKLEEDVRGRDTFIIQPTCGNVNESLVELLVFIDCARRASAERITAVIPYFGYARQDRKDEGRVPITAKLVANLIATAEAFFGKQPETMVAMDLHAAQIQGFFDGPVSPTLVDYIASKQLEDLVVVSPDVGGVARARALAKRLNDAPLAIIDKRRQAHNVAEVMNVIGDVRGKTAVLVDDMIDTAGTILEGAKILRKEGARQVYACATHAVFSPPAIERLSSGYLEEVIVTNTLPLPPEEKFDGVKIFSGSSNPKLAQHICDELGLPLGKIKLSRFKSGEIYCLYEESVRNCDVFIVQTFSHPINEHLVETLIMMDAAKRASARTINLVLPYYGYSRQERKTAPREPISAKLLADLLHTAALPVMVDYIRSLNIPNLIVVSPDVGGVKMGSAFASALGTRLAIVVKQRKSATETEASHIIGDVAGKNILIIDDLTETAGTITSAAKILRKHGALDIYAGVSHAVLTDLAVERLKDSDIKELIFTNTIPLPSEKRFDGFSLYMAKAIINGRGDEVIELAKTNLF"
  },
  {
    "reference": "MDRKRLLKNPLLWIVAVLLLYFVFSTIFDSDRGYTHVPTSRAVEQIASGNITEANLEDKEQQLKLHLANPIDVDGQQVEQIITKFPAGASDQLYNSLINAEHNGNPVEFDTTVTQDSFFTQLLIYMIPLGILLLLLMWMMNNAQGGGNRVLNFGKSKAKQLNKDMPKTTFGDVAGADEAVEELHEIKDFLQNPARYQALGAKIPKGVLLYGPPGTGKTLLARAVAGEAGVPFYTISGSDFVEMFVGVGASRVRDLFEQAKQNAPCIIFVDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFDSRGGIILIAATNRPDILDPALLRPGRFDRQIPVSAPDLRGRRAILEVHSKGKPIAENADLEALAKRTVGMSGADLANVINEAALLTARTNGHVITDSALEESVDRVIGGPARKSRIISEKEKKITAYHEGGHALAAWAMPDIEPVYKLTILPRGRTGGHALLVPEDDKELMTRSEMIGRLVFAMGGRTAEELVFHEPTTGAVSDIEQATKIARSMVTEYGMSPRLGAVKYGQEQGDPFLGRSAGRQPDYSLEVAHEIDEEVRKLIETAHTEAWEVLNTYRDVLDDLVVELLEKETLQRRDLERIFAAVEKRPRITAFNEFGERLPSDKPPIKTPAELAMERGEPWPPEPEEPERDRREEERQPEPTPVGTAPGAHDVPGGPPRSQPEPPPNPYAPPPSSGNPNGNRRNNGGQQGGGQGSWPPPYAGGQQGGGYPSPSGGQSGPPNYGAPPGWTPATTPGNRPS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should exhibit Polar residues, Basic and acidic residues compositional bias.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The designed protein should contain one or more AAA+ ATPase domains that are essential for its function.4. The protein should have a high affinity for ATP, Zn(2+).5. The protein must exhibit the following characteristics: Plays a role in the quality control of integral membrane proteins.; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins.6. A protein with ATP binding, ATP-dependent peptidase activity, metalloendopeptidase activity, zinc ion binding is needed to enhance cell division, proteolysis in a specific context. The designed protein sequence is ",
    "response#1": "VLLLGAPGTGKTLLAKAVAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFNKARKNAPCIIFIDEIDAVGRKRGAGQGGGNDEREQTLNQLLVEMDGFNSEETIIVLAATNRPEILDKALMRPGRFDRQVVVQYVGLTEVNPQTIARGTPGFSGADLANLVNEAALFAARANKRVVTMDDFRXAXAXVX",
    "response#2": "MPHGILLEGPPGTGKTMLARALAGEAGVPFFSISASEFIEVFVGVGASRVRKLFEEAKKAAPSIIFIDELDSVGRVRGAGLGGGHDEREQTLNQILAEMDGFEGHEAVVVLAATNRPDVLDPALLRPGRFDRHVTLELPSAEDRLGILRIHAEAEGKTFDEDVDLHTIARKTPGFVGADLANMLNEAAILAARSGRETITMEDLESGINRLIPKGVLLVGPPGTGKTLLARAIAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLNQLLVEMDGFEPNESIILIAATNRPDVLDPALLRPGRFDRQVVVPNPTVEVAYTHAIKGVADDVDLHTIARKTPGFVGADLANMLNEAAILAARSGRETITMEDLEQGVNRAIYSTRVGAEPQAAEKK",
    "response#3": "MLLVGPPGTGKTLLARAVAGEAGVPFLTVSASEFMEMFVGVGASRVRNLFEEARKASPAIIFIDELDSIGRRRGAGIGGGHDEREQTLNQILSEMDGFEKDTSVIIIAATNRPDILDPALLRPGRFDRQVTIHLDALTENHRLGILRKKAEEGGVNIPQDVLDMLVSTVETNIRELEGALIRVTAFASLNQQTIDLELAKKALRDLLKTEEKQVRVIVEKEKLIVAYHEIGHALVQWVLPYTEPVHKVTTIPRGMAALGYTMTLPTEDRYLKSKNEYLSEIRTLLGGRASEEVVFGDITTGASNDIERATAIAHAMVTKFGMSEKFGPILLDNTNDGDLFMQKHYSETTGKEVDDEVRTLITEAYEDSKKILRDNYEKLEKVTRALLDRETISGIEGMIADLQRRM"
  },
  {
    "reference": "MTSAPPRWTTAELAEDAATSAAQFRTERLAVTDSWGTHYNQARGKFELLFKKLSDLNPEAITDDNLAEAYGLGLGEALRYLAGPPISDDDLQVIADVESIAPGVLKKNAEALRKVFGVIERVIDPHRFPWMETGGMPTEQQREAALLASSVLLAAQRIATERRNEGKDNQETMVKDYLRTLGFTEAPAAAINTIVKGPQAMQFCAECQLGERKADVVVRLHDTRLMAIECKVSNSSTNSVKRLNNDAAVKAEYWIKQFGTAQVVPSRSLVSSRY",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must be able to DNA binding, type II site-specific deoxyribonuclease activity.2. The protein have enhanced Endonucleolytic cleavage of DNA to give specific double-   stranded fragments with terminal 5'-phosphates..3. The protein must exhibit the following characteristics: A P subtype restriction enzyme that recognizes the double- stranded sequence 5'-GTCGAC-3' and cleaves after G-1.. The designed protein sequence is ",
    "response#1": "FMSYNQNKQYLEDNPEIQEKIELYGLNLLNEVISDNEEEIRADYNEANFLHPFWMNYPPLDRGKMPKGDQIPWIEVGEKAVGSKLTRLVSQREDITVREIGLPTGPDERYLLTSPTIYSLTNGFTDSIMMFVDIKSVGPRDSDYDLVLSPNQVSGNGDWAQLEGGIQNNQQTIQGPRSSQIFLPTIPPLYILSDGTIAPVVHLFIKPIYAMRSLTKGDTGQSLYKIKLASVPNGLGLFCNPGYAFDSAYKFLFRPGKDDRTKSLLQKRVRVDLRVLDKI",
    "response#2": "FMSYNQNKQYLEDNPEIQEKIELYGLNLLNEVISDNEEEIRADYNEANFLHPFWMNYPPLDRGKMPKGDQIPWIEVGEKAVGSKLTRLVSQREDITVREIGLPTGPDERYLLTSPTIYSLTNGFTDSIMMFVDIKSVGPRDSDYDLVLSPNQVSGNGDWAQLEGGIQNNQQTIQGPRSSQIFLPTIPPLYILSDGTIAPVVHLFIKPIYAMRSLTKGDTGQSLYKIKLASVPNGLGLFCNPGYAFDSAYKFLFRPGKDDRTKSLLQKRVRVDLRVLDKL",
    "response#3": "FMSYNQNKQYLEDNPEIQEKIELYGLNLLNEVISDNEEEIRADYNEANFLHPFWMNYPPLDRGKMPKGDQIPWIEVGEKAVGSKLTRLVSQREDITVREIGLPTGPDERYLLTSPTIYSLTNGFTDSIMMFVDIKSVGPRDSDYDLVLSPNQVSGNGDWAQLEGGIQNNQQTIQGPRSSQIFLPTIPPLYILSDGTIAPVVHLFIKPIYAMRSLTKGDTGQSLYKIKLASVPNGLGLFCNPGYAFDSAYKFLFRPGKDDRTKSLLQKRVRVDLRVLDKG"
  },
  {
    "reference": "MVTPGNIGSFTVWDYVVFALMLLISAVIGIYYAFAGGGQKTSKDFLMGGRSMTAVPVALSLTASFMSAVTVLGTPAEVYRFGSMFSIFAFTYAIVVVISSEVFLPVFYRLGITSTYEYLELRFNKFVRLLGTILFIIQTVLYTGIVIYAPALALNQVTGFDLWGAVVATGVVCTFYCTMGGLKAVVWTDVFQVGIMVAGFSSVIIRAVVVQGGIGPILNDSYYGDRLNFWDFTPNPLQRHSFWTIVVGGTFTWTGIYGVNQSQVQRYIACKTRFQAKLSLYINLLGLWAILACAVLSGLAMYSIYKDCDPWTAQFVSAPDQLMPYLSLDILRDYPGLPGLFVSCAYSGTLSTVSSSINALAAVTVEDLIKPYFRSLSETKMSWISKGTSLIYGAICIAMAGLASLMGGLLQAALSIFGMVGGPLLGLFALGIIFPFVNSLGAVIGLLSGFAISLWVGIGSQIYPPTASSSLPKPLSLEGCNFTSFESNWTTTVMPMMTTLIPEVSSRPELADSWYSLSYLYFSTLGTIVAVVVGVIASLLSGGLKQNVNRDFLLTSQDFSYLNVLFSNCKKKGQEEKVEVLNWKMRSTDTDMDQGTDNPAFNHMEMSSTEKKEKMNGIIA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Catalyzes passive carrier mediated diffusion of iodide; Acts as an electrogenic sodium (Na(+)) and chloride (Cl-)- dependent sodium-coupled solute transporter, including transport of monocarboxylates (short-chain fatty acids including L-lactate, D- lactate, pyruvate, acetate, propionate, valerate and butyrate), lactate, mocarboxylate drugs (nicotinate, benzoate, salicylate and 5- aminosalicylate) and ketone bodies (beta-D-hydroxybutyrate, acetoacetate and alpha-ketoisocaproate), with a Na(+):substrate stoichiometry of between 4:1 and 2:12. The Extracellular topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.3. The protein should be designed to localize specifically to membrane in order to perform its role in sodium ion transport more effectively. The designed protein sequence is ",
    "response#1": "IGSFVVWDYVVFAGMLLISAAIGIYYAFAGGGQQTSKDFLMGGRSMSAVPVALSLTASFMSAVTVLGTPAEVYRFGAIFSIFVITYFFVVVISAEVFLPVFYRLGITSTYEYLELRFNRCIRLCGTILFIVQTILYTGIVIYAPALALNQVTGFDLWGAVVATGVVCTFYCTLGGLKAVVWTDVFQVGIMVAGFASVIIQASITQHGINKILSDAFNGGRLNFWNFDPNPLQRHTFWTIVIGGTFTWTTIYGVNQSQVQRYISCKSRLHAKLSLYVNLVGLWVILTCSIFCGLALYSRYRECDPWTSKKVSAIDQLMPYLVLDILKNYPGVPGLFVACAYSGTLSTVSSSINALAAVTVEDLIKPRFKSLSEKSLSWISQGMSVLYGALCIGMAALASLMGALLQAALSIFGMVGGPLLGLFSLGILVPFANSIGALTGLLAGFAISLWVGIGAQLYPPLPERTLPLPLETYGCNITHNGSDWMSTTEMPFSTSAFQIHNAERTPLMDNWYSLSYLYFSTIGTLTTLFVGILISLSTGGRKQIGSFTVWDYVVFALMLLISAVIGIYYAFAGGGQKTSKDFLMGGRSMTAVPVALSLTASFMSAVTVLGTPAEVYRFGSMFSIFAFTYAIVVVISSEVFLPVFYRLGITSTYEYLELRFNKFVRLLGTILFIIQTVLYTGIVIYAPALALNQVTGFDLWGAVVATGVVCTFYCTMGGLKAVVWTDVFQVGIMVAGFSSVIIRAVVVQGGIGPILNDSYYGDRLNFWDFTPNPLQRHSFWTIVVGGTFTWTGIYGVNQSQVQRYIACKTRFQAKLSLYINLLGLWAILACAVLSGLAMYSIYKDCDPWTAQFVSAPDQLMPYLSLDILRDYPGLPGLFVSCAYSGTLSTVSSSINALAAVTVEDLIKPYFRSLSETKMSWISKGTSLIYGAICIAMAGLASLMGGLLQAALSIFGMVGGPLLGLFALGIIFPFVNSLGAVIGLLSGFAISLWVGIGSQIYPPTASSSLPKPLSLEGCNFTSFESNWTTTVMPMMTTLIPEVSSRPELADSWYSLSYLYFSTLGTIVAVVVGVIASLLSGGLKQ",
    "response#2": "IGSFTVWDYVVFALMLLISAVIGIYYAFAGGGQKTSKDFLMGGRSMTAVPVALSLTASFMSAVTVLGTPAEVYRFGSMFSIFAFTYAIVVVISSEVFLPVFYRLGITSTYEYLELRFNKFVRLLGTILFIIQTVLYTGIVIYAPALALNQVTGFDLWGAVVATGVVCTFYCTMGGLKAVVWTDVFQVGIMVAGFSSVIIRAVVVQGGIGPILNDSYYGDRLNFWDFTPNPLQRHSFWTIVVGGTFTWTGIYGVNQSQVQRYIACKTRFQAKLSLYINLLGLWAILACAVLSGLAMYSIYKDCDPWTAQFVSAPDQLMPYLSLDILRDYPGLPGLFVSCAYSGTLSTVSSSINALAAVTVEDLIKPYFRSLSETKMSWISKGTSLIYGAICIAMAGLASLMGGLLQAALSIFGMVGGPLLGLFALGIIFPFVNSLGAVIGLLSGFAISLWVGIGSQIYPPTASSSLPKPLSLEGCNFTSFESNWTTTVMPMMTTLIPEVSSRPELADSWYSLSYLYFSTLGTIVAVVVGVIASLLSGGLKQIGSFTVWDYVVFALMLLISAVIGIYYAFAGGGQKTSKDFLMGGRSMTAVPVALSLTASFMSAVTVLGTPAEVYRFGSMFSIFAFTYAIVVVISSEVFLPVFYRLGITSTYEYLELRFNKFVRLLGTILFIIQTVLYTGIVIYAPALALNQVTGFDLWGAVVATGVVCTFYCTMGGLKAVVWTDVFQVGIMVAGFSSVIIRAVVVQGGIGPILNDSYYGDRLNFWDFTPNPLQRHSFWTIVVGGTFTWTGIYGVNQSQVQRYIACKTRFQAKLSLYINLLGLWAILACAVLSGLAMYSIYKDCDPWTAQFVSAPDQLMPYLSLDILRDYPGLPGLFVSCAYSGTLSTVSSSINALAAVTVEDLIKPYFRSLSETKMSWISKGTSLIYGAICIAMAGLASLMGGLLQAALSIFGMVGGPLLGLFALGIIFPFVNSLGAVIGLLSGFAISLWVGIGSQIYPPTASSSLPKPLSLEGCNFTSFESNWTTTVMPMMTTLIPEVSSRPELADSWYSLSYLYFSTLGTIVAVVVGVIASLLSGGLKQ",
    "response#3": "LLGDLLSGLSVAIMQLPQGLAYALLAGLPPVFGLYSSFYPVFIYFLFGTSRHISVGTFAVMSVMVGSVTESLAPQALNDSMINETARDAARVQVASTLSVLVGLFQVGLGLIHFGFVVTYLSEPLVRGYTTAAAVQVFVSQLKYVFGLHLSSHSGPLSLIYTVLEVCWKLPQSKVGTVVTAAVAGVVLVVVKLLNDKLQQQLPMPIPGELLTLIGATGISYGMGLKHRFEVDVVGNIPAGLVPPVAPNTQLFSKLVGSAFTIAVVGFAIAISLGKIFALRHGYRVDSNQELVALGLSNLIGGIFQCFPVSCSMSRSLVQESTGGNSQVAGAISSLFILLIIVKLGELFHDLPKAVLAAIIIVNLKGMLRQLSDMRSLWKANRADLLIWLVIGSFVVWDYVVFAGMLLISAAIGIYYAFAGGGQQTSKDFLMGGRSMSAVPVALSLTASFMSAVTVLGTPAEVYRFGAIFSIFVITYFFVVVISAEVFLPVFYRLGITSTYEYLELRFNRCIRLCGTILFIVQTILYTGIVIYAPALALNQVTGFDLWGAVVATGVVCTFYCTLGGLKAVVWTDVFQVGIMVAGFASVIIQASITQHGINKILSDAFNGGRLNFWNFDPNPLQRHTFWTIVIGGTFTWTTIYGVNQSQVQRYISCKSRLHAKLSLYVNLVGLWVILTCSIFCGLALYSRYRECDPWTSKKVSAIDQLMPYLVLDILKNYPGVPGLFVACAYSGTLSTVSSSINALAAVTVEDLIKPRFKSLSEKSLSWISQGMSVLYGALCIGMAALASLMGALLQAALSIFGMVGGPLLGLFSLGILVPFANSIGALTGLLAGFAISLWVGIGAQLYPPLPERTLPLPLETYGCNITHNGSDWMSTTEMPFSTSAFQIHNAERTPLMDNWYSLSYLYFSTIGTLTTLFVGILISLSTGGRKQ"
  },
  {
    "reference": "MSDMKIFLASKNKDKITEINKILSKNNIDLLTCHDINIPDVEENGSTFVENALLKAKSASKIAKLPTIADDSGIEVDYLNGRPGIISARYSGTNATNENNNLKLLEELKDVPFEQRKACYRCVIVFMRFPEDPFPFISTGSWSGYITDKPKGENGFGYDPIFYLPDYKKTSAEISPEEKNKISHRSIALKKFEEFIYKKNII",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP2. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.3. The substrate binding site should be located in a region of the protein that is accessible to the ligand.4. A protein with metal ion binding, XTP diphosphatase activity, ribonucleoside triphosphate phosphatase activity, dITP diphosphatase activity, ITP diphosphatase activity is needed to enhance purine nucleoside triphosphate catabolic process, nucleotide metabolic process in a specific context.5. The protein should have a fast turnover rate for dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MKVLILASGNAGKLREIQDLLGSLGISVTPQSAFGVTEAEETGLTFVENAILKARHAARITGLPALADDSGLAVDALGGAPGIYSARYAGEGASDAANNAKLLSELGGVADAQRSARFCCVLVLMRHAEDPLPIVCHGQWHGRVLHAPRGTGGFGYDPLFWVPALGCSSAELPAEQKNRISHRARAMTALLPLLKAQLDS",
    "response#2": "MKILFLGTSGYIPTKERNVTSIALQLENEIWFCLDCGEGSQRALVNYRLNPLRIKNIFITHLHGDHIFGLPGLLQTMSLFGREEPLYIYGPAGLKKFVEATLKLCHTRMRYPLQIREIDEGKIFSRDGFSVETFPVNHRIPTIGYIFIEKPRPGILNIEKAKKLGVPPGPLWGKLQRGEDVSLQDGRVIKGEDFCSPPSKGRSFAYCSDTVFNENIVDLAQDADLLVHESTFCSSMEEIAREKGHSTSMQAAKVAKEACVKKLLLTHISSRYRDEHELLKEAQEIFPNTYLAKDLLTIPI",
    "response#3": "MNNEKLVIASNNAGKLREISALLAPYGIEIIPQSAFNVSEAEEPHVTFIENALAKARHASRISGLPALADDSGLCVDALNGAPGIYSARYAGEHGNDAANNAKLLTELAGKPNRSAHYYCVLVLMRHADDPTPLIACGEWHGEILQTARGENGFGYDPLFWLPEHGCSAAQLPAEKKNQLSHRAKALQQLISLLQLRKD"
  },
  {
    "reference": "MSEKQPTPDSDKSRRSRSFGSFLIVLVVLLLVLAVVGNDPFASKLELSQDQYEWYLNKGDVLVQEYKGSEAGTNLIKGRLRSPDKGEVTFEVRYASLKEREERFRDLKSRSYVTTRAAAFRHDVEQGRYVPIEARSLTANTTRRSKVPGEKGGAEKGTLVVDPEPESRLLVKATATPTSSVTREGGERLVPPPLRLGHVDLDVRLEPGDDLAALERMLEERGVPIERRTFDLSLEDAGGTRWDSNTSPFSSILFLLGPWVLLFVVFLIFMRQMRSQGGAGGVMSFGRSRAQLYSKESHTNTTFDDVAGADEAKDEVRELVEFLKNPGRFTRIGGRIPRGVLLVGPPGCGKTLLAKAIAGEAEVPFFSISGSDFVEMFVGVGASRVRDLFKQARENSPCIIFLDEIDAVGRRRGSGMGGGHDEREQTLNAILVEMDGFGTDEGIIVVAATNRPDVLDPALLRPGRFDREVTIDLPDMEGRRAILGVHLKRVKSGPDADVSGLARSTPGYSGADLAAIINEAAIMAVLAKRDMVSMADLEEARDKVRYGRQKRSRKIEEQDRRITAYHEAGHAVVAAALPETDSPHKVTIVPRGRALGATMIVPERESYHMQRAKLHAQIAMLFGGRVAEALYCGDISAGASDDIRRATDLARAMVAELGMSEKIGPINYAERQGSDFLGTEIGRGKIHSEETAREIDQEVMRFLTEAYQRAESILRANGRAVEEIAQALLLYETVNGEEVARILAGASAKGLRPEPDAEQKDAPKKSGT",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must have a active site that is structurally stable and resistant to denaturation.2. The protein design must be able to perform zinc ion binding, ATP binding, ATP hydrolysis activity, metalloendopeptidase activity in order to contribute to protein catabolic process.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "VLLVGPPGCGKTLLAKAIAGQAEVPFFSISGSDFVEMFVGVGASRVRDLFKQAKDNSPCIIFLDEIDAVGRRRGGGFTTGGHDEREQTLNAILVEMDGFEANDQIIVIAATNRSDILDPALTRPGRFDRQVLVPYVE",
    "response#2": "IPRGVLLLGSPGTGKTLLAKAIAGEANVPFFSISGSDFVEMFVGVGAARVRDMFEQAKKQSPCIVFIDEIDAVGRQRGAGLGGGNDEREQTLNALLVEMDGFEGASGVIVIAATNRPDVLDAALLRPGRFDRQVVVPLPLNFMDGVSLLARADEMGIPKDRILVLLAATNRPEILDPALLGYAKQR",
    "response#3": "VPKGILLVGPPGTGKTLLARAVAGEAKVPFISTNGSEFVEMFVGVGAARVRDLFTLARARTPAIIFIDELDALGRARGSMPMLGGHDEKEQTLNQLLAELDGFDPSAGLVLLAATNRPEILDPALLRAGRFDRQILVDRPTMEQDVNLHTIAASTVGFTGADLENLANEAALLAAKKNLRAITMTEIEYARGEV"
  },
  {
    "reference": "MINHNIISFKKFEAIFEQRPGGLELNMPIEIGSNNVDLTSKPFNIKGGSHYKVKIGYMASEPITDLICQIHTFRKSTPYGRDTQKVGDICANTNDLEFDFPKFGWEQASTQSSSMGDYTIKMYFKGNGNQDLATFFYSFNVAPDWENALPIN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.2. The designed protein should have Rho GDP-dissociation inhibitor activity, GTPase activator activity within cytosol to regulate Rho protein signal transduction, regulation of Rho protein signal transduction. The designed protein sequence is ",
    "response#1": "IKCVTVGDGAVGKTCMLISYTSNTFPTDYVPTVFDNFSANVVVDGSTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLLSRASYENISKKWIPELRHYAPTVPIVLVGTKLDLREDRQYLIDHPGATPITTAQGEELKKAIGAAVYLECSSKTQQNVKAVFE",
    "response#2": "IKCVTVGDGAVGKTCMLISYTSNTFPTDYVPTVFDNFSANVVVDGSTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLLSRASYENISKKWIPELRHYAPTVPIVLVGTKLDLREDRQYLIDHPGATPITTAQGEELKKAIGAAVYLECSSKTQQNVKAVFELLAKK",
    "response#3": "IKCVTVGDGAVGKTCMLISYTSNTFPTDYVPTVFDNFSANVVVDGSTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLLSRASYENISKKWIPELRHYAPTVPIVLVGTKLDLREDRQYLIDHPGATPITTAQGEELKKAIGAAVYLECSSKTQQNVKAVFE"
  },
  {
    "reference": "MALSGKHILLIISGGIAAYKSLDLIRRLKERGAKVTPVMTKGAQEFVTPLAVGALSATHVFTELFSRQDEQDVGHIRLARDCDLVLVAPATADLMAKMAHGLADDLASTVLLATDRKVLVAPAMNPKMWEAQATKRNIATLKQDGISFVGPMAGEMAEKGEAGVGRMAEPLQIVATVEDLLDDAAKPLAGKTAIVTSGPTHEPIDPVRYIANRSSGRQGHAIAAALAKLGADVTLVSGPVTIPDPAGVRTIHVERAEEMRDAVVSKLPADIAVFVAAVADWRVAASADQKIKKQPGDAPPALQLTENPDILKTVGHHGKRPKLVVGFAAETQNIEENGRAKLERKGADFIIANDVSAETGIMGGANNQVRVISKSGIDAWPEMTKAEVADRLATLIAETLA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should take into account the physiological conditions of the CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis pathway.2. The designed protein must be able to perform phosphopantothenate--cysteine ligase activity under specific conditions.3. The designed protein should have a binding site that is specific to the target CTP ligand. The designed protein sequence is ",
    "response#1": "MNIVVGITGGIAAYKAVSVVRSLVLAGHDVHVVPTESALRFVGRPTLEAISRNPVHVDLFEGVAEVRHVAIGQAADLIVIAPATANSIAKLATGLADDLLGNTVLASEAPVVVAPAMHTEMWRHPATQANIATLRDRGVTIVGPAVGQLTGADSGPGRLEEPDVIVAAVEASLAAEASHAPARLSGRCIVVTAGGTEEPIDAVRILANRSSGRQGIALAEAARDRGADVTLIAARVSVPLPEGVTIVRAMSVDALDAALRNALLAAPNGSAVTPADALIATAAVSDFRVAGGPLTEKLRRDGEITLRLTPTPDLLAGVAQALGPRATRQTLLVGFSAEVSATPRAREKLEKKDLDLIVANDVTAPGIGFESPENAAEVLVAGGEARPLARATKSDLAHAILDAAERLPA",
    "response#2": "MNIVVGITGGIAAYKAVSVVRSLVLAGHDVHVVPTESALRFVGRPTLEAISRNPVHVDLFEGVAEVRHVAIGQAADLIVIAPATANSIAKLATGLADDLLGNTVLASEAPVVVAPAMHTEMWRHPATQANIATLRDRGVTIVGPAVGQLTGADSGPGRLEEPDVIVAALEEAVRRPRHDLAGRRVVVTAGGTREPLDPVRFLGNRSSGKQGVAIAEAAAARGAEVTLIGANLEVASPGGCRVVPVSTALELQQAVTSAAGDADVVVMAAAVADFRPADVSDVKIKKVDGQEHMSIELVRNPDILAGLGAAPHDGTVLVGFAAETESDPSRLLELGRAKRRAKGADLLVVNRVGWNHGFAADENAVSVEVLEPDGSRTPLARGTKQALAHTLWDVVVRRR",
    "response#3": "MNIVVGITGGIAAYKAVSVVRSLVLAGHDVHVVPTESALRFVGRPTLEAISRNPVHVDLFEGVAEVRHVAIGQAADLIVIAPATANSIAKLATGLADDLLGNTVLASEAPVVVAPAMHTEMWRHPATQANIATLRDRGVTIVGPAVGQLTGADSGPGRLEEPDVIVAALEARLGG"
  },
  {
    "reference": "MSVVVLAAGAGKRMRSPLPKVLQPLAGRTLLDHVLHCAEALRPAATHVVYGHGGEAVRRHFAERAELRWVEQAQRLGTGHAVAQAMPGIPEDHLVLVLCGDVPLLRAATLEALLQGTGEGVGVLSVELPDPTGYGRIVRDSSGAVAAIVEERDADAATRAVREVNTGVIAAPAGRLRGWLSRLGNDNAQGEYYLTDIIAMAVAEGVSVAGVRAADADEVAGINTRAQLAAAESALRRRIAAALLDQGAILADPARLDVRGEVTVGQDVFLDANVTLEGRVELGDGVHIGPNVVLRDVCLGAGTVVHAFSLLEGMRCGTDCQLGPYARVRPGSELAERVKIGNFVEVKNSRLAEGAKANHLSYVGDTDVGRDANIGAGTITCNYDGANKHRTVIGDGAFIGSGVELVAPVEIGPGATIGAGSTISKNAPPHQLTLARARQTTLRHWQRPTKQRSGEDH",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The Proton acceptor of the protein must be engineered to enable effective catalysis of acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.2. The designed protein should interact with enzymes and substrates within the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway to enhance or regulate its activity.3. A protein that contributes to regulation of cell shape, cell morphogenesis, peptidoglycan biosynthetic process, cell wall organization by functioning in cytoplasm with UDP-N-acetylglucosamine diphosphorylase activity, magnesium ion binding.4. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.5. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).6. The protein should be able to bind Mg(2+), acetyl-CoA, UDP-N-acetyl-alpha-D-glucosamine ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MAAGKGTRMRSRWPKVLHRLAGRALVDHVIESAVALQARKVVVITGHGAADVEAAVRRTTAQPSGPADLSFVRQEPQLGTGHAVQQAVPVLPDDGVALILNGDVPLIRAETLRALLQACAGKQKLALLTVHLPDPTGYGRILRKASGQVVRIVEQKDASPAEQAITEVYSGIMAAPSKLLKAWVSRLSNNNAQGEYYLTDIVEMAVADGVAVESAQPAAAWEVLGVNSKVQLAELERIHQRNVAARLLEAGVTVVDPATTWIDAGVTLARDVVIEPGVQLKGTTSVGEGSTVGPDSTLTNVSIGAGASVVRVHGSDSELGDGVNVGPFTYLRPGTKLGVKAKLGAFVETKAADIGAGTKVPHLTYVGDATIGEHSNIGCSSVFVNYDGVNKHRTVIGSYVRLGADNTFVAPVHIGDGAYSGAGAVIREDVPPGTLAVSAPPKRIIKNWKRPVKKP",
    "response#2": "MAAGKGTRMRSRWPKVLHRLAGRALVDHVIESAVALQARKVVVITGHGAADVEAAVRRTTAQPSGPADLSFVRQEPQLGTGHAVQQAVPVLPDDGVALILNGDVPLIRAETLRALLQACAGKQRLGLLTVELPDPTGYGRIVRDAAGGVARIVEHKDASPAERALREVNTGIMVMPAAALRHWLARLRNDNAQGEYYLTDIVAQAVADGVAVVAAQPATEWETLGVNSKVQLAELERAWQLANAQRLLTAGVTVVDPATTWIDAGVTLARDVVIEPGVQLKGTTSVGEGSTVGPDSTLTNVSIGAGASVVRVHGSDSELGDGVNVGPFTYLRPGTKLGVKAKLGAFVETKAADIGAGTKVPHLTYVGDATIGEHSNIGCSSVFVNYDGVNKHRTVIGSYVRLGADNTFVAPVHIGDGAYSGAGAVIREDVPPGTLAVSAPPQRIGLRRPARPEA",
    "response#3": "MAAGKGTRMRSRWPKVLHRLAGRALVDHVIESAVALQARKVVVITGHGAADVEAAVRRTTAQPSGPADLSFVRQEPQLGTGHAVQQAVPVLPDDGVALILNGDVPLIRAETLRALLQACAGKQRLALLTVNLPDPTGYGRILRDGHGQVRAIVEEKDCSPGQRAIREVYTGMMAAPTALLTEWLARLAPANAQREYYLTDIVALAVADGVPVAGVQPLDPVEVEGANDPWHLAQLERAHQLRQAQALMLEGVTLVDPRRIDVRGKLACGRDVLIDINAVFEGEVELGDGVQIGANAVIRDTSIGAGTVIHPHCVLEQSRIGANCQIGPYARTRPGVEMADDAKLGNFVEIKKSRIGKGSKINHLSYIGDAIIGANVNVGAGTITCNYDGANKHRTTIGDGAFIGSGVELVAPVEIGAGATVGAGSTITKAVPADELVLERSQQVSLAGWRRPKKP"
  },
  {
    "reference": "MVLKVKMGDIGVGKSPESIETLLGSCVAIILYDKGKKIGGLAHVMLPKSRMQKEKNPGKYVDTAIPELITKIVKMGARKDRLTVKLAGGAAMFGNNSNNIDVGKKNIEASKIEIKKIGLRVSSEDLGGDTGRTITLSLKDGSVMVRKGSELKTI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must be able to protein-glutamine glutaminase activity. The designed protein sequence is ",
    "response#1": "PEPPDIIAIGSSTGGPQALFTVLGTMKAGTVRQPIVITQHMPATFTTILAEHIGRVSGWEAREAQDGEAIRGGRVYIAPGDFHMVVETKGTDKVLRLNKNPPENFCRPAVDPMLRSIAAAYGKRVLACILTGMGADGMKGGQAVVASGGTVIAQDEASSVVWGMPGAAATAGICSAVLPLPEIAPWIMKLAARA",
    "response#2": "PEPPDIIAIGSSTGGPQALFTVLGTMKAGTVRQPIVITQHMPATFTTILAEHIGRVSGWEAREAQDGEAIRGGRVYIAPGDFHMVVETKGTDKVLRLNKNPPENFCRPAVDPMLRSIAAAYGKRVLACILTGMGADGMKGGQAVVASGGTVIAQDEASSVVWGMPGAAATAGICSAVLPLPEIAPWIMKLAARS",
    "response#3": "IAIGSSTGGPQALFTVLGTMKAGTVRQPIVITQHMPATFTTILAEHIGRVSGWEAREAQDGEAIRGGRVYIAPGDFHMVVETKGTDKVLRLNKNPPENFCRPAVDPMLRSIAAAYGKRVLACILTGMGADGMKGGQAVVASGGTVIAQDEASSVVWGMPGAAATAGICSAVLPLPEIAPA"
  },
  {
    "reference": "MDKRRAHVWISGRVHGVAFRAYTQEAAYHLGIGGWVRNLPDGRVEAVFEGDADKVARMVEWCRKGSPMSRVTEVEVREEDFRGEFVRFEITFGRWGA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein contains novel Acylphosphatase-like domains that confer a unique function or activity.2. The designed protein must be able to perform acylphosphatase activity under specific conditions. The designed protein sequence is ",
    "response#1": "EIYVSGRVQGVYFRGFTQKTATSLGLMGYAQNLPDGRVKVVAQGKRSCISELLDHLHIGPELSNVECIEVGWMGLSDTFTDFFS",
    "response#2": "WHMTAHGRVQGVGYRAGCAQAAIALGVRGWVRNRADGTVEVMASGTIQQLEALRNWMQAGPPAAHVARVDVEPGQGKFEDFL",
    "response#3": "RLTAWVHGWVQGVGFRWWTRCRALELGLTGYAANHADGRVLVVAQGPRAACQKLLQLLQGDTTPGRVAKVVADWSQSTEQITGFSEL"
  },
  {
    "reference": "MPKRLDINTILVIGSGPIVIGQAAEFDYSGTQACQSLKEEGYKVILVNSNPATIMTDTATADKVYIEPLTLEFVSRIIRKERPDAILPTLGGQTGLNMAVELAKSGVLDECGVEILGTKLSAIEQAEDRDLFRTLMQDLNEPTPPSEIIHNLDEAYGFVNEIGYPVIVRPAFTLGGTGGGICHNEEELIEIVTSGLKHSPVTQCLLEKSIAGCKEIEYEVMRDSNDNAIVVCNMENIDPVGVHTGDSIVVAPSQTLSDREYQMLRNTSLRIIRALGIEGGCNVQLALDPYSFQYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEIVNPVTQKTYACFEPALDYVVSKIPRWPFDKFESANRTLGTQMKATGEVMSIGRNLEESLLKAVRSLELGIYHLELDHLKELDKETMKKRIIKADDERLFIVAEAIRQGVTKEEINEWCEMDFFFLQKVENIVNMEREVKANVGNMEVLQTAKEMGFSDHYIAAAWNKTEREIYDMRKENNMMPVFKMVDTCAAEFESATPYYYSTYADENESIVTDRKSVVVLGSGPIRIGQGVEFDYATVHSVWAIKEAGYEAIIINNNPETVSTDFSISDKLYFEPLTIEDVMHIIDLEKPEGVIVQFGGQTAINLAAKLEEHGVKILGTSLEDLDRAEDRDKFEAALTKLGIPQPVGKTATTVEQAVAIAEEIGYPVLVRPSYVLGGRAMEIVYRQEELLHYMKNAVKVHADHPVLIDRYMVGKEIEVDAISDGENVFIPG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein have ATP-grasp domains with specific sequence motifs that are crucial for its activity.2. We require a protein that can specifically bind to the metabolites produced in the (S)-dihydroorotate from bicarbonate: step 1/3 pathway.3. The designed protein should have carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity to facilitate nitrogen compound metabolic process, 'de novo' UMP biosynthetic process.4. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.5. We need a protein that can perform 2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP +   carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate with high efficiency. The designed protein sequence is ",
    "response#1": "IMDCGLKLNQLRCLLDRGASVVLLPWTAKLQDEQFDALFLSNGPGNPESCEEIVEQLRLVLKGETIKPIFGICLGHQLLAKAIGCSTYKMKYGNRGHNLPCLHRTSGRCLMTSQNHGYAVDLEHLPPGWSELFVNANDGTNEGIVHATKPYFSVQFHPEHHAGPRDTEFLFDIFLESKLLDKLGISQPDWISATSLGEIKKFANEVGFPVLVRPSYVLSGSSMKIAYSEEELYEYVRRATEISPKYPVVISKYIENAIEAEIDGVSDGTRIIVAVDCGLKYNQIRCLIKRNAKVILVPWNYKFDTEQYDGIFISNGPGDPEVCKVTVDNLRQRLQDKKNVKPIFGICLGHQLLATAAGCKTYKTRYGNRGHNLPCTHNGTGRCFMTSQNHGFAVDAKTLPENWDILFTNENDKTNEGISHKSLPYFSVQFHPEHTAGPTDLECLFDIFLNHL",
    "response#2": "MVGYPEALTDPSYNSQILVLTYPLIGNYGIPQDEEDEYGLSKWFESTKIHVAALIVGENSQDPSHWSSVRSLDQWLKEHGIPALEGIDTRSLTKKIREEGVLLGAERVSEIGEQVAPSAAVYSVKEALEAAEKIGYPVMARAAFALGGLGSGFANNKEELTALAQQSFGYSNQLIIDKSLKGWKEVEYEVVRDAYNNCITVCNMENVDPLGIHTGESIVVAPSQTLSNREYNMLRTTAIKVIRHFGIVGECNIQYALNPYSEEYFIIEVNARLSRSSALASKATGYPLAYVAAKLSGEPILHAEKINAIGEKVAPSAAVSSVEEALAAALQIGYPVMSRSAFSLGGLGSGFANNEEELRVLAHHALSHSEQLIIDKSLKGWKEVEYEVVRDAYDNCITVCNMENVDPLGIHTGESIVVAPSQTLSNREYYMLRNTAIKVIRHFGIVGECNIQYALNPNSEEFFIIEVNARLSRSSALASKATGYPLAYVAAKLAGQPLPHMAIIEVNARVKLVHAQDDRLFYIAEAFRRGYSLADLHELTRINHYFLDIVAGLVEEEGRIKAAGLDKEVIYEAKRDGFADTTLATAFVALEAVDCGLKYNQIRCLIKRNAKVILVPWNYKFDTEQYDGIFISNGPGDPEVCKVTVDNLRQRLQDKKNVKPIFGICLGHQLLATAAGCKTYKTRYGNRGHNLPCTHNGTGRCFMTSQNHGFAVDAKTLPENWDILFTNENDKTNEGISHKSLPYFSVQFHPEHTAGPTDLECLFDIFI",
    "response#3": "IMDCGLKLNQLRCLLDRGASVVLLPWTAKLQDEQFDALFLSNGPGNPESCEEIVEQLRLVLKGETIKPIFGICLGHQLLAKAIGCSTYKMKYGNRGHNLPCLHRTSGRCLMTSQNHGYAVDLEHLPPGWSELFVNANDGTNEGIVHATKPYFSVQFHPEHHAGPRDTEFLFDIFLIRYELKEAGAERIWYIADAFRAGMSVDGVFNLTNIDRWFLVQIEELVKLEAEVKAGGFAGLNQDVLRKMKRKGFSDARLSKLLGVSENEIRRLRDQYNIHPVYKRVDTCAAEFKSDTAYMYSTY"
  },
  {
    "reference": "MCSTLKKCGTYRTEVAECHDHGSTFQGRKKGGSSFRDNFDKRSCHYEHGGYERPPSHCQENDGSVEMRDVHKDQQLRHTPYSIRCERRMKWHSEDEIRITTWRNRKPPERKMSQNTQDGYTRNWFKVTIPYGIKYDKAWLMNSIQSHCSDRFTPVDFHYVRNRACFFVQDASAASALKDVSYKIYDDENQKICIFVNHSTAPYSVKNKLKPGQMEMLKLTMNKRYNVSQQALDLQNLRFDPDLMGRDIDIILNRRNCMAATLKIIERNFPELLSLNLCNNKLYQLDGLSDITEKAPKVKTLNLSKNKLESAWELGKVKGLKLEELWLEGNPLCSTFSDQSAYVSAIRDCFPKLLRLDGRELSAPVIVDIDSSETMKPCKENFTGSETLKHLVLQFLQQYYSIYDSGDRQGLLGAYHDEACFSLAIPFDPKDSAPSSLCKYFEDSRNMKTLKDPYLKGELLRRTKRDIVDSLSALPKTQHDLSSILVDVWCQTERMLCFSVNGVFKEVEGQSQGSVLAFTRTFIATPGSSSSLCIVNDELFVRDASPQETQSAFSIPVSTLSSSSEPSLSQEQQEMVQAFSAQSGMKLEWSQKCLQDNEWNYTRAGQAFTMLQTEGKIPAEAFKQIS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The presence of TAP-C, NTF2, RRM domains is critical for the interaction of the designed protein with its intended targets.2. The protein must exhibit the following characteristics: Involved in the export of mRNA from the nucleus to the cytoplasm..3. The designed protein should be involved in poly(A)+ mRNA export from nucleus, mRNA export from nucleus by localizing to cytoplasm, cytosol, nucleoplasm, nuclear RNA export factor complex, nucleus and exhibiting RNA binding. The designed protein sequence is ",
    "response#1": "EKHYLFLKRLCQVLCALGNLLCALLALDANIQTPINFGMYLESFLAFTTHPSQFLRSSTHMTWGALFRHEVLSRDPALLAVIPKYLRASMTNLVKMGFPSKTDSPSCEYSRFDFDSDEDFNAFFNSSRAQHGEVVRCVCRLDPKTSFQMAAEWLKYQLSASIDTGPVNSCSTAGTGEGGFCSIFSPSYVQWEAMTFFLESVINQMFRTLDKEELPVSDGIELLQLVLNFEIKDPLVLSCVLTNVSALFPFVTYKPAFLPQVFSKLFSFVTFESVGESKAPRTRAVRNVRRHACSSINKMCRDYPDLVLPNFDMLYSHVKQLLSNELLLTQMEKCALMEALVLVSNQFKDYERQKLFLEELMAPVVNIWLSEEMCRALSDIDSFIAYVGADLKSCDPAVEDPCGLNRARMSFCVYSILGVMRRTSWPSDLEEAKAGGFVVGYTPSGNPIFRNPCTV",
    "response#2": "EKHYLFLKRLCQVLCALGNLLCALLALDANIQTPINFGMYLESFLAFTTHPSQFLRSSTHMTWGALFRHEVLSRDPALLAVIPKYLRASMTNLVKMGFPSKTDSPSCEYSRFDFDSDEDFNAFFNSSRAQHGEVVRCVCRLDPKTSFQMAAEWLKYQLSASIDTGPVNSCSTAGTGEGGFCSIFSPSYVQWEAMTFFLESVINQMFRTLDKEELPVSDGIELLQLVLNFEIKDPLVLSCVLTNVSALFPFVTYKPAFLPQVFSKLFSFVTFESVGESKAPRTRAVRNVRRHACSSINKMCRDYPDLVLPNFDMLYSHVKQLLSNELLLTQMEKCALMEALVLVSNQFKDYERQKLFLEELMAPVVNIWLSEEMCRALSDIDSFIAYVGADLKSCDPAVEDPCGLNRARMSFCVYSILGVMRRTSWPSDLEEAKAGGFVVGYTPSGNPIFRNPCTV",
    "response#3": "EKHYLFLKRLCQVLCALGNLLCALLALDANIQTPINFGMYLESFLAFTTHPSQFLRSSTHMTWGALFRHEVLSRDPALLAVIPKYLRASMTNLVKMGFPSKTDSPSCEYSRFDFDSDEDFNAFFNSSRAQHGEVVRCVCRLDPKTSFQMAAEWLKYQLSASIDTGPVNSCSTAGTGEGGFCSIFSPSYVQWEAMTFFLESVINQMFRTLDKEELPVSDGIELLQLVLNFEIKDPLVLSCVLTNVSALFPFVTYKPAFLPQVFSKLFSFVTFESVGESKAPRTRAVRNVRRHACSSINKMCRDYPDLVLPNFDMLYSHVKQLLSNELLLTQMEKCALMEALVLVSNQFKDYERQKLFLEELMAPVVNIWLSEEMCRALSDIDSFIAYVGADLKSCDPAVEDPCGLNRARMSFCVYSILGVMRRTSWPSDLEEAKAGGFVVGYTPSGNPIFRNPCTV"
  },
  {
    "reference": "METTFRSALVKNFLGQSPDWYKLAILVFLVVNPLVFFLVDPFIAGWLLVVEFIFTLAMALKCYPLLPGGLLAIESVLIGMTSPDRVGEEIAHNLEVLLLLIFMVAGIYFMKQLLLFVFTKLLLNIRSKMLLSLAFCFAAALLSAFLDALTVVAVVISVATGFYSIYHNVASNRSDGNDGNIDVGDDSTLVSDDHKQTLEQFRAFLRSLLMHAGVGTALGGVMTMVGEPQNLIIAKSAGWGFVDFFLRMAPVTLPVFACGLLVCLLLERFGVFGYGAKLPERVREVLTEFDRQATAGRSKQEQVRLVVQALIGIWLVVALAFHLAEVGLIGLSVIILATSLCGVTDEHAIGKAFQEALPFTALLTVFFTVVAVIIEQHLFTPIIHFVLQAQPSSQLALFYLFNGLLSSVSDNVFVGTVYINEARAAFENGVISLKQFEMLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLVRLSYGRMVWMALPYTVVLTLVGLLCVQFTLAPATELLTQWHWLTLPSIEAATH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have sodium:proton antiporter activity and be active in plasma membrane.2. The protein should have Helical domains to ensure proper insertion into the membrane.3. The protein should have a fast turnover rate for 3 H(+)(out) + 2 Na(+)(in) = 3 H(+)(in) + 2 Na(+)(out). The designed protein sequence is ",
    "response#1": "LTTLLTYVNYRFIRLPPTIGVMATALVFSLIVQGLSELGYPILEVEMQEIIRRIDFSEVLMTWFLPALLFAGALHVDLSDLRSYKWPIGLLATAGVLIATFVIGGLAYYTFPLFGWQVDFIYCLLFGALISPTDPIAVLGILKSAGAPKPLATTIVGESLFNDGTAVVVFAIILGILQLGEAPTVSATAILFVQEAIGGVVFGAVLGYGVFVMMRGIDQYQVEVMLTLALVIGGAALAARLHVSAPIAMVVAGLIIGNHGRHYAMSDETRRYVDKFWELIDEILNALLFALIGLELLLLPFSWLHVAAAFALGGAVLVSRLLTVGPAILVLRRFRGANRQVPAGTIRILVWGGLRGGVSVALALSLPLGPERDLILSLTYIVVLVSILLQGLSIGPLVYKWLL",
    "response#2": "LTTLLTYVNYRFIRLPPTIGVMATALVFSLIVQGLSELGYPILEVEMQEIIRRIDFSEVLMTWFLPALLFAGALHVDLSDLRSYKWPIGLLATAGVLIATFVIGGLAYYTFPLFGWQVDFIYCLLFGALISPTDPIAVLGILKSAGAPKPLATTIVGESLFNDGTAVVVFAIILGILQLGEAPTVSATAILFVQEAIGGVVFGAVLGYGVFVMMRGIDQYQVEVMLTLALVIGGAALAARLHVSAPIAMVVAGLIIGNHGRHYAMSDETRRYVDKFWELIDEILNALLFALIGLELLLLPFSWLHVAAAFALGGAVLVSRLLTVGPAILVLRRFRGANRQVPAGTIRILVWGGLRGGVSVALALSLPLGPERDLILSLTYIVVLVSILLQGLSIGPLVALLVR",
    "response#3": "LTTLLTYVNYRFIRLPPTIGVMATALVFSLIVQGLSELGYPILEVEMQEIIRRIDFSEVLMTWFLPALLFAGALHVDLSDLRSYKWPIGLLATAGVLIATFVIGGLAYYTFPLFGWQVDFIYCLLFGALISPTDPIAVLGILKSAGAPKPLATTIVGESLFNDGTAVVVFAIILGILQLGEAPTVSATAILFVQEAIGGVVFGAVLGYGVFVMMRGIDQYQVEVMLTLALVIGGAALAARLHVSAPIAMVVAGLIIGNHGRHYAMSDETRRYVDKFWELIDEILNALLFALIGLELLLLPFSWLHVAAAFALGGAVLVSRLLTVGPAILVLRRFRGANRQVPAGTIRILVWGGLRGGVSVALALSLPLGPERDLILSLTYIVVLVSILLQGLSIGPLVAAMV"
  },
  {
    "reference": "MSPLKKTYVLKLYVAGNTPNSVRALKTLNNILEQEFQGVYALKVIDVLKNPQLAEEDKILATPTLAKILPPPVRKIIGDLSDREKVLIGLDLLYEEFAEAEAEAE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: KaiB(fs) binds phospho-KaiC, KaiA and CikA; KaiA and CikA compete for binding to KaiB(fs), and KaiB(fs) and SasA compete for binding to KaiC, thus the clock oscillator and output signal pathway are tightly coupled.; A metamorphic protein which reversibly switches between an inactive tetrameric fold and a rare, thioredoxin-like monomeric fold (KaiB(fs)).2. The designed protein must be able to regulate circadian rhythm, negative regulation of phosphorylation. The designed protein sequence is ",
    "response#1": "MVAHELRTPLSAAKLALQSQALGQIDLIKLQEVVKRRLEEIESLSKDLLEVGTTRWEALFNPQEANLANIAAEVILELEKFWLSRGIGINTDIPADLPNVFADQSRMKQVLLNLIENALKFSNDGDTVEITMLHRTNQWVQISVSDKGPGIPEEEQQRIFLDRVRLPQTSNETSGFGIGLSVCRRIVEVHGGKIWVVSQPGEGSCFYFTVPVWR",
    "response#2": "MVAHELRTPLTAATLAVQSQELGQIDIHRFRDVLKRRLEEIALLSKDLLEVGSTRWEALFNPQRLDLTSVAAEAILELEKLWLGRDVTIHTDIPADLPKVFADQRRMRQVLLNLLENALKYTPNGGLISLTMLHRTSQWVQVSISDSGPGIPEEEQQRIFLDRVRLPQTSAGASGFGVGLSVCRRIVEVHGGRIWVISEPEKGACFTFNVPIWR",
    "response#3": "MVAHELRTPLSAAKLALQSQALGQIDLIKLQEVVKRRLEEIESLSKDLLEVGTTRWEALFNPQEANLANIAAEVILELEKFWLSRGIGINTDIPADLPNVFADQSRMKQVLLNLIENALKFSNDGDTVEITMLHRTNQWVQISVSDKGPGIPEEEQQRIFLDRVRLPQTSNETSGFGIGLSVCRRIVEVHGGKIWVVSQPGEGSCFYFTVPVWN"
  },
  {
    "reference": "MNQPIAAIGISDSTRPKTNVRLTISAEHLMDTDVFSKSDPICLVYEKTSGKKATTTEPVQVPTWQDSQWTERGRTEVVMNNLNPQFTKTFLLPYFFEETQLLRFEIYDADSPNVGQDLSPHDFLGRFECVLAQIVSYSTLKAHLGKAEGIGAQWRNKDKNTRTGSITIRAEEDEKCEKIQFDVCGEGLDKKDFFGKSDPYLNFKRKFDDGSAHLVHRTEVKQKTLDPRWATVQINTQTLCGKDGERPIIIECYDHDKWKKGEEPRGEAKFSRDDHIGTAHTTLNELLRGGAGDPVELLLTNEKKKAKKGDKYKCSGTLKIWNSKIIVEPTFLDFISGGTQLEFAVAVDFTASNGAPKNSSSLHYMSADRPNQYELALRSVLSICQHYNSSKTFEAFGFGAKLPNSVSVSAIFSLDLLRGTPEVVGISGVMSAYRHALQNVQLYGPTNFAPIIDTVAQKAQNMIHDSARYQILLIITDGIISDMHATIRSIINASGLPLSIIIIGVGNEDFEKMHELDSDDSLLQQDSRIAQRDIVQFVTIREFLNNGRGVYLDPDVVQENLAREVLFEVPGQLTGYMKQRGFQPRPVENPWTRNSPPPDYDPVLDGIGRRAQAPSPGFQMPVASAPPMY",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Involved in nicotinic acetylcholine receptor (nAChR)-mediated sensitivity to nicotine and levamisole; Affects directional sperm motility.; Exhibits calcium-dependent phospholipid binding properties; May function in membrane trafficking.2. The designed protein should be optimized for calcium-dependent phospholipid binding activity in order to improve cellular response to calcium ion.3. The designed protein must have at least one C2, VWFA domain. The designed protein sequence is ",
    "response#1": "QRQKLNWLNLELEKIWPYVNEAASELIKSSVEPVLEQYTPAMLASLKFSKFTLGTVAPQFTGVSILESESGPNGITMELEMQWDGNPKIVLDVKTLLGVSLPIEVKNIGFTGVFRLIFKPLVDEFPCFGALSYSLREKKGLDFTLKVIGGELTSIPGISDAIEETIRDAIEDSITWPVRKIIPIL",
    "response#2": "NFMVAIDFTASNGNPRLPDSLHYIDPSGRLNAYQRAIMDVGEVLQFYDSDKRFPAWGFGARPIDAPVSHCFNLNGSSSYSEVDGIQGIMTSYTSALFNVSLAGPTLFGPVINAAAMIASASLAQGSRKYYVLLIITDGVITDLQETKDALVSASDLPLSILIVGVGGADFKEMEILDADKGERLESSSGRLASRDIVQFVALRDVQYGEISVVQALLAES",
    "response#3": "QRQKLNWLNLELEKIWPYVNEAASELIKSSVEPVLEQYTPAMLASLKFSKFTLGTVAPQFTGVSILESESGPNGITMELEMQWDGNPKIVLDVKTLLGVSLPIEVKNIGFTGVFRLIFKPLVDEFPCFGALSYSLREKKGLDFTLKVIGGELTSIPGISDAIEETIRDAIEDSITWPVRKIIPIT"
  },
  {
    "reference": "MPTMTKQLRDIIADIDLTLIQGSLDLPITNITDDSRQVTPGSLFIVRSWVHDGASIIGNPSGYARSFIQDAVLLGAIAIIDVHDQDIPTYDSHLTEPIGRPAIPSSITLLTTPPNTKIDQAFVGQLADNFFDHPASKLKLLGITGTNGKTTTAFITQHLLQSFNHKTGLIGTVHIDTGSGIQPTELTTPGSIELHRILAQMVDNNCTHAIMEVSSHALHQRRTHNLQFDAAIFTNLSGDHLDFHPDMNSYAAAKAILFESLSDNAWAIINADDTFARRMAQSCTAKQLLTTTNPDFQTAPHTHHQVCHVNITSLSIDGSSARYTGPWGSTSARLPLIGRHNALNAIQALAALHVLNLPGKTKPRQIRRALESTPGIPGRLEQVFINPSQYDLAALEFNPAHLALDPFTAQPEDPTELPTELPYPTVLVDYSHTDDSLENALLAVRPLALGRVITVFGCGGDRDKTKRPRMASVASEYSDIVVITSDNPRTEDPIAIIEDVIPGIPIPQLSPKAPIEDRIQAFTSETAIGEGGGIIEPDRAKAIHKAILLANPEDVVLIAGKGHETYQILRTQDVTDPDLLKTATPAPKGTVKIHFDDREYAKNALKNWATRFYDSAPE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should take into account the physiological conditions of the peptidoglycan biosynthesis, Cell wall biogenesis pathway.2. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.3. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..4. The protein should be able to bind UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate ligand in a variety of conditions5. The designed protein should contain one or more Mur ligase central, Mur ligase C-terminal domains that are essential for its function.6. The protein should have a low Km value for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.7. The protein's functional design necessitates the inclusion of a Meso-diaminopimelate recognition motif motif. The designed protein sequence is ",
    "response#1": "MAKITGLSVDSRLVKPGHLFAALPGTRVHGGAFIQYALRMGAGAVLTDAEGARIAREELAASDAALVVAEDPRQTLAYAAALWFGAQPETMIGVTGTNGKTTTTHIIEHILNDQQSKTGVIGTIKNEIGDEVIPTERTTPESTDLQELFAKMREAGVTDVAMEVSSHALDLHRVDGCRIDVAVFTNLTQDHLDYHKTMEEYFAAKAKLFTDLGKKSGRFQAVEEGQDFAVLVDYAHTPDSLRRVLEAARAISRGRLIVVFGCGGDRDRGKRPMMGEIAVRLADLAVITSDNPRSEDPLSIIREIEEGARRSGGGGSWRVVPDRREAIGLAVGEASTGDVVVIAGKSDEGAEIVAVETPFGKVKLSSPLIGAYNLENILAAAGFGLVAGLEPGKIAEGIERLDAVPGRMEAFSAPGKATVVVDYAHTPDALRQALKATRAHCSGKLWCVFGCGGDRDQGKRPLMALEADKAADCLVLTADNPRTESVETIIAQMKQGLLGHAKVWQQSDRAEAVKLALTLAEAKDIILVAGKGHETYQIVGDRIKRLITDSRACQAGDLFIGMTGTQVDGGKFAPMAIAQGAIAVIISQQAFAELATELQTRAIAVDDVVLACSKLATAFG",
    "response#2": "MNSIKRLITDSRACQAGDLFIGMTGTQVDGGKFAPMAIAQGAIAVIISQQAFAELATELQTRAIAVDDVVLACSKLATAFYGSPSRSLKLVGITGTSGKTTSSYLIEAGLAAAGRSTGLVGTIETRVVGRRVPSALTTPEAPQLHALFAAMHERGIDTVVMEVSSHALALGRVDGIRFSIGAFTNLSQDHLDFHKDFDDYFATKGRLFAADSTVRADRAVICVDDEWGVRMSDIARRAHPDQADAVATIGTCPDVQRERDEPPDWIAGPSHTTKSGRQVFALTGPDGATREVSLRLPGQYNVANAALAAAVTGATLRAQQAQPGDLFAGLPGARAHGAQFIAQAVEGGAIALFTDAAGAELPEVRAAGLPVLVHPDPRAVLGELAARILGVEVDEIVAGLSQVVVPGRLERVDEGQEFVTLVDYSHKPGAVEAVLEALRPVTEGRLAIVIGCGGDRDRGKRPLMGEAAARLADMAYFTNDNPRSEDPLTILAAMVEGGLKVPQRERAHIVVEPDRAAAIEMAVGRARRGDVVVVAGKGHETYQIIGTERRPFDDREVVRELLQAR",
    "response#3": "MVIEIEGLTTNSHATNVGELFVGMPGTRVDGGEFWQSALAAGAVAAIISTAAAAKVPPTADVCVITTSDMTKACAKISAAFYGFPSRKMTVIGVTGTNGKTTTTHLIEFLLDKASLKTALMGTLYTRWQGFSQTAVHTTPFAVELQQQLAAAVDAGNEYGVMEISSHALAQGRVLGCKFEVGVFSNLTQDHLDYHKDMEDYFNAKALLFSPNYLQGRAIINIDDSYGQRLIQALGDEKVWSYSVNNTQANLWMSDLNYQQNGISGTLHTPKGNVKFHSPLVGQYNLENLLAAVGCGLVLGMETAAILAALEQFKGVPGRMEAFSAPGKATVVVDYAHTPDALRQALKATRAHCSGKLWCVFGCGGDRDQGKRPLMALEADKAADCLVLTADNPRTESVETIIAQMKQGLLGHAKVWQQSDRAEAVKLALTLAEAKDIILVAGKGHENYQIIGSKTLPFSDVNDARKALSDRW"
  },
  {
    "reference": "MKEVFSVGGKELTSRLLIGSGKYSTNKLIPAILDASGSQVITMAMRRVDTEFTEENILNYIPSDCVLMPNTSGARNAQEAIRIARLARAAGCGDWVKIEVISDNRYLLPDNYETIRATEVLTAEGFQVFPYMSPDLMVAKELERVGAAAVMPLGAPIGSNRGLQTRELVRILIEEISLPVIVDAGIGRPSEAAEAMEMGAAAVLVNTAVATAKDPVAMARAFGLAVEAGRTAYLAGPGATQQVARASSPLTGFLREE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for 1-deoxy-D-xylulose 5-phosphate binding. The designed protein sequence is ",
    "response#1": "MSVPDMKIPIMYTLSWPDRSAILCKKLNLASCNQLTFIKPDVYKFPGIKLGFEVLKTSNVHANSIILNAANEVAVDAFLNKKIRFLDIVSVVCETLNLVNYGRINSLSSILDCDLVSRR",
    "response#2": "VSIGTSGVALSIENSTDYENDGNVHYFNHCVPNQKYIMGVTLSAGYSLEWLKQLISADENFTTFLKDINQSEVGANGLMYTPYLLGERTPHNDASVRGSFIGLDANTTQLDMKRAVIEGITYSINESIHIMKNNAININEIVSIGGGAKNNQWLQIQADIFNTTITTRTEEQGPAYGAAMIAAML",
    "response#3": "MSVPDMKIPIMYTLSWPDRSAILCKKLNLASCNQLTFIKPDVYKFPGIKLGFEVLKTSNVHANSIILNAANEVAVDAFLNKKIRFLDIVSVVCETLNLVNYGRINSLSSILDCDLVSRR"
  },
  {
    "reference": "MNSHLSERASQSGGLSHAQPRVLFGVTGGIAAYKAVTAVRRLRQWGANVVVVPTHAALDMVGKTTWEAISGNPVHVDIPEEAHAVVHVNTGAQADLFVVAPGTAHTIAKLAHGLADNLLTASALVATCPRLIAPAMHTQMWEHPATQENIATLVRHGWSLIGPANGQLTGSDSGIGRMEEPENIAHEAIAMLEKLGFNAAGTPENTGLTWVISAGGTHEAIDPVRYIGNHSSGLMGVHIANAARACGHNVTLVAANITSTVLEKCAPGVMIVPVTSARDVHETMDHYAHAANVIVMAAAISDYRVVESATKIKRGGELTLHLEPNPDILHDLATNRRHPGQCVVGFAAETGDNTTSYLDYGKQKARRKGADLLVINKVGGGDGFGDVATTVTIVTSNATEEGSFSGTKIRVAQKIVETIERLSHNGTQKEERP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..2. The designed protein should have metal ion binding, phosphopantothenate--cysteine ligase activity to facilitate pantothenate catabolic process, coenzyme A biosynthetic process.3. The protein contains novel DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains that confer a unique function or activity.4. We require a protein that can specifically bind to the metabolites produced in the CoA from (R)- pantothenate: step 3/5 pathway. The designed protein sequence is ",
    "response#1": "MRLVVGVSGGIAAYKAVGVIRAFVQDGHEVQVIATPSAYEFVGKATLEAISRQPVFDSLYDDVSQVRHVELGQNADAVVIAPATAHTLASLTAGLAPDLLGNAVLARRGPLVVAPAMHTEMWTNAATVHNISVLRTRGVIIVDPATGPLTGQDSGVGRMAEVHSIVALLERFAAGGALAGTRVVVTAGRTEEPLDPVRVLTNRSSGRLGVALARVFRLAGADVRLVCGAVSVPLPASIPATPAPTADAMLVAVTDAAADCDILVMCAAVGDYRPASVSRNKTHATRLTLVLEKTPDILKAVTASKRPPVVIGFSHDPSVAAAREKLRRKRLDLVVANPFETAQPATLAALEAGATLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHLVSADRAAEEWPRLPKREVAARLLDLVLDNVRPA",
    "response#2": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALDVLRTPPPPLDLAGTRVVVTAGRTEEPLDPVRVLTNRSSGRLGVALARVFRLAGADVRLVCGAVSVPLPASIPATPAPTADAMLVAVTDAAADCDILVMCAAVGDYRPASVSRNKTHATRLTLVLEKTPDILKAVTASKRPPVVIGFSHDPSVAAAREKLRRKRLDLVVANPFETALVDEHGDVVARASGSKRAVADAVWDLVAPRLG",
    "response#3": "MRLVVGVSGGIAAYKAVGVIRAFVQDGHEVQVIATPSAYEFVGKATLEAISRQPVFDSLYDDVSQVRHVELGQNADAVVIAPATAHTLASLTAGLAPDLLGNAVLARRGPLVVAPAMHTEMWTNAATVHNISVLRTRGVIIVDPATGPLTGQDSGVGRMAEVHSIVAAAVEALRPATGALAGTRVVVTAGRTEEPLDPVRVLTNRSSGRLGVALARVFRLAGADVRLVCGAVSVPLPASIPATPAPTADAMLVAVTDAAADCDILVMCAAVGDYRPASVSRNKTHATRLTLVLEKTPDILKAVTASKRPPVVIGFSHDPSVAAAREKLRRKRLDLVVANPFETAVGILAADGSARRLAGTRVVVTAGRTEEPLDPVRVLTNRSSGRLGVALARVFRLAGADVRLVCGAVSVPLPASIPATPAPTADAMLVAVTDAAADCDILVMCAAVGDYRPASVSRNKTHATRLTLVLEKTPDILKAVTASKRPPVVIGFSHDPSVAAAREKLRRKRLDLVVANPFETAVGVLAADGSARQLAGTRVVVTAGRTEEPLDPVRVLTNRSSGRLGVALARVFRLAGADVRLVCGAVSVPLPASIPATPAPTADAMLVAVTDAAADCDILVMCAAVGDYRPASVSRNKTHATRLTLVLEKTPDILKAVTASKRPPVVIGFSHDPSVAAAREKLRRKRLDLVVANPFETAVGVLAADGSARQLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVH"
  },
  {
    "reference": "MTASSFQRPLYLADQVRELDRRAIDAEGDGFALMRRAGESAYEVLCARWPGIRRVCVLCGGGNNGGDGYIVAALAAADGVAVDLVALKPVAELEGDARRAAELAAEAGVVPVAWNDELTLDGELMVDAMLGTGAHGAPRPPYAAAISEVNASGCPVMALDAPSGVDVDTGHIETEAVAATLTVTFVGDKLGLHTGAALDVVGDVIYAPLGVDPRSHGGIAPVAWLQTRQDLARALPPRRPGSHKGIYGHVLVVAGAPGMGGAALMTSEMVARVGAGKVSLATDAAHVSASLTRFPEIMARGVRGAPDLEAMIESADTVAIGPGIGNGAWGQAALRSVLDAGKPCVVDADALNLLAREESGIHRDDWVLTPHPGEAGRLLGISTAEVERDRRDAVIALQRRWGGTVVLKGAGTLVYDGDRLTLCPFGNPGMASGGMGDALTGIIAGLSGQLASLGEAARIGVLLHALAADAAALDAGERGLLATDLASYARTLANPQPVSSHADHA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.2. The K(+), (6S)-NADPHX, AMP binding site should be located in a region of the protein that is accessible to the ligand.3. Mg(2+) is required for the proper folding and stability of the protein.4. A protein with metal ion binding, NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding, NADPHX epimerase activity functions is required to enable nicotinamide nucleotide metabolic process.5. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.6. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. The designed protein sequence is ",
    "response#1": "MMITDINCECLGLSRLCLMESAGKSLAEEVGKIAVYTFSKPVKIAIFTGSGGNGGDGFVAARYLLNRGYDVDIYMLTDKDNIHSDNAKTNFEIITNMEPRLSHLTVHYLDTIEDVNNCDIAQSESFSEFIIVDAILGTGIKGELRPKVKRAVEVINKSNGLTVSVDVPSGMDPLTGEVSDVAVEPHYTISFHRVKTGIHLAGEDKVGGIVTCDIGIPIEAEGIIGGNTGMNGALQLAGASALYSGCGKVSMIPLDQSFKADMSMPELMTKSLKNVPKNINIFSALAVGVGFDTTDDSQQILESIIDNLTQLAIFDADALNIIVTNQQIREKFINLENKIITPHPAEAARLLDCTTKEIQEDRFTAIRTLAKKYNATVVLKGAGSLICKDNKIYINTTGNQGMAVAGQGDVLSGIIGAFLAQGLNTLSASRLAVYVHGLAGDNVAKKLGIIGGNTGMNGALQLAGASALYSGCGKVSMIPLDQSFKADMSMPELMTKSLKNVPKNINIFSALAVGVGFDTTDDSQQILESIIDNLTQLAIFDADALNIIVTNQQIREKFINLENKIITPHPAEAARLLDCTTKEIQEDRFTAIRTLAKKYNATVVLKGAGSLICKDNKIYINTTGNQGMAVAGQGDVLSGIIGAFLAQGLNTLSASRLAVYVHGLAGDNVAKKLL",
    "response#2": "MMITDINCECLGLSRLCLMESAGKSLAEEVGKIAVYTFSKPVKIAIFTGSGGNGGDGFVAARYLLNRGYDVDIYMLTDKDNIHSDNAKTNFEIITNMEPRLSHLTVHYLDTIEDVNNCDIAQSESFSEFIIVDAILGTGIKGELRPKVKRAVEVINKSNGLTVSVDVPSGMDPLTGEVSDVAVEPHYTISFHRVKTGIHLAGEDKVGGIVTCDIGIPIEAEGIIGGNTGMNGALQLAGASALYSGCGKVSMIPLDQSFKADMSMPELMTKSLKNVPKNINIFSALAVGVGFDTTDDSQQILESIIDNLTQLAIFDADALNIIVTNQQIREKFINLENKIITPHPAEAARLLDCTTKEIQEDRFTAIRTLAKKYNATVVLKGAGSLICKDNKIYINTTGNQGMAVAGQGDVLSGIIGAFLAQGLNTLSASRLAVYVHGLAGDNVAKKLN",
    "response#3": "MMITDINCECLGLSRLCLMESAGKSLAEEVGKIAVYTFSKPVKIAIFTGSGGNGGDGFVAARYLLNRGYDVDIYMLTDKDNIHSDNAKTNFEIITNMEPRLSHLTVHYLDTIEDVNNCDIAQSESFSEFIIVDAILGTGIKGELRPKVKRAVEVINKSNGLTVSVDVPSGMDPLTGEVSDVAVEPHYTISFHRVKTGIHLAGEDKVGGIVTCDIGIPIEAEGIIGGNTGMNGALQLAGASALYSGCGKVSMIPLDQSFKADMSMPELMTKSLKNVPKNINIFSALAVGVGFDTTDDSQQILESIIDNLTQLAIFDADALNIIVTNQQIREKFINLENKIITPHPAEAARLLDCTTKEIQEDRFTAIRTLAKKYNATVVLKGAGSLICKDNKIYINTTGNQGMAVAGQGDVLSGIIGAFLAQGLNTLSASRLAVYVHGLAGDNVAKKLLEA"
  },
  {
    "reference": "GVPCLCDSDGPRPRGNTLSGILWFYPSGCPSGWHNCKAHGPNIGWCCKK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a specific function within the extracellular region, nematocyst.2. For general function, the protein need meet that Selectively modifies sodium channel inactivation from the open state with little effect on channel activation or on inactivation from closed states ; Does not display phospholipid-binding activities, suggesting that the domain IV S3-S4 linker is located at the extracellular surface and not buried in the phospholipid bilayer.; When tested electrophysiologically, this toxin exhibits a high affinity for multiple sodium channels with a 50-fold preference for rat cardiac (Nav1.5/SCN5A) over neuronal channels (0.1 nM versus 5 nM); Binds specifically to voltage-gated sodium channels (Nav) (site 3), thereby delaying their inactivation3. The engineered protein is expected to adopt a turn-strand-strand-based conformation. The designed protein sequence is ",
    "response#1": "RDGYIAQPENCVYHCFPGSSGCDTLCKEKGGTSGHCGFKVGHGLACWCNALPDNVGIIVEGEKCCN",
    "response#2": "RDGYIAQPENCVYHCFPGSSGCDTLCKEKGGTSGHCGFKVGHGLACWCNALPDNVGIIVEGEKCK",
    "response#3": "RDGYIAQPENCVYHCFPGSSGCDTLCKEKGGTSGHCGFKVGHGLACWCNALPDNVGIIVEGEKCK"
  },
  {
    "reference": "MANSSRSRAILSVNLDAVMANDPLRELFEACKTGEIAKVKKLITPQTVNARDTAGRKSTPLHFAAGYGRREVVEFLLNSGASIQACDEGGLHPLHNCCSFGHAEVVRLLLKAGASPNTTDNWNYTPLHEAASKGKVDVCLALLQHGANHTIRNSEQKTPLELADEATRPVLTGEYRKDELLEAARSGAEDRLLALLTPLNVNCHASDGRRSTPLHLAAGYNRIGIVEILLANGADVHAKDKGGLVPLHNACSYGHFDVTKLLIQAGANVNANDLWAFTPLHEAASKSRVEVCSLLLSRGADPTLLNCHSKSAIDAAPTRELRERIAFEYKGHCLLDACRKCDVSRAKKLVCAEIVNFVHPYTGDTPLHLAVVSPDGKRKQLMELLTRKGSLLNEKNKAFLTPLHLAAELLHYDAMEVLLKQGAKVNALDSLGQTPLHRCARDEQAVRLLLSYAADTNIVSLEGLTAAQLASDSVLKLLKNPPDSETHLLEAAKAGDLDTVRRIVLNNPISVNCRDLDGRHSTPLHFAAGFNRVPVVQFLLEHGAEVYAADKGGLVPLHNACSYGHYEVTELLVKHGANVNVSDLWKFTPLHEAAAKGKYDICKLLLKHGADPMKKNRDGATPADLVKESDHDVAELLRGPSALLDAAKKGNLARVQRLVTPESINCRDAQGRNSTPLHLAAGYNNFECAEYLLENGADVNAQDKGGLIPLHNASSYGHLDIAALLIKHKTVVNATDKWGFTPLHEAAQKGRTQLCSLLLAHGADAYMK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Stimulates proteasome activity, probably by ADP-ribosylation of PI31; Modulates 26S proteasome assembly..2. The protein should have a low Km value for L-glutamyl-[protein] + NAD(+) = 5-O-(ADP-D-ribosyl)-L-   glutamyl-[protein] + nicotinamide.3. The designed protein must have at least one PARP catalytic domain.4. The designed protein should have nucleotidyltransferase activity within cytoplasm to regulate positive regulation of telomere capping, positive regulation of canonical Wnt signaling pathway.5. The protein should be able to bind Zn(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MNKITVIQGDITNIASEAIINVANSSLLGGGGVDGAIHRAGGPVILAECQAIRSRQGGCKVGEAVITGAGTLPADYVIHTVGPRWSDGRHNEDTQLKSVYLSCFKLVGHHGIKTVSFPNISTGIYGFPKKRAAAIALDVIKHCIAENRTIEKVNLVCFDAENYDLYLKLLDPLYYHYQQLNCGLTPVGNDSEEFSMVANYMENTHAKTHSGYTVEIAQLFRASRAVEADRFQQFSSSKNRMLLWHGSRLTNWAGILSQGLRIAPPEAPVTGYMFGKGVYFADMFSKSANYCYANTGANDGVLLLCEVALGDMNELLYSDYNADNLPPGKLSTKGVGKTAPNPSEAQTLEDGVVVPLGKPVERSCSKGMLLYNEYIVYNVEQIKMRYVIQVKFNYKK",
    "response#2": "MTLGIFANCIFCLKVKYLPRQQKKKLQTDIKENGGKFSFLLNPQCTHVIVDSADVLSRCHLNSIQKNDVQIANPAFIQDSVRQRRLLDVRNYDSSTKAGLQDASGNLVPLEDVHIKGRIIDTVAQVIVFQTYTNKSHVPIEAKYIFPLDDKAAVCGFEAFINGKHIVGEIKEKEEAQQEYLEAVTQGHGAYLMSQDAPDVFTVSVGNLPPKAKVLIKITYICKSCMEKIEKGQVRLSKKVVYPDKPQLGMVDCWYHPNLTSKLEISVQNLIKLIFDIDSMNKTLMEFHIDMDKMPLGKLSAHQIQSAYRVVKEIYNVLECGSNTAKLIDATNRFYTLIPHNFGVQLPTLIETHQQIEDLRQMLDSLAEIEVAYSIIPSDKPLTGMKLLAVGKLSKNKDDLKAAVEELGGKMTGTANKASLCLSTKKEVEKMTKKMEEVRDAGVRVVSEDFLMPLEKSLSNRGKCGKCGQNFERGEIKAHNKGKANHFKCFLQEFDKISGTVEDIPGWADYEENFKIKAVGEYVPPDKPLSNMKILTLGKLSQNKDEVKATIEKLGGKLTGTANKASLCISTKKEVDKMNKKMEEVKEANVRVVSEDFLQETEEFSRVRKEVLQNNRSEQPVDILQIFRVGRVNEATEFLSKLGNVRLLFHGSPVRNILGILSRGLLLPKVAEDRGVQRTDVGNLGSGIYFSDSLSTSIKYAHAGETDGSRLLVVCDVALGKCVNLFKKDFSLTEAPPGYDSVHGVSETTSVPTDFQDDEFVVYKTNQVKMKYIVKF",
    "response#3": "MTLGIFANCIFCLKVKYLPRQQKKKLQTDIKENGGKFSFLLNPQCTHVIVDSADVLSRCHLNSIQKNDVQIANPAFIQDSVRQRRLLDVRNYDPPSLAKYRALRCKIEHVDQNTEEFSRVRKEVLQNNRSEQPVDILQIFRVGRVNEATEFLSKLGNVRLLFHGSPVRNILGILSRGLLLPKVAEDRGVQRTDVGNLGSGIYFSDSLSTSIKYAHAGETDGSRLLVVCDVALGKCVNLFKKDFSLTEAPPGYDSVHGVSETTSVPTDFQDDEFVVYKTNQVKMKYIVKFCTPGDQIKDPLYYHYQQLNCGLTPVGNDSEEFSMVANYMENTHAKTHSGYTVEIAQLFRASRAVEADRFQQFSSSKNRMLLWHGSRLTNWAGILSQGLRIAPPEAPVTGYMFGKGVYFADMFSKSANYCYANTGANDGVLLLCEVALGDMNELLYSDYNADNLPPGKLSTKGVGKTAPNPSEAQTLEDGVVVPLGKPVERSCSKGMLLYNEYIVYNVEQIKMRYVIQVKFNYKC"
  },
  {
    "reference": "MNNFGRNLALWVIIIVLLMLLFTAFQPGGSQHASQQLAYSDFVTDVDHNRVRSVVVQDQNISGTLTDGTSFETYSPQDPTLISRLTSKNVEVVAKPLDTEGSPLLRYVLNYLPILLMLGVGVLFFRQMQSGSGRAMGFGKSRARLLTEKQGRVTFDDVAGIDEAKSELQEIVDFLKDPQKFTRLGGKIPKGALLVGPPGTGKTLLARAIAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQGKKSAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLNQMLVEMDGFESNEGVILIAATNRPDVLDPALLRPGRFDRQVVVPNPDVMGRERILRVHMRKVPLASDVDPRVIARGTPGFSGADLANLVNEAALLAARLGKRTVAMLEFENAKDKVLMGAERRSLVMSDDEKRMTAYHEGGHALCAILTPGCDPVHKATIIPRGRALGLVMSLPEGDRYSKSKSKCMAELVLAMGGRAAEEIVFGAENVCTGASGDIKMATDQARRMITEWGMSEKLGMIAYGDNGQEVFLGHSVTQNKNVSEQTAREIDREIKQLIDHAYHTAHSLLLSRIDDLHRLAKALLEFETLTGEEIGKVLRGEPIERVVVDDPAPENRRASVPTTRPGAGGTGGLDPAPQPS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The designed protein should be expressed specifically in the plasma membrane The designed protein sequence is ",
    "response#1": "QLDGIPVLFIPGNAGSYRQVRSIAAACSELYFKQSDILINKNAKNLDFFAADFNEDFTAFHGGTMLDQAEYLNDAIRYILSLYDQPDVSTTLAKPKSVIIVAHSMGGIVARLMPTLKNHIHGSVHSYLTLSSPHAAAPITFDGDVLQLYKRTNEYWKRELNDKSSFIFNNVSLISITGGIQDTILPADYAMIEDLIPYSNGFTVHTNTIQDVWTPIDHLAIVWCKQLREIISRYLVA",
    "response#2": "QLDGIPVLFIPGNAGSYRQVRSIAAACSELYFKQSDILINKNAKNLDFFAADFNEDFTAFHGGTMLDQAEYLNDAIRYILSLYDQPDVSTTLAKPKSVIIVAHSMGGIVARLMPTLKNHIHGSVHSYLTLSSPHAAAPITFDGDVLQLYKRTNEYWKRELNDKSSFIFNNVSLISITGGIQDTILPADYAMIEDLIPYSNGFTVHTNTIQDVWTPIDHLAIVWCKQLREIISRYLVD",
    "response#3": "QLDGIPVLFIPGNAGSYRQVRSIAAACSELYFKQSDILINKNAKNLDFFAADFNEDFTAFHGGTMLDQAEYLNDAIRYILSLYDQPDVSTTLAKPKSVIIVAHSMGGIVARLMPTLKNHIHGSVHSYLTLSSPHAAAPITFDGDVLQLYKRTNEYWKRELNDKSSFIFNNVSLISITGGIQDTILPADYAMIEDLIPYSNGFTVHTNTIQDVWTPIDHLAIVWCKQLREIISRYLVV"
  },
  {
    "reference": "MPKVSLFNQTGSQVGEIELADNVFGVEPNESVLHDAVVMQQASLRQGTHKTKGRSEVRGGGRKPWRQKGTGRARQGSIRSPQWVGGGVVFGPTPRSYSYKLPKKVRRLAIKSALSSKVKDSELVVLDDLKLEAIKTKAMKDVLASLSVDSKALVVTADYNENVALSARNLPGITFLTADGVNVLDLLKHDKLVITKDAVEKVEEVLA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that localizes to ribosome, ribonucleoprotein complex and can perform rRNA binding, structural constituent of ribosome with high specificity and efficiency.2. For general function, the protein need meet that Forms part of the polypeptide exit tunnel.3. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "MSVGVGMRADKDGDKVVFKVGFSHSVTVAPPAGITLSVDGTTKVKVSGINKEDVGQMAAEIRSIRKPDHYMGKGIRR",
    "response#2": "EPIGCHTTLRGDRMWEFLDRLLSLALPRIRDFRGLSPKQFDGNGNYTFGLNEQSMFHEIDQDKIDRVRGMDITVVTTATTDDEGRALLRALGFPG",
    "response#3": "MEPIGCHTTLRGDRMWEFLDRLLSLALPRIRDFRGLSPKQFDGNGNYTFGLNEQSMFHEIDQDKIDRVRGMDITVVTTATTDDEGRALLRALGFPQ"
  },
  {
    "reference": "MTTTNRLRIALQKKGRLSKDCNELLKQCGVKINWNEQRLIAYAENMPIEILRVRDDDIPGLVFEGVVDLGIIGENVLEEEELGRLARGEKIEYKKLRTLDFGGCRLSLAIDRDRTYNGVQDFVNSRIATSYPNLLKRYMNEKGVAFKSTLLNGSVEVAPSAGLADAICDLVSSGATLEANGLKEVEVIYQSKACLIQRAEPLSTEKQALVDRLLTRIQGVQQAAESKYIMLHAPKDKLKEITALLPGVENPTILPLANDSARVAMHVVSQENLFWETMEQLKEMGASSVLVLPIEKMLA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.2. We need a protein that can perform 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-   alpha-D-ribose 1-diphosphate + ATP with high efficiency.3. The protein must exhibit the following characteristics: Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity..4. The designed protein should be optimized for ATP phosphoribosyltransferase activity, magnesium ion binding, ATP binding activity in order to improve histidine biosynthetic process.5. The protein design should consider the endogenous proteins and enzymes present in the L-histidine biosynthesis, L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9, Amino-acid biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "IMFALPNKGRISEPVMKVLEKAGLKITVKGRSLFANTVDDNIKVMFARARDIPEFVADGVADIGVTGYDLVLERNVEDKVDFLLDFGFGFAKLVLAAPESSNINSIDDIKEGMRVATEFPNLTKKYFEKLNKKVEIIELSGATEIAPFIGIADLISDLTSTGTTLRLNRLKVIDEIVSSTTRLIRPKDIATYVGSGDLDVGITGRDLLVDSGAPATEVVDLGFGQATFRFAARPEDIDTAQDLDGRRVATAYPGLVERHLAELGVKADVIRLDGAVENAIRLGLADVVADVVETGATLRQAGLVVFGEPLLRSSAVLVCRSAAQPHPQGELLLRRKPMDVVTYVEHGVADLGIAGKDVMMEEERDVYELLDLKISKCHLAVAGLPGVRMNQIAPRVATKYPNIASSYFREQGEQVEIIRLNGSIELAPLIGLADRIVDIVSTGRTLKENGLVELEKIADVTSRLIVNPVSYRMKDEAVDELVHRLELSNIKQSLINKLIIRIQGVIQARGSKYIMLHAPAEQLEEIINLLPGAESPTVLPLAGNQHRVAIYMVSNETLFWETMENLKNLGASSILVLPIEKMFS",
    "response#2": "IMFALPNKGRISEPVMKVLEKAGLKITVKGRSLFANTVDDNIKVMFARARDIPEFVADGVADIGVTGYDLVLERNVEDKVDFLLDFGFGFAKLVLAAPESSNINSIDDIKEGMRVATEFPNLTKKYFEKLNKKVEIIELSGATEIAPFIGIADLISDLTSTGTTLRLNRLKVIDEIVSSTTRLIDEVAPRAGLSDGICDLVSTGATGAETKRLIMMNAPKDKVEEIRKLIPGMAGPTVSKVLSDDNMVAIHAVVNEDEIFTLVPKLHALGARDILVVPIER",
    "response#3": "IMFALPNKGRISEPVMKVLEKAGLKITVKGRSLFANTVDDNIKVMFARARDIPEFVADGVADIGVTGYDLVLERNVEDKVDFLLDFGFGFAKLVLAAPESSNINSIDDIKEGMRVATEFPNLTKKYFEKLNKKVEIIELSGATEIAPFIGIADLISDLTSTGTTLRLNRLKVIDEIVSSTTRLIEKPMDVVTYVEHGVADLGIAGKDVMMEEERDVYELLDLKISKCHLAVAGLPGVRMNQIAPRVATKYPNIASSYFREQGEQVEIIRLNGSIELAPLIGLADRIVDIVSTGRTLKENGLVELEKIADVTSRLIVNPVSYRMKDEAVDELVHRLNKKSLKEKGKILDIKTALESVLNAKKKRYLMMNVPEASLQAVKEVLPGMSGPTVMKVESSRSSEESFLAVHSVVDADLIFTIVNKLKNVGARDILVVPIERII"
  },
  {
    "reference": "MKKILIPMLALGGALAMQPALAQDGEALFKSKPCAACHSVDTKMVGPALKEVAAKNAGVEGAADTLALHIKNGSQGVWGPIPMPPNPVTEEEAKILAEWVLSLK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Design the protein with a signal peptide incorporated.2. The protein must exhibit electron transfer activity, heme binding.3. The designed protein should have a high specificity for heme c binding, with minimal off-target effects.4. The intended protein structure should align with helix-helix-strand-strand-helix-strand-helix-helix-strand-helix criteria. The designed protein sequence is ",
    "response#1": "AVVGLAGITFANEQLAKQKGCMACHDLKAKKVGPAYADVAKKYAGRKDAVDYLAGKIKKGGSGVWGSVPMPPQNVTDAEAKQLAQWILSIL",
    "response#2": "AVVGLAGITFANEQLAKQKGCMACHDLKAKKVGPAYADVAKKYAGRKDAVDYLAGKIKKGGSGVWGSVPMPPQNVTDAEAKQLAQWILSILG",
    "response#3": "VVHAIDTRTGNRIWTYDPQIDRSTGFKGCCDVVNRGVALWKGKVYVGAWDGRLIALDAATGKEVWHQNTFEGQKGSLTITGAPRVFKGKVIIGKRGAEYGVRGYITAYDAETGERKWRWFSVPGDPL"
  },
  {
    "reference": "MADFMDIEPSSHSAKASQYESSAPASSSLGNSHPNESLDYYIYDYFVKHNFEEAAQAFLRESKIQIPKSSSSTAFSPSNNNAPSPFPPKNSSLASPSKISESISGDRLYNHMSSAPSPNKKEETNVVHANEDISLDKRQSFGSSSLPPSEVSINVPEGFLVEWFNIFWDVFSARVSRVNSTPIQLYDPSTQRQMARPMSNLQASQPVPSSTFSRSAVVPNPSLPLNPSVLQGQVMNNPTIPKGTPSTSIEGAKTSIPPSHAMQNPHNSFPASADRLQKNHPVQSSNFNPYTPAPSITVPPNYIPNTAMMGPSYSSFGDTDPRTYPAGMGPNPTAARNGFYPPTPAQIHQLKAQQQHLQRQSKQMSEPAPINMKSNKDQQLQYVDFRGVGSGADLQKQQWNKSTSAEGLQPNGLVMRNFGDVRHQKLPTSSPPSQHPPVGQIPSQYLPYQAGLKVPGNTPIPVKQVGGMPLQSPLPVSMKPSADDHSRATPTRSVEAPTLPSYAPRHPTQANGSRYMNPSTSRMTPQSPYMQNYYRPHAQMQDQNNMMSYMLSQQKAMEIAKSREMAIQRNTQTLSSGNQPPQQSGPNPNEFSMSMDPANMQQGNHALSDYHMQLMLLEEQNKKRLMMARQEQGTGSLSPQSYMNSRYSVDVGKEHMSMIPNQTAPMIQPNVPVSANSPAQANTPAADSTKSGTIQPTNRNGEGLSYSPHQQFSPSAPQAEKLSRSMSPFVSQQQQLNQPVSNKPDGLTQNKEVTGMPLNKEELTNPAF",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a specific function within the nucleus. The designed protein sequence is ",
    "response#1": "PRPCRVLVLLNPHSGKGKALQLFQSRVQPLLVEAEISFMLMLTERQNHARELVRSEQLGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQMPLCSLPAGSANALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTAV",
    "response#2": "NLSLQPQKYNSIAEARGKIYYLAKDQHGCRFLQRIFSEKDGNDIEMIFNEIIDYISELMMDPFGNYLVQKLLEVCNEDQRMQIVHSITRKPGLLIKISCDMHGTRAVQKIVETAKREEEISIIISALKHGIVHLIKNVNGNHVVQRCLQYLLPYCGKFLFEAAITHCVELATDRHGCCVLQKCLGYSEGEQKQHLVSEIASNALLLSQDPFGNYVLQYVFELQLQWATFEILEQLEGNYTELSMQKCSSNVVEKCLKLADDKHRARIIRELINYGRLDQVMLDPYGNYVIQAALKQSKGNVHALLVDAIKLNISSLRTNPYGKKVLSAC",
    "response#3": "PRPCRVLVLLNPHSGKGKALQLFQSRVQPLLVEAEISFMLMLTERQNHARELVRSEQLGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQMPLCSLPAGSANALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTAR"
  },
  {
    "reference": "MDLLHKAKLAPLLPGVYIFYGKNKEYIYVGKAKRLRNRLLSYFNRSNGKYSKKIQAIVNEAEELDYIVVSNEREALLLEANLIFNHKPKYNVMLKDAEFYPYIEITKELFPAVQIVRIRSTGGEYFGPYTDVKFVKDLIDCLQQVYQFRTCRRDMSKSTKPCMEFYMHRCAAPCTGDLEPDSYINSSIQPLRRVLNGDISETLDLIEEKMKKHAKMMDFENAAKYRDLLVKFENVMQRQGVVLEQWRNLDVIGRFKNSYAVLRIRGGHVVGKLSYELDSTKLEDFIFHYYMVGKNELPEAVILERNINFDAEIYFGRPRDKLEEELLHKARENAKNQAYTSGLRKDLLNKMVKVLNLNRYPMKIEGFDVSHLHGKLTVASVVVFFDGLPRKNEYRHYRFNSDRIDDFLTLKELVKRRYSKHELPDMIFVDGGTGQINAVVEALMEIGKECDVVGLAKQNEIVCTRFGELILPFDSPILRTLVRIRDEAHRFANSFHRKLRRKSALSSILDEIPGIGPKRKKKLIEAFGSVKNIRSATLQEIAEVLGSRKLAAEILSRL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be involved in nucleotide-excision repair by localizing to cytoplasm, excinuclease repair complex and exhibiting DNA binding.2. The protein must exhibit the following characteristics: UvrC both incises the 5' and 3' sides of the lesion.3. The presence of GIY-YIG domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MFMRGGKIISSAFEKIHSLNGFDTDEAMKQAIINHYQSHLPLMPEQILLNACSNETLKELQEFISHQYSKKIALSIPKKGDKLALIEIAMKNAQEIFSQEKTSNEDLILEEARSLFKLECMPYRVEIFDTSHHSSSQCVGGMVVYENNAFQKNSYRRYHLKGSDEYTQMSELLTRRALDFAKEPPPNLWVIDGGRAQLNIALEIL",
    "response#2": "MFMRGGKIISSAFEKIHSLNGFDTDEAMKQAIINHYQSHLPLMPEQILLNACSNETLKELQEFISHQYSKKIALSIPKKGDKLALIEIAMKNAQEIFSQEKTSNEDLILEEARSLFKLECMPYRVEIFDTSHHSSSQCVGGMVVYENNAFQKNSYRRYHLKGSDEYTQMSELLTRRALDFAKEPPPNLWVIDGGRAQLNIALEIL",
    "response#3": "FVSIYGRSGTYCLQVISFRGGISYGSQPYFVDDGNYESESDVLGMFMLQVYSDPPGRVYVDCESDYCEVINAALERLLTRKVDILTAKSHEELKFLRLARNSAMEALNRRLRDKALPAELEELAELFGLPNPPERIEVYDNSHISGTHPFGVMVVCGKDGLLRKEYRKFKIQTVLNGDDYSMMHEVLFRRFSEESPSVPDFVLIDGGRGHISSAIQVL"
  },
  {
    "reference": "MNIVVLGPGAVGSLWALHLHSAGHQVALWSRQAQPTITLQLDEEAPISFRNQNLDTLIHADLLLITVKAWQVEAALQPLLPHLNRETILLFMHNGMGAVEAISESLTHFPVLFATTTHGALKATLNQVSHTGFGQTQVGPFNALGARCDFIADVFNHALAPVTWNPEIQQALWRKLAVNCAINPLTAIHQCANGALVAPEFTPIITAILDEVTAVMQAEAISGEAEALRDGVYQVIQATAANLSSMHQDVFHRRPTEIDFITGYVVRKGEQHGIATPVNSALYQQIKTLEQSWSKA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be optimized for NADP binding, 2-dehydropantoate 2-reductase activity.2. The substrate, NADP(+) binding site should be stable and able to withstand changes in environmental conditions.3. For general function, the protein need meet that Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.4. A key design requirement for the protein is the optimization of the Proton donor to achieve high catalytic efficiency for (R)-pantoate + NADP(+) = 2-dehydropantoate + H(+) + NADPH.5. The designed protein should interact with enzymes and substrates within the (R)-pantothenate biosynthesis, (R)- pantoate from 3-methyl-2-oxobutanoate: step 2/2, Cofactor biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MDIEPYRWLKLAVNAAINAITALLQAPNGVIISSPYAQTLALEVAQEVLNVATALGVKMPRNPVEEVLRVASATAKNLSSTARDVAACAKTEIDYINGAVVKYGEALGVATPVNKALFNLIKARNQCEKLVINACINPTTATLDCVNGELYNDESAKELFRCIIKECVDIFFKCIPLFKNNEEAEKILNVNRLLDRVMFVGTKVNGANSSSTRQDCLLLRETEIDAINGYVVKLAENNGFQATVNKTMMLLTA",
    "response#2": "MDIEPYRWLKLAVNAAINAITALLQAPNGVIISSPYAQTLALEVAQEVLNVATALGVKMPRNPVEEVLRVASATAKNLSSTARDVAACAKTEIDYINGAVVKYGEALGVATPVNKALFNLIKAREGSC",
    "response#3": "MKNIISWIWAKAIVNSAINPIGTLLEVKNKVIRENDFLLSMAMEVVKEGCRVALQNGIEFDVPPMDLFFQTLEQTRENYNSMLQDIWRGKKTEVDYINGKIVEYAKAVNLEAPMNLLLWGLIKGKNQCEKLVINACINPTTATLDCVNGELYNDESAKELFRCIIKECVDIFFKCIPLFKNNEEAEKILNVNRLLDRVMFVGTKVNGANSSSTRQDCLLLRETEIDAINGYVVKLAENNGFQATVNKTMMLLTT"
  },
  {
    "reference": "MSGNSIGQNFVVTTFGESHGVALGCIIDGCPPGLELTEADMQHDLDRRRPGTSRYTTARREPDEVRILSGVFEGKTTGTSIGLLIENTDQRSQDYSNIKDLFRPGHADYTYQQKYGMRDYRGGGRSSARETAMRVAAGAVAKKYLKQVHGIEIYGFMSQLGPICAETIDLDQIEQNAFFFPDASKLEALDEYMRELKKSGDSIGAKISVIATGVPVGLGEPVFDRLDADIAHALMGINAVKGVEIGDGFGVVTQKGSEGRDLMSPQGFESNHAGGVLGGISSGQPIIAHIALKPTSSISVPGQSMTAQGEMAEVVTKGRHDPCVGIRAVPIAEAMLAIVLMDHLLRHRAQNQDVRSHTPVLGMR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that This reaction introduces a second double bond into the aromatic ring system.2. The designed protein must be optimized for chorismate synthase activity.3. A protein that is capable of catalyzing reactions within the Metabolic intermediate biosynthesis, chorismate biosynthesis pathway with high efficiency and specificity.4. We are looking for a protein with broad substrate specificity for 5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate +   phosphate. The designed protein sequence is ",
    "response#1": "MGSMIGNAMGG",
    "response#2": "MXVVXPXRXP",
    "response#3": "LFPGKK"
  },
  {
    "reference": "MRGVIRKLNDDGFGVLKGILVPFSAPGDEIIVERVERVKKRRVASQWKLVRSSPLRVGPRCKAFGKCGGCTLQHLNYDYQLEFKRKKLKRILGFEVEVVPSPKIFGHRNRIDLAITKDGIGFRERGKWWKIVDIDECPVFGKTSREAIERLKEFIEEEKISVWNIKKDEGFLRYMVLREGKFTEEVMVNFVTKEGNLPDPTNYFDFDSIYWSVNRSKSDVSYGDIERFWGKEFIRERLDDVDYLIHPNSFFQTNSYQAVNLVRKVSELVEGEKILDMYSGVGTFGIYLAKRGFNVKGFDSNEFAIEMARRNVEINNVDAEFEVASDREVSVKGFDTVIVDPPRAGLHPRLVKRLNREKPGVIVYVSCNPETFARDVKMLDYRIDEIVALDMFPHTPHVELVAKLV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in tRNA.2. The protein must be able to metal ion binding, 4 iron, 4 sulfur cluster binding, S-adenosylmethionine-dependent tRNA (m5U54) methyltransferase activity.3. The protein with the reaction S-adenosyl-L-methionine + uridine(54) in tRNA = 5-   methyluridine(54) in tRNA + H(+) + S-adenosyl-L-homocysteine, the Proton acceptor must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "FSYGHSVCVTTQVGCNIGCSFCASGLLRKSRDLKAGEIVEQIMNVQEYLDGMGEGARVSNIVVMGIGEPFDNYDNLMAFLRIVNDGDGLSIGARHITVSTSGLVKGIRAFADEDLQVNLALSIHAPNNELRTRIMKINKAFPLEQVMDAIDYFLSKKNRRITFEYILLDGVNDHVEEAVQLAKLLQNKKHLSYVNLIPYNPVDEHDQYKRSSKESIDKFEKALKQKGIQCGVRLEHGTGQRG",
    "response#2": "TTQVGCNIGCSFCASGLLRKSRDLKAGEIVEQIMNVQEYLDGMGEGARVSNIVVMGIGEPFDNYDNLMAFLRIVNDGDGLSIGARHITVSTSGLVKGIRAFADEDLQVNLALSIHAPNNELRTRIMKINKAFPLEQVMDAIDYFLSKKNRRITFEYILLDGVNDHVEEAVQRYM",
    "response#3": "TIHKENGVLIKVDLSKVFFNPRMKGERYRIAQLVNDGERILVPFAGVIPYPLVIARFKNVEVYAVEINEFAVKLAEENLELNRDRLKGKIKIIHGDVFEVLPNLPNFDRVVSPTPKGVDALSLTLSKAEKFLHYYDFVHESEIERFRERVLEECRRQGKELDIVGDIAIVSIPDEILSEREVIVSAIRKLYPKVKVIARRGFHSGLYRIRELEVIWGENRLHTIHKENGVLIKVDLSKVFFNPRMKGERYRIAQLVNDGERILVPFAGVIPYPLVIARFKNVEVYAVEINEFAVKLAEENLELNRDRLKGKIKIIHGDVFEVLPNLPNFDRVVSPTPKGVDALSLTLSKAEKFLHYYDFVHESEIERFRERVLEECRRQGKECRVSVRKVSDYKPHVYKVCADVEILK"
  },
  {
    "reference": "MLMPKKTKYSKMMKGRNRGYAVRGIKLNFGDYGIKAVEAGRINSNQIEAARIAMTRYVNREAKVWINVFPDKPLTKKPLQTRMGKGKTGVEEWVMNIKPGRIIFELTGVNEDVARRALELSMHKLPFKTKIVTRESENEIY",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a specific function within the ribosome. The designed protein sequence is ",
    "response#1": "EIDVHVPIHLVGEAAGVKDGGVLQQAMHQLSIRVLPSNIPPSIDVDITNLQVGDVITVSHIQTNGAYEINHDPTEVIASILPPV",
    "response#2": "EIDVHVPIHLVGEAAGVKDGGVLQQAMHQLSIRVLPSNIPPSIDVDITNLQVGDVITVSHIQTNGAYEINHDPTEVIASILPPL",
    "response#3": "MRLQLQLRTDHRRSYVKQMRKQGHIPAVLYGKTTNSQPVFVDAAEFLKQLRATGGTGLLDAKMNDNHIRIIVRDMQKHPIRHDIIHIDFLN"
  },
  {
    "reference": "MTRYIFVTGGVVSSLGKGIASASLAAILEARGLKVTMLKLDPYINVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTTMSKRNNFTAGRVYQHVLRKERRGDYLGGTVQVIPHITDEIKRRVLEGAGDADIALVEIGGTVGDIESLPFMEAVRQLKVELGARRAMFMHLTLVPYIATAGEIKTKPSQHSVKELRSIGIQPDILVCRSDFALPVSSRRKLSLFTNVEERAVISLPDADCIYKIPRMLSEQKLDQIVIEQFNLDCPPADLTEWDRVSDAKLNPHREVTIAMVGKYMELLDAYKSLIEAISHAGIALRTKVNIKYIDSEQIERDGIGLLEDVSAILVPGGFGERGVEGKIAAVKFARENKVPYLGICLGMQVAVIEYARHVAGITDASSTEFDANAASPLVGLITEWMTEEGAREIRGYTDDLGGTMRLGAQVCTLKEGTRAYQAYGATEIKERHRHRYEVNNNYVPQLEEAGLIISGRSVDGELVEVVEAPDHPWFVACQFHPEFTSTPRDGHGLFTGFIEASLAYQDLEK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A key design requirement for the protein is the optimization of the Nucleophile, Nucleophile; for glutamine hydrolysis, active site to achieve high catalytic efficiency for H2O + L-glutamine = L-glutamate + NH4(+).2. The designed protein should be able to compete with endogenous proteins or inhibitors for L-glutamine, Mg(2+), UTP, ATP, CTP binding.3. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.4. A protein should be able to manipulate the activity of enzymes in the CTP from UDP: step 2/2, CTP biosynthesis via de novo pathway, Pyrimidine metabolism pathway, either by activating or inhibiting their function.5. The designed protein have Glutamine amidotransferase, CTP synthase N-terminal domains with specific sequence motifs that are crucial for its activity.6. The designed protein should have ATP binding, metal ion binding, CTP synthase activity, glutaminase activity to facilitate glutamine metabolic process, 'de novo' CTP biosynthetic process. The designed protein sequence is ",
    "response#1": "MATKYIFVTGGVTSSLGKGIISASLGKLLQARGFRVTIQKFDPYINIDPGTLNPYEHGECYVTDDGAETDLDLGHYERFLNICTSQANNVTTGRIYNNVITKERKGEYLGKTVQVVPHITDEIKRNIFLLGESGEYDFIITEIGGCVGDIESLPFLEAVRQVRWDLGPNNALVVHLTLIPYLKAAKELKTKPTQHSVKQLLEAGIQPDVLACRTERPLPLELRKKLALFCNVHLNSVIEAIDADSIYDVPLLMKKEKLDERVLAKLRDADKSLCEALTHGGIANDTRVNIEWIEAESFEKEPHTVAERLKDKKAILVPGGYGERGTAGMISAIQYAREHQIPYMGICFGMQLAVIEVARHLAGIEKANTTEFGVTAEPVVGLLTEWLNGDVKERRSAEDDLGGTMRLGLYPAILAPGSLIQKIYGTSSIQERHRHRYELNIEYREKFEKAGLKFSGLSPDGLLPETVELEGHPWFVGVQFHPELRSRPFDPHPLFTSFIDSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASFVSAFEKQAHEVKIAVVGKYVDLKDAYLSLSEALCHGGIAHNTAVEIVWVDAEQVEQGDLGEIFSGVSGILVPGGFGGRGVAGKIRAINYARTRGVPFLGICMGMQCAVIEFARNVCGLKAANSTEFDPETPYPVIDLLPDQRNIEDLGGTMRLGLYPCRLLPGSRAAQAYGVEEVKERHRHRYEFNNRFRDLLEEKGLKVTGTSPDGVLVEVVEIPDHPWFLACQFHPEFKSRPTRPHPLFRAF",
    "response#2": "KYIFVTGGVVSSLGKGIAAASIGCLLESRGLRVTLQKFDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTYAPLTQSNNVTSGRIYETIISRERRGDYLGKTVQVIPHVTDEIKANIEKVSGGVDVVLVEIGGTVGDIESLPFLEAIRQLRHKKGRENCFFVHVTLVPWIAAAGELKTKPTQHSVKELRTIGIQPDMLICRCEKPLAGDIKDKIALFCDVSADAVIEACDAKSVYEVPLGFASQNVDDIILRQFKLPVNKPKLDEWKEFVKTIESPKGEITIGLVGKYVELKDAYKSISEAIIHAGAKNSCKVNIEWLHAEKINTNNVKQKLSKLKGIIVAPGFGDRGIEGKITAIKYIRENNIPFFGICLGMQSAVIEYARNVLGLRNANSTEMDSKTSXPVIDLMNEQKEVTKKGGTMRLGTYDCYLKNNSNAYNAYKNNNIKERHRHRFEFNNKYKNDLEKAGLIATGINKNKNLVEIIEIAQHPWFVGVQFHPEFKSTVDNPHPLFCGFIKAALKYKA",
    "response#3": "KYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTHLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTSAKMGKRNNFTTGQIYESVIKKERRGEYLGKTVQVIPHITDEIKLYLKRGAEGSDVAIVEVGGTVGDIESLPFLEAIRQMGLEEGRQSSCFIHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDILLCRADRSVPADERRKIALFCNVMPEAVIECLDADSIYRIPGMLHDQMLDEIVKHIFVTGGVVSGLGKGITAASLGRLLKCRGYKVAAQKLDPYMNVDPGTMSPLQHGEVFVTEDGAETDLDLGHYERFIDEDLNKYSNLTSGKTYWTVLQKERNGEYLGSTVQIIPHVTNEIKSFIYALADKTKADVVITEIGGTTGDIESQPFLEAIRQISHEVGKENCLFIHVTLVPYIKGSEEHKSKPTQHSVKELMGSGIFPDIIVCRSDEKIEESVLNKIALFCNVKKDCVIENITVPVLYEAPIMLSKQGLDRVALRELGLPVDRKPDWRDWEELLRRVDSPQRELPDSYKSINEAFVHAGAQNDCKVNVKWISSEEINPETCEDILAGLDGILVAPGFGERGMEGKIEAIRYVREQKIPFFGVCLGMQCAVIEFARNVLKLHGASSTELDPNTRHPVIDLMEEQKKVTEKGGTMRLGAYACRLKKGSKAYNIYGESIIHERHRHRYEFNNKYLNDIEEAGLKAVGVNPESGLVEVVELKDHPWFIGVQFHPELKSTVLNPHPLFVKFVFLKAATG"
  },
  {
    "reference": "MITCLRNWPLLLKRFSVHQIHQFTQLSGRFERPPQSGKSSRDPYLVTVVQGRSKKPRFPGERSNQRFGEDSWFVSSTPLAEVMGVADGVGGWRDLGVDAGRFAKELMSCCSGQTQLSDFDGRSPRNMLIAGFQELSHREHPVVGSSTACLATMHRKDCTLYTANLGDSGFLVVRNGRVLHRSVEQTHDFNTPYQLTVPPEDRKESYYCDKPEMAVSTRHSLLPGDLVLLATDGLFDNMPESMLLSILNGLKERGEHDLLVGASRVVEKARELSMNASFQSPFAIKARQHNVSYSGGGKPDDITLILSSVEVPNA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein have enhanced H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +   phosphate.2. The designed protein should be able to compete with endogenous proteins or inhibitors for Mn(2+) binding.3. The presence of PPM-type phosphatase domains is critical for the interaction of the designed protein with its intended targets.4. The protein requires Mg(2+), Mn(2+) to activate a critical catalytic site.5. A protein that contributes to protein dephosphorylation, dephosphorylation, regulation of ubiquinone biosynthetic process by functioning in mitochondrion with metal ion binding, phosphoprotein phosphatase activity, protein serine/threonine phosphatase activity. The designed protein sequence is ",
    "response#1": "KYASSAMQGLRMSMQDALAVELDLDALKSTSFFGVYDGHGGAEVAMYCAKRFHVMLREEESFLNNLSYAITSVCSRLDDELEAPNVWRASLYPHRSSESSSESSDCFQFLSTGSCANVWRSSEAVSYKLPSYEGSTACVVIIRGNQITVGNVGDSRCVLSKNGQAIDLSTDHKPNVPLERQRILRVGGQVWREKFPAKDSGGEIREQWGPYCIEGKLSTSRALAGIFLTTISGDFAYKNIVYRPQYQMVTHFPDIRVAKITGDTEFLVIASDGICSIQILIVDLNTFFPFRDHMSSQDVVDFVHEKLNSRRQELCQSLINQGKKRECFTEDSQLATNKNIAPNTTTLGEETLHTTCEKLVENCLESRNNATAILVQFMRDHN",
    "response#2": "LVIGALENAFQECDDVIGRELEASGQVGGCTALVAVFLQGKLYVANAGDSRAILVRRHEIRQLSSEFTPETERQRIQQL",
    "response#3": "PSCGRTEEFPRYGVTAVCGRRREMEDAVSIRPDFLPASGKFHFYGVFDGHGCSHVATTCQDRMHEIVAEEHNKGASGEVAPWRDVMEKSFARMDGEVGNRASTRSDDEPACPCEQQTPSRRDHAGSTAVVAVVSPTQVVVANAGDSRAVISRAGVPVALSVDHKPDRPDELERIEAAGGRVIYWDGARVLGVLAMSRAIGDGYLKPYVTSEPEVTVTERTDDDECLILASDGLWDVVTNEMACEVVRACFHNNGPPAPAARPSGVPSSAEAAETENGGAASVKGISKAESSDKACSDAAMLLTKLALARRSADNVSVVVAGEDRVHVVVSEDQRWLFVGIYDGFNGPEAPDFLVTNLYRFLLRELRGIFYKEADADNKKLWQFLVDGDDDDSELDFSGSGRFALSLDRLKESRFHMWAHAAADESGREWGSRRLAPAPAVRDHAAVLAALTRALASTEAAYLDMTDQSMGTHPELAVTGACLLVALVRDDNVYVMNLGDSRAIVAQRPDDGDDGCVFGTMRRMEDVGVGLEIETRPGGCAIIGLKPLQLSTDHSTSIEEEVHRIKREHPDDDQCIVNDRVKGRLKVTRAFGAGYLKQAKLNNGLLEMFRNDYIGDTPYISCTPSLCHHKLTARDQFLVLSSDGLYQYLSNEEVVLHVENFMERFPEGDPAQSLIEELLSRAAKKAGMDFYELLDIPQGDRRKYHDDVTVMVISVLG"
  },
  {
    "reference": "MLEKESDLTAMEKPNNKHAADSFSSEDLVSPVKKAKKSEEVSGGGEAVAAVGNREAEEDKPSFVSEEKKEFLVEADVAEDKGARHTMEDVWVVLPDASLDFPGTLRCAHFAIYDGHGGRLAAEFAKKHLHLNVLSAGLPRELLDVKVAKKAILEGFRKTDELLLQKSVSGGWQDGATAVCVWILDQKVFVANIGDAKAVLARSSTTNELGNHTEAGNPLKAIVLTREHKAIYPQERSRIQKSGGVISSNGRLQGRLEVSRAFGDRHFKKFGVSATPDIHAFELTERENFMILGCDGLWEVFGPSDAVGFVQKLLKEGLHVSTVSRRLVKEAVKERRCKDNCTAIVIVFKRV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. We need a protein that can perform H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +   phosphate with high efficiency.2. The protein must be engineered to have a high affinity for Mn(2+), Mg(2+) in order to increase its catalytic activity.3. The designed protein should contain one or more PPM-type phosphatase domains that are essential for its function.4. The protein design must be able to perform myosin phosphatase activity, metal ion binding in order to contribute to protein dephosphorylation.5. The designed protein should have a high specificity for Mn(2+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "LRRDDGVQWAHGRAGEDRVHVVVSEDQRWLFVGIYDGFNGPEAPDFLVTNLYRFLLRELRGIFYKEADADNKKLWQFLVDGDDDDSELDFSGSGRFALSLDRLKESRFHMWAHAAADESGREWGSRRLAPAPAVRDHAAVLAALTRALASTEAAYLDMTDQSMGTHPELAVTGACLLVALVRDDNVYVMNLGDSRAIVAQRPDDGDDGCVFGTMRRMEDVGVGLEIETRPGGCAIIGLKPLQLSTDHSTSIEEEVHRIKREHPDDDQCIVNDRVKGRLKVTRAFGAGYLKQAKLNNGLLEMFRNDYIGDTPYISCTPSLCHHKLTARDQFLVLSSDGLYQYLSNEEVVLHVENFMERFPEGDPAQSLIEELLSRAAKKAGMDFYELLDIPQGDRRKYHDDVTVMVISSRGVMVGMGQKDSGLDDIINRLLEFRNARTVRQVQLSEAEIRSLCTASKEIFLHQPNLLELLDDIINRLLEFRNARTVRQVQLSEAEIRSLCTASKEIFLHQPNLLELREKMTQIVFDSTRSAMGKDHGQGRQARLLGTLAVSRGLGDHQLRVLDTDIQLKPFLLSIPQVTVLDVHQLAVQEEDVVVMATDGLWDVLSNEQVALLVRSFLTGNQKDDPHRFSG",
    "response#2": "LSETEIKQLCFVSRDIFLRQPNLLELEAPVKICGDIHGQYPDLLRLFEHGGYPPNSNYLFLGDYVDRGKQSLETICLLLAYKIKFPENFFLLRGNHESASINRIYGFYDECKRRFSVKIWRIFTDCFNCLPVAALIDERIFCMHGGLSPELLSLRQIRDIRRPTDIPDRGLLCDLLWSDPDKDVRGWGPNDRGVSYTFGSDIVSGFLKRLDLDLICRAHQVVEDGFEFFANKQLVTIFSAPNYCGEFDNAGAMMSVSEDLTCSFQILKSNPVKICGDIHGQYPDLLRLFEHGGYPPNSNYLFLGDYVDRGKQSLETICLLLAYKIKFPENFFLLRGNHESASINRIYGFYDECKRRFSVKIWRIFTDCFNCLPVAALIDERIFCMHGGLSPELLSLRQIRDIRRPTDIPDRGLLCDLLWSDPDKDVRGWGPNDRGVSYTFGSDIVSGFLKRLDLDLICRAHQVQDGDTDFACKKNERLPPEDQMLTCNPDILTMDITDDMEFLVIATEGLWCNMTNQNVVDHTHDRLLEGAEARVICEELVQFGLPSDRPHAAGEFSMAAAQANLAMEDQAQVLASPAATLVGVYDGHGGADASRFLRSRLFPHVQRFEKEQGGMSTEVIRRAFGAAEEEFLQQVRQAWRQRPKMAAVGSCCLLGAISGDTLYVANLGDSRAVLGRRVVGGGVAVAERLTDEHNAASEEVRRELTALNPDDAQIVVHARGAWRVKGIIQVSRTIGDVYLKKQEYSMDPVFRNVGPPIPLKRPALSAEPSIQVRKLKPNDLFLIFASDGLWEHLSDDAAVQIVFKNPRTGIANRLVKAALKEATRKREVSFRDLKTIEKGVRRHFHDDISVIV",
    "response#3": "DQEWLTVCPEEFLTGHYWALFDGHGGPAAAILAANTLHSCLRRQLEAVVEGMIAPQPPMHLSGRCVCPSDPQFVEEKGIQAEDLVIGALENAFQECDDVIGRELEASGQVGGCTALVAVFLQGKLYVANAGDSRAILVRRHEIRQLSSEFTPETERQRIQQLAFTYPELLAGEFTRLEFPRRLKGDDLGQKVLFRDHHMRGWSYKRVEKSDLKYPLIHGQGRQARLLGTLAVSRGLGDHQLRVLDTDIQLKPFLLSIPQVTVLDVHQLAVQEEDVVVMATDGLWDVLSNEQVALLVRSFLTGNQKDDPHRFSELAKMLIHNTQGKDNGATGEGQVSYDDVSVFVIPL"
  },
  {
    "reference": "MRDADADAGGGADGGDGRGGHSCRGGVDTAAAPAGGAPPAHAPGPGRDAASAARGSRMRPHIFTLSVPFPTPLEAEIAHGSLAPDAEPHQRVVGKDLTVSGRILVVRWKAEDCRLLRISVINFLDQLSLVVRTMQRFGPPVSR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in tRNA threonylcarbamoyladenosine metabolic process. The designed protein sequence is ",
    "response#1": "NAQLAKLWPLIFSSLGHEYDDVSQHVFPFIQQYLLLCKKSPELGSLDLLSTLLNKIIIKMKFDDDANGVDDDDETEQFSEIRSKLKNFQDTIAVLKPDLYVEAITIVINESLFGGDKNWRKIELGLFELNNFSESLRNNLINLPKSEITNSKPFQIFQEFLVKLINSDLIINVNHPKIQIGFFDIVVKHYNFLNAQSSQQSLILRILEIFTSPLGLFNENEKVRLRSWYSFFRFVKLTKPSLNNTAFVENVVIKLQPLLVIKAELPTTDEDDDVVENGNFSSQLYLYESIGLLISLLSTDLMNHKIKFIDLVFQPLFNDLENCVSSSEQVKLNQPLISMQAHHCLMALGTFARGYDHDFQNKYSEEIRGKINNAAQVVLITLENFPKNELIRDAARFSFARFIPILKNQINIHLAKFVTLVLAANNLKISELTDFLSFLGQIVHNYKR",
    "response#2": "NAQLAKLWPLIFSSLGHEYDDVSQHVFPFIQQYLLLCKKSPELGSLDLLSTLLNKIIIKMKFDDDANGVDDDDETEQFSEIRSKLKNFQDTIAVLKPDLYVEAITIVINESLFGGDKNWRKIELGLFELNNFSESLRNNLINLPKSEITNSKPFQIFQEFLVKLINSDLIINVNHPKIQIGFFDIVVKHYNFLNAQSSQQSLILRILEIFTSPLGLFNENEKVRLRSWYSFFRFVKLTKPSLNNTAFVENVVIKLQPLLVIKAELPTTDEDDDVVENGNFSSQLYLYESIGLLISLLSTDLMNHKIKFIDLVFQPLFNDLENCVSSSEQVKLNQPLISMQAHHCLMALGTFARGYDHDFQNKYSEEIRGKINNAAQVVLITLENFPKNELIRDAARFSFARFIPILKNQINIHLAKFVTLVLAANNLKISELTDFLSFLGQIVHNYKC",
    "response#3": "VSNFQGKPRPNAPYCWDAATMAETETLQKHWDLLPYLAENKLCDAGKDVSMGGIIGTALMLLETSKCGAILDLDAISCPAGLDFRQWLLSFPSYGFLLSVRPQFVKTVKACFQAEGLIAEAIATIQPGHNLTLY"
  },
  {
    "reference": "MAEGEITTFTALTEKFNLPLGNYKNPKLLYCSNGGYFLRIHPDGKVDGTRDRSDQHIQLQLSAESVGEVYIKSTESGQFLAMDANGLLYGSLSPSEECLFLERMEENHYNTYISKKHADKNWFVGLKKNGSCKLGPRTHYGQKAVLFLPLPVSAD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be involved in lung development, cellular response to heat, angiogenesis, regulation of endothelial tube morphogenesis by localizing to extracellular matrix, cytosol, nucleoplasm, extracellular space and exhibiting integrin binding, S100 protein binding.2. For general function, the protein need meet that Its binding to integrins and subsequent ternary complex formation with integrins and FGFR1 are essential for FGF1 signaling.; Acts as a ligand for FGFR1 and integrins; Functions as potent mitogen in vitro; Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades; Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration; Binds to integrins The designed protein sequence is ",
    "response#1": "MEENPTEDSAIPALDLNQTVQPEDPGNEERISPLDEDQEAEEKDFIMAPQNFPVWSVILLSVVTICSLVTAVFLYQLYTEDHSCPDGFIFKHKRCIPASVEALTMDQDQDKIPDTEEVLALYGTGYSDAESEDHGLSEGEDYPGRSLEDFNAEPPWNETENHQDYPDDSFRMEEDFPPMEEDFPPMEDDFPPMEDDFPPMEDDYPPLEDDFPPMEDDYPPLEDDFPPMEDDFPPLEDYFPPLEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDYPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPLMEDDFPPMEDGFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPPMEDDFPP",
    "response#2": "MDLFPRWSLLLLCLAVLAPRGSAANWVNWTVDQTQPGSTITLPCSHTFPWKDLTFAFWKKDSSGPILLHRYRDGQDQAKEGFKGRVSLLGDPTKGNASLLLQQLTREDSGTYRCEQGYGRSAYDTYLRVVPGSLESSYPLVTISPTSIQLSDTRGIKCRAEGEPKPEVTWSCRQGTCPEFQPDLGPVLGTGLTYQVNGTLEISSSQDSGVYKCTASNGVGQPAYALATLDVLYA",
    "response#3": "MLDCFEGQLCHVSCKLGLFQNCLYFAVFASLWLFWVLPVCRLLFGLSPWWNGWSWCWSWCWWWGWLLVLWWWWWWWWGWWWWWWWWWWWWWWLWWWWWGMMWWVWWGGSWWWWLWWWWWWWWWWWVAVAVWGWWWWWWWWWMWWWWWGWWWWWWWGVMWWWGVVLWGVVLWGVVLWGVVLWGVVLWGVVLWGVVLWGVVLWGVMLWGVML"
  },
  {
    "reference": "MAQAKTWVLKKHFEGFPKDSDFELKLEQLSEPRDGEVLLEALFLSVDPYMRPYSRVRMQEGDVMIGTQVAKVIKSNNHSFPVGCHVVGRCGWRTHTVSDGSDLTKVLDDWPQDVPLSHAVGAMGMPGLTALYGLEEVCAIKSGETLLVNAAAGAVGSVAGQIAKLKGCKVVGSAGSDNKVAYLKELGFDQAFNYKTVPSLEEALKKASPEGYDCYFENVGGPFSSVAIPQMKAFGRIAVCGGISLYNDSTPQTGPYPHMHMIFKQLKMEGFLVGRWEHKNEESLKRMLTWMQEVGGPFFTAALKNMKPGGRIAVCGAIATYNDTAPQMLVEKLFTRRILLPLLPEQPHSGYNHTLQVSCWGLWFGPQQRTMAQAKTWVLKKHFEGFPKDSDFELKLEQLSEPRDGEVLLEALFLSVDPYMRPYSRVSMQEGDVMIGAQVANDADIISSLRIRDLKLPSTASSWNLLLISLPLCSSLISLPLLCSPLSIYSLLSSSRRPRAACSSLATLPRRFCAALINPPLHSSQISSPLSSSSVPTPLPSCLVSASLPSRLQHRASALRCILLQCSCIIICPLHSDDLHSECVRCLGKSHTESALTEMICSHCERMSLASLCLCFSESDSAPRALLFSSSQGPVRKKQQGRGSQCSDETVRFSHPDQRPSAIASDLVLFGGSDEEPLDDSMSIAASDVEDALFMTPPPCRRWSQSMPGRPLTLNSPECCQRPSSSSGLEWVIKSNNLSFPVGCHVVGQCGWRTHTVSDGSDLTKVLD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.2. The protein should be designed to have high stability and activity towards leukotriene B4 + NADP(+) = 12-oxo-leukotriene B4 + H(+) +   NADPH.3. The presence of Enoyl reductase (ER) domains is critical for the interaction of the designed protein with its intended targets.4. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "PTNSDFELKTVELPPLKDGGKCSYILSMVLLCFRIMAKNLKEGDMMMGEQVARVVESKNAAFPPGTIVVAPSGWTTHSISNGEKLEKVLAEWPDTLPLSLALGTVGMPGLTAYFGLLDICGVKGGETVLVSAAAGAVGSVVGQIAKLKGCKVVGTAGSDEKVAWLKKHGFDVAFNYKTVKSLEEALKVAAPEGYDCYFDNVGGEFSNAVITQMKKFGRIAICGAISVYNRTGPLPPGPSPESIIIKELHLQGFVVYRWQGEVRQKALRDLLKGPGATVTPDALPCGGFRFHFHCEYGFIGKIQYHEHITEGFENMPAAFMGLLKGENLGKAIY",
    "response#2": "GMPKLSDFKIVEEEINEDLKDGEIIGEALYMSVDPFIRAYNVPPGSPVIGFQVAKVTKSKNPKYPVGSKVMAFGGWRTTCKVGDELIWYKVDETSGINASVFVGALGMPGMTAYCGFLEICQPKEGETVFVNGCAGAVGNLVGQIAKIKGCKVIGCCGSDEKIKFAKSIGFDDVFNYKTCKDYPGTLKKMAPNGIDCFFDNVGGQMSSDIMACMNVGGRIAVCGAITSYNSKTPSQVTMVQGYMVMKQLLMRGFLVHQFKEKFPEGLKNMTQWIKEGKIKIHETTTDKFENTPQAFIGMLSGKNIGKAVIE",
    "response#3": "GFPKISDMRLEEFDIPTLKDGEVLLEAEYLSVDPYMRPYSARLQTETVMIGQQVAKVIESRNENFPVGTWTLVSSGWTTHTVSDGKGLIKMPLPEGVPRSYAIGSIGMPGVTAYFGLLDICTPKAGETVLVSGAAGAVGNVVGQIAKIKGCRVIGSAGTEEKCEYLKELGFDEVFNYKTTKNLDAKLKELAPEGIDVYFDNVGGEFATTAVLNMKLFGRISCCGAISGYNLKEPEKLSAIYGKMVFSQLKMEGFIVTRYQPRWAEAITALAGWVKEGKIKVREHKTDGFENMFKAFTELFTGANTGKAVLR"
  },
  {
    "reference": "MGGKWSKSNLGGWSAIREKMRRTGPRAEPAADGVGAASRDLERHGAITSSNTAANNAQEEEEVGFPVRPQVPTRPMTYKGALDLSHFLREKGGLEGLIWSQERQDILDLWVYNTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPXDPEKVEEVTEGETNXLLHPXNQHGMEDPEREVLVWKFDSHLAFRHLARELHPEYYKDC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design must be focused on enhancing the efficiency of suppression by virus of host autophagy. The designed protein sequence is ",
    "response#1": "LDCSNIKVDGVMWNFGKLGGAHSISETASSHEGYNTTYTLDICKPLTKTLCKKGAFVCAVRNATDINGIERTMDVIDIAGNFVLNSGRTLDPIFTRLKKEDPKTEGLKMELHGGKHKFGNLLKRQKAVITLLCDRERTGLEGLESPKPDGDKKKDGEKKDDDKKDNKDKEGKSKRDGEENKRSLIFKSYNEEEGTLDLEWKTKYACED",
    "response#2": "LDCSNIKVDGVMWNFGKLGGAHSISETASSHEGYNTTYTLDICKPLTKTLCKKGAFVCAVRNATDINGIERTMDVIDIAGNFVLNSGRTLDPIFTRLKKEDPKTEGLKMELHGGKHKFGNLLKRQKAVITLLCDRERTGLEGLESPKPDGDKKKDGEKKDDDKKDNKDKEGKSKRDGEENKRSLIFKSYNEEEGTLDLEWKTKYACER",
    "response#3": "LDCSNIKVDGVMWNFGKLGGAHSISETASSHEGYNTTYTLDICKPLTKTLCKKGAFVCAVRNATDINGIERTMDVIDIAGNFVLNSGRTLDPIFTRLKKEDPKTEGLKMELHGGKHKFGNLLKRQKAVITLLCDRERTGLEGLESPKPDGDKKKDGEKKDDDKKDNKDKEGKSKRDGEENKRSLIFKSYNEEEGTLDLEWKTKYACEV"
  },
  {
    "reference": "MRRAEENNGFGTIPKRRNQHTPFYASIGMIVVIIVAFTSYHITSYGDDRNRAMRQYSFTFSLAYLAFLVGELLRRCCLFAEEYRHIETRYNGSLKKAIQTTFSFGHNNVLFVASLLFFVVFVASNDPNGSSSVIQGNSTAEPHTEMRQTSGWQGLWGQFIISALLTPLVVHLLGLRELSKVEESQLNEKENKNVADGLAWSYYFGYLKFVLPELEKQIEKTSKFRSKEKFVKKMFILIPSNCFWDDKIPGSDYDPQNRITFEGNTEPLEKTRGGVFLRHYKHSVYEIKDGENEPWFCIMEYATPLLTLYDMSVAQPGELSREERDAQVVVFLRKLQDILEGDRACQGKYELVTFSPDRDLADVMLRKLKDSELEIGG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have 2',3'-cyclic GMP-AMP binding within autophagosome, endoplasmic reticulum membrane to regulate innate immune response, autophagosome assembly, positive regulation of macroautophagy, reticulophagy, activation of innate immune response.2. The design objective is to achieve a protein with helix-helix-turn-helix-helix-strand-strand-strand-turn-strand-strand-strand-helix-turn-helix characteristics.3. The designed protein should have at least Helical; Name=2, Helical; Name=1, Helical; Name=4 transmembrane domains to ensure proper localization.4. The designed protein should contain Extracellular, Cytoplasmic topological domains.5. For general function, the protein need meet that Sensor of cytosolic DNA from bacteria and viruses that promotes autophagy; Exhibits guanine base-specific ligand recognition; Acts by recognizing and binding cyclic GMP-AMP (cGAMP), a messenger produced by CGAS in response to DNA in the cytosol; Following cGAMP- binding, promotes the formation of autophagosomes, leading to target cytosolic DNA for degradation by the lysosome The designed protein sequence is ",
    "response#1": "MAKTPSDHLLSTLEELVPYDFEKFKFKLQNTSVQKEHSRIPRSQIQRARPVKMATLLVTYYGEEYAVQLTLQVLRAINQRLLAEELHRAAIALLKTSPSPERALFLKYARTPTLDPDTMHARLRLSPDGLTVRCSLLGRLGPRPAPRFDALRQVLGRDGFAAGRHYWEVDVQEAGVGWWVGAAYPSLRRRGASAAARLGCNRESWCVKRYDLEYWAFHDGQRSRLRPRRDPHRLGVFLDYEAGILAFYDVAGGMSHLHTFHAAFQEPLYPALRLWEGPISIPRLLWESRYRTLRNVGNESDIKTRKSESEILRLLQPGPSEHSKSFDKWTTKINECVMKKMNKFYEKRGNFYQNTVGDLLKFIRNLGEHIDEEKHKKMKLKIGDPSLYFQKTFPDLVIYVYTKLQNTEYRKHFPQTHSPSEMEMFNVPELIGAQAHAVNVILDAETAYPNLIFSDDLKSVRLGNKWERLPDGPQRFDSCIIVLGSPSFLSGRRYWEVEVGDKTAWILGACKTSISRKGNMTLSPENGYWVVIMMKENEYQASSVPPTRLLIKEPPKRVGIFVDYRVGSISFYNVTARSHIYTFASCSFSGPLQPIFSPGTRDGGKNTSFALLKTSPSPERALFLKYARTPTLDPDTMHARLRLSPDGLTVRCSLLGRLGPRPAPRFDALRQVLGRDGFAAGRHYWEVDVQEAGVGWWVGAAYPSLRRRGASAAARLGCNRESWCVKRYDLEYWAFHDGQRSRLRPRRDPHRLGVFLDYEAGILAFYDVAGGMSHLHTFHAAFQEPLYPALRLWEGPISIPRL",
    "response#2": "LDAQAAQQLQYGGSLGTVGRLSITVVQAKLAKNYGMTRMDPYCRVRLGYAVYETPTAHNGAKNPRWNKVIQCTVPPGVGSFYLEIFDERAFSMDDRIAWTHVTIPEGLREGSVVDEWHSLCRFDALRQVLGRDGFAAGRHYWEVDGPTLDPDTMHARLRLSPDGLTVRCSLLGRLGPRPAPRFDALRQVLGRDGFAAGRHYWEVDVQEAGVGWWVGAAYPSLRRRGASAAARLGCNRESWCVKRYDLEYWAFHDGQRSRLRPRRDPHRLGVFLDYEAGILAFYDVAGGMSHLHTFHAAFQEPLYPALRLWEGPISIPRP",
    "response#3": "MAKTPSDHLLSTLEELVPYDFEKFKFKLQNTSVQKEHSRIPRSQIQRARPVKMATLLVTYYGEEYAVQLTLQVLRAINQRLLAEELHRAAIYGDKLKVSIPDEFDLLIHLVFPENDKIIVKADASKPGNVILDMTKVMEIIGSQEHNKPVFDRLQKIVNNKKQLLEDKLNSFLESIMTQTLNKMGNQIEVAGRISHLQYKKCGPAHTIFVKGSCKYSVDFVPAIRLSAAQVVLAPEQRIHFGETLYWDAIPKPMKPAKTDNTSFTSSFYEAERRLLYYGDKLKVSIPDEFDLLIHLVFPENDKIIVKADASKPGNVILDMTKVMEIIGSQEHNKPVFDRLQKIVNNKKQLLEDKLNSFLESIMTQTLNKMGNQIEVAGRISHLQYKKCGPAHTIFVKGSCKYSVDFVPAIRLSAAQVVLAPEQRIHFGETLYWDAIPKPMKPAKTDNTSFTSSFYEAERRLLYVWNGTINVEVVVSDAIVVPNTTLADKSCHIVMLRDAYLGFYLPTVVRKLADTIKVPYESDYRNWWFEYNGEGVPWEYPCGVLFDLLNKKREKQEVAVKTFCEGSPRAQREVSCLQSSRENSHLVTFYGSESHRGHLFVCVTLCEQTLEACLDVHRGEDVENEEDEFARNVLSSIFKAVQELHLSCGYTHQDLQPQNILIDSKKAAHLADFDKSIKWAGYGDKLKVSIPDEFDLLIHLVFPENDKIIVKADASKPGNVILDMTKVMEIIGSQEHNKPVFDRLQKIVNNKKQLLEDKLNSFLESIMTQTLNKMGNQIEVAGRISHLQYKKCGPAHTIFVKGSCKYSVDFVPAIRLSAAQVVLAPEQRIHFGETLYWDAIPKPMKPAKTDNTSFTSSFYEAERRLLY"
  },
  {
    "reference": "MSLSVVVLAAGKGTRMRSALPKVLHPVAHKPMVQHVIDSARQLDANAIHVIYGHGAEALLERLGGQQLNFVEQAEQLGTGHAVQQVLPYLNDDEHVLILCGDVPLTRVETLQKLVEVGKATDFAILTVDVPDPTGYGRMIRDSKNAIVGIVEHKDASAEQLQITEINTGIMMASGKALKRWLSQLSNDNAQGEYYLTDIVQMAAAENVEIAYAQPQEFAEVQGVNNRQQLAMLERAYQLRQAQQLMVDGATLLDPARVDVRGSVKIANDVVIDVNVIFEGEVELGEGVVIESNCILRNCRIGAHTQIKSHSIVEQADVAERCEVGPYARLRPGSVLAEGAKVGNFVEMKKTRLGKGSKANHLTYLGDAIVGDGVNIGAGTITCNYDGVNKSVTEIDDGAFIGSNSSLVAPVRVGKQATVGAGSTITKNVEDDELGIARGKQRNITGWQRPQKKG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.2. The designed protein should have magnesium ion binding, glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity within cytoplasm to regulate peptidoglycan biosynthetic process, cell wall organization, regulation of cell shape, UDP-N-acetylglucosamine biosynthetic process, cell morphogenesis.3. The protein should be able to bind acetyl-CoA, Mg(2+) ligand in a variety of conditions4. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).5. The designed protein should contain one or more MobA-like NTP transferase domains that are essential for its function. The designed protein sequence is ",
    "response#1": "VVILAAGMGKRMQSALPKVLHPLAGKPLLRHVIDTARSLSPQKLCVIYGHGGAAVPDMVSALAQETGVTIDTALQQPQLGTGHAVMQAVPQLDDGSATLVLYGDVPLTTADTLRRLVEAAGSDKLGILTVEQLTNPTGYGRIVRENGQVVAIVEQKDASEEQRQIREIYSGIMAVPAKTLKRWLGQLRNDNAQGEYYLTDIVAMAVADGVPVVATHAPDAMEVAGVNSPVQLAELERWYQRQQAQALMEQRAQLAACEAVFQARMRRAAMDAGVTLQAPEMVFFSHDTVLEADVFVEPNVVFGPGVHVEGGTRIRAFSHLEGAHVGRDATVGPFARLRPGAELAADTHIGNFVEIKNATVAEGAKVNHLTYIGDADIGAKSNIGAGTITCNYDGYLKHRTTIGAGCFVGSNATLVAPVALGDGAYVAAGSVVTDAVPADALAIGRGRQRNIAGWQRPTKQPKA",
    "response#2": "VIILAAGEGTRMKSATPKVLHRIGGRSLVGHAIAAARVTAPQHLAVVVRHERDLVAAHIAEVDAAATIADQDDVKGTGRATECALDALPADLTGTVLVTYGDVPLLTGETLVDLVARHDESGSAATVITTVLDDPTGYGRILRDDRGVVGIVEERDADTAQLAIDEINSGILAFDGALLGPALARLRPSNAQGELYLTDVPGIARDDGADVAAFTVDDARLVAGCNDRVELAAMRRTLNRRVLDRHMRAGVTILDPDTTWIEDDVTLAQDVTVLPGCFLQGHTTVASGAVVGPYTTLIDAQIDENAVVERSRVQESHIGKAANIGPWTYLRPGNVFGEETKAGAFVEMKKAHIGNGTKVPHLSYIGDADLGEHTNIGGGTITANYDGVHKNHTTIGSGAHVGAGNLFVAPVTVGDGVTTGAGSVVRHDVPSDSMVYSENTGVTLADPNRVDIRGTVVCGSDVFIDVNVVFEGEVKLDDNVRIGPNCVIRQTQIEQDCEILPNTVIDHAHIGPRCTIGPFARVRPETFLGSEVRIGNFVEVKKSAISKGSKVNHLSYVGDSRIGENVNVGAGTITCNYDGANKHRTVIGDGAFIGSGTQLVAPVRIGEGSTIGAGSTISKDTPANALTLARARGVPGWVARKRPGTPAAEAADAALAGAQDQNP",
    "response#3": "VVILAAGMGKRMQSALPKVLHPLAGKPLLRHVIDTARSLSPQKLCVIYGHGGAAVPDMVSALAQETGVTIDTALQQPQLGTGHAVMQAVPQLDDGSATLVLYGDVPLTTADTLRRLVEAAGSDKLGILTVEQARLPDGLGRILRDAAGRVLAIREHKDASEAERAVGLCNAGLMAINGPHLLDLLARVDNDNASGEYYLTDIVEIAHAAGRAAHPLVVEDAAETLGVNSRAQLAACEAVFQARMRRAAMDAGVTLQAPEMVFFSHDTVLEADVFVEPNVVFGPGVHVEGGTRIRAFSHLEGAHVGRDATVGPFARLRPGAELAADTHIGNFVEIKNATVAEGAKVNHLTYIGDADIGAKSNIGAGTITCNYDGYLKHRTTIGAGCFVGSNATLVAPVALGDGAYVAAGSVVTDAVPADALAIGRGRQENKAGWAARFRAKNRAAKAAKKA"
  },
  {
    "reference": "MIASVRGEVLEIALDHVVVEAAGVGYRLNATPSTLATLTRGAEARLYTAMIVREDSMTLYGFADTEARDLFGLLQTVSGVGPRLAMAVLAVLEPEALRKALAESNVAALTRVPGIGKRGAERMVVELRDKVNLVPVQAGPPGSTPAVAATPVREQVVEALTGLGFPLKQAEQALDTVLAEQPAADTSTALRAALSLLGKNR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair, while the RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR); The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex; RuvA specifically binds to HJ cruciform DNA, conferring on it an open structure.2. The designed protein should be optimized for DNA binding, four-way junction helicase activity activity in order to improve DNA recombination. The designed protein sequence is ",
    "response#1": "MIASLRGTVIDKGLDYVTIECAGVGYQCSGTATTIAELPRGEEVFVTTALVVREDSQTLI",
    "response#2": "MITSIQGTLVSATPLQAIVEVAGFGYEVHIPVTTAERLPAAGAAVKLHTLVIYREDSQTLYG",
    "response#3": "MIGWLQGQKVEAWQQGTRQGVVLACAGVGYEVQIAPRHLSEMEHGQNTFILWIHQVQRDDGSSLFG"
  },
  {
    "reference": "MTSFNDGRTVRVVIPARYGSTRLPGKPLVDLDGEPMIVRVHARVRRALPDADIVVAIDDARIAEVLDARGIRFAMTDTGHASGTDRAAEVARVSGWPDTDAVLNVQGDEPLVPTALLQAFAGFCGAAPDLGVATVACPIDEVALLDEPAIVKVVVDHRGRALYFSRAAIPFCRDGRPADSAGSHGLFLRHIGLYGYANATLQALASAAPCELEQLEKLEQLRALWLGMPIDVMRWPDAPPAGIDTPDDVARVVSLLKRQTHDATEPY",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be designed to localize specifically to cytoplasm in order to perform its role in CMP-keto-3-deoxy-D-manno-octulosonic acid biosynthetic process more effectively. The designed protein sequence is ",
    "response#1": "NNKMILIAGPCVIEGEDITLEIAGKLQSILAPYSDRIQWFFKSSYDKANRSSLNSFRGPGLTEGLRILAKVKETFGVGILTDVHTPQDAYAAAEVCNILQVPAFLCRQTDLLVATAETGAIVNLKKGQFLSPWDMEGPINKVLSTGNNKILLTERGCSFGYNNLVSDMRSIPVLSRSGFPVIFDATHSVQLPGALSTESGGLTEFVPTLSRAALAAGAHGLFIETHTNPKIAKSDAASMLSLEEFAALLPTWDQLFEGTC",
    "response#2": "MK",
    "response#3": "VTLSPSVTVGNEAPFTLFGGINVLESRDLALRAAEEYVRVTQKLGIPYVFKGSFDKANRSSIHSYRGPGLEEGMKILQAVKDTFGVPVITDVHEPWQAAPVAEVADVVQLPAFLARQTDLVEALAKTGRAVNIKKPQFMSPAQIQNVVEKFVEAGNEQLILCDRGTCLGYDNLVVDMLGFGVMKKVSGNRPVIFDVTHSLQQRESGAAASGGRRAQVAELARAGMAVGLAGLFLEAHPNPAEAKCDGPSALPLAQLEPFLAQVKAIDDLVE"
  },
  {
    "reference": "MRVAVLGATGAVGQTMLRVLEERGFPAEEIVPLASERSEGRLLEWRGREWRVRRPTPEAFRGCQVALFSAGAARSREWAAVAAAEGAVVVDNSSAWRMDPEVPLVVPEVNGARIHERPRGIIANPNCVVIQLVLALEALRRAAGLRRVWASTYQSVSGAGARGVAALRTELDGGTPETTPFPASIAGNVIPFIGSAAEGGWTEEEEKIRNEARKILDLPGLAVAATCVRVPVEVGHGVAAAVELERPLSVEEAREALAAMPGVALAEAPWGPVPRDVAGQDTVRVGRLRADPDDPRTLHLWIVGDNLRKGAATNAVQIAELVARG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in threonine biosynthetic process, diaminopimelate biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.2. The Acyl-thioester intermediate active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "LNGLFREGLIEWITAMTYQAASGAGAQNMRELLAQMGAVHDSVRGLLADPASAILEIDGKVAQTMRSSAFPTRHFGVPLAGSLIPWIDKDLGNGTSREEWKGDAECNKILGLPAQGEGHIPIESLCVRVGAMRCHSQALTIKLRKDVPLADVEALIAEANDWVKLVPNDRESSVRGLSPAAVTGTMSVPVGRLRKLAMGPEYLSAFTVGDQLLY",
    "response#2": "LNGLFREGLIEWITAMTYQAASGAGAQNMRELLAQMGAVHDSVRGLLADPASAILEIDGKVAQTMRSSAFPTRHFGVPLAGSLIPWIDKDLGNGTSREEWKGDAECNKILGLPAQGEGHIPIESLCVRVGAMRCHSQALTIKLRKDVPLADVEALIAEANDWVKLVPNDRESSVRGLSPAAVTGTMSVPVGRLRKLAMGPEYLSAFTVGDQLLA",
    "response#3": "LNGLFREGLIEWITAMTYQAASGAGAQNMRELLAQMGAVHDSVRGLLADPASAILEIDGKVAQTMRSSAFPTRHFGVPLAGSLIPWIDKDLGNGTSREEWKGDAECNKILGLPAQGEGHIPIESLCVRVGAMRCHSQALTIKLRKDVPLADVEALIAEANDWVKLVPNDRESSVRGLSPAAVTGTMSVPVGRLRKLAMGPEYLSAFTVGDQLLR"
  },
  {
    "reference": "MSQYSGKICFYEGRCFTGRCLEVYGDCDNFQDRGFMNRVNSIRVESGAWICYDHPDFKGQQYILERGEYPEFQRWNSHNDHMGSCRPIRMHGEQYRMELFEGCNYTGQCMELCDDCPFLQSRGFNTNCVNSVRVFGDGAWVMYEEPNFRGRMYIVERGNYCGHNEWQAQNPHIQSIRRIVNYF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in lens development in camera-type eye, potentially with improved properties such as stability, specificity or selectivity.2. The protein contains novel Beta/gamma crystallin 'Greek key' domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "KIVLYENPNFTGKKIEIIDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGYQYLFEKGDYKDSSDFGAQHPQIQSVRRR",
    "response#2": "KMRISERGDFQGQNMEFFEDCPNTYDRFRFRDIHSCNVFDGNWMFYEEPNYRGRQYYLRPGEYRRYSDWGASSARIGSFRRC",
    "response#3": "KMRISERGDFQGQNMEFFEDCPNTYDRFRFRDIHSCNVFDGNWMFYEEPNYRGRQYYLRPGEYRRYSDWGASSARIGSFRRR"
  },
  {
    "reference": "MKFSEKWLRSWANPQVSHDELVARLSMVGLEVDADLPVAGAFSGVVVGEVLSTEQHPDADKLRVCQVSNGSETFQVVCGAPNVRAGLKIPFAMIGAELPDDFKIKKAKLRGVESFGMLCSAKELQISEENAGLLELPADAPVGQDVRTYLELADYTIEVGLTPNRGDCLSLAGLAREVSAIYDVPLAPVAVDAVAAQHDETRPVELAAPAACPRYLGRVIRNVDLSRPTPLWMVERLRRSDIRSIDPVVDVTNYVMIELGQPMHAFDLAEINGGVRVRMAEDGEKLVLLDGQEITLRADTLVIADHQRALAIAGVMGGEHSGVSDSTRDLFLEAAFFDTIALAGKARSYGLHTDSSHRFERGVDSQLARKAMERATRLILDIVGGEPGPIVEQVSEAHLPKVAPITLRAERVTQMLGMPLDAAEIVRLLQALELTVVADGEGQWSVGVPSHRFDISLEVDLIEELARLYGYNRLPVRYPQARLAPNNKPEARAALPLLRRLLVARGYQEAITFSFIDPALFELFDPGTQPLTLANPISADMAAMRSSLWPGLVKALQHNLNRQQSRVRLFESGLRFVGQLEGLKQEAMLAGAICGKRLPEGWANGRDGVDFFDAKADVEAVLASAGALGDFSFVPGEHPALHPGQTARIEREGRLVGYLGALHPELAKKLDLEQPVFLFELLLAEVVDGHLPKFRELSRFPEVRRDLALLVDQDVPAQDILTQIRAAAGEWLTDLRLFDVYHGKGIDPHRKSLAVGLTWQHPSRTLND",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a high affinity for Mg(2+).2. The engineered protein is expected to adopt a strand-helix-helix-strand-strand-strand-strand-strand-turn-strand-strand-strand-helix-helix-strand-based conformation.3. The protein should be designed to catalyze the following reaction ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) +   L-phenylalanyl-tRNA(Phe).4. A protein that can perform tRNA binding, magnesium ion binding, phenylalanine-tRNA ligase activity, ATP binding in phenylalanine-tRNA ligase complex to support phenylalanyl-tRNA aminoacylation.5. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.6. The protein contains novel FDX-ACB, tRNA-binding, B5 domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "IESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWFDTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNISFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPEVYSGFAFGMGMERLTMLAAPYIPVTMPAGRMLCHLPPQHLREVKRLRDVMVAAGFNETVNYSFVAPEAWDRLELSDDDPRREPVKILNPLTEDQSVMRTSLVPSLLECVTRNVAYQNSDLHLFELRPVFLTHTDDVLPIEKWRLCAVLCGRREQEGWRQNDEKVDFYDLKGVVEQILDSFNVAKVTWDGSSSENFLHPGKSCTLKQKKTVLGTLGEVHPKVLAAFDIDQPVYLLDLDCAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLKVAVATVGAVLPGDFKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPLGTDIETSLPPSFTVGKPHPVQKLYSLVRNIMVGLSYIENTPFTLTSGDVSYSLMHRPENPAALHVLHPISEDQTIIRTDILPLLMESLSINRSRELPQKIFACGDVVENLVTYPKMAAASIHTSADFSEIYAVMDAFCRMMSIEYEVRDSADDAFIPGRRGDIYVSGEKIGVFGEINPDVLVGFGLEHQAVAFEIDAETSEIRPYLGSAVIRNVHMDNAMIESLMGLQESLHWAVGRGRKKVAIGVHDLDKIEGPFSYIAADRKTTFVPLDYDVEMTMEEILAEHPKGKAYARIVEEFERFPLITDAKGRVCSFPPIINGELTRVTEDTQNILLDVTGIEPRAVSVAVKILCAAFVEMG",
    "response#2": "MPKVNINRDILYKALGFYIKTYTQEQFEDLCFAFGVELDEVTSEREMKKNETGVEDLTVSDDVIYKIDVSANRYDLLCLEGIARALNVYNHSFTSKEIPNCNTIGRVQPINKLSELLANEIALAGFTEIMTFVLCQNRDNFTALNKADDGSSVKISNAVSEEFTEVRTNLVSTLLKSVSANKAAPLPLKMFEISDVSIKGSLGNKDLSDPNSNNSDVGAYNKRMLGAIYCNQSAKIEVIHGLLDRIMLVLDIKLDATRSSNKGYYLELSNDKLFLPGTGINVIVNGKRVGHMGIVHPLVLKNYSCSFPCTILELEKEIPNCNTIGRVQPINKLSELLANEIALAGFTEIMTFVLCQNRDNFTALNKADDGSSVKISNAVSEEFTEVRTNLVSTLLKSVSANKAAPLPLKMFEISDVSIKGSLGNKDLSDPNSNNSDVGAYNKRMLGAIYCNQSAKIEVIHGLLDRIMLVLDIKLDATRSSNKGYYLELSNDKLFLPGTGINVIVNGKRVGHMGIVHPLVLKNYSCSFPCTILELENC",
    "response#3": "IESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWFDTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNISFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPEVYSGFAFGMGMERLTMSAADYVQLAERKVTLAVQRVNEILGLKLGMDDIQAHLLSIGLEVQPVEEKDIDRLVVTIPSFRPDLERDIDLVEEVARLNGYDNIPDTHRFFENLKHKALACGFKEVIHYVFYSKEKQQKLGFEVLEDPLELQNPITTELNTLRTSLVCGLLDASLRNKNLGFKSIALYEKGSVYNSKREEIQKLGFLISGLQKKESYPDTKGKAWDFYSFAECVSKVIGDFSLEKLTTQTPINHPYQSAKIIQNHEIIGVIAKIHPKVIQELDLFESYYAEIDLTKPGKKKNLGSRHPISIIKHRIINIFNKIGFKICEGPEIEYDWYNFTALNFPYYHPAREMQDTFFIKKSNKLLRTHTSSVQIRYMEKNKPPIRIISPGRVYRNEKISAYSHCMFHQIEGLYLDKKVSFKDLKSTINFFIKNFFGKSITRFRPSYFPFTEPSAELDILWNNKWLEIIGCGMVDPIVLKNVNIDPDLYSGFAFGMGIERITLMIYKINDIRYFFENDLRFLKQF"
  },
  {
    "reference": "MDAKGLAAVVLAAGKGTRMKSALPKVLHELNGQPMVHYPVQQAARLGCQPTVLVVGHGAEEVKECLAEQPVDFAVQEQQLGTGHALLSARGALVDFSGTLLLLCGDVPLLRQATLQQLLDYHRSEGAAATVLTATMTDPFGYGRILRDGSEVLGIVEEKDATDEQRTICEINTGIYVFEAPLVFEILSGLGCDNAQGEYYLTDVLATLRADGRKVRALVMDDPAEAMGINSRVQLAEAATIMRQRINERLMLDGVTLIDPAATYVDEQVEVGADTVIHPGVCLWGATRIGQGCVIEPQVTVENCQVGDQVRLKTGSVLEGSQVGAHSDVGPMAHLRPGTVLAGHNKIGNFVETKKAFIGEGSKASHLTYIGDAELGARVNIGCGTITCNYDGVNKHKTIIEDDVFVGSDTQFVAPVRIGRNSLVGAGSTITKDVPADSLALSRSPQKVIEGWVLRKQKKKK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that can perform magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity in cytoplasm to support cell morphogenesis, UDP-N-acetylglucosamine biosynthetic process, regulation of cell shape.2. We require a protein that can specifically bind to the metabolites produced in the Nucleotide-sugar biosynthesis pathway.3. The acetyl-CoA binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "VVILAAGQGKRMRSDLPKVLHRLGGRPLLAHVLDTARELGAGRICVVYGHGGEAVREALDADDLVWVRQEPQLGTGHAVLQTLTFLDADAPTLVLYGDVPLIGAATLSRLVGLEVPDPGRYGRLVTEGDVVERIVEAKDATPEQLSIRECNTSVYVFDAAVLRSALGRLGNDNAQGEYYLTDAIEAARADGPGVSIVDPANTYLDMDVTIGRDTLVEPGCVITGATVIGEGCHIKPHCTIEDSRLGDETVIGPSAHLRPGTELGRGCRIGNFVEVKNSKLADGVKADHLAYVGDADVGEGSSFGCGAITVNYDWDQKHRTTVGRDVKVGCNANLVAPVTLADGAVVAAGSTITTSVPEQSLAVARAKGVSIADSHRIDVRGELRCAADVFIDVNTVFSGTVIIGSGSQIGPNCVLNNVTLGENCEILANSVLDNCVIGNDCHIGPFARLRPGTKLADKCKIGNFVETKNAIFATASKASHLSYLGDVNIGKDVNIGAGTITCNYDGVNKYETIIEEGAFIGSDTQLVAPVTIGAYATIGAGSTIRKDVPAGELTLTENRQRNVEGWVEANRPGTAAAEAAEAAR",
    "response#2": "VVILAAGQGTRMKSRRSKVLHEIAGRPMVGFPVALARELSASEPVVVVGRDAEDVQAALAGQAKFVLQAEQNGTGHAVLCAQDALADARDAILILYGDTPLLRLESLRRMAEMLAADDADLVMLTSPELGYGRIVRDDDGSVERIVEQKDASPEELAIREINSSIYVFRAGVLKEHLGRIGNDNAQGEYYLTDIVGLAVADGQTVGAHQITDSWQVEGVNDRVQLSALGAEYNRRIVERWQRADVTIGRDTLVEPGCVITGATVIGEGCHIKPHCTIRDSGVQVGQGALIGPFARLRPGAQLGREVHIGNFVEVKDLAEAEAAFQARRRQEMMESGVTLIAPDTVFFSHDTRIAADVVVEPNVVFGPGVNVASNVRIKAFSHLEGASLGEGAEAGPFARLRPGAELGANAKVGNFVEVKKARLGEGAKASHLTYLGDTDVGAHANIGAGTITCNYDGFFKHRTVIGEGAFIGSNTSLVAPVTIGAGAMTGSGSVITEDVPDNALALSRARTVIGDGA",
    "response#3": "VILAAGKGTRMKSALPKVLHKVAGRSMLGHVLALASTVPGATIAVVVGPGMDEVRAEVSRLAPAARVFVQENQSGTADAVLAARPALDGHSGDVIVLYADTPLIEAGTLSALGDALDAGSGVAVLGFEADDPGAYGRLVMDGDTLLKIVEFKDATEAERAITLCNSGLMAVRSADLFALLARVGNDNAAGEYYLPDIVMLAAAEGRACAVIETAAAEVAGVNSRGELAEVEAQFQRNRREAXDLAEAEAAFQARRRQEMMESGVTLIAPDTVFFSHDTRIAADVVVEPNVVFGPGVNVASNVRIKAFSHLEGASLGEGAEAGPFARLRPGAELGANAKVGNFVEVKKARLGEGAKASHLTYLGDTDVGAHANIGAGTITCNYDGFFKHRTVIGEGAFIGSNTSLVAPVTIGAGAMTGSGSVITEDVPDNALALSRARQEAKPGWAAKFRALMQGKKGK"
  },
  {
    "reference": "MKHVVMGVTGSIAAYKACDIISALRKSGVDVHVILTHAGAEIITPLTLETISGNRVVKDMFDSDRHFEVEHISLAKLADLFLVAPATANFIGKMASGIADDMLTTTVMAITAPVMIAPAMNTNMYLDAATQANIETLKRRGCEFIAPATGHLACGDEGIGKLAAVEDIVRAVLDKLNVKRDLEGKRVLVTAGPTREAIDPVRYITNRSSGKMGYAIAEAAAERGADVTLISGPVSLRTPGGVKRVDIVSSDELCDSVIERFPGCDILVMAAAPADFTPAEFSSEKIKKGGDNLTLNLRATRDILKTIAPLKSNQKVMGFAAETHNVENNAIEKLKRKKLDFIAANDVTAEGAGFGTDTNIITLYGADGSREHSGMVTKREAADWLLDRLVK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design must be able to perform metal ion binding in order to contribute to pantothenate catabolic process.2. The protein must bind to Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "SHKYERGHTLVASGGATRTGAARLAARAALRIGSGLVTVASPPEALGVNAAQLTAVMLRGCDGPEGLHAILQDPRFNALVLGPALGVHAATRAMVSVAVHARRSLVLDADALTSFEGLASELHGAFRHAPTVLTPHGGEFNRLFKGHPDILDPESKIERTRRAAAYTGAVVLLKGADTVIAAPDGRAAINENGTPYLATAGSGDTLCGLIAGLMAQGLPAFEAACAGAWIHAEAGAQFGPGLISEDLAEQVPAVLR",
    "response#2": "SHKYERGHTLVASGGATRTGAARLAARAALRIGSGLVTVASPPEALGVNAAQLTAVMLRGCDGPEGLHAILQDPRFNALVLGPALGVHAATRAMVSVAVHARRSLVLDADALTSFEGLASELHGAFRHAPTVLTPHGGEFNRLFKGHPDILDPESKIERTRRAAAYTGAVVLLKGADTVIAAPDGRAAINENGTPYLATAGSGDTLCGLIAGLMAQGLPAFEAACAGAWIHAEAGAQFGPGLISEDLAEQVPAVLA",
    "response#3": "SHKYERGHTLVASGGATRTGAARLAARAALRIGSGLVTVASPPEALGVNAAQLTAVMLRGCDGPEGLHAILQDPRFNALVLGPALGVHAATRAMVSVAVHARRSLVLDADALTSFEGLASELHGAFRHAPTVLTPHGGEFNRLFKGHPDILDPESKIERTRRAAAYTGAVVLLKGADTVIAAPDGRAAINENGTPYLATAGSGDTLCGLIAGLMAQGLPAFEAACAGAWIHAEAGAQFGPGLISEDLAEQVPAVLAE"
  },
  {
    "reference": "MSKQKANIAILGATGVVGREITKIVDELKIDFNSIKFLSSAKSAGTKIEFQGKTYTVEEAKPKSFDGVNIVLASAGGSTSQKFAPEIVKRGGVLIDNSSAFRMDKDVPLVIAYVNDEDLKKHKGIIANPNCSTSQLMLVLRPLNDKFKIKRLLVSTYQAVSGAGLKAINELTENTKATLEGKDFKNEAFKKPISFNVIPQIDVFCENAYTKEEMKVVNETKKILHLPQDLPISCTAARVPVYNGHSEAVDIEFEEKVTPEEVREILSNSFGLKVIDNPENFEYPTTRDASGVDPVFVGRIRKNLAFDNGISLWCVADNLRIGAALNTVRICKKVIEMGLY",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be able to perform NADP binding, protein dimerization activity under specific conditions.2. The designed protein must have a Acyl-thioester intermediate active site that is highly conserved among related enzymes.3. The protein should have a high affinity for substrate. The designed protein sequence is ",
    "response#1": "LGGLFAEDLVEWVSPMTYQAASGSGAKHMRELITQMGEINEAVANQLDNPASAILEIDKLVAEKMRSDEFSTDQFGVPLAGSLIPYIDTQLPSGQSREEWKAGAETNKILGTQDQAIPIDGLCVRVGAMRCHSQALTIKLKKDLPVEEIEAKLAAANDWVKVIPNDREASINELSPTVATGTLSIPVGRIRKLEMGPEYISAFTVGDQLLY",
    "response#2": "LGGLFAEDLVEWVSPMTYQAASGSGAKHMRELITQMGEINEAVANQLDNPASAILEIDKLVAEKMRSDEFSTDQFGVPLAGSLIPYIDTQLPSGQSREEWKAGAETNKILGTQDQAIPIDGLCVRVGAMRCHSQALTIKLKKDLPVEEIEAKLAAANDWVKVIPNDREASINELSPTVATGTLSIPVGRIRKLEMGPEYISAFTVGDQLLY",
    "response#3": "LGGLFAEDLVEWVSPMTYQAASGSGAKHMRELITQMGEINEAVANQLDNPASAILEIDKLVAEKMRSDEFSTDQFGVPLAGSLIPYIDTQLPSGQSREEWKAGAETNKILGTQDQAIPIDGLCVRVGAMRCHSQALTIKLKKDLPVEEIEAKLAAANDWVKVIPNDREASINELSPTVATGTLSIPVGRIRKLEMGPEYISAFTVGDQLLF"
  },
  {
    "reference": "MNLPVLSSAQMRTAEEAAFARGVEVEALMDQAGNGVAQAVTRFFRKPGKCIVFTGKGHNAGDALVAAERLRRLGWKIEVRLAFQEADCSGLMRKKLESLRRRPPEILGATPSRGGGTDVGVIIVELLEEAAEQLSAAQEAMAAEAYLGTAGPLIILDGLLGLGAKPPLRDPIRAACRCINYLRANKGAYVFAVDLPTGLDGDSGKTDRDCVIADFTVTIGFAKPGLLADDALNFVGRLEVVQLDELRAPEKKAKEVVAALSAFRELLPRRKFSTYKNQCGRIGVVAGSRGFIGAALMTSQAALRAGAGLVEVFVPEEIYEIVAGAAPMESMVKPLKSYRDLLKEKADVWAVGPGLGKSHAGEILELIEKAKQPMVIDADGLNILAEKISTLKRCKGKRLLTPHPGEMKRLSQREKETRTKTAIKFCHRFPVTLLLKGSRTIVAEQYRPLSYNTTGNPGMATGGMGDILTGVCAGLAGQGLSLYDAACLGAWLCGRAAEIAIFDGSQSEQSLLPRDVLDQLGNAFNEL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must be optimized for ATP binding, NADPHX epimerase activity.2. The protein have enhanced (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.3. The (6S)-NADPHX, AMP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "DKYTQGVTGVLAGSAAYPGAAILCAGAAVAATSGMVRYAGSAAAEVVDHWPEVVIAPSAAAAGRVQAWAVGPGLGTDETAAAALWFALDTDLPVIVDADALTMLAAHPDLVAGRTAPTVLTPHAGEFARLAGEPPGADRVGAARALADRFGATVLLKGHVTVIAEPGGGSVYLNPAGQSWAATAGSGDVLSGMIGALLAAGLPAGEAAAVAVFVHTRAAALAAADPGAGTAPASAL",
    "response#2": "FTPEAMRAADARAVELGYPSLLLMEAAGRAVAEAALASWPEARSYAVVAGKGNNGGDGLVAARWLRLWGREVRVFAPEGHRGDAALARAALEAHLEIEPLEDLDLHGAEVVVDALFGTGLARALEGVWAEYVARINASAARVVAVDVPSGLPHAPHVEADLTVALAGLKPVHVFYPARSACGRIQLAPIGMP",
    "response#3": "RTMHKATYGKVAIIAGSPDYTGAPVFAAEAALTTGTGTIHLIVPQSIADVVRVKVDGPIVQAFAGDVLTVEALPHILELLEDTTAALIGPGLGRDPRTLEAVLLLATAFKGTLILDGDALFACVGHLDVLWKRDAPTILTPHSGEFARLVGLTPEDVEKGGTLSSAQTLAAQASAVVHLKGHRSLTVLPDGSLYINTTGNPGMAKPGMGDALAGIILSLVARGVAPDLATAQGAFIHGLAGDAAAEVMGEESMGA"
  },
  {
    "reference": "MQFENTKAFAQTQDANDPLKGLKNRFHHPNLNGKPATYFCGNSLGLAPKTAVENVNQELDDWAEFAVEGHFHAKTPWVSYQDIFPEQLSKLVGALPKEVTVMNGLTVNLHLLMVSFYQPTKERYKIICEAKAFPSDQYALETQVKHHGFKPEDAIIEVAPREGEDLIREEDILKAIAEAGDELALVMIGGVNYYTGQVFDMKTITEAGHKVGAQVGFDLAHGAGNIILELHDWDVDFACWCSYKYLNSGPGNVSGMFVHERHCNNPELNRFAGWWGNDPDSRFKMEPGXIHAEVGMEKLREKSLKLTGYLEFLLDDLNSKDVRIITPRNPEQRGCQISIYVDNEAKKLHQALLEECVITDYREPNVIRLAPVPLYNSFEDVWTFVDILRKHLNA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have kynureninase activity, 3-hydroxykynureninase activity, pyridoxal phosphate binding to facilitate 'de novo' NAD biosynthetic process from tryptophan, tryptophan catabolic process, anthranilate metabolic process, quinolinate biosynthetic process, L-kynurenine catabolic process.2. The protein should be able to bind pyridoxal 5'-phosphate ligand in a variety of conditions3. A protein should be able to manipulate the activity of enzymes in the quinolinate from L-kynurenine: step 2/3 pathway, either by activating or inhibiting their function.4. The protein should have a fast turnover rate for H2O + L-kynurenine = anthranilate + H(+) + L-alanine. The designed protein sequence is ",
    "response#1": "MDHLRTEDIVSKLAEIGDEVAVVWIGCVQYYTGQFFEVEPIARKTHEIGAYFGLDLAHAIGNVPVKLNDWGVDFAAWCTYKYLCGGPGSVAGYYIKDGLDDGGRRLAGWWGHNRQTRFHMSPEFDPIPGAGGYQHSNPPVFSSLPLIATLEIIDKAGGIDVLREKSERLTGYFWDLLTSSKWYRGIASPDRQERGHRARKRFGQNFLLDQRCLGTITSAIDPQPCDHVIEIGPGLGALTGPLLARGCTVTAIEKDRGLAALLRQRFAGEPRLLLHEADALAAPFAGLPQAFRVCSNLPYNIATPVLFKLLEAWPRIRDMHFMLQREVAERLAAAPGDRAYGVPAVRLAQLSRVTHLFRVPPGAFNPAPKVDSAVVRLTPRAEPRVDSELFLQLVRLSFGQRRKMLRNTLGGLLPPGCAGELARAGIDPTRRGETLSVDEWVALARAALVGAKQNEVAVMGSLTSNLNALLIHFYKPKGKRTKILFEKQAFPSDYYAFLNIVQVFGYDASHLIQIEIPKGETYIKTETILDVFDKYEDEIAIVCLPGIQYYTGQFFDIAKITKHVKTSAPDVVVGWDLAHAVGNVPLSLHDWGVDFAAWCSYKYLNAGPGAIAGIFVNEKYTEQNKPENA",
    "response#2": "LVGAKQNEVAVMGSLTSNLNALLIHFYKPKGKRTKILFEKQAFPSDYYAFLNIVQVFGYDASHLIQIEIPKGETYIKTETILDVFDKYEDEIAIVCLPGIQYYTGQFFDIAKITKHVKTSAPDVVVGWDLAHAVGNVPLSLHDWGVDFAAWCSYKYLNAGPGAIAGIFVNEKYTEQNKPENE",
    "response#3": "DHLRTEDIVSKLAEIGDEVAVVWIGCVQYYTGQFFEVEPIARKTHEIGAYFGLDLAHAIGNVPVKLNDWGVDFAAWCTYKYLCGGPGSVAGYYIKDGLDDGGRRLAGWWGHNRQTRFHMSPEFDPIPGAGGYQHSNPPVFSSLPLIATLEIIDKAGGIDVLREKSERLTGYFWDLLTSSKWYRGIASPDRQERRHAAALEALGDHLRTEDIVSKLAEIGDEVAVVWIGCVQYYTGQFFEVEPIARKTHEIGAYFGLDLAHAIGNVPVKLNDWGVDFAAWCTYKYLCGGPGSVAGYYIKDGLDDGGRRLAGWWGHNRQTRFHMSPEFDPIPGAGGYQHSNPPVFSSLPLIATLEIIDKAGGIDVLREKSERLTGYFWDLLTSSKWYRGIASPDRQERRHAAALEALGALGYRTVDPLSTGARRS"
  },
  {
    "reference": "MLRERAAHILAAIQNRRAVVLGDLLYDSFVYGDTHRISREAPVPILSEKRRTAMLGGAGNLARNLSSLGGEVRLISVAGTDADGEAAVELVKAACGDADGVVRTSGRPTPSKIRYVSNNQQMFCVDRDPGAAIDAETEQVLLARIKTALQDSDILIVSDYGRGVITRDLVQAAIALAREAGKPVSVDPRGSDYSRYDGASIIKPNAGELEAETGLPVRSDETAADALMALKTSLARTDAVMVTRGGAGMTLLDENGAVHHHRSRPREVYDVSGAGDTALAGLSLAMAAGAALKDAMAFADLAAGVAVGKPGTATVSPEEVLDAAEAGQEAPDWRIMSRPAMAKLSHEWRQNGLKVGFTNGCFDLLHPGHLSVLRHAASVCDRLIVGLNADASVKRLKGENRPINDEMTRATMLASLEMVDRVVIFQEDTPAALIEEVAPHVMIKGADYVAEELPGADFMRRSGGEIVLAPLMDGLSTTNIVNKLRNER",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein contains novel Cytidyltransferase-like domains that confer a unique function or activity.2. The protein must exhibit heptose 7-phosphate kinase activity. The designed protein sequence is ",
    "response#1": "MTNGCFDILHAGHIAYLEEAKALGDRLIIAINNDESVSRLKGKKRPINSLEERMKVLNGLISTDWIVPFEEDTPINIIENIKPDILVKGGDYNLSEVVGAKIVNEYGGEVKILSYIEGFSSSNIF",
    "response#2": "MTNGCFDILHAGHVSYLENARKLGDRLIVAVNSDESVKRLKGESRPVNPLSQRMAVLAGLGAVDWVVPFEEDTPQRLIASLLPDLLVKGGDYQVHEIAGGAEVIAAGGQVKVLNFEDGCSTSNIIFG",
    "response#3": "MTNGCFDILHAGHVSYLENARKLGDRLIVAVNSDESVKRLKGESRPVNPLSQRMAVLAGLGAVDWVVPFEEDTPQRLIASLLPDLLVKGGDYQVHEIAGGAEVIAAGGQVKVLNFEDGCSTSNIIS"
  },
  {
    "reference": "MSELASAPKVALGSIAFAVFWMMAVFPSVPFLPIGRTAGSLLSAVLMVIFHVISPDDAYASIDLPILGLLFATMVVGSYLRNAGMFKHLGRLLAWKSQGGRDLMCRVCVVTALASALFTNDTCCVVLTEFVLELAAERNLPAKPFLLALASSANIGSAATPIGNPQNLVIAFNSKITFPKFLMGILPAMLVGMAVNMVMLLCMYWRELGGGAELSVDGKQMEAVEEGRSPASAKSTPQLNGNGNTMMSLEMSENITTKHPWFMQCTEARRKLFLKSFAYVVTVGMVVAYMVGLNMSWTAITTALALVVVDFRDAEPCLDTVSYSLLVFFSGMFITVSGFNKTGLPGAIWDFMAPYSKVNSVGGISVLSVIILLLSNLASNVPTVLLMGDEVAKAAALISPAAVTTSWLLLAWVSTVAGNLSLLGSAANLIVCEQARRAPRNAYDLTFWQHIVFGVPSTLIVTAVGIPLIGKI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must be able to regulate transmembrane transport.2. The protein must exhibit the following characteristics: Silicon efflux transporter involved in silicon transport from the root cells to the apoplast; In the nodes, involved with LSI3 and NIP2-2/LSI6 in silicon intervascular transfer, which is required for the preferential distribution of silicon, such as hyperaccumulation of silicon in the husk; Is coupled with the silicon influx transporter NIP2-1/LSI1 in both exodermal and endodermal root cells for an efficient silicon transport across the cells into the stele. The designed protein sequence is ",
    "response#1": "VFWMMAVFPSVPFLPIGRTAGSLLSAVLMVIFHVISPDDAYASIDLPILGLLFATMVVGSYLRNAGMFKHLGRLLAWKSQGGRDLMCRVCVVTALASALFTNDTCCVVLTEFVLELAAERNLPAKPFLLALASSANIGSAATPIGNPQNLVIAFNSKITFPKFLMGILPAMLVGMAVNMVMLLCMYWRELGGGAELSVDGKQMEAVEEGRSPASAKSTPQLNGNGNTMMSLEMSENITTKHPWFMQCTEARRKLFLKSFAYVVTVGMVVAYMVGLNMSWTAITTALALVVVDFRDAEPCLDTVSYSLLVFFSGMFITVSGFNKTGLPGAIWDFMAPYSKVNSVGGISVLSVIILLLSNLASNVPTVLLMGDEVAKAAALISPAAVTTSWLLLAWVSTVISIIY",
    "response#2": "VFWMMAVFPSVPFLPIGRTAGSLLSAVLMVIFHVISPDDAYASIDLPILGLLFATMVVGSYLRNAGMFKHLGRLLAWKSQGGRDLMCRVCVVTALASALFTNDTCCVVLTEFVLELAAERNLPAKPFLLALASSANIGSAATPIGNPQNLVIAFNSKITFPKFLMGILPAMLVGMAVNMVMLLCMYWRELGGGAELSVDGKQMEAVEEGRSPASAKSTPQLNGNGNTMMSLEMSENITTKHPWFMQCTEARRKLFLKSFAYVVTVGMVVAYMVGLNMSWTAITTALALVVVDFRDAEPCLDTVSYSLLVFFSGMFITVSGFNKTGLPGAIWDFMAPYSKVNSVGGISVLSVIILLLSNLASNVPTVLLMGDEVAKAAALISPAAVTTSWLLLAWVSTVLFSNYFFN",
    "response#3": "SVPFLPIGRTAGALLGAVLMIVFHVISADDAYASIDLPILGLLFATMVVGGYLKNAGMFRHLGRLLAWRSQGGRDLMCRVCVVTALASALFTNDTCCVVLTEFVLELAAERNLPAKPFLLALATSANIGSSATPIGNPQNLVIAFNSKISFISFLLGILPAMLAGMGINMLMLLCMYWKELDGGACSPDEVAAGKQMEAIEEGRRTALNNNKKDDGDAATPASPEDDDGGDAESMMSENISTKHRWFMQCSEHRRKLFLKSFAYVVTVGMLVAYMLGLNMSWTAITTAIALVVVDFRDAEPCLDKVSYSLLVFFSGMFVTVSGFNKTGLPGAIWNVMAPYSKINHVTGVTVLSVIILLLSNLASNVPTVLLMGDEVAAAAATISPAAVTRSWLLLAWVSTVLFSNYFFN"
  },
  {
    "reference": "MSNNYQNTKLKVFTLNSNPSLAEEIASNMGLELGQCSVQTFSDGEIQINIEESVRGCEVFVIQSTCEPVNQNIMELLIMVDALKRASVETINVVIPYYGYARQDRKARSREPITAKLIADLIETAGANRVITLDLHATQIQGFFNIPVDQLVGIPILSDYIESKNLDDIVVVAPDHGSVKRARQLAERLKAPIAIIDKRNPTDTFAGEINVVGHIDGMTAILIDDIIDTGTRVTSGTKALIENGAKEVYACSTHPVLSGMAIDKLQNSQLKELIVTNSIPLSEDKKIDKITELTVAPLISDAIIRVYEQQSVSVLFD",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.2. The active site of the protein must be engineered to enable effective catalysis of ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-   diphosphate + AMP + H(+).3. The designed protein should contain one or more Ribose-phosphate pyrophosphokinase N-terminal domains that are essential for its function.4. A protein with magnesium ion binding, kinase activity, ATP binding is needed to enhance nucleotide biosynthetic process, 5-phosphoribose 1-diphosphate biosynthetic process in a specific context.5. A protein that is capable of catalyzing reactions within the 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1, Metabolic intermediate biosynthesis, 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MKILSGNSNRPLTEAIAAYLGLPLTKCQVRRFADMEIFVEIQENVRGQDTFIVQSTSYPTNDHLMEMLIMTDALRRASARRITAVLPYFGYARQDRKPGPRTPISAKLVANLITRAAAPLLASYFQEHNFDSENLVVVSPDHASVSRARNMAELLGTPIAIIDNRNEESAENLNEIPTEIIGDVKGKVALVIDDMIDTGTRLTISAEALHNAGATEIYGAATHAIFSRNAPQILQASKLSKVIVTDPLRAATPVLIEDIKRQRFENYVVVSPDVGGVVRARAIAKRLDDADLAIIDKRRPKANVSQVMHIIGDVRGKTCVLVDDIVDTSGTLCKAANALKEHGAEHVVAYITHPVLSGPAIENIEASSLDELVVCDTVQTPANLPASVKLITLGDLLGNALQRIHEGGSLYDVMG",
    "response#2": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGADRALTVDLHAPQIQGFFNVPVDHLMVFAGNAHPELAKKVVQKLHIPMGDATVGKFSDGETSIEINENVRGRDVFVIQPTCNPTNDNLMELIVLVDALRRASATRITAVIPYFGYARQDRRVRSMRVPISAKVVADMMTKVGADRILTMDLHAPQIQGFFDVPVDHVYGATPVLIEDIKRQRFENYVVVSPDVGGVVRARAIAKRLDDADLAIIDKRRPKANVSQVMHIIGDVRGKTCVLVDDIVDTSGTLCKAANALKEHGAEHVVAYITHPVLSGPAIENIEASSLDELVVCDSIPVPEDRQFSKLTVLSVAPLLGEAIRRIHTGQSVGALFVDN",
    "response#3": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGANRAISLDLHAPQIQGFFNVPVDNLYAPVLVRDIQARLKLDNVMVVSPDVGGVVRARALAKRIDAPLAIVDKRRERAGESEVMNIIGDVDGKICILIDDIVDSGGTLCNAADALRAQGASDVYAYITHGVLSGGAAAAAPLLASYFQEHNFDSENLVVVSPDHASVSRARNMAELLGTPIAIIDNRNEESAENLNEIPTEIIGDVKGKVALVIDDMIDTGTRLTISAEALHNAGATEIYGAATHAIFSRNAPQILQASKLSKVIVTDTIPLSDEQQFAGRIRQVSIAPLLAQAIVRIHEQRSVSELFV"
  },
  {
    "reference": "MGGKWSKSSIVGWPXVRERLRQAPAPAAPGVGPASQDLDKHGALTSXNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTXKGAXDLSHFLKEKGGLDGLIYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVDXDEVEXATXGEDNCLLHPICQHGMDDEEXEVLXWXFDSXLALXHRARELHPXFYKDC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis.2. The designed protein should contain at least one Dileucine internalization motif; necessary for CD4 internalization, Diacidic; necessary for CD4 internalization, PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif to achieve its intended function.3. A protein that contributes to suppression by virus of host autophagy by functioning in host cell Golgi membrane, host cell plasma membrane, virion component with SH3 domain binding, GTP binding. The designed protein sequence is ",
    "response#1": "MGGKWSKNSMVDWPTVRERMKRAEPAAEGVGAASRDLEKHGAITSSNTAETNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGFPAGTRWPLTFGWCFKLVPVEPEKVEEATEGENNSLLHPMSLHGMDDPEKEVLQWKFDSRLAFHHMARELHPEYYKDC",
    "response#2": "MGGKWSKPRKGWPAIRERMRRAEPAAEGVGAASRDLERHGALTSSNTPTTNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQKRQDILDLWIYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPDKVEEANEGENNCLLHPMSQHGMEDEEREVLMWKFDSRLAFHHMARELHPEYYKDC",
    "response#3": "MGGKWSKSSIVGWPAVRERMKRTEPAAEGVGAASQDLDKHGALTSSNTPTNNAACAWLEAQEEEEVGFPVRPQVPLRPMTYKAAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGTRYPLCFGWCFKLVPVDPREVEEANEGENNCLLHPMSQHGMEDEEREVLRWKFDSSLARRHLARELHPEYYKDC"
  },
  {
    "reference": "MSTGNVYQELIRRYLVVVKKLYEEPIPQTARQRRRRKQQLRTRRAQLRELEGRILKQILDRGPDQLCQGVTNLALAEKSESSN",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that This function is essential since most viral proteins are translated from unspliced or partially spliced pre-mRNAs which cannot exit the nucleus by the pathway used by fully processed cellular mRNAs .; These pre- mRNAs carry a recognition sequence called Rev responsive element (RRE) located in the env gene, that is not present in fully spliced viral mRNAs (early transcripts)2. The designed protein should contain at least one Nuclear localization signal and RNA-binding (RRE), Nuclear export signal motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "MSVLSGR",
    "response#2": "MTRILLASRNPHKVAEFRELL",
    "response#3": "MR"
  },
  {
    "reference": "MAALLLLPLLLLLPLLLLKLHLWPQLRWLPADLAFAVRALCCKRALRARALAAAAADPEGPEGGCSLAWRLAELAQQRAAHTFLIHGSRRFSYSEAERESNRAARAFLRALGWDWGPDGGDSGEGSAGEGERAAPGAGDAAAGSGAEFAGGDGAARGGGAAAPLSPGATVALLLPAGPEFLWLWFGLAKAGLRTAFVPTALRRGPLLHCLRSCGARALVLAPEFLESLEPDLPALRAMGLHLWAAGPGTHPAGISDLLAEVSAEVDGPVPGYLSSPQSITDTCLYIFTSGTTGLPKAARISHLKILQCQGFYQLCGVHQEDVIYLALPLYHMSGSLLGIVGCMGIGATVVLKSKFSAGQFWEDCQQHRVTVFQYIGELCRYLVNQPPSKAERGHKVRLAVGSGLRPDTWERFVRRFGPLQVLETYGLTEGNVATINYTGQRGAVGRASWLYKHIFPFSLIRYDVTTGEPIRDPQGHCMATSPGEPGLLVAPVSQQSPFLGYAGGPELAQGKLLKDVFRPGDVFFNTGDLLVCDDQGFLRFHDRTGDTFRWKGENVATTEVAEVFEALDFLQEVNVYGVTVPGHEGRAGMAALVLRPPHALDLMQLYTHVSENLPPYARPRFLRLQESLATTETFKQQKVRMANEGFDPSTLSDPLYVLDQAVGAYLPLTTARYSALLAGNLRI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have very long-chain fatty acid-CoA ligase activity and be active in endoplasmic reticulum, mitochondrion.2. The designed protein should have a high specificity for ATP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "FRFESYSTVSKRCHNIGSGILSLVNTKRKRPLEANDFVVAILSHNNPEWILTDLACQAYSLTNTALYETLGPNTSEYILNLTEAPILIFAKSNMYHVLKMVPDMKFVNTLVCMDELTHDELRMLNESLLPVKCNSLNEKITFFSLEQVEQVGCFNKIPAIPPTPDSLYTISFTSGTTGLPKGVEMSHRNIASGIAFAFSTFRIPPDKRNQQLYDMCFLPLAHIFERMVIAYDLAIGFGIGFLHKPDPTVLVEDLKILKPYAVALVPRILTRFEAGIKNALDKSTVQRNVANTILDSKSARFTARGGPDKSIMNFLVYHRVLIDKIRDSLGLSNNSFIITGSAPISKDTLLFLRSALDIGIRQGYGLTETFAGVCLSEPFEKDVGSCGAIGISAECRLKSVPEMGYHADKDLKGELQIRGPQVFERYR",
    "response#2": "QPYKWLSYKQVSDRAEYLGSCLLQKGYKPSQEHFVGIFAQNRPEWVISEFACYTYSMVAVPLYDTLGEDAVIYIVNKADIATVICDTPQKALALIKNVEKGLTPGLKLIILMDPFEENLKNRGEKCGVEILSLLDAENLGKENFKKPVPPAPEDLSIICFTSGTTGDPKGAMLTHENIVSNAAAFLKCVEYTFEPSSEDVTISYLPLAHMFERVVQAVVYSCGARVGFFQGDIRLLPDDMKTLKPTVFPAVPRLLNRVYDKVQNEAKTPLKKFLLNLAVACKFSEVKKGIIRRNSVWDKLVFAKIQESLGGKVRFMVTGAAPISSPVLTFLRAAMGCPVYEAYGQTECTAGCTITSPGDWKSGHVGVPIACNHVKLEDVADMNYFAVNNEGQICIKGTNVFKGYPYRWLSYKQVSDRAEYLGSCLLYKGYKPSPDQFVGIFAQNRPEWIIAELACYTYSMVAVPLYDTLGAEAIVYIVNKAEIATVICDTPQKANVLLENVEKGLTLGLKMIILMDPFEDSLKEKGEKLGVDILSLYDAETLGKENFKKPVPPKPEDLSIVCFTSGTTGDPKGAMLTHRNVVANSASFLKCVENVFMPTCEDVTISYLPLAHMFERVVQAVVYTVGAKVGFFQGDVRLLPDDMKTLQPTVFPVVPRLLNRLYDKVHSEAKTPFKKFMLNLAISAKFSEVKQGIVRQDSIWDTLIFKKVQAGVGGKVRLMVTGAAPISSPVLVFLRAALGCPVYEAYGQTECTAGCTFSSPGDWTTGHVGAPLACNEVKLEDVVDMNYFSVDGEGEICIRGTNVFKGY",
    "response#3": "QPYKWLSYKQVSDRAEYLGSCLLQKGYKPSQEHFVGIFAQNRPEWVISEFACYTYSMVAVPLYDTLGEDAVIYIVNKADIATVICDTPQKALALIKNVEKGLTPGLKLIILMDPFEENLKNRGEKCGVEILSLLDAENLGKENFKKPVPPAPEDLSIICFTSGTTGDPKGAMLTHENIVSNAAAFLKCVEYTFEPSSEDVTISYLPLAHMFERVVQAVVYSCGARVGFFQGDIRLLPDDMKTLKPTVFPAVPRLLNRVYDKVQNEAKTPLKKFLLNLAVACKFSEVKKGIIRRNSVWDKLVFAKIQESLGGKVRFMVTGAAPISSPVLTFLRAAMGCPVYEAYGQTECTAGCTITSPGDWKSGHVGVPIACNHVKLEDVADMNYFAVNNEGQICIKGTNVFKGYYKRPEETAKVLRDGWFYSGDLGHVDDDGYFYIVDRKKDIIIAGGYNVYPREEIEEFLYQHPAVLDVQVVGVPDAKFGEEAAAWIKLKDGKSVSPDELKAYCKGKIARHKIPRYVIFTDDYPMTASGKILRRVLREEEKAKG"
  },
  {
    "reference": "MRALVVLGATLAVVCTAVFFPSVLRGKDLLWGKTEESHVISILEESKLMVDNAIYNTMKRNLNKREIVSPSQLLSFSKLPEPTSQAVSRAAEIMEASVQAMKRRGYLKLKPPTDILSEDLLNTIANMSGCLPYMLPPKCPHTCLANKYRLITGACNNRDHPRWGASNTALARWLPPTYEDGISEPRGWNPHFLYNGFPLPPVSTSERRLDGVGISSPFSAVWTSHTVTRWSLSQVREVTRQVIQVSNEAVTEDDQYSDLLVVWGQYIDHDIALTPQSTSRAAFGGGADCQLTCQNQSPCFPIQLPADASLTSGPACLPFYRSSAACGTGTQGAFFGNLSTANPRQQMNGFTSFLDASTVYGSSPASEKQLRNWTSAEGLLRVNTRHQDAGRAYLPFVSPPAPWACAPEPGADPAARAPCFLAGDGRASEVPSLAAVHTLWLREHNRLAAALKALNAHWSADTAYQEARKVVGALHQIITMRDYVPRILGPEAFRQHVGPYTGYDPTVDPTVSNVFSTAAFRFGHATVHPLVRRLDARFQEHPALPRLWLRDAFFSPWRLLGEGGLDPLLRGLLARPAKLQVQDQLMNEELTERLFVLGNSGTLDLASLNLQRGRDHGLPGYNDWREFCGLPRLETQANLSTAIANRGVADRLMDLYKHPDNIDVWLGGLAENFLPRARTGPLFACIIGKQMKALRDGDRFWWENHHVFTEAQRRELERHSLSRVICDNTGLPRVPADAFRVGSFPQDFESCENIPGMNLEVWREAFPQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein with iodide peroxidase activity, heme binding, calcium ion binding is needed to enhance thyroid hormone generation, hormone biosynthetic process, response to oxidative stress, hydrogen peroxide catabolic process in a specific context.2. A protein should be able to manipulate the activity of enzymes in the Hormone biosynthesis pathway, either by activating or inhibiting their function.3. The designed protein should be able to compete with endogenous proteins or inhibitors for heme b binding.4. The protein must exhibit the following characteristics: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4)..5. The protein contains novel Sushi, EGF-like domains that confer a unique function or activity.6. The protein should be designed to have high stability and activity towards [thyroglobulin]-L-tyrosine + H(+) + H2O2 + iodide =   [thyroglobulin]-3-iodo-L-tyrosine + 2 H2O. The designed protein sequence is ",
    "response#1": "YDGWYNNLAYHRRGTAGAPLMRFLPARYSDGAYQPLRGLPNARKISNAATRGDSALPSQRNLTVLSVYFGYHVLFEIAESRRPGCPPEFMHIPVSADDPVFGSNSTNEKVLLPFQRAQWDHDTGKSPNNPRTQINHVTAWIDGSSIYGSSSSWCDALRSFQGGLLLAGSDDGIPKQSDSNYFMWSAPNPCNGQTGPEGLYEFGNSWANENIFTVAEGIIWFRYHNYLASQLQKEHPSWSDEELFQHARKRVIATFQNIAFYEWLPAYLAVSAPQYPGYQKYVDPGVSPEFLAAVVRFGLSLVPPGVYMRNRTCHYRSIMNSDGSRSPALRLCNSFWNRNNANLQSAEDVDDLLMGMASQIAEREDNIIVEDLRDYMYGTLQFSRSDAVALTIQRGRDFGLPSFNQVREALNMPPVQTWVEINPKLNQTNPQLFKDLAELYGNDISKLELFVGALLESDGKTGDLVTSIYLDQFERIRNADRFWFENKQNGLFTEEEIQTIRNTTYHDILLAVTSAQQGDIQENVFFWLNGDPCPQPQQVSESDLEPCTKAISISYGDIDECARGDLNNCQEMCVNSVGSFMEYDGWYNNLAYHRRGAAGAPFMRLLPARYSDGSYQPLQELPNARLISNAAMSGDPALLSIRNQTVLSVFFGYHVVLEIAESRIPGCPPEFMHIPVSPNDPVHGSNSSTHRVLLPFQRAQWDQSTGKSPNNPRTQVNHVTAWIDGSSIYGSSSSWSDALRSFQGGLLSSGSDKNMPKQSDNNYFMWSAPNPCNGETGPEGLYEFGNAWANENIFTVAEGIIWFRYHNYLASQLHEDHPSWSDEELFQNARKRVIATFQNIAFYEWLPAYLGVLVPPYPGYQKYVDPGVSPEFQAAAVRFGLTLVPPGVYMRNRTCHYRSVVNFDGRTSPAVRLCNSFWNRNNPNLKSAQDVDDLIMGMASQIAEREDNVIVEDLRDYMYGTLKFSRSDAAALTIQRGRDFGLPSYNEVREALNLSPVQTWGEINPKLNQTNPQLFKDLSELYKNDISKLELFVGALLEGDGGPGPVVTSIFLDQFDRIRNADRFWFENKQNGLFTEEEIQSIRNTTYYDILLAITSAESEDIQKNVFFWLNGDPCPQPQPVTASDLQPCTKATSMSYFDT",
    "response#2": "YDGWYNNLAYHRRGAAGAPFMRLLPARYSDGSYQPLQELPNARLISNAAMSGDPALLSIRNQTVLSVFFGYHVVLEIAESRIPGCPPEFMHIPVSPNDPVHGSNSSTHRVLLPFQRAQWDQSTGKSPNNPRTQVNHVTAWIDGSSIYGSSSSWSDALRSFQGGLLSSGSDKNMPKQSDNNYFMWSAPNPCNGETGPEGLYEFGNAWANENIFTVAEGIIWFRYHNYLASQLHEDHPSWSDEELFQNARKRVIATFQNIAFYEWLPAYLGVLVPPYPGYQKYVDPGVSPEFQAAAVRFGLTLVPPGVYMRNRTCHYRSVVNFDGRTSPAVRLCNSFWNRNNPNLKSAQDVDDLIMGMASQIAEREDNVIVEDLRDYMYGTLKFSRSDAAALTIQRGRDFGLPSYNEVREALNLSPVQTWGEINPKLNQTNPQLFKDLSELYKNDISKLELFVGALLEGDGGPGPVVTSIFLDQFDRIRNADRFWFENKQNGLFTEEEIQSIRNTTYYDILLAITSAESEDIQKNVFFWLNGDPCPQPQPVTASDLQPCTKATSMSYFDTCVNTKGSYRCLDINECKDLMDPPCHLSAECKNIKGSFQCLCTDPYMLGEDERTCLDINECENEVDPPCHASARCKNTKGSFQCECSDPHVPGEDGRTCEDIDECARGDLNNCQEMCVNSVGSFMCACPDGFMLNDDGVSCDACGFPDPVENGGFLLCEELGKRVLVFSCRHGFRLRGPEQITCSPRGWDSPPPSCK",
    "response#3": "YDGWYNNLAYHRRGTAGAPLMRFLPARYSDGAYQPLRGLPNARKISNAATRGDSALPSQRNLTVLSVYFGYHVLFEIAESRRPGCPPEFMHIPVSADDPVFGSNSTNEKVLLPFQRAQWDHDTGKSPNNPRTQINHVTAWIDGSSIYGSSSSWCDALRSFQGGLLLAGSDDGIPKQSDSNYFMWSAPNPCNGQTGPEGLYEFGNSWANENIFTVAEGIIWFRYHNYLASQLQKEHPSWSDEELFQHARKRVIATFQNIAFYEWLPAYLAVSAPQYPGYQKYVDPGVSPEFLAAVVRFGLSLVPPGVYMRNRTCHYRSIMNSDGSRSPALRLCNSFWNRNNANLQSAEDVDDLLMGMASQIAEREDNIIVEDLRDYMYGTLQFSRSDAVALTIQRGRDFGLPSFNQVREALNMPPVQTWVEINPKLNQTNPQLFKDLAELYGNDISKLELFVGALLESDGKTGDLVTSIYLDQFERIRNADRFWFENKQNGLFTEEEIQTIRNTTYHDILLAVTSAQQGDIQENVFFWLNGDPCPQPQQVSESDLEPCTKAISISYSS"
  },
  {
    "reference": "MRRGKRPRWMLKIAEERIDILFRMADREFSANPHRSHRYTELARNIAMKYRVRIPREWRRRFCRKCYSFLKPGANCTVRIADGKVNFRCHECGHIMRFPYIREKKDRRRNKIESHTTKEGTDEQITVGAHNKCGESQSDR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be designed to catalyze the following reaction Endonucleolytic cleavage of RNA, removing 5'-extranucleotides   from tRNA precursor..2. The ideal protein would be one that plays a critical role in tRNA 5'-leader removal through zinc ion binding, ribonuclease P activity within cytoplasm, ribonuclease P complex.3. The designed protein should have a binding site that is specific to the target Zn(2+) ligand.4. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation.5. The protein must exhibit the following characteristics: Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.. The designed protein sequence is ",
    "response#1": "SLAGRYSVLMPNTARGGGISRKITNLQDRKRLKEIARDLEVPQGMGVILRTAGANRTRVEVKRDFEYLMRLWENVRTLTLQSTAPTLVYEEGSLIKRSIRDLYNKDISEIVVAGEEGYREAKAFMKMLMPSHAKVVQPYRDVHPIFSRSGIEAQLDRMLQPQVTLKSGGYIIINQTEALVSIDVNSGRSTREHSIEETALQTNLEAAEEVARQLRLRDLAGLVVIDFIDMEEKRNNRAVEKRLKDHLKNDRARIQVGRISHFGLLEMSRQVCSHCNGTGHVRSQSSVALHVLRGIEEYLLKNTTHNISVRTTPDIALYLLNHKRGTIIDYEGRFGVAILIEADAHVGAQHFAIDWVSLAGRYSVLMPNTARGGGISRKITNLQDRKRLKEIARDLEVPQGMGVILRTAGANRTRVEVKRDFEYLMRLWENVRTLTLQSTAPTLVYEEGSLIKRSIRDLYNKDISEIVVAGEEGYREAKAFMKMLMPSHAKVVQPYRDVHPIFSRSGIEAQLDRMLQPQVTLKSGGYIIINQTEALVSIDVNSGRSTREHSIEETALQTNLEAAEEVARQLRLRDLAGLVVIDFIDMEEKRNNRAVEKRLKDHLKNDRARIQVGRISHFGLLEMSRQVCSHCNGTGHVRSQSSVALHVLRGIEEYLLKNTTHNISVRTTPDIALYLLNHKRGTIIDYEGRFGVAILIEADAHVGAQHFAIDWVCSHCNGTGHVRSQSSVALHVLRGIEEYLLKNTTHNISVRTTPDIALYLLNHKRGTIIDYEGRFGVAILIEADAHVGAQHFAIDW",
    "response#2": "SLAGRYSVLMPNTARGGGISRKITNLQDRKRLKEIARDLEVPQGMGVILRTAGANRTRVEVKRDFEYLMRLWENVRTLTLQSTAPTLVYEEGSLIKRSIRDLYNKDISEIVVAGEEGYREAKAFMKMLMPSHAKVVQPYRDVHPIFSRSGIEAQLDRMLQPQVTLKSGGYIIINQTEALVSIDVNSGRSTREHSIEETALQTNLEAAEEVARQLRLRDLAGLVVIDFIDMEEKRNNRAVEKRLKDHLKNDRARIQVGRISHFGLLEMSRQIXXIXTXR",
    "response#3": "SLAGRYSVLMPNTARGGGISRKITNLQDRKRLKEIARDLEVPQGMGVILRTAGANRTRVEVKRDFEYLMRLWENVRTLTLQSTAPTLVYEEGSLIKRSIRDLYNKDISEIVVAGEEGYREAKAFMKMLMPSHAKVVQPYRDVHPIFSRSGIEAQLDRMLQPQVTLKSGGYIIINQTEALVSIDVNSGRSTREHSIEETALQTNLEAAEEVARQLRLRDLAGLVVIDFIDMEEKRNNRAVEKRLKDHLKNDRARIQVGRISHFGLLEMSRQRYAGVGN"
  },
  {
    "reference": "MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAIRGMINAVSFMVKVEE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be involved in ribosomal large subunit assembly, cytoplasmic translation by localizing to cytoplasm, cytosolic large ribosomal subunit and exhibiting structural constituent of ribosome.2. The protein design should prioritize the formation of strand-strand-helix-strand-helix-turn-strand elements. The designed protein sequence is ",
    "response#1": "ETHGELGEGARKFVFFNIPQIQYKNPWVQIMLFKNMTPTPFLRFYLDSGEQVLVDVETKSNKEIMEHVKKILGKQ",
    "response#2": "SVMTLRGHANKVVSLSPSPENEYSLVSGSHDGTCRVWR",
    "response#3": "MPIQVGNALPLRFIPVGTVVHAVELEPKKGAKLARAAGTSAQIQGREGDYVILRLPSGELRKVHGECYATVGAVGNADHKNIVLGKAGRSRWLGRRPHVRGAAMNPVDHPHGGGEGRAPRGRPPASPWGWG"
  },
  {
    "reference": "MPVYNKEELVRFVEEAKQYARYGKVADYIPALGKANPNELSIAVYTADGKVVDAGDVTVKVTLQSISKILALALVLIDRGEDEVFRKVGMEPTGDPFNSIAKLEEVQPSKPLNPMINAGALAVTHMIRGRSVEERLERLLAFIRRLAGNEQITYSEEVAQSEFETAFLNRSLCYFLKQHGIIDEDVEELMDLYTKQCAVEMTCIDLARIGLVFALDGRDPHSGELLMPLDVARICKTFMVTCGMYNASGEFAIKIGIPAKSGVSGGILAAVPGRCGIGIFGPALDDKGNSLTGVKLLERLSKTYSLSIF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be able to bind substrate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "YQIGEALIGNGNELAHIDLVIGDKNGPVGTAFVNNMSNLSLGHTPLLSVIRPNLMTKPATLIVPKVSVRDLDDANKIFGPAQTAVGRAVADAVEEGILPAEEAENMVLIISVFIHPEASDFRKIYQYNYGATKLALRRAMEGYPSVNKVLAEKDRGAHPW",
    "response#2": "IGEALIGNGNELAHIDLVIGDKNGPVGTAFVNNMSNLSLGHTPLLSVIRPNLMTKPATLIVPKVSVRDLDDANKIFGPAQTAVGRAVADAVEEGILPAEEAENMVLIISVFIHPEASDFRKIYQYNYGATKLALRRAMEGYPSVNKVLAEKDRGAHPVL",
    "response#3": "IGEALIGNGNELAHIDLVIGDKNGPVGTAFVNNMSNLSLGHTPLLSVIRPNLMTKPATLIVPKVSVRDLDDANKIFGPAQTAVGRAVADAVEEGILPAEEAENMVLIISVFIHPEASDFRKIYQYNYGATKLALRRAMEGYPSVNKVLAEKDRGAHPVR"
  },
  {
    "reference": "MKAKLNTITEAILEIKKGNLVIVVDDENRENEGDFVAAAEKVTPEMINFMATNGRGLICTPLIEERCEELNLDLMVGKNTDPMKTQFTVSVDKIGDGCTTGISAHDRCKTVKALVNPKTKTDELARPGHIFPLKAMNGGVLRRAGHTEAAIDLARLANLKPCGLIVEVMNKDGSMAKLPDLFKIAKKYKLKIISIEDLIAYRLKTESLINKEVEIDLPTKYGKFKLIAYKQTNTNEEHLAIVKGEWKQDEPVLTRVHSSCFTGDILGSLKCDCGDQLTLALQQIEKEGKGIVLYMNQEGRGIGLLNKLKAYHLQAQGLDTVEANTKLGFKMDQRDYGVGAQILRDLGASKLKLLTNNPTKRTGLISYGIEIVENIPILAEINDYNKKYLETKREKMNHNI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must possess 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, zinc ion binding, manganese ion binding, magnesium ion binding, GTP binding to support riboflavin biosynthetic process under certain conditions.2. The protein contains novel GTP cyclohydrolase II domains that confer a unique function or activity.3. Zn(2+) is required for the proper folding and stability of the protein.4. The Proton acceptor; for GTP cyclohydrolase activity active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.5. The D-ribulose 5-phosphate binding site should be stable and able to withstand changes in environmental conditions.6. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "RVSVNLPTLYGKFVLHLYRSFVNEYLHLALCLGEIGSLDNSPAPIQTEPVLIRVHDECLTGDIFGSSRCDCGEQLHNALKMIQQEGKGILLYMRQEGRGIGLENKLHAYALQENGFDTVEANKKLGFVADKRDYGIGAQILRDLGVSKMRLLSNNPKKFTALAGYGLEIVERIASRFSWGQRVVETRLPNRYGTWRAIGFAAPHGGGEHLALVHGDLGDGEDVLVRAHSECLTGDVLGSERCDCGTQLDAAMAAIAEEGRGVVLYLRGHEGRGIGLLAKLQAYALQDTGVDTVDANLELGLPADAREYGDAAQMLDDLGVRSARVLTNNPAKLDGLARFGLQVRGRE",
    "response#2": "VSVQMPTKYGNFELIAFKQVNTGEIHMALKKGEWEKGEPVLVRVHSSCVTGDILHSLRCDCGEQLAAAMKMVEKEGKGLILYMNQEGRGIGLMNKLKAYKLQEEGRDTVEANLELGFQMDERDYGVGAQILRHLNICKLRLITNNPRKRAGLLGYGLEVVENVIGNKDPNIYTDIDRAAEAYLPTKFGGDFKAIAYTNEVDAHEHLALVKGEISPEEPVLVRVHSECLTGDVLGSERCDCGEQLKGAMQMIKKEGKGVLVYMHQEGRGIGLANKLKAYSLQDTGLDTVEANERLGFKADLRDYGIGAQILLDIGVKKMRIITNNPKKIVGLEGYGLEVTERVEVSMPTKYGDFRIIPFRQKSNGAEHVALIKGEWKPDEEILVRMHSSCMTGDIFGSFRCECGDQLHKSMELIEKAGKGVVVYMMQEGRGIGLMNKIAAYKLQDEGLDTIDANIHLGFQADERDYGVGAQILRDVGVSKMKLLTNNPVKRVGIEGYGLTVTEIINDGPVTFRVSVNLPTLYGKFVLHLYRSFVNEYLHLALCLGEIGSLDNSPAPIQTEPVLIRVHDECLTGDIFGSSRCDCGEQLHNALKMIQQEGKGILLYMRQEGRGIGLENKLHAYALQENGFDTVEANKKLGFVADKRDYGIGAQILRDLGVSKMRLLSNNPKKFTALAGYGLEIVERIEVSMPTKYGDFRIIPFRQKSNGAEHVALIKGEWKPDEEILVRMHSSCMTGDIFGSFRCECGDQLHKSMELIEKAGKGVVVYMMQEGRGIGLMNKIAAYKLQDEGLDTIDANIHLGFQADERDYGVGAQILRDVGVSKMKLLTNNPVKRVGIEGYGLTVTEII",
    "response#3": "AAVHMPTKYGDFTAYGYVNRLNGEHHVALVKGDIGDGEDVLCRVHSECLTGDTFGSLRCDCGQQLAAAMSQIEKEGRGILLYMRQEGRGIGLINKLKAYALQEQGMDTLEANLALGFAADQREYYIGAQILRDLGVKSMRLLTNNPDKLYQLAEFGMHINSRVRHPFPGPGLGVRILGEVKKEYADILRRADAIFIAELEKNFDEDTSAEGAQDPSMVGKAWEQGDHPWHSLDRHQAVFPWERDASARMPTKYGDFQIYGYVNDITGEHHVALVKGDLGDGENVLCRVHSECLTGDTFGSRRCDCGEQLQRAMQQVEKEGRGIILYMRQEGRGIGLINKLKAYELQEQGLDTVEANIRLGFDADLREYWVGAQILADLGARSLRLLTNNPDKIYGLEGFGLTITERVWE"
  },
  {
    "reference": "MEITNPILTGFNPDPSLCRQGEDYYIATSTFEWFPGVRIYHSRDLKNWSLVSTPLDRVSMLDMKGNPDSGGIWAPCLSYADGKFWLLYTDVKIVDSPWKNGRNFLVTAPSIEGPWSEPIPMGNGGFDPSLFHDDDGRKYYIYRPWGPRHHSNPHNTIVLQAFDPQTGTLSPERKTLFTGTPLCYTEGAHLYRHAGWYYLMAAEGGTSYEHAVVVLRSKNIDGPYELHPDVTMMTSWHLPENPLQKSGHGSLLQTHTGEWYMAYLTSRPLRLPGVPLLASGGRGYCPLGRETGIARIEWRDGWPYVEGGKHAQLTVKGPQVAEQPAAVPGNWRDDFDASSLDPELQTLRIPFDDTLGSLTARPGFLRLYGNDSLNSTFTQSTVARRWQHFAFRAETRMEFSPVHFQQSAGLTCYYNSKNWSYCFVDYEEGQGRTIKVIQLDHNVPSWPLHEQPIPVPEHAESVWLRVDVDTLVYRYSYSFDGETWHTVPVTYEAWKLSDDYIGGRGFFTGAFVGLHCEDISGDGCYADFDYFTYEPV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in xylan catabolic process, potentially with improved properties such as stability, specificity or selectivity.2. The Proton donor, Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "GGYYYSWWTDGAGDATYQNNGGGSYTLTWSGNNGNLVGGKGWNPGAASRSISYSGTYQPNGNSYLSVYGWTRSSLIEYYIVESYGSYDPSSAASHKGSVTCNGATYDILSTWRYNAPSIDGTQTFEQFWSVRNPKKAPGGSISGTVDVQCHFDAWKGLGMNLGSEHNYQIVATEGYQSSGTATIR",
    "response#2": "GGYYYSWWTDGAGDATYQNNGGGSYTLTWSGNNGNLVGGKGWNPGAASRSISYSGTYQPNGNSYLSVYGWTRSSLIEYYIVESYGSYDPSSAASHKGSVTCNGATYDILSTWRYNAPSIDGTQTFEQFWSVRNPKKAPGGSISGTVDVQCHFDAWKGLGMNLGSEHNYQIVATEGYQSSGTATIF",
    "response#3": "GGYYYSWWTDGAGDATYQNNGGGSYTLTWSGNNGNLVGGKGWNPGAASRSISYSGTYQPNGNSYLSVYGWTRSSLIEYYIVESYGSYDPSSAASHKGSVTCNGATYDILSTWRYNAPSIDGTQTFEQFWSVRNPKKAPGGSISGTVDVQCHFDAWKGLGMNLGSEHNYQIVATEGYQSSGTATIY"
  },
  {
    "reference": "MASESSIPLYDCLIIGGGIAGLSSALSLVRTLHTAVVFDEGIHRNDQAPHLATVPTWDSQDPKRFRDAAKLNILSKYSTVEFANVKLEKFNKALGKPFLTKVFGHDYVVLPSKYFDDIKRASPQSLSFFQALSDGLNMEASVGHLYASTTEIDVVVKHLNPRLTQLTPLLCDEAEYAIEREVGALPDWKKFNVSNLIAAIVHRTTNRILVGKELCRNEEYLAITTKFSRSLFISGIFWNFVRLGPLRKLVAWLTIGLHLRDRNAAAKVLLPHVLARRQEKEAGVDVATKYPDALQWTIDTAPSFPGDDEPLHQVYHMLHLTFAASSASGVGVTQCLLNVLAYPEYLEPLRDEISTVVARHGGWTDKALSQMALLDSFIRETMRLHPAGSFMDDHFRFHDGLTLPKGTNIIAPALAIHYDPDNYEDAHRFDGFRFARYRQKQGENHRWLASTIDQKFLQFGYGNHACPGRFYAIRKIKLVLAKLIMDYDFKWAQPRPVHDRPEDFAIEAQLVAAPDAEILIRSRNLSN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that In addition, oxygenases (aclB, aclC, aclL and aclO) and O-methyltransferases (aclM and aclU) act as tailoring enzymes to produce the intermediate dechloroaspirochlorine; Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of aspirochlorine (or antibiotic A30641), an unusual halogenated spiro compound with distinctive antifungal properties due to selective inhibition of protein biosynthesis, and which is also active against bacteria, viruses, and murine tumor cells The designed protein sequence is ",
    "response#1": "FESAVSRNPMDVAVQKGNCHLTYQELDTHAGRVAATLRGYIQDGDVVCLHADRSVNWIVGIMGILKAGGVYCALDKALPQEARETIFSASGSRLFLVPSLSDQSFCPTDCDRLLVVEDLVKDDNVPITHRDSPRPQTDAYLCFTSGSTGKPKGVMCLHQGLVAFQRDLEVRLFAQPGRRVAQIMSVAFDGSIHEIFSALSYGAALVLQSGDDPFAHLSDVDSAILTPSMARVLNPADFERLSTVSTKVYLVGEPVTQDVCDRWSEQKTLYNMYGPTEGTCGATIKQLHPRQRVTIGPPNPSTRIYIMNQHQELVPPGVIGEIYIAGVQVARHR",
    "response#2": "FESAVSRNPMDVAVQKGNCHLTYQELDTHAGRVAATLRGYIQDGDVVCLHADRSVNWIVGIMGILKAGGVYCALDKALPQEARETIFSASGSRLFLVPSLSDQSFCPTDCDRLLVVEDLVKDDNVPITHRDSPRPQTDAYLCFTSGSTGKPKGVMCLHQGLVAFQRDLEVRLFAQPGRRVAQIMSVAFDGSIHEIFSALSYGAALVLQSGDDPFAHLSDVDSAILTPSMARVLNPADFERLSTVSTKVYLVGEPVTQDVCDRWSEQKTLYNMYGPTEGTCGATIKQLHPRQRVTIGPPNPSTRIYIMNQHQELVPPGVIGEIYIAGVQVARHR",
    "response#3": "PQVLKMKEILRSGSLGRILATNLLVTDDLFLKFHADKRHSHDKTNGANIVTIAGGHLLDAMAFLLGEFTTLHAHTSMLFPKPVLCDTDGNLKTGQFNDSPDTFTMHGKIGISEVPVSVCMYSHPPTTPNLFQWVITGEKGSLKMEL"
  },
  {
    "reference": "MMKSSFLIVPILALTLPFLGAEEQNQEKLTRCESDKRLFNEEKVKYIPIYYMLNRFPSYGFFYQHRSAVSPNRQFIPYPYYARPVVAGPHAQKPQWQDQPNVYPPTVARRPRPHASFIAIPPKKNQDKTAIPAINSIATVEPTIVPATEPIVNAEPIVNAVVTPEASSEFLITSAPETTTVQVTSPVV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in lactation, potentially with improved properties such as stability, specificity or selectivity.2. For general function, the protein need meet that Kappa-casein stabilizes micelle formation, preventing casein precipitation in milk. The designed protein sequence is ",
    "response#1": "TTTTSSPSSDIRDKFIFGNLEVKVKEVKGCQLHFLNVKCELALKKTEIKTKPLANHTFFDVFSFRVSAATSELEIEAWKKNFLFKDKMTGSLTIPINDLLHANGEAKWR",
    "response#2": "PPGKPEILKCRSPEKETFTCWWKPGDDGGLPTNYTLTYHKEGETLTHECPDYITSGPNSCYFNKKHTSIWTMYIITINATNQMGSSSSDPRYVDVTYIVEPK",
    "response#3": "MEEGEVFAIETFGSTGKGVVHDDMDCSHYMKNFDVGHVPIRLPRAKHLLNVVNEHFGTLAFCRRWLDRLGESKYLMALKNLCDLGIIDPYPPLCDAKGSYTAQFEHTILLRPTCKEVVALKFALKKDTMEYIRLATQVYCPPEFDKSGMYHGKPATVWSLGELMFELLCGDTPDSSDLDMIDAGIWSKPGLSKECCHLIQSCLQQKPKKLLSLGKIILHDWR"
  },
  {
    "reference": "MRPVHDVASIRAAEEALMARMPEGALMQRAATGLAATTAALMRETFGRVTGLRLAVVAGSGNNGGDALFAASQLARRGVSVDILPTSDTLHPDGLRAAQREGASIRAPRRADVVLDAVVGIGGTGPLRPAAAQIRDNVDAELTIAVDLPSGLRPDTGDVPGEVWVADHTVTFGTLKPGVVLRPDICGDLHLVDIGLDATLPRASIHLVEREDAGRYFPGPGFDDNKYTRGVVSIAAGSTKYPGAGQLCTAGARHADVGMVRSPVGGFPDVVAAEGRTDAYVVGPGLADQGRLHTAVSQALASGRPVVLDAEALTKVAAGPVVITPHEGEFARLGYSPGTDRIAAVRAAAAAMGVVVLLKGAVTVVAAPSGEVFINAHSSPNLAAAGSGDVLSGLMGGMLARHFVGRDPEHVEMARLAACAALVHGQAGALASFPATSLDIADRVAAAVAWAAD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be able to bind (6S)-NADPHX ligand in a variety of conditions2. The designed protein should have NADHX epimerase activity, ATP binding to facilitate nicotinamide nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "DSHKGSHGTVSIVGGCPGMNGAVLLAGRAALAGGAGKVHVLSLDPRLVVDTTSPELMLHAAALPPCLPNCDVLAVGPGLGQSDHAVQWLQQAVDSPQPLVLDADALNLLATDSGLQNRLHLRKAATVITPHPAEAARLLACSTAAIQADRLQAAQQLSMALNAVVVLKGAGSLICAPNRPYYLNTSGGPALAAAGQGDVLTGLIAALLAQGMPALAASCLAVRAHGLAGDQYQQEAGGPIGLTASVTALY",
    "response#2": "DSHKGSHGTVSIVGGCPGMNGAVLLAGRAALAGGAGKVHVLSLDPRLVVDTTSPELMLHAAALPPCLPNCDVLAVGPGLGQSDHAVQWLQQAVDSPQPLVLDADALNLLATDSGLQNRLHLRKAATVITPHPAEAARLLACSTAAIQADRLQAAQQLSMALNAVVVLKGAGSLICAPNRPYYLNTSGGPALAAAGQGDVLTGLIAALLAQGMPALAASCLAVRAHGLAGDQYQQEAGGPIGLTASVTALL",
    "response#3": "GPSIWLGAWPPLSTMGHKFSRGHAVVLSGPAGKSGAARLAAGAALRVGAGLVTVASPQGAVAEHAAQLNAVMIDSWRDVKGFRRLLSDLRRNAVCLGPGAGVGKQLRENVLTTLSLEKATVLDADALTSFAQARKSLFGKLHKNCILTPHQGEFVRLFGVDGDKLQMTRAAAAECGAVVLLKGPDTVIAAPDGRAIINTNAPPQLATAGSGDVLAGFCTGLLSQGVPAFEAAAVACWLHGAAAIQLGSGLIAEDLAGALPSVLQKR"
  },
  {
    "reference": "MTATGETPREQRFQRHAKERARPERKGKRKREGAGGGAKGKKSSGSRILVINAGSSSLRYALFAFPPATGTVLLKGYIDAIETGQRGRSRRQTTHAITLYKTSAGERRRSNRAQPVLKEQLQQQQTCKTPVRNHEEAIALALATLTETGALAAMSDITAVGHRVVHGGAVHAKPAKHPKQATLPAHRVTPALLRAVQQYNSFAPLHNPHNLAGIHKSLQHLPRAKHFIVFDTAFHRSIPDVAATYALPRSLAKKQGLRRYGFHGTSVAGAIREAKQLLGTMPKRAVVCHVGGGVSVTALLRGTSIDTSMGVTPLEGPPMGTRSGTVDPGLVIHLASQGVSASELLHLFSNESGLLGVSGVSADIREVLAAAKKGNKDAALSLALLEYHLAKHVSSYLPALGGLDTLIFTGGIGEHIASLRARVCERLAFLGVKLDRRKNDRHARIISTSSSRVSVLVIPADEERTIAQAVFSALSLC",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be able to bind Mg(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "LHDSYMSVKEALTHAGLSHDRDIDIRWVASEDIESDGSEAHLSDVTGIVVPGGFGYRGVEGMIDAVRYARENRIPYLGLCLGMQVMVIEAARNLLGKKDANSTEFNQETNNPVIDLMPDQVGVTDKGGTMRLGVYPCEITGVGVTSKAYAESDTVILERHRHRFEFNNSFRDQLAAVGLMATGLSPDGNLVEIVEHDGHPFMAGTQFHPEFLSRPSSPHPMFKEFIEYYARMTS",
    "response#2": "LHDSYMSVKEALTHAGLSHDRDIDIRWVASEDIESDGSEAHLSDVTGIVVPGGFGYRGVEGMIDAVRYARENRIPYLGLCLGMQVMVIEAARNLLGKKDANSTEFNQETNNPVIDLMPDQVGVTDKGGTMRLGVYPCEITGVGVTSKAYAESDTVILERHRHRFEFNNSFRDQLAAVGLMATGLSPDGNLVEIVEHDGHPFMAGTQFHPEFLSRPSSPHPMFKEFIENTK",
    "response#3": "LEIAVVGKYVDLHDSYMSVKEALTHAGLSHDRDIDIRWVASEDIESDGSEAHLSDVTGIVVPGGFGYRGVEGMIDAVRYARENRIPYLGLCLGMQVMVIEAARNLLGKKDANSTEFNQETNNPVIDLMPDQVGVTDKGGTMRLGVYPCEITGVGVTSKAYAESDTVILERHRHRFEFNNSFRDQLAAVGLMATGLSPDGNLVEIVEHDGHPFMAGTQFHPEFLSRPSSPHPMFKEFIAFTR"
  },
  {
    "reference": "MENRWQVMVVWQVDXMKIRTWNSLVKHHMYVSKKAKGWYYRHHYETKHPKTSSEVHIPVGXAXLVIVTYWGLTTGEQPWHLGHXVSIEWRQGKYKTQVDPEMADKLIHCYYFNCFTASAIRQAVLGRPVLPRCXYPAGHXQVGTLQYLAXTAXVGVKKRRPPLPSVTKLTEDRWNERQKTQGHRGNPIMNGP",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Seems to play a role in viral morphology by affecting the stability of the viral nucleoprotein core; Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G; May interact directly with APOBEC3G mRNA in order to inhibit its translation; In turn, they are directed to the 26S proteasome for degradation; Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions; Recruits an active E3 ubiquitin ligase complex composed of elongin BC, CUL5, and RBX2 to induce polyubiquitination of APOBEC3G and APOBEC3F; Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism.2. The protein can be targeted to host cell cytoplasm, virion component, host cell plasma membrane and perform RNA binding.3. The protein design must feature the HCCH motif motif. The designed protein sequence is ",
    "response#1": "MENRWQVMIVWQVDRMRIRAWNSLVKHHMYVSKKAKKWYYRHHYDITHPKISSEVHIPLGEAKLVITTYWGLHTGERDWHLGQGVSIEWRQRKYRTQVDPDLADQLIHLYYFDCFSESAIRNAILGHRVSPRCEYQAGHNKVGSLQYLALAALTTPKKTKPPLPSVKKLTEDRWNKPQKTKGHRGSHTMNGH",
    "response#2": "MENRWQVMIVWQVDRMRIRTWNSLVKHHMYVSKRAKGWSYRHHYESTHPKISSEVHIPLGDARLVVTTYWGLHTGERDWHLGQGVSIEWRKRRYSTQVDPDLADQLIHLYYFDCFSESAIRQAILGHRVSPRCEYQAGHNKVGSLQYLALKALTTPPRRRPPLPSVKKLTEDRWNKPQKTRGHRGSHTMNGH",
    "response#3": "MENRWQVMIVWQVDRMRIRTWKSLVKHHMYISKKAKKWFYRHHYESRHPKVSSEVHIPLGDARLVITTYWGLHTGERDWHLGQGVSIEWRKGKYNTQVDPDLADQLIHLYYFDCFSESAIRKAILGQLVSPRCEYQAGHNKVGSLQYLALVALITPKKIKPPLPSVRKLVEDKWNKPQKTRGRRGNHTMNGH"
  },
  {
    "reference": "MNTVKTVRELRAAVARARGEGKRIGFVPTMGNLHSGHAALVTKAAQRADFVVASIFVNPLQFGANEDLDKYPRTLAADQERLVQAGCNLLFAPTVEEMYPDGMSVQTRVSVPQLSEGLCGASRPGHFEGVATVVNKLFNMVQPDLAVFGEKDYQQLAVIRAMVRDLNMPIQIIGEPTVRAEDGLALSSRNGYLTPEQRTAAPALYRTLQHIAAGIGRGQRDFAALVAEGQAQLSAAGFRPDYLEVRHAVSLRPAVINDRDLVVIAAAYLGNTRLIDNLYLHLEEKTA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis pathway to avoid interference with native metabolic processes.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm that is critical for pantothenate biosynthetic process. The designed protein sequence is ",
    "response#1": "MRIILGVSAGIAAYKAAILLRLLREAGHAVRVVPTESSYAFVGRATWEALSGEPATHEVFDAVPEVAHVRLGQGADLVIVAPATADVLAKAATGRADDLLTTTLLTVRCPVLVAPAMHTEMWLHAATQANVATLRARGVHVLEPATGRLTGADSGPGRLPEPQEIAAAAIRAAIEA",
    "response#2": "RIVLGVTGGVAAYKAALLLRLFTEAGHDVRVVPTESALEFVGAPTWEALSGNPVQTGVFENVPEVPHVKLGQTADLVVVVPATANTLARAAHGLADDLLTNTLLTARCPVVMAPAMHTEMWEHPATVANVATLRDRGVLVIEPDSGRLTGADTGPGRLPEPDAIFAILARLDP",
    "response#3": "MRIILGVSAGIAAYKAAILLRLLREAGHAVRVVPTESSYAFVGRATWEALSGEPATHEVFDAVPEVAHVRLGQGADLVIVAPATADVLAKAATGRADDLLTTTLLTVRCPVLVAPAMHTEMWLHAATQANVATLRARGVHVLEPATGRLTGADSGPGRLPEPQEIAAAAILAAAAAVLRPPST"
  },
  {
    "reference": "MLRRPLLLVLFTVFSTLYAKDLRGVSPPTNESEAEVSPGDDEGPPEPGNEPDVNWRTLTIDPEDCKDKGKDCESLADEGNCLKKLNYAVGNCPWTCRFCKKENGDSKKCKDLAGERHCNGWKVKGDCVRLPDYMMQNCKLSCELCGPETRFKYTDEDVRCPEWAQAGYCSTNTDINLKCPHSCRKYKQRAPASTPYPYPVEALHPYQYRVVLPSVTILQTTTAAPSTQPAETTKAPPNTAAPTAAPTPAPTPAPAPAPTPAPVAPAPQTAPLAGSPPESTPEEQDDNSADESTEIEAGEGGGELCDEKHSSQQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein's functionality will benefit from Polar residues, Pro residues compositional bias in its structure.2. The protein contains novel ShKT domains that confer a unique function or activity.3. The protein can be expressed in nematocyst and perform toxin activity without triggering undesirable cellular responses.4. For general function, the protein need meet that Probable toxin probably only used for predation. The designed protein sequence is ",
    "response#1": "KGFPAEKGYGDSIAYVSEPLNGANAVKLTSYQVLQMEVTNQTTQKYRIRIRYATGGDTAASIWFHIIGPSGNDLTNEGHNFSSVSSRNKMFVQGNNGKYVLNILTDSIELPSGQQTILIQNTNSQDLFLDRIEFN",
    "response#2": "TLPMYTTGATLNLILHQGYIQFAERWKSVNYDESFINQTKVDLQRRIQDYSTTVSTTFEKFKPTLNPSNKESVNKYNRYVRSMTLQSLDIAATWPTLL",
    "response#3": "KGFPAEKGYGDSIAYVSEPLNGANAVKLTSYQVLQMEVTNQTTQKYRIRIRYATGGDTAASIWFHIIGPSGNDLTNEGHNFSSVSSRNKMFVQGNNGKYVLNILTDSIELPSGQQTILIQNTNSQDLFLDRIEFC"
  },
  {
    "reference": "MSTYTFVLFCLQICLIQNVYSQCLGRVGPGGPPVGPYGGPLGGPGYGPVGYGGCGGYGGSGIGNVAVAGELPVAGSSAVMGQVPVIGAVEFAGPACAVGSVSISGACGPTCGCGGSPYY",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Include a signal peptide as an essential component of the protein.2. For general function, the protein need meet that This protein is one of many from the eggshell of the silk moth.3. The designed protein must be able to regulate chorion-containing eggshell formation. The designed protein sequence is ",
    "response#1": "GYPAPPCPTNYLFSCQPNLAPAPCAQEAPAYGSAGAYC",
    "response#2": "GYPAPPCPTNYLFSCQPNLAPAPCAQEAPAYGSAGAYC",
    "response#3": "GYPAPPCPTNYLFSCQPNLAPAPCAQEAPAYGSAGAYC"
  },
  {
    "reference": "MSTPTSNIPDVQNSADTRHIAINKVGIKSIRHPIKVQDKSAGIQHTIAMFNMYVGLPHNFKGTHMSRFVEILNSHEREISVENFPTMLRDMVVKLEAETGHIEMNFPYFINKTAPVSGVQSLMDYDVTFIGDICHGEIATSVKVVVPVTSLCPCSKKISEYGAHNQRSHVTVTAKTNDFMWIEEIVQLVEQEASCELFGLLKRPDEKYVTERAYDNPKFVEDMVRDVAARLNAEARVDAYVVESENFESIHNHSAYALIENDKKNPLAHA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Converts GTP to 7,8-dihydroneopterin triphosphate.2. The protein should have a low Km value for GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate +   H(+).3. A protein with GTP cyclohydrolase I activity functions is required to enable 7,8-dihydroneopterin 3'-triphosphate biosynthetic process.4. A protein that is capable of catalyzing reactions within the 7,8-dihydroneopterin triphosphate biosynthesis, Cofactor biosynthesis, 7,8-dihydroneopterin triphosphate from GTP: step 1/1 pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "AGHMTEIMQLLNLDLSDDSLMETPHRIAKMYVDEIFAGLDYANFPKITLIENKMKVDEMVTVRDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQILTALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRQEFLRALA",
    "response#2": "AGHMTEIMQLLNLDLSDDSLMETPHRIAKMYVDEIFAGLDYANFPKITLIENKMKVDEMVTVRDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQILTALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRQEFLRALA",
    "response#3": "IEQAVRQILEAIGEDPNREGLLDTPKRVAKMYAEVFSGLNEDPKEHFQTIFGENHEELVLVKDIAFHSMCEHHLVPFYGKAHVAYIPRGGKVTGLSKLARAVEAVAKRPQLQERITSTIAESIVETLDPHGVMVVVEAEHMCMTMRGVRKPGAKTVTSAVRGVFKDDAAARAEVLEHLA"
  },
  {
    "reference": "MKRVNVIQAKAFLKVIALLVPLLLNANGPAFALTEQASTPPHDSVASVSAPTAEAAVAAHAHGEEKAGDVIMHHILDNDVFSFEPFGEVHLPKIPPIAGVDISITKHVVMLWVVSAILLILFSLVGSKYKKMTARQAPTGLVNAMEALVEFIRIDVAKANIGVGYEKYLNYLLTVFFFVLLCNLLGLVPYGATATGNINVTLTLATFTFFITQVAALKAHGIKGYLAHLTGGTHPALWIIMIPIEFIGLFTKPVALTIRLFANMTAGHIVILSLIFISFILQSYIVAVVMSVPFSIFIYLLELFVAFLQAFIFTMLSSLFIGLASAHEGHEEHEAGVAHH",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Include a signal peptide as an essential component of the protein.2. The protein can be expressed in plasma membrane, proton-transporting ATP synthase complex, coupling factor F(o) and perform proton-transporting ATP synthase activity, rotational mechanism without triggering undesirable cellular responses.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "LDV",
    "response#2": "MI",
    "response#3": "XAG"
  },
  {
    "reference": "MKLETLLKNCLPLKTRGNTDIEVSGVEYNSKKVKPGALFVAIPGFKTDGRAYVFQAVAQGASAVVFEGEFFENINLPQIRVANARSALALLSEVYFGYPSQKMYVAGVTGTNGKTTLTYLAESLWKEASYKTGVVGTINTRFGNKVWPSDQTTPESRDLQEIFLTMKQAQVSHVCMEVSSHALDLHRVDGLDFDAAVFTNLTQDHLDFHRDLENYYKAKEKLFVSCLNQSSKKNRVAIVYGDDVYGQKLLKILEQKKIPNISYGFTKINQTYPEKYSLSLDGFEGRLRTSLGVLDVCVPLMGLFNVANVLACVELGIHSGLNLKQIQKGLSGCKPPPGRLEKIANKAGLSVFVDYAHTPDALKNVLTTLKALAPGKIIAVFGCGGDRDPGKRPLMGYEVGSLADVAAVTSDNPRTENPETIIDAIVPGLNRAGMSPYSQAKGYLVEPDRKKAIEKALTLAKKDDVVLIAGKGHEDYQIIGTQKFPFSDQEIVREHLGPSTPLRDHPE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should contain one or more Mur ligase central, Mur ligase N-terminal catalytic, Mur ligase C-terminal domains that are essential for its function.2. The protein should have a low Km value for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.3. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.4. The ideal protein would be one that plays a critical role in cell wall organization, cell cycle, cell division through ATP binding, magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity within cytoplasm.5. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan. The designed protein sequence is ",
    "response#1": "MEIKRITCNSQDVQEGDVFIAIKGDRFDGHNFLNAAIARGAGMAIVERMSLVQDGFIQIKVNDTRRAVSILARNFYQNPSKKLKIIGVTGTNGKTTVAFMDKSILEAAGFNCGLIVTGTNGKTTTVTLLYSLFKRMGYKTGLLSTVVNRIHNETVTATHTTPNAIELNALLREMVDAGCMYCFMEVSSHAVHQKRIAGVRFAGAVFTNITHDHLDYHGTFDEYLKAKKGFFDMLSADAFALVNKDDYHGEEMVKDTKARVRTYAIKSMADFRARVVENQFSGLQLNIDGKDLWTKLIGSFNAYNILAVYTVEGITLDSRKVRPNWMFVAIKGLQSDGHDFINKAIELGADCIICEQIPSERHPHVTYVRVNDAAASLGVMAAELGIKQEEAIKALAEIQGAEGRFQYVKSSNDITAIVDYAHTPDALKNVLNTIRDIRTGNEKVITVVGCGGDRDKGKRPLMAGIACELSDKVIITSDNPRSEEPEAIIMDMMKGVESDQTRKVMSITNRREAIRAACMMAQPGDILLIAGKGHENYQEIKGVKYPFDDKIEIKKILEEI",
    "response#2": "MKLRDLVSKLSVSAVKGNMDVEIKSIENDNRKVSDGSLFICIKGYTVDGHDFAQKAYESGAVAVIAERSLSLEIKSIENDNRKVSDGSLFICIKGYTVDGHDFAQKAYESGAVAVIAERSLSLPDLPVIYVDNVRRAMGMAAANFYGNPSEKLKLIGVTGTNGKTTVAFMDKSILEAAGYKTGLIGTVTGTNGKTTITYLLEDIFKNANFKTGVIGTINYRVGENVMDSEQRTTPDPLTLNKLLRDMIEQSIPYCFVEVSSHGLEQGRVEDIKFAGGVFTNLTHEHLDYHKTVENYLKAKLKLFENLSQDSYAVINNDSRYRDELISRVDAKIITYGIEKESDVMAKDLRLDLNGVRCRIHAEDDVIDIKTSLIGKHNVYNILAAASVAYSLGISDEVIRSGVKSTFVPGGRFQYVKSSNDITAIVDYAHTPDALKNVLNTIRDIRTGNEKVITVVGCGGDRDKGKRPLMAGIACELSDKVIITSDNPRSEEPEAIIMDMMKGVESDQTRKVMSITNRREAIRAACMMAQPGDILLIAGKGDEDYQIIKGRKIPFDDREVVSRELKKIG",
    "response#3": "MQLRELVNGLDLVRVQGEPEVAITGLAVDSRDVKPGFLFAALPGARVHGGEFIQYALRMKAGAILTDAKGAEIAADELAASDAAVIVAQDPRQALAQTAARWFSAQPETVVAVTGTNGKTSVSTFCRQIWEELDLPGVNLGTTGVEGAWNHPLKHTTPEPITLHRVMAEAAQNGITHVAMEASSHGLDQRRLDGVILAAAGFSNFSQDHLDYHETFEAYFDAKMGLFQRVLSEDGVAVINLDDPKGKEVAAICTARGQEVIGVGRHTDARLRLTGQRFDATGQDLLFEWQGRARQARLELIGGFQAENVLLASGLALACGAEPDQIFDVLTQLESVSGRLEQVETGRDFHVVVDYAHKPDAVEAALSTLRPLTAQAGGRLICVIGAGGDRDTGKRPVMGEIAARLADLVIVTDDNPRSEDPAVIRAAVLEGTRDGAGEVREVGDRREAIRVALSSAGPGDIVLVAGKGHETGQLVGDRVLPFDDRQVAREAVR"
  },
  {
    "reference": "MYQKEHEEQIVSEILSYYPFHIDHVALKSNKSGRKIWEVETDHGPKLLKEAQMKPERMLFITQAHAHLQEKGLPIAPIHQTKNGGSCLGTDQVSYSLYDKVTGKEMIYYDAEQMKKVMSFAGHFHHASKGYVCTDESKKRSRLGKWHKLYRWKLQELEGNMQIAASYPDDVFSQTFLKHADKMLARGKEALRALDDSEYETWTKETLEHGGFCFQDFTLARLTEIEGEPFLKELHSITYDLPSRDLRILLNKVMVKLSVWDTDFMVALLAAYDAVYPLTEKQYEVLWIDLAFPHLFCAIGHKYYLKQKKTWSDEKYNWALQNMISVEESKDSFLDKLPELYKKIKAYREAN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Seems to be required for the assembly of the CotSA protein in spores..2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in endospore-forming forespore that is critical for sporulation resulting in formation of a cellular spore. The designed protein sequence is ",
    "response#1": "MWVYEKKLQYPVKVSTCNPTLAKYLIEQYGGADGELAAALRYLNQRYTIPDKVIGLLTDIGTEEFAHLEMIATMVYKLTKDATPEQLREAGLGDHYVNHDSALFYHNAAGVPFTASYIQAKGDPIADLYEDIAAEEKARATYQWLIDISDDPDLNDSLRFLREREIVHSMRFREAVEILT",
    "response#2": "MWVYEKKLQYPVKVSTCNPTLAKYLIEQYGGADGELAAALRYLNQRYTIPDKVIGLLTDIGTEEFAHLEMIATMVYKLTKDATPEQLREAGLGDHYVNHDSALFYHNAAGVPFTASYIQAKGDPIADLYEDIAAEEKARATYQWLIDISDDPDLNDSLRFLREREIVHSMRFREAVEILD",
    "response#3": "MWVYEKKLQYPVKVSTCNPTLAKYLIEQYGGADGELAAALRYLNQRYTIPDKVIGLLTDIGTEEFAHLEMIATMVYKLTKDATPEQLREAGLGDHYVNHDSALFYHNAAGVPFTASYIQAKGDPIADLYEDIAAEEKARATYQWLIDISDDPDLNDSLRFLREREIVHSMRFREAVEILK"
  },
  {
    "reference": "MLDTQLIDSLPYKLYRADQVRENERTAAEKAGVEMFTLMERAGKAVFHQSQLLLPNTDVYLVLVGQGNNAGDGYIAAINAKKAGKQVLLCAIEPHRILEGDAGVAQQKWLDAGGKIEPFNPGYLVKCGLVIDALLGTGINSYIRNEFADVIDAVNKSGKPVVSIDVPSGLDANTGQSLGRCVEADVTVTFVGIKPGLTTGAGKQSCGTLVFDDLGIGKTFVELARSCGSLLNIEQFKGLAPRDVHSHKGTYGRLLCIGGNRGTAGAIRLAGEAALRSGAGLVKVFCHEGSTVQVSAGRPELMVTCDNLHEALDWASCVVIGPGLGQDDWALAAFELAMKHCQTHPKPMVIDADALNLLSKHALSYSITDCILTPHAGEAARLLNMSVDEVEADRFNSVKQCAQRFHATCVLKGAGTIIDNETKTWVCQHGNPGMATAGMGDVLSGILGALLAQGLSKDIATKYGVSIHAKAGDEVARLYGERGMLASDLFESVRALINQ",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The (6S)-NADPHX binding site should be stable and able to withstand changes in environmental conditions.2. The designed protein must be able to perform ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, ATP binding, NADHX epimerase activity under specific conditions.3. Mg(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "MDQHKGQGGHTVVMGGSIPYLGAATMAGLGAVASGAALVTLSVHSSLAKEARMGYPGLMVASGNPEAPGWPRGKVASLVIGPGWGDEGLAYWFAVVMGDGPWCGLPTVFDADALNILAHDPGLRACDLPLNPSILTPHTGEAARLLAMSAEQVLENRVAAARVLAEKHRAVVVLKGPSTLIAAPDGRLAVNPTGHPLLAQGGTGDVLAGIIGGLQAQGMASFEAACCGTFLHGWAAERLAEHQGESGLRPELLG",
    "response#2": "MDQHKGQGGHTVVMGGSIPYLGAATMAGLGAVASGAALVTLSVHSSLAKEARMGYPGLMVASGNPEAPGWPRGKVASLVIGPGWGDEGLAYWFAVVMGDGPWCGLPTVFDADALNILAHDPGLRACDLPLNPSILTPHTGEAARLLAMSAEQVLENRVAAARVLAEKHRAVVVLKGPSTLIAAPDGRLAVNPTGHPLLAQGGTGDVLAGIIGGLQAQGMASFEAACCGTFLHGWAAERLAEHQGESGLRPELLA",
    "response#3": "IVVSGPPARTGAARLGARGALRIGAGLVTVASEPDALAANAAQLTAIMVEPFQGAVGLSDLLADERKNAALIGPGAGVGDETRENVLALLATQAALVLDADALTSFETDSDTLFAAIKARAATVAITPHEGEFQRLFSGAISEGSKLDRSRRAAEMSGAVVILKGADTVVAAPDGRAAINENAPPWLATAGSGDVLAGFITGLLAQHMPAFEAAAAAVWLHGAAAEAL"
  },
  {
    "reference": "MSFKDLRDFLNHLEKKGQLKRITHPVDPAYEMTEISDRTLRAGGPALLFENPIGYDIPVLTNLFGTAERVAMGMGREQVKELREVGQWLAYLKEPEPPKGFKDALDKLPVFKQVLNMPVKRLRKAACQEVVWQGEEVDLDKIPVMSCWQDDVAPLLTWGLTITRGPNKKRQNLGIYRQQKIAKNKIIMRWLAHRGGALDLRDWMEKYPGKPFPVSVAFGADPATILGAVTPVPDTLSEYAFAGLLRGSKTEVVKSASNDLEIPVSAEIVLEGYIDPNEYADEGPYGDHTGYYNEKEKHHVFTITHITMRKEPIYHSTYTGRPPDEPAVLGVALNEVFVPILQKQFPEIEDFYLPPEGCSYRMAVVTMKKQYPGHAKRVMMGVWSFLRQFMYTKYVVVCDESVNARDWDDVVKAMTENMNPILDSLFIESTPIDSLDFASPVAGLGSKMGLDATIKWEAELALQGATTSTEFASQIGFDVNALKAVHPLISDIYVPGVASGQFMVVKINKTHAGQCKEVVESLWQVESVKQRNKFIVVCDDDVKADDWHDIIWAITTRMDPARDTLKIDGSKSCSAKLVFDATNKYSEEITREWGKPIKKDPKLVAKVDALWQEFGIL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein can be expressed in plasma membrane and perform carboxy-lyase activity without triggering undesirable cellular responses.2. The protein must exhibit the following characteristics: Catalyzes the decarboxylation of 3-octaprenyl-4-hydroxy benzoate to 2-octaprenylphenol..3. The protein must be engineered to have a high affinity for a divalent metal cation in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "EETAPFHENEITEDINLFDILPLFRINQGDGGYYLDKACVISRDLEDPDNFGKQNVGIYRMQVKGKDRLGIQPVPQHDIAIHLRQAEERGINLPVTIALGCEPVITTAASTPLLYDQSEYEMAGAIQGEPYRIVKSKLSDLDVPWGAEVVLEGEIIAGEREYEGPFGEFTGHYSGGRSMPIIKIKRVYHRNNPIFEHLYLGMG",
    "response#2": "LFETFEEQELLDLVGGSRTVSYEPNEFILEFGEKASFLGILLEGEAELSVTLDNAERRIIDRISASDTFGEDAMMTGERNVADCICNKAARALLIPQGLFSETIITKPRALTHLSRMLG",
    "response#3": "EETAPFHENEITEDINLFDILPLFRINQGDGGYYLDKACVISRDLEDPDNFGKQNVGIYRMQVKGKDRLGIQPVPQHDIAIHLRQAEERGINLPVTIALGCEPVITTAASTPLLYDQSEYEMAGAIQGEPYRIVKSKLSDLDVPWGAEVVLEGEIIAGEREYEGPFGEFTGHYSGGRSMPIIKIKRVYHRNNPIFEHLYLGMGV"
  },
  {
    "reference": "MQTLAGKKILLGISGGIAAYKCAELTRRLIERGAQVQVVMTKAAKEFITPLTMQAVSGNPVSDSLLNPAAEASMGHIELAKWADIVLLAPATADLIARVSAGMGNDLLATLCLATDAPIAVAPAMNQQMYRNIATQENIATLARRGFEIWGPASGEQACGDVGPGRMLEPMQLVERIEHYFAPKALAGVKIAITAGPTCEAIDPVRYLTNHSSGKMGYAIAEAAAKQGADVTLISGPVNLATPAGVKRINVFSTKEMHQAALEQAVLHKIFIGCAAVADFRPAEVAEQKMKKQADSDEMVIRLVKNPDIIAAVAALEQQRPFTVGFAAETQNVEQYAMDKLSRKKLDLICANDVSAQNQGFNSDNNALILFWQTGQKESPLCSKAELGQQLIAEIAALYQQSTTSLS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.2. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.3. The protein should have a low Km value for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. A protein should be able to manipulate the activity of enzymes in the CoA from (R)- pantothenate: step 3/5 pathway, either by activating or inhibiting their function.5. The presence of Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.6. The designed protein must possess FMN binding, metal ion binding to support coenzyme A biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "MQIVLGVAGGIAAYKAVLLLRLLREAGHDVRVVPTRAALEFVGRPTWEALSGQSATPEVFDDVAHVPHVALGQHADLVIVAPATADLLARAAAGRADDLLTSTLLTARCPVLMAPAMHTEMWDHPATRANVATLRARGVHVLDPASGRLTGADTGPGRLPEPDQIAAAAELLLGRAGALPRDLAGRHVVISAGGTREPLDPVRYLGNRSSGRQGYALARTARDRGARVTVVAANVSLDDPAGVDVVEVETTDQLRTAVRAAARTADVVVMAAAVADFRPVAAAGHKVKKRSDGVVDPILLEANPDILAELTAERLRPGQVIVGFAAETGDEHTTVLEYGRAKARRKGADLLAVNAVGHGLAFGTTDNAVTILGADGSETPVPYGPKEALADVVWDLVVARLG",
    "response#2": "MQIVLGVAGGIAAYKAVLLLRLLREAGHDVRVVPTRAALEFVGRPTWEALSGQSATPEVFDDVAHVPHVALGQHADLVIVAPATADLLARAAAGRADDLLTSTLLTARCPVLMAPAMHTEMWDHPATRANVATLRARGVHVLDPASGRLTGADTGPGRLPEPDQIAAAATALLEGGAAQPGLAGTRVLVSAGGTREPLDPVRYLGNRSSGRQGWALARVAAARGADVTLVAANVEDTAPFGCRVVPVGSAGDLRSAMAEHAPAADVVVMAAAVADFRPTAVADTKMKKGSGSEPTAVALTRNADVLAELVAARPEGQLVVGFAAETGDADDDVLGHARAKLARKGCDLLVVNDVSAGQVFGRPDNAVTVELLGADGTRSPVPAGSKSALAHVVWDEVVRLRERS",
    "response#3": "MQIVLGVAGGIAAYKAVLLLRLLREAGHDVRVVPTRAALEFVGRPTWEALSGQSATPEVFDDVAHVPHVALGQHADLVIVAPATADLLARAAAGRADDLLTSTLLTARCPVLMAPAMHTEMWDHPATRANVATLRARGVHVLDPASGRLTGADTGPGRLPEPDQIAAAALELLEDSAAASLAGRHVVISAGGTREPLDPVRYLGNRSSGRQGYALARTARDRGARVTVVAANVSLDDPAGVDVVEVETTDQLRTAVRAAARTADVVVMAAAVADFRPVAAAGHKVKKRSDGVVDPILLEANPDILAELTAERLRPGQVIVGFAAETGDEHTTVLEYGRAKARRKGADLLAVNAVGHGLAFGTTDNAVTILGADGSETVIARGSKTEVARAVLDAVAARLA"
  },
  {
    "reference": "MDLLPFLLDANLSATNPPAIPHWWKRQPLIPNLLSQELKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPTGTYSLRQELIRTGD",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must be optimized for acyltransferase activity. The designed protein sequence is ",
    "response#1": "ELNTKPDDYDYVVFHQPNGKFPTRAAKILGFREEQYREGLITPYIGNTYSGSTMTGLSAILDVSRPGDRILAVSFGSGC",
    "response#2": "GKKPEDYDYAVFHQPNGKFPVRVAAKLGFTKEQIAPGLVVPHIGNTYSGSTLIGLAATLDQAEPGESIFVTSFGSGAGSDAFSR",
    "response#3": "MEKYGYKVEDFDYAVFHMPNAKFPVRAAKMLGFSMEHISQGLVVKAIGNTYSGSSLLGLAATLDVAEPDERILLVSFGSGAGSDAFAIL"
  },
  {
    "reference": "MTIDTAAWPAGRVNSLGFAKPPHETRVVAAMSGGVDSSVVAAMLKAEGYDVIGITLQLYDHGAAIEKKGACCAGQDIHDARNVSDAIGIPHYVLDYESRFREQVMEDFADTYLSGSTPIPCIRCNQTVKFADLLATAKELGADCLATGHYIRRTDGPEGPELHRAQDASRDQSYFLFATTRAQLDFLRFPLGSLPKSEVRELAEKFALQVAAKPDSQDICFVPDGSYAKVVEKLRPGSGRGGEIVHLDGRVLGKHEGVIHYTIGQRRGLGVATGDPLFVVKIDAPARRVIVGPREALMTRGLLLEELNWLGQGSLEEAATHGARVLIRVRSTRPPVPGRLGYEDGVPAVFFDAPEEGVARGQAAVLYDLEGSTRILGGGFIARPLPADERVVAA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a binding site that is specific to the target ATP ligand.2. A protein that can perform tRNA binding in cytoplasm to support tRNA modification.3. The protein with the reaction AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in   tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-   cysteinyl-[protein], the Cysteine persulfide intermediate must be optimized to ensure efficient catalysis.4. The protein must exhibit the following characteristics: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34.. The designed protein sequence is ",
    "response#1": "MDRIVLRDVNWIGDSSLDRAVGDGLELFVRVRSTRRPQPAWLRAQDGGYQVELVAGEDGVSPGQACVFYDAPAGQARVLGGGFISR",
    "response#2": "MDRIVLRDVNWIGDSSLDRAVGDGLELFVRVRSTRRPQPAWLRAQDGGYQVELVAGEDGVSPGQACVFYDAPAGQARVLGGGFL",
    "response#3": "MSEGLRAIKVNWILRRESDEPFECTAKFRYRQPDQKVTVYPQSDGAVEVLFAEPQRAITPGQAVVFYDGDVCLGGGTIV"
  },
  {
    "reference": "MEQSASSCMRSPHTGGALWGCVRSPHSGGSGAGIQPYQQAPFALHQKHDFLAYTDFSSSCLVPAPHAYPREDRLYPETHSGYQRTEWQFSPCEPRGRGQEPCQGAAEAVGAEMDSAGGDRLAGAVTGCLEGDYSPQSVPAVDTEKKSSKRKREVTDIQDSSFKADSNCKARKERTAFTKEQLRELEAEFTHHNYLTRLRRYEIAVNLDLTERQVKVWFQNRRMKWKRVKGGQPASPHDLEADELDSAASPSSE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The Homeobox domain within the designed protein should exhibit high affinity and specificity towards its target DNA sequence.2. The designed protein should be involved in muscle cell development, sclerotome development, hematopoietic stem cell differentiation by localizing to nucleus and exhibiting RNA polymerase II cis-regulatory region sequence-specific DNA binding, sequence-specific DNA binding.3. The protein must exhibit the following characteristics: Required for hematopoietic stem cell (HSCs) induction via its role in somitogenesis: specification of HSCs occurs via the deployment of a specific endothelial precursor population, which arises within a sub-compartment of the somite named endotome; Required for maintenance of the sclerotome polarity and formation of the cranio-cervical joints; Acts by mediating specification of endothelial cells of the endotome within the nascent somite, notably by repressing expression of cxcl12b.; Binds specifically to the promoter of target genes and regulates their expression; Mesodermal transcription factor that plays a key role in somitogenesis and is specifically required for sclerotome development. The designed protein sequence is ",
    "response#1": "MRRPRTAFSSEQLVQLEKQFSDNRYLSRPRRYQLAQQLSLSETQIKIWFQNRRMKNKRCPSSNMRRPRTAFSSEQLVQLEKQFSDNRYLSRPRRYQLAQQLSLSETQIKIWFQNRRMKNKRCPSSKR",
    "response#2": "GRKQDIGDILQQIMTITDQSLDEAQARKHALNCHRMKPALFNVLCEIKEKTELKEARSRNCPSLFFLVTRIQSSVRHRVLSSASTSGNPVTPQPTHHHPSFGWGEGKVLSIRGAQEEEPTDPQLMRLDNMLLAEGVAGPEKGGGSAAAAAAAAASGGAGSDNSVEHSDYRAKLSQIRQIYHTELEKYEQACNEFTTHVMNLLREQSRTRPISPKEIERMVSIIHRKFSSIQMQLKQSTCEAVMILRSRFLGRKQDIGDILQQIMTITDQSLDEAQARKHALNCHRMKPALFNVLCEIKEKTELKEARSRNCPSLFFLVTRIQSSVRHRVLSSASTSGNPVTPQPTHHHPSFGWGEGKVLSIRGAQEEEPTDPQLMRLDNMLLAEGVAGPEKGGGSAAAAAAAAASGGAGSDNSVEHSDYRAKLSQIRQIYHTELEKYEQACNEFTTHVMNLLREQSRTRPISPKEIERMVSIIHRKFSSIQMQLKQSTCEAVMILRSRFLE",
    "response#3": "MKARALVAFHQGIYAELYSILESHNFDSSNHPLLQELWYKARYTEAERARGKPLGAVDKYRLRRKYPLPEGRKQDIGDILQQIMTITDQSLDEAQARKHALNCHRMKPALFNVLCEIKEKTELKEARSRNCPSLFFLVTRIQSSVRHRVLSSASTSGNPVTPQPTHHHPSFGWGEGKVLSIRGAQEEEPTDPQLMRLDNMLLAEGVAGPEKGGGSAAAAAAAAASGGAGSDNSVEHSDYRAKLSQIRQIYHTELEKYEQACNEFTTHVMNLLREQSRTRPISPKEIERMVSIIHRKFSSIQMQLKQSTCEAVMILRSRFLDINALQKDLEQFAKLLKQKRITLGYTQADVGLTLGAIFGKVFSQTTICRFEALQLSFKNMCKLRPLLQRWVEEAGDIGDILQQIMTITDQSLDEAQAKKHALNCHRMKPALFSVLCEIKEKTGLSMRNVQEEEPPDPQLVRLDNMLLAEGVAGPEKGGGAAAAVSAATSSGGMSPDSSLEHSDYKSKLSQIRNIYHTELEKYEQACSEFTTHVMNLLREQSRTRPVSPREIERMVAIIHRKFSSIQTQLKQSTCEAVMILRSRFLDINALQKDLEQFAKLLKQKRITLGYTQADVGLTLGAIFGKVFSQTTICRFEALQLSFKNMCKLRPLLQRWVEEAGGRKQDIGDILQQIMTITDQSLDEAQARKHALNCHRMKPALFNVLCEIKEKTELKEARSRNCPSLFFLVTRIQSSVRHRVLSSASTSGNPVTPQPTHHHPSFGWGEGKVLSIRGAQEEEPTDPQLMRLDNMLLAEGVAGPEKGGGSAAAAAAAAASGGAGSDNSVEHSDYRAKLSQIRQIYHTELEKYEQACNEFTTHVMNLLREQSRTRPISPKEIERMVSIIHRKFSSIQMQLKQSTCEAVMILRSRFL"
  },
  {
    "reference": "MTVNALLLSSSRVGDTPYLSHAIPFIKPLTTNAQKWIFIPYAGVSMSYDTYLASVVAGLSELRLDISGIHQHPDPRQAIKDADGILIGGGNTFHLLHELYKYDLVHLIREEVMNGKPYIGWSAGSNVSGLSIRTTNDMPIIEPPSFTALNIVPFQLNPHYSNYRAPGHNGETRAQRLLEFTRVDPITPVVGIVEGSALWRQGETLSLLGDNPAYLFCGEQQEIPIPVGSDLSHLLK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should be able to enhance the efficiency of proteolysis.2. The design of the protein must focus on the precise arrangement of amino acids in the Charge relay system to enable effective catalysis of Dipeptidase E catalyzes the hydrolysis of dipeptides Asp-|-   Xaa. It does not act on peptides with N-terminal Glu, Asn or Gln, nor   does it cleave isoaspartyl peptides.. The designed protein sequence is ",
    "response#1": "NAKELLPDILVQNNTKESTWETYSSWQSKNFTKLYLNSDSLSAQKKENQTLEFSDHLPETTFKHYQTDIANWKEEILASTSPKLETNRLILTSKPLKHETLLKGVAKIKLKIASQLDHGLVSVKLVDYGDAKRLGATPTILERRGLDLGYHWKEDNLVEFKLAKETPFKMITQAHLNLQNRHNDFSTDELEANKFYDVEITTQPMFYHLPKGHKLGLK",
    "response#2": "DGKRDLLKVIITRPAMTDNGMKVPTIFTASPYYLGTNDASAEKMMHSVDLPITRKEVKPLSYQDIEYHKPETKLPKKRPVVISTKNAEESWEHLFTYTFNDYMLARGFAVVYSGGVGTLDSDGYRTCGDEAETLGAKDVVEWLNGKRTAFTTKEANKAIPAWWSNGKVAMTGKSYLGTLATAAATTGVAGLETIISEAAISSWYDYYREGGLVIAPGGFPGEDADILAEECFSRQKSAGDYNHSKDGFNKFLSTITKDQDRTTGNYNTFWDARNYLKDVGNIKCDIVMVHGLNDWNVKLKNVFNLYNKLGDVEVTKKLILHQGQHIYALNGGEHGMDYFTNLWLSHKLYGVENNAKELLPDILVQNNTKESTWETYSSWQSKNFTKLYLNSDSLSAQKKENQTLEFSDHLPETTFKHYQTDIANWKEEILASTSPKLETNRLILTSKPLKHETLLKGVAKIKLKIASQLDHGLVSVKLVDYGDAKRLGATPTILERRGLDLGYHWKEDNLVEFKLAKETPFKMITQAHLNLQNRHNDFSTDELEANKFYDVEITTQPMFYHLPKGHKLGLP",
    "response#3": "DGKRDLLKVIITRPAMTDNGMKVPTIFTASPYYLGTNDASAEKMMHSVDLPITRKEVKPLSYQDIEYHKPETKLPKKRPVVISTKNAEESWEHLFTYTFNDYMLARGFAVVYSGGVGTLDSDGYRTCGDEAETLGAKDVVEWLNGKRTAFTTKEANKAIPAWWSNGKVAMTGKSYLGTLATAAATTGVAGLETIISEAAISSWYDYYREGGLVIAPGGFPGEDADILAEECFSRQKSAGDYNHSKDGFNKFLSTITKDQDRTTGNYNTFWDARNYLKDVGNIKCDIVMVHGLNDWNVKLKNVFNLYNKLGDVEVTKKLILHQGQHIYELDDVKAAFEVG"
  },
  {
    "reference": "MKYIFITGGVVSSLGKGLAAASLGTLLELRGLRVVFQKFDPYLNVDPGTMNPFQHGEVYVLNDGAETDLDLGHYERFTNCILSRHNNLTSGQVYESVILKERRGDYLGKTVQVIPHVTDVIKDRIRELSDASKYDVVITEIGGTTGDIEGLPFLEAIRQFILEAGPQNVVNMHVTLVPYIKAAHELKTKPTQQSVAKLREIGLQPQVLICRTEKPLDRDVRQKLSMFCSVSEKAVIDARDVKHTIYEYPLMLHAEGLDQLVCDLLQLKTSQPDLADWRKFVERVVSPKKRIKIAVVGKYIDLQDAYKSIYESLTHAAASQDCGIDLKLIDAESLEDGVDGQLKDVAGILIPGGFGVRGVEGKINAARFAREHKVPYLGLCLGMQVATIEFARNVCGIEGANSTEFDRNAAEPVISLLEEQRGIRNKGGSMRLGTWPTKIVEGTLARKIYGHDEVMERHRHRYEFNMRYRDCMAEKGFTISGTSPDGTLAELIELRDHPYFLACQYHPEFQSKPNKPHPLFRGFIQACLAYPAGHAPRVAADPLSPPRVPEEELVPQR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a binding site that is specific to the target Mg(2+), ATP, UTP, CTP, L-glutamine ligand.2. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen3. A protein should be able to manipulate the activity of enzymes in the Pyrimidine metabolism, CTP biosynthesis via de novo pathway, CTP from UDP: step 2/2 pathway, either by activating or inhibiting their function.4. A protein with ATP binding, metal ion binding, glutaminase activity is needed to enhance 'de novo' CTP biosynthetic process, glutamine metabolic process in a specific context.5. The designed protein must have a Nucleophile, Nucleophile; for glutamine hydrolysis that is structurally stable and resistant to denaturation.6. The designed protein have CTP synthase N-terminal, Glutamine amidotransferase domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "YVFVTGGVVSGLGKGVTTASLGALLKARGLKVDVVKMDPYINVDPGTMSPNQHGEVFVTADGAETDLDLGHYERFIQNPMGRANNFTTGSVYDTVLKRERAGDYDGVTVQVIPHITDEIKARIRAVSKDNDVVIVETGGTVGDIESQPFLEAIRQMRLEEGTRNTLFIHLTLVPQLSDGEDKTKPTQHSVKELRSIGLQPDILICRCKGQELDAGPRKKIALFTNVPEDAVISLANVESIYQVPLVLNQQRMDEIVLDSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASFVDSYKSLTEALAHGGFQHGLKVNINWVEAEALEMDGGERLLDDAHGILVPGGFGDRGSRGMMEAARIARERRIPYFGICYGFQWAAVEFARNVCGLEDADSTECREDAPHKVIYKLRDLLGVDELGGTMRLGSYPCRLEPGSLAARLYGTSLVDERHRHRYEFNVEYQQRLTDRGLSIAGRSPDGKFVEVIELPQHPWFLAVQFHPEFQSKPLRPHPLFADFVRPTAAQDSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASFV",
    "response#2": "KFVFVTGGVVSSIGKGIVAASLGRLLKSRDYSVSILKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTDTSMSRLNSVTTGSIYQAVINKERRGEYNGRTVQVIPHVTNEIKERIRRVAQNRNPDVVIVEIGGTVGDIEGLPFLEAIRQFRKDVGRKNVIYMHVTLIPYIPAAGEIKTKPTQHSVKELRSIGLQPDILVCRCAQPIPEHIKAKVSDFCDVPEACVIAANDAPSIYEVPLLMEQEGLARLLNLAGSRPDLSDWDRMVQRLLSPKHRITIGLVGKYVELKDAYKSISEAIIHAGAKNSCKVNIEWLHAEKINTNNVKQKLSKLKGIIVAPGFGDRGIEGKITAIKYIRENNIPFFGICLGMQSAVIEYARNVLGLRNANSTEMDSKTSXPVIDLMNEQKEVTKKGGTMRLGTYDCYLKNNSNAYNAYKNNNIKERHRHRFEFNNKYKNDLEKAGLIATGINKNKNLVEIIEIAQHPWFVGVQFHPEFKSTVDNPHPLFCGFIRACVERSRL",
    "response#3": "KFVFVTGGVVSSIGKGIVAASLGRLLKSRDYSVSILKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTDTSMSRLNSVTTGSIYQAVINKERRGEYNGRTVQVIPHVTNEIKERIRRVAQNRNPDVVIVEIGGTVGDIEGLPFLEAIRQFRKDVGRKNVIYMHVTLIPYIPAAGEIKTKPTQHSVKELRSIGLQPDILVCRCAQPIPEHIKAKVSDFCDVPEACVIAANDAPSIYEVPLLMEQEGLALPDAYLSVVEAVRAAGFHHHIRAEIAWIASDDCDTPEGAAAALSTVDAVVIPGGFGNRGIEGKIGAVTHCRENNIPLLGICLGLQCIVIEAARAAGVLAATSTEFDPDTAEPVISTMAEQQAAVSGEADLGGTMRLGSYPARLAEGSLLVDLYGSTEVNERHRHRYEVNNTYRETIEAGSGLEFSGTSPDGELVEFVEYSTDLHPYLVGTQAHPEYKSRPTKAHPLFIGFLAAMQG"
  },
  {
    "reference": "MPKTKHWHYSIPPEELDDERTAKAFIRELRISPKHAREICRAIRGMPLDRAKEFLRRVIRKEEAVPFRKHKKKVPHRRQIRPGWDAGRFPEKAAREILRVLEHAEANAEYKGLDTDRLYIKHIAAHKGRVIRGWIPRAFGRATPFNTPTTHIEVILEER",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome. The designed protein sequence is ",
    "response#1": "GKRFRPRARGRAARIRKRLSHITVVS",
    "response#2": "GKRIMPRAKGRADRILKRGCHITVKL",
    "response#3": "MPK"
  },
  {
    "reference": "MKTNGAMTGRGTLKGSPGDKYHRTWAQYFIRFLDEYARHNLTFWAVTAGNEPTAGEIVFYPFQCLGFSPEHQRDFIARDLGPALANSSHRHVQLIILDDQRVMLPYWGRTPGLHPCCRCLLFLH",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must have at least one Glycosyl hydrolase family 30 TIM-barrel domain.2. The protein have enhanced cholesteryl 3-beta-D-glucoside + H2O = cholesterol + D-   glucose.3. A protein that is capable of catalyzing reactions within the cholesterol metabolism, Steroid metabolism pathway with high efficiency and specificity.4. The ideal protein would be one that plays a critical role in sphingolipid metabolic process, cholesterol metabolic process through glucosylceramidase activity, glycosyltransferase activity within lysosomal membrane. The designed protein sequence is ",
    "response#1": "MPMASCDFSVRPYTYDDFPYDYELKNFSLVGEDLLMKIPIVHRAKAMSKRPISLMASPWTAPIWMKSNEAWKGKGWLKGEAGDSIHKTWASYFVRFLDEYAKQNLTFWAITAQNEPSSGFVKDYPFQTLGFTAESQREFIASDLGPALAKSSHKNIRLITLDDNRLHLPKWAKEVLSTESQAYRYIHGIGLHWYMDFISPIKGTVEATQRLFPDYFLIATEACAGSFFWEQDVILGSWDRGEDYSHSILENLNHHVVGWVDWNLALDLQGGPNWVKNYVDSPIIVDASSDIFYKQPMFYHLGHFSKFL",
    "response#2": "MPMASCDFSVRPYTYDDFPYDYELKNFSLVGEDLLMKIPIVHRAKAMSKRPISLMASPWTAPIWMKSNEAWKGKGWLKGEAGDSIHKTWASYFVRFLDEYAKQNLTFWAITAQNEPSSGFVKDYPFQTLGFTAESQREFIASDLGPALAKSSHKNIRLITLDDNRLHLPKWAKEVLSTESQAYRYIHGIGLHWYMDFISPIKGTVEATQRLFPDYFLIATEACAGSFFWEQDVILGSWDRGEDYSHSILENLNHHVVGWVDWNLALDLQGGPNWVKNYVDSPIIVDASSDIFYKQPMFYHLGHFSKFIPEGSQRVGLVASKESKKVDLEYTAFLRPDGAVVVVVLNK",
    "response#3": "MPMASCDFSVRPYTYDDFPYDYELKNFSLVGEDLLMKIPIVHRAKAMSKRPISLMASPWTAPIWMKSNEAWKGKGWLKGEAGDSIHKTWASYFVRFLDEYAKQNLTFWAITAQNEPSSGFVKDYPFQTLGFTAESQREFIASDLGPALAKSSHKNIRLITLDDNRLHLPKWAKEVLSTESQAYRYIHGIGLHWYMDFISPIKGTVEATQRLFPDYFLIATEACAGSFFWEQDVILGSWDRGEDYSHSILENLNHHVVGWVDWNLALDLQGGPNWVKNYVDSPIIVDASSDIFYKQPMFYHLGHFSKFIPEGSQRVGLVASKESKKVDLEYTAFLRPDGAVVVVVLNR"
  },
  {
    "reference": "MNTAIILAAGQGTRMKSQTAKVLHKVGNKCLLQHIVDASSDHVETINVIVGHNSQAVKAAMPKNKINWVLQEKQLGTGHAVQQAIPFINDDSICLILYGDVPLLQSKTLEELLIKARPSGFSLLSVVLNDPFGYGRIIRDSNGLIQSIVEQKDASESELKVKEINTGIMAILGSLLKKYLNELKPNNVQGELYLTDIVESAVKDNVNIASFVCENASEVMGINDKKQLSQAERSYQVRQAEDLMSSGLTIMDPRRFDCRGDLNFGDDCTIDVNVVFEGDNVLGKNVTISPNCIIKDSKIGDNTSILPNSIIENSIIGSSTTIGPFARIRPDTTIGDHSKVGNFVEVKKSTIKSNSKVSHLSYIGDSNIGADVNIGAGVITCNYDGSNKHQTEVKDGAFIGSNSQLVAPVIIGKNATIGAGSTITQNAPDDKLTLSRNKQSTIDKWKRPTKK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.2. The designed protein should have a high specificity for acetyl-CoA binding, with minimal off-target effects.3. The protein must have high specificity towards magnesium ion binding.4. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc) The designed protein sequence is ",
    "response#1": "MAGGFGTRMRSELPKHLHPLLGRRIIDWILEAVRGLEPNPLVVVASPATKDRFEGVEVAVQAVPRGTGDALASARDLLAGRARSLLVLSGDTPLLTSELLGELVAAHRRSDARATLSRP",
    "response#2": "MAGGFGTRMRSELPKHLHPLLGRRIIDWILEAVRGLEPNPLVVVASPATKDRFEGVEVAVQAVPRGTGDALASARDLLAGRARSLLVLSGDTPLLTSELLGELVAAHRRSDARATLL",
    "response#3": "MAGGFGTRMRSELPKHLHPLLGRRIIDWILEAVRGLEPNPLVVVASPATKDRFEGVEVAVQAVPRGTGDALASARDLLAGRARSLLVLSGDTPLLTSELLGELVAAHRRSDARATLWADR"
  },
  {
    "reference": "MAKQNTVLIKLVSSADTGFFYVKKKNPRKTTEKLEFKKYDPVARKHVVFKEAKMK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be able to modulate translation in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "MKTNGKFRYNFHRRDWRG",
    "response#2": "MPIGVPKVPFRLPGQ",
    "response#3": "MF"
  },
  {
    "reference": "MASIILESIFLKRSQQKKKTSPLNFKKRLFLLTESKLSYYEYDFERGRRGSKKGSVDIEKITCVETVVPENNPPPERQVPKKGEDYNMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKSVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCKILESRNGSLKAGRSHRKTKKPLPPTPEEDTMVMKPLPPEPAPSAAGEMKKVVALYNYVPMNVQDLQLQKGEDYLILEESHLPWWKARDKNGREGYIPSNYVTATSNSLEIYEWYSKNITRSQAEQLLKQEGKEGGFIVRDSTSKTGKYTVSVYAKSAVDPQGMIRHYVVCCTPQNQYYLAEKHLFNTIPELITYHQHNSAGLISRLKYPVSRHQKSAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRGQYNVAIKMIREGSMSEDEFIDEAKVMMNLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNFLRETQRRFQPAELLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGIVKVSDFGLSRYVLDDEYTSSMGSKFPVRWSPPEVLLYSKFSSKSDVWSFGVLMWEVYSLGKMPYERFNNSETTEHVIQGLRLYRPQQASERVYAIMYSCWHEKAEERPTFSALLGSIVDITDEEP",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must bind to Zn(2+) in order to perform its enzymatic function.2. The designed protein should contain at least one CAV1-binding motif to achieve its intended function.3. The designed protein must be able to regulate positive regulation of NLRP3 inflammasome complex assembly.4. The protein must exhibit the following characteristics: BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF- kappa-B ; BTK has a dual role in the regulation of apoptosis .; ARID3A and NFAT are other transcriptional target of BTK ; There is however no evidence that BTK itself binds directly to DNA .5. The presence of SH2 domains is critical for the interaction of the designed protein with its intended targets.6. The designed protein should be able to compete with endogenous proteins or inhibitors for 1D-myo-inositol 1,3,4,5-tetrakisphosphate binding. The designed protein sequence is ",
    "response#1": "MNNPLSHYWISSSHNTYLTGDQLRSESSPEAYIRCLRMGCRCIELDCWDGPDGKPVIYHGWTRTTKIKFDDVVQAIKDHAFVTSSFPVILSIEEHCSVEQQRHMAKAFKEVFGDLLLMKPTEASADQLPSPSQLREKIIIKHKPTVKVTQQSPTSMNQVNLTCRAERFYPEDLQLIWLENGNVSRNDTPKNLTKNTDGTYNYTSLFLVNSSAHREDVVFTCQVKHDQQPAIREMKTFLEKLCFELDYEPAHISLDPQTSHPKLLLSEDNQQARFSYKWQNSPDNPQRFDRATCVLAHSGFTEGRHTWVVSVDLAHGGSCTVGVVSQDIRRKGELRMRPEEGVWAVRLAWGFVSALGSFPTRLALEEHPRQVRVSIDYEVGWVTFVNAVTQEPIYTFTASFTQKVFPFFGLWGRGSKFSLSD",
    "response#2": "MNNPLSHYWISSSHNTYLTGDQLRSESSPEAYIRCLRMGCRCIELDCWDGPDGKPVIYHGWTRTTKIKFDDVVQAIKDHAFVTSSFPVILSIEEHCSVEQQRHMAKAFKEVFGDLLLMKPTEASADQLPSPSQLREKIIIKHKD",
    "response#3": "MNNPLSHYWISSSHNTYLTGDQLRSESSPEAYIRCLRMGCRCIELDCWDGPDGKPVIYHGWTRTTKIKFDDVVQAIKDHAFVTSSFPVILSIEEHCSVEQQRHMAKAFKEVFGDLLLMKPTEASADQLPSPSQLREKIIIKHKR"
  },
  {
    "reference": "MRGLLQDIPVRGQKRVLLAAPRGYCAGVDRAVIAVEKALERYGAPVYVRKQIVHNIHVVSELERQGAIFVDEVDEVPEGAHIVFSAHGVSPAVVAAAADRGLRAIDATCPLVTKVHREAVRFARDDFEILLIGHEGHEEVEGTAGHAPERVTLVNSPDEADTIEVKDPDRVVWLSQTTLSVDETMETVRRLRERFPNLQNPPSDDICYATQNRQVAIKKVAQSADLVIVVGSANSSNSVRLVEVALEYGAKAAYRVDYASEIRQEWLDGVATVGVTSGASVPEVLVQEVLADLAGAGYADVQEVKTAEEDLMFSLPKELRKDIAGKRDERALGGRRS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway, terpenoid biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.2. The protein should have a high affinity for substrate.3. The protein design should consider the endogenous proteins and enzymes present in the isopentenyl diphosphate biosynthesis via DXP pathway pathway to avoid interference with native metabolic processes.4. The Proton donor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "ITHPEDFARAERILAGDNAMETRVGTGFDVHPFEPGDAVWLGGVKIAHDKKLKGHSDADVALHALTDAILGAIGEGDIGVHFPPSDMQWKGAASTVFLAHAGKLVLEAGGRIVNLDVTIVCEAPRIAAHVPAMRDVIGTVLGIATSRIAVKATTSEQLGFTGREEGIVAMASASVY",
    "response#2": "IRTGNGYDVHSFEEGTFLTLCGIKIPFHKKLNGHSDADVALHALTDALLATQGAGDIGTHFPPSDPQWKNESSEIFLRHALDIVKQAGGRIANVDITLIAEEPKIGPYRQTMRENLMRILTISSDRISIKATTNEKLGFIGRGEGIAAFATASVLVE",
    "response#3": "RVGTGFDVHPFEPGDAVWLGGVKIAHDKKLKGHSDADVALHALTDAILGAIGEGDIGVHFPPSDMQWKGAASTVFLAHAGKLVLEAGGRIVNLDVTIVCEAPRIAAHVPAMRDVIGTVLGIATSRIAVKATTSEQLGFTGREEGIVAMASASG"
  },
  {
    "reference": "MSNKDQTNLLNNLKDIAQKAGLDISEELAKINAKLESSTALSKTWERVELARHSDRPRTLDYINLIFDNFTELHGDRFFGDDPAMIGGIGFIDGMPVTVIGTQKGRNLRETIDRNGGMANPEGYRKAMRLAKQAEKFKRPIITFIDTQGAYPGLGAEERGIGEAIAFNLREFSRLKTPIICIIIGEGGSGGALGIGVGDKIYMLENAIFSVISPEGCASILLRDSSRAKDAAAMLKITSQEVLDLKVINGIIPEPEKGAHTDPKKTADAIKEQILKDLADLTKRDPAVLVKYRSKKIRSIGKYSE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should interact with enzymes and substrates within the malonyl-CoA biosynthesis, Lipid metabolism, malonyl-CoA from acetyl-CoA: step 1/1 pathway to enhance or regulate its activity.2. The designed protein have CoA carboxyltransferase C-terminal domains with specific sequence motifs that are crucial for its activity.3. The protein should have a low Km value for acetyl-CoA + N(6)-carboxybiotinyl-L-lysyl-[protein] = malonyl-   CoA + N(6)-biotinyl-L-lysyl-[protein].4. The designed protein should be optimized for ATP binding, acetyl-CoA carboxylase activity activity in order to improve fatty acid biosynthetic process. The designed protein sequence is ",
    "response#1": "LWVKCPVSEEMVFHKDLEAGLFVTPAGHHMRIAPAYRFQFTFDGDYTEIETPEVVKDPLKFRDDKPYTAKLAAARKKTGRDDSMAIATGTIGGLDAVVLVQNFAFMGGSLGMAAGESFIKAAETALARKAPMIVFTAAGGARMQEGALSLMQMPRTTLAVEMLREAGLPYIVVLTDPTTGGVTASYAMLGDVHIAEPGALIGFAGPRVIEQTIREKLPEGFQRAEYLLEKGMVDKVVHRADIPATLARMLRVLMKKPGTSMPAALTPG",
    "response#2": "LEERAKQLRYEIFSKLTPGQTLQVARHPRRPSTLDYIQAISEEWIELHGDRRGSDDPAIVGGIGRLNDQPVVMLGQQKGRDTKDNVARNFGMASPGGYRKALRLMEHANRFQMPILTFIDTPAAWAGVDAEKFGQGEAIAYNLREMFRFEVPIICTVIGEGGSGGALAIGVGDRLLMFEHAVYSVAPPEACAAILWRDAQKAPQAAEALKITARDLLKLGIIDEIVPEPVGAAHSNPVEAAENLKAALLRNLAELLHEA",
    "response#3": "LEERAKQLRYEIFSKLTPGQTLQVARHPRRPSTLDYIQAISEEWIELHGDRRGSDDPAIVGGIGRLNDQPVVMLGQQKGRDTKDNVARNFGMASPGGYRKALRLMEHANRFQMPILTFIDTPAAWAGVDAEKFGQGEAIAYNLREMFRFEVPIICTVIGEGGSGGALAIGVGDRLLMFEHAVYSVAPPEACAAILWRDAQKAPQAAEALKITARDLLKLGIIDEIVPEPVGAAHSNPVEAAENLKAALLRNLAGRLTGPGGPLPAALTVGESWDRVRAARRLGRPTTLDYLAGAFDDFVELHGDRMSTDCPAMIAGLARLDGLPVVVLGTQKGHTGPELTERNYGMPTPAGYRKAVRVLRLAAKLGRPVVTLVDTAGAYPGIEAEKQGQAVAIAESLKLLAGLPVPVVTVITGEGGSGGALALALADRVLVCENAIYSVISPEGCAAILWKDPAAAPRAAAALRVDAAALLELGIADAVVPEPAGGADRDPVGAAALLRRAVRRAVHELALRFG"
  },
  {
    "reference": "MLRNGAQNGNINSESHESFGKAAKGFRIFSSFSSSQKLFQRRSSGSITHSPTALSSTTSLNENDGNHFRPASSLSFSPSSLSRKDSGPGDGLEVNKKNNFYRRSSSTDDFGISHARSRKEIQSLGRPHTRQSFSVSDVSNGSSYPNIRKNSVHVNAPMPSFPEGSTAVLLKHHSGSKSASAISNIAPSHSNSTSSRRPYIHPAFLSQVAVEFRKRLNIGDRVKDGLLYKDAFLGSEAVDVLMHIVRTTDRNLALLLGRALDSQKMFHDVTYSHRLRDSLKEVYQYRRIISPPPGLSSMDSNGSSIENNFLYTKRRANTSDSFDSVLSDSSTTPTISSSVQVNSLAFITSSLSAITKEPEAPETEYNPHGVFTLLTECYSSTCSRNRLCYSISCPRRLEQQARLHLKVQPVLSGGSTSITDKQEEDHRLWSENVPKQVVDQIDVREWKRQEIIFEVIYTERDFVRDLEYIRDFWIKPLSTSNVIPENNRQQFIRCVFHNIMQIHAVNSRLSNALNRTQTLQPVVNTIGDLFLDYVPKFEPFIKYGANQAIAKFEFEREKSTNRNFANYVHEVERLRESRKLELNGYLTKPTTRLARYPLLLSGVLKYTDKDNPDTENIPRVIEMIREFLTKLNYETGKTENRLSLLQLNEQLSCSPADRAKLTLFDPSRLLIFKGVVKLKASSYSNGDTENDIHMFLLDNFLLLCKIKIQMKRRVHKLHLRPLPLELLSISYIEDSPSRGSLPRRPSSALLTNPISITKSNPPPVKAYG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must be able to regulate division septum assembly.2. For general function, the protein need meet that Stimulates the exchange of Rho1 and Rho5 GDP-bound form into GTP-bound form The designed protein sequence is ",
    "response#1": "TLIPTIVVKLLEEIELRGLDEVGLYRIPGSIGSINALKNAFDEEGATDNSFTLEDDRWFEVNAIAGCFKMYLRELPDSLFSHAMVNDFTDLAIKYKAHAMVNEEYKRMMNELLQKLPTCYYQTLKRIVFHLNKVHQHVVNNKMDASNLAIVFSMSFINQEDLANSMGSRLGAVQTILQDFIKNPNC",
    "response#2": "VPLEDVCERENTLIPTIVVKLLEEIELRGLDEVGLYRIPGSIGSINALKNAFDEEGATDNSFTLEDDRWFEVNAIAGCFKMYLRELPDSLFSHAMVNDFTDLAIKYKAHAMVNEEYKRMMNELLQKLPTCYYQTLKRIVFHLNKVHQHVVNNKMDASNLAIVFSMSFINQEDLANSMGSRLGAVQTILQDFIKNPNDYL",
    "response#3": "TTRILCCEDVKFEGRIYLFVATTDGIYVKYRDDYGSGFVKILELNDVKRMEANVKLGLLFVLDNRKLCYFNISTVVSRYLAQGNTLDENCIVGTVIRDKVRFFKIADDFGNSKHLFFERKGKIVILTPEFDQLTNQVKYFKFYKEYKLPSSSNNILNNEIEDIAIFRKSFAVCTKKTVILYQDSFEDNGIVLPSFLNDKDMMAHLRHPHLNSLPFKSATDSKKRPSIESLTEEAKKDIATCKAIPVNFFQISQSSFFALVYDEAVVKINCYGEMSDWRKDILLLDFCCTGASFHGNHLILVGDNLIQIYDLKNVEQNLGELVPVQIIKGKKIKLASSERREA"
  },
  {
    "reference": "MSGGDGGSRAGTISYALPSIPSSKLPDLRFISRGAYGTVSAARHADWRVPVALKCLQGPLLDSDRNHLLKEAEILHKARFRYILPILGICNESEFLGIVTEYMTNGSLNQLLHGKDVYPDVAWCLRFRILYEIALGVNYLHNMNPPLLHHDLKTQNILLDDEFHVKIADFGMSKWRVISMSQSRSETSLPEGGTIIYMPPEDYNPSQKTRASVKHDIYSFAIIMWEVMSRKQPFEEVINPLQIMYSVSQGQRLDLSEESLSVDVPHRALIIRLIESGWAQNPDERPSFLKCLIDLEPVLRTFEEIDMLEAVILLKRSKSLYESRCLYNSEKKADVEQISLNIPLNSQEETYSASIQCDALPLRSISSDISRLESVPKCNILSSGRDTHDFIVLFEHRSNTTLFFFYFFFKDGNSGGVDPLSSLSTDENISVSQSAKLLVHPHRYFPSSDKNISGASNAASCVLRSSPTPSDFVLETIQQNVAHQWIQSKREEIIDQMTEACLNQSLDALLSRFLLMKEDYELISTKPTRTSKVRQLLDTSDSQGEEFARIIIQKLKDNKQLGLQPYPDVVSNSLRLHL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be able to bind ATP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MHVRSTIDVPPDLVVGLLGSADENLRALERMLSADLHVRGNTVAFSGEPADVALAERAISELVS",
    "response#2": "PWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPSTIFIDEVDSLCSTRGSDSEHEASRRFKAELLIQMDGLNNASTGEDKSILVLAATNHPWDIDDAFRRRFEKRVYIPLPS",
    "response#3": "PWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPSTIFIDEVDSLCSTRGSDSEHEASRRFKAELLIQMDGLNNASTGEDKSILVLAATNHPWDIDDAFRRRFEKRVYIPLPSVEERERLVKEK"
  },
  {
    "reference": "MSRIGNKVITLPAGVEIINKDNVVTVKGPKGELTREFNKNIGITVEGTEVTVTRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKALEMRGVGYRAQLQGSKLVLSVGKSHQDEVEAPEGVTFEVPTPTTINVIGINKESVGQTAAYVRSLRSPEPYKGKGIRYVGEFVRRKEGKTGK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.; This protein binds to the 23S rRNA, and is important in its secondary structure.2. The ideal protein would be one that plays a critical role in translation through rRNA binding, structural constituent of ribosome within ribosome, ribonucleoprotein complex. The designed protein sequence is ",
    "response#1": "PTVRGSVMNPNW",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#3": "PTVRGSVMNPNW"
  },
  {
    "reference": "MDDIRYADRIVSADEVKIAARKAQVFYGSNHALRDVDVDILGKTVTAFIGPSGCGKSTFLRCLNRMNDTIPGCRVEGKITLDGQDIYDTRVDPVQLRAKVGMVFQKPNPFPKSIYDNVAYGPRIHGLARSKAEIDEIVEGALRRAALWNEAKDRLSSPGTGLSGGQQQRLCIARAIATSPEVLLMDEPCSALDPIATAQIEELIDELRAQFSVVIVTHSMQQAARVSQRTAFFHLGRLVEYGETGQIFTNPRDPRTESYISGRIG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must be able to ATP binding, ATP hydrolysis activity, inorganic phosphate transmembrane transporter activity, ATPase-coupled phosphate ion transmembrane transporter activity.2. The ATP binding site should be stable and able to withstand changes in environmental conditions.3. The protein should be designed to catalyze the following reaction ATP + H2O + phosphate(out) = ADP + H(+) + 2 phosphate(in).4. The designed protein have ABC transporter domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MRAVDLTLGFGPKTVLEQVSLDFPARSVTTLLGPTGSGKTTFLRTLNRMNDKVSGFRHSGDVLLGGRTIFADRDLMEFRRSVGMLFQRPNPFPMSIMDNVVAGVRAHKMAPRKQFKTVAEARLTEVGLWDAVKDRLGDSPFRLSGGQQQLLCLARALAVNPDVLLLDEPTSSLDPTTTEKIEGLIRSLADRLTVIMVTHDLAQAARTGDRTAFFFEGRLVEEGPTKHLFLSPQHEETVRYR",
    "response#2": "MEARELSLRYGPKTAIHKVSLAILDRRVTALIGPSGCGKSTFLRSLNRMNDLISGTTHSGTILLDGTDIHDRGVDVVDLRRRVGMVFQKSNPFPKSIFENVAYGLRVGGMKDKAELATRVEKSLRGAALWDEVKDRLDESALGLSGGQQQRLCIARAMAVEPEVLLMDEPASALDPIATAKIEELIHELKSTYTIAIVTHNMQQAARVSDRTAFFYMGELVECGPTEQIFTNPREKRTEDYI",
    "response#3": "MRAVDLTLGFGPKTVLEQVSLDFPARSVTTLLGPTGSGKTTFLRTLNRMNDKVSGFRHSGDVLLGGRTIFADRDLMEFRRSVGMLFQRPNPFPMSIMDNVVAGVRAHKMAPRKQFKTVAEARLTEVGLWDAVKDRLGDSPFRLSGGQQQLLCLARALAVNPDVLLLDEPTSSLDPTTTEKIEGLIRSLADRLTVIMVTHDLAQAARTGDRTAFFFEGRLVEEGPTKHLFLSPQHEETVRYR"
  },
  {
    "reference": "MSSREIRIATRKSALALWQAEYVKARLEQAHPGLLVTLVPMVSRGDKLLDSPLSKIGGKGLFVKELETALLENNADIAVHSMKDVPMDFPQGLGLFCICEREDPRDAFVSNTFASLDQLPAGSIVGTSSLRRQAQLLARRPDLQIRFLRGNVNTRLAKLDAGEYDAIILAAAGLIRLGFEDRITSAISVDDSLPAGGQGAVGIECRSVDAEIHALLAPLHHEDTAVRVIAERSLNKHLNGGCQVPIACYAVLEGDDVWLRGLVGDPSGSVLLHADARAPQTSAQALGVQVAEALLEQGAADILKAVYGEANNE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should take into account the physiological conditions of the coproporphyrinogen-III from 5-aminolevulinate: step 2/4, protoporphyrin-IX biosynthesis pathway.2. The presence of dipyrromethane is necessary for the protein to function in the desired environment.3. The protein should have a fast turnover rate for H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+).4. The protein must exhibit the following characteristics: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps..5. The protein design must be able to perform hydroxymethylbilane synthase activity in order to contribute to protoporphyrinogen IX biosynthetic process. The designed protein sequence is ",
    "response#1": "VKIGTRGSKLAVVQALEAKQKLLDSFPNLSIEIVKIKTSGDKYANANLAEIGGKGLFIKEIEAELLENNIDMAVHSLKDVPAFFSWGLTIPCVLERLSPCDAFISHKHNSLESLPQQATIATSAIRRKVQLLNFRPDLNIVPLRGNVTTRLQNQSFDGIILAEAGLIRLEKHHLITEVLPPKVMLSAVGQGAICIQCRRNDVKLVIATRGSKLALWQAEHIKSLIETEHAGKVDVSLKIIKTKGDIILDVPLAKVGGKGLFVKEIEEALLDGSADLAVHSMKDVPMELPEGLFLGCIPEREEPSDTLLSVRYASLDALPHGARVGTSSLRRQSQLLALRPDLDIISLRGNVDTRLRKLMDGEFDAIVMATAGLKRLGLSAPHHEVLAPPRFLPAVGQGALGIEFREDRADLYSVDLAVRAERALLWRCEGGCQVPIAAHGQVSGDSLTLTGFIASVDGTRSVKESISGPVTDCEKLGIALAEKLL",
    "response#2": "LRVATRGSKLSIAQTMIALEAIKRVEPSLEYELVIVKTRGDIHQDKPFTAIGGKGLFEKEVNLAVLEGRADIAVHSLKDVPSAISPGLVLAMTPPRDPPYDVLVVRGGKEKTIWDLPSGAIVGTSSARRVAMLKHVRRDLVFKVLRGNVDTRLRKLEQGQYDAIVLAEAGLKRLGVDIEYWRIPPDILPPAPGQGIIGVYTLSSRSAVRAERALLWRCEGGCQVPIAAFGEVTGDELKLTGFIASVDGKVSVKGVVTGPADDCEKLGVKLAEQLASLVIASRESRLAMWQAEYVRAALQKYYPACDVSILGMTTRGDQILDRSLAKVGGKGLFVKELEVALAEGRADLAVHSLKDVPMELPPGFVLSAILEREDPRDAFVSNDYADLAALPAGAVVGTSSLRREASLRARFPHLVIQPLRGNLDTRLSKLDRGDYAAIILAAAGLKRLGLSERIRAVIAPETSLPAAGQGALGIESRVDRHDVERLNS",
    "response#3": "LRIGTRASQLALWQANWVKSELEKRYPDMTVTLTKIKTIGDKILDVPLAQVGGKGLFVKEIEEAMLRGEIDIAVHSMKDVPTEFPEGLGLYCITEREDPRDAVISNNVKFADLPQGARIGTSALRRQAQLLKVRPDLEMVIIRGNVQTRMDKLKTEGLDAVILAAAGLNRLGFADQITELLPTDLSLPAIGQGALGIECNLSNQDVEAVRAERALLWRCEGGCQVPIAAHGQVSGDSLTLTGFIASVDGTRSVKESISGPVTDCEKLGIALAEKLTAVKIGTRGSKLAVVQALEAKQKLLDSFPNLSIEIVKIKTSGDKYANANLAEIGGKGLFIKEIEAELLENNIDMAVHSLKDVPAFFSWGLTIPCVLERLSPCDAFISHKHNSLESLPQQATIATSAIRRKVQLLNFRPDLNIVPLRGNVTTRLQNQSFDGIILAEAGLIRLEKHHLITEVLPPKVMLSAVGQGAICIQCRRNDVKGE"
  },
  {
    "reference": "MTDSNGQKDNNQDQAQPADPVVSKPYIMPDDPEEGTNEALVREAAEARDKMLRTLAEMENLRRRTAKEVADARTYGVSAFARDVLEIADNLQRALDAVPAEARANADAGLKGLIEGVELTERSLINALEKNGVKKFDPQGEKFDPNFQQAMYEVPDPSVPAGTVVQVVQAGFMIGERVLRPALVGVSKGGAKPAPAAANGNEGGVA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex; Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.; GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE; It is the nucleotide exchange factor for DnaK and may function as a thermosensor2. A protein that can perform protein homodimerization activity, adenyl-nucleotide exchange factor activity in cytoplasm to support protein folding. The designed protein sequence is ",
    "response#1": "MQIQKVSIIVPTHNEEATIASCLESVRNQTFSDIEIIVVDNNSTDRTVEIARNFKDVRIIQERRRGVSAARNNGLKHAKGEIIAFTDSDCIVEKNWLKRLVEPFHDPRVIAVGGSIKISNESGSVLKRYLNFKPFAFDPKTGRFYFWGANMAVRKDVFEKVGLFDESFDYCEDVDLTWRIQKAGYKIVYTPEAIVYHKHRRTLKDYLHMQFMYGVGEVWLFKKYGQYFKQWKRSAWSLPVLVVLSLFRLWILIPIIPMLLWNAAYFFKFGDLKFLVLLPIGVMFLCQLLITGGVIARGRNKFLFALIVPIAYMLFYPLAIIYVVFKSKYKYTKFKRKTA",
    "response#2": "MVQDRSDLSQFQGPVRTVIVAAGSGTRLGFGRAKAFVPVAGKPMIQHTVEAFCRWPLIERVVVVAPEEEVSRARELVAGFAGKVTTVVGGASRQESVARGLEALAGCAEIMIHDAARPFAPPAVFERVLAAIEQAGAAVPGVAVTDTLKRVSESGAVVESVDRSQLRAVQTPQAFRRELLVAAHAAAVAAGIEATDDAAIAEWAGLPVRVVEGDYRNMKITTAEDLALAEALLQPVQGARLVPQVRPA",
    "response#3": "MTEQKIILVTGCSSGIGLECAKTLYAKGFRVFATARNLSKMEPLKQAGFETIALDVTSKESIDAAVQQVRNITGGKLDVLFNNAGFGYVAPALEVSDEEVRKQFETNFFGVIAMTQAVLKPMIAQKSGTIVQIGSVGGIVAVPFNAVYNATKFALEGASESLKYELKPLNIKVMLVEPGGVKTNWAANAAVLTKNVPAYSATADKALEAMAKGNRDGSPAEKVAQTIFKMATAPNPPLRILVGKGAFALGFVRKFLPDSLFDKIVLKQFGLNQATA"
  },
  {
    "reference": "MRSEPLAGCRILVAISGSIACVKLPALVSALVQRGAQVRCVLTPSAARLVSPVTLASLSRSPCHLEEDQWSHRAPRPLHVELAEWAELVLVAPLSATSLARWVQGSGDTLLSSTLLATEAPVLAAAAMNTAMWRSPGVRRNWQELQGFERVLTLGPAAGLLACDRQGDGRMAEPQQLLLGLETLRLHGWRRDWQGRRLLVSAGPTREWLDPARCLTNPSTGRMGVLLAQAARLRGAEVDLVHGPLNLDPLLLEGLRAYPAESGVAMGEALEQLQPAADAVAMAAAVADHGRAAPELQKLSKQDLLASLSGGWCEVPDLLAQLVARRQAGQRILGFAAHSGDVLPQARAKRLRKGCDLLFANPIDQPQAGFRVCSNEGWLLGPGERELRIEPAGKFAVAHQLISALGALLDPLTPPAEAPREGLAGRRAATDP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.2. The designed protein should have metal ion binding to facilitate coenzyme A biosynthetic process.3. The protein should have a low Km value for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A5. The designed protein have Flavoprotein domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MRIILGVGGGIAAYKSAFLLRLFKEAGHDVTVIPTEAALSFVGQATWEALSGHPVSTTVFDAVDTVNHVRLGQEADLVVVAPATADLLAQAAAGLAGDLLTNTLLATRGKVVFVPAMHTEMWQHPATQANVALLRSRGTAVMEPASGRLTGKDTGPGRLPDPEDIYAFALDAVLGAIGPTPLAGTRILVTAGGTIEPLDPVRYLGNRSTGRMGYAIAAEAVRRGAAVTLISGPSVLPEPAGVSRTMVETAREMHDAVLAAYNDVDVVIKAAAVADYRPKTAAKNKIKKQDGEITLVLTRNPDILLELGEKKQHQILVGFAAETQNIERYAREKLQKKNLDFLVANDVSEKGAGFGVETNHVKLVDAGGHIEQWPRLPKEELARQLVERIAAALA",
    "response#2": "MGKRVIITSGPTHEPIDPVRYIANRSSGKQGHALAAAAAAAGAEVILISGPVALPDPDGVETHRIETALEMRAAVEAALPADIFIGAAAVADWRVANTAEGKIKKGFRPPGFSLVENPDILASVARHGSMRPRLVVGFAAETADVLAHARAKLERKGCDIIVANDVSAGSDVMGSDENQITILFADGHVEELPRMSKDEVAAKIAEKIAAAL",
    "response#3": "LLGVTGGISAYKSVELARLLLKAGASVRVVMTEAAGRFVSPLTFQAVTGNPVRTALFDEKHEAAMGHIELARWADRMLIAPASADFMARMAQGVANDLLSTVYLACPSPVAIAPAMNTNMWQHPAVQHNLSVLEGRGVRVLGPASGEQACGDKGFGRMLEPMEILATTVLVGACGGIALYKVPLLISRLRELRAGVHVIMTEAATRFVSPLTFQTVSGNAVHTDLFARPQLWNVEHIALAERADLMVLVPATANILAKAATGIADDFLTTTLLAVTCPVLVVPAMNAAMYRHPATSRNIETLRGFGHEVLEPDTGRLASGAVGPGRYPSTERILAACLEALARPIDLAGVRAVVSAGGTREPLDPVRFLGNRSSGRQGVALAEALRDAGAEVELVAANIEVPAPDGVEVTAVTTASDLERVMRDAAAGADVVVMAAAVADFRPKEYVDSKIKKNPADDSAPVIELVRNPDILKGLVEDRGARAQRNNEGSAANEPGLLPRIIVGFAAETGDENGSVLEYGRAKLERKGADLLVVNEVSETRGFGTPDNTVAILDAEGNEIARAEGSKLVVANALLDHVGALLG"
  },
  {
    "reference": "MDEATGETETQDFMNVESFSQLPFIRRPKDKNPKPIRVFGKDFTGRDFSITTGQEDYTDPYQTKNKEEEEEEDQTGDNSTDNNSISHNRRFECHYCFRNFPTSQALGGHQNAHKRERQLAKRGVSSYFYHPDNNPYSYRHYPSWTNGPLTAARSYGGFSSGPKPSGYYTRPSYGSQLGLWRLPPRVQGVYNSNAAFTSNGSSSSSNSTLPLLTRSQTQLSSQVGGSAAQNRMSSYGYGLSPNVQDHVSLDLHL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.2. A protein with transcription cis-regulatory region binding is needed to enhance response to gibberellin, gibberellic acid mediated signaling pathway in a specific context.3. The protein must exhibit the following characteristics: Controls trichome cell division indirectly by acting downstream of a key endoreduplication regulator SIAMESE (SIM).; Acts downstream of the key regulator STICHEL (STI) in an endoreduplication- independent pathway; Does not play a significant role in the cytokinin response; Controls trichome branching through GA signaling. The designed protein sequence is ",
    "response#1": "YMAERVVGTGSFGIVFQAKCLETGETVAIKKVLQDRRYKNRELQLMRVMDHPNVVCLKHCFFSTTSKDELFLNLVMEYVPESLYRVLKHYSSANQRMPLVYVKLYMYQIFRGLAYIHNVAGVCHRDLKPQNLLVDPLTHQVKICDFGSAKQLVKGEANISYICSRFYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGENAVDQLVEIIKVLGTPTREEIRCMNPHYTDFRFPQIKAHPWHKIFHKRMPPEAIDFASRLLQYSPSLRCTALEACAHPFL",
    "response#2": "TTALVNELVGVVSDEKLLELLELALSSDTAETVKRARELLDLGADPIVLMSQLASLIMDIIAGTYKVVDEKYSNAFFDGRNLTEADMEGLKHALKLLSEAEKQLRVSNDRSTWFTATLLQS",
    "response#3": "KQTISYMAERVVGTGSFGIVFQAKCLETGETVAIKKVLQDRRYKNRELQLMRVMDHPNVVCLKHCFFSTTSKDELFLNLVMEYVPESLYRVLKHYSSANQRMPLVYVKLYMYQIFRGLAYIHNVAGVCHRDLKPQNLLVDPLTHQVKICDFGSAKQLVKGEANISYICSRFYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGENAVDQLVEIIKVLGTPTREEIRCMNPHYTDFRFPQIKAHPWHKIFHKRMPPEAIDFASRLLQYSPSLRCTALEACAHPFFDEL"
  },
  {
    "reference": "MDKNVLLTPAQMAAADRAAISLGISGDELMEAAGLAVANAVRDRWQPGPVVVLCGPGSNGGDGFVAARHLAQWGWPVRLGLLGEPAALAGSAAHHAALWEGAVEPVSVSLLDGASIVVDALFGAGLSRPLQDEAYAVVQEIAARKLPVCAVDVPSGVDGGTGQVLGAAAPAEITVTFFRRKPGHVLLPGRVLCGRLVLADIGIPGSVFEQIPSTVFRNSPRLWLSAYPWPRMDDYKHQRGHVLVVGGEIMTGAARLSAMAAARVGAGLVTLVVPEPAWAVYAAALTSIMVQPLDDVGSLPQMLEDGGRNVIVVGPGAGVSEATRQHVLAALNTGRGVVLDADGITSFRTVPQTLFSAIRGPCVMTPHEGEFGRVFTCQGSKLERARAAAAQCGAVIVLKGPDTVIASPDGRVIINDNAPPDLATGGTGDVLAGLVAGLMAQGLEPFFAAAAAVWLHGRAAQRFGPGLIAEDLPAMLPQVLRQLKQASMSHVAHLVPVPDRA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein contains novel YjeF N-terminal domains that confer a unique function or activity.2. The designed protein should have NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding to facilitate nicotinamide nucleotide metabolic process.3. The protein should be able to bind (6S)-NADPHX ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "LLIIGGSETFYGAPTLAAMSAMRASVDLVYVAAPEETAKTIASYSPDLITIKLKGRNLNPDNVKELMPWIERANAVVLGPGMGMSDETVQFSQEIVNLLLGLNKPVVLDADALKANKGRRLSKSFVITPHAGEFAIYFGMQLSMDVKERIGQVIEGSKKCDCVVLAKGYVDVIADGSRFKLNKTGNPGMTVGGTGDTLAGLVGAFLAMGLEPYKAAYLGAFVNGLAGSLAYAELGPHITASDLVYRII",
    "response#2": "VGIVAGSAQYPGAAVLAVAGALRGGAGAVRYVGPAADAVLARYPETLIGPGRVQAWVIGPGLGEGRAGEVADVLAGDAAVLVDADGLRGLDPQVLRARTAPTLLTPHAGEAAALLGVAREAVEAGRLAAVRRLAERYGATVLLKGSTTLVASAGVRPVRVNPTGTPWLATAGSGDVLSGLAGSLLAAGLSAADAGSVAAYLHGLAARL",
    "response#3": "LLIIGGSETFYGAPTLAAMSAMRASVDLVYVAAPEETAKTIASYSPDLITIKLKGRNLNPDNVKELMPWIERANAVVLGPGMGMSDETVQFSQEIVNLLLGLNKPVVLDADALKANKGRRLSKSFVITPHAGEFAIYFGMQLSMDVKERIGQVIEGSKKCDCVVLAKGYVDVIADGSRFKLNKTGNPGMTVGGTGDTLAGLVGAFLAMGLEPYKAAYLGAFVNGLAGSLAYAELGPHITASDLVYRIG"
  },
  {
    "reference": "MEKKDIKSMDLEELTQELLAMGEKKFRAGQIYGWMHVHLVRSIDEMTNISQKLREQLKENYAFTAVKLLERQISRIDGTQKFLFRLADGNVVESVWMQYKHGNSVCISSQVGCRMGCRFCASTLDGLTRNLLPSEMLDQIYAITLLTGERVSNVVVMGTGEPFDNFDNLKKFIRLLTDENGLHISQRNVTVSTCGLVPRIYELAEENLQITLALSLHATTDEKRRKLMPIANKYSIDELMEACAYYHQKTGRRVTFEYSMVKGVNDSAEDAQQLTALAKRVGSHINLIPVNPIKERDFVAPDTHYVQVFKNKLEKNRINVTIRREMGRDIDGACGQLRKRHLDEMGGEDSSC",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A key design requirement for the protein is the optimization of the S-methylcysteine intermediate to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.2. The protein contains novel Radical SAM core domains that confer a unique function or activity.3. The designed protein must possess tRNA binding to support rRNA base methylation under certain conditions.4. The protein should be able to bind [4Fe-4S] cluster ligand in a variety of conditions5. The protein must bind to [4Fe-4S] cluster in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "SKTRMTACISSQVGCSLACEFCATGKLKLLKNLSAGEIVDQVVHLKNLAENKYQQNLSNIVYMGMGEPLLNYKNMMDSIDKITSEDGLGMSPKRITVSTAGIAKMIKKLGDDNVKFNLAVSLHAANDKKRDKIMAINETNNLEALAEAIAYFYGKTGTRVTYEYIIFKDFNDELEDAQELAEFCKNVPCKVNIIEYNTIDDGIFQQADARKVDRFAAYLEEKNIIVNVRRSRGKDIDGACGQLAA",
    "response#2": "QEYGLSVCVTTQVGCNIGCTFCASGLLKKQRDLTAGEIVAQIMLVQHYFDERGEEERVSHVVVMGIGEPFDNYDNVMNFLHVINDDKGMAIGARHITVSTSGLVPKIKKFAENGLQVNLAISLHAPNNEVRSSIMRINRSFPIEKLMAAVDYYIEQTNRRVTFEYIMLSGVNDRPEHAQELADLMKDKKKLVYVNLIPYNPVSEHDQYSRSSKADVLRFYDVLKKNGIAIAEEIE",
    "response#3": "TTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVKVQQALDEFEERVSHVVIMGIGEPFDNYDEMMSFLKIINHDKGLNIGARHITVSTSGIIPKIYKFADEKLQINFAISLHAPNTELRTKLMPINKAYKLPDLMEAVRYYVDKTGRRVSFEYGLFGGENDQVEHAGWVKKNVTSKPSLIGLDRVALADRLTDLGVPERQIRMRVAQLWNRLYVGGAQTFDDMTTLSKDLRHALDSQVSLDRPEIVSEQISTDGTRKWLLRLAPGRPGEAAPEIETVYIPEEDRGTLCVSSQVGCTLTCTFCHTGTQRLVRNLTPGEIVGQILIARDRIGEWPGATRPTDGPLPNAERLITNIVLMGMGEPLFNFDNVRQAMEIAADGDGISVSKRRITLSTSGIAPMIPRCGVELGVMLAISLHAVRDDLRDVLVPINRKYPIAELLDACRAYPSLSNAKRITFEYVMLRGVNDSLADAKALVRLLAGIPAKINLIPFNPWPGAPYESSEWEQIEKFAEIINKAGYASPVRTPRGRDIMAACGQLKSASEKMHARKQHAA"
  },
  {
    "reference": "MFALFIWSFMAIILGIDPGSRVTGYGIIRQTGRTLEYLGSGAIRTQVEDLPTRLKRIYAGVTEIITQFRPDMFAIEEVFLAKNPNSALKLGQARGTAIVAAVNQNLPVFEYAARLVKQTVTGSGSADKVQVQDMVTRILRLSDKPQADAADALAIAITHAHTIQHSLQVATSAKSTENHEKTTALLRTRYSRGRFRLKI",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. The protein should be designed to have high stability and activity towards Endonucleolytic cleavage at a junction such as a reciprocal   single-stranded crossover between two homologous DNA duplexes   (Holliday junction)..3. The designed protein should have crossover junction endodeoxyribonuclease activity, magnesium ion binding to facilitate DNA recombination.4. The protein must exhibit the following characteristics: The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair; Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange; HJ branch migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.; The consensus cleavage sequence is 5'-(A/T)TT(C/G)-3'. The designed protein sequence is ",
    "response#1": "MRIILLGAPGAGKGTQAKFIMEKYGIPQISTGDMLRAAIKSGSELGKEAKTIMESGGLVSDELIIGLVKERIAQDDCKKGNG",
    "response#2": "MRGNGAADKKQVQHMVRYLLKLPSAPNTDAADALAIARAAAVR",
    "response#3": "MRGKGNASKKQVQLMVSKLLRVPDLLAEDNEDIADAFALAKELGADVVYGPDFMKLVQAQKPDIIVKGQDYRDLDILGAEIVKKNGGRVELVHSYAEASTSTITSRIKRG"
  },
  {
    "reference": "MDRYILSLDEGTTSARAIIFDKNSNVLGIGQYEFPQYYPKPGWVEHNPEEIWEAQVKAIKTAIEKAKIEANQIAAIGVTNQRETTVIWKKDGKPVYNAIVWQCRRTAEMVEEIKREYSDVIKEKTGLVPDAYFSASKIAWLLDNVPGLREKAEKGEVMFGTIDTFLIYRLTGEHVTDYSNASRTMLFNINKLEWDEELLEIFKIPDGILPDVKESSEIYGYTKILGPEIPVSGDAGDQQAALFGQAAFEEGMVKATYGTGNFILANTGRMLLYSDNLLTTVAWGINGKVNYALEGSIFITGAAVQWLRDGIKIISNAAETEELATKLESNEGVYFVPAFVGLGAPYWDQFARGLIIGITRGTGREHLARATLEAIAYLTRDVIEEMEKLIQIKELRVDGGATVNNFLMQFQADILNKKVIRPVVKETTALGAAYLAGLAVDYWESMEEISQLWRAERVFEPQMDEKTREKLYRGWKEAVKRALGWAKVVS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a high specificity for ATP, substrate binding, with minimal off-target effects.2. The protein design should consider the endogenous proteins and enzymes present in the sn-glycerol 3-phosphate from glycerol: step 1/1, Polyol metabolism, glycerol degradation via glycerol kinase pathway pathway to avoid interference with native metabolic processes.3. A protein with ATP binding, glycerol kinase activity is needed to enhance phosphorylation, glycerol-3-phosphate metabolic process, glycerol catabolic process in a specific context.4. The protein must exhibit the following characteristics: Key enzyme in the regulation of glycerol uptake and metabolism; Catalyzes the phosphorylation of glycerol to yield sn- glycerol 3-phosphate..5. The protein should have a fast turnover rate for ATP + glycerol = ADP + H(+) + sn-glycerol 3-phosphate. The designed protein sequence is ",
    "response#1": "STYGTGCFMLLNTGDQIIHSRNRLLATIAYRLNDTVTYGLEGSIFIAGAAVKWLRDPLHLIEKANDSESMASSVEDTGGVYLVPAFTGLGAPYWDPNARGALFGLTRNTQREHIVRAALEAVCYQSKDLVRAILNDGANLTTLRVDGGMAANNWLLQFLSDILGVNVDRSRCIESSALGTAFLAGLELGYYNEEDIKNTYGTGLFMLLNTGAKPKWSDNGLITTVCYQIENQKPVYALEGSVAMGGSLVQWLRDNLQLIPNAPSVENMAKSVEDNGGVYFVPAFSGLFAPRWRPDARGAIVGLTRFANRNHLARAVLEATAYQTREVLDAMVTDSGVDINALKVDGGMVMNEFLMQFQSDIINTEVVRPKNIETTALGAAYAAGLIAAHDVIGKNTYGTGLFMLLNTGAKPKWSDNGLITTVCYQIENQKPVYALEGSVAMGGSLVQWLRDNLQLIPNAPSVENMAKSVEDNGGVYFVPAFSGLFAPRWRPDARGAIVGLTRFANRNHLARAVLEATAYQTREVLDAMVTDSGVDINALKVDGGMVMNEFLMQFQSDIINTEVVRPKNIETTALGAAYAAGLGL",
    "response#2": "STYGTGCFMLLNTGDQIIHSRNRLLATIAYRLNDTVTYGLEGSIFIAGAAVKWLRDPLHLIEKANDSESMASSVEDTGGVYLVPAFTGLGAPYWDPNARGALFGLTRNTQREHIVRAALEAVCYQSKDLVRAILNDGANLTTLRVDGGMAANNWLLQFLSDILGVNVDRSRCIESSALGTAFLAGLSAGLFNAPDEYFSAGKLVWLLEKNTYGTGCFMLMHTGHSFQSSANGLITTSAAQTTAQPEFAMEGSVFVGGAVVQWLRDGLRAIPSSSEVQALAESVPDAGGVMVVPAFTGLGAPYWKPDARGTITGLSRGSTLAHIARAALESIAFQSAALLQAMSRDAVQAGGSAVAELRVDGGACVNDLLMQFQADLLGIAVVRPAVIETTALGAAYLAGLHEYWFRHPAEWQPESRFAQSFSPETLPRALTGYEQRFAAYSRWKKSL",
    "response#3": "STYGTGCFMLLNTGDQIIHSRNRLLATIAYRLNDTVTYGLEGSIFIAGAAVKWLRDPLHLIEKANDSESMASSVEDTGGVYLVPAFTGLGAPYWDPNARGALFGLTRNTQREHIVRAALEAVCYQSKDLVRAILNDGANLTTLRVDGGMAANNWLLQFLSDILGVNVDRSRCIESSALGTAFLAGLKAGVFENRESSTYGTGCFMLLNTGDQIIHSRNRLLATIAYRLNDTVTYGLEGSIFIAGAAVKWLRDPLHLIEKANDSESMASSVEDTGGVYLVPAFTGLGAPYWDPNARGALFGLTRNTQREHIVRAALEAVCYQSKDLVRAILNDGANLTTLRVDGGMAANNWLLQFLSDILGVNVDRSRCIESSALGTAFLAGLELGIYPEPEILAKRLSKDISLTTPSLSLEEANGIYNKWLKVVELAK"
  },
  {
    "reference": "MFTMKKSLLLIFFLGTINLSLCEEERNADEDEKRDGDDESDVEVQKRFMGTALKIAANVLPAAFCKIFKKC",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in hemolysis in another organism, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "NINISCETDGYLTKMTCRWSANPMQSLVGSSWKLRYYRRSLYCSERPSIHPVSEPKDCHLQDDGFYECIFQPIFLFSGYTMWIRINHTLGSLDSPPTCVTPS",
    "response#2": "NWNVQCWVTGDLKLLICAVEPSRQNPCKNYDLHLFYALLEVAGGSRLAPQKGSFEIVQCNCSSEDRWECRVPVPAAKLHDSFLMYLKITAAWATFWSPLMSVQPV",
    "response#3": "NWNVQCWVTGDLKLLICAVEPSRQNPCKNYDLHLFYALLEVAGGSRLAPQKGSFEIVQCNCSSEDRWECRVPVPAAKLHDSFLMYLKITAAWATFWSPLMSVQPV"
  },
  {
    "reference": "MLHRSFFRFAALADKKAFMELVKALRYRTEAPISDCSAALTEAAGDMDAAMQLLRKRGVARAMKKGDCVTEHGFVVSCVGSTPASGAAIITMCSETDFAARNEHFQRTCVQARDQLRKLMDATNGAVLANPEEAAKQLSDIMGEELRAAIAVLGENMRIRSIAPLVPAPHMSERLLVGSYTHGVLNVDGVGRIVGLVAVSQVRENEVISKDVLTSIGRHFVATSGAEGNYAHQNFFGSETETVGKWLKHHGLTFSSSLVQEFGKEPVVHTAAEPHQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP; It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome..2. The protein should be optimized for mitochondrion-specific folding and stability, while maintaining translation elongation factor activity. The designed protein sequence is ",
    "response#1": "ACMLELNCESDFVAQNPKIQRLALSSASSILHSLNRVNADSTEQRNVSTDLDSLKKIILHSDKDSSSNLGNTLFDAFSNATSTTGERIELSRAIVFQRTKPQHQLGRYTHGTDVRVHSSLGRVGCLVSLSNAQKAGLADDIAREFVAQDPESIDII",
    "response#2": "MPACMLELNCESDFVAQNPKIQRLALSSASSILHSLNRVNADSTEQRNVSTDLDSLKKIILHSDKDSSSNLGNTLFDAFSNATSTTGERIELSRAIVFQRTKPQHQLGRYTHGTDVRVHSSLGRVGCLVSLSNAQKAGLADDIAREFVAQDPESIDE",
    "response#3": "MSAFLYEINSETDFVSKNEHFQQLVKVLGEIILKNQLSNAKDLLAFNYQNKTVQELLLEKTSVLGENITLKRVLKVTKKPQESFGTYKHQGGRISVLVVLKNDCPSVSEDIAMHIAASKPQFLTPDKVDPTFLAEEKKILHKQTAKELSDKPAQMIEKIVENRLGKMLKDMCLSEQPFVKNADQKVKDYLKANNTDVVSYVRWEMK"
  },
  {
    "reference": "MITIDFSKGLVPTIILDDHNGEVLMLAYMSEESYQKTVETGYTWFFSRSRNELWNKGATSGHTQKVKQIWTDCDNDTLLIRVNQTGPACHTGKKSCFFHLIKEGD",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The presence of Zn(2+) is crucial for the protein to function optimally under specific conditions.2. The ideal protein would be one that plays a critical role in histidine biosynthetic process through phosphoribosyl-AMP cyclohydrolase activity, magnesium ion binding, zinc ion binding within cytoplasm.3. For general function, the protein need meet that Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.4. The designed protein should interact with enzymes and substrates within the Amino-acid biosynthesis, L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9, L-histidine biosynthesis pathway to enhance or regulate its activity.5. The Mg(2+), Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.6. We need a protein that can perform 1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-   D-ribosyl)-5-[(5-phospho-beta-D-   ribosylamino)methylideneamino]imidazole-4-carboxamide with high efficiency. The designed protein sequence is ",
    "response#1": "MMAWMNRQSLEMTLEKKKACYWSRSRQKLWLKGESSGNMQNVHDILIDCDGDTLLLKVSQTGGACHMGYQSCFHRRLTEEDGVELHPCEVREDTPVFSLGSLVSEIGMKRLGYAGEMSAPERNQKLSSVWDFFLDKAVFSSEGQARPQSP",
    "response#2": "MMAWMNRESLRLTAEKQQAIYWSRSRNQLWHKGETSGHVQHVREIRLDCDEDVIVLQVVQEGGIACHTGRESCFLSSNNLQSPKILGADVXFXEXPXKXEXAALXKXGXFPIIPCNLCGSQENLQRXQIXKDLQHWEKSXPGRFDTMIRALTNIQPSHLLDXDLFXFSXLSSXNNXHXSXDXNXXTXQX",
    "response#3": "MLAWMNREALQKTAELGRAVYFSRSRNKLWFKGEESGHVQIVHEIRIDCDQDVILLKVTQTGHTPGIACHTGRHSCFLDPDAPMLRAALERVLARPRRLPAPPDSAGGA"
  },
  {
    "reference": "MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLDHNETSAPDLTRHALSTSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYCLDRWIVDYNKMKCPVCRHRFLPKEKYTQSDWGSGSDWFSDEVESTN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that can modulate the overall flux of metabolites through the protein ubiquitination pathway, enhancing or suppressing its activity as needed.2. The protein must exhibit the following characteristics: Probable E3 ubiquitin-protein ligase that may possess E3 ubiquitin ligase activity in vitro..3. The designed protein must be able to perform metal ion binding, ubiquitin protein ligase activity under specific conditions. The designed protein sequence is ",
    "response#1": "KLQRILEDLSEPPFDPGQYINLYTIIYDMCLQQPPNDYSQELYNKYRGVVDHYNKETVLPSMRERHGEYMLRELVKRWANHKILVRWLSRFCFYLDRFYVARRGLPTLNDVGFTSFHDLVYQEIQSEAKDVLLALIHKEREGEQIDRTLVKNVIDVYCGNGVGQMVIYEEDFESFLLQDTASYYSRKASRWSQEDSCPDYMLKAEECLKLEKERVTNYLHSTTEPKLVEKVQNELLVVVAKQLIENEHSGCLALLRDDKMGDLSRMYRLYRLIPQGLEPIADLFKQHVTAEGNALIKQAADAATNQDASASQVLVRKEIELHDKYMVYVDECFQKHSLFHKLLKEAFEVFCNKTVAGASSAEILATYCDNILKTRGGSEKLSDEATEITLEKVVNLLVYISDKDLFAEFYRKKQARRLLFDRSL",
    "response#2": "KLQRILEDLSEPPFDPGQYINLYTIIYDMCLQQPPNDYSQELYNKYRGVVDHYNKETVLPSMRERHGEYMLRELVKRWANHKILVRWLSRFCFYLDRFYVARRGLPTLNDVGFTSFHDLVYQEIQSEAKDVLLALIHKEREGEQIDRTLVKNVIDVYCGNGVGQMVIYEEDFESFLLQDTASYYSRKASRWSQEDSCPDYMLKAEECLKLEKERVTNYLHSTTEPKLVEKVQNELLVVVAKQLIENEHSGCLALLRDDKMGDLSRMYRLYRLIPQGLEPIADLFKQHVTAEGNALIKQAADAATNQDASASQVLVRKEIELHDKYMVYVDECFQKHSLFHKLLKEAFEVFCNKTVAGASSAEILATYCDNILKTRGGSEKLSDEATEITLEKVVNLLVYISDKDLFAEFYRKKQARRLLFDRSS",
    "response#3": "KLQRILEDLSEPPFDPGQYINLYTIIYDMCLQQPPNDYSQELYNKYRGVVDHYNKETVLPSMRERHGEYMLRELVKRWANHKILVRWLSRFCFYLDRFYVARRGLPTLNDVGFTSFHDLVYQEIQSEAKDVLLALIHKEREGEQIDRTLVKNVIDVYCGNGVGQMVIYEEDFESFLLQDTASYYSRKASRWSQEDSCPDYMLKAEECLKLEKERVTNYLHSTTEPKLVEKVQNELLVVVAKQLIENEHSGCLALLRDDKMGDLSRMYRLYRLIPQGLEPIADLFKQHVTAEGNALIKQAADAATNQDASASQVLVRKEIELHDKYMVYVDECFQKHSLFHKLLKEAFEVFCNKTVAGASSAEILATYCDNILKTRGGSEKLSDEATEITLEKVVNLLVYISDKDLFAEFYRKKQARRLLFDRSI"
  },
  {
    "reference": "MAFQEFTSTAVQLFDKWMESSDPRTKGWLLMSNPIPQMLIIVFYIYFVISLGPKIMENRKPFDLKRVLIVYNIFVVSLSVYMCYEFLMAGWGTGYTFGCDLVDYSQSPKAMRMASVCWLYYFSKFIVMLDTVFFVLRKKPKQITFLHVFHHSIMPFTWWFGVRFSPGGLGTFHALLNCIVHVIMYTYYLLSALGPSFQRFLWWKKHLTSLQLIQFVLVTVHISQYFFMKDCPYPYPLFMYIIALYGIIFLLLFLNFWHHAYTKGKRLPKILQKTISPKNNNDIHHKKE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that May participate to the production of saturated and polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.; Little or no activity toward C22:0-, C24:0-, or C26:0-CoAs2. The protein should have a low Km value for a very-long-chain acyl-CoA + H(+) + malonyl-CoA = a very-long-   chain 3-oxoacyl-CoA + CO2 + CoA.3. The protein design must be focused on enhancing the efficiency of fatty acid elongation, monounsaturated fatty acid, long-chain fatty-acyl-CoA biosynthetic process, unsaturated fatty acid biosynthetic process.4. The protein should be optimized for activity in fatty acid biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MSCCGGNCGCGSGCNGCNCKSCTNCYRDVPSTVVTEHPQEDTCLVGGAPGCKCGDNCKCKPNCTCKGNSP",
    "response#2": "MLSNVVTIILAATSLVTAIPTTAVEKRAEDLAPVCRGYHVNNTRGRIIIWNSEYTWHEICSYPRCNDACGKYQSLVNVCPDGWNLHGLECDWPEAYHLSYRIDNTKQ",
    "response#3": "MSIVCRSAGCRQRHRPGHRHCRALLKLVSGDSSSAAGYGQGGDLFVSDSRIDRSAGSGAGGGVVARGGGAVTVTGGEITGNRADGAGGGIYAGGAPTVTISGGTISGNTADKAGGGVYATADTVTISGGTVSGNTALDGGGVYATASSLTISGSTVTGNTADRAGGGVYAGGA"
  },
  {
    "reference": "AQSVPWGISRVQAPAAHNRGLTGSGVKVAVLDTGISTHPDLNIRGGASFVPGEPSTQDGNGHGTHVAGTIAALNNSIGVLGVAPSAELYAVKVLGASGSGSVSSIAQGLEWAGNNGMHVANLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGAGSISYPARYANAMAVGATDQNNNRASFSQYGAGLDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGAAALVKQKNPSWSNVQIRNHLKNTATSLGSTNLYGSGLVNAEAATR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The stability of the protein-Ca(2+) complex should be optimized to maximize enzymatic activity.2. The design of the protein must focus on the precise arrangement of amino acids in the Charge relay system to enable effective catalysis of Hydrolysis of proteins with broad specificity for peptide   bonds, and a preference for a large uncharged residue in P1.   Hydrolyzes peptide amides..3. The protein contains novel Peptidase S8 domains that confer a unique function or activity.4. The target protein must exhibit helix-helix-strand-strand-strand-strand-helix-strand-strand-strand-helix-strand-strand-helix-strand as its primary conformation.5. The protein must exhibit the following characteristics: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides..6. The designed protein should have a binding site that is specific to the target Ca(2+) ligand.7. A protein that contributes to proteolysis by functioning in extracellular region with serine-type endopeptidase activity, metal ion binding. The designed protein sequence is ",
    "response#1": "IVGGYTCEENSVPYQVSLNAGYHFCGGSLISDQWVVSAAHCYKSRIQVRLGEYNIDVLEGNEQFINSAKVIRHPNYNSWILDNDIMLIKLSSPAVLNARVATISLPRACAAPGTQCLISGWGNTLSSGTNYPELLQCLDAPILTQAQCEASYPGQITENMICAGFLEGGKDSCQGDSGGPVVCNGELQGIVSWGYGCAQKNKPGVYTKVCNFVDWIQSTIIA",
    "response#2": "VVGGEDAVPNSWPWQVSLQYLKDDTWRHTCGGSLITTSHVLTAAHCINKDFTYRVGLGKYNLTVEDEEGSVYAEVDTIYVHEKWNRLFLWNDIAIIKLAEPVELSNTIQVACIPEEGSLLPQDYPCYVTGWGRLWTNGPIAEVLQQGLQPIVSHATCSRLDWWFIKVRKTMVCAGGDGVISACNGDSGGPLNCQAEDGSWQVHGIVSFGSSSGCNVHKKPVVFTRVSAYNDWINEKIIN",
    "response#3": "VVGGTEAGRNSWPSQISLQYRSGGSRYHTCGGTLIRQNWVMTAAHCVDYQKTFRVVAGDHNLSQNDGTEQYVSVQKIVVHPYWNSDNVAAGYDIALLRLAQSVTLNSYVQLGVLPQEGAILANNSPCYITGWGKTKTNGQLAQTLQQAYLPSVDYAICSSSSYWGSTVKNTMVCAGGDGVRSGCQGDSGGPLHCLVNGKYSVHGVTSFVSSRGCNVSRKPTVFTQVSAYISWINNVIIN"
  },
  {
    "reference": "FQPSLVLDMAQVLLDNYTFPENLM",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina.2. The protein should be optimized for extracellular region, interphotoreceptor matrix-specific folding and stability, while maintaining retinal binding. The designed protein sequence is ",
    "response#1": "VRCTIEAGYPGVLSSRDKYGRVVMLFNIENWQSQEITFDEILQAYCFILEKLLENEETQINGFCIIENFKGFTMQQAASLRTSDLRKMVDMLQDSFPARFKAIHFIHQPWYFTTTYNVVKPFLKSKLLERVFVHGDDLSGFYQEIDENILPSDFGGTLPKYC",
    "response#2": "VRCTVEAGYPGVLSTRDKYGRVVMLFNIENWDSEEITFDEILQAYCVILEKLLENEETQINGFCIIENFKGFTMQQAAGLRPSDLRKMVDMLQDSFPARFKAIHFIYQPWYFTTTYNVVKPFLKSKLLQRVFVHGEDLSSFYQEFDEDILPSDFGGTLPKYR",
    "response#3": "LRCTIEAGYPGVLSSRDKYGRVVMLFNIENWHCEEVTFDEILQAYCFILEKLLENEETQINGFCIVENFKGFTMQQAAGLRPSDLKKMVDMLQDSFPARFKAIHFIHQPWYFTTTYNVVKPFLKNKLLQRVFVHGDDLDGFFQEIDENILPADFGGTLPKYR"
  },
  {
    "reference": "MSPSLSGRTPRPKKRLGQHFLKDANTARIVAAGLTERDVVLEIGPGRGFLTAFLAERAGLVHAVEIDPDVLPELRRAVGARGNVRIHEADALRFDYGALSPPPNRLAANLPYNIASPLVLRLLEEVPSLERMRFMVQLEVALRMTARPGSKDYGAYAVLIQLLSRPEVAHRVSPRVFDPPPRVRSAVVELERRRDAPEDYRGVKELVAAAFRSRRKRLPNNLPGPLRERAEEALAGLGHGPDARAEELSPEDFVALYRRISP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that localizes to cytoplasm and can perform RNA binding, 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity with high specificity and efficiency.2. The protein must exhibit the following characteristics: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle.3. The designed protein should have a high specificity for S-adenosyl-L-methionine binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MAEKIVGTLTAPPEAHVVEVGAGTGVLTERLAERHDRLTALEIDERAVEVLRERVPEADVRETDVRETDWAALADEKGGPLRVISNTPYYLTSPILFALLGQRDCLAEAVLTMQKEVAERIVAEPSTKAYGILSVLLQLFAEPTLCFTVPPQVFSPQPDVTSAVVRIRFGS",
    "response#2": "VIDSIVSAINPQKGQAMVEIGPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAEKMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRLSVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHA",
    "response#3": "VIDSIVSAINPQKGQAMVEIGPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAEKMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRLSVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHR"
  },
  {
    "reference": "MGGKWSKSSLGGWPTVRERMRRAKTTTEAEPAAQGVGAVSRDLEKHGAVTSSNTAANNADCAWVEAQEEGDEVGFPVRPQVPLRPMTYKAALDLSHFLKEKGGLEGLVWSQKRQDILDLWVYNTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPDKVEEANKGENNCLLHPLSQHGMDDPEKEVLVWKFDSHLAFQHAARELHPEFYKNC",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should contain at least one PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif to achieve its intended function.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in host cell Golgi membrane that is critical for suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I. The designed protein sequence is ",
    "response#1": "MGLGVPFNIASYALLTYMIEQVSGLKLDEFVHTLGDMYQKYIDDLEAMGLKPEIKLRL",
    "response#2": "GNRKXTXG",
    "response#3": "MF"
  },
  {
    "reference": "MVSAEAEAARRPARFDVRGGPLKLAGRLFWGALGAGAEGAADPSAQPSRGLDRAAPRALRRSPLPSAGTGLAGGRERRLLRVAAVAGAESGQIPPGGRVAVAVMDVSRGVSARGSAFERWDERVAAPPACPPPAPRLFSAPSSPPTSLPLPFSPPCCGSALRGPQSWRRLSLRAGPAQVRGARVPRVPSPRPGPAPVAAPAPLRRGRDSARRHFCTRLLLSVLFLRAPLRAAALGTDPRPGTGRCWRVPGLRPRGVPAGALEAFGCIWTGGPGRRGSEDMRARPRRPSSSRSFKRSDPVNPYHVGSGYVFAPATSANDSEISSDALTDDSMSMTDSSVDGIPPYRVGSKKQLQREMHRSVKANGQVSLPHFPRTHRLPKEMTPVEPATFAAELISRLEKLKLELESRHSLEERLQQIREDEEKEGSELTLNSREGAPTQHPLSLLPSGSYEEDPQTILDDHLSRVLKTPGCQSPGVGRYSPRSRSPDHHHHHHHSQYHSLLPPGGKLPPAAASPGACPLLGGKGFVTKQTTKHVHHHYIHHHAVPKTKEEIEAEATQRVHCFCPGGPEYYCYSKCKSHPKALETMPGEQFGGSRGSTLPKRNGKGTEPGLALPAREGGAPGGAGAMQLPREEGDRSQDVWQWMLESERQSKPKPHSAQSTKKAYPLESARSSPGERASRHHLWGGNSAHPRTTPRAHLFTQDPAMPPLTPPNTLAQLEEACRRLAEVSKPPKQRCCVASQQRDRNHSATVQAGATPFSNPSLAPEDHK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design must be able to perform I-SMAD binding in order to contribute to intramembranous ossification, positive regulation of protein phosphorylation. The designed protein sequence is ",
    "response#1": "LKRRQPKKTNELPHPNKLPGIFDDGHGSRGREVCRRHELYVSFRDLGWLDWVIAPQGYSAYYCEGECAFPLDSCMNATNHAILQSLVHLMKPDVVPKACCAPTKLSATSVLYYDSSNNVILRKHRNMVVKACGCS",
    "response#2": "LKRRQPKKTNELPHPNKLPGIFDDGHGSRGREVCRRHELYVSFRDLGWLDWVIAPQGYSAYYCEGECAFPLDSCMNATNHAILQSLVHLMKPDVVPKACCAPTKLSATSVLYYDSSNNVILRKHRNMVVKACGCS",
    "response#3": "LKRRQPKKTNELPHPNKLPGIFDDGHGSRGREVCRRHELYVSFRDLGWLDWVIAPQGYSAYYCEGECAFPLDSCMNATNHAILQSLVHLMKPDVVPKACCAPTKLSATSVLYYDSSNNVILRKHRNMVVKACGCS"
  },
  {
    "reference": "MISNSINVTPNKESKMYVVKRDGTKENVSFDKITSRISFLCEMEPKLNSDIVDPIEVAQKVVSGVFPGVKTTELDNLAAETAAYMSTRHPDYGILAARISVSNLHKQTSKSFVETVKKQYEFINPKSNLLAPLVSKELYEIVMKHGERLESVIDYYRDFDYDFFGFKTLERSYLHRINGKIVERPQHMLMRVSIGIHGEDLESAINTYKRLSEKLFTHATPTLFNAGTPSPQMSSCFLVQMKEDSIDGIYDTLKQCALISKSAGGIGIAVHKIRAAQSYIRGTNGTSNGLVPMLRVFNDTARYVDQGGGKRKGAFAVYLEPWHADVFEFIDLKKNTGKEEMRARDLFYALWVSDIFMERVEQNGDWALFCPNEAPGLAETHSEEHRALYLKYEATPDLPRRVIKAQELWFAIMESQVETGTPFILYKDACNAKSNQKNLGTISSSNLCTEIIQYTSPDEIAVCNLASIALPKFVRQKVGSTDPKEKEFDHQFLFEITKLITVNLNVIIDRNYYPVAEAKTSNLRHRPIGIGIQGLADVFIKMRMPFDSVAAAKLNVEIAETIYFAALTASHELAIRDGTYESFKGSPASKGILQFDMWNVTPSSRWNWAELKDNIVNKGGLRNSLLIAPMPTASTSQILGNNECFEPYTSNIYSRRVLAGEFTIVNKQLLEDLMELGIWSPEMKNQIVAARGSIQSIGGIPDDLKELYKTVWEIRQRTLIDMAADRGAFIDQSQSFNVFIAEPTFAKLTSMHFYSWKKGLKTGMYYLR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Cysteine radical intermediate active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. Target a Polar residues compositional bias in the protein's composition for improved properties.3. Ideal protein should have a binding affinity for key enzymes in the DNA replication, Genetic information processing pathway to enhance the overall efficiency of the pathway.4. A protein that can perform ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor in ribonucleoside-diphosphate reductase complex to support sorocarp morphogenesis, deoxyribonucleotide biosynthetic process.5. The protein should have a high affinity for dTTP, GDP. The designed protein sequence is ",
    "response#1": "ESGLDFFAYFFKILASQLVSCATPVMRSAGLRQSYLASCFIMNPDMSTEDKTLSAVFRDLSPLLCSKSGVGMNLTNFSSGGKNVQSCLRLINSQVEFCNDKNLRPVSVAAYMELWHEQIEEFLAAKLPENPERCQSIFQGVCVPGLFFRLYEQNPDSQWHLFSPKVGGHLAGLYGDKFDEEYARLVRHGLYSSSLPAKSLMFALISAIIKTGSPYILSKDAINRHHWFETQGNAISYANLCAEVVQQPHEFTSTCNLANVCLPACLRARGEGEGAGAAGEGEGADRLSPLTPDLEFCFDTLRAAVRAAVYMINASILGGVCPTPGVRVMQAERSAGIGVQGLADVFAKLGRGYMDAESALLDARIFEVMYYQAVRASNQLVTVGGAPPHANWKNSKLSRGEFHWESWGVQYDSLFIERQLWEELRESVTKHGTFNSQFIALMPTAGTSQLTGLSESFYPFYANVSSKVSNKEEVMKPNITFLERVSPSDVPLLKYHGGDVSKLPPPLAAKYRNFLTAFDYSPEDQIRRAAARSPFVDQSQSFSFFLKEANVKSASYVKNLILLGHGLGLKTIMYYCRIYD",
    "response#2": "YAAVKQLEGKYLIQNRVNGKIYESAQFLYILISACLFSQYPKNVRMNYIHRFYNAISTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLNSINATTSSIVKYVSQRAGIGVNAGQIRALGSPIRNGEAFHTGCIPFYKHFQSAVKSCSQGGVRGGAATIFYPIWHLEVESLLVLKNNRGIEENRVRHIDYAIQINKLMYQRMLSGDQITLFSPSDVPDLYKAFFSDQKKFKKIYLQYEKNKNIRKKTIKALDLFSLMMQERTSTGRIYVQNVDHCNLHSAFNPELSPIRQSNLCLEITLPTKSLNDVHDTDGEIALCTLSAFNLGKIKSLDDFKELSILSVRALDEILDYQNYPVLAAKKSAISRRSLGIGVINFAYYLAKNKVRYSDGSAHNLTHKTFEAMQYYLLEASCELAKEKGACSLFNHTNYYLGKLPIDTYKKYIDDICNEPLHLDWNLLRSKIKKYGLRNSTLSALMPSETSSQISNATNGIEPPRGFISIKVSKDGILRQVVPEYKKLRLQYELLWDIPNNTGYLQLAGIMQKFIDQSISVNTHYDPARFLNNKIPMKQLLYDLLLSYKLGLKTLYYQNTGGCGQLTIK",
    "response#3": "ESGLDFFAYFFKILASQLVSCATPVMRSAGLRQSYLASCFIMNPDMSTEDKTLSAVFRDLSPLLCSKSGVGMNLTNFSSGGKNVQSCLRLINSQVEFCNDKNLRPVSVAAYMELWHEQIEEFLAAKLPENPERCQSIFQGVCVPGLFFRLYEQNPDSQWHLFSPKVGGHLAGLYGDKFDEEYARLVRHGLYSSSLPAKSLMFALISAIIKTGSPYILSKDAINRHHWFETQGNAISYANLCAEVVQQPHEFTSTCNLANVCLPACLRARGEGEGAGAAGEGEGADRLSPLTPDLEFCFDTLRAAVRAAVYMINASILGGVCPTPGVRVMQAERSAGIGVQGLADVFAKLGRGYMDAESALLDARIFEVMYYQAVRASNQLVTVGGAPPHANWKNSKLSRGEFHWESWGVQYDSLFIERQLWEELRESVTKHGTFNSQFIALMPTAGTSQLTGLSESFYPFYANVSSKVSNKEEVMKPNITFLERVSPSDVPLLKYHGGDVSKLPPPLAAKYRNFLTAFDYSPEDQIRRAAARSPFVDQSQSFSFFLKEANVKSASYVKNLILLGHGLGLKTIMYYCRIY"
  },
  {
    "reference": "MSEPTNLLGLDRSALQDYFASLGEKAFHARQVLQWVYQRGVTNFSEMTDLRLSLRQTLSECAVIDLPEVARTQRSEDGTRKWLLRLADGNCIETVFIPEEDRGTLCISSQAGCMLTCSFCATARQGFSRNLSASEIIGQLLLAEQLCKNDGISSRPVSNVVMMGMGEPLLNYDSVVSAMRLMLDDLSFGLSRRRVTLSTAGVVPAMDRLAEDCPVSLAVSLHAVRDAVRDELVPLNRKYPIAVLLDACRRFLRNDSRRRITFEYVMLAGVNDSLDDARRLVSLLQGIPSKMNLIPFNPVPGVKYSCSDADTIDRFREQLISGGLTTITRKTRGADIAAACGQLAGDVLDRTQRSARLCGASP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.2. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity.3. The ideal protein would be one that plays a critical role in rRNA base methylation through rRNA binding, rRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding, tRNA (adenine-C2-)-methyltransferase activity within cytoplasm.4. The designed protein should have a binding site that is specific to the target S-adenosyl-L-methionine, [4Fe-4S] cluster ligand.5. The designed protein must have a Proton acceptor, S-methylcysteine intermediate active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "ALPNLVGMPRETLKQILADLGIKEKAIKMRTAQIWHWLYIAGVTSFDDMRNVNKDLRNLLAENYSIERPEVVEEQISVDGTRKWLMRMPKQPGDLVGHEVECVYIPEQTRGTLCVSSQVGCTLSCTFCHTGTQRMVRNLTSEEIIAQIMVARDRIGDWPSMDRVNDPNLPQTGRMISNIVYMGMGEPLYNYEHVKNAINIMADGDGISLSRRRITLSTSGVVNFIEKIGEETGCMLAISLHAVTDDVRDILVPINKKFNLETLLEACRNYPGVSNARRVTFEYVMLKDINDSIADAKKLVSLLKGIPAKINLIPFNPWPGSEYECSDWEQIENFAEVINRAGYASPIRTPRGRDIMAACGQLKSESKINRTQVEIMEEE",
    "response#2": "EQRRRTLCVSTQVGCAMGCAFCATGSMGLVRHLAAGEIAGQVEAVRRLLAAEGLERPVSNVVFMGMGEPLHNLEATLAAVEILLHPLGGGMSRRHLTVSTAGLVPAMKKFVERVPAKLAVSLNAADDEVRSRLMPINRRYPLERLLAACRELNLKHTDRITFEYVLLGGINDGDDDARRLVKLLSGLSCKVNLIPYNPLPDSSFRRPRQQRLQRFQQILLDKNLSVFVRRSRGAEVVAACGQLA",
    "response#3": "EKDRGTLCISSQVGCSLACTFCSTGMQGFNRNLSAAEIIGQVMMANLYLQDKGKRISNVVFMGMGEPLLNEKAVYPACDLLLDDLAFGLSRRKVTISTSGVVPALLRMADRIPVSLAVSLHAADDNLRDELVPINQKYPIQELLAACKVYLNAGTQERHILFEYVMLDGVNDSSAQGKKLAILLKGMSAKVNLIPFNPFPESRYKTSKKVTIEKFQDVLFAANIRTTTRSTRGRDIMAACGQLALSAC"
  },
  {
    "reference": "MEQDYKPHEIEEKWQKKWNESLIFQADPDKREKFFITIPYPYLNGNLHAGHTRTFTIGDVVARHKRMLGYNVLYPMGFHVTGTPIVGLAELIASRDPQTMDVYEHLHGIPGDILPTLDTPEKIVDYFKREAEKAMRMIGYSIDWRRKFTTTDPTYKKFIEWQYIRLEEKGLIVKGSHPVKWCPNDNNPVEDHDILYGEEATIVEYTLIKFRYNDLVLPCATLRPETTFGVTNLWVNPDVDYVKARVEKDGNEEFWVVSKEAFRKLTFTDRTVEYVEDVPAKSIIGIKLTNPITGDEVISLPASFVKPENGSGIVMSVPAHAPFDYLALRDLYDADLSEYGITEDLRDIKLISLIQVPEFGEFPAKEIVESMGIANQKAPKAEEATKIVYRREFHGGVLKEITGKYRGYPVSKIKDVLTRDLIASNAGETFYEFSEPVVCRCGTPCVVNMVKGQWFLNYSNPEWKAKVYKCLSQMRIIPEEYRVEFENKVDWLKDKACARRKGLGTRLPFDKEWLIESLGDSTIYMSYYIIARFLERGDLALEQLTLSFFDYVLLGIGDSAAVSAETGLKQELVEEIRSHFNYWYPVDLRSSGKDLVPNHLLFFLFHHVALFEEEKWPRALAVNGFVSLEGQKMSKSKGPILTLESAVSAYGADITRMYILSTAEQTQDADWQKTGIDSARRQVDRFYSFAKDVIESGKRATLSTELKLIDRWMLSRMQKYIMETNIALDSIQTREAIQNSFFLLINDVRWYQRRGGEALLYYVLDNWV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein can be expressed in cytoplasm and perform ATP binding without triggering undesirable cellular responses.2. The protein's functional design necessitates the inclusion of a 'HIGH' region, 'KMSKS' region motif. The designed protein sequence is ",
    "response#1": "DSGAGAGIQADLKVCAARGVYCASVITAVTAQNTRGVQSVHLLPPEFISEQLKSVLSDFEFDVVKTGMLPSTEIVEVLLQNLSDFPVRALVVDPVMVSTSGHVLAGSSILSIFRERLLPIADIITPNVKEASALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGGDLPDSSDSVDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSMLSAVKVAKRFVDNALDYSKDIVIGSV",
    "response#2": "DSGAGAGIQADLKVCAARGVYCASVITAVTAQNTRGVQSVHLLPPEFISEQLKSVLSDFEFDVVKTGMLPSTEIVEVLLQNLSDFPVRALVVDPVMVSTSGHVLAGSSILSIFRERLLPIADIITPNVKEASALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGGDLPDSSDSVDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSMLSAVKVAKRFVDNALDYSKDIVIGSM",
    "response#3": "ISGLTKTFSSASEKLVIFDKLNFSIEEGKKISITGESGSGKSTFLNILGGLESADSGEIIAGSYKVHSLDEKSLTEYRSSFLGLVFQFHYLLKDFTALENVMLPALIAGRSKKEIKEKALSLLEDVKLAERKNHFPSQLSGGERQRVAVARSLINSPSLILADEPTGNLDPANAETVQNLLFSVVDKHKKTLVLVTHDQNIASMTDISYKLYKGND"
  },
  {
    "reference": "MMKFVVFLACLFVAAHSFAVEGEEEYFEAEVPELERAKALPPGSVCDGNESDCKCYGKWHKCRCPWKWHFTGEGPCTCEKGMKHTCITKLHCPNKAEWGLDWRSEESERSPC",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit toxin activity, calcium channel regulator activity.2. The protein must exhibit the following characteristics: This toxin is a blocker of L-type calcium channels (Cav/CACNA1).; They block insect neuromuscular transmission presynaptically.3. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "DWECLPLHSSCDNDCVCCKNHHCHCPYSNVSKLEKWLPEWAKIPDALKRCSCQRNDKDGKINTCDKYL",
    "response#2": "DWECLPLHSSCDNDCVCCKNHHCHCPYSNVSKLEKWLPEWAKIPDALKRCSCQRNDKDGKINTCDKYR",
    "response#3": "DWECLPLHSSCDNDCVCCKNHHCHCPYSNVSKLEKWLPEWAKIPDALKRCSCQRNDKDGKINTCDKYR"
  },
  {
    "reference": "MPTNLLDFDLPQLTAFFAEIGEKPFRAKQVLRWIHHFGESDFARMSDLAKSLREKLSVVAVVEPPRLIKEQLSDDGTRKWLLDVGANNGIETVFIPEDERGTLCISSQVGCALECSFCSTGKQGFNRNLTVAEIIGQLWWANKALGRDPKGERIISNVVMMGMGEPLANFDNVVTALNLMLDDHAYGLSRRRVTVSTSGLVPAMDRLRDTCPVALAVSLHAPNDALRDVLVPINQKYPLAELMAACRRYIEKAPRDFITFEYVMLDGVNDSVAHARELIALVKDVPCKFNLIPFNPFPMSGYNRSRPETVRRFRDELMQAGLIATTRKTRGDDIDAACGQLAGQVQDKTKRTQFNIVESAV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity..2. The designed protein should have a high specificity for S-adenosyl-L-methionine, [4Fe-4S] cluster binding, with minimal off-target effects.3. The protein should have rRNA (adenine-C2-)-methyltransferase activity, tRNA (adenine-C2-)-methyltransferase activity, rRNA binding and be active in cytoplasm.4. The protein's S-methylcysteine intermediate, Proton acceptor must be carefully engineered to ensure that the amino acids involved in adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine are properly positioned for optimal catalysis.5. The protein must bind to [4Fe-4S] cluster in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "SSQVGCALECAFCATARQGFNRNLTVSEIIGQVWHAARELAQPPTNVVMMGMGEPLANFDAVVKAMDIMQDDLAYMLAKQRVTLSTSGIVPNIYRLRAVSDVSLAVSLHAPNDALRDQLVPINQKYPLAELIPACKAYIGHDKRRKITWEYVMLDGVNDRLPLLEGREDLIGLSREQLAGRLTAMGEKSFRAGQVWAWLYNRGIRDIDGMSNISKETRAKMRERFHISRPQVSRELTSADSTRKWLLKYRDGQEAETVYIPDDDRGAVCVSTQVGCTLTCRFCHTGTQMLVRNLSAGEIVGQFMVARDSYGEWPTPNETGRLLSNVVVMGMGEPLFNYDATVTALRILSDPEGLALSKRRITLSTSGVVPRMYDLGNDVGVNLAVSLHAATDDVRDVIMPINKKWPLKELMQACRDYPSASNARRITFEYIMLKGINDSDADARQLARLVKGIPCKFNLIPFNKWPGSDFECSDWSRIERFSDILAEQGYSAPVRKPRGRDILAACGQLKSESQRERLAQRHARQKL",
    "response#2": "QGGRGTLCVSSQAGCALDCSFCSTGKQGFNSDLTAAEVIGQVWIANKSFGTVPAKIDRAITNVVMMGMGEPLLNFDNVVAAMTIMMDDLGYGISKRKVTLSTSGVAPMIDKLGQVIDVSLALSLHAPNDALRNKLVPINRKYPLAVVLDACKRYIGGLGEKRFLTIEYTLLKDVNDQPEHAAEMIALLADVPCKVNLIPFNPFPHSGYERPSNNAIRRFQDLLHKAGHNVTVRTTRGEDIDAACGQLAGEILDRTKRHARFRSVEPIAV",
    "response#3": "TNLLGFDLEGLAAYCEQLGEKRFRATQLFRWIHQKGASDFAQMSDLAKSLRDKLATRAHIQALPVISEQASSDGTIKWLFDVGQGNAVEAVFIPEDDRGTLCVSSQAGCAVGCRFCSTGHQGFSRNLETWEILAQLWFAEHHLRERLNTPDRVISNVVMMGMGEPLQNYAALVPALKVMLDDHGYGLSRRRVTVSTSGVVPMMDRLREDCPVAMAVSLHAPSDPLRDQLVPLNKKYPIAELLAACNRYLDAAPRDFITFEYCMLDQVNDTPEQAEELVRLLKGKVPCKLNLIPFNPFPASGLKRSPKERVQAFAEVLIRAGIVTTVRKTRGDDIDAACGQLAGEVQDRTRALIARARGQVGRSGALCVSSQVGCTLNCTFCHTGTQKLVRNLTAQEIVNQVLIARDELEEWPSSNENRKLTNIVFMGMGEPLYNLDNVAEAIDTISDGDGIALGRRRITVSTAGVAPKIPELGERTGAMLAISLHAVRDDLRNEIVPINKKYDLQTLFEAIRAYPDLGNSKRVTFEYVMLKGVNDSPAEARDLVKLLKGVPAKINLIPFNPWPGSPYECSDWETIETFAEIVNRAGYASPIEASCGQLRNG"
  },
  {
    "reference": "MSMQPTDHIRLTAFVHGHVQGVGFRWWTRSQALELGLAGSATNLDDGRVCVVAEGPEDRCRELLTRLGEQPSRHRRVGTVSTVIEQWSEPRGVEGFTER",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must have at least one Acylphosphatase-like domain.2. The designed protein must be able to perform acylphosphatase activity under specific conditions.3. The protein with the reaction an acyl phosphate + H2O = a carboxylate + H(+) + phosphate, the active site must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MIEGRVQRVGFRASCNRRALDLGISGWVRNLSDGRVEVQAEGPPLALSELRAWCEVGPPGARVVRVTPSQLPITGDDWFEVRR",
    "response#2": "MIEGRVQRVGFRASCNRRALDLGISGWVRNLSDGRVEVQAEGPPLALSELRAWCEVGPPGARVVRVTPSQLPITGDDWFEVRA",
    "response#3": "QVMIRGRVQGVGYRAWVEYQATASGLEGWVRNRRDGSVEALFAGAPNHVADMVALCRHGPPSSRVDSVTSETAC"
  },
  {
    "reference": "MKNIRAVVLAAGLGTRMKTETPKVLHRVGSRTILGRIVSGIKGAGITDITAVIGFKADIVEYFFKDSVRFVRQPELLGSGDALKYAVDGMEDGKGSILVACGDTPLITDETYRKLVRTHLSEKASCTLLTCRVPEPLFYGRIVRGKNGNVLKIVEEKDLSAGERKITEINAGIYCFSEGDIRKFIKDIKINEKKKEFYLTDIVDILARNGRKIVSENCDPDETIGINSRRDLAIANRIVNRRTAERLMGSGVTIIDPDTTYIDETAVIGRDTVIFPDTVIESDVTVAGGCRIGPFARLRPGTSVSEGVEIGNFVEICRTRIGGGTKIKHHTYLGDTVVGKNVNIGAGTITANYDGKNKHRTVIEDNAFVGVGAVLIAPVRVGKGATVGAGSVVTKNKDIPAGETVAGIPAKPLSSR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be optimized for activity in UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA, Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.3. The protein requires Mg(2+) to activate a critical catalytic site.4. The designed protein must have at least one MobA-like NTP transferase domain.5. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc) The designed protein sequence is ",
    "response#1": "MSGVVLAAGQGSRMNSDLPKVLHKVGGAPLFAHALNAGAALEPERSVLIAGHGAEAVRAEASEWDEDLVVALQEQQLGTAHAVAQARDALEGAEGDVFILYGDTPFIRPETLSMMAAARAGGAAVVVLGFEAADPARYGRLVTAGGDLERIVEFKDASEAERAITLCNSGLMAVRSADLFRLLARVGNDNAAGEYYLPDVVGIARAERADLATAEAAFQARARDAALEDGVTLRAPETVFFSYDTVIGRDAEVEPNVVFGPGVTIETGARIRAFSHLEGCHVSRGAIVGPFARLRPGAELSENVHVGNFVEIKNAQVDEGAKINHLTYIGDASIGAATNIGAGTITCNYDGVFKHRTEIGARVFIGSDTMLVAPVTIGDGAMTATGSVVTSDVPAGALAIGRAKISGWVQPERKSRADMASRLMARAKERKAAR",
    "response#2": "VVLAAGQGSRMNSDLPKVLHKVGGAPLFAHALNAGAALEPERSVLIAGHGAEAVRAEASEWDEDLVVALQEQQLGTAHAVAQARDALEGAEGDVFILYGDTPFIRPETLSMMAAARAGGAAVVVLGFEAADPGRYGRLVMDGDRLDRIVEFKDASEEERKVTLCNSGVMAVDGALFLELLRQVRNENAKGEYYLTDVVEIARGAGGRAVAVTGDEAETLGVNSRADLAEAEAAFQARMRAQMMADGVTLIAPETVFFAHDTKIERDVVIEPNVVFGPGVVIEEDVVIHAHSHVEGAHLKRGAHAGPFARLRPGAELGEGSKVGNFVEIKKSQLAEGAKVSHLTYIGDASIGANANIGAGTITCNYDGYDKHRTVIGENAFIGSNTCLVAPVTVGAGAFTATGTIVTEDVPADALALARTPQMVKPGWRERFHALMAARKKKTKD",
    "response#3": "VVVLAAGEGKRMKSALPKVLHPLLGRTLLGHVLTAAEPLAADRTVVVVGHGADQVRAHLTEVAPQADAVLQAEQLGTGHAVRIALDAVADAAGTVVVINGDVPLLRPETVAALVEAHEGAGAAATVLAAEVPDPTGLGRIVRDAAGRLERIVEEKDADDAQLAIREINSGIMALPGKRLRDWLARLDNRNAQGEYYLTDVVAMAAADGVAVRTAQPETEIEVEGANNRVQLAALERAFQARQAETLMLEGVALADTDRIDIRGNLKCGRDVFIDVGCIFEGNVELEDGVHIGPYCVLKDVKIGKDSKVEAYSHLDQAELATQCKVGPYARLRPGAQLGNGCQIGNFVEIKNTQMGDFSKASHLSYLGDALIGKSVNIGAGTITCNYDGANKFVTTIGDNAFIGSDTQLIAPVTVGEGATIGAGTSLRQDAPAHQLTLTQTSQRTIRGWKRPVKAPKK"
  },
  {
    "reference": "MSVNRPAAVIVLAAGEGTRMKSQLPKVLHEIGGRSLVGHVLAASRELDPLHTVVVLGHARERIAEHLALIAPEAVTAVQEEQKGTGHAVRMALEDLARQGIEPTGTVVLTCGDTPLLRGATLSELVSFHEKEGNAVTVLSARVPDPHGYGRIVRDADGGVTAIVEQADGTPEELAVDEINSGMYAFDGALLAQVVHRIGSDNAKGEEYLTDALALLRGDGHRVGAWAAEDADEVQGVNNRVHLAQARRLLNDRLLHGHMLAGVTVVDPATTWVDVGVRIGRDTLVEPGTRLRGTTSVGEDAVVGPDSDLTDTVVADGAKVRRTTADRAEIGPGADVGPYTYLRPGTRLADRTKAGAFVEVKNANIGSGSKVPHLTYVGDADIGVGSNIGCSSVFVNYDGVDKSRSTIGDHVRIGSDNTIVAPVTVGDGAYSGAGTVVRDDVPPGALAVSEGHRQRNVEGWTARKRPGTASAEAAHRARPAREAQGVEDAERHRAGDQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The presence of Mg(2+) is necessary for the protein to function in the desired environment.2. The protein design should emphasize Basic and acidic residues compositional bias.3. The UDP-N-acetyl-alpha-D-glucosamine binding site should be stable and able to withstand changes in environmental conditions.4. The designed protein must possess magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity to support regulation of cell shape, cell wall organization, cell morphogenesis under certain conditions.5. The protein design should take into account the physiological conditions of the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway.6. The protein should be designed to have high stability and activity towards acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.7. The designed protein must have at least one MobA-like NTP transferase domain.8. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The designed protein sequence is ",
    "response#1": "MNAVILAAGESTRMKSSMSKVLHPVAGRSMIAHVVEAVAGAGVGTVALVVGRDAENVAKAASLPGVSVESFLQKERKGTGNAVLAARDAIARGFDDVIVAYGDVPLITSATLNRAREALNTGVDVVVIGFHTDRPTGYGRLLTEPDGSLVRIVEERDATPEERAIRLCNSGVICADADLLWQGIEAVRNDNAQKEYYLTDIVELARGDGRLGVTLADPARFDLRGVLECGRDVFIDAGCVFEGRVELADGVRVGPHCVVKDARIGAGTHIEAFSHIQDAVVGEGARIGPYARLRPGADIGDQAHVGNFVEIKKSRLGKGAKANHLAYIGDARIGARVNIGAGTITCNYDGVNKFQTIIEDDAFIGSDSQLVAPVTVGRGATLGAGTTLTKDAPAGQLTISRTVQEETRGWRDRNRP",
    "response#2": "MKNAIILAAGKGKRMKSTLPKVLHKVCGKEMVNHVIDTMRASDLSDINIVIGNGSDDVKESTKFTDVTYSFQDKQLGTGHAIMCAKEFLKHKKGIVAIFNGDVPLMREDTVRRLINTHQDNGYQCTVATSILEDPTGYGRIIRKDGLVDRIVEHKDCNKEQLNTKEINAGIYCFDIESLVKGLDKLTNNNAQGEYYLTDIVEILKDNGKTVGAMSEDFEETLGVNDRIALSRAEKVMRKRINEKHMRNGVTLADPARFDLRGVLECGRDVFIDAGCVFEGRVELADGVRVGPHCVVKDARIGAGTHIEAFSHIQDAVVGEGARIGPYARLRPGADIGDQAHVGNFVEIKKSRLGKGAKANHLAYIGDARIGARVNIGAGTITCNYDGVNKFQTIIEDDAFIGSDSQLVAPVTVGRGATLGAGTTLTKDAPAGQLTISRTVKDERTIEGWVKRKNKK",
    "response#3": "MILILAAGQGMRMESSIPKVLHHVGGRPMLFYILRIANALKPGAIGVVVGHESDQVKAATLEMARSVGITRAICFITQKKPLGSGNAVVESLPFLKKFKTAAVMCGDTPLLTYESIYNLLLTHDQLKGQATLLTARIANPKGYGRIIRGALGEVQRVVEESVATSKEAAVNEVNSGAYVFELEALISGLKKIGAKGPKKEHFLTDVMEAIRAKGRITGFVEHKDAEEIMGINDRVQLSEVENILRKRKNYELMKEGVTLADPARFDLRGVLECGRDVFIDAGCVFEGRVELADGVRVGPHCVVKDARIGAGTHIEAFSHIQDAVVGEGARIGPYARLRPGADIGDQAHVGNFVEIKKSRLGKGAKANHLAYIGDARIGARVNIGAGTITCNYDGVNKFQTIIEDDAFIGSDSQLVAPVTVGRGATLGAGTTLTKDAPAGQLTISRTVGVTMLDPDQVWVGPDARIGRDCVLLPQTIIWGRTTVGEACTIGPNSRLVNASVGDRCLVDETIIVDSAIDDDVSCGPRAYLRGGAHFMRRSKAGTHVEIKGSEVGEGSKVPHLSYIGDARLGRGVNIGGGSITCNYDGKHKSRTEIGDHVFVGSDTMMVAPVEIGDNALVGAGSCITQDVPAGALSLERSRQKNIPGWVKKK"
  },
  {
    "reference": "MRVRGTQRNWQQLWIWGILGFWMVMICNVWGNRWVTVYYGVPVWKEAKTTLFCASDAKAYEREVHNVWATHACVPTDPNPQEIVLENVTESFNMWENDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCSNTTSNTNITNNGEMKNCSFNVTTEMRDRKQSVYALFYELDVVSVNEGNSSFYRLINCNTSAITQACPKVSFDPIPIHYCAPAGYAILKCNNETFNGTGPCNNVSTVQCTHEIKPVVSTQLLLNGSLAEKEIIIRSENLTNNVKTIIVHLNESVEIVCIRPNNNTRKSVRIGPGQTFYATDIIGDIRQAHCNISRSQWEKTLQQVEGKLTEHFPNKTIRFEPSSGGDLEITTHSFNCKGEFFYCNTSGLFNGTYNSSSTGNNDESITLQCRIKQIINMWQEVGRAIYAPPIAGSLTCRSNITGLLLVRDGGNNSDTNGTEIFRPGGGNIKDNWRSELYKYKVVEIKPLGVAPTEAKRRVVEREKRAIGAVFLGFLGAAGSTMGAASIALTVHTRKLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGLWGCSGKLICTAAVPWNSSWSNKTTKEIWDNMTWMQWEREIDNYTNTIYELLEVSQNQQEQNEKELLALDNWKNLWNWFDITNWLWYIKIFIIIIGGLIGLRIIFGVLSIVNRVRQGYSPLSFQTLPPSPRGLDRLGRIEEGGGEQDRDRSIRLVNGFLALAWEDLRNLCLFSYHRLRDFILVTARTVELLG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.2. The designed protein should contain one or more Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains that are essential for its function.3. A protein that localizes to host cell plasma membrane, host cell endosome membrane and can perform structural molecule activity with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAIERYLRDQQLLGLWGCSGKLICTTTVPWNSSWSNKSYTDIWNNMTWMQWDREVSNYTGIIYRLLEDSQTQQEQNEKDLLALDSWKNLWSWFDISYWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTY",
    "response#2": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAIERYLRDQQLLGLWGCSGKLICTTTVPWNSSWSNKSYTDIWNNMTWMQWDREVSNYTGIIYRLLEDSQTQQEQNEKDLLALDSWKNLWSWFDISYWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTR",
    "response#3": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAIERYLRDQQLLGLWGCSGKLICTTTVPWNSSWSNKSYTDIWNNMTWMQWDREVSNYTGIIYRLLEDSQTQQEQNEKDLLALDSWKNLWSWFDISYWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTR"
  },
  {
    "reference": "MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQILTMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFAILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARRWLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a specific function in transmembrane transport and should be localized to plasma membrane in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "MGVSIFITVVGTAVQLFFTFTMAYPLAKRHVKGRNLLLNLVIFSMLFSGGMIPTYLVVKSLGLLDTYWALILPMAINPFNLIIIKNFFQQLPRELEESAKIDGCSEIGVFWRIALPLSKPVIATFALFYAVGIWNDFFHALLYINDSAKWPLQMVLRQVTILSDLTATNGDTMQNAVPPEQGIKLAVIVIATLPILAVYPFF",
    "response#2": "QSFWLITGIIFIAFNLRPAITSVGPVISSIRAELHMSNGAAGFLTALPLLSFAVLSPLAPKLGQRLGNERTLWLGLVILLIGVLTRSTGYTAALFFGTALIGVGIAIGNVLLPSLIKHKYPEKPGIMISLYTTSMNIFAALASGVSVPLATQMNGGWKQAFLLWGGLALLALLIWIPQLRHRDTANQTMKLQSSSIWASKMAWYVTIFMGLQSFLFYSSIAWFPEILRSHGIDTATAGWMVSLMQFASLPSTFLTPVLADRVKQQRGIVAALASVYLIGLCGLLAGGSHTLLAIWMIIIGIGQGSSISLALTLIGLRSENAQQAAALSGMSQSFGYLLAAVGPIFVGYLFDQTHSWTMPIVLLIAALIVMGAAGQGAGRDRYR",
    "response#3": "MGVSIFITVVGTAVQLFFTFTMAYPLAKRHVKGRNLLLNLVIFSMLFSGGMIPTYLVVKSLGLLDTYWALILPMAINPFNLIIIKNFFQQLPRELEESAKIDGCSEIGVFWRIALPLSKPVIATFALFYAVGIWNDFFHALLYINDSAKWPLQMVLRQVTILSDLTATNGDTMQNAVPPEQGIKLAVIVIATLPILAVYPFL"
  },
  {
    "reference": "MNLNDLLSDFFYIKEQNIFIQNMCINSKKILNNCLFVALKGHLIDGQDYINDAILNGAVAVLTYNTDRYNNYVTYINLIPVIHIKNLQYHLSSIAHKLYKSKNSNIPIIGVTGTNGKTSVTNILMQWLYLLKQNPTICSTIGNGFYNSLEYTNNTTDSTIDIQYNINKFIQKKSNVIILEVSSHGIKQHRVRNINFTTAIFTNLSRDHLDYHKDMRDYANTKWSFISQYSINNVIMNIDDDIGLQWSKQLPNAILVTTKQNLINKQKKYFLVKNINFKTDTTIIQFETTWGAGKISINLLGYFNVINIILSLTTLLSLNYTLEDLLYTSYKLKPVYGRFNIIQKNNCPKIIIDYAHTPDALKSILQTIKFYYKSTIWCIFGCGGERDQGKRSLMGIIASSLANYIIITSDNPRNENINDIIKDIIQQCSFKKKYMSIIIDRKEAIQYALTKAHKQDVILIAGKGHEQYQIIGHKHYVYSDYDIVNDYYKQK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a binding site that is specific to the target UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate ligand.2. The protein requires Mg(2+) to activate a critical catalytic site.3. The designed protein should contain at least one Meso-diaminopimelate recognition motif motif to achieve its intended function.4. The designed protein should contain one or more Mur ligase C-terminal, Mur ligase central, Mur ligase N-terminal catalytic domains that are essential for its function.5. The designed protein should interact with enzymes and substrates within the Cell wall biogenesis, peptidoglycan biosynthesis pathway to enhance or regulate its activity.6. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.7. The protein have enhanced ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "VTGTSGKTSVAAFTRQIWQANGHASASLGTLGVVAPAGAVYGSLTTPDPIELHHTLDTLVSEGVSHLCLEASSHGLDQHRLDGVRISAGAFTNLSRDHLDYHPTMEAYLAAKMRLFSELVPRGGGAAVWVDTDEGSHAAEIAAHHSLEVLGIGITGAGIALLERTDEGLGQRLLVEVAGKQRSILLPLVGAFQAANALVAAGLALGMGVAKDSLDVLVTGTNGKTTVTVMLEAIARAAGRSCGKIGTLGVTLDGVDEVLSHTTPEGADLQRLLRRMVDRGVSMVAMEVSSHALALSRVEGVSFSVAAFTNLSQDHLDFHGNMEDYYLAKASLFDGRAAVHVIDVTTAAGRRLARSATGPVVTVGRGPGHDVSIVSPDPDLSQARFRCRIGGQDVALDVRPGGLHNIHNAAVAAACAGLRALGVPLAQIAEGRMQSICCGQPFGVYVDDARTPDALAVTLKALRNVTAGRVICVFGADAAADPANRPLMGRIVERTAHLGIITNDNPRDQSPLQIAHDIIDGYDRPARSHILPDRGEAIRWALAEAEPGDAVLIAGKGDVTQTLMLVQEARA",
    "response#2": "MSLISGVTHASGEVVPGDVYAALPGSRRHGAEFVAEAVAAGAVAVFTDAAGAPAATAAGVPALVVDDPRARLGELAAEVAARIYGEPSSRLQLVGVTGTNGKTTTAFLVHRIMKQTMQRAGLVGTVVSDDGMTQEQADFTTPGPISLQSLLARIRNNGCPGVALEVSSQGLDQRRADAITFDVAVFTNLTRDHLDYHGSMEKYFASKRRLFENAAESSAGKNPVAVINGDDPYGEALIREFSERMNVLSYGFSVESDFRAGNVQQTAQETRFQLFAKGKSYLVRLPLIGRFNVYNALGALTGALALGLEPDQIAAGLAAVPGVPGRLELVGTRNGAGVFVDYAHKPDALATALAALRPYVTGRLIVVFGCGGDRDRGKRPIMGRIAGDKADLVIVTDDNPRSENPAMIRKEILAAVPGAREIGDRAEAIRAGIGLLQTGDVLLIAGKGHEDYQIIGTTKHHFDDREVAREALAARAGGR",
    "response#3": "MKLSTLLSRLQDLNLLSPDSRRCAPGVAFFAFPGDAADGRAHIPEALARGAAAVLWEEDGFSWRQEWRVPNAAVRGLRQQAGRLAHEFAGSAWDDLPTLLVSDPRQAMGQLAARFYGHPTQTLQTVGIIGTNGKTTVTHLLAQAWLALGITGTNGKTSTAWWLAEALSRSERIAQSPCGVIGTLGIGMPPALVSTGLTTPDPVMLQRELRAFVSRGFGACAIEASSIGIAERRLDGTRIAVAVFTNFTQDHLDYHGDMDAYWQAKAELFRWPGLRAAVVNLDDVHGASLVGNLVESGVGSLDVWTVSAAGSPARLRATDIGYGVDGLEFSIVESSLPVERLATQLIGQYNVDNLLGVVGRMQRVGGAGEPLVVIDYAHTPDALEKVLQALRPVAVARGGRLVAVFGAGGDRDRSKRSPMGAVAARLADRVVLTSDNPRSEDPRAILEAIRVGVPGPCELEPDRARAIERALTQAAAADVLLLAGKPPAHLELITTVCAVTGTKGKTSTTYLIKAVLEAAGEKVGLIGTIRNLIGDEILPAHRTTPESADLQSLLRRMADRGVDSVVMEVSSHALSLHRVADVSFDVGVFTNIGRDHLDFHQSFHEYLEAKKLLFRSLARGREATGGVGKGTRFAVINRDSPVWHEVAQAAAGVPIYTFGIDSKEADVQAVAIASELGIDAGQAAAALASARPPAGRFDYMVSDSQVTGIVDYAHTPDALENVLNTIQQIRNPKQKVITLIGCGGNRDAAKRPVMADIACRLSDKVILTSDNPRFEEPQAILQDMQKGVKPLDYKKTLSVLDRKEAIKTACMLAEPNDIVLVAGK"
  },
  {
    "reference": "MNTSQPALHTSMLTSLPLLARGKVRDNYAVGIDRILMVASDRLSAFDVIMGEPIPGKGALLTQMALFWFDKLGPKGLNLCPIHLTGDAPESVVTAAEVPQVTGRSMLVKRLKPLPVEAVVRGYLAGSGWKEYQHNGAVCGVKLPAGLQNASKLPEPIYTPAAKAAAGDHDENITFEQTVEMIGLDLATRIRDISIAIYKAASEIARAKGIIIADTKFEFGLDADGTLTLMDEVLTPDSSRFWPAESYQEGINPPSFDKQFVRDWLEQVQVNGKPWNKTPPAPRVPDEVIAKTAAKYQEALTRLIG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that is capable of catalyzing reactions within the 5- amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5- phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2, IMP biosynthesis via de novo pathway, Purine metabolism pathway with high efficiency and specificity.2. A protein with ATP binding is needed to enhance 'de novo' IMP biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "LYTGKAKSVYRTDDPEVYIMKFRDDITAFDGEKKDTLEGKGRYNAEVSTFIFRYLEEHGIRTHYLASIESGVIAVRNLTMIPLEVIVRNVAAGSIVRNYPFREGEPLDPPLIVIDYKSDAHHDPMLNDELIYALGLATPEELDQIKAMALAINELLSDYLDLRGITLVDFKMEFGRYNGEIVVGDEISMDSMRLWDKETGTSLDKDVYRFGKGDVMETYAGVAKKTFIQSLDIPTAQFQSVESFNDLKSAIEKIGYPAILKSNTMGYDGKGQVKLTDQVDLEQAWKKVTSETCATKAILEQYIEFESEASVIVARALDGTELTFPLVTNKHRNHILRQTIAPAQLPEYIHKQANEIGLKIARSNGLVGIIAVELFVVKNNETGQYSLMVNELAPRPHNSGHWTIEGCVTSQFEQLIRCVE",
    "response#2": "LYTGKAKSVYRTDDPEVYIMKFRDDITAFDGEKKDTLEGKGRYNAEVSTFIFRYLEEHGIRTHYLASIESGVIAVRNLTMIPLEVIVRNVAAGSIVRNYPFREGEPLDPPLIVIDYKSDAHHDPMLNDELIYALGLATPEELDQIKAMALAINELLSDYLDLRGITLVDFKMEFGRYNGEIVVGDEISMDSMRLWDKETGTSLDKDVYRFGKGDVMETYAGVAKL",
    "response#3": "LYTGKAKSVYRTDDPEVYIMKFRDDITAFDGEKKDTLEGKGRYNAEVSTFIFRYLEEHGIRTHYLASIESGVIAVRNLTMIPLEVIVRNVAAGSIVRNYPFREGEPLDPPLIVIDYKSDAHHDPMLNDELIYALGLATPEELDQIKAMALAINELLSDYLDLRGITLVDFKMEFGRYNGEIVVGDEISMDSMRLWDKETGTSLDKDVYRFGKGDVMETYAGVAKL"
  },
  {
    "reference": "MRVTGIRKSYRHLWRWGTMLLGMLMICSAVGNLWVTKYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPSPQEMFLENVTENFNMWKNEMVNQMHEDVISLWDQSLQPCVKLTPLCVTLECRNASSNGRYSEEMKSCSFNTTTVVRDRQERVEALFYRLDIVPLPEKNSSKNSSEYYRLINCNTSAITQACPKVSFNPIPIHYCTPAGYAILKCNDKEFNGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIIRSENLTNNVKTIIVHLNKSIEIVCTRPNNNTRKSIRIGPGQTFYATGEIIGNIRQAHCNINRDKWNKTLQLVSRKLAEHFQNKTIKFASSSGGDLEITTHSFNCRGEFFYCNTSGIFNSTYMPNGTYIPGTKGNSTITIPCKIKQIINMWQEVGRAMYAPSHLQENITCKSDITGLLLVRDGGTDDANDTEIFRPGGGDMKDNWRSELYKYKVVEIKPLGVAPTEAKRRVVEREKRAVGIGAVFLGFLGVAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKSQEDIWNNMTWMQWDKEISNYTDTIYRLLEDSQNQQERNEKDLLALDSWKNLWNWFDITKWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTLIPNPGGPDRLGRIEEEGGEQDKGRSIRLVNGFLALVWDDLRNLCLFSYHRLRDFIL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit structural molecule activity. The designed protein sequence is ",
    "response#1": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKIICTTAVPWNSTWSNKSYEEIWDNMTWAEWEREISNYTNQIYDILTESQNQQDRNEKDLLELDKWASLWNWFDITNWLWYIKLFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTPS",
    "response#2": "LQVFRKMLTPRIELSVWKDDLTTHAVDAVVNAANEDLLHGGGLALALVKAGGFEIQEESKQFVARYGKVSAGEIAVTGAGRLPCKQIIHAVGPRWMEWDKQGCTGKLQRAIVSILNYVIYKNTHIKTVAIPALSSGIFQFPLNLCTKTIVETIRVSLQGKPMMSNLKEIHLVSNEDPTVAAFKAASEFIL",
    "response#3": "GTTAAPVSISRNINRRSKPSIKMMGDAVVISHSEMLGAINSGTPSSNVTAFRCTGYRANPGMSTIFPWLSATAVNYEKYKFRRLSFTLVPLVSTNYSGRIGVGFDYDSSDLVPGNRQEFYALSNHCENMPWQESTVEIKCDNAYRFTGTHVAADNKLIDLGQVVVMSDSVSNGGTISAALPLFDLIVNYTVELIEPQT"
  },
  {
    "reference": "MPRSLKKGPFVDDHLLKKVDALNESGKKTVIKTWSRRSTIIPDMLGHTFAVHDGRKHIPVFVNESMVGHKLGEFAPTRTFKGHVKDDRKSRRR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have rRNA binding, structural constituent of ribosome and be active in cytoplasm. The designed protein sequence is ",
    "response#1": "GFAEGDKVDVTAISKGKGFQGAIKL",
    "response#2": "GFGK",
    "response#3": "ML"
  },
  {
    "reference": "MKIVKQDKKEGIVTLVPETLDDLWHLSHIVEVGDSVSSKTTRRIQDNTGDKLRSDRGVKKTFTLRIDVENITFHIFTGKLRLTGVITKGPEDLIPLGSHHTVEVKLNTPITIKKEKWANWALKRLNQAIEASKKLAAIIVLLEDDTATLGLMRQFGIEYYGPIKGSVSGKRIVDKNRSKAIAQFYEKVIESVNKFHDIQNIVVAGPGFVKNDFYDYIKNKHKDLADKAIIESTGSGGRVGIHEVLKKGTVEKLTVENRVASEMVAINNLLEEIGKNSSKVAYGEKETVKAINLGAVKQLLVLDSAVAINDMGNLMDMVENMNGEVMVISSQHEGGEQLKGLGSMAAILRYEIA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA; May play a role in the release non-functional ribosomes and degradation of damaged mRNAs; Has endoribonuclease activity.2. A protein that can perform endonuclease activity, metal ion binding in cytoplasm to support RNA surveillance, nuclear-transcribed mRNA catabolic process, no-go decay, nuclear-transcribed mRNA catabolic process, non-stop decay. The designed protein sequence is ",
    "response#1": "MRNNSSDVDAKLTSFMEEAQNSTNSEEMLGEIVRTIYQKAVSDRSFAFTAAKLCDKMALFMVEGTKFRSLLLNMLQKDFTVREELQQQDVERWLGFITFLCEVFGTMRSSTGEPFRVLVCPIYTCLRELLQSQDVKEDAVLCCSMELQSTGRLLEEQLPEMMTELLASARDKMLCPSESMLTRSLLLEVIELHAR",
    "response#2": "MRNNSSDVDAKLTSFMEEAQNSTNSEEMLGEIVRTIYQKAVSDRSFAFTAAKLCDKMALFMVEGTKFRSLLLNMLQKDFTVREELQQQDVERWLGFITFLCEVFGTMRSSTGEPFRVLVCPIYTCLRELLQSQDVKEDAVLCCSMELQSTGRLLEEQLPEMMTELLASARDKMLCPSESMLTRSLLLEVIELHAQ",
    "response#3": "MRNNSSDVDAKLTSFMEEAQNSTNSEEMLGEIVRTIYQKAVSDRSFAFTAAKLCDKMALFMVEGTKFRSLLLNMLQKDFTVREELQQQDVERWLGFITFLCEVFGTMRSSTGEPFRVLVCPIYTCLRELLQSQDVKEDAVLCCSMELQSTGRLLEEQLPEMMTELLASARDKMLCPSESMLTRSLLLEVIELHAQ"
  },
  {
    "reference": "MSRVLLIVSGGIAAYRALDVARLLRQQGMDVTPVMTEAAKEFITPLSLEVLCAELVHETLFEPAQESTIGHIALARAADLVLVCPATAHIMARMAQGLADDLATTLLLATTAPILLAPAMNWRMWEHPATQQNLALLRQRGVHLVGPDEGSMACGEQGVGRLSTPEAIVASVQHCLNPPQSLRGKRVLVTAGPTVEPLDPVRFLSNHSSGLQGYAVAEALAYYGAEVTLVSGPVSLPTPPRVTRIDVQTAVEMAQACEALLPVDVAVCVAAVSDWRPRSQATEKMKKQGDQPPTFELVENPDILATLSHHEQRPPLVIGFAAETENVLDHARAKRLRKGCDWLLANDVSQYAFGGQTNQVYLFDQEGYETWPVMDKKVLAMRLADKVSRYLTSDLFKE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A The designed protein sequence is ",
    "response#1": "SVLITAGRTEEAIDPVRYISNRSSGKTAVALANEFLKAGYRVFFVHGPMDVKVPFGVFDVPVTSSQDMYDRVLSAQSKMDVIVHCAAVADYRPKHVSETKIKDSRGQLVLELEPNPNILRDSVKNKAAKQVIVGFGLETDDVQKHGEEKLSKSGADFLVLNTPVVDGAGFGYDR",
    "response#2": "MRIVLGVGGGIAAYKVASLLRLFTEAGHDVTVIPTEAATRFVGVATWEALSGNPVTNSVFDDVDQVKHVRLGHEADLIVVAPATADLLARAATGQANDLLTNTLLMASGPVLFAPAMHTEMWQHAATQANVETLRSRGVTVLEPASGRLTGSDSGPGRLPEPEAIFDAALERV",
    "response#3": "LVCVTGCIAAYKACELVRELQRRGARAKALMSEHATHFVDPTTFRALTREPVALGLFDDPSDPIHHISLAKEADVIIVAPATANVIAKMAAGIADDLLSTTLLAATSPIVVAPAMNANMWRAQVTQENMARLRERGIAQVGPGSGYLACGDVDEGRLIL"
  },
  {
    "reference": "MEPIAVVGISCTFAGGINTSELFWKVLKDSIDVGSEIPKERFDPDSYAPLYNTKKPLIRRGYFINNDELDHFDPSFFGVTDADAMSMDPCHRILLEKFVHLLEDANYPVDQMKGSRTAVYIGQFTTDHPTTFYRSKVEDQSNLLGPNISVYNASARLSYHFDLHGPNLTLDTACCSSLQALHLAVQSLRNGEADFAVAGGTNLNYAPESFFISLITGAISPDGRSRSYSEDANGYARGDGVAMVLLKRLSDAIRDKDKIYCVVRDVMASHDGNEEKMNYNVPSSFGQNLLLKEIYSRNNIDLNEVFYIEGHGTGTQVGDPIEANTLGKFFKRSPYNPPLLIGSVKSVIGHTEATAGIASLIKIALCMKYRMLTPNMNFTSLNPKIEAEKYNLHVVNHIINFPEQTVTVGINNFGMGGNTAHAIVSEWVEDHSYNSSIHIDEKLEQDFILTFSSKCDQSLKIHLEKISKWLSTIPKQFIENHEQLFLAYLSQKLLLKRTTNFSHRLSFVCSNFKQLEHQIQSYLADQTTTPGVVLPQDKLLIEHQSNSNICFIYSGQGPQWWAMGRQLYLTESIFRHWIDKLHLEFLSVSNHSFSLIKELIEPTKEEDSQINFTNIAQPLILSVQIALTSLWLSWGIRPQYIVGHSVGEVAAAYVAGRLTLNEAVQIIYHRSRVQNFNTNQGGKMLALFLSEDETNKLLINCQDRIQIAAISSPKSVTLSGDKDALENLYNNLSITQPNVYKTWLKIENAFHSKQMERFHIYEELIESL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Ideal protein should have a binding affinity for key enzymes in the Lipid metabolism, fatty acid biosynthesis pathway to enhance the overall efficiency of the pathway.2. The presence of Carrier domains is critical for the interaction of the designed protein with its intended targets.3. We are looking for a protein with broad substrate specificity for dodecanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxotetradecanoyl-   [ACP] + CO2 + holo-[ACP].4. The protein must have high specificity towards oxidoreductase activity, phosphopantetheine binding.5. For general function, the protein need meet that This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain. The designed protein sequence is ",
    "response#1": "MEDIVIAGISGRLPESNNLEEFWQNLFNGVDMVTEDDRRWKPGLYGLPRRNGKLKEIDRFDAAFFGVHPKQAHTMDPQLRLMLEISYEAIVDGGINPVSMRGSKTGVYIGVSGSEAGEAFSKDPEELLGYSMTGCQRAMFANRLSYFFDFNGPSTAIDTACSSSLLALENAFNAIRHGKCDAALIGGVNLLLKPNTSVQFMKLGMLSPEGTCKSFDASGNGYCRSEAAVAVLLTKKSMAKRIYATVLNAGNNTDGYKEQGVTFPSGEMQQRLVRSLYQEANISPEQVEYIEAHGTGTKVGDPQEVNGIVSVFCQSQRDPLLIGSTKSNMGHPEPASGLAALAKVVLSLEHGVWAPNVHFNEPNPDIPALTDGRLCVVSKPTPVRGGIVGINSFGFGGSNVHVILSSNPKPQPRVSVFTGMGSQWPGMGKSLMKIPIFSAAIEKCQTALRPHGIDVINIITSSDPTIFDNILHCFVGIAACQIGLVDVMKAIGIEPDGIVGHSVGELGCSYMDGSFTAEQMILAAYYRGRASIETKLVKGTMAAVGLGAQKAKEICPPDIAVACHNGPDVCTLSGPAESITKFVKTLQQKGIFAREVNCANIAYHSKYISSCGPTLLKYLKQVIPNPKPRSSRWVSSSIPESAWETPLATHSSAEYHRWAERTGGMAFHSYFMDAIAPTLLQQLKKVIRDPQPRSSRWLSTSIPKTQWQESLARTFSAEYNVNNLVSPVLFQEALWQVPENAVVLEIAPHALLQAVLKRGLKTSCTIIPLMKKDHKDNLEFFLSSMGRLYLTGID",
    "response#2": "MEDIVIAGISGRLPESNNLEEFWQNLFNGVDMVTEDDRRWKPGLYGLPRRNGKLKEIDRFDAAFFGVHPKQAHTMDPQLRLMLEISYEAIVDGGINPVSMRGSKTGVYIGVSGSEAGEAFSKDPEELLGYSMTGCQRAMFANRLSYFFDFNGPSTAIDTACSSSLLALENAFNAIRHGKCDAALIGGVNLLLKPNTSVQFMKLGMLSPEGTCKSFDASGNGYCRSEAAVAVLLTKKSMAKRIYATVLNAGNNTDGYKEQGVTFPSGEMQQRLVRSLYQEANISPEQVEYIEAHGTGTKVGDPQEVNGIVSVFCQSQRDPLLIGSTKSNMGHPEPASGLAALAKVVLSLEHGVWAPNVHFNEPNPDIPALTDGRLCVVSKPTPVRGGIVGINSFGFGGSNVHVILSPNAKHAPRHNDIPRLLQACGRTEAAVDAILQKGREHPKDDAFVSMLNDISGVPATSMPYRGFAIIGSKTDVFSGQGPQWYAMGRELLETNDVFRSTIEEIDKYLKKFGWLKKEKSSLMAELKKNEKTSRMDETEVAQPAIFALQAGLAKVWMNMGIKPDAIIGHSIGEVAAAWACGALSLEEATRVVFWRSRCQAAASGIGKMLAAGISQSEAEDEISAYSGTINLAAINGPKMVTLSGASDSIDKLAADFENRGIFCRILRVDVPFHSYLLDGIKADFLKSAGKVKSSKSIIPFYSTVTGGIYDGSKLDAAYWFKNIRQPVYFYPGVKKIIQDGYNTFVEIAPHPILGASVAATFKELSVKGAVVSTLRRQ",
    "response#3": "MPSGTDESAGPASDTGNSPRPFWRDQEEIVISGVSGRFPRSLYGLPKRHGKLKELNKFDAQFFSVTPKQANYMDPQVRILLEVAFEAMLDAGVNPVELRGSRTGVFVGCSASETGGALTQDPDIVTGYTLTGCVRSMFSNRLSFAFDLRGPSFSVDTACSSSLLAMQLAIDAIRQEQCDAAIVAGAHLTLTPTAALQFLKLGMLSPAGSCRSFDQSGDGYCRTEGIAAILIQRRSVAKRIYASVVHSKSNTDGYKEQGITFPGGDRQAVLLEEVYREAELDPSTVAYVETHGTGTKVGDPQEAYAITQTFCQNRNSPLLIGSVKSNMGHAEPASGLCSLAKIILAIHRGVIPPNLHFNEPNEYIEGLKDGSLKVVTEKTIFPSGSFIGVNSFGFGGSNTHLILKSNPKPKIPRPDLPRLVWFVFTGMGSQWPGMGKSLMKIPIFSAAIEKCQTALRPHGIDVINIITSSDPTIFDNILHCFVGIAACQIGLVDVMKAIGIEPDGIVGHSVGELGCSYMDGSFTAEQMILAAYYRGRASIETKLVKGTMAAVGLGAQKAKEICPPDIAVACHNGPDVCTLSGPAESITKFVKTLQQKGIFAREVNCANIAYHSKYISSCGPTLLKYLKQVIPNPKPRSSRWVSSSIPESAWETPLATHSSAEYHTNNLITLSSNKTNNEYATVLNVRTNTDGYKEQGITYPIGAMQNRLIRETYEDINLNPHDVSYVEAHGTGTKVGDPQEVNSITDFFCKNRKTPLLIGSVKSNMGHSEPASGVCSIAKVLIAMEAGQIPANLH"
  },
  {
    "reference": "MKKTTHFGFEEVPYEEKVRKVAGVFHSVAGKYDLMNDLMSMGVHRLWKRYTLDMSGVRRGDHVLDLAGGTGDLAEKFSRMVGPQGSVTLADINDSMLKMGRERLVNDGIVGNLQYVQANAECLPFPDNHFDAITIAFGLRNVTDKEAALRSMLRVLKPGKPLLVLEFSRPVIKPLNPIYDAYSFKVLPLLGKLFADDPDSYRYLAESIRKHPDQETLKAMMEEAGFEQCAYYNLSGGIVALHRGYKL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity, 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity to facilitate methylation, aerobic respiration.2. The desired protein should be able to perform a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine3. The designed protein should interact with enzymes and substrates within the menaquinone biosynthesis, Quinol/quinone metabolism, menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2 pathway to enhance or regulate its activity.4. For general function, the protein need meet that Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2). The designed protein sequence is ",
    "response#1": "MTRTLAELLAPWVEVRGDTTVYDVMNDAMSLGVHRLQEVSRAALAFAWPPGARLGSDGFLRAAIAAGATGVLTGLPLADVPALGVRVPDTRHALARIAGRFWGEPAERLALVGT",
    "response#2": "MEQKKSFDSIEEALEDMKKGKMILVVDDEDRENEGDFIVAAEKVTPEVINFMAKYGRGLICASMTEKRAEELDLELMVKHNTENHQTAFTVSIDHKDTTTGISAHERAFTIKKMIDTKTKPSDLRRPGHIFPLIAKNGGVLRRAGHTEAAVDLARMAGFYPAGVICEIMNEDGSMARLPQLIEFSKKHNLKIITIKDLIAYRLKTDSLIEQRVLKPGGRFVVLEMPLEIPPKKENQKYLATKKAKLGHLILKGSNIAKCKYNDKIKYLKTKKEKMGHLLHLDLEKEDHKSKEEVLEELSKLYEEFLFHKQTIFLEWEGEKGKAIHLGFNQEHPINVSLGFNEKSEYSNKTIEWYKKLLNLIKKLNSLKIKNLKFLISPKSSPLLGLQVQLDIEKISYPPSKKEHILEKLETKALF",
    "response#3": "MPTALQTIADWLEAHRPDASPELVPMAYDFLNHFLSAGRDRMLFAAAPGVCAQGEDFVPDALQRGATAVLAQRAFGLTLPEGLAVVPVADLRTWAGDAARAFHGEPSARLVAMGA"
  },
  {
    "reference": "MIKFGEAVLGNEIKAIVNVALGKGELIENTFTNALTRGNCVFANLRPNLIVKPLTLVVPRHNIESEIQDELFQGVIQYAVAKAVADLDLDEDLKVVVSVNVPEVPITNLNKRKLFQYFYASAKLAINRALNEYPSKEKVKKEKYRALHPLVGFRDVRLEYPPYLQIALDVPTMENLEFLLQTIPNSDHIILEAGTPLIKKFGLEVIEIMREYFDGFIVADLKTLDTGRVEVRLAFEATANAVAISGVAPKSTIIKAIHECQKCGLISYLDMMNVSEPQKLYDSLKLKPDVVILHRGIDEETFGIKKEWKFKENCLLAIAGGVGVENVEELLKEYQILIVGRAITKSKDPGRVIRMFINKMGYDIDTYRLYFDEDEDIGEEL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be optimized for orotidine-5'-phosphate decarboxylase activity, 3-dehydro-L-gulonate-6-phosphate decarboxylase activity activity in order to improve carbohydrate biosynthetic process.2. The protein must exhibit the following characteristics: Catalyzes the reversible formation of ribulose-5-phosphate and formaldehyde from 3-hexulose-6-phosphate.. The designed protein sequence is ",
    "response#1": "IGEALIGDGNELAHIDLIIGEKEGPVGQAFANGLSNLSVGHTPLTTVIRPNLMTKPATLIIPKVTVGDLDDAAKVFGPAQTAVGRAVADAVEEGYIPKDIVEDIVINVSVFIDPSAKNYRKIYQYNYGATKLAIRRAMEGYPSIDKVLAEKDRGTHPIV",
    "response#2": "IGEALIGDGNELAHIDLIIGEKEGPVGQAFANGLSNLSVGHTPLTTVIRPNLMTKPATLIIPKVTVGDLDDAAKVFGPAQTAVGRAVADAVEEGYIPKDIVEDIVINVSVFIDPSAKNYRKIYQYNYGATKLAIRRAMEGYPSIDKVLAEKDRGTHPIG",
    "response#3": "MLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGVRAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVAKEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLSDMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQL"
  },
  {
    "reference": "MREFVSGTQMKQLDRYTIQEIGIPSLVLMERAARSVYDTMIKERLPLKKVLVLCGSGNNGADGVALARMLHLSNISVDVEILGNPSHFTTEMKQQIEIAKKYQVAFVKNKSLSEYTTIVDAIFGVGLDREVGGGYKEKIKEINQADAKVVAVDIPSGIDSATGKVLGCAVCADWTVSFAYEKLGTVFYEGAEYAGNVRVADIGIVKMPEICFPISTYTFEEKDLKEKLFRRESGNKGTFGKVLLIAGNEMTSGAALLSASSVLHTGAGMVKVHTRKENRAILPVQLPEAMYCFYGKKEIKSEALAESLEWADVVGIGPGLGTNKQAEALLEFVLKYSKRPLVIDADGLNLLKSKLDLLEKYEYPVIMTPHLGEFSRLSGLSIKDWKQQPIELTNELAHRYNVTLVCKDARTVIADGGQNVYVNTTGNSGMATAGSGDVLTGILLGLLAQGYAPLDAAVMGVCVHGLAGDEAAKEKGKYAMVAGDLITYTGDVLKKRG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have a high specificity for (6S)-NADPHX binding, with minimal off-target effects.2. The protein design must be able to perform NADHX epimerase activity, NADPHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding in order to contribute to nicotinamide nucleotide metabolic process.3. The protein requires Mg(2+) to activate a critical catalytic site.4. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration The designed protein sequence is ",
    "response#1": "MREGDRRATGELGIPGALLMENAGYQAARAARELVPGLSGRAVAIVCGTGNNGGDGLVLARHLADLGAQVTVYLAGAAERLSRDARTMLAAWRGRGGTWVEDLPRDLEGYALVVDAVVGTGSSGALRSPAKEAAELISRVDCPVLALDLPSGVDADTGAVSGPAVRASMTVTFAALKPGLLFFPGAGLAGRVVVAGISLPPGALLLVVAGSDSYRGAALLALLGASASGLGSLRAAVPEAVADQLWSQLPHAVLSARLPSRGDGSLDLSALAPTALERLDALVLGPGIGNTADDAAIWETLQAFPGLLVLDADGLNRLAQCAALPWLRGRSGPSWLTPHPGEFQRLFPDLAGLPPLEACAAAAQQSGCSVLLKGARSVIADPSGERWQLRSASDHAARAGLGDVLAGYAGGLGALAQASGAGCSGSLLALAALAHASAGLQVSTLHGIGAATPHQIAEMLVASGGATRTGAARLAARAALRIGAGVVTVASPPEALGVNAAQLTAIMLRGCDGPEGLHAILADERLNALVVGPALGVTAVTRAMVAVAAQARRRLVLDADALTSFEGLASELHQGFRSAPTVITPHDGEFTRLFKGHPDILDPSSKVERARRAAAYLSAVAVLKGADTVIAAPDGRAAINANGTPYLGTAGSGDVLCGIIAGLMAQHLPAFEAAAAAVWIHAEAGAAFGKYQRGRLLVVAGSDSYRGAALLALLGASASGLGSLRAAVPEAVADQLWSQLPHAVLSARLPSRGDGSLDLSALAPTALERLDALVLGPGIGNTADDAAIWETLQAFPGLLVLDADGLNRLAQCAALPWLRGRSGPSWLTPHPGEFQRLFPDLAGLPPLEACAAAAQQSGCSVLLKGARSVIADPSGERWQLRSASDHAARAGLGDVLAGYAGGLGALA",
    "response#2": "MRKADASVIRSGTPGVTLMQNAGAAVADAAREMVSPGARIVVLAGPGQNGGDGIVAATRLREQGYRVSLGLLGKREAFSGDAALVAREWPSDVVAVDGANLAPAELVIDALFGGGLSREIEGAARAAIDRINMSGGPVLAVDLPSGIDGETGAVRGIAVRAARTVTFAARKPGHYLLPGRTFCGDVTVADIGVGSSDLVGPGEFVSYKFNPDESHKGENGILVGIAGSVDYYGAPIYMSKGALRMGIDLLFLFSPEYIHNYISSSTHDIILKKSGERYIEFNNELKRIIEDKATAVALGCGIGKNEVSQESSTKIIEESLLVKKPIVIDADALDSVTLFDDLKGFAVITPHRGEFKRIFSIDPDENNVVKMAKKYNLVILLKGSVDIISDGEIVKKNKSFHHPSMTRGGTGDILTGAVGGLLSRGIDTFHSATLASFIVGSAGKMAFEEKSNSYFTSEVIDMIPQVFRKYVESHKGENGILVGIAGSVDYYGAPIYMSKGALRMGIDLLFLFSPEYIHNYISSSTHDIILKKSGERYIEFNNELKRIIEDKATAVALGCGIGKNEVSQESSTKIIEESLLVKKPIVIDADALDSVTLFDDLKGFAVITPHRGEFKRIFSIDPDENNVVKMAKKYNLVILLKGSVDIISDGEIVKKNKSFHHPSMTRGGTGDILTGAVGGLLSRGIDTFHSATLASFIVGSAGKMAFEEKSNSYFTSEVIDMIS",
    "response#3": "MRKADASVIRSGTPGVTLMQNAGAAVADAAREMVSPGARIVVLAGPGQNGGDGIVAATRLREQGYRVSLGLLGKREAFSGDAALVAREWPSDVVAVDGANLAPAELVIDALFGGGLSREIEGAARAAIDRINMSGGPVLAVDLPSGIDGETGAVRGIAVRAARTVTFAARKPGHYLLPGRTFCGDVTVADIGVGSSDLPEATPVRTWFNPDESHKGENGILVGIAGSVDYYGAPIYMSKGALRMGIDLLFLFSPEYIHNYISSSTHDIILKKSGERYIEFNNELKRIIEDKATAVALGCGIGKNEVSQESSTKIIEESLLVKKPIVIDADALDSVTLFDDLKGFAVITPHRGEFKRIFSIDPDENNVVKMAKKYNLVILLKGSVDIISDGEIVKKNKSFHHPSMTRGGTGDILTGAVGGLLSRGIDTFHSATLASFIVGSAGKMAFEEKSNSYFTSEVIDMIPQVFESHKGENGILVGIAGSVDYYGAPIYMSKGALRMGIDLLFLFSPEYIHNYISSSTHDIILKKSGERYIEFNNELKRIIEDKATAVALGCGIGKNEVSQESSTKIIEESLLVKKPIVIDADALDSVTLFDDLKGFAVITPHRGEFKRIFSIDPDENNVVKMAKKYNLVILLKGSVDIISDGEIVKKNKSFHHPSMTRGGTGDILTGAVGGLLSRGIDTFHSATLASFIVGSAGKMAFEEKSNSYFTSEVIDMIS"
  },
  {
    "reference": "MILPDFSKAKVLIVGDLMLDRYWSGGTGRISPEAPVPVVNVQSSEDRPGGAANVAVNVATLGAKVTLLGMCGNDENAKLLRERLESFDIDCQFFAVEGQDTITKLRVMSRNQQLLRLDFEKSFADSDKSELESAFDGALDDVDIVILSDYAKGCLSNPQALIQAAKAKGKRVIVDPKGSDFKKYANATLITPNMEELNHVVGESTTEKSLVSNAQQVKTELNLDALLLTRSEQGMTLFEGDNAEFHLPAKAKEVYDVTGAGDTVVSTLAVALASKLPLQAACVLANLAASVVVGKLGTSTVTNTELALALGEQSVHLDGGVMTEGQLEMAMAAAKARGERIVMTNGCFDILHSGHVAYLEEAAQLGDRLIVAVNTDASVTKLKGPGRPVNNTNRRMAVLAGLSAVDWVVPFTEDTPQRLIASLLPDILVKGGDYQIDEIAGGKEVIANGGEVQVLTFEDGVSTTGIIERITRNKLT",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be able to perform ATP binding under specific conditions.2. A protein that can modulate the overall flux of metabolites through the ADP-L-glycero-beta-D-manno-heptose from D- glycero-beta-D-manno-heptose 7-phosphate: step 3/4 pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MTNGCFDILHAGHVQYLQQARQLGDYLLVAVNDDASVSRLKGDERPVNQLADRMAVLDALEAVDWVVPFSEDTPEDLINKVLPEVLVKGGDYQVNEIAGADAVIKNNGEVKIFSLLEGCSTSRIIEA",
    "response#2": "MTNGCFDILHPGHVSYLENARKLGDRLIVAVNCDQSVKRLKGESRPINSLSARMAILAGLASVDWVIAFEEDTPQRLISEILPDVLVKGGDYKPEQIVGCQEVWANGGNVKALNFEDGCSTTDVIE",
    "response#3": "MTNGCFDILHPGHVSYLENARKLGDRLIVAVNCDQSVKRLKGESRPINSLSARMAILAGLASVDWVIAFEEDTPQRLISEILPDVLVKGGDYKPEQIVGCQEVWANGGNVKALNFEDGCSTTDVIR"
  },
  {
    "reference": "MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGFINNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTAKLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEVKGIKDVVTQPQA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be optimized for outer membrane protein complex-specific folding and stability, while maintaining identical protein binding. The designed protein sequence is ",
    "response#1": "SGLVQAVNRKDGQIKWSTALKNNIVSGPTVAAGYVAVGTNASTLVLLNQSDGKEIWQNKVSAEVLAPPAISHQKVIAKTIDGKVYAIDAVNGKQLWVADHGAPSLVLKASSSPIIVDDLVLVGFSDGKLDALELQTGRLIWQRSIAYGTGASDVERLVDIDSDPIISNNVAYLATYQGYVGALSLSNGQFIWRKPASVYKNMLLSHNNLYFTDSNDVLWSLNSSTGQVNWKC",
    "response#2": "SGLVQAVNRKDGQIKWSTALKNNIVSGPTVAAGYVAVGTNASTLVLLNQSDGKEIWQNKVSAEVLAPPAISHQKVIAKTIDGKVYAIDAVNGKQLWVADHGAPSLVLKASSSPIIVDDLVLVGFSDGKLDALELQTGRLIWQRSIAYGTGASDVERLVDIDSDPIISNNVAYLATYQGYVGALSLSNGQFIWRKPASVYKNMLLSHNNLYFTDSNDVLWSLNSSTGQVNWKL",
    "response#3": "SGLVQAVNRKDGQIKWSTALKNNIVSGPTVAAGYVAVGTNASTLVLLNQSDGKEIWQNKVSAEVLAPPAISHQKVIAKTIDGKVYAIDAVNGKQLWVADHGAPSLVLKASSSPIIVDDLVLVGFSDGKLDALELQTGRLIWQRSIAYGTGASDVERLVDIDSDPIISNNVAYLATYQGYVGALSLSNGQFIWRKPASVYKNMLLSHNNLYFTDSNDVLWSLNSSTGQVNWKC"
  },
  {
    "reference": "MKPIFEKPSLYSYTLPMFKDLLKENGFKPFVADQLYSWVYVKFEFDMEKWTNIPKALKEFIKENLDTSIPKIVWNGLSKDGTRKFLVAFRDDKTVETVMIPAKNNRKTLCVSSQVGCAIGCTFCHTGTMGLTRHLEAGEIVGQFLAVTKFLKINDGEESRLSNLVYMGQGEPLHNFENVKLATEVLLEPKGLGISQRRVTLSTSGLVPQIEKLTEFPPINWAISLHAAHNEIRSELMPINKAYDLDRLLGAIKKIPLKAHRHITYEYLLIDKYNDRKEDIEALVNLLPKRVSKVNLIPFNEYPESSFKRPSVSRITWFKDQLESNGITCTVRTQKGDDILAACGQLKSEMDKKLNLWDGEEENS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. [4Fe-4S] cluster is required for the proper folding and stability of the protein.2. The protein must be able to rRNA (adenine-C2-)-methyltransferase activity. The designed protein sequence is ",
    "response#1": "SSQAGCGMNCPFCATGQAGLTRNLSTGEIVEQVAGGQRALNRGDIAGGPGRVSNIVFMGMGEPLANYKAVLGAVRRITEPSPSGLGISQRGVTVSTVGLVPAIHKLTEEGLSVRLAVSLHAPDDELRDTLVPINTRFKVREVLDAAFGYARKSGRRVSIEYALIKDVNDHAWL",
    "response#2": "EEDRGTLCISSQVGCTLTCTFCHTGTQTLVRNLTAGEIAGQVLVARDRLGEWPHLIKDAENTPASGRFITNVVLMGMGEPLYNFDNVRDAVDVISHGDGLSVSKRRITLSTSGVVPEIVRAGEEIGTMLAISLHGTTDQIRNELVPINKKYPISQLLDACRNYPGLSNARRVTFEYVMIKDVNDTAEDARRLVQLLKGIPAKVNLIPFNPWPGTSYECSDWEQIEVFARIVLKAGYASPVRTPRGRDIAA",
    "response#3": "QHPAFRSRQIYAALYRQRVEDFSGMTVLPAALRAELARTCTVGLPEVSAVFESADGTRRYLLRLADGRSVEAVFMPEESRDTLCISSQVGCPVDCKFCLTAKMGLERNLTAGEIVGQVLLLVRRHHLDPGSRQMNVVMMGQGEPLLNLPNVIAATRILADKEGVGISERRITVSTAGIVPRIAELGRESVRPKLAISLNASSEEQRRELMPITRKYPLRDLLEACRSYPLRSWERLTFEYVLLGGVNDTDQDARRLVRLVASLPCKVNLIALNPGPGIPFQTPDEARVAAFQAIVARSVPCFVRKPRGRDIFAACGQLKSL"
  },
  {
    "reference": "MSSKLVDAINEVAVSNKDKIELDGIKDDSFDGHLSITEATYDVDTFLKPSPALSKDWIRKLQKKWDREITYKGLYEYPETLARTQIRFQRHGLEGKIMGYKEVPELIEDLNSKNSSSFLRKPSSKNEFVRGSTSNIPFLADDSDVDAIAGEPSVKMALYGEDGLLQVPPGFSRGLSMTATSTTDNLNDEFDPEKWDTKKVKSSNRNFVTIHELNEHLKNVNSKHSEIDDLLPDKRSIVSLPPSTLNLHKQPDYAHVVDSSAPIENFQQLVPEMALDFPFELDNFQKEAIYHLEMGDSVFVAAHTSAGKTVVAEYAIALAQKHMTKAIYTSPIKALSNQKFRDFKHKFEDVGILTGDVQVNPEGSCLLMTTEILRSMLYRGADLIRDVEFVIFDEVHYVNDLERGVVWEEVIIMLPPHVTLILLSATVPNTKEFASWVGRTKKKNIYVISTLKRPVPLEHYLWVKQNMFKIVDQHGRFLMDGYKSANDALKKPDKPVIAKDNKNSARGRGAARGRGVQTNMMRGRGSAKSVERRDANTWVHLIGHLHKQNLLPVIVFVFSKKRCEEYVDTLTNRDLNNHQEKSEVHVVIEKAVARLKKEDRLLPQIGRMREMLSRGLAVHHGGLLPIIKEIVEILFQRGLVKVLFATETFAMGVNMPAKSVVFSGTQKHDGRNFRDLLPGEYTQCSGRAGRRGLDVTGTVIILSRSELPDTASLRHMIMGPSSKLISQFRLTYNMILNLLRVETLRIEDMIKRSFSENVNQTLVPQHEE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that localizes to cytosol and can perform hydrolase activity with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "MLIGVISDTHIPDRASEIPEAVFDAFRDVELILHAGDLTSPDILTELETLAPVECVQGNMDRHYGIETPRSRLFEIESFRVGLIHGEVYPRGDTQQLRYLGLELGADVLISGHTHQPFIRELEDMVLLNPGSPTVPRLTDPSVMVLRIA",
    "response#2": "MLIGVISDTHIPDRASEIPEAVFDAFRDVELILHAGDLTSPDILTELETLAPVECVQGNMDRHYGIETPRSRLFEIESFRVGLIHGEVYPRGDTQQLRYLGLELGADVLISGHTHQPFIRELEDMVLLNPGSPTVPRLTDPSVMVLRIR",
    "response#3": "MLIGVISDTHIPDRASEIPEAVFDAFRDVELILHAGDLTSPDILTELETLAPVECVQGNMDRHYGIETPRSRLFEIESFRVGLIHGEVYPRGDTQQLRYLGLELGADVLISGHTHQPFIRELEDMVLLNPGSPTVPRLTDPSVMVLRIG"
  },
  {
    "reference": "MDELKVFSGNAHPALARAICEYLGIPLGKAEVFEFSNENIFVRILENVRERDVFVIQPLSSPVNKNLVELLIMIDAFKRASAGRITAVIPYYAYGRTDKKDQPRVPITARLIADLLTTAGANRLLTIDLHAAQIQGFFNIPVDELTALSILSDYWKQKALENVAVVATDIGISARARDVAAKLNAPLAIIEKRRMGNIDVTETLNVIGEVDGMRALTIDDEIDTAGSLVKAVETLLEHGATEVYACCTHPVFSGPARERIAKSPVKEVVVTDTLPVSKDKMLDKITVLPTAPLLGEAIHRIHTGLSVGAMFE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have a active site active site that is highly conserved among related enzymes.2. The protein should have magnesium ion binding, ribose phosphate diphosphokinase activity, ATP binding and be active in cytoplasm.3. The presence of Ribose-phosphate pyrophosphokinase N-terminal domains is critical for the interaction of the designed protein with its intended targets.4. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.5. The D-ribose 5-phosphate, ATP binding site should be stable and able to withstand changes in environmental conditions.6. A protein that is capable of catalyzing reactions within the Metabolic intermediate biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MKVFAGNANPALAMKIADYIGIPMGNLVIQRFTDGETFVKICDNIRGKDVFVVQPLCYPPNEMIMELLIMVDAMKRASAERITAVLPYYGYARQDRKDQPRVPITAKLVANLLVAADEKVHADLHAAQIQGFFDVPVAMPILTRYFKAKEISDLVVVAVDIGISKRARDVAESLNAPLAIIEKRRLGNHDRTEILNVIGDVHGKTALTFDDEILTGGTVVNAAKALVDAGVTEVFCCATHPVFSKEALDILHHFKKMDDVMIVSPDVGGVARARELAQRIGAPLSIVDKRREKAGEVAEMTVIGDVKGKRCIIVDDICDTAGTLCKAADVLMEAGATEVHSYITHGVLSGPAVERITKSVMKKLAMPILTRYFKAKEISDLVVVAVDIGISKRARDVAESLNAPLAIIEKRRLGNHDRTEILNVIGDVHGKTALTFDDEILTGGTVVNAAKALVDAGVTEVFCCATHPVFSKELLNDSLASPVLLGDLRQKNYDDLIAVSPDVGGVVRARALAKQLNCDLAIIDKRRTKANVSEVMHLIGEVDGRNCVIMDDMIDTAGTLLKAAEVLKARGAKKVYAYCTHPIFSGPAIERIVSGDVLDEVVVTNATLFDNTDTLHELGLHIEHLAR",
    "response#2": "MKIFAGRAGRNLANLMCDHLNIPLAEGHTELFPDGELIVKVNEDVRGRDCFVVQSTCDPVNANLMELLIYIDCLRRASAKRITAVIPYFGYARQDRKDEGRTPITAKLVANLIATAERVKKDLHAAQIQGFFDLPVDHLYAAPVINEYLRNKGYSDDNIVVVSPDEGSIKRAIGHVKRLGGNLAIVDKRRASAMETTQQNLIGGPIEGKVALMFDDMISTAGSICGAAELVAAAGASEIHVAATHGVLCGPAIEASPVLLGDLRQKNYDDLIAVSPDVGGVVRARALAKQLNCDLAIIDKRRTKANVSEVMHLIGEVDGRNCVIMDDMIDTAGTLLKAAEVLKARGAKKVYAYCTHPIFSGPAIERIVSGDVLDEVVVTNTIPLSEAAKQAMPILTRYFKAKEISDLVVVAVDIGISKRARDVAESLNAPLAIIEKRRLGNHDRTEILNVIGDVHGKTALTFDDEILTGGTVVNAAKALVDAGVTEVFCCATHPVFSKEAAPLMVCYLRRKLPAENRVVVAPDPGGLKMAYSYSQLLNAGLALAGKHRKSATEVEALELVGDVAGKDCILTDDITTTAGTLCAAANLALEHGAKSVRAAVSHCLLTDMGIQRLKESRLEELITTDTVKLGEAMPILTRYFKAKEISDLVVVAVDIGISKRARDVAESLNAPLAIIEKRRLGNHDRTEILNVIGDVHGKTALTFDDEILTGGTVVNAAKALVDAGVTEVFCCATHPVFSKEASPVLLGDLRQKNYDDLIAVSPDVGGVVRARALAKQLNCDLAIIDKRRTKANVSEVMHLIGEVDGRNCVIMDDMIDTAGTLLKAAEVLKARGAKKVYAYCTHPIFSGPAIERIVSGDVLDEVVVTN",
    "response#3": "MKIFAGRAGRNLANLMCDHLNIPLAEGHTELFPDGELIVKVNEDVRGRDCFVVQSTCDPVNANLMELLIYIDCLRRASAKRITAVIPYFGYARQDRKDEGRTPITAKLVANLIATAERGVRNVLTLDLHAAQIQGFFDIPVDNLYAAPVINEYLRNKGYSDDNIVVVSPDEGSIKRAIGHVKRLGGNLAIVDKRRASAMETTQQNLIGGPIEGKVALMFDDMISTAGSICGAAELVAAAGASEIHVAATHGVLCGPAIEAAPLMVCYLRRKLPAENRVVVAPDPGGLKMAYSYSQLLNAGLALAGKHRKSATEVEALELVGDVAGKDCILTDDITTTAGTLCAAANLALEHGAKSVRAAVSHCLLTDMGIQRLKESRLEELITTDTLPIGAPRETLPRLKLLSVAPLLARAIREVFDDGSVTGLFDGQA"
  },
  {
    "reference": "MGCSNCQPEIKTSLHSKDSNDFEDDVWGDDDVYVDANADVKRAHQKQGYLDGLASAQESSLQSGFDKAFSDGARMGAAVGKILGELRALGESESFYQATKELNIAKIMDKKYFDSELKIQKNHEVIYKWQKIVAQHLEDATKA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECR",
    "response#2": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECR",
    "response#3": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECK"
  },
  {
    "reference": "MRAMGIQRNCHNWWRWGTIILGLMIICSAERNLWVTVYYGVPVWKDAETTLFCASDAKAYKTEKHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLRCSDYNGTSYVNSTSSKHNATSNGTHSYNISAEMLGEIKNCSYXMTTELRDKTKKVYSLFYRPDIVPLNGNSSSNKYTLINCNTSAIKQACPKVSFEPIPXHYCAPAGFAILKCNNKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEQIMIRSENITNNAKNILVQFTSPVKINCTRPNNNTRKSVRIGPGQAFYATGDIIGDIRQAHCNISQAEWNETLGXLTTQLRTHFGNKTINFTSASGGDLEITTHSFNCRGEFFYCNTSRLFNSTWWANGTRTGYNSTDGNITLPCRIKQIINMWQRVGQAMYAPPIPGRISCESNITGLILTRDGGSDNTTETFRPGGGDMRDNWRSELYKYKVVEVEPLGVAPTEARRRVVQREKRAVGLGAVFIGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNRSLNEIWNNMTWLQWDREVSNYTQLIYDLLEESQIQQEKNEQDLLALDKWASLWNWFDISKWLWYIRIFIMIVGGLIGLRIVFAVISIINRVRQGYSPLSFQTHTPNPGGLDRPRGIEEEGGEQDRSRSIRLVSGFLALAWDDL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains is critical for the interaction of the designed protein with its intended targets.2. The designed protein localizes to the host cell plasma membrane.3. The protein design should include a Di-leucine internalization motif motif to ensure proper function. The designed protein sequence is ",
    "response#1": "MGAASITLTVQARQLLSGIVQQQSNLLKAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGLWGCSGKLICTTAVPWNSSWSNKSVTDIWDNMTWMQWDREISNYTNTIYRLLEDSQTQQEQNEKDLLALDSWNNLWNWFNITKWLWYIKIFIMMVGGLIGLRIIFAVLSVVNRVRQGYSPLSFQTR",
    "response#2": "MTEEVKQCSFYTSTGIRGKKKKAQALFYRPDLSPLEKNNNSKNFSEYILISCNTSTITQACPKVSFEPIPIHYCTPAGYAMLKCNDNTFNGTGPCINVSTVQCTHGIRPVVSTQLLLNGSLTEREILIKSENLTNNVKTIIVHLNESVEIMCTRPNNNIRRSVRIGIGPGHTFYATGDIIGDIRQAHCNITRVKWNGTLERVRKELGKKFPNKTIKFEPHSGGDLEVTTHTFNCRGEFFYCKTTKLFNDSDLNTNNATNITLPCRIKQFINMWQQVGRAMYNPPIEGNITCKSNITGLLLTRDGGKSGNDTGKEIFRPGGGNMKDNWRNELYKYKVVEVKPLGIAPTEAKRRVVEREKR",
    "response#3": "NATDNQAQEEIKNCSFDMTTELKDKKKRVYSLFYRLDIVELNASSNEYRLINCNTSAITQACPKVTFEPIPIHYCAPAGFAILKCNDKQFNGTGLCKNVSSVQCTHGIKPVVSTQLLLNGSLAEGNITIRSKNITDNAKTIIVQLAEPVTITCIRPFNNTRKSIHIGPGRAFYATGDIIGDIRQAHCNVNKTNWNKTLGEVAQKLRQYWNKTIIFTSSSGGDIELTTHTFNCRGEFFYCNTSNLFNWNGTWNGNTTGNDTITLQCRIKQIINMWQRVGQAMYAPPIPGEIKCESNITGLLLTRDGGNSTDNETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPNKARRRVVGREKR"
  },
  {
    "reference": "MDNFQYSVQLSDREWAEFSATADECGLLQADLASGDEPLSSDIDQGDSSGSSPPGPPPLFTGQLVSQGRGQQSRELEDVAAQQLVSRSQCEPVLALEASHQVAGTSTQSEAPLFPSLDSVCPGQALSFPGPATCRDKMQRLLQGPAPSSPSKAPHSPESPGHSDNPQSSPDSLEASPRNPGRKKRRAVGAKGTKHSGSLDSAATQMSSPQLTRPKEALMSGTLMAKAQQDKPQPDSTSPEQMAREESGLDLSTPILITEQDQIRKTSRAALHVVSKPVQEVHPDVPMASPNVSTRASKPQPDVALPKPASKPQSDIASSTHPFTPDVSLSTLAFKCQPNTNQSTPASEPDMALSTPASEPDTALSTPASEPDTALSTPASEPDTALSTPASRSQLVKAEFASACTPGLHVDLSAAGSEIKPEVSSSMPAAVDVLRTDLPRSVSKTESKESVSIPVKPSLSPISQAEAAMVDTGVSVPPGGSIEKPAGQFSAGSSGESCLGPVQAPKKKKVRFSMAIPSHEDSGSGEPTGSPFLTPEQPPVPRTAAGSRGGSAAWDAVAVAPRLPQPRILKHLPPPVSSVSAEAESGNCFAVTLPEAYEFFFCDTIEEEDEDVEDEEAASQALDEVQWPDTCEFFFRDSRAQRSRCQRGHSPVSPPRADTVAPVPPEGLVPISIPEVYEHFFTEEGFGHRQPAATPMQTSELSREGGLEASTKPVSPTAEHLSLTVRKAGELRSPLTSFTFSQNDMCLVFVAFATWAVRTSDLQAPDAW",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a specific function in response to muscle activity, regulation of DNA-templated transcription and should be localized to nucleoplasm, nucleus in order to carry out this function efficiently.2. For general function, the protein need meet that Required for the efficient induction of MT-CO2, MT-CO3, COX4I1, TFB1M, TFB2M, POLRMT and SIRT3 by PPARGC1A; Regulates the expression of selective PPARGC1A/B and ESRRA/B/G target genes with roles in glucose and lipid metabolism, energy transfer, contractile function, muscle mitochondrial biogenesis and oxidative capacity3. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "LSAEQSNVLELVTQHKKSVFFTGSAGTGKSVLMRAIIAELRKVYKREPDRVAVTASTGLAACNIGGVTLHSFGGIGLGKEPVPDLVKKIMRNQKAKNRWLRTKVLVVDEVSMVDGELFDKLEGIARFIRKNGRPFGGIQLVVTGDFFQLPPVPDYGKQVKFAFDADTWSTSIHHTIGLTEVFRQRDPVFANMLNEMRLGKISDAA",
    "response#2": "TMVFISNESDVVENLDEIYHTGTFAQIHEMQDLGDKLRMIVMGHRRVHISRQLEVEPEELEAENKHKPRRKSKRGKKEAEDELSARHPTELAMEPAPELPAEVLMVEVENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLPRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNIPKRLYKALSLLKGL",
    "response#3": "LSAEQSNVLELVTQHKKSVFFTGSAGTGKSVLMRAIIAELRKVYKREPDRVAVTASTGLAACNIGGVTLHSFGGIGLGKEPVPDLVKKIMRNQKAKNRWLRTKVLVVDEVSMVDGELFDKLEGIARFIRKNGRPFGGIQLVVTGDFFQLPPVPDYGKQVKFAFDADTWSTSIHHTIGLTEVFRQRDPVFANMLNEMRLGKISDAGRELLHKITDCYGYKSHREIVTITASHRANHLITQFFNGQERALHERDEQAGSDPSVFLHGTIDADGRTMSYEPRAVLWDAKGGSGALGRFQYWSQDDYADEEEPPRAAPGIEVVQTAARVRRSAYQRALG"
  },
  {
    "reference": "MTDEKVINKKLVGDNKLFRHQAKFVAGAMDIKQLPNFALPQIAFVGKSNVGKSSLINTICNNKKLAKVSNTPGRTRQINFFNLVDKLIIVDLPGYGFAQVPNQVKDQWEILINHYLRKSDNLKLVNLLIDSRRGIKENDKKVAELLLANKRDFQIIFTKSDKVTDRKNLNLEAQNFLATLNYSCNLIYVSSRSKEGARELKTSLAKCIKLEK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Necessary for normal cell division and for the maintenance of normal septation.2. The protein design should aim to mimic or enhance the function of natural proteins involved in division septum assembly, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "VITKPLRSGQRVYARHTDLIVIGMVSQGAEVIADGNVHVYGPLRGKAMAGARGDTSARIFTTQLDAELLAVAGVYRVVEDKLDRALHNQPALVRLDGDTLRIY",
    "response#2": "VITKPLRSGQRVYARHTDLVVIGMVSQGAEVIADGNIHVYGPLRGKAMAGARGDTSARIFTTHLDAELLAVAGVYRVVEDRLDRNLHNQPALVRLNGDTLHIL",
    "response#3": "VITKPLRSGQRVYARHTDLVVIGMVSQGAEVIADGNIHVYGPLRGKAMAGARGDTSARIFTTHLDAELLAVAGVYRVVEDRLDRNLHNQPALVRLNGDTLHIL"
  },
  {
    "reference": "MKLGIIPYQEGTDIVYKNALQGQQEGKRPNLPQMEATHQIKSSVQGTSYEFVRTEDIPLNRRHFVYRPCSANPFFTILGYGCTEYPFDHSGMSVMDRSEGLSISRDGNDLVSVPDQYGWRTARSDVCIKEGMTYWEVEVIRGGNKKFADGVNNKENADDSVDEVQSGIYEKMHKQVNDTPHLRFGVCRREASLEAPVGFDVYGYGIRDISLESIHEGKLNCVLENGSPLKEGDKIGFLLSLPSIHTQIKQAKEFTKRRIFALNSHMDTMNEPWREDAENGPSRKKLKQETTNKEFQRALLEDIEYNDVVRDQIAIRYKNQLFFEATDYVKTTKPEYYSSDKRERQDYYQLEDSYLAIFQNGKYLGKAFENLKPLLPPFSELQYNEKFYLGYWQHGEARDESNDKNTTSAKKKKQQQKKKKGLILRNKYVNNNKLGYYPTISCFNGGTARIISEEDKLEYLDQIRSAYCVDGNSKVNTLDTLYKEQIAEDIVWDIIDELEQIALQQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that The COMPASS (Set1C) complex specifically mono-, di- and trimethylates histone H3 to form H3K4me1/2/3, which subsequently plays a role in telomere length maintenance and transcription elongation regulation.2. The designed protein should be involved in histone H3-K4 trimethylation, telomere maintenance by localizing to Set1C/COMPASS complex and exhibiting transcription cis-regulatory region binding. The designed protein sequence is ",
    "response#1": "EGMTYWEVEVIRGGNKKFADGVNNKENADDSVDEVQSGIYEKMHKQVNDTPHLRFGVCRREASLEAPVGFDVYGYGIRDISLESIHEGKLNCVLENGSPLKEGDKIGFLLSLPSIHTQIKQAKEFTKRRIFALNSHMDTMNEPWREDAENGPSRKKLKQETTNKEFQRALLEDIEYNDVVRDQIAIRYKNQLFFEATDYVKTTKPEYYSSDKRERQDYYQLEDSYLAIFQNGKYLGKAFENLKPLLPPFSELQYNEKFYLGYWN",
    "response#2": "KPVKFARSAIHNWGLYAIEPIAANEMIIEYVGEVVRQEIADLREARYMRSGIGSSYLFRVDESTVVDATKRGGIARFINHCCTPSCTAKIIKVEGQKRIVIYASRDIAANEELTYDYC",
    "response#3": "KPVKFARSAIHNWGLYAIEPIAANEMIIEYVGEVVRQEIADLREARYMRSGIGSSYLFRVDESTVVDATKRGGIARFINHCCTPSCTAKIIKVEGQKRIVIYASRDIAANEELTYDYC"
  },
  {
    "reference": "MSGPADETAGDLPVKDTGLNLFGMGGLQETSARTMKSRQAVSRVSREELEDRFLRLHDENILLKQHARKQEDKIKRMATKLIRLVNDKKRYERVGGGPKRLGRDVEMEEMIEQLQDKVHELERQNEVLKNRLISAKQQLQIQGYRQTPYNHIQSRINTGRRKANENAGLQECPRKGIRFQDIDVAETLQPMLTKYDNSLLEEARGEIRNLENVIQSQRGQIEELEHLAEILKTQLRRKENEIELSLLQLREQQATDQRSNIRENVEMIKLHKQLVEKSNALSVMEGKFIQLQEVLTLRISHDALVANGDELNMQLKEQRLKCCSLEKQLHSMTFSARRVEELQDRINDLEKERELLKENYDKLYDSAFSAAHEEQWKLKEQQLKVQIAQLETALKSDLTDKNEILDRLKTERDQNEKLIQENRELQLQYLEQKQQLDELKNRMKFYNQESDINVDELSEALLLIKAQKEQKSGDLSFLEKVDSKINKDLERSMRELQATHAETVQELEKTRNMLIMQHKINKDYQMEVEAVTHKMENLQQDYELKVEQYVHLLDIRAARIQKLEAQLKDIAYGTKQYKFKPEIMPEDSVDEFDETIHLERGENLFEIHINKVTFSSEVLQASGDKEPVTFCTYAFYDFELQTTPIVRGLHPVYNFTSQYLVHVNDLFLQYIQKNTITLEVHQAYSTDYETIATCQLRFHEILEKSGRIFCTANLVGKYRSSVHTCSREYCQQVPKTAQLSSTDSTDGNLNELHITIRCCSHLQSRAGH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must contain a signal peptide for proper functionality.2. The designed protein should have thromboxane A2 receptor binding within centrosome, cytosol, ciliary basal body, plasma membrane, nucleoplasm to regulate cochlea development, non-motile cilium assembly, liver development, embryonic forelimb morphogenesis, lateral ventricle development.3. Target a Acidic residues compositional bias in the protein's composition for improved properties.4. The designed protein should contain one or more C2 domains that are essential for its function. The designed protein sequence is ",
    "response#1": "SNLLALSVLAGARPGAGPRSSFLALLCGLVLTDFLGLLVTGAVVASQHAALLDWRATDPGCRLCHFMGAAMVFFGLCPLLLGAAMAAERFVGITRPFSRPAATSRRAWATVGLVWVGAGTLGLLPLLGLGRYSVQYPGSWCFLTLGAERGDVAFGLMFALLGSVSVGLSLLLNTVSVATLCRVYHAREATQRPRDCEVEMMVQLVGIMVVATVCWMPLLVFILQTLLQTLPVMSPSGQLLRTTERQLLIYLRVATWNQILDPWVYYFTGDGFRDNLRKVLFRRGSS",
    "response#2": "VHLERGENLFEIHISKVIFSTEAVRAFGDQEPATFCTYAFYDFELQTTPVICGLNPSYNFTSQYLVRVDDFFLQYIQKSTITLEVHRAFGTDYETAAACQLRFHEILENNGRIYSTAILVGTGGNIPNYGTVDYWVRLRVQ",
    "response#3": "CQPPPVKNMGPAKVQIWRSQPLRLGVTFEAAILCNISQGLSYTWSFVSAEMTTVTLPTAVNTRRQTIMLPSYTLECGNYTAIAKVQIKGSMVYSNYCVGVEVRARAPVSVISEGTHIFISTATSTFIILRGAQSYDPDNPGAALRYHWTCTAASSPRWPCFDNSTSYQVDTQAPAISFPAKWLSECCDQFLVTLTVSSRGQNSSQALMFLSTRPDLAFRFVHISWVNFRDISVNWNEEVSLRAVCEDCGDVPDLTYSWDLFLVNATEKSAVEVPFCSTVGLLGALALGTSLKSSKSDLPSNLRAPLTPHSPC"
  },
  {
    "reference": "MSNSILSKLNVGERSPVNRTHSSRTEAEKSSLFRFVRRKINSPWLACVPLTALIVAIWNAAAIAQDTEIVNITVETVNENVATLQGTLNAIWILIAAILVIFMNAGFGMLETGLCRQKNAVNILTKNLIVFALATIAYWAIGFSLMFGSSGNPFVGFGGFFLSGDHTNYGLSPFPEGLPVAVFFLFQVAFSATAATIVSGAVAERIKFNEFLIFSVLLVGIAYPITGHWVWDAGGWLYTMGFMDFAGSTVVHSVGGWAALAGAFLLGPRLGKFVDGRPGAIPGHNMGFAMLGCLILWIGWFGFNPGSQLAADQACAYIAVTTNLAASAGGLTATFTSWLKDGKPDLTMVINGVLAGLVGITAGCAGVSYWGSVIIGGIAGILVVYSVAFFDKIKIDDPVGAISVHLVNGVWGTLAVGFFNMEKGLFYGGGINQLIIQIVGILAIGAFTAIFSFVVWAILKQTMGIRVSGEEEMIGLDIGEHGMEAYTGFVKETDSFGSAVSGATVPE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "TIGLCFLVALMEGLDLQAAGIAAGGIAQAFALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWDFPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGFAGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQSAPPLRALFAPETATATLLLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDKLRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSSQIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMSRRT",
    "response#2": "TIGLCFLVALMEGLDLQAAGIAAGGIAQAFALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWDFPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGFAGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQSAPPLRALFAPETATATLLLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDKLRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSSQIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMSRRT",
    "response#3": "TIGLCFLVALMEGLDLQAAGIAAGGIAQAFALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWDFPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGFAGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQSAPPLRALFAPETATATLLLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDKLRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSSQIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMSRRR"
  },
  {
    "reference": "MVEKGKMVKISYDGYVDGKLFDTTNEELAKKEGIYNPAMIYGPVAIFAGEGQVLPGLDEAILEMDVGEEREVVLPPEKAFGKRDPSKIKLIPLSEFTKRGIKPIKGLTITIDGIPGKIVSINSGRVLVDFNHELAGKEVKYRIKIEEVVDDKKNIVKEIVKMYVPRLSDVKVTIRNGTVKIELPEFAPFIPNIQTAKMAIANEILKRLEDAEKVSFVETFERKKETKEENK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be designed to catalyze the following reaction [protein]-peptidylproline (omega=180) = [protein]-   peptidylproline (omega=0). The designed protein sequence is ",
    "response#1": "VLLTTSAGNIELELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVADKISQVPTHDVGPYQNVPSKPVVILSAKV",
    "response#2": "VLLTTSAGNIELELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVADKISQVPTHDVGPYQNVPSKPVVILSAKR",
    "response#3": "VLLTTSAGNIELELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVADKISQVPTHDVGPYQNVPSKPVVILSAKY"
  },
  {
    "reference": "MSRIGKRPIEIPSGVKVSYVTPILKVEGPKGSLLREIMSDIALEIEEKSVSVNRADDAIKSRSAHGLTRTLINNMVVGVTKGFEKILEINGVGYRAEAKGDVLSLSLGYSHPINFPLPKGITVEVDKMTKVFVRGIDKELVGQTAAKIRSFRGPEPYKGKGIKYADERILRKAGKTGKK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design must be focused on enhancing the efficiency of translation. The designed protein sequence is ",
    "response#1": "YDCYANLALLKLYQFNPHLSRDETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAKGTVVREC",
    "response#2": "YDCYANLALLKLYQFNPHLSRDETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAKGTVVREV",
    "response#3": "YDCYANLALLKLYQFNPHLSRDETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAKGTVVREI"
  },
  {
    "reference": "MGDMRLVVAGAAGRMGRVLVQIVQQTPGAMVSGALARADSPAIGQDAGVLAGCGEIGVKISTDPLRAVADCDGVLDFTSPASTMVLATLAAQARVVHVIGTTGLSPEHLARLDAAARHAVIVQSGNMSLGVNLLAALVEKAARTLGPEFDIEIAEMHHRAKVDAPSGTALLLGEAAAKGRNIDLPSHSLRARDGHTGARPEGAIGFASLRGGGVVGEHKVYFAGAGERLELAHVAEDRSIFARGAVKAALWGRGRKSGLYSMADVLGLGDKI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be designed to have high stability and activity towards (S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-   4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH.2. The protein should be able to modulate lysine biosynthetic process via diaminopimelate in a way that leads to a desirable outcome.3. Ideal protein should have a binding affinity for key enzymes in the Amino-acid biosynthesis, (S)-tetrahydrodipicolinate from L-aspartate: step 4/4 pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MRVGVLGARGKVGATMVAGVEAADDLTFTTGVDAGDSLSALVDSDTEVVIDFTHPSVVMDNLKFLIDNGIHAVVGTTGFTDERLDQVREWLAATPGASVLIAPNFSVGAVLMMQFSKWAAKYFPDVEIIEKHHDQKLDAPSGTAVKTAQLIEEVRDSKVQGHPEEKETIPGARGANEDGMRIHSVRLPGLVAHQEVIFGGTGETLTIKHDSHHRQSFMSGVKLAVDQVQRIHTLVYGLENLL",
    "response#2": "MRVAVSGAAGRMGRLVVKNAVAEGLKVVQAFDINEVGKDAGELAGVGKIGVPIEDDISKLDADVLIDFTTAEAAMKNAEVAAEKGVRVVMGTTGFTDEDRKRLAELAEKVPMIVSPNMSVGAVMLMEAAKLVARQMPSCEIIELHHDRKLDAPSGTALRTAELIDAEREGQPQPPGPAGDPARGAVHRGIPIHSVRLPGLVAHQEVIFGGLGQTLTIRHDSLSRQSFMPGVLLAVHKVSGLDGMVVGLEL",
    "response#3": "VKVVVSGALGRMGTNIGRIVDEAPDMELVGGIDVREGTLFKTEVVPAAKIDAFLNEKKPDVLIDFTVAGAAVENIKAAARNGVALIVGTTGFSPEQRETIAAAVEGNVPAVISSNFAIGAVLMMQFSKWAAKYFPDVEIIEKHHDQKLDAPSGTAVKTAQLIEEVRDSKVQGHPEEKETIPGARGANEDGMRIHSVRLPGLVAHQEVIFGGTGETLTIKHDSHHRQSFMSGVKLAVDQVQRIHTLVYGLENLL"
  },
  {
    "reference": "MAKKKAFISLLYLASIVFLPWWISLSFTFNKSLESWVNNWWNTRPSEILLNDIQEKSILKKFIELEELSLFDEMLKEYSERRLQKLHVGVYNETIQWIKMHNEGRIHTILHFSTNIISFVILSVFSILSNEELIFLNSCLQEFLYNLSDTIKAFSILLLTDLCIGFHSPHGWELMIGSIYKDFGFAHNDQIISGVVSTFPVILDTIFKYWIFRYLNRVSPSLVVIYHSMND",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "MPAPS",
    "response#2": "GQAK",
    "response#3": "GFAT"
  },
  {
    "reference": "MTGKRILLIVGGGIAAYKACELIRLIRKAGMSVRCVLTDGGAQFVTPMTLAALSEAQVHTSLWDLKDEAEMGHIQLSREADLVVVAPATADLLARMAAGIADDLATTLLLATDKPVLAAPAMNVRMWQHAATRRNVERLRDDGVRVLEPDEGPMACGEFGPGRLPEPEAILAAIQESLSKPFVSSEVETRFSTSLEANGELRGRHVIVTAGPTHEPIDPVRYIANRSSGKQGFAIAAALARLGARVTLVSGPVALPTPAGVDRVDVETARDMAAAVDAALPADAAVLVAAVADWRVKPSGSKIKKQGTVPSLALAENPDILAGLARGPRRPRLLVGFAAETDDVIANATAKRARKGADWIVANDVSGDVMGGADNRVHLVSEAGVEDWEEMPKEQVAARLADRIADAIA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein requires Mg(2+) to activate a critical catalytic site.2. The Proton donor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. We require a protein that can specifically bind to the metabolites produced in the CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis, Cofactor biosynthesis pathway.4. The designed protein must be optimized for metal ion binding, FMN binding, phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity. The designed protein sequence is ",
    "response#1": "LSGRKVLITAGPTFEPIDPVRGITNLSSGKMGYAIARAAREAGAEVTLISGPTGLAAPHGVNRIKVVTAQQMHDAVMARVAGHQVFIGVAAVADWRVAEVSAQKIKKKDGHDVPQLKFEQNPDILAAVAALPSPPYCVGFAAESEELLRHGEEKRRRKRTPLLVGNIGPQTFGLDDNELILFDDQGHCPLPRADKLVLARRLIAEISRRIAER",
    "response#2": "HIVLGLSGGIACYKAADLVRELVKAGATVQVVMTEAAEAFITPVTLQALSGRPVWTSQWDARAPNNMAHINVSREADAILIAPASADFMAKLAQGRADELLSLTCLARPFDTVPLLVAPAMNREMWAHPATQRNVAQLRQDGTTVLGPAQGDQACGETGDGRMLEPAELRDHIVLGLSGGIACYKAADLVRELVKAGATVQVVMTEAAEAFITPVTLQALSGRPVWTSQWDARAPNNMAHINVSREADAILIAPASADFMAKLAQGRADELLSLTCLARPFDTVPLLVAPAMNREMWAHPATQRNVAQLRQDGTTVLGPAQGDQACGETGDGRMLEPAELRDVAAALL",
    "response#3": "KRILLCVSGGIAVYKASALTSKLVQAGAEVKVIMTDSAREFVTPLTFQALSRNPVYTDTFDEKDPAVIAHIDLADWPDLILVAPATANMIGKLANGIADDMISTTLLAATAPVWIAPAMNVHMYDHPAVKKNMQTLASFGYQFVEPGEGYLACGYVGKGRLEEPETIVSLVEQLAAR"
  },
  {
    "reference": "MADKKEGGVHRFYKIEDGKTVKLRHICSRCGKGFFMAQHKDRRSCGKCGLTEFNQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should be able to enhance the efficiency of translation.2. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects.3. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "MMAWMNAESVAKTLETGRVTYWSRSRQSFWIKGESSGHVQELVELRVDCDRDALLAMVRQTGPACHTNRRSCFS",
    "response#2": "MMAWMNAESVAKTLETGRVTYWSRSRQSFWIKGESSGHVQELVELRVDCDRDALLAMVRQTGPACHTNRRSCFG",
    "response#3": "MMAWMNAESVAKTLETGRVTYWSRSRQSFWIKGESSGHVQELVELRVDCDRDALLAMVRQTGPACHTNRRSCFH"
  },
  {
    "reference": "MTAVELRDALGAATGLSLPSTIAFDHPTVAALAQYLGEVLGAESGSVEAAPARVGADEPIAIVAMACRFPGGVRSADDLWALVAEGRDAIGPFPTDRGWDLDGLYHPDPDHPGTTYAREGGFVDGVDRFDADLFGINPREALAMDPQQRLLLETSWEVFERAGMDPQRLAGAPAGVFVGAATQGYGTGLAEFPEGVEGYLLTGNASSVVSGRLAYTYGLRGPALTVDTACSSALVAVHLAGQALNRGECALALAGGVMVMPTPLAFVEFSRQRGLAPDGRCKPFAEAADGTGWSEGAGMVLLERLSDARRNGHPVLAVIRASAVNQDGASNGLTAPSGLAQQRVIRQALAGAALSAADVDAVEAHGTGTALGDPIEAGALLATYGRDRAEDRPLWLGSVKSNIGHTQAAAGIAGLIKMVQAMRHGTLPPTLHVDRPTSHVDWSSGAVRLLTEAVPWPAGERPRRAGVSAFGASGTNAHVILESVEPAEPAEPAPARDRGPVLPFVLSAKTRDALRDQAERLRKHLEADPALALDDAAYSLVETRAQLPSRAVFAARDRGRVLAALGDLARDEPDPGVVTGTATGDGGRVVFVFPGQGAQWPGMARELTEQSPVFAGRLAECAAALAPHLDWSIMDVLRGVPEAPSLTRTDVVQPVLFAVMLSLAELWQAHGVRPDAVVGHSQGEIAAACLAGAIPLPVAARMVALRGKALTGLSGAGAMGSVLMPYDALAERLPGWGGRVEVAAVNGPTSVIVSGDADAVADLLDRLA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein with enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity, phosphopantetheine binding functions is required to enable fatty acid biosynthetic process.2. The designed protein have Carrier domains with specific sequence motifs that are crucial for its activity.3. We need a protein that can perform dodecanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxotetradecanoyl-   [ACP] + CO2 + holo-[ACP] with high efficiency.4. The protein must exhibit the following characteristics: Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH; This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.. The designed protein sequence is ",
    "response#1": "MYPNRISYWFDSKSPSYYVDCGCVGSMAVVHLAFNSIVAGECEAALVGGCSLCLSSTVTLNLINAGLLCNDGKTKCFDKRADGYVRSETIGFLFLQKAKDAKRIYAEIYHTKLGYCSSTLNNGQIVRHSNSKDIIKFLKKFYSEIDVSPQEVEYVEANGIANAEDDKNELEAIGEIFGNKERAVKIGCVKSNMGNSESASGLCALTKVCLAYRNGELSGNLHYENPHDDVDAVRDGRIEVVHENTPFKRRFAAVNSFSYTGPMAHVLLKYPNRISYWFDSKSPSYYVDCGCVGSMAVVHLAFNSIVAGECEAALVGGCSLCLSSTVTLNLINAGLLCNDGKTKCFDKRADGYVRSETIGFLFLQKAKDAKRIYAEIYHTKLGYCSSTLNNGQIVRHSNSKDIIKFLKKFYSEIDVSPQEVEYVEANGIANAEDDKNELEAIGEIFGNKERAVKIGCVKSNMGNSESASGLCALTKVCLAYRNGELSGNLHYENPHDDVDAVRDGRIEVVHENTPFKRRFAAVNSFSYTGPMAHVLLKGHYPHDDVDAVRDGRIEVVHENTPFKRRFAAVNSFSYTGPMAHVLLKGHYKEKNVEKYITNIPYLVNVSSRTEDNVGNMLDILAAMPIDAEYIGLLHNIHKIVTSGHTVRGYIVLITGVSGYFPDSDSVIHLQENLFNKVDLVSNDTRRWKLVHPEIPHRTGKINNLNKFDASFFGVHFKEAHTMDPMCRMMLEKAYEAVFDAGMNPKELREKKVGVFIGACFSESEKTWFYEKMVINNHGISGCSRAMLANRISYWLGVTGPSYTIDTACSSSFYALVHAYRAIRDGHCDAAIVGGCNLCLHPYVSLQFARQGVLSPDGVCKSFDNSANGYARSEAIVVVFLQKAKDSRRVYAQMIHGKTNCDGYKEQGISYPASIIQKLLLEEFYQECAIAPSVLEYLEAHGTGTKVGDPQELQAIDEIFCTGRSHPLLIGSVKSNLGHSEPASGLCSIAKMCIAYNTGFIPPNIHFETPREGVAALAEKRVSVVTEKTPWGRGMSGVNGFGFGGANAHALLKNVA",
    "response#2": "MDEIVIAGMSGRLPESNNLEEFWENLINGVDMVKEDNRRWKPGLYGLPRRNGKLKDISHFDATFFGVHPKQANTMDPQLRLMMEIAYEAIVDGGLNPASMRGSKTGVYIGVSGSEAGEAFSRDPEELLGYSMTGCQHAMLANRLSYCFDFTGPSTSIDTACSSSLLALENAFHAIRHGQCDAALVGGVNLLLKPNTSVQFMKLGMLSPEGTCKSFDSSGNGYCRSEAAVAVLLTKRSFAKRVYATVVNAGSNTDGFKEQGITFPSGEMQQRLVHSLYQEVNIPPEQVEYVEAHGTGTKVGDPQEVNGIVSVFCQSKRQPLLIGSTKSNMGHPEPASGLAALAKVLLSLERGVWAPNLHFNSPNPDIPALTDGRIQVVDRPIPIRGGIVGINSFGFGGSNVHVILRPSSKPNPPKTTLPVFSGMGSQWLTMATSMMALEPFARSIHQSAAVLKPLGIDLIELLTKNDPELWKSTVNPFVAITSMQVALYDVIKLLNIKIEGIIGHSFGEVACAYADGCLTLEQAILTSYWRGKIVEQSKLPRGVLAAIGLSWEETKKRCPPGIVPACHNGSDSVTISGLYEETKKFVEKLQAENVFARLVAGGEYPYHSPHMNAVAPDLIAALNKVIPEPKKRSSKWISTSFDKSNWFIEDACYASAKYFTNNLVSPVLFNEGMQEISKDCIAIEVAPHSLFDSIFKRCYQQHHYIGLMKRNYPNRISYWFDSKSPSYYVDCGCVGSMAVVHLAFNSIVAGECEAALVGGCSLCLSSTVTLNLINAGLLCNDGKTKCFDKRADGYVRSETIGFLFLQKAKDAKRIYAEIYHTKLGYCSSTLNNGQIVRHSNSKDIIKFLKKFYSEIDVSPQEVEYVEANGIANAEDDKNELEAIGEIFGNKERAVKIGCVKSNMGNSESASGLCALTKVCLAYRNGELSGNLHYENPHDDVDAVRDGRIEVVHENTPFKRRFAAVNSFSYTGPMAHVLLKGHY",
    "response#3": "TDDVVISGISCRLPESDNIEEFRQHLINKDDMVTDDERRWPKGLYGLPPRNGKLKDITKFDAAFFGVHPKQANSMDPQLRLLLEVTYESIIDAGVNPDTIRGSKTGVFIGCSASESHDAWSCDPEKTVGYSMTGCTRSMFANRLSFFFDFKGPSFAIDTACSSSLLAMDQALHSIRQGHCDSAIVGGCNLCLKPTTSLQFQKLGMLSKEGTCKSFDASGDGYCRSEGVVAIYLQKQNVAKRIYSTIVNSKTNSDGSKEQGITFPSGSIQKQLLNEVYSEACIDPTSVEYVEAHGTGTKAGDPQELNAICDVFCKDRSTPLLIGSIKSNMGHPEPASGLAAVTKLIIGMEDKQMPANLHYETPNTDIPGLTDGRLEVVTERTPLNGGLVAVNSFGFGGSNVHAVLKSNPKPAPLPNVPNNKIEALKNKTMKVVTEPTKLDGPIIATTTLGMPSSIGHVLLKQNPKTKEYSQLGPSQRLILLSSRTEKGISEAIDRVTSLPRDDEYLGLIQNVFKDNIVGHFHRGYAPNNKIEALKNKTMKVVTEPTKLDGPIIATTTLGMPSSIGHVLLKQNPKTKEYSQLGPSQRLILLSSRTEKGISEAIDRVTSLPRDDEYLGLIQNVFKDNIVGHFHRGYALD"
  },
  {
    "reference": "MKKKTGQLYEGAYVFSVTLSEDARRKALEKVTSGITNYGGEVLKIHDQGRKKLAYTIRGAREGYYYFIYFTVAPEAISELWREYHLNEDLLRFMTLKASAVKEVLEFATLPE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Binds together with S18 to 16S ribosomal RNA.2. The designed protein should be involved in translation by localizing to ribosome and exhibiting structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "MDIGLIINTHGLKGELKVNPQTDNIDRFKKLKRVYIAGEEKQVEGCKFLNDKVVLKIQGIDGIEIANKYRNKYIEVKREK",
    "response#2": "MPIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTL",
    "response#3": "MAPGKRRLAYPIRMSTKAGRKILARRRAKGRHSLTV"
  },
  {
    "reference": "MKAKETQRNWPNLWKWGTLILGLVIICSASDNLWVTVYYGVPVWKDADTTLFCASDAKAHETEVHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTNANFKKTNVSNIIGNITDEVRNCSFNMTTELRDKRQKVHALFYKLDIVKIEDNSSEYRLINCNTSVIRQACPKISFDPIPIHYCTPAGYAILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEKIIIRSENLTNNAKTIIVHLNKSVEINCTRPSNNTRTSINIGPGQVFYRTGDIIGDIRKAYCEINGTKWNEVLRQVTEKLKEHFINRTIIFQPPSGGDLEITMHHFNCRGEFFYCNTTQLFNNTCIGNKTIEGCNGTIILPCKIKQIINMWQGAGQAMYAPPISGRINCVSNITGILLTRDGGLNDTNETFRPGGGNIKDNWRSELYKYKVVQIEPLGVAPTRAKRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKIICTTAVPWNNTWSNRSFEEIWNNMTWIEWEREISNYTNQIYEILTESQDQQDRNEKDLLELDKWASLWNWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTPIHHQREPDRPERIEEGGGEQDRDRSVRLVSGFLAFVWDDLRSLCLFSYHRLRDFILIAAR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should include a YXXL motif; contains endocytosis signal motif to ensure proper function.2. The protein must exhibit the following characteristics: The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain.3. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.4. The presence of Retroviral envelope protein GP41-like domains is critical for the interaction of the designed protein with its intended targets.5. The protein design should emphasize Basic and acidic residues compositional bias.6. The designed protein should have structural molecule activity within host cell plasma membrane, viral envelope to regulate mitigation of host antiviral defense response, positive regulation of plasma membrane raft polarization. The designed protein sequence is ",
    "response#1": "MRVMGIRKNYQHWWRGGILLLGMLMICSAAEQLWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWVTHACVPTDPNPQEIVLGNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDANVTYTDEVKNCSFNMTTELRDKKQRVHALFYRLDIVQIKNGXKNSEYRLINCNTSVIKQACPKISFDPIPIHYCTPAGYAILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKTIIVHLNKSVEINCTRPSNNTRTSINIGPGQVFYRPGDIIGDIRKAYCEINGTKWNEVLEQVTEKLKEHFHNKTIKFRPPSGGDLEITTHHFNCRGEFFYCNTTKLFNNSCIGNRTAKGCNGTITLPCKIKQIINMWQRAGQAMYAPPISGRINCVSNITGILLTRDGGGANNETKEETFRPEGGNIKDNWRSELYKYKVVQIEPLGIAPTKAKRRVVEREKRAIGMGAVFLGFLGSAGSTMGAASVTLTVQARLLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEKIWDNMTWREWEKEIDNYTSLIYSLLEDSQNQQEKNEQELLELDKWASLWNWFSITNWLWYIKIFIIIVGGLIGLRIVFTVFSLVNRVRQGYSPLLFQTHLPAPRGPDRPEGIEGEGGERDRDTSRPLVHGFLALIWVDLRSLCLFS",
    "response#2": "MRVKGIMKNYQHLWRWGTLLLGMLMICSATEDLWVTVYYGVPVWRDADTTLFCASDAKAQETEVHNVWATHACVPTDPNPQEIYMTNVTENFNMWKNNMAEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTNLPKNSTNETNTNSSFMKEEVKNCSFNTTTELRDKKQKAYALFYKLDIVQMNDNRTYRLINCNTSVIKQACPKISFDPIPIHYCAPAGYAIFKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKNIIVHLNESVEINCTRPFNNTRTSIRIGPGQMFYRTGEITGDIREAYCEINRTRWNKTLSQVAKKLKEHFNKTIIFQPPSGGDLEITTHHFNCRGEFFYCNTTKLFNSTWNETGMSNDTITLPCRIKQIINMWQGVGQAMYAPPISGRINCVSNITGILLTRDGGNSSSKNETFRPGGGNIKDNWRSELYKYKVVQIEPLGVAPTKAKRRVVEREKRAITLGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLKAIEAQQHLLQLTVWGIKQLQARLLAVEKYLKDQKFLGLWGCSGKVICTTAVPWNSTWSNKSYNDIWNNMTWTEWEREISNYTEQIYSLLTESQNQQDKNEKDLLELDKWTNLWTWFGITNWLWYIRIFIMIIGGLIGLRIIFAVLSIVNRVRQGYSPLSFQIPTHHQREPDRPEGIEEGGGEQGRDRSVRLVSG",
    "response#3": "MRVKGTQMNWPNLWKWGTLILGLVIICSASDNLWVTVYYGVPVWKEANTTLFCASDAKSYDREAHNVWATHACVPTDPNPQEVFLENVTEKFDMWKNYMVEQMHADIISLWDQSLKPCVKLTPLCVTLNCSDATSNGTNSNTINSTRIKNSEEIKNCSFNTTTELRDRRKKEYALFYRLDITPLDNKKNSSENSSEYYRLINCNTSAITQACPKVTFDPIPIHYCAPAGYALLKCNNKTFNGTGPCNNVSTVLCTHGIKPVVSTQLLLNGSLAEGEIIIRSENLTNNAKTIIVQLNQSVEIVCTRPNNNTRKSIRIGPGQTFYATGDIIGDIREAHCNISADKWKETLQRVSKKLREHFPGKTINFTSSSGGDLEITTHSFNCRGEFFYCNTSGLFNGTYNPNVTESNSNSTNATITLPCRIKQIINLWQEVGRAMYAPPIAGNITCKSNITGLLLTRDGGTNDTSKTEIFRPGGGDMRDNWRSELYKYKVVEVKPLGVAPTGAKRRVVEREKRAIGLGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKSQQEIWDNMTWMQWDREISNYTKTIYELLEESQYQQERNEKDLLALDSWKNLWDWFNISNWLWYIKIFIMIIGGLIGLRIIFTVLSLVNRVRQGYSPLSFQTHIPSPREPD"
  },
  {
    "reference": "MADDKSQIKINFFTNEEDESLHTSDAPLYVPVSLKRYGLSEIVNHLLKKDGETDETKPVPFDFLIDGVLLRTSLEDYLVKNGLSSEASLNLEYTRAVLPPSFLASFNNDDWISSLDTINPLSASVKASNMSISQPKILSGSYDGIVRTYNMSGKVEKQYVGHSGPIRSVKWISPTRIVSSGNDRQVRLWKTSIDSNIEDDEEIEDGRTLAILEGHKAPVVSLAVEYQNNRILSAGYDKAIGFWSTNYREMTTIQPLEYDSNVISTSSKKRRKMALQDSTIRRRSPLSFLEGHSQPVEDVIFDFNDATVGYSVSQDHTIKTWDLVTSRCVDTRTTGYSLLSLLQLPSLNLLVCGSSARHINLFDPRVSSTTTEQTNNQKLIGHTNFVVGLSKCPTNDNMFASCSHDGTVKVWDVRSEKSLYTITREQNNAAKGQDKVFSVIWDNDIGIISGGQDKKIQINKGSDITK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.2. A protein that can perform ribonucleoprotein complex binding in nucleoplasm, preribosome, large subunit precursor, nucleolus to support maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA), maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA). The designed protein sequence is ",
    "response#1": "GKELKVACSQSCSRLMEKLISLSDMRQIRRLFNKFIGHFLHLVQHRFASHCCETLFINAAPGVTEKISKSKGRKAEVDEEDEPEPELSLAEMFMKVVEELEGNWGYLLTERFASHTIRVLLLVLAGEPVDLSSTDSVVASRKKERLGIVNTEASEENAVAERRKVPEVFEATLKKVMNDMVSGLDNTYLRALATHPVGNPVLQVLVFLELSHFGKASAKDPNSIIRRLIPDDDFGENAESSTFVRGLLYDPVGSRLLETIIKSMPGKLFKSLYKNIIRDRIGSLARNTTAGYVVLRTLERLGKDDLQDAMELIIPEIPGLIERSRLIVPKVLIERCLVRGVDTKPLSDALESAYDKNPARRLEQMLKLEPAASEEDSEEKQKQTGNNQTAAAEKLHGSLLAQTMLTASGPVSGLIYSSLLAQSAESLLRLAKDPTASRVLQQALTTPTSTSQFRRQFTTRFSGHLVELALDSSGSHVVDALWPATKDLFFVKERMAQELLKEELSLRDSFVGRL",
    "response#2": "GKELKVACSQSCSRLMEKLISLSDMRQIRRLFNKFIGHFLHLVQHRFASHCCETLFINAAPGVTEKISKSKGRKAEVDEEDEPEPELSLAEMFMKVVEELEGNWGYLLTERFASHTIRVLLLVLAGEPVDLSSTDSVVASRKKERLGIVNTEASEENAVAERRKVPEVFEATLKKVMNDMVSGLDNTYLRALATHPVGNPVLQVLVFLELSHFGKASAKDPNSIIRRLIPDDDFGENAESSTFVRGLLYDPVGSRLLETIIKSMPGKLFKSLYKNIIRDRIGSLARNTTAGYVVLRTLERLGKDDLQDAMELIIPEIPGLIERSRLIVPKVLIERCLVRGVDTKPLSDALESAYDKNPARRLEQMLKLEPAASEEDSEEKQKQTGNNQTAAAEKLHGSLLAQTMLTASGPVSGLIYSSLLAQSAESLLRLAKDPTASRVLQQALTTPTSTSQFRRQFTTRFSGHLVELALDSSGSHVVDALWPATKDLFFVKERMAQELLKEELSLRDSFVGRR",
    "response#3": "GKELKVACSQSCSRLMEKLISLSDMRQIRRLFNKFIGHFLHLVQHRFASHCCETLFINAAPGVTEKISKSKGRKAEVDEEDEPEPELSLAEMFMKVVEELEGNWGYLLTERFASHTIRVLLLVLAGEPVDLSSTDSVVASRKKERLGIVNTEASEENAVAERRKVPEVFEATLKKVMNDMVSGLDNTYLRALATHPVGNPVLQVLVFLELSHFGKASAKDPNSIIRRLIPDDDFGENAESSTFVRGLLYDPVGSRLLETIIKSMPGKLFKSLYKNIIRDRIGSLARNTTAGYVVLRTLERLGKDDLQDAMELIIPEIPGLIERSRLIVPKVLIERCLVRGVDTKPLSDALESAYDKNPARRLEQMLKLEPAASEEDSEEKQKQTGNNQTAAAEKLHGSLLAQTMLTASGPVSGLIYSSLLAQSAESLLRLAKDPTASRVLQQALTTPTSTSQFRRQFTTRFSGHLVELALDSSGSHVVDALWPATKDLFFVKERMAQELLKEELSLRDSFVGRR"
  },
  {
    "reference": "MNNNILVFDQVSNLNEPPNILLLSEEDHRVAKNAEIFIDKMITNLKFLSNSNSCDSSKKRKMIEQKTKCSNPRKNCGAKKLKTSPLPQPMTNLSHNHMFSIDEKPKRGRPPKPKPLCCQICLTNNTPYWRWSVIENNKIRVCNRCGQKIFKLEKSINEQLLFRIEIINLLNKIDDEKPIEK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein with zinc ion binding is needed to enhance regulation of DNA-templated transcription in a specific context. The designed protein sequence is ",
    "response#1": "MGTLEKKLDNLVNTILLKAENQHELLFGACQSDVKLTNTQEHILMLLSQQRLTNTDLAKALNISQAAVTKAIKSLVKQDMLAGTKDTVDARVTYFELTELAKPIASEHTHHHDETLNVYNRLLQKFSAKELEIVDKFVTVFAQ",
    "response#2": "MGTLEKKLDNLVNTILLKAENQHELLFGACQSDVKLTNTQEHILMLLSQQRLTNTDLAKALNISQAAVTKAIKSLVKQDMLAGTKDTVDARVTYFELTELAKPIASEHTHHHDETLNVYNRLLQKFSAKELEIVDKFVTVFAQ",
    "response#3": "MVDTESPLCPLSPLEAGDLESPLSEEFLQEMGNIQEISQSIGEDSSGSFGFTEYQYLGSCPGSDGSVITDTLSPASSPSSVTYPVVPGSVDESPSGALNIECRICGDKASGYHYGVHACEGCKVEGTCNRPLVQPCPRSHDLTAHACFPLQTLAIRFGRMPRSEKAKLKAEILTCEHDIEDSETADLKSLAKRIYEAYLKNFNMNKVKARVILSGKAGNNPVGVCSSDSPTSASRVSGITGVSHRAQPTKPSSNTITLLKYGGTLEAEYLVITTLKPLSLLSRLDRPGLLNVGHIEKMQEGIVHVLRLHLQSNHPDDIFLFPKLLQKMADLRQLVTEHAQLVQIIKTESDAALHPLLQEIYRDE"
  },
  {
    "reference": "MNKKFKCIGIVGHPRHPEALATHEMLYHWLKSKGYSVIIDRQVAKDIGLKDAQTGGLTEIGKQADLAVVVGGDGNMLGAARVLSRYDIKVIGINRGNLGFLTDLDPDNALQQLSEVLDGEYRNEHRFLLEAQVRRNGQKPRISTAINEVVLHPGKVAHMIEFEVYIDERFAFSQRSDGLIIATPTGSTAYSLSAGGPILTPNLDAIVLVPMFPHTLSSRPLVISSESSIRLKFSQNSNDYEVSCDSQIVLPIQDGEDVIISRSKQKLNLIHPQDYNYFNTLSTKLSWSKKMF",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein with ATP binding functions is required to enable phosphorylation, NADP biosynthetic process, NAD metabolic process.2. The protein must exhibit the following characteristics: Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.. The designed protein sequence is ",
    "response#1": "LNGL",
    "response#2": "MDLN",
    "response#3": "MERI"
  },
  {
    "reference": "MPPSVTVAVIVVAAGSGERLGSGLPKAFVQVAGTTLLEHSLEPLTTLGKQAEIVVVAPSSWMEPARELAXRVXGTKASVAVVSGGATRTXSVRAGLXAVSXKAQIVLVHDAARAFTPAEVFDRVIEAVEAGSAGVIPVLDVTDTIVPVDDSSLTXAAXDRTKLRAVQTPQGFDAATLRASYDAFSGXATDDAEVLRHAGHEVMAVPGHPDSHKITYPTDLVAAERTFGHGAELRVGTAVDVHQFDDDSALVLGGMNFDGPGLSGHSDGDVILHVITDALLSAAGLGDLGTHFGTDRPXFEGAKSEVFVTEAXRLLTEAGFSPRSVSVQYIGNTPRLGGHREAMGQHXSALVGCPVHVSATTSDGLGLTGRGEGAAALATAXXEYQPGAXDPSG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Ideal protein should have a binding affinity for key enzymes in the isopentenyl diphosphate biosynthesis via DXP pathway, isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6, Isoprenoid biosynthesis pathway to enhance the overall efficiency of the pathway.2. The stability of the protein-a divalent metal cation complex should be optimized to maximize enzymatic activity.3. The designed protein should have a binding site that is specific to the target 4-CDP-2-C-methyl-D-erythritol 2-phosphate, a divalent metal cation ligand.4. For general function, the protein need meet that Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D- erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C- methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF). The designed protein sequence is ",
    "response#1": "RVGIGTDVHAFGGDGVLWLAGLEWPGERALSGHSDGDAVAHAIVDALLAAAGLGDIGTHFGTDQPQYAGAHAEAFLARTAEILREEGWRAGNVSVQVQANRPRFAARRVEAERVLSDALGGVPVSVSATTTDGLGFAGRTEGVSASAVALRVGTGYDVHRLVAGKPLWLCGVEIAHERGLSGHSDADVAIHALVDALLGALAEGDIGSHFPPSDPQWKGAPSHTFLTFARDRVAARSGEIAHVDVSIICEAPKIGPHREAMRARLAELLEIDPGRVSVKATTTERLGFTGRREGIAAQAVATE",
    "response#2": "RDDFTDDAALAEWVGLTVATFEGDAANMKLTTPEDFVREEARLAAALGDIRTGTGYDVHAFGEGDHLMLCGVKVPHNCGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPSDPQWKGAASDKFLKYAVDRVTARGGRVANLEVTMICQQPKIGPLRDQMRARIADITGVAISRIAVKATTSERLGFTGREEGIAATASATGSDGDAVAHAIVDALLGRDDFTDDAALAEWAGMTVSTFAGEAGNVKLTTPEDFLRAEATSLSELGDIRTGFGTDVHQFTDGDHIWLGGVKIPHVRGVTGHSDADVVLHSLVDAILGAISDGDIGVHFPPSDPQWRGASSDQFLAFAVERVRARGGLIAHLDITVVCEAPRVGPHRDAMRARIAEIAGVSPERVAIKATTSEKMGFTGRGEGIVAFANATQEFVDPDSLARVGMGFDVHRLVVNRPLVLGGAIVPFDKGCEGHSDADVLVHAIIDSLLGAAGAGDCGRHFPDTDYRYKDISSLILLKQTGQIIRNAGFSIENIDAVVVAQAPQLINHIPLMEENIASTLGIEKALINVKAKTTEGLGYCGRGEGIAAYATALLVT",
    "response#3": "IRTGTGYDVHAFGEGDHLMLCGVKVPHNCGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPSDPQWKGAASDKFLKYAVDRVTARGGRVANLEVTMICQQPKIGPLRDQMRARIADITGVAISRIAVKATTSERLGFTGREEGIAATASATIRDAATLTEAIEERDVHAGLTDIRTGIGYDIHAFDTGGDHVWLGGIKIAHARKLAGHSDADVALHALTDAILGALADGDIGVHFPPSDAQWRGASSDTFLKFAVERVRARGGQVSHLDVAIVCEAPKVNPHRDTMRARIAAIAGLSIDRVGVKATTNEKLGAIGNGEGIAAYATATRTGNGFDVHAFGPGDHVILCGLRLPHDRGLRGHSDADVGMHALTDALYGALAEGDIGRHFPPSDPQWKGADSRLFLRHAAERVRARGYRLSGADVTLICERPKIGPHVSAMQAELAVELGIEPDRVSVKATTSERLGFTGREEGIAALATATSVLPVSVRTGNGYDVHRFGDGDHVMLCGVKVPHGRGLQGHSDADVGMHALTDAIYGALAAGDIGRHFPPSDQQWKGADSRVFLAHAAGLARDGGWRLTHADVTLVCERPKVGHHADAMRAALAEVMGIEVERVSVKATTSERLGFTGREEGIAALATATLVPVEAL"
  },
  {
    "reference": "MSDTGRKIMRVLSGESLSPPPLWLMRQAGRYLPEYRETRAKAGSFLDLCYTPEHAVEVTLQPIRRYGFDAAILFSDILVIPDAMKRNVRFTEGHGPEMDPIDEAGIGSLNGEEVVDYLRPVLETVRRLREELPAETTLLGFCGAPWTVATYMIAGHGTPDQAPARLFAYKHARAFEHLLMLLADVSADYLVAQIDAGADAVQIFDSWAGVLGEKEFEAFAIRPVARMIASVKSRRPHARIIAFAKGAGYQLKTYRQKTGADAIGLDWSVPLAFAAELQKDGPVQGNLDPMRVVAGGRALEEGIDDILQHLGNGPLIFNLGHGITPQADPEHVRLLVDRVRGGA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III..2. A protein that can modulate the overall flux of metabolites through the coproporphyrinogen-III from 5-aminolevulinate: step 4/4 pathway, enhancing or suppressing its activity as needed.3. The protein should have a specific function in protoporphyrinogen IX biosynthetic process and should be localized to cytoplasm in order to carry out this function efficiently.4. The substrate binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "KNDRFLRALMRQPVDRTPVWMMRQAGRYLPEYRATRAKAGDFLSLCKNTPLACEVTLQPLERYPLDAAILFSDILTIPDALGLGLYFETGEGPKFRKVIRSEADVDALPRMNAESDLDYVMNAVSTIRRELNGRVPLIGFSGSPWTLATYMIEGGSSKTFSEAKKLMYGQPEVMHRLLDHLADSVIDYLNGQIKAGAQAVQIFDTWGGVLSSWAYEEFSLRYMTKIVDGLIRESEGRRVPVIVFTKNGGQWLESIADCGADAVGLDWTTDIGNARARVGDKVALQGNMDPAMLYAPKARIRQEVADILKRYGSGSGHVFNLGHGITPDVDPEHAGAFIEAVVKR",
    "response#2": "MKNDLFLRVLRGEKVERTPVWIMRQAGRYLAEYRKLRAKVPHFLDVCRNPELACELALQPLQRFPLDAAILFSDILTIPDAMGLGLSFVAGEGPQFAKPLRSSAAIESLTLPPSGSLDYVFAAVSATKRALNDRVPLIGFAGSPWTLAAYMIEGGASKDFAFAKSFAFSQPQAMDVLLTQLTCAITDYLRGQIKAGAQAVMIFDSWGGVLPYWAYEQFSLPYLKKIVAAVHTVAPVIVFTKGGGLWLAAQKTIGAAALGVDWTVSLAAARKIVGESIVLQGNLDPTFLMTDPKTIRQAVAKTLADYGNGHRYIFNLGHGITPNASPDNVAAMIEAVHRL",
    "response#3": "MKNDLFLRVLRGEKVERTPVWIMRQAGRYLAEYRKLRAKVPHFLDVCRNPELACELALQPLQRFPLDAAILFSDILTIPDAMGLGLSFVAGEGPQFAKPLRSSAAIESLTLPPSGSLDYVFAAVSATKRALNDRVPLIGFAGSPWTLAAYMIEGGASKDFAFAKSFAFSQPQAMDVLLTQLTCAITDYLRGQIKAGAQAVMIFDSWGGVLPYWAYEQFSLPYLKKIVAAVHTVAPVIVFTKGGGLWLAAQKTIGAAALGVDWTVSLAAARKIVGESIVLQGNLDPTFLMTDPKTIRQAVAKTLADYGNGHRYIFNLGHGITPNASPDNVAAMIEAVHRL"
  },
  {
    "reference": "MAKKIIAKIKLQLEAGKATPAPPVGPALGQHGVNIMDFCKKFNAVTADKPGMVFPAVITVYADRSFTFELKTPPASFLILKAAGIKKGSGEPNKVKVGKITKAQVEEIAKIKMPDLNARTLEAAMKIIEGTARNMGVEVVD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors..2. The protein must have high specificity towards large ribosomal subunit rRNA binding, structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "MPQVGNALPLRFIPVGTVVHAVELEPKKGAKLARAAGTSAQIQGREGDYVILRLPSGELRKVHGECYATVGAVGNADHKNIVLGKAGRSRWLGRRPHVRGAAMNPVDHPHGGGEGRAPRGRPPASPWRT",
    "response#2": "MLFDLVLTSAGEKKVNVIKVVREITGLGLKEAKAAVDGAPATLKEGMSKEDGDEAKTKLEEAGASVELK",
    "response#3": "SIQVGNALPLRFIPVGTVVHAVELEPKKGAKLARAAGTSAQIQGREGDYVILRLPSGELRKVHGECYATVGAVGNADHKNIVLGKAGRSRWLGRRPHVRGAAMNPVDHPHGGGEGRAPRGRPPASPWGWR"
  },
  {
    "reference": "MEQHLYTVAQIRAIEEAAYAGLEPGALMRRAGRAAADHALELLEGRRDRPVLVLAGPGNNGGDALELAANLAEAGVDATVLHLPGRREASAETAAAYERARAGSVGWIDMLPPGADWALVVDGLFGIGLERPLEGEARELVEGVNALRCPMLALDVPSGLDADTGAVVGPDGVAVRATHTITFLGDKPGLHTGDGRDYAGEVRVDMLGVATADATAEAKADGETPLPRAAARLGGVDLFAGQLAARRHNSHKGSFGDVAVVGGAHGMAGAPVLAARGALFAGAGRVYVAALDHPPGYDSTQPEIMFRLADEFEFDRRTLVIGPGMGGSANAMRLLAKALDGDGPLVVDADALNLIGASPDLQSRLAQRKAPAVLTPHPLEGARLLGMTTAVVQADRLEAAREMALRTNAVVVLKGSGSVIARPDGEVVVNPTGNAGLATGGSGDVLAGICGALLAQGWPAWEAAAGAVWIHGAAADLLVEQGVGPIGMTAGELPAAARAVINRLVREAGAR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be optimized for ATP binding, NADHX epimerase activity, NADPHX epimerase activity activity in order to improve nicotinamide nucleotide metabolic process.2. The protein should have a high affinity for (6S)-NADPHX, K(+). The designed protein sequence is ",
    "response#1": "PTAHKGSFGQVLVIGGDLGTGGAALLSAEAALRCGAGMVTLATRPEHVSASLVRRPEVMCNGVESTYSLTALLERADVLVVGPGLGQAPWGRSLISAAAQREQPQVWDADALNLLATGAVELPSGCLITPHPGEAARLLKCSVGEVQADRPAAARALAQRYGCVALLKGAGTLVAAPDGRLALCDRGHPAMASAGLGDVLAGVLGALLAQQLAPFEAACLGAWLHAAAGERLGPLGRGLAASDLIPVIE",
    "response#2": "PDSHKGSYGHLLVVAGSRGKTGAAILACKAGMRAGAGLVTLAAARSLNEIFAGSMMEAMTEPLRDNADEEILPPSDQEWRRLLDKKSALVFGPGIGVNESTRAALWWLLRNLDLPWVIDADGLGLLAGNVGRLRGAKRPPVLTPHPGEMARLIGTEAAPSTALRTGAVNNDRVGVARSFAREQRCYVVLKGARTVIATPDGKAFINPTGNPGMASGGMGDVLAGILGGLLAQGFKIDDALKLGVFLHGFVGDRVAALKGEMGLIASDVIY",
    "response#3": "DKYTQGVTGVLAGSAAYPGAAILCAGAAVAATSGMVRYAGSAAAEVVDHWPEVVIAPSAAAAGRVQAWAVGPGLGTDETAAAALWFALDTDLPVIVDADALTMLAAHPDLVAGRTAPTVLTPHAGEFARLAGEPPGADRVGAARALADRFGATVLLKGHVTVIAEPGGGSVYLNPAGQSWAATAGSGDVLSGMIGALLAAGLPAGEAAAVAVFVHTRAAALAAADPGAGTAPASAI"
  },
  {
    "reference": "MSGFSVYIAVELAIAVLAILGNVLVCWAVWINSNLQNVTNYFVASLAAADIAVGVLAIPFAVTLSTGFCAACHSCLFFACFVLVLTQSSIFSLLAIAIDRYIAIRIPLRYNGLVTGTRAKGIIAVCWVLSFAIGLTPMLGWNNCSQQESRNSSQVCGEGQVACLFEDVVPMGYMVYYNFFACVLLPLLLMLGVYLRIFLAARRQLKQTESQPPPPGERPRSTLQKEVHAAKSLAIIVGLFALCWLPLHVINCFTFFCPSCGHAPPWLMYLTIVLSHGNSVVNPFIYAYRIREFRHTFRRIVRSHVLRRRRRREPFKAGDPGARALAAHDPERVGLRLNGHPPGGVWANGSAPRPDGPALALPSAGGARGSQRDARVPDVQLQGRRPESPGLGGPRGRAGVS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. A protein that can perform G protein-coupled adenosine receptor activity, enzyme binding, lipid binding, G protein-coupled receptor activity, identical protein binding in postsynapse, plasma membrane to support positive regulation of synaptic transmission, GABAergic, synaptic transmission, dopaminergic, eating behavior, response to inorganic substance.3. The protein must exhibit the following characteristics: Receptor for adenosine; The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.. The designed protein sequence is ",
    "response#1": "LSTLQLMYTVGYSLSLISLFLALTLFLFLRKLHCTRNYIHMNLFASFILRALVVLVKDMVFYNSYSRRPDSESGWMSYLSEISASCRSVQVLLHYFVGTNHLWLLVEGLYLHALLEPTVLPERRLWPKYLVVGWAFPMLFVIPWIFVRASLENTGCWAVNENKKIWWIIRGPILLCVTVNFFIFLKILKLLISKFRAHQMCFRDYKYRLAKSTLLLILLMGVHEFLFTFFTDDQVQGFSRLIRLFIQLTLSSFHGFLVALQYGFASREVKAELRKTWGL",
    "response#2": "EQSQQQENLMLSILPKHVADEMLKDMKKDESQKDQQQFNTMYMYRHENVSILFADIVGFTQLSSACSAQELVKLLNELFARFDKLAAKYHQLRIKILGDCYYCICGLPDYREDHAVCSILMGLAMVEAISYVREKTKTGVDMRVGVHTGTVLGGVLGQKRWQYDVWSTDVTVANKMEAGGIPGRVHISQSTMDCLKGEFDVEC",
    "response#3": "EQSQQQENLMLSILPKHVADEMLKDMKKDESQKDQQQFNTMYMYRHENVSILFADIVGFTQLSSACSAQELVKLLNELFARFDKLAAKYHQLRIKILGDCYYCICGLPDYREDHAVCSILMGLAMVEAISYVREKTKTGVDMRVGVHTGTVLGGVLGQKRWQYDVWSTDVTVANKMEAGGIPGRVHISQSTMDCLKGEFDVEC"
  },
  {
    "reference": "MASEQRWIPLESNPKVLNKYVHNLGMDAGWNFVDVFGLDPELLAMVPRPAAALVLLFPDDKETVNQLIGEYQSDYPDSLYYTKQTIGNACGTVAIVHALANNENVIPFDAAKHFKTFLEKTKPLNPEERAKHLEQDNLMGAAHGDCAQEGDTQAPSQDEHVKSHFVALVHCNGTLYELDGRKEAPVVHGTTSADTFLEDAAEVVKKFMARDPEI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for stability and expression in cytoplasm where it will participate in ubiquitin-dependent protein catabolic process.2. The Proton donor, Nucleophile of the protein must be engineered to enable effective catalysis of Thiol-dependent hydrolysis of ester, thioester, amide, peptide   and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-   residue protein attached to proteins as an intracellular targeting   signal)..3. The protein must exhibit the following characteristics: This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.; Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. The designed protein sequence is ",
    "response#1": "WRPLENTPEVLEPYLQKIGVQDASVFDLFSLEEIPEYIPRPVHALLFVFPSSGTKTIYKGSRILPKDSDKVLWYPQTIPNACGTIGLLHAVSNGELRRKVNENDFIKSLIRTAEGSSIEERAKLIEDSKELEALHAAFAGPPLEVEGSEEDVETDLHFICFVKGKSKDDNHFYELDGRQEGPVQHSEIESDLLNAEVLSVIKNYIH",
    "response#2": "QRWIPLESNPKVLNKYVHNLGMDAGWNFVDVFGLDPELLAMVPRPAAALVLLFPDDKETVNQLIGEYQSDYPDSLYYTKQTIGNACGTVAIVHALANNENVIPFDAAKHFKTFLEKTKPLNPEERAKHLEQDNLMGAAHGDCAQEGDTQAPSQDEHVKSHFVALVHCNGTLYELDGRKEAPVVHGTTSADTFLEDAAEVVKKFMARDWRPLENTPEVLEPYLQKIGVQDASVFDLFSLEEIPEYIPRPVHALLFVFPSSGTKTIYKGSRILPKDSDKVLWYPQTIPNACGTIGLLHAVSNGELRRKVNENDFIKSLIRTAEGSSIEERAKLIEDSKELEALHAAFAGPPLEVEGSEEDVETDLHFICFVKGKSKDDNHFYELDGRQEGPVQHSEIESDLLNAEVLSVIKNYIQSIDSPFFSLVAIG",
    "response#3": "WRPLENTPEVLEPYLQKIGVQDASVFDLFSLEEIPEYIPRPVHALLFVFPSSGTKTIYKGSRILPKDSDKVLWYPQTIPNACGTIGLLHAVSNGELRRKVNENDFIKSLIRTAEGSSIEERAKLIEDSKELEALHAAFAGPPLEVEGSEEDVETDLHFICFVKGKSKDDNHFYELDGRQEGPVQHSEIESDLLNAEVLSVIKNYIQSIDSPFFSLVAIS"
  },
  {
    "reference": "MAGQAASKRRLISRSLQSADICLQHQDYGTAYAHLLLVLTLAPEQKEALKEMFQYSLFKWAEELYALNRSQDLFNCYEQALELFPIDDVICNSMGEHLFRLGFRDEAAGYFYKALKLNPSSAEAKENFYRVANWLIERWHFIMLNDTKRNLMYRAAIQNAIQNGCKTVLDIGTGTGILSMFAKKAGAPFVYACELSKTMYELACEIVTANQMDGHIKLLHMKSHDIQIPEHIPERVSLVVTETVDAGLFGEGIVETLIHAWKNLLLQPKPKDGRVEAYGKVIPSSAVIYGMAVECPEIRRHYSVGVTEVAGIKLGDAVKFCSPIHSSHGPDDVTEPYTTEKMSRVPGGYKALSQPFQVMTVDFNSLQALEYIASGKSNRISVPVYQQGQFDCFITWFALQLDNEHSLSTEPSEETCWEQAVFPVQKLPDEGCLVNTGDTIVVDVSCPDCYLRLDLSTIVLSESSCDQTENMVMGNETDICDALANLHTTTNKGNMQELCILEPGEIALLNNAVYHESFMAAISKVIGSLELKESCSVVRNSQEQDVNFAQPVSEDRLHVLDVSEGFSILPLIAAKLGKVKAFSSVEKEQHRVALEKLSVINDLNNNESLEFCLSQLETDDGAAQKPKSDKMWSIIILDVIETCGLIRQDLLEKAAIARCLLEPGGKIFPHAVVMQGMLIESKTLLHEGSVQGNEPTLGFLIAPFINRFKVPAHVFLNLSTVPCIPLSEQFELLRLDLMNPCSNNQSSSVMRIKVNICRSGQVTAVTFW",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit protein-arginine omega-N symmetric methyltransferase activity.2. The protein should have a low Km value for L-arginyl-[protein] + 2 S-adenosyl-L-methionine = 2 H(+) +   N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-   homocysteine.3. The protein must exhibit the following characteristics: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA).. The designed protein sequence is ",
    "response#1": "FDFLLAPLVDPDYRPTPGSVLPVAASDLVLGPAQWSSHIVGKISEWIDLDAEDEQLRLDSEITLKQEIAWASHLSLQACVLPPPKRSSCANYARVVNHILQGLTNLQLWLRIPLEKSEPMDEDHDGAKDNSDMSDTVDSWEWWNSFRLLCEHSSQLCVALDVLSTLPSMNSLGRWFGEPVRAAILQTNAFLTNARGYPCLSKRHQKLLTGFFNHSVQVIISGRSNHNVSQGGVLSGDENHTEDTAVRHALSPYLDYIAYIYQES",
    "response#2": "FDFLLAPLVDPDYRPTPGSVLPVAASDLVLGPAQWSSHIVGKISEWIDLDAEDEQLRLDSEITLKQEIAWASHLSLQACVLPPPKRSSCANYARVVNHILQGLTNLQLWLRIPLEKSEPMDEDHDGAKDNSDMSDTVDSWEWWNSFRLLCEHSSQLCVALDVLSTLPSMNSLGRWFGEPVRAAILQTNAFLTNARGYPCLSKRHQKLLTGFFNHSVQVIISGRSNHNVSQGGVLSGDENHTEDTAVRHALSPYLDYIAYIYQED",
    "response#3": "FDFLLAPLVDPDYRPTPGSVLPVAASDLVLGPAQWSSHIVGKISEWIDLDAEDEQLRLDSEITLKQEIAWASHLSLQACVLPPPKRSSCANYARVVNHILQGLTNLQLWLRIPLEKSEPMDEDHDGAKDNSDMSDTVDSWEWWNSFRLLCEHSSQLCVALDVLSTLPSMNSLGRWFGEPVRAAILQTNAFLTNARGYPCLSKRHQKLLTGFFNHSVQVIISGRSNHNVSQGGVLSGDENHTEDTAVRHALSPYLDYIAYIYQPL"
  },
  {
    "reference": "MLPVVTPIEMKAIDAQAAESEEVLIQRAGYAVANSALRMLGGSYGRRVNVIIGKGNNGQDGQVAARLLQRRGVRCKIYRPGPEILAEADLVIDAAYGTGFRDEWEPPPQPRCPVLAVDIPSGVDGLTGEDHGSLQAERTITFGALKPGLLLEPGASRAGQLEIADLGLNVGSSRCNLITDHDLRMPLSQRTATDHKWTNAVRLIAASPGMIGAAQLAASGALRAGAGMIVVSSPTTNLQASDMPPEAVTRQLTNNDLVSEVLKDLDRFAALLIGPGLGTDPDTIAATAELIERSPIPTVVDADGLKAVAAHPSCLQRRVAATVLTPHDGEFEKLTGSPPSADRCAAVREAAKDSAVLLKGPTTLVSDVNGDLIFVRNANQRLATAGTGDVLTGIIGAIIRNSPIHLAAAAAAHWHGQASQLAQPHMTASDLPLLLDPARSAMLSK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must possess metal ion binding, NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, ATP binding to support nicotinamide nucleotide metabolic process under certain conditions.2. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.3. The protein should have a high affinity for AMP, (6S)-NADPHX.4. The protein should have a low Km value for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.5. For general function, the protein need meet that Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP; Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.6. The protein contains novel YjeF N-terminal domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MREIDRLTTERYATPSLLLMEAAATATARSIAARFPGGLADKTARVICGRGNNGGDGAALARALWLAGVRTDVVLIGRVEEMKNDARTNFEIVRHLASFEAGSRQSRPPLTFVECESITDWEDIARPRHTYQIIVDALFGTGLSRPLEGIALMVVEHLALIRSARERAHEADPLIVSMDIPSGLNADGSEVTGEVVHADMTVTFTAPKPANVLPPASHYNGQLIIAGIGSPDSYKNTHGHALLIAGSRGLAGAAVLCANAAMSSGAGLVTVATPSSAQSAVAAQAMPEVMTAALPETTNGAVSFDAVERVLELRERASVIAIGPGLTSADETTRRFVREIIERRSTPVIIDADGLNALAPWPDDLHGTEEHPLILTPHPGEMLRLLGTEDHAVLSDRVTAAREFATAHSLILVLKGTRTLVAAPDGRVFINPTGNAGLGTAGAGDTLTGIITGLNAQSYATLKEEADALSATIAAVYVGGLAGDMAARESGMRTMTASDIL",
    "response#2": "MMITDINCECLGLSRLCLMESAGKSLAEEVGKIAVYTFSKPVKIAIFTGSGGNGGDGFVAARYLLNRGYDVDIYMLTDKDNIHSDNAKTNFEIITNMEPRLSHLTVHYLDTIEDVNNCDIAQSESFSEFIIVDAILGTGIKGELRPKVKRAVEVINKSNGLTVSVDVPSGMDPLTGEVSDVAVEPHYTISFHRVKTGIHLAGEDKVGGIVTCDIGIPIEAERFTADGTCLPKREKTAHKGLFGHVLVAGGERGFSGAVQMAASAALRVGAGLVSLATRSEHAALLNLVRPELMVHGIDKRVDLAPLLKKSSVAAVGPGLGQTVWAQNLLAELFDTAMPLVVDADALNLLAKEPFKRDNWVLTPHPGEAARLLGITIKEIESDRFSSVKALQKKYGGVVVLKGAGTLIFDGSEMVINTTGNPGMASGGMGDVLSGVIAALLAQGLSLSQSARLGVWLHGKAGDIAAKEGERGLLASDLLRPLRVLVNS",
    "response#3": "MREIDRLTTERYATPSLLLMEAAATATARSIAARFPGGLADKTARVICGRGNNGGDGAALARALWLAGVRTDVVLIGRVEEMKNDARTNFEIVRHLASFEAGSRQSRPPLTFVECESITDWEDIARPRHTYQIIVDALFGTGLSRPLEGIALMVVEHLALIRSARERAHEADPLIVSMDIPSGLNADGSEVTGEVVHADMTVTFTAPKPANVLPPASHYNGQLIIAGIGSPPALIGALPRRRIDAHKGDSGHVMVLGGDSGMAGAALLCARAALRAGAGLVSVGTRAAHAAALVAAQPECMVRAVEAASELDPLLERATVIALGPGLGQGDWGHALFERVLVAHRPCVLDADALNLLAQAPRACAQAVLTPHTGEAARLLGVSTTEVQRDRYTALAHLVQRYQCPVVLKGAGSLVGAPGELPRVIDAGNPGMAVGGMGDLLAGVIAALIAQGLEPFDAAVLGALAHAAAGDLAAAEGERGLLPSDLLPLLRQVLNG"
  },
  {
    "reference": "MARTSSLMVTSEAFPLAKSGGLGDAVTGLARALSRAGVDTGILMPAYRGVLDRVVGIRHIAHLAGMPGGEATLVGALCPESGLPFYLLCNPGLYDRDGLYLDEQGQPYADNAVRFAALAHAAMRVAAGLPGMRRPDILHAQDWHAGLVPLLVRAAGLRYVKTVLTVHNLAFQGSFELECAGQIGVPGAYCTDDGARLHGRINFMKAGLRYADRITTVSRNYAREIMTPEFGCGLDPLLRARAADLAPIPNGIDDALWNPASDPHLGHLCYSARNPARKSRAKAALQKAFGLHPDQGAALLAMGSRLTSQKMADVAVEALPDALHRHPSLQVAIIGRGERQYEIELEALAARYPGRCAVRIGYDETTAHRLHAGADMLLHGSRFEPFGLTPLYAMRYGAVPIASRVGGMADTIVDPGEREGSQAMLAATGILFDGDGAPDMGHAIDRALRLYAQPAIWRSMQRNGMACEFGWNTAAQPYLDLFENMVDAPRRGRVPAPTIRPAPLPAYARSSLDGMPAPAAY",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein should be able to manipulate the activity of enzymes in the glycogen biosynthesis pathway, either by activating or inhibiting their function.2. The ADP-alpha-D-glucose binding site should be located in a region of the protein that is accessible to the ligand.3. The protein must exhibit the following characteristics: Synthesizes alpha-1,4-glucan chains using ADP-glucose..4. The designed protein should have alpha-1,4-glucan synthase activity, glycogen (starch) synthase activity to facilitate glycogen biosynthetic process.5. The protein should have a fast turnover rate for [(1->4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1->4)-   alpha-D-glucosyl](n+1) + ADP + H(+). The designed protein sequence is ",
    "response#1": "AMLLAGGKGSRLSGLTKNMAKPAVSFGGKYRIIDFTLSNCSNSGIDTVGILTQYQPLELNSYIGIGSAWDLDRYNGGVTVLPPYAESSEVKWYKGTASSIYENLNYLNQYDPEYVLILSGDHIYKMDYGKMLDYHIEKKADVTISVIEVGWEEASRFGIMKANPDGTITHFDEKPKFPKSNLASMGIYIFNWPLLKQYLEMDDQNPYSSHDFGKDIIPLLLEEKKKLSAYPFKGYWKDVGTVQSLWEANMDLLLPERDDVPLFGLVGRLVKQKGIDILAEAIHRILALDVQVVMLGAGEPWSHFYFGDVRNEYPEKFGLYIGYNNGLSHRIEAGSDFFVMPSAFEPCGLNQMYSLRYGTLPIVRATGGLDDSVENFDEQNLTGNGFKFWSHDADALFDTVGWVNVEKGDLPLIGLIGRLTYQKGFPTFLQAFLERRHLPHRYVVLGSGDPETENAFFHLSDMMPDVFYFYKGYNESLAHQIEAASDFFLMPSLFEPCGLNQMYSHVYGTIPIVSRVGGLRDTVDESIDIQFKTGIVFEPNDKSSLGYALEGPLCVLVSRLTDQKGIDLLLDAMHVVLERGGQVAVLGSGAPDLEVCLLERADAEPNLAVKIGYDEALSHRMMAGGDCILVPSRFEPCGLTQLYGLRYGTLPLVALTGGLADTVINASPAALARRVATGIQFSPITAEALANAFSRLCDLYADRKTWLHNAFEGTPVVGVILNFDEFQGAELLQQSLEQLAALDIQLIISGSGDKKYEKVFQDFALEHPEQVSVHGEYSDSFFHLAIAGLDILLMPGMIESCGMIQMFAMNYGTIPVAYAGGGIVETIEERDEESGSGFIFHDYSAESLVGKLEEALAC",
    "response#2": "AMVLAGGKGERLAPLTLRRPKPGVAFGGKYKIIDFVLSNMFNSGIKKVYILTQYRAYSLMKHIRESWGKWAGLGEFFVAISPETSSESEEWFKGTADAINHYLRFIESSDADYVAIFGGDHIYRMDVSQMIGYHRRNRADITIAALEVPVEEARRFGVFCVDDDNRVTAFEEKPANPVTIPGRETCFASMGNYIFSTRRLIEVLQEGKKLHADLDFGKHVIPMMLAKKDRVFAYNFNDNLIPGMKPEERGYWKDVGTIDSYYEANMEDVPMFGMINRLVDQKGLDIMAEIMDYVSLFDIQFVLLGTGEEKYENMFKKLGEKYPEKYSINLKFDIVLAQKIYAGCDMFLMPSRYEPCGLGQMYSLRYGTIPIVRYTGGLADTVKEYNPETKEGNGFGFEGFDPAHLLKAMARAIYFYNSKELEVNPDTYLLGMVSRLVDQKGVDLLLQVTERLLAYTDSQIVVLGTGERGLESGLWQLAIQNPGRFSVFLTYDDDLARLIYGGSDAFLMPSRFEPCGISQLLAMRYGSVPVVRKVGGLVDTVPSYDPIHQTGTGFCFDRFFLGEDENIPMIGIVSRLVSQKGLDLISCVLEDLLQDGIQLVVLGTGDAKYENMFKYFAWKYPNKLSANIQFNNSLAQKIYGASDMFLMPSKFEPCGIGQLIALRYGSLPIVRETGGLKDTVRPFNPITGEGNGFSFTNYNAHDMLHVIEGTPVVGVILNFDEFQGAELLQQSLEQLAALDIQLIISGSGDKKYEKVFQDFALEHPEQVSVHGEYSDSFFHLAIAGLDILLMPGMIESCGMIQMFAMNYGTIPVAYAGGGIVETIEERDEESGSGFIFHDYSAESLVGKLEEALAC",
    "response#3": "AMLLAGGKGSRLSGLTKNMAKPAVSFGGKYRIIDFTLSNCSNSGIDTVGILTQYQPLELNSYIGIGSAWDLDRYNGGVTVLPPYAESSEVKWYKGTASSIYENLNYLNQYDPEYVLILSGDHIYKMDYGKMLDYHIEKKADVTISVIEVGWEEASRFGIMKANPDGTITHFDEKPKFPKSNLASMGIYIFNWPLLKQYLEMDDQNPYSSHDFGKDIIPLLLEEKKKLSAYPFKGYWKDVGTVQSLWEANMDLLEPVEVIDIDDPRELENAKVEDSLVVDGCFVDGTVKHSILSTGAQVRKDAVIEDSVIMSGAIIGQGAKIKRAIIGEGAIIAEGVEVRAPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPAFEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGTLPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWYTGEQAELVEGTPVVGVILNFDEFQGAELLQQSLEQLAALDIQLIISGSGDKKYEKVFQDFALEHPEQVSVHGEYSDSFFHLAIAGLDILLMPGMIESCGMIQMFAMNYGTIPVAYAGGGIVETIEERDEESGSGFIFHDYSAESLVGKLEEALACGR"
  },
  {
    "reference": "MDSSPVTYSGEPPYKLRRLSPSYPYVSKLRERCASKIETLSEGSARDSLEEEDVSEAMATGAFLATRLYLPSVLPQRITTLTFLDHFKKSRPLPNSDKRLNPIFYRLAYIRDLVGEMELEGIVERGTASRLLGASSPAGFVAGTYTHARDLSKTMSLASVRDAVLAIEAQTRDQSESQLWALLRRGLATASTMKWGALGPQYHPQWCEVSTNAKGIPNNPALQFGQTNERTARSLISALYVARSEAATPDLLVDPGCGQCFVFDESASVPGDAYACGLLMDARTGVVGASLDMLVCDRDPSGVLSPHSTQTTLDFFEIKCRAKYLFDPDLFSPVATAYANLLKHRTAVCLRKFLRSIKNPAVEYFASTSVPGATEALITCNSSWKPREVNETNRRCGDFDRDHIALNLDASSDVWLFSEPDLESETITPARWDTGELALSVPVFANPRHPNFKQILVQAYVLSGHFPDHQLRPFLVTFIGRHRKRCEEGKTFTICDRPEGSPYNLNEVVHSSCAIPILLFVTPVIVDREGCWEDIEIESLTAFNKTADAIWDSDSPADVSEPTSS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Additionally, forms a recombinase with the major DNA-binding protein, which displays strand exchange activity.; Plays a role in processing non linear or branched viral DNA intermediates in order to promote the production of mature packaged unit-length linear progeny viral DNA molecules; Exonuclease digestion of DNA is in the 5'-> 3' direction and the products are 5'-monophosphate nucleosides2. The protein can be targeted to host cell nucleus and perform endonuclease activity. The designed protein sequence is ",
    "response#1": "SMSPQTETKASVGFKAGVKEYKL",
    "response#2": "SCGTV",
    "response#3": "LHHHHHH"
  },
  {
    "reference": "MTKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFISAKMRKVNNFTTGQIYESVIRKERRGEYLGKTVQVIPHITNEIQEFIKRGAAGYEVAIVEIGGTVGDIESLPFLEAARQLSLRSGRNNTAFVHLTLVPFIASAGELKTKPTQHSVQKLREIGISPDALLCRADRRIPDDERAKISLFSNVQEDAVISVWDADTIYKIPQMLHDQGLDRIVCEKLGMSPQPADLSMWDRLVYTLENPKAEVTIGMVGKYVDLTESYKSLTEALRHAGIHTESRVNIEYIDSEELEAKGPGALAKYDAILVPGGFGKRGVEGKIAAARFAREHKIPYLGICLGMQVALIEYARHKAGLTVANSTEFDPDTEQPVVALITEWENHDGKVQTRDASSDLGGTMRLGAQTCDVKPGTLAAEIYGPKVTERHRHRYEANNHYLGRVEEAGLIVSARTPTENLCEIMELPREGADGHPFYLGVQFHPEFNSTPRNGHPLFISFIKAALAHKQQAGAANTAQKETA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein should be able to manipulate the activity of enzymes in the CTP from UDP: step 2/2, Pyrimidine metabolism pathway, either by activating or inhibiting their function.2. The L-glutamine binding site should be located in a region of the protein that is accessible to the ligand.3. The Nucleophile, active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.4. The designed protein must possess ATP binding, CTP synthase activity, metal ion binding to support 'de novo' CTP biosynthetic process under certain conditions.5. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates..6. The designed protein have Glutamine amidotransferase, CTP synthase N-terminal domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "KYVFVTGGVVSSLGKGIAAASLGAILESRGLRVTHLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTSAKMKKCNNFTTGQIYESVIKKERRGEYLGKTVQVIPHITDEIKAHIVRGAAGADVAIIEVGGTVGDIESLPFLEAIRQMGLDEGRNNACYIHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDVLLCRADRHIPEDEKRKIALFCNVQREAVIEALDADSIYKIPAMLHGQMLDEIVRRLDLPFRDVDWSVWDDLLRRVHQPSHELSDVYISVIEAIKHAAWAVGRKPVLTWIDSKELENMERSKSAEVLKGFDGLVVPGGFGQRGVEGIIEAIRYARENKIPYLGLCYGMQLASIEFARNVCGITGAHTTEIEPNASQPIIHIMPEQAKKLLKQDYGGTMRLGSWPCKIKKGTHSHEAYGSDLIHERHRHRYEFNNAYREQLEKAGLVIAGTTPDGQLVEIIELADHPFFVASQFHPEFQSSPLKPHPLFYAFTSMAACR",
    "response#2": "LHDAYLSVAEALSHGGIANDARVDIKWVHSEDLEEDGIDLEEIFSGVDGLLIPGGFGDRGIEGKIKAIRYAREKKIPLFGICLGMQCAVIEFARSVCGLQGAHSTEFDPNTPHPVIDLMPEQKDIEKKGGTMRLGHYPCRITPGTRTMEAYGEEMIEERHRHRYEFNNEYREVLEKAGMVISGLSPDGRLVEIIELKDHPWFVGTQFHPEFKSRPNRPHPLFRDFIINAMQYASGRIG",
    "response#3": "KYVVVTGGVLSGLGKGVTASSIGVLLKSAGLRVTAVKIDPYLNSDAGTMSPFEHGEVFVLDDGGEVDLDLGNYERFLDVCLTSENNITTGKVYDNVIQKERRGDYLGKTVQVIPHITNEIQEWIERVAHIPSDGNGKVPDACVIELGGTVGDIESAPFIEALRQFQFRVGQDNVCFVHVSLIPVMGPVGEQKTKPTQHTVKDLRGLGINPHMLVCRSKSPLIDETRQKVSAFCHVPPEAVISAHDVSNIYQVPIMLDSQGVAGMLSSWFRLPVREPRLEKWRRIVDKIENPSGEVTIGLIGKYVELQDSYKSILESFIHAGAENEVKVKIESIHSEHITPDVIKNKIAKLDGVLVAPGFGERGIEGKIKAVQYAREHKIPFLGICLGMQMAVIEYGRNVCGLEKANSTEMDANTPHPVIGLMDEQKNITEMGGTMRLGAWACKLEPNSIVSDVYGEEVIMERHRHRYEFNDAYREQLEKAGMKITGTNPDTGLVEIIEIEDHPWFVGVQYHPEYKSTVSNPHPLFVAFVEAALKHKNG"
  },
  {
    "reference": "KTLGVNMVRRGVRSLSSKIKQKTKQIGSRPGPSRGVQGFVFFLLFNVLTGKKITAHLKKLWRMLDPRQGLVVLRKVKRVVASLMRGLSSRKRRSQDVLTIQXLILGMLLMVGGVTXVRRNRWLLLNVTSEDLGKTFSVGTGNCTTNILEAKYWCPDSMEYNCPNLSPREEPDDIDCWCYGVENVRVAYGKCDSAGRSRRSRRAIDLPTHENHGLKTRQEKWMTGRMGERQLQKIERWLVRNPFFAATALAIAYLVGSNMTQRVVIALLVLAVGPAYSAHCIGITDRXXIEGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAKFTCAKSMSLFEVDQTKIQYVIRAQLHVGAKQENWNTDIKTLKFDALSGSQEAEFTGYGRATLECQVQTAVDFSNSYIAEMEKESWIVDKQWAQDLTLPWQSGSGGVWREMHHLVEFEPPHAATIKVLALGNQEGSLKTALTGAMRVTKDTNNSKLYKLHGGHVACRVKLSALTLKGTSYKMCTDKMSFVKNPTDTGHGTAVMQVKVPKGAPCRIPVMVADDLTAAVNKGILVTVNPIASTNDDEVLIEVNPPFGDSYIIVGTGDSRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGVIMMFLSLGV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Serine protease subunit NS2B: Required cofactor for the serine protease function of NS3.2. The protein should contain a Helical membrane-spanning region.3. The designed protein should have serine-type endopeptidase activity, ATP binding, double-stranded RNA binding within virion membrane, viral capsid, membrane, host cell mitochondrion to regulate virion attachment to host cell, proteolysis, fusion of virus membrane with host endosome membrane.4. The designed protein have Helicase C-terminal, Helicase ATP-binding, MRNA cap 0-1 NS5-type MT domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "GKPGATRVVNMLKRGASRGNPISRIKRMLDGLLRGVGPVRIVLAILTFFKFTALKPTAGLLRRWKLVGVVEATRHLKSFRKDIGQMLDGLNRRGKKKRGGTTVGIAFLVALGVAQLFSNYFFN",
    "response#2": "RCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGMSYSMCTGKFKIVKEIAETQHGTIVIRVQYEGDGSPCKIPFEITDLEKRHVLGRLITVNPIVTEKDSPVNIEAEPPFGDSYIIIGVEPGQLKLNWFKFKIVKEIAETQHGTIVIRVQYEGDGSPCKIPFEITDLEKRHVLGRLITVNPIVTEKDSPVNIEAEPPFGDSYIIIGVEPGQLKLNWFKR",
    "response#3": "VAXXPHVGMGLETRTETWMSXEGAWKHVQRIETWILRHPGFTIMAAILAYTIGTTHFQRALIFILLTAVAPSV"
  },
  {
    "reference": "MSNTANKVNLLDFSHATLKDYFISIGEKPFRATQVIKWLHQMTAGSVDQMTNLSKSLRTLLEEKAEIRAPEIVIDQQSADGTHKWLLRLADGNAIETVFIPEDDRGTLCISTQVGCALDCTFCSTARQGFNRNLTTAEIIGQLWVAKRTLQADPKAPRVVSNVVLMGMGEPLLNFDNTVNALSLMLDDNAYGLSKRRVTVSTSGVLPALERLGKTIDVSLAVSLHAPNDELRDQLVPLNRKYPIKELLATCRQFLANKTTERNHITWEYVMLDGVNDRDEHAHQLAQLLRGIPSKVNLIPFNPFPSTRYARSSNNRIHRFRDILTEAGYTVITRKTRGDDIDAACGQLAGQVNDKSRRDVHFARIERNN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must have high specificity towards rRNA binding.2. The [4Fe-4S] cluster binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "QHPAFRSRQIYAALYRQRVEDFSGMTVLPAALRAELARTCTVGLPEVSAVFESADGTRRYLLRLADGRSVEAVFMPEESRDTLCISSQVGCPVDCKFCLTAKMGLERNLTAGEIVGQVLLLVRRHHLDPGSRQMNVVMMGQGEPLLNLPNVIAATRILADKEGVGISERRITVSTAGIVPRIAELGRESVRPKLAISLNASSEEQRRELMPITRKYPLRDLLEACRSYPLRSWERLTFEYVLLGGVNDTDQDARRLVRLVASLPCKVNLIALNPGPGIPFQTPDEARVAAFQAIVARSVPCFVRKPRGRDIFAACGQLKSL",
    "response#2": "VFVVSISHQSRASLAMAYNVTIEIMDKLLINLFIKQMGFTYIIGTSLGRKLSLINEAKEVINRKTEEGPILSSICPGWVLYAEKTHPYIIPKLSTIKSPQQITGCLLKNLTCEALQIDKSEIYHLSIMPCFDKKLESARPEIYDNENEEDKSISISVPDVDCVITAKELITLLEECPQYQLIPPQPTQPTLGGDLDLSVTEIYKQYAPPNWPFIEYSWSNDSGSSSGGYAYNYLNIYRNDLVLRKGYDPNKFTINLINGRNSDIYEMRLIYDSKETLASAAVVNGFRNIQNLVRKLKPNTNKSMNNKINPLVARRRARMINRGKSESISQEIEIEIADASKVDYVEIMACPNGCINGS",
    "response#3": "QHPAFRSRQIYAALYRQRVEDFSGMTVLPAALRAELARTCTVGLPEVSAVFESADGTRRYLLRLADGRSVEAVFMPEESRDTLCISSQVGCPVDCKFCLTAKMGLERNLTAGEIVGQVLLLVRRHHLDPGSRQMNVVMMGQGEPLLNLPNVIAATRILADKEGVGISERRITVSTAGIVPRIAELGRESVRPKLAISLNASSEEQRRELMPITRKYPLRDLLEACRSYPLRSWERLTFEYVLLGGVNDTDQDARRLVRLVASLPCKVNLIALNPGPGIPFQTPDEARVAAFQAIVARSVPCFVRKPRGRDIFAACGQLKSL"
  },
  {
    "reference": "MKFRPLHDRVVVRRIESEEKTKGGIIIPDTAKEKPQEGEVVAVGPGARDEQGRVNALDVKAGDRVLFGKWSGTEVKIDGQDLLIMKESDIMGVVAL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein localizes to the cytoplasm. The designed protein sequence is ",
    "response#1": "RRRRRGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS",
    "response#2": "RRRRRGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCR",
    "response#3": "RRRRRGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS"
  },
  {
    "reference": "MLSAHRAEDVRRAEEARMAELEVLGRDPGELMQTAAAAVAATALRMLPRRYGARVLVLAGSGNNGGDALFAGARLRARGVEVWVGATSERGLHVGGERACRRAGARFLARDELVPALTAGRLDLVVDGVLGLGSRGGLPDDVAVLADALDGPGAPPVLAVDLPSGLQADASRADRSFRAARTVTFGTSRIAHLTSPARERCGPVEVVDLGLTLPAPALRQWQAADVAAAYPVPGPTDHKYTRGVVGLDTGSEQYPGAALLGVLGAVHGGAGMVRYLGSATVTTEVPNVVAADGRVQALVLGSGWGSGQDAEDRLRQAFDRVAGEGSALLLDADALAVLAAMLDGGGERPAPDRWLLTPHAGELTRLLQRDVDREWVESHPDEAAQEAADRYRATVLLKGGTQYVAHPRSDDGTDAPPVDLAVPGPAWTGQAGSGDVLAGLCGALLAAGLPAGDAAVVGASLQALAAARRPGPHPPQHVVGAVADVVAELTR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..2. The designed protein should be able to compete with endogenous proteins or inhibitors for K(+), AMP binding.3. A protein with NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, metal ion binding, ATP binding functions is required to enable nicotinamide nucleotide metabolic process.4. The protein must bind to Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MQRLEMAAVTAGITLAQLMENAGAALAQEVESRCRPIQGKRAVLLCGKGNNGGDGFVCAGLLAQRGMTCTVVLTQGEPKTDLAGDAFSRMPDQVQVISGAEEREQAEAAMTAADVIVDCVFGFSFRGELSGVPAHFLGYAGNLSCLKISADLPSGVECDSGRVSAGAFRADVTVTFTGKKPANVSYPAKEFCGETVVRQVGVPAALVADTHKGSYGHTLIAAGTKRMSGAGLLCAKAALRAGCGLATWAVPASLATHLLGRVPEAMLAGLADAGHGDWTHVNAEAILALAEDCDALALGPGMGRYEGDSQWLRGLWEGAGCPLVLDADALNMLAEAPDFADWQPRRAATVLTPHPGEMARLAGVSTREVQRDRIGLARRYAEQHGVTLVLKGARTVVATPDGAVYVNVNGNAGMATAGAGDVLAGIIASLLSQGFDAAQAACLGVYMHGAAGDRAALKRTSPASLLAGDLLEEG",
    "response#2": "MRALDVNAEYFGISRLQLMENAGGSVAAEIALRFKPKETRVAVFCGLGGNGGDGFVAARHLACLGFNVNVILAGKSSEISVEESRRNWAALKHLRRSVTIYEVHDSSLIPNIEADVIVDALLGIGLKAPPRPPIAQLIRAINDSDAFRVAVDVPSGLDSDTGAVLGEAVKANLTVTFHKAKPGLLKAKEYVGELIVRQIGIPSRVQLEQHMDQLIVAGSSGKTGAAALSANSAVRAGSGLVTLAVPESLNPILEVKTTEAMTVPLADSGGGYLTDSAFPAIAGLLPDKDALAIGPGLGQHPATVTLVRALLKAADLPLVIDADGLNAVAQNANCLLDKKSTHVVLTPHPGEMARLMGATIADVEADRIATARKFAAAFGVHLILKGSRTIIAAPDGSTAINGSGNPGMASGGMGDVLTGIIVSLLGQGYNCHDACRLGVFIHGYAADLVAQDKGEIGMCAGDVQEKLTPTLWKNEIPKLEYITNKYTRGYTLVCSVGNKSIGASKLVAMSALRVGSGIVSIACDSNAFAFYASCLTSIMYRLYDDVINDDRITSIIIGPGCGISEITRQRTMDVLSKQNCVLDADSISVFSDSYEILFSKIKHNVIMTPHEGEFKRIFPFLTGSKIEMAQKAASLSKAVIVLKGPDTIIADPVGNVVVNNAPFSLATAGSGDVLSGIIGGLLSSGMNPFDAACCGVWIHTKCAREYGIGLIADDIILQIPQVLKKR",
    "response#3": "MQELDRQTIQEHGIPGLELMEHAGHACMEAIFHEFGYTGGKRAVIFAGRGNNGGDGYVIARLLLQSGWQVRVCILAERERIGGDAAINLEQLPPAAVSYCTVPGQLAGLLQEELSQTDVIVDALFGTGLNSEVAGINREAVELINAAGCPILAVDIPSGIHGTSGRVLGCAVRATVTVTFAAAKLGHVLYPGADHTGRLVVADIGIPAELVEQASRPPRLVNGPLGIDVSRERRADTHKGSYGHTLIAAGTKRMSGAGLLCAKAALRAGCGLATWAVPASLATHLLGRVPEAMLAGLADAGHGDWTHVNAEAILALAEDCDALALGPGMGRYEGDSQWLRGLWEGAGCPLVLDADALNMLAEAPDFADWQPRRAATVLTPHPGEMARLAGVSTREVQRDRIGLARRYAEQHGVTLVLKGARTVVATPDGAVYVNVNGNAGMATAGAGDVLAGIIASLLSQGFDAAQAACLGVYMHGAAGDRAALKRTSPASLLAGDLLETAESIALLLRRRDRQAHKGHFGHCLIVAGSSGKTGAAALSANSAVRAGSGLVTLAVPESLNPILEVKTTEAMTVPLADSGGGYLTDSAFPAIAGLLPDKDALAIGPGLGQHPATVTLVRALLKAADLPLVIDADGLNAVAQNANCLLDKKSTHVVLTPHPGEMARLMGATIADVEADRIATARKFAAAFGVHLILKGSRTIIAAPDGSTAINGSGNPGMASGGMGDVLTGIIVSLLGQGYNCHDACRLGVFIHGYAADLVAQDKGEIGMCAGDVQEKLPYAYQKLL"
  },
  {
    "reference": "MTDGTRELVIAMDGPAGAGKSTVARIVANRLGYLYIDTGAMYRALALKALRLGIPETDAAALADLADATEVELQRAPDGGNRVLLDGEDVTAEIRSPAVSAVVSQVSAVPRLRQRLIEVQRSMARAGGVVMDGRDIGSYVLPHADRKFYITASLQERARRRVAQLRAEGHEADLAAVEAEIARRDEQDMNKGVHSLVQLPESIVIDTTGKRVDEVVEEILRHCRRT",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that can perform dCMP kinase activity, CMP kinase activity, ATP binding in cytoplasm to support pyrimidine nucleotide metabolic process, phosphorylation.2. The ATP binding site should be stable and able to withstand changes in environmental conditions.3. The protein should be designed to catalyze the following reaction ATP + dCMP = ADP + dCDP. The designed protein sequence is ",
    "response#1": "IAIDGPAGAGKSTVAKKLAKLLGFTYIDTGAMYRAITLKVLRENISLEDEERIVEVARKSDISLDGERVFLDGEDVSEEIRKPIISQKVSVVSQIPEVREILVKKQRKIAEGKNVVMDGRDIGTVVLPDAQFKFFLTASLEERARRRYEELKNKGTEVKYEEVLEEIKKRDSLDSGRKTSPLTIPEGAILIDTTDLTEEEVVERVYEAIAA",
    "response#2": "VIAVSGQPGSGKTTIAREIARVLGLPLVSSGLLFREMAARMGMDFIEFHKYAETNPDIDKKVDSLAIERAKAGDVVLEGHLTAWIVRPYADVCIYLKASLETRARRVALRDGKSLQDALREVAEREELNRRRYLSIYGIDINDLSIFDLVLDTD",
    "response#3": "VIAVSGQPGSGKTTIAREIARVLGLPLVSSGLLFREMAARMGMDFIEFHKYAETNPDIDKKVDSLAIERAKAGDVVLEGHLTAWIVRPYADVCIYLKASLETRARRVALRDGKSLQDALREVAEREELNRRRYLSIYGIDINDLSIFDLVLDTC"
  },
  {
    "reference": "MENQSLTFVGDEEAKVRKSTKFHPSIWGDYFIQNSSLSHAEESTQRMIKRVEELKVQVKSMFKDTSDILQLMNLIDSIQMLGLDYHFENEIDKALRLINEVDDKSYGLYETSLRFRLLRQHGNHVSTDIFNKFKGDNGSFISSLNGDAKGLLSLYNASYLGTHGETILDEAKSFAKPQLISLLSELEQSLAAQVTLFLELPLHKRVKILLVRKYILIYQEGAMRNNVLLEFAKLNFNLLQSLYQEELKKISIWWYDLALAKSLSFTRDRIVECYYWVLTLYFDPQYSHSRLIYSKVISLVSIMDDIYDNYGTLEECRQLTEAIKRWKPQAIDSLPEYLKYFYLKLLKTFEEIGEELEHNEKYRMLYLQDQIKAIAVAYLEEAKWSIERHVPSLDEHLHYSLITSGCSLVPCASYVGMGEVATKEVFDWHSSFPKAVEACCAIGRILNDITSYEREQGRGDNASTVESYMKDHGTNEKDACKKLQEIVEKAWKDLNQESLNQKNISRLIIERLVNFSRSMEEIYMSNDMYTNSGTKMKGNITLVLVEAFPV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be designed to catalyze the following reaction (2E,6E)-farnesyl diphosphate = (+)-germacrene D + diphosphate.2. The protein design must feature the DDXXD motif motif.3. Ideal protein should have a binding affinity for key enzymes in the terpenoid biosynthesis pathway to enhance the overall efficiency of the pathway.4. For general function, the protein need meet that Involved in the biosynthesis of germacrene D5. The stability of the protein-Mn(2+) complex should be optimized to maximize enzymatic activity.6. The protein must have high specificity towards magnesium ion binding. The designed protein sequence is ",
    "response#1": "SLLRVAEIDSIRLKSLTQGEMSQTFKWWTELGLEKDVEKARSQPLKWHTWSMKILQDPTLTEQRLDLTKPISLVYVIDDIFDVYGELEELTIFTRVVERWDHKGLKTLPKYMRVCFEALDMITTEISMKIYKSHGWNPTYALRQSWASLCKAFLVEAKWFNSGYLPTTEEYMKNGVVSSGVHLVMLHAYILLGEELTKEKVELIESNPGIVSSAATILRLWDDLGSAKDENQDGTDGSYVECYLNEYKGSTVDEARTHVAQKISRAWKRLNRECLNPCPFSRSFSKACLNIARTVPLMYSYDDA",
    "response#2": "SLLRVAEIDSIRLKSLTQGEMSQTFKWWTELGLEKDVEKARSQPLKWHTWSMKILQDPTLTEQRLDLTKPISLVYVIDDIFDVYGELEELTIFTRVVERWDHKGLKTLPKYMRVCFEALDMITTEISMKIYKSHGWNPTYALRQSWASLCKAFLVEAKWFNSGYLPTTEEYMKNGVVSSGVHLVMLHAYILLGEELTKEKVELIESNPGIVSSAATILRLWDDLGSAKDENQDGTDGSYVECYLNEYKGSTVDEARTHVAQKISRAWKRLNRECLNPCPFSRSFSKACLNIARTVPLMYSYDDEI",
    "response#3": "MNPILLELAKVDYNIVQAVHQEDLKYVSRWWKKTGLGEKLNFARDRVVENFFWTVGDIFEPQFGYCRRMSAMVNCLLTSIDDVYDVYGTLDELELFTDAVERWDATTTEQLPYYMKLCFHALYNSVNEMGFIALRDQEVGMIIPYLKKAWADQCKSYLVEAKWYNSGYIPTLQEYMENAWISVTAPVMLLHAYAFTANPITKEALEFLQDSPDIIRISSMIVRLEDDLGTSSDELKRGDVPKSIQCYMHETGVSEDEAREHIRDLIAETWMKMNSARFGNPPYLPDVFIGIAMNLVRMSQCMYLYGDGHGVQENTKDRVLSLFIDPR"
  },
  {
    "reference": "MADIETKSSQNQPLKTQNIFIGAQIFLRIVVIAASFASTWLMLTNKQTIDIGGFVLDANYSYSPEFKFLSYANIVVGAFSFVSLLFLVLVGRRSSNPTYYFILFLHDLALMSLVLGGCAAATVIGSLGKYGNSHTGWMQICDHFGKFCKRATTSVAFSYFSLVCLLILTITSASKSRQIQV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should contain Extracellular topological domains. The designed protein sequence is ",
    "response#1": "NCGDTSNNVDVSGRNWTAENQKILSSNLVNASFTAQASYQESGVSQIPYMTARIFRSEFTYSFPVTPGSNFLRLYFYPTRYGSQFNAVKSFFSVKVNGFTLLNNFSADLTVKASKPQTEFIIKEFIIPVYQTLNLTFTPSLDSLAFVNGIEIVSIPNRFYSKGGFDDVITNVGSSVDFHIENSTAFETVYRLNVGGKTVGDSGMFRRWVSDDEIILSESSGISPIVPDIKINYTEKTPSYVAPDDVYATSRSMGNADHPEQNLNFNLTWLFTVDAGFSYLVRLHFCETLSEVNKEGQRVFSIFIENQTATLEMDVFRMSGGSWIPMYLDYTVIAGSGSGRRHDLRLDLHPLVSINPKYYDAILNGVEILV",
    "response#2": "NCGDTSNNVDVSGRNWTAENQKILSSNLVNASFTAQASYQESGVSQIPYMTARIFRSEFTYSFPVTPGSNFLRLYFYPTRYGSQFNAVKSFFSVKVNGFTLLNNFSADLTVKASKPQTEFIIKEFIIPVYQTLNLTFTPSLDSLAFVNGIEIVSIPNRFYSKGGFDDVITNVGSSVDFHIENSTAFETVYRLNVGGKTVGDSGMFRRWVSDDEIILSESSGISPIVPDIKINYTEKTPSYVAPDDVYATSRSMGNADHPEQNLNFNLTWLFTVDAGFSYLVRLHFCETLSEVNKEGQRVFSIFIENQTATLEMDVFRMSGGSWIPMYLDYTVIAGSGSGRRHDLRLDLHPLVSINPKYYDAILNGVEILN",
    "response#3": "NCGDTSNNVDVSGRNWTAENQKILSSNLVNASFTAQASYQESGVSQIPYMTARIFRSEFTYSFPVTPGSNFLRLYFYPTRYGSQFNAVKSFFSVKVNGFTLLNNFSADLTVKASKPQTEFIIKEFIIPVYQTLNLTFTPSLDSLAFVNGIEIVSIPNRFYSKGGFDDVITNVGSSVDFHIENSTAFETVYRLNVGGKTVGDSGMFRRWVSDDEIILSESSGISPIVPDIKINYTEKTPSYVAPDDVYATSRSMGNADHPEQNLNFNLTWLFTVDAGFSYLVRLHFCETLSEVNKEGQRVFSIFIENQTATLEMDVFRMSGGSWIPMYLDYTVIAGSGSGRRHDLRLDLHPLVSINPKYYDAILNGVEILP"
  },
  {
    "reference": "MKIVFATMNEHKVAELRQMLAGLCAEIGLEIVSMAEFGDLADVVETEVTFTGNAMLKARTIAAATGLPALADDSGLSVKAMGGSPGVFSSRWSGSRAGAALSRAERDRANIALLLDQLDEVADKYRDASFVCAAVFALPNGLVRTAEGQMHGRLAREPRGTNGFGYDPIFIPVDQPAGEERTLGEFTDAQKNAISHRGRAMRRMAPHIRELLTQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be able to modulate nucleotide metabolic process in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "LDCQWISKCFEKYPSDAHKGTFGRVFIIAGSVGMTGAVALSGLASVRGGAGLVTVGIPESLNDILEVKLTEAMTLPLAESTNRCISNAALNAALEFASKCNVVVLGPGISTNEETKQFVLEFVQNCEVPMVVDADGLNILAEYPHILANAKAKVVITPHAGEMAKLLASTPSKIQEDRLKAAKTAAQRYNCVVVLKGARTLIANPEGMLCINPTGNAGMASGGSGDVLSGIIGAFLARGMQPYNAAAAGVYIHGLAGDIAAQNKGKISLAAGDIIEYLHEAIRKA",
    "response#2": "IADHKYARGHLTILAGPHATGAARLAALAGRRVGAGLVTIAAFRSTLPAFQAAEPGNLIVEADDGTMFAHLLGDPRRNAVLIGPGSGTDGRTRESVLSALEARRSVVLDADAITVFAADPDLLFRSVRGPTLLTPHEGEFRRLFPDLDGVVGKLERVRRAAARSGATILLKGPDTVIAEPGGRAIINVHASPALATAGSGDVLAGTIGGLLAQNLTPFAAASAAAWLHGECALTFGGSGLIAEDLPALLPRL",
    "response#3": "LVVSGPAEATGAARLGARGALRAGAGLVTVASPLDAVGVNAMHLTAIMLRPFEAPEGLASLLEDTRKNAVLLGPGAGLGEATRRLVEAALRSPAAVVLDADALTSFAGDLSRLKDAMALRRAPVVLTPHDGEFERLFGHVPGSRLVRARHAADESGAIVLLKGADTVIAEPGGRAAITANAPPWLATAGAGDVLGGFVTGLLAQGMSAFEATAAAAWLHGEAARDR"
  },
  {
    "reference": "MEIRRLGRVDFEPAWRAMQDFTAGRTPETPDELWVVEHPPVFTLGQAGKREHILNDVGIPVVKIDRGGQVTYHGPGQVVIYLLLDLQRLKIKVRELVNAIEQAIIDFLAEHGVAAGRRPGAPGVYVGDAKIAALGLRIRNGCSYHGLSLNVAMDLSPFAAINPCGYVGLKVTQTSELGIXCSPRPRPANTFAGICGDNWTNDMAEQGVKQKGELKTARIPIKIVPVDQPLKKPEWIRVKAGNSAGRFGEIKSMLREKKLHTVCEEATCPNIGECFGRGTATFMILGDICTRRCPFCDVGHGQPLPPNPDEPRELAESVAALKLQYVVITSVDRDDLRDGGARHFVDVVRAVREASPATTIETLVPDFRGRLDIALDILGEGLPDVLNHNLETVPRLYKQARPGADYAHSLQLMKDFKARYPQVSTKSGLMVGLGETDEEILDVMRDLRAHYVEMLTIGQYLAPSGHHLPVSRYVHPDVFRMFEAEAKKMGFTGAACAPMVRSSYWADQQAHSAGVA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must bind to [4Fe-4S] cluster in order to perform its enzymatic function.2. The protein must exhibit the following characteristics: Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.; Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate- dependent enzymes.3. The designed protein should interact with enzymes and substrates within the Protein modification, protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 2/2 pathway to enhance or regulate its activity.4. The designed protein must have a Acyl-thioester intermediate active site that is highly conserved among related enzymes.5. The protein contains novel Radical SAM core, BPL/LPL catalytic domains that confer a unique function or activity.6. The ideal protein would be one that plays a critical role in protein lipoylation through lipoyl(octanoyl) transferase activity, 4 iron, 4 sulfur cluster binding, lipoate synthase activity within cytoplasm. The designed protein sequence is ",
    "response#1": "MILGDVCTRACGFCNVTTGRPQGLDLDEPVRVAKAIAAMGLSHAVITSVNRDDLPDGGSGIFAQTVREVRRRLPECDVEVLIPDFKGVRADLERVMAEGPAVLNHNTETVLRLQRDVRTSARYGRSLALLARAKWYRPEGVVKSGLIVGMGETRAEVLGALQDANALQHGLYDTAVDDHLLLSEHKPVYTLGVRADEGNVLVDPASVGAELVRTDRGGDVTYHGPGQLVGYPILHLPGKGGGTKPDSAAYVHDVEQLVIDSLADLGLAADRLPDYPGVWVGVGTDAPRKVCAIGVRLNRGRTLHGFGLNVDPDLTMFNHIVPCGIPDKAVTSLRAEGIDATMAEVVDVVVREALDLQRRLWAHSADNYLLLLEHHHVFTAGPSADEANLLVDPGEVGADCVRIDRGGDFTYHGPGQLVGYPILTLPGRRGGGLAETAVYVCDLEQVLIDALADLGLPGCGRLRGYPGVWLDLNGTPRKLAAVGVRLSRGRTMHGFALNVDTDLSWFERIIPCGIRDFEPTSLRAEGCAATMREVTDAVRRCFPGRWGAGTATFMILGDTCTRSCGFCAVKTGRPGIVDLEEPKRVGAAVAQMALGHAVVTSVNRDELRDGGASLFAATIREIRRQSPGTTVEVLIPDFTGDPAALDVVLAERPEILNHNVETVERLYPRVRPQARYPRSLGVLRRTTERAPELTSKSGIMVGLGETRDEVVATMRDIAAQGTNVLTIGQYLRPSPTHLPIERYWTPAEFAALRHEGMGMXALGTRRMPPWLHKRLPASGDIEATRRLLADLHLNTVCQSARCPNQGECFASRTATFMILGNTCTRNCRFCAVEHGQPEAIDEDEPRRVAEAARRLGLKHVVVTSVTRDDLPDGGAGHFAATITALRQALPGAYIEVLTPDFRGSRAALAAVARARPDIFNHNVETVPRLYPDVRPQADYRRSLDVLKQMKELDATIYTKSGLMVGVGESREEVLAVMADLRAVNCDILTIGQYLRPSPRHLEIKEYVPPETFTWYAQKGREMGFLYVAAGPYVRSSYHAAEFS",
    "response#2": "MILGDICTRRCPFCDVAHGRPLPPDPEEAEKLGKTIRDMKVKYVVITSVDRDDLRDGGAQHFADCIREIREHSENNIQVEVLVPDFRGRMQVAIDILKGEAPDVFNHNLETVPRLYKAARPGANYQWSLDLLQKYKEVRPDIRTKSGLMVGLGETKEEILEVMKDGEDAIMPWNDGTATFMINGERCTRACGFCLVDTRKPEPLDLDEPRRVAEAVEKLGLEYAVITTVARDDLADDGASGFTRTVAAIRDRVPQCKVELLISDCRGRASSLDEIFATRPEVLNHNIESVARLQRAVRPSAGYARSLAVLARASAAGLTTKSSIIVGMGETFDEVVGTLADLAGIGVNIVTIGQYLRPTTNHLPIHRWWTPDEFAELKRLGEAMGIEYPAINVNDSVTKSKFDNLDFGRGTATFMILGDLCTRRCPFCDVAHGRPHAPDPDEPMHLANSIAVLKLNYVVITSVDRDDLRDGGAQHFADCIRAIRTQSPQTRIEILVPDFRGRLEVALEKLSACPPDVMNHNLETVPRLYKQCRPGADYMHSLQLLKDFKAAFPHIPTKSGLMLGLGETDEEIIEVMRDLRAHQVDMLTVGQYLQPSKGHHPVMRYVSPEDFKTFERIATNLGFKKKIN",
    "response#3": "WGGGTATFMLLGDVCTRHCRFCAVASGRPGAPDPLEPYRVARAAARLGLRHVVVTSVARDDLPDGGAGQFAATIRAVRERLPHATVEVLIPDFGGSVRALELVVAARPDVLNHNVETVPRLYPVIQPRKDYRRALGVLAYAHRAGLKTKSGVILGMGETRGEVIGVLEDLRRFGCHIVTLGQYLQPTDRHLEVAEYIHPVEFEWYREVALAMGFDRWAHLLQRRLVAARQADRVGDVLLVVEHPPVITLGRGGGTEDLRVGADRLAALGVGLELTERGGRATYHGPGQLVFYPIFKLSPGRLHDFLWALEEAAIRTLARYGIAAGRDPEHPGVWVGREKVAAVGLAVSRDVTFHGLALNVAPDLGHFGLIVPCGLADRGVTSMARILGRAPGMLGVERAFCEEVRPVATPAARWAHLLQRRLVAARQADRVGDVLLVVEHPPVITLGRGGGTEDLRVGADRLAALGVGLELTERGGRATYHGPGQLVFYPIFKLSPGRLHDFLWALEEAAIRTLARYGIAAGRDPEHPGVWVGREKVAAVGLAVSRDVTFHGLALNVAPDLGHFGLIVPCGLADRGVTSMARILGRAPGMLGVERAFCEEVRPVATPAAPSEAPWLVAPAPTGAAVARLEGLFRRERLHTVCEEALCPNIGECWGGGTATFMLLGDVCTRHCRFCAVASGRPGAPDPLEPYRVARAAARLGLRHVVVTSVARDDLPDGGAGQFAATIRAVRERLPHATVEVLIPDFGGSVRALELVVAARPDVLNHNVETVPRLYPVIQPRKDYRRALGVLAYAHRAGLKTKSGVILGMGETRGEVIGVLEDLRRFGCHIVTLGQYLQPTDRHLEVAEYIHPVEFEWYREVALAMGFEEAAAGPLVRSSYRAGELL"
  },
  {
    "reference": "MIGSNTAVTLSSAKNHLAKVACNVHKFGGSSLATSACVERVVDIIRQHCQLNDIIVVSANGNTTDALFTLYQLAVELQEITSLNTEQVSTSNDLNTLREHLRVTITALDATQAQLIDELLNASSAARLKSLLKQDVAQITEHLLSDPLSAPLSEPLSYQNDLLAFGEVWSARLLSAVLSESVCPSYMLDARDFLLLKDEKNCAIDYSVSQENFLPLQQQDQLVIVTGYIARNQQQQACTLGRNGSDYSATIMAALSGAGNVTLWTDVDGIYSADPRLVPLARKLHRLPNAVANELGRLGNPVLHAKTLQPLTNHNTHLHVASSFAPEVSGTEIGKFGQIAKQELSVTSLNDLILAKSSSFLAKSGELAVSEFSPICYNLDEGYLVVTQAQQKALSQWLASHDTEVTFRPVAIIAIVGHSVAKQGDMRARFKRALKHQQCLHFLHAFNEHSYIAVLPEPCSSEILNNVHADMTKDAKNIALVVAGLGNVGQRFLTMLPKQIARVAGLDNVHLVALVGASKALINTDGIDVANALLHYQQQAQPYDSEQLLTWLTNHPYDELIVVDITPSEDFSQLYEEFFARGIHVIGANKWAASSSTENYQQLLKTAEKNNSLWLGNTTVGAGLPINSSIKELMNSGDKITEISGIFSGTLSWLFQFYDGTTPFSALLSNALTQGLTEPDPREDLSGRDVQRKLLILARAAGFELSLADIDCQNLVPESLRGISLEAFLQRTSELDELFLLRLQQAQAKACGIRYVARFISDHGQVSA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should be able to enhance the efficiency of lysine biosynthetic process via diaminopimelate. The designed protein sequence is ",
    "response#1": "NPIHQAAGIERIVVSTYQAVSGAGAAGIDELTEAVAAYQGGSNIEPIIFQHPIAFNVIPHIDVFEANDYTREEMKMVFETRKIMHADEMRIAATAVRVPVYRSHSEAISIETRIPITPEEARNILQNAPGIVVLDQPGENKYPMPLFTADSDDVFVGRIRKDISVDNGIVLWVAADQVRA",
    "response#2": "LWPLHKACEIKRVVVTTFQSVSGTGTPAIEELRRQSEQVLEKGIEESCRHGLAKDAYPHQIAFNCLPHIDKFEEDGYTKEEHKMINETRKIFHEPKFRLTATTVRVPVFRGHSEAVNIEFKKAISPEEASAILKYAPGVTLIDDVSKNEYPMPIFCEWKDDVFVGRIRADKSVRYGLDMWVVADNILI",
    "response#3": "LWPLHKACEIKRVVVTTFQSVSGTGTPAIEELRRQSEQVLEKGIEESCRHGLAKDAYPHQIAFNCLPHIDKFEEDGYTKEEHKMINETRKIFHEPKFRLTATTVRVPVFRGHSEAVNIEFKKAISPEEASAILKYAPGVTLIDDVSKNEYPMPIFCEWKDDVFVGRIRADKSVRYGLDMWVVADNILY"
  },
  {
    "reference": "MSDETDRPPKRTSNEPGFGPFGGGPFGVSNRRPPDGSPGPSQPPRGPGSPPGGPWRGWLLPLALFLVYLAWNYSNTAGSAEPSIPYTQFFEQVEQGTIARVQITGQNVTGEFSEPRSVNGRELRRFSTTLPPIQDDALLPLLRERNVEVTVRSDEPSFALYTLLNVFVPFLLIIGFLVWASRRTSKMMGGGPFAGMIKGRAKRFEKESQVSVSFADVAGHKAAKRDLKEVVDFLKEPERFRRLGGKVPRGILLVGPPGTGKTLLARAVAGEAGVPFFSINGSEFIELFVGVGASRVRELFEEAKKVAPAIVFIDEIDAVGRSRGAGLGGGHDEREQTLNQLLSEMDGFTRNDLTIVLAATNRPDVLDPALLRPGRFDRRVVADRPEFEARRAILQVHTKDKPLGPDVDLESVARNTPGFSGADLANLCNEAALSATRRGADHLEARDFSAAFDKIVLGDPRETKLDPEEKRRVAIHESGHAVVAHFSPYAEPLHRVTILPRGMALGITQQTPQAERHVLTRPELEARLRVLMGGYAAEAVVLGDTSSGAENDLKQATDLAFKMVAHFGMSEKIGPVFHEHRSEHPFLGQKMATETATSDATVHIIEEEVRRVLTEAAEDAKRIINEKRTYVDALRDALLEQETLEKAELGVILGPSVKPSPRGETDALHYDSGD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that contributes to protein catabolic process, proteolysis by functioning in plasma membrane with ATP-dependent peptidase activity, zinc ion binding, ATP binding, metalloendopeptidase activity, ATP hydrolysis activity.2. The Zn(2+), ATP binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein must have a active site that is structurally stable and resistant to denaturation.4. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.5. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins; Plays a role in the quality control of integral membrane proteins.6. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "IMSDEEKSITAYHEGGHALVGRLLPGTDTLHKVTIIPRGRALGVTQYLPEDRHSYSRSYLENRLAMLLGGRAAEELVMGEITTGAGNDIENATKMARKMVCNYGMSDALGPLSYGEKQDEVFIGRDLGHYRDYSEDTARLIDAEIKRIVADAHRKAYDLLEENIETLHTIAKALLERETISGEDLNKFSGLFRQ",
    "response#2": "MVMSEEEKLKTAYHESGHAIVGRSLEEHDPVYKVTIIPRGRALGVTMFLPEEDKYSYSHQSILDRICGLYGGRIAEELIYGEKGVTTGASNDIERATELAHNMVTKWGLSEKLGPIKYGDETDEPFLGRSAGASKQGISDDTAHEIDNEVKTILSNCYQHAKDILSENIEALHAMSKALMDYETLDVDQIDDQRQAAIAAAAPKGVLLIGSPGTGKTLLARAVAGEAGVPFFSMSAAEFVEMIVGVGAARVRDLFKQAREHAPAIIFIDELDSIGRARGQTVIGGSSEQEQTLNQILTEMDGFSSREGIIVLAATNQPDVLDKALLRPGRFDRRVVVNLPGFEERLELFRHYLQPRLMTVSAQKIAAGTPGMAGADLENLVNEAALLAARKNHDYVTTEDFEGAIEYIITGRKTGTLGNMLLDDV",
    "response#3": "MVMSEEEKLKTAYHESGHAIVGRSLEEHDPVYKVTIIPRGRALGVTMFLPEEDKYSYSHQSILDRICGLYGGRIAEELIYGEKGVTTGASNDIERATELAHNMVTKWGLSEKLGPIKYGDETDEPFLGRSAGASKQGISDDTAHEIDNEVKTILSNCYQHAKDILSENIEALHAMSKALMDYETLDVDQIDDRLKASRS"
  },
  {
    "reference": "MANLTPMMQQYFDIKKQYPDSLLFFRMGDFYELFFQDAETASRELEITLTGRSCGLEERAPMCGVPHHAATGYIDRLVSNGYKVAICEQIEDVSQAKGIVKRDVVRVITPGTLIDTQLLDDKKNNYLMSVFGSRTGFGLAYVDISTGDLFATEIKENIHPQMLIDEMGRVLPQELLYFIETDKEDPTIISMIKKRFDFYTNGYEEWSYEDTFALNQIKDHFNVVSLEGLGFHPSHLGINAAGALFHYLKTTQKRALEHINHINVYSIHEKMTLDINTRKNLELTETIRSKSKKGSLLGVLDKTSTAMGGRMLRKWIEAPLIDPVIINKRLEAVQLLKEQIELRQELKESLKKIYDLERLAGKISYGSVTPRDLIALKNSLSYLPSIINGLEKIQGETFQSLVQSIDPLDEVHSLVELSILEDAPLSSKDGGIIQEGYHKEVDELKNASTEGRQWIAQLEQKERVNSGIKSLKIKYNKIFGYYIEITKSNLSMVPTEYIRKQTLANCERYVTPELKEIESKILGAEEKVILLEYHLFIEVREKIAHEITRIQQTARAIAELDVLYSFAEIAAENNFIKPHINTSNEIKIVEGRHPVVELTFNKESFVPNDTYMDNRDCSMSIITGPNMAGKSTYMRQVALIVLMAQIGSFVPASEASIGIVDRIFTRIGASDDLAQGHSTFMVEMSEMANILNNATANSLVILDEIGRGTSTFDGLSIAWAVIEYMQQYKKSKTLFSTHYHELTELEGKIQGVKNYNILVEEDGEEIVF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that This protein has a weak ATPase activity.2. The protein must have high specificity towards ATP-dependent DNA damage sensor activity, mismatched DNA binding. The designed protein sequence is ",
    "response#1": "KGRIIEIYGHESSGKTTLTLLAIAQCQKQGGVAAFIDAEHALDPKYAKLLGVDVDNLIVSQPDTGEQALEIADMLVRSGGIDIVVIDSVAALTPKAEIEGDMGDSHMGLQARLMSQALRKLTANIKRSNTLVMFINQIRMKIGVMFGNPETTTGGNALKFYASVRLEVRKGV",
    "response#2": "GYDPDLDALRRAHADPVAYFLDLEVREKESTGIPTLKVGYNAVFGYYLEVTRPYYEKVPQEYRPVQTLKDRQRYTLPEMKERERELYRLI",
    "response#3": "EDNHKIPTNSGIDNLLDGGVEKGTVTQIFGPPGSGKSNISLVLAVNVAKQGKKVVYVDTEGGISINRIKQIAGEDFPKIVNNIIVFEPTSFLEQNENLKTIELWIRKHHDDVDLCVLDSAVALYRVDDMKSSRLNKELGKQMGILAKIARNYDVAVVLTNQL"
  },
  {
    "reference": "MENRWQVMIVWQVDRMRIRTWKSLVKHHMYVSKKAREWSYRHHYQSANPRVSSEVHIPLGEAKLVIATYWGLHTGERDWHLGQGVSIEWRMNRYSTQIDPNXADQLIHLYYFDCFSESAIRKAILGHIVSPRCEYQAGHSKVGSLQYLALAALVKTKQTKPPLPSVAKLTEDRWNKPQKTKGHRGSHTMNGH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be optimized for stability and expression in host cell plasma membrane, plasma membrane where it will participate in viral life cycle.2. The protein must exhibit the following characteristics: Seems to play a role in viral morphology by affecting the stability of the viral nucleoprotein core; In turn, they are directed to the 26S proteasome for degradation. The designed protein sequence is ",
    "response#1": "NRPTHELIAVCSFHNRVTLKSFNIPVFIRALYIRQQGLDSVEQAISSDVDHAITTARVAPYAGLTLVINITSTKGAFKLLKAGSQILAELGPYLTQVSLHDVIMNWKHTGTSYILC",
    "response#2": "MGAASITLTVQARQLLSGIVQQQRNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLSQIWDNMTWMEWEREIDNYTKEIYTLIEQSQNQQEKNELELLELDKWASLWNWFDITQWLWYIKIFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPISLM",
    "response#3": "LSKWIEVPEAVRVSYKNSYHFSLKDMTMTFSSGKQPFNISRLHLAECVPTIATEAIDGIFARKYSSTHVRSGDIEYYLGSGGFLIAFQKLMSHGLAEK"
  },
  {
    "reference": "MKATGTFFFVVGPSGAGKDSLIDGARAALDGDYVFARRVITRPDGSAGEEHEGVTEAEFARRQRSGEFLVTWDAHDLRYGLPKSLMCELERGRNVVANGSRGVIAELAARLPRFVVVLVTAPHDVLARRIAARGRESGDQVASRVARAGAPVPPHVPCITVSNHSTLEAGTARFVEALRTGTRTSAAERPASRTNLMAKLRGEPLDEAAYVAVLQDAIAGRYTEAELTEFLVAATRTLTDEEVVALARARTAFTPRIDWDEPVVVDKHSMGGVPGSRITLIVVPIVAAYGLAMPKTSSRAITSAAGTADAMETIARVDLAHEDVRRCVAQARACIAWNGRLNHSVVDDVMNAITRPLRLDSRRWSVASILSKKYTAGATHVIVDLPYGPQTKLATRADAEALGALFEHVGKGLGLHVRALVTDGSGPIGRGIGPALEVRDVRLVLDNAPDAPADLRDKALRFAGEIIAFDPRVDSPEHGMQIAAALLHEGKARAAFDRIAAAQGVRCDPVAPGTHTLVVPATTRGRVAGVDGLQISGVARAAGAPRDGGAGVDMLCAIGAKVAPGQPLYRIHSDSAEALEAAAALVRAGGECCQAVRIDPD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Ideal protein should have a binding affinity for key enzymes in the Metabolic intermediate biosynthesis, 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis pathway to enhance the overall efficiency of the pathway.2. The protein design must be able to perform ribose 1,5-bisphosphate phosphokinase activity, ATP binding in order to contribute to organic phosphonate transport, pyrimidine nucleobase metabolic process.3. The protein have enhanced phosphate + thymidine = 2-deoxy-alpha-D-ribose 1-phosphate +   thymine.4. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of ribose 1,5-bisphosphate to 5-phospho-D-ribosyl alpha-1-diphosphate (PRPP).. The designed protein sequence is ",
    "response#1": "MKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGADRDLHASQIQGFFNIPVDLHAGQIQGFFDIPVDHLYAPILAKYMQEKFGNADDIVVVSPDVGSVSRSRKFAEKLDAPLAIIDKRRPKANVCEIMNIIGDVRGKRAILVDDIIDTAGTIVSAANALADIGAKEIYACCTHGVLSGPAIERIKNSPIKELVALNAAPTLASYFRRKKLENVVVVSPDHGGVTRARVFASFFNAPLAIIDKRRPEPNKAEVMNIIGDVKGATCIMIDDIIDTGGTLMAGANALKEAGAKEVYAAATHGVLTSNATERLQNSVINEIVITDTIPVTKAQAEPVIIDYLRQKNIDNLTVVAPDVGNIKTASRYATALGGELAIIHKRRLTGKEVLFEEIIGNVKDRNIVMVDDMISTAGTICGAAHMVKKLGARRILAGATHGVFSSPAVERFQSAPIDEVVVTDTIPLTTEQEFADKIRLIAPILAKYMQEKFGNADDIVVVSPDVGSVSRSRKFAEKLDAPLAIIDKRRPKANVCEIMNIIGDVRGKRAILVDDIIDTAGTIVSAANALADIGAKEIYACCTHGVLSGPAIERIKNSPIKELVALNTIPVTKAQEFGDKIRIIVFSGTANPDLTQAICSYLNVKPGQRTIERFPDGEKMIKLDSEVRGRDCFVVQPTCAPVDENLMELLIFLDCLRRASADRVTAVVPYYGYARQDRKDAGRVPITAKLIANIITTA",
    "response#2": "MKVFSGNANLPLANAICDYIGIKLGKCTVKPFPDGETFVKIDENVRGEEVFVVQPTSPPTNHNLMELFIMMDALRRASAARITAVLPLYGYARQDRKDQPRVPITAKLVANLLVAAGVDRVLTMDLHASQIQGFFNIPVDHAAPVMYEYLRKKKLGDLVVVSPDVGGLKMAYAYSQTLDASLAIVAKRRKSATEVESMGVIGEVRGKNVLLVDDLTETAGTLTQAAELLKKKGAKQIIACVSHAILSTTGIERLRKSKIDELITTDTIPLSAEFADKVTVVSVAPLLAEAMRRISHEQSISELFV",
    "response#3": "MKVFSGNANLPLANAICDYIGIKLGKCTVKPFPDGETFVKIDENVRGEEVFVVQPTSPPTNHNLMELFIMMDALRRASAARITAVLPLYGYARQDRKDQPRVPITAKLVANLLVAAGVTRVITLDLHASQIQGFFNIPVDELTAAPVMYEYLRKKKLGDLVVVSPDVGGLKMAYAYSQTLDASLAIVAKRRKSATEVESMGVIGEVRGKNVLLVDDLTETAGTLTQAAELLKKKGAKQIIACVSHAILSTTGIERLRKSKIDELITTDTIPLPPDHPLDKITQISVAPLLARAIREVFDDGSVTSLFDGRAK"
  },
  {
    "reference": "MNQSISSLIKLKFLQSHSNDKNGNKKGGSNVSTGIDKLRESESYRSPFLQLAEIQEHTNNDDDKLDVKECEPTKKHSKLSRIRRKMGRLDLNFRSANEKGSEDDEILVAQHLRNGQDPEEMPFKSENNIDSIEKVPKPDGERVTLTSSGSDNVKRNSKHAPFIPVKPALEKFPSSNRLSRDYRKSQEPTLFNGDRLVPTLPTVSRISTSSSVGSSTAASRYFNPSKRAVVASSSSSSSSIKFNSLHAIPLDATPQIELAKQQDEISKRRFGRRRSRTVDVFDYINKNNTAKNKPPLSPSSFIRTIDEKNTNSLMQDPMGSRGPLLPDDANIISNDTDGAEASHPDHQVLSRSRSQSTSFVQGKGGKRKSIEDEGYHNKLGLPHGSGPTSVYNNKSNANSTITGMSRRSSSIVNALSSFVNLRSSSLSSSRQQHLQQQQQLQQKLDVSLEDLPPVPAPEFSDSCKDFLIKLAPYGKFIGIILTEKDDEFKKNCLNYLLTNCFEFKNDPLDIALRKLLMFLELPKETQQIDRLIMAFSFAYYKAQKSYSKKKGIECPWSNADQVYFIAFSLLMLHTDYFNPNNKSKMTKHDFVDLVHNDKYSGGNEIPMAVLTYFYENVTAKESPKFNYFLMSPMALDDSILDKDAFDTNFAITLSSNSMYSPIDMIKRGSIIPKEASLSPIFYPLTNSISASGIAPSTAASCPPSTSGTINGANLGTANSNSNRPASNSISSYFSYNPSSSSSGNATLVQDDINVYSHIINDTLNEVNL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that can perform guanyl-nucleotide exchange factor activity in mitochondrion to support exocytosis, protein localization to Golgi apparatus, response to endoplasmic reticulum stress, vesicle-mediated transport.2. For general function, the protein need meet that Has no exchange activity on Rab GTPases; Involved in vesicular transport.; Catalyzes both the GDP release by and the GTP binding to ARF2; Guanine nucleotide exchange factor for Arf GTPases, stimulating the nucleotide exchange from the GDP-bound to the GTP-bound form3. Target a Polar residues, Basic and acidic residues compositional bias in the protein's composition for improved properties.4. The designed protein have PH, SEC7 domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MRKLVLSGFLASEEIYINQLEALLLPMKPLKATATTSQPVLTIQQIETIFYKIQDIYEIHKEFYDNLCPKVQQWDSQVTMGHLFQKLASQLGVYKAFVDNYKVALETAEKCSQSNNQFQKISEELKVKGPKDSKDSHTSVTMEALLYKPIDRVTRSTLVLHDLLKHTPVDHPDYPLLQDALRISQNFLSSINEE",
    "response#2": "LSTGIPKEWRRKVWLTLADHYLHSIAIDWDKTMRFTFNERSNPDDDSMGIQIVKDLHRTGCSSYCGQEAEQDRVVLKRVLLAYARWNKTVGYCQGFNILAALILEVMEGNEGDALKIMIYLIDKVLPESYFVNNLRALSVDMAVFRDLLRMKLPELSQHLDTLQRTANKESGGGYEPPLTNVFTMQWFLTLFATCLPNQTVLKIWDSVFFEGSEIILRVSLAIWAKLGEQIECCSCLCILSHFPWFEVFYKILNNVGDLLAQNQVAEAEELLQNLQQHPLLGPRFSGRSEMDSSITVRSECGILPPALGNSKLLSCFVAPDAASLPSIPENRNLTELVVAVTDENIVGLFAALLAERRVLLTASKLSTLTACVHASCALLYPMRWEHVLIPTLPPHLLDYCCAPMPYLIGVHGSLAERVREKALEDVVVLNADSAKGIPKEWRRKVWLTLADHYLHSIAIDWDKTMRFTFNERSNPDDDSMGIQIVKDLHRTGCSSYCGQEAEQDRVVLKRVLLAYARWNKTVGYCQGFNILAALILEVMEGNEGDALKIMIYLIDKVLPESYFVNNLRALSVDMAVFRDLLRMKLPELSQHLDTLQRTANKESGGGYEPPLTNVFTMQWFLTLFATCLPNQTVLKIWDSVFFEVSDGIPKQIRGIIWQLISNSKSKEIRQLYQDLLQIPSEHEKAIQRDISRTKFIPVDKTESLFNVLKAYSLFDPEVGYTQGMAFVTAPLLINVWEEADAFGLLIKLMKNYGLREFFLPDMPGLQLKLYEFDRLLEENSPQLYNHLIRLGIRSSMYATQWFLTLFAYKFPLGFVLRILDVIFVEGIESLLKFAVILMLKNESVLVQL",
    "response#3": "VSDGIPKQIRGIIWQLISNSKSKEIRQLYQDLLQIPSEHEKAIQRDISRTKFIPVDKTESLFNVLKAYSLFDPEVGYTQGMAFVTAPLLINVWEEADAFGLLIKLMKNYGLREFFLPDMPGLQLKLYEFDRLLEENSPQLYNHLIRLGIRSSMYATQWFLTLFAYKFPLGFVLRILDVIFVEGIESLLKFAVILMLKNESVLVQLFGFLASEEIYINQLEALLLPMKPLKATATTSQPVLTIQQIETIFYKIQDIYEIHKEFYDNLCPKVQQWDSQVTMGHLFQKLASQLGVYKAFVDNYKVALETAEKCSQSNNQFQKISEELKVKGPKDSKDSHTSVTMEALLYKPIDRVTRSTLVLHDLLKHTPVDHPDYPLLQDALRISQNFLSSIGARSCLCILSHFPWFEVFYKILNNVGDLLAQNQVAEAEELLQNLQQHPLLGPRFSGRSEMDSSITVRSECGILPPALGNSKLLSCFVAPDAASLPSIPENRNLTELVVAVTDENIVGLFAALLAERRVLLTASKLSTLTACVHASCALLYPMRWEHVLIPTLPPHLLDYCCAPMPYLIGVHGSLAERVREKALEDVVVLNADSQ"
  },
  {
    "reference": "MTANIFAVILAAGKSTRMHSRLPKVLHPLLGRPMALYVVDAARAATGTEPVVVVGHAAEQVRQSLGDSAHFVLQDPQLGTGHAVMQVEPLLRGRAETILVVTADMPLLTAETLQALIRVHLANACPITLLTVVSEDSRGFGRIVRDQDGRIREIVEEAHAAADQLALRELNASVYCFAADWLWQALRRIPLSPKGEYYLTDLVGIAVADGLPVQTVESKDLDETIGINTRVQLAQAEAILRQRLNRHWMLAGVTMIDPDSTYIEAGVQIGQDTTLWPNTYLLGSTVIGEACIIGPNTLIRDCRIGNGCRVLASVLEQAVVDDHVEIGPFGHLRKGAHLAEGVHMGNFGEVKNAYLGPGTKMGHFSYIGDATIGAEVNIGAGTITCNFDGKQKHHTEIGDEVFIGSDTMLVAPVKIGSGARTGAGAVVTRDVPEHTLAVGVPARAIRKLKSSD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should take into account the physiological conditions of the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, Nucleotide-sugar biosynthesis pathway.2. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).3. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.4. A protein that can perform UDP-N-acetylglucosamine diphosphorylase activity, glucosamine-1-phosphate N-acetyltransferase activity in cytoplasm to support cell wall organization, cell morphogenesis, regulation of cell shape.5. The designed protein should contain one or more MobA-like NTP transferase domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MTILAAGKGTRMKSQLPKVLHKLGSKSLVERALNSCDLIKPDRSLVIIGYQGEKVKSALNHLSGVEFVEQTEQLGTGHAIQQLMPPLDDFEGDLLVLNGDVPLLRSQTIEELVKTHQENKNDATLLTANMPNPKGYGRVFCDSNNIVTQIVEDRDCNRAQKQNNRINAGVYCFNWQKLKAVLPKLSTNNDQKEYYLTEVFDYLGKDPNELCGEYICDDIIGIMDINDRDDYEQANKILKERINNKFMEEGITLLDPSRFDLRGEIVSHGQDVEIDVNVLIEGQVKLGERVKIGPNVIIKDSHIGDDVEIFANSLIDRANIGAGSHIGPFARIRPESTLGEYVHIGNFVEIKKTTVGNSTKINHLSYIGDSIIGSNVNVGAGTITCNYDGANKHLTIIEDGAFIGSDTQLVAPVKIGRNATIGAGSTITKNAPDGQLTLSRSKQENIEGIKKKMLIK",
    "response#2": "MAAIILAAGEGKRMKSKHSKVSHKICGKPMVQYVVNSVKGAMIDDVAVVVGHRAEEVKGCIKGDVTFVLQEKQAGTGHAVMCCEQFLEGKKGTTLVLYGDTPLISKDTIKDLVAFHNENNFSSTVLTANFKDPAGYGRIVRDTSGNIQRIIEDKDATEKEREIKEINSGIYCFDTEELIDALKQLHNDNSQGEYYLTDTLGIIKRKGLKVGAFLVKDFDESMGVNDRVALSQAEEVMRQRINRKHMLNGVALADPARIDVRGTLRCGHDVQIDVGCVFEGDVELGEGVHIGPYCVIRNSHIGAECRIDAHSVIDGAVLAGLNNVGPFARLRPGSRLAVRARVGNFVETKNAEVGEGSKINHLSYIGDARLGRDVNVGAGTITCNYDGANKHRTDIGDEAFIGSNSALVAPVSIGKAATIGAGSTISRDVADHSLAVGRSRQENKEGYAEKLKKKK",
    "response#3": "VIVLAAGAGTRMKSKTPKILHEIGGRSLVGHALHGAAALNPDHLVAVVSHERERVTAAINDVAAELGRTVVIAEQEQPLGTGDAARAGMTGLPADFDGTVIVTVADAPLLDGETLSALVDEHLRDDRGEAAAVLTAVVPNPDGYGRVIRGADGTVAGIVEQKDATAEQREITEINSGVYVFDAAVLREGLSRLGSDNVQGEEYITDVIGIVRADGGRVGGHLAADWLQVEGVNDRVQLAALGREMNHRLLRRWMLAGVTLSDPVRIDVRGTLACGRDVFIDINAVFEGDVTLGDRVRIGPNVLIRDSELGDDVEVRPCSLIDGAVVAAGCTIGPFARLRPGTQLAESARIGNFVEIKQSDIGACSKVNHLSYIGDTTIGRDVNVGAGTITCNYDGANKHRTEICDGAFIGSGVELIAPVMIGANATIGAGSTISKNTEAGKLTMARCRQRNIEGWVARLRPGTGAAQAAAEALRAREGET"
  },
  {
    "reference": "MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAFDNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQGEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAGTAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVLGLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFAMAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEKMADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQSIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Consists of two halves formed by TMs 1-5 and 6-10; The plug probably moves laterally to allow the channel to open; The ring and the pore may move independently.; The central subunit of the protein translocation channel SecYEG2. The ideal protein would be one that plays a critical role in SRP-dependent cotranslational protein targeting to membrane, translocation through signal sequence binding, protein transmembrane transporter activity within cell envelope Sec protein transport complex, plasma membrane.3. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "FNEDGAKKFADLTARNVGRQIAILLDGKVLTAPRVSEPITGGNAQITGSKDAKEAEHLAILLRSGSLPVKLEVVENRTVGPTLGQDAKDASMKAFAIGLAGVFLFMLLYYRLSGLVADIVLLLYTLLLLAVMKGLNATLTLPGMAGIILSIGMAVDANVLIFERFKEEIANGKTLRAAVNSGFSRAFTTILDSNVTTLMAAAVLFYLGTGPIKGFAVTLALGVLISMFTAMY",
    "response#2": "FNEDGAKKFADLTARNVGRQIAILLDGKVLTAPRVSEPITGGNAQITGSKDAKEAEHLAILLRSGSLPVKLEVVENRTVGPTLGQDAKDASMKAFAIGLAGVFLFMLLYYRLSGLVADIVLLLYTLLLLAVMKGLNATLTLPGMAGIILSIGMAVDANVLIFERFKEEIANGKTLRAAVNSGFSRAFTTILDSNVTTLMAAAVLFYLGTGPIKGFAVTLALGVLISMFTALFSNYFFN",
    "response#3": "VSEFGSKEEILIKFPFVETAENEDLNAIVANILKPSGDFEIRKFDTVGPRVGSELKEKGILSLILALIAIMVYVSFRYEWRFALASVIALVHDVILVASSVIVFKIDMNLEVIAALLTLIGYSINDTIIIFDRIREEMLSQKTKNATQAIDEAISSTLTRTLLTSLTVFFVVLILCVFGSKIIIGFSLPMLIGTIVGTYSSS"
  },
  {
    "reference": "MSNTHFHCRKLKNKLMLTLSFLAVFFGLFWLCWILFTLITKGIPALSLELFLEKTPGPGEKGGLLNAIIGSTLMITVSTLIGTPIGILAGTYLAEYGRYSQLTKVTRFLNDVLLSAPSIVIGLFIYAIYVSQVKHYSGWAGAFALAIIIIPVVVRTTDNMLNLVPNNLREAAASLGCPQWRVITMICYRSARAGILTGVLLSIARISGETAPLLFTALSNQFTSFDMNGPMANIPIVIYQFAASPFQDWNELAWAGATLITLFVLLLNIFARIFFPQKSK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Part of a binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.2. The protein should be optimized for ATP-binding cassette (ABC) transporter complex-specific folding and stability, while maintaining inorganic phosphate transmembrane transporter activity.3. The protein contains novel ABC transmembrane type-1 domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "LNNVDLQIGKGERVAIIGPSGAGKSSLLHLMATAVRPSAGQLDLLGEQPWLLTSGARQRLRARVGLVHQAPPLPPRQRVVTAVLAGRLGQWGTLRGLLNLIHPSDVPGAREVLAELGMVDKLFVQCGQLSGGQLQRVGIARALYQQPEVLLTDEPR",
    "response#2": "KGERVAIIGPSGAGKSSLLHLMATAVRPSAGQLDLLGEQPWLLTSGARQRLRARVGLVHQAPPLPPRQRVVTAVLAGRLGQWGTLRGLLNLIHPSDVPGAREVLAELGMVDKLFVQCGQLSGGQLQRVGIARALYQQPEVLLTDEPVSAMDPVLADHSLALLNRHAQAHGVTLVASLHAVELALAHFPRIIGIRAGQ",
    "response#3": "KGERVAIIGPSGAGKSSLLHLMATAVRPSAGQLDLLGEQPWLLTSGARQRLRARVGLVHQAPPLPPRQRVVTAVLAGRLGQWGTLRGLLNLIHPSDVPGAREVLAELGMVDKLFVQCGQLSGGQLQRVGIARALYQQPEVLLTDEPVSAMDPVLADHSLALLNRHAQAHGVTLVASLHAVELALAHFPRIIGIRAGQ"
  },
  {
    "reference": "MEFNVLFISILLVVSAVSAVVGYLVRKSIAEAKISSAEHAAKQIVEDAKREAEAGKKEAILEVKDEVHKLRTEAEREIRERRNEIQKQENRLVQKEEILDRKSETLDKKEESLDKREESLTKKQRQIEEMDSKVEEILNKQQVELERISGFSREEAREVIRAEVEQEMAHETALLIKERVAKAKEEADKKAREILSLAIQRCSADHVAETTVSVVNLPNDEMKGRIIGREGRNIRALETLTGIDLIIDDTPEAVILSGFDPIRREIARTALEKLVQDGRIHPARIEEMVDKARREVDEAIREYGEQTTFEMGIHGLHPDLIKILGRLKFRTSYGQNVLKHSMEVAYLAGLMAAELGEDVKLARRAGLLHDIGKAIDHEVEGSHVEIGVELATKYKEHPVVINAIASHHGDTEATSIISSLVAAADALSAARPGARRETLETYIRRLEKLEEISESFDGVEKTYAIQAGREVRIMVKPDIVDDVLAHKLARDITKKIEEELDYPGHIRVTVIRETRAVEYAK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a specific function within the plasma membrane. The designed protein sequence is ",
    "response#1": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPQ",
    "response#2": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPM",
    "response#3": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPC"
  },
  {
    "reference": "MKKSILDCSIIELTEYLASINEPSFRANQIFEGIYAQNKISFEEFTTLPLNTRQKLAENFYLRTLEKVEEITSTFDNTQKFLWKLIDGYKIESVIITENKRTTFCISSQVGCALDCKFCATGKMGILRNLTPGEIVEQVLLMQEKIGQKPTNIVYMGMGEPMLNYDSVIQAADILTAPKGLGLGKRRITISTSGVIPGIKRFTKEKQPYSLAISLNSIDDKIREKIMPISKKYPINDLMDAARDYTEQLNKLITFEYVLLDKFNASKEDAKKLVQLTHRIPCKINVIPCNSDEPDYQPPSKEKVQIFEQHINERSRRITLRKRKGWEIKAACGQLYAENVKKKRQ",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must have high specificity towards tRNA binding.2. The protein must bind to [4Fe-4S] cluster in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MCPNNALKQCVKEYTVDELMTILKRDFNNWGSDGGVTFTGGDPLMHHEFLVEVLKKCYDSQIHKAIETSGYAKQEVFLEVLKYIDFAFIDVKNMDREKHKQGTGVYNDLILSNIEALKKSNWNGRLVLRQPTIAGYNDSDENAYKLV",
    "response#2": "MCPNNALKQCVKEYTVDELMTILKRDFNNWGSDGGVTFTGGDPLMHHEFLVEVLKKCYDSQIHKAIETSGYAKQEVFLEVLKYIDFAFIDVKNMDREKHKQGTGVYNDLILSNIEALKKSNWNGRLVLRQPTIAGYNDSDENAYKLC",
    "response#3": "MCPNNALKQCVKEYTVDELMTILKRDFNNWGSDGGVTFTGGDPLMHHEFLVEVLKKCYDSQIHKAIETSGYAKQEVFLEVLKYIDFAFIDVKNMDREKHKQGTGVYNDLILSNIEALKKSNWNGRLVLRQPTIAGYNDSDENAYKLN"
  },
  {
    "reference": "MITVIGGTFSKLHKGHKALLNAAIDTGNEVVIGLTSDEYVKKNKVYPAVPYSVRYRALYNFMIKRTKRFRIRQIDDRNGNAPYEKDYEAIVVSPETYPRSLKINEIRVSNGLPPLKIIRVPYVLAQDLFPISSTRIINGEIDTNGRRLAPLKVGISTRNEAKINAVEKFVRRFVKNYQIIKNENYDLKTQQPFNEETMELATKRAMESLRDNDYSVGIESGIIYEKFSKRYYDVHYCVVMDRFGNVTRGMSSGFEISDHIVDRIKHDRTFSDAYSGHFSVTELDQAEGIIGKLSNGKLRRIELIEESIRNAFIPRLDPDFYDSTYTPP",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have a binding site that is specific to the target Mg(2+) ligand.2. We are looking for a protein with broad substrate specificity for H2O + XTP = H(+) + phosphate + XDP.3. We require a protein that can specifically bind to the metabolites produced in the Cofactor biosynthesis pathway.4. The presence of Mg(2+), Mn(2+) is necessary for the protein to function in the desired environment.5. The presence of Cytidyltransferase-like, Non-canonical purine NTP phosphatase/PRRC1 domains is critical for the interaction of the designed protein with its intended targets.6. The designed protein should be involved in coenzyme A biosynthetic process by localizing to cytoplasm and exhibiting pantetheine-phosphate adenylyltransferase activity, ITPase activity, ATP binding.7. For general function, the protein need meet that Reversibly transfers an adenylyl group from ATP to 4'- phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate. The designed protein sequence is ",
    "response#1": "KKILLAVTGSIAVYKTLELVREFIKAGAEVKVVMSESAKKFIAPLTFETLTSNEVLDDTNESWANEHNHIKITQWADIMVIAPATANTIAKLANAIADNMLLQCALAFSGMKILAPSANTNMLQNPITQANLKMLGIANYTILETQTKELACKTIGDGAMAEPLDEALAAITAFIKDKKVVITGGATYENIDDVRAITNFSSGKMSKALSDAFYMLGGNVHFISSVGFKVPYKFTKFKNSFELKTILDKENLQDKDILVMAAAISDYVPKQKFSGKVKKDVFGDKFSLELVKNDDILSNLKVSCKKIGFKMEVDEKTAKQNAKDMLKNKHLDAVCLNVLNDNVKFGSDSTQIIGKAENGTQQTIPLGSKAQLSNHLFDWIVTNF",
    "response#2": "KKILLAVTGSIAVYKTLELVREFIKAGAEVKVVMSESAKKFIAPLTFETLTSNEVLDDTNESWANEHNHIKITQWADIMVIAPATANTIAKLANAIADNMLLQCALAFSGMKILAPSANTNMLQNPITQANLKMLGIANYTILETQTKELACKTIGDGAMAEPLDSPVLYYKVLRGSDNPVKIRAVRSVMKKIYSNVIISASKVENHVGEQPWGEDTLKGAIERAKRAIEDADFGVGIEAGVFEMKDGLFDVQYCAIIDKIGRITIGHGSGFKLPSCIEKDLRNGKTVGKIFEEISETRNIGRKQGAIHFLTKGLMTRQKLTEQCVLAAMVPRGGTFNVLHAGHCALLDATAAIGDEVCIAITSDDFISKTKKYHTPLEKRAERVDQYLSSKKCRYSITVIDTPEGNAPYDKFLDAIVVSPETYENAVKINVSRELNGLKPLEIVTVPHVLAEDGMPISSTRILHNKDINILHGKKVIITAGATIEAIDPVRFLSNHSTGKMGFAIARACVNAGADVTLVAGRAVHLPTPFGAKRLTVGNANDMLTACQSVMTNDSDTIFIATAAVADYKVKSIATQKIKKTSDQDGLTLELIKNPDILATMSHTFPSAIMVGFAAETQDAENYARTKLTAKNLDMIAVNDVSDKSIGFGSDNNAMTLFDESGKHQLLSGSKSEMAGTFNVLHRGHRFLLDTAFSFGNHIVVGITSDAFARQSRESVFPLEERIDRLKQYLSTKNKNWEIEILDDPYGSAISDPDIHILVASERTIEMGKRINEIRESKGLPAIALHAIPLILAEDCVPISSTRI",
    "response#3": "KKILLAVTGSIAVYKTLELVREFIKAGAEVKVVMSESAKKFIAPLTFETLTSNEVLDDTNESWANEHNHIKITQWADIMVIAPATANTIAKLANAIADNMLLQCALAFSGMKILAPSANTNMLQNPITQANLKMLGIANYTILETQTKELACKTIGDGAMAEPLDSGLMRAAIEAALSSDLFLLHGKKVIITAGATIEAIDPVRFLSNHSTGKMGFAIARACVNAGADVTLVAGRAVHLPTPFGAKRLTVGNANDMLTACQSVMTNDSDTIFIATAAVADYKVKSIATQKIKKTSDQDGLTLELIKNPDILATMSHTFPSAIMVGFAAETQDAENYARTKLTAKNLDMIAVNDVSDKSIGFGSDNNAMTVFFADKTEIALPKIKKEDVAKALIEKAISLL"
  },
  {
    "reference": "MVKVVRNVVCPFCGTLCDDLEILVEDNHIVGTRHACRIGNAKFMHFEGAVRYTEPLMRENKKDDFKKVDYETAIEETARLLTEATLPLIYGWSATECHAHMYGVELAELVGAVIDNTASVUHGPSLLAVQDVGYPVCTLGEVKNRADVIIFWGSNPMHAHPRHMSRYSVFARGFFRERGREDRTLIVVDPRETDTAKLADIHLQVEPHKDYELVSAMRAVLKGFELQVDKVAGVPADLIYEAVEVCKNAQFGELFFAMGVTMTRGKHRNIDNAIQLVIDLNAYTKFGLMPMRGHYNVNGFNQVLTWVTGYPFGVDFSRGYPRYNPGETTANDLLQRGETDMMLNIASDPGAHFPQKAVQHMAKIPLVCIDPHETPTTQLANIIIPPAIAGVEVEGTAYRMDGVPIQLRKVIDPPEGVLPDREILKILIKKVKEML",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Catalyzes the reversible oxidation of CO(2) and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR); This enzyme is oxygen-labile.2. A protein that is capable of catalyzing reactions within the One-carbon metabolism, 5,10- methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 1/3, methanogenesis from CO(2) pathway with high efficiency and specificity.3. The protein design should incorporate a binding pocket that specifically recognizes W-bis(molybdopterin guanine dinucleotide) to enhance enzymatic efficiency.4. We need a protein that can perform H2O + N-formylmethanofuran + 2 oxidized [2Fe-2S]-[ferredoxin]   = CO2 + H(+) + methanofuran + 2 reduced [2Fe-2S]-[ferredoxin] with high efficiency. The designed protein sequence is ",
    "response#1": "MTGRHESPLMRENKKDDFKKVDYETATEETARLLVEAKLPLIYGWSSAECHAQQLGVLLAEKTKAIVDNTASVXHGPSLLAVQDVGYPVSTLGETKNRADVVLFWGSNPMHAHPRHMSRYSVFPRGFFRQRGKQDRQMIVVDPRKTDTAKLADIHLQVEPHKDYELVSALRAAAKGFNIEAEQVAGVPTETIYEAVDICKNAQFGSLFFAMGVTMSRGKHRIIDNAIQFVIDMNAYTKFVLTPMRGHYNVNGFNQVSTWVTGYPYGVDFSRGYPRYNPGETASNDVLQRGDTDMMINVASDAGAHFPQKAVQHMAKIPLVCIDPHETPSSVISNIVLPPAITGLEVSGTAYRMDGVPIELRKVIKAPEGMLSDAEIMKMLIKGM",
    "response#2": "MEINGVEIEDTFAEAFGIKVSRVLVTAATKKLAKIAATEATGYGTSVIGCPAEAGIDCYVPPEETPDGRPGYIIMICNPSKKSLDHELLERIGMGILTAPTTAVFDALDDEDEKLNIGFKLKFFGDGYEKELEIDGRKIHSIPNNYVKVVDNPKNKVSEIFDVELKESDVAKVTINNCCKKVKRIGEKMTSGEIVVNGDAGMYIGVEMKGGKITVNGDAESWVGQNLKGGEIIINGNAENYVGSAYRGDWRGMSGGKITITGNAGSELGEYLKGGTIVIKGNTKIMPGIHQNGGMIIIEGDIEGRAGGEMMKGAIVVYGKILEPLPSFKFEGIVEDPLVKLSKKDAGTQLKGTFIKFSGDL",
    "response#3": "MEINGVEIEDTFAEAFGIKVSRVLVTAATKKLAKIAATEATGYGTSVIGCPAEAGIDCYVPPEETPDGRPGYIIMICNPSKKSLDHELLERIGMGILTAPTTAVFDALDDEDEKLNIGFKLKFFGDGYEKELEIDGRKIHSIPTSKICEACMTCELACGEARHESPLMRENKKDDFKKVDYETATEETARLLVEAKLPLIYGWSSAECHAQQLGVLLAEKTKAIVDNTASVXHGPSLLAVQDVGYPVSTLGETKNRADVVLFWGSNPMHAHPRHMSRYSVFPRGFFRQRGKQDRQMIVVDPRKTDTAKLADIHLQVEPHKDYELVSALRAAAKGFNIEAEQVAGVPTETIYEAVDICKNAQFGSLFFAMGVTMSRGKHRIIDNAIQFVIDMNAYTKFVLTPMRGHYNVNGFNQVSTWVTGYPYGVDFSRGYPRYNPGETASNDVLQRGDTDMMINVASDAGAHFPQKAVQHMAKIPLVCIDPHETPSSVISNIVLPPAITGLEVSGTAYRMDGVPIELRKVIKAPEGMLSDAEIMKMLIKK"
  },
  {
    "reference": "MIVLVINSGSSSLKYQLFDMRKEALLAKGLVERIGLSGSMLTHRPIGKDQVVIETEILNHDRAIQLTVEALTHEDHGVVASMREIDAVGHRVAHGGDTFSDSAVVNEQSLANIRALFEVAPLHNPPAVMGIEACQHMLPGVPQVAVFDTAFHQTIPGYAYNYALPYELAAKHSLRRYGFHGTSHKYVAQWAAAMLGRPLEDLRIITCHLGNGASITAIDRGRSVDTSMGFTPLEGLIMGTRVGDMDPAAVPFLMEKEGLTTGQINDLMNKKSGVLGVSGVSSDFRDLEEEAAKGNERCQLALDMFAYRVKKYIGAYAAVMNGVDAIVFTAGLGENSPSMRQSVCSGLSYLGVQLDEEKNKGRGEADISAAGATCRVMVVPTNEELMIARDTYRLLKG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must bind to Mn(2+) in order to perform its enzymatic function.2. The protein design should consider the endogenous proteins and enzymes present in the acetyl-CoA biosynthesis, acetyl-CoA from acetate: step 1/2, Metabolic intermediate biosynthesis pathway to avoid interference with native metabolic processes.3. The designed protein should be involved in organic acid metabolic process, acetyl-CoA biosynthetic process by localizing to cytoplasm and exhibiting acetate kinase activity, magnesium ion binding, ATP binding.4. For general function, the protein need meet that Can also catalyze the reverse reaction.; Catalyzes the formation of acetyl phosphate from acetate and ATP5. The designed protein is the optimization of the Proton donor/acceptor to achieve high catalytic efficiency for acetate + ATP = acetyl phosphate + ADP.6. The protein should be able to bind Mg(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MGYQHTILVLNCGSSSVKYKLFEMPTRRLLASGIVERIGLADSHARLVHKGVEHTRHIPDHEAAIRAMLDYLVEHDVIADPSQIGAVGHRIVHGGPIFTESTIITPEVEAQIQALCDLAPLHNPANLMGIRAFQAALPHARQVAVFDTAFHQTMPPHAYLYALPYRYYREHGVRRYGFHGTSHRHVTERAAELLGRPLEQLKAIICHLGNGGSVSVVRNGRSVDTSMGFTPLAGVTMGTRSGDIDPSIIPYLMQQTGMSADEVNTLLNKESGLLGLSGISSDFRDVEHAAEHGNARAQLALGMFAYRVAKYIGSYTAVLGGLDALVFTAGIGENSSPIRAAICAGLGWLGVRLDREKNEIRGRGEVISARGSDVPVYVIATDEQWEIARETLRAAGIADE",
    "response#2": "MAILAINAGSSTLKFALFDPATGLPLAKGLVERIGEPDSRLRLRVGGEERETTLPIEDHAVAVRRMLDALLAAGIIRPLSEIRGVGHRVAHGGALFRESALVTPEVTAAIEELSELAPLHNPANLVGIREALRVLPGVPHVAVFDTSFHQTMPEHAYLYGLPYDYYQEHGIRRYGFHGTSHQYVTERAAELLGRPLEELKVILCHLGAGASITAVLDGKCVDTSMGLTPLEGLVMGTRSGDVDFGAMAFLMEHERMTAQEVDNALNKKSGLLGLSGISSDMRDIEAAAEEGNERAALALRIFAYRVKKYIGSYTAVLGRLDAIVFTAGIGENSAAVRARVCAGLEFMGVYWDPVKNAERPERLISTPDSRVRVLVVPTNEELVIAQETLRVAGLA",
    "response#3": "MVLAINAGSSSLKYKLFDMPQHQVIAKGLVERIGLKDSVFKIHYKGQQKSEQFDIADHQHAVSIILDALVNAGVINDINEIKGMGHRIVHGGEKFNESVLITEEVIESIEKFIPLAPLHNYANLSGIEAAKEVLPDVPQVAVFDTAFHQTMPKRSYLYALPYKYYQEHGIRRYGFHGTSHRYVSERAAELLGKPLSELKVILCHLGAGASITAVLDGKSVDTSMGFTPLEGLIMGTRCGDLDPAVIPYIMEKTGKSVSEVINMLNKESGLLGISGVSSDMRDIEAAAEQGNEKAQLALEIFVHRIKKYIGAYYAVLNGVDAIVFTAGIGENSALIRARVCQGLEFLGIKMDAEKNEASRGEEGYISTENSPVKVLVIPTNEELVIARETYRLLK"
  },
  {
    "reference": "MIRILVSNDDGVNAPGIKALTEALTEIATVLTVGPDRNCSGASNSLTLTNPLRINRLDNGYISVHGTPTDCVHLAIRELYDGEPDMVVSGINAGANMGDDTLYSGTVAAAMEGRFLGFPAVAISLNGRKFEHYQSAAVYARRIVQGLLAQPLAKDQILNVNVPDLPLDQIKGIKVTRLGARHKAEGIVRTQDPAGREIFWLGPPGQEQDATEGTDFHAIANGYVSITPLTVDLTAYGQLTALQNWVDKI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein can be targeted to cytoplasm and perform nucleotide binding, XMP 5'-nucleosidase activity.2. The protein should have a low Km value for a ribonucleoside 5'-phosphate + H2O = a ribonucleoside +   phosphate. The designed protein sequence is ",
    "response#1": "MLILVTNDDGVHAPGIAALADSLHGLGQVVVVAPDRDRSAIGHALTLHAPLRADELRPGVFAVDGTPTDCVNLGIHGLLSSVPDLVVAGINRGANLGDDITYSGTVCAAMEATLMGVPALAVSLEGDTFASSEYRQAADAALFLAQKVSEEGLPSDTFLNVNVPAGRIRGIRLTRQGRRRYGDMVVEKMDPRGRKYYWLGAGECDFDYVDGTDCHAMHEGFISVTPLHLDLI",
    "response#2": "MDVLLTNDDGIDAVGIRALYDALAEVADVTAVAPADDQSAVGRQLSRTVELHDHELGYAVEGTPADCVIAGLGALDLDPDIVVAGCNEGANLGEYVLGRSGTVSAAVEAAFFGVPAIAASVYFPAGDVTIEEFDPDKTDFAEASRAVRYLVDNAIGAGVFDAADYLNVNAPLPPETGHAPMEITEPSHVYEMDGERDGETVRIQDHIWERMAEGTIPDPPGTDRRAVVEGRVSVSPLTA",
    "response#3": "MRILITNDDGIAAQGIKILEEIAHKISDDVTVVAPDKERSGMAHSLTLSLPSRLYKHDDRHYSVSGTPSDSVVMGIKEIMKDNPPDLVLSGINHGANLAEDVTYSGTVSAAMEGAIAGIRSIGLSQVYSPDFKNGALSFDTAVSWGEKVIRQLIDSTLPNRTFYNVNFPACENDDVKGIRVVAQGHRDYGRLNVEKGVDPRGKDWYWLALGEAISKDNLNGDLDLSYQNYVTVTPLAPNR"
  },
  {
    "reference": "MADEQFPAAATALEEYQSIEEQMASPEVVSNPDKLRKLGRRHAELGAIVDAYKAWLQVKDDLAAAQEMAGEDADFAEEAKRLEDELPGVEEKLRTALIPRDPDDARDTIMEIKAGTGGEEAALFAGDLLRMYTRYAEKRGWSVNVQSENTTELGGVKDVQIAIRAKGTPAPEDGVWASMKYEGGVHRVQRIPVTESQGRIQTSAAGVIVFPEADEDDDEIEIDPKDLKIDIFMSSGPGGQSVNTTYSAVRMTHLPTGITVNMQDEKSQIQNRAAALRVLKSRLLAMKHEQEAAEAADMRHSQVRSLDRSERIRTYNFPENRIVDHRTNYKAYNLDAVLDGDLQAVIDSDIQADEADRLANQK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have translation release factor activity, codon specific and be active in cytoplasm.2. The protein must exhibit the following characteristics: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.. The designed protein sequence is ",
    "response#1": "ELAVEEEDEETFADASSELDALEKRLEELEFRRMFSGPHDISDCYLDIQSGSGGTEAQDWANMVLRMFLRWGEAHDYKPELIEVTDGDVAGIKGATIKFTGEYAFGSLRTG",
    "response#2": "ELLTAEQDAEMRELIREEVKELEAELPDLEQQLKIALIPKDPNDDKNTILEIRAGAGGDEAALFADELFRGYVHYASTQGWKVEMISFSEGNAGGAKEIIASITGDSVFSKMKYIG",
    "response#3": "NESIQENEARMAEPGFWDDQAAAQKVIDENNVLKGKYDTFKQLADEVGDLAVAYELLSEEPDAEMQAEFETDFQHAEHDLQQYRLNLLLDGPYDRNNAILEIHPGAGGTESQDWGAMLLRMYTRWAASHNFKVETVDYQAGDEAGIKSVTLLISGHNAYGYLRSEKGVHRLVRISPFDAAGRRHTSFASR"
  },
  {
    "reference": "MTTLPNLAVSPVFPPAAPSLFGLSRDTLGDRLTAHGVPRYRADQIYAWVYQKHHRSAASMTNLPSGLRSGLEAVCTLDLPRLDTVLGTQDSMTQKFVLELADGARVECVSMRTEKRLTFCVSSQVGCALKCAFCATGLMGLKRNLRAEEIVAQVLAMGDFHRWRDARFNIVFMGMGEPLVNYEPVIESIRILRDERGLNLGARRITVSTSGVVPGIRRLATERMPLGLALSLHATTDELRNELIPLNRRWPLADLIPSVRDYGRAVGRRVTLEYTLLDGVNDLPEDALRLAAIARSLPSKINLIPYNPVPGLPFRRPSPAAVEAFAQQLYPIAPAVTVRNTLGGEIWAACGQLGGLSPTG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be involved in rRNA base methylation by localizing to cytoplasm and exhibiting tRNA binding, rRNA binding, tRNA (adenine-C2-)-methyltransferase activity.2. The presence of [4Fe-4S] cluster is crucial for the protein to function optimally under specific conditions.3. The Proton acceptor, S-methylcysteine intermediate active site of the designed protein must be optimized for substrate binding and specificity.4. The designed protein should have a binding site that is specific to the target [4Fe-4S] cluster, S-adenosyl-L-methionine ligand. The designed protein sequence is ",
    "response#1": "MNIYGMTRYDLEQYFINNNMHKFKALQVFEWLYIHKVKSFFEMTNIKKELQEQLERDFSTRMIHIEKKQQDQETSKYLFKLFDNNYVEAVLMKHDYGLSVCVSSEVGCNMGCAFCESGRLKKVRELNSYEMVEQILLIEQDIGKRVSSIVIMGIGEPLDNYDNVMKFIRIINDPKGLAIGARHITLSTCGLVPKIKELSNLDLQINLAISLHAPNNELRSKIMPINKAYNLNTLIDTIKEYIEKTNRRVTIEYVMLDNINDSEKEAKELSSLIKNLNVYVNLIPYNETNHLEFKRTSKNKIMKFYDYLKKQGINVTIRREFGSNIDAACGQLRSKEVEN",
    "response#2": "KQDRNTICVSSQIGCPLECVFCATGQLGFKRNLTAGEIIEQVLFWGRWLKNDCGAINNVVFMGMGEPFLNYDNVMAAIKIINDKDAFNIGSRHISVSTVGIVEGINKFALEPLQANLAISLHAPDDGLRTQLMPVNKQYPLGEVLSAAVGYIKKTNRKLMFEYLMIKDFNDSEACAKKLAALLKELPQHLIMVNLISYNQTGVFSPSPAAQIKKFKNILMKANIQAIERFKT",
    "response#3": "GRLRRTFCISTQVGCGMGCVFCASGIGGVDRNLSTGEIVEQMLRLQHLLEPDERVSHIVVMGMGEPLANLPALLPALAEASREDGLGISPRRITVSTVGLPAGIRKLAGHDPHYRLAVSLHAPNDELRNQIVPVNDKIGIQAILEAADEYFAASRRRMTFEYVLLGGLNDSPHHAAELARLLRGRETILNVIPYNPVPGLPYQTPTPAAQQAFREVLERGGVAIHFRR"
  },
  {
    "reference": "MNLTLTTTSITIQPSATMANRAPTLLDHFHDQLQKHSSSVAIEDGTQSADQGAWERVTYAQLDALSDSWSKRLRQAGVGAGCIVPLLSKRSVAMVAATLAILKLRAAYVSIDIDSWGKDRIDTVLKTVNPQIIVSTSPCPKDHYPYPVVALERNDFDETVTSNGTQWTRNDEDSIDRGNDLAYIIFTSGTTGIPKGVKIGQRSISRYVKEGGDLPFNFNTTHGTRVLLICSIAFDVCAGVMFNTLCNGGTLVLADPSTFETAAKTCHVLPLTPSILVTLDPKAGFDTVEKIFLGGESPSPSLIEAWSSPRRRLYNAYGPTETTCTAFMGELLPGSPITIGYPISYSTVTLLDEDGMESVEGEICIAGLGLALGYFHDPERTNSAFVEWNGVRIYKTGDYGRRTKHGLQFCGRRDSVVKNRGFLINLEADVEPALLSYDKVDSASAFMSQGQLIAFVTPTSAKEGLREYLANTVSSFLVPDTIYSLDEFPRTSNGKVDRRSLMRMHELEQGSDTASLERGLGAVESVRRGLSHVLRLPESQILPASSFRHLGGHSLAAVMLVSVLRRMGFGISVAEVLLLDTVENIAAAVVELSDIPHALSAQEDLIERLRHDISTTRPLDEGVTIAPMTDMQTRLLGASVATPGLSFIKTSFTLDHPEKEDLTSTLRAAWVRLHQTHEILRTAFVLTASNGTQIISQEPDFSWKEKFVTESEWESVCRREEHLDVADFPDFDAENRASLSRVVLIIAPRRRTRFVWTVHHSLIDGWSM",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Nonribosomal peptide synthesis (NRPS) is a key mechanism responsible for the biosynthesis of bioactive metabolites which are potentially contributing to organismal virulence.2. The designed protein must have at least one Carrier domain.3. The protein should be designed to localize specifically to cytoplasm in order to perform its role in secondary metabolic process, nonribosomal peptide biosynthetic process, secondary metabolite biosynthetic process, amino acid activation for nonribosomal peptide biosynthetic process more effectively. The designed protein sequence is ",
    "response#1": "GEPIAIVSMACRLPGGIATPEELWHLVAEGRDAIGPIPADRGWDLAEVYGPEAGSEGRATPTEGGFLDGAGDFDAAFFGISPREALAMDPQQRHLLELAWETLERAGIDPSGLRTSRTGVYAGASPSGYGEGALATAETAGHSLTGGSMAVLSGRIAYALGLEGPAVTVDTACSSSLVAVHMAVQALRAGECDLALAGGVAVMVQPIAFAEFSRQGGLAPDGRCKPFAAAADGTGWAEGVGLVLLERLSDARRNGHRVLAVLRGSAINQDGASNGLTAPNGPSQQRVIREALANAGLAAADVDVVEAHGTGTRLGDPIEAQAILAAYGAERPAERPLLLGALKSNIGHVQAAAGVAGLIKTVLAIRHGLVPATLHVDAPTPFVDWSSGAVELVAEARPWPASDRERRAAVSAFGIGGTNAHVIVEEAPEPARAEPAGEPARPWVVSARTEPALAAQADRLAEYVRAHPRTEPVAVGWSLAATRAAFEHRAVVLGDGAEQLRQGLAALAAGRPAPGVVTGTADDRRGPVAFTSGSTGKPDRADAAPATPPGTPRPVRLRELAARRPSAEAVRDDAEALDYASLVDRVDAAAARLTAAGAGPGDRVAVLAERGVRAVVAMLACWRAGGVYVPVDPAAPLPRRELILEQAAPAVLVCEDPDEQPPHHRSRAVAIGDLTAEADAGAGTPAEPAPRPHDPAYLMFTSGSTGRPKGVAVSHANLSSFLHALTGRLALGPADRLLALTTTAFDISLLELLGPLVTGGTVVVAPSSAQRGAADLAARLSSPGITTAQATPAVWRLALSAGWRPREGFTLLCGGEALPPDLADLLAATPAEAHNLYGPTETTIWSCAARIRPGEPVTIGRPIPGTRVL",
    "response#2": "PEPIAIVGSSCRFPGSSSSPSKLWDLLREPRDVRRNLSQNLNLQRFYHPSGENHGSTDVKGLGYLLAEDSRLFDPAVFGISPYEAETIDPQQRILLEIVYESMENAGYTLAQMRGSQTSVHVGVMTDDYHDIQLRDVESLPQYTSTGTARSMMANRISYVFDLHGPSVTIDTACSSSLVALHQAVQSLQLGEATCAVVGGVNLIFDPAMYVAESNLHMLSPDSQSRMWDKTANGYARGEGAAAVLLKPLSQALRDNDHIEGLIRGSGVNSDGQSPGITMPTATAQAALIRATYKRAGLDPIKDRCQYFECHGTGTAAGDPVEAQAISEALFEEATFTNEATDTPPLYVGSIKTVTGHLEGCAGLAGLLKALVSIKNRIIPPNMLFNELNPKIEPYYGRMQITTVPIPWPNVGTGAPLRVSINSFGFGGTNSHVILESNPKPRENLPRPVFTGQGAQWPRMGRMLLDHCPLFRDVLERCDATLQALPDHPEWLLVDELCKHAEVSRVGEAAISQPLCTAIQLGLLEVLFSSGIHFDAVVGHSSGEIAAVYACGIISLSAAMQIAFYRGRYASLAQGANGEVGAMMAVGISHREAQKFCQKSEYQGHISVAASNGVQSVTLSGDSEFIQQAKEHFESENVFARLLKVDTAYHSQHMKEKPGQPLAQIFTGQGAQWPRMGAALIESSPAAAKILARLDESLRLLPIRDRPTWSLREKILEGAESSSVAMAFISQPVCAAVQIMLVDMLRAAGIEFSGVLGHSSGEISAAYAAGYLSSEDAIRAAYYRGFHMKSLTQKKGAMIAVGTSYDDAKELCDLPAFEGRVCVAASNSPFSVTLSGDADAIDEVKALMDEEKKFNRLLQVDRAYHSHHMKACAAA",
    "response#3": "HEPVAIVGMACRLPGGVASPEDLWQLVAGDGDAISEFPQDRGWDVEGLYDPDPDASGRTYCRSGGFLHDAGEFDADFFGISPREALAMDPQQRLSLTTAWEAIESAGIDPTALKGSGLGVFVGGWHTGYTSGQTTAVQSPELEGHLVSGAALGFLSGRIAYVLGTDGPALTVDTACSSSLVALHLAVQALRKGECDMALAGGVTVMPNADLFVQFSRQRGLAADGRSKAFATSADGFGPAEGAGVLLVERLSDARRNGHRILAVVRGSAVNQDGASNGLTAPHGPSQQRVIRRALADARLAPGDVDVVEAHGTGTRLGDPIEAQALIATYGQEKSSEQPLRLGALKSNIGHTQAAAGVAGVIKMVQAMRHGLLPKTLHVDEPSDQIDWSAGTVELLTEAVDWPEKQDGGLRRAAVSSFGISGTNAHVVLEQAPPPAGPLTDPAAGGEPIAIVSMACRLPGGIATPEELWHLVAEGRDAIGPIPADRGWDLAEVYGPEAGSEGRATPTEGGFLDGAGDFDAAFFGISPREALAMDPQQRHLLELAWETLERAGIDPSGLRTSRTGVYAGASPSGYGEGALATAETAGHSLTGGSMAVLSGRIAYALGLEGPAVTVDTACSSSLVAVHMAVQALRAGECDLALAGGVAVMVQPIAFAEFSRQGGLAPDGRCKPFAAAADGTGWAEGVGLVLLERLSDARRNGHRVLAVLRGSAINQDGASNGLTAPNGPSQQRVIREALANAGLAAADVDVVEAHGTGTRLGDPIEAQAILAAYGAERPAERPLLLGALKSNIGHVQAAAGVAGLIKTVLAIRHGLVPATLHVDAPTPFVDWSSGAVELVAEARPWPASDRERRAAVSAFGIGGTNAHVIVE"
  },
  {
    "reference": "MLGILAKLFGTKSGRDIKKLQPLVERINEEFQKLHALDDNQLRAQTDKIKGIIDADLSGIDKQIKEHHDKIAANPDLSIDEKEVVFQAIDKLELERNKELEKVLEKVLPQAFAVVKDTARRWKENGKLTVTATPMDIELASRKKNVQIIGSTAEWSSKWLAAGTEVTWEMQHFDVQLIGGMVLHHGKISEMGTGEGKTLVATLPAYLNALARRGVHVVTVNDYLAKRDSEWMAPLFEFHGISVDCIDKYQPNSEERRKAYRADITYGTNNEFGFDYLRDNMATDKDDLVQRGHHYAMVDEVDSVLIDDARTPLIISGPVPKGDQHEFAELKPRIQRVLEEQKKLINTYLVEAKRYIAAGKEKEAGLELLRAHRGYPGYKPLIKQLSETGIKSILQKTENEYMAENNKRMPEVDLPLYFVIDEKHNQVDFTEKGVDFITGEQEDPTLFVLPDIGSELAKIEKDKSISDQERMEQTEKLLSDYSIKQERIHTLQQLLKAYTLFEKDTDYVIMDGKVKIVDEQTGRIMDGRRYSDGLHQALEAKENVRVEEATQTYATITLQNYFRMYHKLSGMTGTAETEEAEFQQIYNLDVVVVPTNRSIARLDEQDKVYKTTREKYNAVADEIVELTEKGRPVLVGTTSVDISELLSRMLKMRNIKHQVLNAKLHAKEADVVAEAGKPGTVTIATNMAGRGTDIKLTAEAKASGGLAIIGTERHESRRVDRQLRGRAGRQGDPGSSQFFVSLEDNLMRLFGSDRIAKFMDRMGYKEGE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be optimized for stability and solubility when expressed in plasma membrane where it will be involved in intracellular protein transmembrane transport.2. We are looking for a protein with broad substrate specificity for ATP + H2O + cellular proteinSide 1 = ADP + phosphate +   cellular proteinSide 2.. The designed protein sequence is ",
    "response#1": "LNGITLPIYENRVTALIGPSGCGKTTLLRCFNRMHDLYANAQYQGEILLYPNQSNILGKDIDPMEVRMRIGMVFQKPNPFPKSIYDNVAYGLTVRGVKKKSFIEEQVEKSLQQASLWNEVKDRLHSSATALSGGQQQRLCIARALATEPEILLLDEPTS",
    "response#2": "KGGKVGLFGGAGVGKTVLMQELIHNIARFHEGYSVVVGVGERTREGNDLWLEMKESGVLPYTVMVYGQMNEPPGVRFRVAHTGLTMAEYFRDVEGQDVLIFIDNIFRFVQAGSEVSTLLGRLPSAVGYQPTLNTDVGEVQERITSTKKGSITAIQAVYVPADDITDPAPWSIFAHLDATTVRYDREDKEAIVNEIATIHGKGNPILVGTESVEVSEKLSRILRQNRIEHTVLNAKNHAQEAEIIAGAGKLGAVTVATNMAGRGTDIKLDNEAVIVGGLHVIGTTRHQSRRIDRQLRGRCARLGDPGAAKFFLSFEDRLMRLFASPKLNTLIRHFRPPEGEAMSDPMFNRLIETAQKRVEGRNYTIRKHLAAGFNRAVKELMEGSQE",
    "response#3": "PGEFTVILGASGSGKSTLLRCLNGLIQPTAGTITIEGYRQINDPKVLHQHRQRTGMIFQQHQLIARQTALQNVLTGRLAYHSTVRSFFPLPKADKYIALECLDRVGLLSKALARVDNLSGGQQQRVGIARALAQQPRLMLADEPVASLDPASSHKVISFLRQICQEDGIAAIMSLHQVDLAKAYADRIVGISQGR"
  },
  {
    "reference": "ATVKVDNSNFQSDVLQSSEPVVVDFWAEWCGPCKMIAPALDEIATEMAGQVKIAKVNIDENPELAAQFGVRSIPTLLMFKDGELAANMVGAAPKSRLADWIKASA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be optimized for protein-disulfide reductase activity.2. The protein must exhibit the following characteristics: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.. The designed protein sequence is ",
    "response#1": "MSKGVITITDAEFESEVLKAEQPVLVYFWASWCGPCQLMSPLINLAANTYSDRLKVVKLEIDPNPTTVKKYKVEGVPALRLVKGEQILDSTEGVISKDKLLSFLDTHLV",
    "response#2": "MGVTPIKSVAEYKEKVTDATGPVVVDFHATWCGPCKAIAPALEKLSETHTGIQFYKVDVDELSEVAASNGVSAMPTFHFYKGGERNEEVKGANPAAIQAGVKAILV",
    "response#3": "MSKGVITITDAEFESEVLKAEQPVLVYFWASWCGPCQLMSPLINLAANTYSDRLKVVKLEIDPNPTTVKKYKVEGVPALRLVKGEQILDSTEGVISKDKLLSFLDTHLK"
  },
  {
    "reference": "MTANLTHLQRLEAESIHIMRGVAANFSKPVMLYSIGKDSSVLLHLAMKAFYPAKPPFPFLHVDTTWKFREMIEFRDRMAAELGFDLLVHINEEGVRDGINPFDHGSNVHTHIMKTVALRQALDKYGFDAAFGGARRDEEKSRAKERVFSFRTASHTWDPKNQRPEMWKIYNTRVAQGESIRVFPLSNWTELDIWQYILQEGIPIVPLYFAKKRPVVNRGGMTIMVDDDRMKLLPGETVEERMVRFRTLGCYPLTGAIDSCAATLEDIVSEMLTARTSERQGRLIDRDEAGSMEKKKREGYF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein with ATP binding functions is required to enable sulfate reduction.2. For general function, the protein need meet that APS synthesis involves the formation of a high-energy phosphoric- sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD. The designed protein sequence is ",
    "response#1": "LEAMLVWMGEQPLVPGQQYQIKQGTQLVNGVVAKVRYQVDINTLRQKGDDPLNLNEIGRVEINVSRPLCFDAYEQNRATGSFVIIDRLSNATVAAGML",
    "response#2": "ITCGSVDDGKSTLIGRLLFDAKMIFEDQLAALEADSKQSGTTGEAMDLALLVDGLAAEREQGITIDVAYRYFSTDNRKFIIADTPGHEQYTRNMATGASTADLAIILIDARKGVLTQTRRHSYIASLLGIRHVVLAVNKMDLVDYSQTTFDAIRQDYQSFAEKLGFSDITAIPISALKGDNILRFLTCGSVDDGKSTLIGRLLHDSKLVYEDTLAALKRDSERHGTTGKGEIDYALLVDGLKAEREQGITIDVAYRYFSTPQRKFIIADTPGHEQYTRNMATGASTCDLAVVLIDARYGVITQTKRHSFIASLLGIRHIVVAVNKMDLVDYDEAVYTKIRDDYLDFSARLGFGDLHFLPISALKGDNVVDRSNAMPWYQGPPLLAHLETVQKSLLRFITCGSVDDGKSTLIGRLLYDTKLIFDDQLAALEKDSRKYGTTGPAIDLALLVDGLEAEREQGITIDVAYRYFATAKRTFIVADTPGHEQYTRNMATGASTAGLAVILVDARKGVLTQTKRHSFICSLLGIKNIVVAINKIDLVDYSHVVFAEIEEAYRDFAEQLDFDSITPIPMSALTGANVTTPSEDLPWYSGPTLLEHLEEVDVDTDRFITCGSVDDGKSTLIGRLLYDSKTIFEDQLAALEADSKRVGTQGGDIDFALLVDGLAAEREQGITIDVAYRFFATSARKYIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSYLAHLVGIRRIVLAVNKMDLVGYDQARFDAIVADYRGFAESIGIEDFTAIPISGFRGDNVTNASANMRWYDGPTLIEHLDTVELD",
    "response#3": "LEAMLVWMGEQPLVPGQQYQIKQGTQLVNGVVAKVRYQVDINTLRQKGDDPLNLNEIGRVEINVSRPLCFDAYEQNRATGSFVIIDRLSNATVAAGML"
  },
  {
    "reference": "MSRPESRSGNASTPQGTSVLKADDGGSGQTPGGSTENSVAYRYVAVQQMTSCRDHIEYQLTVREQPKQSRMCGVGEKADRRPIDPAPIVQLRVVTHDRPIRQSDPVGSASVAPPVERRPGQGAAAPQTPGVRRGLPVTTALGDGWEDKAWYLENPYYFMYAMLCNADTDEELHLLNDGKTRYTSGSCVSCLYHLKDIDGSHQGFFVFPDLSIRVEGRYRLKLCLFETIGHSVHHCKSIYSDPFHVYTAKRFPGMEESTRLSKSFAEQGLKVRVRKHPRSRRRGSKRTKDESDASDETPLARHVSPKRARASDVLPMSGLPMSQPIASSRMPRSMDDRFERADYDRTPSMASAYYDVKGAPPSRHAPWEEEEVLRLRESRAWDPMYDAPSALYSHHAREDRVEDRRLPPRDFADGRYMDGDYPPHTRSAAAPLSSMSARAGPSEYSEHVRSVYLRDDPVRSMHGSPHRLSAALPPLPPPLSKTPSHPALVGRGYPESSASLTPAYSRNYPSVPAPAPAPAPMPMRPAPLLSRSYDHIGQQSSGRIYDDYGPRPNVVAHYSGSYDPRDALARGFSPPSPQPGRRSPYASHHPPTQWRSGPPSPSRPSDYHLGRASAVERGYAAARRSPIPSARGIDTPPEAYAGFESNPFPRRSFGVPDAGRPAATYDAPRAPPADLYAPDEGPPSGRLGGIGFAPSSRERLILPPLPVPSPLSAHRGELTPLGTRDRDREAAYIAMPRDRDQGLAPTERYLSPSGYNPRAGELDQRDRE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should exhibit Polar residues compositional bias.2. For general function, the protein need meet that The complex binds DNA through the DNA-binding domain of vosA that recognizes an 11-nucleotide consensus sequence 5'-CTGGCCGCGGC-3' consisting of two motifs in the promoters of key developmental regulatory genes 3. The designed protein should contain one or more Velvet domains that are essential for its function.4. The protein should be designed to localize specifically to nucleus in order to perform its role in sporulation resulting in formation of a cellular spore more effectively. The designed protein sequence is ",
    "response#1": "CGGCCGCGGCCG",
    "response#2": "CGGCCGCGGC",
    "response#3": "TGGCCGCGGC"
  },
  {
    "reference": "MEESLMINNVRKAIEDLKAGKLIVLIDDDDREAEGDLVGLAELVTGETVNFMAKHGRGLICAPVSTEIAKRLNLHSMLDKNTDPHQTAFTVSVDHKTSTTGISAFERATTIKELANPNSRPEDFNRPGHMFPLIGRDGGIKVRRGHTEASIDLAKLANSAEATYICEIMNEDGTMARRDDLYEFAKKWDLTVVDLDELTRFLSFEDSVKVKLPTEFGDFDLQLFEDEFNKEHLIISKGDLTSEEPLLIRVHSECLTGDVFTSHRCDCGEQLHAALQKISDLGRGAVLYLRQEGRGIGLRNKLLAYQLQEQGVDTYEANVQLGFAPDERDYTIAVDMLDYLGIKSVKLLTNNPDKVEQLTSLGINIVDRVPINIKPHKENKNYLQTKKIKFNHFLDI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The presence of Zn(2+) is necessary for the protein to function in the desired environment.2. The protein should be able to bind Mg(2+), Zn(2+), GTP, D-ribulose 5-phosphate ligand in a variety of conditions3. The protein with the reaction GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate, the Proton acceptor, Nucleophile must be optimized to ensure efficient catalysis.4. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..5. A protein that is capable of catalyzing reactions within the riboflavin biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway with high efficiency and specificity.6. The protein design must be able to perform GTP cyclohydrolase II activity, manganese ion binding, magnesium ion binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, GTP binding in order to contribute to riboflavin biosynthetic process.7. The designed protein have GTP cyclohydrolase II domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "ATATLPLKNFGDFTMTVFNSLLDGSEHFALMKPPAVINRVPLVRIHSECVTGDVLGSCKCDCGAQLEQSLAMLAVEGGVLIYLRQEGRGLGLANKLKAYALQEQGFDTVDANIKLGLPIDNRDYAVAYQILNHFGISILRLLTNNXXXVANLEKYGLKISERETFYRDPKTPGRRERVSSVHLPTIYGDFMAHAYRFILDESPERLHIALVKGDLTAETPVLVRVHSECLTGEVFGSLKCDCGQQLHKAMELIEKEGMGVLLYMRQEGRGIGLLPKLKAYELQEKGLDTVEANLALGYPPDLRDYGVGAQILVHLGLRKIRLMTNNPKKIVGLRGYGLEVVERVPSFPLYNQKMFEAEAKLPTKYGCFKVVGYRNKINGKENLAIVKGNIQNEKEVLVRVHSKCLTGDVFGSLRCDCGNQLSAALKAIEKHGSGVLLYMDQEGRDIGLLNKLKAYELQEKGMDTVEANLELGFPPDLRDYWGAAEILKDLGVKRIKLMTNNPDKIEKLQKYGIDVVKRVSKLPLYNSKMSWEERVSSVHLPTIYGDFMAHAYRFILDESPERLHIALVKGDLTAETPVLVRVHSECLTGEVFGSLKCDCGQQLHKAMELIEKEGMGVLLYMRQEGRGIGLLPKLKAYELQEKGLDTVEANLALGYPPDLRDYGVGAQILVHLGLRKIRLMTNNPKKIVGLRGYGLEVVERVPEFPLYDQKMIWEKVERVTQARIPTVSGAFKAIAYESHDGRTHMALVAGDPAGKEHVLVRVHSECFTGDVLGSTRCDCGVQLSEALERIQQEGEGVLVYIRGHEGRGIGLRHKVEAYALQDGGLDTVEANVELGFAPDTRDYGVGAQILRDLGITTMRLLTNNPTKRAGLEGYGLEIVERI",
    "response#2": "VEVDMPTKYGHFHLIPFLQKSNGLEHIALIKGKWDEDEEILVRVHSSCMTGDIFGSLRCDCGDQIHKAMELIEKEGRGVIVYLMQEGRGIGLMNKMKAYKLQEEGLDTVEANIELGFAADERDYGIGAQILRQLNVRKMRLLTNNPVKRIGLEGYNLQIVGNRVSEAALPTDFGEFHIYAYETDVDSATHVALVKGDVGGRENVLVRVHSECLTGDVFGSLRCDCGSQLHRALEMVNAEGVGVVLYMRQEGRGIGLGNKLKAYHLQEQGLDTVEANIRLGFPADLRDYGIGAQILVDLGLHTIRLITNNPKKVIGLEGYGLEIVGRVPYYSENPKERVSSVHLPTIYGDFMAHAYRFILDESPERLHIALVKGDLTAETPVLVRVHSECLTGEVFGSLKCDCGQQLHKAMELIEKEGMGVLLYMRQEGRGIGLLPKLKAYELQEKGLDTVEANLALGYPPDLRDYGVGAQILVHLGLRKIRLMTNNPKKIVGLRGYGLEVVERVPFYEEPVEEE",
    "response#3": "VSVQMPTKYGNFELIAFKQVNTGEIHMALKKGEWEKGEPVLVRVHSSCVTGDILHSLRCDCGEQLAAAMKMVEKEGKGLILYMNQEGRGIGLMNKLKAYKLQEEGRDTVEANLELGFQMDERDYGVGAQILRHLNICKLRLITNNPRKRAGLLGYGLEVVENVETHIFQDRSNYHRVVETRLPNRYGTWRAIGFAAPHGGGEHLALVHGDLGDGEDVLVRAHSECLTGDVLGSERCDCGTQLDAAMAAIAEEGRGVVLYLRGHEGRGIGLLAKLQAYALQDTGVDTVDANLELGLPADAREYGDAAQMLDDLGVRSARVLTNNPAKLDGLARFGLQVRGRERVTQARIPTVSGAFKAIAYESHDGRTHMALVAGDPAGKEHVLVRVHSECFTGDVLGSTRCDCGVQLSEALERIQQEGEGVLVYIRGHEGRGIGLRHKVEAYALQDGGLDTVEANVELGFAPDTRDYGVGAQILRDLGITTMRLLTNNPTKRAGLEGYGLEIVERIREVEVKMPTEFGVFKAVAYTNDVDNKEHVALVKGEIDSSQPTLVRVHSECLTGDVFHSHRCDCGPQLEAALRQIDSEGSGVLLYMRQEGRGIGLINKLKAYKLQEEGLDTVEANQKLGFAPDLRDYGIGAQILKDLGVSQIKLLTNNPKKIRGLEGYGLEVVERVEEEV"
  },
  {
    "reference": "MNNQECSLYTAEDVRCGEYEIARSLSLEMYTLMERAGQAVFSCILDTFPETHSLLICCGGGNNGGDGYIVASLAKQVGMRVTLWQIGESDLTGDAAAAQAAWLALGGTISLPEDVIPPQYDIIVDALLGTGLHGQVRDSYSGLIQAINNIDIPVLAVDIPSGLCADTGVVLGCAVKAACTVSFIGLKQGLFTGQAAEYTGDIHFAGLGVDKYFSEQVPSVCSLIDAREMKGLFTPRSRTAHKGSNGRALCLGGDRGMFGAIRLASEACARAGAGLTRVVTQPENVPAIVGACPELMVYDWRGNSNEINDHLEWADIIAIGPGLGSSSWGRSLLGYSRTVEKPVVADADCLNLLVKTPDFNNKRIITPHPGEAARLLATTVEEVESDRFKAVQRLQNKYGGVVVLKGAGTLVHNGKHCWVCQAGNPGMATGGMGDVLTGIIAGLLAQGFSLAQAAKAGVWIHSTAADLCAREKGERGMLASELFPYLRPLLNS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein have enhanced (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.2. The designed protein have YjeF N-terminal domains with specific sequence motifs that are crucial for its activity.3. The designed protein should be able to compete with endogenous proteins or inhibitors for AMP binding.4. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.5. The designed protein must be optimized for NADHX epimerase activity, NADPHX epimerase activity. The designed protein sequence is ",
    "response#1": "MKKSDKNTISEGIKGTELMFRAGKGIFESVEWTRSVAIVCGRGNNAGDGYVLAILLQENNIYTKLFLISENFSIEGKYYFDICKERGIPYEIIDSQTSFSSFSIIVDCIFGTGYKGNLEKKERFAIQEINNSNAYVVSVDINSGLNGDNGMADICVISDLTISIGGYKPGHFLNMAKDKIKKLINVDIGINPVDNLPGVLDKLRRLRDPQSYKRTYGHLFIVAGSRRMPGALLMAVKAAVHSGVGLVTVIAPDSVARNCAPHVPEAMWLPCPETSDGWFSVEGKAIILKTLSSASAVLIGPGIGREDETKQLFAQLVRDIKCPIVIDADALMPEIIDALAGGQLGIITPHLGEFNRIRQTADEAYSFEKLASYSKNNPLVTVLKGSITKVAYQGKVYLSPFGGPVLARGGSGDILSGLCGGLLAQQPSEALNAACQAVVLHGSAADAMARERGQTGISTTEIIPYLSKILL",
    "response#2": "MQAIEKEADAAGLTYDQMMQNAGQGLADVVLEVFVEEIEPETVGLVGPGNNGGDTLVAMTALLNEGWKARAYLVKRKKDALVKQFTEAGGEVISGQDAFENLAEAIEVADVLFDGVLGTGIQLPLKKDVAELLSQVNDILDGLEESPLVIAVDCPTGVDCDTGEVAEETIPADLTVTMAAVKQGLVKLPAFEYIGELAVVDIGLPSDLAEGLPARYVVGMGDLISLSRRNSEAHKGNSGRVLVIGGGAYYGAPALAAMAALRTGADIVTVAVPENVADTVASFSPNLIVIPLEGDRLNPSNIPVLETLLETHDVAVIGPGLGRDSATLETVEQLLPICRKAVVDADALFGLKLPVKSEGKFILTPHSAEFSCLSGSSVPKELDSKKAVVGSFASDKAVTVILKGKIDVISDGSVTRLNRTGNAGMAVGGTGDVLTGIIGALFAVNDAIDAALCAVFISGAAGDLAFQEKGNGLLATDIIDRITDVISL",
    "response#3": "LLIAGSEEKMGAAIMATGAALRSGTGLVTAQVPASGATALNICYPEAMLLMRSNTIDNINHYTAIGIGPGLGVNTDSQQILFNVISHYKQPMVIDADALSILAENADWLRLLPKGSILTPHPKEFDRVFGKCVNDFERLHKALALTLQYPLTIVLKGHYTLVAQQGKGFFNTTGNAGLAKGGSGDVLTGIITALLAQKYEPCIAARLGVYLHGTAADLALETEAEESLVATQVATLMENAGRGAAEEVLGLLARRSARRTARVAIVCGKGGNAGDGFVVARWLKRRGCHPLVWLTAPPEEIGGDARVKLAELRRSGLRPIRVGDDAAVASDLRRADLVVDALLGTGARGAPGGLVARVIELINVSGRPVVALDIPSGLPADGGPPAGPAVRATLTVTFAGLKRPW"
  },
  {
    "reference": "MSESLARRAAALDAADELAGARKLFALDDAVYLDGNSLGALPAHVPDRIADVVTRQWGQLRIRSWTEGDWWSAPERIGDRIAPLVGAAPGQIVVGDSTSVNVFKAVVGAVRLSGDPARTDVLVDATTFPTDGYIAESAARMTGRRIVPVAPGELAGALGPATAAVLLNHVDYRSGRLHDLPALTAAVHDAGAVAVWDLCHSAGALPVGLDAHGVDLAVGCTYKYLNGGPGSPAYLYVAHRHQAAFDSPLPGWTSHEDPFAMSPAYAAADGAVRGRVGTPDILSMLALEAALDVWDGVAIETVRAKSLALTDFFLECVAAYVPDGAVTSLTPAAHAERGSQVALRCADAPRVMDELIARGVIGDLRRPDVLRFGFTPLYVGFADAERAARVLGEVLEG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must possess 3-hydroxykynureninase activity, pyridoxal phosphate binding to support tryptophan catabolic process, L-kynurenine catabolic process, anthranilate metabolic process, 'de novo' NAD biosynthetic process from tryptophan under certain conditions.2. The protein must exhibit the following characteristics: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively..3. The protein must bind to pyridoxal 5'-phosphate in order to perform its enzymatic function.4. The protein should be able to bind pyridoxal 5'-phosphate ligand in a variety of conditions5. We need a protein that can perform H2O + L-kynurenine = anthranilate + H(+) + L-alanine with high efficiency.6. The protein should be optimized for activity in Cofactor biosynthesis, NAD(+) biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MYLCGNSLGVQPKRTVTRLNQYLTTWATQGVQGHFKPLEESPLPTWLDADAKAAELIAPVVGANVSEVAVMQTLTANIHLLMSAFYRPDINGRHKIILENKAFPSDHFAVETQIRHHSLSTEKSMVLIESSSKDNIISTEEVLSVISAHADTTALLLLPGIQYYTGQLLDIPAITAFAHKHGIFVIWDLAHAVGNVPLYLHDWGVDAAAWCSYKYLNGGPGCIGGLFVHEPWLEKLAGSNGMKFSPKKGWWPYPNHYPKDFELTGAASITSVCSFMAAIRFFELAGHKDLRKFGRLASLLIKGVMAIEGKHPGYLDHDYIIEDAPYGATICDPRKGYGFFVEDILKYGRLDWAPHMQLGGHDCDDPASYGHEKLVEMFAEGMEKVMEK",
    "response#2": "MRDYQERFAEPLSRIVGAKPSEVTVMNTLTVNLHLLMVSFYQPTKTRYKIICEEKAFPSDQYMFQSQVAFHGYKKEETIIEIKRREGEHNIRLEDILAKINEVGDELALVLIGGVNYYTGQVFDMKTITEAGHKVGAKVGFDLAHAAGNIKLDLHDWNVDFAAWCSYKYLYNDKLLVGAKQNEVAVMGSLTSNLNALLIHFYKPKGKRTKILFEKQAFPSDYYAFLNIVQVFGYDASHLIQIEIPKGETYIKTETILDVFDKYEDEIAIVCLPGIQYYTGQFFDIAKITKHVKTSAPDVVVGWDLAHAVGNVPLSLHDWGVDFAAWCSYKYLNAGPGAIAGIFVNEKYTEQNKPENF",
    "response#3": "MTDYQERFAEPLSRIVGAKPSEVTVMNTLTVNLHLLMVSFYQPTKTRYKIICEEKAFPSDQYMFQSQVAFHGYKKEETIIEIKRREGEHNIRLEDILAKINEVGDELALVLIGGVNYYTGQVFDMKTITEAGHKVGAKVGFDLAHAAGNIKLDLHDWNVDFAAWCSYKYLVGAKQNEVAVMGSLTSNLNALLIHFYKPKGKRTKILFEKQAFPSDYYAFLNIVQVFGYDASHLIQIEIPKGETYIKTETILDVFDKYEDEIAIVCLPGIQYYTGQFFDIAKITKHVKTSAPDVVVGWDLAHAVGNVPLSLHDWGVDFAAWCSYKYLNAGPGAIAGIFVNEKYTEQNKPENL"
  },
  {
    "reference": "SESILIVHQQQSRSSGS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that May have antimicrobial activity. The designed protein sequence is ",
    "response#1": "GLVVILAAQLLVVLDGTVVNIALPSVQRDLGMSDTSRQWVITAYTLAFGGLLLLGGRVADAFGRRRIFAVGILGFGLASLLGGAAPDPGTLFLARALQGVFAAALAPAALALINTLFTEPGERGKAFGVYGAVSGGGAAVGLLAGGLLTEYLDWRWCLYVNAPVALLALLGCRLLPRDRRTGRAVRLDLPGTLLGCGGLVAIVYAFAEAESGWGDPLVVRLLVLGVLMLVAFALVERRVQDPLLPPGVVAHRVRGGSFLVVGLPQIGLFGLFLFLTYYLQGILDYSPVLTGVAFLPLGLGIAVGSSLIAARLLPRTRPRTLIVGALLAAAAGMALLTRLEPDTPQVYLTHLLPAQILIGLGIGCMMMPAMHTATARVAPHEAGAAAAVVNSAQQVGGALGVALLNTVSTGATAAYLADHGTSPAATVDGTVHGYTVAIAFAVGVLLLTAVLAWVLIDSRR",
    "response#2": "GLVVILAAQLLVVLDGTVVNIALPSVQRDLGMSDTSRQWVITAYTLAFGGLLLLGGRVADAFGRRRIFAVGILGFGLASLLGGAAPDPGTLFLARALQGVFAAALAPAALALINTLFTEPGERGKAFGVYGAVSGGGAAVGLLAGGLLTEYLDWRWCLYVNAPVALLALLGCRLLPRDRRTGRAVRLDLPGTLLGCGGLVAIVYAFAEAESGWGDPLVVRLLVLGVLMLVAFALVERRVQDPLLPPGVVAHRVRGGSFLVVGLPQIGLFGLFLFLTYYLQGILDYSPVLTGVAFLPLGLGIAVGSSLIAARLLPRTRPRTLIVGALLAAAAGMALLTRLEPDTPQVYLTHLLPAQILIGLGIGCMMMPAMHTATARVAPHEAGAAAAVVNSAQQVGGALGVALLNTVSTGATAAYLADHGTSPAATVDGTVHGYTVAIAFAVGVLLLTAVLAWVLIDSRR",
    "response#3": "GLVVILAAQLLVVLDGTVVNIALPSVQRDLGMSDTSRQWVITAYTLAFGGLLLLGGRVADAFGRRRIFAVGILGFGLASLLGGAAPDPGTLFLARALQGVFAAALAPAALALINTLFTEPGERGKAFGVYGAVSGGGAAVGLLAGGLLTEYLDWRWCLYVNAPVALLALLGCRLLPRDRRTGRAVRLDLPGTLLGCGGLVAIVYAFAEAESGWGDPLVVRLLVLGVLMLVAFALVERRVQDPLLPPGVVAHRVRGGSFLVVGLPQIGLFGLFLFLTYYLQGILDYSPVLTGVAFLPLGLGIAVGSSLIAARLLPRTRPRTLIVGALLAAAAGMALLTRLEPDTPQVYLTHLLPAQILIGLGIGCMMMPAMHTATARVAPHEAGAAAAVVNSAQQVGGALGVALLNTVSTGATAAYLADHGTSPAATVDGTVHGYTVAIAFAVGVLLLTAVLAWVLIDSRR"
  },
  {
    "reference": "MSTPTDSKAPLRQVKRVQFGILSPDEIRRMSVTEGGVQFAETMEGGRPKLGGLMDPRQGVIDRTSRCQTCAGNMTECPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYCSKMLVSPHNPKIKEIVMKSRGQPRKRLAYVYDLCKGKTICEGGEDMDLTKENQQPDPNKKPGHGGCGHYQPSIRRTGLDLTAEWKHQNEDSQEKKIVVSAERVWEILKHITDEECFILGMDPKYARPDWMIVTVLPVPPLAVRPAVVMFGAAKNQDDLTHKLSDIIKANNELRKNEASGAAAHVIQENIKMLQFHVATLVDNDMPGMPRAMQKSGKPLKAIKARLKGKEGRIRGNLMGKRVDFSARTVITPDPNLRIDQVGVPRSIAQNLTFPELVTPFNIDRMQELVRRGNSQYPGAKYIVRDNGERIDLRFHPKSSDLHLQCGYKVERHLRDDDLVIFNRQPTLHKMSMMGHRVKVLPWSTFRMNLSCTSPYNADFDGDEMNLHVPQSMETRAEVENIHITPRQIITPQANKPVMGIVQDTLTAVRKMTKRDVFITREQVMNLLMFLPTWDAKMPQPCILKPRPLWTGKQIFSLIIPGNVNMIRTHSTHPDEEDEGPYKWISPGDTKVMVEHGELIMGILCKKSLGTSAGSLLHICFLELGHDIAGRFYGNIQTVINNWLLFEGHSIGIGDTIADPQTYNEIQQAIKKAKDDVINVIQKAHNMELEPTPGNTLRQTFENKVNRILNDARDKTGGSAKKSLTEYNNLKAMVVSGSKG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Mg(2+), Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.2. A protein that can perform DNA binding, DNA-directed 5'-3' RNA polymerase activity, metal ion binding in RNA polymerase II, core complex, nucleus, polytene chromosome puff, polytene chromosome to support transcription by RNA polymerase II.3. The protein should have a low Km value for a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +   RNA(n+1). The designed protein sequence is ",
    "response#1": "EWMVLCLLPVLPPELRPIIQVDGGKLMSSDINELYRRVIYRNNTLIDLLTTSRSTPGELVICQEKLVQEAVDTLLDNGIRGQPMRDGHNKVYKSFSDIIEGKEGRFRETLLGKRVDYSGRSVIIVGPSLSLHRCGLPGEIAIELFQTFLIRGLIRKHFASNIGIAKSKIRQKEPIVWEILQEVMQGHPVLLNRAPTLHRLGIQAFQPILVEGRAICLHPLVCKGFNADFDGDQMAVHVPLSLEAQAEARLLMFSHTNLLSPAIADPISVPTQDMLIGLYILM",
    "response#2": "QIVSPQSNKPCMGIVQDTLCGIRKLTLRDTFIELDQVLNMLYWVPDWDGVIPTPAIIKPKPLWSGKQILSVAIPNGIHLQRFDEGTTLLSPKDNGMLIIDGQIIFGVVEKKTVGSSNGGLIHVVTREKGPQVCAKLFGNIQKVVNFWLLHNGFSTGIGDH",
    "response#3": "KWMVLCLLPVLPPEPRPIVQLGEGGLITSSDLNELYRRVINRNNTLTNLLARSGSESFVIYQTKLIQEAVDALLDNGICRQPMRDSHNRPYKSFSDVIEGKEGRFRENLLGKRVDYSGRSVIVVGPFLSLYQCGLPSEIAIELFQAFLIRSLIGRHIAPNLRTAKSMIRDKGPIVWEVLQEVMQGHPILLNRAPTLHKLGIQAFQPILVEGRAIRLHPSVCGGFNADFDGDQMAVHVPLSLEARAEARLLMFSETNLLSPAIGDPISIPTQDMLLGLYISLAXNNILSPASGKPLAMPRLDMVTGLYYLTLEKSSEEFGGQGAYQPADENGPEKGVYSSLAEAIMAYDRGVLGLQAPVRIRLNHLRPPAEVEAEQFPDGWNQGETWLAHTTLGRVMFNEILPWNYPYLEGVMVRKGGGSDKIMLGDVVNDLAAKYPMITVAQTMDKMKDAGFYWSTRSGVTIAMSDLVIPPIKTQIVSPQSNKPCMGIVQDTLCGIRKLTLRDTFIELDQVLNMLYWVPDWDGVIPTPAIIKPKPLWSGKQILSVAIPNGIHLQRFDEGTTLLSPKDNGMLIIDGQIIFGVVEKKTVGSSNGGLIHVVTREKGPQVCAKLFGNIQKVVNFWLLHNGFSTGIGDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX"
  },
  {
    "reference": "MLTRNMTKAVRTNTSAPSAKQQTAAVLKQKAQQIVKAECDHDVNRSKILGERHHHSTKLKTAVSQAHQTQARPTEETGNVQVRPRRKSIKVEYDSTAGTRDKELQDSNNPPKSRWEPQDWIKQLDNIREMRKNKDAPVDQMGAGKCFDPRAAPQVIRYQVLISLMLSSQTKDEVTAAAMKKLQAHGLSVENIVQTDDKTLGELIYPVGFWKSKVKYMKQCSEILQKQYNGDIPDNVTDLVKLPGIGPKMAHLTMAIAWNTVSGIGVDTHVHRIANRLRWMKRETKTPEETRVGLEDWLPRDLWSEINWLLVGFGQQICQPVYPRCSDCLNRGICPSANLKKTKGR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have a high affinity for [4Fe-4S] cluster.2. The protein have enhanced 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-   deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-   dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-   2'-deoxyribonucleoside-DNA + H(+).3. The designed protein should contain one or more HhH-GPD domains that are essential for its function.4. The protein should exhibit Polar residues compositional bias.5. The protein should be optimized for nucleus-specific folding and stability, while maintaining oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity, endonuclease activity, 4 iron, 4 sulfur cluster binding. The designed protein sequence is ",
    "response#1": "LSSQTKDQVTAAAIANLRARLPGGLTLKSVLECDANLLQSCICKVGFHSRKTDYIKQAARICQEQHGGDIPDTIEGLMGLPGVGPKMGYLALQCAWNKNAGIGVDVHVHRITNRLGWCDTEKGGPEATRKALESWLLPTHWQAINPLLVGFGQLSSQTKDQVTAAAMQRLRAHGLTVETVLKMDDDTLGQLIYPVGFWKTKVKYLKRATEIIHEEFGGDIPSTVEALVRLPGVGPKMAHLTMAIAWNQVSGIGVDTHVHRISNRLGWTRTQTKNPENTRQALEGWLPKETWCEINWLLVGFGQV",
    "response#2": "LSSQTKDGVTFAAMDRLKKHGLTIPSIFETSESVIEELIYPVGFWKKKAAFIKNATAICHDKFNNDIPNSLQGLLSLPGVGPKMAHICMNAAWGVVTGIGVDTHVHRIANRLKWVNTKKPEETRNCLEALLPRCEWDDINILLVGFGQGPRPAPPPRLSSQTKDEITYEAMQRLRSTSLTPQAIIDIDSVELENLLKPVSFYKNKAKYLQLASKVLVDKYDSDIPGTIKELQTLPGVGPKMAHICMATAWNEVTGIGVDVHVHRIANRLKWLPKPTKEPEQTRVGLEAWLPREMWQDVNHLLVGFGQGPGPRPVPPPQAIVPPPATAPPPAVAPPPAVAPPAVAPPAVAPPAVAPPAIAPPAVAPPAVAPPAIAPPAIAPPAIAPPAIAPPAIAPPAIAPPAIAPPAIAPPAIAPPAIAPPAIAPPAIAPPAIAPPAVAPPAIAPPAIAPPAIAPPAIAPPAIAPPAIAPPAIAPPAIAPPAIAPAIAPPAIAPPAIAPPVIAPQAVAPPVIAPQAIAPPVIAPQAIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVIAPQVITPQVIAPQVIAPQVIAPQVI",
    "response#3": "LSSQTKDGVTFAAMDRLKKHGLTIPSIFETSESVIEELIYPVGFWKKKAAFIKNATAICHDKFNNDIPNSLQGLLSLPGVGPKMAHICMNAAWGVVTGIGVDTHVHRIANRLKWVNTKKPEETRNCLEALLPRCEWDDINILLVGFGQLSSQTKDQVTFAAMERLRKRGLTVDSVLSMSDDELGKLIYPVGFWKTKVKYIKKTTQTLKEQYNGDIPDSAEKLCKLTGVGPKMAHICMLVAWNKVTGIGVDTHVHRISNRIGWVKKTTNTPEDTRKALQTWLPFELWSEVNHLLVGFGQL"
  },
  {
    "reference": "MDDIRFAVLGTGGIGRRTLEVSQHKDGLTAVAACDRNGVALDHDGLDVDELLEATEGNIASGPQSGPEQSSGDEPRDSGPEDDPDMAADGGTATAETADGVKQHGEQAGIVASSQGEPTETPIDDIIAESDAINAVLLALPNLEHDFIPRVAERFAEADYEGVMIDVLKRSRVIGMLDDREDLLKESGITFVCGAGATPGFLTGAAALAAQSFVEVEEVEIWWGVGLKSGYEDNRGTVREDIAHLDGYDIETAREMSEAEIEALIDEHDGVLEFHDMEHADDVLLERAGICDAEDVHVGGVLDVRSDEKPTTTTVSVTGTTFDGETGTNTFELDDATSMEANVNGPALGYLKAGVRNNRAGHYGVFGPADLMPGF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: A 'suicide' enzyme that participates in biotin synthesis.2. The protein design must be able to perform oxidoreductase activity, NAD binding in order to contribute to biotin biosynthetic process.3. The desired protein should be able to perform CO2 + H2O + N(6)-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-   lysyl-[protein] + NADP(+) = (7R,8S)-8-amino-7-(carboxyamino)nonanoate   + (S)-2-amino-6-oxohexanoyl-[protein] + 3 H(+) + NADPH4. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.5. The designed protein should have a binding site that is specific to the target NAD(+) ligand. The designed protein sequence is ",
    "response#1": "MMQAKRIGVLGFGGLGQAAAKVLAPKKEMILVTVADKKGYAYSPEGLNAKECIATYHSQGSVGYLDIFGTLTNYSIQDLIESGHPVDGYFLALPNLPNDFIASVAQQFIKSIEKAADFYKKQKTQAKRIGVLGFGGLGQAAAKVLAPKKEMILVTVADKKGYAYSPEGLNAKECIATYHSQGSVGYLDIFGTLTNYSIQDLIESGHPVDGYFLALPNLPNDFIASVAQQFIKSTEKAADFYKKQKTKMFVGVGVLGFGGLGQAAARVLTPKQEMCWVAAADQKGYIYSPEGLDPDLCIRTYRDRGSVADIELQAKRIGVLGFGGLGQAAAKVLAPKKEMILVTVADKKGYAYSPEGLNAKECIATYHSQGSVGYLDIFGTLTNYSIQDLIESGHPVDGYFLALPNLPNDFIASVAQQFIKSTEKAADFYKKQKTKMFVGIGILGFGGLGQAAARLLNQKQELRWVAAADRAGYAYDPKGLDTDVCIAATRERGSLGSVPLNQAKRIGVLGFGGLGQAAAKVLAPKKEMILVTVADKKGYAYSPEGLNAKECIATYHSQGSVGYLDIFGTLTNYSIQDLIESGHPVDGYFLALPNLPNDFIASVAQQFIKSTEKAADFYKKQKTKMFVGF",
    "response#2": "MPKVGILGFGGLGQAALKLLSTKQEMLCVALADQKGYVYASQGIDVNKAIATYQAKGSVGYLESGVLRRVGVLGFGGLGQAAAKILAAKQEMTLVAAADHKGYVYRSAGLDIDAAISAYDKHHSVGYLATGGVLRRVGVLGFGGLGQAAAKILAAKQEMTLVAAADHKGYVYRSAGLDIDAAISAYDKHHSVGYLATGGVLR",
    "response#3": "MSQAKRIGVLGFGGLGQAAAKVLAPKKEMILVTVADKKGYAYSPEGLNAKECIATYHSQGSVGYLDIFGTLTNYSIQDLIESGHPVDGYFLALPNLPNDFIASVAQQFIKSLNLNDFNQQQFQAKLYNNQAKRIGVLGFGGLGQAAAKVLAPKKEMILVTVADKKGYAYSPEGLNAKECIATYHSQGSVGYLDIFGTLTNYSIQDLIESGHPVDGYFLALPNLPNDFIASVAQQFIKSVFNINKYNHQDIIGILGFGGLGQAAARLLNQKQELRWVAAADRAGYAYDPKGLDTDVCIAATRERGSLGSVPATLKVGILGFGGLGQAALKLLSTKQEMLCVALADQKGYVYASQGIDVNKAIATYQAKGSVGYLESGVLNQAKRIGVLGFGGLGQAAAKVLAPKKEMILVTVADKKGYAYSPEGLNAKECIATYHSQGSVGYLDIFGTLTNYSIQDLIESGHPVDGYFLALPNLPNDFIASVAQQFIKSVFNLNKFN"
  },
  {
    "reference": "MPSVALNSPRLRRVFVVGVGMTKFMKPGGENSRDYPDLAKEEIQRGRAALQLLYVYTLGVQVLCFSVGESTCGQRAIYHSLGLTGIPIINVNNNCSTGSTALFMAQQLVQGGLANCVLALGFEKMEKGSLGTKYSDRSNPLEKHIDVLINKYGMSACPFAPQLFGSAGKEHMETYGTKVEHFAKIGWKNHKHSVNNPYSQFQDEYSLDEIMKSRPVFDFLTVLQCCPTSDGAAAAIVSSEEFVQKHGLQSKAVEIVAQEMVTDMPSTFEEKSVIKMVGYDMSKEAARKCYEKSGLGPSDVDVIELHDCFSTNELLTYEALGLCPEGQGGALVDRGDNTYGGKWVINPSGGLISKGHPLGATGLAQCAELCWQLRGEAGKRQVPGAKVALQHNLGLGGAAVVTLYRMGFPEAASSFRTHQISAAPTSSAGDGFKANLIFKEIEKKLEEEGEEFVKKIGGIFAFKVKDGPGGKEATWVVDVKNGKGSVLPDSDKKADCTITMADSDLLALMTGKMNPQSAFFQGKLKIAGNMGLAMKLQSLQLQPDKAKL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should contain one or more Thiolase C-terminal domains that are essential for its function.2. The ideal protein would be one that plays a critical role in negative regulation of cell migration through collagen binding, lipid binding within mitochondrion.3. We need a protein that can perform propanoyl-CoA + tetradecanoyl-CoA = 3-oxo-2-   methylhexadecanoyl-CoA + CoA with high efficiency. The designed protein sequence is ",
    "response#1": "ILQQAMNEDEEGLVERHRGIYGLKVVKKSGETGYWVINTKVGKGQIVFNGKDKPDVTFIVKEEDVLELLSGKIPPQKAFFQGKVKIQGNMGVAMRLIELL",
    "response#2": "SLKENGEDLVKQAGGVFQFNVTKGPGGKQDTWIVDAKHGRGSVTRGGTVTGDVTLTMADKDLVSLLEGRLNAQKAFFQGLVKIKGNMALAIKLQTFQG",
    "response#3": "ILQQAMNEDEEGLVERHRGIYGLKVVKKSGETGYWVINTKVGKGQIVFNGKDKPDVTFIVKEEDVLELLSGKIPPQKAFFQGKVKIQGNMGVAMRLIELL"
  },
  {
    "reference": "MKDLGAEHLAGHEGVQLLGLLNVYLEQEERFQPREKGLSLIEATPENDNTLCPGLRNAKVEDLRSLANFFGSCTETFVLAVNILDRFLALMKVKPKHLSCIGVCSFLLAARIVEEDCNIPSTHDVIRISQCKCTASDIKRMEKIISEKLHYELEATTALNFLHLYHTIILCHTSERKEILSLDKLEAQLKACNCRLIFSKAKPSVLALCLLNLEVETLKSVELLEILLLVKKHSKINDTEFFYWRELVSKCLAEYSSPECCKPDLKKLVWIVSRRTAQNLHNSYYSVPELPTIPEGGCFDESESEDSCEDMSCGEESLSSSPPSDQECTFFFNFKVAQTLCFPS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be involved in cell division, regulation of cyclin-dependent protein serine/threonine kinase activity, mitotic cell cycle phase transition by localizing to cytoplasm, cyclin-dependent protein kinase holoenzyme complex and exhibiting cyclin-dependent protein serine/threonine kinase regulator activity.2. For general function, the protein need meet that May play a role in growth regulation and in negative regulation of cell cycle progression. The designed protein sequence is ",
    "response#1": "PTPKTFLRRFLRAAQASRLSPSLEVEFLASYLTELTLIDYHFLKFLPSVVAASAVFLAKWTMDQSNHPWNPTLEHYTTYKASDLKASVHALQDLQLNTKGCPLSAIRMKYRQEKYKSVAVLTRL",
    "response#2": "VTPFTFIDFFACKHISNAMVQNANSDIQFLDHCPSSMAAAAVLCATGETPSLAFVNPELAVNWCIGLAEEGISSCYQLMQQLVIGNVQRSAAAAAAVNLFSDEGLSYDSSSPPPR",
    "response#3": "PNPLNFIRRISKADNYCIETRNMAKFIMEYSICCNKFIHLKPSYLAAMSMYIARKIKNENSKWDETFIHYSGGIDIESDPAFKDFISELVEDIAVPDTNLDSLRLKYKKPKHGMVYFKVFN"
  },
  {
    "reference": "MVTSINKLKFQETIKKQFSISGIGLHTGKKCNLTFKPAPSDFGIKFKRVDISGSPEIKAHIDNVIDTTRGTTIGNDLFQIHTVEHLLAAASGLKIDNLLIEIDNIEVPILDGSSKPFVDSFIKSGFKELDSHRVELIIDEPIIYSNPKSGIDIHIVPSDKFRITFMMDYKHPSLGTQYTSYYSIRDKFINEVAPARTFCLLSEVNNLLDLGIIKGGSLDNSLVFSDTSLSSVQIKQIVDLLDINEDNISTASGNLVNDTKLRFYNEPVRHKLLDLIGDLSLLGFPIKGHVIAARSGHHANVEFAKKISESYFKKGVNYKMVNNKKNISFDIKEVLNILPHRYPFLLIDRITNLNPGKEVHALKNVTINEPFFQGHFSGQPVMPGVLLIESMAQAGGFLVLNSIPNPESKLIYISGIDDTRFKQVVVPGDQLTIKAKLLKFKLNTCKISADIHVNKKLVAQSTFMASVVNRNT",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein's active site, Proton donor must be carefully engineered to ensure that the amino acids involved in a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine +   H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate are properly positioned for optimal catalysis.2. The designed protein should have 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity, (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity, UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity, UDP-3-O-acyl-N-acetylglucosamine deacetylase activity to facilitate fatty acid biosynthetic process, lipid A biosynthetic process.3. A protein that can modulate the overall flux of metabolites through the Glycolipid biosynthesis, lipid IV(A) biosynthesis, lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 2/6 pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MNNEIQTNKNNVIEAHNLSKQFPGVQALSDVSLDLYRGEIHALLGENGAGKSTLMKILSGVYKPDSGEIYLNNQKVEISSPLDAQKNGISTIYQELNLVPHLTVAQNIFLGREPKRFGFLDWKKMNEETEKLLKSFDLKLNPKTPVSNLSGGQRQTVEIAKALMFNAKIVILDEPTAALSIKETEHLLNLIRTLKQKGLTIIFITHNLHHVFNIADRVTVLRLGKKVGEFFTKETNQEKLVRLITGQEIEEIFPEKKQAHPMK",
    "response#2": "MSHRYRHGSQPKTSPVSATPLSELGLTARGGREARVXRXSAXDLXGAXRQIFGDXLLXAAEXAXDXAIVAPXLEXVXGAXASXAAXAAXAMFXDAXTXXAAXAXLVXAXAXDXXLXADXDXXIVXSXKXXNXPXAAXTXXPXPXQXAXSXAXXXAXAXHXHXTPXXXA",
    "response#3": "MNTNKNLYQQSIEWVKSNSKLIAKGKIVDPKLKAIKDEIXKXDXCXGYIXDPXDXLXDXXIXRXNXXSXSXIXDEXTXLXEXIWNAAXLXSSLVSGYXTPXKSYLVXAXDXYXNXXKXSXXI"
  },
  {
    "reference": "MLTYPNIDPVAIHLGPLQVHWYGLMYLLAFLCAWGLASYRAKQRDGWTSDMVSDLVFYGALGVVLGGRIGYVLFYEFDKFLENPIWLFQVWTGGMSFHGGFLGVMIAMLFWCKKYQKTWFQTLDFVAPCVPTGLMFGRIGNFIGGELYGRAVTDPNYPFGMIFPTDPLHLVRHPSQIYQALCEGLLLFIILWWFSSKPRPRMAVSALFLMGYGVARFVMEFFRQPDADQGFILFGWMTKGQILTVPMLLIGLWMMWYAYQKKIYDWGPQKNS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that can modulate the overall flux of metabolites through the lipoprotein biosynthesis (diacylglyceryl transfer) pathway, enhancing or suppressing its activity as needed.2. A protein that contributes to lipoprotein biosynthetic process by functioning in plasma membrane with phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity.3. The designed protein should have a high specificity for 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) binding, with minimal off-target effects.4. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "GHYSL",
    "response#2": "LIFSVL",
    "response#3": "MSGLPAR"
  },
  {
    "reference": "MDPRPLFQAVDALCDENISHFAGSGSDVGSRLVQAFSRIQEQAKGIEPALRALAAVCPLFDLDPNTPANGYRSLLKVVLSCTQHILHKCRYVTASRRSLFFRAVHNCAELEAYGAALIQLRALLCFAQRLLTANQHGDLFFWQEHGLSEEFLREYSTMHKGCFYGRCLGFQFTPSIRPFLQTISIGLVSFGENYKRHESGISVAASSFFISGRYAIDPELRGAEFERITQNLDVNFWKAFWNITEMEVLTSLVGMASNNVRINKCLSVPPEPLEMPLVSDPQCTVTISPPVAHTGPGPVHMRLISYQLREGQESEQLSALVKPEGPLSIDLRLRSRTAPLSPDLIIHFHGGGFVAQTSKSHEPYLRTWAQDLEAPILSIDYSLAPEAPFPRALEECFYAYCWALKNCRLLGSTAERVCLAGDSAGGNLCITVCLRAASVGVRMPDGIMAAYPATLLQATASPSRLLTLMDPLLPLSVLSKCLSAYAGTERVPVNDRAVEKQSTVSLVKRGTALFLRDIRQGATSWLSSLMDGRKENPETVRKSVSEAALSEEGVSHGRASLKSKTCQDLSSHQYIEQNGSLNHPHSRTQNKQVKDHEQNGLGNCISPGESSQMPLSSLPVQPDQDNTEQLFPRSPQRGTFFLPESEGESDSPKKITETENSSHCKENVPPSHSSPFSSTSHPMGFPEGFEPLRSKNGVAHMTLQTSAIVHNPYMSPLLAPDSMLQGLPPIHIVACALDPMLDDSVMFAKRLRSLSRPVTLQVVENLPH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein should be able to manipulate the activity of enzymes in the Lipid metabolism pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "AGDTDIHALLPESSRRAYDVRPIVTAILDADTPFDEFQANWAPSMVVGLGRLSGRTVGVLANNPLRLGGCLNSESAEKAARFVRLCDAFGIPLVVVVDVPGYLPGVDQEWGGVVRRGAKLLHAFGECTVPRVTLVTRKTYGGAYIAMNSRSLNATKVFAWPDAEVAVMGAKAAVGILHKKKLAAAPEHEREALHDQLAAEHERIAGGVDSALDIGVVDEKIDPAHTRSKLTEALAQAPARRGRHKNIPY",
    "response#2": "AGDTDIHALLPESSRRAYDVRPIVTAILDADTPFDEFQANWAPSMVVGLGRLSGRTVGVLANNPLRLGGCLNSESAEKAARFVRLCDAFGIPLVVVVDVPGYLPGVDQEWGGVVRRGAKLLHAFGECTVPRVTLVTRKTYGGAYIAMNSRSLNATKVFAWPDAEVAVMGAKAAVGILHKKKLAAAPEHEREALHDQLAAEHERIAGGVDSALDIGVVDEKIDPAHTRSKLTEALAQAPARRGRHKNIPE",
    "response#3": "AGDTDIHALLPESSRRAYDVRPIVTAILDADTPFDEFQANWAPSMVVGLGRLSGRTVGVLANNPLRLGGCLNSESAEKAARFVRLCDAFGIPLVVVVDVPGYLPGVDQEWGGVVRRGAKLLHAFGECTVPRVTLVTRKTYGGAYIAMNSRSLNATKVFAWPDAEVAVMGAKAAVGILHKKKLAAAPEHEREALHDQLAAEHERIAGGVDSALDIGVVDEKIDPAHTRSKLTEALAQAPARRGRHKNIPG"
  },
  {
    "reference": "MEKYNAIKIVKGSGGFGGPLTVKPEEGKDTLLYITGGGAEPEIVEKIVNLTGCKAVNGFKTSVPEEQIFLVIIDCGGTLRCGIYPQKRIPTINVMPVGKSGPLAKFITEDIYVSAVGLNQISLADSSAEPIKSTKVPEEGKREFKYSADKKVSQSLAENSKSSIVQKIGMGAGKVVNTLYQAGRDAVQSMITTILPFMAFVAMLIGIIQGSGFGNWFAKILVPLAGNGIGLMILGFICSIPLLSALLGPGAVIAQIVGTLIGVEIGKGTIPPSLALPALFAINTQCACDFIPVGLGLAEAEPETVEVGVPSVLYSRFMIGVPRVAVAWVASIGLYQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that The enzyme II complex composed of SrlA, SrlB and SrlE is involved in glucitol/sorbitol transport. The designed protein sequence is ",
    "response#1": "GPGDLRLENYPIPEPGPNEVLLKMHSVGICGSDVHYWQHGRIGDFIVKKPMVLGHEASGTVVKVGSLVKHLKSGDRVAIEPGALREMDEFCKIGRYNLSPSIFFCATPPDDGNLCQFYKHNADFCYKLPDNVTYEEGALIEPLSVGIHACRRAGITLGNKVLVCGAGPIGLVTLIVAKAMGAGQVLVTDLSASRLSKAKEVGADIVLQISKESPKEIASKVEDMLGCKPEATIECTGVESAIQSGIYATRAGGTLVLVGLGSEMTTVPLTHASTREVDIKGVFRYCNTWPMAISMLASKAVNVKPLVTHRFPLEKALEAFETARKGTGLKVMLS",
    "response#2": "RVVTLAVAAFIFNTTEFVPVGLLSDIAQSFHMQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFTVLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIVGQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLTVVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASALVSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVAMALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGTVPAFAALIWSIIIFRRWR",
    "response#3": "DFSTSMVNEHNTKRALHKDTGSLTWSDTLATYAQNYADSYDCSGNLVHSGGPYGENLALGYGTTGSVDAWYNEITSYDYSNPGFSESAGHFTQVVWKGTSEVGCGLKSCGGEWGDYIICSYKAAGNVIV"
  },
  {
    "reference": "MPRTHAIEDYRNFGIMAHIDAGKTTTTERILYYTGKSHKIGEVHEGAATMDWMEQEQERGITITSAATTCFWRDKRLNIIDTPGHVDFTIEVERSLRVLDGAVCVLDGNQGVEPQTETVWRQADKYDVPRVVFVNKMDKIGADFFKCVADIIGRVAGKPVCLQLPIGAESSFKGVIDLIKMKAIVWSGEALGANFSEEEIPADLADQAVEYRTKMIEACVELDDDAMTAYLDGVEPDEETLRKLVRKAVQLRAFHPVLCGSAFKNKGVQPLLDAVVDYLPSPADRGEIKGLDFKTEEEVVRHPTDSDPFSMLAFKIMDDPHVGTITFCRVYSGKVESGANVINSSRDKKERVGRMLLMHANNREDVKEAFAGDIVALAGLKDTRTGDTLCDPKDAVILEKMEFPEPVIEIAVEPKSKADQEKLGIALSKLAAEDPSFRVSTDQESGQTILRGMGELHLDIKVDILRRTYKVDANIGQPQVAYREKLTRRQEIDYTHKKQTGGTGQFARVKFVVEPNEPGAGFAFESKIVGGAVPKEYIPGVEKGLNSVLGAGVLAGFPVVDVKVELIDGAYHDVDSSALAFEIASRAAFREALQKGGSVLLEPVMKVEVVSPEEYTGSVIGDLNSRRGQIQGQDMRGNANVINAMVPLANMFGYVNQLRSFSQGRANFTMQFDHYEEVPRGEADKVIAKYA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must have high specificity towards GTPase activity. The designed protein sequence is ",
    "response#1": "ANIDQAIIVSSAVDPDFTTLLLDRFLVLIESKHIQPIILITKVDLLNEDQLENIKLYKQHYEKIGYRVELVSSKNQDLLPELEPYFDEKVSVFAGQSGVGKSSLINVLDPSLLLETAEISKSLGRGKHTTRHVELMKIGNGLVADTPGFY",
    "response#2": "SNELVRPPIANIDQAIIVSSAVDPDFTTLLLDRFLVLIESKHIQPIILITKVDLLNEDQLENIKLYKQHYEKIGYRVELVSSKNQDLLPELEPYFDEKVSVFAGQSGVGKSSLINVLDPSLLLETAEISKSLGRGKHTTRHVELMKIGNGLVADTPGFSVI",
    "response#3": "EIGNSLKELRQNAVQQYDILAGRYSESVYLQRKKLLVQKVDLSILEVYTSVLQKLIRQSRDLYLKQIESSLTKKEAGIIFYQVLDNAERESLKYFNENTSLISFVDVDDSEARSYDPSPKLRELEEELSNLRTELVNKEQENIKTKIPRKFKSHFKLIVQDELSNIKPSSWEELLDQFRQVSEKLLAKYKSPDSNYDFHLGLSKEKNKELHEQVLIKFWIKFKEILNDFVTETNVLRLLVSKFEDEFRYDEEGLPVVWKNSAEIDVKFAKARNSALDLLPLFSLAHTAEGEILPDYDIAHDEAEAESDNEEDDGFKESHKFAHLLSARDQDAIRNKFKKQTDALYVETKRSVINSKTEVPLYIYALLLVLGWNEFMIILRNPLLITLLLIGLTGLYLGYKTKLLGPIVQVVQAMIGG"
  },
  {
    "reference": "MDQLRQSLLEAPIIEKGEYEYFVHPISDGVPMLEPGLLREIVIKIIRKADLEDVDKIVTPAAMGIHISTAVSLMTDIPLVVIRKREYGLDGEISLSQQTGYSENDMFINDVSEDDRVLVIDDVLSTGGTMCAVLDALKSTGAEVVDTVAVIKKAGPNELDDTDHHVKTLINVTVEDGEVVIVDENGDD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: May catalyze a purine salvage reaction, the substrate is unknown..2. The protein design should be able to enhance the efficiency of purine ribonucleoside salvage. The designed protein sequence is ",
    "response#1": "DRMEYAVCTPFGKPSDIIIEGLIDGVKCALLCRNGRLHDIMPTNINYRANIWAMRKLGCTHILVTHSLSSLREDIMPGDFVVPNDLIDHTTRRAQTFYDGALGSPFGVCHLPMYPAFCERTRQHLLNAAQELDLATHSKATVLTLEGPRYSTLAENNIYRKWGADLLSMTLSPEATLAKEAGILYASIGLVTNIECWCANQPIATTHEIIYVFKNKVEKLQQVLSKAA",
    "response#2": "PELLREIVIRIIRKAELEDVDKIVTPAAMGIHISTAVSLMTDIPLVVIRKRQYGLEGEVSLQQVTGYSESEMYVNDVYEGDKVLVLDDVLSTGGTLAGITGALEEIGAEIVDIVAVIKKVGGENKIDDSDFL",
    "response#3": "REVVVELMQTAELDVDKIVTPEAMGIHLSTALSLQTDIPLVVIRKRAYGLDGEVSLHQQTGYSESEMYINDVEAGDRVLVVDDLLSTGGTLAAICASLSDIGAEIADVVVVLRKVGPSR"
  },
  {
    "reference": "MRIDILSLFPDMFDATLGQSIVGRAQDDGFVDIKVTDFRQYTTDKHRHVDDAPFGGGAGMLLQAQPIFDAMDAIEQETKDTYPKGRVILMDPAGRRFDQDFAMELAQEEHLTFICGHYEGYDERIRQLVTDEASLGDYVLTGGELAAMVMVDATVRFVPGVLGNMSSPMGDSFSNGLLEYPQYTRPADFRGMKVPEVLTSGNHEKIREWRMRESLKRTLERRPDLLKNAKLSREQQIILQDLKLDLDPDAPK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be optimized for tRNA (guanine(37)-N(1))-methyltransferase activity. The designed protein sequence is ",
    "response#1": "NYMKPKELQNAVCQLLESEGCNRRNVDPFHIYLCNLKTDGDYYRELVKRYRERWDKLLLTATEKSHVDLFPKEDIIYLTADSPNVMTTFKHDKIYIIGSFVDKSMQPGASLANAKRLKLATECLPLDKYLQWETGTKNLTLDQMMRILLCVKNTGSWEEALKFVPRRS",
    "response#2": "MGWKGAQAMQFGQPLVFDMVYENYMKPKELQNAVCQLLESEGCNRRNVDPFHIYLCNLKTDGDYYRELVKRYRERWDKLLLTATEKSHVDLFPKEDIIYLTADSPNVMTTFKHDKIYIIGSFVDKSMQPGASLANAKRLKLATECLPLDKYLQWETGTKNLTLDQMMRILLCVKNTGSWEEALKFVPRRKHTC",
    "response#3": "NYMKPKELQNAVCQLLESEGCNRRNVDPFHIYLCNLKTDGDYYRELVKRYRERWDKLLLTATEKSHVDLFPKEDIIYLTADSPNVMTTFKHDKIYIIGSFVDKSMQPGASLANAKRLKLATECLPLDKYLQWETGTKNLTLDQMMRILLCVKNTGSWEEALKFVPRRR"
  },
  {
    "reference": "MTARGTRKNYQRLWRWGTMLLGMLMICSAAENLWVTVYYGVPVWKEATTTLFCASDARAYATEVHNVWATHACVPTDPNPQEVVLGNVTENFDMWKNNMVEQMQEDIISLWDQSLKPCVKLTPLCVTLDCTDVNTTSSSLRNATNTTSSSWETMEKGELKNCSFNTTTSIRDKMQEQYALFYKLDVLPIDKNDTKFRLIHCNTSTITQACPKISFEPIPMHYCTPAGFAILKCNDKKFNGTGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVIIRSSNFTNNAKIIIVQLNKSVEINCTRPNNNTRNRISIGPGRAFHTTKQIIGDIRQAHCNLSRATWEKTLEQIATKLRKQFRNKTIAFDRSSGGDPEIVMHSFNCGGEFFYCNTSQLFNSTWNDTTRANSTEVTITLPCRIKQIVNMWQEVGKAMYAPPISGQIRCSSKITGLLLTRDGGKNTTNGIEIFRPAGGDMRDNWRSELYKYKVVKIEPLGVAPTKARRRVVQREKRAVGMLGAMFLGFLGAAGSTMGARSMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTTVPWNASWSNKSLNEIWDNMTWMQWEREIDNYTHLIYTLIEESQNQQEKNEQELLELDKWAGLWSWFSITNWLWYIRIFIIIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTRLPTQRGPDRPEGIEEEGGERDRDRSGRLVDGFLALIWDDLRSLCLFSYH",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.2. The protein must exhibit the following characteristics: Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state.3. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.4. The protein should have a Helical domain that is long enough to span the entire membrane.5. The designed protein should have structural molecule activity within virion membrane, membrane, viral envelope to regulate positive regulation of establishment of T cell polarity, viral protein processing, actin filament reorganization.6. The protein design should include a YXXL motif; contains endocytosis signal, Di-leucine internalization motif motif to ensure proper function. The designed protein sequence is ",
    "response#1": "MGAAATTLAVQTHTLMKGIVQQQDNLLRAIQAQQQLLRLSVWGIRQLRARLLALETLIQNQQLLNLWGCKGRIICYTSVKWNSTWGNVTSMSEVWDKLTWQEWDQQIDNISNVIFDEIQRAQVQQEQNEKKLLELDEWASIWNWLDITKWLWYIKIAIIIVGALIGVRIVMVVLNLVRNIRQGYQPLSFQTPELWVTVYYGVPVWKEATTTLFCASDAKAYHTEAHNVWATHACVPTDPNPQEINLENVTENFNMWKNNMVEQMHQDIISLWDESLKPCVKLTPLCVTLKCTDFNGNGTNTSSTQPTTTSSTSNSNISLEMEVGEIKNCSFNVTRGGDKYQKEYALFYKLDLVTTNDKDNTSYTLINCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNEKNFTGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVVLRSANFSDNTKTIIVQLTKHVNITCMRPNNNTRRGIHIGPGRAFYAAGDIIGNIRQAHCNLSRVEWNNTLKQIAIKLKEQFNKTIVFNQSSGGDPEIVMHSFNCGGEFFYCNTTQLFNSRWNMTANWTMNVTTGTDNITLPCRIKQIINRWQEVGKAMYAPPIAGQIKCLSNITGLLLTRDGSNQTNGTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQRERRAALGMMFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLALERYLRDQQLLGIWGCSGKLICTTNVPWNSSWSNKSQEEIWQSMTWLQWDKEISNYTDTIYRLIEESQNQQEKNEQDLLAFDQWANLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSIIKRVRQGYSPLSL",
    "response#2": "MKQCAFNITTVLKDKKEKKQALFYVSDLHKFYDNETNSTMYRLTNCNSSTIRQACPKVSFQPIPIHYCAPAGYAIFKCNSSEFNGTGTCRNITVVTCTHGIKPTVSTQLILNGTLSEGKIRIMGKNISDSGKSILVTLNSTINITCERPWNLSLQEIRIGPMAWYSIALQTQPQARLAHCTYNTTDWEKALKQTAERYLELVNYTSGNVNITFNRSNXGGDAEITQLHFNCHGEFFYCNTSSLFNYSFSCNGTNCTINQTKNETWIPCRIKQVVRSWIQGGSGLYAPPIRGALKCESNITGIILEMDQPWNKSSPDVTFRPIGGDMKDIWRTELFRYKVVKVKPFSVAPTKIARPVIGTNTHREKRXLGPGAMFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQRMLQLTVWGIKQLQARVLAVERYLKDQQFLGLWGCSGKIICTTAVPWNSTWSNKTYEEIWNNMTWIEWEREISNYTNQIYEIITESQNQQDRNEKDLLELDKWASLWNWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSLVKRVRQGYSPLSFQTLPHHQREPDRPERIEEEGGEQGRDRSIRLVSGFLALAWDDLRSLCLFSYHQLRDFILVTAR",
    "response#3": "MKQCAFNITTVLKDKKEKKQALFYVSDLHKFYDNETNSTMYRLTNCNSSTIRQACPKVSFQPIPIHYCAPAGYAIFKCNSSEFNGTGTCRNITVVTCTHGIKPTVSTQLILNGTLSEGKIRIMGKNISDSGKSILVTLNSTINITCERPWNLSLQEIRIGPMAWYSIALQTQPQARLAHCTYNTTDWEKALKQTAERYLELVNYTSGNVNITFNRSNXGGDAEITQLHFNCHGEFFYCNTSSLFNYSFSCNGTNCTINQTKNETWIPCRIKQVVRSWIQGGSGLYAPPIRGALKCESNITGIILEMDQPWNKSSPDVTFRPIGGDMKDIWRTELFRYKVVKVKPFSVAPTKIARPVIGTNTHREKRXRLGXVXMXLWVTVYYGVPVWKEATTTLFCASDAKAYKTEAHNVWATHACVPTDPNPQEVDLVNVTENFNMWKNDMVEQMHEDIISLWDESLKPCVKLTPLCVTLKCMNINCTMKNNTATATQCSSVNTNMSYSYNRSGLETDMRNCSFKVTTAVKDRVKQEYALFYRLDIEPIEGEQDNTSYRLINCNTSAQTQACPKVSFEPIPIHYCAPAGFAILKCKDKNFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEDIVLRSENISDNSKNILVLLNSSVDITCIRPSNNTRKSISIGPGRAFYATGDIIGDIRQAHCNXSKANWTNILKQIAIKLKEHFNKTIAFKNSSGGDPEIVMHTFNCGGEFFYCNSTPLFNWTLNDTKKENDTEEDKNITLQCRIKQFINMWQGVGRAMYAPPIQGQIKCSSNITGLILTRDGGKSDNPSEIFRPGGGNMKDNWRSELYKYKVVKIEPLGVAPTKARRRVVQREK"
  },
  {
    "reference": "MKVFEADSLLSEADKRTKEYKELRSQMVKLKKAFKAVADLDDSKFSGKGADNIKAFYHDHVGVTDQWIDLIDMKIVFLSSISAKLEDAKMSDAYIEESFLEHELVNAYTKSKSIMSEQKKAMKDILNDINDILPLEIFSTEDFKDKLSSADDKREKTIDKINKLDEDLKTEYAETEQNEQFIQQDFKKLQESTGKGKNATPIHYSAKAYRESDIHKKKGDIEQHSEAYLTVKKEEAKEREIKELKKKLNDGVSDPDEYLEIAKKVGYENLEPAQVQLAVQIEQAKQLEGAGEITWDIVKGVGVGLYDVGKDTVTGLWDFITDPGETLSALGNAVIHPVKTYDAISAAIEESYQKDMVNGDAYSRSRWVTYAIGSVAAAVIGTKGAGAINKADAAGKVINKASQAGKKIKDVKIPDLLPYNPKYDLAMAGDVPYNVVDGENLKNQLMSFAKGSDKEVKPFDVVDYRPSNSPLENHHGVMDVWAKHNVPNYVSRGSNTPTVALTKEQHNATKKVYREWLFEKTGKKVGGKVNWKEVSPREIQELTEKMFDAANVPKEARQQYYNAFNQYNFRK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Co- overexpression with cognate immunity protein YokJ does not cause growth arrest; Toxic component of one of 6 LXG toxin-immunity modules in this strain; No effect is seen on rRNA; They promote kin selection, mediate competition in biofilms, and drive spatial segregation of different strains, indicating that LXG toxins may help avoid warfare between strains in biofilms; Overexpression alone in situ causes growth arrest but not cell lysis, a large decrease in chromosomal DNA content and the production of anucleate cells.2. The protein must exhibit nuclease activity, toxin activity.3. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "PNIIYKTKEGYTYTTDNYGRITSVKADLQLGEAKRNQYAQTNAGKPQDRKPDDDGGHLIATQFKGSGQFDNIVPMNSQINRSGGKWYEMEQEWAKALSKKPPKKVAVQIEPVYSGDSLRPSYFDVTYKII",
    "response#2": "NSSIDILSVSDQPQEGITSYSTLGLSDHSINYEVNGTPLRIEIVAAMESASDIYANVLSTCAFNIINSNFTCAPGVIFKNVISMYDQETDMKHIMFVPPFLWEEDLELLEFSNKNVTWLMALPISEGELQVAEKHGSDYLQDLLESKQIDIFDIKRR",
    "response#3": "NSSIDILSVSDQPQEGITSYSTLGLSDHSINYEVNGTPLRIEIVAAMESASDIYANVLSTCAFNIINSNFTCAPGVIFKNVISMYDQETDMKHIMFVPPFLWEEDLELLEFSNKNVTWLMALPISEGELQVAEKHGSDYLQDLLESKQIDIFDIKRR"
  },
  {
    "reference": "MKSYLVGGAVRDALLGLPVKDRDWVVVGATPQQMLDAGYQQVGRDFPVFLHPQSREEYALARTERKSGAGYTGFTCYAAPDVTLEADLLRRDLTVNALAQDADGAIIDPYGGQNDLRKRLLRHVSPAFSEDPLRVLRVARFAARYAHLGFRIAEETQALMAAIVEAGELAHLTPERVWKETESALTTRNPQVFFQTLRDCQALKVLFPEIDALYGVPAPAKWHPEIDTGLHTLMTVTMAAMLSPDVDVRFATLCHDLGKGLTPKALWPRHHGHGPAGVKLVEQLCARLRVPNDIRDLAKLVAEYHDLIHTLPILQPKTLVKLFDSIDAWRKPQRVQQIALTSEADVRGRTGFEASDYPQGRLLLEAWEVAQSVSTKEVVAAGFKGAEIREELTRRRIAAVAQWKEQRCPQPQG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should contain one or more HD domains that are essential for its function.2. The designed protein must be able to perform cyclic-nucleotide phosphodiesterase activity under specific conditions.3. For general function, the protein need meet that Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate The designed protein sequence is ",
    "response#1": "TGIHTLLVAEQAAKLSSSAVVRFAAQVHDLGKGVTPPSEWPSHKMHCHTGLKLIKQLCERVRVPNEFRDLALMVCEQHSNIHRAAELKPQTMVKIFNKLDVL",
    "response#2": "TGVHVMMVVDHAAAQNYALAVRFAALTHDLGKATTPEDVLPRHIGHEGRSVDLLKPLCERLRVPNECRDLALLVAREHGNIHRVMETKAAGLVRLFERSDAG",
    "response#3": "DTGVHLMMVLDMAAHLQAPLTVRFACLTHDLGKGTTPHDVLPRHIGHEQRSARLLKAVCERLRVPVECRELADVVAREHGNIHRSGDLGAAALVRLLERCDAIRKPARLDEILLACECDARGRLGFADRPL"
  },
  {
    "reference": "MIFTLLSTLPVLIITTELDYSELVHSAELVSSSSYIHHKTNKKPDNCTRDTDIIDRLLNGTGYNKFRIPQEEGMTVVVEIWIQAITSIDELTNDFDMDIYITETWLDPALNFQTMTPCKGNLSLNHQVLDRLWTPNSCFINSKVAQIHNSPFRSVFLMLFPNGTVMVNYRVRVKGPCSLDLSNFPLDLQKCSLIYESFNYNRQEVEMRWSDAEHPVFNLSKIMLPDFDLFEIQTERRQEPYPAGMWDELHVTIIFERRFIWYFMQAYLPTYLTIFISWISFSLGSRAIPARTMLGVNSLLAIVFSFGNIMRNLPRVSYIKGIDVWMLVSMTFIFCSLLELAIVGFMVRDETVAKKKQQKKISGNISREESPHGIISERRFMFPPGCSESSKSLSSCTSGWTPERIDSISSVMFPFSFFVFNIIYWFYYIHRKEIIKQNLINRVDG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have excitatory extracellular ligand-gated ion channel activity, transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential, chloride channel activity, transmembrane signaling receptor activity, neurotransmitter receptor activity within chloride channel complex, postsynapse, plasma membrane, synapse to regulate chloride transmembrane transport, regulation of membrane potential, signal transduction, nervous system process.2. For general function, the protein need meet that Currents in channels are triggered in response to acetylcholine; Acetylcholine-gated chloride channel subunit; Channel properties may be modulated by the formation of homomeric and heteromeric channels.3. The designed protein should contain Extracellular topological domains.4. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "ILNSLLRDYDSKLRPDIGVRPTVIETDVYVNSIGPVDPINMEYSIDIIFGQTWFDRRLRFPGSSKVLMLNSNVVGKIWVPDTFFRNARKSDAHWVTTPNRLLRIRGDGRVLYTLRLTIAAECYLQLRNFPMDEHSCPLEFSSYGYPQNEIEYKWKQTSVEVADPKYWRLNQFAFVGLRNSTEIWRTQSGDYVVMTIFFDLSRRR",
    "response#2": "ESQLYEDLLFDYNKVPRPVKNSSDILTVDVGASLIRIIDVDEKNQVLTTNLWLEMKWNDAKLTWTPEKYGGLKTLHIPSDFIWTPDLVLYNNAAGDPDITILTDALVTFEGNVYWQPPAIYKSFCPIDVTWFPYDSQKCEMKFGTWTYTGRYVDLKQLPQEEVVTITKDDNDVEFMQQGMDLSFFYRSAEWDLLSLTSERHSVLYASCCGPEKYVDITYYFGLRRKK",
    "response#3": "ILNSLLRDYDSKLRPDIGVRPTVIETDVYVNSIGPVDPINMEYSIDIIFGQTWFDRRLRFPGSSKVLMLNSNVVGKIWVPDTFFRNARKSDAHWVTTPNRLLRIRGDGRVLYTLRLTIAAECYLQLRNFPMDEHSCPLEFSSYGYPQNEIEYKWKQTSVEVADPKYWRLNQFAFVGLRNSTEIWRTQSGDYVVMTIFFDLSRRR"
  },
  {
    "reference": "MLFRFGVVVPPGVAQGRPHLLVVGSRPELGRWEPQKAVPMKRAGGAAGPDGARGLQEPGLWLAEVELAGEPPPEGAGPGVPDTFWYKFLKREPGGEFSWEGNGPHHDRCSTYNENNLVDGVYCLPIGHWIEATGHTNEMKHTTDFYFNIAGHQAMHYSRILPNIWLGSCPRQLEHVTIKLKHELGVTAVMNFQTEWDIIQNSSGCNRYPEPMTPETMIKLYKEEGMVYVWMPTPDMSTEGRVQMLPQAVCLLHSLLENGHTVYVHCNAGVGRSTAAVCGWLKYVMGWNLRKVQYFLLSKRPAVYIDEEALARAEEDFYQKFGKVRSSLCNL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit protein tyrosine/serine/threonine phosphatase activity. The designed protein sequence is ",
    "response#1": "EIAEVVASSLPSPLRREKLAVALENEGYLKKLLELFHVCEDLENIEGLHHLYEVIKGIFLLNRTALFEVMFSEDCMMDIIGCLEYDPSLPTQRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDVVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTELFAHLTDEATDDEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDPQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDDILLINLIIEHMICDTDPELGGAVQLTGLLRTLVDPENMLATTNKTEKTEFLGFFYKHCMHVLTAPLLANTTEEKPSKDDFQAAQLLALILELLTFCVEHHTYHIKNYIINKDILRRVLVLMSSMHSFLGLCALRFMRKIVGLKDEFYNRYIVKSFLFEPVVKAFLNNGSRYNLMNSAILEIFEYIRVEDIKSLTAHVVENYWKALEDVEYVQTFKGLKLRYDC",
    "response#2": "EIAEVVASSLPSPLRREKLAVALENEGYLKKLLELFHVCEDLENIEGLHHLYEVIKGIFLLNRTALFEVMFSEDCMMDIIGCLEYDPSLPTQRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDVVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTELFAHLTDEATDDEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDPQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDDILLINLIIEHMICDTDPELGGAVQLTGLLRTLVDPENMLATTNKTEKTEFLGFFYKHCMHVLTAPLLANTTEEKPSKDDFQAAQLLALILELLTFCVEHHTYHIKNYIINKDILRRVLVLMSSMHSFLGLCALRFMRKIVGLKDEFYNRYIVKSFLFEPVVKAFLNNGSRYNLMNSAILEIFEYIRVEDIKSLTAHVVENYWKALEDVEYVQTFKGLKLRYDC",
    "response#3": "EIADLVTSVLSSPIRREKLALALENEGYIKKLLQLFQTCENLDNTEGLHHLYEIIRGILFLNKAALFEVMFSDECIMDVVGCLEYDPALAQPKRHREFLTKTAKFKEVIPITDSELRQKIHQTYRVQYIQDVILPTPSVFEENFLSTLTSFIFFNKVEIVSMLQEDEKFLSEVFAQLTDEATDDDKRRELVNFFKEFCAFSQTLQPQNRDAFFKTLANLGILPALEIVMGMDDLQVRAAATDIFSYLVEFSPSMVREFVMQEAQQSDDDILLINVVIEQMICDSDPELGGAVQLMGLLRTLIDPENMLATANKTEKSEFLNFFYNHCMHVLTAPLLANTSEDKLEKDAVLGSIKTSTVCPDNYQTAQLLALILELLTFCVEHHTYHIKNYIMNKDLLRRVLILMNSKHTFLALCALRFMRRIIGLKDEFYNRYIIKGNLFEPVINALLDNGTRYNLLNSAIIELFEFIRVEDIKSLTSHIVENFYKALESIEYVQTFKGLKTRYEC"
  },
  {
    "reference": "MAVWLAQWLGPLLLVSLWGLLAPASLLRRLGEHIQQFQESSAQGLGLSLGPGAAALPKVGWLEQLLDPFNVSDRRSFLQRYWVNDQHWVGQDGPIFLHLGGEGSLGPGSVMRGHPAALAPAWGALVISLEHRFYGLSIPAGGLEMAQLRFLSSRLALADVVSARLALSRLFNISSSSPWICFGGSYAGSLAAWARLKFPHLIFASVASSAPVRAVLDFSEYNDVVSRSLMSTAIGGSLECRAAVSVAFAEVERRLRSGGAAQAALRTELSACGPLGRAENQAELLGALQALVGGVVQYDGQTGAPLSVRQLCGLLLGGGGNRSHSTPYCGLRRAVQIVLHSLGQKCLSFSRAETVAQLRSTEPQLSGVGDRQWLYQTCTEFGFYVTCENPRCPFSQLPALPSQLDLCEQVFGLSALSVAQAVAQTNSYYGGQTPGANKVLFVNGDTDPWHVLSVTQALGSSESTLLIRTGSHCLDMAPERPSDSPSLRLGRQNIFQQLQTWLKLAKESQIKGEV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Incorporate a signal peptide in the protein design.2. For general function, the protein need meet that Protease that may play a role in T-cell development.3. The Charge relay system active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.4. The ideal protein would be one that plays a critical role in proteolysis, protein catabolic process through dipeptidyl-peptidase activity, serine-type peptidase activity within endosome, lysosome. The designed protein sequence is ",
    "response#1": "IVGGTNAPRGKYPYQVSLRAPKHFCGGSISKRYVLTAAHCLVGKSEHQVTVGSVLLNKEEAVYNAKELIVNKNYNSIRLINDIGLIRVSKDISFTQLVQPVKLPVSNTIKAGDPVVLTGWGRIYVNGPIPNNLQQITLSIVNQQTCKSKHWGLTDSQICTFTKRGEGACHGDSGGPLVANGVQIGIVSYGHPCAIGSPNVFTRVYSFLDWIQKNQC",
    "response#2": "IVGGTNAPRGKYPYQVSLRAPKHFCGGSISKRYVLTAAHCLVGKSEHQVTVGSVLLNKEEAVYNAKELIVNKNYNSIRLINDIGLIRVSKDISFTQLVQPVKLPVSNTIKAGDPVVLTGWGRIYVNGPIPNNLQQITLSIVNQQTCKSKHWGLTDSQICTFTKRGEGACHGDSGGPLVANGVQIGIVSYGHPCAIGSPNVFTRVYSFLDWIRSHT",
    "response#3": "IVGGTNAPRGKYPYQVSLRAPKHFCGGSISKRYVLTAAHCLVGKSEHQVTVGSVLLNKEEAVYNAKELIVNKNYNSIRLINDIGLIRVSKDISFTQLVQPVKLPVSNTIKAGDPVVLTGWGRIYVNGPIPNNLQQITLSIVNQQTCKSKHWGLTDSQICTFTKRGEGACHGDSGGPLVANGVQIGIVSYGHPCAIGSPNVFTRVYSFLDWIQKNQC"
  },
  {
    "reference": "MGGLTISDLVVEYSSGGYAVRPIDGLSLDVAPGSLVILLGPSGCGKTTLLSCLGGILRPKSGSIKFDDVDITTLEGAALAKYRRDKVGIVFQAFNLVSSLTALENVMVPLRAAGVSRAAARKRAEDLLIRVNLGERMKHRPGDMSGGQQQRVAVARAIALDPQLILADEPTAHLDFIQVEEVLRLIRSLAQGDRVVVVATHDSRMLPLADRVLELMPAQVSPNQPPETVHVKAGEVLFEQSTMGDLIYVVSEGEFEIVRELADGGEELVKTAAPGDYFGEIGVLFHLPRSATVRARSDATAVGYTAQAFRERLGVTRVADLIEHRELASE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a binding site that is specific to the target ATP, a nucleoside 3',5'-cyclic phosphate ligand.2. A protein that contributes to transmembrane transport by functioning in plasma membrane with ATP hydrolysis activity, ATP binding, transmembrane transporter activity.3. The presence of ABC transporter domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "LNGLNLTLKPGQVVALAGSSGGGKSTIAGLLERFYDISNGDITIDGYSIKQLNAKWLRSRIGIVSQEPSLFATTILENLRYGNPNATEDEIIEAAKLANAHQFISNFPKGYETIVGERGVQLSGGQKQRIAIARAILKNPQIIILDEATV",
    "response#2": "PGQVVALAGSSGGGKSTIAGLLERFYDISNGDITIDGYSIKQLNAKWLRSRIGIVSQEPSLFATTILENLRYGNPNATEDEIIEAAKLANAHQFISNFPKGYETIVGERGVQLSGGQKQRIAIARAILKNPQIIILDEATSALDSQSELLVQTALDNLMKGRTTLVIAHRLSV",
    "response#3": "LNGLNLTLKPGQVVALAGSSGGGKSTIAGLLERFYDISNGDITIDGYSIKQLNAKWLRSRIGIVSQEPSLFATTILENLRYGNPNATEDEIIEAAKLANAHQFISNFPKGYETIVGERGVQLSGGQKQRIAIARAILKNPQIIILDEATR"
  },
  {
    "reference": "MASAASSLPLLVSSLLLALFALGAHADVKRYQFDIVMSNVSRLCHEKAMVTVNGSYPGPTIYAREGDRVIVNVTNHVKHNMTIHWHGLKQRRNGWADGPAYVTQCPIGSGGSYVYDFNVTRQRGTLWWHAHIAWMRATVHGAIVILPAAGVPYPFPKPDDEAEIVLGEWWHADVETVERQGSMLGMAPNMSDAHTINGKPGPLVPFCSEKHTYALQVQSGKTYLLRIINAAVNDELFFSIAGHNMTVVEIDATYTKPFAASTVQLSPGQTMNVLVSADQSPGRYFMVAKPFNDVPIPADNKTATAILQYAGVPTSVVPALPQTMPATNSTGSVAAFHDKLRSLNSPRYPADVPLAVDRHLLYTIGLNIDPCETCLNRSRLAASLNNITFVMPRTALLQAHYYGQKGVFAADFPDRPPARFNYTGVPLTAGLGTSLGTRLSKIAYNATVELVLQDTNLLSVESHPFHLHGYNFFVVGRGVGNFDPAKDPAKYNLVDPPERNTVGVPAGGWTAIRFRADNPGVWFLHCHLEVHTSWGLKMAFLVEDGSGPDESVLPPPKDLPKC",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a binding site that is specific to the target Cu cation ligand.2. For general function, the protein need meet that Lignin degradation and detoxification of lignin-derived products.3. The designed protein have Plastocyanin-like domains with specific sequence motifs that are crucial for its activity.4. The protein must be engineered to have a high affinity for Cu cation in order to increase its catalytic activity.5. The designed protein should have copper ion binding, oxidoreductase activity, hydroquinone:oxygen oxidoreductase activity within apoplast to regulate lignin catabolic process.6. Incorporate a signal peptide in the protein design.7. We are looking for a protein with broad substrate specificity for 4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O. The designed protein sequence is ",
    "response#1": "LLMTDHLHSSAFEEYHKELEGKPPAMDSIILNGKGDYDQTGDLKKKYRTVLKPGKKYLLRLINTSVATTFVFSIDGHKFQVVGSDFVPIEPYVTDHIAVGIGQRYHVILEGLSEEEAKKNGRYWVRTTPGGVWIMHCHLDAHISWGLAMAGGVWLLHCHIERHATWGMNTVGGTSIYTDDFPNQPPLKFDYTKFEQRTNNDMKMMFPERKTSVKKIRFNSTVEIVLQNTAIISPESHPMHLHGFNFYVLGYGFGNYDPIRDARKLNLFNPQMHNTVGVPPGGWVVLRFIANNPGVWLFHCHMDAHLPYGIMSAFIVQNG",
    "response#2": "ESTVITLADWYHEAASLIVPPADPDSVLINGRGRQANDTTSPLAVINVEYGKRYRIRLISVSCDPYFNFTIDGHNFTIIEADGENTDPLPGVDQIQIFAAQRYSFILDANQPIDNYWIRVIPEQVGANGTAATPPGLAVLHYLQRDPNGPNESTVITLADWYHTPAPLLPPAATLINGLGRWPGNPTADLAVIEVQHGKRYRFRLVSTSCDPNYNFTIDGHTMTIIEADGQNTQPHQVDGLQIFAAQRYSFVLNANQAVNNYWIRANPNRANTTGFANGINSAILRYKGDGSWLLHCHIAWHASAGLALQNVYTADFPASPLEPFNYTGTPPNNTNVSNGTRVVVLPYNASVEVVLQDTSILGAESHPLHLHGFDFFVVGQGTGNYDPSKHPAEFNLVDPVQRNTVGVPAGGWVAIRFFADNPGVWFMHCHLEVHTTWGLKMAWVVNDDFPNQPPLKFDYTKFEQRTNNDMKMMFPERKTSVKKIRFNSTVEIVLQNTAIISPESHPMHLHGFNFYVLGYGFGNYDPIRDARKLNLFNPQMHNTVGVPPGGWVVLRFIANNPGVWLFHCHMDAHLPYGIMSAFIVQQL",
    "response#3": "VYTRDFPDTPPVVFDYTGDESDNATMQFTTKSTKVKTLRYNETVEMVLQNTRLIAKESHPMHIHGFNFFILAQGFGNYDKRRAERRFNLVDPQERNTIAVPTGGWAVIRFVADNPGMWYMHCHFDAHISLGLAMVLEVLDEVPIVFQQWWDTDVRLLQLRPAPVSDAYLINGLAGDSYPCSENRMFNLKVVQGKTYLLRIVNAALNTHLFFKIANHNVTVVAVDAVYSTPYLTDVMILTPGQTVDALLTADQAIGKYYMATLPYISAIGIPTPDIKPTRGLIVYQGGGVWFMHCHLEVHTTWGLKMAGVPPTVPVLLQILSGAQDAASLLPNGSVYTLPSNSTIEISFPIITTDGVLNAPGAPHPFHLHGHTFSVVRSAGSSTFNYANPVRRDTVSTGNSGDNVTIRFTTDNPGPWFLHCHIDFHLEAGFAIVWGER"
  },
  {
    "reference": "MSTRVELFCDGACSGNPGVGGWGGILRCGATEKELSGAEWETTNNRMEMTAAIRGLEALTRPCEVVVTTDSQYLVKGMTEWLSGWIRRGWVNSKKEPVLNRDLWERLLELSKTHRIQWTWIRGHNGHAENERCDALARGAIDELRKKGQGRR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.2. The protein should have a low Km value for Endonucleolytic cleavage to 5'-phosphomonoester..3. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.4. The protein contains novel RNase H type-1 domains that confer a unique function or activity.5. The protein must exhibit the following characteristics: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids..6. The designed protein should have nucleic acid binding, magnesium ion binding, RNA-DNA hybrid ribonuclease activity within cytoplasm to regulate RNA catabolic process. The designed protein sequence is ",
    "response#1": "MVEIFSDGACSGNPGPGGFGTLLRCGERVRELSGFDPETTNNRMELLGAIAGLEALTRPCRVRLTTDSQYVCKGMTEWIHGWQKKGWKNSKKEDVANRDLWERLLVLVSKHEVSWHWVRGHAGHAENERCDELARQAIDAAR",
    "response#2": "MKVTIYTDGAARGNPDGPGGYGTILSYIDSTGVEHIREYSGGYKKTTNNRMELMAAIVGLEALTKPCVVTLYSDSQYVVKAFNEHWLDGWIKKGWKRGKNEPVKNVDLWKRLLAAKNQHDVTFCWVKGHDGHPQNERCDVLATTAARAA",
    "response#3": "MVEIFSDGACSGNPGPGGFGTLLRCGERVRELSGFDPETTNNRMELLGAIAGLEALTRPCRVRLTTDSQYVCKGMTEWIHGWQKKGWKNSKKEDVANRDLWERLLVLVSKHEVSWHWVRGHAGHAENERCDELARQALARL"
  },
  {
    "reference": "MDTCVFPLVVLWISMRITSTLDEVPIGGIFDSRSVQALTAFRHEIHMFNRAYSHVYRYKLKNDTTILDVTDSFAVSNALCHHLSRGDLAIFGVSNASSLATIQSYTDTFNVPFVTISMAQNNSHNGSYQIYMRPMYINALVDVIVHYRWEKVAFYYDSDEGLVRLQQLFQATNKYDKMIISIDTKRITSVENGYHMLKELHLMDPEMEHRVLLDVRTDKAEQIILKVMNDSKINNAKFHFLLGDLGMLEINTTHFKIGGVNITGFQLVDPFNSTSELFISTWSSLDPVYWPGAGTNHVNYEAALAADSVRLFKSAFGSILQKDPNFLRRSRSGTAGKSMKCTDDSEIKTGHGQMILEEMKKVKFEGVTGHVAFNEQGHRKDFTLGVYNVAMTRGTAKIGYWNEREGKLHAHNPRLFQNNSSDMNRTRIVTTIIKEPYVMVNNVIRDGKPLVGNEPVEGFCIDLTKAVAEKVGFDFVIQFVKDGSYGSVLSNGTWDGIVGELIRHEADMAIAPFTITADRSRVIDFTKPFMSLGISIMIKRPQPAGKHFFSFMEPLSSEIWMCIVFAYIGVSVVLFLVSRFSPNEWHLSEAHHSYIANDFSISNSLWFSLGAFMQQGCDISPRSMSGRIVGSVWWFFTLIIISSYTANLAAFLTVERMLTPIDSAEDLARQTEIQYGTIMSGSTKAFFKNSQFQTYQRMWAYMTSAQPSVFVKTHEEGIQRVRQSNGKYAYLTESSTIDYVSNRKPCDTLKVGSNLNSDGFGIGTPVGS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: The postsynaptic actions of Glu are mediated by a variety of receptors..2. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "LSMLTGLAWMADAMEMMILSILAPQLHCEWRLPSWQVALLTSVVFIGMMASSSLWGNVSDQYGRRTGLKISVIWTLYYGILSAFAPVYSWILVLRGLVGFGIGGVPQSVTLYAEFLPMKSRAKCILLIEIFWALGTVFEVLLAIFVMPTLGWRWLLILSALPLMLFAILCFWLPESARYEVLSGNQEKALATLKRIATENGAPMPLGKLIVSRQEDRGKIRDLFSPQFRCTTLLLWFIWFSNAFSYYGLVLLTTELFQAGDVCSISNQRKAVKPKCSLACEYLTVEDYTDLLWTTLSEFPGLLVTLWIIDRVGRKKTMAICFIIFSFSALLLFLCVGRNVLTVFLFIARAFISGGFQAAYVYTPEVYPTATRALGLGTCSGMARVGALITPFIAQVMLESSIYLTVLVYSGCCVLAAVASCFLPIETKGRTLEEIQ",
    "response#2": "FRVGKKIGCGNFGELRLGKNLYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLGSAGEGLPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFTLKTVLMIAIQLLSRMEYVHSKNLIYRDVKPENFLIGRQGNKKEHVIHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRNTPIEALCENFPEEMATYLRYVRRLDFFEKPDYEYLRTLFTDLFEKKGYTFDYAYDWF",
    "response#3": "DIDVGQAHRNESFYQCCSGFCIDLLEKFAEELGFTYELVRVEDGKWGTLENGKWNGLIADLVNRKTDMVLTSLMINTEREAVVDFSEPFMETGIAIVVAKRTGIISPTAFLEPFDTASWMLVGIVAIQAATFMIFLFEWLSPSGYDMKLYLQNTNVTPYRFSLFRTYWLVK"
  },
  {
    "reference": "MCKQRTIKHTISISGKGLHTGAHVTMTFKPAPVNHGYVFQRTDVEGRPKIPALVDQVIDTSRGTTLAHQGVKVHTVEHTLVALAGAEIDNVLIELDGPEPPAMDGSAKFFTQALMDAGIQEQDAFREVYAIREAISYEEPGRKVSLSVLPAEQFQAQVFIDFDSNGFKSQSASLEQLQAFSREFADSRTFCFLHEIEPLLQAGLIKGGSLNSAVVIADRQLSGLEIQRLQKLFKKTDIQIRDGGVLNQEGFRYPNEPARHKLLDLIGDLALIGVPIRGKVVASRPGHKANVELARKIRSVIKQERIRKKFQQVDKQGIIFDINAIQQILPHRYPFLLVDKIIEFSERKIVGIKNVTINEPFFQGHFPGNPIMPGVLQLESMAQVGGILLLNTIENPQTVWVYIAAIDKARFKRPVVPGDTIRFELELVNLRKSICEMKGKATVDGQLVCSANMLASIIPKDKL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that can perform 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity, metal ion binding in cytoplasm to support lipid A biosynthetic process.2. The protein should have a fast turnover rate for a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine +   H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate.3. The protein must exhibit the following characteristics: Involved in unsaturated fatty acids biosynthesis.4. A protein that is capable of catalyzing reactions within the lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 2/6, lipid IV(A) biosynthesis pathway with high efficiency and specificity.5. The protein must bind to Zn(2+) in order to perform its enzymatic function.6. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MAWESETYIQSLLEQKKAIVVTGLPGAGKTTAIRQLLGQGVDFCDIAKERGIKGVTKPDAQPLLDAIRQKTLQELERATQEEGGIYIDSPLGAHVLEKFFRENPEYREDFHIILLETPFAECLKRNQRRDAEYDVQKALDDWHVYNRTRSIMEASGLKWIVVEDSPTPENVVKQICETVSLPVGGETDYRYDPESGRIIIHLSGDMEGYVDKMAHRLADSHRGFRMEKELNEHITVVCRGVEETMIEDVIRELKSGWSC",
    "response#2": "MNAMSLQNRSDGRAPLTVKDLAAALQCELVGNEDVEVTGVCNDSRRAGPGDLFVAVRGAQFDAHKFVPQVIAAGAVGVVSELEHVQGDAWITVPDVLARVGQLAAGWHGNPSRALKV",
    "response#3": "MERDLYSSEALAADPLRTVREDFLFPRGVVYLDGNSLGALSKRAEAELVQTLEQGWGHGLIRSWNDAGWWDKPAKVGDRIAPLVGAAPGQIVVGDGTSVNVFKAVVAAVRMQGTDRTRILTDELDFPSDIYMAEGALELLGFEVRRLPCSELDGAIDEEVAVALLSHVNYRTGAMHDLPSITARVHAAGALAVWDLCHSAGALPVGLDEHGVDLAVGCTYKYLSGGPGSPAFLYVAERHQRVFRQPIQGWMGHVDPFAFEPEYRPAPGIERARCGTPSILSMLALEAALDVWDGVTVERVRAKSLALTDFFLECVQARVPDFEVVTPREHARRGSQVSLRHPEAYGIMQALIARGVIGDFRAPDVMRFGFTPLYTSFADVERAARVLGQVLDEGRYASPEFAVRSTVT"
  },
  {
    "reference": "MKIPAMMSLLLVSVGLRDGVQVQSYSISQLDIFSQETPLDMAPASFDDQYAGCLADMTAALPDLNHSEFQANKVYADGWAQANNQWQERRAWGSVWGSLPPSPPGFRDEHGVALLAYTANSPLHKEFNAAVREAGRSRAHYLHHFSFKTLHFLLTEALQLLRSHRSRGCQQVYRGVHGLRFRPAGPGATVRLGGFASASLKNVAAQQFGEDTFFGIWTCLGAPIRGYSFFPEEEEVLIPPFETFQVINTSRPTQGPARIYLRALGKRSTYNCEYIKEKKCRSGPCWLGSSAPGSISASCSLLLLLLFLVLSALPENPGLQQLTRC",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a high specificity for NAD(+) binding, with minimal off-target effects.2. The designed protein should contain one or more TR mART core domains that are essential for its function.3. Design the protein with a signal peptide incorporated.4. The protein can be expressed in cell surface and perform NAD+ ADP-ribosyltransferase activity without triggering undesirable cellular responses.5. The protein must exhibit the following characteristics: Has ADP-ribosyltransferase activity toward GLP1R.. The designed protein sequence is ",
    "response#1": "PPSLAKYRALRCKIEHVDQNTEEFSRVRKEVLQNNRSEQPVDILQIFRVGRVNEATEFLSKLGNVRLLFHGSPVRNILGILSRGLLLPKVAEDRGVQRTDVGNLGSGIYFSDSLSTSIKYAHAGETDGSRLLVVCDVALGKCVNLFKKDFSLTEAPPGYDSVHGVSETTSVPTDFQDDEFVVYKTNQVKMKYIVKFCTPGDQIKD",
    "response#2": "MTLGIFANCIFCLKVKYLPRQQKKKLQTDIKENGGKFSFLLNPQCTHVIVDSADVLSRCHLNSIQKNDVQIANPAFIQDSVRQRRLLDVRNYDC",
    "response#3": "MTLGIFANCIFCLKVKYLPRQQKKKLQTDIKENGGKFSFLLNPQCTHVIVDSADVLSRCHLNSIQKNDVQIANPAFIQDSVRQRRLLDVRNYDR"
  },
  {
    "reference": "MKKIAIFAKVHDPRCQGVAGELITWLEQRRIVPLVEAHFARHLGRSGVTSEEIPDLADMAVVLGGDGTLISAARLLGGREIPILGVNLGSLGFLTEVTLDELYPALEACLGGDYRVSERMMLAATVERGDDIVFSHRVLNDAVINKGALARIVDMESLVNGHYLTTYKADGLIISTPTGSTGYCLSANGPIVHPDLECLTITPICPHTLTNRPIVLEASAEVTIRLISKNEDVYLTLDGQVGMELKCGDIIRVRRAEHRTRLVMSRSKDYFEVLRTKLKWGER",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that can perform NAD+ kinase activity, metal ion binding, ATP binding in cytoplasm to support NADP biosynthetic process.2. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.3. The presence of a divalent metal cation is necessary for the protein to function in the desired environment.4. The protein must exhibit the following characteristics: Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP..5. The designed protein should be able to compete with endogenous proteins or inhibitors for NAD(+) binding. The designed protein sequence is ",
    "response#1": "MTSSLVIATSNAGKLKELQRILSPALGKLRLVTMSEVGGFDGVVEDGDTFEANARKKARAVAAATGLPALADDSGLCVDALGGRPGVISARYAGEPKSDARNNAKLLAELADVPDERRTARFVCVIALVRHADDPRPLVAEGTWEGRVLREPRGTGGFGYDPLFVVPEAGATAAELDPQAKNAVSHRGRALAALLPALARAL",
    "response#2": "MDLAXAXGXXX",
    "response#3": "MAKLSVVIVAAGQGTRAGGDVPKQWQRLHGRSVIDWSIAAFRAAPRVDQVVLVHHADDLAPLKLPAGCTRVEGGATRAASVRAGLEALADAAPDLVAVHDAARPFVDAGLIDRVIEALADHPGAIPALPVADTLKREGDTVVGTLDRAALWRAQTPQGFHFAAIKAAHAAAAGTDFTDDASVAEWAGLEVTIVPGDADNIKITHPDDLARAERRLAGRTEA"
  },
  {
    "reference": "MPDRHHCLALDADDPLRAFRDEFVLPEGVVYLVGNSLGALPRRTAERVRRTVEDEWGKELVGGWNTAGWYDQPLRAGDRIAPLIGVGPGQVVAGNTTSIAIFQALAAALRLRPDRRVIVSEVHNFPTDHYIVQGLAGLLGGYEVRDLAERRFDDAAVVLLSEVDYRTGARQDVPAVTAEAHAAGALIVWDLCHSAGVMEVDVSEADFAVGCTYKYLNAGPGAPAFLYVNPRHHATAENVLSGWFAHAEPFAFEEGFRPAEGIRRFAVSTPHVVSFAALEAALDIWERVPMAQVRAKSMALTSLFVELVGDALELASPADPQARGSQVSFRHPDGYPVMRALIDRGVHGDFRAPDILRFGFAPLYTSYTDVHDAAATLLEVLGEELWRQEKYARRLSVT",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must have at least one Aminotransferase class V domain.2. The designed protein should have pyridoxal phosphate binding, 3-hydroxykynureninase activity to facilitate tryptophan catabolic process, quinolinate biosynthetic process, L-kynurenine catabolic process.3. The designed protein should have a high specificity for pyridoxal 5'-phosphate binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "PRTARAAAEQQFRNWEQLAVEGHFKGETPWMHFHKRLEEASARVVGAKPVEVVVMNNLTTNLHLLLISFYQPTATRYKVLMEGGAFPSDQYALETQVRMRGLDPDDAIVELVPRPGEHTLRTEDILAKIAELGDSLCTILMGGINYYTGQVYDMAAITEAGHAVGAMVGFDLAHAAGNIPLHLHDWDVDFACWCSYKYLNSGPGGVSGAFVHERFHAAPTHPRTARAAAEQQFRNWEQLAVEGHFKGETPWMHFHKRLEEASARVVGAKPVEVVVMNNLTTNLHLLLISFYQPTATRYKVLMEGGAFPSDQYALETQVRMRGLDPDDAIVELVPRPGEHTLRTEDILAKIAELGDSLCTILMGGINYYTGQVYDMAAITEAGHAVGAMVGFDLAHAAGNIPLHLHDWDVDFACWCSYKYLNSGPGGVSGAFVHERFHAAPTHAAPTHAAPTHAAPTH",
    "response#2": "PRTARAAAEQQFRNWEQLAVEGHFKGETPWMHFHKRLEEASARVVGAKPVEVVVMNNLTTNLHLLLISFYQPTATRYKVLMEGGAFPSDQYALETQVRMRGLDPDDAIVELVPRPGEHTLRTEDILAKIAELGDSLCTILMGGINYYTGQVYDMAAITEAGHAVGAMVGFDLAHAAGNIPLHLHDWDVDFACWCSYKYLNSGPGGVSGAFVHERFR",
    "response#3": "PRTARAAAEQQFRNWEQLAVEGHFKGETPWMHFHKRLEEASARVVGAKPVEVVVMNNLTTNLHLLLISFYQPTATRYKVLMEGGAFPSDQYALETQVRMRGLDPDDAIVELVPRPGEHTLRTEDILAKIAELGDSLCTILMGGINYYTGQVYDMAAITEAGHAVGAMVGFDLAHAAGNIPLHLHDWDVDFACWCSYKYLNSGPGGVSGAFVHERFQ"
  },
  {
    "reference": "MPLCSVEEAVADIRQGKMIILVDDENRENEGDLTMAAEHVRPESVNFMAKYGRGLICLPMAPELVDRLQLPLMAQRNSSRFGTNFTVSIEASVGTTTGISAADRAVTIRAAVADNARPEDIVTPGHVFPIRARDDGVLARAGQTEGGVDLARLAGLKPAAVICEVMCDDGSMARMADLEQFSQEHRIKIAAVKDIIRYRLERGQVSVRCEARAHLPSLYGDFTIHAYGSEIESDTHLALVKGDISGPEPVLVRVHSQCLTGDALGSLRCDCRGQLGAALWQIEKERRGALLYMRQEGRGIGLANKIRAYALQDQGYDTVEANRKLGFPDDLRDYGIGAQILVDLGIRKIRLLTNNPKKIVGLSGYGIEIVERVPVEMEACSENEAYLRTKKEKMAHLLSDVYAH",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The Proton acceptor; for GTP cyclohydrolase activity, Nucleophile; for GTP cyclohydrolase activity active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. A protein should be able to manipulate the activity of enzymes in the 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4, Cofactor biosynthesis pathway, either by activating or inhibiting their function.3. The protein should be able to bind GTP, Zn(2+), Mg(2+), D-ribulose 5-phosphate ligand in a variety of conditions4. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.5. The designed protein should have magnesium ion binding, GTP binding, zinc ion binding, manganese ion binding, GTP cyclohydrolase II activity to facilitate riboflavin biosynthetic process. The designed protein sequence is ",
    "response#1": "RAAEAYLPTKFGGDFKAIAYTNEVDAHEHLALVKGEISPEEPVLVRVHSECLTGDVLGSERCDCGEQLKGAMQMIKKEGKGVLVYMHQEGRGIGLANKLKAYSLQDTGLDTVEANERLGFKADLRDYGIGAQILLDIGVKKMRIITNNPKKIVGLEGYGLEVTERVPYLDHRGIAGDRAAEAYLPTKFGGDFKAIAYTNEVDAHEHLALVKGEISPEEPVLVRVHSECLTGDVLGSERCDCGEQLKGAMQMIKKEGKGVLVYMHQEGRGIGLANKLKAYSLQDTGLDTVEANERLGFKADLRDYGIGAQILLDIGVKKMRIITNNPKKIVGLEGYGLEVTERVERVTQAHMPTKYGNFKMVGYVNKVNGEHHIALVKGDITDGEPVLMRVHSECLTGDAFGSLRCDCGDQFAAAMKKINEEGRGLLLYMRQEGRGIGLINKIKAYALQDTGMDTVEANLALGFSEDMRDYGIGAEIIRDLGIKKIKLMTNNPKKINGLEGYGLEIVERVPNVPEGAKATRVLME",
    "response#2": "VASALLPTAFGDFVAVGFRSEIDGIEHIALVRGEIGAGDDVLVRVHSECLTGDVFGSLRCDCGPQLQAALRRIAEEGRGVVLYIRGHEGRGIGLLHKLQAYELQDNGRDTVDANLELGLPADARDYGTGAQILADLGIRSMRLLTNNPTKRAGLEGYGLSIVEPVPTEKHPYVRAAEAYLPTKFGGDFKAIAYTNEVDAHEHLALVKGEISPEEPVLVRVHSECLTGDVLGSERCDCGEQLKGAMQMIKKEGKGVLVYMHQEGRGIGLANKLKAYSLQDTGLDTVEANERLGFKADLRDYGIGAQILLDIGVKKMRIITNNPKKIVGLEGYGLEVTERVREVEVKMPTEFGVFKAVAYTNDVDNKEHVALVKGEIDSSQPTLVRVHSECLTGDVFHSHRCDCGPQLEAALRQIDSEGSGVLLYMRQEGRGIGLINKLKAYKLQEEGLDTVEANQKLGFAPDLRDYGIGAQILKDLGVSQIKLLTNNPKKIRGLEGYGLEVVERVYP",
    "response#3": "VASALLPTAFGDFVAVGFRSEIDGIEHIALVRGEIGAGDDVLVRVHSECLTGDVFGSLRCDCGPQLQAALRRIAEEGRGVVLYIRGHEGRGIGLLHKLQAYELQDNGRDTVDANLELGLPADARDYGTGAQILADLGIRSMRLLTNNPTKRAGLEGYGLSIVEPVPAEHVPVVTDEDRAAEANLPTSHGEFRMIGFENAINGEHHVALVKGDIKPGDEVLVRVHSECLTGDAFGSMKCDCGEQLQAALDKIEKEGKGVLLYMRQEGRGIGLINKIKAYALQDQGMDTVEANLALGFAEDLRDYGIGAQILSDLGVSKVRLMTNNPLKLVGLSGHGLEIVSRREVQVKMPTDFGQFEAIAYSNLVDDKEHVALVKGKIDGNQPVLVRVHSECLTGDVFHSHRCDCGPQLAAALKQIHEAGSGVLLYMRQEGRGIGLLNKLKAYKLQEQGLDTVDANIKLGFAPDLRDYGIGAQILKDLGIRQIRLLTNNPRKIRGLEGYGLEVVERVERVTQAHMPTKYGNFKMVGYVNKVNGEHHIALVKGDITDGEPVLMRVHSECLTGDAFGSLRCDCGDQFAAAMKKINEEGRGLLLYMRQEGRGIGLINKIKAYALQDTGMDTVEANLALGFSEDMRDYGIGAEIIRDLGIKKIKLMTNNPKKINGLEGYGLEIVERVPRTRAGDVLLPTEFGDFKLIPFLDTTTKQEHIALIKGTWEPNEPVLTRVHSSCITGDIFGSMRCDCGPQLHKAMQLIEKEGKGVIVYMNQEGRGIGLFNKIKAYKLQEEGMDTVEANLALGFNEDERDYGVGAQILKKLGVHKMRLLTNNPIKRIGLEGFGIEVVEN"
  },
  {
    "reference": "MQTYGNPDVTYGWWAGNSGVTNRSGKFIAAHAAHTGLIAFWAGAFTLFELARFDPSVPMGHQPLIALPHLATLGIGFDEAGTFVGGTTVTAIAIVHLVLSMVYGAGGLLHSLTFPGDMQDSEVLQARKFKLEWDNPDNQTFILGHHLIFLGVANIQFVEWARIHGIWDAAAGSIRQVEYNLNLSSIWNHQFDFLTINNLEDVMGGHAFLAFFMITGGAFHIATKQVGEYTKFKGSGLLSAEAILSWSLAGIGWMAIVAAFWCATNTTVYPVDFFGEVLDLKFGIAPYWVDTVDLPNGAHTSRAWLTNVHYFLGFFYIQGHLWHALRAMGFDFKRVSSAVSNIGTASVTLND",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. divinyl chlorophyll b is required for the proper folding and stability of the protein.2. A protein that contributes to photosynthetic electron transport chain by functioning in plasma membrane-derived thylakoid membrane, photosystem II, photosystem I with chlorophyll binding.3. The protein should have Helical domains to ensure proper insertion into the membrane.4. The protein must exhibit the following characteristics: The antenna complex functions as a light receptor, it captures and delivers excitation energy to photosystems II and I; The Prochlorales pcb genes are not related to higher plant LHCs.. The designed protein sequence is ",
    "response#1": "SCISMG",
    "response#2": "MPPPGFK",
    "response#3": "GCPCGSGCKC"
  },
  {
    "reference": "MAQAYWQCYPWLVLLCACAWSYPGPESLGREDVRDCSTNPPRLPVTAVNTTMRLAALRQQMEKSNLSAYIIPDTDAHMSEYIGKHDERRAWISGFTGSAGTAVVTKKKAAVWTDSRYWTQAERQMDCNWELHKEVSISSIVAWILAEVPDGENVGFDPFLFSVGSWENYDQELQDSNRHLLSITTNLVDVAWGSERPPVPSQPIYALPKEFTGSTWQEKVSAIRSYMQNHTMAPTGVLLSALDETAWLFNLRSSDIPYNPFFYSYTLLTDSSIRLFVNKSRFSLETLQYLNTNCTLPMCVQLEDYSQIRDGVKAYASGNVKILIGISYTTYGVYDVIPKEKLVTETYSPVMLIKAVKNSKEQALLKASHVRDAVAVIQYLVWLEKNVPKGTVDEFSGAEHIDQLRRNENFSSGPSFETISASGLNAALAHYSPTKELHRKLSLDEMYLVDSGGQYWDGTTDITRTVHWGTPTAFQKEAYTRVLMGNIDLSRLVFPAATSGRVVEAFARRALWEVGLNYGHGTGHGIGNFLCVHEWPVGFQYNNMAMAKGMFTSIEPGYYQDGEFGIRLEDVALVVEAKTKYPGTYLTFELVSFVPYDRNLIDVSLLSPEQLQYLNRYYQTIRENIGPELQRRQLLEEFAWLERHTEPLSASAPHTTSLASMWVASALAILSWSC",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The desired protein should be able to perform Release of any N-terminal amino acid, including proline, that   is linked to proline, even from a dipeptide or tripeptide.2. The designed protein should be involved in proteolysis by localizing to extracellular exosome, plasma membrane and exhibiting metalloaminopeptidase activity, metal ion binding.3. For general function, the protein need meet that May play a role in the metabolism of the vasodilator bradykinin.; Membrane-bound metalloprotease which catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro4. Include a signal peptide as an essential component of the protein.5. The designed protein should have a binding site that is specific to the target substrate, Zn(2+) ligand. The designed protein sequence is ",
    "response#1": "PDKLWPEGQVPYMLEEGMTNDQRTAIAQAFDEYKTKTCVRFVPKTDDDFDYIYVKRNVAFGCSSYVGRAGGNQTVSLEVDKCFSKGIIAHELMHALGFFHEHSRTDRDDFVDINEDNIRPGMMRNFEKYPRKIIDSLGMPYDYEFHYQEQTDPVEYVENMCENMQLYPLQDILTGDQLLFEYKPEVIGEALNQLVPQKANLVLLSGANEGKCDLKEKWFGTQYSIEDIENSWAELWNSNFELNPDLHLPAENKYIATDFTLKAFDCPETEYPVKIVNTPQGCLWYKKDNKFKIPKAYIRFHLISPLIQKSAANVVLFDIFVNILTHNLAEPAYEADVAQLEYKLVAGEHGLIIRVKGFNP",
    "response#2": "DVASASNFRAFELLHLHLDLRAEFGPPGPGAGSRGLSGTAVLDLRCLEPEGAAELRLDSHPCLEVTAAALRRERPGSEEPPAEPVSFYTQPFSHYGQALCVSFPQPCRAAERLQVLLTYRVGEGPGVCWLAPEQTAGKKKPFVYTQGQAVLNRAFFPCFDTPAVKYKYSALIEVPDGFTAVMSASTWEKRGPNKFFFQMCQPIPSYLIALAIGDLVSAEVGPRSRVWAEPCLIDAAKEEYNGVIEEFLATGEKLFGPYVWGRYDLLFMPPSFPFGGMENPCLTFVTPCLLAGDRSLADVIIHEISHSWFGNLVTNANWGEFWLNEGFTMYAQRRISTILFGAAYTCLEAATGRALLRQHMDITGEENPLNKLRVKIEPGVDPDDTYNETPYEKGFCFVSYLAHLVGDQDQFDSFLKAYVHEFKFRSILADDFLDFYLEYFPELKKKRVDIIPGFEFDRWLYDRCTGQSSDCPRLPCTPRFHYQEQTDPVEYVENMCENMQLYPLQDILTGDQLLFEYKPEVIGEALNQLVPQKANLVLLSGANEGKCDLKEKWFGTQYSIEDIENSWAELWNSNFELNPDLHLPAENKYIATDFTLKAFDCPETEYPVKIVNTPQGCLWYKKDNKFKIPKAYIRFHLISPLIQKSAANVVLFDIFVNILTHNLAEPAYEADVAQLEYKLVAGEHGLIIRVKGFNK",
    "response#3": "MIRKANDVTSQAHVAVLKAAKSATNERELEAAFIGTCIAQGCREMAYHPIVASGTSSATLHYVNNDEPLIDSSTNKKKLNLLLDAAGEYKAYCADVTRTFPLSGKFSPESREIYDIVLEMQTESLAMLKEGVLWEDVHITAHRVAIKGLLKLGILRGSEEELLEKRVSVAFFPHGLGHYLGMDTHDTGGHANYADKDKMFQYLRVRGKLPAGSVITVEPGVYFCRFIIEPYLKDSELSKYIDADVLEKYWEVGGVRIEDNIHITKSGYENLTTAPKIPEQLLDD"
  },
  {
    "reference": "MSQTPKHLAVVGATGAVGIEILKCLEQRDFPVSELTLLASARSAGKKLTFRGQEIEVKELTPDSFEGIDVALFSAGGGISKEFAPHAVKAGAVVVDNSSAFRMDEGVPLVVPEINPDDVSSNNGIIANPNCTTIVTLMALNPMHEAFGGVKSIIASSYQAVSGSGAQGIAELEGQVDCLAKGEPLPELSVYPCQIAFNVIPQVDVFQENGYTKEEMKMHFEGQKILHAPELKASVTCVRVPVFRSHAVAVTAVFNDKPSVAAAREAVSSAIGVRVVDDPFASPPQFPTPLDCTDGDDCLVGRIREDLVLDNALTFWVVGDQVRKGAALNAVQIAELL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be able to modulate lysine biosynthetic process via diaminopimelate, threonine biosynthetic process in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "LRQILDREDCVTMATIFDPVSARLAEQLGYEAGLMGGSLASYAVLGAPDLIVLTLTELAEQVHRCTRVSDVPLVVDGDHGYGNALSVMRTVHELDRAGAAAVTIEDTLLPRPYGSSGKPALVSFDEAVARVEAAVAARGDSDLLVLGRTSAATLNGIEDAVARFKAFEAAGVDAIFLPGPQQREQIDAISDAVKVPLLMAGAPEALCDPAYLATRRVKAWSAGHQTFSVALKALHDSMGAAE",
    "response#2": "LWPLHKACEIKRVVVTTFQSVSGTGTPAIEELRRQSEQVLEKGIEESCRHGLAKDAYPHQIAFNCLPHIDKFEEDGYTKEEHKMINETRKIFHEPKFRLTATTVRVPVFRGHSEAVNIEFKKAISPEEASAILKYAPGVTLIDDVSKNEYPMPIFCEWKDDVFVGRIRADKSVRYGLDMWVVADNILE",
    "response#3": "LWPLHKACEIKRVVVTTFQSVSGTGTPAIEELRRQSEQVLEKGIEESCRHGLAKDAYPHQIAFNCLPHIDKFEEDGYTKEEHKMINETRKIFHEPKFRLTATTVRVPVFRGHSEAVNIEFKKAISPEEASAILKYAPGVTLIDDVSKNEYPMPIFCEWKDDVFVGRIRADKSVRYGLDMWVVADNILE"
  },
  {
    "reference": "MAIQIILNFILAFIWIFLSGSYTLNNLLLGFILGLGFVYLFSRILPGRFYFIKIYKILKLAVVFFVELLKANIDVLKIVLQPKLKNEPGFFVYHTDLKTDWQIVLLSNLITLTPGTVVLGISDDRKKIYIHSIDFSTKEEEVEGIKSSLEKVVREVGED",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have antiporter activity within plasma membrane to regulate proton transmembrane transport. The designed protein sequence is ",
    "response#1": "VVSVPVGSALLGRVIDAMGRPRDTLGPIDAIAEHPIEAEAPSILNRTQIFRPLATGLKAIDAAIPVGLGQRELIIGDRQTGKTSIAIDTMLNQARSDVLCIYCAVGQRGDAVARVIRALKDGEMLQRCIVMSAGDEEAPGLSYIAPYAAMTMAEYFSAQGQDVLVIFDDMTHHARSYRELSLLLRRPPGREAFPGDIFYVHARLLERAGQFGADIGGGSITTLPVVETQAGNLSAYIPTNLISITDGQIYLSPQLVRKNQFPAVDLGLSVSRVGGSA",
    "response#2": "GLKAIDAAIPVGLGQRELIIGDRQTGKTSIAIDTMLNQARSDVLCIYCAVGQRGDAVARVIRALKDGEMLQRCIVMSAGDEEAPGLSYIAPYAAMTMAEYFSAQGQDVLVIFDDMTHHARSYRELSLLLRRPPGREAFPGDIFYVHARLLERAGQFGADIGGGSITTLPVVETQAGNLSAYIPTNLISITDGQIYLSPQLVRKNQFPAVDLGLSVTQNRTLRGVSGNLRVTLSQFEELEDFARFGTRLDDVTRARLARGSAVRTALRQAERDPMSALEQLAVLIAAMEGLFDQFNEQKINDAMRLVRDAAQQQLAVIEQRIIDNQTLQDDDHTLIVNCAQRGVSGNLRVTLSQFEELEDFARFGTRLDDVTRARLARGSAVRTALRQAERDPMSALEQLAVLIAAMEGLFDQFNEQKINDAMRLVRDAAQQQLAVIEQRIIDNQTLQDDDHTLIVNCAQKALSIAVALA",
    "response#3": "GLKAIDAAIPVGLGQRELIIGDRQTGKTSIAIDTMLNQARSDVLCIYCAVGQRGDAVARVIRALKDGEMLQRCIVMSAGDEEAPGLSYIAPYAAMTMAEYFSAQGQDVLVIFDDMTHHARSYRELSLLLRRPPGREAFPGDIFYVHARLLERAGQFGADIGGGSITTLPVVETQAGNLSAYIPTNLISITDGQIYLSPQLVRKNQFPAVDLGLSVT"
  },
  {
    "reference": "MVSIRLSRGGSKKRPFYHLTVTDSRKSRDGRFIERVGFFNPVARGQEERLRVESERLNYWLSQGAQCSERVAKLIKDAAAAA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that localizes to cytoplasm, ribonucleoprotein complex and can perform structural constituent of ribosome with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "MIF",
    "response#2": "ETVNLDRLWTLVPNETREKYLGKNTEVAPVINVLQSGYGKVLGKGRLPDTPVIIQTRYVSRRAEEKIKQAGGVVF",
    "response#3": "MPTVNLDRLWTLVPNETREKYLGKNTEVAPVINVLQSGYGKVLGKGRLPDTPVIIQTRYVSRRAEEKIKQAGGVVN"
  },
  {
    "reference": "MARYIFITGGVVSSLGKGLASAALGALLQARGYTVRLRKLDPYLNVDPGTMSPFEHGEVFVTDDGAETDLDLGHYERFTGVAARHTDSVSSGRIYQTVLDKERRGEYLGKTIQVIPHVTNQIKEFLEVGADEVDFMLCEIGGTVGDIEGLPFFEAIRQFGQEKPMGQCIFMHLTLLPYIAASGELKTKPTQHSVKELRSIGIAPDILVCRADQPIPDKERAKLALFCNVRPERVIAALDLKSIYEAPLAYHAEGLDQAVLDAFGIAPAPAPKLDRWHDVADRVKNPEGEVRIAVVGKYTQLEDAYKSISEALTHGGMANRVKVRVEWVDAEIFEADEPAPMLEGFHGILVPGGFGERGTEGKIRAAKYAREEKVPYFGICLGMQMAVVEASRNLAGIDKAGSEEFDNEAGQTRYEHVIYHMKEWVEGNRTIQRSIDDDKGGTMRLGAYDATLVDGSQVAEIYGTSAISERHRHRFEVDITYRQKLEDSGLTFSGLSPDGRLPEIVEIKDHPWFVGVQFHPELKSKPFEPHPLFADFVRAALEQSRLV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit ATP binding. The designed protein sequence is ",
    "response#1": "LEEFDQIINSTPGFVKGMWCGERECEDLVKEKTGATARCMPLEQEQLSDKCMCCGKPAKSMVYWGKAV",
    "response#2": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#3": "DFGQWYTDVIKKAELVDYSSVRGCMVIKPYGYAIWENIQKSLDTRFKETGHENVYMPMFIPESLLLKEKEHVEGFAPEVAWVTHGGDEKLTERLCVRPTSETLFCEHYSNSIQSYRDLPKLYNQWCSVVRWEKTTRPFLRTLEFLWQEGHTAHATAEEAQEETIRMLNVYADVLENVLAIPVIKGRKTEKEKFAGAHATYTVESLMHDGKALQSGTSHNFGDGFAKAFDIQYTDKNNQLQYVHQTSWGVTTRLIGAIIMS"
  },
  {
    "reference": "MGVRVAILAAGQGTRMKSDIPKVMHRVAGRTMVGWVIDAVASLDADVTVVTAPNADVLRSNLPGGVTSCIQQERLGTGHAVQVAIDHFGGVEGDAVLVLPGDTPLLTAETLQRLVETHLESEAAVTMLTTRLADPSGYGRVLRDREGHVTAIVEHGDATPAQLAVNEVNAAIYVFDGTPLAEALDGIDRDNVQGEYYLPDAVARFVADGLPVRAVEADRVEVSGVNSQHQLADVARTMRRRINEEWMAHGVWMADPDQVFIDTSVELAAGARLYPGVYLEGDTSIGQSAQIGPDTFITDSRVGPGAHVWYAVVRQAEIGEGAEVGPYVSLRPGTVLGPNSKAGTFVEMKNAVIGEKSKVPHLAYMGDVEVGVGANVGAGTITVNYDGYTKHRTVIKDGARIGSDTMLVAPVTIGENAFTGAGSTISRDVSDGALAVERAKQKEIPGYAAKREERHRRELEES",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein contains novel MobA-like NTP transferase domains that confer a unique function or activity.2. A protein that is capable of catalyzing reactions within the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "GATLIEPDSVFFSYDTDLGQDVTILPNVFFGPGVTIDDNVTVKSFSHLEGTTIGQEAQIGPFARLRPGADIGEGARIGNFVEIKKSVVEEGAKINHLTYIGDTFVGAGANIGAGTITCNYDGFDKHETHIGRGAFIGSNNCLVAPVRIADGAYTGAGSVISADVSEGALALTRVAG",
    "response#2": "LEDGVTLVAPETVFFAHDTHIGRDTVVEPNVVFGPGVTVESGATIRAFSHLEGCHVGARAIVGPYARLRPGAELGNGARVGNFVEIKSAEIGEGAKVNHLTYIGDANVGEAANIGAGTVTCNYDGVFKHRTEIGARAFIGSSTMLVAPVRVGEDAMTGSGSVITSDVPDGALGLGRARG",
    "response#3": "GATLTLPESVVFSHDTVLGQDVTVEPHVVFGPGVRVEDGATIRAFSHLEGCVVRAGAIVGPYARLRPGSDIGPAAHVGNFVELKNTVLGAGAKANHLSYLGDADIGAKSNIGAGTITCNYDGFVKSRTRIGEGVFIGSDSVLVAPVTVGDGAFTAAGSVITADVTPDAMAFGRARL"
  },
  {
    "reference": "MAHKKAGGSSRNGRDSIGRRLGVKKYGGEAVGAGNILVRQRGTKFWPGEGVGMGKDHTIFATVDGAVKFHKGLKKRTFISVLPVAEAAE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein localizes to the ribonucleoprotein complex. The designed protein sequence is ",
    "response#1": "MENTLAI",
    "response#2": "STKIRLARAGF",
    "response#3": "MGEA"
  },
  {
    "reference": "MERFRLASDYEPRGDQPRAIAELSEGIVRGDRDQVLLGVTGSGKTFTMANVIAAVNRPALVLAPNKTLAAQLYGEFRELFPDNAVEYFVSYYDYYQPEAYIPTTDTFIEKDSSINDEIDKLRHAATRSLLTRRDVIIVASVSCIYGIGSPAEYQAMHIFFHQGDEYGRDELLRKLVDIQYERNDLDFHRGTFRVRGDIVEIFPAHEGERAYRIEFFGDTVDAISEIDPLRGVVVQRLTKCAVYPASHYVATRETLDRAMEEIRTDLRERLQWFRERNMLVEAQRLEQRTMFDLEMMEEMGFCQGIENYSRYFDGRTPGEPPYTLLDYFPRDFILFVDESHITVSQVGGMYRGDRSRKETLVNYGFRLPAALDNRPLTFGEFTERLNQSVYVSATPADYELQQSGGVVVEQVIRPTGLLDPVIEVRPATEQVDDLLHEVRETTARGERVLVTTLTKRMAEDLTDYYRDLGVRVRYLHSDIDTIQRMQIIRDLRMGEFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSARSLIQTCGRAARNVNGRVIMYADTVTGSMQSCLDETARRRALQEAFNTEHGITPQTVKKGLRTILESIEERDYYTVAAVAETREEYISADEIPKRVKKLRKEMLDAAKKLEFERAADLRDQIKKLEEMELRLR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a binding site that is specific to the target ATP ligand.2. The designed protein must have at least one UVR, Helicase ATP-binding, Helicase C-terminal domain.3. The protein design must feature the Beta-hairpin motif.4. For general function, the protein need meet that This complex is subsequently bound by UvrC and the second UvrB is released; The UvrABC repair system catalyzes the recognition and processing of DNA lesions; A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities; If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed; Then UvrB probes one DNA strand for the presence of a lesion; DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands; Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer; If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.5. The designed protein should be optimized for excinuclease ABC activity, DNA binding, ATP binding, ATP hydrolysis activity activity in order to improve nucleotide-excision repair, SOS response. The designed protein sequence is ",
    "response#1": "EGIEKGLKMQTLLGVTGSGKTFTMANVIANVNKPTLVIAPNKTLAAQLCAEFREFFPENAVEYFVSYYDYYQPEAYLPATDTYIEKDSAINDEIDKLRHSATAALFERRDVIIVASVSCIYGLGDPQEYRELLLGEITKEEETEHLLKVGEKVDQRDMLRRLAEIQYTRNDVDFSRGTYRVRGEVVDIFPAESSTFAIRVEMFDDEIERLSIFDPLTGAIEKHIVRTTIYPKTHLSLRTGMTIDRNTLLRQLVDIQFDRNDIDFQRGRFRVRGDVVEIFPASRDDHAIRVEFFGDEIDRITEVDALTGEVIGTRDHVAIFPATHLNHLSELLKVGEKVDQRDMLRRLAEIQYTRNDVDFSRGTYRVRGEVVDIFPAESSTFAIRVEMFDDEIERLSIFDPLTGAIEKHIVRTTIYPKTHLNVLSEVTEGKEETQVDDLLSEIYKSVEKGERVLVTTLTKRMSEDLTDYLSEHNVKVRYLHSDIDTVERVEIIRDLRAGVFDVLVGINLLREGLDMPEVALVAILDADKEGFLRSARSIIQTVGRAARHLEGRAILYGDRITKSMAKAMDETARRREIQHAYNIEHGIEPHSLAKKILDSKLELVEKAEQGQVDDLLSEINLRAAIGERVLVTTLTKRMAEDLTEYLEEHGVRVRYLHSDIDTVERMEIIRDLRLGEFDALVGINLLREGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNGKAILYADKITPSMARAIGETERRRERQQAFNLAHGITPQALNKKVTDIEE",
    "response#2": "SEYQPSGDQPKAIAELAGRITAGETDVVLLGATGTGKSATTAWLVEAVQRPTLVLAHNKTLAAQLANEFRELLPNNAVEYFVSYYDYYQPEAYVPQTDTFIEKDSSINAEVERLRHSTTNSLLSRRDVVVVSTVSCIYGLGAAEEYLEAMVALQVGQNVGRDQLIRRFVAMQYERNDIDFSRGKFRVRGDTIEIIPVYEEHALRIELFGDEIEALYSLHPLTGEVLARTDAVSVFPATHYAASPATIQRAIGTIQTELHDRLVELEREGKLLEAQRLRMRTQFDIEMMEQIGFCSGIENYSRHIDGRAPGEAPHCLLDYFPDDFLVVIDESHVTVPQIGAMYEGDASRKRTLVEHGFRLPSAMDNRPLRWNEFKERVGQTVYLSATPGQYELGIADGVVEQIIRPTGLVDPQILVDSLQNQHRFQTLLGATGTGKTFTIAAVIEKIGRPTLVLAHNKTLAAQLCNELRQFFPNNAVEYFISYYDYYQPEAYIPVSDTYIEKSSSINDEIDMLRHSATRSLFERRDVIVVASISCIYGLGMPAEYLKAAISLTVGQEFDQRQLLRALVSVQLTLQTQQEISREDILKELVKMQYSRNDIEFIRGQFRVRGDVIEIFPINANYALRIELWGDEIDVIYKIDALKGDIIEELQKVIIFPAKHISLRKGMQKERDEIIKKLIEIQYERNDIDFSRGTFRVRGDLLDIIPSSTSSKGIRIEFFGDEIDRIREFDVLTGTIIGERNHVSIFPASHISLTVGQEFDQRQLLRALVSVQYNRNDLELTRGRFRLKGDILEIVPAYEDRVIKIDFFGDEIESIRYLDPLTGEVLQKLERISIYPARH",
    "response#3": "SEYQPSGDQPKAIAELAGRITAGETDVVLLGATGTGKSATTAWLVEAVQRPTLVLAHNKTLAAQLANEFRELLPNNAVEYFVSYYDYYQPEAYVPQTDTFIEKDSSINAEVERLRHSTTNSLLSRRDVVVVSTVSCIYGLGAAEEYLEAMVALQVGQNVGRDQLIRRFVAMQYERNDIDFSRGKFRVRGDTIEIIPVYEEHALRIELFGDEIEALYSLHPLTGEVLARTDAVSVFPATHYAASPATIQRAIGTIQTELHDRLVELEREGKLLEAQRLRMRTQFDIEMMEQIGFCSGIENYSRHIDGRAPGEAPHCLLDYFPDDFLVVIDESHVTVPQIGAMYEGDASRKRTLVEHGFRLPSAMDNRPLRWNEFKERVGQTVYLSATPGQYELGIADGVVEQIIRPTGLVDPQILVDSLQNQHRFQTLLGATGTGKTFTIAAVIEKIGRPTLVLAHNKTLAAQLCNELRQFFPNNAVEYFISYYDYYQPEAYIPVSDTYIEKSSSINDEIDMLRHSATRSLFERRDVIVVASISCIYGLGMPAEYLKAAISLTVGQEFDQRQLLRALVSVQSAFQPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSGKTFTIANVIADLNRPTMVMAPNKTLAAQLYGEMKQFFPENAVEYFVSYYDYYQPEAYVPSSDTFIEKDASVNEHIEQMRLSATKALLERRDVVVVASVSAIYGLGDPDLYLKMMLHLTQGMLIDQRSILRRLSELQYVRNDQAFARGTFRVRGEVIDIFPAEADDEALRVELFDDEVERLSLFDPLTGQLLRTVPRFTVYPKTHYVTPRERILQAMEAIRAELAMRRQSLLASNKLLEEQRLAQRTQFDLEMMNELGYCSGIENYSRYLSGRGPGEPPPTLFDYLPADGLLVIDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLRFEEFEALAPQTIYVSATPGNYELDKSGGEVIDQVVRPTGLLDPLIEV"
  },
  {
    "reference": "MENRWQVMIVWQVDRMRIRTWKSLVKHHMHISKKAKGWFYRHHYESHHPKISSEVHIPLGDGSLVITTYWGLHTGERDWHLGQGVSIEWRKRRYRTQVDPVLADQLIHLHYFDCFSDSAIRKTLLGRIVSPRCEYQAGHNKVGSLQYLALTALITPKKTKPPLPSVAKLTEDRWNKPRKTKGHEGSHTMHGH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.2. For general function, the protein need meet that Finally, Vif also contributes to the G2 cell cycle arrest observed in HIV infected cells.; Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism; In turn, they are directed to the 26S proteasome for degradation; May interact directly with APOBEC3G mRNA in order to inhibit its translation; Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G3. The protein should be optimized for stability and expression in host cell cytoplasm, host cell plasma membrane where it will participate in viral life cycle. The designed protein sequence is ",
    "response#1": "MEPVDPSLEPWNHPGSQPTTACSKCYCKVCCWHCQVCFLKKGLGISYGRKKRRQRRGTPQSSKDHQNPVPEQPLPTSRGNPTGPEESKKKVESKTETDRFD",
    "response#2": "MEPVDPSLEPWNHPGSQPKTACTNCYCKKCCYHCQVCFLKKGLGISYGRKKRRQRRRTPQGSQTHQASLSKQSAPQPRGDPTGPKESKKKVERETETDPVD",
    "response#3": "MEPVDPSLEPWNHPGSQPKTACNNCYCKRCSYHCQVCFLNKGLGISYGRKKRRQRRRPPQGGEDHQNLVSKQPLPQTQGDSTGSEESKKKVESKTKADPFD"
  },
  {
    "reference": "MNGSVGIVETKKVTFPEITLECGEKIAPVTVAYETYGELNERGDNAILILHALTGDAHVAGKHRPEDKVAGWWDPMVGPGRPFDTNKYFIVCSNVLGGCYGTTGPSSINPATGRPWGMSFPIITIRDMVNLQYKLVRHLGITKILAAVGGSMGGMQALEWAYMYPEMLKSVVAIATSARLSPFGIAFNAVGREAIMTDPEWRGGNYYGFEGPKRGLALARMIGIITYKSDISWQYRFGRTHTYETDQELFSHTSRFEIENYLYYQGDKLVKRFDANTYLYLLKAMDLHDISRGRGRYREILKELKTPLLAIGIDTDFLYPTYQQKEIVEALKEAKKEAYYWELSSPHGHDAFLIEFSKMAPILSNFLEYVAGRRATINF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a specific function within the cytoplasm.2. A protein that can modulate the overall flux of metabolites through the L-methionine biosynthesis via de novo pathway pathway, enhancing or suppressing its activity as needed.3. The protein contains novel AB hydrolase-1 domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "ESPVELTERLSDRLGVNFYVKREDRQRVFSFKLRGPYNMMSSLSHEEIDKGVITASAGNHAQGVPFPFPGRRLKCVAKIVMPTTTPNIKLDGVRALGADVVLWGHTFDEAKTHAVELCEKDGLRTIPPFEDPAVIKGQGTIGSEINRQIKRIDAVFVPVGGGGLIAGVAAFFKQIAPQTKIIVVEPYDAASMALSVHAEHRAKLSNVDTFADGATVAVIGEYTFARCQDVVDAMVLVANDGIGAAIKDVFDEGRNIVETSGAAGIAGMYCEMYRIKNDNMVGIVSG",
    "response#2": "CMSLYHFSDIYNCTNLISSIEDFASSQFRCIRKSPEFNSISFHHLKSLLNRSDLNVSEEQDVFETIVQWVSSNPRDRQHHFVQLFKTLRLHLVGWNFLCEAG",
    "response#3": "GGSAKVSDEVFVEFIIPEKADLKKFLEVLSPHWNLTLYRYRNQGDLKATILMVIASFLCEIVIRKNQIDDLGYPYEIDQYNDAFNV"
  },
  {
    "reference": "MSTVESSKPKIINGSCGYVLEDVPHLSDYLPGLPTYPNPLQDNPAYSVVKQYFVDADDSVPQKIVVHKDGPRGIHFRRAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSYMYGVKRILGIDGGYRGFYAKNTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDRGINQVYIIGGDGTQRGASVIFEEIRRRGLKVAVIGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESIENGIGVVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEKRLKESGHMVLVIAEGAGQDLMSKSMESMTLKDASGNKLLKDVGLWLSQSIKDHFNQKKMVMNLKYIDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYISGLVNGRQTYIPFYRITEKQNHVVITDRMWARLLSSTNQPSFLGPKDVFDNKEKPMSALLDDGNCNGVVDVPPVTKEITK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP, Mg(2+), substrate binding.2. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis..3. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-   bisphosphate + H(+) are properly positioned for optimal catalysis.4. The protein should be optimized for activity in glycolysis, D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 3/4, Carbohydrate degradation pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. The designed protein should have metal ion binding within cytoplasm, cytosol to regulate fructose 6-phosphate metabolic process, root epidermal cell differentiation, cellular response to hypoxia. The designed protein sequence is ",
    "response#1": "LGVVLSGGQAPGGHNVITGIFDGIKDVHPDSRLYGFKGGPKGIFTEKYIELTADFVNPYRNTGGFDMIGSGRDKIEKPEQLEACKNVCQKLALDALVIIGGDDSNTNAAVLAEYFVKENVRTCVIGVPKTIDGDLKNEVIETSFGYDTASKVYSELIGNICRDSKSAKKYWHFIKLMGRSASHITLECALQTHPNIVIIGEEVKAKNMTLQQIVESIAEVVKKRSEEGKNFGVCLVPEGLIEFIPEVRILISELNKILADHAARLNPRWKLKVGVLTGGGDCPGLNAVIRAVVRKGVFHYSDDFVGFLEGWRGLIENKTMPLDLSSVGGILHRGGTILRTSRTNPAKKSDGLDKCISTLNEHEIDALVAIGGDDTLSVAQKLHDKGVKVVGVPKTIDNDLSGTDYCFGFDTAVNVVTEAIDRVHTTAEAHNRVIVVEVMGRDSGWIAIQSGIAGGADVILIPEIPFDIEKVAETIRQRDARGRYFSIVVVAAGAKVDPQHGAPVIADMATDEFGHARLGGVANILAREIEHRTGFETRAVVLGHVQRGGTPSAFDRVLATRYGLGAIDMLVHGYSGYMASLKGNELITVSLAEAVKTTKLVPAEWLTMAKLFG",
    "response#2": "PGMNAAVRAVVRMGIYVGAKVYFIHEGYQGMVDGGDNIKEATWESVSSMLQVGGTVIGSARCKEFRSREGRLRAAHNLVKRSITNLCVIGGDGSLTGANLFREEWSSLLDELRQQGLIDEEAARSNSELHIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPEMPPEDGWEDSMCQKLSESRSRGSRLNIIIVAEGAMDRHGRPVTSDRVKDLVVRCLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVLALLEAGPDEMVALMGNRIEAVEVDLEAAREDSRLFATEYFRPVMDELSR",
    "response#3": "PGMNAAVRAVVRMGIYVGAKVYFIHEGYQGMVDGGDNIKEATWESVSSMLQVGGTVIGSARCKEFRSREGRLRAAHNLVKRSITNLCVIGGDGSLTGANLFREEWSSLLDELRQQGLIDEEAARSNSELHIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPEMPPEDGWEDSMCQKLSESRSRGSRLNIIIVAEGAMDRHGRPVTSDRVKDLVVRCLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVLALLEAGAEGVMAALRGDEVVRVPLAEAVAELKTVPAPRYEIAERFFG"
  },
  {
    "reference": "MGKPTGKKKNNNYTEMPPTESSTTGGGKTGKSFDRSATKSFDDDMTIFINRALELKEEGNKLFQKRDYEGAMFRYDKAVKLLPRDHGDVAYLRTSMASCYMQMGLGEYPNAINECNLALEASPRFSKALLKRARCYEALNKLDFAFRDSRVVLNMEPENVSANEIFERVKKVLVGKGIDVDEMEKNLVNVQPVGAARLRKIVKERLRKKKKKSMTMTNGGNDGERKSVEAVVEDAKVDNGEEVDSGRKGKAIEEKKLEDKVAVMDKEVIASEIKEDATVTRTVKLVHGDDIRWAQLPLDSSVVLVRDVIKDRFPALKGFLIKYRDSEGDLVTITTTDELRLAASTREKLGSFRLYIAEVSPNQEPTYDVIDNDESTDKFAKGSSSVADNGSVGDFVESEKASTSLEHWIFQFAQLFKNHVGFDSDSYLELHNLGMKLYTEAMEDIVTGEDAQELFDIAADKFQEMAALAMFNWGNVHMSKARRQIYFPEDGSRETILEKVEAGFEWAKNEYNKAAEKYEGAVKIKSDFYEALLALGQQQFEQAKLCWYHALSGEVDIESDASQDVLKLYNKAEESMEKGMQIWEEMEERRLNGISNFDKHKELLQKLGLDGIFSEASDEESAEQTANMSSQINLLWGSLLYERSIVEYKLGLPTWDECLEVAVEKFELAGASATDIAVMVKNHCSSDNALEGMGFKIDEIVQAWNEMYDAKRWQIGVPSFRLEPLFRRRSPKLHDILENVFSGPQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein have PB1 domains with specific sequence motifs that are crucial for its activity.2. For general function, the protein need meet that Carboxylate clamp type tetratricopeptide repeat protein that may act as a potential Hsp90/Hsp70 co-chaperone The designed protein sequence is ",
    "response#1": "GKSNILLVGPTGSGKTLLAETLARLLDVPFTIADATTLTEAGYVGEDVENIIQKLLQKCDYDVEKAQQGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTIASVPPQGGRKHPQQEFLQVDTSNILFICGGAFAGLDKVIRDRSEKGGIGFAAEVKSKDDKRNFGETLHDLEPEDLVRYGLIPEFVGRLPVIATLDELDKAALIQILTEPKNAYSNKEIFLRL",
    "response#2": "NKEIFLRELISNASDALDKIRYQALSDPSQLESEPELFIRIIPQKDQKVLEIRDSGIGMTKADLVNNLGTIAKSGTKSFMEALSAGADVSMIGQFGVGFYSLFLVADHVQVISKHNDDEQYVWESNAGGKFTVTLDETK",
    "response#3": "AKSNILVIGPTGSGKTLLAQTLARLLDVPFVMADATTLTEAGYVGEDVENIMQKLLQKCDYDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVASVPPQGGRKHPNQEFVQLDTTNILFICGGAFDGLEKIIRRRSEKGGIGFGAEVKSKEDARAIGEVLRDVEPEDLIKFGLIPEFVGRLPAIATLESLDEDALVTILVEPKNAR"
  },
  {
    "reference": "MKSATPDDLPRTGVEPDLSRSASECFESLWNGAIGMRSLPLTIAGARVLDAGVTCSGSLEAGLGLARLCLGDLANVRYVPATADDLVGLSVTIQTDHPVLSCLGGQYAGWPVSVADYFAMASGPMRCLRGKEAMLEQLHLSRQATDDDFAVGVLESDTLPGEDVIEAMADECGVDPSRLCLAVAPSTSIAGSAQVVSRSVETALHKLHALEFDVTRVVSAHGDAPLPPPAKKGDTIGGIGRTNDAMLYGARVTLWVDAEDDAIDSVASKVPSQSSDDHGRPFAEIFKQYEYDFYQVDPMLFSPAVVTIHSLQSGRTWRHGQISIDVMRKSFGL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein localizes to the cytoplasm.2. For general function, the protein need meet that Catalyzes the hydrolysis of methenyl-H(4)MPT(+) to 5-formyl- H(4)MPT.3. A protein that can modulate the overall flux of metabolites through the formaldehyde degradation, formate from formaldehyde (H(4)MPT route): step 3/5 pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MPPTRHPRP",
    "response#2": "MFLIQPSW",
    "response#3": "ME"
  },
  {
    "reference": "MNYALETNDKEIFDLIHEELDRQNTHLEMIASENFTFPAVMEAMGSVLTNKYAEGYPYKRYYGGCEFVDRVEEIAIERAKKLFGCGFANVQPHAGSQANVAVYNALLKPYDKILGMDLSHGGHLTHGAKVSVTGQTYQSFFYGVELDGYINYDKVEEIAKIVKPQMIVCGFSAYARELDFKRFREIADSVGALLLGDVAHVAGLIVAGEYPNPFPHCHIVTTTTHKTLRGPRGGMILTNDEEIAKKIDKAVFPGMQGGPLMHVIAAKAVGFGENLKPEWKEYAKQVKANAKVLAKVLMARGYTLVSGGTDNHLILVSLLDKEFSGKDADRALGEAGITVNKNTVPGETRSPFVTSGVRIGSAALTARGMREKEFEFIATKIADVLDDVNNAAKHAEIKKEIAEFAKGFPVYTKAIF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier; Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.. The designed protein sequence is ",
    "response#1": "LAKTDPDLWAAIQQEDRRQQDHIELIASENYTSPAVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKALFGAEAANVQPNSGSQANQAVFMAFLKPGDTIMGMSLAEGGHLTHGMALNMSGKWFNVVAYGLNEKEEIDYEAMERLAREHKPKLIIAGASAYALRIDFERFAKIAKEIGAIFMVDMAHYAGLIAAGLYPNPVPHADVVTSTTHKTLRGPRGGIILMKAEHEKAINSAIFPGLQGGPLMHVIAGKATAFKEAATKDFKRYQEQVIDNALVMCKVLVERGLRIISGRTESHVFLVDLRAKNLTGKEAEALLGRAHITVNKNAIPNDPQKPFVTSGIRIGTPAMTTRGFTELEAEQLAHLIAEVLDAPADLTVLGERVRETAFDPDIAALIDGELRRQESGLEMIASENYAPLAVMQAQGSVLTNKYAEGYPGRRYYGGCEFVDGVEQLAIDRVKALFGAEYANVQPHSGATANAATMHALLNPGDTILGLSLAHGGHLTHGMRINFSGKLYHATAYEVSKEDYLVDMDAVAEAARTHRPKMIIAGWSAYPRQLDFARFRAIADEVDAVLMVDMAHFAGLVAAGVHPSPVPHAHVVTSTTHKTLGGPRGGIILCNDPAIAKKINSAVFPGQQGGPLEHVIAAKATAFKMAAQPEFAQRQQRCLDGARILAGRLTQPDVAERGIAVLTGGTDVHLVLVDLRDAELDGQQAEDRLAAVDITVNRNAVPFDPRPPMITSGLRIGTPALAARGFSHNDFRAVADLIADVIDRLADRSPLGDAAVRRDVDPDAADALSSERQRQEDTLAMIASENHVSEAVLEAQGSALTNKYAEGYPGERYYAGCEYADEIESLAIERAEELWGAEHVNVQPHSGTQANMAVYLTALDPGDKILSLDLTHGGHLSHGHPANFTGQTYTVEQYEVDPETGYIDYEGLKTKADEFNPDIIVSGYSAYPREVEFERIQEAADLADAYHLADIAHITGLVAAGVHTSPVGVADFVTGSTHKTIRAGRGGIIMCNEEHADDIDNSVFPGAQGGPLMHNVAGKAVGFKEALSDEFEAYAEQTVKNAEELANTLTDAGLSVVSGGTDNHLVLVDLRPSHPETTGKEVEAALESAGIIMNANTVPGETRSAFNPSGIRVGTPALTTRGFTESTVREVGE",
    "response#2": "IEKTDPELFEAIKKEAERQEYKLNLIASENYASKAVMEAQGSILTNKYAEGYSGKRYYGGCDFVDIAEDLAIARAKKIFNAGYVNVQPHSGSGANMAVYFSVLKPGDTIMSMDLSHGGHLSHGSPVSFSGKLFNIVPYGVSKKTEMLDYSELMKKAKENKPQMIVCGASAYPREIDFKQFREIADEVGAYLLADIAHIAGLVVAGVHPSPVPYADFVTSTTHKTLRGPRGGIIISKTEELATRINKAVFPGLQGGPLMHIIAGKAVAFKEAMSEKFKQDQVQTVKNAKTLCKCLKEKGFDMVSGDTDNHLMLVNLNNMNITGKDAEAALSKAGIIANKNTVPFETRSPFITSGVRLGTPACTTRGMKETEMELIADYICEDMKEASEEVKALSLEKFKYAPCL",
    "response#3": "TAFDPDIAALIDGELRRQESGLEMIASENYAPLAVMQAQGSVLTNKYAEGYPGRRYYGGCEFVDGVEQLAIDRVKALFGAEYANVQPHSGATANAATMHALLNPGDTILGLSLAHGGHLTHGMRINFSGKLYHATAYEVSKEDYLVDMDAVAEAARTHRPKMIIAGWSAYPRQLDFARFRAIADEVDAVLMVDMAHFAGLVAAGVHPSPVPHAHVVTSTTHKTLGGPRGGIILCNDPAIAKKINSAVFPGQQGGPLEHVIAAKATAFKMAAQPEFAQRQQRCLDGARILAGRLTQPDVAERGIAVLTGGTDVHLVLVDLRDAELDGQQAEDRLAAVDITVNRNAVPFDPRPPMITSGLRIGTPALAARGFSHNDFRAVADLIAEALKNPADHAALAKVKARVDAFTAAFPVYG"
  },
  {
    "reference": "MIYEFKDGLEFAKELDRLDVLNSTRQRFYLNEGEIYMDGNSLGLSSKDAEAALQNVMNVWRKEAIKIWGVEDGRYFKFSRNLAEKLKSLINADAEEIIVMGSITSNLHQALATFYTPTEARYKILVDALNFPSDIYATKSMIELKGYEVEDVLVQVQSKDGRTLCEEDIIAEMKEDVALILLPSVLYRSAQLLDMELITKEAHKRGIIIGWDLAHSIGSVPHDFKKIDPDFAVWCSYKYLNGGPGANAGMYINRRHFGRNAGLKGWFGSKEESQFLMSHEFDQNNDANGFLLGTHNMFSMAPLDGSLNIFNEVGIETIRAKSLHITAYLMYLIDQKLSHHGYFIGNPREDSRRGGHVALEHDEAYRISLALRDHKVIPDYREPNVIRLAPVALYVSYEEVYRLVEILEKIVTHKEYEHYSLKRVTVL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein have enhanced H2O + L-kynurenine = anthranilate + H(+) + L-alanine.2. The presence of pyridoxal 5'-phosphate is necessary for the protein to function in the desired environment.3. A protein that can perform kynureninase activity, 3-hydroxykynureninase activity, pyridoxal phosphate binding in cytoplasm to support quinolinate biosynthetic process, anthranilate metabolic process, L-kynurenine catabolic process.4. A protein that can modulate the overall flux of metabolites through the quinolinate from L-kynurenine: step 2/3, Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed.5. The presence of Aminotransferase class V domains is critical for the interaction of the designed protein with its intended targets.6. The designed protein should have a high specificity for pyridoxal 5'-phosphate binding, with minimal off-target effects.7. The protein must exhibit the following characteristics: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively.. The designed protein sequence is ",
    "response#1": "EKPWWDYHERFAEPLSKIVGAKPSEITVMNTLTVNLHLLMASFYRPNATRYKIICEEKAFPSDQYMIQSQIQFHGFTTSQALVEVKKRENEHQLRIEDIFATIDAHANDTALLLMGGVNYYTGQVFDMQAITKYAQERGIVVGWDLAHAAGNIEMQLHDWNVDFAAWCSYKYKQPKSAREYMSRQMDNWENLAVEGWFEGATPWMFYHKELKALMANIVGASAAEVCPMNTLTVNLHLLMVSFYRPTAKRFKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVAPRAGEATLRTEDILDTIATNGAETALVLFGGINYFTGQWYDMPAITKAGHHAGAVVGFDLAHAAGNVPLQLHNWDIDFACWCSYKYQNGGPGGISGIFVHEKHFAIPEI",
    "response#2": "MSYHKSLKKKIAQLVGAKESEVCPMNTLTVNLHLLMVSFYKPVPGKFKIIMESGAFPSDQYALESQVRFHGYDPKEAIIEVAPRHGEYILRTEDILAAIAENGEELALVLFGGINYFTGQLFDLAKITEAGHEVGAVVGFDLAHAAGNVPLSLHDWKVDFACWCSYKYQNSGPGGISAIFIHENNFYHKSLKKKIAQLVGAKESEVCPMNTLTVNLHLLMVSFYKPVPGKFKIIMESGAFPSDQYALESQVRFHGYDPKEAIIEVAPRHGEYILRTEDILAAIAENGEELALVLFGGINYFTGQLFDLAKITEAGHEVGAVVGFDLAHAAGNVPLSLHDWKVDFACWCSYKYQNSGPGGISAIFIHENNLNIKNQPKSAREYMSRQMDNWENLAVEGWFEGATPWMFYHKELKALMANIVGASAAEVCPMNTLTVNLHLLMVSFYRPTAKRFKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVAPRAGEATLRTEDILDTIATNGAETALVLFGGINYFTGQWYDMPAITKAGHHAGAVVGFDLAHAAGNVPLQLHNWDIDFACWCSYKYQNGGPGGISGIFVHEKHFAEQTSKLVGSLPSEVVVMETLTANLHLLMASFYRPTADRYKIIIEGKAFPSDHYAAQSQLAHHNIPPSALITIEPPSSSSPYLSTEHILSIISQHAASTALVLLPGIQYYSGQLFDIELITRHCHSLGIMVGWDLAHAVGNVPLKLHDWNVDFAAWCNYKYMNGGPGVIGGLFV",
    "response#3": "MPLQPKSAREYMSRQMDNWENLAVEGWFEGATPWMFYHKELKALMANIVGASAAEVCPMNTLTVNLHLLMVSFYRPTAKRFKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVAPRAGEATLRTEDILDTIATNGAETALVLFGGINYFTGQWYDMPAITKAGHHAGAVVGFDLAHAAGNVPLQLHNWDIDFACWCSYKYQNGGPGGISGIFVHEKHFAGKHPEGQTQPKSAREYMSRQMDNWENLAVEGWFEGATPWMFYHKELKALMANIVGASAAEVCPMNTLTVNLHLLMVSFYRPTAKRFKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVAPRAGEATLRTEDILDTIATNGAETALVLFGGINYFTGQWYDMPAITKAGHHAGAVVGFDLAHAAGNVPLQLHNWDIDFACWCSYKYQNGGPGGISGIFVHEKHFAGKKPEGKAQPKSAREYMSRQMDNWENLAVEGWFEGATPWMFYHKELKALMANIVGASAAEVCPMNTLTVNLHLLMVSFYRPTAKRFKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVAPRAGEATLRTEDILDTIATNGAETALVLFGGINYFTGQWYDMPAITKAGHHAGAVVGFDLAHAAGNVPLQLHNWDIDFACWCSYKYQNGGPGGISGIFVHEKHFAGKKPQGKAPDGQAPEGYTPEGYTPEGYTPEGYTPEGYTPEG"
  },
  {
    "reference": "MGKINAENTSKHVTFSKNAFIPVCNWCRNVCGYCTFRNENFKLLKMDEMKEILTKADTFGCREALFTFGENVDENEKVKEELKKMGYSGILEYLYEISAWCLENTNLIPHTNCGILSYDELKYLREVNASMGLMLENSSARLCGTIAHEKSPGKDPKLRIEMIENAGKLKIPFTTGILIGIGETFEERVNSIFEIKRIHEKYGHIQEVIVQNFRSKPQIPMENYKEPSPVEMFKMIILSKLILEDISIQVPPNLNRETGQLFLMAGIDDWGGVSPLTKDFVNPEAPWPDIEELNIFSKELGFTLKERLPVYEKYITEEWVDKKILEKIKK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity.2. The protein should have a low Km value for 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-   dihydrouracil + S-adenosyl-L-methionine = 5'-deoxyadenosine + 7,8-   didemethyl-8-hydroxy-5-deazariboflavin + H(+) + L-methionine +   NH4(+).3. The [4Fe-4S] cluster binding site should be stable and able to withstand changes in environmental conditions.4. The protein must exhibit transferase activity, transferring alkyl or aryl (other than methyl) groups, ammonia-lyase activity.5. For general function, the protein need meet that Catalyzes the radical-mediated synthesis of 7,8-didemethyl-8- hydroxy-5-deazariboflavin from 5-amino-5-(4-hydroxybenzyl)-6-(D- ribitylimino)-5,6-dihydrouracil.6. [4Fe-4S] cluster is required for the proper folding and stability of the protein.7. The protein design should take into account the physiological conditions of the coenzyme F0 biosynthesis, Cofactor biosynthesis pathway. The designed protein sequence is ",
    "response#1": "EDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAREIGLEKETGRRPITNVVMMGMGEPLLNMKNLIPALEIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIGQIDVALAISLHAPNDKLRSEIMPINDRWNIEAFLEVVRRYIASSNANRGKVTVEYVLLDHVNDGTEHAHELAELLKGTPCKINLIPFNPYPGSPYKKPSNSRIDRFQKTLMQYEHTVTIRKTRGDDIDAACGQLVGRVQDRTYYTRKRRDIGIGVDL",
    "response#2": "SSQVGCTLTCRFCHTGTQRLVRNLDAREIVSQFLVARDSYGEWPSPGGGRLISNIVMMGMGEPLFNYENVAKAIRIVMDEEGLSISRRRITLSTAGVAPMIERCGAELGVGLAISLHAVTDELRDELMPINRKYPLSQLLDACRRYPGASNARRITFEYVMLKGINDSPHSIVGEVNSKIPEDLMERAYQGKCTKEDALQLLEVPPFELFRFADELRDLAVGDTVTYVVNRNINFTSRCVGTCGFCAFRTNNGKVLSIEEIMEKVRDAEKANATEVCIQGGLLPEVGLDFYQGIAEAIKAEFPEMHIHSFSPMEVYHASRISEIPVKEALRRLKRSGLDTMPGTAAEILSDRVRKIICPSKLKTAEWVEVVRQAHAAGIPTTATMMYGHVETREERIDHMLIIRDIQKETGGITEFVPLPFMPYNNPVGEKMIREGRYATPGLEDLKIYAVSRILFHGHVDNIQASWVKLGKKLAQFSLHCGTNDLGGTLMEESISRSAGACHGEMITVDELEWMIHGAGRIPKERTTLYRRVTSSNPYF",
    "response#3": "ISTQAGCALGCTFCATGQQGFRRNLTFGEIVKQVLYMRQLALSEDMKEVAKGSRTKGEIQGVTNIVFMGMGEPLANYDNIISAIRILNDPLGLNIGARHITVSTVGLVPQILKLSEEKIQINLAVSLHASDNETRSSTMPINKRYPIEELINACKKYISKTGRRIFFEYVLLSEHNDSEEHAYISFENINEDEIKERAYQGTTTKEDALTLLEIEPFELFELADQIRAKAVGDNVTYIVNRNINFTDICIGTCGFCAFKDKKGYLLSIDQIKDKIKEAHVSGATEVCIQGGLLPNVKIDLYIDILKAVKSDYPHIHTHCFSPMEVNHAAKASGLSVEETLKTLKANGLNTMPGTAAEILVDKVRNIICPDKLTRQEWIDTVTLAHKLGIQTTATMMYGHVDTWEDRIEHILTIRRIQKDTGGFSEFVPLSFMPYNNPIGEKMMEEGRFMNTGIDDLKIYAIARILLNTHINNIQTSWVKLGKKLAQMALYCGANDMGGTLMEESISSSAGASSGEAISAEELEWIIRATDRKPVQRDTLYRSEWNQKNRYNERLVKASK"
  },
  {
    "reference": "MQDWREVNHRTLPQQTTTNRQVERRLDEAGLEPRWRGVPPAEFPALRSDSRLVGAGDLFCAIPGTRVDGHAFVEAAQRAGAGAAVVERAADVDLPQLSVRDARAAVSHLAALFAGDPGEALRLIGITGTNGKSTTAWLTRWILHDVGPAAALGTLGTVSIDGEVGAPGLTTPDPIDLALELAALRAEGAEAAVLEVSSHALDQRRADGFSFDAVGFTSFSREHLEYHPDLEAYREAKLRLLDLLAPGGVCAVNDDEPAWKAVAPENATTLRYGFEPTADLWPDRLVLHAGGARFRLRAPGEAADVSLPLPADFNVRNALAAAAIAWGLGMPLERIAARLSAAPAVPGRMEVLSREPVLVIRDFAHNPDSCERALSSLRAIVPGRVIALLGCGGDRDPGKRPQMGSILARLADVAIVTSDNPRSEDPSEICRQMVVGLPPDSYEIVVDRRAAIARGLAEAGPADAVALLGKGHETYQVIGGKRLPFDERRIVEDLLPALPGTTGLRGGTA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..2. The desired protein should be able to perform ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate3. The protein design should consider the endogenous proteins and enzymes present in the Cell wall biogenesis pathway to avoid interference with native metabolic processes.4. The protein contains novel Mur ligase N-terminal catalytic, Mur ligase C-terminal domains that confer a unique function or activity.5. The designed protein should have a binding site that is specific to the target meso-2,6-diaminoheptanedioate, ATP ligand.6. The designed protein must be able to regulate cell wall organization, cell division. The designed protein sequence is ",
    "response#1": "MRLRDLAVTDEHGAADVVLTSDSRKVTAGSLFVAYPGDAADGRAYIPQAIANGAGAVLWEAEGFSWPADLQAVNLPVKDLRKQAGEIADEFACARFPRQHPGVVAIGVTGTNGKTTTTNFIERILSASGQRAGLIGTLGTWHGARQADTDRTTPDALTLQRLLAEFAAQGVGASAMEVSSHALAQARVAGVRFDVAVFTNLTQDHLDYHGDMESYFAAKKRLFTPDQTGRAVINIDDPHGRRLAEQAPVVTISTDQPDADVRATDIRFAADGTRFTLHGPDGDAAVSVRLPGRHNVMNALAAIAVCRALGVAPDDAARGLAAVSRVPGRVQRIDRGQNFLAVVDYAHKPAAVEAVLATLRESSRGRIAVVLGAGGDRDTAKRPLMGAAGARGADLLVVTDDNPRSEDPATIRQAVQDGALSVPEDARGEVRNVAGRAAAIEQAVVWAREGDVVLIAGKGHETYQDVHGVRHPFDDREVARAALIERWTSAAAAP",
    "response#2": "MKLSELLTKVVPVAVRGNTDCGIVHITADSRKVGVGSLFVALTGDHVDGHDFVLQAEEQGATAVVVERMPDDLPAVHIPNPRRDLALLASAVYNHPARELFVVGVTGTKGKTTTTYLLEEILRAAGSAPGVIGTIEYRRPGWKIPAPRTTPEAPDLQALLKDMLDAGVSHCVMEVSSHALEQKRVWGVSYDVAVFTNLTGEHLDYHPSMEGYFEAKRKLFTLNHKRSAAVVNMDDPWGQKLVAELPMKTVTFGLEPAAIVRAVKHAMSEDGIDAQVSYPGGTMRIQSALVGRHNLYNLLAAAAAALVHGVALHTVVRGIESCRVVPGRMELIHEKQPYNVLVDYAHTDDALKNATGMLREITPGKLIVVFGCGGDRDRSKRSLMLKATLEHADQIFATSDNPRSESVEQIFEDMRLGNETSLDRVEFIEDRKHAISRALDAAQAGDCVLIAGKGHETYQIIGDRRIAFDDRQVAREVLAGRL",
    "response#3": "MKKHVAGLQQDSRKVKQGDCFIAVTGLETDGRRYIASALERGASVVLQEADQQQHGDVAFLSSRPIISVSNLLQKMSRIAANFYGRPSESMTMIGVTGTNGKSSVVSFLRQIWCLNGRSAASLGTLGLESAPVSIPLTLTTPDPPVIHDQLARLATHGVNHLAIEASSHGLDQHRIDGVNVNIAGFTNLSRDHLDYHGSLAQYLSAKARLFGDVMAPGGVAVLNRDTPCYPRLMEICRERDHRVIQYGQGVRKDKGDIGNIDLIDVKATPDGLRLSVGYMGQTAVVEVSLLGKFQAENLLCAVGLAIATGIPVEAALTALAKVKGVAGRLETFESAGLTVIVDYAHTPDALDKALQGVRQHCSGKLVCVFGCGGDRDRGKRPEMGAIAASRADKLLVSSDNPRNENPEQIIDDILAGIDANQCELLVEVDRRMAIERAIHEAAAGDCVLIAGKGHEDYQIIGKEKHHFDDREEARKALQKKEERSV"
  },
  {
    "reference": "MLDQKTIDTVKATAPILAATGPKLTAHFYDRMFAHNPELKDIFNMSNQRNGHQREALFNAICGYATNIDNLGALLPVVEKIAQKHTSFMITAEQYNIVGTHLLASIDELLSPGEEVITAWGKAYGVLADIFIQREEQIYSEIETSDGGWRGLRDFKVVSKTVESSVITSFVFEPIDGKPVIGFTPGQYLGIYLNSDAFDNQEIRQYSLSAAPNGKTYRISVKREDQGVVSNYLHQSLQVGDTVQLAPPAGDFFLQAESENPIVLISAGVGLTPMLSMLETLQGREQSVTWIHAAENGEQHAFKDNVEQVCKQQNFQHLTWYRSPLSSDVQAEDYQYEGLIDLSKLNSTQLPSNAEVYFCGPVPFMQFAAKQLVEQGINQDQIHYECFGPHKVL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have oxygen carrier activity, heme binding to facilitate response to nitrosative stress.2. The protein design should incorporate a binding pocket that specifically recognizes heme b to enhance enzymatic efficiency.3. For general function, the protein need meet that Therefore, plays a central role in the inducible response to nitrosative stress.; Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds The designed protein sequence is ",
    "response#1": "MLSEQTKDIIKSTVPVLEVHGTAITTTFYRNMFEAHPELLNIFNHANQAKGRQQTALANTVYAAAANIDRLEAILPAVVQIAHKHVSLGIKPEHYPIVGEYLLKAIKEVLGDAATDDIINAWAEAYGVIADVFISVER",
    "response#2": "MLSQKTIEIVKSTVPVLEEHGTTITTCFYQQLFENHPELLNIFNHANQKKGRQQQALANAVYAAAAHIDQLETILPVVKQIAHKHRSLGIKPEHYPIVGEHLLLAIKEVLGDQATDEIIEAWGEAYGVIADVFIQVER",
    "response#3": "MLSQQTIAIIKSTVQVLEVHGTEITTTFYQSLFNAHPELLNLFNHANQKQGKQQAALANAVYAAALHIDRLEAILPVVKQIAHKHRSLGILPEHYPIVGQHLLGAIKTVLGDAATDDMIGAWGEAYGVIADAFLG"
  },
  {
    "reference": "MGGKWSKRSGGGWFAVRERMRRTPPAEPAADGVGAVSRDLERHGAITSSNTATNNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVDPDQVEKANEGENNCLLHPMSQHGMDDPEKEVLMWKFDSRLAFHHMAREKHPEFYKDC",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should include a Diacidic; necessary for CD4 internalization, Dileucine internalization motif; necessary for CD4 internalization motif to ensure proper function.2. The ideal protein would be one that plays a critical role in suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host autophagy, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II through GTP binding, SH3 domain binding within host cell plasma membrane, membrane, virion component, host cell Golgi membrane, extracellular region.3. For general function, the protein need meet that Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1 The designed protein sequence is ",
    "response#1": "MGGKWSKRSGAGWPTIRERMRRTEPAAEPAAVGVGPASQDLDKYGALTSSNTSSTNADCAWLEAQEDEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIFSQKRQDILDLWIYNTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPEKVEEANEGENNSLLHPMSLHGMEDPEKEVLEWRFDSRLAFHHMARELHPEYYKNC",
    "response#2": "MGGKWSKRSEGWSAVRERMKRAEPAAEGVGAASRDLEKYGAITSSNTAATNADCAWLEAQEDEEVGFPVRPQVPLRPMTYKGAVDLSHFLKEKGGLEGLIHSQRRQDILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPEKVEEATEGENNSLLHPICQHGMDDADREVLVWRFDSRLAFHHMARELHPEYYKDC",
    "response#3": "MGGKWSKSSLVGWPQVRERMRRAEPAADGVGAVSRDLEKHGAVTSSNTAATNAACAWLEAQEEEEVGFPVRPQVPLRPMTYKAAIDLSHFLKEKGGLEGLIHSQKRQEILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPEKVEEANEGENNSLLHPMSLHGMDDPEKEVLVWKFDSRLAFHHMARELHPEYYKNC"
  },
  {
    "reference": "MSSFIRWYNSRLAARPLLTQSVTTAFLFATGDVTAQQLVEKRGAQKHDLVRTGRMALYGGFVFGPVATTWFAFLARRVNVRNNKKAEVLARVACDQLGFAPVMIGVFLSSMATMEGKSVKERIDKTWWPALKANWMVWPAVQVINFSLIPLQYRLFFANIIAIGWNSYLSWVNSQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "VLFWLCILSFFSVLNEMVLNVSLPDIANHFNTTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNHFFILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGGIIAHYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYNWTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGGLIFSIVAGFISMVPYMMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVFILGSLSISISFLTIAFFVEFSMWLTTFMFIFVMGGLSFTKTVISKIVSSSLSEEEVASGMSLLNFTSFLSEGTGIAIVGGLLSLQLINRKLVLEFINYSSGVYSNILVAMAILIILCCLLTIIVFKRSK",
    "response#2": "VLFWLCILSFFSVLNEMVLNVSLPDIANHFNTTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNHFFILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGGIIAHYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYNWTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGGLIFSIVAGFISMVPYMMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVFILGSLSISISFLTIAFFVEFSMWLTTFMFIFVMGGLSFTKTVISKIVSSSLSEEEVASGMSLLNFTSFLSEGTGIAIVGGLLSLQLINRKLVLEFINYSSGVYSNILVAMAILIILCCLLTIIVFKRSE",
    "response#3": "VLFWLCILSFFSVLNEMVLNVSLPDIANHFNTTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNHFFILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGGIIAHYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYNWTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGGLIFSIVAGFISMVPYMMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVFILGSLSISISFLTIAFFVEFSMWLTTFMFIFVMGGLSFTKTVISKIVSSSLSEEEVASGMSLLNFTSFLSEGTGIAIVGGLLSLQLINRKLVLEFINYSSGVYSNILVAMAILIILCCLLTIIVFKRSV"
  },
  {
    "reference": "MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHAKSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYFDEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMKNGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIEDAIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLVDEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENLQPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDFMEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGRKVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQIKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a binding site that is specific to the target ATP, L-glutamine ligand.2. The protein design should include a 'HIGH' region motif to ensure proper function.3. The designed protein should have ATP binding, glutamine-tRNA ligase activity to facilitate glutamyl-tRNA aminoacylation. The designed protein sequence is ",
    "response#1": "DAYISVHEALKSAAAWWRVKLSIIDIDSEKLEKKDKKEWQRLESVQGVVVPGGFGERGIEGKIMASEYCRKNKVPYFGLCLGMQIMSIAFGRNLLKTKEVNSTEFDVKVKHPVIHIMPEQRKNLADKHMGASMRLGAYDCDLAINSLAYKAYTKKDSSAVPVTNKRGQVTISERHRHRYEFNNDYRKKFASAGFIISGVNPQKNLVEIAEIKDHPFMLGTQFHPEFQSRPLRPHPLFREFILKDAYKSLDEALTHGGIQNKVKINLIRIESDKLKSNNIRKKLKNVSGILIPGGFGKRGTEGKIACIKYAREKKIPFLGICFGMQMAVIEFARNVLKIKNAGSTELDKRIYPVIGLINEWSKDGLIIKGTDRNLGGTMRLGIYEALLKNNSAIKKIYKSSKIKERHRHRYEVNNNLREKFEKNGLIFSGMSPDNKLPEIIELNSHPWFIGVQFHPEFKSRPLDPHPLFSSFIS",
    "response#2": "ITVALVGKYTAHGDAYISVHEALKSAAAWWRVKLSIIDIDSEKLEKKDKKEWQRLESVQGVVVPGGFGERGIEGKIMASEYCRKNKVPYFGLCLGMQIMSIAFGRNLLKTKEVNSTEFDVKVKHPVIHIMPEQRKNLADKHMGASMRLGAYDCDLAINSLAYKAYTKKDSSAVPVTNKRGQVTISERHRHRYEFNNDYRKKFASAGFIISGVNPQKNLVEIAEIKDHPFMLGTQFHPEFQSRPLRPHPLFREFVGHA",
    "response#3": "VNIAIIGKYVNLKDAYKSLDEALTHGGIQNKVKINLIRIESDKLKSNNIRKKLKNVSGILIPGGFGKRGTEGKIACIKYAREKKIPFLGICFGMQMAVIEFARNVLKIKNAGSTELDKRIYPVIGLINEWSKDGLIIKGTDRNLGGTMRLGIYEALLKNNSAIKKIYKSSKIKERHRHRYEVNNNLREKFEKNGLIFSGMSPDNKLPEIIELNSHPWFIGVQFHPEFKSRPLDPHPLFSSFL"
  },
  {
    "reference": "MPVKYVFVTGGVVSGLGKGITAASLGRLMKERGYKVTMQKFDPYINIDPGTMNPIQHGEVFVTDDGTETDLDLGHYERFIDESLDKRSNVTTGKVYWSVLSKERRGDFGGGTVQVIPHITNEIKSRFYRNDGSNETHIAIIEVGGTVGDIESQPFIESIRQFQHDIGHENAILIHVTLVPYLHASEELKTKPTQASVRELQALGIQPDIVVCRTEKPLDENIKEKISLFCNVPKKNVIQNLNLPTVYAAPLAMEEEHLADDVCDLLHLENQKPDLTEWASMVNKLLNPKKTAHLALVGKYVALHDAYISVVEALKHGAIAHEADIDIKWLSSEDITPDNVEEMLGDVDGIIVPGGFGQRGIDGMLTSIRYARENGVPYLGLCLGMQLSIVEFARDVLGYSDANSTEQDPETTHPVIHIMPDKDGIEDIGGTLRLGSYPCHLEKGSKAHELYGEDVIYERHRHRYEVNNYYRDDLTKAGMKLSGLSPDGHIVEMVELPDHPWFVATQAHPEFKSRPNRPHPLFRGFIEAILKHAEA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design must be able to perform ATP binding, metal ion binding, glutaminase activity in order to contribute to glutamine metabolic process, 'de novo' CTP biosynthetic process.2. The protein should be optimized for activity in Pyrimidine metabolism, CTP biosynthesis via de novo pathway, CTP from UDP: step 2/2 pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.4. A key design requirement for the protein is the optimization of the Nucleophile, active site, Nucleophile; for glutamine hydrolysis to achieve high catalytic efficiency for H2O + L-glutamine = L-glutamate + NH4(+).5. The L-glutamine, Mg(2+), ATP, CTP, UTP binding site should be located in a region of the protein that is accessible to the ligand.6. The presence of Glutamine amidotransferase, CTP synthase N-terminal domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "KFIFVTGGVVSGLGKGITASSLGALLKASGLKVFMQKFDPYLNVDPGTMSPYQHGEVFVTSDGGETDLDLGHYERFTDEELSKISSTSAGKIYLEVIEGERRGDSQGKTIQVVPHITDAIKHKVYLAAQRSKADVIISEIGGTVGDIESQPFIEAIRQIRMEQGKENVMFIHVVLLLWLAASKEYKTKPIQHSVRELLSLGIQPDVIVSRSDKSSPQEIKEKVSLFCNVPTENIIDAVDQDSIYRVPLAMAEQNLHKLVIDQLKLKDSYISIIEALKHASAYLGVKPVLKWIESTDLEIDSDITKYLHDIEGAIILPGFGKRGSEGKIKAIRFLRENNIPTLGICFGFQLMVVEIARNVAGIVKANSSELDPSTPDPVIDLLPSQRNLDVLGGTMRLGSRKIRLLKYTKAFDAYKAEYIYERHRHRYSVNNKYLDVFESVGFIVSGISDEEDVIEFMELKNHRLFIGTQAHPEFKSRPLSPSPIYKYFIEEYPKKIKKRKVKIGMIGKYTKLKDSYISIIEALKHASAYLGVKPVLKWIESTDLEIDSDITKYLHDIEGAIILPGFGKRGSEGKIKAIRFLRENNIPTLGICFGFQLMVVEIARNVAGIVKANSSELDPSTPDPVIDLLPSQRNLDVLGGTMRLGSRKIRLLKYTKAFDAYKAEYIYERHRHRYSVNNKYLDVFESVGFIVSGISDEEDVIEFMELKNHRLFIGTQAHPEFKSRPLSPSPIYKYFLDRL",
    "response#2": "KYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTHLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTSAKMGKRNNFTTGQIYESVIKKERRGEYLGKTVQVIPHITDEIKLYLKRGAEGSDVAIVEVGGTVGDIESLPFLEAIRQMGLEEGRQSSCFIHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDILLCRADRSVPADERRKIALFCNVMPEAVIECLDADSIYRIPGMLHDQMLDEIVKYIFITGGVLSSLGKGLAAASIAAVLECRGLRVTNQKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTSTRMGKSNNLTTGQVYFSVITKERRGDYLGKTVQVIPHITNEIKDYIRQSSEGFDVALIEIGGTVGDIESLPFLEAIRQFRNEVGKQNAIFIHLTWVPFIKTAGEVKTKPTQHSVKALREIGIQPDILLCRTEEFLSEDIKAKIALFCNVEEAAVFTAKDVSCIYEVPLIYHREGLDQKIVDLLNLQDAYKSIYESLTHAGGANDCGIELVVVDAEDIEREGAEKYLSKVDGILVPGGFGDRGTEGKIAATRYAREHEVPYLGLCLGMQIATIEFARNVCGLAKSNSTEFDPQTPHPVICLLEEQKEISDKGGSMRLGTWPALLKPGSLAHRVYGRTEIAERHRHRFEFNVAYREQMEAAGLVVSGTSLDGSLVEMVEIPNHPWFLAVQFHPEFQSKPNMPHALFKGFINSKLSHRDADKSLCEALTHGGIANDTRVNIEWIEAESFEKEPHTVAERLKDKKAILVPGGYGERGTAGMISAIQYAREHQIPYMGICFGMQLAVIEVARHLAGIEKANTTEFGVTAEPVVGLLTEWLNGDVKERRSAEDDLGGTMRLGLYPAILAPGSLIQKIYGTSSIQERHRHRYELNIEYREKFEKAGLKFSGLSPDGLLPETVELEGHPWFVGVQFHPELRSRPFDPHPLFTSFI",
    "response#3": "MAKYIFVTGGVVSSLGKGLASASLGTLLERRGLRVALQKLDPYLNVDPGTMNPLQHGEVYVLADGAETDLDLGHYERFTNSLLTRANNVTSGQVYETVLSKERRGDYLGKTVQVIPHITNEIKARIRHFEETSPADILITEVGGTTGDIEGLPFLEAIRQFILQVGSENAILMHVTLVPFIKAAGELKTKPTQQSVAKLREIGLQPQVLLCRTEHPLDREVREKLSLYCNVPLDGVIEARDVEHSIYEFPLMLQQEGLDEKVCRLFELLAIEAPPPDLTQWQTLQDAYKSILESLAHAGTYNNRKVKTHFINSEHITRENVGDQLKDMQGIVICPGFGTRGIDGKIIAAEYTRTHDIPTFGICLGMQMMVIEFARNVLGYKDANSREMDEQTPHNVIDIMEEQKSITQMGGTMRLGAYECELVEGSRTWEAYDKETLIRERHRHRYEFNNQYLEEYEAKGMKCVGINPAANLVEIVEIPNLRWYIGTQFHPEYSSTVLNPHPLFMSFIEAALSAR"
  },
  {
    "reference": "MTEFSASLAPQVEAFLRYLSIERQLSPLTVTSYRRQLSALMEIGEQMGLAHWQTLDAAQVRSLVSRSKRAGLHASSLALRLSALRSFLNWLVSQGVLPANPAKGVSTPRSGRHLPKNIDVDEVNKLLSIDLNDPLAVRDRAMLEVMYGAGLRLSELVGMNCKHVDLASGDVWVMGKGSKERKVPLGKTAVTWLQHWLALRELFEPQDDAIFLANTGKRISARNVQKRFAEWGVKQGVSSHIHPHKLRHSFATHMLESSGDLRAVQELLGHANLTTTQIYTHLDFQHLATVYDAAHPRAKRGKS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein have Tyr recombinase domains with specific sequence motifs that are crucial for its activity.2. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The designed protein must possess tyrosine-based site-specific recombinase activity to support cell division under certain conditions. The designed protein sequence is ",
    "response#1": "FMSKEELKKFLDAIENADFKNNTIRNKLIIKIIIFTGIRVSEAINIKMGDISEENDLYIIRIRAKGNKYRVVMIKKELIYDLLKNVSINYISKDALLFVNKKGTPLTQSYVSRIVEQLLFRAGIRKQKNGAHMLRHTFATLLYKKQKDLVLVQEALGHASLNTSRIYTHFV",
    "response#2": "LTKGVKNIDELDALAKKARNAGLSGVNTYFNPLKKVFEYLNFYKLYSLKQIDEELIVEVLASITGALSDASKKNYRIAVINFFDFLK",
    "response#3": "DCEENFEKSFLFWLAKYVKFKLNSLSNKELKNPQALAEVNFALTKGVKNIDELDALAKKARNAGLSGVNTYFNPLKKVFEYLNFYKLYSLKQIDEELIVEVLASITGALSDASKKNYRIAVINFFDFLDKQC"
  },
  {
    "reference": "MPHFLAKLDFKPLEYPLIEGDFCFHREFLSLKNPTKSCVYASFKDRIFLLQKIRRANDFLIKSEKATPLKREVLKQALRIYSQSFEVISHNLQENSKHASGKKTLDLGTFEDFIQKNQAPILIEIGFGSGRHLIELAKNNPTKTCLGIEIHTPSIAQALKQIELLDLKNLHILQGDGRLVLESMPNHRCEKIFVHFPVPWNEKKHRRVLSEKFLNEALRVLKPRGFLELRTDDSLYFEDSLKLALKNFQCEIEIKKNAQIPVVSKYEARWNKLKKDIYDLRIYSLEWNETPFDNHAFDFSFDTITISKKSVGTILKTKKIIQEGYFVHVCNIYENKGDFLVELSMGDFDWPVRLFVLLTENQIFYLNKSPLKTLNNHKAHLLLQNILSQKGIG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. We are looking for a protein with broad substrate specificity for guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-   methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine.2. The protein should be optimized for activity in tRNA modification pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The protein design should aim to mimic or enhance the function of natural proteins involved in tRNA methylation, potentially with improved properties such as stability, specificity or selectivity.4. The protein should have a high affinity for substrate, S-adenosyl-L-methionine. The designed protein sequence is ",
    "response#1": "MKRAVEKGIIEVHIYNIRDFSNNKHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQMSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGVLNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITKERK",
    "response#2": "MKRAVEKGIIEVHIYNIRDFSNNKHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQMSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGVLNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITKERK",
    "response#3": "MKRAVEKGIIEVHIYNIRDFSNNKHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQMSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGVLNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITKERE"
  },
  {
    "reference": "MNRVNDPLIFIRDIKPGLKNLNVVFIVLEIGRVTKTKDGHEVRSCKVADKTGSITISVWDEIGGLIQPGDIIRLTRGYASMWKGCLTLYTGRGGELQKIGEFCMVYSEVPNFSEPNPDYRGQQNKGAQSEQKNNSMNSNMGTGTFGPVGNGVHTGPESREHQFSHAGRSNGRGLINPQLQGTASNQTVMTTISNGRDPRRAFKR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: In the SOSS complex, acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines; The SOSS complex associates with DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability; Required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM- dependent signaling pathways.; Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint.2. The ideal protein would be one that plays a critical role in DNA repair, mitotic G2/M transition checkpoint, cellular response to DNA damage stimulus through single-stranded DNA binding within chromosome, telomeric region, SOSS complex.3. Incorporation of the OB domain in the designed protein should not interfere with its overall stability and folding.4. The protein should exhibit Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "QQHEEVCLGLFTLLLTEPPQAQRCYRDLTLVNRDGMNVVLMKINQILMEKFLKLQDVCRTQLVWLVRELVKSGVIGADGVLMTLMKQIAGGDISSKNLWLAENVLDILVDQREWVLKSGMLVAMSLYTYLRLIVDHGTTSLLPLRQREVDFCIGLLRERFMECFIIGRDLVRLLQNVARIPEMELVWRDLLHSPQTLSPQFTGILQLLTSRTSRKFLACRLTPDMETKLLFMTSRVRFGQQKRYQDWFQRQYLSTAESQSLRCDLIRYICGVVHPSNEVLSSDILPRWAIIGWLLTTCTSNVAASNAKLALFYDWLFFNPEKDSIMNIEPAILVMHHSMKPHPAITATLLDFMCRIIPHFFPPLEGQVRQGVFNSLNFIMEKRVLAHLAPLFDNPKLDRELRSMLRQHEEICLGLFTLILTEPAQAQKCYRDLALVSRDGMNIVLNKINQILMEKYLKLQDTCRTQLVWLVRELVKSGVLGADGVCMTFMKQIAGGGDVTAKNIWLAESVLDILTEQREWVLKSSILIAMAVYTYLRLIVDHHGTAQLQALRQKEVDFCISLLRERFMECLMIGRDLVRLLQNVARIPEFELLWKDIIHNPQALSPQFTGILQLLQSRTSRKFLACRLTPDMETKLLFMTSRVRFGQQKRYQDWFQRQYLSTPDSQSLRCDLIRYICGVVHPSNEVLSSDILPRWAIIGWLLTTCTSNVAASNAKLALFYDWLFFSPDKDSIMNIEPAILVMHHSMKPHPAITATLLDFMCRIIPNFYPPLEGHVRQGVFSSLNHIVEKRVLAHLAPLFDNPKLDKELRAMLR",
    "response#2": "QHEEICLGLFTLILTEPAQAQKCYRDLALVSRDGMNIVLNKINQILMEKYLKLQDTCRTQLVWLVRELVKSGVLGADGVCMTFMKQIAGGGDVTAKNIWLAESVLDILTEQREWVLKSSILIAMAVYTYLRLIVDHHGTAQLQALRQKEVDFCISLLRERFMECLMIGRDLVRLLQNVARIPEFELLWKDIIHNPQALSPQFTGILQLLQSRTSRKFLACRLTPDMETKLLFMTSRVRFGQQKRYQDWFQRQYLSTPDSQSLRCDLIRYICGVVHPSNEVLSSDILPRWAIIGWLLTTCTSNVAASNAKLALFYDWLFFSPDKDSIMNIEPAILVMHHSMKPHPAITATLLDFMCRIIPNFYPPLEGHVRQGVFSSLNHIVEKRVLAHLAPLFDNPKLDKELRAMLRVLQLQKGSDTEAQCEVMQEIVDQVLEEDFDSEQLSVLASCLQELFKAHFRGEVLPEEITEESLEESVGKPLYLIFRNLCQMQEDNSSFSLLLDLLSELYQKQPKIGYHLLYYLRASKAAAGKMNLYESFAQATQLGDLHTCLMMDMKACQEDDVRLLCHLTPSIYTEFPDETLRSGELLNMIVAVQHEEICLGLFTLILTEPAQAQKCYRDLALVSRDGMNIVLNKINQILMEKYLKLQDTCRTQLVWLVRELVKSGVLGADGVCMTFMKQIAGGGDVTAKNIWLAESVLDILTEQREWVLKSSILIAMAVYTYLRLIVDHHGTAQLQALRQKEVDFCISLLRERFMECLMIGRDLVRLLQNVARIPEFELLWKDIIHNPQALSPQFTGILQLLQSRTSRKFLACRLTPDMETKLLFMTSRVRFGQQKRYQDWFQRQYLSTPDSQSLRCDLIRYICGVVHPSNEVLSSDILPRWAIIGWLLTTCTSNVAASNAKLALFYDWLFFSPDKDSIMNIEPAILVMHHSMKPHPAITATLLDFMCRIIPNFYPPLEGHVRQGVFSSLNHIVEKRVLAHLAPLFDNPKLDKELRAMLR",
    "response#3": "QHEEICLGLFTLILTEPAQAQKCYRDLALVSRDGMNIVLNKINQILMEKYLKLQDTCRTQLVWLVRELVKSGVLGADGVCMTFMKQIAGGGDVTAKNIWLAESVLDILTEQREWVLKSSILIAMAVYTYLRLIVDHHGTAQLQALRQKEVDFCISLLRERFMECLMIGRDLVRLLQNVARIPEFELLWKDIIHNPQALSPQFTGILQLLQSRTSRKFLACRLTPDMETKLLFMTSRVRFGQQKRYQDWFQRQYLSTPDSQSLRCDLIRYICGVVHPSNEVLSSDILPRWAIIGWLLTTCTSNVAASNAKLALFYDWLFFSPDKDSIMNIEPAILVMHHSMKPHPAITATLLDFMCRIIPNFYPPLEGHVRQGVFSSLNHIVEKRVLAHLAPLFDNPKLDKELRAMLRQQHEEVCLGLFTLLLTEPPQAQRCYRDLTLVNRDGMNVVLMKINQILMEKFLKLQDVCRTQLVWLVRELVKSGVIGADGVLMTLMKQIAGGDISSKNLWLAENVLDILVDQREWVLKSGMLVAMSLYTYLRLIVDHGTTSLLPLRQREVDFCIGLLRERFMECFIIGRDLVRLLQNVARIPEMELVWRDLLHSPQTLSPQFTGILQLLTSRTSRKFLACRLTPDMETKLLFMTSRVRFGQQKRYQDWFQRQYLSTAESQSLRCDLIRYICGVVHPSNEVLSSDILPRWAIIGWLLTTCTSNVAASNAKLALFYDWLFFNPEKDSIMNIEPAILVMHHSMKPHPAITATLLDFMCRIIPHFFPPLEGQVRQGVFNSLNFIMEKRVLAHLAPLFDNPKLDRELRSMLR"
  },
  {
    "reference": "MARFIFVTGGVVSSLGKGLLSASLGALLQARGFKVRIRKFDPYLNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTGISARQSDNVTTGRIYRDIIMKERRGDYLGATVQVIPHVTNAIKEFALSDIDGLDFVICEIGGTVGDIESLPFVEAIRQLRNDLGRDQTVSVHTTLVPWIKAAGELKTKPTQHSVREISSLGVAPDVLLCRCEKEIPQSDREKIAQFCNVPPSAVIQALDARSIYDVPLQYHNEGLDDEVLKAFRIHDAPAPDLSRWTDVMDRVDHPEGEVTIGVVGKYVGLPDAYKSLNEALVHGGLANRSKVHIRWLDAEMFEGDDDNLAAELEPLHGILVPGGFGERGTEGKIASVRFARERKVPFFGICLGMQMACIEGARNLAGIAEASSTEFGETPEPVVGLITEWMSNEGLQKRAEDGDMGGTMRLGAYDARLSGNSHAAAAYGTTEISERHRHRYEVNIHYKDALEKGGLVFSGMSPDGQLPEIVERPDHPWFIGVQFHPELKSRPFEPHPLFAGFIAAAVKQSRLV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a high affinity for ATP, Mg(2+).2. The designed protein should have metal ion binding to facilitate glutamine metabolic process.3. The presence of Glutamine amidotransferase domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "QDAYLSIVEAFKHSGAVNNCEIDIQWISSDYLDSGNVNTLLEGVSGILVAPGFGARGIDGKLVAVTYARENRIPFMGICLGMQCAVIEYARNVCGWDQANSTEFDPETPYPIVDLMLEQKSIDEKGGTMRLGAYDCSLKQDSFSGSAYGKPVISERHRHRYEINNRYREELEANGLIVTGVNPDKDLVEIVEVKDHPWFVGVQFHPELKSTVKQPHPLFVDFLKK",
    "response#2": "EDAYISVNEALKHAGLEKNVDVNVRWVDSDRMDDAHEQRLREADGVVVPGGFGSRGTAGKIEAIRYARENDVPYLGLCLGFQLAVVEFARNVLDLEGAHSAEIDEETPHPVIDLLPEQYDLEDLGGTMRLGAHETDIEPGTLAHEVYGADSCTERHRHRYEVNPEYFDRFADTELVFSGQSGNRMEILELDDHPYFLGTQFHPEFRSRPTRASPPFVGLLN",
    "response#3": "VSVAVVGKYVANGDAYISIAEALKHGGIANNARVNLHWIDSENLRSEEIEARLHGMDAIVVAGGFGARGVEGKIQAVRYAREHRIPYLGLCYGMQMAVIEFARNVCRLRDAHTEECDAGSPHPVIHILPDQKNVVDKGASMRLGSYRCQLVAGSLAEKLYGSTVVHERHRHRYELNNEYRETLAKHGMVCSGLSPDYRLVEIVELPKHPFFIATQFHPEFKSRPNRAHPLFRGLVK"
  },
  {
    "reference": "MNFHQVLFLLLLIFLVDLSDYGVLADKTNDGYKNATKCNIYQGRWIYDNSSNPLYGTSTCPFIGLDCQKFGRPDKNYLHYRWQPTGCDIPRFNGRDFLTRFKGKKILFVGDSLSNNMWVSLSCMLHAAVPNAKYTFQLNKGLSTFTIPEYGISVNFLKNGFLVDLVSDKTRGLILKLDSISRGNQWLGSDVAIFNTFHWWSHTGRAKTWDYFQTGDKIVKEMNRMEAFKIALTTWSKWIDHNIDPSKTRVFYQGVSPVHLNGGEWGKPGKTCLGETVPVQGPSYPGRPNEGEAIVKSVIGRMAKPVELLDVTAMTEMRKDGHPSIYAGGGDRLNDCSHWCLPGVPDAWNQLLYTALLSH",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that May be involved in the specific O-acetylation of cell wall polymers .2. The protein should have Helical; Signal-anchor for type II membrane protein domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "LPRFNPEQFLDNMRNKWLAFIGDSISRNHVQSLLCILSQVEEVEDIFHDKEYKSRIWRFPSYNFTLSVIWSPFLVKAETFENGVPFSDIRVHLDKLDQKWTDQYINFDYVVISGGKWFLKTTIFHENNTVTGCHYCQGKNNMTELGYLYSYRKVLHLVLDFVAEPNHKAQVLFRTTTPDHFENGEWDSGGFCNRTMPFTEGSEGEMKSEDVSMRDIELEEFYKTTTTQQEGSNSNIVLLDTTSMSLLRPDGHPGPYRYPNPFAGLKNKELNQVQNDCLHWCLPGPIDSWNDLMVSWDATNFAGFFYDVNDDVSTESLTVTGLNGGNVIPEGGLVYETTIQMVDYEYSKPSVGWDQFPVLGFFAEEYIPINADKADKLAKLVLDSDDKYTIRTGEQLDLGEGYAIEAKQVDVDGEKVWLEFTKDGEFVDDEIISVVSGSDNTWEVELDDIQDEDDVVVLRVHVNQVFQGAVDSIAQIEGIWLIDYANAMKIESDDEFGDLDNVKINGATLTITNEDTFTLTRDDEVEIGQGMFFKVADTAASDLRYYPFVV",
    "response#2": "LPRFNATDFLVKLRGKSLMLVGDSMNRNQFESMLCVLREGLSDKSRMYEVHGHNITKGRGYFVFKFEDYNCTVEFVRSHFLVREGVRANAQGNTNPTLSIDRIDKSHAKWKRADILVFNTGHWWVHGKTARGKNYYKEGDYIYPKFDATEAYRRSLKTWAKWIDQNVNPKKQLVFYRGYSSAHFRGGEWDSGGSCNGEVEPVKKGSIIDSYPLKMKIVQEAIKEMQVPVILLNVTKLTNFRKDGHPSIYGKTNTDGKKVSTRRQDCSHWCLPGVPDVWNHLIYNN",
    "response#3": "LPRFNPEQFLDNMRNKWLAFIGDSISRNHVQSLLCILSQVEEVEDIFHDKEYKSRIWRFPSYNFTLSVIWSPFLVKAETFENGVPFSDIRVHLDKLDQKWTDQYINFDYVVISGGKWFLKTTIFHENNTVTGCHYCQGKNNMTELGYLYSYRKVLHLVLDFVAEPNHKAQVLFRTTTPDHFENGEWDSGGFCNRTMPFTEGSEGEMKSEDVSMRDIELEEFYKTTTTQQEGSNSNIVLLDTTSMSLLRPDGHPGPYRYPNPFAGLKNKELNQVQNDCLHWCLPGPIDSWNDLMVR"
  },
  {
    "reference": "MTKEKTLPLPRSECICPICQEILLEPVTLPCKHTLCNPCFQMTVEKASLCCPFCRKRVSTWARLHSRTRTLVNTELWERIQKQYPKECQRRASGQESDDLADELASCPAPLLCQPGEIRQEYEAEVSKIEAERLAQEEAERKASEDYIQKLLAEEAAQDRLHSEAVQREMEEQLKIDEELARTLSVDLDVSNASCSSVSSVTSKKVVVSKSSKNVKNKQRVSGDIERFLTPRAVAVVGIDESMNSDSSECFIVFDEGEDEMPELSPQCPSTSLIQERGVELIMPYLSDCYKVESNTTSQQDRCSERSQVCNGTYSSCSDSIDMEESMAIKKQSTTAGLFPERGYHSPENGMESNTMNCSTPKHLGMTGTLKRKCEDCSIDLEEKAGSCRNVKKKKLSLTEDCPVPSVHAGKLIELEENLYERRRQEEQDRLLALQLQRELDKELKQVNRGKGSPDEYELRTKRGLKLQECLKLQECQDSPLPLRKEIPVQDNSRNTQSEYSPDENKKPSRKNSVRSARVRQSRAVANTEGSSDGINVLKPINKQPTILDLFQRSAGK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of tp53bp1 and brca1; E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage; Acts with ube2n/ubc13 to amplify the rnf8-dependent histone ubiquitination; Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and ubiquitinates histone H2A and H2AX, leading to amplify the rnf8-dependent H2A ubiquitination and promoting the formation of 'Lys-63'-linked ubiquitin conjugates; Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively).2. The designed protein should be involved in chromatin organization, response to ionizing radiation, double-strand break repair, cellular response to DNA damage stimulus, histone H2A-K13 ubiquitination by localizing to nucleus, ubiquitin ligase complex and exhibiting K63-linked polyubiquitin modification-dependent protein binding, histone binding, ubiquitin-protein transferase activity, chromatin binding, ubiquitin binding.3. The protein should exhibit Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "MRVGRNSDWLRFYENSEITVGRGLDVTYQLLSPSCPLMISRLHCTFRQRDDGQWTVTDKKSLNGVWVNGSREVSIGRGAGVTYQLMSQSCPLMISRNHCVFKQNAKGKWTVTDNKSLNGVWLNRERAQITVGRGLDVTYQLLSPSCPLMISRLHCTFRQRDDGQWTVTDKKSLNGVWVNGSRIPAEEAHQLKLGDSIQLVTIGRGLGLTYQLVSKICPLMISRNHCVFKQNEEGQWTVKDNKSLNGVWLNKERLDPLKAYPIVEGDHIQLAYYHMRVGRNSDWLRFYENSEITVGRGLDVTYQLLSPSCPLMISRLHCTFRQRDDGQWTVTDKKSLNGVWVNGSREVTIGRGLGLTYQLVSKICPLMISRNHCVFKQNEEGQWTVKDNKSLNGVWLNKERAVTIGRGLGLTYQLVSKICPLMISRNHCVFKQNEEGQWTVKDNKSLNGVWLNKERLDPLKAYPIVEGDHIQLAYCDSKNWVTIGRGLGLTYQLVSKICPLMISRNHCVFKQNEEGQWTVKDNKSLNGVWLNKERLDPLKAYPIVEGDHIQLAWCGKKGFGAGD",
    "response#2": "MRVGRNSDWLRFYENSEITVGRGLDVTYQLLSPSCPLMISRLHCTFRQRDDGQWTVTDKKSLNGVWVNGSRRGNQKIEVTIGRGLGLTYQLVSKICPLMISRNHCVFKQNEEGQWTVKDNKSLNGVWLNKERITVGRGLDVTYQLLSPSCPLMISRLHCTFRQRDDGQWTVTDKKSLNGVWVNGSRIPAEEAHQLKLGDSIQLTNVTIGRGLGLTYQLVSKICPLMISRNHCVFKQNEEGQWTVKDNKSLNGVWLNKERLDPLKAYPIVEGDHIQLTEPQCGICREILLEPVTLPCSHTLCYPCFTSTVEKASLCCPYCRQTVVSKTRSLVLDNCIQELVDKFPSECKSRQESRK",
    "response#3": "MRVGRNSDWLRFYENSEITVGRGLDVTYQLLSPSCPLMISRLHCTFRQRDDGQWTVTDKKSLNGVWVNGSRVTIGRGLGLTYQLVSKICPLMISRNHCVFKQNEEGQWTVKDNKSLNGVWLNKERLDPLKAYPIVEGDHIQLYVTIGRGLGLTYQLVSKICPLMISRNHCVFKQNEEGQWTVKDNKSLNGVWLNKERLDPLKAYPIVEGDHIQLYAITVGRGLDVTYQLLSPSCPLMISRLHCTFRQRDDGQWTVTDKKSLNGVWVNGSRIPAEEAHQLKLGDSIQLYHAHPITVGRGLDVTYQLLSPSCPLMISRLHCTFRQRDDGQWTVTDKKSLNGVWVNGSRIPAEEAHQLKLGDSIQLYHAHPHGVTIGRGLGLTYQLVSKICPLMISRNHCVFKQNEEGQWTVKDNKSLNGVWLNKERLDPLKAYPIVEGDHIQLYHAHPHGTST"
  },
  {
    "reference": "MCHNKSSAPPTPAHISIQQNRTPGTDPVPYLHASSDTGSGDTIVLLTDIFGVTPFYRHLAAMLAEKGHDVVIPDVFHRVGHATDPGRDAALARRRQLDDRLAIEDIERTVAHTVDDQQTFGVLGFCLGGSFALLTAAAHPNQVTATYYAFPKGAPGAKVPVKPPLEAADAIDGPVLCHWGRDDYIDHEEIDQLEQILASAPGPSEIRWYDNAGHSFLAGLTEPNHPSTAAAHDSWQRTVEFFERYLTVGSAN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be optimized for carboxymethylenebutenolidase activity activity in order to improve aromatic compound catabolic process.2. A protein should be able to manipulate the activity of enzymes in the Aromatic compound metabolism pathway, either by activating or inhibiting their function.3. For general function, the protein need meet that Ring cleavage of cyclic ester dienelactone to produce maleylacetate.4. The protein should exhibit Polar residues compositional bias.5. The protein's active site must be carefully engineered to ensure that the amino acids involved in 2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-   2-enedioate + H(+) are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "CMSHTPLHGYFDPAPEVVAEVERVQRVARERVDAFDPELVIVFAPDHYNGFFYDVMPQFCIGVRATAIGDFNSAAGPLPVARDVALALADAALASDIDVAVSYRMQVDHGCAQALDVLTGGIDRYPVVPVFINSVAPPMASCRRARLLGDAIGRAAARMNRRVLLIGSGGMSHEPPVPEIAAADDVVAERLIAGRNPSPESRNARQSRTIAAAKAFAAGDSRLHPLNPAWDRALLELLERGEIAAADGLTNEAITRDAGKSAHEIRTWVAAFGALAASGPYAASIDYYRAIPEWIAGFSHGDNEVLSIKRVLQPKIEAEIALILERDLPRADTTFAELAEATGWVLPALEIVGSRVRDWNIRFVDTVADNASSGCFVLGGPVRRLAGVDLRRAAMRMTRNGEEVSSGSGAECLGHPLNAAVWLARTMAGLGEPLKAGDLILTGALGPMVGVAAGDRFEAEIEGIGQRY",
    "response#2": "CMSHTPLHGYFDPAPEVVAEVERVQRVARERVDAFDPELVIVFAPDHYNGFFYDVMPQFCIGVRATAIGDFNSAAGPLPVARDVALALADAALASDIDVAVSYRMQVDHGCAQALDVLTGGIDRYPVVPVFINSVAPPMASCRRARLLGDAIGRAAARMNRRVLLIGSGGMSHEPPVPEIAAADDVVAERLIAGRNPSPESRNARQSRTIAAAKAFAAGDSRLHPLNPAWDRALLELLERGEIAAADGLTNEAITRDAGKSAHEIRTWVAAFGALAASGPYAASIDYYRAIPEWIAGFHGLVLAEAVSIALARRIGREAAHHLVEQCCRRAVEQRRELRAVLGEEARVSAELSGDELDRLLDPAHYLGQARAWVERAVG",
    "response#3": "CMSHTPLHGYFDPAPEVVAEVERVQRVARERVDAFDPELVIVFAPDHYNGFFYDVMPQFCIGVRATAIGDFNSAAGPLPVARDVALALADAALASDIDVAVSYRMQVDHGCAQALDVLTGGIDRYPVVPVFINSVAPPMASCRRARLLGDAIGRAAARMNRRVLLIGSGGMSHEPPVPEIAAADDVVAERLIAGRNPSPESRNARQSRTIAAAKAFAAGDSRLHPLNPAWDRALLELLERGEIAAADGLTNEAITRDAGKSAHEIRTWVAAFGALAASGPYAASIDYYRAIPEWIAGFSHGDNEVLSIKRVLQPKIEAEIALILERDLPRADTTFAELAEATGWVLPALEIVGSRVRDWNIRFVDTVADNASSGCFVLGGPVRRLAGVDLRRAAMRMTRNGEEVSSGSGAECLGHPLNAAVWLARTMAGLGEPLKAGDLILTGALGPMVGVAAGDRFEAEIEGIGQH"
  },
  {
    "reference": "MKLTILTKRFRSSSPLRTSGSPTDTNPATQGQSTQTARKAETKGSRHHPKSPAVRKRRPYGRRRSRSPRDTTLRRGEGESARASAGSGERVAFISPGDVGTSTRSPPKGGQSRLRRLIQEARDPPIICLKGGPNQLKCLRYRIKASNSSDFESISTTWHWVHNKCTDRVGHARMLVRFISTEQRDRFLDKVVVPKSVSVILGAFDGS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be optimized for DNA binding activity in order to improve regulation of DNA replication.2. The protein should exhibit Polar residues compositional bias.3. The protein must exhibit the following characteristics: Plays a role in limiting the replication of viral DNA in keratinocytes; Recruits the host NCoR/SMRT complex to viral replication foci to mediate repression of both viral replication and transcription.. The designed protein sequence is ",
    "response#1": "PMILLKGTANSLKCWRYRKQNSSNCGFLFMSTVWNWVGDVSENHSRMLIAFKSPGQRDSFVKHNLFPKLCTYTYGSC",
    "response#2": "KFKEQFGLGFNELIRHFKSSKTVCLDWVVCVFGVYCTLAEGIKTLIQPQCDYAHIQVLSCQWGMTVLMLVRYKRAKNRETVAKGLSTLLNVPESHMLIEPPKLRSGPAALYWYKTAMSNCSDVYGETPEWIVRQTMVGL",
    "response#3": "KFKEQFGLGFNELIRHFKSSKTVCLDWVVCVFGVYCTLAEGIKTLIQPQCDYAHIQVLSCQWGMTVLMLVRYKRAKNRETVAKGLSTLLNVPESHMLIEPPKLRSGPAALYWYKTAMSNCSDVYGETPEWIVRQTMVGK"
  },
  {
    "reference": "MAESLQLVIISGMSGAGKTVAVQSFEDLGYFCIDNMPPALLPKFSELVEESGKIKKVALVIDLRSRAFYDEIMDMLANLDNTDFVSTRILFLDASNEELVSRYKETRRSHPLAMEGRVMDGVRKERELLAPLKDRASYVIDTSTLTPRELRESIFDKFETDQDETFHIEMLSFGFKYGLPIDADIVMDVRFLPNPYYIPELKKLTGLDKPVADYVMQQPATEAFYQQFLSMLESIMPGYEAEGKSSLTIAIGCTGGQHRSVALTQRIGEALAKHYKVHISHRDIEKRKETVNRS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be able to perform ATP binding, GTP binding under specific conditions.2. The designed protein should have a high specificity for ATP, GTP binding, with minimal off-target effects.3. For general function, the protein need meet that Displays ATPase and GTPase activities. The designed protein sequence is ",
    "response#1": "MQVNLQSFGFRYGLPLESDLVMDVRFLPNPYFVATLRPFSGLDQGVREYVMGHKETVVFLEHFRDMLELLLPSYRREGKSYLSVSIGCTGGRHRSVAIAEELYNYFRQRNVNIKITHRDIA",
    "response#2": "MSVTIISFGYKYGIPMDADLVFDVRFLPNPYYVRSLKERTGEETVVQDYILKWPVTQKFYKRFFDMMDFLLPEYSREGKSHFTIAIGCTGGKHRSVTTAIKLKEFLLSKGYHVVVEHKDR",
    "response#3": "MQVNLQSFGFRYGLPLESDLVMDVRFLPNPYFVATLRPFSGLDQGVREYVMGHKETVVFLEHFRDMLELLLPSYRREGKSYLSVSIGCTGGRHRSVAIAEELYNYFRQRNVNIKITHRDIG"
  },
  {
    "reference": "MSKTKIYINGKLIGTCENPEEFVEEMRKKRRSGEVSPEMNITHYPENHEIYIFTDPGRARRPLIIVEDGEPLLTEEHLEKLETGEMSWDDLIAAGIIEYLDAEEEENAYIAMSPDEINDEHTHLEIDPSTMLGICAGIIPFANHNSSPRNTMEAGMTKQALGLYASNYDLRTDTRAHLLHHPQVPIVKTRIIDVTGYDERPSGQNFVVAVMSYEGYNMEDALILNKASLERGLARSSFFRSYEAAERRYPGGQEDRFEIPEKGVRGYRSESDYRHLDEDGIINPEAEVSSGDVLIGKTSPPRFLEEIDEFGTVAERRRETSVTVRHGEKGIVDAVLLTETVEGSRLAKIRVREQRQPELGDKFASRHGQKGVVGLIVSQEDMPFTEDGIVPDLIVNPHAIPSRMSVGQVLEMLAGKAACMEGRRVDGTPFTGEDEDDIKEALRANGFETAGVESLYNGITGERIEAEIFIGVAYYQKLHHMTTDKVYARSRGPVQVLTRQPTEGRAREGGLRFGEMERDCLIAHGAALALKERLLDESDKYEALVCADCGMIAVYDKIRDKKYCPICEDSESFPVEVSYAFKLLLDELKSLCIFPKLILEDKA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have a fast turnover rate for a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +   RNA(n+1).2. The designed protein should have a binding site that is specific to the target Zn(2+) ligand.3. The protein can be targeted to cytoplasm and perform zinc ion binding. The designed protein sequence is ",
    "response#1": "LGMVLDAVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPEIIVNNEKRMLQEAVDSLFDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKQMALELFKPFVMKRLVDLNHAQNIKSAKRMVERYRPQVWDVLEEIITEHPVLLNRAPTLHRLGIQAFEPMLVEGKAIQLHPLVCGAFNADFDGDQMAVHLPLSPEAQAEARVLMLSSNNILKPSDGRPVTLPSQDMIIGLYHLI",
    "response#2": "MWMILDVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPDIIVRNEKRMLQEAVDALVDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPNLKMHQCGLPKEMALELFKPFVMKKLVDKAIASNIKSAKKMVERGREEVWDILDDVIKEHPVLLNRAPTLHRLGIQAFEPVLVEGRALQLHPLVCTAYNADFDGDQMAVHVPLSAEAQTEARLLMLASNNILNPKDGKPVVTPTQDMVIGLYYLL",
    "response#3": "MGMVLDCIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPEIIVNNEKRMLQEAVDALFDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKVMALELFKPFVMKRLVDLNHAQNIKSAERMVERATSGLSRYAMVWDVLEEVIKEHPVLLNRAPTLHRLGIQAFEPQLVEGKAIQIHPLVCAAFNADFDGDQMAVHLPLSAEAQAEARILMLSSNNILKPADGRPVTMPSQDMVFGIYYLL"
  },
  {
    "reference": "MAFLLLSSVLLVLVFLTAEVTLCTGSTECVARNFGHDSVVCECNSTYCDSVGSVSLPPLGQYSSYLSSMAGSRLKGAQAQVQVKSTGAGIRLTIVPYQKYQKIRGFGGAMTDAAAINILSLSAGAQDQLLRQYFSPDGIGYSVVRVPMASCDFSTRLYTYDDTPGDYNLDNFTLAPEDINMKCLERPRLPEGPAWGFLEEYAKYNLTFWALTTGNEPSAGRMTNYSFQALGFTPEEQRDWVALDLGPALHASSHPHTHILILDDNRLLLPHWAKVVLNDVHAGRYIHGVAVHWYMDFLVPAEISLGVTHHLYPEYYLFGTEACSGWNPLDRGVKLGSWDRAEQYAHDIIVDLNHYVVGWTDWNLALDQTGGPNWVKNFVDSPVIVDAQRDVFYKQPSFYSMAHFSKFLWEGSQRVGVSASQKTDLEYSAFIRPDGSVVLIILNRSSSEVQFEVWDPAVGYIPSTAPAHSLLTLAWNTH",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must have high specificity towards glucosylceramidase activity, glycosyltransferase activity. The designed protein sequence is ",
    "response#1": "LTGKTIVMPRRLVPDKQVDHAIQAFAKALPDHPGWRLRIFGDGPQMSRLRNLIQGLGLHDSVELLGPSQHMTEEWARASLTILPSQDGEAFPLVLLEAFAAGVPAVAYDIVTGPAEIIRHGEDGLLVPPNDVESLAEAISRLMGDEALLRSYGEKAE",
    "response#2": "LTGKTIVMPRRLVPDKQVDHAIQAFAKALPDHPGWRLRIFGDGPQMSRLRNLIQGLGLHDSVELLGPSQHMTEEWARASLTILPSQDGEAFPLVLLEAFAAGVPAVAYDIVTGPAEIIRHGEDGLLVPPNDVESLAEAISRLMGDEALLRSYGEKAE",
    "response#3": "LTGKTIVMPRRLVPDKQVDHAIQAFAKALPDHPGWRLRIFGDGPQMSRLRNLIQGLGLHDSVELLGPSQHMTEEWARASLTILPSQDGEAFPLVLLEAFAAGVPAVAYDIVTGPAEIIRHGEDGLLVPPNDVESLAEAISRLMGDEALLRSYGEKAR"
  },
  {
    "reference": "MPHNNQPSPGDDQLGPPKADFSAPPPYEANVGHGQQVALPAQMPPLALATPDPSQIPIQMQVQLPSQADLMNAQLPPEIHGKLPTYEEVQMEKSLNGELPPAFLTLPSSQQLPPPPNPLLPPNPLRDGAAAVRSAQPALTFIAIDASDPENSLSTTDNLLGTDIMFITAFIVAFLFNWIGFLMLTCFCHTIAARYGALSGFGLSLAKWTLIVKHSTDLASHENSWLWWLICAFGFLISIRALIQYVSIKRSWRLLSASAQERLLFFY",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be involved in vacuolar transport, wing disc development, positive regulation of Notch signaling pathway by localizing to ubiquitin ligase complex, Golgi apparatus, endoplasmic reticulum, membrane and exhibiting WW domain binding. The designed protein sequence is ",
    "response#1": "LASKELGKINRFKSPRDKMVCVLNASKVIFGLLKHTKLEQNGADSFIPVLIYCILKGQVRYLVSNVNYIERFRSPDFIRGEEEYYLSSLQAALNFIMNLL",
    "response#2": "MNRFAYVEARLSPNESFVSRDNRVKIYDGDQKTDFEDGEVVLTTHRLFWGRPGEIARAAVTLCLPLSYVISVSEETTASNFFGRKTRIIMHLHPPTSDKGPGPLDTSRATHIKLSGKNGLSVEFHSALRETLNARVWEILLTSR",
    "response#3": "VGLVNQAMTCYLNSLLQALFMTPEFRNALYRWEFDNDNEAKNIPYQLQKLFLNLQTSPKAAVETTDLTRSFGWDSTEAWQQHDIQELCRVMFDALEHKFKNTKQANLISNLYEGKMNDYVKCLECNTEKTREDTFLDIPLPVRPFGSSSAYGSIEEALRAFVQPETLDGNNQYLCEKCKKKCDAHKGLHFKSFPYILTLHLKRFDFDYQTMHRIKLNDRVTFPQTLNLNTFINRSGNSGEQNSQLNGTVDDCSTADSGSAMEDDNLSSGVVTTASSSQHENDLNDEDEGIDMSSSTSKSAKQGSGPYLYELFAIMIHSGSASGGHYYAYIKDFDNNEWFCFNDQNVTSITQEDIQRSFGGPNGSYYSSC"
  },
  {
    "reference": "MYPAHLLVLLAVCVSLLGASDIPPQPLNLYQFSNMIQCANRGRRPTKHYMDYGCYCGKGGSGTPVDELDRCCKVHDDCYGEAEKSQNCAPYWTWYTWKCGSDGPQCDDSETGCQRSVCECDAIAAKCFAKAPYNDANWDIDTETRCQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The active site active site of the designed protein must be optimized for substrate binding and specificity.2. The Ca(2+) binding site should be located in a region of the protein that is accessible to the ligand.3. The presence of Ca(2+) is necessary for the protein to function in the desired environment.4. The designed protein should be involved in phospholipid metabolic process, lipid catabolic process by localizing to extracellular region and exhibiting toxin activity, calcium ion binding, phospholipase A2 activity.5. Incorporate a signal peptide in the protein design.6. For general function, the protein need meet that PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides . The designed protein sequence is ",
    "response#1": "AVWQFRNMIKCTMPESDPLRDYNNYGCYCGLGGSGTPVDELDKCCQTHDRCYDQAKKLDTCKFLLDNPYTKSYSYKCSGSEIICSSKNKPCQAFICECDRKAAICFSKAPYNKEHKNLNTKKFCK",
    "response#2": "LLNLKAMVEAVTGRSAILSFVGYGCYCGLGGRGQPKDEVDWCCHAHDCCYQELFDQGCHPYVDHYDHTIENNTEIVCSDLNKTECDKQTCMCDKNMVLCLMNQTYREEYC",
    "response#3": "EMIQCTIRGSLTYLDYMDYGCYCGTGSRGTPVDELDRCCQTHDNCYAEAEEHPKCSSLVKSPYMNLYSYTCSGGTITCNADNDECGAFICNCDRTAALCFAKAPYNEENKEIDISKRC"
  },
  {
    "reference": "MNKKSIIILAAGAGTRMKSELPKVLHKISGKPMLYYSIKEALKLSDDVTVVLYHQFERVKSEMEKYFSNINFVIQDHINYPGTGGAVMGIIPKYEKVLVLNGDMPLIQANELHKFDIDATIVMSVLELESADGYGRVIIENGTVKKIVEQKDASIDELQITTANAGIYQFETKFLLENLPKLNNNNAQKEYYITDLVEMAINQNKVLKPLIVNEENFKGVNSKVELADAEVIHQNRIKKEFMKFGVIMRLPDTIYIEEGVQIEGESIIENGVTILGNSKIINSHIKTNSIVENSLVENSDIGPMARIRPYSKIISTHIGNFVETKKAVLTGVKAGHLSYLGDCFIDEGTNIGCGTITCNYDGVKKHQTIIGKNVFVGSDTQFVAPITVEDDVIIGAGTTVTNDIKKGELYVTRVKERVIDGYFYKHFENKKK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.2. The designed protein have MobA-like NTP transferase domains with specific sequence motifs that are crucial for its activity.3. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+), UDP-N-acetyl-alpha-D-glucosamine binding.4. The designed protein should have magnesium ion binding within cytoplasm to regulate cell wall organization, lipid A biosynthetic process, UDP-N-acetylglucosamine biosynthetic process, cell morphogenesis.5. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate. The designed protein sequence is ",
    "response#1": "VIILAAGQGSRMQSDLPKVLHQVAGKAMLSHVLDTATGLGASQLHVVVGHGSEQVQEALADREVNWVLQEQQLGTGHAVLQALPQVAEDAVVLVLYGDVPLITVDSLKPLVAAARSGPALLTATIEDPGGYGRIVRDASGDVLRIVEHKDADEATREIAEINSGMYVFDSRALAHALRRLSADNAQGEMYLTDALAILRGDGHRVGAHLAPDAREVQGVNDRVQLSEARRILNDRLLAEAMRAGVTLADPARLDVRGEVAHGTDVFIDVNVVLEGKVVLGDRVRLGAGCVIRNSEIGADTEVFPHCVIDGALIGPGCNIGPFARLRPTATLAGSVHIGNFVEVKNSRLGAGSKANHLAYLGDARLGERVNIGAGTIVANYDGANKHATLIEDDVHTGSNSVLVAPITVGAGATIAAGSTVTRAVPAGKLTVARARQRNIEGWVERKRAGTPAAEAAERVRQGRGTDE",
    "response#2": "VLAAGKGTRMKSSLPKVLQPLAGATLLERVIKNSXCLNPDRLLLIVGHQAERVQQQLQNHPGKPEFILQQPQNGTGHAIQQLMAPLAEFDGNLLVLNGDVPLLKQETLSQLLAMHCQSGAEATLLTAMLENPNGYGRVFSNNDGEVTAIVEDRDCDCEQRQNKLINAGIYCFKWSALKKVLPTLSSENDQGELYLTDTIAMLNNAKEAQEVKIVEDYLAVGVTLADPQRIDIRGSLRCGRDVVIDINAVFEGDVVLGDRVTIGPNCVIRDSELGEDTEVRACSIIEQSETGARCQIGPYARLRPESSLADEVRIGNFVEIKKSNLAFGSKVNHLTYVGDSEVGQRVNVGAGTITCNYDGAYKHKTVLGDDAFIGSGVQLVAPVRVGAGATIGAGTVVTKDAPEGKLTVGRARQVNKKGWANERRKAKAKKEAELAS",
    "response#3": "VIILAAGQGSRMQSDLPKVLHQVAGKAMLSHVLDTATGLGASQLHVVVGHGSEQVQEALADREVNWVLQEQQLGTGHAVLQALPQVAEDAVVLVLYGDVPLITVDSLKPLVAAARSGPALLTAVLVADPAGYGRILRDADSRITGIVEHKDATEAQRAVREINSGIYAFDAAVLRTALARLSSDNAQGEKYLTDVLGIARDDGRPVGAHLLDDVWQTEGVNDRVQLAALGAELNRRVLRRWMLAGVTMIDPSSTWIDATVTLAPDVLLYPNVYLEGSTTIAAGATIGPDTVLHDCTVGPSATVISSRCIGAQIAAGASVGPFSYLRPDAQLAENAKVGAFVEVKKSRIGKGSKVPHLSYVGDAEIGEETNIGAATVFVNYDGVNKHKTVVGNHVRIGSDNMLVAPVEIGDGAYTAAGSVITENVPPGAMAVARGRGVTLADPQRIDIRGSLRCGRDVVIDINAVFEGDVVLGDRVTIGPNCVIRDSELGEDTEVRACSIIEQSETGARCQIGPYARLRPESSLADEVRIGNFVEIKKSNLAFGSKVNHLTYVGDSEVGQRVNVGAGTITCNYDGAYKHKTVLGDDAFIGSGVQLVAPVRVGAGATIGAGTVVTKDAPEGKLTVGRARQRNIEGWVQRKRPGTAAAEAAERALAAHRAAGPDHAAPEHASPEHASPEHASPEHASPEQTAPGHPSPAHPAHPAHPAHPAHPAHPAHPSEQP"
  },
  {
    "reference": "MRVMGTQRNCLLSLRWGTMCIFWLMMVCNANKLWVTVYYGVPVWKDAKTTLFCASNAQAYNTEKHNVWATHACVPTDPNPQEMLLINVTENFNMWKNDMVDQMHEDIISLWDESLKPCVKLTPLCVTLNCTNARANKVTVNATTGNNETANANQTANATNSNTTTTELDMAAGELKNCSFNITTEVRDKQKQEYALFYKPDIEQIDGDNSSYRLINCNVSSIKQACPKVTFEPIPIHYCAPAGFAILKCKDKNYNGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEGEVKIRSENFTNNAKIIIVQLNESVIINCTRPNNNTRRSVHLGPGKTFYATGDVIGDIRQAHCNVSSKQWKEALQRVVVQLEKQFGNNKTIKFNSSSGGDVEITTHSFNCAGEFFYCNTSKLFNGTWFNGTWVNGTWYNNTLDSNDTENGTITLPCKIRQIVRMWQRVGQAMYAPPIQGVIHCQSNITGLLLTRDGDKNETETFRPTGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVEREKRAVGMGAIFLGFLGAAGSTMGAASITLTVQARQLLAGIVQQQSNLLKAIEAQQQLLRLTVWGIKQLQARVLALERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNKSYEEIWDNMTWLQWEREIDNYTVQIYTLIEEAQYQQEKNEKDLLELDNWASLWNWFTISKWLWYIRIFIMIVGGLIGLRIVFAVLSIINRVRQGYSPLSFQIPTPNPEGLDRLGRIEGEGGEQDRTR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Transmembrane protein gp41: Acts as a class I viral fusion protein.2. Ensure the presence of a signal peptide within the protein.3. The designed protein should contain one or more Retroviral envelope protein GP41-like domains that are essential for its function.4. The designed protein should have structural molecule activity within virion membrane, viral envelope, host cell plasma membrane, host cell endosome membrane to regulate viral protein processing.5. The protein design must feature the Di-leucine internalization motif, YXXL motif; contains endocytosis signal motif.6. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "MWVTVYYGVPVWREAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQELVLENVTENFNMWKNDMVDQMHEDVISLWDESLKPCVKLTPLCVTLNCSTILNCSTLNCSTTQSGNDTCNNNMCAEMKNCSFNTTTELRDKNRKEYALFYKLDIVPLERANNRTENNSSEEYRLINCNTSTTTQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGKGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENLTNNAKTIIVHLNESVEIVCTRPNNNTRKSIRIGPGQTFYATGAIIGDIRQAHCNISKSKWNSTLQRVGKKLAEHFPNRTIRFNSSSGGDLEITTHSFNCGGEFFYCNTSGLFNSTFNDTYAPNNTDTNETITIQCRIKQIINMWQQVGRAMYAPPIAGNITCNSSITGLLLVRDGGTKTDDNTTETFRPEGGNMRDNWRSELYKYKVVEIKPLGIAPTEAKRRVVQREKRXLGAAXVFLGFLGAAGSTMGAAAVTLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTAVPWNTSWSNKSLNEIWNNMTWMEWEKEIDNYTNLIYNLLEESQNQQEKNEQELLELDKWANLWNWFDISNWLWYIKIFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTRLPTQRGPDRPEGIEEEGGERDRDRSDPLVNGFLAIIW",
    "response#2": "MRVKGTQMNWPNLWKWGTLILGLVIICSASDNLWVTVYYGVPVWKEANTTLFCASDAKSYDREAHNVWATHACVPTDPNPQEVFLENVTEKFDMWKNYMVEQMHADIISLWDQSLKPCVKLTPLCVTLNCSDATSNGTNSNTTTNSTSSDSIMEEVKNCSFNVTTELRDKKEKIYALFYKLDVVQLDKKDNNSANYRLINCNTSAITQACPKVTFEPIPIHYCAPAGFAILKCNDKKFNGTGPCHNVSTVQCTHGIRPVVSTQLLLNGSLAEEEHVMIRSENLTNNVKTIIVQLNSTVNITCIRPSNNTRKSVRIGPGQAFYATXDVTGDIRRAHCNVSRTXWXNALRGVATQLGAYFVNKTIKFAXHSGGDLEITTHSFNCGGEFFYCNTSDLFSIWKNXSIIKTNDSSVITIPCRIKQIINMWQRVGQAMYAPPIQGVIRCESNITGLLLTRDGGTDNSTDNSTETFRXGGGNMKDNWRSELYKYKVVKIEPLGVAPTRAKRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAMERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKSQEEIWDNMTWMQWDKEISNYTNTIYRLLEDSQNQQERNEKDLLALDSWQNLWNWFNIANWLWYIKIFIIIVGGLIGLRIIFAVLSIVNRVRQGYSPLSLQT",
    "response#3": "MWVTVYYGVPVWREAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQELVLENVTENFNMWKNDMVDQMHEDVISLWDESLKPCVKLTPLCVTLNCSTILNCSTLNCSTTQSGNDTCNNNMCAEMKNCSFNTTTELRDKNRKEYALFYKLDIVPLERANNRTENNSSEEYRLINCNTSTTTQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGKGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENLTNNAKTIIVHLNESVEIVCTRPNNNTRKSIRIGPGQTFYATGAIIGDIRQAHCNISKSKWNSTLQRVGKKLAEHFPNRTIRFNSSSGGDLEITTHSFNCGGEFFYCNTSGLFNSTFNDTYAPNNTDTNETITIQCRIKQIINMWQQVGRAMYAPPIAGNITCNSSITGLLLVRDGGTKTDDNTTETFRPEGGNMRDNWRSELYKYKVVEIKPLGIAPTEAKRRVVQREKRAALGMLFLGVLSAAGSTMGAASITLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAIERYLKDQQLLGIWGCSGKHICTTTVPWNVSWSNKTYDEIWKNMTWMQWDREIDSYTGTIYRLLEDSQIQQEKNEEDLLALDSWNNLWNWFSITKWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTLTHHQREPDRPERIEEEGGEQGRDRSVRLVSGFLALAW"
  },
  {
    "reference": "MIVGLGNDLVDIRRIERMLVRYGERFIQRIFTDIERNRSENLKKNSSSYAKRFAAKEACAKALGTGIACGINWKDMGVVNLSSGKPIMQLTNHAQVQLQKLLPPHHDAIIHLSITDDFPWAQAFVIIEALPRG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.2. The protein must exhibit the following characteristics: Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein..3. The protein should be designed to catalyze the following reaction apo-[ACP] + CoA = adenosine 3',5'-bisphosphate + H(+) + holo-   [ACP].4. The designed protein should have a high specificity for Mg(2+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MIYGIGTDIIQIARVQGVMTRTNGRFAEKVLGPDELAKYHARKARSEKRGLAFLATRFAAKEAFSKAIGLGMRWPMTWRAMELMNLPSGEPTAVCHGELAAWLAERGLVVRVSVSDEHDYAVAFAIAEH",
    "response#2": "MIKGIGIDIIEIDRIGQAISKNNGFMDRIFTLNEQRLFEEKNNIMETIAGHFAAKEATAKALGTGIRNMKWKDIEILKDSLGKPYVELHNNAKTLADSMHIEQILISISHNKSNAVAQAA",
    "response#3": "MIYGIGTDIIQIARVQGVMTRTNGRFAEKVLGPDELAKYHARKARSEKRGLAFLATRFAAKEAFSKAIGLGMRWPMTWRAMELMNLPSGEPTAVCHGELAAWLAERGLVVRVSVSDEHDYAVAFAIAEE"
  },
  {
    "reference": "MSDKIQEEILGLVSRSNFKQCYAKLGQLQKQFPNALYFKILETYVKFKQSPGKFDYNKLLEEPYGLKGTTITGDTRSLEFLHNFFVELGKYDEALHVYERGNFKFPSYELSYHWFMKALEDSNYNQMSKASLQLAKYSDSGNLPKRAYYFWNAISILAVSRFQENTLSDPKKILLSRLARQSLLDLKPFQNVQEIIVYCLVLDELFPQSREISEEIVAITFANFDTSVNLYLKNFILKHTKLLNSPQKLFEVCSKLIEKGLDDYELITNLIDAAYKLSKSKDEVKQWIDENLGDSRNTRLARLKLDIMYTDSVSESSLSYYLSKYHNKPCCSIDLNHYSGHINIDMLKSIMSKYDPEDKDLIHHCNILELGLIGSDSINNYNKFKGTLEKKSVTDYSSCSTFLLEIVKDKCKKTNPELKDVLLCITILENYQAKDPHNFDTMCWLIVLYMYLGLVPDAYFHFINLKIKNVQTDSLDYMIFSRFSTLFPNKQSDFYSKTFHEHNNLYDTSLANLPRYIQVAFERNSYSKILGMLEMRDKLMKSYTRWTKTLENLQFSRLCNDKRGHLLQKLHEDWRSLEMTQSVSFSDNRDFSILDENFAQFLNRGKILEYANLNEESIFLTLIRELIIEALPNGEKTEQISALLKKLPSINLEELLNNNLTEVESASFLIFFEIYENNGKNLHDLISRLMKVPINAKQNWMVSHTYLTKMATLKTLDSLKRIKDKEIQKLIKNSLKELRSCCDDVFKGYSKALVQAYEELKKDECGNL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met; NatB acetylates TPM1 protein and regulates tropomyocin-actin interactions; MDM20 is required for mitochondrial inheritance during budding and together with TPM1, is essential for the integrity and assembly of actin cables.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm, NatB complex that is critical for cytoskeleton organization, N-terminal peptidyl-methionine acetylation, N-terminal protein amino acid acetylation, mitochondrion inheritance, regulation of actin cytoskeleton organization. The designed protein sequence is ",
    "response#1": "MEFPFDVDALLPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIVDELGKASAKAQHLPAPITSASRMQSNRHVIYVLKDTSARPAGKGAIIGFLKVGYKKLFVLDDREAHNEVEPLCILDFYIHESLQRHGHGQELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFPFDIHKLLVERITVLGPNLRPARRPFTDRVDFQRQITVILDEMGKASAKAQHLSAPITSTSRLQGSDHRLYILKDCSAKNAGKGTVIGFLKVGCKKLFVLDRHGAHNEMEPLCVLDFYIHEALQRHGYGKELFQYMLQQERLHPSQLAVDRPSEKLLSFLRKHYNLRSTIPQVNNFVVFEGFFQGRNVLKEEEEPPWPFNQSLVEQDDPFDVDALFPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIIDELGKASAKAQNLSAPITSASRMQSNRHVVYILKDGSARPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSPKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPAPSALFPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIIDELGKASAKAQNLSAPITSASRMQSNRHVVYILKDSSGRPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGF",
    "response#2": "MEFPFDVDALLPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIVDELGKASAKAQHLPAPITSASRMQSNRHVIYVLKDTSARPAGKGAIIGFLKVGYKKLFVLDDREAHNEVEPLCILDFYIHESLQRHGHGQELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFPFDVDALLPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIVDELGKASAKAQHLPAPITSASRMQSNRHVIYVLKDTSARPAGKGAIIGFLKVGYKKLFVLDDREAHNEVEPLCILDFYIHESLQRHGHGQELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHPPARKLPPKPFDVDALLPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIVDELGKASAKAQHLPAPITSASRMQSNRHVIYVLKDTSARPAGKGAIIGFLKVGYKKLFVLDDREAHNEVEPLCILDFYIHESLQRHGHGQELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHPPARKLPPKRCCTPFDIHKLLVERITVLGPNLRPARRPFTDRVDFQRQITVILDEMGKASAKAQHLSAPITSTSRLQGSDHRLYILKDCSAKNAGKGTVIGFLKVGCKKLFVLDRHGAHNEMEPLCVLDFYIHEALQRHGYGKELFQYMLQQERLHPSQLAVDRPSEKLLSFLRKHYNLRSTIPQVNNFVVFEGFFQGRNVLKEEEEPPWPFNQS",
    "response#3": "MEFPFDIHKLLVERITVLGPNLRPARRPFTDRVDFQRQITVILDEMGKASAKAQHLSAPITSTSRLQGSDHRLYILKDCSAKNAGKGTVIGFLKVGCKKLFVLDRHGAHNEMEPLCVLDFYIHEALQRHGYGKELFQYMLQQERLHPSQLAVDRPSEKLLSFLRKHYNLRSTIPQVNNFVVFEGFPFDVDALFPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIIDELGKASAKAQNLSAPITSASRMQSNRHVVYILKDGSARPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSPKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPAPSEFDIHKIFLEPITKLDSTLLPQRRPLVSSSEAEKQIMKVLDDIGKASAKAQRLPAPITSASRMQTNQHHLYILKDCTPKTAGRGLVIGFLKVGYKKLFVLDRKGSHIEAEPLCILDFYIHESLQRHGFGKELFGFMLRIEQVEPQHLAIDRPSEKFLSFLWKHYNLRAVIPQVNNFVIFEGFFRDRKAPGRKLPPKDFDVHKIFLEPITKLDSSLIPSRRPLIASSEAQKQIMTVIDEIGKASAKAQRLPAPITSASRMQTNKHHLYILKDCTPKTAGRGAVIGFLKVGCKKLFVLDQKGSHIEAEPLCILDFYIHETLQRHGFGKELFTFMLKNEQVDVHHLAIDRPSEKFLSFLRKHFNLWSTIPQVNNFVVFDGFFRDWKASVKKTPAK"
  },
  {
    "reference": "MENILLNKALLILEDGTKFYGNSIGAKGTSIGEVIFNTSITGYQEIITDPSYFNQIVAFTYPHIGNIGIEINNNESSKIQVKAVILYQTHNIISDFRNKLSFSRFLLLNNIIGISGIDTRKLTRLIRKSGTQYGYVSSEGTNINFKEIKSKIKIFLEKKNLDLVKLVTTKRKIIYKKKYIEKNFFYNIVAYDFGIKFSIIKNLINLGCRITLVPATTKSKYVISLNPDGILLSNGPGNPEIYKYAIRNIQCLLETKIPIFGICLGHQLLAIANGAKILKMKFGHHGSNHPVKDLETNKIIITSQNHDFAIDKSTLSKNIIITHISMFDQTLQGIKLINKPVFSFQGHPESSPGPKDALFLFKNFINLVKDYRLNKK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein with the reaction 2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP +   carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate, the active site must be optimized to ensure efficient catalysis.2. A protein with ATP binding is needed to enhance 'de novo' pyrimidine nucleobase biosynthetic process, glutamine metabolic process in a specific context. The designed protein sequence is ",
    "response#1": "MEALSNEAVKMMRLLGYEDVNRVNTTIGSEQEYFLIDKDFYKKRKDLLLTGRTLIGAPASKGQEMEDHYFGVIKPKVSAYMHDLDEELWKLGIPAKTKHNEVAPSQHELAPVFETANIAVDHNQLTMEVMKKVADKHNYACLLHEKPFEGVNGSGKHNNWSICTDTGINLLDPGKNPGENIPFLVFLMSVIAAVDEYAPILRLSVASAGNDHRLGGNEAPPAIISIFVGDELAEVLKAVEAGEAYKAAGKSQMTWEQQYFTFTKDNTDRNRTSPFAFTGNKFESS",
    "response#2": "DRDSLLRSMEALSNEAVKMMRLLGYEDVNRVNTTIGSEQEYFLIDKDFYKKRKDLLLTGRTLIGAPASKGQEMEDHYFGVIKPKVSAYMHDLDEELWKLGIPAKTKHNEVAPSQHELAPVFETANIAVDHNQLTMEVMKKVADKHNYACLLHEKPFEGVNGSGKHNNWSICTDTGINLLDPGKNPGENIPFLVFLMSVIAAVDEYAPILRLSVASAGNDHRLGGNEAPPAIISIFVGDELAEVLKAVEAGEAYKAAGKSQMTWEQQYFTFTKDNTDRNRTSPFAFTGNKFESDGGHSSVANGKYGPQHMQLQKEVATLNAKLSAYSGDELKEKVKEVLKETLLAHKRVLFNGNGYTDEWVEEAAKRGLPNLKALPDCMPYWISDESIDLFTRHGIFTKEEIYSRYEILLENYSKSIHIESLTMQEMIRKDRL",
    "response#3": "AYDYGVKQNILRMLVDRGCDVTVVPAKTPASEVLAMNPDGVFLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEKGNVMITSQNHGFAADEATLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIERDPRFIAEEAEKYQTEQGYTSYYGPELEFFMFDKVKLDVSAPQSGTGYKIYAREAPWSDSGTFVIRFKEGYYPAPPVDQLMDVRVEIVDTLVKYFGYTIEATHHEVATAGQGEIDFRFSTLVDTADKVQTLKYVAKNIAAKHGLVVTFMPKPIYGDNGTGMHTHLSLWTKDGKKNLMYDPNDEYAEISQFGRYVIGGLLTHARALSAIVSPTVNSYRRLIPGFEAPVYIAWSKGNRSAVIRVPAYYRGMEKAKRIEYRPPDPSTNPYLAFAALLMAALDGVNKKIDPGDPVDENIYHLTPEKRRQLGIKELPRSLNEALDELESDKEFLKPVFNSSILDTYIDLKRDEARSLQGYPHPMELYYPA"
  },
  {
    "reference": "MAVKKRGLGRGLDALLSGPSVSALEEQAVKIDQKELQHLPVELVQRGKYQPRRDMDPEALEELAHSIRNHGVMQPIVVRPIGDNRYEIIAGERRWRATQQAGLDTIPAMVREVPDEAAIAMALIENIQREDLNPLEEAMALQRLQQEFELTQQQVADAVGKSRVTVANLLRLITLPDAIKTMLAHGDLEMGHARALLGLDENRQEEGARHVVARGLTVRQTEALVRQWLSDKPDPVEPSKPDPDIARLEQRLAERLGSAVQIRHGNKGKGQLVIRYNSLDELQGVLAHIR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Binds to the DNA origin of replication .; Localize to both poles of the predivisional cell following completion of DNA replication2. The protein must have high specificity towards DNA binding. The designed protein sequence is ",
    "response#1": "MLVQIINIDKIRPNENQPRKNFEYDKIKELADSILNNGLLQPIIVDNNFQIIAGERRWRACKLAKVLEIPVIIKNLDARESMEIALIENIQRTDLTVMEEARGFKYLIENFNYTAEKLAERLGKSRSHIANLLRLNNLPQSIQDKVNENILSMGQARCLINHEL",
    "response#2": "VQIINIDKIRPNENQPRKNFEYDKIKELADSILNNGLLQPIIVDNNFQIIAGERRWRACKLAKVLEIPVIIKNLDARESMEIALIENIQRTDLTVMEEARGFKYLIENFNYTAEKLAERLGKSRSHIANLLRLNNLPQSIQDKVNENILSMGQARCLINHEL",
    "response#3": "VQIINIDKIRPNENQPRKNFEYDKIKELADSILNNGLLQPIIVDNNFQIIAGERRWRACKLAKVLEIPVIIKNLDARESMEIALIENIQRTDLTVMEEARGFKYLIENFNYTAEKLAERLGKSRSHIANLLRLNNLPQSIQDKVNENILSMGQARCLINHEP"
  },
  {
    "reference": "MAASVANCCFSVLNASVKIQSSSISSPWCFVSASSLTPRASSNIKRKSSRSDSPSPILNPEKNYPGRVRDESSNPPQKMAFKAFGSPKKEKKESLSDFSRDQQTDPAKIHDASFLNAVVKVYCTHTAPDYSLPWQKQRQFTSTGSAFMIGDGKLLTNAHCVEHDTQVKVKRRGDDRKYVAKVLVRGVDCDIALLSVESEDFWKGAEPLRLGHLPRLQDSVTVVGYPLGGDTISVTKGVVSRIEVTSYAHGSSDLLGIQIDAAINPGNSGGPAFNDQGECIGVAFQVYRSEETENIGYVIPTTVVSHFLTDYERNGKYTGYPCLGVLLQKLENPALRECLKVPTNEGVLVRRVEPTSDASKVLKEGDVIVSFDDLHVGCEGTVPFRSSERIAFRYLISQKFAGDIAEIGIIRAGEHKKVQVVLRPRVHLVPYHIDGGQPSYIIVAGLVFTPLSEPLIEEECEDTIGLKLLTKARYSVARFRGEQIVILSQVLANEVNIGYEDMNNQQVLKFNGIPIRNIHHLAHLIDMCKDKYLVFEFEDNYVAVLEREASNSASLCILKDYGIPSERSADLLEPYVDPIDDTQALDQGIGDSPVSNLEIGFDGLVWA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should prioritize the formation of helix-strand-strand-strand-strand-helix-strand-strand-turn-strand-helix-strand-strand-strand-turn elements.2. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.3. The protein should be optimized for stability and expression in chloroplast, cytosol where it will participate in photosystem II repair, protein catabolic process. The designed protein sequence is ",
    "response#1": "RGAAWKQVPLPTESVLFDIDFSQKDPNHGWLVGTRGLVLETRDGGETWEPRAFEDVEREEELNYRFSNVSFSGDEAWVIGKPPVMLRSTDGGKNWSRILLSPKLPGEPLLVTALGPNCAEMVTSSGAIYVTENGGINWKALVRETIDATLNRTISSGITGASYFTGSIVSVSRDVHGNYIAIPSRGNFFLTWVPGSDFWTPHARSTSRRISAIGFIQNDATKGIWETIRGGGLGFTKPNVNLNSTETIAFDMVDSKTGGYGILDVAFQDDRHVWAAVGGGSMYRSDDGGKTWRRDPLVSKVGANLYKIKFFGSQRGFVLGADGVLLKCS",
    "response#2": "YWTSKPEPSTREQFLQYVHDITFDPDTAHKYLRLQEENRKVTNTTPWEHPYPDLPSRFLHWRQVLSQQSLYLHRYYFEVEIFGAGTYVGLTCKGIDQKGEERSSCISGNNFSWSLQWNGKEFTAWYSDMETPLKAGPFWRLGVYIDFPGGILSFYGVEYDSMTLVHKFACKFSEPVYAAFWLSKKENAIRIVDLGEEPC",
    "response#3": "PLCADPFSPEIEQLCQALVREAQDDKTLCFENFTKPCPQLTKKQVELCKGFDYGDKTLKLPCGPLPWPAGLPEPGYVPKTNPLHGRWITVSGGQAAFIKEAIKAGMLGAAESHKIMADTDHHQTGGMYLRINQNGDVCTVDASVAKFARAKRTWKSGHYFYEPLVSGGNLLGVWVLPEEYRKIGFFWEMESGRCFRIERRAFERNGLMIMRQATEVAGKISFVFYVKVSNDPESKPIPLQSRDYTALAGLDNVPDSLGNPYTCEAKDLDYPIKRDTWLDKNQEEMLKQRSIVGTAF"
  },
  {
    "reference": "MRIITAVQASELDQKSMEDYGISGIDLMGAAGKEIAENVKTIVAEIHDPRIIIICGKGNNGGDGFATAAWLDKYSVQIFTSVDKELIKSDSAHFHNVCVEKDITIHYSENPPIDFECDLVIDALLGTGCKGELKDDIVKWTMWMNALHCIVLAVDCPTGINGNNGMAASHSVKAGKTITMGFPKLGLIMKQGQDHTGKIKTVDIGFPNIIDDLSGLKWSRFEESDISDLLSKIETDTYKHQQGKVLLIAGSKGMTGAAVLSTFGSLRSGAGLTITCAPESIEDIYEKTIIEGMTIGCPDDGNGFLISDSFEIIKDKIQWCDAIVIGPGLGRSPNTIKMVKKVVLNANKPLIIDADALCFFQRKLENFNKIKTPFIITPHEGEFCNLLKITREEYIKSFPDIVEKFMINFPGVLVLKNAPTITYYNDKAIVNSSGNPGMATAGMGDILAGILGTFVSQGMDCFQAAQAGVYIHGLAADNKVIEKGQRGLIASDLLDELPKVLHAFE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein with NADHX epimerase activity, metal ion binding, ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding is needed to enhance nicotinamide nucleotide metabolic process in a specific context.2. The protein should have a fast turnover rate for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.3. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.4. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration5. The protein contains novel YjeF N-terminal domains that confer a unique function or activity.6. The designed protein should have a binding site that is specific to the target K(+), AMP, (6S)-NADPHX ligand. The designed protein sequence is ",
    "response#1": "MREIDRLTTERYATPSLLLMEAAATATARSIAARFPGGLADKTARVICGRGNNGGDGAALARALWLAGVRTDVVLIGRVEEMKNDARTNFEIVRHLASFEAGSRQSRPPLTFVECESITDWEDIARPRHTYQIIVDALFGTGLSRPLEGIALMVVEHLALIRSARERAHEADPLIVSMDIPSGLNADGSEVTGEVVHADMTVTFTAPKPANVLPPASHYNGQLIIAGIGSPELTGRDDVRVLPPRRQRGSTKFTSGHVLVAGGSRDLMGAPRMAAHASMRAGAGYVTACVPASLQAIIATAAPAELMTRGLPDDGGALLGDGAGAVLELAARGGALVLGPGLGRSEGAGAFARELARSAELPLVLDADGLNAHAGRASELAARRAATVLTPHAGELARLLELEHEEVDRERLRYARAASERTHAVVVLKGDDSLIAEPGGQVAVSPGGSPALATAGTGDVLSGVIAALLAQGMEAFAAAAAGVWLHVLAGREAARRQGAAEGVLAGDVIAALPAALLPVRTASAHKGTAGRVWIVGGHPGTVGAPALAGLGALRAGAGLVTVHSAADVPLVTPELMAQRHADLRTFLTSTGLTGTGARPDAVAIGMGLGPEAPALAREVLDWEIPTVLDADALQPDLCGYGHDRCVWTPHPGEAARVLGVPTPEVTRDPLSAARALQDRLGGVVVLKGGPSVVARAGDLSVARGGHPGMASAGMGDTLCGILAALLAQGLDPWDAARAGVRLHARAGEIAAVRFGYGLGATDVAHDLGRAALLPVRTASAHKGTAGRVWIVGGHPGTVGAPALAGLGALRAGAGLVTVHSAADVPLVTPELMAQRHADLRTFLTSTGLTGTGARPDAVAIGMGLGPEAPALAREVLDWEIPTVLDADALQPDLCGYGHDRCVWTPHPGEAARVLGVPTPEVTRDPLSAARALQDRLGGVVVLKGGPSVVARAGDLSVARGGHPGMASAGMGDTLCGILAALLAQGLDPWDAARAGVRLHARAGEIAAVRFGYGLGATDVAHDLGRA",
    "response#2": "VLVVAGSRGMTGAASLVGEAALRSGSGLITVAVPETLHDILEAKLTEVMTVPLPDTGNGNLSLGARQRILSLLEGVNVLALGPGLSTEPEVATMVRELLPLVRVPCVLDADGLNALAGNVEILRKIQTPVVITPHPGEMARLMGVTTREIQEDRLSVASKAAASWNVVALLKGARTVVAAPGGAIYINPTGNPGMATAGSGDVLTGIVASLISQGLDPALAASAGAYIHGMAGDMGAREKGMIGLTAGDIITYLEAHKNQFGRIGIVAGSRGFAGAATMCAWGALRAGAGLVEVFVPENIYEVVAASAPFESMVKPVTSYRDLLDEKIDVWGIGPGLGKENAREIIDLVQRTKNAMVVDADALNLISREVSILKNCRGPRLLTPHPGEMKRLAGDRKISRAKLAKNFTAEFPVTLLLKGSRTVVAENGKRISYNTTGNPGMATGGMGDVLTGVCASLIAQNLSPFDAARVGAWVCGRAAELVV",
    "response#3": "MREIDRLTTERYATPSLLLMEAAATATARSIAARFPGGLADKTARVICGRGNNGGDGAALARALWLAGVRTDVVLIGRVEEMKNDARTNFEIVRHLASFEAGSRQSRPPLTFVECESITDWEDIARPRHTYQIIVDALFGTGLSRPLEGIALMVVEHLALIRSARERAHEADPLIVSMDIPSGLNADGSEVTGEVVHADMTVTFTAPKPANVLPPASHYNGQLIIAGIGSCAATLRSLLPRRQFEAHKNQFGRIGIVAGSRGFAGAATMCAWGALRAGAGLVEVFVPENIYEVVAASAPFESMVKPVTSYRDLLDEKIDVWGIGPGLGKENAREIIDLVQRTKNAMVVDADALNLISREVSILKNCRGPRLLTPHPGEMKRLAGDRKISRAKLAKNFTAEFPVTLLLKGSRTVVAENGKRISYNTTGNPGMATGGMGDVLTGVCASLIAQNLSPFDAARVGAWVCGRAAELAIFNCGESEQSLLPRDVLTHLGRAFREDDATLGALLPVRTASAHKGTAGRVWIVGGHPGTVGAPALAGLGALRAGAGLVTVHSAADVPLVTPELMAQRHADLRTFLTSTGLTGTGARPDAVAIGMGLGPEAPALAREVLDWEIPTVLDADALQPDLCGYGHDRCVWTPHPGEAARVLGVPTPEVTRDPLSAARALQDRLGGVVVLKGGPSVVARAGDLSVARGGHPGMASAGMGDTLCGILAALLAQGLDPWDAARAGVRLHARAGEIAAVRFGYGLGATDVAHDLGRAWADLA"
  },
  {
    "reference": "MRKPIIAGNWKMNKTVKEAKDFVNNLPTLPDEKEVESVICAPTIQLDALISLVNDGKAKGLKIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRELFHETDEDVNKKAHAVFNHGMTPIICVGETDEERENGKANEIVSNQVEKALEGLSEEQLKEVVIAYEPIWAIGTGKSATSDDANEMCAHVRKTVAKVASQDVADATRIQYGGSVKPNNVKEYMAQSDIDGALVGGASLKVEDFVQLLEGAK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate binding.2. The ideal protein would be one that plays a critical role in gluconeogenesis through triose-phosphate isomerase activity within cytoplasm.3. The designed protein is the optimization of the Electrophile to achieve high catalytic efficiency for D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate.4. A protein that is capable of catalyzing reactions within the Carbohydrate degradation pathway with high efficiency and specificity.5. The protein must exhibit the following characteristics: Involved in the gluconeogenesis. The designed protein sequence is ",
    "response#1": "MNNEKLNTV",
    "response#2": "MGGFLNIFSKKKK",
    "response#3": "MTMPLSAPMQKPTV"
  },
  {
    "reference": "MAKYIERIRNVIPGMRRRDSNVPHQGDVAVDMGEADFGKKENQKMSIYNPLDPVPKYDFYAKDTWAGRIKKSRPSLDVLRNPPDDITPTPDEEDGRPKVKLVRFGWVLGVWIRCMLNIWGVIMYLRLPWVTSQAGLVLTFVIIFMSVTITTITATSVSAISTNGKVYSGGTYFMISRSLGPELGAPIGLLFAFACSIACALHTVGFSETVRDMLKDFKSQMVDDVNDVRIIGAITVTICLLITFAGMAWEAKAQILFFIAIILSLLNYFVGTVIPPTQEKEAVGFFGYHSDIFVSNLLPSFRGPSGSFLVVFAIFFPACTGILSGVNICGDLKDPTGGIPKGTLLSILCTTLSYLLIAATCAATVVRDASGNKNDSLAISNYTIHCSGLGCKYGWNFDSCEQYRNCTQGLANNLRILTVVSGWGILITIGTFAATLSSALGFLVSAPKVFQLLCKDKIYPYIGFFGKGYGKNKEPLRAYVLTFIIAMCFILIGNLNTIAPLISNFFLASYGLINFSCFHASITKSPGWRPQYRYYSPWLSLFCCCLSFTLMFLFTWWAALVTFSVVLFLIGYVNYKKTYVNWGSSYQAAFYNMALNFSMNLTTVPDHVKNFRPQCLVLSGPPNTRPALVDFVGTFTKNVSLMICGNITIEGEQSDFPEHSSDLVVEWLTKRKVRSFYTTFAAESLQDGAHNLMLASGLGKLKPNTLVLGYKTNWQECKPESLQDYINTINDAFDSNYGVALLRMMDKLDITDDMYSAEDGLSAIDNPA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have at least one Amino acid permease/ SLC12A, SLC12A transporter C-terminal, Amino acid permease N-terminal domain.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. The designed protein should have cation:chloride symporter activity within plasma membrane, apical plasma membrane to regulate chloride transport, sodium ion transport.4. The protein should exhibit Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "MTWFQTLIHLLKGNIGTGLLGLPLAVKNAGLLLGPLSLLVIGIVAVHCMGILVKCAHHLCRRLNKPFLDYGDTVMYGLECSPSTWIRNHSHWGRRIVDFFLVVTQLGFCCVYFVFLADNFKQVIEAANGTTTNCNNNETVILTPTMDSRLYMLTFLPFLVLLSFIRNLRILSIFSLLANISMFVSLIMIYQFIVQRIPDPSHLPLVAPWKTYPLFFGTAIFAFEGIGVVLPLENKMKDSQKFPLILYLGMAIITVLYISLGSLGYLQFGADIKGSITLNLPNCWLYQSVKLLYSIGIFFTYALQFYVAAEIIIPAIVSRVPERFELVVDLSARTAMVCVTCVLAVLIPRLDLVISLVGSVSSSALALIIPPLLEVTTYYGEGISPLTITKDALISILGFVGFVVGTYLYTKQ",
    "response#2": "MKQAGFPLGILLLFWVSYVTDFSLVLLIKGGALSGTDTYQSLVNKTFGFPGYLLLSVLQFLYPFIAMISYNIIAGDTLSKVFQRIPGVDPENVFIGRHFIIGLSTVTFTLPLSLYRNIAKLGKVSLISTGLTTLILGIVMARAISLGPHIPKTEDAWVFAKPNAIQAVGVMSFAFICHHNSFLVYSSLEEPTVAKWSRLIHMSIVISVFICIFFATCGYLTFTGFTQGDLFENYCRNDDLVTFGRFCYGVTVILTYPMECFVTREVIANVFFGGNLSSVFHIVVTVMVITVATLVSLLIDCLGIVLELNGVLCATPLIFIIPSACYLKLSEEPRTHSDKIMSCVMLPIGAVVMVFGFVMAGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFISLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSAACKLNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQ",
    "response#3": "MTWFQTLIHLLKGNIGTGLLGLPLAVKNAGLLLGPLSLLVIGIVAVHCMGILVKCAHHLCRRLNKPFLDYGDTVMYGLECSPSTWIRNHSHWGRRIVDFFLVVTQLGFCCVYFVFLADNFKQVIEAANGTTTNCNNNETVILTPTMDSRLYMLTFLPFLVLLSFIRNLRILSIFSLLANISMFVSLIMIYQFIVQRIPDPSHLPLVAPWKTYPLFFGTAIFAFEGIGVVLPLENKMKDSQKFPLILYLGMAIITVLYISLGSLGYLQFGADIKGSITLNLPNCWLYQSVKLLYSIGIFFTYALQFYVAAEIIIPAIVSRVPERFELVVDLSARTAMVCVTCVLAVLIPRLDLVISLVGSVSSSALALIIPPLLEVTTYYGEGISPLTITKDALISILGFVGFVVGTYLYARFS"
  },
  {
    "reference": "MDTIEKKSVATIRTLSIDAIEKANSGHPGMPMGAAPMAYTLWTKFMNVSPANPGWFNRDRFVLSAGHGSALLYSMLHLSGFDLSIEDLKGFRQWGSKTPGHPEFGHTAGVDATTGPLGQGIAMAVGMAIAERHLAETYNRDSFNVVDHYTYSICGDGDLMEGISSEAASLAGHLQLGRLIVLYDSNDISLDGDLDRSFSENVKQRFEAMNWEVLYVEDGNNIEELTAAIEKARQNEKKPTLIEVKTTIGFGSPNRAGTSGVHGAPLGKEESKLTKEAYAWTYEEDFYVPSEVYEHFAVAVKESGEKKEQEWNAQFAKYKEVYPELAEQLELAIKGELPKDWDQEVPVYEKGSSLASRASSGEVLNGLAKKIPFFVGGSADLAGSNKTTIKNAGDFTAVDYSGKNFWFGVREFAMGAALNGMALHGGLRVFGGTFFVFSDYLRPAIRLAALMGLPVTYVFTHDSIAVGEDGPTHEPVEQLASLRAMPNLSLIRPADGNETAAAWKLAVQSTDHPTALVLTRQNLPTIDQTSEEALAGVEKGAYVVSKSKNETPDALLIASGSEVGLAIEAQAELAKENIDVSVVSMPSMDRFEKQSDEYKNEVLPADVKKRLAIEMGSSFGWGKYTGLEGDVLGIDRFGASAPGETIINEYGFSVPNVVNRVKALINK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein requires thiamine diphosphate to activate a critical catalytic site.2. The protein should be able to bind thiamine diphosphate ligand in a variety of conditions3. A protein that can perform transketolase activity, metal ion binding in cytosol to support pentose-phosphate shunt.4. For general function, the protein need meet that Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.5. The protein's Proton donor must be carefully engineered to ensure that the amino acids involved in D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate =   aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "PSWSQLMSDAVLELAREDKRIVAITPAMPVGSKLTKFQQELPDQFFDVGIAEQHAVTMAGGLAANGMKPYVAIYSTFLQRAYDQLLHDIDRQQLDVVFGIDRSGLVGADGETHQGVFDIPFLSHLPNMTVMMPKDEDEAAKMVYSAFNTFGGPIAIRYPRGHHVALATRAASGAVLSALGPKLPELWGGSADLAGSNNTTIKGADSFGPPSISTKEYTAHWYGRTLHFGVREHAMGAILSGIVLHGPTRAYGGTFLQFSDYMRPAVRLAALMDIDTIYVWTHDSIGLGEDGPTHQPIEHLSALRAIPRLSVVRPADANETAYAWRTILARRNGSGPVGLILTRQGVPPELPLPQVGGYVLSDAGGLQPGEEPDVILIATGSEVQLAVAAQTLLADNDILARVVSMPCLEWFEAQPYEYRDAVLPPTVSARVAVEAGVAQCWHQLVGDTGEIVSIEHYGESADHKTLFREYGFTPEAVADAVRTFLIEKCAVTGGHIGANLVRLYLITPDTAEAPGVLAEQVAQAISGGARLVQYRQKGRDRQLRRAQAAAVLGVCRVAGVPFIVNDDVELAADIGADGVHLGRDDGDPITARRRLGDDAIIGVSCYDDMALARAAERAGASYVAFGSFFPSATKPHAVRASVERLSAARDSLRIPAVAIGGITPQNGGLLIAAGARMLAVVTPEAARA",
    "response#2": "PTYTQVFGETLCELAEKDPRITAITAAMPSGTGLNLFAQKFPDRFFDVGIAEQHAVTFSAALAFGGMKPVVSIYSTFYQRAYDQVLHDVCLPHANVVMAIDRAGVVGDDGPTHHGVFDISFLRVIPNLVFMAPKDENELRHMLYTSLQLDGPVALRYPRSVGQGLATRKTSQQVLTNMVQVLPELIGGSADLTPSNLTRWEGAVDFQPPITQLGNYAGRYIRYGVREHGMGAIMNGISAFGANYKPYGGTFLNFVSYAAGAVRLAALSGNPVIWVATHDSIGLGEDGPTHQPIETLAHLRAIPNMHVWRPADGNETSAAYYSAIKSGRTPSVVALSRQNLPGQPELTALATRKTSQQVLTNMVQVLPELIGGSADLTPSNLTRWEGAVDFQPPITQLGNYAGRYIRYGVREHGMGAIMNGISAFGANYKPYGGTFLNFVSYAAGAVRLAALSGNPVIWVATHDSIGLGEDGPTHQPIETLAHLRAIPNMHVWRPADGNETSAAYYSAIKSGRTPSVVALSRQNLPEGVAEQDIATRKAGSDVLQPISQYLPLSVSGSADLHGSTLNYIKEGRDFTPACPTGRNIKFGIREHAMGAMMNGIAYHGLFKVSGATFLVFSDYLRPAIRLAALSHLPVVYIFTHDSVGVGEDGPTHQPVETVSGLRMIPNLDVIRPADPEETAAAFSLAYARADGPTLLSLTRQNLPEGVAEQDPQGLIGGYVLSDAGGLQPGEEPDVILIATGSEVQLAVAAQTLLADNDILARVVSMPCLEWFEAQPYEYRDAVLPPTVSARVAVEAGVAQCWHQLVGDTGEIVSIEHYGESADHKTLFREYGF",
    "response#3": "LYLITPDTAEAPGVLAEQVAQAISGGARLVQYRQKGRDRQLRRAQAAAVLGVCRVAGVPFIVNDDVELAADIGADGVHLGRDDGDPITARRRLGDDAIIGVSCYDDMALARAAERAGASYVAFGSFFPSATKPHAVRASVERLSAARDSLRIPAVAIGGITPQNGGLLIAAGARMLAVVTGVFAQPGATAHAEWMLATRKTSQQVLTNMVQVLPELIGGSADLTPSNLTRWEGAVDFQPPITQLGNYAGRYIRYGVREHGMGAIMNGISAFGANYKPYGGTFLNFVSYAAGAVRLAALSGNPVIWVATHDSIGLGEDGPTHQPIETLAHLRAIPNMHVWRPADGNETSAAYYSAIKSGRTPSVVALSRQNLPRLSAAQRALYLITPDTAEAPGVLAEQVAQAISGGARLVQYRQKGRDRQLRRAQAAAVLGVCRVAGVPFIVNDDVELAADIGADGVHLGRDDGDPITARRRLGDDAIIGVSCYDDMALARAAERAGASYVAFGSFFPSATKPHAVRASVERLSAARDSLRIPAVAIGGITPQNGGLLIAAGARMLAVVTGVFAQPGATAHAEWMADHLRPALATRKTSQQVLTNMVQVLPELIGGSADLTPSNLTRWEGAVDFQPPITQLGNYAGRYIRYGVREHGMGAIMNGISAFGANYKPYGGTFLNFVSYAAGAVRLAALSGNPVIWVATHDSIGLGEDGPTHQPIETLAHLRAIPNMHVWRPADGNETSAAYYSAIKSGRTPSVVALSRQNL"
  },
  {
    "reference": "MVRYCVRSLSERSHEVYGQQLHGQEQGHHDQEEQGLSPEQVEVYERTQGHSHYRRRHCSRRRLHRIHRQQHRSCKRRRRHSCRHRRKHRRGCRTRRRTCRRH",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be able to modulate chromosome condensation, spermatid development in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "NSSLKGMSDFKKFEKSRELKNYIDIIRADYRKNLKSKVMLDRQIAVATYLIDVFALRAGGEKSEDEADTVGCCSLRYEHISLKPPNTVVFDFLGKDSIRYYQEVEVDKQVFKNLSIFKRPPKEPGHQLFDRLDPSILNKHLQNYMPGLTAKVFRTYNASKTMQDQLDLIPNEGSVTEKMVRYNAANRAVAILCNHQRTVGRSHAQSMQKATEKIEEYQWQKIRYKKR",
    "response#2": "EYPLTAPGPSWKKFQGSFCEFVKTLVYQCQYSLLYDGFPMDNLISLLTGLSDSQVRAFRHTSTLAAMKLMTSLVSVALQLSLHKDNNQRQYEAERNKGPGHRAPERLESC",
    "response#3": "LFRGRGAHPKTGKLKRRVQPEDVVLNLDKDAPIPEPPAGHKWGEIRHDNTVQWLCMWRENISNSFKYVRLAANSSLKGMSDFKKFEKSRELKNYIDIIRADYRKNLKSKVMLDRQIAVATYLIDVFALRAGGEKSEDEADTVGCCSLRYEHISLKPPNTVVFDFLGKDSIRYYQEVEVDKQVFKNLSIFKRPPKEPGHQLFDRLDPSILNKHLQNYMPGLTAKVFRTYNASKTMQDQLDLIPNEGSVTEKMVRYNAANRAVAILCNHQRTVGRSHAQSMQKATEKIEEYQWQKIRYKKAILQLDESELKANPKFFKEINDLTKEEQMSIHKRVLEREREKYQKKFARENEKRKFEGEELLPESQLESWLADVEEMGKEYVEELETGLVTVKPTLQSVEKLKAQIDKLEQRIRTTSIQMKDKEDNSTVALSTSKISYIDPRLSVAFCKKYL"
  },
  {
    "reference": "MEVNILGVIAVALFILIPTSFLLILYVKTSAENKDN",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The protein should be optimized for stability and expression in chloroplast thylakoid membrane, photosystem II where it will participate in photosynthesis, light reaction.3. The protein must exhibit the following characteristics: It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation; This subunit is found at the monomer-monomer interface.; One of the components of the core complex of photosystem II (PSII); PSII is a light-driven water:plastoquinone oxidoreductase that uses light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation. The designed protein sequence is ",
    "response#1": "SMSPQTETKASVGFKAGVKEYKLT",
    "response#2": "SCTF",
    "response#3": "GFSSII"
  },
  {
    "reference": "MSKIIGIDLGTTNSAVAVMEGSQPKIITNPDGGRTTPSVVAFKNGEIQVGDVAKRQAIVNPETISSIKRHMGESNYRVKANGKEYRPEEISAMILQYIKKYAEDYLGETVNDAVITVPAYFNDAQRQATKDAGKIAGLNVQRIINEPTAAALAFGLDKLDKDQKILVYDLGGGTFDVSILELGDGVFEVLSTNGDTHLGGDDFDQKVIDWLVEDFKKENGVDLSTDSLALQRLKDEAEKAKKTLSSANEAQILLPFIHQNLNIDKTLTRAQFNQLTADLVERAKGPVQNAMKDAGLSFSDINEVILNGGSTRIPAVQDSVKELTGKEPNHSINPDEAVALGAAIQGAVITGDVKDVVLLDVTPLSLGIETMGGVMTKLIDRNTTIPTSKSQVFSTAANNQPAVDIHVLQGERPMAADNKTLGNFQLTDIPAAPRGVPQIEVSFDIDRNGIVSVSAKDKGTGKSQKITIQNPGNLSEDEINKMVKDAESNEEADKKKKEEVDLHNQVDQLIFSSEKTLSDVGDKLGDSDKKPVQDALDELKKAKDSNNIETLKEKKDALEKAAQALAVKLYQQAGPQSGAAGQTGKAGQAGPNSDGKTGDDGKTVDGDFKEVNPDDKK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must have high specificity towards ATP-dependent protein folding chaperone, ATP binding, unfolded protein binding.2. The designed protein's functionality will benefit from Polar residues, Basic and acidic residues compositional bias in its structure.3. The protein must exhibit the following characteristics: Acts as a chaperone.. The designed protein sequence is ",
    "response#1": "SCGPATC",
    "response#2": "M",
    "response#3": "LSSG"
  },
  {
    "reference": "MEPVDPRLEPWKHPGSQPRTACTNCYCKKCCFHCQVCFIRKALGISYGRKKRRQRRRAPQDSETHQVSPPKQPASQPRGDPTGPKESKKKVERETETHPVN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that This binding increases Tat's affinity for a hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA) and allows Tat/P-TEFb complex to bind cooperatively to TAR RNA; To ensure maximal activation of the LTR, Tat mediates nuclear translocation of NF-kappa-B by interacting with host RELA; This also contributes to the increase in proviral transcription rate, especially when the provirus integrates in transcriptionally silent region of the host genome; Other factors such as HTATSF1/Tat-SF1, SUPT5H/SPT5, and HTATIP2 are also important for Tat's function; Besides its effect on RNA Pol II processivity, Tat induces chromatin remodeling of proviral genes by recruiting the histone acetyltransferases (HATs) CREBBP, EP300 and PCAF to the chromatin; Tat can reactivate a latently infected cell by penetrating in it and transactivating its LTR promoter2. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "MEPVDPNLEPWNHPGSQPTTPCSKCYCKKCCYHCPVCFLNKGLGISYGRKKRKQRRGTPQGSKDHQNPVPKQPLPMTRGNPTGPEESKKKVASKTETDTCA",
    "response#2": "MDPVDPNLEPWNHPGSQPTTPCSKCYCKKCCWHCQLCFLKKGLGISHGRKKRKHRRGTPQSHKDHQNPIPKQPLPTTRGNPTGPEESKKEVESKAETDPRD",
    "response#3": "MEPVDPNLEPWNHPGSQPNTPCTKCYCKVCSYHCQVCFLNKGLGISYGRKKRRQRRRAPPSSEDHQNPISKQPLPXTXGDQTGPEESKKKVESKTKTNPFD"
  },
  {
    "reference": "MALFRRFFYKKPPDRLLEISERVYVFDCCFSSDVMGEDEYKVYLGGIVAQLQDHFPEASFMVFNFREGEQRSQISDVLSQYDMTVMDYPRQYESCPLLPLEMIHHFLRSSESWLSLEGQQNVLLMHCERGGWPVLAFMLSGLLLYRKQYHGEQKTLEMVHKQAPKELLHLLSPLNPQPSQLRYLQYISRRNLGSDWPPSDTPLLLDCLILRDLPHFEGKKGCRPILRVYGQDPKARTNRSSILLFSTLKTKKHTRLYQQEECILVKLDIQCRVQGDVVLECIHLHDDLVSEEMVFRIMFHTAFVRANILMLQRDEMDILWDVKDQFPKEFKAEVLFSGADAVVPPITTSTLSDDENDFDMTSPEEFFEVEEIFSDVIDGPDHKRDSDSFVVVDTASDDSEGKEVWKGDVEPNAFLDCASDDSNHKHDMHAETSTDPVKDITVDDVQYRSDGKADSNIDSVKDIGIDDGDEQRKRRTVEAKENDSSTVQTQSKGDEESNDLESMSQKTNTSLNKPISEKPQATLRKQVGANAKPAAAGDSLKPKSKQQETQGPNVRMAKPNAVSRWIPSNKGSYKDSMHVAYPPTRINSAPASITTSLKDGKRATSPDGVIPKDAKTKYLRASVSSPDMRSRAPICSSPDSSPKETPSSLPPASPHQAPPPLPSLTSEAKTVLHSSQAVASPPPPPPPPPLPTYSHYQTSQLPPPPPPPPPFSSERPNSGTVLPPPPPPPPPFSSERPNSGTVLPPPPPPPLPFSSERPNSGTVLPPPP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be optimized for phosphoprotein phosphatase activity. The designed protein sequence is ",
    "response#1": "MVWEQKSRAVLMLNKLMEKKQIKCHLYWPNEMGADKALKLPHVKLTVELVRLETYQNFVRRWFKLTDLETQQSREVMQFHYTTWPDFGIPSSPNAFLKFLQQVRDSGCLSRDVGPAVVHCSAGIGRSGTFCLVDCCLVLIDKYGECNVSKVLCELRSYRMGLIQTADQLDFSYQAIIT",
    "response#2": "MVWEQKSRAVLMLNKLMEKKQIKCHLYWPNEMGADKALKLPHVKLTVELVRLETYQNFVRRWFKLTDLETQQSREVMQFHYTTWPDFGIPSSPNAFLKFLQQVRDSGCLSRDVGPAVVHCSAGIGRSGTFCLVDCCLVLIDKYGECNVSKVLCELRSYRMGLIQTADQLDFSYQAIIR",
    "response#3": "MVWEQKSRAVLMLNKLMEKKQIKCHLYWPNEMGADKALKLPHVKLTVELVRLETYQNFVRRWFKLTDLETQQSREVMQFHYTTWPDFGIPSSPNAFLKFLQQVRDSGCLSRDVGPAVVHCSAGIGRSGTFCLVDCCLVLIDKYGECNVSKVLCELRSYRMGLIQTADQLDFSYQAIIEGIQ"
  },
  {
    "reference": "MPNTHDTKNNVSPSEYAKFDPSTIHQRLNTSLSRPQLNSDGSIRHFLGVEGLNKAQLQAIIAKALFFEPSTRTRTTFEVAEKRLGANVLNLDIASSSAKKGESLRDTLWNLQAMTADIFVVRHSASGAAHFMATEVTPDIAIINGGDGWHAHPTQGMLDMLTIHREAPRPFEELSVAIIGDVKHSRVARSDISALQTLGVKDIRVIAPRTLLPKGIERFGVQVYEDMNSCVRDCDVIMGLRIQNERIGSPLLASSSEYYKQYGITPERVALAKPDALIMHPGPMNRGVEIASSVADGPQSVILKQVSNGVAIRMAVLALTMEGQRAHQANRG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be optimized for aspartate carbamoyltransferase activity, amino acid binding activity in order to improve amino acid metabolic process, 'de novo' pyrimidine nucleobase biosynthetic process, 'de novo' UMP biosynthetic process.2. The designed protein should interact with enzymes and substrates within the UMP biosynthesis via de novo pathway, (S)-dihydroorotate from bicarbonate: step 2/3, Pyrimidine metabolism pathway to enhance or regulate its activity.3. The protein should be designed to have high stability and activity towards carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-   aspartate + phosphate. The designed protein sequence is ",
    "response#1": "LLGIEELGRAEIEAILSRAKDFQPLQSQSLKKLDTLRGKMIVNLFFEASTRTRTSFEIAAKRLGADAISITASGSSVSKGESLVDTLNTLGAMRPDAIVMRHSASGAPHFLARYLPTPIINAGDGTHEHPTQALLDARTIAEHVGAFQRRMDGLKVAIIGDIAHSRVARSNVHLLSKFGAKIVLCGPASLLPVELAQLAPGVVLTTDIRDAIKDADVIMMLRVQLERQHEASFPASEYFRFYGLQLAHLDLAKPEAIVMHPGPINRGRELSSEVADFQRSVILNQVENGIAVRMAVLEGLKVAIAGDLDHSRVAKSNMQILKRLGAELYFAGPEEWRSQEFADYGKFVTIDEVIEEVDVMMFLRVQHERHDYESIFSKENYHRLHGLTQERYDRMKDTAILMHPAPVNRDVEIADHLVEAPKSRIVEQMTNGVFVRMAIIEAVLA",
    "response#2": "MKSVVTLDGLSTNQILDLLHKAEYIDSHRKEIAHTCDGRVLATLFYEPSTRTRLSFETAMLRLGGKVIGFAGAQLASVTKGESIADTLKTVSNYVDVVAIRHPKEGAALVASRAASVPVINAGDGGHMHPTQTLADLATLLAHLEHQLRPETEGLKVRIIGDLAHSRVARSNAEILNKLGATVTFSGPKDWQPVDFDSFGEHVDLDVGWSEQDVVMFLRVQHERITHTENQNFSAKKYHEVYGLTESRYENLKENSIIMHPAPVNRDVEIADDLVEAPKSRIFDQMTNGVYARMAILEALLAGE",
    "response#3": "LLGIEELGRAEIEAILSRAKDFQPLQSQSLKKLDTLRGKMIVNLFFEASTRTRTSFEIAAKRLGADAISITASGSSVSKGESLVDTLNTLGAMRPDAIVMRHSASGAPHFLARYLPTPIINAGDGTHEHPTQALLDARTIERIGADGINIGLLGDLRYGRTTHSLTYALSLYDAVIHTVAPAGLNLPSGLVHELRELGTEVIEHEQIEDVIKELDVLYVTRIQRERFPDTASYFNVASSYRITPELLRGTKEHMVVLHPLPRVDEIDPRVDKMKNARYFEQSHNGVPVRMAMLAGLVHGKREGLKVAIAGDLDHSRVAKSNMQILKRLGAELYFAGPEEWRSQEFADYGKFVTIDEVIEEVDVMMFLRVQHERHDYESIFSKENYHRLHGLTQERYDRMKDTAILMHPAPVNRDVEIADHLVEAPKSRIVEQMTNGVFVRMAIIEAVRA"
  },
  {
    "reference": "MYGIKIYNTLTRKIEEFKPVSPGFVKMYVCGPTVYDYNHIGHGRVYVVYDALKRYLALRGYHVLHVMNITDIDDKIINRAHQEKRDWREIAETYTRDYLESLGKLNVKVDLHPRVTEHIKEIIEFIQILIDKGYAYVAPSGSVYFEVDKYPEYGELSGRTNKELWSQEKEFISEKKKPYDFALWKAWKPGEPHWDAPWGKGRPGWHIECSVMSSRYLGRQFDIHGGGTDLIFPHHENERAQSEAAFGVKPWVKYWVHTGMVMMGSEKMSKSLGNIIPLREAFKEWGPETLRLWYLTSHYRRPLVFTEESIRQAQKYYERLVSVTNTIKKLSREAVSLHRMNDEDLKILEKLLEIRSRFHEALSNDFNTPQALAVISEFMTLVFKEIQYNPKYMLVLTAYKLLREFNTVLGVLDKYLVETPEELETLLDNVINIVVDIRRELRERKLYDLADRIRSELGKHGIILMDRGKETTWMRRK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should contain at least one 'KMSKS' region motif to achieve its intended function.2. The designed protein must be able to perform ATP binding under specific conditions.3. The designed protein should have a binding site that is specific to the target Zn(2+) ligand. The designed protein sequence is ",
    "response#1": "QKSNILILGPTGSGKTLLAQTLARMLKVPFAIADATNLTEAGYVGEDVENIILSLLQAADYDVERAQKGIVYIDEIDKIARKAESPSLTRDVSGEGVQQALLKIIEGTVASIPPKGGRKHPQQEFLKVDTSNILFICGGAFAGLDGVIQQRIGKKTLGFGADVKKKLEKKAGELLLQVTPEDLLKYGYIPEFIGRLH",
    "response#2": "QKSNILILGPTGSGKTLLAQTLARMLKVPFAIADATNLTEAGYVGEDVENIILSLLQAADYDVERAQKGIVYIDEIDKIARKAESPSLTRDVSGEGVQQALLKIIEGTVASIPPKGGRKHPQQEFLKVDTSNILFICGGAFAGLDGVIQQRIGKKTLGFGADVKKKLEKKAGELLLQVTPEDLLKYGYIPEFIGRLM",
    "response#3": "QKSNILILGPTGSGKTLLAQTLARMLKVPFAIADATNLTEAGYVGEDVENIILSLLQAADYDVERAQKGIVYIDEIDKIARKAESPSLTRDVSGEGVQQALLKIIEGTVASIPPKGGRKHPQQEFLKVDTSNILFICGGAFAGLDGVIQQRIGKKTLGFGADVKKKLEKKAGELLLQVTPEDLLKYGYIPEFIGRLI"
  },
  {
    "reference": "MRIVTSGEMYRIEEAAEKRGVSRRILMENAGKNIARNLANLFPDYKKKNICILCGKGNNGGDGLVAARYLKKMGAHPSVSILGRIHDISPLARENLSRLKKLKVRIHEIVKAEQIAEEIKNSSILIDAIFGTGFKGEIKGLYADVIDLLSFYTEGRRPSVVAVDLPSGMDADTGKISSPQIRADWTFTLGLPKKGLFLHPGIEYAGNILVLDIGIPQEIIGKGWLNLLSKEEISGFFPPRNTGAHKGSFGHTFILAGSPGFTGAAALAGTAALWSGAGLVTVGIPESLNTIMSVKLTEAMTKPFPETRAQTLGLKSFKGILEFSKKVDVLAIGPGLSRVGETRKLILKVIENINLPMVIDADALFALRGHTDILKRRRSPAVLTPHPGEMVYLTGKSLDWIQSHRIEYTRDFAVEFKVITILKGARTVIASPSGEVWVNPTGNPGMATGGSGDVLTGIIASLTGQGLNLLNASKAGVFLHGLAGDIAREEKGEYSLVASDIITKLPQAFKNVVL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration2. The designed protein must possess ADP-dependent NAD(P)H-hydrate dehydratase activity to support nicotinamide nucleotide metabolic process under certain conditions.3. The protein should be able to bind (6S)-NADPHX, AMP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "LLIVGGSAEYSGAPALSALAALRTGVDMAVVAAPKAAADAIRGFSPNLIVRSLSGEHLARDDLPRLYELAAQSDGIVVGPGLGLEAETREAVIEFVKMARGPILVDADAIKALSAKPASLKGKRSVVTPHAGEFAILTGRAMADPPNIEERMRQVKDASKNLGTTILLKAHEDIISDGERLKLNLTGNPGMTVGGTGDVLSGIAGAFLAWGAEPFRAACAAAFVSGKAGDLAKSEKGYHIVATDVVEKT",
    "response#2": "TYPIREIMSHKYTYGHVAVIGGSKGKTGAPVMAAQAALRSGAGLVTAIVPSSLNDVFENKLTEVMTIPVEDDGKGFLGIDSLEELLSVVEKGKFSSVVLGPGLGNSPETYELVREFVKECKLPMVIDADGINGLSESPEILKLKEEPVILTPHIGEFSRISGLSKEEILKDLTKVASDFSVHFGVTLVLKSGRTVISTPSGKTYVNIIGNPGMATAGTGDVLAGVVGALVGMGIESEDSAKFGVYLHSLAGDIVSKELSQESLVATDLINYLPRAVE",
    "response#3": "FLTAESIALLLRRRDRQAHKGHFGHCLIVAGSSGKTGAAALSANSAVRAGSGLVTLAVPESLNPILEVKTTEAMTVPLADSGGGYLTDSAFPAIAGLLPDKDALAIGPGLGQHPATVTLVRALLKAADLPLVIDADGLNAVAQNANCLLDKKSTHVVLTPHPGEMARLMGATIADVEADRIATARKFAAAFGVHLILKGSRTIIAAPDGSTAINGSGNPGMASGGMGDVLTGIIVSLLGQGYNCHDACRLGVFIHGYAADLVAQDKGEIGMCAGDVQEKLPYV"
  },
  {
    "reference": "MAAVVENGHHGNNGFCVKQNDPLNWVAAAEELKGSHLDEVKRMVEEFRKTVVKLGGETLTISQVAAIAARDNEVAVQLAESSRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGNGTESTHTLPHSATRAAMLVRINTLLQGYSGIRFEILETITKFLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGESLNAEQAFKLAGANSGLFFELQPKEGLALVNGTAVGSGLASIALYEANILSLLAEVMSAVFAEVMNGKPEFTDHLTHKLKHHPGQIEAAAIMEHILDGSSYVKAAQKMHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRTATKMIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNSRLAIASIGKLMFAQFSELVNDFYNNGLPSNLSGGRNPSLDYGFKGSEIAMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTVEALDILKLMSSTYLVALCQAIDLRHLEENLRLSVKNTISQVAKRTLTTGVNGELHPSRFCELDLLRVVDREYVFAYVDDPCSATYPLMQKLRQVLVEHALKNGENEKNASTSIFQKIEAFEAELKAVLPKEVESARVALEDGKPAIANRITECRSYPLYKFIREELGTNFLTGEKVMSPGEECDRVFTAMSKGLIVDPLLKCLEGWNGAPLPIC",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that This is a key enzyme of plant metabolism catalyzing the first reaction in the biosynthesis from L-phenylalanine of a wide variety of natural products based on the phenylpropane skeleton.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm that is critical for L-phenylalanine catabolic process.3. The protein should be designed to catalyze the following reaction L-phenylalanine = (E)-cinnamate + NH4(+).4. A protein that can modulate the overall flux of metabolites through the trans-cinnamate biosynthesis, Phenylpropanoid metabolism pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "TSGSTGTPQPHHHSWAALTQEAEALANALGSHPRVIAWLPVHHLYGFVFGVALPRALGSTVIESHAAPTALFREPAPDDLIATVPARWRYLFDSNHRFPGGTGISSTAALETACRNGLLQAGLDALLEVYGATEAGGIGLRRG",
    "response#2": "TSGSTGTPQPHHHSWAALTQEAEALANALGSHPRVIAWLPVHHLYGFVFGVALPRALGSTVIESHAAPTALFREPAPDDLIATVPARWRYLFDSNHRFPGGTGISSTAALETACRNGLLQAGLDALLEVYGATEAGGIGLRR",
    "response#3": "TSGSTGTPQPHHHSWAALTQEAEALANALGSHPRVIAWLPVHHLYGFVFGVALPRALGSTVIESHAAPTALFREPAPDDLIATVPARWRYLFDSNHRFPGGTGISSTAALETACRNGLLQAGLDALLEVYGATEAGGIGLRR"
  },
  {
    "reference": "MTTIRLKENEPVEVALRRFRREIERTGLIKELRARTAYEKPTTERKRKKAAAVSRTRKRLRSQMLPKKLY",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Prioritize Basic residues compositional bias within the protein sequence to achieve desired characteristics.2. The protein should be optimized for stability and solubility when expressed in ribonucleoprotein complex, ribosome where it will be involved in translation. The designed protein sequence is ",
    "response#1": "GTQVTFGEWGIQAMSPAYVTNRQIEAARIAMTRHIKRGGKVWINIYPDRPLTKKPAETRMGSGKGSPEWWIANVKPGRVMFEISGVSEDVAREALRLAIHKLPMKARII",
    "response#2": "MSLKIRLARGGRK",
    "response#3": "MF"
  },
  {
    "reference": "METLTELGDELTLGDIDEMLQFVSNQVGEFPDLFEEQLCQSYQGNNAMDTTLPKAYNQAAQQPYTTSAPQPQLLPVKAPPQATPQRTAPLLQPRPVVQTSPQPQLQQQTVMLTPNFSTAPQTRIIQQPLIYQNAATTSFQVLQPPVQSLMTTQQMQPVTIQQQVQTVQAQRVLTQAANGTIQTLTPATVQTVTPHVQQVPVLVQPQIIKTESLVLTAVKADGSPVMTAVQNPAITTLAGTLQTTALQVPTLMGSNGTILTTMPVMMGQEKMPIKQVPGSLKLAEVPKEGERRTTHNIIEKRYRSSINDKIMELKDLVMGTDAKMHKSGVLKKAIDYIKYLQQVNQKLRQENMALKLANQKNKYLKGIDLSSLVDTSIGMKIDEFNQNALMMSPPASDSGSPAVFSPYSVDSEPGSPLLDDEKVKDEPDSPTGLGMMDRSRMLLCTMTFLCLSFNPLTSLLHPESGQYSERAVQHGTGRTMLGVEMSGFYGSWFDWLIPTIILWLVNGVIVLSVFMKLLIHGEPVTRLHSRSSVKFWRHRKQADLDLAKGDFGAAALNLQTCLCVLGRSLPATRLDLACSLSWNIIRCSLQKISLVRWLLKHSPGYCKKAEFQDEATTSARDAALVYHKLHQLHLTGKLPSNWNCSGLNLALCAVNLAECAGNKISPTLLAEIHLTTAIQMKTSFPSRFRFLTAYFLGYAQNASSEETLPDPMRWLAHPLGKYFFINSNWALKSAAKDSLYTSTRNPANPVTQIHRAFCESLLEKAMYT",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should contain Cytoplasmic, Lumenal topological domains.2. A protein that contributes to regulation of transcription by RNA polymerase II, cholesterol metabolic process, SREBP signaling pathway, regulation of Notch signaling pathway, positive regulation of cholesterol biosynthetic process by functioning in ER to Golgi transport vesicle membrane, endoplasmic reticulum membrane, nucleus with protein dimerization activity, DNA binding.3. The protein must exhibit the following characteristics: [Processed sterol regulatory element-binding protein 2]: Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis; Regulates transcription of genes related to cholesterol synthesis pathway.; Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').4. The protein contains novel bHLH domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "QDCVWYGVCNTNDFSHSQNCPYNGTAKEMATDGLELLKKRCGFLLENSENKFCCDKNQVELLNKNVELAGNILDRCPSCMENLVRHICQFTCSPKQAEFMHVVATQKNKKGDEYISSVDLHISTEYINKTYKSCSQVSVPQTGQLAFDLMCGAYSASRCNPTKWFNFMGDATNPYVPFQITYIQHEPKSNSNNFTPLNVTTVPCNQAVSSKLPACSCSDCDLSCPQGPPEPPRPEPFKIVGLV",
    "response#2": "QDCVWYGVCNTNDFSHSQNCPYNGTAKEMATDGLELLKKRCGFLLENSENKFCCDKNQVELLNKNVELAGNILDRCPSCMENLVRHICQFTCSPKQAEFMHVVATQKNKKGDEYISSVDLHISTEYINKTYKSCSQVSVPQTGQLAFDLMCGAYSASRCNPTKWFNFMGDATNPYVPFQITYIQHEPKSNSNNFTPLNVTTVPCNQAVSSKLPACSCSDCDLSCPQGPPEPPRPEPFKIVGLQDCVWYGVCNTNDFSHSQNCPYNGTAKEMATDGLELLKKRCGFLLENSENKFCCDKNQVELLNKNVELAGNILDRCPSCMENLVRHICQFTCSPKQAEFMHVVATQKNKKGDEYISSVDLHISTEYINKTYKSCSQVSVPQTGQLAFDLMCGAYSASRCNPTKWFNFMGDATNPYVPFQITYIQHEPKSNSNNFTPLNVTTVPCNQAVSSKLPACSCSDCDLSCPQGPPEPPRPEPFKIVGLQGMGDSEAQDCVWYGVCNTNDFSHSQNCPYNGTAKEMATDGLELLKKRCGFLLENSENKFCCDKNQVELLNKNVELAGNILDRCPSCMENLVRHICQFTCSPKQAEFMHVVATQKNKKGDEYISSVDLHISTEYINKTYKSCSQVSVPQTGQLAFDLMCGAYSASRCNPTKWFNFMGDATNPYVPFQITYIQHEPKSNSNNFTPLNVTTVPCNQAVSSKLPACSCSDCDLSCPQGPPEPPRPEPFKIVGL",
    "response#3": "QDCVWYGVCNTNDFSHSQNCPYNGTAKEMATDGLELLKKRCGFLLENSENKFCCDKNQVELLNKNVELAGNILDRCPSCMENLVRHICQFTCSPKQAEFMHVVATQKNKKGDEYISSVDLHISTEYINKTYKSCSQVSVPQTGQLAFDLMCGAYSASRCNPTKWFNFMGDATNPYVPFQITYIQHEPKSNSNNFTPLNVTTVPCNQAVSSKLPACSCSDCDLSCPQGPPEPPRPEPFKIVGLQ"
  },
  {
    "reference": "MSLKGKKILLCVSGGIAVFKAAALTSKLYQTGAEVKVLMTKSATEFVTPLTFQTLSRNDVYKDTFEEKDPTAVAHIDVADWADLVLIAPATANIVGKLANGISDDMMSTTLLATEAPVWVAPAMNVHMYDHPAVKKNMDILRSFGCHFLEPGEGLLACGYVGKGRMAEPEDILATLETYFKEDTNDLAGKKVVVTAGPTREPVDPVRYFTNYSSGKMGFALAEQAANRGADVTLVAGPVSLPTPQGVERIDVVTAEEMFESVLQAAETADIVIKAAAVADYRPATIASEKVKKSEGEMVIEMERTKDILWTLGQKKKNQILVGFAAESERLDEFAQKKLVKKNLDLICANNIKAEGAGFDKDTNVMTLLHRDGERVELPLQSKHEAANRILDEVNRLDVTRS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The Proton donor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "GEVYLVGAGPGDPDLLTFRALRLMQRADVVLYDRLVAPAIVDLVRRDAERIFVGKEKSNHVVPQPEINELLLRLAQQGKRVLRLKGGDPFIFGRGGEEIEDLVRHNIRFQVIPGITAAAGCASYAGIPLTHRDHAQSCLFVTGHLKDGSVDLNWNALCQPRQTVVVYMGLTGLEVICREMIRHGRERSTPAALIQQGTTLNQQVITATLESLPDAVAAAQVEAPTLLIVGSVVTLAP",
    "response#2": "GEVYLVGAGPGDPDLLTFRALRLMQRADVVLYDRLVAPAIVDLVRRDAERIFVGKEKSNHVVPQPEINELLLRLAQQGKRVLRLKGGDPFIFGRGGEEIEDLVRHNIRFQVIPGITAAAGCASYAGIPLTHRDHAQSCLFVTGHLKDGSVDLNWNALCQPRQTVVVYMGLTGLEVICREMIRHGRERSTPAALIQQGTTLNQQVITATLESLPDAVAAAQVEAPTLLIVGSVVTLHG",
    "response#3": "GEVYLVGAGPGDPDLLTFRALRLMQRADVVLYDRLVAPAIVDLVRRDAERIFVGKEKSNHVVPQPEINELLLRLAQQGKRVLRLKGGDPFIFGRGGEEIEDLVRHNIRFQVIPGITAAAGCASYAGIPLTHRDHAQSCLFVTGHLKDGSVDLNWNALCQPRQTVVVYMGLTGLEVICREMIRHGRERSTPAALIQQGTTLNQQVITATLESLPDAVAAAQVEAPTLLIVGSVVTLHP"
  },
  {
    "reference": "MSRIGKAPIEIPAGVEVTVEGNLVRVKGPKGELSREIHPAMSITIENGALRVSRPSDEREHKALHGLTRTLIANMVEGVTKGYSKTLELVGTGYRAAKTGNQLTLSVGYSHPVVFNPPPGIEFQVPAPNQVIVAGIDKQLVGQVAADIRATRPPEPYLGKGIKYAGEHIRRKVGKAGK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.; This protein binds to the 23S rRNA, and is important in its secondary structure2. A protein that can perform rRNA binding, structural constituent of ribosome in ribonucleoprotein complex, ribosome to support translation. The designed protein sequence is ",
    "response#1": "MMLRNITGQEVVRCFAKRR",
    "response#2": "MSPVGIMVTLRSDRMYAFFDRLINLALPRIRDFRGVNPKSFDGRGNYSLGLREQLIFPEISYDSIDQMRGMDVSIITTATTDEEGRALLKVMGMPS",
    "response#3": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTV"
  },
  {
    "reference": "MLAAAPEFGRARIIVIGDVMLDRYWSGHAARISPEAPVPVVRVKSIEDRVGGAGNVALNIAKLGGKVTLLGVVGDDAEGDAIKRLLEAEGVVCDFVVEQDIRSICKLRVMAQHQQLIRIDFEETSLKFDSDALLARLVKHLPENNTVVFSDYGKGTLADVSAYIIEAKQAGLKTLVDPKGVDYRRYAQADLITPNLSELQTVVGVAENEENLIEKGRALLRRHQIPTLLLTRGEAGMTLIEDNQVYSLPAQAKDVFDVTGAGDTVIAVMALAVALDMALYDAMYLANLAGGIVVGKLGTSTVSMQELTRAMHGDRDSQYGIVSEDELAQILVGAKAHEERIIMTNGCFDLLHAGHVTYLQQAKALGDRLVVAVNSDASVRQLKGETRPINGLQERMTVLAALACVDWVVSFEEETPERLYCRLLPDVIVKGGDYSSEQVAGGDCVIKAGGEVKILQFVDGQSTTAMIKKARRLE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Ideal protein should have a binding affinity for key enzymes in the Nucleotide-sugar biosynthesis pathway to enhance the overall efficiency of the pathway.2. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.3. The active site of the protein must be engineered to enable effective catalysis of ATP + D-glycero-beta-D-manno-heptose 7-phosphate = ADP + D-   glycero-beta-D-manno-heptose 1,7-bisphosphate + H(+).4. The designed protein should contain one or more Cytidyltransferase-like, Carbohydrate kinase PfkB domains that are essential for its function.5. A protein with heptose-1-phosphate adenylyltransferase activity, ATP binding, heptose 7-phosphate kinase activity, phosphotransferase activity, alcohol group as acceptor is needed to enhance lipopolysaccharide core region biosynthetic process, ADP-L-glycero-beta-D-manno-heptose biosynthetic process in a specific context.6. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7- phosphate at the C-1 position to selectively form D-glycero-beta-D- manno-heptose-1,7-bisphosphate.. The designed protein sequence is ",
    "response#1": "MIQDYSDAGVLIIGDVMLDRYWYGPTGRISPEAPVPVVKVEQNEERPGGAANVAMNIASLGGKARIVGLTGKDEPAQVLTDKLSSLNVMCDFVALDDYPTITKLRILSRGQQLIRLDFEDKFENTDPALVLTKMDQALPNVKAVILSDYAKGALEHVQQYIQKARAAGVPVFIDPKGADFERYRGATILTPNMSEFEQVVGKVTSDEDLVEKGRALIEQFDFEALLVTRSEHGMTLLRRDQEPFHLPTQAKEVYDVTGAGDTVISVLASSVAAGKPMDEACALANAAAGVVVGKLGTSTVSTIELAEALTPGSGIARVLVVGDVMLDRYWHGNTSRISPEAPVPVVHVGDEEHRAGGAGNVALNISALGGNVTLIGFAGKDEAGESLEGILHNAGVNCCFFQQQGFSTITKLRVISHHQQLIRLDFEDSFAGADLRQLEQLFVEQLDSHDVVILSDYGKGALQDVQALIKLAKKADKTVLVDPKGSDFRKYAGATLITPNFSEFQGVVGTCPHEQIAEKGLELITGCDLASLLVTRGEHGMMLLSKDKEPLNLPTHAREVFDVTGAGDTVISVLGASLAAGSELPDAVKLSNVAAGVVVGKLGTAEAAAQQEFAAMLIEDARAHGEKIVFTNGCFDILHAGHVTYLEQAAAQGDRLIVAVNDDASVSRLKGPGRPINSVDRRMAVLAGLGAVDWVISFAEGTPEKLLEQVRPDVLVKGGDYGIDQVVGADIVKAYGGEVRVLGLVENSSTTAIVEKIRSGEQS",
    "response#2": "MTIPAFAQAPVLVVGDVMLDRYWYGSTTRISPEAPVPVVKVKRTEDRPGGAANVALNIAALGAPVQLIGVTGADEAADALIEQLHSVGVQTHFQRIADQPTIVKLRAMSRQQQLLRMDFEESFNTDADALLATVESILDSVKVMVLSDYGKGSLRNHQALLQAAKQRGVAVLADPKGTDFSIYRGATLLTPNLAEFEAVVGHCADELQLMQRGLAMLQELELEALLITRGEQGMTLLRHDVPALHLPARAREVFDVTGAGDTVISTMAATLAAGESLPQAMTLANLAAGIVVGKLGTATISAPELRRALIAAERNRGKRIVFTNGCFDLLHFGHVKYLQKARSLGDLLVLGLNSDASVRRLKGAKRPLIDQDERAHLLAALDCIDYVVIFDEDTPLELITALKPHILAKGGDYSLDGVVGREIVESYGGRVELITFVDGKSTTNIIERVLAGHTRNRMTNGCFDLLHEGHVAYLEQARRLGDRLIVAVNDDDSVRRLKGEGRPVNTLTRRMAVLAGLASVDWVVPFSEDTPERLICAVRPDVLVKGGDIEDARAHGEKIVFTNGCFDILHAGHVTYLEQAAAQGDRLIVAVNDDASVSRLKGPGRPINSVDRRMAVLAGLGAVDWVISFAEGTPEKLLEQVRPDVLVKGGDYGIDQVVGADIVKAYGGEVRVLGLVENSSTTAIVEKIG",
    "response#3": "VLIIGDVMLDRYWYGPTGRISPEAPVPVVKVEQNEERPGGAANVAMNIASLGGKARIVGLTGKDEPAQVLTDKLSSLNVMCDFVALDDYPTITKLRILSRGQQLIRLDFEDKFENTDPALVLTKMDQALPNVKAVILSDYAKGALEHVQQYIQKARAAGVPVFIDPKGADFERYRGATILTPNMSEFEQVVGKVTSDEDLVEKGRALIEQFDFEALLVTRSEHGMTLLRRDQEPFHLPTQAKEVYDVTGAGDTVISVLASSVAAGKPMDEACALANAAAGVVVGKLGTSTVSTIELATLESEIPKDLVLTEEQLKAAVKRARARGEKVVMTNGCFDILHAGHVSYLNNAAELGDRLIVAVNTDESVKKLKGPGRPVNSTERRMAVLAGLGAVDWVVPFSEETPQRLISEVLPTLLVKGGDYKPEEVAGGKEVIAAGGEVRILNFEDGCSTTEIIEAIRT"
  },
  {
    "reference": "MTTISKNKTSNSKGKTTTLGTILKPLNSKYGKVLPGWGTAGIMLIFMTLFAIFLTIILEIYNSSVILDIK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be designed to localize specifically to photosystem II in order to perform its role in photosynthesis, protein stabilization more effectively.2. The protein must exhibit the following characteristics: It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation.. The designed protein sequence is ",
    "response#1": "MF",
    "response#2": "MGXRXFXG",
    "response#3": "MDL"
  },
  {
    "reference": "MTSLPKTSSPRVCVIGAGPCGIACVKNLLEQGIDNIDVFEKNNRIGGNWVYDEKGTHSSVYETTHLISSRWLSWFEDFPMPPGTSEYPSHEQVLHYFEVYAARFGMARFVRFETRVEKVEPLDEKRWQVTYSDAEGLHTGVYDYVMVANGHHWDPSMPEYPGTFSGEMLHAHAYKKAEPFRGKRVLVVGGGNSACDIAVEVARVARKSCISMRRGQHIIPKFLFGKPSDTVLARIKWLPYRLRQIVAGSVLRALQGHYGKYRLQKPRERLFASHPTINSELLYAIRHGKVFPCRGIERFEGSTVHFTDGEHEDFDVVIFATGYKTTFPFFAPELIDYSNARQLPLYLKMMHPTFETLYFIGLFQPQGCIWPLADYQAGIAARIMAGTRSRPKRLEQKIQGEIKAHKARFHDSPRHALEVDYHAFRRQLRKALKA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The ideal protein would be one that plays a critical role in lipid metabolic process through flavin adenine dinucleotide binding, NADP binding within membrane.2. The protein should be designed to have high stability and activity towards H(+) + hexan-3-one + NADPH + O2 = ethyl butanoate + H2O +   NADP(+). The designed protein sequence is ",
    "response#1": "MSIILGVDPGSRITGFGVIRQQGRQLTYVGSGCIRTSSADLPLRLKQ",
    "response#2": "MN",
    "response#3": "MNMIKLERS"
  },
  {
    "reference": "MQPLYKSCYPLDNRCYEEYNLTEEILMEHASMGMANYIKQNFKEQSSVLIVCGVGNNGADGLVLARHLQNSYEVKVYLPFGVRSVMAEVQLERVQTLDMEFVDEVEEADVIVDALFGAGLSRELDEKTRKLIIKLEKLNGFKIACDVPTGIDVDGNPLPMALFCDMTITMGVLKECLYTDMAKEYVGEVICVDLGLSHAKYTQGFKSDIFLLEEHDMRLPTRRRANTHKGSFGHLAVIAGEKEGAGIMSGMAALRFGAGLVSLVGEVETVLPLEIMASHELPHNTTAIAFGMGFDAFEDEIVDDVLESDIPLILDAGVFSNEIILEFLEQKDREIVLTPHPKEFVALWNMTIDSPLNIAILQANRFEKVREFCELFPQVTLLLKGANLIVAKDKELFINPYGSSKLSKGGSGDVLAGLIGSLLAQGYDGLEATIQASLAFTSAAKAYAGSSYAMLPTDVIDEVGKLESKIK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have a high affinity for (6S)-NADPHX, K(+), AMP.2. The designed protein have YjeF N-terminal domains with specific sequence motifs that are crucial for its activity.3. The protein should be designed to have high stability and activity towards (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.4. The designed protein should have ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding, metal ion binding, NADPHX epimerase activity, NADHX epimerase activity to facilitate nicotinamide nucleotide metabolic process.5. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.6. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. The designed protein sequence is ",
    "response#1": "MMITDINCECLGLSRLCLMESAGKSLAEEVGKIAVYTFSKPVKIAIFTGSGGNGGDGFVAARYLLNRGYDVDIYMLTDKDNIHSDNAKTNFEIITNMEPRLSHLTVHYLDTIEDVNNCDIAQSESFSEFIIVDAILGTGIKGELRPKVKRAVEVINKSNGLTVSVDVPSGMDPLTGEVSDVAVEPHYTISFHRVKTGIHLAGEDKVGGIVTCDIGIPIEAEESLLTPAPLTRRKVCSHKGDYGHVLVVGGDYGYAGAVHLAAKAALNSGAGLVSVATRKEHALQTHLFSPELMGHALEQLADISELVNRATVLVLGPGLAQRKWAKSIWPTLIKIDLLRVIDADALNLLAKDPAYSTNWILTPHPGEAARLLHCSTADILQDRYQAVRSIQQKYGGVCVLKGAGTLVCGGEDVYVNRTGNPGMASGGVGDVLAGLIGSLLAQQYPLIEAAEQGVYRHGLAADKVAANKGERGLCASDVVDYLIQVVAESDKYRRGVLGVAAGSHLYTGAAVLAVGGALRAGAGMVRYAGAEEPVAQVRAHWPEAVITVLDRPSIDEVGRVQAWVLGPGLGTGERAHDLAASVLASDVPVLIDADGLTIVAKDRELLRRTAPVLLTPHAGELARLTGAERADIEAARLEHVRAAAAELGVTVLLKGSTTLIAGPSGQARVNVTGSSWLATGGTGDVLSGVAGALLAQGLSCYDAAACGAYVHGLAGVQ",
    "response#2": "MMITDINCECLGLSRLCLMESAGKSLAEEVGKIAVYTFSKPVKIAIFTGSGGNGGDGFVAARYLLNRGYDVDIYMLTDKDNIHSDNAKTNFEIITNMEPRLSHLTVHYLDTIEDVNNCDIAQSESFSEFIIVDAILGTGIKGELRPKVKRAVEVINKSNGLTVSVDVPSGMDPLTGEVSDVAVEPHYTISFHRVKTGIHLAGEDKVGGIVTCDIGIPIEAEEPSDIGAMLPARSVDAHKRSTGSVLVLAGSDGMSGAALLTSRGAARMGAGYVTLVSTSYVDHAKKSLIPEAVSRIVADHSELGPEVLAEFDDDFDRADAVALGPGLGTGPRQQALVETALEAIDVPLVLDADGLNVLAEDAAALRKRTSPTVLTPHPGEMATLLKLSSKDVQADRVGVARRAASDLGCVVVLKGAGTIVAGPEGPAVVNPTGGPELATAGTGDVLTGVVGALLAEGLDPVSAAIAGVFVHGLAGGVAGVSAGGRGTVAWDVAEALPAAVGELYDAHKRSTGSVLVLAGSDGMSGAALLTSRGAARMGAGYVTLVSTSYVDHAKKSLIPEAVSRIVADHSELGPEVLAEFDDDFDRADAVALGPGLGTGPRQQALVETALEAIDVPLVLDADGLNVLAEDAAALRKRTSPTVLTPHPGEMATLLKLSSKDVQADRVGVARRAASDLGCVVVLKGAGTIVAGPEGPAVVNPTGGPELATAGTGDVLTGVVGALLAEGLDPVSAAIAGVFVHGE",
    "response#3": "MMITDINCECLGLSRLCLMESAGKSLAEEVGKIAVYTFSKPVKIAIFTGSGGNGGDGFVAARYLLNRGYDVDIYMLTDKDNIHSDNAKTNFEIITNMEPRLSHLTVHYLDTIEDVNNCDIAQSESFSEFIIVDAILGTGIKGELRPKVKRAVEVINKSNGLTVSVDVPSGMDPLTGEVSDVAVEPHYTISFHRVKTGIHLAGEDKVGGIVTCDIGIPIEAELALGGSPGFTGAITLTARAALRAGAGLVTAAVPRGCQPVVAAGAPEIMTWGLPETAGGSLGRSALDPLLERLENFSVLAIGPGLGRDPETVELVKSLLPRASIPLVVDADALNALATDTGILSQPHGPMVLTPHPGEMGRLIGCPAAKIQEDRLNIARRYAQEWRCVLLLKGARTVVACPDGRAYINPTGNPGMATGGSGDVLTGIIAGLIAQGMSLDKAAAIGAYLHGAAGDLAAEELGQEALIAGDILHEDVARLLPRPGAESDKYRRGVLGVAAGSHLYTGAAVLAVGGALRAGAGMVRYAGAEEPVAQVRAHWPEAVITVLDRPSIDEVGRVQAWVLGPGLGTGERAHDLAASVLASDVPVLIDADGLTIVAKDRELLRRTAPVLLTPHAGELARLTGAERADIEAARLEHVRAAAAELGVTVLLKGSTTLIAGPSGQARVNVTGSSWLATGGTGDVLSGVAGALLAQGLSCYDAAACGAYVHGLAGRLAADGAPLAAADVATAVPAL"
  },
  {
    "reference": "MNYRVPSLKATALAMAALTQALTTWDAAYEKALADLASLTQSEKVGVVSGITWEGGPCVGNTYAPESIAYPSLCLQDGPLGIRFANPVTAFPAGINAGATWDRELLRARGAAMGEEAKGLGVHVQLAPVAGALGKIPSAGRNWEGFTSDPYLSGIAMAETIHGMQGSGVQACAKHYILNEQEHSRETISSNVDDRTMHEVYLWPFYDAVKANVASVMCSYNKINGTWACENEGILDTLLKQELGFRGYVMSDWNAQHSTVASANTGLDMTMPGSDFSQPPGSIYWNENLAEAVANGSVPQARVDDMVTRILAAWYLLEQDQGYPAVAFDSRNGGKASVDVTADHADIARTVARDSIVLLKNSNNTLPLRNPSSIAVVGSDAIVNPDGPNACTDRGCNVGTLAQGWGSGTAEFPYLVAPLDAIQERSSGNGTKVVTSTTDDATAGADAAASADIAIVFISSDSGEGYITVEGHQGDRNNLDPWHGGNDLVKAVAAVNKKTIVVVHSTGPVVLETILAQPNVVAVVWAGIPGQESGNALADVLYGDVSPSGKLPYTIGKSEADYGTTWVANGADDDFPEGLFIDYRHFDKNEIEPRYEFGFGLSYTRFNFSNLAINIDATSGPTSGAVDVGGAADLYDSVGTISATVTNVGGVSGAEVAQLYIGFPSSAPETPPKQLRGFQKLPLAGGADGVAEFELTRRDISYWDVGQQKWVVPEGSFQVYVGASSRDIRLDGSFTV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The active site active site of the designed protein must be optimized for substrate binding and specificity.2. The designed protein should be expressed specifically in the extracellular region3. The protein must exhibit the following characteristics: Beta-glucosidases are one of a number of cellulolytic enzymes involved in the degradation of cellulosic biomass. The designed protein sequence is ",
    "response#1": "IVLMKNDDNILPLKKDKPILVIGPNAKIAAYCGGGSASLDPYYTVTPFEGVSAKSTGAVTFSQGVYSHKQLPELGPLMKSADGKKGFSFRVYKEPVSAPSRELVDELHLVSSSGFLMDYVHPKIDSLTFYVDMEGYFTPEEDGVYDFGVTVVGTGKLLIDGETVVDNTKNQRPGSAFFGTATVEEQGSKELKAGQTYKVVLEFGTAPTSDLDTRGVVVFGPGGFRFGASRRVSQEELIAKAADAAAQAEQVVIFAGLTSEWETEGHDRDHMDLPPGSDEMIQRVLAANPNTAVVIQSGTPVTMPWAAQTKALVQAWFGGNECGNGIADVLYGDVNPAGKLPLSFPVRLQDNPSYLNFRSERGRVLYGEDVYVGYRYYEKVNLAPLFPFGHGLSL",
    "response#2": "VVLLKKNEANVLPLVKDSKTRYALIGDHWKNPAVAGDDSSEVTPYYVSTPYSAFVEAVGEDSFICAMGCYSHKFAPLLYSTITQPGSDAHGMLLEFFNKDPNGSSDAELLYTTTTEKTDLKFADSLPPDTVPEYTSSGSAPSRGSRWGCAGWIREAVEIARQVDIPVILTGLSADYEYEGIDRKSLGLPGRVDELIERVTEANPKTIIITEAGTATTMPWADKTPTVIHSWFGRQETGHGIVDILFGDVNPSGRLPLTFPRNLEVPPVYF",
    "response#3": "VVLLKKNEANVLPLVKDSKTRYALIGDHWKNPAVAGDDSSEVTPYYVSTPYSAFVEAVGEDSFICAMGCYSHKFAPLLYSTITQPGSDAHGMLLEFFNKDPNGSSDAELLYTTTTEKTDLKFADSLPPDTVPEYTSSGSAPSRGSRWGCAGWIREAVEIARQVDIPVILTGLSADYEYEGIDRKSLGLPGRVDELIERVTEANPKTIIITEAGTATTMPWADKTPTVIHSWFGRQETGHGIVDILFGDVNPSGRLPLTFPRNLEVPPVYR"
  },
  {
    "reference": "MTSVLDVTNRDRLIESESLAARTLQERLRLVEEVLVDVLAAESGQELVDLLRRLGALSSPEGHVLHAPEGELLKVIESLELNEAIRAARAFNLYFQIINIVEQHYEQQYNRERAAQEGLRRRSVMSEPISGVSGEGFPLPHTAANATDVRSGPSERLEHSLYEAIPATQQYGSFAWLFPRLQMLNVPPRHIQKLLDQLDIKLVFTAHPTEIVRQTIRDKQRRVARLLEQLDVLEGASPHLTDWNAQTLRAQLMEEIRLWWRTDELHQFKPEVLDEVEYTLHYFKEVIFAVIPKLYRRLEQSLHETFPALQPPRHRFCRFGSWVGGDRDGNPYVKPEVTWQTACYQRNLVLEEYIKSVERLINLLSLSLHWCDVLPDLLDSLEQDQRQLPSIYEQYAVRYRQEPYRLKLAYVLKRLQNTRDRNRALQTYCIRRNEAEELNNGQFYRHGEEFLAELLLIQRNLKETGLACRELDDLICQVEVFGFNLAALDIRQESTCHAEALNEITAYLGILPCPYTELSEAERTRWLLSELSTRRPLIPGELPFSDRTNEIIETFRMVRQLQQEFGTDLCNTYIISMSHEVSDLLEVLLFAKEAGLFDPATGASTLQAIPLFETVEDLKHAPAVLTQLFSLPFCRSYLGSNSTPFLQEVMLGYSDSNKDSGFLSSNWEIYKAQQQLQKIAESFGFQLRIFHGRGGSVGRGGGPAYAAILAQPAQTIKGRIKITEQGEVLASKYSLPELALFNLETVATAVIQASLLRSSIDEIEPWHE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design must be focused on enhancing the efficiency of carbon fixation. The designed protein sequence is ",
    "response#1": "YPFWAQQNYESPREATGKIVCANCHLAQMPTIAEVPQSVGADSVFKAVVKIPYKNDLKEIGADGSEVPLQVGAVVMLPDGFKLAPQERWTEEIKEETEGVYFTNYSEEQDNIIIVGPLPGDTNKEIVFPVLSPDPSTNKEYHYGKYSLHIGGNRG",
    "response#2": "NKNNNSYIPMIQYSNIHCIDWSSLISVDKLDLGSSNIFLIATFLNNKCEYNQVLLKSSTTIVQEVYASVLESILKLPIPEMRLLEYSNDEYIQMSRSLLTISSKTNKSLYDYIKLELLKSFFLIMEYAPNGKSFKDLDYKEYFSGYGGEKKLNQLGQVIAFDIFCNNSDRLPLIWDNVSSHFSNILFYDIPNRNGWYISLMNSNVTCINNSSFTIGYRKYITRVKSILYALFKRPNIESNQIKKMRESISKSYKINLSSSCGISIQRGIIQGC",
    "response#3": "GISGLRAKLGAPRRALTGSALKPQGLAPEALAGLAHRLALGGVDLIKDDHGIADQAFSPFAARVPAVARAMREACAVRGAAMLYAPHVSGSLDDMRRQLDIVRREGLSVVMLMPMIVGLANFHLIAKEAEGLIVLAHPSLAGAQRIAPDLLLGKLFRLLGADATIFPHYGGRFAYTPETCRALADAARRDWHDLKPCLPVPAGGIAIDRIKELLAFYGTDVMLLIGGSC"
  },
  {
    "reference": "MVIVASSNMLRPDRIWLCGKYQTARTLYVVGNKSKRRNPSLEDWARSDSDRKMKLQQQEYNRKLKELKNLTANVSKMISKKSEKEVERSQIQSTEPSPETSTQLTQKNDSQRLDFLAKKSLFIPVVDIPASVSDRIGLAFKYLVSKSSQNWSMVLDQLEKNGGFKDIPSKDIRKFVYQIPKPHIPPIISRLKKMHHDAGVPVSPKLVNVFIDSLLLSPSIPSSVMSQIESYCDSIRSTSKKGRLPRETYELLIRAYGKNSNLEMVNSILTEMKQVGLQPSKNTFENILATSVYKSKDHKQAVEVFDTMKFLSDKTKPAERAYRDIIVSYVNNDDIEKALDLYNEMVENKVEVSQQIMVALARGCISRPELKVKAWEFIFEIYNQKWEPTIQTLEYVLYLAAKDGDVALCRALVNKLSETSSVTVRSFSFLLLGYSKSSLYNESPSIPPILAHENGIRFRRNILADSSYAPSSESSLPFLPVLDLVTEKEILAESSAMWAYANTIRPDLINIESANTYLNIGAKFGSLKEFLDRYNSATLLDRQGVPDTRVIEDENLAEVSVMDYTSTASVRSPLLDRNHTLKVPRDDMTYLIALKAASKARNYEFSQEVWSERGLYRKSDRIRLSQEQKRTNLIFNLQSLWSQL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in mRNA processing, RNA splicing, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "NLTQQYNAPHSLAGALRIAEHYDCLSSFPPLDPIIDFGGSWWHHYSRKDTRIHSCCPVLGVRDAARHEERLCRMRKLLQECDDREDLPDFCIDRAESCSVQADWAICIHGGYDMGYTGLCEAMHSHGVRILRGTIMFDGAMLFDNEGVLPLLKCRWMKSGKGKSEVIKFDFMNESTLSYIHSWTNLGSFN",
    "response#2": "DLPKDKSDRTHQRINLNNSGTDRTNDLYLHIVQTFGCIETTATENATKLLMLGDVEVEISASSVSIEWTQKSMISQTIADSIVIMIIGLCASDKNVLSESELKERNHNVWKIQELQNLFREQFGDSFSIDEGIGKKENVKNGSVTIGKSKATIDFSTMKLIDCNSNPLKGRVESILSIGQKLTTPR",
    "response#3": "DLPKDKSDRTHQRINLNNSGTDRTNDLYLHIVQTFGCIETTATENATKLLMLGDVEVEISASSVSIEWTQKSMISQTIADSIVIMIIGLCASDKNVLSESELKERNHNVWKIQELQNLFREQFGDSFSIDEGIGKKENVKNGSVTIGKSKATIDFSTMKLIDCNSNPLKGRVESILSIGQKLTTPG"
  },
  {
    "reference": "MMDIFLAILPAIFWGSIVLFNVKLGGGPYSQTLGTTFGALIFSIVVYIFMKPVLTPTVIGVGVVSGLFWALGQANQLKSIDLMGVSRTMPISTGLQLVATTLFGVIVFHEWSTTISVVLGILALVCIIIGVILTSLQSEEEKNAEQAANFKRGIVILLISTVGYLVYVVVIRLFNVDGWSALLPQAVGMVLGGILLTFKHHPFNKYAIRNIIPGLIWAAGNMFLFISQPRVGVATSFSLSQMGIIISTLGGILILGEKKTKRQLTGIVVGIVFIIAAGIMLGIAKS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein localizes to the plasma membrane.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The protein must exhibit the following characteristics: Involved in the uptake of glucose.. The designed protein sequence is ",
    "response#1": "GRTANGKVRLYVIGQGALSAAVAANSKHDIAEAERWNLPIILIVLLAVFGSLAAAAVPLALGVCTVVVTMGLVDLVSMHTIMSVFVTSTVSMFGIALAVDYSLFILMRFREELRSGRQPQEAVDAAMATSGLAVVLSGMTVIASLTGIYLINTAALKSMATGAILAVAIAMLASITLTPAALATFGRAAVKRSVLMHWSQRSECTQSLFWTRWVGWVMHRR",
    "response#2": "GRTANGKVRLYVIGQGALSAAVAANSKHDIAEAERWNLPIILIVLLAVFGSLAAAAVPLALGVCTVVVTMGLVDLVSMHTIMSVFVTSTVSMFGIALAVDYSLFILMRFREELRSGRQPQEAVDAAMATSGLAVVLSGMTVIASLTGIYLINTAALKSMATGAILAVAIAMLASITLTPAALATFGRAAVKRSVLMHWSQRSECTQSLFWTRWVGWVMHRR",
    "response#3": "GRTANGKVRLYVIGQGALSAAVAANSKHDIAEAERWNLPIILIVLLAVFGSLAAAAVPLALGVCTVVVTMGLVDLVSMHTIMSVFVTSTVSMFGIALAVDYSLFILMRFREELRSGRQPQEAVDAAMATSGLAVVLSGMTVIASLTGIYLINTAALKSMATGAILAVAIAMLASITLTPAALATFGRAAVKRSVLMHWSQRSECTQSLFWTRWVGWVMHRR"
  },
  {
    "reference": "MTSNSHVKIGIIGGSGLDDPDILEGRTERYVVTPYGKPSDALILGKIKNVDCVLLARHGRQHTIMPTNVNYQANIWALKEEGCTHLLVTTACGSLREDIQPGDIVLIDQFIDRTTKRVQTFYDGQPTSPPGVCHIPMAEPFCSKTREVLLEVAQGLGVKCHTRGTMVTIEGPRFSSRAESLMFRQWGADVINMTTVPEVVLAKEAGLCYASIAMATDYDCWKEHEEAVCVDNVLKTMKENANKASSILLTAIPQICQMDWDSTINAHKSMSQSSVMLPKH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that is capable of catalyzing reactions within the S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'- thioadenosine (phosphorylase route): step 1/1, Amino-acid biosynthesis pathway with high efficiency and specificity.2. The protein must exhibit the following characteristics: Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis; Catalyzes the reversible phosphorylation of S-methyl-5'- thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate; Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine; Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates..3. The protein should be designed to have high stability and activity towards phosphate + S-methyl-5'-thioadenosine = adenine + S-methyl-5-   thio-alpha-D-ribose 1-phosphate.4. The designed protein should have S-methyl-5-thioadenosine phosphorylase activity within nucleus, cytosol to regulate purine ribonucleoside salvage. The designed protein sequence is ",
    "response#1": "MKIGIIGGSGVYGIFEPKETVKVHTPYGRPSAPVEIGEIEGVEVAFIPRHGKYHEFPPHEVPYRANIWALHELGVERVIAVNAVGSLKEEYKPGDIVIIDQFIDFTKKREYTFYNGPRVAHISMADPFCPELRRIFIETAKELNLPVHEKGTYICIEGPRFSTRAESRMFRQFADVIGMTLVPEVNLARELGMCYVNISTVTDYDVWAEKPVDAQEVLRVMKENEEKVQKLLKRAEIGDGNLNLVFKIRDTAGISRVIVKQALPYVRCVGESWPLTLDRARIEAETLLTHSQFCPQHTVKVLHHDAELAVMVQEDLSDHHIWRHELIQGNYYPQAAEQLGEYLAQTLFHTSDFYQSAQAKKAAVSRYTNPELCQITEDLFFTDPYIDHERNNFDPVLLPEVLSLRQDKALKLAVASLKHRFLSQAEALLHGDIHSGSIFVADGRLKTIDAEFGFYGPIGFDIGTALGNLLSAGREIGIVPQLGVIGGSGVYDIDGLEGARWQTVESPFGDVSDQILRGTLDGLEMAFLPRHGRGHVLAPSDVNYRANIDALKRAGVTEILSVSAVGSLAEDLPPGTFVIADQFIDRTFAREKSFFGKGLVAHVSMAHPVSAWLGDRVEEVLADLAIPHRRGGTYLCMEGPQFSTLAESNLYRQWGCHVIGMTNMPEAKLAREAEIAYCTVAMVTDFDCWHPDHDHVSVEAVVRVLLQNADKARSLVKILDKEGYGEYITHRG",
    "response#2": "MAKIGIIGGSGVYGVFEPKEVVKVHTPYGRPSAPIEIGEIEGVEVAFIPRHGKYHEFPPHQVPYRANIWALHELGVERVIAINAVGSLKEEYKPGDIVIIDQFIDFTKKREYTFYNGPKVAHVSMADPFCPELRKIFIETAKELNLPVHERGTYVCIEGPRFSTRAESRMFRQFADVIGMTLVPEVNLARELGMCYVNISTVTDYDVWAEKPVDAQEVLRVMKENEEKVQKLLKRALDELKF",
    "response#3": "MNKIGIIGGSGVYGVFEPKEVVKVHTPYGRPSAPIEIGEIEGVEVAFIPRHGKYHEFPPHQVPYRANIWALHELGVERVIAINAVGSLKEEYKPGDIVIIDQFIDFTKKREYTFYNGPKVAHVSMADPFCPELRKIFIETAKELNLPVHERGTYVCIEGPRFSTRAESRMFRQFADVIGMTLVPEVNLARELGMCYVNISTVTDYDVWAEKPVDAQEVLRVMKENEEKVQKLLKRALERFTKPVSNASQPRIAVIGGTGLRELPGFTQVASLSITTPWGSPSSPITILHHQCSHNNKTVAVAFLSRHGTHHQIAPHEVPARANIAALRSIGVRTIIAFSAVGSLQEEIKPRDFVIPDQVIDRTKGVRPWTFFEGGVVAHVPFGDPFDEGVAKVVRACGHSLEGEGVVLHDRGTLICMEGPQFSTRAESNLYRSWGGSVINMSCLPEAKLAREAEIAYQMICMSTDYDCWHESTADVTVEMVMGHMKANAQNARRFVTAVKTKKLFPEVTVPEEIAVLGGVGFNSHKDCESHPVTTPYGRITAYLTSIKGRSVVIIPRHAEEIHIPPHRVNYRGNIWAAHSLGAKRVISTNSVGSMRGHPVGSFVVLDDFIDFTRSRPSTFHDDKTVHVDVSEPYCPEIRASLRYSLEKRGISYTEGVYACTEGPRFETRAEIRMMSQFADVVGMTGVPEVVLAKELSLCYASLSIVTNQACGMTTQKLTADEVTEVVGKAQASIFKILSDA"
  },
  {
    "reference": "MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKLHQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSSAIEAVIGFLYLEKREERLEALLNKIITIVNER",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The ideal protein would be one that plays a critical role in rRNA processing through rRNA binding within cytoplasm.2. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. For general function, the protein need meet that Processes 30S rRNA precursor transcript even in absence of ribonuclease 3 (Rnc); Rnc processes 30S rRNA into smaller rRNA precursors.; Involved in correct processing of both the 5' and 3' ends of 23S rRNA precursor The designed protein sequence is ",
    "response#1": "LLFVIRVKGPTAVKIPAKAYKVLSLLRLVELNTGVFIKLTKDVYPLLKLVSPYIVIGQPSLASIRSLIQKRSRIMWQRPEDKEPKEIILNDNNIVEEKLGDEGVICIEDIIHEISTLGENFSKCTFFLLPFKLNREVSGFGAISRLNKLKMREQNKKTRQISNAATAPVIQVDIDSMISKLR",
    "response#2": "LLFVIRVKGPTAVKIPAKAYKVLSLLRLVELNTGVFIKLTKDVYPLLKLVSPYIVIGQPSLASIRSLIQKRSRIMWQRPEDKEPKEIILNDNNIVEEKLGDEGVICIEDIIHEISTLGENFSKCTFFLLPFKLNREVSGFGAISRLNKLKMREQNKKTRQISNAATAPVIQVDIDSMISKLR",
    "response#3": "LLFVIRVKGPTAVKIPAKAYKVLSLLRLVELNTGVFIKLTKDVYPLLKLVSPYIVIGQPSLASIRSLIQKRSRIMWQRPEDKEPKEIILNDNNIVEEKLGDEGVICIEDIIHEISTLGENFSKCTFFLLPFKLNREVSGFGAISRLNKLKMREQNKKTRQISNAATAPVIQVDIDSMISKLR"
  },
  {
    "reference": "MTDERGNFYYNTPPPLRYPSNPATAIFTSAQTYNAPGYVPPATVPTTVATRDNRMDYTSRSNSTNSVAIAPYNKSKEPTLDAGESIWYNKCVDFVQKIIRYYRCNDMSELSPLMILFINTIRDMCIDTNPISVNVVKRFESEETMIRHLIRLQKELGQSNAAESLSSDSNIFQPSFVLNSLPAYAQKFYNGGADMLGKDALAEAAKQLSLAVQYMVAEAVTCNIPIPLPFNQQLANNYMTLLLKHATLPPNIQSAVESRRFPHINMINDLINAVIDDLFAGGGDYYHYVLNEKNRARVMSLKENVAFLAPLSASANIFNYMAELATRAGKQPSMFQNATFLTSAANAVNSPAAHLTKSACQESLTELAFQNETLRRFIFQQINYNKDANAIIAAAAPNATRPNTKGRTA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein can be expressed in host cell nucleus, virion component and perform structural molecule activity without triggering undesirable cellular responses.2. For general function, the protein need meet that Major tegument protein that participates in the egress of nucleocapsids from the host nucleus; May be involved in host nucleus- cytoplasm transportation of nucleocapsids to form budded virion (BVs). The designed protein sequence is ",
    "response#1": "LGVPD",
    "response#2": "SCPLV",
    "response#3": "GPR"
  },
  {
    "reference": "MDTAVIILAAGKGTRMXSXRPKVLHEVGNFPLIXHCIXXAXSIDPKKILIVLGHGQSEIKKVLGNEVTSIDFAIQEEQLGTGHAVGVATNELADFDGNLIVLFGDTPFISAKTMTKIENECKQADLVVLGFHPQDPTGYGRLVTKNKELLRIVEHKDANDEELAITLCNSGVMAGSAVLIKKLLKELDRNNKNNEIYLTDCIELAKTEGLSSTYLTCDPTEALGINSPQQLAYAEKRFQQTARNVALNNGIHLVAPETVFFSLDTNLGQDTVIEPNVIFGPGVTIGTGCKVKAFSYLEGCKISKDCNIGPFARIRPGVDLSKGVKIGNFVEIKNAKVAEHSKINHLSYIGDAEIGQNTNIGAGTITCNYDGFNKHKTIIGEDVFIGSNTMLVAPVNIGNNGITGSGSVITKDVPAQDLAIARSEQRNKKGGAKKLKALLSGKKNSKEK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Mg(2+) is required for the proper folding and stability of the protein.2. The protein should be able to modulate regulation of cell shape, cell morphogenesis in a way that leads to a desirable outcome.3. A protein that is capable of catalyzing reactions within the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, Nucleotide-sugar biosynthesis pathway with high efficiency and specificity.4. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain..5. The designed protein have MobA-like NTP transferase domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "VILAAGKGTRMQSDLPKVLHKLAGAPLLAHAMQSGAALDPEHTVVVAGHGGDAVTAAAQDQHPEARVALQTEQLGTAHAVAQARTALEGFGGDVIVLYGDTPFISEDTLLTMLEARTRADVVVLGFLGMRAEDPTGYGRLLRDADGRVAAIVEQKDATDAERAINEVNTGILVAPTAALKRWLSSLQANNAQGEYYLTDIVAMAVADGVPVVTAQPADVWEVEGVNDKVQLAALERRFQRWQAERLMREGVTIVDPEHTYIGADVKIGQDTVLMPGSLLEGNTVIGEDCVIGPESRLVNARVASGARIEKSVVLDSFVDVGARVGPFAYVRPGSEIGPEARIGDFVEIKNTRVGTGTKISHLAYVGDADVGEGVNVGCGVITVNYDGERKHHTVIGNGSFVGSNVNLIAPVRVGAGAYVTAGSTITDDVPEDAFAIARSRQRNIDGWRRPQK",
    "response#2": "VILAAGKGTRMQSDLPKVLHKLAGAPLLAHAMQSGAALDPEHTVVVAGHGGDAVTAAAQDQHPEARVALQTEQLGTAHAVAQARTALEGFGGDVIVLYGDTPFISEDTLLTMLEARTRADVVVLGFGMRPADPGRYGRLVMAGGELVAIREEKDATPEERAVDLCNSGVFAFDVQALRAHLGAIRNDNAQGEFYLTDCVEIARADGLRVSAVEAPEAEVLGANDRVQLGELEIAHRARCAERLMARGVTIQDPANTYISADAEIGRDTVLMPGTVIEGRTVIGEDCLIGPGSQIRDSVIGDRTAIRQSVVMSSKIGEDTAVGPFAHIRPETDLGNSVKIGNFVEVKKSNLGEGSKVSHLSYIGDSDIGERVNIGCGTITVNYDGVNKFRTTVEDDAFVGCNSNLVAPVTVGKGAFVAAGSTVTKNVPENALAIARSRQRTLEGWAARYRAGKAADKAAK",
    "response#3": "MTTPKVLHPVAGKALVSHVIDTARSLSPQTLCLVYGHGGDTLRNTLDAPDLAWVLQEPQLGTGHAVQQALPHLKQEGTTLILYGDVPLIQSETLNRLVHAAHDALAILLGMRPDDPGRYGRLVMSGDTLDRIVEFKDASEEECAVTLCNSGVIAADCRELLDLVGAVGNANAQGEYYLTDIVGIARARGLTASVVECPEAETLGINTRAELARAEAAFQARARAAAMAGGVTIQDPANTYISADAEIGRDTVLMPGTVIEGRTVIGEDCLIGPGSQIRDSVIGDRTAIRQSVVMSSKIGEDTAVGPFAHIRPETDLGNSVKIGNFVEVKKSNLGEGSKVSHLSYIGDSDIGERVNIGCGTITVNYDGVNKFRTTVEDDAFVGCNSNLVAPVTVGKGAFVAAGSTVTKNVPENALAIARSRQVSKPGLATKLFEALRAAKAAKS"
  },
  {
    "reference": "MAVKNLEKGINVSGRVWKSEKDAFRATSKVIKNKKLTSWELKREQRQLDQQFKERMNALKNEKEEERQQRIKALRERREKKEEKERYERLAARMHAKKVERMRRREKRNKALKER",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be designed to localize specifically to nucleolus in order to perform its role in rRNA processing more effectively.2. The protein must exhibit the following characteristics: Involved in nucleolar integrity and required for processing of the pre-rRNA for the 60S ribosome subunit.. The designed protein sequence is ",
    "response#1": "GKELKVACSQSCSRLMEKLISLSDMRQIRRLFNKFIGHFLHLVQHRFASHCCETLFINAAPGVTEKISKSKGRKAEVDEEDEPEPELSLAEMFMKVVEELEGNWGYLLTERFASHTIRVLLLVLAGEPVDLSSTDSVVASRKKERLGIVNTEASEENAVAERRKVPEVFEATLKKVMNDMVSGLDNTYLRALATHPVGNPVLQVLVFLELSHFGKASAKDPNSIIRRLIPDDDFGENAESSTFVRGLLYDPVGSRLLETIIKSMPGKLFKSLYKNIIRDRIGSLARNTTAGYVVLRTLERLGKDDLQDAMELIIPEIPGLIERSRLIVPKVLIERCLVRGVDTKPLSDALESAYDKNPARRLEQMLKLEPAASEEDSEEKQKQTGNNQTAAAEKLHGSLLAQTMLTASGPVSGLIYSSLLAQSAESLLRLAKDPTASRVLQQALTTPTSTSQFRRQFTTRFSGHLVELALDSSGSHVVDALWPATKDLFFVKERMAQELLKEELSLRDSFVGRR",
    "response#2": "GKELKVACSQSCSRLMEKLISLSDMRQIRRLFNKFIGHFLHLVQHRFASHCCETLFINAAPGVTEKISKSKGRKAEVDEEDEPEPELSLAEMFMKVVEELEGNWGYLLTERFASHTIRVLLLVLAGEPVDLSSTDSVVASRKKERLGIVNTEASEENAVAERRKVPEVFEATLKKVMNDMVSGLDNTYLRALATHPVGNPVLQVLVFLELSHFGKASAKDPNSIIRRLIPDDDFGENAESSTFVRGLLYDPVGSRLLETIIKSMPGKLFKSLYKNIIRDRIGSLARNTTAGYVVLRTLERLGKDDLQDAMELIIPEIPGLIERSRLIVPKVLIERCLVRGVDTKPLSDALESAYDKNPARRLEQMLKLEPAASEEDSEEKQKQTGNNQTAAAEKLHGSLLAQTMLTASGPVSGLIYSSLLAQSAESLLRLAKDPTASRVLQQALTTPTSTSQFRRQFTTRFSGHLVELALDSSGSHVVDALWPATKDLFFVKERMAQELLKEELSLRDSFVGRC",
    "response#3": "GKELKVACSQSCSRLMEKLISLSDMRQIRRLFNKFIGHFLHLVQHRFASHCCETLFINAAPGVTEKISKSKGRKAEVDEEDEPEPELSLAEMFMKVVEELEGNWGYLLTERFASHTIRVLLLVLAGEPVDLSSTDSVVASRKKERLGIVNTEASEENAVAERRKVPEVFEATLKKVMNDMVSGLDNTYLRALATHPVGNPVLQVLVFLELSHFGKASAKDPNSIIRRLIPDDDFGENAESSTFVRGLLYDPVGSRLLETIIKSMPGKLFKSLYKNIIRDRIGSLARNTTAGYVVLRTLERLGKDDLQDAMELIIPEIPGLIERSRLIVPKVLIERCLVRGVDTKPLSDALESAYDKNPARRLEQMLKLEPAASEEDSEEKQKQTGNNQTAAAEKLHGSLLAQTMLTASGPVSGLIYSSLLAQSAESLLRLAKDPTASRVLQQALTTPTSTSQFRRQFTTRFSGHLVELALDSSGSHVVDALWPATKDLFFVKERMAQELLKEELSLRDSFVGRC"
  },
  {
    "reference": "MAERRVPFSLLRGPSWDPFRDWYPAHSRLFDQAFGLPRLPEEWSQWLSHSGWPGYVRALPAAAIEGPAYNRALSRQLSSGVSEIQQTADRWRVSLDVNHFAPEELTVKTKDGVVEITGKHEERQDEHGYISRCFTRKYTLPPGVDPTLVSSSLSPEGTLTVEAPLPKSATQSAEITIPVTFQARAQLGGPEAGKSEQPENK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be optimized for nucleus-specific folding and stability, while maintaining protein folding chaperone.2. The protein must exhibit the following characteristics: Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.. The designed protein sequence is ",
    "response#1": "SKPGFEKTVINKKAGRKPDENLSEEEREQRMKQFVKENEKLCQQYGMLRKYDDSKRFLQEHLHLVGEETANYLVIWSINLEMEEKHELMAHVAHQCICMQYILELAKQLDVDPRACVSSFFSKIQHCHPEYRAQFDSEIEGFKGRIQKRAQEKIQEAIAQAEEES",
    "response#2": "ALSRQLSSGVSEIRQTADRWRVSLDVNHFAPEELTVKTKDGVVEITGKHEERQDEHGYISRRLTPKYTLPPGVDPTLVSSSLSPEGTLTVEAPMPKPATQSAEITIPVN",
    "response#3": "ALSRQLSSGVSEIRQTADRWRVSLDVNHFAPEELTVKTKDGVVEITGKHEERQDEHGYISRRLTPKYTLPPGVDPTLVSSSLSPEGTLTVEAPMPKPATQSAEITIPVR"
  },
  {
    "reference": "MLIGLVRDSVMQCFCHTCRAPGILPAVSRRSAPIKKNNKIRSKQPRLSKKVKFITTEIRCQEKSRGGLSYEVILAEPAPNVAVPKRPVTPGKNVSVEEIEQKLKAAEERRISLEAKKMADISTKLAKVEEATRKKDEITNEFITQTKEQLESKMELHVEKREAIISDMKEKLKIHAQDIEKTRETLEQQKANEQKAIEEKLKIAQSLRDENIKKMLDRLKEHERRAELVRQNKAQAQDLDGQQSAIASSG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should emphasize Polar residues compositional bias.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm that is critical for germ cell migration. The designed protein sequence is ",
    "response#1": "HLTHSCLRSVLEQIAAYGQVVFRLQQFIDEVMGHSSESMLPGSGSVPKKSTEAPFRTYQAFMWALYKYFISSKEELAEIEKCIINNDATITLAIVVDKLAPRLAQLKVLHKVFSTGVAEVPPDTRNVVRASHLLNTLYKAILEYDDVGEASEQTVSLLFSLWVETVRPYLQTVDEWIVHGHLWDGAREFIIQRNKNVPVNHRDFWYATYTLYSVSEKTENEEKMSDNASASSGSDQGPSSRQHTMVSFLKPVLKQIIMAGKSMQLLKNLQCAESTTCQAGARDAERKC",
    "response#2": "LVEYLQAMRNFFLMEGGDTMYDFYTSIFDKIREKETWQNVSFLNVQLQEAVGQRYPEDSSRLSISFENVDTAKKKLPVHILDGLTLSYKVPWPVDIVISLECQKIYNQVFLLLLQIKWAKYSLDVLLFGELVSTAEKPRLQEGLVREQDTVAQFGPQKEPVRQQIHRMFLLRVKLMHFVNSLHNYIMTRILHSTGLEFQHQVEEAKDLDQLIKIHYRYLSTIHDRCLLREKVSFVKEAIMKVLNLALMFADGWQAGLGTWRMESIEKMESDFKNCHMFLVTILNKAVCRGSFPHLESLALSL",
    "response#3": "STCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGDVPPGPLPEAILALYNSTRDRVAGESAETEPEPEADYYAKEVTRVLMVEYGNKIYDKMKSSSHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLTRREEIEGFRLSAHCSCDSKDNTLQVDINGFSSGRRGDLATIHGMNRPFLLLC"
  },
  {
    "reference": "MTGNAVEAPAGRAGSSRRVPLVGLSRPQMMQALATAGVPEKFLRMRTGQLWRWIYGHGLTDFAPMSTISKSLRAEFAARFTLERPRIVSEQVSEDGTRKWLMRFAPASGETVGAEVETVFIPEENRGTLCISSQVGCTLNCTFCHTGTQRLARNLGAGEIVSQVLVARDHLGEWPAGPDRQITNVVLMGMGEPLYNFEAVKTAVDIIADGEGLSLSRRRITLSTAGVVPMIPRCGEEMGVMLAISLHASNDATRNRLVPLNRKYPIHELLEACRAYPGLSNARRITFEYVMIRDVNDSRADAKALVRLLAGIPAKINLIPFNPWPGSEYACSSWERIERFAEIINRAGYASPIRSPRGRDIMAACGQLKSASQKQRRQITR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design must be able to perform tRNA (adenine-C2-)-methyltransferase activity, rRNA (adenine-C2-)-methyltransferase activity, rRNA binding in order to contribute to rRNA base methylation.2. The protein must be engineered to have a cooperative binding mechanism with [4Fe-4S] cluster to ensure proper regulation.3. The protein with the reaction adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine, the S-methylcysteine intermediate, Proton acceptor must be optimized to ensure efficient catalysis.4. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. The designed protein sequence is ",
    "response#1": "PRRHLLDLSLAELRDWVVERGEKPFRAAQVWRWIYQGRAADFESMTDLSRSLRQHLAEDFVLFAGSIARHQTSPDGTEKLLIGLADGGEVECVLLRDGPRRSICVSSQVGCAMGCVFCASGLDGVDRNLTRGEILEQMLRLQHRLDHEERLSHIVMMGMGEPLANLERVLSALDVARSPEGLGISPRRITISTVGLPPAIDRLARHGVPYNLAVSLHAPEDELRNRLVPVNRKIGVEALMAAADRYFEASGRRLTFEYVLLGGLNDSPTQAAQLGHLLRGRGAMLNVIPYNPVAGLPYRTPTSTATSRFREVLERGGINVNFRVRKGGEIDAACGQLRRNRQATAELPEPDLYAYPQRNTICISSQAGCGMACPFCATGQGGLNRNLSTAEILEQVRAAAAALRDDFGGQRLSNVVFMGMGEPLANYPRVLAAVRRITERSGFGISARAVTVSTVGLAPAIRKLADERLGVTLALSLHAPDDELRDTLVPVNNRWKIAEALDAARYYAELTGRRISVEYALIRDVNDQPWRADLLGKRLHRVLGPLVHVNLIPLNPTPGSDWDASPKAVEREFVRRVRSKGVSCTVRGDKGSDIAAACGQLGRAHLLDLSFAEFQQWLVEHGQPKFRAKQVFGWVYEKRARDFDSMSDLPKTLREQLASEFVIYRSDEAAVQTSRDGTDKLLVRLADGGEVECVLLRDGNRRSICVSSQVGCAMGCVFCASGLDGVDRNLTRGEIVEQMLRLQARLEPEERLSHIVMMGMGEPLANLDRVLGALETAKSADGLGISPRRITISTVGLPPAIDRLANKGVPYNLAVSLHAPNEELRSQLVPVNEKIGIDAVLAAADRYFAASGRRLTFEYVLLGGVNDGAEHARELAQLLRHRTVLLNVIPYNPVEGLPYQTPSQRAIAEFRQILESAGVNVMFRQRKGDEIDAACGQLRRNR",
    "response#2": "TTQVGCKVGCTFCASTLGGLKRNLTPGEIVAQVVQAQKILDAKGERVSSIVIMGTGEPFENYDATMKFLRIMIHEKGLNIGQRHITVSTSGIVPNIYRFADENTQINLAISIHAPNDALRSKLMPVNRRFPFDDVMEALRYYIAKTGRRITFEYALIGGVNDQPEHDYPPMPGRAHLLDLSFAEFQQWLVEHGQPKFRAKQVFGWVYEKRARDFDSMSDLPKTLREQLASEFVIYRSDEAAVQTSRDGTDKLLVRLADGGEVECVLLRDGNRRSICVSSQVGCAMGCVFCASGLDGVDRNLTRGEIVEQMLRLQARLEPEERLSHIVMMGMGEPLANLDRVLGALETAKSADGLGISPRRITISTVGLPPAIDRLANKGVPYNLAVSLHAPNEELRSQLVPVNEKIGIDAVLAAADRYFAASGRRLTFEYVLLGGVNDGAEHARELAQLLRHRTVLLNVIPYNPVEGLPYQTPSQRAIAEFRQILESAGVNVMFRQRKGDEIDAACGQLRRNRLARLL",
    "response#3": "DGDRATLCVSSQVGCKMGCAFCQTGKQGFDGNLTAGDILNQIYALPEREQLTNIVMMGQGEPMDNVENVLAAIEVMTASWGYGWSPKRITVSTVGLRKGLRKFLNGCDCHLAVSLHSPIHEQRLNMMPAEGAMTIDEVVETLREYDFSHQRRLSFEYIVFKGLNDSRMHAKEIVKLLSGLDCRINLIRFHEIPGVDLPSSDEEGMLRLRDYLTAHGFFTTIPWHGRAHLLDLSFAEFQQWLVEHGQPKFRAKQVFGWVYEKRARDFDSMSDLPKTLREQLASEFVIYRSDEAAVQTSRDGTDKLLVRLADGGEVECVLLRDGNRRSICVSSQVGCAMGCVFCASGLDGVDRNLTRGEIVEQMLRLQARLEPEERLSHIVMMGMGEPLANLDRVLGALETAKSADGLGISPRRITISTVGLPPAIDRLANKGVPYNLAVSLHAPNEELRSQLVPVNEKIGIDAVLAAADRYFAASGRRLTFEYVLLGGVNDGAEHARELAQLLRHRTVLLNVIPYNPVEGLPYQTPSQRAIAEFRQILESAGVNVMFRQRKGDEIDAACGQLRRNRPG"
  },
  {
    "reference": "MVFNKFVQVGRVVYINYGADKGKLAVIVNIINQNRILIDGEHIVRQVIPIRRVHLTKFQIDNVELNQRTVLLKKKIAKFDLTKKYAETSFAKKQAIKTKRANLGDFDRFRVMVLKKKLARTSAKSK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that can perform structural constituent of ribosome, RNA binding in ribonucleoprotein complex, ribosome to support translation. The designed protein sequence is ",
    "response#1": "MPQPL",
    "response#2": "XGIMQVPKVEKIIINMGVGEAVANAKSLDTAIEDLQIIAGQKPVVTKAKKSIAGFKLG",
    "response#3": "MKTPPAADQLRKIVGKGSGEPNTKKAGNVTLDQIRAIAENKMSDLNANDITNAMRMIEGTARSMGVTL"
  },
  {
    "reference": "MAPSKKAGKKGAVGGFLSGASKPQKVQKADWSEGFTKKKAAGVPDMTLLSTITNEAINDNLKVRFQNQEIYTYIAHVLISVNPFRDLGIYTNDVLNSYRGKNRLEMSPHVFAIAESAYYRMTTEKENQCVIISGESGAGKTEAAKRIMQYIAAVSGGAEGGGIEGIKEMVLATNPLLESFGCAKTLRNDNSSRHGKYLEIMFNGMGQPVGAQITNYLLEKNRVVGQIDDERDFHIFYQFTKGASAKMKEAFGLQGPEAYAYISRSGCLDVKSINDVSDFQETLRAMQVIGLTSDEQDSIFRILATILWLGNIDFVEGDDGNAAISDSGVADFAAYLLEVDSAQLQKVLLMRIMETQRGGRRGSVYEVPQNVAQASSGRDALAKALYNNLFEWIVSRVNISMKPQTPSQYVIGVLDIYGFEIFQDNSFEQLSINYVNEKLQQIFIELTLKAEQEEYVREQIKWTPIKFFDNSVVCSLIEDRRPAGIFATLNDATATAHADPSAADNSFIQRSSMLASNPNFEARGNKFLIKHYAGDVLYTVAGMTDKNKDTLIKDILDLIEGSKDPFLHTLFPDKVDHTSKKRPPTAGDKIKLSANLLVENLMNCQPHYIRTIKPNQHRSPTEYDDKAILHQIKYLGLQENIRVRRAGFAYRAEFSKMIQRFYLLSPATSYAGDYIWTGDDRSGCERILTDAKIKKEEWQMGVTKAFIKNPETLFYLEGERDRYWHTMASRIQRAWRAYVRRKHEAATKIQRFWRNQREALVYERKRDY",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be designed to localize specifically to plasma membrane in order to perform its role in actin filament organization more effectively.2. The designed protein must have at least one IQ domain.3. For general function, the protein need meet that Required for proper actin cytoskeleton polarization The designed protein sequence is ",
    "response#1": "LMNDFWEMETISFPPDGSSITPAHHYNDFRFKVYAPIAFRYFRDLFGIAPDDFLMSMCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLWPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNSLMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGKQVY",
    "response#2": "MCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLWPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNSLMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGL",
    "response#3": "MCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLWPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNSLMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGC"
  },
  {
    "reference": "MEARAQARYIRVTPMKARRVVDLIRGMDATEAQAVLRFAPQAASVPVGKVLDSAIANAAHNYDHTDADSLFISEAYVDEGPTLKRFRPRAQGRAYRIRKRTSHITVVVSSKEGTR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be involved in ribosome assembly, translation by localizing to cytosolic large ribosomal subunit, large ribosomal subunit and exhibiting structural constituent of ribosome, rRNA binding.2. The protein must exhibit the following characteristics: The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.. The designed protein sequence is ",
    "response#1": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#2": "MPPIGCKVTLRGEAAEGFLETSLGIVEKRLNESQFDIFGNVSFGVEEHTDYPGMRYDPNIGIFGMDITVVVNRPGYRVSKRRIAKRKIPTSHKITKEDTIE",
    "response#3": "MPIGCKVTLRGEAAEGFLETSLGIVEKRLNESQFDIFGNVSFGVEEHTDYPGMRYDPNIGIFGMDITVVVNRPGYRVSKRRIAKRKIPTSHKITKEDTII"
  },
  {
    "reference": "MQRISGHSELPVFDVAASRRIEQASAAALPPHTLMRRAGLAVAQLAAALVPHARTIWIACGPGNNGGDGFEAAAQLKSRGFDACISFAGDEARLPPDALDALQRARQAQVPFLTERPAEHDLAIDALLGLGATRAPAEGMARWLERIYRCGAPVLAVDLPSGLDADTGVARFDFGHGGARHCLNLLTLKPGVFTAQGRDAAGEVWFDDLGCAPGQEVAAALLNTEPRRESRAHATHKGSYGDVAVLGGAQGMAGAALLAASAALHGGAGRVFVALLDKARPSLSTVQPELMFRNPAAMDWTQPTVVCGCGGGTAVQALLPRVLAETPRAVLDADALNAIAADGSLQSAVAARRDRGQQTVLTPHPLEAARLLGTNTAGVQCDRLKAARALAAQFQSVVVLKGSGSVIAAPDEAPFINLSGNALLATGGTGDVLAGMIGAELAAGHEALAAARRAVWHHGALADGWPAGRPLTAGALAGG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..2. The protein should be designed to catalyze the following reaction (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.3. The (6S)-NADPHX, AMP binding site should be stable and able to withstand changes in environmental conditions.4. The protein must bind to Mg(2+) in order to perform its enzymatic function.5. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.6. The protein must exhibit kinase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity. The designed protein sequence is ",
    "response#1": "MKACEKRSEELGFPASELMANAGKVLASHVISETRREDQPDTPEVVILAGKGNNGGDGLVAANFLNKAGISTKIVLCCGKPATPAAINAYDSLNKDIDVLFYDGDNSHPWAAAIDDASLVVDAIFGTGFTGEIERDLQPLFIKLNTYARRVIACDIPSGVDARSGQAGVLAVQADITVTMHAKKLGMLLSPAKYFCGYIRVEDIGIPAAATVLIVGGQPGMSGAVILAMQSALRAGAGKVSAACHPQVQAIVAAAQPEAMVHGIKETDSLEPLLREASAVALGPGLGQGSWSQEIFSQVMETDVLKVIDADGLNLLASNPVRSPKLLLTPHPGEAARLLQMTTDEIASDRFAAATKLREKYGAQVLLKGAGSLLVTDRGRCLIDRGSPAMASGGMGDLLTGLIAGLLAQRMAPAQALIAGSLWHAIAGEEAGKDGERGTLASDLLPELTLLV",
    "response#2": "MKACEKRSEELGFPASELMANAGKVLASHVISETRREDQPDTPEVVILAGKGNNGGDGLVAANFLNKAGISTKIVLCCGKPATPAAINAYDSLNKDIDVLFYDGDNSHPWAAAIDDASLVVDAIFGTGFTGEIERDLQPLFIKLNTYARRVIACDIPSGVDARSGQAGVLAVQADITVTMHAKKLGMLLSPAKYFCGYIRVEDIGIPAAATLAEIPRPIAAVPGPDSHKYTRGLVTIIAGDMPGASALAASAAMRSGAGVVRLQAREFVPGVPAAVIQMPGDPLDRLDDKRIGALLLGPGLRSGRELLDAALASGHPLVLDAGALAMLADGGPERIEGAILTPHDGEFQKLFGALEGSKVDRARASAAQSGAVIAYKGPDTVVAAPDGRAAIHGTAPPWLGTAGSGDVLAGIIAAERAAGKEPFEAACAGVWRHAEAARRAGPKMIADDLIGAGKVSLATSPEHVAASLVRCPEVMAHGVRGGAEASMLPSSDVVVVGPGLGQDAWGQAILQSALQSELPLVVDADALNLLASRWSEVRRDNWVLTPHPGEAARLLGCSVADVQADRPAAARALQRERGGVVILKGAGSLVAGPSGLVVCPYGNPGMASGGMGDVLSGMLGTLIAQNDSLELAAWLGVIVHAQAGDDAAKNAGERGLLASDLF",
    "response#3": "MRFLEKRVFKMGVTPEALMDSAGRGIADAIRRRYAVPGEAIACIGRGNNGGDALVALKYLAQAGWVISVHCAVESNHLYALPRKKWRELDVINNDRSSLPLGPRIVLDGLLGIGASGPLRGSIAEAAEWINRQRGCPNTFVIAMDIPTGINGNSGVPCKEAVIADLTLTVGICKRGLLMPAAVNHTGSIETVLLDELQIPKDGEVLIVGGQPGMSGAVILAMQSALRAGAGKVSAACHPQVQAIVAAAQPEAMVHGIKETDSLEPLLREASAVALGPGLGQGSWSQEIFSQVMETDVLKVIDADGLNLLASNPVRSPKLLLTPHPGEAARLLQMTTDEIASDRFAAATKLREKYGAQVLLKGAGSLLVTDRGRCLIDRGSPAMASGGMGDLLTGLIAGLLAQRMAPAQALIAGSLWHAIAGEEAGKDGERGTLASDLLPEAV"
  },
  {
    "reference": "MRNKQKTIKNAVTLSGVGLHTGNKVTLTFKPAPINQGYVFVRKDLEGEPVIEASAEYVINTQRGTNLEKHGVFVNTSEHVLAAVVALDIDNIIIEINAPEPPIMDGSSKHFVEALEKAEIEEQDAERDEFIVKEIISYKDEETGSEIILMPCDNYQITAMVDFGTKVLGTQNATMNALSEFKTEIANARTFSFLHELETLLDNNLIKGGDLNNAIVYVDKEISDETMDKLKKAFNKEKLSVKPNGILDNLTLRWANEAARHKLLDVIGDLALVGCRIRGKVIANKPGHLVNTVFAKKLQKIIKQEKRNCVPKFDLNQPPLMDIHKIMSILPHRPPFLLIDRILELSDRHVVGMKNVTMNESFFVGHFPGAPVMPGVLQVEAMAQCGGVLVLSTVPDPENYLTYFMKMDNVKFKQKVLPGDTLIFSAELISPIRRGICHMQSYGYANGKLVVEAVLMAQIARKPTE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein's active site must be carefully engineered to ensure that the amino acids involved in a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine +   H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate are properly positioned for optimal catalysis.2. The protein design should consider the endogenous proteins and enzymes present in the lipid IV(A) biosynthesis, Glycolipid biosynthesis, lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 2/6 pathway to avoid interference with native metabolic processes.3. A protein with metal ion binding functions is required to enable fatty acid biosynthetic process.4. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects.5. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.6. The protein must exhibit the following characteristics: Involved in unsaturated fatty acids biosynthesis. The designed protein sequence is ",
    "response#1": "MNLLKDILYKVSLEAVVGNTNVAVLETSTDSRALTQGVVFVVIKGQQFDAHDYLDQVVQKGAVAIVVEQQLTLPNGVEYIQVKDSRRALALLANQYYGNPSQKLQLVGIV",
    "response#2": "MSVTQISAALKNIAEFKILDGELDKTIXGVXTDSRKVKKGFIFAALKGHKFDGHDFVXDALSKXAFAVISEHXNFNXPXIIVKNXRKXLSLIASAFYNSPSKKLXVVAXTXGKTNVKSMVAXSLAAXDIKTXVIGTNGXXXNGXIXSNTTPDAFDLQKXLXXXKXXGXXHXXLEVSXHAXXAGXXXGXFDXXVFTNLTQDHLDYHGTFDNYLSAKAKLFTEFLKKDGFAVINIDDXXYKRLLKKCSXSKIFTYSINXPADFYATNIKYSKSGIKGHXXTPXGXFNXQXQLIGKFNVYNILAAGAAAIXCGXSXENIVRGIEKIKXVPXRMEKIGXKENKIWIDYAHTPXAXXAVINSLRTXYKGKLXAIFGCGGDRDKTKRPVMGEIASQYCDXVIVTXDNPRSEDPXKIXADIEXGXXNXSKXIIKNXXXAIXXAXNXSEEDIXILAGKGHETYQEINNIKIHFDDKEXALXSLXEXFN",
    "response#3": "MSQKLQPDPQRTILQSLGINTVPDDLPVAGLPAXAXVXSPRLNRPAXEAXGAXGMPAXAYSTHXHLXLPQRAPLVXEAXRXATLTLAXNIMAXAXRXAXVTXAXAXSXTVXC"
  },
  {
    "reference": "MEIEVVYQHSDFIIINKSEGISVHKDQEEQGLTELVAKQLNVPKVWLVHRLDKVTSGLLILALNAESAAEFSRLFSEHKIHKTYLALSNQKPKKKQGLIIGDMKKAREGAWKLCQTKDNPAITRFESVSCEPNLRLFILKPQTGKTHQLRVAMKSLGSPILGDGLYGKNTEKIDRTYLHATQLEFDYLNNFISVTCLPSQGQFWIKPTVFEQIQVYLTKPFLSK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be able to regulate enzyme-directed rRNA pseudouridine synthesis.2. The protein should be designed to catalyze the following reaction a uridine in RNA = a pseudouridine in RNA. The designed protein sequence is ",
    "response#1": "LIVVNKPAGIAVHGGSGVSAGVIEVFRQLRPDAKDIELVHRLDRDTSGLLMIAKKRSMLRHLHEALRGEKIVDKRYHALVRGHWPAGKKNVRAPLLKNNLRSGERMVEVNVEGKDALTEFRVLRRFGDFATLVEARPITGRTHQIRVHAKHAGHAIAGDPKYGDEDFSREIRELGGKRLFLHAAQLR",
    "response#2": "QLIVVDKPAGVVCQPDAKHELFNLANSLLKHCGYNQYFKNSLSFIPRFAHRIDRNTCGLVIGAKTNQALQELEAVFRHNLLMKQYQGLVFGPFNFKGTQKAYWAKDAYQALVTVKAKPFPNAKPITTIFENSKYLTKLDLSLLTIRLVSGKTHQIRACLL",
    "response#3": "GTPNYFGQQRFGSKRPVTHEVGLAILRDDWEAAAMAYLGAPTEHEPADSQRAREYVAETRDWTGALAEFPQRLRYERTMLHELADGGSFRDAVETFPSNLQQLFVHAAQSYVFNRIVSERMARGLPFGEPVAGDVVWFADSDADAAVAQPDAARQQRVTDSRVDVMARHCERGRAFVTAPLVGTDTEFADGDPGEITRGVLDDLGLSRADFDLPGEFDSAGSRRAILV"
  },
  {
    "reference": "MPAKMLKWLLIHIFLIHSIFCQDAPAQMPFFANSDPMMSKLSQIQFADAPTLKGDKYPDVDAHRIIAPIISPIMEVFDQMKENQRARDQAEKIRKDREDHPLSTSRSLWEMFQRLQRPTTTTTEAPPLIERLFKPYIEPWQKQLDDFSKDMAGITLIPTTTTTPAPTTTTTPNFLEKSLSMFFPSLRRKPQSIPTLPPTTTPTPRLFDPEMFDRLFFKRDKRQATIAPAPKFDIFTVPPPPPLFQEWEKPILSLSNPFTLNPLMKMFTTPSPPQTLAPLPKIPEPNPYEIKKGLPEPQFKLQDPFYNPLFPSRKSKMFDFLAGGEAGRLLG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Acts via the transmembrane protein ptr-23.. The designed protein sequence is ",
    "response#1": "VINTTGLVRPNQGAMLSTQNAGAVSQVLVQNGQNVKKGEVLVELDSSVERANLQAAQAQLSALRQTYQRYVGLLNSNAVSRQEMDNAKAAYDAQVASIESLKAAIERRKIVAPFDGKAGIVKINVGQYVNVGTEIVRVEDTSSMKVDFALSQNDLDKLHIGQRVTATTDARLGETFSARITAIEPAINSSTGLVDVQATFDPEDGHKLLSGMI",
    "response#2": "VINTTGLVRPNQGAMLSTQNAGAVSQVLVQNGQNVKKGEVLVELDSSVERANLQAAQAQLSALRQTYQRYVGLLNSNAVSRQEMDNAKAAYDAQVASIESLKAAIERRKIVAPFDGKAGIVKINVGQYVNVGTEIVRVEDTSSMKVDFALSQNDLDKLHIGQRVTATTDARLGETFSARITAIEPAINSSTGLVDVQATFDPEDGHKLLSGML",
    "response#3": "QSFWLITGIIFIAFNLRPAITSVGPVISSIRAELHMSNGAAGFLTALPLLSFAVLSPLAPKLGQRLGNERTLWLGLVILLIGVLTRSTGYTAALFFGTALIGVGIAIGNVLLPSLIKHKYPEKPGIMISLYTTSMNIFAALASGVSVPLATQMNGGWKQAFLLWGGLALLALLIWIPQLRHRDTANQTMKLQSSSIWASKMAWYVTIFMGLQSFLFYSSIAWFPEILRSHGIDTATAGWMVSLMQFASLPSTFLTPVLADRVKQQRGIVAALASVYLIGLCGLLAGGSHTLLAIWMIIIGIGQGSSISLALTLIGLRSENAQQAAALSGMSQSFGYLLAAVGPIFVGYLFDQTHSWTMPIVLLIAALIVMGAAGQGAGRDRYR"
  },
  {
    "reference": "MAFNFNWSPLMADAGFYTRAQDLLTAALNKSPKPPIIVDDIVVTELNLGSNPPELEILEIGDLAEDRFRGIFKMSYAGDAVLTLKTCVQANPLNTYLLTRHPFTSPQPLAAATGLTIPLQITLSDIKLSGFVILVFSKQKGITVVFRNDPLESLKVSSTFDSIPFVRDYLQKEIEGQLRILFMDELPAIIHRLSLRLWSTEYSELETTCDQVTNPSLEGPGQDPLLNPPQDPVDSFGNVLSISEIASLSLDSGVEMHSLFSRKNALRIAALTDSQRTLSLFTPSIREVVFRAWTGSIEQADGPSGLVSPMSPPLSRTQSHITVASAPLSSQDTASVASSQSRPGLPSSGYSGYGLSLGAARHSKAHARKRKKRVIDLRPHRKPTDDMASISGESSCTDSTITTTTKSAASVYSSSIIPEENNDDDPVTPPVSPVRNSTVRRRQTLRDRIVDRDDAERTNIRRPPIPAEFGHEFPLRPTQPLPSNEIDRNVTSPNPKPEDSHHFQPPRQDTAPIRNIPSAPLGPPAPAPIPASASAYPPEKPTYTQSFPPSFMEVAHNGSILEQAWMMKMASEIARRIQDENSSAMGSYVGSGSGSGGFWDRSHTPPPAYRH",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "NGQQPKPNGNLVVIDLGAVIRNFEDLVLLDINRVGAAIGNSLVQLTAQADVPQEVINIVAQGIAAHVAGAAARQYTRQTGNTLRRITAMDPSKIYARKPNTLVGLARGNADFVDAIHTSAYGLGTTTRAGDVDFYPNGPSVNMPGTDDIIEASLRATRYLAETVLPGNDRNFPAVAAESLQQYKNNNGNGRRAYMGIAADYDLEGDYILQVNAKSPC",
    "response#2": "NGQQPKPNGNLVVIDLGAVIRNFEDLVLLDINRVGAAIGNSLVQLTAQADVPQEVINIVAQGIAAHVAGAAARQYTRQTGNTLRRITAMDPSKIYARKPNTLVGLARGNADFVDAIHTSAYGLGTTTRAGDVDFYPNGPSVNMPGTDDIIEASLRATRYLAETVLPGNDRNFPAVAAESLQQYKNNNGNGRRAYMGIAADYDLEGDYILQVNAKSPQ",
    "response#3": "NGQQPKPNGNLVVIDLGAVIRNFEDLVLLDINRVGAAIGNSLVQLTAQADVPQEVINIVAQGIAAHVAGAAARQYTRQTGNTLRRITAMDPSKIYARKPNTLVGLARGNADFVDAIHTSAYGLGTTTRAGDVDFYPNGPSVNMPGTDDIIEASLRATRYLAETVLPGNDRNFPAVAAESLQQYKNNNGNGRRAYMGIAADYDLEGDYILQVNAKSPC"
  },
  {
    "reference": "MGIPAKSLSQLALTARGGRDPQITGLAVDSRQVKDGFLFAALPGTRVHGGDFIQYALRMGAAAVLTDATGAEIAERELAASGAALVVVEDPRQALACTAALWFGAQPGVMAAVTGTNGKTSVATFLRQIWAGLGHAAINLGTTGVEGAWNAPLNHTTPEPITLHRVLAEAAGHGITHAAMEASSHGLEQRRLDGVQLIAAGFTNFTQDHLDYHESFDAYFAAKAGLFRRVLPPEGTAVINLEDPRGAEMRAIAAARGQAVLTVGRAVGDLRLLAQRFDATGQDLRFSWMGQSHQARLDLIGGFQAENVLIAAGLAIACGDEAGAVFRTLPELTTVRGRMQLAATRENGAAVFVDYAHTPDAVATALTALRPHVMGHLVAIVGAGGDRDRAKRPLMGAAAAEHADLVFVTDDNPRSEDPASIRAAVMAGAPEAIEVGDRAEAILRGIDALGPGDALLIAGKGHETGQIVGDDVLPFDDAEQASVAVAALDGRPV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have ATP binding within cytoplasm to regulate regulation of cell shape, cell cycle, cell wall organization, cell division.2. The ATP binding site should be located in a region of the protein that is accessible to the ligand.3. Incorporating a Meso-diaminopimelate recognition motif motif is critical for the successful design of the protein.4. A protein that can modulate the overall flux of metabolites through the Cell wall biogenesis pathway, enhancing or suppressing its activity as needed.5. The presence of Mg(2+) is necessary for the protein to function in the desired environment.6. The designed protein should contain one or more Mur ligase central, Mur ligase N-terminal catalytic, Mur ligase C-terminal domains that are essential for its function. The designed protein sequence is ",
    "response#1": "VTGTNGKTTVTHLVERIAAQRGHKVGLIGTLGARIAGREIPGQRTTPEATEIQKLLAAMRAEGVSLAVMEVSSHALDLGRVNGCEFDAGIFTNLTQDHLDYHKTMEEYLEAKAKLFRGLQGKKEPKIAIINGDDPAADMLRKASAAETVTYGLGEARDYRAAEIRPGVDGVRFRAEFRGRRQEVFYATPGLFSVYNALAAFALLDAVGVPLDDALAALGAATPVAGRLERAVIAKSGAPVYVDYAHTPDALAAAIAALRPHVTGRLISVFGAGGDRDTGKREPMGRVAVEGSDVVFVTDDNPRGEDPAAIRAAVMQGAKGAREVGDRREAIRAAIAEAGRDDIVLVAGKGHEPYQEIDGVRHPFDDREQARAALARR",
    "response#2": "ITGTNGKTTTSYLIRAILREAGYKVGLIGTIQIMMEEEVFPIHNTTPDVVELQHTLAIMRDKGMDYVVMEVSSHALDQNRVAGIEFDTAVFSNLTQDHLDYHKTLENYKLAKAKLFDLLGQEGVKENKTAVVNADDEAGKTMLEHAQCRHLTYGIDNKASLQATNVEVLASGANFTLTEEFLGGMDLQLHITGIFNVYNVMAAVGAALSLDIAPEAITRGLAAFSGVKGRMNRLGGESGRPLVVIDYAHTPDALEQALASLRGHARARLVCVFGCGGERDRGKRPQMAAIAEAGADLVVVTDDNPRRENGDAIVADIVAGFAHPERAIVLRDRAAAIARAVAEAGADDIVLVAGKGHETYQEIKGVKHHFDDMEEAKAALAAR",
    "response#3": "ITGLTLDSRAVRPGHAFVAIAGFGAHGLKFVAQARAAGATAVLFEPPAPEDLPAPDDAIAVPGLRARLGELGDRFYGRPTRALDLLGVTGTKGKTTVCHLVKSVLDSCGLRTGLIGTVHNIVGDEVRPVTRTTPESSDLQSLMSEMVAKNLDAVTMEVSSHAMELFRVEDILFDAAVFTNMGWDHLDFHGTMEAYAGAKARLFSLLERSRGLKKSERLQTAAINADDPYAGLFRSKAVGRVVTYGLGEDSEVRAKDVRMDARGTTFTLVAGDIERRVRTVLLGRFNVYNSLAAAAVAMHLGVAASAIEAGLAAAPHVPGRMQKAATRENGATVFVDYAHTPDALVTALQAMRPHVMGRLIVVFGAGGDRDTGKRPLMGAAARDHADVVFVTDDNPRSEDPAAIRAAVMEAVPDATEVGDRAEAILRGVDALGPGDTLLIAGKGHETYQIVGDERLHFSDVEVAREALRRRG"
  },
  {
    "reference": "MTTNSNLEALSDAGVSIWLDDLSRKRINSGNLAELISDYAVVGVTSNPTIFAKALSNAADYDEQVRELAARGASVDDAVRELTTRDIREAADIFRNIYEAGHDGRVSLEVDPRLAHDTERTVAEALDLWKAVDRPNLMVKIPATVEGLPAITRVLAEGVSVNVTLIFSVERYRDVMDAYLAGLEQAKANGHDLARIASVASFFVSRVDTEIDKRLESVEGGQELRGKAAIANARLAYAAYEEVFASDRFKALAAEGGKPQRPLWASTGVKDPSYSDTRYVDELVAPNTVNTMPEATLFASADHADVRGDQVSGKAAESQQVFDSLSAAGIDLDDVFAVLEREGVDKFEKSWEELLQTVREQLDQAK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.2. A protein should be able to manipulate the activity of enzymes in the D- glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D- ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3, Carbohydrate degradation, pentose phosphate pathway pathway, either by activating or inhibiting their function.3. The Schiff-base intermediate with substrate of the protein must be engineered to enable effective catalysis of D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate =   beta-D-fructose 6-phosphate + D-erythrose 4-phosphate.4. The protein should be optimized for stability and expression in cytoplasm where it will participate in carbohydrate metabolic process, pentose-phosphate shunt. The designed protein sequence is ",
    "response#1": "MAKRIDLNAKLGFGIVSAPPRSGKTTLARALGLPVFELDEIARHEGWKPGEVQGEKLRRLANEAARREAWIIDGNYSRTLDLPVSRAVWLDYPRQVCIWRSVYRRIVRTVALGRPIAPGCADSVPESFLHYVWSWDNTYRPLILETFRRHAKDGGIIEFRTRAQVDQFLKRLEQQDRQQR",
    "response#2": "MNNETEFDSIEEAIEDIKKGKIIIVVDDEDRENEGDFICAAEFATPESVNFMATHGRGLICVPLTEERCEELELDLMVSKNTSLHETPFTVSVDLLGQGCTTGISASDRAKTIQALADEKSKPEDLGRPGHIFPLRAREGGVLRRAGHTEAAVDLAVLAGLYPAGVLVEIMNEDGTMARLPDLMKIADKFNLKIISIKDLIAYRLKQEKLIEREI",
    "response#3": "MVDPRPALLFDLDGTLVDSERLHRQAWERAALEAGLELPWERVLAVIGVSGPAIADRLVAEHGEDWLVARRTEKHALYREEVAVRPGAAELVAGVREAGIPLALVTSSSREMVERNVAALLPEAVVVDGASDRGKPDPEPYLLACERLGVDPGDCLAFEDSTSGVTAAKAAGMRCVAVPHVVPVDEDLAGADVVVDHFTEVRLADLRRLLGATEDGPAGDVAVGDD"
  },
  {
    "reference": "MKNQINLSPIKSTKPIPLYSSEQLYAMEQAWFVEGHDSFGLMKQAAWQMAQHIEQLYEQKQLNIYPSATTYVPRQHRVSIWVGKGNNGGDGWLIAYYLQQMGWQVQVITVGFESDDFDTSNITVFSDAKKALKEALLANCPYRRFENSACDLEEGIDGNLQAEVYIDALFGIGLDRAPTGIYKTAISTFNELSKGNNTLAIAVDIPSGLVASTGEVFEHVAIQADITLCLIARKFGLHTKDGMDHSGKVIDIPLIPYPINDKRFIPVATLITTAYGLSPRRQNSYKGSYGHVLIIGGNRIDGSQGMGGAAILSASSSMAAGAGKITVACHEAFHSALLTSLPDAMTINLHDVDGVQELIKSATVIAIGMGLGRDERAKVLFIKYLQTAMTAKKAIVIDADGLYHLASLQLEHHELVSQLREYSVNHQVCLTPHSGETARLLNKRISEVESNRLQAIKQCATTYGGEWVLKGAGSLILEQGLDEHHVYVCAAGNAGMATAGMGDVLSGVIAGLLAQQDLSSGMQSLHQAVVIHGLAGDTLVNQSNNTLLVGQRGLQAQDMPAAIRHMMQLLINVYN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be optimized for ADP-dependent NAD(P)H-hydrate dehydratase activity.2. The protein must exhibit the following characteristics: Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.. The designed protein sequence is ",
    "response#1": "LLVVAGSEGMTGAAALAGEAAMRVGSGLVSVAVPQSLRTVIEMKLTECLKLGLEESEPGVLGEGAVRQLTPHLHRYHATLLGPGLSTRGEVARVVVALIERAEHPMVLDADALNILAGTLESLEVAKAPLILTPHPGEMARLLRTSTARVQENRLSIAHDFATRHGVVLVLKGARTLIATPEGEVFVNPTGNAGMASGGTGDVLAGMIGGFLVQGHPPRTAALLGVYLHGLAGDLAAEALGERSFIASDLLDRVMVIGGSSEFVGAPWLAALSSLYGGVDLVYLAAPAPVLSSIISPEIIPIKLHGERLVEAHVERLVSEIVSRRVHVLVLGPGAGRCEETINFFCKLYEKLTHLEHVSLLGIVVDADALYSSAQCLGHLQEVGRRYNVILTPHLGELKRLLGREIADTLEQRIEASKEAAKKTNSVVLLKGAIDVIASPNGKIKLNRTGTAQMTVGGTGDVLAGLVAAFLARGANPFYAAALAAFVNGLAGERVVKNVGRVTPMLLIEWLRH",
    "response#2": "RAGHKYDHGHAVILSGPMGRTGAARLAARAALRIGAGLVTLAAPGSAMMECAAQLTAIMLRRCDDAGALAEILSDERLNAVCLGPGMGAGEETVARVSAALRMAERGARGVVLDADALSAFQADPDALFALTANASRTILTPHFGEFQRLFPDLARHLSGTPETGPAYSRLDAARAAAKRAGCVVLLKGADTVIAAPDGTARIHAALYDRAAPWLATAGAGDVLSGLIAGLLARGFAPLDAACMAAWLHVEAARHFGPGLIAEDLPDALKYTRGVVGVVAGSARYTGAAALSVEAASVSGPGMVRYLGPETVARQVLARTPEAIWEPDEPGRVQAWLLGPGLDGSDQLQRAKDALQAAYDGGQPAVVDAGALELLPERCPPNWILTPHAGELTSLLGRSEVRVTRDDVESRTLHFVRLAAEHTGAVVLLKGATTLIASPSGTVFSQSDGNPWMSTAGSGDVLGGILGALAASYAASGLGDDGPFAGIGIGPEERWAAVAAVGASIHGMAGKAASGAGPTTAAAIAREVL",
    "response#3": "LVVGGEGWKSGAPRLAAGAALRAGAGLVTIAAPFRALPIYASQLTAVMLAEANNPDGLGDVLADERKNAVLLGPGLGVGAGTAAMVKVALCGRRAIVLDADALTSFEARREDLFAMIAAEAGRDVVLTPHEGEFARLFGKETLTEAPSKLEAARAAARASGAIVVLKGSDTVVAGPDGRAAVNEMSSPFLATAGTGDVLAGIIVGQLAQGVAGFEAAASAVWIHGAAAAVGVVAGSARYTGAAALSVEAASVSGPGMVRYLGPETVARQVLARTPEAIWEPDEPGRVQAWLLGPGLDGSDQLQRAKDALQAAYDGGQPAVVDAGALELLPERCPPNWILTPHAGELTSLLGRSEVRVTRDDVESRTLHFVRLAAEHTGAVVLLKGATTLIASPSGTVFSQSDGNPWMSTAGSGDVLGGILGALAASYAASGLGDDGPFAGIGIGPEERWAAVAAVGASIHGMAGKAR"
  },
  {
    "reference": "MSLRGSLSRLLQTRVHSILKKSVHSVAVIGAPFSQGQKRKGVEHGPAAIREAGLMKRLSSLGCHLKDFGDLSFTPVPKDDLYNNLIVNPRSVGLANQELAEVVSRAVSDGYSCVTLGGDHSLAIGTISGHARHCPDLCVVWVDAHADINTPLTTSSGNLHGQPVSFLLRELQDKVPQLPGFSWIKPCISSASIVYIGLRDVDPPEHFILKNYDIQYFSMRDIDRLGIQKVMERTFDLLIGKRQRPIHLSFDIDAFDPTLAPATGTPVVGGLTYREGMYIAEEIHNTGLLSALDLVEVNPQLATSEEEAKTTANLAVDVIASSFGQTREGGHIVYDQLPTPSSPDESENQARVRI",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Seems to be involved in negative regulation of the survival capacity of activated CD4(+) and CD8(+) T cells; May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis; May contribute to the immune evasion of H.pylori by restricting M1 macrophage activation and polyamine metabolism .2. Ideal protein should have a binding affinity for key enzymes in the urea cycle pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "GGIDSHIHFICPQQIEEALASGITTMLGGGTGPATGTFATTATPGPWHIERMLQAADAFPMNLGFLGKGNASLPDALHEQIEAGVMGLKLHEDWGTTPSAISNCLDVADATDTQVAIHSDTLNESGFVENTIAAVGGRSICAFHTEGAGGGHAPDILRVVGEENFLPSSTNPTMPYTVNTLDEHVDMLMVCHHLDAGIAEDLAFAESRIRKETIAAEDVLHDLGAISMFSSDSQAMGRVGEVVLRCWQTAHKMKLQRGKLPEDSERNDNFRVKRYVAKYTINPAISHGIAHEVGSIEVGKWADLVIWKPAFFGVKPFTLIKGGTIAMAAMGDPNASIPTPQPVHYRPMFGSYGGSLARSSLTFVSQAGLAAGIKERYGLAKHLSAVKNIRNVRKKDLVHNGYTPKMEIDAQTYAVRADGHLLTCDPAVQLPLTQRYFLSAADY",
    "response#2": "GGIDSHIHFICPQQIEEALASGITTMLGGGTGPATGTFATTATPGPWHIERMLQAADAFPMNLGFLGKGNASLPDALHEQIEAGVMGLKLHEDWGTTPSAISNCLDVADATDTQVAIHSDTLNESGFVENTIAAVGGRSICAFHTEGAGGGHAPDILRVVGEENFLPSSTNPTMPYTVNTLDEHVDMLMVCHHLDAGIAEDLAFAESRIRKETIAAEDVLHDLGAISMFSSDSQAMGRVGEVVLRCWQTAHKMKLQRGKLPEDSERNDNFRVKRYVAKYTINPAISHGIAHEVGSIEVGKWADLVIWKPAFFGVKPFTLIKGGTIAMAAMGDPNASIPTPQPVHYRPMFGSYGGSLARSSLTFVSQAGLAAGIKERYGLAKHLSAVKNIRNVRKKDLVHNGYTPKMEIDAQTYAVRADGHLLTCDPAVQLPLTQRYFLSAADY",
    "response#3": "GGIDSHIHFICPQQIEEALASGITTMLGGGTGPATGTFATTATPGPWHIERMLQAADAFPMNLGFLGKGNASLPDALHEQIEAGVMGLKLHEDWGTTPSAISNCLDVADATDTQVAIHSDTLNESGFVENTIAAVGGRSICAFHTEGAGGGHAPDILRVVGEENFLPSSTNPTMPYTVNTLDEHVDMLMVCHHLDAGIAEDLAFAESRIRKETIAAEDVLHDLGAISMFSSDSQAMGRVGEVVLRCWQTAHKMKLQRGKLPEDSERNDNFRVKRYVAKYTINPAISHGIAHEVGSIEVGKWADLVIWKPAFFGVKPFTLIKGGTIAMAAMGDPNASIPTPQPVHYRPMFGSYGGSLARSSLTFVSQAGLAAGIKERYGLAKHLSAVKNIRNVRKKDLVHNGYTPKMEIDAQTYAVRADGHLLTCDPAVQLPLTQRYFLSAADY"
  },
  {
    "reference": "MLALVVALASHRGIGNANALPWPRPLAADMAWFRTLSQSIPLISPDRIALAPSASNAVVMGRRTWDSIPSRFRPLANRINVVLSRGPARSTENTFFIQTFEALDSLPLPPSSMTFVIGGRDVYSLALESGRPHLIFATEVFESPECDVFFPHIDWASYEKRDITRDVSRLIDRTLASAFYSPETATFTENGTSFKMFLYTKPETR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be designed to catalyze the following reaction (6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate +   H(+) + NADPH. The designed protein sequence is ",
    "response#1": "MIISIWAMTKEGLIGKDNQMPWHIKEEFQHFRRQTLHKTLIMGKNTFFSLPKVFDKRKMYVLSHEKDFKIDHPDVEVIYDYKDLLKEYANNDKKDIYVAGGLYVYQQLIPLSDILIVSIVKGSYEGNKYLSVDFSNFVQYKQPEIYDQFSVYYYKKKDQKNSK",
    "response#2": "ISLISAISNNLVIGHNNKIPWYLPEDLKWFKKNTIHKDIIMGRLTWESILNHPLPMRKNIVISHKEIIHKDVIWANSISKALLSTTYDKEIMVIGGSKIYKQMLFYATKLYLTHVDFNGIGDTYFPQYKSCKFWKTIFRKQHFKDKNNPYNA",
    "response#3": "MIISIWAMTKEGLIGKDNQMPWHIKEEFQHFRRQTLHKTLIMGKNTFFSLPKVFDKRKMYVLSHEKDFKIDHPDVEVIYDYKDLLKEYANNDKKDIYVAGGLYVYQQLIPLSDILIVSIVKGSYEGNKYLSVDFSNFVQYKQPQ"
  },
  {
    "reference": "MILKDSTINNYATALFNIAVKEKLVDDYIIQVDALIQSLSDKDDFNKLVSFSNKQEKQDAILIIEKTFAPFEFDIYLINALKILVENQLFINTRMILKALYKKLLDYKNIVLGVVYSTEKLTKTQLSAIKKKISNKVNKKVELVNKIDPTLIGGIKVNVQGKVFDGSIKAKLEALKKQMNT",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: It either transmits conformational changes from CF(0) to CF(1) or is implicated in proton conduction..2. The designed protein should have a specific function within the proton-transporting ATP synthase complex, catalytic core F(1). The designed protein sequence is ",
    "response#1": "SCGGE",
    "response#2": "MG",
    "response#3": "SQI"
  },
  {
    "reference": "MSTLLLEAAPNTVLGNIIVVSGAFIILLVLLRLFAWNAITSVFASRAKKISDDIDAAEANNKQAADLVKQRQAELAGSKEEAANIIQVANDTASQNRAKVLATANEEATSLKKRAQEDIEQERKEALNTVKGDVADISVQIAEKLIGQSLDASAQQELIDSYLAKLGE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have proton-transporting ATP synthase activity, rotational mechanism and be active in plasma membrane.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. For general function, the protein need meet that Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0). The designed protein sequence is ",
    "response#1": "MXXXR",
    "response#2": "MTRKL",
    "response#3": "SLLG"
  },
  {
    "reference": "MGRLLARRLGRPLLDIDALVVQEAGASIPELFTRHGEAAFRDLESRVLEQAVCSEESAVIATGGGIVVREENRRLMAERGVRLYLHTDPTSSLERLLAQQERARADLTSPEVRPLLSGPDPLQTLQNLYMSRRAWYEEAELTCATQGRSAEQVVEEIIAMLTDAEASHETAPIVRRVHIGAGYDIVVDWGGLERLPQYLTQLALPRRVFLFTDSNLRSLYGDDVIQNLARAGFDAYLYTVPAGESSKSFEQLQAIYDWLLEHHAERREPLIALGGGVIGDLVGFVAATYLRGVPLIQVPTSLLSQVDAAIGGKTGINHSQGKNLIGAFYHPLLVLVDPATLRTLPLRERTEGWAEVVKYGIILDAKLFALLETYKDALGDFSSLPASLLMQIIARSIDLKVQVIEEDEKESGLRAILNYGHTVAHALESVAGYGELLHGEAVSLGMVAAAALAQEAGMLPSDEVQRQNALLEALGLPVAYQGNVRAQAILAKIQLDKKVVSKRVRWIMPRHIGDVVITEMPDELVERVISTFFAEARV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should take into account the physiological conditions of the Metabolic intermediate biosynthesis pathway. The designed protein sequence is ",
    "response#1": "LSETLKEQSWFSTPRSAWEKVKAVRQVERPSASDYISHIFDFFVESHGDRAFSDDKAMIGGLAFLDGQPVTIIADEKGKDFKECQERNFGMPMPEGYRKALRLMKQAEKFNRPVISFVNTSGAFCGIEAEERGQGEAIARNLMEMSSLKVPVLCLMIGEGGSGGALATAVGNEVWMMENATYSILSPEGFASILWKDANRAKEASEVMNITAQDLKRLGVIEEIIPEFGGADHKTTEAIAGYMKEHIKDFLRR",
    "response#2": "LSETLKEQSWFSTPRSAWEKVKAVRQVERPSASDYISHIFDFFVESHGDRAFSDDKAMIGGLAFLDGQPVTIIADEKGKDFKECQERNFGMPMPEGYRKALRLMKQAEKFNRPVISFVNTSGAFCGIEAEERGQGEAIARNLMEMSSLKVPVLCLMIGEGGSGGALATAVGNEVWMMENATYSILSPEGFASILWKDANRAKEASEVMNITAQDLKRLGVIEEIIPEFGGADHKTTEAIAGYMKEHIKDFLRG",
    "response#3": "GNAVATILATLGTDVADPVQRLAAVTASTRAAKAQLRSMDKDAILAYSAALMAPYGVQLASTLSGVKPPWPYTFNLCVSNVPGPEDVLYLRGSRMEASYPVSLVAHSQALNVTLQSYAGTLNFGFIGCRDTLPHLQRLAVYTGEALDQC"
  },
  {
    "reference": "MPIPGHIDPVPVPRAMPARADGRIDLLGMSKESVREALEAAGWDARQAKLRAKQLWHWIYNRGVTDFTKMTDIAKAQHPWLEERFVISRPDVVEAQVSTDGTRKWLLRSPDGQDYEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNLTAGEIVGQVMLARDALGEWPSQPEGRMLTNIVMMGMGEPLYNFENVKLALKLVMDGDGLALSKRRITLSTSGVVPMMERCGEEIGVNLAVSLHATNKDVRDEIVPLNKKYGIEDVLQACADYPGANNARRITFEYVMLKDKNDSDDDARELVRLLRKYRLPAKVNLIPFNPWAGAPYECSDPDRIRAFSNIVFEAGISAPVRTPRGRDIDAACGQLKTASEKKSRAELDRMAAQAAVQEA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The [4Fe-4S] cluster, S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand.2. The designed protein is the optimization of the S-methylcysteine intermediate, Proton acceptor to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.3. The presence of Radical SAM core domains is critical for the interaction of the designed protein with its intended targets.4. The designed protein should be involved in rRNA base methylation by localizing to cytoplasm and exhibiting 4 iron, 4 sulfur cluster binding, tRNA (adenine-C2-)-methyltransferase activity, tRNA binding, rRNA binding, rRNA (adenine-C2-)-methyltransferase activity.5. The protein must bind to [4Fe-4S] cluster in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MTACISSQVGCSLTCAFCATGKLERLRNIDADEIFDQVALIKKQAEEHYKTPLTNIVYMGMGEPLLNYSNVLKSVELISSEEGLGMSPKRITVSTAGIAKMIMKLGDDQVKFNLALSLHAATDEQRSKIMPINETNSLEALGEALQYYHEKTGARVTFEYIIFKDFNDSLQDARNLAKFCKKVPCKINIIEYNPIDNAQFEKADSKKTDEFAQFLEQERRDKDAAAEKVDVRSLDYKELQEEMTRIGEKAFRGKQIYEWLHVKLADSFQEMTNLSRSLRERLEAQYVIRKVDMLEHQISKKDPTEKFLFELEDGNVVESVLMKYNYGNSVCISSQVGCRMGCKFCASTIGGKVRNLTTSEMLGQVYRIQKISGERVSNIVVMGTGEPLDNYDNFLKFIHMVSDEHGLHISQRNITASTCGIVPNIKRLAEEKLQITLALSLHGSSQEKRQELMPIAYQYDLSEVLAACEEYFEKTGRRITFEYSLVQGVNDTAEDAARLIKMLGHMNCHMNLIPVNPIKERAFQKPSRKNAEEFKNKLEHSGINVTIRRERGSDIDGACGQLRRRHITSKRTKKD",
    "response#2": "MTACVSSQVGCSLTCKFCATGYMERKRNLNPDEIYDQVVLINQQAMENYGAPLSNIVYMGMGEPLLNYKNVMESIDRITSEDGLNMAAKRITVSTAGIAKMIKKLGDDNVKFNLALSLHAANDEKRNTIMPINEQNSLKALAEALKYYYAKTKNPVTYEYIIFDGVNDNIQDAVELAQFCKHLPCKVNIIEYNPISFANFINAGEDKVEAFADYLRDTPVDASYDGDRATLCVSSQVGCALNCHFCSTAQQGFNRNLTVSDIIGQVWRAAQIIGPSAVSETRPITNVVMMGMGEPLLNLSNLIPALKIMTDDFGYGLSKRRVTVSTSGVVPALDKLGDEIDVALAISLHAPNDKLRSEIMPINDKYNIEAFLAGVERYISKSNANRGRVTVEYVLLDHVNDSMDQAHELAQTLKNTPCKINLIPFNPYPGSPFMKPSNSRIDRFSKVLMEYDLTVVVRKTRGDDQVSACAGTPQRQSWGNSVCVTTQVGCRMGCTFCASTVGGLVRNLTAGEIVDQIVMMQRELPQGERISTVVLMGSGEPLENYDHVLKAVRLVHDPEGLNIGYRHITISTSGIVPGMRRLAEEGLPITLALSLHAPTDELRRQLMPVARIWPLAEVLAAAREYGEKTGRRVTYEYILIEGVNDGPEEARQLARLLKGALAHVNLIPMNPVAERPQYRRPGPERVNRFKEILESNGIATTVRREMGGQRS",
    "response#3": "MTACVSSQVGCSLTCKFCATGYMERKRNLNPDEIYDQVVLINQQAMENYGAPLSNIVYMGMGEPLLNYKNVMESIDRITSEDGLNMAAKRITVSTAGIAKMIKKLGDDNVKFNLALSLHAANDEKRNTIMPINEQNSLKALAEALKYYYAKTKNPVTYEYIIFDGVNDNIQDAVELAQFCKHLPCKVNIIEYNPISFANFINAGEDKVEAFADYLRSREELYGNVEKVDVRSLDYKELQEEMTRIGEKAFRGKQIYEWLHVKLADSFQEMTNLSRSLRERLEAQYVIRKVDMLEHQISKKDPTEKFLFELEDGNVVESVLMKYNYGNSVCISSQVGCRMGCKFCASTIGGKVRNLTTSEMLGQVYRIQKISGERVSNIVVMGTGEPLDNYDNFLKFIHMVSDEHGLHISQRNITASTCGIVPNIKRLAEEKLQITLALSLHGSSQEKRQELMPIAYQYDLSEVLAACEEYFEKTGRRITFEYSLVQGVNDTAEDAARLIKMLGHMNCHMNLIPVNPIKERAFQKPSRKNAEEFKNKLEHSGINVTIRRERGSDIDGACGQLRRRHITSKP"
  },
  {
    "reference": "MILGVGTDILEILRMERLQDSSAAAEKIFTEEERRQSEGKVSRLAGDFSVKEAVSKALGTGIRGFSLLDIEVLRDELGKPYVKLYGNAKKIFLRLGGSRLEVSISNTATLVIATAVLFGNSFSFSEGITRDSSLALESGEGEKDNVFFEETERNSEANSKRNKTLQWLLPLPERNAESHKGSYGKVEIFAGKSGMAGAAIFSALSAYKAGAGLVKVISDKENQIPLQSMVPESIFLDKEKFSLEESLNEGQVLLFGPGIGMGEKEKEFLHELLLRGKTAKDKRQFLILDADALNMISKSSLLEDALRIYAKEAPLILTPHIKEFARLCHLTVEEVLLHRKDLGKKYADSHHCILILKSHDTMVFAPSLDSPEGFLGKREIEKPSRAGEKKTEDKENKKEEDKERKWFFHNTISCAALSKGGSGDCFAGLLSGLLCILEEKYGNFPAKVLAFQAACLSVLVQVRGAEIAAHQEGQHGVLARELPRFFSLAIEEFIEKEDKENAR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must bind to Mg(2+) in order to perform its enzymatic function.2. The protein design must be focused on enhancing the efficiency of fatty acid biosynthetic process. The designed protein sequence is ",
    "response#1": "WGDQLVAIYAADVLKSHPGATIIADVKASQTLFDEIARLGGNPLMWKTGHSLLKAKMAETGSPLAGEMSGHIFFADKWYGFDDALYCAVRLIGLVSKLNQPLSELRDRLV",
    "response#2": "MGTDIVAVERMARLHARFGERLTERLLGPLEVADMPVEAAAGAAFLARRFAAKEATFKALGSGMTNGMRWMDVQVGHDSGGRPQLVLGGRAQA",
    "response#3": "MGTDIVAVERMARLHARFGERLTERLLGPLEVADMPVEAAAGAAFLARRFAAKEATFKALGSGMTNGMRWMDVQVGHDSGGRPQLVLGGRAQA"
  },
  {
    "reference": "MENRWQVMIVWQVDRMRIKTWHSLVKHHMYISRKAKGWIYKHHYENMHPRVGSEVHIPLGDAKLVIMTYWGLHTGERDWHLGQGVSVEWRKERYSTQVDPGLADQLIHMYYFDCFSESAIRNAILGHIVSPRCEYQAGHSKVGSLQYLALTALIKPKKRKPPLPSVAKLTEDKWNEPQRTKGHRGSHTMNGH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that can perform RNA binding in host cell cytoplasm, cytoplasm to support viral life cycle.2. The protein must exhibit the following characteristics: In turn, they are directed to the 26S proteasome for degradation; May interact directly with APOBEC3G mRNA in order to inhibit its translation; Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism; Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions; Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G.3. The protein design must feature the BC-box-like motif, HCCH motif motif. The designed protein sequence is ",
    "response#1": "GWSVHKP",
    "response#2": "MEPVDPSLEPWNHPGSQPKTACNKCYCKKCCWHCQICFLKKGLGISYGRKKRRHRRGTPQSSKDHQNPVPKQPSSQPRGDPTGPKESKKKVESKTETDRFD",
    "response#3": "MEPVDPNLEPWNHPGSQPTTPCSKCYCKKCCWHCQICFLKKGLGISYGRKKRKHRRGTSQSSKDHQNPVPEQPLPTTRGNPTGPKESKKKVESKTETDQFD"
  },
  {
    "reference": "MGDPKKPRKKWEGPRHPWRKEVLVQELKLLGTYGLRNKRELWRAQTIVRKFRHQARSLLAAPQEIREQAEKALLNRLYRLGLLHENASLEDVLGLTVEDLLERRLQTIVYKKGLARTIYHARQLIIHGHIAIAGRRITSPGYIVSREEEDLVDYAPTSPFKKSIEEKA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein contains novel S4 RNA-binding domains that confer a unique function or activity.2. The designed protein should be optimized for structural constituent of ribosome activity in order to improve translation. The designed protein sequence is ",
    "response#1": "MRLDNVVYRLGYGASRTEARQLVNHGHFLVNGKKVDIASFKVSVNDVITVCEKSRGSERFV",
    "response#2": "TLYVGNLPKNCRKQDLRDLFEPNYGKITINMLKKKPLKKPLKRFAFIEFQEGVNLKKVKEKMNGKIFMNEKIVIG",
    "response#3": "RRLDNVVYRLGLAESRAQARQLVRHNFFTVNGRKVNIPSYLVKVGDVIALKEEKKDSPIAKEL"
  },
  {
    "reference": "MNLIQLLQAILPATILSQLDHTLEIKGLVTDNRDVEDSDCFIALAGITHHGKQFIDDAVRRGACVVLVESDDDGIEFKVNQQKQKIPVISLSNLKANVNALLIRFYDVSSSAFDMRLFGVTGTNGKSSITRFVAQMSADLGQSAGLMGTLGFGVWPDVIESKNTTPELAVLLRQFSAMQKAGAQVVAMEVSSHGIEQQRIEGLTFDSAVFANLSQDHLDYHGDMESYFAIKRKLFLPESLQNAIINIDDVYGQRLLADDAITAQKISYGFSEQADIRVVNWAMHATSIDADIVTPWGDASFTINMVGDFNLANVLAAIGLLCVENKFSFQEIITSIQSITPAPGRMQTYCKQGSVPVVVDFAHTPDALENVLSTLSKQVEGQLAVVFGCGGNRDAAKRPLMASVAQNLADVICFTADNPRDENLESIINDMQAGLLVSNNALVQVEHDRQQAIVQTINILTENDILLVAGKGHEQYQEIAGAKNTYSDEAVLFSLGYQDSSSLQDSLLLKNKTALDKEALL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have ATP binding to facilitate cell division.2. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan. The designed protein sequence is ",
    "response#1": "VTGTNGKTSSTYMLRDILRNAGYKVGVIGTIKIMIEDEELPIHNTTPDVIDLQETLAKMYEKGMDYVVMEVSSHALDMNRVAGCEYDTAIFTNLTQDHLDYHKTMENYALAKAKLFDSLSAPDLVKTGKTAVINIDDELGSKTMIEHTKCDLITYGIKHKDATLKAENVEIKASGASFEVEYNNEVMHFDLKVTGMFNVYNILGVIGAE",
    "response#2": "VTGTNGKTTTTHLIEFLLQEAGKSPALFGTLYARWQGFEQVATHTTPFSVHLQEKLAQARNAGAEVGVMEVSSHALAQSRVLGCEFEVAAFTNLTQDHLDYHENMDEYFAAKALLFSPQYLKGRAVVNVGDAYGQLLADQIPGDRLWTYACEDTSADLYTENLTYTPTGVSGTIQSPQGSAAFSLPLVGQYNLENMLAAVGTAF",
    "response#3": "VTGTNGKTSVATFLRMIWQELGLPAANLGTTGVEGDFEAPLKHTTPEPITLHRTLRDATQAGVECVAMEASSHGLEQRRLDGVQLTAAGFTNFTQDHLDYHATFDDYFDAKAGLFARVLPEDGTAVINIDDMRGTDMAAIAKGRGQHVITVGRHGADLELLAQRFDATGQDIRFSFGGEIYQTRLNLIGGFQAENVLLAAGLE"
  },
  {
    "reference": "MWRIMMKSKLHRATVTAADLNYIGSITIDSDLMAAADILPNEKVQVVDNNNGARLETYAIPGPAGSGVICANGAAARLVQPGDIIIIIAYGIFDDREARTYQPRVIFLDARNKITAVKKGEQPGQVWV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The presence of pyruvate is crucial for the protein to function optimally under specific conditions.2. A protein should be able to manipulate the activity of enzymes in the Cofactor biosynthesis, beta- alanine from L-aspartate: step 1/1 pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "LKPIMDKFGVEMVNITTMQAVSGAGYWGVPSMAILDNIIPYIKNEEEKMESESLKILGDLKNGKFHYASFKIGVSCNRVPVIDGHLESIFVKTVEDAEPEEIVKVMDNFDPLKDYNLPTYAKPIVIRKEIDRPQPRLDRDNGRGMSITVGRIRRDNIFTVKYTALEHNTG",
    "response#2": "LKPLHDAASIKRVVVSTYQAVSGSGQKAITELETQVRKMFNMEDPEASVYPHRIAFNCLPQIDVFLDNDYTKEEMKMVNETVKIMGDHNIKVTATTVRVPVFYGHSESVNIEFENKLTAKEARVILTQAPGITVLDNPKEGIYPMPINSAGEDETFVGRIREDETIDNGLNLWIVSDNIRG",
    "response#3": "LKPIMDKFGVEMVNITTMQAVSGAGYWGVPSMAILDNIIPYIKNEEEKMESESLKILGDLKNGKFHYASFKIGVSCNRVPVIDGHLESIFVKTVEDAEPEEIVKVMDNFDPLKDYNLPTYAKPIVIRKEIDRPQPRLDRDNGRGMSITVGRIRRDNIFTVKYTALEHNTG"
  },
  {
    "reference": "MPKEDDSLCKIVVRREPLDVLLPYYDASETTVQKILHENDSTLSVKFLAGVEALIKKDELDKYKNGKACLRVWLKHKSRKRYHGYMTSTDKDEEEKNDYLLKSNGSKVLRDSTRTKKFKFGKEFHCALNPSFVSDETASDSATSSSSDTNKKVNRKEHNELSLSHLSFNDTSDFGSSDLSSSEIESTENDNKAPYFSLLYSDGFDFIKFLHVCVCVKCHGREHRSSGKNFVYCDHCSNVYHYDCSPLPSLNKETRNYSQQNGFICPLCSKNSKDVLCNTGFVSGVSSGQDLVIPPSLADRESLSILVNYCKSIRFRCFRCRRVEYFFYLDSNPLSIQRTITHFIKKLVCNECSMHPCDIEEIIAWRTLNSLYPSKATLSNNFVSTSDLSFWSREYFVRSKGKSYLHCFWCSASWLAGISLAKKKNFDGLENASYDATKPIIPVSYTIIDKVWDVQYRSGKNARTAKYKTKKHQLKAISEVTFAFVSWRGLTYYMSNWEPPPKETDRNRWKAWLKGYSDLLECLWIEKAPTASINIDLPFTNLEWHSQPSFIKGGTLMPYQLKGLNWLYLRWYTHHPCILADEMGLGKTVQVISFISVLFYRHKCFPVLVIVPHATVANWERELKKWAPFLQINVLVGSEKNRSLVRDYRLINQKDPKHVSTHVLVISASNVEREISLLRKFQWKVLIVDEGQRLKNDQSSLFYYLSSVKSDFKLLLTGTPLQNNVRELFNLLQFLNPMKINAAELEKRYSIIDTEKVTELHQILKPFF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The target protein must exhibit strand-helix-helix-turn-strand-strand-turn-strand-helix-helix-helix-helix-strand-helix-strand as its primary conformation.2. The designed protein have Helicase ATP-binding, Helicase C-terminal domains with specific sequence motifs that are crucial for its activity.3. The designed protein should be involved in chromatin organization, nucleosome organization, chromatin remodeling, heterochromatin formation by localizing to SHREC complex, pericentric heterochromatin, heterochromatin island, chromatin and exhibiting nucleosome binding, ATP hydrolysis activity, ATP binding, helicase activity, DNA binding.4. The protein must exhibit the following characteristics: Required for proper positioning of nucleosomes at heterochromatic loci and for transcriptional gene silencing (TGS) function of the Snf2/Hdac-containing repressor complex (SHREC)..5. The ATP binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MDHSIEVTFRVKTQQVIIPEQNIRGNELPLRRWQMELLMLDATGKEVEPTILSKCIYHLHSSFKQPKRRLNSLPFFIKETGWGEFNLKIECFFIGNAGKFSIEHDLTFEDDAYAVDYTVDVPHEFSHLNSELSKYFDLHQVEGVSWLIQKYLLGVNVVLELDQMGLGKTLQAISFLSYLKFRQGLPGPFLVLCPLSVTDGWVSEINRFTPNLEVLRYVGDKYCRLDMRKSMYDHVKKSSKGHFLPFDVLLTTYDIALVDQDFLSQIPWQYAIIDEAQRLKNPNSVLYNVLLEQFLIPRRLLITGTPIQNNLTELWALMHFCMPLVFGTLDQFLSAFKETGDGLSGLDVSNDKETYKSLKFILGAFMLRRTKSLLIESGNLVLPPLTELTVMVPLVSLQKKIYTSILRKELPGLLELSSGGSNHTSLQNIVIQLRKACSHPYFAWINIVRRDIAKHHRIFTTFHRKLSIDAKRFADGCQREVRMKVGRSYKIPRTAPIRTRKISRDMLLFWKRYDKQMAEERKKQEKEAAEAFKREQEQRESKRQQQRLNFLIKQTELYSHFMQNKTDDVEIHLPFLANTAQERLMQWYHQGLNAFECTCPSGHILFNEFSGELLESLLNNDEAVLESLLDGTKARYQELKVAMEQGRDKLLEINSHGGERANQLVKTLADKDEDTQLIGSVIRLWDIIGVEQEDSGENAIVLHPSEHMMFPTYPGLPEDGITVTFDREMY",
    "response#2": "MRAGLIPHQLYIAHEVGRRHAPRVLLADEVGLGKTIEAGMIIHQQVLTGRAERILIVVPETLQHQWLVEMMRRFNLHFSIFDEERCVEAFSEADNPFETQQYVLCSLDFLRKSRQRFEQALEAEWDLLVVDEAHHLEWHPEKPSREYQVIEALAEQTPGVLLLTATPEQLGRESHFARLRLLDADRFYDYEAPRVLLADEVGLGKTIEAGMIIHQQIMDGRAERVLIIVPESLQHQWLVEMLRRFNLRFSLFDDSRYSESLLDSDNPFETEQMIICSLDFVRKNKQRFEHLVEATWDMLVVDEAHHLVWSEKAPSREYQVIETLAEAIPSVLLLTATPEQLGQESHFARLRLLDPNRFHDYNEFINEQQKYRPVADAVTILLSEDDLNNEQQNSISEMISEQDIEPLLKASNTQGEERTKSRQALIHMLMDRHGTGRLLFRNTRSGVKGFPNRLLHAIKMPLPTQYQTAIKVAEIMATGFKLWSLQKSWAKRVFLGRSFAIVAPTGVGKTTFGLVLSAFYDGKVLIVVPTRMLGKQCEKKLNDFLKNSQISRKVLFYESKASVKRKFEEGDFDILIGTNAFLQKNFEKIIKIDFRLIFIDDVDSFLKRSKNIDNLFHLLQFKEEEIKLALKPQKTEKDWEELDRIRKKERKKILLLSSATLVPKSSRISLFRNLLGFDIQKAVTTLRNVEDIRVFLKKHFPDNTYVAIWKDMSRKDGPKVSRLMQQSTQAKLINLKKTCILAAREAKRWQVKNTKNQKDLSTKARRAMREMFNFWKRNERIERELKKKHEKELVEKAKREEEERESKRQSRKLNFLITQTELYSHFIGKKIK",
    "response#3": "MRAGLIPHQLYIAHEVGRRHAPRVLLADEVGLGKTIEAGMIIHQQVLTGRAERILIVVPETLQHQWLVEMMRRFNLHFSIFDEERCVEAFSEADNPFETQQYVLCSLDFLRKSRQRFEQALEAEWDLLVVDEAHHLEWHPEKPSREYQVIEALAEQTPGVLLLTATPEQLGRESHFARLRLLDADRFYDYEAEVGQRFAPRVLLADEVGLGKTIEAGLIIHQQLLTGRAERILVIVPDTLRHQWLVEMLRRFNLRFSVFDEDRCVEAYADHDNPFYTEQLVICSLDLLRKKRRLDQALDADWDLMVVDEAHHLEWSEEAPSRAYQIVEALSEEIPGVLLLTATPDQLGHQSHFARLRLLDPDVGRRHAPRVLLADEVGLGKTIEAGMIIHQQLQAGRAERVLIVVPETLQHQWLVEMLRRFNLRFALFDDDRYAQAQLDSDNPFDTEQMIICSLDFVRRNKQRLEKLADAEWDLMVVDEAHHLVWSEDAPSREYQVIEQLAEQVPGILLLTATPEQLGMESHFARLRLLDPGSLERVDWLIDFLKNNRSKKVLIIASQAETALSLEEALRTREGIQATVFHEGMSIIERDKAGAYFAQESGGAQALICSEIGSEGRNFQFASNLVLFDLPLNPDLLEQRIGRLDRIGQKNDVEIHLPFLANTAQERLMQWYHQGLNAFECTCPSGHILFNEFSGELLESLLNNDEAVLESLLDGTKARYQELRVLLADEVGLGKTIEAGMIIHQQVLTGRAERILIVVPETLQHQWLVEMMRRFNLHFSIFDEERCVEAFSEADNPFETQQYVLCSLDFLRKSRQRFEQALEAEWDLLVVDEAHHLEWHPEKPSREYQVIEALAEQTPGVLLLTATPEQLGRESHFARLRLLDADRFYDYEAFVKEE"
  },
  {
    "reference": "MQPITQIPLTFDTVVNLLGSASGIGWILNYILMTYYSFRDKTYSMSMLPLCCNIAWEFVYGILCPSSTFVVRPVILSWLVLNCLVVYAAIKYSPNEWAHAPLVQRHLPLLFTVGIAACTGFHIALIRKFDPATAFLWSARSCQVLLSIGGLFQLLCRSSTKGGSYVLWLSRFLGSICGVLKMTLMWKYGESRFPWLDDPLTAYCIALWIISDVLYGVVFYSLRSKELAGAGKAKAI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be designed to localize specifically to membrane in order to perform its role in terpenoid biosynthetic process more effectively. The designed protein sequence is ",
    "response#1": "TYVLVQTLNFFYSSSMYIMSFVQLNLEKQCEYKNETIPISETCQIETESSKAFGNLNGEYCGIAENTLVNVTNQKASTNLLVDFDLSCSHWFFQEFGLTIFTIGAVIAVPFMSMLADRYGRKPIIVTTAILAFLANMAASFSPNFAIFLILRAFIGACSDSYLSVASVATCEYLSEKARAWITVVYNVAWSLGMVWTLLVTLMTDDWRWRYFIVSLPGVYGFALWYFLPESPHWLITKNKTEKLKKYIKTANRVNNVSPEFNDCQQSSHHEEKHESFKALLGSKKLIWLLFANGFIEMVISLVYFAISFMSVELGGDQVQAFLYSSLIEIPAGLAVIPLMMKMGRKMIVIWCLVFQTLALIGVTVFLDSYEFKLVIMLVAKVMATIIYSVHPIWATEQFPTSVRSLCFSLMNIPQSMGIIMSPYVKHIVMSPNWIPFVVIALFSFISATLAFMLHETKGKA",
    "response#2": "TYVLVQTLNFFYSSSMYIMSFVQLNLEKQCEYKNETIPISETCQIETESSKAFGNLNGEYCGIAENTLVNVTNQKASTNLLVDFDLSCSHWFFQEFGLTIFTIGAVIAVPFMSMLADRYGRKPIIVTTAILAFLANMAASFSPNFAIFLILRAFIGACSDSYLSVASVATCEYLSEKARAWITVVYNVAWSLGMVWTLLVTLMTDDWRWRYFIVSLPGVYGFALWYFLPESPHWLITKNKTEKLKKYIKTANRVNNVSPEFNDCQQSSHHEEKHESFKALLGSKKLIWLLFANGFIEMVISLVYFAISFMSVELGGDQVQAFLYSSLIEIPAGLAVIPLMMKMGRKMIVIWCLVFQTLALIGVTVFLDSYEFKLVIMLVAKVMATIIYSVHPIWATEQFPTSVRSLCFSLMNIPQSMGIIMSPYVKHIVMSPNWIPFVVIALFSFISATLAFMLHETKL",
    "response#3": "VRMGQGIDAHRFGPGDAVWLCGVEIRHDRGLVGHSDADCGLHALTDAVLGAIAEADIGEHFPPSDPRWKGASSDRFLAHAVGLVAARGGRLINADVTLVCERPKIRPHRDAMRARIAELLGLPVDRVSVKATTTEGMGFTGREEGLMAQAVVTVEA"
  },
  {
    "reference": "MKFIVKLFPEIIMKSETVRKRFVKVLTGNIKEILHRIDESVVVIRHWDFIEVRSQDESKRALIKDALSRISGIHHYLEVDETTYTDLHDIFLQTLAEYQDKLIDKTFCVRVKRRGKQDFDSMQVMQYVGGGLNQHIESAKVQLKNPDVTVRIEIENDKMMLVKARHEGLGGYPMSTQEDVLSLISGGFDSSVSSFSFMRRGSRVHYCFFNLGGQAHEVGVKQMAYHLWSRYSSSHKVKFVTVNFEPVVAEILRSIDDGQMGVVLKRMMVRAASQIAERMGIEAIVTGEALGQVASQTLTNLHLIDKASSRLVLRPLITHDKEHIVKITEQIGTFEIAKSMPEFCGVISKSPTVKAVESKIIATEADFDFAVLQTAIDTAVVSDIRALSQVDKAVEPQIVHQVSAGEVVIDVRSPDETDESPLQIDAPVIELPFYKIGSGFGQLDQTKTYLLYCHQGVMSRLQAGQLLANGYHNVKVLSLKKTADSDA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A key design requirement for the protein is the optimization of the Cysteine persulfide intermediate to achieve high catalytic efficiency for [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 +   S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier   protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-   cysteinyl-[cysteine desulfurase].2. The designed protein have THUMP domains with specific sequence motifs that are crucial for its activity.3. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.4. A protein that is capable of catalyzing reactions within the Cofactor biosynthesis pathway with high efficiency and specificity.5. A protein that contributes to tRNA thio-modification, thiazole biosynthetic process by functioning in cytoplasm with tRNA binding, tRNA U4 sulfurtransferase, ATP binding.6. The protein must exhibit the following characteristics: The sulfur is donated as persulfide by IscS.; This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The designed protein sequence is ",
    "response#1": "GSMEQVLTLMSGGFDSTVAAYQMLRRGLTTHFVFFNLGGRAHELGVKEVAHYLWQKYARGHKLLFVTVPFEGVVGEILSQVDNSQMGVILKRMMLRAATQIADELEIATLVTGEAIGQVSSQTLPNLGIIDRATDKLVLRPLIVSHKQDIIDTADAIGTGDFARNMPEYCGVISVNPTTRACPRRIAREEEKFTHIGSSDRETIVKTFQEITIKSRPVRKRFIRQLAKNIRAVLRDLDPELKVEGEWDNLEVETAVVDAKARREMIERLTCTPGIGHFLEVHEYPLGDFDDILAKCKAHFGDQLAGKTFAVRCKRAGKHAFTSMEVERYVGSGLRRECGAAGIDLKQPEVEVRMEIRLDRLFVIHRQHPGLGGYPLGALEQVLVLMSGGFDSTVAAYQMMRRGMISHFVFFNLGGRAHELGVMEVAHYLWEKYGRSQRVLFVSVPFEEVVGEILTKVDDSYMGVTLKRMMLRAASRVAERLELDALVTGEAISQVSSQTLPNLSVIDRVTDTLVLRPLIVSHKQDIIDTARQIGTAEFARHMPEYCGVISVNPTTQAKPYRVEHEESKFDIIKLFPEIMIKSDSVRKRFIKILTGNIRNILDKHDDSVAVVRHWDFIEVRSKHEENRPHLIELLQCIPGIHHFLEVEERPFTDLHNIFEQTLERMRDELVDKTFCVRAKRRGKHSFSSLEIERYVGGGLNQHIETAKVKLKNPDVTVRIEVDNDKLLFIQARHEGIGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMVYHLWNRYGSSHKVRFIAINFEAVVGEILEKIDNGQMGVVLKRMMVRAAGKVAQRFAIEAIVTGEALGQVSSQTLTNLRLIDEAAGTLVLRPLITHDKEQIIAMAKQIGTEDIAKSMPEFCGVISKNPTVKAVRERILAEEEHFD",
    "response#2": "MLLLEALASTPGVDQFLEVDRFPLPTMAGILEIADRYYGPSLPGKTFAVRCKRMGRQSFKSVDVEQAVGAGLNLKYGSARVKLKDPEVTVALEVREQELFVVKQPEQESFLSKILTWIVPLVIITVWFFFLRRMQAGGGAGGIFGVGKSRAKLFDEEKNKVTFDDVAGLEEAKEEVQEIVDFLKEPQKYTRLGGKCPVIGTQGDILSLMSGGFDSTVASYLTMKRGIKTHFIFFNLGGMAHEIGVKQVALYLWNKFGSSHKVKFVSVAFDDVLTEIFRATPPTYMGVMLKRLMLQAAEKVANDMEIDALVTGESVAQVSSQTLRNLALIDEVSNKLILRPLATMNKPDIMNIANEIGTRHFAESMPEYCGVISQNPITHGSHKRMEKVAQKFDLEDRLEIIKVHTKNKKMDSKHILQVEERAFTDLHNIYEQTFEMYAASLENKTFCVRAKRRGKHPFSSIELERYVGGGLNQHIPSAKVKLTHPDVTVNLEVEDDKLILVVREEDVFDKVLYGKERRSLEQTLVLMSGGFDSTVAAYQILRRGLMTHFCFFNLGGRAHELGVMEVAHFIWKKYGSSQRVLFVSVPFEEVLGEILGKVDDSHMGVVLKRMMLRAASSIAERLHIDALVTGEAISQVSSQTLPNLSVIDCVTDKLVLRPLIVAHKQDIIDTADQIGTGDFARHMPEYCGVISVNPKTAAKRGRVEHEEQEFELAVDAIISGETNPIPGAVVIDVRHPDETERRPLQISGTTVVDMPFYELHSKFAGLDRSCTYLLYCDKGVMSRLHAAHLVEQGYSNVKV",
    "response#3": "MLLLEALASTPGVDQFLEVDRFPLPTMAGILEIADRYYGPSLPGKTFAVRCKRMGRQSFKSVDVEQAVGAGLNLKYGSARVKLKDPEVTVALEVREQELFVVKQPAKLFPEITIKSRPVRRRLIQRLQTNLQILLQRVDERVKVSGHWDKVEISFPGQDIELSARLVDEMQRVPGIQSIVEVQEYPLEDFDQVFQLTRDAYAERLRGKTFKVRVRRSGDHDFTSVELERYVGGGLRQHTDAAGVDVRNPEVWVDLEVRNETLFVVTAIHQGLGGYPLGTQEDVLSLISGGFDSVVASYMTMRRGSKTHFLFFNLGGYAHEIGVKQVALYLWKRYGASARVKFITVPFEEVVGEILQSVHHSHMGVVLKRMMMRAADRVADRMELNAIVTGESVAQVSSQTLPNLRLIDESCEHLVLRPLVTMDKPDIIEISRQIGAFDFASAMPEYCGVISDKPTTHAKRDRIEREEAEFDKLFPEITIKSRPVRRRLIQRLQTNLQILLQRVDERVKVSGHWDKVEISFPGQDIELSARLVDEMQRVPGIQSIVEVQEYPLEDFDQVFQLTRDAYAERLRGKTFKVRVRRSGDHDFTSVELERYVGGGLRQHTDAAGVDVRNPEVWVDLEVRNETLFVVTAIHQGLGGYPLGTQEDVLSLISGGFDSVVASYMTMRRGSKTHFLFFNLGGYAHEIGVKQVALYLWKRYGASARVKFITVPFEEVVGEILQSVHHSHMGVVLKRMMMRAADRVADRMELNAIVTGESVAQVSSQTLPNLRLIDESCEHLVLRPLVTMDKPDIIEISRQIGAFDFASAMPEYCGVISDKPTTHAKRDRIEREEAEFD"
  },
  {
    "reference": "MAREXSKENTR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining translation elongation factor activity. The designed protein sequence is ",
    "response#1": "ELLKEEADEDLQAELEKDIKAYVATISEFELKLMLSDPYDKNNAILELHPGAGGTESQDWGSMLLRMYQRWSEKKGFKVEMLDYQAGDEAGIKSVTLLIKGHNAYGYLKAK",
    "response#2": "SVVTIKRIERNKRKFVTSVTGLEAFGLELKKVAKDFGKKFATGASVTKVPSGGEEIVVQGDVSGEIEEFLLEKYKDIPEDNIELVN",
    "response#3": "EVRIAELEDQMLDPNFWNDQQAAQKVINESNGYKETYQAFHALEEEQESMEISLELLKEEADEDLQAELEKDIKAYVATISEFELKLMLSDPYDKNNAILELHPGAGGTESQDWGSMLLRMYQRWSEKKGFKVEMLDYQAGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSR"
  },
  {
    "reference": "KEGYLMDHEGCKLSCFIRPSGYCGRECAIKKGSSGYCAWPACYCYGLPNWVKVWERATNRCGKK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Beta toxins bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing.2. The designed protein must possess toxin activity to support defense response under certain conditions. The designed protein sequence is ",
    "response#1": "KEGYPMGRDGCKISCVINNNFCKVECQAKWRQSDGYCYFWGLSCYCTIYQKTPRFGILAPINVEDNVTVTQCR",
    "response#2": "KEGYPMGRDGCKISCVINNNFCKVECQAKWRQSDGYCYFWGLSCYCTIYQKTPRFGILAPINVEDNVTVTQCR",
    "response#3": "KEGYPVGRDGCKISCVINNNFCKVECQAKWRQSDGYCYFWGLSCYCTNLPEDAQVWDSSTNKCR"
  },
  {
    "reference": "MELLIKNARIIDAIQDFKGDIYIKDGVINEIAQEIKKDNVEVLNCEEKILMPAFIDTHAHFRDPGLTCKEDLESGSKAALRGGYTGVCLMANTKPICSSKEVVQYVRDKSKELDLIDIHQCISVTQNFDGKTLDHLNEFKDDNEVKAISDDGVGVSNSNIMLEAMKIAKENNWVLMSHAESPEFSKSDMRIAENMMTIRDVELAKLSGAHVHMCHVSTKEALKCIIAAKDEGANITLEVTPHHIGLTKEINDYRVNPPIREKEDVEEIIKAIKMGNVDTIGTDHAPHTLEEKSKGSPGMVGLETAFPICYTKLVRENGVSLNELSKLMSLNPARLLGMNKGRISIGVEADLVLIDIDKKIKVDSNEFVSKGRNTPFEGMEYYGEVLATIKSGKIKYKK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should consider the endogenous proteins and enzymes present in the Pyrimidine metabolism, (S)-dihydroorotate from bicarbonate: step 3/3, UMP biosynthesis via de novo pathway pathway to avoid interference with native metabolic processes.2. The protein must exhibit zinc ion binding.3. The protein must exhibit the following characteristics: Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate..4. The designed protein should have a binding site that is specific to the target Zn(2+), substrate ligand.5. The protein with the reaction (S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate, the active site must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "DWHLHLRDGDMLKTVVPYTSEIYGRAIVMPNLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAAKLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIESVMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRPHLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTALGSYATVFEEMNALQHFEAFCSVNGPQFYGLDRHGDYSFMEDWHLHLRDGAMLRAVLPETTAHFARAIIMPNLVPPVVTFAQAQDYHARILAALPEGAEFTPLMTLYLTEDTDPDDVAAAHANGLVHAVKLYPAGATTNSASGVANFDNIRAVLERMAEIGLPLCVHGEVTDDDIDIFDREAVFIDRVLDPIRQATPGLRVVMEHITTRNAVDYATAQDDTLGATITTHHLVINRNHILAGGIKPHYYCLPVAKREEHRLALRAAATSGDARFFLGTDSAPHTDANKLLPCGCAGCFTATNTMSILAHVFEEEQALERLEGFASLHGPAFYRHGDAWAGYADSVDWHIHLRDGAALPHTVADAARQFARAIIMPNLVPPVRNADEAEAYRQRILAARPAGSRFEPLMVLYLTDSTTPDDIRRAKASGFVHAAKLYPAGATTNSASGVTAIDNIFGVLETMAEVGLPLLVHGEVTRSEIDIFDREKYFIDEQLSRVTARFPTLKVVFEHITTRDAVQFVQAAGANVGATITAHHLLYNRNHMLVGGIRPHLFCLPVLKRNLHQEALLDAATSGSPKFFLGTDSAPHAQHAKEAACGCAGCYTAFAAIELYAEAFEQRQALDKLEPFASHFGPDFYGLPRN",
    "response#2": "DWHLHLRDGDALADVVGDTARQFGRAIIMPNLKPPVTTTAQARAYRERILAALPAGTRFEPLMTLYLTDNTTPEEVRAARASGFVHGVKLYPAGATTNSDAGVTDLRRCAKTLEAMQDVGMPLLVHGEVTDPTVDIFDREAVFIDTVMQPLRRDFPALKVVFEHITTKHAAEYVRDAQGPVGATITAHHLLYNRNALFVGGIRPHYYCLPVLKRETHRLALVAAATSGHPRFFLGTDSAPHAKGLKEHACGCAGCYTALHAMELYAEAFEDANALDKLEGFASLHGPDFYGLPRNAGTLS",
    "response#3": "DWHLHFRDGAMMRGVVPYTARQFARAIVMPNLTPPVTTTALGAAYRERILAAVPEGVDFRPLMTAYLTDETDADDLIDGYTHGVFTAAKLYPANATTNSASGVTDVEALYPVFERMAAAGMVLCIHGEVTDADVDVFDREKEFIERTMAPLHAAIPALKIAFEHITTSDAVDFVVGANDNIGATITPQHLHINRNAMLVGGIQPHNYCLPVAKRETHRLALRQAATSGPPKFFLGTDSAPHEKHTKESACGCAGIFGAPYALESYLAVFEEEDALDKFEAFASLNGPAFYGLP"
  },
  {
    "reference": "MTSSEVELPGEVKNDPAALMASLHLMPSPVPNPEIKYTKIFINNEWHDSVSGKVFPTYNPATGEKICDVQEADKADVDKAVQAARSAFSLGSVWRKMDASERGKLLFKLADLVERDSAYLATLESLDSGKPFLPCFFVDLQGIIKTFRYYAGWADKIHGSTIPIDGEFFTLTRHEPIGVCGQIIPWNFPLVMTAWKLGPALSCGNTVVLKPAEQTPLTCLYLGALIKEAGFPPGVVNILPGYGPTAGAAISSHMGIDKVAFTGSTEVGKLIQEAAGKSNLKRVTLELGGKSPNIIFADADFELALEQAHQGVFFNNGQCCTAGSRIFVEEPIYDEFVRRSVERAQRRKVGNPFDPTTEHGPQVSEEQQRRVLELIQSGITEGAKLECGGKAPATKGFFVEPTVFSNVKDHMRIAKEEIFGPVQQIMKFKTIEEVIERANNTEYGLAAAVFTRDISKAMTISAAVQAGTVWINCYNAMSCQCPFGGFKMSGNGRELGEIGLKEYTELKTITMKMSGKTS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design must be focused on enhancing the efficiency of hindbrain morphogenesis, embryonic viscerocranium morphogenesis. The designed protein sequence is ",
    "response#1": "PLFTNEEHSDMHAYIAMFGTVTLLCTFFTLATFLADWKNSNRYPAVILFYVNACFFMGSIGWLAQFMDGARKEIVCKSDNTMRLGEPSSTETLSCVIIFSLVYYSLMSGVIWFVMLTYAWHTSFKALGTTHQPLSGKTSYFHMVTWSIPFILTVSILAIAEVDGDSVSGICFVGYKNYRYRAGFVLAPIGVVLVVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGIFGFLAFGFVFITFGCHFYDFFNQAEWEKSFREYVLCEANVTIAHQTNKPIPECTIKNRPSLLVEKINLFSMFGTGIAMSTWVWTKATILIWKRTWGGCFPGDAQVMLEDGTTKLMHDLLPRDRVLASSASDGRGPLLYSPVLSFLDRQPNVTKIFYIIGTNSGLNITLTAAHLIFVTDCTREPNGKETAEGPLVGSVRETRPSWESGLRTVFASDILPGQCVLTSV",
    "response#2": "HEDMKDALLKKLGLNEIPQIHKRDLENLVIPTHVKNKYISMLKLHHSRKRRSLPSLAGILRGIPGNADISGEFVYSDTTRQRVVFEMTSRIPENSEVTMAELKLYKKAPHKRSIPERKGHRPVNNARVSIYWVEPQKDGSNRTSLVDSRLIPIHETGWKSFDVTQAVQYWSRSRMEMPMHLV",
    "response#3": "LFTNEEHSDMHAYIAMFGTVTLLCTFFTLATFLADWKNSNRYPAVILFYVNACFFMGSIGWLAQFMDGARKEIVCKSDNTMRLGEPSSTETLSCVIIFSLVYYSLMSGVIWFVMLTYAWHTSFKALGTTHQPLSGKTSYFHMVTWSIPFILTVSILAIAEVDGDSVSGICFVGYKNYRYRAGFVLAPIGVVLVVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGIFGFLAFGFVFITFGCHFYDFFNQAEWEKSFREYVLCEANVTIAHQTNKPIPECTIKNRPSLLVEKINLFSMFGTGIAMSTWVWTKATILIWKRTWCGGCFPGDAQVMLEDGTTKLMHDLLPRDRVLASSASDGRGPLLYSPVLSFLDRQPNVTKIFYIIGTNSGLNITLTAAHLIFVTDCTREPNGKETAEGPLVGSVRETRPSWESGLRTVFASDILPGQCVLTSP"
  },
  {
    "reference": "MKNIRNFSIIAHIDHGKSTLADRLIQECGAVSSREMSSQIMDTMDIEKERGITIKAQSVRLNYTLDGQNFVLNLIDTPGHVDFSYEVSRSLASCEGALLVVDASQGVEAQTIANVYIALENNLEIIPVINKIDLPAADPQRVKDEIEHVIGLDCSGAIEVSAKTGAGINELLEAIVRRVPAPDANDALPTKALIYDSWFDNYLGALALVRIYDGEIKKNDEVLVMGTNKKHIVLDLMYPHPIAPIKTARIGTGEVGIIVLGLKNVSDVQVGDTITLAKNPTKEPVGGFERAKPFVFAGIYPIETDKFEDLRDALDKLKLNDSSISYEPETSIALGFGFRVGFLGLLHMEVIKERLEREFDLDLIATAPTVTYEVVQTDGQILRIQNPSQLPAVNKIEMIKEPYVRSTIITPTEFLGNIITLLNNRRGVQTKMDYITPERVLLEYDIPMNEIVMDFYDKLKSSTKGYASFDYEPSDYREGDLVKLDIKVAGETVDALSIIVPESKAQSKGRDFVKAMKEIVPRQLFEVAIQASIGNKIIARETVKSMGKNVTAKCYGGDITRKRKLLEKQKEGKKRMKAIGKVNLPQEAFLSVLKID",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have translation elongation factor activity, GTPase activity, ribosome binding, GTP binding and be active in plasma membrane.2. The protein contains novel tr-type G domains that confer a unique function or activity.3. For general function, the protein need meet that Required for accurate and efficient protein synthesis under certain stress conditions; Binds to ribosomes in a GTP- dependent manner.; Back-translocation proceeds from a post-translocation (POST) complex to a pre- translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly The designed protein sequence is ",
    "response#1": "IRNFSIIAHIDHGKSTLSDRIIQICGGLTERELKEQVLDSMDLERERGITIKAQSVTLDYKAEDGQVYQLNFIDTPGHVDFSYEVSRSLYACEGALLVVDAGQGVEAQSVANCYTAVEQGLEVLPVLNKMDLPQADPDRVAHEVEEIIGIDASDACRVSAKSGLGMEALLERLVRDVDNFSIIAHIDHGKSTLSDRIIQICGGLTERELKEQVLDSMDLERERGITIKAQSVTLDYKAEDGQVYQLNFIDTPGHVDFSYEVSRSLYACEGALLVVDAGQGVEAQSVANCYTAVEQGLEVLPVLNKMDLPQADPDRVAHEVEEIIGIDASDACRVSAKSGLGMEAN",
    "response#2": "IRNFSIIAHIDHGKSTLSDRIIQICGGLTERELKEQVLDSMDLERERGITIKAQSVTLDYKAEDGQVYQLNFIDTPGHVDFSYEVSRSLYACEGALLVVDAGQGVEAQSVANCYTAVEQGLEVLPVLNKMDLPQADPDRVAHEVEEIIGIDASDACRVSAKSGLGMEALLERLVRDVS",
    "response#3": "NFSIIAHIDHGKSTLSDRIIQICGGLTERELKEQVLDSMDLERERGITIKAQSVTLDYKAEDGQVYQLNFIDTPGHVDFSYEVSRSLYACEGALLVVDAGQGVEAQSVANCYTAVEQGLEVLPVLNKMDLPQADPDRVAHEVEEIIGIDASDACRVSAKSGLGMEAR"
  },
  {
    "reference": "MQEFDKSISFDGRDIRLKMGTLAPQAGGSVLIQSGDTAVLVTATRAKGRDGIDFLPLTVDYEGRLYAAGRIPGGFLRREGRPPEKATLISRLIDRPLRPLFPHWLRDELQIVATTLSMDEEVPPDVLAVTGASVAVILAQIPFKGPMAAVRVGLVGDDFIINPTYREVHNGDLDLVVAGTPAGIVMVEAGANQLPEQDIIEAIDFGYEAVQDLINAQRELMTDLGITLATSEPPPVNTAVEEFIANRASKKIITVLGQFDLGKDGRDAALDEIKATEVETAIAELPETDPVKQSVEEDPKLVGNLYKALTKKLMRKQIVDDGVRVDGRKLEQVRPISCEVGFLPRRVHGSGLFNRGLTQVLSLATLGSPGDAQDLADDLHPEDEKRYLHHYNFPPYSVGEARPMRSPGRREIGHGALAERAIIPVLPPQEDFPYVVRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPIKKPVSGAAMGLIKEGDEIRILTDIQGIEDFLGDMDFKVAGTDSGITALQMDMKIDGLSMEVVSKAIMQALPARLHILDKMLATIREPRPELSPFAPRLLTLKIEPEHIGMVIGPGGKTIKGITEQTSCKIDIADDGTVTIASSEGERAERARQMIYNMTRKLNEGEVYLGRVTRIIPIGAFVEVLPGKEGMIHISQLTEGRVGKVEDEVGVGDEVIVKVREIDSKGRLNLTRLGIHPDEAAEARRNASRG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must bind to Mg(2+) in order to perform its enzymatic function.2. The designed protein should have a specific function within the cytosol. The designed protein sequence is ",
    "response#1": "VGIAAGSQTYPGAAVLSVGSALAAGAGMVRFAGAPHAAEQVRSRWPEAVVTEHEGSDVPEAGRVQAWVVGPGLGTDERAAATVEALLGTDVPVLVDADALTVCAAHPDWLRRRSAPTLLTPHDREFARFGTPVTADRIGSARRLAAELGVTVLLKGDATVVTDPTGPARVNGTGSPALATAGSGDVLSGGCGALLAQGLSAIDAGSTGAHLHGRAGALAARL",
    "response#2": "VGIAAGSQTYPGAAVLSVGSALAAGAGMVRFAGAPHAAEQVRSRWPEAVVTEHEGSDVPEAGRVQAWVVGPGLGTDERAAATVEALLGTDVPVLVDADALTVCAAHPDWLRRRSAPTLLTPHDREFARFGTPVTADRIGSARRLAAELGVTVLLKGDATVVTDPTGPARVNGTGSPALATAGSGDVLSGGCGALLAQGLSAIDAGSTGAHLHGRAGALAARR",
    "response#3": "VGIAAGSQTYPGAAVLSVGSALAAGAGMVRFAGAPHAAEQVRSRWPEAVVTEHEGSDVPEAGRVQAWVVGPGLGTDERAAATVEALLGTDVPVLVDADALTVCAAHPDWLRRRSAPTLLTPHDREFARFGTPVTADRIGSARRLAAELGVTVLLKGDATVVTDPTGPARVNGTGSPALATAGSGDVLSGGCGALLAQGLSAIDAGSTGAHLHGRAGALAARVF"
  },
  {
    "reference": "MSSPLLGQSLSQLTQWVEQQGQPAYRGQQIYQWIYQRGARSLGEITVLPQQWRSTLDSVAIGRSSLHHRSQATDQTVKYLLRLQDGQIIETVGIPTAKRLTVCVSSQVGCPMGCLFCATGQGGLQRNLECHEIVDQVLTVQEDFGQRVSNVVFMGMGEPLLNRDAVLEAIRVLNQEVGIGQRSITLSTVGIPGQIRRLGETGQVGDRPLQFTLAVSLHASNQSVRHSLIPSAQAYPLEALLEECRDYVRLTHRRVTFEYILLARVNDRPEHAQELAQCLRGFQSHVNLIPYNPISEADYQRPDRHRIEAFSQILQKHRIAVSVRYSRGLDADAACGQLRATVAGAGA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein contains novel Radical SAM core domains that confer a unique function or activity.2. The Proton acceptor of the protein must be engineered to enable effective catalysis of adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.3. The presence of [4Fe-4S] cluster is crucial for the protein to function optimally under specific conditions.4. The designed protein should have a high specificity for S-adenosyl-L-methionine binding, with minimal off-target effects.5. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs..6. The protein must have high specificity towards 4 iron, 4 sulfur cluster binding, metal ion binding. The designed protein sequence is ",
    "response#1": "MDLEALQRTLDELGQPRFRAGQVWAWAARGAQGYGEMTNVPAGLREQLATEFGIGAVAEGSRTTICVSSQVGCAMNCRFCLTGRGGFVRDLKAHEIVDQVIAVQRLCAPRAVTNIVFMGTGEPLKNLSNVIEAVRRMNGLLKISRRRITVSTSGVVPEIDRLAGMSPSVNLAVSLNAATDELRSLLMPVNRRYPIRSLISACRRYPLEPRRRITFEYILFDGLNDSKRDAQRLVGLLRGIPSKLNLIPFNPYDGAEFRRPADGKVEAFRDMIQSRGITAVVRKSRGRDVAAGCGQLAAA",
    "response#2": "MVEGLGEPSYRGGQILEWLWQKRATSIEGMSNLPVRLREHLEESFTLRSLSPAKSQGSRDSTRKFLFRLNDGRYIECVLIPASPALYGERSDRRTLCVSSQVGCAFGCKFCASGLDGFTRNLTADEIAGQVLLAEELSGEKVNNIVFMGMGEPLANLPQLLKALELITSPWGLGIGARHLTVSTSGLVPQIRKLADHPQQIRLAISLHGASNDVRDRIMPVNRKWPVEDLFKALSYWTKRKNQKITLEYILIDGINADPAQARLLAQHAESLRAKVNLIPYNTVDGLKWKRPPEEECHAFRDILAARGIPATLRLEKGHDIDAACGQLRLKEERAEKLA",
    "response#3": "LSVCVTTQVGCNIGCSFCASGLLKKSRDLTSGEIVGQIMKAQLFLDERGEGERVSHIVVMGIGEPFDNYEHMSNFIRVVKDHKGLAIGPRHITVSTSGLADKIKEFADSDLGTNLAISLHAPNNELRTRIMIINRAIPLEKLMPAIDYYLERTNRRITLEYILLKDINDRVEHAQELADLIGEGKRKQLSNVNLIPYNPVDEHSQYKRTEQESVRAFYRRSK"
  },
  {
    "reference": "MMTHDVGVRELGLIEYRAAWQAMQHFTNTRDADSGDEIWLLQHPPVFTQGQAGKAEHLLFPGEIPVVQVDRGGQVTYHGPGQLVGYLLLDVRRLGIGVRELVSRIERSLIDLLAGYDVEAVAKPDAPGVYVGGAKIASLGLRIRNGRSFHGLALNVDMDLEPFRRINPCGYAGLPMTQLRDLIGPIDISEVADRLRDHLVRQLGYAQQKTLAGGIEAYE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should take into account the physiological conditions of the protein lipoylation via endogenous pathway pathway. The designed protein sequence is ",
    "response#1": "GNAVATILATLGTDVADPVQRLAAVTASTRAAKAQLRSMDKDAILAYSAALMAPYGVQLASTLSGVKPPWPYTFNLCVSNVPGPEDVLYLRGSRMEASYPVSLVAHSQALNVTLQSYAGTLNFGFIGCRDTLPHLQRLAVYTGEALDQC",
    "response#2": "LGPQDMFFLYSESSTTMMHVGALMPFTPPSGAPPDLLRQLVDESKASEVVEPWSLRLSHPELLYHPTQSWVVDDNFDLDYHVRRSALASPGDERELGIPVSRLHSHALDLRRPPWEVHFIEGLEGGRFAIYIKMHHSLIDGYTGQKMLARSLSTDPHDTTHPLFFNIPTPGRSPADTQDSVGGGLIAGAGNVLDGLGDVVRGLGGLVSGVGSVLGSVAGAGRSTFELTKALVNAQLRSDHEYRNLVGSVQAPHCILNTRISRNRRFATQQYPLDRLKAIGAQYDATINDVL",
    "response#3": "GNAVATILATLGTDVADPVQRLAAVTASTRAAKAQLRSMDKDAILAYSAALMAPYGVQLASTLSGVKPPWPYTFNLCVSNVPGPEDVLYLRGSRMEASYPVSLVAHSQALNVTLQSYAGTLNFGFIGCRDTLPHLQRLAVYTGEALDQC"
  },
  {
    "reference": "MAADGDGDQVPTEVFVAGFIDVKQYPAPDKDLIINYDSLTAALPLNQPIPLNVEHLEHAEVGAVVGIFKVVHGLFCVGKIYCKPFLDLVHRMFTSSSAAKKPSTLPRLPAVEMLHAWLPELSLSSTHPELLSATPPSQILQHIALCAMGKRRGVVAVYGLDPKWVISKFNSLSTDEREQVASGSMTGLDLIKNDPPCLDFTINTDTLLSKAIDAGFIQNRLDILQTDKAVVAVSPSTYLKASAGPDPGAVDKTEDTLLEATMNPHQPQSTEDSGLISVPKSALVSLLTKHDHAQAPNAPKMPAPAFQGTGFQVPAPAHAPYYPYQHQQFAYPGQMQPPAMGIYSPWQMPCIPWPMPPQDPERMPKRKRSFDEDAFPGENSKKGLYGELLTMANSLAALKQEVDALRGAASGMQHMLKQPWHTIASPFPSPAPMAHPPIPVQQPLYAGHSHPSLVAHSQLSSTSPQTAQPPAAAAQPVQVTGPAPADPMTHAGAAAPRSAPETSQPNPAPPAGGVPATGKIDASNAPNCPNLLQKMFCDELTK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein; Cleavages products are evicted from the capsid before or during DNA packaging.; Capsid scaffolding protein: Acts as a scaffold protein by binding major capsid protein in the cytoplasm, inducing the nuclear localization of both proteins2. A protein that localizes to host cell nucleus and can perform serine-type endopeptidase activity, identical protein binding with high specificity and efficiency.3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "MGXKFGXMXKXFX",
    "response#2": "LFPL",
    "response#3": "MXKVEXPEXXH"
  },
  {
    "reference": "MGSMGGSRHLKAINAPRYWPILRKEYRWVVKSSPGPHPISRSLPLLILVRDMLGYAKTGKEARRLIAEGYFKIDGRVRRDYKFPVGVMDVIEIVGVNKYYRVIPVPTKVLGLIEISREEASFKLCRIENKTTVKNGHIQLNLHDGRNVLIRVNDPRNPVEDTYDTLGVLKLSIPVQQILDYVPLKEGVIAIVSGGRNVGRVGKIVSIHKGMRRYRSIVTLEDKQGNKFQTSLDYIFPIGVEKPLIKLPEGAW",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein can be targeted to ribonucleoprotein complex, ribosome and perform structural constituent of ribosome, rRNA binding.2. The designed protein have S4 RNA-binding domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "TASELAKSFTEIDVLPIYAQAERGVLFGAVSAKQVVAELSKKGIEITTKNVVLGAPIKALGEHEVRIFLHSRVECSLKIHC",
    "response#2": "TASELAKSFTEIDVLPIYAQAERGVLFGAVSAKQVVAELSKKGIEITTKNVVLGAPIKALGEHEVRIFLHSRVECSLKIHR",
    "response#3": "TASELAKSFTEIDVLPIYAQAERGVLFGAVSAKQVVAELSKKGIEITTKNVVLGAPIKALGEHEVRIFLHSRVECSLKIHK"
  },
  {
    "reference": "MNVSNAPRFNLLTILGPTASGKTRLAVNLARELGGEIISADSRQVFRRMDIGTGKDLHEYGEVHHHLIDILEPGEEFSVFAFQRLFLEAVGDICGRGRLPLLCGGTGMYLDAALRRYRMHEVPEDREWRASLEGVGDGELASRLREFRPGLHNSTDLVDRQRTIRALEIARFQADCAGDDEPFPDLRPLVIGIRWERAELRRRITERLRQRLESGMIEEVRRLNDGGVPWERLDYYGLEYRFVGMYLRDELSRNDLFQKLNSAIHDFAKRQETWFRRMERNGVAINWVDGGGGPLSEARRVILDNSYHLATGR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have a binding site that is specific to the target substrate, ATP ligand.2. The desired protein should be able to perform adenosine(37) in tRNA + dimethylallyl diphosphate =   diphosphate + N(6)-dimethylallyladenosine(37) in tRNA3. The designed protein should be optimized for tRNA dimethylallyltransferase activity activity in order to improve tRNA processing.4. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "MPTYTVPARLVTP",
    "response#2": "MPKVYDAATTPARR",
    "response#3": "MPIGVPKVPFRSPGENPAHWLDLYRE"
  },
  {
    "reference": "MITKTQAKQTTISKETSLKGVGLHTGKDVEIKFLPAIANSGYVFIRKDLEGAPMIKADANLVTSTQRGTCLEKNGVSIQTCEHVLAALVGLEIDNVTIELNNAEPPIMDGSSKFFVEALESVGIKTLDVFRDVFVVKDVISYVDEETGSDITIIPSDEYQVTTMVDFGTKVLGTQNATLSKLSNFKSQISNSRTFSFLHELEMLLEEGLIKGGDLNNAIVYVDKPLSNETMGKLKEAFNKDSISVQPNGILDNLTLHYPNEAARHKLLDVIGDLALIGIRLQGKVIANKPGHFVNTQFAKKMAKIIKIERRNNMPAVDLNQTPVMDVNAIMEMLPHRPPFLMLDKVFELSPNHVIGSKNVTMNESFFQGHFPGVPVMPGVLIVEAMAQTGGILVLNTVPDPENYLTFFMKIDKVKFKQKVVPGDTLIFSCSLITPIRRGICHMQGFTYANGKLCAEAELMAQISKIK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding.2. A protein with 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity, (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity is needed to enhance lipid A biosynthetic process in a specific context.3. The active site of the protein must be engineered to enable effective catalysis of a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine +   H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate.4. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "MDRQIQFTTIEDAIEDIRAGRMVIVVDDADRENEGDLVIAAEKVTPESINFMATHGRGLICAPMSGARLDELEIPLMVTDNTSRMGTAFTVSIDHKDTTTGISAHERAHTIQALIDKKTKPSDFRRPGHIFPLIARDGGVLVRAGQTEAAVDLARLAGFYPAGVICEIMNDDGTMSRVPDLIEFSKKHSMKIISIKDLIAYRLKNESLVKREV",
    "response#2": "MSAAADQPAGGDRPSRAPVVLLLRGNPGTGKTTVARLLAERTGALLVESDEIRSRMGGLLPADPQDPAVAELVFRVARRHLAAGTPVVIPTMVDPVTARLARRLADAAGLPLTVVELTATVEECERRDRARRAAGGAVGGVPGPVSPPSTVVRTGFGTWPPGVADPEVVLAPSTADVAGRVAAVLGG",
    "response#3": "MSNSLTDALQSIRDGKLVIVVDDESRENEGDLICAAQFATPEAINFMATHGRGLICAPVTEKLADSLNLHMMVGSNTAANETAFTVSVDLLGHGTTTGISAQDRAKTIQALINPATTVADLSRPGHIFPLRARAGGVLARTGHTEAVVDLARLAGLYPAGVICEIQNDDGTMARLPDLEKFSKEHNLSIISIRDLIEYRLRNESLIERVESATSAEMPEHLDGESEPCDQTDTSPIEMTEVDTPDTNTDSTDSAIDTTASESETAADVESSPLLQFGEAIPSS"
  },
  {
    "reference": "MKIPRGIKIILRWVVTLYIYGFILYQITPFSYFETYIPEERLAPKPYVERIFYLFGVKSNEYPSKEVLEFLKSMDVDLVYGFFPFEDYGIFKNSLKAPECHLIYTSEISTFHRILNFLFDYVPKKITGNETRTFHYFIKPKLGKCNVIVQDVYLSPNFLDFKLDHSRNMVYKRLWEDLQLEQNVITNGRKSVDVYAYSTKSMYYPSESTIYPFKLYAKSNEEKTLIIVYRDGKVYRIYDERSTVLKINKPGKYSVKILTYAFSWEGYYFGLRTIAYSAPITLLY",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should contain a Helical membrane-spanning region.2. The designed protein should have a specific function within the membrane. The designed protein sequence is ",
    "response#1": "IPAEVVLPLAGYWVSKGDMTLAGVVLAGSLGGVAGPLTLYWIGRYGGRPFLERFGKYLFIKPEALDKSDNFFKKHGGFVAFSGRFLPGIRTLISIPCGIAKMNVWVFSLYTFIAMLPITFVYVYLGN",
    "response#2": "IPAEVVLPLAGYWVSKGDMTLAGVVLAGSLGGVAGPLTLYWIGRYGGRPFLERFGKYLFIKPEALDKSDNFFKKHGGFVAFSGRFLPGIRTLISIPCGIAKMNVWVFSLYTFIAMLPITFVYVYLGS",
    "response#3": "IPAEVVLPLAGYWVSKGDMTLAGVVLAGSLGGVAGPLTLYWIGRYGGRPFLERFGKYLFIKPEALDKSDNFFKKHGGFVAFSGRFLPGIRTLISIPCGIAKMNVWVFSLYTFIAMLPITFVYVYLGS"
  },
  {
    "reference": "MRNRQNQKNWTNLRGLLSVEEREKLENLRLELVCMQAANSIPHDPPEISSLETELICLTTNTKDCKPVRFHSDLLLKKHKYNEIKKIFKEILENIEAYRDEFTKNQTKINLLLADDARASLRNRSLDFSDLMPSSIIKDVQVLANMEANVVVMKNALKTKLVGEKSVPVASSPISSIIPRTSRNKKTSPSNYHHSVLSHRKSNEWNQVSSTEYKRTLLLNIKYNDDFKATIVPSFESCLCSRSYFLRVKLHFDKGVGSAEIDIPVQVKNSFI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be able to modulate protein polyubiquitination in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "PVLLSDIREKRYENLMVVSPDVGGVLRARAMAKQLGVDMAIIDKRRPKAGVAEVMHIIGDVKDRTCVIIDDMIDTANTLCKAAQALKQHGAKRVLAYATHAVFSGAAVERIMQSDLDEVVVTDKRALVILAKGAEEMETVIPVDVMRRAGVCSALYIELWRLVLMQGCCVHHVCRRLGPYNVVLLPGGLLGAQNLSESPAVKEVLKDQEGRKGLIAAICAGPTALLAHGIAYGSTVTTHPGAKDKMMAGDHYKYSEARVQKDGNVITSRGPGTSFEFALTIVES",
    "response#2": "PVLLSDIREKRYENLMVVSPDVGGVLRARAMAKQLGVDMAIIDKRRPKAGVAEVMHIIGDVKDRTCVIIDDMIDTANTLCKAAQALKQHGAKRVLAYATHAVFSGAAVERIMQSDLDEVVVTDA",
    "response#3": "PVLLSDIREKRYENLMVVSPDVGGVLRARAMAKQLGVDMAIIDKRRPKAGVAEVMHIIGDVKDRTCVIIDDMIDTANTLCKAAQALKQHGAKRVLAYATHAVFSGAAVERIMQSDLDEVVVTDG"
  },
  {
    "reference": "MDGCRIAVIGATGAVGSVFLSILEERSFPATEVRLCASERSWGRKLTALGEERTVEEATPELLGEVDLVFVCAGSGISREIGPLAVDNGASVVDKSSAFRMDPDVPLVIPEINGDDLESHRGIVSVPNCSTAPLAMTLKPLMGVNSIERVVVSTYQSVSGTGAAAVEELRNQSASSLDNGPIEPSEYPHQIAFNVLPHVETFEDNGYTTEEMKMVNETRKILHEPNLLISATCVRVPVMVGHSEAVHIDFEEPIGPGEVREALSDVPGLKVVDDPAMNRYPMPVDAEGEDDVFVGRIRQDVSNPRGVAMWLVTDNLRKGAALNGIQIAEEMMSRGLLNTPARRG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should interact with enzymes and substrates within the Amino-acid biosynthesis, L-threonine biosynthesis, L-threonine from L-aspartate: step 2/5 pathway to enhance or regulate its activity.2. The designed protein should have a binding site that is specific to the target substrate, phosphate ligand.3. A protein with NAD binding, protein dimerization activity, aspartate-semialdehyde dehydrogenase activity, NADP binding is needed to enhance lysine biosynthetic process via diaminopimelate, diaminopimelate biosynthetic process, threonine biosynthetic process, 'de novo' L-methionine biosynthetic process, isoleucine biosynthetic process in a specific context.4. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..5. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "MTNVAVVGACGMVGGEMIRTLERRNFPVKSLRPLDVAANEGTKVVFRGEDVEVLEAKKDNFADIDVAIFSAGAGASLKLAPEAVEMGAVVVDNSSAWRMNDQCPLVVPEVNPEDLRKIVGVVGATGAVGRKMLEVLEERNIDIEKLRVFASARSAGEKINFKDQVVTVELLTEEVMKDKFDYLLFSAGGSVSAQYAPIAAEAGNTVIDNSSQWRMTEGIPLVVPEVNAHVLKGARKGIIANPNCTTMAAMPVLAPLAAKRTIRRVVVSTYQSASGAGARAMEELKQLSKVVLDGGTPVSEVLPHPLAFNLFLHDSPLQENSYCXEEMKMVNETRKIMGLKDLPFTATCVRVPVLRAHSEAVNIAFDQPFPVEEARSLLKXAPGVELIEDVASNRFPMPTDVTGRDPVAIGRIRQDISDPNALELWLXGDQIRKGAALNAVQIAELVAAGG",
    "response#2": "MTNVAVIGATGAVGQVALQILEERAFPMNSLRLCASPSSVGKKITFRGVEIEVETPTESLFKDTDIAFIAAGSSLSKIYAPLAASLDCIAIDKSSAFRMDPKVPLVVPEINANDMSLVAGTCVRVPVFTGHLAPLHKRYGIDKVVVSTYQAVSGSGHKAIVELENQMKDANYPNNVYPKRIATNCIPHIDVFNDNGFTKEELKMVYETQKILEDNKIKVNSTTVRIPVFFSHSESVYVEFKETPEVSEVKQILSEAPGVIVLDNPEKNEYPTPLEVANSDDTYVGRIRKDLFNPNALSMWVVADNLRKGAALNTLSPLHDVNPIRRVVVDTYQSVSGTGKAAMEELQVQSSQILEGLPIKPEAYPHQIAFNALPQVETFLDDGYTTEERKMRDETRKILHSDSINISATCVRIPVPISHSEALHIEFEKPMSPSEARSLLSSFSGATVLDDPFDSKYPLASSATGRDDVFVGRIRADDSHPNGLVMWVVSDNLRLNPIHKLSRIKRVVASTYQAVSGGGALAMAECIEQAKQYLAGEKVRVERFAHQIAFNLIPHIDVFQENGYTKEEMKMVLETRKILHDPEMRIAATTVRVPVLRSHSESLNIETERPLSVEEIRTALAKAPGVIVEDDPANKIYPMPLFRAERDDVSVGRIRKDLSCENGINLWVSGDQILG",
    "response#3": "MSVVAILGATGVVGTQMLQCLEERDFPVSRLVPLASARSAGKTLTFRGEEVAIQEARPEAFEGVDIVLGAAGDDIAKALLPEAAARGAVCVDNSHAFRLDPDVPLVVPEINAGDIANAAERGIIANPNCTTMAAMPVLKPLHDLLSIERVVVSTYQSVSGAGKDPMNELAKQTKQFLNKEKIISENFTKQIAFNVIPHIDEFTDEGYTKEELKMVNETQKILSDKIKLTATCVRVPVLVSHCESVNIQFERSFTLDQIYSTLSSAKGCKVIDERKNGGYITPIEAEGQFDTYISRIRIDSTQKNCLNMWVVSDNLLKGAALNAVQIAELL"
  },
  {
    "reference": "MSSASRLQNVNIVSNNYSRYGTSVYDKLYHSNGSGSNNAGKNSTTVGKLSSISQKSRSKQRHGSNCSRSMSQSPLSTFKSPLSNQNQSSAPDDLASIGQRRSDDVTSLDNETIITMNSRKSRIKKKYKSLISTSSKKFMNKLYDHGASSDSFSIFSLKTSHSGKHENSRFEKLRKRKYHAWGKFADINDLPVEIIAKILSEFELGRDQKTLVRCLYVSKKFYKATKIVLYRLPYFTSTYRVAQFVTSLRLHPDNGAYVKVLDLSHLKPGIIGQDSKDSQGLDDDGHSRRHRRRRRRSTNTSLNLPPATPTSTISNEDDANSGLIKDDASNGSEVEDLALAGWRDWRYRNEPLYSSPLLNSFKLKKVVSRSSSITSTSSGNSTGVHSTRRQRSNSSVASITTSIMSSIYNTSHVSLSSTTSNTSNGNISSGSNLSRVSTAGSLKKASAKSTRSSPQKAKPISDITSSSWFRMRLSSRNRKARTANTINLKNSKDKSDDDFKVLKHDSGHPSNYRSSTLKFSIEQPFSTHHPYANKFLLKYAPYKDLPLGYILHMLNLCPNLVELNLSNLVICTDFKLINQRSERRRMTSSLLPAVQESSVSAGPEKDLEIVYMTDSGKGYEYYEGLSKKHSRSSSLGTNPSSWIGGQANWTDYPPPIDAQTKTREEHRRNNTLNNKNVVLKKLNPFEIFEMICNRNEEKGGYCSLTKVKMNDIVWCRQYMVKYFVMRTFRQHLDYKSMENNSYERHLFSFRDSGLDRNFSWACNAKLHE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should exhibit Polar residues compositional bias.2. The designed protein have F-box domains with specific sequence motifs that are crucial for its activity.3. The designed protein localizes to the mitochondrion. The designed protein sequence is ",
    "response#1": "PPNNPDYLRLQTAALDTTPERNFNDEFQAIKDLTTSTLQDRIEMERLFSKVVHEFSVTAASGAMSIFYSDFVAMNPESPTRDQIFLKDNIFYSYVSDVSGNYEGKGGDEAAIAASNQDLKTINILNRLHMHEVRYLLTTVVEFAGRRILAQTPVPGLLATMGNKIVKDANTGEEVTEDFVNDINVKYGLDEGLGKIVYDADFDSVLEKKFVKAFHLKKHKVNGTELAFSSQSKGIVGFDKRRYILDLANTYPLDINFD",
    "response#2": "ESVHGLICFRKAATPIIWNPTMRKFKPLRKLDERWKNIKVSLGYDPVDGKHKVVCMPYGNAFYECRVLTLGSDQEWRTVKTNHKNSPFTFHGGVCYRQSRCINGVIYYRADTNSGRVILSFDVRSERFDVIELPWDENFGLVMMMSYKGRLACLGFNHEKNSRSMWVLENVEQREWSCHTYLPISHYEPGLENYFNLTGITNDGELIYVPNTVLERFHVIYFR",
    "response#3": "DMARNQELLSLKNLEGQDDTREWSEDLQSLKELPRETINDRVVRERHLNKTYFDFTEAAVAGAVQVVHGEIQPINPSEPEASHIFLHKGIFYSVGADGSGTYAEIGGDEAARVASAKDLQAVQQLVQFDFPEIASLCTTVVDYQGKRIVCQSPVPGIFRQPENAPPQVKYGSVEGAEEIASEQEFGDAFKPIAAAFRLKTHTVKDANKEHSLHLASDCKGLAGTDGRKYLLDLYRLAPVDIAFS"
  },
  {
    "reference": "MASQKRPSQRHGSKYLATASTMDHARHGFLPRHRDTGILDSIGRFFSGDRGAPKRGSGKVPWLKQSRSPLPSHARSRPGLCHMYKDSHTRTTHYGSLPQKSQRTQDENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQKPGFGYGGRASDYKSAHKGFKGAYDAQGTLSKIFKLGGRDSRSGSPMARR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane .2. The designed protein should be involved in positive regulation of interleukin-6 production, negative regulation of heterotypic cell-cell adhesion, myelination by localizing to plasma membrane, cell periphery, cell projection, myelin sheath and exhibiting structural constituent of myelin sheath, protease binding, calmodulin binding. The designed protein sequence is ",
    "response#1": "LTIPFAFFLPELIFGFLVWTMVAATHIVYPLLQGWVMYVSLTSFLISLMFLLSYLFGFYKRFESWRVLDSLYHGTTGILYMSAAVLQVHATIVSEKLLDPRIYYINSAASFFAFIATLLYILHAR",
    "response#2": "LFLTIPFAFFLPELIFGFLVWTMVAATHIVYPLLQGWVMYVSLTSFLISLMFLLSYLFGFYKRFESWRVLDSLYHGTTGILYMSAAVLQVHATIVSEKLLDPRIYYINSAASFFAFIATLLYILHAFSIYYM",
    "response#3": "FLRTLPGFLIVAEIVLGLLVWTLIAGTEYFRVPAFGWVMFVAVFYWVLTVFFLIIYITMTYTRIPQVPWTTVGLCFNGSAFVLYLSAAVVDASSVSPERDSHNFNSWAASSFFAFLVTICYAGNTYFSFIAWL"
  },
  {
    "reference": "MKSLFLSEKIYVLILAGGTGTRMGSEIPKQFLEFSNEPILIHTLKKFQSWKKQKQIVLVSHPEFISETESICSPFLENQDCIIEGGETRHGSMLRGLSALTIQSEDILLIHDAARPFVLLKELDLLCENIRENGISTLASRTSETVLEESNGKTSSFLDREHIWFMKTPQGIRGDILKELLTLPMDPIPTDLCSWALTAGKKSSIVESHPFNLKITRKEDLELAEFYSDLFEKLR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that is capable of catalyzing reactions within the isopentenyl diphosphate biosynthesis via DXP pathway, isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 2/6 pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "LGEPDMRTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTALNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASC",
    "response#2": "LGEPDMRTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTALNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASQ",
    "response#3": "LGEPDMRTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTALNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASR"
  },
  {
    "reference": "MSELLLPPEHRYAKIIKEKLNEDGSELSILNLGPTHPATHGIFQNILLMDGERIVDAEPTIGYIHRAFEKIAENRPFYQITPLTDRMNYCSSPINNMAWWMTLEKLLDIEVPKRAQYLRVIVMELARITDHIICNSILGVDTGAYTGFLYVFQFREKIYEIYEEICGARLTTNMGRIGGFERDWSPKAFQLLNTFLEEFPIAWKEFENLFERNRIFIDRTVNVGAISAEKAMAYGFTGPNLRAAGIDYDVRVAEPYSSYEDFEFIIPVGKSGDTYDRFCVRNAEVWESLSIIRQALAKMPEGNVYHAEVPDYYLPPKEDVYHNMESLIYHFKIVMGEVPVPVAEIYHAVEGGNGELGFYLTTDGSRTPYRLHFRRPCFIYYQAYPEMIKGALLSDAIVILSSLNVIAGELDA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.; NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain2. The protein can be targeted to plasma membrane and perform quinone binding. The designed protein sequence is ",
    "response#1": "ELITLYLALELSSYSLYALIPLRGQDRRAAEAGIKYILFGAAVTALALFGLSYIIAAKHTTYLSGLAATSWSFADAPMAVIGLTLFLAGFFYKLALFPFHFWCPDVYQGAKNETAAYVATIPKLGAVVVLVRLAAFVAPHLEVTTILAILGAVSMTAGNLAALVQRDLKRLLGFSSVAHAGYVMLGLAAGSAAGMSAAAFYSLAYILMNLAAFYVVCAIAKNDENPSLDDLDGLYKRAPALAMILAVAAFSLVGLPPTAGFAGKLFLLSAAWDQGYHWLVIVAVLNTAISAINY",
    "response#2": "ELITLYLALELSSYSLYALIPLRGQDRRAAEAGIKYILFGAAVTALALFGLSYIIAAKHTTYLSGLAATSWSFADAPMAVIGLTLFLAGFFYKLALFPFHFWCPDVYQGAKNETAAYVATIPKLGAVVVLVRLAAFVAPHLEVTTILAILGAVSMTAGNLAALVQRDLKRLLGFSSVAHAGYVMLGLAAGSAAGMSAAAFYSLAYILMNLAAFYVVCAIAKNDENPSLDDLDGLYKRAPALAMILAVAAFSLVGLPPTAGFAGKLFLLSAAWDQGYHWLVIVAVLNTAISALNYWG",
    "response#3": "AVNLLLFYLFFEAILVPMYIIIGVWGGDNRIYAALKFFLYTFFGSVFFLLALIYIYSKIHSFDLTYILALTENIPLFAQKILWWTIFIAFAVKIPMIPFHTWLPDAHVQAPTSGSVILAGILLKLGSYGFLRVLLPLLPNVSQEFAIYVIYLSVIAIIYSSLVALAQKDIKQMIAYSSIAHMGYVTIGIFSFTETGISGAIFQMLSHGVVSSSLFLIVGTLYERLHTKEIAKYGGVASKMPVLATFFMITMLSSIGLPSTSGFIGEFLSLLGIYKVNVVAACIALF"
  },
  {
    "reference": "VLPPVLVPRHTEIPAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSMDAGSPNLSPNPMSPAHNNLDLQPVTYCEPAFWCSISYYELNQRVGETFHASQPSMTVDGFTDPSNSERFCLGLLSNVNRNAAVELTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSIRCSSVS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that can perform promoter-specific chromatin binding, transcription coactivator binding, sterol response element binding, DNA-binding transcription activator activity, RNA polymerase II-specific in cytosol, nuclear inner membrane, chromatin to support positive regulation of chondrocyte differentiation, negative regulation of inflammatory response.2. The designed protein have MH2 domains with specific sequence motifs that are crucial for its activity.3. The protein should exhibit Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "CMPQLVPTMLSLLKAIEPDTIYAGYDSTLPDTSTRLMTTLNRLGGRQVISAVKWAKALPGFRNLHLDDQMTLLQCSWLFLMSFSLGWRSYQQCNGNMLCFAPDLVINEERMKLPYMADQCEQMLKISSEFVRLQVSHDEYLCMKVLLLLSTVPKDGLKSQTVFDEIRMSYIKELGKAIVKREENSSQNWQRFYQLTKLLDSMHELVGGLLNFCFYTFVNKSLSVEFPEMLAEIIK",
    "response#2": "QEEEQAFLVSLYKFMKERHTPIERVPHLGFKQINLWKIYKAVEKLGAYELVTGRRLWKNVYDELGGSPGSTSAATCTRRHYERLVLPYVRHLR",
    "response#3": "EEEQAFLVSLYKFMKERHTPIERVPHLGFKQINLWKIYKAVEKLGAYELVTGRRLWKNVYDELGGSPGSTSAATCTRRHYERLVLPYVRHLKC"
  },
  {
    "reference": "MVDDNGSSPEVEGAEGAGAPLLALLRVDGLNQTQTRSPSPSFFGSYNISEDVYFYFNGLPTSTELVLNATTSATSATLSPAMVATGGGGTTTPEPDLSEFLEALPNDRVGLLAFLFLFSFATVFGNSLVILAVIRERYLHTATNYFITSLAVADCLVGLVVMPFSALYEVLENTWFFGTDWCDIWRSLDVLFSTASILNLCVISLDRYWAITDPFSYPMRMTVKRAAGLIAAVWICSSAISFPAIVWWRAARDGEMPAYKCTFTEHLGYLVFSSTISFYLPLLVMVFTYCRIYRAAVIQTRSLKIGTKQVLMASGELQLTLRIHRGGTTRDQQNQVSGGGGGGGGGGGGGGSLSHSHSHSHHHHHNHGGGTTTSTPEEPDDEPLSALHNNGLARHRHMGKNFSLSRKLAKFAKEKKAAKTLGIVMGVFIICWLPFFVVNLLSGFCIECIEHEEIVSAIVTWLGWINSCMNPVIYACWSRDFRRAFVRLLCMCCPRKIRRKYQPTMRSKSQRFATRRCYSTCSLHGIQHVRHNSCEQTYI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should contain a Helical; Name=6, Helical; Name=7, Helical; Name=3, Helical; Name=1, Helical; Name=4 membrane-spanning region.2. The designed protein should have tyramine receptor activity, adrenergic receptor activity, dopamine neurotransmitter receptor activity, G protein-coupled receptor activity, G protein-coupled amine receptor activity within membrane, synaptic vesicle membrane, plasma membrane to regulate adenylate cyclase-activating adrenergic receptor signaling pathway, dopamine receptor signaling pathway, G protein-coupled receptor signaling pathway, response to odorant, cellular response to dopamine.3. For general function, the protein need meet that This receptor is an attractive candidate for initiating biochemical cascades underlying olfactory learning.; The activity of this receptor is mediated by G proteins which activate adenylyl cyclase; In terms of antagonist responses, would be classed with the D1-like dopamine receptor group; Also capable of generating a calcium signal; Receptor for dopamine4. The Cytoplasmic, Extracellular topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells. The designed protein sequence is ",
    "response#1": "GNVLVCAAIVHSRHLRAKMTNVFIVSLAVSDLFVALLVMPWKAVAEVSGYWPFGAFCDIWVAFDIMCSTASILNLCIISVDRYWAISRPFCYERTMTPRVALVMVGLAWTLSVLISFIPVQLHWHRDKVGSWGGVDPLSHLANGTPWEEAGEPDVREENCDSSLNRTYAISSSLISFYIPVAIMVVTYTRIYRIAQGQIRRISCLERAAEHAQSCRSREACAPDTGLRASIRKETKVLKTLSVVMGVFVCCWLPFFILNCMVPFCGGPPEDFPCVSETTFDVFVWFGWANSSLNPIIYTLRNQEMKAAIKKVWSKLLL",
    "response#2": "GNLLIVVTVTVSETLGSPMSFFLAGLTFIDIIYSSSISPRLISDLFFGNNSISFQSFMAQLFIEHLFGGSEVFLLLVMAYDRYVAICKPLHYLVIMRQWVCVLLLVVSWVGGFLQSVFQLSIIYGLPFCGPNVIDHFFCDMYPLLKLACTDTHVIGLLVVANGGLSCTIAFLLLLISYGVILHSLKKLSQKGRQKAHSTCSSHITVVVFFFVPCIFMCARPARTFSIDKSVSVFYTVITPMLNPLIYTLRNQEVRSALKRCICR",
    "response#3": "GNGLVCAAVLRERALQTTTNYLVVSLAVADLLVATLVMPWVVYLEVTGGVWNFSRICCDVFVTLDVMMCTASILNLCAISIDRYTAVVMPVHYQHGTGQSSCRRVALMITAVWVLAFAVSCPLLFGFNTTGDPSICSISNPDFVIYSSVVSFYVPFGVTVLVYARIYIVLRQRQRKRILTRQNSQCISIRPGFPQQSSCLRLHPIRQFSIRARFLSDATGQMEHIEDKQYPQKCQDPLLSHLQPPSPGQTHGGLKRYYSICQDTALRHPSLEGGAGMSPVERTRNSLSPTMAPKLSLEVRKLSNGRLSTSLRLGPLQPRGVPLREKKATQMVVIVLGAFIVCWLPFFLTHVLNTHCQACHVSPELYRATTWLGYVNSALNPVIYTLTNKEMRQAFYKLFRRH"
  },
  {
    "reference": "MRPSGRKLDQMRSVSIEPNVMKHAEGSCLIRMGETHVLCSASIEDKPPPFLKNTGLGWVTAEYGMLPRATTSRNRREAAAGKQSGRTQEIQRLIGRALRAGVDRSALGERQIVIDCDVLQADGGTRCASITGGWVALRLAVNKLLKAGIIVSDPIVDHVAAVSCGIYAGQPVLDLDYAEDSTAGTDGNFVLTGRSRMIEVQMSAEGASFSRDEMGQLLDLAEAGIAELVAAQKAALG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein have enhanced phosphate + tRNA(n+1) = a ribonucleoside 5'-diphosphate +   tRNA(n).2. The protein must be able to tRNA binding, phosphate-dependent exonuclease activity. The designed protein sequence is ",
    "response#1": "GLVAAISVGIYQDEVLLDLCYEEDSNCQTDLNVVMTQAGEFIEIQGTAEEKPFTRTQANAMLEVAEKA",
    "response#2": "GLVAAISVGIYQDEVLLDLCYEEDSNCQTDLNVVMTQAGEFIEIQGTAEEKPFTRTQANAMLEVAEKA",
    "response#3": "FLAATSVGIDGEHGIILDLNYIEDSKAQVDMNIVMTGSGRFVEIQGTGEEATFSYEQLLDLLTVAEKL"
  },
  {
    "reference": "MLKTYNVAIMGATGAVGQQMKEQLEKRKFPIKDIVFLSSSRSAGKEIEFNGKSYTVQEAKPESFEGIDIALFSAGGSVSEKFAPEAVKRGAIVIDNTSAFRMDKEVPLVVPEVNKDDLRLHKGIIANPNCSTIQMVCALQPLKELYGMTKVVVSTYQAVSGAGIDAINELKSQAADFDNAKNAEAKVLPVKSAEHHYPIAFNVVPQIDQFTDNGYTFEEMKMINETKKILHDDSLAVAATCVRLPVVTGHSESVYVELEKAATVDQVKQAMIDAPGVELMDDPSNQIYPMPLMTAGLDDVFVGRIRQDLDNPNGFHLWVVSDNLVKGAALNSVQIAEALIEENII",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Proton acceptor, Acyl-thioester intermediate active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The protein contains novel Semialdehyde dehydrogenase NAD-binding domains that confer a unique function or activity.3. The designed protein must possess protein dimerization activity to support 'de novo' L-methionine biosynthetic process, threonine biosynthetic process under certain conditions.4. The designed protein should interact with enzymes and substrates within the Amino-acid biosynthesis pathway to enhance or regulate its activity.5. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..6. The substrate, NADP(+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MSNLAVVGATGAVGRIVLEQLALRKLPINRLKLLASQRSVGKEVRFADETLKVQLLEPDAFQGIDIVVASTPDEVSAEFASWATDAGAVVVDESGYWRMDPKVPLVIPEVNPEAIHDLKPINDAVGIKRVNVSTYQSVSGAGRLSMELLTVQSRALLNGGEVPPQSNQEKSVAFNAVPKIDRLMDNGYTKEEMKMVWETRKILEDPDILVNPTCVRVPVYLGHSEALHIETREKLSVGEARDLLDAAPGVVVLDGENPGDYPTATTEAANRDAVYVGRIREDVSHPRGINLWVVADNLRKAKAGIIANPNCTTILMAVAVWLYPLHRAFGVKRIFASSYQAVSGTGAKAIEELERQVGEVVAGKPVTREVYPHQIAFNVLPHVDSFLPSGYTKEEMKMENEGRKIMHHPGFRASVTCVRVPVYRAHSVAVSAEFEKPVTTAAALEVLRKAPGLDVVDDPSKNTYPLPLNAAEQYNCHVGRLRMDCALENGLCFWVVGDQLLKGAALNAVQIAEHLVAER",
    "response#2": "MTNLAVVGATGAVGRIVLEQLALRKLPINRLKLLASQRSVGKEVRFADETLKVQLLEPDAFQGIDIVVASTPDEVSAEFASWATDAGAVVVDESGYWRMDPKVPLVIPEVNPEAIHDLKPINDAVGIKRVNVSTYQSVSGAGRLSMELLTVQSRALLNGGEVPPQSNQEKSVAFNAVPKIDRLMDNGYTKEEMKMVWETRKILEDPDILVNPTCVRVPVYLGHSEALHIETREKLSVGEARDLLDAAPGVVVLDGENPGDYPTATTEAANRDAVYVGRIREDVSHPRGINLWVVADNLREHKIIANPNCSTIQMVVALRPLSLLAPIKRVVVSTYQSASGAGAEAMKELKNLTQDVLDGKTSKSKVLPYSLAFNLFLHNSPLAENDYCEEEMKMINETRKIMESPELSLTATCVRVPVLRAHSEAVNIEFVEPIEVRLARKALNDAPGIKIIEDFHANRFPMPVDVAGQDSVAVGRIRQDVSHANCLELWLCGDQIRKGAATNAIQIAELLIRDYL",
    "response#3": "MTTVAVLGATGAVGNELLQLLEERSFPIDQLKLLASERSAGKIKLWNGRNITIHPASPEQFQGVDLVFASAGGSISKKWRNSINAAGAVLIDNSSAFRMDPEVPLVVPEVNPEKDALTTAKGIIANPNCTTMAAMPVLYPLHRAFGVKRIFASSYQAVSGTGAKAIEELERQVGEVVAGKPVTREVYPHQIAFNVLPHVDSFLPSGYTKEEMKMENEGRKIMHHPGFRASVTCVRVPVYRAHSVAVSAEFEKPVTTAAALEVLRKAPGLDVVDDPSKNTYPLPLNAAEQYNCHVGRLRMDCALENGLCFWVVGDQLLKGAALNAVQIAELVALN"
  },
  {
    "reference": "MRIGIVGATGQVGSVMREILAERKLPVDQLRLFASARSAGRTLPWQGGEVTVEDAATADYSGLDVVLFSAGGSTSKAIAEKVAAQGPVVIDNSSAWRLDPEVPLVVSEVNPEAARRRPKGIIANPNCTTMAAMPVLRPLHEEAGLSGLVVATYQAVSGSGLAGVAELNEQASKAIETDATRLTFDGDAVDFPQPEKYDRPIAFNVLPMAGSIVGDGLDETDEEQKLRNESRKILDIPGLKVSGTCVRVPVFTGHSLQVNARFERAITPQRARELLAAAPGVELSDIPTPLQAAGKDASYVGRIRADETVEHGLALFVANDNLRKGAALNAVQIAELVAAE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a binding site that is specific to the target phosphate, NADP(+), substrate ligand.2. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.3. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.4. A protein with protein dimerization activity, NAD binding, aspartate-semialdehyde dehydrogenase activity is needed to enhance diaminopimelate biosynthetic process, 'de novo' L-methionine biosynthetic process, threonine biosynthetic process, isoleucine biosynthetic process in a specific context.5. The protein contains novel Semialdehyde dehydrogenase NAD-binding domains that confer a unique function or activity.6. A protein that is capable of catalyzing reactions within the L-threonine biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MINLGIVGATGQVGVAVREILLERDFPIGQVRFFASARSAGTVLSFGDREITVEDASSADPAGLDIAIFSAGATTSRALAEKFAEAGVIVIDNSSAFRKDPAIPLVVSEVNPSAMDGALKASPPKGIIANPNCTTMAAMPVLKPLHDRARIKRVVVSTYQSVSGAGKDGMDELWDQTKGMYVPGQEKEPSKFTKQIAFNVIPHIDTFLEDGSTKEEWKMVAETKKIIDTSILVTATCVRVPVFVGHSEAINIEFEDFLDEDEARDILREAPGVMVVDKREDGGYITPVECVGDFATFISRIRQDSTIDNGLNLWCVSDNLRKGAALNAVQIAELLVKE",
    "response#2": "MIKVGFVGWRGMVGSVLMDRMREENDFANIDPVFFTTSQHGKPGPIVSSDPGKPVAALQDAYSIEALAAMDIIISCQGGDYTHKVIEPLRESGWNGYWVDAASSLRMKNDSVIVLDPVNRPVIDQALAKGGRNWIGGNCTVSLMLMALAPLHKRYGIDKVVVSTYQAVSGSGHKAIVELENQMKDANYPNNVYPKRIATNCIPHIDVFNDNGFTKEELKMVYETQKILEDNKIKVNSTTVRIPVFFSHSESVYVEFKETPEVSEVKQILSEAPGVIVLDNPEKNEYPTPLEVANSDDTYVGRIRKDLFNPNALSMWVVADNLRWGAAEPLRRMLRILLER",
    "response#3": "MINIGIVGATGAAGGTALKLLLERDHPADKITLMASARSAGRKIQYGDDNIVISEASSDSFHGIDVAIFAAGGLVSRRLAPRAVEQGAFVIDKGSIFRMDPSIPLVVPEVNGDDIAWHEGHVNCTCVRVPVMRSHLAPLHKRYCLEYFSAVTFQSVSGTGKQALVELDECARAILEGKSFEPQVYPLPIGFNVLPHIGAMDESGVSDEEWKMLNESRKILHHQKLQVSCTTVRVPVKRGHSEAIVAQFEERVTLEEANAILSEAPGIIVMAENKYITPLQAAGSDLVYVCRLRSDLGMSRALSMWVVSDNLRISPIRSMIASTYQAVSGAGAGGPAELMQEVEALARGEAAEPQVFPYQIAYNVIPQIGGESTDGYTSEEMKMQNEGRKIMHLPGLLVNCTCVRVPVVRSHSISVTVQTERPVSVEEARAAIAKAPGCRLADDLAGRIYPMPLETSDQDLVFVGRIRRDLTHENGLAIWCCGDQVRGAGGTPLKPIMDKFGIDMVNITTMQAVSGAGYNGVSSMAILDNIIPYIGGEEEKMETESLKILGYVDEDNKFIYANFKIGVSCNRVPVIDGHTESIFVKTLEDANPEEILKVMDEFDPLKDYNLPTYAKPIVIKEGIDRPQPRLDRNNGNGMSITVGRIRRDNIFTVKYTALEHNTVTG"
  },
  {
    "reference": "MRVLTTIEGLRCWRTQQTGSVGFVPTMGALHTGHLSLIERARAENQWVIVSIFVNPLQFGPSEDLDRYPRPLEKDRALCEAAGADAIFVPTSSVLYGQLQPAPATLMQVVPPAAMLEVLCGPFRPGHFQGVATVVTKLLNLVQPDHAYFGQKDAQQLAILRRLADDLNLSVKIIGCPIVRELDGLALSSRNQYLDGQARQQAAILYRGLSAAKAKFAAGARSQRELVQAVQTVVKAEPAVQLEYVELVHPLTLTPLDAIETIGMLAVAAQLGNTRLIDNVTLRSRRPIIAIDGPAGAGKSTVARRLARQLGLLYLDTGAMYRALTWFVLSQGADPQEQVAVAELLSRCDIRLTTQAAGEAGRSLPQQVWVNGQEVTEAIREPEVTAQVSAVAAQAAVREALVQQQKQYGAEGGVIADGRDIGTHVFPHAELKVFLTASVQERAQRRQRDLAERGQPVPDLAELEQLISERDRKDSTRAISPLQKASDAIEIDTDMLSIEAVIDKIVALYTERVVQSA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must have high specificity towards ATP binding. The designed protein sequence is ",
    "response#1": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#2": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#3": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR"
  },
  {
    "reference": "MMNTNLDLLATLDQIGQPNILVIGDFMLDRYTWGNAERVSQEAPVLLLQVDKEEEKLGGAGNVCQALSHLGARVMAAGILGSDFSGDSIRSMLTEIGVDCELLAADPKRPTTTKNRFMGRAANRHPHQILRVDRETHTEMPPALIGTMIHKIEKKIADIDAILLSDYDKGSLSPLLVRRVXDLARQQQIPIIVDPAASRDYTLYRGATLMTPNRSEAEHATGMTINDPLDAILSARSICAIAELQMAIVTIDKDGMVLAERNGEGNIFPIDPRSVYDITGAGDIVLATVGLCIAAKKSGPIAARLANIAAGIAVEQVGAAMIEREELRQRLIAXRPPMENKLVELLHAKKIVDVHRATGKRIVFTNGCFDLLHVGHITNLHQAAELGDLLVVGLNSDHSVRQLKGDNRPVIGQRDRAAILAALQCVDYVIIFNQDAPLELIEVLRPDVLVKGGDYEKSQIVGKELVESYGGIVITTPLVEGVSTTRILEAASV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein contains novel Carbohydrate kinase PfkB domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "PNNLREELTRYLFVLQLKQDILSGKLECPFDTAVQLAAYNLQAELGDYDFAEHSPELVSEFRFVPIQTEEMELAIFEKWKEYRGQTPAQAETNYLNKAKWLEMYGVDMV",
    "response#2": "ARENELIAQLAQQRGKQSSGGSAANTLVSLAQLGGTGFYACKVGKDEAGAFYLQDLNDCGLDTNPHHETAGEGITGKCLVFVTPDADRTMNAFLGISGSLSVTEMDWSALKQSQYLYLEGYLVTSPSAKAACIEAKAIAEQSGVKTCLSLSDPNMAKFFQDGLKEMLGSGVDLLFANEAEALEMAGTSDLNQAIAYCKSIAKNFALTRGGAGSLIFDGENLLTIGTPKVQPIDTVGAGDMYAGGFLYGLTHGMDYEKAGQLASETAAKVVTCYGPRLDARENELIAQLAQQRGKQSSGGSAANTLVSLAQLGGTGFYACKVGKDEAGAFYLQDLNDCGLDTNPHHETAGEGITGKCLVFVTPDADRTMNAFLGISGSLSVTEMDWSALKQSQYLYLEGYLVTSPSAKAACIEAKAIAEQSGVKTCLSLSDPNMAKFFQDGLKEMLGSGVDLLFANEAEALEMAGTSDLNQAIAYCKSIAKNFALTRGGAGSLIFDGENLLTIGTPKVQPIDTVGAGDMYAGGFLYGLTHGMDYEKAGQLASETAAKVVTCYGPRLDLVVGDFMLDQHVYGDAERLSADAPVPVLQVRRHESTPGGAANLCADLAAMKGTVIACGLIGCDAEADELKAALARLHIDTAALVADASRPTTLKRNFIGLAQARHPQKMFRVDYESQEPIGGQALSALLAHVARVVRSVDIVCIEDYGKGVCTEHVCQEVIRLARQAGKQVLVDPANNADYLRYRGATAITPNRTEAEKATGLKTHDHDSAHNAALARGLLARLELDAVILTLDRHGALLLERSNDLPLHVPTVAREVYDVTGAGDMLLAGLAAARANHLDWPDAVRLANAAAGLEVEVFGVKP",
    "response#3": "PNNLREELTRYLFVLQLKQDILSGKLECPFDTAVQLAAYNLQAELGDYDFAEHSPELVSEFRFVPIQTEEMELAIFEKWKEYRGQTPAQAETNYLNKAKWLEMYGVDMI"
  },
  {
    "reference": "MLTHLSFKGFLGRRLQLGVCGSIAAYKALDLVRMFRDTGTDVGATLTDSARQFVTPLSFEALGASPVFSAMYPVGDDVFGHLTPGEAAHAMLIAPATASTLARLANGLADDMLSCQALAFPKPLVIAPAMNPRMWHNAATQANWETLKARGHVCIEPGCGRTACMEEGRGRLADLNEIFLYGLRALTPQDMAGQTVMITLGPTREKWDGVRFWSNPSSGTMGACFAVAAWLRGATVHAVTGPGTPWLPSMISRYDVTSAKEMFEAAEGLWPTMDIGVFTAAVADFSPIPFGDEKFKKGAEELVVRFASTTDILKTLGAAKKEGQRIVGFAAETSSMQENALKKLRAKNADMVVGNIVGRADSGFQSPTNEVFVTDRNGRQEEWPVQPKTEVAWRVLDWLLQL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.2. The presence of Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.3. A protein should be able to manipulate the activity of enzymes in the Cofactor biosynthesis pathway, either by activating or inhibiting their function.4. The protein must have high specificity towards phosphopantothenoylcysteine decarboxylase activity. The designed protein sequence is ",
    "response#1": "MRVALAVSGGIAAYKAAEVLRGLQRAGCRVRVAMTERATEFVTPLTFRALSGEHVIVDDYAPDNPDPIAHITFSQTVDLLLVAPATANIIAKFAHGIADDFVTSTYLACTAPILIAPAMNTTMWNHPATARNLRQLRADGVHVVEPDAGEMACGTIGPGRLSDPALIVAAIEATLRGGGGADLEGLHVAVSAGGTREPLDPVRYLGNRSSGRQGWALARVAAQRGARVTLVAAHTADLATPAGVDLVRVGSAEQLRVAMHEVAASADVVVMAAAVADFRPVATAGHKIKKTDRDPEPVALTRNADILAELVTARRSGQVVVGFAAETGDEGADVLEYGRAKLKRKGCDWLVVNAVGDGKAFEVEDNSGWLLDARGAEIAVAQGSKGALAHALWDEVVRRLS",
    "response#2": "LRDAGSDVRVVMTRGARAFVTPLTFQAVSGNPVRTELLDPDAEAGMGHIELARWADRILVAPATANFMARFAQGLADDLLSTLCLATTAPVALAPAMNRQMWAHPATQANCAVLEERGVRLIGPESGSQACGETGPGRMTEPAELVAALRDAGSDVRVVMTRGARAFVTPLTFQAVSGNPVRTELLDPDAEAGMGHIELARWADRILVAPATANFMARFAQGLADDLLSTLCLATTAPVALAPAMNRQMWAHPATQANCAVLEERGVRLIGPESGSQACGETGPGRMTEPAELVAAAEAVLAG",
    "response#3": "MKVIVGVAGGIAAYKACHLVRNFKEAGDEVRVIPTPSALNFIGAATFEALSGNSVSTSVFEGVDEVQHVRLGQEADAVVIAPATADLLARLAAGRADDLLSATVLVATCPVIVAPAMHTEMWLNPATQANVHTLRSRGVTVLEPASGRLTGVDSGPGRLLEPEQIAELLA"
  },
  {
    "reference": "MSVVSLPQMLEAGVHFGHQTRRWNPKMRRYIFTDRNGIHIIDLTQTAHLLDEAYSYLREASDQGKKILFVGTKRQAAPVIAQEAKRCGMFWVNQRWLGGMLTNWDTIRTSVDQLKELETMEANGTLDLLPKKEASVTRKKLERLTKYLGGLKNMRRKPDILLVVDQRREQNAVMEARRLNIPIVSLLDTNCDPDLTDVGIPANDDAIRSIRLIVGKLADAIYEGRHGQLEDFSEEEPVPAAAPVAAVAVAVAAPAEAESEDKGEVLYSFDDEEE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design must be focused on enhancing the efficiency of translation. The designed protein sequence is ",
    "response#1": "DETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAMV",
    "response#2": "YDCYANLALLKLYQFNPHLSRDETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAKGTVVREI",
    "response#3": "DETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAG"
  },
  {
    "reference": "MTLLETIGIAAVTLHALLSAIYKSMQAFYARKASGSQPRSKDIDPAALPSVDIIVPCFNEDPAILSACLSSLAGQDYGGKLRIYMVDDGSCNREAILPVHDFYTSDPRFEFLLLSKNVGKRKAQIAAIERSCGDLILNVDSDTSIASDVVTLLVEKMRDSDVGAAMGQLKASNRDKNLLTRLIDMEYWLACNDERAAQARFGAVMCCCGPCAMYRRSALLLLLDQYQTQLYRGKPSDFGEDRHLTILMLSAGFRTEYVPEAIAKTVVPDRMGSYLRQQLRWARSTFRDTLLALPLLPSHNRFLTLDAIHQNIGPLLLAVSSATGITQFILTATVPGWTIIIIASMTMVRCSVAAYRSRQIRFLAFSLHTLINLFMLIPLKGFALLTLSNSDWLSRGSTTDGPAIAESNAASNEAEIVASASPFGGGTSWRFRR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have a specific function within the plasma membrane. The designed protein sequence is ",
    "response#1": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPR",
    "response#2": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPM",
    "response#3": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPV"
  },
  {
    "reference": "ADVPGNYPLDKDGNTYKCFLLGGNEECLNVCKLHGVQYGYCYASKCWCEYLEDDKDSV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that localizes to extracellular region and can perform toxin activity with high specificity and efficiency.2. For general function, the protein need meet that Moderately toxic, but very high abundant; Does not produce effect when administered to blowfly and cabbage looper larvae3. The designed protein have LCN-type CS-alpha/beta domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "RDGHIARNNNCVYVCFIEYTYIGTSRINCNDLCTKNGAKSGYCHQFSEYGNSCWCIDLPDNVPIKVPGDCR",
    "response#2": "LDGYPLSKNNYCKIYCPDEKVCKWSCKHRAGATNGKGDCINKGCYCYDVAPGTEMYPGRLPCQ",
    "response#3": "RDGHIARNNNCVYVCFIEYTYIGTSRINCNDLCTKNGAKSGYCHQFSEYGNSCWCIDLPDNVPIKVPGDCR"
  },
  {
    "reference": "MSSYKVGILGATGAVGQQMLTVLEERHFPISELRLFGSARSAGKKVTFAGQEVTIQEAKVEAFKGLDLLLGAVENDLAKQFLPEAVKLGVQVVDNSSAFRLDPNCPLVIPEVNPEDVRLTKGLIANPNCATIIALTAVMALHRYARIRRMVVSTYQAVSGAGKAGIDELLDQVKAPEGTTPEMKVFPYPIAYNLIPQIGGFNDLGYSSEEMKLQNEGRKMLHDDTLRVACTCVRVPIIRSHSEAITLEFEREISPEKARELLANAPGVVLCDDPNAQKYPMPLLTSDQDLVYVGRVRRDLSASAELYNKSLTLFCCADQVRKGAATNAVQIAEILFNVA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should consider the endogenous proteins and enzymes present in the Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5 pathway to avoid interference with native metabolic processes.2. The designed protein should have NAD binding, protein dimerization activity to facilitate 'de novo' L-methionine biosynthetic process, diaminopimelate biosynthetic process, lysine biosynthetic process via diaminopimelate.3. The NADP(+), phosphate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "LAGLFEQGWVEWVTSQTYQAASGAGAKNMRELVNQMRIIGENCENLLDTPSTAILDIDRNVINTLRNPAFPTENFGAPLAASLIPWIDRAMENGQTREEWKGISETNKILGREENPVPIDGCCVRVGSMRCHSQAYTIKLNRDVPMADIEDAIKKQNDWVYLVANNKEETLKELTPARVTGTLDIPVGRLRKMNLGPQYLSAFSVGDQLLF",
    "response#2": "LAGLFEQGWVEWVTSQTYQAASGAGAKNMRELVNQMRIIGENCENLLDTPSTAILDIDRNVINTLRNPAFPTENFGAPLAASLIPWIDRAMENGQTREEWKGISETNKILGREENPVPIDGCCVRVGSMRCHSQAYTIKLNRDVPMADIEDAIKKQNDWVYLVANNKEETLKELTPARVTGTLDIPVGRLRKMNLGPQYLSAFSVGDQLLS",
    "response#3": "LKALHDAFGLVGFTATSYQAASGAGQAGVDEFMEQQQALAPDPDLLDRDGDRARAKVAEPQVHADVLAFNVVPRLGGVDDEGYTDEERKLERESRKILSLPDLAVAPTCVRVPVVTGHAVAVRAVFADPVRSQAEAVASMDHFPGLVVEQAPTPLAWAGRDDIAVGRVRCDLVDDHALSFVVVGDNLRT"
  },
  {
    "reference": "MKPLKTVRKAITVATLCALGATAAATHAEAPDNDYWWPNRLSLEPLRDASASADPMGADFDYAEAFETLDVKALEKDLRALMKDSQDWWPADFGHYGPFFIRMSWHAAGTYRTIDGRGGADGGMQRFAPLNSWPDNASLDKARRLLWPIKEKYGNAVSWADLIVLAGNMAMDDMGFKTLGFAFGRADEWQPEDVNWGPEGEWLTDQRRNEMGRIEGPFGATEMGLIYVNPEGPHKNPDPLKAAQAIRDAFGRMAMNDEETAALIAGGHTFGKAHGAHKAADCVGPEPEAAPLEQQGLGWANKCGTGKGSDAVTSGLEGAWTINPVQWTHNYLQNLYGFEWELHIGPGGAQQWRPVDGQGASLVPDAHDPDKRHAPMMFTTDIALKEDPSYRKITQRWLENPEEFEQAYARAWFKLIHRDMGPASRYLGPWAPEESFIWQDPVPEADYKQVSDKDISRLKSEILDTGLSVGELVRTAWASASTFRETDMRGGANGARIRLMPQKDWAVNEPADLAKVLAKLEKVQADFNKKSRKTKVSLADVIVLGGAAAIEKAAADAGYKVSVPFVPGRTDATQEMTEVASFTVLEPKSDGFRNYHAASYDHPPAQALVEKAALLDLTVPEMTALVGGMRVLGANVGGNSAGVFTDEPGKLTTDFFVNLVDMSTKWQKASTPGLYEGVDRDSGEVKWTATPVDLIFGSNSELRAIAEFYAMDDSKKKFVDDFIAAWTKVMTADRFDV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have a high affinity for heme b. The designed protein sequence is ",
    "response#1": "VSMADLIVLAGCAGVEQAAKAGGHQIEVPFTSGRGDASAEQTDAESFAVMEPVMDGFRNYQARELSTSPEEMLVDRAQLLGLSAPEMTVLVAGLRVLGANHGGSAHGVLTNRPGVLSSDFLTNLLDMGTEWKPSGKGVYEGRDRASGAARWTATRVDLVFGSNSQLRALAERYAQDDAEASFVADFVAAWVKL",
    "response#2": "VSMADLIVLAGCAGVEQAAKAGGHQIEVPFTSGRGDASAEQTDAESFAVMEPVMDGFRNYQARELSTSPEEMLVDRAQLLGLSAPEMTVLVAGLRVLGANHGGSAHGVLTNRPGVLSSDFLTNLLDMGTEWKPSGKGVYEGRDRASGAARWTATRVDLVFGSNSQLRALAERYAQDDAEASFVADFVAAWVKL",
    "response#3": "VSMADLIVLAGCAGVEQAAKAGGHQIEVPFTSGRGDASAEQTDAESFAVMEPVMDGFRNYQARELSTSPEEMLVDRAQLLGLSAPEMTVLVAGLRVLGANHGGSAHGVLTNRPGVLSSDFLTNLLDMGTEWKPSGKGVYEGRDRASGAARWTATRVDLVFGSNSQLRALAERYAQDDAEASFVADFVAAWVKL"
  },
  {
    "reference": "MKTVLIASNNAHKAVEIAEALDFPGWEFKTLKQAGVHSDPVEDAETFLGNARIKAQAAQQASGGMAVLADDSGLEVDALDGAPGVHSARYAGEPCDDAANNAKLLDALSDVEDEQRSARFVCHLVYLDEQGREHDARGTVEGRIAHEPQGDNGFGYDPLFLPEVFDFERSLGQALSEEKNSISHRGNALRELRAKLSENA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+).2. The protein should be able to bind Mg(2+), substrate ligand in a variety of conditions3. The designed protein should be optimized for XTP diphosphatase activity, metal ion binding, ribonucleoside triphosphate phosphatase activity, dITP diphosphatase activity, ITP diphosphatase activity activity in order to improve purine nucleoside triphosphate catabolic process, nucleotide metabolic process.4. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.5. The protein requires Mg(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "MEIIFATNNQHKTDEIRAILGERFTLLSLHDIGCTEDIPETADTIEGNAILKARYVQEHYGYDCFADDTGLEVEALNNEPGVYSARYAGSRDNAQNIEKLLTELQGHTDRSAQFRTVIAFVLSGTEHRFTGICEGTITTTPRGDKGFGYDPIFIPDGYTQTFAQLPMEVKNTISHRGRATQKLIAFLKTL",
    "response#2": "MKKVLVASNNPGKLREIRAILGEDFELVTQQELGVTDAPEPHDTFVENALAKARHASRLTGLPALADDSGLEVDYLQGAPGIYSARYAGEGATDAANNAKLLRELGDKADRRAHYYCVVVFLRHADDPAPLICQGVWHGRILSEPKGEGGFGYDPLFWVPEQGCSSAELDPALKNRLSHRGQALKQLLQQLQAQ",
    "response#3": "MSILSGKVAIVTGSGRGIGAAMAIRFAREGAAGVVVNDYGVATEDGANTQAAEAVVNEITAAGGRAAANYDSVAVPGGAQIVVTALDAFGTVDIVVNNAGILRDRMIYRMSERDWDAVVAVHLTGAFHVSKAAWPHMKAQNFGRVINLS"
  },
  {
    "reference": "MDPVDPNLEPWNHPGSQPKTACNNCYCKRCSWHCQICFLKKGLGISYGRKKRRPRRGAPHSNKDHQNPLSKQPLPQTQREQTGPEESKEKVESKAEPDRFA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to promote processive transcription elongation by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts; In the cytoplasm, Tat is thought to act as a translational activator of HIV-1 mRNAs.; This binding increases Tat's affinity for a hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA) and allows Tat/P-TEFb complex to bind cooperatively to TAR RNA; Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication; Other factors such as HTATSF1/Tat-SF1, SUPT5H/SPT5, and HTATIP2 are also important for Tat's function2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.3. A protein that contributes to positive regulation of transcription elongation by RNA polymerase II, modulation by virus of host chromatin organization by functioning in extracellular region with protein serine/threonine phosphatase inhibitor activity, protein domain specific binding, RNA-binding transcription regulator activity.4. Incorporating a Nuclear localization signal, RNA-binding (TAR), and protein transduction motif is critical for the successful design of the protein. The designed protein sequence is ",
    "response#1": "MEPVDPRLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFTKKALGISYGRKKRRQRRRAPQDSQTHQASLSKQPTSQPRGDPTGPEESKKKVERETETDPVD",
    "response#2": "MDPVDPNLEPWNHPGSQPKTPCTKCYCKKCCYHCQKCFLTKGLGISYGRKKRRQRRRAPQGDQAHQVSIPKQPSSQPRGDPTGPKESKKKVEGKTETDQFD",
    "response#3": "MEPVDPSLEPWKHPGSQPKTACNNCYCKKCCYHCQVCFTKKGLGISYGRKKRRQRRRTPQGSETHQVSLSKQPASQPRGDSTGPKESKKKVERETETDPVD"
  },
  {
    "reference": "MAVPKKRTSASKKRIRKNSWKGKGYGTALKAFSLGKSLSTGNSKSFFVPKNSLKVFKRIIKNWNNLNRLGSKN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that contributes to translation by functioning in large ribosomal subunit with structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "MP",
    "response#2": "GRQAMPFKCMQLTDFILKFPHSARQKYVRKAWEKADINTKWAATRWAKKIDARERKAKMTDFDRFKVMKAKKMRF",
    "response#3": "GTSSFGKRRNKTHTLCRRCG"
  },
  {
    "reference": "MAFKLNIGLLALSLSLSLVHLDGVRAGMATRYWDCCLASASWEGKAPVYAPVDACKADGVTLIDSKKDPSGQSGCNGGNKFMCSCMQPFDDETDPTLAFGFGAFTTGQESDTDCACFYAEFEHDAQGKAMKRNKLIFQVTNVGGDVQSQNFDFQIPGGGLGAFPKGCPAQWGVEASLWGDQYGGVKSATECSKLPKPLQEGCKWRFSEWGDNPVLKGSPKRVKCPKSLIDRSGCQRKDDNTISPYSGKVDSANTAAPAQYKRDRSVCLAGGKKGKSAAGGVDGSGDASGGADASGAGGAAEGSQGQPEGYGQPSGGNDQGSSNGDATTGAGSGSGSDSGSTANGSGSGAPTSGSDGSAVAPPSGGSNPGAAQGGQGGAQPGPSGGHKKCHKKH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The ideal protein would be one that plays a critical role in cellulose catabolic process through cellulase activity within extracellular region.2. The designed protein is the optimization of the Nucleophile to achieve high catalytic efficiency for Endohydrolysis of (1->4)-beta-D-glucosidic linkages in   cellulose, lichenin and cereal beta-D-glucans..3. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "PTNDEFFVEAGINASGTNFIEIKAIVNNQSGWPARATNKLKFRYFVDLSELIKAGYSPNQLTLSTNYNQGAKVSGPYVWDSSRNIYYILVDFTGTLIYPGGQDKYKKEVQFRIAAPQNVQWDNSNDYSFQDIKGVSSGSVVKTKYIPLYDEDIKVWGEEPQ",
    "response#2": "PTNDEFFVEAGINASGTNFIEIKAIVNNQSGWPARATNKLKFRYFVDLSELIKAGYSPNQLTLSTNYNQGAKVSGPYVWDSSRNIYYILVDFTGTLIYPGGQDKYKKEVQFRIAAPQNVQWDNSNDYSFQDIKGVSSGSVVKTKYIPLYDEDIKVWGEEPC",
    "response#3": "PTNDEFFVEAGINASGTNFIEIKAIVNNQSGWPARATNKLKFRYFVDLSELIKAGYSPNQLTLSTNYNQGAKVSGPYVWDSSRNIYYILVDFTGTLIYPGGQDKYKKEVQFRIAAPQNVQWDNSNDYSFQDIKGVSSGSVVKTKYIPLYDEDIKVWGEEPC"
  },
  {
    "reference": "MKPTLVFATANENKVSEVRRLLEDRFEVKSLVDIGCFEDIPETALTLAGNAKQKADYVKHKYGFDCFADDTGLEVHALNGAPGVFTARFGGPQKDAMQNMNHLLSELHSAGAKESMDRQAQFKTAIHLIVKGEVISLEGICKGEIAQKRSGTEGFGYDPIFIPEGETRTFSEMNADEKNAISHRGKAIRKMTEWLGV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must be able to regulate purine nucleoside triphosphate catabolic process. The designed protein sequence is ",
    "response#1": "SCPCTAGAA",
    "response#2": "MGIF",
    "response#3": "GRRRRF"
  },
  {
    "reference": "MDPNDASLTCAVNVEWNDPHGATMRKFAHKQTTLRLARNGLKEMFLEVTAEKQSPLRLKLKSVQVHSKFMAEGKASIKFNEEKCTLFLSNAPPGLLVQFLKVVFIKLTNGNGNSSGAKEPLSKTELLKKSRTQTLAGKSLNQFDEISPVTTNELIRARKLALGKGSTTTPSPSQKRKRPSDDPSSDRPKPKKLLSEETVDESAVIEGVQLNQEQKRILSACQSGQSVFFTGSAGTGKSFLLRKIIATLPPDGTIATASTGVAACLIGGTTLHSFAGIGTGEATLQNCYEKAARPNTAQAWRKCKRLIIDEISMIDADFFEKIEAVARYIRKNDKPFGGIQLILCGDFFQLPPIGRLPDRSRPGQYSQDAYQDDDSGRVRFCFESKVWQECIQASYELTIVHRQRDQEFITILNSIRIGRVTDEISERLRKTASQRIESEGILATQLCSHTSEVDAINQAKLEALRSEPKTFDAKDSDPYSIKQIDMMLQAPSKLTLKIGAQVMLLKNYNIAEGLVNGARGVIINFVQGLPLVKFKQRELLVRHEKWSVKTGSGMVLTRIQLPLKLAWAFSIHKSQGLTLDCVELSLSKVFEAGQAYVALSRAQSLDSIRVLDFDLRQVWANMKVLEFYRDLRKRINAMNNMEPVAVKRSLKKSLSSMGLTKSIMSKPLMTIK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The DNA binding domain within the designed protein should be capable of recognizing and binding to a range of DNA sequences.2. A protein that can perform ATP hydrolysis activity, 5'-3' DNA helicase activity, ATP binding in mitochondrion to support DNA repair, mitochondrial genome maintenance.3. The protein should be designed to have high stability and activity towards ATP + H2O = ADP + H(+) + phosphate. The designed protein sequence is ",
    "response#1": "HGTRALEINKMVSFWRNAHKRVSSQVVVWLKKGELPRAVTRHPAYSEEEDYGAILPQVVTAGTITRRAVEPTWLTASNARADRVGSELKAMVQVPPGYSLVGADVDSQELWIAAVLGEAHFAGMHGCTAFGWMTLQGKKSDGTDLHSKTAATVGISREHAKVFNYGRIYGAGQPFAERLLMQFNHRLTQQQAREKAQQMYAVTKGIRRFHLSEEGEWLVKELELAVDKAEDGTVSAQDVQKIQREAMRKSRRKKKWDVVAHRMWAGGTESEMFNKLESIALSASPQTPVLGCHISRALEPAVAKGEFLTSRVNWVVQSSAVDYLHLMLVSMKWLFEEYDINGRFCISIHDEVRYLVQEQDRYRAALALQITNLLTRCMFAYKLGLQDLPQSVAFFSAVDIDRCLRKEVTMNCATPSNS",
    "response#2": "RGYNTYIGGGAGTGKSLLLRVIRQELVSRGLTVATTATTGIAARRLNGATLHHCFGVNVYGEFTRRAELKEFDVIIIDEVSMLSKELFESLEFQLRRANGVDLPFGGVQVILSGDFLQLGAICSVSLVHSSVFRRNFAMLKLR",
    "response#3": "LSDEQKSVVRCVVNSRVNVFFTGSAGTGKSVILRRIIEMLPAGSTYITAATGVAASQIGGITLHAFCGFRYENSTAEQCLQQVLRHSHMVKQWKQCAYLIIDEISMIDCDFFEILEYVARAVRNNDRPFGGIQLIITGDFFQLPPVTKDNNPTFCFESEAWSRCVQKTIVLKNVKRQNDNVFVKILNSVRVGVCDR"
  },
  {
    "reference": "MHYITPDLCDAYPELVQVVEPMFANYGGRDSFGGQMVTIKCHEDNSLVKEQVDLPGEGRVLVVDGGGSLRRALLGDMLAEKAAKNGWAGIVVYGCIRDVDVIAQADLGVQALASHPMKTDKRGIGDLNVPVTFGGVTFKPGEYLYADNNGIIVSPQALSMPE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must be able to perform 4-hydroxy-4-methyl-2-oxoglutarate aldolase activity, ribonuclease inhibitor activity under specific conditions.2. For general function, the protein need meet that Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions .3. The substrate binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "IQGTAAVTLGTLIAASRAAGSQLSEQKIVFLGAGSAGCGIAEQIIAWMRTEGGLSDEQARARVFMVDRFGLLTDNMPNLLSFQSKLVQKRDSLKGWDTQSDSISLLDVVRNAKPDILIGVSGQTGLFTEEIIREMHKHCARPIVMPLSNPTSRVEATPHDILNWTDGAALVATGSPFQPVTVKEKTYPIAQCNNAYIFPGIGLGIISSGALRVTDEMMMAASEALASHSPLVNTGSGLVLPPLTDIQQVSKDIAFAVGKML",
    "response#2": "IQGTAAVTLGTLIAASRAAGSQLSEQKIVFLGAGSAGCGIAEQIIAWMRTEGGLSDEQARARVFMVDRFGLLTDNMPNLLSFQSKLVQKRDSLKGWDTQSDSISLLDVVRNAKPDILIGVSGQTGLFTEEIIREMHKHCARPIVMPLSNPTSRVEATPHDILNWTDGAALVATGSPFQPVTVKEKTYPIAQCNNAYIFPGIGLGIISSGALRVTDEMMMAASEALASHSPLVNTGSGLVLPPLTDIQQVSKDIAFAVGKML",
    "response#3": "IQGTAAVTLGTLIAASRAAGSQLSEQKIVFLGAGSAGCGIAEQIIAWMRTEGGLSDEQARARVFMVDRFGLLTDNMPNLLSFQSKLVQKRDSLKGWDTQSDSISLLDVVRNAKPDILIGVSGQTGLFTEEIIREMHKHCARPIVMPLSNPTSRVEATPHDILNWTDGAALVATGSPFQPVTVKEKTYPIAQCNNAYIFPGIGLGIISSGALRVTDEMMMAASEALASHSPLVNTGSGLVLPPLTDIQQVSKDIAFAVGKMV"
  },
  {
    "reference": "MLLGILVVLCFIPAADVTENKILVAQHPLLIVANKTANLVCNYSYNGTGKEFRASLHKGTNSAVEVCFISWNKTKSSSNSNKEFNCQGTFYEDKVIFNLWNMNANQTDIYFCKIEAMYPPPYVYNEKSNGTVIHVKETPVQTQEPQSAISYWVMVAVTGLLAFYSILITAIFISYWQKSKKNRYHQSDYMNMTPRHPPYQKNKGYPSYAPTRDYTAYRSWQP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be optimized for stability and expression in external side of plasma membrane where it will participate in positive regulation of interleukin-2 production, phosphatidylinositol 3-kinase signaling.2. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "SQPIQIDSSGKSGAQFYQSYDKFFIIKSLTSEEIERMHAFLKQYHPYVVERHGKTLLPQYLGMYRITVESVQYYFVVMRNVFSSHLTIHKKFDLKGSTVDREASEKELEKNLPTFKDNDFIKQKVKLDIGKEAKDKLMDTLSNDVDLLTKLHIMDYSLLVGVHDCVRAEEEALQGDNILTVGRSENSESEECDSGERWTYNTPPDSPRGAQYKEVVYEVDIYDIPSIEEKREIYFIAIIDVLTQYGVKKQAAKAAKTVKYGSNVDGISTCDPEQYAKRFLDFMDK",
    "response#2": "SQPIQIDSSGKSGAQFYQSYDKFFIIKSLTSEEIERMHAFLKQYHPYVVERHGKTLLPQYLGMYRITVESVQYYFVVMRNVFSSHLTIHKKFDLKGSTVDREASEKELEKNLPTFKDNDFIKQKVKLDIGKEAKDKLMDTLSNDVDLLTKLHIMDYSLLVGVHDCVRAEEEALQGDNILTVGRSENSESEECDSGERWTYNTPPDSPRGAQYKEVVYEVDIYDIPSIEEKREIYFIAIIDVLTQYGVKKQAAKAAKTVKYGSNVDGISTCDPEQYAKRFLDFMDC",
    "response#3": "EVNLSCKYTYNLFSKEFRASLYKGADSALEVCVVNGNHSHQLQFHSNTGFNCGGKLDNESVTFYLWDLNVNQTDIYFCKR"
  },
  {
    "reference": "MTDFRLALATGNKHKVRELFAILEPLLPGLTVDQIGTTHDFDVAEPVEDAPSFEGNALIKARALADATGLPVIADDSGLAVDILGGAPGIFSARWSGHHGDDEANLTLLLNQLADVPTQYRGAKFVCAAAMVTPGDGAVSAREIVEVGAMPGRLITEPRGDGGFGYDPIFVPDGYEVTSAELSPEEKNRISHRGKAFRALAPSIVEALRNV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be optimized for dITP diphosphatase activity, XTP diphosphatase activity activity in order to improve nucleotide metabolic process, purine nucleoside triphosphate catabolic process.2. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+) binding.3. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP The designed protein sequence is ",
    "response#1": "MPKQKIVLDCDPGHDDAVAILLALASPELDVRGITTTHGNTTLENTTRNALRVCELLGRADIPVRRGASRPLVREPLTAAEIHGESGLDGPDLPEPALEPEHASEYIVWLMREAARPGEVSLIALGPLTNLAVALHLYPDLPERIKRLVIMGGAFHTKGNITPVAEFNFWADAEAARIVFGAGWEITMVGLDVTHQALVTREMVERLRPLGSRAGKLAEMMIDFWLEYTRPVYGMEEAAIHDPLAVAVAIDPTLFTCQQAFVEVELTGTLSRGRTVVDRRQFQPGRPNVQVGLRVDAERFWNLFVERLSRLP",
    "response#2": "MSILSGKKIVFATHNPHKVEEIRALMGDNFEIVSLADIGCHEDIPETADTIEGNAYLKARYVHEHYGLDCFADDTGLEVEALNGAPGVYSARYAGEQKDAANNMALLLSKMKDKSSRSARFRTVISLIIGEKEYQFEGVVKGSITREPRGDKGFGYDPVFQPLGYDITFAEMSLEEKNSISHRGLALRKLVAFLKQS",
    "response#3": "MNIILLGPPGAGKGTQAKILAENYNIPHISSGDIFRTNIKENTDLGIKAKSYIDKGDLV"
  },
  {
    "reference": "MGCNRNCGLITGAVVGAVLAILGGVLMPVGDMIIQKTIKKQGIIEEGTVAYKSWEKTGTDVYRQFWIFDVQNPDEVRVNNSKIVVKQKGPYTYRVRFLPKENITKNSDNTISFMQPNAAIFEPSMSVGSEYDTCTVVNLAVVAVPVLFPNAFVQRLLNSYIKKSKSSLFQIRTVKELLWGYTDPFLQLVPYPVDTTVGVFYPYNNTADGEYKIFTGKDDISKVAIIDTYKDKRNLSYWPGYCDMINGTDAASFPPFLDKNRVLQFFSSDICRSIYAKFEREINLKGIPVYRYVLPRMAFASPVENPDNYCFCTEKVISRNCTSAGVLDISSCKQKKPVYISLPHFLHASLDVTDPVEGLSPNQEEHQTYLDVEPITGFTLAFAKRLQVNLLISPAPLIEPLSKLKRSYITPVLWLNETGTIGDEKAELFRAQVIGKINLLGLLQLVLLILGLATFVSFTIAYCACRTKSIK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. A protein that can perform lipoteichoic acid immune receptor activity, low-density lipoprotein particle receptor activity, scavenger receptor activity, low-density lipoprotein particle binding, short-chain fatty acid transmembrane transporter activity in receptor complex, Golgi apparatus, cytoplasm, cell surface, apical plasma membrane to support cellular response to lipoteichoic acid, energy homeostasis, negative regulation of protein import into nucleus, lipid transport across blood-brain barrier. The designed protein sequence is ",
    "response#1": "PLLRVLLVLLASGKGESYHVYDDVQKEKTTFKGKTRSCTRHSASALGQGRVAWSLSGCSSYSLPQSCLEPTTPQCSRAWRASPYRRRNGSTAITDDTLGGTLTITLRNLQPHDAGFYQCQSLHGSEADTLRKVLG",
    "response#2": "LESHHKGHFIYKNISEVLPLPAFSPTLLGDTRMLYFWFSEQVLDSLAKAAFQDGRLQLNLAETELKVVLETWHFNPNQEIIPEVITGFPSPGQVTVHCPRRPTISCQNKGVVVSSPVVMEFLFPHKDGQDSVTHTFEEDIVATIQASYSKKKLFLSLVDFQIKPKTTSNMAESSESIQNFLQLMITTVGIPEIMSRLEVALTTLMNSKGLDLFDIINPEIITRDGFLLLQMDR",
    "response#3": "LESHHKGHFIYKNISEVLPLPAFSPTLLGDTRMLYFWFSEQVLDSLAKAAFQDGRLQLNLAETELKVVLETWHFNPNQEIIPEVITGFPSPGQVTVHCPRRPTISCQNKGVVVSSPVVMEFLFPHKDGQDSVTHTFEEDIVATIQASYSKKKLFLSLVDFQIKPKTTSNMAESSESIQNFLQLMITTVGIPEIMSRLEVALTTLMNSKGLDLFDIINPEIITRDGFLLLQMDEP"
  },
  {
    "reference": "MKRKERRINKDFGYNRKCVCHYEASQKRFCYSQYSCASVLYERVRDIAKIMDRLDSGLDAWCLRDAIISVLRATHCVPRVDRMLGRWYLKTSIFYDFCPDDLILSCPNVIMPNVLNFVKKYRDFIRSVLYKVSVSWKNQYMPGVLGASRFLEEISNSLNGVEESIPCIYLRMCATLTEIVLRNGYLREIYQENPYVIFEELAFSLFTQKWVLPFSCMTNLGLVEKANSTVFDVAIYNTCLYSLADFITVNGEHLFPALLNGSNISMNVTRYQQEAKNIFEILLSQIQVVERDTDKTVQLTVYVEVWHVSALTWLDLYQVLPETSRVTFCLIIPGIFMDRYELKRAQWSLFHKNIAFELGKCDEVTFSTKYLEFERTTDHAKITMASFVEKICRCLKRGRMGLIFRKNVYQYSMIPHVPLYCGGDFLDVLPVRDGINTCLRMLLNVVHFLGDEVSDELTEEIDFVRLQCKFFMFNELRRVVRKMVLVANAVIDYAVDNKDFLREGIVDGRSLGICITGLHSVFMTVGLSYAHPDACRLYRMMCEHIYYTCVRTSVDCCMKGAEPCNLFDRSKYALGMLYFDQFDNVECTLPEELWTTLRKDVLMHGVRNIHFTAGTAMQKEFDIINSSESFWPMEDNKILRRSNIKVVIGKDGLNDVTSVYSSELKSLYIPVYNNLLLNRFNKHQQYLKTVGYRVLNVDTNLFTDKELDDLAVFKDGFSYPLNDLIEMYKSGLPFLDQGQANVFYFNDTVSLKHLLPLLYKTGFKVAMY",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in virion component that is critical for DNA replication. The designed protein sequence is ",
    "response#1": "ISLRKVIFEGQQIRIYTCLLEEAAARQMVVPTFRSGAQRGAAPGAGGGGGEETTYQGATVFEPTVGYHHAPVAVFDFASLYPSIIMAHNLCYSTWLRDY",
    "response#2": "APRVLNAIKSGATSLDLHSISPHFYTLAEKWATLFSDKELSQVLKDMLLERGQEINNHAISVSMFSNCNQTNDSAVFLQTLDEFEKTIYRDTNVNCKEMKKWM",
    "response#3": "GMEAARALMDKYHVDNDLKANFKLLPDQVEALAAVCKTWLNEEHRGLQLTFTSKKTFVTMMGRFLQAYLQSFAEVTYKC"
  },
  {
    "reference": "MLKFTAEHEWLELDGDVATVGITTYAVEQLGDLVFVELPEVGKSFSKNDDAATVESVKAASEVYCPLDGEITAVNDAIVADPSLINSDPQGAGWFFKLKLKNRADADGLLDEAAYKELIA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be expressed specifically in the glycine cleavage complex The designed protein sequence is ",
    "response#1": "TIVVGITDLAQTMAGKIVKVRIKKKGTKVEKGRPVATMESGKWAGPVPAPVTGEVVEVNAEAEKSPIIINQDPYGKGWLVKML",
    "response#2": "TIVVGITDLAQTMAGKIVKVRIKKKGTKVEKGRPVATMESGKWAGPVPAPVTGEVVEVNAEAEKSPIIINQDPYGKGWLVKMK",
    "response#3": "HAVVGITDFAQESLGDLMDVHLPEVGADIDQGEEIMSLESVKAASDIFSPLSGEVVAVNTELEDEPERVNDEPYDGGWLFKLK"
  },
  {
    "reference": "MTVPVITIDGPSASGKGTVAAQVAARLAFHYLDSGSLYRLLALHARRRGIAWDDEPALARAAAALPVEFSDGAVWLEGNDASQDIRAEEIGIGASKVGALPAVRAALLQRQRDFCEAPGLVTDGRDMGSVVFPDATLKIFLTASAEERASRRHKQLIGKGESANLAQIMQDIIDRDARDAARPVAPLRQEPDAFLLDTTELTIDQAVDKVLEWFEEKQASGH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. We are looking for a protein with broad substrate specificity for ATP + dCMP = ADP + dCDP.2. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.3. The protein design must be able to perform CMP kinase activity, dCMP kinase activity in order to contribute to pyrimidine nucleotide metabolic process, phosphorylation. The designed protein sequence is ",
    "response#1": "VIAVSGQPGSGKTTIAREIARVLGLPLVSSGLLFREMAARMGMDFIEFHKYAETNPDIDKKVDSLAIERAKAGDVVLEGHLTAWIVRPYADVCIYLKASLETRARRVALRDGKSLQDALREVAEREELNRRRYLSIYGIDINDLSIFDLVLDTR",
    "response#2": "VIAVSGQPGSGKTTIAREIARVLGLPLVSSGLLFREMAARMGMDFIEFHKYAETNPDIDKKVDSLAIERAKAGDVVLEGHLTAWIVRPYADVCIYLKASLETRARRVALRDGKSLQDALREVAEREELNRRRYLSIYGIDINDLSIFDLVLDTW",
    "response#3": "VITLDGPSASGKGTIARLVSQALGFHYLDSGALYRLVALAAMKRGVDAGDEQGVVGIARSLDVSFADSSIRLDGIDVSDEIRAEACGEYASKIAQYSALRVELLERQRDLRKLPGLVADGRDMGSVIFPDATLKIYLTANEEERARRRHKQLMEKGINASIAHLVLALRERDERDSKRVASPLQQCEDTRLLDTSGLTMDAVKAIVL"
  },
  {
    "reference": "MTAPELRAPAGHPQEPPARSSPAQALSSYHHFPTSDQERWYQETGSLCSRFLEAGQYGLHQQYQFMFFFMHHLIPALGPYPQKWRSTISRSGLPIEFSLNFQKGSHRLLRIGFEPVNFLSGSSQDPFNRIPIADLLAQLARLQLRGFDTQCFQQLLTRFQLSLDEVRQLPPDDQPLKSQGAFGFDFNPDGAILVKGYVFPYLKAKAAGVPVATLIAESVRAIDADRNQFMHAFSLINDYMQESTGYNEYTFLSCDLVEMSRQRVKIYGAHTEVTWAKIAEMWTLGGRLIEEPEIMEGLARLKQIWSLLQIGEGSRAFKGGFDYGKASATDQIPSPIIWNYEISPGSSFPVPKFYLPVHGENDLRVARSLAQFWDSLGWSEHACAYPDMLQQLYPDLDVSRTSRLQSWISYSYTAKKGVYMSVYFHSQSTYLWEED",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have prenyltransferase activity to facilitate alkaloid metabolic process.2. For general function, the protein need meet that Deoxybrevianamide E synthase; part of the gene cluster that mediates the biosynthesis of notoamide, a fungal indole alkaloid that belongs to a family of natural products containing a characteristic bicyclo[2.2.2]diazaoctane core; This step would be followed by an indole 2,3-epoxidation-initiated pinacol-like rearrangement catalyzed by the notB' FAD-dependent monooxygenase leading to the formation of notoamide C and notoamide D (Probable); The reverse prenyltransferase notF' then acts as a deoxybrevianamide E synthase and converts brevianamide F to deoxybrevianamide E via reverse prenylation at C-2 of the indole ring leading to the bicyclo[2.2.2]diazaoctane core3. The designed protein should have a high specificity for brevianamide F, dimethylallyl diphosphate binding, with minimal off-target effects.4. The protein design should consider the endogenous proteins and enzymes present in the Alkaloid biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "TYSELDELSNRLASSLILLGVKAETFVPICMEKSRWATVAILGVMKAGGAFTLLDPSYPLPRLKTICEELSSLVVLSSTTQSERCTQLANVIVVEHLCRAWHPGAYLAQSPATVCPSNVLYVAFTSGSTGKPKGVLIEHRAYSTGAREHLRVFQIDQTSRVLQFSSYAFDVNIMETLSTLMAGGCLCVVGEAQRSDVSLFAEAVDELQVSHAMLTPSFARTVPWENVRHLRVLILGGEEMRESDAAICAERGVRLINAYGAAECSVNATARPGVQPGDNLSTIGHGTGAIAWIIDPDDPERQMGPGTAGELLLEGPIVGRGYLNSPDMTDRVFIDPPTWLRQLRMMDYQHQLYRTGDLAVQDSTGALTLLGRKEGQVKIRGQRVEVAEIEQHIDQVLTQEELEAGRHDYTYSELDELSNRLASSLILLGVKAETFVPICMEKSRWATVAILGVMKAGGAFTLLDPSYPLPRLKTICEELSSLVVLSSTTQSERCTQLANVIVVEHLCRAWHPGAYLAQSPATVCPSNVLYVAFTSGSTGKPKGVLIEHRAYSTGAREHLRVFQIDQTSRVLQFSSYAFDVNIMETLSTLMAGGCLCVVGEAQRSDVSLFAEAVDELQVSHAMLTPSFARTVPWENVRHLRVLILGGEEMRESDAAICAERGVRLINAYGAAECSVNATARPGVQPGDNLSTIGHGTGAIAWIIDPDDPERQMGPGTAGELLLEGPIVGRGYLNSPDMTDRVFIDPPTWLRQLRMMDYQHQLYRTGDLAVQDSTGALTLLGRKEGQVKIRGQRVEVAEIEQHIDQVLT",
    "response#2": "SQGRIPRYSAMVETTEQAQKAIMFARERQLRLVIKNTGHDSGGRSSAVDSFQILTQRLKDITFIPEFTPTLGLAEGTYKSKGPSVRIGAGVLTKELYAAADEHGYTVMGGECATVGIAGGYIQGGGVSTALTPMLGLAVDLVQEFEVITAEGRHVIANEFQNQDLFWALRGGGGGTFGLVTSVTMPVFGTTYSELDELSNRLASSLILLGVKAETFVPICMEKSRWATVAILGVMKAGGAFTLLDPSYPLPRLKTICEELSSLVVLSSTTQSERCTQLANVIVVEHLCRAWHPGAYLAQSPATVCPSNVLYVAFTSGSTGKPKGVLIEHRAYSTGAREHLRVFQIDQTSRVLQFSSYAFDVNIMETLSTLMAGGCLCVVGEAQRSDVSLFAEAVDELQVSHAMLTPSFARTVPWENVRHLRVLILGGEEMRESDAAICAERGVRLINAYGAAECSVNATARPGVQPGDNLSTIGHGTGAIAWIIDPDDPERQMGPGTAGELLLEGPIVGRGYLNSPDMTDRVFIDPPTWLRQLRMMDYQHQLYRTGDLAVQDSTGALTLLGRKEGQVKIRGQRVEVAEIEQHIDQVLTE",
    "response#3": "QQRCREAPESSAVAAWDGSFTYSELDELSNRLASSLILLGVKAETFVPICMEKSRWATVAILGVMKAGGAFTLLDPSYPLPRLKTICEELSSLVVLSSTTQSERCTQLANVIVVEHLCRAWHPGAYLAQSPATVCPSNVLYVAFTSGSTGKPKGVLIEHRAYSTGAREHLRVFQIDQTSRVLQFSSYAFDVNIMETLSTLMAGGCLCVVGEAQRSDVSLFAEAVDELQVSHAMLTPSFARTVPWENVRHLRVLILGGEEMRESDAAICAERGVRLINAYGAAECSVNATARPGVQPGDNLSTIGHGTGAIAWIIDPDDPERQMGPGTAGELLLEGPIVGRGYLNDADKSSNAFFSHPAWSQGRIPRYSAMVETTEQAQKAIMFARERQLRLVIKNTGHDSGGRSSAVDSFQILTQRLKDITFIPEFTPTLGLAEGTYKSKGPSVRIGAGVLTKELYAAADEHGYTVMGGECATVGIAGGYIQGGGVSTALTPMLGLAVDLVQEFEVITAEGRHVIANEFQNQDLFWALRGGGGGTFGLVTSVTMPVFGSYIHDTNSQNPSPPTTATEHALQQLYADVLELPIMRISMEDSFVRLGGDSIMAVRLVGAARQAGLVLDIRDVLGTARLEEQARKATPVLPVDLPSYLNEADADLENWQEWFWGEKYNRLRDIKRKWDPDDLFLVRHGQ"
  },
  {
    "reference": "MYAPLALYDTAAARTLDAQATTLLGGDGYTLMQRAGQAAWQWLLERWPQARRIVVVCGTGNNGGDGYVLARLAQRAGCQVCVVHLPEHGPASALAQRACTDYLAVGGAIELFPTPLAQADVIVDALFGIGLNRAPDTQTAALIEAINAAGTPVLALDVPSGIDADHGVAFETAVRAEITLQFIVPHLGLYTGDALEYTGERGIAALDVPVSAFDGLHPAAHSWDIDALSAHLRPRRRNTHKGESGRVLCVGGNLGSGGAILLTAEAALRSGAGLVQVATRAEHVTALLARCPEAMVRAVQAGDDIAALADAADVVALGPGLGQDDWARALWQGVLGSDSAVVIDADGLNLLAATAAPPRGPRVLTPHPGEAGRLLGIPTAQVQRDRLAAAAALAQRFDAVVVLKGAGTVVAAPHAVPRIVDAGNPGMAVGGMGDLLTGVIASLLGQGWSAFDAASLGALLHGCAGDAAARDGERGLLPTDLLPQLRRLANAGASA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The AMP binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "GVAAGSAQYPGAAHMVVGAARHCGIGMVRLHAEPGQREAADAVVVRFPDVVVTEAAPKKDGRATGWTVGPGLGTDESAARVLESALRSDLPVVVDADGLTLLADHDELRSLVRSRRAPSVLTPHVGEFARLGFELGLDRIAATKIAAVESGAVIVLKGSSTVISDESGRVFVDMMGSPALATAGTGDALSGIVGAAICRAGEKASVETVAAAVYLHGLSGRIL",
    "response#2": "PWWRENVHEEFPEVRQWAVDVLDEEDELQEIVQLVGKDALPEDQQLTLEVARYIREAWLQQNAFHDVDTYCEPEKTYQMLTAIQHFNDEAFEALDAGVPVEES",
    "response#3": "GVAAGSAQYPGAAHMVVGAARHCGIGMVRLHAEPGQREAADAVVVRFPDVVVTEAAPKKDGRATGWTVGPGLGTDESAARVLESALRSDLPVVVDADGLTLLADHDELRSLVRSRRAPSVLTPHVGEFARLGFELGLDRIAATKIAAVESGAVIVLKGSSTVISDESGRVFVDMMGSPALATAGTGDALSGIVGAAICRAGEKASVETVAAAVYLHGLSGRIL"
  },
  {
    "reference": "MSNPVTNRCIVLGVTGSIAAYKAGEVASRLTQWGAKVDVVLTEGAQHFITPLTFQSLTGRKAYTDADLWGGEGHVTHVGLGRSADLIVIAPATANTLSKLAYGIADNLLVLTVLASQCPLVVAPAMDAGMFQHPATQAAVRILEERGAYFVGPAEGRLASGLVGRGRMVEAAEVLAAVRWLSGRNGPLAKRKVVITAGGTQEPIDAVRVITNRSSGKQGYALAQAALDVGADVVLITAPTSLIPPYGAKVIKVRTAAEMLDAVLQETQDADVLIMAAAVADFRPRVAQSQKIKKEREIPSLELEATPDILVEVAERRAHLGYPRRVIGFAAESEDLLANAQSKLQRKRLDMIVANDISAQDAGFEVDTNRVTLLFADGRMETLPLMEKDKVAEAVIGHLITMLI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should consider the endogenous proteins and enzymes present in the CoA from (R)- pantothenate: step 3/5 pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MRIILGVSAGIAAYKAAILLRLLREAGHAVRVVPTESSYAFVGRATWEALSGEPATHEVFDAVPEVAHVRLGQGADLVIVAPATADVLAKAATGRADDLLTTTLLTVRCPVLVAPAMHTEMWLHAATQANVATLRARGVHVLEPATGRLTGADSGPGRLPEPQEIAAAAVAAALDALG",
    "response#2": "MRIVLGVSAGIAAYKAVILARLLTEDGRQLEVIPTPAALEFVGRATWEAITGRPVTTSVFEGVDEVRHVRLGQQADLIVVAPATADLLARTRAGTAGDLLSSTLLASEAPKLLVPAMHTEMWRNPATVENVEVLRGRGIEVMEPASGRLTGTDSGPGRLPEPEQIKARIDQAVA",
    "response#3": "GSHLIVTAGPTHEPIDPVRYLANRSSGKQGFAIAGALAALGARVTLVAGPVTLATPDGVTRVDVETALEMAAAVEAALPADGAVLVAAVADWRVAAAAQKLKKQGGAPALQLVENPDILAGLARHERRPRLVVGFAAETERVIEHAQAKLARKGADWIVANDVSGTVMGGEANAVHLVDAEGHITGLEKMTKADRVALLKRVLLIVGGGIAAYKACTLVRLLKKSGIAVRCVLTEAGAQFVTPMTLAALSENPVHTSLWDLKDETEIGHIQLSREADLVAVVPASADLLAKMAAGIANDLATTLLLATDKPVLAAPAMNVRMWHHPATQRNLKQLRADGVTLLEPDEGPMACGEFGPGRLPEPEAIHAAILALAT"
  },
  {
    "reference": "MRHNNSGRRLGRTTSHRIAMFRNMVTSLLNHERIVTTDAKAKEIRSVAEKMITLGKRGDLHAHRQAAAYIREKSVVTKLFSTIAPRYKDRAGGYTRIIKLGQRLGDAASLSVIELVEEAAPQQS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that contributes to translation by functioning in ribonucleoprotein complex, ribosome with structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "LESRLDNLVYRLGLARTRRQARQLG",
    "response#2": "MLRRLDAVVYRAKFVSTMFAARQFINHGHIKVNGKRVNIPSYKVRVGDVIEVKEASKQLAFVLEASS",
    "response#3": "MFPNL"
  },
  {
    "reference": "MSSASNNGPSADQSVQDLQMEVERLTRELDQVSSASAQSAQYGLSLLEEKSALQQKCEELETLYDNTRHELDITQEALTKFQTSQKVTNKTGIEQEDALLNESAARETSLNLQIFDLENELKQLRHELERVRNERDRMLQENSDFGRDKSDSEADRLRLKSELKDLKFRETRMLSEYSELEEENISLQKQVSSLRSSQVEFEGAKHEIRRLTEEVELLNQQVDELANLKKIAEKQMEEALETLQGEREAKYALKKELDGHLNRESMYHISNLAYSIRSNMEDNASNNSDGEEENLALKRLEADLSTELKSPDGTKCDLFSEIHLNELKKLEKQLESMESEKTHLTANLREAQTSLDKSQNELQNFMSRLALLAAHVDALVQLKKQIDVKEQGKEGGQKKDELEQQLRALISQYANWFTLSAKEIDGLKTDIAELQKGLNYTDATTTLRNEVTNLKNKLLATEQKSLDLQSDVQTLTHISQNAGQSLGSARSTLVALSDDLAQLYHLVCTVNGETPTRVLLDHKTDDMSFENDSLTAIQSQFKSDVFIAKPQIVEDLQGLADSVEIKKYVDTVSDQIKYLKTAVEHTIDMNKHKIRSEGGDALEKVNTEEMEELQEQIVKLKSLLSVKREQIGTLRNVLKSNKQTAEVALTNLKSKYENEKIIVSDTMSKLRNELRLLKEDAATFSSLRAMFAARCEEYVTQVDDLNRQLEAAEEEKKTLNQLLRLAVQQKLALTQRLEEMEMDREMRHVRRPMPAQRGTSGKSSFSTR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should exhibit Polar residues compositional bias.2. The protein must exhibit the following characteristics: Plays a role in the biogenesis of annulate lamellae containing nuclear pore complex components.; This protein is essential for differentiation.3. The designed protein should have dynactin binding, clathrin heavy chain binding, cytoskeletal anchor activity within Golgi apparatus, cytoskeleton, cytosol, presynapse to regulate cellular macromolecule localization, oocyte nucleus migration involved in oocyte dorsal/ventral axis specification.4. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "CGPSSFHFSINLLSQDTATPPALVVWDRRGRLHKLQNDSGCGTWVHKGPGSSMGVEASYRGCYVTEWDSHYLMPIGLEEADAGGHRTVTETKLFKCR",
    "response#2": "CGPSSFHFSINLLSQDTATPPALVVWDRRGRLHKLQNDSGCGTWVHKGPGSSMGVEASYRGCYVTEWDSHYLMPIGLEEADAGGHRTVTETKLFKCS",
    "response#3": "CGPSSFHFSINLLSQDTATPPALVVWDRRGRLHKLQNDSGCGTWVHKGPGSSMGVEASYRGCYVTEWDSHYLMPIGLEEADAGGHRTVTETKLFKCS"
  },
  {
    "reference": "MKSKGTRFERDLLVELWKAGFAAIRVAGSGVSPFPCPDIVAGNGRTYLAIEVKMRKELPLYLSADEVEQLVTFARGFGAEAYVALKLPRKKWRFFPVQMLERTEKNFKIDESVYPLGLEIAEVAGKFFQERFGEKV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The design objective is to achieve a protein with helix-strand-strand-strand-strand-strand-strand-strand-helix-strand-strand-helix-strand-strand-turn characteristics.2. The protein must exhibit the following characteristics: A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination; Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products..3. The designed protein must possess crossover junction endodeoxyribonuclease activity, magnesium ion binding, DNA binding to support DNA recombination, DNA repair under certain conditions.4. The protein requires Mg(2+) to activate a critical catalytic site.5. The design of the protein must focus on the precise arrangement of amino acids in the active site to enable effective catalysis of Endonucleolytic cleavage at a junction such as a reciprocal   single-stranded crossover between two homologous DNA duplexes   (Holliday junction).. The designed protein sequence is ",
    "response#1": "MAPLEAAPPAPPSRRGPAPAPAPAPPPPPPPSPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPA",
    "response#2": "MALVKLAQYPAIQALSDAQAAFEAVRLAFKQDQLPLDYQVGVHVCQEFLLKGASSKPTLESWLEVVGASSTELLGMLRKLRKKHPQYNNKPMPMGPMVPMPMGMPGMSNMGGMPNMSNMGGMPNMSNMGGMPNMGGMPNMGGMPNMGGMPNMGGMPNMGGMPNMGGMPNMGGMNNMGGMPNMGGMPNMGGMPNMGGMPNMGGMPNMGGMPNMGGMPNMGGMPNMGGMPNMGGMPNMGGMPNMGGMPNMGGMPNMGGMPNMGGMPNMGGMPNMGGMPNMG",
    "response#3": "MCCYAMPYCTVPCCTSVCCTPVCCRPICSGCCDPCCCQPCCRPTCCQNTCCGSSCCQPCCGPSCSQTCCQPCCRPTCCQNTCCGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSCCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCSGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCSGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRCQPCCGGSSRC"
  },
  {
    "reference": "MGGKWSKRSXGGWPAVRERMKQAEPAAEGVGAASRDLEKXGAITSSNTAXTNAACAWLEAQEEEEVGFPVRPQVPLRPMTYKGAXDLSHFLKEKGGLEGLIXSQKRQDILDLWXYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPEQVEKANEGENNXLLHPMSLHGMXDTEXEVLMWKFDSRLAFHHMARELHPEYYKDC",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be optimized for SH3 domain binding.2. The protein must exhibit the following characteristics: Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1. The designed protein sequence is ",
    "response#1": "MPQLDTSTWFTILITSLIFITLMIIH",
    "response#2": "MPS",
    "response#3": "MIPRL"
  },
  {
    "reference": "MVVKIVEAYEKVDQVKILFKEYVEWLNIDLSFQNFSEEFNSLPGKYSIENDGRLYIAYSDESLAGCVGLRKINIEINENKNNQQVDHDETSTIIIKKEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQKICEMKRLFVKSEFRGLKIGKLLVERLINDAKEQNYDYMVLDTLKTLTTAISIYKSFGFIEFDPYYYNPNSNDVTFLKLKLK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must have high specificity towards acyltransferase activity, transferring groups other than amino-acyl groups.2. The designed protein have N-acetyltransferase domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "LDINYKTDELFEDFRNFGNKDLYLVDELRGEMIDASSDSPFYGIYVGDCLGARMALYRKGEVEETHFPGFDDYNVIWKLEVLRDFQGRGYGTSLLDFAKDQGLPIKVIARNQSKDFFIKQGFTDLEETNRDGHDVLVWTR",
    "response#2": "LEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSDSPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELIDFAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKL",
    "response#3": "TMMEDLENNIIENDSKSPFYGIYFGNNLIARMSLYEVSKQYDYYFEPSQDYLTLWKLEVLPDYQNKGYGEVLVNFAKSFELPIKTNPRINSHGFWEKMGD"
  },
  {
    "reference": "MSVWRPSEATVYLPPVPVSKVVSTDEYVSRTSIYYYAGSSRLLAVGHPYFSIKNPTNAKKLLVPKVSGLQYRVFRVRLPDPNKFGFPDTSFYNPDTQRLVWACVGLEIGRGQPLGVGISGHPLLNKFDDTETGNKYPGQPGADNRECLSMDYKQTQLCLLGCKPPTGEHWGKGVACTNAAPANDCPPLELINTIIEDGDMVDTGFGCMDFKTLQANKSDVPIDICGSTCKYPDYLKMTSEPYGDSLFFFLRREQMFVRHFFNRAGTLGEAVPDDLYIKGSGTTASIQSSAFFPTPSGSMVTSESQLFNKPYWLQRAQGHNNGICWGNQVFVTVVDTTRSTNMTLCTQVTSDSTYKNENFKEYIRHVEEYDLQFVFQLCKVTLTAEVMTYIHAMNPDILEDWQFGLTPPPSASLQDTYRFVTSQAITCQKTVPPKEKEDPLGKYTFWEVDLKEKFSADLDQFPLGRKFLLQAGLKAKPKLKRAAPTSTRTSSAKRKKVKK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins.2. The designed protein should be expressed specifically in the T=7 icosahedral viral capsid3. The target protein must exhibit helix-strand-strand-strand-strand-helix-turn-strand-strand-strand-strand-strand-strand-strand-strand as its primary conformation. The designed protein sequence is ",
    "response#1": "LSRVLSIPNYWDRFTWKTSDVTNTVLWDNYVSPFKVKPYSATITDRFRCTHMGYVANAFTYWRGSIVYTFKFVKTQYHSGRLRISFIPYYYNTTISTGTPDVSRTQKIVVDLRTSTEVSFTVPYIASRPWLYCIRPESSWLSKDNKDGALMYNCVSGIVRVEVLNQLVAAQNVFSEIDVICEVSGGPDLEFAGPTCPA",
    "response#2": "SMAAPVAISRIVRGSKPRFIRSKGSVTITHRELVGQFNSSSALVVNGGITGNLYKINPANAVLFPWLQTLASNFDQYMFNTLRLQYVPMCASTETGRVAIYFDKDSQDLEPVDRIELANMRHLTETAPWCEGSLRVPVDSVKRFMNDNSTVDPKLIDLGQVGLATYGGPGTNAVGDLFIHYTVTFYEPQR",
    "response#3": "SMAAPVAISRIVRGSKPRFIRSKGSVTITHRELVGQFNSSSALVVNGGITGNLYKINPANAVLFPWLQTLASNFDQYMFNTLRLQYVPMCASTETGRVAIYFDKDSQDLEPVDRIELANMRHLTETAPWCEGSLRVPVDSVKRFMNDNSTVDPKLIDLGQVGLATYGGPGTNAVGDLFIHYTVTFYEPQR"
  },
  {
    "reference": "MSDFPGTEELESLDAYWRAANYLTVGQIYLQGNALLTRPLTLADVKPRLLGHWGTSPGLNLVYTHLNRLIHQYSLNVLFVTGPGHGGPALVAQAYLDGTYTELNPSVERNALGMARLFRQFSWPYGIGSHVGPHVPGSIHEGGELGYSLAHAYGAALDNPQLIVACVIGDGEAETGPLAASWHCNKFLNPARDGAVLPILHLNGYKIANPSVLSMISEEELTSLMYGYGYDPYFVEGDEPMEVHRALARTLDVIYEKISQIQRLARYAEDPQVVDRPLWPVLILRTPKGWTGPRYVDGQRVEGTWRSHQLPLADLDKPPHLMQLQQWLESYRPHELFDAQGTLLPELAALTPTGRRRMSANPHANGGLLLRALDLPHYADYCVPMPGPGQVRAEATRVLGGYVRDVMKNSLASQNFRLFGPDETASNRLDAVFEVTDKVWLDARASDDRHLSAQGRVMEILSEHICEGWLEGYLLTGRHGLFSCYEAFIHIVDSMINQHAKWLKTAAEVPWRKPIASLNILLTSHVWRQDHNGFSHQDPGLLDLLANKKANLARIYLPPDANCLLSVAEHCLRSRGYINVIVAGKQMEWQWLDCEAAAVHCQSGIGRWGWACHDDEMPEVVLACAGDVPTLEVLAAVTLLQEQAPDVRVRVVNVVDLMALQLSQQHPHGLSDSDFNGLFTEDRPVIFAFHGYPALIHRLIYKRANPQRFHVRGFREEGATTTPFDMAVINNLDRYQLLLDVFERVPRLQPQLEQARARYWATMEKHKL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein requires thiamine diphosphate to activate a critical catalytic site.2. The designed protein should have aldehyde-lyase activity to facilitate carbohydrate metabolic process. The designed protein sequence is ",
    "response#1": "LRSVRKGSGKQQIATTMATVRTFKELMRDKGLADRLVPIIPDEARTFGLDSWFPTLKIYNPHGQNYVPVDHDLMLSYREAPEGQILHEGINEAGSVASFIAAGTSYATHGKAMIPLYIFYSMFGFQRTGDSIWAAADQMARGFLLGATAGRTTLTGEGLQHMDGHSPVLASTNEGVETYDPSFAYEIAHLVHRGIDRMYGPGKGEDVIYYITIYNEP",
    "response#2": "KQPALAYLDMDAAIAHCTRGLGIWDWASTARSIGAEPDVVLACAGDIPTLETLAAADILRRELPDLAVRVVNVVDLMRLQPDSEHPHGLPDREFDALFTRDRPVIFAYHGYPWLIHRLTYRRANHAQLHVRGFKERGTTTTPFDMVMLNDLDRFHLVIDVLDRVEGLASRAAMLRQRR",
    "response#3": "MRRMVYTRVLDQRSISLNRQGRLGFYAPTAGQEASQLASHFALEAEDFILPGYRDVPQLVWHGLPLYQAFLFSRGHFMGNQMPENVNALAPQIIIGAQIIQTAGVALGMKLRGKKSVAITYTGDGGASQGDFYEGMNFAGAFKAPAIFVVQNNRYAISTPVEKQSAAKTVAQKAVAAGIYGIQVDGMDPLAVYAATAFARERAVNGEGPTLIETLTFRYGPHTMAGDDPTRYRTKDIENEWEQKDPIVRFRAFLENKGLWSQEVEEKVIEEAKEDIKQAIAKADQAPKQKK"
  },
  {
    "reference": "MELLFATANVHKCHEAATILGPSVSIIMPSSLGFSGEIPETGLNLEENALQKALFIWDIFHIPCFADDTGLEVDALNGEPGVYSARYAGPACSPRANVKKLLKEMEGLACRKARFRCVIALIREGRPVLYEGIVPGSILTAPRGNGGFGYDPVFVPLGFNQTFAQMTEADKNAISHRKRALEKMALAFP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..2. A protein with ribonucleoside triphosphate phosphatase activity, dITP diphosphatase activity is needed to enhance purine nucleoside triphosphate catabolic process, nucleotide metabolic process in a specific context.3. The protein requires Mg(2+) to activate a critical catalytic site.4. The Proton acceptor of the protein must be engineered to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MILATHNPGKVREIAA",
    "response#2": "MEIILATHNQGKVNELRAILSDFG",
    "response#3": "MAPDRYFMTAEEV"
  },
  {
    "reference": "MSAPSSPRVTRIAPSPTGDPHVGTAYIGLFNHTLARQSGGRFILRVEDTDRNRYVPDSEKRIFQMMQWLNLTPDESPLQGGPNGPYRQSERFDLYGDYARQLVQSGHAYYAFETSDELAALREEAQKAGHVIAIPSRDLGAAQAQARVDAGEPAVIRLKVDRDGETVVNDLLRDPIHFANKEIDDKVLLKADGFPTYHLANVVDDRLMQVTHVVRAEEWITSTPIHVLLYRAFGWPEPVFAHMPLLRNADKSKISKRKNPTSVEWYQNQGFLPEAMLNFLATMGWTHPDGQEIFDLAEFERVFRLEDVTLGGPVFDLAKLRWYNGKYLREVLSEDDVARRLHAFLMQNKVTLPSVDGPQDPYFRAVTHLMIPRLEVFADFMDKTLYFWSEDYPVNEKAQKAIDAGKELLPELAARLKNLPTFDAASIKEMFHAYAEEKGLKMGKVMPPIRAAVAGTMESPDLPEMLEALGRERVLARVEKAAR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design must be focused on enhancing the efficiency of glutamyl-tRNA aminoacylation.2. The protein have enhanced ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-   glutamyl-tRNA(Glu).3. The protein design should include a 'KMSKS' region motif to ensure proper function. The designed protein sequence is ",
    "response#1": "VKKGYLLKGLSELKKRTKNLVNLAKESLFYVEDIPISIDQESITVIKDYKHIFSILYDNLSKISEKDWNHNILMSIIKDISCNLEVKISIVYHCLRASITGRMNAPSIIEIMINLQHKECLQRIKYAE",
    "response#2": "LAEKARPHVEAAGYDVSDDRLQTICGLVQDRIQVVPEVVTDNRYFFEDPEAYEEAGVEKRWNEESADLLLAYADRLEGVDAFDTDTVETELRDLADEKDVGAGAIIHPSRLAVSGRSYGPGVFGLLAAVGKEACIRRMHRVVTRFAPSPTGFLHIGGARTALFNWLYARGRGGKFLLRIEDTDRARSTPEATAAILQGMAWLGLDHDGEVISQFERAPRHAEVALELLAQGKAYKCFSTQDEIQAFREAAQAEKRSTLFRSPWRDADPATHPDAPYVIRIKAPQSGETVIRDQVQGDVTIRNDQLDDMVLLRSDGTPVYMLAVVVDDHDMGVTHVIRGDDHLNNAARQMMIYQAMGWEVPVWAHIPLIHGPDGKKLSKRHGALGAQEYQAMGYPAAGMRNYLARLGWSHGDDEFFTDAQAREWFDLDGIGKSPARFDLKKLENLSGQHR",
    "response#3": "LAEKARPHVEAAGYDVSDDRLQTICGLVQDRIQVVPEVVTDNRYFFEDPEAYEEAGVEKRWNEESADLLLAYADRLEGVDAFDTDTVETELRDLADEKDVGAGAIIHPSRLAVSGRSYGPGVFGLLAAVGKEACIRRMHRN"
  },
  {
    "reference": "MNAGPLPEHLRRVHMVGIGGAGMSGIARILLDRGAQVSGSDAKESRGVLALRARGALVNIGHDGDALDLLPGGPTVVVTTHAAIPKTNPELVEANRRGIPVLLRPVVLADLMAGYRTLMVTGTHGKTSTTSMLIVALQHCGYDPSFAVGGELNEAGTNAHHGSGDVFVAEADESDGSLLQYRPNLIIVTNIEADHLDHFGSVEAYSAVFDEFAETLGSEGVLVVCLDDPGAAALARRAHERGIRVRGYGSADQAEAGDVPVAGQLRDWQFKDTGATAQIQLAGESAPRTMRLSVPGRHMALNALAAVVTAAEIGAAVDDVLDGLAGFEGVRRRFELVGSVESVRVFDDYAHHPTEVRTVLQAVSGIVAQQGFGRSVVVFQPHLYSRTAAFATEFADALSIADLVFVLDVYGAREAPLPGVTGALIVEQIAGAPVHYLPDLSTVAQQVAAATAPGDLVITMGAGDVTLQGKEIVRALRARANDWPPPGNGR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that can perform ATP binding in cytoplasm to support cell wall organization, cell division, cell cycle.2. The protein should be optimized for activity in Cell wall biogenesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.4. The protein must exhibit the following characteristics: Cell wall formation.. The designed protein sequence is ",
    "response#1": "IVKSLGINTPDWETFISEGDVLNTEWNKFPAVVKPVREGSSVGLKIVESLEELKEYALDLLKKTERVMVEEFVEGRDMTVGILKGEALPVVEIIPKKGVYDYECKYTKGMSEYRILKDEKLSKKLQEISLKISKFLSLKDFARIDFRVTKEGKIFFLEVNTIPGMTELSLLPMAAKEKGMDFKKLISIIIK",
    "response#2": "IVKSLGINTPDWETFISEGDVLNTEWNKFPAVVKPVREGSSVGLKIVESLEELKEYALDLLKKTERVMVEEFVEGRDMTVGILKGEALPVVEIIPKKGVYDYECKYTKGMSEYRILKDEKLSKKLQEISLKISKFLSLKDFARIDFRVTKEGKIFFLEVNTIPGMTELSLLPMAAKEKGMDFKKLISIIIK",
    "response#3": "LMRDAGLPVVPFLTTTASSRIEYAAAVAALGTPDLFVKPANLGSSVGVSRARSEEEFAASCALAFRYDRKILVEQALNGAREIECSVLEESTGDIRASGLGEIVRASEHGFYSYQAKYIDADGAALHIPADLPLLQARRLQELAVEVFNVLCCEGMARVDFFVQGDEVFVNEANTLPGFTSSSMYPKLWEASGLSQTDLMDKLYG"
  },
  {
    "reference": "MNHFPLFADLRGRPVLLVGGGAVAARKAESLLKAGARVRVAAAELGAAMTAFHQAGCVEWSARTFDDALLDGVFLAVAATGDTALNRRVFAAAEARQKPVNTVDDASLCSFIFPAMIDRGDIQIAVSSGGKAPVLARLVREKLEALLPQGLGVMADLAGRWRSRVKQRLDSITQRRRFWEKLFADADFRRLCENSRTGEAETLLARSLDAGVTRRGSVALVGAGPGDAGLLTLKGLQKIQAADVVLYDALVSDGVLELIRRDAEKIFVGKRAGGKRVAQEETNALLVRLAQEGKRVVRLKGGDPFVFGRGGEELETLAAAGIAFEVVPGITAALGATAYAGIPLTHRDHAQSAMFVTGHVQPDGKALNWKTLAQGRQTLVVYMGAIRAAELAAALLEHGRSPDTPAAVISRGTQPQQRVSTGTLANLPALAAAAESPALIVIGETVALRRKLDWFGKTAERKPHRFRQEYFYAAPATVDDTCTAAAGL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1; Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme..2. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor, Proton donor to enable effective catalysis of NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin.3. The designed protein should be optimized for NAD binding, sirohydrochlorin ferrochelatase activity, precorrin-2 dehydrogenase activity activity in order to improve methylation, cobalamin biosynthetic process.4. The S-adenosyl-L-methionine, NAD(+) binding site should be stable and able to withstand changes in environmental conditions.5. A protein that can modulate the overall flux of metabolites through the sirohydrochlorin from precorrin-2: step 1/1, siroheme biosynthesis pathway, enhancing or suppressing its activity as needed.6. The designed protein must have at least one Tetrapyrrole methylase, Sirohaem synthase dimerisation, Siroheme synthase central domain. The designed protein sequence is ",
    "response#1": "MFARLEGRPVLLVGGGEVALRKARLLLAAGARLTLVSPVLASEFDELAGRFNHLPVRFEPAHLAGQLLVVAATDDNDVNALVYESATELGLFVNLVDDPARSSFIFPSIIDRSPIMVAVSSGGKAPVLVRLLRERLESLLPRHLGGLAELSGRVRHRAKELLSSISDRRRFWERAFASNALAGLIEKQDWQRAEAWLNAXLGXGAXGVRSPIQIAVSTGGASPVLARLLRARLESGAVALVGAGPGDPGLLTLRGLRVLNEADVILHDRLVSDEVLALARRDADRIELGKQVGGDHEATQARIHALLVEHARAGKRVVRLKGGDPFVFGRGGEELEVLHAHGIDFEVVPGITAAVACAAYAGVPLTHREHAQSVRFVTALGRDGTDPVDWPALARERQTLAFYMGVSGLTRLREQLIAHGRAPGTPFALVENGTRADQRVVTGTLDTLYPGXPAAAGSVALVGAGPGDPGLLTLRGLRVLNEADVILHDRLVSDEVLALARRDADRIELGKQVGGDHEATQARIHALLVEHARAGKRVVRLKGGDPFVFGRGGEELEVLHAHGIDFEVVPGITAAVACAAYAGVPLTHREHAQSVRFVTALGRDGTDPVDWPALARERQTLAFYMGVSGLTRLREQLIAHGRAPGTPFALVENGTRADQRVVTGTLDTLPEAAARHAVKAPALLILGEVAASARATXGXGXRXGXAXSX",
    "response#2": "MFARLEGRPVLLVGGGEVALRKARLLLAAGARLTLVSPVLASEFDELAGRFNHLPVRFEPAHLAGQLLVVAATDDNDVNALVYESATELGLFVNLVDDPARSSFIFPSIIDRSPIMVAVSSGGKAPVLVRLLRERLESLLPRHLGGLAELSGRVRHRAKELLSSISDRRRFWERAFASNALAGLIEKQDWQRAEAWLNALAAQPLMSPILVAISSAGKAPVLVRMLREKLEAAVGAGPGDPGLLTLRGLRVLNEADVILHDRLVSDEVLALARRDADRIELGKQVGGDHEATQARIHALLVEHARAGKRVVRLKGGDPFVFGRGGEELEVLHAHGIDFEVVPGITAAVACAAYAGVPLTHREHAQSVRFVTALGRDGTDPVDWPALARERQTLAFYMGVSGLTRLREQLIAHGRAPGTPFALVENGTRADQRVVTGTLDTLPEAAARHAVKAPALLILGEVGSVALVGAGPGDPGLLTLRGLRVLNEADVILHDRLVSDEVLALARRDADRIELGKQVGGDHEATQARIHALLVEHARAGKRVVRLKGGDPFVFGRGGEELEVLHAHGIDFEVVPGITAAVACAAYAGVPLTHREHAQSVRFVTALGRDGTDPVDWPALARERQTLAFYMGVSGLTRLREQLIAHGRAPGTPFALVENGTRADQRVVTGTLDTLPEAAARHAVKAPALLILGEVAAGQAGAAALARRLADFPLFIDLRDRRVLVVGGGAVARRKIDALLASGARVVVGAPTLEPGVAALVAEGRVEHIAGRFDPNWLNDAWLVVAATDNPIINRAVSTAAEARRVWANVVDDATLSSVQVPSRVERGPLQVAISSGGGAPMLARHLRERLETELDESLGDLAGLLDRERTRIRQRFPEIGARRRFFDALLAGPVPSHLRRGDTRAAQAAFDAA",
    "response#3": "MFARLEGRPVLLVGGGEVALRKARLLLAAGARLTLVSPVLASEFDELAGRFNHLPVRFEPAHLAGQLLVVAATDDNDVNALVYESATELGLFVNLVDDPARSSFIFPSIIDRSPIMVAVSSGGKAPVLVRLLRERLESLLPRHLGGLAELSGRVRHRAKELLSSISDRRRFWERAFASNALAGLIEKQDWQRAEAWLNQGLQSDALPGSVALVGAGPGDPGLLTLRGLRVLNEADVILHDRLVSDEVLALARRDADRIELGKQVGGDHEATQARIHALLVEHARAGKRVVRLKGGDPFVFGRGGEELEVLHAHGIDFEVVPGITAAVACAAYAGVPLTHREHAQSVRFVTALGRDGTDPVDWPALARERQTLAFYMGVSGLTRLREQLIAHGRAPGTPFALVENGTRADQRVVTGTLDTLPEAAARHAVKAPALLILGEVAAGSVALVGAGPGDPGLLTLRGLRVLNEADVILHDRLVSDEVLALARRDADRIELGKQVGGDHEATQARIHALLVEHARAGKRVVRLKGGDPFVFGRGGEELEVLHAHGIDFEVVPGITAAVACAAYAGVPLTHREHAQSVRFVTALGRDGTDPVDWPALARERQTLAFYMGVSGLTRLREQLIAHGRAPGTPFALVENGTRADQRVVTGTLDTLPEAAARHAVKAPALLILGEVAAQLGAQPLHFPAAGSVVLVGAGPGDPGLLTLKALRALNEADVILYDRLTSADVLALARRDADRVSVGKLPGENHDATQARIHALMVEHARAGRRVVRLKGGDAFIFGRGGEELEHLREHGVRYEVVPGVTAALACAAYAGIPLTHRDHAQSVRFVTAHCRQDEDTLDWDGLARGSQTLAFYMGVGQLEYLSERLITHGRAAETPFALVENGSRPEQRVVTGTLADLAARARAHGIHAPALLIVGEVA"
  },
  {
    "reference": "MKIFSAAQLHEADKITTQKHNITQLDLMERAGTQIFNWLHGRMQGAQVPVHIFCGIGNNGGDGLVVGRMLHEHGYNVKIYIANFTDKRSKCFLVNYDRVKDVTKNWPTLMTSEEDFPEISPEDIIIDALFGIGLNRPPEGWVKKLIQYINSTKSFKLSIDIPSGMYADKALDDPEAVVRANHTLAFQAPKLSFFLPETGKFVPYFEVLEIGLDPEYLHTTEPLAQIIAKPEAQQFYKQREKYAHKGTYGHTLVIAGSYGKIGAAVLSAKASYKTGSGLVTAFVPECGYTVLQTAVPEAMTLTDKEDDLITEIKLDFEPSAIAVGMGIGTKAETVRALEKLFTTTKSPMVIDADALNCISENKSLLEKLPELSILTPHPGELKRLVGDWKDDFEKLEKTKAFAKQHNVIIIVKGANTIVAIENRLYINTTGNPGMATGGSGDVLSGMIAGLLSQGYDPLIASVFAAYLHGSAGNQATHTLGFEALTASDIVEHIGAAFLELFAMKQEEDKNKNA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have a high affinity for (6S)-NADPHX, K(+).2. The designed protein must have at least one YjeF N-terminal domain.3. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..4. The protein must exhibit kinase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity. The designed protein sequence is ",
    "response#1": "MYAADQAAMAAGIPGIRLMASAGAAVASAVQGLVGEGARPRVLALCGPGNNGGDGFVAAGLLAAAGWPVRVALLGEREALTGDAAWAATQWPGTVEPATPALLADADIVIDALFGAGLRRPLEGAALGLVQALAASGLPVVAVDVPSGVNGDTGQVMGAAAPAQVTVTFFRRKPGHLLLPGRQLCGRVELADIGIPPGLLG",
    "response#2": "LFAASEGKSGAAILAAEAAMRSGVGLLTAHVPKYVLPILQAGVPEAMCRKDDDPYIVTHSGEVSMFNAIAFGPGVGTDPRTANALKMLIQNTINPLVIDADGLNLLAQNPTWLAFLPKGSILTPHPKEFERIAGKTQSGLHRLELAKDLARKFSIYIILKGAYTAVVCPDGRVFFNPTGNPGMATAGSGDVLTGIILAWLAKGYPPLQAALISVFLHGTAGDLAAARLGMEGMTARDIVFHR",
    "response#3": "LFAASEGKSGAAILAAEAAMRSGVGLLTAHVPKYVLPILQAGVPEAMCRKDDDPYIVTHSGEVSMFNAIAFGPGVGTDPRTANALKMLIQNTINPLVIDADGLNLLAQNPTWLAFLPKGSILTPHPKEFERIAGKTQSGLHRLELAKDLARKFSIYIILKGAYTAVVCPDGRVFFNPTGNPGMATAGSGDVLTGIILAWLAKGYPPLQAALISVFLHGTAGDLAAARLGMEGMTARDIVFHR"
  },
  {
    "reference": "MRIGYGYDVHGLVPGRKLVLGGVTIPYDRGLAGHSDADVLLHALADALLGAAALGDIGHYFPPGDPRYKDADSMLLLREVYRHVQTAGYRLANADATITAQEPRLAPYIDAMRERIAAALGVTKEQISVKATTTEGLGFTGRGEGISAQAVVLLLDREAGAGL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein have enhanced 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-   erythritol 2,4-cyclic diphosphate + CMP.2. The a divalent metal cation binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MRIGHGFDAHRLVEGRKLILGGVDIPYELGLAGHSDADVLVHALMDALLGALGAGDIGRHFPDTDEQYRGADSLTLLERVIALVKAKKMRLVNADITVVCQQPKLAPHLATMQANLREVCRAEEINIKATTTEEMGYTGRGEGITAHAVVLS",
    "response#2": "MRIGHGFDAHRLVEGRKLILGGVDIPYELGLAGHSDADVLVHALMDALLGALGAGDIGRHFPDTDEQYRGADSLTLLERVIALVKAKKMRLVNADITVVCQQPKLAPHLATMQANLREVCRAEEINIKATTTEEMGYTGRGEGITAHAVVLE",
    "response#3": "RVGNGFDVHRFGPGDHVVLCGVQIPHTRGLQGHSDADVGLHTITDAIYGALSEGDIGRHFPPSDPKWKGAASEIFLAHAADLSASRGFRISNIDCTLICEFPKIGPHAVAMQSEVARITRTDAARVSVKATTSEKLGFTGRGEGIACMATVTLL"
  },
  {
    "reference": "MKNEVVYKQLTEKILPFWNAMRDDENGGFYGYMSEDLHIDDHADKGCILNSRILWFYSTAYMYLQDEKLLDNAKHAFEFLKTYCFDPMCGGIFWSVRYNGKPADTTKHTYNQAFAIYALSAYYEATGSIEAIAIAEIIYEKIEDTMRDTKGYLEAFTRDFRPADNDKLSENGVMAERTMNTLLHIIEAYSALVHALRKKVADPAKGDVRDELFMNVVENKLAAALELMRDKFYNSDRHRLDVFFDKEYESLIDLTSYGHDIEASWLLEWAAGILDDEEITESLHPISSDLVEKVYKEAFDGHSIVNECEDGDVNTDRIWWVEAESVLGFLKAFEREGKEEYRKAAHEILAFILDKQVDKREGSEWFEMLKEDGTPCHKPMVREWKCPYHNGRMCLEILKSGIEIG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have cellobiose epimerase activity to facilitate carbohydrate metabolic process.2. For general function, the protein need meet that Can also epimerize lactose to epilactose.; Catalyzes the reversible epimerization of cellobiose to 4-O- beta-D-glucopyranosyl-D-mannose (Glc-Man)3. The desired protein should be able to perform D-cellobiose = beta-D-glucosyl-(1->4)-D-mannopyranose The designed protein sequence is ",
    "response#1": "YPHMWSWDAAFVAIGLAPLSVERAVVELDTLLSAQWRNGMIPHIVFANGVDGYFPGPARWATATLADNAPRNRLTSGITQPPVHAIAVQRILEHARTRGRSTRAVAEAFLDRRWGDLMRWHRWLAECRDRNERGRITLYHGWESGMDNSPRWDSAYANVVPGKLPEYQRADNVIITDPSQRPSDGEYDRYLWLLEEMKAVRYDDERLPSVMSFQVEDVFFSAIFSVACQVLAEIGEDYKRPHADVKDLYLWAERFRAGVVETTDQRTGAARDFDVLAEKWLVTETAAQFAPLLCGGLPHDRERALLKLLEGPRFCGHPDLKYGLIPSTSPVSRDFRPREYWRGPVWPVLTWLFSWCFARRGWAERARLLRQEGLRQASDGSFAEYYEPFTGEPLGSMQQSWGRIGIDDVAPVVSCGRYPAKAVVGEVVPVRATVWREGHDAVSATLVVRYLGTEFPRLASGPGTTPPAVPLGTVVQPGKRVKPQILQMSKGRTPDVFHGEFTPDAVGLWTFRVDGWGDPIATWRHAVEAKLEAGQSETELNNDLLVGARLLMRAAEGVPRKLRDPLLEAAQQLRTPGDPYQRAGGALSPEVADLLLQYPLREFVTRGEVHGVWVDPRIAIESVSPVVEEGAYPAKTESDRDLRLAARIFADGHEVLGAEVVWRRVGETAERRLPLLPEGNDFWSAQLRTPPCGRLYFRIEAWIDRFAGYRRELRAKHGARLPLDLELREGDELLQRCAERGGPEIAAACAPLAERLQACQSVEERVALWLAAQTGELLRLVGPREHLVRSREYPVEVE",
    "response#2": "VPYLHSLGVDWVYLSPVLTAEQGSDHGYDVTDPSAVDPERGGPEGLAAVSKAARAAGMGVLIDIVPNHVGVATPAQNPWWWSLLKEGRQSRYAEAFDVDWDLAGGRIRLPVLGSDDDLDQLEIRDGELRYYDHRFPLAEGTYAEGDAPRDVHARQHYELIGWRRADNELNYRRFFAVNTLAGVRVEIPAVFDEAHQEVVRWFREDLADGLRIDG",
    "response#3": "ELFPRSESGDPTRHGTFDDVIRRLPQIAAMGFDVLYFPPIHPIGRTHRKGRNNSLRAEAGDPGSPYAIGSEEGGHEAIHPELGDREDFRRLLVAVREHGMELALDFAIQCSPDHPWLREHPGWFAWRPDGSLRYAENPPKKYEDIVNVDFYAEQALPSLWEALRDVVLGWVEQGVTLFRVDNPHTKPLPFWEWLIAEVRGRHPQVIFLSEAFTRPAMMARLGKVGFSQSYTYFTWRNDKQELAEYFAELNQPPWRDCYRPNFFVNTPDINPWFLQRSGRPGFLIRAALATMGSGLWGMYSGFELCEAAALPGKEEYLDSEKYQLRPRDYQAPGNIVAEIARLNRIRRENPALQTHLGFQAYNAWNDRILYFGKRTADLANFVLVAVCLDPHEAQEAHFELPLWEFGLPDDASLQGEDLMNGHRWVWHGKVQWMRIEPWR"
  },
  {
    "reference": "MKIFLPVLVMLILCSMCLLTEGQVSTNKKCSNTSQCYKTCEKVVGVAAGKCMNGKCICYP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: May also inhibit Kv4/KCND when coexpressed with DPP6 or DPP10; It shares the same target in rat brain than BmTX3 (AC Q8I0L5) and AmmTX3 (AC P60208).2. The protein must be able to potassium channel regulator activity. The designed protein sequence is ",
    "response#1": "VVSYLCAMVIFTLTAYYQPLEGTPPYPYRTTVDYLRLAGEVITLFTGVLFFFTSIKDLFTKKCPGVNSLFVDGSFQLLYFIYSVLVVVSAALYLAGIEAYLAVMVFALVLGWMNALYFTRGLKLTGTYSIMIQKILFKDLFRFLLVYLLFMIGYASALVTLLNPCTNMKVCDEDQSNCTVPTYPACRDSETFSAFLLDLFKLTIGMGDLEMLSSAKYPVVFILLLVTYIILTFVLLLNMLIALMGETVGQVSKEL",
    "response#2": "SPDLKYVLLAYDVKQIFHYSFTASYLIYNIHTGEVWELNPPEVEDSVLQYAAWGVQGQQLIYIFENNIYYQPDIKSSSLRLTSSGKEGIVFNGIADWLYEEELLHSHIAHWWSPDGERLAFLMINDSLVPNMVIPRFTGALYPKAKQYPYPKAGQANPSVKLYVVNLYGPTHTLELMPPDIFKSREYYITMVKWVSNTRTVVRWLNRPQNISILTVCESTTGACSRKYEMTSDTWISKQNEEPVFSRDGSKFFMTVPVKQGGRGEFHHIAMFLVQSKSEQITVRHLTSGNWEVIRILAYDETTQKIYFLSTEFSPRGRQLYSASTEGLLSRDCISCNFRKEDCTYFDASFSPMNQHFLLFCEGPKVPIV",
    "response#3": "VVSYLCAMVIFTLTAYYQPLEGTPPYPYRTTVDYLRLAGEVITLFTGVLFFFTSIKDLFTKKCPGVNSLFVDGSFQLLYFIYSVLVVVSAALYLAGIEAYLAVMVFALVLGWMNALYFTRGLKLTGTYSIMIQKILFKDLFRFLLVYLLFMIGYASALVTLLNPCTNMKVCDEDQSNCTVPTYPACRDSETFSAFLLDLFKLTIGMGDLEMLSSAKYPVVFILLLVTYIILTFVLLLNMLIALMGETVGQVSKEL"
  },
  {
    "reference": "MTAADRADAATDVGDPGRSAAGGRRLTVVVGVSGGIAAYKAVQVVRGLVVLGHDVHVVPTEAALRFVGLPTFEAISRNPVHPSVWDDVAQVRHVALGRAADVIVVVPATAHTLAKIAHGLSDDLLGTTILSSAAPLVVAPAMHSEMWAAAATVANMDLLRGRGVVVVGPDDGPLTGGDSGPGRLADPDEILAATLAAVVPAAREEARVRDLAGRVVVVTAGGTREPLDPVRFLGNRSSGRQGVALARAAARRGATVTLVAAHLDDDVRRDAVGDDGVSLVEVGSALELREAVVAAAAGADVVVMAAAVADYRPVEVSEAKIKKDDTGDELVLRLVRTPDVLAELSATRRPGQVVVGFASETVTDRDELLELGRRKLARKGCDLLVVNPVGWATGFGSPDTVVLVLGADGAVVGEVAGTKQQAAEVVLDAALAVRAAGALAR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The desired protein should be able to perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO22. The CTP binding site should be located in a region of the protein that is accessible to the ligand.3. A protein with phosphopantothenate--cysteine ligase activity, FMN binding, phosphopantothenoylcysteine decarboxylase activity, metal ion binding is needed to enhance pantothenate catabolic process, coenzyme A biosynthetic process in a specific context.4. The protein should be optimized for activity in coenzyme A biosynthesis, Cofactor biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.6. Mg(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "MNIVLGVGGGIAAYKSALLLRLFTEAGHKVTVIPTEASTKFVGVATWEALSGRPATNDVFDAVDQVNHVRIGHEADLIVVAPATADLLAKAAGGQANDLLTTTLLMARGPVLFAPAMHTEMWRHAATQANVATLRSRGIHVLDPAVGRLTGPDTGPGRLPEPEAIFAAAAVARLLLDDADSLPYGRHVVITAGGTREPLDPVRFLGNKSSGKQGVALAESALAAGATVTFIHAHMDVAPPAGATLQAVETALELRSATLAAAGSADVVIMAAAVADFRPSNVSTGKIKKRDNVADPVINLVRNPDILVEVVAHRAAQNQRQLIVGFAAETGDAESDALGYAREKLRRKGCDLLVVNEVGPGESGAEKVFGQDANRVHILDATGVEDWPDLSKDEVADRLLTRVAAALG",
    "response#2": "MNIVLGVGGGIAAYKSALLLRLFTEAGHKVTVIPTEASTKFVGVATWEALSGRPATNDVFDAVDQVNHVRIGHEADLIVVAPATADLLAKAAGGQANDLLTTTLLMARGPVLFAPAMHTEMWRHAATQANVATLRSRGIHVLDPAVGRLTGPDTGPGRLPEPEAIFAAAAAAEALTDPAALPRLAGRHVVVSAGGTREPLDPVRFLGNRSSGKQGYALARTAAARGARVTLLSANAALPDPAGVDVVHVTTARELRDAVRQAAGTADAVVMAAAVADFRPAAYATGKIKKQEGKEPEPVALVRNPDILAELAADRARPGQLIVGFAAETDDILANGRAKLRRKGCDLLVVNEVGENKTFGAEESEAVVLGADGSEHVLPAASKLEMAHRVWDLVAPRLAAR",
    "response#3": "MNIVLGVGGGIAAYKSALLLRLFTEAGHKVTVIPTEASTKFVGVATWEALSGRPATNDVFDAVDQVNHVRIGHEADLIVVAPATADLLAKAAGGQANDLLTTTLLMARGPVLFAPAMHTEMWRHAATQANVATLRSRGIHVLDPAVGRLTGPDTGPGRLPEPEAIFAAAAALTVLAGPAAALAGRHVVVSAGGTREPLDPVRFLGNRSSGKQGYALARTAAARGARVTLLSANAALPDPAGVDVVHVTTARELRDAVRQAAGTADAVVMAAAVADFRPAAYATGKIKKQEGKEPEPVALVRNPDILAELAADRARPGQLIVGFAAETDDILANGRAKLRRKGCDLLVVNEVGENKTFGAEESEAVVLGADGTVTDVPHGSKAALAATVWDTVAARLT"
  },
  {
    "reference": "MKSNRIFISWLRWPLFIRIGVIILCLILLFGQIIYILEPKQFTSVFEGIWWAVVTVSTVGYGDYVPHTPLGQAAGILLILSGASFVTAYFATLSAAAFSRQHRYIEGKVAYKGRDHIILIGWNEKTNRLLKDLQLAAPSKTVVLIDESLTEGPLIENVHFIRGHAADDGTLKRANITEAESVMITADQYKSETDADMLSVLTLLSVKGLNPLAYCIVEILTDRFVTNAERAGANQIIGTSEFISRAMLQHYQVKLRPSKQQNGIKLTLDQHVELLAVPDELKGAAYKTCVLYFLDHNTTIIGIQKKEGPMLSPPLTYKVLETDQFLAI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein have RCK N-terminal domains with specific sequence motifs that are crucial for its activity.2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.3. The designed protein should be expressed specifically in the plasma membrane The designed protein sequence is ",
    "response#1": "MPGTIGGLALRVGQFLFAAAAIVIMVTGDEFTNYTAFCYLVAAMSLQFLWSFMLAILDTYALLIKRGLRNSVLLSLFVVGDWVTATLSLAAACSTAGVTVLFDNDLNYCGQMHCHRYQLSAAMAFLSWLL",
    "response#2": "MPGTIGGLALRVGQFLFAAAAIVIMVTGDEFTNYTAFCYLVAAMSLQFLWSFMLAILDTYALLIKRGLRNSVLLSLFVVGDWVTATLSLAAACSTAGVTVLFDNDLNYCGQMHCHRYQLSAAMAFLSWLI",
    "response#3": "MPGTIGGLALRVGQFLFAAAAIVIMVTGDEFTNYTAFCYLVAAMSLQFLWSFMLAILDTYALLIKRGLRNSVLLSLFVVGDWVTATLSLAAACSTAGVTVLFDNDLNYCGQMHCHRYQLSAAMAFLSWLI"
  },
  {
    "reference": "MFSNWNIFLNYKNFTINQEIKNKLNLYYQILIEENQKYNLTRITELNEVFEKHFLDSLLFVEQFQIIDQKIADIGTGAGFPGIVLKIFFPNIKLTLIESNNKKVNFLKYLVQKLNLNDVEILNKRAEELNEYKEQFDIVISRAVAYLNIILELGVQLVKVNGMFILLKGPKAYQEIKDLKNKDQKMNLKLINIQELEDTGFGTRINLFYKKINHTNNLYPRKYQQILKESK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Specifically methylates the N7 position of a guanine in 16S rRNA..2. The protein should be able to bind S-adenosyl-L-methionine ligand in a variety of conditions3. The protein should have rRNA (guanine-N7-)-methyltransferase activity and be active in cytoplasm. The designed protein sequence is ",
    "response#1": "LGYKISLWTRLATRIMLKLGEGEAKDKDELFKAASSINWPDIFKSTTTFAVDFVGYSEEIRNSQFGGLTIKDAIVDQFRDQGFERPNVDKKAPQISFQARLLRDNVTIFLDK",
    "response#2": "LGYKISLWTRLATRIMLKLGEGEAKDKDELFKAASSINWPDIFKSTTTFAVDFVGYSEEIRNSQFGGLTIKDAIVDQFRDQGFERPNVDKKAPQISFQARLLRDNVTIFLDK",
    "response#3": "YAAPKTIEEDKAKKRLQEVIYWAPKVLNIPTDKVILKTRAKQRGANQYERLEKTKQSLTINEHGALFKINLWDYLDTGLFLDHRKTRQIVAKKSQGKSLLNLFAYTGSVSVQAAVQGAASITTVDMSNTYLNWAEDNFALNKLNGHKYQFIQADCLDWLKKNVNKFDVIFIDPPTFSNSKRMEDSFDVQRDHVDLITDALKSLNRGGEIFFTNNKRNFKIDC"
  },
  {
    "reference": "MDHQVSLPQSTTTGLSFKVHRQQRELVTPAKPTPRELKPLSDIDDQQGLRFQIPVIFFYRPNLSSDLDPVQVIKKALADALVYYYPFAGRLRELSNRKLAVDCTGEGVLFIEAEADVALAELEEADALLPPFPFLEELLFDVEGSSDVLNTPLLLVQVTRLKCCGFIFALRFNHTMTDGAGLSLFLKSLCELACGLHAPSVPPVWNRHLLTVSASEARVTHTHREYDDQVGIDVVATGHPLVSRSFFFRAEEISAIRKLLPPDLHNTSFEALSSFLWRCRTIALNPDPNTEMRLTCIINSRSKLRNPPLEPGYYGNVFVIPAAIATARDLIEKPLEFALRLIQETKSSVTEDYVRSVTALMATRGRPMFVASGNYIISDLRHFDLGKIDFGPWGKPVYGGTAKAGIALFPGVSFYVPFKNKKGETGTVVAISLPVRAMETFVAELNGVLNVSKG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of (3Z)-hex-3-en-1-ol + acetyl-CoA = (3Z)-hex-3-en-1-yl acetate +   CoA.2. A protein with acetyl CoA:(Z)-3-hexen-1-ol acetyltransferase activity, (Z)-3-hexen-1-ol acetyltransferase activity is needed to enhance green leaf volatile biosynthetic process in a specific context.3. The protein must exhibit the following characteristics: Uses acetyl-CoA as substrate, but not malonyl-CoA or benzoyl-CoA; Acyltransferase involved in the production of green leaf volatiles (GLVs). The designed protein sequence is ",
    "response#1": "PDLTFIRHRQVEFYTLVSGIDMEPKRSTFRINFVKICHFVTILDDMYDTFGTIDELRLFTAAVKRWDKSATECLPEYMKGVYIDLYETVNELAREAYKSQGRDTLNYARQALEDYLGSYLKEAEWISTGYIPTFEEYLVNGKVSSAHRIATLQPILMLDVPFPPHVLQEIDFPSKFNDLAGSILRLRGDTRCYQNDRARGEEASCISCYMKDNPGSTEEDALNHINGMIEKQII",
    "response#2": "EKLLELAKVEFNIFNSLQQNELKCVSRWWKESGSPDLTFIRHRQVEFYTLVSGIDMEPKRSTFRINFVKICHFVTILDDMYDTFGTIDELRLFTAAVKRWDKSATECLPEYMKGVYIDLYETVNELAREAYKSQGRDTLNYARQALEDYLGSYLKEAEWISTGYIPTFEEYLVNGKVSSAHRIATLQPILMLDVPFPPHVLQEIDFPSKFNDLAGSILRLRGDTRCYQNDRARGEEASCISCYMKDNPGSTEEDALNHINGMIEKQIKELNWELLKPDKNVPISSKKHAFNISRGLHHFYKYRDGYTVANSETRNLVIKTVLEPG",
    "response#3": "EKLLELAKVEFNIFNSLQQNELKCVSRWWKESGSPDLTFIRHRQVEFYTLVSGIDMEPKRSTFRINFVKICHFVTILDDMYDTFGTIDELRLFTAAVKRWDKSATECLPEYMKGVYIDLYETVNELAREAYKSQGRDTLNYARQALEDYLGSYLKEAEWISTGYIPTFEEYLVNGKVSSAHRIATLQPILMLDVPFPPHVLQEIDFPSKFNDLAGSILRLRGDTRCYQNDRARGEEASCISCYMKDNPGSTEEDALNHINGMIEKQIKELNWELLKPDKNVPISSKKHAFNISRGLHHFYKYRDGYTVANSETRNLVIKTVLEPV"
  },
  {
    "reference": "MVKATSVKDVDQHEIVQHIAKFLKKSGKVKVPEWSDVTKMGISKELAPLNSDWYYVRTASIARRLYVRSPTGVDALRLVYGGSKRRGVVPNHFAKASGSVIRKALQTLEAIKWVQKHPDGNGRVLTKQGRKDLDRIASQMRQNDRFTA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must have high specificity towards structural constituent of ribosome.2. For general function, the protein need meet that Elimination of the ALEP-1 gene from all somatic cells in its fully activate state may represent an alternative way to gene regulation. The designed protein sequence is ",
    "response#1": "GKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQKNG",
    "response#2": "KTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWH",
    "response#3": "SVKKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRL"
  },
  {
    "reference": "MTELFIDGAWVAGSGPVFASRNPGTDAVAWQGESASAADVDRAVASARRAFAGWSALDFEARCEIVKRFAALLTERKEAIATAIGRETGKPLWEARTEVASMAAKVGISIQAYQERTGEKRQDMADGVAVLRHRPHGVVAVFGPYNFPGHLPNGHIVPALIAGNTVVFKPSELAPGVARATVEVWQEAGLPAGVLNLVQGEKDTGIALANHRQIDGLFFTGSSDTGTLLHKQFGGRPEIVLALEMGGNNPLVIGEVEDIDAAVHHTIQSAFLSAGQRCTCARRIFVPQGAFGDRFLARFADVTSKITADVFDADPQPFMGAVISARAAAKLVDAQARLVEQGAKPIVAMAQRDPRLGFVNAAIVDVTGVANLPDEEHFGPLAQVVRYATFDEAIERANDTAFGLSAGLLADDAKVWEHFRRTIRAGIVNWNRPTNGASSAAPFGGTGRSGNHRPSAYYAADYCAYPMASVESTQLTLPASLSPGLHF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The NAD(+) binding site should be located in a region of the protein that is accessible to the ligand.2. The designed protein should be optimized for succinylglutamate-semialdehyde dehydrogenase activity activity in order to improve arginine catabolic process to succinate.3. For general function, the protein need meet that Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate. The designed protein sequence is ",
    "response#1": "MVNGAGLAMATMDIIKLNGAFPANFLDVGGGATTEKVTAAFKIILKDPAVEGILVNIFGGIMRCDTIAEGIVVAAKEVELDVPLVVRLEGTNVEKGKDILANSGLPIVPADDLGDAARKY",
    "response#2": "WSQGQGTEFSSVNPANNNVIFQANSATAEQVDAAVSAAREAFYAWADKTFAERLEIVKAFAAQLKENSEELAITIAQETGKPLWETRTEAGAMVGKIAISEKAFLERTGDVENAMPLGRAMIRHKPHGVVAVFGPYNFPGHLPNGHIVPALLAGNTVIFKPSELTPKVAELTLKLWEKAGLPAGVINLVQGEVATGKALAAHKGIDGLFFTGSSRTGHILHEQFAGQPGKILALEMGGNNPLIITDVEDTKAVVHDIIQSAFISSGQRCTCARKLFLPTGSKGDVILERLITATKAIKVGNYDDADQPFMGSMISSAAAAGMVKAQNELVELGAQVLVELEHTVNTGFVTPGIIECTNISDFPDEEHFGPLLKVFRFDDFDQAIDKANDTSFGLSAGLLSDSAADYEHFLRRIRAGIVNWNRPITGASSAAPFGGIGASGNHRASAYYAADYCAYPVA",
    "response#3": "MVNGAGLAMATMDIIKLNGAFPANFLDVGGGATTEKVTAAFKIILKDPAVEGILVNIFGGIMRCDTIAEGIVVAAKEVELDVPLVVRLEGTNVEKGKDILANSGLPIVPADDLGDAARKY"
  },
  {
    "reference": "MTETVNSARATARFVRVSPMKARRVIDLVRGKSVEDALAILKYAPQAASEDVAKVVASAAANAENNFGLDPRTLVISEAYADEGPTMRRFRPRAQGRAFHIRKRSSHITVVVESQKGSAQ",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have rRNA binding within large ribosomal subunit to regulate translation. The designed protein sequence is ",
    "response#1": "MGRMHAPGKGLSSSALPYRRSVPTWLKLSSEDVKEQIYKLAKKGLRPSQIGVILRDSHL",
    "response#2": "MIVNLSQLERVFEAGATVDENTLRAAKLVQGPCDAVKLLGNGTLTKNLNVVVDFVSASAREKVTQAGGTVL",
    "response#3": "EIVNLETLNKFEDGAEVTPALLVESGIIKDEKDGIKVLGNGTLNKQLTVKASKFSASAKEAIESKGGKAE"
  },
  {
    "reference": "MPQGDHIELHQKRFGRRLDHYEKVRKKTARAAHKRSAIAQKVTGLKAKLYNKKRYSEKAEMKKTIAMHQERTNKKASDDKVKEGAVPAYLLDREGVSRAKVLSNMVKQKRKEKAGKWDVPLPKVRAISEDEMFKVVKSGKRQKKAWKRFVTKVTFVGEGFTRKPPKYERFIRPTGLRFKKAHVTHPELKSTFYLDILSVKKNPQSPTYTQLGVITKGTILEVNVSDLGLVTQTGKVVWGKMAQVTNNPENDGCINAVLLV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should include a Nuclear localization signal motif to ensure proper function.2. The protein must exhibit the following characteristics: Involved in the biogenesis of the 60S ribosomal subunit.3. The protein should be optimized for stability and expression in preribosome, large subunit precursor, nucleolus where it will participate in maturation of 5.8S rRNA, maturation of LSU-rRNA. The designed protein sequence is ",
    "response#1": "YQGDQEHIIHNDKVRSQRRMRAFERNINQFKWSEALDNAFVPGMAKELTLTVLQELRKRGKVRVALYGRDESTLEPLLNWCLKGIEDVRSASIVADWVAVVLELYGNTLESSPVLQELMIDLKTKVRHEIHKSKEAQRIEGMLQLN",
    "response#2": "YQGDQEHIIHNDKVRSQRRMRAFERNINQFKWSEALDNAFVPGMAKELTLTVLQELRKRGKVRVALYGRDESTLEPLLNWCLKGIEDVRSASIVADWVAVVLELYGNTLESSPVLQELMIDLKTKVRHEIHKSKEAQRIEGMLQLA",
    "response#3": "GKELKLATSQICSKLMERIILAADDQQLKAIFQGINGFFVNLSYNKYASHVVETLLVRSAALIEKELLTPVFEAELDGDEQVYASMENMFLFMLNELKPHMKMMINHQYASHVFRLLILVLSAKKLPKSTQSNSALRSKKSKIARKMVDLKDNADFDRTYRTPDSFKLELKGILVMLYKQFTNGIEPGTKHSEVNIACVTKFREYCVDKVASPVIQLIIQVEGIFDRDRSFWNLVFSNTEQNDPKEESFMEYLLSDSVGSHFLQAVIGFARTKQVERLYKLYMQDRIVKLAKRDTTGAFVIMSLLQNLGSKEVKSILDDLVPELSILLNSNLDFGSEIIEASIRQGDYKKTEIVEQLGKKYYPADSESKNILESCLQLASSTLGNTKDDWPTADERRRALFLEKLINFDDYFLEIAVESLLNLPEERIMQMCYHGVFSHVVEHVLQAKRVETVKRKLLLNILYKDAVNLACNAYGSHIMDKLWEFTAKLTLYKERIANLLLEEADKVKNSIYGL"
  },
  {
    "reference": "MHLLPEQATRHAVSIPELLVSRDERQARQHAWLTRHLTPLVSFTVVAPGPIKDSELTRRIFNHGVTALLAVAKKAGWSVREQAALASASGPEGFLAIEAPARDLKLATIELEHQHPLGRLWDIDVLTPEGDILSRRHFSLPARRCLLCEQSAAECARGKTHALSDLLIRMEALLHDADSSHIN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Transfers 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A on a serine residue to the apo-acyl carrier protein (gamma chain) of the citrate lyase to yield holo-acyl carrier protein.2. A protein with holo-citrate lyase synthase activity functions is required to enable prosthetic group biosynthetic process.3. The protein should have a low Km value for 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA + apo-   [citrate lyase ACP] = diphosphate + holo-[citrate lyase ACP]. The designed protein sequence is ",
    "response#1": "MXDVFISYAREDKPRVEQLVAALRAEGYTVWADRDISGGAEWQAAIDQAIRGCSAFVALWSAPSVDSAWVRKEAGYAQQAGKLIPVRMDNVEPPMGFRRVQAADLRGWQGDPHHPGLLALAAAVRAAIGGAPAAPQAPALPPAPKRPPWWLGAAALAAVAVAALYWLLRPAPAPVTQATPAATPAAPAAKPASAPVLVEERFAPGAKATGVPEGWTVTAEGGALDVKAGAARPAWLALASGTSRDWTIEADVMPLACEGDAGIVFGVQGAGDYYRFLVGADGRYALGRVEGDKLTELAPLAWTRQTHAGQANRLRVVAAGDEIRCFANGAFLGSATDASYRSGRAGVMAFGSGAQVRFRNLRVRAL",
    "response#2": "MNNEKLVIASNNQGKIKEISALFQPLGIRVIPQSDFGLPEPEETDDTFLANARIKATHACKLTGLPALADDSGICAHALDGAPGVHSARYAGENATDQDNNDKLLHALKGENNRHAHYYCILVYVRHANDPTPVIAEGQWSGEILTQPVGEGGFGYDPLFWVPKHQCSAAELTAEQKNRLSHRGKALAKLVEQLKL",
    "response#3": "MSIRLAFPSDTSAAQPSASVPAAPA"
  },
  {
    "reference": "MAKKHRYTTVQRKQERQKEEEYIKELEQRIQDYDVKNNKAVFFKDLPISKSTLKGLNEASFIKMTDIQRDSIVTSLQGHDVFGTAKTGSGKTLAFLVPVLEKLYRERWTEFDGLGALIISPTRELAMQIYEVLVKIGSHTQFSAGLVIGGKDVNFELERIAKINILIGTPGRILQHMDQAVGLNTSNLQMLVLDEADRCLDMGFQKTLDAIVGNLPPDRQTLLFSATQSQSISDLARLSLTDYKKIGTIDSSEDGPATPKTLQQSYIIADLADKLDVLYSFIKSHLKTKMIVFFSSSKQVHFVYETFRKMQPGISLLHLHGRQKQRARTETLDKFFRAQQVCLFATDVVARGIDFPAVDWVIQVDCPEDVDTYIHRVGRAARYGKKGRSLIILTPQEEAFLTRMAAKKIEPGKLTIKQSKKKSIKPQLQSLLFKDPELKYLGQKAFISYVKSIYIQKDKEVFKFDELPTEEFANSLGLPGAPRIKIKGMKAIEQAKKLKNTSRSLLSLSKANDDGEINDKKDKQVRTKYDKMFERKNQTILSEHYLNITKSQAQEDEDEDFITVKRKDHELKEEDLPQLTVPTSRRAQKKALSKKASLSTKGNATKMVFDDEGQAHPVYELEGEEEFHKKGDAEEQKKEFLSKEAEIMADRDVSDKIIQKEKKQEKKRKRLEAMRREMEAAYADEYSDEDEEGGNVAYLGTGNLSDDMEEYSSDEESRKTKKSKTVDYRFDKKNKTISEDTDIMEIQEPETIEDLESLTARLIEG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Incorporating a DEAD box motif is critical for the successful design of the protein.2. For general function, the protein need meet that ATP-dependent RNA helicase required for ribosome biogenesis; Involved in the release of U14 snoRNA in pre-ribosomal complexes; Required for pre-rRNA cleavage at site A2 .3. The designed protein must be optimized for U3 snoRNA binding, box C/D RNA binding, identical protein binding. The designed protein sequence is ",
    "response#1": "GLRQLLHPTARTATDVHLRPPFLIFTQTIPRAVALHSELLYDIPPEAGGSSRIAVLHSELSDSQRSEIMKGFRKGEIWILVTTDLLARGVDFRGINGVVNYDIPNSAAVYVHRVGRTGRAGREGGVAVTYYTKEDIPYVKSIANIIDVSEKLRGAGGEKSVQKWLLDSL",
    "response#2": "KPGETVEPDLLVVAPTGSGKTLSFLIPVINKIVRHHHEQPDERGIFAVVVAPTKELASQIVNEGRKLVQGTGVKITLMKKGMQVVERDGDGDSDEKDVLDEDSADSGSDSEDDEQTTDKKTKGKAPVTKSDILVTTPLLLVNALSANRTKPLAALPLVRNIVLDEADVLLDPLFREQTLDIWRSCTHPELRASLWSATMGSNVEDLAKSTIQERKDS",
    "response#3": "AIQQRAIIPIIRGRDVIAQAQSGTGKTATFSISMLQSIDTNLRETQALVLSPTRELAVQIQTVVLALGDYMNVSCHACIGGTSVGEDIRKLEAGQQVVSGTPGRVFDMIRRRNLRTKDIKMLILDESDELLNKGFKDQIYDIYRYLPPATQVVVVSATLPHDVIRVSAAFRSQALADREIFQMAQLAFVSWTRSYIEHQASSIFRIADLDWLDLAKGYGLLELPKMPSR"
  },
  {
    "reference": "MSTLFIGDIHLSVEYPEITKNFFRFLQTEATQAESLYILGDLFDKWIGDDDPHPFYREVAAELFALKKKGIPCYFIHGNRDFLIGQAFADESGMILLPECKVIQLYDYNICILHGDTLCTKDTEYQIFRKIAHHRFIQRLFLCLPLSFRFYIAKKLRLQSGEQNKKKGEEIMDVDPQSVLDLMACHDVDWIIHGHTHRPAIHNIEMIVTPLLSSKKIARRAVLSAWHNEGSMIKVTFDAIELIFFPFRVSDFEKI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The ideal protein would be one that plays a critical role in lipid A biosynthetic process through manganese ion binding within extrinsic component of plasma membrane.2. The desired protein should be able to perform H2O + UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-   glucosamine = 2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-   glucosaminyl 1-phosphate + 2 H(+) + UMP3. A protein should be able to manipulate the activity of enzymes in the lipid IV(A) biosynthesis, lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 4/6 pathway, either by activating or inhibiting their function.4. The designed protein should have a binding site that is specific to the target Mn(2+) ligand. The designed protein sequence is ",
    "response#1": "MRTLFIGDLHLSADRLDITQAFNRFLDTELDDADALYILGDLFEVWVGDDLAAPFALELARRLKQISQRLPIYFIHGNRDFMLGKQFADAAGMQILPEVTCLDLYGVNTVILHGDSMCTLDKAYQRFRKLRSFAFARWLYSCLPKRKRQAIADKIRSNSQSSNQQKSYVIMDVEPSAVDALFAQTHCKQMIHGHTHRPVVHTLSLGNGEARLVLGDWRDNGQGAVRILGEDGALLHEIPVAP",
    "response#2": "MTTLFISDLHLTPSRTDITECFVQFMRNEAVNAEALYVLGDLFEFWIGDEDCTPFAERIRNEFKALTTSGVPVYFIQGNRDFLLGQRFCRETGITLLDDVCTIDLYGEKVVILHGDTLCIDDLKYQEFRKTVHQPWLQWIFKRIPWFIKKRIVAKVQSGVRDDKQHKSLEIMDVNQQEVAQVMSQFCVKLMIHGHTHRPAIHQLLVGKKKCNMTTLFISDLHLTPSRTDITECFVQFMRNEAVNAEALYVLGDLFEFWIGDEDCTPFAERIRNEFKALTTSGVPVYFIQGNRDFLLGQRFCRETGITLLDDVCTIDLYGEKVVILHGDTLCIDDLKYQEFRKTVHQPWLQWIFKRIPWFIKKRIVAKVQSGVRDDKQHKSLEIMDVNQQEVAQVMSQFCVKLMIHGHTHRPSIHQLEVDGVTQRRIVLGDWYEQGSCLTIDGERMEILSFK",
    "response#3": "MRTLFIGDLHLSADRLDITQAFNRFLDTELDDADALYILGDLFEVWVGDDLAAPFALELARRLKQISQRLPIYFIHGNRDFMLGKQFADAAGMQILPEVTCLDLYGVNTVILHGDSMCTLDKAYQRFRKLRSFAFARWLYSCLPKRKRQAIADKIRSNSQSSNQQKSYVIMDVEPSAVDALFAQTHCKQMIHGHTHRPAIHALASGKAAILLISDLHLEEERPDITRAFLELLGGRARAAEALYILGDFFEVWIGDDAMTPYQLSICQALRELSDSGTQIFLMHGNRDFMLGKAFCKAAGATLLKDPSVVDFYGEPVLLMHGDSLCTRDEAYMRMRRYLRNPLSLWILRHLPLGTRRKLARKLRNESRAQTRMKANDIVDVTPDQVPQVMQEHGVRTLVHGHTHRPAIHALQDGVAAMTTLLISDLHLHTGQPDITRGFLDYLERDARHADTLYLLGDIFEAWIGDDYLGDLERQVIEALRRLSDAGTQLYFMHGNRDFLVGEDFAAAAGATLLDDPCLATLGRQTVLLMHGDSLCTGDEEYMKFRAMARDPEWQAQILALPIEQRLELAKSLRMQSSDANTQKADAIMDVTPAEVEAIMREHGVSTLIHGHTHRPAIHELTDGTRVLNPGEAGDPRPFVLGNGEMSLIDLTTMRTRIHRI"
  },
  {
    "reference": "MFHTIEEAIHDLKEGRPVIVVDDENRENEGDLVALSEKATPDVINFMITHAKGLVCTSIKPDLAKKLKLPLMSNDSSDPFETAFTVSIDHRETATGISTEDRSKTIRALTHPDTVAADFKRPGHVFPLIAKGGGVLERPGHTEASADLAALCGAFPSGFICEIIKDDGTMARVPDLQEMAEIFDLKLITIADLITYRKQYEMHVTREVETTLPTAFGTFRVYGYTNDLDGKEHIALVKGKINPKEPVLTRVHSECLTGDVFASYRCDCGPQLNEALKQIDEAGSGVLIYMRQEGRGIGLLNKLRAYHLQDDGMDTVEANEQLGFAPDMRDYAISAQILTDLGVTQVKLLTNNPQKLDELQSYGIQVEKRIPLETDVRQENEHYIYTKVHKMGHLLQSQQN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..2. The protein must have high specificity towards magnesium ion binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity.3. The protein design should consider the endogenous proteins and enzymes present in the 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway to avoid interference with native metabolic processes.4. The designed protein must have a Proton acceptor; for GTP cyclohydrolase activity that is structurally stable and resistant to denaturation.5. The protein should be able to bind Mg(2+), GTP, Zn(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "REAEANLPTKFGTFRIIAYRDLLSNIVHVALVKGKIKGKKDILVRVHSECLTGDVFGSLRCDCGEQLHSALRMIEREGEGVVLYMKQEGRGIGLLNKIKAYRLQEKGFDTVEANEKLGFPPDLRDYGIGAQILVDLGLSSIRLITNNPKKVVGLKGYGLYITERVSAEGPGLYQGERQAEADLPSDFGSFRAIGYRNELDGSEHVAIVKGHPAQAQGPVLVRVHSECLTGDAFGSLRCDCRPQLEAALRMVEAAGEGVVVYLRQEGRGIGLINKLKAYSLQDGGLDTVEANERLGFAADLRNYGVGAQILSDLGVQRLRLITNNPRKIAGLGGYGLRVEDRVG",
    "response#2": "REAEIRMPTDFGEFRMIGYTDRLTGQEHVALIKGGISDGRTVPVRIHSECMTGDVFGSRRCDCGPQLHAALRQMEEEGEGVLLYMRQEGRGIGLINKLKAYRLQEEGLDTVEANERLGFPDDMRDYAIAAQMLKDIGVGDIRLMTNNPRKLDGLTAYGIRITERLSGLAREAEANLPTKFGTFRIIAYRDLLSNIVHVALVKGKIKGKKDILVRVHSECLTGDVFGSLRCDCGEQLHSALRMIEREGEGVVLYMKQEGRGIGLLNKIKAYRLQEKGFDTVEANEKLGFPPDLRDYGIGAQILVDLGLSSIRLITNNPKKVVGLKGYGLYITERVREAEANLPTKFGTFRIIAYRDLLSNIVHVALVKGKIKGKKDILVRVHSECLTGDVFGSLRCDCGEQLHSALRMIEREGEGVVLYMKQEGRGIGLLNKIKAYRLQEKGFDTVEANEKLGFPPDLRDYGIGAQILVDLGLSSIRLITNNPKKVVGLKGYGLYITERVSQGAVERITEAKLPTKYGEFKMIGYANILNGEHHVALVKGEISDGESVLIRVHSECLTGDAFGSLRCDCGEQFAAAMQKIEEEGRGILLYMRQEGRGIGLINKIKAYELQDQGMDTVEANLALGFPADLRDYGIGAQILSDLGVKKAKLMTNNPKKIAGLTGYGIEIVDRQ",
    "response#3": "RVADARIPTRHGDFRAVGYSSVYDDVEHVALVKGDIAGEDGEGHDVLVRVHSECLTGDVFGSLRCDCGPQLDAAMEMVAAEGRGIILYMRGHEGRGIGLLHKLQAYQLQDAGADTVDANLELGLPADSRDYGLGAQILVDLGVTSMRLLTNNPAKRVGLDGYGLHIVDRVREAEANLPTKFGTFRIIAYRDLLSNIVHVALVKGKIKGKKDILVRVHSECLTGDVFGSLRCDCGEQLHSALRMIEREGEGVVLYMKQEGRGIGLLNKIKAYRLQEKGFDTVEANEKLGFPPDLRDYGIGAQILVDLGLSSIRLITNNPKKVVGLKGYGLYITERVGGRHGIEREAEANLPTKFGTFRIIAYRDLLSNIVHVALVKGKIKGKKDILVRVHSECLTGDVFGSLRCDCGEQLHSALRMIEREGEGVVLYMKQEGRGIGLLNKIKAYRLQEKGFDTVEANEKLGFPPDLRDYGIGAQILVDLGLSSIRLITNNPKKVVGLKGYGLYITERVGGRHGIED"
  },
  {
    "reference": "MTDRKNGLTYADAGVDIDAGNRLVDLIKPMVRATARPGADAEIGGFGGLFDLKAAGFKDPVLVAATDGVGTKVKIAIEAGLHAGIGIDLVAMSVNDLVVQGAEPLFFLDYFACGKLDPVATAAIVAGIAEGCRDSGCALIGGETAEMPGLYTDGDYDLAGFAVGAAERGTLLPGKDIAAGDAVIGLASSGVHSNGFSLVRKIVERSGLGYDANAPFSPVMTLGGALLAPTKLYVKSCLRAIRETGAVKGLAHITGGGFTDNIPRVLPPHLGVGIDLPRLPVLPVFKWLAEQGEIAELELLRTFNCGIGMIAIVRADAVEQVSDILTEAGETVCLLGEVIEAKGEHRVVYSGHLDLAW",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should be able to enhance the efficiency of 'de novo' IMP biosynthetic process. The designed protein sequence is ",
    "response#1": "CVGLDTSLDYIPEEFKSKFSNESDMLFAFNKEIIDATLDVSACFKVQIAYYEALGLKGLEAYKNTLSYLREKNALIIADIKRGDIAATAKMYAKAHFEGDFESDFITLNPYMGMDSIDPYLPYIEKNEKGVFVLVRTSNKGAQDIEYLEAGDGKKVYDVVGEKLNTLGKNYLGKHGYSSIGGVVGCTHQEEAKEMRDKLDTMPFLIPGYGAQGGTAKDVASYLKNGNGGIVNSSRKILLAE",
    "response#2": "MKRVLISVSNKEGIIPFARKLVHLGIEIISTGGTATLLREAEIDVMEVSELTGFPECLEGRVKTLHPVVHGGILADRSKDSHMKTLEELKIKPIDLVVINLYPFKETIQKKNVTLEEAIENIDIGGPTMLRAAAKNYRHVTVIV",
    "response#3": "CVGLDTSLDYIPEEFKSKFSNESDMLFAFNKEIIDATLDVSACFKVQIAYYEALGLKGLEAYKNTLSYLREKNALIIADIKRGDIAATAKMYAKAHFEGDFESDFITLNPYMGMDSIDPYLPYIEKNEKGVFVLVRTSNKGAQDIEYLEAGDGKKVYDVVGEKLNTLGKNYLGKHGYSSIGGVVGCTHQEEAKEMRDKLDTMPFLIPGYGAQGGTAKDVASYLKNGNGGIVNSSRKILLAA"
  },
  {
    "reference": "LCFEPKKKKEMSQKKKIALIALVSIVVPLCSAIPPPPPPQWDSSYSGLVLRPSCSLPTELHGRVQAALSTLRTKGCLLRDLVRVRDRDVFTSVSRALLGQPGHTYRYLDTRLFAGSPHEEEEEQEEQEEQEEQEEQEEQAAPPTPPGRCTVPPQFNVTLLNFMDPAAMRHLKEEPYYGMGKMAVGWHHDENLIAQSPVAVYSYKSCVGWKEEGEWDSKEVSRWRIGLKVAWDIYTPGLALPLQSGDCYYMRDDLNTTHQHCVLAGDNARFSSTHRVAECSKGTLEYIQSRCTEALANLGVDPESGAHSLRSLLPLTLKHCEDIHNEVEFEWLRQYWFQGQRYTRFCSWWSGPMELLERDWKLMETMVGPLHMAPHMAPHTASHTAPHTPTAPHLQLSCCYVTCHHGKTVKVRLLVESQSQAGFMRVECGARDYWLDTRLMGKSLHNGRGKTKRLVSLVHALGCQNAHNIRARNTIKHTDADREKDIPHRLCPHQTYFDIKQFIG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must be engineered to have a high affinity for Fe(2+) in order to increase its catalytic activity.2. We are looking for a protein with broad substrate specificity for 2-oxoglutarate + an N(6)-methyladenosine in mRNA + O2 = an   adenosine in mRNA + CO2 + formaldehyde + succinate.3. The protein contains novel Alpha-ketoglutarate-dependent dioxygenase FTO catalytic domains that confer a unique function or activity.4. The designed protein's functionality will benefit from Acidic residues compositional bias in its structure.5. The ideal protein would be one that plays a critical role in DNA dealkylation involved in DNA repair, regulation of multicellular organism growth, RNA repair, oxidative single-stranded DNA demethylation, oxidative single-stranded RNA demethylation through metal ion binding, oxidative DNA demethylase activity within cytoplasm, nuclear speck. The designed protein sequence is ",
    "response#1": "HPMFDEILARSFVGFVHENAAKLPKDLHAGMRLAFEKLRDKGYFQYDVVMAGGKHLSRTFVRRTLVGDFGITYKYLGLRIFAHAWNRPSSAPVFQSLYDLNQQMIARTKRSMVPAPPLCEYNLTLINYMEPDNPLLGLKDDPTYGMGKVSVSWHADSSLQDYSSIGVYHTLLPTKQKQPKTRCDWRIALRLSPEVPGADQTPPLVLPTDDGDAYYLNGDFNHFHQHMVLSGTAVRVSSTHRVAVGTLDYIQKRCAEALENLQTDPQTSTHSLRSLLPSTLQNTEEIHNEVEFEWLRQYWFQGRRFARFCSWWSKPMEQLEKDWKQMEVMTQLLLSIVEDESNVQEGRREMAETLLNALADRQQHRQTWRDRCQSSLAQSLPPEEAPVDRPHWNNEDPSMPLPFDLADIISRPPPQWDSSYSGLVLRPSCSLPTELHGRVQAALSTLRTKGCLLRDLVRVRDRDVFTSVSRALLGQPGHTYRYLDTRLFEAKAGSPRCCDPDMRAACQALWALNSFFCTDVPRREPEERLDLFIKAEGVVGKGSKHSDEWDLECTVPPQFNVTLLNFMDPAAMRHLKEEPYYGMGKMAVGWHHDENLIAQSPVAVYSYSCHSDAGEDLRGLEVSRWRIGLKVAWDIYTPGLALPLQSGDCYYMRDDLNTTHQHCVLAGDNARFSSTHRVAEGTLTYIQSRCEEALSNLRTDLETGSHSLLALLPTTLKHCEEIHNEVEFEWLRQYWFQGRRFARFCSWWTGPMEQLEKDWKLMETMTMLFLGTLEEEEGQEGEGRREMAEALLSALTDRHQQRQTWRDRCHSSLAQTLPPEEAPVDRPFWGVDDPSMPLPFDLADIINRV",
    "response#2": "QFNEILKRSYVGFVHEPGTTLPRDFHEGMRSAFEKLRDNEYFQYDVVMAGGKHLSRTFVRRTLVGDYGITYKYLGLRIFAHVWDKPHCAPVFKSLYQLNQRMIERTKRSMKPTPPLCDYNLTLINYMEPDNPMLGLKADPTYDMGKVSVSWHADSSLQDYSSIGVYHSLLGNKKKKAPCDWRIAMRLSPEVPGAATTPPLVLPTDGGDVYYLNGDFNHFHQHMVLAGTAVRVSSTHRVGTLTYIQSRCEEALSNLRTDLETGSHSLLALLPTTLKHCEEIHNEVEFEWLRQYWFQGRRFARFCSWWTGPMEQLEKDWKLMETMTMLFLGTLEEEEGQEGEGRREMAEALLSALTDRHQQRQTWRDRCHSSLAQTLPPEEAPVDRPFWGVDDPSMPLPFDLADIINRVP",
    "response#3": "FDEILARSFVGFVHENAAKLPKDLHAGMRLAFEKLRDKGYFQYDVVMAGGKHLSRTFVRRTLVGDFGITYKYLGLRIFAHAWNRPSSAPVFQSLYDLNQQMIARTKRSMVPAPPLCEYNLTLINYMEPDNPLLGLKDDPTYGMGKVSVSWHADSSLQDYSSIGVYHTLLPTKQKQPKTRCDWRIALRLSPEVPGADQTPPLVLPTDDGDAYYLNGDFNHFHQHMVLSGTAVRVSSTHRVHPQFNEILKRSYVGFVHEPGTTLPRDFHEGMRSAFEKLRDNEYFQYDVVMAGGKHLSRTFVRRTLVGDYGITYKYLGLRIFAHVWDKPHCAPVFKSLYQLNQRMIERTKRSMKPTPPLCDYNLTLINYMEPDNPMLGLKADPTYDMGKVSVSWHADSSLQDYSSIGVYHSLLGNKKKKAPCDWRIAMRLSPEVPGAATTPPLVLPTDGGDVYYLNGDFNHFHQHMVLAGTAVRVSSTHRVAVGTRDYIVNLCNSAIADALISGSHFDLMSVTKVQQALTEVECGWIRQFWAHGLRHARIQAFWKRVIESLELQWQQLESLTRQSLDSITRHMSEALHTATKSENARIATICDALLKAFEHRLRLREQWANFYSSPDFDHMENDLLPVRRPHFSASSPLPEALEPKIAQLHPMFDEILARSFVGFVHENAAKLPKDLHAGMRLAFEKLRDKGYFQYDVVMAGGKHLSRTFVRRTLVGDFGITYKYLGLRIFAHAWNRPSSAPVFQSLYDLNQQMIARTKRSMVPAPPLCEYNLTLINYMEPDNPLLGLKDDPTYGMGKVSVSWHADSSLQDYSSIGVYHTLLPTKQKQPKTRCDWRIALRLSPEVPGADQTPPLVLPTDDGDAYYLNGDFNHFHQHMVLSGTAVRVSSTHRVAV"
  },
  {
    "reference": "MKINGTPYRTIWLAKDGRTVEIIDQTRLPHEFVVVPLASLEDAAKSIKDMWVRGAPLIGATAAYGMALATLADASDAGLERAYQVLHATRPTAINLKWALDEMIAALTPLPVPERSAAAYARAAEICDEDSAQNAALGEHGAAIIAAHHKAKNRTINILTHCNAGWLATVDWGTALAPIYKAFDAGIPLHVWVDETRPRNQGASLTARELNWHGVPHTVIADNTGGHLMQHGMVDLCIVGTDRTTRNGDVCNKIGTYLKALAAKDNNVPFYVGLPSPTIDWNVADGVKEIPIEERSDREQTHIFGKTEDGVVREVQVTPDGSPAVNYGFDVTPARLVTGLITERGVCEASPEGLKGLFPERA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should consider the endogenous proteins and enzymes present in the L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 1/6 pathway to avoid interference with native metabolic processes.2. The protein design must be focused on enhancing the efficiency of L-methionine salvage from methylthioadenosine. The designed protein sequence is ",
    "response#1": "CREIGDGNLNYVFHLHDGKRGIIVKQALPYAKVVGESWPLSLNRATIESSALQIFAKYVPEFVPKVYGHDKELAVIVMEDLSSLTIVRKGFIEGEEYPLLSEHVGRFLGKVLFYTSDFGLNTEEKRVLDGKFINPDLCKITEDLVFTDPFGNYETNDYERELQSVIEELWCDKNLKLQVAQYKYKFLTRKEALIHGDLHTGSIFASSKETKIIDPEFATFGPLGFDLGQFIY",
    "response#2": "MRLRLNKSHVDGVNEGTVREGRVEVSFDLGQSWGTICGTYWSFREANVVCRQLNLGYAVSTAQSLTYGDSKRYPWKMVGTLCRGTEASLRDCFREKDYPKFCDSSNTKLAVVRCG",
    "response#3": "CREIGDGNLNYVFHLHDGKRGIIVKQALPYAKVVGESWPLSLNRATIESSALQIFAKYVPEFVPKVYGHDKELAVIVMEDLSSLTIVRKGFIEGEEYPLLSEHVGRFLGKVLFYTSDFGLNTEEKRVLDGKFINPDLCKITEDLVFTDPFGNYETNDYERELQSVIEELWCDKNLKLQVAQYKYKFLTRKEALIHGDLHTGSIFASSKETKIIDPEFATFGPLGFDLGQFIATYCL"
  },
  {
    "reference": "ANLLPPLQSWQCPRTPFTALRDFDVEYEVPSFSATGPVQAITTYEGGEDGSAVFVATRNRLYVLGPGLQPVESLATGPVGDPGCQTCAACGPGPHGLEGDTDAQVLVLEPALPALVSCIHGCCFLHELEPRRTALHLAPPVCLFSAHHNQPEECPDCVASPLGTVVTVVEQGHASYFYAASSLDETVAASFSPRSVSIRRLKADASGFAPGFAALSVLPQHLASYPIEYVYSFRAGAFVYFLTVQRASVTAAPGALHTRLGGQPYPVLRAAHTAPVGSQLATELSIMEGQEVLFGVFTARRDSSPGVSPNSVVCAFPIDLVDIVIEQGVKRCCESPAPQGMRRGLDFFQAHSFCPNPVIPSFNASCHHFPLLVSDSLLRVDLFNGLLGTVQVTALHVTRLENVTVAHMGTADGRILQVELARSLNYLLYVSNFSLGTNGQPVQRDVSRLGDHLFFASGDQVFQVPIQGPGCRHFLTCGSCLRAQRFTGCGWCGDVCGRQKECPGSWQQDHCPPELTEFYPQSGPRRGNTRLTLCGSNFYPHPLDLVHEGTHQVTVGQSPCRLLPKDNSNLRPVPRKDFVEELECEVGPLSTQPAGPANISLAVTNMPPGKHFRIEGASMLQGFSFMEPVLTDVKPVFGPRAGGTRLTLEGEGLSLGTSQAVLVNGTECPLERVSEGQLLCTTPPGATTASVPIRLQVGGAVVPGSWNFLYLEDPVVLGISPNCGYTGSRVTIHGQNLISAWSLVLSFHDGLRAVENRCEKQSPEQQSC",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be expressed specifically in the stress fiber The designed protein sequence is ",
    "response#1": "PLSLSDSLSVLQTNLYPPEKVRKEIGINYYSFNNNDTITPHTQSDYFYNTSNISSSKKQIKPRILIDNPSNFERFYCMNVSNFSKIVIILLKILLAATPVVKNYTGPINLIAEIVIDQSSPGSSASLVETMQSAIDFLRHKEIISKSTLGILLLIIKHSKFNQHLQFEYLSKIMYESNALVLLYKCLNHESVEKYLFSQNYLNSEEYFNEESLPSSSAASPPPPPIFQPLNCSGSSINSISSGGAGCEGGGANPPPVQLFENYKNLFSTITSLKLIQKVMKHHPSRISSLSASKSANILKKYCSINHPMIRLYSLKVIKNLVPYQTKKWKQINMRIISDC",
    "response#2": "SFVASPLLLEDCNKIASCDLPYSTFYANSQVIDKFSEYFAYVDQDPCHIRVLNANTDQSLILYTQSDVKNLHFGKNKHTKPHLLVMDALSNLYVWKILQKRSEISVSLLFTIRRANYGICDWIPNSSNMFSMIVKNSLYIVDISNIRSNNISPKVYAKDFNGLQAAKVDLPGPINSYTISSDRSVVAILVNSQIFFYSFPVSNLFSTNPSHRGNWAAIATMSLQLPSTANSISFLSSPPNNNDIIDKVICVSYANNTILGLFDFGLCAFTQEIEFSNEKEKSPIQQLLTFNNSNMIVAKRNQLLDIFIK",
    "response#3": "SFVASPLLLEDCNKIASCDLPYSTFYANSQVIDKFSEYFAYVDQDPCHIRVLNANTDQSLILYTQSDVKNLHFGKNKHTKPHLLVMDALSNLYVWKILQKRSEISVSLLFTIRRANYGICDWIPNSSNMFSMIVKNSLYIVDISNIRSNNISPKVYAKDFNGLQAAKVDLPGPINSYTISSDRSVVAILVNSQIFFYSFPVSNLFSTNPSHRGNWAAIATMSLQLPSTANSISFLSSPPNNNDIIDKVICVSYANNTILGLFDFGLCAFTQEIEFSNEKEKSPIQQLLTFNNSNMIVAKRNQLLDIFIK"
  },
  {
    "reference": "MEVPISGGGGGERFCHAAQVVGADGEMDGEAMARFAAGAGLLGRGLSYAVVSIVGPQGSGKSTLLNQLFGTSFTEMDALKGRSQTTKGIWIAKAVGIEPFTVVMDLEGTDGRERGEDDTAFEKQSALFALAVSDIVMINLWCHDIGREHAANRPLLKTIFEVLMRLFSPRKTTLLLVIRDKTKTPLEYLTQALKEDIQKIWNAVRKPEVYKEAALSEFFNVEVTALSSYEEKENLFKEQVGQLRQRFIHSIAPGGLAADRRGVIPASGFCLSALQIWKVIRENKDLNLPAHKIMVATVRCEEIADEKLRSFISDKGWLELETAANSGLVPGFGKKLNAILDFYLSEYDTEAMYFDEDVRTAKRQQLESEILKHTYDAFKKMLEHLHHVVLNKFKSDLEQSLRSGEGFAASARYCVQSSMAEFDAGLRDALVKHAEWDTTKVRSKLEQHIEAHATSVRGTKLAELKANYEKKLLDTLAGPVQSILETGEKDSWACIRRLYRHATESAILAFSASLSEFELDQTTIRKMVMELREHARSIVEEKAREEAGNVLMRMKERFSTVLSRDKDSMPRTWKGNEDIRAITREARLAALRLMSVMAAVRLDDKPDKIDRALTTALLDGGPLSQKRSIEFTSDPLASSTWEEVSEKNTLITPVQCKSIWRQFNAETEYAVAQAISMQEAHRRSNNWLPPAWTVLLLAILGYNEFIFLLRNPLYLLGLFVAFVVSYAAWLQYDITAYFRHGTLSGLLTITSGFLPTIMDIITAVINMS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should contain Cytoplasmic topological domains.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The designed protein should be involved in endoplasmic reticulum membrane fusion by localizing to endoplasmic reticulum membrane and exhibiting GTPase activity.4. The protein must exhibit the following characteristics: Probable GTP-binding protein that may be involved in cell development..5. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "ELGGSLGDLKEDTLENYDMMASRYNQSVYLQKRKTLEQKITDVYQDLVDQHGAHMVSKLSAKFASSLSSKKLPKDVSFALATEALRKDIVHQFLKNCSCITLNGSLDHAKHVTSFTRKLDSILSKQRFVELNSILAKSLKKVESAVAKAITQEISEPSESTWDRVLEKFKGAQDEYFYSKYETATGVDFGLGTSASVNERALEKFQFRAWSLLHLQMRKLISKDNLVIILKDRFEDKFRYDENGIPRLYQNSHELELNFTAAKEHALKALPILTLATLSDGTTIVPKYDVRDKRLQKKLGAAFDTTGEVDLKDEDVESDTDEEDEDENEPKCFAEAISETDKASVLSKFKKETDATFVESKRALIQHVTHIPYYIYIVILVLGWNEFMAVLRNPFFS",
    "response#2": "AVIYLSRSSKYTGNYVMHQTLSAYYYLMKPSLAPSKVMEVFTKAAEYMEIPVRRTDDEPLQKLFMSVRSELNLDLKNMKQEQAKFWKQHPAIVKTELLIQAQLTRESGVLSPALQGDFRRVLELAPRLLEELLKMAVIPRTAQGHGWLRPAVGVVELSQCIVQAVPLSARKSSGVSSEGISPFLQLPHFSDAVVKKIARKKVKSFQDLQEMRLEDRSELLTQVAGLSATDVEDIEKVLEMMPSITVDITCETEGEEGIQEGDIVTLQAWVTLKRPNGLVGALPHAPYFPFHKEENYWVLLADSVSNNVWFSQKVSFLDEGGAITAASKAISESMEGSGAGVKETNDAVREAIEKVKGGSRLVMGKLQAPAEGTYNLTCFCLCDTWIGCDKKQALKC",
    "response#3": "NYVMHQTLSAYYYLMKPSLAPSKVMEVFTKAAEYMEIPVRRTDDEPLQKLFMSVRSELNLDLKNMKQEQAKFWKQHPAIVKTELLIQAQLTRESGVLSPALQGDFRRVLELAPRLLEELLKMAVIPRTAQGHGWLRPAVGVVELSQCIVQAVPLSARKSSGVSSEGISPFLQLPHFSDAVVKKIARKKVKSFQDLQEMRLEDRSELLTQVAGLSATDVEDIEKVLEMMPSITVDITCETEGEEGIQEGDIVTLQAWVTLKRPNGLVGALPHAPYFPFHKEENYWVLLADSVSNNVWFSQKVSFLDEGGAITAASKAISESMEGSGAGVKETNDAVREAIEKVKGGSRLVMGKLQAPAEGTYNLTCFCLCDTWIGCDKKQAC"
  },
  {
    "reference": "MKIAIVGVTGLVGQVMQQVLTERKIQADEWILVASERSVGKQLTWEGKEYTLITNQEAVDAKPDIAIFSAGGSVSLEWAPKYADVGCFVIDNSSAWRMDPSKKLVVPEINGDQLTIEDKIIANPNCSTIQMVLALSGIHRKYGLNRLVISTYQSYTGTGSVAVHQYNTERANQEVKGEDRAYPHPIFANCIPHCDIFLDNDYTKEEMKLVNETQKILSDDSIKITATAVRVPVNGGHSEAVNVELTKPYEMSDMRELLAATPGVVVMDDLANNHYPTPLHAHHKDEVFVGRIRRDDTVKNGLNLWIVSDNLRKGAATNAVQIVEKIISRFF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be able to modulate 'de novo' L-methionine biosynthetic process in a way that leads to a desirable outcome.2. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..3. The substrate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "LNPLHKKYKIKRIVVSTYQSVTGTGKKAVDQLMGERNRALKGGNSEYPMAYKYPIDLNVIPQIDVFLDNGYTKEEMKMVKETNKIMRDDSIRVTATTVRIPVMGGHSESVNVEFENDFDLADVRKLLQDAPGVIVVDDPASQQYPMPVDAHDKDEVFVGRLRRDETQANTLNMWIVSDNLRR",
    "response#2": "LGGLFKEDLVEWVSSMTYQAASGAGAQNMVELLKQMGSLANATASTLEGNILEVDQKVNETMRASSFPTDNFGVPLAGSLIPWIDVGLENGQSKEEWKAQAEANKLLNTKSIIPMDGTCVRIGAMRCHSQGLTIKLKKDVDLKNIEEIISSANEWVKLIPNERSVTAKELSPTTVSGTLSIPVGRVRKMSLGSQYLNAFTVGDQLLVR",
    "response#3": "LNPLHKKYKIKRIVVSTYQSVTGTGKKAVDQLMGERNRALKGGNSEYPMAYKYPIDLNVIPQIDVFLDNGYTKEEMKMVKETNKIMRDDSIRVTATTVRIPVMGGHSESVNVEFENDFDLADVRKLLQDAPGVIVVDDPASQQYPMPVDAHDKDEVFVGRLRRDETQANTLNMWIVSDNLRY"
  },
  {
    "reference": "MTTTLQPPTTSGSRREDGEGSVQVHQDASMRIEEGALVIAVYGKGGIGKSTTSSNLSAAFSKLGKRVLQIGCDPKHDSTFTLTKKMVPTVIDILETVDFHTEELRPEDFVFEGFNGVMCVESGGPPAGTGCGGYVTGQTVKLLKEHHLLEDTDVVIFDVLGDVVCGGFAAPLQHAHYCLIVTANDFDSIFAMNRIMQAINAKAKNYKVRLGGVIANRSEDTDQIDKFNERTGLRTMAHFKAVDAIRRSRLKKCTIFEMEPTPEVEAVQQEYLNLARKMLEDVEPLEAESMKDRDIFDLLGFD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein should be able to manipulate the activity of enzymes in the Porphyrin-containing compound metabolism, chlorophyll biosynthesis (light-independent) pathway, either by activating or inhibiting their function.2. The protein must exhibit the following characteristics: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein..3. The designed protein should have a high specificity for ATP, [4Fe-4S] cluster, Mg(2+) binding, with minimal off-target effects.4. The protein must bind to [4Fe-4S] cluster in order to perform its enzymatic function.5. Target a Polar residues compositional bias in the protein's composition for improved properties.6. A protein with carbonyl sulfide nitrogenase activity, 4 iron, 4 sulfur cluster binding, ATP binding, nitrogenase activity is needed to enhance photosynthesis, dark reaction, light-independent chlorophyll biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "ALHGVSRVTNSMKGVHTVYHAPQGDDYITATYTMLERTPEFPKLSISVVRGQDLARGTSRLPGTVEQVAKHYNPELIVVAPSCSTALLQEDLGQMARASGIDENKIMVYAVNPFRVAETEAAEGLFTELVKRFAAEQPKTEKPSVNLLGFTSLGFHLRSNLTSLRRMLKTLGIEVNVVAPWGAGIDDLKKLPAAWVNIAPFRELGCQAAGYLKENFGMPSITEAPLGVNATLRWLRAIIAEVNKIGAEKGMAPIEMPEMRDFSLDGQSAPSGVPWFARTADMESFSNKRAFVFGDATQVVGVTKFLKDELGMKIIGAGTYLPKQADWVREQLEGYLPGELMVTDKFQEVSAFIEEEMPELVCGTQMERHSCRKLDVPCMVISTPTHIENHLLGYYPFFGFDGADVMADRVYTGNWLVNVEKGTSCPISCVSWLYQKIEDSFFLVIGTKTCGYFLQNAMGVMIFAEPRYAMAELEEGDISAKLNDYGELRRLCLQIKKDRNPSVIFWIGTCTTEIIKMDLEGIAPKLEAEIRVPIVVARANGLDYAFTQGEDTVLAAMAQRCPSHHYIENTNPHVPLVLFGSLPDPVVTQLVMELKKQGIIVSGWLPSKRYTELPVIQEGYYVAGVNPFLSRTATTLMRRRKTKLIGAPFPIGPDGTRAWIEKICSVFSIKPEGLAEREKDIWDSLEDYIALIRGKSVFFMGDNLLEVSLARFLTRCGMTVYEIGIPYMDKRYQAAELALLKSTCDDMNVMMPTIVEKPDNYNQLDRIRDLKPDLVITGMAHANPLEARGINTKWSVEFTFAQIHGFTNARDILELVT",
    "response#2": "ALHGVSRVTNSMKGVHTVYHAPQGDDYITATYTMLERTPEFPKLSISVVRGQDLARGTSRLPGTVEQVAKHYNPELIVVAPSCSTALLQEDLGQMARASGIDENKIMVYAVNPFRVAETEAAEGLFTELVKRFAAEQPKTEKPSVNLLGFTSLGFHLRSNLTSLRRMLKTLGIEVNVVAPWGAGIDDLKKLPAAWVNIAPFRELGCQAAGYLKENFGMPSITEAPLGVNATLRWLRAIIAEVNKIGAEKGMAPIEMPEMRDFSLDGQSAPSGVPWFARTADMESFSNKRAFVFGDATQVVGVTKFLKDELGMKIIGAGTYLPKQADWVREQLEGYLPGELMVTDKFQEVSAFIEEEMPELVCGTQMERHSCRKLDVPCMVISTPTHIENHLLGYYPFFGFDGADVMADRVYTWTEGGNGDVTMQPHVGAMRIAASMHGVHYVLHAPQGDTYADLLFTMIERRGQRPPVTYTTFQARDLGGDTAELVKRHVQEAADRFQPDALLVGESCTAELIQDQPGALAQGMGLPMPVVTLELPAYSKKENWGASETFYQLMRGLLKQSVPPQPSHDVQAWKHEGRRPRVNLLGPSLLGFRCRDDVLEVQRLLSLHGIDVGVVAPLGAGVEDILRLPQADLNVCLYPEVAESSCSWLERNFGIPFSKTVPIGMGATHDFLVEVHALLGMTPPEAAEGYQRSRMPWYSESVDSTYLTGKRVFIFGDGTHAIAAARICSEELGFTVVGLGSYSREMARPVRAAAKKLGLEALICDDYLAVEAAMAEAAPELVLGTQMERHSAKRLGIPCAVISTPMHVQDVPARLSPQMGWEGANVIFDAWVHP",
    "response#3": "ALHGISRITNSMKGVHTVYHAPQGDDYITATYSMLERTPDFPGLSISVVRGRDLAQGVSRLPGTLQQVAHHYSPDLTVIAPSCSTALLQEDLHQLAAHSGVPEEKLLVYALNPFRVSENEAADGLFFELVKRFAVAQEKTPRPSVNLLGFTSLGFHLRANLTSLRRMLQTLGVSVNVVAPWGAGIEDLRKLPAAWLNIAPYREIGETAAGYLGETFGMPAIHEAPIGVEPTLAWLRSVIEKINAVGVEQGVPPIGMPKLNAFSLDGMSAPSGVPWFARTADMESFSNKRAFVFGDATHTVSIVKFLRDELGMKIIGAGTYMHRHADFVRRELEGYLSGELLVTDKFQDVSKVIEDEMPDLVCGTQMERHSCRKLDVPCMVICPPTHIENHLLGYYPFFGFDGADVIADRVISTAEPSIWTALHGVSRVTNSMKGVHTVYHAPQGDDYITATYTMLERTPEFPKLSISVVRGQDLARGTSRLPGTVEQVAKHYNPELIVVAPSCSTALLQEDLGQMARASGIDENKIMVYAVNPFRVAETEAAEGLFTELVKRFAAEQPKTEKPSVNLLGFTSLGFHLRSNLTSLRRMLKTLGIEVNVVAPWGAGIDDLKKLPAAWVNIAPFRELGCQAAGYLKENFGMPSITEAPLGVNATLRWLRAIIAEVNKIGAEKGMAPIEMPEMRDFSLDGQSAPSGVPWFARTADMESFSNKRAFVFGDATQVVGVTKFLKDELGMKIIGAGTYLPKQADWVREQLEGYLPGELMVTDKFQEVSAFIEEEMPELVCGTQMERHSCRKLDVPCMVISTPTHIENHLLGYYPFFGFDGADVMADRVYT"
  },
  {
    "reference": "MKLSTLSTQVENLANARVLCVGDIMLDRFVYGSVERTSPEAPVPVLGIINEKKMLGGAGNVARNIVSLGAKATLISVVGDDPVGRELTSMVGKENGIDPCLLVESGRVSTEKTRFVAGNQQLLRADNEMISPISELVSGNLLRVAKDLIXESDVLIISDYAKGIINENICQSLITEARKSGKRIIVDPKGSNFSQYRDSNIITPNRNELHAATGLNVNTSEEIISAATKLMEKFNLDHTFITRSSEGISLVSRDGKVEHLNAQAREVYDVSGAGDTVVAALAVALGANISLSDSARLANAAAGVVVGKAGTAVAETSELVHALRASDLTDAEAKIVPLKNAIQTINSWRKDGDTIGFTNGCFDLLHPGHISLLCQAHAKCDRLIVGLNSDNSIKKLKGKXRPIQGEDARAQVLASLETVXLVIVFGEDTPLNLIQNILPEILVKGSDYTEEEVIGADIIRKNGGEILLTPLEPGYSTTNTISRLKE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be able to bind ATP ligand in a variety of conditions2. The protein design should take into account the physiological conditions of the ADP-L-glycero-beta-D-manno- heptose biosynthesis, ADP-L-glycero-beta-D-manno-heptose from D- glycero-beta-D-manno-heptose 7-phosphate: step 3/4 pathway.3. The designed protein is the optimization of the active site to achieve high catalytic efficiency for ATP + D-glycero-beta-D-manno-heptose 7-phosphate = ADP + D-   glycero-beta-D-manno-heptose 1,7-bisphosphate + H(+).4. The designed protein should have ATP binding, phosphotransferase activity, alcohol group as acceptor, heptose-1-phosphate adenylyltransferase activity, heptose 7-phosphate kinase activity to facilitate lipopolysaccharide core region biosynthetic process, ADP-L-glycero-beta-D-manno-heptose biosynthetic process.5. The protein contains novel Carbohydrate kinase PfkB domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MPAFQEARLLVIGDVMLDRYWHGAASGVSPEAPVPVVRVGNEENRPGGAGNVALNIAALGAAARLIGLVGNDETGRELNSRLNAAGVYCDFIESNDKPTITKLRVISQHQQLIRLDFEQLFDKPDVGDLFEKAKALIDDTQVLVLSDYAKGALQGVEQLIELGRNRGIPIIVDPKGSCFEKYRGATLITPNLIEFEKVVGKSQNEEELVNKGLKLLSELSLEALLITRGEHGMTLIRPHSPELHLPARAQEVFDVTGAGDTVVGVLSAAIAVGENLADATALANLAAGLVVGKLGTAAISGPELRRALHGDAEHHAGAVVDEAQLASEWRRQDLRVGFTNGCFDILHPGHVKVLTAARGACDRLIVGLNSDASVKRLKGEGRPVQDERARAEVLAALEAVDLVAIFEEGTPIELITKIKPSVLVKGGDYTREQVVGHEIVEACGGEVVLIDILPGHSTTSLVDRART",
    "response#2": "MSRVLVVGDLMIDHYIWGSCDRISPEAPVQVVNIKNETKRLGGCGNVVSNLIALGAEVGVISVVGDDELGGEILKLLKDRGAKAELVIAQKGRKSSQKSRVMVTHQQVLRLDTESTSDIDCADEIVGKFQGILNSYDIVLLSDYGKGVLSDYLTKQIISITKNSAKMVLVDPKGKDYSKYKGATLLTPNKKEASEALGFVIDSDEKLELALKTMKMNYELEYSLITLSEDGIGLLAQKMVKFPALAKEVFDVTGAGDSVLATLGYCLASKMSIEDAIDNANLAAAVVVGKVGSALMEGSHYVTDHAELLATIKDVERQRGRTIVFTNGVFDILHAGHVHLLRQAKQLGDVLVVGVNSDQSAQRLKGKHRPINSERDRLALVAALDPVDYVALFDEDTPATLIRTLRPHIHVKGGDYADEQLPEAGAVHEVGGRIVIIPLVGSMSTSSVIDRIKDKA",
    "response#3": "MSRVLVVGDLMIDHYIWGSCDRISPEAPVQVVNIKNETKRLGGCGNVVSNLIALGAEVGVISVVGDDELGGEILKLLKDRGAKAELVIAQKGRKSSQKSRVMVTHQQVLRLDTESTSDIDCADEIVGKFQGILNSYDIVLLSDYGKGVLSDYLTKQIISITKNSAKMVLVDPKGKDYSKYKGATLLTPNKKEASEALGFVIDSDEKLELALKTMKMNYELEYSLITLSEDGIGLLAQKMVKFPALAKEVFDVTGAGDSVLATLGYCLASKMSIEDAIDNANLAAAVVVGKVGSALGLGIESLLVEDEDRLLVIGDVMLDRYWHGAASGVSPEAPVPVVRVGNEENRPGGAGNVALNIAALGAAARLIGLVGNDETGRELNSRLNAAGVYCDFIESNDKPTITKLRVISQHQQLIRLDFEQLFDKPDVGDLFEKAKALIDDTQVLVLSDYAKGALQGVEQLIELGRNRGIPIIVDPKGSCFEKYRGATLITPNLIEFEKVVGKSQNEEELVNKGLKLLSELSLEALLITRGEHGMTLIRPHSPELHLPARAQEVFDVTGAGDTVVGVLSAAIAVGENLADATALANLAAGLVVGKLGTAAISGPELRRRIGLKVVFTNGCFDILHRGHIEYLGFCRQQGDLLVVGLNSDRSVRQIKGPDRPINTQQDRAALLAALASVDFVTIFDEPTPMALIEQIRPDVLVKGRDWQEKGVVGAEFVRSYGGKVVFAPLLEGRSTTATIEQILTRG"
  },
  {
    "reference": "MPEPAKSAPKKGSTRTAAKGGKKRRKSRK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have a specific function within the nucleus.2. The protein must exhibit the following characteristics: DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template; Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability; Core component of nucleosome. The designed protein sequence is ",
    "response#1": "GEEKSAEKAPAGKKPKAEKRLPASKASSKEGGAGDKKGRKKAKKSVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAQEAARLARYNKKPTITSREIQTSVRR",
    "response#2": "GEEKSAEKAPAGKKPKAEKRLPASKASSKEGGAGDKKGRKKAKKSVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAQEAARLARYNKKPTITSREIQTSVRR",
    "response#3": "GEEKSAEKAPAGKKPKAEKRLPASKASSKEGGAGDKKGRKKAKKSVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAQEAARLARYNKKPTITSREIQTSVRR"
  },
  {
    "reference": "MKIGIIGAMEQEVAILKEQINGLATTIKAGCTFHTGTLNGAEVVLLQSGIGKVAAAVGTTLLISDHNVDVVLNTGSAGGFDSSLNLGDVVISTEVRHHDADVTAFGYEMGQMAQQPAAFKADEKLMAVAEKALTQMEDKHAVRGLICTGDAFVCTPERQAFIRSHFPSVIAVEMEASAIAQTCHQFSVPFVVVRAISDVADKESPMSFEEFLPLAAESSSEMVMKMVTLLK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must be able to adenosylhomocysteine nucleosidase activity. The designed protein sequence is ",
    "response#1": "ESPLHIHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWCAEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITALR",
    "response#2": "IHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWCAEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITAL",
    "response#3": "IHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWCAEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITAL"
  },
  {
    "reference": "MNNCRQNLENFDPEVFGYLNDEIKRQEEHIELIASENFVSKAVLETMGTELTNKYAEGYPGKRYYGGCEHVDKIEQLAIDRLKKLFNADHANVQPHCGANANIAVYVAVLKPGDTVLGMRLTEGGHLTHGSPVNMSGKFYNFVDYGVDPETGTIDYENVRELALKHKPKLIVAGASAYPRIIDFKKFREIADEVGAYLMVDMAHIAGLVATGDHPSPVPYADFVTTTTHKTLRGPRGGAILCKEEHKKLLDKSVFPGFQGGPLEHIIAAKAVCFKEDLQPEFKEYTHQILKNAKAMEKVFLDNDVRLVSGGTDNHLLLIDCRSFGMTGKEAENVLSEVNITTNKNTIPNDPETPFVTSGIRIGTPAITTRGLKEAEATKVAEFMIDALKKRRPAEEIKNDVVELMKQFPINR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.2. Ideal protein should have a binding affinity for key enzymes in the Amino-acid biosynthesis pathway to enhance the overall efficiency of the pathway.3. The protein must have high specificity towards pyridoxal phosphate binding, glycine hydroxymethyltransferase activity.4. The protein requires pyridoxal 5'-phosphate to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "AQVDPPVAAAIAAEAHRQRNNIELIASENFASPAVMEAQGSVLTNKYAEGYPGRRWYGGCENVDVIEQLAIDRAKAIFGAEHANVQPHSGSQANTAVYFSVLQPGDKILTMDLAHGGHLTHGHKANFSGRFYAVTHYGVSPEDERINYAELEATARALKPALITVGASAYSRVIDFERMGRLAREIGAYLFVDMAHIAGLVAAGVHPSPVPHADFVTSTTHKSLRGPRGGIILCKEEFAKRIDSQVFPGIQGGPLMHAIAGKAVCFTEALLPEFKTYQQQVVRNAQAIAARLTQHGYRIVSGGTDNHLMLVDLRSMGLNGTLASHALDLAGITVNKNSIPFDTGSPMKPSGIRIGTPAVTTRGMREADVERVADFIAGALDAPADTERLAALRTRVAAFPLYP",
    "response#2": "AQVDPPVAAAIAAEAHRQRNNIELIASENFASPAVMEAQGSVLTNKYAEGYPGRRWYGGCENVDVIEQLAIDRAKAIFGAEHANVQPHSGSQANTAVYFSVLQPGDKILTMDLAHGGHLTHGHKANFSGRFYAVTHYGVSPEDERINYAELEATARALKPALITVGASAYSRVIDFERMGRLAREIGAYLFVDMAHIAGLVAAGVHPSPVPHADFVTSTTHKSLRGPRGGIILCKEEFAKRIDSQVFPGIQGGPLMHAIAGKAVCFTEALLPEFKTYQQQVVRNAQAIAARLTQHGYRIVSGGTDNHLMLVDLRSMGLNGTLASHALDLAGITVNKNSIPFDTGSPMKPSGIRIGTPAVTTRGMREADVERVADFIAGVLASLDETSTDEIVARVRARVAEFPLPY",
    "response#3": "MAKGLFESLIRGNKDIQVDSAGIGAISGLPPSAHAIEVMAEIGIDISHIRSKPVNSELIRKADFIFCMSYSHLDSLLLLYPEAGEKTFLLLEFANDLPIMAREIPDPIGGSIELYKLCRDQMRQVMPA"
  },
  {
    "reference": "MLQPKRTKFRKMHKGRNRGLAQGTDVSFGEFGLKACGRCRLTARQIEAARRAMTRAIKRQGKVWIRVFPDKPITEKPLEVRMGKGKGNVEYWVALIQPGKVLFEMAGVPEETAREAFKLAAAKLPVGTTFVTKTVM",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design must be able to perform structural constituent of ribosome in order to contribute to translation. The designed protein sequence is ",
    "response#1": "MI",
    "response#2": "MR",
    "response#3": "MIGR"
  },
  {
    "reference": "MQDQSSVFVTIPEAIEDLRAGKMIIVVDDEDRENEGDFIMAADKITPEAVNFMAKYGRGMICVPTTGERLEELGLPMMVERNSALLGTAFTVTVDAVQGTTTGISAHDRALTIKCFADAGAKPEDLARPGHIFPLRAEDGGVLVRAGHTEAVVDLVRLAGLGSSGVLCEILSEDGSMARLPELTQIAANWDLKIVTIADLIKYRRVTERLVFKFASCRIPTQYGDFTAHGYQATVESSPYIALVMGDVSDGEPTLVRIHSGCLTGDVLGSLRCDCGEQLHGAMRAIADEGKGVLLYIYHQEGRGIGLVNKLKAYVLQDHGADTVEANHMLGLPGDMRDYGLGAQILVDLGLKKIRIMTNNPGKLAGLQGYDLEIVERVPLVCEPNADNARYLNTKREKMGHELDEQLFQDHCDNCSNE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..2. Ideal protein should have a binding affinity for key enzymes in the riboflavin biosynthesis pathway to enhance the overall efficiency of the pathway.3. The Nucleophile; for GTP cyclohydrolase activity active site of the designed protein must be optimized for substrate binding and specificity.4. The protein must be able to manganese ion binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity.5. The protein should have a high affinity for GTP, Mg(2+). The designed protein sequence is ",
    "response#1": "RLAETVLPTAIAGEFKAIVYESEVDPNHHLALVKGEIDSEQPILVRVHSQCLTGDVFGSLRCDCGSQLHTAMKMIEKEGKGVLVYMRQEGRGIGFANKMKAYALQDAGHDTVEANLALGFKPDLRNYGIGAQILRDLGVRKMRLMTNNPKKIVGLEGYGLEVVERIALGASAKLRGERIATTRLPTMHGDFRAYGYRDLVGGGEHLALVKGELSGDDPVLARIHSECLTGDAFGSLRCDCGPQLQAALAAVAKAGRGVVVYLRGHEGRGIGLAHKLQAYELQDNGHDTVDANIELGLPADARDYGVGAQILADLGVRSLALMTNNPAKLTGIEGYGLDVAERREAEAKLPSAFGDFRIVAYTNELDGKEHVAIVKGEIDSSKPVLVRVHSECLTGDIFGSHRCDCGPQLHAALRMIEAEGRGIVLYMRQEGRGIGLINKLKAYRLQEQGYDTVEANKKLGFPADLRDYGIGAQILLDLGVKQMRLMTNNPRKIRGLSGYGMQVAERVDG",
    "response#2": "GVDMPTAWGDFELIAYEQTNTQEIHMALIKGKWEKDEKVLVRVHSSCVTGDIFGSCRCDCGPQLHRAMEMVQEAGKGVVLYMNQEGRGIGLVNKLKAYKLQEEGMDTVQANLALGFPMDKRDYGVGAQILRDLGISKIRLISNNPTKRAGLLGYGLEIVDTVGHVVVH",
    "response#3": "GVDMPTAWGDFELIAYEQTNTQEIHMALIKGKWEKDEKVLVRVHSSCVTGDIFGSCRCDCGPQLHRAMEMVQEAGKGVVLYMNQEGRGIGLVNKLKAYKLQEEGMDTVQANLALGFPMDKRDYGVGAQILRDLGISKIRLISNNPTKRAGLLGYGLEIVDTVGYAK"
  },
  {
    "reference": "MSLEDKELVLGVTGGVACYKALDLVRRLKEKGARISVIMTSSAQEFIQPLAFQATSGRPVATSLFSLEGEEKIGHINIVENIDGLIVAPATANILGKVANGIADDYLSTSMLALKAPLFLAPAMNHNMWDHPAVQQNLQTLKDRNANIIPPEYGYLACGSYGTGRMAAVEKIVANLEEFFTVKAAPLQGVSVLVTAGPTREKIDAVRYLSNYSSGKMGYAIANYCRQLGAKVTLISGPTALPEPEGMQFIRVSSAAEMHQAVMQHATAAQLIIKSAAVADYRIEKPNVQKTKKKEQLTLNLTLNRDILKELGQKKGDKQFLVGFAAESQNLEGYARQKLEAKNLDLIVANNILEKDAGFDADSNRVLLIDQQNTHPLSLQPKEEIAKQIIQYVLKHQKWISMREV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein with phosphopantothenoylcysteine decarboxylase activity, FMN binding, phosphopantothenate--cysteine ligase activity, metal ion binding functions is required to enable pantothenate catabolic process, coenzyme A biosynthetic process.2. The protein contains novel DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains that confer a unique function or activity.3. The protein requires Mg(2+) to activate a critical catalytic site.4. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..5. Ideal protein should have a binding affinity for key enzymes in the Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis pathway to enhance the overall efficiency of the pathway.6. The CTP binding site should be located in a region of the protein that is accessible to the ligand.7. The Proton donor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "KKIVLGVTAGIAAYKTTFLVRLFVKAGAEVKVVMTPSAKDFVTPLTLSTLSKNEVHTSFTNEDDENAQWNNHVELALWADLFVIAPATANTLSKMAHGTCDNLLLAVYLSAKCPVYYAPAMDLDMYQHPSTKATFTTLASFGNIQIPATHGELASGLVGQGRMAEPEAIVAFIEAELAPGLPLAGQRVVITAGPTREPLDPVRYISNHSSGKMGYALARAAVEAGASVQLVSGPVALAVPDRVHCHPITTAREMLDVCMDLVENCDIFIGCAAVADYRPMVESEQKIKKSNEEMSLQLTRNPDILASVAGHSAKPFSVGFAAETEKLREHALGKLDRKGLNMIVANDVSDSSIGFNSENNAALLFWRDGREELPLMAKPSLARHLVDRIVPLLTAR",
    "response#2": "KKIVLGVTAGIAAYKTTFLVRLFVKAGAEVKVVMTPSAKDFVTPLTLSTLSKNEVHTSFTNEDDENAQWNNHVELALWADLFVIAPATANTLSKMAHGTCDNLLLAVYLSAKCPVYYAPAMDLDMYQHPSTKATFTTLASFGNIQIPATHGELASGLVGQGRMAEPEAIVAFIESVLLGDRAGLKVVITAGPTVEDLDPVRFITNRSSGKMGFALSAAAKALGAQVVLIAGPVKLDTPEGITRIDVRSALEMLHAVEDNLKGCDVFIGCAAVADYRADHIETQKISKRDHSDGLTLKLVRNPDIVATVGKLENNRPFTVGFAAETENGEDHAKRKLRDKNLDLIALNYVQHHHIGFDSDQNELAVFWSGGETQIERRSKEELAERLVALIAERYFAASR",
    "response#3": "KKIVLGVTAGIAAYKTTFLVRLFVKAGAEVKVVMTPSAKDFVTPLTLSTLSKNEVHTSFTNEDDENAQWNNHVELALWADLFVIAPATANTLSKMAHGTCDNLLLAVYLSAKCPVYYAPAMDLDMYQHPSTKATFTTLASFGNIQIPATHGELASGLVGQGRMAEPEAIVAFIEARLGSALAGRKAVVTAGPTFEPIDPVRGLTNRSSGKQGYAIAAALVELGAEVTLVSGPTALGAPVGVTRIDAESALEMQAATQNALPADIAVLSAAVADWRVDTIAGGKIKKGPGGPPTLALIENPDILAGIAAPGPNRPALVIGFAAETTDLEANARAKLSRKGCDWIIGNDVSDDVFGSDGNAVLLWRDGRAEPLPPLSKARLARQLVALIAERLDAAA"
  },
  {
    "reference": "MKVGVVGATGQVGAVMRRLLAERSFPVDEIRFFASARSAGTTLPWEGVDVRVEDAEVADPRGLDVALFSAGGATSKELSPRFAAAGVTVIDNSSAWRMDPDVPLVVAEVNGAEAHHAPKGIIANPNCTTMAAMPVLKPLHDEAGLVRLIVSTYQAVSGAGLAGVDELDKQVHAGVDRGAELTHDGAAVDLGQPAKFVRPIAFNVLPLAGKIVDDGTLETDEEQKLRNESRKILAIPHLLVSGTCVRVPVFTGHSLSINAEFARPISVGRATELLRDAPGVELCDVPTPLQAAGRDPSYVGRIRQDRTVDGGRGLALFVSNDNLRKGAALNAVQIAELLLNR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The NADP(+), phosphate binding site should be located in a region of the protein that is accessible to the ligand.2. The protein contains novel Semialdehyde dehydrogenase NAD-binding domains that confer a unique function or activity.3. The Acyl-thioester intermediate, Proton acceptor of the protein must be engineered to enable effective catalysis of L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.4. The designed protein should have NAD binding, NADP binding, aspartate-semialdehyde dehydrogenase activity, protein dimerization activity to facilitate 'de novo' L-methionine biosynthetic process, isoleucine biosynthetic process, threonine biosynthetic process, diaminopimelate biosynthetic process, lysine biosynthetic process via diaminopimelate.5. A protein that is capable of catalyzing reactions within the L-threonine biosynthesis, L-threonine from L-aspartate: step 2/5, Amino-acid biosynthesis pathway with high efficiency and specificity.6. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.. The designed protein sequence is ",
    "response#1": "MTCVAILGATGAVGRELLELLESRNFPVSELKLLASERSAGQKLTFKGEDLLVEPVSDRAFENVDIVLASAGGSTSKTWAPIATEKGAIVIDNSSAFRMKEEVPLVVPEVNPQAAANFAKRNIIANPNCSTAQMVVALKPLHDLATIKRVVVATYQSVSGAGKEGVDELWDQTKGVFVNDKPEPRKFTKQIAFNVIPHIDVFMEDGYTKEEWKMVAETKKILDPKIKLTATCVRVPVFVGHSEAVNLEFANPISADEAREALREAPGISVIDKREAGGYATPVEAAGEYDTYVSRIREDVTVENGLSLWVVSDNLRKGAALNSIQIAETLVRNYL",
    "response#2": "MTKVGILGATGAVGREMMKILAERQFPISELRLFASSRSAGKVVEWNGRPVMIQEPSEENFRGLDIVLGAAESDIARLYAPEVVRAGAVFVDNSSAYRMDDDVPLVIPEVNPEDVKDAYAAQKGGAKKVIIAAPASNEDITVVLGVNAEKYDPKKNTIISNVSGTCVRVPVFTGHLKPIQDAVGIERINVSTYQSVSGAGKAGIDELAGQTARLLNGVPAESEHFPQQIAFNCIPQIDQMMENGYTREEMKMVWETQKIFNDPGITVNPTCVRVPVFYGHAESVHVETCAPIDAQDVIQLLENAEGVEVFQGEEFPTQVRDSGGKDHVMVGRIRNDISHHSGVNLWVVADNVRYDNEMGYASRMCDLLEYIAK",
    "response#3": "MTKLAIVGATGVVGRTALKVLEEKNLPISEYVLFASNKSAGKKVKFMNKEYVVKELTEHSFDEGFDFAIFSAGGETSRKYAPIAASKGCIVVDNSSAFRMDKDVPLVVPEVNPEEIKNPPKGIIANPNCTTMAAMPVLKPLHDLATIKRVVVATYQSVSGAGKEGVDELWDQTKGVFVNDKPEPRKFTKQIAFNVIPHIDVFMEDGYTKEEWKMVAETKKILDPKIKLTATCVRVPVFVGHSEAVNLEFANPISADEAREALREAPGISVIDKREAGGYATPVEAAGEYDTYVSRIREDVTVENGLSLWVVSDNLRKGAALNAVQIAEVIDAEATLERLIVSTYQAVSGSGLAGADELLGQVRAALEGDTAGLVHDGGAVQMPEPAVYVRPIAFDVIPLAGSIVDDGLDETDEEKKLRNESRKILERPDLRVSGTCVRVPVFTGHSLSINAEFADALTAARAKELLADAPGVVLTDVPTPLQAAGSDPSYVGRIRQDEGVPGDRGIALFISNDNLRKG"
  },
  {
    "reference": "MAVLGNYCDPRTSLAQAWVQGGFSPCFYATLVPTVLLTFSLFFGTFHWVCYQHYGTAMEPKFVPRSRLYRLQQGLSVLLLLQFLVGMIWRAEQGTELHGHVLVYGCLSALGWACAIALLHLERRRVLVQDRTRGHSAVLLLFWALAFSAENLAFVSWYSPLWWWGLESTDQQVEFALWMVRYLGTGALFLLGLKAPGLPKRPYMLLINEDERDLEHGQLLLGGSPQSQSTWQGFRKKFGLLLPYMWPRGSVFLQALVLFCVCLLGVERAINVFVPIYYKNIVNELSDGSSWKTLTTTVCIYVLLKFLQGGGAGASGFVSNLRSFLWIRVQQYSSRVVQVRLFAHLHSLSLRWHLGRRTGDVLRSVDRGASSIDSLLSYIAFSIFPTIADIVIAIIYFISYFSAWFGLIVFVCMFLYLTLTVIITEWRTKYRRDMNAQDNAAKSKAVDSLLNFETVKYYNAESYEVGCFEEAVLKYQSSEWKTNASLALLNQTQNVIIGAGLLAGSLLCAYFVSEKKFQVGDYILFATYIIQLYTPLNWFGTYYRVIQRSFIDMESMFKLFEEEEEVKDDINAGNLQFKAGKVEFENVHFSYVDGKEILKDISFTVMPGQTVALVGQSGSGKSTIIRLLFRFYSVQGGCIRIDGQDISKVRLLMGSAAWKMMVGRAAADAVVLILLIHHHLAGYDTQVGERGLKLSGGEKQRVAIARTILKAPQIILLDEATSSLDTKTERNIQASLAKVCANRTTIVVAHRLSTIIAADQILVLHDGA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a specific function within the Golgi membrane. The designed protein sequence is ",
    "response#1": "GSPSLDYFETCSNFVPRAGIPTFSPYAVIKNFDEVNRMYYIQVVGNLSGVITIVGGNGSHIHAASVYSETYALGKLVTSNTTKLCDVVMDPHSFEVPIKCPWAAGSAAFMVKVPFKSTLSDYSKYELTTLTTRVIVVDGTPEKNVITCMKFGLSTTLYYR",
    "response#2": "GSPSLDYFETCSNFVPRAGIPTFSPYAVIKNFDEVNRMYYIQVVGNLSGVITIVGGNGSHIHAASVYSETYALGKLVTSNTTKLCDVVMDPHSFEVPIKCPWAAGSAAFMVKVPFKSTLSDYSKYELTTLTTRVIVVDGTPEKNVITCMKFGLSTTLYYR",
    "response#3": "GFNAALEVLTTKELKVTGLIGHAVSLNKKSSSVGETECGIGNTCSWKMCGIDPAASYGIYFEIANQGGPAPMQQGPHKAMMQFLTYYQHSSGQYHLRVTTVARPI"
  },
  {
    "reference": "MSADRRKVDVILGSQWGDEGKGKLCDILSAQVDVCARSAGGNNAGHTIVADMPVGQGVDGKGGKTTFDFHLLPSGLVNPNCIGLLGSGVVMHVPSFFEELAALESKGLKCADRVLISDRAHLVFDFHQIVDGLKEIELGGSSIGTTKKGIGPAYSSKASRSGLRVHHLIGDFEHFSSRFRTLVEGRFKRYGHFEYNIEAELSRYAEYATRLKPHVVDGVQYIHRALSTGQKRILVEGANALMLDLDFGTYPYVTSSSASIGGVLTGLGLPPQSIGNIYGVVKAYTTRVGAGPFPTELTGTLGEHLQEVGAEWGTTTGRRRRCGWLDVVVLNHSRLVNGYTSWNLTKLDVLDDLEELKIGVAYELNGQEMDYFPADLDVLEKVKVRYVTLPGWKQPIKNCKTFEELPAECRAYVETVEQLTGIKVRWIGVGPGRAAMIDRV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be able to bind IMP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "FPRHDADIHKFARGHVVVLGGETMTGAARLVAGAARRGGAGLVSLAAPEKAFPIYAASVDPGTLTPSFKGVKGFSALISDARKTTCVMGPGAGLGAGQGKTTRAITLAAINAGKRCVLDADALSVFQKDPDVLFKALKAEAKMLGGLVATVLTPHGGEFQRLFPDLARRYARGGTRTKHSKIGITLEAAKRSGAVVLFKGPDTVVAAPDGRTAVTTNAPRTLATAGAGDVLAGLIGALLAQGMPAFEAANGAAWLHGEAASAFGPGLIAEDIIDELPAR",
    "response#2": "DINVWGEAFPRHDADIHKFARGHVVVLGGETMTGAARLVAGAARRGGAGLVSLAAPEKAFPIYAASVDPGTLTPSFKGVKGFSALISDARKTTCVMGPGAGLGAGQGKTTRAITLAAINAGKRCVLDADALSVFQKDPDVLFKALKAEAKMLGGLVATVLTPHGGEFQRLFPDLARRYARGGTRTKHSKIGITLEAAKRSGAVVLFKGPDTVVAAPDGRTAVTTNAPRTLATAGAGDVLAGLIGALLAQGMPAFEAANGAAWLHGEAASAFGPGLIAEDIIDELPAVLDWV",
    "response#3": "DINVWGEAFPRHDADIHKFARGHVVVLGGETMTGAARLVAGAARRGGAGLVSLAAPEKAFPIYAASVDPGTLTPSFKGVKGFSALISDARKTTCVMGPGAGLGAGQGKTTRAITLAAINAGKRCVLDADALSVFQKDPDVLFKALKAEAKMLGGLVATVLTPHGGEFQRLFPDLARRYARGGTRTKHSKIGITLEAAKRSGAVVLFKGPDTVVAAPDGRTAVTTNAPRTLATAGAGDVLAGLIGALLAQGMPAFEAANGAAWLHGEAASAFGPGLIAEDIIDELPAVLDWLDR"
  },
  {
    "reference": "MVKHMNNSLEYAELLDEQDNLKHLRSLFHFPQYNSKKSTYFCGHSLGLQPKETESFVSQELKDWKDMGVLGHFDAKNPWYYYHEYLNKQLSELIGGLSEEVVCMNSLTVNLHLLLTSFYRPNSEKYKIVIDTPCFPSDRYAINSHIKLHGLQLEDSLIELAPEPNQLTLNEDLIVEEITKNADQIALVLISPVNYFTGQYYDIERISKVAKDNNCVIGLDLAHTIGNVKVFLHDWEIDFAVWCSYKYLNGGPGAPGGAFIHSKHLKNEDLNRLEGWWGNDSKTRFNMLNEFNPSNTAESWQLSNPPILSMAALLCSLNIFRSVGMNDLVAKSIKMTSYLYSLLEKAENLSIITPRKTEHRGAQLSIKIENFNPNIYTKLIENGFICDLRKPDVLRIAPVPLYNSFEEIYHFSNFLKSLINE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. We are looking for a protein with broad substrate specificity for H2O + L-kynurenine = anthranilate + H(+) + L-alanine.2. For general function, the protein need meet that Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively.3. The designed protein should have a binding site that is specific to the target pyridoxal 5'-phosphate ligand.4. A protein that is capable of catalyzing reactions within the Cofactor biosynthesis, NAD(+) biosynthesis, quinolinate from L-kynurenine: step 2/3 pathway with high efficiency and specificity.5. The presence of Aminotransferase class V domains is critical for the interaction of the designed protein with its intended targets.6. pyridoxal 5'-phosphate is required for the proper folding and stability of the protein.7. A protein that can perform 3-hydroxykynureninase activity in cytoplasm to support 'de novo' NAD biosynthetic process from tryptophan, tryptophan catabolic process, quinolinate biosynthetic process, L-kynurenine catabolic process. The designed protein sequence is ",
    "response#1": "MADDRAAEMIAPIVGAQTSEVAVMQTLTANLHLLLSAFYQPKADSRHKIILEKKAFPSDHFAVETQIRHHGLAVESSMVMIETLRSESTALTTQDVVAVIEEHAKDTAVLLLPGIQYYTGELLDIPTITELAHRHGIFVIWDLAHAVGNVPLRLHDWNVDAAVWCSYKYLNSGPGGIGGLFVHTQNSGVIRPKQFDPLPSLLPVHHRVLSVRAGDPDQGWRWLELEEGNGQWNRAGSQSVPLDWDVGWFLGGLAAHVGADDRAAEMIAPIVGAQTSEVAVMQTLTANLHLLLSAFYQPKADSRHKIILEKKAFPSDHFAVETQIRHHGLAVESSMVMIETLRSESTALTTQDVVAVIEEHAKDTAVLLLPGIQYYTGELLDIPTITELAHRHGIFVIWDLAHAVGNVPLRLHDWNVDAAVWCSYKYLNSGPGGIGGLFVHTQNSGVIRPKEGQVVLADDRAAEMIAPIVGAQTSEVAVMQTLTANLHLLLSAFYQPKADSRHKIILEKKAFPSDHFAVETQIRHHGLAVESSMVMIETLRSESTALTTQDVVAVIEEHAKDTAVLLLPGIQYYTGELLDIPTITELAHRHGIFVIWDLAHAVGNVPLRLHDWNVDAAVWCSYKYLNSGPGGIGGLFVHTQNSGVIRPVR",
    "response#2": "MSTPTAASTAAAATAQATAGPASGPPYGPPYGPPPGPPPGPPPGPPPGPPPGPPPGPPPGPPPGPPPPTAPAGGVPYEQLLDHVQHGRVVVDAHGRLAAVNAQAAALLDLPYALLREGIGALTPALQDLFLASGTDTTTSRLVLGGEGRWLLMHSRPWPGGGAVLTLDDRTALRAQAEAARAEARAAQGADEAARAASQEAARVAAEALAAQEEERSRVLADLGAAVGEMEARLDTVSRMAAELAEGTAESAGSAAAVGAAAGDLAELAGRIEAMVRGFQLDSRA",
    "response#3": "MADDRAAEMIAPIVGAQTSEVAVMQTLTANLHLLLSAFYQPKADSRHKIILEKKAFPSDHFAVETQIRHHGLAVESSMVMIETLRSESTALTTQDVVAVIEEHAKDTAVLLLPGIQYYTGELLDIPTITELAHRHGIFVIWDLAHAVGNVPLRLHDWNVDAAVWCSYKYLNSGPGGIGGLFVHTQNSGVIRPRLADDRAAEMIAPIVGAQTSEVAVMQTLTANLHLLLSAFYQPKADSRHKIILEKKAFPSDHFAVETQIRHHGLAVESSMVMIETLRSESTALTTQDVVAVIEEHAKDTAVLLLPGIQYYTGELLDIPTITELAHRHGIFVIWDLAHAVGNVPLRLHDWNVDAAVWCSYKYLNSGPGGIGGLFVHTQNSGVIRPGMYASVLGDLARIIGAKPNEVALMNGLTVNLHLLLVSFYRPSESRYKILMEAKAFPSDRYAIKSQVRHHGYDPKETVIQLAPRKGEHCLRTEDILKTIEAEGDSIAVVLFSGVQFYTGQKFEMQRITDAGHSKGCIVGFDLAHAVGNVKLNVHEWGVDFACWCSYKYMNSGAGGLAGAFIHEKHNSWS"
  },
  {
    "reference": "MDPVDPKLEPWNHPGSQPATACNTCYCKGCCLHCPVCFLNKGLGISYGRKKRRRRHRTPYSNKDHQNPISKQPIPQTQGVSTGPEESKKKVENKTEPDRFA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should contain at least one Nuclear localization signal, RNA-binding (TAR), and protein transduction motif to achieve its intended function.2. Target a Basic and acidic residues, Polar residues compositional bias in the protein's composition for improved properties.3. The Zn(2+) binding site should be stable and able to withstand changes in environmental conditions.4. For general function, the protein need meet that Through its interaction with host TBP, Tat may also modulate transcription initiation; Besides its effect on RNA Pol II processivity, Tat induces chromatin remodeling of proviral genes by recruiting the histone acetyltransferases (HATs) CREBBP, EP300 and PCAF to the chromatin; Other factors such as HTATSF1/Tat-SF1, SUPT5H/SPT5, and HTATIP2 are also important for Tat's function; To ensure maximal activation of the LTR, Tat mediates nuclear translocation of NF-kappa-B by interacting with host RELA; Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to promote processive transcription elongation by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts; Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication The designed protein sequence is ",
    "response#1": "MDPVDPNLEPWNHPGSQPTTGCNNCYCKKCCWHCQLCFLKKGLGISYGRKKRKHRRGTPQSSKNHQNPVPKQPLPTTRGNPTGPEESKKKVASKAETGP",
    "response#2": "MDPVDPNLEPWNHPGSQPKTACNNCYCKRCSYHCQRCFLTKGLGISYGRKKRRQRRRTPPSSEDHQNLISKQPLPQTQGDQKXXKESKDKVEKKTKPDP",
    "response#3": "MEPVDPRLEPWKHPGSQPRTECNNCYCKKCCFHCQVCFICKALGISYGRKKRRQRRRAPQDSQTHQASLSKQPTSQPRGDPTGPKESKKKVERETEKDQRD"
  },
  {
    "reference": "MTTHATTLVFATQNPHKAHEIAQILGSDYHILTLKDIGCAEDIPETEPTLEGNALLKARYVKEHYGYDCFAEDTGLEVEALGGAPGVHTARYAGAAKDTEANLQLLLANLAGHRSRRARFRTVIACLFRGREVLIEGICQGRIATEKRGTGGFGYDPVFIPEGYDQTFAELGETIKNRISHRAIATEKFRLWLRST",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a high affinity for Mg(2+).2. The protein must exhibit nucleotide binding, ITP diphosphatase activity, dITP diphosphatase activity, ribonucleoside triphosphate phosphatase activity.3. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.4. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "MACSTNQSTYL",
    "response#2": "MICLLTGNKNKLAEFR",
    "response#3": "MTSLLIATRNAKKLAELRRVLDAAGLTGLTLVGLDDVPPFPEAPETGATFAENALIKARNAYRLTGLPCVADDSGLCVDALNGLPGVLSARWSGRHGDDAANLALLLAQLSDVPDERRTARFVCAAALVTPDGEEHVVEGKLLGRLVPAPRGEGGFGYDPVFVPEGTGCTSAELLPEEKDAISHRGQAFRALAPRLRTLLA"
  },
  {
    "reference": "MRLKILIKLSGAGMSADSSEPFSNQFLETVIAQLKQLVPNYQIGIVIGGGNIMRGKSCSDYNITEIAGHHLGIMATVINGAFLKAKFDSHKLNSTLLSAVSCPSLATHIVSQTTIDDAFKNHDIVIFAGGTGNPYFSTDTAVALRATQMQADIILIGKNGVDGVYTADPKKDKHAKFLASLTYAEAIKNDLQIMDITAFTMCKENNLKVIIFNINAEHAIIKALSKQGKYTLIEK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must be able to UMP kinase activity.2. The UMP binding site should be located in a region of the protein that is accessible to the ligand.3. We require a protein that can specifically bind to the metabolites produced in the CTP biosynthesis via de novo pathway, Pyrimidine metabolism, UDP from UMP (UMPK route): step 1/1 pathway. The designed protein sequence is ",
    "response#1": "MRIVFDIGGSVLVPENPDIDFIKEIAYQLTKVSEDHEVAVVVGGGKLARKYIEVAEKFNSSETFKDFIGIQITRANAMLLIAALREKAYPVVVEDFWEAWKAVQLKKIPVMGGTHPGHTTDAVAALLAEFLKADLLVVITNVDGVYTADPKKDPTAKKIKKMKPEELLEIVGKGIEKAGSSSVIDPLAAKIIARSGIKTIVE",
    "response#2": "MRIVFDIGGSVLVPENPDIDFIKEIAYQLTKVSEDHEVAVVVGGGKLARKYIEVAEKFNSSETFKDFIGIQITRANAMLLIAALREKAYPVVVEDFWEAWKAVQLKKIPVMGGTHPGHTTDAVAALLAEFLKADLLVVITNVDGVYTADPKKDPTAKKIKKMKPEELLEIVGKGIEKAGSSSVIDPLAAKIIARSGIKTIVKK",
    "response#3": "MRIVFDIGGSVLVPENPDIDFIKEIAYQLTKVSEDHEVAVVVGGGKLARKYIEVAEKFNSSETFKDFIGIQITRANAMLLIAALREKAYPVVVEDFWEAWKAVQLKKIPVMGGTHPGHTTDAVAALLAEFLKADLLVVITNVDGVYTADPKKDPTAKKIKKMKPEELLEIVGKGIEKAGSSSVIDPLAAKIIARSGIKTIVP"
  },
  {
    "reference": "MAEAITIARPYAVAVYRLAKEKNALADWSQMLALAAAIAADEQMRAFIDNPKVDAADLERVFLSVSGDRLNEAGTNLIKLLVEYGRLSILPEIAAAFEALKAQDEGVLEAEITAAVQPSDAEFSAIVKRLEAKFGKKVEASVKVDPEIIGGIKIVVGDTVIDASVRGQLQELAYTLKG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that This protein is part of the stalk that links CF(0) to CF(1); It either transmits conformational changes from CF(0) to CF(1) or is implicated in proton conduction.2. The designed protein localizes to the plasma membrane. The designed protein sequence is ",
    "response#1": "MTISPL",
    "response#2": "LIPL",
    "response#3": "LVSL"
  },
  {
    "reference": "MSHRQSCDRCRQQKVRCLRDESQGGAGNKARASFSRCERCTKASVDCVYSLKSRSNRPNAHQPIHNQVDADPQTLVGPSWFGQGLFCQAASGSLGPDYDDLAGQFADLDGAEFNGWDGNLDVIPSLSMSSLNTLAAPAAMVSQDISPTKDHHDNNHMQEPEADDDDESNNGHDQDAIQELSLQLTAISQRATRAKRRLHRAGCPPPTVSSPEVNEAFEDTNNLLRIANNITNACAHLCDEASSNSITLDYGLVFSALASHQHLLALFKAICHLIQRYLESMTSANQHRQQQQQRLHDGDIGPSSVAQFVMVLQLLTYLINRMDRNLAQVNGPHGHGLETSLGGQATPITPSTGYQDIVQSMLIHRGDKNNTTGSSQSLPIIGQVVLGAIPDEHKRLRQVIQELQTRIERSEIQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must be able to DNA-binding transcription factor activity, RNA polymerase II-specific, zinc ion binding, DNA binding.2. For general function, the protein need meet that Transcription factor that regulates the expression of the gene cluster that mediates the biosynthesis of fujikurins. The designed protein sequence is ",
    "response#1": "LQAYVEYVHPYMPLMDLNSFLGIINTRDGQNGQTSLFLYQAVMFAASAFVDMKYLREGGYTTRKAARKSFFQKTRLLYDFDYESDRLVLVQALLLMTYWYETPDDQKDTWHWMGVAISLAHTIGLHRNPGSTSMAPAKQKLWKRIWWSCFMRDRLIALGMRRPTRIKDEDFDVPMLEESDFEIEVLADDNTVIPASCTLVRNLDMQRELAIMCISKAQLCVCISHMLKAQYSVLIRDKMKPENTTNSTMMLFPNKQLDNVEGVTEVDHELMAWR",
    "response#2": "LQAYVEYVHPYMPLMDLNSFLGIINTRDGQNGQTSLFLYQAVMFAASAFVDMKYLREGGYTTRKAARKSFFQKTRLLYDFDYESDRLVLVQALLLMTYWYETPDDQKDTWHWMGVAISLAHTIGLHRNPGSTSMAPAKQKLWKRIWWSCFMRDRLIALGMRRPTRIKDEDFDVPMLEESDFEIEVLADDNTVIPASCTLVRNLDMQRELAIMCISKAQLCVCISHMLKAQYSVLIRDKMKPENTTNSTMMLFPNKQLDNVEGVTEVDHELMAWL",
    "response#3": "LQAYVEYVHPYMPLMDLNSFLGIINTRDGQNGQTSLFLYQAVMFAASAFVDMKYLREGGYTTRKAARKSFFQKTRLLYDFDYESDRLVLVQALLLMTYWYETPDDQKDTWHWMGVAISLAHTIGLHRNPGSTSMAPAKQKLWKRIWWSCFMRDRLIALGMRRPTRIKDEDFDVPMLEESDFEIEVLADDNTVIPASCTLVRNLDMQRELAIMCISKAQLCVCISHMLKAQYSVLIRDKMKPENTTNSTMMLFPNKQLDNVEGVTEVDHELMAWQR"
  },
  {
    "reference": "MQATNIVILAAGNGSRMKSDLPKVLHQVAGQAMVDWVIDAVQPLKPNKILTVTGVGAERVQDHLAGKTDFVIQEKQLGTGHAVLQTKAALADSQGATLILSGDTPLFTTETLQALLNEHSRTKNAVTVLTAKADNPTGYGRIVRGDDGMVLKIVEEKDASVTERRIEEINTGVYIFDNQLLFQALSQVGNDNVQQEYYLPDTLDILRGMGKQIGAYQTLDFSESMGVNDRVALAQANQIMRNRINQMHMKNGVELVAPDQTFIDAGVVIGKDTLIEGNVTLKGQTVIGRDNVLTAGTRIEDSTIGDGNVITSSHLEEAVVKDRTTIGPYAHLRPAAEIMDEAHLGNFVEVKKATIGQGSKVGHLSYVGNAKLGADVNVGAGTIFVNYDGVNKFDTTVGDRVFIGSNTKIIAPVTLADESITAAGSTITDDVATHAMGIARTRQTNKADFWDRMPHKKG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain..2. The protein design must be able to perform glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity in order to contribute to peptidoglycan biosynthetic process.3. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.4. The designed protein should have a binding site that is specific to the target Mg(2+) ligand.5. Mg(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "MKAIVLAAGGSTRMNTKLAKVLHEVCGRPMLAYVLQACRKVGIKRMYVVVGFSADQVKSRFADADDVVWVEQKEQLGTAHAVMCCREYLQDFEGQTLILCGDGPLLRSQTLRTLIEKHEAGQSAATLLTAEMADPTGYGRVIRDAEGNVQAIVEHKDASPEQLAINECNSGLMAVKSADLKRWLSQLSNDNAQGEYYLTDIVALAVQDGVTIVTAQPEYEFEVEGVNNRLQLAALERAFQARQAEQLMVDGVTVVDPSSVWVDVTVTFERDAVVHPGTQLLGATHLGEDAEVGPNSRLRDTVVGRGARVDNTVADGAEVGEAASVGPFAYLRPGTRLGAKAKAGTYVEMKNASIGEGTKVPHLSYVGDATIGEYTNIGAASVFVNYDGEAKHHTTIGSHCKTGSDNMFVAPVTVGDGAYTAAGSVITKDVPAGSLAVARGQQRNISGWKRPVKK",
    "response#2": "MGIILAAGEGKRMKSNLPKVLHKVCGKAMVEHVINALHSVVDDYTIVVGHGADKVKGYLGDKFKFSYQDKQLGTGHAVMCAEEFLKGKRGTVIILAGDTPLVTSKTISEVFQYHIKKGYSATVITGVVENPKGYGRIVRDSEGNLERIVEDKDANELEKQIKEVNSGAYCFDIEDLLNALKRLNNNNAQGEYYLTDVIEILKNDGKKVGAYVTDDPSEAIGINTRVQLSQAEQVMRRRINRKHMLNGVTITDPETTWIDVTVTLARDVVIDPNTQLKGTTTVAEGATVGPDVTLIDTTVGARATVVRSHVIGAEVGPEASVGPYAYLRPGTRLARQVKVGTFVETKNADIGVGTKVPHLSYVGDATIGEHTNIGAATVFVNYDGVNKHRTVIGSHARTGADNMFVAPVTIGDGAYTAAGSVIVKDVPPGALGVARGQQRNIEGWVTKKK",
    "response#3": "MTAVVLAAGKGTRMKSKQYKVLHHVAGKPMIRHVIDQVRESGVDNVVTIVGHGAESVKETLGDTSLYSFQEEQLGTAHAVKQAAEHLASKEGTTLVVCGDTPLITSATLSQLLAHHDQEGAQATVLSAIAAQPFGYGRIVRDETGQLTEIVEEKDADAQQKKIQEISSGIFAFNNQTLFQLLEQVDNDNAQGEYYLPQVLSLVLQNKKAKNVYAYRTNDFEETLGVNDRVALSKAEIIMRKRINEQHMKNGVTIIDPANTYIGTDVTIGSDVVIEPGCVLKGNTTIEGEAVIGPNSEVNDCHIGANTVLRHSVATSSYIGQRVQIGPFAHIRPDASIGNDVKVGNFVEIKKSKIDNKSKVPHLSYIGDAEIGSGVNIGCGAITVNYDGKNKFKTTIEDDAFIGCNANLIAPVTIGKGALVAAGSTINQDVPGDALSIGRAKQVNIEGWVQKKKS"
  },
  {
    "reference": "MKFTTLLFLAAVAGALVYAEDASSDSTGADPAQEAGTSKPNEEISGPAEPASPPETTTTAQETSAAAVQGTAKVTSSRQELNPLKSIVEKSILLTEQALAKAGKGMHGGVPGGKQFIENGSEFAQKLLKKFSLLKPWA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must contain a signal peptide for proper functionality.2. The protein should be optimized for stability and solubility when expressed in secretory granule, extracellular space, extracellular region where it will be involved in defense response to bacterium. The designed protein sequence is ",
    "response#1": "VEFYKRVAVGILGENLVLSPYSVYKAFAMAYAGASGATREELKAVFGFGEDPCVLPAASRGVEEALSAWLQTDFPFKPNYLNKLRCIGAEAKYVDFERDYKSAIAAINKWAEEKTRGLIKDLVPADYPEGWDVRAVLVSALYFKGNWWPDRFQRVGKREFKGAGPADFIALDLSSCGDPSLRGRASSDLTVVELPFNNTEVALYIIMPRDLPSFVKELTYEKLREIISTLPDQVIRVEMPLFKAEFKGSVKQALREMGVVRAFETADFTEMAYRRLYIDDVFHGAYLNADENGVVAAAATAVVFKPVCAKGGGVEVVVDKPFLFVLADRTSGVIYFIGHVVNP",
    "response#2": "GLWKTLKDSAKSAVTNVAVTMLDKLRCKLTGGC",
    "response#3": "GRFAVEFYKRVAVGILGENLVLSPYSVYKAFAMAYAGASGATREELKAVFGFGEDPCVLPAASRGVEEALSAWLQTDFPFKPNYLNKLRCIGAEAKYVDFERDYKSAIAAINKWAEEKTRGLIKDLVPADYPEGWDVRAVLVSALYFKGNWWPDRFQRVGKREFKGAGPADFIALDLSSCGDPSLRGRASSDLTVVELPFNNTEVALYIIMPRDLPSFVKELTYEKLREIISTLPDQVIRVEMPLFKAEFKGSVKQALREMGVVRAFETADFTEMAYRRLYIDDVFHGAYLNADENGVVAAAATAVVFKPVCAKGGGVEVVVDKPFLFVLADRTSGVIYFIGHVVNP"
  },
  {
    "reference": "MYQLGDVKKIVLPGDPVEVQGKIRNGIYRGPDNKYYSEYFGTLQVSDQYVDVVPFSGQYIPRKGDKVIGKIIEVGPSTWTVDINSPYFAMLHMNDTPWRISSGDLKRYLNSGDYVYAKIMSVNEIKESWLTLKEPGLKKLEGGHMVLIHASRVPRVIGKGGGMVNMVKELTSTRIIIGQNGLIWIDGPIEGVTMAIAAIEMIEREAHTEGLTVRVESFLKELRGDKDGNQQDKANQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein can be expressed in cytoplasm and perform poly(A) binding without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "MDSRIGVVGPNGAGKSTMLKLLIEQLHPTSGIVSRHPRLRIAYFAQHHVDTLDLNLNALSFLAKTFPGKGEEEYRRHLGAFGVSGPLALQKMITLSGGQKSRVAFACLGLQNPHILILDEPTNHLDMESMDALTRAVKRFQGGVILVSHDVDFLDKTCTSIWQCDHNVVSKFR",
    "response#2": "MDSRIGVVGPNGAGKSTMLKLLIEQLHPTSGIVSRHPRLRIAYFAQHHVDTLDLNLNALSFLAKTFPGKGEEEYRRHLGAFGVSGPLALQKMITLSGGQKSRVAFACLGLQNPHILILDEPTNHLDMESMDALTRAVKRFQGGVILVSHDVDFLDKTCTSIWQCDHNVVSKFR",
    "response#3": "MDSRIGVVGPNGAGKSTMLKLLIEQLHPTSGIVSRHPRLRIAYFAQHHVDTLDLNLNALSFLAKTFPGKGEEEYRRHLGAFGVSGPLALQKMITLSGGQKSRVAFACLGLQNPHILILDEPTNHLDMESMDALTRAVKRFQGGVILVSHDVDFLDKTCTSIWQCDHNVVSKFR"
  },
  {
    "reference": "MSLTAVILAAGQGTRMKSRLPKVLHPVLGEAMVRWVVRAAQAAGAQRCVVVVGVGAEAVQAELADLAHVDFATQVEQRGTGDALKAALPVLDPSQDERLLVLCGDVPLIDPEELKQLAGSLAERPAAVLTFEAPEPGGYGRMLRNAEGLVSGIVEAKDASPEQLAITEVNSGTYAFETRFVLNNLDRLTTQNAQGEFYLTDLIAFSEGAVGLCTPHPERLEGANDRAGLAALNASARDWVNGQHMANGVGMEDPHRIWIGPRVCIGSDSFLESDTRLMGETVLGEQVHVHQGCQLRDSTVGDGSVLHPYSVLEEAKVGAGCSVGPYGRLRPAAEXXDGAKIGXFVEVKKAVLGPGAKANHXAYIGDASVGAGANVGAGTITCNYXGANKHKTEIGEGSFXGSXSTXVAPVXIQDQAYVAAGSVITMEVPEDALGVGRTRQRNIKGWVSRKAPKKDK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc)2. The protein must bind to Mg(2+) in order to perform its enzymatic function.3. The UDP-N-acetyl-alpha-D-glucosamine binding site should be stable and able to withstand changes in environmental conditions.4. The protein should have a fast turnover rate for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.5. A protein that is capable of catalyzing reactions within the UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "VIILAAGEGTRMRSATPKVLHTLAGRSMLGHALVAARSLDPGRLAVVVRHGRDAVAAHALELDPEVLLADQDDVPGTGRAVQIGLWVLDAATQAAVAGESSGDGPGVSGVATQVRLEGAVVVLAGDIPLLDGGTLAELLAAHTADGNAVTVLTTVLDDPTGYGRIVRDAAGAVAAIVEHKDASEAQRAVREINSGVFAFDGKLLADALGKVRTDNSQGEEYLTDVLGILRAAGHRVGASVAGDHREILGINNRVQLAEARRLLNERLLERAMLAGVTVIDPASTWIDVDVMIGRDTVVRPGTQLLGGTRIGSRCDIGPDTTLTDVTVGDAAEVVRTHATSAEIGDEATVGPFTYLRPGSKLGSAGKLGAFVETKNAAIGAGSKVPHLTYVGDADIGEHSNIGASSVFVNYDGETKSRTTIGSHVRTGSDTMFVAPVTVGDGAYTGAGTVIRDDVPPGALAVSAGPQRNIEGWVVRHRAGTAAAEAAQRALAAQEAGSDSDPESAGAGADAQEQE",
    "response#2": "VIVLAAGEGTRMKSAKAKVLHSVAGRSLLGHVLHAVDHLKAKQVRIVVGSGRELVEEHISHIAPKASTVFQERRGGTGHAAQLALAGLSAKGTVLVLAGDTPMLTGESLAAFIDAHNEGKFAASVLTAEHPDPTGYGRIIRDDAGELGRVLRAGDGSVLAVREEKDASEAERAIGEINSGVYAFDAAVLREALARVTTDNAQGEMYLTDVLAIARDAGGSVAAALAPDAWTEVGAGAFIGSNTALVAPVTIGPGAFIGSGSVITDGVTVGDDARIGPFARLRPGATLANDVHIGNFVEVKAGAQHEQIVVGPRAAIDPGAELTGPCRILGATTIGQGATIGAFCQITNCVIDAGATVREFCHLEDAHVCAGAVAGPYSRLRPGADVGENARVGNFVEMKKASLGKGAKASHLTYLGDTAVGAGANIGAGTITCNYDGVHKHQTNIGAGAFIGSNTALVAPVQVGENALVAAGSVITKTVPDGALAVARARQRNIEGWVARKRAGTKSAEAAERALAERETVEGER",
    "response#3": "IILAAGQGTRMRSDLPKVLHPVAGKPMLQRVLETCKKLTNDRLAVVYGHGGEQVKQLTQEDGIVWVLQAEQRGTGHAVAQAIELAPDEAIVLIAYADTPLIRSETLQQLIAHLGDAALAVLTSKPVSPTGRLGRLVMVDGKLQAIREHKDASEAERAVDLCNSGVMAVDGRLLFDLLGKIGNDNAKGEYYLTDIVELARASGHKADAVEAPEAEVLGANDQHEQIVVGPRAAIDPGAELTGPCRILGATTIGQGATIGAFCQITNCVIDAGATVREFCHLEDAHVCAGAVAGPYSRLRPGADVGENARVGNFVEMKKASLGKGAKASHLTYLGDTAVGAGANIGAGTITCNYDGVHKHQTNIGAGAFIGSNTALVAPVQVGENALVAAGSVITKTVPDGALAVARARQRNIVDGWAARFRAAQTAKKKS"
  },
  {
    "reference": "MGEILDGKKLARELGEKLGSEVDSLKEHGVSPKLCVINIGDDPASKVYVASKKRKAEKLGIKQVVYQLPADESEEDVLKLIDSLNADPEVSSLMVQLPVPPQINADRVIERIDPEKDVDCLTPANIGRLWQGKHFVEPATAAGIIALLDHYQIDLTGKNAVIVGRSNIVGKPLAALMLERNASVSILHSRSRNLADLTKQADILVCAVGKAEMIKADMVKEGAVVIDVGINRVDGHLVGDVDFAPVKEKASWITPVPGGVGPLTVEFLMEEVIKLTRRQHGLD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The NADP(+) binding site should be located in a region of the protein that is accessible to the ligand.2. The protein should be designed to have high stability and activity towards 5,10-methenyltetrahydrofolate + H2O = (6S)-10-   formyltetrahydrofolate + H(+).3. The protein must exhibit the following characteristics: Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10- methenyltetrahydrofolate to 10-formyltetrahydrofolate..4. The protein should be optimized for activity in One-carbon metabolism, tetrahydrofolate interconversion pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. The protein design must be able to perform methylenetetrahydrofolate dehydrogenase (NADP+) activity, methenyltetrahydrofolate cyclohydrolase activity in order to contribute to methionine biosynthetic process, tetrahydrofolate interconversion, purine nucleotide biosynthetic process. The designed protein sequence is ",
    "response#1": "MSGKPLHEKTKEWARRHVRLLEEVGVTPKLAVLLLNDDPVELETQRRYVSLKARDVREVGGEVEIYELVDVPPERRSKEALYLIERLNRRDDVTGILIQKPVPPFVDEDLLFQRLSPEKDVDGLTPGGVGPMTEVNAGRLSLGIDGIVPCTPLGCLMLLQSVHKDMTGMHAVVIGASNLVGKPMAQLLLKENCTVTVAHIHTRNTKELAREADILVVATGKSELVRGDWIKPGATVIDVGITRVPAENGKTRLVGDVAFDEAFPIAGHITPVPGGVGPMTIACLLHNTLQAARPAKDVDGFHPENVGKLVGGTPRFKPCTPHGVQKLLAHQGVDPDGAEVVVVGRSNIVGKPLANLLAQKAAGGNATVTICHSHTDDLAAHTRRADVVVAACGVPELIDGDMLSGDPVVIDVGINRVDADTEKGYELVGDVDYDSAEAAASAITPVPGGVGPMTRAMLLYNTLAAPYNQGLIALNREFLVPATPRAVIDIMDYYGYHENTVTIVNRSPVVGRPLSMMLLNRNYTVSVCHSKTKDIGSMTRSSKIVVVAVGRPGFLNREMVTPGSVVIDVGINYVNDKVVGDANFEDLSEYVEAITPVPGGVGPITATNILENVVKAAEFL",
    "response#2": "MSGKPLHEKTKEWARRHVRLLEEVGVTPKLAVLLLNDDPVELETQRRYVSLKARDVREVGGEVEIYELVDVPPERRSKEALYLIERLNRRDDVTGILIQKPVPPFVDEDLLFQRLSPEKDVEGLNFAAPYNQGLIALNREFLVPATPRAVIDIMDYYGYHENTVTIVNRSPVVGRPLSMMLLNRNYTVSVCHSKTKDIGSMTRSSKIVVVAVGRPGFLNREMVTPGSVVIDVGINYVNDKVVGDANFEDLSEYVEAITPVPGGVGPITATNILENVVKAAEFLF",
    "response#3": "MSGKPLHEKTKEWARRHVRLLEEVGVTPKLAVLLLNDDPVELETQRRYVSLKARDVREVGGEVEIYELVDVPPERRSKEALYLIERLNRRDDVTGILIQKPVPPFVDEDLLFQRLSPEKDVEGMEVNAGRLSLGIDGIVPCTPLGCLMLLQSVHKDMTGMHAVVIGASNLVGKPMAQLLLKENCTVTVAHIHTRNTKELAREADILVVATGKSELVRGDWIKPGATVIDVGITRVPAENGKTRLVGDVAFDEAFPIAGHITPVPGGVGPMTIACLLHNTLQAARPDKDADGLHPVNLGRLVRGEKGLRSCTPAGVMRLLAEYEISLRGKQAVVVGRSILVGKPMALMLLEADATVTIAHSRSQDLKSITQNADILVAAAGLPGLITADMVKPGAVVVDVGINRVTDANGKSRLVGDINFASIAGVAEYITPVPGGIGPMTVALLLQNTVTSVTRYTSPEKDVDALTPENKKRLLALFDLDRDLLPCTPAGILELLQMYGVEVKGRDVTVVGKGELVGKPLAVMLMQLDASVSVLHALTRDKLRYVKEADIVISAVGRPPELYRDNPWRLTGDMVKEGAVVVGVGGKVDPATGKWYFDVDEKSVAEKASYLTPNLGGVGLATRARLLKNLIRAEITQLTN"
  },
  {
    "reference": "MAKRKYIFVVGGVMSSVGKGITSASLGALLQNTGLCVTAVKADPYINVDAGTMNPTEHGEVFVTRDGDETDQDMGNYERFLDTELTATNYMTTGRVYQTVIERERSMAYGGKCVEVVPHIPEEIIRRIKDAARVNKADVTIIEIGGTVGEYQNLLFLEAAREMKLRNPKDVVFVLVSYLPIPANVGEMKTKPTQYASRTLNGTGIQADFIVCRGERPIDEPRRRKLATSCGVAPEAIISAPDMPSIYEVPLLLEDAGMGTMVLKRLGMPSRSRTMKEWRVFIKKIQHAQRTVKIAVVGKYFATGDFTLKDSYISVIESLKHSAWFQNCKPELTWIDATVYEKQPSRVKELKGYDGVLVPGGFGSRGIEGIVAAIRFVRERKIPFFGLCYGMQLACVEIARHVAGLQGAHSVEVKPDTKHPIIIANPYQQKNLVNKRYGGTMRLGAYPCVIAPGTRARAAYGSARIEERHRHRYEFNNEYRERLEKAGLVFSGVYPEQNLVEIIELPSHPWFVAVQFHPEFKSRPLRPHPLFRAFIAAAIKRG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in 'de novo' CTP biosynthetic process, glutamine metabolic process, potentially with improved properties such as stability, specificity or selectivity.2. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The protein should be able to bind L-glutamine ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "LTIGLIGKYVELQDSYKSILESFIHAGAANNVKVNVESIHSEYLKSDNAKLKLAHLDGVLVAPGFGERGIEGKIDAVKYVRENNIPFLGICLGMQMAVIEFSRNVLGIEDACSTEMDATTKNPVIDLMEDQKTITDKGGTMRLGAWTCDLKEGSKVRDIYNAESIKERHRHRYEFNSNYREQMEAAGMKATGLNPETGLVEIVEIPSHPWFVGVQYHPEYKSTVANPHPLFVAFVEAAYKK",
    "response#2": "LQDAYKSILEALIHAGAINECKVEVRNVHSEHLTVENAVEQLQNLDGILVAPGFGQRGIEGKIEAIRYARENGIPFFGICLGMQMAVVEFARNVLGHTDAFSTEMDPETPHPVIHLMEEQKNIEHMGGTMRLGSYDCEITPGSILAGAYGTKTVQERHRHRYEFNNAYLDEFEANGMRAVGKNPQTGLVEAVEVPTHPFFLGAQFHPEYKSTVEAPAPLFVSFIAS",
    "response#3": "LQDSYKSILESFIHAGAANNVKVNVESIHSEYLKSDNAKLKLAHLDGVLVAPGFGERGIEGKIDAVKYVRENNIPFLGICLGMQMAVIEFSRNVLGIEDACSTEMDATTKNPVIDLMEDQKTITDKGGTMRLGAWTCDLKEGSKVRDIYNAESIKERHRHRYEFNSNYREQMEAAGMKATGLNPETGLVEIVEIPSHPWFVGVQYHPEYKSTVANPHPLFVAFVSH"
  },
  {
    "reference": "MSESLGSVPGSLRSGVISPGLSLGADISSFTEYLRAHAPDQLPQARAEAMGLHRAADDIPHGTTIVAVSYPGGVILAGDRRATMGNLIATRDVKKVYITDEYSAAGIAGTAGIAIEMVRLFAVELEHYEKLEGVPLTLDGKVSRLASMVRGNLGAALQGLAAVPLLVGYDIDHDDPTERGRIFSFDVAGDRHEEFGGYQAIGSGSVFAKSSLKKLYRHDLDEASALAIAVEALYDAADDDSATGGPDIVRKIFPLAAVVDAGGAREVDAESIEAAARAIVERRAAEHEGGPR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.2. A protein that contributes to modification-dependent protein catabolic process, proteasomal protein catabolic process by functioning in proteasome core complex, beta-subunit complex, cytoplasm with threonine-type endopeptidase activity.3. A protein that is capable of catalyzing reactions within the proteasomal Pup-dependent pathway, Protein degradation pathway with high efficiency and specificity.4. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile to enable effective catalysis of Cleavage of peptide bonds with very broad specificity.. The designed protein sequence is ",
    "response#1": "VLLYGPPGCGKTLIAKAVAASLAGMVDPHGTEGATSYFLNVKGPELLNKYVGETERHIRLIFARAREKASQGMPVVVFFDEMESLFRTRGTGLSSDVETTIVPQLLAEIDGVERLDNVIVIGASNREDMIDPAILRPGRLDVKIKIPFM",
    "response#2": "PPKGVLLYGPPGCGKTLIAKAVAASLAGMVDPHGTEGATSYFLNVKGPELLNKYVGETERHIRLIFARAREKASQGMPVVVFFDEMESLFRTRGTGLSSDVETTIVPQLLAEIDGVERLDNVIVIGASNREDMIDPAILRPGRLDVKIKIERPGLDERVAILRKKAEAEGFDIPDDVYEYIATNIESNIRELEGALIRAVAYASITGLPITIDTAAKNLKDIITGGQREMVTVKEVLDGDRVLVVGRADEERVVRLAGSVRDLPLRVGDSLTVDVRSGFVFEVVR",
    "response#3": "VLLYGPPGCGKTLIAKAVAASLAGMVDPHGTEGATSYFLNVKGPELLNKYVGETERHIRLIFARAREKASQGMPVVVFFDEMESLFRTRGTGLSSDVETTIVPQLLAEIDGVERLDNVIVIGASNREDMIDPAILRPGRLDVKIKIGD"
  },
  {
    "reference": "MSEHERTFVMVKPDGVQRGLIGDIVSRFEDRGLKMVGGKFMQIDQELAEEHYGEHEDKPFFDGLVDFITSGPVFAMVWEGQDATRQVRTMMGETDPAESAPGTIRGDYGLDLGRNVIHGSDHEDEGANEREIELFFDEAELVDWDQIDSSWLYE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.2. The designed protein should have metal ion binding, nucleoside diphosphate kinase activity within cytoplasm to regulate CTP biosynthetic process, GTP biosynthetic process. The designed protein sequence is ",
    "response#1": "LPDAYMSVTEALHHGGIEHDAQVKITWINAEELEEPNADLDEIFVGCKGILVPGGFGDRGVEGKIRAIQYAREHEIPFLGLCLGMQCAVIEFARHVAGLTNAHSTEFVPETPHPVIALMEDQQDVEEKGGTMRLGAYPCLLADGSHSRAEYGAGEISERHRHRFEFNNAYRAQLEEKGMVIAGTSPDDRLVEVVEIADHPWFVASQFHPELKSRPNHPHPLFAGFVY",
    "response#2": "LPDAYMSVTEALHHGGIEHDAQVKITWINAEELEEPNADLDEIFVGCKGILVPGGFGDRGVEGKIRAIQYAREHEIPFLGLCLGMQCAVIEFARHVAGLTNAHSTEFVPETPHPVIALMEDQQDVEEKGGTMRLGAYPCLLADGSHSRAEYGAGEISERHRHRFEFNNAYRAQLEEKGMVIAGTSPDDRLVEVVEIADHPWFVASQFHPELKSRPNHPHPLFAGFVR",
    "response#3": "QRSLVLLKPDAVRRGLTGEIISRIERKAGWQITALELRTLDTETLEQHYGEHKGKPFYEPLVEFMASGPVVAMVVEGERVIEGVRALAGPTDPIAAAPGSIRGDYGVIVRENLIHASDSEESAERELKIFFS"
  },
  {
    "reference": "MNRIILPSPAKLNLFLYINGRRADGYHQLQTLFQFLDVGDEIDISITTSNSTIELVNDIAGVATEDNLIYRAAKLLQKKTACQQGAKIAVAKHLPMGGGVGGGSSNAATVLVGLNYLWQTGLSLAQLAELGLSLGADVPIFVAGKTAFAEGIGEILTPCELPEKWYLVLKPNISIETSSIFKDPKLPRNTPERSLSALLAEDWGNDCEKVVREHYSEVEDLIQELLQYAPFRLTGTGACIFAEFASKAEAEKVFNCRPHNMYGFVAKGQNISPLHKKLNLFS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The protein design should take into account the physiological conditions of the isopentenyl diphosphate biosynthesis via DXP pathway pathway.3. The protein should be able to bind ATP ligand in a variety of conditions4. A protein with 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity, ATP binding is needed to enhance terpenoid biosynthetic process, isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway in a specific context.5. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.. The designed protein sequence is ",
    "response#1": "IRTGNGYDVHSFEEGSFLILCGIKIPFHKKLNGHSDADVAFHALTDALLATQGAGDIGTHFPSSDPQWKNAPSEIFLRHALGILKKAGGRIANVDITIIAENPKIGPYRHAMTENLMTILAITPDRISIKATTNEKIGFIGRGEGIAALATVSVLLVKD",
    "response#2": "IRVGNGFDVHRFGPGDHVVLCGVKVPHGRGLQGHSDADVGLHTITDAIYGALAEGDIGTHFPPSEAEWKGMDSAVFLDHAANLARTRGYAISNVDATLICEFPKIGPHAQAMRGRVAEIIGVEVGRVSVKATTTERLGFAGRGEGIACLATVTVS",
    "response#3": "IRVGNGFDVHRFGPGDHVVLCGVKVPHGRGLQGHSDADVGLHTITDAIYGALAEGDIGTHFPPSEAEWKGMDSAVFLDHAANLARTRGYAISNVDATLICEFPKIGPHAQAMRGRVAEIIGVEVGRVSVKATTTERLGFAGRGEGIACLATVTLVRG"
  },
  {
    "reference": "MHINKLKRYKIIALIAAAGIGSRCSSIISKQYIKLAGKPVLFHTVRKLLANQYIDYVRVVINKSHENFYKEAISSIIDTKLLNPVYGGEIRQNSVKLGLESLQEINPDFVIIHDACRPFVSNALINNLTQFMINGQYAGVVPATKIENTMLIVNNDYIESTISRERLRTIQTPQIFNFKELLSCHQSNKKFSDDSSLMLEYKKCVAIINGEKNNFKLTTKEDIYMANFLLEEPKYRIGIGYDIHKFVKAQDNTKNFIRICGVEIEHNMAIEANSDGDVATHAIVDAILGALGCDDIGKYFPSNSPDWKDYNSSYFLSFAATKAKKKGYRVSNLDITIICEEPKISPCKTKMKQFISKMLEIDDEFVNIKATTAEKLGSIGRRKGIAAHASVLLYKLTSCTNFS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be optimized for activity in isopentenyl diphosphate biosynthesis via DXP pathway pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MDFRIGQGYDVHALVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDAIFGAAAMGDIGRHFSDTDAKFAGADSRVLLRECFARVKAAGFSIANVDSSVVAQAPKLAPHIEGMRANIAADLGLPVERVNVKAKTNEKLGYLGRGEGIEAQAAVLLIV",
    "response#2": "MDFRIGQGYDVHALVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDAIFGAAAMGDIGRHFSDTDAKFAGADSRVLLRECFARVKAAGFSIANVDSSVVAQAPKLAPHIEGMRANIAADLGLPVERVNVKAKTNEKLGYLGRGEGIEAQAAVLLILR",
    "response#3": "LSRTDMKLAIAHAILPNLKSEILKPVDLLSLNLKFEKIDIKKYPIFELKDEVLSNPDLGVIINAANEIAVFAFLQGKCGFLDISSTIFKAVEKFNDLTPENAEDLIKIDKIVRM"
  },
  {
    "reference": "MKVKGTRMNWPNWWKWGTLIIGLGIMCSASDNLWVTVYYGVPVWRDVKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQEMVMGNVTENFNMWKNDMVNQMQEDVISLWDQSLKPCVKLTPLCVTLHCTNVSSSSTDTEMRNCSFNTTTVLKDRKQSVYALFYKLDIVPLNENKTSNERYRLINCNTSAITQACPKVSFDPIPIHYCAPAGYAILKCNNKTFNGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEGDIIIRSENMSDNVKTIIVHLNESVEIKCTRPNNNTRKSIRIGPGQTFYATGDIIGDIRQAHCNISRNNWNKTLQGVGEKLAKYFPNKIIKFTSSSGGDLEITTHSFNCGGEFFYCNTSGLFNSTYNPNGTVSNSSATNESSTITTPCRIKQIINMWQEVGRAMYAPPIAGNITCISNITGLLLVRDGGQENTTNTETFRPGGGDMRDNWRSELYKYKVVEIRPLGIAPTKAKRRVVEREKRAVGQGAVFLGFSGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKSEREIWDNMTWMEWEREISNYTDTIFNLLEESQSQQERNEKDLLALDSWNNLWNWFDITKWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSLQTLTPNPGGPDRPGRIEEEGGEQDKSRSIRLVHGFLALAWDDLRSLCLFSYRRLRDLLSIVT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have a specific function within the host cell plasma membrane. The designed protein sequence is ",
    "response#1": "MGAASMTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGIWGCSGKLICTTNVPWNSTWSNKTKQEIWGNMTWMQWEREIDNYTETIYQLLEQSQNQQEKNEQDLLALDKWDSLWNWFSITNWLWYIKIFIMIIGGLIGLRIIFAVLSIVNRVRQGYSPLSLQTR",
    "response#2": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGLWGCSGKLICTTNVPWNYSWSNKTTNEIWENMTWMQWDREINNYTNTIYRLLEDSQNQQEKNERDLLALDSWNNLWSWFDISRWLWYIKIFIMIVGGLIGLRIIFAVLSVVNRVRQGYSPLSFQTN",
    "response#3": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGLWGCSGKLICTTNVPWNYSWSNKTTNEIWENMTWMQWDREINNYTNTIYRLLEDSQNQQEKNERDLLALDSWNNLWSWFDISRWLWYIKIFIMIVGGLIGLRIIFAVLSVVNRVRQGYSPLSFQTN"
  },
  {
    "reference": "MSSADGALVLFSGGQDSTTCVAWALKRYAKVETIGFDYGQRHAVELEVRPDVLRSLRDINPEWNKKLGEDHMIDLSLISKISNTAMTQDVEITMMENGLPNTFVPGRNLLFMTVAATVAYRRGLDVLVGGMCETDFSGYPDCRDDTMKALQVALNLGMATRLKLETPLMWIDKAETWKMAQDLGGKPLVDLIRAGTHTCYLGERGALHDWGYGCGTCPACELRARGYANFAAQEK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a low Km value for 7-carboxy-7-deazaguanine + ATP + NH4(+) = 7-cyano-7-   deazaguanine + ADP + H(+) + H2O + phosphate.2. We require a protein that can specifically bind to the metabolites produced in the Purine metabolism pathway.3. The protein should be able to bind ATP, Zn(2+) ligand in a variety of conditions4. The protein design must be able to perform ATP binding, zinc ion binding in order to contribute to queuosine biosynthetic process.5. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).. The designed protein sequence is ",
    "response#1": "MNTQTWPVSSEHLNQMRMIGVFDSVADTXDVLAPWYXGXVXPEXXDLXXLXXKQXXQXXQXSXSXSXXIXDXMXXSXSXXTXXXT",
    "response#2": "MNEDVTILGTGSAHPIKVVTNQDIAEIVDTSDEWIRSRTGIRERHISNDESLSSIGLEAARKALADAGLSASDLTQVIVATTTPDAMFPSVACQVQGLLGTTAACAXDXGAQXGMFAIKNASDFIRSGXYHCALCAGGXLSSLVDXSRXTSXLWGXGXGSXGXPXGGXGXXGXSXGXSXXSXGXFHXAVXSXGGAVYGYAXLTLXGXXXVXRAXTXAGXDXKXFXVXGXGXGXPXTXPXXVXXXDXMXATXGDXGXGSXALXSLXYXT",
    "response#3": "MKKIAILGTGNIGGIALEAWKNANFLFEIAAVYDVNRTKVGKDLGKKLGVKYTDNINDLLSLDIDVAIIATPNSLHYEHTKAFLEKGVHVLVEKPMTLNSKEVEELISLAEKKDLVLQVGHIERFNAAYKSVQKYIENPRFIEAHRLAPFNPRGTDVDVILDLMIHDLDIVINLIGSTPKSIDANGVSILTDKVDIANVRLTFEDGCIANITASRVSNKKIRTMSIFQKDSYISADFITQKADIYKLTREEVKDIFFGEIDKTEPLINSVEELEALIVKIINS"
  },
  {
    "reference": "MTDAPQAPITQDVVTFPRKVEDGPRRNLVGLTRAQLQAALIEAGTPEKQARMRTGQVWAWIYQRGVRSFDEMTNLAKPFRALLADRFEIALPEVVRREVSSDGTRKYLLRIAGGHEVETVYIPEENRGTLCISSQVGCTLTCSFCHTGTQRLVRNLTPGEIVGQVMVARDDLGEWPEPGRNPKDETRLLSNVVLMGMGEPLYNFENVRDAMKIVMDGEGISLSRRRITLSTSGVVPEIARCAEEIGCLLAVSFHATTDEVRDRLVPINKRWNIAALLDALRSYPRLSNSERITFEYVMLKGVNDSDEDARRLVELIAGIPAKINLIPFNEWPGAPYERSSNNRIRAFADIIYKAGYASPIRTPRGEDIMAACGQLKSDTERARKSRAQIAAETGA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must possess 4 iron, 4 sulfur cluster binding, metal ion binding to support rRNA base methylation under certain conditions.2. A key design requirement for the protein is the optimization of the Proton acceptor, S-methylcysteine intermediate to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.3. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.4. The protein should be able to bind S-adenosyl-L-methionine ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "SMPPKHFADYTKPERIEILKELGLPKFRDNQIARQYYGRFQADPKKMTDLPESVRERIAETLFPPLMEPVRETSADDGNTQKMLWRLHDGTLLESVLMEYPDRATLCISSQAGCGMACPFCATGQGGLDRNLSTGEIVDQVRAAAATMQAKGGRLSNIVFMGMGEPLANYNRVLSAIHQITQPSPEGFGISQRGITLSTVGLAPAIRRFAKEGLSCRLAVSLHTPDDELRDSLVPANNRWPVEEVLDAAKEYADASGRRVSIEYALIKDINDQPWRADLLGKKLHGALGAKVHVNLIPLNPTPGSKWDASPKRVQDEFQRRVIARGVTCTVRDTRGQEIAAACGQLAAEERSGR",
    "response#2": "SMPPKHFADYTKPERIEILKELGLPKFRDNQIARQYYGRFQADPKKMTDLPESVRERIAETLFPPLMEPVRETSADDGNTQKMLWRLHDGTLLESVLMEYPDRATLCISSQAGCGMACPFCATGQGGLDRNLSTGEIVDQVRAAAATMQAKGGRLSNIVFMGMGEPLANYNRVLSAIHQITQPSPEGFGISQRGITLSTVGLAPAIRRFAKEGLSCRLAVSLHTPDDELRDSLVPANNRWPVEEVLDAAKEYADASGRRVSIEYALIKDINDQPWRADLLGKKLHGALGAKVHVNLIPLNPTPGSKWDASPKRVQDEFQRRVIARGVTCTVRDTRGQEIAAACGQLAAEERSGAR",
    "response#3": "SMPPKHFADYTKPERIEILKELGLPKFRDNQIARQYYGRFQADPKKMTDLPESVRERIAETLFPPLMEPVRETSADDGNTQKMLWRLHDGTLLESVLMEYPDRATLCISSQAGCGMACPFCATGQGGLDRNLSTGEIVDQVRAAAATMQAKGGRLSNIVFMGMGEPLANYNRVLSAIHQITQPSPEGFGISQRGITLSTVGLAPAIRRFAKEGLSCRLAVSLHTPDDELRDSLVPANNRWPVEEVLDAAKEYADASGRRVSIEYALIKDINDQPWRADLLGKKLHGALGAKVHVNLIPLNPTPGSKWDASPKRVQDEFQRRVIARGVTCTVRDTRGQEIAAACGQLAAEERSGARLARAA"
  },
  {
    "reference": "MDYLSNYFEKVKKDKRSKKAIAFFASLDSVNEIYPQVAEDILKELKDQRSYLKLIASENFSSLATQLAMGNLLTDKYSEGYVKHRFYAGCENVDNIEQLAIDEAKKLFNADHVYVQPHSGSDANLVALWSILVHKIQNKEVEKLSKKNAMELTKDEYEKIRQLLVNQKVMGMSLNSGGHLTHGYRLNISSKFLRSYTYDVDPKTHLLDYKRLETQVKEIKPLILIAGYSAYSRKLDFAKLKEIASTVEAVLMVDMAHFAGLVAGKVFTKNFDPIPYADIITSTTHKTLRGPRGGLILCKNEYKETIDRGCPLVLGGPMPNVIAAKAVAFKEANTKDFQNYSSQVVKNAKALANQLMENGVKILTDGTENHLIVMDVYSSFKLTGRQAETALRSSGITVNRNSIPEDQNGPWYTSGIRLGTPALTTLGMKENEMKRVADVIYDVLKNSKPTLNPKTNGPSKAKSDTDPSILTENQNEIRNLLDNFILYPELVL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier2. Target a Polar residues compositional bias in the protein's composition for improved properties.3. A protein with pyridoxal phosphate binding functions is required to enable methylation. The designed protein sequence is ",
    "response#1": "LEASDPEVAAAIQDELDRQREKIELIASENIVSRAVLEAQGSVLTNKYAEGYPGKRYYGGCEYVDVIETLAIERAKKLFGANFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLAAGGHLTHGSPVNMSGKWFKPVSYGVRRDDQLLDMDEVARLAREHKPKLIIAGGTAYSRVWDFARFREIADEVGAWLMVDMAHFSGLVAGGVHPNPVPHAHVVTSTTHKSLRGPRGGLILTNDEALAKKLNSAIFPGLQGGPLMHVIAAKAVAFAEALRPEFKVYAKNVVENARALAAELRAQGLDIVSGGTDNHLMLVDLRPKKVNGKFTEKALDRAFITCNKNAIPFDPEKPFVTSGIRLGTPAGTTRGFGVAEFREIGKLISEALADR",
    "response#2": "LEASDPEVAAAIQDELDRQREKIELIASENIVSRAVLEAQGSVLTNKYAEGYPGKRYYGGCEYVDVIETLAIERAKKLFGANFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLAAGGHLTHGSPVNMSGKWFKPVSYGVRRDDQLLDMDEVARLAREHKPKLIIAGGTAYSRVWDFARFREIADEVGAWLMVDMAHFSGLVAGGVHPNPVPHAHVVTSTTHKSLRGPRGGLILTNDEALAKKLNSAIFPGLQGGPLMHVIAAKAVAFAEALRPEFKVYAKNVVENARALAAELRAQGLDIVSGGTDNHLMLVDLRPKKVNGKFTEKALDRAFITCNKNAIPFDPEKPFVTSGIRLGTPAGTTRGFGVAEFREIGKLISEAL",
    "response#3": "LQDPELAEILMGELARQSTTLQLIAAENFTSKAVLTALGSPLANKYAEGYPGARHHGGCEIVDVAERLAVERAKALFGAEHANVQSHSGSSAVLATYAALLRPGDTVLAMGLPFGGHLTHGSPANFSGRWFDFVGYGVEAESGLIDYEQVRTLARTHRPKAIVCGAISYPRHMDYALFRAIADEVGAYLIADAAHPIGLVAGGAAPSPVPYADVVCATTHKVLRGPRGGMILCGGELAERVDRAVFPFTQGGAQMHTIAAKAVAFGEAATPAFAAYAHQVVANAKVLAEGLAAEGLALTTGGTDTHMLTADTAPLGLDGRSARGRLAAAGMVLDTCALPHGDVRGLRLGTAALTTQGMGEAEMSQVAKALGA"
  },
  {
    "reference": "MPQTIVLATNNRGKVVEFQRLLTDTPYRIKAQAEFDIPDAEETGLSFVENAIIKARHASRLSKLPAIADDSGLEVDLLNGAPGIYSARFAGPAASDADNNHKLLNRLQGLPREQRTARFQCLLVYMRHAEDPTPLICQGSWEGEILTEARGDNGFGYDPLFYIPECGRTSAQLSPEEKSQRSHRAKAMARLLEKLPKP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design must be able to perform nucleotide binding, ribonucleoside triphosphate phosphatase activity in order to contribute to nucleotide metabolic process.2. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.3. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.4. The protein requires Mg(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "MTITTPIE",
    "response#2": "MLKIGT",
    "response#3": "MSDRNT"
  },
  {
    "reference": "MTTQESIKPLVDRILSNPLQFNAAMISNKSNNNDTSAAPENSSYIVIGKQHNNNSNSTAIAATAESKQIKENNLIDRPNGKKTNTVPKSMAEALLLYTSKNDKDAADATGAKKSAELSTELSTEPPSSSSEDDKVGKEEEEEGEIFHEARDYVEPRKASLKERDNADKGDGEDIGEDIGEDIGEDIGEDIGEDIGENLGSPLATIDDSSNENEKEKRKELSTSISSDDEIEDDEDEDDMDYDSSAMEKELPEEEENDSSSKISEGEKKSLYQDLMENSTVEVNRYEPVNNTKENGNRNPKGEEEEEEEEELKHKSRSITPPVTISNLSNFYQFNENINDRGSLNSTRIVKNWGDKFTNLKPRGLLNHGVTCYTNAAVQAMLHIPSIQHYLFDILMGKYDSTISKNSVSYTLAETSKKMWLPVSKNPRKNVSASYINPKHLISRLDDINCMMSEWQQEDSHEYFMSLMSRLQEDSVPKGHKLIESIIYDIFGGLLKQIVTCKSCGSISKTEQPFYDLSLHLKGKKKLDPNSDLSSDSINGTSATTSTTTSNAATKPSLSSSSSVNLNNGSPFAAASDLSSANRRFSIEKSIKDFFNPELIKVDKEQKGYVCEKCHKTTNAVKHSSILRAPETLLVHLKKFRFNGTSSSKMKQAVSYPMFLDLTEYCESKELPVKYQLLSVVVHEGRSLSSGHYIAHCKQPDGSWATYDDEYINIISERDVLKEPNAYYLLYTRLTPKSVPLPLAKSAMATGNVTSKSKQEQAVNEPNNR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in nucleolus, nuclear replication fork that is critical for ubiquitin-dependent protein catabolic process.2. The protein design should emphasize Acidic residues compositional bias.3. The protein must exhibit the following characteristics: Controls the proliferating-cell nuclear antigen PCNA/POL30 deubiquitination which is crucial for keeping TLS polymerases in check as well as for down-regulating the error-free bypass. The designed protein sequence is ",
    "response#1": "MVRTKADCAGSSSGSYRKAVAARAPRKTFGSSSSGSNHDTSPTGKKSECKYAGGNPVCVRPIPTWQKGIGDFFGSPSTSQPEKENRIPSDDEEAGGSGAGKKPRKSRPLPPDPSEC",
    "response#2": "MVRTKADCAGSSSGSYRKAVAARAPRKTFGSSSSGSNHDTSPTGKKSECKYAGGNPVCVRPIPTWQKGIGDFFGSPSTSQPEKENRIPSDDEEAGGSGAGKKPRKSRPLPPDPSEC",
    "response#3": "MVRTKADCAGSSSGSYRKAVAARAPRKTFGSSSSGSNHDTSPTGKKSECKYAGGNPVCVRPIPTWQKGIGDFFGSPSTSQPEKENRIPSDDEEAGGSGAGKKPRKSRPLPPDPSEC"
  },
  {
    "reference": "MSNLKLFAGRANLPLASKISDYLGVGLGAVKINAFPDGELMVKLEEDVRGRDVFIIQPTCEPVNETLIELLVFIDCARRASAERITAVIPYFGYARQDRKDEGRTPITAKLVANLIATAGADRVLTMDLHAAQIQGFFDIPVDNLLAEPVLSRYFVQRGLENLVLVSPDVGNTKRARVYAERLGGELAIINKRRVSGEEVAVGRIIGDVRDKTVLMMDDMISTAGTVCGAAVLCREEGAKQVFVGATHGVLCGPAIERLGAAPIDEIIVTDTIPVGQDKVQQIGKLRVLSVAGLMGEAIHRIHNDESVSSLFAIENGGQA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must bind to Mg(2+) in order to perform its enzymatic function.2. The designed protein have Ribose-phosphate pyrophosphokinase N-terminal domains with specific sequence motifs that are crucial for its activity.3. The designed protein must possess kinase activity to support nucleotide biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "MPLFAHYYRRRGMVGDDYVVVSPKNSGVQRARSLAEYLDTAIAIVDVAEEGNPESGYVIGDVEGRCCIMVDDILNTGEVFSNASKTLKKAGAKEVVACASHGLLSPPAKENLDAADIKDICG",
    "response#2": "MPLFAHYYRRRGMVGDDYVVVSPKNSGVQRARSLAEYLDTAIAIVDVAEEGNPESGYVIGDVEGRCCIMVDDILNTGEVFSNASKTLKKAGAKEVVACASHGLLSPPAKENLDAADIKDICG",
    "response#3": "MPLFAHYYRRRGMVGDDYVVVSPKNSGVQRARSLAEYLDTAIAIVDVAEEGNPESGYVIGDVEGRCCIMVDDILNTGEVFSNASKTLKKAGAKEVVACASHGLLSPPAKENLDAADIKDICG"
  },
  {
    "reference": "MPEFEVTPWEVTGVVDYNKLIEEFGTTPLTDDLLKKTEELTKKELPMYFKRKFFFSHRDYDLVLKDYEAGKGFFLYTGRGPSGPMHIGHIIPFFATKWLQENFGVNLYVQITDDEKFLFKPQLTFEGTKRWAYENILDIIAVGFDPDKTFIFQDSEFTKIYEMAIPIAKKVTYSMAKAVFGFNEQSKIGMIFFPAIQAAPTFFEEKRSLIPAAIDQDPYWRIQRDFAESLGYYKTAALHSKFVPGLMGLGGKMSASKPETAIYLTDDPEEAGKKIWKYALTGGRATAKEQRELGGEPDKCVVFKWLEIFFEPDEKKLLERYIACKNGEILCGQCKRYLIEKVQNFLKEHQEKREKAKKEIEKFKYTGDLAREQWDKAIPEPLRK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have tryptophan-tRNA ligase activity, ATP binding to facilitate tryptophanyl-tRNA aminoacylation.2. The protein design must feature the 'HIGH' region, 'KMSKS' region motif.3. We need a protein that can perform ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-   tryptophanyl-tRNA(Trp) with high efficiency. The designed protein sequence is ",
    "response#1": "MDIKTWIR",
    "response#2": "GSSHHHHHH",
    "response#3": "MVNDMK"
  },
  {
    "reference": "MHWADVIAADLLKRSNSHRIATGISPSGHIHLGNLREMVTADAIRRALLDAGGEAKIVYIADDFDPLRRRYPFLPEEYENYVGMPLCKIPDPEGCHDSYSEHFLQPFLESLEILGIPVEVRRAYQMYSEGLYENNTRIALKRRDEIARIIAEVTGRELEERWYPFMPLCENCGRINSTRVTSFDENWIYYECDCGHSGRVGYVGGGKLTWRVDWAARWQILSITCEPFGKDHAAAGGSYDTGVRIAREIFDYEPPYPVPYEWIHLKGKGAMKSSKGIVLPVREMVEVIPPEIVRYITIRVKPERHIEFDPGLGLLDLVEEFEEKFKEKDRSVELSLVGEVVYSDVPFRHLIVVGQIANWDLEKALEIIERTGYTVDDVTRRDVERRLKYARKWLEKYAPDNIKFEIPEKVTAEFSEEEKKFLRAYAERLRSDMKPEEIHTLVYDVSKEVGIKSSKAFQAIYKAILGKTYGPRVGYFIKSLGVEWVRERIKAAL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The ideal protein would be one that plays a critical role in lysyl-tRNA aminoacylation through lysine-tRNA ligase activity, ATP binding within cytoplasm.2. Incorporating a 'HIGH' region motif is critical for the successful design of the protein. The designed protein sequence is ",
    "response#1": "MFPYPSGAGLHVGHVVGYTATDIIARYMRTKGYNVMHPMGWDSFGLPAEQYAIRTGTHPAESTQENINNYRRQLRALGFSYDWNRELATSDPNYYKWTQWIFTKLYEKGLAYEAEMLVNYCPALGTVLANEEIR",
    "response#2": "NPEVRALFRTRARIISYIRSFLDNLEFLEVETPSMNLTAGGASARPFITHHNELDTELLIRVSPELYLKKLVVGGFDRVYELGKHFRNEGMDLTHSPEFTMCELYMAYADYNDLMDLTEQLLSGMVKDLTGSYKIRYHANGLDNEPIEIDFTPPFRKIDMLSELEKVANISIPRDLSSESANKHLVDVCEKFDVKCPPPHTTTRLLDKLVGHFIEVNCINPTFIINHPEIMSPLAKSRRSEPGLTERFNLFVNRRELCDAYTELNDPTAQRERFAEQLKDRQLGDDEAMDLDESFITALEYGLPPTGGLGMGIDRLTMLLTDSQNVKEVILFPAMR",
    "response#3": "GLLERYGQDDSLGYFATRRDKAVIFAPNGRAAITYRVEVGVCLVSADPIGDKAAWGQAISAWLQVCQAYGWAPAVMGASAEGALAYREHGLSALELGDEAILYPRSFSISGPHMAAVRQAVNRARRSGLSVRIRRHRELGAEETKQVIANADLWRDGNTERGFSMALGRLGDSTDGDCLLVEAVDPDGNAVAMLSLVPWGSNGVSLELMRRSRQSPNGTIEFMVTELLTRAEGMGIARVSLNFVMFRSVYADGSRIGAGPVLRLWRSVLMFASRFWQFEQLYRSNVKYQPEWVPRYSCWRWVPPFFGWLVGILAILNFLSSISPFIRVMTRMPREFVDEYLFGWPDSSLSWAFVLSLLAAALAVRKSIAWWFLLVNLTVLSALNVGGYIEHRDTIELLGLIVHGGIIVLLLLARREFYAKVRRGATVKAVAVLIVGMAVGTLAAWALLELFPHTLVPGDRFWWALNRVAGFSIASHRDFDGVPPHFLNWLFGLFGALALILTAIVLWARG"
  },
  {
    "reference": "MVGWVCIFLVVLTTATAGLTRNLYELKIECPHTVGLGQGYVTGSVEITPILLTQVADLKIESSCNFDLHVPATTTQKYNQVDWTKKSSTTESTNAGATTFEAKTKEINLKGTCNIPPTTFEAAYKSRKTVICYDLACNQTHCLPTVHLIAPVQTCMSVRSCMIGLLSSRIQVIYEKTYCVTGQLIEGLCFIPTHTIALTQPGHTYDTMTLPVTCFLVAKKLGTQLKLAVELEKLITGVSCTENSFQGYYICFIGKHSEPLFVPTMEDYRSAELFTRMVLNPRGEDHDPDQNGQGLMRIAGPVTAKVPSTETTETMQGIAFAGAPMYSSFSTLVRKADPEYVFSPGIIAESNHSVCDKKTVPLTWTGFLAVSGEIEKITGCTVFCTLAGPGASCEAYSETGIFNISSPTCLVNKVQKFRGSEQRINFMCQRVDQDVVVYCNGQKKVILTKTLVIGQCIYTFTSLFSLIPGVAHSLAVELCVPGLHGWATTALLITFCFGWLLIPAVTLIILKILRLLTFSCSHYSTESKFKVILERVKVEYQKTMGSMVCDICHHECETAKELETHKKSCPEGQCPYCMTITESTESALQAHFAICKLTNRFQENLKKSLKRPEVRKGCYRTLGVFRYKSRCYVGLVWGILLTTELIIWAASADTPLMESGWSDTAHGVGIIPMKTDLELDFALASSSSYSYRRKLVNPANQEETLPFHFQLDKQVVHAEIQNLGHWMDGTFNIKTAFHCYGECKKYAYPWQTAKCFFEKDYQYETSWG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should be able to enhance the efficiency of suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity. The designed protein sequence is ",
    "response#1": "EVKNISTGYMVTNDCTNDSITWQLQAAVLHVPGCVPCEKVGNASQCWIPVSPNVAVQRPGALTQGLRTHIDMVVMSATLCSALYVGDLCGGVMLAAQMFIVSPQHHWFVQDCNCSIYPGTITGHRMAWDMMMNWSPTATMILAYAMRVPEVIIDIISGAHWGVMFGLAYFSMQGAWAKVVVILLLAAGVL",
    "response#2": "EVKNISTGYMVTNDCTNDSITWQLQAAVLHVPGCVPCEKVGNASQCWIPVSPNVAVQRPGALTQGLRTHIDMVVMSATLCSALYVGDLCGGVMLAAQMFIVSPQHHWFVQDCNCSIYPGTITGHRMAWDMMMNWSPTATMILAYAMRVPEVIIDIISGAHWGVMFGLAYFSMQGAWAKVVVILLLAAGVL",
    "response#3": "EVKNISTGYMVTNDCTNDSITWQLQAAVLHVPGCVPCEKVGNASQCWIPVSPNVAVQRPGALTQGLRTHIDMVVMSATLCSALYVGDLCGGVMLAAQMFIVSPQHHWFVQDCNCSIYPGTITGHRMAWDMMMNWSPTATMILAYAMRVPEVIIDIISGAHWGVMFGLAYFSMQGAWAKVVVILLLAAGVL"
  },
  {
    "reference": "MRVVVGVGGGIAAYKSCSLIRSFTENGHHVRVVPTESALEFVGRATFEALSGSPVHTGVFADVPEVPHVRLGQEADLVVVAPATADLLARLAGGRADDLLSATLLTARCPVLLAPAMHTEMWEHPATAANVDTLRSRGVVVLEPASGRLTGRDTGSGRLPEPDEIFGLAMLLVERADALPRDLVGRKVVVTAGGTREPLDPVRFLGNRSSGKQGYALARVAAQRGADVTLIAGFTTELPDPAAVDVVHVKTAQQMQTAVRKFAPEADAIVMAAAVADFRPATIAGSKIKKGANEPDSIPLVRNDDILAGLVDARRSGSIETSTVIVGFAAETGDEHGDVLTYARAKLARKGCDLLVVNAVGDGKAFEVDHNNGWLLGSDGSESALGEGSKALLAGRVLDAVVAVLDSTSNQQ",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.2. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A3. The designed protein should have phosphopantothenoylcysteine decarboxylase activity to facilitate coenzyme A biosynthetic process.4. Mg(2+) is required for the proper folding and stability of the protein.5. The protein contains novel Flavoprotein domains that confer a unique function or activity.6. A protein should be able to manipulate the activity of enzymes in the Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "MRVVLGVSGGIAAYKACSLLRLFTEHGHDVTVVPTAAALQFVGAPTWSALSGKPVSTEVWTSVHEVPHVRIGQAADLVVVAPATADLLAKAAHGLADDLLTNTLLTARCPVAFAPAMHTEMWEHPATQANVATLRSRGAHVIEPAVGRLTGKDSGAGRLPEPEALYAVVEAALRPRALAGREVVVTAGPTHEAIDAVRYLANASTGKMGYAIAGELAARGALVTLVSGPCALATPRGVRRVDVVSAAQMHDATLAAFEGADAVVCTAAVADYTPAHPADHKLKKSHEHLDAIELVETADILAELGRTKHGRTVVGFAAETDDLIANAQAKLARKHADLIVANDVSRADSTFGADTSRIALVDEAGVEDWPLMGKDAVARRLAVRIAEKLG",
    "response#2": "MRVVLGVSGGIAAYKACSLLRLFTEHGHDVTVVPTAAALQFVGAPTWSALSGKPVSTEVWTSVHEVPHVRIGQAADLVVVAPATADLLAKAAHGLADDLLTNTLLTARCPVAFAPAMHTEMWEHPATQANVATLRSRGAHVIEPAVGRLTGKDSGAGRLPEPEALYAMVEAALAPKALAGKTALVSAGPTQEPIDPVRFISNHSSGKMGIALAEALAEAGAKVQLVLGPTPLSTGHAGVSITRVVTAAEMYDNCLRLSPGTDIMVMAAAVADYRPRTVADIKIKKKDDDMSIALERTADIARTLGEQKKNGQLLIGFSLETNNEREYALKKLKDKHMDMVVLNSLNDAGAGFNHDTNKVTLLFADGAARDLPLASKTELARELVRRIAELLPA",
    "response#3": "KRILLGLTGGIAAYKSAELARSLVRAGCDVRAVLTEAATHFVGVATLQALTGQTAWSDLWDPRVADNMAHIELSRDRDLIVVAPASADFIAKAAHGLADDLLTTLVLARRCPLLVAPAMNVEMWDNPATQRNLLALRDDGVAIAGPVAGDQACGETGMGRMLEPAGILAAAIAHEFFRPRAALTGIKLLMTAGPTYEAIDAVRGITNRSSGKMGYAIAQAAREMGAQVTLVSGPVALTTPVGVERISVNSAQEMYDAVNRRAGDADIFIGVAAVADYRVAHPAEQKIKKSAAPMTLELVANPDILADIASLPNPPFCVGFAAESERLAEYAEQKRRAKKLPLLVGNLAQQAIGSDDNELIAWFDDSGSHGLPRMPKDELARRLVAEIARRLDD"
  },
  {
    "reference": "MLAGIGFYRRFISPLIGPRCRFTPTCSAYGLEAIQRHGPWKGGWLTVKRLLRCHPFTPCGCDPVPD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a specific function within the plasma membrane. The designed protein sequence is ",
    "response#1": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPV",
    "response#2": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPC",
    "response#3": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPV"
  },
  {
    "reference": "MWGGRFNEQVDALMAQFNNSFSFDRRMWREDIRGSIAWARQLARVGVISAEERDTLVDGLNMVYAEFADGRFEARETDEDIHTAVERRLGELVGTVAGKLHTGRSRNDQVATDVRLWTLGAIRRIDDRLRALQTALLAQAETAGAALMPGYTHLQRAQPVLLAHWLLAHFWPLQRDRERLADCAKRTATLPLGSGAIAGTPLMVDRTALAVELGMTAVSPNSIDAVSDRDFIAEFLFCAALIGTHLSRLAEDMIIYSSAEFGFVTLADAYSTGSSLMPQKKNPDSFELLRGKAGRLTGDLIAVLTMLKGLPSAYDKDLQEDKEPLFDAADTLELALPVAAGAVATARFHPDRMRAALDDAMLATDLADYLVARGVPFREAHHIVGRLVREAEQRGVALSALPLEVFLAAHPVCEPDVLQVFDFDRSVAMRRVPGATAPEAVNEQIAQARRCVEER",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should interact with enzymes and substrates within the Amino-acid biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "ESPVELTERLSDRLGVNFYVKREDRQRVFSFKLRGPYNMMSSLSHEEIDKGVITASAGNHAQGVPFPFPGRRLKCVAKIVMPTTTPNIKLDGVRALGADVVLWGHTFDEAKTHAVELCEKDGLRTIPPFEDPAVIKGQGTIGSEINRQIKRIDAVFVPVGGGGLIAGVAAFFKQIAPQTKIIVVEPYDAASMALSVHAEHRAKLSNVDTFADGATVAVIGEYTFARCQDVVDAMVLVANDGIGAAIKDVFDEGRNIVETSGAAGIAGMYCEMYRIKNDNMVGIVSR",
    "response#2": "IIHPIDKPYSEVGGLAILFGNLAEEGAVVKTAGIDPNMREFTGKAICFDSQQEAIDGILGGKVKPGHVVVIRYEGPKGGPGMQEMLAPTSLIAGMNLGDKVALITDGRFSGATRGASIGHVSPEAAEGGVIGLLQDGDEIYINVDTYTLEVKLSDEELEERRKNFKPKVKDIKGRWLRQYRSLVTNAANGC",
    "response#3": "EVIHTLDNPYSEHGGLSMLHGNIAPLGGVIKVGGVDPSIQTFTGAAICFDSHDAAVAAIDNHTVRPGHVVVIRYEGPKGGPGMPEMLAPTSSIVGRGLGKEVALITDGRFSGATRGIAVGHISPEAAALGPIALIEDGDMVTIDLVHRTLNVDVTDDELKQRKARLQPFKAKVKSGYLARYTALVTSANTG"
  },
  {
    "reference": "MDYSGVYPSSSKVYVRGNLYSDVNVGMRKVAIKNGTSEFLVYDTGGPYTDREAKLDIKSGIRKLRSTWIENRRDTSPVQRSFVPSGSSCTRYQKAAENVLRKIAGGSPVTQLFYAQNGIITPEMEYVAIRENALRMQVGSSETGAAAHQEITPEFVRQEIAAGRAIIPANINHPESEPMVIGRNFLVKINANIGNSAVLSGIEEEVKKMALAVAYGADTVMDLSTGSDIHNIREWIIRNSPVPIGTVPIYQALNKVKGVVENLSFEVFKETIIEQAEQGVDYFTIHAGVLKDYIKHARGRVTGIVSRGGAIMAQWCLAHNKENFLYTHFDEICEIMGSYDVAFSLGDGLRPGSIDDANDEAQFLELKTLGELVKVAWHHGCQVMVEGPGHVPMHLIKENMEKQLHLCSEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNIDDVKAGVISYKIAAHAADLAKGNPSAYARDYALSQARFDFRWYDQFNLSLDPLTAKSLHDESLPGKGGKTANFCSMCGPKFCSMKLSKELVDQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a fast turnover rate for 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L-   methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-   deoxyadenosine + CO + formate + 3 H(+) + L-methionine.2. The protein must be able to zinc ion binding.3. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+), substrate binding. The designed protein sequence is ",
    "response#1": "MLAYMNRESIKRTLDSGTTWFWSRSRQEYWNKGATSGHYQHVVSITADCDDDALLIRVKQDGAACHTGSYSCFQ",
    "response#2": "MLAWMNREALQKTAELGRAVYFSRSRNKLWFKGEESGHVQIVHEIRIDCDQDVILLKVTQTGHTPGIACHTGRHSCFL",
    "response#3": "MLAYMNRESIKRTLDSGTTWFWSRSRQEYWNKGATSGHYQHVVSITADCDDDALLIRVKQDGAACHTGSYSCFL"
  },
  {
    "reference": "MQLYFATGNQHKAQEVEAILRARGADVEILMHKGEEPVESGSTFLENALIKARAAFAATGAPSFADDSGIAVEVMGGAPGIFSAIWSGGRDDKTNRDLLLAQLIDIPEENRAAAFVCTMALVDDAGEVSVTGVWPGKIATQASGEHGFGYDPIFIPQGFSVTAAELEPEVKNSFSHRAMALQQLASLLTSR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must be able to perform dITP diphosphatase activity, metal ion binding under specific conditions. The designed protein sequence is ",
    "response#1": "MPIGVPKVPFRSPGAND",
    "response#2": "GFAA",
    "response#3": "MDL"
  },
  {
    "reference": "MTLKLYDTMAREKRDFVPADPGRVTMYVCGPTVYNHAHIGNFRPVVVFDVLFRLLRHVYGEDAVVYARNVTDVDDKINAKATAEGVEIKVITDRYLAAYHEDAAALLTLPPSLEPKATEHMGPIIEMIGELVANGSAYAAEGHVLFDTQAFPDYGQLSGRDLDDMIAGARVEVAPYKRHPADFVLWKPSKENEPEWDSPWGAGRPGWHIECSAMIDKTLGQTIDIHGGGIDLAFPHHENELAQSRCAHGAPVLANYWLHNGFLDMAGEKMSKSLGNVIIPHELLKTTPGEAIRWALLSGHYRQPLDWTPELIEQSKKALDRLYGALRRAKTVEAGESEPSDEVMAALSDDLNTPLAVSGFFELSSAIERAVTAGDELAITANKGRLLASAGLLGFLQADPDSWFEGDADDDLKAKVEDLLARRIAARTAKDWTAADAIRAEIDALGVVVMDGPAGATWRMKD",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have a binding site that is specific to the target Zn(2+) ligand. The designed protein sequence is ",
    "response#1": "MLAYATREAVARTATTGRAHYYSRSRDELWEKGATSGNTQSVDEIRVDCDGDALLYLVAQTGGACHTGHESCFN",
    "response#2": "MLAYATREAVARTATTGRAHYYSRSRDELWEKGATSGNTQSVDEIRVDCDGDALLYLVAQTGGACHTGHESCFR",
    "response#3": "MLAYATREAVARTATTGRAHYYSRSRDELWEKGATSGNTQSVDEIRVDCDGDALLYLVAQTGGACHTGHESCFL"
  },
  {
    "reference": "MSSLSCGTAVCVVCLEKPKYRCPACRVPYCSLPCFRKHKAPPLQQLPVCPLRLGACCCFIEQCRPAAGPVEKKIRSALTAKTKKPVENEGSLDDDDSVADFLNSDEEEDRVSLQNLKNLGESAALRSLLLNPHLRQLMVDLDQADDKAKLMRACMQEPLFVEFADCCLSIVEPSQNEDP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that can perform metal ion binding in nucleus, pre-snoRNP complex, cytoplasm to support box C/D snoRNP assembly, maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA), snoRNA localization.2. The designed protein should have a binding site that is specific to the target Zn(2+) ligand. The designed protein sequence is ",
    "response#1": "PCPHCAGTGNVRSESSIALHVIRSIEEYLLHNPHYHIIVRTPVTTALYVLNHKRNILVNLEERFKLTISIEADDSIGTQHLIISC",
    "response#2": "GQQSSDIIQKLQKDLFMLKKPEAIQYNKKNAFRPMESAESIEFLGEKSNSSLFTFGSHSKKREHNLIMGRLFDNAVFDMIEMGISNFKSIKEFGADGSAVGNKPMFIFQGNEFENNETVKKVGNLFLDMFRGRLVEYINLVGLDHVVIMTAVDDKILFRHYTTIFSPSGSKIPKVDLKEVGPSFDITIRR",
    "response#3": "GQQSSDIIQKLQKDLFMLKKPEAIQYNKKNAFRPMESAESIEFLGEKSNSSLFTFGSHSKKREHNLIMGRLFDNAVFDMIEMGISNFKSIKEFGADGSAVGNKPMFIFQGNEFENNETVKKVGNLFLDMFRGRLVEYINLVGLDHVVIMTAVDDKILFRHYTTIFSPSGSKIPKVDLKEVGPSFDITIRL"
  },
  {
    "reference": "MKTAEKLGIIGTTISIFGIGFGWGVFPWLIRMQIGRVSLSPGSETREFWEKIPFPIDFKIHIFNITNHVEVQNEGKIPNLQEIGPYYYKEWKEKSEMIDYENDDSITFFMKNTWFDNKEKTLPLTGDEMVIIPNPILVGLITAAEREKKGVLPMINKAIPILFNKPDSVFLKIKVYDLLFGGIIFNCTTKDFSASAVCAVLKKEAPFLETVSRSVFKFSILNQKNGTEEPLKLQIKRGIKDYTEVGKVIGANGKNKLTNWRGRPCNNLEGTDGTIFPSDISEHQDIWSFNLELCRSIPAKFVRKSEYKGIPAFRYNVTIGDTSTDPSLKCLCINDTFCWKKGAMELLKCSGLPVVATLPHFYDSHEDFLNGVKGLSPNEENHSIFFDIEPMTGTPLYAKKRIQFSFPLGKINKIDLTKNLPDTLLPFLWVEESIELPDYLIDKLNSELFRILQFLDVIKWVITLFGAGVVSGGVGLYYKEKNSLPITPTSSATSKKIDNPTDKTTTHELGHTNFGYIN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should contain Extracellular topological domains.2. The protein design should be able to enhance the efficiency of sensory perception of smell. The designed protein sequence is ",
    "response#1": "HDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQQDNCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLDLYRQTDRIMAEFF",
    "response#2": "VKTDQEELLAQELEDLNKWGLNIFHVSDYARGRSLSCIMYTIFQERDLLKKFHIPVDTMVTYMLTLEDHYHQDVAYHNSLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQQDNCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLDLYRQTDRIMAEFFQQGDASGRGDGDQPHVRQHTARGESQVGFIDYIVHPLWETWADLVHPDAQEILDTLEDNRDWYYSAIRQR",
    "response#3": "VKTDQEELLAQELEDLNKWGLNIFHVSDYARGRSLSCIMYTIFQERDLLKKFHIPVDTMVTYMLTLEDHYHQDVAYHNSLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQQDNCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLDLYRQTDRIMAEFFQQGDASGRGDGDQPHVRQHTARGESQVGFIDYIVHPLWETWADLVHPDAQEILDTLEDNRDWYYSAIRQC"
  },
  {
    "reference": "MSSFLTSERAGHEQHHAEARRLRLFSGTANPALSREIAAYLGVPDGPRVCKRFADGELYVQIQESIRGCDVFLIQPTCAPVNDNLMELLIMVDACRRASARQITAVVPYYGYARADRKTAGRESITAKLTANLLVNSGVDRVLAMDLHSAQIQGYFDIPCDHIYGSPVLVDDLCSRDLGDLVVVSPDVGGVARARAFAKQMNGAPLAIIDKRRSGHNVAESLTVIGEVAGRTAVLIDDMIDTGGTICQGARLLRQEGAARVIACASHAVFSPPAMERLSEPGLFEQVLVTNSVPLPEERRFPQLKVLSVAKMLGEAIWRIHEESSVSSMFR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be able to modulate phosphorylation in a way that leads to a desirable outcome.2. The designed protein must have a active site that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "LFREEFNALPVCPIQASCDAASKEENKEKNRYVNILPYDHSRVHLTSLEGVPDSDYINASYINGYQEKNKFIAAQGPKEETVNDYWRMIWEQNTATIVMVTNLKERKECKCAQYWPDQGCWTYGNIRVSVEDTMILVDYTIRKFCIQQVGDVSGKKPQRLVTQFHFTSWPDFGVPFTPIGMLKFLKKVKNCNPQYAGPIMVHCSAGVGRTGTFIVIDAMLDMMGAERKVDVFGFVTRIRAQRCQMVQTDMQYVFIFQALLEHYQ",
    "response#2": "NMKKNRVLQIIPYEFNRVIIPVKRGEENTDYINASFIDGYRQKDSYMACQGPLQHTIEDYWRMIWEWRSCSIVMLTELEERGQEKCAQYWPTDGVMVCGDMSIELKREEESESYTVRDLLVTNNRENKSRAVRQFHFHGWPEVGIPSDGKGMINIIAAVQKQQQQSGNHPITVHCSAGAGRC",
    "response#3": "FREEFNALPVCPIQASCDAASKEENKEKNRYVNILPYDHSRVHLTSLEGVPDSDYINASYINGYQEKNKFIAAQGPKEETVNDYWRMIWEQNTATIVMVTNLKERKECKCAQYWPDQGCWTYGNIRVSVEDTMILVDYTIRKFCIQQVGDVSGKKPQRLVTQFHFTSWPDFGVPFTPIGMLKFLKKVKNCNPQYAGPIMVHCSAGVGRTGTFIVIDAMLDMMGAERKVDVFGFVTRIRAQRCQMVQTDMQYVFIFQALLECC"
  },
  {
    "reference": "MKDLQKLSPEIVTINQSFEMEGADMDKMLIFFYEDLRAIGDSWIMDSDWIYRSKYKNSGVGKNKSDRLVEHVLAALDGLIKTTRERFGMMDLESEGRKSFTTPKGVSSEARRSFTRSASYSESNNSFYPSPLTPRSVLPGTMMMSSNSTSPSLWNLRAQALDRLSPVDLKRFAMQILSQRDSESVSETKIGIEEENEESEILAEEKEEEDNDFSVLETEGTEQEIKTEHHRESSGTEHETEAKDHSEGFETEHHRIECFETEHEIDADDHIEDFETEHHHIEGLETEHEIDANDHIEDFETEHHHIEGFETEHENETEDHSETTTSETDSTESSPKEDVPPPPPLTSPQTPSPTVSTFNTKSSLRSQPPPPPPSPEHKAPAPPPPPPMSKASESGEFCQFSKTHSTNGDNAPSMPAPPAPPGSGRSLKKATSKLRRSAQIANLYWALKGKLEGRGVEGKTKKASKGQNSVAEKSPVKVARSGMADALAEMTKRSSYFQQIEEDVQKYAKSIEELKSSIHSFQTKDMKELLEFHSKVESILEKLTDETQVLARFEGFPEKKLEVIRTAGALYKKLDGILVELKNWKIEPPLNDLLDKIERYFNKFKGEIETVERTKDEDAKMFKRYNINIDFEVLVQVKETMVDVSSNCMELALKERREANEEAKNGEESKMKEERAKRLWRAFQFAFKVYTFAGGHDERADCLTRQLAHEIQTDPDQSESSIMS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design should emphasize Pro residues compositional bias.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in chloroplast outer membrane that is critical for chloroplast organization.3. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "ELQRYTDSNNGFTLLIPSSYTKVEKAGANALFEELNNGSNNIGVVVSPVRIKSLDQFGSPQFVADKLINAEKRKESTKEAEVVSVGERAGLGQQVYEFEYKIDSTRGGIKRVFSAAFVSSNKLYLLNVVHSDKPENPLDSSTRMSLEQVLHSFR",
    "response#2": "ELQRYTDSNNGFTLLIPSSYTKVEKAGANALFEELNNGSNNIGVVVSPVRIKSLDQFGSPQFVADKLINAEKRKESTKEAEVVSVGERAGLGQQVYEFEYKIDSTRGGIKRVFSAAFVSSNKLYLLNVVHSDKPENPLDSSTRMSLEQVLHSFR",
    "response#3": "ELQRYTDSNNGFTLLIPSSYTKVEKAGANALFEELNNGSNNIGVVVSPVRIKSLDQFGSPQFVADKLINAEKRKESTKEAEVVSVGERAGLGQQVYEFEYKIDSTRGGIKRVFSAAFVSSNKLYLLNVVHSDKPENPLDSSTRMSLEQVLHSFR"
  },
  {
    "reference": "MRRLTRRLVLPVFGVLWITVLLFFWVTKRKLEVPTGPEVQTPKPSDADWDDLWDQFDERRYLNAKKWRVGDDPYKLYAFNQRESERISSNRAIPDTRHLRCTLLVYCTDLPPTSIIITFHNEARSTLLRTIRSVLNRTPTHLIREIILVDDFSNDPDDCKQLIKLPKVKCLRNNERQGLVRSRIRGADIAQGTTLTFLDSHCEVNRDWLQPLLHRVKEDYTRVVCPVIDIINLDTFTYIESASELRGGFDWSLHFQWEQLSPEQKARRLDPTEPIRTPIIAGGLFVIDKAWFDYLGKYDMDMDIWGGENFEISFRVWMCGGSLEIVPCSRVGHVFRKKHPYVFPDGNANTYIKNTKRTAEVWMDEYKQYYYAARPFALERPFGNVESRLDLRKNLRCQSFKWYLENIYPELSIPKESSIQKGNIRQRQKCLESQRQNNQETPNLKLSPCAKVKGEDAKSQVWAFTYTQQILQEELCLSVITLFPGAPVVLVLCKNGDDRQQWTKTGSHIEHIASHLCLDTDMFGDGTENGKEIVVNPCESSLMSQHWDMVSS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein have Ricin B-type lectin domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "YPFAAKSLFKGSNNIIPYYLSTSNTFQCVASESIQTWLLSDDGHIFTSSGNFVLDVSSGGYFVELVQLNSNSKTQIWTIDTTNNKIQNQGNGKYLDIDNLNICVAPLNGNATQKWTTC",
    "response#2": "TTIVGLYGLCLQANSGQVWIEDCSSEKAEQQWALYADGSIRPQQNRDNCLTSDSNIRETVVKILSCGPASSGQRWMFKNDGTILNLYSGLVLDVRASDPSLKQIILYPLHGDPNQIC",
    "response#3": "YPFAAKSLFKGSNNIIPYYLSTSNTFQCVASESIQTWLLSDDGHIFTSSGNFVLDVSSGGYFVELVQLNSNSKTQIWTIDTTNNKIQNQGNGKYLDIDNLNICVAPLNGNATQKWTTC"
  },
  {
    "reference": "MEPSLIDVRRALHRIPELGFEEYKTQTYLLDLIQSLPQDFLEVKTWKTGILVRVGGRKGEKTVAYRADMDGLPITEETGLPFVSQHEGRMHACGHDLHMTIAFGLLRHFAYHQPETHLLFIFQPAEEGPGGAKPMLDSEEFRMWWPDEIIALHIAPEYPVGTIATRKGLLFANTSELFIDLKGQGGHAAYPHLANDMVVAASHLVTQLQSVVSRNVDPLDSAVVTIGVIKGGTKQNIIAETARIEGTIRTLSIESMKKVKKRIEALVSGIEIGFSCQASIDYGSNYCQVWNDEERVARFIEYSQGREGVTFIECSEAMTGEDFGYFLEEIPGFMFWLGVDTNYGLHDARLNPNEDVLSFAVQHLIGYIETL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Catalyzes the conversion of N-acetyl-diaminopimelate to diaminopimelate and acetate.2. The protein with the reaction H2O + N-acetyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)-2,6-   diaminoheptanedioate + acetate, the Proton acceptor, active site must be optimized to ensure efficient catalysis.3. The protein should be optimized for activity in Amino-acid biosynthesis, L-lysine biosynthesis via DAP pathway, LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (acetylase route): step 3/3 pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The protein design must be able to perform N-acetyldiaminopimelate deacetylase activity in order to contribute to diaminopimelate biosynthetic process, lysine biosynthetic process via diaminopimelate. The designed protein sequence is ",
    "response#1": "MRYLKNSHIKKLGLTGNEDSLVADVSFDSRRVKSGGLFVAVPGFSNDARKYINDAIDKGASVIISEEKLPIENIPQVIVPDLRKMLAQLSAYFYDHPSKELKIIGT",
    "response#2": "MKLKQLLSAMPFVQESGNFDVXVXGKTXDXRXGXGXIXGXDXDXNXALXXGXAXCXTTXSXLDXFXGXIAGXNXPXAXVXDXKLXAFXASXFNXPSTXXVXGXIXPXVXXKXIVGTCEVFITFKGKGGHAAFPHNANDALVAASYFITQVQTIVSRNVDPIEGGVVTFGSMHAGTTNNVIAETATLHGTIRTLTQDMSLLIQKRVQEMGVTXXQXVVXGXSXIPQXVIAGTCEVFITFKGKGGHAAFPHNANDALVAASYFITQVQTIVSRNVDPIEGGVVTFGSMHAGTTNNVIAETATLHGTIRTLTQDMSLLIQKRVQEMSXNXXXGXAXSXDXXSXXXQXDXXXA",
    "response#3": "MSLGTCEVFITFKGKGGHAAFPHNANDALVAASYFITQVQTIVSRNVDPIEGGVVTFGSMHAGTTNNVIAETATLHGTIRTLTQDMSLLIQKRVQEMLNWFESPDKKLNILGICAHIDHGKSTLVDQILFDLKKTINPLERKMTQILDNEELERQIRRSISMSVVPIYFKYKDKYLNLIDTPGHVDFTGNVTRALRLIDGSILVVDALEGVMPLTQKVFNQSITEKLKPIIFINKIDRLINELKLNPFEIQNRILKVIDELNSFIDNYKDIEDIKIEFKDKKIVFFASLLDGYGLNIKDLIKLIKNTDYNKNLSKYDNFEDLTKKVLENFIKPIPLKPIKFIFNKFSLDSNKNIPNKLTVKIYKNNDLNIDFQGKGGHAAYPHTANDMVVACAHFVTQVQTVVARNIDPLDSAVVTLGVIAGGTKQNVIAATARLEGTIRTLSMASMEVVKSRLEAIATKEIKIVYKESL"
  },
  {
    "reference": "MAPRFSWSLATSWHALAILALWPASTLAGDKSAADYYVRELPGLPKNSPPIKMHAGHIEVTPETNGNLFFWHFQNNHIANRQRTVIWLNGGPGCSSEDGALMEVGPYRVTKDNALTLNNGTWNEFANLLFVDNPVGTGFSYVDTNSYIHGLNAMATQFITFLEKFFALFPEYQSDDLYIAGESYAGQHIPYIARAILDRNKSKSRAETWNLGGLLIGNGWISPQDQSSAYLKFSLERGLIEKGSDNAQQLQQMQRICDKEMSINPGHVDYPECESILNKILELTRVGSGDQECINMYDVRLRDSAPSCGMNWPPDLKYVGPYLRQPQVISALNLDKQRNTGWQECNSMVNANFRNQNATASISLLPDILKEVPILLFSGAEDLICNHVGTEELISNLAWNEGKGFEVTPGNWAPRRQWTFEGEVAGFWQEARNLTYVLFHNASHMVPFDYPRRSRDMLDRFMKVDISSIGGEPSDSRIDGEKGPDTSVGGAKNNTQQHEEETKQKLKEAQWLAYQRSGEVVLVIVIIAASVWGYFVWRQRRKGTAYSALQSDEAAGQSRTGLAAFHNRQSDRDLEAAAFDETTVDNIPLQESIGRGESKYSIGDDSDEEEGETNKT",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Incorporate a signal peptide in the protein design.2. The designed protein should contain Lumenal, Cytoplasmic topological domains.3. The designed protein should have at least Helical transmembrane domains to ensure proper localization.4. The protein must exhibit the following characteristics: Promotes cell fusion and is involved in the programmed cell death .; Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from protein precursors.5. A protein that can perform serine-type carboxypeptidase activity in membrane to support apoptotic process, proteolysis.6. A key design requirement for the protein is the optimization of the active site to achieve high catalytic efficiency for Preferential release of a C-terminal arginine or lysine   residue.. The designed protein sequence is ",
    "response#1": "SPDLKTVLAMTREKKNWRHSFTGLYWLFDVETQTAQPLDPDAPNGRIQLATWSPTSDAVAFTRDNNLYIRNLTSKSVKAITTDGGTNLFYGIPDWVYEEEVFEGNIATWWSLDGKYISYLRTNETLVPEFPIDFYLSSPPGYSPKPGEESYPYVQQIKYPKAGAPNPTVSLQFYDIEREESFSVDVKDTLKDDDRLIVEVIPGSKGKVLVRETNRESYIVKVAVIDANKREGKIVRSDNIDEIDGGWVEPSHTTTYIPADPSAGRPDDGYIDTVIHEGYIHLAYFTPLENPKPKMLTTGKWEVVAAPSGVDLKNNVVYFVATKESPIDRHVYSVKLDGSELRMLKDSDKSAYYDVSFSHGAGYMLLKYQGPQIPELNGHLCRLGSAYCYNGRCQALNDQCVSLFGKGSQGASYACFEKVNSPRENLANCDSKDSYSVPCGQQDVLCGKLACFRPPKNYKSPSQSVVYSYVHDSVCLSILPGLSMRSDGRDSAYVADGTVCGPQMYCINGTCKENGHLCRLGSAYCYNGRCQALNDQCVSLFGKGSQGASYACFEKVNSPRENLANCDSKDSYSVPCGQQDVLCGKLACFRPPKNYKSPSQSVVYSYVHDSVCLSDANGHLCRLGSAYCYNGRCQALNDQCVSLFGKGSQGASYACFEKVNSPRENLANCDSKDSYSVPCGQQDVLCGKLACFRPPKNYKSPSQSVVYSYVHDSVCLSGVMTGNDCNATKKCKGNGICNNFGNCQCFPDYRPPDC",
    "response#2": "MQYVFSVQEHTAGNNVVVAFTIMPQSLNTEEERFPLQFQVFKVPQFQNVQGRLPPAFFSPFRSAAQGTKYVSKCNFTKSFHLNPGTYVVVTTANGKEVEFLLRIP",
    "response#3": "MQYVFSVQEHTAGNNVVVAFTIMPQSLNTEEERFPLQFQVFKVPQFQNVQGRLPPAFFSPFRSAAQGTKYVSKCNFTKSFHLNPGTYVVVTTANGKEVEFLLRIL"
  },
  {
    "reference": "MADVVLDLYAEDLDKDFAGQAQDEFGGDGVDLYDDIGGPTESAASGGGGGGTPSADGAAGPGSGEPGERNSGGPNGVYHQSSGSLTPTMNRRYQLYVGNLTWWTTDQDIANSLRDIGVSDLQEVKFFENRANGQSKGFSVISLGSESSLRAVLDQLPKKEMHGQAPVVTYPSKQALTQFESLQKTRPVPPPQQNGPPRGPAPPSMGGGPMPTGHPGGPQGGGPPGHPPRGMNSIMQPGQYRPQHMSQVPQVGGPNSGPPRMQPPMHPQGGLMGNQQPPPRYPSAQGQWPGQRPGGPRPGPPNGPPQRPMFQGGPMGMPVRGPAGPDWRRPPMHGGFPPQGPPRGLPPAPGPGGPHGAPAPHVNPAFFNQPGGPAQHPGMGGPPHGAPGPQPGMNMPPQQGMNMTPQHGPPPQFAQHGPRGPWPPPQGKPPGPFPDPQQMGPQLTEVEFEEVMSRNRTVSSSAIARAVSDAAAGEYSSAIETLVTAISLIKQSKVAHDERCKILISSLQDTLHGIEAKSYNRRERSRSRERSHRSRQRRERSTSRYRERSRERERDRDRERERDGGSYRERSRSRERERQAPDHYRDDSRSVRPRKSPEPVVAEAAEAPSSKRYYEDRERYRSSDRERRDRDRDRDRERERDRDRREEHRSRH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should contain one or more RRM domains that are essential for its function.2. A protein that can perform mRNA binding in mRNA cleavage and polyadenylation specificity factor complex to support regulation of RNA splicing, mRNA alternative polyadenylation.3. The protein design should emphasize Pro residues compositional bias. The designed protein sequence is ",
    "response#1": "TTRRLHLLTQHELNGTITGLAATRTLESTIDGLDRLIVSFKDAKMALLEWSRGDIATVSLHTYERCSQMNTGDLQSYVPLLRTDPLSRLAVLTLPEDSLAVLPLIQEQSELDPLSEGFSRDAPYSPSFVLSLSDMSITIKNIQDLLFLPGFHSPTIALLFSPMHTWSGRLQTVKDTFCLEIRTFDLSSGTSYPLLTSVSGLPSDSLYLVACPSELGGIVLVTSTGIVHVDQGGRVTAACVNAWWSRITSLKCSMASVSQKLTLEGSRCVFVTPHDMLLVLQNGAVHQVRFSMEGRAVGVIEVLDKGCVVPPPSDLTVAGDGAVFVGSAEGDSWLAKVNVVV",
    "response#2": "TTRRLHLLTQHELNGTITGLAATRTLESTIDGLDRLIVSFKDAKMALLEWSRGDIATVSLHTYERCSQMNTGDLQSYVPLLRTDPLSRLAVLTLPEDSLAVLPLIQEQSELDPLSEGFSRDAPYSPSFVLSLSDMSITIKNIQDLLFLPGFHSPTIALLFSPMHTWSGRLQTVKDTFCLEIRTFDLSSGTSYPLLTSVSGLPSDSLYLVACPSELGGIVLVTSTGIVHVDQGGRVTAACVNAWWSRITSLKCSMASVSQKLTLEGSRCVFVTPHDMLLVLQNGAVHQVRFSMEGRAVGVIEVLDKGCVVPPPSDLTVAGDGAVFVGSAEGDSWLAKVNVVQ",
    "response#3": "TTRRLHLLTQHELNGTITGLAATRTLESTIDGLDRLIVSFKDAKMALLEWSRGDIATVSLHTYERCSQMNTGDLQSYVPLLRTDPLSRLAVLTLPEDSLAVLPLIQEQSELDPLSEGFSRDAPYSPSFVLSLSDMSITIKNIQDLLFLPGFHSPTIALLFSPMHTWSGRLQTVKDTFCLEIRTFDLSSGTSYPLLTSVSGLPSDSLYLVACPSELGGIVLVTSTGIVHVDQGGRVTAACVNAWWSRITSLKCSMASVSQKLTLEGSRCVFVTPHDMLLVLQNGAVHQVRFSMEGRAVGVIEVLDKGCVVPPPSDLTVAGDGAVFVGSAEGDSWLAKVNVVV"
  },
  {
    "reference": "ERVCCGYPMSCKSRACKPSYCC",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Mu-conotoxins block voltage-gated sodium channels (Nav) 2. The protein must have high specificity towards toxin activity. The designed protein sequence is ",
    "response#1": "MH",
    "response#2": "SCGRF",
    "response#3": "GVAH"
  },
  {
    "reference": "MEVLEEDLTCPICCSLFDDPRVLPCSHNFCKKCLDGVLEENSRTMQWRPSSFKCPTCRKETPTMGVNGLQVNYLLKGIVEKYNKIKVSPKMPVCKEHSDQPLNIFCSTDLKLICGSCATTGEHKKHVFSSIGDAYIQEKSSLETLFQGVEEWNSKEVHSHLDTLESNKRKALHSLAKESDKVKAYFEKLQYLLEQKKNEILSDFETLKLAVMQAYDTEINKLHTVLSEQRKACNIVEDLKNISDPFMFLQQMQEFRDKMTFIKEAPLTTGQDVNVNPAMKEFDTSMWDSIKLGEVDKLSLPQDTTSKKEPGDAKTLHSLKPILVVACLILLLVTFLCAYPFIDSLPTFTIDLQVISSYFFTTTAKAANLTILFWEQLSEELLILKQRCQTYVSVFLENVAEFVCKYKL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Ideal protein should have a binding affinity for key enzymes in the Protein modification, protein ubiquitination pathway to enhance the overall efficiency of the pathway.2. The protein must exhibit the following characteristics: E3 ubiquitin ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD); This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins ..3. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects.4. The designed protein should have ligase activity to facilitate protein ubiquitination. The designed protein sequence is ",
    "response#1": "LFAFLNLLPVEADILAYNFENASQTFEDLPARFGYRLPAEGLKGFLINSKPENACEPIVPPPLKDNSSGTFIVLIRRLDCNFDIKVLNAQRAGYKAAIVHNVDSDDLISMGSNDIDILKKIDIPSVFIGESSANSLKDEFTYEKGGHVILVPELSLPE",
    "response#2": "LLSHKMFDPIYCLFEYSAVDNYTLQINPHSSINPEHLNYFRFIGRVIGLAIFHRRFLDAFFVVSLYKKLLRKKVSLADMESIDAEFYRSLKWVLENDITGILDLTFSVEEDHFGEVRTVELITNGENIEVTEENKKKYVDLVTEWRVSKRVEQQFNAFYSGFVELVSPDLVNVFDERELELLIGGISDVDVEDWKSHTEYRTYIATDPVIKWFWEIIAGWKNEDRSKLLQFATGTSRIPVNGFRDLQGSDGPRKFTIEKAGTPDQLPVAHTCFNRLDLPDYPSKC",
    "response#3": "SNFSTIYEPPDLSGLDAEMIVIVKNLQKRDIVTKCRALQDLIQWNDPSQFDNEQFLNALAVLFPRLSIEVERHVRLKIFVFMSVLSSALQKKLAPWLKFYITPWVMGFFDSDRAVSVSAKDSFKNLLSEEKWPHVWLKFGSTIAPIVTDVFLHEDKESLTDLRFFSNEEAESKVTRVKSSCLLTLSFLFG"
  },
  {
    "reference": "MSDRLAAVIVLAAGGGTRMKSAIPKVLHSIGGRSLVGHVLDAARGLQPHFLVVVVGHGREMVEPHVRGLDAHALLAVQTEQRGTGHAVQVALEVLPAGTAGTVIVTYGDVPLQTTHTLAELAARHRAEGNAATILTAHLPDPFGYGRVVRDADSAVAAIVEEKDASPEQRQISEINSGIFAFDLVALRDALGRVRTDNAAGEVYLTDVVSIAVADGRRIGTLTTDDLWQTEGVNTRQQLASLGGELNRRVLDRWMGDGIEIIDPASTWVDVTVELAPDVSLRPGVQLHGNTVVSTGAVIGPDTTLTDVTVGPHAQVVRTHGFGAVLGPKVVVGPFAYVRPGTVIAAGGKIGTFVETKNAVIDEGGKVPHLSYVGDAEIGAGTNIGAGTIFANYDGVNKHHTRIGAHCSTGANNTFVAPVDIGDGASTGAGTVVRRDVPPGALAVSTGSQRHVEGWVERKRPGSSAAKAAGQTRNEEGSP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain..2. A protein that can perform glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity in cytoplasm to support cell morphogenesis.3. We require a protein that can specifically bind to the metabolites produced in the UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway. The designed protein sequence is ",
    "response#1": "LVLAAGAGTRMKSDTPKVLHTLAGRSMLSHSLHAVAKVAPQHLVVVVGRDRERVAPAALEIGQELGRPVEIAVQEQQQGTGHAAECGLSALPDDFTGVVVVTAGDVPLLDADTLADLIATHSAEAAVATVLTTTLADPTGYGRVLRTQDREVIGIVEQADASPSQQAISEVNAGVYAFDIAALRSALSRLRSDNAQHELYLTDVISIVRSDGELLGINERTQLAAAIAEVRRRIAERWMLAGVTVVDPATTWVDVDVELERDVTILPGTQLYGATVVREGATVGPDTTLTSTEVGQHATVSRTQAELAVIGDNASVGPFSYLRPGTVLGSGGKIGGFVETKNATIGDGSKVPHLSYVGDATIGEHTNIGAATIFVNYDGVAKHRSTVGSFSRVGADNLLVAPVHIGDGTYTAAGTVVRGDVPSGALAVSAGPGVTVQDPATTWIDLKATLSPDVTILPNTQIKGATSIATGAVVGPDTTLVDCEIGENATVRRADATLAVVGAGATVGPWAFLRPNTVLDADGKIGTFVETKNSHIGAGSKVPHLSYVGDTEVGVGANVGAGTITANYDGVEKHRTVVGDHVRTGSHNVFVAPVRIGDGAYTGAGTVVRKDVPAGSLAVNVAPVPARPLKNRAERGHHDR",
    "response#2": "VILAAGKGTRMKSRLPKVLHEIAGRPMLGHVLANTRFERDTRTVLVVGPDMDRVSDYARSVRPDIAVVVQQKQLGTGDAARTALPELHDSDGVVLVVFGDTPLVRAETLENMADACRKSGSIVILGFEAADPTGYGRILRDGQGDVLAIVEHKDATPEERAIREINSGIYAFDAAVLRDGLTKIDTANAQGELYLTDVLGLARENGGVTIIDPQTTYIDAGVEIGADTVIWPGTILRGRTVIGEDCVVGPQADITDSVIGRGVTVRHSVLLEAEVGDGCTIGPYAHLRPGSKLAAGCRIGDFVELKNAKLGEGTKVPHLSYVGDAIVGRDVNFGCGAITVNYDGYNKSVTEIGDRAFIGSNVNLIAPVKIGEDAYVVAGSTITHDVKPNDMAIARERQRNIEGWVLRRRPGSKAADVAAAAAEKATAGEDAD",
    "response#3": "MAAGVTLVAPQTVFFSADTEIEADVVVEPNVVFGPGVSIAAGTVIHAFSHVEGARIGADVSIGPFARLRPGTEIEPKARVGNFVETKNLKLAEGAKVNHLTYLGDASVGPRSNIGAGTITCNYDGFNKYRTAIGADAFIGSNSSLVAPIVIGDGAYVGSGSVVTEDVPDEALAIGRGRPVVTEDSLAADGPSGRFKLLVDVEGVAGIETVYIPAGDARDSTVGENTTVGPYAYLRPKSNIGNHVKIGDFVEVDRTKVLHEVAGRALVAHVLASAADAGSDAVALVVGRDAEAVTKLGNALGHPAQSFEQTERLGTAHAVLAARAEIAEGYDDVLVLFGDTPLLSAETLRRARIQLADGAAVCVVGFEADDPAGYGRLLLDGGQLLAIRELPLLGGLVLAAGAGTRMKSDTPKVLHTLAGRSMLSHSLHAVAKVAPQHLVVVVGRDRERVAPAALEIGQELGRPVEIAVQEQQQGTGHAAECGLSALPDDFTGVVVVTAGDVPLLDADTLADLIATHSAEAAVATVLTTTLADPTGYGRVLRTQDREVIGIVEQADASPSQQAISEVNAGVYAFDIAALRSALSRLRSDNAQHELYLTDVISIVRSDGEMLGVDGRTEVARAVLAVILAAGKGKRMKSDVPKVLHKVAGAPLVSHVIDAAREAGIREIVVVVGEKTENLQEILGDGCRYVLQERPLGTGDAVRRALPALTEGAREVVILCGDTPLLRGATIRKLVEERRAASAAVAVLTMAAGVTLVAPQTVFFSADTEIEADVVVEPNVVFGPGVSIAAGTVIHAFSHVEGARIGADVSIGPFARLRPGTEIEPKARVGNFVETKNLKLAEGAKVNHLTYLGDASVGPRSNIGAGTITCNYDGFNKYRTAIGADAFIGSNSSLVAPIVIGDGAYVGSGSVVTEDVPDEALAIGRGR"
  },
  {
    "reference": "MGLTANPPHGGVLKDLLARDAPRHQELSAEAETLPAIVLSERQLCDLELIISGGFSPLEGFMNQKDYDGVVNDLHLADGKLFSMPICLDVTKESLEAKKIKPGARITLRDPRDDSNLAIITVDDVYQPDKNKEAQEVLGGDPEHPAIKYLFEKVHDFYVGGKLDAVNRLNHYDYVALRYTPSELRTHFEKLGWSRVVAFQTRNPMHRAHRELTVRAARARQATVLIHPVVGLTKPGDIDHFTRVRVYQALMPRYPSGMAVLGLLPLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGKNSAGKDFYGPYDAQHAVEKYKSELGIEVVDFQQMSYLPDSDEYRPKDQVPAGTKTLDISGTELRRRLRVGADIPEWFSYPEVVRVLRESNPPRATQGFTIFFTGYQNCGKDTIARALQVTLNQQGGRPVSMLLGEIVRAELSSELGFSREDRDENIGRIAFVAAELTKAGAAVIAAPIAPFEQARVEARNQISQYGSFFLVHVATSLEYCEKTDKRGMYAKARNGEIKGFTGVDDPYEAPTDPDLTVDFEHQSVRNIVHQIVLMLESQGFLEQL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should contain one or more ATP-sulfurylase PUA-like domains that are essential for its function.2. A protein that can perform sulfate adenylyltransferase (ATP) activity, ATP binding in cytoplasm to support hydrogen sulfide biosynthetic process, sulfate assimilation, cysteine biosynthetic process, methionine biosynthetic process.3. The protein must exhibit the following characteristics: Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids.; Catalyzes the first intracellular reaction of sulfate assimilation, forming adenosine-5'-phosphosulfate (APS) from inorganic sulfate and ATP.4. The protein's active site must be carefully engineered to ensure that the amino acids involved in ATP + H(+) + sulfate = adenosine 5'-phosphosulfate +   diphosphate are properly positioned for optimal catalysis.5. The designed protein should have a binding site that is specific to the target 3'-phosphoadenylyl sulfate, sulfate, ATP ligand.6. The protein design should take into account the physiological conditions of the sulfate assimilation, Sulfur metabolism pathway. The designed protein sequence is ",
    "response#1": "TPHGGQLNDLIIRDAHIKQNLLQESATLPSITLTARQLCDLELILNGGFSPLAGFLNEDDYTSVVNDLRLSSVKGANGKGLLWPIPITLDVNEQTASSYANGSRIVLRDLRDETPLAILTVESIYKPNKAVEAEKVFRGDPEHPAVKYLIETAGDYYIGGSLQGITPNGGVLKDLFARDLPRQAELLAEAEQLPTLTLSERHLCDLELILNGGFSPLEGFMTQKDYESVVSTHRLVDGCLMSMPINLDVDQAQIDELGIKEGAKLALRDLRDDRILAILTVEDVYTPDKVKEAVEVFGSDDDTHPGVKYLFSTAKEFYVGGKLEAVNDLRYSPAELRLHFDKLGWGRVVAFQTRNPMHRAHRELTVRAARARQANVLIHPVVGLTKPGDIDHFTRVRVYQALLPRYPNGMAVLGLLPLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGKNSKGVDFYGPYDAQEAVEKYRDELGIEVVPFQQMTYLPDSDEYKPRDEVEAGVRTLDISGTELRRRLRTGVDIPDWFSYPEVVKVLRTPNGGVLKDLFARDLPRQAELLAEAEQLPTLTLSERHLCDLELILNGGFSPLEGFMTQKDYESVVSTHRLVDGCLMSMPINLDVDQAQIDELGIKEGAKLALRDLRDDRILAILTVEDVYTPDKVKEAVEVFGSDDDTHPGVKYLFSTAKEFYVGGKLEAVNLRYSPAELRLHFDKLGWSRVVAFQTRNPMHRAHRELTVRAARTRQANVLIHPVVGMTKPGDIDHFTRVRVYQALLPRYPNGMAVLGLLPLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGKNSKGEEIYGPYDAQYAVEKYRDELGIEVVPFQMMTYLPDSDEYRPKDEVPKDVRTLDISGTELRRRLKTGGDIPEWFSYPEVVKVLR",
    "response#2": "MGYDGFMTEKDYNGVVKDNRLADGALFSMPITLDVDQATIDEVKIAPGARITLRDFRDDRNLAILTVEDAKEVFGGDEEHPAIQYLYNTAKEFYVGGKLEAINLGVIDYPQATPELRYTPSELRSHFRKLAWRKVVAFQTRNPMHRAHRELTVRAARQRQANVLIHPVVGLTKPGDVDHYTRVRVYEAIMAKYPNGMGHLALLPLAMRMAGPREAVWHAIIRKNFGATHFIVGRDHAGPGKNSQGRDFYGPYDAQELVEKYKDELQIEMVPFQQMTYLPSSDEYQPVDEIPAGVQTLDISGTELRKRLKTGAPIPDWFSYDAVVKTLREGKTLPEVYDKVELRYTPAELRAHFDKLGWAKVVAFQTRNPMHRAHRELTVRAARSHHANVLIHPVVGLTKPGDIDHFTRVRVYKALLPRYPNGMAVLGLLPLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGKNSKGVDFYGPYDAQYAVEKYRDELGIEVVPFQMMTYLPDSDEYAPVDQISKGVRTLNISGTELRSRLRSGREIPEWFSYPEVVKVLRRDEPLRDNLKAEAHTLPDIVLTERQLCDLELIINGGFSPLEGFMTEADYKGVVDNLRLADGTLFPIPITLDVSREDIDRLSISSGSRIALRDPRDDEALAIITVEDVYRPDQVKEAIQVFGADDPAHPSVAYLRNKVKEYYIGGKVQAIDLRYSPAELRLHFDKLGWGRVVAFQTRNPMHRAHRELTVRAARARQANVLIHPVVGLTKPGDIDHFTRVRVYQALLPRYPNGMAVLGLLPLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGKNSKGVDFYGPYDAQEAVEKYRDELGIEVVPFQQMTYLPDSDEYKPRDEVEAGVRTLDISGTELRRRLRTGVDIPDWFSYPEVVKVLR",
    "response#3": "MSTPHGGVLKDLFARDLPRHAELRDESEKLPALVLTERHLCDLELILNGGFSPLEGFMIEKDYNGVVDNNRLADGALFSMPITLDVDQGQLDHLGIKAGARITLRDSRDDRNLAILAVEDVYRPDNTSNRVKEAKEVFGSDDDTHPGVKHLFNTAKEFYVGGKLEAINTPHGGQLNDLIIRDAHIKQNLLQESATLPSITLTARQLCDLELILNGGFSPLAGFLNEDDYTSVVNDLRLSSVKGANGKGLLWPIPITLDVNEQTASSYANGSRIVLRDLRDETPLAILTVESIYKPNKAVEAEKVFRGDPEHPAVKYLIETAGDYYIGGSLQGILRYTPAELRAHFKKLAWRRVVAFQTRNPMHRAHRELTVRAARQRQANVLIHPVVGLTKPGDVDHYTRVRVYEAIMAKYPNGMGHLALLPLAMRMAGPREAVWHAIIRKNFGATHFIVGRDHAGPGKNSQGKDFYGPYDAQDLVMKYHEELQIEMVPFQQMTYLPSTDEYQPADEVPKGVQTLDISGTELRRRLKTGASIPDWFSYDAVVKTLRKEPDATKPELRYTPAELRAHFDKLGWAKVVAFQTRNPMHRAHRELTVRAARSHHANVLIHPVVGLTKPGDIDHFTRVRVYKALLPRYPNGMAVLGLLPLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGKNSKGVDFYGPYDAQYAVEKYRDELGIEVVPFQMMTYLPDSDEYAPVDQISKGVRTLNISGTELRSRLRSGREIPEWFSYPEVVKVLR"
  },
  {
    "reference": "MRLLTSVTALRCYVAQQKRLATNFLHSEEGQLAEVSTWFQTAIGLVPTMGALHEGHVSLIRKARQENRTVIVSIFVNPLQFGPNEDYQRYPRTLEQDAQICEELGVNAIFAPTPEEMGVPLKNIKGSKVTQVTPPSAMISGLCGRSRLGHFHGVATIVTKLFNLVSPDRAYFGEKDFQQLATIQKVVADLHLPVEIVPCPTVRLANGLALSSRNQYLNASEKEQATVIYRSLQKAKNAFCAGEKNSSRLIALVQQELAKVSILSVEYIELVEPTTLMPIEKVKEKGMLAIATRIGSTRLIDNVILRDRQPIVAIDGPAGAGKSTVARQVAAQLELVYLDTGAMYRAMTWLVLQRGITLDDECAIAELADKYEIELTPTSNLQSPVKVWIDGNDVTQVIRTPEVTANVSAIAAQTSVRKSLVRQQKNWGKKGGLVAEGRDIGTHVFPDAEIKIFLTASVKERARRRQLDYETQGLSSINLKQLEKDIAERDWKDSTRTVSPLRKAEDAVELQTDGLSISEVTGKIVYQYQKHLSNL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should contain one or more Cytidylate kinase domains that are essential for its function.2. The designed protein must possess cytidylate kinase activity to support phosphorylation, pyrimidine nucleotide metabolic process under certain conditions.3. The protein design should consider the endogenous proteins and enzymes present in the (R)- pantothenate from (R)-pantoate and beta-alanine: step 1/1, (R)-pantothenate biosynthesis pathway to avoid interference with native metabolic processes.4. The protein must exhibit the following characteristics: Catalyzes the transfer of a phosphate group from ATP to either CMP or dCMP to form CDP or dCDP and ADP, respectively..5. The protein should have a high affinity for ATP, (R)-pantoate. The designed protein sequence is ",
    "response#1": "IAIDGPAGAGKSTVTKQVANKLGLLFLDTGAMYRSVTLAVLREGIDLRDRDKVKAIAAKSKIQLFANPIAGQPMQVLLNGEDITSEIRTPEVTANVSAIAAQAPVREILVKQQQLIGNSGGVVMEGRDIGTHVFPDAEVKVFLTASAQERAKRRHADLIAQGQVAPDLATLEHEIQERDRKDSTRSHAPLIQAEDATLVNTDGLTIEEVVAAIVNLFQM",
    "response#2": "IAIDGPAGAGKSTVTKLVGQNLGLLYLDTGAMYRAVTWMVLQAGVPVEDQAQVAELVSQCQISFNGPENSHVIINGQDVTVEIRSREITNNVSAIAAQPTVRYFMVKQQQKFGTKGGIVAEGRDIGTHVFPNAEVKIFLTASVTERSRRRLLELQQKGQTNISLEEVEKDIIRRDKKDSNREISPLRKASDAMEISTDGLSIAEVTQKIVDIYHPLKT",
    "response#3": "IVAIDGPAGAGKSTVTRAFAERLGLLYLDTGAMYRAVTWWVQEQGADPADEPAVNALLDGLEVDLSPLKNGVQSVRVNGKDVTDAIRDPNVTGSVSLVAAHPCVRQLLTQQQQRLGERGGLVAEGRDIGTAVFPDAELKVFLTATPEERARRRAKDLVARGHAVPDLVELKAQIVERDRLDSTRAVAPLVQAKDATEVITNRGDIGVETLAEVAIDGPAGAGKSTVTRAFAERLGLLYLDTGAMYRAVTWWVQEQGADPADEPAVNALLDGLEVDLSPLKNGVQSVRVNGKDVTDAIRDPNVTGSVSLVAAHPCVRQLLTQQQQRLGERGGLVAEGRDIGTAVFPDAELKVFLTATPEERARRRAKDLVARGHAVPDLVELKAQIVERDRLDSTRAVAPLVQAKDATEVITDGMSIEAVIDALAEVLAG"
  },
  {
    "reference": "MGGKWSKSSIVGWPEVRERIRQTPAAAPAAATGVGAVSQDLDKHGAITSTTTAATNAACAWLEAQEEEEVGFPVRPQVPMIPTTFKAAFDLGFFLKEKGGLDGLIYSKKRQEILDLWVYNTQGFFPDWQNYAPRPGTRYPLTFGWCYKLAPVDPTEVEEEIKEENNCLLHPMCQHGAEDEHKEVLRWKFDSSLARRHAARELHPEFYKDC",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.. The designed protein sequence is ",
    "response#1": "DEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTATDVALGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSYLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKLAKPLSSLTPLIMAAKEAMKSNM",
    "response#2": "DEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTATDVALGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSYLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKLAKPLSSLTPLIMAAKEAMKSNM",
    "response#3": "DEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTATDVALGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSYLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKLAKPLSSLTPLIMAAKEAMKSNM"
  },
  {
    "reference": "MKKVFPENFIGELKEGMASRLSDMMSGYPVVNLLGTLDREITSIAYDSRKVEAGGLFVAIAGTKEDGARYIPEAIRRKAAAFITQSSPQQLLEMGIGINGITQIHVEDTRHALAWLSGRFYNQPSRSLNLIGITGTNGKTTLTYLLESLFSAAGRDCGVIGSINYRYRDTLYPANVTTPESLDLNRMLAEMVTNGVQDCFLEVSSHSLAQKRVNGLHFDLAVFTNLTRDHLDYHSDLHTYRQTKMRLFRDERVEKQVINLDDPMGAKILAETSRPTLTTGIESKADIRAESIELSDHGVRFNLKSPYGSRNIHSPLLGKHNILNLLSSAAVGLFQGVSLDAVCSGIELLSVVPGRLEKIQNNRGFTVVVDFAHTDDALYNALRAVQEFTTGRVVVVFGCGGDRDRTKRGPMGKVAVDNSELAIITSDNPRTENPRQIIEDISLGLPEKAVEEKDYLIIPDRRQAIEKALQLAESGDTVILAGKGAEDYQIIGTEKFHFDDREIVRALLDD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have at least one Mur ligase C-terminal, Mur ligase central, Mur ligase N-terminal catalytic domain.2. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.3. The presence of Mg(2+) is necessary for the protein to function in the desired environment.4. The protein should be designed to have high stability and activity towards ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.5. The designed protein must possess ATP binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, magnesium ion binding to support cell wall organization, cell cycle, regulation of cell shape, cell division under certain conditions.6. The protein design should take into account the physiological conditions of the Cell wall biogenesis, peptidoglycan biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MKLRDLFNGIEIVESKGELDMGEITGLSADSRRIEPGYLFAALPGARADGRAYILDALKRGAAAVLAPLDTELPAEIGRTPVILDQNPRRRLALLAARFYGTPSRRLRMIGVTGTNGKTSVCQYVAQLLKETGEKVGVIGTVNYRYGGREYPSRNTTPESVELQKLFSEMVEEGVEHVVMEVSSHALEQGRVEGCDFDVGVFTNLTQDHLDYHGTMENYFRAKKILFRMLKPGAVAVSNGDDPWCAEILSESQGRVLTYGLGEGADLRAANVGMDLSGIRGEIFYGGRREKVSLPLIGRFNVYNALAAISAGLVLGIPLEEIKGGLERVQGVPGRMERIWSHDRHRCAVVDYAHTPDALQKALEALRTVTPAGAKLAVVFGCGGNRDRQKRPEMGRIAAELADRVILTSDNPRDENPEAILDEIEAGMAGRAHLRIADRAEAIRRAVGQLGEGDILLVAGKGHEDYQILKDGTIHFDEREVVREVVK",
    "response#2": "MKSEITGLSADSRRIEPGYLFAALPGARADGRAYILDALKRGAAAVLAPLDTELPAEIGRTPVILDQNPRRRLALLAARFYGEPSRKLHLIGVTGTNGKTSVCHFTQQLWQMLGFRSTSIGTLGVMGSLEFELKNTTPDPIVLHQILRNLVMNDIDYVSMEASSHGLEQFRLDGVSFKAAAFTNLSRDHLDYHLDEDDYLASKCLLFDRVLSPGNIVVLNLDDPCAQLVKLVSENNGHKVITVGVHDSANLKICDQRYDSDGQTLKISYQGFSQIVRIPLIGNFQATNVLISAAEITGLSADSRRIEPGYLFAALPGARADGRAYILDALKRGAAAVLAPLDTELPAEIGRTPVILDQNPRRRLALLAARFLGVPLDQIADAIHHFGGVKGRMERIWSHDRHRCAVVDYAHTPDALQKALEALRTVTPAGAKLAVVFGCGGNRDRQKRPEMGRIAAELADRVILTSDNPRDENPEAILDEIEAGMAGRAHLRIADRAEAIRRAVGQLGEGDILLVAGKGHETYQIIGDERYPFDDVEEAEVAIRRLRGKVSA",
    "response#3": "MKLKTLLEQVNYKLLEGNLEINGIDSNSKNIKPGYMFIAISGFSSDGHDFIQNAIDNGASVIVAENTDKVKKANIPDLSFLGVEVIKGLNIGVTGTNGKTSVCQYVAQLLKETGKQTGLIGVTGTNGKTTTARLVTSMLNASGKKAGLIGVTGTNGKTTTTFLIKHICEAAGLRCGLIGTVRYEIGERVLPALRTTPESLDLAELLAQIRDAGCRAAAMEVSSHALAQDRTRGLEWNVAVFTNLTQDHLDFHGTMASYFEAKAKLFAQLGAQQKKRTPVAIVNIDDRYGEQLLNKIDKNVRIITYGRSIRADFRASNPRVEFSGTSYQLDARGKSYLVRVPLIGTFNVSNSAAALATGVALGIPMPVLLQALTEFKGAPGRMERIWSHDRHRCAVVDYAHTPDALQKALEALRTVTPAGAKLAVVFGCGGNRDRQKRPEMGRIAAELADRVILTSDNPRDENPEAILDEIEAGMAGRAHLRIADRAEAIRRAVGQLGEGDILLVAGKGHETYQEIKGVKYHFDDRLVARQMLEEIRK"
  },
  {
    "reference": "MYLVTAEQMRRLDGETIQRLGIPAIALMENAGRVIAEEIVALCRRRGGEGCRNAAGWEDAGKDAFQVSADHALTLDNAAAERWFILVGKGNNGGDGLAAARYLRETGIAVTLVYAVPPESLSGEAALQRDAAAAMGLPAVVYGRDRLDLAECSGILDALLGTGAAGAPRGAYAELIAAANSSGKVIVSADIPSGLNADTGETHEPCIHASVTVCLAFLKRGLLQYPGAGAAGHVKVRAIGIPSSLAPESGVQVHLLTPEVLHAHLGVDLARRRSPEGHKGTYGHVLLAAGTLRMSGAGLLSARAALRAGCGLVTWALPQKLLPCIIGSAPELMLADVGGNEDGTWQAGSVSELLRLSKERDVLAIGPGLGRFEADTEWLRNLWEETDCPLVIDADALNILAEADYKSWASRRHPVILTPHPGEMARLASITTAEVQRDRISLAMGYAKEHGVTLVLKGAHTVIATPEGQAYVNTTGHPGMGTGGAGDVLTGIISSLLAQGLNEAQAAAFGVYLHGLAGERAARQRNNPAAIIAGDIIEAL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration2. The protein must bind to Mg(2+) in order to perform its enzymatic function.3. The designed protein must possess ADP-dependent NAD(P)H-hydrate dehydratase activity, kinase activity, metal ion binding, ATP binding to support nicotinamide nucleotide metabolic process under certain conditions.4. The protein should be able to bind (6S)-NADPHX, K(+), AMP ligand in a variety of conditions5. The protein should have a low Km value for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.6. The designed protein must have at least one YjeF N-terminal domain. The designed protein sequence is ",
    "response#1": "MQEIDRLAEANFNLPVRMLMENAGAAVAEAVQVFCHTQRARGSRSRYAQSLMRICLLVGAGNNGGDALVAARHLQSDGFKPVVVLCVSSERLAALKSACAENLTVLHKMSIPVLFASDGFADTAAEKAQIQHELALCDVIVDGLLGTGVKGKVRDPMASVIRMANSAGKPIIAIDLPSGIDSDTGQVLGVCIKATATVALGTCKPCHFLHPGAEFRGQLSVHTIGIPRQLIEPEEFSGLPLVRAVDAHKGSFGHVLLIGGSVGKSGALALAGRAALFAGAGLVTLASPRQLLPAIAASQAEYMTFPLAETAAGTISYENLHAPAFGFMLDDKSVLGVGPGVDVHEDTQKFVRELVQRTERPVVLDADGLNAFLFPDKLLRERKTKFLALTPHPGEMARLLGTTSAKVQEDRLNVALKSASRWDAHVVLKGFHTIVAAPDGSVFVNTTGNAGLAKGGSGDVLTGILAALTAQLGTEDWLRVLVLGVYLHGLAAELATKGIDLSGLLAGDVAVAVPQARLKLLTSEFVNSLMPPVKRNWHKYERGYVTGLAGSPDMPGAAILASWAALTSGAGIVRLIHQEGMNAELSAAPLELVRQTYSPQDLEPVIEVMNKSSASFIGPGMGRTPDAFLVLKTLFKYLSKPCVIDADALTMLSEKEVPLPPSAVMTPHHGEMLRLLGKDKXYFSTEQLLQLAKNFAXRKKITLVLKGAPTFILQKGHPIYVNPRGSPGMATAGSGDVLTGMIAAFXAQHLQPIQAAIVAVYLHAVAGEYAALXKTEHCLIASDIIQNFPKAFREIK",
    "response#2": "MRIEDVYIWDINAKWLGITPYQLMENAGAGVAKIIEERVGEGLKIAVFSGTGNNGGDGFVAARHLSFENNVTLFLVGDEAKIGSEEARHNWEILRKLDFVKIIVLKDSTYIKPLDLSGFDVIVDALLGAGTRGEPREPIRSAIEKINKYAGRVKIVSVDLPSGYPSDVRVKCDFAVTFQWDKEEYGGFERVISKIGYPPRTLVGPGDAKFALQKKGEHKGQNGKLLIIGGSEEYFGAPYLAAKAASYIVDLVYLAMPEYSARRINDPNIILRPFEGKNFTKEDVEDVLSIADGVDAVVLGPGIGEKAETKDFVIEFVRWCEKPMVIDADALKAIAEDLDVLKGKEFVLTPHPGEFKILFGKKPEGSLEEKAELVMKKAQDINGTILLKGRHDIISNGIAWKYNKTGNNGMTTGGTGDVLSGLVGALLALENKPLRAASVGAFLNGLAGDMVKEEMGENFTALDVAEKVSYAVKWVDTLDQRCYTQYALTEDILMEHAAIGLLNHIIKSNRKTQQVLIVCGPGNNGGDGLALARLLFEYASVQLYLPFGAKSVMAQRQLERLHQLNIKLINELPRTSPDIVVDCLFGSGLNKPLNNSAVELIDHLNRFDSLKIACDIPSGLNSQGQPSPIAFYADKTATMGGLKTSLFSDDAKDYIGELTVITLGLPTTSYSTQEIRAMLPVRQPAGHKGSFGRVLIVAGSQGMTGAAILCARGALRAGAGLVSVALPETIAPVIQAAVPEVITVPLPDCDGRLTDPDILDHLHDPLAQADVLAIGPGLSRDLKTAELVRRIIGGFAGYQVIDADAIHAIADRPELLASISDRAVLTPHPGEFAALIGSTAREVDTNRLDAIASFLSLYSLTVVLKGRPTVIGLPKGPLVVNPTGNTGLATGGSGDVLTGLMAGLLAGGSSIADAAIAGPFIHGLAADHWAASFSERSLTPSDLIEMLPRLLKELE",
    "response#3": "MREIDNHTIEVLGIPGIVLMENAARGVCEVIYDRVKGSSALVVCGKGNNGGDGLAVARNLYNMGYDVEVILTAPVEELKGDAKKNAEILSALPVPVHVVDSTEKLFELYTFAKECDFIVDGLFGTGLSKPLSGFYAELVDFINRLNKTVISIDIPSGLSADTGQVIGPHVKADYTVTFAFPKVAHVMPPACYSVGEVFVVDISIPEDVPHKYERGYVTGLAGSPDMPGAAILASWAALTSGAGIVRLIHQEGMNAELSAAPLELVRQTYSPQDLEPVIEVMNKSSASFIGPGMGRTPDAFLVLKTLFKYLSKPCVIDADALTMLSEKEVPLPPSAVMTPHHGEMLRLLGKDKXYFSTEQLLQLAKNFAXRKKITLVLKGAPTFILQKGHPIYVNPRGSPGMATAGSGDVLTGMIAAFXAQHLQPIQAAIVAVYLHAVAGEYAALXKTEHCLIASDIRS"
  },
  {
    "reference": "MFGGAKPSFGATPAATSFGGFSGTTTTTPFGQSAFGKPAAPAFGNTSTFAAQPAQQSLFGAAATPAQPAGGLFGANTSTGFGSTATAQPTAFGAFSQPQQTSNIFGSTQTAASTSLFGQSTLPAFGAAKPTMTAFGQTAAAQPTGSLFGQPAAATSTTGFGGFGTSAPTTTNVFGSGTASAFAQPQATAVGASGVNTGTAVAKYQPTIGTDTLMKSGQANSVNTKQHCITAMKEFEGKSLEELRLEDYMCGRKGPQAGNAPGAFGFGAQVTQPAQPASGGLFGSTAQPSTGLFGQTVTENKSMFGTTAFGQQPATNNAFGAATQQNNFLQKPFGATTTTPFAAPAADASNPFGAKPAFGQGGSLFGQAPATSAAPAFGQTNTGFGGFGTTAGATQQSTLFGATPAADPNKSAFGLGTAASAATTGFGFGAPATSTAGGGLFGNKPATSFAAPTFGATSTASTPFSNFGLNTSTAATGGGLFNSGLNKPATSGFGGFGATSAAPLNFNAGNTGGSLFGNTAKPGGGLFGGGTTTLGGTGAAPTGGLFGGGTTSFGGVGGSLGGGGFGMGTNNSLTGGIMGAQPTLGIMTPSHQPIHQQILARVTSPYGDSPIFKDLKLSSEADATRATNPAAQQAVLDLTSNQYKISTSNNPAPMKVKALGSTLNRKSLFDGLEEFDASVEGFNLKPSAKRLVIKPKVKSVEGGNPSSSIGSAPNTPQSRPKGATPNKERESFSGAIPSEPLPPAGNSPGATNGRESQDNGRRESWLHP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should emphasize Polar residues compositional bias.2. The ideal protein would be one that plays a critical role in larval lymph gland hemopoiesis, RNA export from nucleus, positive regulation of defense response to virus by host through serine hydrolase activity, chromatin DNA binding, promoter-enhancer loop anchoring activity within nuclear pore, nuclear pore central transport channel, nucleoplasm.3. The designed protein must have a Nucleophile active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "LMSYLQLFLNAITTVVIFSFILSFIIALQKDLKSTWEQRKHELQYESRICQEQYLTNRCNQTPGLPALGEQCAIWKQCMDRNNDIFFRARSTLSAKLFGDIINSFIDPLNWKTLFVIFCGVITWCFSSNFLLGFVF",
    "response#2": "LMSYLQLFLNAITTVVIFSFILSFIIALQKDLKSTWEQRKHELQYESRICQEQYLTNRCNQTPGLPALGEQCAIWKQCMDRNNDIFFRARSTLSAKLFGDIINSFIDPLNWKTLFVIFCGVITWCFSSNFLLGFVF",
    "response#3": "LMSYLQLFLNAITTVVIFSFILSFIIALQKDLKSTWEQRKHELQYESRICQEQYLTNRCNQTPGLPALGEQCAIWKQCMDRNNDIFFRARSTLSAKLFGDIINSFIDPLNWKTLFVIFCGVITWCFSSNFLLGFVF"
  },
  {
    "reference": "MAIRLYTFSANSRLVHSSSNGCICCSYLTFLPPSWYISSFTSNKMHRRVSLLVYDTCRWILPSTCHSVGFHSTTMKEAISAKKFVFTKRFQGLPQLDNFSLEKEELPELKPGDVLCEALYLSVDPYMRPYSLSWPLGTTMKGGQVSKVIESKNEKFPVGSLLVGYFGWRTHTVVNPDVRSKDFMEQITVLPNLGTLCPSVGLGAVGMPGNSAYFGFLEICKPKHGDVVVVSGAAGAVGSLVGQIAKIKGCHVIGFAGSDEKVKWLTDELGFDNAYNYKTCDWKSSLKEGAPNGVDCYFDNVGGQLSVAVRSHMNDFGRISVCGAISLYNDTVPTLVPCVEPAMVFKQLRMEGFLVHRWTHRFLEGMGQMSTWIQEGKIKIKETHTNGFENMPAAFIDMLKGGNFGKAIVKA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein contains novel Enoyl reductase (ER) domains that confer a unique function or activity.2. The protein should have a fast turnover rate for leukotriene B4 + NADP(+) = 12-oxo-leukotriene B4 + H(+) +   NADPH.3. The protein should have 13-prostaglandin reductase activity, 15-oxoprostaglandin 13-oxidase activity, 2-alkenal reductase [NAD(P)+] activity and be active in cytoplasm. The designed protein sequence is ",
    "response#1": "GLTSHFSPVTRVIESKYSDFPVGTVVMHRYGWCTHAVLNAKEQDPQSPIRKAPADYPKDLPLSLLLGAVGMPGMTAYFGLLKLCDPKEGETVFVNGAAGAVGNLVGQIAKIKGCKAVGAAGSDAKVAWLKEIGFDAAFNYKTVSSLDAALKEAAPNGIDCYFDNVGADFTSTVLNHMNQFGRVAICGSISTYNDKDPAGRGPYPFVTILSKQLTVTGFIVLRWFPEWPQGMAQMVQWIKEGKIKHREHVTEGFENMPKAFMGMLVGENTGKAVL",
    "response#2": "TEENVRIVEEELPELKNGEYLVEAVYLSVDPYMRAYAPNYKIGDTLIGTQVAKVIQSKSSKFPVGQYVVGGFGWRTHSIIKDDIPKTEWNVPYIVDIDEKFPLSLALGVLGMPGNTAYLGLLEICKPQPGETVVVSGAAGAVGSTVGQIAKIKGCKVIGIAGSDEKAKWLKSIGFDHIINYKTDDLQKALEEAAPNRVDCYFDNVGGETSSIVLQNMNKYGRIAVCGAISGYLSKDMTDYPKATSVQRPMISFELTMRGFLVNQFLDKWFDAIAQNQQWLREGKLQYRETLYEGFDNMLKALVGVLKGENC",
    "response#3": "TDSNFQLKTTELPPLKNGEVLLEALFLSVDPYMRVAAKRLKEGDMMMGEQVARVVESKNSAFPTGTIVLGFLGWTTHSISDGKKLQKLLAEWPDTVPLSLALGTVGMPGLTAYFGLLEICGAKSGDTVMVSAAAGAVGSVVGQIAKLKGCKVVGSAGSDEKIAYLKKIGFDVAFNYKTEKSLEQALKNASPDGYDCYFDNVGGEFSNAVISQMKTFGRIAICGAISTYNSTHALPPGPPPEKIIYQQLRMEGFLYSRWPEDVRQKALEDLLKWVSEGKIQYHEYIIEGFENMPAAFMGMMKGDNLGKTIL"
  },
  {
    "reference": "MKVVVGITGGIAAYKAVGVVRALVLAGHDVHVVPTQAALRFVGRPTLEAISRNPVESDLYEGVAEVRHVALGQTADLIVIAPATANTLAKLAIGLADDLLGTTVLASGAPVVVAPAMHTEMWAHAATQANVRTLEERGVLVVGPASGQLTGSDSGAGRMSEPADIVQAALDAALAANPDLDRTVVPEVTDQLAGRKVLISAGGTREPLDPVRFLGNASTGSQGVALAEAARDRGAEVVLIAANLEVPAPIGVRVVPAPTAQAMLDAASVEAADADLVIMAAAVADYRPATTAETKLKKDEIGDVLTVELVRNPDVLASIAANRPRPGQVVVGFAAETESDAGRLLALGREKIARKGADFLVLNEVGFDRGFGPGATAVTVIDRSGAVVGEARGAKSSVAAAILDLVAPS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should contain one or more Flavoprotein domains that are essential for its function.2. The desired protein should be able to perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO23. The designed protein must be optimized for FMN binding. The designed protein sequence is ",
    "response#1": "LLGVTGGISAYKSVELARLLLKAGASVRVVMTEAAGRFVSPLTFQAVTGNPVRTALFDEKHEAAMGHIELARWADRMLIAPASADFMARMAQGVANDLLSTVYLACPSPVAIAPAMNTNMWQHPAVQHNLSVLEGRGVRVLGPASGEQACGDKGFGRMLEPMEILATVAALIA",
    "response#2": "MKVALGVTGGIAAYKAAEIVRLLQDRGIRVQVVMTRAAQEFVRPLTFAALSGEKVITGMFSSGDEHQPNIDSAIEHIAVAQSIDALVVVPATADVLAHFAQGIATDFLTTLYLATTAPVVVAPAMNVNMWNHAATQANLEILRQRGVRVVEPDAGYLACGMTGPGRLADNEAIVTAVLHAVALAGAGGA",
    "response#3": "MKVALGVTGGIAAYKAAEIVRLLQDRGIRVQVVMTRAAQEFVRPLTFAALSGEKVITGMFSSGDEHQPNIDSAIEHIAVAQSIDALVVVPATADVLAHFAQGIATDFLTTLYLATTAPVVVAPAMNVNMWNHAATQANLEILRQRGVRVVEPDAGYLACGMTGPGRLADNEAIVTAVLHAIVAQR"
  },
  {
    "reference": "MRPSAAAGGGGGGGGRRKAAAAAAAASREWLVVPASGQARVEEAGKHAVMARTGLPARDLRVLDPLLSYPSTILGRERAIVVNLERVKAVITAAEVLLPNSKDPAFASFVCDLQARVLASSSDQAAEFTDMEGESSAVTSPFPALTSTTPNELEMTNKNSNVVGGMTHSNSMPTLTAAKDGNTKVLPFEFRALEVCLESACRSLEEETSTLEQEAYPALDELTSKISTLNLERVRQIKSRLVAISGRVQKVRDELEHLLDDEMDMAEMYLTEKLTRQEISETSSRVEVDDPSQLEVDRDEDYRSEADVSNGTFIGYKPHIEELEMLLEAYFVQIDGTLNKLSHLREYVDDTEDYINIMLDDKQNQLLQMGVMLSTATVVITAGVAVVGLFGMNIGISLYADPTNEEEKRASNMKFWETTLGTIAGCTVMYIVAMGWGKRSGLLQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should contain at least one Required for magnesium transport activity motif to achieve its intended function.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. The protein must exhibit the following characteristics: Magnesium transporter that may mediate the influx of magnesium..4. A protein with magnesium ion transmembrane transporter activity functions is required to enable magnesium ion transport.5. The protein must contain a signal peptide for proper functionality. The designed protein sequence is ",
    "response#1": "LQPYDMAFIFKEMPEKHRARYLSYLTVDDITDMIGELEREFQLVVLNKVGKTKATLAMNKMDNDDLAQLLEEMDEELKEQLLSSMEASESKAVQLLMNYPADSR",
    "response#2": "MPMVSGMTGNTGTQSLAVVIRGLSKEEMNKKTIVRLIFREFRTSIFIGAVCSVLIAIVSIIWQGNALLGFVVASSLFLTLIIGTMSGTIIPIILHKLKVDPAIASGPLITTLNDILSLLIYFGC",
    "response#3": "MPMVSGMTGNTGTQSLAVVIRGLSKEEMNKKTIVRLIFREFRTSIFIGAVCSVLIAIVSIIWQGNALLGFVVASSLFLTLIIGTMSGTIIPIILHKLKVDPAIASGPLITTLNDILSLLIYFGA"
  },
  {
    "reference": "AVRHKAPVRGALRFARPICRKMLLSREMLMGIGAVVLPLLLSVCSRARSSSVPRRMRIFGHRGASAQAPENTEASLRLALGGMRADGAEFDLQLTKDGQLVLLHDDTLLRCSSASKPPSITSTEYDRLRQTDIGEMTLQEARQVDVGSWYHEDFGAERVLTFRRALQILCDEFPDKCFFAEVKGGDTASVPRIAEEVASSDATPQQVIFIGFDIDVMKLCKESLPSFRSFLITAAGHGENDVDRARALLAEQIDAAAAAGLDGIDVHADRTLVDEAVVASAKRQGLEVAVWVWKKLPESDTAATWEHMERVGVDYFTSDLPPEIGSRLSRKLVAGAPEGTRKTITFVTGNAKKLEEVVHILGSEGELPFDLTNRKVDLPELQGTPEECAVEKCKLAAEEVQGPVMVEDTSLCFNALSALPGIYIKWFLKGLGHDGLNRMLAGFDDKSAYAQCIFAYTDGPGADVEVFVGRTPGRIVPARGPLDFGWDPIFQPDEGEGMTYAEMDKSSKNLISHRGRSLRKLRDYLTRQTT",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must be able to nucleoside triphosphate diphosphatase activity.2. For general function, the protein need meet that Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives; The enzyme does not distinguish between the deoxy- and ribose forms The designed protein sequence is ",
    "response#1": "MPIGVPKVPFRNPGEDFDKA",
    "response#2": "MGRKK",
    "response#3": "MKILGIDTSCDDTSVAILDGNRVLSNVVSSQVELHKKYGGVVPLLSQRHHQENITPVLEEALEKANLKMSDLDAVAVTVGPGLIGALLVGVAFAKGLSILWKKPIVG"
  },
  {
    "reference": "MALNIAVVGATGAVGKEMLTILAERLFPAGEVHALASRRSVGTELSYGDKTLKCKDLETFDFSSVDLVLMSAGGDVSKEWSPKIAKAGAIVVDNSSAWRMDPDVPLIVPEVNADALDDGIRKGIIANPNCSTIQTVVALKPLHDAAVINRVSVATYQSTSGAGAEGMDELWKQTRAIYVNDPIEPQAFIKQIAFNVIPKIDVFMEDGSTKEEWKMVVETKKILDPKIKLFATCARVPVFVGHCVATHIEFENEISAKKARSILREAPGIMLIDRADEEDPAFVTPVESVGEFATFVSRVREDPTVENGLALWIVSDNLRKGAALNAVQIAELLLNKGIVKA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a binding site that is specific to the target substrate, NADP(+) ligand.2. The design of the protein must focus on the precise arrangement of amino acids in the Acyl-thioester intermediate to enable effective catalysis of L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.3. Ideal protein should have a binding affinity for key enzymes in the L-threonine from L-aspartate: step 2/5, L-threonine biosynthesis, Amino-acid biosynthesis pathway to enhance the overall efficiency of the pathway.4. The protein design must be able to perform NADP binding, NAD binding, aspartate-semialdehyde dehydrogenase activity, protein dimerization activity in order to contribute to lysine biosynthetic process via diaminopimelate, 'de novo' L-methionine biosynthetic process, threonine biosynthetic process, diaminopimelate biosynthetic process, isoleucine biosynthetic process.5. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..6. The designed protein should contain one or more Semialdehyde dehydrogenase NAD-binding domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MATVAIIGATGAVGREMISILEQRAFPMKALRLFASPRSVGTRIPFGGTSIAVEALEHGMPEGIDLVLFSAGKGISKEWAPKFREAGALVIDNSSAFRADAECPLVVPEVNPQALSNVKRIVAGTCVRVPVFTGHLEPLRQRYGLRRVIVSTYQAVSGTGREAMETLRHEAAQTLAQEPVTPSTYPTPIAFNVLPYCDKFGDLDYTGEEWKLTRETQKIFRLELPVSATAVRVPVAVSHAEAVYVELERPFEVDEVRTLLREAPGIRVVDDPEHGIVPTPLEAAGQDLVFVGRIRRDPFIAQGLHLFVVADNLRKGAALNSYEVLLEALLEPINKLSPIRKVHVSSYQSASGAGAVAMAELQQQYKEIVETGEVKTIKKFPHQLAYNVIPQIDSMTETDYTKEEMKMFNETRKIMHSDVRTSATCVRVSSLRSHSESVWFETEQPLSVARIRQALEAAPGVKLVDDPQNYVYPMPLESAGHDDVYVGRVRKDLADDNGNTLWLTGDQIRLEPINKLSPIRKVHVSSYQSASGAGAVAMAELQQQYKEIVETGEVKTIKKFPHQLAYNVIPQIDSMTETDYTKEEMKMFNETRKIMHSDVRTSATCVRVSSLRSHSESVWFETEQPLSVARIRQALEAAPGVKLVDDPQNYVYPMPLESAGHDDVYVGRVRKDLADDNGNTLWLTGDQIRD",
    "response#2": "MTRIGIVGATGQVGAVMRRILAERSFPVAEMRYFASARSAGTSLPWEGVDVRVEDAATADPSGLDIALFSAGGATSRELAPRFAAAGAVVIDNSSAWRMDPDVPLIVAEVNAAEIANCTTNCVLKPLHDAATIKRVVVSTYQSVSGSGKEGMDELFEQTRAVFVADPISAKKFTKRIAFNVIPHIDVFMEDGYTKEEWKVLAETKKMLDPKIKVTATAVRVPVFIGHAESVNLEFEKPITADEARDILREAPGCQVIDKREDGGYITPYESAGEDATYISRIREDATVENGLALWVVSDNLRYDNEYGYSRRVLDLIVHLA",
    "response#3": "MTKIAVVGATGRVGHEVLSILAEFQDEGKISIDSVITFASKKSKGKKVSFGNEELTVLCLENYDFADIDIAIFCAGYHVSEKYVPIATEAGCIVIDNSSYFRMKEGVPLIIPEINKEKIDGFDLPKNIVANPNCSTIQMVVALKPLHDKFKVKRVVVSTYQSVTGAGKRGIDQLFAEIKGDKILNPKFPHQIAFNCIPHIDNFYEDGYSKEEHKIMKETIKIIGDNSIKITATCVRVPVIGGHSEAVNIEFEKDFEIEEIRDVLNNSPGIVLYDDPYNNAYPMPLISHEKDEVFVGRIRRDYSSENALNLWIVADNLRKGAALNAVQIAEKLVS"
  },
  {
    "reference": "MTAEMDMALMQGCVTFQDVAICFSHEEWRLLDETQRLLYLSVMLQNFALINSQGCGHKTEDEERRVSTRASKGLRSETTPKTNLCEKCVPILQDILCLPGLPGQKHSTEASSKVDQHQDHNSTGKPLEKNADRSSYLFYLSAKSFPSWDVEKDLPDILSLLKSQVCPKTKKYRKSTEGRKETSHESDKSEECQSLSSQKQTLAHHPKTSNGKKLYECSKCGKTFRGKYSLDQHQRVHTGERPWECRDCGKFFSQTSHLNDHRRIHTGERPYECSECGKLFRQNSSLVDHQKTHTGARPYECSQCGKSFSQKATLVKHKRVHTGERPYKCSECGNSFSQSAILNQHRRIHTGVKPYECRECGKSFSQKATLIKHQRVHTGERPYKCSECGKSFSQSSILIQHRRIHTGARPYECSQCGKSFSQKSGLIQHQVVHTGERPYECDTCGNSFSQCSSLIHHQKCHNA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that can perform DNA-binding transcription factor activity, RNA polymerase II-specific, metal ion binding, RNA polymerase II cis-regulatory region sequence-specific DNA binding in nucleus to support regulation of transcription by RNA polymerase II.2. The protein must exhibit the following characteristics: May be a transcriptional repressor..3. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.4. The designed protein must have at least one KRAB domain. The designed protein sequence is ",
    "response#1": "MRDEVAEKEKADINVTLVFQGYENTPIMVCVDGIVFSKPDLVTCLEQRKKPWSMKHPGLTQHNC",
    "response#2": "LSFKDVAVVFTEEELELLDSTQRQLYQDVMQENFRNLLSVGERNPLGDKNGKDTEYIQDEELRFFSHKELSSCKS",
    "response#3": "LTFRDVAIEFSLKEWQCLDTAQRNLYRNVMLENYRNLVFLGITVSKPDLITCLEQGKEAWSMKRHEIMVAKL"
  },
  {
    "reference": "MMTLVLWVSTLLSSVCLVAANIFEYQVDAQPLRPCELQREKAFLKQDEYVPQCSEDGSFQTVQCQNDGQSCWCVDSDGTEVPGSRQLGRPTACLSFCQLHKQRILLSSYINSTDALYLPQCQDSGNYAPVQCDLQQVQCWCVDTEGMEVYGTRQQGRPTRCPRSCEIRSRRLLHGVGDKSPPQCDADGEFMPVQCKFVNTTDMMIFDLIHNYNRFPDAFVTFSAFRNRFPEVSGYCYCADSQGRELAETGLELLLDEIYDTIFAGLDQASTFTQSTMYRILQRRFLAIQLVISGRFRCPTKCEVEQFTATSFGHPYIPSCHRDGHYQTVQCQMERMCWCVDAQGIEIPGTRQQGQPLFCAKDQSCASERQQALSRLYFETPGYFSPQDLLSSEDRLVPVSGARLDISCPPRIKELFVDSGLLRSIAVERYQQLSESRSLLREAIRAIFPSRELAGLALQFTTNPKRLQQNLFGGTFLVNAAQLNLSGALGTRSTFNFSQFFQQFGLPGFLVRDRATDLAKLLPVSLDSSPTPVPLRVPEKRVAMNKSVVGTFGFKVNLQENQDALKFLVSLMELPEFLVFLQRAVSVPEDRARDLGDVMEMVFSAQACKQTSGRFFVPSCTAEGSYEDIQCYAGECWCVNSQGKEVEGSRVSGGHPRCPTKCEKQRAQMQNLAGAQPAGSSFFVPTCTSEGYFLPVQCFNSECYCVDAEGQVIPGTQSTIGEPKLCPSVCQLQAEQAFLGVVGVLLSNSSMVPPISSVYIPQCSTSGQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream ; The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling 2. The designed protein should contain one or more Thyroglobulin type-1 domains that are essential for its function.3. The ideal protein would be one that plays a critical role in thyroid hormone metabolic process, thyroid gland development, thyroid hormone generation, iodide transport through hormone activity, signaling receptor binding, identical protein binding within protein-containing complex, endoplasmic reticulum, extracellular space.4. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "PRSCEIRNRRLLHGVGDKSPPQCSAEGEFMPVQCKFVNTTDMMIFDLVHSYNRFPDAFVTFSSFQRRFPEVSGYCHCADSQGRETA",
    "response#2": "PRSCEIRNRRLLHGVGDKSPPQCSAEGEFMPVQCKFVNTTDMMIFDLVHSYNRFPDAFVTFSSFQRRFPEVSGYCHCADSQGRERSL",
    "response#3": "PRSCEIRNRRLLHGVGDKSPPQCSAEGEFMPVQCKFVNTTDMMIFDLVHSYNRFPDAFVTFSSFQRRFPEVSGYCHCADSQGREPL"
  },
  {
    "reference": "MRDVQSEKDHRNIPINMVGIKGLKYPIIVMDRTNKRQHTIGTFNLFVDLPKDFRGTHMSRFVEVLDRHNMKVTPKNMESILDDMREALKATVAHVTVDFPYFIRKNAPISGIGSYSSYNCGFISTKNKEFDFILKVEVPVLTVCPCSKEISDRGAHNQRAMVNVQVRMNSLVWIEEIIEMVEDAASAPIFTLLKREDEKFITEVSYDNPRFVEDVSREVVLRFMNDPRISWYRVEVSSQESIHNHEAYACIEKGKSL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have GTP cyclohydrolase I activity to facilitate 7,8-dihydroneopterin 3'-triphosphate biosynthetic process.2. The protein should have a low Km value for GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate +   H(+).3. A protein that can modulate the overall flux of metabolites through the Cofactor biosynthesis, 7,8-dihydroneopterin triphosphate from GTP: step 1/1, 7,8-dihydroneopterin triphosphate biosynthesis pathway, enhancing or suppressing its activity as needed.4. The protein must exhibit the following characteristics: Converts GTP to 7,8-dihydroneopterin triphosphate.. The designed protein sequence is ",
    "response#1": "IKQAVKQILVAIGEDPEREGLRRTPDRVANMLEELTNGREENVQYTLFEGSSNMVIVAGIRFSTLCEHHLLPMLGVTHVAYIPSDKVIGVSKIPRIVVKYSRMLQLQERLTRQIMNEVSSATGSGDVMVLTEAYHTCMMIRGVRSASPLVSMAYKGKFNDSSLRLEFLEYL",
    "response#2": "IKQAVKQILVAIGEDPEREGLRRTPDRVANMLEELTNGREENVQYTLFEGSSNMVIVAGIRFSTLCEHHLLPMLGVTHVAYIPSDKVIGVSKIPRIVVKYSRMLQLQERLTRQIMNEVSSATGSGDVMVLTEAYHTCMMIRGVRSASPLVSMAYKGKFNDSSLRLEFLEYL",
    "response#3": "IQQAVRMILEAVGDDPDREGVLDTPKRVAKMYAEVFEGMHIDEEQYFETVFSEDHEEMVLVKDIPFYSMCEHHLVPFFGKAHVAYVPRGGRVVGLSKLARAVDTVAKRPQLQERITATVADSIMRKLDPHGVVVVVEAEHMCMTMRGVKKPGSKTITSAVRGMFEKDAAARAEVISLL"
  },
  {
    "reference": "MPPQNEVIIVSNDMNKSTHLHESSTDDDFPSLDDSLGDATTPPHKQSSMILAVNEPAGVVPAPPSQPQTSSTVHARKLDESEVLIVSPSYADDDSAYNTASGSHSHDHSDHLMDTSHVSVVTVGDEGVKVKDSSNELVKRQPNGVGIVPEDVNIIVNRFKQEKRSPDSSLSENGSVRGRRGIEVLVGGSGGSDVDSIGTNTSQESRHETDHNNKQQYPAAALMPPPPPSLTNNHNHETIDEEEEVVIRPKARVPAVVKSANAQGLTKEEIELRNLRKKTRKRTRKFEIDGVQMTTTTSRVIYGDDENGRIYDDHDFRKQELRELKLLQKQEKKQQTELHLKEQQAKEQQDRRFEQERSSLEKTYEADMDMLARQHKQLVEKTEQTQENELRSSSKRIRSEQEQELKIFRENLKQEIRLLKQEVDLFPKDKRKDEFKQRRSAMELDHEEKERAFLDSLKERHELLLRRLSEKHRDHLATINRNFLQQKQNAMRTREALLWELEEKQLHERHQLSKRHVKELCFMQRHQMIIRHEKELDQVKRMLQRKEEDMVKKQTMEKRALPKRIRAERKARDLMFRESLRISTNLDPEIERDRLKKFQEQEKKRYMQEERRFEVKHQKQLEELRATRESAIKELEQLQNEKRRALVEHEHSKLSEIDERLKGELREWREQLVPRKQVCMQIPV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Include a signal peptide as an essential component of the protein.2. A protein that can perform protein serine/threonine kinase activity in mitotic spindle midzone to support cell morphogenesis, establishment of mitotic spindle orientation, positive regulation of peptidyl-threonine phosphorylation.3. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "PGLQRSKSDLSERFSRAAADLERFFNFCGLDPEEARGLGVAHLARASSDIVSLAGPSAGPCSSEGGCSRRSSATVEERSLDRVPYGVSVIERNARVIKWLYGLRQR",
    "response#2": "PGLQRSKSDLSERFSRAAADLERFFNFCGLDPEEARGLGVAHLARASSDIVSLAGPSAGPCSSEGGCSRRSSATVEERSLDRVPYGVSVIERNARVIKWLYGLRQA",
    "response#3": "PGLQRSKSDLSERFSRAAADLERFFNFCGLDPEEARGLGVAHLARASSDIVSLAGPSAGPCSSEGGCSRRSSATVEERSLDRVPYGVSVIERNARVIKWLYGLRQY"
  },
  {
    "reference": "MKGFGLYILILLTLLVAVTYVLTSDVSQPLTYSDVYRMVENGEVSAIVYDGSMLYLEAPDGHSMSYKLPYFNLFLDQLREELKEQMDAGTLTFDLKSQPWWMMFIPYLVLIVILGLIWYFMMNKQGGSGGNPMQFGKVRARLASDQKKKVMFDDVAGAEEEKAELQEIVEFLKNPRKFVEIGARIPKGVLLVGPPGTGKTLIAKAVAGEAGVPFLSISGSDFVELYVGVGASRVRDLFEQAKKNAPSIVFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFGANEGVIVIAATNRKDILDPALLRPGRFDRQVYVGNPDVVGREAILKVHARNKQFDPDVRFDSIAKTTAGFSGADLENLLNEAALLAARRNKKLISLEEIEESLLKVVMGVEKKTHVISEKDKRLTAYHEAGHAICFHVLPTQDPVHHVTIIPRSNGAGGFTMPLPTDDKAYRTRRYMEENIIVDLGGRVAEQLMLDDISTGAYGDIKQATAMARAMVINYGMSDKIGPIDYDGTGGEIFLGRDFSANKGFSDHKSAEIDDEIHRIISEGYKACEKLLSEHKEQLVRVAEYLLRNETIDGNTFRKLFDGEEVPDRIGGGATEEELKAAAPADAEPTPNDLPSEELPH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The active site active site of the designed protein must be optimized for substrate binding and specificity.3. A protein that contributes to proteolysis, protein catabolic process by functioning in plasma membrane with ATP hydrolysis activity, ATP-dependent peptidase activity, ATP binding, zinc ion binding.4. The designed protein have AAA+ ATPase domains with specific sequence motifs that are crucial for its activity.5. For general function, the protein need meet that Plays a role in the quality control of integral membrane proteins.; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins6. The protein requires Zn(2+) to activate a critical catalytic site.7. The protein should have a high affinity for ATP. The designed protein sequence is ",
    "response#1": "IPSGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFDQAKKNAPSIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFTGNEGVIVMAATNRSDVLDPALLRPGRFDRKILVGRPLKFEYITPKDANMEGLAKRTVGMSGADLANVLNEAALLTARQHGTVIDDAALSAAHYDFGVDAEDPNRMVMNEKEKVNTAYHEAGHAIVGRLMPEHDPVHKVTIIPRGRALGVTQFLPEEDKYSLSRRAIVSQICSLFGGRIAEEMTLGAEGITTGASNDIERATQLARNMVTKWGLSEKLGPLHYGEDESGQPGQGNPVSGKTSAEIDAEVRRIIDDCYSRAKKILEENRDILEAMKDALMEYETLDAEQVDRLQGLRP",
    "response#2": "MVMSDKERTNTAYHESGHAIIGRLVPSHDPVYKVSIIPRGRALGVTMFLPEEDRYSLTKEGLESQICSLYGGRLAEELINGADQVTTGASNDIERATKLARNMVTKWGLSEKLGPLMYEEEQPDYYQQGTKMSPETQRAVDAEVREICDRCYARAKQLLEENRDKLELMKDALLEYETIDSAQIDAEFGDMTDEL",
    "response#3": "IPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKSAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPGHEDLLGRKDPHAYGMGKLLVEKAGDFDVDLKSIAQSTPGFSGADLANLINEAALLASREGRTDIDAHNFEAAVAGRVGKVLGMDVDASLIARGTPGFSGADLANLVNEAALFAARENRRMVSMAEFEVTRIISEKEKKITAYHEGGHALAAWAMPDIEPVYKLTILPRGRTGGHALLVPEDDKELMTRSEMIGRLVFAMGGRTAEELVFHEPTTGASSDIEQATKIAKAMVKEYGMSARLGAVKYGHEQGDPFLGRSAGKEADYSLEVAHEIDEEVRKLIETAHTEAWEVLSTYRDVLDDLVYEVLEKETLHRKDLERATGLPLLRPPKK"
  },
  {
    "reference": "MPKKDALDHLSLGQHTDYPNEYDPKQLQPVPRSLNREPIGLKDKLPFSGVDLWTGYEISWLNANGLPQVAVGYFAVPAESPNLIESKSFKLYLNSFNDSRFDTWEYVQQLMETDLSACAGTPVKVKLKQLSELEGESVATFPGHCIDDQNISISHYDYQPSLLATEQHETEEALHSHLLKSNCLITNQPDWGSVYIHYQGPKLDRAALLAYLVSFRRHNEFHEQCVERIYQDLKALGMKKLTVYARYTRRGGLDINPFRSDFEPALQMQRMARQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm that is critical for queuosine biosynthetic process.2. The design of the protein must focus on the precise arrangement of amino acids in the Proton donor, Thioimide intermediate to enable effective catalysis of 7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-   deazaguanine + 3 H(+) + 2 NADPH.3. The designed protein should interact with enzymes and substrates within the tRNA modification pathway to enhance or regulate its activity.4. The protein should be able to bind substrate, NADPH ligand in a variety of conditions5. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).. The designed protein sequence is ",
    "response#1": "MPNFLRPISRKKHRKKIKLNCLNLPFKGKDIWTLYELSWLNKNGLPQIAIAKIEIDVNSANIIESKSFKIYINSFNQMKFNNNIDFINILTNDLTKCICGQISIKYLDQYDASLLFPIARATKRAEIGVTGALPFLGADMWTAFELSWLNLRGKPQVALARFTVPCESPNIIESKSFKLYLNSFNNTRFADVDAVKARLRADLSEAVAYHPEDPVAYPKVYDPSLLFPISRAVGRTQIGIGVVLPFVGEDRWHAYELSWLDARGKPCVATATFHVPCDSPYLIESKSLKLYLNSFSAEVFNRAEALRLRIAADLSACAGAAVAVDPPGIHRGEKLNVYPEHYDPALLQPIPRERSRETMVRGDLPFTGVDIWTAYELSWLDSSGKPHVAVGEFWVPADSSAIIESKSLKYYLNSLNQHRFATREQARQAIAGDLSEAAGGEVQVTRPYGYPKVYDPSLLFPISRAVGRTQIGIGVVLPFVGEDRWHAYELSWLDARGKPCVATATFHVPCDSPYLIESKSLKLYLNSFSAEVFNRAEALRLRIAADLSACAGAAVAVDPPGIRKGEKLNVYPKVYDPSLLFPISRAVGRTQIGIGVVLPFVGEDRWHAYELSWLDARGKPCVATATFHVPCDSPYLIESKSLKLYLNSFSAEVFNRAEALRLRIAADLSACAGAAVAVDPAGIRKGEKLNVAEGYPKVYDPSLLFPISRAVGRTQIGIGVVLPFVGEDRWHAYELSWLDARGKPCVATATFHVPCDSPYLIESKSLKLYLNSFSAEVFNRAEALRLRIAADLSACAGAAVAV",
    "response#2": "MSNFLRPISRKKHRKKIKLNCLNLPFKGKDIWTLYELSWLNKNGLPQIAIAKIEIDVNSANIIESKSFKIYINSFNQMKFNNNIDFINILTNDLTKCICGQISIKE",
    "response#3": "YLDQYDASLLFPIARATKRAEIGVTGALPFLGADMWTAFELSWLNLRGKPQVALARFTVPCESPNIIESKSFKLYLNSFNNTRFADVDAVKARLRADLSEAVETALKAEQAFDEYQSHYAPELLYPIPRQLKRSELGITDANLPFVGEDLWNAYELSWLNSKGKPVVAVGTFRVPANSPNLIESKSFKLYLNSFNQSSFDNIAAVSATMSRDLSAAAGSPIIVTYPKVYDPSLLFPISRAVGRTQIGIGVVLPFVGEDRWHAYELSWLDARGKPCVATATFHVPCDSPYLIESKSLKLYLNSFSAEVFNRAEALRLRIAADLSACAGAAVAVSAAASAAVYPKVYDPSLLFPISRAVGRTQIGIGVVLPFVGEDRWHAYELSWLDARGKPCVATATFHVPCDSPYLIESKSLKLYLNSFSAEVFNRAEALRLRIAADLSACAGAAVAVS"
  },
  {
    "reference": "MLSKEIKFGEEARRALERGVDTLANAVKVTLGPKGRNVVLDRKFGSPTITNDGVTIARDIDLKDPWENLGCQLVKEVAIKTNDVAGDGTTTATLLAQAMIKEGLKNVAAGANPMIMRRGIDQAVKAVVDEIKTLSKPVESRDSIAQVAAISADDPEIGQLIADAMEKVGRDGVITVEESQSVGTSLEVVEGMNFDRGYISPYMITNTEKMEAVLEDPYILISDKKISSIGEVLPVLEKVVQTGKPMLLIAEEVEGEALATLVVNKLRGTFNCVAVKAPAFGERRKAMLEDIAILTNGQVASEELGVKMENVSLDMLGKARQIVIKKDETIIVDGAGSEQAIRDRSANIKRQLEETESEYDREKLQERLAKLSGGVAVIQVGAATETELKERKHRIEDALAATKAAVEEGIVSGGGVTLINAIKAIEKIEAQGDELTGINLVKKAMEEPLRQIANNAGIEGSVVVEKVKEAETGIGYNALTGVYESMIAAGIIDPAKVTRSAVQNAASISAMVLTTEAVVSELPGEDEMKGMGAGGMGGMGGMGGMM",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The desired protein should be able to perform ATP + H2O + a folded polypeptide = ADP + phosphate + an   unfolded polypeptide.2. The protein must be able to isomerase activity, ATP binding, ATP-dependent protein folding chaperone. The designed protein sequence is ",
    "response#1": "MGQ",
    "response#2": "MIIIK",
    "response#3": "MIRPLHDRVIVKRLEEERKTASGIVL"
  },
  {
    "reference": "MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFEDVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLIILGVAFGIRFYTKQSSA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should contain one or more Cytochrome b5 heme-binding domains that are essential for its function.2. The protein can be expressed in membrane, intracellular membrane-bounded organelle and perform metal ion binding, heme binding without triggering undesirable cellular responses.3. For general function, the protein need meet that May play a key role in the modification by desaturation of fatty acids in the endoplasmic reticulum, which in the developing seed is utilized for membrane synthesis and in the developmentally regulated production of large amounts of storage lipids; Is involved in the reduction of cytochrome P-450 and may therefore be involved in flavonoid biosynthesis in the petals.; Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases4. The protein should have a high affinity for heme.5. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "WGNHEGSMLLWVLILAMFGAAAAAFGGALPERLRARVLAVQGTIGVAFLVFVLFTSNPFLRLEEAPFNGRDMNPLLQDPGLAFHPPFLYLGYVGLSMAFSFAVAALIEGRVDAAWARWVRPWTLAAWIFLTIGIALGSWWAYYELGWGGFWFWDPVENASLMPWLLAAALLHSAIVVEKREALKSWTILLAIMAFGFSLIGTFLVRLSNLYESFMFLSWSSSVIHIILEVRSQKDRGLGAITAPSTMLTHGFATSGLPKEMQQSAMLVPALQSHWLMMHVSMILLSYATLLCGSLSSIAFLIITFRRNRRILSLLSARDNSFIWPFPSRNNLYLHEQEKSDLQNTFYLSFTNHRKCKLTQQLDYWSYRVIGLGFLLLTIGILSGAVWANEAWGSRWSWDPKETWALITWIIFAIYLHTRVNKGWQDENPAIIASLGSFIVWICYLGVDLLGIGLL",
    "response#2": "LSNLYESFMFLSWSSSVIHIILEVRSQKDRGLGAITAPSTMLTHGFATSGLPKEMQQSAMLVPALQSHWLMMHVSMILLSYATLLCGSLSSIAFLIITFRRNRRILSLLSARDNSFIWPFPSRNNLYLHEQEKSDLQNTFYLSFTNHRKCKLTQQLDYWSYRVIGLGFLLLTIGILSGAVWANEAWGSRWSWDPKETWALITWIIFAIYLHTRVNKGWQDENPAIIASLGSFIVWICYLGVDLLGIGLVLFANPFAA",
    "response#3": "SNLYESLCFLAWACTLTQLLVERSWPSPIVAAAATPMGLGCIAFASFALPDQLQSAAPLVPALRSSWLVMHVSVIMVSYAALLVGSLLSLAVLLTDRGEALELRSSSIGSGGFRQSMRVGSDGVLQLQSIRLSTGEQLDSLSYRTITVGFLMLTVGIVSGAVWANEAWGSYWSWDPKETWALICWLVYAAYLHTRLSRGWQGRRPALVAVVGLVVIAVCYIGVNLLGIGLGVMVGGWASNSKYPLLSNLYESFMFLSWSSSVIHIILEVRSQKDRGLGAITAPSTMLTHGFATSGLPKEMQQSAMLVPALQSHWLMMHVSMILLSYATLLCGSLSSIAFLIITFRRNRRILSLLSARDNSFIWPFPSRNNLYLHEQEKSDLQNTFYLSFTNHRKCKLTQQLDYWSYRVIGLGFLLLTIGILSGAVWANEAWGSRWSWDPKETWALITWIIFAIYLHTRVNKGWQDENPAIIASLGSFIVWICYLGVDLLGIGLTAFNLSNLYESFMFLSWSSSVIHIILEVRSQKDRGLGAITAPSTMLTHGFATSGLPKEMQQSAMLVPALQSHWLMMHVSMILLSYATLLCGSLSSIAFLIITFRRNRRILSLLSARDNSFIWPFPSRNNLYLHEQEKSDLQNTFYLSFTNHRKCKLTQQLDYWSYRVIGLGFLLLTIGILSGAVWANEAWGSRWSWDPKETWALITWIIFAIYLHTRVNKGWQDENPAIIASLGSFIVWICYLGVDLLGIGLSM"
  },
  {
    "reference": "MMKDKEHVIVGISGGVDSAVAACILIENGYRVTGLHIKVLDHSDDSLHLEKSPLIISDRKEFRFPVFSLNLSGSFRRDVIDYFLQDYLSGRTPNPCMVCNKLIKWKGLLKGADLLQATLVATGHYARIDCGREKCRLLKGTDSQKDQSYFLWMLSSEELSKTLLPVGNLTKQEVRELARRFGVRAAEKKESQEICFVPDNDYREVVKSSRPGLAEKLTGGEIIDTEGKVIGHHAGYPFYTIGQRKGLGISSPEPLYVNRLDPEKNRIRVGGKAMLQCRKLIAGQMNWTGISPPETSTRAAARIRYRDTGEACTIVPVEKERFEVIFDKPKQSVTPGQAVVFYRDDEVIGGGIIVEAISE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A key design requirement for the protein is the optimization of the Cysteine persulfide intermediate to achieve high catalytic efficiency for AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in   tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-   cysteinyl-[protein].2. A protein that can perform sulfurtransferase activity, ATP binding, tRNA binding in cytoplasm to support tRNA modification. The designed protein sequence is ",
    "response#1": "MQPWLKDWNIVDESRLLGCDDVIVKIRYRKQENHCSVTITPEGLLHIRLHEPLSAIAEGQAAAFYKDGLLLGGGIL",
    "response#2": "MNPWLKDWNIVDESRLLGCDDVIVKIRYRKQENHCSVTITPEGLLHIRLHEPLSAIAEGQAAAFYKDGLLLGGGIV",
    "response#3": "WLKDWNIVDESRLLGCDDVIVKIRYRKQENHCSVTITPEGLLHIRLHEPLSAIAEGQAAAFYKDGLLLGGGIL"
  },
  {
    "reference": "MGQADISRPVNPDGVEEIGQPTADLGMVMDCVEAGDITPPTKRKSKFSGFGKIFKPWKWRKKKSDKFKETSEVLERKISMRKPREELVKRGLLLEDSEQGGEDPGKLSHATLKNGHTTLIGSTRSSSPVQVEEESERIASLRKPVPEEEPKKRLGSSGSQPHSEAEFVPESIPKQPLLPPKRHLSSSHEANEGQAKDATSSGSLARPSSSASTTAITTAPAATMAATNPAKTVHSSGPPSQAPRTLPAAPASTHTTATLSLTHTGPAKQPPIPPPKPTHRNSNPVIAELSQAINSGTLLSKPSPPLPPKRGIPSALVPTSESAAATTATKAPSDQREKSACSVGSEPLLTPSSSPLPAHIPPEPPQSPPFPAKTFQVVPEIEFPPSLDLPQEIPQQESQKREVPKRMLDHSFGEPQVPPRLPPVPLHIRIQQALTSPLPVTPPLEGSHRAHSLLFENSDNFSEDSSTLGRTRSLPITIEMLKVPDDEEEEEQSHIFAFDEDVASTSVIPKLPQCLQEEEEGKESDSDSEGPIQYRDEEDEDESHHSALANKVKRKDTLAMKLNHKPSEPEMNMNSWPRKSKEEWNEIRHQIGNTLIRRLSQRPTPEELEQRNILQPKNEADRQAEKREIKRRLTRKLSQRPTVAELLARKILRFNEYVEVTDAHDYDRRADKPWTKLTPADKAAIRKELNEFKSSEMEVHEDSKHFTRYHRP",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Prioritize Pro residues, Polar residues compositional bias within the protein sequence to achieve desired characteristics.2. For general function, the protein need meet that Regulator of protein phosphatase 1 (PP1) required for neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development; Also acts as a regulator of migration of enteric neural crest cells (ENCCs) by activating PP1, leading to dephosphorylation and subsequent activation of cofilin (COF1 or COF2) and repression of the integrin signaling through the RHO/ROCK pathway .; Acts as an activator of PP1 by interacting with PPP1CA and preventing phosphorylation of PPP1CA at 'Thr-320'; During neural tube closure, localizes to the ventral neural tube and activates PP1, leading to down-regulate cell proliferation within cranial neural tissue and the neural retina3. A protein that can perform protein phosphatase activator activity, actin binding, protein phosphatase 1 binding in cytoplasm, lamellipodium to support enteric nervous system development, neural crest cell migration, neural tube closure, actin cytoskeleton organization, negative regulation of integrin-mediated signaling pathway. The designed protein sequence is ",
    "response#1": "LTENEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVQGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPASKTRGLGRGSLTENEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVQGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPASKTRGLGRGSQSLFSKPYSRETEYLNRRLSQRPSAEELEQRNILQKSLLARQYHEVNRQFQQQKALLHLTENEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVQGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPA",
    "response#2": "LTENEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVQGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPAPQRGRKRKRSDPEKEEEFTRPLKRPKTLPNTQLNRRLSQRPSAEELEQRNILQS",
    "response#3": "LKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVQGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVRDGYLMFGLTENEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVQGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPASPRTKRMLSYNELDSIIGRLLEVQGSRPGKNVQLTENEIRGLCLKSREIFLSQPILLELEQKDLSIFETGIKVVDLLAPYRRGGKIGLFGGAGVGKTLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVQGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVGICIYPCKNNNPARRKTRDLLD"
  },
  {
    "reference": "MAREDLIEVEAKVKEILPNTKFIVELDNGHTVVAHVSGKIRIHNIRILPGDKVTVELSPYDLSRGRITYRKK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that localizes to cytoplasm and can perform rRNA binding, translation initiation factor activity, ribosome binding with high specificity and efficiency.2. The protein must exhibit the following characteristics: Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds; Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex.; One of the essential components for the initiation of protein synthesis; Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC).3. The presence of S1-like domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MAKEELLEMEGLVDEILPDSRYRITLDNGHKLIAYTAGRVKKNHIRILAGDKVSLEVSPYDLSKGRITFRHG",
    "response#2": "MKKQNLIDMEGVVTESLPNAMFRVCLDNGCEVLTHISGKIRRNYIRILPGDRVKVELTPYDLTKGRITYRLK",
    "response#3": "DMEGVVTESLPNAMFRVCLDNGCEVLTHISGKIRRNYIRILPGDRVKVELTPYDLTKGRITYK"
  },
  {
    "reference": "MSCSLEVKEKLKPVQKTNLMGMPIAKVENFFAEFGEERFRASQTLQWIHQKGILDFESMTNFSKSLRRKLTSIAEVRPPDIVNCEESTDGTRKWLIQLNGGNCVEAVFIPERGRGTLCVSSQIGCALDCSFCATGKQGFNRDLSASEIIGQVWLASKSLGKFDQIASKCITNIVMMGMGEPLMNFDNVVDAMNLMLDDHGYGLSKRRVTLSTAGVIPAIEKLGNVSDVSLAISLHAPNDELRNKLVPINRKYPLSGLVSSAKRYLDKMPDNRRKITIEYTLMDKVNDRSHHARELADLLKELPCKINLIPFNPFHGAQYKRVTDFAMYRFRDILQQHGYTVTIRTTRGDDIAAACGQLAGQVNDKTRRQERYKQKLGEEQPVKMLM",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must have a Proton acceptor, S-methylcysteine intermediate that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "SEWFFLDFINPSSANLIKARRLIFSARSPYQEIEILDTQAFGKCLMLDGRMQSAQADEFIYHEALVHPAMVTHPNPESVLIVGGGEGATLREVLRHRTVRRAVMVDIDRQVVEACRLFLPEWSDGAFEDPRTELVIDDARHYLTSTDEQFDVIIMDITEPLENGPSAPLVTYEMFQIVRSHLKPQGIVAHQSGSTGTSELHFFASMYKTLRAVFRYVAPYSIQITSFAMPWGIVIASV",
    "response#2": "SEWFFLDFINPSSANLIKARRLIFSARSPYQEIEILDTQAFGKCLMLDGRMQSAQADEFIYHEALVHPAMVTHPNPESVLIVGGGEGATLREVLRHRTVRRAVMVDIDRQVVEACRLFLPEWSDGAFEDPRTELVIDDARHYLTSTDEQFDVIIMDITEPLENGPSAPLVTYEMFQIVRSHLKPQGIVAHQSGSTGTSELHFFASMYKTLRAVFRYVAPYSIQITSFAMPWGIVIASV",
    "response#3": "SEWFFLDFINPSSANLIKARRLIFSARSPYQEIEILDTQAFGKCLMLDGRMQSAQADEFIYHEALVHPAMVTHPNPESVLIVGGGEGATLREVLRHRTVRRAVMVDIDRQVVEACRLFLPEWSDGAFEDPRTELVIDDARHYLTSTDEQFDVIIMDITEPLENGPSAPLVTYEMFQIVRSHLKPQGIVAHQSGSTGTSELHFFASMYKTLRAVFRYVAPYSIQITSFAMPWGIVIASQ"
  },
  {
    "reference": "MGKGNNMIPNGHFHKDWQRFVKTWFNQPARRHRRKQNRIKKAKAVAPRPAAGPLRPVVRCPTIRYHTKVRAGRGFTLREIRAAGLNPAFARTIGIAVDPRRRNKSVESLQVNVQRLKEYRARLILFPKGKKVLKGEANEEERKLATQLRGPLMPVQQPAPKSIARAITEEEKDFKAYQYLRGARSIAKLVGIRAKRLKDAAENPDDVTKAPTAVKRNKT",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: As part of the LSU, it is probably required for its formation and the maturation of rRNAs.; The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel; Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell; The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain; The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules.2. The protein design should emphasize Basic and acidic residues compositional bias.3. The designed protein should have structural constituent of ribosome within ribosome, ribonucleoprotein complex, cytosol to regulate translation. The designed protein sequence is ",
    "response#1": "MESGYTLPSNIISNTDVTRLINSAEVQAVVRPAGEKTQKKSHVLKKNPLKNKQVLLRLNPYAKAYAAEKVGSAKVEQPAAEGG",
    "response#2": "MSGYTLPSNIISNTDVTRLINSAEVQAVVRPAGEKTQKKSHVLKKNPLKNKQVLLRLNPYAKAYAAEKVGSAKVEQAK",
    "response#3": "LSGYTLPSNIISNTDVTRLINSAEVQAVVRPAGEKTQKKSHVLKKNPLKNKQVLLRLNPYAKAYAAEKVGSAKVEQA"
  },
  {
    "reference": "MLNLKVIYFGTPQFAATVLEDLLHHDVNVIGVVTRVDKPQKRSSQPIPSPVKTLALSKNIPLLQPEKASDPQFIEQLKAFEADVFIVVAYGAILRQVVLNIPKYGCYNLHAGLLPAYRGAAPIQRCIMDGVTQSGNTVIRMDAGMDTGDIAGVSYVPVGPDMTAGELAEALSAQGGEILIKTLQQISDGTISHTPQDSSKASIAPKLSKEEGFVLWNHPAEKVYAQIRGVTPAPGAWTLYSYQDKPAKRLVIRKASLASNKGIYGNPGDVIVSDQQELLIACAEGAICLKEIQPEGKGAMDSKTFLNGHSNHKLKLSFQNN",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a binding site that is specific to the target (6S)-5,6,7,8-tetrahydrofolate ligand. The designed protein sequence is ",
    "response#1": "SRDPELAGAISQELTRQRDEIELIASENIVSLAVLQAQGSVMTNKYAEGYPGRRYYGGCQYVDIAEQLAIDRAKQLFGAGYANVQANSGSQANQSVFLALLQPGDTFLAMDLSAGGHLTHGARPNMSGKWFKAVHYGVRQQDSQLDYDQMEELAREHKPKLIIAGGSAIPRQIDFARFRKMADEIGAYLMVDMAHFAGLVAGGQHPNPLEHAHVVTTTTHKTLRGPRGGMILTNDEAIAKKINSAIFPGLQGGPLMHVIAGKAVAFGEALRPEFADYAAHVVANAKVLAETLMKGGIDIVTGGTDTHLMLVDLRPKGVTGKDAEAALGRAHITCNKNGIPFDPQPPTVTSGVRLGTPAGTTRGFGEAEFREIGELIAETLDAPNDEANLARVRERVAAL",
    "response#2": "LQEVDAEVFNCISGELNRQNSGLQLIASENFVSKAVLQAQGSIFTNKYAEGYPGKRYYCGCHFADIVENLAIERLCRLFGCKFANVQPHSGSQANQGVFAALLKPGDTVIGMSLDCGGHLTHGSAPSISGKWFNAVQYQVDRDTGLIDMDEIEKLAVEHNPSLIIAGSSSYPRVIDFKRFREIADKVGAYLLADIAHYAGLIAAGEFPSPVEYAHVITSTTHKTLRGPRGAVIMTNYEDIHKKIQSSIFPGMQGGPLMHVIAAKAVAFAEALKPEFKDYAKQIIKNSKALGEVFKERGLDLVTGGTDSHMVVLDLRSKSVTGKDAVLALEKLGIICNKNAIPFDPEKPFVTSGLRFGSAAETSRGLQESEFREIGSML",
    "response#3": "LQEVDAEVFNCISGELNRQNSGLQLIASENFVSKAVLQAQGSIFTNKYAEGYPGKRYYCGCHFADIVENLAIERLCRLFGCKFANVQPHSGSQANQGVFAALLKPGDTVIGMSLDCGGHLTHGSAPSISGKWFNAVQYQVDRDTGLIDMDEIEKLAVEHNPSLIIAGSSSYPRVIDFKRFREIADKVGAYLLADIAHYAGLIAAGEFPSPVEYAHVITSTTHKTLRGPRGAVIMTNYEDIHKKIQSSIFPGMQGGPLMHVIAAKAVAFAEALKPEFKDYAKQIIKNSKALGEVFKERGLDLVTGGTDSHMVVLDLRSKSVTGKDAVLALEKLGIICNKNAIPFDPEKPFVTSGLRFGSAAETSRGLQESEFREIGSMH"
  },
  {
    "reference": "MSLKGRHLIDPNQLSLAELDNIITLGLEIYRNPNHYREICKGKILGTLFYEPSTRTRLSFESAMLRMGGTVLGFSDANTSSAKKGESIADTIRVLDDYADLLVMRHPREGAPLLASQYATVPIVNGGDGGHQHPTQTLTDLITIHHNKGKIENLKVAFCGDLLFGRTVHSLLKTLSRFPNMEFVFISPSELKIPKHLKDEVIKSNNVEIVETTFLEKHMSEIDILYMTRIQKERFFNEEEYVKLKDSYILDLQKLRNAKEDLLIMHPLPRVNEIHPEVDTDTRAKYFEQAKLGVYVRMALMALQLGVIESC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be optimized for activity in UMP biosynthesis via de novo pathway pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. A protein with amino acid binding, aspartate carbamoyltransferase activity is needed to enhance 'de novo' pyrimidine nucleobase biosynthetic process, amino acid metabolic process in a specific context. The designed protein sequence is ",
    "response#1": "RHFISLDGLPRELLTEILDTADSFLEVGARAVKKVPLLRGKTVCNVFFENSTRTRTTFELAAQRLSADVISLNVSTSSTSKGETLTDTLRNLEAMAADMFVVRHSDSGAAHFIAEHVSPNVAVINGGDGRHAHPTQGMLDMLTIEAIEEV",
    "response#2": "EGLKVAIAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMMFLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVEAPKSRIVQQMTNGVFVRMAILEI",
    "response#3": "EGLKVAIAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMMFLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVEAPKSRIVQQMTNGVFVRMAILES"
  },
  {
    "reference": "MQKLVLASNNAGKLKEFSELLAPFEFEVIPQGQFEIPAAEEPFDTFLENALAKAKHASQLSGLPALADDSGICVNVLGGKPGVRSARYAGDHASDTDNNHKLLQDLANELDRTAYYICALVMVNSADDQNPITVQARWDGEVTDIPRGDKGFGYDPYFYIPELGKTAAELSSAEKNAFSHRGQALRILIAELLKLNQRV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.2. A protein with ribonucleoside triphosphate phosphatase activity, metal ion binding is needed to enhance nucleotide metabolic process, purine nucleoside triphosphate catabolic process in a specific context.3. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "ERRS",
    "response#2": "MTLLSWTR",
    "response#3": "MPLH"
  },
  {
    "reference": "MKETMMETLSQRLNALQEKILEHYEQDSKLIYDQINYWKYVRLENAIFYAARERGMHTIDHQVVPPGTTSKAKAYQAIELQMALESLAQTDFNKEEWTLKDTSNEMWQTKPKQCFKKKGVTVEVWYDGNKDNSMHYVVWGAIYYKTHTDTWCKTEGYVDYWGIYYVHEQHKTYYEVFKQDAQMYGTSGKWEVHCNGNIIHCPDSMYSTSDDTVPTTELTAELQHTTPAHTAATTPCTKKTKSAPSCKCGVSRPSETDGVFVDLVTSKGCNKRRHQCCGDTTPIVHLKGDKNGLKCLRYRLRKFNSLYENISCTWHWIGGKGSKHTGILTVTYTTEAQRQKFLETVRIPPSVHVSVGYMTL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box.2. The protein must be able to DNA binding, DNA-binding transcription factor activity. The designed protein sequence is ",
    "response#1": "LEAKELWDQFHKIGTEMVITKSGRRMFPPFKVRVSGLDKKAKYILLMDIVAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMAKPVAFHKLKLTNNISDKHGFTILNSMHKYQPRFHIVRANDILKLPYSTFRTYVFPETDFIAVTAYQNDKITQLKIDHNPFAKGFRR",
    "response#2": "DPKVNLEAKELWDQFHKIGTEMVITKSGRRMFPPFKVRVSGLDKKAKYILLMDIVAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMAKPVAFHKLKLTNNISDKHGFTILNSMHKYQPRFHIVRANDILKLPYSTFRTYVFPETDFIAVTAYQNDKITQLKIDHNPFAKGFRDTGNGW",
    "response#3": "LEAKELWDQFHKIGTEMVITKSGRRMFPPFKVRVSGLDKKAKYILLMDIVAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMAKPVAFHKLKLTNNISDKHGFTILNSMHKYQPRFHIVRANDILKLPYSTFRTYVFPETDFIAVTAYQNDKITQLKIDHNPFAKGFRR"
  },
  {
    "reference": "MGSTGLCFYGHCIVMFLLQMVTASSEPFIVNGLEGPVLASLGGNLELSCQLSPPQQAQHMEIRWFRNLYTEPVHLYRDGKDMFGEIISKYVERTELLKDGIGEGKVTLRIFNVTVDDDGSYHCVFKDGDFYEEHITEVKITAINLQVQIHVHPPNTKGVIVECHSGGWFPRPLMQWRDRRGEVIPAASKSHSQGRDKLFNMKISLLISESFFQKVICCLQNPLTGQEERTSVILSDAFFSWNRIWKMILGIILSMMVVSIFVFSCLLHHEHKVCKWKWDAPWIKGLLIMTSSMVTVVLVMVYLHMKQRVPVSDVHFELDTLWVEDISVILCSLMVPATMLVSYTYFRLKDWCQHNHAQRVFTSN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Design the protein with a signal peptide incorporated.2. The Extracellular topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.3. The protein contains novel Ig-like V-type domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "DGEKIVIVALVHSTVLLPCENHDHMWTDRHREEDQQVVHWDRQAPGIPHDRADRLIDMYASGERRAYGSLFLRRKMNVSNSAFSQGDFTLFIPYLTRGDEGTYSCHLHHHYCGLHERRIFYLSVR",
    "response#2": "VHSTVLLPCENHDHMWTDRHREEDQQVVHWDRQAPGIPHDRADRLIDMYASGERRAYGSLFLRRKMNVSNSAFSQGDFTLFIPYLTRGDEGTYSCHLHHHYC",
    "response#3": "VHSTVLLPCENHDHMWTDRHREEDQQVVHWDRQAPGIPHDRADRLIDMYASGERRAYGSLFLRRKMNVSNSAFSQGDFTLFIPYLTRGDEGTYSCHLHHHYC"
  },
  {
    "reference": "MIMLIKVFVLLLVVYILHNSYKKYKKLDKNELKGPTPIPVLGNLHQLSSLPHRDLSKMTKDYGDIFRVWFADLYTVVISDPVLIRKIYVENHESFRDRPKIPSMKYGTYYHGTAASMGEDWVRNRGIVSSAMRKSNIKHIYEVINNQVDVLMSTMKKYEKRSEPFEPRYYMTKYTMAAMFKYIFNEDIGEDEDIHTGEIQKIMGPMNQVMEDFGTGSLFDVLEISQTFYLKWLELTEKNFPLLLKFFNGRYEQHLETIKPESPRDLLDILINEYGTNTHDDYLNIASTVLDFFFAGTDTSSTTLEYLFLMMANYPEIQDKVHQEVKSYLKQIGKDKVELNDRQSLPYVVAVIKETLRFKPVTPFGVPRSCVNEITIDEKYFIPKGAQVIINYPSIFENEKYFKNANQFDPSRFLQTTTTNTASNEESSFNSNLAFIPFSIGPRNCVGMQFAQDELFLAFANIVLNFTIKSVDGKKIDETISYGVTLKPKTRFKVLLEKRLI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. A protein that can perform heme binding, iron ion binding in intracellular membrane-bounded organelle, membrane, cytoplasm to support xenobiotic metabolic process, organic acid metabolic process.3. The protein must be engineered to have a high affinity for heme in order to increase its catalytic activity.4. The designed protein should be able to compete with endogenous proteins or inhibitors for heme binding. The designed protein sequence is ",
    "response#1": "MW",
    "response#2": "STTAF",
    "response#3": "MHHHHHHSSGRENL"
  },
  {
    "reference": "MTGTLERENWISGGKSLVLRKQHPGPLRPWRKRAAQLGGGCGWRTAVAPAKFCLWYVVPSWLWEPPGYLHSSLFLSILFQVTLLETALQSRPNLSLPLVRCGWACTQAMSTRSNCGSRSFLWAQTQADAASGLPRSRLGFLGLGGCGLIVKHGMTLRNWASFFVVFQAWSLMILQVLGDMLNIYYAYIQATLTLKVDVAPRLFFPEGGALKEHFSSMDSFQLREAGGTRIPRPALIYGRAVVTRTVTKAQSLKSALAWAALGCKHPVLSTLCEESQQGAWSEFRRF",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein localizes to the membrane.2. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "KGALLVCLAATMWGFDGIALTPRLFSLHVPFVVFILHLLPLILMSILFGKEEVKNIKKLQKNDLFFFFCVALFGGCLGTLCIVKALFLVNFKHLTVVTLLQKLQPIFAIILARLLLKEKLKRAYLFWGFLALLGGYLLF",
    "response#2": "KGALLVCLAATMWGFDGIALTPRLFSLHVPFVVFILHLLPLILMSILFGKEEVKNIKKLQKNDLFFFFCVALFGGCLGTLCIVKALFLVNFKHLTVVTLLQKLQPIFAIILARLLLKEKLKRAYLFWGFLALLGGYLLL",
    "response#3": "KGALLVCLAATMWGFDGIALTPRLFSLHVPFVVFILHLLPLILMSILFGKEEVKNIKKLQKNDLFFFFCVALFGGCLGTLCIVKALFLVNFKHLTVVTLLQKLQPIFAIILARLLLKEKLKRAYLFWGFLALLGGYLLL"
  },
  {
    "reference": "MNCRQFLSSHCSRDSSGGGGGSKMFSCNRSSFSGASGGGGRFSSSRSFGGGSSGACGRGGGGSFGSSYGGGSGGGFSAGSFGGHSRGFSGGSGGGFGGGFGGGFGGFSGGSGGGFGGPGGFGSGFDSGAGGILGADEKTTMQDLNSRLASYLDKVQALEDANKELESKIREWYDKQGSRTFHRDYSPYYDTIEDLKNQIVNITADNNKTLLDLDNTRMTLDDIRMKCDIENNLRLAVNADINGLRKVLDDLTMQKSDLEMHYESLQEELIALKKNHEDEMSQLTGQNSGDVNVEMNAAPGRDLTKILNDMREEYERISAKNRKDIEEQYETQMSQMEQEVMSSGQEMESNHKEVTQLRHSIQEMEIELQSQLSKKSALEKSLEDTKNHYCGQLQQLQEQIRSLEGQITEIRGEIECQNQEYSLLLNIKTRLEQEIKTYRSLLEGGQEDFESHESGQSHFGSGGSRQQGGIGGSHGRGSRGGSGGSYGGGSSSGGGSGGSHGGGSGGSYGGGSSSGGGSGGRGGSGGSYGGGSGSGGGSSGSYGGGSSSGGGSGGSHGGGSGGSYGGGSSSGGGSGGRGGSGGSYGGGSGSGGGRGGGCEEGSGSGGRSGGSYGGGSGSGGGSSCSYGGGSSSGGGSGGSYGGGSSSGGGSGGKGGSGCSYSGGSSSGGGSGGSYGGGSSSGRGSGGRGGSAGSYGGGSGSGGGRGGGCEEGSGSGGRSGGSYGGGSGSGGRSGGSYGGGSGSGGGSGGSYGGGSGSKGGSGRSSQVQS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues The designed protein sequence is ",
    "response#1": "EKTTMQDLNSRLASYLDKVQALEDANKELESKIREWYDKQGSRTFHRDYSPYYDTIEDLKNQIVNITADNNKTLLDLDNTRMTLDDIRMKCDIENNLRLAVNADINGLRKVLDDLTMQKSDLEMHYESLQEELIALKKNHEDEMSQLTGQNSGDVNVEMNAAPGRDLTKILNDMREEYERISAKNRKDIEEQYETQMSQMEQEVMSSGQEMESNHKEVTQLRHSIQEMEIELQSQLSKKSALEKSLEDTKNHYCGQLQQLQEQIRSLEGQITEIRGEIECQNQEYSLLLNIKTRLEQEIKTYRSLLEGGQEDNS",
    "response#2": "NEKVVMQNLNSRLASYMDKVQELEEDNANLEKQIQEWYSRKGNRVFQKDYSHYYNTIEDLKDRIVDLTARNNKALIDMDNTRMTLGDFRVKLEMEQSLPQGVDADINGLQKVLDDINMEKSDLEIQFDSLDDELKALKKSHKEEMNQLTGLNDGDVNVEINVAPSTDLTQVLNDMREEYEHLISKNRQDIEQHYESQMTQIEHQLTNSGPEMETNMKQVSQLQHSVQELNIELQTQLTTKSALEKALEDTKNRYCGQLQQIREQISEMEAQLAQVRAETECQNQEYGLLLSIKTRLEKEIETYRKLLEGGQQQ",
    "response#3": "EKTTMQDLNSRLASYLDKVQALEDANKELESKIREWYDKQGSRTFHRDYSPYYDTIEDLKNQIVNITADNNKTLLDLDNTRMTLDDIRMKCDIENNLRLAVNADINGLRKVLDDLTMQKSDLEMHYESLQEELIALKKNHEDEMSQLTGQNSGDVNVEMNAAPGRDLTKILNDMREEYERISAKNRKDIEEQYETQMSQMEQEVMSSGQEMESNHKEVTQLRHSIQEMEIELQSQLSKKSALEKSLEDTKNHYCGQLQQLQEQIRSLEGQITEIRGEIECQNQEYSLLLNIKTRLEQEIKTYRSLLEGGQEDS"
  },
  {
    "reference": "MYRVVRRLLFLTPPERIHTLVFAMLRCVTSIAVLRRLLRWVLGPTDPVLASTVFGVRFPGPLGLAAGFDKDGMGLLAWGALGFGYAEVGTVTAYPQPGNPAPRMFRLPADRALLNRMGFNNNGAGALAIQLAHHRPEVPIGVNISKTKATPASHTVDDYRASARLVGPLASYLVVNVSSPNTPGLRDLQAVESLRAILLGVLAETSVPVLVKIAPDISDSEIDDITDLAVELRLAGIVATNTTVSRDCLVTPGIDALGAGGISGPPVARRAVEVLRRLYGRVGDRLVLISVGGIETADDAWDRITAGASLLQGYTGFIYGGGFWPKHIHDGIARRLHDGGFASLRDAVGSATAKSE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must have high specificity towards dihydroorotate dehydrogenase (quinone) activity.2. The Nucleophile active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "DVGALTPPLWGFEEREKLMVFYERASGARLHAAYFRPGGVHQDLPPALIDDIETWAHEFPRVIDDIDGLLTENRIFKQRNADIGVVTEEDILEYGFSGVMVRGSGLAWDLRRAQPYERYDEFDFQIPIGKNGDCYDRYLVRMEEMRQSLSIILQAIAKLRAPEGQGDVLARGKITPPSRSDMKTSMESLIHHFKLYTEGFHVPAGEVYACVEAPKGEFGVYLVADGTNKPYRAKIRAPGFLHLQAMDHMSRGHQLADVAAIIGTMDVVFGEIDG",
    "response#2": "DVGALTPPLWGFEEREKLMVFYERASGARLHAAYFRPGGVHQDLPPALIDDIETWAHEFPRVIDDIDGLLTENRIFKQRNADIGVVTEEDILEYGFSGVMVRGSGLAWDLRRAQPYERYDEFDFQIPIGKNGDCYDRYLVRMEEMRQSLSIILQAIAKLRAPEGQGDVLARGKITPPSRSDMKTSMESLIHHFKLYTEGFHVPAGEVYACVEAPKGEFGVYLVADGTNKPYRAKIRAPGFLHLQAMDHMSRGHQLADVAAIIGTMDVVFGEIDA",
    "response#3": "PADLRLTITPPGSKSISNRALILAALGSGSCRIRNLLHSDDTEYMLSAVSTLGGTSYSWEDGGQVLVVEGNSGKLKASDEPLYIGNAGTASRFLTTVAALCAPTSTATTTVLTGNARMKLRPIGPLVDALRTNGVAIDYLEREKSLPLRINASNGLEGGEIELAATVSSQYVSSILMAAPYAKKAVTLRLVGGKPISQPYIDMTISMMKSFGIHVTPSADEPDTYHIPQGVYQNPAEYTVESDASSATYPLAVAAITGTTCTIPNIGSKSLQGDAQFAVKILEPMGCEVHQDDYTTTVTGPKSGSLKALPSVDMTTMTDAFLTATVLAAVAEGTTEITGIANQRVKECNRILAMKDQLAKFGVVCNEL"
  },
  {
    "reference": "MGGKWSKSRESEWQDVRKRMKQVEPAAEGVGAASRDLEKHGAITSSNTAANNAACAWLEAHEDEEVGFPVRPQVPLRPMTYRAAWDLXHFLKEKGGLEGLVWSQKRQDILDLWVYHTQGFFPDWQNYTPGPGTRLPLTFGWCFKLVPVAPDTVEEANEGENNCLLHPMSLHGMEDPEKEVLVWRFDSQLAFHHLARELHPEYYKDC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that can perform GTP binding in host cell plasma membrane, extracellular region, host cell Golgi membrane to support suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host autophagy, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I.2. The protein design should include a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif to ensure proper function.3. The protein must exhibit the following characteristics: Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis. The designed protein sequence is ",
    "response#1": "MGGKWSKRSVPGWNTIRERMRRAEPAAEGVGAASRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIHSQKRQEILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVEPDQVEEANVGENNCLLHPMSLHGMEDEEGEVLMWKFDSRLAFHHIAREKHPEFYKDC",
    "response#2": "MGSKWSKSWPAVRERIRRTEPAAEGVGPASRDLDKYGALTSSNTAANNADCAWLEAQEEPEEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLEGLVYSKKRQEILDLWVYNTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVDPREVEEANKGENNCLLHPMSQHGMEDEDREVLKWKFDSSLARRHIAREMHPEYYKDC",
    "response#3": "MGGKWSKRSGAGWSTVREKMRRAEPAAEGVGAASRDLEKHGALTTSNTAANNADCAWVEAQEEEEEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLEGLIYSKKRQDILDLWVYNTQGFFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPREVEEANEGENNCLLHPMSQHGMEDEDREVLKWQFDSSLARRHLARELHPEYYKDC"
  },
  {
    "reference": "MGSRALLLLKVALPLKHQIKCLNYTKITPASELLTKRRFSIKATNGSSLQPPDVSRLAETARISLNQNEVEEFAPKIRQVIDWFGQLQAVDLNSVEPAIRADSEGENLRDDVPETFSEREAIIAAVPSYEKPYVKVPKVLNKE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein have enhanced ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) +   L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate.2. The protein must exhibit the following characteristics: The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln)..3. The designed protein should be involved in glutaminyl-tRNAGln biosynthesis via transamidation, regulation of translational fidelity by localizing to mitochondrion, chloroplast, glutamyl-tRNA(Gln) amidotransferase complex and exhibiting glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity, ATP binding. The designed protein sequence is ",
    "response#1": "VIGLEVHAQIQTNSKLFSGAEVAFSSPINSCVSLFDASIPGTLPVLNRKCVELGVRTALALGCKVNSVSMFDRKHYFYADLPAGYQITQQRAPLARGGLLSFPVFVPGVSRKPYYKSARLHQLQLEQDSGKSLHDPVERKSLVDLNRAGVPLMELVFEPDLETGEEAAALVKELILILTRLGSCSCRMEEGALRVDANISVHKENTPLGTRTEVKNIGSVRAVAQAIEFEIERQIGILDGGGKIFNETRAWDAAGRQTIAMRDKEVVQDYRFMPEPNLPPLHVEALLQEVHSKLPELPQLFKSVADRHHSGKLANNWFIHEFLGAFTKINVKVDLDILPANFLADLVLQVAEKNISTTSARLLLLQIIQTPEDKGKPIQDLIVQYDLGSPVDMSTEDLNDAVSDICSEIISNNADVVERIKKGQKNSIKFLIGLAMKETQGKVNAKTFSEKFNELLA",
    "response#2": "VIGLEVHAQIQTNSKLFSGAEVAFSSPINSCVSLFDASIPGTLPVLNRKCVELGVRTALALGCKVNSVSMFDRKHYFYADLPAGYQITQQRAPLARGGLLSFPVFVPGVSRKPYYKSARLHQLQLEQDSGKSLHDPVERKSLVDLNRAGVPLMELVFEPDLETGEEAAALVKELILILTRLGSCSCRMEEGALRVDANISVHKENTPLGTRTEVKNIGSVRAVAQAIEFEIERQIGILDGGGKIFNETRAWDAAGRQTIAMRDKEVVQDYRFMPEPNLPPLHVVEQIEAAKAELPELPAVKDNIATVSPNSEEETPLTTTCGSNFLANYRSPFEATIVSQLRSRGALLVGKTNLDEFGMGSHSVHTAFGAVAQEGEHEQQAVKHSAGGSSGGSAVAVATGEADIALGTDTGGSVRLPAGYTGVVGFKPSYGMLSRYGVVPYANSLDTVGLLAKEVRPIAELILGGADKVTRGLWAEHDPLDPTSLSHGARRRCASQRDCYTGPLPKDPYPLKNLKFGLPLEYNITELSPSIRHSWSAAAAKLQSLGARLVPVSLPSTRHALSAYYVIAPAEASSNLAKYDGIRYGTRFTSPTESDAAISEGPEGREEEEEGILYARARTAGFGDEVKRRILLGAYTLSSAAMDNYFLKAQKVRRLVRRDFNRVFALPNPLLDKPERFELSELPETVGLEDKWGPTEVDFLLCPTAPTTAPRLDEVLSEEEKDPVSAYMNDVFTVPASLAGLPAISVPMRVEDKEGAGMAGLQLIGQYWDDARL",
    "response#3": "AVKDNIATVSPNSEEETPLTTTCGSNFLANYRSPFEATIVSQLRSRGALLVGKTNLDEFGMGSHSVHTAFGAVAQEGEHEQQAVKHSAGGSSGGSAVAVATGEADIALGTDTGGSVRLPAGYTGVVGFKPSYGMLSRYGVVPYANSLDTVGLLAKEVRPIAELILGGADKVTRGLWAEHDPLDPTSLSHGARRRCASQRDCYTGPLPKDPYPLKNLKFGLPLEYNITELSPSIRHSWSAAAAKLQSLGARLVPVSLPSTRHALSAYYVIAPAEASSNLAKYDGIRYGTRFTSPTESDAAISEGPEGREEEEEGILYARARTAGFGDEVKRRILLGAYTLSSAAMDNYFLKAQKVRRLVRRDFNRVFALPNPLLDKPERFELSELPETVGLEDKWGPTEVDFLLCPTAPTTAPRLDEVLSEEEKDPVSAYMNDVFTVPASLAGLPAISVPMRVEDKEGAGMAGLQLIGQYWDDARLLQAAEMIEA"
  },
  {
    "reference": "MAEKKGSSSEKNLYCTFCGKSQHEVKKLIAGPSVFICDECIDLCNEIIRDEQPSTEAKDGRGDLPTPAEIKANLDNYVIGQDLPKRTLAVAVYNHYKRLRHKDKAGKDEIELAKSNILLIGPTGSGKTLLAQTLARMLNVPFVMADATTLTEAGYVGEDVENIIQKLLQSCEYDVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTMASVPPQGGRKHPNQDFLQIDTTNILFICGGAFAGLEKVIENRTEASGIGFGATVKSKKQRSISEVFQEIEPEDLIKFGIIPELVGRMPVVTALAELGEDALVQILTEPKNALVKQYSKLLAMEGVELEIRPAALKAIARKALARKTGARGLRSILEQSLIGTMYELPNADNVEKVVVDESTIEDGKAPLLVYREAAKKA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein contains novel ClpX-type ZB domains that confer a unique function or activity.2. For general function, the protein need meet that It directs the protease to specific substrates; Can perform chaperone functions in the absence of ClpP.3. The designed protein should have a binding site that is specific to the target ATP, Zn(2+) ligand.4. The designed protein must be optimized for ATP hydrolysis activity, protein dimerization activity, zinc ion binding, unfolded protein binding. The designed protein sequence is ",
    "response#1": "QKSNILMLGPTGSGKTLLAQTLAKIINVPFAIADATSLTEAGYVGEDVENILLKLIQAADYDIERAEKGIIYIDEVDKIARKSENPSITRDVSGEGVQQALLKILEGTVASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIDSIIQKRTSKKSMGFGAEIESKQVLDLGSLLKQIQPEDLLKFGLIPEFVGRLPDF",
    "response#2": "KSNILLIGPTGSGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVENIILKLLQSADYNVDRAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLMEGTVASVPPQGGRKHPQQEFLQVDTTNMLFICGGAFAVWTRSFRRVARDRASASVQMCVLMTSVVLGAILQSVEPEDLLKFGLIPEFIGRLPVLFKSNILLIGPTGSGKTLLAETLARLLDVPFTIADATTLTEAGYVGEDVENIIQKILQKCDYDVEKAEQGIVYIDEIDKISRKADNPSITRDVSGEGVQQALLKLIEGTVASVPPQGGRKHPQQEFLQVNTANILFICGGAFAGLEKIIRSRSEQGGIGFAADVKSKDDSRNVGEVLADVEAEDLIRYGLIPEFVGRLPDLDEAALIEILTKPKNALVKQYGRLFEMEGVSLSFTDDALKSVANRAIARKTGARGLRSILEGILLGTMYDLPGMENVEEVVINGEVAEGRAALDEAALIEILTKPKNALVKQYGRLFEMEGVSLSFTDDALKSVANRAIARKTGARGLRSILEGILLGTMYDLPGMENVEEVVINGEVAEGRAAD",
    "response#3": "KSNILLVGPTGSGKTLLAQTLARTLDVPFVIADATTLTEAGYVGEDVENIIQKLLQNCNYEVERAQKGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTMASVPPQGGRKHPNQDFVQIDTTNIMFICGGAFDGLAKIISERSEKSGIGFSASVKSREERTASQVMMDTEPEDLIKFGLIPELVGRLPXLXFXPXXK"
  },
  {
    "reference": "RKHYWAQNRALWYTRVCMTILTCLPAAVLSFSHLSSNLCKWCEASSPLCKDNICLSNCSLSSFCTATEEICIAIWRKINETMTVQTMCHHPALPLEGVDPSLLLNSTSRECHMVPQPAEEGAMQVCSCQGEHECNDKLIFDKGPNGFSKLQSKDVIPVVVVSLVPPILVALVATATFYLYRTRHPGKPGPPSRPDWPTKRTTDRYHALDLARREGGGGVMMPELEREESNTKLPSPAHDIISDILEGQGQMAEPLPIKLEALVGKGRFAEVWRGRLLHGEKGGVSSYETVAVKVFPAVEYASWRNERSIFSDPKLEHDNVVRFLAAEERGPPGHALRKYWLVLAYYSLGNLQDFLSANILSWEELVAMAGSIARGLAHLHSDTTVNRVPKVPVAHRDLKSSNIVVKSRRECALCDFGLALRLDLSLTVDDYANSGQVGTARYMAPEVLESRVNLEDLEAFKQMDVYSMALVLWEMASRCHAIGEVKSYEPAFGSRVCEQPCVDSMRDLVLRDRGRPDIPTTWTQHQGMNVFCATITECWDHDPEARLTAHCVVERFNALQQEEEEKEEEEEEEEEGRPEESCQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways.2. The protein can be targeted to plasma membrane, cytoplasm and perform metal ion binding, ATP binding.3. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "IKLEDVVGRGRFAEVWRARLLQSDMGGVNSYETVAVKVFPNVEYASWRNECSILSDPTLQHENLVQFLAAEERSLPSDTLRTYWIVLAHYSLGNMQDHLTANTLSWEELVSLASSIANGLAHLHSETKLNGAPKVPVAHRDLKSSNILIKNRKQCVLCDFGLALRLDISLTVDDYANSGQVGTARYMAPEVLESRVNLEDLEAFKQMDIYSMALVLWEMASRCDVIEEVKAYQPAFGSKVCEQPCVDSMRDLVLRDRGRPDIPSAWTQHQGMSVFCSTITECWDHDPEARLTAHCVVERNMCKWCEASIPMCKDSVCFSNCTLSSFCEVAEEICVAIWRKNNDTMTVQTMCHHRTLPLEGVDPNLLVNLTRRECHMVPQPSEHGDMMVCGCQGEHECNDKLIFDKGANGFL",
    "response#2": "NMCKWCEASIPMCKDSVCFSNCTLSSFCEVAEEICVAIWRKNNDTMTVQTMCHHRTLPLEGVDPNLLVNLTRRECHMVPQPSEHGDMMVCGCQGEHECNDKLIFDKGANGFR",
    "response#3": "QLCMFCDLELSSCSGTGTCMSNCSITSTCDDSNEVCVSIWRKNEKGFSIETLCHDPSKPLYGVMLEDYNSSTCVMKEKNTTSGMAHFCSCTDEEECNDKMLFSPIL"
  },
  {
    "reference": "MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELARLGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNVDIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYAINFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKEAIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNETEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAVVKNTAGAGDAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRIQSIHYQQTIFTH",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein with ribokinase activity, DNA binding is needed to enhance D-ribose catabolic process in a specific context.2. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.3. The H-T-H motif domain within the designed protein should be capable of recognizing and binding to a range of DNA sequences.4. For general function, the protein need meet that The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.; Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium The designed protein sequence is ",
    "response#1": "ILGSFNLDVVTEVERFPSVGETVRAISTRFYPGGKGANQAIAASELNSHVHLFVKVGDDAFGTDAEKYFSSTKIGSYTLIKDIKTKTGSALVMVENLTGNNSIIIDQGANATITKSEILSDINNLKSAKIFLTQLENNMDITLFAVEMAKRCGCYVIINPAPYSQDIMPYLSSVDLLIPNGTEAELISGIKISNLHSAEQAIRVIHALGVKEIIMTLGDNGALYFNGSVMKHYNAINASVVDTSGAGDAFTGALAARLVEGRNMDEAVKFAVAYASLAVEKKGASV",
    "response#2": "SHVCVIGSFNVDIISYLPRLPAVGESLLANKFIFSPGGKGCNQALAASYADSDVHFITKVGADHFSEYAINFMNASKIHKSIIYQTQGTQTGTATILVNEDTGDNVIAIYPGANMTITPDEVVTIINANIVLLQLETNYSALRQAIALAQKNSIPVIINPAPYNDGVNSIIENIDYITPNETEAGLLAGIEVTDIASAREAAKIIHQKGVKTTIITLGSKGSLAYDGRKFIYSPAFPAVVKNTAGAGDAFNGALASGLAKGKPLESALCYASAFASLAVETANASDSIYILGSFNIDIVSETTTFPQIGQTIRASATRFYAGGKGTNQAVAAAKINNNVHLTVKLGADEFSKKAKRYLTNTEISSFSFFENEMSSTGIAMVWVSGATGDNMISIDLGANETFTQEDILLDIDTIRNCKVFLTQLENNFPITRFAIMQAKLGKSLVVLNPAPYVSEIKEILHLIDIITPNKIEAEALSGINIVDIYSAKNAARSIYVQGAKCVIITLGSDGCLIFDGTVFTHIPAYKSAVVDTSGAGDAFTGALAACIANGKGLINAAQYASAFASLKVERKGASNC",
    "response#3": "SHVCVIGSFNVDIISYLPRLPAVGESLLANKFIFSPGGKGCNQALAASYADSDVHFITKVGADHFSEYAINFMNASKIHKSIIYQTQGTQTGTATILVNEDTGDNVIAIYPGANMTITPDEVVTIINANIVLLQLETNYSALRQAIALAQKNSIPVIINPAPYNDGVNSIIENIDYITPNETEAGLLAGIEVTDIASAREAAKIIHQKGVKTTIITLGSKGSLAYDGRKFIYSPAFPAVVKNTAGAGDAFNGALASGLAKGKPLESALCYASAFASLAVETANASDTC"
  },
  {
    "reference": "MEPVDPNLEPWKHPGSQPTTPCSKCYCKKCCWHCQLCFLKKGLGISHGRKKRKHRRGNPQSRRDHQNPVPEQPRPIIRGNPTGPKESKKEVASEAETDP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive; Through its interaction with host TBP, Tat may also modulate transcription initiation; Other factors such as HTATSF1/Tat-SF1, SUPT5H/SPT5, and HTATIP2 are also important for Tat's function; Tat can reactivate a latently infected cell by penetrating in it and transactivating its LTR promoter; Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation; This also contributes to the increase in proviral transcription rate, especially when the provirus integrates in transcriptionally silent region of the host genome.2. The protein design should emphasize Basic residues compositional bias.3. The protein's functional design necessitates the inclusion of a Nuclear localization signal, RNA-binding (TAR), and protein transduction motif. The designed protein sequence is ",
    "response#1": "MEPVDPSLEPWKHPGSQPKTACTPCHCKKCCFHCQVCFITKGLGISYGRKKRRQRRRTPPDSKNHQVSLPKQPSSQPRGDPTGPKEPKKKVESKTETGPCD",
    "response#2": "MENRWQVMVVWQVDRMRIRTWNSLVKHHIHVSKKAKDWVYRHHYDSRHPRVSSEVHIPLGDARLVIKTYWGLHTGERDWHLGQGVSIEWRQRAYTTQIDPDLADQLIHLHYFDCFSDSAIRKAILGQVVRRKCEYPSGHNKVGSLQYLALVALTTPKKIKPPLPSVRKLVEDRWNKPQKTRGRRGNHTMNGH",
    "response#3": "MEPVDPNLEPWNHPGSQPTTACSKCYCKFCSYHCLVCFQKKGLGISYGRKKRRQRRGTPQSSKDHQNPISEQPLPQTQGVSTGPEESKKKVESKTKPDPFN"
  },
  {
    "reference": "MDLFQLTAHQLHQLLTKKEISALDITEAVYNRIEQVEDKVKSYLTLTREQAEIKARQVDQKIAAGESIGPLAGIPIAIKDNMCTQGIRTTCASKILYNFVPPYSATSVEKVYTADMVMVGKTNMDEFAMGSSTENSGFHLTHNPWDLDRVPGGSSGGSAAAVAAGEAIISLGSDTGGSIRQPAALCGVVGMKPTYGSVSRYGLVAYASSLDQIGPFTRDVTDMAHVLNVICGHDPMDSTSANLKQTDYTQFLTNDIKGMKIGVPREYMADGIDPQVREKIKAAIQKLTELGAHVEETSMPHTDYAMPAYYLIATAEASSNLARYDGVRYGLRVEEARDLVDMFMRSRSQGFGDEVKRRIMLGTYSLSAGYYDAYYLKALKVRTLIKQDFDRAFEKYDALLSPTSPTTAFKIGEMVNDPIQMYLQDVCTIPVNLAGIPAISLPCGLANNLPVGLQLMGKAFDEGTLLRIAYTLEQNTEYTRLRPEI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein can be targeted to glutamyl-tRNA(Gln) amidotransferase complex and perform ATP binding.2. The Charge relay system active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The protein must exhibit the following characteristics: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The designed protein sequence is ",
    "response#1": "DVTTACLDRITATEPHLAALLYVNAENALARATTLDSEGPDAARPLWGVPVTLKDAFSTRGMPTTAGSRMLEGYTPFYDAFAVQKLHEAGAVILGKTNLDEFAMGSSTENSAFKVTRNPWNLNKVPGGSSGGSAASVTAGQCFASLGTDTGGSIRQPASFCGCVGLKPTYGRVSRYGVIAYGSSLDQVGPLTRSVEDCARVLTAIAGYDSRDNTCDPRPAEDYAATLSSRPLKGARLGIPREFYGQGLSDEVRAACEAAIQAARNQDAELVEVSLPHTDAAIATYYIIAMAEASSNLARFDGVRFGHRSADIKNLDDLYVHSRTEGFGPEVKRRIMLGAYVLSSGYYDAYYRKAAQVRRLIRDELLAALGQCDALLAPVSPVTARDLGGNTADPLQIYLMDAYTLSLNLAGLPGLSLPVGLGAKSGMPVGMQIIGKPFEETQVLQVADLLE",
    "response#2": "EVAQAYLKRVQELDPGLGAFLSLNERLLEEAEAVDPGLPLAGLVVAVKDNIATRGLRTTAGSRLLENFVPPYEATAVARLKALGALVLGKTNLDEFGMGSSTEHSAFFPTKNPFDPDRVPGGSSGGSAAALAADLAPLALGSDTGGSVRQPAAFCGVYGLKPTYGRVSRFGLIAYASSLDQIGPMARSVRDLALLMDAAAGPDPLDATSLDLPPRFQEALEGPLPPLRLGVVREALAGNSPGVERALEEALKVFRELGLSVREVSWPSLPQALAAYYILAPAEASSNLARYDGTLYGRRAAGEEVEGMMEATRALFGLEVKRRVLVGTFVLSSGYYEAYYGRAQAFRRRLKAEAQALFREVDLLLLPTTPHPAFPFGARRDPLAMYREDLYTVGANLTGLPALSFPAGFEGHLPVGLQLLAPWGEDERLAAYAEAVKA",
    "response#3": "ELTKEYLAKIKEQDKRINSVITLCEAEALKEAEDADAIISAGKQGLLTGIPILHKDLFCTKGIRTTAASKMLDNFVAPYDSTVTKNCKDQGMVTLGKLNMDEFAMGSTNEYSYYGAVSNPWDLERVPGGSSGGSAAAVAAGFAPISTGSDTGGSVRQPASFCGLTAMKPSYGSTSRFGMVAFASSFDQAGVLGHYAEDVAIMLDAIAGECEFDSTCVGVKQNHFTQDLEKDISGKVIGVDESLIKDLPAQIQEAVSKTLDNFKKLGAEIKSVKVPDLKEALSTYYIITPAEAAANLARYDGIRYGYRNPEARDLDELYRKSRTDGFGAEVKRRIMIGNYVLASSQYDSYYNKAQQLRKVMTDQINQIFTQVDAIFMPASPSEAFKKGDKLDPVSAYLSDIYTIPANISGLPAIAFPIGFANNLPVGGQLMAKAFNDNLFSLANTIAEA"
  },
  {
    "reference": "MIFHPRPSPIAAAMYQLRDLGVDAIILHGPSGCCFRTARLLELDGVRVFTSNIDENAIVFGASENLKKALDYAIEYLKKELKKERPMIGIVGTCASMIIGEDLWEFVDDDRAIIIPVEVHSGSGDNTIGAIKAMESALKLGIIDEKEFERQKFLLKKATEVEKKRGMAKKEYIKPTYDDDLNEAIKVLKDLKEKDGKIACVLNAKKETAYLFAHPLIVLNKYFNCVNIANLDINKGLPKIRRDAQNILRRFKADYITGGLDEYPITGERAVEILKDLDVDAIVVSGVPHALPIEEIDKDIIKIGISDGPRTYHPIKEIYDYAIVELDAHAKVLGKRDIVKSRFGEILDYALE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit iron-sulfur cluster binding, ATP binding. The designed protein sequence is ",
    "response#1": "LIFTDSAAAKVKDLLAEEGNPELKLRVFVQGGGCSGFQYGFTFDEAVNEDDTVLDKNGVQLLVDPMSFQYLVGAEIDYKEDLEGAQFVIRNPNASTTCGCG",
    "response#2": "WNNLDYLIIDMPPGTGL",
    "response#3": "GLIFTDSAAAKVKDLLAEEGNPELKLRVFVQGGGCSGFQYGFTFDEAVNEDDTVLDKNGVQLLVDPMSFQYLVGAEIDYKEDLEGAQFVIRNPNASTTCGCG"
  },
  {
    "reference": "MLLSLAQWLQSISPEFGSFRVFQYLTFRAVMAALTALLIGLLAGPFVIRRLISLKIGQPIREYAMQTHLSKSGTPTMGGVLILMSIGISTLLWFDLSNRFVWIVLLVTLGFGAIGWADDWRKVVLKDPEGMRSREKYLWQSLIGLVAALYLVFSISESSNLRVLELFFSWVQSGFDVDLPPKAGLLVPFVKEISYPLGVFGFVILTYLVIVGSSNAVNLTDGLDGLAIMPVVMVGSALGVFAYVTGSAVYSKYLFFPHIPGSGELLIFCSAMAGAGLAFLWFNTHPAQVFMGDVGALALGAALGTIAVIVRQEIVLAIMGGIFVVEALSVMMQVIWFKYTKKRYGEGRRLFKMAPLHHHFEKSGWKETQVVVRFWIITMLLCLVGLSTLKLR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a fast turnover rate for di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-alpha-   D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-   alanyl-D-alanine = di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-   alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-   alanyl-D-alanine + UMP. The designed protein sequence is ",
    "response#1": "VAGTHGKTTTSSMLASILFGADKNPSYLIGGDVVSFNRGATWTNGDLFVVEADESDGTHSQLPLSATILTNVDIDHLDHFKTQAAIEQSFFDYVGNISGPRVLCLDDPVCALIAKTHKSVSYSTNFRSSADFVATEVQFKDASAFFRVVQNIGEQKIELGRVNLPQRGMHNVSNALGAIVMAEEFGVPAEAIGRALSHFSGAEGRMALYRAASGPSVVVDYAHTPDALDNALGALKAHLGSSEGKLWCLFGCGGERDTGKRAEMAAVAERLADHVVITDDNPRFESAEQIRKEIIAGLSPDTPFMELGDRRQAIAYVVQQAGQNDVVLVAGKGHETGQIIGDRVLPFDDVAVARAAIRALEG",
    "response#2": "VTGTSGKTTTSYLIESALRSAGLVTGLIGTVETRLAGTRLESAFTTPEAPDLQALLAVMLEGGVTHVVMEVSSHALVLGRVAGLRFAVGGFTNLSSEHLDFHRDMADYFAAKAMLFDGRCAREVVCTDTEWGSRLVGPETVTVATQGTADWTATDVVTSTTGAQRFTVNAPDGARTEVELRLPGSFNIANALLATAMARELGVSEEAIRRGLAGFAGAERRFDVQGVDSGATFVDDYAHLPNEIAAVLSGVRDESDKWSRVVAVFQPNRFNRMSVMSHLYADAFAAADLVVVSAGNVHKISETLLEDLKAA",
    "response#3": "MATVITGGGTSGHVIPAIAIAELLQDSGVNKDQLHFIGSTNGIETTLVPAAGLALSLLTVQGFRRDLSISALQKNIRTFGVLRKATQHAQRLLASLKPAVVISVGGYVSVPATRAAKKLNIPVVTVSYDRQPGLATRLQARNAAFMAFEGVEAACPVTVLIVGGSRGARPINEAFLQVLHEVEQKPYQCLYVTGDVHYDKVMKAVNDVGNPSNVIIRPFIHNMPEVLAGVDVIVARAGATTLAEITALGIPSILIPSPYVTNNHQEKNARALEKKGAAIVRLESELTGVRLLDDIDRILLNEQTLTNMKEAAFQLGIRDAAERLSALARDA"
  },
  {
    "reference": "MAETRIHPSAVVDPAAQVGEGCEIGPFCVIGPEVGLGRGVVLKSHVVVAGETLIGDETVVFPFASLGEVPQDLKFRGERTRLEIGARNRIREYVTMNPGTEGGGGVTRIGDDGLFMAGSHVAHDCQIGNRVILVNNASVAGHCVLEDDVIVGGLSGVHQFVRIGRGAMIGAVTMVTADVIPFGLVQGPRGHLDGLNLVGLKRRGASREEIHALRDMLAQLGQGSFRDTARHLAEAENGPMVREVLDFILGPSDRSFLAPHP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in lipid A biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.2. The protein should be optimized for activity in Glycolipid biosynthesis, lipid IV(A) biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "DIPPFTIGTGNPYALGGINKVGLQRRQVSFETRLALIKTFKRVFRSDESFQASLESVLEDFGEVPEVRHFVEFCRQPSKRG",
    "response#2": "GAVVANHTGWLDIFTLNACQRLYFVSKDEVADWPFIGWLARATGTVFIRRDPREAKAQQALLEDRIRDGHHLLFFPEGTSTDGLQVLPFKTTLFAAFYTHGLDKVMQIQPVTVNYR",
    "response#3": "DIPPYCLAEGNRATLRGLNLTGLRRHIPREEINALKSAYRELFEEGKALQDVAQRLFEESSSEKVKNLCKFIKTSKRGL"
  },
  {
    "reference": "MKNFLKNKKVLLGISSSIAVVKIPKLIEILSKKGAEIEVVATKNALKLIDVKEIEKVLNKKVWIDMFDDKKGYPHIDLAKWADLILLAPATANLIGKICSGLADDLLTTIMLVFQGNVFVAPAMNFRMWNNFFVQENLNKLIETQFKILEPQEGKLACGEEGMGRMMEVADIVFNIEKFLTNKNLLVGKTILITVGATREYLDPVRFISNGSSGKMGMALVKAAVSMGGKVILIRGQCLREMYSGAFVRGDDQLKIFNVESGEEMFEAVKANIDQADIFISAAAICDFKPEKISKQKIKKKNEILNIKFQKTVDILEWVGRKCKIKNTSPTCPNDLFGQKDLFWREKLKIIGFALESENLIENARKKLLFKNLDMIIANQPENIGAQEGEFWFIDKTTEIFKKGRKEDIAIEILKKISQQSIDFLENKG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein with metal ion binding, phosphopantothenoylcysteine decarboxylase activity, FMN binding is needed to enhance coenzyme A biosynthetic process in a specific context.2. The designed protein must have at least one Flavoprotein domain.3. The CTP binding site should be located in a region of the protein that is accessible to the ligand.4. A key design requirement for the protein is the optimization of the Proton donor to achieve high catalytic efficiency for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.5. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.6. Ideal protein should have a binding affinity for key enzymes in the Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MKVALGVCGGIAAYKACEVVRLLQDAGIRIQVVMTAAAQEFVRPLTFAALSGEKVITGLWQSGGEQPNFDSAIDHISVAQSIDALIVAPCTADTAAKFAHGQADDFLSTLYLATPAPVIIAPTMNVEMLNHSATQANLETLRQRGVHIVEPGSGYLACGMVGAGRLAGPEEIVAAVLAAAREVLAAGETLAGRLVVISAGGTRERIDPVRYLGNDSSGKQGMALAEEALARGAEVVVVAGHLDVPAPRGAEVISARYTESMRQAITSLADEADVVIMAAAIADYRPANQTTTKIKKIDEGDETTLRLVSNPDILSEISHRPDRGNVVVGFAAETASGAALLELGRAKIRRKGCDLLAVNEVGEEKGFGRDENTVSVLWSGGEQNMGAVPKAEVADALWDAVVAHLGG",
    "response#2": "MKVALGVCGGIAAYKACEVVRLLQDAGIRIQVVMTAAAQEFVRPLTFAALSGEKVITGLWQSGGEQPNFDSAIDHISVAQSIDALIVAPCTADTAAKFAHGQADDFLSTLYLATPAPVIIAPTMNVEMLNHSATQANLETLRQRGVHIVEPGSGYLACGMVGAGRLAGPEEIVAAVLAAVQAVLAAPGRLAGRRVLVTSGPTHEPIDPVRYIANRSSGAQGTAIARALAALGAEVIFVTGPASVPPPEGVEIVPVQTAQEMADAVQAALPVDAAVFAAAVADWRMAQASERKLKKTRDGMPVLRFAENPDILAGVAQMEQGRPALVIGFAAETDDVVAHATAKRVRKGCDWIVANDVSPATGIMGGSENAVVLAALWEGGRPLTGRHVLVTSGPTHEPIDPVRYIANRSSGAQGTAIAEALSKLGADVSFVTGPATAPRPRGPRITEVETAREMLTAVEAALPADAAIFCAAVADWHVVNAGPSKMKKEAGGLPALDFAENPDILATIARRNENRPRLVVGFAAETDDVVAHATAKRARKGCDWIVANDVSPETGIMGGGHNAVTLVTAAGIEDWPEGDKAAVATRLAALVAERL",
    "response#3": "MKVALGVCGGIAAYKACEVVRLLQDAGIRIQVVMTAAAQEFVRPLTFAALSGEKVITGLWQSGGEQPNFDSAIDHISVAQSIDALIVAPCTADTAAKFAHGQADDFLSTLYLATPAPVIIAPTMNVEMLNHSATQANLETLRQRGVHIVEPGSGYLACGMVGAGRLAGPEEIVAAVLAFLEAPDAAVALAGRRVLVTSGPTHEPIDPVRYIANRSSGRQGHAIAAAAAAAGAAVTLVSGPVAIPDPPGVTVVRVETAREMLAAVRAALPADVAVFTAAVADFRPAEARGSKIKKDGAAPAPLGLVENPDILATIAGLASGRPGLVVGFAAETDRVVDYARAKIARKGCDLLVANDVSLAGGVMGGVRNTVHLVTADEVEELPREDKTALAHRLVARIAHRLGG"
  },
  {
    "reference": "MANKELKRGLGARHIQMIALGGTIGVGLFMGSASTISWTGPSVLLAYAICGIFIFFIMRAMGEMLYVEPSTGSFATFGHQYIHPMAGYITAWSNWFQWIIVGMSEIIAVGSYTKYWFPDLPAWIPGIVAMVILGAANLISVKSFGEFEFWFAMIKIVTIILMIIAGIGIIFFGFGNGGDAIGLSNLWSHGGFFAGGFSGFFFALSLVIAAYQGVELIGITAGEAKDPQNTLRNAIQSIIWRILIFYIGAIFVIVTVYPWDELNSLGSPFVSTFSKIGITAAAGIINFVVITAAMSGCNSGIFSAGRMLYTLGVNGQAPKFFKKISRNGVPLYGTIAVLIGLAVGVVLNYIAPPKIFVYVYSASVLPGMIPWFIILISHIGFRKAKGAALDKHPFKMPFAPFTNYLTIAFLLMVLVGMWFNDDTRISLIVGVIFLALVVISYYVFGIGKRTQANLTKSEQAAE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The protein must exhibit the following characteristics: Is also active as a minor serine permease..3. The protein should have a specific function in amino acid transport and should be localized to plasma membrane in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "FKDAGKPVIAYADNYSQGQYYLASFADEIYLNSIGSVDIHGLSQENLYFKEMLDKLAVTPHIFRVGTYKSAVEPFLRNDMSAEAKANMQRWLGEMWNNYVLSVSENRNIKKDRILPNAKQYLAELKALKGNSTAYAQQRGLVTDVVTRLDLDKKA",
    "response#2": "FKDAGKPVIAYADNYSQGQYYLASFADEIYLNSIGSVDIHGLSQENLYFKEMLDKLAVTPHIFRVGTYKSAVEPFLRNDMSAEAKANMQRWLGEMWNNYVLSVSENRNIKKDRILPNAKQYLAELKALKGNSTAYAQQRGLVTDVVTRLDLDKKM",
    "response#3": "FKDAGKPVIAYADNYSQGQYYLASFADEIYLNSIGSVDIHGLSQENLYFKEMLDKLAVTPHIFRVGTYKSAVEPFLRNDMSAEAKANMQRWLGEMWNNYVLSVSENRNIKKDRILPNAKQYLAELKALKGNSTAYAQQRGLVTDVVTRLDLDKKK"
  },
  {
    "reference": "MKYREIILNIPKEIAEDFTTFLDEIGVAGYYEILFDREVPRAPHEEIISDDTKFRVYLAQEDNENETKILIYLKANAGEVFFSESRWIETKEYEEAYKEFYKPFIVGSYRVIPTWEKDTALGTTPEGIFPLFINPGLAFGTGHHETTRLVLGRMGNLSLSGKKVVDVGTGSGILSVAAAKSGASPILAVDVDPNSVRSASFNRDENDISSEVLAVEEGGFDHEKIQGQTWDLLIANITFAVLKANIQKIVSIKTDHFLFSGVITERLEEFLELLKNEVGGEGVFFQEDTGWELIEWKRKG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be expressed specifically in the cytoplasm2. The S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions.3. The protein should have a fast turnover rate for L-lysyl-[protein] + 3 S-adenosyl-L-methionine = 3 H(+) +   N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-   homocysteine. The designed protein sequence is ",
    "response#1": "LFQFL",
    "response#2": "MENQYLNVADNSGARELMCIRIIGASNRRFAHIGDVIIGVVMKALPGMSMK",
    "response#3": "GADPG"
  },
  {
    "reference": "NLIQLSNMIKCAIPGSRPLFQYTDYGCY",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. We are looking for a protein with broad substrate specificity for a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-   glycero-3-phosphocholine + a fatty acid + H(+).2. The designed protein must be optimized for metal ion binding.3. The designed protein should have a high specificity for Ca(2+) binding, with minimal off-target effects.4. The presence of Ca(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MSSSGDLDLRLGFDLCDGEQEFLDKRKQVASKALQRVMGLSEALHCDQVPVVAVLGSGGGTRAMTSLYGSLAGLQELGLLDAVTYLSGVSGSSWCISTLYRDPSWSQKALQGPIKYASERVCSSKIGMLSPKQFEYYSREKRAWESRGHSMSFTDLWGLIIEYFLNQEENPAKLSDQQETVSQGQNPYPIYASINVHKNISGDDFAEWCEFTPYEVGFPKYGAYVPTELFGSEFFMGRLLHFWPEPRICYLQGMWGSAFAASLYEIFLKLGGLSLSFLDWHRGSVSVTDDWPKLRKQDPTRLPTRLFTPMSSFSQAVLDIFTSRITCAQTFNFTRGLCMYKDYTARKDFVVSEDAWHSHNYGYPDACPNQLTPMKDFLSLVDGGFAINSPFPLVLQPQRAVDLIVSFDYSLEGPFEVLQVTEKYCRDRGIPFPRIEVDPKDSEDPRECYLFAEAEDPCSPIVLHR",
    "response#2": "MSSSGDLDLRLGFDLCDGEQEFLDKRKQVASKALQRVMGLSEALHCDQVPVVAVLGSGGGTRAMTSLYGSLAGLQELGLLDAVTYLSGVSGSSWCISTLYRDPSWSQKALQGPIKYASERVCSSKIGMLSPKQFEYYSREKRAWESRGHSMSFTDLWGLIIEYFLNQEENPAKLSDQQETVSQGQNPYPIYASINVHKNISGDDFAEWCEFTPYEVGFPKYGAYVPTELFGSEFFMGRLLHFWPEPRICYLQGMWGSAFAASLYEIFLKLGGLSLSFLDWHRGSVSVTDDWPKLRKQDPTRLPTRLFTPMSSFSQAVLDIFTSRITCAQTFNFTRGLCMYKDYTARKDFVVSEDAWHSHNYGYPDACPNQLTPMKDFLSLVDGGFAINSPFPLVLQPQRAVDLIVSFDYSLEGPFEVLQVTEKYCRDRGIPFPRIEVDPKDSEDPRECYLFAEAEDPCSPIVLHR",
    "response#3": "MSSSGDLDLRLGFDLCDGEQEFLDKRKQVASKALQRVMGLSEALHCDQVPVVAVLGSGGGTRAMTSLYGSLAGLQELGLLDAVTYLSGVSGSSWCISTLYRDPSWSQKALQGPIKYASERVCSSKIGMLSPKQFEYYSREKRAWESRGHSMSFTDLWGLIIEYFLNQEENPAKLSDQQETVSQGQNPYPIYASINVHKNISGDDFAEWCEFTPYEVGFPKYGAYVPTELFGSEFFMGRLLHFWPEPRICYLQGMWGSAFAASLYEIFLKLGGLSLSFLDWHRGSVSVTDDWPKLRKQDPTRLPTRLFTPMSSFSQAVLDIFTSRITCAQTFNFTRGLCMYKDYTARKDFVVSEDAWHSHNYGYPDACPNQLTPMKDFLSLVDGGFAINSPFPLVLQPQRAVDLIVSFDYSLEGPFEVLQVTEKYCRDRGIPFPRIEVDPKDSEDPRECYLFAEAEDPCSPIVLHL"
  },
  {
    "reference": "MSRYRGPRFKKIRRLGALPGLTNKRPKAEKDLINQSRFVKKSQYRIRLEEKQKLRFHYGLTERQLLKYIRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMALTIPAARQLVNHRHVLVNGRIVDIPSYRCKPLDIITAKDKQQSRTLIQNSLNSSPHAKIPNHLTLDLFQYKGIVNQIINSKWVGLKINEFLIVEYYSRQIKT",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have structural constituent of ribosome within small ribosomal subunit, plastid to regulate translation.2. For general function, the protein need meet that With S5 and S12 plays an important role in translational accuracy. The designed protein sequence is ",
    "response#1": "MLIKL",
    "response#2": "MEPVGIMVTLRSDRMYAFFDRLINLALPRIRDFRGVNPKSFDGRGNYSLGLREQLIFPEISYDSIDQMRGMDVSIITTATTDEEGRALLKVMGMPV",
    "response#3": "MVPLLLL"
  },
  {
    "reference": "MSISVVILAAGQGTRMRSATPKVLHTISGKPMLFHAIDAAREISTDITVVLYHQAERIREEIETVYQGIEFHIQDAERFPGTGGAMKGIKTRYERTLILNGDMPLVTKSSLEALTAGDAEINMSVITLENPSGYGRVIIEEGNVKEIVEQKDCTPEQLSVKSVNAGVYAVNSELLDRYIPKLSNNNAQAEYYLTDIIKMAVEEEKTVHPVFVEEEEFKGVNSKLDLAHAEEIMQTRIRNHWMREGVIMRLPQTIFIDARATFEGECILENGVSIHGASVLKEAHIKAHSVIEDSVIKESSVGPMGRIRPDSLLEHTHIGNFVEVKKSTLTGVKAGHLAYLGDATIDTGTNIGAGTITCNYDGKKKYQTKIGKNVFIGSDTQLIAPVTIEDDVLIAAGSTITKDVARGELAMSRTPMKTIKNFFYRFFGEK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should take into account the physiological conditions of the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, Nucleotide-sugar biosynthesis pathway.2. A protein that can perform magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity, glucosamine-1-phosphate N-acetyltransferase activity in cytoplasm to support cell wall organization, lipid A biosynthetic process, regulation of cell shape.3. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).4. The designed protein should have a binding site that is specific to the target Mg(2+), UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA ligand.5. The protein requires Mg(2+) to activate a critical catalytic site.6. The designed protein have MobA-like NTP transferase domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "VILAAGMGTRMNSDLPKVLHKVAGAPLLVHAMKSGAALDPKKLVIVAGHGADEVAKAARDWDDRAEVVLQEEQLGTGHAVLQARAALEGFDGDVIVLYGDTPFIQPETIENIAAARARNDVVVLGFLTAEVDDPTGYGRIVRRADGTVARIVEERDASPEERAVREVNTGIYAFDAAVLRDGLSRISTDNAQGELYLTDVLGIARADGARVGAARTADAWLTEGVNDRVQLATLGAELNRRTVERHMRAGVTVVDPRTTWIDADVSLGRDVTIHPQTQLLGASTIGSGVTLGPDTTLTDVEIADGASVSRTQGSLAVIGAGATVGPFAYLRPGTVLGVQGKIGTFVETKNAQIGDGAKVPHLSYVGDAEIGEGTNIGAGTIFANYDGVEKHHTTIGKHVKTGSHNTFVAPVHVGDGAATGGGAVVRRDVPPGALAVSTGPQRNIEDWVRRKRPGTASDEAARSARDSAGAGDE",
    "response#2": "VVVLAAGKGTRMRSSLPKVLHAIADKPMVSHVIDAARQLNAENIYIVYGFGGEVLKATITDNSKSDDLTFIEQKEQLGTGHAVDMASPFIKDDENVLVLYGDVPLTKVSTLQNLLAAKPSDALGMLTIRMDDPTGYGRIVRDADGRIAGIVEEKDATAEQRRIKEVNTGIMVIPNARLKGWLGRLDNSNAQGEYYLTDVIAMAVADGLDVGAAHPAHPIEVEGVNNRLQLAALERAFQGRLADTLMRDAGVTLADPARFDLRGVLECGRDVFIDAGCVFEGRVELADGVRVGPHCVVKDARIGAGTHIEAFSHIQDAVVGEGARIGPYARLRPGADIGDQAHVGNFVEIKKSRLGKGAKANHLAYIGDARIGARVNIGAGTITCNYDGVNKFQTIIEDDAFIGSDSQLVAPVTVGRGATLGAGTTLTKDAPAGQLTISRTVQRTLEGWKRPVKKP",
    "response#3": "MTILLAAGLGTRMNSDMPKVLHQVAGAPLLIHAMKSAAALTPERTIVVAGHGADAVEAAAKDWDEDVRVALQTEQLGTGHAVLQAKEALAGHTGDAIVLYGDTPFIRPETLEAMIEARQSHAVVVDADGQRLARIVEERDASDAERAIGEVNAGVYAFDAAVLREELARLTTDNAQGEEYLTDVVEIARRDGRRVAAVVADDPWVTEGVNDRVQLATLGAELNRRIVEKHMRAGVTLADPARFDLRGVLECGRDVFIDAGCVFEGRVELADGVRVGPHCVVKDARIGAGTHIEAFSHIQDAVVGEGARIGPYARLRPGADIGDQAHVGNFVEIKKSRLGKGAKANHLAYIGDARIGARVNIGAGTITCNYDGVNKFQTIIEDDAFIGSDSQLVAPVTVGRGATLGAGTTLTKDAPAGQLTISRTVESSIEGWVQRARPGTPAAEAAERARAAHAAEQEGGE"
  },
  {
    "reference": "MPLLDSFKVDHTKMPAPAVRLAKTMTTPKGDEIAVFDLRFCRPNQEILSEKGIHTLEHLFAGFMREHLNGEGIEIIDISPMGCRTGFYMSLIGVPKNARVLEAWRRSMEDILTLKSEEEIPELNIYQCGTACMHSLKEAQEIARTVLDRGISIMDNEALKLQL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must possess iron ion binding to support quorum sensing under certain conditions.2. For general function, the protein need meet that The regulation of gene expression in response to changes in cell density is called quorum sensing; Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD). The designed protein sequence is ",
    "response#1": "ECCFF",
    "response#2": "LVVL",
    "response#3": "LQTN"
  },
  {
    "reference": "MGNIVVVGSVNMDYTAVVNKLPVVGETLLAHDFKLSGGGKGANQAMAAAIFSDNVKMIGMVGDDIIGHALVEKMQEKGIDVSCVEFCEIPTGTALINVDHSGRNTIVVYPGANQQLTPQIIEKYRDVIESADVVMMQLEIPLETVEAVAEIAHQAKVDVLLNPAPAQPLSRELLSKITYLTPNETELLRLASSLETHKGAQILRDQGVKNVVVTLGDKGCYVKTDSMDQRVESYRVNAIDTTAAGDSFSGALAANLAKGHCLMSSIKIANATGALTTTKVGAQEALPTKAEVSELIGRK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should contain one or more Carbohydrate kinase PfkB domains that are essential for its function.2. The protein with the reaction ATP + D-tagatofuranose 6-phosphate = ADP + D-tagatofuranose   1,6-bisphosphate + H(+), the Proton acceptor must be optimized to ensure efficient catalysis.3. Ideal protein should have a binding affinity for key enzymes in the Carbohydrate metabolism, D-tagatose 6-phosphate degradation, D-glyceraldehyde 3-phosphate and glycerone phosphate from D-tagatose 6- phosphate: step 1/2 pathway to enhance the overall efficiency of the pathway.4. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.5. The K(+), substrate, ATP binding site should be stable and able to withstand changes in environmental conditions.6. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium; The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway..7. The ideal protein would be one that plays a critical role in D-ribose catabolic process, D-tagatose 6-phosphate catabolic process, lactose metabolic process through metal ion binding, ATP binding, ribokinase activity within cytoplasm. The designed protein sequence is ",
    "response#1": "MKKILVLGSLNMDFVIRVKKMPEVGETILGEDVALVPGGKGANQAYAAGKLGGHVTMLGAVGMDPYGSSLTKNLQDAGVDTEGILRSKNDATGQAFITVYENGDNSIIVIPGANGRLKPEMLQESMKYIDACDYIIMQLEIPLETVRYVKELALRRGKKVVIDPAPAAAGLEDSFWHGITILKPNETELGILTGKQLRNETEMIEAARSLVFKGVETVLVTLGEKGCLMVREKEAVHFPAKKAEMVDTTAAGDSFLAALVTALCEDRPFEDAVEFAQTVSSIVVGRHGAQTSIPELLEVC",
    "response#2": "MKKILVLGSLNMDFVIRVKKMPEVGETILGEDVALVPGGKGANQAYAAGKLGGHVTMLGAVGMDPYGSSLTKNLQDAGVDTEGILRSKNDATGQAFITVYENGDNSIIVIPGANGRLKPEMLQESMKYIDACDYIIMQLEIPLETVRYVKELALRRGKKVVIDPAPAAAGLEDSFWHGITILKPNETELGILTGKQLRNETEMIEAARSLVFKGVETVLVTLGEKGCLMVREKEAVHFPAKKAEMVDTTAAGDSFLAALVTALCEDRPFEDAVEFAQTVSSIVVGRHGAQTSIPEIQTWIDKESKKKLSMKILVVGSSNVDINVLTHKIPKIGETLMANSMRYSYGGKGANQALTCAKLEADVTFLGCVGDDDHGKAIQENFRSVGINCDRMAISKDEPTGTALITIDETGQNTIVVVPGANFDCDIEYLKANDDAFKTADYILLQMEIPLDTIEYAISRAHHFGKKVILNPAPALPDLNPEIYKKIDYFTPNETELDIMSKSLGIFDSEDDKINALLNAGIKNIIITLGEKGAYFVNRKTRKLIDAIEVNAIDTVGAGDCFNGAFVSALAQGKNEVEAIKFANQAASIAVTREGAQEAIYN",
    "response#3": "MKKILVLGSLNMDFVIRVKKMPEVGETILGEDVALVPGGKGANQAYAAGKLGGHVTMLGAVGMDPYGSSLTKNLQDAGVDTEGILRSKNDATGQAFITVYENGDNSIIVIPGANGRLKPEMLQESMKYIDACDYIIMQLEIPLETVRYVKELALRRGKKVVIDPAPAAAGLEDSFWHGITILKPNETELGILTGKQLRNETEMIEAARSLVFKGVETVLVTLGEKGCLMVREKEAVHFPAKKAEMVDTTAAGDSFLAALVTALCEDRPFEDAVEFAQTVSSIVVGRHGAQTSIPSAEELQGETIGNV"
  },
  {
    "reference": "MRLSEVLPADIAPPPGADAISVSGITAASAAVGPGAIFAGLPGTKVDGSSFVPEAIERGAVAVIVGRNKGVRVPEGVALIEVDNPRAALAKIAAKLAGRQPKTAVAVTGTSGKTSVADFTRQIFTALGHKAASVGTIGIVKPSGAVYGSLTTPDPVTLHAALAALADEGVTHLAFEASSHGLDQHRLDGVELSAAAFTNLGRDHMDYHPTVEDYLSAKLRLFTELLQPGQPSVINADGAYSRDVAAAAKTRGLRVMMVGTGGDALQRTSLSRDGFRQIIGVNAGGKSYEVYLPLVGAYQVENALVAAGLAIAVGEETEAVLQTLRGLKGVPGRLEIIGERNGGLAIVDYAHKPEALAAALDACRPFATGRLISVFGCGGDRDKGKRPIMGRISVERADITIVTDDNPRSEQPAEIRKEILAGAVGAREIGDRAEAITTAVCMMTPGDVVLVAGKGHETGQIIGDRIIPFSDHDEVRKALASR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.2. The designed protein should have a binding site that is specific to the target UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate ligand.3. The designed protein should contain at least one Meso-diaminopimelate recognition motif motif to achieve its intended function.4. The designed protein should contain one or more Mur ligase C-terminal domains that are essential for its function.5. The protein design should consider the endogenous proteins and enzymes present in the peptidoglycan biosynthesis, Cell wall biogenesis pathway to avoid interference with native metabolic processes.6. A protein that can perform UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, magnesium ion binding in cytoplasm to support cell wall organization, regulation of cell shape, cell division. The designed protein sequence is ",
    "response#1": "MKLRDLVHALPYARVEGDLGITADSRRIEPGHVFAAWPGFKTDGRKYIDAAVGQGASAVLWDDVDGYRPDAVAVPLLEIKSISYDSRRTEKGALFFAAHGTEQNGEAFAADAVKKGASAIVAEKFLGSGVPEIKVENVKEAMARLAEAFYGHPSRSLEMIGVTGTNGKTTTTYLIERILSDAGKPAGYLGTLYYHYGRRKLPAPLTTPESVDLHRLLREMVEEGVGAVVMEVSSQGLMLHRVDGLVFEVGVFTNLEPDHIGPNEHKDFEDYKRCKGLLFRQCRLGIANVDDPLAAEMVESSTCPVLTYGIDPGADVRAGDMRLRPGGLGLEVRTPRGRFTLRMPLIGRHNVYNALVAAGAAFALGIPLEAVRPGLEAVRGVPGRLEHVHDPAGRITALVDYAHTPDALRNVLETLRALLPDGHRLSCVVGAGGDRDRSKRPEMARIAAELADQVILTTDNPRSESPEAILDEMEAGVPPAAAPRVLRITDRRQAIRTAVRLGHEVLLVAGKGHEDYQIIGREILPFDDRIVAREAIRGRRG",
    "response#2": "MIRGLVVDSRRLQPGDAFAAYPGERMDGRKFIAQAVAAGANAVLWEREGFEWNDEWEVPNLPVDGLSDKIGAIASQVYAGDPSQRLTMLGVTGTNGKTTTTYLIERILSDAGQSAGVIGTIETRYAGNTLPMSGTTPGALELQQYLAAMAEAGVDSCAMEVSSHALDQGRTWGCRFRIGVFTNLTQDHLDYHGTMEKYAAAKSLLFSRLGNTYSEAEAERSYAVLNADDSASDLFANAAAVEILTYGIDREADVRASDIRITSRGTTFRLETFRGGADISIRMVGKFNVYNALAATSCGLALGMPLDVLEQGITSLGSLPGRMELVGKTKTGASVIVDYAHKPLALETVLKSLRAHVQGQIIVIFGCGGNRDQAKRKMMGEIAKDYADSIIVTDDNPRFEDPQAIRKQILEGVPEAYEIASREEAIAFALHKAEAGDICLIAGKGHEDYQIIGHEKYHFDDREVARDELQRRR",
    "response#3": "MRLQELLAPFGADSVEGNADIEIAGITCDSRRVAEGWVFVCIKGANVDGHRFAAQAEKAGAAVIVAEKDGDFEGVRTVVVPDVRRALAETAARFHGHPSKKLFVVGITGTDGKTTTSFLLRSVLQSAGYRTGLIGTVITSSGEEAVPSLYTTPESLELQEILAHMSLEGNEYVVMEVSSHALALDRVFGIPFEMGIFTNLSRDHLDFHGDFQHYRDVKALLFERLRGQDSCAVINLDDPNAQFIRDKTKVPVVTFSVEMEADVFLLNANLTMEMTEIEASTPQGPIHFQLHLLGRYNVSNALASVARGLVVDSRRLQPGDAFAAYPGERMDGRKFIAQAVAAGANAVLWEREGFEWNDEWEVPNLPVDGLSDKIGAIASQVLGLETGAILRGLAQARVPGRLEHVHDPAGRITALVDYAHTPDALRNVLETLRALLPDGHRLSCVVGAGGDRDRSKRPEMARIAAELADQVILTTDNPRSESPEAILDEMEAGVPPAAAPRVLRITDRRQAIRTAVRLGHEVLLVAGKGHENYQEIRGVKHPFDDRAVAREVLSGR"
  },
  {
    "reference": "MADERSGKGEDSGKLLYCSFCGKSQHEVRKLIAGPSVFICDECVDLCNDIIREEIQEATTSDSSDRLPTPHEIKETLDDYVIGQTRAKLALAVAVYNHYKRLRYEDKKGEVELGKSNILLIGPTGSGKTLLAETLARLLNVPFTIADATTLTEAGYVGEDVENIIQKLLQKCDYDVEKAQKGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVASVPPQGGRKHPQQEFLQVDTSNILFICGGAFAGLEKIIRDRAEKGSIGFSAVVKSQLSSKSVGETLKDVETEDLIKFGLIPEFVGRLPVIATLDELDEAALIQILTQPRNALTKQYSKLFEMESVEVDFREDALRSIAKKAMERKTGARGLRSILEQVLLKTMYDIPSEQDVCKVVIDEGVIAGESEPIKIYKNSEQAKLAADE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects.2. The protein contains novel ClpX-type ZB domains that confer a unique function or activity.3. The protein must exhibit the following characteristics: It directs the protease to specific substrates; ATP-dependent specificity component of the Clp protease; Can perform chaperone functions in the absence of ClpP..4. The designed protein must be optimized for ATP binding, ATP-dependent protein folding chaperone, zinc ion binding. The designed protein sequence is ",
    "response#1": "KSNILLIGPTGSGKTLLAETLARLLDVPFTIADATTLTEAGYVGEDVENIIQKILQKCDYDVEKAEQGIVYIDEIDKISRKADNPSITRDVSGEGVQQALLKLIEGTVASVPPQGGRKHPQQEFLQVNTANILFICGGAFAGLEKIIRSRSEQGGIGFAADVKSKDDSRNVGEVLADVEAEDLIRYGLIPEFVGRLPG",
    "response#2": "QKSNILMLGPTGSGKTLLAQTLAKIINVPFAIADATSLTEAGYVGEDVENILLKLIQAADYDIERAEKGIIYIDEVDKIARKSENPSITRDVSGEGVQQALLKILEGTVASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIDSIIQKRTSKKSMGFGAEIESKQVLDLGSLLKQIQPEDLLKFGLIPEFVGRLPG",
    "response#3": "GKSNIMLIGPTGSGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVENIILKLLQAADYNVERAQRGIVYIDEVDKISRKSDNPSITRDVSGEGVQQALLKIMEGTVASVPPQGGRKHPQQEFLQVDTTNILFICGGAFAGLEKIIGSRGKGGGIGYGAEVRDPDERRTGEILREVEPEDLLRFGLIPEFIGRLPVVATLEDLDEAALIEILTKPKNAL"
  },
  {
    "reference": "MGRNPLIIGITGRIASGKDAASKIISNKYGFYEISADKLGHLVLHEKKEELVKIFGQKILNTRNEIDRLLIRNLVFNDNKELKKLESISHPIIFNKIKKILIQNQSTKIIINAALLFKINLEKLCDYIIVVKAKAPIIKNRLSYSMPSIDSNIINKILEIQKDIFFKKNIINLKIINIINNKNYAYLEKEIEKKMQEIINYERFE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should contain one or more DPCK domains that are essential for its function.2. The protein design must be able to perform ATP binding in order to contribute to phosphorylation, coenzyme A biosynthetic process.3. The protein design should consider the endogenous proteins and enzymes present in the coenzyme A biosynthesis, Cofactor biosynthesis pathway to avoid interference with native metabolic processes.4. The protein should be designed to catalyze the following reaction 3'-dephospho-CoA + ATP = ADP + CoA + H(+). The designed protein sequence is ",
    "response#1": "MXFXGXF",
    "response#2": "MFKLNFLGK",
    "response#3": "MPIGVPKVPFRQPGESDASWVDIYKRLYQERLLFLGQDVDSEISNQLIGLMVYLSIEDDTKDLYFFINSPGGWVLPGIAIYDTMQFVPPDVYSLGMGLAASIATVILVGGEITKRLSMPHARVMIHEPYSNYSGMASDFEIQAKEVLRLRELTSQIYVQRTGQPMWVVSEDMERDVFMSAMEAQEYGIVDIIS"
  },
  {
    "reference": "MTNLSVYQGDDAVLLGSTNEYQDIVGKLAEIDVQLERWHVTVPLEENASAEVVLNSYQQEIEKINQRYGFQAVDVVSLYPDHPQKQELRQKFLLEHTHSDFEIRFFVSGKALFYLHVGNLVYLVLCEAGDLISVPAETTHWFDMGESPNFKSIRFFTTTEGWIADFTGSSISQRFPTFEEYVGSLK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that is capable of catalyzing reactions within the L-methionine biosynthesis via salvage pathway pathway with high efficiency and specificity.2. Ni(2+) is required for the proper folding and stability of the protein.3. The protein must exhibit the following characteristics: Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway..4. The Ni(2+), Fe(2+) binding site should be located in a region of the protein that is accessible to the ligand.5. The protein design must be able to perform nickel cation binding, iron ion binding in order to contribute to L-methionine salvage from S-adenosylmethionine. The designed protein sequence is ",
    "response#1": "MSLLSFFLNKK",
    "response#2": "GRVXVXG",
    "response#3": "MLKRERL"
  },
  {
    "reference": "MTDRLYSSKQIRAIEQQAIAELAQGDFALMQQAASALLGLLKQRWPSRKSLLVYAGNGNNGGDGYLLAAYAKTDGYQVAIKCTGDHNRLPKAALMARQVAEQAQVPLLAPDAPFKADVLVDALFGIGLNRPVAGDYASAIEQINRSAGPVLAVDVPSGLCADTGQVLGSAVKASVTLSFIGHKIGLFTADGPDHCGDLRLEPLSIPKHLLAAQEFSARLVSGPQLLSQLKPRLANFHKGQAGHVVILGGDAGMAGAPLLSAEAALRCGAGVVTVLTRKEHVAALVARRPECMVHGVEAQQDLHPFLLKANVIVAGPGLGQQFWGQQLLQQVLAQNKAVLLDADALNLLAHQAPPQVQQLILTPHPGEAARLLQCNNKDVQQNRLEAVKLLQHRYQATVVLKGHGSLIADAHGVQLLNAGNPGMASAGMGDLLSGIIAALWAQGLSASDATALGCWLHATAADQLAASDGQRGLLALDLLTEVRALLNQSNCG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein have enhanced (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.2. The designed protein must be able to perform NADPHX epimerase activity, metal ion binding under specific conditions.3. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. The designed protein sequence is ",
    "response#1": "MRQMDRQAIEHYGIFEDLLMENAGHAVYFMILEVFPVLEGKRFVVFCGLGNNGGDGLVVARKLHSNGARVNVFLLGDPEKYKGSARKNYEIVKGLSLPMKRLTKIGQAAYPLEHCDAVVDAMLGTGLSRPLEGLYRDVVLAINRAGKKVFSVDIPSGINGDTGDVMGVAVRADATATFGLPKLGNLLYPGFEYGGNLYVTHISFPPAIYESHKGENGILVGIAGSVDYYGAPIYMSKGALRMGIDLLFLFSPEYIHNYISSSTHDIILKKSGERYIEFNNELKRIIEDKATAVALGCGIGKNEVSQESSTKIIEESLLVKKPIVIDADALDSVTLFDDLKGFAVITPHRGEFKRIFSIDPDENNVVKMAKKYNLVILLKGSVDIISDGEIVKKNKSFHHPSMTRGGTGDILTGAVGGLLSRGIDTFHSATLASFIVGSAGKMAFEEKSNSYFTSEVIDMIR",
    "response#2": "MQELDRQTIQEHGIPGLELMEHAGHACMEAIFHEFGYTGGKRAVIFAGRGNNGGDGYVIARLLLQSGWQVRVCILAERERIGGDAAINLEQLPPAAVSYCTVPGQLAGLLQEELSQTDVIVDALFGTGLNSEVAGINREAVELINAAGCPILAVDIPSGIHGTSGRVLGCAVRATVTVTFAAAKLGHVLYPGADHTGRLVVADIGIPAELVDENWGKAALAPRRSTAHKNDFGHVLAVAGSEGKAGAATLVVLAALRAGAGLVTLAARPEVLRTALPGVPEAMGVPLPGNGPLGPGDLAPLRAALRGKTAFAMGPGIPRGPQTGQLLGELLAALDPGCATVVDADALNALAEERDAIAERLRKAPVRPVLTPHPGEFSRLTGEEVERVESDRIDAAVRASSRFGACVVLKGARTVVADPEGTSAVCAHGNPGMATAGAGDVLTGITAAVLARRSGGGSTGERARLAVFLHALAGDLAARRTGETGLVASDIAEVGLPRVFRRL",
    "response#3": "MRQMDRQAIEHYGIFEDLLMENAGHAVYFMILEVFPVLEGKRFVVFCGLGNNGGDGLVVARKLHSNGARVNVFLLGDPEKYKGSARKNYEIVKGLSLPMKRLTKIGQAAYPLEHCDAVVDAMLGTGLSRPLEGLYRDVVLAINRAGKKVFSVDIPSGINGDTGDVMGVAVRADATATFGLPKLGNLLYPGFEYGGNLYVTHISFPPAIYESHKGENGILVGIAGSVDYYGAPIYMSKGALRMGIDLLFLFSPEYIHNYISSSTHDIILKKSGERYIEFNNELKRIIEDKATAVALGCGIGKNEVSQESSTKIIEESLLVKKPIVIDADALDSVTLFDDLKGFAVITPHRGEFKRIFSIDPDENNVVKMAKKYNLVILLKGSVDIISDGEIVKKNKSFHHPSMTRGGTGDILTGAVGGLLSRGIDTFHSATLASFIVGSAGKMAFEEKSNSYFTSEVIDMIK"
  },
  {
    "reference": "MTTTDTASETKPFQAEVAELLNLMVHSVYSETDIFLRELISNASDALDKLRYESIATPALMEAGGAPKIQIVPRKAPDTLTVIDNGIGMNRQELIDNLGTIAKSGTKSFLTKLTEAKDGAGLIGQFGVGFYAAFMVADNIVVTSRRAGSGEVWTWSSSGGAGFEIAPASEEAAARVVRGTEIVLHLKPDAAKYLEAYQIERIVSEYSDNIQFPIELVPEEGEPRQINSASALWQRSKSELTEEDYNQAYKQIAGAFDEPAMTLHYRAEGRQSYAVLLFAPATKPFDLFEPERKGRIKLYVRRVFITADADLLPPYLRFLRGVIDSEDLPLNLSREMLQNNPQLAQIRKAVTGKVIGELESLADKKPEDFARIWEAFGPVLKEGLYEDYERREKLLALARFTTTAGEKRTLSQYVEAMKENQTEIYYLVGDSIERLKSNPKLESATARGIEVLLLTDGVDAFWTSGQLDFGGKPLKSLSQGDVNFDLIPKLDADKPDDKPDETKADEATVIAVIKDALGERVSDVKASQRLTSSASCLVAGGFGPDRELEKMLARANKGAATKPVLEINLGHPLVAALADAKADKADATDLSFLLLEQAQILDGELPEDPAAFAGRLNRLVLRGLVAHG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must be able to ATP binding.2. For general function, the protein need meet that Has ATPase activity. The designed protein sequence is ",
    "response#1": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#2": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#3": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYV"
  },
  {
    "reference": "MASQTQGIQQLLAAEKKAAEKVAEARKRKARRLKQAKDEATEEIEKFRQERERAFKEFEAKHMGSREGVAAKIDADIRVKLADMDRAIQTRKDPFILEILQYVYNISPEVHKNYNHK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Likely acts in maintaining enterocyte lysosomal acidification which consequently promotes Tor activation at the lysosome membrane. The designed protein sequence is ",
    "response#1": "TKKWQSGLISNFEYLMHLNTLAGRSFNDLTQYPIFPFILRDYESEVLDLENPNTFRDFTKPMGAQDPKRLEKFIEKYNDLLEMNEKPYHYGSHYSNIGSVLHFLVRLQPFTSYFIDFQGGRFDVPDRAFHSIAQSWNLSSSISNSDVKELIPEFFYLSDFLVNSNKFFMGIKQNGVKVDDVILPPWAHNDPRLFIKKHNEALECKYVSENLHHWIDLLFGYKQQGEAAVKAHNMFFPLTYEGAVDIDSIEDQLNRDAAVAQIHSYGQTPKQIFTKPHPKR",
    "response#2": "TKKWQSGLISNFEYLMHLNTLAGRSFNDLTQYPIFPFILRDYESEVLDLENPNTFRDFTKPMGAQDPKRLEKFIEKYNDLLEMNEKPYHYGSHYSNIGSVLHFLVRLQPFTSYFIDFQGGRFDVPDRAFHSIAQSWNLSSSISNSDVKELIPEFFYLSDFLVNSNKFFMGIKQNGVKVDDVILPPWAHNDPRLFIKKHNEALECKYVSENLHHWIDLLFGYKQQGEAAVKAHNMFFPLTYEGAVDIDSIEDQLNRDAAVAQIHSYGQTPKQIFTKPHPKR",
    "response#3": "TKKWQSGLISNFEYLMHLNTLAGRSFNDLTQYPIFPFILRDYESEVLDLENPNTFRDFTKPMGAQDPKRLEKFIEKYNDLLEMNEKPYHYGSHYSNIGSVLHFLVRLQPFTSYFIDFQGGRFDVPDRAFHSIAQSWNLSSSISNSDVKELIPEFFYLSDFLVNSNKFFMGIKQNGVKVDDVILPPWAHNDPRLFIKKHNEALECKYVSENLHHWIDLLFGYKQQGEAAVKAHNMFFPLTYEGAVDIDSIEDQLNRDAAVAQIHSYGQTPKQIFTKPHPKC"
  },
  {
    "reference": "MRSGECDEEEIQAKQERDQNQNHQVNLNHMLQQQQPSSVSSSRQWTSAFRNPRIVRVSRTFGGKDRHSKVCTVRGLRDRRIRLSVPTAIQLYDLQDRLGLSQPSKVIDWLLEAAKDDVDKLPPLQFPHGFNQMYPNLIFGNSGFGESPSSTTSTTFPGTNLGFLENWDLGGSSRTRARLTDTTTTQRESFDLDKGKWIKNDENSNQDHQGFNTNHQQQFPLTNPYNNTSAYYNLGHLQQSLDQSGNNVTVAISNVAANNNNNLNLHPPSSSAGDGSQLFFGPTPPAMSSLFPTYPSFLGASHHHHVVDGAGHLQLFSSNSNTASQQHMMPGNTSLIRPFHHLMSSNHDTDHHSSDNESDS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: miR164); Participates in ovule develpment.; Plays a pivotal role in the control of morphogenesis of shoot organs by negatively regulating the expression of boundary-specific genes such as CUC genes, probably through the induction of miRNA (e.g.2. A protein that can perform DNA-binding transcription factor activity, sequence-specific DNA binding in nucleus to support leaf senescence, response to heat, leaf development, regulation of DNA-templated transcription, leaf morphogenesis.3. The protein must contain a signal peptide for proper functionality.4. The designed protein have TCP domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MSGVNVGGDYVEVMCGCTSHRYGDAVARLRVFPTGDLEITCECTPGCDEDKLTPAAFEKHSGRETARKWKNNVWVIIGGEKVPLSKTVLLKYR",
    "response#2": "AAMFDMEYARWLDDDTKRLAELRGGLQAHLLDGNLGLIVEECMQHYDELFQLKAALARSDVFHLLTGSWATPAERCFFWMGGFRPSELLKILIPQLDPLTEQQLLGICNLQQSSEQAEEALAQGLHQLHQSLADTVAAGTLNDGAAAPNYMNIMAVALEKLASLENFYQQADNLRHQTLHQMRRILTTRQAARCFLSIGEYYSRLRALSNLWASL",
    "response#3": "QGFSQLIFGSKYLHSVQEILSHFAAYSLDYSSRGTESGAASSAFTSRFENITEFLDGDSNNSEAGFGSTFQRRALEAKKTHLLDLLQMVDDRYSHCVDEIHTVISAFHAATELDPQLHTRFALQTVSFLYKNLRERICG"
  },
  {
    "reference": "MGGKWSKGWPAVREGMRRAAAPAAEGVRRAAPAAEGVGAASRDLDRHGALTSSNTAATNADCAWLEAQEEGEEVGFPVRPQVPLRPMTYKAAFDLGFFLKEKGGLDGLIYSKKRQEILDLWVYHTQGFFPDWHNYTPGPGVRYPLTFGWPFKLVPVDPKEVEEANEGEDNCLLHPMSQHGMEDEDREVLKWQFDSSLARRHMARELHPEYYKDC",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike2. The protein's functional design necessitates the inclusion of a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Dileucine internalization motif; necessary for CD4 internalization, Diacidic; necessary for CD4 internalization motif.3. The designed protein should have GTP binding, SH3 domain binding within membrane, host cell plasma membrane, extracellular region, host cell Golgi membrane, virion component to regulate suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host autophagy. The designed protein sequence is ",
    "response#1": "MGGKWSKRSIVGWPAVRERMKRAEPAAEGVGAVSRDLEKHGAITSSNTPANNAACAWLEAQEEGEVGFPVRPQVPLRPMTFKGALDLSHFLKEKGGLEGLIYSQKRQEILDLWVYNTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPDKVEEANEGENNSLLHPMSLHGMEDEDREVLMWKFDSRLAFHHVARELHPEYYKNC",
    "response#2": "MGGKWSKSSIVGWPEVRERIRRTPPAAEGVGAASQDLDKHGAITSSNVNHPSCVWLEAQEDEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLVWSQKRQDILDLWVYNTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPDKVEEANEGENNRLLHPISLHGMEDPEKEVLMWKFDSRLAFHHVARELHPEYYKNC",
    "response#3": "MGGKWSKLSGWSTVRERMRRAEPAAEPAAVGVGAVSRDLDKHGAITSSNMNNADSVWLQAQEEEEEVGFPVRPQVPVRPMTYKAAVDLSHFLKEKGGLEGLIYSQKRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEQEKVEEETEGENNSLLHPMSLHGMEDPEKEVLEWRFDSRLAFHHMARELHPEYYKDC"
  },
  {
    "reference": "MEDQTTDHTDTLTTAAFDAATVRALEQPLLADEVPLMRMAANATARTILDVIDQEEWDEPSLRVCVLAGAGDNGGDGLYTGAILAAEGLNVTAIAVGRTLHDAAYEAFLDAGGHIYALDPSNDIPGCPSGFPAGEAGQRLEHAIAFAGKAHILIDAMTGIGGTGALHGIPAAIASVLGRTVTPPRRLGLPNDELAIDFPFVVAVDTPSGIGVDDGSLPGAYIPADVTMMFGALKPCAMLPPASLACGNVVLVDFGFDVDDATPTVETVDCGYAQNALRIPLVEDAKYNRGVVGLITGSTQYPGAAVLSSCAAARSNTGMIRYLGPERAQNLVLQSLPEAVMGKGHVQAWVTGCGVPDTDHADTTDIQRTAISALLAHYSLTQDGANSEALAMPPIVVDAGALDLLPDHVPPQVVITPHAGELARLLTRIDAPTTREEVMAAPLRKAQRAYELTGATVVLKGAYTVIVGTDGNGGMRTLVCGRAPAFLATAGAGDVLAGIIGALLAQRAQELIDDPTLVAEMAAGAVYTHGLAAAMAAHSDQHAWQTPHLYGEPKPDIAQSACGHPIIASDVIAALPSAFDLLNTTARYED",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for (6S)-NADPHX binding.2. The protein should have a fast turnover rate for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.3. The designed protein should be optimized for NADPHX epimerase activity, NADHX epimerase activity, kinase activity activity in order to improve phosphorylation.4. The protein requires Mg(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "DVARMLPVPAAESDKYRRGVVGIAAGSARYPGAAVLAVAGALRGGAGAVRYVGPAGQAVIARFPETLVSDGGPKHAGRVQSWVVGPGAGDDAATVAEVLAAEVPVLIDADGLRLAEASAVRARRAPTVMTPHAGEAAALLGVEREEVEGGRLAAVRELAGRYGATVLLKGSTTLIASAGGAAVRVNPTGTSWLATAGSGDVLSGLAGSLLAAGLPAVDAASAAAYLHGLAGRFAADGAPAGAHDVAEEIA",
    "response#2": "GMTGAALLAASAALHGGAGRVMVALLDGGDLSVDASQPECMLRRFDVLPLDQGAVVCGCGGGEAVRAVLDQVLAQARRLVLDADALNAVAGDPSLATALRARAGRGWHTVLTPHPLEAARLAGLKTHDVQADRLAAATALARQFDCTVVLKGSGSIIAAPGRTPRINPTGNARLATGGTGDVLAGLLGARLAAAADMPEAAFDAAVDACWLHGRVADQWPPDSAR",
    "response#3": "VGIVAGSARYPGAAVLAVAGALSGGAGAVRYAGPATDAVIARFPETLVTAGPPAEAGRVQAWVIGPGLGDGSEARQALVDVLSSDVPVLVDADGLRLLDPQAVRARRAETLLTPHAGEAAALLGLSREEVEASRLSAVRALADRFGATALLKGSTTLVADGTRAIRVNPTGTPWLATAGSGDVLAGLTGSLLAAGMNARDAASVGAYLHGLAARALA"
  },
  {
    "reference": "MYLSELLDGLTRLIPTDVTVSAITNDSRKITPQTVFFAQAGLRSHGLDYLRDECLKGKSQPAAVVYEPPYDLSRFACADNWIAIPSLSQHMSEIGRRFYQPIFDIAPIGVTGTNGKTSVTQYIAQLADFSVIGTMGYGRLNGGASDALTALSHTTPDALAVQAILAELSQQSSGVAMEVSSHALALHRVSAVDFGVAVFTNLTQDHLDFHGDMAQYFLEKAKLFMLPTVHTAIINVDGMHDNHGGESDASDASDASDVGNYGARLAAQVQAAGKCVWVYGESDAVLDYDNAVRLTDIQLSANGISVKLQRKTNGKQWTENLTAPVWGRFNAHNLVAAMLALQASGFAWAALLNKTASIRGVTGRIDPIHLPNGGVAVIDYAHTPDALIRTLQSLREQVSGKIIAVFGCGGDRDKGKRPLMAAAVEAHADAGIITDDNPRTESSADIIADIWQGVENEAKFTVVASRKTAICAGLAQLQAGDVLLIAGKGHETYQIIGDQTFDFSDYAVVAEWLVTSQETRKKCAKNAER",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein contains novel Mur ligase central, Mur ligase C-terminal domains that confer a unique function or activity.2. The protein design must feature the Meso-diaminopimelate recognition motif motif.3. The designed protein should be involved in cell division, cell cycle, cell wall organization, regulation of cell shape by localizing to cytoplasm and exhibiting UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, ATP binding, magnesium ion binding.4. The protein should be designed to have high stability and activity towards ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.5. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.6. A protein should be able to manipulate the activity of enzymes in the Cell wall biogenesis, peptidoglycan biosynthesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "MKLAELAGALGLPPTGGASVEISGITHDSRAVRPGDVYAALPGARAHGADFVAQAAGLGAAAILTDPTGAERAAATGLPVLVVENPRGRMGELAAEVWYGNPSARLQLVGVTGTNGKTTTTSLLEYLWSGSGLPAGVIGTLGARYHTQGKTVSLPSARTTPEASELQKNLALMRDSGVQVVAMEVSSHALSLHRVDGIYFELSLFTNLTQDHLDFHHTMEEYFLAKSALFTSKFSAGALVNADDSFGKRLLAQCEIPAISISTDKGDWQLSLTGFQLTRPGGAIHIIDSPLVGDFNRMNAAMALVVTGVTLRSKDVRAGDLFAALPGAASHGGRHVGEALERGAVAVLTDPEGMAHIGADAAVPVLVHPAPRTVLGQLAAAVAGDDPQTAADGVAAVPGVPGRLEQVETGRDFHVVVDYAHKPDAVEAALSTLRPLTAQAGGRLICVIGAGGDRDTGKRPVMGEIAARLADLVIVTDDNPRSEDPAVIRAAVLEGTRDGAGEVREVGDRREAIRVALSSAGPGDIVLVAGKGHEQGQDIAGVVHPFDDREVLREAITALGGHA",
    "response#2": "MKLRELIDHIESLAIDSRLVATGSLFLACSGSARHGLEFLSQALSAGAVAVVCEPDEQWSAEHIEQLAAETQVPLLQVEGLSRQVSRIAGRFYGEPSARLTVVGVTGTNGKTSIVSLLHELFINEGYKSGLISTIKVEYGDKIYDNTHTTPNALLINTYLSDMVGLGITHCFMEVSSHGITQHRIEGLVFKGGVFSNLSHDHLXYHGSFKKYRDTKKEFFDNLPKSAFALTNLDDKNGAFMLQNSKAKKYTYSVKHHADYKAKLLECQFSGMLLKIKSHEVWTSLVGNFNIQNLLAVFAVGECLALGLEPAEIARGVAAFGGVPGRLEQVETGRDFHVVVDYAHKPDAVEAALSTLRPLTAQAGGRLICVIGAGGDRDTGKRPVMGEIAARLADLVIVTDDNPRSEDPAVIRAAVLEGTRDGAGEVREVGDRREAIRVALSSAGPGDIVLVAGKGHETYQIVGRRRFHFDDREEAARALRAQRGG",
    "response#3": "MKSGLSVDSREVREGHLFAALPGSTAHGAGFVAMAIEKGAAAILTDAEGARIAATALDGASVALVLSEEPRQALARAAALFGRPAERLRVIGVTGTNGKTSIVSLLHELFINEGYKSGLISTIKVEYGDKIYDNTHTTPNALLINTYLSDMVGLGITHCFMEVSSHGITQHRIEGLVFKGGVFSNLSHDHLXYHGSFKKYRDTKKEFFDNLPKSAFALTNLDDKNGAFMLQNSKAKKYTYSVKHHADYKAKLLECQFSGMLLKIKSHEVWTSLVGNFNIQNLLAVFAVSSALVTAGIPLDDAVATLERVRGAEGRMEHFGGGEQPLVVVDFAHTPDALEHALVALRPHAKGELSVVFGCGGDRDRGKRAGMGAVAERLADRVWVTDDNPRSEDGDGIIKDILNGVSRPETVVVQRDRAEAIADAINRASPGDLVLVAGKGHETYQVIGDTVLHFDDREEAAAALEARL"
  },
  {
    "reference": "MHMQDAEAQVADYLRQHEHKQLLRFITCGSVDDGKSTLIGRLLHDTRRLLDDQLHALEADSRRHGTQNGAIDFALLVDGLAAEREQGITIDVAYRFFDTERRKFIVADCPGHEQYTRNMATGASTADLAVVLVDARKGLLTQTRRHSYIVSLLGIRHVVLAVNKMDLVGYDPAVFARIEEAYRALAAQLGIARVDAVPLSALRGDNMTARSPAMPWYDGPALLELLETVPIARERGDAPFRMPVQWVCRPDQDFRGFAGTVACGRVAPGDEVAVLPSGRRSRVARIVTAGGDLAQAVAGQSVTLTLADEVDASRGDVIATAAAPAEVADQFAAHLLWMGDAPLLPGRPYLLKLGTRTVGASVTEIKHKIDVNTQDALAAKHLELNEVAVCNLHLDQPVVFAPYAEDRTLGGFILIDRHSNATVAAGTLDFALRRAANIHWQHLDVDKAARARIKHQPPRCVWFTGLSGAGKSTIANLVDRKLLAMGRHAYVLDGDNVRHGLNKDLGFTPEDRVENIRRVAEVARLMTDAGLIVLVSFISPFRAEREMARRLFAPGEFLEVFVDTPLEEAERRDVKGLYAKARRGELRNFTGIDSPYEPPEHPELHLHTHGADPVALADQVVAALGLED",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design must be able to perform ATP binding, adenylylsulfate kinase activity in order to contribute to hydrogen sulfide biosynthetic process, sulfate assimilation, phosphorylation.2. The protein should have a high affinity for GTP, ATP.3. A protein that can modulate the overall flux of metabolites through the hydrogen sulfide biosynthesis, Sulfur metabolism, sulfite from sulfate: step 2/3 pathway, enhancing or suppressing its activity as needed.4. The designed protein must have a Phosphoserine intermediate active site that is highly conserved among related enzymes.5. For general function, the protein need meet that With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway The designed protein sequence is ",
    "response#1": "TMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALENDSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADCPGHEQYTRNMVTGASTADLAVILIDARKGVLVQTRRHSYLCHLLGIRNIVLAVNKMDLIDYDQARYDAIVAEYETFAKGIGIESFTAMPISGFKGDNIRLATAGSVDDGKSTLIGRLLLDTDSLPLDHLDSVTDADGAVDLAALSDGLRAEREQGITIDVAYRFFSTPTRNYILADTPGHERYTRNMFTGASNAHVGVLLVDARAGVLRQTRRHARIASLLGVPHLVAAVNKIDLVDYDESRFRELESELRTLADRLGVADLTVVPVAALDGDNVVQRSDRTPWYDGPTLLGHLEEIDLERFITCGSVDDGKSTLIGRMLYESHMLFDDHLAALEADSKKVGTQDGEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKYIVADTPGHEEYTRNMATGASTADVAIILVDASQGVLTQTRRHSFIVSMVGVKRIILAVNKLDLVDYSEDVFNKIVSDYHNFADEALQLDEITAIPISALKGDNIVGPSENTPWYNGQSIIEYLESVEVSKRSIQFAAHLIWMDEAPLVAGRNYILRIGTQTVAASVTAIKHRIDVNTREHLVAKTLGLNEIGFCHISTALPMAFDPYEINRETGSFIVIDRYTNRTIGAGMVADQFEATLVWMSEQPMLRGRSYLLKIGTRTVTATILPLKYKLNVDTLDHVASETLALNEIGVAELEFDRAIAFDPYEQNHETGGFIVIDRITNSTVGAG",
    "response#2": "TMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALENDSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADCPGHEQYTRNMVTGASTADLAVILIDARKGVLVQTRRHSYLCHLLGIRNIVLAVNKMDLIDYDQARYDAIVAEYETFAKGIGIESFTAMPISGFKGDNIEVANQFSAKLIWMEEERLFPGRSYLLKLATRTVPATVTGLKHKIDVNTLDELAAKTLALNEIAEVNIETGAAIAFDPYSDNRETGAFILIDRETNATAAAGMQVADQFAANIVWFDEKALLPGRSYILRTETDQASATVTELKYRLDINDFAHAAAKSLEMNEVGFCTISTQSPIAFDPFAENRATGAFILIDRLTNATVGAGMVADQFEARLVWFDDHAMIPGRSYVLRTECDETPANITALKYRVDINSFAHEAAKSLDLNEVGIVNVATQAPIAFDAYGENRVTGGFILIDRMTNATVGAGMITAPLVRSSNVRVSELFDARVVWMNDRPLLPGRQYNLKLASSEVPATPEAIHHRIDVNTLEHHPADQLGLNEIGYCRIRASRALAFDAYDSNPATGAFILVDRVSNLTVGAGMRVSELFDARVVWMNDRPLLPGRQYNLKLASSEVPATPEAIHHRIDVNTLEHHPADQLGLNEIGYCRIRASRALAFDAYDSNPATGAFILVDRVSNLTVGAGMIT",
    "response#3": "RFITCGSVDDGKSTLIGRLLYESKMLFDDQLAALEADSRKVGTRGGEIDYALLLDGLAAEREQGITIDVAYRFFSTDRRKFIVADTPGHEQYTRNMITGASTADLAIILVDARKGILQQTRRHSYLVSLIGIRNVVLAVNKMDLVGYSRERFNEISREYGEFARKIGIPAVTAIPISGVNGDNIVERSPKTSWYDGPSLMHHLETVEIRPNDRLVDFRFPVQYVNRPNLDFRGFAGTVASGAVARGDEIVVAKSGKASRVRRIVAHGGDLERAVEGQAVTLVLEDEVEVSRGNLLVHPEQVSGQFAAHLIWMDEAPLVAGRNYILRIGTQTVAASVTAIKHRIDVNTREHLVAKTLGLNEIGFCHISTALPMAFDPYEINRETGSFIVIDRYTNRTIGAGMIHHSLRRAQNVHWQPLDVDRDARGAMKNQRPRLLWLTGLSGSGKSTIANLVEKRLHALGRHTYLLDGDNVRHGLNADLSFTPEDRAENIRRVGEVARLFADAGVIAITAFISPFRADRERVRALFPDGEFIEVHIDTPLEVAEERDVKGLYKKARSGQLPNFTGIDSPYEAPEAPEIRVDTTRTSAEEAAELIVARLLGGE"
  },
  {
    "reference": "MENRWQVLIVWQVDRMRIRTWNSLVKHHMYVSRRASGWFYRHHYECRNPKVSSEVHIPLGKAILIIRTYWGLQTGEREWHLGHGCSIEWRLRRYSTQVDPGLADQLIHMHYFDCFSDSAIRQALLGHIVIPRCDYQAGHPRVRSLQYLALTALIRPRKRRPPLPSVRKLAEDRWNNPQKTRDRRGNHTMNGH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.2. The protein design must feature the HCCH motif motif.3. The protein must exhibit the following characteristics: Seems to play a role in viral morphology by affecting the stability of the viral nucleoprotein core; Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G; Finally, Vif also contributes to the G2 cell cycle arrest observed in HIV infected cells.; Recruits an active E3 ubiquitin ligase complex composed of elongin BC, CUL5, and RBX2 to induce polyubiquitination of APOBEC3G and APOBEC3F; Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism; In turn, they are directed to the 26S proteasome for degradation; Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions.4. The ideal protein would be one that plays a critical role in viral life cycle through RNA binding within host cell cytoplasm, plasma membrane, cytoplasm, virion component, host cell plasma membrane. The designed protein sequence is ",
    "response#1": "MENRWQVMIVWQVDRMRIRTWKSLVKHHMYVSKKAKGWFYRHHYESTHPKISSEVHIPLGDAKLVVTTYWGLHTGERDWHLGQGVSIEWRQGKYSTQVDPNLADQLIHLYYFDCFSESAIRKALLGHIVSPRCEYQAGHNKVGSLQYLALAALIAPKRIKPPLPSVRKLTEDRWNKPQKTRGHRGSHTMNGH",
    "response#2": "MKVKGIQRNSQHLWNWGTMLLGMLMICKAAENWXRXEXERAVGIGAMIFGFLGAAGSTXGRXLTXFLXPTXRGPDRPEGIEEEGGEQDRDRSVRLVSGFLALAWDDLRSLCLFSYHLLRDLILIVTRTVELXQRGTDRXLXATQRGGIXXXRXVXGLXXXLRDX",
    "response#3": "MKVKGIRKNYQHLWRWGTMLLGMLMICSAAEEKXTXLGRVXLGXLTXSPKGXDXLGXXKDNGXKRGXVXFXKDXGXEXWKYXXXNXKXXXTXHXPXXXKXCSXNXXXTXXXAXXXQXIXHXKXNXTXHXXXCXXXXXGXNXXIXNXHXXXXQXXXNXXHXXXXXXXXTXXXXXKXXNXKXXXQXAXPXIXBXXKXXXQXXHXXXHXXXXXXPXXXKXSXXXXXXXXXXIXCXQXTXXXXXXXXXXH"
  },
  {
    "reference": "MSLQADFDQAAQDVRKLKSRPEDEELKELYGLYKQSVIGDINIACPAMLDLKGKAKWEAWNLQKGLSKEDAMCAYISKARELIEKYGI",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Binds medium- and long-chain acyl-CoA esters.2. The protein contains novel ACB domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MELQEKFDAAVEIIQKLPKTGPVATSNDQKLTFYSLFKQASIGDVNTDRPGIFSIIERKKWDSWKELEGVSQDEAKERYIKALNDMFDKIAS",
    "response#2": "CRENNIDHITKAIKSKTVDVNMTDEEGRALLHWACDRGHKELVKVLLQCEAGINCQDNEGQTALHYAAACEFSDIVELLLQSGADPTLRR",
    "response#3": "MGSLAELFEKAAAHVQGLVQVASREQLLYLYARYKQVKVGNCNIPKPNFFDFEGKQKWEAWKALGDSSPSQAMQEYIAAVKKLDPGWNK"
  },
  {
    "reference": "MSVNDLRPGTTFLYDGNIYLVLEQAFSKTGRQQGKVTVKAKNMRTGARVELTFTGGEKVDKAMIERKEMQYLYNDGNDAYLMNTETYEQVSIPMTRLEWEKNFLVDGLMINMTEFENEVLGIDLPVKVELTVVEAEAAVKGDTTSGAQKKAILETGLEIMVPLFVNQGTKIIVSSADGKYVGRA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Involved in peptide bond synthesis2. The protein should have translation elongation factor activity and be active in cytoplasm. The designed protein sequence is ",
    "response#1": "KEDFASIRTGRPSPATFSRITVEYYGAMTPVNQLASFQVPEPRMVIISPYDKAAMPAIEKAIRESDLGVNPSNDGNIIRVVFPELSEERRKEYVKVARNKAEDGRTSVRNVRRQAKDAIDKAVKAGEIGEDEGHRAQKELDALTQKYVGEIDELLKHKESELL",
    "response#2": "VRNIGIVAHIDHGKTTLSDNLIAGAGMMSEELAGKQLVLDYDEQEQARGITINAAVASMVHAFQGKEYLINLIDTPGHVDFGGDVTRAMRAVDGVIVVVDSVEGVMPQTETVIRQALREYVKPVLFINKIDRLINELRLNSDEMQKRFTKIISDVNKLISKYAPKEFTKEWQVSVQDGKVAFGSAYNNWAISVPSMAETKITFKDIVEYVKNGKQKELAQKNQLHKIILNMVIRHLP",
    "response#3": "VRNIGIVAHIDHGKTTLSDNLIAGAGMMSEELAGKQLVLDYDEQEQARGITINAAVASMVHAFQGKEYLINLIDTPGHVDFGGDVTRAMRAVDGVIVVVDSVEGVMPQTETVIRQALREYVKPVLFINKIDRLINELRLNSDEMQKRFTKIISDVNKLISKYAPKEFTKEWQVSVQDGKVAFGSAYNNWAISVPSMAETKITFKDIVEYVKNGKQKELAQKNQLHKIILNMVIRHLP"
  },
  {
    "reference": "MKIACTLVLFVMLRCYVNARNIPGTCRTHTGIILLSGEEWKDPNHCSTYRCSIFDGEAELEGVTCAAYHVPPHCRLVSAANELYPQCCPTVICSDKSR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must be able to toxin activity.2. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "ITQIPLVKAHTLQSGTTVVRGPGFTGGDILRRTSGGPFAYTIVNINGQLPQRYRARIRYASTTNLRIYVTVAGERIFAGQFNKTMDTGDPLTFQSFSYATINTAFTFPMSQSSFTVGADTFSSGNEVYIDRFELIPVI",
    "response#2": "ITQIPLVKAHTLQSGTTVVRGPGFTGGDILRRTSGGPFAYTIVNINGQLPQRYRARIRYASTTNLRIYVTVAGERIFAGQFNKTMDTGDPLTFQSFSYATINTAFTFPMSQSSFTVGADTFSSGNEVYIDRFELIPVN",
    "response#3": "MEKVRLEVQTILNQTLQTDRVATLKAEYEGFIHLGKVFTDYVSQSTFTPATAKTHFLNMSNLLIQRLPQFEIAGYEGVSISLFTQMCTLHLGLLKDGILAGSDWGFTPEDKDSLICQFNRYVNEYNTRMMGLYSIEFGRLLAKNLNEALNFRNMCSLYVFPFSEAWYQ"
  },
  {
    "reference": "MSYTTLTLQSTQQQSILSDTNRLRLFSGSANIPLAQEVARYLGMDLGPMIRKQFADGELYVQIQESIRGADVYLLQPCCNPVNDNLMELMIMIDACRRASARQITAVIPYYGYARADRKTAGRESITAKLVANLITEAGANRILAMDLHSSQIQGYFDIPLDHVYGSPVILDYLASKNLPDLVVVSPDVGGVARARAFAKKLNDAPLAIIDKRRQAHNVAEVMNLIGDVKGKTAVLVDDMIDTAGTLLEGARLLRAEGARHIYACATHAVFSEPAVTRLSSGLFEEVIVTNTIPIPEAKQFEQLTVTSVANLLGETIWRIHEDSSVSSMFR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P)..2. The presence of Ribose-phosphate pyrophosphokinase N-terminal domains is critical for the interaction of the designed protein with its intended targets.3. We require a protein that can specifically bind to the metabolites produced in the 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, Metabolic intermediate biosynthesis pathway.4. The protein should be able to bind Mg(2+) ligand in a variety of conditions5. The protein design must be able to perform kinase activity, ribose phosphate diphosphokinase activity in order to contribute to phosphorylation, ribonucleoside monophosphate biosynthetic process.6. The protein have enhanced ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-   diphosphate + AMP + H(+). The designed protein sequence is ",
    "response#1": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGVDRVLTVDLHASQIQGFFNIPVDNAAPVMYQYLKQMQVGDLVVVSPDVGGLKMAYAYSQVLSAGLAIVAKRRKSATEVESLTVIGEIRGKNVLMVDDLTETAGTLTTAAALLKRKGAKRILACVSHVLLNDLGVERLRKSSIDELITTDSLAVGERVEGRLLPKLSELLRGTPVAVVSPDAGGVERARAYAKRLEAQLAIIDKRRSRANQVEEMRIIGDVSGCAAVIVDDIVDTAGTLCRAAQAVAEAGATRVIGCCAHGVLSGNALEKIAESALDVLFITDTVPLPPEQQIARLTVLSVAPLLARAIREVFDDGSVTSLFDGTA",
    "response#2": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGADRALVVDLHAPAIEGFFDIPVDHLLGALPLLTDYIESKLDTSEVTVVAPDSGRVRVAERWTDRLGCPLAIIHKRRDPDVPNQVKMFDVVGEVHGRTCVIVDDMIDTAGTITKAAESLKEHGAAGVIAAATHGVLSGPAADRLKNSEISEVVLTNTIPLPPNKKFDKITQLSIAPLIGRAVQLISEGGSVSALFKS",
    "response#3": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGADRALTMDLHTAQIQGFFDGPVDHLIPLFAHYYREKKMIGDEYVVVSPKNSGVQRTRSLAEYLDTAIAIVDQDEADGGYVIGDVAGKKCILVDDILNTGKTLSKAADLLKEAGASEIYACVSHALLSPPASPVILDYLLQKELEDFVVVSPDVGGVARARAFAKKLNDAPLAIIDKRRQAHNVAEVMNVIGDVKGKNAILVDDMIDTAGTLLEGARLLKKEGAKKIYACATHAVFSGPAISRLSSDVFEEVIVTNTVPLTEQQRFEQLTVLSVAPLLAEAIIRVHENRSVTELFV"
  },
  {
    "reference": "SDGHQYIDKAVAQGATVVVCENIPSNPDLHITWVQVSDSSKALGRMACNFYDNPSEKIKLVGITGTNGKTTTVTLLYRLFESLGYKAGLISTVVYKVHEQSYEATHTTPDALTINRLLAQMVDQGCSYAFMEVSSHSLVQHRTEGLNFAVAIFSNITHDHLDYHGTFDNYIRAKKMLFDSLPAGSYALINLDDRNSRVMVQNTKAQVKTYAMRTMADFMVKVIESHFDGMHLQINGIDMHTPFIGHFNAYNLLAVYATAVLLGVEPTEALTHLSALHDVPGRFQAIRSANGILAIVDYAHTPDALVNVLNTINQLRKGNEQLITVVGAGGNRDKTKRPVMARVCVEQSDRVILTSDNPRNEDPNDIINDMLAGVDNASRKKVLTITNRAEAIRTATLLAKPGDIILLAGKGHETYQEIQGVKHHFDDREEVKRAFENL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..2. The protein should have a high affinity for meso-2,6-diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "ITGTNGKTSCSNYLAQALEYLGHRTAVIGTLGNGFLDNLQPATHTTPDAIALQGLLAELKRAGAEYVVMEVSSHALDQQRVAGMQFAAAGFTNLTRDHLDYHGDMAQYGDAKARLFVDYTPALQVINLDDVFGRTLWQRLCPEEARVLGFALSEPGADLGVEELEFTAEGCRFVLSAGQERHRVATALMGSFNVSNLLLCAGLALETGTFDRSRIEAALTDFTGVPGRMEQFHADGSPLVVVDYAHTPGALEQALTALRAHTTGRLCVVFGCGGERDTGKRPLMGEVAARLADRVLITDDNPRGEDAAVIRAAVAAGAGEGAEVVAGRGVAIGGAIAWGAPGDVVLIAGKGHETGQIIGDRVLPFSDHDAARQAIARG",
    "response#2": "ITGTNGKGTTAYLLDAVLARAGRRTGVVGTLGVKVGARTTPLDRTTPEAPDLQRILRQMADQHLDCVMMEVASHALALHRVDGCRFRAAVFTNLTQDHLDFHKTLDAYRAAKRTLFEMVDADGNATINVDDPSASAMAASSRAPVVTYGLTSRADVRAERVRLHLGGSEFMAVTPRGRLPVRLRLHGRFNVYNALAAIAACRALGVSDAAVTGTNGKTSTVEMTRQIWRMCGFSAASIGTLGVTTPDESVSTGLTTPDIVTFLSNMTGLAREGVTHVAYEASSHGLSQFRNEGLKVAAGAFTNLSRDHLDYHDTMEEYFAAKMRLFDEVVVDGGKAVIWADDPHSDAAIDHARRRGLSVLSIGNRAGEDGIRLSGRQPTELGQELALGFGGAEYKVRLPLIGAYQAANALMAAGLCIASGVEADAVFAALAETGGVRGRMENISAPGKATAVVDYAHTPDALEKALQACRRHCQGQLWLVFGCGGDRDKGKRPEMGKIGEQLADHVVITNDNPRHEAAELIASDILAGCQKSEKITLILDRAQAVRSVLSRAKAEDMVLLAGKGHETYQIVGTERRHFDDREEAARALAAL",
    "response#3": "ITGTNGKGTTAYLLDAVLARAGRRTGVVGTLGVKVGARTTPLDRTTPEAPDLQRILRQMADQHLDCVMMEVASHALALHRVDGCRFRAAVFTNLTQDHLDFHKTLDAYRAAKRTLFEMVDADGNATINVDDPSASAMAASSRAPVVTYGLTSRADVRAERVRLHLGGSEFMAVTPRGRLPVRLRLHGRFNVYNALAAIAAARQLGVPDVAIAQGLQRLTGVPGRMQLAATRLNGAAVFVDYAHTPDAVATALKAMRPHVMGRLVAIVGAGGDRDPGKRPLMGAAAAQNADIVFVTDDNPRSEDPADIRTMVLEGAPEAIEVGDRAEAILRGVDALGPGDALLIAGKGHEQGQIVGDRVLPFDDVSVARAALAAG"
  },
  {
    "reference": "MRVGIVGATGQVGTVMRRILVERKFPVDELRLFASARSAGKTLDGVTIEDAATADYSGLDIVLFSAGGATSKALAEKVAGQGAVVIDNSSAWRKDPEVPLVVSEVNPHAIANRPKGIIANPNCTTMAAMPVLKPLHEEAGLTALIATTYQAVSGSGLAGVAELHGQVQKVAAEADKLTHDGSAVDFPEPDVYKRPIAFNVLPLAGSIVDDGLNETDEEQKLRNESRKILEIPELKVSGTCVRVPVFSGHSLQVNLRFERPLSPERATEILGSAPGVELSEIPTPLQAAGQDVSYVGRIRSDETAENALSLFLSGDNLRKGAALNAVQIAELVAEELGAK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. Ideal protein should have a binding affinity for key enzymes in the Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5, L-threonine biosynthesis pathway to enhance the overall efficiency of the pathway.2. The protein have enhanced L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.3. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.4. The protein must have high specificity towards NAD binding, NADP binding.5. The designed protein should be able to compete with endogenous proteins or inhibitors for NADP(+) binding.6. The presence of Semialdehyde dehydrogenase NAD-binding domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MSDTVALVGATGAVGRIMRQLLEERDFPAREFRFLASARSAGTTLTFKGKQYTVEELRKDVFAGVDLVISTTPDDTARDFLPAAVEAGATVIDESGYWRMNESVALVIPEVNPQAALTADDKIVANPNCSTIQMVLALRPLHDEAGLKRLVVSTYQAVSGGGLAGIDVLARQLDHVGDQVRALATGGHELLPPAEKWAVPIAHNVVPLNYVLGEDDYTEEELKLRDETRKILEIPGLPVSGTCVRVPVFTGHSLSINAEFDRPISPARALELLARAPGVVISEVPNPLEATGQDPVFVGRVRHDPTVAHGLALFVVGDNLRKGAATNAVQIAEYLAGSGL",
    "response#2": "MGYNVGIVGATGLVGTMIREILVERRFPVACLRLFASARSAGKVLDGVVVEDAETADFGGLDVVLFSAGGSTSRALAPKAAAAGAVVIDNSSAWRSDPRVPLVVAEVNPHALADRPRGIIANPNCTTVALVGATGAVGRIMRQLLEERDFPAREFRFLASARSAGTTLTFKGKQYTVEELRKDVFAGVDLVISTTPDDTARDFLPAAVEAGATVIDESGYWRMNESVALVIPEVNPQAALDKGAALNAVQIAELLLG",
    "response#3": "MNIAVVGATGAVGQVVLSILEQRNFPVGELRLLASKRSAGTRISVMGEELVVDEAMPDSFDGMDIVFFAAGGSTSQALAPEAAKRGAVVVDKSSAWRMDPTVPLVIPEINPDDIREIPKGIVSNPNCSTIQMVAALAPLHRANPIQRVIVDTYQSASGAGAQAVVELMQQTRDVLDHKVPSHSTFPATLAFNALPHVEDFGEDDYTTEEIKMENETRKIMHLPDLPVSATCVRVPVPISHSEAVHIEFANYMSPDEARDVLSEFPGVRVIDDIASKSYPLADYATGKDEIFVGRIRPDKAFHNGLSIWTVGDNLRKGAALNAVQIAELLL"
  },
  {
    "reference": "MHVTAIIPAGGRGRRLGAGVPKQFLALAGRPILQRTVEAFLRCPAVAEVIVVLPPEMVDAPPAYLQASRVKVVAGGDRRQDSVANGFDALPAGADIVLIHDAARPLVDEPTIERAIAAASERGAAIAAVAASDTVKLSESAEAQGERLVDRTLARDAIFLAQTPQAFRRAVLAEAVALGRSGAEATDEAALAERAGHRVQIVEGSRRNIKITTSEDLVVAEALLEGRGGSRLAPTGGWPVMRVGTGYDLHRLVEGRPLILGGVTIPFERGLAGHSDADVLAHAITDAILGAASLGDIGHHFPDTDPRWKGASSVELLAHVVALLRERNLVVVNVDATVIAERPQLGPHREAIAGRLACAMCLDRGAVSVKAKTNEGVDATGRGEAIAAHAVALIEQRPAGSPRTAESGQE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein with metal ion binding, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity functions is required to enable isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway, terpenoid biosynthetic process.2. The protein should be designed to have high stability and activity towards 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-   erythritol 2,4-cyclic diphosphate + CMP.3. The presence of a divalent metal cation is necessary for the protein to function in the desired environment.4. The protein should have a high affinity for 4-CDP-2-C-methyl-D-erythritol 2-phosphate, a divalent metal cation.5. The protein should be optimized for activity in isopentenyl diphosphate biosynthesis via DXP pathway pathway and exhibit minimal cross-reactivity with other metabolic pathways.6. The protein contains novel 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDDRYKGISSIKLLEEVGKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADALKIDVDRVNVKATTEEGLGFTGSGEGISSQAICSAVKLLATKVRVKITTPADLALLSSPMNEAKPPRTGWGYDVHRYLASNETDAKTKSQARPMMLGGIAIPGGPQVIAHSDGDVLLHALTDALLGVACAGDIGQLFPDTDQAFANMPSAVFVTEALRRVRACGYRPAHADLTVVAQVPKLGPHKARMAAQIAQLLELPQACVNVKATTEEGLGFTGEKRGIKAIAVVS",
    "response#2": "MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDDRYKGISSIKLLEEVGKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADALKIDVDRVNVKATTEEGLGFTGSGEGISSQAICSEAAV",
    "response#3": "MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDDRYKGISSIKLLEEVGKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADALKIDVDRVNVKATTEEGLGFTGSGEGISSQAICSLNFSLED"
  },
  {
    "reference": "MATMASTSNCSPVATSPLMMLLFKALQEGGDSEDEARRRREQLNRRPSYRMILKDLETADKVMKKEPEETPPSSVDASPLQFQSVMRPPPTAPPTSAATPNRILPSSNAASPYGSPLGSSILSNQPLVLPFAPINGDFDFSAAIAAASQPKVFPGGPQQNGLGGGGGGGGVPGPSSGIAGMSVQPPTSSTPSQQQSVQSLEGTSGLIGSAMKPMLGIDAVSFPEFGTTDWQSPMLSGGYSSSPSPTMTGGSMRMGGGPLHGEDESNRKRQVRLLKNREAAKECRRKKKEYVKCLENRVSVLENQNKALIEELKTLKELYCRKEKDGM",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should emphasize Polar residues compositional bias.2. Design the protein with a signal peptide incorporated.3. A protein that contributes to regulation of transcription by RNA polymerase II by functioning in nucleus, transcription regulator complex with transcription coactivator binding, DNA-binding transcription factor activity, RNA polymerase II-specific, RNA polymerase II cis-regulatory region sequence-specific DNA binding.4. The protein must exhibit the following characteristics: [Isoform e]: Plays a role in associative long-term memory (LTM) and learning; perhaps required at the time of acquisition and/or the consolidation phase of memory formation..5. The designed protein should contain one or more KID domains that are essential for its function. The designed protein sequence is ",
    "response#1": "LETKELWDRFHDLGTEMIITKTGRRMFPTVRVSFSGPLRQIQPADRYAVLMDIIPMDSKRYRYAYHRSAWLVAGKADPAPPARLYAHPDSPFSCEALRKQVISFEKVKLTNNEMDKNGQIVLNSMHRYQPRIHLVRLSHGQSIPSNPKELQDLDHKTYVFPETVFTAVTAYQNQLITKLKIDSNPFAKGFRV",
    "response#2": "CHLETKELWDRFHDLGTEMIITKTGRRMFPTVRVSFSGPLRQIQPADRYAVLMDIIPMDSKRYRYAYHRSAWLVAGKADPAPPARLYAHPDSPFSCEALRKQVISFEKVKLTNNEMDKNGQIVLNSMHRYQPRIHLVRLSHGQSIPSNPKELQDLDHKTYVFPETVFTAVTAYQNQLITKLKIDSNPFAKGFRR",
    "response#3": "LETKELWDRFHDLGTEMIITKTGRRMFPTVRVSFSGPLRQIQPADRYAVLMDIIPMDSKRYRYAYHRSAWLVAGKADPAPPARLYAHPDSPFSCEALRKQVISFEKVKLTNNEMDKNGQIVLNSMHRYQPRIHLVRLSHGQSIPSNPKELQDLDHKTYVFPETVFTAVTAYQNQLITKLKIDSNPFAKGFRR"
  },
  {
    "reference": "MCSPSTSDVRFITFVNTTYIEAEGLEAIFDLLLKIEVGFLVFSWIEFLYLFYLFIFIRSMHFNLTFLFMNYGGQYFCSMLSRCIIVYQQLGNDPNNDLHNWILVANFARTVCLFIAMYILPIFMIERCLASFFVKNYEKSRKIWVSLMILSIFHPLVFASAIAYIQCWIPVVVHVISFFIVNIIGYIGIHICYSYNIKKHRKFYSPQCISRVTYGLSERFQLAENIKMCKVLKKVQISILFFNIGCCSILLMDHFQVKMMIIYWSYVCFNFFALVYGITVPIILYSALPEWQKETRRLLNLCIGRRNVGEEPKSTFGEQMIYNDHAIESNIYFTQFNKTTH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be optimized for stability and expression in membrane where it will participate in sensory perception of chemical stimulus. The designed protein sequence is ",
    "response#1": "VKTDQEELLAQELEDLNKWGLNIFHVSDYARGRSLSCIMYTIFQERDLLKKFHIPVDTMVTYMLTLEDHYHQDVAYHNSLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQQDNCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLDLYRQTDRIMAEFFQQGDASGRGDGDQPHVRQHTARGESQVGFIDYIVHPLWETWADLVHPDAQEILDTLEDNRDWYYSAIRQR",
    "response#2": "VKTDQEELLAQELEDLNKWGLNIFHVSDYARGRSLSCIMYTIFQERDLLKKFHIPVDTMVTYMLTLEDHYHQDVAYHNSLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQQDNCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLDLYRQTDRIMAEFFQQGDASGRGDGDQPHVRQHTARGESQVGFIDYIVHPLWETWADLVHPDAQEILDTLEDNRDWYYSAIRQR",
    "response#3": "VKTDQEELLAQELEDLNKWGLNIFHVSDYARGRSLSCIMYTIFQERDLLKKFHIPVDTMVTYMLTLEDHYHQDVAYHNSLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQQDNCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLDLYRQTDRIMAEFFQQGDASGRGDGDQPHVRQHTARGESQVGFIDYIVHPLWETWADLVHPDAQEILDTLEDNRDWYYSAIRQR"
  },
  {
    "reference": "MTRYIFITGGVVSSLGKGIASAALGALLQARGYKVRLRKLDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGVSARQSDNITTGRIYQTIIDKERRGDYLGATVQVIPHVTNAIKDFVLSDAGDVDFVLCEIGGTVGDIEGLPFFEAIRQLNQELEPLQAVFVHLTLLPYISTAGEMKTKPTQHSVKELRSIGIQPHILLCRSERAIPEGERRKIGLFCNVKQDSVIEALDSQHIYEVPLDYHQRGLDKEVLRHFGITNAPDPDLTRWKNISAALRAPDGGVEVAVVGKYVSLPDAYKSLIEALHHGGLANQVRVKIKWVSSELFDSEDSDIESELDGAHAIMLPGGFGERGAMGKMRAAKYAREKRIPCFGICYGMQMAVVEAARNLANIEGAGTSEFGAPSEKIVGLMEEWTRGNQKETRTETTDLGGTLRLGTYPAVLKKGSRVAEIYGAEHIEERHRHRYEVNVKYKEKLEESGMLFSGMSPDGKLPEIVEIPNHPWYIGVQYHPELKSRPFEPHPLFSSFIGAAKVQSRLV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must be able to metal ion binding.2. The UTP binding site should be stable and able to withstand changes in environmental conditions.3. The protein should be optimized for activity in Pyrimidine metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates..5. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "MQDAYISHKEAFSHVTGNTGIKVNIKWIDSDDLVRSKEQLEDIDGILIPGGFGYRGVEGKIIAAKYARENHVPYLGICLGFQAAVIELSRDLIGLDNANSTEFEPDTVNPVIDILPEQKNIKDLGGTMRLGAKPVIINKGTLAYDLYKSEKISERHRHRFEVNPAYIKRLENAGAVFSGTDEEGIRMEILELNDRDNYIATQYHAEFQSRPLNPSKVHLYFLEK",
    "response#2": "MQDAYISHKEAFSHVTGNTGIKVNIKWIDSDDLVRSKEQLEDIDGILIPGGFGYRGVEGKIIAAKYARENHVPYLGICLGFQAAVIELSRDLIGLDNANSTEFEPDTVNPVIDILPEQKNIKDLGGTMRLGAKPVIINKGTLAYDLYKSEKISERHRHRFEVNPAYIKRLENAGAVFSGTDEEGIRMEILELNDRDNYIATQYHAEFQSRPLNPSKVHLYFMQK",
    "response#3": "MQDAYISHKEAFSHVTGNTGIKVNIKWIDSDDLVRSKEQLEDIDGILIPGGFGYRGVEGKIIAAKYARENHVPYLGICLGFQAAVIELSRDLIGLDNANSTEFEPDTVNPVIDILPEQKNIKDLGGTMRLGAKPVIINKGTLAYDLYKSEKISERHRHRFEVNPAYIKRLENAGAVFSGTDEEGIRMEILELNDRDNYIATQYHAEFQSRPLNPSKVHLYFLE"
  },
  {
    "reference": "MSEITTLQPSLLWKWFDQICAIPHPSHYEDTLANFIVNWAKEKHFFVERDEVGNVLIRKPATKGMENHTPVVLQAHLDMVPQANEGNPHDFTKDPIQPYIDGEWVKARGTTLGSDNGIGLASTLAVLESNDLAHPPLEVLLTMTEETGMDGAKGLRHNWLQSEILINTDTEEIGEIYIGCAGGINANFEIPVQYETNTFEHSAQITLKGLRGGHSGGDIHTGRANAIKVLARVLAKLSQNQPHFALSEIRGGSIRNAIPREAAAVLAFNGDVKTIESAVENLAVVLKEELAIAEPNFTLFVESAEKAPTVFTAQSTQTVINALNVLPNGVIRNSDVVKNVVESSLSVGVLKTEGNVVKSTILVRSLIESGKYYVTEMLSSLAILANAKVEFSAPYPGWQPVNDSTILDVTRKHYAEVLGEQPVVKVIHAGLECGLLKEHYPNIEMISVGPTIRNAHSPDEKVEIATVQTYWDLLTRVLADIPAK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design should incorporate a binding pocket that specifically recognizes Co(2+) to enhance enzymatic efficiency.2. The designed protein must possess metal ion binding to support proteolysis under certain conditions. The designed protein sequence is ",
    "response#1": "IVGGYECKAYSQPHQVSLNSGYHFCGGSLVNENWVVSAAHCYQSRVEVRLGEHNIQVTEGSEQFISSSRVIRHPNYSSYNIDNDIMLIKLSKPATLNTYVQPVALPTSCAPAGTMCTVSGWGNTMSSTADKNKLQCLNIPILSYSDCNNSYPGMITNAMFCAGYLEGGKDSCQGDSGGPVVCNGELQGVVSWGYGCAEPGNPGVYAKVCIFNDWIKSFI",
    "response#2": "GGPVVLEEIRVDPPKASEVRIKMLCASLCHTDVLCTKGFPIPLFPRIPGHEGVGVIESVGKDAKGLKPGDIVMPLYLGECGQCLNCKTGKTNLCHVYPPSFSGLMNDGTSRMSIARTGESIYHFASCSTWTEYAVADCNYVLKINPKISYPHASFLSCGFTTGFGATWRETQVSKGSSVAVFGIGTVGLGVIKGAQLQGASKIIGVDVNQYKAAKGKVFGMTDFINPKDHPDKSVSELVKELTHGLGVDHCFECTGVPSLLNEALEASKIGIGTVVPIGAGGEASVAINSLILFSGRTLKFTAFGGVRTQSDLPVIIDKCLNKEIQLDELLTHEIHLDNIQEAFEILKKPDCVKILLK",
    "response#3": "IVGGYECKAYSQPHQVSLNSGYHFCGGSLVNENWVVSAAHCYQSRVEVRLGEHNIQVTEGSEQFISSSRVIRHPNYSSYNIDNDIMLIKLSKPATLNTYVQPVALPTSCAPAGTMCTVSGWGNTMSSTADKNKLQCLNIPILSYSDCNNSYPGMITNAMFCAGYLEGGKDSCQGDSGGPVVCNGELQGVVSWGYGCAEPGNPGVYAKVCIFNDWLTSTMR"
  },
  {
    "reference": "MNKSLRFSHKILLAASLIVILAFSLFTLYNDYLQRNAIREDLENYLAEMGASTSTNIRNLFEGRIKLVENLAQNIAQDPANAETLMGQNALISSFLTVYLGKVDGGFSVRPDAKMPDGYDPRTRPWYKDGMNASGATLTEPYIDMTTNKMVIGILSKVSSSVGVVGGDLALDGLVQIINSLNFGGMGYAFLVNDQGKILVHPDKDLVMKSLSDLFPQHTPKLTGELTEVQSDGQTRLLTFSPITGLPSANWYIGLSVDKDKAFSMLSTFRTSAVIATVVAVVIIIGLLGLLIRVLMQPLHTMTRAMEDIAEGEGDLTKRLHIHSHDEFGVLGNAFNRFVERIHSSIREVSSATEQVNEVALRVISASNSSMTNSDEQSNRTNSVAAAINELGAAAQEIAGNAAQASQHASSARLLAEEGQQVVERNIAAMNRLSDLIVTSSAHIETLNSKTVNIGQILEVITSISQQTNLLALNAAIEAARAGEAGRGFAVVADEVRNLAHRTQESAQQVQTMIEELQVGARESVDTMEQSQRHSQDSMQIANQAGERLDSVTVRIGEIDGMNQSVATATEEQTAVVEAINMDINEINMLNQEGVENLQATLRACSDLEQQAGRLKHLVGSFRI",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: McpG is a specific gamma-aminobutyric acid (GABA) chemoreceptor that recognizes GABA over a wide range of environmental conditions; Contributes to attraction to and colonization of plant roots.; Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation.2. The designed protein have Methyl-accepting transducer, Cache, HAMP domains with specific sequence motifs that are crucial for its activity.3. The designed protein should be involved in chemotaxis, signal transduction by localizing to plasma membrane and exhibiting transmembrane signaling receptor activity.4. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "LVCIGVSTGGTEALRDVLECLPAKAPAILIVQHMPQGFTAAFAKRLDSLCEIEVREAQDGDVVVQGCAYIAPGGRHMLLQRTGQRYHIAIKDGPPVSRHRPSADVLFRSAAQYAGPNALGIIMTGMGDDGARGLLEMHKLGATTRAQDEESCVVFGMPKEAIALGGVDKVVPLAQIPREIL",
    "response#2": "VCIGVSTGGTEALRDVLECLPAKAPAILIVQHMPQGFTAAFAKRLDSLCEIEVREAQDGDVVVQGCAYIAPGGRHMLLQRTGQRYHIAIKDGPPVSRHRPSADVLFRSAAQYAGPNALGIIMTGMGDDGARGLLEMHKLGATTRAQDEESCVVFGMPKEAIALGGVDKVVPLAQIPRES",
    "response#3": "LLSSEKLIAIGASTGGTEAIRAVLQPLPPTSPALLITQHMPPGFTRSFAERLNKLCQITVKEAEDGERVLPGHAYIAPGDRHLELARSGANYQVRIHDGPAVNRHRPSVDVLFRSVAQYAGRNAVGVILTGMGNDGAAGLLEMHRAGAYTLAQNEASCVVFGMPREAIAMGGVNDVVELNQISQRMLAQISSS"
  },
  {
    "reference": "MAAQIPESDQIKQFKEFLGTYNKLTETCFLDCVKDFTTREVKPEETTCSEHCLQKYLKMTQRISMRFQEYHIQQNEALAAKAGLLGQPR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that May also be required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane; Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.; Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane2. A protein that contributes to protein targeting to mitochondrion, sensory perception of sound by functioning in mitochondrial intermembrane space protein transporter complex, mitochondrial intermembrane space, mitochondrial inner membrane, TIM22 mitochondrial import inner membrane insertion complex with zinc ion binding, protein homodimerization activity, membrane insertase activity, chaperone binding.3. The protein design must feature the Twin CX3C motif motif.4. helix-strand-helix-strand-helix is the desired arrangement for the engineered protein. The designed protein sequence is ",
    "response#1": "VKQQAAILTAQNMITDXSERCLTKCITXPGSALSXTERQCLQRCMDRFMETYXLASQXLQI",
    "response#2": "MQQQQQLRNLRDFLLVYNRMTELCFQRCVPSLHHRALDAEEEACLHSCAGKLIHSNHRLMAAYGGGD",
    "response#3": "VKQQAAILTAQNMITDXSERCLTKCITXPGSALSXTERQCLQRCMDRFMETYXLASQXLQRL"
  },
  {
    "reference": "MMTPTIPVALFDRLLVEGISPHELVRRKLMCLFNSCSVPGGETLPPLLTRGMPEWHEVNVGDKRVLNWFCRELRAAILRYEPSINMLKVSVKDAHHQTLALSLEAMLQDESEPLRLEIAYSNGRWR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that contributes to cellular response to DNA damage stimulus, cellular response to oxidative stress by functioning in cytoplasm with anti-sigma factor antagonist activity.2. The protein must exhibit the following characteristics: Inhibits RpoS proteolysis by regulating RssB activity, thereby increasing the stability of the sigma stress factor RpoS during oxidative stress. The designed protein sequence is ",
    "response#1": "LRKSVCSDLLTLFNSPHSALPSLLVSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIMLYHR",
    "response#2": "SLRKSVCSDLLTLFNSPHSALPSLLVSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIMLYHR",
    "response#3": "LRKSVCSDLLTLFNSPHSALPSLLVSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIMLYHR"
  },
  {
    "reference": "MGLTGKKILLGIGGGIAAYKVPELVRRLREQNAKVRIIMTDSAKSFITPLSLQVLSGHLVCDDSLNPNRQQKAIEHIELAKWADLIVVVPATADLISRITTGRANDLITITCLASKMPIAIVPAMNQQMYHAIITQENLKHLQQRGMLVWGPATGNQACGDIGFGRMLEPMDILAYIVSWFQPIKNLQHLHIMITAGPTHEALDPIRYISNYSSGKMGFAIAAAAARCGAQVTLISGPVILQTPNGVRRINVDSALEMQAAVMKDIHQQHIFIANAAVADYRAEHIEIKKIKKQINDDNFILRLIKNPDIVADVAALKKNRPFVVGFAVETQNTKENAKQKLLLKNLDLICANDIKQLGKRFNSDTNSIHLLWQEGEKFLPLNNKVYLGQQLIDEIITHYDEKNQHKNY",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A key design requirement for the protein is the optimization of the Proton donor to achieve high catalytic efficiency for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.2. The presence of Mg(2+) is necessary for the protein to function in the desired environment.3. The protein design must be able to perform phosphopantothenoylcysteine decarboxylase activity, FMN binding, metal ion binding in order to contribute to coenzyme A biosynthetic process.4. The designed protein should contain one or more Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains that are essential for its function.5. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A6. A protein that can modulate the overall flux of metabolites through the CoA from (R)- pantothenate: step 3/5 pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MTVIVGITGGIAAYKAVGVVRDLVKRGHDVHVVPTEGALRFVGLPTLEALSRNPVTTSVWDDVAEVRHVALGRRADLVVIAPATADSLARMAHGLASDLLGTTLLATEAPVVVAPAMHPQMWEHPATRANVATLRDRGVHLVGPVVGALTGDDAGIGRMAEPEDIVAAALALLDPADGALAGVRVVVSAGGTREPFDPVRFVGNRSSGRQGIAIAADAVRRGASVTLVAANVEGSLTAGLDATVVPVGSALELAEAVHAAATEADVVVMTAAVADYRPAEVLTEKLKKDAQGERMTLELVRNPDVLADLVAARRTGQIIVGFAAETEPDRDARIELGRAKISRKPADLLVVNHVGWSAGFEREENAIEVLAADGSESLAGVRVVVSAGGTREPFDPVRFVGNRSSGRQGIAIAADAVRRGASVTLVAANVEGSLTAGLDATVVPVGSALELAEAVHAAATEADVVVMTAAVADYRPAEVLTEKLKKDAQGERMTLELVRNPDVLADLVAARRTGQIIVGFAAETEPDRDARIELGRAKISRKPADLLVVNHVGWSAGFEREENAIELLGADGSEQLVARGSKRVVADAVLDVVAELRRR",
    "response#2": "MTVIVGITGGIAAYKAVGVVRDLVKRGHDVHVVPTEGALRFVGLPTLEALSRNPVTTSVWDDVAEVRHVALGRRADLVVIAPATADSLARMAHGLASDLLGTTLLATEAPVVVAPAMHPQMWEHPATRANVATLRDRGVHLVGPVVGALTGDDAGIGRMAEPEDIVAAALAALDDTAATTERLAGVRVVVSAGGTREPFDPVRFVGNRSSGRQGIAIAADAVRRGASVTLVAANVEGSLTAGLDATVVPVGSALELAEAVHAAATEADVVVMTAAVADYRPAEVLTEKLKKDAQGERMTLELVRNPDVLADLVAARRTGQIIVGFAAETEPDRDARIELGRAKISRKPADLLVVNHVGWSAGFEREENAIEVLDAAGRPVELAGVRVVVSAGGTREPFDPVRFVGNRSSGRQGIAIAADAVRRGASVTLVAANVEGSLTAGLDATVVPVGSALELAEAVHAAATEADVVVMTAAVADYRPAEVLTEKLKKDAQGERMTLELVRNPDVLADLVAARRTGQIIVGFAAETEPDRDARIELGRAKISRKPADLLVVNHVGWSAGFEREENAIEVLGADGSVVAEASGSKGAVAAAVLDRLLAVAPAAGR",
    "response#3": "MTVIVGITGGIAAYKAVGVVRDLVKRGHDVHVVPTEGALRFVGLPTLEALSRNPVTTSVWDDVAEVRHVALGRRADLVVIAPATADSLARMAHGLASDLLGTTLLATEAPVVVAPAMHPQMWEHPATRANVATLRDRGVHLVGPVVGALTGDDAGIGRMAEPEDIVAAALALLARGDQPALEGKQIVITAGPTREAVDPVRYFTNYSSGKMGFALAEQAAKRGAEVTLIAGPVSLPTPENVKRIDVVTADDMFQAVIKAADLADVVIKAAAVADYRPALVSSEKVKKSDDAIAIEMERTKDILWTLGQMKTNQILVGFAAESERLDEYAQKKLKKKNLDLICANNIKAEGAGFDKDTNVMTVFWPGGREELPLQSKQELAHRLLSLVAQRYAAGEAPGA"
  },
  {
    "reference": "MADDKTTHFGYKTVDSKDKVKMVAGVFHSVAAKYDIMNDLMSGGVHRLWKRFTIELSGVRPGNKVLDIAGGTGDLAAKFSRLVGSSGKVVLADINDSMLTVGREKLTNKGIVGNIEYVQANAECLPFPDNYFDCITIAFGLRNVTDKDAALRSMNRVLKPGGRLLVLEFSKPVNPVIAKLYDRYSFDVLPKMGQLVTNDADSYRYLAESIRMHPDQDTLKGMMSDAGFVDVRYHNMTAGVVAVHRGIKP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2)..2. The designed protein should interact with enzymes and substrates within the menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2, menaquinone biosynthesis pathway to enhance or regulate its activity.3. The designed protein must possess S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity to support ubiquinone biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "MAKDIVLATSNVHKARELAEMLPHDVTVLGLDAFPGVPEVVEDGATFEANARIKAEAVAQATGKPSLADDSGLCVDALGGAPGIYSARYAGEPKSDERNNAKLLHELAMVTDAERTAQFRCVMVYVRHADDPQPIIVEGSWEGRILHEPTGTGGFGYDPLFYLPELNCSAAELTSDEKNRISHRGQALRKLLPVLRDLAG",
    "response#2": "MSKLPLVFASNNKNKLREISAMLSNELSLVSLDDIGCHDDIPETADTIEGNAILKANYVSKNYGYNCFADDTGLEVEALNGEPGVYSARYAGEQRNADDNMNKLLEKLRDKPDRSAQFKTVIALQLNGEEHFFEGIVNGTIVKELRGTDGFGYDPVFMPDGYDQTFAEMSLEEKNSISHRGKATQKLVAYLQNLK",
    "response#3": "MSEQNTIVAKDIRGPEERTIAKGVFDSVAPKYDFLNSLLSLKIDSYWKKDAIKLTGLRPGDKVLDIAGGSGDLSRLFSKKVGKTGEVVLTDLNESMLRVGRDRLLDEGITVPAVQADAEKLPFPDDYFDCISVAFGLRNMTHKDKALAEMRRVLKRGARFLCLEFSKVWKPLEKAYDLYSFKVLPQLGELVAGDADSYRYLAESIRMHPGQEELKQMMEAAGLERVEYFNLAAGAVAIHRGFKY"
  },
  {
    "reference": "MQLKGRKIILGITGSIAAYKAAYLTRLLVREGAEVKVIITPLGKEFITPVTLATLSRNTVLCDFFNYDDGSWNSHVDLGMWADLFLVAPASANTMAKMASGICDNLLLTTYLSCRCPVMIAPAMDLDMFGHPATLHNMKVLLERGNIIIEPASGELASGLTGKGRMEEPENIINKLVSFFTAAGTGEGSPGEPGRLSSMNGRKVLVTAGPTHEPIDPVRFIGNHSSGKMGFAIAAELAGRGAEVVLVTGPVALELMHSRVKVVKVSSAAEMHNECTRWFPECDACIMAAAVADYTPLHTPETKIKRSGESYSIEMKPTVDILAELGRAKKKGQVLAGFALETDNEMENAKKKLHNKNLNFIVLNSLKEEGAGFHADTNKVTIINPDGSAVDYALKGKPEVAVDIVNHLSGLLK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that can modulate the overall flux of metabolites through the Cofactor biosynthesis, coenzyme A biosynthesis pathway, enhancing or suppressing its activity as needed.2. The protein design must be able to perform phosphopantothenate--cysteine ligase activity, FMN binding, metal ion binding in order to contribute to pantothenate catabolic process, coenzyme A biosynthetic process.3. The protein should be designed to have high stability and activity towards H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. The presence of Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.5. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A6. The presence of Mg(2+) is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "RVVLGVSGGIAAYKSCELLRRFTESGHDVTVVPTAAALEFVGAPTWAALSGKPVAADVWTRVEAVPHVHIGQHADLVVVAPATADIMAKAAHGLADDLLTNTLLTARCPVVFAPAMHTEMWEHPATQANVRTLRERGAVVIEPAEGRLTGKDTGKGRLPDPAEIFELALDALAFFAPKSLAGRHVLVSAGGTREYLDPVRFLGNRSSGLQGYALARAAAARGAEVTLVAANVSLPDPAGVKVVRVETTAQLRDAVLAGAPAADAIVMAAAPADFRPTDVSDAKIKKSEDGSAPAITLEQNPDILKEISQHRLREGQVIVGFAAETGDATGSVLELATAKLARKGCDLLVVNDVSGGAVFGSPDNEAVILAADGTEVSLPRGPKEALADAVWDAAAARLRLAGRHVLVSAGGTREYLDPVRFLGNRSSGLQGYALARAAAARGAEVTLVAANVSLPDPAGVKVVRVETTAQLRDAVLAGAPAADAIVMAAAPADFRPTDVSDAKIKKSEDGSAPAITLEQNPDILKEISQHRLREGQVIVGFAAETGDATGSVLELATAKLARKGCDLLVVNDVSGGAVFGSPDNEAVILSADGSEHLAGRHVLVSAGGTREYLDPVRFLGNRSSGLQGYALARAAAARGAEVTLVAANVSLPDPAGVKVVRVETTAQLRDAVLAGAPAADAIVMAAAPADFRPTDVSDAKIKKSEDGSAPAITLEQNPDILKEISQHRLREGQVIVGFAAETGDATGSVLELATAKLARKGCDLLVVNDVSGGAVFGSPDNEAVI",
    "response#2": "KKILLGVSGGIAAYKIPNLVRLLKKNGAEVQVLMTASAAEFASPLALATVSENKVLTEIFPDSAHEAKAEWTCHISLGEWADLFVIAPATANTIGKLASGLCDDMLSAAFLTLRPAKPRLIFPSMDGEMYFAKAVQRNLAQLKADGCEVIEPETGSLASGLTGMGRMPDPETIFDRIVNHLESTQDLKGKRIVITAGPTREALDPARYLSNRSSGKMGYALARTAARRGAEVTLVSGPVDIKAPSWVATIPVKTARQMHARVLELSEDADIIVKAAAVSDFKPKNYCRQKIKKNQGLTSIEVEENPDILSDLGESRRRGSVLVGFAAESRNHEAEGRRKLLQKKTDLIVVNDILSSKTGFDVDTNQVILLFDDQGEKELPIMSKREVAVRLLELIKDRLN",
    "response#3": "RVVLGVSGGIAAYKSCELLRRFTESGHDVTVVPTAAALEFVGAPTWAALSGKPVAADVWTRVEAVPHVHIGQHADLVVVAPATADIMAKAAHGLADDLLTNTLLTARCPVVFAPAMHTEMWEHPATQANVRTLRERGAVVIEPAEGRLTGKDTGKGRLPDPAEIFELALDTARLLLARPGGLPRDLAGERILVTAGPTREEIDPVRFISNHSSGKMGYAIARQARLRGAEVTLVTGPTAITPPLGVAVIRVESAEEMREAVLARLEETDIVIKAAAVADYRPRLRAQMKVKKTAAHLAIELEKNPDILAEIGTRKEGRVLVGFAAETEDLVANATLKLTLKNLDLVVANDVSQEGAGFHVDTNIAKVLWRDGEPAEVPLGSKDDLADTVWDLVAARFG"
  },
  {
    "reference": "KRKRMGHTVLNAESSRSHSVFNIRLVQAPTDSQGENVVQDRQNITVSQLSLVDLAGSERSSRTKNTGVRLREAGNINNSLMTLRTCLEYLRENQLSASNGLAPKKVPYRDSKITHMFKNYFDGEGQVSMIVCINPRIEDYDENMQVMKFAEMTQEVQIARATPMKQDLGLTPGRRKANKLFKIAVNNLNELGIPEAKDLEVDVGLVYSLGADFPAYEMDSPEAQIKIRELMHYLEQRIEKRKKLRANLDIKCDSFRQMLMNLDRDNLQLRTELASLKAVYKQERDRSAALEKKVRIHESSIDVLNNTLSKQERQIEELTFKLNEKENMLTQKEHEKEKQKKKFSSKLAVESDKNKREFELKLREQRAKLQERMRIKDEKLRLVSNILQSEELPSLPRSQSSENILNEKDRGAYTVRTEVSSVPATRTDIYATPRHGAAAANNRHRRSRSAGDKWLEHRAANPVPLGTIMQPYLKNRKSVTKLTDMKELTAHGTTKYCLVSQDADTDGDVETKLYKGNVIPTCGGGAQVVFNDVECLKQKSPVHSPTRK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Incorporate a signal peptide in the protein design.2. A protein that can perform ATP binding in male germline ring canal, midbody to support regulation of microtubule cytoskeleton organization, mitotic cytokinesis. The designed protein sequence is ",
    "response#1": "QGFCFNILCVGETGIGKSTLINTLFNTNFEELESSHFCPCVRLRAQTYELQESNVRLKLTIVNTVGFGDQINKEDSYQPIVDYIDDQFEAYLQEEVKIKRALFNYHDSRIHVCLYFIAPTGHSLRTLDLLTMKSLDNKVNIIPLIAKADTISKSELQKFKMKLMNELVINGVQIYQFPTDDDTTSKINGAMNGHLPFAVVGSMDEIKVGNKMVKGRQYPWGIVQVENENHCDFVKLREMLICTNMEDLREQTHMRHYELYRRCKLQEN",
    "response#2": "QGFCFNILCVGETGIGKSTLINTLFNTNFEELESSHFCPCVRLRAQTYELQESNVRLKLTIVNTVGFGDQINKEDSYQPIVDYIDDQFEAYLQEEVKIKRALFNYHDSRIHVCLYFIAPTGHSLRTLDLLTMKSLDNKVNIIPLIAKADTISKSELQKFKMKLMNELVINGVQIYQFPTDDDTTSKINGAMNGHLPFAVVGSMDEIKVGNKMVKGRQYPWGIVQVENENHCDFVKLREMLICTNMEDLREQTHMRHYELYRRCKLQET",
    "response#3": "QGFCFNILCVGETGIGKSTLINTLFNTNFEELESSHFCPCVRLRAQTYELQESNVRLKLTIVNTVGFGDQINKEDSYQPIVDYIDDQFEAYLQEEVKIKRALFNYHDSRIHVCLYFIAPTGHSLRTLDLLTMKSLDNKVNIIPLIAKADTISKSELQKFKMKLMNELVINGVQIYQFPTDDDTTSKINGAMNGHLPFAVVGSMDEIKVGNKMVKGRQYPWGIVQVENENHCDFVKLREMLICTNMEDLREQTHMRHYELYRRCKLQEA"
  },
  {
    "reference": "MGVLVKNLVEDLKLEVLNKGKDDIELNISDINRPGLQFSGFYNYFANERVQLIGKTEWSFLDVMSPELRKKRVAKFFQFETPCVIITRNLKPHKEVLENSRKYNRWLLNTSNISTRFTSKLMNYLDEKLAPETRLHGVLVDVYGIGILITGESGIGKSETALELIKRGHRLVADDAVDIKEIEGKLIGSSPYVTSGMLEVRGLGIIDVPSLYGLSSVLDIKTIGVIIHLEQWKKDQDYDRLGIDEINRDILNVPVRKITLPIRPGRNIAVIIEAAAANYRYNLNSNSSPVDTIGARMKEENLKNK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must have a Proton acceptor; for phosphorylation activity. Proton donor; for dephosphorylation activity that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "RIDFKEGKLSPEWIHLQNPEAKNYIFTKDGKLRLIATPVTLSDWKSPTFVALRQEHFDMEASAPVVLQKAGVNDEAGISVFMEFHSHYDLFVRQDKDRKRSVGLRYKLGEITHYAKEVSLPTDGEVELVVKSDINYYYFGYKVNGIYHDLGKMNTRYLSTETAGGFTGVVLGLYITSASKDSKAYADFEYFKYN",
    "response#2": "RIDFKEGKLSPEWIHLQNPEAKNYIFTKDGKLRLIATPVTLSDWKSPTFVALRQEHFDMEASAPVVLQKAGVNDEAGISVFMEFHSHYDLFVRQDKDRKRSVGLRYKLGEITHYAKEVSLPTDGEVELVVKSDINYYYFGYKVNGIYHDLGKMNTRYLSTETAGGFTGVVLGLYITSASKDSKAYADFEYFKYN",
    "response#3": "RIDFKEGKLSPEWIHLQNPEAKNYIFTKDGKLRLIATPVTLSDWKSPTFVALRQEHFDMEASAPVVLQKAGVNDEAGISVFMEFHSHYDLFVRQDKDRKRSVGLRYKLGEITHYAKEVSLPTDGEVELVVKSDINYYYFGYKVNGIYHDLGKMNTRYLSTETAGGFTGVVLGLYITSASKDSKAYADFEYFKYI"
  },
  {
    "reference": "MSMEPRKKRNSILKVRQAVETIEETVMNSGPSSTTTNRRVSFHNVKHVKQYDRDHGKILDATPVKEKITDTIGSDGILTPRGGNMDISESPACTSSFQVFGGGNLDKTMDMSLETTINENNETARLFETTRDPTLLYEKIVETTTKVTERIVSMPLDDTLAMFNTTNQEDKDMSVDRSVLFTIPKVPKHNATMNRTIPMDLDESKAAGGQCDETMNVFNFTNLEAAEMDTSKLDENNTMNAIRIPINSNVMPVDMDITEHHTLIEEKKNDTFGPSQLMDISAPQVQVNDTLAIFNSPRDICNKGLGVPQNLINIASNVVPVDMDITDQAVLNAEKKNDQFETSQLMDISIPKVLVNDTMAMFNSPKHVSKSSMDLEKTIEAADKSTKYPSIADEVEDLDMDMDITEQQPCEAGNQQNDGLQLQKEDLMDISVIRDSPAVNDTMAVFQSPARVKIGANNSIIDSQKSIVFGDEMSIDETQNDGTLTLPKSNVEVTTTNDVYTSLERQEENASENVSMINESSVHSEIDKKSFMLIEEERAFMHSSMIDVAQKLEDDGSSKTPVILASQSASLATKEPSALHNSSATLNNSMELDNNTLLKTMQITTCEDISMVHESIAVELNSNKEQEQFGDETLQKNDTSNTGANFTFQGHNETSQIMNNVDSEAVNTSKISTYSAFNLSINQSISKRRRSLLNSARESPRRVALENSIMSMNGQTMEALTEYRQNKTMQTSQDSMPSMSLNDSGRDILAMNTSVRSPHLNSSKTAAP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be designed to localize specifically to kinetochore in order to perform its role in protein localization to kinetochore more effectively. The designed protein sequence is ",
    "response#1": "LPNALVAPDEMEKNSNPLYSCQGEVLASRKDFRVNTCTPHPRGTFLLEPLGVSLMEALQPWNPKEPGRAEEC",
    "response#2": "LPNALVAPDEMEKNSNPLYSCQGEVLASRKDFRVNTCTPHPRGTFLLEPLGVSLMEALQPWNPKEPGRAEEC",
    "response#3": "KTSFFGKRASNGTARTSQYGAFGGTEKIKDPRPLHDKAFIQQCIRQLCEFLNENGYSQALTVKSLQGPSTKDFLKIFAFIYTFICPNYENPESKFEEEIPRIFKELGYPFALSKSSMYTVGAPHTWPQIVAALVWLIDCVKLK"
  },
  {
    "reference": "MGGKWSKRSIVGWSEVRERIRRARPAAERRIEPAAEGVGAASRDLDKYGALTSSNTAHNNADCAWLEAQEEEEEVGFPVRPQVPIRPMTFKAAFDLGFFLKEKGGLDGLIHSKKRQDILDSWVYHTQGFFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPREVEEANEGENNCLLHPMSQHGMDDADGEVLMWKFDSSLARRHMARELHPEFYKNC",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that contributes to suppression by virus of host autophagy, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I by functioning in host cell plasma membrane, host cell Golgi membrane, extracellular region, virion component, membrane with SH3 domain binding, GTP binding.2. Incorporating a Diacidic; necessary for CD4 internalization, Dileucine internalization motif; necessary for CD4 internalization, PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif is critical for the successful design of the protein.3. For general function, the protein need meet that Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPQVRERIRQTPAAATGVGAVSQDLDKHGAITSSNINHPSCVWLEAQEEEEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLDGLIYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGTRYPLTFGWCFKLVPVDPREVEEANEGENNCLLHPMSQHGMEDEDREVLMWKFDSQLARRHIARELHPEYYKDC",
    "response#2": "MGGKWSKRSVPGWTAVRERMRRTEPAAEGVGAASRDLEKYGALTSSNTPATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGTRFPLTFGWCYKLVPVDPAEVEEANEGENNSLLHPICQHGMEDEDREVLVWRFDSSLARRHIARENHPEFYKDC",
    "response#3": "MGGKWSKRSMPGWTRIRERMRRAKPDTEPAAEGVGAVSRDLEKHGAITSSNTPATNAACAWLEAQEEEEVGFPVRPQVPLRPMTYKGAFDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPEKVEEANEGENNCLLHPMSQHGMDDPEKEVLQWKFDSRLAFHHVAREKHPEFYKNC"
  },
  {
    "reference": "MGTGKKEKQRRIREGNTKDGNLRVKGENFYRDTKRVQFLNMYKGGRSVRNAKGDIIKAAPLQDTAAPTARVAPDRRWFGNTRVISQDSLSHFREALGENKRDSYQVLLRRNKLPMSLLNEKDSAESPTAKILETEPFEQTFGPKAQRKKPRIAASSLEELISSTSTDNKTFEEKQELDSTLGLMGKQEEEDGWTQAAKEAIFHKGQSKRIWNELYKVIDSSDVVIHVLDARDPLGTRCKSVTDYMTNETPHKHLIYVLNKCDLVPTWVAAAWVKHLSKERPTLAFHASITNSFGKGSLIQLLRQFSQLHKDRHQISVGFIGYPNTGKSSIINTLRKKKVCQVAPIPGETKVWQYITLMKRIFLIDCPGIVPPSSKDSEEDILFRGVVRVEHVSHPEQYIPGILKRCKRQHLERTYEISGWKDSVDFIEMIARKQGRLLKGGEPDESGVSKQILNDFNRGKIPWFVPPPEKDKIEEKEPGDKKRPAEENQEDQEEEEKEQEEQEEPVSKKAKKE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein localizes to the nucleolus. The designed protein sequence is ",
    "response#1": "FGYITPVPLNMELIDISLSKTQKRTPTVIHPQYNIVKIRMFYMRKVKHAGNEFASRLGTILTDFPRIEDIHPFYGDLINVLYDRDHYKLALGHVNAAKNGIEKVSKEFVKLLKFADSLYRCKQLKRAALGRMASAAKKLGKTLEYLEEVRMHMSRLPSIV",
    "response#2": "MCEDVRSCVQCQAWGTGEKKGQKCKDCPYKIQMVDELQKAKEVSEHCSFQDEEDDCTYHYTLEGEPSTMRNNTILVQKKKECPPA",
    "response#3": "FGYITPVPLNMELIDISLSKTQKRTPTVIHPQYNIVKIRMFYMRKVKHAGNEFASRLGTILTDFPRIEDIHPFYGDLINVLYDRDHYKLALGHVNAAKNGIEKVSKEFVKLLKFADSLYRCKQLKRAALGRMASAAKKLGKTLEYLEEVRMHMSRLPSII"
  },
  {
    "reference": "MGACCSCLGNRGGDGSHTQLLLAENEREAISALLQYLENRSDVDFFSNGPLRALSTLVYSENIDLQRSAALAFAEITEKDVREVNRDVLEPILILLQSADSEVQRAACGALGNLAVNTENKILIVEMGGLEPLIRQMMSTNIEVQCNAVGCITNLATQDDNKSKIAKSGALIPLTKLAKSKDIRVQRNATGALLNMTHSGENRQELVNAGAVPVLVSLLSNEDADVQYYCTTALSNIAVDEVNRKKLASTEPKLVGQLVHLMDSPSPRVQCQATLALRNLASDSGYQVEIVRAGGLPHLVQLLTCNHQPLVLAAVACIRNISIHPLNEALIIEAGFLKPLVGLLDYTDSEEIQCHAVSTLRNLAASSEKNRTALLAAGAVDKCKELVLKVPLTVQSEISACFAILALADDLKPKLYESHIIDVLIPLTFSENGEVCGNSAAALANLCSRVSNEHKQYILNNWAQPNEGIYGFLIRFLESGSPTFEHIALWTILQLLESNNTEINALIKENETILAGIKNLSASQQQIQQSQIGQTTTTTTTNITNNNTNTNTNTNTTTSTSNEDQFEDPKVELFNLTQQILQILG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Vacuole inheritance has a role in the regulation of hyphal cell division..2. The ideal protein would be one that plays a critical role in nucleus-vacuole junction assembly, hyphal growth through protein-membrane adaptor activity, identical protein binding within fungal-type vacuole membrane, Myo2p-Vac17p-Vac8p transport complex, extracellular vesicle. The designed protein sequence is ",
    "response#1": "ISYEKIARTAYQEGRLILSTKLLDFEPLAKHQVMLLLNMEAYEQALKKVIETMDNNLIIYVVLQIKQQMAIASFFQILNEYPDAVKVYVEFAKKNDRKTLHDFFYQDDNKQGIAVLAVEDTLKTATVNQRITSLKSAAKVCSESKELSLEEKCLGDEIKLLQLQQTYEDQFTGNFVGLTVNEVVVKLIQINQTQRANKLRSDFQIPEKRFAWLKLRALVAIRDWVQIEQWAGSMRRSPIGFEPFVTEILSAGNKKEAAKYIPRCTQLTTQEKVDMWVQVGDVKSASEEAAKSKSGSVLGDLLERVQTMPESRFVQNALK",
    "response#2": "WGLARISHKSVKYDDIGKYVYDSSAGDNITAYVVDTGVSIHHVEFEGRASWGATIPSGDVDEDNNGHGTHVAGTIASRAYGVAKKAEIVAVKVLRSSGSGTMADVIAGVEWTVRHHKSSGKKTSVGNMSLGGGNSFVLDMAVDSAVTNGVIYAVAAGNEYDDACYSSPAASKKAITVGASTINDQMAYFSNYGSCVDIFAPGLNILSTWIGSNTSTNTISGTSMATPHVAGLSAYYLGLHPAASASEVKDAIIKMGR",
    "response#3": "NITAYVVDTGVSIHHVEFEGRASWGATIPSGDVDEDNNGHGTHVAGTIASRAYGVAKKAEIVAVKVLRSSGSGTMADVIAGVEWTVRHHKSSGKKTSVGNMSLGGGNSFVLDMAVDSAVTNGVIYAVAAGNEYDDACYSSPAASKKAITVGASTINDQMAYFSNYGSCVDIFAPGLNILSTWIGSNTSTNTISGTSMATPHVAGLSAYYLGLHPAASASEVKDAII"
  },
  {
    "reference": "MTSTLDVSKVLGLLDQVLAERKASGDPEASYVAQLHHKGLNKILEKVGEEATETILAAKDAERSGNTDLLVCETADLLFHAMVMLSHLGSNTDAVMAELARRFDLSGLEEKASRK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. We need a protein that can perform 1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-   ribosyl)-5'-AMP + diphosphate + H(+) with high efficiency.2. The protein must exhibit phosphoribosyl-ATP diphosphatase activity.3. The protein should be optimized for activity in L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9, L-histidine biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MMAWMNKESLEKTLITGEVHYWSRSREKLWHKGETSGHFQILKGIRFDCDSDVMLLSIEQVGSIACHTGARSCFA",
    "response#2": "RTHDVPVYVEYGQAQLGIVGYDVLRERYAGTEARVAHLLDLQFGHCRMSVALPQDSPYHSAAQLPAHARVASKFVGCAREYFDRLDLPVELISLYGSVELAPLTGMADAIVDLVATGRTLRENGLIERDCLFESTARLIAHPISYRVNQQPIRELITQIS",
    "response#3": "RTHDVPVYVEYGQAQLGIVGYDVLRERYAGTEARVAHLLDLQFGHCRMSVALPQDSPYHSAAQLPAHARVASKFVGCAREYFDRLDLPVELISLYGSVELAPLTGMADAIVDLVATGRTLRENGLIERDCLFESTARLIAHPISYRVNQQPIRELITQIL"
  },
  {
    "reference": "MKLSEVLKKVKPLEIVNGADVEITGVCTDSRKVKPGNLFVAVRGTQVDGHDFIAAAVSLGAAAVLMDAVIEKYVTDGVVFVRVANTEDAVGPVATTFFGDPSRHLKLVGVTGTNGKTTIATLLYTLFRKLGYKCGLCSTVCNYIDGEAVPADHTTPDPITLNELLARMVATGCDYAFMECSSHAIHQKRIGGLEFAGGIFTNLTRDHLDYHKTFENYRDAKKAFFDSLGKGAFAITNADDKNGMVMVQNTQAAVRTYSTRTAADFKGRILEESIEGMLISIDGREVSVHFVGRFNVSNLLAVYGAAVMLGESADEVLRVLSDLRPVNGRFEAMRAPAGYSAIVDYAHTPDALVNVLDAIREIVHGRGRVITVCGAGGNRDKGKRPLMAREASTRSDRLILTSDNPRFEEPQAILDDMLAGLDEDLRRKTLVIVDRREAIRTACMMAQPGDVILVAGKGHEPYQEVKGVKHHFDDHEELREAFGLSSKA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein contains novel Mur ligase N-terminal catalytic domains that confer a unique function or activity.2. The protein should be optimized for stability and expression in cytoplasm where it will participate in cell wall organization, cell division.3. The protein should have a low Km value for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "MRLDSRSVLLDDLFVAVHGHSTNGHHFISQAINQGAAAVIAETTEKRNHGKINHIQGIPVIYIMNLSHCLSAIAGRFYGNPSQKLKLVGVTGTNGKTSCTYWLAELQNILGKKTGLIGTLGYGYKKLKAHAYTTPDAISNHRIFKELKLNHVSSVVMEVSSHALAQGRVNSIHFDVAVFTNLSRDHLDYHLSFKNYFNEKKKLFHFPSLKVAVINIDDAYGRKLKTSLAKKKIKVITYGIKNGNMRATHINYSASSTTFKLHYKKEIHEVNIPLVGEFNVYNILAVISTLLSLGYDFDEIVSLMPQLRGASGRFEQVISDNRIVGIVDYAHTPDALQNVLETITNLRQDNEQGVLPRIITVVGCGGNRDAAKRPIMAQIAGKFSDRVILTSDNPRFEDPMAILEQMQAGLSPLDYKKAQTIEDRRKAIQTAVSLARPNDIILVAGKGHEDYQILKDRTIHYDDREVARDILQERLLG",
    "response#2": "VTGTQGKTTTTRLAELALESAGVPAAVIGTVGTRINGVDIKTALTTPEAPDLHGLFAVMVEKQVDACAMEVSSHALVLGRVDGVTFDVAVFLNLGRDHLDFHADLDEYFAAKRKLFARDRARLALVNIDDEHGRLLVHQLASDPRGIPVRTFSITGARADWQASAIEAGADGSRFTVTGPGNVRVVTSVPIAGAFNVSNALAAIAACRELGRDFADVMIALPHLVGVPGRLEQVETGRDFHVVVDYAHKPDAVEAALSTLRPLTAQAGGRLICVIGAGGDRDTGKRPVMGEIAARLADLVIVTDDNPRSEDPAVIRAAVLEGTRDGAGEVREVGDRREAIRVALSSAGPGDIVLVAGKGHETGQIVGDVVLPFDDADVAREALG",
    "response#3": "MRLDSRSVLLDDLFVAVHGHSTNGHHFISQAINQGAAAVIAETTEKRNHGKINHIQGIPVIYIMNLSHCLSAIAGRFYGSPSRSLKLTGVTGTNGKTSVATLLFRLFTSLGYRCGLLSTVQNQIGDTVIPATHTTPDSITTNELLTQMLAQGCTHVFMEVSSHAVVQERIAGLHFVGGIFTNITHDHLDFHGTFDKYIKAKKGFFDQLPRSAFALTNADDKRGAVMLQNTLARKESYSLQTLGTFKGKVLSDGLFGLEMLVDERQVWFKLIGRFNAYNLLAVYGAFGILLDLGEDVLVTGTNGKTTIATLLYQLFKKAGFKVGLLSTVNIMVDATVYKATHTTPDSVTLNKYLKLMNEEGVEFCFMEVSSHGIHQKRIEGLTFAGGVFTNLSHDHLDYHNTFKEYRDVKKEFFDALPSSAFALVNKDDKNGMFMLQNSKAKAYSYALKSYADYRGQILENSLGGLLLKLNDHEVWSKLIGSFNAYNVLAIYATAIALEEDPLQVFPRLAAVPGRVQTFNSPKGYSVIVDYAHTPDGLENVLQAINQFAKSRIITVFGCGGDRDNKKRPIMGEIAAKYSTDVIITSDNPRTEEPNSIIEQIEVGTKKVTDEYIKIVDRKKAIYAALSRATSGDVVLIAGKGHETYQEVQGVKHPFSDREWARLSLQE"
  },
  {
    "reference": "MRTHGLRLVLLALLPTMALGLLEGLYCGKENCYDVLGVTRESSKSEIGKAYRQLARRYHPDLHRGAEAKAAAETQFKLVATAYEILRDEESRTDYDYMLDNPDAYYAHYYRYYRRRVAPKVDVRVVIVVVLTIVSVIQYYSGWQRYDSAIKYFATVPKYRNQALEIARDEIQEKIQKKGKNRMSKNDQRDELERIIRRVIEEKMDVKGGYAKPTLWDVLWVQLIICPYTILSFIVWHAQWFWRYTVMKQPYGREQKLYLIRRHLGMGQHQFEAQEDKLIEEYLHLKLWKRENFVAWKAEQEEEMKKKLAENPRYKAYRRYMKNHGPGRITFED",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Design the protein with a signal peptide incorporated.2. The designed protein have J domains with specific sequence motifs that are crucial for its activity.3. The protein should be engineered to have a higher affinity for a specific molecule or receptor in endomembrane system, endoplasmic reticulum membrane that is critical for protein folding. The designed protein sequence is ",
    "response#1": "AVIYLSRSSKYTGNYVMHQTLSAYYYLMKPSLAPSKVMEVFTKAAEYMEIPVRRTDDEPLQKLFMSVRSELNLDLKNMKQEQAKFWKQHPAIVKTELLIQAQLTRESGVLSPALQGDFRRVLELAPRLLEELLKMAVIPRTAQGHGWLRPAVGVVELSQCIVQAVPLSARKSSGVSSEGISPFLQLPHFSDAVVKKIARKKVKSFQDLQEMRLEDRSELLTQVAGLSATDVEDIEKVLEMMPSITVDITCETEGEEGIQEGDIVTLQAWVTLKRPNGLVGALPHAPYFPFHKEENYWVLLADSVSNNVWFSQKVSFLDEGGAITAASKAISESMEGSGAGVKETNDAVREAIEKVKGGSRLVMGKLQAPAEGTYNLTCFCLCDTWIGCDKKQALKC",
    "response#2": "NYVMHQTLSAYYYLMKPSLAPSKVMEVFTKAAEYMEIPVRRTDDEPLQKLFMSVRSELNLDLKNMKQEQAKFWKQHPAIVKTELLIQAQLTRESGVLSPALQGDFRRVLELAPRLLEELLKMAVIPRTAQGHGWLRPAVGVVELSQCIVQAVPLSARKSSGVSSEGISPFLQLPHFSDAVVKKIARKKVKSFQDLQEMRLEDRSELLTQVAGLSATDVEDIEKVLEMMPSITVDITCETEGEEGIQEGDIVTLQAWVTLKRPNGLVGALPHAPYFPFHKEENYWVLLADSVSNNVWFSQKVSFLDEGGAITAASKAISESMEGSGAGVKETNDAVREAIEKVKGGSRLVMGKLQAPAEGTYNLTCFCLCDTWIGCDKKQAC",
    "response#3": "AVIYLSRSSKYTGNYVMHQTLSAYYYLMKPSLAPSKVMEVFTKAAEYMEIPVRRTDDEPLQKLFMSVRSELNLDLKNMKQEQAKFWKQHPAIVKTELLIQAQLTRESGVLSPALQGDFRRVLELAPRLLEELLKMAVIPRTAQGHGWLRPAVGVVELSQCIVQAVPLSARKSSGVSSEGISPFLQLPHFSDAVVKKIARKKVKSFQDLQEMRLEDRSELLTQVAGLSATDVEDIEKVLEMMPSITVDITCETEGEEGIQEGDIVTLQAWVTLKRPNGLVGALPHAPYFPFHKEENYWVLLADSVSNNVWFSQKVSFLDEGGAITAASKAISESMEGSGAGVKETNDAVREAIEKVKGGSRLVMGKLQAPAEGTYNLTCFCLCDTWIGCDKKQALKC"
  },
  {
    "reference": "MSKKSKAPLSLSDCLCQICMEIFVEPVTLPCSHTLCNYCFQMTVEKASLCCPFCRRRVSSWARYNTRRNTLVNWELWEKIQKNYPEECERRINGQDLDEEICSPKPRHQLSKPGELRQEYEAEISKVEAERRAHEQEENKASEEYIQRLLAEEEEEHRLAEERRKEMEKQLKQDEELAWQLSNSLNEDPEEHVPGSPSPAHSLSLQTSPLNLSKAKKKPSNSGDIQKYLSPKNYGMLGSASFSSTTGRGRNNSISVETNSNEGSSSALQDEQEEMPTLSPQLTNVNKESAAKDSFLESCMNYFSASASGETTTVKQEKTPGPNGLEDGIPGVLHGSTHGEGSRTVVLTSKGEDGGIETDNGNLTHIQRANLENSDDTCTSGQLGVGCGMSDSQTALCDPGKEPGHAGGETPQRLQNSKETPKRKLLEAPADAMIDLGMFDKRRRTSSESVEDQEEQMSNFNLQTQRAFEQEFYERRRQEEQDRLLALQLQKELDKEERTLNRQKGSPDEYLLRTKPPRSLKDSSARKATSRVAKDSKGSKTQAETNHHKARKGSCNENWQSPAKVRVKSPSIKEGKVLNCVVNTSDTNDICSLPKNKQKTILQMFKSPVAE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should take into account the physiological conditions of the protein ubiquitination pathway.2. The protein have enhanced S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +   [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-   cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine..3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.4. The designed protein have RING-type domains with specific sequence motifs that are crucial for its activity.5. The designed protein should contain at least one LR motif, MIU motif motif to achieve its intended function.6. The designed protein should be optimized for ubiquitin-protein transferase activity, chromatin binding activity in order to improve response to ionizing radiation, chromatin organization, double-strand break repair.7. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "MWIQVRTMDGKVAHSVGSLSRLTKVEELREKIQELFHVEPGRQRLFYRGKQMEDGHTLFDYDVRLNDTIQLLVEFSDGVTCIDFVLAWEENVTQAKLDDPEDKSSNTTGDVHKQWRTEFLSRLQNAGLHQEIKEVVQGKTKTIYVLLSAPWNVLCYYAEEISLRVPLQIETSRDSNWSETVLRKLHIPNILAQDVPNPPPDYYTCQFRSNKLERFLGHENKDTFFKMTQRHQIGVTKTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQINNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNSLMWIKENDPRILELTFCLDEDVFGQKSQHELKTGGANIEVTNENKDKTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQINNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNSLMWIKENDPRILELTFCLDEDVFGQKSQHELKTGGANIEVTNENKDGLYKANEYVDARDTDVGSWFEAKVVRVTKKPPSQDAPCSSTSTLEEDDDVIYHVTYDDYPEYGVVQMSSQNVRARAREIIRWQELKEGQEVMLNYNPDSPKERGFWYDAVITRKNQTRTSRELYANVRLGSSSLSNCRIMFVDEVFKIERPREHLRIVVLSGQSLAKKDIFGASDPYVRIDLNTINGDINIDSVLTKTKKKTLNPTWTEEFIFRVKPSEHKLVFQVFDENRLTRDDFLGMVELTLVNLPTEQEGRT",
    "response#2": "MWIQVRTMDGKVAHSVGSLSRLTKVEELREKIQELFHVEPGRQRLFYRGKQMEDGHTLFDYDVRLNDTIQLLVLAWLVTFLEYWKRSCSVLAHKWDCSEFEETEERPRPEFTAMAPMTVRNPVTGVEEPHFPEISRFRRMVTGNMVIILMVAVVLMFLMGIILYRTILSIIIYNSKNDFFIFSAGRIASLTGSVLNLVVIMLLSKVYTRLAQVLTSWVNTRGLYKANEYVDARDTDVGSWFEAKVVRVTKKPPSQDAPCSSTSTLEEDDDVIYHVTYDDYPEYGVVQMSSQNVRARAREIIRWQELKEGQEVMLNYNPDSPKERGFWYDAVITRKNQTRTSRELYANVRLGSSSLSNCRIMFVDEVFKIERPL",
    "response#3": "MWIQVRTMDGKVAHSVGSLSRLTKVEELREKIQELFHVEPGRQRLFYRGKQMEDGHTLFDYDVRLNDTIQLLVSEESHLCGVSGPVGNQPEGTMSDTTIHASLPGYPNCGTIVIDYNMKGGIQTNSHPNPGQRYGPAQRTAYLPDNEEGREILQLLKRAFNQKLIFTVGQSHTTGAQNVITWNDIHHKTSMMGGPTQFGYPDYSYLQRVRSELKAKCSLCGSKQDPDKQLMCDDCDHAFHLYCLNPPLSSVPTETEWFCPKCIPVVTTLYEYFSHTRKEEIKELHKRFGARIRTKGHEEDNTLIHISSDRSPALLQTACDSFIRTFQKATENLTEEIVPITNSYTLEETIMKLNGKFRNLLAKKEGNQLLLRGPGSEILAAQKGLYKANEYVDARDTDVGSWFEAKVVRVTKKPPSQDAPCSSTSTLEEDDDVIYHVTYDDYPEYGVVQMSSQNVRARAREIIRWQELKEGQEVMLNYNPDSPKERGFWYDAVITRKNQTRTSRELYANVRLGSSSLSNCRIMFVDEVFKIERPGEY"
  },
  {
    "reference": "MTVERQLHKFGGSSLADAECYQRVVNILKEYSQPNDLVVVSAAGSTTNQLIAWLGALEKDGRVAHEMLQELRQYQTSLIESLLPEERAAGLLDALNTEFSGLGELSFPLTSAQKSSAMGHGELWSARLLAELLNHNNLEAVSQDSRDFLRAEHGAQPEVDRARSYPLIKESLAQHRQCRVVITGFMAKNDDGETVLLGRNGSDYSATVIGALAEATRVTIWSDVAGVYSSDPRKVSDACLLPLLRLDEASELARLAAPVLHSRTLQPVAQSAMELHLRCSYQPESGSTRIERVLASGRGAKIITSLDEVLLVKVDFGHGHDFDHHSKEVLTTLTRAQLSPLAFEAQEDQGCLLLAYTSEVAGDALSHLQDVSIGAEIKLKEGYSLVAAVGAGVTNNPNHCYGFYQQLKNGPVEFISEAESGLSLVAILRKTDTSRLITGIHSQLFQAQKRVAIALCGKGNIGSSWLELFSEQKDELEKRYGKTFDLVAVVDSQTYWLDFSGIDANEVFSRFDDEAICNDGTEWLDKLAHQQKYDEVIVLDVTASEALSDQYVSIAEHGFHLISANKVAGSAPGEVYHQVQDAFSKTGRHWFYNATVGAGLPVNHTVRDLRESGDDILALSGIFSGTLSWLFQQFDGQVPFSDLVDLAWQQGLTEPDPRCDLDGSDVMRKLVILARESGLELEPENVKVESLVPESLRDLPLDDFLDNGQILSELLAERLEKAQREDKVLRYVARLEKDGKASVGVEALPREHALANLLPCDNIFAIES",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Ideal protein should have a binding affinity for key enzymes in the Amino-acid biosynthesis, L-threonine biosynthesis, L-threonine from L-aspartate: step 3/5 pathway to enhance the overall efficiency of the pathway.2. We are looking for a protein with broad substrate specificity for L-homoserine + NADP(+) = H(+) + L-aspartate 4-semialdehyde +   NADPH.3. The designed protein should contain one or more Homoserine dehydrogenase catalytic, Aspartate/glutamate/uridylate kinase, Aspartate/homoserine dehydrogenase NAD-binding domains that are essential for its function.4. The protein design must be able to perform homoserine dehydrogenase activity, NADP binding, ATP binding, aspartate kinase activity in order to contribute to phosphorylation, lysine biosynthetic process via diaminopimelate, threonine biosynthetic process. The designed protein sequence is ",
    "response#1": "MKILKFGGTSLANATKFLTVANIIEKNFKKEQVAVVLSAPENVTNSMIKIIEKIIEERDVSQNIHSVKSIFLDLIFDIYKATKNFSYKTIKKIIDEKFENLTSIFNGIRFLNQCPDNVRAKIVCCGELLSITIMDGILKSMSYNVTIIDPIENLVAQGDYLDSTIDIQTSKKKIRSINIPKNHIILMAGFIAGNKKKKLVVLGRNGSDYSAAILSVCLKGNVCEIWTDVDGIYTCDPKKVKNAKLLKSLSYKEAMEFSYFGAQVLHPKTIFPIKKFKIPCLIKNTKNPNAVGTMIGTGGIGNTLLKQIYQKKNYFKKKNIILKICGIFNTKYSLININGLNIKNWKQNIEYNPNINIIKILLNNFKKYKFINPVIIDCTSSQEIANQYVNFFNCGLNIVAANKKANSSKMDYYNQIHFLANQLNLKFFYGVGGVGKTLLKQLNSQQYWLKTKNIDLKVCGIANSKKIFQDMNGIDLKNWKYNVLKSNELFNIKNLTCLPKKNALINPIIVDCTSDQEIANQYPKLLDHGYNIVTSNKKANTSSLKYYQEIRHSASRANKKFLYAGKGNIGSSWVELFSKQKAELERRRGMSFDLIGMLDQQRYWLDFQGLDPETALSCFNDEAVANDGKEWLEKLSHQTIFDEVIVLDVTASEVLARQYPDIAERGLHLISANKVAGSASGHFYHKVQDAFAKTGRYWLYGTGGIGNTLLKQIYQKKNYFKKKNIILKICGIFNTKYSLININGLNIKNWKQNIEYNPNINIIKILLNNFKKYKFINPVIIDCTSSQEIANQYVNFFNCGLNIVAANKKANSSKMDYYNQIHFLANQLNLKFFY",
    "response#2": "GKGNIGASWLRLFYQEKAELEKRRGMNFELVAVVDSQRYVFNEAGLNEQDVFEHYQDSAINHDGQNWLASVDNLHRYDDTIVLDVTASPEVATQYLAFAQHGMHLISANKVAGSAPSDYYNQVKDAFAKIGRHWFYEGYAPVIENLKNLLYSGDKIIHFRGILSGSLSFIFGKLEENVLLSEATKQAQKLGLTEPNPKDDLSGVDVARKLLILAREIGLKLELKDIEIKPILPENFNHITHAEDFMIKLRELDEIFDDKIKKAKKIGKTLRLVGIIKKEGQCQVKIDEIDEQDPFQ",
    "response#3": "MKILKFGGTSLANATKFLTVANIIEKNFKKEQVAVVLSAPENVTNSMIKIIEKIIEERDVSQNIHSVKSIFLDLIFDIYKATKNFSYKTIKKIIDEKFENLTSIFNGIRFLNQCPDNVRAKIVCCGELLSITIMDGILKSMSYNVTIIDPIENLVAQGDYLDSTIDIQTSKKKIRSINIPKNHIILMAGFIAGNKKKKLVVLGRNGSDYSAAILSVCLKGNVCEIWTDVDGIYTCDPKKVKNAKLLKSLSYKEAMEFSYFGAQVLHPKTIFPIKKFKIPCLIKNTKNPNAVGTMIGVSGPGMNSMVAVASRVLSSMSLKKIRITLITQSSSEFNISFCTSQEYVKKIKNTLENEFQLHKFGGSSLANPECYKRVVTILSKYSEDNDLVVVSAAGDTTNRLIEWTHALYKDGRLAHELLMGIRDFQSALIEALLANPEPYLQTLHQEISALGELAAPLTEAESAAALAHGELWSSRLLAQLLVDSQLPAVALDARAFLRAERATQPEVDRARSWPLLREALAQHSGHRAIITGFMARNDQGETVLLGRNGSDYSATVIGALAEAHKVTIWSDVAGVYSADPRKVSDACLLPLLRLDEASELARLAAPVLHSRTLQPVSQSAVNLELRCSYQPETGSTRIERVLASGRGAKIITSLDDVMLIDINFGHQHDFERAAEEALQALIRAQLQPLAWDSQPDQTCIRLAYTQEVASGALRYLQELALAAEIRLKEGYVMVAVVGAGVANNPIHSHGFYQQLKNAPVEFIRESESQLSMVAVLRQTDTALLVQNIHTHLFQAQKRVALVLCGKGNIGSSWVELFSKQKAELERRRGMSFDLIGMLDQQRYWLDFQGLDPETALSCFNDEAVANDGKEWLEKLSHQTIFDEVIVLDVTASEVLARQYPDIAERGLHLISANKVAGSASGHFYHKVQDAFAKTGRYWLYNATVGAGLPVNHTVQDLKQSGDDIHGLSGIFSGTLSWLFQQFDGSIPFIDLVDLAWQQGLTEPDPRSDLDGSDVMRKLVILARESGLALEPEQVKVESLVPEALRALPLDDFFEQGHILSDALAERLNKAQREDKVLRYVARLEKNGQATVGIEALPKEHALANLLPCDNIFAIE"
  },
  {
    "reference": "MKKNTPLLLLSTIASFNVFGAQEIATLAGGCFWCTESDMEKLNSVGDVISGYSGGNVPNPTYEQVSSGTTGHIESIQFNFDNSVHSYTDILDYFIRHIDPTDPNGSFVDRGKHYRPAIFYHNQTQKEQAELFLKEIDDARIYPKPIAIELIPYKKFYPAEEYHQDYYKKSKIKYSYYRYRSGRDQYIEEVLGDNLLTLKDLIKSKNVKSYARPSDDEIKNKLSKVQYYVTQKDGTEKPFDNKYWDNKKEGIYVDIVSGEPLFSSTDKYKSGTGWPSFTKPINKGYITLHEDNTLFHTRTEVRSKFADSHLGHVFKDGPAPTGLRYCMNSASMRFIPKENLEKEGYSEYTYLFN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Design the protein with a signal peptide incorporated.2. The designed protein should have peptide-methionine (R)-S-oxide reductase activity to facilitate protein repair.3. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "MEEYFSRVPGVLNTISGYANGNTKDPTYEDVCSGRTGFAETVHVVYDPAVVTLRILTQLFFKIIDPVSVDRQGNDRGTQYRTGIYYTDEEDMAEILTVIAAVQKDYEKPLAVEFETLKSFYPAEDDHQDYLKAHPNGYCG",
    "response#2": "MEEYFSRVPGVLNTISGYANGNTKDPTYEDVCSGRTGFAETVHVVYDPAVVTLRILTQLFFKIIDPVSVDRQGNDRGTQYRTGIYYTDEEDMAEILTVIAAVQKDYEKPLAVEFETLKSFYPAEDDHQDYLKAHPNGYCG",
    "response#3": "MEEYFSRVPGVLNTISGYANGNTKDPTYEDVCSGRTGFAETVHVVYDPAVVTLRILTQLFFKIIDPVSVDRQGNDRGTQYRTGIYYTDEEDMAEILTVIAAVQKDYEKPLAVEFETLKSFYPAEDDHQDYLKAHPNGYCG"
  },
  {
    "reference": "MGGKWSKRSKVEWPAVRERMRQAPAAEEVGAASRDLAKHGALTTSNTAHNNADCAWLQAQEEEEEVGFPVRPQVPLRPMTYKAAFDLSFFLKEKGGLDGLIHSKHRQDILDLWVYNTQGFFPDWQSYTPGPGIRYPLTFGWCFKLVPVDPKEVEEANEGEDNCLLHPMHLHGIEDPDKEVLKWVFDSSLARKHTAREKHPEYYKDC",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be involved in suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I by localizing to host cell Golgi membrane and exhibiting GTP binding, SH3 domain binding.2. For general function, the protein need meet that Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.3. Incorporating a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif is critical for the successful design of the protein. The designed protein sequence is ",
    "response#1": "MGGKWSKRCMAGWSAVRERMRRAEPAADGVGAASRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPEKVEEATEGENNSLLHPMSQHGMDDPERETLVWKFDSRLAFHHVARELHPEYYKN",
    "response#2": "MGGKWSKNSMVGWPAVRDRIRRTDPAAEGVGAASRDLEKHGALTSSNTAANNPDCAWLEAQE",
    "response#3": "MGGKWSKRSAPGWATIREKMRRTEPAAEGVGAASRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPEQVEKANEGENNSLLHPMSLHGMDDPEKEVLEWRFDSRLAFHHIAREMHPEYYKNC"
  },
  {
    "reference": "MSEAITGAERYKSGVIPYKKMGYWEPDYVPKDTDIIAMFRITPQAGVEPEEAAAAVAGESSTATWTVVWTDRLTACELYRAKAFRTDPVPNTGEGTKTEQQYFAYIAYDLDLFEPGSIANLTASIIGNVFGFKAVKALRLEDMRIPVAYLKTFQGPATGIIVERERLDKFGRPLLGATTKPKLGLSGRNYGRVVYEGLKGGLDFMKDDENINSQPFMHWRDRFLYCMEAVNKASAATGEVKGHYLNVTAGTMEEMYERAEFAKSLGSVIIMIDLVIGYTAIQSMAKWARKNDMILHLHRAGNSTYSRQKNHGMNFRVICKWMRMAGVDHIHAGTVVGKLEGDPLMIKGFYDTLRDRHTPVSLEHGLFFEQDWASLNKVMPVASGGIHAGQMHQLLDYLGEDVILQFGGGTIGHPQGIQAGAVANRVALEAMIMARNEGRDYVKEGPQILEEAAKWCTPLKLALDTWKDITFNYESTDTADFVPSETASV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit monooxygenase activity, magnesium ion binding, ribulose-bisphosphate carboxylase activity.2. For general function, the protein need meet that Both reactions occur simultaneously and in competition at the same active site.3. The desired protein should be able to perform D-ribulose 1,5-bisphosphate + O2 = (2R)-3-phosphoglycerate +   2-phosphoglycolate + 2 H(+)4. The designed protein should have a high specificity for substrate, Mg(2+) binding, with minimal off-target effects.5. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "YWTPDYTPLDTDLLACFKVVPQEGVPREEAAAAVAAESSTGTWTTVWTDLLTDMEFFKGRAYRIEDVPGDKNAFYAFIAYPLDLFEEGSVVNVLTSLVGNVFGFKAVRSLRLEDLRFPIG",
    "response#2": "ISDMWRILGRPIENGGYIAGTIIKPKLGLRPEPFAAAAYQFWLGGDFIKNDEPQGNQVFCPLKKVLPLVYDSMKRAQDETGQAKLFSMNITADDHYEMMARADFGLETFGPDADKLAFLVDGFVGGPGMITTARRQYPNQYLHYHRAGHGMITSPSAKRGYTAFVLAKISRLQGASGIHVGTMGYGKMEGEGDDRNIAYMIERDEAQGPVYFQKWYGMKPTTPIISGGMNALRLPGFFENLGHGNVINTAGGGSYGHIDSPAAGAISLKQAYECWKAGADPIEFAKEHKEFARAFESGRDH",
    "response#3": "GIQLERDRLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENINSQPFMRWQHRFEFVMEAVHKATSETGERKGHYLNVTAPTPEEMYKRAEFAKSLGAPIIMHDFLTAGFTANTGLANWCRENGMLLHIHRAMHAVLDRNPMHGIHFRVLTKCLRLSGGDHLHSGTVVGKLEGDREATIGWVDLMREPFVPENRARGIFFDQDWGAMPGVMPVASGGIHVWHMPALTAIFGDDACFQFGGGTLGHPWGNAAGAHANRVALEACVEARNQGRPVEREGREILTEAAQHSPELKIAMETW"
  },
  {
    "reference": "MSVNLLGLDAQALQAFVVEIGEKPFRARQLMRWVHGTGESSFADMTDLAKSLRAALQHRAVVVAPRVISDRVSEDGTRKWLLDVGNGNAVETVFIPEPSRGTLCVSTQAGCTLACEFCSTGKQGFNRNLTTAEIVGQLWLANRTLRVAPPGDPETRGDVDAGPRHLERNEAPKERGGDVRVACGDRAVSNVVLMGMGEPLLNFDNVVPALRLMLDDNAYGLSRRRVTVSTSGVVPLIDRLRDECPVALAVSLHAPNDPLRDQLMPINRKYPLRELLDACIRYLGRSPRDFITFEYVMLAGVNDGRQHATQLIALLAGVPGKVNLIPFNPFPGARFQRSPHEAIRRFSDYLNSAGIVTTTRRTRGDDIDAACGQLAGQVRDRTRRIIRMEAAG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for [4Fe-4S] cluster binding.2. The protein design should emphasize Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "FQSYVPIMNGCNNFCTYCIVPYVRGREVSRPVNEILQEITELSSRGVREITLLGQNVNSYKGEDGEGRLIDFPKLLTLIAREADKTDMIRWIRFMSSHPKDMSDALIDTIAAEKRLCKLVHLPVQHGSDTILKRMNRVYTIEHYKNRIKRLKETIPDIALSTDILMGFPGETEDDVKATLDLMQEIEFDSAFMYHYNPREGTKAFNYPDRIPEEVKIERLGRVIS",
    "response#2": "SSQAGCRMGCAFCASGINGLARDLTAGEMVSQVLEVERYTGEPISRIVVMGTGEPFDNYENLARFIEIMNDPGGRKTGMRNLTVSTCGIIPAILKFAKDFPQVNLAISLHAPIDELRSGMMPVNRSYPVGDLMAAAAEYTEMTHRRITFEYALVSGVNDD",
    "response#3": "SSQVGCAMGCRFCASTLGGLVRNLTAAEMAGQLLVVNRDLAERGQRVSHVVVMGIGEPLQNLDATLQFLRVAHHPQGAGISYRHMTVSTSGLVPRIRQLAHAGLPITLAVSLHAPNDALRSWLMPVNRRWPIAELMDACREYVERTGRRITFEYVLIEDVNDRPEHAAEY"
  },
  {
    "reference": "MTAAPAMSWWQVIVLAAAQGLTEFLPVSSSGHLAIVSRIFFSGDAGASFTAVSQLGTEAAVVIYFARDIVRILSAWLHGLVVKAHRNTDYRLGWYVIIGTIPICILGLFFKDDIRSGVRNLWVVVTALVVFSGVIALAEYVGRQSRHIERLTWRDAVVVGIAQTLALVPGVSRSGSTISAGLFLGLDRELAARFGFLLAIPAVFASGLFSLPDAFHPVTEGMSATGPQLLVATLIAFVLGLTAVAWLLRFLVRHNMYWFVGYRVLVGTGMLVLLATGTVAAT",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. A protein that contributes to regulation of cell shape, dephosphorylation, cell wall organization, peptidoglycan biosynthetic process, response to antibiotic by functioning in plasma membrane with undecaprenyl-diphosphatase activity.3. We are looking for a protein with broad substrate specificity for di-trans,octa-cis-undecaprenyl diphosphate + H2O = di-   trans,octa-cis-undecaprenyl phosphate + H(+) + phosphate.4. The protein must exhibit the following characteristics: Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP); Confers resistance to bacitracin.. The designed protein sequence is ",
    "response#1": "MICLLCCL",
    "response#2": "MSPRDPASR",
    "response#3": "LVWLD"
  },
  {
    "reference": "MPQLNPNPWFTILIFTWAVFLTILPNKVTSHKMPNELLTKDPSNLLTEIWYWPWH",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation; Part of the complex F(0) domain2. The protein should be optimized for stability and solubility when expressed in mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) where it will be involved in proton motive force-driven ATP synthesis.3. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "MGSIGASIATLGLGGAAIGIALVFVALINGTSRNPSLRSTLFPQAILGFALSEACGLFCLMISFLL",
    "response#2": "MFIGASIATLGLGGAAIGIALVFVALINGTSRNPSLRSTLFPQAILGFALSEACGLFCLMISFLLLY",
    "response#3": "IGASIATLGLGGAAIGIALVFVALINGTSRNPSLRSTLFPQAILGFALSEACGLFCLMISFLL"
  },
  {
    "reference": "MLIIKESDVKKLISLKEVIDINEQVFIKESKEEVVCPERIILPVEKTQPQQDSNEPINKKQLVGNLYFKPSLVVDESVGIKIVGTFANNANKGLPTVPATIILNDIETGLANAVIGATYITGARTAAGSAISVKYLATESPETIFVFGSSLQAQLHIEMILLLKPTIKKVYISSRSKENVDKLIIQLKNENNYNNDAVEFNYCAATGAGDDKDIINRYLLEADIIVTATSSNEPLFNGHEVLSEKQRDFKKKPLLICAVGSSRPTNRELDSFTISNSNLIVDDVNSCMVSGELFIPINKENIITKSHILGKLSDVVSGKYKQQTTTTTSKSITVYKSSGTAIQDVATANYIYKKALQNNIGYNININD",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein can be targeted to cytoplasm and perform lyase activity. The designed protein sequence is ",
    "response#1": "PPCQEVVIDGENLDLNQIPLIRPYPGDAGKIITLGLVITKDCETGTPNVGVYRLQLQSKTTMTVHWLSVRGGARHLRKAAEQGKKLEVAIALGVDPLIIMAAATPIPVDLSEWLFAGLYGGSGVALAKCKTVDLEVPADSEFVLEGTITPGEMLPDGPFGDHMGYYGGVEDSPLVRFQCLTHRKNPVYLTTFSGRY",
    "response#2": "PPCQEVVIDGENLDLNQIPLIRPYPGDAGKIITLGLVITKDCETGTPNVGVYRLQLQSKTTMTVHWLSVRGGARHLRKAAEQGKKLEVAIALGVDPLIIMAAATPIPVDLSEWLFAGLYGGSGVALAKCKTVDLEVPADSEFVLEGTITPGEMLPDGPFGDHMGYYGGVEDSPLVRFQCLTHRKNPVYLTTFSGRY",
    "response#3": "PPCQEVVIDGENLDLNQIPLIRPYPGDAGKIITLGLVITKDCETGTPNVGVYRLQLQSKTTMTVHWLSVRGGARHLRKAAEQGKKLEVAIALGVDPLIIMAAATPIPVDLSEWLFAGLYGGSGVALAKCKTVDLEVPADSEFVLEGTITPGEMLPDGPFGDHMGYYGGVEDSPLVRFQCLTHRKNPVYLTTFSGRY"
  },
  {
    "reference": "MAKYIFVTGGVLSSVGKGVTAASIGKMLQARGFKVTAIKIDPYVNVDAGTMNPYMHGEVFVTDDGGETDLDLGWYERFLDVNMSKDHYITTGQVYSTVIERERRGDYLGKCVQIIPHITDEIKRRIRRVAEKTGAEVVITEIGGTTGDIEGLPFLEAARQMRLEEGFTNTLFVHVALVPILETTGEQKTKPLQHSVNELRRIGIQPDLVVARCSVMIEAEARRKIALFANVEEKAVFCSPTVKNIYGIPLLLDGQGMGDYICRRLGLQASKPDWSWWNRILEGFNQPRHEVRVVFCGKYAKLADSYISINEALKHAGAQLYTAVKIDWIEAETFEEDPGKLEILNAYDGLLVPPGFGSRGAEGKILAVKYARERGIPFLGICFGFQMAVVEFARHVCGLKDANSTEVNPETPHPVLDYMPEQKGVERLGGTMRLGAQPVKLKPGTLARKLYGSEIIYQRHRHRFEVNPAYWEILQEKGLVFSGVSPDGRRVEIFEYPGSLFHMGVQYHPEFKSRPGKPDPAYVGFIEACLKRRLQGRL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must have at least one CTP synthase N-terminal domain.2. The Nucleophile; for glutamine hydrolysis, Nucleophile active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. The designed protein should be optimized for CTP synthase activity, metal ion binding activity in order to improve glutamine metabolic process.4. A protein that is capable of catalyzing reactions within the Pyrimidine metabolism pathway with high efficiency and specificity.5. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.. The designed protein sequence is ",
    "response#1": "VTIAMVGKYIGLRDSYISITEALKHAAAVLGCGIQIKWVSSDNISYDMVGEQVAEADGILVPYGFGSRGAEGKINVIRYARENRVPFLGICFGFQLATIEFARNVLGIRNANSVEIDPDTPNPVIDLLPEQKLVKEKGGSMRLGAHPIKLEPGTIAYKIYGGNYRISERHRHRYEVNPDYIQQFEKHGLKFSGRSLDGRRMEILEIKDHPCFIATQFHPEYKSRPGKPSPIFLHFIKDHMK",
    "response#2": "LADSYVSILEALKHAGGVLSTKVEIDLIEAEIFEDEPEKLSMLDSYDGIIVPPGFGRRGTEGKIAVANYAREMDIPFLGLCFGFQMAIVAFARYVCKLDGANSTENDPNTSHPVIDLMLEQRNVERMGGSMRLGAYPIRIKPGTLLYNLYSQNIVYERHRHRWEVNPEYWGILESHGMVFSGVSLDGDRIEAFEIPSLKFYVGTQYHPEFKSRPGKPSPIFYGFVES",
    "response#3": "LQDSYKSILESFIHAGAENEIKVKVVSIHSEHLDGTNVAEKFKGLHGILVAPGFGERGIEGKIDAVKYAREHKIPYFGICLGMQMAVIEYSRNVLGLKGANSTEMNKETPYPVIGLMDEQKHITDKGGTMRLGAWKCDLVASSIASNTYVAKSIEERHRHRYEYNNEFREQLEAAGLKTTGINPETGLVEIVEIENHPWFVGVQYHPEYKSTVVNPHPLFVSFXIKA"
  },
  {
    "reference": "MSQPFALHHDGENQMQRRGKMNTRSNGLSGQKRAALGVITNQVNQQVRIQPSRAAKPKSSEFNIQDENAFTKKNAKTFGQQPSQFSVFVDPTPAAPVQKAPTSHVTDIPAALTTLQRVPLTEVPGSPDIISLEDSMESPMILDLPEEEKPLDREAVILTVPEYEEDIYNYLRQAEMKNRAKPGYMKRQTDITTSMRCILVDWLVEVSEEDKLHRETLFLGVNYIDRFLSKISVLRGKLQLVGAASMFLAAKYEEIYPPDVKEFAYITDDTYTSQQVLRMEHLILKVLTFDVAVPTTNWFCEDFLKSCDADDKLKSLTMFLTELTLIDMDAYLKYLPSITAAAALCLARYSLGIEPWPQNLVKKTGYEIGHFVDCLKDLHKTSLGAESHQQQAVQEKYKQDKYHQVSDFSKNPVPHNLALLAL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in mitotic cell cycle phase transition, cell division, potentially with improved properties such as stability, specificity or selectivity.2. The protein must exhibit the following characteristics: G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis.; Essential for the control of the cell cycle at the G2/M (mitosis) transition; Interacts with the CDC2 and CDK2 protein kinases to form MPF. The designed protein sequence is ",
    "response#1": "PTAYVFMRRFLKAAQADKKLELVAFFLVDLSLVEYEMLKFPPSLVAAAAVYTAQCTVSGFKHWNKTCEWHTNYSEDQLLECSMLMVGFHQKAGAGKLTGVHRKYGSAKFSFTAKCEC",
    "response#2": "VFMRRFLKAAQADKKLELVAFFLVDLSLVEYEMLKFPPSLVAAAAVYTAQCTVSGFKHWNKTCEWHTNYSEDQLLECSMLMVA",
    "response#3": "PLCLHFLRRNSKAGAVDAQKHTLAKYLMELTLVEYEFITKLPSEIAAAALYLALKLIDDSNWTPTLAHYSGYTEDEILSTVSKLSILTLSMDNSKYQAVKNKYSASKFLRISLIPS"
  },
  {
    "reference": "MELSSGKLARFADGAAVVQLGDTAVMVTAVSKTKPSASQFMPLVVDYRQKAAAAGRIPTNYLRRELGTTDKEILTSRVIDRSIRPLFPVGYFYDTQILCNLLAVDGVNDPDVLAINGASAALALSDIPWNGPVGAVRVGLVDGETVINPTRKQMSSSTLNLVVAAAPQNQVVMLEATAENILQQDFCHAIKVGVKQTQQIIQGIQQLVKERGVVKRNVQKLFVAPEEIVECAKKLAFNKIYAVFTDFSHDKISRDEAINKIRLETEEQLKEKFPEAEPYEIMESFNAVSKDIFRNLILNEYRRCDGRDLTSLRDIKCEVNMFKMLHGSALFQRGQTQVLCTVTFDSIESSVKSDLISTAVSGIKDKNFMLHYEFPPYATNEIGKVTGVNRRELGHGALAERALKPVIPQDFPFTIRVTSEVLESNGSSSMASACGGSLALMDAGVPVSSAVAGVAVGLVTKYDLEGRDLEDYRLLTDILGIEDYYGDMDFKMAGTSKGVTALQVDLKLPGIPIKIIMEAIQQATAAKREILQIMNETLAKPRPNRKESGPVVETIHVPLSKRLRFLGPGAYNLKKLQAKTGVTLSQLDEETYSVFAPTPSAMHEAREFIGELCKDDQEVNLEFGAVYTATITEIRDSGVMVKLYPNMTPVLLHNSQLDQRKIKHPSALGLEVGQEIQVKYFGRDPTDGRMRLSRKVLQSPATTVLKTLTDKNSIVLGGTAPESSTSSQ",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be involved in rRNA import into mitochondrion, mitochondrial mRNA polyadenylation, mitochondrion morphogenesis, mitochondrial RNA 5'-end processing by localizing to mitochondrial degradosome, cytosol, mitochondrial intermembrane space and exhibiting miRNA binding, poly(U) RNA binding, 3'-5'-exoribonuclease activity, identical protein binding.2. The designed protein have S1 motif domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "IQKSGQCSGCGRTIEPIHLSPEEYEFLKEKIMRDVIDGGDQYKKTTPQELKRFESFVNSCPPFDIVIDGLNVAKMFPKGRESQNLLGVVSQLAQQNLQLLVLGRKHMLRPSSQWRKEEMEQVRKQAHCFFADNISEDDPFLLYATLNSGNHCKFITKDLLRDHKACLPDARTQRLFFKWQQGHQLAIMKGFQKSKLTFQHILSYDTVVQRTGDSWHIPYDEDLVQRSSCEVPTKWLK",
    "response#2": "IQKSGQCSGCGRTIEPIHLSPEEYEFLKEKIMRDVIDGGDQYKKTTPQELKRFESFVNSCPPFDIVIDGLNVAKMFPKGRESQNLLGVVSQLAQQNLQLLVLGRKHMLRPSSQWRKEEMEQVRKQAHCFFADNISEDDPFLLYATLNSGNHCKFITKDLLRDHKACLPDARTQRLFFKWQQGHQLAIMKGFQKSKLTFQHILSYDTVVQRTGDSWHIPYDEDLVQRSSCEVPTKWLR",
    "response#3": "YVQPPYTLVLEIKDVLVHPDWTYETGWRFKKRPGVDVFLKECAKYFEIVVYTAEQGVTVFPLVDALDPNGCIMYRLVRDSTHFDGGHHVKNLDNLNRDLKRVVVVDWDRNSTKFHPSNSFSIPRWSGNDNDTTLFELTSFLSVL"
  },
  {
    "reference": "DDFLCAGGCL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be able to regulate killing of cells of another organism, defense response to fungus.2. For general function, the protein need meet that Lacks mitogenic towards murine splenocytes; Has antiproliferative activity towards L1210 leukemia cells with an IC(50) of 8.33 uM, but lacks antiproliferative activity towards HepG2 hepatoma cells; Has antibacterial activity against the Gram-negative bacterium P.fluorescens; Lacks ribonuclease activity towards yeast tRNA and protease activity towards casein.; Has antifungal activity against M.arachidicola with an IC(50) of 1.3 uM, but not against F.oxysporum The designed protein sequence is ",
    "response#1": "CRCRIGRCLGLEVYFGVCFLHGRLARRCL",
    "response#2": "CRCRIGRCLGLEVYFGVCFLHGRLARRCG",
    "response#3": "CRCRIGRCLGLEVYFGVCFLHGRLARRCL"
  },
  {
    "reference": "MWRETKLLLIDDNLDRSRDLAVILNFLGEDQLTCNSEDWREVAAGLSNSREALCVLLGSVESKGGAVELLKQLASWDEYLPILLIGEPAPADWPEELRRRVLASLEMPPSYNKLLDSLHRAQVYREMYDQARERGRSREPNLFRSLVGTSRAIQQVRQMMQQVADTDASVLILGESGTGKEVVARNLHYHSKRREGPFVPVNCGAIPAELLESELFGHEKGAFTGAITSRAGRFELANGGTLFLDEIGDMPLPMQVKLLRVLQERTFERVGSNKTQNVDVRIIAATHKNLEKMIEDGTFREDLYYRLNVFPIEMAPLRERVEDIALLLNELISRMEHEKRGSIRFNSAAIMSLCRHDWPGNVRELANLVERLAIMHPYGVIGVGELPKKFRHVDDEDEQLASSLREELEERAAINAGLPGMDAPAMLPAEGLDLKDYLANLEQGLIQQALDDAGGVVARAAERLRIRRTTLVEKMRKYGMSRRDDDLSDD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: AAA+ ATPase enhancer-binding protein that acts as a transcription regulator and plays a role in the modulation of mucin adhesion and flagellar gene expression; In addition to flagella genes, regulates also expression of biofilm-related genes; Functions as a transcriptional repressor in the absence of c-di-GMP and as an activator when c-di-GMP is present..2. The design objective is to achieve a protein with strand-helix-strand-turn-helix-strand-helix-strand-helix-strand-helix-strand-helix-helix-strand characteristics.3. The ideal protein would be one that plays a critical role in positive regulation of cell-substrate adhesion, negative regulation of extracellular matrix assembly, regulation of DNA-templated transcription through ATP binding, transcription cis-regulatory region binding, DNA-binding transcription activator activity within protein-DNA complex.4. The designed protein should have a high specificity for 3',3'-c-di-GMP, ADP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "GMALVGTEGQWLQTNKALCQFLGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALLAVSLVRHTDGTPLYFIAQIEDINFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRITVKDGIRHIRALANRVLNKEGEVERLLGIEKERLHITLDSIGEAVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTSRSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLYAMTSPLEIGQHLKRIISANVLVTVFSNHGKSFILTKLLAIDLKSGRFAFDAGSSDDANRQLLKSERNVFVCTPDGIKTQFVTGPVQSFIYDGAPAFLARLPPEVHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALLRELASLMLSMLRSSDVLARLGGDEFGLDDSDRAMSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSI",
    "response#2": "MGVQFGDFIPKNIISFEDLKGKKVAIDGMNALYQFLTSIRLRDGSPLRNRKGEITSAYNGVFYKTIHLLENDITPIWVFDGEPPKLKEKTRKVRREMKEKENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSYSASHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALLRELASLMLSMLRSSDVLARLGGDEFNNHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRIGEAVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTSRSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDTSSH",
    "response#3": "GIWEWELKPNIFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRITVKDGIRHIRALANRVLNKEGEVERLLGINMDEKERLHITLDSIGEAVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTSRSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLGGVKSSKASPSSTLAAVYYENFIKPTCSHIINNGGLLDKNGYIYKKCTIKIIIPKKLTSDVNSQFQRIKAKIETKELSFEYLGRPRNINVEIIAEDGEVMIIDFPTILSGINYAISNLLPQDFNSMSVDYEAILSRELERFVYTLGKLEFRITVKDGIRHIRALANRVLNKEGEVERLLGINMDMTEGPSSTLAAVYYENFIKPTCSHIINNGGLLDKNGYIYKKCTIKIIIPKKLTSDVNSQFQRIKAKIETKELSFEYLGRPRNINVEIIAEDGEVMIIDFPTILSGINYAISNLLPQDFNSMSVDYEAILSRELERFVYTLGEK"
  },
  {
    "reference": "MNSTEITQNTDVPSHFRSIKTFVLRTGRMTEGQKRNYEAFYQKWCIPYTESPIDFQTLFQNDNPVIIEIGFGMGTATAQIAEDNPDKNYLGIEVFKAGVGKLLGEIEEKKLNNLRIIEHDAIEVLENMINNESVDGFHIFFPDPWQKKRNHKRRLVRRPRTDLLSKKLKDGGYIYMVTDWENYAEDAFEQLSETPNLKSKYEKFAPSQSWRPTTKFEKKGLKKEHVINELIFEKD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein's active site must be carefully engineered to ensure that the amino acids involved in guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-   methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine are properly positioned for optimal catalysis.2. For general function, the protein need meet that Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.3. A protein that can modulate the overall flux of metabolites through the tRNA modification pathway, enhancing or suppressing its activity as needed.4. The protein must be able to tRNA (guanine-N7-)-methyltransferase activity. The designed protein sequence is ",
    "response#1": "MFSKIGHIIHLNLKNHLMPYRRLIGEVLMDKVADCRTVVNKSNSIQNTYRNFEMELICGVPEYEVSIKENGCTYKFNFSRVYWNPRLSTEHQKITDMLEEGDLLYDLYAGVGPFTVPAAKRGCTVIANDLNPDSYSALVINCGLNKVMRNVKCYNMDAVDFIKVELRNDLLAKLADDKFQGNIHITMNLPAMAVEHLVHFPGLFSGESIELRIKPLVHVYCFAQGADDKKPIAQQKVEQWLGVEVTDMLKEITFVRNVAPNKDMMRVRNPSAV",
    "response#2": "FSKIGHIIHLNLKNHLMPYRRLIGEVLMDKVADCRTVVNKSNSIQNTYRNFEMELICGVPEYEVSIKENGCTYKFNFSRVYWNPRLSTEHQKITDMLEEGDLLYDLYAGVGPFTVPAAKRGCTVIANDLNPDSYSALVINCGLNKVMRNVKCYNMDAVDFIKVELRNDLLAKLADDKFQGNIHITMNLPAMAVEHLVHFPGLFSGESIELRIKPLVHVYCFAQGADDKKPIAQQKVEQWLGVEVTDMLKEITFVRNVAPNKDMMRVRNPSAW",
    "response#3": "MFSKIGHIIHLNLKNHLMPYRRLIGEVLMDKVADCRTVVNKSNSIQNTYRNFEMELICGVPEYEVSIKENGCTYKFNFSRVYWNPRLSTEHQKITDMLEEGDLLYDLYAGVGPFTVPAAKRGCTVIANDLNPDSYSALVINCGLNKVMRNVKCYNMDAVDFIKVELRNDLLAKLADDKFQGNIHITMNLPAMAVEHLVHFPGLFSGESIELRIKPLVHVYCFAQGADDKKPIAQQKVEQWLGVEVTDMLKEITFVRNVAPNKDMMRVRNPSARV"
  },
  {
    "reference": "MATATAAGCSGAVALKSLGGRRLCIPQQLSPVLAGFGSHAAKSFPILSTRSIATSGIRAQVATAEKVSAGAGQSVESPVVIVTGASRGIGKAIALSLGKAGCKVLVNYARSSKEAEEVSKEIEAFGGQALTFGGDVSKEEDVEAMIKTAVDAWGTVDILVNNAGITRDGLLMRMKKSQWQEVIDLNLTGVFLCTQAAAKIMMKKKKGRIINIASVVGLVGNAGQANYSAAKAGVIGFTKTVAREYASRNINVNAVAPGFISSDMTSKLGDDINKKILETIPLGRYGQPEEVAGLVEFLAINPASSYVTGQVFTIDGGMTM",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that contributes to fatty acid biosynthetic process by functioning in chloroplast with NAD binding.2. The protein should be optimized for activity in fatty acid biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The protein should be designed to catalyze the following reaction a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+)   + NADPH. The designed protein sequence is ",
    "response#1": "MYPNRISYWFDSKSPSYYVDCGCVGSMAVVHLAFNSIVAGECEAALVGGCSLCLSSTVTLNLINAGLLCNDGKTKCFDKRADGYVRSETIGFLFLQKAKDAKRIYAEIYHTKLGYCSSTLNNGQIVRHSNSKDIIKFLKKFYSEIDVSPQEVEYVEANGIANAEDDKNELEAIGEIFGNKERAVKIGCVKSNMGNSESASGLCALTKVCLAYRNGELSGNLHYENPHDDVDAVRDGRIEVVHENTPFKRRFAAVNSFSYTGPMAHVLLKAR",
    "response#2": "MYPNRISYWFDSKSPSYYVDCGCVGSMAVVHLAFNSIVAGECEAALVGGCSLCLSSTVTLNLINAGLLCNDGKTKCFDKRADGYVRSETIGFLFLQKAKDAKRIYAEIYHTKLGYCSSTLNNGQIVRHSNSKDIIKFLKKFYSEIDVSPQEVEYVEANGIANAEDDKNELEAIGEIFGNKERAVKIGCVKSNMGNSESASGLCALTKVCLAYRNGELSGNLHYENPHDDVDAVRDGRIEVVHENTPFKRRFAAVNSFSYTGPMAHVLLKYPNRISYWFDSKSPSYYVDCGCVGSMAVVHLAFNSIVAGECEAALVGGCSLCLSSTVTLNLINAGLLCNDGKTKCFDKRADGYVRSETIGFLFLQKAKDAKRIYAEIYHTKLGYCSSTLNNGQIVRHSNSKDIIKFLKKFYSEIDVSPQEVEYVEANGIANAEDDKNELEAIGEIFGNKERAVKIGCVKSNMGNSESASGLCALTKVCLAYRNGELSGNLHYENPHDDVDAVRDGRIEVVHENTPFKRRFAAVNSFSYTGPMAHVLLKGHYR",
    "response#3": "YPNRISYWFDSKSPSYYVDCGCVGSMAVVHLAFNSIVAGECEAALVGGCSLCLSSTVTLNLINAGLLCNDGKTKCFDKRADGYVRSETIGFLFLQKAKDAKRIYAEIYHTKLGYCSSTLNNGQIVRHSNSKDIIKFLKKFYSEIDVSPQEVEYVEANGIANAEDDKNELEAIGEIFGNKERAVKIGCVKSNMGNSESASGLCALTKVCLAYRNGELSGNLHYENPHDDVDAVRDGRIEVVHENTPFKRRFAAVNSFSYTGPMAHVLLKGHYK"
  },
  {
    "reference": "MERVWFAKLTNKGTIKIGYISFILLSLLCQSLIGLVNADFNYEGCYSAADIQSAGLSLKNSYIYQSVSYCQNQCPESAVVALFNGSDCYCGNSVSFLTSLTKSTDSNCGTKCSGWPYQMCGGSSYMNVYVNAETFVSSVESSSSKEGSSTSYMPSTTSSLSSAQISSTTRRTSTDMKSSEMIATTVSTTSTTSSSTSSTTSSTTSSTTSSTTSSTTSSSTSSTTSSTTSSTTSSTTSSTTSSTTSSTTSSTTSSTTSIFSVTSSSSSITLSSSEHTTVDSRTSSPSSTLVPVSSSSSTLSTPKVTSMTPSTSSTIPIVTSVELVTSVVTKAIVSTSDQHQETIFVTRTSVVERSSEVATATAAASNNRSNSTSKQRLSGGAIAGIVIGVVFGVIFIILILLFLIWRRRKSHDQLDLEETKHYQPYSFGDEDANPIGPPPSSGTTNWMRHSRGNTAGSIGTSNMYGFSMSNGANYSSPSSNTSGSIINNLAGLQDATVQKQNLPSTVFEEANTLNSANERFSANSLPDMMMSGPLQVVNPDNPDNPELSSTVSHNRA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The Cytoplasmic, Extracellular topological domain of the protein should be designed to be flexible or rigid.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.4. The designed protein have WSC domains with specific sequence motifs that are crucial for its activity.5. The designed protein should have transmembrane signaling receptor activity within fungal-type vacuole, mating projection tip to regulate response to heat, Rho protein signal transduction. The designed protein sequence is ",
    "response#1": "KDAVYFEVKVYNHKPNSNSIFSIGLVTVPYPYFRIPGMCKFSIAYESTGKLRINDPFFPSTLLPKLVEGDVVGFGYRFKTGTIFITHNGKKLMDVTQNVSVELFIALGAMNASYTRTYTKDGLLQ",
    "response#2": "DLQMIKDRGIQSYYFIPSINDNVDKSGHFLPSFLVQDKLEVEFTRWNKSSSAVLNYPLPYNKKDAVYFEVKVYNHKPNSNSIFSIGLVTVPYPYFRIPGMCKFSIAYESTGKLRINDPFFPSTLLPKLVEGDVVGFGYRFKTGTIFITHNGKKLMDVTQNVSVELFIALGAMNASYTRTYTKDGLLEP",
    "response#3": "KSKNGEEWPLVFSDEFNAEGRTFYEGDDQFFTAPDLHYDATKDLEWYDPDAVTTANGTLTLRMDAFKNHGLFYRSGMIQSWNQMCFTQGKLEFSAKLPGYGNITGFWPGLWSMGNLGRPGYLASTEGVWPYTYDSCDAGITPNQSSPDGISYLPGQRLNKCTCPGEAHPNRGVGRGAPEIDALEGEIHGVIGRVSQSLQVAPYDIWYMPNYDFLEIHNSSITLMNTYAGGPFQQAISGVTMLNVTWYEYGDHQHNFQKYGYEYLNDDESGYLRWFVGDNPTFTIYSQALHPNGNVGWRKLPKEPLSLILNFGISNNWAYIDWPSLVFPSTMRVDYVRVYQPKDS"
  },
  {
    "reference": "MAHLIEAAPNPHQPFVVTAGVAVGAAMRELELPNKGPEAIVCVKDSEGTLRDLSFVPEQDSKFTPVPANTEDGRTVIRHSCTHVLAQAVQAEFPGTKLGIGPAIENGFYYDFDVAEPFTPEDLQKLEKRMKKIVKQGQKFERRIFASVAEAEEALKNEPYKLELIRDKGNVDPGSDEATEIGAGDLTGYYNVNPRTGEVDWYDLCRGPHVPTTKYIPAFALTRSSAAYWRGDQSLAGLQRIYGTAWESKEALEEYQLMMAEAEKRDHRRLGAELDLFSFPDEIGSGFPVFHPNGGIVRLEMEEHSRRRHINAGYSFVNTPHITKGDLFKKSGHLDFYADGMFPPMQLDGEVDEEGNVVKQAQDYYAKPMNCPMHNLIFASRGRSYRELPLRLFEFGTVYRYEKSGVIHGLTRARGFTQDDAHIYCTEDQLEQELTSVLDFIISLLRDYGLDDFYLELSTKDPNKFVGSDEIWEKSTSILQRVATKSGLELVPDPAGAAFYGPKISVQARDAIGRTWQMSTVQLDFNLPERFNLEYTSSDGSKKRPIMIHRALFGSIERFFGVLLEHYAGAFPAWLAPHQVIGIPVADSFSEHLEKVTGMLRDNGVRASVDTSDDRMQKKIRNHTTGKVPFMLLAGARDVEANAVSFRFLDGTQINGVPVDKAIGVITQWISSRNNKQPSEETVKKLV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that can perform threonine-tRNA ligase activity, tRNA binding in cytoplasm to support threonyl-tRNA aminoacylation.2. The protein should have a low Km value for ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-   threonyl-tRNA(Thr). The designed protein sequence is ",
    "response#1": "MDNETLVLRPMNCPHHMMIYKQDIHSYRELPIRIAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKDEFIRTVRLIQDVYEDFGLSDYTFRLSYRDPEDTEKYFDDDEMWNKAQSMLKEAMDEIGHDYYEAEGEAAFYGPKLDVQVKTAIGKEETLSTVQLDFLLPERFDLTYIGEDGKQHRPVVIHRGVVSTMERFVAFLIEER",
    "response#2": "DHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLERYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNCPHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQIQEEFQRALQLIIDVYADFNLTDYRFRLSYRDPNDTHKYYDNDEMWENAQSMLKAALDEMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEEHRPVMIHRGVISTMERFTAILIETYKGAFPTWLAY",
    "response#3": "DHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLERYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNCPHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQIQEEFQRALQLIIDVYADFNLTDYRFRLSYRDPNDTHKYYDNDEMWENAQSMLKAALDEMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEEHRPVMIHRGVISTMERFTAILIETYKGAFD"
  },
  {
    "reference": "MGGKWSKSSIVGWPQIRERLRQASPAAEGVGKVSQDLDKHGAVTISNINHPSNVWLEAQKEEDVGFPVRPQVPLRPMTYKAALDLSHFLKEKGGLEGLTYSRKRQDILDLWVYNTQGFFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPEEVERATEGENNSLLHPMCQHGMDDDERETLIWKFDSHLAFKHIAREMHPEFYKDC",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be optimized for SH3 domain binding activity in order to improve suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I.2. For general function, the protein need meet that Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis The designed protein sequence is ",
    "response#1": "GHHHHHH",
    "response#2": "MLLKG",
    "response#3": "GQVNRLSGYA"
  },
  {
    "reference": "MKLGVVILAAGQGTRMKSALPKVLHPLAGRPLLGHVIDAARGLQAERIAVVYGHGGSRVPEAFADAPVQWVEQAQQLGTGHAVEQALPAVAGMERVLVLYGDVPLTSRETLESLLRAAEQSPLALLTVELDDPAGYGRIVRDDDGRIVRIVEHRDATPAELAIAEINTGILVADGPSLERWIARLENGNAQGEFYLTDIVAMAVAEGAGVTSAPAADPHEVAGVNDRVQLAGLERWYQGQQARRLMRDGVTLRDPARFDLRGTLATGRDVEIDVNVVIAGDVTLGDRVRIGANTVIRDSRIGDDVQILENCVIEEAVVGEASRIGPFSRLRPETVLAGRVHVGNFVEIKKSEVAEGSKINHLSYIGDTSIGRSVNIGAGTITCNYDGAFKHRTVIGDDAFIGSDTQLVAPVKVGAGATIGAGSTITRDTPDGGLTLSRAKQTSIPGWQRPTKEKK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that contributes to regulation of cell shape, cell morphogenesis, peptidoglycan biosynthetic process, lipid A biosynthetic process by functioning in cytoplasm with magnesium ion binding, glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity.2. The Mg(2+), acetyl-CoA, UDP-N-acetyl-alpha-D-glucosamine binding site should be stable and able to withstand changes in environmental conditions.3. The protein have enhanced acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate. The designed protein sequence is ",
    "response#1": "LVLAAGLGTRMRSDRAKVLHDLDGRPLISHVCRTAAALAPDKIFVIVGHQSDDVREAVLKELDEDQAEFVWQQKQLGTGDAVNAAREFLSDTDSTLVVLSGDVPMIRHETLAALIQQHRGHRGRGAACTVLTVVLKDPSGYGRVIRDADGLFERIVEQKDATEQQREIREINAGIYCFESRKLFAALSRVKNDNAQGEYYLTDVPALLREDNEDVAILEADDETTIRSINEVNPSSKSGEYYMTDAGMFAFDGKRALDALEAIDAENEKGEYYLTDTVALAKAKGAGVSIVDPANTYLDMDVTIGRDTLVEPGCVITGATVIGEGCHIKPHCTIEDSRLGDETVIGPSAHLRPGTELGRGCRIGNFVEVKNSKLADGVKADHLAYVGDADVGEGSSFGCGAITVNYDWDQKHRTTVGRDVKVGCNANLVAPVTLADGAVVAAGSTITTSVPEQSLAVARAKREDMLLGWAKRFREDLAEAEAAFQARRRQEMMESGVTLIAPDTVFFSHDTRIAADVVVEPNVVFGPGVNVASNVRIKAFSHLEGASLGEGAEAGPFARLRPGAELGANAKVGNFVEVKKARLGEGAKASHLTYLGDTDVGAHANIGAGTITCNYDGFFKHRTVIGEGAFIGSNTSLVAPVTIGAGAMTGSGSVITEDVPDNALALSRARTIVKGVPAKAKK",
    "response#2": "LVLAAGLGTRMRSDRAKVLHDLDGRPLISHVCRTAAALAPDKIFVIVGHQSDDVREAVLKELDEDQAEFVWQQKQLGTGDAVNAAREFLSDTDSTLVVLSGDVPMIRHETLAALIQQHRGHRGRGAACTVLTVVLKDPSGYGRVIRDADGLFERIVEQKDATEQQREIREINAGIYCFESRKLFAALSRVKNDNAQGEYYLTDVPALLREDNEDVAIRKMIETPGGEEILREMNTVNTWVYRADWLWDALSEVPVNPKSGEYYITDLVAIAVERGYRVAAYRHPEYEVLGVNSRGELARAEALYQRRLRERLMLSGVTLVDPARIDVRGRIEHGRDVFIDANVLFEGEVKLGDRVRIGPNVVVRNCEIGADTVIHPNCVLEQSVIGKDCSIGPFARMRPNNRLADGVHIGNWVELKNSQVGEGSKANHLTYLGDTTVGSKVNVGAGTVTCNYDGANKHRTLIGDGAFIGSGAMLVAPVTIGERATIGAGSTITKDAAAGKLTIARSRQVNKEG",
    "response#3": "LVLAAGLGTRMRSDRAKVLHDLDGRPLISHVCRTAAALAPDKIFVIVGHQSDDVREAVLKELDEDQAEFVWQQKQLGTGDAVNAAREFLSDTDSTLVVLSGDVPMIRHETLAALIQQHRGHRGRGAACTVLTVVLKDPSGYGRVIRDADGLFERIVEQKDATEQQREIREINAGIYCFESRKLFAALSRVKNDNAQGEYYLTDVPALLREDNEDVAIREIAGDRGETRITEINTGIYAFDAAVLREGLDRISADNAQGELYLTDVLSIARRDGRAVGAFELDDVIQTEGANDRVQLSVLGAELNRRIVRGWMLDGVTLVDPARIDVRGRIEHGRDVFIDANVLFEGEVKLGDRVRIGPNVVVRNCEIGADTVIHPNCVLEQSVIGKDCSIGPFARMRPNNRLADGVHIGNWVELKNSQVGEGSKANHLTYLGDTTVGSKVNVGAGTVTCNYDGANKHRTLIGDGAFIGSGAMLVAPVTIGERATIGAGSTITKDAAAGKLTIARSRQRNIEGWVERRRPGTPAAEAAERALAEQERAAEGDG"
  },
  {
    "reference": "MTERNTPPDETIDFGFREVRREEKAGMVRQVFDSVAPRYDLMNDLMSLGLHRAWKSAFAAAIGASPRESLLDLAGGTGDIAFRALEAGAGRVILSDINPSMLQVAQGRALDRGHAARLAFLCADAEHLPLPDRSVEKVSIAFGLRNCTDKPAVLREARRVLRPGGRFHCLEFSRVEVAALAPLYDAWSFQVLPRLGARIARDAESYQYLAESIRMFPDQESLAAMMREAGLERVAHRNLSGGIVAIHTGWRL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein should be able to manipulate the activity of enzymes in the menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2 pathway, either by activating or inhibiting their function.2. The protein must exhibit 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity. The designed protein sequence is ",
    "response#1": "LMTTLLP",
    "response#2": "LTAAPAF",
    "response#3": "MMARLDFD"
  },
  {
    "reference": "MQKQLQQLIDLLPEANVIGGADKTITDITADSRIVQPGSLFIALKGVHVDGHTFLSKAAEAGAAAALVEDVPSEIPEGLTLLQVKDVREAMEIVAPFFFDYPGKTMRMIGVTGTNGKTTSTNIIRQVLKSAGYKCGMIGTINVLIDEESVVSHNTTPDVVDLQKFLYRMKEAGCQYVVMEVSSHALALKRVAGIEYDTAVLTNITEDHLDFHKTMEAYRDAKSLLFEHLTDGEKPNKTAVFNADEPASKVIEARTKAPYLTYGMGKENDIFPIAYEVGAKGMHLCLHTPAGDMDLHLNITGKFNVYNVMCAVGALLAEKIDKSEIEKVLNGFAGVPGRFQLVEAGQPYTVIVDYAHTPDGLENVLNTARLITKGRLWAVFGCGGDRDNKKRPIMGGIALNLADDVVVTSDNPRSEDPEKIIDQIEVALKPVPEGKTVTRIADRKEAIHYALAHAGDNDVIMIAGKGHENYQILKDRTIHFDDKEVVEEYWKGKLA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The meso-2,6-diaminoheptanedioate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "VTGTNGKTTISQLVAQLTEQSGKASAIMGTAGNGRLGALIQSTHTTGDVLAVQRFVHEMGVDNIDILALEASSHGLDQYRLQGVPVQVAIYTNLSRDHLDYHSNMDDYARAKARLFNRNHFPTLTHAIINLDDEYATTMLESASNSGVQVWTYSMQTNKQATFVAQQIKPSLTGVEIEVVSDFGTVWLNSPLLGLFNVANLLAAVT",
    "response#2": "VTGTNGKTTTATLLYRMFSQLGHKCGLISTIAYYIDRDQVSATHTTPDALTINSLLDKMLDAGCTHCFMEVSSHSVVQERIAGLCFVGGIFTNLTHDHLDYHKTFAAYRDAKKRFFDMLPSGAFALTNQDDRNGLVMVQNCAARSYTYACRTMADFNARVLEHSVDGMQLSIDGIEVWTSFIGLHNAYNLLAVYACF",
    "response#3": "VTGTNGKTTVTAGIYSFLLDLDEKPALLGTRGFFALEERIEAKSMTTPSILHILNHMKTAKDRGCNYFIMEVSSHAIDQERIEGINFALKVHTNVTSDHLDYHKTIEEYRAVKSRFFSDESPKLLNKDDIKNITYNPMNAQSYGVDEPATFKVQAFSLKHGITAGIKHLSKEATFHSPMVGLFNLFNLMAAIGVTGTNGKTTTATLLYRMFSQLGHKCGLISTIAYYIDRDQVSATHTTPDALTINSLLDKMLDAGCTHCFMEVSSHSVVQERIAGLCFVGGIFTNLTHDHLDYHKTFAAYRDAKKRFFDMLPSGAFALTNQDDRNGLVMVQNCAARSYTYACRTMADFNARVLEHSVDGMQLSIDGIEVWTSFIGLHNAYNLLAVYACA"
  },
  {
    "reference": "MQTLLFVTGNVNKIKEVRTILGDRFTVSGKVEDITLAWNSSGMASNSSFENIGKLLEKSRCLNETSRSSGLLVTVPELILDMAPMRYGLFDKKFYKSGTNSETVLNIESERNVHSSSLSKLFADDDDRIRCMSCQCGSFYMREKFCSGNSSKSIGNENSYSLLSTLLDKEEVVSECSRKTSKVGEAPVWEPTSNLFRDTEERCPKNDSIFSGRKVHRVVFSPLLEKDPIFSHKNQVSTTSLAYRHVSRTSIFFDRFRYQRIPRAAIKNEDIDLPEYQGQPSEIARLKCLAASQRLQQPVIVEDTCLCFNALGGLPGPYIKWFLKNLKPDGLHKLLAGFEDKTAYAQCIFAYCENSSNPVVLFEGRTNGRIVQPRGETSFGWDSCFEPEGFSQTYAEMGSAIKNTISHRSKALAQLRNYFENKS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that The enzyme does not distinguish between the deoxy- and ribose forms; Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.; Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives2. The protein should be able to bind ITP, Mg(2+) ligand in a variety of conditions3. The ideal protein would be one that plays a critical role in nucleotide metabolic process through nucleoside triphosphate diphosphatase activity, nucleotide binding, metal ion binding within cytoplasm.4. The protein must bind to Mn(2+), Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MNNNNIIIATNNAHKVKELQAIVADKYNVKSLKDAGVVINPEETGNTFEENALIKAKAIHQETGLPAIADDSGLCVDALNGAPGIYSARYAGENATDKSNNEKLIKDLEGVPLDKRTAHFVTVIAVVFPDGKEEVVEGTMEGTITEHMRGKNGFGYDPLFYLEEKGKTMAELSMDEKNKISHRGIAIQKLAEKI",
    "response#2": "MNKILVASRNKGKVIEMEKILAPLSIDLVSMRDAGNFAEIPETGQTFAENAALKATEHARTIGLPAIADDSGLEVDYLNGAPGIYSARFAGEDASYSDNINKLLKELSGVPEAHRTARFRCVIAYVSPTGTHTIVEGTIEGFITTQPVGNGGFGYDPIFYVPSLNKSAAELNPAEKNAISHRGQALAKLIPILAQLI",
    "response#3": "MNKILIATNNQHKVLEFQDILKPFGFELLNLSDIGCNIDIPETGNTFIENAYIKAEHFSKSTKMDCIADDSGLEVDALNGAPGVYSARYSGTNASYSDNVSRLLKELDGIEDRSARFKTAIALIDSLGTYHFDGEIEGYILLKEQGDNGFGYDPLFIPDGYDKSFAEMSMEEKNAISHRGKALRKLIPYLKDVLEN"
  },
  {
    "reference": "MGKRLIIQRRGRGTPAHRVASHRFKDKIRYRSYDALEKEGSIKGIVTDIVHDPARTAPIAEVKFENGEKKFILAPESIQIDDEIECGISAPIKFGNTLPLAEIPEGTPIYDIENTPGDGGRFVRSSGTYASLITHDANQSVVELPSGELKYLNPRCRASIGVVAGGGRKEKPFLKAGNRWHAYKAKGKKFMTVRGVAMNAVDHPHGGGNRQHPGRPTTVSRHAPPGRKVGSIAAKRTGLKR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that One of the primary rRNA binding proteins; It has been suggested to have peptidyltransferase activity; this is somewhat controversial; Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation; Makes several contacts with the 16S rRNA in the 70S ribosome.2. The designed protein must be optimized for structural constituent of ribosome, rRNA binding. The designed protein sequence is ",
    "response#1": "KPGNVLPLGEVPEGIPVYNIETIPGDGGKLVRSGGCYAHVITHDVSKTIIKLPSGHMKTLNPMCRATIGVVAGGGRKEKPITKAGKKYHAMKAKAVLWPRVRGVAMNAVDHPFGGGNTQHAGKPTY",
    "response#2": "MGPIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTR",
    "response#3": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTS"
  },
  {
    "reference": "MTAPLATAPAAARSTVSEKPSLIGLSREGIGAELERIGVERKQIRMRTAQLWNWMYARGVTDFADMLNVSKDMRSTLESAFTLKRPEIVAEQVSEDGTRKWLMRFPARGAGRPVEIETVYIPEIGRGTLCVSSQVGCTLTCSFCHTGTQKLVRNLTAEEIVGQILVARDRLGDFPDATTPVGAAVPSEGRLVSNIVMMGMGEPLYNFDNVKEALLIASDGEGLSLSKRRITLSTSGVVPMIPRAGEEIGSMLAISLHAVRDDLRDLLVPLNKKYPIKELLDACRNYPGLSNARRITFEYVMLKDVNDSPADARELVRLLKGIPAKINLIPFNPWPGVNYQCSDWETIETFSDIVNRAGYASPIRTPRGRDILAACGQLKSESERMRARERLALEAMMAGDADE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be able to perform 4 iron, 4 sulfur cluster binding under specific conditions. The designed protein sequence is ",
    "response#1": "ICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYNPYVQARSRIDQLKRLGHSVDKVEFILMGGTFMSLPAEYRDFFIRNLHDALSGHTSANVEEAVAYSEHSATKCIGMTIETRPDYCLGPHLRQMLIYGCTRLEIGVQSTYEDVARDTNRGHTVAAVADCFCLAKDAGFKVVAHMMPDLPNVC",
    "response#2": "WENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKRLGGDPAKINPLVPVDLVIDHSVQVDVSRTVDALEQNQKIEFHRNHERFSFLKWGAQAFDGLFIAPPGSGIVHQVNLEYIAREVMNGTGNLLYPDSVVGTDSHTTMINGLGVCGWGVGGIEAEAVMLGQPMSMVLPEVVGYKFVGKLPDIATATDLVLTVTNELRKKGVVGKFVEFYGEGVSTLSVQDRATISNMAPEYGATMGFFPADENTIDYLASTGRSNTKIEYIKNYLSSQGLMCNYKSQSHPIFTTTMELDLSTVVPSLSGPKRPHDRISLNSMKQDFNSCLSSPVGFKGFGLTADQIQKKATFTFKDKQYTIGHGAVTIAAITSCTNTSNPSVMLGAGLLAKNAVEHGLEVAPYIKTSLSPGSGVVTEYFSHSGLQEPLNKLGFDLTGYGCMTCIGNSGELAEPLAEAITKEDLVVAGVLSGNRNFEGRIHPLLRANYLASPPLVVAYALAGTLKF",
    "response#3": "SQVGCPIGCTFCATGQGGFRRNLTAGEILLQVMALWHHLEGRGKGERISNVVFMGMGEPLLNCEAVLKARRILMHPLGWGIGHRRITISTCGIVPQIKRLARETPPPELAVSLHATTDAVRNKLVPVNRRYPLGELLGACREYTEVSGRRITFEYILIKGINDSV"
  },
  {
    "reference": "MIKIDLKDLDLEETKAYVEEIGLEAYRAEQIRQWIFGHKSTSFEGMTNISKELRSYLNSSCIISRLKITKTEISLDGTKKVLFELEDGNRIESVLIPERNHFTACISSQVGCAMGCKFCLTAKQGFIRNLRTSEIINQVIQIRELLDQPESLTNIVFMGMGEPLANFEAVKKAVNNIISPDALNFSRLRVTLSTCGLVPEIERLGKDFPINLAVSLNAGDDTTRNILMPINKKYPLKRLTETLKRFPLRKGRRITFEYILIRDINDRLSDATNLARLLKNIRAKINLIPFNRYNGSPFEKPDEKRILAFQDELIRKNYTVTIRKSKGADISAACGQLITAT",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein with rRNA (adenine-C2-)-methyltransferase activity is needed to enhance rRNA base methylation in a specific context.2. The protein must exhibit the following characteristics: m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity..3. [4Fe-4S] cluster is required for the proper folding and stability of the protein.4. The protein should be designed to have high stability and activity towards adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.5. The protein should be able to bind S-adenosyl-L-methionine ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "VEDRGAVCISSQVGCTLSCRFCHTGTQTLVRNLGAPEIVGQFMAARDSYGEWPSPKGETPRLLSTIVLMGMGEPLYNYANVAKAMKIVMDGEGIGLSRRRITLSTSGVVPMMDRAGAELGVNLAISLHAVRDDLRDELVPLNRKYPIKELLAACRRYPSASNARRITFEYIMLKGVNDSEAEARELVRLIAGLPAKVNLIPFNPWPGSRFEPSTKAALDRFAAIVNAAGFASPIPRAGGIEIMSDTRANLFDLSLAECEALAVEMGVKTFHGRNLFKWLHKHGITDFDAMTDLPKALRDRLRDTVRVELPRIVATHPSADGTIKWVMALHDGQRIETVCIPEGKRRTICVSSQVGCALECAFCATARQGFNRNLTVSEIIGQVWHAARELAQPPTNVVMMGMGEPLANFDAVVKAMDIMQDDLAYMLAKQRVTLSTSGIVPNIYRLRAVSDVSLAVSLHAPNDALRDQLVPINQKYPLAELIPACKAYIGHDKRRKITWEYVMLDGVNDSLIHAKQLIRLLEGVPSKLNLIPFNPFTGSDFCTSPMERIEAFRLRLTGAGIFAMTRKTRGDEIAAACGQLVGRVEDRTRARLARSALTS",
    "response#2": "MNLLDLTKSELEQLCVKHGQPKFRALQLTKWVHNKGEQDFAKMTDLQSSFRDYLSRELGFPLPEGVSKRISKDGTVKYLFPTTDGKKIETVYIPAGEKEKEGKDPVAEGREGKKKEGSSIVEDRGAVCISSQVGCTLSCRFCHTGTQTLVRNLGAPEIVGQFMAARDSYGEWPSPKGETPRLLSTIVLMGMGEPLYNYANVAKAMKIVMDGEGIGLSRRRITLSTSGVVPMMDRAGAELGVNLAISLHAVRDDLRDELVPLNRKYPIKELLAACRRYPSASNARRITFEYIMLKGVNDSEAEARELVRLIAGLPAKVNLIPFNPWPGSRFEPSTKAALDRFAAIVNAAGFASPIRTPRGRDILAACGQLKSASEKRAMPS",
    "response#3": "EDDRGTLCVSSQAGCAVGCRFCSTGHQGFSRNLRTAEIVAQLWFAEHHLRRRLQLPEGVRAITNVVMMGMGEPLQNYAALLPALRVMLDDHAYGLSRRRVTVSTSGVVPMIDRLRDDCPVALAVSLHAPHDALRDDLVPLNRKYPLAELLAACERYLAAAPRDFITFEYCMLDGVNDSLAHAAQLVDLVQGRGARAEPLPCKFNLIPFNPFPASGLVCSPRERVKAFAAVLQDAGIVTTIRKTRGDDIDGACGQLRASVEDRGAVCISSQVGCTLSCRFCHTGTQTLVRNLGAPEIVGQFMAARDSYGEWPSPKGETPRLLSTIVLMGMGEPLYNYANVAKAMKIVMDGEGIGLSRRRITLSTSGVVPMMDRAGAELGVNLAISLHAVRDDLRDELVPLNRKYPIKELLAACRRYPSASNARRITFEYIMLKGVNDSEAEARELVRLIAGLPAKVNLIPFNPWPGSRFEPSTKAALDRFAAIVNAAGFASPIRKPRGADLA"
  },
  {
    "reference": "MHKNKILVPLSNNSYEVIIKQGLINNIGEELIRIGINSNRKILIVSNKEISTLFGRKLLNNLKKNNFNAEIFNIKAGESHKNFASLSEIFNAAFEVGLDRNSLLIALGGGIVGDVTGFAAATWLRGIEYIQIPTTLLSMVDSSVGGKTAVNHPKGKNLIGAFYQPKAVFIDPETLITLPTREFKAGMAEVIKYGVIKDKSLFEYLENEKNRDKILNLENESLIKIINKSIKTKACIVSEDEKENGIRAILNYGHSFGHVIENLCGYGEYLHGEAISIGMKIAGDIATEKNLWSKEHSLRQDHLIESYGLPIQTPKIKKNDVMKILMGDKKVRNGKMRFILPIELGEVDIFNDINESQFLKYFN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for activity in chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7, Metabolic intermediate biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The protein design should aim to mimic or enhance the function of natural proteins involved in aromatic amino acid family biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.3. The protein requires Zn(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "GMAEVVKTAAIWNETEFTALEENAPLVLDTVRGRPSPSSSADLAPIRHILKRIVLASARVKAEVVSADEKEGGLRNLLNFGHSIGHAYEAILTPQVLHGEAVAIGMVKEAELARYLGVLRPSAVARLVKCIASYALPTSLADKRIVSLTAGKPCPVDVVLDKMAVDKKL",
    "response#2": "GMAEVVKTAAIWNETEFTALEENAPLVLDTVRGRPSPSSSADLAPIRHILKRIVLASARVKAEVVSADEKEGGLRNLLNFGHSIGHAYEAILTPQVLHGEAVAIGMVKEAELARYLGVLRPSAVARLVKCIASYALPTSLADKRIVSLTAGKPCPVDVVLDKMAVDKKL",
    "response#3": "GMAEVVKTAAIWNETEFTALEENAPLVLDTVRGRPSPSSSADLAPIRHILKRIVLASARVKAEVVSADEKEGGLRNLLNFGHSIGHAYEAILTPQVLHGEAVAIGMVKEAELARYLGVLRPSAVARLVKCIASYALPTSLADKRIVSLTAGKPCPVDVVLDKMAVDKKQ"
  },
  {
    "reference": "MRVMGIQRNCQQWWTWGILGFWMFLIYNVMGSLWVTVYYGVPVWKEANPTLFCASDAKAYETEVHNVWATHACVPTDPDPQELVLENVTENFNMWKNDMVEQMHQDIISLWDESLKPCVKLTPLCVTLNCSNYVANNRTSNATVAPKDIEMKNCSFNTTADLRDKRQTAHAVFSTLDITPLEANGSNYRITSCNTSTITQACPKVSFDPIPIHFCAPAGYAILKCNNKTFNGTGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEGDVIIRSENLTNNAKTIIVQLNETVQINCTRPNNNTRKSIGIGPGQTFYAAGAIIGDIRQAHCNISGEQWNKTLERVIGKLKEHFSNKTILFNQSSGGDPEITTHSFNCRGEFFYCNTTALFHNNITNSTIMLPCRIKQFINLWQGVGRAMYAPPIKGLITCNSSITGILLTRDGGDVNQTANETETFRPGGGDMRDNWRSELYKYKVVEIKPLGIAPTPAKRRVVEREKRAALGVMFLGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLALERYLRDQQLLGIWGCSGKLICTTAVPWNSSWSNRSQDEIWQNMTWMQWEREIDNYTDTIYRLLEVSQTQQETNEKDLLALDKWQNLWNWFGITKWLWYIKIFIMVVGGLIGLRIIFAVLSIINRVRKGYSPLSFQTLIPTPRGPDRPGGIEEEGGEQDRGRSIRLVSGFLALAWDDLRSLCLFSYHRLRDLLLIATRAVELL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must have at least one Retroviral envelope protein GP41-like domain.2. The protein should be optimized for stability and solubility when expressed in plasma membrane where it will be involved in fusion of virus membrane with host plasma membrane, viral protein processing, positive regulation of establishment of T cell polarity.3. The protein must contain a signal peptide for proper functionality.4. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.5. The protein should have Helical domains to ensure proper insertion into the membrane.6. The protein design must feature the YXXL motif; contains endocytosis signal motif.7. For general function, the protein need meet that The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Transmembrane protein gp41: Acts as a class I viral fusion protein; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain The designed protein sequence is ",
    "response#1": "MRVKGIKKNYQHLWRWGTMLLGMLMICSATEKLWVTIYYGVPVWKDAKTTLFCASDAKAYEKEVHNVWATHACVPTDPDPQELVLGNVTENFNMWKNDMVDQMNEDIISLWDQSLKPCVKLTPLCVTLNCSNDVTKNVTATSNDVTKNVTATSNSTDMKNCSFNATTEIRDKVKKEYALFYRLDIVPLKENNNFSYRLINCNTSTITQACPKVSFDPIPIHYCAPAGFAILKCNNKTFNGTGPCSNVSTVQCTHGIKPVVSTQLLLNGSLAEGDIIIRSENITNNAKTIIVHFNKSVEIECVRLNNNTRKSIRIGPGQAFYAHGDIIGDPRQAHCNISKTNWNKTLQEVSIKLREHFPNKTIKFNQSSGGDIEITTHSFNCGGEFFYCNTSSLFNSTYWPNGTYWPNDTERNSTNASITLQCRIKQIINMWQKVGRAMYAPPIEGIITCKSNITGLLLTRDGGRNETEETFRPGGGDMRDNWRSELYKYKVVEIKPLGIAPTKAKRRVVEREKRAVGIGALFLGFLGAAGSTMGAASITLTAQARQLLSGIVQQQSNLLRAIEVQQHMLQLTVWGIKQLQARVLAIERYLTDQQLLGIWGCSGKLICTTAVPWNSSWSNISQEDIWNNMTWMEWDRQISNYTGTIYRLLEDSQNQQEKNEKDLLALDSWKNLWSWFDITNWLWYIKIFIMIVGGLIGLRIIFAVIAIVNRVRQGYSPLSFQTLIPNPRGL",
    "response#2": "MRVKEIRKNYQHLWKWGIMLLGILMIYSATEQLWVTVYYGVPVWKEAKTTLFCASDAKAYDKEVHNVWATHACVPTDPNPQEMPLENVTEEFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLECRNVRNVSSNGTYNIIHNETYKEMKNCSFNATTVVEDRKQKVHALFYRLDIVPLDENNSSEKSSENSSEYYRLINCNTSAITQACPKVSFDPIPIHYCTPAGYAILKCNDNTFNGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIIRSENLTNNVKTIIVHLNQSVEIVCTRPNNNTRTSIRIGPGQTFYATGEIIGDIRQAHCNISEAKWNETLQRVGEKLAEHFPNKTINFTSSSGGDLEITTHSFNCRGEFFYCNTSGLFNGTYMPNGTNSNSSSNITLPCRIKQFINMWQGVGRAMYAPPIEGNITCNSIITGLLLERDGGETNSSTETFRPGGGDMRNNWRSELYRYKVVEIKPLGVAPTTAKRRVVEREKRAALGALFLGFLGAAGSTMGAASITLTAQARQLLSGIVQQQSNLLRAIEVQQHMLQLTVWGIKQLQARVLAIERYLTDQQLLGIWGCSGKLICTTAVPWNSSWSNISQEDIWNNMTWMEWDRQISNYTGTIYRLLEDSQNQQEKNEKDLLALDSWKNLWSWFDITNWLWYIKIFIMIVGGLIGLRIIFAVIAIVNRVRQGYSPLSFQT",
    "response#3": "MRVKGIRKNYQHLWRWGTMLLGMLMICSAVEQLWVTVYYGVPVWREAKTTLFCASDAKAYEQEVHNVWATHACVPTDPSPQEILLGNVTENFNMWKNDMVDQMHEDVISLWDQSLKPCVKLTPLCVTLNCSKVNITHNTTISISGTSNITNEMKNCSFNVTTEIKDRQKKVHALFYKLDIVPLGTENNTSSTSSYRLINCNTSAITQACPKVTFDPIPIHYCAPAGYAILRCNNNTFNGTGPCQNVSTVQCTHGIKPVVSTQLLLNGSLSEKGIMIRSANLTDNAKTIIVQLNDSVEITCTRPGNNTRQSIRIGPGQAFYATGDIIGNIREAHCNISGGKWNQTLKEVSEKLEKYFPNKTINFTSPVRGGDLEIMTHSFNCRGEFFYCNTSKLFSGVYNGTYNVTTGNSTGDNSTITIPCRIRQVINLWQEVGRAIYAPPIAGNITCKSNITGILLTRDGGQGANITNDTETFRPAGGDMRDNWRSELYKYKVVEIRPLGIAPTPAKRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAIERYLKDQQLLGIWGCSGKHICTTTVPWNVSWSNKTYDEIWKNMTWMQWDREIDSYTGTIYRLLEDSQIQQEKNEEDLLALDSWNNLWNWFSITKWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTLTHHQREPDRPERIEE"
  },
  {
    "reference": "MNLHELKPNESAVKDKKRIGRGLGSGKGKTAGRGYKGQKSRSGDNRVAPYFEGGQTPLHMRVPKVGFRSPFKKEYSVINLSMLEKHFNDGDEITKETLLAKSLIKKGKPVKILGMGEVTKKFKVKVDAISSSAKQKIEAAGGSVEILEAAGE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Binds to the 23S rRNA.. The designed protein sequence is ",
    "response#1": "ME",
    "response#2": "MPDTALRHXPIGANQSAFKSKRXASESLASELIAASNRDAKCFSINRKDGKERVAKAAL",
    "response#3": "GSRRSEAPKRQILPDPKYKSDLLAKFINTVMKDGKKSVAERVLYGALTQIEGKKGDGIQVLEQALDNVRPRVEVKSRRVGGATYQVPVEVRPVRQNALAMRWIVDAARKRGEKSMALKLAGELMDAADSKGSAVKKREDTHRMAEANR"
  },
  {
    "reference": "MSVQKDQMDLFALYKEEFYQSKNQLYMDGNSLGLMSKRSERKLIELADSWKVYGIEGWTEGNHPWFTLAENLSARIAPLAGALPHEVMVTGSITANIHQMLSTVFQPTEERFAIVVDELNFPSDIYAVESHLRLRGLDPATAMRKVKSRDGYTLQMEDIEEQLTEDVAVLLLPSVLYRSGQLLPMQDIAKAAHEKGILAGFDLAHSIGALPHDLHSIGADFAVWCHYKYMNSGPGGTGGLYIHERHHHLLPGNAGWFGSDKAKQFDMDHEFSKAEGAGAYQIGTPNIFSMAPLLGSVEIFEELGIDNIRKRSLQLTSRLRELIAEKLPEFQNVTPAGDKERGGHIALAHPEAARICKALKEEGVVPDFRAPDIIRLAPVALYISFEDVEEMVARLEKIMKQELYKNYSNERNVVA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The presence of pyridoxal 5'-phosphate is necessary for the protein to function in the desired environment.2. The pyridoxal 5'-phosphate binding site should be stable and able to withstand changes in environmental conditions.3. The protein contains novel Aminotransferase class V domains that confer a unique function or activity.4. Ideal protein should have a binding affinity for key enzymes in the quinolinate from L-kynurenine: step 2/3, Cofactor biosynthesis pathway to enhance the overall efficiency of the pathway.5. The protein design must be able to perform pyridoxal phosphate binding in order to contribute to anthranilate metabolic process, quinolinate biosynthetic process. The designed protein sequence is ",
    "response#1": "PRTARAAAEQQFRNWEQLAVEGHFKGETPWMHFHKRLEEASARVVGAKPVEVVVMNNLTTNLHLLLISFYQPTATRYKVLMEGGAFPSDQYALETQVRMRGLDPDDAIVELVPRPGEHTLRTEDILAKIAELGDSLCTILMGGINYYTGQVYDMAAITEAGHAVGAMVGFDLAHAAGNIPLHLHDWDVDFACWCSYKYLNSGPGGVSGAFVHERFH",
    "response#2": "PRTARAAAEQQFRNWEQLAVEGHFKGETPWMHFHKRLEEASARVVGAKPVEVVVMNNLTTNLHLLLISFYQPTATRYKVLMEGGAFPSDQYALETQVRMRGLDPDDAIVELVPRPGEHTLRTEDILAKIAELGDSLCTILMGGINYYTGQVYDMAAITEAGHAVGAMVGFDLAHAAGNIPLHLHDWDVDFACWCSYKYLNSGPGGVSGAFVHERFH",
    "response#3": "PRTARAAAEQQFRNWEQLAVEGHFKGETPWMHFHKRLEEASARVVGAKPVEVVVMNNLTTNLHLLLISFYQPTATRYKVLMEGGAFPSDQYALETQVRMRGLDPDDAIVELVPRPGEHTLRTEDILAKIAELGDSLCTILMGGINYYTGQVYDMAAITEAGHAVGAMVGFDLAHAAGNIPLHLHDWDVDFACWCSYKYLNSGPGGVSGAFVHERFH"
  },
  {
    "reference": "MIGAGPAGLAAAKTLAARGVSYRQFEKGSMVGGLWRIDNDNGAAAAYETLHLNSSRPRTAFPSHPMPEDWPDYPSHELVASYFQDLADRFGLTERITFNTEVVAVEPLDGDGPPGAEGWQVTTATGETVTTRAVLVANGHHGVPKLPELPGSFTGNWFHSHDYREPTVFDGQRVLVMGVGNSGMDIACDAAPLAEQVLLSTRHGVHVIPKYAFGKPVDQLSSPLTALLPFTVERALYEVLVRASVGRPQDRGLPRPDHRLLGAHPTVSSQLYDLVGHGDIEVVGDIEALEGDKVRFTDGRVEAVDVLVYATGYQVSLPFLAPAVLEVRDNELPLYQRVVAPERPGLYLLGFIQTVGANIALMEHQAEWVGDLLTGEAVLPSVAEMQDWVRRDRKAMARRYLRSERHTMQVDYWRYIRAIEQERQRKGQAGLRITPRLGDAVAVTRGVSGRASAALRLVSERAADRVGR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The desired protein should be able to perform H(+) + hexan-3-one + NADPH + O2 = ethyl butanoate + H2O +   NADP(+)2. A protein that contributes to lipid metabolic process by functioning in membrane with hypotaurine dehydrogenase activity, NAD(P)H oxidase H2O2-forming activity, N,N-dimethylaniline monooxygenase activity, NADP binding, flavin adenine dinucleotide binding. The designed protein sequence is ",
    "response#1": "GTI",
    "response#2": "LCF",
    "response#3": "MSRMTK"
  },
  {
    "reference": "MNIRPLHDRVIVKRLEVESTSAGGIVLTGSAAEKSTRGEVLAVGNGRILENGTVRPLDVKVGDVVIFNEGYGVKKEKIDGQEVLILSEADLMAIVG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein with metal ion binding is needed to enhance chaperone cofactor-dependent protein refolding in a specific context. The designed protein sequence is ",
    "response#1": "VHELRVSLEEIYHGSTKRMKITRRRLNPDGRTVRTEDKILHIVIKRGWKEGTKITFPKEGDATPDNIPADIVFVLKDKPHAHFRRDGTNVLYSALISLKEALCGCTVNIPTIDGRVIPLPCNDVIKPGTVKRLRGEGLPFPKVPTQRGDLIVEFKVRFR",
    "response#2": "ILLTNDDGISAKGMSLLVANLLKADFADLYIVAPATEQSGKSMSFSYTQPVSIEKVDYPQPVAGAWAVSGSPVDCVKLALGDLFRNALPDLVLSGINHGSNAGRNIFYSGTAGAAMEAILSGVPSIAFSQEQHISFFQEVHAPEILKKLSLYALSMPFPILSGFNVNFPASERNEEWKGMRLVTTGKR",
    "response#3": "IEHDLFVSLEEVDKGCIKKMKISRMATGSNGPYKEEKVLRITVKPGWKAGTKITFPQEGDSAPNKTPADIVFIIRDKPHSLFKREGIDLKYTAQISLKQALCGALVSVPTLQGSRIQVNPNHEIIKPTTTRRINGLGLPVPKEPSRRGDLIVSFDIKFK"
  },
  {
    "reference": "MFNNYGNVMMTDQFRKVEQYSPKSSPRAGGAGGRSPVVARQDSSGTLKTTIQLGKNPSIVHSGPFYLMKEPPGEGELTGATNLMAHYGLEHSYSKFSGKKVKEQLSSFLPNLPGVIDGPGHLDNSSLRSVIEKPPIGGKDLLPLTSVQLAGFRLHPGPLPEQYKHLKSVPTRKHKNKHKKHKYKEGVAPLSEQSALEASGLDTHEKKHKKQKRHEDDKERKKRKKEKKRKKQRHSPEHPGSGAASMPPQTQVF",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. The designed protein sequence is ",
    "response#1": "ERKGTKKEYMTRILDFQWCGNSKPILTPLGAMKTPQTEPPFYKNHIQNCTPIGVFHPASVKHAFTAIRTNGMVDFWYQLSNSKDFKKISLPVNQGSRQNEFEWLENASIAHMDEDQCFLIGTFSNVSQKINFYKLKVDWNVNGSTVNDPSLQLTHIYEMLPETTYKASVLLKLTNMKILSRTPIPNSKPEILLTYRSLNQNQSATTFRSIVKRYQITSTSLNETFLESLSIQVPSQRITHYCLKSMGTLELQNLLKEITAEDLCISTAFKYDDGHIEIYSRNNWKLQIGTESSTTGGYDKPFIQSMFSVGFKYQDC",
    "response#2": "ERKGTKKEYMTRILDFQWCGNSKPILTPLGAMKTPQTEPPFYKNHIQNCTPIGVFHPASVKHAFTAIRTNGMVDFWYQLSNSKDFKKISLPVNQGSRQNEFEWLENASIAHMDEDQCFLIGTFSNVSQKINFYKLKVDWNVNGSTVNDPSLQLTHIYEMLPETTYKASVLLKLTNMKILSRTPIPNSKPEILLTYRSLNQNQSATTFRSIVKRYQITSTSLNETFLESLSIQVPSQRITHYCLKSMGTLELQNLLKEITAEDLCISTAFKYDDGHIEIYSRNNWKLQIGTESSTTGGYDKPFIQSMFSVGFKYQDC",
    "response#3": "ERKGTKKEYMTRILDFQWCGNSKPILTPLGAMKTPQTEPPFYKNHIQNCTPIGVFHPASVKHAFTAIRTNGMVDFWYQLSNSKDFKKISLPVNQGSRQNEFEWLENASIAHMDEDQCFLIGTFSNVSQKINFYKLKVDWNVNGSTVNDPSLQLTHIYEMLPETTYKASVLLKLTNMKILSRTPIPNSKPEILLTYRSLNQNQSATTFRSIVKRYQITSTSLNETFLESLSIQVPSQRITHYCLKSMGTLELQNLLKEITAEDLCISTAFKYDDGHIEIYSRNNWKLQIGTESSTTGGYDKPFIQSMFSVGFKYQDC"
  },
  {
    "reference": "MSMERQLKEQFAENREIVEALLGDGSDADSEYVIEHHFSSTNFDKLEKAAVDAFKLGFEVNDAEEMELEDGSEIFCFDAIANHKLDVALLDKACEQLMQVAHKQKVDYDGWGTYFIGDEIEEDDEEEQA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must have high specificity towards endoribonuclease inhibitor activity, enzyme binding.2. The protein must exhibit the following characteristics: Globally modulates RNA abundance by binding to RNase E (Rne) and regulating its endonucleolytic activity; Can modulate Rne action in a substrate-dependent manner by altering the composition of the degradosome.. The designed protein sequence is ",
    "response#1": "AETREIIESLKADGSEPDALYAIEHHLMAEDFKALENAVVEAFKMGFEVLEAEELEDEDGAKILCCDAVMDSALDAEVIDAQVEKLVNLAEKYDIIYDGWGR",
    "response#2": "AETREIIESLKADGSEPDALYAIEHHLMAEDFKALENAVVEAFKMGFEVLEAEELEDEDGAKILCCDAVMDSALDAEVIDAQVEKLVNLAEKYDIIYDGWGR",
    "response#3": "AETREIIESLKADGSEPDALYAIEHHLMAEDFKALENAVVEAFKMGFEVLEAEELEDEDGAKILCCDAVMDSALDAEVIDAQVEKLVNLAEKYDIIYDGWGR"
  },
  {
    "reference": "MYQTLAISAAQGPGPYDGSAAGGFLHSAASASSPVYVPTTRVPGSMLPSLPYLQSGAAQPGSSGGGGSSAHAALWPQPGSAESPSSYSSSNGGGGRFAYSPSPPMANGGSREPGAYSSSLNARDQYRPLNGSYPGHYPSYVGPQLTPAWPTGHFENSMLHCLQSRPLPVRGGPSTDLLEDLSESRECVNCGSIQTPLWRRDGTGNYLCNACGLYTKMNGLSRPLIKPQKRVPSSRRLGLSCANCHTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMKKEGIQTRKRKPKNINKSKTCSSNNAVPMTPTSTSSTNSDDCSKNASPSTQTTASGVSSSVMSGQGESASPESSTLKYSGQEGLYSGVSLTSTAEVTASVRQDSWCALALA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be optimized for stability and solubility when expressed in nuclear membrane where it will be involved in cellular response to BMP stimulus.2. The designed protein must have at least one GATA-type domain. The designed protein sequence is ",
    "response#1": "GTLTYIQSRCQEALSNLHTDPETGSHRLVALLPATLQDCEEIHNEVEFEWLRQYWFQGQRYARFCSWWTRPMEQLEKDWKLMETMTMLFLAAVEEEGQAAEGRREMAEILLSALTDRHQQRQTWRDRCHSSLAQTLPPEEAPVDRPFWGVDDPSMPLPFDLADIINRVR",
    "response#2": "GTLTYIQSRCQEALSNLHTDPETGSHRLVALLPATLQDCEEIHNEVEFEWLRQYWFQGQRYARFCSWWTRPMEQLEKDWKLMETMTMLFLAAVEEEGQAAEGRREMAEILLSALTDRHQQRQTWRDRCHSSLAQTLPPEEAPVDRPFWGVDDPSMPLPFDLADIINRVI",
    "response#3": "GTLTYIQSRCQEALSNLHTDPETGSHRLVALLPATLQDCEEIHNEVEFEWLRQYWFQGQRYARFCSWWTRPMEQLEKDWKLMETMTMLFLAAVEEEGQAAEGRREMAEILLSALTDRHQQRQTWRDRCHSSLAQTLPPEEAPVDRPFWGVDDPSMPLPFDLADIINRVR"
  },
  {
    "reference": "MSINQDIQQHTLNTIEEAIQDIKAGKVLIVVDDEDRENEGDFVVAAELITPEIVNFMATHGRGLICTPLPEERCKELDLELMVGNNTALHATPFTVSVDLLGNGCTTGISASDRAKTIKSLVDPATKPEDLGRPGHIFPLKARQKGVIRRAGHTEAVVDLTRIAGLKPGGALVEIMNEDGTMARLPQLLKIAKKFDLKIISIADLIKYRLNEESLIERGARVKLPTEYGEFDLIPYIQKSNGLEHVALIKGEWTKDDTVCVRVHSSCVTGDIFGSKRCDCGQQLHEAMRKIEKAGKGVLLYMNQEGRGIGLFNKIKAYKLQENGRDTVEANVELGFEEDERDYGVGASILRDLGLGKIKLLTNNPVKRKGLEAYGLIVTENIPLIIEPNKHNKFYLETKRVKMGHTLELVPNHVHPKKGDTK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should consider the endogenous proteins and enzymes present in the 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4, Cofactor biosynthesis pathway to avoid interference with native metabolic processes.2. The Proton acceptor; for GTP cyclohydrolase activity of the protein must be engineered to enable effective catalysis of GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.3. The designed protein should have a high specificity for GTP binding, with minimal off-target effects.4. The designed protein should have zinc ion binding to facilitate riboflavin biosynthetic process. The designed protein sequence is ",
    "response#1": "RASEALVPTRLGGEFRAVVYENDIDHVDHVALVKGEIRPQDDVLVRVHSECLTGDTFGSLRCDCGEQLAAALKMIEEEGRGVLLYMRQEGRGIGLKNKIKAYALQDQEGLDTVQANERLGFPADMRDYGVGSQILVDLGVRRMRLITNNPGKRAGIEGYGLQIVERVGFGGSK",
    "response#2": "RASEALVPTRLGGEFRAVVYENDIDHVDHVALVKGEIRPQDDVLVRVHSECLTGDTFGSLRCDCGEQLAAALKMIEEEGRGVLLYMRQEGRGIGLKNKIKAYALQDQEGLDTVQANERLGFPADMRDYGVGSQILVDLGVRRMRLITNNPGKRAGIEGYGLQIVERVGFLAGEGQTEIVRVDMPTKFGDFTLVAFKEKLSSNEHLALIKGQWEKDEPVLVRVHSSCFTGDILGSLRCDCGDQLHKAMEMVQEEGKGAILYMNQEGRGIGLLNKLKAYRLQEEGMDTVEANLHLGFQMDQRDYGVGAQILRHLDITRLRLMSNNPRKRVGLIGYGLEIVENIDGRAGFFVRREAEVALPTAHGAFTAYGYRSATDGVEHVALVHGDLGDGEDVLVRVHSECLTGDIFHSLRCDCGPQLRESMQRVTEEGRGVVVYLRGHEGRGIGLLSKLRAYELQERGRDTLDANLELGLPADSRDYAAGARILDDLGVRSLRLMTNNPDKTAALMRHGLKVLDRF",
    "response#3": "RVSVNLPTLYGKFVLHLYRSFVNEYLHLALCLGEIGSLDNSPAPIQTEPVLIRVHDECLTGDIFGSSRCDCGEQLHNALKMIQQEGKGILLYMRQEGRGIGLENKLHAYALQENGFDTVEANKKLGFVADKRDYGIGAQILRDLGVSKMRLLSNNPKKFTALAGYGLEIVERIEADIFLPTAAGDFRMVGYSEEITGKEHVALVKGDLSSVDAPLVRVHSECLTGDVFHSERCDCGPQLDLAIAEIEARGVGAIIYMRQEGRGIGLINKLKAYELQEQGYDTVEANHQLGFDADLRDYAMSARILKDLGAETVTLLTNNPRKVEGLETYGVTVKAL"
  },
  {
    "reference": "MISEYVDEEIKRREDYSLRRLKGIPNDRRLWILTCMDERVHVEEALGIRPEDAHIYRNAGGIVTDDAIRSASLTTNFFGTKEIIVITHTDCGMIRFTGDEVAKYFLDKGVKVNELQIDPLLPSLRLQSTEDFTKWFKFFRDLGANSPDDIALKNAEILKNHPLIPKNVTISAYVYEVETHKLRKPHQRLYELTSRFEHGTVVKE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.2. A protein should be able to manipulate the activity of enzymes in the Sulfur metabolism pathway, either by activating or inhibiting their function.3. The designed protein should have zinc ion binding, hydrolase activity to facilitate hydrogen sulfide biosynthetic process.4. The protein should be designed to have high stability and activity towards carbon disulfide + 2 H2O = CO2 + 2 H(+) + 2 hydrogen sulfide.5. For general function, the protein need meet that Catalyzes the conversion of carbon disulfide into hydrogen sulfide and carbon dioxide, with carbonyl sulfide as an intermediate; Likely plays a key role in sulfur metabolism in S.solfataricus6. The protein should be able to bind Zn(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MMLRCRLPGGIMTPRQWLAIDKFATDNTIYGSIRLTNRQTFQFHGILKGNVKPAHQMLHENAEDEQEPFYGKTYMPRKFKIGFAVPPSNDIDVYSQDLGFIAVAEDGELKGFNVAIGGGMGRTDRAPETYPRLADVIGYVDADQVIPVVDAVMSVQRDFGDRTNRAHARFKYTVDDKGVDWIKAEIEHRLGL",
    "response#2": "MMLRCRLPGGIMTPRQWLAIDKFATDNTIYGSIRLTNRQTFQFHGILKGNVKPAHQMLHERWAKKLSEHLLPRTHAYAEIWLDKEKVATTDEEPILGETYLPRKFKTSVVIPPYNDVDLHANDMNFIAIAENGHLVGFNVLVGGGLAMTHGDKKTFPRLASEFGYIPIDKTLAVAEAIVTTQRDWGNRTERKNAKTKYTLERVGIETFKQEVERRSGLNSMACVAFPTCALAMAEAERYLPEFEQRLQQIIEQQGFKQEAIVVRMTGCPNGCGRAMLAEVGLVGKGPGKYNVHLGGNRNGTRIPRMYKENINEDEILAALEPLLARYAAERDPGEGFGDFVIRAGVIAPVIDSARDFYD",
    "response#3": "MMLRCRLPGGIMTPRQWLAIDKFATDNTIYGSIRLTNRQTFQFHGILKGNVKPAHQMLHEASFLGQEDEPILGNRYLPRKYKTAVVLPPLNDVDLYSNDMNFVGIKDEKTGKLAGFNVLVGGGLSFEHGNTKTYPNIALELGYVPVEDTLKAAESIVTTQRDFGNRADRKNARLRYTIQNMTLEGFREEVERRMGRSFLGQEDEPILGNRYLPRKYKTAVVLPPLNDVDLYSNDMNFVGIKDEKTGKLAGFNVLVGGGLSFEHGNTKTYPNIALELGYVPVEDTLKAAESIVTTQRDFGNRADRKNARLRYTIQNMTLEGFREEVERRMGRD"
  },
  {
    "reference": "MDWAEKYRPMHLADILGNGSAVRQIVDWAKTWTPDSRPLLFTGKPGIGKTSAALALARDMDWEVLELNASDARTKTIIERVAGNSSTTTSLFGAGRKLIIIDEVDNLEGNADRGGARAIADILKEAKQPIVLIANDAYGVSDSIRRLCDPVPFRAIGVSTLQKRMKEICRFEDIACGEDALSAIAESSAGDMRTAVNMLFGSSTGKTSISVGDINTAQKDERATIFDLVGGVFAGAPDRELQKLSFECDEKPDSVMQWIEESIPLMHDPKRRIRAYGRISRADVYLGRTMRRQYFTLWRYATSMMTLGVASENAGAGFRTRIMPPSRWKRMSTAKKQKTVRRTLAASLAEGYSIPERQIQSEYLDLLSRFAEKDPAAFCERHNLDVDQMGIILHDKAAAAAAVKTVQQAAKERDMKVKKMAASKKAEMRKLEEQLEALRMQEPPVPETPPAAEEQPLEEPQEEKKLAPKQATLDFF",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.. The designed protein sequence is ",
    "response#1": "SMPNLMFTGPAGVGKTTAALALAREILGEYWRQNFLELNASDARGIDTVRTSIKNFCRLKPVGAPFRIIFLDEVDNMTKDAQHALRREMEMYTKTSSFILSCNYSSKIIDPIQSRCAIFRFLPLKGN",
    "response#2": "GSKPLLLAGPPGVGKTSLALALANTMGWEAVELNASDQRSWRVIERIVGEGAFNETISDEGEFLSSRIGKLKLIILDEVDNIHKKEDVGGEAALIRLIKRKPAQPLILIANDPYKLSPELRNLCEMINFKRLW",
    "response#3": "EMTHFLFYGPPGTGKTSAILAMGREIFKEHFQNRVIEFNASDDRGINAVREKITNEAKKYVAEIKLEDGTIIPSYKIIILDEADSMTDEAQDALRVIIEQYSTATRFCFICNYITKITDAIKSRCSSVYFKKLI"
  },
  {
    "reference": "MGTPNDQAVLQAIFNPDTPFGDIVGLDLGEEAEKEEREEDEVFPQAQLEQSKALELQGVMAAEAGDLSTALERFGQAICLLPERASAYNNRAQARRLQGDVAGALEDLERAVELSGGRGRAARQSFVQRGLLARLQGRDDDARRDFERAARLGSPFARRQLVLLNPYAALCNRMLADMMGQLRRPRDSR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be able to modulate negative regulation of protein binding, central nervous system neuron development, tyrosine metabolic process, visual learning in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "SCSESKQKSECEECGGLGSLTGRCQWRQGHGEGITTNYSTCTPSLLTCPDGVCDAVESNNSTICPQDCTKITIIGGHEKGALSGIKAGYGTCFCLLPDKCFG",
    "response#2": "SPGELELEVTLDRQLYYHGEKISINICVRNNSNKVVKKIKAMVQQGIDVVLFQNGQFRNTIAFAESSEGCPLNPGSSLQKIMYLVPNLAANCDRAGIAVEGDVKHKNTSLASTTLIASQEARDAFGIIVSYAVKVKLFLGALGGELCAELPFILMHPKA",
    "response#3": "LYFLTKDYDENLYVDQPAGTLLLHIHVMKDSPHETPHFRLCPNSYLSRISFYHWFWIDEHTGILYLNRSLDRSDYEVFAQGSSLAVQKIILKVSTKPQPFLDNNCDNAPFTRVHLTFRNIR"
  },
  {
    "reference": "MGGKWSKSSIVGWPAIRERIRRARPAAEAAAEGVGTASQDLEKHGALTTSNTAQNNTACAWLQAQEEEDEVGFPVRPQVPLRPMTYKAALDLSFFLKEKGGLEGLVWSRKRQEILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPREVEEANEGENNCLLHPMSQHGMEDEEREVLKWKFDSGLARRHLAREKHPEFYKDC",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should include a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif to ensure proper function.2. For general function, the protein need meet that Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike3. The designed protein localizes to the extracellular region, membrane, host cell plasma membrane. The designed protein sequence is ",
    "response#1": "MPIGVPKVPFRSPGEPTNWLDLYNE",
    "response#2": "MELVDPRLEPWKHPGSQPKTACNKCYCKKCCFHCQVCFITKGLGISYGRKKRRQRRRTPQSTQDHQNPISEQPSXNPRGNPTGPEEPKKKVERETETDPDP",
    "response#3": "SXCCPIXGX"
  },
  {
    "reference": "MPKTIAELLVQTLADAGVERIWGVTGDSLNAVNWQLAKDGRIAFMHVRHEEAGAFAAGADAAVTGQLGVCAGSCGPGNLHLINGLFDCHRNHVPVLAIASHIPSSEIGLGYFQETHPTELFRECSHFCEMVTNPRQMPELLHRAMRTAIGKKGVAVIVLPGDVSLLEVPGNVPAFAPPSAAAPAPVPGDIEKAAAILSSAGRVAILAGSGCAGAHDAVVALADRLAAPVVHAFRGKEHVEWDNPFDVGMTGLIGFSSGYHALKECDALLMLGTDFPYRDFYPEHAKIIQVDVDPAALGRRTPLDLAVVADAGVFATALIPTIEEGRDRSFLDNAVRHYASARKGLDELAKPRPAGEPLHPQYVAAVLDKVAADDAIFTADVGTPVIWAARYLHMNGRRRLLGSFNHGSMANAMPQSLGAQAAFPGRQVISLSGDGGLAMLMGELLTTIQLKLPVKIVVINNGTLGFVEMEMKASGHPGTNVELQNPNFAEVAEAIGLKGIRVEDSEALEPALIEALAHDGPALVDVVTARHELSVPPRIQAAQAKGFSLYMLRAILSGRGDEVVELATVNLLH",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: A peripheral cell membrane enzyme that catalyzes the oxidative decarboxylation of pyruvate to form acetate and CO(2); It channels electrons from the cytoplasm to the respiratory chain at the cell membrane via ubiquinone..2. The Mg(2+), thiamine diphosphate binding site should be located in a region of the protein that is accessible to the ligand.3. The protein contains novel Thiamine pyrophosphate enzyme N-terminal TPP-binding domains that confer a unique function or activity.4. The designed protein should be involved in pyruvate catabolic process by localizing to plasma membrane and exhibiting pyruvate dehydrogenase (quinone) activity, flavin adenine dinucleotide binding, magnesium ion binding. The designed protein sequence is ",
    "response#1": "VQQAAAILNDAGAVTILAGAGVEGSHDAVITAATTLNAPIVHALRGKEFIEHDNPHDVGMTGLLGFPSGYKAIKEADALLMLGTDFPYQQFYPDNAKIIQVDIRGRNLGRRTPIELGLVGSVRDTLAAASVLLSRLSV",
    "response#2": "MNVAEHFVRELERAGVRRVWGLPGDSLNAFTDALRHSTQIDWMHVRHEEAAAFAAAAEAAVTGELAVVAGSCGPGNLHFINGLFDAQRSRVPVLAIASHIPSEQIGSGYFQETHPQELFRECSAYFDLVPVHPQYLARLISETAADDAVFTADVGTPVVWAARYLKMNGRRRLLGSFNHGSMANALLQAMGAQAASLQRQVISLSGDGGFTMMMGELLSLAQLRLPVKVVVLNNGTLGFVAMEMKAAGYLDYATDLQNPDFAAMAQAIGIKSWRVQDSAALPETLREAFAHPGPVLVDVHTAKQELTRAGDAWTVAENIVSVLRHHGVQRVYGLPGDSLNGFTDAIRRDGDIRWMHVRHEEAAAFAASAEAELTGELTVCAGSCGPGNLHLINGLYDANRSRVPVLAIAAHIPTAEIGSTYFQETHPQELFRECSVYVEYVADPVQMPRLLEIAMREAIEKRGVAVLVIPGDAEVLAPVRLN",
    "response#3": "PIRPEYVAGLVNRLAAEDAVFTVDVGSPVVWAARYLSMNGRRRLIGSFNHGTMACALPLAIGAQTAFRDRQVVALAGDGGLTMLFGELITLTQNRLPVKVIVFNNSSLNFVELEMKAAGIVNFGTDLHNPDFADVATAMGIFGRRVEHPAQLEQAVADALAHDGPAVVDVVTARQELGGRHG"
  },
  {
    "reference": "MFLSHQYDSFWIFLLVCISIPLLAFSITRFAAPPREGPEKSTSYESGIEPKGDTWIRFQIRYYMFALVFTVFDVETVFLYPWATSFEELGLFAFVEVIVFIFILIVGLVYAWRKGALEWC",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone; NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain The designed protein sequence is ",
    "response#1": "GHSSEPHESPLTMTFPLMALAVPSVLIGLLGRPWANQFEAFIHAPGEVVEHAAEFEWGEFYVMAGNSIGIALIGITVASLMYWQHKFDPKVLAEKFPSLYQLSLNKWYFDDLYDKLFVQGSRRVARQIMEVL",
    "response#2": "GHSSEPHESPLTMTFPLMALAVPSVLIGLLGRPWANQFEAFIHAPGEVVEHAAEFEWGEFYVMAGNSIGIALIGITVASLMYWQHKFDPKVLAEKFPSLYQLSLNKWYFDDLYDKLFVQGSRRVARQIMEVL",
    "response#3": "SPNLVQVYIFWELVGMCSYLLIGFWYDRKAAADACQKAFVTNRVGDFGLLLGILGLYWATGSFDFGTIGERLEGLVSSGVLSGAIAAILAILVFLGPVAKSAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARMYPVFEPIPVVMNTIAFTGCFTAFLGATIALTQNDIKKGLAYSTISQLGYMVMAMGIGAYSAGLFHLMTHAYFKAMLFLCSGSVIHGMEGVVGHDPILAQDMRIMGGLRKYMPITATCFLIGTLAICGIPPFAGFWSKDEILGLAFQANPLLWFP"
  },
  {
    "reference": "EMVALSGSHTIGQAR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must be engineered to have a cooperative binding mechanism with Ca(2+) to ensure proper regulation.2. The protein must exhibit the following characteristics: Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress; These functions might be dependent on each isozyme/isoform in each plant tissue..3. The heme, Ca(2+) binding site should be stable and able to withstand changes in environmental conditions.4. The designed protein should have metal ion binding, heme binding, lactoperoxidase activity within extracellular region to regulate hydrogen peroxide catabolic process, response to oxidative stress.5. We need a protein that can perform 2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2   H2O with high efficiency. The designed protein sequence is ",
    "response#1": "GLVMNFYKDSCPQAEDIIKEQVKLLYKRHKNTAFSWLRNIFHDCAVQSCDASLLLDSTRRSLSEKETDRSFGLRNFRYIETIKEAVERECPGVVSCADILVLSAREGIVSLGGPYIPLKTGRRDGRRSRADVVEEYLPDHNETISGVLDRFAAMGIDTPGVVALLGAHSVGRTHCVKLVHRLYPEVDPALSPDHVPHMLHKCPDQIPDPKAVQYVRNDRGTPMVLDNNYYRNILDNKGLLIVDHQLAYDKRTRPYVKKMAKSQDYFFKEFSRAITLLSENNPLTGSKGEIRKQCLQ",
    "response#2": "KVGFYSSTCPSAEAIVKSTVEKAISANPGIAAGLIRMHFHDCFVRGCDGSVLLASTPGNPISERDNFVNNPSLRGFEVIEDAKNQIEAACPETVSCADILAFAARDSVSKVGGISYDVPSGRRDGRVSIGDEVLDNLPRPSLSADDLISNFERKGLSADEMVTLSGAHSIGVSHCGAFSNRLYSFSDTVTQDPSLDSSYAETLKTQCPPPPPTSDPTVSLEPSTPIRLDSKYYEGLINHRGLLTSDQTLYTSQSTRGMVQSNANNGASWADKFALAMLRMGSIEVLTGSDGEIRKQCSFR",
    "response#3": "QLREGFYEHSCPQAEKIIKDYVMEHIPHVPSIAATLLRTHFHDCFVRGCDASVLLNATGGNEAERDAAPNQTLRGNGFIDRVKALVEKECPGVVSCADILALTARDSVVATGGPSWSVPTGRRDGTVSIKQEALDQIPAPTMNFTELLQSFQNKSLDLADLVWLSGAHTIGIAHCNSFTERLYNFTGRGGPGDADPSLDPLYAANLRRTKCKTPTDNTTIVEMDPGSFRTFDLSYYRGVLKRRGLFQSDAALITDAAAKADILTAVNSPPEFFSQVFARSMVKMGAIEVKTGSEGEIRKHCAIN"
  },
  {
    "reference": "MADQDNPVFQIQRVYLKDLSLEQPNSPAILLEQEQPNLDIQLGVEATPVGEGFFEVAVTATVQTKIKDKTMFLVEAKQAAIFEIRNVPEDQMGQIMGVVCPQIVYPYLRGNVADVINRAGFPPVHLGEINFQGMYEQQQAQAAGAAAPVTTQ",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that contributes to protein transport, protein folding, protein tetramerization by functioning in cytoplasm with unfolded protein binding.2. The protein must exhibit the following characteristics: It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state; It also specifically binds to its receptor SecA.; One of the proteins required for the normal export of preproteins out of the cell cytoplasm. The designed protein sequence is ",
    "response#1": "GHPK",
    "response#2": "LTASR",
    "response#3": "MG"
  },
  {
    "reference": "MSLSSKEIPIIDLDKENISNDVKAVPATGHKSLANQESFSQSQERRSHSVPAKTSNFKHSKPLTKHLSFAASSRLTTSQASINLSSSNSKGRKRKQSASHVSSLSKRRPLPWLPTPSLTYNPRLKGQSSQGESSSVNIGGLSNEQQLVLDAIMYSHKNVFFTGSAGTGKSFLLRTIIQRLRSRYGTSAVAVTASTGLAATNIDGTTINRFAGIGLGLESAVHLVGRVRKSRISTDRWKTTKFLIIDEISMIDAILFSKLNEVAKMVRGIEKPFGGIQLIITGDFFQLPPVSRNGTADFCFNCDTWKECIDETYLLTKIFRQSGDNQLIDMLNSLRLGRLSPYVKQQFKALERPLDYEDGILPTELYPTRDEVERANRNRLNSLAGEELTFFAKDSFGPEYAQLQGPPPPFLINLLNGLMCAKILKLKVGAQVMNLKNDMEDSAIVNGTIGKIVCFTTQEMWGFFCSKVKDYEMLIYQPLLAYASRFIGNPSADIDDKLYEEFVAANPSCLGKSTILPDLIRRAKRQKKEQLLPVVKFTVSSVDGYRLKLVEPVDFASESRSDVIRTQLPIILSWALSIHKSQGQTLNRVKVDLTRVFEKGQVYVALSRCVSTEGLEVVNFDESKVAVRKDVVRFYAKLKKQTNRASSRAKRG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase; Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction; DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability.2. The designed protein localizes to the nucleus.3. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "GTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQPPQFCFQAKSWRRCVPVILELTEVWRQADKTFISLLQAVRLGSLHS",
    "response#2": "LSEEQEHVIELARRKMNIFYTGSAGTGKSVLLRELIKTLKSTYGTSGAVAVTASTGLAACNIGGITLHSFAGIGLGVGDEAMLLKKVRRSAKHRERWKNIKALVIDEISMIDGTLLDKLDGIAKTLRRSSKPFGGIQIILCGDFFQLPPVTKGDQQIKFAFQSYSWRKAIDATIILKKVFRQQGDHLFINMLNEMRTGIISDR",
    "response#3": "LSHEQEYVLKQVLQGVSLFFTGPAGTGKSVLLRSIIKSLRSKRSRGIAVTASTGLAACNIGGITLHSFAGIGLGQGSVDSLYKKVRRNKTAFKRWQETKVLVIDEISMVDGNLLDKMNELAKKIRKSNAPFGGIQLVACGDFYQLPPVVKSHESESEEIHFAFECGAWSEAITQTITLKEVFRQKGDSTFINMLNEMRDGMINKR"
  },
  {
    "reference": "MSEPLLPGAAPEGGIAAVVVLAAGQGTRMRSSTPKVLHALGGRSMLGHVLAATAPLAPRHTLVVVGAGREAVEEHLAQVAPDARPVVQEDQRGSGHAAAVALAAVPDLQGAVLLVNGDAPLLRPETVTSLVDAHRSAGNVLTVLTAEVDDPTGLGRIVRDADGAVRAIVEQRDATPEQRAIREVNAGVYVGEAAALRRALGSLGEDNDQGEQYLTDVLGLLVAEGAPVGGAPAEDPVDVLGCNDRRELAARRRTLNDRVLDDLMRAGVTVVDPATTWVDVTVTVAADALLEPGVHLRGTTSVATGAVVGPDTTLVDTTVEEGASVVRSHVLDAVVGPRATVGPFSYLRPGTRLAAGSKVGAFVETKNVQVGEDSKVPHLSYVGDATIGRGSNVGAATVFVNYDGVEKHHTRVGDHVRIGSDTMLVAPVTVGDGAYTAAGSVITTDVPPGAMGVARARQRNVAGWVRRRRPGTAAAQAAEAAGDAGAETAADTRAEASE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The Proton acceptor of the protein must be engineered to enable effective catalysis of acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.2. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.3. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.4. The UDP-N-acetyl-alpha-D-glucosamine, Mg(2+), acetyl-CoA binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "VVVLAAGEGTRMKSATTPKVLHALAGRGMVGHVVEAARRLDPEELVVVVGHGRDRVVPYLAELDPGAFAVVQEQQNGTGHAVRVAVDALADQDREPSGTVVVVYGDTPLLTTETLGALIDTHQLEGNAVTVLSATVEDPTGYGRIVRSDAGDVVGIVEHKDASEAQLAITEINSGIYAFDAEVLRDALGKLSTDNDQGEEYLTDVLGILRAAGHRVGASVAADHREILGINNRVQLAEARRLLNDRLLSEAMLAGVTVIDPATTWVDVDVTLEADAVLLPNTQLHGTTHIAAGAEVGPNCTLRDTRVGAGAHVTNATCDGAVIGPDAAVGPYTYLRPGTVLAANAKAGGFVEMKNAQVGEGSKVPHLSYVGDATIGAGANIGAATIFVNYDGVAKHHTVVGDHVRVGSDSMLVAPVTIGDGAYTAAGSVITEDVPPGAMAVARGRQRNIAGWVERKRPGSAAAEAAERALAEQEPAAAEQATEE",
    "response#2": "IVLAAGQGTRMNSDLPKVLHQIAGAPMLGHAIHAGMALEPARIVVVAGHGAEAVTKAAEDINPFAQVVLQEEQLGTGHAVDQARDILGGFDGDVVVLYGDTPFIQPETLQAMAEARAKNDLIAIVGEADDPTGYGRVVRDASGAVQAIVEHKDADDAQRQVREINTGIMAAPTARLKQWLGALKNDNAQGEYYLTDIIAMAVADGVAVHTAQPAHAWETLGVNSKVQLAELERIHQRNVATLFMQQGVAIEDPETTFVGLDAEVEADALLRPFTLLEGRTRVAAGAMVGPFARLVDTTVGRGAQVLDHCLLRECVVEAEASVGPFAHVRPESRIGVKAKVGNFVELKKTRLGEGSKAPHLSYIGDATVGPGVNIGAGTITCNYDGVHKHPTRIEAGAFIGSDTTLVAPVTIGEGAYIAAGSAITEDVPAGALALGRARQRNIAGWKRPVKIQK",
    "response#3": "VIILAAGKGTRMRSNLPKVLQPLAGRPLLGHVIETAKKLNAENIITIFGHAGNLVQQAFSHENIQWVEQKEQLGTGHAVKVTLPVLPTEGVSLILSGDVPCINPTTLEKLLSVSRETGIGLVTLTLDNPTGYGRIIRDDDGNVLRIVEQKDASTEEKAIQEINTGIYCFNASLLKKYIPLLSNDNAQQEYYLTDVIEMARTELAQAEALFQARARAEALENGVTLQAPETVFFAHDTYVGRDTVVEPNVVFGPGVTVESGAEIRAFSHLEGCHVSQGAQVGPYARLRPGAELAEKVRIGNFVEVKNAILDEGAKVNHLSYIGDATVGAATNIGAGTITCNYDGVMKHRTEIGNHAFIGSNTMLVAPVKVGNRAMTGSGSVITKDVEADALAVSRARGNVEINRAGWRAELVRAEGIFQAKKRAEALENGVTMTAPETVFFGFDTYIGRDAVIEPNVVFGPGVTVESGATIRAFSHLEGCHVSRGAVVGPYARLRPGTELSENTKVGNFVEVKNATILEGAKVNHLSYIGDASIGARTNVGAGTITCNYDGFMKHRTEIGEGAFIGSNTMLVAPVKIGNEAMTATGSVINKDVPDGALALARARQRNIEGWVLKKRKGTATAKAAEAVKKENK"
  },
  {
    "reference": "MSDTRRSTGSARVLLIVGGGIAAYKALDVARRLQDHDIAVTGVMTGSASAFITPLSLAALTGKKTYTDLFSLTDEAEMGHIQLARQTDLVLVVPATANLMARTANGLADDLATTILLATTAPVMMAPAMNQAMWGHPATQANHTTLVARGIGMIGPDDGGMACGEEGTGRLSPTDEIVDAVVAKLAGRHKTLAGRHALVTSGPTIEPIDSVRFIANRSSGKQGHAIAAALAARGAKVTLVSGPVTLPQPPHVTHIAVESAREMMDACTAALPADIAICAAAVADWHVAGDTSGKLKKDNAGPPQIELVENPDILASLSAHKKRPQLVIGFAAEAENLRANAAAKRARKGCDWILANAVTRADGSSVFDSDSNGALLLAGNSCEDWPQMPKTALAERLADRIEAHFA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have FMN binding, phosphopantothenate--cysteine ligase activity, metal ion binding, phosphopantothenoylcysteine decarboxylase activity to facilitate pantothenate catabolic process, coenzyme A biosynthetic process.2. We require a protein that can specifically bind to the metabolites produced in the CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis, coenzyme A biosynthesis pathway.3. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.4. The protein contains novel Flavoprotein domains that confer a unique function or activity.5. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A6. The Proton donor of the protein must be engineered to enable effective catalysis of H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.7. The presence of Mg(2+) is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALKAAALLLSGGLALEGLNVVVTAGGTREPIDAVRVIANRSSGKQGHAISEECVRRGANVTVVTTTELPTSPTTDRIEVETAAEMQIALDNVAPTADIVVMAAAVADFRPKDSVDGKIKKAGGVPEVILEPTPDLLEAIGRNRHPHQTIVGFAAETCDLVTNARSKLERKCLDLVVANDVSAEGVGFAHGTNAVTLLAADGAVELAGRHVIVTAGPTQEPIDPVRVIANRSSGKQGFAIAGQLADLGARVTLVAGPVALDTPDGVDRIDVETAEQMQAAVAAALPADAAVLVAAVADWRVAAAAGKLKKADGPPALTWSENPDILAGLARDPRRPALLVGFAAETSDVVAAATAKRARKGCDWIVANDVSGDVMGGDRNAVHLITASGVEEWPRLPKDAVAEQLADRVVALL",
    "response#2": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALRIARGLLAPSGDLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHLLAADGREVALGPAPKEALADALWDAAVALLR",
    "response#3": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALAVLARPDLLAGRHVIVTAGPTQEPIDPVRVIANRSSGKQGFAIAGQLADLGARVTLVAGPVALDTPDGVDRIDVETAEQMQAAVAAALPADAAVLVAAVADWRVAAAAGKLKKADGPPALTWSENPDILAGLARDPRRPALLVGFAAETSDVVAAATAKRARKGCDWIVANDVSGDVMGGDRNAVHLVTAEGVESWDRLPRDEVATRLAAAVADALPEAGA"
  },
  {
    "reference": "MTDTVSFGYEEVAPEEKTRRVGDVFRSVAKRYDIMNDLMSAGMHRVWKDDFVRRVNPRAGEDILDMAGGTGDIAFRMEKSGANVTVADINPAMLEVGMERAAERELTSLIWREENAETLSFPSLSFDAYTIAFGIRNVTDIPAALMEAHRVLRYGGRFFCLEFSSTTWPGFAEVYDIYSEKLVPRIGEMVAKDGESYRYLIESIRRFPKMPEFAAMISAAGFSQVRYTPILGGAVAIHSGWKV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein with demethylmenaquinone methyltransferase activity, S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity, 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity, S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity, S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity functions is required to enable aerobic respiration, ubiquinone biosynthetic process, methylation, menaquinone biosynthetic process.2. The protein should be designed to catalyze the following reaction a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "MENKTFAFITAGGAGLRMGTSTPKQFLPLLGKPVLFHTLEKFAAHPQIDAIIVVISPKDKEYTQQILQEYHLEKVYQIVAGGKERQDSVYNGLKALAGDCDIYDVMNDLMSMGLHRARPFIPSSVIDQLLANIEKTGAAIAAVPVKDTIKVVDENYLQETVPRQQLWSAQTPQGFHYQIIMDAYQKAHEDNFVGTDEASLLEHQGHKVKITHGSYRNIKITRPEDLIIAEALLKAYDPNTEKTIMYDLMNDLMSGGLHRILYYAPDI",
    "response#2": "MMKKTYKILLAAHNQGKITEFAMLLKPFGFELISAKSFNLPEPIEDGTTFIDNALKKASIAAKHCHQPAIADDSGLCVEALNGMPGIYSARWAGPQNDAKTVNELLLKLQNVPDKNRTARFHCALVYMQHAKDPTPIICHGIWNGTIVNAPSGNNGFGYDPIFFLPKHQKTSAELLPELKNTISHRAKALKLFSEQLKID",
    "response#3": "MIEKLVIATHNKNKIKEIQYLLNGKNISIVSLDDYPQSPIVEEDGKTFQENASKKAITSCKMTNLPAFADDSGLVVDLLGGAPGIYSARYAGEDANDALNNEKLLKEMRHLKNRNAHFICALVYIDNQNYLFEGECIGLILEQKKGAGGFGYDPLFFYPPYKKTLAQLTKKEKNLISHRGKAIKKITDNFETWEK"
  },
  {
    "reference": "MMTPKPLSAELTTPRGAALRGRRGCRPAFGAWGEDLAAQYVESLGWTIIARNWTCDVGEIDLIARDDQTVVFIEVKARSGTGFGDPLESITTAKVRKLHELALAWLVNQDDGVHSVRIDAIGVMVRPGAEPTVTHVRGIR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be optimized for nucleic acid binding. The designed protein sequence is ",
    "response#1": "MPIGVPKVPFRSPGESDAAWF",
    "response#2": "SKIH",
    "response#3": "MR"
  },
  {
    "reference": "MSNSQSKKIFGLIGKHVDYSWSPLIHNTGFEALGLPCVYTIFNIPSPEMIGDALKGSRALGIAGFSVTIPYKKTVVPFLDELSPEALSIGAVNTIVNDNGRLLGYNTDIDGFAAPLLPMAESIRNRPVCIFGNGGAALAAVEAFRLRFNPSSVLLVVRDTQKAEDMLEEYAYRDLVTIHAGREIDQPACSKLIRDCRVLVNATPVGTAGRNDHIHSILPTGHGLLHDGQIVYDMVYNPPETPLLAEARAAGATVIAGIEMLIAQAARAFSIWTGQELPVDLVRKTVLAAIEKSEG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a fast turnover rate for NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH. The designed protein sequence is ",
    "response#1": "GMAEVIKTAAIWSEPDFVKLENGVESIRKAVLDSDSNTSDDTVQGAQEATRTDEQKLLLDVILASARVKAHVVTHDEREGGLRGLLNFGHSIGHGIEALVSPLLLHGECVSIGCVKEAELSRNLGHLNPVSVGRLARCLSSYGLPISLDEKKIKDRIGNLYCHVDDIMQVMKVDKKC",
    "response#2": "GMAEVIKTAAIWSEPDFVKLENGVESIRKAVLDSDSNTSDDTVQGAQEATRTDEQKLLLDVILASARVKAHVVTHDEREGGLRGLLNFGHSIGHGIEALVSPLLLHGECVSIGCVKEAELSRNLGHLNPVSVGRLARCLSSYGLPISLDEKKIKDRIGNLYCHVDDIMQVMKVDKKQ",
    "response#3": "GMAEVIKTAAIWSEPDFVKLENGVESIRKAVLDSDSNTSDDTVQGAQEATRTDEQKLLLDVILASARVKAHVVTHDEREGGLRGLLNFGHSIGHGIEALVSPLLLHGECVSIGCVKEAELSRNLGHLNPVSVGRLARCLSSYGLPISLDEKKIKDRIGNLYCHVDDIMQVMKVDKKR"
  },
  {
    "reference": "MNVTSNATAAGSFPLAFGLKTSFGFMHYAKAPAINLRPKESLLPEMSDGVLALVAPVVAYWALSGIFHVIDTFHLAEKYRIHPSEEVAKRNKASRMHVFLEVILQHIIQTIVGLIFMHFEPIYMTGFEENAMWKLRADLPRIIPDAAIYYGYMYGMSALKIFAGFLFVDTWQYFLHRLMHMNKTLYKWFHSVHHELYVPYAYGALFNNPVEGFLLDTLGTGIAMTLTHLTHREQIILFTFATMKTVDDHCGYALPLDPFQWLFPNNAVYHDIHHQQFGIKTNFAQPFFTFWDNLFQTNFKGFEEYQKKQRRVTIDKYKEFLQERELEKKEKLKNFKAMNAAENEVKKEK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that localizes to membrane and can perform sphingosine hydroxylase activity, iron ion binding with high specificity and efficiency.2. The designed protein should contain one or more Fatty acid hydroxylase domains that are essential for its function.3. The protein should contain a Helical membrane-spanning region.4. For general function, the protein need meet that Catalyzes the conversion of sphinganine to phytosphingosine in sphingolipid biosynthesis5. We need a protein that can perform an N-acyleicosasphinganine + 2 Fe(II)-[cytochrome b5] + 2 H(+)   + O2 = 2 Fe(III)-[cytochrome b5] + H2O + N-acyl-4-   hydroxyeicosasphinganine with high efficiency.6. We require a protein that can specifically bind to the metabolites produced in the sphingolipid biosynthesis pathway. The designed protein sequence is ",
    "response#1": "KTTADDSLPVLLEHRKEEIRAKQDFYHWRVFLPGLPSYVHIPSYRPPVRRHRNPNRPEWNGYIPGFPILINFKATKFLNLNLRYSFLKTASFFVRLGPMALAFKVRGLLDCKHSWKRLKDIRKIFPGKKSVVSEYVAEHWAEDTFFGYQYLNGVNPGLIRRCTRIPDKFPVTDDMVAPFLGEGTCLQAELEKGNIYLADYRIMEGIPTVELSGRKQHHCAPLCLLHFGPEGKMMPIAIQLSQTPGPDCPIFLPSDSEWDWLLAKTWVRYAEFYSHEAIAHLLETHLIAEAFCLALLRNLPMCHPLYKLLIPHTRYTVQINSIGRAVLLNEGGLSAKGMSLGVEGFAGVMVRALSELTYDSLYLPNDFVERGVQDLPGYYYRDDSLAVWNALEKYVTEIITYYYPSDAAVEGDPELQSWVQEIFKECLLGRESSGFPRCLRTVPELIRYVTIVIYTCSAKHAAVNTGQMEFTAWMPNFPASMRNPPIQTKGLTTLETFMDTLPDVKTTCITLLVLWTLSREPDDRRPLGHFPDIHFVEEAPRRSIEAFRQRLNQISHDIRQRNKCLPIPYYYLDPVLIENSISF",
    "response#2": "MIYVLGYSIYLSGYLKDYWCLPRPKSPPVDRITLSEYTTKEYGAPSSHSANATAVSLLFFWRICLSDTLVWPTKLLLLSLVIFYYLTLVFGRVYCGMHGMLDLFSGAAVGAICFFIRIWVVHAEHWAEDTFFGYQYLNGVNPGLIRRCTRIPDKFPVTDDMVAPFLGEGTCLQAELEKGNIYLADYRIMEGIPTVELSGRKQHHCAPLCLLHFGPEGKMMPIAIQLSQTPGPDCPIFLPSDSEWDWLLAKTWVRYAEFYSHEAIAHLLETHLIAEAFCLALLRNLPMCHPLYKLLIPHTRYTVQINSIGRAVLLNEGGLSAKGMSLGVEGFAGVMVRALSELTYDSLYLPNDFVERGVQDLPGYYYRDDSLAVWNALEKYVTEIITYYYPSDAAVEGDPELQSWVQEIFKECLLGRESSGFPRCLRTVPELIRYVTIVIYTCSAKHAAVNTGQMEFTAWMPNFPASMRNPPIQTKGLTTLETFMDTLPDVKTTCITLLVLWTLSREPDDRRPLGHFPDIHFVEEAPRRSIEAFRQRLNQISHDIRQRNPALFSAAEVQRRCAAGACWVRRGARLYDLTGFVRRHPGGEQLLRARAGRDVSADLDGPPHRHSDNARRWLEQYYVGELLV",
    "response#3": "WSAFSMGCAWQQLVFIAHDAGHISITHHYQLDNIFGMIIASWVGGLSLGWWKRNHNVHHLITNDPIHDPDIQHLPFFAVSTRLFDNIYSTYYEKFLWFDAFAKKVVPWQNYLYYPMLAFGRFNLYRLSWMHVLLGLGPRRGKAGWFRYFELCGLIFFNYWFFYLLVGCKLQTGWDRFQYIMVSHITTMLVHVQITLSHFAMSTSDLGVGEGFPMRQLRTSMDVDCPRWLDFLHGGLQFQVVHHLFPRLPRHNLRAAQPYVIEFCEKVGIKYSKTTADDSLPVLLEHRKEEIRAKQDFYHWRVFLPGLPSYVHIPSYRPPVRRHRNPNRPEWNGYIPGFPILINFKATKFLNLNLRYSFLKTASFFVRLGPMALAFKVRGLLDCKHSWKRLKDIRKIFPGKKSVVSEYVAEHWAEDTFFGYQYLNGVNPGLIRRCTRIPDKFPVTDDMVAPFLGEGTCLQAELEKGNIYLADYRIMEGIPTVELSGRKQHHCAPLCLLHFGPEGKMMPIAIQLSQTPGPDCPIFLPSDSEWDWLLAKTWVRYAEFYSHEAIAHLLETHLIAEAFCLALLRNLPMCHPLYKLLIPHTRYTVQINSIGRAVLLNEGGLSAKGMSLGVEGFAGVMVRALSELTYDSLYLPNDFVERGVQDLPGYYYRDDSLAVWNALEKYVTEIITYYYPSDAAVEGDPELQSWVQEIFKECLLGRESSGFPRCLRTVPELIRYVTIVIYTCSAKHAAVNTGQMEFTAWMPNFPASMRNPPIQTKGLTTLETFMDTLPDVKTTCITLLVLWTLSREPDDRRPLGHFPDIHFVEEAPRRSIEAFRQRLNQISHDIRQRNKCLPIPYYYLDPVLIENSIS"
  },
  {
    "reference": "MSAPAQPAPGVDGGDPSQARIRVPAGTTAATAVGEAGLPRRGTPDAIVVVRDADGNLRDLSWVPDVDTDITPVAANTDDGRSVIRHSTAHVLAQAVQELFPQAKLGIGPPITDGFYYDFDVPEPFTPEDLAALEKRMRQIVKEGQLFDRRVYESTEQARAELANEPYKLELVDDKSGDAEIMEVGGDELTAYDNLNPRTRERVWGDLCRGPHIPTTKHIPAFKLTRSSAAYWRGDQKNASLQRIYGTAWESQEALDRHLEFIEEAQRRDHRKLGVELDLFSFPDEIGSGLAVFHPKGGIVRRELEDYSRRKHTEAGYQFVNSPHITKAQLFHTSGHLDWYADGMFPPMHIDAEYNADGSLRKPGQDYYLKPMNCPMHCLIFRARGRSYRELPLRLFEFGTVYRYEKSGVVHGLTRVRGLTMDDAHIFCTRDQMRDELRSLLRFVLDLLADYGLTDFYLELSTKDPEKFVGAEEVWEEATTVLAEVGAESGLELVPDPGGAAFYGPKISVQVKDALGRTWQMSTIQLDFNFPERFGLEYTAADGTRHRPVMIHRALFGSIERFFGILTEHYAGAFPAWLAPVQVVGIPVADEHVAYLEEVATQLKSHGVRAEVDASDDRMAKKIVHHTNHKVPFMVLAGDRDVAAGAVSFRFGDRTQINGVARDDAVAAIVAWIADRENAVPTAELVKVAGRE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding.2. The presence of TGS domains is critical for the interaction of the designed protein with its intended targets.3. For general function, the protein need meet that Also edits incorrectly charged L-seryl-tRNA(Thr).; Catalyzes the attachment of threonine to tRNA(Thr) in a two- step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr)4. The presence of Zn(2+) is crucial for the protein to function optimally under specific conditions.5. The designed protein should be involved in threonyl-tRNA aminoacylation by localizing to cytosol and exhibiting threonine-tRNA ligase activity, tRNA binding, metal ion binding, ATP binding. The designed protein sequence is ",
    "response#1": "MSDVRVTVQRGQQAEERVVRTGTTAAELFDGERSVIAARVGGLQRDLSHPLAAGDVVEPIDYAIEADKKHLFLRFAACFGQFLMGHDMTFSYRSLPLRMFELTRYSFRREQRGELVGLRRLRAFTMPDMHTFCGDMSSAMEEFRKQYEASISVLRDAGLDLKDYEVAIRFTKDFYENNKTFIEGLVDIVDKPVLIEMWDERFFYFVLKFEFNFVDALDKASALSTVQIDVENAERYDIGYVDAEGNRQRPIILHCSPSGAIERLMYALLEG",
    "response#2": "DHRKLGKTMDLFHIQEEGKGMVFWHPKGWTLYLALEAYMRRRLDAAGYREVKTPQILDKSLWERSGHAEKFGHAMFMCESAEGEVLAVKPMNCPGHIQIFNVGQKSYRELPLRMAEFGACHRYEPSGAMHGIMRVRAFTQDDAHIFCREEQVTEESARFIELLRSVYSDLGMHLADTKFSTRPELRAGEDAVWDKAEAALSAAAEAAGETLVLQEGEGAFYGPKLEFSLKDAIGRVWQCGTLQLDFVLPERLDAEYVAEDGSKKRPVMLHRAILGSFERFIGILLENYAGHLD",
    "response#3": "MQLLLIHSDFIEFEAKKPTKFAEEVPEEARSGRLDEALCAFIAVEKFDEDDPDAVVAQAAGEIEEVAKRVKAERIMLYPYAHLSSALSSPETAVRVLRDLESTLKSSFEVARAPFGWYKSFTIRCKGHPLSELSRSGEVLTAPDYAIEADKKHLFLRFAACFGQFLMGHDMTFSYRSLPLRMFELTRYSFRREQRGELVGLRRLRAFTMPDMHTFCGDMSSAMEEFRKQYEASISVLRDAGLDLKDYEVAIRFTKDFYENNKTFIEGLVDIVDKPVLIEMWDERFFYFVLKFEFNFVDALDKASALSTVQIDVENAERYDIGYVDAEGNRQRPIILHCSPSGAIERLMYALLEEGLLSYAIEADKKHLFLRFAACFGQFLMGHDMTFSYRSLPLRMFELTRYSFRREQRGELVGLRRLRAFTMPDMHTFCGDMSSAMEEFRKQYEASISVLRDAGLDLKDYEVAIRFTKDFYENNKTFIEGLVDIVDKPVLIEMWDERFFYFVLKFEFNFVDALDKASALSTVQIDVENAERYDIGYVDAEGNRQRPIILHCSPSGAIERLMYALLEEGLLSPEQVRLLPVGDEQRPYAERVAAQLRERGVRVSVDRSGDRLGKQIRNAETQKIPVMGIIGAKEVEHETVSIRTRAAGDVGTFALEHLIAKLRAA"
  },
  {
    "reference": "MPRFVDRVVIHARAGSGGNGCASVHREKFKPLGGPDGGNGGRGGSIVFVVDPQVHTLLDFHFHPHISAPSGKQGMGNNRDGAAGADLEVKVPDGTVVLDENGRLLADLVGAGTRFEAAAGGRGGLGNAALASRARKAPGFALLGEPGETRELTLELKTVADVGLIGFPSAGKSSLVSAISAAKPKIADYPFTTLVPNLGVVSAGEHTFTVADVPGLIPGASAGRGLGLDFLRHIERCAVLVHVIDCATADPGRDPISDIDALEAELAAYTPTLQGDVTLGDLTERPRAVVLNKIDVPEARELAEFVRDEIAERGWPVFLVSTVAREGLQPLIFGLWQMISEYQSAQPEIVPRRPVIRPVPVDDSGFRVEPDPRQPGAFVVSGARPERWVRQTNFDNDEAVGYLADRLARLGVEEELLRLGARPGCAVTIGDMTFDWEPQTPAGQQVVLSGRGTDARLERTERVGAAERKAARRQRRTGDDAERGTTERGENT",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should contain one or more Obg, OCT, OBG-type G domains that are essential for its function.2. Mg(2+) is required for the proper folding and stability of the protein.3. The designed protein should be involved in ribosome biogenesis by localizing to cytoplasm and exhibiting GTP binding, GTPase activity, magnesium ion binding.4. The protein must exhibit the following characteristics: An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate; Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control..5. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.6. The designed protein should have a binding site that is specific to the target Mg(2+), GTP ligand. The designed protein sequence is ",
    "response#1": "ADVGLIGFPSAGKSSLVSTISAAKPKIADYPFTTLVPNLGVVSAGDHTFTVADVPGLIPGASEGRGLGLEFLRHIERCAVLVHVVDCATMEPGRDPISDIEALEAELAAYRPTLQGDSTLGDLAERPRAVVLNKIDVPDARELADFVRDEVAERFGWPVFEVSTVAREGLRPFIFALWDMVSDVGIIGMPNAGKSKFLTRCSNADTKVGDYPFTTIKPHLGVAKVDNSEVVIVDIPGIITDAHLGIGLGHKFLKHVERCKILLHLIDVTHDNVVSAYNCMRNELELYNSDLVKKEEIIVLNKCDLLRKVEIFEKKNHLANYLNKEVLCLSIGEDLQPILRLLNEKADVGLFGYSNTGRSCFMRSISNVKPKISFYPFTTLFPYIGSLNFLNKDIKFVDIPSFIEGKKKNNLRNRFLKHLQNCRLLLHFINLDVKNVKKTINKEKINIKILKSFRNLFLKPIWLIINKTDININSNIFFKQAQFVSNSLGYEKKFFFISLNDFISIKILINKILLFADIGLVGLPSAGKSTLLSVLSNAKPKIGDYDFTTLSPQLGLVKSSDSSYTIADLPGLIKGASEGKGLGIQFLKHIERCRVIAHIIDFGSKDKNPINAYEQILEELRLYDETLLERTQLIIANKEDLEDFETNLNLFRKKFPKLEIFQISALTTKNVNGLKNKLGEIADVGLVGFPSVGKSTLLSVVSAAKPKIADYHFTTIVPNLGVVETEDGRSFVMADLPGLIEGAHQGVGLGHQFLRHIERTRVIVHVIDMAAIEGRDPYEDYVVINEELKQYNLRLTERPQIIVANKMDMPNAEEHLQQFKQKLNEDVPIFPISAVTRQGIRELLFAIADLL",
    "response#2": "ADVGLVGFPSVGKSTLLSVVSAAKPKIADYHFTTIVPNLGVVETEDGRSFVMADLPGLIEGAHQGVGLGHQFLRHIERTRVIVHVIDMAAIEGRDPYEDYVVINEELKQYNLRLTERPQIIVANKMDMPNAEEHLQQFKQKLNEDVPIFPISAVTRQGIRELLFAIADLEAADALLADIGLVGLPSAGKSTLLSVLSNAKPKIGDYDFTTLSPQLGLVKSSDSSYTIADLPGLIKGASEGKGLGIQFLKHIERCRVIAHIIDFGSKDKNPINAYEQILEELRLYDETLLERTQLIIANKEDLEDFETNLNLFRKKFPKLEIFQISALTTKNVNGLKNKLGEILADVALLGLPNAGKSTLIRSVSEATPKVADYPFTTMYPHLGVVKVGVDSFVMADIPGVIEGAAEGAGLGLRFLKHLTRARCVLHVVDICPFNESDPVENYFAVEKELEKYSQELFDKPRFLVINKIDLLADKVEQKCQEFVEQIGYQGNYYTISAAMKKGTDELAKKLNEFLADVGLFGYSNTGRSCFMRSISNVKPKISFYPFTTLFPYIGSLNFLNKDIKFVDIPSFIEGKKKNNLRNRFLKHLQNCRLLLHFINLDVKNVKKTINKEKINIKILKSFRNLFLKPIWLIINKTDININSNIFFKQAQFVSNSLGYEKKFFFISLNDFISIKILINKILLFSH",
    "response#3": "MRFVDEVVIKLQAGKGGNGCVSFRREKYVPRGGPDGGDGGNGGSIYLKADENVNTLIDYRYKREYYAENGRPGEGRNCYGKAGEDLYLVVPVGTSVFDIDTNKKIGEVLQHGQTFKLVSGGKRGIGNTHFKSSTNQAPRKFTLGEEGEYKEVRLELNLIDIPGLIEGAHEGVPMVATKKVEKILREGADIGLVGLPSAGKSTLLSVLSNAKPKIGDYDFTTLSPQLGLVKSSDSSYTIADLPGLIKGASEGKGLGIQFLKHIERCRVIAHIIDFGSKDKNPINAYEQILEELRLYDETLLERTQLIIANKEDLEDFETNLNLFRKKFPKLEIFQISALTTKNVNGLKNKLGEILADVGLFGYSNTGRSCFMRSISNVKPKISFYPFTTLFPYIGSLNFLNKDIKFVDIPSFIEGKKKNNLRNRFLKHLQNCRLLLHFINLDVKNVKKTINKEKINIKILKSFRNLFLKPIWLIINKTDININSNIFFKQAQFVSNSLGYEKKFFFISLNDFISIKILINKILLFLG"
  },
  {
    "reference": "MRVTGIQRNCPQWWIWGILGFWMLMICKGKENLWVTVYYGVPVWKEATTTLFCASDAKAYNTEVHNIWATHACVPTEPNPQEIKLENVTEEFNMWKNDMIEQMHTDIISLWDQSLKPCVKLTPLCVTLNCHDVNVTNKSNKTDTEMAGEIKNCSYNMTTMLKDKKQQVYSLFYKPDVVEIEGNNNSGQYRLINCNTSAITQACPKVTFEPIPIHYCAPAGFAILKCNDEEFNGTGICKNVSTVQCTHGIKPVVSTQLLLNGSLAEKEIMIRSENITNNAKTIIVQLNESVTINCIRPNNNTRRSIRIGPGQTFYATGGIIGDIRKAHCNISKVAWNKALQKVAKKLRDRLNKTTIIFKPSSGGDPEITKHTFNCGGEFFYCNTSGLFNSTWNENDMNNTTTNSTDENITLTCRIRQIINMWQRTGQAIYAPPIEGLIKCSSNITGLLLTRDGGAKNNSQNETFRPGGGNMRDNWRSELYKYKVVKIEPLGLAPTRARRRVVQREKRAVGIGAVFIGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLKAIEAQQHLLKLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTNVPWNSSWSNKSLNDIWNNMTWMQWDREINNYTEIIYTLIEESQNQQEKNEQELLELDKWASLWNWFDISKWLWYIKIFIIIVGGLIGLRIVFAVLSVINRVRQGYSPLSFQIHTPNPGDLDRPGRIEEEGGEQGRDRSIRLVSGFFALIWEDLRNLYLFSYHRLRD",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein contains novel Retroviral envelope protein GP41-like domains that confer a unique function or activity.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in host cell plasma membrane that is critical for mitigation of host antiviral defense response. The designed protein sequence is ",
    "response#1": "MGAAATTLAVQTQKVMKGIVQQQDNLLRAIQAQQQLLRLSVWGIRQLRARLLALETLIQNQQLLNLWGCKGRLICYTSVKWNMSWANVTELDKIWGNLTWQEWDQQIDNISDTIYNEIQKAQIQQEVNEKKLLELDEWASIWNWLDITKWLWYIKIAIIIVGALIGVRIVMVVLNLVRNIRQGYQPLSLQIL",
    "response#2": "MGAASVTLTVQARQLLSGIVQQQNNLLKAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTTVPWNATWSNKSYHEIWDNMTWMQWEKEIENYTGLIYNLLEESQNQQDKNELELVELDKWANLWTWFGLNKWMRYIKIFMMIIGGLIGLRIVFAVLSIVNKVRQGYSPLSFQV",
    "response#3": "VEMKRCEFNVTTVLKDKKEKKQALFYRSDLMKLNNKTGDRNDTGMYTLINCNSTTIRQSCPKVSFQPIPIHYCAPAGYAIFKCNDTNFNGTGKCGNITVVTCTHGIKPTVSTHLILNGTLSEGKIRIMGKDISDTGKNIIVTLNSTVNITCVRPWNQTVQTIGIGPMSWYSMGITADNNNNSRIAYCKYNTTNWERALKQTAERYLELMNHTDGANISMIFNHSSDGDPETTHLHFNCHGEFFYCNTSKMFNYTFLCKGTNCTNTQSNSTRNDTVIYCKIRQVVRSWMRGGSGLFAPPIPGNLTCRSQITGMMLQMDKPWNGSNSNSTFRPTGGDMKDIWRTELFKYKVVRVKPFSVAPTKIAR"
  },
  {
    "reference": "MTGEKIRSLRRDHKPSKEDGDVLEPCEEEATAALGGAFTGGRSGPGGSGKGGKACHKIFSHHHRLQLKAPAVPGPGVPAPPQHNAVTATCPAPACLGGSNPALVADGGGCPSLYPVHECVFKGDVRRLSSLIRTHNIGQKDNHGNTPLHLAVMLGNKECAHLLLAHNAPVKVKNAQGWSPLAEAISYGDRQMITALLRKLKQQSRESVGEKRPRLLKALKELGDFYLELHWDFQSWVPLLSRILPSDACKIYKQGINIRLDTTLIDFTDMKCQRGDLSFIFNGDAAPSESFVVLDNEQKVYQRIHHEESEMETEEEVDILMSSDIYSATLSTKSISFTRAQTGWLFREDKTERVGNFLADFYLVNGLVLESRKRREHLSEEDILRNKAIMESLSKGGSLAEQSFEPVRRQSLTPPPQNTITWEEYISAENGKAPHLGRELVCKESKKTFKATVAMSQEFPLGIESLLNVLEVIAPFKHFNKLREFVQMKLPPGFPVKLDIPVFPTITATVTFQEFRCDEFDGSIFAIPEDYKEDPSRFPDL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be expressed specifically in the endoplasmic reticulum, perinuclear region of cytoplasm The designed protein sequence is ",
    "response#1": "LTNCCNTSDVTFCSEFTCLHSTGCVVCEQDGNRTLCWEPAGPMVSHRPNYSGVDPWLSAHIDFVAGSIYFCDLSAMHEVCGLLALASSYFVEHMPITITLNTTAPCYLLAETGANPSITGFFGWLASEVSMVTGLVDTLWKVPAAVGYAFGQKHYITLAAITGLALNGNIPKALALTLLN",
    "response#2": "LTNCCNTSDVTFCSEFTCLHSTGCVVCEQDGNRTLCWEPAGPMVSHRPNYSGVDPWLSAHIDFVAGSIYFCDLSAMHEVCGLLALASSYFVEHMPITITLNTTAPCYLLAETGANPSITGFFGWLASEVSMVTGLVDTLWKVPAAVGYAFGQKHYITLAAITGLALNGNIPKALALTLLF",
    "response#3": "LTNCCNTSDVTFCSEFTCLHSTGCVVCEQDGNRTLCWEPAGPMVSHRPNYSGVDPWLSAHIDFVAGSIYFCDLSAMHEVCGLLALASSYFVEHMPITITLNTTAPCYLLAETGANPSITGFFGWLASEVSMVTGLVDTLWKVPAAVGYAFGQKHYITLAAITGLALNGNIPKALALTLLF"
  },
  {
    "reference": "MMVCPGKPGASLLMLSMLFHIIDSYPNNEMARGGCDECRLQENKIFSKPSAPIFQCVGCCFSRAYPTPLRSKKTMLVPKNITSEATCCVAREVTRLDNMKLENHTDCHCSTCYYHKF",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in positive regulation of steroid biosynthetic process, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "NWNVQCWVTGDLKLLICAVEPSRQNPCKNYDLHLFYALLEVAGGSRLAPQKGSFEIVQCNCSSEDRWECRVPVPAAKLHDSFLMYLKITAAWATFWSPLMSVQPQ",
    "response#2": "IKGFGGSLSDAAAINILGLSRPAQDHLLRSYFSESGIEYNLIRLPMACSDFSVRPYSYDDVPNDYELKHFMLAEEDVNMKVGSGGHWSSAMSKRPLSLFASPWTSPAWMKSNGDVRGKGTLKGQAGDKYHKTWANYFIKFLDEYSKHNVTFWAVTAQNEPLAALLTPPLFPTIAFTAEHQRDFVIQDLGPALARSPHRTLLIILDDQRIHLPHWAKVVLGNTTAARYVAGMGVHWYLDSIVPPSWSLEATHKLFPDHFLLYTEACSGFLTFRFSVSLGCWERGDRYSHSILTVLNNFVAGWTDWNLALDMKGGPNWVKNFVDSPVIVDSSKDVFYKQPMFYHMGHFSKFL",
    "response#3": "NINISCETDGYLTKMTCRWSANPMQSLVGSSWKLRYYRRSLYCSERPSIHPVSEPKDCHLQDDGFYECIFQPIFLFSGYTMWIRINHTLGSLDSPPTCVTPS"
  },
  {
    "reference": "MLRYCLHRLLIGLGMLLALTILIFVLLQLTPGDPIDAYINPNVAMTQAEMDALRAQLGLDRPLPVQYLAWLGQAVQGNLGHSLQRFNETVSGLIASRIGPTLLLMAAGLAIAIVIGVTTGIISAVRRNSFPDYSFSVLALLGISSPAFLTALLGLYVFSVRLKWAPSGGMLTPATDFSIPDLLRHLALPALVLSIGHAALIMRYMRSSMLETLNQDYVRTARAKGVREFWVVVKHTLRNAMLPVVTLIGSTIGLAVGGAIFIESVFNWPGMGLLLINAVETRDYPVIMGATLVIGACVIIVNILTDLAYAVIDPRIKVT",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Probably responsible for the translocation of the substrate across the membrane .. The designed protein sequence is ",
    "response#1": "NVYLLGFTSFLNDMSSEMIMPILPMLITSVGGGSLSIGLVGGLREFISNILMVLIGYCSDKVRKRKIFVVLGYLTSSMFKLLLGLSKSWLGAVIFSSLERMGKGIRTAPRDAIISESMPKTLGKGFGIQRAFDTAGAILGSTLSLLFILYLQYSFNQIILIAAVIGFLTLIPLYFVKEKPSPSNNKITFRVGIKNLPKELKLFILISAIFTLSNFSYMFYILRAQEFLMIVDEKMAIIIPIALYILYNIFYATFSIPFGILSDKIGRKSVLTIGYIVYGIVSLGFAYFISQKSLILLFALYGIAYALFAGNQKAYVSDLSSEDIRATALGLFYTVVGLTSLPASLIAGYLWKISPEMTFLYGSVLAIISGLLLLFAR",
    "response#2": "NVYLLGFTSFLNDMSSEMIMPILPMLITSVGGGSLSIGLVGGLREFISNILMVLIGYCSDKVRKRKIFVVLGYLTSSMFKLLLGLSKSWLGAVIFSSLERMGKGIRTAPRDAIISESMPKTLGKGFGIQRAFDTAGAILGSTLSLLFILYLQYSFNQIILIAAVIGFLTLIPLYFVKEKPSPSNNKITFRVGIKNLPKELKLFILISAIFTLSNFSYMFYILRAQEFLMIVDEKMAIIIPIALYILYNIFYATFSIPFGILSDKIGRKSVLTIGYIVYGIVSLGFAYFISQKSLILLFALYGIAYALFAGNQKAYVSDLSSEDIRATALGLFYTVVGLTSLPASLIAGYLWKISPEMTFLYGSVLAIISGLLLLFS",
    "response#3": "NVYLLGFTSFLNDMSSEMIMPILPMLITSVGGGSLSIGLVGGLREFISNILMVLIGYCSDKVRKRKIFVVLGYLTSSMFKLLLGLSKSWLGAVIFSSLERMGKGIRTAPRDAIISESMPKTLGKGFGIQRAFDTAGAILGSTLSLLFILYLQYSFNQIILIAAVIGFLTLIPLYFVKEKPSPSNNKITFRVGIKNLPKELKLFILISAIFTLSNFSYMFYILRAQEFLMIVDEKMAIIIPIALYILYNIFYATFSIPFGILSDKIGRKSVLTIGYIVYGIVSLGFAYFISQKSLILLFALYGIAYALFAGNQKAYVSDLSSEDIRATALGLFYTVVGLTSLPASLIAGYLWKISPEMTFLYGSVLAIISGLLLLFAR"
  },
  {
    "reference": "MSPAMLQASSLCVSAALSGAASRPGRLASQGHQGKRAVAQPLAASAVTEAAPPAPVVAPPARPVDAPRRRGGRGGGGGGGELVAWKSVRQERWEGALEVDGELPLWLDGTYLRNGPGLWNLGDYGFRHLFDGYATLVRVSFRGGRAVGAHRQIESEAYKAARAHGKVCYREFSEVPKPDNFLSYVGQLATLFSGSSLTDNSNTGVVMLGDGRVLCLTETIKGSIQVDPDTLDTVGKFQYTDKLGGLIHSAHPIVTDTEFWTLIPDLIRPGYVVARMDAGSNERQFVGRVDCRGGPAPGWVHSFPVTEHYVVVPEMPLRYCAKNLLRAEPTPLYKFEWHLESGSYMHVMCKASGKIVASVEVPPFVTFHFINAYEETDEEGRVTAIIADCCEHNANTAILDKLRLHNLRSSSGQDVLPDARVGRFRIPLDGSQFGELETALDPEEHGRGMDMCSINPAHVGREYRYAYACGARRPCNFPNTLTKVDLVERTAKNWHEEGSVPSEPFFVPRPGATEEDDGVAISMVSAKDGSGYALVLDGKTFEEVARAKFPYGLPYGLHCCWVPRKRNSK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a fast turnover rate for all-trans-10'-apo-beta-carotenal + O2 = (2E,4E,6E)-4-   methylocta-2,4,6-trienedial + 13-apo-beta-carotenone.2. The protein must exhibit 9-cis-10'-apo-beta-carotenal cleavage oxygenase activity, carotenoid dioxygenase activity, all-trans-beta-apo-10'-carotenal cleavage oxygenase activity.3. The protein must exhibit the following characteristics: Produces the C(19) carlactone and a C(8) hydroxyaldehyde.4. The presence of Fe(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "FGWREGLLLPVIVTVYALILGDRWMYVVFWPLPSILASIQLFVFGTWLPHRPGHDAFPDRHNARSSRISDPVSLLTCFHFGGYHHEHHLHPTVPWWRLPSTR",
    "response#2": "FGWREGLLLPVIVTVYALILGDRWMYVVFWPLPSILASIQLFVFGTWLPHRPGHDAFPDRHNARSSRISDPVSLLTCFHFGGYHHEHHLHPTVPWWRLPSTG",
    "response#3": "FGWREGLLLPVIVTVYALILGDRWMYVVFWPLPSILASIQLFVFGTWLPHRPGHDAFPDRHNARSSRISDPVSLLTCFHFGGYHHEHHLHPTVPWWRLPSTNV"
  },
  {
    "reference": "MTNQYDATIYYDDDADDSYLNDKTVAVLGYGSQGHAHAQNLADSGVDVVVGLREGSSSRKAAEADGLRVATPKEAASEAQVVSVLVPDTVQPAVYADIESELEPGDTLQFAHGFNIHYGQIEPSEDVDVTMIAPKSPGHLVRRNYEADEGTPGLLAIYQDASGEAKEEALAYGKAIGCTRAGVVETSFQEETETDLFGEQAVLCGGVTELIKAGYETLVDAGYSPEMAYFECLNEMKLIVDLMYEGGLGEMWDSVSDTAEYGGLTRGEVVVDDHARENMETVLQQVQDGEFARQWIVENQAGRPSYRQKRKAEKNHEIEEVGDRLRQLFSWSETEDEESESEEGEQERVTADD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. We require a protein that can specifically bind to the metabolites produced in the L-valine from pyruvate: step 2/4, L-valine biosynthesis, Amino-acid biosynthesis pathway.2. A protein with magnesium ion binding, ketol-acid reductoisomerase activity functions is required to enable valine biosynthetic process, isoleucine biosynthetic process.3. For general function, the protein need meet that Involved in the biosynthesis of branched-chain amino acids (BCAA); Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate; In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.; In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB)4. The protein contains novel KARI C-terminal knotted, KARI N-terminal Rossmann domains that confer a unique function or activity.5. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "MFYEKDADVDLIKSKKIAIFGYGSQGHAHALNLKDSGAKEVVVALRDGSASKAKAESKGLRVMNMSDAAEWAEVAMILTPDELQASIYKNHIEQRIKQGTSLAFAHGLNIHYKLIDARKDLDVFMVAPKGPGHLVRSEFERGGGVPCLFAVHQDGTGKARDLALSYASAIGGGKSGIIEDSKLKGKTVAIIGYGIQGRGQALNLRDSGVKVIVAQRPGGPNFDLAKQDGFTPLSAAEATKKADVVILLAQDMLQGDIYREAIAPNLRPGQTLGFSHGFAVLYGLIKPPKNVDVVMVAPKGPGSLVRSQYLEGKGVPALVAVYQDASGKAKATALAWAKGIGATRAENMTMSVASFVAEVKSDLMGEQTILCGMLQAGSLLCYDKLVADGAEPGYAGKLIQFGWETITEALKQGGITLMMDRLSNPAKLRAYALSEQLKEMMTPLFQKHMDDIISGEFSSGMMADWANDDKKLLGWREETGNTSFTFKDETETDLFGEQAVLCGGTEELVKAGFDVMVEAGYPPEMAYFEVLHELKLIVDLMYEGGIARMNYSVSDTAEFGGYLSGPRVIDADTKERMRGILRDIQDGSFVKKLVANVEGGNKQLEVLRKENAEHPIEVTGKKLRDLMSWSNEHIDEQTYFDKGIFLVAMIKAGVELAFDTMVDAGIIEESAYYESLHETPLIANTIARKRLYEMNVVISDTAEYGCYLFNHAAVPLLRDYVKSMSAEYLGGGLKDSSNAVDNVRLIEVNQAIRNTAVEFIGEELRGYMKDLITL",
    "response#2": "MATIYYDKDADLKALKGKTVAIIGYGIQGRGQALNLRDSGVKVIVAQRPGGPNFDLAKQDGFTPLSAAEATKKADVVILLAQDMLQGDIYREAIAPNLRPGQTLGFSHGFAVLYGLIKPPKNVDVVMVAPKGPGSLVRSQYLEGKGVPALVAVYQDASGKAKATALAWAKGIGATRAGVLEHLRGKKVVIVGCGAQGLNQGLNMRDSGLDVAYALRAEAIAERRDSYRRATEHGFCVGDYQALIPQADLVINLTPDKQHAAVVSAVQPLMKPGAALGYSHGFNIVEVGQQIRPDITVVMVAPKCPGTEVREEYKRGFGVPTLIAVHPENDPRGAGMAIAKAWASATGGDRAGTIASGCSFAFKEETETDLFGEQAVLCGGTSALVKAGFETLVEAGYQPEIAYFECLHELKLIVDLMYEGGLEYMRYSISDTAQWGDFQAGPRIVTDETKAAMKEILSDIQKGKFAKGWILENKLNRPEYNAINEAEKNHPLEVVGRELREMMAQLGKCRFMARTEFADGVNALRGKKVVIVGCGAQGLNQGLNMRDSGLDVAYALRAEAIAERRDSYRRATEHGFCVGDYQALIPQADLVINLTPDKQHAAVVSAVQPLMKPGAALGYSHGFNIVEVGQQIRPDITVVMVAPKCPGTEVREEYKRGFGVPTLIAVHPENDPRGAGMAIAKAWASATGGDRAGVLEN",
    "response#3": "MATIYYDKDADLKALKGKTVAIIGYGIQGRGQALNLRDSGVKVIVAQRPGGPNFDLAKQDGFTPLSAAEATKKADVVILLAQDMLQGDIYREAIAPNLRPGQTLGFSHGFAVLYGLIKPPKNVDVVMVAPKGPGSLVRSQYLEGKGVPALVAVYQDASGKAKATALAWAKGIGATRAGVLEXAXTXKGIVANPNCSTIQMVAAVXAFLVAMIKAGVELAFDTMVDAGIIEESAYYESLHETPLIANTIARKRLYEMNVVISDTAEYGCYLFNHAAVPLLRDYVKSMSAEYLGGGLKDSSNAVDNVRLIEVNQAIRNTAVEFIGEELRGYKGAATNTVQIAELLVAQLGKCRFMAREEFVDETGYLKGKKVVIVGCGAQGLNQGLNMRDSGLDIAYALRKEAIEEKRASWRKATENGFQVGTYEELIPQADLVINLTPDKQHSSVVSAVQPLMKEGAALGYSHGLNIVEVGEQIRKDITVVMVAPKCPGTEVREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGAHRAGVLEXKGXIXN"
  },
  {
    "reference": "MALNERNGMSGRDDRSAGAAPGKPGVPTVAVVVVAAGRGTRAMRPDDNTPKQFVPIAGRAVIAKTLAGFSAHPRVARTLAVIHRDDGGVFADAVAGIEGLDTPEPGGATRQISVLAGLERLARDNPPDLVLIHDGVRPFCSAAVVDRVIDALGSADGALAALPVADTMKRVDDRGRIVDTVPRTALWAAQTPQGFRFPAILEAHRRAAAAGKTELTDDAAVAEWAGLEVVVVNGDSANVKITTADDLSKADAKLTLERWLALADVRVGTGFDVHVFGPGSFVTLGGIEIPHTHGLVGHSDADVVLHALTDALLGTIGDGDIGQHFPPSDPKWRGAASSLFLEDAVRRIRERGGEIAQLDATVVCEVPRIGPHRDAMRARIAEVAGIALDRVGIKATTSEGLGFTGRREGIVAMATATVRLPLGR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. We are looking for a protein with broad substrate specificity for 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-   erythritol 2,4-cyclic diphosphate + CMP.2. The designed protein should have a high specificity for a divalent metal cation binding, with minimal off-target effects.3. The protein must exhibit the following characteristics: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D- erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C- methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF)..4. The protein design should incorporate a binding pocket that specifically recognizes a divalent metal cation to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "AQVAQAAGLTIALVEGDETLQKLTFASDFAADTPPIRIGSGYDVHRLEDGEELWLCGVKIEHHQGLAGHSDADVAIHALVDAMLGAMGDGDIGQHFPPSDAQWRGASSDRFLAHAVGIVAKGGYRLGNADVTIICEAPKIGPYRDTMRDRLAAILGVDKSLVSVKATTTEKLGFTGRGEGIAAQAVVSLVVR",
    "response#2": "SRSATGYDVHRLEAGEELWLGGVLIPHDRGLSGHSDADVALHAITDALLGTIGAGDIGTHFPPSDPQWRGAESGQFLQHAASLVRAEGGIIDFVDLTLICEAPKIGPHRAAMRSRISDLLDIAERHVSIKATTSERLGFTGRQEGIAAQAIATIRDDASIAEWAGLTVELVEGSPDNVKLTVQRDIAMADAKLSAALPDVRTGNGYDVHQLEPGDGVTLCGIFIAHDQALKGHSDADVALHALTDALLATCGAGDIGDHFPPSDPQWRGAASRIFLEHAAKIVRDAGGTIMNADISLIAEAPKIGPHRQAMREKLSEILGISLDRCSVKATTNEKIGFVGRREGIAAIATATVLRKLREAQVAQAAGLTIALVEGDETLQKLTFASDFAADTPPIRIGSGYDVHRLEDGEELWLCGVKIEHHQGLAGHSDADVAIHALVDAMLGAMGDGDIGQHFPPSDAQWRGASSDRFLAHAVGIVAKGGYRLGNADVTIICEAPKIGPYRDTMRDRLAAILGVDKSLVSVKATTTEKLGFTGRGEGIAAQAVVSLVEG",
    "response#3": "IGQGYDVHRLVEGRKLIIGGVEIPFEKGLLGHSDADVLAHAVTDSLLGAAAMGDIGTLFPDTDPAYKGADSMALLKKVVGLISIRGFSIGNIDATVIAQSPKLSPFRDAIVKSMCKAMGIRENQFNFKAKTEEGLGFTGAGEGISAQALALLG"
  },
  {
    "reference": "MAKENFDRSKPHLNIGTIGHVDHGKTTLTAAITKVLSESGLSEVKDFDQIDNAPEEKERGITINTSHVEYQTKNRHYAHVDCPGHADYVKNMVTGAAQMDGAILVVAATDGPMPQTREHILLGKQVGIPRIVVFMNKVDLVDDAELLELVEMEVRDLLSFYDYDGDNCPVIAGSALGALNGEAEWSKKIVELMDSVDTWIENPVREVDKDFLMPVEDVFSITGRGTVATGRIEAGVASTGESVDIIGMGAEKLASTITGVEMFRKILDRGEAGDNVGLLLRGIEKTDIKRGMVICKPGSVTPHDKFKAEVYILKKEEGGRHTPFHNNYRPQFYVRTTDVTGTINLPEGVEMVMPGDNLTINVDLHHPIAMNVGLRFAIREGGRTVGAGETKGGKPVPMSETEINKMLGRFDRMSLEPENVVIPYEVGDVVKIVDGAFKGFHEISKVVKLQVKGGVANPSPPVGPALGAAGVNIMEFCKQFNARTQDKQGKVLPVVINVYKDKSFDFVVKTPPAAVQLLEAANIKKGSDVAVRLGVDAKKANQMVRGVVMLPHGTGKQTKVLALVTADKEGEAKQAGADYVGLDEYLDKIKGGWTDVDVIVTMPSVMGKLGPLGRILGPRGLMPNPKTGTVTMDVKKAVSDVKAGKIDFKIDKTGIIHAVVGKASFSVEQLVENTEELMQNIVNLKPKASKDLKDFAEQLVNLTVKEVNELATILKDEHGIEPAAAAVAIAGNDTGVAEPVEEKSEFDVVLKAAGGSKLAVVKLVKELT",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have translation elongation factor activity, ribonucleoside binding, acyl-[acyl-carrier-protein] desaturase activity, DNA-directed 5'-3' RNA polymerase activity within large ribosomal subunit, nuclear lumen to regulate fatty acid biosynthetic process.2. The protein must exhibit the following characteristics: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates..3. The designed protein have Tr-type G domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MFSGQGAQYVNMGWELYQTEPIFQEQVDKCSEYLKPFLGRDLRLVLYPELLYSPFSKGVIQTAERAISEQEATELLTHTAIAQPALFVIEYALAQLWMAWGVHPEAMIGHSIGEYVAACLSEVFSR",
    "response#2": "RWRRAASIVLAAGWSRPETATPPSRPPPAEGFRLLLLQRSPHQGFMPGAHVFSGGVLDAADRSADWLGLFAPHHGPPRFGLGPAPFSRTAFPSLPDTDDHKTDNTGTLPEDVAFRICAVREAFEEAGVLLLRPRTSPPGPAPGPGLALEPPPGLASWRDRVRQDPRHFLRLCAHLDCTPDIWALHNWSAWLTPFLRGTTRRFDTAFFLCCLREPPPVYPDLAEVVGYQWSSPSEATESFLSKEIWLPPPQFYLD",
    "response#3": "RWRRAASIVLAAGWSRPETATPPSRPPPAEGFRLLLLQRSPHQGFMPGAHVFSGGVLDAADRSADWLGLFAPHHGPPRFGLGPAPFSRTAFPSLPDTDDHKTDNTGTLPEDVAFRICAVREAFEEAGVLLLRPRTSPPGPAPGPGLALEPPPGLASWRDRVRQDPRHFLRLCAHLDCTPDIWALHNWSAWLTPFLRGTTRRFDTAFFLCCLREPPPVYPDLAEVVGYQWSSPSEATESFLSKEIWLPPPQFYH"
  },
  {
    "reference": "MFGLIHNPPHAGRLPAAATTLVEARGLTVRRGDQPVLCNVDLVVESGQVVTLVGPNGSGKSTLVQVLTGVIRDYRGQVGRRTGLRVGYVPQHFRIDRNLPITVRRFMKLAPRGRRGHWAEAVADAGVSQLLDRPMQGLSGGELRRVLLARALLSRPHLLALDEPAAGLDQRGQGELYRLIRHVRDRYGCGVLVVSHDLNLVMAATDQVLCLSEGHILCRGAPESVRAHPEYRALFGAHLGPETAVFPHAHGPGEGCTHG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Responsible for energy coupling to the transport system.; Part of the ABC transporter complex ZnuABC involved in zinc import.2. The ATP binding site should be stable and able to withstand changes in environmental conditions.3. The protein can be targeted to plasma membrane and perform ABC-type zinc transporter activity, ATP hydrolysis activity, ATP binding. The designed protein sequence is ",
    "response#1": "IEFRNVSKKFGNKTPISKVSFIVKKNNITTLIGPNGAGKTTIVRLMLGLEKPTSGEVIIDRKLKIGYVPQKFGLTTDIPITVKKFLDLLAPSHFNKNIKEISSFIDLEHIKKQEISKLSGGQFQKVVLACSIINNPDLIILDEPLQSLDVTSQQEFYQLIHFIRKKLNITVFMISHDLFTVIKNSDQVICLNGHICCSGVPHEITPNSEFSNALSSC",
    "response#2": "IEFRNVSKKFGNKTPISKVSFIVKKNNITTLIGPNGAGKTTIVRLMLGLEKPTSGEVIIDRKLKIGYVPQKFGLTTDIPITVKKFLDLLAPSHFNKNIKEISSFIDLEHIKKQEISKLSGGQFQKVVLACSIINNPDLIILDEPLQSLDVTSQQEFYQLIHFIRKKLNITVFMISHDLFTVIKNSDQVICLNGHICCSGVPHEITPNSEFSNALSSC",
    "response#3": "SKVSFIVKKNNITTLIGPNGAGKTTIVRLMLGLEKPTSGEVIIDRKLKIGYVPQKFGLTTDIPITVKKFLDLLAPSHFNKNIKEISSFIDLEHIKKQEISKLSGGQFQKVVLACSIINNPDLIILDEPA"
  },
  {
    "reference": "MQELCPGLFFGNVSTCCDILQLQTLKNNLQLPLQFLSRCPSCFYNLINLFCELTCSPNQSEFLNVTSTIPYYDPVSKENKSSITELQYFIGERFANEMYNACKDVEAPSSNVKALGLLCGKDAKDCNATNWIEYMFSKDNGQTPFSIIPIFSDVPVHGMNPMNNATKGCNESVDDSTGPCSCQDCSIVCGPKPQPPPLPVPWLLFGLDAVYIIMWISYMGFLLVFFALVFGVWCYRRRHFVSEYTPIDSNVAFSVNSHHDNGKITCGERLGERFENGLRMTFTSWGAFCVRNPRPVILFSVVFIAMCCSGFVYIKATTNPVDLWSAPSSQARKEKEYFDKHFGPFFRTEQLIIQAPNSRPSIYSPYPSGADVPFGPPLDKDILHQVLDLQNAIVNIVASFDNETVMLKDICVAPLAPYNNNCTILSVLNYFQNSHSVLDHAVGDEFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGAFGGPVFPWLVLGGYDGDNYNNATALVITLPVNNYYNDSKKIMKALAWEKEFVNFLKNYNNPNLTISFSAERSIEDEINRESRSDVSTVLISYIVMFVYISIALGHIQSWGRLLVDSKISLGIAGILIVLSSVACSIGIFSYFGVPVTLIVAEVIPFLVLAIGVDNIFIIVQTLQRDERLEGETLDKQIGRVLGDVAPSMFLSSFSETVAFFLGTLSTMPAVRTFSLFAGMAVLIDFILQVTCFVSLLGLDIRRQERNRLDILCCIKGSEEMRGVQRSESILFLFFKNLY",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in Golgi apparatus, membrane raft that is critical for endocytosis, membrane raft organization.2. The protein should have a fast turnover rate for cholesterol(in) = cholesterol(out).3. The protein should contain a Helical membrane-spanning region.4. The designed protein must have at least one SSD domain. The designed protein sequence is ",
    "response#1": "MGAASLTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLQDQRLLGIWGCSGKLICTTTVPWNDSWSNRSYDSIWDNMTWMQWEKEIDNYTGVIYNLLEKSQNQQEKNEQELLELDKWASLWNWFDITKWLWYIKIFIMIIGGLIGLRIVCAVLSIVNRVRQGYSPLLFQTN",
    "response#2": "MGAASLTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLQDQRLLGIWGCSGKLICTTTVPWNDSWSNRSYDSIWDNMTWMQWEKEIDNYTGVIYNLLEKSQNQQEKNEQELLELDKWASLWNWFDITKWLWYIKIFIMIIGGLIGLRIVCAVLSIVNRVRQGYSPLLFQTN",
    "response#3": "GCIWYGQSHMIGAHWVNKGDTNPARPLNSPTSEAIFAKRCPMLYKEYKGESGEDELSLCCDAAQIETMESGLSQADGVFSRCPTCTRNMALTVCAMTCAKNHTLFLTAYNDTNDAGVDYVKYIDYRLTDDTVSKIYNSCIGIQHTQTGRPAMDLGCGSYNAKTCNYRRWYEFMGDVSGDYVPFQINYKWSEDAEEGSNEIYLDLSPLKCGESYEDSYACACIDCEESCPLTDAPTGPDELWKIAGLA"
  },
  {
    "reference": "MQPAALLGLLGATVVAAVSSMPVDIRNHNEEVVTHCIIEVLSNALLKSSAPPITPECRQVLKKNGKELKNEEKSENENTRFEVRLLRDPADTSEAPGLSSREDSGEGDAQVPTVADTESGGHSRERAGEPPGSQVAKEAKTRYSKSEGQNREEEMVKYQKRERGEVGSEERLSEGPGKAQTAFLNQRNQTPAKKEELVSRYDTQSARGLEKSHSRERSSQESGEETKSQENWPQELQRHPEGQEAPGESEEDASPEVDKRHSRPRHHHGRSRPDRSSQEGNPPLEEESHVGTGNSDEEKARHPAHFRALEEGAEYGEEVRRHSAAQAPGDLQGARFGGRGRGEHQALRRPSEESLEQENKRHGLSPDLNMAQGYSEESEEERGPAPGPSYRARGGEAAAYSTLGQTDEKRFLGETHHRVQESQRDKARRRLPGELRNYLDYGEEKGEEAARGKWQPQGDPRDADENREEARLRGKQYAPHHITEKRLGELLNPFYDPSQWKSSRFERKDPMDDSFLEGEEENGLTLNEKNFFPEYNYDWWEKKPFEEDVNWGYEKRNPVPKLDLKRQYDRVAELDQLLHYRKKSAEFPDFYDSEEQMSPQHTAENEEEKAGQGVLTEEEEKELENLAAMDLELQKIAEKFSGTRRG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should emphasize Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "VIKSLNINSNISICDSLGRTLLHLACLKNYSSLVYTLIKKGARVNDIDSFGLTPLHFACISGDPE",
    "response#2": "LPSINRVIPSQGPINGGIEVTLLGCNFKDGLSVKFGSNLALSTQCWSETTIVTYLPPAAYAGQVFVSITDTNNENNNDDLPQEIEINDNKKAIFTYR",
    "response#3": "LPSINRVIPSQGPINGGIEVTLLGCNFKDGLSVKFGSNLALSTQCWSETTIVTYLPPAAYAGQVFVSITDTNNENNNDDLPQEIEINDNKKAIFTYR"
  },
  {
    "reference": "MTDTHAASAPPSSTSSVTPLRAETPVPLPDALAVVEGGGVTSARGFRAAGVHAGFRKDPSRFDLALVAADKPCVCAAVFTQNVFCAAPVTVSREHLEDCGYGIAQALVVNSGNANAATGERGLAAARETARIASDVLGCAPAEVLVASTGVIGEHLPLEPFATGIPLAALELSVVSGGANAARAIMTTDTRPKEAAVTFSGDGIGYDGCTFTVGGMAKGSGMIMPNMATMIAVLTTDAPIRPDALHAALSRAVGRSFNKVTVDSDTSTNDSCFLFSNGGATSSTVPFHPGSPALARFEQALEAVCQNLARQMAADGEGATRLVTVNVAGAATPQDADAAARAVANSPLVKTAVYGHDANWGRVAMAIGKSGAAFRQQDAAIDIMGMPVCRGGLTVPFDEDEALRRFENPEIVIDVDLGAGDAATTVWTCDFSHDYVTINGDYRS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate..2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.3. The designed protein should be involved in arginine biosynthetic process by localizing to cytoplasm and exhibiting methione N-acyltransferase activity, acetyl-CoA:L-glutamate N-acetyltransferase activity, glutamate N-acetyltransferase activity.4. The protein should be optimized for activity in N(2)-acetyl- L-ornithine from L-glutamate: step 1/4, L-arginine biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. The substrate binding site should be stable and able to withstand changes in environmental conditions.6. The designed protein is the optimization of the Nucleophile to achieve high catalytic efficiency for acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate. The designed protein sequence is ",
    "response#1": "MTVARRDARGPVAGGDARLAAALDALRDGAVVVLVDDEDRENEGDLVVAAERATPEIVAFMVRYTSGYVCVALAGPDCDRLDLPPMYTVNQDARGTAYTVSVDAAPAHGVTTGISAADRAATVRALADPDTRPADLVRPGHVFPLRAREGGVLVRPGHTEAAVDLARLAGLRPAGAICEVVSEDGSMARLPELRRFADEHGLTLISIADLIAYRRRHEKHVVRVGGEVPQVTAHEPELRTRTTERLGHLVAVPLPPVTVPANDENLRYLRTKRDRMGHLFTDPGAAGAADAAPADPAPVAGPAPAADPAPAGPASAAGPAPAGPAPAGPAPAAPAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGPASVGGPAPAGPAPAGPAPAGPAPAGPAPAGPAPAGPAPAGPTPAGPAPAGPAPAGPAPAGPASAGPAPAGPASAGPAPAGPAPAGPAPAGPAPAGPAPAGPAPAGRAPAGPARAGPASAGPASAGPASAGPASAGPASAGPASAGPASAGPASAG",
    "response#2": "MSKKYMNIADLGIENAPKKNTISKDLEIENINFDSRKITSNSIFVALVGENNDGRDFIKEVINKGANTVICELEGNKVEGYEDIKIISTNSIKNELAKIALKFYPKKPKIIAXIGANGKTTTKEIIKQILSISGKKAFLSKGNFNNEIGLPKTILNLNDKYKFSILESASSGLKKGRLKYENFEIGIFTNLSLEHLDYHKTIENYKKIKTKLFESLKKNSFAIINKDDENFYNIFKNSSKYKIITFSLKNKNSNFKAENIKISNKGTSFTLIKNKKKLKIKLPLVGKHNIYNSLCAIAIATNLGLSLKDIKKSLENIKGIKNRLEFIKIKKKNILINDTYNSSINSLEIALKSIKEIKGKKKILVLGDMLELGKNEEKLHLEVGKKISKVNIDKLITIGELSKFIYNSLEKKNFKKDKIKYFKNNFILAQYIKNISYKNSVVMIKSSNSSKINEIVESVL",
    "response#3": "MNDPKPAPSNAAPTSAAQADSVRLGAXAXLPAXRXNXQPXGXGXAXPXVXAXPXRXDXRXAXDXPXTXPXGAXDXXAXAAWFXPXPXTT"
  },
  {
    "reference": "MRAYEVVYVLTPDLEEEQVEEKHNEFKSVIERYGGAPGEIDVWGKRRLAYEIDDYQEGIYALRKFQGENQVLTELERSLKMDESILRYLIARDEDA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have rRNA binding, structural constituent of ribosome within ribosome, ribonucleoprotein complex to regulate translation.2. The protein must exhibit the following characteristics: Binds together with S18 to 16S ribosomal RNA.. The designed protein sequence is ",
    "response#1": "MNGKKFIPVFVTENMVGHKLGEFAPH",
    "response#2": "MSFLGL",
    "response#3": "LIVGH"
  },
  {
    "reference": "MLDRFLPEFDRALRAVAGITRASRPNPADAIVAPTDDGAKLSDTERRHAAGLMRVNHVGEVCAQALYQGQALFARDPAIRAQLDEAAREEEDHLAWCAQRLQELHDRPSLLNPLWYAGAFAIGALAGRLGDKISLGFVAETERQVEHHLDGHLDRLPEHDARSRAIVAQMRDDEVRHGDNARAAGGIDLPEPVRQAMRLASRIMTTAAYRI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have 3-demethoxyubiquinol 3-hydroxylase activity to facilitate ubiquinone biosynthetic process. The designed protein sequence is ",
    "response#1": "LDIGCGGGLLSEPMARLGAAMVSADASEQNIKTASVHAAEQGLGIDYRCTTAEALAAAGETFDVILNMEVIEHVADPMAFLKDCASMLRPGGLMFK",
    "response#2": "LDIGCGGGLLSEPMARLGAAMVSADASEQNIKTASVHAAEQGLGIDYRCTTAEALAAAGETFDVILNMEVIEHVADPMAFLKDCASMLRPGGLMFG",
    "response#3": "LDIGCGGGLLSEPMARLGAAMVSADASEQNIKTASVHAAEQGLGIDYRCTTAEALAAAGETFDVILNMEVIEHVADPMAFLKDCASMLRPGGLMFG"
  },
  {
    "reference": "MGKTIADSLVEALLNAGVKRVYGIVGDSLNALIDSIRRSGEIEWIHVRHEEVAAFAAGSDAEVSGSIAVCAGSSGPGNLHLINGLYDCQRNRVPVLAIAAHIPSSEIGSNYFQQTRPEHLFKDCSHFCEVIMTPGQMPRTVTMAIQNAVSNKGVSVVVLPGDVAGLPDSTPVPEHVYHVTTPVVHPSDEELQQLASYLNMGKRITLLCGSGCAGAHKQLMELCEKLQSPMVIALRGKEYLEFDNPYLVGLTGLIGYSSGYHAMMDCDVLLMLGTDFPYRQFYPEKAVVLQVDIQKDHLGRRTPLTYGLCGDVKNTIESLMPLLTESHDSKHLNKCVSDYAEVRKDLDDLAIGKPGRKPIHPQYLTKVVSDLANEDAIFTADVGTPTLWGARYLQMNGKRRFIGSFNHGTMANALPQAIGAKVATPNRQVIALAGDGGLSMLMGDLITLIQHKLPVKIVVFNNSALSFVELEMKAAGYLETGTELVGQNFANIAKAIGIEGYRVENPEDLEGVIKQAFTSDAPAIVDVVVNRQELSLPPKITFEQAQGFTIWMLKAVLNNRGSELIELAKTNLFR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: A peripheral cell membrane enzyme that catalyzes the oxidative decarboxylation of pyruvate to form acetate and CO(2).2. A protein with ubiquinone binding is needed to enhance pyruvate catabolic process in a specific context.3. The designed protein should be able to compete with endogenous proteins or inhibitors for FAD, Mg(2+), thiamine diphosphate binding. The designed protein sequence is ",
    "response#1": "IERLAGLLNGSTKVTILAGSGCAGQHDAVVALAAALKAPMVHALRGKEHVEWDNPYDVGMTGMIGFSSGYHAMMSCDTLLMLGTDFPYRAFYPREAQVIQIDRDPGALGRRVPLTQGLVGDVGETIAAVE",
    "response#2": "IEDAAAALNGSRKVTILAGAGTEGAHPELIAIAHALQAPIVHSMRGKEFVEYDNPFDVGMTGLLGFSSGYHALKSCDTLLMLGTDLPYQQFYPDEATVIQVDVRGSQIGKRTKVDIALTGTVADTVNAAS",
    "response#3": "DRLAALLDGAEAVTLLCGAGCAGAHDEVVALADLLAAPVVHALRGKEHVEWDNPFDVGMTGLIGFSSGYYAMESCDALLMLGTDFPYRQFYPTKAVVAQIDLRPEVLGRRTRLDMALVGDVGPTLRAQ"
  },
  {
    "reference": "MEFPFDINHLFPERFSILDQTLVAGRKTAGMPHLQANIETVIDELGRASAKAQQLPASITSASKLQSQKHQLYLLKDGESNGGRGVVVGFLKVGYKKLFLLDRQGSHVEAEPLCILDFYIAENLQRYGYGRELFDFMLQHKKLEPVLMAYDRPSPKFLSFLAKHYCLTPSVPQANNFVVFEGFFVNIAGSPSSPLPDQGMFSFFGPAEKGSPKKAGRRDQALLFNGERSGPSGAAGSPLAVCSASLPAAVGILPVLPLPERGVLPQQAPPPGRTSSCPWAQQGAEPPVPPPRALQGKTQQRAGWGGHLQPVPGAPEQQSSWTAAQTFPRSKPLSLPPLFASKKPLASSVYRGTQLGGAEEELRGLDSLQRAPRGMLFNQRRLLERESPGAGGGGGALQERAATARSG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for stability and expression in focal adhesion, axon where it will participate in neuron development, alpha-tubulin acetylation, regulation of microtubule cytoskeleton organization.2. The protein must exhibit the following characteristics: Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules; May be involved in neuron development.. The designed protein sequence is ",
    "response#1": "LYNSLNSLMIHLCPSRFSHFLMTSYTPLRQVNQISSRTSAIDVMKRLIQPQNIMARTRLKEGKYISMCDFFQGDVSYDEINEALQRFTDRRLAEFVPWNKEAIKVVHTRVSPLVKRGNRMSGMLLANNTSIRYYFQDILN",
    "response#2": "KGHETVCVVFTNRHKNLDTVRAAWSTGDIKTSMDSAVAINDLSVVVDLLNIVNQKASLWKLDLCTIVLPQIEKLLQSKYESYVQTGCTSLKLILQRFLPLITDILAAPPSVGVDITREERLHKCKLCYKQLKNISNIIKSKSGLSGRHGSAFCELHLLC",
    "response#3": "MEFPFDVDALLPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIVDELGKASAKAQHLPAPIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQHLPAPITSASRMQSNRHVMYILKDTSARPAGKGAIVGFLKVGYKKLFVLDDREAHNEVEPLCILDFYIHESLQRHGHGRELFQHMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFA"
  },
  {
    "reference": "MEVVEAAAAQLETLKFNGTDFGVGEGPAAPSPGSAPVPGTQPPLQSFEGSPDAGQTVEVKPAGEQPLQPVLNAVAAGTPAPQPQPPAESPACGDCVTSPGAAEPARAPDSLETSDSDSDSDSETDSDSSSSSSSSSSSSSSSSSSCISLPPVLSDGDDDLQIEKENKNFPLKTKDELLLNELPSVEELTIILPEDIELKPLGMVSSIIEQLVIIESMTNLPPVNEETVIFKSDRQAAGKIFEIFGPVAHPFYVLRFNSSDHIESKGIKIKETMYFAPSMKDFTQYIFTEKLKQDKGSDASWKNDQEPPPEALDFSDDEKEKEAKQRKKSQIQGRKKLKSEFNEPGEDFTEVHQNWNAHSSASEHAKGYRNREFTRGFSRARYPRSCHGRPPPQHFYNSEHMVSQETSGFPSQRQNNPIMPQYPFPLPVFDMHNFPLRPPPPPPPPPVNMGWATPNMAAHPLLNLPYSLPPPPPPPPLPPPPSSGDSNSHFGPYY",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have identical protein binding, RNA binding, telomerase RNA binding within sno(s)RNA-containing ribonucleoprotein complex, cytoplasm, nucleoplasm to regulate positive regulation of telomerase activity, RNA stabilization, positive regulation of telomerase RNA localization to Cajal body, positive regulation of telomere maintenance via telomere lengthening.2. Prioritize Polar residues, Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.3. The protein design should prioritize the formation of strand-strand-strand-strand-strand-strand-helix-strand elements.4. The protein must exhibit the following characteristics: Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.; RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. The designed protein sequence is ",
    "response#1": "LLDDLDKELNTYAMRCKEWYGWHFPELAKIVTDSVAYARIILTMGIRVNAAETDMSEILPEEIEERVKTAAEVSMGTEITPVDLINIKCLAEQIVEFAAYREQLSNYLSSRMKAIAPNLTQLVGELVGARLIAHSGSLISLAKSPASTIQILGAEKALFRALKTKHDTPKYGLLYHASLVGQASGKNKGKIARVLAAKAAVSLRYDALAEDRDDSGDIGLESRAKVESRLGATSIKQRSLLNMEAGYMTITLKSSVPIHNLSYAWRSIKEQLGEDVDSKIHRMCLLKDSMGVCFDVRSEDLQSMQESWSDTRRWQFTITTELPEIR",
    "response#2": "LLDDLDKELNTYAMRCKEWYGWHFPELAKIVTDSVAYARIILTMGIRVNAAETDMSEILPEEIEERVKTAAEVSMGTEITPVDLINIKCLAEQIVEFAAYREQLSNYLSSRMKAIAPNLTQLVGELVGARLIAHSGSLISLAKSPASTIQILGAEKALFRALKTKHDTPKYGLLYHASLVGQASGKNKGKIARVLAAKAAVSLRYDALAEDRDDSGDIGLESRAKVESRLPFQAVSIACIERQESVLVSAHTSAGKTVVAEYAVAQSLRDKQRVIYTSPIKALSNQKYRELLAEFGDVGLMTGDVTINPDATCLVMTTEILRSMLYRGSEVMREVAWVIFDEIHYMRDKERGVVWEETIILLPDKSHFVFLSATIPNERTLCVHLGLLLGVGGQMQELRRVRSGVMSEKDHMVTMHDVLDAQWLYDNHKDESYLRRVVYPLEKLLYPFTLDPFQAVSIACIERQESVLVSAHTSAGKTVVAEYAVAQSLRDKQRVIYTSPIKALSNQKYRELLAEFGDVGLMTGDVTINPDATCLVMTTEILRSMLYRGSEVMREVAWVIFDEIHYMRDKERGVVWEETIILLPDKSHFVFLSATIPNAMQFAEWITKIHRQPCHVVYTDFRPTPLQHLCRGATSIKQRSLLNMEAGYMTITLKSSVPIHNLSYAWRSIKEQLGEDVDSKIHRMCLLKDSMGVCFDVRSEDLQSMQESWSDTRRWQFTITTELPEIT",
    "response#3": "GATSIKQRSLLNMEAGYMTITLKSSVPIHNLSYAWRSIKEQLGEDVDSKIHRMCLLKDSMGVCFDVRSEDLQSMQESWSDTRRWQFTITTELPEIL"
  },
  {
    "reference": "MERFLVASKNKDKIHEIKEILDGFPFEIIGMESIGIHTDIVEDADSFEGNALIKASTIQKQVGGYVMADDSGLCIDALDGAPGIYSSRFCGEDSTYQEKFEKIWEMLKGVPKDKWTAHFVCAIAVVRPDGSHFVVKEQCDGVLLDSPRGENGFGYDPIFFVPEIGKTTAEISDEEKNAISHRGKALRSMLAILKES",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have XTP diphosphatase activity to facilitate purine nucleoside triphosphate catabolic process, nucleotide metabolic process.2. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.3. The protein with the reaction dITP + H2O = dIMP + diphosphate + H(+), the Proton acceptor must be optimized to ensure efficient catalysis.4. The protein should be able to bind substrate ligand in a variety of conditions5. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions. The designed protein sequence is ",
    "response#1": "MSYVFLATGNQGKVKELGRMIAPLGLTIKAQAAFNVSEVAETGTTFVENAIIKARHAAKVSGLPAIADDSGLEVDHLLGAPGIHSARYAGEPKSDAANNAKLLAALEGVPWEKRTARYVCVLALVRHADDPLPIICQGAWEGTILHEARGEGGFGYDPLFWVPELERTSAELSLEVKNRLSHRGKALQQLLERLPA",
    "response#2": "MQKTIVFATNNAHKLREVQALLSQ",
    "response#3": "MFYSIMFTGRGNSIFYT"
  },
  {
    "reference": "MFWISLVCLSVSHKMIGMCYLLVAILCGFLGYIYSLFIRLELSIIGCGVLFGDYQYYNVLVTTHGLVMVFAFIMPVMMGGLVNYFVPVLSGFPDMVFPRLNNMSFWMFMGGFGALVSGLLTEEGMGIGWTMYPTLICVDFHSSLACDFTVFAVHLLGVSSILNSINLLGTLFCCRRKFFSFLNWTLFIWGSLITALLLIITLPVLAGGVTLVLCDRNFNTSFYDVVGGGDLLLFQHLFWFFGHPEVYIIIIPVFGLISTMVEVLGFRCVFSSVAMIYSMILIAILGFFVWAHHMFVVGMDVDTRAYFGSVTVLIGLPTCIKLFNWTYSFLYTDFIIAFEA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation; Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)); The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix..2. The designed protein should have heme binding, metal ion binding within respirasome, mitochondrial inner membrane to regulate oxidative phosphorylation.3. The protein should contain a Helical membrane-spanning region.4. Cu cation is required for the proper folding and stability of the protein.5. The protein should be able to bind Cu cation, Fe(II)-heme a, O2 ligand in a variety of conditions6. Ideal protein should have a binding affinity for key enzymes in the oxidative phosphorylation, Energy metabolism pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MFWLCLVCLSVSHKMIGLCYLLVAILSGFVGYVYSLFIRLELSLIGCGILFGDYQFYNVLITSHGLIMVFAFIMPVMMGGLVNYFIPVMAGFPDMVFPRLNNMSFWMYLAGFGCVVNGFLTEEGMGVGWTLYPTLICIDFHSSLACDFVMFAVHLLGISSILNSINLLGTLFCCRRKFFSFLSWSLFIWAALITAILLIITLPVLAGGVTLILCDRNFNTSFYDVVGGGDLILFQHIFWFFGHPEVYIILLPVFGLISTIVEVIGFRCVFSTVAMIYSMILIAILGMFVWAHHMFVVGMDVDSRAYFGGVSILIGLPTCVKLFNWIYSFLYTDMIITFEVYFVIMFIFMFLIGAVTGLFLSNVGIDIMLHDTYFVVGHFHYVLSLGAVVGFFTGFIHFLAKWLPIELYLFWMFYFISTLFIGSNMLFFPMHSLGMYAFPRRISDYPVSFLFWSSFMLYGMLLLASLILFLCALFCVFLFWDYCLFFVSLFVFSG",
    "response#2": "MWKERWFLSTNAKDIGTLYLMFRYFSGLVGTAFSVLIRLELSAPGVQYIADNQLYNSIITAHAILMIFFMVMPALIGGFGNFLLPLLVGGPDMAFPRLNNISFWLLIPSLLLFVFASIIENGAGTGWTLYPPLASIQSHSGPSVDLAIFGLHLSGISSLLGAMNFITTIINMRSPGIRLHKLALFGWAVLITAVLLLLSLPVLAGAITMLLTDRNFNTSFFELAGGGDPIFIQHLFWFFGHPEVYILIVPGFGIISTVISANSSKNVFGYLGMVYAMMSIGILGFVFWSHHMYTVGLDVDTRAYFIAATLIIAVPTGIKIFSWLATCYGGSLNLTPAMLFALGFVVMFTIGGLSGVVLANASLDIAFHDTYYVVAHFHYVLSMGAVFALFSGWYFWIPKLLGLSYDLFAGKVHFWILFVGVNLTLFPQHFLGLQGMPRRIGDYPDAFAGWNLISSFGSIVSVVATWYFLNILYLQLTQGSPVSRYPWLTPQYFSDLFQHLFTRNNSSLEWCLNSPPKPHAFDCLPVQS",
    "response#3": "MSRKWLYSTNHKDIGTLYFIFGIWSGMVGTSLSLLIRAELGNPGSLIGDDQIYNTIVTAHAFIMIFFMVMPIMIGGFGNWLVPLMLGAPDMAFPRMNNMSFWLLPPSLTLLISSSIVENGAGTGWTVYPPLSSNIAHGGSSVDLAIFSLHLAGITSILGAINFITTIINMRINGMSFDQMPLFVWSVGITALLLLLSLPVLAGAFTMLTTDRNLNTSFFDPAGGEILFYIQHLFWFFGHPEVYILILPGFGMISMLFHKKEEKKKHFGSLGMIYAMMAIGLLGFVVWAHHMFTVGMDIDTRAYFTSATMIIAVPTGIKIFSWLATLHGTQINYSPSILWSLGFVFLFTVGGLTGVVLANPSIDIALHDTYYVVAHFHYVLSMGAVFAIIAGFIHWYPLFTGLSLNPYFLKIQFFTMFIGVNLTFFPQHFLGLAGMPRRYSDYPDAYISWNIISSLGSYISLLAVMLILIIIWESMINQRMILFSLNLTSSMEWYKSKLPPAEHSYNELPILKT"
  },
  {
    "reference": "MKHLASSIALTLLLPAVQAQQTVWGQCGGQGWSGPTNCVAGAACSTLNPYYAQCIPGATATSTTLSTTTTTQTTTKPTTTGPTTSAPTVTASGNPFSGYQLYANPYYSSEVHTLAMPSLPSSLQPKASAVAEVPSFVWLDVAAKVPTMGTYLADIQAKNKAGASPPIAGIFVVYDLPDRDCAALASNGEYSIANNGVANYKAYIDAIRAQLVKYSDVHTILVIEPDSLANLVTNLNVAKCANAQSAYLECVDYALKQLNLPNVAMYLDAGHAGWLGWPANLGPAATLFAKVYTDAGSPAALRGLATNVANYNAWSLSTCPSYTQGDPNCDEKKYINAMAPLLKNAGFDAHFIMDTSRNGVQPTKQSAWGDWCNVIGTGFGVRPSTNTGDPLQDAFVWIKPGGESDGTSNSSSARYDAHCGYSDALQPAPEAGTWFQAYFEQLLTNANPSF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A key design requirement for the protein is the optimization of the active site, Nucleophile to achieve high catalytic efficiency for Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose   and cellotetraose, releasing cellobiose from the non-reducing ends of   the chains..2. The protein should be optimized for stability and expression in extracellular region where it will participate in cellulose catabolic process.3. Incorporate a signal peptide in the protein design.4. For general function, the protein need meet that The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose. The designed protein sequence is ",
    "response#1": "HATVFAVWINDEDQGLGNTADGYIRTPPNNSPVTDVTSTDLTCNVNGDQAAAKTLEVAAGDKITFEWHHNSRDSSDDIIADSHKGPVLVYMAPTEAGSAGKNWVKIYEDGYNDGTWAVDTLIANKGKHSVTVPDVPAGNYLFRPEIIALHEGNREGGAQLYMECVQFKVTSDGTTQLPEGVSLPGAYTATDEGILFDIYSSFDSYPIPGPAVHATVFAVWINDEDQGLGNTADGYIRTPPNNSPVTDVTSTDLTCNVNGDQAAAKTLEVAAGDKITFEWHHNSRDSSDDIIADSHKGPVLVYMAPTEAGSAGKNWVKIYEDGYNDGTWAVDTLIANKGKHSVTVPDVPAGNYLFRPEIIALHEGNREGGAQLYMECVQFKVTSDGTTQLPEGVSLPGAYTATDEGILFDIYSSFDSYPIPGPAVTPGEEFYVEAAVNAAGPGFVNIKASIINKSGWPARGSDKLSAKYFVDISEAVAKGITLDQITVQSTTNGGAKVSQLLPWDPDNHIYYVNIDFTGINIFPGGINEYKRDVYFTITAPYGEGNWDNTNDFSFQGLEQGFTSKKTEYIPLYDGNVRVWGKVPHATVFAVWINDEDQGLGNTADGYIRTPPNNSPVTDVTSTDLTCNVNGDQAAAKTLEVAAGDKITFEWHHNSRDSSDDIIADSHKGPVLVYMAPTEAGSAGKNWVKIYEDGYNDGTWAVDTLIANKGKHSVTVPDVPAGNYLFRPEIIALHEGNREGGAQLYMECVQFKVTSDGTTQLPEGVSLPGAYTATDEGILFDIYSSFDSYPIPGPAV",
    "response#2": "ELERCVGQTGSVPRLGIPSLCLQDSPLGIRMSDYNSAFPAGINVAATWDKKLAYQRGKAMGEEFSDKGIDVQLGPAAGPLGRSPDGGRNWEGFSPDPALTGVLFAETIKGIQDAGVIATAKHYILNEQEHFRQVGEAQGYGFNITETVSSNVDDKTMHELYLWPFADAVRAGVGAVMCSYNQINNSYGCQNSLTLNKLLKAELGFQGFVMSDWSAHHSGVGAALAGLDMSMPGDISFDSGTSFYGTNLTVGVLNGTIPQWRVDDMAVRIHATVFAVWINDEDQGLGNTADGYIRTPPNNSPVTDVTSTDLTCNVNGDQAAAKTLEVAAGDKITFEWHHNSRDSSDDIIADSHKGPVLVYMAPTEAGSAGKNWVKIYEDGYNDGTWAVDTLIANKGKHSVTVPDVPAGNYLFRPEIIALHEGNREGGAQLYMECVQFKVTSDGTTQLPEGVSLPGAYTATDEGILFDIYSSFRCKYTDDISNYVNNEVACDYNAHATVFAVWINDEDQGLGNTADGYIRTPPNNSPVTDVTSTDLTCNVNGDQAAAKTLEVAAGDKITFEWHHNSRDSSDDIIADSHKGPVLVYMAPTEAGSAGKNWVKIYEDGYNDGTWAVDTLIANKGKHSVTVPDVPAGNYLFRPEIIALHEGNREGGAQLYMECVQFKVTSDGTTQLPEGVSLPGAYTATDEGILFDIYSSFRCK",
    "response#3": "ELERCVGQTGSVPRLGIPSLCLQDSPLGIRMSDYNSAFPAGINVAATWDKKLAYQRGKAMGEEFSDKGIDVQLGPAAGPLGRSPDGGRNWEGFSPDPALTGVLFAETIKGIQDAGVIATAKHYILNEQEHFRQVGEAQGYGFNITETVSSNVDDKTMHELYLWPFADAVRAGVGAVMCSYNQINNSYGCQNSLTLNKLLKAELGFQGFVMSDWSAHHSGVGAALAGLDMSMPGDISFDSGTSFYGTNLTVGVLNGTIPQWRVDDMAVRIHATVFAVWINDEDQGLGNTADGYIRTPPNNSPVTDVTSTDLTCNVNGDQAAAKTLEVAAGDKITFEWHHNSRDSSDDIIADSHKGPVLVYMAPTEAGSAGKNWVKIYEDGYNDGTWAVDTLIANKGKHSVTVPDVPAGNYLFRPEIIALHEGNREGGAQLYMECVQFKVTSDGTTQLPEGVSLPGAYTATDEGILFDIYSSFGSYVLGRNALNMSYVTGYGEVSSHNQHSTTHATVFAVWINDEDQGLGNTADGYIRTPPNNSPVTDVTSTDLTCNVNGDQAAAKTLEVAAGDKITFEWHHNSRDSSDDIIADSHKGPVLVYMAPTEAGSAGKNWVKIYEDGYNDGTWAVDTLIANKGKHSVTVPDVPAGNYLFRPEIIALHEGNREGGAQLYMECVQFKVTSDGTTQLPEGVSLPGAYTATDEGILFDIYSSFDSYPIPGPAVPTNDEFFVEAGINASGTNFIEIKAIVNNQSGWPARATNKLKFRYFVDLSELIKAGYSPNQLTLSTNYNQGAKVSGPYVWDSSRNIYYILVDFTGTLIYPGGQDKYKKEVQFRIAAPQNVQWDNSNDYSFQDIKGVSSGSVVKTKYIPLYDEDIKVWGEEP"
  },
  {
    "reference": "MAPRALWSCYLCCLLTIATEAASYPPRGYSLYTGGTGALSPGGPQAQNSPRPASRHRNWCAYVVTRTVSCVLEDGVETIVKPDYQPCGWGQPHCSRSIMYRSFLRPRYRVAYKTVTDMEWRCCQGYGGDDCGEGPASVLGPAPSTPLPRPRPVRPNLSGSSAGSHLSGLGGEGPVESEKVQQLERQVKSLTKELQGLRGVLQGMNGRLAEDVQRAVDTVFNGRQQPADAAARPGVHETLSEIQQQLQLLDNRVSTHDQELGHLNNHHNGGPGGGGRASGPVPVPSGPSEELLRQLERQLQESCSVCLTGLDGFRQQQQEDRERLRTLEKLMSSMEERQQQLVGPAMARRPPQECCPPELGRRVSELERRLDVVTGSLTVLSGRRGSELGGAAGQGGHPPGYTSLASRLSRLEDRFNSTLGPSEEQEKNWPGGPGRLGHWLPAAPGRLEKLEGLLANVSRELGGRMDLLEEQVAGAVRTCGQICSGAPGEQDSRVNEILSALERRVLDSEGRLQLVGSGLHEAEAAGEAQQAVLEGLQGLLSRLRERMDAQEETAAEILLRLNLTAAQLSQLEGLLQARGDEGCGACGGVQEELGRLRDGVERCSCPLLPPRGPGAGPGVGGPSRGPLDGFSVFGGSSGSALQALQGELSEVILTFSSLNDSLHELQTTVEGQGADLADLGATKDSIISEINRLQQEATEHVTESEERFRGLEEGQAQAGQCPSLEGRLGRLEGVCERLDTVAGGLQGLREGLSRHVAGLWAAVRESNS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. Incorporate a signal peptide in the protein design.2. The presence of C1q, Collagen-like, EMI domains is critical for the interaction of the designed protein with its intended targets.3. A protein that contributes to aortic valve morphogenesis by functioning in extracellular matrix, EMILIN complex, extracellular region with identical protein binding, extracellular matrix constituent conferring elasticity.4. Ensure the presence of a signal peptide within the protein.5. The protein must exhibit the following characteristics: May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly; Has cell adhesive capacity . The designed protein sequence is ",
    "response#1": "ILIANSGFQVPQGRSVWLNPLRDLVIRVQRGDQCKVTVLDFPRLQGALSPHQFPCDFGAQQVKYTHFGSPSSTRTRIQLQVRYDAGNSTLVLPFTLQVNVVFPKLQLVARNRPLKVFKVLGCSHAIDRRVLNFASRSRCRLTVLPHPGGPLPKYGLLVDTAGNPLSRGHLADCEAFIQAGVRYQHTTTPSSPSRDYVPMLF",
    "response#2": "ILIANSGFQVPQGRSVWLNPLRDLVIRVQRGDQCKVTVLDFPRLQGALSPHQFPCDFGAQQVKYTHFGSPSSTRTRIQLQVRYDAGNSTLVLPFTLQVNVVFPKLQLVARNRPLKVFKVLGCSHAIDRRVLNFASRSRCRLTVLPHPGGPLPKYGLLVDTAGNPLSRGHLADCEAFIQAGVRYQHTTTPSSPSRDYVPMLF",
    "response#3": "ILIANSGFQVPQGRSVWLNPLRDLVIRVQRGDQCKVTVLDFPRLQGALSPHQFPCDFGAQQVKYTHFGSPSSTRTRIQLQVRYDAGNSTLVLPFTLQVNVVFPKLQLVARNRPLKVFKVLGCSHAIDRRVLNFASRSRCRLTVLPHPGGPLPKYGLLVDTAGNPLSRGHLADCEAFIQAGVRYQHTTTPSSPSRDYVPMLSS"
  },
  {
    "reference": "MEIRAAEISKVIKDQIANFGTDATVTEIGSVLSVGDGIARVHGLDNVQAGEMVEFANGVKGMALNLEADNVGIVIFGSDAAIKEGDQVKRTGTIVDVPVGKGLLGRVVDGLGNPIDGKGPIKADKRMRVEVKAPGIIPRTSVHEPVQTGLKALDALVPVGRGQRELIIGDRQTGKTAVAIDTFINQKAANAGDDESKKLYCIYVAVGQKRSTVAQIVRQLEENGAMEYSIVVAATASEPAPLQFLAPYTGCTMGEFFRDNGMHAVIVYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNSDNGSGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLETNLFNAGIRPAINVGLSVSRVGSAAQTKAMKKVSGSIKLELAQYREMEAFAQFGSDLDASTQKLLGRGARLTQLLKQPQFQPMPFEEQTASIFAGTNGYLDTVATTDVSRYEAAMLAYLRSDHPQVLAAIRDSKDLGDDTKKALVAALDAFAKIFA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Produces ATP from ADP in the presence of a proton gradient across the membrane; The alpha chain is a regulatory subunit..2. The protein should have ATP binding, proton-transporting ATP synthase activity, rotational mechanism and be active in proton-transporting ATP synthase complex, catalytic core F(1), plasma membrane.3. The ATP binding site should be stable and able to withstand changes in environmental conditions.4. The protein should be designed to catalyze the following reaction ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate. The designed protein sequence is ",
    "response#1": "MKKVAGTLKIDQAQFRELESFSKFGGEMDAVTAFTIDKGQKNTQLLIQPQYSPMPVEEQISILYCGTQGLLKEVPLDKVHEFEARFLQELRTSHQRDVLDVLKTGVINDEIRGILEKTAKEE",
    "response#2": "MKKVAGTLKIDQAQFRELESFSKFGGEMDAVTAFTIDKGQKNTQLLIQPQYSPMPVEEQISILYCGTQGLLKEVPLDKVHEFEARFLQELRTSHQRDVLDVLKTGVINDEIRGILEKTAKEA",
    "response#3": "MKKVAGTLKIDQAQFRELESFSKFGGEMDAVTAFTIDKGQKNTQLLIQPQYSPMPVEEQISILYCGTQGLLKEVPLDKVHEFEARFLQELRTSHQRDVLDVLKTGVINDEIRGILEKTAKEA"
  },
  {
    "reference": "MTSSRPKTRFIFITGGVVSSLGKGIAAASIGRLLVARGLSVGLQKFDPYINVDPGTMSPYQHGEVFVTEDGAETDLDLGHYERYTDANTSRASNVTAGGIYNSVIRRERRGDYLGGTVQVVPHITDEIKQRVQLLAQSQDVDVVITEIGGTVGDIESLPFLEAIRQFPVDVGRRQCMFIHLTLVPYIGHAGELKTKPTQHSVNELRRIGIQPDMLLARSESELGTDIRKKIALFASLPVEAVVSAKDVDNIYKVPLWFETEGVDDLICEHFGFDVPPPDLSEWAEITRRASAVSESVKIALVGKYVALQDAYLSVSEALRHSGFHHDCAIDVDWVDSEALTDDETARRRLGDADGILIPGGFGGRGIEGKIRAAMVAREQQIPYLGVCLGMQVAVMEFARHIAGMDGANSTEFDPETPHPVIDLLPEQKEVTDLGGTMRLGADPVKIHATTRARELYGEAVIYERHRHRYEVNLFLRKRLEAAGLIVSGTSPDERLVEMIELDGHPFFVASQFHPEFKSRPERPAPLFREFVGAALRRAREGQDEAGGSGEVEPEFRVLRS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. We require a protein that can specifically bind to the metabolites produced in the Pyrimidine metabolism, CTP from UDP: step 2/2, CTP biosynthesis via de novo pathway pathway.2. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates..3. The protein with the reaction H2O + L-glutamine = L-glutamate + NH4(+), the Nucleophile; for glutamine hydrolysis, active site, Nucleophile must be optimized to ensure efficient catalysis.4. The protein should have a high affinity for CTP, Mg(2+), UTP, ATP.5. The presence of Glutamine amidotransferase, CTP synthase N-terminal domains is critical for the interaction of the designed protein with its intended targets.6. The designed protein must be able to perform metal ion binding, CTP synthase activity, ATP binding under specific conditions. The designed protein sequence is ",
    "response#1": "KDAYKSLSEALRHGGLKQNTQVNLRWVESEEIEARGAEMLRDVDGILVPGGFGKRGFEGKIAAARYARENSIPYFGICYGMHAAVVDFARSIAGLPGANSSENDRLAKDPVIALITEWTKSSGEIEHRSEDSDLGGTMRLGAQEAKLKPGTLARELYGQDVIRERHRHRYEFNNNYRQKFADLGLVISGVSMDEELVEMIEFSPQKHPWFVAGQFHPEFTSTPRDGHPLFFGFIKSQYKIK",
    "response#2": "LVDSYKSIAEAFIHGGAVNDVEVDVEWRSAEDVEAGGTDFLERFHGVLIPGGFGERGIDGMIDTIRFVREREMPYFGICLGLQCAIIEFARNVCDLPTAHSTEFDPGTSDPVISLLDSQHQVTDMGGTMRLGAYPCLLQPGSRVHGVYGADEISERHRHRYEVNPAYRETLGAKGLVFSGMSPDGGLVEMLELPEHPYFLATQFHPELKSRPTKAHPLFAAFVEAAAKLLG",
    "response#3": "KFIFITGGVLSALGKGIAAASIGMLLESHGYKITIQKFDPYLNVDPGTMNPFQHGEVYVTDDGAETDLDLGHYERFTSTKTTKAHNWTSGKIYESIIRKERKGEYLGKTVQVIPHVTDEIKDAFYAVADKNDIVIVEIGGTVGDIESQPFLEAIRQMRLSLGSDNTLFIHLTLVPFIKTSGELKTKPTQHSVRELQAAGIQPDIILCRTDMFLSQDIKAKISLFTNVPLEAVITAKDVDNIYEVPLVLAQEALDSIILKKFNLEYKQEPDLNKWKEFVNRIKNPKHELQDSYKSILESFIHAGAANQVKVNVRFIHSEHLNMGNIEKKLAGLNGILVAPGFGERGIEGKIKAVRYTRENKIPFLGICLGMQMAVIEYSRTVLGLSKANSTEMNEKTTAPVINLMEEQKSVTNKGGTMRLGAWDCTLKKGSLASKTYQGASDISERHRHRYEFNNDYKEQLEDAGMLATGINKETGLVEIVEVPEHPWFVGVQYHPEYKSTVANPHPLFVGFIFLRCMM"
  },
  {
    "reference": "MLCHVTRPDAVVMEIEVDAKANGEDCLNKVCRKLGIIEVDYFGLQFSGSKGENLWLNLRNRISQQMDNLTPCRLRLRVKFFVEPHLILQEQTRHLFFMHVKEDLHRGHLRMCSEQAQELSALLAQAEFGDYNQNTAKYWYTELCGSEPNQTTINSIIAKHKALEGLSQASVEYQALQLVSSLEHYGVEWHWARDAEAQRLAIGVGPEGIAICRDDFSLVNRISYPIIQIATQSGKSVYLTVTKESSDSVVLLFKLISNRAASGLYRAITETHAFYRCDTVTNAVMMQYSRDFKGHLASLFLNENINLGKKYVFDIRRTSKEVYDYARRALYNAGIVDMMSRPGERTPSNRSPSREQEGALDCGGCQQSRLLQEKLQKLREALLCMLCCEEEIDAAFCPCGHMVCCQNCAAQLQSCPVCRSEVEHVQHVYLPTCTSLLNLTIGENSPEPIHRGMAAHTCTTNDYSTSEKIYQN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein can be expressed in cytosol, cytoskeleton and perform ubiquitin-protein transferase activity without triggering undesirable cellular responses.2. The protein contains novel FERM domains that confer a unique function or activity.3. The protein must exhibit the following characteristics: Regulates cell movements during gastrulation by acting downstream of fz7 to antagonize the frizzled-signaling pathway.; E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC) .4. A protein that is capable of catalyzing reactions within the Protein modification pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MPGNPYSSNEPGIGPLMRDIKNKICQDCDLVALLEDDSGMELLVNNKIISLDLPVAEVYKKVWCATNEGEPMRIVYRMRGLLGDATEEFIESLDSTTDEEEDEEEVYRMAGVMAQCGGLQCMLNRLAGVKDFKQGRHLLTVLLKLFSYCVKVKVNRQQLVKLETNTLNVMLGTLNLALVAEQESKDSGGAAVAEQVLSIMEIILDESNAEPLSEDKGNLLLTGDKDQLVMLLDQINSTFVRSNPSVLQGLLRIIPYLSFGEVEKMQILVERFKPYCSFEKYDEDHSGDDKVFLDCFCKIAAGIKNNSNGHQLKDLILQKGITQNALDYMKKHIPSAKNLDADIWKKFLSRPALPFILRLLRGLAMQHPATQVLIGTDSITSLHKLEQVSSDEGIGTLAENLLEALREHPDVNKKIDAARRETRAEKKRMAMAMRQKALGTLGMTTNEKGQVVTKTALLKQMEELIEEPGLTCCICREGYKFQPTKVLGIYTFTKRVALEEMENKPRKQQGYSTVSHFNIVHYDCHLAAVRLARGREEWESAALQNANTKCNGLLPVWGPHVPESAFATCLARHNTYLQECTGQREPTYQLNIHDIKLLFLRFAMEQSFSADTGGGGRESNIHLIPYIIHTVLYVLNTTRATSREEKNLQGFLEQPKEKWTESAFDVDGPHYFTILALHVLPPEQWKAIRVEILRRLLVASHARAVAPGGATRLTDKAVKDYSAYRSSLLFWALVDLIYNMFKKVPTSNTEGGWSCSLAEYIRHNDMPIYEAADKALKTFQEEFMPVETFSEFLDAA",
    "response#2": "MPGNPYSSNEPGIGPLMRDIKNKICQDCDLVALLEDDSGMELLVNNKIISLDLPVAEVYKKVWCATNEGEPMRIVYRMRGLLGDATEEFIESLDSTTDEEEDEEEVYRMAGVMAQCGGLQCMLNRLAGVKDFKQGRHLLTVLLKLFSYCVKVKVNRQQLVKLETNTLNVMLGTLNLALVAEQESKDSGGAAVAEQVLSIMEIILDESNAEPLSEDKGNLLLTGDKDQLVMLLDQINSTFVRSNPSVLQGLLRIIPYLSFGEVEKMQILVERFKPYCSFEKYDEDHSGDDKVFLDCFCKIAAGIKNNSNGHQLKDLILQKGITQNALDYMKKHIPSAKNLDADIWKKFLSRPALPFILRLLRGLAMQHPATQVLIGTDSITSLHKLEQVSSDEGIGTLAENLLEALREHPDVNKKIDAARRETRAEKKRMAMAMRQKALGTLGMTTNEKGQVVTKTALLKQMEELIEEPGLTCCICREGYKFQPTKVLGIYTFTKRVALEEMENKPRKQQGYSTVSHFNIVHYDCHLAAVRLARGREEWESAALQNANTKCNGLLPVWGPHVPESAFATCLARHNTYLQECTGQREPTYQLNIHDIKLLFLRFAMEQSFSADTGGGGRESNIHLIPYIIHTVLYVLNTTRATSREEKNLQGFLEQPKEKWTESAFDVDGPHYFTILALHVLPPEQWKAIRVEILRRLLVASHARAVAPGGATRLTDKAVKDYSAYRSSLLFWALVDLIYNMFKKVPTSNTEGGWSCSLAEYIRHNDMPIYEAADKALKTFQEEFMPVETFSEFLDAA",
    "response#3": "MPGNPYSSNEPGIGPLMRDIKNKICQDCDLVALLEDDSGMELLVNNKIISLDLPVAEVYKKVWCATNEGEPMRIVYRMRGLLGDATEEFIESLDSTTDEEEDEEEVYRMAGVMAQCGGLQCMLNRLAGVKDFKQGRHLLTVLLKLFSYCVKVKVNRQQLVKLETNTLNVMLGTLNLALVAEQESKDSGGAAVAEQVLSIMEIILDESNAEPLSEDKGNLLLTGDKDQLVMLLDQINSTFVRSNPSVLQGLLRIIPYLSFGEVEKMQILVERFKPYCSFEKYDEDHSGDDKVFLDCFCKIAAGIKNNSNGHQLKDLILQKGITQNALDYMKKHIPSAKNLDADIWKKFLSRPALPFILRLLRGLAMQHPATQVLIGTDSITSLHKLEQVSSDEGIGTLAENLLEALREHPDVNKKIDAARRETRAEKKRMAMAMRQKALGTLGMTTNEKGQVVTKTALLKQMEELIEEPGLTCCICREGYKFQPTKVLGIYTFTKRVALEEMENKPRKQQGYSTVSHFNIVHYDCHLAAVRLARGREEWESAALQNANTKCNGLLPVWGPHVPESAFATCLARHNTYLQECTGQREPTYQLNIHDIKLLFLRFAMEQSFSADTGGGGRESNIHLIPYIIHTVLYVLNTTRATSREEKNLQGFLEQPKEKWTESAFDVDGPHYFTILALHVLPPEQWKAIRVEILRRLLVASHARAVAPGGATRLTDKAVKDYSAYRSSLLFWALVDLIYNMFKKVPTSNTEGGWSCSLAEYIRHNDMPIYEAADKALKTFQEEFMPVETFSEFLDAA"
  },
  {
    "reference": "MTGELQGRRVLLIIGGGIAAYKSLDLIRRLRDAGGQVRCVMTAGAQEFVTPLAAAALSGEKTWTDLFDRADEAAIGHIRLARDADCVVVAPATANLMARMAQGHADDLATTVLLATRLPVLIAPAMNPAMWAHPATQRNLATLKRDGVRTVGPDSGPMAEPETGAGRMAEPMAIVAATQALFDVAQTPRDGALAGRHVLVTSGPTHEPIDPVRYIANRSSGRQGHAIAAAAARAGARVTLVSGPVGIPAPPGVDLVPVETARQMQAAVDAALPADIAIFAAAVADWRTDRDAARKIKKVGGGPPALALVENPDILATVAAMRAGRPALVVGFAAETHDVIALARAKRARKGCDLMIANDVSAGSGVAGGVMGGARNAVHLIDAGDAVISWPEMDKDMVAERLIAECVTRLEGLSEADLDAGSQA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. We are looking for a protein with broad substrate specificity for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.2. The designed protein should have a binding site that is specific to the target CTP ligand.3. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.4. The designed protein should interact with enzymes and substrates within the CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis, Cofactor biosynthesis pathway to enhance or regulate its activity.5. The designed protein should contain one or more DNA/pantothenate metabolism flavoprotein C-terminal domains that are essential for its function.6. The designed protein must possess phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity, FMN binding to support coenzyme A biosynthetic process, pantothenate catabolic process under certain conditions. The designed protein sequence is ",
    "response#1": "MQIVLGVAGGIAAYKAVLLLRLLREAGHDVRVVPTRAALEFVGRPTWEALSGQSATPEVFDDVAHVPHVALGQHADLVIVAPATADLLARAAAGRADDLLTSTLLTARCPVLMAPAMHTEMWDHPATRANVATLRARGVHVLDPASGRLTGADTGPGRLPEPDQIAAAALAMLRPAGPALSGRHVVVSAGGTREPLDPVRYLGNRSSGKQGYALARTAAARGARVTLVAANTALPDPAGVRVVPVGTAVELRERVLEAAGDADAVVMAAAVADFRPAAYATGKIKKQEGRDPEPVALVRNPDVLAEVAGDRTRPGQVVVGFAAETDDVLANGRAKLVRKGCDLLVVNEVGERRTFGAEENEAVVLGADGSRILAGRHVVISAGGTREPLDPVRYLGNRSSGRQGYALARTARDRGARVTVVAANVSLDDPAGVDVVEVETTDQLRTAVRAAARTADVVVMAAAVADFRPVAAAGHKVKKRSDGVVDPILLEANPDILAELTAERLRPGQVIVGFAAETGDEHTTVLEYGRAKARRKGADLLAVNAVGHGLAFGTTDNAVTILGADGSRVESAAGSKMQVADALWDAVRERLGS",
    "response#2": "MQIVLGVAGGIAAYKAVLLLRLLREAGHDVRVVPTRAALEFVGRPTWEALSGQSATPEVFDDVAHVPHVALGQHADLVIVAPATADLLARAAAGRADDLLTSTLLTARCPVLMAPAMHTEMWDHPATRANVATLRARGVHVLDPASGRLTGADTGPGRLPEPDQIAAAARELLAAGERPLAGTRVLVSAGGTREPLDPVRYLGNRSSGRQGWALARVAAARGADVTLVAANVEDTAPFGCRVVPVGSAGDLRSAMAEHAPAADVVVMAAAVADFRPTAVADTKMKKGSGSEPTAVALTRNADVLAELVAARPEGQLVVGFAAETGDADDDVLGHARAKLARKGCDLLVVNDVSAGQVFGRPDNAVTVVGVDGEVVLGVSGGIAAYKACELLRRLTESGHAVTVVPTASALRFVGEPTWAALSGRPAATEVWETVHEVPHVRIGQRADLVVVAPATADLLAKAAHGMADDLLTNTLLTARCPVLFVPAMHTEMWEHPATRENVATLRRRGCVVLEPGVGRLTGADTGRGRLPDPDQIFQACRAVLDLARSRR",
    "response#3": "MQIVLGVAGGIAAYKAVLLLRLLREAGHDVRVVPTRAALEFVGRPTWEALSGQSATPEVFDDVAHVPHVALGQHADLVIVAPATADLLARAAAGRADDLLTSTLLTARCPVLMAPAMHTEMWDHPATRANVATLRARGVHVLDPASGRLTGADTGPGRLPEPDQIAAAARTVLGAVDDLAGTRVLVSAGGTREPLDPVRYLGNRSSGRQGWALARVAAARGADVTLVAANVEDTAPFGCRVVPVGSAGDLRSAMAEHAPAADVVVMAAAVADFRPTAVADTKMKKGSGSEPTAVALTRNADVLAELVAARPEGQLVVGFAAETGDADDDVLGHARAKLARKGCDLLVVNDVSAGQVFGRPDNAVTVELLGADGTRAEVPHASKRALADVVWDLVVARLGG"
  },
  {
    "reference": "MQPVLTAIEMQQADSAAIEKLHISPICLMELAGKECIGYMLEKLSLESPEGLQFLIVAGKGNNGGDGFVIARHLLNLGAAVDLVLLYPEGAAGGAAEANMSILRAYEEEGLPLRIFNSIEEAIPFVSEQEYSVTIDAMTGTGLRLEAPGAPLLSPLAEGVELLNALHERSEALCMAVDIPTGLDATSGHAAMPAVTADFTITMGFMKTGLLLQDGPELCGEVHVAEISIPGFLAEDAGAFLIDESFAAEHFALRSEGSAKHMNGKVLIIAGSSTGESSMGGAALLAAGAAVKTGAGYVAVSIPPSLAATLHIAVPEAIVIGRDMDSLMEKARWSEAILIGPGLGRSPESIDLVRALLSHPDIFEKKLILDADALYAIAEAGLQNVLSAMPEVLITPHYGELARLTGTDAGSVTKDPLAAATRFADTYSTTVLLKGRPTIIAKESGPVLLNTSGTEALATAGTGDVLSGMVAALAAKGECLPEAAAAAAWFHGRAGDLASDVSSLVSASMVRDAIPSAIEEMFDFED",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.2. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration3. A protein with NADHX epimerase activity, metal ion binding is needed to enhance nicotinamide nucleotide metabolic process in a specific context.4. The designed protein should be able to compete with endogenous proteins or inhibitors for (6S)-NADPHX, K(+) binding. The designed protein sequence is ",
    "response#1": "MQEMDRKTIQELGIPGIVLMENAGRGAAHTFLSHFSPEQGARVIIICGRGNNGGDGYVVGRYLHEAGLRPTIMVLSEKEKISGDARMNLDIIHGMGLDIRYLPNEQSWQDQKKELTHCNYLVDAILGTGLNSPVRGYYGAVIDEINSSGKPVMAIDIPSGLNADNGAIMGLAVRADLTVTFGLPKLGQLVFPGCDMVGRLVRIDICIPHSVAVGEGDLLLLKSPQKDSHKGQNGSVLIIGSNPDYIGAVVFAAQAALSQRMDLVYIIAPEASSNIIKQYNPEFIVRGVPGDTLTMDAYDAIMQLEKRVDSILIGSGSGTDEKTGELFNNILSSTEKPLVIDADALKLISPEMLSQNMVVTPHAHEFKQLFGMEVPEKIDERVEMLMNLSGDYGCQILLKGVTDVITSPEDYKLNRTGNQGMTKGGTGDLLAGLTLALSTKNTLFESAFLSAFILGSACDKLLEEKGLDYGIRDILKKLEGKL",
    "response#2": "MLLCDRLTSAEYNIPGLELMENAGRGTVAAMARHFGGLNERKVLIFTGPGNNGGDGLVIARLLLEQGARPQVVSLVPLEKLQGDAARNLDRVKQLAMPLYTCLDEEELSWVERLVGESDLLVDALFGTGLRSPVTGVFAAVIRCLNASEVPVVAVDIPSGLAGDTGIPQGVSVRARLTCTYGLPKFAQVEPPGKEYVGLLEVIDIGIPAEVVGPSVFGDAFRAPDEEGHKYGRGHALVVSGGVEATGAPRLAARCALRIGAGLVTVASPSDALAVHAAALTAVMVRRADTPADLAALLADRRRNAVALGPALGVGAETRERVATALRSGAHVVLDADALTSFEGEADALAGLVGERPERGVVVTPHTGEFARLCRGRADIGEAESKLAAARAAAAALGVVVVLKGPDTVVAAPDGRAAINANGTPWLGTAGSGDSLTGMTAGLLAQSVPAYEAACAAVWLHAEAGARIGPGLIAEDLSEALPAVLREYFDEEGHKYGRGHALVVSGGVEATGAPRLAARCALRIGAGLVTVASPSDALAVHAAALTAVMVRRADTPADLAALLADRRRNAVALGPALGVGAETRERVATALRSGAHVVLDADALTSFEGEADALAGLVGERPERGVVVTPHTGEFARLCRGRADIGEAESKLAAARAAAAALGVVVVLKGPDTVVAAPDGRAAINANGTPWLGTAGSGDSLTGMTAGLLAQSVPAYEAACAAVWLHAEAGARIGPGLIAEDLSEALPAVL",
    "response#3": "TGKWAKDALPERPLNANKGTFGKVLTVAGSVNYSGAAYLACSGAMRAGAGLVTLAVPVNLQPVLAGKLNEVTYLPLSESVKGVVSGEASFIVLSSLHEYTTLLIGCGLGQHDMTVSFTKGILFGNKPIMPLVIDADALNVLAKTNDWWQKLMMDAILTPHPGEMSRLTGIKIEEIQSNRIDTAMLFAQKWGKTVVLKGAYTVIASADGRCRVSPFANPGMASAGTGDVLAGIIAGLTAQGLDNFNAACLGVWLHGQAGEIVKAELGDTGMLAGDLLQVVPKAIKKE"
  },
  {
    "reference": "MSASLIVLAAGQGTRMQSDLPKVLHELGGVPLFAHAVASGRALEPERVVVVGGHGFAAVEKVAKALDDEIRVVQQAEQLGTAHAVAQAAPALDGFVGDVVVLYGDTPFIRPETLTAMREAREAGADIVVLGFEAEDPGRYGRLVVEGGALTRIVEFKDATEAERAITLCNSGVVMASAATLFGCIDRVGNDNAAGEYYLTDIVEVARAQGLVAKAVSCPEAETLGINTRAELAAAEAAFQSRARAEAMANGVTLVAPETVFFAQDTFIGRDALVEPNVVFGPGVTVETGARIRAFSHLEGCHVSQGAVVGPYARLRPGAELAGGAKVGNFVEIKNARLDEGAKVNHLSYVGDAFVGAGANVGAGTITCNYDGVFKHHTHIGAQSFIGSNTLLVAPVKVGDGAMTATGTVVTGDVPDGAMGIARVPMEVRDGLWTRMYEKMKARKAKLKKQDR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for UDP-N-acetyl-alpha-D-glucosamine binding.2. The protein should have a low Km value for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.3. The protein design must be able to perform glucosamine-1-phosphate N-acetyltransferase activity in order to contribute to lipid A biosynthetic process, regulation of cell shape, cell wall organization, peptidoglycan biosynthetic process.4. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc); The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain..5. The designed protein must have at least one MobA-like NTP transferase domain. The designed protein sequence is ",
    "response#1": "MENGVTLPAPETVHFAFDTAIGRDTVIEQNVVFGPGVTIETGAHIRAFSHLEGCHVSRGAVVGPYARLRPGAELAEDTRIGNFVEVKNATIDEGAKVNHLSYIGDAHVGSGSNIGAGTITCNYDGVGKHHTEIGENVFIGSNTMLVAPVTVGNGALTGSGSVITKDVPADALAIARSQGPVTREQLLAIILAAGKGTRMKSDLHKVLHPIAGRPMLAHLMDSVDRLGPAAKVVVTGAGREQVEELVTTYEGVTAYQAEQLGTAHAVLQARESLERFQGDIIILYGDVPLVGTETMARLIARLHAPDEPALGIVHLEVDDPTGYGRIVRGPDGAVQRIVEHKDASDAERAIREVNTGILAAPTARLKQWLDSLSNDNAQREYYLTDIMENGVTLPAPETVHFAFDTAIGRDTVIEQNVVFGPGVTIETGAHIRAFSHLEGCHVSRGAVVGPYARLRPGAELAEDTRIGNFVEVKNATIDEGAKVNHLSYIGDAHVGSGSNIGAGTITCNYDGVGKHHTEIGENVFIGSNTMLVAPVTVGNGALTGSGSVITKDVPADALAIARSQQVQLPGWQRPTKKR",
    "response#2": "VVIMAAGKGTRMKSALPKVLHKLAGRSLLQHVLSTVSALGPQQRTVVITGHGAEAVEAAVTAPGLQFVRQQPQLGTGHAIQQVSPALDTALDTTLILNGDVPLIRAETAQALVQACGEAVGLLTTRLADPTGYGRILRDAQGRVAAIVEQKDADDAQRAIDEVNTGILVAPTQRLRAWLGRLDNDNAQGEYYLTDVVALAVAEGVAVETAEPADEWETLGVNSRVQQAQLERIHQRNVAAALLEQGVRLADPARFDLRGRLGCGQDVDIDVGCVFEGEVTLGDGVRVGPYCVIRDARIDAGTQLAAFTHIDGAQVGAGAQIGPFARLRPGAQLGDEVHIGNFVEVKNSTLARGAKANHLAYLGDATVGERVNYGAGSITANYDGANKHRTVIGDDVHVGSNCVLVAPVTLGAGATIGGGSTIAKDAPAGQLTVARAKEKTLAGWRRPVKKS",
    "response#3": "MENGVTLPAPETVHFAFDTAIGRDTVIEQNVVFGPGVTIETGAHIRAFSHLEGCHVSRGAVVGPYARLRPGAELAEDTRIGNFVEVKNATIDEGAKVNHLSYIGDAHVGSGSNIGAGTITCNYDGVGKHHTEIGENVFIGSNTMLVAPVTVGNGALTGSGSVITKDVPADALAIARSQPARPVSEIVLAAGQGTRMNSDLPKVLHKVASAPLMVHALKSGASLAPERQIVVAGHGAAEVTKAAQDWDEEIKVVLQEEQLGTAHAVAQARPALDGFKGDAIILYGDTPFIRPETLEAMVEARRTHDVVVLGFDARDPGRYGRLVTEGDRLERIVEFKEASDQERAVTLCNSGVICADADLLWALLDRVGNANSKGEYYLTDIVEIARAEGRRAVAVRCDEEETLGVNSRSLAEAEQVMQTRLRKAALEAGVTMIAPETVYLAADTTFGSDVTIEPFVVIGPGVSIADGAVIHSFSHIVQATIGKNASVGPYARLRPGTSLGDGARIGNFVETKAAILEAGVKVNHLSYIGDAYVGANANIGAGTITCNYDGFNKNKTEIGAGAFIGTNSSLVAPVKIGDGAYIGSGSVITRDVPDDAMALERNQQEVKPGWADRFHAAMRAKRAEKAE"
  },
  {
    "reference": "MAVKYIFVTGGVVSGLGKGITAASLGRLLKARGYKVTMQKFDPYINIDPGTMNPVQHGEVFVTEDGTETDLDLGHYERFIDENLDKNSNVTTGKIYWSVLQKERRGDYGGRTVQVIPHITNEIKSKFYRNFTDEETHIAIIEVGGTVGDIESQPFLEAIRQFQHEVGHENAMLILVSLIPYLRVSQEIKTKPTQSAVKTMQGMGLQPDVIVCRSEVSLDQDTKDKIALFCNVPSNHVLQNLDLEYLYEAPLAMEKEHLAHVACECLNLDCPEPDLTDWKAMVDTLYSLSHEVKIALVGKYTQLHDAYISVVEALKHGGISNQTAVDIKWVDSEEVTEDNVASILSDVDGIIVPGGFGDRGIEGMIHSIKFARENNIPFLGLCLGMQLAIIEFARDVIGYNDAHSIEFEPNTTHPMIALLPDQNGVEDIGGTLRLGSYPCVLDKDSMAYKLFGSENITERHRHRYEVNNDYRSVLVSNGMKLCGLSPDGRIVEMIELPENDFHIATQAHPELKSRPNRPHPLFSGFIKASLEHEKKNS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be optimized for CTP synthase activity, glutaminase activity activity in order to improve glutamine metabolic process, 'de novo' CTP biosynthetic process.2. The protein should have a high affinity for Mg(2+), ATP, UTP, L-glutamine.3. The designed protein have Glutamine amidotransferase domains with specific sequence motifs that are crucial for its activity.4. The protein design should take into account the physiological conditions of the CTP biosynthesis via de novo pathway, CTP from UDP: step 2/2, Pyrimidine metabolism pathway.5. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.6. The protein with the reaction H2O + L-glutamine = L-glutamate + NH4(+), the Nucleophile, Nucleophile; for glutamine hydrolysis must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MAKYIFVTGGVTSSLGKGIIAASLANLLQARGFSVTIQKFDPYINIDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNTPTSQSNNITTGRIYNNVITKERQGAFLGKTVQVVPHITDEIKRNFYQLGETGKYDIVITEIGGCVGDIESLPFIEAVRQAVWELGHNSALVIHLTLVPYLKSAGELKTKPTQHSVRDLSQEGVLPDILVCRTEHPLPPELRKKIALFCNVQINSVIEAMDAETIYDVPLLMKREKLDERVMSKLKLKDAYISVYEAMEHAGYAHKFKVNVKRIQAEEVSAKNAETLFADVDAILVPGGFGDRGNHGKIEAIRFARENKIPFFGICLGLQLASLEFARNVLKLEDANSREFDEDAQNLIIDFMEGQSNNIDMGGTQRLGAYACEIIDNTKAKSAYKSNLVSERHRHRYEFNNQYISDFKAAGMVFSGTNPQTGLVEIIELADHPWFVAVQFHPEFSSRPLKPQPLFYDFIRSKLEDIR",
    "response#2": "RYVFVTGGVVSSLGKGLASAALGALLQARGYSVRLRKLDPYLNVDPGTMSPFEHGEVFVTDDGAETDLDLGHYERFTGVAARNTDSVSSGRIYSNVLEKERRGDYLGKTIQVIPHVTNEIKDFLHVGEDEVDFMLCEIGGTVGDIEGLPFFEAIRQFAQDKPRGECIFMHLTLLPWMAASGELKTKPTQHSVKELRSIGLQPDVLVCRSEVEIPEKEREKIALFCNVRKEDVIAAPDLKSIYEAPLAYHEQGLDQAVLDAFQLQDAYKSIHEALIHGGVHLHTQVQIRWIDADTLIPKTYKSMLSELDGILVPGGFGGRGVDGKMLAIRYARENNVPFFGICFGLQLSLIEIARQVAGLKEAGSTELENPKTPVVCLMGEWEKEGALEARTEQSDKGGTMRLGNYPCTVLDRTKAYEAYQGNMIQERHRHRYEVNIKYKADLEKAGVVFSGMSPDGKLPEIIELKDHAWFVAVQFHPELKSRPFSPHPLFVSFLEDAYKSIAEALTHGGMANRTRVKAEWIDAEIFEREDPAPYLEGFHAILVPGGFGERGTEGKIKAAEFARTRKIPYLGICLGMQMAVIEAARNVAGMDTAGSEEFDHEAGKKRFEPVVYHLKEWVQGNHVARRKVDDDKGGTMRLGAYTANLTQGSRVAQVYGTTTIEERHRHRYEVDTKYREQLEKVGLCFSGMSPDGRLPEIVEWKDHPWFIGVQFHPELKSKPFAPHPLFEGFVDAAQDRQRDADKSLCEALTHGGIANDTRVNIEWIEAESFEKEPHTVAERLKDKKAILVPGGYGERGTAGMISAIQYAREHQIPYMGICFGMQLAVIEVARHLAGIEKANTTEFGVTAEPVVGLLTEWLNGDVKERRSAEDDLGGTMRLGLYPAILAPGSLIQKIYGTSSIQERHRHRYELNIEYREKFEKAGLKFSGLSPDGLLPETVELEGHPWFVGVQFHPELRSRPFDPHPLFTSFI",
    "response#3": "KYLFITGGVCSSLGKGIAASSLGAILKADGYKVNMMKIDPYLNVDAGTMRPYEHGEVYVTGDGTETDLDLGNYERFVRVETSRLSSVTTGQVYQYVIDAERRGEYLGQTVQVIPHITDEIKRRIRVLDENQDSDIVIVEIGGTVGDIEGIPFIEAIRQFSIEEGKDNVKFAHLTLIPSIRGSNELKTKPTQHSVKTLMSMGIQPDFLLCRAQDMLPPHHRQKIALFCNIEEPCIFSGVDVKKSIYEVILSFQEQGADKIVLDKFRLKTKPHDLGPWRSFLKRLKNPKYEVKIGLVGKYVELKDAYKSIVESFVHAGAMNECKVKISWTKAEDLADGDLDLLKGLDGILVAPGFGERGFEGKINAIRYARENKIPFFGICLGMQCAVVEFARNVLQLKEATSIEMNPNTSEPVINMMEDQKRITQMGGTMRLGAYPCHIKKGTKAFSVYGKTEISERHRHRFEFNNDYLKRYESAGMIASGINPASGLVEIMELKDHPFFIGTQYHPELKSTVLNPHPLFVKFVKAVLKNKKG"
  },
  {
    "reference": "MAPNAPFARSLRLQRLHHHDPDRLFIVPLDHSITDGPLSRAHRLDPLVGELASHHVDGIVLHKGSLRHVDPEWFTRTSLIVHLSASTVHAPDPNAKYLVSSVEESLRMGADAVSVHVNLGSEGERHQIADMAAVAEACDRWNVPLLAMMYPRGPKIDDPRDPALVAHAVQVAVDLGADLVKTLYVGSVAAMAEITAASPVPVVVVGGPRDSDESRILAYVDDALRGGAAGVAMGRNVFQAPDPGAMADKLSDLIHNSGTRGAARAPAGAAAGAA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The desired protein should be able to perform 2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate =   dihydroxyacetone phosphate + L-aspartate 4-semialdehyde2. For general function, the protein need meet that Catalyzes aldol condensation between L-aspartate-4- semialdehyde (ASA) and dihydroxyacetone phosphate (DHAP), to form 2- amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate.3. The protein must exhibit fructose-bisphosphate aldolase activity. The designed protein sequence is ",
    "response#1": "LKPIHDRYEIKDIVVSTYQAVSGSGRKGMMELSNQIDSFVNGKEINNNVYPTQIAFNVIPFVDSYHDNNYTKEEMKMIWETKKILSKKINVNPTAVRVPVMIGHSESITIETKKEINIEDVIQDYINCEGLTLMDDVDNIVFPTARSEGSGTDNVYVGRIRKSLSNNNVLNIWVVADNVRG",
    "response#2": "LKPIHDRYEIKDIVVSTYQAVSGSGRKGMMELSNQIDSFVNGKEINNNVYPTQIAFNVIPFVDSYHDNNYTKEEMKMIWETKKILSKKINVNPTAVRVPVMIGHSESITIETKKEINIEDVIQDYINCEGLTLMDDVDNIVFPTARSEGSGTDNVYVGRIRKSLSNNNVLNIWVVADNVREAVQL",
    "response#3": "LKPIHDRYEIKDIVVSTYQAVSGSGRKGMMELSNQIDSFVNGKEINNNVYPTQIAFNVIPFVDSYHDNNYTKEEMKMIWETKKILSKKINVNPTAVRVPVMIGHSESITIETKKEINIEDVIQDYINCEGLTLMDDVDNIVFPTARSEGSGTDNVYVGRIRKSLSNNNVLNIWVVADNVRPADIL"
  },
  {
    "reference": "MDAESALSRGCIEAIFNDPDQAANRFPVPVMQCLQIKQMAPSGQGGDRYRLVMSDGVNYVQTMLATQANHVIHDNKLERGSLTRVKQYTPNNLKGKNILVILDLEVIESFGAQEKIGDPVVLDPRPAETTIAGNDFYGSKKEEMDTKPQMQQLPSNPISATHAGANIYPIEALSPFSSKWTIKARVTFKSDIKTWHKPTGEGKLFSVNLLDESGEIKATGFNDQCDAFYDLLQEGSVYYISSPCRVSLAKKQFSNLPNDYELAFERETVIEKAEDQTNVPQLRFNFCTIQELQSVEKDSTVDVIGVLKEVAEVSEITSKKDGRPFQKRELTLVDDTGYSVRVTIWGKTASAFDANPESVVAFKGTKVSDFGGKSLSLLSSGTMTVDPDIPDAYRLKGWYDSAGRTNTFATHQNLAGVAGATGRKEEIKTISQVKDENLGVDDQAYYTIKATIVFVKQDNFCYPACSNQGCNKKVTAMPDGTWQCEKCNVSHDKPDYRYIMQLNVADHTSHQWLSCFDDTGRIVVGMSANELMELKENDDAKFMAAFEAVNCKKLVFRCRAKMDNFGDTQRVRYQVFSATLVDYKTEGNKLVELIKQFSIN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be optimized for double-stranded DNA binding activity in order to improve premeiotic DNA replication.2. The designed protein must have at least one OB, Replication factor A C-terminal domain.3. The protein must exhibit the following characteristics: As part of the replication protein A (RPA/RP-A), a single- stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. The designed protein sequence is ",
    "response#1": "WTIRAKVISKNDIKTWNNARGSGKLFSTVFMDNSSEIRATAFNDACDKFYDLLQEDQVYYISGANIKVANKQFNNVKNEYEMN",
    "response#2": "YIAGMPGTGKTATVKEIIKELQAKKKQQGGGGGLNFQFIEINGMQLSDPHQLYHILYNKMQKTRKSLEPKKISSQDALRLIQRNFELKNKKKQFRVILVDEFDSLITKKQTVIYNLFEWPNKPNSKLIIIAIANTMNLPDTLLPRVKSRI",
    "response#3": "SGGCLYIAGMPGTGKTATVKEIIKELQAKKKQQGGGGGLNFQFIEINGMQLSDPHQLYHILYNKMQKTRKSLEPKKISSQDALRLIQRNFELKNKKKQFRVILVDEFDSLITKKQTVIYNLFEWPNKPNSKLIIIAIANTMNLPDTLLPRVKSRMGLQKVC"
  },
  {
    "reference": "MKVAVIGISGAVGQEFLRVLHERNFPMDELVLFGSARSAGRTYEFKGKQLTVKELKHGDDFKDIDIALTSAGASVSKEYAETITKHGAIMIDNSSAFRYDNDVPLVVPEVNPEDARNRPRNIIANPNCSTIQMVVALKPINNLSPIRRLHVATYQAASGAGAQGIAELEQQVKEIADGKEVTISKFQYQLAMNIIPHIDVFLENDYTKEEMKMNWETKKIMHTDAEVSATCVRIPVARAHSEAIWVETEEPLSAEEVKTAFNNFEGLTVIDNPSEKQYPMPLFVSGKNDVYVGRIRKDISNPNGITFWCVGDQIKKGAALNAVQIAEWLIKNGEVK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be optimized for NAD binding activity in order to improve diaminopimelate biosynthetic process.2. The protein should be able to bind phosphate, substrate ligand in a variety of conditions3. The protein design should consider the endogenous proteins and enzymes present in the Amino-acid biosynthesis pathway to avoid interference with native metabolic processes.4. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "LQEKFGLKRVAYTTYQAVSGSGIKGKRDLAEGANGKAPEAYPHPIYNNVLPHIDIFLEDGYTKEEQKMIDETRKILNEPDLRVTATCARVPVQDSHSVEIDVTLIEEATTEEIKALFDKDPRVVLVDNPANNEYPLAINSTGKDEVFVGRIRRDDSLDNTFHVWCTSDNLLF",
    "response#2": "LKPLHDAAVIKRVVVASYQSTSGAGKRAMDELFNQTKGIYVNDPIRKEQFTKQIAFNVIPHIDVFLDGGITKEEWKMMVETKKILDPSIRVSATCVRVATFIGHGEAINVEFERSISASEARDLLREAPGISVIDNPAAESYITPVECVGEDAVFVSRIRDDHTVENGLSMWVVADNLRR",
    "response#3": "LKPLHDAAVIKRVVVASYQSTSGAGKRAMDELFNQTKGIYVNDPIRKEQFTKQIAFNVIPHIDVFLDGGITKEEWKMMVETKKILDPSIRVSATCVRVATFIGHGEAINVEFERSISASEARDLLREAPGISVIDNPAAESYITPVECVGEDAVFVSRIRDDHTVENGLSMWVVADNLRR"
  },
  {
    "reference": "MVNNVVSIEKMKALWHSEVHDEQKWAVNMKLLRALGMFAGGVVLMRSYGDLMGV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: A component of the complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins..2. The protein should have a Helical; Signal-anchor domain that is long enough to span the entire membrane.3. The designed protein localizes to the mitochondrion, mitochondrial outer membrane translocase complex. The designed protein sequence is ",
    "response#1": "LTLVVTLDDFLIHSNWDTQHGWRTGKRPGLDYFLGYLSQYYEIVVFSSNSQIYSDKTVNKLDPYHAYISYALFREACRYKDGKLIKDLSLLNRDLGKTVMIDVDEDSAALQPENSIIVKKWEGQPDEYLISLIPFLEYLATQPVKDVL",
    "response#2": "LTLVVTLDDFLIHSNWDTQHGWRTGKRPGLDYFLGYLSQYYEIVVFSSNSQIYSDKTVNKLDPYHAYISYALFREACRYKDGKLIKDLSLLNRDLGKTVMIDVDEDSAALQPENSIIVKKWEGQPDEYLISLIPFLEYLATQPVKDVL",
    "response#3": "QYRRPLTLVVTLDDFLIHSNWDTQHGWRTGKRPGLDYFLGYLSQYYEIVVFSSNSQIYSDKTVNKLDPYHAYISYALFREACRYKDGKLIKDLSLLNRDLGKTVMIDVDEDSAALQPENSIIVKKWEGQPDEYLISLIPFLEYR"
  },
  {
    "reference": "MGKIIGIDLGTTNSCVAILEGNTPKVIENSEGARTTPSIIAYMEDGEILVGAPAKRQAVTNPRNTLYAVKRLIGRKFEEKEVQKDIGLMPYSIVKADNGDAWVSVRDQKLAPPQVSAEVLRKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRIAGLDVKRIINEPTAAALAFGLDKNEKGDRKIAVYDLGGGTFDISIIEIADVDGEKQFEVLSTNGDTFLGGEDFDQRIIDYIIGEFKKDQGVDLSKDVLALQRLKEAAEKAKIELSSSQQTEINLPYITADASGPKHLNLKMTRAKLESLVEELITRTIEPCRTAIKDAGVKVSDIDDVILVGGMTRMPKVQEQVKEFFGKEARKDVNPDEAVAVGAAIQGSVLSGDRKDVLLLDVTPLSLGIETLGGVMTKMITKNTTIPTKHAQVFSTADDNQPAVTIKVYQGEREMATGNKLLGEFNLEGIPPAPRGTPQIEVSFDIDANGILHVGAKDKATGKENRITIKANSGLSEDEIQRMVKDAEANAEEDKKARELADARNQADALIHSTRKAVTEYGDKLEAGEKEKIEAAIKELEDAARGGDKTEIDAKVNALSEASQKLGEKVYADMQAKAGEQGAAGAAGAGAQQQAQPQDDNVVDAEFKEVNDKK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Acts as a chaperone.. The designed protein sequence is ",
    "response#1": "SDIQQGFLAKELQAEALCRLDRKIKATIEQFMKILEEIDTMVLPENFKDSRLKRKNLVKKVQVFLAECDG",
    "response#2": "SDIQQGFLAKELQAEALCRLDRKIKATIEQFMKILEEIDTMVLPENFKDSRLKRKNLVKKVQVFLAECDC",
    "response#3": "KQLLELIVHSLYTHPEIFLRELISNASDALSKVRFNALTDESIINSDAGLAIRITLDPEAHTIVIEDNGTGMTEEELILNLGTVARSGTLGFLQALKEQQKELDGNLIGQFGVGFYSVFMVTDEVTVETRSSGADSAGYRWRSGAAGTFTIERIEKEQRGTKISFALKDEFKEFSEAYRIEQIIRKYSNFK"
  },
  {
    "reference": "MSEIKEARLQKASSLVSKGFASYAQSFRVSHTTSFLIQKFDYLENGQEENFSVSLAGRVMAKRVMGKIAFFTISDQDGQIQLYLEKRIINLNLEKQKLLSFEDIKEIVDIGDWIGVYGTIKKTNKGELSIKVEKWEMLSKSLQPLPDKWHGLTDIEKRYRQRYLDLIVNPHSKNVFKTRAKCISFIRKWLDNRNFLEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMVVGGFEKVYELGRIFRNEGISTRHNPEFTSVEIYEAYSDYVDMMNLTEELIKDILADACGSLTINYQNKEIDFSKPWLRISMKDIVKKYTGIDFHSFNGDFLAAKKAVKNINVDFSNKVNTIGRLLNEVFEQKVESKLIEPTFVIDYPVEISPLARPHFDNKEIVQRFELFIVGRELANAFSELIDPVDQRERMQLQQSLRDKGDLEAHCIDEDFLNALEIGMPPTGGLGIGIDRLIMLITNSASIRDVIPFPLLKPEISSKKSEKLPLNEVK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The desired protein should be able to perform ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-   tRNA(Lys)2. The protein requires Mg(2+) to activate a critical catalytic site.3. The designed protein must possess ATP binding to support lysyl-tRNA aminoacylation under certain conditions. The designed protein sequence is ",
    "response#1": "PDVFFWYTRLSGVLSILAFVSSDLIQRLGGIWALRWIYYLGFTASLPYGVLLILLSMGVRRRKKAAWRILMLLFGVYFGFALLVVGKLALEPDRSVALGELFTLAAYAAVLTLLAAARSEFNALPDRANRRLALAVFSGVLAVSGLLGTGLVTLTNRDPQGDFVTDALYAALQTVGGPGVTGEPIDVDVPTRVNLLLGVLGTGLLIITFWALL",
    "response#2": "GLLERYGQDDSLGYFATRRDKAVIFAPNGRAAITYRVEVGVCLVSADPIGDKAAWGQAISAWLQVCQAYGWAPAVMGASAEGALAYREHGLSALELGDEAILYPRSFSISGPHMAAVRQAVNRARRSGLSVRIRRHRELGAEETKQVIANADLWRDGNTERGFSMALGRLGDSTDGDCLLVEAVDPDGNAVAMLSLVPWGSNGVSLELMRRSRQSPNGTIEFMVTELLTRAEGMGIARVSLNFVMFRSVYADGSRIGAGPVLRLWRSVLMFASRFWQFEQLYRSNVKYQPEWVPRYSCRRLLAEYGEQDSLGYFALRRDKDVIVAPNGKAAISYRVEGAVSLASGDPLGDPESWDQAIELWLAECRAHAWIPGVVSAGERAAHVYRRHGFDALELGDEAILELTDFSLEGREMRQVRQAVRRVERAGYRVRIRRHAQIPAEEMSRLIASADRWREGATERGFSMALGRLGDPTDGRCVMVEAFDARGELRGLLSFVPWGRHGLSLDLMRRDRTAENGLNEYMVAKVAEQAALIGAQRMSLNFAMLRAAFERGSQLGAGPVARGWYRFLSLASKFWQLESLYLANAKYNPIWAPRFVCP",
    "response#3": "PDVFFWYTRLSGVLSILAFVSSDLIQRLGGIWALRWIYYLGFTASLPYGVLLILLSMGVRRRKKAAWRILMLLFGVYFGFALLVVGKLALEPDRSVALGELFTLAAYAAVLTLLAAARSEFNALPDRANRRLALAVFSGVLAVSGLLGTGLVTLTNRDPQGDFVTDALYAALQTVGGPGVTGEPIDVDVPTRVNLLLGVLGTGLLIITFWALS"
  },
  {
    "reference": "MTEVISKTSLFLGACGNHHRVDDFSFSPVSFGGFGLKKSFSCLKLKSQKPLRSVFYGKQIVFGDSQDESFRRSSAITAQTTLRIGTAQKWWEKGLKDNMREISSAQELVDSLTNAGDKLVVVDFFSPGCGGCKALHPKICQFAEMNPDVQFLQVNYEEHKSMCYSLGVHVLPFFRFYRGSQGRVCSFSCTNATIKKFRDALAKHGPDRCSLGPTKGLEEKELVALAANKELNFTYTPKPVPVEKEAATPDSNPSLPVPLPSMSSNDEKTLVSAGR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Thiol-disulfide oxidoreductase that may participate in various redox reactions; Possesses insulin disulfide bonds reducing activity.2. The protein should be optimized for chloroplast stroma, chloroplast membrane, chloroplast-specific folding and stability, while maintaining oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor.3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "LLFFFLAGLTLTFTPCVLPMLPILSGVVLRGRPGGGRGFVLSLAYVLPMALCFALLGALMGMFGASLNLQAQLQSPWVLVPFAAFFALFAVAMFGFFELRLPGFIREPLDRLAGDARGGSILGAATLGVLSSLLVSPCVSAPLAASLLYISASGDAWGGGLQLFALGLGMGTPLVVFGAGGGALLPKSGAWMNGVRNAFGVLLLAVAVWLLERVL",
    "response#2": "LLFFFLAGLTLTFTPCVLPMLPILSGVVLRGRPGGGRGFVLSLAYVLPMALCFALLGALMGMFGASLNLQAQLQSPWVLVPFAAFFALFAVAMFGFFELRLPGFIREPLDRLAGDARGGSILGAATLGVLSSLLVSPCVSAPLAASLLYISASGDAWGGGLQLFALGLGMGTPLVVFGAGGGALLPKSGAWMNGVRNAFGVLLLAVAVWLLERVL",
    "response#3": "LLFFFLAGLTLTFTPCVLPMLPILSGVVLRGRPGGGRGFVLSLAYVLPMALCFALLGALMGMFGASLNLQAQLQSPWVLVPFAAFFALFAVAMFGFFELRLPGFIREPLDRLAGDARGGSILGAATLGVLSSLLVSPCVSAPLAASLLYISASGDAWGGGLQLFALGLGMGTPLVVFGAGGGALLPKSGAWMNGVRNAFGVLLLAVAVWLLERVL"
  },
  {
    "reference": "MIDSAHTNSSSSSASSHEDMTDFGFKHVPREEKKPLVRQVFESVAGKYDIMNDAMSLGIHRIWKRIFITELAPRSNLKLLDLAGGTGDITFGWLKRGGGHAILSDINPAMLEVGRERAIKEGLIDRLDVKEIDAESLPFPDASFDRVSIAFGLRNCTDKDAVLREVRRVLKPGGRFCCLEFSKVAVAALSPVYDLWSFQILPKMGKMIAGDSESYQYLAESIRMFPDQDRLADMMREAGLENVKYQNLSGGIVAIHSGWRL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be optimized for activity in menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2, menaquinone biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The protein design should emphasize Polar residues compositional bias.3. The protein design must be able to perform demethylmenaquinone methyltransferase activity, S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity in order to contribute to ubiquinone biosynthetic process, menaquinone biosynthetic process, aerobic respiration, methylation. The designed protein sequence is ",
    "response#1": "MVILQRIKNAKVTINGQELESINGEGLLLFSCHKR",
    "response#2": "MSGKPTVFITGCDTGFGKLTAKRLDSLGWHVVAACMTPGKAEELRA",
    "response#3": "MFGIDWTEIAKHAGSYDFLNRLLSLRQDIVVQ"
  },
  {
    "reference": "MSWKGFTKALARTPQTLRSKFNVGEITKDPIYEDAGRRFKSLETEAKKLAEDAKKYTDAINGLLNHQIGFADACIEIYKPISGRASDPESYEQEGNAEGIEAAEAYKEIVYDLQKNLASEMDVINTRIVNPTGELLKIVKDVDKLLLKRDHKQLDYDRHRSSFKKLQEKKDKSLKDEKKLYEAETAFEQSSQEYEYYNEMLKEELPKLFALAQSFIAPLFQGFYYMQLNVYYVLYEKMSHCEIQYFDFNTDILESYERRRGDVKDRAEALTITKFKTAKPTYKRPGMGPGGKDATASSSSSFSSKREEAAAEPSSSTATDIPPPYSTPSVAGASDYSTPSAGYQTVQTTTTTTEAAAAQYPQAAFPPPPVMPQPAAAAVTTPVAAPVAAAAAAVPVPPPAPAPAAAPAAEHVVALYDYAAQAAGDLSFHAGDRIEVVSRTDNQNEWWIGRLNGAQGQFPGNYVQLE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that can perform lipid binding in Rvs161p-Rvs167p complex, mating projection tip, medial cortex to support cellular response to DNA damage stimulus. The designed protein sequence is ",
    "response#1": "VRPPRCFSHDNVLRPFDEVESEGWFLLKTAHKGKHASDSYIWHHIINQECTIIISDHRIILSKSKKNFLHSSFLFQIPFKFIKSNEMVDDGVLLEFDPPQNLGLLDRDVKSKVIPE",
    "response#2": "VMLKTGHIEQTIDKEYEFQEKRYRTMEENSIKLQKNLRLYLDSLRLLTNSQINIAESLNSFYGTNNDKRSSNISRTGSVNTGDKIQTIHEEEGEEKEEEENDNTTTTTTTTTTTITTTNTTYNYNNLIQEYYATIKQLNDSCIANLENPYNQTVLNPIARFNSYYIEINEIIKKRHNKLLDYDAMKNKLRKLIENPTTNISPSMKTNIIELNHNQYEEKLKIYNQELTEVESKYVEINNQLLIELPKLINHRISYFDPSFESFVKIQLRFFNENYHVLNQLQLKLDAQTRQDYL",
    "response#3": "TRERKLNNKTSPTTPSSSGVSTGTTFDIDIDNFDTVSSSSSSSLLSSSSLLISPPGGEQSSLVYFQFESRNDNNSQMLKINLSSIRFIFLKRFTEELRLFLNNVNMMREYLKSSIYSAATVISQNRSTLFYEIEIQNPYIVIPLSSLSNKIFIVDLGKINIKNQFEKIIPTSTGEIDMILMEPVIIENIIIEAQNIKLLSGLNSEIKKSENNNNNNNNSNTNQRSYGTMVSDVNVSINLKTPLFSELNLKQYLEQCMKLQSWEKHFTISQFELNVSEYELKSLIELLDGNLSEFSSEILNDANSSFDQQKQQEIEKLKLQQEIEDNNNDNEDEQVNKLLQLIKEQVGYTYCKLGKFSIMFMKRDGTDIDDRLVLFYVSEAKMDIIKEEMETSMNIEIKSVQMKDFSKETHPHLRNILTPLSLKSVNEKRQQKEQILPQIIIKGSIKPPPIQQSLFQISIDGMFMIFVPHSWIPVQDSIMKLSKFATEAWNRYTIKVYGEAPTNDEIIQSGLSLFSLSIKDIKISIPGDCTVNSGQGENEYSLFIRSSLDIQTTSRGAMGVETITLIDANNIQIYRNQLVDTNNENINNNNNNNLTTSTTTTTTTNKNQSNIKIIAPFKIVFQTTSTLLNSESSLSINNIVVTFSYQDFKLMMKIINSIIESNNQNLIQEKMKRNYLKKYRDPNECTSDDELEEQLQQLENDDYNDDNYNSNGNNNNNNSNNQLPVKEDSQLQKYLSISLEKGEFILNDDHKISSPIKLLSIGVDGLKSNIFSFPQKNQIALSLDANMKAGYFNKNIGIWEPLIENWGFSFTSNNSIEGGWMVNFNSKIPLYINITKIFIDTSISTYQIWADDYYSQQKKDKKNKSNDYDNDEIIEDTVEQVNNDLIKPADDQKNNNNNKNDNNNNNNNNNNNNNNNNNNNNNNN"
  },
  {
    "reference": "MVDTNGVHFIIDAFSCDDQILNDANLLENILKKAVSDLGMVMLSSRFHTFSPQGVTGVIVLSTSHISIHTWPEHRYASIDLYTCGNPNWPVLNELLLTLKAEKAHVYEISRGNEHISSPAWKEISLTANSTQDDIKKKVIEIKEKQGNDWDKLQLKEILEDKHQIYHSESTPFQDILLLEVNDLRLYLNQELQFSSLDERHYHEALVYPVMENAKKHERVLILGGGDGLALREVLKYPDVLHVDLVDIDRKIIELAKTNPKLLVLNMGSFHDERVSVHTEDAKKFIDRKQEPYDVIFIDFPDPADPIISSLYTKEVFKQITNLLEQDGVLVCQSGSPEDTPTVYWSIAKTLESAGLFTRGYVSVVPSFGLWGFHLASRLDLRSIKFNISVPHRALKDDTSYLFKLPDKLVSMQKNAFINSKDSLELHNHYLRELKIMY",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein with adenosylmethionine decarboxylase activity, spermidine synthase activity is needed to enhance spermidine biosynthetic process in a specific context.2. The presence of pyruvate is necessary for the protein to function in the desired environment.3. The protein must exhibit the following characteristics: Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine..4. The protein's Proton acceptor, Proton acceptor; for processing activity, Schiff-base intermediate with substrate; via pyruvic acid, Proton donor; for catalytic activity must be carefully engineered to ensure that the amino acids involved in putrescine + S-adenosyl 3-(methylsulfanyl)propylamine = H(+) +   S-methyl-5'-thioadenosine + spermidine are properly positioned for optimal catalysis.5. We require a protein that can specifically bind to the metabolites produced in the spermidine biosynthesis, spermidine from putrescine: step 1/1 pathway.6. The spermidine, S-methyl-5'-thioadenosine binding site should be located in a region of the protein that is accessible to the ligand.7. The protein contains novel PABS domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MRGRVVEPVSPGLTRTWEIGRVHHRETTPFQHLLIADTAHGVTLFCDDERQSAESTQLTYHEALYVPAVLWARRRERVLVVGSSEGVVSELAIACGATLVDHVDVDTAAVRACAAHLPYGYTPDALVAAERGDGPVRVHYADGREWVRGEGPRYDVIVVDLPDERPDDAGNQLNRLYTRDFLADCRDRLTDGGVVVSQAGSAAVWRDATLRAAWDRFHDVFAHVRYVGSDEHEWSFLVGGGAGDSSASGIDPAAAEEPVGPGLTRVWELSEVLVDTRTEYQHLVIGRTEQGISLFSDNDRQSTEFSQLTYHEAMVVPALVLAEKLDDVLIIGSGEGVASQLAIAAGATRVDHVDIDRTAVHLCAEHLPYGYTNAELNAAAAGEGPIQVFFADGWEFLADAAAAGNRYDVIVIDLPDERVEDAQHNRLYEADFLRRCRDVLAPGGVLTAQAGCATMWRNETLKRSWQRFHEQFGTVVHYGSDEHEWSFLFGLTDRIDDPVAGMVDEA",
    "response#2": "MNELWYTEEHTDKVRFSIKVDEQLYSGKSDFQRIDVFKSQEFGTFFTLDGLMMVTEKDEFIYHDMIVHVPMATNPNIKNVLVIGAGDGGTVRELTRYETIEKIDMVEIDKLVVDVCREYLPQTANKLDDPRVSLFFEDGLKFVRSVENKYDLIIVDSTDPFGPGEGLFTREFYGNCFKALKEDGILVNQHESPYYEEYARGMKRAHKRIKECFPVCRVYQAHIPTYPSGHWLFGFASEEPVGPGLTRVWELSEVLVDTRTEYQHLVIGRTEQGISLFSDNDRQSTEFSQLTYHEAMVVPALVLAEKLDDVLIIGSGEGVASQLAIAAGATRVDHVDIDRTAVHLCAEHLPYGYTNAELNAAAAGEGPIQVFFADGWEFLADAAAAGNRYDVIVIDLPDERVEDAQHNRLYEADFLRRCRDVLAPGGVLTAQAGCATMWRNETLKRSWQRFHEQFGTVVHYGSDEHEWSFLFGLTDRIDDPVAGMVDL",
    "response#3": "RGRVVEPVSPGLTRTWEIGRVHHRETTPFQHLLIADTAHGVTLFCDDERQSAESTQLTYHEALYVPAVLWARRRERVLVVGSSEGVVSELAIACGATLVDHVDVDTAAVRACAAHLPYGYTPDALVAAERGDGPVRVHYADGREWVRGEGPRYDVIVVDLPDERPDDAGNQLNRLYTRDFLADCRDRLTDGGVVVSQAGSAAVWRDATLRAAWDRFHDVFAHVRYVGSDEHEWSFLVGGVEEPVGPGLTRVWELSEVLVDTRTEYQHLVIGRTEQGISLFSDNDRQSTEFSQLTYHEAMVVPALVLAEKLDDVLIIGSGEGVASQLAIAAGATRVDHVDIDRTAVHLCAEHLPYGYTNAELNAAAAGEGPIQVFFADGWEFLADAAAAGNRYDVIVIDLPDERVEDAQHNRLYEADFLRRCRDVLAPGGVLTAQAGCATMWRNETLKRSWQRFHEQFGTVVHYGSDEHEWSFLFGLTDRIDDPVAGMVDGFPPDALVAAPAPEEPVGPGLTRVWELSEVLVDTRTEYQHLVIGRTEQGISLFSDNDRQSTEFSQLTYHEAMVVPALVLAEKLDDVLIIGSGEGVASQLAIAAGATRVDHVDIDRTAVHLCAEHLPYGYTNAELNAAAAGEGPIQVFFADGWEFLADAAAAGNRYDVIVIDLPDERVEDAQHNRLYEADFLRRCRDVLAPGGVLTAQAGCATMWRNETLKRSWQRFHEQFGTVVHYGSDEHEWSFLFGLTDRIDDPVAGMVD"
  },
  {
    "reference": "MRRTRAEEREKEAKKRKLLNELGESCLPYLTPKDAGFYQLWKTKYSKLILKNSEKIPQDLHEEVQKAFFALRKHVCFFQDLVRIKGRDFVTPVSRILIGNPGCTYKYLNTRLFTVPWPTEGYDINYSNPEISLACNALSRLNDYLQRETLLALQEQDLAETTRMQDLPFGDGNQDDASNITELEAVLQDQNPSPVPKIDSTILKERTSYNLTLLNYMDPQKMPSLKLEPYFGMGRMAVSWHHDENLMERSTVAVYSYSREEVDGGVLEEKSAAGRDPDVWHVGLKVAWDIETPGLAIPLHQGDCYLMLDNLNTTHQHCVLAGRQSRFSSTHRVAECSTGTLPYILDKCKAALKNLNTDADLKVPSLKSLEVGDITRVEKTHNEVEFEWLRQFWFQGKRYRRCTDWWDQPMADLEVLWRQMELMTSLLLHELRKEEQMQEQRNEKTSSLLPLLVERHALRQEWLMPFTTYRKLTGGRETEMQALLGGQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be involved in RNA repair, DNA dealkylation involved in DNA repair, oxidative single-stranded RNA demethylation by localizing to nuclear speck, cytoplasm and exhibiting oxidative RNA demethylase activity, oxidative DNA demethylase activity, metal ion binding.2. The protein should have a fast turnover rate for 2-oxoglutarate + an N(6)-methyladenosine in mRNA + O2 = an   adenosine in mRNA + CO2 + formaldehyde + succinate.3. The presence of Fe(2+) is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "GTLEYIFGKCNIALQNLQTDSNSAALSLKSLDVAILKQVEEIHNEVEFEWLRQFWFQGKRYRKFTDWWFEPIAKLEEFWKKMELMTSLMLCEVRKEKQAVEQRNNIVSCLLPHLSEREELRKEWTTC",
    "response#2": "GTLPYILGRCNAALENLHGDTALGCLALKSLDIGTIKGVEEIHNEVEFEWLRQFWFQGKRYQKCTDWWDQPMAVLEEHWRKMELMTSHLLCELRKEGQVEEQRKERIECLLPLLEERHELRQEWFVRCHSPLALSLPEEEKPRCHPYWQMGDPHLPLPFNLEDIIIKLAR",
    "response#3": "DPLQMLHLKQEPYFGMGKMAVSWHHDENLVKRSTVAVYSYSCEGSVIDTADKQDPEGRDPAVWHVGLKVAWDIKTPGLAIPLHQGDCYFMLDDLNMTHQHCVLAGLQPRFSSTHRVAE"
  },
  {
    "reference": "MAFSSIDEILADVKKGKPVIVVDDGSRENEGDLFVAAEKVSKESIAFMIREGRGLFCVPMDSRRLDELGLEFLGERHSDALASPFTMSVDARKGTTTGMSASDRLLTVKALLEGNAGELKRPGHVFPLRAHELGVFGRRGHTEAAVDLSRLAGLAPAGVICEIINDDGSMARLPDLEKYAKKHGLKIISVDEIAKYRHSLGGVSEKCSARKSDGCSVRRSAQAKLPTRFGEFEAVSFESVDIEHAYLALVKGNVGGRENVLVRIHSECLTGDALFSRRCDCGSQLEKSLELISAKGEGVLIYSRQEGRGIGLFNKIAAYSLQDQGFDTVQANKKLGFTADLRDYRVCAEILKDLGVHSVRLLTNNPLKVAGLEKHGIRVSERVPLQTPSTQENRRYLRTKKQKLGHLLEV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit zinc ion binding, magnesium ion binding.2. A protein that is capable of catalyzing reactions within the 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway with high efficiency and specificity.3. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity.4. The GTP, Mg(2+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "VASCKLPTPFGVFDMHGFQEVGTQKEHIALTLGDIGDGASVLARTHSECLTGDALFSMRCDCGYQLDEALRSIAAEGRGILLYLRQEGRGIGLLNKIRAYNLQDQGADTVEANEQLGFAADMRDYSMCKPMLYHLGISRIRLMTNNPRKVDSLTKLGIEVVERVI",
    "response#2": "QVSKTRLPTKYGEFTCFTYRDTVRDQYHLALVMGDIRQQESVLVRVHSQCLTGEVFKSLRCDCGDQLDMAMQIIAKEGVGVLLYLRQEGRGIGLHNKLRAYALQDKGKDTVEANIALGFPPDKRDYGMGAQILRHLGVKRMRLLTNNPVKLIALQGYGLETVERII",
    "response#3": "REAEITLPTDFGTFHAIGYSNSLDTKEHIALVKGDISTGEPVLVRVHSECLTGDVFGSHRCDCGPQLHAALAQIEREGKGVLLYMRQEGRGIGLLNKLRAYKLQEEGFDTVEANEKLGFPADLRDYGIGAQILKDLGLQSLRLLTNNPRKIAGLQGYDLEVVERVG"
  },
  {
    "reference": "MESRYAIILAAGKGTRMKSKLYKVLHPVCGKPMVEHIMNRVTELSPTEIVTIVGHGADLVKAQLGERSAYALQAEQLGTGHAVLQATPFLQGKKGTTLVISGDTPLLTSETLERLFEYHEGKHASATILTAHAEDPTGYGRIIRDRVGIVEKIVEQKDASVSEAQVQEINTGTYCFDNELLFEALNQVDTNNAQGEYYLTDIIEILKKADHTVAAYQTENFEESIGVNDRVALAEANRIMRQRINTEHMRNGVSFVDPESTYIEAGITIGAETVIEPGVFLKGNTTIGENCFISGQTQIIDSTIGNDVSIYSSVIEESTIRDHVDVGPFAHLRPQAEIHEHAHIGNFVEVKKAVIGEHTKVGHLTYVGDATLGKEINVGCGVVFVNYDGKHKHHTTVGDHAFIGSASNLVGPVSLGDNAVVAAGSTITNDIPENGMGIARTRQENKVDFAKKLPYNQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be optimized for UDP-N-acetylglucosamine diphosphorylase activity. The designed protein sequence is ",
    "response#1": "KKLEEEQNVESLVCVTAQHRQMLDQVLEVFDIKPDFDLNIMKERQNLSDITVNALSGLYDLIGELKPDIVLVQGDTTTTFAGALAAFYHRIPVGHVEAGLRTNDRYSPFPEEINRRLTGVLSTLHFAPTKRNRENLLKENVMGKIYVTGNTVIDALRYTVKENHVFEDPILRNMDFSDGRYILLTSHRRENIGKPLENICRAVRRIVEGFEDVKVIYPVHMNPAVREIVFPMLENVERVFLIDPVNVIDMHNLMARSYLIMTDSGGIQEEAPALGKPVIVLRKETERPEAIEAGVAVLGGVEEERIFELAKKLLVDREEYEKMAKAVNPFGDGRASERIVKAL",
    "response#2": "KKLEEEQNVESLVCVTAQHRQMLDQVLEVFDIKPDFDLNIMKERQNLSDITVNALSGLYDLIGELKPDIVLVQGDTTTTFAGALAAFYHRIPVGHVEAGLRTNDRYSPFPEEINRRLTGVLSTLHFAPTKRNRENLLKENVMGKIYVTGNTVIDALRYTVKENHVFEDPILRNMDFSDGRYILLTSHRRENIGKPLENICRAVRRIVEGFEDVKVIYPVHMNPAVREIVFPMLENVERVFLIDPVNVIDMHNLMARSYLIMTDSGGIQEEAPALGKPVIVLRKETERPEAIEAGVAVLGGVEEERIFELAKKLLVDREEYEKMAKAVNPFGDGRASERIVKALS",
    "response#3": "KKLEEEQNVESLVCVTAQHRQMLDQVLEVFDIKPDFDLNIMKERQNLSDITVNALSGLYDLIGELKPDIVLVQGDTTTTFAGALAAFYHRIPVGHVEAGLRTNDRYSPFPEEINRRLTGVLSTLHFAPTKRNRENLLKENVMGKIYVTGNTVIDALRYTVKENHVFEDPILRNMDFSDGRYILLTSHRRENIGKPLENICRAVRRIVEGFEDVKVIYPVHMNPAVREIVFPMLENVERVFLIDPVNVIDMHNLMARSYLIMTDSGGIQEEAPALGKPVIVLRKETERPEAIEAGVAVLGGVEEERIFELAKKLLVDREEYEKMAKAVNPFGDGRASERIVKAFL"
  },
  {
    "reference": "MTAPIAAIILAAGKGTRMKSDTHKVLHPLAGRPMLLHLIDSAAALDPARTIVVVGAGREQVEAAVHPYGAETVHQAEQRGTGHAVRMAEAALAGFDGDVLILYGDVPLVPTATMKRMLDRLHGDGEPALVVLGFRPAEPGAYGRVIANPDGSLVKIVEFKDASELERAETLCNSGLMAARARDLFALLGQVTDDNAAGEYYLTDVVGLAVSSGRLAAVIEAAADEVEGVNSRGELAALEVSWQQARRARAMADGATLIAPETVWFAWDTRLGRDVLIEPNVVFGPGVDVADRVTIHAFSHIEGAQIASGAVVGPYARLRPGTVLGEDAKVGNFVETKKAVLGKGAKANHLTYLGDAEVGAGANIGAGTITCNYDGFFKYRTNIGEGAFIGSNSALVAPVTIGKDATVAAGSVVTRDVEPGALALGRARQEARPGWGTRFREAMAARKRGEKPE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The acetyl-CoA, Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.2. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc)3. The protein should have a specific function in peptidoglycan biosynthetic process and should be localized to cytoplasm in order to carry out this function efficiently.4. Ideal protein should have a binding affinity for key enzymes in the Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway to enhance the overall efficiency of the pathway.5. The designed protein must have at least one MobA-like NTP transferase domain. The designed protein sequence is ",
    "response#1": "MEGGATLVAPETVWLAFDTAIGRDVTIEPNVFFGPGVVVEDGAHIMANCHLVGARVGKGARVGPFARFRPGADIGTGARVGNFVEVKNARLEKGAKANHLAYIGDGRVGEGANIGAGTIFCNYDGFNKHFTDVGNGAFVGSNSSLVAPVKIGDGAYIGSGSVISRDVAPDALALERAKLRPEVVRKDAKTPDGRYKYLIRIADGHEVEVVYIPVGDVTINRDTTVYPNTYLMGKTRIGEDVTIGPNVLIEGVSMTDPASTYIDAGVSIGKDTILMPNTYLHGETEIGEGNVIGPNTIIRDSKIGNRNKILASVLEGATVEDDVDMGPFARLRKGAHLKSHVHMGNFGEVKDSTLEEGVKMGHFSYIGNAKIGANTNIGAGTITANYDGENKLPTEIGEDAFIGVDTMLVAPLKLGDGARTGAGAIVTKNVAEDTLVVIPRNDMR",
    "response#2": "MSLAIILAAGRGTRMRSRLDKMLHPVAGRPLFDAAIAAASGCEPVQTVLVVAPHNAEQLRAYAAPRYPDLAFVVQTEQLGTGHAPAMALPLLDPTIDDVMILFGDHPLDTPEMVAATLAEHRASDALVTMATEVADPTGYGRILRQPDGSVAGIVEEKDACPAQRAIREINTGTYAFDAALLRDALGKLSTDNDQGEEYLTDVFGILRAAGHPVRAHRVADPVEVEGVNDRVQLATARRVLNDRLLTAAMRAGVALRDPSRLDVRGSLTCGHDVEIDVGCVFEGDVELGEGVRVGPHCVIRDSHIGAETVIEPHSVIEGAVVAGHNRIGPFARLRPGTRLAVGAKVGNFVETKNAEVGEGSKINHLSYVGDARLGRDVNVGAGTITCNYDGANKHRTEIGDEAFIGSNTALVAPVSVGRGATVGAGSTIDRDVADNALAVERSPPRKIPGWVRERRPGSAAAKAAEQAKAGGQQPGGEQ",
    "response#3": "MEGGATLVAPETVWLAFDTAIGRDVTIEPNVFFGPGVVVEDGAHIMANCHLVGARVGKGARVGPFARFRPGADIGTGARVGNFVEVKNARLEKGAKANHLAYIGDGRVGEGANIGAGTIFCNYDGFNKHFTDVGNGAFVGSNSSLVAPVKIGDGAYIGSGSVISRDVAPDALALERAKIKVVPPEITSDGECGHDVEIDVGCVFEGDVELGEGVRVGPHCVIRNIYIDADVTINRDTTVYPNTYLMGKTRIGEDVTIGPNVLIRDSVIHDNVSIEGFVVMEGAEVETGARVGPFSRIRPSTVLRKGVEVGNFVEVKNSRLDENTKANHLTYIGDSEVGKDVNIGAGTITCNYDGSRKHTTIIEDQVFVGSNTELVAPVRIGRNAVVGAGSTITKDVPEGALAVTRAERIYTKR"
  },
  {
    "reference": "MSWLVGALQTFGSLADVAGTVSNIVYQQRQAAQLEKQNELMETWMNKQEALQKSQMELTRDLSINGPAARVQSALDAGFDEVSARRIAGSGERVIWGNLDRPIMHAGTMDSIRQTRHLDSLSHSLATFKNGTPFGKPAPPTAKSGRPQATTAQITIGHNPGSTSV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.2. The designed protein should have a specific function within the virion component, host cell cytoplasm.3. For general function, the protein need meet that Does not seem to play a role in capsid assembly, but is essential for production of infectious virus .; Minor structural protein present in one or two copies per virion The designed protein sequence is ",
    "response#1": "LPKPPDSDSQRLNAFPVIMAQEGKGRLLRQIRLRKILSGDPSDQQITFVNTYGFIRATPETSEFISESSQQEVTPVVTACMLSFGAGPVLEDPQHMLKALDQTDIRVRKTASDKEQILFEINRIPNLFRHHQISADHLIQASSDKYVKAPAKLIAGVNYIYCVTFLSVTVCSAV",
    "response#2": "FKVPRPILNFRSTRMRGIHLEIIMKITCSENSPIRKTLITDDPENGPKASVWIHLCNLYKGRNPIKVYDEAYFAEKCKQMLLSVGISDLWGPTIAVHANGKIPKSASLYFNSRGWALHPIADASPTMAKQLWSIGCEIIEVNAILQGSDYSALVDHPDVIYN",
    "response#3": "YDIPRHWPEFRSMNAIAFNSLVTIRFEQSPIEHWRILNSEHLLVATFMVHIGNFQRKKTDMYSADYCKPKIEKMGLVFALGGIGGTSLHIRATGKMSKTLWALEGFKKHLCYPLMDINEDLNRELWRSKCKIVRIQAVLQPSVPQDFRVYRDVIIP"
  },
  {
    "reference": "MRADPAAFDAALARRGMAAQAEALLAIDTARRDALTALQGMQSRRNALSKAVGEGRRRGDDTAPQEAEATALRAEMEALERRAAEGEAAIDEALAVLPNILDPDVPEGADETANVVLHVHGEPADPVASPREHFELGEALGLMDFAGAAKLAGARFTVLRGALARMERALGQYMLDTHTTEHGYTEVLPPSLVNDATMYGTGQLPKFADDLFRTTDGRWLIPTAEVPLTNLAAGEILAETALPLRMTALTDCFRSEAGAAGRDTRGMLRQHQFRKVELVSIAHPEASADEHERMTLCAETVLARLGLTYRRVLLCAGDTGFSAAKTYDLEVWLPGQRAWREISSCSNCRAFQARRMNARFRARDGKTLAHVHTLNGSGVAVGRALIAVMETYQQDGGAIAVPDVLRPYMGGLARIERPEN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Incorporate a signal peptide in the protein design.2. The designed protein have Aminoacyl-transfer RNA synthetases class-II family profile domains with specific sequence motifs that are crucial for its activity.3. A protein with ATP binding functions is required to enable selenocysteinyl-tRNA(Sec) biosynthetic process. The designed protein sequence is ",
    "response#1": "NVPIKFWGKFKVYEGDVQEFLKQIKDAKVNYEVIKWKPKGHAEMLEDVLHLGNTLKAAEIAGSRFYYLFNDIVWLDFSLLLFAIDYITQQGYTLVLPPYMLRGEVIQSVIDLDTFKDAIYKIENEDLYLIATAEHPIAAMFFKEEIEKDKLPLKFAGISPAFRKEASAANKDLKGIFRVHQFHKVEQFIFSTPEDSWKYHSELITNAESIFQQLELPYRIVNIASGDLGACAAKKFDLEVWMPAQAKFREMVSCSNCTDWQAFRMKIRYVDRKNNRRGYVHTLNSTAIASTRTITAILENYQREDGVVEVPKVLRKYIY",
    "response#2": "NVPIKFWGKFKVYEGDVQEFLKQIKDAKVNYEVIKWKPKGHAEMLEDVLHLGNTLKAAEIAGSRFYYLFNDIVWLDFSLLLFAIDYITQQGYTLVLPPYMLRGEVIQSVIDLDTFKDAIYKIENEDLYLIATAEHPIAAMFFKEEIEKDKLPLKFAGISPAFRKEASAANKDLKGIFRVHQFHKVEQFIFSTPEDSWKYHSELITNAESIFQQLELPYRIVNIASGDLGACAAKKFDLEVWMPAQAKFREMVSCSNCTDWQAFRMKIRYVDRKNNRRGYVHTLNSTAIASTRTITAILENYQREDGVVEVPKVLRKYL",
    "response#3": "DNVEIRKWGKPREFDFTPKAHYEVAEDLGILDFERAAKVTGSRFVFYKGLGARLERACYNYMLDLHSEEHGYLEVIPPYMTNEASMFGTGQFPKFKEDVFQLEDERHFTLIPTAEVPLTNYYRDEILNEADLPIYFTAMSPSFRSEAGSAGRDTRGLIRMHQFHKVEMVKFSKPSESYDELEKMTDDAESVLRNLKLPHRTIVLSTGDMGFSAAKTYDVEVWVPAQDTYREISSCSNTEDFQARRAMIRYRSEETGSIAYLHTLNGSGVAVGRTVTAILENYQNEDGSITVPDVLVPYTG"
  },
  {
    "reference": "MKVITEVELEHMEYIDFNNEELKSVVAQKLQIQVIVGLFIVMVVLILLSLVSWFNYRSLLSKYYDSKQKNLKKPSSQLANPQFTPITKDLDMKIRRY",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be expressed specifically in the membrane2. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "MXSXGGXXM",
    "response#2": "M",
    "response#3": "MPL"
  },
  {
    "reference": "MNAARTGYRVFSANSTAACTELAKRITERLGAELGKSVVYQETNGETRVEIKESVRGQDIFIIQTIPRDVNTAVMELLIMAYALKTACARNIIGVIPYFPYSKQSKMRKRGSIVCKLLASMLAKAGLTHIITMDLHQKEIQGFFCFPVDNLRASPFLLQYIQEEIPNYRNAVIVAKSPDAAKRAQSYAERLRLGLAVIHGEAQCTELDMDDGRHSPPMVKNATVHPGLELPLMMAKEKPPITVVGDVGGRIAIIVDDIIDDVESFVAAAETLKERGAYKIYVMATHGILSAEAPRLIEESPIDEVVVTNTVPHELQKLQCPKIKTVDISLILSEAIRRIHNGESMAYLFRNITVDD",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Seems to play a negative regulatory role in 5-phosphoribose 1-diphosphate synthesis..2. The protein must exhibit kinase binding. The designed protein sequence is ",
    "response#1": "ASPFLLQYIQEEIPDYRNAVIVAKSPASAKRAQSFAERLRLGIAVIHGEAQDAESDLVDGRHSPPMVRSVAAIHPSLEIPMLIPKEKPPITVVGDVGGRIAIIVDDIIDDVDSFLAAAETLKERGAYKIFVMATHGLLSSDAPRLIEESAIDEVVVTNR",
    "response#2": "AEPQIIKYIRKYIPGEKVIVSPDAGGVKRAKSISDKLDADLAIIHKERKKANEVSGMILVGDVKDKVALIVDDMADTCGTLVSACEMLISKGATKVYALVTHGVLSGDAIQKLNESSLTELVITNG",
    "response#3": "ASPFLLQYIQEEIPDYRNAVIVAKSPASAKRAQSFAERLRLGIAVIHGEAQDAESDLVDGRHSPPMVRSVAAIHPSLEIPMLIPKEKPPITVVGDVGGRIAIIVDDIIDDVDSFLAAAETLKERGAYKIFVMATHGLLSSDAPRLIEESAIDEVVVTND"
  },
  {
    "reference": "MQRLIQYFGLSTCLPQLQQIKLAQMRLDSRTVKEGDLFIGLRGHQQDGRQFIPQAIKAGAKAILTESETENDDLQLDWQQAVPILSVYQLGKKLSAIAGYFYAQPSSQLCLVGVTGTNGKSTIVNLLAQWVTLLGKQAAVMGTIGNGLYGQVEATENTTGSAVEIQANLAQFVEQQAEFVAMEVSSHGLVQGRVEALSFDLAIFTNLSRDHLDYHQDMTSYALAKKRLFSELNTKAQVINIDDPIGKQWLSDFPSAVAVSQFPLDPDIMTKDRLWVVADKIVYTDQGAIISLQSSWGKAELTSHLIGEFNVQNLLLAFAALLKLGLPFEQLVATVAQLQGVCGRMEKFSFSKRPLVLVDYAHTPDALEKALLASRLHCQGKLWCVFGCGGDRDRGKRPLMAQIAEKYADWVVLTDDNPRTEDPRQIMQDLQQGISQMEKVVVEHQRQQALAYAINHADSNDVILVAGKGHEDYQIIGTVKHRFSDRETVMALLSSQA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should contain one or more Mur ligase N-terminal catalytic, Mur ligase C-terminal, Mur ligase central domains that are essential for its function.2. The protein design should take into account the physiological conditions of the peptidoglycan biosynthesis, Cell wall biogenesis pathway.3. The designed protein should have a high specificity for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding, with minimal off-target effects.4. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.5. Incorporating a Meso-diaminopimelate recognition motif motif is critical for the successful design of the protein.6. The designed protein should have UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity to facilitate cell wall organization, peptidoglycan biosynthetic process, cell cycle.7. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MRLADLLAGLDWQGLSGDLENRLELDSRQVQPGDTFVAIIGHQVDGRDYIASAASKGANLVLAQADEWPEGAPSMPVATLDGRRLAGMLANRFYGHPSQQLRLVGVIGVTGTNGKTTTSHLIEQILQAAGIRTGLIGTIENDIGGKRVPAPLTTPESVDLQRHLAAMRDAGCDYVVMEVSSHALDQHRVGGVHFDAAAFTNLSRDHLDYHGTMEAYGAAKAKLFEGLPAGQRPKLAVINADDPHSDAIIAGLPAGRLIRYGRDTAADVRAEHIDVGAEGIRATLVTPWGEGEVRSRLIGRFNVYNVLAAAGAGLAEGLTLEEIAAGLQSLTPVAGRMERVEAGQRFTAIVDYAHKPDALRAVLDSLRGLSPGRLVLVIGAGGDRDPGKRPLMGAVGAELADVLVVTDDNPRTEDPATIRAAVLAGAAAQDAGTEVVEIGDRAEAIARAVALAGDDDVVVVAGKGHEDYQIIGGTRLHFDDREIARAALAGRV",
    "response#2": "MSLRDLFRELEYDVLAGDLHKDNRLELDSRQVQPGDTFVAIIGHQVDGRDYIASAASKGANLVLAQADEVAPDADWIGVEDTRAALARLAARFYGQPSRKLKLVGVTGTNGKTTVTYLLEEIFKQQMKKTGVIGTIQLKIGEESYSISNTTPDALSLQRTFHKMLGENVDQAIMEVSSHALDMGRVYGCDYDIAVFTNLSQDHLDYHSDLQDYLRAKSLLFSQLGNSYDEKKRKFAILNSDDAASDLLKRSTAQHVMTYGCNNKATVMANGIQLDAAGTSFTLTSPVGDIEISSRLIGMFNVYNMLAASTAYGVLTGHDWDAIVRGIASFTGVPGRFELVPNSRDISVIVDYAHTPDSLINVLKTVRPLTKGRVWVIAGCGGDRDKTKRPLMAQAACQFSDEAVFTSDNPRSEDPQRILADMEAGVENQDYTVMEDRKEAIFHAISMARPGDAVVIAGKGHETYQIIGDTKFHFDDREVVREYFELSTDTRSLQPGDLFLALQGERFDGHDYLDTAQRAGARAAIVAHPVAGSTLTQIVLGDTRQALQRMARLHGPTVAVVSNLRVTGTNGKTSVAHFVRQIWTRLGHRAGSLGTTGVDAPGLHGEGGLTTPDPADLHEMLDKMAENRVDRLAMEASSHGLDQYRLDGVRVTAAGFTNLSRDHMDYHGDEQSYLAAKMRLFGEVMAPGGWAVLNTDIPQFEALQALCAERRHRLLTYGRCAVDLRLVKAKPVSGGQSLTLNILGTRFEILLPLAGDFQVANALCALG",
    "response#3": "MKLKDLINKIKIKYVVGSTSVEVEEIAGLACDSRLVAKDQVFFAVPGTKADGLTYAPEAVRRGALAVVTEQSASVPFETAALVKVADVRSALAIASARFYDNPSRKLKVVAVTGTKGKTTTTYMMREILKKQGIKTGLVGTIAIYSGDKKLKDSDRTTPESLELQRMFAQMAEDGCEVVIMEVSSQSLKLSRVAGCNFDIGVFTNFSEDHISPKEHPDMEDYLNSKLKLFNICKKAFVNVDNIYASRILNSIKNCDVKTYGIDNAADLLARDITITNSYVDFMVKLETRNERVKVGIPGRFSVYNALAAISSAVELGLEYETILDALENVGGVAGRTEIIRGENNIDAIIDYAHTPDALTMLLKSVKEVRMWKKIILVFGCGGDRDREKRPKMGIIACKLSDKVIITNDNPRTEDSEHIIREITQAMKCDYIIVKERAKAIKKAFEIADNGDVIIVAGKGRSELVDNKKGLTIMIDYAHSPESLQNILYAVKSYTRGRVISVFGCGGDRDTTKRAIMGEISGKAADYTIITSDNPRTENPEEIVKQVEEGIKRTKGKYVCIVDRKKAIEHAIKMANKNDIIVLAGKGHETYQIIGDTVIPFDDRKVAQEAIIRRIG"
  },
  {
    "reference": "MLYCPPNITLSQVWVDGGTSQCFMDTVSNSITAGWLLLFGTSQLWIYRKWGTQVSQASLPKSQLYGLQLLFLVLLPVLCVVRFAVQATLINDNVVYGYMILSMVLSIVQFPLSIALLVVERHYLLPSVPSRGHGLVLLLFWSLLFIFENLSFVNLRKDDWWFHLTTLTDKIEMALFVLRYVSCLGIFVLGLRAPGIMTYRDYYNIGSASSEARIPIVEDPDSSNVSTWRNFWKKVKILAPFLWPHKEILLQFRVLFCFLLLAGGRVINLYVPIFSKLIVDSMTEKSPIFHYDYVMIYVLFKFLQGSGTGAMGLLNNLRSFLWIRIQQYTTREVEVSLFEHLHALSLRWHLSRKTGEVLRVMDRGTDSINNLLNYILFSIFPTITDIVIAVVFFVSSFNGWFGLIVFTTMVLYIAATIMVTEWRTKFQRRMNLANNAQNARSVDSLLNFETVKYYGAEAYEVEAYKEAILVYQKEEWKNSLTLNILNTAQNLIISAGLLAGSLLCVHMVVDLHALTVGDYVLFSSYIIQLYVPLNWFGTYYRAIQKNFVDMENMFDLLKETQEVVDIPGAGPLAVRQGKIEFCNVSFSYLPERTVLKNISFTVPGGKTVAIVGPSGSGKTTIMRLLFRFYDVDNGAILIDGQNIKTVQQNSLRQNIGVVPQDTVLFNNSIRYNIQYARLNAAEADVIAAAKSAEIHDRILTFPEKYDTQVGERGLKLSGGEKQRVAIARTILKSPAIVLLDEATSALDTQTERNIQAALHKVCADRTTV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein localizes to the melanosome membrane.2. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "MTF",
    "response#2": "GAPGTR",
    "response#3": "MPQAPRL"
  },
  {
    "reference": "MSTNVADLLHTIKNNWCDRSILVVGDVMLDQYIWGDVGRISPEAPVPIVRATHRTEQPGGAANVALNIARLGARATIVGFTGTDDNERALKDYLSSNRVEADFVSCEGFPTITKLRILSGRQQMLRLDNERAESRPSTAYQKLIERALHHLPQSDALILSDYAKGVLLPEVCQTLIQAAAQRKIPVLVDPKNVDFSRYRGATTISPNLGELALAARVDLENLNDLLYAAQQMVRNLGLSFLTATLGEKGIALVTRDKTTISAAVARQVFDVSGAGDAVIATLSLSLASGLDPELGVHLANLAGAIVVSKVGTAPVEQYELLNALTAESVPVAQAKVVTRSELLELVARWRRNDERIVVTNGCFDLLHVGHISLLEQARGFGDRLVVAINSDRSVRELKGNSRPIVGEQERARVLAAIAAVDAVVIFDERTPLELIEATRPDVLVKGGDYAVSGVVGAEEVQSWGGHVKIVPIVEGFSTTKLIEKGH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should take into account the physiological conditions of the ADP-L-glycero-beta-D-manno- heptose biosynthesis pathway.2. The design of the protein must focus on the precise arrangement of amino acids in the active site to enable effective catalysis of ATP + D-glycero-beta-D-manno-heptose 1-phosphate + H(+) = ADP-   D-glycero-beta-D-manno-heptose + diphosphate.3. The designed protein must possess phosphotransferase activity, alcohol group as acceptor to support ADP-L-glycero-beta-D-manno-heptose biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "MPRFDQSRVLVVGDLMLDRYWHGNTSRVSPEAPVPVVDVKQIEDRPGGAGNVALNISALGSASVLAGLVGRDEAAGVLQQRLEAANILCQFQVSETHPTITKLRVISRHQQLLRLDFENPFAPEDAQALSQTALTALKDAKALVISDYAKGALQDPQPLIQAARAQGIAILVDPKGTDFRKYRGATLLTPNLHEFEAIVGKTTSEQDLVEKAQSMIHELELQALLVTRGEHGMSLIRPGEPEFHLPARAREVFDVTGAGDTVISVLAAALGAGDDLPNAVALANLAAGIVVGKLGTAAISAPELRRAIAKQWRSRGRKIVFTNGCFDILHAGHVQYLQKAKACGDCLIVGLNSDASVRRLKGELRPINSQEDRALVLSGLEAVDYVVVFDEETPYELIRAIEPDVLVKGGDYEGKEVVGSDLVKEVKLIEFLEGRSTSAVVKKIL",
    "response#2": "MPRFDQSRVLVVGDLMLDRYWHGNTSRVSPEAPVPVVDVKQIEDRPGGAGNVALNISALGSASVLAGLVGRDEAAGVLQQRLEAANILCQFQVSETHPTITKLRVISRHQQLLRLDFENPFAPEDAQALSQTALTALKDAKALVISDYAKGALQDPQPLIQAARAQGIAILVDPKGTDFRKYRGATLLTPNLHEFEAIVGKTTSEQDLVEKAQSMIHELELQALLVTRGEHGMSLIRPGEPEFHLPARAREVFDVTGAGDTVISVLAAALGAGDDLPNAVALANLAAGIVVGKLGTAAISAPELRRAVEDARAHGEQIVFTNGCFDIIHAGHVGYLEQARKLGDRLVLAINGDESVTRLKGEGRPINPVERRMAVLAGLEAVDWVVSFNEDTPESLLEQLKPDVLVKGGDYGINEVVGADIVSAYGGEVAVLDFLENCSTTAIVQKIRQQ",
    "response#3": "VLCIGDIMLDRYVWGAVERISPEAPIPVLRIERETAMLGGAGNVANNLAALGGGGTFIGVIGDDAPGREIADIMADLTTINAHLLTDTDRRSTIKTRFVGAHQQMMRADVESTQALPASLEVQILERLTHPSGTLGAIILSDYGKGVLSDTIVRGVIEYAHATATTVIIDPKGTDYSRYRGADIITPNKKELMDATGLPAHTDEQVIAACTHLMDTCGVGGVLATRSAEGMTLVLRGQEPHHLKAQAREVFDVSGAGDTVVATLALALASGADSLTAAYLANIAAGIVVAKSGTATVSAPEALVIGDLMLDRYLIGEVQRISPEAPVPVVLLKERNERAGGAANVAANLALLGIPTRICGVVGHDVEAQTLLALMQTANIGTTGVMHSGERPTITKTRVLGGIQQMMRLDQESSAAFSADEKQQLQQQVDDLLAQQPAMIILSDYAKGVLGAELAQHVIKHAKQHGIPLLVDPKGRDYSKYRGATALTPNKRETAEACNVSGQDNAALLQAAAELRKTLALDFLAVTRGEEGISMIEADATSHIPATAKQVFDVSGAGDTVIATLAAGMMHGLPPHQALQLANIAAGIVVGKVGTVPISREELLNLWEA"
  },
  {
    "reference": "VDDTTPLSEETMMRIGGYMV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Agglutinates both native and trypsin-treated rabbit erythrocytes but not human erythrocytes irrespective of blood group type.; Gal / GalNAc-specific lectin The designed protein sequence is ",
    "response#1": "GSERDVQPKPIRMVSMTVSSGAIVDAIAFTYVGTDNVQHSSGIKWGGTGGTEDTINLDATNYVTEISGTVGKFGTDDIVTSLKIITSKGVTRTYGSGTGIPFRVPVLDGGKIAGFFGRAGAFLDAIGFYII",
    "response#2": "GSERDVQPKPIRMVSMTVSSGAIVDAIAFTYVGTDNVQHSSGIKWGGTGGTEDTINLDATNYVTEISGTVGKFGTDDIVTSLKIITSKGVTRTYGSGTGIPFRVPVLDGGKIAGFFGRAGAFLDAIGFYIN",
    "response#3": "MNGLCYKIFDELKAWEDAERFCRKYKPGCHLASFHTYGESLEIAEYISDYHKGQAEVWIGLWDKKKDFSWEWTDRSCTDYLTWDKNQPDVYQNKEFCVELVSLTGYRLWNDQVCESKNAFLCK"
  },
  {
    "reference": "MKNELMQRLRLKYPPPDGYCRWGRIQDVSATLLNAWLPGVFMGELCCIKPGEELAEVVGINGSKALLSPFTSTIGLHCGQQVMALRRRHQVPVGEALLGRVIDGFGRPLDGRELPDVCWKDYDAMPPPAMVRQPITQPLMTGIRAIDSVATCGEGQRVGIFSAPGVGKSTLLAMLCNAPDADSNVLVLIGERGREVREFIDFTLSEETRKRCVIVVATSDRPALERVRALFVATTIAEFFRDNGKRVVLLADSLTRYARAAREIALAAGETAVSGEYPPGVFSALPRLLERTGMGEKGSITAFYTVLVEGDDMNEPLADEVRSLLDGHIVLSRRLAERGHYPAIDVLATLSRVFPVVTSHEHRQLAAILRRCLALYQEVELLIRIGEYQRGVDTDTDKAIDTYPDICTFLRQSKDEVCGPELLIEKLHQILTE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The ATP binding site should be stable and able to withstand changes in environmental conditions.2. A protein that can perform protein-exporting ATPase activity, proton-transporting ATPase activity, rotational mechanism, protein self-association in host cell membrane, cytoplasm, type III protein secretion system complex, proton-transporting ATP synthase complex, catalytic core F(1) to support protein secretion by the type III secretion system, proton motive force-driven ATP synthesis.3. We need a protein that can perform ATP + H2O + cellular proteinSide 1 = ADP + phosphate +   cellular proteinSide 2. with high efficiency.4. The design objective is to achieve a protein with strand-helix-strand-strand-strand-helix-strand-helix-strand-helix-helix-strand-helix-strand-helix characteristics.5. The protein must exhibit the following characteristics: May play a critical role in T3SS substrate recognition, disassembly of the effector/chaperone complex and unfolding of the effector in an ATP-dependent manner prior to secretion (Probable); Required for type III secretion apparatus (T3SA) formation, secretion of a subset of SPI-2 effectors and virulence; Releases the effector protein SseB from the T3SS-2 specific chaperone SsaE in an ATP-dependent manner.. The designed protein sequence is ",
    "response#1": "EGQRVGLFAGAGCGKTTLMAELARNMDCDVIVFGLIGERGRELREFLDHELDETLRRRSVLVCATSDRSSMERARAAFTATAIAEAFRARGQKVLLLLDSLTRFARAQREIGIASGEPLGRGGLPPSVYTLLPRLVERAGMSENGSITALYTVLIEQDSMNDPVADEVRSLLDGHIVLSRKDPAINIENSIEFLRPYDHSQAAGRLRRLLAKYNEVETLVQVGEYRQGSDAVADEAIDRIDAIRDFLSQPTDQLSAYENTLELLIAPYDHSQAAGRLRRLLAKYNEVETLVQVGEYRQGSDAVADEAIDRIDAIRDFLSQPTDQLSAYENTLELLEVDSSHYSFACHFKKIVSLYQRNRDLINVGAYISGTDPDLDMAITLIPKLNKFLQQGMLEKSNFLDSKKSG",
    "response#2": "GVRAIDSAILLGEGQRVGLFAGAGCGKTTLMAELARNMGCDVIVFGLIGERGRELREFLDHELDETLRRRSVLVCATSDRSSMERARAAFTATAIAEAFRARGQKVLLLLDSLTRFARAQREIGIASGEPLGRGGLPPSVYTLLPRLVERAGMSENGSITALYTVLIEQDSMNDPVADEVRSLLDGHIVLSRKLAERGHYPAIDVSASIEGQRMGIFAAAGVGKSTLMGMFARGTQCDVNVIVLIGERGREVREFIELILGADGLARSVVVCATSDRSSIERAKAAYVGTAIAEYFRDRGLRVLLMMDSLTRFARAQREIGLAAGEPPTRRGFPPSVFAELPRLLERAGMGESGSITAFYTVLAEDDTGSDPIAEEVRGILDGHLILSREIRGQRLGLFAGTGVGKSVLLGMMTRFTRADIIVVGLIGERGREVKEFIDEILGEEGLKRSVVVASPADDAPLMRLRAAQYCTRIAEYFRDKGKNVLLLMDSLTRYAQAQREIALAIGEPPATKGYPPSVFAKLPKLVERAGNAEAGGGSITAFYTVLSEGDDQQDPIADAARGVLDGHFVLSRRYKSPAVDSSHYSFACHFKKIVSLYQRNRDLINVGAYISGTDPDLDMAITLIPKLNKFLQQGMLEKSNFLDSKKSS",
    "response#3": "GIRSINGLLTIGRGQKIGLFASSGLGKSVLLGMMTRYTQADIVILSLIGERGREVKEFIDSVLTETVLSRSIVISAPSESSVLMQTRGAIYAMRIAEYFRDKNFHVLLIMDSLTRYAMAHREISLSIGELPVSKGYPPSVFSKLFSLIERAGNGKINSGSITAFFTVLTEEEERYDPISESARSILDGHIILSREYAESGHYPAINIENSIPGPAIDVSASIPAIDVLASLTEQEDDAPIIRLINAILGEAIRENASDIHLETFEKRLVVRFRVDGVLREVLEPKRELAALLVSRIKVMARLDIAEKRIPQDGRISLRVGGREVDIRVSTLPSANGERVVLRLLDKQAGRLNLQHLGMSERDRKLMDETVRKPHGILLVTGPTGSGKTTTLYASLTTLNDRTRNILTVEDPIEYHLEGIGQTQVNAKVDMTFARGLRAILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTNSAIGAITRLVDMGIEPFLLSSSMLGVLAQRLVRVLCPACKEPYRADEAECALLGVDPAAPPTLHRARGCGECHQHGYRGRTGIYELVVFDDHMRSLIHNESSEQEMTRHARTSGPSIRDDGRRKVLEGVTTVEEVLRQNNPILTGEAGVGKTAVVEGFALRIVAGDVPPALKDVELRALDVGLLQAGASMKGEFEQRLRQVIEDVQSSEKPIILFIDEAHTLVGAGGAAGTGDAANLLKPALARGTLRTVAATTWAEYKKHIEKDPALTRRFQVVQVKPIGVFMLAGTSGVGKTETALALAEAMYGGEQNVITINMSEFQEAHTVSTLKGAPPGYIGYGEGGVLTEAVRRKPYSVVLLDEVEKAHPDVHEIFFQVFDKGVMEDGEGRVIDFKNTLILLTTNAGTEMIA"
  },
  {
    "reference": "MKSKSIMVQGTASSVGKSILCTGLCRVFYKDGYNVNPFKSQNMSLNSAITCDGGEIGRAQYMQAEASNKVPSVKMNPILLKPNSDRGSQVIINGKVFKNMDAVDYYKFKPQLKKDVSKIYNKLSDESDIIVIEGAGSPAEINLNKEDFVNMGMAKIAKSPVILVGDIDKGGVFASIVGTMMLLKEDERKMVRGVIINKFRGSYEILKPGLKMLEDIIKVPVLGVIPYFNLNLEDEDSATDWSKFNFNSKGDIDVAVIKLPHMSNFTDINPLKMYDDVSIRLIEKVEDLKNPNLIIIPGSKNTIKDMEYLKSSGMNSAILNCHSNGSFVFGICGGFQILGSKIKDPNNIESNITSIEGLNLINSVTEIKTDKTTTLTEARDKIFNCNIKGYEIHMGKTLIDDNNSNFLVINSRNEETCNDIDGAISKDKRVFGTYIHGIFDNSEFTRKFLNLIRKNAGMDEIKETPKDYWEFKNEEYDKLADIIRNNVDMKKLYEIVNEGIDETDN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Nucleophile active site of the designed protein must be optimized for substrate binding and specificity.2. A protein should be able to manipulate the activity of enzymes in the Cofactor biosynthesis pathway, either by activating or inhibiting their function.3. The protein must exhibit the following characteristics: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. The designed protein sequence is ",
    "response#1": "VAVPPRISNHTDFDPLRLHPQVDLVFVGPGQRIPPADLIVLPGSKSVRSDLAFLREQGWEAAIRRHLRYGGKLLGICGGLQMLGGRIADPLGLEGAPGESRGLGLLDIDTVLEAEKQLRNVRGRLALEGAPVSGYEIHAGVSRGAGLDRPALGLDDGRCDGALSEDGQVLGTYLHGLFESPAACDALLALTG",
    "response#2": "KVVVPVLPRISNHTDFDPLRLHPQVDLQFVGPGQPIPAADLIILPGSKSVRSDLAYLRANGWETAVARHLRYGGKVLGICGGLQMLGEQVHDPLGLEGPAGSSDGLGLLAFSTTLEEEKQLRNVRGRLLLEDAQVSGYEIHAGVTTGDGLSNAAVLLDDGRSDGAQSADGQILGTYLHGLFETR",
    "response#3": "VAVPPRISNHTDFDPLRLHPQVDLVFVGPGQRIPPADLIVLPGSKSVRSDLAFLREQGWEAAIRRHLRYGGKLLGICGGLQMLGGRIADPLGLEGAPGESRGLGLLDIDTVLEAEKQLRNVRGRLALEGAPVSGYEIHAGVSRGAGLDRPALGLDDGRCDGALSEDGQVLGTYLHGLFESPAACDALRIAVIRLPHISNFTDFELLEQYAAVEYVPCGGSLAGYDCIIIPGTKNTIEDLAALREAGTDREILSAREQGIPVIGICGGYQMLGATLIDDGFESAAGEYPGLGLLDCSTRFATYSKNTMQVKRLAAPVSPILVSMGEVTGYEIHMGVTDPGTDQEAFSGDGRVTADGLVFGTYMHGLFLNDPQVLAALRISNHTDFDPLRLHPQVDLQFVGPGQPIPAADLIILPGSKSVRSDLAYLRANGWETAVARHLRYGGKVLGICGGLQMLGEQVHDPLGLEGPAGSSDGLGLLAFSTTLEEEKQLRNVRGRLLLEDAQVSGYEIHAGVTTGDGLSNAAVLLDDGRSDGAQSADGQILGTYLHGLFETAAACSALRDPVSWLSSL"
  },
  {
    "reference": "MLVVVSLTPPVGVCVGLFHHLLSLGGGITTCITSMETGITKWSGSLRCNSQMQKEKGERKGKEEERKRGKEEKIWFHSKKMKIHDYCIVLYCILFYFYFVLILFYFIALYFILHPFYSTILFFFPLFIKCSHLHTLTSFYSLLSSLFSSLIPKHSLHLAPLRKLNLSHFVSPLCAMFPHVGLRLLQTTQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The designed protein should be expressed specifically in the membrane The designed protein sequence is ",
    "response#1": "RGVTILKGQGSYTKEDIDVLYCVVPKNELVMLKSVINSIDPHAFVAVSDVHDVLL",
    "response#2": "RGVTILKGQGSYTKEDIDVLYCVVPKNELVMLKSVINSIDPHAFVAVSDVHDVLF",
    "response#3": "MPTGIKL"
  },
  {
    "reference": "MSLGIDIHVKESVLCIRLTGELDHHTAETLRKQVSGHLEQTDIRHIVMNLADLSFMDSSGLGVVLGRYKEIKQLGGEMIVCAISPAVKRLFDMSGLFKIIRLEQSEQRALETLGVAS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design must be able to perform antisigma factor binding, anti-sigma factor antagonist activity in order to contribute to sporulation resulting in formation of a cellular spore. The designed protein sequence is ",
    "response#1": "MKLKFYLVYLWYKVLLKLGIKTDEIYYIGGSEALPPPLTKEEEEVLLNKLPKGDQAARSLLIERNLRLVVYIARKFENTGINIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMHLRRNNKNRSEVSFDEPLNIDWDGNELLLSDVLGTDDDIITKDLEATVDRHLLMKALHQLNDREKQIMELRFGLAGGEEKTQKDVADMLGISQSYISRLEKRIIKRLRKEFNKMA",
    "response#2": "MVTGVFAALGFVVKELVFLVSYVKNNAFPQPLSSSEEKKYLELMAKGDEHARNMLIEHNLRLVAHIVKKFENTGEDAEDLISIGTIGLIKGIESYSAGKGTKLATYAARCIENEIVITKGGCIHPSLIRFNIYGVRIHNGNFFHDKVNNCFFIFKSMPPLFVMNNEILMHLRALKKTKKDVSLHDPIGQDKEGNEISLIDVLKSENEDVIDTIQLNMELEKVKQYIDILDDREKEVIVGRFGLDLKKEKTQREIAKELGISRSYVSRIEKRALMKMFHEFYRAEKEKRKKAKGN",
    "response#3": "MSLFAAIGYMVREVFVFVSYVKNNAFPQPLSSDDERKYLELMEQGDAQARNLLIEHNLRLVAHIVKKFENTGEDAEDLISIGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRVLKKTKKDVSLHDPIGQDKEGNEISLIDILKSESEDVIDMIQLSMELEKIKEYIDILDEREKEVIVKRFGLGLDKEKTQREIAKALGISRSYVSRIEKRALMKMFHEFVRAEKEKKAKV"
  },
  {
    "reference": "MSDFQHAQLDWDENGQPLSRAFGDVYFSRHSGLNETRHVFLATNRLAERFAALGDGEVLCIGETGFGTGLNFLCAWQLFERVAPAGARLEFVSVEKFPLAAADLRRALALWPELAPWSEALLGQYLAVHPGFQRLAFAGGRVGLTLLLGDALECLPQLDARVDAWFLDGFAPAKNPDMWSPVLFAELARLSAPQATLGTFTSAGFVRRGLVEAGFAMQRVPGYGQKREMLSGTYQGPPANAGKPWYARPAPHAGRRAALVVGGGLAGCASAASLAARGWQVTLIERHPGLAREASGNPQGVLYLKLSAHGTPLSRLVLSGFGHTRRLLERLRRGHDWDACGVLQLAFDAKEAQRQAQLAAAFPADLLHGLEREQAERLAGVALPAGGLFYPEAGWVHPPALCQALAATPGITLLSGRAVRLRREGDDWCAYAGDECLARAPLAILATAADIRDFPPAAELPLKRIRGQVTRLPATPQSRALRTVVCAEGYVAPPRGDEHTLGASFDFKSEDLAPTLAEHQGNLELLREISPDLLQRLGADDLPLERLEGRAAFRCTSPDYLPLVGPLAERAAFDEAYAVLARDARQVPERACPWLPGLYLNSGHGSRGLISAPLSGELLAAWICGEPLPLPRAVAEACHPNRFLLRDLVRGQRG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design should incorporate a binding pocket that specifically recognizes FAD to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "PKSRGTVRLRSADPRVNPMVDPNFLGDPADLETSAEGVRLSYEMFSQPSLEKHIRKTCFFSGKQPTMQMYRDYAREHGRTSYHPTCTCKMGRDDMSVVDPRLKVHGLEGIRICDSSVMPSLLGSNTNAATIMY",
    "response#2": "PKSRGTVRLRSADPRVNPMVDPNFLGDPADLETSAEGVRLSYEMFSQPSLEKHIRKTCFFSGKQPTMQMYRDYAREHGRTSYHPTCTCKMGRDDMSVVDPRLKVHGLEGIRICDSSVMPSLLGSNTNAATIMY",
    "response#3": "ELEVFDYYEQVLDTNWKNWQETNLDLYHEFMHFANRKTGLTVKEYYQRAWKLYPNGHAAIERYRAQYSNYAGWQDRDDGIRLPGLHPNEFQLVNLFPDLAINARGTVIRIDSQTPISPGKTLVQYRGLGLKRDSERERVQRVRDYTSIWGPFGTNLAEDTLATSLHAKTIQTGSVPFTYLTRDEGGMTQDDLGLRTFYREWERLR"
  },
  {
    "reference": "MIDLKLLMNDFEEVAERLAKRHLEADFLEQARLLAGQYKAKKRESEELQAEQNAKSKLFGQYKKEGKEIDSLKAELDTLKIRLSEILPEVSVLERELESLALIIPNLPDEKTPIGRDEHDNIEIRRVLEPKMFDFRPKEHWELAEQNGWIEFDRGVKLAKSRFSVLRKEGARMSRALISYMLDFNHSRGFEEVVTPVIVNRETLLGTGQLPKFENDLFKISEVIEEEQGGEKGRGHELYLIPTAEVTLTNLYRDEIIPHDELPLLLTAHTPCFRKEAGSAGRDTRGIIRQHQFDKVELVALTKPEESDAIQQKMIDCASDLLSSLGLPHRLVQLCGGDLGFGASNTVDIEVWLPGQNCYREISSISNTRDFQARRAMIRYKDEDKKNRLVHTLNGSSLAVGRTLVAIMENYQNADGTIEIPEVLRRYL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Catalyzes the attachment of serine to tRNA(Ser); Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L- seryl-tRNA(Sec), which will be further converted into selenocysteinyl- tRNA(Sec)..2. The protein should be designed to have high stability and activity towards ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L-   seryl-tRNA(Sec).3. The protein design must be focused on enhancing the efficiency of selenocysteine biosynthetic process, seryl-tRNA aminoacylation. The designed protein sequence is ",
    "response#1": "DNVFLHAWGEKPSFDFTPKEHWEIGPALGLDFERGSRLTGSRFTVLWHWAAKLERALTAFFLDVHTREHGYMEVYPPAMVNAQTMTGTGQLPKFEEDLFKLRDSEYYLIPTAEVPLTNLHSGEVVPEEKLPIAYTAQTQCFRSEAGSYGKDTKGFIRQHQFTKVEMVRFAHPEKSFEELEKLRSHAEVLLQKLGLHYRVVTLCSGDMGFSAAKTYDLEVWLPGQDKYREISSCSNCTDFQARRANIRTRLADAKKPVFLHTLNGSGLAVGRTLVAILENYQQADGSVVVPEVLRPYMGQ",
    "response#2": "MIDQNLLRTNLEEVAQILKLKRNFNLDVARVTSLEEQRKALQVKAENLQAERNARSKNIGAAKARGEDISALLTEVDTMGSELDAAKVELDKVQAEIRELLLNIPNLPHESVPEGEDEDKLLRAITNLMIDLNKSKYQEWNLPVVINELSLRSTGQLPKFKDDVFKLENTRYYLSPTLEVQLINLHANEIFNEEDLPKYYTATGINFRQEAGSAGKQTKGTIRLHQFQKTELVKFCKPENAINELEAMVRDAEQILKALKLPFRRLLLCTGDMGFSAEKTYDLEVWMAASNEYREVSSCSSCGDFQARRAMIRYKDINNGKNSYVATLNGTALSIDRIFAAILENFQTKDGKILIENTRYYLSPTLEVQLINLHANEIFNEEDLPKYYTATGINFRQEAGSAGKQTKGTIRLHQFQKTELVKFCKPENAINELEAMVRDAEQILKALKLPFRRLLLCTGDMGFSAEKTYDLEVWMAASNEYREVSSCSSCGDFQARRAMIRYKDINNGKNSYVATLNGTALSIDRIFAAILENR",
    "response#3": "MLDINRIVQNPEELLSTLQKRGVVSSDIEAKIKSVSEKQRILKLEVEELRAERNRVSKEIGIQKSQGKDITEISNSMKGVGDRIKSIEEELTKEEESLHELNLGLPNVADDTVPVGPDETENLEVSRWGTPRQFDFEIKDHVTLGEGLGGLDFPAGVKLTGSRFVVMKSGIARLHRALSQFMLDLHTEQHGYVETYVPYLVNHDTLYGTGQLPKFGEDLFHTQPLDGQDPNAVQKPYALIPTAEVPVTNLVRDEILDEESLPLKLTAHTPCFRSEAGSYGRDTRGLIRMHQFDKVEMVQIVAPEKSMEALEELTGHAEKVLQLLNLPYRKMLLCSGDMSFGSSKTYDLEVWLPAQNTYREISSCSNMWDFQARRMQARCKAKGDKKTRLVHTLNGSGLAVGRTLVAVLENYQNADGSITVPEVLKPYLGGLEVIGK"
  },
  {
    "reference": "MNKGQRHIKIREIITANEIETQDELVDILKKDGYNVTQATVSRDIKELHLVKVPTNNGSYKYSLPADQRFNPLSKLKRSLMDAFVKIDSASHLIVLKTMPGNAQAIGALMDNLDWEEIMGTICGDDTILIICRTHDDTKVVQKKILELL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Ideal protein should have a binding affinity for key enzymes in the Amino-acid degradation pathway to enhance the overall efficiency of the pathway.2. The protein design must be able to perform DNA-binding transcription factor activity in order to contribute to arginine catabolic process to ornithine, arginine biosynthetic process, protein complex oligomerization.3. The protein must exhibit the following characteristics: Regulates arginine biosynthesis genes and activates arginine deiminase pathway genes.. The designed protein sequence is ",
    "response#1": "YSMDRNMLHCSFEGGELEDPWNEPGPNSYVMAVPMEQAPDEAEYISIDFEHGNPVAVNGERLSPAALVKKLNSIGGRHGIGRLDMVENRFVGIKSRGVYETPGGTLIHIAHRDLEGICIDRETMHLRDAMLPRYAAAIYNGFWFAPEREAMQAMIDVSQQRVTGTVRLKLYKGNAWPVGRQSPNTLYCHDLATFEDCATYDHKDAAGFIKLQGLRIAA",
    "response#2": "YSMDRNMLHCSFEGGELEDPWNEPGPNSYVMAVPMEQAPDEAEYISIDFEHGNPVAVNGERLSPAALVKKLNSIGGRHGIGRLDMVENRFVGIKSRGVYETPGGTLIHIAHRDLEGICIDRETMHLRDAMLPRYAAAIYNGFWFAPEREAMQAMIDVSQQRVTGTVRLKLYKGNAWPVGRQSPNTLYCHDLATFEDCATYDHKDAAGFIKLQGLRIL",
    "response#3": "YSKDENSWHISTEGGLLENPWNQSNEDCWSWTVNPEDAPEKPEYVSLQLKEGCVVSVNNQKLNPLKCVEELNSLGAKHGIGRIDIIENRLIGMKSRGCYETPGGTIIMTAIKAIEQLVLDRESFRWREKIGLEMSSIVYDGRWFSPIRKSLQAAADSLSLEITGEVILKLYKGSVTAVQKKSPNSLYSEEYATFGEDKVYKQSDADGFIRLFSLSSKV"
  },
  {
    "reference": "MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEATFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGNAGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIGGGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAIGLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYHTEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDIAFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPLDGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPELVVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRALNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQRYFLF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be optimized for urease activity activity in order to improve urea catabolic process.2. We are looking for a protein with broad substrate specificity for 2 H(+) + H2O + urea = CO2 + 2 NH4(+).3. The designed protein should have a binding site that is specific to the target substrate, Ni(2+) ligand.4. The protein must be engineered to have a high affinity for Ni cation in order to increase its catalytic activity.5. The designed protein must have at least one Urease domain.6. The protein design should consider the endogenous proteins and enzymes present in the CO(2) and NH(3) from urea (urease route): step 1/1, Nitrogen metabolism pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "GGIDTHVHFINPEQAYVALESGVTTHIGGGTGASEGAKATTVAPGPWHIHRMLEAAEGLPINVGFTGKGQAHNHTALIEQIHAGAIGLKVHEDWGATPSALSHALDVADDYDVQIALHADTLNEAGFMEDTMKAIKDRVIHMYHTEGAGGGHAPDLIKSASYPNVLPSSTNPTLPYTVNTIDEHLDMVMITHHLNASIPEDIAFADSRIRKETIAAEDVLQDMGVFSMVSSDSQAMDRVGEVITRTWQVAHRMKEQRGYLDGDKEYNDNNRIKRYIAKYTINPAITHGISEYVGSIEEGKLADLVIWDPAFFGVKPEMILKAGMINTAVNGDANGSIPTSEPLKYRKMYGQYGGNLTGTSITFVSNIAYMNDIERQLSLHRMVRPVKGIRQLTKKDMKNNSETPKLDVDPQTYEVFVDGKLITSEPAKELPLAQRYFLGGIDTHIHFICPQQIDEALTSGVTTMIGGGTGPATGTFATTVTPGPWHMERMLQAVDGYPMNIGLLGKGNASQAAPLREQIAAGAIGLKLHEDWGSTPAAIDCCLGVADETDTQVAIHTDTLNEGGFVEATVAAFKGRTIHTYHTEGAGGGHAPDIIKVCGEANVLPSSTNPTRPYTVNTLDEHLDMLMVCHHLDASIAEDIAFAESRIRRETIAAEDVLHDLGAFSMISSDSQAMGRVGEVVLRTWQTAHKMKVQRGSLPGDNARHDNFRVKRYVAKYTINPARTHGIAHVVGSVEVGKLADLVLWRPAFFGVKPSLILKGGSIASAAMGDPNASIPTPQPVHYRPMFAGQGSGVAAASLTFVSQAAQQAGVADRYGLRKRTVAVENCRSVSKRDMVHNDWQPDISVDPETYQVVADGQLLTCEPATELPMAQRYFL",
    "response#2": "GGMDSHVHFICPQQIEEALASGITTMYGGGTGPSTGTNATTCTPNKDLIRSMLRSTDSYPMNIGLTGKGNDSGSSSLKEQIEAGCSGLKLHEDWGSTPAAIDSCLSVCDEYDVQCLIHTDTLNESSFVEGTFKAFKNRTIHTYHVEGAGGGHAPDIISLVQNPNILPSSTNPTRPFTTNTLDEELDMLMVCHHLSRNVPEDVAFAESRIRAETIAAEDILQDLGAISMISSDSQAMGRCGEVISRTWKTAHKNKLQRGALPEDEGSGVDNFRVKRYVSKYTINPAITHGISHIVGSVEIGKFADLVLWDFADFGARPSMVLKGGMIALASMGDPNGSIPTVSPLMSWQMFGAHDPERSIAFVSKASITSGTLSSPYEGGIDTHIHFICPQQIDEALTSGVTTMIGGGTGPATGTFATTVTPGPWHMERMLQAVDGYPMNIGLLGKGNASQAAPLREQIAAGAIGLKLHEDWGSTPAAIDCCLGVADETDTQVAIHTDTLNEGGFVEATVAAFKGRTIHTYHTEGAGGGHAPDIIKVCGEANVLPSSTNPTRPYTVNTLDEHLDMLMVCHHLDASIAEDIAFAESRIRRETIAAEDVLHDLGAFSMISSDSQAMGRVGEVVLRTWQTAHKMKVQRGSLPGDNARHDNFRVKRYVAKYTINPARTHGIAHVVGSVEVGKLADLVLWRPAFFGVKPSLILKGGSIASAAMGDPNASIPTPQPVHYRPMFAGQGSGVAAASLTFVSQAAQQAGVADRYGLRKRTVAVENCRSVSKRDMVHNDWQPDISVDPETYQVVADGQLLTCEPATELPMAQRYFL",
    "response#3": "GGMDSHVHFICPQQIEEALASGITTMYGGGTGPSTGTNATTCTPNKDLIRSMLRSTDSYPMNIGLTGKGNDSGSSSLKEQIEAGCSGLKLHEDWGSTPAAIDSCLSVCDEYDVQCLIHTDTLNESSFVEGTFKAFKNRTIHTYHVEGAGGGHAPDIISLVQNPNILPSSTNPTRPFTTNTLDEELDMLMVCHHLSRNVPEDVAFAESRIRAETIAAEDILQDLGAISMISSDSQAMGRCGEVISRTWKTAHKNKLQRGALPEDEGSGVDNFRVKRYVSKYTINPAITHGISHIVGSVEIGKFADLVLWDFADFGARPSMVLKGGMIALASMGDPNGSIPTVSPLMSWQMFGAHDPERSIAFVSKASITSGHLARKRFGQNFLGGMDSHVHFICPQQIEEALASGITTMYGGGTGPSTGTNATTCTPNKDLIRSMLRSTDSYPMNIGLTGKGNDSGSSSLKEQIEAGCSGLKLHEDWGSTPAAIDSCLSVCDEYDVQCLIHTDTLNESSFVEGTFKAFKNRTIHTYHVEGAGGGHAPDIISLVQNPNILPSSTNPTRPFTTNTLDEELDMLMVCHHLSRNVPEDVAFAESRIRAETIAAEDILQDLGAISMISSDSQAMGRCGEVISRTWKTAHKNKLQRGALPEDEGSGVDNFRVKRYVSKYTINPAITHGISHIVGSVEIGKFADLVLWDFADFGARPSMVLKGGMIALASMGDPNGSIPTVSPLMSWQMFGAHDPERSIAFVSKASITSGTIAAPADLAACFGTPVRVM"
  },
  {
    "reference": "MIRSRVNLAREVPKKAKAHLSKERRLIKEDSEFVFGTHSVKNALATRKRECRALYVQNADIHSEFEEFLNKLQYKIPIKSVNKEHLNQITACRPHNGVVLEASSLNVPTISDLLFPAGEEYNNKNGQDSPHNDLNEGKSSSSDNYPPLYVYVDGITDPQNMGAVIRSAYILGAKGILLSKKHNTFLSPVVSKASAGALEVFNISHVKNPMVFLRNSVLKGWKVIGTKPALPDNKDQIYTPHKIKTELMNEPKILVLGSEKGLRTNILTQCSHYVSIPGGDKYVDSLNVSVAAGILLYSLVN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein can be expressed in mitochondrion and perform RNA binding without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "DSGAGAGIQADLKVCAARGVYCASVITAVTAQNTRGVQSVHLLPPEFISEQLKSVLSDFEFDVVKTGMLPSTEIVEVLLQNLSDFPVRALVVDPVMVSTSGHVLAGSSILSIFRERLLPIADIITPNVKEASALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGGDLPDSSDSVDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSMLSAVKVAKRFVDNALDYSKDIVIGSC",
    "response#2": "DSGAGAGIQADLKVCAARGVYCASVITAVTAQNTRGVQSVHLLPPEFISEQLKSVLSDFEFDVVKTGMLPSTEIVEVLLQNLSDFPVRALVVDPVMVSTSGHVLAGSSILSIFRERLLPIADIITPNVKEASALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGGDLPDSSDSVDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSMLSAVKVAKRFVDNALDYSKDIVIGSS",
    "response#3": "DSGAGAGIQADLKVCAARGVYCASVITAVTAQNTRGVQSVHLLPPEFISEQLKSVLSDFEFDVVKTGMLPSTEIVEVLLQNLSDFPVRALVVDPVMVSTSGHVLAGSSILSIFRERLLPIADIITPNVKEASALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGGDLPDSSDSVDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSMLSAVKVAKRFVDNALDYSKDIVIGSC"
  },
  {
    "reference": "MEKLIIQGGQPLVGDVHIEGAKNAVLPIMAAALLASKGPVELTNVPILSDVFMMQDVLKSLDARVKFDEQRNYLMIDANQPLNFEAAFEYVSKMRASIVVMGPLLARLGHARVAMPGGCAIGSRPVDLHLKGFEALGATITQSHGYIEAKADQLVGANIYLDFPSVGATQNIMMAATLAKGTTVIENVAREPEIVDLANVLNKMGAKVFGAGTEEIRIEGVTELKGTEHSIVQDRIEAGTFMIAAAATKGNVLVEEAISEHNKPLLSKLAEMGAQVIEEENGIRIIGPDELKPSNIKTLPYPGFPTDMQAQMTALQLMAHGTSVMTETVFENRFMHLEELRRMNADYQIEGQSVILYGPPELTGAEVAASDLRAAAALVIAGLVANGETLVTNLQYMDRGYYHFHQKLRALGAHISRADFDEAGHRKTTQKLA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein with UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity is needed to enhance peptidoglycan biosynthetic process, UDP-N-acetylgalactosamine biosynthetic process in a specific context.2. The protein must exhibit the following characteristics: Adds enolpyruvyl to UDP-N- acetylglucosamine..3. The protein should be optimized for activity in peptidoglycan biosynthesis, Cell wall biogenesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "GGNELHGVIPISGAKNAALPLMIASLLTDDTLTLENVPHLADVEQLIRILGNHGADISVNGRRERQGESYARTVHFTSRNIVSTTAPYELVSKMRASFWVIGPLLAREGKARVSLPGGCAIGTRPVDLFIEGLAALGANIEIDGGYVNATAPAGGLIGARYVFPKVSVGATHVLMMAATLANGTTVLGNAAREPEVVDLAKCLNAMGAKISGQGTSTVTIEGVRSLSGARHRVLPDRIETGTYAMAVAMAGGDVILEDTEASLLDTALEAIRRAGAQISETNSGIRIVRNGAGIKPVDIVTDPFPGFPTDLQAQFMGLMTRSSGVSHITETIFENRFMHVQELARLGAKISLSGQTAKVEGVSRLKGAPVMATDLRASVSLVIAGLAAEGETMVSRVYHLDRGFERLEEKLRALGARIERI",
    "response#2": "LLVLGGSQGAKAINLLLPQALSLLPEEQRPQVLHQTGARWLEKTQTEYAALNVHAEIVPFIDDMAKAYANADWVIARSGALTVSEIATAGLAALFIPFPYAVDDHQTMNAQCLAEVGAAAILEEKTLTADILATAIQSRQDRSAE",
    "response#3": "LLVLGGSQGAKAINLLLPQALSLLPEEQRPQVLHQTGARWLEKTQTEYAALNVHAEIVPFIDDMAKAYANADWVIARSGALTVSEIATAGLAALFIPFPYAVDDHQTMNAQCLAEVGAAAILEEKTLTADILATAIQSRQDRSAAA"
  },
  {
    "reference": "MDFFSLVFVTGNANKLREVKEILSQGGYPIEIDSQRLEIPEIQGTTIEVATDKCRRAAELVGGPCITEDTALCYVALKGLPGPYIKHFMVAVGHEGLNAMLDGFPTRAAEAVCTFAYSAGPGAEPVIFEGRTEGTIVPARGPKVFGWDAVFEPLGTGMTYAEMPADQKNKISHRYKALDKLRTYLQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. Mn(2+) is required for the proper folding and stability of the protein.2. The protein must exhibit dITP diphosphatase activity. The designed protein sequence is ",
    "response#1": "MQ",
    "response#2": "LWS",
    "response#3": "GILG"
  },
  {
    "reference": "MCPPTIWEKGGQVGARWSLRAPEVSAMAEDGSEEIMFIWCEDCSQYHDSECPELGPVVMVKDSFVLSRARSSLPSNLEIRRLDDGAEGVFAVTQLVKRTQFGPFESRRVAKWEKESAFPLKVFQKDGHPVCFDTSNEDDCNWMMLVRPALEPGHQNLTAYQHGSDVYFTTSKDIPAGTELRVWYAAFYAKKMDKPMLKQACSSVQAAGTPEPSVSVEPERGQWVCKVCSNTFLELQLLNEHLLGHLEQAKSLPAGGQQHEAASEKEPDAPRMEPPTAAESKSIQSVMVTKEPKKKPRRGRKPKASKVEQPLVIIKDKEPSEHVAEIITEIPPDEPVSATPDERIMELVLGKLAAPTNEASSVPKFPHHPSSTIALKRGLVLSSRHGVRRKLVRQLGEHKRIHQCGTCSKVFQNSSNLSRHVRSHGECAHGDKLFKCEECSKLFSRKESLKQHVSYKHSRNEVDGEYRYRCGSCGKTFRMESALEFHNCRTDDKTFQCEMCFRFFSTNSNLSKHKKKHGDKKFACEVCSKMFYRKDVMLDHQRRHLDGVRRVKREDLEASGESLVRYKKEPSGCPVCGKVFSCRSNMNKHLLTHGDKKYTCEICGRKFFRVDVLRDHIHVHFKDIALMDDHQREEFIGKIGISSEENDDNSDESADSEPHKYSCKRCQLTFGRGKEYLKHIMEVHKEKGHGCSICHRRFALKATYHAHMVIHRENLPDPNVQKYIHPCEICGRIFNSIGNLERHKLIHTGVKSHACEQCGKSFARKDML",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "MQARKRKRTSIENRVRWSLETMFLKCPKPSLQQITHIANQLGLEKDVVRVWFCNRRQKGKRR",
    "response#2": "DMKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEAN",
    "response#3": "DMKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEAX"
  },
  {
    "reference": "MKIMSKLPNILLAVTGGIAAYKSAVLARLLIKAGCSVKVMMTAGACEFITPLTFQALTGQAVHTELLDETAELGMGHIELAKWADLVVVAPASANTIAKLAGGFADNLVTTVCVATSAPIMIAPAMNQQMWANAIVQDNLKKLRQFGHHVISPDSGEQACGDVGAGRLPEPEDLCERILAFYARRQIPQSLLGKTVVITAGSTIEPIDPVRFLSNHSTGKMGYALASACRDAGADVILVSGKRVVLQTPYGVKKMTVGTADEMLQTCLSVCQRADVFIATAAVADFKMASVATHKIKKTQGQESMTLELVKNPDVLATICSAYPNLLAVGFAAETQDTENYAKAKLAAKRLDMIAVNDVSDQSIGFGSDDNAMTVFFAERYVESSVDLPKASKANIAEQLVRCIAKIIDKKSL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.2. We require a protein that can specifically bind to the metabolites produced in the CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis, coenzyme A biosynthesis pathway.3. The presence of Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains is critical for the interaction of the designed protein with its intended targets.4. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.5. The protein with the reaction H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2, the Proton donor must be optimized to ensure efficient catalysis.6. The designed protein must possess phosphopantothenoylcysteine decarboxylase activity to support coenzyme A biosynthetic process, pantothenate catabolic process under certain conditions.7. The protein should be able to bind CTP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "AGGIAAYKACELLRRFTESGHDVTVVPTAAALEFVGAPTWAALSGKPVATDVWSGVHQVPHVRIGQQADLVVVAPATADLLAKAAHGLADDLLTNTLLTARCPVVFAPAMHTEMWEHAATRANVATLRERGCVVIEPAEGRLTGKDTGKGRLPEPGEIFEACLAALRRHLLGIGGGISAYKSVDLVRRLKERGFDVRVVMTHAATALVTPLTFQAVSGYPVRTTLLDPAAEAGMSHIELARWADWLLIAPATADLMARLAAGIADDLLTTLALATEAQLCLAPAMNQHMWRHPATQENLEVLRARGARILGPAEGDQACGDVGLGRMLEPLEIAERVLLGVSGGIAAYKAAELVRRLRDAGAEVRVAMTANATHFVGVATFQALSGNPVRTSLWDESAEAAMGHIELARWADEVLIAPASADLLARIAHGHADDLLSTLVLATTAPVSVAPAMNQQMWANPATQANIAQLRERQVRVFGPAVGNQACGETGAGRLPEAQELVAGWLAPGDLLAGRHVLVSAGGTRERLDPVRFLGNRSSGLQGYALARAAAARGAEVTLVSANVALPDPAGVKVVRIETTGELLDAVTAETGSVDAVVMAAAPADFRPARVSEAKIKKAADGSAPAIELVQNPDVLAKISQDRARADQVIVGFAAETGDDTGSVLELGRAKLARKGCDLLVVNDVSGGAVFGSADNEAVILGRDDGSVERVPHGSKREVADAVWDVVGALLE",
    "response#2": "LLGIGGGISAYKSVDLVRRLKERGFDVRVVMTHAATALVTPLTFQAVSGYPVRTTLLDPAAEAGMSHIELARWADWLLIAPATADLMARLAAGIADDLLTTLALATEAQLCLAPAMNQHMWRHPATQENLEVLRARGARILGPAEGDQACGDVGLGRMLEPLEIAELVERLGDAADLAGRHVLVSAGGTRERLDPVRFLGNRSSGLQGYALARAAAARGAEVTLVSANVALPDPAGVKVVRIETTGELLDAVTAETGSVDAVVMAAAPADFRPARVSEAKIKKAADGSAPAIELVQNPDVLAKISQDRARADQVIVGFAAETGDDTGSVLELGRAKLARKGCDLLVVNDVSGGAVFGSADNEAVILSADGRETPVPMGSKDELADTVWDLVAARLG",
    "response#3": "AGGIAAYKACELLRRFTESGHDVTVVPTAAALEFVGAPTWAALSGKPVATDVWSGVHQVPHVRIGQQADLVVVAPATADLLAKAAHGLADDLLTNTLLTARCPVVFAPAMHTEMWEHAATRANVATLRERGCVVIEPAEGRLTGKDTGKGRLPEPGEIFELLGIGGGISAYKSVDLVRRLKERGFDVRVVMTHAATALVTPLTFQAVSGYPVRTTLLDPAAEAGMSHIELARWADWLLIAPATADLMARLAAGIADDLLTTLALATEAQLCLAPAMNQHMWRHPATQENLEVLRARGARILGPAEGDQACGDVGLGRMLEPLEIAESALAALAEGPLKGKRILVTSGPTHEPIDPVRYIANRSSGAQGAALGRALAALGAEVVFVTGPADVPPPEGVEVERVETAREMLDAVQAALPADAAVFAAAVADWRVSSTSDRKLKKSSDGLPQLTFAENPDILRTVSEMTQGRPGLVVGFAAETNDVVDNATAKRARKGCDWIVANDVSPATGIMGGTENAVVLVADAAGEPQEYPRMSKDDVARRLVERIAALRP"
  },
  {
    "reference": "MSEISFVTVTALTKYIKRKFDVDPHLEDIWIKGELSNVKIHSRGHIYFTLKDDNARIQSVMFARQNRNLAFTPENGMKVLVRGGISVYEPSGSYQLYAKEIQPDGIGALHLAYEELKKKLSQEGLFDEKYKKPIPAFPSVVGVVTSPTGAAVRDVITTIKRRYPLVKVIVLPTLVQGVNAGESIVSSIEEANRRDLCDVLIVGRGGGSIEELWAFNEEAVARAIFSSDIPIISAVGHETDFTISDFVADLRAATPTGAAELAVPNMLDLLERLKTLEARMTKSMQQDLKKRQERVKNLQSSYAFRYPKRLYAQKEQEFDLIFDRFQKQLAMTVDRKKQKLNEQTYKLEMLHPEEQLKQARKRHEEQTVRLKRNMKVQLKQIHSQFQTVLGKLNALSPLQVMERGYSLTYKENELIKSIEQVHEKDEIRVTLNDGTLTCEVLQKRGETNG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be involved in DNA catabolic process by localizing to exodeoxyribonuclease VII complex and exhibiting exodeoxyribonuclease VII activity, nucleic acid binding.2. The protein must exhibit the following characteristics: Bidirectionally degrades single-stranded DNA into large acid- insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides..3. We need a protein that can perform Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-   direction to yield nucleoside 5'-phosphates. with high efficiency. The designed protein sequence is ",
    "response#1": "LFDPRLKRPIPFLPNMIGIITGRASAAERDVTTVASARWPAARFAIRNTAVQGPNAVSQIVEALRELDRNVDVEVIVVARGGGSVEDLLTFSDETLCRAIAACRTPVISAVGHEPDNPLCDLVADLRAATPTDAAKKVVPDAAVEQRLIEDLQRRSAQALRNWVSREQRTLVQLRSRPVLAEPLRTLTTRSEEIHRAQSAIRRR",
    "response#2": "LFDPARKRPLPTHVQRLAVITSPTGAAVRDVLSVLGRRFPLLEVDLLPTLVQGSSAAAQITRLLQAADASGRYDVILLTRGGGSLEDLWAFNDEALARAIAASRTPVVSAVGHETDFSLSDFAADLRAPTPSVAAELLVPDQRELALRLRRTAARMVQLQRHAMQQAMQRADRALLRLNAQSPQARLDLLRRRQLDLGRRLHAVFNQQQERRAARLRHAAAVLRGHHPQRQLDAMQRRLAALRGRPQAAMQRLLERDALRLRGLARSLEAVSPLATVARGYSILTRTDDGTLVRKVNQVQPGDALQARVGDGVIDL",
    "response#3": "LFDPRLKRPIPFLPNMIGIITGRASAAERDVTTVASARWPAARFAIRNTAVQGPNAVSQIVEALRELDRNVDVEVIVVARGGGSVEDLLTFSDETLCRAIAACRTPVISAVGHEPDNPLCDLVADLRAATPTDAAKKVVPDAAVEQRLIEDLQRRSAQALRNWVSREQRTLVQLRSRPVLAEPLRTLTTRSEEIHRAQSAIRRR"
  },
  {
    "reference": "MRKNLLDYSLEELYDIDILTPSFRARQLYDLVSVGARWEDVTVLPKQMLEQLKSRFALGVTIKETFVSKDGSAKFLFALDDGNIVEGVFMPHDYGNTVCVSTQVGCRMGCAFCASGIGGLVRNLTAGEILGQVLAFNGFFAEKKGKRAVTNIVLMGSGEPLDNYDEVTKFLRLVSGERGINISQRNISLSTCGIVPKIRQLADDGFSVTLSVSLHATTDDARRQLMPIANKYSITEIIGAVKYYFQKTGRRIIFEYSMVKGSNIDFFDAKRLSELTKGYPSHVNLIMLNPVKEKNVKGCTKAEADRFLERLKNFGVSATIRRSFGADIGGACGQLRRSYVDVSSDKK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design must be able to perform tRNA (adenine-C2-)-methyltransferase activity, rRNA binding in order to contribute to rRNA base methylation.2. The design of the protein must focus on the precise arrangement of amino acids in the S-methylcysteine intermediate, Proton acceptor to enable effective catalysis of adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "SMPPKHFADYTKPERIEILKELGLPKFRDNQIARQYYGRFQADPKKMTDLPESVRERIAETLFPPLMEPVRETSADDGNTQKMLWRLHDGTLLESVLMEYPDRATLCISSQAGCGMACPFCATGQGGLDRNLSTGEIVDQVRAAAATMQAKGGRLSNIVFMGMGEPLANYNRVLSAIHQITQPSPEGFGISQRGITLSTVGLAPAIRRFAKEGLSCRLAVSLHTPDDELRDSLVPANNRWPVEEVLDAAKEYADASGRRVSIEYALIKDINDQPWRADLLGKKLHGALGAKVHVNLIPLNPTPGSKWDASPKRVQDEFQRRVIARGVTCTVRDTRGQEIAAACGQLAAEERSGRRLLAQ",
    "response#2": "SMPPKHFADYTKPERIEILKELGLPKFRDNQIARQYYGRFQADPKKMTDLPESVRERIAETLFPPLMEPVRETSADDGNTQKMLWRLHDGTLLESVLMEYPDRATLCISSQAGCGMACPFCATGQGGLDRNLSTGEIVDQVRAAAATMQAKGGRLSNIVFMGMGEPLANYNRVLSAIHQITQPSPEGFGISQRGITLSTVGLAPAIRRFAKEGLSCRLAVSLHTPDDELRDSLVPANNRWPVEEVLDAAKEYADASGRRVSIEYALIKDINDQPWRADLLGKKLHGALGAKVHVNLIPLNPTPGSKWDASPKRVQDEFQRRVIARGVTCTVRDTRGQEIAAACGQLAAEERSGAA",
    "response#3": "SMPPKHFADYTKPERIEILKELGLPKFRDNQIARQYYGRFQADPKKMTDLPESVRERIAETLFPPLMEPVRETSADDGNTQKMLWRLHDGTLLESVLMEYPDRATLCISSQAGCGMACPFCATGQGGLDRNLSTGEIVDQVRAAAATMQAKGGRLSNIVFMGMGEPLANYNRVLSAIHQITQPSPEGFGISQRGITLSTVGLAPAIRRFAKEGLSCRLAVSLHTPDDELRDSLVPANNRWPVEEVLDAAKEYADASGRRVSIEYALIKDINDQPWRADLLGKKLHGALGAKVHVNLIPLNPTPGSKWDASPKRVQDEFQRRVIARGVTCTVRDTRGQEIAAACGQLAAEERSGVR"
  },
  {
    "reference": "MSNRSITLLQRVDNATEQLNSKFTDIVNSAKVSSKDKSALAMETYLVEESTSAMVRSLEDLLFVTRSLKEAWILGQIRPVVNKPEDGGEGQLADELLDKIEDTSDGVDKETA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must be optimized for transcription coregulator activity.2. The protein must exhibit the following characteristics: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. The designed protein sequence is ",
    "response#1": "SCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSANGTECYITSDMFYVEVHLDPTGQLCDVKVAHHGENPVSCPELVQHLRERNFDEFSKHLKGLVNLYKLPGDSKLKTKMYLALQSLELDLTKMAVMYWQATNASPLDKILHGSVGYLTPRSGGHLINLKYYVSPYDLIEDGTGVPVILNEGGVPRNLGMNVCVTIEGTLTMNKLPIAPLIMGSHPVDNKGTPSFSSITSANSVDLPACFFLKFPRPIPVSRGFIQKLQNCTGIPLFDTPPTYVPLYELITQFELSKEQDAGSLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLLSKIAFHHPSRVPLILNMIRHQVAYNTLIGSCVI",
    "response#2": "SCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSANGTECYITSDMFYVEVHLDPTGQLCDVKVAHHGENPVSCPELVQHLRERNFDEFSKHLKGLVNLYKLPGDSKLKTKMYLALQSLELDLTKMAVMYWQATNASPLDKILHGSVGYLTPRSGGHLINLKYYVSPYDLIEDGTGVPVILNEGGVPRNLGMNVCVTIEGTLTMNKLPIAPLIMGSHPVDNKGTPSFSSITSANSVDLPACFFLKFPRPIPVSRGFIQKLQNCTGIPLFDTPPTYVPLYELITQFELSKEQDAGSLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLLSKIAFHHPSRVPLILNMIRHQVAYNTLIGSCVV",
    "response#3": "SCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSANGTECYITSDMFYVEVHLDPTGQLCDVKVAHHGENPVSCPELVQHLRERNFDEFSKHLKGLVNLYKLPGDSKLKTKMYLALQSLELDLTKMAVMYWQATNASPLDKILHGSVGYLTPRSGGHLINLKYYVSPYDLIEDGTGVPVILNEGGVPRNLGMNVCVTIEGTLTMNKLPIAPLIMGSHPVDNKGTPSFSSITSANSVDLPACFFLKFPRPIPVSRGFIQKLQNCTGIPLFDTPPTYVPLYELITQFELSKEQDAGSLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLLSKIAFHHPSRVPLILNMIRHQVAYNTLIGSCVI"
  },
  {
    "reference": "PSGYAVREAGPPAYYRPNSDNRRQGGRERLASTSDKGSMAVESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCY",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein with Nuclear receptor capability for targeted gene regulation.2. A protein that contributes to positive regulation of DNA-templated transcription, negative regulation of I-kappaB kinase/NF-kappaB signaling, positive regulation of phospholipase C activity, intracellular steroid hormone receptor signaling pathway by functioning in nucleus with DNA-binding transcription factor activity, nuclear estrogen receptor activity, steroid binding, sequence-specific DNA binding.3. The protein must exhibit the following characteristics: Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE- independent signaling; Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 .; Also mediates membrane-initiated estrogen signaling involving various kinase cascades; Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP; Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components; Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences; Nuclear hormone receptor. The designed protein sequence is ",
    "response#1": "MSGLGENLDPLASDSRKRKLPCDTPGQGLTCSGEKRRREQESKYIEELAELISANLSDIDNFNVKPDKCAILKETVRQMYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMRPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATFLEACKKENQNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMEKTMLQKSKRLRKSCQACRKGHKKLLQLLTCSSDDRGHSSLTNSPLDSSCKESSVSVTSPSGVSSSTSGGVSSTSNMHGSLLQEKHRILHKLLQNGNSPAEVAKITALLEACKKENQKGHKKLLQLLTCSSDDRGHSSLTNSPLDSSCKESSVSVTSPSGVSSSTSGGVSSTSNMHGSLLQEKHRILHKLLQNGNSPAEVAKITALLEARKKENQCAVCNDYASGYHYGVWYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCRTRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIR",
    "response#2": "MSGLGENLDPLASDSRKRKLPCDTPGQGLTCSGEKRRREQESKYIEELAELISANLSDIDNFNVKPDKCAILKETVRQKQEQLSPKKKENNALLRYLLDRDDPSDALSKELQPQVEGVDNKMSQCTSSTIPSSSQEKDPKIKTETSEEGSGDLDNLDAILGDLTSV",
    "response#3": "MSGLGENLDPLASDSRKRKLPCDTPGQGLTCSGEKRRREQESKYIEELAELISANLSDIDNFNVKPDKCAILKETVRQAHFCAVCSDYASGYHYGVWSCEGCKAFFKRSIQGHNDYICPATNQCTIDKNRRKSCQACRLRKCYEVGMVKRKQEQLSPKKKENNALLRYLLDRDDPSDALSKELQPQVEGVDNKMSQCTSSTIPSSSQEKDPKIKTETSEEGSGDLDNLDAILGDLTSTRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKCKQEQLSPKKKENNALLRYLLDRDDPSDALSKELQPQVEGVDNKMSQCTSSTIPSSSQEKDPKIKTETSEEGSGDLDNLDAILGDLTSSQES"
  },
  {
    "reference": "MDQKLSELVEVLTTSGEPQLNPEKLKELKKICRSSDEHINHVYHLLMTQLNQEHAEIRLSAFQIVTELFARSHLFRTLLISNFQEFLELTVETDHEQPLPPPKEVAQKMKILAIKTVQEWHEKFGEAYKKLSLGYHFLKQNKKIDFQDVRSRTQAERKREEEKQRRLENIYKEKVKKATAEMEDMLEEIQSSLTEMENCFRLLLPDPREFAVFTDEKDFASDMRTKPTSQSPSHSKSTSQSSAYSKSTSQVSFDNDDEQPCCSKNLPPFPSSCTSSASGAERSLGEGAKESDKSARKSDTDDSDGDYEGSREAFLRDHGLGSHAYSLSLEISTDLKVNENENNTDVLNNLMDAHKLLKQKYWPAVQSWIQLFTKAGTNSESLKCAIDVKKEIEAALKKYKEMNIDCHTKERKVMTASDDDDDDDEFEEVPEKEGYEPHIPDHLREEYGLEPSASKQPGKKTEVKRPNVPQVPPSQKRINDELNPTCAAATMKTMKDKMAKALPGSSRNAGEPKSKCPKRETDLSQAPVAPCGLDLHHWGEEQPSAGKMLKFSSLHRFWAPNEVDEEVESKELEALVKTRYVTLPGKFEPVKHKCLAPMPNGSLCERQDRYKCPFHGKIVPRDAIGVPINAEDRAREAREKFEKQGEEQDWRDPELMREIEQATGVDLGSSKCPVKGKGKGVKRNLKKKYPNLTDLKQKANTSRSRLEKKVFNTGSVKRVISAMNQADKRRHEKFANQFNYALN",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein's functionality will benefit from Polar residues, Basic and acidic residues compositional bias in its structure.2. Ensure the presence of a signal peptide within the protein.3. The protein should be designed to localize specifically to chromosome in order to perform its role in transcription-coupled nucleotide-excision repair, protein ubiquitination, response to UV more effectively.4. The protein must exhibit the following characteristics: Factor involved in transcription-coupled nucleotide excision repair (TC-NER), a mechanism that rapidly removes RNA polymerase II- blocking lesions from the transcribed strand of active genes. The designed protein sequence is ",
    "response#1": "MFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKR",
    "response#2": "MFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKS",
    "response#3": "MFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKQ"
  },
  {
    "reference": "MTAGPPEEFPIKQGSLLIRGAQTERGPVDVCISREGFIIAAGPDAGRTFRGEADSIIEGKGRVLLPGLVNTHTHAAMTLLRGYADDMPLQQWLSEKIWPLESHLTGDDVYWGTRLACLEMIKTGTTAFHDMYFFMEDAARAVEAAGIRAVLTYGFIDLFSEERREQEIRATERFVRHIKALENPQIKASVGPHAVYTVSQEGLRWLAEYSREEGIGIHIHLSETEQEVGDAVKAWGMRPPGILDRCGILTPRTVAAHCCWLDQAECTLLGERGVSVAYNPASNMKLAVNRAMPYHWLREAGVNVSLGTDGCSSNNNLDMFEEMKTGALLQKFYWNSPTLLPATEMLAMATSGGARALGIGSGTITPGTPADLILLDRHAPCNTPMHQIPSNAVYSCNGSAVTTVICQGRVLMLDRHIPGEEEVISRASAVAADLVKRGGTA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The substrate binding site should be located in a region of the protein that is accessible to the ligand.2. The protein have enhanced adenosine + H(+) + H2O = inosine + NH4(+).3. The protein must bind to Zn(2+) in order to perform its enzymatic function.4. For general function, the protein need meet that Likely functions in a S-adenosyl-L-methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions5. The designed protein must be optimized for 5'-deoxyadenosine deaminase activity. The designed protein sequence is ",
    "response#1": "LVIPGLINTHTHLSMTLFRGIADDLQLHKWLENYIWPLEKHLNEDYCYAGALLGCIEMIKSGTTAFNDMYFYMDSVAKAIEKCGLRAVISHGMIDLNNEDKMKKEIKESKRIVKKCHGMADGRISVALGPHSPYTCSEKLLKKTSKYANKNNLKIHIHVSETREEIKTIKERYGMRPFEYLDDIGFLGENVIAAHAVWLSEEEIKIIKNNNVKISHNPVSNMKLASGISPVSKMLDENITVSLGTDGAASNNNLDMLEEMKIAALLQKVHYLDPTKLSAKDVFAMATINGAKTLGINAGLIEVGKKADLVLINVKRCNLTPFRNPISHLVYSANGYNVTTVIVDGKIAYFREGEKVGETAGE",
    "response#2": "IPGLSNNHTHIPMTLLRGIADDMILQNWLREKIWPNEAKLTGEDVYYGALLGCLEMLRFGVTSFNDMYFFPEEIVRATKEVGIKGSIGFPIIDFGTPQCKDLDKLLSNCEKFIREYIEEKKIKPVVAPHAPYTCSKDTYIRCKEISQEYNVPIHTHLSETRYEVVEMENKMGMRPVEYLESIGILGDNLITAHCVWVTKEEVKRLGKYKVKVVHCPGSNMKLASGGVMPLVEMLREGVSLSLGTDGPASNNNLDMLEEMKICALLHKAHRWDPRVGDVDTVLRMSPAFANTHGHAAMALLRGYADDMHLQEWLSEKIWPLEAHLTGDDVYWGTKLACIEMIRSGTVAFNDMYFFMDMAAKAVDEMGIKGVLSYGFIDFGSEEKRESEIKATEALYKHIADMKNPRIKAAVGPHAPYTVSKEGLKWCAEFSKEKNIMLHTHLCETEKEVKDCIEANGVRPAKLLDDCGCLSERTVAAHCCWLDDADCELLGRRGCAVSHNPCSNMKLAVNRAMPYQKLIDSGVNVTLGTDGCSSNNNLDILEEMKTAALLQKFYWNSDTVLPSHEAVKMATSAGMKALGFGGGSLKVGNPADIVLFRTNISCMTPLYNTQSNIVYSAGTNAVDTVFCNGRECTAEELIRRAIPGFINCHTHLAMTLLRGYADDMELMPWLKEKIWPLEARLTEDDIRWGVKLGCLELIRFGITCYNDMYYFPDVTAEATREMGLRAFISGVVFDMKPEMLRDVEPFIRRWKGDELIMPAVGPHAAYTCSEETLLKVKEIADRHDAMVHMHVSETREEVDGFLLTKGMSPVEYLDHLGLLNERLAAVHCVWLSEEDCYLLAERKANVVDCTVSNLKLTSGIAPLNTLMKAGVNVCLGTDGASSNNNLSLFEEMKVTAIVQKNAYHTPAAFSAEQIWQLATENAYRAARLNMGLRAGALADLALIDLRRPWLCPETNMVSHLVYSMAGGVDTTIVNGR",
    "response#3": "IPGLSNNHTHIPMTLLRGIADDMILQNWLREKIWPNEAKLTGEDVYYGALLGCLEMLRFGVTSFNDMYFFPEEIVRATKEVGIKGSIGFPIIDFGTPQCKDLDKLLSNCEKFIREYIEEKKIKPVVAPHAPYTCSKDTYIRCKEISQEYNVPIHTHLSETRYEVVEMENKMGMRPVEYLESIGILGDNLITAHCVWVTKEEVKRLGKYKVKVVHCPGSNMKLASGGVMPLVEMLREGVSLSLGTDGPASNNNLDMLEEMKICALLHKAHRWDPRVGDVDTVLRME"
  },
  {
    "reference": "MNIKNFFEKYNIKINDQQIYKEALTHNSYANERKLKYSYQRLEFLGDAILQMYVSKFLFFHYSKLGEGELTRLRSSTVREESLSRIAKDINLGQLIRLGHGELMTKGYEKQSILADIFEALTAAVYIDQNEDGLLIWLEQTLFKYMKDPTFINVTKDFKSELQELLQSEKRSDLKYIIENEEFFVNENKTLYTVSVNLDGQKFGVGKGYSKQEAEQNAASDCLSKLKKPLTN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that can perform rRNA binding in cytoplasm to support rRNA processing, tRNA processing.2. For general function, the protein need meet that Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S); Digests double-stranded RNA; Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.3. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.4. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+) binding.5. The protein contains novel DRBM domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "NRAMVEGLLVRLPRGAMLLAAKENTPVAAPNAFYSELIAGQVAYVRPGSLNSGNLQALDKSLSGFAAKKVNALILDLRASEATGDLAQAAEFAKRFCPRGKTIFNLRKPAGRQDRVFNSDRDPAFRGVIMVLADGDTAGTAEAIAAALRFHNKALVIGQPTAGRAADYSDFPLPNGKILRVAVAEMIAPDGRSLFPEGVKPDLPVL",
    "response#2": "MDGVDELLLRKFNLTLPPGLLRTAFVHKSFSFENGACQNNERLEFLGDAVLGLVVSHYLFETCPEYTEGQLSAARSYIVSGTSIAQIAKELNLGQFMLLGKGEKLAGGMDKTSLLADLLESLIGAVMVDQYG",
    "response#3": "NRAMVEGLLVRLPRGAMLLAAKENTPVAAPNAFYSELIAGQVAYVRPGSLNSGNLQALDKSLSGFAAKKVNALILDLRASEATGDLAQAAEFAKRFCPRGKTIFNLRKPAGRQDRVFNSDRDPAFRGVIMVLADGDTAGTAEAIAAALRFHNKALVIGQPTAGRAADYSDFPLPNGKILRVAVAEMIAPDGRSLFPEGVKPDLPVY"
  },
  {
    "reference": "MSQAISALQKARLAYSPKLPDVFKGEFAIEYGETVLPVATHQDEIKMLFPGSYGKPMARIVAGKSNLPTDAITVGVVLSGGPAPGGHNAIAGLFDALKKANPENRLVGFLNGPKGVFTGKYIELTKEVVDSYRNTGGFDMIGSGRDKIESPEQLNGCEKTLKALKAVALVIIGGDDSNTNASILAEHFIARKLPVQVIGLPKTIDGDMRNEFIEASFGFDTASKVYAELVGNICRDSMSGRKYYHFVKLMGRAASHVALEVALQTQPNYAIISEEVQEKKLTLSKVVDEISDMIVRRAEAGKNYGVILIPEGIVEFLSDFGAMLEDIGHALGQYESDFDGMTDVAERSDLLASKLRADNAAVYKGLPFNVQVVLTVRDPHGNLLVSQIESERVIIEAVEQRLDKMKKDGKYNGKFAALAHFFGYEGRCVQPSNFDADYCYALGYTGAQLIRAGLTGYTVSSMNLAAPVSQWQMGGVPVTSMLTLEARKGNLKPVIRKALVELDGAPFEAFQKARETWKTEDAYLFPGPIQYFGPSDVCDAISMTLALESTKR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.2. The protein must be able to ATP binding.3. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate binding.4. The protein must exhibit the following characteristics: Consistently, PPi-PFK can replace the enzymes of both the forward (ATP- PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.. The designed protein sequence is ",
    "response#1": "RVGVLTGGGDCPGLNAVIRAAVRKGVQQYHYEFTGFKDGWRGLLTGAAVRLDIPAVRGILPRGGTMLGSSRTNPFKERDGVRRIRENLAKDEVDALIAIGGEDTLGVAARLWEQYGIPCVGVPKTIDNDLSATDYTFGFDTAVGIATEAIDRLHTTAESHMRVLVVEVMGRHAGWIALHSGLAGGANVILIPEQRFDLDEVCGWVTSRFRASYAPIVVVAEGAMPQDGDLVLKDAAAGLDSFGHVRLSGVGEWLAKEIERRTGKEARTTVLGHVQRGGTPSAFDRWLATRFGLHAIDAVHDGAWGTMVALQGSQITRVPLSEATAQLKTVDPELYAEAEVFFG",
    "response#2": "RVGVLTGGGDCPGLNAVIRAAVRKGVQQYHYEFTGFKDGWRGLLTGAAVRLDIPAVRGILPRGGTMLGSSRTNPFKERDGVRRIRENLAKDEVDALIAIGGEDTLGVAARLWEQYGIPCVGVPKTIDNDLSATDYTFGFDTAVGIATEAIDRLHTTAESHMRVLVVEVMGRHAGWIALHSGLAGGANVILIPEQRFDLDEVCGWVTSRFRASYAPIVVVAEGAMPQDGDLVLKDAAAGLDSFGHVRLSGVGEWLAKEIERRTGKEARTTVLGHVQRGGTPSAFDRWLATRFGLHAIDAVNDGAWGTMVALRGTDIVRVPLEAATGVLKTVPPERFAEAEVFFG",
    "response#3": "RVGVLTGGGDCPGLNAVIRAIVRRGVRDHGFEFVGYRDGWKGPLDDLTMPLGIEQCRGILPRGGTILGSSRTNPLKVDGGVDRIRENLVRDGVDALVVIGGEDTLGVATHLADLGVAVVGVPKTIDNDLSGTDFTFGFDTAVNIATEAIDRLHTTAESHHRVLVVEVMGRHAGWIALHAGMAGGASAVLIPEQPFDIEAICAHVETRFQTQYAPIIVVSEGAVPVEGGAMTVHPGEVDAFGHVRLGGIGDRLAVEIERRTGKEARTVVLGHIQRGGTPTAFDRWLATRFGLQAVDAVREGAWGTMVALRADTVVRVPLAEATAELKTVPAERYEEATRIFG"
  },
  {
    "reference": "MRVKGTKRNYWWSWGMMFLGMLMSCNATQLWVTVYYGVPVWKEASTTLFCASDAKAVDPEKHNVWATHACVPTDPNPLEVVLVNVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNVTIPANATVNTTASSTASSNNSSVLKMKDEMRNCSFNVTTGIGNKMRKDYALFYILDITPIESDNTSTSYKNYMLINCNTSVIKQACPKVSFEPIPIHYCAPAGFALLKCNDKNFNGTGWCRNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVVVKSENFSDNAKTIIVQLKKSVEINCTRPGNNTRESIHMGPGRAFYTTKVIGDIRQAYCNLSREGWEETLNQVVTKLGEQVKEDKIAFNQSSGGDPEIIMHSFNCGGEFFYCNTSKLFDSTWARNTSTGNNATWNKTGDSTNDTHIILPCRIKQIINMWQEVGKAMYAPPISGKISCLSNITGLLLIRDGGENRTNNETETFRPGGGNMKDNWRSELYKYKVVRIEPLGVAPTKAKRRVVQREKRAVGTLGAMFLGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARLLAVERYLKDQQLLGIWGCSGKLICTTTVPWNASWSNRSLDYIWNNMTWMQWEKEIDNYTGLIYTLIEGAQIQQEKNEQDLLQLETWASLWNWFDISNWLWYIKIFIMIVGGFIGLRIVFAVLSVVNKVRQGYSPLSFQTRFPAQRGPDRPDGIEGEGGEQDRGRSIRLVDGF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have at least one Retroviral envelope protein GP41-like, Human immunodeficiency virus 1 envelope glycoprotein Gp120 domain.2. The protein must exhibit the following characteristics: During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; Transmembrane protein gp41: Acts as a class I viral fusion protein; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm..3. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.4. The designed protein should have structural molecule activity within plasma membrane, host cell plasma membrane, virion membrane, host cell endosome membrane to regulate actin filament reorganization, clathrin-dependent endocytosis of virus by host cell, fusion of virus membrane with host plasma membrane, viral protein processing, mitigation of host antiviral defense response.5. The designed protein should contain at least one Di-leucine internalization motif motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "MRVMETRKNYQHWWRGGILLLGILMICNATKLWVTVYYGVPVWEDADAILFCASDAKAYIAESHNIWATHACVPTDPDPQEIPLENVTESFNMWKNNMVEQMHEDIISLWDESLKPCVKLTPLCVTLNCTNVTNNITXAYNITDKEELKNCSFNTTTEIRDKKRQEYAIFYKLDIVPINKNSNENSSSDYRLINCNVSTIKQACPKVSFDPIPIHYCAPAGFAILKCNDNMFNGTGECTNVSTVQCTHGIRPVVSTQLLLNGSLAEKDIVIRSENITDNAKIIIAHLNESIEITCTRPNNNTRKSIHLGPGQAFYATGDIIGDIRQAHCNISRTKWNEIIEKVREKLEKSFNNKTIIFNQSSGGDLEVTTHSFNCRGEFFYCNTSDLFNNSTGXNDTTITLPCRIKQIVRMWQKVGQAMYAPPIAGNITCRSNITGLLLMRDGGSDNSTNSTETFRPAGGDMRDNWRSELYKYKVVRIKPLGIAPTRARRRVVGREKRAVGLGAAFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLQDQKLLGLWGCSGKIICTTAVPWNSTWSSKSYDTIWNNLTWIKWEKEISNYTDLIYGILTESQNQQDRNEKDLLELDKWASLWNWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTLTHHQRDPDRPERIEEGGGEQD",
    "response#2": "MRVMGIRKNCQRLWRWGTMLLGMLMICSAADQLWVTVYYGVPVWKEANTTLFCASEAKAYSTEAHNVWATHACVPTDPNPQEVVLENVTENFNMWKNNMVEQMQEDVISLWDESLKPCAKLTPLCVTLNCTDVESNTTNSSSNTTAIPSGEIKNCSFYITTQIRNKVRKEHALFDRLDVVPIDNNSSTYMLINCNTSVITQACPKISFEPIPIHYCTPAGFALLKCNNKTFNGTGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEDIVIRSENFTNNAKTIIVQLNETVKIECIRPNNNTRRSIHLAGGRALYTTGEIIGDIRQAHCNVSKWNETLKRVAIKLKDQFKNKTIVFKPPSGGDPEIVMLTFNCGGEFFYCNTTKLFNHTWTNDNWSNSTEPDGHITLPCRIKQIINRWQEVGRAMYAPPIKGQINCTSNITGLLLTRDGGNTTNGSEIFRPGGGDMKDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRAVGFGAMFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGLWGCSGKLICTTNVPWNSSWSKKNLTDIWDNMTWMQWEREIDNYTDTIYRLLEVSQTQQEQNEKDLLALDSWKNLWSWFSISKWLWYIKIFIMIVGGLIGLRIIFAVISIVRRVRQGYSPLSFQTLLPAPRGPDRPEGIEEEGGERDRDRSVRLVNGFL",
    "response#3": "MRVKGIRKNYQHLWRWGTMLLGMLMICSAVDQLWVTVYYGVPVWKEANTTLFCASEAKAYSTEAHNVWATHACVPTDPNPQEVVLENVTENFNMWKNNMVEQMQEDVISLWDESLKPCAKLTPLCVTLNCTDVESNTTNSSSNTTALWVTVYYGVPVWKDADTTLFCASDAKAHETEVHNVWATHACVPTDPNPQEIYLKNVTEDFNMWKNNMVEQMQEDVISLWDQSLKPCVRLTHLCVTLNCTEAFKVANTTNVNATIAPTTSSTTVTGSTRPTATVPNLLKNITDEVKNCTFNMTTELRDKKQQAYALFYKLDLVPINKTLKTYRLINCNTSVIKQACPKVSFDPIPIHYCTPAGYAMLKCNDKNFTGIGTCNNVSSVQCTHGIKPVVSTQLLLNGSLAEKEIVIRSENLTGNTKTIIVHLNESVQINCTRPSENVRTGIHIGPGQVFYRTGDIIGDIRRAYCEINGTKWNETLKQVAKKLKKHFNKTIVFQPSSGGDLEITMHHFNCRGEFFYCNTSQLFNATCLSKENMEGCNSTINSTITLPCKIKQIINMWQGVGQAMYAPPISGVINCLSNITGILLTRDGGNTAVNETFRPGGGDMRDNWRSELYKYKVVEIKPLGIAPTRAKRRVVEREKRAVGIGAMFLGFLGAAGSTMGAASITLTVQVRQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARLLAVEXYLRDQQLLGIWGCSGKLICTTNVPWNASWSXKSYDDIWNNLTWVQWEREISNXTQQIYTLLEESQNQQEKNEQDLLSLDQWASLWNWFDISNWLWYIKIFIIIVGGLIXLRIVFAVFSIINRVRKGYSPLXFQT"
  },
  {
    "reference": "MSNERYNARESEPKWQAKWDEAKIFATRNDDPRPKYYVLEMFPYPSGRIHMGHVRNYTMGDVVARTMRARGHNVLHPMGWDAFGLPAENAAIERKVAPKAWTYDNIASMKKQLQTMGLSLDWAREFATCDPTYYKHQQKMFLDFLKVGLAERETRKLNWDPVDMTVLANEQVIDGRGWRSGAVVEQREMNQWVFKITRYAQDLLDALETLDRWPDKVRLMQRNWIGRSEGLLVRFALDAATTPAGETELKIFTTRPDTLFGAKFMAIAPDHPLAQAAAAKNEKIAAFVEECKKRGTAQAEIDTAEKQGIDTGIRALHPFDPTWQIPVYVANFVLMEYGTGAIFGCPAHDQRDLDFVNKYQLGNTPVVCPEGQDPLSFVITDEAYDGDGRLINSRFLDGMSIADAKEEVAKRLETATLGNAPVGERKVNFRLRDWGISRQRYWGCPIPVIHCEVCGVVPVPDKDLPVVLPEDVSFDKPGNALDHHPTWKHVPCPQCGGKAQRETDTMDTFVDSSWYFARFTDPWNETAPTTPDVVNRMLPVDQYIGGVEHAILHLLYSRFFTRAMKATGHLGLDEPFRGMFTQGMVVHETYRTADGHFASPAEVAIVAEGDGRRATLLDGGTPVEIGPIEKMSKSKRNTVDPDDIIGSYGADTARWFMLSDSPPDRDVIWSEEGVKGASRFVQRLWRMVNDAAAIARSAPAERPATFGPEALAVRKAAHGALDKVLSGIERLAFNVSLAHIREFANSFGEALAKPGQPSPDLAWAIREA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a low Km value for ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-   tRNA(Leu).2. Incorporating a 'HIGH' region, 'KMSKS' region motif is critical for the successful design of the protein.3. The protein should have a high affinity for ATP.4. The designed protein should be involved in leucyl-tRNA aminoacylation by localizing to cytoplasm and exhibiting leucine-tRNA ligase activity, aminoacyl-tRNA editing activity, ATP binding. The designed protein sequence is ",
    "response#1": "RNWIGKSEGLQVRFALAAGTAPAGFSEVEVYTTRPDTLFGAAFVAISADHPLAKKLSEGNAALSSFIEECHQQGTSLAALETAEKKGFDTGIKVKHPFDDNWELPVYVANFVLMEYGTGAVFGCPAHDQRDLDFANKYKLKVTPVVLPKGEDAASFSIGETAYTDDGVMINSRFLDGMTPEAAFNEVASRL",
    "response#2": "RNWIGRSEGLMVRFALDSATTPAGETELKIFTTRPDTLFGAKFMAIAADHPLAQAAAAKDPKVAAFIDDCKKRGTAQAEIDTAEKQGIDTGIRAMHPFDPSWKLPVYVANFVLMEYGTGAIFGCPAHDQRDLDFVNKYQLGNTPVVCPEGQDPASFVITDTAYDGDGRMINSRFLDGMTIVEAKEEVAKRLAS",
    "response#3": "RNWIGRSEGAEVTFGIDGTDENFTVFTTRPDTLFGATYCVLAPEHKLVEQITTVEQRQAVEDYLEKVKMKSDLERTDLAKEKTGVFTGAYAVNPINGKKVPIWIADYVLVSYGTGAIMAVPAHDERDYEFASAFSLDIVPVLEGGDISKEAFTGDGQHINSDFLNGLNKVEGIAKAIEWLE"
  },
  {
    "reference": "TSVTTTWENTGSTAPEYVSTEVTKADWKGTTSAEDQTGDGITGLSPTTSYTSFVTSETVTSPMAVEPSPISGLHVSFVGVTQATLTWENANGTAACRLLLEGIGSPKEWTQDLVVNISGLAPGTRHKVTLSLLEPNEQRESLVVNSVLGELQRCASCLPSLSPHHPLTRDTEVLLFGLKSNTQYEATVYSQAADGTEGQPRNVTFRTGTQVFDLKVVDISATNMTLSWKINDNESSSVYAYEIQVAGPTDSFTLRTNETGVIITSLNSSTLYNITVRPFLGNGSEGVSGFLQVYTPPGPVSDFRVTNVSTRAIGLAWWSNDSDFFKILTTENGTGKSQVNTTTDQSIVIGDLSPGTMYHFEIHPQGPNGTEGEPQRVCSSTDPSAVFDIRVLQVTTTEMELEWQNTDSSSDYTYTLDVQSKYGSNKTNSSHEAITLKDLVPGTLYNITIFPEVNHVVGESNFTAQYTLPSSVSDIEISTNTTAASLTWQNLDIASDTYTYHLLIEKDGNSSKATQLVTDMGVTSATVTELIPGSVYTVEIFTEVGNGTKSLAPSWQSFCTDPAPVTSFHCEVVPKEPTLILKWARPPGTNVGFRLEISRGAWRNVTDLGSCTSENGTEYRTEVTYLNYSTSYNISITASFCNKTASPAQNTCLTGITDPPSPDGSPNITSISHNSVKVEFSGFEASHGPIKAYALILTTGNAVYPSANVLRYTYEDFKKGASDTYVTYLITTEGKRRSQGLAEVLKYEVDVGNESTTHGYYNGKLEPL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The ideal protein would be one that plays a critical role in positive regulation of focal adhesion assembly, negative regulation of vascular permeability through beta-catenin binding within cell-cell junction. The designed protein sequence is ",
    "response#1": "GGPQGLVQIMRNYSYEKLLWTTSRVLKVLSVCPSNKPAIVEAGC",
    "response#2": "QMVAALVRAMTNTNDLETTRCAAGTLHNLSHHRQGLLTIFKSGM",
    "response#3": "QMVAALVRAMTNTNDLETTRCAAGTLHNLSHHRQGLLTIFKSGV"
  },
  {
    "reference": "MRESIQRRKNRLRIKRKRRVRGKITGSADRPRVSIFKSNRHFYAQAIDDTKGHTLAYSDGAKLGVKVNKEDVKKVAEDLAGKLKALNIETIVFDRNGYLYHGVVASFADALRENGIKF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that can perform structural constituent of ribosome, rRNA binding in ribonucleoprotein complex, ribosome, cytoplasm to support translation.2. The protein must exhibit the following characteristics: This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.. The designed protein sequence is ",
    "response#1": "MPPIGAKVTLRGADAQSFLEKALPLVTISERQFDETGNFSFGVEEHTTFPSQEYDPQIGIYGLDVTVNLTRPGYRVTKRDKASQPIPSV",
    "response#2": "PIGAKVTLRGADAQSFLEKALPLVTISERQFDETGNFSFGVEEHTTFPSQEYDPQIGIYGLDVTVNLTRPGYRVTKRDKASQPIPSV",
    "response#3": "PIGAKVTLRGADAQSFLEKALPLVTISERQFDETGNFSFGVEEHTTFPSQEYDPQIGIYGLDVTVNLTRPGYRVTKRDKASQPIPSR"
  },
  {
    "reference": "MRVLVFNAGSSSLKLGVFDGERQVFKASFDRFGPEGCDLKIGGETSRAPQPDLAGAIAAVPALLDRHGVEKIDAVGHRLAHGGTAFTGPVRIDDAVETRIEALTPLAPLHNPAMLQAVRLARQLWPDLPQVAVFDTSFHLTNPARATTYAVPKAWRDAGLRRFGFHGTSHRYVAQRAAEALGQDAKDLRIVSIHLGNGASACAVQYGRSIDSSMGMTPLEGLVMGTRSGDVDPGAFGFLQRELGLDIPQIEAALYADSGLKALTGSADMRDVEDRAAAGDLEAQLAINIYAYRARKYLGAYAAAMGGLDAVVFTGGIGENSASMRRRICDGLSFLGLMLDDDLNRAPDLTDRAAPQIQAQGSRVAVIVTETAEQLMIAREVRQLLARRAPVPRSIPVAVSGRHVHLSADAVDALFGPGYRLNPGAPLRQPGNWVAEERLSLEGPKGRLERVAILGPLRPRTQIEVSRTDSFALGLDAPVRDSGKLDGTPTVRLIGPAGQLDSNGLIVAARHIHTSPADAEAMGLTDGSLIRVRITGASGRDLVFDRVLIRVAPGTVTEMHIDTDEANAAGIRGAGEGELVGTAVAEL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Can also catalyze the reverse reaction.; Catalyzes the formation of acetyl phosphate from acetate and ATP.2. The protein design should consider the endogenous proteins and enzymes present in the Polyol metabolism pathway to avoid interference with native metabolic processes.3. The designed protein should have acetate kinase activity, acyltransferase activity, transferring groups other than amino-acyl groups, magnesium ion binding within bacterial microcompartment to regulate organic acid metabolic process, propanediol catabolic process.4. Zn(2+) is required for the proper folding and stability of the protein.5. The designed protein should have a high specificity for substrate, ATP binding, with minimal off-target effects.6. The design of the protein must focus on the precise arrangement of amino acids in the Proton donor/acceptor to enable effective catalysis of acetate + ATP = acetyl phosphate + ADP. The designed protein sequence is ",
    "response#1": "LFRGFDEGSLAELVANSTVAEAADGALLIEYGHAGEYLGVLLDGRATVRRPAPEPGAPPVEIGTLRPGDYFGEMSLLTGEVTTAEVAAVGPCRYLRIPQAVYARTLAANPVAVAELAKAMADGALLIEYGHAGEYLGVLLDGRATVRRPAPEPGAPPVEIGTLRPGDYFGEMSLLTGEVTTAEVAAVGPCRYLRIPQAVYARTLG",
    "response#2": "LFENFPADKVEEIVIKSRLATFEGNEAILETGDEGKFIGIMISGHAEISMSDNTGTRSVISQLQAGDVFGVMSLLTGDRIVADVIAETRCFVLMIPQAVFSTHILSSPKALGYLSRLLD",
    "response#3": "LFRGFDEGSLAELVANSTVAEAADGALLIEYGHAGEYLGVLLDGRATVRRPAPEPGAPPVEIGTLRPGDYFGEMSLLTGEVTTAEVAAVGPCRYLRIPQAVYARTLAANPVAVAELAKAMLFRGFDEGSLAELVANSTVAEAADGALLIEYGHAGEYLGVLLDGRATVRRPAPEPGAPPVEIGTLRPGDYFGEMSLLTGEVTTAEVAAVGPCRYLRIPQAVYARTL"
  },
  {
    "reference": "MKEYNIVITGVGGQGILTAANLLGWAALRAGYKVRVGEVHGMSQRFGSVIAYVRFGEDVYGAMVPEGKADVILSFEPVEALRYINYLKKGGLVFTNARPIPPVQVSMGLASYPSMEEIRKIVEEDFGGKFLAFDAEKLAIEAGNVITTNVVLIGALTQTPGFPLSAEHVKEVIRISVPPKAVDVNMRAFELGVKAAKEMLNL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates..2. The protein should be designed to have high stability and activity towards CoA + indole-3-pyruvate + 2 oxidized [2Fe-2S]-[ferredoxin] =   (indol-3-yl)acetyl-CoA + CO2 + H(+) + 2 reduced [2Fe-2S]-   [ferredoxin]. The designed protein sequence is ",
    "response#1": "DIGCYTLGVLPPLKTVDTTIAMGGSIGVAHGLSIALNGSIAEEQRKTGKGKKIIAATIGDSTFFHTGLPALANAIYNRSNVLIVVLDNLVTAMTGDQPNPGTGETPHGPGKRILIEEVAKAMGADFVAVVDPYDIKETYETFKKALEVEGVSVVV",
    "response#2": "GALEGNIGVYAAYPGTPSSEITDTMAAVASRAGVYMEYSTNEKVAFETALSASWAGLRAMTAMKHVGLNVAMDSFMTVSYMGVNGGLVVVVADDPSMWSSQNEQDTRAIAKFANIPVLEPSSVQEAKDMVKYAFEISEKYGQMVILRTTTRSSHMRGDVVLGELPQEIKEGKRKFGDFKKNPERYQ",
    "response#3": "GGQGIIKTSVIIGEAAMNEGMNVVMSEIHGMAQRGGAVSTEIRFGDVRGSIIPQGEADLVIAFEPLEALRALPKMSEDACVIVNTSKIPPFNLIKSPHPYPPLEEIIKTLEENAGRVRSFNGEKIAVEAGHILSLNMVMLGAAAATTGFPLGEETLIESMKNNLPPKLMEVNLRAFHEGFR"
  },
  {
    "reference": "MSLALSISGRRGAFQLQVDATLQPEGVTVLFGASGAGKSSLLRMVAGLDPCEGTIRFADQLWRSSGLSSERKNDVDLAVWQRPIGMMFQQPTLFHHLTVQGNLNYVARRRRSPIGQVEQVIKQTGIAPLLSRRVDGLSGGESQRVALARALLGTPRLLLLDEPLSALGEEHKQGLLDLIALAAQTVPVLYVTHNMDELLRLADQVWLMEQGRVVAQGPVGQELSRLDGVLAQRADATALLAGEVGSYVREDHLQGIRVGSHTLWLPGLQRLTPGEPVRLRIAARDVSLCLSAPNDSSILNILPAQVVGLRDVGPGQCLVQLRLAGQCILARISGRSARQLSLFPGTDLYAQIKAVALV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be optimized for plasma membrane-specific folding and stability, while maintaining ATP hydrolysis activity, ATP binding, ABC-type molybdate transporter activity.2. The designed protein must have at least one Mop, ABC transporter domain.3. The desired protein should be able to perform ATP + H2O + molybdate(out) = ADP + H(+) + molybdate(in) +   phosphate4. The protein must exhibit the following characteristics: Part of the ABC transporter complex ModABC involved in molybdenum import.5. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding. The designed protein sequence is ",
    "response#1": "MDGLRLRFRRAYPGFELDIDLALPGRGVTALFGHSGSGKSTCLRCIAGLEKAAEGEVTINGETWQDSRRNLFVAPHRRALGYVFQDANLFRHLTVRRNLAFGLKRIAAAERRVELEQACALLGIEHLLERMPERLSGGEQQRVGIARALLTSPRLLLMDEPLASLDLKRKGEILPYLERLHEELDIPVLYVSHSPDEVARLADHLVLLENGKVRASGPIGETLARVDLPLALDDL",
    "response#2": "MLNINIEKQLGQLQLKVNTQLPLQGVTAVFGRSGAGKTSLVNLLGGLTTPDKGEISLGDTLLFKHKTVNLPPEKRRIGYVFQEARLFPHYSVNGNLTYGMRHKTPELFDKVVSLLGIEKLLSRYPSTLSGGEKQRVAIGRALLTSPQMLLMDEPLASLDLPRKRELLPYLQTLAQELKLPIVYVSHSLDEILQLADHMLVLHQGKMIAQGPLTQVWNSEQMRPWVPLR",
    "response#3": "VLQHQTLNIAVQLDATKQIVGVLSNWKKQLGKNYVRLAPELENMAMPGDLNSVNIESLLALKPDVVFVTNYAPSEMIKQISDVNIPVVAISLRTGEVGEKGKLNPTLTDEDKAYNDGLKQGIELIAEVFEKKQQGDELVKAAFANRKLLADRLGDVSADKRVRTYMANPDLGTYGSGKYTGLMMEHAGAYNVAAATIKGFKQVSLENVLEWNPAVILVQDRYPDVVPQILNDQGWANIQALKDKKVFLMPEV"
  },
  {
    "reference": "MTTKTINCSRKIVIASHNAGKVCEITKLLEPLGLGVTSAAELDLPEPLENGQTFSANAIIKATAARDTTNLLSLADDSGLVVPTLNGAPGVYSARWAGPKKDYDVAMERIAKELAGREPAAHFVCALALAWPDGSCEHFEGTIHGTLVFPPRGINGFGYDPIFQPFGRLQTFGEMLPEEKHSISHRARAFEQFCNRYLGKSSGAE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein with metal ion binding functions is required to enable purine nucleoside triphosphate catabolic process, nucleotide metabolic process.2. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.3. The protein have enhanced dITP + H2O = dIMP + diphosphate + H(+).4. The designed protein should have a high specificity for Mg(2+), substrate binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MNTTEKLVIATRNKGKVVEIEAILKPYGVTVLGLSDFPEVPEVVEDGRTFEENALKKAREVARATGEWVLADDSGLEVDYLGGRPGVYSARYAGEGADDAANNAKLLHELADVPLEKRTARFRCVIAVASPGGDLLTTVEGVCEGRIGFAERGENGFGYDPVFYLPERGKTMAELSVEEKNALSHRGKALRALAERLKG",
    "response#2": "MSILSGKVALVTGSSSGIG",
    "response#3": "MTLNKYQD"
  },
  {
    "reference": "MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPLSGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVPFVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEFDISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRTDFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASFAFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYVMLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGLFVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNESGYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPRIICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGDYGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTTLDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTRYSEAGHPIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSGKPTSWQVNGESLLTREPKINFFKPMI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Two classes of point mutations dramatically improve activity of the enzyme.; The wild-type enzyme is an ineffective lactase The designed protein sequence is ",
    "response#1": "GVIAGSKGFIGAALMTSQGALRAGAGLVEVFVPEEIYPIVAAAAPQEAMVKPVKSYRDLLREKIDVWALGPGLGKARAVEILEVIEKAKQPMVIDADGLNILADKISILKRCRGKRLLTPHPGEMKRLFPSKKETRAATATKFCNRFAVTLLFKGSRTIVARRDRPLSYNTTGNPGMASGGMGDILTGVCAGLVGQGLSLYDAARLGAWICGRAAEIAIFNNTY",
    "response#2": "TYKNQCGRIGVIAGSKGFIGAALMTSQGALRAGAGLVEVFVPEEIYPIVAAAAPQEAMVKPVKSYRDLLREKIDVWALGPGLGKARAVEILEVIEKAKQPMVIDADGLNILADKISILKRCRGKRLLTPHPGEMKRLFPSKKETRAATATKFCNRFAVTLLFKGSRTIVARRDRPLSYNTTGNPGMASGGMGDILTGVCAGLVGQGLSLYDAARLGAWICGRAAEII",
    "response#3": "TYKNQCGRIGVIAGSKGFIGAALMTSQGALRAGAGLVEVFVPEEIYPIVAAAAPQEAMVKPVKSYRDLLREKIDVWALGPGLGKARAVEILEVIEKAKQPMVIDADGLNILADKISILKRCRGKRLLTPHPGEMKRLFPSKKETRAATATKFCNRFAVTLLFKGSRTIVARRDRPLSYNTTGNPGMASGGMGDILTGVCAGLVGQGLSLYDAARLGAWICGRAAEIL"
  },
  {
    "reference": "MNKYLFELPYERSEPGWTIRSYFDLMYNENRFLDAVENIVNKESYILDGIYCNFPDMNSYDESEHFEGVEFAVGYPPDEDDIVIVSEETCFEYVRLACEKYLQLHPEDTEKVNKLLSKIPS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The design objective is to achieve a protein with helix-helix-strand-strand-helix-strand-helix-strand-helix-helix-helix characteristics.2. The protein must exhibit the following characteristics: Binds the CT fragment of CdiA-STECO31, between two subdomains.; Protects cells against the tRNA endonuclease activity of cognate toxin CdiA-STECO31 but not other non-cognate toxins, inhibits the tRNase activity of the isolated CT fragment of CdiA-STECO31; Immunity protein component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. The designed protein sequence is ",
    "response#1": "TATSDLIESLISYSWDDWQVTRQEARRVIAAIRNDNVPDATIAALDKSGSLIKLFQRVGPPELARSLIASIAGRTTMQRYQARNALIRSLINNPLGTQTDNWIYFPTITFFDICADLL",
    "response#2": "KAEKVLKEFIIQMNQWELKYYPLFRNEGMTAYKDAAKKELDDIYDLFCTKKERKQGRQISLSCGEPPEYSPDEEVLSSELNKNKCVFITQQYTEAKNKFRYTLQFKEDEWRIDKKERFSFYDDKWIKYC",
    "response#3": "KAEKVLKEFIIQMNQWELKYYPLFRNEGMTAYKDAAKKELDDIYDLFCTKKERKQGRQISLSCGEPPEYSPDEEVLSSELNKNKCVFITQQYTEAKNKFRYTLQFKEDEWRIDKKERFSFYDDKWIKYL"
  },
  {
    "reference": "MKRAYSAASVRRVEAEYLSRGVPLMERAAHAIAHHALEMLTENHGAYGRRVLLLVGSGDNGGDALFAGGVMLSRGVQVEALALTSSVHESGGEEFVRRGGCWVNELTHSYDLVIDGFLGLGARAGISLEVLSALTEIQGVPMLAVDLPSGISADGEGMDQPALIPTVTVAIGSWKISHLLSESGLGVSELVDIGIDYESEIPALVSFEDSEVHELLPTEIETNHKYRRGVLGVIAGSETFPGAGILSIRAALALGIGMIRTESREFFSEVPEVVTTPGKIDALIVGPGLITLTQTQERATLEHLAKGGITLLDAGALAFLEKIPAEARQQIIITPHHGEFAAHFAPYAGALYANPLECAQNFVSAYGCHLLLKGPRTLIVSPGEIPVVNMRGSVALATAGSGDVLAGIIGNLMARTGAIRNSAIAGAYIHGKAGESLTAGASELIEILPFALR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP; Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration..2. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "MRELDRTTIEEVGIPGIVLMENAGRGAVETITSHFPNRVREGSRILIFCGKGNNGGDGFVIGRHLHNRGCQVRFLLFSPAEKVAGDARINLEAARRMGLAIVEVTDREALHKETEAIAGADLLIDALLGTGLTSELRGLYLAAVETMNQSATPVVAVDVPSGLDADRGHPLGTAVRAHLTVTFGLPKVGEIVYPGAEYTGKLVTVDIGIPRELLE",
    "response#2": "LLIAGSKGKTGAAILSAHATLRSGAGLVTVHLPEDATTPMQTALPEVMISYDRSKEAFSHLPDVSPYSAIAIGPGLGQRPETATALKLLIQESRVPLLFDADAINIISENKTWLSFLPPGCIFTPHLKEFERLTGATTDGFERLKMQQEFSVRYKCYVVLKGAFTSISTPEGITYFNPTGNPGMATGGSGDVLTGIILGLLTQHYTPLDAALLGVYLHGSAGDIAAGNLGVDTLIASDIIEPSGNKGTFGKVLVVAGSEGMCGAAYLSAAAALKAGAGMVQIQTVEANRIPLQILLPEAMVSCTFTEEENSRILSWCDVVVIGPGLGNYGLSYERELWFLKKAGELHKPVILDADGLNLLALRPKWKEYLGEHVVVTPHIGEMSRLTGKTIGEIQNCMAETALSYAEETKTTCVLKDACTVTAGREGSLYLNLSGNEGMATAGSGDVLTGILAAVFCMYLDGEASVSSEIKAALGVYLHGRCGDIAAEKYGSRSLTATNLIEALPDSYKNAHGHVLLIGGNRGMAGAIRLAAEAALRAGAGLVSVATHPDNITSVLQGRFELMVHGVESTNELSPLMKKASVIVLGPGLGQDAWAKALFNCAMVTELPMVVDADGLNCLAHAPRQKSNWVLTPHLGEARRLLSHSKTPLNEHDRFAVAKKISHEYGAVTVLKGPGSLISEGERININRSGCAGMASAGMGDVLSGIIGGLIAQGMEAFAATNLAVYIHGLAAEQAAHDGEKGMLAGDLFEHIL",
    "response#3": "VMIGGDRGTAGAIRLAAEAAVRSGAGLVRVITHADNIGPLLTARPELMVDELSEHTLADALEWADVIVIGPGLGQGDWGKQALKKAENTAKPMLWDADALNLLAIRPDKRQNRIITPHPGEAARLLNVSIGEIEGDRVAAAQRLAKRYGGVVVLKGAGTIIASQQAELAIADVGNPGMASGGMGDVLAGIIGALTGQKLSLYDAACAGCVVHGAAADRVAGLKGPRGMLASDLFSA"
  },
  {
    "reference": "MASSSLSTLCSSTSSSLHPNSKLSHSLSAKLSSKANVSVQFLGKKQSPLLSSTPRFLTVIAMAPPKPGGKAKKVVGVIKLALEAGKATPAPPVGPALGSKGVNIMAFCKDYNARTADKAGYIIPVEITVFDDKSFTFILKTPPASVLLLKAAGVEKGSKDPQQDKVGVITIDQLRTIAAEKLPDLNCTTIESAMRIIAGTAANMGIDIDPPILEPKKKAVLL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be optimized for stability and expression in large ribosomal subunit, cytosolic ribosome, chloroplast where it will participate in translation.2. For general function, the protein need meet that Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. The designed protein sequence is ",
    "response#1": "MAPGACMPLGKVPLGTTVHNVELKPGAGGQLVRSAGSSARVIARDGNYTLVTLPSGEKRLILSTCHATIGAVSNADRKNTKLGKAGRSRWLGRRPSVRGVAMNPVDHPMGGGEGKTSGGRHPVSPWGFK",
    "response#2": "MAPGACMPLGKVPLGTTVHNVELKPGAGGQLVRSAGSSARVIARDGNYTLVTLPSGEKRLILSTCHATIGAVSNADRKNTKLGKAGRSRWLGRRPSVRGVAMNPVDHPMGGGEGKTSGGRHPVSPWGFK",
    "response#3": "MAPGACMPLGKVPLGTTVHNVELKPGAGGQLVRSAGSSARVIARDGNYTLVTLPSGEKRLILSTCHATIGAVSNADRKNTKLGKAGRSRWLGRRPSVRGVAMNPVDHPMGGGEGKTSGGRHPVSPWGFG"
  },
  {
    "reference": "MSDRTSASSSSRRTKQNYDAIGSFVIDQEIGKGSFAKVYLGRHKVTGALVAVKSVELARLNKKLKENLYGEIQILKTLRHPHIVALHDCVESSTHINLIMEYCELGDLSLFIKKRDKLITNPYTHDLARKYPVYPNAGLNEVVTRHFLKQLASALQFLRAGDFVHRDVKPQNLLLLPSPHMMANNKTAKHIMSGSYDSFTPAAGLASAPMLKLADFGFARVLPSTSLAETLCGSPLYMAPEILRYEKYDAKADLWSVGTVLYEMVTGRPPFKASNHVELLRKIESSGDVIKFTRESVVSQEMKGLIRALLKKNPVERISFEDLFNHPVVTEPIPGLVEDDIPKPPRRRSLKEERPVSRAEDSLVPSRKQSLRKDLADREGAPQTAGPSSPKPRRSSPLATPNEPVEISKPNYFQIPPREDRLSYSPRKEAADGLGIKRPQVQPSTSAPTRPSSYVDRRHRSSNASLRAPVREANPPPNDVTRTKPRGMGTKPMTEEERAAQDIAFERDYVLVDKKHLEVNALADQISMYPQQPQSPKSAQIVRRATQQGSPTSTSGAVPSPPSRALQLAQGHSRQGSYDKALGTSPSKATSVISKAIQDASLRLFGFKYAPHLLSKGPSPVPMYNPFPTYPAPNTPTGLISDGKQGTPVDEDSRVAQCIEDHATRSDVVYGFAEVKYKQLVPLAPSMDHGLGGAPIEKSAEEDGLTAEAIVSLSEEALVLYVKALTLLAKSMDIASLWWSRKNKVESTNSITSAARDSANSEALALRI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have a specific function in response to starvation and should be localized to cytoplasm in order to carry out this function efficiently.2. The protein must exhibit the following characteristics: Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production; Also involved in endoplasmic reticulum-specific autophagic process, in selective removal of ER- associated degradation (ERAD) substrates. The designed protein sequence is ",
    "response#1": "TLTNQNDLQAWRNLYMARRSWALGLVERCGAMDKSIHEHNERTDIIHRAAGVALENLKTHVGNLENRFQEAQTWANDLLKEQRAALDGWQRALTTLESIPAPKVFPFLGRPSTPKEHRDRPTGTLRDFVDANEVQKAGAEAAAESSRFARQIDDVAEAVRGITADTQRLIDDQLPSGADAADGLQEMITFAKKISSDYEHVIALPNNQKTLANISRLALTHTQDLLPSMLDISAEIHAGLEEAVRRHNTAMKVALDHMRTISAIELRLADVQSQIINLNVQSDAFDVVFSVYHMPMVYGSILVESVRRREFNEKMKADSLTLAEEMAVFRDEEQRRRKKWLKSMGDFFV",
    "response#2": "TLTNQNDLQAWRNLYMARRSWALGLVERCGAMDKSIHEHNERTDIIHRAAGVALENLKTHVGNLENRFQEAQTWANDLLKEQRAALDGWQRALTTLESIPAPKVFPFLGRPSTPKEHRDRPTGTLRDFVDANEVQKAGAEAAAESSRFARQIDDVAEAVRGITADTQRLIDDQLPSGADAADGLQEMITFAKKISSDYEHVIALPNNQKTLANISRLALTHTQDLLPSMLDISAEIHAGLEEAVRRHNTAMKVALDHMRTISAIELRLADVQSQIINLNVQSDAFDVVFSVYHMPMVYGSILVESVRRREFNEKMKADSLTLAEEMAVFRDEEQRRRKKWLKSMGDFL",
    "response#3": "SLSSINHVWRANEIVTAARAALEECVVVSARTAFLRRGLNNQLRLLYSVRTEVEEISLRGRSEFAAVLKDLDDADTRLRRTLELLRETIVHPAFRPEGEDPKSLHDFVDERGVEELHAALKSSIDRTTAAQAQLDSSNHAFDDELLSIKEALGTYRTAAKLASSRSSSSASSSSTSNSSLPSISTMPSMLHSLEMHAQEMANLLESLVRHFDLCVTAVKHTEGGGAAAKSITGDMPVGVPVSGRMGSNIEEEINNNLNAPLDPLSNSEYQEMVNVLFKDAAEAEDVVMEIQDRIGEMESVLENVLAQRDALRSIYNATTDIYQHLSSLGSTRLPGYIAQAHNFTQVWHEENDRISGGLADLSDLNSLYNGFLDAYDGLIVEVARRKHVRQRVEKVLRDTRHKLDQLYEEDVTARETFRVEKGDYV"
  },
  {
    "reference": "EPFERSTRAARKNETLQETKTRFLLFEGATDRGCQIQLHRADTLQQCGFNASLPLVMIIHGWSVDGLLEDWIWQMVAALKSQLARPVNVALADWISLAQNHYAVAVRNTRLVGQEVAAILRWLEESAPFSRSNVHLIGYSLGAHVSGFAGSYIGGKHKIGRITGLDAAGPMFEGASPNDRLSPDDANFVDAIHTFTREHMGLSVGIKEPVAHYDFYPNGGSFQPGCHFLDLYKHITKHGLNAFTQTIKCAHERSVHLFIDSLEHPNLQSTAYLCSGMDSFSQGLCLDCKKGRCNTLGYHVRQGGQSKKSKQLFLVTRAQAPFKVYHYQFKIQFLNQIEKPVEPTFTMSLLGTDLEIQKIPITLGEGITSNKTYSFLITLDSDIGELLKIKFKWENSAVWANVWNTVQTIIPWGRGPLYSGLVLKNIRVKAGETQKRMVFCSESMEDLQLHPTQEKTFVRCDVNSKKMKRKNR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with phosphatidyl phospholipase B activity, lysophospholipase activity functions is required to enable triglyceride homeostasis. The designed protein sequence is ",
    "response#1": "SVHSLKPADVKIIAALGDSLTAGTGIASDNLLDLDTEYRGLSWSIGGDASLEDVTTLPNIFQEFNVTIVGYSTGTGSENDSNAFLNQAVPGAQAEHLPAQARNLVRLMKTDPRIDFSADWKLITVHIGGNDLCNCCKDPDHYSAVNFISRIRETLDILHEQASAVPKALVSLVEVMDLLPLRQLFMDTQVQCPTYMADYLCSCVLTGEENSPNLTMVREVTRDYQLGIRRLVESGRYDTHENFTVIIQPFLQNPKIPLGQDGHPDVSYFSPDCFHPSQKGHSQLAKALWNAVLQPVSPTLDYEYAKNEFAQAIELQYFKQGTVIVEQDTRGKGLYYVVSGKIDVTTSTVSDHEIFNSTRDKKKKKSKTLFTIESGGIAGYLSSLVSYKSFVTLIAKTDVYVGFLPYQTLEKLCDKYFLIYLRIAESLV",
    "response#2": "LVLGGGGARGLSHLGILQALEERGIPIDMIGGTSIGSFVGGLYAKDYDLVPIFGRIKKFAGRISSIWRMLSDFTWPVTSYTTGHEFNRGIWKSFGDTRIEDFWVQYFCNSTNITESVQEIHSYGYAWRYVRASMSLAGLLPPIEDNGSMLLDGGYVDNLPVLEML",
    "response#3": "SVHNLKPADIRVIAALGDSLTAGNGAASKPHDVADVLTQYRGVSWSVGGNENISTVTTLANILREFNPSLIGYSIGTGRETTDNAALNQAVAGNHAEHVPAQVRRLVDRMKNDTRINMQTDWKLITLLIGGNDLCDFCENPVHYSPENYTFNLQKALDMLHREVPRAFVNLVTILSITSLRELHKSANSCPKLLMRILCPCVLNPDDNSDEFKKLVYFNRRYQERTRQLVESGRYDTTDDFTVVMQPFLTNVNIPKTQEGLPDSSYFAPDCFHFSQKSHSQAARALWNNMLEPVDCPSNVTWIRNATTGLGSGERAYIEAREKLVQPVIEQMMAARGLETPPRTPNIGVALSGGGYRAMLTGLGGIMGMMNESTEASESETGGWLDGVSYWAGLSGGSWATGTFMSNGGQLPTNLLENLWNIDSNLVFPDDDKLSFYTELYTETNAKSDLGFPIQITDVWGLAIGSHVLPERYQLSNTPNLTFSSLPSVVSALGNASLPMPIIIAADRKRREAGELVIAENATVWEFTPYEFGSWAFGSQYKSPGAFTPIEYLGTSVDDGSPNGTCWKGFDQLSFVMGTSATLFNGAFLELNGTDSGLLTNLITAFLADLGEDQADISRIPNTFSNYNSGENPIYNLTYITLVDAGETNQNIPLEPLLVPTRDVDAIVAFDSSYDTDYIWPNGTALRTTYERAKVLAEHENTRVLMPEVPSMNGFVNGGYNSRPTFFGCNDTTTPLIIYVPSYPWSFAANTSTYQLSYENDEANEMLLNGMRSLTLNHSVPTWPTCFACALTDRSFMYTSENRSTTCQKCFDTWCWAGDD"
  },
  {
    "reference": "MFIEHPLIKPKTLEARLYQQIIAANALKKKTLCVLSTGLGKTAIAILVIAGILTKKDGKVLILAPSRPLVEQHYNRLKQVLNIDEDKIIALTGKIQPKKRAELYKKGKIFIATPQVIENDIIAGRINVDEFILLIADEAHHTTGDHAYAFVAKKFKDKCHILGLTASPGSDIDKVMEICENLGIEHVEVRTEDDEDVKPYIAKVKLIPIRIDLPNEFKRALKLINEALKERLKILKDAGVINSIADVTKTELIELNNKLFSYDEEVKYELIKVCSEALKLMHAKELLESQGKSVFLNYINKLSMQRTKSAKSIVNDEKVREAVNLLMKSDVEHPKLGKVVDMVKNILEKNKDERIIIFAQYRDTVEKIVNLLTQNGIKAIRFIGQANKEGKGMSQKEQIEAIERFKKEGSVLVSTSVSEEGIDIPSVNYIIFYEPVPSEIRFIQRRGRAMRGEGGKVYVLIAKGTADEAYYRSALYKEREMKRLLKNMCYLLNKRLQKKFEEKSKEEIKEETEEIKEKEIESKTAVKEETKEEEEKTKKPVTILDFIKQIEVKERSKSEEDKIKQEIKIPKKPIKIIVDVREKNMAKLLHNYANIELKTLEVGDYVLSDRVVVERKTAEDFVNSIIDKRLFSQLKNLKKVEKPLLIVEGENFSRLHENALKGAILSIILDFGIPIIFTKNAEETADLLIKIAEKEQIKEKRTVMVRYGKTAMSLKEQQKFIVESLPDVGGALAERLLKHFKTVENVFTAKEEELMKVEGVGKERAKKI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be optimized for ATP hydrolysis activity.2. Incorporating a DEAH box motif is critical for the successful design of the protein. The designed protein sequence is ",
    "response#1": "GFERPSAIQQRAIMPIVTGRDCIAQAQSGTGKTATFSVSILQRIDTTVKKTQALVLAPTRELAQQIQKVVIALGDYLNVDCHACVGGTAVREDIARLNEGPHIVVGTPGRVFDMINRGALKTEAVMMFCLDEADEMLSTGFKESIYEIFQLLPGETQVVLLSATMAPEVLDVTKKFMRDPIRILVKKDELTLEGIRQR",
    "response#2": "SLINLTVDTLNDGGQVLVFSSSRQGAVRIARKLAEYICSSPVRYIDPGEAGKLAEEVRETSSSRILAEELTGLIKCGVSFHHAGLELEVRRVIEEGFRRGVLRVLASTTTLAAGVNLPARRVIVNEYRRYEPGYGFIEIPVMEYKQMAGRAGRPGLDPYGEAIIIVSSKDEVDYVIDKYIKSPPEYVKSNFMNPTSLKFHTLA",
    "response#3": "YCSRTHSQLSQFSSELRRVKMPSSMPAELSTSDANTDEVQERVKHLTLGSRKNLCINPKVMSLGNAAAINERCLELQQPGIAAEKRCPYLPSKEDEGQILQFRDHTLATIKDIEDMGKLGKRMGICPYYASRSVLKHSENVLLKAQKIVTLPYPLLLQRSARDALDLSIKNHVVIIDEAHNLMDAICNIHSVTIRLSQC"
  },
  {
    "reference": "MKNILVTGGLGFIGSNFVNHISSKYDNVNIYVYDIGDYCASVENVEWNNRTKLIKGDIRNFDLIMHTLTEHEIDTIVHFAAHSHVDNSFKNSLAFTETNVFGTHVLLECSRMYGKLKLFFHMSTDEVYGEIDTTDTSREVSLLCPTNPYAATKAGAEHIVKSYFLSYKLPIIIARCNNVYGRNQYPEKLIPKFICSLLDGKKLHIQGTGNSRRNFIHAIDVADAVDLVINNGVIGETYNIGVTNEHSVLDVAQILCDIAGVNLENQLEYVPDRLFNDFRYNITNDKIKSLGWEQSRKDFKKELVELFDWYKVNRHRYNIPGSQ",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose   + H2O.2. The protein design should be able to enhance the efficiency of nucleotide-sugar metabolic process. The designed protein sequence is ",
    "response#1": "LGVDGGSTKTLAIVFDERSERIMGVGISGPSNFTNAPRETAESNISDAVRKACSEAGTDLDGIGIRVFGLAGIGDSREATELGKDIVRSIVGHADVYSDGLGAYKFANLNDDGVVFAPGTGSVGFIKNGSDPERFGGWGWFIGDEASASWMAKQAILFAEREHDGIAETGFLDVVRRYFGMDLYETVYAISKEKIAKRVVAALAPQVSAMARSGNRYAISIFEESSSYIADLLNAKSRIFGRSGKYSVLGGTMLAGDFYRDMIRKKSSVDVSIYYGYQVAIGDVLIE",
    "response#2": "LAFRFSNSLFYYNWNNKFIDHVQITVSETIGVEGRFNYFDTVGQIKDMVQNHLLQILTITTMSTPIDCHENSIRDEKVKILKSLRPFNINNIHKNVILGQYTSGIINQKKVKSYLDETNNQEYQMNKYTETFVSMKIYIDNDQWSGVPFYLRTGKRLPKKCSEIVIFFKTPPLNIFSKNYNTLSKNKLILSLQPNEAIKIYILNKKPKLTSQYKLDLITLDFNYSKFYKKIQLSDAYEKLLLESMKGIQSLFVRRDEVELAWKWIDSTLQCLHLKPRLPDLYPAGTWGPARSKTMINNDGYEWL",
    "response#3": "LGVDGGSTKTLAIVFDERSERIMGVGISGPSNFTNAPRETAESNISDAVRKACSEAGTDLDGIGIRVFGLAGIGDSREATELGKDIVRSIVGHADVYSDGLGAYKFANLNDDGVVFAPGTGSVGFIKNGSDPERFGGWGWFIGDEASASWMAKQAILFAEREHDGIAETGFLDVVRRYFGMDLYETVYAISKEKIAKRVVAALAPQVSAMARSGNRYAISIFEESSSYIADLLNAKSRIFGRSGKYSVLGGTMLAGDFYRDMIRKKSSVDVSIYYGYQVAIGDVLIE"
  },
  {
    "reference": "MDYNVKDFGALGDGVSDDTAAIQAAIDAAHAAGGGTVYLPAGEYRVSGGEEPSDGCLTIKSNVHIVGAGMGETVIKMVDGWTQNVTGMVRSAYGEETSNFGMSDLTLDGNRDNLSAKVDGWFNGYIPGQDGADRDVTLERVEIREMSGYGFDPHEQTINLTIRDSVAHDNSLDGFVADYQVGGVFENNVSYNNDRHGFNIVTSTNDFVLSNNVAYGNGGAGLVVQRGSYDLPHPYDILIDGGAYYDNALEGVQLKMAHDVTLQNAEIYGNGLYGVRVYGAQDVQILDNQIHDNSQNGAYAEVLLQSYDDTAGVSGNFYVTTGTWLEGNVISGSANSTYGIQERADGTDYSSLYANSIDGVQTGAVRLYGANSTVSSQSGSGQQATLEGSAGNDALSGTEAHETLLGQAGDDRLNGDAGNDILDGGAGRDNLTGGAGADTFRFSARTDSYRTDSASFNDLITDFDADEDSIDLSALGFTGLGDGYNGTLLLKTNAEGTRTYLKSYEADAQGRRFEIALDGNFTGLFNDNNLLFDAAPATGTEGSDNLLGTDAGETLLGYGGNDTLNGGAGDDILVGGAGRDSLTGGAGADVFRFDALSDSQRNYTTGDNQADRILDFDPTLDRIDVSALGFTGLGNGRNGTLAVVLNSAGDRTDLKSYDTDANGYSFELSLAGNYQGQLSAEQFVFATSQGGQMTIIEGTDGNDTLQGTEANERLLGLDGRDNLNGGAGDDILDGGAGRDTLTGGTGADTFLFSTRTDSYRTDSASFND",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be designed to catalyze the following reaction [(1->4)-beta-D-mannuronosyl](n) = [alginate](n).2. The designed protein must be able to perform calcium ion binding under specific conditions.3. Ideal protein should have a binding affinity for key enzymes in the Glycan biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "WTLSSRALNRTVPAQFPSQVHLDLLRAGVIGEYHGLNDFNLRWIAAANWTYTSQPIKGLLDNYDSTWLVFDGLDTFATISFCGQQIASTDNQFRQYAFDVSTALGSCKGDPVLSINFGSAPNIVDAIAQDSNSQKWPDDVQLTYEYPNRWFMRKEQSDFGWDWGPAFAPAGPWR",
    "response#2": "GALGDGVSDDTAAIQAAIDAAYAAGGGTVYLPAGEYRVSGGEEPSDGCLTIKSNVHIVGAGMGETVIKLVDGWDQDVTGIVRSAYGEETSNFGMSDLTLDGNRDNTSGKVDGWFNGYIPGEDGADRDVTLERVEIREMSGYGFDPHEQTINLTIRDSVAHDNGLDGFVADFQIGGVFENNVSYNNDRHGFNIQ",
    "response#3": "WTLSSRALNRTVPAQFPSQVHLDLLRAGVIGEYHGLNDFNLRWIAAANWTYTSQPIKGLLDNYDSTWLVFDGLDTFATISFCGQQIASTDNQFRQYAFDVSTALGSCKGDPVLSINFGSAPNIVDAIAQDSNSQKWPDDVQLTYEYPNRWFMRKEQSDFGWDWGPAFAPAGPWR"
  },
  {
    "reference": "MKKNENAHKKMWEGRFSEASSKLLEEFNASINFDKNLFEEDIAGSKAHAKMLGICGILKKDESEAIIKGLDEVLAEIRAGKFAFKIEDEDIHMAVEKRLSQIIGAELGGRLHTARSRNDQVALDFKFYVLKKNLEISSLIKELIATLTNLAKNHKDTLMPGYTHFQHAQPVSLSYHLLAYAFMFKRDFERFVSSYERNNLSPLGSAALAGTPHKIDRSIVASELGFADCTQNAMDSVSDRDYALEILFNISVFMTHASRLCEELILWSSQEFGFVSISDAYSTGSSIMPQKKNPDVAELIRGKTGRVNGNLVALLTTMKGLPLAYNKDMQEDKEGVFDSVATILSSATILNEMIKTAKFNEKNMLKATKTGHLSATDLADYLVREKNIPFRTAHFITGKAVAKAESLGLDLSELNKEQLKSVDENLDENAIKFLDLHASKEARTSKGGTANKSVEEQIQILDDWLK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that can modulate the overall flux of metabolites through the L-arginine biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "TPSSHTTFVKRGMPLTMIPNPRVEVWTAQNRPRLSLDDPSIDLPRLVQLIEDSFNRKLDVQDYLNRVNDRLAGLIIAGEYEGGAILTWELPPGVEDDGSPASEARMVPYLDKFAVLKRSQGAGGVADIVFNAMVRSCFPNGVCWRSRKDNPVNKWYFERSTGTWKLSDTNWTMFWTTPGLTENSQRFSDYEQVL",
    "response#2": "TPSSHTTFVKRGMPLTMIPNPRVEVWTAQNRPRLSLDDPSIDLPRLVQLIEDSFNRKLDVQDYLNRVNDRLAGLIIAGEYEGGAILTWELPPGVEDDGSPASEARMVPYLDKFAVLKRSQGAGGVADIVFNAMVRSCFPNGVCWRSRKDNPVNKWYFERSTGTWKLSDTNWTMFWTTPGLTENSQRFSDYEQVC",
    "response#3": "TPSSHTTFVKRGMPLTMIPNPRVEVWTAQNRPRLSLDDPSIDLPRLVQLIEDSFNRKLDVQDYLNRVNDRLAGLIIAGEYEGGAILTWELPPGVEDDGSPASEARMVPYLDKFAVLKRSQGAGGVADIVFNAMVRSCFPNGVCWRSRKDNPVNKWYFERSTGTWKLSDTNWTMFWTTPGLTENSQRFSDYEQVC"
  },
  {
    "reference": "MQKRIALSFPEEVLEHVFSFIQLDKDRNSVSLVCKSWYEIERWCRRKVFIGNCYAVSPATVIRRFPKVRSVELKGKPHFADFNLVPDGWGGYVYPWIEAMSSSYTWLEEIRLKRMVVTDDCLELIAKSFKNFKVLVLSSCEGFSTDGLAAIAATCRNLKELDLRESDVDDVSGHWLSHFPDTYTSLVSLNISCLASEVSFSALERLVTRCPNLKSLKLNRAVPLEKLATLLQRAPQLEELGTGGYTAEVRPDVYSGLSVALSGCKELRCLSGFWDAVPAYLPAVYSVCSRLTTLNLSYATVQSYDLVKLLCQCPKLQRLWVLDYIEDAGLEVLASTCKDLRELRVFPSEPFVMEPNVALTEQGLVSVSMGCPKLESVLYFCRQMTNAALITIARNRPNMTRFRLCIIEPKAPDYLTLEPLDIGFGAIVEHCKDLRRLSLSGLLTDKVFEYIGTYAKKMEMLSVAFAGDSDLGMHHVLSGCDSLRKLEIRDCPFGDKALLANASKLETMRSLWMSSCSVSFGACKLLGQKMPKLNVEVIDERGAPDSRPESCPVERVFIYRTVAGPRFDMPGFVWNMDQDSTMRFSRQIITTNGL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for (indol-3-yl)acetate, 1D-myo-inositol hexakisphosphate binding.2. A protein that contributes to response to auxin, lateral root formation, stamen development, ethylene-activated signaling pathway by functioning in SCF ubiquitin ligase complex, nucleus with inositol hexakisphosphate binding, auxin binding, auxin receptor activity, ubiquitin-protein transferase activity.3. helix-helix-helix-strand-helix-helix-strand-helix-helix-strand-helix-strand-strand-helix-strand is the desired arrangement for the engineered protein.4. For general function, the protein need meet that The SCF(TIR1) E3 ubiquitin ligase complex is involved in auxin-mediated signaling pathway that regulate root and hypocotyl growth, lateral root formation, cell elongation, and gravitropism; Appears to allow pericycle cells to overcome G2 arrest prior to lateral root development; Confers sensitivity to the virulent bacterial pathogen P.syringae.; Plays a role in ethylene signaling in roots5. The designed protein should interact with enzymes and substrates within the Protein modification, protein ubiquitination pathway to enhance or regulate its activity.6. The designed protein must have at least one F-box domain. The designed protein sequence is ",
    "response#1": "MDSSPVSARFDIFEIYRRFCAIRSGQQLCNKKPCDGEESQRFNLSKEAITQLLYLVENKFQARNSIFDELFKLMSRLDLMVDFTEFTCFYDFVFFMCRENGQKNITISRAITAWKLVLAGRFRLLNRWCDFIEKNQRHNISEDTWQQVLAFSRCVHENLEGYDSEGAWPVLIDDFVEHMSKGSDSASSGDEKRLMQIILNIVGNAVKFSKQGSISVTALVTKSDTRAADFFVVPTGSHFYLRVKVKDSGAGINPQDIPKIFTKFAQTQSLATRSSGGSGLGLAISKRFVNLMEGNIWIESDGLGKGCTAIFDVKPKEEGSGASGLEGLNEPAFDSEQYMMLYTTIYNMCTQKPPHDYSQQLYDKYREAFEEYINSTVLPALREKHDEFMLRELFKRWSNHKVMVRWLSRFFYYLDRYFIARRSLPPLNEVGLTCFRDLVYNELHSKVKQAVIALVDKEREGEQIDRALLKNVLDIYVEIGMGQMERYEEDFESFMLQDTSSYYSRKASSWIQEDSCPDYMLKSEECLKKERERVAHYLHSSSEPKLVEKVQHELLVVFASQLLEKEHSGCRALLRDDKVDDLSRMYRLYHKILRGLEPVANIFKQHVTAEGNALVQQAEDTATNQVANTASVQEQVLIRKVIELHDKYMVYVTECFQNHTLFHKALKEAFEIFCNKTVAGSSSAELLATFCDNILKKGGSEKLSDEAIEDTLEKVVKLLAYISDKDLFAEFYRKKLARRLLFDRSANDDHERSILTKLKQQCGGQFTSKMEGMVTDLTLARENQNSFEDYLGSNPAANPGIDLTVTVLTTGFWPSYKSFDINLPSEMIKCVEVFKGFYETKTKHRKLTWIYSLGTCHINGKFDQKAIELIVSTYQAAVLLLFNTTDKLSYTEILAQLNLSHEDLVRLLHSLSCAKYKILLKEPNTKTVSQNDAFEFNSK",
    "response#2": "MDSSPVSARFDIFEIYRRFCAIRSGQQLCNKKPCDGEESQRFNLSKEAITQLLYLVENKFQARNSIFDELFKLMSRLDLMVDFTEFTCFYDFVFFMCRENGQKNITISRAITAWKLVLAGRFRLLNRWCDFIEKNQRHNISEDTWQQVLAFSRCVHENLEGYDSEGAWPVLIDDFVEHMLDRHTILKTTLVELGRTLALEECALWMPTRTGLELQLSYTLRHQHPVEYTVPIQLPVINQVFGTSRAVKISPNSPVARLRPVSGKYMLGEVVAVRVPLLHLSNFQINDWPELSTKRYALMVLMLPSDSARQWHVHELELVEVVADQVAVADTLEELPLEGLNEPAFDSEQYMMLYTTIYNMCTQKPPHDYSQQLYDKYREAFEEYINSTVLPALREKHDEFMLRELFKRWSNHKVMVRWLSRFFYYLDRYFIARRSLPPLNEVGLTCFRDLVYNELHSKVKQAVIALVDKEREGEQIDRALLKNVLDIYVEIGMGQMERYEEDFESFMLQDTSSYYSRKASSWIQEDSCPDYMLKSEECLKKERERVAHYLHSSSEPKLVEKVQHELLVVFASQLLEKEHSGCRALLRDDKVDDLSRMYRLYHKILRGLEPVANIFKQHVTAEGNALVQQAEDTATNQVANTASVQEQVLIRKVIELHDKYMVYVTECFQNHTLFHKALKEAFEIFCNKTVAGSSSAELLATFCDNILKKGGSEKLSDEAIEDTLEKVVKLLAYISDKDLFAEFYRKKLARRLLFDRSANDDHERSILTKLKQQCGGQFTSKMEGMVTDLTLARENQNSFEDYLGSNPAANPGIDLTVTVLTTGFWPSYKSFDINLPSEMIKCVEVFKGFYETKTKHRKLTWIYSLGTCHINGKFDQKAIELIVSTYQAAVLLLFNTTDKLSYTEILAQLNLSHEDLVRLLHSLSCAKYKILLKEPNTKTVSQNDAFEFNSK",
    "response#3": "MDSSPVSARFDIFEIYRRFCAIRSGQQLCNKKPCDGEESQRFNLSKEAITQLLYLVENKFQARNSIFDELFKLMSRLDLMVDFTEFTCFYDFVFFMCRENGQKNITISRAITAWKLVLAGRFRLLNRWCDFIEKNQRHNISEDTWQQVLAFSRCVHENLEGYDSEGAWPVLIDDFVEHMFQEQQQPPWLVPAREGEPAPADRWHMFGQEDNADAFSLFLDRLSETENFIKDAGFKAQISSWLAQLAEDEALRANTFAMATEATSSCEDRVTFFLHQMKNVQLVHNAEKGQYDNDLAALVATGREMFRLGKLEQIAREKVRTLALVDEIEVWLAYQNKLKKSLGLTSVTSEMRFFDVSGVTVTDLQDAELQVKAAEKSEFREWILQWGPLHRVLERKAPES"
  },
  {
    "reference": "MLTGRNILLGISGGIAAYKTPQLVRLLKKSGAGVQVLATASALKFVSELSLATVSNHPVLTGIFPSHEAREDEYTRHIALGEWADAFVIAPATANTLARLATGLCDDMLSLCFLTLRPGKPVLIVPAMDGHMYDSPSVQRNLATLRAQGCRLMEPESGSLASGQCGLGRMPEPETIFEALTGMLAAPEASALCGKSVVVTAGPTREKIDGVRFLSNYSSGKMGFAIAANAARRGARVHLVTGPVNLPTPSGVERIDVESAVEMLEAAQPLFGECDLFIGAAAVADYRPEAPVEGKIKKNEAEMEIRLVRNPDILAEFATNRKLGQLAVGFALETAGGIDYARKKLADKKLDLVAFNVYDGRTSGFEVDTNALTLLARDGGVTELPLLPKEEAAARLLDAVESLLPR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The CTP binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must have high specificity towards phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity.3. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.4. The protein have enhanced H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.5. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..6. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domain. The designed protein sequence is ",
    "response#1": "MQIVLGVAGGIAAYKAVLLLRLLREAGHDVRVVPTRAALEFVGRPTWEALSGQSATPEVFDDVAHVPHVALGQHADLVIVAPATADLLARAAAGRADDLLTSTLLTARCPVLMAPAMHTEMWDHPATRANVATLRARGVHVLDPASGRLTGADTGPGRLPEPDQIAAAARLLLADDAPLADLTGLHVVVSAGGTREPIDPVRYLGNRSSGRQGLAVARAAHERGARVTLVAAHVEDRLLDTLPAGVEVVMVGTAVELGEAVRLAARDADVVVMAAAVADFRPASVSATKIKKHRPGDSGGAAGTGVLELVDNPDVLAGLVADPPRRDGRTLVVGFAAETGDGDGDVLVHGAAKARRKGADLLAVNAVGTERGFGDVPNSVVVLDAAGEVVAEATGSKREVADAVWDAVLRLRA",
    "response#2": "MRVLLGVGGGIAAYKSAILLRLLTESGHDVRVVPTAASLEFVGRATWEALSGKPATSEVFEDVPSVEHVRLGKTADLVVVAPATADLLARAATGRADDLLTAALLTARCPVLMAPAMHTEMWEHPATRENVATLRSRGVTVLDPAHGRLTGADTGPGRLPEPEDIASAALEIAALLAAPAGGLTGLHVVVSAGGTREPIDPVRYLGNRSSGRQGLAVARAAHERGARVTLVAAHVEDRLLDTLPAGVEVVMVGTAVELGEAVRLAARDADVVVMAAAVADFRPASVSATKIKKHRPGDSGGAAGTGVLELVDNPDVLAGLVADPPRRDGRTLVVGFAAETGDGDGDVLVHGAAKARRKGADLLAVNAVGTERGFGDVPNSVVVLGADGSQELAGRHVVISAGGTREPLDPVRYLGNRSSGRQGYALARTARDRGARVTVVAANVSLDDPAGVDVVEVETTDQLRTAVRAAARTADVVVMAAAVADFRPVAAAGHKVKKRSDGVVDPILLEANPDILAELTAERLRPGQVIVGFAAETGDEHTTVLEYGRAKARRKGADLLAVNAVGHGLAFGTTDNAVTIIGPDGSHPDVTGSKAAVADAVWDAVTALRGR",
    "response#3": "MQIVLGVAGGIAAYKAVLLLRLLREAGHDVRVVPTRAALEFVGRPTWEALSGQSATPEVFDDVAHVPHVALGQHADLVIVAPATADLLARAAAGRADDLLTSTLLTARCPVLMAPAMHTEMWDHPATRANVATLRARGVHVLDPASGRLTGADTGPGRLPEPDQIAAALALLLRDGAEPLTGLHVVVSAGGTREPIDPVRYLGNRSSGRQGLAVARAAHERGARVTLVAAHVEDRLLDTLPAGVEVVMVGTAVELGEAVRLAARDADVVVMAAAVADFRPASVSATKIKKHRPGDSGGAAGTGVLELVDNPDVLAGLVADPPRRDGRTLVVGFAAETGDGDGDVLVHGAAKARRKGADLLAVNAVGTERGFGDVPNSVVVLGADGSETAVPYGPKEALADVVWDLVVPRLG"
  },
  {
    "reference": "MIETLESTQVSHETLRTIDAYWRAANYLSVGQIYLSDNPLLKRPLRIEDVKKMLLGHWGTTPGQNFIYAHLNRTIKQNDLNMIYVSGPGHGGPAVVANTYLEGSYSEIYPHISQDEAGMRKLFVQFSFPGGIPSHASPECPGSIHEGGELGYSLSHSFGAVFDNPDLIVACVVGDGEAETGPLATAWHSNKFLNPATDGAVLPILHLNGFKIANPTILARINHEELEQLMRGYGWTPIFVEGKDPMKMHAAMAEALDVAIGQIRSIQQNARETGDTSRPRWPMIVLRSPKGWTGPKFVDGVRNEGTFHSHQVPLSDPAKCPEHLKQIEQWLRSYRPEELLDENGRLRQEIADLAPTGDRRMGANPHANGGRLLRRLKMPDFRDYAVEISQRGCRGIGDTHVTGKFIRDIVRLNEEHKNFRIFGPDETISNGLEAVFDVTQRQWNAAIVEDDESLAPTGRVLEMLSEHQCEGWLEGYLLTGRHGLFNCYEAFVHIVDSMFNQHAKWLKVTSELPWRHKIASLNYLLASHVWRQDHNGFTHQDPGFLDVVVNKKAEIVRVYLPPDANCLLSVMDHCLRSQHYVNVVVAGKHPSPQWLTMGEAAEHCAKGIGIWDWAGNESSSDPDVVMACCGDVPTLETLAAVSILREHLPDLTIRVVNVVDLMRLQPKSEHPHGLSDSDFDALFTKNKHVIFAFHAYPWLVHRLTYRRTNHANIHVRGYKEEGTITTPFDMTVLNDLDRFHLVMDAIDRLPETGGRGQRLKALMQEKLV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. thiamine diphosphate is required for the proper folding and stability of the protein.2. The designed protein should be optimized for aldehyde-lyase activity activity in order to improve carbohydrate metabolic process. The designed protein sequence is ",
    "response#1": "PTDEHGGDLVFFQGHASPGVYARAFLEGRISEEQLENFRQEVDGNGLSSYPHPWLMPDFWQFPTVSMGLGPIQAIYQARFMKYLESRGFIPAGKQKVWCFMGDGECDEPESLGAISLAGREKLDNLIFVINCNLQRLDGPVRGNAKIIQELEGVFRGAEWNVNKC",
    "response#2": "PTDEHGGDLVFFQGHASPGVYARAFLEGRISEEQLENFRQEVDGNGLSSYPHPWLMPDFWQFPTVSMGLGPIQAIYQARFMKYLESRGFIPAGKQKVWCFMGDGECDEPESLGAISLAGREKLDNLIFVINCNLQRLDGPVRGNAKIIQELEGVFRGAEWNVNKC",
    "response#3": "PTDEHGGDLVFFQGHASPGVYARAFLEGRISEEQLENFRQEVDGNGLSSYPHPWLMPDFWQFPTVSMGLGPIQAIYQARFMKYLESRGFIPAGKQKVWCFMGDGECDEPESLGAISLAGREKLDNLIFVINCNLQRLDGPVRGNAKIIQELEGVFRGAEWNVNKL"
  },
  {
    "reference": "MPKPATQNIIASLAQFGITLTNTLALDGFVGDLHNDSRLLVTGDIFCAIIGHQQDGRQYIEQALKQGVKLVIRECDSAAEHGELVISSNGNGVVIVNFYQLNQHLFTLTKHYYQAPQKHMTIVGITGTNGKTTTSQLLAQLFTHANKPCAVIGTNGAGSIDKLAPIENTTPGACQLHQLFNRFIDEHSVHGKFSHVAMEVSSHALSQQRVQADLFDIAVFTNLSRDHLDYHDSMASYGAAKKQIFTGDNRQVAVVNFDDEQAKNWLKNWPEQQVLWLYGRADNIECNQHFVTAKYIKHHSHGVSFTLTSHLGRVVISSPLLGDFNIDNLLAAICVLLIQGTPLSELPDLVRQVSAIAGRMEVTSANNLATAVVDYAHTPDALEKALQACRQHCSGKLYVVFGCGGDRDKGKRPLMAQAAEKYADCVVVTNDNPRSEDAQLIANDIIAGFTHPDADTITIILAREQAVLTTLKTAQAGDMVLLAGKGHEDYVIVAKYDEHGVIIGTEKLAYNERLVVANFYQQHAGLATLPNKAKI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be optimized for activity in peptidoglycan biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "AFILGGSMGSRAINRGTVEALRTLLRDNDISVVHATGLSTSGEYRAFEDTAERLQRAGLGSTVPGRYVCKPFFDNIQDVYALADLVVARAGAGTIMELATVGKPSLLIPKSDVPGDHQLMNAMSLLKTGSAQVLFEERGEEDGLPITRVRGDALAQKISLLMSDEKNLARMGRKARQIAVRV",
    "response#2": "VTGTNGKTSTAVFLRELWRSKGNKAASLGSLGVGGDGPISAASLTTPDPVTLHRTLLTLAEAEVEHCVIEASSHGLDQCRLDGVRVSASAFLNLTRDHLDYHGDDESYFAAKERLFSAVMKPGGTAVLNAKAPTFTRLKDVCKARRHNILTFAGAKSDIRLQSRQDEGFGQRITLDLLGKSYDLFIPLPGAFQIENMMAAAGLM",
    "response#3": "AFILGGSMGSRAINRGTVEALRTLLRDNDISVVHATGLSTSGEYRAFEDTAERLQRAGLGSTVPGRYVCKPFFDNIQDVYALADLVVARAGAGTIMELATVGKPSLLIPKSDVPGDHQLMNAMSLLKTGSAQVLFEERGEEDGLPITRVRGDALAQKISLLMSDEKNLARMGRKARQIAVRR"
  },
  {
    "reference": "MSDGIRIGVVGATGQVGVAMRQILAARRFPVDEIRFFSSPKSAGTVLSYEGRDVVVEDASTADPSGLDIALFSAGATASRALVPVFVDAGVIVVDNSSAFRKDPAIPLVVSEVNPEAAAAVIEAGRGIIANPNCTTMAAMPVLKPLHDEAGLQRLVVSTYQAVSGSGIAGVAELADGIAFAGDKAGELAYDGEAVAFPEPGVYQRTIAYNVLPFAGNLVDDGLNETDEEQKLRNESRKILGIPELPVSATNVRVPVFTGHSLSINAEFARPLSPERATELLAAAPGVELSDIPNPLQAAGKDPSYVGRIRRDEGVPDGRGLALFVSGDNLRKGAALNTIQIAELVAASR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The substrate binding site should be located in a region of the protein that is accessible to the ligand.2. The designed protein must be optimized for protein dimerization activity.3. The presence of Semialdehyde dehydrogenase NAD-binding domains is critical for the interaction of the designed protein with its intended targets.4. A protein should be able to manipulate the activity of enzymes in the L-threonine from L-aspartate: step 2/5 pathway, either by activating or inhibiting their function.5. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "LQPLHEKFGLKKVIVSTYQAVSGAGAKAVEELKEQSRAILNDEPFEASIMPCASDKKHYQIAFNAVPQIDKFEENGYTFEELKMMNETKKIMHLENLEIAATCVRLPVETGHSESLYAEFENGNPSVQEIRELLKTAPGITLQDQPEEQIYPMPAIAAGLPDVFVGRIRKDLDRDNAYHMWVVSDNLLD",
    "response#2": "LQPLHEKFGLKKVIVSTYQAVSGAGAKAVEELKEQSRAILNDEPFEASIMPCASDKKHYQIAFNAVPQIDKFEENGYTFEELKMMNETKKIMHLENLEIAATCVRLPVETGHSESLYAEFENGNPSVQEIRELLKTAPGITLQDQPEEQIYPMPAIAAGLPDVFVGRIRKDLDRDNAYHMWVVSDNLLA",
    "response#3": "LQPLHEKFGLKKVIVSTYQAVSGAGAKAVEELKEQSRAILNDEPFEASIMPCASDKKHYQIAFNAVPQIDKFEENGYTFEELKMMNETKKIMHLENLEIAATCVRLPVETGHSESLYAEFENGNPSVQEIRELLKTAPGITLQDQPEEQIYPMPAIAAGLPDVFVGRIRKDLDRDNAYHMWVVSDNLLE"
  },
  {
    "reference": "MVELGFPAYGPAIEKRVRPLYKALCDWRAAVTLAARRRFQQLRCNANMDDDGQPMFPPLPVPDWNNPSTDWRPSPPRSGPKKDFCGDLPAPLTSGPRLTTPSSGRMSELPHTTSSPRSSPRPRGPETSPSNEHIIISPPRNPPSNTTHRNVGHVSRSPSSSSSSSSSSSPSSSSLIVLSSPSSSRSPSPSPPRPRADSSSRPRRGRGSNRGGRSGPQSKGRKASPRTRKLEDEDYLPQETANRRGGGRPRGRPPKSGRAVQRNDIQVTSSSGLADTSPYDLCGSVWWEVPLPPPGRCWFGGLGGHRQALTDSPEIVEAIHRFNTSHGPVPVYVEEMKDYAKQYDALVNSLFHKSMKVNPLNWMHHGKLSPADAALNHIYVQKFQSSYDSPGAAVTGTVNRCIPHIAGAMKERKLLWAFPHIAASIAMTRRYCKDQKTFLFRSLKKAYASMAFPDNSSETEKGISSKPSTSPSVLITTSTQTTASPHAPKIDVTAIHGRVYQSVVDLSRCLADGSLDDPEFSFAGCTRPDCCVEEFDAARPLEELADACVLACDSVVAALLCGPDGPHRVAKMLSFYDSRITPHVPDSQQWEKCRILLVSWYNELSDLRAAVYGAYGNTPTSQHLDERALAARVVSLIAETIGPLVRQDPDRAWVRMGSRDITSLLLRDWRGTNDGDPGLVKAKHLRRTAELLNDGRSSKGTYGVFAPPRRPDQLFRGPGRPRRSTSSSQSASDKSPIKSTHRHTSDPIPISTPRPERDPAGTPHENTM",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Prioritize Basic and acidic residues, Polar residues compositional bias within the protein sequence to achieve desired characteristics.2. The protein must be able to DNA binding.3. The protein must exhibit the following characteristics: This IE protein is a multifunctional protein capable of migrating to the nucleus, binding to DNA, trans-activating other viral genes, and autoregulating its own synthesis. The designed protein sequence is ",
    "response#1": "TPVSTAMTTAKWLRNIISPLPSRPSTELLCFFSSCDRDITTDVTRRASIILGAIFPTTSFGDCCISGNLQSVNQMDSIWAEQRKVEAMKLYYRVLETMCRAESHILNGNNLTSLLSNDRFHRCMLACSAELVLATHKTVTMMFPAVLEKTGITAFDLSKVIESFVRHEETLPRELKRHLNSLEERLLESMAWERGSSMYNSC",
    "response#2": "TPVSTAMTTAKWLRNIISPLPSRPSTELLCFFSSCDRDITTDVTRRASIILGAIFPTTSFGDCCISGNLQSVNQMDSIWAEQRKVEAMKLYYRVLETMCRAESHILNGNNLTSLLSNDRFHRCMLACSAELVLATHKTVTMMFPAVLEKTGITAFDLSKVIESFVRHEETLPRELKRHLNSLEERLLESMAWERGSSMYNSC",
    "response#3": "TPVSTAMTTAKWLRNIISPLPSRPSTELLCFFSSCDRDITTDVTRRASIILGAIFPTTSFGDCCISGNLQSVNQMDSIWAEQRKVEAMKLYYRVLETMCRAESHILNGNNLTSLLSNDRFHRCMLACSAELVLATHKTVTMMFPAVLEKTGITAFDLSKVIESFVRHEETLPRELKRHLNSLEERLLESMAWERGSSMYNSC"
  },
  {
    "reference": "MMNILLPLLGGFIITAAFMPALIRYFRARHEGQMIREEGPTWHEKKSGTPTMGGLLFIVAIAVMTLLTSWVLAPHHMLPTTWILIFILVLYGALGMWDDSIKLFHRQNEGLKAWQKMLGQIVGALILFWVYTHEQLPMALHVPGIGNWHMSGWYAVFVILWLVGFSNAVNLSDGLDGLVSGLASIAFAAYGIVAWQQAQINIAIFCFAVVGSLLGFLIFNHKPAKIFMGDTGSLALGGALAAVSILLHHELSLLWIGLIFVIETASVILQVASFKLTGKRIFLMSPIHHHFEMKGWSEWRIDLTFWGIGLVTALSGVWVILAK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. A protein that can perform metal ion binding, phospho-N-acetylmuramoyl-pentapeptide-transferase activity in plasma membrane to support peptidoglycan biosynthetic process, cell cycle, regulation of cell shape, cell wall organization.3. A protein that is capable of catalyzing reactions within the Cell wall biogenesis, peptidoglycan biosynthesis pathway with high efficiency and specificity.4. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "YVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKALAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTVYLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRSRQAWILRQLG",
    "response#2": "LQRDWRSWEQLPDSLKVAVIAGEDQHFAEHHGFDLPAIQQALAHNERGGSIRGASTLSQQVAKNVFLWSGRSWLRKGLEAWFTLLIETLWTKQRILEVYLNSAEWGPGVFGAQAAARYHFGLDAERLSRTQASQLAAVLPSPLKWSAARPGPYVRQRAAWIRQQL",
    "response#3": "AAIAVDRHSGEIRAMVGGKRTGYDGFNRVLNASRQIGSLVKPAVYLTALAHPDQYNLATTLEDKPLTLKGSEGSAWTPRNYDRQYRGEVPLYLALAQSLNVPTVALGMKLGIDQVSATLGKLGVNRDEIRPVPSMLLGSFSLTPYQVAQMYQTLTNSGKKAPLSALRSVLDLEGNVLYESLPRVSQAVDQQAAWLTTYAMKQGVQEGTGRYLNAQFSSAALAGKTGTTNDNRDSWFVGVL"
  },
  {
    "reference": "MTRFVFITGGVVSSLGKGIAAASLGALLQARGYKVRLRKLDPYLNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTGVHATRADNWTTGRIYSDVIAAERRGHYLGGTVQVIPHITDKIKDVIQAETEGYDFVLVEVGGTVGDIESLPFLEAIRQLANELGPTRSLFVHLTLVPYIPSAGELKTKPTQHSVKELLGVGIQPQILLCRCDRQIPENERRKIALFCNVRPESVIPAYDADSIYAVPISYHEEGLDREVLRHFGLSIYGEPDMSRWQRIVDRIRAPEGEVTIAVVGKYTNLLDAYKSLAEALTHGGIAHNVKVRLDWVDSQIFEDDEAAVARLEKVNGILVPGGFGERGTEGKIEAVRFARERRVPFFGICFGMQMAVIEAARNQAGLKGASSTEFGPCTEPVVGLLTEWTRGNEVERRAAGGDLGGTMRLGAYPARLAEESLVREVYGGAAEIQERHRHRYEVNVNYKDRLEEVGLRFSGLSPDGSLPEIVERPDHPWFIGVQFHPELKSKPFDPHPLFRGFIGAAVRQARLV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should interact with enzymes and substrates within the CTP biosynthesis via de novo pathway, Pyrimidine metabolism, CTP from UDP: step 2/2 pathway to enhance or regulate its activity.2. The designed protein must possess glutaminase activity, CTP synthase activity, metal ion binding, ATP binding to support 'de novo' CTP biosynthetic process, glutamine metabolic process under certain conditions.3. The designed protein should contain one or more Glutamine amidotransferase domains that are essential for its function.4. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile; for glutamine hydrolysis, active site, Nucleophile to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).5. The L-glutamine, UTP, CTP, ATP binding site should be stable and able to withstand changes in environmental conditions.6. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen The designed protein sequence is ",
    "response#1": "MTRYIFVTGGVISGLGKGIVTSSIGKMLQFRGFKVTACKIDPYINYDAGTLRPTEHGEVWVTEDGGEIDQDLGHYERFLDINIPKSHNITTGQVFYEVIRKEREGKYLGQTVQPIPHVTDEIKRRIRKIAKESKADFVLVEIGGTVGDYENVLFLEAARQMRLEKEEVLYVHVAYLPIPKSLGEMKTKAVQHSVRELGRFGIQPDFIIGRANTPLDDVRKKKIALFCNVKEEDVISDPDIENIYKLPLIFEEEKFGDKILSKFGLKSKKTNLDAWKNFVNKIKNPKKTVRIAVVGKYTSLLDAYKSLNEALDHGGIANNVKVSVEWLDSQIFEREDTLQQLEGIHGILVPGGFGERGAEGKIKAAQFAREHKIPYFGICFGMQMAIIEAARNLAGLEDASSSEFGPMENPVVGLMTEWERAGTVERRNSASPMGGTMRLGAFPCDLLPGSKVHEIYKKEQIQERHRHRYEVNIHFKEQLEEVGVRFCGLSPDGQLPEMIELPDHPWFIGVQFHPELKSRPFEPHPLFTSFIKAALNS",
    "response#2": "MTRYIFVTGGVISGLGKGIVTSSIGKMLQFRGFKVTACKIDPYINYDAGTLRPTEHGEVWVTEDGGEIDQDLGHYERFLDINIPKSHNITTGQVFYEVIRKEREGKYLGQTVQPIPHVTDEIKRRIRKIAKESKADFVLVEIGGTVGDYENVLFLEAARQMRLEKEEVLYVHVAYLPIPKSLGEMKTKAVQHSVRELGRFGIQPDFIIGRANTPLDDVRKKKIALFCNVKEEDVISDPDIENIYKLPLIFEEEKFGDKILSKFGLSKKKANMAEWKDFLVKLKNPKKTVKIAVVGKYVDLKDAYLSLSEALCHGGIAHNTAVEIVWVDAEQVEQGDLGEIFSGVSGILVPGGFGGRGVAGKIRAINYARTRGVPFLGICMGMQCAVIEFARNVCGLKAANSTEFDPETPYPVIDLLPDQRNIEDLGGTMRLGLYPCRLLPGSRAAQAYGVEEVKERHRHRYEFNNRFRDLLEEKGLKVTGTSPDGVLVEVVEIPDHPWFLACQFHPEFKSRPTRPHPLFRAFVSAATLR",
    "response#3": "KFIFVTGGVVSGLGKGITASSLGALLKASGLKVFMQKFDPYLNVDPGTMSPYQHGEVFVTSDGGETDLDLGHYERFTDEELSKISSTSAGKIYLEVIEGERRGDSQGKTIQVVPHITDAIKHKVYLAAQRSKADVIISEIGGTVGDIESQPFIEAIRQIRMEQGKENVMFIHVVLLLWLAASKEYKTKPIQHSVRELLSLGIQPDVIVSRSDKSSPQEIKEKVSLFCNVPTENIIDAVDQDSIYRVPLAMAEQNLHKLVIDQLKVNIAVVGKYVKLRDADKSLCEALTHGGIANDTRVNIEWIEAESFEKEPHTVAERLKDKKAILVPGGYGERGTAGMISAIQYAREHQIPYMGICFGMQLAVIEVARHLAGIEKANTTEFGVTAEPVVGLLTEWLNGDVKERRSAEDDLGGTMRLGLYPAILAPGSLIQKIYGTSSIQERHRHRYELNIEYREKFEKAGLKFSGLSPDGLLPETVELEGHPWFVGVQFHPELRSRPFDPHPLFTSFIRALL"
  },
  {
    "reference": "MREAHTVEQVRAAEAARMADLPDGALMQQAAAGLAHAVVDLMGGAYGRRVLLLVGPGNNGGDALYAGARLARRGCSVEAWLLAGQVHEAGLEALRAAGGRVAEPRAGTTPDVVVDGIVGIGGRPGLRPAAVAALDHVRGVPVVAVDVPSGIDVDTGELAGPHVEATLTVTFGTLKVGHLVDPAATACGAVHPVDLGLDLPPAPVSALQPTDVRRLLPRPEPGGHKYTRGVVGVRAGSTTYPGAALLCVAGADCGLAGMVRHVGEVSDLVRREHPAVVGDGRVQAWVVGSGGGAAAGDELAAARSDGVPLVVDADALAHVGGPLGVPTVLTPHAGELAAMTGAERVDVEARMLHHARAAAATYDAVVLLKGRHTLVAHPDGRVRVTTTGPPWLATAGAGDVLAGLVGALLACGLDTYDAASVGSWLHGAAATLASRGGPLRAGDVAEAIPGTVRRLLDPGMEQSAP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein have enhanced (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.2. The designed protein must be optimized for ATP binding, NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity.3. The designed protein must have at least one YjeF N-terminal domain.4. The designed protein should be able to compete with endogenous proteins or inhibitors for AMP, K(+), (6S)-NADPHX binding.5. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.6. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. The designed protein sequence is ",
    "response#1": "MRLLDHAAIQGIGLPAAVLMENAGRAIAEEVLHLCRLCRFDKAEGHPGGRIIQRPPLAGLDLSASSSFPAELSRKGECEAVNVWKTEHWLILVGKGNNGGDGIVCGRHLAAAGIRVTLLYADPPAQFTGEAGLQRTIAEQLAIPHKVYERGTLRFTEYTGVVDALLGTGAKGAPRGMYAALIREVNDSGLPVVSADIPSGLNADTGEMHDPCIQAHRTVCLAFLKTGLTQYPGAGAAGEIVVRDIGIPEHLARHAHKYSVGLALVLGGSPGLTGAPVMASLAAARIGAGFVTCACASSIQPVLALKMTAITTLALPETADGGLDPDAALAALAPRLEKARALLVGPGLGRHPATQTFIRRLLTRTDLPTVIDADGLNALAGHLDDLAEQAAGRWILTPHAGEFRRLAGTDADLTDRIRTVQTCARRWNSVLLLKGLPGLVGTPDGRVYLNPTGNPAVATAGTGDVLAGLCVGLLAQGLPPGLCQWLVLGGSPGLTGAPVMASLAAARIGAGFVTCACASSIQPVLALKMTAITTLALPETADGGLDPDAALAALAPRLEKARALLVGPGLGRHPATQTFIRRLLTRTDLPTVIDADGLNALAGHLDDLAEQAAGRWILTPHAGEFRRLAGTDADLTDRIRTVQTCARRWNSVLLLKGLPGLVGTPDGRVYLNPTGNPAVATAGTGDVLAGLCVGLLAQGLPPARAAVAALYAGGAAADAYTTHRHPHTMTPLDLLEELPR",
    "response#2": "MRTAYRVQDVRAAEAALMARLPEGALMRRAAAGLAGACAGLLRRVYGARVVLLVGSGDNGGDALYAGALLARRGAGVSAVRLGRRVHEAGLDALVRAGGRVADDPYGVLARADLVVDGITGIGGRGGLRPEAVPVVRAARESGAVVVAVDLPSGVDADTGAVEGEAVRADATVTFGAYKPGLLVDPAREYAGALRLVDIGIAESDKYRRGVVGVVAGSARYPGAAVLAVAGALRGGAGAVRYVGPAGDAVLARYPETLVHPGPPGRAGRVQAWVIGPGLADTPGVADVLASDVPVLVDADGLRLLDPAAVRARPAPTLLTPHAGEAAALLGTVREDVEARRLDAVRELAGRYGATVLLKGSTTLVADPSPGVPVRVNPTGTPWLATAGSGDVLSGLTGSLLAAGLTARDAGSVGAYLHGQHADVARLLPVPAAESDKYRRGVVGVVAGSARYPGAAVLAVAGALRGGAGAVRYVGPAGDAVLARYPETLVHPGPPGRAGRVQAWVIGPGLADTPGVADVLASDVPVLVDADGLRLLDPAAVRARPAPTLLTPHAGEAAALLGTVREDVEARRLDAVRELAGRYGATVLLKGSTTLVADPSPGVPVRVNPTGTPWLATAGSGDVLSGLTGSLLAAGLTARDAGSVGAYLHGLAARRAATGGAGVSPTGAGAYPYDGGRAAPGAAGGAPLTAHEVARALPGAWRDVGVVAGSARYPGAAVLAVAGALRGGAGAVRYVGPAGDAVLARYPETLVHPGPPGRAGRVQAWVIGPGLADTPGVADVLASDVPVLVDADGLRLLDPAAVRARPAPTLLTPHAGEAAALLGTVREDVEARRLDAVRELAGRYGATVLLKGSTTLVADPSPGVPVRVNPTGTPWLATAGSGDVLSGLTGSLLAAGLTARDAGSVGAYLHGLAARRAA",
    "response#3": "MRLLDHAAIQGIGLPAAVLMENAGRAIAEEVLHLCRLCRFDKAEGHPGGRIIQRPPLAGLDLSASSSFPAELSRKGECEAVNVWKTEHWLILVGKGNNGGDGIVCGRHLAAAGIRVTLLYADPPAQFTGEAGLQRTIAEQLAIPHKVYERGTLRFTEYTGVVDALLGTGAKGAPRGMYAALIREVNDSGLPVVSADIPSGLNADTGEMHDPCIQAHRTVCLAFLKTGLTQYPGAGAAGEIVVRDIGIPEHLADDATITGWMPQRDRHAHKYSVGLALVLGGSPGLTGAPVMASLAAARIGAGFVTCACASSIQPVLALKMTAITTLALPETADGGLDPDAALAALAPRLEKARALLVGPGLGRHPATQTFIRRLLTRTDLPTVIDADGLNALAGHLDDLAEQAAGRWILTPHAGEFRRLAGTDADLTDRIRTVQTCARRWNSVLLLKGLPGLVGTPDGRVYLNPTGNPAVATAGTGDVLAGLCVGLLAQGLPPARAAVAALYAGGAAADAYTTHRHPHTMTPLDLLEELPRVLHTDDATITGWMPQRDRHAHKYSVGLALVLGGSPGLTGAPVMASLAAARIGAGFVTCACASSIQPVLALKMTAITTLALPETADGGLDPDAALAALAPRLEKARALLVGPGLGRHPATQTFIRRLLTRTDLPTVIDADGLNALAGHLDDLAEQAAGRWILTPHAGEFRRLAGTDADLTDRIRTVQTCARRWNSVLLLKGLPGLVGTPDGRVYLNPTGNPAVATAGTGDVLAGLCVGLLAQGLPPARAAVAALYAGGAAADAYTTHRHPHTMTPLDLLEELPRVLHTRF"
  },
  {
    "reference": "MGSTGEPDRKRRHFSSLSPTAATAKKMPFLPVSEDKKLDTAVLQYQNQKLKQKLEAQKVECSALENKFSQLKETQQSYNTTLTLVNKTWRELVDNLETCSVHLKDSASAGRHVKLPSTTEDGNSCLQDAFLSRLIETGATESCSANDFSDRMEEDRPTSCGKTKNSLSNIVSTINDLWCLKDGLYAAVLEALPEDGLCNKKISSDLHAEVNNMRLAFGDLHLKHKSVTRDMQSHRDIDAKNKAELKRLRGELESTVAELEESNCKLVTLKAERDAAKGAFFPILSLGSKNVAGDKARDKQKDLHDMEATLKELLDQSSSRLLELKALYEERIGILKQLSNLQNTLKNVKCISSSSAYVLVTDQLEKSKAEVVHYQALFEKLQVEKDNLVWREKEVNMKNDFVDVFRRSSVVTDSRLSELRIEIQNQINERNLIEIKLEEASREPGRKEIIAEFKALLSSFPDNMGTMQNQLRKYKEAASDVHSLRADVQSLSSVLERKEKELETLSTRSADQVADIRKLQALIQDLEESDIQLKLILEMYRCESVDSRDVLEARDKEYKAWAHVQSLKSSLNEHSLELRVKTAIEAEALSQQRLAAAEAVIVDLRQKLEASKRDMFRLSDVLKSKHEENEAYLSEIETIGQAYDDMQTQNQHLLQQITERDDYNIKLVLEGVRSRQLQDSLLMEKQTMERGFQRATTSLGFFDMKAGRIEDQLKMCSDQVQKLAEDRLQSLGTLANFQKRLLDVTRLSQQARESLEESQSKVDKSRVS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should emphasize Basic and acidic residues compositional bias.2. The protein contains novel RING-type domains that confer a unique function or activity.3. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "QLTDLQEAHFVVFESEENSESVMDGFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIPDKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKD",
    "response#2": "QLTDLQEAHFVVFESEENSESVMDGFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIPDKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDC",
    "response#3": "QLTDLQEAHFVVFESEENSESVMDGFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIPDKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDD"
  },
  {
    "reference": "MIAITLPDGSRREFPGPVTVAEVAQSIGAGLAKAALAGRIDGQLVDTSFRIEQNVDLAIVTDKDTDGLDVIRHSTAHLLAYAVKELYPEAQVTIGPVIENGFYYDFAYKRPFTPEDLVAIEKRMAELAKKDEKVTREVWSRDDAVKLFEGMGEKYKAEIIASIPEDQEIGLYREGNFVDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDSNNEMLQRIYGTAWAKKEDQEAYLHMLEEAEKRDHRKLGKLLDLFHLQEEAPGMVFWHPKGWQIWQAVEQYMRGRLAGAGYSEVRTPQVMDRGLWERSGHWENYKENMFVTESEKREYAIKPMNCPGHVQIFNHGLRSYRDLPLRLAEFGACHRNEPSGALHGLMRVRGFVQDDAHIFCTEGQIMAEAKDFNDLAFSIYEDFGFENVAVKLALRPDKRAGTDEIWDHAEEGLRTALRACGVEWEELPGEGAFYGPKVEYHIKDAIGRSWQCGTLQLDLVLPERLGAEYVAEDNSRKRPVMLHRAILGSFERFLGILLENHAGALPLWLAPEQVVIMSIADAHAEYAESVAQSLQKQGFRASADLRNEKITYKIREHSLQKVPYLVVVGDKERDGNQVAVRARGNVDLGSMPVSTFVERLQNDFANKS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Also edits incorrectly charged L-seryl-tRNA(Thr)..2. The designed protein must have at least one TGS domain.3. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.4. The designed protein should be involved in threonyl-tRNA aminoacylation by localizing to cytoplasm and exhibiting tRNA binding, threonine-tRNA ligase activity. The designed protein sequence is ",
    "response#1": "IDDNQYAIKPMNCPGGMLLYKEKRYSYRDLPLRVAEIGLVHRHELSGVLSGLFRVRAFHQDDAHIFMTPDQIEQEIFGVLKLVETIYSTFGLGFHLELSTRPAKSIGTDEQWVKATAGLRAALDAYGKGYAVNEGDGAFYGPKIDVHIKDALGRTWQCGTIQLDMSLPERFDLSYIDAGSEKQRPVMIHRVIYGSIERFLGILIEHR",
    "response#2": "DHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLERYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNCPHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQIQEEFQRALQLIIDVYADFNLTDYRFRLSYRDPNDTHKYYDNDEMWENAQSMLKAALDEMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEEHRPVMIHRGVISTMERFTAILIETYKGAFD",
    "response#3": "HPNGMVVLNELEKFLKEQLLERGYGQIKTPLILNEHLWHQSGHWDHYKENMYFTKIDGEDYAIKPMNCPGSILVYKDELHSYRELPIKVAELGQVHRHELSGALHGLFRVRTFVQDDAHVFCLPEQIEEEVSKTIDFCDYIYSKFGFKYEVELSTRPEDSMGSDEDWDLAISSLKNALEHKGLPYKINEGDGAFYGPKIDFHLEDAIGRTWQCGTIQLDFQMPERFDMTYIASDGSKKRPAMIHRAILGSEERFMGILIEHYAGKFK"
  },
  {
    "reference": "MASSLLTTPSQTLAPNPAAARARRSSPAAAQVSFSSPLLPGRRALRCARPVAIEKEVPEKEAPTTFLREDGSGAGSGSVRERFEGMIRRVQGEICAALEEADGSGKRFVEDVWSRPGGVCVHSRVLQDGNVFEKAGVNVSAVIGVCPRSAYRAAKGAAKNGAADGHKAGPVPFFSAGISSVLHPKNPFAPTLHFNYRYFETDAPKDVPGAPRSWWFGGGTDLTPSYLIEEDVKHFHSVQKQTCDKFDPSFYPRFKKWCDDYFYIKHRNERRGLGGIFFDDLNDYDQDMLLNFATECAGSVIPAYIPIIERRKDTPFNEEQKAWQQVRRGRYVEFNLVYDRGTTFGLKTGGRIESILVSLPLTARWEYDHKPEEGSEEWKLLDACINPKEWL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. We are looking for a protein with broad substrate specificity for coproporphyrinogen III + 2 H(+) + O2 = 2 CO2 + 2 H2O +   protoporphyrinogen IX.2. The protein design should consider the endogenous proteins and enzymes present in the Porphyrin-containing compound metabolism pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "DSAFLKAARGEPVPHTPVWYMRQAGRSLPEYLAVREGIGMLESCMDPDLVVEITLQPVRRYGVDAAIFFSDIVLPLKAVGVDLDIKPGVGPVVAHPVRTLADVEAIPDLTPEHVPFITEAVRGLVGELGGTPLIGFAGAPFTVASYLVEGGPSKEHARTKAMMFGAPDVWDALLRKIAGISASYLEVQVAAGASAVQLFDSWAGALTPADYRAFVMPHSARVLERAGALGVPRIHFGVGTANLLGLMGEAGADVVGVDWRTPLAHAIALVGDRGVQGNLDPTLVFAPTEVMTARAAEIVEAGRAARGHIFNLGHGVIPSTDPDQLKRLTEFVQTR",
    "response#2": "ISGLAVAHHLRSRGTDAVLLESSARLGGAVGTHALAGYLVEQGPNSFLDREPATRALAAALNLEGRIRAADPAAKRRYVYTRGRLRSVPASPPAFLASDILPLGARLRVAGELFSRRAPEGVDESLAAFGRRHLGHRATQVLLDAVQTGIYAGDVEQLSVAATFPMLVKMEREHRSLILGAIRAQKAQRQAALPAGTAPKLSGALSTFDGGLQVLIDALAASLGDAAHVGARVEGLAREDGGWRLIIEEHGRRAELSVAQVVLAAPAHATAKLLRPLDDALAALVAGIAYAPIAVVHLGFDAGTLPAPDGFGFLVPAEEQRRMLGAIHASTTFPFRAEGGRVLYSCMVGGARQPGLVEQDEDALAALAREELKALAGVTARPSFTRVFRWPLGIPQYNLGHLERVAAIDAALQRLPGLHLIGNAYKGVGLNDCIRNAAQLADAVS",
    "response#3": "SEKTILRALKGERLPVPPVWMMRQAGRYLPEYRATRAQAGDFLSLCYTPDLAAEVTLQPIRRYGFDAAILFADILLLPQALGLDLWFVTGEGPRLSTVTSAAGVAALKPAAAIHDTLSPVYETVRILARELPRETTLIGFAGAPWTVATYMVAGRGTPDQAPAHAFKAEDRAAFAALIDRITEATIDYLSAQIEAGAEVVKLFDSWAGSLKGRDFDDFAVEPARRIIAALKARHPGIPVIAFPREAGPRYAGFARATGADCVALDNSVSADWAAAEVQKDGCVQGNLDPRLLVSGGEELVSETRRIVQAFSRGPHIFNLGHGITPEADPENVARMLEAIR"
  },
  {
    "reference": "MREKIVLAYSGGLDTSIIIPWLKENYSCDVIAMVGDVGQGDDIDAVVAKAHKTGASKVIVKDLREEFLTQYVYPAISTGAVYEHKYLLGTSLARPPIAKAQVEVALAEGATAVSHGCTGKGNDQVRFEHAFQALAPELKIIAPWREWTLKSREDCLDYAEAHGISVAQSREKIHSRDRNLLHVSHEGGELEDPNNAPLDTTWTWTKSPQEAPDRVEEVTIGFEGGVPVSINGMKLEPLALIELLNEIGARNAIGRIDLVENRFVGIKSRGCYETPGGSLLLAAHRELEALCLDRDTLHYKQEVALKWAELVYFGLWFTPLRESLDAFVASTQKNIAGAVKLALYKGNIAVAGRTSPKSLYRPDIASFTMGAGYDQKDAEGFIRILGLPARSRALIENAGKEKVSK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. We need a protein that can perform ATP + L-aspartate + L-citrulline = 2-(N(omega)-L-   arginino)succinate + AMP + diphosphate + H(+) with high efficiency.2. The designed protein should have a binding site that is specific to the target ATP ligand.3. The protein can be targeted to cytoplasm and perform ATP binding, argininosuccinate synthase activity.4. A protein that can modulate the overall flux of metabolites through the L-arginine biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "FSVDANLLHTSSEGKALENPADEAPDYVYQRTVSPEDAPDQPEYIEVSFERGDAVAINGEALSPATILTALNEYGRKHGIGRLDFVENRFVGMKSRGIYETPGGTILLEAHRGIEQITLDSGAGHLKDSIMPRYAELIYNGFWYSPEREMLQALIDKSQEHVTGTVRLKLYKGGVHTVGRWSEHSLYSEKHVTFEDDAGAYDQKDAAGFIRLNALRLKYSIDENLWGRSIEGDIISDPASEVPEDAFEWTAVRKQDKLKLSVEFEKGVPVRVNGEKLDPVKLISLLNEEVGSRGFGRVEHLENRVVGFKSREVYEAPAALALIAAHKDLEKTVLTPLELRFKRHLDSLWSDLVYQGLWYEPLRNTLELAGDEINKWVSGEVKLEVDLKSLRVVGRTSPYSPYSEKISSYNKGWYPSDEEARGFIEIWGMHN",
    "response#2": "FSVDANLLHTSSEGKALENPADEAPDYVYQRTVSPEDAPDQPEYIEVSFERGDAVAINGEALSPATILTALNEYGRKHGIGRLDFVENRFVGMKSRGIYETPGGTILLEAHRGIEQITLDSGAGHLKDSIMPRYAELIYNGFWYSPEREMLQALIDKSQEHVTGTVRLKLYKGGVHTVGRWSEHSLYSEKHVTFEDDAGAYDQKDAAGFIRLNALRLKYSIDENLWGRSIEGDIISDPASEVPEDAFEWTAVRKQDKLKLSVEFEKGVPVRVNGEKLDPVKLISLLNEEVGSRGFGRVEHLENRVVGFKSREVYEAPAALALIAAHKDLEKTVLTPLELRFKRHLDSLWSDLVYQGLWYEPLRNTLELAGDEINKWVSGEVKLEVDLKSLRVVGRTSPYSPYSEKISSYNKGWYPSDEEARGFIEIWGMHA",
    "response#3": "FSVDANLLHTSSEGKALENPADEAPDYVYQRTVSPEDAPDQPEYIEVSFERGDAVAINGEALSPATILTALNEYGRKHGIGRLDFVENRFVGMKSRGIYETPGGTILLEAHRGIEQITLDSGAGHLKDSIMPRYAELIYNGFWYSPEREMLQALIDKSQEHVTGTVRLKLYKGGVHTVGRWSEHSLYSEKHVTFEDDAGAYDQKDAAGFIRLNALRLKYSIDENLWGRSIEGDIISDPASEVPEDAFEWTAVRKQDKLKLSVEFEKGVPVRVNGEKLDPVKLISLLNEEVGSRGFGRVEHLENRVVGFKSREVYEAPAALALIAAHKDLEKTVLTPLELRFKRHLDSLWSDLVYQGLWYEPLRNTLELAGDEINKWVSGEVKLEVDLKSLRVVGRTSPYSPYSEKISSYNKGWYPSDEEARGFIEIWGMHL"
  },
  {
    "reference": "MSSLKRIVWFPILAIAIQILSIHTVLSQSQNNAFLFHKCSDIEGSFTSKSLYESNLNNLFSQLSYKVPSTGFAASSTGNTPNNVNGLALCRGDASSSDCRSCLETAIPELRQRCPNNKAGIVWYDNCLVKYSSTNFFGKIDFENRFYLYNVKNVSDPSTFNSQTKALLTELTKKATTRDNQKLFATGEKNIGKNKLYGLVQCTRDLKSITCKACLNGIIGELPNCCDGKEGGRVVGGSCNFRYEIYPFVKTA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein localizes to the cytosol, plant-type vacuole. The designed protein sequence is ",
    "response#1": "WGLARISHKSVKYDDIGKYVYDSSAGDNITAYVVDTGVSIHHVEFEGRASWGATIPSGDVDEDNNGHGTHVAGTIASRAYGVAKKAEIVAVKVLRSSGSGTMADVIAGVEWTVRHHKSSGKKTSVGNMSLGGGNSFVLDMAVDSAVTNGVIYAVAAGNEYDDACYSSPAASKKAITVGASTINDQMAYFSNYGSCVDIFAPGLNILSTWIGSNTSTNTISGTSMATPHVAGLSAYYLGLHPAASASEVKDAIIKMGE",
    "response#2": "WGLARISHKSVKYDDIGKYVYDSSAGDNITAYVVDTGVSIHHVEFEGRASWGATIPSGDVDEDNNGHGTHVAGTIASRAYGVAKKAEIVAVKVLRSSGSGTMADVIAGVEWTVRHHKSSGKKTSVGNMSLGGGNSFVLDMAVDSAVTNGVIYAVAAGNEYDDACYSSPAASKKAITVGASTINDQMAYFSNYGSCVDIFAPGLNILSTWIGSNTSTNTISGTSMATPHVAGLSAYYLGLHPAASASEVKDAIIKMGW",
    "response#3": "VAFGKKLKERSIQEWQGYYINYKLMKKKVKQYSRQLEGGNLERRHVLKDFSRMLDNQIEKIALFMLEQQGLLASRLQTLRGSHDALQEQPDISHMSYLKEEYRAVGQDLLKLLFFVEMNAIGIRKILKKFDKRFGYRFTNYYVC"
  },
  {
    "reference": "MSFFERLSQRVRQVDSLLCVGLDPHPDDLPTPTAESSREFCLRLIQATSDIAAAYKPNIAFFEAFGSAGWSALQQVIQSIPENIPVIVDAKRGDIASTAEAYALSIFTLLGAQAVTINPYLGHDSIEPFLADPERGVFLLCKTSNPGAGDLQDLLLEETLPSSKGEVAYHLYEKVAQLAQEWNTRDNLGLVVGATDTQALHRVRLLAPELWFLAPGVGAQGGNLETALQAGLRSDGMGLLVPVSRGISRAADARVAADEIRQKINDQRKNIQKLTQASPQLMGRPSASTEIAQLADGLLEAGCIKFGKYTLKSGLLSPIYVDLRQLVSFPKFLMQVARAYLPLLRNISFDRIAGLPFAALPIATAISIQGGWPLIYPRKEAKTYGTKAEIEGLYREGERVVVIDDLTTTGGSKLEAIDKLTSAGLKVEDVVVLIDRQSGAVEALAEAGFKLHSVVTLTGLLDHYEKSGKVPLSQIEAARAFISKSL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be able to modulate 'de novo' UMP biosynthetic process in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "CVGLDTSLDYIPEEFKSKFSNESDMLFAFNKEIIDATLDVSACFKVQIAYYEALGLKGLEAYKNTLSYLREKNALIIADIKRGDIAATAKMYAKAHFEGDFESDFITLNPYMGMDSIDPYLPYIEKNEKGVFVLVRTSNKGAQDIEYLEAGDGKKVYDVVGEKLNTLGKNYLGKHGYSSIGGVVGCTHQEEAKEMRDKLDTMPFLIPGYGAQGGTAKDVASYLKNGNGGIVNSSRKILLAE",
    "response#2": "VKIAVVGKYIDLQDAYKSIYESLTHAAASQDCGIDLKLVDAEALRDGVDGHLKDVAGILIPGGFGERGVEGKIKAAKFARENKLPYLGLCLGMQVATIEFARNVCGIEQANSTEFDKDAPDPVISLLEEQRSVQNKGASMRLGTWPTKIEPGTLAQKVYGSDEVLERHRHRYEFNMDYRDRMREKGFEISGTSPDGSLAELVELRDHPYFLACQYHPEFQSRPNKPHPLFRGFVR",
    "response#3": "CVGLDTSLDYIPEEFKSKFSNESDMLFAFNKEIIDATLDVSACFKVQIAYYEALGLKGLEAYKNTLSYLREKNALIIADIKRGDIAATAKMYAKAHFEGDFESDFITLNPYMGMDSIDPYLPYIEKNEKGVFVLVRTSNKGAQDIEYLEAGDGKKVYDVVGEKLNTLGKNYLGKHGYSSIGGVVGCTHQEEAKEMRDKLDTMPFLIPGYGAQGGTAKDVASYLKNGNGGIVNSSRKILLAV"
  },
  {
    "reference": "MANTSSAKKATRKIARRAAINKNRRSRVRTYVRQVEEALASGDKAAAQAAFKAAEPELMRAATKGVIHKNTASRKVSRLAARLKVLSA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be involved in translation by localizing to ribonucleoprotein complex, ribosome and exhibiting rRNA binding, structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "IPPTTALVMKEAGVEKGSGTPNTQAVGNLPLEAVIRIAKMKMESMLSYDLKTAAKEVMGTCVSVGVTL",
    "response#2": "IPPTTALVMKEAGVEKGSGTPNTQAVGNLPLEAVIRIAKMKMESMLSYDLKTAAKEVMGTCVSVGVTL",
    "response#3": "GGDKVVVLAGKDKGKRGER"
  },
  {
    "reference": "MMVMLYNLFNQQNLAETKISYTEFLAMVEGERVSDVVIQGQELYVTDINRNRLKVYAPQDMDLIKILRQKKVPISAKPPAESPWYMSVLVSWFPMIVLIGVWIFFMRQMQSGGGKAMSFGKSRARLQSDQSKKVTFDDVAGIDEAKEELGEIVEFLKDPKKFTRLGGRIPKGVLLMGSPGTGKTLLARAIAGEADVPFFHISGSDFVEMFVGVGASRVRDLFVQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFESNEGVILISATNRPDVLDPALLRPGRFDRQVVVPLPDIVGREKIIRVHLKKTPVGPDVDPLLLAKGTPGFSGADLENLVNEAALLGAKRDKESIEMSDLEDAKDKVYMGLERKSKVIREEDRKTTAYHEGGHALVARFLPGTDTVNKITIIPRGRAMGITWFLPEESDFKYKDQLESELSVALGGRAAEEIVFNRISTGAANDIKQATDVAQKMVRSWGMSTELGLLSYAKGEEHIFLGREIAQHRDYSEETARKIDREISNLIKKAYKLAKSTLEQNVDILHALAERLLEKETVMGKELDELIRTMKPGFEFPSKSIEMEAKDKDTKDPGETSAEKKAEAETEAGTEPKVEAKAEAADE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The ATP binding site should be stable and able to withstand changes in environmental conditions.2. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins3. The designed protein must possess ATP hydrolysis activity, metalloendopeptidase activity to support protein catabolic process under certain conditions. The designed protein sequence is ",
    "response#1": "VLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFENAKKNAPSIIFIDEIDAVGRRRGAGMGGGHDEREQTLNQMLVEMDGFEGDEGVIVMAATNRSDVLDPALLRPGRFDRKILVHLG",
    "response#2": "IPRGVLLVGDPGCGKTLLAKAIAGEADVPFFSISGSDFVEMFVGVGASRVRDLFKQAKESAPCIIFLDEIDAVGRRRGGGFSGGGNDEREQTLNAILVEMDGFTSSDGVIVIAATNRGDVLDPALTRPGRFDRQITVPLPYVET",
    "response#3": "VLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFENAKKNAPSIIFIDEIDAVGRRRGAGMGGGHDEREQTLNQMLVEMDGFEGDEGVIVMAATNRSDVLDPALLRPGRFDRKILVA"
  },
  {
    "reference": "MDNKVDLKNFTLEELEVFLAGQGKEKYRARQIFKWLYQKDAATFEEMTNISKELRRELAESAFISSLAPETVEVSRDGTRKYLFRLADGNSIESVLIPEEDRSTLCISSQVGCAMACEFCLTGTFKFTRNLTTAEIVNQVCAVKREAEVRNVVFMGMGEPLANLENVIRALKIIISPDGMQFSSRRVTVSTAGLVPEMGLLGKEVAVNLAVSLNATTDELRDRLMPVNRRYPLAELLAACKRFPLPARRMITFEYVMINGVNDSIDDAKRLVRLISHIPAKVNLIPFNEHEGCSYRKPEQEAIDRVHKFLLDKHITVITRSSRGADISAACGQLKGKLEKICSNSE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.2. The [4Fe-4S] cluster, S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions.3. The designed protein should be involved in rRNA base methylation by localizing to cytoplasm and exhibiting rRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding, metal ion binding, tRNA binding.4. The protein contains novel Radical SAM core domains that confer a unique function or activity.5. The design of the protein must focus on the precise arrangement of amino acids in the S-methylcysteine intermediate, Proton acceptor to enable effective catalysis of adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "ETDRGTLCVSSQAGCAVGCRFCSTGHQGFSRNLSTAEIIAQLWFAEHFLRKKLGTSERVISNVVMMGMGEPLQNYAALIPALRTMLDDHGYGLSRRRVTVSTSGVVPMMERLGVDCPVALAVSLHAPNDALRDHLVPLNKKYPLSELMHACHQYLTHAPRDFVTFEYCMLDGVNDTQEHAQQLIHLVQQDGRGTSWCKFNLIPFNPFPASGLRRSPHAAVQAFAARLNAASIVTTVRKTRGDDIDAACGQLAGDVKGRMVREARLESQAATSGP",
    "response#2": "EEGRGTLCISSQVGCTLTCSFCHTGTQKLVRNLTSEEILAQLLVARDRLGDYPEIKGTDTPRKPTEAMVPSEGRLVSNIVMMGMGEPLYNFDNVKDALLIAADGDGLSLSKRRITLSTSGVVPMIPKAGDEIGVMLAISLHAVRDDLRDELVPLNKKFPIKDLLDACRAYPGLSNAKRITFEYVMLKGVNDNPADARELVRLLRGIPAKINLIPFNPWPGSPYECSEWDAIERFADIVNHAGYASPILSPYKGDVGASIEIEAAEERLLAFGVKNYHAKSFTHFVYQLGVLEPSKMLSLSRDFRKRLQDCVDTRLLPIKKRLVSKDGTVKFLFEVRGPKSLEEIEAVYIPEKSRVTLCVSSQVGCKMGCRFCLTAQLGFRANLSAGDIVRQVMTVNADPELRPVTNIVFMGMGEPFDNLDEVRKATKILTHERGLKLSARKICVSTVGLADRIDSLTKEEPFRLAVSLNSSDDVSRNEVMPINRRWNLERLLESCLRYSQRTNKKVTFEYILMKGVTDRDEDLRNLVRLLSPIPCKLNLIPYNESPFTPFKRPTEERILEFHAGLLKAQFPVFIRKNRGNDIFAACGMLKRSDTPTNLYDLTRPALGALLSDWGFRPHHRDVLWTGLYRQQVRALDEVEGLRSDLRDTLRERTRLSHLATHHEAFSSDGHTHKLLLRLEDGQTIETVLMRFKGRATVCISTQAGCAMGCVFCATGQMGLSRHLTPGEIVGQILHVQRILRETGESLRNVVLMGMGEPLHNYDNTLAAVDVLVDPLGLAMGPRFITLSTVGVVPGIRRLADEDRPVQLAVSLHGATDAERAALVPAGRRWPLAELMDACRYYIARRKRRIFFEWTLIAGRNDTSEHAHTLGALLGGMDAHVNVIPLNPTVGYDGDPSQPASVRAFQDVLMSHGVPSTVRQRRGIDIDAGCGQLKSAVERRSR",
    "response#3": "MSDTPTNLYDLTRPALGALLSDWGFRPHHRDVLWTGLYRQQVRALDEVEGLRSDLRDTLRERTRLSHLATHHEAFSSDGHTHKLLLRLEDGQTIETVLMRFKGRATVCISTQAGCAMGCVFCATGQMGLSRHLTPGEIVGQILHVQRILRETGESLRNVVLMGMGEPLHNYDNTLAAVDVLVDPLGLAMGPRFITLSTVGVVPGIRRLADEDRPVQLAVSLHGATDAERAALVPAGRRWPLAELMDACRYYIARRKRRIFFEWTLIAGRNDTSEHAHTLGALLGGMDAHVNVIPLNPTVGYDGDPSQPASVRAFQDVLMSHGVPSTVRQRRGIDIDAGCGQLKSAVERRSREEVARERALVSSQVGCAMGCVFCASGVAGLKRQMTDAEIVAQVLLGRRELRPDQRIRNVVFMGMGEPLHNYDAVARSLVLLTHPDGIDLSKRRVTVSTSGLVKQIDRLGKDFDGQVQLAVSLHAVRDEDRSKIMPVNRKHGISELTDALRRYPMSKRARITIEYTLIKDVNDGVESTHPLIPPSTQVGCAMGCAFCATGSMGLVRHLAAGEIAGQVEAVRRLLAAEGLERPVSNVVFMGMGEPLHNLEATLAAVEILLHPLGGGMSRRHLTVSTAGLVPAMKKFVERVPAKLAVSLNAADDEVRSRLMPINRRYPLERLLAACRELNLKHTDRITFEYVLLGGINDGDDDARP"
  },
  {
    "reference": "MDPVDPSLEPWNHPGSQPATPCTKCYCKKCCFHCPYCFLNKGLGISYGRKKRRQRRAASHGGKSDQDPIPKQPSSHNNRGDPTGPKE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to promote processive transcription elongation by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts; Tat can reactivate a latently infected cell by penetrating in it and transactivating its LTR promoter; The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive; This also contributes to the increase in proviral transcription rate, especially when the provirus integrates in transcriptionally silent region of the host genome; In the absence of Tat, the RNA Pol II generates short or non-processive transcripts that terminate at approximately 60 bp from the initiation site; Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication2. The protein design should include a Nuclear localization signal, RNA-binding (TAR), and protein transduction, Cell attachment site motif to ensure proper function.3. A protein that can perform metal ion binding, trans-activation response element binding, actinin binding, protein domain specific binding in host cell nucleolus, host cell cytoplasm to support suppression by virus of host type I interferon-mediated signaling pathway, negative regulation of peptidyl-threonine phosphorylation.4. The protein should have a high affinity for Zn(2+). The designed protein sequence is ",
    "response#1": "MEPVDPNLEPWNHPGSQPSTACNNCYCKRCSYHCQLCFLKKGLGISYGRKKRRQRRRAPQSSEDHQNLVSKQPLPRTQGDQTGSEESKKKVESKTEPDPFD",
    "response#2": "MEPVDPRLEPWKHPGSQPKTACTNCYCKRCCFHCQVCFITKGLGISYGRKKRRQRRRAPQDSKTHQASLPKQPASQPRGDPTGPKESKKKVERETETDPSH",
    "response#3": "MEPVDPKLEPWNHPGSQPKTACNNCYCKRCSYHCQVCFLKKGLGISYGRKKRRRRRSPPQSSEDHQNPVSKQPISQTQGDSTGPKESKKKVESKTEANPFD"
  },
  {
    "reference": "MSPWHERSNGEKLTPIIDFASVQRLKASMNPRRTMTVHRQVCIIGAGPCGLTTVKNLVQAGVTDIVCHEALPETGGIWAYSTDPDRPSVYESAHTISSKALSQFRDFPIPETYPDFPSHRQVLDYMRAYAEEFGLADHIRLNSRIESARRDDQGIWHIEGRGSDGPFHETADHLIVCSGHHREAELPELKGSFDGERIHSGRYRRTAPFAGKRVLVVGAGNSGCDIAAALSRVAEHVSLSVRSPQHIIPKLMFGRPVDMQYAKIQNFPGFLRRALVSLGLRLMIGPYRKYGLAEPDSSPLAMHPTLNSDILEQFRHGRVIARPGIKEAEGDIIRFVDGSEGRFDTIIYATGYKIDFPFLDPADCVWADEVDVPLYLKIMPEAVDNLYFVGLIQPIGCIWALADLQAGLVARKIAGTWRRPRDIDRRIAQEMRRDRQQFVRSRRHAVEVDFHDYRREIARATAEVG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have flavin adenine dinucleotide binding to facilitate lipid metabolic process. The designed protein sequence is ",
    "response#1": "FDLTIIGGGPAGLFAAFYAGMRKMKVKVIESMPQLGGQLSALYPDKYIYDVAGFPKVKAQDLVDQLTAQAQQFQPEIVLEEAVQTLEKQEDETFVLTTDANIHYTKAVLITAGAGAFAPRKLQVEKADFYEDKNIHYFIRDLSAFTGKRVVVIGGGDSAVDWALMLEPIAKEVTLVHRRDAFRAHEHSVDLLKKSSVRILTPYETAELHGDENGVTAVTFSEVKGDQTETIAVDDVIVNFGFVSTLGPIKEWGLEIRKNAIPVNTKMETNIPGVYAAGDVSTYDGKIKLIATGFDARWEVFVALRDGIEAERERLARTVVGPAPEVQRVLERLGSAPLKAGVTLEQLLRRPEVRYEHLVEMGLGRPDLDEAIWREVETQVKYAGYIAKEV",
    "response#2": "GFDLTIIGGGPAGLFAAFYAGMRKMKVKVIESMPQLGGQLSALYPDKYIYDVAGFPKVKAQDLVDQLTAQAQQFQPEIVLEEAVQTLEKQEDETFVLTTDANIHYTKAVLITAGAGAFAPRKLQVEKADFYEDKNIHYFIRDLSAFTGKRVVVIGGGDSAVDWALMLEPIAKEVTLVHRRDAFRAHEHSVDLLKKSSVRILTPYETAELHGDENGVTAVTFSEVKGDQTETIAVDDVIVNFGFVSTLGPIKEWGLEIRKNAIPVNTKMETNIPGVYAAGDVSTYDGKIKLIATGFG",
    "response#3": "FDLTIIGGGPAGLFAAFYAGMRKMKVKVIESMPQLGGQLSALYPDKYIYDVAGFPKVKAQDLVDQLTAQAQQFQPEIVLEEAVQTLEKQEDETFVLTTDANIHYTKAVLITAGAGAFAPRKLQVEKADFYEDKNIHYFIRDLSAFTGKRVVVIGGGDSAVDWALMLEPIAKEVTLVHRRDAFRAHEHSVDLLKKSSVRILTPYETAELHGDENGVTAVTFSEVKGDQTETIAVDDVIVNFGFVSTLGPIKEWGLEIRKNAIPVNTKMETNIPGVYAAGDVSTYDGKIKLIATGFEF"
  },
  {
    "reference": "MEMSGLGRKRVSKWDSKEDTHHHHSSVNANSASYYRDKESEPVRFNAESNGEARTRSRVSQNNDNSYFSEQDGTRQQFVRRSGSRSNSRSRSRSRSPVYRARRDAGSYDRHKTRTQVSPTPIREFNKRGSDHLFDQSRSDHYGWEDNIRKPRETKYHTDDFREEAMMEGARSSDYNNTDYPEDNSRIRRRRSEFTGEKETQRRDGGDGEGGFHRSSNIPCKFFAAGTGFCRNGKYCRFSHHVADRKQPQDNNNNFYRQDNNNHTSGHNKWNDVERLDNGRVGGIEVSRASKKGVSESKGNGSSSWIDDMEMSPDWNYGVQALKKPVKEDHSVGIIGQSSQSRVLKDDQRSSGMFSHGGRTMAEKPVAASHQSYSNSVNVAPVETFNQNHNALPYQSSLTAGGSQQVLAAAATNFSVGSNLSNLESGKVYQDNHHSTVEKPVLVQNTVSREQIDQITNISASLAQFLANGQPIPQLEQALQLPLHSESVQPNQATTQSNVVSSNPNQLWGLGMSTGAEGVPAVTASKISNVEEIQEVSLDPKENGDKKTDEASKEEEGKKTGEDTNDAENVVDEDEDGDDDGSDEENKKEKDPKGMRAFKFALVEVVKELLKPAWKEGKLNKDGYKNIVKKVAEKVTGTMQSGNVPQTQEKIDHYLSASKPKLTKLVQAYVGKIKKT",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "QKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEAX",
    "response#2": "DMKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEAL",
    "response#3": "DMKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEAN"
  },
  {
    "reference": "MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLYTSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFDKDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTNIKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQSFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLNLARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQLMTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKARTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. A protein that contributes to establishment of competence for transformation by functioning in plasma membrane with ATP binding. The designed protein sequence is ",
    "response#1": "NVYLLGFTSFLNDMSSEMIMPILPMLITSVGGGSLSIGLVGGLREFISNILMVLIGYCSDKVRKRKIFVVLGYLTSSMFKLLLGLSKSWLGAVIFSSLERMGKGIRTAPRDAIISESMPKTLGKGFGIQRAFDTAGAILGSTLSLLFILYLQYSFNQIILIAAVIGFLTLIPLYFVKEKPSPSNNKITFRVGIKNLPKELKLFILISAIFTLSNFSYMFYILRAQEFLMIVDEKMAIIIPIALYILYNIFYATFSIPFGILSDKIGRKSVLTIGYIVYGIVSLGFAYFISQKSLILLFALYGIAYALFAGNQKAYVSDLSSEDIRATALGLFYTVVGLTSLPASLIAGYLWKISPEMTFLYGSVLAIISGLLLLFTR",
    "response#2": "LELHGALFSWDPVGTSLETFISHLEVKKGMLVGIVGKVGCGKSSLLAAIAGELHRLRGHVAVRGLSKGFGLATQEPWIQFATIRDNILFGKTFDAQLYKEVLEACALNDDLSILPAGDQTEVGEKGVTLSGGQRARIALARAVYQEKELYLLDDPLAAVDADVANHLLHRC",
    "response#3": "LELHGALFSWDPVGTSLETFISHLEVKKGMLVGIVGKVGCGKSSLLAAIAGELHRLRGHVAVRGLSKGFGLATQEPWIQFATIRDNILFGKTFDAQLYKEVLEACALNDDLSILPAGDQTEVGEKGVTLSGGQRARIALARAVYQEKELYLLDDPLAAVDADVANHLLHRR"
  },
  {
    "reference": "MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINYEEFVKVMMAK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be able to bind Ca(2+) ligand in a variety of conditions2. The designed protein must possess calcium ion binding to support regulation of photomorphogenesis, defense response to fungus under certain conditions.3. The protein must exhibit the following characteristics: hordei, probably via its Ca(2+)-dependent interaction with ABCG36.; Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca(2+); Activates MPK8 in vitro. The designed protein sequence is ",
    "response#1": "KWYFEVMVDEVAPFLTAQATHLRVGWALSEGYSPYPGGGEGWGGNGVGDDLYSYGFDGLHLWTGHVARPVTSPGQHLLGPEDVVSCCLDLSVPSISFRINGCPK",
    "response#2": "PWIFGLSLLGLTPLAERVSFLTEQLAFYTGPTLGGLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIANIRREQRFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIVMLISYIAYLVFQLL",
    "response#3": "KWYFEVMVDEVAPFLTAQATHLRVGWALSEGYSPYPGGGEGWGGNGVGDDLYSYGFDGLHLWTGHVARPVTSPGQHLLGPEDVVSCCLDLSVPSISFRINGCPK"
  },
  {
    "reference": "MGGKWSKRGWPQVRERMQQTPPAAEGVGAVSKDLEKHGAITSTNINNADSVWLRAQEEEEEEVGFPVRPQVPLRPMTYKGALDLSFFLKEKGGLEGLIYSQRRKDILDLWVYNTQGFFPDWHNYTPGPGVRYPLTFGWCFKLVPVDPEKVEEANEGENNCLLHPMSLHGMDDTEGEVLMWKFDSALARRHLAREMHPDFYKDC",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in membrane that is critical for suppression by virus of host autophagy.2. The protein must exhibit the following characteristics: Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD..3. The protein design should include a Diacidic; necessary for CD4 internalization motif to ensure proper function. The designed protein sequence is ",
    "response#1": "GTIVRG",
    "response#2": "LMGHHHHHHX",
    "response#3": "MPGVSLPRV"
  },
  {
    "reference": "MLDPIENAIEDIRNGKMIIVVDDEDRENEGDFVTAASNITPEMVNFMSLHGRGLICVPLVEDRCEELELALMVNNNTALHETAFTVSVDLLGHGCTTGISAHDRAKTIRALVDPLTTPADLGRPGHIFPLKAKKGGVLRRAGHTEATIDLARLAGFAPAGVLVEIMNEDGSMARLPELKKIAEKFQLSLISIKDLIEYRLKRDSLIDEIVRVDMPTEFGHFQLVAFSEKNTTNEHLALIKGTWEKDEPVMVRVHSSCFTGDILGSLRCDCGDQLHAAMQMVEKEGRGAILYMNQEGRGIGLVNKLKAYKLQENGMDTVEANLHLGFPMDKRDYGIGAQMLRHLGITKLRLMSNNPKKRAGLLGYGLEIVEAIPIEMEPNAHNRKYLTTKRDKLGHEILKGL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The D-ribulose 5-phosphate, GTP, Zn(2+), Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation.3. The designed protein should have GTP cyclohydrolase II activity, manganese ion binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, magnesium ion binding, zinc ion binding to facilitate riboflavin biosynthetic process.4. The designed protein must have at least one GTP cyclohydrolase II domain.5. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..6. A protein that is capable of catalyzing reactions within the 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4, riboflavin biosynthesis, Cofactor biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "RMASARLPTRFGLFYLHVYEETLNGKQHLALVKGLVDGAKNVMVRVHSSCITGDTLFSLRCDCGQQLDAALKQISQCGKGVLVYLAQEGRGIGLLNKIRSYGLQDKGLDTVEANLALGFKADLREYGIGAQILADLGLSTIKILTNNPRKIVGIEGYGLKVVERVERVAVAKMPTEFGYFDSHTYTSKVDNRVHVALTVGIEDVGDGGCFPPIDRPVLTRVHSQCLTGDVFGSLRCDCGPQLHEAMRRVQAEGLGVVLYISQEGRGIGLANKLRAYALQDTGLDTIEANLHLGFRPDEREFGTGAQILHDLGIRQLRVLTNNPKKLSGLQGYGVEIVEQVRVASVRLPTQFGEFVAYGYESLVDGEQHLALVKGEVDGQENVLVRVHSECLTGDVFHSLRCDCGDQLQAALDAIEKEGRGVLLYMAQEGRGIGLLNKLRAYELQEQGRDTVEANLELGFAPDMRDYGIGAQILADLGLSSIRIMTNNPRKLVGLEGYGLTITERVRVASVRLPTQFGEFVAYGYESLVDGEQHLALVKGEVDGQENVLVRVHSECLTGDVFHSLRCDCGDQLQAALDAIEKEGRGVLLYMAQEGRGIGLLNKLRAYELQEQGRDTVEANLELGFAPDMRDYGIGAQILADLGLSSIRIMTNNPRKLVGLEGYGLTITERVL",
    "response#2": "RVAEARIPTAHGAFTAVGYQSIYDDVEHVALVRGDIGDGEDVLVRVHSECLTGDVFGSLRCDCGPQLDAALEMVDQEGRGVVLYMRGHEGRGIGLMHKLQAYQLQDSGRDTVDANLDLGLPADARDYGTGAQILVDLGIRSMRLLTNNPAKRVGLDGYGLTITERVPLVVAASGKRAVETRLPTEYGEFTAIGYRHDLDGQEHIALIAGDPAQLRGARDVVVRVHSECLTGDVFGSRRCDCGPQLQESLRKVQEAGSGVVIYLRGHEGRGIGLVHKLQAYRLQDEGWDTVDANLEQGLPEDAREYSVAGQILKDLGVESAQLLTNNPHKGEGLAGFGIEVSRHERVAEAALPTAYGEFRAITYRTLIDAKEHQAFVMGNVDTDDPVLARVHDQCVTGDVFGSLRCDCGEQLEEAIRRVASEHRGVIVYMDQEGRGIGLHNKIRAYRLQDSGMDTVEANEALGLPADGREYGIGMQILVDLGVRKLRLMTNNPTKRSGLEGFGLEIVERVERVAEAALPTAYGEFRAITYRTLIDAKEHQAFVMGNVDTDDPVLARVHDQCVTGDVFGSLRCDCGEQLEEAIRRVASEHRGVIVYMDQEGRGIGLHNKIRAYRLQDSGMDTVEANEALGLPADGREYGIGMQILVDLGVRKLRLMTNNPTKRSGLEGFGLEIVERVGVDLPTDFGDFELIAFRQTNTQELHLALIKGKWDKDEPILVRVHSSCMTGDIFGSCRCDCGAQLHGAMQMVQKEGKGVILYMNQEGRGIGLINKLKAYKLQEEGMDTVQANLALGLPSDSRDYGVGAQILRDLEVSKLRLISNNPQKRVGLLGYGLQIVEQV",
    "response#3": "RMASARLPTRFGLFYLHVYEETLNGKQHLALVKGLVDGAKNVMVRVHSSCITGDTLFSLRCDCGQQLDAALKQISQCGKGVLVYLAQEGRGIGLLNKIRSYGLQDKGLDTVEANLALGFKADLREYGIGAQILADLGLSTIKILTNNPRKIVGIEGYGLKVVERVERVAVAKMPTEFGYFDSHTYTSKVDNRVHVALTVGIEDVGDGGCFPPIDRPVLTRVHSQCLTGDVFGSLRCDCGPQLHEAMRRVQAEGLGVVLYISQEGRGIGLANKLRAYALQDTGLDTIEANLHLGFRPDEREFGTGAQILHDLGIRQLRVLTNNPKKLSGLQGYGVEIVEQVTDATPRYNRERVAVAKMPTEFGYFDSHTYTSKVDNRVHVALTVGIEDVGDGGCFPPIDRPVLTRVHSQCLTGDVFGSLRCDCGPQLHEAMRRVQAEGLGVVLYISQEGRGIGLANKLRAYALQDTGLDTIEANLHLGFRPDEREFGTGAQILHDLGIRQLRVLTNNPKKLSGLQGYGVEIVEQVTDATPRYNRMERVAVAKMPTEFGYFDSHTYTSKVDNRVHVALTVGIEDVGDGGCFPPIDRPVLTRVHSQCLTGDVFGSLRCDCGPQLHEAMRRVQAEGLGVVLYISQEGRGIGLANKLRAYALQDTGLDTIEANLHLGFRPDEREFGTGAQILHDLGIRQLRVLTNNPKKLSGLQGYGVEIVEQVTDATPRYNRM"
  },
  {
    "reference": "MHKKTLLATLILGLLAGQAVAAPYLPLASDHRNGEVQTASNAWLEVDLGAFEHNIQTLKDRLGDKGPKICAIMKADAYGHGIDLLVPSVVKAGIPCIGIASNEEARVAREKGFTGRLMRVRAATPAEVEQALPYKMEELIGSLVSAQGIADIAQRHHTNIPVHIALNSAGMSRNGIDLRLADSKEDALAMLKLKGITPVGIMTHFPVEEKEDVKMGLAQFKLDSQWLLEAGKLDRSKITIHAANSFATLEVPDAYFDMVRPGGLLYGDSIPSYTEYKRVMAFKTQVASVNHYPAGNTVGYDRTFTLKRDSWLANLPLGYSDGYRRALSNKAYVLIQGQKVPVVGKTSMNTIMVDVTDLKGVKPGDEVVLFGRQGEAEVKQADLEEYNGALLADMYTIWGYTNPKKIKR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be able to bind substrate ligand in a variety of conditions2. Include a signal peptide as an essential component of the protein.3. The intended protein structure should align with strand-strand-helix-strand-helix-helix-strand-helix-strand-helix-helix-strand-helix-strand-helix criteria.4. For general function, the protein need meet that Enables bacteria to produce and release extracellular non-canonical D-amino acids (NCDAAs) that regulate diverse cellular processes .; Reversibly racemizes ten of the 19 natural chiral amino acids known, including both non-beta-branched aliphatic amino acids (Ala, Leu, Met, Ser, Cys, Gln and Asn) and positively charged amino acids (His, Lys and Arg); Amino-acid racemase able to utilize a broad range of substrates The designed protein sequence is ",
    "response#1": "VALIAPASAIATDVLEATLRQLEVHGVDYHLGRHVEARYRYLAGTVEQRLEDLHNAFDMPDITAVWCLRGGYGCGQLLPGLDWGRLQAASPRPLIGFSDISVLLSAFHRHGLPAIHVALIAPASAIATDVLEATLRQLEVHGVDYHLGRHVEARYRYLAGTVEQRLEDLHNAFDMPDITAVWCLRGGYGCGQLLPGLDWGRLQAASPRPLIGFSDISVLLSAFHRHGLPAIHVALIAPASAIATDVLEATLRQLEVHGVDYHLGRHVEARYRYLAGTVEQRLEDLHNAFDMPDITAVWCLRGGYGCGQLLPGLDWGRLQAASPRPLIGFSDISVLLSAFHRHGLPAIHIAAQRMGEGALIGGNLTALACMAGTLGGLHAPAGSILVLEDVGEPYYRLERSLWQLLESIDARQLGAICLGSFTDCPRKEVAHSLERIFGEYAAAIEVPLYHHLPSGHGAQNRAWPYGKTAVALIAPASAIATDVLEATLRQLEVHGVDYHLGRHVEARYRYLAGTVEQRLEDLHNAFDMPDITAVWCLRGGYGCGQLLPGLDWGRLQAASPRPLIGFSDISVLLSAFHRHGLPAIHIAAQGAGAVASWS",
    "response#2": "VALIAPASAIATDVLEATLRQLEVHGVDYHLGRHVEARYRYLAGTVEQRLEDLHNAFDMPDITAVWCLRGGYGCGQLLPGLDWGRLQAASPRPLIGFSDISVLLSAFHRHGLPAIHVALIAPASAIATDVLEATLRQLEVHGVDYHLGRHVEARYRYLAGTVEQRLEDLHNAFDMPDITAVWCLRGGYGCGQLLPGLDWGRLQAASPRPLIGFSDISVLLSAFHRHGLPAIHVALIAPASAIATDVLEATLRQLEVHGVDYHLGRHVEARYRYLAGTVEQRLEDLHNAFDMPDITAVWCLRGGYGCGQLLPGLDWGRLQAASPRPLIGFSDISVLLSAFHRHGLPAIHTPARASVALIAPASAIATDVLEATLRQLEVHGVDYHLGRHVEARYRYLAGTVEQRLEDLHNAFDMPDITAVWCLRGGYGCGQLLPGLDWGRLQAASPRPLIGFSDISVLLSAFHRHGLPAIHTPARASAEAPTRAEGALIGGNLTALACMAGTLGGLHAPAGSILVLEDVGEPYYRLERSLWQLLESIDARQLGAICLGSFTDCPRKEVAHSLERIFGEYAAAIEVPLYHHLPSGHGAQNRAWPYGKTAGTLLSDMGIAR",
    "response#3": "VALIAPASAIATDVLEATLRQLEVHGVDYHLGRHVEARYRYLAGTVEQRLEDLHNAFDMPDITAVWCLRGGYGCGQLLPGLDWGRLQAASPRPLIGFSDISVLLSAFHRHGLPAIHLDEGSGAKLAVALRVALIAPASAIATDVLEATLRQLEVHGVDYHLGRHVEARYRYLAGTVEQRLEDLHNAFDMPDITAVWCLRGGYGCGQLLPGLDWGRLQAASPRPLIGFSDISVLLSAFHRHGLPAIHLDEGSGAKLAVALFG"
  },
  {
    "reference": "MLSARSAQCMVSMATRSCVSRGSAGSAAAGPVEAAIRAKLEQALSPEVLELRNESGGHAVPAGSETHFRVAVVSSRFEGMSPLQRHRLVHEALSEELAGPVHALAIQAKTPAQWRENPQLDISPPCLGGSKKTRGTS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The intended protein structure should align with strand-helix-strand-strand-strand-helix-helix-helix-turn-strand-turn criteria.2. The protein must exhibit the following characteristics: May protect cells against oxidative stress .. The designed protein sequence is ",
    "response#1": "LLYGPPGVGKTLLAKAVATESNANFISIKGPEVLSKWVGESEKAIREIFKKAKQVAPAIVFLDEIDSIAPRRGTTSDSGVTERIVNQLLTSLDGIEVMNGVVVIGATNRPDIMDPALLRAGRFDKLIYIM",
    "response#2": "PNRVLVDTGKTGSNITHTAKIISSTAGYRVPVTIERLKDGTFHVSFPAVPGKIPFVILMRALGILTDRDIVYAVSLDPEIQNELFPSLEQASSIANVDDALDFIGSRVAIGQKRENRIEKAQQIIDKYFLPHLGTSADDRRKKAYYLAYAISKVIELYLGRREPDDKDHYANKRLN",
    "response#3": "PLCKDPYGKEVQSMVENLLVQANVDTTNAFDNFKQPCPQLTKEQVAMCKGFDYGDKTLKLPCGPLPWPAGLPEPGYVPKTNPLHGRWITVSGGQVAFIKEAIKSGMLGAAEAKKIIADTDHHQTGGMYLRINQYGEVCTVDASVAKFARAKRTWKSGHYFYEPLVSGGNLLGVWVLPEEYRKIGFFWEMESGHCFRIERRR"
  },
  {
    "reference": "MAKYIFVTGGVVSSLGKGITAASIGCILKNCGLRVNILKIDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGNYERFMDVNLTQDNNFTTGRVYNTIITKERKGEFLGGTVQVIPHVTDEIKSRIKKIADNYDVVITEIGGTTGDIEGLPFLEAIRQFRLDLGWDNVCYIHVTLVPYIRAAEEMKTKPTQQSVAKLREIGIEPQIIICRTERPLTESMRKKIALFCNVQEKTVLEERDVSFSIYEVPVMLKKQGLDKLILEMLNLKGKSSNLSNWGKLLNKMKKCRKKITVAVAGKYTILKDAYKSIWEALTHAGVANGVSTEIKYVDVENKNLKNELKNADAILVPGGFGERGIEGKIEVVRYARENKIPFLGLCLGMQCAVIEFARNVCGMKGANSIEFDAKSKYPVIDMMLEQKKLTQKGATMRLGEYPCEIKNGSLAHRAYKKNLVFERHRHRYELNNEFRDILESKGLKITGECRGKKLAEIVEINNHPWFIAVQFHPEFKSRPNKPHPLFVNFIKAAK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein have CTP synthase N-terminal domains with specific sequence motifs that are crucial for its activity.2. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.3. The active site, Nucleophile active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.4. The protein should have a high affinity for ATP.5. The protein must have high specificity towards CTP synthase activity, metal ion binding. The designed protein sequence is ",
    "response#1": "LQDAYKSLREALVHGGIANKVKVNIEWIDSEIFESEDAVLHLEGVNGILVPGAFGKRGAEGKIKSITFARERLVPYFGICFGMQMAVVESARNLAGLPDASSTEFEKPCLPLVGLMTAWEHDGGQVTRSASSDMGGTLRLGAYDAHLTEGSQVQKIYGATTISERHRHRYEVNINYVEKLEETGMVFSGMSPDGVLPEIVEIPEHPWFIGVQFHPELKSRPLDPHPLFVSFIS",
    "response#2": "LPDAYLSVTEALRAGGFANWARVKIRWVASDSCETAEGAAEQLGDVDGICIPGGFGIRGVEGKIGAVRFAREKRIPILGLCLGLQVMVIEVARDMAGIEGADSSEFNPGAEHPVIATMAEQEAIVSGEGDLGGSMRLGSYEATLERDSVVAGLYGSVHVTERHRHRYEVNNTYRSQLEQAGLVVSGTSPDHHLVEFIELPQDVHPYFVATQAHPEFKSRPTRPHPLFAGLLGA",
    "response#3": "LPDAYLSVSEALRAGGFANNAKVSIIWIPSDDCETPEGAKKALGEVDAICVPGGFGVRGIEGKLGALKFARENKIPTLGLCLGLQCMVIEAARNLAGIKDANSAEFSPESGTHVIATMDDQKEIVSGQADMGGTMRLGLYTATLKPGSIVAKTYGAEEISERHRHRYEVNNQYRDQISSAGLTFSGMYKERDLVEFVELPSEVHPYYVGTQAHPELRSRPTRPHPLFIGLVESALASQ"
  },
  {
    "reference": "MQLNASEISDLIKQRIGKFDLGVESRSEGTIVSLTDGIVRIHGLQDVMQGEMVEFPGNTYGMALNLERDSVGAVVLGSYEHLSEGDTAKCTGRILEVPVGEALLGRVVDALGNPIDGAGPIDAKLFSPIEKIAPGVISRQSVSQPLQTGLKAIDSMIPVGRGQRELIIGDRQTGKTAIAIDAIINQKGTGVKCIYVAVGQKQSSIANVVRKLQEHGAMEHTIVVSASASESAALQFIAPYAGCAMGEYFRDRGEDALIVYDDLTKQAWAYRQISLLLRRPPGREAYPGDVFYLHSRLLERASRINAEEVERLTKGEVKGKTGSLTALPIIETQAGDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNAGLSVSRVGGAAQTKVIKKLGGGIRLDLAQYRELAAFAQFASDLDEATRKQIERGQRVTELMKQNQYSPMSIAQMAVSLFAANEGYLDDIEVSKVRDFEDALQEYMKSQCAELLKSIDATGDYNDAIQAGLHEAIKKFKATHAW",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein can be targeted to proton-transporting ATP synthase complex, catalytic core F(1) and perform proton-transporting ATPase activity, rotational mechanism, ATP binding.2. The protein should be able to bind ATP ligand in a variety of conditions3. For general function, the protein need meet that The alpha chain is a regulatory subunit. The designed protein sequence is ",
    "response#1": "GILAIDSMIPIGRGQRELIIGDRQTGKTAIAVDTIINQKDKGVYCIYVAIGQKASTVASVVNTLEKYGAMEYTTVVAATASESAALQYIAPYAGCAMGEHFMYQGKDVLVVYDDLSKHAVAYRTLSLLLRRPPGREAYPGDVFYLHSRLLERSARLSDEYGGGSLTALPIVETQAGDISAYIPTNVISITDGQIYLESELFYSGIRPAINVGLSVIRGGKTGLFGGAGVGKTVLIMELINNIAKQQSGYSVFAGVGERTREGNDLWEEMKESGVIDKTALVFGQMNEPPGARARVALTALSIAEYFRDEENRDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGALQERIVSTKKGSITSVQAIYVPADDLTDPAPATTFTHLDATTVLSRSIAELGIYPAVDPLDSTSRILDPNIIGRDHYDTAQAVKQISRMLSPHSLAFRLFGNNIIGAEHYNIARGVQKVLQDYKNLQDIIAILGMDELSEEDKLTVARARKIQRFLSQPFQVAEVFTGTPGKYVDLKDILVGEEHYETAQGVKQTLQRYKELQDIIAIPGLDELSEEDRLTVARARRIERFLSQPFFVAEVFTGSPGKYVSLRETIKGFQMILAGELD",
    "response#2": "RGGKTGLFGGAGVGKTVLIMELINNIAKQQSGYSVFAGVGERTREGNDLWEEMKESGVIDKTALVFGQMNEPPGARARVALTALSIAEYFRDEENRDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGALQERIVSTKKGSITSVQAIYVPADDLTDPAPATTFTHLDATTVLSRSIAELGIYPAVDPLDSTSRILDPNIIGRDHYDTAQAVKQIIAGMGAPSY",
    "response#3": "RGGKTGLFGGAGVGKTVLIMELINNIAKQQSGYSVFAGVGERTREGNDLWEEMKESGVIDKTALVFGQMNEPPGARARVALTALSIAEYFRDEENRDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGALQERIVSTKKGSITSVQAIYVPADDLTDPAPATTFTHLDATTVLSRSIAELGIYPAVDPLDSTSRILDPNIIGRDHYDTAQAVKQIS"
  },
  {
    "reference": "MDKFNPKKEKTVKKRALKVLTEISPTPLFSMLFLLHISQIATYLSLSDKETLNITVKTKQGGTDTCAGRYVYMHNLPSRFNEDLIKSCEAYIELRNKCKYLINSGFGPRILEEDHNHTTRVLTIETGSWYYTNQFMLEVIFREKMRHYECLTNDSSLSSVVFVPFYAGFDVRRFWGYNVKLRDELGEDLAQWLRERPEWRKMYGRDHFFVTGRVGRDFRRVTDQDSDWGNKLMRLPEFENITMLSIETNSRSNEFAVPYPTYFHPKSRTEVKRWQRQVTMMQRRYLFSFVGANRPKMEESIRGEIIRQCLASQGRCKFLDCDTSSKDCSDPVKVVEVFQDSVFCLQPPGDTPTRRSTFDSILAGCIPVFFSVDSVYNQYKWYFPKDRTKYSVYIAEEGVKKGKVSIEKLLANVSEEKISRMRNEVEKIIPKIIYTKPGEVGPEKIEDAFEIAVARVLERVSLFKMTRI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Functions in xyloglucan synthesis by adding side chains to the xylosylated glucan backbone.2. The protein must be able to glycosyltransferase activity. The designed protein sequence is ",
    "response#1": "MSPFKIFFFTTLLVAAFSVSAADFNTDVNVAWGNGRGKILNNGQLLTLSLDKSSGSGFQSKTEYLFGKIDMQIKLVPGNSAGTVTTFYLKSEGSTWDEIDFEFLGNMSGDPYTLHTNVYTQGKGDKEQQFHLWFDPTANFHTYSILWNPQRIILTVDDTPIREFKNYESLGVLFPKNKPMRMYASLWNADDWATRGGLVKTDWSKAPFMASCN",
    "response#2": "DDNYVVTWGQNNVLKLNQGKEVQLSLDHSSGSGFESKNHYESGFFQIRIKVPPKDTSGVVTAFYLTSKGNTHDEVDFEFLGNKEGKLAVQTNVFTNGKGNREQKLALWFDPSKDFHTYAILWNPYQIVLYVDNIPVRVFKNTTSQGMNYPSKPMQVVVSLWNGENWATDGGKSKINWC",
    "response#3": "MSPFKIFFFTTLLVAAFSVSAADFNTDVNVAWGNGRGKILNNGQLLTLSLDKSSGSGFQSKTEYLFGKIDMQIKLVPGNSAGTVTTFYLKSEGSTWDEIDFEFLGNMSGDPYTLHTNVYTQGKGDKEQQFHLWFDPTANFHTYSILWNPQRIILTVDDTPIREFKNYESLGVLFPKNKPMRMYASLWNADDWATRGGLVKTDWSKAPFMASC"
  },
  {
    "reference": "MNMRVADVARIAGGTVHGDGDVMVHGIGLDSQELAEGALFAALPGTRNHGARFAADTQAAAILSDAQGLELLQQAGEQRPVIVVDDIRTILGSVSSALYDNPSQKLCVIGITGTSGKTTTSYLLEAGLMHAGLHVGLIGTTGTRINGVKVPTQLTTPEAPKLQELFARMVEQGVTHVVMEVSSHALALGRVGGTQFDAAGFSNLTQDHLDFHPTMEDYFETKAKLFDPSSELSPEKSVICIDDEWGQAMFGRAKHPWALASKSSQEGEIVANYFVEDAQSDDAGTQRFVLHARDKRIPVDLNMPGAFNITNASLAIALADAVGVDATVVAEGVAGVGVPGRMQRIDAGQDFIAVVDYAHKPAAVAAVLDTIRAQTPGRVAVVLGAGGNRDVSKRALMGEAAASRADLLIVSDDNPRDEDPSQIRQAIIEGAQGVEDGAQVEEEGDRLEAIRKAVRWAQAGDAIVVAGKGHEVGQLIRGVQHHFDDREALETAIHEALNTQEHSAEHSDEPSADPSAQAGDNA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that contributes to cell wall organization, cell cycle by functioning in cytoplasm with ATP binding.2. The protein should be able to bind UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP ligand in a variety of conditions3. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..4. The presence of Mg(2+) is necessary for the protein to function in the desired environment.5. The protein design should take into account the physiological conditions of the peptidoglycan biosynthesis, Cell wall biogenesis pathway. The designed protein sequence is ",
    "response#1": "MELKHLRYFLTVAEELHFGRAAARLHMTQPPLSRQIQDLEHEVGATLFERTTRSVRLTPAGEAFLPHARRVLAMVDEAVRVTRQAGSGELGLLRIGFVGSAMFSGVPKIVRRFRAAYPDVRLELMEMGTSEQIAGLRDGRIDLGFIRPPFDLAGLDSVAVHDEPLVAAVPADHPLAGRDRLALADLADEPFISFPRRSGPGLYDQIMALCGQAGFTPRISQEATQMQTLVSLVAAGFGIALVPASARAMRLPGVRFIEIDDAPASTELALAWRRGDENPCLRAFIELAREVSAAG",
    "response#2": "MKLVTGTNGKTTTTHLIEFLLQEAGKSPALFGTLYARWQGFEQVATHTTPFSVHLQEKLAQARNAGAEVGVMEVSSHALAQSRVLGCEFEVAAFTNLTQDHLDYHENMDEYFAAKALLFSPQYLKGRAVVNVGDAYGQLLADQIPGDRLWTYACEDTSADLYTENLTYTPTGVSGTIQSPQGSAAFSLPLVGQYNLENMLAAVGLAIEQGFDLDLIVDVLPQFSGVPHRFQMAFERNGVRWINDSKATNVGSTEAALSGLAVDGTLHLLLGGDGKSADFSPLVPYLQGERIRLYCFGRDGQQLAALRPEIAEQTETMEQAMRVIAERIRPGDMVLLSPALFEEWSRR",
    "response#3": "MKLITGTNGKTTTAYLVAALFEAADIRCGVLGTVVYKVGHEVREATRTTPEPPDVQMLLREMVTAGCGACAMEVSSHALSLNRVDGMSFSAAVFTNLTRDHLDFHGDMETYFQAKRRLFDMTAADAPSLINIDDPRGAGLAESVSRPVTYAINRNADIMPGPLSFSLSGVAFDVRTPRGPVHVHSPLVGRPNVYNILAAVSTGLALGFDFETIIKGLEAFPGVKHRLQYVTTLDGVKVYNDSKSTNIEAATVAIQSFKQPEVLLAGGLDRGFVFDSLVDLFKKHVKAIVTYGETRYLLADAARKAGIKTIVVVDNLHEGVKAASKLAEAGDVLLFSPAHRGQCVGRHREVVAIDNSKATNPHAADSALAGFSSVVWVAGGQLKGAEIDELIVRHAGRIKAVALLGVDRDVIEDSVRTHIPGIPVLSVSETDPRRAMDEAVAWSVSQAEAGDAIVLAPGDITRVGPELVRLLGPS"
  },
  {
    "reference": "MKKIPRIGVGGPVGSGKMAIIEAVVPILIKLGYRILVITNDIVTTEDAKHVQRTLKGVLIEDRIVGVETGGCPHTAVREDPSMNLAAVEEMEAKFPDTDLVLLESGGDNLTLTFSPALIDFFIYVIDVAAGDKIPCKNGPGISQSDILVINKTDLAPYVGASLQVMDDDSRMMRGKKPFVFTNCKTNEGIDDLVHLIRENVLFDTEVSKESA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that can perform nickel cation binding in cytoplasm to support nitrogen compound metabolic process.2. The protein should be able to bind GTP ligand in a variety of conditions3. The protein must exhibit the following characteristics: Disrupting the ure2 operon has no effect on urease activity, or pathogen survival in BALB/c mice when inoculated by gavage, but confers slightly enhanced resistance to low pH killing in vitro.. The designed protein sequence is ",
    "response#1": "ILTAAGIDTHIHLISPQQAYHALSNGVTTFFGGGIGPTDGSNGTTVTAGPWNIRQMLRSFESLPLNVGLLGKGNACGYGPLEEQIIAGAAGLKVHEDWGATASALRYALRIADKMDIQVAVHTDSLNEGGYVEDTIDAFEGRTVHTFHTEGAGGGHAPDIIKVASLMNVLPSSTNPTLPFGINSQAELFDMIMICHNLNPNVPADVAFAESRVRPETIAAENVLQDMGVISMFSSDSQAMGRVGENWLRLIQTANAMKVARGKLPEDAAGNDNFRVLRYVAKITINPAIAQGVSHVIGSIEVGKMADLVLWDPRFFGAKPKMVIKGGMIAADYCAWPALSFE",
    "response#2": "VGIGGPVGSGKTALVEALCKALRDHYEIAVVTNDIYTREDAEFLTRNGALPADRIIGVETGGCPHTAIREDASMNLAAVGDLNQRFPELEVVFIESGGDNLSATFSPELSDLTLYVIDVCAGDKIPRKGGPGITRSDLLVINKIDLAAGVGASLEVMDRDSRRMRGDRPFI",
    "response#3": "ILTAAGIDTHIHLISPQQAYHALSNGVTTFFGGGIGPTDGSNGTTVTAGPWNIRQMLRSFESLPLNVGLLGKGNACGYGPLEEQIIAGAAGLKVHEDWGATASALRYALRIADKMDIQVAVHTDSLNEGGYVEDTIDAFEGRTVHTFHTEGAGGGHAPDIIKVASLMNVLPSSTNPTLPFGINSQAELFDMIMICHNLNPNVPADVAFAESRVRPETIAAENVLQDMGVISMFSSDSQAMGRVGENWLRLIQTANAMKVARGKLPEDAAGNDNFRVLRYVAKITINPAIAQGVSHVIGSIEVGKMADLVLWDPRFFGAKPKMVIKGGMIYAADYCAYPVSSAEATVDKTIPAACGIK"
  },
  {
    "reference": "MAACTAAQQLLLVLSALGLLAAGAPQPPNIVLLLMDDMGWGDLGVNGEPSRETPNLDRMAAEGMLFPSFYSANPLCSPSRAALLTGRLPIRNGFYTTNAHARNAYTPQEIMGGIPNSEHLLPELLKKAGYTNKIVGKWHLGHRPQFHPLKHGFDEWFGSPNCHFGPYDNKAKPNIPVYRDWEMVGRFYEEFPINRKTGEANLTQLYTQEALDFIQTQHARQSPFFLYWAIDATHAPVYASRQFLGTSLRGRYGDAVREIDDSVGKILSLLQNLGISKNTFVFFTSDNGAALISAPNEGGSNGPFLCGKQTTFEGGMREPAIAWWPGHIAAGQVSHQLGSIMDLFTTSLSLAGLKPPSDRVIDGLDLLPTMLKGQMMDRPIFYYRGNTLMAVTLGQYKAHLWTWTNSWEEFTQGTDFCPGQNVSGVTTHTQEEHTELPLIFHLGRDPGERFPLSFHSDEYQDALSRTTQVVQEHQKSLVPGQPQLNVCNQAVMNWAPPGCEKLGKCLTPPESVPEKCFWAH",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be able to bind Ca(2+) ligand in a variety of conditions2. A protein with sulfuric ester hydrolase activity is needed to enhance chondroitin sulfate catabolic process in a specific context.3. The protein design should incorporate a binding pocket that specifically recognizes Ca(2+) to enhance enzymatic efficiency.4. The protein must contain a signal peptide for proper functionality.5. The protein with the reaction Hydrolysis of the 6-sulfate groups of the N-acetyl-D-   galactosamine 6-sulfate units of chondroitin sulfate and of the D-   galactose 6-sulfate units of keratan sulfate., the Nucleophile must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "QSWLAIARGFSRYLVGNESYENNNRYGRYLQYGQLEIIPADLTQSGFSHAGWDWNRYPGTTTIHLPYNELEAKLNQLPAAGIEEMLLSTESYSGANTLNNNSMFAMKLHGHSKYQQQSLRANKSYFLFDNRVIALGSGIENDDKQHTTETTLFQR",
    "response#2": "DDQTEMASIYQRFEYYQGIRSDKKITPDMLDKHLALWEKLVLTQHADGSITGKALDHPNRQHFMKVEGVFSEGTQKALLDANMLRDVGKTLLQTAIYLRSDSLSATDRKKLEERYLLGTRYVLEQGFTRGSGYQIITHVGYQTRELFDAWFIGRHVLAKNNLLAPTQQAMMWYNATGRIFEKNNEIVDANVDILNTQLQWMIKSLLMLPDYQQRQQALAQLQSWLNKTILSSKGVAGGFKSDGSIFHHSQHYPAYAKDAFGGLAPSVYALSDSPFRLSTSAHERLKDVLLKMRIYTKETQIPVVLSGRHPTGLHKIGIAPFKWMALAGTPDGKQKLDTTLSAAYAKLDNV",
    "response#3": "VFSLLNLDYPGLEKVKALHQEGKDADAAKALLDYYRARTNVKTPDINLKKVTIGKDEQKMADEALQHTFFAHKGYQPSFNYGEDIDWRYWPVKDNELRWQLHRHKWFTPMGKAYRVSGDEKYAVEWTKQYIDWIKKNPLVKVDKKEYEMTGDNQLKGDVENARFAWRPLEVSNRLQDQTSQFQLFLPSPSFTPEFLTEFLVNYHKHAIHILGIYSAQGNHLLFEAQRMIYAGAFFPEFKEAAAWRKSGIDIMNREINVQVYNDGGQFELDPHYHLAAINIFCKALNIADLYGFRNEFPQEYLDTIEKMIVFYANVSFPDYTNPCFSDAKLTNKKEMLKNYRNWSKMFPKNQFIKPQAIIIGVKKGGTRALLEFLRVHPDVRAVGAEPHFFDRSYDKGLAWYRDLMPRTLDGQITMEKTPSYFVTREAPARISAMSKDTKLIVVVRDPVTRAISDYTQTLSKRPDIPTFESLTFKNRTAGLIDTSWSAIQIGIYAKHLEHWLRHFPIRQMLFVSGERLISDPAGELGRVQDFLGLKRIITDKHFYFNKTKGFPCLKKAEGSSRPHCLGKTKGRTHPEIDREVVRRLREFYDDQTEMASIYQRFEYYQGIRSDKKITPDMLDKHLALWEKLVLTQHADGSITGKALDHPNRQHFMKVEGVFSEGTQKALLDANMLRDVGKTLLQTAIYLRSDSLSATDRKKLEERYLLGTRYVLEQGFTRGSGYQIITHVGYQTRELFDAWFIGRHVLAKNNLLAPTQQAMMWYNATGRIFEKNNEIVDANVDILNTQLQWMIKSLLMLPDYQQRQQALAQLQSWLNKTILSSKGVAGGFKSDGSIFHHSQHYPAYAKDAFGGLAPSVYALSDSPFRLSTSAHERLKDVLLKMRIYTKETQIPVVLSGRHPTGLHKIGIAPFKWMALAGTPDGKQKLDTTLSAAYAKLDN"
  },
  {
    "reference": "MNFRFGIWFAVPLILCGITPEVPASWWYMGALGSSRVMCDNVPGLVSRQRQLCQRHPEAMRSIGLGVAEWAAECQHQFRQHRWNCNTLHRHLLFLFLLVSGSRESAFVYAISSAGVVFAITRACSQGELKSCSCDSKKKGSAKDNRGQFDWGGCSDNVDYGVKFARAFVDAKERKGKDARALMNIHNNRAGRKAVKRFLKHECKCHGVSGSCTLRTCWLAMGDFRKTGDYLWRKYNGAIQVVMNQDGTGFTVANKRFKKPTNNDLVYFESSPDYCIRDWDVGSLGTAGRICNQTSRGMDSCEVMCCGRGYDTSRISRMTKCECKFHWCCAVRCQDCLEVVDVHTCKAPKNAAWITRT",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be involved in lung induction, cardiac epithelial to mesenchymal transition, positive regulation of cardiac muscle cell proliferation, cell proliferation in midbrain, positive regulation of DNA-binding transcription factor activity by localizing to collagen-containing extracellular matrix, cytoplasm, extracellular region, extrinsic component of external side of plasma membrane and exhibiting frizzled binding, cytokine activity.2. Incorporate a signal peptide in the protein design.3. The protein must exhibit the following characteristics: Ligand for members of the frizzled family of seven transmembrane receptors.. The designed protein sequence is ",
    "response#1": "MFFSHHHTRFARLWILTWSVLCCASTFFTVTTSLVAMQRFRYPERPIIFLSGCYTMVSVAYIAGFVLQERVVCNERFSEDGYRTVGQGTKKEGCTILFMMLYFFSMASSIWWVILSLTWFLAAGMKWGHAAIEANSQYFHLAAWAVPAVKTITILAMGQIDGDLLSGVCFVGLNRLDPLRGFVLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEPLERLMVRIGVFSVLYTVPATIVIACYFYEQAFREHWERSWVSQHCKSLAIPCPAHYTPRTSPDFTVYMIKYLMTLIVGITSGFWIWSGKTLHSWRKFYTRLTNARS",
    "response#2": "FFSHHHTRFARLWILTWSVLCCASTFFTVTTSLVAMQRFRYPERPIIFLSGCYTMVSVAYIAGFVLQERVVCNERFSEDGYRTVGQGTKKEGCTILFMMLYFFSMASSIWWVILSLTWFLAAGMKWGHAAIEANSQYFHLAAWAVPAVKTITILAMGQIDGDLLSGVCFVGLNRLDPLRGFVLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEPLERLMVRIGVFSVLYTVPATIVIACYFYEQAFREHWERSWVSQHCKSLAIPCPAHYTPRTSPDFTVYMIKYLMTLIVGITSGFWIWSGKTLHSWRKFYTYTKPPQCVDIPADLRLCHNVGYKKMVLPNLLEHETMAEVKQQASSWVPLLNKNCHAGTQVFLCSLFAPVCLDRPIYPCRWLCEAVRDSCEPVMQFFGFYWPEMLKCDKFPEGDVCIAMTPPNACG",
    "response#3": "FFSHHHTRFARLWILTWSVLCCASTFFTVTTSLVAMQRFRYPERPIIFLSGCYTMVSVAYIAGFVLQERVVCNERFSEDGYRTVGQGTKKEGCTILFMMLYFFSMASSIWWVILSLTWFLAAGMKWGHAAIEANSQYFHLAAWAVPAVKTITILAMGQIDGDLLSGVCFVGLNRLDPLRGFVLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEPLERLMVRIGVFSVLYTVPATIVIACYFYEQAFREHWERSWVSQHCKSLAIPCPAHYTPRTSPDFTVYMIKYLMTLIVGITSGFWIWSGKTLHSWRKFYTGGQSS"
  },
  {
    "reference": "MLYPLLRPLLFKFDAETAHEHTLKMLDRAHRLHLTPLAASPAARQPVQAMGLTFPNPVGLAAGLDKNGAHIDALAALGFGFIEIGTVTPRPQDGNPKPRLFRLPEHEAIINRMGFNNHGVAALLDNVRRSKFKGVLGINIGKNAITPIENAVDDYLACLDQVYAAASYVTVNISSPNTKNLRQLQQGDELGRLLAALKQRQLALADQHGRYVPLAVKIAPDLDDEQIAEIARLLTGNGIDGVIATNTTLSRREVAGHPLESEAGGLSGAPVRARSTEVIRKLHKELGGAMPIIGVGGILSGNDAVEKLDAGASLVQLYSGLIYRGPELVGECARATAQYLQARN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should be able to enhance the efficiency of 'de novo' pyrimidine nucleobase biosynthetic process.2. We require a protein that can specifically bind to the metabolites produced in the UMP biosynthesis via de novo pathway pathway. The designed protein sequence is ",
    "response#1": "PFCLGIDPSRDLLTRWGLPDNARGLRDFCERVADAAGSSVAVVKPQSAFFERHGPEGLQVLQELMRRFKSVGTLTLLDVKRGDIGSTMEAYAETVFGEGSAYEADAATFTAYLGLGALLKTLERARASGAAAFLVVRSSNPEGTSLQMSRGEDGRTVAEALADGLRAFNEKPGQDAAPVAGAVMGATLPDSDRGVIERLGGALLLTPGIGAQGAGFDDLKRLFAGREAQVIPTATRSVLEAGPDTAALRQR",
    "response#2": "PFCLGIDPSRDLLTRWGLPDNARGLRDFCERVADAAGSSVAVVKPQSAFFERHGPEGLQVLQELMRRFKSVGTLTLLDVKRGDIGSTMEAYAETVFGEGSAYEADAATFTAYLGLGALLKTLERARASGAAAFLVVRSSNPEGTSLQMSRGEDGRTVAEALADGLRAFNEKPGQDAAPVAGAVMGATLPDSDRGVIERLGGALLLTPGIGAQGAGFDDLKRLFAGREAQVIPTATRSVLEAGPDTAALRA",
    "response#3": "PFCLGIDPSRDLLTRWGLPDNARGLRDFCERVADAAGSSVAVVKPQSAFFERHGPEGLQVLQELMRRFKSVGTLTLLDVKRGDIGSTMEAYAETVFGEGSAYEADAATFTAYLGLGALLKTLERARASGAAAFLVVRSSNPEGTSLQMSRGEDGRTVAEALADGLRAFNEKPGQDAAPVAGAVMGATLPDSDRGVIERLGGALLLTPGIGAQGAGFDDLKRLFAGREAQVIPTATRSVLEAGPDTAALRQL"
  },
  {
    "reference": "MRFKILQKDGLARTGIIETQRGIIHTPAFMPVGTNGTVKAMTPEEVRQIGYEIILSNTYHLYLRPGHETIKMIGGIHKFINWHCPILTDSGGFQIYSLASLRKITLEGVEFRSHIDGSLHFINPEKAIDIQLALGSDIMMVLDECIPYPADEEYVEKSIKLTTEWAKRCKEHFEKQDTYQALFGIIQGGVFSDLRIRALEELLKIDFHGYAIGGLSVGEPKTDMYKIVKDISPLMPEDKPHYLMGVGDLIDVLHAVEHGIDMFDCVIPTRNARNGTLFTSQGRISIKRSEFKEDLSPLDPDCDCYTCKNYSRAFLRHLYTCREILSMRLNTIHNLYFYCRFFEKMRQAIAERRFQEFKKEWLPVLEKNFYQESNDFHHSLENY",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that can modulate the overall flux of metabolites through the tRNA modification, tRNA-queuosine biosynthesis pathway, enhancing or suppressing its activity as needed.2. A protein that contributes to tRNA-guanine transglycosylation, tRNA wobble guanine modification, queuosine biosynthetic process by functioning in cytosol, cytoplasm with queuine tRNA-ribosyltransferase activity, metal ion binding.3. For general function, the protein need meet that The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product; The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate; Catalysis occurs through a double-displacement mechanism; After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1- yl)amino)methyl)-7-deazaguanosine).4. The substrate, Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.5. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "FEVIQKSGGSRARLGRLLTPHGVVETPCFMPVGTSATVKAIFPKDLEEEGIKLILANAYHLLFRPGISVIKEFGGLHQFMGWNGAILTDSGGFQVFSLSPFCKIFPEGVRFKSPVDGSFLMLTPESAIHAQMELGSDIVMSLDHCPPWPSKEKDLLEATRRTIQWAKRGKETWMQQQEKLFSKPSASYSLFGIIQGGTDERLRLYCSEELMKIGFDGYAIGGLSVGEPHEESLAVVKTVSSFLDEKAPKYVMGMGQPWQIVQMVDLGIDLFDCVLPTRLARHGSAYVEEGIIHIKNARFRKDGSPLDSRCSCYACRKFSRGYIHHLLKSKEILGIMLLSMHNLLFYNRLMKEIRSFLAHGQWVDFLAYPQEAHWQKEIIENARLGILETPHGVIETPVFMPVGTQATVKAMTPEELKEIGATIILSNTYHLYLRPGHKIIEKAGGLHRFMNWDRAILTDSGGFQIFSLSSLRKIKEEGVEFRSHIDGSKHFFTPEKVIEIQNALGSDIIMSFDECAPYPADYEYVKNSMELTIKWAERGKKAHKNTEKQALFGIVQGGIYEDLRKECAQRLVEMDFPGYSIGGLSVGEPKNVMYDIVDLTTEYLPENKPRYLMGVGSPDDLIEGVIRGVDMFDCVLPTRIARNGTVFTSKGKLIVRDAPYAEDFSPLDEECDCYTCKNYSRAYIRHLFKANEILAARLATIHNLYFLIKLMERIREAIRQDRLLEFKKQFFKKLKAK",
    "response#2": "GEARAGTFATPHGPVYTPCFMPVGTQATVKTLTPAQLAETGAQMILANTYHLSLQPGADIVAGAGGLHGFMQWPGPILTDSGGFQVFSLSSLRTIDDDGVTFREPKSGALVRFTPEHAVAVQNALGADVIMAFDECPPYPADREQVEGAVERTLRWFERCVEAHRRSDQALFGIVQGGVWPDLRRRCAEGLVAADLPGYAIGGVSVGEPQTLIERVVRVTAPLLPEHKPRYLMGVGTFREMAQAVAVGVDLFDCVMPTRVARHGSALLLGTGGDRRINLKNAQFRRDYEPLDCVCPCYTCRHFSRAYLAHLVRSEEILAMTLLSIHNVATLTRFAALLRCAIATGSFAQEFAHYLFDWLDGNPLAEDGHARLGRLICNQGIVDTPAFMPVGTYGSIKTLTAKEVEASGTQIILSNTFHLWLRPGLDIIKLHGSLHKFMSWNGPIITDSGGFQVFSLSKTRTITRKGVGFKSPIDGHLVFLTPEKSIEIQHDLQSDIVMIFDECISYPNTWDYVKKSVNISLNWAERSRLRFDTLHNSNMLFAIIQGGMYENLRDMSAKELINIGFDGYAIGGLSVGEPKKEMYRILSHICKLIPTNKPRYLMGAGKPEDLLEAVQKGIDMFDCVIPTRNARNGYLFVSDGTIKIRNAQYKKDTSPLDGNCDCYTCQHYSRSYLHHLDCCKEILGVRLNTIHNLRYYQRLMEELRQAIKTKSLQNFIDIFYKRFAPRLALQSG",
    "response#3": "ARRGRLTLAHGTVDTPAFMPVGTYGTVKAMTPAALADTGAQICLGNTFHLWLRPGLDVVAAHGGLHRFMNWDRPILTDSGGFQVFSLGALRKISEEGVKFASPIDGAKLFLTPEESMRIQTVLNSDIAMIFDECTPHPATHDEAAKSMQLSLRWARRSRDEFDRLANPNALFGIVQGGMFEDLRDESLAALDAIGFSGYAIGGLSVGEPKEDMARILAHTAPRLPAGRPRYLMGVGTPEDIVAGVAAGIDMFDCVMPTRNARNGWLFTRYGDIKIKNATHKQDTRPLDPSCDCYTCRNFSRAYLHHLHRAGEILGSMLNTIHNLRYYQTLTAELRAAIAAGDLDSHAARFRADARRGRLTLAHGTVDTPAFMPVGTYGTVKAMTPAALADTGAQICLGNTFHLWLRPGLDVVAAHGGLHRFMNWDRPILTDSGGFQVFSLGALRKISEEGVKFASPIDGAKLFLTPEESMRIQTVLNSDIAMIFDECTPHPATHDEAAKSMQLSLRWARRSRDEFDRLANPNALFGIVQGGMFEDLRDESLAALDAIGFSGYAIGGLSVGEPKEDMARILAHTAPRLPAGRPRYLMGVGTPEDIVAGVAAGIDMFDCVMPTRNARNGWLFTRYGDIKIKNATHKQDTRPLDPSCDCYTCRNFSRAYLHHLHRAGEILGSMLNTIHNLRYYQTLTAELRAAIAAGDLDSHAARFRADLERRAAA"
  },
  {
    "reference": "MKVATPEQIGRMDTEAITKIGIPGIVLMENAALKVVEEIVKSVGNLKEKKAVVLAGKGNNGGDAFAVARHLHNIGAKVLVFITSKKSEIKNDAEINLKIIERINIETVEIISENEVLKFKEELEACDVIIDGIFGTGFKGQISGIIDEVVEFVNSSSKYVVAIDIPSGVNGETGKVLGRCIKAHKTITFSYPKIGLLINPGCEYTGELVIADIGIPKIISDAVNMETTLIDESHVSGIIPERCRESSKGNYGRVLIVSGSTGMTGSGCLCAKAALRSGSGLVYLGVPSSLRDIYGTQLVEPIVIPLEDKGKGYLSKESINEIIKEMERMDAAAIGPGVSSRQDISDLMGELIISATIPLVLDADALNAVSKDVGVLRKSKVPVIVTPHPGEMSRLTGISIADIQKDRIKHARDFSGKYNVITVLKGAKTIIATPDGRIYINPTGNPGMSTAGTGDVLTGIITGFLGQGFASEDAAAAGVFLHGLAGDIAAAKKGEYGLIAGDLIDELPHAINKILQKKG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The (6S)-NADPHX binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must be able to ADP-dependent NAD(P)H-hydrate dehydratase activity. The designed protein sequence is ",
    "response#1": "VLLVAGSLGKSGAAILSGRGALRTGAGLVTIATPDIVLPHVAGGQPEYMSESLESTDEGTVAFSSLEGNSFEEILKGKTVLGIGPGLGTHTETQKFIRTVVRDTPLPTILDADGLNAFAGSGDLFRDRQSSFLALTPHPGEMGRLLGISTQTIQADRLAMALDAARRWNAHFVLKGHHSIVASPDGQVFVSTAGNAGLAKGGTGDVLTGILAALLAQFGANDGARVLSLGVYLHGLAAELASHGTDLSGLLAGEVADAVL",
    "response#2": "VLVIAGTKNMSGACYLSAMAAYRTGAGLVKVLTVEDNRSVIQTKLPEAIVITYEPTNLKSKIEIDRIVREIEWATAIVIGPGIGISAASDCLLDLVLKNAKIPVIIDADGINMLAHHSEYVFEDDQGKRKIDLPGNVILTPHLKEMSRLLDCDTAYVKDNLLSIAQSVTNNKEFVLALKDARTLVSNGKELYVNVSGNNGMSTGGSGDVLTGILAALVAQGLTPNEAACIGVYIHGRAGDHAREQVGTYSLMASDIINAIN",
    "response#3": "EENDVRRFLLPRGDFAHKGSVGNALLIAGSYGMAGASVLAARACLRSGVGRVTIHTPRRNYDIMQTAVPEAVMQMDREETYFSEAVDSEDYDAVGIGPGLGVAETTAIALIAQLRRSQVPIVVDADALNILANHRAWMQQLPKGMIMTPHPKEFDRLNGSPCSDDYERLIKARDMAQNLQLYILLKGHYSALCLPNGEVVFNSTGNSGMATAGSGDVLTGMITALLARGYKQKEACELGMYLHGLAGDLAARDLGKESLIASDIIAYLPKAFKRR"
  },
  {
    "reference": "MKDGGESKEQQLTVALRIRPINETELAEGATIIAHKVDKQMVVLMDPMEDSDDILRANRSREKSYMFDVAFDYTATQDTVYRFTTKGLIEGVISGYNATVFAYGPTGCGKTYTMLGTDWEPGIYIRTLNDLFKAIEETSDDMEYEVLMSYMEIYNEMIRDLLNPSLGYLDLREDSKGVIQVAGITEVSTINAKEIMQLLMKGNRQRTQEPTAANKTSSRSHAILQVTVRQKSRVKNITQEVRVGRLFMIDLAGSERASQTQNRGLRMKEGAHINRSLLALGNCINALSERGSNKYVNYRDSKLTRLLKDSLGGNSRTVMIAHISPASTSFEESRNTLTYADRAKNIKTRVKRNLLNVSYHIAQYTSIISDLRKEIQRLKKKIDEQGLKQIRSEKSDIRNIQAEVQLHSSTYGRHEMEQLKEQLIRAFREQMDIRRQLMEIENSSMEMQMETSRHFLITAEWEQEKTRRARKWRDEHRKETYGKDDSEKDSDTGDDQSDFIEPPEVITARETIQILEGDQNKLRRQKLELEKRFRDVRHHARRLEEALPKRISSDDQREILSLLCKVHELEIENTEMQSHALLKDNMIRQKDYMVQRFEQHRSLCDEIIQQQRRIIYDHNLTVPHQLNDLYELYFRELEEGVLDKAAVLALKDSQSCLPKIPNLTAEENLQEPDSDQESVRTFGSDNRNPIHRDSYKNLLPQILSETDSDTNKVFKTSPRARHLKNGAVVTPPPIHVNGIISKEYLPRNQINYFPDSTDSKVVLTHREK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a binding site that is specific to the target ATP ligand.2. The protein contains novel Kinesin motor domains that confer a unique function or activity.3. Ensure the presence of a signal peptide within the protein.4. The designed protein should have ATP binding within cilium to regulate axonemal microtubule depolymerization. The designed protein sequence is ",
    "response#1": "VGKVKVMLRVADRDRNSGGTEPDFMALDKKKRQVTLTDPRTACPPPQAAQERAPMVAAPKMFAFDNLFTGEDKQSDVCASALSEVIPAVLEGSDGCLLAMGYPATGQAQTVLGELGGGSGSGSASGSGVACSLGAAPCAIAWLYKGIQERRQKSGARFSVRVSAVGVSATKPDALSQDLLISHAAESDDSPGIYLRDDFLGGPTELRAPTAERAALFLDSALAGRLKSSGSTASGSSGCAAPLESALIFTLHVYQYSLSRKGGVAGGRSRLHIIDLGGCANRNGGLPLSGIGNILLAILSGQRHPPHKDHPLTPLLKDCLAPITCHVAIVAHVRPEQSYQDALSTIQIASRIHRLRRRKHRVR",
    "response#2": "KVKVMLRVADRDRNSGGTEPDFMALDKKKRQVTLTDPRTACPPPQAAQERAPMVAAPKMFAFDNLFTGEDKQSDVCASALSEVIPAVLEGSDGCLLAMGYPATGQAQTVLGELGGGSGSGSASGSGVACSLGAAPCAIAWLYKGIQERRQKSGARFSVRVSAVGVSATKPDALSQDLLISHAAESDDSPGIYLRDDFLGGPTELRAPTAERAALFLDSALAGRLKSSGSTASGSSGCAAPLESALIFTLHVYQYSLSRKGGVAGGRSRLHIIDLGGCANRNGGLPLSGIGNILLAILSGQRHPPHKDHPLTPLLKDCLAPITCHVAIVAHVRPEQSYQDALSTIQIASRIHRA",
    "response#3": "KVKVMLRVADRDRNSGGTEPDFMALDKKKRQVTLTDPRTACPPPQAAQERAPMVAAPKMFAFDNLFTGEDKQSDVCASALSEVIPAVLEGSDGCLLAMGYPATGQAQTVLGELGGGSGSGSASGSGVACSLGAAPCAIAWLYKGIQERRQKSGARFSVRVSAVGVSATKPDALSQDLLISHAAESDDSPGIYLRDDFLGGPTELRAPTAERAALFLDSALAGRLKSSGSTASGSSGCAAPLESALIFTLHVYQYSLSRKGGVAGGRSRLHIIDLGGCANRNGGLPLSGIGNILLAILSGQRHPPHKDHPLTPLLKDCLAPITCHVAIVAHVRPEQSYQDALSTIQIASRIHRR"
  },
  {
    "reference": "MSGAGVGGGIVIVDKPAGMTSHDVVGRCRRIFGTRKVGHAGTLDPMATGVLVVGIDRATKLLGLLTATDKSYEATIRLGQTTTTEDAEGDVVETTPATGITDEQIGHAVAALRGEIDQVPSAVSAIKVGGQRAYKLAREGQTVELAARRVRIDRFEVLAIRRVDGFVDVDVAVDCSSGTYIRALARDVGVTLGVGGHLTLLRRTRVGRFGLDEAYPLDALADEPRLSHSLDEACLLSFPRRDLTPAEAESTRHGRALAPAGIDGVYAAAAPDGSVLALLEDGPQRTKSVVVLRPATL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must possess RNA binding to support tRNA pseudouridine synthesis under certain conditions.2. The Nucleophile active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "ASKMLLGTHNFSAFRSANSENAFKSPIKTLQQADINPSCGILTHHWQSRNLQFWDFTFRARSFLYKQVRRMTGALVAVGQGRLTPQQIKEFLENQDPNCFLGNFVAPPHGLFLKDVEYR",
    "response#2": "ASKMLLGTHNFSAFRSANSENAFKSPIKTLQQADINPSCGILTHHWQSRNLQFWDFTFRARSFLYKQVRRMTGALVAVGQGRLTPQQIKEFLENQDPNCFLGNFVAPPHGLFLKDVEYR",
    "response#3": "ASKMLLGTHNFSAFRSANSENAFKSPIKTLQQADINPSCGILTHHWQSRNLQFWDFTFRARSFLYKQVRRMTGALVAVGQGRLTPQQIKEFLENQDPNCFLGNFVAPPHGLFLKDVEYL"
  },
  {
    "reference": "MNFQNLANQMKKMQREMEKKLAEFEEKEYQYEYKKLIKTTLKGNLRIVKLEINKDLIDPEDPDTLQDMVTEAINEALGDLEKKRDELSNSITPPIKFPGF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Binds to DNA and alters its conformation; May be involved in regulation of gene expression, nucleoid organization and DNA protection.2. The designed protein localizes to the bacterial nucleoid, cytoplasm. The designed protein sequence is ",
    "response#1": "MNTEPNT",
    "response#2": "MPN",
    "response#3": "MF"
  },
  {
    "reference": "MEDPPILTPNPAHSAAKAAPTEQSSVIEIWGGSSLAGHIPISGAKNSALVVMAGTLLCSQQCRVRNIPALMDIDRMSQVLLALGLKLEYKDSVLEVDASRVSQARAPYEIVSKLRASFFIIGPLLARLGVARVPLPGGCAIGDRPVELHIRGLQALGAEVHIDHGTVHAYVPGHRGKLQGGHIYLDYPSVGATETLMMAATLAEGETLIENAAQEPEVVDLANFCRAMGAKIRGAGTNTIMISGVPSLHATDYSIIPDRIEAGTYLIAGAITRAHLSVGPVLPEHLTPLISKLKETGSEIISEGASQLRIRPGRRVIATDIQTLPYPGFPTDLQAQFMALMTLAEGNSMITETVFENRLQHVAEFNRMGADIRVKGDCAIVRGVPRLSGAPVTGSDLRASAALAIAGLAADGKTTLHGLQHLDRGYEKFEQKLRQVGAQIERMPLPLEADADTASPASSSVQSPAAK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The Proton donor active site of the designed protein must be optimized for substrate binding and specificity.2. The designed protein should contain one or more Enolpyruvate transferase domains that are essential for its function.3. The protein design must be focused on enhancing the efficiency of cell cycle.4. The protein design should consider the endogenous proteins and enzymes present in the peptidoglycan biosynthesis pathway to avoid interference with native metabolic processes.5. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "TGGTPLEGTVRAAGAKNAITKLLVASLLSDKKCIFTNVPNIVEVAVTIDLCKEIGSEISWDREAGILEIQTKELKTSYVPQRFSGANRIPILMIGALLGRTDEAIIVPTAGGCKIGSRPVDFHIVALEKLGAQIEYRNMRTEGAYFAHAHGGLKGTLIELPFPSVGATENTIMAAARARGTTIIKNAAIEPEIIDLILFLQKLGVVISVDVDRTIQIQQAKVFYEVEHRVITDRIEVASFAMAAIATKGRVFIEGAEHPHMITFLNKLREVGGGFEVKEGGIEFFYAGPLRGGIHIETDVHPGFLTDWQQPFVTLLTQAEGTSVIHETIYENRFGYAKTLMEMGADIHLYTECLGGKNCRFASEDFHHSLIVKGPTQLSGRTIAIPDLRAGFAYVMAALIAPDESVLTGLHFLDRGYENLTEKLL",
    "response#2": "TGGTPLEGTVRAAGAKNAITKLLVASLLSDKKCIFTNVPNIVEVAVTIDLCKEIGSEISWDREAGILEIQTKELKTSYVPQRFSGANRIPILMIGALLGRTDEAIIVPTAGGCKIGSRPVDFHIVALEKLGAQIEYRNMRTEGAYFAHAHGGLKGTLIELPFPSVGATENTIMAAARARGTTIIKNAAIEPEIIDLILFLQKLGVVISVDVDRTIQIQQAKVFYEVEHRVITDRIEVASFAMAAIATKGRVFIEGAEHPHMITFLNKLREVGGGFEVKEGGIEFFYAGPLRGGIHIETDVHPGFLTDWQQPFVTLLTQAEGTSVIHETIYENRFGYAKTLMEMGADIHLYTECLGGKNCRFASEDFHHSLIVKGPTQLSGRTIAIPDLRAGFAYVMAALIAPDESVLTGLHFLDRGYENLTEKL",
    "response#3": "TGGTPLEGTVRAAGAKNAITKLLVASLLSDKKCIFTNVPNIVEVAVTIDLCKEIGSEISWDREAGILEIQTKELKTSYVPQRFSGANRIPILMIGALLGRTDEAIIVPTAGGCKIGSRPVDFHIVALEKLGAQIEYRNMRTEGAYFAHAHGGLKGTLIELPFPSVGATENTIMAAARARGTTIIKNAAIEPEIIDLILFLQKLGVVISVDVDRTIQIQQAKVFYEVEHRVITDRIEVASFAMAAIATKGRVFIEGAEHPHMITFLNKLREVGGGFEVKEGGIEFFYAGPLRGGIHIETDVHPGFLTDWQQPFVTLLTQAEGTSVIHETIYENRFGYAKTLMEMGADIHLYTECLGGKNCRFASEDFHHSLIVKGPTQLSGRTIAIPDLRAGFAYVMAALIAPDESVLTGLHFLDRGYENLTEKLA"
  },
  {
    "reference": "MPLKIRLSRGGAKKRPFYRVVVADSRMPRDGRFIERLGVFDPLKAKDSAERVVLDAEKAKEWIAKGAQPTDRVARLLDGLGVLTREAQSNPKKALPKKKAQERAAASAAAAEKAAAAAAPEA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein can be targeted to ribosome, ribonucleoprotein complex, cytoplasm and perform structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "MFH",
    "response#2": "MITN",
    "response#3": "ETVNLDRLWTLVPNETREKYLGKNTEVAPVINVLQSGYGKVLGKGRLPDTPVIIQTRYVSRRAEEKIKQAGGVVV"
  },
  {
    "reference": "MKWLAIILVVALLALQYRLWMGEGSIASVVSLNREIAKQKEENARLRERNRLLAAEVDALKQGKDAIEERARNDMGMIKEGETFFMIVDETDKDTKKNKK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must contain a signal peptide for proper functionality.2. The protein must exhibit the following characteristics: Essential cell division protein.3. The designed protein should have a specific function within the cell division site.4. The Periplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells. The designed protein sequence is ",
    "response#1": "RWGDKAVLNKQGEVLIPDDIDEFKNLPLITGPEGQEKKLLEIMKGVYIVLKDKSMQLAEFHVNDRRAWRIKLASGLEMQLGRKAPLENMQRFLKTMDLLGEEQVAMMASVDTA",
    "response#2": "RWGDKAVLNKQGEVLIPDDIDEFKNLPLITGPEGQEKKLLEIMKGVYIVLKDKSMQLAEFHVNDRRAWRIKLASGLEMQLGRKAPLENMQRFLKTMDLLGEEQVAMMASVDTV",
    "response#3": "ARWGTGRLLSDGGDVFSEAEPTIHPELPLLHGPVSQSKMMMRRYNEINQLFHPANLRLKELYLTERMTWFMQFDSGLRIIVDQDQTMNKLQRLSHLAQSDLKPVWSKISAIDLL"
  },
  {
    "reference": "MRFGWLEVAALTAASVANAQVFDNSHGNNQELAFSPPFYPSPWADGQGEWADAHRRAVEIVSQMTLAEKVNLTTGTGWEMDRCVGQTGSVPRLGINWGLCGQDSPLGIRFSDLNSAFPAGTNVAATWDKTLAYLRGKAMGEEFNDKGVDILLGPAAGPLGKYPDGGRIWEGFSPDPALTGVLFAETIKGIQDAGVIATAKHYILNEQEHFRQVGEAQGYGYNITETISSNVDDKTMHELYLWPFADAVRAGVGAVMCSYNQINNSYGCQNSQTLNKLLKAELGFQGFVMSDWSAHHSGVGAALAGLDMSMPGDISFDDGLSFWGTNLTVSVLNGTVPAWRVDDMAVRIMTAYYKVGRDRLRIPPNFSSWTRDEYGWEHSAVSEGAWTKVNDFVNVQRSHSQIIREIGAASTVLLKNTGALPLTGKEVKVGVLGEDAGSNPWGANGCPDRGCDNGTLAMAWGSGTANFPYLVTPEQAIQREVISNGGNVFAVTDNGALSQMADVASQSSVSLVFVNADSGEGFISVDGNEGDRKNLTLWKNGEAVIDTVVSHCNNTIVVIHSVGPVLIDRWYDNPNVTAIIWAGLPGQESGNSLVDVLYGRVNPSAKTPFTWGKTRESYGAPLLTEPNNGNGAPQDDFNEGVFIDYRHFDKRNETPIYEFGHGLSYTTFGYSHLRVQALNSSSSAYVPTSGETKPAPTYGEIGSAADYLYPEGLKRITKFIYPWLNSTDLEDSSDDPNYGWQDSEYIPEGARDGSPQPLLKAGGAPGGN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Ensure the presence of a signal peptide within the protein.2. The protein must exhibit the following characteristics: Beta-glucosidases are one of a number of cellulolytic enzymes involved in the degradation of cellulosic biomass.3. The design objective is to achieve a protein with helix-helix-helix-strand-helix-strand-helix-helix-strand-helix-helix-strand-helix-strand-helix characteristics.4. The designed protein must have a active site that is structurally stable and resistant to denaturation.5. We require a protein that can specifically bind to the metabolites produced in the Glycan metabolism, cellulose degradation pathway.6. A protein that contributes to glucan catabolic process by functioning in extracellular region with beta-glucosidase activity. The designed protein sequence is ",
    "response#1": "LEAAMKAAGKQYFGTALTVRNDQGEIDIINNKNEIGSITPENAMKWEAIQPNRGQFNWGPADQHAAAATSRGYELRCHTLVWHSQLPSWVANGNWNNQTLQAVMRDHINAVMGRYRGKCTHWDVVNEALNEDGTYRDSVFLRVIGEAYIPIAFRMALAADPTTKLYYNDYNLEYGNAKTEGAKRIARLVKSYGLRIDGIGLQAHMTSESTPTQNTPTPSRAKLASVLQGLADLGVDVAYTELDIRMNTPATQQKLQTNADAYARIVGSCMDVKRCVGITVWGISDKYSWVPGTFPGEGSALLWNDNFQKKPSYTSTGRSTRYWDCCKPSCGWAKKAPVNQPVFSCNANFQRITDFDAKSGCEPGGVAYSCADQTPWAVNDDFALGFAATSIAGSNEAGWCCACYELTFTSGPVAGKKMVVQSTSTGGDLGSNHFDLNIPGGGVGIFDGCTPQFGGLPGQRYGGISSRNECDRFPDALKPGCYWRFDWFKNADNPSFSFRQVQCPAELVARTGCR",
    "response#2": "MKWEAIQPNRGQFNWGPADQHAAAATSRGYELRCHTLVWHSQLPSWVANGNWNNQTLQAVMRDHINAVMGRYRGKCTHWDVVNEALNEDGTYRDSVFLRVIGEAYIPIAFRMALAADPTTKLYYNDYNLEYGNAKTEGAKRIARLVKSYGLRIDGIGLQAHMTSESTPTQNTPTPSRAKLASVLQGLADLGVDVAYTELDIRMNTPATQQKLQTNADAYARIVGSCMDVKRCVGITVWGISDKYSWVPGTFPGEGSALLWNDNFQKKPSYTSTLNTFLDRAPEGNYVDATFYTSDEALEAATAPLYNRAWFDYNQRSIVPIGSGRANDMYSPWNYPQFVTFQVTALDENLSGAWSGFYSVVTMANSVINAVETQTQGSVSEAAKTKAIAEARLMRACAYFYMLRIWGPVILIEDNQKLVDNPVRPLNREEDVFQFIINDLNYAVDNLSEQSDKGRATSWAAKGILAKVYAKK",
    "response#3": "GRSTRYWDCCKPSCGWAKKAPVNQPVFSCNANFQRITDFDAKSGCEPGGVAYSCADQTPWAVNDDFALGFAATSIAGSNEAGWCCACYELTFTSGPVAGKKMVVQSTSTGGDLGSNHFDLNIPGGGVGIFDGCTPQFGGLPGQRYGGISSRNECDRFPDALKPGCYWRFDWFKNADNPSFSFRQVQCPAELVARTGCSVG"
  },
  {
    "reference": "MNLSIVILAAGAGTRMKSKTPKVLHTLCGREMLYYVIKEAKKLSDDVSVVLFHESEMVKKSIEKYFSDIRYVIQDHVNYPGTGGALMGIEPKYQKILVLNGDMPLVEAEELKQFLRAGSDFVMSVLDLEDGSGYGRVVIHGGSVERIVEEKDATGEEKSLGTVNAGVYLFDRALLACYLPRLKNDNAQKEFYLTDVVEMARKDRLIVAPLFVKEENFKGVNSKADLAEAEAIMTGRIRRRWMREGVRMRLPETIYIDEGVKFVGECFIENGVSITGDSYIEESHIKAHSVIEESRVIRSDVGPMAHLRPQCEVTETHIGNFVEVKKSKLTGVKAGHLSYLGDCEVDEGSNIGAGVITCNYDGKKKHLTKIGKNVFVGSDSQLVAPVSIEDDSIIGAGSTITKNVKSGELALSRAKQENIAGFFARYFSSSK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein with magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity functions is required to enable cell wall organization, cell morphogenesis, peptidoglycan biosynthetic process, lipid A biosynthetic process, regulation of cell shape.2. The acetyl-CoA, Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.3. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate + UTP =   diphosphate + UDP-N-acetyl-alpha-D-glucosamine are properly positioned for optimal catalysis.4. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The designed protein sequence is ",
    "response#1": "LILAAGEGTRMRSSTPKVLHPIAGRPLVEHAVRAAAGLNPEHLVVVVGHGRELVGEHLAQLGTALGRPVGTAVQDQQKGTGHAVSCALATLPADVTGTVIVSYGDVPLLDTTTLAALLDEHTEAKNAVTVLTTVVDDPTGYGRILRDAAGQVVRIVEQKDATPEERRIREVNTGVVAVPAGRLRRWLAALDSDNAQGEYYLTDAVALAAADGVPVVATHPDHVVETEGANDRVQLAELGAELNRRVVRGWMAAGVTVMDPATTWIEVDVQLAPDVTILPGTQLLGATVVEEDAVIGPDCTLQDVEVGAGARVVRTHGQLAVIGAGATVGPFSYLRPGTRLGAGGKIGAFVETKNAQIGDGAKVPHLSYVGDAEIGEGTNIGAGTIVANYDGVHKHRTVIGKHARTASNNTFVAPVTVGDGAATGAGSVIREDVPAGALAVTAGPEQRNIEGWVQRKRPGSTAAQAADRAAQSEGGD",
    "response#2": "IILAGGQGKRMKSDMPKPMFKVLGEPMLQWVISAGEDAGISNICIVKGFKGEVIDEYINGKYETVLQSERLGTGHAVMQAVPFLEKNPDGNTLVLCGDAPFIDSETIKESLELHVQQKNSVTVITAELDNPFGYGRILRTENGISGIVEQKDADENQQNIREVNSGAYWFNTADLMDFLKKLTNNNSQNEYYLTDTVEIAVKSGKRAGAYLTPDPLEVMGVNTRVQLAEAADIIRRRINRHLMLAGVHLIDPARFDLRGHLTIGKDVRIDINVIIEGECELGDNVEIGAGCIIKNTKISANTKVQPYSVFDNAFVGESVQIGPFARLRPGAKLANEVHIGNFVEVKNSSIGFASKANHFAYLGDAQIGSGSNIGAGTITCNYDGANKHKTVIGDDVFIGTNNSLVAPVKIGNGATTGAGSTITRDVADNALAVERSQQDNIKDWGVHLIDPARFDLRGHLTIGKDVRIDINVIIEGECELGDNVEIGAGCIIKNTKISANTKVQPYSVFDNAFVGESVQIGPFARLRPGAKLANEVHIGNFVEVKNSSIGFASKANHFAYLGDAQIGSGSNIGAGTITCNYDGANKHKTVIGDDVFIGTNNSLVAPVKIGNGATTGAGSTITRDVADNALAVERSQSYPQMKKGVK",
    "response#3": "VVILAAGQGTRMKSRRSKVLHEIAGRPMVGFPVALARELSASEPVVVVGRDAEDVQAALAGQAKFVLQAEQNGTGHAVLCAQDALADARDAILILYGDTPLLRLESLRRMAEMLAADDADLVMLTSPEPTGYGRIIRDDEGNVVAIVEERDATPEQRAITEVNSGVYAFDIAALRSALGRLSSENAQGELYLTDVVAIARGDGHRVGALMAADHREIVGVNNRVQLAEARRLLNERLLERAMLAGVGIVDPETTWIDSTVTIGADTVIRPGVQLHGTTAIGADCEIGPDSTLTDITVGDGACVVRTHGSGASIGEGASVGPFTYLRPGTVLGAAGKIGGFCETKNAQIGDGAKVPHLSYVGDAQIGEGTNIGAATIFANYDGVNKHRTVVGKHVRVGSDNVLIAPVTIGDGAATGAGTTVLEDVAPGDLAINTVSPATRKPGWVARKRAGTKSAEAAKQAAESSGPTDSDA"
  },
  {
    "reference": "MRLLLFNQNAFLLACMFVSSVYVNAVLDAYAIENPYISITLTSLLAPLSMLAFLKTPRNSAFALGFFVGALLFYWCALSFRYSDFTYLLPLIIVLIALVYGVLFYLLLYFENPYFRLLSFLGSSFIHPFGFDWLVPDSFFSYSVFRVDKLSLGLVFLACIFLSTKPLKKYRIIGVLLLLGALDFNGFKTSDLKKVGNIELVSTKTPQDLKFDSSYLNDIENNILKEIKLAQSKQKTLIVFPETAYPIALENSPFKAKLEDLSDNIAILIGTLRTQGYNLYNSSFLFSKESVQIADKVILAPFGETMPLPEFLQKPLEKLFFGESTYLYRNAPHFSDFTLDDFTFRPLICYEGTSKPAYSNSPSKIFIVMSNNAWFSPSIEPTLQRTLLKYYARRYDKIILHSANFSTSYILSPSLLGDILFRKRS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Catalyzes the phospholipid dependent N-acylation of the N- terminal cysteine of apolipoprotein, the last step in lipoprotein maturation. The designed protein sequence is ",
    "response#1": "ATMSKVFSLAIPTFENSPSLIVIPESAIPFHGTIDSEENRKEKIYSSTMEGIILYLSKHTGADVLFNELNLDENKLRNQVSLFKNLDGKTERYNKRRLLPFGEYLPMEKNFPFLRSIFQETSRYVPGEFPKLLIGNKIQNQRSFLPPEISKLNEPKTYRYEFSSIVEHTNKIRNLEYSYSILPLLCYEAMFTELVLDYFQNEQKPEVLINITNDSWFDSELEAYQHSGAVRLRAIETGLPLIRSAVSGISEVWDARGIPMIVPIGFHETGTRAFSIRLDAIESTCA",
    "response#2": "WQTNMPTREKTNFKNQFINDKLISAQKIALSNDAKLLITPEGTLNNNFNLNFKSKIRMLAGGFRNSKNGLRSSLLGYQIGDKTYSSFIDKHRLVPLGEKIPGFLNIFSRGLSAVGGIQPGSDSRFFKWKFTQPLAIAICYEITDGFKIRNAVKSGAELIISAANLDPYPRKLHYQFLSLARVRSIENKKDNIIISNTGPSGLISEEGKIIKLFDPNTEQNDVVNPNFSVN",
    "response#3": "WQTNMPTREKTNFKNQFINDKLISAQKIALSNDAKLLITPEGTLNNNFNLNFKSKIRMLAGGFRNSKNGLRSSLLGYQIGDKTYSSFIDKHRLVPLGEKIPGFLNIFSRGLSAVGGIQPGSDSRFFKWKFTQPLAIAICYEITDGFKIRNAVKSGAELIISAANLDPYPRKLHYQFLSLARVRSIENKKDNIIISNTGPSGLISEEGKIIKLFDPNTEQNDVVNPNFSR"
  },
  {
    "reference": "MKVAVVGATGLVGSKMLQVLEERNFPITELIPVASEKSVGKEVTFKGKPYKVVNADTAISMKPAIALFSAGGSTSLEMAPKFAAAGITVIDNSSAWRMDPSKKLIVPEVNGDTLTAADKIIANPNCSTIQMVMVLSPLHKQYKVNRVVVSTYQSVTGTGVKAVTQLMNERQGIEGEKAYAYPIDLNVIPQIDVFTDNGYTKEEMKMVKETVKIMGDDNIRVTATTVRIPVIGGHSEAVNIEFENEYEVSAVREILSNTPGVVVVDDPSNLQYPMPKDAHDKDEVFVGRIRRDETQPKTINMWIVSDNLRKGAATNAVQIAEYLHAQKWV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that can modulate the overall flux of metabolites through the Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5, L-threonine biosynthesis pathway, enhancing or suppressing its activity as needed.2. A key design requirement for the protein is the optimization of the Proton acceptor, Acyl-thioester intermediate to achieve high catalytic efficiency for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.3. The designed protein should have protein dimerization activity, NADP binding to facilitate lysine biosynthetic process via diaminopimelate, 'de novo' L-methionine biosynthetic process, isoleucine biosynthetic process, diaminopimelate biosynthetic process.4. The NADP(+), phosphate, substrate binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "LGGLFRENLVEWATSMTYQAASGAGAQNMRELLQQMGEAHRVAGDLLRDPASAILDIDREVAGTLRDERFPTAHFGVPLAGSLIPWIDKDLGNGQSKEEWKGGAETNKILGREANPTPIDGLCVRIGAMRCHSQALTIKLTRDVPLDEIDAIIASANSWVKVVPNEREASMHELTPTAVTGTLAVPVGRLRKMNMGSEYLSAFTVGDQLLTVPDSYRVLPLKPLHDYAKIKRVVVATYQSVSGAGKRAMEELASQSRDILNMKEIRIEKFPHQIAFNCLPHIDVFLDNGYTKEEMKMVNETKKIMEDDSIAVTATTVRVPVFLSHSEAVNVEFENDITVEKARELLGNFPGIVVVDDVKNAKYPLAIYAAGKNEVFVGRIRKDVTVKSGLDMWVVADNLRVPISSPRINHPILAIFGATGLVGETMRSILEERNLPIDNIYFFASERSAGKKIKFLDKEYEIIELSEENLKKEKIDYALGALDSSLAKKFLPIAAKLGIKIIDNSSSYRMDEDKPLVVPEINPQGLFVNNLIDWVFVSTYQAASGAGSRYVIELLKQMGSLYNVVSKDLLDKSYSVLDIEKKVTQESRSKNFPLENFSVPLATSLIPWIDVEMKNGQSREEWKGQAETNKILNLKKKVLIDGTCVRISSIRCHSQSFLIKLNKDISLENIKKIIVNHNQWVDVIPNNMQKTLCNLTPSAVTDTLNIPIGRLRKLNIDDRYLSAFTVGDQLLIK",
    "response#2": "MNVAVVGATGAVGNEMVSLLEERNFPVKNLRLLASERSLGKRIKFKGEDIPVEVLSKDSFKGIDYALFSAGAARSKEFCPIAAESGAVAVDNSSAFRMEPDVPLVVPEVNPHANANRIVSATCVRVPVFYGHLAPLHNKYKIKRIVVSTYQSVSGTGVKAVEQMENEILGKKGEMAYPYPIHKNAIPHCDVFEDNGYTKEEMKLVNETQKILSDRTIAVTATAVRIPTAGGHSEAINVEFENEFDLTEVRQLLNNTDGITIQDNLETNSYPMPMYAHGKDDVFVGRIRRDGSQPNTLNMWVVSDNLRKGAALNAVQLYPIYKKYGIKRIVYTTYQAVSGSGKNGLEDLERGMKGQENIFYPYPIIGNVLPHIDDFLENGYSKEEMKMINETKKIFHDNSIKITATTARVPVKTSHMVAINVETKEDFDIKDIFELYKNTDGIVLYDDLKNLKYPMPIVAAGKDPVYVGRIRRDISVEHGLDLICVADNMRKYYNHSDEIVFGLFVNNLIDWVFVSTYQAASGAGSRYVIELLKQMGSLYNVVSKDLLDKSYSVLDIEKKVTQESRSKNFPLENFSVPLATSLIPWIDVEMKNGQSREEWKGQAETNKILNLKKKVLIDGTCVRISSIRCHSQSFLIKLNKDISLENIKKIIVNHNQWVDVIPNNMQKTLCNLTPSAVTDTLNIPIGRLRKLNIDDRYLSAFTVGDQLLNIS",
    "response#3": "MSKVGFVGWRGMVGSVLMQRMREERDFDLVEPVFFTTSNPGGKGPDVGRDVPPLKDARSLAELKAMDIIVSCQGGDYTTEIFPKLRSDGWNGHWIDAASTLRMEKDAVIILDPVNRNVIQDALKRGVKDYIGGNCTVSLMLMALNPLHDKYKINRVVVSTYQSVTGTGVKAVDQLMNERQGIEGEKAYAHRIDLNVIPQIDVFLDNGYTKEEMKMVKETVKIMGDSNIRVTATTVRIPVIGGHSESVNIAFENEYDLAEVRSILENTPGVTVVDDPSKALYPMPKDAHEKDDVFVGRIRRDETQEKTLNMWIVADNLRWGAAEPLRRMLRILLGR"
  },
  {
    "reference": "MRVKGTRKNYLWRWGTMLLGMLMICSATANLWVTVYYGVPVWKEANTTLFCASDAKAVNTEKHNVWATHACVPTDPNPQEVVMGNVTENFNAWKNNMVEQMQEDMISLWDQSLKPCVKLTPLCVTLECSNLNKTANLSDNGKEELKNCSFNVTTSIRDKVRKEYALFYNLDIVQIDDKNNNTNNTKYRLTHCNTSVITQACPKASFEPIPIHYCAPAGFAILKCNNESFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEKEIVIRSENISDNAKTIIVQLKKPVQINCTRPNNNTRKSIPIGPGKAFFATGEIIGDIRQAHCILNRTQWNNTLGEIAKKLGEIFGQNKTIVFNKSSGGDPEIVMHSFNCGGEFFYCNSTPLFNSTWNANGTWNGTGNLTTGNITLTCRIKQIINMWQEVGKAMYAPPIRGLISCSSNITGLLLTRDGGEGNDTNNITEVFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGALFLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNDSWSNKSMKDIWDNMTWMQWDREITNYTKVIYSLIEESQNQQEKNEQELLALDKWDSLMNWFSITNWLWYIKIFIMIVGGLVGLRIVFTVLSVVNRVRQGYSPLSFQTHLPIPREPDRPEGIEEEGGERDRDRSTRLVHGFLPLIWDDLRSLILFSYRRLR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Include a signal peptide as an essential component of the protein.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The designed protein should be expressed specifically in the plasma membrane The designed protein sequence is ",
    "response#1": "GNGAVLWLLSSNVYRNPFAIYLLDVACADLIFLGCHMVAIVPDLLQGRLDFPGFVQTSLATLRFFCYIVGLSLLAAVSVEQCLAALFPAWYSCRRPRHLTTCVCALTWALCLLLHLLLSGACTQFFGEPSRHLCRTLWLVAAVLLALLCCTMCGASLMLLLRVERGPQRPPPRGFPGLILLTVLLFLFCGLPFGIYWLSRNLLWYIPHYFYHFSFLMAAVHCAAKPVVY",
    "response#2": "GNGAVLWLLSSNVYRNPFAIYLLDVACADLIFLGCHMVAIVPDLLQGRLDFPGFVQTSLATLRFFCYIVGLSLLAAVSVEQCLAALFPAWYSCRRPRHLTTCVCALTWALCLLLHLLLSGACTQFFGEPSRHLCRTLWLVAAVLLALLCCTMCGASLMLLLRVERGPQRPPPRGFPGLILLTVLLFLFCGLPFGIYWLSRNLLWYIPHYFYHFSFLMAAVHCAAKPVVY",
    "response#3": "GNGAVLWLLSSNVYRNPFAIYLLDVACADLIFLGCHMVAIVPDLLQGRLDFPGFVQTSLATLRFFCYIVGLSLLAAVSVEQCLAALFPAWYSCRRPRHLTTCVCALTWALCLLLHLLLSGACTQFFGEPSRHLCRTLWLVAAVLLALLCCTMCGASLMLLLRVERGPQRPPPRGFPGLILLTVLLFLFCGLPFGIYWLSRNLLWYIPHYFYHFSFLMAAVHCAAKPVVR"
  },
  {
    "reference": "MASYAVLGATGNCGSALIQLLLQRPDASIHAFCRNKPKLIGLLPEIEKSERVTIYQGSIKDTDLLTACLRGRRAVFMVLSSNINEPGFRVGRDANKAVIEVLRTLHREDSGSTGPPPKIILLSSATLDDTLSRKTPYLLRQILHMSASHIYNDLAETEKLLRAEQEWLTTVFVKPGALSVDEQRGHALSFVESDNPVSYLDLAAAMIEAADDTTDKYDMKNIGVVNTNGAARFPTGTPLCIATGLLRHFVPSLHPYLP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Endocrocin could be then modified to clavorubin, possibly by CPUR_05423 and CPUR_05431; Ergochromes formation requires further dimerization steps of different xanthone units, probably catalyzed by the cytochrome P450 monooxygenase CPUR_05419; The ergochrome gene cluster produces several ergot pigments including the yellow ergochrome secalonic acid and its derivatives, as well as the red anthraquinones endocrocin and clavorubin; Emodin is further modified to yield monodictyphenone via several steps involving CPUR_05427, CPUR_05428, CPUR_05429 and CPUR_05430 ; CPUR_05425, CPUR_05426 and CPUR_05431 are unique to Claviceps, thus it is likely that they are involved in further modification of xanthone units or in their dimerization. The designed protein sequence is ",
    "response#1": "LNVLVVGAGPSGLMTALECWRKGHNVVKILERSNSPVFTGDVIVIGPSALRAFRHWPDMCAELEKSKMDSIMYYRKHNGELILGPTSLGHNDPEYTAAWKGIPFAAPHQIRKDFYRMLLRQVARVGIRVEYGQRVEKYFDDEAAMLGGVITDQGTIMFADLVVAADANRTRSDLLIAGAHTPSRSSGMSVYRTAFPTERALQDEAFRTRWGDAIEKGISHHEFWMGPGMHLGLFISPEFVAFGLTPRDSFLHEGGNEPIESWEPDVDPEEVTKVLNRVPDWNPVIKSLVKNTQRGSIVHWPLLWRNLRREWTSKSGRVVQAGDAAHSSIPASISGGTLALEDAVTLASCLHLSF",
    "response#2": "ETKKSFTPDIDVVETPESYSVQASLPGAKKEDVKVSWDPSTYELRIEGVVSRSVGNDEEGEKKTEQSGRFSLRERQTGKFCRTVYLGSQVDGDKIEEGGLSAGMDDGVLRIAVPRQGSGKGVKEITIK",
    "response#3": "YEDATACQAALFEWAESYDLKDWNRLSKCIAPTLQIDYRAVFGQFWEAMPADEFVGMISSPGFLGNPLIKTQHFVGMTRWTQTSEDSVTGRHQMRVAHQKYADAQMKEVVLKGHGHGGATTWFRKVDGDWKFAGIEPEIRWAEFDLDKIFE"
  },
  {
    "reference": "MALATIEQALEELRAGRMIIVVDDEGRENEGDLVMAAEKVSPEAVNFMAAYGRGLICVALTGERLDALRIPMMVSPDSNASRFQTAFTVSVDARHGITTGISAYDRAVTIRALVDPATRPEDLVMPGHVFPLRAREGGVLARAGHTEAAVDLARLAGLRPAGVICEILAEDGSMARLPRLEAFAAAHRLKIISVADLIAYRRRHEKLVRCVAQAHLPTRHGTFAILAYEEIVTGTCHLALVMGDVADGRPVLVRVQSECLTGEVFGSLRCDCGSQLDLAMQRIGAEGRGIVLYLRQEGRGIGLCNKLRAYALQELGMDTVEANEQLGFPPDLRDYSVGAQILADLGVRELRLLTNNPHKVAGLTEYGLRVVERVPLVVQPTPENRFYLQAKREKMGHLLETI",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a binding site that is specific to the target Zn(2+), GTP, D-ribulose 5-phosphate ligand.2. The designed protein should contain one or more GTP cyclohydrolase II domains that are essential for its function.3. The protein with the reaction GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate, the Proton acceptor; for GTP cyclohydrolase activity must be optimized to ensure efficient catalysis.4. A protein should be able to manipulate the activity of enzymes in the Cofactor biosynthesis, riboflavin biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway, either by activating or inhibiting their function.5. Zn(2+) is required for the proper folding and stability of the protein.6. For general function, the protein need meet that Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.7. A protein with GTP binding, magnesium ion binding, GTP cyclohydrolase II activity, zinc ion binding, manganese ion binding functions is required to enable riboflavin biosynthetic process. The designed protein sequence is ",
    "response#1": "MSNAFLPTKFGDFKIILYHSETDSKDHVALVKGEISPDKPTLVRVHSECLTGDVFGSLRCDCNDQLQSALKIINEAGSGVVLYMRQEGRGIGLSGKIRAYRLQDEGLDTVEANEKLGFRADLRDYGIGAQILLDLGVRKIRLLTNNPKKIVGLEGYGLEIVERIPEPLPERVTQAHMPTKYGNFKMVGYVNKVNGEHHIALVKGDITDGEPVLMRVHSECLTGDAFGSLRCDCGDQFAAAMKKINEEGRGLLLYMRQEGRGIGLINKIKAYALQDTGMDTVEANLALGFSEDMRDYGIGAEIIRDLGIKKIKLMTNNPKKINGLEGYGLEIVERVPYEEV",
    "response#2": "GVDMPTEWGHFDLIAYRQTNTDELHLALVKGTWDKDEPVLVRVHSSCVTGDIFGSCRCDCGGQLHAAMEMVDKAGKGVVLYMNQEGRGIGLLNKLKAYKLQEMGRDTVDANLELGLPMDSRDYGVGAQILRDLGITKINLISNNPKKRVGLMGYGLEIVDSVPYMDEPKILEKEAEAELPTVYGNFAIKAYTQTTNDKEHLALIKGTWNKDEAVLVRVHSSCITGDIFGSCRCDCGPQLHAALQMIEKEGKGVLVYMNQEGRGIGLVNKLKAYKLQEQGRDTVEANLELGFKMDERDYGVGAQILRDLGISKIKLLTNNPKKRTGLIGYGLEIVENIP",
    "response#3": "MTNAFLPTKFGDFKIILYHSETDSKDHVALVKGEISPDKPTLVRVHSECLTGDVFGSLRCDCNDQLQSALKIINEAGSGVVLYMRQEGRGIGLSGKIRAYRLQDEGLDTVEANEKLGFRADLRDYGIGAQILLDLGVRKIRLLTNNPKKIVGLEGYGLEIVERIPLPEVPPGTPHASARMPTKYGDFQIYGYVNDITGEHHVALVKGDLGDGENVLCRVHSECLTGDTFGSRRCDCGEQLQRAMQQVEKEGRGIILYMRQEGRGIGLINKLKAYELQEQGLDTVEANIRLGFDADLREYWVGAQILADLGARSLRLLTNNPDKIYGLEGFGLTITERVERVTQAHMPTKYGNFKMVGYVNKVNGEHHIALVKGDITDGEPVLMRVHSECLTGDAFGSLRCDCGDQFAAAMKKINEEGRGLLLYMRQEGRGIGLINKIKAYALQDTGMDTVEANLALGFSEDMRDYGIGAEIIRDLGIKKIKLMTNNPKKINGLEGYGLEIVERVPLPPVPPGTPHGVDMPTEWGHFDLIAYRQTNTDELHLALVKGTWDKDEPVLVRVHSSCVTGDIFGSCRCDCGGQLHAAMEMVDKAGKGVVLYMNQEGRGIGLLNKLKAYKLQEMGRDTVDANLELGLPMDSRDYGVGAQILRDLGITKINLISNNPKKRVGLMGYGLEIVDSVPLP"
  },
  {
    "reference": "MGRKLRKGETLIVASHNPGKIAEIADLVRPFGLAVVSGPALGLPEPEETGDSFRANAALKALAAARGGRMPALADDSGLVVPALGGAPGIHSARWAGPDRDFKAAMTQVAHALDAKGVPAAERRACFVCVLALAWPDGHVETYQGRIDGRLVWPMRGDKGFGYDAIFKPDGQDLTFGEMDSEAKHAISHRARAFAQLVAACLE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The substrate, Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.2. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.3. The designed protein should have XTP diphosphatase activity, metal ion binding, nucleotide binding to facilitate nucleotide metabolic process.4. The Proton acceptor of the protein must be engineered to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+).5. The protein requires Mg(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "MTKNIVLASNNKGKIREFQKLFNDSNFTIIPQSEFNVPEAEETGKTFVENAIIKARNASKHTGLPAIADDSGLEVDYLNGAPGIYSARFAGENATDKDNLDKLLSELSGVPTENRRARFVCVIALIKHSDDPTPIICHGIWEGRILNEPRGKNGFGYDPLFFVPTKKCTAAELSPEIKNEISHRGQAMRQLLNHLQKL",
    "response#2": "MTVILASSNTGKLREFNAMLAPLGIDVRPQSEFGVTEAEEPHPSFVENALAKARHASKLTGLPALADDSGLAVDALNGMPGVLSARWSGRHGDDAANHALLLAQLGDVPDERRGAAFVAALALVRAGDEHLLVEGSWEGRILREPRGSGGFGYDPLFVPEGQPRSAAQLSPEEKDARSHRGRALRAMMPVLLQQLR",
    "response#3": "MTTSETTHFGFREVPIEEKAGRVREVFDSVASRXDXFMGRXGXRFWKRXTVXXSGVRTGXRVLDIAGGTGDLALXFSRIVGDSGQVILADINASMLXVGRDRLVXXGXSXVSXAQCDAEVLPFPDXHFDAVTIAFGLRNMTHKDQALSEMLRVLRPGGXLFCLEFSXVXTPXLAXVYXAYSXLLPLMGXIVANDXASYRYLAESIRMHPDQETLKGMMXAAGFERCDYHNLTGGIAALHAGXKW"
  },
  {
    "reference": "MMTDPLSRSHAAALDAADPLRALRDAFVFPQHGGQDQTYFVGNSLGLQPRQARAMVSEVLDQWGALAVEGHFTGPTQWLTYHQLVRDGLARVVGAQPDEVVAMNTLTVNLHLMMASFYRPSAERAAILIEAGAFPSDRHAVESQLRLHGLDPDTHLIEVEPDAADGTLSMDAIAATIAQHGPRLALVLWPGIQYRTGQAFALGEIARLARAQGAAVGFDLAHAVGNIPLSLHDDGVDFAVWCHYKYLNAGPGAVGGCFVHARHAHSNLPRMAGWWGHEQPTRFRMEPQFVPSPGAEGWQLSNPPVLALAPLRASLELFDQAGMPALRAKSEQLTGHLEQLIHTRVPQVLQIVTPADPAQRGCQLSLRVAGGRTQGRALFEYLQSVGVLGDWREPDVIRIAPVPLYNRFCDLHQLVEHVETWAAA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The presence of pyridoxal 5'-phosphate is crucial for the protein to function optimally under specific conditions.2. The pyridoxal 5'-phosphate binding site should be stable and able to withstand changes in environmental conditions.3. The designed protein should have pyridoxal phosphate binding, kynureninase activity, 3-hydroxykynureninase activity to facilitate 'de novo' NAD biosynthetic process from tryptophan, quinolinate biosynthetic process, anthranilate metabolic process.4. The protein should be designed to have high stability and activity towards 3-hydroxy-L-kynurenine + H2O = 3-hydroxyanthranilate + H(+) +   L-alanine. The designed protein sequence is ",
    "response#1": "AELVGARVHEVVVMNTLTINLHLMMTSFFRPQGQRRRLLMERGAFPSDRYAVVSQLQWHGLDPDRDLAELAPRRDRLLHEEDLEDYLERYGDQVAMVLWPGVQYATGQVFDLARIARACRKAGTVLGLDLAHAVGNVPLDLHEIGADFAVWCSYKYLNSGPYTCLDYQERFAEPLSRIVGAKPSEVTVMNTLTVNLHLLMVSFYQPTKTRYKIICEEKAFPSDQYMFQSQVAFHGYKKEETIIEIKRREGEHNIRLEDILAKINEVGDELALVLIGGVNYYTGQVFDMKTITEAGHKVGAKVGFDLAHAAGNIKLDLHDWNVDFAAWCSYKYL",
    "response#2": "AELVGARVHEVVVMNTLTINLHLMMTSFFRPQGQRRRLLMERGAFPSDRYAVVSQLQWHGLDPDRDLAELAPRRDRLLHEEDLEDYLERYGDQVAMVLWPGVQYATGQVFDLARIARACRKAGTVLGLDLAHAVGNVPLDLHEIGADFAVWCSYKYLNSGPGAELVGARVHEVVVMNTLTINLHLMMTSFFRPQGQRRRLLMERGAFPSDRYAVVSQLQWHGLDPDRDLAELAPRRDRLLHEEDLEDYLERYGDQVAMVLWPGVQYATGQVFDLARIARACRKAGTVLGLDLAHAVGNVPLDLHEIGADFAVWCSYKYLNSGPGASFSAEHDGYRAELVGARVHEVVVMNTLTINLHLMMTSFFRPQGQRRRLLMERGAFPSDRYAVVSQLQWHGLDPDRDLAELAPRRDRLLHEEDLEDYLERYGDQVAMVLWPGVQYATGQVFDLARIARACRKAGTVLGLDLAHAVGNVPLDLHEIGADFAVWCSYKYLNSGPGASFSAEHDNYRDYQERFAEPLSRIVGAKPSEVTVMNTLTVNLHLLMVSFYQPTKTRYKIICEEKAFPSDQYMFQSQVAFHGYKKEETIIEIKRREGEHNIRLEDILAKINEVGDELALVLIGGVNYYTGQVFDMKTITEAGHKVGAKVGFDLAHAAGNIKLDLHDWNVDFAAWCSYKYA",
    "response#3": "DYQERFAEPLSRIVGAKPSEVTVMNTLTVNLHLLMVSFYQPTKTRYKIICEEKAFPSDQYMFQSQVAFHGYKKEETIIEIKRREGEHNIRLEDILAKINEVGDELALVLIGGVNYYTGQVFDMKTITEAGHKVGAKVGFDLAHAAGNIKLDLHDWNVDFAAWCSYKYNYLI"
  },
  {
    "reference": "MNTMPGMGVGVGVTVGDCENYVQQQQPQQQQPQLEVTPTHHRLQVRRQSTLPAQPTPAIYMSTSPNRLYSRSPERSPGEQQRYPPFMRQTSFPEPPSTYHRTKLLPSTGAPPPTVAPPPLNYESQTSSLASDEQDVGPMPKPRMTRQATLPNPEQHVKLLPTSPPKRQTSPQFRRSPEFMRQQTLPNPEAFSSGNTLTVHSTPPAKFMPISPRAKQNFLFPSVPNPRQFLSQPHVPAVGGTELGSGGSVASTGVVGGGEQYSSSQSVNIHHSRDPHSKMIKVRSHSNEEYSNTKGHPENRRLLPEIPTAVQRPGGRSPSRLVRQDCLKEERTFGEAKQQFHQFPDVTEELDNRPEYVDYFGDSATSFLESDEVQYERNYNAGFSSEPRIIYNNGSDDGSYQNSSQRPIYSAENVLADSGYGGGAGVGLGASGGSGGVPGYYPDMGTPQGFYGGAALPRTPLMHNRIRRRQSRELQMQQEELAQLSQVSDANGKGSGNEGAASGSATDSHAAERRKPEQMRSVSEDSGAKTTPKPVTRRSFSHPEKDTQPTKKTEISKIPSPRPLADILDKTRGNSKIPQARRRNSRTGIGEAEEGSTPQHIYSFHQQLMHRNSDLAMRLKKTEIPVASVASTGQEEEKPHGESGQGSGAEQPVNGKSLESSANKEGGDPNSSAEATHTSTLGEQELQNAVEAAAVVFKKVVLQRRKEKEKAAEEEEAKVAAEELSRSPVIGQRQADGSGSPIQSRASSETWPTQSDEDIDRLVAMHQN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.2. The protein design must be focused on enhancing the efficiency of actin filament organization. The designed protein sequence is ",
    "response#1": "MCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLWPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNSLMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGR",
    "response#2": "MCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLWPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNSLMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGY",
    "response#3": "MCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLWPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNSLMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGL"
  },
  {
    "reference": "MKVLLLLLLPPLQGVGASGRQEDFRFCGDRNQTKNSSVIYEHSSANISIENMAQALVIRSPFLTNRINSSYQYSLPTTLGRYRFCVYWFKANRTLWLAYGKQSFFLGHEPTDGVTRGLEKTRASIFNVSYVFKGQKNTSLENASEYIFPVPPEMMPVWKQDVEDELAALDGLLARVPSAGSKAQRSLRHKLGALERTLAQVELGGQNQTFGKAALRATVLSISPTQAPRHLAFASPKEESREVQGFAVDLPSSLFVMAKKKEEEVVEHRVLLVDINSQAMFQDENSSHILGDKVVGISLVDTVVANLSEPVVLTFFHNRLPRNVTPRCVFWREDGTASSGNWDSYGCTTVEGDTRTDCRCNHLTYFAVLMVSSPEISYLHRDSLSIITYIGCLISALASISTIFFLYFRSKQRDQITSMHIHMNLLGAIFLLDFTFLISEHLASSSSQAACRAGGLFLHFSLLSCLTWMGIEGYNLYRLVIEVFNAYHDYFLLKLCLVGWGLPFFCVMLIFLANWTNYGPFYIPVYESVGGRSTNATMCWLTSPPAHYMTLCYAGLILLFNVLVLVAVVVVLRKTRQQQRRARRDWVTVLGLTCLLGTTWGTAFCAFGVLLVPQLYLFTILNSLQGQHPGGILGEEGVPSSSRRGTEGAHRTNCVTPQVCSSAFGTSARIAASAAHPTAAPPDSGAAGSASVFSVN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The presence of G-protein coupled receptors family 2 profile, GPS domains is critical for the interaction of the designed protein with its intended targets.3. The ideal protein would be one that plays a critical role in negative regulation of cell population proliferation, positive regulation of cell adhesion, hematopoietic stem cell homeostasis through G protein-coupled receptor activity within extracellular region.4. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "LAMIHSIEMINNSTLLPGVKLGYEIYDTCTEVTVAMAATLRFLSKFNCSRETVEFKCDYSSYMPRVKAVIGSGYSEITMAVSRMLNLQLMPQVGYESTAEILSDKIRFPSFLRTVPSDFHQIKAMAHLIQKSGWNWIGIITTDDDYGRLALNTFIIQAEANNVCIAFKEVLPAFLSDNTIEVRINRTLKKIILEAQVNVIVVFLRQFHVFDLFNKAIEMNINKMWIASDNWSTATKITTIPNVKKIGKVVGFAFRRGNISSFHSFLQNLHLLPSDSHKLLHEYAMHLSACAYVKDTDLSQCIFNHSQRTLAYKANKAIERNFVMRNDFLWDYAEPGLIHSIQLAVFALGYAIRDLCQARDCQNPNAFQPWELLGVLKNVTFTDGWNSFHFDAHGDLNTGYDVVLWYQ",
    "response#2": "LAMIHSIEMINNSTLLPGVKLGYEIYDTCTEVTVAMAATLRFLSKFNCSRETVEFKCDYSSYMPRVKAVIGSGYSEITMAVSRMLNLQLMPQVGYESTAEILSDKIRFPSFLRTVPSDFHQIKAMAHLIQKSGWNWIGIITTDDDYGRLALNTFIIQAEANNVCIAFKEVLPAFLSDNTIEVRINRTLKKIILEAQVNVIVVFLRQFHVFDLFNKAIEMNINKMWIASDNWSTATKITTIPNVKKIGKVVGFAFRRGNISSFHSFLQNLHLLPSDSHKLLHEYAMHLSACAYVKDTDLSQCIFNHSQRTLAYKANKAIERNFVMRNDFLWDYAEPGLIHSIQLAVFALGYAIRDLCQARDCQNPNAFQPWELLGVLKNVTFTDGWNSFHFDAHGDLNTGYDVVLWYS",
    "response#3": "TLRGSFSSAAARDAQGQSIGHFEFHGDHALLCVRINNVAVAVGKEAKLYLFQAQEWLKLLESSPGYSCSERLARAQLTVTVTQTEHNLTVSQLPAPQTWRVFYADKFTCS"
  },
  {
    "reference": "MLKGKKILLGVTGSIAAYKAAEIVSLLKKKGAEVFVIMTESATKFVQPLTFSTLSENPVIDNLFSPGDKITVKHISLAEWADLILIAPATANIVGKIAQGIADDMLTTTVMASQAKVFFAPAMNKNMLSNPLYLQNVEKLSALRYEFIDSEYGRLACGETGEGRLANIEDIVDTVEYALTFKNDYRGKTVLVTASCTREPIDKVRFISNYSSGKMGFALARIARARGARVILITGPTSLPDPKGVTTVSIESAEEMKKAVFKYFPEADIVISAAAVADFRPKEVFAGKLKKEKSEKPLIELERTPDILYELGKKKGDKILVGFAAETENFAANALKKLKEKSLDLIVANDISAKDAGFGSDKNRARLINSKGTARELPLMPKIDMAEKILDEILKIK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that is capable of catalyzing reactions within the CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis, coenzyme A biosynthesis pathway with high efficiency and specificity.2. The designed protein should have phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity, metal ion binding, FMN binding to facilitate coenzyme A biosynthetic process, pantothenate catabolic process.3. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.4. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.5. The presence of DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.6. The protein should have a high affinity for CTP.7. The protein with the reaction H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2, the Proton donor must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALAVARALARPPGDLLRGLKVVVSAGPTYEDIDPVRFIGNRSSGRMGFAVAEAAAQAGAEVTLVAGPVSIATPPGIARRIDVRSAAQMRDAVVTASAGADIYIAAAAVGDYRPAEVADHKLKKSDGAPLELRLTENPDILVTLAAQTVRPFLVGFAAETHDVERYAQDKLKRKGLDMIAANQVGGGLGFEAADNALTVAAADGPERWERAPKAAIAGRLAAEIARRLAA",
    "response#2": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALGLAGLLLRPADLLLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHLVDADGREVALAQGSKRVVAGAIWDTILGRL",
    "response#3": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALALLEPLLEPRDLRGLKVVVSAGPTYEDIDPVRFIGNRSSGRMGFAVAEAAAQAGAEVTLVAGPVSIATPPGIARRIDVRSAAQMRDAVVTASAGADIYIAAAAVGDYRPAEVADHKLKKSDGAPLELRLTENPDILVTLAAQTVRPFLVGFAAETHDVERYAQDKLKRKGLDMIAANQVGGGLGFEAADNALTLVDAAGHTELPRASKLALASALVDEVAARLPR"
  },
  {
    "reference": "MADSGPAGGAALAAPAPGPGSGSTGPRVYFQSPPGAAGEGPGGADDDGPVRRQGKVTVKYDRKELRKRLNLEEWILEQLTRLYDCQEEEIPELEIDVDELLDMESDDTRAARVKELLVDCYKPTEAFISGLLDKIRGMQKLSTPQKK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must possess phosphatase inhibitor activity, protein serine/threonine phosphatase inhibitor activity to support innate immune response under certain conditions. The designed protein sequence is ",
    "response#1": "MNESKRENIQVINIGDCRAVLCKNGLAIPLNKDHKPIWPDEKRRIDRVNEKYETNEKIHFDAGDWRIGDLSVSRSFGDLDNTPYVTHVPDLFDYQLQSDDEFIIMACDGVWDVLENHEAINFVRDHRNDNHTEFYSIPGKYPNREAFESDNISRKLASYAIARGSTDNVSVIIIFQ",
    "response#2": "MNESKRENIQVINIGDCRAVLCKNGLAIPLNKDHKPIWPDEKRRIDRVNEKYETNEKIHFDAGDWRIGDLSVSRSFGDLDNTPYVTHVPDLFDYQLQSDDEFIIMACDGVWDVLENHEAINFVRDHRNDNHTEFYSIPGKYPNREAFESDNISRKLASYAIARGSTDNVSVIIIFS",
    "response#3": "DQLSAAPGVKGMVMTVFTLPSFPYVFKVIKDRLGGTKEFGRQTVIDRYRMVKRHDRVGRMADTLEFVDVALPLKRISADLLDEFKQTIANSISIEGDTLVIHQLFVERRMTPLNLYLEYANDEEVDAAMDDYGRALKEMMAANIFPGDMLLKNFGVTRHKRVVFYDYDEVRYLTDMSFRRLPENDWEVSYAPDDVFPQQLAQFAVPQAKYRNKLLKRHPELIDSAYWCRVQKNIRKGELTDVFPYD"
  },
  {
    "reference": "MTTVVSRNAKQMKNAYGFSAQHVKLTPAQRKRACYCEDDGLVHYHCTQCDLICDSLTKARPVNHDCDGEPFETFDDDISAPIYTTTLKDFLSNDDFGRLAKSNSGPQEEITDDIAVIKVSQPFCNVMEVTETKLEELEGKVGEITKTGVNHNMSFSSCVTPVNSKCNDGTDEHQFIKFGSFEPIEIKPVQKIVSNERQFVKFGSFEPVAVEQGPIKFNTLGAHVRLDRPTGKVPQLEKRTNLTKVKTPTRAHIVKVTPKQNPLPTANKGVPFQRYKKVWVKKETSKLPTPIVTNGAPAYVKPNEKPLFVKPLYKRKNMKHERASIDNLAVPLDALCDKLLDIVHERAISLTVIGKTKYKFCSVELNGKRYYKIVTKHENGVIGRLDMKNDAETLSILRYFLKRNPDDLIDEFDIQKGHSGVIINPDNIIGRKPLTYETDVMVVRGRLYGRLVDSLLKVHKNKIHDIEHYSNNVQAVINSPETFEDITQESIRLFGEIESLSGNRATSQLTQINVIAQSLLKGQVMSSIEQEAAFSNLKALALWYRKKLEAQQTGDITTFRNKISAKTHINLALMCDNQLDVNGIFQWGERGYHAKRFFEKYFTRIDGGAQYEHFSIRKHIRGSRELAIRNLIVSTDIDKMTQSMRGSPIADVELGEYCVSRFSKNFVYPCCCVTHESGAAMRSEFKFPTKNHLVIGNSGDDKSVELPAREDGNMYIVKDGYCYILIFLAMMINIREGDAKAFTKRVRDFIITKLGEWPRMRDLAILCR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be able to perform RNA binding under specific conditions. The designed protein sequence is ",
    "response#1": "GRSCLYVQTPDTRVLIDCGINVACEDKAFPHFDAPEFSIEDLDAVIVTHAHLDHCGFIPGLFRYGYDGPVYCTRPTRDLMTLLQKDYLEIAKKEGKEVPYTSKDIKTCVKHTIPIDYGVTTDISPTIKLTLHNAGHVLGSAIAHLHIGEGLYNLAYTGDIKFETSRLLEPAVCQFPRLETLIIESTYGAYDDVLPEW",
    "response#2": "FQTTVTAQILLKALTNLPHTDFTLCKCMIDQTHQEERPIRQILYLGNLLETCHFQSFWASLEENRDLIDGITGFEESVRKFICHVVGITYQNIEYRLLAEMLGDPLDTQVKVWMNKYGWTENEDGQIFIHNQEESVKPKL",
    "response#3": "FQTTVTAQILLKALTNLPHTDFTLCKCMIDQTHQEERPIRQILYLGNLLETCHFQSFWASLEENRDLIDGITGFEESVRKFICHVVGITYQNIEYRLLAEMLGDPLDTQVKVWMNKYGWTENEDGQIFIHNQEESVKPKS"
  },
  {
    "reference": "MPTGFVAPILCVLLPSPTREAATVASATGDSASERESAAPAAAPTAEAPPPSVVTRPEPQALPSPAIRAPLPDLYPFGTMRGGGFGDRDRDRDRGGFGARGGGGLPPKKFGNPGERLRKKKWDLSELPKFEKNFYVEHPEVARLTPYEVDELRRKKEITVRGGDVCPKPVFAFHHANFPQYVMDVLMDQHFTEPTPIQCQGFPLALSGRDMVGIAQTGSGKTLAYLLPAIVHINHQPYLERGDGPICLVLAPTRELAQQVQQVADDYGKCSRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLESGKTNLRRCTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLRDYTQINVGNLELSANHNILQIVDVCMESEKDHKLIQLMEEIMAEKENKTIIFVETKRRCDDLTRRMRRDGWPAMCIHGDKSQPERDWVLNEFRSGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYVHRIGRTARSTNKGTAYTFFTPGNLKQARELIKVLEEANQAINPKLMQLVDHRGGGGGGGGRSRYRTTSSANNPNLMYQDECDRRLRGVKDGGRRDSASYRDRSETDRAGYANGSGYGSPNSAFGAQAGQYTYGQGTYGAAAYGTSSYTAQEYGAGTYGASSTTSTGRSSQSSSQQFSGIGRSGQQPQPLMSQQFAQPPGATNMIGYMGQTAYQYPPPPPPPPPSRK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Contrary to splicing regulation activity, transcriptional coregulation of the estrogen receptor ESR1 is helicase-independent; Mediates the use of alternative promoters in estrogen-responsive genes and regulates transcription and splicing of a large number of steroid hormone target genes; Plays a role in estrogen and testosterone signaling pathway at several levels; Plays a role in innate immunity; In addition to binding mature mRNAs, also interacts with certain pri-microRNAs, including MIR663/miR-663a, MIR99B/miR-99b, and MIR6087/miR-6087; Coactivates MMP7 transcription.2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in nucleus, nucleolus, cytosol that is critical for RNA processing, regulation of skeletal muscle cell differentiation, positive regulation of transcription by RNA polymerase II.3. The presence of Helicase ATP-binding domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "VMCYNVLCDKYATRQLYGYCPSWALNWDYRKKAIIQEILSCNADIVSLQEVETEQYYSFFLVELKERGYNGFFSPKSRARTMSEQERKHVDGCAIFFKTEKFTLVQKHTVEFNQLAMANSEGSEAMLNRVMTKDNIGVAVLLELRKESIEMPSGKPHLGTEKQLILVANAHMHWDPEYSDVKLVQTMMFLSEVKNIIDKASRNLKSSVLGEFGTIPLVLCADLNSLPDSGVVEYLSTGGVETNHKDFKELRYNESLTNFSCHGKNGTTNGRITHGFKLQSAYESGLMPYTNYTFDFKGIIDYIFYSKPQLNTLGILGPLDHHWLVENNISGCPHPLIPSDHFSLFAQLEHQKQALAWMVSRENSKELPPFWEQRNDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNFHDGRPLPIERVKKNLLKKEYNVNDDSMKLGGNNTSEKADGLSKDASRCSEQPSISDIKEKSKFRMSELSSSRPKRRKTAVQYIESSDSEEIETSELPQKMKGKLKNVQSETKGRAKAGSSKVIEDVAFACALTSSVPTTKKKMLKKGACAVEGSKKTDVEERPRTTLIICPLSVLSNWIDQFGQHIKSDVHLNFYVYYGPDRIREPALLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIRNPNAQQTKAVLDLESERRWVLTGTPIQNSLKDLWSLLSFLKLKPFIDREWWHRTIQRPVTMGDEGGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGRATIGRYFNEGTVLAHYADVLGLLLRLRQICCHTY",
    "response#2": "VMCYNVLCDKYATRQLYGYCPSWALNWDYRKKAIIQEILSCNADIVSLQEVETEQYYSFFLVELKERGYNGFFSPKSRARTMSEQERKHVDGCAIFFKTEKFTLVQKHTVEFNQLAMANSEGSEAMLNRVMTKDNIGVAVLLELRKESIEMPSGKPHLGTEKQLILVANAHMHWDPEYSDVKLVQTMMFLSEVKNIIDKASRNLKSSVLGEFGTIPLVLCADLNSLPDSGVVEYLSTGGVETNHKDFKELRYNESLTNFSCHGKNGTTNGRITHGFKLQSAYESGLMPYTNYTFDFKGIIDYIFYSKPQLNTLGILGPLDHHWLVENNISGCPHPLIPSDHFSLFAQLEHQKQALAWMVSRENSKELPPFWEQRNDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNFHDGRPLPIERVKKNLLKKEYNVNDDSMKLGGNNTSEKADGLSKDASRCSEQPSISDIKEKSKFRMSELSSSRPKRRKTAVQYIESSDSEEIETSELPQKMKGKLKNVQSETKGRAKAGSSKVIEDVAFACALTSSVPTTKKKMLKKGACAVEGSKKTDVEERPRTTLIICPLSVLSNWIDQFGQHIKSDVHLNFYVYYGPDRIREPALLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIRNPNAQQTKAVLDLESERRWVLTGTPIQNSLKDLWSLLSFLKLKPFIDREWWHRTIQRPVTMGDEGGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGRATIGRYFNEGTVLAHYADVLGLLLRLRQICCHTY",
    "response#3": "FKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPYGCLLDYIREHKDNIGSQYLLNWCVQIAKGMSYLEERRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSVLEKGERLPQPPICTIDVYMIMVKCWMIDAESRPKFRELIIEHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLYLLQNVVRPQDQTVVFVATKHHAEYLTELLMGQGVSCAHIYSALDQTARKINLAKFTHNKCSTLIVTDLAARGLDIPLLDNVINYSFPAKGKLFLHRVGRVARAGRSGTAYSLVAPDEVPYLLDLHLFLGRSVTLARPCEEPSVA"
  },
  {
    "reference": "MRITQGTFSFLPDLTAAQVKAQIQYALDQNWAVSVEYTDDPHPRNTYWEMWGLPMFDLRDAAGVYGEVEACRTAHPGKYVRVNAFDSNRGWETVRLSFIVQRPEKEDGFRLDRTEGPGRTQRYALQHRSYAAG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have ribulose-bisphosphate carboxylase activity to facilitate reductive pentose-phosphate cycle.2. For general function, the protein need meet that RuBisCO catalyzes two reactions: the carboxylation of D- ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate The designed protein sequence is ",
    "response#1": "MFETFSYLPPLTDEQISKQVDYILANAWTPCLEFAASDQAYAGNENCITMGPVASTYQDNRYWTMWKLPMFGCTDGSQVLTEIQACTKAFPDAYIRLVCFDANRQVQISGFLVHRR",
    "response#2": "MFETFSYLPPLTDEQISKQVDYILANAWTPCLEFAASDQAYAGNENCITMGPVASTYQDNRYWTMWKLPMFGCTDGSQVLTEIQACTKAFPDAYIRLVCFDANRQVQISGFLVHRR",
    "response#3": "MFETFSYLPPLTDEQISKQVDYILANAWTPCLEFAASDQAYAGNENCITMGPVASTYQDNRYWTMWKLPMFGCTDGSQVLTEIQACTKAFPDAYIRLVCFDANRQVQISGFLVHRR"
  },
  {
    "reference": "MNHIRELQMKPEDFDRVKVIGRGAFGEVQLVRHRASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFADSPWVVQLCCAFQDDRYLYMVMEYMPGGDLVNLTSTYDVPEKWAKFYTAEVVMALDAIHSMGFIHRDVKPDNMLLDRNGHLKLADFGTCMKMDSTGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFIFEMLVGDTPFYADSLVGTYSKIMDHKNSLNFPDDVEISKEAKNIICAFLTDREVRLGRNGVGEIKSHPFFKNDQWTFDTIRDTAAPVVPELSSDIDTSNFDEIEEDKGDVETFPTPKAFVGNQLPFVGFTYFKEDQLLTSSNSSAEHPTKGEIQKKLHCLEEQLNNEMQAKDELEHKYRTTSGRLDKITKELEEEINSRKALESSLRQLEREKALLQHKSVESHRKAESEADRKRCLENEVNSLRDQLEEMKKRNQNSHISNEKNIQLQRQLDEANALLRAESDAATRLRKSQTESSKQLQQLETHVRELQDKCCLLESSKLALEKDYISLQAALDTETREHTQRSETISDLQVRQSSLEDEVKQVRQALAKAESEKRQLQEKLTDLEKEKSNKEIDMTYKLKVLQQSLEQEEGAHKATKARLADKSKISESIEGAKSEAMKELEWKLQEERSSKLRVENRLLELEKQNSMLDCDYKQALQKLDELRRHKDRLTEEVKNLSLRIEQEMQKRSLTQNDLKVQNQQLSTLRTSEKQLKQEINHLLDIKRSLEKQNTELRKERQD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The ATP binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must exhibit the following characteristics: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity..3. Design the protein with a signal peptide incorporated.4. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.5. The presence of AGC-kinase C-terminal, PH domains is critical for the interaction of the designed protein with its intended targets.6. The designed protein should have protein serine/threonine kinase activity, ATP binding within plasma membrane to regulate cell development, regulation of actin cytoskeleton organization, Rho protein signal transduction.7. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.8. The protein should exhibit Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "INCGETDVPFDNHGRTWTQEEKNILPKNSDNASFSSVVSYKEESGIPQVPYMTARIFRSDFTYSFPVSPGWKFLRLYFYPTSYKSGFDAVNSFVSVTVNDFTLLQNFSADLTVKASIPESKSLIKEFIVPVYLTLNLTFRPSNNSLAFVNGIEIVSMPDRFYSKGGFDDLITNVGSLIDFEIDNSTASETVHRLNVGGHMVDEVNDSGMFRRWLSDDYEFLIGGVSPYMPDVNISYTEKTPAYVAPAYVYSTCRMMGNAQDTYLNLNFNLTWLFTVDAGFSYLVRLHFFEKYLNKANQRVFSIFLGNQMAREEMDVIRLSGGPRIPIYLDFRIYVGSESGPRPDLRLDLHPLVKDNPEYYEAILNGVEIGARPCLRSKLVVLGDPGVGKTSVIQCIKGKKKKQTNNNVNINNNGSEIGIEIDQYDFDIVDEDDKKRRVITLSTWDIANQDVYFSSSQLFDSERSVYIVVFSLNNDDFSAIEYWLHCIMSTSPNSPIVLVGTHIDEFENLNVVNAVLESVASRFQKRFSNIQAIISVSCTTGYDVDKLRQLIEEIIKAPEEEKPPYTPSSFFTLEEALIEVKKKRIPPVMMWQEYINLANICNLKDSVQIQRATEFLHNIGSIVYFNDLNSSVGKMVILDQQWIINCMSSLITSKVLINNCNGIVRQSDLELVWKAPTYPEHLHSALLSIMQAFEICRPLSPSDLVKPDEINDKEVIGDRNLVPNLLSDNNSNIITQWDDFIDPDTILLNRQYHLPFLPEKFFGKLIIHLMNFTKVESCYKRVV",
    "response#2": "YEVVKVIGRGAFGEVQLVRHKATRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSSWVVQLFFAFQDDRYLYMVMEYMPGGDLVNLMSNFDVPEKWARFYTAEVVLALDGIHSMGFIHRDVKPDNMLLDKTGHLKLADFGTCMKMKEDGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNALTFPDDSDISNDAKNLICAFLTDREVRLGRNGVDEIKRHRFPSSFFTLEEALIEVKKKRIPPVMMWQEYINLANICNLKDSVQIQRATEFLHNIGSIVYFNDLNSSVGKMVILDQQWIINCMSSLITSKVLINNCNGIVRQSDLELVWKAPTYPEHLHSALLSIMQAFEICRPLSPSDLVKPDEINDKEVIGDRNLVPNLLSDNNSNIITQWDDFIDPDTILLNRQYHLPFLPEKFFGKLIIHLMNFTKVESCYKRVVPEVQVQQAYLNVSNVLRCIALGYPAPEITWYRCSGNKDSCNQDRVLLWHDANTSVLSQEPFHKVTVESIFHIQTLMENTTYMCMASGGDQWTWENIRDTAAPVVPELSSDTDTSNFDDIEEDRGEEETFPIPKAFVGNQLPFVGFTYYSNQDLLRCPEVQVQQAYLNVSNVLRCIALGYPAPEITWYRCSGNKDSCNQDRVLLWHDANTSVLSQEPFHKVTVESIFHIQTLMENTTYMCMASGGQQCSFTVETEPEVQVQQAYLNVSNVLRCIALGYPAPEITWYRCSGNKDSCNQDRVLLWHDANTSVLSQEPFHKVTVESIFHIQTLMENTTYMCMAS",
    "response#3": "YEVVKVIGRGAFGEVQLVRHKATRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDGIHSMGFIHRDVKPDNMLLDKAGHLKLADFGTCMKMNQDGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNALTFPDDSEISKDAKNLICAFLTDREVRLGRNGVDEIKRHPFFDQWTWENIRDTAAPVVPELSSDTDTSNFDDIEEDRGEEETFPIPKAFVGNQLPFVGFTYYSNQDLLRCAPSSFFTLEEALIEVKKKRIPPVMMWQEYINLANICNLKDSVQIQRATEFLHNIGSIVYFNDLNSSVGKMVILDQQWIINCMSSLITSKVLINNCNGIVRQSDLELVWKAPTYPEHLHSALLSIMQAFEICRPLSPSDLVKPDEINDKEVIGDRNLVPNLLSDNNSNIITQWDDFIDPDTILLNRQYHLPFLPEKFFGKLIIHLMNFTKVESCYKRVVGARPCLRSKLVVLGDPGVGKTSVIQCIKGKKKKQTNNNVNINNNGSEIGIEIDQYDFDIVDEDDKKRRVITLSTWDIANQDVYFSSSQLFDSERSVYIVVFSLNNDDFSAIEYWLHCIMSTSPNSPIVLVGTHIDEFENLNVVNAVLESVASRFQKRFSNIQAIISVSCTTGYDVDKLRQLIEEIIKA"
  },
  {
    "reference": "MRGPPHAHPPSRWLRHAVSPSRVGTASPADNPMAHAGARLATHLPRLRHRCRTRHGGPHPGRPRTARCRRHRPRSHADGDGLALRDLGRRQNGGKRAAGGRRRHPRCPGQFFFLRYPVMTTTTVPELAVPEFQHRTVLLDEAVDALALSGARANGVFVDGTFGRGGHSRLILSRLGASARLIAFDKDPQAIATAEQIDDPRFTIVHDSFATMRTALAERGVGQADGILLDLGISSPQVDDAARGFSFRNDGPLDMRMDTTRGMSAAQWLAIENEQTLEKVIKEYGEERFAFQIAKAIVARRAVEPISTTRQLAALVAGAVKTREKGKDPATRTFQAIRIFINKELEDLEIGLTEAYASLAPGGIMAIISFHSLEDRMVKRFFASKANVEQPDRRLPIRARERQDQRRAGHAAGGLRPGAGQRPVPGAPPVHRTGTHAVAGAPARHRMGPAAAGPVDAGQARTHRRNRPGRPGHDAAHRGAHAEPGAGGAPARLAFARGAVRVVLRLCRAGRTRHVAAGRLHPVPAKAGRDPLRPHARIARHARQDHRPRWPGAGVVGARQGHLGDSGRRPERARGKTAPAGQAARHEQRRPAEKTRFRPRLRVPETPGGAGRLRADHEARYRRRLHPQGIQALLPAGRSDHPRGGLHQRGRRGPGIDGTGAAKKPGRPAGQPPRDQGPPGPHRRRHRIDPRAARRQGPHAVHRQQDPVHRLHPDQGSGREVQGQGRRRRGAGRAHGRGAGAGQLPDLQPQRPLQAHGRAIAQPRDDRH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein contains novel ATP-grasp domains that confer a unique function or activity.2. A protein that contributes to biosynthetic process, cellular response to oxidative stress, cell division, cell wall organization by functioning in plasma membrane with metal ion binding, thioredoxin peroxidase activity.3. The protein design should emphasize Basic residues, Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "ITAVDCGIKYNQIRCLCQRGARVTIVPWNQPLDSADFDGLFISNGPGDPQFCKETVENIKKVLCEENPKPVFGICLGHQLLSLAIGAKTYKMKYGNRGHNQPCIHKGTGRCFITSQNHGFAVDPHTLPSDWDVLFTNANDQTSEGIVHNTKPMFSVQFHPEHMAGPTDLVGLFDVFINAMIAAKTQ",
    "response#2": "ITAVDCGIKYNQIRCLCQRGARVTIVPWNQPLDSADFDGLFISNGPGDPQFCKETVENIKKVLCEENPKPVFGICLGHQLLSLAIGAKTYKMKYGNRGHNQPCIHKGTGRCFITSQNHGFAVDPHTLPSDWDVLFTNANDQTSEGIVHNTKPMFSVQFHPEHMAGPTDLVGLFDVFLEQIQ",
    "response#3": "ITAVDCGIKYNQIRCLCQRGARVTIVPWNQPLDSADFDGLFISNGPGDPQFCKETVENIKKVLCEENPKPVFGICLGHQLLSLAIGAKTYKMKYGNRGHNQPCIHKGTGRCFITSQNHGFAVDPHTLPSDWDVLFTNANDQTSEGIVHNTKPMFSVQFHPEHMAGPTDLVGLFDVFLDVTL"
  },
  {
    "reference": "MVRWKKPKRFLCRKTQDYERIDDIENNDEDSDDDVILEINHDGEHEDNYRNPMIRMAYIPIVIIAVIYSSFIFFFALFIEASAHTNSKCSEAHEKEKMSSGLLTPPMKHILEDRTKYKNEIKLLKTFNVHLTYSTWINSILEILALIYSFLLLLVDRQYLYPLSQVILYLFTRINFVYTDIYGRFFSPSDVVGAITTEVIYDLIRWQSESKLFKVFPFTPCPLPLLLFPIFLSILQVFANQKHTHVYETVLLIFFRIMTRFAGRRPFILSYQVLRSGIASFQSATSHDIAKTLNKVTHCCLNVFASSAFFVLLMVLVDKQGLDKTPKCLAALYAFAMWMQLATVRYRHFIPLILTVVIELVITGLVSYQTGEFIFSHTAEVKDYVFTVMFTIIAVFRFVFIIILFKVLIYKNPPHSLTTTVLLKPTNIPVTFSSIKAPQELDPTNPYYPQTVYNSNQSKNNLLPLTRD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should contain a Helical membrane-spanning region.2. The designed protein localizes to the membrane. The designed protein sequence is ",
    "response#1": "IPAEVVLPLAGYWVSKGDMTLAGVVLAGSLGGVAGPLTLYWIGRYGGRPFLERFGKYLFIKPEALDKSDNFFKKHGGFVAFSGRFLPGIRTLISIPCGIAKMNVWVFSLYTFIAMLPITFVYVYLGD",
    "response#2": "IPAEVVLPLAGYWVSKGDMTLAGVVLAGSLGGVAGPLTLYWIGRYGGRPFLERFGKYLFIKPEALDKSDNFFKKHGGFVAFSGRFLPGIRTLISIPCGIAKMNVWVFSLYTFIAMLPITFVYVYLGA",
    "response#3": "IPAEVVLPLAGYWVSKGDMTLAGVVLAGSLGGVAGPLTLYWIGRYGGRPFLERFGKYLFIKPEALDKSDNFFKKHGGFVAFSGRFLPGIRTLISIPCGIAKMNVWVFSLYTFIAMLPITFVYVYLGS"
  },
  {
    "reference": "MAAASALSRGALDVIFNDPDKAAKLFPVPVLQCLQVKQMAPSAQGGERYRLVMSDGQHYVQTMLATQANHVVRDNKLVRGCFARIKQYTPNNLKGKNILVILDLEVVESLGIQDKIGEPVAVDAKPPGQEGAIAGGDFYGVKKEEQKPQPQQFQQQQQQQQSMPSRPAMHGGSNIYPIEGLSPFAHKWTIKARVTSKSDIKTWHKATGEGKLFSVNLLDESGEIKATGFNEQCDAFYDRLQEGSVYYISTPCRVSLAKKQFSNLPNDYELAFERDTVIEKAEDQTNVPQVRFNFCTIQELQSVEKDNTVDVIGVLKEVGEIGEIVSKKDGRPFQKRELTLVDDTGYSVRVTVWGKTANSFDAPAESVVAFKGTKVSDFGGKSLSLLSSGTMSVDPDIADAHRLKGWYDSAGRTDTFATHQGMASMGNATGRKDEIKTISQVKDENLGMDDQAYYTIKATIVFMKQDTFCYPACASQGCNKKVTEMGDGTWHCEKCNVSHEKPEYRYILSLNVADHTSHQWLSCFDDSGQKILGRTANEMMELKESDDPTKFTAAFEEANCKKLTFRCRAKMDNFGEAQRIRYQVMSAMPLDFTSEGNKLKEMIKEYENMGM",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have double-stranded DNA binding, single-stranded DNA binding, metal ion binding within chromatin to regulate protein ubiquitination, telomere maintenance via recombination, DNA unwinding involved in DNA replication.2. The protein must exhibit the following characteristics: Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism.; As part of the replication protein A (RPA/RP-A), a single- stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair.3. The protein design should emphasize Polar residues compositional bias.4. The designed protein should contain one or more Replication factor A C-terminal, Replication factor-A protein 1 N-terminal, OB domains that are essential for its function. The designed protein sequence is ",
    "response#1": "IAQVKDENLGMDDQAYYTIKATIVFVKQENFCYAACLSQGCNKKVTQMPDGTWQCEKCNLSHEKPEYRYILSLNVADHTSHQWLTGFDDFGRQVMGRTADEMMELKENDDTKLTAAFEEANCKKFTFRCRAKMDNFGEAQRIRYQVMSVTPLDFKSEGTKLDELIKYFSIKASVTFCKPENFAYPACPNLVQNADATRPAQVCNKKLVFQDNDGTWRCERCAKTYEEPTWRYVLSCSVTDSTGHMWVTLFNDQAEKLLGIDATELVKKKEQKSEVANQIMNNTLFKEFSLRVKAKQETYNDELKTRYSAAGINELDYASESQFYFSCIATIVYIRKENCLYQACPSKDCNKKVVDQQNGMFRCEKCDKEFPDFKYRLMLSANIADFGDNQWVTCFQDTAETLLGQNSSYLGQLKDTNEAAFDEVFQHANFNTFVFRNRVKLETYNDESRIKVTVVDAKPVDHREYSKRWTIRAKVISKNDIKTWNNARGSGKLFSTVFMDNSSEIRATAFNDACDKFYDLLQEDQVYYISGANIKVANKQFNNVKNEYEMLVSEFLSHITSNPSLGEEKPVFFSTRAYISFIKPDQTMWYRACKTCNKKVTEAMDSGYWCESCQKKDQECSLRYIMAVKVSDSTGETWLSAFNDEAEKIIGCTADDLNDLKSEEGEVNEFQTKLKEATWSSHLFRISVSQQEYNSEKRQRITVRGVSPIDFAAETRLLLQDISA",
    "response#2": "INQALEEHSGSTEKPDYFSIKASVTFCKPENFAYPACPNLVQNADATRPAQVCNKKLVFQDNDGTWRCERCAKTYEEPTWRYVLSCSVTDSTGHMWVTLFNDQAEKLLGIDATELVKKKEQKSEVANQIMNNTLFKEFSLRVKAKQETYNDELKTRYSAAGINELDYASESQFLIKKLDVAQVKEEQLGMSDEVAYFSLKATVIYIKQDTMCYPACLSEGCNKKVTELDPGQWRCERCDKTHPRPEYRYIMLISVSDHTGQLYLSCFDEVGRYMMGTSADQLMEIRQNDDKAAGDIFQDANCRTWNFRCRAKIDNFGDQQRIRCQIVTAKPVNYSEEALRLANMIDLDQVQNLEANAIIDVLGALKTVFPPFQITAKSTGKVFDRRNILVVDETGFGIELGLWNNTATDFNIEEGTVVAVKGCKVSDYDGRTLSLTQAGSIIWTIRAKVISKNDIKTWNNARGSGKLFSTVFMDNSSEIRATAFNDACDKFYDLLQEDQVYYISGANIKVANKQFNNVKNEYEML",
    "response#3": "IAQVREEQLGMSEEPSYFTLRATVVYIKQDNLCYPACLSEGCNKKVTEVDPGQWRCERCDKTHPRAEYRYIMLVNVSDHTGQLYLNCFDDVGRLLLGMSANQLMELPQNDGKALGNVIQNATCRTWTFGCRAKIDHYGDQQRIRYQVSYAKPVNYSEEASRLADIIDINQALEEHSGSTEKPDYFSIKASVTFCKPENFAYPACPNLVQNADATRPAQVCNKKLVFQDNDGTWRCERCAKTYEEPTWRYVLSCSVTDSTGHMWVTLFNDQAEKLLGIDATELVKKKEQKSEVANQIMNNTLFKEFSLRVKAKQETYNDELKTRYSAAGINELDYASESQFLIKKLDS"
  },
  {
    "reference": "MLIVLSVGGSILAKNLDPKHFSAYASALKELARKHQIVVVTGGGVAARNYIDVARGVGANEVVCDFIGIDITRLNAQLLIAALGNTAHPEPPTTYKEAESALASGKIVVMGGVIPGQTTDAVAAILAEYLNADMMVIATSVDGVYSSDPREDPNAKKFDVMTAKELVGIVISTEMKAGSKSPVDPLASKIIERCNIDTIIMDGTDPQDVLEVVLQEAVKTDKVTGVRLGTRIIG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. We need a protein that can perform ATP + UMP = ADP + UDP with high efficiency.2. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.3. The designed protein should have ATP binding to facilitate phosphorylation. The designed protein sequence is ",
    "response#1": "LEEPLGRGATGTVWRARQRETAGAEAAVAGQPGETVAIKVLKEELASDADIVMRFLRERSVLLRLTHPNIVRVRDLVVEGELLALVMDLIDGPDLHRYLRENGPLTPVAAALLTAQIADALAASHADGVVHRDLKPANVLLKQTGGEMHPMLTDFGIARLADSPGLTRTHEFVGTPAYVAPESAEGRPQTSAVDVYGAGILLYELVTGRPPFGGGSALEVLHQHLSAEPRRPSTVPDPLWTVIERCLRKNPDDRPSAENL",
    "response#2": "PSEAPVEKYTQTNLLSNILFKDVHYEVDEKARNIILTDKGTLFCEDHLSIDNLYDLENPWVHYILNAIKAKELFIKDVHYIIRDNQVVIVDEFTGRIMSGRRWSDGLHQAIV",
    "response#3": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYL"
  },
  {
    "reference": "MSSKSHLPYAIRATNHPNPLTSKLFSIAEEKKTNVTVSADVTTSAELLDLADRLGPYIAVLKTHIDILTDLTPSTLSSLQSLATKHNFLIFEDRKFIDIGNTVQKQYHGGALRISEWAHIINCAILPGEGIVEALAQTTKSPDFKDANQRGLLILAEMTSKGSLATGEYTARSVEYARKYKGFVMGFVSTRALSEVLPEQKEESEDFVVFTTGVNLSDKGDKLGQQYQTPGSAVGRGADFIIAGRGIYKADDPVEAVQRYREEGWKAYEKRVGL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be able to bind substrate ligand in a variety of conditions2. A protein that contributes to sporocarp development involved in sexual reproduction, 'de novo' UMP biosynthetic process, 'de novo' pyrimidine nucleobase biosynthetic process by functioning in cytosol with orotidine-5'-phosphate decarboxylase activity.3. The protein with the reaction H(+) + orotidine 5'-phosphate = CO2 + UMP, the Proton donor must be optimized to ensure efficient catalysis.4. Ideal protein should have a binding affinity for key enzymes in the UMP biosynthesis via de novo pathway, UMP from orotate: step 2/2, Pyrimidine metabolism pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MKLCIALDNPSKEENLKLAKELKEYAKELWLKVGFRSYIRDGAEFIKELKLMGYNVFLDLKLYDIPNTMADAAEEIANLGVDMFNVHASAGSIALKTVMQRLEKYEKRPLVLAVTALTSFTDEEFKKIYGDSIENKAKEFAKLSHEAGLDGVVCSAFESKMIKNITDEKFITLTPGIRPFGEDAGDQARVADISLAKEQKVDFIVVGRPIYKDEDPKAKVEKL",
    "response#2": "VGLDPDPDRLPEFLDADLPRWAFNRRVIDATHEHAACYKPNAAFYEDSDGWRALEETIAYAHGKGVPVLLDAKRGDIGNTARQYATLLDDDGLGADAITANPYMGRDTLEPYLSRADKGVFILCRTSNSGGADFQNLTVGNDKRLYEYVAQRCQEWNEHENVGLVVGATAPEELESVRDLVDLPFLVPGVGAQGGDAAAAVDHGLADGVGLVNSSRGIIFAGEGNLCASLDVTTTSELLKLVDTLGPYICLLKTHVDILSDFSFENTVKPLKEMAAKHNFLIFEDRKFADIGNTVKLQYTSGVYKIAEWADITNAHGVTGQGIVTGLKQGAEETTNEPRGLLMLAELSSKGSLAHGEYTKGTVDIAKSDKDFVIGFIAQKDMGGRDEGFDWLIMTPGVGLDDKGDALGQQYRTVDEVFSTGTDIIIVGRGLFAKGRDPKTEGENLCASLDVNTTEELLKLVDTLGPYICLLKTHIDIISDFSIDGTVKPLKELAKKHNFMIFEDRKFADIGNTVKLQYSSGVYRIAEWADITNAHGVTGAGIVTGLKQAAQETTNEPRGLLMLAELSSKGSLAHGEYTQGTVEIAKTDKDFVIGFIAQRDMGGREEGYDWLIMTPGVGLDDKGDALGQQYRTVDEVVSTGSDIIIVGRGLFAKGRDPRVEGERNLCASLDVNTTEELLKLVDTLGPYICLLKTHIDIISDFSIDGTVKPLKELAKKHNFMIFEDRKFADIGNTVKLQYSSGVYRIAEWADITNAHGVTGAGIVTGLKQAAQETTNEPRGLLMLAELSSKGSLAHGEYTQGTVEIAKTDKDFVIGFIAQRDMGGREEGYDWLIMTPGVGLDDKGDALGQQYRTVDEVVSTGSDIIIVGRGLFAKGRDPRVEGER",
    "response#3": "LDLGIKTNTPRNFARRGIRCHVLPASTTFEQIAELNPHGVFLSNGPGDPATADHVVALTREVLGAGIPLFGICFGNQILGRALGLSTYKMVFGHRGINIPVVDHATGRVAVTAQNHGFALQGEAGQSFATPFGPAVVSHTCANDGVVEGVKLVDGRAFSVQYHPEAAAGPHDAEYLFDQFVQLCIGLDPDLAKFPAHLKGKPDAILTFCKEIIDATADTACAFKPQIAYFAALRAEDQLEAICSYLRERYPHIPIVLDAKRGDIGATAEQYAREAFERYGADAVTVNPYMGFDSVQPYLERPDRGVIVLCRTSNAGGSDLQFLNVDGKPLYQHVAQLVADKWNTNGQCALVVGATFPNELAQVRALVGDMPLLIPGIGAQGGDIKATVEAGRTANGTGMMINSSRAILYAKADETFAQAQLCIGLDPDLAKFPAHLKGKPDAILTFCKEIIDATADTACAFKPQIAYFAALRAEDQLEAICSYLRERYPHIPIVLDAKRGDIGATAEQYAREAFERYGADAVTVNPYMGFDSVQPYLERPDRGVIVLCRTSNAGGSDLQFLNVDGKPLYQHVAQLVADKWNTNGQCALVVGATFPNELAQVRALVGDMPLLIPGIGAQGGDIKATVEAGRTANGTGMMINSSRAILYAKADETFAQALERD"
  },
  {
    "reference": "XXKEIXWIFHDN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Has antibacterial activity against Gram-positive and Gram- negative bacteria including Salmonella, B.cereus, B.megaterium, L.casei, L.viridescens, M.flavus, C.glutamicum, C.crenatum, L.mesenteroides and E.faecalis.2. The designed protein localizes to the extracellular region. The designed protein sequence is ",
    "response#1": "GLLSGLKKVGKHVAKNVAVSLMDSLKCKISGDC",
    "response#2": "SLLGTVKDLLIGAGKSAAQSVLKGLSCKLSKDC",
    "response#3": "GLLSGLKKVGKHVAKNVAVSLMDSLKCKISGDC"
  },
  {
    "reference": "MQIYIVGGAVRDELLGRPNADCDYVVVGATPETMLAQGFRPVGKDFPVFLHPNTHDEYALARTERKTGHGYHGFIFHAAADVTLEEDLARRDLTINAMAKAADGTLVDPFNGQQDLASKTLRHVGPAFAEDPVRILRIARFAARFSDFSVAPETLALMRSMVACGEVDHLVAERVWQELAKGLMEQKPSRMFEVLRDCGALARLLPEVDKLFGVPQRADYHPEIDTGIHTMMVIDQSARRTFTLPVRFAALTHDLGKAETPADILPRHIGHEERSVRLTEQLCARLRVPNDCRDLALLTARYHGNIHRAADLKASTIVTLFEKTDALRRPERFRQLLDACLCDFTGRLGWENRPYDSPQYLLGALAAVASLNAGEIAVACADKTKIPERIHAARVHAIKQLLDNPGEQPEQQ",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have a low Km value for a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3   diphosphate.2. The protein requires Ni(2+) to activate a critical catalytic site.3. The designed protein should contain one or more HD domains that are essential for its function.4. The designed protein should have a binding site that is specific to the target ATP, CTP, Mg(2+) ligand.5. For general function, the protein need meet that These phosphohydrolase activities are probably involved in the repair of the tRNA 3'-CCA terminus degraded by intracellular RNases.; Also shows phosphatase, 2'-nucleotidase and 2',3'-cyclic phosphodiesterase activities; Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate; Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template6. The designed protein should be optimized for tRNA binding, magnesium ion binding, ATP binding, CCA tRNA nucleotidyltransferase activity, cyclic-nucleotide phosphodiesterase activity activity in order to improve RNA repair, tRNA 3'-terminal CCA addition. The designed protein sequence is ",
    "response#1": "MRIYTVGGAVRDRLLGLPVKDRDHVVVGSTPEAMVQLGYRPVGKDFPVFLHPVTHEEYALARTERKTGKGYKGFQVHADPEVTLEQDLARRDLTINAIAEDEHGNLIDPYHGVADVTMDRNGLIRDVDYEKAKKALDYIIPILEKTPHIYDVFVGGSYAKGTWLGRDIDIFVRFPKKYKGQNISIYIEQTLKEHNVPYIKLHGSRDYFQTFYEGLKIEIVPILKLDSPLEREFVTDISQFHVEWVKKNIKGLEGFVVADETMALCQRMVAAGEVDALVPERVWQELARGLMEPTPRRMIEVLRQAGALARLLPEVDSETMLEITFRVEETKEDIKNAKISRLTRKIVNYLEKNGIEVYAYTIDFDKNKSYLWVCCEKITIKTKHLGPPAWVNLDKFLEKHNKFFIREDGRLYTIINGNELTNRHI",
    "response#2": "MKIYLVGGAVRDQLLGYPHHEQDWVVVGATPQQMLDAGFKPVGKDFPVFLHPETQEEYALARQERKTAPGYTGFAFHADETVTLEQDLLRRDLTINAIAMDEEGTIIDPYNGKRDITTNVVREKAGRINDAKYFKIFNKLINTYGAESYLGGLSGYACEILTIHYKGFTNLLKGILKWKPKVFIDIEKHYSNIKEAINELGKDKTASPLILIDPVDKTRNVAASLSYKNFALIIANSYLYLNNEPVKHTKLPEEINDAKYFKIFNKLINTYGAESYLGGLSGYACEILTIHYKGFTNLLKGILKWKPKVFIDIEKHYSNIKEAINELGKDKTASPLILIDPVDKTRNVAASLSYKNFALIIANSYLYLNNTMLEITFRVEETKEDIKNAKISRLTRKIVNYLEKNGIEVYAYTIDFDKNKSYLWVCCEKITIKTKHLGPPAWVNLDKFLEKHNKFFIREDGRLYTIINSNK",
    "response#3": "MQIYKVGGAVRDRLLGKPVTDIDWVVVGATTEEMLAKGFRPVGADFPVFLHPKSGEEYALARTERKSGRGYGGFTFHASPEVTLEEDLIRRDLTINAMAEDDQQNLTDPYHGQRDLEARMVKDDKTYPYIYITDTGEHLLAVLRQCAEHGQPLVVRWACLLHDLGKGTTPAEEWPRHIAHERKGLKLIRAVNTRCKAPRDCQELSLLVGEYHSHCHRALELRPATLLELLQRFDVYRRPQRFEEFIAACEMDARGRLGLERNDAKYFKIFNKLINTYGAESYLGGLSGYACEILTIHYKGFTNLLKGILKWKPKVFIDIEKHYSNIKEAINELGKDKTASPLILIDPVDKTRNVAASLSYKNFALIIANSYLYLNNYKDAELVAQNNDAKYFKIFNKLINTYGAESYLGGLSGYACEILTIHYKGFTNLLKGILKWKPKVFIDIEKHYSNIKEAINELGKDKTASPLILIDPVDKTRNVAASLSYKNFALIIANSYLYLNNYTDAELVAQNNDAKYFKIFNKLINTYGAESYLGGLSGYACEILTIHYKGFTNLLKGILKWKPKVFIDIEKHYSNIKEAINELGKDKTASPLILIDPVDKTRNVAASLSYKNFALIIANSYLYLNNYKDAELVAQNNDAKYFKIFNKLINTYGAESYLGGLSGYACEILTIHYKGFTNLLKGILKWKPKVFIDIEKHYSNIKEAINELGKDKTASPLILIDPVDKTRNVAASLSYKNFALIIANSYLYLNN"
  },
  {
    "reference": "MRALLPHTQATWPLHDIAASRELEKAALQQRPAHQLIEQAGAAVARLAMALVPHGRSAVVLAGPGNNGGDALIAARWLAARGWRLQVYRFGPEPAAGSDAAHALRAAVDAGLEIRRGLPTVDGHPSPGSSAASGSGLIAGAPPAPIDLWIDGLLGLGAHRAPEGDIAAAIRLANRHPAPTLAIDLPSGLHADTGQTCGADTIRADHTLSLLTLKPGLVTGEGRAYAGRLWFDDLGVTPSPATAHWVGLDALTEWHQQHPRGATSHKGRQGDVVVLGGAPGMRGAAWLAATAALTAGAGRVYAALPDDDGQPWPARPELMHWPAQRWRQPQAWRDAVVVCGCGGDTGILPWLDAVLHEARHLVVDADGLNLVADDEALRKRLAARRSHGLQTMLTPHPLEAARLLGLAGAAAIQSDRLAAAKALAERFDCTVVLKGSGTVIASPGHLPDINSSGSPALATAGTGDVLAGWAGGLWAQAPDAEAHTVARTAVLWHGAAAQDHAGPLRAADLIEAMGALRRA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..2. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.3. The designed protein must be optimized for kinase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, metal ion binding.4. The AMP binding site should be stable and able to withstand changes in environmental conditions.5. The designed protein have YjeF N-terminal domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MKRIDQLAINKYKIPGVVLMETAAQRVAEKAREMLGERLQDKKTVVIAGKGNNGGDAFAAARHLHQWGCQVAVVSLVPAGEIKGDPEIYVRILEQLGIEINYLYREDDLPVLHKLIKESDLVIDGIFGTGIYGDITGFNAKAITAVNNGTAKVLSIDIPSGINGRTGQVCGVAIKADETVTFALPKPGLFQYPGAGYAGNISIADIGIPPEAVADTHKYKRGQVGVFSGGPNATGAARLSAMAAARSGAGAVTVLSPSDAMQVNAAHLTSIMLRAVDTVADAGEFINERQHTAFVLGPGFGVGEKARDFALRLLEQDEVYDFKRMGLFGGIVLDADGLTSFRDAPDVLFEAAHRTFAPSLVLTPHEGEFARLFPDIAKSKKQSKLAKARAAAARANAVIVYKGADSVIAAPDGRAAINANGAPWLATAGSGDVLAGIVAALLAQGMRAFQAACAAVWIHAEAGRRFGPGLIAEDLPLALVPVLRDLLAP",
    "response#2": "MKRIDQLAINKYKIPGVVLMETAAQRVAEKAREMLGERLQDKKTVVIAGKGNNGGDAFAAARHLHQWGCQVAVVSLVPAGEIKGDPEIYVRILEQLGIEINYLYREDDLPVLHKLIKESDLVIDGIFGTGIYGDITGFNAKAITAVNNGTAKVLSIDIPSGINGRTGQVCGVAIKADETVTFALPKPGLFQYPGAGYAGNISIADIGIPPEAVPRTSADTHKYKRGQVGVFSGGPNATGAARLSAMAAARSGAGAVTVLSPSDAMQVNAAHLTSIMLRAVDTVADAGEFINERQHTAFVLGPGFGVGEKARDFALRLLEQDEVYDFKRMGLFGGIVLDADGLTSFRDAPDVLFEAAHRTFAPSLVLTPHEGEFARLFPDIAKSKKQSKLAKARAAAARANAVIVYKGADSVIAAPDGRAAINANGAPWLATAGSGDVLAGIVAALLAQGMRAFQAACAAVWIHAEAGRERYLGKGDLELAPRRKRKGLNGKVAVIGGSEKYQGAPWLSALAAFRAGADLVFVYTPNRMDYPELIWRSLNPSDLLEGLYRDGVNVIVVGPGLHATSKEVVKVVLKYIEERKEARVVIDADGLKALAELDVKFSWRAVLTPHLGEASRLLGREVSDDLQSRIEAAGEIGNKYEACVILKGETDVVYCNGRTVLNDRGNAYMTVGGTGDVLSGVVGALLGRDEPWWAAKAATLAVTLAGEKCVKERGHSSPSCLIEEVPRVLSGRS",
    "response#3": "VMIGGDRGTAGAIRLAAEAAVRSGAGLVRVITHADNIGPLLTARPELMVDELSEHTLADALEWADVIVIGPGLGQGDWGKQALKKAENTAKPMLWDADALNLLAIRPDKRQNRIITPHPGEAARLLNVSIGEIEGDRVAAAQRLAKRYGGVVVLKGAGTIIASQQAELAIADVGNPGMASGGMGDVLAGIIGALTGQKLSLYDAACAGCVVHGAAADRVAGLKGPRGMLASDLFSADTHKYKRGQVGVFSGGPNATGAARLSAMAAARSGAGAVTVLSPSDAMQVNAAHLTSIMLRAVDTVADAGEFINERQHTAFVLGPGFGVGEKARDFALRLLEQDEVYDFKRMGLFGGIVLDADGLTSFRDAPDVLFEAAHRTFAPSLVLTPHEGEFARLFPDIAKSKKQSKLAKARAAAARANAVIVYKGADSVIAAPDGRAAINANGAPWLATAGSGDVLAGIVAALLAQGMRAFQAACAAVWIHAEAGRRFGPGLIAEDLPLALVPVLRQPPS"
  },
  {
    "reference": "MAEKAQNPMKELRISKLVLNISLGESGDRLTRAAKVLEQLSGQTPVFSKARYTIRRFGIRRNEKIACHVTVRGPKAEEILERGLKVKEYELKKRNFSATGNFGFGIQEHIDLGIKYDPSIGIYGMDFYVVMDRPGMRVARRKAQRGRVGYTHKINAEDTINWFKQKYDAVVLGK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain; The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules2. The protein must exhibit structural constituent of ribosome, rRNA binding. The designed protein sequence is ",
    "response#1": "MSLKDEVLKIMPVQKQTRAGQRTRFKAFVAIGDYNGHVGLGVKCSKEVATAIRGAIILAKLSIIPG",
    "response#2": "MSGQRTRFKAFVAIGDYNGHVGLGVKCSKEVATAV",
    "response#3": "MSIFKG"
  },
  {
    "reference": "MSAKGIRKNYQHWWRWGTMLLGMWMICSAAEELWVTVYYGVPVWKEATTTLFCASDAKAYDTEAHNVWATHACVPIDPNPQEVVLENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCSNLRNNTNTNSSTITNSSSEGMMEKGDMRNCSFNVTTTIKNKVKKEHALFYKLDITPIDNDTTSYTLINCNSSVVTQACPKISFEPIPIHYCTPAGFAILQCNDKKFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENFTNNAKTIIVQLNTSVEINCTRPNNNTRKGIHMGPGRTFYATGDIIGDIRQAHCNISKAKWENTLRQLAIKLREQFGNKTIAFKQASGGDPEIVMHSFTCGGEFFYCNTTNLFNGTWNGTAWNGTWNVSKTDKNITLSCRIKQIINRWQEVGKAMYAPPIAGKISCSSNITGLLLTRDGGNETNGTLEIFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKARRRVVQRGKRAVGTIGAMFLGFLGAAGSTMGAASLTLTVQARLLSSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLRDQQLMGIWGCSGKLICTTNVPWNTSWSNKSLNDIWNNMTWMEWDREVNNYTNLIYTLLEESQNQQEKNEQELLELDKWASLWNWFDITKWLWYIKIFIMIVGGLVGLRIIFSVLSIVNRVRQGYSPLSFQTHLPSPRGPDRPGGIEEEGGERDRGGSGRSVNGFLTLIWVDLRSLRL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be involved in positive regulation of establishment of T cell polarity by localizing to virion membrane, host cell plasma membrane, plasma membrane and exhibiting structural molecule activity.2. The protein must exhibit the following characteristics: Transmembrane protein gp41: Acts as a class I viral fusion protein; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. The designed protein sequence is ",
    "response#1": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNKSYEDIWEKMTWLQWDKEVNNYTEIIYGLIEESQNQQERNEKDLLALGKWASLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSLINRVRQGYSPLSFQTY",
    "response#2": "MGAASMALTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARILAVERYLRDQQLLGLWGCSGKLICTTNVPWNSSWSNKSHNEIWGNMTWMEWEKEIDNYSDVIYRLIEESQNQQEKNEKDLLALDSWASLWNWFDISNWLWYIRIFIMIVGGLIGLRIVFTVLSIVNRVRKGYSPLSLQTR",
    "response#3": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGMWGCSGKLICTTAVPWNLSWSDRTEEEIWGNMTWMQWDKEISNYSDTIYELLIKSQNQQDKNEKDLLALDSWENLWNWFSITKWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTR"
  },
  {
    "reference": "MVQYRALVIAVILLLSTTVPEVHSKSIIDRERRDWLVIPDAAAAYIYEAVNKVSPRAGQFLLDVSQTTVVSGIRNFLINETARLTKLAEQLMEKIKNLCYTKVLGY",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Design the protein with a signal peptide incorporated.2. The designed protein should be involved in lipid metabolic process by localizing to very-low-density lipoprotein particle, chylomicron and exhibiting nutrient reservoir activity, enzyme inhibitor activity. The designed protein sequence is ",
    "response#1": "MENATEWMTDLSDIESKFSFRTSEEPEDDLCYIVPGQPETIKECNFNPDTKTFIVIHGWTVTGMFESWVPKLVTALYDREPTANVIVVDWLSRAQQHYPTSAAYTKLVGRDVAKFVNWLQAEIDYPWERLHLLGYSLGAHVAGIAGLLTKHKVNRITGMDPAGPSFEYADAQSTLSPDDALFVDVLHTNTRGSPDRSIGIQRPVGHIDIYPNGGTFQPGCDLQNTVLMVATSGLRNMDQIVKCSHERSIHLFIDSLVNQDQESMAYRCSSKDSFNKGMCLSCRKNRCNKVGYGVNKIRTRRSSKMYMKTRNVMPV",
    "response#2": "MENATEWMTDLSDIESKFSFRTSEEPEDDLCYIVPGQPETIKECNFNPDTKTFIVIHGWTVTGMFESWVPKLVTALYDREPTANVIVVDWLSRAQQHYPTSAAYTKLVGRDVAKFVNWLQAEIDYPWERLHLLGYSLGAHVAGIAGLLTKHKVNRITGMDPAGPSFEYADAQSTLSPDDALFVDVLHTNTRGSPDRSIGIQRPVGHIDIYPNGGTFQPGCDLQNTVLMVATSGLRNMDQIVKCSHERSIHLFIDSLVNQDQESMAYRCSSKDSFNKGMCLSCRKNRCNKVGYGVNKIRTRRSSKMYMKTRNVMPA",
    "response#3": "MENATEWMTDLSDIESKFSFRTSEEPEDDLCYIVPGQPETIKECNFNPDTKTFIVIHGWTVTGMFESWVPKLVTALYDREPTANVIVVDWLSRAQQHYPTSAAYTKLVGRDVAKFVNWLQAEIDYPWERLHLLGYSLGAHVAGIAGLLTKHKVNRITGMDPAGPSFEYADAQSTLSPDDALFVDVLHTNTRGSPDRSIGIQRPVGHIDIYPNGGTFQPGCDLQNTVLMVATSGLRNMDQIVKCSHERSIHLFIDSLVNQDQESMAYRCSSKDSFNKGMCLSCRKNRCNKVGYGVNKIRTRRSSKMYMKTRNVMPR"
  },
  {
    "reference": "MAANKGINGGIKNHFIAFLGEFVGTFLFLFFAYGGTQTANQTSQKNPSIVASPDINQLLYIALIFGFSLTVNVWIFFRVSGGLFNPAVTIALCLVGVVGPVRSIFIFIAQVVASIAAAAAVRGLLPGDTVLFSCALAPGTSIAQGLFLEMFFTIELVFTILMLAAEKTKVTFVAPVGIGLSLFVAELMGVAWTGGALNPARAFGAEVIGGFRGYHWIYWLGPLMGAVLAAGFYKVIKFLNYEQVNGEQDLSAEEKQLKDEKKARKKEERRRKQNFAGLFQTGRMHHHHHANTRNATVNDAGDGRPNSAPPYTEPPAQQQTWPHSASTIRENEDFSRFAEMGSQDGIVITREQPAEQYRLSGERYVQDANAQNRAKTP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Involved in conidiation..2. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "ANSVLFFAHLCMFFEENRSKPIDLCIAFLSLTQLMLLVTMGLIAADMFMSQGIWDSTTCRSIIYFHRLLRGFNLCAACLLHILWTFTLSPRSSCLTKFKHKSPHHISCAFFSLCVLYMLFSSHLFVLIIATSNLTSDHFMYVTQSCSILPMSYSRTTMFSLVMVTREAFLISLMALFSGYMVTLLWRHKKQVQHLHSTSLSSKSSPQQRATRTILLLMSFFVVLYILDIVIFQSRTKFKDGSMFYSLHIIVSHSYATISPFVY",
    "response#2": "ANSVLFFAHLCMFFEENRSKPIDLCIAFLSLTQLMLLVTMGLIAADMFMSQGIWDSTTCRSIIYFHRLLRGFNLCAACLLHILWTFTLSPRSSCLTKFKHKSPHHISCAFFSLCVLYMLFSSHLFVLIIATSNLTSDHFMYVTQSCSILPMSYSRTTMFSLVMVTREAFLISLMALFSGYMVTLLWRHKKQVQHLHSTSLSSKSSPQQRATRTILLLMSFFVVLYILDIVIFQSRTKFKDGSMFYSLHIIVSHSYATISPFVS",
    "response#3": "ANSVLFFAHLCMFFEENRSKPIDLCIAFLSLTQLMLLVTMGLIAADMFMSQGIWDSTTCRSIIYFHRLLRGFNLCAACLLHILWTFTLSPRSSCLTKFKHKSPHHISCAFFSLCVLYMLFSSHLFVLIIATSNLTSDHFMYVTQSCSILPMSYSRTTMFSLVMVTREAFLISLMALFSGYMVTLLWRHKKQVQHLHSTSLSSKSSPQQRATRTILLLMSFFVVLYILDIVIFQSRTKFKDGSMFYSLHIIVSHSYATISPFVS"
  },
  {
    "reference": "MAPYHIRKYQDSDHRSVVDLFRRGMEEHIPATFRHMLLLPRTLLLLLGVPLTLFLASGSWLLVLLSILTLFLSLWFLAKYTWEKHVMNCLHTDMADITRTYLSSHSSCFWVAESRGQTVGMVAARPVKDPLLQKKQLQLLHLSVSLQHRREGLGKAMVRTVLQFAQMQGFSEVVLSTSMLQYAALALYQGMGFQKTGETFYTYLSRLRKSPMINLKYSLTSREGDL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should contain a Helical membrane-spanning region.2. A protein that can perform N-acetyltransferase activity, histone H4 acetyltransferase activity in endoplasmic reticulum membrane, Golgi apparatus, endoplasmic reticulum-Golgi intermediate compartment membrane, perinuclear region of cytoplasm to support axis elongation, negative regulation of cell adhesion. The designed protein sequence is ",
    "response#1": "LGDRILLDAEKAGTLRFCGTTEFASGQWVGVELDEPDGKNDGSVGGIRYFICPPKQGIFAPVSKIS",
    "response#2": "AFDARLFMERLRGKRLMFVGDSLNRNQWESMVCLVRPALSPGKSYVTWWDGQRVVLHAWEYNATVEFYWAPFLVESNSDDPKAHSIRDRVIKPEAIAAHAGDWVGVDYLVFNTYIWWMNTVNMKVVRPTGKTWEEYDEVGRIEAYRRVLDTWATWVNDNVDPARTSVFFMSVSPLHISPEAWGNPGGVRCAKEDAPVQNWHGPLWLGTDWDMFRAARNASRAAGRVPVTFVDVTAMSELRKDGHTSVHTIRQGRVLTPEQQADPATYADCIHWCLPGVPDVWNLMLYAR",
    "response#3": "AFDARLFMERLRGKRLMFVGDSLNRNQWESMVCLVRPALSPGKSYVTWWDGQRVVLHAWEYNATVEFYWAPFLVESNSDDPKAHSIRDRVIKPEAIAAHAGDWVGVDYLVFNTYIWWMNTVNMKVVRPTGKTWEEYDEVGRIEAYRRVLDTWATWVNDNVDPARTSVFFMSVSPLHISPEAWGNPGGVRCAKEDAPVQNWHGPLWLGTDWDMFRAARNASRAAGRVPVTFVDVTAMSELRKDGHTSVHTIRQGRVLTPEQQADPATYADCIHWCLPGVPDVWNLMLYA"
  },
  {
    "reference": "MRALLEPLGLRLVSAAERSLPPVEENGSSLAENARHKAIEIATASGLAAIADDTGLEVDALGGAPGLRSARWAGPGCSPEDNNRKLLAELQGFEEPQRRARFRTVIVWALPPRDGTPARVLHEVEGVLEGVIVAQPRGTGGFGYDPLFRPLGSDRTLAEMRPEEKNAISHRGRALAALRAALAACGRS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein with ITP diphosphatase activity, ribonucleoside triphosphate phosphatase activity, nucleotide binding is needed to enhance nucleotide metabolic process in a specific context.2. The protein should have a high affinity for Mg(2+), substrate.3. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP4. The presence of Mg(2+) is necessary for the protein to function in the desired environment.5. The protein should be designed to have high stability and activity towards dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MNIVLATRNAKKLAELTAMLAPLDIEVLSLADFPGMPPVVEDAPDFAGNARKKAVAAADATGLVTIADDSGIEVDALGGAPGVLSARWSGVHGDDAANNAKLLHQLADVPDERRGAQFHCAAALVAPDGTERVVEGIWPGRILTEARGEDTFGYDPVFLPDGETRTCAELTAEAKNAVSHRGQAFRALAPLVASLR",
    "response#2": "MKLVFATNNQHKTDEIRKQLGDQFELLSLADIGIDVDIPETGNTLEENAKQKADYVHTHYGYDCFADDTGLEVEALNGAPGVYSARYAGEQRNAEDNMQLLLNNLADKENRTAQFKTVIALNLNGKQHLFEGICKGSIITEKRGTNGFGYDPIFQPDGYDITFAEMDLSEKNKISHRAKAVQKLVEFLNSK",
    "response#3": "MKLSILIATRNAKKLGELRNMLAGQDIEVLGLSAFPEAPEVVEDGATFAENALKKARTAAAATGLPAIADDSGLCVDVLGGAPGIFSARWAGTHGDDAANTALLLDVPDTERGAAFVSVCALVTPDGQERVTEGTLEGSLVREPRGDGGFGYDPILQPAGLERTSAELSPEEKNAISHRGKAFRALAPALRAALG"
  },
  {
    "reference": "MAAATEPGARAWPGGGSPRPGSPACSPVLGQEAARGPGRAGPERAGARAPGPAAAPGHGFRKVTLTKPTFCHLCSDFIWGLAGFLCDVCNFMSHEKCLKHVRTPCTSVAPSLVRVAVAHCFGPRGLYKRKFCGVCRKVLEAPALRCEVCELHLHPDCVPFACSDCRQCHQDGHQDHDTHHHHWREGNLPSGARCEVCRKTCGSSDVLAGVRCEWCGVQAHSVCSAALAPECGFGRLRSCKTQSFRIAEAAWAGRGALVPPRGQAERRKKTLKIFDGDDAVRRSQFRLITVPRLASAEEVLEAALRAHHIPEDPGHLELRPLPPSSQACDAWAGGKAGNAVISEEGRSPGSGEATPEAWVIRALPRAQEVLKIYPGWLKVGVAYVSVRVTPQSTARSVVLEVLPLLGRQAKGPESFQLVEVAMGCRHVQRTVLADEQPLLARLQDIRQMSVRQLSQTRFYVAESRKVAPHISLFIGGLPPGLAPEDYSSLLLEAVATEATMLSVSHVYSSQGAVVLDVACFAEAERLYAMLKDTAVRGRLLTALVLPNLLHSRLPPDSRPLLVFVNPKSGGLKGRDLLCSFRKLLNPHQVFDLTNGGPLPGLHVFSQVPCFRVLVCGGDGTVGWVLGALEETRHRLACPEPSVAILPLGTGNDLGRVLRWGAGYSGEDPFSVLLSVDEADAVLMDRWTILLDAHDAGSAENGTADAEPPKIVQMSNYCGIGIDAELSLDFHQAREEEPGKFTSRLHNKGVYVRVGLQKISHSRGLHKEI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must have at least one Phorbol-ester/DAG-type, DAGKc domain.2. The protein should be optimized for stability and solubility when expressed in endosome where it will be involved in regulation of cortisol biosynthetic process. The designed protein sequence is ",
    "response#1": "LTGLRWGAFRDVAATISKYWYLGPLKTNAAHWFSTLREWPLVREISVSYLAPSLRPLDLNPQKPPRPNLLSRIITRLQNRWSPPVEEPPKEEEPEQWQEEHLSTLELFIQTQNKNPME",
    "response#2": "PDCCPLLVFVNPKSGGLKGRDLLCSFRKLLNPHQVFELTNGGPLPGFHVFSQVPCFRVLVCGGDGTVGWVLAALEETRRHLACPEPSVAILPLGTGNDLGRVLRWGAGYSGEDPFSVLVSVDEADAVLMDRWTILLDH",
    "response#3": "LTNLQWGPLKDVRQTVPKYWYFGPFSSWIAFAVASLRGTLRAKVTLPIQFVNVCEGCARCESVDVQESTTQTAENPRAWIEAVVPRRRMEVSAVQHQPERRFTHVNEDCGGIHESTLQAVDFQVLTQNAAPDQTTTTHGLVLSTIPQLGFQDFRDLSGTRIDADNVKGTNDRQRVRL"
  },
  {
    "reference": "FYHSSLAPTHELGGCWPPTGICPLNPPEVPLLNTSVLLASAYHSLTEGNRKHVLQALFITIALGIYFTLLQASEYYEAPFTISDRVYGSTFFIATGFHGLHVIIGSTFLIICFLRLSTYHFTTANISIPLNILTIMSRRHNIIAIPHGNPNDPKFTLHFSQNNTNHPVSVRSLQGHTQIILTSNSIKHIWHRLPSQSHLLKESPTPKKTIYHYTNHITNIFNHNIYSHRTNFILHYIRGYTSSYTHYHHSMSRATPTSSSISPISKTWRLLHTANYSNTQPADRMHSISLSYAFPMRNNHNQFNLHQTDLKSLITYSSISHIELYRSHCFNNRPRPHIIYATLPSKLKSRARANIVITALYYLYILITTQCGKYTHHINNITPSFTRENALIALHVLSLLLLSLNPKIILGPLYCKYRTKKLVQLQVRVTNLFSSFTLVTLLILTIPIIVTNTDLYKSNKYSSYVKNTVSCAFITSLIPRIIFVHAGQDIFFKYLLLFLITMLILVTANNLFQLFTGTRTSCSWKISPIWTSPLNPLSNRRPYSCISLLHSSTIVVAGIFLLIRFYPLTENNELIQTITLCLGALTTLFTAICLIMVTIGINQPYLVSIIHSLNDEQDIRKIGGLFKALPFTTTAPIIGSLALTGMPFLTGFYSRDLIIETANTARIIFFALLGQPPFSPLTSINENNPLLINSIKRLLIGSVFARFIISSNISPTKVPLKTMPLYLKLTALTVTILGFVLALEINLNTQKLKFIYPSNTLKFSSLLG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must be engineered to have a high affinity for heme b in order to increase its catalytic activity.2. The protein must be able to cytochrome-c oxidase activity. The designed protein sequence is ",
    "response#1": "MVRWLYSTNAKDIGTLYLIFAVFAAMIGTAFSVLIRMELAAPGVQYLNGDHQLYNVIITAHAFVMIFFMVMPAMVGGFGNYLVPVMIGAPDMAFPRLNNISFWLLPPSLILLLASAFVEQGAGTGWTVKCKLF",
    "response#2": "MVRWLYSTNAKDIGTLYLIFAVFAAMIGTAFSVLIRMELAAPGVQYLNGDHQLYNVIITAHAFVMIFFMVMPAMVGGFGNYLVPVMIGAPDMAFPRLNNISFWLLPPSLILLLASAFVEQGAGTGWTVKCKLL",
    "response#3": "MVRWLYSTNAKDIGTLYLIFAVFAAMIGTAFSVLIRMELAAPGVQYLNGDHQLYNVIITAHAFVMIFFMVMPAMVGGFGNYLVPVMIGAPDMAFPRLNNISFWLLPPSLILLLASAFVEQGAGTGWTVKCKLN"
  },
  {
    "reference": "MENFNQIFTLLAENEGIGLNTDILETGIINIAALVGILIYAGRDFLGSFLEQRKTSIVQGVQDAEGRLEEANRRLSEAQKQLSQAHIVISEIKNETISAKKVLLESDAYQAKKDLTTRFSRALATFRSKERQIFLEVKEQIILLVLKRTVARAQQTFGPKERATALITETINKLEGDLL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0).2. The protein should have Helical domains to ensure proper insertion into the membrane.3. A protein that localizes to chloroplast thylakoid membrane, proton-transporting ATP synthase complex, coupling factor F(o) and can perform ATP binding, proton-transporting ATP synthase activity, rotational mechanism with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "MRLTTFL",
    "response#2": "MLQMGRQPEAYGRILPLFFIGAALALFSNYFFN",
    "response#3": "MR"
  },
  {
    "reference": "MAKRVFLIILDSLGCGNAPDASAFGDQGSNTLAAVLSASDKPFPNLSSMGLFDIDGNSDPRILEYLSKDPQAGRPAPIGAYGRLREESSGGKDSTIGHWEIAGVVSEDPQPTYPDGFPSYVVDKLKEISGRGVLCNLPYSGTQAIEDYGEEHFSSGDLILYTSADSVLQIAAHEEIVPLDELYRICREMRTFMTGKDAVGRIIARPFTGTPGSFTRTANRHDFAVEAPSATMMDHLKAKGFDVISVGKIYDLFAGRGFTETNPTKGNSEGIAKIREYLDKDFTGLLFSNLVDFDMLYGHRNNIEGYNEALHEFDDALGDILESLKEDDLLIISADHGCDPSTVSTDHSREQVPLLIYGKGYSTPRNLGSITGFSYISQVVVNALSGARFEKRFPERDLDPSDPGDVMTYVDLTNLKVTATEDDIKALIDRAIASKTMSVCIPPCYVRSAYDYARGRIPICTVIGFPNGYNTTSVKVTEAKDAVDNGACEIDMVINVAFVKAGKMKEVEDEVKAIAGAVHEKGAILKVIIEACLLTEEEKVALCGIVERCGAEYIKTSTGFSTGGATVEDVALMRANLSSSVRIKAAGGIRSPEAARAMIDAGATRIGASGL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must be optimized for phosphopentomutase activity, manganese ion binding. The designed protein sequence is ",
    "response#1": "FARELGNLPPNYCTPAYLAETAAAFAGKFPGAEAEILDEQQMEALGMGSLLSVARGSANRPRLIVLKWNGGGEARPYVLVGKGITFDTGGVNLKTQGGIEEMKYDMCGGANVIGTFVATVKAELPINLVVVVPAVENAIDGNAYRPSDVITSMSGKTIEVGNTDAEGRLILCDALTYAERFNPEALVDVATLTGACMVALGHQTAGLMSKHDDLANELLAAGEHVFDRAWRLPLWDEYQGLLDSTFADVYNIGGRWGGAITAGCFLSRFTENQRWAHLDIAGVASDEGKRGMATGRPVGLLF",
    "response#2": "AERGLIVCGPLDRPGFAEAVTELARALDFPILADPLSQLRAGTHDKTYVLDSYDAILREETAASKLVPDVVLRFGAMPVSKPLFLWLKRHRAIRQIVVDDGGWRDPTLSADSFVQSDEWILCRQLLEWAKPKENKSAWSAIWREMNAIARAALERHLPKEEWFEGNVFTELADLLPAGAALFA",
    "response#3": "FARELGNLPPNYCTPAYLAETAAAFAGKFPGAEAEILDEQQMEALGMGSLLSVARGSANRPRLIVLKWNGGGEARPYVLVGKGITFDTGGVNLKTQGGIEEMKYDMCGGANVIGTFVATVKAELPINLVVVVPAVENAIDGNAYRPSDVITSMSGKTIEVGNTDAEGRLILCDALTYAERFNPEALVDVATLTGACMVALGHQTAGLMSKHDDLANELLAAGEHVFDRAWRLPLWDEYQGLLDSTFADVYNIGGRWGGAITAGCFLSRFTENQRWAHLDIAGVASDEGKRGMATGRPVGLL"
  },
  {
    "reference": "MEGASRHGLATARATLDEATAIPPGAAVTSVDASRVADDLRAVADLLHREPTVRRALTDPGAPPAARTELAARLLGRQLDAVSLRIVQTAVSSRWSRPADLQFALLELSVEALLVEAERDGALEEVEDELFRFARILGQNPQLALALTDPAAPASVKNALLGRLLSGRAHPVTLRLAQQAAADRIHGDVERRLADFSRIAAARRGRVVAVVRTAVPLTDEVIARLGAAISRYFGRQIQLQVDLDPELLGGVVVKVGDEVVDGSVLRRLAAARRALLR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that It either transmits conformational changes from CF(0) to CF(1) or is implicated in proton conduction. The designed protein sequence is ",
    "response#1": "SWAS",
    "response#2": "LTINA",
    "response#3": "GQTTVL"
  },
  {
    "reference": "MKKILQLIKQRSITRKLLVSFLSILIIPVVILAIFAYQSASSSLDRQMMGSALENVQQLNEIINTSIGEKENSADYFSEWLTKEKYNAKSNASIAEKFSQYISINKDVESIYTSDTKGHFTRYPDLPMPSGYNPVERDWYKKAVANKGKVVITDPYKTASTNTMVVTIAQQTKDGSGVIAINMTIENLLKTTKKVNIGTQGYAFIMTKDKKVVAHPNEQSGTELKGDWLDKMLSADKGDFQYTMDGDKKKMAFDTNKLTGWKIGGTMYLDEIHEAAQPVLHLALIVLAAAIIIGIIVMTLIIRSITTPLKQLVGSSKRISEGDLTETIDIRSKDELGELGKSFNNMASSLRSLIHAIQDSVDNVAASSEELTASAAQTSKATEHITLAIEQFSNGNEKQNENIETAAEHIYQMNDGLTNMAQASEVITDSSVQSTEIASEGGKLVHQTVGQMNVIDKSVKEAEQVVRGLETKSKDITNILRVINGIADQTNLLALNAAIEAARAGEYGRGFSVVAEEVRKLAVQSADSAKEIEGLIIEIVKEINTSLGMFQSVNQEVQTGLDITDKTEMSFKRISEMTNQIAGELQNMSATVQQLSASSEEVSGASEHIASISKESSAHIQDIAASAEEQLASMEEISSSAETLSSMAEELRDMTKRFKIE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be expressed specifically in the membrane raft The designed protein sequence is ",
    "response#1": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPV",
    "response#2": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPT",
    "response#3": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPQ"
  },
  {
    "reference": "MRRSLSLLLGSLLLTVGLLVGAQPSWAYPFWAQQNYDSPREATGKIVCANCHLAKKLTQVEVPQSVMPDTVFKAVVKVPYDTDVQQVGADGNRTALNVGAVVMLPDGYTLAPQDRLSDELKEETEGIYYTQYSDDQPNILLVGPLPGDEHQELVFPILAPDPKTDSSISYGKTQIHVGGNRGRGQVYPTGEKSNNTVYTASVAGDITAITGGDAGASVVTIAGDDGNVEETIPAGPALVVAVGDSVDAGEALTDDPNVGGFGQMDAEVVLQNPVRLYGLLGFLAAVAIAQIMLVLKKKQIEKVQAAEGV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions..2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. The protein must be engineered to have a high affinity for heme in order to increase its catalytic activity.4. The ideal protein would be one that plays a critical role in photosynthesis through heme binding, iron ion binding within plasma membrane-derived thylakoid membrane.5. The heme binding site should be stable and able to withstand changes in environmental conditions.6. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "VYDWFEERLEIQAIADDITSKYVPPHVNIFYCIGGITFTCFIIQVATGFAMTFYYRPTVAEAFASVEYIMTEVNYGWLFRSMHRWSASMMVLMMILHVFRVYLTGGFKKPRELTWVTGVTLAVVTVSFGVTGYSLPWDQVGYWACKIVTGVPEAIPVVGSLLVELLRGSVSVGQATLTRFYSAHTFVLPVAAAVLMLTHFLMIRKQGISGPG",
    "response#2": "TSKYVPPHVNIFYCIGGITFTCFIIQVATGFAMTFYYRPTVAEAFASVEYIMTEVNYGWLFRSMHRWSASMMVLMMILHVFRVYLTGGFKKPRELTWVTGVTLAVVTVSFGVTGYSLPWDQVGYWACKIVTGVPEAIPVVGSLLVELLRGSVSVGQATLTRFYSAHTFVLPVAAAVLMLTHFLMIRKQR",
    "response#3": "VYDWFEERLEIQAIADDITSKYVPPHVNIFYCIGGITFTCFIIQVATGFAMTFYYRPTVAEAFASVEYIMTEVNYGWLFRSMHRWSASMMVLMMILHVFRVYLTGGFKKPRELTWVTGVTLAVVTVSFGVTGYSLPWDQVGYWACKIVTGVPEAIPVVGSLLVELLRGSVSVGQATLTRFYSAHTFVLPVAAAVLMLTHFLMIRKQGISGPH"
  },
  {
    "reference": "MSVNLKGRSLLTLLDFSPEEIRYLLDISKQVKMENRSKLRTERFKGMTLAMIFEKRSTRTRLAFETAFAEEGGHPIFLSPNDIHLGAKESLEDTARVLGRMVDAIMFRGYKQETVEKLAEYSGVPVYNGLTDEFHPTQALADLMTIEENFGRLKGVKVVFMGDTRNNVATSLMIACAKMGMNFVACGPEELKPRSDVFKRCQEIVKETDGSVSFTSNLEEALAGADVVYTDVWASMGEEDKEKERMALLKPYQVNERVMEMTGKSETIFMHCLPAVKGQEVTYEVIEGKQSRVWDEAENRKHTIKAVMIATLL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The engineered protein is expected to adopt a helix-helix-turn-strand-helix-strand-turn-turn-helix-strand-helix-strand-helix-strand-helix-based conformation.2. The carbamoyl phosphate binding site should be stable and able to withstand changes in environmental conditions.3. The designed protein should interact with enzymes and substrates within the L-arginine biosynthesis, L-arginine from L-ornithine and carbamoyl phosphate: step 1/3, Amino-acid biosynthesis pathway to enhance or regulate its activity.4. A protein that can perform ornithine carbamoyltransferase activity, amino acid binding in cytoplasm to support arginine biosynthetic process via ornithine, citrulline biosynthetic process. The designed protein sequence is ",
    "response#1": "KGLTMTYLGDGANNMANSYLLAGVTAGMHVRIAAPAEYQPDESVVRDALNRADETGGSVLVTTDPRLAASGADVLATDTWVSMGQEDEKASRAEIFKPYALDAAALALAAADAVVLHCLPAYRGYEIAAEVIDGPQSVVWDEAENRLHAQKALMAWQA",
    "response#2": "KGLTMTYLGDGANNMANSYLLAGVTAGMHVRIAAPAEYQPDESVVRDALNRADETGGSVLVTTDPRLAASGADVLATDTWVSMGQEDEKASRAEIFKPYALDAAALALAAADAVVLHCLPAYRGYEIAAEVIDGPQSVVWDEAENRLHAQKALMAWR",
    "response#3": "KGLTMTYLGDGANNMANSYLLAGVTAGMHVRIAAPAEYQPDESVVRDALNRADETGGSVLVTTDPRLAASGADVLATDTWVSMGQEDEKASRAEIFKPYALDAAALALAAADAVVLHCLPAYRGYEIAAEVIDGPQSVVWDEAENRLHAQKALMAWN"
  },
  {
    "reference": "MATIQQLIRKGRKKIKYNSKSPALGGCPQRRGICTKVTTMKPRKPNSSMKKVAKVRLSNGKEVNVYIPGEGHNLQEHSAVLIQGGGVNDLPGINYRIIRGTLDTAGVNGRIQSRSRYGAKKGAAKQAAAAKSKKK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that contributes to translation by functioning in small ribosomal subunit with tRNA binding, rRNA binding. The designed protein sequence is ",
    "response#1": "MSS",
    "response#2": "MNRKSKKKWMGPSHPWIKINLGKEQILIGKYGLRNKKEIWIAQTMIRNFRHQARSLLALPPAERNIREKQLIQKLYRMGILEKDNSTLDDILSLTEENYLG",
    "response#3": "MGGLERRLQTIVYKKGLARTIYQARQLF"
  },
  {
    "reference": "MGGKWSKSSIVGWPAVRDRMRRAEPAAEGVGPASKDLDRHGALTSSNTATNNADCAWLEAQEGADEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLDGLIYSKKRQDILDLWIYHTQGFFPDWQNYTPGPGARFPLTFGWLYKLVPVDPREVEEANKGENNCLLHPISQHGMEDEDREVLQWQFDSSLARRHMARELHPEYYKDC",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis.2. The protein can be targeted to host cell Golgi membrane, membrane, extracellular region and perform SH3 domain binding.3. The designed protein should contain at least one Dileucine internalization motif; necessary for CD4 internalization motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "LQVL",
    "response#2": "GFASLL",
    "response#3": "LSSG"
  },
  {
    "reference": "MTAQPEIHLMAGGQDWDEIVTAAGQATDERIVVNMGPQHPSTHGVLRLILEIEGETVTDVRCGIGYLHTGIEKNLEYRTWTQGVTFVTRMDYLSPFFNETAYCLGVEQLLGIADEIPERANVIRVLMMELNRISSHLVALATGGMELGAMTAMFLGFREREMILKVFEAITGLRMNHAYVRPGGLAQDLPDGAEQEVRDLLEILPGRLRDMENLLNENYIWKARTQGVGYLDLTGCMALGITGPVLRATGLAHDLRRAQPYCGYENYDFDVVTHQDCDSYGRYLIRVKEMHESIKIAQQCVDRLRPGPVMVDDKKIAWPADLASGPDGLGNSPKHIAKIMGTSMEGLIHHFKLVTEGVRVPAGQVYVAIESPRGELGVHMVSDGGTRPYRVHFRDPSFTNLQSVAAMCEGAMVADLIAAVASIDPVMGGVDR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must be able to NAD binding, NADH dehydrogenase (quinone) activity, quinone binding. The designed protein sequence is ",
    "response#1": "MNHLIIFAHPNSVRSFGRAIANRIEQISQENGVNVFFRDLYEMNFNPILSHEELQNANNGIIPEDIQQEHDFILQADLITLVYPLWWMGFPAILKGYLDRVLSHGFAYKTENGESVGLLKNKQMQQFITIGSNVDKYKEFGVDKSLNHCLINGLFNYCGIENVEFELFGDIHLIDDKARKAMIELAAQKTL",
    "response#2": "MNHLIIFAHPNSVRSFGRAIANRIEQISQENGVNVFFRDLYEMNFNPILSHEELQNANNGIIPEDIQQEHDFILQADLITLVYPLWWMGFPAILKGYLDRVLSHGFAYKTENGESVGLLKNKQMQQFITIGSNVDKYKEFGVDKSLNHCLINGLFNYCGIENVEFELFGDIHLIDDKARKAMIELAAQKTL",
    "response#3": "MNHLIIFAHPNSVRSFGRAIANRIEQISQENGVNVFFRDLYEMNFNPILSHEELQNANNGIIPEDIQQEHDFILQADLITLVYPLWWMGFPAILKGYLDRVLSHGFAYKTENGESVGLLKNKQMQQFITIGSNVDKYKEFGVDKSLNHCLINGLFNYCGIENVEFELFGDIHLIDDKARKAMIELAAQKTQ"
  },
  {
    "reference": "MDYTISEFGKVFLFLLFGVVFVIGGYVSSRMLRPHRPNDEKLTSYECGEEAVGSAWVQFNIRFYVVALIFIIFDVEVLFLFPWATVFKQLGGFALFEAVIFVTILTLGLVYAWVKGDLDWVRPTPNVPKMPEKRFDNISSGRSQVVKEESVS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone; NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain; Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.. The designed protein sequence is ",
    "response#1": "ITTPLIRDGGDPKGALVPTSWSHAMAVAAQGLAAARGRTGVLVGGRVTWEDAYAYAKFARITLGTNDIDFRARPHSAEEADFLAARIAGRHMAVSYADLESAPVVLLVGFEPEDESPIVFLRLRKAARRHRVPVYTIAPFATGGLHKMSGRLIKTVPGGEPAALDDLATGAVGDLLATPGAVIMVGERLATVPGGLSAAARLADTTGARLAWVPRRAGERGALEAGALPTLLPGGRPLADEVARAQVCAAWHIAELPAAAGRDADGILAAAADETLAALLVGGIEPADFADPDAVLAALDATGFVVSLELRHSAVTERADVVFPVAPTTQKAGAFVNWEGRYRTFEPAESCGQCTPCREC",
    "response#2": "ITTPLIRDGGDPKGALVPTSWSHAMAVAAQGLAAARGRTGVLVGGRVTWEDAYAYAKFARITLGTNDIDFRARPHSAEEADFLAARIAGRHMAVSYADLESAPVVLLVGFEPEDESPIVFLRLRKAARRHRVPVYTIAPFATGGLHKMSGRLIKTVPGGEPAALDDLATGAVGDLLATPGAVIMVGERLATVPGGLSAAARLADTTGARLAWVPRRAGERGALEAGALPTLLPGGRPLADEVARAQVCAAWHIAELPAAAGRDADGILAAAADETLAALLVGGIEPADFADPDAVLAALDATGFVVSLELRHSAVTERADVVFPVAPTTQKAGAFVNWEGRYRTFEPAE",
    "response#3": "ITTPLIRDGGDPKGALVPTSWSHAMAVAAQGLAAARGRTGVLVGGRVTWEDAYAYAKFARITLGTNDIDFRARPHSAEEADFLAARIAGRHMAVSYADLESAPVVLLVGFEPEDESPIVFLRLRKAARRHRVPVYTIAPFATGGLHKMSGRLIKTVPGGEPAALDDLATGAVGDLLATPGAVIMVGERLATVPGGLSAAARLADTTGARLAWVPRRAGERGALEAGALPTLLPGGRPLADEVARAQVCAAWHIAELPAAAGRDADGILAAAADETLAALLVGGIEPADFADPDAVLAALDATGFVVSLELRHSAVTERADVVFPVAPTTQKAGAFVNWEGRYRTFEPAR"
  },
  {
    "reference": "MLSGVQPTGSLHLGNYLGAIRNWVAGQAEYENYFCVVDLHAITVPHDPAELAANTYTVAALYLACGIDPAHATIFVQSHVSAHAELTWLLNCITPLNWLEDMIQFKEKAVKQGENVAAGLLDYPVLMAADILLYDADLVPVGEDQKQHLELTRDIAARVNYLFARNQPPILKLPEPLIPKAGARVMSLTDGTKKMSKSDPSELSRINLLDSPDEIRKKIKRCKTDPIRGLAFDDPDRPEANNLLSLYQVLTGKTKEAVAAECADMGWGQFKPLLTDAVIATLEPIQQRYNEIMADPSYLKDLLKKGQEQAATVANATLERVKLAFGFTLP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The ideal protein would be one that plays a critical role in tryptophanyl-tRNA aminoacylation through ATP binding within cytoplasm.2. The designed protein should contain at least one 'KMSKS' region motif to achieve its intended function.3. The designed protein should have a high specificity for L-tryptophan, ATP binding, with minimal off-target effects.4. For general function, the protein need meet that Catalyzes the attachment of tryptophan to tRNA(Trp). The designed protein sequence is ",
    "response#1": "MNKFERL",
    "response#2": "MTPPAR",
    "response#3": "MPLAALRF"
  },
  {
    "reference": "MSLSSPETVESKVNLLGLSQQKLEAFFEEMGEKRFRAAQVLKWIHQMGVDSFDEMTNISKALRAKLSEKAEITAPELIQQFDSADGTRKFLIRVTGGSAIETVFIPDGERGTLCVSSQVGCSLDCSFCSTGKQGFNRDLSAAEIIGQVWIAAKSFGQFGEDRPRKITNVVMMGMGEPLLNFDNVVDSMNLMMHDNCYGISKRRVTLSTSGVVPALDKLDGLTDACLAISLHAPNDELRNELVPINKKYPIAMLLDSAKRYIDNLPDNRRKITIEYTLIDQVNDRPEHARELAQLLRDIPVKINLIPFNPFPMSDYKRVSNNSLRRFQDIMTQEGYITTVRTTRGDDIDAACGQLAGSVNDRTRRSQRYQEQAVRIINNA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have metal ion binding, tRNA binding, 4 iron, 4 sulfur cluster binding to facilitate rRNA base methylation.2. The Proton acceptor of the protein must be engineered to enable effective catalysis of adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.3. The [4Fe-4S] cluster binding site should be stable and able to withstand changes in environmental conditions.4. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs; m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity. The designed protein sequence is ",
    "response#1": "SMPPKHFADYTKPERIEILKELGLPKFRDNQIARQYYGRFQADPKKMTDLPESVRERIAETLFPPLMEPVRETSADDGNTQKMLWRLHDGTLLESVLMEYPDRATLCISSQAGCGMACPFCATGQGGLDRNLSTGEIVDQVRAAAATMQAKGGRLSNIVFMGMGEPLANYNRVLSAIHQITQPSPEGFGISQRGITLSTVGLAPAIRRFAKEGLSCRLAVSLHTPDDELRDSLVPANNRWPVEEVLDAAKEYADASGRRVSIEYALIKDINDQPWRADLLGKKLHGALGAKVHVNLIPLNPTPGSKWDASPKRVQDEFQRRVIARGVTCTVRDTRGQEIAAACGQLAAEERSGEILQAAS",
    "response#2": "SMPPKHFADYTKPERIEILKELGLPKFRDNQIARQYYGRFQADPKKMTDLPESVRERIAETLFPPLMEPVRETSADDGNTQKMLWRLHDGTLLESVLMEYPDRATLCISSQAGCGMACPFCATGQGGLDRNLSTGEIVDQVRAAAATMQAKGGRLSNIVFMGMGEPLANYNRVLSAIHQITQPSPEGFGISQRGITLSTVGLAPAIRRFAKEGLSCRLAVSLHTPDDELRDSLVPANNRWPVEEVLDAAKEYADASGRRVSIEYALIKDINDQPWRADLLGKKLHGALGAKVHVNLIPLNPTPGSKWDASPKRVQDEFQRRVIARGVTCTVRDTRGQEIAAACGQLAAEERSGRLRKSAETAS",
    "response#3": "SMPPKHFADYTKPERIEILKELGLPKFRDNQIARQYYGRFQADPKKMTDLPESVRERIAETLFPPLMEPVRETSADDGNTQKMLWRLHDGTLLESVLMEYPDRATLCISSQAGCGMACPFCATGQGGLDRNLSTGEIVDQVRAAAATMQAKGGRLSNIVFMGMGEPLANYNRVLSAIHQITQPSPEGFGISQRGITLSTVGLAPAIRRFAKEGLSCRLAVSLHTPDDELRDSLVPANNRWPVEEVLDAAKEYADASGRRVSIEYALIKDINDQPWRADLLGKKLHGALGAKVHVNLIPLNPTPGSKWDASPKRVQDEFQRRVIARGVTCTVRDTRGQEIAAACGQLAAEERSGEAAAK"
  },
  {
    "reference": "MLKIYNTLKREKEEFKPIHPDHVGMYVCGVTVYDLCHFGHGRTFVSFDVIARYLRYLGYNLRYVRNITDVDDKIIKRSLENNETCDQLVDRMIIEMHKDFDALNILRPDVEPRATQHIPEIIAIVEKLLAKGHAYVAEDGDVMFNVESFQKYGALSRQNLEQLQAGARVEIKSVKRNPMDFVLWKMSKENEPSWDSPWGKGRPGWHIECSAMNSKELGNHFDIHGGGSDLMFPHHENEIAQSCCAHGDDYVNYWLHTGMLTINEEKMSKSLNNFFTIRDILNKYDCESVRYFFLTAQYRSLLDYSEENIGLARKALERLYTALRGCDWNVELVENDQYVTAFKESMDDDFNTPGALAVLFELAREVNKLKAENQAEANKLAARLKQLAGVLGLLEQDPETFLQGDANNDEVAEIEAFIKQRNEARASKNWAVADEARNKLTAMGIVLEDGANGTTWRRA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit ATP binding, zinc ion binding, cysteine-tRNA ligase activity.2. The designed protein should have a binding site that is specific to the target Zn(2+) ligand.3. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity.4. The designed protein should contain at least one 'HIGH' region motif to achieve its intended function.5. The desired protein should be able to perform ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-   cysteinyl-tRNA(Cys) The designed protein sequence is ",
    "response#1": "FEPIEPGKVKMYVCGPTVYNYIHIGNARPAINYDVVRRYFEYQGYEVIYVSNFTDVDDKLIKRSKELDESVETITDRYIKAFYEDVGALNVKKATSNPRVMNHV",
    "response#2": "FEPIEPGKVKMYVCGPTVYNYIHIGNARPAINYDVVRRYFEYQGYEVIYVSNFTDVDDKLIKRSKELDESVETITDRYIKAFYEDVGALNVKKATSNPRVMNHV",
    "response#3": "RIYNTATRQVEEFTTYTPGLARGYVCGITPYDHVHVGHGRVYVFFDMFRRYLESLGYEVRLVVNFTDIDDKIIAKAREEFGPEAYKRWREVPERYIAEFFEVSKRLFIKPAYAYPRVTENVDDMVKWISALVEKGFAYVAPDGSVYFEVGKVPNYGVLSRQRIEELIAGARVEPEPGKRNPLDFALWKSWSPGEPWWNSPWCPGRPGWHLECVVMSTKHLGVPFDFHGGGADLIFPHHENEIAIAKAYFGVDNFAKYWIHVGYLTVRGEKMSKSLGNVITLREVLSKYSGEALRLAYAMSHYRKPMEFT"
  },
  {
    "reference": "MQMSEPLSQKNALYTAMNRFLGAVNNMDQTVMVPSLLRDVPLDQEKEQQKLTNDPGSYLREAEADMYSYYSQLKSIRNNIEWGVIRSEDQRRKKDTSASEPVRTEEESDMDLEQLLQFHLKGLHGVLSQLTSQANNLTNRYKQEIGISGWGQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a specific function within the cytosol. The designed protein sequence is ",
    "response#1": "MIF",
    "response#2": "MTILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVIPVSPKVAGKTLLGQKGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQLGF",
    "response#3": "HTIALVGASDKPDRPSYRVMKYLLDQGYHVIPVSPKVAGKTLLGQKGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQLGVINEQAAVLARDAGLNVVMDRCPAIEK"
  },
  {
    "reference": "MTKHIFVTGGVVSSLGKGITAASLGRLLKSRGYKVMMQKADPYLNVDPGTMSPFQHGEVFVTEDGKETDLDLGHYERFIDENLTSSSNFTTGLIYQSLISRERAGDFLGGTVQVIPHVTDAIKAQFRRVEEQTDADVVITELGGTIGDIEGQSFVEAIRQFRKEKGHGNTLVIHVSLVPYIAAAHEVKTKPTQHSVKELRSMGIQPDFIVCRSDHEVDASIRAKIAHFCDVDEDCVFENSDCPSIYDVPMHLANQDFDLKVCQKLGLDPHKRDMSEWNRFTDYMHEANAKEDVTTIKVVGKYTQLPDAYLSVIESLHHSGVYYGRHVQIDLIDGEELTPDNVDEVLAGADGILVPGGFGLRGIEGKVLSANRARVNKVPYLGICLGMQVAVSEFARNVCGMEGATSTEFDAECKYPVIDLMKDQEGVTDKGGTMRLGAYPCKLAEGTLAREAYGEELVYERHRHRFEFNNAYRKQLTDAGLVLSGLSPDGHLVEMIELRESDHPWFVATQAHPEFKSRPTKPAPLFREFVRAAIAGHEGVGRGEVVPEHGVAE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein should be able to manipulate the activity of enzymes in the CTP from UDP: step 2/2 pathway, either by activating or inhibiting their function.2. The designed protein must have a Nucleophile; for glutamine hydrolysis that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "LPDAYLSVSEAVRAAGFAHRARIQLRWVPSDECVSPTGAAAALSGVDGVVIPGGFGVRGIEGKIGAARYAREQGIPILGLCLGLQCMTIEVARHLAGLSGANSREFDEQAAHPVIATMADQADIVAGRGDLGGTMRLGAYPAVLTPDSLVAKAYEATEVSERHRHRYEVNNAYRDRLTQAGLRISGTSPDGRLVEFIELDAGLHPFFVATQAHPELKSRPTRPHPLFAAFR",
    "response#2": "VTVAIVGKYVDLPDAYLSVSEAVRAAGFAHRARIQLRWVPSDECVSPTGAAAALSGVDGVVIPGGFGVRGIEGKIGAARYAREQGIPILGLCLGLQCMTIEVARHLAGLSGANSREFDEQAAHPVIATMADQADIVAGRGDLGGTMRLGAYPAVLTPDSLVAKAYEATEVSERHRHRYEVNNAYRDRLTQAGLRISGTSPDGRLVEFIELDAGLHPFFVATQAHPELKSRPTRPHPLFAAFR",
    "response#3": "LPDAYLSVSEAVRAAGFAHRARIQLRWVPSDECVSPTGAAAALSGVDGVVIPGGFGVRGIEGKIGAARYAREQGIPILGLCLGLQCMTIEVARHLAGLSGANSREFDEQAAHPVIATMADQADIVAGRGDLGGTMRLGAYPAVLTPDSLVAKAYEATEVSERHRHRYEVNNAYRDRLTQAGLRISGTSPDGRLVEFIELDAGLHPFFVATQAHPELKSRPTRPHPLFAAFR"
  },
  {
    "reference": "MLDTQLNSSLSYKLFRADAVRDNEQQAAQESAVTLFTLMERAGEAIFRQCETAFPQADHLLVLVGQGNNAGDGYIAAHCAQKAGRKVTVCAVEPERKLDGDAGRAQQRWLEAGNSVSRFDDELLAQCGVVIDALLGTGINTYIRNEFAQIIDTVNHSSVPVISIDVPSGLDANTGHSLGRCVQADVTVTLVGIKPGLTTGAGKQSCGRLVFEDLGVSDAFSKLVKPFASWLDIEQFTRLGPRDVHSHKGSYGRLLCVGGNKGTAGAIRLSGEAALRSGAGMVRTYVHEKSIIQVSAGRPELMVSSEDLEDALQWATCVVIGPGLGQDDWAQQTFAQVLKHCQSEDKPVVLDADALNLLSQQASAYTLEHSILTPHAGEAARLLGVSVDDVESDRFNYAKQCAQRYHATCVLKGAGTIIDNETSVAVCRHGNPGMATAGSGDVLSGILGALLAQGMSLDIASRYGVTLHAKAGDDLAELYGQRGMLASDLFEAVRALINQ",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The desired protein should be able to perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate2. The protein must have high specificity towards ADP-dependent NAD(P)H-hydrate dehydratase activity. The designed protein sequence is ",
    "response#1": "GPLISSGAARLAAVAGLRAGAGLVTVASPPNAVLTQASHFTAIMQRALGGADALADWLGDRRLSAFVLGPGFGDADKARAFTRQLADTGRPVVLDADGITAHAACRDALGAAFGGAVRLALTPHEGEFARLFPDLAKDPALSKVDRARAAAAMLNAVVVYKGADTVIAAPDGRSAINADAPAWLATAGSGDVLAGILGGLLAQAMPVFEAACAAVAMHGLAARRR",
    "response#2": "GPLISSGAARLAAVAGLRAGAGLVTVASPPNAVLTQASHFTAIMQRALGGADALADWLGDRRLSAFVLGPGFGDADKARAFTRQLADTGRPVVLDADGITAHAACRDALGAAFGGAVRLALTPHEGEFARLFPDLAKDPALSKVDRARAAAAMLNAVVVYKGADTVIAAPDGRSAINADAPAWLATAGSGDVLAGILGGLLAQAMPVFEAACAAVAMHGLAARRR",
    "response#3": "MDQHKGQGGHTVVMGGSIPYLGAATMAGLGAVASGAALVTLSVHSSLAKEARMGYPGLMVASGNPEAPGWPRGKVASLVIGPGWGDEGLAYWFAVVMGDGPWCGLPTVFDADALNILAHDPGLRACDLPLNPSILTPHTGEAARLLAMSAEQVLENRVAAARVLAEKHRAVVVLKGPSTLIAAPDGRLAVNPTGHPLLAQGGTGDVLAGIIGGLQAQGMASFEAACCGTFLHGWAAERLAEHQGESGLRPELLN"
  },
  {
    "reference": "MPGQQRRGGGSGGSDRRERRDRSGGGPAQEKNAYVERVVAINRVAKVVKGGRRFSFTALVVVGDADGTVGVGYGKAKEVPAAIAKGVEEAKKHFFKVPRIGSTIPHPVQGEEAAGVVLLKPASPGTGVIAGGPVRAVLECAGVHDVLSKSLGSSNPINIVHATVAALRGLMRPEEIAARRGLPLEDVAPPAMLRARAAGAGV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body..2. The designed protein should be expressed specifically in the small ribosomal subunit The designed protein sequence is ",
    "response#1": "SLEERVVAVNRVAKVVKGGRRLRFSALVVVGDKNGHVGFGTGKAQEVPEAIRKAIEDAKKNLIEVG",
    "response#2": "ME",
    "response#3": "GLKEKLVMVNRVCKVTKGRRYFSFTALVIKGDENGVVGYGFGKAKEAQDAIYKAGEKAKKKLLKIL"
  },
  {
    "reference": "MGVPKKRTSSMRRDRRRAANFKLKPVNVVKCPKCKEPVLPHRACPSCGTYKGEQITEAS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have structural constituent of ribosome and be active in large ribosomal subunit. The designed protein sequence is ",
    "response#1": "LREYKVVGRLLPSAKNPTPPLYRMRIFAPNHVVAKSRFWYFVSQLRKMKKANGETVYCGLVHEQSPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTSGAVTQCYRDMGARHRARAHAIQIMKVS",
    "response#2": "LREYKVVGRLLPSAKNPTPPLYRMRIFAPNHVVAKSRFWYFVSQLRKMKKANGETVYCGLVHEQSPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTSGAVTQCYRDMGARHRARAHAIQIMKVL",
    "response#3": "LREYKVVGRLLPSAKNPTPPLYRMRIFAPNHVVAKSRFWYFVSQLRKMKKANGETVYCGLVHEQSPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTSGAVTQCYRDMGARHRARAHAIQIMKVI"
  },
  {
    "reference": "MWCLERLRLGPECLRRSGDWLLPGRARGAKSRTTAACANVLTPDRIPEFCIPPRLMPRLALAALRNSWVEEAGMDEGAGRTDWDPRSQAALSLPHLPRVRTAYGFCALLESPHTRRKESLLLGGPPAPRPRAHTYGGGGGPDALLGTLRVPRAPGPATPAAPGCPRPPQDALARRPRGCRLLRVPDGLLSRALRAGRSRRLTRVRSVSSGNEDKERRAGSQSPARAPSTSPPSSRVPFPERLEAEGTVALGRAGDALRLAAEYCPGTGRLRLRLLRAESPAGGAPGPRAVSCRLSLVLRPPGTALRQCSTVVGRSRKASFDQDFCFDGLSEDEVRRLAVRVKARDEGRGRERGRLLGQGELSLGALLLL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be designed to localize specifically to nucleolus in order to perform its role in regulation of cell adhesion, positive regulation of acute inflammatory response, regulation of vascular permeability involved in acute inflammatory response more effectively.2. For general function, the protein need meet that May be involved in inflammatory process The designed protein sequence is ",
    "response#1": "SCTACIRVDKTCSYCTDEIFKESRCDLRENLMSYGCSEANIMSVRGEMQVQQNFSIDTSLKKTQVAPQRMSMRLRAGEEVSFDMDVFQPLESPVDLYILMDFSYSMSDDLSNLKKMGQNLAEFLQSLTSNYTIGFGKFVDKVSSPQTDMRPAKLQEPWREADPPFSFKNVIRLTSNINHFSQELMKERISGNLDAPEGGFDAILQAAVCKENIGWRTDSTHLLVFSTESAFHYEADGTNVLSGILPRNDEACHLNQAGTYTYDTWQDYPSVPTLVRLLSRHNIIPIFAVTNHSYSYYEKLHKYFPISEIGVLQEDSSNILELIRVAFERIRSKLDIRADLIPKSMKTDFTSLKYEKTESGSFQVTRGEVGRFQVHVKAQEYVGGEHVCNLPEEDQQGEIHVKPSSLSDSLKIEASVICDIL",
    "response#2": "TAVVLGTRGKGKTVFLRKAMLDWASGVLLQNRFQYVFFFSVFSLNNTTELSLAELISSKLPECSETLDDILSNPKRILFVLDGFDYLKFDLELRTNLCNDWRKRLPTQNVLSSLLQKIMLPECSLLLELGESSCSKIIPLLQNPREIIMSGLSEQSIYFYCVSFN",
    "response#3": "ATAVVLGTRGKGKTVFLRKAMLDWASGVLLQNRFQYVFFFSVFSLNNTTELSLAELISSKLPECSETLDDILSNPKRILFVLDGFDYLKFDLELRTNLCNDWRKRLPTQNVLSSLLQKIMLPECSLLS"
  },
  {
    "reference": "MASEPAATVADLDFDNEQYDSAEDEDFQLDPDQEDADLTGSDSDDEATEPAAKRRKTGPTKAATEDQELDSGDEAMIQKVKARKEGKQKGKKSQGTASDEDEDDVDFDDDDEGGPGGFVRTRAMRMRMQEERKPLAKIDGATVDVDAIWAQMNAPDSSAGILPSQTQAKDATPAKDENKNADTAEEQVLSPDSKPQYKEEMVKIKRTYKFAGEVITEEKIVPKDSAEAKLFLAKGESVETVTDADVEHAANAKDALKLRRPLRKISRFDPNPTGTIKKSWEKQPTAGITGQTENISGPKINTVEKSRLDWAAYVDQAGIKDELKEHSKAKEGFLGRMDFLNRVVAKREEERRNARLKGL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein's functionality will benefit from Polar residues, Basic and acidic residues, Acidic residues compositional bias in its structure.2. The protein must exhibit the following characteristics: Component of the SWR1 complex which mediates the ATP- dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling; Involved in chromosome stability ..3. The protein should be optimized for stability and solubility when expressed in nucleus where it will be involved in chromatin organization.4. The protein contains novel BCNT-C domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "VLFHGPPGTGKTLMARALAASCSSDERKITFFMRKGADILSKWVGEAERQLRLLFEEAKKHQPSIIFFDEIDGLAPVRSSKQEQIHASIVSTLLALMDGMDNRGQVIVIGATNRPDAVDPALRRPGRFDREFYFPS",
    "response#2": "KVPRNKSHHDHLLESVTSSYSQMRQYAKLKQQNSRKVARMIAQHWERQLGTSEREKKAEERRLRALAS",
    "response#3": "VLFHGPPGTGKTLMARALAASCSSDERKITFFMRKGADILSKWVGEAERQLRLLFEEAKKHQPSIIFFDEIDGLAPVRSSKQEQIHASIVSTLLALMDGMDNRGQVIVIGATNRPDAVDPALRRPGRFDREFYFPS"
  },
  {
    "reference": "MINLAFITGNKHKVMEASILLKEYNIEVTQVSSEKVEIQSERLEEIVRYALESVRLTNRHILIEDAGLFIDSLNGFPGPYSSFAYKTIGVQGVLKLMEGMENRGARFVSVAGIKTLQNEVAIFRGEVEGVITHKPRGTKGFGFDPIFVPKGSSKTFAEMEVKEKNVFSHRAKALRRLAEWILGRRR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. We are looking for a protein with broad substrate specificity for dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "GLDMLKAGADLSKKTIDELIALDAKEFSMGPFKVEIAQANTVDTAEVLLRQAELETRIEALIAEKDLNLYMLLVTDILTNNSVALVLGPEASLVERAFGLEATNHLLYLEGVVSRKKQVVPVLTNTL",
    "response#2": "GLDMLKAGADLSKKTIDELIALDAKEFSMGPFKVEIAQANTVDTAEVLLRQAELETRIEALIAEKDLNLYMLLVTDILTNNSVALVLGPEASLVERAFGLEATNHLLYLEGVVSRKKQVVPVLTNTL",
    "response#3": "GLDMLKAGADLSKKTIDELIALDAKEFSMGPFKVEIAQANTVDTAEVLLRQAELETRIEALIAEKDLNLYMLLVTDILTNNSVALVLGPEASLVERAFGLEATNHLLYLEGVVSRKKQVVPVLTNTL"
  },
  {
    "reference": "MMILAWCIAWVLDFIIGDPQHWPHPVRWIGRLITFVQRIVRRYCPGDKALRIGGGVMWVVVVGATWGVAWGVLALAQRIHPWFGWSVEVWMIFTTLAGRSLARAAQEVERPLRENDLAESRIKLSWIVGRDTSQLQPAQINRAVVETVAENTVDGIIAPLFFLFLGGAPLAMAYKAVNTLDSMVGYKHEKYRAIGMVSARMDDVANYLPARLSWLLLGIAAGLCRLSGWRALRIGWRDRYNHSSPNCAWSEACVAGALGIQLGGPNNYFGERVDKPWIGDAQRDISVDDISRTIRLMWVASTLALALFIAARCGLSGLA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that However, the true cosubstrate could be (R)-1-amino-2-propanol O-2-phosphate, leading to cobinamide phosphate.; Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group2. The designed protein should be optimized for threonine-phosphate decarboxylase activity activity in order to improve cobalamin biosynthetic process.3. The protein should contain a Helical membrane-spanning region.4. We require a protein that can specifically bind to the metabolites produced in the adenosylcobalamin biosynthesis, Cofactor biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MLSTVL",
    "response#2": "LCGGR",
    "response#3": "LDITQL"
  },
  {
    "reference": "MLHIKDLIWTLYFIGTAVALEVNIVPDQGEISLGESKFFLCQVSGEATDISWYSPTGEKLVTQQQISVVRSDDYTSTLTIYNASSQDAGIYKCVASNEAEGESEGTVNLKIYQKLTFKNAPTPQEFKEGEDAVIICDVSSSIPSIITWRHKGKDVIFKKDVRFVVLANNYLQIRGIKKTDEGTYRCEGRILARGEINYKDIQVIVNVPPTIQARQLRVNATANMAESVVLSCDADGFPDPEISWLKKGEPIEDGEEKISFNEDQSEMTIHHVEKDDEAEYSCIANNQAGEAEATILLKVYAKPKITYVENKTAVELDEITLTCEASGDPIPSITWRTAVRNISSEATTLDGHIVVKEHIRMSALTLKDIQYTDAGEYFCIASNPIGVDMQAMYFEVQYAPKIRGPVVVYTWEGNPVNITCEVFAHPRAAVTWFRDGQLLPSSNFSNIKIYSGPTSSSLEVNPDSENDFGNYNCTAINTIGHEFSEFILVQADTPSSPAIRKVEPYSSTVMIVFDEPDSTGGVPILKYKAEWRVIGHEKWHTKYYDAKEVNAESIITVMGLKPETSYMVKLSAMNGKGLGDSTPSQEFTTQPVREPSAPKLVGHLSEDGNSIKVDILKQDDGGSPIRHYLVNYRALNALEWKPEMRVPSNSHHVMLKALEWNVDYEVIVVAENQQGKSKPALLSFRTTAKPTATTATASAGTGLGTGAIVGILIVIFVLLLVVVDVTCFFLNKCGLLMCIAVNFCGKAGPGAKGKDIEEGKAAFSKDES",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that This protein is a cell adhesion molecule involved in neuron- neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.2. The protein must contain a signal peptide for proper functionality.3. The designed protein should contain Cytoplasmic, Extracellular topological domains.4. The designed protein should have a binding site that is specific to the target heparin ligand.5. The protein contains novel Fibronectin type-III domains that confer a unique function or activity.6. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.7. The designed protein localizes to the plasma membrane. The designed protein sequence is ",
    "response#1": "FKSMQGDGVLFHGEGQRGDHITLELQKGRLALYLNLDDSKARLSSTVPLVILGSLLDDQHWHSVLLERVGKQANFTVDMNTQHFRTKGDTDSLDIDYELSFGGIPVPSKPGTFLKKNFHGCIENLYYNC",
    "response#2": "PITFINSRSSYLMLPGTPQIDGLSVSFQFRTWNEDGLLLSTELSEGSGTLLLILEGGTLRLLIKKVARHGTEILTGSGLNDGLWHSVSINARRNRVTLTLDNDAASPAPDTSRIQIYSGKSYYFGGCPDNLTDSQCLNPIKAFQGCMRLIFIDNQPKDLISVQQGSLGSFSDLHIDLC",
    "response#3": "FRTWNEDGLLLSTELSEGSGTLLLILEGGTLRLLIKKVARHGTEILTGSGLNDGLWHSVSINARRNRVTLTLDNDAASPAPDTSRIQIYSGKSYYFGGCPDNLTDSQCLNPIKAFQGCMRLIFIDNCPGFLKQPANIYAHESMDIVFECEVTGKPTPTVKWVKNGDVVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITAVQRADAGYYICQALTVAGSILAKAQLEVGGLG"
  },
  {
    "reference": "MRRLKENRNIAIIGFSATGKTVVAQKVAERLNWTFIDTDEEIVKLSGKTIPEIFKQDGEEKFRWLESQVLKQACHKEQMVIATGGGAIIDPKNQNLLLETSVVVCLEAKPETIYQRLLHDTLYSASPVVRPLLAGDNPLERIKQLKTKRQSYYAIADWTVHTDNLTVDEVSQEITKGWQYANRHRDKQSVEADLACRVQTPTAEYPVFVGWGMLDKLGEKMKQTVLSGTANIISDEIVFSIYGAKVRKALEKAGFAVNSCVVPAGEASKNINQATEIYDYLIEQRVERNDVIVALGGGMIGDLAGFVAATFLRGLPWLQVPTSLIAMTDASIGGKVAVDHPRGKNLIGAFYHPCLVLADVKTLTTLPHRELTSGWAEVIKHGLILDAEFLKLLEDKARDLVKLKPDITTSVIAGSAAIKCRVVSEDERETGKRTILNYGHTVAHGLEAATNYGCFLHGEAVAIGMMAAAKLSHRLKLLSEKAVERHEAILQKFGLPIDCSGVAVNDVLAAMELDKKVRGKAIRWVLLEDIGQVVIRSDVTEKDILAVLREVIRP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should interact with enzymes and substrates within the chorismate biosynthesis pathway to enhance or regulate its activity.2. The designed protein must be optimized for 3-dehydroquinate synthase activity. The designed protein sequence is ",
    "response#1": "TPGSKSISNRTLVLAALGKGTCRLRNLNLLHSDDTQVMMAALGELKGAKFSWEDNGDTLVVEGGEGSLSVPPKGKELYLGNAGTAARFLATVCTLVQKSPQDDADATIITGNARMKQRPIGPLVTALRANGSHITCLETDGCLPLAIKPEGLKGGVIQLAASVSSQYVSSILLCAPYAVEPVTLELTGGQVISQPYIDMTIAMMKTFGVDVVRRSDPTTGKFLDVYDIPKGVYVNPPTYNIESDASSATYPLAIAAITGTSCTIDNIGTSSLQGDAKFAKEVLEKMGATVTQTETKTTVQGPPIGSLKAIEEVDMEIMTDAFLTATAVAAVATGKTRILGIANQRVKECNRV",
    "response#2": "GMAEVVKTAAIWDEREFLSLEARSAEIFAAIQTPSQNFAGRTKNTRSAAQDLLLSVIVGSISVKAHIVTIDERETGLRNLVNFGHSIGHAIEAVLTPTILHGECISVGMILEAELSRQMGILSQVAVGRLSRCLKAYNLPITLSDPRIANLPAARLLTVDRLLDIMAIDKKA",
    "response#3": "GMAEVVKTAAIWDEREFLSLEARSAEIFAAIQTPSQNFAGRTKNTRSAAQDLLLSVIVGSISVKAHIVTIDERETGLRNLVNFGHSIGHAIEAVLTPTILHGECISVGMILEAELSRQMGILSQVAVGRLSRCLKAYNLPITLSDPRIANLPAARLLTVDRLLDIMAIDKKR"
  },
  {
    "reference": "MSTRTARSLGPDLVLAGTVAKRAEILVVGLTSGPDGPEIALSEGIVAEDVLAEILDSLIAVGATGKPEQLTRVPAPSALSVTSVLAVGLGSADKLDSEQIRKSAGAAARSLSGIDTVATTLSILDLGAAAEGFALGAYSFTEFKSSMTAPGPDSQPLARVELLVPSPRTKETKATLARSAAIAEAVATAREFVNTPPSHLYPAEFAARAKALGVEAGLTVQVLDEKALEKGGYGGIIGVGKGSSRQPRLVRLEYASKKRGARKVALVGKGITFDTGGISIKPAAGMENMTSDMGGAAAVISTVVLAAKLGLPVNVVAYVPMAENMPSATAQRPGDVLTQYGGITIEVVNTDAEGRLILADAMVRAGEDNPDYMIDTATLTGAQMVALGNRTPGVMGTDEFRDRVASISQSVGENGWAMPLPEELRGDLDSKVADMANVTPHRWGGMLVAAHYLKEFVPEGVQWAHIDVAGPAYNTSGPWGYTGKGGTGVPVRTMISVLEDIAVNG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. We are looking for a protein with broad substrate specificity for Release of an N-terminal amino acid, preferentially leucine,   but not glutamic or aspartic acids..2. The designed protein should be able to compete with endogenous proteins or inhibitors for Mn(2+) binding.3. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "LARELVAAPANVVTPPALAETARQLAQDYGLEVEILGREECQALGMGAFLGVAQASDLPPQFIHLTYKPKEGDPVAKLALVGKGLTFDSGGLNIKTDSRSIAMMKTDMGGAAAVLGAARALAALKPQVEVHFIVAATENMISGRALHPGDILTASNGKTIEVNNTDAEGRLTLADALVFAEKLGVDAIVDLATLTGACVIALGEEIAGLFTPDEKLAQELQQAANLSGEKIWRLPLEESYFEGLSSTVADMKNTGPRSGGSITAALFLKQFVEKTPWAHLDIAGPVWAEKDSGYTNKGATGYGVRTLL",
    "response#2": "LARELVNGPANSVTPITMAQTAETLASDYGLTLTILEQEDCEKLGMGAYLGVAKASDLPPKFIHLTYKPEGTPKRKLAIVGKSLTFDSGGLNIKVSGSGIETMKMDMGGGAATLGAAKAIAQLKPDVEVHFICAATENMISGKAMHPGDILTASNGKTIEVNNTDAEGRLTLADALVFAEQLEVDAIVDLATLTGACIIALGDDISGLWSPNEELATQLKTAAELAGEKFWQMPLEEKYFEGMKSPIADMKNTGPRAGGSITAALFLKQFIKETPWAHLDIAGPVWTDKEGGINNTGATGFPVRTLR",
    "response#3": "NLTRTLVNEPGNRLTPTLLGEQAKKMCAEQGLRCQVYSSEKLHELKMGSFWSVTQGSDEPPALIVMEYTPEGAAEGPVLGLVGKGITFDSGGLSLKPADSMEKMKYDMAGAAAMIGAMRAIALLKPRIKVISVICSAENMPSGKAQKPGDVQISMIGKSIEVLNTDAEGRLVLADGLAYAKQLGATHLIDAATLTGAVMVALGGVNAGVFCNDEEAWQHFEAALGQSGEKFWRLPLDEEYREMLRSPIADIKNVGGRYGGASTAAMFLKEFVGDTPWVHLDIAGTAWMDEAKPWMSSGPSGIAMAV"
  },
  {
    "reference": "MKLGRLLSVLNEKEFHGDEHYDIISIVSHSAAVQDKTLFVAIPGARVDGHDFLDIAYQAGARAFVTQRPFFKPDASTIIVPDARQALSLLAAEFYDQPTRAVTLIGITGTNGKTTITYLLESILQHAGHQVGVLGTVEYRCGSYREIAGQTTPEPMALQHVLRDMVQRGARYIVMEVSSQGLDQRRVDGCQFDIGVFTNLSPEHLDYHRTLENYYRSKERFFTQVLLNSAKKPVRAIINQDDPHGQKLAHSIPVPCLTYGISAGDIHATSVLRSSAGISADLNFPGGTMTVRSPLIGMFNLYNILAAASTALSLNLPLAAIGAGIEAVTRIPGRMERIENQRGILVLVDYAHTGDALENALQTVKDITARKVITVFGCGGDRDREKRPVMGAVAARYSDVVIITSDNPRSEPPEQIIADIEIGVRTAGVSKALDRYAAHHNSVKSYLVYPDRAVAIHEAIALARPGDSVLIAGKGHETTQHIGAQKFPFDDREAARRALHDSAAATG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a high affinity for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP.2. The presence of Mur ligase central, Mur ligase N-terminal catalytic domains is critical for the interaction of the designed protein with its intended targets.3. The designed protein should be involved in cell cycle, cell wall organization, regulation of cell shape, peptidoglycan biosynthetic process by localizing to cytoplasm and exhibiting magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, ATP binding, tetrahydrofolylpolyglutamate synthase activity.4. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.5. The protein's functional design necessitates the inclusion of a Meso-diaminopimelate recognition motif motif.6. Mg(2+) is required for the proper folding and stability of the protein.7. A protein should be able to manipulate the activity of enzymes in the peptidoglycan biosynthesis, Cell wall biogenesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "MKLNKIIKSIKVADDSRQIEAGDLFVAYPGDLADGRRYIADAVARGAVGVVWQPGGEFEWNPAWRVAQLAADDLRSLCGPLAVTGTNGKTTITQIIAQWLDSIGKRAAVMGTTGNGFLASLKEAKNTTGSAIEICKTLAQLNGLGADLTALEISSHGLVQHRIKALQFDAAVFSNLSRDHLDYHGDMQTYANAKLSLFTEHECKHKIINVDDAVGQQWLASVPDAIAVSMTEKPSTERYMYASDIHYSEQGIDIAFDSCWGAGRIQTSLIGEFNASNVLLAFGGLMALGLDDKAILDGMSAMQAVPGRMQLVAQRENGATVFVDYAHKPGAVIAALGSLRPHVMGRIVCVIGAGGDRDRGKRSLMGQAARDFADVVIVTDDNPRTEDPAAIRAAVMEGAGPEATEIPDRAEAILRGVDALLPGDALLICGKGHENYQEAKGVKLPFSDVEQATAALQKRLL",
    "response#2": "MKLSQILVAEDSRQVCPGDLFVAYAGDRADGRSYIADALERGAVAVLWQPGDDFAWDPAWTVPNQSMDNLRPMVEVVADDSRQIEAGDLFVAYPGDLADGRRYIADAVARGAVGVVWQPGGEFEWNPAWRVAQLAADDLRSLCGPLASALYAHPAQQLKLIGVVTGTNGKTSVSQWIARSHPKRCAVVGTLGAGFPDTLADTGFTTPEAACLARLLTGFCQQEAQVCALEASSIGIEEGRLNGAHVDTAVFTNFTRDHLDYHGSMDAYAAAKRKLFAWPRLRLAVLNLDDALGRQLARETTALKVLGYCIGDGHQEVLPAILRAENLVATPNGQTFALATPRGKVEVETRLLGRFNVSNLLAVAATLLALGESKQQLLFTASQLQPVCGRFEQFHSADNIVGIVDYAHTPDALENVLETITHLRHGNEKVITVVGCGGNRDAEKRPKMAAIACKYSNLVILTSDNPRYEDPLDILEQMRKGVPAQDYKKTIVIPDRREAIFKAGLEANPEDIILIAGKGHESYQEIKGQRLPFSDLAVAQLFLQTE",
    "response#3": "MKLSDLLSKLDILATNGDPAIEADSRKVTNGSLFVCIKGFTVDSHNFAKQAAAQGAAAIIAERPVDVDVPVVLEVQGVSTDTRRIAAGQLFVALRGEQFDAHDFLADAQRGGAVALMLSTPEKAPAGVPVVLVDDTRLALGRMAAALSDQPAAQLALAGVTGSNGKTTTKEMIASILKGVYGDAVLATQGNLNNDIGLPLTLLGLTPQHRVAVIEMGMNHPGEIAYLTALGAPTVAVVNNAQRAHLEGMGDLDEVAREKQTPVERVGGEGEPLVVVDYAHSPDALENALVTLREVAEARKGKLVAVFGCGGDRDRGKRPIMGELASRCADRVVLTSDNPRTEQPEAILQEIAVGVPAAEVIVDRAAAIRQTILTAEMADVLLLAGKQPAAPA"
  },
  {
    "reference": "MAEKVVAIVVAAGGGTRMGGPAPKQFLALGGRPILSWALAAAQAAACVDAIVVVCPAGWEDHTRRHCLAPFGLDKVRAVVAGGAQRQDSVAAGLEAALGLGAHWLLIHDGARPLARPELFAAVLEGARAHGAAIAGVPVVDTIKRSADGRLVQTTEDRRPLWRAQTPQGFRAELLAQALQAARAAGWSFTDEAGLFERLGREVRLIMGRADNIKITTPEDLALAQALLGPPAIRVGQGLDYHRLAPGRPLVLGGVRLEHELGLLGHSDADVLTHAVMDGLLAAAGLGDIGRLFPDHDPAHKDADSLVLLGRVVALLAEHGWRPAQIAVTLVAQGPKIAPHAPAMAQNLARVAGLSPGQVNVAATTTEAMGAIGRGEGMSALAVVTITPLEGAAEPAPSRRR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a high affinity for a divalent metal cation.2. The protein must exhibit the following characteristics: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D- erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C- methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF)..3. The protein should be designed to catalyze the following reaction 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-   erythritol 2,4-cyclic diphosphate + CMP.4. The protein must be engineered to have a cooperative binding mechanism with a divalent metal cation to ensure proper regulation.5. We require a protein that can specifically bind to the metabolites produced in the isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6 pathway. The designed protein sequence is ",
    "response#1": "RMGNGYDVHAFEDGDHVVLCGVKIPHDRKLKGHSDADVGMHAITDAIYGALAEGDIGRHFPPSDPQWKGAESRIFLAHAVDLAAKRGFAISNVDCTLICEEPKIGPHAQAMQDALAQIMGLPADRISVKATTSEKLGFTGRSEGIASVATCVLAEKGTPVPEKPVVGMGYDVHRLVADKPLWIGGIEIAHSHGLEGHSDADVGLHALTDAILGALADGDIGDHFPPSDPQWRGVASHRFLEFAANRVVEGGGQIDHVDLTIIAEAPKIGPHRSAMRARIAEILAIPLDRVSVKATTTERLGFTGRREGIAAQAVATVLDAEGSPVEPRVGVGTDVHRWAPEDAPRPLWLGGVHWPGERGVAATSDGDVAAHAACNALLSAAGLGDLGAVFGVDRPEMADASGTAMLAEVLRLLTEAGLAVGNVAVQVVAPRPKVGTRRAEMEAALSAALGGAPVSVSAATTDRLGFIGRGEGLVGIATALVAGLDVHRLEDGEELWLGGVLIPHTQGLSGHSDADVALHAITDALLGTIAAGDIGQHFPPSDPQWKGAASDQFLAHAATLVRDRGGQIGFIDLTLMCEAPKIGPHREAMRRRIAAILNIAEERVSVKATTTEKLGFTGRGEGIAAQAIATILDAEGKPVPRVGVGTDVHRWAPEDAPRPLWLGGVHWPGERGVAATSDGDVAAHAACNALLSAAGLGDLGAVFGVDRPEMADASGTAMLAEVLRLLTEAGLAVGNVAVQVVAPRPKVGTRRAEMEAALSAALGGAPVSVSAATTDRLGFIGRGEGLVGIATALVAVRD",
    "response#2": "RMGNGYDVHAFEDGDHVVLCGVKIPHDRKLKGHSDADVGMHAITDAIYGALAEGDIGRHFPPSDPQWKGAESRIFLAHAVDLAAKRGFAISNVDCTLICEEPKIGPHAQAMQDALAQIMGLPADRISVKATTSEKLGFTGRSEGIASVATCVVVGRADRLAAALRVGVGTDVHRWAPEDAPRPLWLGGVHWPGERGVAATSDGDVAAHAACNALLSAAGLGDLGAVFGVDRPEMADASGTAMLAEVLRLLTEAGLAVGNVAVQVVAPRPKVGTRRAEMEAALSAALGGAPVSVSAATTDRLGFIGRGEGLVGIATALPRVGVGTDVHRWAPEDAPRPLWLGGVHWPGERGVAATSDGDVAAHAACNALLSAAGLGDLGAVFGVDRPEMADASGTAMLAEVLRLLTEAGLAVGNVAVQVVAPRPKVGTRRAEMEAALSAALGGAPVSVSAATTDRLGFIGRGEGLVGIATALVRR",
    "response#3": "RMGNGYDVHAFEDGDHVVLCGVKIPHDRKLKGHSDADVGMHAITDAIYGALAEGDIGRHFPPSDPQWKGAESRIFLAHAVDLAAKRGFAISNVDCTLICEEPKIGPHAQAMQDALAQIMGLPADRISVKATTSEKLGFTGRSEGIASVATCVVEAPSAEVVEAVREALVS"
  },
  {
    "reference": "MSSLSVYHVSSPDIPNKVLTHFEDIAATLAEQGIHFARWEAAVKIQPGASEEDIIAAYQAQIDGLMTERGYVGVDVVSVSSDQPEQDGELDEYRHSQDEVRFFVTGRGLFNLHLGDYVYAVLCERNDLITVPAGTPHWFDRGERPHFVSIRLSGDLQGLVAEVTGDDIARRFPRLED",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Ni(2+) is required for the proper folding and stability of the protein.2. A protein with nickel cation binding, acireductone dioxygenase [iron(II)-requiring] activity functions is required to enable L-methionine salvage from S-adenosylmethionine, L-methionine salvage from methylthioadenosine.3. The protein design should consider the endogenous proteins and enzymes present in the Amino-acid biosynthesis, L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6 pathway to avoid interference with native metabolic processes.4. The protein must exhibit the following characteristics: Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.; Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4- methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway.5. The designed protein should be able to compete with endogenous proteins or inhibitors for Ni(2+), Fe(2+) binding. The designed protein sequence is ",
    "response#1": "MDYAPLTERELAAYGQTEDSLPADIRAEVLLTDGPVLLVDDEDRENEGDLIIAAEKATPELLAFMVRYTSGYICVALPGEDCDRLGLPPMYHTNQDRRGTAYTVSVDAAEGTSTGISAGDRATTLRVLADPASRPEDLTRPGHVFPLRARPGGVLRRAGQTEAAVDLARLAGLRPAGVLCEILNDDGTMARRPDLEVFAQEHGLVLISIADLIAYRRRTERLVRRV",
    "response#2": "MSIAAASRLARSLAGLGRAPSRALWRPSSASASATARLRPSPARWMSSSLRSSSSSTGGSGSGGSGKHVNWFDLFRTKPKVPEPEVPQPEGEKEGEDQPKAGPEEKPAVPPETPLDKLDALKAEAAAQDVQITPDGTCLSVGEAAPSPPADVKSKSRITLPLTPLRSSSSSTSSSTNADAGATGVTGDGEGSASPHLLAKLAGLTNVPLATLEPIDFKRLAKPPLVSSGEPNTLLRPILPPPAPEPAPTTTASASTTTTTAATTAEAPSLEIFRKYAGFEPPEGESAEERRKRIWQEKFKAKREGKDVPPPKLRRKDMPWTKPRDERWAAREAAEKAARDARLAQKAGGKK",
    "response#3": "MRCTIDFDSIDDALEDVRAGKTVVVVDDEDRENEGDLVIAAEKATPEVINFMATHGRGLICAPLTAERCDELGLELMVGRNTARHETPFTVSVDLLGAVSTGISASDRATTVRALVDPASRPEDLGRPGHIFPLRAKPGGVLRRAGHTEAAVDLARLAGLEPAGVLIEIMNEDGTMARLPQLVEVARRFDLKLISIRDLIAYRLRTESLIEREAVALPTRHGPFQVHAMRTEKLGHIAVPSHEHPNVHHNRLPTKSNRHGVTLTHDGVGVRYDPKPSTPAALRALPELSHADLVDLLDRLDHLVTHHDVLRDEDDVGVRLHAFGDDPMAVLVRRDEALSKRPDLAPIARERFDRIEASGADWLARIRDDRGVALALAPAPDPVAALLAGLDRVTTEPLRTREEAARHGPHHPYTRALREIVDALPADLTRDLPPEAVLALHW"
  },
  {
    "reference": "MARKKISIIGAGNVGATAAHWAASKELGDIVLLDIMEGIPQGKGLDLMEASPVEGFDCHIIGTNSYADTANSDVVVITAGIARKPGMSRDDLITTNTKIVADCAKKAAEQSPDSIIIIVSNPLDAMTYVAQKASGFPTNRVFGMSGILDAARFKTFIAMEMGCSVKDVSTILLGGHGDDMVPLPSYTFIGGIPIRQLLPEEKIQAMVDRARKGGAEIVAYLKTGSAYYAPSASVIQMVEAILKDQKRILPVAAYLNGEYGYEGIYTSVPVMLGANGVEKVFEIELTAEERQLFAKSVDSVKNLIAVTGM",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The designed protein should have L-malate dehydrogenase activity to facilitate tricarboxylic acid cycle, carboxylic acid metabolic process.3. The protein should have a high affinity for substrate, NAD(+). The designed protein sequence is ",
    "response#1": "TTLDTIRSEKFIVQAKNIEINRNDISVIGGHSGVTILPLLSQIPHVEFTEQELKDLTHRIQNAGTEVVEAKAGAGSATLSMAYAAMRFVVSMARALNGEVITECAYIEGDGKFARFFAQPVRLGKNGVEEILPLGTLSAFEQQALEAMLPTLQTDIDNGVKFI",
    "response#2": "LDSNRMAYYASQIIGIAPESIIPVVLGQHGENMYPVPEASFVYGKPLTEFLTQEQYNDIVKKTIQAGADITNLRGFSSNWGPAAGLALMVDSIKKNRRRVFEASVYLDGEYGVKDVFAEVPVVLGKNGVEKIIELNLTPEQRQKFMQSIEAVKKNLTQA",
    "response#3": "LDSNRMAYYASQIIGIAPESIIPVVLGQHGENMYPVPEASFVYGKPLTEFLTQEQYNDIVKKTIQAGADITNLRGFSSNWGPAAGLALMVDSIKKNRRRVFEASVYLDGEYGVKDVFAEVPVVLGKNGVEKIIELNLTPEQRQKFMQSIEAVKKNLTQY"
  },
  {
    "reference": "MRVRGTLRNYQQWWIWGVLGFWMIYNVGGNLWVTVYYGVPVWTEAKTTLFCASDAKTYETEVHNVWATHACVPTDPNPQEIELKNVTENFNMWKNEMVNQMHEDVISLWDQSLKPCVKLTPLCVTLKCRNINYTTTNGSDIQPYNSSEGKEIKNCSFNATTELKNKKQKVYALFYRLDLVPLGKEEDSSGNYRLINCNTSAITQACPKVTFDPIPIHYCTPAGYALLKCKDKKFNGIGPCSNVSTVQCTHGIKPVVSTQLLLNGSLAEEDIIVRSENLTDHFKTIIVHLNQSVEIVCTRPNNNTRKSIRIGPGQTFYATGGIIGDIRQAHCNISEKSWHETLYNVSKKLAEHFPNKTIQFTNSSGGDLEITMHSFNCRGEFFYCNTSGLFNSTYILNGTYMYRANGTDKNTTDGTISNPNSTITIPCRIKQIVNMWQEVGQAMYASPIAGNITCKSNITGLLLVRDGGGEKNDTEIFRPGGGDMRDNWRSELYKYKVVEIQPLGVAPTKAKRRVVEREKRAVGIGAVFLGILSVAGSTMGAASMALTVQARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLRDQQLLGIWGCSGKLICTTAVPWNSSWSNKTEDDIWKNMTWMQWDREISNYTDTIYRLLEASQYQQERNEKDLLALDSWENLWNWFDITKWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTHIPNPGGPDRLRGIEEEGGEQDKTRSVRLVSGFLALAWDD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design must feature the YXXL motif; contains endocytosis signal motif.2. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.3. Design the protein with a signal peptide incorporated.4. A protein that can perform structural molecule activity in host cell plasma membrane, plasma membrane, virion membrane, viral envelope, host cell endosome membrane to support positive regulation of receptor clustering, clathrin-dependent endocytosis of virus by host cell, positive regulation of establishment of T cell polarity, virion attachment to host cell, fusion of virus membrane with host endosome membrane.5. The presence of Retroviral envelope protein GP41-like, Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains is critical for the interaction of the designed protein with its intended targets.6. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWRDAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQEIHLENVTEDFNMWKNDMVDQMNEDIISLWDQSLKPCVKLTPLCVTLNCVSISNDTSKLWVTVYYGVPVWKEATTTLFCASDARAYDTEVHNVWATHACVPTDPNPQEVVLKNVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLHCTDLKNGTNGTSSTSNSEGTMEGGEIKNCSFNITTSIRDKVQKEYALFYKLDVVPIDNTSYRLISCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNDKKFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSNNFTDNAKTIIVQLNKSVEINCTRPNNNTRKSIPIGPGRAFYATGEIIGDIRQAHCNISRTKWNNTLSQIVKKLKEQFGNKTTIVFNQSSGGDPEIVMHTFNCGGEFFYCNTTKLFNSTWNGTERSIGTEENITLPCRIKQIVNMWQEVGKAMYAPPIRGQIRCSSNITGLLLTRDGGSNTNETETETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVLGAMQAQEHSAALWVTVYYGVPVWKEAKTTLFCASDAKAYDKEVHNIWATHACVPTDPNPQEIVMGNVTENFNMWKNDMVEQMHEDVISIWDQSLKPCVKLTPLCVTLNCTNATVTNSTKAGGTISPSTTVAYNDSIYVEIKNCSFNTTTEIRDRQQKAYALFYRSDIVQLKGNSNSSDYILINCNTSTITQARPKVTFDPIPVHYCAPAGYAILRCNNNTFNGTGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAKGDIIISSENLTDNAKTIIVHLNESVEIVCTRPGNNTRKSIRIGPGQTFYATGGIIGDIRKAYCNISRSNWNTTLEKVRKKLEEYFPNRTIGFAPSSGGDPEITTHSFNCRGEFFYCNTSGLFNSTLFNSTSNSTSNSTDIILPCRIKQIINMWQEVGRAMYAPPIAGNITCKSNITGLLLTRDGGKNISKTETFRPGGGNMKDNWRSELYKYKVVEIKPLGIAPTSAKRRVVEREK",
    "response#2": "LWVTVYYGVPVWKEATTTLFCASDARAYDTEVHNVWATHACVPTDPNPQEVVLKNVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLHCTDLKNGTNGTSSTSNSEGTMEGGEIKNCSFNITTSIRDKVQKEYALFYKLDVVPIDNTSYRLISCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNDKKFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSNNFTDNAKTIIVQLNKSVEINCTRPNNNTRKSIPIGPGRAFYATGEIIGDIRQAHCNISRTKWNNTLSQIVKKLKEQFGNKTTIVFNQSSGGDPEIVMHTFNCGGEFFYCNTTKLFNSTWNGTERSIGTEENITLPCRIKQIVNMWQEVGKAMYAPPIRGQIRCSSNITGLLLTRDGGSNTNETETETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR",
    "response#3": "LWVTVYYGVPVWRDAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQEIHLENVTEDFNMWKNDMVDQMNEDIISLWDQSLKPCVKLTPLCVTLNCVSISNDTSKTGWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLENVTEKFNMWKNNMVEQMQEDIISLWDQSLKPCVKLTPLCVTLNCTDMNNSTTNTSRNNWEKMEQGEVKNCSFNITSSMRDKVQKEYATFYKLDVVPIANNNNNTRYRLISCNTSVITQACPKISFEPIPIHYCTPAGFAILKCNDKSFNGTGLCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIKSSNFSDNAKTIIVQLNASVQINCTRPNNNTRKSIHMGPGKTFYAAGDIIGDIRQAHCNLSRAQWNKTLELVVGKLKEQFNKTIIFNQSSGGDPEIVMHSFNCGGEFFYCNTAELFNSTWDTTTGSNNTNDTEIITLPCRIKQIINMWQQVGKAMYAPPIKGQIRCSSNITGLLLTRDGGNNTDNETFRPGGGDMRDNWRSELYKYKVVKIEPLGLAPTKARRRVVQREKRAVGLGMLFLGVLSAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKSQEEIWENMTWMQWDREINNYTNTIYSLLEESQNQQEKNEQDLLALDKWQNLWSWFSITHWLWYIKIFIMIVGGLIGLRIIFTVLAIVNRVRQGYSPLSLQT"
  },
  {
    "reference": "MSKFSPPINRNMVELDRSFFHKEVPLLAAYFPNPKFLGQFVKSCQNDILYVQTVKHIISMDDSKAILLRDDVKSISDLNPETQLKINEFGIILKPYTLKLDYSFWKSEEILKSILPENLIDDVPSGFSQAGHLAHINLRDEYKPFGKLIGQVILDKNPSVLTVVDKVNTIANKFRTFPLELLAGEPNYIVEQSESGCKFKFDFSKVYWNSRLSTEHERIIGKFNSGDVVGDVFGGVGPFAIPASKKNVIVLANDLNPESYKYLQENIKINKVEPFIKPFNLDGREFIRKAPELLLQWHNSQNGIIEKKIIKKVSIDDNKTKKNFERKPIIETTKIPKFYHHFVMNLPDSALTFLDEFIGLYGSNPQLKTDPEFKLPIIHVHCFEKFENNENPTPEELHNRVYEKICKLIQFPLNKEKMEFHEVRMVSPTKPMFCVSFELPEEVAFKQSK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a fast turnover rate for guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-   methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine.2. A protein that contributes to mitochondrial tRNA methylation, tRNA N1-guanine methylation by functioning in nucleus, cytoplasm with tRNA methyltransferase activity, tRNA (guanine(37)-N(1))-methyltransferase activity, tRNA (guanine-N1-)-methyltransferase activity.3. For general function, the protein need meet that Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs; Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside; This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding. The designed protein sequence is ",
    "response#1": "MFSMVGHVAHLNLRSEWKDYDRLIGQVILDKNPRVKTVVNKVDSIDTKFRTFKMDVLAGEDNTEVEQHESGCRFQFDFAKVYWNSRLHTEHDRLVSLFRGEASSRERKQQERAKRENHEKSTETAVEPDNTPATAVCDVFAGVGPFAVPSGRTSLFVMANDLNPYSYEALEHNVKLNKVSEYVKCFNLDGAEYVQQSMKLLDEHRRTQPTINPVQVRKRKAGQPVVKQDIPNHYSHYVMNLPDSAIEFLWSFKGLYTTVDGLSQDTPLPHVHVHCFHKWEAGEEEPSKEETKAALLERVYKQIDVRLDPNEVGMHWVRKVSPKKDMFCISFELPS",
    "response#2": "FSMVGHVAHLNLRSEWKDYDRLIGQVILDKNPRVKTVVNKVDSIDTKFRTFKMDVLAGEDNTEVEQHESGCRFQFDFAKVYWNSRLHTEHDRLVSLFRGEASSRERKQQERAKRENHEKSTETAVEPDNTPATAVCDVFAGVGPFAVPSGRTSLFVMANDLNPYSYEALEHNVKLNKVSEYVKCFNLDGAEYVQQSMKLLDEHRRTQPTINPVQVRKRKAGQPVVKQDIPNHYSHYVMNLPDSAIEFLWSFKGLYTTVDGLSQDTPLPHVHVHCFHKWEAGEEEPSKEETKAALLERVYKQIDVRLDPNEVGMHWVRKVSPKKDMFCISFELPE",
    "response#3": "FSMVGHVAHLNLRSEWKDYDRLIGQVILDKNPRVKTVVNKVDSIDTKFRTFKMDVLAGEDNTEVEQHESGCRFQFDFAKVYWNSRLHTEHDRLVSLFRGEASSRERKQQERAKRENHEKSTETAVEPDNTPATAVCDVFAGVGPFAVPSGRTSLFVMANDLNPYSYEALEHNVKLNKVSEYVKCFNLDGAEYVQQSMKLLDEHRRTQPTINPVQVRKRKAGQPVVKQDIPNHYSHYVMNLPDSAIEFLWSFKGLYTTVDGLSQDTPLPHVHVHCFHKWEAGEEEPSKEETKAALLERVYKQIDVRLDPNEVGMHWVRKVSPKKDMFCISFELPEP"
  },
  {
    "reference": "MGYKLAIVGATGNVGRELLSILAERQFPADVVVALASSKSIGTEVSYGEDDTLSVQNLEGYDFTNTDIVLSSPGAKVSAQFAPKAAEAGAVVIDNSSYFRMEPDVPLVVPEVNSKDIAGYTKRGIIANPNCSTIQMVLALKPLHDHANIKRVVAATYQSVSGAGKDAMDELFNQTRGIFVNEPSETYRQNFTKQIAFNVIPHIDMFMDDGSTKEEWKMVAETKKILSPDIAVTVTCARCPVFIGHAEAINVEFERPISEKDARAALSATPGITVVDHRVDEGYVTPQECAGEDQVYVSRIRKDPTVENGLSIWVVADNLRKGAALNTVQIAEELVRSHF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that is capable of catalyzing reactions within the L-threonine from L-aspartate: step 2/5, L-threonine biosynthesis, Amino-acid biosynthesis pathway with high efficiency and specificity.2. The designed protein must have at least one Semialdehyde dehydrogenase NAD-binding domain.3. A protein with NAD binding, NADP binding, aspartate-semialdehyde dehydrogenase activity, protein dimerization activity is needed to enhance diaminopimelate biosynthetic process, 'de novo' L-methionine biosynthetic process, threonine biosynthetic process, lysine biosynthetic process via diaminopimelate, isoleucine biosynthetic process in a specific context.4. The Acyl-thioester intermediate active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.5. The protein should be able to bind NADP(+), phosphate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "VAIVGATGQVGAVMRRLLAERDFPVASVRYLASARSAGTTLAWAGSAAGAPGYDVARDIEVEDVATADLSGIDVALFSAGGGASKEHAPRFAEAGAVVVDNSSAWRMDDAVPLVVSEVNPDDLTNLDAPSGANIVVAGTCVRVPVFTGHLAPLHKRYGIDKVVVSTYQAVSGSGHKAIVELENQMKDANYPNNVYPKRIATNCIPHIDVFNDNGFTKEELKMVYETQKILEDNKIKVNSTTVRIPVFFSHSESVYVEFKETPEVSEVKQILSEAPGVIVLDNPEKNEYPTPLEVANSDDTYVGRIRKDLFNPNALSMWVVADNLRKGAALNTIQLGGLFRENLVEWTSAMTYQAASGAGAQNMRELITQMGQIRDSVATELADPASAILDIDRKVVEAMRSDLTVDRFGAPLAGSLLPYIDKQLENFQSKEEWKGGVETNKILGTSDSPIPIDGICVRIGAMRCHSQALTIKLKKDVPLADVESLIANANSWVKVIPNDRDATLEELTPTKVTGTLSVPVGRLRKMAMGPEYLSAFTVGDQLLGCPATIEAL",
    "response#2": "MSNVAVVGATGLVGSEFLKSLRSEYVKELLPIASENSKGKRIMFNQKEYDILTINEGLESKIDVAFFCAGSQISKNYAREFAKKGAIVIDNSSYFRMNDEIPLVVPEVNGEVLDDALAKHRGIIANPNCSTIQMVVALKPLHDAAKIRRVVVSTYQSVSGAGKAGMDELFNQTRGIFVNDQIVPDQFTKQMAFNVIPHIDVFMDDGHTKEEWKMRVETNKILDRDIKVNATCVRVPVFVGHAESVNVEFANELSVEQARGLLRAMPGVTVIDHRADEGYVTPVECAGDDMVYVSRIRKDPTIKHGLSFWCVSDNLRKGAALNAVQIAELLVARGIVK",
    "response#3": "LVGLVGWRGMVGSVLMQRMQEEGDFSHIEPVFFSTSNTGGKAPAMAKNETTLKDANDIEALKKCEIIITCQGGDYTSEIFPKLRAAGWNGYWIDAASTLRMKEDAIIVLDPVNLNVIKDALAKGGKNWVGGNCTVSLMLMGVGLRPLHEQAGLERLRVATYQAVSGSGLAGVTELADQARAALGGDLPVETLTHDGGAVAMPEPGVYVAPIAFNVLPLAGDLGADDETTEEIKLRNESRKILGIPGLAVSGTCVRVPVFTGHSLAVHAEFAEPITPDQARELLAQAPGVALADVPTPLQAAGKDPSLVGRIRVDQSVPDGRGLVLFISNDNLRWGAAEPLRRMLRILL"
  },
  {
    "reference": "MPPAAPVRLALALGLGLWLGALAGGPGRGCGPCEPPCLCGPAPGAACRVNCSGRGLRTLGPALRIPADATALDVSHNLLRALDVGLLANLSALAELDISNNKISTLEEGIFANLFNLSEINLSGNPFECDCGLAWLPRWAEEQQVRVVQPEAATCAGPGSLAGQPLLGIPLLDSGCGEEYVACLPDNSSGTVAAVSFSAAHEGLLQPEACSAFCFSTGQGLAALSEQGWCLCGAAQPSSASFACLSLCSSPPPPPAPTCRGRTLLQHVFPASPGAALVGPHGPLASGRLAAFHIAAPLPVTATRWDFGDGSPEVDAAGPAASHRYVLPGRYHVTAVLALGAGSALLGTDVQVEAAPATLELVCPSSVQSDESLDLSIRNRGGSGLEAAYSIVALGEEPARAVHPLCPSDTEIFPGSGHCYRLVVEKAAWLQAQEQCRAWAGAALAMVDSPAVQRFLVSRVTRSLDVWIGFSTVQGVEVGPAPQGEAFSLESCQNWLPGEPHPATAEHCVRLGPTGWCNTDLCSAPHSYVCELRPGGPVQDAENLLVGAPSGDLQGPLTPLAQQDGLSAPHEPVEVMVFPGLRLSREAFLTTLRLQVYRLLSTAGTPENGSEPESRSPDNRTQLVPACMPGGCWCPGANICLPLDASCHPQACANGCTSGPGLPGAPYALWREFLFSVPAGPPRAVLGVTLHGQDVLMLPGDLVGLQHDAGPSALLHCSPAPSHPGPQAPYLSANASSWLPHLPAQLEGTWACPACALRLLAATEQLTV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be involved in placenta blood vessel development, metanephric proximal tubule development by localizing to extracellular exosome and exhibiting protein kinase binding.2. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "ECEANEIKISLSKCQLQSLGFMKVFMYLNDRQCSGFSERGERDWMSIVTPARDGPCGTVLRRNETHATYSNTLYLASEIIIRDINIRINFECSYPLDMKVSLKTSLQPMVSALNISLGGTGKFTVQMALFQNPTYTQPYQGPSVMLSTEAFLYVGTMLDGGDLSRFVLLMTNCYATPSSNSTDPVKYFIIQDRCPHTEDTTIQVTENGESSQARFSIQMFRFAGNSDLVYLHCEVYLCDTMSEQCKPTCSGTRYRC",
    "response#2": "STCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGDVPPGPLPEAILALYNSTRDRVAGESAETEPEPEADYYAKEVTRVLMVEYGNKIYDKMKSSSHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLTRREEIEGFRLSAHCSCDSKDNTLQVDINGFSSGRRGDLATIHGMNRPFLLLC",
    "response#3": "STCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGDVPPGPLPEAILALYNSTRDRVAGESAETEPEPEADYYAKEVTRVLMVEYGNKIYDKMKSSSHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLTRREEIEGFRLSAHCSCDSKDNTLQVDINGFSSGRRGDLATIHGMNRPFLLLN"
  },
  {
    "reference": "MSSRFFYGGGSDSDSSSSDEEELYSDREEEEKSEEEESSEEEDETSEEEESDEETGARKFLKDVASDSEEEEEEEKVTVVKSAKDKRLDELENTIKLIENAKKINDWAVISTEFDKLNRQVAKITQSGPTPKIYIKAVADLEDFVNETVAKQKSGDKKLNASQAKGFNAAKQRIKKNNKDYGNLIDKYRKDKEDFMESDDEEAIPVIAAPRITKLERIEAPAAAIDDDGFATVGRGGKTLQYTPESILKHLRVIVESRGKKNTDRMEQIRTMEKLLEVAQTPYQRIRVYLTLISTRFDLTSTSSANYMAVDQWKSAEQDFSSLLSVLENNRDHVVFEGAEEWEDDEKQPTIAPGETLYIPGSIVSFAERLDDELTRSLQHIDPHTAEYIERLSDEKLLYTDLVRAQAYVEGLNEVEKTDPRQDSVNRVVMRRLEHVYFKPSQVITILEDATWKSLPSELDSSITPRASSGNVENLVLSLCNYLFKYSDGIIRARAMLCQIYFLALHDQYYRSRDLMLMSHLTENISNFDVSTQILFNRTLVQIGLCAFRSGLIYEAQNTLSEVCGSGRQKELLAQGIIMQRYSTVSPEQERLERQRQLPFHMHINLELLECIYLTSSMFLEVPLMAQTSSSPEMKRRVISKTFRRMLDYNERQVFTGPPENTRDGVIMSAKFLAAGDWKKAAEMLNSIKIWDLMPQPDKIKEMLSQQIQEEGLRTYLFTYAPFYDSLSIATLSNMFELSEKKISAIISRMISHEELAAALDQVNNAIV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.2. The protein should be optimized for stability and solubility when expressed in eukaryotic 43S preinitiation complex where it will be involved in translational initiation. The designed protein sequence is ",
    "response#1": "FHMHINLELLECVFMTSSLLIEIPAMAAVSSSSKDSKRKASLKSFKSKLDFHERQYFTGPPESIKDHVVHSSIALQKGDWAKAYKLLSSIKIWKLIPDNEQLLSMMKKQLQVEGLRTYIFTYKSIYTKLSIAKLSAIFDIEKESVYSIIDKMIQPGDISGSIDESKSFVNFL",
    "response#2": "FHMHINLELLECVFMTSSLLIEIPAMAAVSSSSKDSKRKASLKSFKSKLDFHERQYFTGPPESIKDHVVHSSIALQKGDWAKAYKLLSSIKIWKLIPDNEQLLSMMKKQLQVEGLRTYIFTYKSIYTKLSIAKLSAIFDIEKESVYSIIDKMIQPGDISGSIDESKSFVNFI",
    "response#3": "FHMHINLELLECVFMTSSLLIEIPAMAAVSSSSKDSKRKASLKSFKSKLDFHERQYFTGPPESIKDHVVHSSIALQKGDWAKAYKLLSSIKIWKLIPDNEQLLSMMKKQLQVEGLRTYIFTYKSIYTKLSIAKLSAIFDIEKESVYSIIDKMIQPGDISGSIDESKSFVNFL"
  },
  {
    "reference": "MKKFRFFVSGMPGVGKTTLAKRIADEIKREGFKVGGIITQEIRSGARRSGFRVIALDTGEIGRLAYVGQGYPRLGRYVIDVESFEKVAIPAISRALREGDLIVIDEIGPMEFKSNEFLKALGLVLRSEKPLLATVHRRFVERYRPLGEYYWLTPENREAVFSEILVKIKELLRENENAGNKAQD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a high affinity for ATP.2. The protein must exhibit the following characteristics: May hydrolyze nucleoside diphosphates with lower efficiency..3. The protein must exhibit ATP binding.4. The protein should have a low Km value for a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-   diphosphate + H(+) + phosphate. The designed protein sequence is ",
    "response#1": "LQRTLSIIKPDAVEKNAEGDIISILLNNNFRILGIKMLHLTKNQAEGFYAVHANKPFFHSLTDFMASGPIVVMCLEKENAIADYRKLMGATNPANAEEGTIRKKWAASIEKNAVHGSDADDTARFELSYFFAGYELAR",
    "response#2": "FETTFVMLKPGVLQRRLVGEVLSRFERKGLVLTALRLLCVDTATAELHYAEHREKPFYPSLIAYITSAPVVALAFKGENAISLVRTLCGSTRVEHAQPGTIRGDFALRTTTNIVHASDSPESAARELALYFSAQDFVS",
    "response#3": "MERTYVMVKPDGVQRCLVGEIVSRIEKKGLKIAALRMNVMTEAAAKEHYKEHSERPFFGSLVSFVTSGPSVSMVIEGNNAIKIMRAINGATNPVDALPGTIRGDLAVDMGRNVVHASDAPESAEREIGLHFEESEISH"
  },
  {
    "reference": "MDKLKLLVINNYGQTCHLIHRSLRDLGMDAKLVSNTSTVEDILKQSPDGLVFSGGPTMERAGNGENLIKIDLPILGICLGHQLMAKAYGGEIRTGQYGGFARVDIDIVEEDDILRGIGPKTVVWASHQDEVAKLPPDFVLLAKSNICDIEAMRHKTKPLFGVQWHPEVSHTEKGTLLLQNFIEVCRQRK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Catalyzes the synthesis of GMP from XMP..2. The designed protein should be optimized for ATP binding, GMP synthase (glutamine-hydrolyzing) activity activity in order to improve glutamine metabolic process.3. The protein design should consider the endogenous proteins and enzymes present in the GMP from XMP (L-Gln route): step 1/1, Purine metabolism, GMP biosynthesis pathway to avoid interference with native metabolic processes.4. The protein contains novel Glutamine amidotransferase type-1 domains that confer a unique function or activity.5. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile to enable effective catalysis of ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2   H(+) + L-glutamate. The designed protein sequence is ",
    "response#1": "IKNKVNDEKVILGLSGGIDSFTSAILIHKAIGNNLFCICIDNGLLRNDEILKIKNLIKKVGKINVIYINHKKRFLKSLNGITDPEKKRKTIGNLFFKIFQEQADILKAKWLAQGTIYPDIIESSQNNLLKKDNFIKSHHNVCPLPKGIKLKILEPLKHLFKDEVKKIAKKIGIPKEIIFRHPFPGPGLAVRIIGEIKEEYCNILRMADEIFISELKSENLYFNISQAFSVLLPIKSVAVMGDMRKYEWVISLRAIETLDFMSANWANIPYKILNNVSNRIINEVRGISRVVYDISNKPPSTIEWITKLVKDLKNQIGSANVLLGLSGGVDSSVVAILLQQAVGKQLTCVFVDNGLLRLNEGNKIMQTFAQNMSVKVIRVNAQEKFYNALSNEDRFEELKSSGLYQQVWQAFSVLLPVQSVGVMGDKRTYENVLALRAVESTDGMTADWAHLPHDFLAKVSNRIINEVRGINRVAYDISSKPPATIEARKAKYDYIDKLL",
    "response#2": "IRDQVGDGRIICGLSGGVDSSVAAVLIHEAIGDQLTCVFVDHGLLRLNEAEQVVTMFRDHYNIPLVHVDAAEEFLGALAGVTDPETKRKTIGRLFVEVFDREAAKIEGAAFLAQGTLYPDVIESVSARGGPSAVIKSHHNVGGLPDYMKLKLVEPLRELFKDEVRALGVELGLPPQFVGRHPFPGPGLAIRIPGEVTPERVAILQKADAIYLDEIRKAGLYDSIWQAFAVLLPVRTVGVMGDARTYDEVCALRAVTSTDGMTADFFEFPWEVLSRAATRIINEVRGINRVVYDVTSKPPGTIEWRHIEEMGAAGLDRKVWQCPVVLLSDVHSVGVQGDGRTYGSPIVLRPVTSEDAMTADWARIPYDVLEKISTRITNACPQINRVVLDITSKPPATIEIRAQVGTGKVICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGLLRKDEGKSVVDLFRHHYNIPLVHVDVSETFLGALKGVTDPEQKRKTIGKLFIDVFEAEARRVGGADFLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPERMNMKLVEPLRELFKDEVRALGRELGLPDVFVGRHPFPGPGLAIRCPGEITEEKLEILRNADAVYIDQIRKAGLYDVIWQAFAVLLPVRSVGVMGDGRTYDYVVGLRAVTSTDGMTADFYPFEMSFLGATATRIINEVKGVNRVVYDVTSKPPGTIEWRTGADKAAVLIVDFGSQVTQLIARRLRESGVYCEIHPYDKVDAVLDSFRPKAVILSGGPASVHEAESPAASKRLFELDVPVLGICYGEQTMCDVLGGKVEPGTTREFGRAEIEIVRESPLLDGLGGAGHFEPVWMSHGDKVTALPMGFETVAVSEGSPFAVIADEGRRFYGIQFHPEVAHTPRGALILRNFTHKIA",
    "response#3": "IRDQVGDGRIICGLSGGVDSSVAAVLIHEAIGDQLTCVFVDHGLLRLNEAEQVVTMFRDHYNIPLVHVDAAEEFLGALAGVTDPETKRKTIGRLFVEVFDREAAKIEGAAFLAQGTLYPDVIESVSARGGPSAVIKSHHNVGGLPDYMKLKLVEPLRELFKDEVRALGVELGLPPQFVGRHPFPGPGLAIRIPGEVTPERVAILQKADAIYLDEIRKAGLYDSIWQAFAVLLPVRTVGVMGDARTYDEVCALRAVTSTDGMTADFFEFPWEVLSRAATRIINEVRGINRVVYDVTSKPPGTIEWRDGHFFGRETLEGWTAANIAEELIEKVREQIGEDGRAICGLSGGVDSAVAGALVQRAIGDRLTCVFVDHGLLRAGEREQVEKDFVAATGAKLVTVDERQAFLSKLAGVTEPEAKRKAIGAEFIRSFERAVAGVLEEAPEGSTVDFLVQGTLYPDVVESGGGSGTANIKSHHNVGGLPDDVEFKLVEPLRDLFKDEVRAVGRELGLPEEIVGRHPFPGPGLAVRILGEVTPERLNILRDADWIVRDEMKKHGYYNKVWQSFAVLLPIQTVGVMGDFRTYEHVVALRVVDSRDAMTADWSRVPYDILGDISTRIINEVRGVNRVVYDISSKPPATIEWE"
  },
  {
    "reference": "MLIDTSKFDQCHLLVVGDLMLDEYLWGEVDRISPEAPVQVVSIKNEDFALGGAGNVVNNVVALGGKVSTVGVIGTGRNGQLLLKIFKKLGVDTAGIVKEPGRTTTQKTRIIAANQHVLRIDRETQQDISASSLEKIARFIEDKMIDIDVVLISDYGKGLITESMLSRVIASAKKHKKMIIVDPKGLDFSKYSGVSLLTPNKKEAALASGVEIIDESSLEKAAHKILKHISLDNLLITCGKDGMVLFERNKEPFRVKAEARQVFDVSGAGDTVLTVLGLAVASGTSINSGVAVANTAAGIVVGKVGTATVSKQELASALKSHDHGLPAKYKDFSELPALAEDLKKKGKRLVMTNGCFDLLHAGHIMLFSASKQLGDILIVAIDDDESVRILKGSGRPVLNEKERSRILSALDAVDYVVVFSSHELKKLIEIIQPDVLTKGSNYTSKEVFGRELVEKYGGRVELIPVTEKISSTRIINNIKSSRQE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7- phosphate at the C-1 position to selectively form D-glycero-beta-D- manno-heptose-1,7-bisphosphate..2. The presence of Cytidyltransferase-like, Carbohydrate kinase PfkB domains is critical for the interaction of the designed protein with its intended targets.3. A protein that is capable of catalyzing reactions within the ADP-L-glycero-beta-D-manno-heptose from D- glycero-beta-D-manno-heptose 7-phosphate: step 3/4, Nucleotide-sugar biosynthesis, ADP-L-glycero-beta-D-manno- heptose biosynthesis pathway with high efficiency and specificity.4. The designed protein is the optimization of the active site to achieve high catalytic efficiency for ATP + D-glycero-beta-D-manno-heptose 7-phosphate = ADP + D-   glycero-beta-D-manno-heptose 1,7-bisphosphate + H(+).5. The protein must be able to heptose-1-phosphate adenylyltransferase activity, phosphotransferase activity, alcohol group as acceptor, heptose 7-phosphate kinase activity. The designed protein sequence is ",
    "response#1": "VLCLGDLILDTFNHGSVERISPERPVPVFRPGQVVHIPGGAANVARNITSLGGHCSLVGLAGHDEASSILHDLLQSDARISCNLVFSERPTCHKVRFTAAGQHLMRLDNETNDPIGNVEIQRVLSLLEPLLPGHDVLILSDYAKGLLCPDLLQQVIAMARRFRIPVVVDPKSPDFNSYAGASLLTPNTVEAERATGFPIHTTEDAERAGLRLLNESGVSAVLITRGAHGMSLVLDDAPPLHLASGALEVFDVVGAGDTVIATLACVLAAGGSYGEAASTANVAAGLVVAKPDTATALAAPPAAGPLFLDVLVAGDVMLDRYWHGATSRISPEAPVPVVRVDTDESRVGGAGNVALNITALGAGATLAALVGEDEAAETLRSQLGAGRVVCRLLASASSRTITKLRVLSRHQQLIRLDFEDAFPAEAAAAMPDAVASALHGAGALVLSDYAKGALAQAAQMIAAAREAGVPVVVDPKGTDFARYRGASVLTPNLTEFEAVVGACADDDTLVQRGEALRDALGLEALLITRSERGMTLLERAQPPLHLPTRAREVFDVTGAGDTVVAVLAAGLAAGLGLPEATALANLAAGVVVGKLGTATVSLDELRRHLRTSGPAPGSDRLERWRLKGYRIGFTNGCFDLLHPGHVSLLEQAKAHCDRLVVGLNSDQSVKRLKGPSRPVQSEAARATVLSSLGSVDLVVVFDQDTPLDLITRLRPDLLVKGADYAVAQVVGADLVQGWGGKVILAQLAEGHSTTATIARMRGRAA",
    "response#2": "VIGDAMLDTYFEGTAARLCSEGPVPIVRKTSERHLPGGAANTAANLRALDAQVMLLGFVGRDNAGNLLRAALRERGIDDTWLIEDERASTLHKLRILADGQFVVRFDDEDAHHYAPPGQQLLLARLEEMFPRCDVVIVSDYCYGVISDALIDRLQFLHAAHPNVLLAVDSKNLRRFKHAGATVITPNLLEAQLLVTQNDDMQGLLPAVPEQASTPFPEAERIGQRLLSMIDAEHAVITMAADGVMLVDRQGRARHIPAHPVHHANDIGAGDSFVAAMALSLAAGAQLDEAARIGIDAAEIAVTKRWDVERQRGRTIVFTNGVFDILHAGHVHLLRQAKQLGDVLVVGVNSDQSAQRLKGKHRPINSERDRLALVAALDPVDYVALFDEDTPATLIRTLRPHIHVKGGDYADEQLPEAGAVHEVGGRIVIIPLVGSMSTSSVIDRILEGS",
    "response#3": "VLCLGDLILDTFNHGSVERISPERPVPVFRPGQVVHIPGGAANVARNITSLGGHCSLVGLAGHDEASSILHDLLQSDARISCNLVFSERPTCHKVRFTAAGQHLMRLDNETNDPIGNVEIQRVLSLLEPLLPGHDVLILSDYAKGLLCPDLLQQVIAMARRFRIPVVVDPKSPDFNSYAGASLLTPNTVEAERATGFPIHTTEDAERAGLRLLNESGVSAVLITRGAHGMSLVLDDAPPLHLASGALEVFDVVGAGDTVIATLACVLAAGGSYGEAASTANVAAGLVVAKPDTATVLVAGDVMLDRYWHGATSRISPEAPVPVVRVDTDESRVGGAGNVALNITALGAGATLAALVGEDEAAETLRSQLGAGRVVCRLLASASSRTITKLRVLSRHQQLIRLDFEDAFPAEAAAAMPDAVASALHGAGALVLSDYAKGALAQAAQMIAAAREAGVPVVVDPKGTDFARYRGASVLTPNLTEFEAVVGACADDDTLVQRGEALRDALGLEALLITRSERGMTLLERAQPPLHLPTRAREVFDVTGAGDTVVAVLAAGLAAGLGLPEATALANLAAGVVVGKLGTATVSLDELRARAEGRPAEFWLSEWRRQGLRVGFTNGCFDILHPGHVKVLTAARGACDRLIVGLNSDASVRRLKGQGRPVQDERARAEVLAALEAVDLVAIFEEDTPINLITAIKPSVLVKGGDYTRAQVVGHEIVEANGGEVLLVDILPGFSTTSLVARARQHEG"
  },
  {
    "reference": "MINGVYYNEISRDLDISSSTQCLRFLKETVIPSLANNGNNSTSIQYHGISKNDNIKKSVNKLDKQINMADRSLGLQQVVCIFSYGPHIQKMLSILEIFKKGYIKNNKKIYQWNKLTSFDIKREGRNELQEERLKVPILVTLVSDSEIIDLNLHSFTKQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends2. The designed protein should be involved in intronic box C/D RNA processing, nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay by localizing to cytosol, telomerase holoenzyme complex, nucleolar ribonuclease P complex, ribonuclease MRP complex and exhibiting RNA binding. The designed protein sequence is ",
    "response#1": "AGLEPHIPNAYCNCMIQVLYFLEPIRCLVQNHLCQKEFCLACELGFLFHMLDLSRGDPCQASNFLRAFRTIPEASALGLILADSDEQTGKAKLGRLIQSWNRFILTQLHQETQEQEGPQAYRGVSSSSLGSSAESAIGKLFGCEVENSSLCRCGKETVRSSLTLLFTMHYPEQNSQDKTIKEYDFAEILKRSICLEQNTQAWCENCEKYQPTVQTRNIRCLPDVLVINCEVNSAKEAEFWKVQAECDLA",
    "response#2": "AGLEPHIPNAYCNCMIQVLYFLEPIRCLVQNHLCQKEFCLACELGFLFHMLDLSRGDPCQASNFLRAFRTIPEASALGLILADSDEQTGKAKLGRLIQSWNRFILTQLHQETQEQEGPQAYRGVSSSSLGSSAESAIGKLFGCEVENSSLCRCGKETVRSSLTLLFTMHYPEQNSQDKTIKEYDFAEILKRSICLEQNTQAWCENCEKYQPTVQTRNIRCLPDVLVINCEVNSAKEAEFWKC",
    "response#3": "AGLEPHIPNAYCNCMIQVLYFLEPIRCLVQNHLCQKEFCLACELGFLFHMLDLSRGDPCQASNFLRAFRTIPEASALGLILADSDEQTGKAKLGRLIQSWNRFILTQLHQETQEQEGPQAYRGVSSSSLGSSAESAIGKLFGCEVENSSLCRCGKETVRSSLTLLFTMHYPEQNSQDKTIKEYDFAEILKRSICLEQNTQAWCENCEKYQPTVQTRNIRCLPDVLVINCEVNSAKEAEFWKVQAECDLS"
  },
  {
    "reference": "MTVKKIAIFGATGRTGLTTLAQAVQAGYEVTVLVRDSSRLPSEGPQPAHVVVGDVRQAADVDKTVAGQEAVIVLLGTGNDLSPTTVMSEGTRNIVTAMKAHGVDKVVACTSAFLLWDPTKVPPRLQDVTDDHIRMHKILQESGLKYVAVMPPHIGDQPLTGAYTVTLDGRGPSRVISKHDLGHFMLRCLTTNEYDGHTTYPSHQYD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have biliberdin reductase NAD+ activity, biliverdin reductase (NADP+) activity, oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor, riboflavin reductase (NADPH) activity, biliverdin reductase (NAD(P)+) activity within intracellular membrane-bounded organelle, plasma membrane, terminal bouton, nucleoplasm, cytosol to regulate heme catabolic process.2. The protein should have a low Km value for bilirubin IXalpha + NADP(+) = biliverdin IXalpha + H(+) +   NADPH.3. The designed protein should have a high specificity for NADP(+), substrate binding, with minimal off-target effects.4. For general function, the protein need meet that In the liver, converts biliverdin to bilirubin.; Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH; Broad specificity oxidoreductase that catalyzes the NADPH- dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone); Contributes to heme catabolism and metabolizes linear tetrapyrroles The designed protein sequence is ",
    "response#1": "ALHGISRITNSMKGVHTVYHAPQGDDYITATYSMLERTPDFPGLSISVVRGRDLAQGVSRLPGTLQQVAHHYSPDLTVIAPSCSTALLQEDLHQLAAHSGVPEEKLLVYALNPFRVSENEAADGLFFELVKRFAVAQEKTPRPSVNLLGFTSLGFHLRANLTSLRRMLQTLGVSVNVVAPWGAGIEDLRKLPAAWLNIAPYREIGETAAGYLGETFGMPAIHEAPIGVEPTLAWLRSVIEKINAVGVEQGVPPIGMPKLNAFSLDGMSAPSGVPWFARTADMESFSNKRAFVFGDATHTVSIVKFLRDELGMKIIGAGTYMHRHADFVRRELEGYLSGELLVTDKFQDVSKVIEDEMPDLVCGTQMERHSCRKLDVPCMVICPPTHIENHLLGYYPFFGFDGADVIADRVLKGLQKLEPFLFEFLRREVLGKELLKGSLRFTASPLEEERFGFPAFSGISRLTWLVSLFGELSLISATLEERKEDQYMKMTVQLETQNKGLLSWIEEKGPGLKRNRYVNFQFTSGSLEEVPPLPPGLTFDPPDWPPGGATGRTGLTTLAQAVQAGYEVTVLVRDSSRLPSEGPQPAHVVVGDVRQAADVDKTVAGQEAVIVLLGTGNDLSPTTVMSEGTRNIVTAMKAHGVDKVVACTSAFLLWDPTKVPPRLQDVTDDHIRMHKILQESGLKYVAVMPPHIGDQPLTGAYTVTLDGRGPSRVISKHDLGHFMLRCLTLLL",
    "response#2": "GATGNTGLTTLAQAVQAGYEVTVLVRDPSRLPSEGPQPAHVVVGDVRQPADVDKTVAGQDAVIVLLGTRNDLSPTTVMSEGAQNIVAAMKAHGVDKVVACTSAFLLWDPSKVPPRLQDVTDDHIRMHKVLQQSGLKYVAVMPPHIGDHPLTGAYTVTLDGRGPSRVISKHDLGHFMLHCLTGNAVVADAFFEFLRREVLGKELLKGSLRFTASPLEEERFGFPAFSGISRLTWLVSLFGELSLISATLEERKEDQYMKMTVQLETQNKGLLSWIEEKGPGLKRNRYVNFQFTSGSLEEVPDVETLIQPTLPDFEFLRREVLGKELLKGSLRFTASPLEEERFGFPAFSGISRLTWLVSLFGELSLISATLEERKEDQYMKMTVQLETQNKGLLSWIEEKGPGLKRNRYVNFQFTSGSLEEVPDAETLIQPTLPDTQALVWTDEAENMLKKVPFFVRKKVRKNTENFAREQGETTVTADVFRQAFGG",
    "response#3": "ALHGISRITNSMKGVHTVYHAPQGDDYITATYSMLERTPDFPGLSISVVRGRDLAQGVSRLPGTLQQVAHHYSPDLTVIAPSCSTALLQEDLHQLAAHSGVPEEKLLVYALNPFRVSENEAADGLFFELVKRFAVAQEKTPRPSVNLLGFTSLGFHLRANLTSLRRMLQTLGVSVNVVAPWGAGIEDLRKLPAAWLNIAPYREIGETAAGYLGETFGMPAIHEAPIGVEPTLAWLRSVIEKINAVGVEQGVPPIGMPKLNAFSLDGMSAPSGVPWFARTADMESFSNKRAFVFGDATHTVSIVKFLRDELGMKIIGAGTYMHRHADFVRRELEGYLSGELLVTDKFQDVSKVIEDEMPDLVCGTQMERHSCRKLDVPCMVICPPTHIENHLLGYYPFFGFDGADVIADRVFEFLKREVAGKELLKGSLRFTASPLEEEKFGFPAFSGISRLTWLVSLFGELSLISATMENRKEDQYMKMTVQLETQNKSPLSWIEEKGPGLKRNRHISIHFKSGSLEEVPTFVWFTALHGISRITNSMKGVHTVYHAPQGDDYITATYSMLERTPDFPGLSISVVRGRDLAQGVSRLPGTLQQVAHHYSPDLTVIAPSCSTALLQEDLHQLAAHSGVPEEKLLVYALNPFRVSENEAADGLFFELVKRFAVAQEKTPRPSVNLLGFTSLGFHLRANLTSLRRMLQTLGVSVNVVAPWGAGIEDLRKLPAAWLNIAPYREIGETAAGYLGETFGMPAIHEAPIGVEPTLAWLRSVIEKINAVGVEQGVPPIGMPKLNAFSLDGMSAPSGVPWFARTADMESFSNKRAFVFGDATHTVSIVKFLRDELGMKIIGAGTYMHRHADFVRRELEGYLSGELLVTDKFQDVSKVIEDEMPDLVCGTQMERHSCRKLDVPCMVICPPTHIENHLLGYYPFFGFDGADVIADRV"
  },
  {
    "reference": "MDSRQYAENIAKKAKNTVRKLSSLTTKTKNDALLRTAELLLERKSQIIEENKKDLELAEKKGYSKALLDRLALDDKRINQMVQVLKDVAALPDPVGEIINMWTRPNGLKVGQMRVPLGVILIIYEARPNVTIEAASLCMKSSNAVILKGGSETINSNRILVDIIKQACRETCFPEEAVQFVDTTDREVVNHLLKLEGLIDVAIPRGGESLIRAVAENSKIPVIKHYKGVCNLYVDDEADMEKALNIAYNAKVQRPSVCNAIENLVVHKKIADKFLPEIAYYFGKAGVEMRCDEYSYNLLINHPKAKDTEIVPAKEEDYYEEFLDLIIAVKVVDSLDEAIDFIEKYGSHHSDAIVTENYTKGMKFIQDVDSAAVYINASTRFTDGNEFGLGAEMGISTDKIHARGPMALKELTIPKFIVFGNGQLRENVGIPKDESEIKIDTNACSL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design should consider the endogenous proteins and enzymes present in the L-glutamate 5-semialdehyde from L-glutamate: step 2/2, L-proline biosynthesis pathway to avoid interference with native metabolic processes.2. The designed protein should have NADP binding, glutamate-5-semialdehyde dehydrogenase activity within cytoplasm to regulate L-proline biosynthetic process.3. We are looking for a protein with broad substrate specificity for L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + L-   glutamyl 5-phosphate + NADPH.4. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent reduction of L-glutamate 5- phosphate into L-glutamate 5-semialdehyde and phosphate. The designed protein sequence is ",
    "response#1": "MKDMGRKARRAATVLAVMSSEQKNKALEMIALSLEAHSDEILRANHQDLLNATQNNLTVAMVDRLKLNEVRLCTIIDSVRQVACLPDPVGQVMDEWTRPNGLHISRVRTPLGVIGIIYESRPNVTIDASTLCLKAGNAAILRGGSDSFHSAHALHCALVKGLKKAGFPKDAIQMVATKDRGAVGEMLKGLDGAIDVIVPRGGKNLVARIQVDARVPIFAHLEGLCHIYIDQSADLDMARNIVLNAKLRRTGICSAVETVLIDRQALEKFLPILTALQKKGCEIRATEDIVFLMPDAKLAFEEDWSHEYLDAIVSVKTVESVEGAIAHIKRYSSGHTESIIAEDMEIVEKFFNHLDSAILLHNASTQFADGGEFGFGAEIGIATGKMHARGPIGVEQLTSFQYHIKGNGQVRT",
    "response#2": "MQAVGQQARKASRVLSSASTHTKNNALSAIYTALVNHEPEILAANAVDMQKGRDSHLDSALLDRLELTPTRFKGMLQGLKDVIALTDPIGEITDLAYRPSGIQLGKMRVPLGVIGMIYESRPNVTLEAASLALKSGNAIILRGGSEALQSNKAIATAIQHGLKVAGLPEHAIQVIETADRAAVGQLITMTEYVDVIVPRGGKSLIERISNEARIPVIKHLDGNCHVFIDVEADLHKALPIALNAKTHRYGVCNAMETLLVHEEIAEEFLPRIAELYADKAEQLRLASIKTSQTNNNERIRALNLMADSLEKNSKEIIDSNFEDYKKAEIKGISKALLSRLKLSKEKLNLGIEGIRKVGDLSDPLGQIQIKKELSKGLILERKTVPIGVLGVIFESRPDAVMQISSLAIRAGNGVMLKGGSEANFTNQAIVSALKKGLQKSNIDDNAICLLTSRKDSMAMLNLEKFINLIIPRGSNELVKFIQENTGIPVLGHADGICHLYIDDEVNLDIALKVALDSKIQYPAACNAIETLLIHKSIAPAFLKKAIPIFNSNNVKLIGDKKAVKLGVAFEANYEDWQTEYLDLILSIKIVDDLEEGIAHIQKFSSKHTDGIITENISNANKFMSEIDSAGVFHNCSTRFADGFRYGFGAEVGISTQTLPPRGPVGLEGLVTYKYFLRGE",
    "response#3": "MARLSSAAKNELLLRMAEALENNAPLITAENARDLEAGQQKGLSAAMLDRLMLDESRIKAMADGLREVAALPDPVGEVTHMWKRPNEITVGKMRIPLGVIGIIYESRPNVTSDAAALCLKAGNAVVLRGGSEAIHSNLAIAGLLQDQMRKLGIPVAALSLIPFPERAGVTEMLKQEEFIDVIIPRGGESLIRFVVEHSKIPVIKHYKGVCHIFVDATADFGMAEKIIINSKTQRPGVCNALETLLIHKDVAETFVPRIHEALSTLKVELRGDEAFRQFAPGVKAATEEDWYAEYLELILAARVVDDLDEAIAHINKYGSLHTEAIITSDYANSQRFLREVNSSVVLVNASTRFSDGNQLGLGAEIGISTTKLHSFGPMGLEDLTTTKFIVYGR"
  },
  {
    "reference": "MSSNNESIKNWVSDKIFDILGYRESTMVDYIIALSKKAKDVNSFISTLTEQDFPINSNTKSFAQELLNKSQQKIQNITSSSSSSSSTSLSSSSSSKDKEKEKIEFLKKNKSYKLVIDHDDDIVNSSGSSDSDSDSERKRKKKEKKKEKKDKKDKKDKKSSTRKKSDNNWDDEIEPEPIKPNEKEDENNNNENNDNNNDNNNLQKRQPYKSIIEEENNNDNNNNNGEEDEYEREQREVKELSDRIKKRDEKSTKKKIVDDSETKESIERKNRLEQNEQLETERTKSRRKYLVGEEQKRLILLKREIEEEYELFKDQKLTEQEIKDFEKKKKLYELASQRINESQQSDDYYQLPSEIKDKDSLLKSSYINDNKNKKGNDSSSSSSYNPEQKEWEQNRMKSAISENRGLSTANIGGGNEEYEYVFEDQIEFIKEEVLKQGQKGDGVMILKPGDDGSAQAKMTIQEVRKSLPVYPYREQLIDAVREYQVLIIVGETGSGKTTQIPQYLHEAGFSKTGKIGCTQPRRVAAMSVAARVAEEVGCKLGNEVGYSIRFEDCTSQKTVLQYMTDGMLVREFLTAPDLASYSVLIIDEAHERTLHTDILFGLLKDITRFRPDLKLLISSATMDAERFSDYFDGAPTFNIPGRKYEVTTHYTQAPEADYLDAAVVTVLQIHITEPLGDILVFLTGQEEVDQAAEMLQTRTRGLGTKIKELIITRIYSTLPTDLQAKIFEPTPPNARKVVLATNIAETSLTIDGIIYVIDPGFCKQKMFN",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design must be able to perform ATP hydrolysis activity in order to contribute to RNA splicing.2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.3. The protein design must feature the DEAH box motif. The designed protein sequence is ",
    "response#1": "GFEKPMPIQAQALPIIMSGRDCIGIAKTGSGKTLAFVLPMLRHVKDQPPVVPGDGPIGLIMAPTRELVVQIHSDIKKFAKSLGINCVAIYGGSGVAQQISELKRGAEIVVCTPGRMIDILCTSSGKITNLRRVTFLVMDEADRMFDMGFEPQITRIVQNTRPDRQTVLFSAIFPRQVEILARKVLTKPVEIQVGGRSVVNKDITQFLTTRVIDLIMPIGKGQRALIVSPPKAGKTTILQDIANAITRNNLECHLMVVLVDERPEEVTDMQRSVKGEVIASTFDRPPSDHTSVAELAIERAKRLVEQGKDVVVLLDSITRLGRAYNNASPASGRILSGGVDSTALYPPKRFLGAARNIEEGGSLTIIATAMVETGSTGDMVIFEEFKGTGNAELKLDRKIAERRVFPAVDVNPSGTRKDELLLSPDEFGIVHKLRRVLSGLDSHQAIDLLMSQLADAEVAARLPVRAADAHKGTAGRVWIVGGHPGTVGAPALAGVGALRAGAGLVTVHSAAEVPLVMPELMVRRHADLAGALADTLPDARPDALALGMGLGPDAPALARLVLGWRIPTVLDADALQPELTGAGHDACVWTPHPGEAARLLGTRTPEITRDPLEAARALQARYGGVVVLKGGPSVVAHPGGVQVARPGHPGMASAGMGDTLSGVIAALLGQGLDAAGAALAGVSLHARAGQLAGAQHGYGLSATDVSVALGAAWLDLSA",
    "response#2": "NGAQAFVSAQTSTIPKTAFTAHDFVDYTLPQEQKGKEQKEQQWMKNSQTKDTDVHPYADFAVLEILLFPDSSTDRKIFNHFIQPAIRAYKQLGDSLNIFANQTLDQPKHNRYYLLGYPFLRNKAASLFLNQTEQRKEHMDKTTQILHEKPFVVLTDPAKPTLIRNKGANFTGNTWTSNYDLSKKSGLYHKFLGKKYQIYGKSIVISDLNLSSGSSGSLLLNDRKQIVGIYFR",
    "response#3": "NGAQAFVSAQTSTIPKTAFTAHDFVDYTLPQEQKGKEQKEQQWMKNSQTKDTDVHPYADFAVLEILLFPDSSTDRKIFNHFIQPAIRAYKQLGDSLNIFANQTLDQPKHNRYYLLGYPFLRNKAASLFLNQTEQRKEHMDKTTQILHEKPFVVLTDPAKPTLIRNKGANFTGNTWTSNYDLSKKSGLYHKFLGKKYQIYGKSIVISDLNLSSGSSGSLLLNDRKQIVGIYFR"
  },
  {
    "reference": "MTSIAVIGPGAVGGTLAAWLAQKPDHVVTVCVRTPFEALAVETPEGAISATPRVATSPESLAPVDWVLVTTKTYDTDATWTWLDALVGPQTRVAILRNGVEHVAPFVGKIAAERLVPAVVDIPAERSAPGRMLQRRNGWIKVPVGPAGEAFAALFAHTPIELHVVEDFVTEAWKKLALNCAGAVNALVLKPAGIAHDEGAAQVMRSLVRECVAVGRAEGADLSDDLPDQVIAGYRAADPGSVNSLHADRAAGRAMELDARNGVIVRRGAAHGIATPANAMVVALLNAAAL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that contributes to pantothenate biosynthetic process by functioning in cytoplasm with 2-dehydropantoate 2-reductase activity.2. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid..3. The protein should be designed to have high stability and activity towards (R)-pantoate + NADP(+) = 2-dehydropantoate + H(+) + NADPH.4. The protein design should take into account the physiological conditions of the (R)- pantoate from 3-methyl-2-oxobutanoate: step 2/2, Cofactor biosynthesis, (R)-pantothenate biosynthesis pathway.5. The NADP(+), substrate binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "IIGVAGSVAVGKSTTARVLQALLARWSPKPKVDLITTDGFLHSNAYLERAGLMDKKGFPESYNLGALLTFLSDIKAGRRDVRAPVYSHLTYDIVPNETIEIDQPDILIVEGLNVLQTGRLPADGKAVPFISDFFDFSVYIDADEGYEVFKVNQCEKLVINACINPTTATLDCVNGELYNDESAKELFRCIIKECVDIFFKCIPLFKNNEEAEKILNVNRLLDRVMFVGTKVNGANSSSTRQDCLLLRETEIDAINGYVVKLAENNGFQATVNKTMMLLTNL",
    "response#2": "IIGVAGSVAVGKSTTARVLQALLARWSPKPKVDLITTDGFLHSNAYLERAGLMDKKGFPESYNLGALLTFLSDIKAGRRDVRAPVYSHLTYDIVPNETIEIDQPDILIVEGLNVLQTGRLPADGKAVPFISDFFDFSVYIDADEICAKIIGNQCEKLVINACINPTTATLDCVNGELYNDESAKELFRCIIKECVDIFFKCIPLFKNNEEAEKILNVNRLLDRVMFVGTKVNGANSSSTRQDCLLLRETEIDAINGYVVKLAENNGFQATVNKTMMLLTR",
    "response#3": "IIGVAGSVAVGKSTTARVLQALLARWSPKPKVDLITTDGFLHSNAYLERAGLMDKKGFPESYNLGALLTFLSDIKAGRRDVRAPVYSHLTYDIVPNETIEIDQPDILIVEGLNVLQTGRLPADGKAVPFISDFFDFSVYIDADEIRVKEANQCEKLVINACINPTTATLDCVNGELYNDESAKELFRCIIKECVDIFFKCIPLFKNNEEAEKILNVNRLLDRVMFVGTKVNGANSSSTRQDCLLLRETEIDAINGYVVKLAENNGFQATVNKTMMLLTELR"
  },
  {
    "reference": "MHQTDAGLEVRALLPDATEVWVIEPKTGRKVAQLDCLDFRGFFCGLIPRRKNTFRYQLAVVWHGQENLIDDPYRFGPLIQELDSWLLSEGTHLRPYETLGAHAETMDGVTGTRFSLWAPNARRVSVVGQFNYWDGRRHPMRLRRESGIWELFIPGAHSGQLYKFELLDAEGQLRIKADPYAFEAQMRPETASLICGLPEKTTLSPERQKANGFDQPISIYEVHLGSWRRHTDNNFWLSYRELADQLIPYVKEMGFTHLELLPVNEHPFDGSWGYQPQGLYAPTRRFGTRDDFLYLIGAAHEAGLNVLLDWVPGHFPTDDAGLAKFDGTALYEHGDPREGYHQDWNTLIYNYGRREVSNYLVGNALYWIERFGIDGLRVDAVASMIYRDYSRESGKWVPNEYGGRENLEAIEFLRSTNRIIGEQTPGAVTMAEESTDFAGVSRPPSMGGLGFWFKWNLGWMHDTLDYMKLDPVYRQHHHDKMTFGMLYNGTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLLFMGNEFAQGREWNHDSSLDWHLLQGGDNWHHGVQRLVRDLNHTYRRHAALHQLDFDGYGFEWLVVEDKANSVFVFVRRDAEGNEVIVASNFTPVPRYHYRFGVNQPGRWREELNTDSMHYHGSNAGNSGTVRSEAVPSHGRDHSLCITLPPLSTLCPAPFGAYFDGKGVNFTLYSVHAEKVELCLFDEHGHERRYDMPSRSGDIWHGYLPDGKPGLLYGYRVHGPWEP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have cation binding, SHG alpha-glucan phosphorylase activity, isoamylase activity within cytoplasm to regulate glycogen catabolic process, cellular nitrogen compound biosynthetic process, glycogen biosynthetic process.2. We need a protein that can perform [(1->4)-alpha-D-glucosyl](n) + phosphate = [(1->4)-alpha-D-   glucosyl](n-1) + alpha-D-glucose 1-phosphate with high efficiency.3. For general function, the protein need meet that Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.4. The protein design should take into account the physiological conditions of the glycogen biosynthesis, Glycan biosynthesis pathway.5. The designed protein should contain one or more Glycosyl hydrolase family 13 catalytic domains that are essential for its function. The designed protein sequence is ",
    "response#1": "AMLLAGGKGSRLSGLTKNMAKPAVSFGGKYRIIDFTLSNCSNSGIDTVGILTQYQPLELNSYIGIGSAWDLDRYNGGVTVLPPYAESSEVKWYKGTASSIYENLNYLNQYDPEYVLILSGDHIYKMDYGKMLDYHIEKKADVTISVIEVGWEEASRFGIMKANPDGTITHFDEKPKFPKSNLASMGIYIFNWPLLKQYLEMDDQNPYSSHDFGKDIIPLLLEEKKKLSAYPFKGYWKDVGTVQSLWEANMDLLEVHAGSWQRSEDGTFLSWDELEAQLIPYCTDMGFTHIEFLPITEHPYDPSWGYQTTGLYAPTARFGDPEGFARFVNGAHKVGIGVLLDWVPAHFPTDEHGLRWFDGTALYEHADPRQGFHPDWNTAIYNFGRIEVMSYLINNALYWAEKFHLDGLRVDAVASMLYLDYSRKEGEWIPNEYGGRENLESVRFLQKMNSLVYGTHPGVMTIAEESTSWPKVSQPVHEGGLGFGFKWNMGFMHDTLSYFSREPVHRKFHHQELTFGLLYAFTENFVLPLSHDEVVHGKGSLIAKMSGDDWQKFANLRSYYGFMWGYPGKKLLFMGQEFAQWSEWSEKGSLDWNLRQYPMHEGMRRLVRDLNLTYRSKAALHARRPAVHSRDWYEGTADAWIDANDSANNVLSFLRHGSDGSVMACIFNFAGSEHSDYRLGLPIAGRWREVLNTDATIYNGSGVGNFGGVDATAEPWHGRPASAVLVLPPSSALWLEL",
    "response#2": "AMLLAGGKGSRLSGLTKNMAKPAVSFGGKYRIIDFTLSNCSNSGIDTVGILTQYQPLELNSYIGIGSAWDLDRYNGGVTVLPPYAESSEVKWYKGTASSIYENLNYLNQYDPEYVLILSGDHIYKMDYGKMLDYHIEKKADVTISVIEVGWEEASRFGIMKANPDGTITHFDEKPKFPKSNLASMGIYIFNWPLLKQYLEMDDQNPYSSHDFGKDIIPLLLEEKKKLSAYPFKGYWKDVGTVQSLWEANMDLLECHVGSWARRDDGWFLNYRDLAHRLGEYVTYMGYTHVELLGVMEHPFDGSWGYQVTGYYAPTSRLGSPEDFKYLVNHLHSLGIGVLLDWVPGHFPTDEFALAHFDGSPLYEYADPRKGYHYDWNTYIFDYGRNEVVMFLIGSALKWVQDYHVDGLRVDAVASMLYLDFSRTEWVPNIYGGRENLEAIAFLKRLNEVAHHMAPGCLMIAEESTSFPGVTTPTPEGLGFDYKWAMGWMNDSLAYFEQDPIYRKYDHHKLTFFNVYRTSENYVLAISHDEVVHLKKPMVLKHPGDWYAQRADYRAFLAMMWTTPGKKLLFMGQDFAQGSEWNHDAPLPWFHADQPDHRGVMNLVECHVGSWARRDDGWFLNYRDLAHRLGEYVTYMGYTHVELLGVMEHPFDGSWGYQVTGYYAPTSRLGSPEDFKYLVNHLHSLGIGVLLDWVPGHFPTDEFALAHFDGSPLYEYADPRKGYHYDWNTYIFDYGRNEVVMFLIGSALKWVQDYHVDGLRVDAVASMLYLDFSRTEWVPNIYGGRENLEAIAFLKRLNEVAHHMAPGCLMIAEESTSFPGVTTPTPEGLGFDYKWAMGWMNDSLAYFEQDPIYRKYDHHKLTFFNVYRTSENYVLAISHDEVVHLKKPMVLKHPGDWYAQRADYRAFLAMMWTTPGKKLLFMGQDFAQGSEWNHDAPLPWFHADQPDHRGVMNLV",
    "response#3": "AMVLAGGKGERLAPLTLRRPKPGVAFGGKYKIIDFVLSNMFNSGIKKVYILTQYRAYSLMKHIRESWGKWAGLGEFFVAISPETSSESEEWFKGTADAINHYLRFIESSDADYVAIFGGDHIYRMDVSQMIGYHRRNRADITIAALEVPVEEARRFGVFCVDDDNRVTAFEEKPANPVTIPGRETCFASMGNYIFSTRRLIEVLQEGKKLHADLDFGKHVIPMMLAKKDRVFAYNFNDNLIPGMKPEERGYWKDVGTIDSYYEANMEEVHLGSWRPGLSYRQLATELTDYVVAHKFTHVELLPVAEHPFAGSWGYQVTSYYAPTSRFGTPDDFRALVDALHQAGIGVIVDWVPAHFPKDAWALGRFDGTPLYEHSDPKRGEQLDWGTYVFDFGRPEVRNFLVANALYWIEQFHIDGLRVDAVASMLYLDYSRPQDGWTPNIYEGRENLEAVQFLQEMNATAHKAAPGIVTIAEESTSWPGVTRPTSIGGLGFSMKWNMGWMHDTLDYVSRDPIYRSYHHHEMTFSMLYAFSENYVLPLSHDEVVHGKGTLWGRMPGSNHTKAAGLRSLLAYQWAHPGKQLLFMGQEFGQRAEWSEQRGLDWFQLDENGFSNGVLRLVRDLNHIYQGSGLWELAYNLWWTWNPPAKELFRSIDPLLWKETKENPIELLRKTKLLENKLKDEDFISHFKYVYSLYKTYMNRHSKYEDTYKKPIVFLSPEYGLHHTWLVIDDRTQSVFAYMRYGADSDAPVLVVCNLTPVPRHDYRIGVPQGGYWREILNTDAGIYGGSNTGNGGGRMADATPSHHHPASLQLTLPPLATIILKP"
  },
  {
    "reference": "MGGKWSKSSXXXWPEVREXIXQTPAAAEGVGAVSQDLAKHGAITSSNTAANNPDCAWLEAQEEDSEVGFPVXPQVPLRPMTXKAAFDLSFFLKEKGGLDGLVHSKKRQEILDLWVYXTQGFFPDWQNYTPGPGTRXPLTFGWCFKLVPXDPAEVEENNKGENNSLLHPICQHGMEDEDREVLVWRFDSSLARRHLAREMHPEXYKDC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein's functional design necessitates the inclusion of a Dileucine internalization motif; necessary for CD4 internalization, Diacidic; necessary for CD4 internalization motif.2. The protein must exhibit the following characteristics: Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1.3. The protein should be optimized for stability and expression in extracellular region, host cell Golgi membrane, membrane, virion component where it will participate in suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II. The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPAVRERIRRAXPAAEGVGAASQDLDKYGALTSSNTAETNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAXDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGXRYPLTFGWCFKLVPVDPDKVEKANEGENNSLLHPMSLHGMEDPEGEVLQWKFDSRLAFHHVAREMHPEYYKNC",
    "response#2": "MGGKWSKRSGAGWPAIRERMRRXEPAAEGVGAASXDLDKHGALTTSNTAANNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKXAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVDPREVEEANEGENNCLLHPMSQHGMEDEHREVLKWKFDSQLARRHMARELHPEYYKDC",
    "response#3": "MGGKWSKRSGAGWPAVRERMRRAEPAAEPAAEGVGAASRDLAKHGAITSSNTAATNADCAWLEAQEDEEVGFPVKPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQRRQDILDLWIYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPEKVEEVTEGENNSLLHPICQHGMDDEHKEVLMWKFDSRLAFHHTARELHPEYYKN"
  },
  {
    "reference": "MDQSFVTERLEATCRTFNALERQLADPSVAADPEQLLTLAKERSRLEPLVLDYQRLQQLHAEHQQAQQLLKESKGDAELEALAQEELQQLSSEQEQLNQRLKVALLPSDPRDERSVMLEIRAGAGGDEACLWAGDLARMYERHAQTCGWQVNPVSASEAELGGFKELILAIRGDAVFSQLKYEAGVHRVQRVPATESQGRVHTSTATVAVMPEADPVDVQIDPKDLDISTARSGGAGGQNVNKVETAVDLLHKPTGIRVFCTQERSQLQNRERAMEILRAKLLAKEEEEAAAAESSARRAQVGSGDRSEKIRTYNYKDNRTTDHRLGKNFPLETVLNGQLSDLIEACTHADQQQKLEELAASES",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.2. The protein must exhibit the following characteristics: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.. The designed protein sequence is ",
    "response#1": "RQRLEELETVIAEPDFFNDQKKASEIMREHRRLKELMETWDSLNATEQQLADNQELAKTDDPELAELAALEIPELEAALEKLRSDVQYSLLPRDTTEDRDAIIEIRAGTGGDEASLFAGDLLRMYQRFAEERGWRFEHLESSPSDVGGFKEVVCRIAGEEVFRFLKYEGGVHRVQRVPATETQGRIHTSTATY",
    "response#2": "ELASMEEDEETLNEVFNDVQDLKKIIRDLEIEVMLSDENDAKNAIISIHPGAGGTESHDWASILYRMYLRYAERRGWKVEVLDYQAGDEAGIKDVSFLVKGENAYGYLKAI",
    "response#3": "QEKYLRIEDELAKATASDTLKNLSKERSRLTPVYTKADEYLKITKDCQDAKSLLESENDPDMHSMLKSEIEEGEKKLEELAKELEIMLLPPDPNSGKSILVEIRAGTGGEESGLFCADLFRMYNKYADKKGLRVEIIDMSQTGIGGYKEIVFSLDDDKAYDLFKFESGTHRVQRIPETESGGRIHTSAVTS"
  },
  {
    "reference": "MVRAAFIGWRGMVGSVLMDRMITEKDFTGFEPVFFTTSQVGQLGPDIGLDIPPLKDAYDITQLSSADIIVTCQGSDHTKEVYPKLRQGGWKGFWIDASSALRMKDDSIIVLDPVNRDVIDKAISSGIKDYIGGNCTVSLMLMAIGGLFHKGLVEWITSMTYQAASGAGAKNMRELIEQMACIGTASKDLLDNPASAALDLDKRVIETMRGSDFPVANFGFPLAGSVLPWIDSELENGQSREEWKGIAETNKILGTKKTIPIDGLCVRIGAMRCHCQALTIKLTRDVPLDEISGIISQANDWVKVIPNTKEQTLKHLTPAAVTGRLDVPVGRIRKLNISPEYLTLYTVGDQLLWGAAEPIRRMLKIALEHVS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should take into account the physiological conditions of the L-threonine biosynthesis pathway.2. The protein must exhibit aspartate-semialdehyde dehydrogenase activity. The designed protein sequence is ",
    "response#1": "PLHAAAGLKAMTATSYQSVSGSGQTGMDVLAHEIDVLGKDRELLAKGGWSEPGSDIYSRPIGFNVLPHAGNFTEQGYTDEEWKLVNETRKILEAPDVLVEPTCVRVPVMVGHGISATMHFDRPVSLNEANEVLAAAPGAQLWDDSKVPTPLDSAGIDDILIGRVRPTIGEPGGISLWTVGDNLRR",
    "response#2": "PLHAAAGLKAMTATSYQSVSGSGQTGMDVLAHEIDVLGKDRELLAKGGWSEPGSDIYSRPIGFNVLPHAGNFTEQGYTDEEWKLVNETRKILEAPDVLVEPTCVRVPVMVGHGISATMHFDRPVSLNEANEVLAAAPGAQLWDDSKVPTPLDSAGIDDILIGRVRPTIGEPGGISLWTVGDNLRG",
    "response#3": "PLHAAAGLKAMTATSYQSVSGSGQTGMDVLAHEIDVLGKDRELLAKGGWSEPGSDIYSRPIGFNVLPHAGNFTEQGYTDEEWKLVNETRKILEAPDVLVEPTCVRVPVMVGHGISATMHFDRPVSLNEANEVLAAAPGAQLWDDSKVPTPLDSAGIDDILIGRVRPTIGEPGGISLWTVGDNLRH"
  },
  {
    "reference": "MNANPPLRVLLGVTGGIAAYKSAELVRRLRERGAEVQVVMTDGARQFVTPLTFQALSGRTVRSDLWDEAAEAAMGHIELARWADRVVVAPASADFLARLAHGFAGDLLSTVCLATEAPIAVAPAMNRLMWANPATQANVAMLAGRGVTILGPASGDQACGETGPGRMLEAAQIAEALLAPCADAGGALRGRKVLITAGPTRERIDPVRFITNRSSGKMGYAVAEAARAAGAEVVIVSGPVAVPTPAGTRRIDVETAEQMMDVVAAELPGTDIFIAAAAVSDYRPVQPATEKIKKTSDSLVLALTRTTDVLATVAAGSPRPFVVGFAAETQNVERNALAKLEGKRLDMIAANEVGDRLAFDCDDNALTVYWPGGRRELGRAPKRELADALVALIAERYGAGRASGAEPGASRGAGSIAAR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be designed to catalyze the following reaction H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.2. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A. The designed protein sequence is ",
    "response#1": "RVLLGVAGGIASYKTAWLARLLSKAGAEVDVVMTRAATEFVGAVTFEALTGRPVHTGLFDPGRALDHIQLARAAHAVVVAPATADFMARAAAGQADDLLTACLLATQAPVLLVPAMNDRMWAHKQTQANAVHLRQLGYRLLDPDEGMLAAGEGSGPGRMPEPETILAHIRTAVAAGA",
    "response#2": "EDRRVVITGGGTREKIDEVRYISNFSSGKMAKAIALSLYLKGADVCYITTKGNDSLPKSIYTIEVDDAQEMLEYTQDALRIAKKGKLSKASLTTADAIKLIQKRPFLFMVAAVADFTPKFPQQGKLKKSTLGENWQIELTQTSDILSTLAKDREGVTTIAFKAEMDTQEGLNNAKELLTKKEVDAVCYNLLEDAKSFGGDQNSITFVE",
    "response#3": "KVLVGLTGGIACYKVPYLIRDLIREGAEVRVVMTAAATKFITPLTMETVSANPVAIEMFPGDRFVGTQHIDLAKWPDLVVVAPATANFLGKVASGICDDLLTTIICATPSPVIVAPAMNPQMWKHPSTQRNAAYLGEIGYQFVGPAEGEMACNESGLGRMVEPSELLAGERVLVTSGPTREPIDPVRYLSNRSSGKMGHAVASAALRRGAEVVLISGPTTLQPPPGAVYVPVQTAEEMREASLQHLASASVVIKAAAVADYRVERPSATKIKSGGKDEGLTLSLVANPDILKELAQRKGGAFLVGFAAETNDVRKHAADKLRAKGVDLLVVNDVSQQGIGFDAEDNQVLLLWPDGRIEPLPRADKQVLARQLVALIALRLR"
  },
  {
    "reference": "MSRGEEVRRMFDAIAWRYDLMNRLMTLGQDQRWRRFVVKQSGLEDHGAMLDLACGTGDITAAAHHSHPHCTIVGADFSPIMLHVAKKRFSHLPIHWQMADAYQLPFRSGSLSAVTFGYLMRNVDEPVAVLREVHRVLAPGGRVVCLDTTPPRGLMRPLTNFHFRVIIPLLGRLVAGDHGAYQYLTGSTMGFYEADQLAGLFTEAGFSVVGFRKFMLGTIAVHWGSR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be able to bind S-adenosyl-L-methionine ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "VGAGPGDPDLLTFRALRLMQQADVVLHDRLVPDAIIDLCRRDAERIYVGKARANHAVPQEQINQLLVRLAKEGKRVLRLKGGDPFIFGRGGEEIQELTANAVPFQVVPGITAASGCSTYAGIPLTHRDFAQSVRFITGHLKDGSTNLPWADLVAPGQTLVFYMGLVGLPEICARLIEHGRGGETPMALIQQGTTINQKVLIGTLATMPELVRNTEIRPPTLLIVGEVS",
    "response#2": "GEVYLVGAGPGDPDLLTFRALRLMQQADVVLHDRLVPDAIIDLCRRDAERIYVGKARANHAVPQEQINQLLVRLAKEGKRVLRLKGGDPFIFGRGGEEIQELTANAVPFQVVPGITAASGCSTYAGIPLTHRDFAQSVRFITGHLKDGSTNLPWADLVAPGQTLVFYMGLVGLPEICARLIEHGRGGETPMALIQQGTTINQKVLIGTLATMPELVRNTEIRPPTLLIVGEVVSLHG",
    "response#3": "GEVYLVGAGPGDPDLLTFRALRLMQQADVVLHDRLVPDAIIDLCRRDAERIYVGKARANHAVPQEQINQLLVRLAKEGKRVLRLKGGDPFIFGRGGEEIQELTANAVPFQVVPGITAASGCSTYAGIPLTHRDFAQSVRFITGHLKDGSTNLPWADLVAPGQTLVFYMGLVGLPEICARLIEHGRGGETPMALIQQGTTINQKVLIGTLATMPELVRNTEIRPPTLLIVGEVVSLAP"
  },
  {
    "reference": "MTALGVTVALLVWVTALLCTFLWKQVYSIRKLPPGPFPLPIVGNLFQLELSDVPKSFTRLAERLGQVFTLHLGSNRVVVLHGYQHKKEFSGRGEYHTYRAHKDQGTTFNNSPTRRETQRASLTILRDFGMGKEGNEARIQREIPFVVEALRETQGQPFDPTLVVGCAACNVIADILFRKRFDYGDRTCLRLQTLLNENLYLVSTPWLLLQNNFASLFNYLPGSHRKAMKNVSELKDYALARLKEHQESLAPSCPRDFSDRLLMEMEKEKYKAEPGYTLGNIASTVADLFFAGTVTTSATLRYGLLVLLKHPEVEEKLHEEIDRVIGPHRIPSIKDKLEMPYMDAVVHEIQRFINFLPSNMNHEATEDTVFRGYIIPKGAILIPTLDSLLFDNQEFPDPDKFKPEHFLDEKGNFKYSDYFKALSAGRRVCVGEGLAHMELFLFFTAILQHFNLKSLIDPKDIDLTPIVNGFASIPPNYKLCLLPRSHA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that can modulate the overall flux of metabolites through the fatty acid metabolism pathway, enhancing or suppressing its activity as needed.2. The designed protein should be involved in fatty acid metabolic process by localizing to mitochondrial inner membrane, endoplasmic reticulum membrane and exhibiting aromatase activity, Hsp90 protein binding, heme binding, Hsp70 protein binding.3. The protein must exhibit the following characteristics: Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids; Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase); May be involved in the oxidative metabolism of xenobiotics.; A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. The designed protein sequence is ",
    "response#1": "MGWSSVLFVLVCLTGVEGKVTLVESGGGLVQPGGSLRLSCAASGFTFSDYAMSWVRQAPGKGLEWVAYISSGSTRYYSPAVQGRFTISRDNSQSMVYLQMNSLRPEDTAVYYCARDTVRGAQCEAQHSPLRGQGTRLTVREP",
    "response#2": "MRVLLLVTLLVASVHCAPVADESNKVPAELRSMWNEYKTAYMKSYDSATEEENRFKIFRDNVEKIQAYNLQAAKGLHTYTLGMNVFSDLTSEEFNAMMNGFKLPETSRRVNTTFRSPNLPETMDWRNKGYVTPVKNQNPCGSCWAFSATGALEGQHFRKTQQLVSLSEQNLVDCSGAYGNHGCNGGWMTSAYQYVKNAGGIDSEASYPYVGHQGKCRYNPRKATVADTGFVDIPSGDEKALKEAVATVGPVSVAIDASHLSFQLYKSGIYDEPECSENQLDHGVLAVGYGTESGQDYWIVKNSWGTTWGDGGYIKMARNKNNMCGIATAASYPLV",
    "response#3": "MAGGSVEFSAGKRVCVFL"
  },
  {
    "reference": "MSDEEQSAIVCDNGSGMVKAGFSGDDALCHVFPSIVGRPKNEQAMMGSASKKLFVGDEAQAKRGVLSLKYPIEHGIVTNWDDMEKIWHHTFYNDVRVNPESHSVLLTEAPMNPKQNREKMTQIMFETFGVPAMYVGIQAVLSLYSSGRTTGIVLDAGDGVTHTVPIYEGYSLPHAIRRMDMAGRDLTEYLMKLLMESGMTFTTSAEKEIVRNVKEQLCYVALDFDEEVTNSAKTVNEEPFELPDGTIMQVGNQRFRCPEALFKPMLIGLDEAPGFHEMTFQSINKCDIDVRRDLYGNIVLSGGTTMFKNLPERLGKEISNLAPSSIKPKVVAPPERKYSVWIGGSILSSLTTFQTMWIKKSEYDEAGPSIVHNKCF",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that localizes to membrane-bounded organelle, cytoplasm and can perform ATP binding with high specificity and efficiency.2. The protein must exhibit the following characteristics: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.. The designed protein sequence is ",
    "response#1": "MGQ",
    "response#2": "GWISKAEYDL",
    "response#3": "GWISKAEYDL"
  },
  {
    "reference": "MLDQQTIATIKSTIPLLESAGPAMTKHFYQRMFTHNPELTEVFNLAHQHSGGQPVALFNAIAAYAQHIDNLAALTGAVERIAHKHTGFMIQPQQYHIVGTHLLATLKELGGEAVTDGILAAWEKAYGLLASVFIGREEQIYTEREQAAGGWRGTRPFIIRQKRVESDLITSFVLEPKDGGQVVDFIPGQYLSLQLSHPALAHTEIRQYSLSDAPNGRHYRISVKREAGGQVSNLLHDHFEEGDELAVVPPAGDFVLQAEPDTPVVLLSGGVGLTPMLSMLGHLLTSGHQAEIRWLHACEHGGLHAFADEIATKAASHPNLEARTWYRQPRDEDKGRFDFSGQMDLEQIYELLPAGAHYYFCGPEGFMREVKRQLSERGVEEGRLHYEFFGPHQSL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. heme b is required for the proper folding and stability of the protein.2. The designed protein must have at least one Globin family profile domain.3. The designed protein should be able to compete with endogenous proteins or inhibitors for heme b binding.4. The protein must exhibit metal ion binding, oxygen carrier activity. The designed protein sequence is ",
    "response#1": "VKKTWSTVKFNEVDILYAVFKAYPDIMAKFPQFAGKDLDSIKDSAAFATHATRIVSFLSEVISLAGSDANIPAIQNLAKELATSHKPRGVSKDQFTEFRTALFTYLKAHINFDGPTETAWTLALL",
    "response#2": "VKKTWSTVKFNEVDILYAVFKAYPDIMAKFPQFAGKDLDSIKDSAAFATHATRIVSFLSEVISLAGSDANIPAIQNLAKELATSHKPRGVSKDQFTEFRTALFTYLKAHINFDGPTETAWTLALL",
    "response#3": "VKKTWSTVKFNEVDILYAVFKAYPDIMAKFPQFAGKDLDSIKDSAAFATHATRIVSFLSEVISLAGSDANIPAIQNLAKELATSHKPRGVSKDQFTEFRTALFTYLKAHINFDGPTETAWTLALM"
  },
  {
    "reference": "MKLLGRKKSYGQDIETSDDNVGSEASLPDTLSRGSSTTAPKGRPTRKRDDADRRHTKKGPITPAPMTASEARARRKSLAPPKCHRAERRAKRAASKAQITDRRERMMAGEEAYLPPRDQGPVRRYIRDLVDARRNALGLFTPSALVLLFITFGVPQLQLYMSPAMLVLLSVMGIDGIILGRKISKLVDVKFPSNTESHWRLGLYAAGRASQMRRLRVPRPQVEHGSSVG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have a specific function within the plasma membrane.2. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "IMSTIFLSTLLAALDIGIVATAIPGITDDFHRLDDVGWYGGACFLLVGSSAPMWGKLYKYFSAQLVYLVSVVIFLVGSIVAAAAPNSAALIVGRALQGWGCSGTLGGSVLMISYVAEPQKRAMLIGMWMSVFMFSTIIGPLLGGAFTSEVTWRWCFWINLPVGGPVIALVVLFFEVPKHIKPAPATWVEILRQLDLPGFALLLTSLVCLTVALQWGGQTKSWSYGSVIATLVMWVVLTIAFFVVEWIQGDYAMVPLALLKPRLVWTNALYGWIANLANFQVLFYLPIYFQSIHGQSAIASGVNSLPFMAFFAAGSMLSGFLIGKTRLLQPYEFASGVLATVGAALLYTLDIDSSKARYIGPQVIFGIGIGLGNQVPMTALESFSKPEHIAPTTGVMLMCNSISGAYFVTAAQSIFANYMLKELASIAPDMDPIQVLTTGASEVSHVFQGAELEAVLAAYLAGIKDVFAFSLAGAAFTVVLSLAIPFKKNQ",
    "response#2": "IMSTIFLSTLLAALDIGIVATAIPGITDDFHRLDDVGWYGGACFLLVGSSAPMWGKLYKYFSAQLVYLVSVVIFLVGSIVAAAAPNSAALIVGRALQGWGCSGTLGGSVLMISYVAEPQKRAMLIGMWMSVFMFSTIIGPLLGGAFTSEVTWRWCFWINLPVGGPVIALVVLFFEVPKHIKPAPATWVEILRQLDLPGFALLLTSLVCLTVALQWGGQTKSWSYGSVIATLVMWVVLTIAFFVVEWIQGDYAMVPLALLKPRLVWTNALYGWIANLANFQVLFYLPIYFQSIHGQSAIASGVNSLPFMAFFAAGSMLSGFLIGKTRLLQPYEFASGVLATVGAALLYTLDIDSSKARYIGPQVIFGIGIGLGNQVPMTALESFSKPEHIAPTTGVMLMCNSISGAYFVTAAQSIFANYMLKELASIAPDMDPIQVLTTGASEVSHVFQGAELEAVLAAYLAGIKDVFAFSLAGAAFTVVLSLAIPFKKTR",
    "response#3": "IMSTIFLSTLLAALDIGIVATAIPGITDDFHRLDDVGWYGGACFLLVGSSAPMWGKLYKYFSAQLVYLVSVVIFLVGSIVAAAAPNSAALIVGRALQGWGCSGTLGGSVLMISYVAEPQKRAMLIGMWMSVFMFSTIIGPLLGGAFTSEVTWRWCFWINLPVGGPVIALVVLFFEVPKHIKPAPATWVEILRQLDLPGFALLLTSLVCLTVALQWGGQTKSWSYGSVIATLVMWVVLTIAFFVVEWIQGDYAMVPLALLKPRLVWTNALYGWIANLANFQVLFYLPIYFQSIHGQSAIASGVNSLPFMAFFAAGSMLSGFLIGKTRLLQPYEFASGVLATVGAALLYTLDIDSSKARYIGPQVIFGIGIGLGNQVPMTALESFSKPEHIAPTTGVMLMCNSISGAYFVTAAQSIFANYMLKELASIAPDMDPIQVLTTGASEVSHVFQGAELEAVLAAYLAGIKDVFAFSLAGAAFTVVLSLAIPFKKTS"
  },
  {
    "reference": "MAETGSKRILLIVGGGIAAYKSCELVRLIRKGGAEVTCVLTDGGAQFVTPMTLAALSENPVHTTLWDLKNEAEMGHIQLSREADLVVVCPATADLMAKMAAGVADGLATTLLLATDKPVLAVPAMNVKMWEHPATQRNVARLREDGIRVMDPDEGPMACGEFGAGRLPEPEAIWAAIRDFGVAPDPLAGQPDFAGEAHRRLFGKHVLITAGPTHEPIDPVRYIANRSSGKQGFAIAAAAAEAGARVSLIAGPVHLDTPPGVERVDVESAREMMAEVEKALPADVAIMVAAVADWRAADTAGQKIKKDGSGAVPPLALMENPDILATLAQSPQRPGLLIGFAAETQDIVEHATAKRTRKGADWIVANDVSGPKGQSVMGGDTNTVQIVTASGTESLPAMPKGQVAAAIIDRIANEIA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.2. The protein design must be able to perform phosphopantothenate--cysteine ligase activity, FMN binding, phosphopantothenoylcysteine decarboxylase activity, metal ion binding in order to contribute to pantothenate catabolic process, coenzyme A biosynthetic process.3. The protein with the reaction H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2, the Proton donor must be optimized to ensure efficient catalysis.4. The designed protein must have at least one Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domain.5. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway to avoid interference with native metabolic processes.6. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.7. The designed protein should have a binding site that is specific to the target CTP ligand. The designed protein sequence is ",
    "response#1": "MRIVLGVTGGIAAYKAALVLRLLKEAGHRVRVVPTESALRMVGRPTWEALSGEPVRTSVFDDAEHVDHVALGRGADLVIVAPATANLLAKAATGLADDLLSTTLLTVTCPVLLAPAMHTEMWLHPATQANVATLRGRGVHVLDPDSGRLTGADSGPGRLPEPAAIVAAALAVLDGPAGDLLAGRRVLVTAGPTHEPIDPVRYLANRSSGKQGFALAGALARVGAEVVLVAGPCALATPAGVTRVDVETAREMLSACRDALPVDAAICVAAVADWRVAETGAQKLKKDATGGAPPALALTENPDILATLAQLETGRPALVVGFAAETDRVLDHARAKRARKRCDWIVANDVSAATGIMGGDDTAVTVVAADGTVTELPLGSKHDLADAVWDAVVAAL",
    "response#2": "MRIVLGVTGGIAAYKAALVLRLLKEAGHRVRVVPTESALRMVGRPTWEALSGEPVRTSVFDDAEHVDHVALGRGADLVIVAPATANLLAKAATGLADDLLSTTLLTVTCPVLLAPAMHTEMWLHPATQANVATLRGRGVHVLDPDSGRLTGADSGPGRLPEPAAIVAAALAAVGDVRPSGLAGRRVLVTAGPTHEPIDPVRYLANRSSGKQGFALAGALARVGAEVVLVAGPCALATPAGVTRVDVETAREMLSACRDALPVDAAICVAAVADWRVAETGAQKLKKDATGGAPPALALTENPDILATLAQLETGRPALVVGFAAETDRVLDHARAKRARKRCDWIVANDVSAATGIMGGDDTAVTVEVLTADGATEWLRRDKSELAGRLWELVASRL",
    "response#3": "MRIVLGVTGGIAAYKAALVLRLLKEAGHRVRVVPTESALRMVGRPTWEALSGEPVRTSVFDDAEHVDHVALGRGADLVIVAPATANLLAKAATGLADDLLSTTLLTVTCPVLLAPAMHTEMWLHPATQANVATLRGRGVHVLDPDSGRLTGADSGPGRLPEPAAIVAAALALLEPKSLAGRRVLVTAGPTHEPIDPVRYLANRSSGKQGFALAGALARVGAEVVLVAGPCALATPAGVTRVDVETAREMLSACRDALPVDAAICVAAVADWRVAETGAQKLKKDATGGAPPALALTENPDILATLAQLETGRPALVVGFAAETDRVLDHARAKRARKRCDWIVANDVSAATGIMGGDDTAVTVELLGADGSRRELPRADKRVLARKLWDLVAERLPR"
  },
  {
    "reference": "AKEKIHINIVVIGHV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis.2. The protein must exhibit GTP binding. The designed protein sequence is ",
    "response#1": "KPHVNIGTIGHVDHGKTTLTAAITMTLAALGNAQAKKYDEIDAAPEEKARGITINTAHVEYETASRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLARQVGVPNLVVFLNKKDMVDDEELLELVELEVRELLTNYDFAGDDIPIIAGSAKEALEYMTKNPKAQKGDNEWVDAIYELMEAVDSYIPS",
    "response#2": "IMEPIMIVSVTSPNEFQGNVIGLLNKLQAVIQETDNGHDEFTLRAECSLSTMFGFASSLRASTQGKGEFSLEFSHYAPTAPHVQKR",
    "response#3": "PHVNIGTIGHVDHGKTTLTAAITMTLAALGQAVAKGYDQIDNAPEEKARGITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLAKQVGVPKLVVFLNKEDMMEDAELLELVELELRELLTEYEFDGDDIPIVRGSGLQALEVMTKNPKTQRGENPWVDKIYELMDAVDSYIPDR"
  },
  {
    "reference": "MIYGKNILLCVTGGIAVYKAAALTSQLTQRGANVKVIMSEAACKFVTPLTFQTLSRNDVYIDTFEEKQSAVIAHIDLADWADLVVVAPATANTIGKLAHGIADNMVTTTLLATTAPVWVAPAMNVHMYEHPAVRENIEKLVRFGYRFIEPSEGHLACGYVGKGRLEEPENIIVAIEQYFSSPSPLLKGKKVLVTAGPTREKIDPVRFFTNRSTGKMGYAIAETAAEFGAEVTLVSGPTNLPSPPNVETIHIESAQEMYEEVIKRFPHVDIVIKSAAVADYRPKHVFHEKMKKQPGDLIIEMERTIDILKTLGEQKTTQFLVGFAAETEHVEEYARKKLQSKNLDMIVANNVSQEGAGFAGDTNIVTIFKRNGSVMTLPLLPKKKVAEEILKEIVGAMK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that is capable of catalyzing reactions within the coenzyme A biosynthesis pathway with high efficiency and specificity.2. The designed protein should contain one or more DNA/pantothenate metabolism flavoprotein C-terminal domains that are essential for its function.3. The designed protein must be able to perform phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity under specific conditions.4. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine. The designed protein sequence is ",
    "response#1": "MKIALGVSGGIAAYKAAEIVRLLQDRGIRVQVVMTAAAQEFVRPLTFAALSGEKVITSMFGSGGDEQANVESAIEHIAIAQAIDALVVAPATADVLARFAQGIANDFLSTLYLATTAPVVVAPAMNVNMWNHVATQANLEILRKRGVRIVEPGSGYLACGMTGAGRLAEDETIVAAVMEHAERVLAGRLRGRKIVVTAGATRERIDPVRFISNHSSGKMGVALAQAAWRRGADVVLIAGHVDVAVPPEIGATTIETVDEMAKAVGDELPSADVLIMAAAPADFRPAEQVTQKIKKGETAPRIALEPTLDILKSTIPKRKKKSVIVGFALETNDGAKNAREKLESKQLDFVVLNNALEPGAGFGVDTNRVTILFADGSAADLPVMSKHDLADAVWDLVAARLVPPG",
    "response#2": "RIVLGLSGGIACYKSAELVRLLTKAGASVQVVMTEAAQQFITPVTMQALSGHPVYTDQWDAREPNNMAHINLTRGADAIVIAPASADCIARLAHGRADDLLSLTCLARPWGAAEGRCPLLVAPAMNREMWAHPATQRNVAQVIADGAIVLGPDRGDQACGEVGDGRMLEPADLFEEIVTHFQKQANQHDLLHGLHVVVSAGPTREYLDPVRFISNPSSGKMGFAVAARARARGARVTLVSGPVNLEVPPGVQFVPVESALEMRDAMLAASESADMVVMTAAVADYRAARPAGEKQAKGSEALTVELTPNPDILAELGSNKGERVLVGFAMETHAGVERAAHKARRKNADFILLNYPTREGTGFGGDDNEVTLITADGVEQLPLAPKRQVAEQLVQLIAERLNAE",
    "response#3": "KVVLGVTGGIAAYKTAELARIFIRSGVEVQVVMTEAASRFVNPLTFTTLTGNPVHFQMYDGQSTGIRHIDLSEGADAMIIAPATANTIAKMATGLADNLLTTLFLATLCPVIIVPSMNVKMFEHPAVQNNLQVLRSRGCHILEPNAGELACGEYGRGRMPEPQEIFDLVVLAERMLAGGLKGRRILVTSGPTHEPIDPVRYIANRSSGAQGTAIAKALADLGGEVVFVTGPADVPPPEGVQVIRVETALQMQAAVEAALPVDAGVFAAAVADWRVSSASDRKLKKSKDGLPALEFAENPDILKAVSHLKEDRPKLVVGFAAETNDVVENATAKRVRKGCDWIVANDVSPATGIMGGSENAVVILVSADATEELPYGPKEALADRLWELVAERL"
  },
  {
    "reference": "MLIYKDIFTDDELSSDSFPMKLVDDLVYEFKGKHVVRKEGEIVLAGSNPSAEEGAEDDGSDEHVERGIDIVLNHKLVEMNCYEDASMFKAYIKKFMKNVIDHMEKNNRDKADVDAFKKKIQGWVVSLLAKDRFKNLAFFIGERAAEGAENGQVAIIEYRDVDGTEVPTLMLVKEAIIEEKC",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must be able to calcium ion binding. The designed protein sequence is ",
    "response#1": "VRIGLVGKYVNLPDAYLSVAEALRHGGYDCGARVDIDWIASDDAEGLLAEGRLHELDGIVVPGGFGFRGIEGKIAAVGYARENDIPFLGLCLGMQCAVVEFARNVCDLAGANSSEFDPHTPHPVIDLMDEQRDVIDMGGTMRLGIYPARLEPGSIVSAAYGEEVIYERHRHRYEVSNKHRPRLEEMGLVCSGTSPDGRLVEFVELPTDKHPFFVATQAHPEFKSRPDRPHPLFAAFVRVTQ",
    "response#2": "AVWQFRNMIKCTIPGSDPLREYNNYGCYCGLGGSGTPVDDLDRCCQTHDHCYNQAKKLESCKFLIDNPYTNTYSYKCSGNVITCSDKNNDCESFICNCDRQAAICFSKVPYNKEYKDLDTKKHS",
    "response#3": "VRIGLVGKYVNLPDAYLSVAEALRHGGYDCGARVDIDWIASDDAEGLLAEGRLHELDGIVVPGGFGFRGIEGKIAAVGYARENDIPFLGLCLGMQCAVVEFARNVCDLAGANSSEFDPHTPHPVIDLMDEQRDVIDMGGTMRLGIYPARLEPGSIVSAAYGEEVIYERHRHRYEVSNKHRPRLEEMGLVCSGTSPDGRLVEFVELPTDKHPFFVATQAHPEFKSRPDRPHPLFAAFVT"
  },
  {
    "reference": "MSDDNDKNSKKDDEPFDFFRLSVDDDNNDEKKPKGRKPFPFLVVLAIVVGAVLLINMFFSSKPDDLVDFSKFRGLIESGRITRIVIADPYYVGYGPESQAAVSAEGQKWSPFGFSSSDEDSGRPVYRTNGKWSEDFEKLLIEKGVEYKFESRQSGIFIQILFNLLPFILLFAIYFIAMRRLGAGMNSMGIFGGNGRSKAVDEGKVKTRFSDVAGVDEAKEELVEVVDFLKSPKKYTDIGGKIPKGVLLVGPPGTGKTLLARAVAGEAGVPFFRISGSDFVEMFVGVGASRVRDLFRTAREKAPCIIFIDELDAIGKSRMNNLGGNDEREQTLNQLLVEMDGFDNEKGLIILAATNRPDILDPALLRPGRFDRQVSVDKPDVKGREAILRIHSKNVKLDPSVDFDSLAHATAGFSGADLANIVNEAALLAVRGGRKQVTMPDFDEAIEKTVVGLEKKTRVMSEEERKSVAIHETGHALVTVFTKNTSPIHKISIIPRGVGALGYTMQRSEEEKFLNTEKDLINDIDILLGGRAAEQICLGVVDTGAANDIQRATGIIRSMITQYGMSKKFYNVTLGKSGRGYASGEPELVREYSEETQKYIDDEIARMMAERYKHVMALLTKHKDLLLYISDQLLEKETMTGKEFEEFVKDEANCVKAMKQKKASASKEKAPAKEKPVPSTKPAAKKRAGRPRKTSEA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.2. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation.3. The protein contains novel AAA+ ATPase domains that confer a unique function or activity.4. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.5. The ideal protein would be one that plays a critical role in protein catabolic process, cell division through zinc ion binding, ATP-dependent peptidase activity, ATP binding, ATP hydrolysis activity within plasma membrane.6. The protein must exhibit the following characteristics: Plays a role in the quality control of integral membrane proteins..7. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "MLWAAISLVMVVLFNLFNQPQTQSAKLSYSEFMQKVNAGDVVSVKIQGKKITGVATGGGKFLTYAPEDPSLVGSLMAKKIEVMAEA",
    "response#2": "MQLFYILLFVVLVLAASWFAGGQSSDQTKTLSQDQFITQLKQGKVKNFKLEPIGGAYQVTGEYKKAQKAKTTRSVSLFSRAQSSTSSEVTSFSSTVLPNNDTLKRINNAAMAEGVKTTAAPSENNGGGMMSMLLPILLLIGFGGAMMMQRNSSGKAMGFGKSRAKLLTERPGRVTFDDVAGIDEAKSELEEVVEFLKAPDKFTKLGARIPRGCLLVGPPGTGKTLLARAVAGEANVPFFSISGSEFVEMFVGVGASRVRSLFEQAKEAAPSIIFIDEIDAVGRQRGTGLGGGHDEREQTLNQLLVEMDGFEKNDAVVLIAATNRVDVLDPALLRPGRFDRQIVVDAPDRSGREAILEVHAKGKPLAKDVQLDVLARGTPGFSGADLENLVNEAALQAAKINKDRVDMADFENAVEKVQLGPAKKSRVISEKEKKTIAYHEGGHTIVGYYLKHADKVHKVTVVPRGQAGGYVVMLPQEDRFLMTKNELIDRITGLLGGRAAEEVVFGEISTGAHNDFEKATEIVRRMITEFGMSKKLAPMQFGRSQGQVFLGRDLGHEQNYSDAIAYEIDQEMQEIINACYDKAKQLLTEKREHLELVAQTLLTKETLDADEIKQLVDGGALEDPLDEEAEDGGSDDQPTLAPQA",
    "response#3": "MVMTEDEKKMTAYHEAGHAIVSLNEPASDPIHKATIIPRGRALGMVMRLPERDSYSYHRDKMLANLSVAMGGRVAEELIFGHDKVSSGASGDIQYATSLARSMVTKWGMSDKLGPLQYEESQEGYLGMGGSNRTMASDETNKLIDSEIRALIDNAHVRANQILTEKSDQLELLAQAMLEYETLSGDEIKERWQRVTEG"
  },
  {
    "reference": "MEAEELIGSSVTIDSIMSKMRDIKNKINEDCTDELSLSKICADHTETVNQIMRVGNTPENWLNFLLKLEKNSSPLNDDLLNKLIGRYSQAIEVLPPDKYGQNESFARIQVRLAELKAIQEPDDARDYFQMARENCKKFAFVHVSFAQFELSQGNLKKSEQLLHKAVETGAVPLQMLETAMRNLHLQKKQLLPEEDKKSVSASTVLSAQEPFSSSLGNVQNRSISCESRGQAGAARVLYGENLPPQDAEVRHQNPFKQTHAAKRSCPFGRVPVNLLNSPDFYVKTDSSAVTQLTTRLALSSVPLPYVTCLLHLQLLALAGLAKGSGPDRDAILPGSRPRGSDSYELRGLKPIQTIYLKDSLVSNEKSSELMSDLIALKSKTDSSLTKLEETKPEIAERRPMQWQSTRKPECVFQNPAAFAPLRHVPDVTPKADKESPPISVPKWLDPKSACETPSSSSLDDYMKCFKTPVVKNDFPPACPSSTPYSQLARLQQQQQQGLSTPLQSLQISGSSSINECISVNGRIYSILKQIGSGGSSKVFQVLNEKKQINAIKYVNLEDADSQTIESYRNEIAFLNKLQQHSDKIIRLYDYEITEQYIYMVMECGNIDLNSWLKKKKSINPWERKSYWKNMLEAVHIIHQHGIVHSDLKPANFVIVDGMLKLIDFGIANQMQPDTTSIVKDSQVGTVNYMAPEAIRDMSSSRENSKIRTKVSPRSDVWSLGCILYYMTYGRTPFQHIINQVSKLHAIINPAHEIEFPEISEKDLRDVLK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein with the reaction ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-   [protein], the Proton acceptor must be optimized to ensure efficient catalysis.2. The protein must exhibit the following characteristics: Phosphorylates MAD1L1 to promote mitotic checkpoint signaling ; Probably associated with cell proliferation ; Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint ..3. The ideal protein would be one that plays a critical role in protein localization to kinetochore through ATP binding, kinetochore binding, identical protein binding, protein serine/threonine kinase activity within cytoplasm, nucleus, kinetochore.4. The presence of Protein kinase domains is critical for the interaction of the designed protein with its intended targets.5. The ATP binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MKKLGCGTGVNVYLMNRMGKQTHSPWAIKKINSKCAQGQVSVYQKRLCEEAKILKDLKHPNIVGFRAFTTAKDGSKCLAMEFGGEQSLNDLIEKRREEGLQAFPVDTIEKVALHVARGLLYLHNEKKLLHGDMKSCNVVIKGDFESIKICDVGVSLPLDENMQVSDPKAHYIGTEPWKPKEALEDGVITDKADIFAYGLTLWEMMTLSVPHLEMLDTEGDEDDDDESFEEDFDEDAYYERLGSRPALDAVTLGGSYQRMVELFCLCTEEDPQKRPSAAHIVADLLIAEQEHSSNLRHPSLLQLMAVCLSRDLEKIRLVYERITVGTLFSVLHERRSQFPVLHMEMIVHLLLQIADALIYLHSRGFIHRSLSSYAVHIVSAGEARLTNLEYMMESQDSGAHRDMTRVPLPAQLYNWAAPEVVLQKTATVKSDIYSFSMIIQEILTDNIPWNGLDGSLVKETIALGNYLEADVRLPEPYYDIVKSGIHAKQKNRK",
    "response#2": "MKKLGCGTGVNVYLMNRMGKQTHSPWAIKKINSKCAQGQVSVYQKRLCEEAKILKDLKHPNIVGFRAFTTAKDGSKCLAMEFGGEQSLNDLIEKRREEGLQAFPVDTIEKVALHVARGLLYLHNEKKLLHGDMKSCNVVIKGDFESIKICDVGVSLPLDENMQVSDPKAHYIGTEPWKPKEALEDGVITDKADIFAYGLTLWEMMTLSVPHLEMLDTEGDEDDDDESFEEDFDEDAYYERLGSRPALDAVTLGGSYQRMVELFCLCTEEDPQKRPSAAHIVYTVERVAGQGTFGTVQLARDKSTGSLVAIKKVIQDPRFKNRELQIMQHLARLRHPNIVMLKNYFYTVGGEGRRNDVYLNVVMEFVPETLHRTCRNYYRRMTNPPLILVKVFMFQLLRSIACLHIPVINICHRDIKPHNVLVDEQTGELKLCDFGSAKRLAADEPNVAYICSRYYRAPELIFGNQFYTTAVDIWSVGCIFAEMLLGEPIFCGENTSGQLREIVKILGKPTKEELHKLNGSSTEINANAKATPWENVFKQPLPAEVYDLCGKIFKYVPDQRITPLDALCHPFVTEVR",
    "response#3": "MKKLGCGTGVNVYLMNRMGKQTHSPWAIKKINSKCAQGQVSVYQKRLCEEAKILKDLKHPNIVGFRAFTTAKDGSKCLAMEFGGEQSLNDLIEKRREEGLQAFPVDTIEKVALHVARGLLYLHNEKKLLHGDMKSCNVVIKGDFESIKICDVGVSLPLDENMQVSDPKAHYIGTEPWKPKEALEDGVITDKADIFAYGLTLWEMMTLSVPHLEMLDTEGDEDDDDESFEEDFDEDAYYERLGSRPALDAVTLGGSYQRMVELFCLCTEEDPQKRPSAAHIVFELGKKLGKGKFGKVYCVRHRSTGYICALKVMEKEEIIKYNLQKQFRREVEIQTSLNHPNLTKSYGYFHDEKRVYLLMEYLVNGEMYKLLRLHGPFNDILASDYIYQIANALDYMHKKNIIHRDIKPENILIGFNNVIKLTDFGWSIINPPENRRKTVCGTIDYLSPEMVESREYDHTIDAWALGVLAFELLTGAPPFEEEMKDTTYKRIAALDIKMPSNISQDAQDLILKLLKYDPKDRMRLGDVKMHPWL"
  },
  {
    "reference": "MAKVVHKFGGSSLSSPERYQAVAKIVLEQCQLGDSVVVSAAGKTTNTLVKLWQSFEQQDVRAFNDVLLQVENHQRELIDALFVGEQHSVLLKQLVNELQAIASGREEQHLHYASLLAHGELWSARILANYLSSQGIEAKAIDARTLFVQQEGVLLHAHNKEACYRAIDDHAIYIVTGFIAANAQGETVTLGRNGSDYSATLLASYLDAAQVSIWTDTTGVFSTDPRKVANAIKYTKVCRGQAELLARLGNPVLHAKTLSPLKDTDIKLQVRSSFDADALGTEIVKAGYSKEKRFITTFGQLDLLRVEQLKSGEVKQLSQLIQHSIHHFVKGGEAHLLVPSEFSHVVIKALDSRANIIESQVQGFAIVAPSQDVAGLHQQALELLDEHAIVTRFVLQERHYSLVLTDQAIDSDLLAILHERLVNKGRELAVIVAGVGNVGEVFIEQCKNQVAKLSEHFDLKLVGLLRSKQMLFCPSGLNLASWQQQWQNEAIEYNEAELFTRIKELDYEHKVVVDITASERFSELYPQFVEHDCHLISANKYAGTAQQGWYSKLRQQLQERNLLWRYNTSVGAGLPINFALADLQNSGDEVVRIEGVFSGTLSWLCSRYDGSEAFSELVLGAQELGFTEPDPREDLSGRDMQRKLLILARELDIELELDDIELEALMPESLSVGSWQTFLENRQQLDDFYATHYAKATANDAALRYTGALEIASNGKVSAKVGIAQVPNGSAIANLTPGDNIFVIHTKWYEQNPLVIQGPGAGKEVTAA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit aspartate kinase activity.2. We require a protein that can specifically bind to the metabolites produced in the Amino-acid biosynthesis, L-threonine biosynthesis pathway.3. The designed protein have Homoserine dehydrogenase catalytic domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MRVLKFGGTSLENAKKILLVKNIILTKLQNNKLAVVLSAPANITNNLSKLIDIAIINQPLQDKFLMMQNFLLNIVKDLKENINTLDIKNILDKINKIFSQLEYLFHYISISKHCSNKIHAKILSQGEQFSIIIMEALLIGSCNKVYVLSPKKFLLAESNNYLDSTVDLITSMRNIKKINFTHFNIILMAGFSAINKKNEIVLLGRNGSDYSAAALSACLHAKYCEIWTDVDGIYTADPNIVSNAILLKHLSYLETIALSYLGAKVLHHKTILPLLINNIPCVVKR",
    "response#2": "FEDLQQREGFSLVALVGNGVTAQAEQCLHFYHALNEQPLEFIQPAKNGLSLVGVLRKVVLEPLLLRLHQSLFSRPKRVGLILFGRGNIGQSWLTLFQEQQARLEKSLNLQVTLYAAFNSHGAMFDSAGLTREQIISDFEPSPLSWSSWLEGVTEHGFDHLIAIDMTASEQLAQRYPDLIAQHIHIIAANKLAVAAEQESYQTLKQHLVEQDVQLLSNATVGAGLPVQTSIKQLQYSGEQIQRIGGIFSGTLSWLFQQYDGSQPFSQLVQQAYQQGLTEPDPRADLSGQDVQRKLVILAREAGFALDPLAVHVENLVPATLAKLDRDSFFKRLSELDAPLAAAQAKAQQQGGVLRYLAQFDAATGEARVGLEVVDKDDAVAHVKAL",
    "response#3": "FEDLQQREGFSLVALVGNGVTAQAEQCLHFYHALNEQPLEFIQPAKNGLSLVGVLRKVVLEPLLLRLHQSLFSRPKRVGLILFGRGNIGQSWLTLFQEQQARLEKSLNLQVTLYAAFNSHGAMFDSAGLTREQIISDFEPSPLSWSSWLEGVTEHGFDHLIAIDMTASEQLAQRYPDLIAQHIHIIAANKLAVAAEQESYQTLKQHLVEQDVQLLSNATVGAGLPVQTSIKQLQYSGEQIQRIGGIFSGTLSWLFQQYDGSQPFSQLVQQAYQQGLTEPDPRADLSGQDVQRKLVILAREAGFALDPLAVHVENLVPATLAKLDRDSFFKRLSELDAPLAAAQAKAQQQGGVLRYLAQFDAATGEARVGLEVVDKDDAVAHVKAL"
  },
  {
    "reference": "MSELALLAGTANPALARRVAAHLDTPLREAVVARYPDGEIEVRIEESVRGQDCFVVQPTCPPVHEHLMELLVIADALRRASAARVTAVLPYFGYARQDRKMGPREPVTAKLVANLLTRAGVQHLLALDLHAGQLTGFFDIPLDHLSARMLLADYFLQKGLSDVVVVSPDIGGVRRAREFAQALRAPLAVIDKRRDRPNQVAEVVHVIGKVYRRTAILVDDIVDTAGTLVRAAETLLRRGVKEVYACCTHAVLSGPAVERIAASPIRELVVTDSIPLPPHKRLDRIRVISVARLVADAIARAHAHSSVSELAHEPLAAELVES",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should take into account the physiological conditions of the Metabolic intermediate biosynthesis pathway.2. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in nucleotide biosynthetic process. The designed protein sequence is ",
    "response#1": "VMVVSPDVGGVVRARALANRINAPLAIVDKRRPRAGESEVMNIIGDVTGYSCILVDDIVDSGGTLCNAAEALLANGATDVSAYITHGVLSGGAVARITASKLKELVVTDPR",
    "response#2": "LRDVATRTIQRIDTNIDKIVTAEAMGIPIATAISMSTDIPYVVMRKRQYFLEGEVPVHQETGYSKGELYLNGVEKGDRVTIVDDVLSTGGTLIAVIKALEKAGAEIVDIVCVIERGDGKSKVKF",
    "response#3": "LRDVATRTIQRIDTNIDKIVTAEAMGIPIATAISMSTDIPYVVMRKRQYFLEGEVPVHQETGYSKGELYLNGVEKGDRVTIVDDVLSTGGTLIAVIKALEKAGAEIVDIVCVIERGDGKSKVKG"
  },
  {
    "reference": "MGWEKDGQVSREVWEKAGQQGLLGVAIAEKHGGIGADILSSAVVWEEQMYVNCTGPGFSLHSDIVMPYIANYGSEEQIKHFIPKMTAGKCIGAIAMTEPGAGSDLQGIRTYAKKDGNDWILNGSKVFITNGWMSDVVIVVAVTNREAQSPAHGISLFLVENGTKGFIKGRKLNKIGLKAQDTAELFFEDVRLPASALLGKENKGFYYLMAELPQERLLIADLALASCEFMFEETRNYVKQRKAFGKTVAHLQTVQHKLAEMKTQICVGRAFLDNCLQLHADKRLDSPTASMAKYWASDLQNSIATQCVQLHGGWGYMWEYPIAKAFVDSRVQPIYGGTNEIMKELIARDIVSDKAMESDTALCMENSRAVEEKIKEDSIAQITCSVLGFPTAEPNLRNDILSVQHFGSPPSSKRYQSVLLMSANSAFNNKTGKQTKVGEPGCSRMGKSLHNGADASAGLISHSEPEEQVKGQMISETTSLNLAEVQRLSESNVTESSNAEEMQLLNGKWYKKNGFLGRALEVCAETMKGDLLHQILHGPSEGILSCTPEEVYARLLQCVTKQQMEISRAKRTQKRLQMLLAKHVIKHCDQQLKCFVKHHLQRMKVFREPTRCLSSSSLRCTEGWPENSTTPLESSSSTDPQNGVNIAPGEIRGFALHTGGLLSRVEKDLDSDATCSSSDEDGEEQTVRATVEASYTSEWKWLADRARIGSRWTWLQAQITELEYKIQQLTDLHRQIRATKGMVILEEFPFPKDILKKQIQLTDQEALL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein have PEHE domains with specific sequence motifs that are crucial for its activity.2. A protein with flavin adenine dinucleotide binding functions is required to enable long-chain fatty acid catabolic process.3. The protein design should incorporate a binding pocket that specifically recognizes FAD to enhance enzymatic efficiency.4. The protein should exhibit Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "PELGEAAQVLLDHWPGRARGFHRVDLPGFDAVLDLAVMDVVEALEAWDGLADAWFLDGFSPALNPAMWRDEVLAAVGARSAPGARAATFTVAGAVRRGLSAAGFEIAKRPGFGRKRERLEAWL",
    "response#2": "PELGEAAQVLLDHWPGRARGFHRVDLPGFDAVLDLAVMDVVEALEAWDGLADAWFLDGFSPALNPAMWRDEVLAAVGARSAPGARAATFTVAGAVRRGLSAAGFEIAKRPGFGRKRERLEAWLG",
    "response#3": "PELGEAAQVLLDHWPGRARGFHRVDLPGFDAVLDLAVMDVVEALEAWDGLADAWFLDGFSPALNPAMWRDEVLAAVGARSAPGARAATFTVAGAVRRGLSAAGFEIAKRPGFGRKRERLEAWV"
  },
  {
    "reference": "MSITAERKQALIKEYATKEGDTGSPEVQVAVLSERIANLTEHFKGHKNDNHSRRGLLKLVSQRRRLLDYVKGVDHARYQALITRLGLRR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a specific function within the cytoplasm, ribosome.2. The protein must exhibit the following characteristics: Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome.. The designed protein sequence is ",
    "response#1": "GQV",
    "response#2": "MDQNAI",
    "response#3": "MQPAP"
  },
  {
    "reference": "MNIDNASNWDPQALGLVPMVVEQSGRGERAYDIYSRLLKERVIFLVGPVNDVTANLVVAQLLFLEAENPDKDIYFYINSPGGSVTAGMSIYDTMQFIKPDVSTLCIGQAASMGAFLLNAGAKGKRFALPNSRVMIHQPLGGFQGQASDIAIHAKEILSIRDRLNRIMAEHSGQPLERIEKDTDRDNFLSAAEAAEYGLIDKVLTRRDAA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Plays a major role in the degradation of misfolded proteins.; Cleaves peptides in various proteins in a process that requires ATP hydrolysis.2. The protein must have high specificity towards serine-type endopeptidase activity. The designed protein sequence is ",
    "response#1": "MPKNILMIGPTGVGKTEIARRLAKLAGAPFVKVEATKFTEVGYVGRDVEQIIRDLVEIAISLVREKKRDEVQERAHINAEERVLEALVGKTASPATRDNFRQKLRAGELDDKEIEIEVANNSNNSAPTFDIPGMPGAQMGIMNLSDIFGKIGGRTKIRKTTVKDAFKPLIDDESEKLLDQDQIIQEALCITENDGIVFIDEIDKIATQDGGAGAAISREGVQRDLLPLVEGTIVATKYGQIKTDHILFIASGAFHVSKPDFVYASAAKTREYLGKRRYFSEIHERTDRPGIVTGLVWTPVGGDIIFIEATKMTGRGNFALSGQLGDVMKESARAALSWVRAEGEAYGIDPNFAQHYDLHVHVPAGAQPKDGPSAGIAMATALVSLLTGRVLRDDVAMTGEITLRGKVLPIGGVREKVLAAHRAGIRTIILPQRNLADLDEIPADVL",
    "response#2": "MPKNILMIGPTGVGKTEIARRLAKLAGAPFVKVEATKFTEVGYVGRDVEQIIRDLVEIAISLVREKKRDEVQERAHINAEERVLEALVGKTASPATRDNFRQKLRAGELDDKEIEIEVANNSNNSAPTFDIPGMPGAQMGIMNLSDIFGKIGGRTKIRKTTVKDAFKPLIDDESEKLLDQDQIIQEALCITENDGIVFIDEIDKIATQDGGAGAAISREGVQRDLLPLVEGTIVATKYGQIKTDHILFIASGAFHVSKPD",
    "response#3": "MPKNILMIGPTGVGKTEIARRLAKLAGAPFVKVEATKFTEVGYVGRDVEQIIRDLVEIAISLVREKKRDEVQERAHINAEERVLEALVGKTASPATRDNFRQKLRAGELDDKEIEIEVANNSNNSAPTFDIPGMPGAQMGIMNLSDIFGKIGGRTKIRKTTVKDAFKPLIDDESEKLLDQDQIIQEALCITENDGIVFIDEIDKIATQDGGAGAAISREGVQRDLLPLVEGTIVATKYGQIKTDHILFIASGAFHVSKPDFVYAARKAKTREYLGKRRYFSEIHERTDRPGIVTGLVWTPVGGDIIFIEATKMTGRGNFALSGQLGDVMKESARAALSWVRAEGEAYGIDPNFAQHYDLHVHVPAGAQPKDGPSAGIAMATALVSLLTGRVLRDDVAMTGEITLRGKVLPIGGVREKVLAAHRAGIRTIILPQRNLADLDEIPADVL"
  },
  {
    "reference": "MLKLGKVETYIHEKLEREKLHFVLLDPDDVSPELAGELASMSEEVGVDAIMVGGSTGAEGEVLDSVVRAIKESSNLPVILFPGSHGGISKYADAIFFMSLLNSRNPFFITGAQALGAFQVKRYGIEPIPMAYLIIEPGETVGWVGDAKPIPRHKPKIAAAYALAGQYLGMRLVYLEAGSGAPQPVPPEMIGLVKRVIDVPLIVGGGIRTEEQARAAVKAGADIIVTGTAIEKAGSVEKAREKLEELNRGVKG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a low Km value for (2E,6E,10E)-geranylgeranyl diphosphate + sn-glycerol 1-   phosphate = diphosphate + sn-3-O-(geranylgeranyl)glycerol 1-   phosphate.2. The protein requires Mg(2+) to activate a critical catalytic site.3. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+), sn-glycerol 1-phosphate binding.4. The protein must exhibit the following characteristics: This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.; Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P).5. The designed protein should be involved in glycerophospholipid biosynthetic process by localizing to cytoplasm and exhibiting phosphoglycerol geranylgeranyltransferase activity, imidazoleglycerol-phosphate synthase activity.6. The protein design should consider the endogenous proteins and enzymes present in the glycerophospholipid metabolism, Membrane lipid metabolism pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MELNWQDFITIIFILAISVYLIVDAIRKRKTGKKFGGGSGPMMDFKDKKTKKSGKKQKGPPPQDFSSGGMSEDFEGMGHDFEGPHHDSGYDDDFEEEPRPAKKAAPAKAAPAKAAPAKAAPAKAAPAKAAPAKAAPAGAEEESFEEEPVEEEPVEEEEEAEEEEEEAEKEESGEEEASEEEAEEEEEAEEEEDEEEAAEEEAEEEEEEEEDEDEEEEDEEEAEEEAEEEAEEEEEEEEEDEEDEEEEEEVEVEEEEEEEEEEEVVEEEEEVVEEVEEVEEEEEEEEEEEELEEEEGETTISTKKKKKKGKKK",
    "response#2": "MVLMASSSPPSSSPQQQQQQQSQTSQPHPQPPSSSSSQQQPSPPKQQSPQQQQQPPQQQQQQQQQPPQQQQQQQPPQQQQQPPQQQQQPPQQQQQPPQQQQQPPQQQQQPPQQQQQPPQQQQQQPPQQQQQPPQQQQQPPQQQQQPQQQQQPPQQQQPQQQPPQQQQQPPQQQQQPPQQQQPSQQQQPQQQQPQQQQPQQQQPQQQQPQQQQPQQQQPQQQQPQQQQQPPPPQQQQQQPPPPQQQQPPQQQQPQQQQPQQQQPQQQQQPQQQQPQQQQPQQQQPQQQQPQQQQPQQQQPQQQQPQQQQPQQQQPPQQQPPQQQPPQQQQQPPQQQPPQQQPPQQQQPPQPPQQQPPQQQPPQQQQQQQQQQQQQPPPQQQPPQQQQPPQQQQPQPPQQQPPQQQPPQQQPPQQQPPQQQPPQQQQPPQQQQPPQQQQQPPQQQQPPQQQQPPPQQQPPPQQQPPQQQPPQQQQPPQQQQPPPQPPPQQPPPQQQQPPQQQQPQQQQPPQQQQPPPQQQPPQQQPP",
    "response#3": "MVSFKLLSVISLAAISTVYAQNTTNGSGGNGSGGNGSGGNGSGGNGSGGNGSGGNGSGGNGSGSGDNGANGSYDSYESYYASGATQYDYSKYGTSTSGTNGTGANGTNGENANTANANANANANSNANANANANANANANGGSNGNGNGNGNGNGNGNGNGNANANANGNAGGNGNANGNGNGNGNGNSNGNGNGGGSNSQGGNGNGNSNGNGNGNGNGNGNGNGNGNGNGNGGGSNSNGNGNGNSNGNGNGNGNGNGGGSNSQGGNGNGNSNGNGNGNGNGNGNGNGGGSNSQGGNGNGNSNGNGNGNGNGNGNGNGGGSNSQGGNGNGNSNGNGNGNGNGNGNGNGNGNGNGNGGGSNSQGGNGNSNGNGNGNGNGNGGNGNRGGNGNSNGNGNGNNQSNTPTSTPSSS"
  },
  {
    "reference": "MNGGIPFRHLLLVLQLALLPAVTHGKTVVLGKKGEVVELPCETSLKKNVPFHWKTSDQIKILGVQNYFVTRGQSKLTDRIDSKKSSWDRGSFPLLIKDARIEDSETYICEVESKKEEVELQVFGLTANPDTHLLQGQSLTLTLESPPGSSPSVECTSPRGKRIRGRKTLSVSQLGIPDSGTWKCTVFQHLELVFEINIVVLAFQQASSTVYKKEGEQVEFSFPLAFAAETLTGSGELCWQAERASSSKSWITFNLTKQEVYVKLVTQDPKLRMGKKLPLHLTLAQALPQYAGSGNFTLALKGKTGKLHQEVNLVVMRVTQLQNNLTCEVWGPTSPKLMLSLKLENQEAKVSKREKAVWVLNPEPGAWQCLLSDSGQVLLESKFEALPTRSPPVQPMVLIVLGGVAGLLAFTGLGIFLCVRCRHRRRQAERMSQIKRLLSEKKTCQCPHRFQKTCSPI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should contain a Helical membrane-spanning region.2. The protein must exhibit the following characteristics: In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway; The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins; Participates in the development of T- helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.; Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs); Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses; In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.3. Incorporate a signal peptide in the protein design.4. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.5. The ideal protein would be one that plays a critical role in cell adhesion, T cell differentiation, adaptive immune response through MHC class II protein complex binding within plasma membrane, cell surface.6. The designed protein should contain one or more Ig-like V-type domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MLLLPLLLLAVIVPGGDNEDVFQGPTSFHVIQISTFANSTWAQNQGSGWLDNLQLYGWDSDPGTTIFLKPWSKGNFSDEEVTELEELFRVYLIGFTLEVQDHVSEFQLEYPFVIQDIAGCELHPGKAVESFLKGAFGGLDFVSIKNDSCAPVPEGGSMAQRFYELIIQYHAICDTIAKLLLETCPRYFLSVLDAGKASPPSVRVAQTTPFNTREPVMLACYVWGFYPADVTITWMKNGQLVPSHSNKEKTAQPNGDWTYQTVSYLALTPSYGDVYTCVVQHSGTSEPISTAATKVSNTFYAREGDQVEFSFPLSFEDENLVGELRWQAQGASSSLLWISFTLENRKLSMKEAHAPLKLQMKESLPLRFTLPQVLSRYAGSGILTLNLAKGTLYQEVNLVVEPVPPSLSVQPNHTVHSGETVSLLCWSMDSVDTFILSKEGSAQQPLRLKSKSHDQQSQAEFSMSAVTSHLSGTYRCYGAQDSSFYLLSSASAPVELTVLVRDDSPPSVRVAQTTPFNTREPVMLACYVWGFYPADVTITWMKNGQLVPSHSNKEKTAQPNGDWTYQTVSYLALTPSYGDVYTCVVQHSGTSEPISATAELTVQGEKFTVTGLQRPVLAPLGGNVELSCQLSPPQQAQHMEIRWFRNRYREPVYLYRNGKDLHGETISKYVERTELLKDDIGKGKVTLRIFKLTADDDGSYHCVFKVGEFYEEHISAWILVPGI",
    "response#2": "MLLLPLLLLAVIVPGGDNEDVFQGPTSFHVIQISTFANSTWAQNQGSGWLDNLQLYGWDSDPGTTIFLKPWSKGNFSDEEVTELEELFRVYLIGFTLEVQDHVSEFQLEYPFVIQDIAGCELHPGKAVESFLKGAFGGLDFVSIKNDSCAPVPEGGSMAQRFYELIIQYHAICDTIAKLLLETCPRYFLSVLDAGKAPPSVRVAQTTPFNTREPVMLACYVWGFYPADVTITWMKNGQLVPSHSNKEKTAQPNGDWTYQTVSYLALTPSYGDVYTCVVQHSGTSEPISWRTPAVTPVREVVLGKAGDAVELPCQTSQKKNIHFNWRDSSMVQILGNQGSFWTVGSSRLKHRVESKKNLWDQGSFPLVIKDLEVADSGIYFCDTDKRQEVELNVQYVAPSLSVQPNHTVHSGETVSLLCWSMDSVDTFILSKEGSAQQPLRLKSKSHDQQSQAEFSMSAVTSHLSGTYRCYGAQDSSFYLLSSASAPVELTVNYAANVPRYLLGVLDAGKAELHRQVKPEAWLSSGPTPGPGRLLLVCHVSGFYPKPVRVMWMRGEQEQPGTQQGNIILNADWTWYLRVTLDVAAGEAAGLSCRVKHSDPVELIVREAPLTVKCTYSVSGNPYLFWYVQYPNRGLQFLLKYITGDNLVKGSYGFEAEFNKSQTSFHLKKPSALVSDSALYFCAT",
    "response#3": "MLLLPLLLLAVIVPGGDNEDVFQGPTSFHVIQISTFANSTWAQNQGSGWLDNLQLYGWDSDPGTTIFLKPWSKGNFSDEEVTELEELFRVYLIGFTLEVQDHVSEFQLEYPFVIQDIAGCELHPGKAVESFLKGAFGGLDFVSIKNDSCAPVPEGGSMAQRFYELIIQYHAICDTIAKLLLETCPRYFLSVLDAGKAEEQLYQPLRDRDDAQYSRLGGPRYLLGVLDAGKAELHRQVKPEAWLSSGPTPGPGRLLLVCHVSGFYPKPVRVMWMRGEQEQPGTQQGNIILNADWTWYLRVTLDVAAGEAAGLSCRVKHSPNITESGNPSADTKRITCFASGGFPKPRFSWLENGRELPGINTTISQDPESELYTISSQLDFNTTRNHTIKCLIKYGDAHVSEPVILETSEVAWFVGGVIAAVLVLIVLILALMLCMKRKRDQLYQPLRERNDAQYSRLGDSESTCVLDDAGTPQDFTYCVSFNKDLLACWDPIVGKIVPCEFGVLYPLAENFSRILNKEESLLQRLQNGLPDCASHTQPFWIQPEDQVNVAEGNPLTVKCTYSVSGNPYLFWYVQYPNRGLQFLLKYITGDNLVKGSYGFEAEFNKSQTSFHLKKPSALVSDSALYFCAV"
  },
  {
    "reference": "MKYVVVTGGVLSGLGKGVTASSIGVLLKSAGLRVTSIKIDPYLNSDAGTMSPFEHGEVFVLDDGGEVDLDLGNYERFLDVALTRDNNITTGKVYAKVIEKERKGDYLGKTVQVVPHITNEIQDWIESVAHVPADGTSNTPDACVIELGGTVGDIESAPFIEALRQFQFRAGKGNVCFVHVSLVPVMGPVGEQKTKPTQHTVKELRGLGIIPDILVCRSENPLDSETKSKLAAFCHVGEDAVVSAHDVSNLYQIPISLHNQSVLGMVSNNLGFSVPESLPLLDEWKAMADKFDMLEDEVRIAMVGKYTGLSDSYLSVIKALQHSALAVNRKLVIDWIESTNLDPIEKTQAYDDAWATLKAADGILVPGGFGNRGVEGKIAAANYARVNNVPYLGVCLGLQIATIEFCRNVLGLEGANSAEFDENSPVHAVVFMPEISKTHMGGTMRLGTKPTPFLVDDCKVRRLYGGASHVDERHRHRYEVNPDLIERIESAGMVYVGKDETGERCEIMELEGHPYYVGTQYHPEFKSRPGKPSPPFLGLLKASCGQEI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be able to bind Mg(2+), ATP, CTP ligand in a variety of conditions2. The designed protein must possess metal ion binding to support 'de novo' CTP biosynthetic process under certain conditions.3. The protein design should consider the endogenous proteins and enzymes present in the Pyrimidine metabolism pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "LPDAYLSVTEALRAGGFAHHAKVAIRWVPSDTCETAAGAAEALDGVDAILVPGGFGIRGIEGKLGAIRHARTRKIPTLGLCLGLQCMVIEVARDIAGLEGASSTEFDPETPYPVISTMAEQRDVVAGERDMGGTMRLGAYPAVLERGTVAAQAYGTREVSERHRHRFEVNNSYRPKLAEAGLVFGTSPDGTLVEFVELPAATHPFFVGTQAHPELKSRPTRPHPLFAAFVL",
    "response#2": "LPDAYLSVVEALRAGGFANRAKVNVVWVPSDDCSTADGAAKVLSEVDGICVPGGFGIRGVEGKVGAIRYARENQIPILGLCLGLQCMVIEVARDLAGLAGADSSEFNPGTEHPVIATMAEQVAIVSGEGDLGGSMRLGSYPATLAEGSLVAKRYGSTDVAERHRHRYEVNNAYRDKLEAAGLVISGTSPDGRLVEFVELSTDLHPYFVATQAHPELKSRPTKPHPLFAGLIY",
    "response#3": "LPDAYLSVTEALRAGGFAHHAKVAIRWVPSDTCETAAGAAEALDGVDAILVPGGFGIRGIEGKLGAIRHARTRKIPTLGLCLGLQCMVIEVARDIAGLEGASSTEFDPETPYPVISTMAEQRDVVAGERDMGGTMRLGAYPAVLERGTVAAQAYGTREVSERHRHRFEVNNSYRPKLAEAGLVFGTSPDGTLVEFVELPAATHPFFVGTQAHPELKSRPTRPHPLFAAFVE"
  },
  {
    "reference": "MKIALIAHDKKKQDMVQFTTAYKDILSHHELYATGTTGLKIQEATGLSVERFQSGPLGGDQQIGALIAANALDLVIFLRDPLTAQPHEPDVSALIRLCDVYAIPLATNMGTAEILVRTLDGGAFDFRNLVRGGEPNV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein have MGS-like domains with specific sequence motifs that are crucial for its activity.2. The designed protein should have a high specificity for substrate binding, with minimal off-target effects.3. The protein must exhibit the following characteristics: Catalyzes the formation of methylglyoxal from dihydroxyacetone phosphate..4. A protein with methylglyoxal synthase activity functions is required to enable methylglyoxal biosynthetic process.5. The Proton donor/acceptor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "MSKVKNIAVVAHDNCKKELLDFVDCNHNILSRHNLVATGTTGGLVEKMIMERVEQKADEGYEFKPVNRMKSGPLGGDQQLGAMISEGKIDVLIFFWDPMQPQPHDVDVKALLRLAVLYNIPTASNRSTAEFLISSPFFEGEFQRKETDFSSYTQRKD",
    "response#2": "MEGQRCIALIAHDEKKDDMADFARHHQKVLASFRIVATGTTGGRVQEACPGLEVIRLKSGPLGGDQQIGAMIATGEVDMLIFFTDPLTAMPHDVDVKALTRLATVYDIPMALNRATAENLIDFNSAI",
    "response#3": "MEGQRCIALIAHDEKKDDMADFARHHQKVLASFRIVATGTTGGRVQEACPGLEVIRLKSGPLGGDQQIGAMIATGEVDMLIFFTDPLTAMPHDVDVKALTRLATVYDIPMALNRATAENLIDFNSAS"
  },
  {
    "reference": "MLPPGRNGTAYRARFGQQQRAQGGTVGGSEGAAPLGPAQVVTAGLLTLLIVWTLLGNVLVCAAIVSSRHLRAKMTNVFIVSLAVSDLFVALLVMPWKAVAEVAGYWPFGAFCDVWVAFDIMCSTASILNLCVISVDRYWAISRPFRYERKMTQRVALVMVGLAWTLSILISFIPVQLHWHRDKAGSWGGMDPPSNLANGTPWEEAGEPEVRAENCDSSLNRTYAISSSLISFYIPVAIMIVTYTRIFRIAQAQIRRISSLERAVEHAQSCRSRAACAPDTSLRTSIKKETKVLQTLSVIMGVFVCCWLPFFILNCMVPFCSGHPEGPRAAFPCVSETTFDVFVWFGWANSSLNPIIYAFNADFRKVFAQLLGCSHLCSRTPVETVNISNELMSYNQDTVFHKEIAAAYVHMIPNAVAPGHEEVDKEEEEGPFDRMSQISQTSPNGDPAADSVWELDCEREISLGKITTFTPNGFH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should be able to enhance the efficiency of reactive oxygen species metabolic process. The designed protein sequence is ",
    "response#1": "FEPYNKGEQSKFSNFKLAVAIKNINRITGNPNVNIGYATPENSRSSIMDFKFTTRFPDVIARLLPGSGKYQVLYTDYENFAILWSCGSIGSLGHSDQIWILGRDRDFEVDIRSKVYDVLKRLSLDPERLIID",
    "response#2": "MKPHHAAKSWTFLNFQSEDYTAVLMEFTTPKSYANTTVNIGIVCDTKNILSVTVDNDAEYIDPEVDSVGWPVPKEISVSFNGINADIKDDDVESADPFNTIVKGKLDNLVERVDVMAEIPTFVKNIVSGVAGTKPYIYQFFDDFELTIKDQESKGFGWCEITFIQ",
    "response#3": "MKPHHAAKSWTFLNFQSEDYTAVLMEFTTPKSYANTTVNIGIVCDTKNILSVTVDNDAEYIDPEVDSVGWPVPKEISVSFNGINADIKDDDVESADPFNTIVKGKLDNLVERVDVMAEIPTFVKNIVSGVAGTKPYIYQFFDDFELTIKDQESKGFGWCEITFIR"
  },
  {
    "reference": "MINIEDISKSSNQSEEKQLKSTSTSSKPKYSFVAKSLFKGSNNITPYYLSTSNTFQCVASESIQTWLLSDDGHIFTSSGNFVLDVSSGGYFVELVQLNSNSKTQIWTIDTTNNKIQPGNGKYLDIDSLNICVAPLNGNATQKWTTFRRAPIPTGNWGYFQSKQLDSNNNYWGLSVLNNSTSYNTSVVMNKVQAKSIGQIWQMTNDGHILSRLDGNLVLDIGPSIDGSKTNYHLNTNVYKANDLMQQWGINENNQIFNQYYPNLCIGFVGELGVDSTVNCVLAQPSSASDINFQWIANPTYSLNQIVSEVPEPFPAYTSGDLLASYQYLSDDATNGYTDDIRSLYTSINVNLEIFYVNVTNATCPSSIHSTEDFSYVQNQIKNELTYAINVRLVFDNYSGFYSKLFSQGSTNLTNLANLINVDMSSDQVVNGDYTDAITSVFYAIISEIPIGGSIIANISESAVQFGELYAESNDSGPSTYQVTLSKLYDHLNENYENEMANAQRIKNTILQDWGMMSKTFALCFLPTNNPSSLNINGLDFQKISTIASLAYQTAIIQMLLPTNYQIYFTPAGYYAPVSSDDYSYTDSTGTYIMAEIDNCNSHPPKALTGFKTRSFHIFLWLEFSYLCDLLQYGR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein have Ricin B-type lectin domains with specific sequence motifs that are crucial for its activity.2. The designed protein localizes to the cytoplasm. The designed protein sequence is ",
    "response#1": "IWDANRNNPVGENATLSLGRNGNLVLAEADGRVKWQTNTANKGVTGFQILPNGNIVLHDKNGKFVWQSFDHPTDTLLTGQSLKVNGVNKV",
    "response#2": "SPFVTSISGYSDLCMQAQGSNVWMADCDSNKKEQQWALYTDGSIRSVQNTNNCLTSKDHKQGSTILLMGCSNGWASQRWVFKNDGSIYSLYDDMVMDVKGSDPSLKQIILWPYTGKPNQIWLTC",
    "response#3": "YPFAAKSLFKGSNNIIPYYLSTSNTFQCVASESIQTWLLSDDGHIFTSSGNFVLDVSSGGYFVELVQLNSNSKTQIWTIDTTNNKIQNQGNGKYLDIDNLNICVAPLNGNATQKWTTC"
  },
  {
    "reference": "MTKKLFMFEKPLGMRDTLPFLYEIKKRVRQAMIREIETWGYELIETPTLEYYETVGTVSAIDDHQLFKLLDQQGHTLVLRPDMTAPIARLAASRLYKEGNPLRLAYNANVFRAQQREGGRPAEFEQIGVECIGDGTVAADAEVISVMIAALRQAGLQHFTVTIGHIGYVNALFLEIVGNEERASVLRRFLYEKNYVGYREHVKSLPLSSIDQKRLLQLLHLHGNDMTMEEAKELVHSEEGKRAVDDLCELSNALQLYGVDDVVKIDMTLVSHMSYYTGILFEVYAEHVGFPIGNGGRYDELLEKFSRKAPATGFGIRVDRLIEALGESEEEAAIECIVFSQERFAEAIELARTKRREGKRVVLQHISGIRDIDAYSKRYKPITYLLGSTEKE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that contributes to histidine biosynthetic process by functioning in cytoplasm with transferase activity.2. The protein must exhibit the following characteristics: May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine.; Required for the first step of histidine biosynthesis.3. Ideal protein should have a binding affinity for key enzymes in the L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9, L-histidine biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "ITVALAKGALLNDSVARFQSAGLDFSAVLDPGNRQLMVPSHCGRARALLVRNGDVPVYVAYGQAQLGVVGYDVLREHQMPVAQLVDLGFGGCRMAVAVKASSGYQHAADLPPHCRVASKFTHCAREFFDALDLPVELVHLTGSVELGPLTGIAEAIVDLVATGRTLRDNGLVAIEELFQSSARLRATDVPVYVEYGQAQLGIAGYDVLLEKSPDVANLIDLKFGYCRMSVAVPADSPYQSPLDIPHHGKVASKFVNCAKDYFRRLDIPVEIIPLYGSVELGPITGMSEAIVDLVSTGRTLRENGLVEIDELFASSARLIAHPLSYRLDRDQIYNWVEKLAG",
    "response#2": "RATDVPVYVEYGQAQLGIAGYDVLLEKSPDVANLIDLKFGYCRMSVAVPADSPYQSPLDIPHHGKVASKFVNCAKDYFRRLDIPVEIIPLYGSVELGPITGMSEAIVDLVSTGRTLRENGLVEIDELFASSARLIAHPLSYRLDRDQIYNWVEKLG",
    "response#3": "RATDVPVYVEYGQAQLGIAGYDVLLEKSPDVANLIDLKFGYCRMSVAVPADSPYQSPLDIPHHGKVASKFVNCAKDYFRRLDIPVEIIPLYGSVELGPITGMSEAIVDLVSTGRTLRENGLVEIDELFASSARLIAHPLSYRLDRDQIYNWVEKLL"
  },
  {
    "reference": "MAKFIFVTGGVVSSLGKGIAAASIGCLLESRGLKITLQKFDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTHARLTQNNNLTSGRIYERIITRERRGDYLGKTVQVIPHVTNEIKAAACKVSEGVDVVICEIGGTVGDIESQPFTEAIRQMRHELGRRNTLFVHVSYVPWIAAAQELKTKPTQHSVKELRSMGIQPDILICRSERPLPSDQRDKIALFCDVDRNAVISAYDVDTVYQVPVMFAEQGVDDLVVQALQLDSAGSRDLACWQAMLDRMRSPSDEVEIALVGKYVEYEDSYKSLKEALLHAGIHHRLKVRMSWIESEPLVYPDCAAQLGHYDGILVPGGFGKRGVEGMLAAIRFARQHHVPYFGICLGMQTAVIEFARNVCGLEGADSTEFEPATPYPVIYKLRDLLGVEELGGTMRLGAYECLLEDASFARAAYGQEVISERHRHRYEFNRAFEDRLTAAGLRITGRSRDAKFVEIVELPDHPWFLGCQFHPEFKSKPLEPHPLFKAFIGASYEYRQKRLQVETNPLFADEAR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein with metal ion binding, ATP binding is needed to enhance 'de novo' CTP biosynthetic process, glutamine metabolic process in a specific context.2. A key design requirement for the protein is the optimization of the active site, Nucleophile; for glutamine hydrolysis, Nucleophile to achieve high catalytic efficiency for H2O + L-glutamine = L-glutamate + NH4(+).3. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates..4. The presence of CTP synthase N-terminal domains is critical for the interaction of the designed protein with its intended targets.5. The designed protein should interact with enzymes and substrates within the Pyrimidine metabolism, CTP biosynthesis via de novo pathway pathway to enhance or regulate its activity.6. The designed protein should be able to compete with endogenous proteins or inhibitors for CTP, L-glutamine, UTP, ATP binding. The designed protein sequence is ",
    "response#1": "KYIFVTGGVVSSLGKGLAAASIGALLEGHGYRVTLQKLDPYVNVDPGTMSPYQHGEVYVTADGAETDLDLGHYERFTDTVTTKNHNWTTGKIYLSVIQKERRGDYLGHTVQVIPHITNEIKESMQLVASGEDIDIVLVEVGGTVGDIESQPYLEAIRQLRQDLGRENTLYIHLTLVPYIGTAGELKTKPTQHSVRDLRSIGIQPDILLCRTDRFLPQDIREKIALFCDVPVEAVITAKDVSSIYEVPLVLAEEGLDSIVLRGLTLECKEPDLTEWNGLIDAVHHPKHTVNIGLVGKYVELKDAYLSVAEALKHAGIDNSAEVDIAWIQSEDVNEENYEEVLKDCDGILVPGGFGDRGIEGKILAAKYARENKKPYLGICLGMQIAVIEFARSVLGYTDANSSEFNPKSNHAVIDIMEEQKEIGSKGGTMRLGSYPCKLIDGTKTKAAYGDLDTINERHRHRYEFNNKFREECQEKGLIISGLSPDNKLVEIIELKDHPWFVGCQFHPEFKSRPTRSHPLFKNLLRAAVEYQKSL",
    "response#2": "MTKYIFVTGGVVSSLGKGLAAASIGALLEGHGYRVTLQKLDPYVNVDPGTMSPYQHGEVYVTADGAETDLDLGHYERFTDTVTTKNHNWTTGKIYLSVIQKERRGDYLGHTVQVIPHITNEIKESMQLVASGEDIDIVLVEVGGTVGDIESQPYLEAIRQLRQDLGRENTLYIHLTLVPYIGTAGELKTKPTQHSVRDLRSIGIQPDILLCRTDRFLPQDIREKIALFCDVPVEAVITAKDVSSIYEVPLVLAEEGLDSIVLRGLTLSEHTEPDLSAWRDLVRRLRNPRHELKDAYISIAEALKHAGIYHQVKINLRRVDAGLLEDEGARKYLRDVQGILVPGGFGYRGIEGKIKAIKFAREEKIPFLGLCLGMQCAVIEFARNVCGLKGANSSEFNPRTPHPVIDLLLEQRKIKDMGGTMRLGLYPCQLKKGTFAYRAYKKELVQERHRHRYEVNNEYRPKMSKAGLIFSGLSPNNRLVEIVEVAEHPWFVATQFHPEFKSRPNRPHPLFREFVETAAGRHGER",
    "response#3": "KYIFVTGGVVSSLGKGLAAASIGALLEGHGYRVTLQKLDPYVNVDPGTMSPYQHGEVYVTADGAETDLDLGHYERFTDTVTTKNHNWTTGKIYLSVIQKERRGDYLGHTVQVIPHITNEIKESMQLVASGEDIDIVLVEVGGTVGDIESQPYLEAIRQLRQDLGRENTLYIHLTLVPYIGTAGELKTKPTQHSVRDLRSIGIQPDILLCRTDRFLPQDIREKIALFCDVPVEAVITAKDVSSIYEVPLVLAEEGLDSIVLRGLTLPDAYKSIIESLLQACAYNDRKLKLDLILSDKLTEENIEKKLEGVDGVIIAPGFGQRGIEGKLVTIKYTREKNIPTFGICMGMQCMVIEFARNVLGYADANSTEIDPTTTHNVVDIMEEQKNILNLGGSMRLGAFPCKLKKGTKVFKIYGKENISERHRHRFEFNNYYKEEFDKAGMICSGINASSNLVEIVEVPSHRWFIGVQFHPEYSSTVINPHPLFISFIETALNKTKKLLKDAYLSVAEALKHAGIDNSAEVDIAWIQSEDVNEENYEEVLKDCDGILVPGGFGDRGIEGKILAAKYARENKKPYLGICLGMQIAVIEFARSVLGYTDANSSEFNPKSNHAVIDIMEEQKEIGSKGGTMRLGSYPCKLIDGTKTKAAYGDLDTINERHRHRYEFNNKFREECQEKGLIISGLSPDNKLVEIIELKDHPWFVGCQFHPEFKSRPTRSHPLFKNLIEFTHKIA"
  },
  {
    "reference": "MAATQELSKGGVEEAVEEDDPAALKPGAAPFGNFPHYSRFHPPEQRLRLLPPELLRQLFPPEGPERRPILGLDVGCNSGDLSMALYKHFLSPHDGETSSGTSRELRLLCCDIDPVLVERAENGCRFPDALTFITLDIMDQESRKVPLSSFLSQFGRSVFDIVFCMSVTMWIHLNHGDRGLCEFLAHVSSLCSYLLVEPQPWKCYRAAARRLRKLGLHNFDHFRSLAIRGDMASQIVRILTQDHGMELACCFGNTSWDRSLLLFRAKHTEETQAIPESSTKETGTD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have a fast turnover rate for a 5'-end (5'-phospho)-ribonucleoside-RNA + 2 S-adenosyl-L-   methionine = a 5'-end (5'-bismethylphospho)-ribonucleoside-RNA + 2 S-   adenosyl-L-homocysteine.2. A protein that contributes to pre-miRNA processing, RNA methylation, tRNA methylation by functioning in cytosol, nucleoplasm, cytoplasm with RNA methyltransferase activity, O-methyltransferase activity, pre-miRNA binding.3. The designed protein have Bin3-type SAM domains with specific sequence motifs that are crucial for its activity.4. The protein must exhibit the following characteristics: O-methyltransferase that specifically monomethylates 5'- monophosphate of cytoplasmic histidyl tRNA (tRNA(His)), acting as a capping enzyme by protecting tRNA(His) from cleavage by DICER1; The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1; Was also reported to mediate dimethylation of pre-miR-145; however dimethylation cannot be reproduced by another group which observes a monomethylation of pre- miR-145.. The designed protein sequence is ",
    "response#1": "FMAALREPLPATLRITGYKSHAREILHCLKEKYFRELQHLEVDGQKVEMPQALSWYPEELAWHTNLSRKILRKSPQLERFHQFLVSETECGNISRQEAVSMIPPLLLNVNPDHKILDMCAAPGSKTAQLIEMLHADMNVPFPKGFVIANDVDNKRCYLLVHQAKRLNSPCIMVVNHDASSIPNLQVDVDGRKETLFYDRILCDVPCSGDGTMRKNIDVWKKWTTQNSLQLHGLQLRIATRGVEQLAEGGRMVYSTCSLNPIENEAVIASLLEKSQGALELADVSSELPGLKRMPGITKWKVMLKDGQWFEEWKDVPSNRQTQIRPTMFPIKEEEKLKAMNLERCIRILPHHQNTGGFFVAVLIKS",
    "response#2": "MDGWVYVLHPTPELWTLNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIRTIAPTGHLHTVEFHQQRAEKAREEFQEHRVGRWVTVRTQDVCRSGFGVSHVADAVFLDIPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAARGFSELSTLEVLPQVYNVRTVSLPPPDLGTGTDGPAGSDTSPFRSGTPMKEAVGHTGYLTFATL",
    "response#3": "FMAALREPLPATLRITGYKSHAREILHCLKEKYFRELQHLEVDGQKVEMPQALSWYPEELAWHTNLSRKILRKSPQLERFHQFLVSETECGNISRQEAVSMIPPLLLNVNPDHKILDMCAAPGSKTAQLIEMLHADMNVPFPKGFVIANDVDNKRCYLLVHQAKRLNSPCIMVVNHDASSIPNLQVDVDGRKETLFYDRILCDVPCSGDGTMRKNIDVWKKWTTQNSLQLHGLQLRIATRGVEQLAEGGRMVYSTCSLNPIENEAVIASLLEKSQGALELADVSSELPGLKRMPGITKWKVMLKDGQWFEEWKDVPSNRQTQIRPTMFPIKEEEKLKAMNLERCIRILPHHQNTGGFFVAVLIKEVTP"
  },
  {
    "reference": "MALLWALAVLSHLPLLDAQSPECANLMTVAPITNATMDLLSGKWFYIGSAFRNPEYNKSARAIQAAFFYLEPRHAEDKLITREYQTIEDKCVYNCSFIKIYRQNGTLSKVESDREHFVDLLLSKHFRTFMLAASWNGTKNVGVSFYADKPEVTQEQKKEFLDVIKCIGIQESEIIYTDEKKDACGPLEKQHEEERKKETEAS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have a specific function in regulation of immune system process and should be localized to extracellular space in order to carry out this function efficiently.2. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "LNPHNAARRQVGVGPMTWDNRLAAYAQNYANQRIGDCGMIHSHGPYGENLAAAFPQLNAAGAVKMWVDEKRFYDYNSNSCVGGVCGHYTQVVWRNSVRLGCARVRSNNGWFFITCNR",
    "response#2": "SGGGLVQPGGSMKLSCVASGFTFSNYWMNWVRQSPEKGLEWVAEIRLKSGYATHYAESVKGRFTISRDDSKSSVYLQMNNLRAEDTGIYYCTRPGVPDYWGQGTTLTVR",
    "response#3": "SGGGLVQPGGSMKLSCVASGFTFSNYWMNWVRQSPEKGLEWVAEIRLKSGYATHYAESVKGRFTISRDDSKSSVYLQMNNLRAEDTGIYYCTRPGVPDYWGQGTTLTVI"
  },
  {
    "reference": "MANYQNASNRNSSNKLVAPGAQAAIDQMKFEIASEFGVNLGPDATARANGSVGGEITKRLVQLAEQNLGGKY",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design must be able to perform double-stranded DNA binding in order to contribute to DNA topological change, sporulation resulting in formation of a cellular spore.2. The protein must exhibit the following characteristics: SASP are bound to spore DNA; They protect the DNA backbone from chemical and enzymatic cleavage and are thus involved in dormant spore's high resistance to UV light.; They are double-stranded DNA- binding proteins that cause DNA to change to an a-like conformation. The designed protein sequence is ",
    "response#1": "MSSSTEL",
    "response#2": "MEIIW",
    "response#3": "XTSL"
  },
  {
    "reference": "MLNSCINKIKKKKNIKKIILMLIFIFSVNFFTKNTNQGKQYEDKLNKDFTNIKKINIKNKLVNQKEFLLQLEKIKIFSPNLYSKNISIYNAILKWLKKRAEINELNKFRIKLFQMKGVDQYGNVKITGYYTPIVKASKIKKNNFIYPIYRTPSNFKKNEKLPQRKDIYNGFLKKEYILAYSDSLINNFIMEIQGSGFIDYGDNKPLIFFGYAKKNNWPYTSIGQILIKNGDIQKKNISMNTIKNWCTHHTQKEIQNLLEKNKSFVFFQETKRKEVYGSSAVPLVEKAAIAVDKSVIKIGSVVLVKIPVLDKNGIFIHKYEMHLLIALDVGGVIKGQHFDVYQGIGEKAGKLAGFYNHYGYAWVLKI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Murein-degrading enzyme; May play a role in recycling of muropeptides during cell elongation and/or cell division .2. The protein should have a fast turnover rate for Exolytic cleavage of the (1->4)-beta-glycosidic linkage   between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine   (GlcNAc) residues in peptidoglycan, from either the reducing or the   non-reducing ends of the peptidoglycan chains, with concomitant   formation of a 1,6-anhydrobond in the MurNAc residue..3. A protein that contributes to peptidoglycan turnover, cell wall organization by functioning in outer membrane with hydrolase activity, hydrolyzing O-glycosyl compounds, lyase activity. The designed protein sequence is ",
    "response#1": "MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEEPMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVFLFVAVPPMQREWIDLAAMSPYLPRSVVAAEDAHFCKHHGIDWGALREAIDDAKEDGTAFRGASTITQQVAKNLFLWQGRDFIRKALEFPLALWIDFVLPKPRILEIYLNIAELGPQGQFGVEAASAYAFGKSAASLSPREAALLASILPNPVKRSSL",
    "response#2": "FVKDTNGFEILMGQFAHNIENIWGLKEVLIAGPKDYVKYTDQYQTRSHINFDAGTITIETIATTNPAAHLRQAIITTLLMGDDPGSIDLYSDVNDIQISKEPFLYGQVLDNNGEPIRWEWRAAHFADYLLQNKMQTRTSGLHVISFVTIQLVPNHLDKRAHITGYYTPIVKASKIKKNNFIYPIYRTPSNFKKNEKLPQRKDIYNGFLKKEYILAYSDSLINNFIMEIQGSGFIDYGDNKPLIFFGYAKKNNWPYTSIGQILIKNGDIQKKNISMNTIKNWCTHHTQKEIQNLLEKNKSFVFFS",
    "response#3": "MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEEPMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLAATVLRVGTLNNQLSYYIGPDGQTGLDYELARQFADELGVKLEVKVAFRQAELFPMLKRGDIDLIATGLNQTPRAIKQFRPGPSYYYVSQVVVYKNGALRPRNLKQLVEYQNAKTASEDEAEASNNAAANTLQVVKQSQNVTLLKSLQSDYPELQFTTVSDADNYDLLRRVSTGELRFAISDSIELSLAQRLYPDLATAFEVTEDQPVSWFVRRSPDESLYALMIEFFGNISQSGELARLEEKYIGHAPLSYYSVNNQPSGFDYDLAQRFADYLGVTLKVRVRSNLNQLFDDLENDNADILAASLIYNAERLNRFTVGPSYYSVSQQLVYRLGQPRPKNLGDLRGRLAVASGSAQITQLRQLKKKQYPQLAWEVSSDLSSRSLLEKVADGKLDYTLADSASVGLLQRVHPQLAVAFDITEEKPVTWYLRRSDSEGLSAALLDFFSQLNDNGIMARLEEKYLGL"
  },
  {
    "reference": "MSVPIPLYSGEQVKSGEATAAKSKSVTMYELMTRAGQSVFEVVRSQYSNCHSLLIVCGGGNNGGDGYVVGKLAIEAGIDVTLWQCVESSRLKGDALQAYLDFISVGGRTELPSLDSVLDYTCILDALLGTGVIGNVRAEAFAIIEMLNQADVPVVSVDIPSGLCSNTGQVLGTCVQAEHTVSFIGLKQGLFTGRARDYTGKVHFAGLGVDDVFKQQNAPSSLLLNSIVDFAPVIARSRSAHKGSHGKAVIIGGNQGLGGAAILSALACQRAGAGLTALLTHPENTQPALVSCPEVMISSWLDSEVSDFRLNQWCDCFAIGPGLGMDVNVTKMFKALSFSTLPKVYDADALNLLAQNENYDAKRIITPHPGEASRLLKCSVEEIESDRFSAAEALYKKYGGVVVLKGAGTLIFDGKETYICNAGNPGMATGGMGDILTGVIVGLLVQGESLVNAAVRGVMIHSLAADRDAEINGERGLCARDLLPHIRHLVNEK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. The designed protein sequence is ",
    "response#1": "TPGALKKRFPVRPAAAHKGHTGHLLLLAGSPGKTGAAAMAATSAMRAGSGLVTLGIPRSLNPVLETMVTESMTVGLPETAEMALDESAWETVMSLIVDKRCLAVGPGLGTGRSTGRLLNRLIEASPVPLVMDADALNLIAANPSVLSKCPSPMVLTPHPGEMSRLSGHSTAEIQADRVGHARSFARQHRVHLVLKGAATVVADPDGTVFVNPTGNPGMAAGGMGDVLTGLIAGLLTQGMEAGAAARAGVYLHGAAADRLARRTAPTGYLATEVMNALPRTMQALLDV",
    "response#2": "PRAKKGDFGKLLIVGGSAEYSGAPALSALAALRTGVDMAVVAAPKAAADAIRGFSPNLIVRSLSGEHLARDDLPRLYELAAQSDGIVVGPGLGLEAETREAVIEFVKMARGPILVDADAIKALSAKPASLKGKRSVVTPHAGEFAILTGRAMADPPNIEERMRQVKDASKNLGTTILLKAHEDIISDGERLKLNLTGNPGMTVGGTGDVLSGIAGAFLAWGAEPFRAACAAAFVSGKAGDLAKSEKGYHIVATDVVEKK",
    "response#3": "LLIVGGSAEYSGAPALSALAALRTGVDMAVVAAPKAAADAIRGFSPNLIVRSLSGEHLARDDLPRLYELAAQSDGIVVGPGLGLEAETREAVIEFVKMARGPILVDADAIKALSAKPASLKGKRSVVTPHAGEFAILTGRAMADPPNIEERMRQVKDASKNLGTTILLKAHEDIISDGERLKLNLTGNPGMTVGGTGDVLSGIAGAFLAWGAEPFRAACAAAFVSGKAGDLAKSEKGYHIVATDVVEKL"
  },
  {
    "reference": "MIEKTYQPADIEARISRAWEDAEAFKAGRPERRDAVPYSIVIPPPNVTGSLHMGHALNNTLQDILCRFERMRGRDVLWQPGTDHAGIATQMVVERQLMERQEPSRRDMGRAKFLERVWQWKAESGGVIVNQLKRLGASCDWSRERFTMDEGLSRAVAKVFVELHRQGLIYKDKRLVNWDPKLLTAISDLEVQQIEVKGNLWHLRYPIEGKTFDPADPSSFIVVATTRPETMLGDSAVAVNPEDERYTHLVGKHVILPLVGRRIPIVADEYSDPEKGSGAVKITPAHDFNDFEVGKRHHLPQINVLDIEGKISVADNSAYLEGLPEGAREFAGEIDGTDRFVARKIIVARLDDFGFLEKIEPNVHMVPHGDRSGVVIEPFLTDQWYVDAKTLAQPAIAAVRSGETTFVPKNWEKTYFEWMENIQPWCISRQLWWGHQIPAWYGPDGKVFVAETEEEAVGNALGYYVEQEVITPAQAHDMAEDPAKREGFITRDEDVLDTWFSSALWPFSTLGWPDETPELDRYYPTNVLVTGFDIIFFWVARMMMMGLHFMDDVPFPTVYIHALVRDEKGAKMSKSKGNVIDPLNLIDEYGADALRFTLAAMAAQGRDIKLATSRVEGYRNFATKLWNACRFAEMNGCVAPAGFDYTAAKETLNRWIAHETVRAVREVTEAIESYRFNDAAEAAYRFVWNVYCDWYLELAKPVLMGEEGAAKTETRAMVAWARDEILKILHPFMPFITEELWAVTAPRDGLLALAPWSRKGGISDEEVS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Design the protein with a signal peptide incorporated.2. The protein should have a fast turnover rate for ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-   tRNA(Val).3. The protein design must feature the 'KMSKS' region, 'HIGH' region motif.4. A protein that can perform ATP binding, aminoacyl-tRNA editing activity, valine-tRNA ligase activity in cytoplasm to support valyl-tRNA aminoacylation.5. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.6. The protein must exhibit the following characteristics: As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner.; Catalyzes the attachment of valine to tRNA(Val). The designed protein sequence is ",
    "response#1": "MSKNNRWIIAETAKVARALDQHIEDMRYDELANSLYHFVWNDFCDWYLELIKPILTSTEDQNQGELQETLAVLGWVIDQILIMLHPIMPFITEELWHALGDRDHDLIVAAWPDYKNWSVDETAQNDIDWLIRLITAIRATRSELNVPPALKVS",
    "response#2": "MNRWIIAETAKVARALDQHIEDMRYDELANSLYHFVWNDFCDWYLELIKPILTSTEDQNQGELQETLAVLGWVIDQILIMLHPIMPFITEELWHALGDRDHDLIVAAWPDYKNWSVDETAQNDIDWLIRLITAIRATRSELNVPPALKVPKLYAPNVTGSLHMGHTGLDPSRIAGYRNFATKLWNATRFAKINGVKHNPAFKPEKVKLALNRWILTELSKTVSAVTTGIENYKFNESADALYRFIWNTLCDWYLELLKPVFQSFNEEAKNEAQACTAWVLDEVYKLLHPFMPHMTEELEFHTERVENARNFANKLWNASRFVLMNLADWQPAAEGAALQYDVADRWIRHRFNEAARAVNELLGEYQYGEAARTIYDFIWSEFCDWYIELVKPRLYNPADPTRAAAQETLARVLEGTLRLLHPFMPYITEAIWQKY",
    "response#3": "MTNDKWIESQFELAAKAFNNHIDNYRLDMAANTLYEFIWNQFCDWYLELTKPVLWKGTEAQQRGTRRTLITVLEKTLRLAHPVIPYITETIWE"
  },
  {
    "reference": "MTASQQQIQLARRPQGIPVHEDFRFETIPVPEPKQGEVLVKTLYVSVDPYMRGRMQDTKSYVEPFALDKALSGGVIAEVVSDGNHLKKGDIVIGNLSWQEFSAVSESALRKIDTSLAPASAYLGILGMTGLTAYFGLLDIGRPKEGETVVVSGAAGAVGSTVGQIAKIKGARVVGIAGSDEKIDYLKQELQFDEAINYKTADDIQKALQNACPDGVDVYFDNVGGPISDAVMNLLNEFARIPVCGAISSYNAESEADDMGPRVQSKLIKTKSLMQGFIVSDYSDRFSEGAKQLAEWLKAGKLHYEETITEGFENIPDAFLGLFKGENKGKQLIKVSDPS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design must be focused on enhancing the efficiency of response to toxic substance, aromatic compound catabolic process. The designed protein sequence is ",
    "response#1": "MSKPLKVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRLAALGGEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAMLAQMLQPGQPPNPYVQHPPFKFVVFVGGFRAEKPEFDHFYNPKLTTPSLHIAGTSDTLVPLARSKQLVERCENAHVLLHPGQHIVPQQAVYKC",
    "response#2": "MSKPLKVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRLAALGGEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAMLAQMLQPGQPPNPYVQHPPFKFVVFVGGFRAEKPEFDHFYNPKLTTPSLHIAGTSDTLVPLARSKQLVERCENAHVLLHPGQHIVPQQAVYKC",
    "response#3": "MSKPLKVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRLAALGGEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAMLAQMLQPGQPPNPYVQHPPFKFVVFVGGFRAEKPEFDHFYNPKLTTPSLHIAGTSDTLVPLARSKQLVERCENAHVLLHPGQHIVPQQAVYKC"
  },
  {
    "reference": "MRHYYSVEAIRAAEAPLLAALPDGVLMRRAAFGLTTVLAGELRARTGAVSGRRVCAVIGSGDNGGDALWAATLLRRRGVAADAVLLNPERTHPQALAAFRRCGGRLVDAVAPDTDLVIDGVVGISGRGPLRPAAAAVFAAAADIPVVAVDIPSGVDAHTGAVNGPAVQAAVTVTFGGLKPVHALADCGRVELVDIGLDLPDTDLLGFDASDAAERWPVPQRSDDKYTQGVTGVLAGSAAYPGAAILCAGAAVAATSGMVRYAGSAAAEVVAHWPEVVVADSAAAAGRVQAWVVGPGLGTGEHAAAALWFALGSDLPVIVDADALTLLAAHPDLVTERTAATVLTPHAGEFARLAGEPPGADRVGATRALADRFGATVLLKGHVTVIAAAGGPVYLNPAGGSWAATAGSGDVLSGMIGALLAAGLGPAEAAATAALVHAEAALRAAADPGPVPAPTSASRILAHIRPAVAAVRATGAAADPR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.2. The designed protein have YjeF N-terminal domains with specific sequence motifs that are crucial for its activity.3. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.4. The designed protein should be able to compete with endogenous proteins or inhibitors for AMP, (6S)-NADPHX, K(+) binding.5. We need a protein that can perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate with high efficiency.6. The designed protein must possess NADHX epimerase activity, NADPHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, metal ion binding, ATP binding to support nicotinamide nucleotide metabolic process under certain conditions. The designed protein sequence is ",
    "response#1": "MRLLDHAAIQGIGLPAAVLMENAGRAIAEEVLHLCRLCRFDKAEGHPGGRIIQRPPLAGLDLSASSSFPAELSRKGECEAVNVWKTEHWLILVGKGNNGGDGIVCGRHLAAAGIRVTLLYADPPAQFTGEAGLQRTIAEQLAIPHKVYERGTLRFTEYTGVVDALLGTGAKGAPRGMYAALIREVNDSGLPVVSADIPSGLNADTGEMHDPCIQAHRTVCLAFLKTGLTQYPGAGAAGEIVVRDIGIPEHLADDDVADLLPRPEAESDKYRRGVLGIVAGSDRFTGAAVLAVGGAIRAGAGLVRFASAETAVRQVRNRWPEAITTVLDGPSVAEVGRVQAWVLGPGMGTGEEAVALARAVLATDVPVLVDADGLTILANDRSLLRRSAPTVLTPHAGELSRLLDVPRERIEAARMEHVRRAVAELGAIVLLKGSTTLIAEQDRPVRVNTTGTPWLATGGTGDVLSGIAGTLLSGGLSGYDAVSCAAHLHGLAAQAASTGISGGHGPIAAMDVATMLPQVIPDSHKYTRGLVTIIAGDMPGASALAASAAMRSGAGVVRLQAREFVPGVPAAVIQMPGDPLDRLDDKRIGALLLGPGLRSGRELLDAALASGHPLVLDAGALAMLADGGPERIEGAILTPHDGEFQKLFGALEGSKVDRARASAAQSGAVIAYKGPDTVVAAPDGRAAIHGTAPPWLGTAGSGDVLAGIIAAERAAGKEPFEAACAGVWRHAEAARRAGPKMIADDLIT",
    "response#2": "MRLLDHAAIQGIGLPAAVLMENAGRAIAEEVLHLCRLCRFDKAEGHPGGRIIQRPPLAGLDLSASSSFPAELSRKGECEAVNVWKTEHWLILVGKGNNGGDGIVCGRHLAAAGIRVTLLYADPPAQFTGEAGLQRTIAEQLAIPHKVYERGTLRFTEYTGVVDALLGTGAKGAPRGMYAALIREVNDSGLPVVSADIPSGLNADTGEMHDPCIQAHRTVCLAFLKTGLTQYPGAGAAGEIVVRDIGIPEHLAEHLPRRPLDSHKGTFGHVLVVAGSRNYVGAAYLASQASVRAGAGLTTLATPESVYPVAAAKATEPIHMPLPEDEEGRVGAQAAESLRRGSRRYTNILVGCGLGLSEGTAKFVEDLLFGGEGSGLSGLPALVDADGLNNLARIAGWPERGRCHMVLTPHPGEMATLMGLQASEIQKHRVLVAREYAAKWNVCLVLKGANTVIGEPGGMVRVAPFANPGMATGGTGDVLSGVIAGLMAQGLAPAVAACCGVYLHGEAGRETASRLGNMGVAASDLLPEIPMAVGIVAGSRGFAGAATMCAWGALRAGAGLVEVFVPENIYEVVAASAPFESMVKPVTSYRDLLDEKIDVWGIGPGLGKENAREIIDLVQRTKNAMVVDADALNLISREVSILKNCRGPRLLTPHPGEMKRLAGDRKISRAKLAKNFTAEFPVTLLLKGSRTVVAENGKRISYNTTGNPGMATGGMGDVLTGVCASLIAQNLSPFDAARVGAWVCGRAAELAIS",
    "response#3": "MRLLDHAAIQGIGLPAAVLMENAGRAIAEEVLHLCRLCRFDKAEGHPGGRIIQRPPLAGLDLSASSSFPAELSRKGECEAVNVWKTEHWLILVGKGNNGGDGIVCGRHLAAAGIRVTLLYADPPAQFTGEAGLQRTIAEQLAIPHKVYERGTLRFTEYTGVVDALLGTGAKGAPRGMYAALIREVNDSGLPVVSADIPSGLNADTGEMHDPCIQAHRTVCLAFLKTGLTQYPGAGAAGEIVVRDIGIPEHLAVGVAAGSERYPGAAVLAVAGALRGGAGAVRYVGPPAEAVLARFPEVLIGPGRVQAWVAGSGLGDAPEARERLARALDDGVSTVVDADGLRMLGPAPGRTARGPLVLTPHAGEAAALLGVAREEVEEARLESVRQLARQTGATVLLKGSTTLIASPPGEADAGEEDGGTVLANLNGTGWLATAGTGDVLAGLIGSLLAAGLAPADAAAVGAHLHGLAEPSDIGAMLPARSVDAHKRSTGSVLVLAGSDGMSGAALLTSRGAARMGAGYVTLVSTSYVDHAKKSLIPEAVSRIVADHSELGPEVLAEFDDDFDRADAVALGPGLGTGPRQQALVETALEAIDVPLVLDADGLNVLAEDAAALRKRTSPTVLTPHPGEMATLLKLSSKDVQADRVGVARRAASDLGCVVVLKGAGTIVAGPEGPAVVNPTGGPELATAGTGDVLTGVVGALLAEGLDPVSAAIAGVFVHGLAGGVAGVSAGGRGTVAWDVAEALPAAVGELYG"
  },
  {
    "reference": "MHDIRAIRENPAAFDAALALRNLPPVSPEILSLDADRRSRIAAAEAAQAEQNKASKEAGAAKGRGDEAEFQRLRALVTAKKDETARLQAEATALDAKLRELLLAVPNLPLDGVPPGKDEDDNVEIRRWGEPRAFDFAPREHFEIEGVRPGMDFETAAKLSGSRFVVLKGGVARVHRALAQFMLDLHTEEHGLAETWAPVLVLSEMMEGTGQLPKFGEDSYQTREGWWLIPTSEVTLTNTVNGDLVDHASLPRRMVAHSQCFRSEAGSAGRDTSGMLRQHQFEKVEMVSITDAESGVEEHARMTRCAEAVLERLGLPYRTIVLCGGDMGFGARITHDLEVWLPGQGKYREISSVSYCGDFQARRMNARYRPAGGGKPEFVHTLNGSGLAVGRTLIAVLENGQQADGSVKLPEVLHPYLGGRTRLGADGVLA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Catalyzes the attachment of serine to tRNA(Ser) The designed protein sequence is ",
    "response#1": "DNVEQSRWGTPRQFDFKVLDHVELGARNGWLDGETAAKLSGSRFTVLRGPIARLHRALAQFMVDLHTGEHGYEETNVPLLVNADSLRGTSQLPKFEDDLFKTAVGDSTRYLIPTSEVPLTNIVRDEIVDAERLPLRMTAHSMCFRAEAGSGGRDVRGMIRQHQFEKVELVSISRPEDSDAEHQRMTRCAEVVLEKLGLPYRKVLLCTGDMGFSAVKTYDLEVWLPSQETYREISSCSNCGDFQARRMQARWRNPATGKPELAHTLNGSGVAVGRAMIAVMENYQNADGSITVPEALRPYMG",
    "response#2": "NVEIRRVGVPRSFDFQVKDHVDIGTPLGLDAETGAKLSGARFTVLRGQMARLHRALAQFMLNTHADEHGYTEVYTPYMVNATSMYGTGQLPKFEEDLFRVPRGDENFYLVPTAEVPVTNFVRDEIVKAADLPLRYVAHTPCFRSEAGAYGRDTRGMIRQHQFDKVELVQVVRPEESLNALETLTGHAETILKKLGLPYRVIVLCTGDMGFGSQKTYDIEVWLPAQDTYREISSCSSMGDFQARRMQARFKDEAGKNQLVHTLNGSGLAVGRTLVAVLENYQNADGSVTVPEVLRPYTR",
    "response#3": "DNVELRRWGSPRQEEGLEEHWQIAERLHLFDTERSVRIAQSRFVTLMGQGARLERALINFMLDLHTSKGYREVMPPVLVNSASLTGSGQLPKFADDCFRCSEDDLWLTPTAEVPVTSLHRDEIIPADQLPLRYAAYSPCFRREAGSYGRDTRGLIRLHQFNKVELYWFAHPDHSAEAHAQITADAEAVLQALELPYRVLDLCTADIGFSAQRTYDLEVWLPGAEAYREISSCSVCGDFQARRSAIRTKEGKSTKLVHTLNGSGLAVGRTMAAVLETGQQSDGSVLLPKALVPA"
  },
  {
    "reference": "MSVPRPAAVIVLAAGEGTRMKSATPKVLHELCGRALVDHMLAAARGLAPERLIVVIGHAREQVQAHLAASGPDARPVVQEEQNGTGHAVRCVLEAVGAISGTVLVTYGDTPLLRTETLAELLATHAGEGNAVTVLTAEVPDPAGYGRIVRDASGAVLAIVEDRDATPEQRAIREMNSGVYAFDGLLLADAVKRVSTANAQGEEYLTDVLAILRQDGHRVGAYVAADHVEVEGVNDRVQLSFARRVLNDRLLEAHMRAGVTVVDPATTWVDVGVTIEPDTVLHPGTRLHGRTSIGAGAQVGPDTTLTGTTVGAGAVVRNAVCDGAEIGEGASVGPYAYLRPGAVLARGAKAGTYVELKNARVGEGSKVPHLTYVGDATIGEGSNIGASSVFVNYDGVEKHHTTIGDHVRVGSDNMLIAPVTVGDGAYTAAGSVITNDVPPGSMAVARSRQRNIEGWVTRRRAGTKSADAARAAQAVAEAGGGEPGGPGEAVR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be optimized for activity in UDP-N-acetyl-alpha-D- glucosamine biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, Nucleotide-sugar biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The designed protein have MobA-like NTP transferase domains with specific sequence motifs that are crucial for its activity.3. The Mg(2+), UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA binding site should be located in a region of the protein that is accessible to the ligand.4. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate are properly positioned for optimal catalysis.5. The protein design should be able to enhance the efficiency of regulation of cell shape, lipid A biosynthetic process. The designed protein sequence is ",
    "response#1": "VVVMAAGKGTRMKSARPKVLHRLAGKALLGHVIERAAGLGARQAVIVTGHQAEQVEAALAAMNEQRLPWRCVRQSPQLGTGHAVQQAMPALPDDGVTLVLSGDVPLTEVATLQALLDACAGDIGLLTVVLDDPTGYGRIVRDAAGQVEAIVEHKDASAAQRAIREVNTGIMVAPTAALKRWLAALKNDNAQGEYYLTDIVAQAVADGVAVHTAQPAHAWETEGVNNKLQLAALERAWQTQAAERLMADGVTLLDPARLDVRGDVDCGRDVEIDVNVVLDGQVSLGDGVSVGANCVIKDSTIGPGTKILAGTHIEGADIGXQISIGPMARIRPGTVLGDGCKIGNFVETKNAVIGEGSKANHLAYIGDAVIGDGCNIGAGAVFCNYDGANKHQTKLGNNVFVGSNSVLIAPVTLDDNAFVAAGSSVSSNVPAGSLAVARVKQRHIQGWQRPVKKA",
    "response#2": "VVVMAAGKGTRMKSARPKVLHRLAGKALLGHVIERAAGLGARQAVIVTGHQAEQVEAALAAMNEQRLPWRCVRQSPQLGTGHAVQQAMPALPDDGVTLVLSGDVPLTEVATLQALLDACAGECVSLLTVELADPTGYGRIVRDAAGRVQAIVEHKDASEAQRAIREVYTGMMAVPARHLKRWLAQLSNANAQGEYYLTDIVALAVADGVPVVAHHIADAHQVAGVNSPAQLAELERWHQRQQAEALMTARVQLARCEASMQARLREAAMRGGATLVDPASVTFSFDTKLGRDVLVEPHVVFAPGVAVADGARIRAFSHLEGAEVGPGAIVGPFARLRPGAVLAEDVHVGNFVEVKASTLAAGVKANHLSYIGDADIGARTNVGAGTITCNYNGFQKFRTTIGADVFVGSDSALVAPVTIGDGAYIGTGAVVTEDVEADALAIARARQISIANWQRPVKKP",
    "response#3": "MSAVIMAGGLGTRMRSATPKHFHPLLGRRMVDWVVGAAAELAPEPLVVVTSPENLGAYDGVAVAVQKRALGTGDAVRSAAGLLEGFDGDVLILNGDVPALRPDVLRSLVEVHRDEQAAATVLSFEPKQARHYGRILRDGDRLLRIVEAVDASDEELVVPEVNSGMYVFRADALWPALERLRDAAGDASGEYYLTDVVALARADGLSVTALRTDDAQEVAGVNSRAELAAAERVLRERINRALMLAGVTVVDPRTTWIDADVSLGRDVTIHPQTQLLGASTIGSGVTLGPDTTLTDVEIADGASVSRTQGSLAVIGAGATVGPFAYLRPGTVLGVQGKIGTFVETKNAQIGDGAKVPHLSYVGDAEIGEGTNIGAGTIFANYDGVEKHHTTIGKHVKTGSHNTFVAPVHVGDGAATGGGAVVRRDVPPGALAVSTGPRVQLARCEASMQARLREAAMRGGATLVDPASVTFSFDTKLGRDVLVEPHVVFAPGVAVADGARIRAFSHLEGAEVGPGAIVGPFARLRPGAVLAEDVHVGNFVEVKASTLAAGVKANHLSYIGDADIGARTNVGAGTITCNYNGFQKFRTTIGADVFVGSDSALVAPVTIGDGAYIGTGAVVTEDVEADALAIARARKEIVPDWRTRNRR"
  },
  {
    "reference": "MEKFLNGLEYEILKDVVKEEEYTGIEYDSRKIKAGNIFVALEGAVSDGHNYIEQAVKNGAKCILVSKKIEAVFPVKYIWIKDLRRKLGVLASNFYNWPQKKLKIIGITGTNGKTTTTYLIESILGSDKTARIGTVEYKIGDEIIEAPNTTPESLDIVKMCKKSVEKGMEYLVMEVSSHALALGRVDMLEFDVSMFTNLTLDHLDFHKTMEDYFQAKRKLFTMMKKGCEKNCVINIDDLYGKRLSSEFGGISYGMHNEGRVRGKILEFHGDGQEVEINIDNFSTKIKLAILGRYNVYNVLGALSIALLLVIERDIILDKIKELKGAPGRYELVNCGQDFTVIVDYSHTGDALENILKSINELKKGKIITVFGCGGDRDPSKRSVMGEIAERLSDIAIVTSDNPRTEDPHKIVEQVLEGMKGKNHIVEEDRDIAISKAVKLAEAKDIILIAGKGHETYQILGRKKIHFDDREIARREIVKKKKMK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "IGAGLVTLAVPGAALQENAARLDAVMLTRMNDVRDLAEILEDDRLNALCLGPGLGHGGGTRALVHAALEVERPVVLDADAMTSFEEDPEELFDALHLGVVLTPHMGEFARLFPDLAAPLKESPEAGPAYSRLDAARLAAERAGCTILLKGPDTIIANAESAALHGAFYDRAAPWLATAGAGDTLAGFITGLLARDDDPFFAATAATWLHADCARDFGPGLIAEDLPERR",
    "response#2": "IGAGLVTLAVPGAALQENAARLDAVMLTRMNDVRDLAEILEDDRLNALCLGPGLGHGGGTRALVHAALEVERPVVLDADAMTSFEEDPEELFDALHLGVVLTPHMGEFARLFPDLAAPLKESPEAGPAYSRLDAARLAAERAGCTILLKGPDTIIANAESAALHGAFYDRAAPWLATAGAGDTLAGFITGLLARDDDPFFAATAATWLHADCARDFGPGLIAEDLPERR",
    "response#3": "IGAGLVTLAVPGAALQENAARLDAVMLTRMNDVRDLAEILEDDRLNALCLGPGLGHGGGTRALVHAALEVERPVVLDADAMTSFEEDPEELFDALHLGVVLTPHMGEFARLFPDLAAPLKESPEAGPAYSRLDAARLAAERAGCTILLKGPDTIIANAESAALHGAFYDRAAPWLATAGAGDTLAGFITGLLARDDDPFFAATAATWLHADCARDFGPGLIAEDLPERR"
  },
  {
    "reference": "MHLTVFLLKGIVGFLWSCWVLVGYAKGGLGDNHVHSSFIYRRLRNHERREIQREILSILGLPHRPRPFSPGKQASSAPLFMLDLYNAMTNEENPEESEYSVRASLAEETRGARKGYPASPNGYPRRIQLSRTTPLTTQSPPLASLHDTNFLNDADMVMSFVNLVERDKDFSHQRRHYKEFRFDLTQIPHGEAVTAAEFRIYKDRSNNRFENETIKISIYQIIKEYTNRDADLFLLDTRKAQALDVGWLVFDITVTSNHWVINPQNNLGLQLCAETGDGRSINVKSAGLVGRQGPQSKQPFMVAFFKASEVLLRSVRAANKRKNQNRNKSSSHQDSSRMSSVGDYNTSEQKQACKKHELYVSFRDLGWQDWIIAPEGYAAFYCDGECSFPLNAHMNATNHAIVQTLVHLMFPDHVPKPCCAPTKLNAISVLYFDDSSNVILKKYRNMVVRSCGCH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes; Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2.2. Incorporate a signal peptide in the protein design.3. The designed protein should be optimized for growth factor activity activity in order to improve heart trabecula morphogenesis, negative regulation of cell population proliferation.4. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "LKRRQPKKTNELPHPNKLPGIFDDGHGSRGREVCRRHELYVSFRDLGWLDWVIAPQGYSAYYCEGECAFPLDSCMNATNHAILQSLVHLMKPDVVPKACCAPTKLSATSVLYYDSSNNVILRKHRNMVVKACGCR",
    "response#2": "LKRRQPKKTNELPHPNKLPGIFDDGHGSRGREVCRRHELYVSFRDLGWLDWVIAPQGYSAYYCEGECAFPLDSCMNATNHAILQSLVHLMKPDVVPKACCAPTKLSATSVLYYDSSNNVILRKHRNMVVKACGCM",
    "response#3": "LKRRQPKKTNELPHPNKLPGIFDDGHGSRGREVCRRHELYVSFRDLGWLDWVIAPQGYSAYYCEGECAFPLDSCMNATNHAILQSLVHLMKPDVVPKACCAPTKLSATSVLYYDSSNNVILRKHRNMVVKACGCS"
  },
  {
    "reference": "MAPPRSSSGEPLENGAAVKDTDSESKPLTQLLSLVPGAARSAIGNLVAAVGQPTYRVDQIFDWMYARRARSFEEMTNLPSDLRERLIDEFTLTPACRSFEARSKDGTIKHLWRLTDDEEVESVLIPTPGRVTLCLSSQVGCALGCRFCATGDFGFRRHLDTGEIVAQFRDADRIAREVYGRPIGNLVYMGMGEPFANQEPVFESLEVIHYGFGLGARRITVSTVGIIPGILALAELAEPYRLAVSLHAPNHELRLRLVPIEQRYPIDELFAAVDEYQSRKGRRVTFEYTLIEGLNDAKDTARELVDLIGRRLVFVNLIPWNPIPSRNWRPSTASAARRFLSVLEDAGVPAAIRTPRGRDIAAACGQLRLERDLIGEPAPTAAPSRGQESA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have metal ion binding, rRNA (adenine-C2-)-methyltransferase activity to facilitate rRNA base methylation.2. The protein should be able to bind [4Fe-4S] cluster ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "SSQVGCTLTCTFCHTGTQKLVRNLTPAEIVGQIMIARDDLGEWPVQGAPKDETRLLSNIVLMGMGEPLYNFENVRDAMKIAMDDQGISLSRRRITLSTSGVVPEIARTAKEIGCQLAVSFHATTDAVRDVLVPINKRWNIDALLDALREYPKVSNSERITFEYVMLDGVNDSDEDARRH",
    "response#2": "SSQVGCKMNCLFCQTGKQGFEGNLTAGDIINQIYSLPERERLTNVVFMGQGEPMDNLDNVLRATRILTSAYGYAWSPKRITVSSVGVKNKLRRFLDESDCHVAISMHSPLHELRLSLMPAERQMPIADVVELLRQYDFTGQRRVSFEYIVFDGVNDR",
    "response#3": "DKERGTLCVSSQVGCKMNCLFCQTGKQGFEGNLTAGDIINQIYSLPERERLTNVVFMGQGEPMDNLDNVLRATRILTSAYGYAWSPKRITVSSVGVKNKLRRFLDESDCHVAISMHSPLHELRLSLMPAERQMPIADVVELLRQYDFTGQRRVSFEYIVFDGVNDTQAHGREIVRLLDGLECRMNLIRFHAIPDVALRTTDDRRMEALRDYLTSHGVFTTIRASRGQR"
  },
  {
    "reference": "MENIKLDRIEDAVEDFRNGRFVIVVDDEDRENEGDLIIAAEKVTPEHVNFMITNARGELCAPLSISRCEQLGLPHQVNDNTSMLGTPFTITVDKLQGCTTGVSAHDRAATIRALADPESRPEDFGRPGHIHPLYAQDAGVLRRAGHTEAAIDLARLAGLQPAASLIEILNEDGTMARLPQLRKKADEWGLRLVSIKDLISYRLKQESLVEEGVEVDLPTEWGHFRLIPFCQKSNGLEHIALIKGDIANSADPVLVRVHSSCATGDIFGSMRCDCGDQLHKAMQMIDKAGIGAIVYLNQEGRGIGLMDKIRAYKLQEEGLDTVDANLHLGHKADERDYGVGAQILRLLGIQKMRLLTNNPVKRIGLEGYGLEVTENVPIEVAPNPYNRRYMETKKQRMGHNLDKVN",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have zinc ion binding, GTP binding, GTP cyclohydrolase II activity, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, manganese ion binding to facilitate riboflavin biosynthetic process.2. The designed protein should contain one or more GTP cyclohydrolase II domains that are essential for its function.3. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..4. A protein that is capable of catalyzing reactions within the riboflavin biosynthesis, Cofactor biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway with high efficiency and specificity.5. The protein requires Zn(2+) to activate a critical catalytic site.6. We are looking for a protein with broad substrate specificity for GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.7. The protein should have a high affinity for GTP, Mg(2+). The designed protein sequence is ",
    "response#1": "VSVQMPTKYGNFELIAFKQVNTGEIHMALKKGEWEKGEPVLVRVHSSCVTGDILHSLRCDCGEQLAAAMKMVEKEGKGLILYMNQEGRGIGLMNKLKAYKLQEEGRDTVEANLELGFQMDERDYGVGAQILRHLNICKLRLITNNPRKRAGLLGYGLEVVENVSTRIPLQNKGDFNMTVFANELDCAEHFALVKPPAFANRVPLVRIHSECITGDVFGSCKCDCGKQLEQSLSLIAAEGGILIYLRQEGRGIGLANKLKAYALQEQGLDTVDANHQLGLPVDGRDYAIAYQILKYFGIDAIRLLTNNPSKIAAVDRYGIKVMERVPYIEEPPVKLPTEHGEFEFIPFRQTSNGLEHAALVKGKWTRDEPVLVRLHSSCFTGDIFGSLRCDCGSQLHKAMEMVEAAGKGVVIYLSQEGRGIGLFNKIKAYKLQDQGMDTVQANIKLGFGEDERDYGVGASIMRELGLGKIRLISNNPVKRAGLEGYGIKIVEAVPYIDEPPVVEEREVEINLPTSYGDFRTIAYTNQIDGREHIALVKGTISPEEPTLVRVHSECLTGDVFGSHRCDCGPQLESALQQIEEAGKGVLLYMRQEGRGIGLLNKLKAYKLQEEGYDTVEANEKLGYAADLRDYGIGAQILEDLGVRKMKLLTNNPRKIKGLHGYDLELVDRVPYIEEPPVVEEPIVEEPTLEEPTVEEPVEEE",
    "response#2": "MPPERIATTRLPTMHGDFRAYGYRDLVGGGEHLALVKGELSGDDPVLARIHSECLTGDAFGSLRCDCGPQLQAALAAVAKAGRGVVVYLRGHEGRGIGLAHKLQAYELQDNGHDTVDANIELGLPADARDYGVGAQILADLGVRSLALMTNNPAKLTGIEGYGLDVAERDADGSPRERIATTRLPTMHGDFRAYGYRDLVGGGEHLALVKGELSGDDPVLARIHSECLTGDAFGSLRCDCGPQLQAALAAVAKAGRGVVVYLRGHEGRGIGLAHKLQAYELQDNGHDTVDANIELGLPADARDYGVGAQILADLGVRSLALMTNNPAKLTGIEGYGLDVAERDPATGEPRTVLERDRVADARIPTEHGTFRAVGYQSSFDEAEHVALVMGDIAADGGEDVLVRVHSECLTGDVFGSLRCDCGPQLDAAMEMVGREGRGVILYMRGHEGRGIGLMHKLQAYQLQDNGADTVDANLELGLPADARDYGLGAQILVDLGVRSMRLLTNNPAKRVGLDGYGLEIVERSADGTRRVLARDRGAETSLHTPYGDFRAIAFVNDIDDYEHMALVKGDIGPDMDILVRVHSGCLTGDVFGSYRCDCGEQLKRAMSMVQEEGRGVILYLQQEGRGIGLANKLKAYALQDRGYDTVEANEELGFAADLRDYGVGAQILVSLGVRKMKLITNNPKKIVGLEGYGLKVAGRVRTERGADIDAACGQLRTEAERAAATARLRASADTADRPRADTPRADTPRAD",
    "response#3": "VSVQMPTKYGNFELIAFKQVNTGEIHMALKKGEWEKGEPVLVRVHSSCVTGDILHSLRCDCGEQLAAAMKMVEKEGKGLILYMNQEGRGIGLMNKLKAYKLQEEGRDTVEANLELGFQMDERDYGVGAQILRHLNICKLRLITNNPRKRAGLLGYGLEVVENVQPREIYRTKRLERVSSVHLPTIYGDFMAHAYRFILDESPERLHIALVKGDLTAETPVLVRVHSECLTGEVFGSLKCDCGQQLHKAMELIEKEGMGVLLYMRQEGRGIGLLPKLKAYELQEKGLDTVEANLALGYPPDLRDYGVGAQILVHLGLRKIRLMTNNPKKIVGLRGYGLEVVERVREVEINLPTSYGDFRTIAYTNQIDGREHIALVKGTISPEEPTLVRVHSECLTGDVFGSHRCDCGPQLESALQQIEEAGKGVLLYMRQEGRGIGLLNKLKAYKLQEEGYDTVEANEKLGYAADLRDYGIGAQILEDLGVRKMKLLTNNPRKIKGLHGYDLELVDRVPSRGDAAAREAEAKLPSAFGDFRIVAYTNELDGKEHVAIVKGEIDSSKPVLVRVHSECLTGDIFGSHRCDCGPQLHAALRMIEAEGRGIVLYMRQEGRGIGLINKLKAYRLQEQGYDTVEANKKLGFPADLRDYGIGAQILLDLGVKQMRLMTNNPRKIRGLSGYGMQVAERVERVSSVHLPTIYGDFMAHAYRFILDESPERLHIALVKGDLTAETPVLVRVHSECLTGEVFGSLKCDCGQQLHKAMELIEKEGMGVLLYMRQEGRGIGLLPKLKAYELQEKGLDTVEANLALGYPPDLRDYGVGAQILVHLGLRKIRLMTNNPKKIVGLRGYGLEVVERV"
  },
  {
    "reference": "MLRRLLQPAYNVALSGTSASTLPRKSASVGLVRTALMPVDYNAGALFCAMRFTSGTEKERKREPKRGSKRRSKATTTLSTPTDAQTSVTGAREGVALPQENMPDAIQPPATLEVRAAEVKEEPLAATPPQIQPQEETMGPIDGEPQLPLHSTLVYNALTGRMTSETSPSFLCLKSIGFGVNNKRQLYVEKPDVLRDLAQRMRKGTATLPSNWPVTIIRALGVILRNRRVEDPAEAIQQMMQVKINNLTHSRYAAVVNAVGDTDLQNILDGGLADEFVAVDLNEEQEKVINLALKGHLMYIGGSAGTGKTVLLRALCRRMQAEGLRVAMTATTGVAGCHIGGSTFHHAMGVSAQGDFVRKNHLLSYDAIIIDEVSMLPKKMFEEFDRVLREEAGAPDVPFGGVQIILCGDFLQLGVINEPPIIHSTTFREKFVKIRLETQVRQAKSSLFADALQQMRVGLVPESLTASVEQLPPGTMVPAAVNLLPTNKEVNTANEEELKRLPGDAVTLTPETGITALRCDTTATLLMRTTKDFKVEEFTKHLRGLLQATVDIPRASMVSAYRIYEDGHAVRVYLPQSESVAWRDAIRERFLEVAGLINDLDIGATVTEIIPSGDGLHTPEHEECLQRLMAKHPIAQPLTLKKGCRVLLRTNLTSRLVNGSIGTVVDFVECSMENIPVALRCERVNRCVDRYRIYCTMECGMPVPLLPVVKFHSGETIVVPPWEFLVGGNPITQYYSLSSVSLPLSLAYAFTVHKVQGLTLVGRVHLEL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should exhibit Polar residues compositional bias.2. The protein must exhibit the following characteristics: DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of mitochondrial (kinetoplast) genome stability; Involved in the segregation of minicircle progeny..3. A protein that can perform ATP binding, DNA binding, DNA helicase activity in replication fork, nucleus to support DNA duplex unwinding.4. The protein should have a fast turnover rate for ATP + H2O = ADP + H(+) + phosphate. The designed protein sequence is ",
    "response#1": "LSQEQKQILDMVVHQEHNMFFTGAAGTGKSVLLERIITDLRRKYSPGAVAVTASTGLAACHIGGVTLHNFSGIGLGREDVDTLVKKIRRNRKCFKRWKETRVLVIDEISMIDGDLFDKLEQIARRLRTGADKHKPFGGIQVVITGDFFQLPPVPDRKTSGEQKEFKFCFEAEYWPEVITQNYVLKQVFRQKDNTFSTMLNEMRRGDMSDEVVVTSRELDSLALSQSTGLPTLSLPRGTVCLPPALLPYLEQFRRIVFWLGDDLRSWEAAKLFARKLNPKRCSLVRVHRRSVFLTGGAGTGKSHLLRAIIAALPLSTTFVTATTGLAALNLGGTTLHSFSGCGFVDQHTSTHQMVYRNVLGRKKARANWRKCRVLVVDEVSMLDAWFFDMLEYVARHIRGCRKPFGGIQLVLSGDFLQLPPVNKHSPKQETRLCFEAKSWPRVNPLVCTLSHQFRQKDKEFFSLLNEVRVGALTAPSLGLLSSLSVITTVSFVDEEKLKLKREVGAEAVDIITDSKGRLCPPFSLSNYFLLRNGEFSTALIRELERLKFTRLTTRSNLIREQDLHLALLRASLGTSAQHDPSLLRLRFTLPTGPLRPLLPTLPKAADITASAEMEATPAFDDLLLGTPLLLSYTVSWPLDLFLTPTDLALYAALFGYLSALRRTHARVHTAWASLSNAQRARRKWTGLGEGGTREDLESRERLLRCGWGVVRCMGWFLDCLLGYTMSDVVDAEYLKLKSAVAGAPSTKGQGQSNDAKRRARASSVASVAQKSTISKLDSAEPGEALDFTTLRALHSAYLERLLAGSLLGNAALAAVLRPIFELCDAFVAHVERWGGDVLPALLAEGSVSGGGDGVGKMVKERWEIVSEINEALNTLLDSFYEQLTQSTAQPIRPDISRSVFNATAANMTSAFKIGNTTTMRRDARLRENEGETRRQIERLLLRLDFN",
    "response#2": "RGYNTYIGGGAGTGKSLLLRVIRQELVSRGLTVATTATTGIAARRLNGATLHHCFGVNVYGEFTRRAELKEFDVIIIDEVSMLSKELFESLEFQLRRANGVDLPFGGVQVILSGDFLQLGAICSVSLVHSSVFRRNFAMLKLR",
    "response#3": "LSQEQKQILDMVVHQEHNMFFTGAAGTGKSVLLERIITDLRRKYSPGAVAVTASTGLAACHIGGVTLHNFSGIGLGREDVDTLVKKIRRNRKCFKRWKETRVLVIDEISMIDGDLFDKLEQIARRLRTGADKHKPFGGIQVVITGDFFQLPPVPDRKTSGEQKEFKFCFEAEYWPEVITQNYVLKQVFRQKDNTFSTMLNEMRRGDMSDEVVVTSRELDSLALSQSTGLPTLSLPRGTVCLPPALLPYLEQFRRIVFWLGDDLRSWEAAKLFARKLNPKRCSLVRPVEQAAGVRWSRFPDLNRLLKGHRKGELTVFTGPTGSGKTTFISEYALDLCTQGVNTLWGSFEISNVRLARVMLTQFAVTRLEEQLDKYEEWADRFEDLPLYFMTFHGQQSIRSVIDTMQHAVYVYDVCHVVIDNLQFMMGHEQLSSDRIAAQDYIVGAFRKFATDNSCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKSC"
  },
  {
    "reference": "MSKQLVGLVGWRGMVGSVLMERMQAEGDFELIEPVFFSTSNAGGKAPAMAKAHKELHDANDVAALTKCDIIISCQGGDYTKEVFPKVRASGWQGHWIDAASALRMQDDAVIILDPVNDDLIQARLAAGGKNWIGGNCTNSILLMGLVGLFKAGLVEWVSSMTYQAASGGGANHMRELLKGMGVIHGAVASELATPASAILDIDRKVAQTIRQDVPREFFGAPLAGGLIPWIDAQLENGQSKEEWKGKAEVNKILGTPTPIPVDGLCVRIGAMRCHSLALTLKLKKDVPLAEIETLIKGGNPWVKFVPNEREQTVRELTPAAITGGLQIAVGRVRKLNIGPEYVSAFVVGDQLLWGAAEPLRRMLRILLKA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Acyl-thioester intermediate active site of the designed protein must be optimized for substrate binding and specificity.2. The protein should be optimized for activity in L-threonine biosynthesis, Amino-acid biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The designed protein must have at least one Semialdehyde dehydrogenase NAD-binding domain.4. The designed protein must be able to regulate diaminopimelate biosynthetic process.5. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate. The designed protein sequence is ",
    "response#1": "LEPVRKKFGLKKIIVSTYQAVSGAGAAAIEELREETKANSEGKSYEPKVLPVKSGEKHYQIAFNAIPQIDNFEDNGFTFEELKMINETKKIMHDTGLQVAATCVRLPVETGHSESVYFEIDSEGVTAGEMKELLKDAPGVVLQDDPANQIYPMPVNSVGKHEVFVGRIRKDLDNDKGFHMWVVSDNLLAEPDGSTVIHGLFERDMIEWASVMTYQAASGAGAKNMRELLQQMGETHAVAKDLLDDPASSILDIDRKVVEKMRDEDFPAQNFGVPLAGSLIPWIDREVTHGQSREEWKGQAETNKILGHADHPVPIDGICVRIGTMRCHSQALTVKLKKDVPLEEIEKIIAASNQWVHVIPNEKEASAKELTPVAVTGKLDIHVGRIRKLNIGNEYISIFTVGDQLLEIQ",
    "response#2": "MGLFREGLVEWVSAMTYQAASGAGAKNMRELLEQMGALHEAVGARLDDPASAILELDRAVTAAMRAEDFPCANFGVPLAGSLIPWIDAQMPNGQSREEWKGQVETNKILGREEQPIPIDGICVRVGAMRCHSQGLTIKLNRDLPVVDAEAIIAEANPWVKLIANDRDETIHHLSPTAVAGSLTVPVGRLRRLAMGDRFLAAFTVGDQLLSDNL",
    "response#3": "LEPLKAKFGLRRIIVSTYQAVSGAGNEACDELANQSKTYLSNEEMKATILPVKGDEKHFPIAFNALPQIDLFQENGYTFEELKMINETKKIMHAPDLSVAATCVRLPFFTSHAESVYIEVEREDVMVKDIWEVLQHAEGVVLQDNPKEQIYPTPLAAEGKQDVFVGRVRKDLDNRHGFHLWIVSDNLLKHGLDAGLFREGLVEWVSAMTYQAASGAGAKNMRELLEQMGALHEAVGARLDDPASAILELDRAVTAAMRAEDFPCANFGVPLAGSLIPWIDAQMPNGQSREEWKGQVETNKILGREEQPIPIDGICVRVGAMRCHSQGLTIKLNRDLPVVDAEAIIAEANPWVKLIANDRDETIHHLSPTAVAGSLTVPVGRLRRLAMGDRFLAAFTVGDQLLAA"
  },
  {
    "reference": "MKILTAEQIRKIDSETIEREGISSLTLMKRAATAFTNFLMTRCPDKKQEILIFAGTGNNGGDALVVARLLHQSGYRVKVCVVDQGSRYSEDCAHNLRRAKGEHIPCKIITGEQDIPSMERFDLLIDGIFGTGLSREVEGVTAEVIEAINKSGKPVYSIDIPSGLFMDRSTRLAVRATVTVTFQIPKLALFLPENGDYTGELQIVDIGLNAGAIAEAGSHMEYAVKETVAPLLKPLHRFAHKGTQGHTLLVGGSLGKCGSLCLAAKAALRTGCGLVTSFLPRCGTTVMQSVVPEAMVLEDPDVEHITEISYAIEPDAVGIGPGMGQHEETAQALAAFLTGYRGALVIDADGLNILSRHPEWFSLLVPGTILTPHPKELERMIGSWRDDFEKIEKARLFARRYGVVIVVKGAYSLIIDSDHLYVNSSGTPALATAGSGDVLTGMIASLLAQGYKPMDAARVGVYLHGLTADLTQKTIDPRSFMAGDIISHIGAAYQSIEKKE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration2. The designed protein must be able to perform ATP binding under specific conditions.3. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "LIIGGSTSMPGAPSMAISACLYSGAGLLTAALPESILTSVSVNNPEVMLLPLPEKKGYIAPEIISTDFTFDSYDAVAAGPGLGRYQGTKAVVKKLLQGVKAPLVLDADALYYVPELEQEIKERQVPLILTPHPGEMARIAGVSVHEVNKNRFSLSRAFAVQHGCYLVLKGPSTIVTTPNGDQFINTTGNEALARGGTGDILTGMILGLLLQKGDVSSSISNAVYLHGAAADLAVKHEFHSYSMVSTNLIRFQ",
    "response#2": "TPEMVRDWLPPLEATTHKGARGTVLVVAGSIGFTGAAVLTSEGALRAGAGLVFLACPKSLNPIYEVKLTEVITRPVPEPDVAGCFGPESVDTLLQEVSRADALAVGPGLSTAQPASDMLAAFLPKVQVPTVLDADGLNILAARPEITLPPECVLTPHPGEMGRLMGMAAKEVQESRIRIAREAAYRFERIVVLKGGATVVAEPAGEAYINPTGNSGMATGGTGDVLTGAIAALMARGMAPIKAAVAGVYLHGLAGDLVAEELGREGMAAGDLLLKLPLAFRRRITARELRQWLQPRRACAHKGEHGRLLLLGGDHGFGGAIRLAAEAGLRSGAGLVRVLTHMEHIGPLLTACPELMVQRLSDDTLQQALEWADVVVVGPGLGQGDWGRNALKQLQSSDKPAVWDADALNLLALHPEKRQNRVITPHPGEAARLLNCRTADIESDRLLAVRRLVAQYGGVAVLKGAGTLIADQQGDMAIADVGNAGMASGGMGDVLSGIIGGLLAQKLSLYDAACAGCVVHGAAADRVAEEQGTRGLLATDLLPMIPYYR",
    "response#3": "LIIGGSTSMPGAPSMAISACLYSGAGLLTAALPESILTSVSVNNPEVMLLPLPEKKGYIAPEIISTDFTFDSYDAVAAGPGLGRYQGTKAVVKKLLQGVKAPLVLDADALYYVPELEQEIKERQVPLILTPHPGEMARIAGVSVHEVNKNRFSLSRAFAVQHGCYLVLKGPSTIVTTPNGDQFINTTGNEALARGGTGDILTGMILGLLLQKGDVSSSISNAVYLHGAAADLAVKHEFHSYSMVSTNLIRFD"
  },
  {
    "reference": "MIPARYAAYCPNCGGEVESERLAAGLACARCQPDPAKPPLPGALARFFAQEEALADWARFFAAHVGAPPWPLQRAWAARVVQGRSFAMLAPTGIGKTTFGLLTALWLAREGRRSYLVFPTRLLVAQAAERLRALGAPFVAYTGKPKEKEALLEGERPIGLFTVAFLYKNHARLPRPVDFLFVDDVDSLLKSARNVDRVLELLGFAPEDLEKGLSLIRLRRKNPEEAERMAEGLRRKARGVLAVASATARPRSPRVHLFRELLGFEVGTPSFALRKVADLYEEAFGLSQEALWARGAEWARRLGGGGLLFLPGDLPKEAALDLARFLRARGLRAKAYLEPEALEAFRRGEADHLVGFASWRNPLARGLDLPGRVRYALFLGVPKLRFALGEDLAPGELVRLGLTLLPLLEDPGLKALVRSLARRPGLQEKEVAPLRQALLERLADPVFRARVAESPEVGLTFQGEKPVLVFADVTGYLQASGRTSRLTPAGLTQGLALLLAEDRKAFTALTRRLGYLLEAPPRPVAEVDLEALLKRVDEDRDRLARGERGGLALPERVVVVESPNKARTLAGFFGRPMRRYLPGLVVYEALSEEGYLVVTATRGHVTDLALRGGLYGVETAPAYRPRYHPLRACPEGSVPDPFCPDGRASRPDRDRALEALRSLALEAQDFLLATDPDTEGEKIAWDVLLALRAFGKEVARGEFHAVTPRAFLEALRRPREVKEDLVEAQMVRRIADRWIGFALSEDLQRLLRRRSLSAGRVQTPVLGW",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: May be involved in rewinding the DNA strands in the regions of the chromosome that have opened up to allow transcription or replication..2. The designed protein should be optimized for helicase activity, hydrolase activity, DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity activity in order to improve DNA unwinding involved in DNA replication.3. The protein contains novel Toprim domains that confer a unique function or activity.4. The For DNA cleavage activity active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "MNLVNAGEARRILDRLYGYTISPLLWKKVAYGLSAGRVQSVGLKLLIEKEKTRINFKKANYYSILLQCKHEKKNLLLEAKLEEIDGKNIAEGKDFVNETGKLKNIAKTTIITQDLMIELEKELKNGQKIELISIETKKIKIPPPKPFTTSTLQQEINKRLKIGTKQIMQHAQKLYEHGYITYMRTDSHNIAKIAKDKITKIIKNKYGKEYIEEKDRIYEKEKMAQNAHEAIRPSEIFIPNETIEIESKTAKEIYKIIWDRTIISGMKDAIKENIKLTFKYKNLIFRSSFTKIIFDGFLKHTKEQDEHLNINFDLIKKGDTFSIVKMKTSEHETKAPFRYTEASLVQKMEKEGIGRPSTYSTIISTLLEREYAFKLNNTLMPTIKGAAVINLLEKYFPVLIELNFTSNMEEKLDKIAIGKLDKIKYLSKFYNGKKGPVKRLWISSVTDKAIKDGFKQLKDGKRYETLYAAAAARAEADWYVGINGTRALTTKHNAQLSCGRVQTPTLAILADREEAIRKFVPKPYHR",
    "response#2": "VGQRMWSLQETWARRILMGSSFSIVAPTGVGKTVLGTVSALYFSTKGKRSYIIVPTALLVQQVSDRLSTFSDRLGIKPKIVCYHAGMSGSEKRRALERVARGDFEILVTTERFLIDRFDVIEGLAFDFIFVDDVDSFLRSSKNIDKVMGLLGFSNEMIASASRIIELRREASRLEKMGRPTDQISAEIEMLRSRIRDYRRQNRIGLLVVSGATVKARRTKRIKLFEELLDFQIGFKPEFLRNIKDEIRRMEFHEVTKKAILNSLKEYRDVDENLVKAQVVRRISDRWVGFELSQLIQRIFYKNYLSAGREPVLLIVESPNKARTIAGFFGKPMSRRFGEFEVLEVAAGDLYLMITSSLGHILDLAKEGGFHGVLTENGEFVPLYEVLEEKRETVKGLRQIGKEIESLYVATDPDTEGEKIGWDISAVLAPYVPVKRLWISSVTDKAIKDGFKQLKDGKRYETLYAAAAARAEADWYVGINGTRALTTKHNAQLSCGRVQTPTLAILADREEAIRKFVPKPYHA",
    "response#3": "TGSRPWSVQRQWARRLLRRESFAITAPTGTGKTTLLAVYALYSAVNGSRVYYLLPTSSLAKQVAERLQLLAHNAAVRKRIVYYHSLLRASEKRAQLEAIENGEYDILVTTTAFLSRRWGLLERTMFDTIIVDDVDAILRDSVNVERILMLLGAKPDEIRAATRLVKLSLRVRANPALARRYASEIEELKRVVNGVAGRLGQLVVASATGRARGFKRLVLGKLLGLSVGSIAGYARNIAENYKRMIFHEITETAITESLKNTRNIDMNLVNAGEARRILDRLYGYTISPLLWKKVAYGLSAGRVQSVGLKLLIEKEKTRINFKKANYYGDWYVGINGTRALTTKHNAQLSCGRVQTPTLAILADREEAIRKFVPKPYHTISVETKDGVTFRWADRRSKEERIFDDKRAKTLQEKLQQSAIVIDSVNAKTKRTPPPPLYDLTELQREANKRFGYSAKETLAALQRLYEQHKAVTYPRTDSRYLSSDLVETLKERLHAVDVQPFRKMVANAKKQGLANAKRQMVNDAKVSDHHALIPTEQPQSASAMSDKEAKLYHLIVRRFLANFFPPSESELTSVEATANGELLRAKGERLVSAGWRGQETGEEDEALARLLPAMSKGDKKAVKWVGMTQGKTSPPPRFNEGTLLAAMEKPAQHMEAKDETIKKTLAQAGGIGTVATRADIIEKLFSSFYIEKKGKDLYITSKGKQLLELVPAELKSPALTAEWEQRLEKIVQGKEQKQMFIAEMKEYAKAVVKRLLKGRSFSIIAPTGMGKSTFGAFMSLWHAKRGKKSYIIVPTTPLVIQTVKKIEAMRERLGLNVKLAYYHGNLRKKEKEEMLALIDSGEYDILVTSAQWLARNFEDKLKGRHFDFIFVDDVDAFLKASKNIDRSLYLLGFNEETIQKAWEIVRLKKGMARYLNGNSEDKNEKLKELNEQIEKIQREIESYKRKNRIGVMIIASATGSARGDRIKLYRELLGFE"
  },
  {
    "reference": "MVVKLYGQVTAACPQRVLLCFLEKGIEFEIIHIDLDTFEQKKPEHLLRQPFGQVPAIEDGDFKLFESRAIARYYATKFADQGTNLLGKSLEHRAIVDQWADVETYYFNVLAQPLVINLIIKPRLGEKCDVVLVEDLKVKLGVVLDIYNNRLSSNRFLAGEEFTMADLTHMPAMGYLMSITDINQMVKARGSFNRWWEEISDRPSWKKLMVLAGH",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Functions in the cytosol to maintain the regular accumulation in the vacuole of PA precursors, such as epicatechin and glycosylated epicatechin.; Involved in the transport and/or accumulation of both anthocyanins and proanthocyanidins (PA)s in the vacuole2. A protein that can perform cation binding, glutathione binding, glutathione transferase activity in nucleus, plant-type vacuole membrane, cytoplasm, cytosol to support regulation of flavonol biosynthetic process, toxin catabolic process, anthocyanin-containing compound metabolic process.3. The designed protein should contain one or more GST N-terminal, GST C-terminal domains that are essential for its function.4. The desired protein should be able to perform glutathione + RX = a halide anion + an S-substituted   glutathione + H(+) The designed protein sequence is ",
    "response#1": "AVVLLIAQEKERNIFDQRAIENELLDRKIHVIRRRFEDVSERGSLDQNRRLFMEDQEVAVVYFRDGYMPSQYNAQNWEARLLLERSCAAKCPDIATQLD",
    "response#2": "AVVLLIAQEKERNIFDQRAIENELLDRKIHVIRRRFEDVSERGSLDQNRRLFMEDQEVAVVYFRDGYMPSQYNAQNWEARLLLERSCAAKCPDIATQLS",
    "response#3": "VPSVIFSHPEAGSIGLSCKEAKEKYGEDQIKIYKSKFNAMYYAMMEDDSLKSPTSYKVVCAGEDEKVVGLHIVGDSSAEILQGFGVAIKMGATKKDFDSCVAIHPTSAEELVTYS"
  },
  {
    "reference": "MVMLDTLANALATIQNAEMRAKSEALIWPASKLIINVLRVMQREGYIGEFEYIDDGRWGKIKVQLLGRINKTGVIKPRFPVKLRDLERMPHWLRKYLPAYNIGILILSTPHGVLSHKEAIAKKTGGVLLAYVY",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit..2. The protein design must be able to perform structural constituent of ribosome, rRNA binding in order to contribute to translation. The designed protein sequence is ",
    "response#1": "RKKSAKVREVLADPIFNSVVVTKLINTIMLDGKKSIAQDILYSAFDLVKEKTKKDPMQVFTQAVENITPQLEIRTRRIGGTNYQVPTEVSKRRKQALSLRWLVQYARLRNDKSMDLRLANEIIDAANKTGGAIKKREDTHKMAEANF",
    "response#2": "PRKGNVPKREVLADPIHGSMVVAKLINSVMLDGKKGTAQNIVYGAFDIIKERAEENPVEVFEKAMNNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLRWLVKYTRARGEKGMIEKLAKEIMDAANNTGSSVKKKEDTHKMAEANF",
    "response#3": "PRKGNVPKREVLADPIHGSMVVAKLINSVMLDGKKGTAQNIVYGAFDIIKERAEENPVEVFEKAMNNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLRWLVKYTRARGEKGMIEKLAKEIMDAANNTGSSVKKKEDTHKMAEANF"
  },
  {
    "reference": "MKKNNERVNTNPSLISKSYNMKKRPVIGTRRHFARKEIESDSDDEDDIFLAGERKKYLQNQSYERRKQKNEHGNNLTLQEELLRHEAYEKNEEIANENDVDQVNLMKYFVHMDNISPVTTNGSLKESLRKYSSIIAVNIFSETGDLKKGIAIFQDLSDAEQAVQYLSNCKIDGRLISATITNHPKRLPNAEHLESSTKTKDESQDKDKLTKLDRAKLEWLIQGLNCEKGSIGNLLCFAVEHVNNHVEITDAFLKEFFNDQPTDDTKVYDDDYINERGEKKLSLIYLMNDILFNGISGTSLVWRYRFSFEPHVERLLDDLYLFSKRLGGRIKEDIFCKKVVKVIEVWKTWIAFQEETLERAWRNFSGNTPQSPQINSAALKVETKNSWTAISEETEGLQDDEEYNGIPVDVNELLNVEFISQIPMSTETSSSSSPQPTEERKAKFKPSFTQGTFVSKRMRMHAEDLF",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a specific function within the nucleus, U2 snRNP, cytosol.2. The protein should exhibit Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "FTFKWPKGPEAIILTGTFDDWKGTLPMVKDPSGAFEITLPVTFDSPSSKFYFKFIVDGQWLPSKDYKVNIDEGVENNFIL",
    "response#2": "PVPVKQTFSRFDSTSPVNDPENRFKYFTNAVLSPLVRSSAGGRGNPSASGTLIFIPSYLDFVRVRNYLATSSQTENLSFGAISEYTSVRDVARARTHFFSGRHSALLYTERTHHFRRYQIRGVKRVIMYGVPENPVFWAEIVGFLGLDPAGAAEAAEGGVRALFSKWDALKMERIVGTKRLGNMLIEKGGDTFTI",
    "response#3": "FTFKWPKGPEAIILTGTFDDWKGTLPMVKDPSGAFEITLPVTFDSPSSKFYFKFIVDGQWLPSKDYKVNIDEGVENNFIE"
  },
  {
    "reference": "MRKKQILLWASAAAGVVALFFAGRSAVARGRANAATLTNNETGTKTICFAGGCFWGTEHFFKQIRGVVSTEVGYANGKTDTAPTYEEVSTETTGFVETVRVTYDPAVITLDQLIQLYFQTIDPTSLNRQGNDQGTRYRTGIYYTDEADAETIHRAIAALAVKLGKPVAVEEGPLRNFYPAEEYHQDYLVKHPTGYCHISPALFEMARKANPAPRYSRKDDATLRRTLTEEQYDVTQNSATERPFTNAYCGEFRPGIYVDITTGEPLFVSTDKFESGCGWPAFSKPIDKSLITEHADHSHGMERTEVRSKTGNAHLGHVFTDGPKDRGGLRYCINSASLRFIPRDRMEAEGYGEYLKLVPEAEAEKKE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The presence of MsrB domains is critical for the interaction of the designed protein with its intended targets.2. The active site, Nucleophile active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. A protein with L-methionine:thioredoxin-disulfide S-oxidoreductase activity, peptide-methionine (S)-S-oxide reductase activity, peptide-methionine (R)-S-oxide reductase activity is needed to enhance response to oxidative stress, protein modification process in a specific context.4. For general function, the protein need meet that Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. The designed protein sequence is ",
    "response#1": "METAVIGGGCFWCVEAVLRELNGVASIRPGYAGGHTANPTYKEVCTGTTFHAEVVEVTFDPAILSYVDLLRIFLTIHDPTTPNRQGADVGTQYRSVIMPASTEQENAARGVLAEVEAAQIWQAPLSTTIEPLQIFYPAEPEHVDYFARHPEAAYCQAVISPQHGDTTQFHKPSDEEIKKMLTAEEYAVTQKNDTERAFSNRYWDKFDEGIYVDIVTGEPLFSSKDKFDSGCGWPSFSKPISPDVATYKDDHSFNMVRTEVRSRVGDSHLGHVFTDGPKDKGGLRYCINSLSITFIPEEATPENPFF",
    "response#2": "LTAAQYEVTQKSATEPPFQNEFWNTFQPGIYVDITTGEPLFTSSDKFESGCGWPSFSKPIDPDVIHEKKDTSHGMQRTEVRSRAGNAHLGHVFNDGPKKTGGLRYCINSASLRFIPEAGFANRLHTLQTAKGKPVSALLQADKPTLVKFWASWCPLCLAELEHTARWQQSPEFAQANLITVASPGFLGEKPQPDFGQWFGKLDYPGLQVALDDGTLAKQAGIGVYPSWVLLSPQGDILRVHKGSLNQTQAQALLTQPDAA",
    "response#3": "PEAGFANRLHTLQTAKGKPVSALLQADKPTLVKFWASWCPLCLAELEHTARWQQSPEFAQANLITVASPGFLGEKPQPDFGQWFGKLDYPGLQVALDDGTLAKQAGIGVYPSWVLLSPQGDILRVHKGSLNQTQAQALLTQPDR"
  },
  {
    "reference": "MRTRTNPDPLKSLDNFPGDRNGGFLLRFLTCGSVDDGKSTLIGRLLYDTNSVFDDQLLVLKQESIRYGTAENELDFALLLDGLDAEREQGITIDVAYRYFSTAERAFIVADAPGHEEYTRNMITGGSTADLAIVLVDCRHGVRQQTKRHSFIASLLGIRHIVLAINKMDLLQYQRESYDKIVSDYTAFAERLKFASIRPIPMSARYGANVITKSVDMPWYDGPSLLAHLESIPVISHTFEEPFRFPVQWVNRPNLDFRGFAGQIAGGLVSVGDKVTVAESGQQTTIKRIVTYDGDLSSAFVGQAVTLVLNDEIELSRGDMLVSPTARPNVSDQFQAHVIWFSHEPLIPGRPYILRTETDNVSVTITTLKYSIEINTFTHVSAKTLAINEIGVCNLSTQRPICFDNYAENRITGNFILIDRFTKATVGAGMIIHSLHRAHNIHWQALTIDRTARAEQKRQRPAVLWFTGLSGSGKSTIANLVEKLLHAKGKHTFLLDGDNVRHGLNRDLGFTAEDRVENIRRISEVARLMSDAGLIVLVSSISPFLSERQMARDLMCKGEFIEIFVDTPIEECMRRDPKGLYRKATAGQIPNFTGISSPYEKPPNSEIHLPTMGKDPQELAQLVENYLNQIYIRS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must have at least one Tr-type G domain. The designed protein sequence is ",
    "response#1": "RFITCGSVDDGKSTLIGRLLYDSKLIFEDQLAALEADSRRIGTQGQDIDFALLVDGLAAEREQGITIDVAYRFFATEKRKFIVADAPGHEQYTRNMVTGASSADLAVILIDARKGVLTQTRRHSYLVHLLGIRHIVLAVNKMDLIGYAQAQFDAIVADYRRFAGEIGIHDFLAVPISGLKGDNITGRSAATPWYQGPSLLEHLESVPLY",
    "response#2": "PMLRVIACGSVDDGKSTLIGRLLAGTGSATEDQLDYARRTRRGGSTIPAGAVDYSLLTDGLEAEHEQGITIDVAYLFLDLPSGRRVIIADAPGHEQYTRNMAVAASTADVAVLLVDATKGVREQTHRHLAICALMGVRAVIAVVNKLDALDYDQAVFDRLAGEVRAAADGYGVTASVLPASALSGDNVVERSPRLPWYDGPTLLDALATWL",
    "response#3": "LPHVNIGTIGHVDHGKTTLTAAITKVLADKGGAEFKDYANIDNAPEERERGITINTSHVEYKTENRHYAHVDCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLSRQVGVPKIVVFLNKCDMVDDEEMIDLVEMEVRDLLSAYDFDGDGAPVIRGSALGALNGEAKWVAAIEELMAAVDEYIPTPE"
  },
  {
    "reference": "MSAALRFDNIGKVFPGVRALDGISFDVHAGEVHGLMGENGAGKSTLLKILGGEYQPDAGSVLVDGRPVQFANAAASIAAGIAVIHQELQYVPDLTVAENLLLGRLPNAFGWVKKREAKRYVRERLAEMGVDLDPDARLGRLSIAQRQMVEICKALMRNARVIALDEPTSSLSHRETEVLFKLVDDLRAQGRALIYISHRMDEIYRLCDACTIFRDGRKIASHDALADVPRERLVAEMVGREIADIYHYAPRTLGDVRFSAEGVDGPALREPASFSVRAGEIVGFFGLVGAGRSELMRLVYGADRRRAGALTLDGKRIDVKRTGDAIRHGIVLCPEDRKEEGIIAIASVAENINISCRRHSLRAGLFINGKTESETADRFIQRLKIKTPNRRQKIRFLSGGNQQKAILSRWLAEPDLKVVILDEPTRGIDVGAKHEIYDVIYRLAERGCAIVMVSSELPEVLGVSDRIVVMREGRIAGELPRADANEHAVLNLALPQTSAVEAA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a binding site that is specific to the target ATP ligand.2. We are looking for a protein with broad substrate specificity for ATP + H2O + L-arabinose(out) = ADP + H(+) + L-arabinose(in) +   phosphate. The designed protein sequence is ",
    "response#1": "VMAMGTSICHMLLGEKEQEVEGMCGVVEDGIIPGYLGYEAGQSAVGDIFAWFVKHGVSAATFDEAQEKGVNVHALLEEKASQLRPGESGLLALDWWNGNRSILVDTELSGMLLGYTLQTKPEEIYRALLEATAFGTRAIVDAFHGRGVEVHELYACGGLPQKNHLLMQIFADVTNREIKVAASKQTPALGAAMFASVAL",
    "response#2": "LPIPRSLIAHKYEIGHLLLVVGSRKYGGAAILAALGAKATGIGMLSIAVPHSLKSLILAQVPDALVIGCPETESGAIASLPNLDLQKYRAIACGCGLSLDAGQIVEQIINCDRPLILDADGLNSLAAMGEARIIEKLNHRTNAIVLTPHWGEFCRLFPDLHEVERLEALQTAVNQTKATILLKGSKTAICSPQETKSQVWINPESTSALARGGSGDVLAGMIGGIIAQGVAARDAAIIATVWHSQAAMWLASQRTEMGVDPVTLAENLA",
    "response#3": "GQRVIDTFFPITKGGAAAVPGPFGAGKTVVQHQIAKWADVDLVVYVGCGERGNEMTDVLNEFPELIDPTTGESLMNRTILIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERAGQVIALGKDHREGSITAISAVSPSGGDISEPVTQNTLRVVKVFWGLDSQLAQKRHFPSINWLQSYR"
  },
  {
    "reference": "MRIFTSEQTRQAEENAVAMGMNWLRLMENAGAAASRVIREKFSVKNKNVAIICGRGNNGGDGYVVARKLNESGAKVTVIQSNGAPGTPTAFEMYRRITAIGIPVIEYKSNRERAEQIIKSSEIIVDGIFGIGFYGEVAGDNKKLIEIINGTHSKVVSLDVPSGADCDTGAVNGVCVKADLTVSFTAYKPCHVMFPACEYCGEVVAVSIGMPAEAVDSISTSLRSIEMADVLPNLRVKKRNTHKGDFGTALLICGSLGMSGAAAIAAKSAIRTGVGLVKAVVPKSIYCPLASSLYEPVFRVAPETDKGVLSAESLDLIFNELEGSTAVLIGCGLGRDENITQIVKELITKANRPIVLDADGINAICGCIDIIKQAKAPLILTPHPGEMARMLNTTSDKIQAARLKTARDFATEYGVYLVLKGSGTIVALPDGRAFVNLTGNYGMSSAGSGDMLAGMLVSLLAQGLTPEQAAVTSVYLHGHAGDRAAAKLSKRSMSASDMIDELPLLFHEIETAG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein with ATP binding, NADPHX epimerase activity, metal ion binding functions is required to enable nicotinamide nucleotide metabolic process.2. The presence of Mg(2+) is necessary for the protein to function in the desired environment.3. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..4. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.5. The designed protein should have a high specificity for AMP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MVAAEKAADAAGVTYAQMMETAGKAVAEAIMSRRPLAEAKVLVLVGPGNNGGDGLVCGRYLAEAGADVAFYLYKPRDPESDVNYAKILEMGLFTIEAGFDQRYRVLRTRLDITDILVDALLGTGVSRPIGGNLGKLMRLIRDKVTRWRQNDKVTRWQGDKARTEPAEVVTDVSHLVSISSPHPLTPSSPHPLIVAVDCPSGLNCDTGALDPLAFPADLTVTFAGPKRGHFIFPGAAACGELVAADIGIPPDLPQGTTGLPGIAPRRPTIHKGSIGLVLALGGNLGMPGAIRMAAESALRAGAALVAVGCHQDNQAIVVQGRPELMLAPSEPERLVASSYTDKAKVILIGPGLGRDAWANQLVQAAIKAEKPMVVDADALIAMKALGARSDNWVLTPHPGEAAFLLDCDIAAIEADRFAAVRAIAEQFGGVCVLKGAGSLICDGQQVIVNTTGNAGMASGGMGDVLSGIIAALILQTGSLMDATRLAVSIHGQAAEHVTQNTGMRGLLASDLFEPIRQLVNQWL",
    "response#2": "MQEIDRRTILEIGIPGRVLMESAGRGCVAALDRLAGGRALSRVVVVAGRGNNGGDGFVVARYLAERGVEAPVFVVGGTDGISGEAFENLSLLEALGVAVVEVVEEVDLTALTRALSHCDMVVDALFGTGLQREVTGLYAEVVSAVNASKGPVLAVDIPSGVCADTGRVLGCAVTAQATATFCLPKAGHFLYPGWEHCGELTVVDIGIPNRAVEDGHKGDFGDVLFVAGASNYFGAPYFAARSLLKAGGGYARLASIPSVCQVVATKGSEIVFIPLEETKAGSIALANLEKLLELAELVDLVVIGPGLSLQSETQELVRALVRRINKPVLIDGDGLTALAMDIESLPHRENSTILTPHLGEMSRLMEVPIPYIKEHRVEVLRQAAQEWQAFVVLKGAHSLIGYPDGRVFINLSGNSGMASAGSGDVLTGTIAAAYGLGLELGEAVRTGVFLHGLAGDLAAEDKGLDGITAEDILEYL",
    "response#3": "MRNRQQWRRRPCYCQDPEGAECGRDGGGHRRSKKGSPLFFWEMQRLLERGCRMEKEYREEDGDLLVDAMFGVGLSRELSPRDQELIRRLNAMKKEVWAVDVPSGVCASTGKIYGEALKAQVTVTFQAEKPGLFLFPGKQYAGEILCRDIGISLDGVHPEALGEAAPAPSLWQGLMPRPAAQGHKYHRGHVLVLGGARLTGAARLCAQAAQRSGAGLVTVLAPQPVWPVYAQALQASMVQPFADAQQLGALLDDARAQVCAIGPGAGVQALTRQAVLAAAQRGKALVLDADALTVFAQQPQALFQALAANRAATAVLTPHEGEFARLFPDLAGSKPERAAQAARLAQAVVVLKGADTVIAAPDGRVLINSNAPPELATGGTGDVLAGMVAAWLAQGMPALAAACAAVWLHGQAAQRALGREGADAWGRRLTAEDVLHLLDQTPALPEPRLKNSHKGSNGSVGVIGGARYMVGAAILSARAALLVGSGRVYCGLLAPDAPSVDISHPELMLRDAESILELPQLSILLIGPGMGLAGDAESLLARALKLSLPLLLDADALNLISQKPLLKTLLLERQPQSTVITPHPGEAASLLDCSNAQIQSDRLGSASKLTKEFNAMTVLKGCGTVIALPDGRTFINVSGNPGLSSAGMGDTLAGIIGGIFAQGLLLEEAALLGVYLHGAAADALVSQHIGPNGLTASEVALESRRLLNQPLDTHKYRQGSLLLVAGSTTYAGAAVLAGLGSRASGVGMVTLAVPAELKGLVHSALPEVLVHAWEGIPSLLAEKPFQAVAVGPGVGETRRLLLEILLQDWAGIPWVIDADGLNALAEIGVERLPEHRIQAILTPHLGEFRRLFPHLPLSDRMEAAQRAAAQSGAVVVLKGARTVIASPTGECWVNPESTPALARGGSGDVLTGLIGGLVARRRAQLGQSMGIAELLSCACAGVWWHAQAAIHLAGERGLTGVDPAHLAEHLPKAL"
  },
  {
    "reference": "MAATDVDIFSNEEKRNLSLGGHVGFDSLPDQLVSKSVTQGFCFNILCVGETGIGKSTLMNTLFNTMFENEEASHYQNGVYLRPRTYDLQESNVHLKLTIVDTVGFGDQINKEESYKPIVDYIDTQFENYLQEELKVKRSLFNYHDTRIHICLYFISPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADTISKSELHKFKIKIMSELVSNGVQIYQFPTDDEAVTEINSSMNAHLPFAVVGSVEEVKVGNKTVRARQYPWGVVQVENESHCDFVKLREMLIRVNMEDLREQTHARHYELYRRCKLEEMGFKDTDPDSQPFSLQETYEAKRKEFLGDLQKKEEEMRQMFVNKVKETEAELKEKERELHEKFEQLKRMHQEEKRKVEEKRRELEEEMNAFNRRKVAAETLSLSQPLKKDKDKKN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The presence of Septin-type G domains is critical for the interaction of the designed protein with its intended targets.2. The designed protein should be expressed specifically in the microtubule cytoskeleton The designed protein sequence is ",
    "response#1": "QGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVYFKQRITADLLSNGIDVYPQKEFDEDAEDRLVNEKFREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSNIHFEAYRVKRLNEGNSS",
    "response#2": "MNVYFNEAGGNKYVPRAVLIDLEPGTMDALRSGPNGALYRPDNFIYGQSSAGNNWAKGHYTEGAELVDQVIDVVRREAESCDCLQGFQVTHSLGGGTGSGMGTLLISKIREEFPDRMMATFSVMPSPKVSDTVVEPYNATLSVHQLVEHSDETFCLDNDALYDICIRTLKLSSPSYGDLNHLVSAVMSGITVSLRFPS",
    "response#3": "KDVFFYQSDDTRYIPRAILLDLEPRVLNGIQSGPYKNIYNPENFFIGQQGIGAGNNWGAGYAAGEVVQEEVFDMIDREADGSDSLEGFMFLHSIAGGTGSGLGSFLLERMNDRFPKKLIQTYSVFPDTQAADVVVNPYNSLLAMRRLTQNADSVVVLDNAALSRIVADRLHVQEPSFQQTNRLVSTVMSASTTTLRYPR"
  },
  {
    "reference": "MILKILIMIPDGIFWNNKAEEIILPTNTGQIGILKNHAPLITALDIGVILIRTDKKWVPFIIMGGFALIKQNKITILVNGAESAGTLKLVQSEAAFQEATNKLENAKSKKQYVDALFLFKCAKARYQAAKQLVS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein localizes to the proton-transporting ATP synthase complex, catalytic core F(1), plastid membrane. The designed protein sequence is ",
    "response#1": "GIKAVDSMIPIGRGQRELILGDRQTGKTAIAVDAIINQRGGDVICIYVAVGQKQSTVANVVQKLTETGAMDYTIVVSATASDPAPLLFIAPFAGAAMGEEFMWQGRHVLIVYDDLSKQAAAYRELSLLLRRPPGREAYPGDVFNLHSRLLERACKLDDQFGGGSMTALPIIETQAGDVSAYIPTNVISITDGQIYLEPDLFYAGIRPAINVGISVH",
    "response#2": "GIKAVDSMIPIGRGQRELILGDRQTGKTAIAVDAIINQRGGDVICIYVAVGQKQSTVANVVQKLTETGAMDYTIVVSATASDPAPLLFIAPFAGAAMGEEFMWQGRHVLIVYDDLSKQAAAYRELSLLLRRPPGREAYPGDVFNLHSRLLERACKLDDQFGGGSMTALPIIETQAGDVSAYIPTNVISITDGQIYLEPDLFYAGIRPAINVGISVH",
    "response#3": "GIKAVDSMIPIGRGQRELILGDRQTGKTAIAVDAIINQRGGDVICIYVAVGQKQSTVANVVQKLTETGAMDYTIVVSATASDPAPLLFIAPFAGAAMGEEFMWQGRHVLIVYDDLSKQAAAYRELSLLLRRPPGREAYPGDVFNLHSRLLERACKLDDQFGGGSMTALPIIETQAGDVSAYIPTNVISITDGQIYLEPDLFYAGIRPAINVGISVT"
  },
  {
    "reference": "MMAANTVQTVATLGRLWPAGAPALPSEVAGQRVTDLAQDSRRVTPGAVFVALRGTQGHGLAHARAAAEQGAVAILWDEAEAAPALSIPVLHLPGLRVALPAICDALWGAWPADYPVVAVTGTDGKTSVSQLFAGALEQLGVPTGVVGTLGTGRFGALEDCGHTTPGVIDLHRHLAHLTADGCRALSIEASSHGLEQGRMAGLPVRVAVLTRLGSDHLDFHGSQQAYADAKARLFAWPGLETAVLNADDALGQRLIRETTAEHILTYSAVGASADLVAREVEAGSDGIRFTLEVAGEAWPVQAALFGLFQVGNLLATLAALRALGHSWPAAVDAVSRLRGVPGRMERFELPSGALLVVDYAHTEQALAAALDALRPHTAGRLIAVFGCGGDRDVTKRPAMGRAASARADQVIVTDDNPRSEDPAAIRAAVLAGCDRPEACRELGDREAAVRAAVAEAGPGDVILLAGKGHETTQQVGTQFYPFSDRELALALAARTNAGDSGGGIQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have magnesium ion binding, ATP binding within cytoplasm to regulate regulation of cell shape, cell cycle, cell division, peptidoglycan biosynthetic process, cell wall organization.2. The designed protein must have at least one Mur ligase N-terminal catalytic, Mur ligase central, Mur ligase C-terminal domain.3. The protein design must feature the Meso-diaminopimelate recognition motif motif.4. We require a protein that can specifically bind to the metabolites produced in the Cell wall biogenesis, peptidoglycan biosynthesis pathway.5. We are looking for a protein with broad substrate specificity for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.6. The protein should have a high affinity for ATP, meso-2,6-diaminoheptanedioate, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate.7. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "MNPVTGITHDSRAVRPGDIYAALPGARFHGADFAAQAASLGAVAALTDPAGAERVLAAGLPVLVVGDPRGRMGALAARIYGRPADRLALTGVTGTNGKTTITYMLKAIWEKDAEKTGLIGTVAYYVGEKKIPAVNTTPESLDLQRMFCEMLKQKVSSVAMEVSSHSLVLNRVKMVDFDVAVFTNLNPEHLDFHKDMRSYKEAKGILFQNLKEDSYAVINLDDPEWEYFYNVSKGVRLTYSLKNKEADFYVKTCSQTGSGFKLELVTPVGDLDIDLKVLGDVNIYNALAAVASVTGITHDSRAVRPGDIYAALPGARFHGADFAAQAASLGAVAALTDPAGAERVLAAGLPVLVVGDPRGRMGALAARILGVPQERIAAGIAASAGVPGRLERVDEGQPYLAVVDYAHKTDAVESVLRSLRRVTEGRVHVVLGCGGDRDTTKRGPMGAAAARLADTAVLTSDNPRSEDPLAILAAMLAGAAEVPVHERGDVLVDADRAAAIAAVVARAEPGDTVLIAGKGHEQGQEFADGTVIPFDDRAVAAAALEGRSR",
    "response#2": "MKLRDLIAPVEVEGIEAGDVALTADSRAVKMGSVFVAYPGTTRDGRAFITEAIAKGAAAVIWERCGFDWDERWEVPNLGVANLRHRISEIASRVYGRPAESLQLVGVTGTNGKTTTTYLIERILSDAGQSAGVIGTIETRYAGNTLPMSGTTPGALELQQYLAAMAEAGVDSCAMEVSSHALDQGRTWGCRFRIGVFTNLTQDHLDYHGTMEKYAAAKSLLFSRLGNTYSEAEAERSYAVLNADDSASDLFANAAAVEILTYGIDREADVRASDIRITSRGTTFRLETFRGGADISIRMVGKFNVYNALAATSAGLALGLELGRIRAGLASSPQVPGRMELVGKTKTGASVIVDYAHKPLALETVLKSLRAHVQGQIIVIFGCGGNRDQAKRKMMGEIAKDYADSIIVTDDNPRFEDPQAIRKQILEGVPEAYEIASREEAIAFALHKAEAGDICLIAGKGHEDYQEVRGVRHPFDDREVAREALAARRGKR",
    "response#3": "MIVTGITHDSRAVRPGDIYAALPGARFHGADFAAQAASLGAVAALTDPAGAERVLAAGLPVLVVGDPRGRMGALAARIYGEPSRQLRMIGVTGTNGKTTTTYLIERILSDAGKRVGLIGTIETIIGDEVIPAKNTTPESYVVQEYFRKMVDAGCECVVMEVSSQGLMLHRTAGFVFDYGIFTNLEPDHIGPNEHASFEEYLACKGRLFRQCRLGIANVEDSHAEKVLEGHTCKVETFGFSEAADLRAENVRMVPGGVAFTVNYEGQMDFDVEIDIPGKFSVYNSLCAIAICRHFGITEENIRQALRQVKVKGRMELVGKTKTGASVIVDYAHKPLALETVLKSLRAHVQGQIIVIFGCGGNRDQAKRKMMGEIAKDYADSIIVTDDNPRFEDPQAIRKQILEGVPEAYEIASREEAIAFALHKAEAGDICLIAGKGHEDYQEIAGVKYHMDEREVVREILEERKQ"
  },
  {
    "reference": "MSNRKIFVQDVTLRDGMHAVRHRISPTDVKRIVTALDAAGVDAIEVAHGDGLAGGSLNYGPGSNTDWEWIEAAAEVLEHARLTTLLLPGVGTISELKHAYDLGVRSVRVATHCTEADVSAQHIETAREIGMDVSGFLMMSHMAPAEELAKQAKLMESYGAHCVYVTDSGGRLVMNDVADRVRAYRDILDEGTEIGIHAHENLSLSVANSVVAVQNGVTRVDASLAGHGAGAGNCPLEAFIAVANIEEWEHGCDLFALQDAADDIVRPLQDRPVRVDRETLTLGYAGVYSSFLRHAEAASERYGIDVRTLLLEAGRRRLVGGQEDMIVDIALTLAAQATKEAV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein have Pyruvate carboxyltransferase domains with specific sequence motifs that are crucial for its activity.2. The protein must have high specificity towards manganese ion binding, 4-hydroxy-2-oxovalerate aldolase activity.3. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.4. The designed protein should have a binding site that is specific to the target substrate, Mn(2+) ligand. The designed protein sequence is ",
    "response#1": "LVTPEDLPFIIADVSGRTGEKVLTIKSCNIHSGIGIRPHAQIELEYQGKIHKEISEGDGGYDAFMNALTKITNRLGISIPKLIDYEVRIPPGGKTDALVETRITWNKSLDLEEDQTFKTMGVHPDQTVAAVHATEKMLNQIR",
    "response#2": "ITVHDMTLRDGMHPKRHLMTLEQMKTIAQGLDQAGIPLIEVTHGDGLGGSSVNYGFPAHTDEEYLGAVIPLMKQAKVSALLLPGIGTVDHLKMAHELGVNTIRVATHCTEADVSEQHISYARKLGMDTVGFLMMAHMNSPEGLVQQAKLMESYGANCIYVTDSAGHLLPDTVKARISAVRDALKPETELGFHGHHNLAMGVANSIAAIEAGATRIDAAAAGLGAGAGNTPMEVLIAVCDLMGIETGVDVFKIQDVAEDLVVPIH",
    "response#3": "VTPEDLPFIIADVSGRTGEKVLTIKSCNIHSGIGIRPHAQIELEYQGKIHKEISEGDGGYDAFMNALTKITNRLGISIPKLIDYEVRIPPGGKTDALVETRITWNKSLDLEEDQTFKTMGVHPDQTVAAVHATEKMLNQIN"
  },
  {
    "reference": "MKELVIATRNRGKMKEIEAFLAGLVERVTCAADFEAFPETVEDGATFEANALKKAREAMKFTGLPALADDSGLVVDALDGRPGVYSARFAGEGADDAANNRTLLHELAGVSPERRQAAFVCALAFISPEGEEKVFYGRVGGCILAEARGEGGFGYDPLFLVDGFGRTMAELSVDEKNRISHRGQALRQFREYLE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.2. The designed protein must be able to perform metal ion binding, dITP diphosphatase activity, ribonucleoside triphosphate phosphatase activity, nucleotide binding under specific conditions.3. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP The designed protein sequence is ",
    "response#1": "MNEVVFVTGNANKLREVKAIL",
    "response#2": "MKIVLATHNEGKMKEIREILGNLEIELLSLHDFPNIPEVVEDGKSFEENALKKAEYATKITGLPTLADDTGLEVEALNGAPGVYSARYAGEHGNDAANNRKLLEALKDVPDEKRGAHFKTVIALVLPNGKEYFAEGRCPGEILEKERGENGFGYDPLFIPDGYDQTFAEMSLEEKSKISHRGKALQKLAEILKETF",
    "response#3": "MPELTLFDLDGTLLPGDSLTPLMEEFLAEKGHTREFTERLYRSEG"
  },
  {
    "reference": "MSNKNKKSGGAGNGAAQKQTRQQSHDSQDYSSFKIVLFYCMLIVFLPVVTFFLLKGFVLDRFFSLSEVKVNIASAVGAVVSLHIALGLYIYRAYFGATGSKAVKED",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Required for the assembly of the V0 complex of the vacuolar ATPase (V-ATPase) in the endoplasmic reticulum.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. The protein should be designed to localize specifically to endoplasmic reticulum-Golgi intermediate compartment membrane, endoplasmic reticulum membrane in order to perform its role in vacuolar proton-transporting V-type ATPase complex assembly more effectively.4. The Lumenal topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "VPSLSRVASWEFNPLNVKVGSHITGGDLYGLVHENTLVKHKMIVNPRAKGTVRYIAPSGNYKVDDVVLETEFDGEITKHTMLQVWPVA",
    "response#2": "GQRVLDSLFPCVQGGTTAIPGAFGCGKTVISQALSKYSNSDVIIYVGCGERGNEMSEVLRDFPELSVEIDGVTESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYNVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSINWLISYT",
    "response#3": "VPSLSRVASWEFNPLNVKVGSHITGGDLYGLVHENTLVKHKMIVNPRAKGTVRYIAPSGNYKVDDVVLETEFDGEITKHTMLQVWPVQ"
  },
  {
    "reference": "MKFVAKYFPEIIMKSRPVRKRFVKQLQMNIRNLVARNGFKADVQGQWDNLYVGINSDDPAIRRAMINILTHTPGIAFSYEIEEHDFDTMDDAYQIIKAVNGPSLAGKTFCVRVKRRGTHDYNSNDVERYIGGGLLKETDAAGVKLKNPDVVVQVEIKDQSLFAIKHRFEGLGGYPLGSQGDTLSLMSGGFDSSVASYMMMKRGIKTHFCFFNLGGSAHEVGVKQVAYYLWDKYGGSHKARFISVPFDGVVKEILENVEDGHMGVVLKRMMYRAAELVADSVNLETFVTGESIAQVSSQTLTNLAVIDKVTDKLVLRPLVVMDKPEIISLARKIGTETFAATMPEYCGVISVKPTTEAKMARVLAEETKFDLAVLEKAVEDASHQFIGNLVKEENDSLEVACITLPSPSDIIVDIRHPNEVEDAPLILNHNDIINMPFYELETRKGEFKEDQSYLFYCQKGTMSKIHAHHLNKQGMDNIKVYLEEKPKMLDKAAEKAKKRAKFEAELKEALDEELKG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. We require a protein that can specifically bind to the metabolites produced in the thiamine diphosphate biosynthesis, Cofactor biosynthesis pathway.2. The protein contains novel Rhodanese, THUMP domains that confer a unique function or activity.3. The designed protein should be involved in thiamine diphosphate biosynthetic process, thiamine biosynthetic process by localizing to cytoplasm and exhibiting tRNA U4 sulfurtransferase, tRNA binding.4. For general function, the protein need meet that Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor; Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate; The sulfur is donated as persulfide by IscS.5. The protein should have a high affinity for ATP.6. The designed protein must have a Cysteine persulfide intermediate active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "LKLFPEITIKSRSVRRHMTRCLAANVRNTLKPLDRRIAVRDGWDLLRVSLPVSAGAALVDEVEARLTRMPGVQEVQEVREVDFSDLDEITAQVVPRWQAHVTGRRFRVSAKRRGRHDFSSEDLERHLGAALLAAAPDARVDLTHPEVKVTVEVVDQRLRLIDRRLPGLGGYPLGLQGQAMALISGGYDSPVAAWRMIRRGIKTHFLFFNLGGPEHERGVREVTHHLWQRYSASHRVNFISVPFEPVVGEILRRVPDGMMGVVLKRMMVRAASRIARRHRVPALVTGDAIAQVSSQTLTNLALIDDASELPLLRPLLTDDKQTIITQAREIDTARFAESMPEYCGVISRRPHTRARRDQIAAAEAGFDIVRLHAEITVKSKSLRKRHGSVLSSNIRNLIKQHTDQVAVRWLWDKIEVEIPADEQLAGKVRDVLVCIPGISHIEEVIETDFTDFEGALQFVIDNMGPALTHKSFAVRVKRRGKHEFSSQDLAVYIGGGIRARVEGATVKLVQPQETVQLWLENDTLSLLVRRLPGLGGYPLPTQETALSLISGGFDSAVASYQFIRRGARTHFCFFNLGGAAAHEAAVREVAYYVWKKYSSTHPVKFVSVDFSGVVEAILNQNEPGVMGVLLKRSMLRAAALVADRVNAQALVTGEALGQVSSQTLSNLQIIDEVTHKLVLRPLITMDKQDIVNIARDIGVEEMAASIPEYCGVISNKPTVKAKQQVVLAAEAGITDELKLFPEITIKSRSVRRHMTRCLAANVRNTLKPLDRRIAVRDGWDLLRVSLPVSAGAALVDEVEARLTRMPGVQEVQEVREVDFSDLDEITAQVVPRWQAHVTGRRFRVSAKRRGRHDFSSEDLERHLGAALLAAAPDARVDLTHPEVKVTVEVVDQRLRLIDRRLPGLGGYPLGLQGQAMALISGGYDSPVAAWRMIRRGIKTHFLFFNLGGPEHERGVREVTHHLWQRYSASHRVNFISVPFEPVVGEILRRVPDGMMGVVLKRMMVRAASRIARRHRVPALVTGDAIAQVSSQTLTNLALIDDASELPLLRPLLTDDKQTIITQAREIDTARFAESMPEYCGVISRRPHTRARRDQIAAAEAGFD",
    "response#2": "LKLFPEITIKSRSVRKEMVRCLRANVRNTLSRYDAGVRVTGNWDALEVQPSADVTEQDLAVIEAVMTRIPGIHEVLTIEDREFHSFAETAEQLLPLWGPYITHKRFCVRVKRRGEHEFRSDDLERHLGAALLAAAPGARVDLERPDIVVSLELKDKRLLLITQRRPGLGGYPLGTQGAALALISGGFDSPVAAYRMIRRGIKTHFLFFNLGGPAHEAGVREVTHHLWQRYSTSHHVNFISVPFEGVVNEILTKIPDGLMGVVLKRMMMRAASKIAAKNRIPVLVTGDAIAQVSSQSLTNLCLIDALVERPILRPLVANDKQDIITEARHIGTAEFAERMPEYCGVTSRRPNTRARRDRVEEAEAEFDALPGQIILDIRHPDAAEDEPLALPGIEVQTLPFYAINSRFKELDANRQYLLYCDKGVMSRLHAHHLLSEGHANVRVEDNAVVLDIRSAEEEEASPLELDSVEVKHLPFFKLATQFGDLDQSKTYLLYCDRGVMSRLQALYLQEQGFSNVKVRVEKHSIILDIRSPDEKDNTPLKIDGAKVQHLPFFRLSTQFGSLDQNKTYLLYCAHGIMSRIQALHLQEDGFDNVKIR",
    "response#3": "MLKLFPEITIKSRSVRRHMTRCLAANVRNTLKPLDRRIAVRDGWDLLRVSLPVSAGAALVDEVEARLTRMPGVQEVQEVREVDFSDLDEITAQVVPRWQAHVTGRRFRVSAKRRGRHDFSSEDLERHLGAALLAAAPDARVDLTHPEVKVTVEVVDQRLRLIDRRLPGLGGYPLGLQGQAMALISGGYDSPVAAWRMIRRGIKTHFLFFNLGGPEHERGVREVTHHLWQRYSASHRVNFISVPFEPVVGEILRRVPDGMMGVVLKRMMVRAASRIARRHRVPALVTGDAIAQVSSQTLTNLALIDDASELPLLRPLLTDDKQTIITQAREIDTARFAESMPEYCGVISRRPHTRARRDQIAAAEAGFDIIKLHPEITIKSKSVRQRMSKILQGNIRNVLRPLDEHLKIRADWDKLVVRTHIQTEEHRLAMVAALACIPGIQSFLEVTEHRVETMDEILELTQAMFGDRLAGKTFCVRAKRRGQQAFSSLDVERYVGGGLNQRCVTGGVRLKHPDVQINLELDGDRLFLVSEQHAGLGGFPIATQESVLSLMSGGYDSGVASYQFIKRGSRVHYCFFNLGGQEHEAGVREVVYYLWKKYGASHRVKFVSVPFEGVVAEILAKVDKGHMGVVLKRMMMRAGAAVAGKLAINALVTGESMGQVSSQTVTNLNVIDRVTDMLILRPLIAADKQDIIDQARQIGTAPFAEKMPEYCGVISQKPTIKARLGKVEAEEAHFDLAMVAALACIPGIQSFLEVTEHRVETMDEILELTQAMFGDRLAGKTFCVRAKRRGQQAFSSLDVERYVGGGLNQRCVTGGVRLKHPDVQINLELDGDRLFLVSE"
  },
  {
    "reference": "MHHKVNVKTQREVHFPMDLLQACGASAPRPVARVSRATDLDRRYRCVLSLPEERARSVGCKWSSTRAALRRGLEELGSREFRRRLRLADDCWRAICAAVCTGRKFPSFSVTDRPARARLAKVYRMGRRLLVGVVCRGESVVSDLKQECADLRRVIFEGSTRIPSSSLWGLVGVLGWTSPERAMQLTFIGRALPYGSPDVERRALASHAATLSIPAECHPNYLVAAEQFAKSWADDNLPRKFRIYPIAVQESSCMEYSRAQGGLLQSFRKGFVGYDPAAPSADPDDLELAKERGFSRIRASWYSTFRYRGELKSTNQSLEARVAVVPERGFKARIVTTHSASRVTFGHQFRRYLLQGIRRHPALVDVIGGDHRRAVETMDGDFGLLRPDGRLLSADLTSASDRIPHDLVKAILRGIFSDPDRRPPGTSLADVFDLVLGPYHLHYPDGSEVTVRQGILMGLPTTWPLLCLIHLFWVELSDWAPARPNHSRGFVLGESFRICGDDLIAWWRPERIALYNQIAVDCGAQFSAGKHLESKTWGIFTEKVFTVKPVKMKVRVRSEPSLKGYVFSRSSAFSCRMGGKGITGIRAARLYTIGAMPRWSRRIRDVYPGSLEHRTASQRYGEPVTVYRFGRWSSAIPLRWAVRAPTRTVGNPVQSLPDWFTVGPAASSVAADSNAFGAVSRVLRRMFPGLPRKLASAGIPPYLPRVFGGGGLVKSTGLTTKIGAVASRRWMSRIGHDLYRSRERKSTLGRVWTLSTSPAYAASLHEVE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must have high specificity towards RNA-dependent RNA polymerase activity.2. The designed protein's functionality will benefit from Pro residues compositional bias in its structure.3. The protein should be designed to catalyze the following reaction a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +   RNA(n+1). The designed protein sequence is ",
    "response#1": "ASMLPLDKCFKASFCGDDSLIYLPKGLEYPDIQATANLVWNFEAKLFRKKYGYFCGKYIIHHANGCIVYPDPLKLISKLGNKSLVGYEHVEEFRISLLDVAHSLFNGAYFHLLDDAIHELFPNAGGCSFVINCLCKYLSDKRLFRR",
    "response#2": "QSPGIYYQSELDHKGISVYTGTIISDWGGRLELEIDRKARIWARVSRKQKISILVLSSAMGSNLNEILENVCYPEIFLSFLNDKEEKKIGSKESAILEFYRQFACVGGDPVFPESLCRELQKKFFQQRCELGEIGRRNMNRRLNLDIPQNNTFLLPRDILTAADHLIGMKFRMGTLDDMNHLKNKRIG",
    "response#3": "ASMLPLDKCFKASFCGDDSLIYLPKGLEYPDIQATANLVWNFEAKLFRKKYGYFCGKYIIHHANGCIVYPDPLKLISKLGNKSLVGYEHVEEFRISLLDVAHSLFNGAYFHLLDDAIHELFPNAGGCSFVINCLCKYLSDKRLFRR"
  },
  {
    "reference": "MSHINVEKKLNFVNKLNNVNNRVLKIENLALAYDGKRILDNINMFMERGDIITILGPNGGGKTSLVKAIAGINKNYTGNIVLAENTKISYMPQNFSISNLMPITVEYFLLNSSFKRLKKNQSIITETIELAGIGNILKNQVLEISAGQTQLLLLARCLIAEPDLIILDEPVSAMDINARAKFYDIINKIAKKRLVSILMTSHDLNSALPSSDYIICINNTIYCQGKPDEIMKNRTLNEIFSSYAAK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit ATP binding, ABC-type zinc transporter activity.2. The designed protein must have at least one ABC transporter domain.3. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.4. The protein should have a fast turnover rate for ATP(in) + H2O(in) + Zn(2+)(out) = ADP(in) + H(+)(in) +   phosphate(in) + Zn(2+)(in).5. For general function, the protein need meet that Responsible for energy coupling to the transport system.; Part of the ABC transporter complex ZnuABC involved in zinc import The designed protein sequence is ",
    "response#1": "MLVKGSVFLEQLGSVQMIAFDKTGTVTKGQPAVETIRIAEGFSEAEVLEAVYAIETQSSHPLAQAITAYAESRGVNQSGYISIEETSGFGVMAEVSGAKWKVGKAGFIGEEMAAQFMKQTASDVIQSGHTIVFVKKDDQIAGCIALKDQIRPEAKEVMEELNRLGIKTAMLTGDHEDTAQAIAKEAGMTTVVAECLPDQKVNEIKRLKEEFGTIAMVGDGINDAPALKAADVGIAMGGGTDVALETADMVLMKNDLKKLVNMCRLSRGGLRRLGEGEFICVIGHSGCGKSTLLNMVSGFNQPSHGSVRLKGKEIDRPGPDRMVVFQNYALLPWMTAYENVYLAVDCVNPQMREGEKREIVREHLAMVGLTEAAEKKITQISGGMKQRVAIARALSIRPEVLILDEPFGALDAITKEELQEELLKIWNDHRCTVLMITHDIDEALFLADRLVMMTNGVDVPIGVSKELITS",
    "response#2": "IGCASLTIQLGKRTILKDISMGVEPGEIVTIIGPNGSGKTTLLRAMIGAVAPRSGRLIRAPGLTLGYAPQTLHIDETLPMTVQRFMSLPKLGTASDIDAALSQAGVPGLEKQQIMSLSGGQLQRVLLARALLGRPRLLLLDEATQGLDPRGVADFYRQITSVRDELGCAIIMVSHELHLVMRKTDRVICLNGHICCEGEPDIVSRSPEYLALFGIK",
    "response#3": "IGCASLTIQLGKRTILKDISMGVEPGEIVTIIGPNGSGKTTLLRAMIGAVAPRSGRLIRAPGLTLGYAPQTLHIDETLPMTVQRFMSLPKLGTASDIDAALSQAGVPGLEKQQIMSLSGGQLQRVLLARALLGRPRLLLLDEATQGLDPRGVADFYRQITSVRDELGCAIIMVSHELHLVMRKTDRVICLNGHICCEGEPDIVSRSPEYLALFGIG"
  },
  {
    "reference": "MSDEHPPQPKAFPVSWDQFHRDARALAWRLSGQGEWEAIVCITRGGLVPAAIVARELGIRVIETVGVASYHEYKEQGSLVVLKGVDPKITALGGGEGKGVLIIDDLVDTGSTARIVREMLPKAHFATVYAKPKGRPLVDTFITEVSQDTWIYFPWDMGLTFQPPISKGTLG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that To a lesser extent, also acts on hypoxanthine.; Purine salvage pathway enzyme that catalyzes the transfer of the ribosyl-5-phosphate group from 5-phospho-alpha-D-ribose 1- diphosphate (PRPP) to the N9 position of the 6-oxopurines guanine and xanthine to form the corresponding ribonucleotides GMP (guanosine 5'- monophosphate) and XMP (xanthosine 5'-monophosphate), with the release of PPi2. A protein that can modulate the overall flux of metabolites through the XMP from xanthine: step 1/1, XMP biosynthesis via salvage pathway pathway, enhancing or suppressing its activity as needed.3. The ideal protein would be one that plays a critical role in XMP salvage, purine ribonucleoside salvage, GMP salvage through guanine phosphoribosyltransferase activity, magnesium ion binding within plasma membrane.4. The protein have enhanced diphosphate + XMP = 5-phospho-alpha-D-ribose 1-diphosphate +   xanthine.5. The designed protein must have at least one Phosphoribosyltransferase domain.6. The protein should have a high affinity for Mg(2+), guanine, 5-phospho-alpha-D-ribose 1-diphosphate, xanthine, GMP. The designed protein sequence is ",
    "response#1": "MISWDQIHRDSRALAWRLDKRGPGEGGAWKAVVAITRGGMAPAMILARELDIRIVDTISVKSYNHQAQMDAKVLKAPDAELMGDGAGILVVDDLVDSGKTLELVRRLYPKAHFATVYAKPKGRPMVDTFITEVSQDTWIFFPWDMALQYEPGDAA",
    "response#2": "MESWDQIHRDSRALAWRLDKRGPGEGGAWKAVVAITRGGMAPAMILARELDIRIVDTISVKSYNHQAQMDAKVLKAPDAELMGDGAGILVVDDLVDSGKTLELVRRLYPKAHFATVYAKPKGRPMVDTFITEVSQDTWIFFPWDMALQYVEPYRGRS",
    "response#3": "MKISWDQFHRDTRALTWRLHEAGPFEAIVCITRGGLVPAAIVARELNVRIIETVCIASYHDYKNQGDIKVMKGVGPEITSMRGQGKGVLIVDDLVDTGKTARVVREILPAAHFATVYAKPMGRPLVDTFITEVSQDTWIYFPWDTGLETHTDPRQSPKSWDQIHRDARALAWRLDRQGPGPDGSWRAVVAITRGGMAPAMIVARELDIRVVDTISVKSYDHQARSEAEVLKPPVAEMIGEGEGVLIIDDLVDTGKTLELVRRLYPKAHCATVYAKPKGRPMVDTYITEVSQDTWIFFPWDMALQYIDPME"
  },
  {
    "reference": "MRGRPGVYNILYAYKDAETGLAAGAFAIALVLSLLPEGLRSIEGARRLGMELPSDPQNISAMVETLRAILRRNSLGPTTAALMQAAERRGIPVMRLNDQSLIQLGLGSRQKRIRASITADTSQIAVDIAGDKDLTKRLLDEAGLPVPRGAVYRQADEALAETRRLGWPLVVKPLDGNHGRGVTTGVRDEGTLRAAFDHALRHGRRVIVEQQLPGHDHRILVIGGKVAAVAERLPAHVMGDGRRTIAELIDLVNQDPRRGMGHENMLTRIVPDAAMTALLARSGHDLGTIPAPGQIVQLRDTANLSTGGTAVDRTDDIHPCNRLIAEQAAATIGLDVCGIDFLSPDISRPVSETGGGIVEVNAAPGFRMHLDPSSGQPRDVAAPVIDALFPRGRSSRIPIFAITGTNGKSTTVRMVERILTHHGLNVGMTTTSGIYVGGQLLKAVDASGPRSARSVLRNPTVDAAVLETARGGILREGLGFDGADVGSVLNVTPDHLGLKGIDTLEDLANVKAVVVESVSRRGYSVLNADDPMCVRIARHARGTIVWFSLNGGSRIPELLRRHIESGGTALVREDGADGGMLVIHRSGERKELMPAAAIPATLDGIADFNIANALAAAAICFVHGIPADTIRQSLVQFTNSFADSPGRLNIRDAHDRRFIVDYAHNPAGLAALGQVVDALRSRHRRVIGMVSIPGDRRDSDIIEMGSIAAGIFDEIIFREAPDGRGRPPGETNGLMSEGALSAGTPADRIHRIVDELEAVQATLDRGEA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The presence of ATP-grasp domains is critical for the interaction of the designed protein with its intended targets.2. The designed protein must possess tetrahydrofolylpolyglutamate synthase activity to support cell cycle under certain conditions.3. The desired protein should be able to perform [L-4-(L-arginin-2-N-yl)aspartate](n)-L-aspartate + ATP + L-   arginine = [L-4-(L-arginin-2-N-yl)aspartate](n+1) + ADP + H(+) +   phosphate The designed protein sequence is ",
    "response#1": "REAMERIGLRVPESRTVRSVPEAEELAERIGFPLIIRPSFTLGGTGGSVAEDLDALRASVRDGLDASPVRSVLVERSVAGWKEFELEVMRDLADNVVIVCSIENVDPMGVHTGDSITVAPAQTLSDRQYQSLRTAAIRIIREIGVSTGGSNIQFAVNPEGDDFYVIEMNPRVSRSSALASKATGFPIAKIAARLALSGDVETNLGKLTKREGQPLFTACTPKGIMCILKHYGINVQGKHAVVIGRSNIVGRPMSILLEKANATVTLCHSKTESIADIVRTADIVVAAIGIPHFVKADWLKKGVVAIDVGINSIPDATKKSGYRLTGDIDFENAKEVASAITPVPGSVGPMTVAMLLQNVVESAVEAAD",
    "response#2": "REAMERIGLRVPESRTVRSVPEAEELAERIGFPLIIRPSFTLGGTGGSVAEDLDALRASVRDGLDASPVRSVLVERSVAGWKEFELEVMRDLADNVVIVCSIENVDPMGVHTGDSITVAPAQTLSDRQYQSLRTAAIRIIREIGVSTGGSNIQFAVNPEGDDFYVIEMNPRVSRSSALASKATGFPIAKIAARLAVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPESAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVDADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKVPMHPEKDVDGFCETNLGKLTKREGQPLFTACTPKGIMCILKHYGINVQGKHAVVIGRSNIVGRPMSILLEKANATVTLCHSKTESIADIVRTADIVVAAIGIPHFVKADWLKKGVVAIDVGINSIPDATKKSGYRLTGDIDFENAKEVASAITPVPGSVGPMTVAMLLQNVVESQ",
    "response#3": "ITGTNGKTTVTSLVGQLVQRAGKTVAVAGNIGPTLLDTLSAHLDADTLPQVWVLELSSFQLADAQDFEPTAAVVLNVTQDHLDWHGDMDAYVAAKARIFGRSTFMLLNRDDPRVMEMLPTPVKVRLRPPQVRPHSTFGAGEPQRPGDYGIETVNGMAWLVRAAQADETIKRRKGEEVELHIQRLMPLDALRIRGRHNATNALAALGLAISMGADPVKVFSTLPQLRGVRGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLHDKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADAKAEDTVLVCGSGDVWTVGQTIAEQYASG"
  },
  {
    "reference": "MELPPAVSLMGLLLLLLLGQGPSQIGSSSIENTLAWQPQEVLRTLKNFAFPCKPRKLGLSTPSKSVHSLTPSDIKLVAAIGNLGTPPAPGMVTMEKTQSIKNEPQHVCMEAMTVLSDIIRHFNPSVLMPTCSPGKSDAAHTTVEDLWTQAKELVRHLKEKPQLDFENDWKLITVFFSNTSQCHLCPSAQQKRHLMRSMEKLCEILDYLHQEVPRAFVNLVDLSEVLAMSHQHQETGFSPEPELCDCSEETTKLSKAIMQWSYQETWEGLLASSKFNDHESFTVVFQPFFYEVEPSTEMPEAQDPSTLALRIWNNMMKPVGHKDELIDAVERKTMKCPSQESPYLFTYRNSNYQAKQLKPTAKLQVREGTEFSCSDKDPSDTVPTTVHRLRPADIKVIGAMGDSLTAGNGAGSRPGNVLDVLTQYRGLSWSVGGDENIRTVTTLANILREFNPSLKGFSVGTGRETSPQASFNQAVAGAKAEDLVTQAQRLVSLMQADTTINFQEDWKIITVFIGGNDLCASCNDLVHYSPQNFTDNIKKALDILHAQVPRAFVNMVEVIEIIGLRELYKETEVSCPRLILRNLCPCVLKFEDNSAEFAALVERNRQYQIKTEQLIKSGRYDTRDDFTVVLQPFFENVAVPRTPEGLPDNSFFAPDCFHFSGKTHARSAIALWRNMLEPVGHKTRQYDFENKIPILCPNQTLSFLSTYKNSNQGYGTWTLCKDRAPSASPPTSVHALRPADIQVVAALGDSLTAGNGISSQEGDLSDVS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have phospholipase A2 activity, triglyceride lipase activity and be active in apical plasma membrane.2. We are looking for a protein with broad substrate specificity for a triacylglycerol + H2O = a diacylglycerol + a fatty acid +   H(+).3. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "LADLAANGVGAKADNLLLVINEYRGLSWSIGGDENITSVTTLPNILREFSSNLTGFSEGVGSEDSPKAFLNQAVAGAKSGDMVRQVRVLVDKMKEDVRIDFHNDWKVITMFIGGNDLCDFCTDSVIFSPRNVASRIRQALDILHSEVPRAIVNLIELLDIVPLRDLHRDKSLGCPTWFVGLICPCVLKPKEGSSELHKVEDFNRAYQHAMRELLDSGRYDTRDNFTVVLQPFMRDILLPKLENGRPDRSFFSPDCFHLSQKAHTLMARALWNNMLEPVQ",
    "response#2": "SVHRLKLADIKVIGALGDSLTVSYGWWKEGQEKRRSWLIGDVGGDQNLSSVITLPNMLREFNPSLKGFSVGKGSESSSGAYLNQAVPGAQAEDLPVQARRLVDLMKNDVNINFQEDWKIITLFIGGNDLCDVCDDPVRFSPQNFVDNIGQALDILHAEVPRAFVNLVKVLEIINLRKLYQDKNVSCPRLILRNLCPCVLKYDDNATELASLVEVNKKYQVSWELLCGSG",
    "response#3": "SVHELRPADIKVVAAVGDSLTAGNGIASSPNNILDVLRQYRGLSWSIGGDENITTVTTLPNILKHFNQNLTGYSVGMGKQQTPQAFLNQAVAGAKSKDLPSQAQALVARMKNDSRINFESDWKVITVFIGGNDVCDHCYNSLLYSVENYVRNVRETLDYLHKEVPRALVNLIEPLHITPLREMHTDASVKCPTWLVNILCPCVILPKANSKALQMVEDLNRGYQRLLHELVESSWYDTRSDFTVVIQPFFREIVVPRLPDGRPDRSFFSADCFHLSQRAQTQMARSLWNNMLEPLGWVTQYTHLP"
  },
  {
    "reference": "MSLSVVILAAGKGTRMRSSLPKVLHLVADKPMVAHVIDSARHLGASNIYVVYGFGGEVLKTALNSDGCGADLTFVEQTKQLGTGHAVDQASPFLKDDEDVLVLYGDVPLTKVATLENLLKAKPENGLALLTVHLANPTGYGRIVRADNQVVGIVEQKDANAEQLTIKEANTGILLANGGDLKRWLSGLSNKNAQGEYYLTDIIAAAHQEGKSIATAHPETEIEVEGANNRVQLASLERAFQVRLAENLMINGASLRDPARIDIRGSLAIGSEVSIDINCIFTGKVTLGNSVQIGANCILKNCVIGDNVEIKPNSIIEDAHIAESCSVGPFARIRPGTIMHKGSHVGNFVEMKKTTLGEGSKAGHLTYLGDTLIGKNVNIGAGTITCNYDGVNKSQTIINDNAFIGSNSSLVAPVIIGEAATVGAGSVIAKEVEKDDLAVARGKQRNIKNWQRPVKK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein contains novel MobA-like NTP transferase domains that confer a unique function or activity.2. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc)3. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.4. A protein with glucosamine-1-phosphate N-acetyltransferase activity functions is required to enable cell morphogenesis, peptidoglycan biosynthetic process, regulation of cell shape.5. The protein with the reaction acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate, the Proton acceptor must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MALAIVLAAGKGTRMKSDLHKVLHPLAGRPMLLHLLDNVAALAPARTVVVVGAGRAQVEAAVAPLGAEVVHQTEQLGTAHAVRQAETALGGFAGDVLVLYGDVPLIGTATMREMLDRLHAADRPAAVLTALVDDPTGYGRAIRDDAGVVVAIVEQKDATAEQLAITEINSGVYAFDAAVLRDGLSRITTDNAQGELYLTDVLGLARNSGRRVAGVVAEDPWLVEGVNDRVQLAVLAAEMNRRIVRRWQLEGVTVMDPTRLDIRGTVQTGRDVSIDVNVILEGEITLGDRVKIGPHTVIRHAKIANDVEILSHCVIENVVIGAGCRIGPFARLRPDTVLAEQVHVGNFVELKKSTVANGSKINHLSYVGDSKVGSGVNIGAGTITCNYDGANKHKTIIEDRAFIGSNTQLVAPVKVGAGATIGAGSTITINAPPDALTYTRSPQRNIEGWVVRKRAGTAAEEAAAAALAEHDTGDNE",
    "response#2": "MMAVILAAGQGTRMKSKLYKVLHPVCGKPMVSHVVDNVRGVGADRVVTIVGFGAEKVEEEVGGRCEFVLQEKQLGTGHAVLQAEQNLGGLDGTTIVVCGDTPLITPETMKALIDHHNQTKAKATILTVTSSDPEGYGRIVRNGPAEVLEIVEEKDATAAEKAIREINSGIMVLPTARLKEWLGRLSNNNAQGEYYLTDIIAMAVADGVPVNTVQPAHVWETLGVNNKLQLQQLERAHQLRQAQALMEQGVTIQDPANTYISADAEIGRDTVLMPGTVIEGRTVIGEDCLIGPGSQIRDSVIGDRTAIRQSVVMSSKIGEDTAVGPFAHIRPETDLGNSVKIGNFVEVKKSNLGEGSKVSHLSYIGDSDIGERVNIGCGTITVNYDGVNKFRTTVEDDAFVGCNSNLVAPVTVGKGAFVAAGSTVTKNVPENALAIARSRQVNITGWKRPVKVKK",
    "response#3": "LVLAAGHGSRMKSRVPKVLHHVAGQTMIDWVLDAVAPLTPNRPITVIGVGAESVQTHVGERSDFVLQAQQLGTGHAVQQAQDKLSASHGVTLIMSGDTPMFRAETLSEFIAAHRQSNNAVTVLTAVTQEPTGYGRIVRADDDTVLKIVEQKDASVTERRIQEINTGVYVFDNQLLFEALAQVKNNNAQGEYYLPDTLDILRQAGHQIGAEKVWQDGVRKAFMDNGVHMPAPETVFFSYDTEIAAGVTIEPNVVFAEGVTVETGAVIRAFSHLEGAKVGEGALIGPYARLRPGADIGRHVHIGNFVEVKNVTIGDGAKANHLSYLGDGSVGAGSNIGAGTIFCNYDGYFKYKTNIGERAFIGSNTSLVAPVTIGDGAMTGSGSVITADVAPGALAFERAQQVNNKAQRADLAEAEAAFQARMRAQMMADGVTLIAPETVFFAHDTKIERDVVIEPNVVFGPGVVIEEDVVIHAHSHVEGAHLKRGAHAGPFARLRPGAELGEGSKVGNFVEIKKSQLAEGAKVSHLTYIGDASIGANANIGAGTITCNYDGYDKHRTVIGENAFIGSNTCLVAPVTVGAGAFTATGTIVTEDVPADALALARTPQRSIAGWAARFEKLMKNGVIMHLPKTIFIDSRAKFEGECIIEPNVMIIGNSVIKNSVIKSGSVIEYSQVDSSDVGPMAHLRPNSKIKKTHIGNFVELKNANLDTVKAGHLSYLGDCDISSGTNIGCGTITCNYDGKKKYKTIIGKNVFIGSDTQLVAPVSIADDTLVAAGSTVTKDSNKGDLIITRNAQKQ"
  },
  {
    "reference": "MENRWQVMIVWQVDRMRIRTWKSLVKHHIHISKKAKGWIYKHHYESTNPRISSEVHIPLGDAKLVVTTYWGLHTGERDWHLGQGVSIEWRERKYSTQIDPGLADQLIHQYHFDCFSESAIRNAILGNRVRPRCEYQAGHNEVGSLQYLALTALITPKRIKPPLPSVKKLTEDRWNKPQKIKGHRGSRTVNGH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "ANVHIIMTSKRGKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTYY",
    "response#2": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC",
    "response#3": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC"
  },
  {
    "reference": "MTTTQFDNVALTTQANVYFDGKCVSHSFRLADGTQKSVGVVLPAELTFNTGAAEIMECVGGACEYRLKGSDQWLRSQAGERFEIPANSSFDIRVSEAYHYICHYA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be optimized for thymidine phosphorylase activity, uridine phosphorylase activity, guanosine phosphorylase activity, pyrimidine-nucleoside phosphorylase activity. The designed protein sequence is ",
    "response#1": "GNKGQ",
    "response#2": "MM",
    "response#3": "EVEPNG"
  },
  {
    "reference": "MDKLSSKIRAEFLGFFKNSGCAVVPSDSLIPAGDKTLLFTSAGMVQFKQHFLGQSKDSFTRATSCQKCFRTSDIDQVGTTARHLTFFEMLGNFSFGDYFKKEAAAWAWEFLTKNMSLPKDKLYITIYKDDDEVAGIWKNIAPANKIIKMDEKTNFWNMGETGPCGPCSEILIDLGQETGCGSPACCPECNCDRYLEIWNLVFTQFDKQPDGSLKNLPRKNIDTGMGLERLSATVNGRKNVFDTDLFMPVMENAAEILKIKNEGSNISKLRMIADHSRAITFLISDGILPSNEGRGYVLRRILRRALRQGKFYGYNKPYINELVSDVLKIMEGAYPELSSKLSNIRSIVKTEEEKFLETLESGSEILSSLINSYKSKDINIISGKDVFKLYDTYGFPHDLTKEMAFENGLEIDEDKFKFEQKKAQEKSRVAWCGSGERDITFYSILRKKTGDTVFTGYDNYASESRVLALIKDGSEISELKTGDNGEIILSHSSFYAQSGGQSDDKGKIANNSFESIVEYIFKPAGNLFVHKVKVLKGLIKINDAVSTIIDIERRKQIARHHTAAHLLHKALREAFGGHITQAGSLVARDYFRFDFTHFSALKKDDLIKIEKRVNSIIRLNSEVCIETMAIAKARNAGAMALFGEKYGDEVRTVLIKNESGDGNYSMELCGGTHVSRTGDIGIFKIISESSAAAGVRRIEAVAGIAAENYILDEEAVIIKTSKILNASKEELVNKAHKYTSDYKKLENEFKSLKSSLISGEIDSYAKEV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.; Catalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala)2. The designed protein should have a binding site that is specific to the target Zn(2+) ligand.3. A protein that localizes to cytoplasm and can perform tRNA binding, ATP binding, alanine-tRNA ligase activity, zinc ion binding with high specificity and efficiency.4. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.5. We are looking for a protein with broad substrate specificity for ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-   tRNA(Ala). The designed protein sequence is ",
    "response#1": "MLSNTIRSNFLKFYANRHHTILPSSPVFPHNDPSILFTNAGMNQFKDIFLNKEKVSYSRATTSQKCIRAGGKHNDLDNVGHTSRHLTFFEMLGNFSFGDYFKAEAIAFAWEVSLSVFNFNPEGIYATVHEKDDEAFALWEAYLPTDRIFRLTDKDNFWSMANTGPCGYCSELLFDRGPSFGNASSPLDDTDGERFLEYWNLVFMEFNRTSEGSLLALPNKHVDTGAGLERLVSLIAGTHTVFEADVLRELIAKTEQLSGKVYHPDDSGAAFRVIADHVRSLSFAIADGLLPGNTERGYVLRKILRRSVNYGRRLGFRNPFLAEIVPSLADAMGEAYPELKNSLSQIQKVLTLEEESFFKTLDRGGNLLQQVLKSSSSSSCISGEDAFKLKDTYGMPIDEISLLAKDYDYSVDMDTFHKLEQEAKERSRKNVVQSQGTSESIYNELHLTSEFIGYDHLSCDTFIEAIISKDHIVSSLQEKQEGAIVLKVSPFYAEKGGQVGDSGEIFCSEGTFIVTHTTSPKAGLIVHHGRISQGSLTVEAAVTAQVNRYRRKRIANNHTACHLLHKALEITLGDHIRQAGSYVDDTKIRLDFTHPQAISPEDLLCIETLVNESIRENEPVDIREALYSDVMNSSEIKQFFGDKYSDVVRVVSAGHSHELCGGTHAEATGDIGFFRITKEHAVAMGIRRIEAVTGEKAEATVHGMLEELKDLKGKIKNSSVADYVANIREELRQLRDTQKSLEKELSAAKASNLLSQAEEINGVKVVA",
    "response#2": "MNSSEIREKFLKFFESKGHTIVASSPLVPGNDPTLMFTNSGMVQFKDVFLGEDKRPYTRATSVQACLRAGGKHNDLENVGYTARHHTFFEMLGNWSFGDYFKRDALKWSWELLTQVYGLPAEKLLATVYIEDDEAFDIWTKEIGLPPERVIRIGDNKGGRYKSDNFWMMADTGPCGPCSEIFYDHGPHIAGGPPGSPDEDGDRFIEIWNNVFMQFDMAEDGSVKPLPAPCVDTGMGLERLAAILQHVHSNYEIDTFEALIKAAGRETNTKDLSNNSLKVIADHIRATAFLVADGVIPSNEGRGYVQRRIIRRAIRHGYKLGQKKPFFHKLVKDLVEQMGEAYPKLKADAQRITDVLKAEEERFFETLANGMEILDAALAGDAKVLPGEVAFKLHDTFGFPLDLSADVCRERGLSVDEAGFAAAMEKQKAAGRAAGKFKMERAVEYTGAGNTFTGYEHLEEQATVVGLYFEGTAVPSLKEGQAGIVVLDTTPFYSESGGQVGDQGFISAEGVQFGVEDTQKIKADVYGHHGVQTQGTLTVGDKVKAAVDGARRAATMRNHSVTHIMHKALREVLGDHVQQKGSLVDPDKTRFDFAHNAPVTREQILEIEKRVNAEILLNSDTQARVMDIESAQKTGALMLFGEKYGETVRVLDIGSSRELCGGTHVSRTGDIGLFKVMSESGVAAGVRRIEAITGAKALGYVQASLRDIRATFLDYFAKHEHEVVPSAPLVPQDDPTLLFVNAGMVPFKNSFTGQEKRASLRATSSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKEEAISHAWNLVTGEFGLPAEKLLVTVYAEDEQARALWRKIAGLTDDRIIGISTSDNFWSMGDTGPCGPCSEIFFDHGPSIAGGPPGSPDEDGDRFIEIWNLVFMQYEQLGPDERINLPKPSIDTGMGLERISAILQGKHNNYETDLFRNLIAAGESVLGVKAEGDALASHRVIADHLRSTCFLMADGVTPSNEGRGYVLRRIMRRAMRHAHLLGAGEPVMWKLVDALKSEMGDAYPELERAQALLEETLQVEEERFQRTLGRGLSLLEEATADMGEGDALAGATAFKLYDTYGFPLDLTQDALRAKGMTVDEAGFNSAMDKQRADARASKFSSGDAAPDAVWFAVRDKVGATAFTGYAGTGGKGRLTAIVSGGQEAKALGSGQRAELVFDETPFYGESGGQCGDTGEIRFVDGAVFTVEDTQKRGGDMHAHIGVLTKGEITLGDVAALDADAPRRAAIRANHSATHLAHAALRDVLGAHVTQKGSHVGPDRLRFDFSHNKSVSADQIAAIEAQVNAVIRQNVPVSTREMTPDAAIEAGALALFGEKYGDTVRVLAMGQGLADHATPYSVELCGGTHVERTGDIALFKIIAETAVSSGIRRIEAMTGEGARLYMD",
    "response#3": "MLSNTIRSNFLKFYANRHHTILPSSPVFPHNDPSILFTNAGMNQFKDIFLNKEKVSYSRATTSQKCIRAGGKHNDLDNVGHTSRHLTFFEMLGNFSFGDYFKAEAIAFAWEVSLSVFNFNPEGIYATVHEKDDEAFALWEAYLPTDRIFRLTDKDNFWSMANTGPCGYCSELLFDRGPSFGNASSPLDDTDGERFLEYWNLVFMEFNRTSEGSLLALPNKHVDTGAGLERLVSLIAGTHTVFEADVLRELIAKTEQLSGKVYHPDDSGAAFRVIADHVRSLSFAIADGLLPGNTERGYVLRKILRRSVNYGRRLGFRNPFLAEIVPSLADAMGEAYPELKNSLSQIQKVLTLEEESFFKTLDRGGNLLQQVLKSSSSSSCISGEDAFKLKDTYGMPIDEISLLAKDYDYSVDMDTFHKLEQEAKERSRKNVVQSQGTSESIYNELHLTSEFIGYDHLSCDTFIEAIISKDHIVSSLQEKQEGAIVLKVSPFYAEKGGQVGDSGEIFCSEGTFIVTHTTSPKAGLIVHHGRISQGSLTVEAAVTAQVNRYRRKRIANNHTACHLLHKALEITLGDHIRQAGSYVDDTKIRLDFTHPQAISPEDLLCIETLVNESIRENEPVDIREALYSDVMNSSEIKQFFGDKYSDVVRVVSAGHSHELCGGTHAEATGDIGFFRITKEHAVAMGIRRIEAVTGEKAEATVHKELEKVAKVKSELEMKTAEIRRRYLAHFEANGHAVVPSAPLPAISDPNLLFVAAGMMQFVPYFLGQQTAPYKRAVSVQKCLRTPDIDEVGKTSRHGTFFQMNGNFSFGDYFKDEAIPLAWELSTKPVDAGGLGLDPDRIWPTVYLDDDEAFQIWRSVGVPADRIVRRGKADNFWSMGIPGPCGPCSELFYDRGPEYGREGGPEVDEDRYLEFWNLVFMQFERGPGTGKEDYPILGDLPAKNIDTGMGLERMASILQGVDNLYEIDEVRPILAKAAELTGKRYGAHSGQVASESHPDDVRLRVVADHVRTALMLIGDGVIPSNEGRGYVLRRIMRRAIRSIRLLGWQERAMPELLPVARDCMSASYPELATDFGRIADYAYAEEDAFLSTLRAGTTILDTAIAETRTAGRRAISGAKAFQLHDTYGFPIDLTLEIASEQGLQVDAEGFRRLMADQRARAKADAQARKTGHVDLSAYRTVLDEGGPVTFTGYQEVSRESTVRAVLGAASPHAAAVEGETVELVLDTTPFYAEGGGQQPDLGVITVGGGQVEVLDVQQPVPGLIVHRARVLRGEVRVGETGFAEIDTDRRRAISRSHTATHLVHQTMRNFLGESATQAGSLNAPGRLRFDFNTPTGVAPSVLRDVEQQVNEALLADLEVRAFVTSLAEARRIGAMALFGEKYGEQVRVVEVGDYARELCGGTHVGRSGQLGLVKILSESSIGSGVRRVEALVGIDAFNFLA"
  },
  {
    "reference": "MPIVDSGSVSPLTAADKTKILAAWDLVYKNYEKNSVDILVKFFTGTPAAQAFFPKFKGLTTADDLKKSSDVRWHAERIINAVNDAVKSMDDTEKMSMKLKELSNKHVKNFNVDRKYFKVLAGVIADTVAPGDASFEKLMSIICILLNSAY",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a binding site that is specific to the target heme b ligand.2. The protein must be able to oxygen carrier activity, iron ion binding, heme binding. The designed protein sequence is ",
    "response#1": "VSMADLIVLAGCAGVEQAAKAGGHQIEVPFTSGRGDASAEQTDAESFAVMEPVMDGFRNYQARELSTSPEEMLVDRAQLLGLSAPEMTVLVAGLRVLGANHGGSAHGVLTNRPGVLSSDFLTNLLDMGTEWKPSGKGVYEGRDRASGAARWTATRVDLVFGSNSQLRALAERYAQDDAEASFVADFVAAWVKR",
    "response#2": "VSMADLIVLAGCAGVEQAAKAGGHQIEVPFTSGRGDASAEQTDAESFAVMEPVMDGFRNYQARELSTSPEEMLVDRAQLLGLSAPEMTVLVAGLRVLGANHGGSAHGVLTNRPGVLSSDFLTNLLDMGTEWKPSGKGVYEGRDRASGAARWTATRVDLVFGSNSQLRALAERYAQDDAEASFVADFVAAWVKR",
    "response#3": "VSMADLIVLAGCAGVEQAAKAGGHQIEVPFTSGRGDASAEQTDAESFAVMEPVMDGFRNYQARELSTSPEEMLVDRAQLLGLSAPEMTVLVAGLRVLGANHGGSAHGVLTNRPGVLSSDFLTNLLDMGTEWKPSGKGVYEGRDRASGAARWTATRVDLVFGSNSQLRALAERYAQDDAEASFVADFVAAWVKR"
  },
  {
    "reference": "MIQVAGVNFEDPIVIASGIVPPTKEYMQNVCEKYEPSAITSKTLTYSPLEPHRSPTFVKISDNCYLNAIGLGNPGIQILRDLGEIKCKLIISIGGSNVNEYIDAVSKINDIPVMIELNVSSPNRRGFGESNLTYVEEIVKNVKSIVKKPVFVKLGPWDNIVEIAGRALSAGADGLTLINTLKGMLIDVEDFKPILSYGTGGISGKCIHALAVRVIHDVFKEYEPEIIGVGGVFDWRDAIELISVGAKLVGLGTVLVEKGFDVIREIREGIGTYLEEKGLKVEEIRGIGVKR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. We require a protein that can specifically bind to the metabolites produced in the orotate from (S)-dihydroorotate (NAD(+) route): step 1/1, Pyrimidine metabolism pathway.2. The designed protein should have a high specificity for FMN, substrate binding, with minimal off-target effects.3. The desired protein should be able to perform (S)-dihydroorotate + NAD(+) = H(+) + NADH + orotate4. The designed protein should have dihydroorotate dehydrogenase (NADH) activity within cytoplasm to regulate 'de novo' UMP biosynthetic process, 'de novo' pyrimidine nucleobase biosynthetic process. The designed protein sequence is ",
    "response#1": "MLAQDVAGIHFTNPVGLAAGFDKSCEVPKALQYSGFGALELGGITPKPQPGNPKPRMYRLPEDRALINRMGFNNIGMNKALSNLRRHSYELPIGINVGVNKMTAYEERYQDYIKVLDTFKNDATFFTINISSPNTANLQNFHDKDEFSMLTKAIKDYRENHELSQPIFLKLTSDLELEGFKHILDPIVDTFDGVVLANTTQQRDGLQSENRSEGGGLSGRPLFERHRDLVRWAYQQTKGTFPIIATGGIFSAEDAIEMMRNGASLVQIYSSLVYEGPGLTAKINKDIVKAYHK",
    "response#2": "MLAQDVAGIHFTNPVGLAAGFDKSCEVPKALQYSGFGALELGGITPKPQPGNPKPRMYRLPEDRALINRMGFNNIGMNKALSNLRRHSYELPIGINVGVNKMTAYEERYQDYIKVLDTFKNDATFFTINISSPNTANLQNFHDKDEFSMLTKAIKDYRENHELSQPIFLKLTSDLELEGFKHILDPIVDTFDGVVLANTTQQRDGLQSENRSEGGGLSGRPLFERHRDLVRWAYQQTKGTFPIIATGGIFSAEDAIEMMRNGASLVQIYSSLVYEGPGLTAKINKDIVKLQVNLGGLTMKNPVTTASGTFGFGLEFAPFIDLSRLGAIVVKGTTLEPREGNPTPRMAETPAGMLNAIGLQNPGVEAFIRDYLPPLRQIDTPVIVNVSGNSVEDYGRLASILDEADGVAALEVNISCPNVKEGGIQFGTLPASAAAVTRAVRENTQKPVIVKLSPNVTDITEMARAVVDAGADALALINTLLGMDIDICTRRPVLANIFGGLSGPAIKPVALRMIYQVYRAVHVPILGMGGIMNSQDAIAFMLAGATAIAVGTANFTNPRATMDILEGIEEYCCREGVRDVNELIGAAKKL",
    "response#3": "MLAQDVAGIHFTNPVGLAAGFDKSCEVPKALQYSGFGALELGGITPKPQPGNPKPRMYRLPEDRALINRMGFNNIGMNKALSNLRRHSYELPIGINVGVNKMTAYEERYQDYIKVLDTFKNDATFFTINISSPNTANLQNFHDKDEFSMLTKAIKDYRENHELSQPIFLKLTSDLELEGFKHILDPIVDTFDGVVLANTTQQRDGLQSENRSEGGGLSGRPLFERHRDLVRWAYQQTKGTFPIIATGGIFSAEDAIEMMRNGASLVQIYSSLVYEGPGLTAKINKDIVKRL"
  },
  {
    "reference": "MRVGILTSGGDCPGLNAAIRAVTRRLIAKNDFEVIGILNGFKGLIEGNIEPLSLYSVSGILPKGGTILGTSRVNPFNEKGNVERMQKTMQRYELDALVVIGDGDALNVAHQLETSGIHSVCIPTTIDNDVAETDRTFGFDTAVTIATEAIDRLHSTADSHHRIMVVEVMGRQSGWIATMAGLAGGADYILIPEVEAPLDKISELLTRRHKRKKFSIVVVAEGARPEGMPESGPKGKKTDEFGHWIPRGVGEYIGQELEKRTEMETRVTILGHIQRGGTPTAYDRVLASRFGVKAADMVASGKFSTMTALKGSKIVEVPLENVVGKIKPVDPELYEIASVFFG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein can be expressed in cytoplasm and perform 6-phosphofructokinase activity, diphosphate-fructose-6-phosphate 1-phosphotransferase activity, metal ion binding without triggering undesirable cellular responses.2. The designed protein should contain one or more Phosphofructokinase domains that are essential for its function.3. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis; Consistently, PPi-PFK can replace the enzymes of both the forward (ATP- PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions..4. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in beta-D-fructose 6-phosphate + diphosphate = beta-D-fructose   1,6-bisphosphate + H(+) + phosphate are properly positioned for optimal catalysis.5. The designed protein should have a high specificity for substrate, diphosphate binding, with minimal off-target effects.6. The protein design should consider the endogenous proteins and enzymes present in the D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 3/4, Carbohydrate degradation pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "LLVVHGGGPTAVLNASLYGVIREAQKNPEINKIYGAIGGSEAVIEENFLDMGELSDEKIELLLQTPGTAIGSSRFPLEQEQYEAMVAVLKKNNIKYVLFNGGNGSMDTCGKVSQACEGEGIYVVGIPKTMDNDLSMIDHAPGFPSAAKYIATVTKEVGADVKSLPIHVCVIEAMGRNAGWITASSALAREKAGDAPHLIYLPERNFNEEEFLEDVKKLYDELGGVVVVVSEGLRNEKGESIVPPIFKTERAIYYGDVSAYLAELVIKKLGIKARSEKPGLCGRAFHWSDEVILEKINVGIILSGGPAPGGHNVISGLYDGLKTNNKDSRLFGFILGPGGLINHEYKELTADIIDEYRNTGGFDLIGTGRTKLEKEEQFEKGLEIMKKLDIKALVIVGGDDSNTNAAILAEYYQAKQAGIQVIGCPKTIDGDLKNDIVEASFGFDTTCKIYSEIIGNIERDCNSTRKYWHFIKLMGRSASHIALECALQTQPNICIISEEVAAKNQTLDDIVTYIAKAVAERAERGLNFGTVLIPEGLIEFIPAMKSLIAELNDLLADTTEGQTAKQSEQRNWVISRLSTKNAAIFASLPESVAAQLTLDRDPHGNVCVSRIETEVLLSSMVGMKLAQWKAEGKYVGKFATQHHFLGYEGRCGFPSNYDADYCYALGCTAAQAILNGYSGFMAALSGDDVIRVPLEEAVRGGKRVYKEWERIARS",
    "response#2": "MNRVGILTGGGDCPGLNAVIHAVVKKGIQHYGDEFVGFVEGWRGVLDNNTVALTLEKVDGILDQGGTILRTSRTNVRKIEGGIEKCVETLKANKLDALIAIGGDDTQSVTNALIQAGVPGVGVPKTIDNDLNGTDACFGFDTAVSIATEAIDRLHTTAEAHNRVIVCEVMGRDAGWIALTAGVAGGAHAVLVPEKQIDLDHVCKLLKYNHDHGKKYGIVVVAEGAKLPDSGAQATHGTKVDSFGHARLSGIGSILADEIEKRTGYETRSVNLGHTQRGGTPTAYDRMLSTRYGLAAIDAVNAGAWGTMVALRGDTIVSVPMEEAVGDLKTVDQALYDEAEVFFG",
    "response#3": "MKIVGVILSGGQAPGGHNVIAGLFDGLKKLNPKNKLYGFLDGPSGLVDHKYVELTSEIIDEYRNTGGFDIIGSGRTKLEQEWQFEQGAEICTKLKIKAIVIIGGDDSNTNACVLAEYYAQKNYPIQVIGCPKTIDGDLKNEMIETSFGFDTACKVYSELIGNIQRDASSAKKYWHFIRLMGRSASHITLECALQSQPNITLISEEVEGKNLSLNDIVEYIVKIIVNRANYGVNFGTVLIPEGLIAQAGGVTAVINVSAAGVIETLRQYNTPRIYAAINGIQGLLDEELVDLTQINDAELERLKCLPGGAFQACRFDLDTLEKRPDQYERLLQIFSYYDIGTFFYNGGNGSMLTAQKVADYCQSKGYPLRCIGVAKTIDNDLAMSYCSPGFGSAAKFLASHFLQATLDIQSMSASSTKFFVMEAMGRNVGWLALSAGLVKDVIADLPLIILPAERAFNEQRFLHKVSQLIDKHGYCVCMASEGLCDTEGQCLSVAREEHAHGKVYRQFGGVAQKLAQLVEQALQVKTHSANPDYLQRSSALCVSATDWQMAYEAGAAAVHIGILTGGGDCPGLNAVIRAVVKTAHLYDYSVVGIRNGWKGIVDGEVEPLTEFAVTGILPKGGTILGTSRTNPLKSEEHTQQMLANLKRFGIDAVVAVGGDDTLSVATRLHQMNVPVVGIPKTIDNDLSGTDYTFGFDTAVSIVTDAIDRLHTTAESHHRIMVLEVMGRHAGWIATMAGIAGGADQILIPEVPFDIDDVCDGLKKRQDRGKKFSIVVIAEGAKPTDMSGFATISSKVDEFGHEQLGGIGHMLGRAIEERIHSETRVTVLGHVQRGGSPTAHDRVLATRYGVKAVE"
  },
  {
    "reference": "MLAMASGVSLAVELALLLGLDKLPEFEENAPTFAENAAGKALHYSRLWDGLVFADDSGLVVPALGGAPGVHSARYAGPRATNSQRIEKLLGEMRGKTGAERAAYFVCAIALTEHGRAKAIVTDRVEGEILEAPRGSGGFGYDPVFYFPALGKTFAEIPAEEKNQRSHRGKAFRRLLSSLSSML",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must bind to Mg(2+) in order to perform its enzymatic function.2. A protein with XTP diphosphatase activity functions is required to enable nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "GRHIVVPLALIVKGGKILMNKRNDPHVPQFHGVWEFQGGSVEYGETIEENVRREAKEESGYEVEVAEYLPTIEVKNRQYNDMRIQLYLVPIVCRIIRGDGKYNDQEVLEAKWYALDDVPNLNLFPGDGEMYRKFLY",
    "response#2": "GRHIVVPLALIVKGGKILMNKRNDPHVPQFHGVWEFQGGSVEYGETIEENVRREAKEESGYEVEVAEYLPTIEVKNRQYNDMRIQLYLVPIVCRIIRGDGKYNDQEVLEAKWYALDDVPNLNLFPGDGEMYRKFLY",
    "response#3": "GRHIVVPLALIVKGGKILMNKRNDPHVPQFHGVWEFQGGSVEYGETIEENVRREAKEESGYEVEVAEYLPTIEVKNRQYNDMRIQLYLVPIVCRIIRGDGKYNDQEVLEAKWYALDDVPNLNLFPGDGEMYRKFLY"
  },
  {
    "reference": "MSTSEFVLAQSATVPAPDIEYGQLSPMLIVFGVAVAGVLVEAFLPRRGRYSSHLVLALGGLTAAFVAVVLLAGTRDSVVGGAVAVDGPTLFLQGTILLISIPAILIIAERSVDSGVAAATAEVTAVRGAEPEGGTDAFTPQAFAVPGSVAEREATRNAPGHTEVFPLTLFAVGGMLLFPASNDLLTMFVALEVLSLPLYLLCGLARRRRLLSQEAALKYFLLGAFSSAFFLFGVALLYGYAGTVALPGIADALARGTEDRTLALIGTALLSVGLLFKIGAVPFHSWIPDVYQGAPTPITAFMAAATKVAAVGAMLRIFYVALPDLRADWRPVMWGVAILTMVVGAVMAVTQNDVKRMLAYSSVAHAGFILTGLVAANRAGLSSTMFYLLAYGFSTLGAFAVVTLVRDSRGEATDLSRWAGLGRRSPLVGGVFALFLLAFAGIPLTSGFVSKFAVFQAALEGGAVPLVLVGVVSSAIAAFFYVRVIVLMFFSEPQSDAPTIVVPSMFTAAAIAVGVVVTVVLGILPQGALDLADQAAVFFR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.; The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone; NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain2. The desired protein should be able to perform a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) +   NAD(+)3. The protein should contain a Helical membrane-spanning region.4. A protein that can perform quinone binding, NADH dehydrogenase (ubiquinone) activity in plasma membrane to support ATP synthesis coupled electron transport. The designed protein sequence is ",
    "response#1": "DMGAWQTPLAFGMREREKILDLFEMASGARLMCNYFRFGGVVRDLPPEFIPNLRELLIALPAYIDELERLLRDNEILLQRAIGIGILPREVALSYGVTGPVLRGSGIPYDVRKAEPYSVYDRFDFDIPVGKVGDVYDRFLVRIEEMRQSKRIIEQVLEQLPDAKGGHINPKARQNVRPPVGDAYARIESPKGELGFYLVSDGSGKPYRYKVRAPSFINLTPLADMCRGYKVADIVVILGSIDIVMGEVDGE",
    "response#2": "DIGAMTVFLYAFREREQILQFFEKICGARLTVSYPRIGGVRVDIKEHVLDEIYKFMDLMLIRVDEYETLLTENRIWIARTRGVGVIAPEDAVLLGLTGPALRGSGVYYDIRKQIPYDAYSEIDFEVPLGEKGDTYDRYLCRIREMRQSVLIVKQCIEKMPEGKILSDKSPDIDLPHQAKRKIEPGDSLWNGFIAFSEEKQEIMPKGEIYSAIEAPKGELGFYIVSDGSGRPYRMRVRAPSFIHISAIPKLCEGHLLADVIAIIGTLDIVMGEADGE",
    "response#3": "LGALTVMTIGFRERELVLDLFELITGLRMNHAFIRPGGVAQDMPPGALDEIRGFVALMKKRLPEYADLCNANPIFKGRLEGIGHLDLAGCLALGLTGPVLRSTGYPWDLRKTQPYCGYETYDFDVQTWDTSDSYGRFRIRLNEMWESLRIIEQAADRLAGLDGAPVMIEDKKIGWPSQLAIGSDGMGNSLDHIRHIMGESMEALIHHFKLVTEGFRVPPGQAYVPVESPRGELGAHVVSDGGTRPFRAHFRDPSFTNLQATSVMAEGGMVADVIVAIASIDPVMGGVDQVLRRQTDLGAFTPFLFCFRDREHLLNLLEWICGARMLYNYIWVGGVSHDFPAKFEERVMEFVRYFKPQARELYQLLTENEIFVQRTKDIGLLPADVAINAGASGPMLRGSGVKWDLRRDDPYSIYPELEFDVAVPDGKASVIGDCLSRHLVRAIEIDESLKMIEQCIDMMPKDPNFDPHQH"
  },
  {
    "reference": "MMTTDNISAAPAPVNLLGYDRARLEAWFESIGEKKFRAAQVMKWVHQLGVADFDEMSNISKALREKLKRIAVIRGPEVVYREFSADGTRKWVFKVGENSMVETVLIPDGDRKTLCVSSQVGCALDCSFCSTGKQGFQRDLSAAEIIGQVWVANRSYFEDVPDYDARERAITNVVMMGMGEPLLNFDNVVPAMDLMLDDYAYGLSKRRVTLSTSGVVPMMDRLTQTIDVALAVSLHAPNNTLRDELVPINRKYPLEDLMAACRRYLHHYQGDFGRRKITMEYVMLDGVNDHPEHARQLIRLLKDVPSKINLIPFNPFPHAPYGRSPRSQILAFQKLLVEAGYICTIRATRGDDIDAACGQLVGQVQDRTRRFEKWQQAVKAGEILRSPQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have metal ion binding to facilitate rRNA base methylation. The designed protein sequence is ",
    "response#1": "EGEPPYRSGQVWRALHQGLQPLEMTDLPGPLRRRLAAALPPGLDLSATAVSEAGDTVKWAWGLHDGTAVETVLMHYPGRATVCVSTQAGCAMACSFCATGQAGFRRHLTAGEIVEQVVRARRAALPRRLSNVVFMGMGEPLANYGATWAAVERIHDELGLSARHLTVSTVGLVPGILRLADESLPVNLAVSLHAANDELRDTMVPINRRWPLPVLMDACRTWREAKGRRLSFEWALIDSVNDRPADAGELAALATPLAAHVNLIPLNPTPGYATRGTPPAGVRAFRDRLRHLGVNATVRRNRGTDIAAACGQLARV",
    "response#2": "SSQVGCALGCTFCATGQQGFTRHLSSGEIISQVLYYKQLDNKGFFDKVLDKPEKSYKGIKNIVFMGMGEPLANYENTINAIRILNDSQGTNFGARNITVSTVGLVPQILKLADEKIQINLAVSIHAPDNETRSQTVPVNKRYPIEMLIDACKEYIKKTNRKIFFEYVLLNEQNR",
    "response#3": "EGEPPYRSGQVWRALHQGLQPLEMTDLPGPLRRRLAAALPPGLDLSATAVSEAGDTVKWAWGLHDGTAVETVLMHYPGRATVCVSTQAGCAMACSFCATGQAGFRRHLTAGEIVEQVVRARRAALPRRLSNVVFMGMGEPLANYGATWAAVERIHDELGLSARHLTVSTVGLVPGILRLADESLPVNLAVSLHAANDELRDTMVPINRRWPLPVLMDACRTWREAKGRRLSFEWALIDSVNDRPADAGELAALATPLAAHVNLIPLNPTPGYATRGTPPAGVRAFRDRLRHLGVNATVRRNRGTDIAAACGQLAR"
  },
  {
    "reference": "MNDTTLVLLGAGNSTRFGLKTKKQWLYIGEEPLWYFVTKQFKKYGFKETIVVSSIQEIPYMKKFDTFAFVEGGETRQESLKNALQNVLTKYVLVSDIARCCIDHDMIQRILDNKEKANCIVPALSISDTLYMDDSPANREGAKLIQTPQLSETKLLVKAIQTDELFTDDSSAMHKSGYKILFVEGSQNAHKLTFASDLEKLTCLKAPSLKTFTGFGIDTHPFEQNKPMYLCGEKIEENFGFKAHSDGDVAIHAIIDALLGAAGLGDIGEFFPDSDDKYKDIDSKELLKLCVEKVNSYGFAINNIDITILAEIPKISPYKEKMRQTLSGILDINQRSVNIKATTSESLGFIGRNEGISVHAVATLSYFNWAEI",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must be able to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity.2. The presence of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domains is critical for the interaction of the designed protein with its intended targets.3. A protein that is capable of catalyzing reactions within the isopentenyl diphosphate biosynthesis via DXP pathway, isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6, Isoprenoid biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "VRMGNGYDVHAFCDGDHVWLCGIKVPHGRGLLGHSDADVGMHALTDAIYGALAEGDIGRHFPPSDPQWKGAASHIFLAHAVELARSRGYDLGNCDVTLVCERPKIGPHALAMQAELARIMGVAAGRVSVKATTSERLGFTGREEGIAALATAVLVL",
    "response#2": "RVGQGYDVHAFTDGDHVTLCGVRIPHDRALLGHSDADVGMHALADAIYGALAEGDIGRHFPPSDMRWKGAASEVFLAHAADRARARGYRVGNADLTLVCESPKIGPHAAEMQAGIGRILGVAPDCVSVKATTSERLGFTGRGEGIAAMACVTVRTGTGFDVHRFGPGDHVTLCGIKIPYSHALMGHSDADVALHALTDAILAALADGDIGSHFPPGEADWAGMDSAHFLRFAAGRVAARGGLIAHLALTLICERPKIGPHRASMAARIAEIAGIGPARVSVQATTTEKLGFTGRGEGIAAQAAATVRL",
    "response#3": "VRMGNGYDVHAFCDGDHVWLCGIKVPHGRGLLGHSDADVGMHALTDAIYGALAEGDIGRHFPPSDPQWKGAASHIFLAHAVELARSRGYDLGNCDVTLVCERPKIGPHALAMQAELARIMGVAAGRVSVKATTSERLGFTGREEGIAALATAVLVLA"
  },
  {
    "reference": "MNRIFKNLAVYLLIVLLAVSIIRLSTPTERTIEEWDLTRFFQAVDQGQVQEVTLTPHDSIIKIDGILKNNTRFTVNAPASVNLIDRLTAKNVTVKAQLPPQPPWWTNLLGSLLPILLLVGLVVFMMQQTQGGGSRVMQFGKSRARLHTDEKRKVTFNDVAGADEAKEELEEVVEFLKNPRKFNELGARIPKGVLLYGPPGTGKTLLARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKNSPCIVFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFSANEGIIIIAATNRPDILDPALLRPGRFDRQIVVDVPDVVGRKEILKVHVRGKPLAEDVDLDVLARRTPGFTGADLANMVNEAALLAARRGKKKIGMDEMEDAIERVIAGPEKKSRVISDYEKRLVAFHEAGHALLGHYLPHTDPLHKVSIIPRGRAGGYTLLLPKEDRRYMTKSQILDQVTMLLGGRVAEALVLKEISTGAQNDLERATELVRKMITEFGMSDELGPLTFGRRQETVFLGRDIARDRNYSEAVAFSIDKEARHIIDECYNRAKEILQKHMDELHLVARALMEKETLEAEEFTAIIEAYNQEHRPQEDNSKLPEGDNKPPEAGGGTPAKEPVIKLNYFHCLKGVW",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+), ATP binding.2. The designed protein must have a active site that is structurally stable and resistant to denaturation.3. The designed protein must have at least one AAA+ ATPase domain.4. The designed protein should have at least Helical transmembrane domains to ensure proper localization.5. The ideal protein would be one that plays a critical role in protein catabolic process, proteolysis through ATP hydrolysis activity, metalloendopeptidase activity, zinc ion binding, ATP binding, ATP-dependent peptidase activity within plasma membrane.6. For general function, the protein need meet that Plays a role in the quality control of integral membrane proteins.; Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins7. The protein must bind to Zn(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MVMSDDEKKMTAYHEAGHALVAMHEPECDPVHKATIIPRGRALGLVMSLPAGDRYSKHKSKLKAELSMAMGGRVAEELIFGADKVSNGASGDIKMATNQARMMVTEWGMSEKLGMIAYSDNSQEVFLGHSVTQSKNVSEATAREIDSEVRSIIDEAYARAKHTLQTNIEELHALAKGLLEYETLSGDEIRTMLVERDRMVASIPRGILLVGDPGCGKTLLAKAIAGEAEVPFFSISGSDFVEMFVGVGASRVRDLFKQAKENSPCIIFLDEIDAVGRRRGANFSGGGHDEREQTLNAILVEMDGFETSDQVIVIAATNRVDVLDPALIRPGRFDRQVFVALPGVAYDVDPKIIARGTPGFSGADLANLVNEAALLAARSGRRTVGMHEFESADVERAVEMLIAEE",
    "response#2": "VLLVGPPGTGKTLLAKAIAGEAGVPFFSMSGSEFVEMFVGVGASRVRDLFRKAKESAPCIVFIDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNSGVIIIAATNRPDVLDSALMRPGRFDRQITVLFLGVPMSILLERVDLSIIARGTPGYSGAELANLLNEAALLAARTNKKGVGMKELERVDLEEIARSTPGFSGADLHNLLNEAALIAARYKRKSIEQQDIQRCVDWTLRXELLKXGTXFGXHXGXGXGXGSXHXDXYXFXQX",
    "response#3": "MVTPKEKRIVSVHEAGHAVVAYALPDADPVHQVTIIPRGGAGGYTMRLPTDERNYMTKNQMLNELKIMLGGRAAEKIILKDISTGASSDLKSVSSLARNMVVKYAMGESLSQMAYSEEDNVFIAKDMTTKRDLSEQVVAEIDREIRTLVDTSYDETIRILESRRNQLEDVAKALVEFETIDADEFLAAAAASRRYRRRTGIG"
  },
  {
    "reference": "MPARPEDTPADVDVRRRVESAVAALASGRIVVVADDEDRENEGDLILAAEFATPENIAFMVRHTSGLLCVGVPPERTESLGLPLMVDDGDDPRGTAFTVSVDVRHGTTTGISSEDRAATARALADPATVPADLIRPGHVFPLRARPGGVLQRAGHTESAVDLCRLAGLQPAGVLAEITNDDGTMARMPDLERFASEHGLVLLSVADLVRYRRTCETLVQRQATSRVPTEFGEFQATSFRSAIDGREHLALVLGDVDQCQGGTLADVPVLVRVHSECLTGDVFGSRRCDCGEQLRAALANIAAVGRGALVYLRGHEGRGIGLGHKLRAYALQDTGLDTVDANLAQGLPIDSRDYGIGAQILGSLGISTVRLMSNNPAKYRGLSGHGVFIVERVPLLVTPNCDNVQYLKTKRDRMAHLIAEIDVS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Nucleophile; for GTP cyclohydrolase activity active site of the designed protein must be optimized for substrate binding and specificity.2. The protein must be able to 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, zinc ion binding.3. The GTP binding site should be located in a region of the protein that is accessible to the ligand.4. Ideal protein should have a binding affinity for key enzymes in the 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "RVAEAKLPTKFGDYTAIAYKSDIDPNEHVALVMGDITSPEPVLVRVHSECLTGDVLGSMRCDCGEQLQIAMQRISKEGRGVILYMRQEGRGIGFHNKLRAYELQDNGLDTVEANIALGFAPDLREYGIGAQILVDLGLHEIRFLTNNPKKIIGFEGYGLKVVEQVG",
    "response#2": "RVASVHLPTDFGDFTAIGYANDVDDKEHIALVKGEIDPEKPVMVRVHSECLTGDVFGSHRCDCGPQLHAALSQIDREGTGVLLYMRQEGRGIGLLNKLKAYELQEKGLDTVEANLKLGFRPDLRDYGIGAQILRDLGVRKMRLLTNNPRKIAGLKGYGLEIVEVLG",
    "response#3": "VRVSMPTKYGTFELIAFKQTNTGEEHMALKKGKWEKDEPILVRVHSSCMTGDILGSLRCDCGDQLHNAMKMVEAEGKGVVLYMNQEGRGIGLLNKLRAYKLQEDGRDTVEANLELGFGMDQRDYGIGAQILRELGITKIKLISNNPRKRAGLLGYGLEIVDTVAFRG"
  },
  {
    "reference": "MSVLSGKKIVLGVSGGIAAYKTATLVRLFIKAGAHVQVIMTPASKDFVTPLTLSTLSKNPVHSSFYNEEDENAQWNNHVEIGLWADLMVIAPATANTMSKMVNGNCDNLLVATYLSAKCPVYFAPAMDLDMYKHPSTIASFNALQNFGNTIIPAESGELASGLSGEGRMAEPENIVAFIEADLESKLPLKGKKILITAGPTYEAIDPVRFIGNHSSGKMGFDIANCAANLGAEVILVSGPTHFKVKNDFINLVRVVSAQEMYDACHQYYNDVDVAIAAAAVADYRPKVVADQKIKKSDTCFSIELEKTKDILASLGEIKKNQFLIGFALETENEIENAKLKIQKKNLDLIVLNSLQDEGAGFGKPTNKITFIDSNFTIEPMDLKSKEDVAQDIINKVIEHYHA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.2. We need a protein that can perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2 with high efficiency.3. The protein contains novel DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains that confer a unique function or activity.4. The designed protein should have phosphopantothenate--cysteine ligase activity, FMN binding, metal ion binding, phosphopantothenoylcysteine decarboxylase activity to facilitate pantothenate catabolic process, coenzyme A biosynthetic process.5. The protein should be able to bind CTP ligand in a variety of conditions6. The designed protein should interact with enzymes and substrates within the Cofactor biosynthesis, coenzyme A biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MSGGIAAYKACHLVRDLKEAGDDVRVVPTPSALNFVGAATFEALSGHPVSTTVFDAVDEVQHVRVGQEADAVVIAPATADVMARLVAGRADDLLTATVLVAQCPVIVVPAMHTEMWWNPATQANVRTLRERGVIVLEPAHGRLTGRDTGPGRLPEPDQIAELAAAVLAQRRDLLAGRRVLVTAGPTREAIDPVRFVSNRSSGRMGYALAEVAWRRGAAVTLVSGPTALPPPVGVELVRVETAAAMAEAVAAHLPAADVLVMAAAPADFRPAAPAAAKLTKEDGLPPPLAWALTADILQSTRPLRRPDTRVVGFALETADDALARARAKLEAKGLDLVVLNRADEADAGFDTETNRVTVLWPDGQRELPRQSKQELARVLLDRVAALLVAGGP",
    "response#2": "MSGGIAAYKACHLVRDLKEAGDDVRVVPTPSALNFVGAATFEALSGHPVSTTVFDAVDEVQHVRVGQEADAVVIAPATADVMARLVAGRADDLLTATVLVAQCPVIVVPAMHTEMWWNPATQANVRTLRERGVIVLEPAHGRLTGRDTGPGRLPEPDQIAELAARLLGEPSLAGRRVLVTAGPTREAIDPVRFVSNRSSGRMGYALAEVAWRRGAAVTLVSGPTALPPPVGVELVRVETAAAMAEAVAAHLPAADVLVMAAAPADFRPAAPAAAKLTKEDGLPPPLAWALTADILQSTRPLRRPDTRVVGFALETADDALARARAKLEAKGLDLVVLNRADEADAGFDTETNRVTVIGADREIAEFPLKPKSQVADAVWDLVVPLLR",
    "response#3": "MSGGIAAYKACHLVRDLKEAGDDVRVVPTPSALNFVGAATFEALSGHPVSTTVFDAVDEVQHVRVGQEADAVVIAPATADVMARLVAGRADDLLTATVLVAQCPVIVVPAMHTEMWWNPATQANVRTLRERGVIVLEPAHGRLTGRDTGPGRLPEPDQIAEVARLLLGSDLAGRRVLVTAGPTREAIDPVRFVSNRSSGRMGYALAEVAWRRGAAVTLVSGPTALPPPVGVELVRVETAAAMAEAVAAHLPAADVLVMAAAPADFRPAAPAAAKLTKEDGLPPPLAWALTADILQSTRPLRRPDTRVVGFALETADDALARARAKLEAKGLDLVVLNRADEADAGFDTETNRVTVLDRAGEVVALEGRRVVISAGGTRESLDPVRYLGNRSSGRQGYALARIAAHRGADVTLVAAHTADLITPAGVRLIRVGTADEMRSVMLTEADGADAVVMAAAVADFKPVNRVEHKIKKTDRDPEPVALSRNPDILAEIVEARSAGKLSASLIVGFAAETGDATSSVLDHGRAKLARKGCDMLVVNAVGEGMAFEVEDNSGWLLGADGTESAVPHGSKTALAHAVWDAVVGLL"
  },
  {
    "reference": "MEKIDINNFTLEELEEYVVSLGEKKFHAKQIFKWIHKLKVIDFDEMTDISKSLREKLKENTYILTMNVIKCQISQKDGTMKFLIGLNDGCAIESVFMKYNHGNTLCVSTQVGCKMGCKFCASTKEGFERSLRASEIEGQILTVERYTNEKISNVVYMGIGEPLDNYDNTVKSIRIINNPQGLNIGARHISLSTCGLVPNIYRLADENIQCTLSISLHATTDEKRRDIMPIANRYSIDELLNACKDYIKKTGRRISFEYALISGKNDSYEDALALAKLLRGMIAHVNLIPVNEIKEKDYKKAKEKNVEKFMNTLNSMGIVTTVRRELGSDIDAACGQLRKKYIENSNKL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be designed to catalyze the following reaction adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.2. The S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein must be optimized for tRNA (adenine-C2-)-methyltransferase activity, rRNA (adenine-C2-)-methyltransferase activity. The designed protein sequence is ",
    "response#1": "QHYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLTSGEITAQIMLVQKYFDQREQNERVSHVVVMGIGEPFDNYDNVLNFLRIINDDRGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVSLHAPNNELRSSIMRINRSFPLEKLLGAIEYYIDTTNRRVTFEYIMLNRVNDWPENAQELADLTKKIRKLSYVNLIPYNSVSEHDQYSRSPQDRVAAFYDVLKKNGVNCVVRQEHGAE",
    "response#2": "MATPLLGASLDELTNWVQEQGQPRYRGKQLYEWIYDKGAKSLEEITVFSKQWRETLQETPIGRSQIHYRSVASDKTVKYLLKLADGQIVETVGIPSSDRLTVCVSSQIGCPMACDFCATGKGGFQRNLAAHEIVDQVLTVQEDFKRRVSHIVFMGMGEPLLNTDNVLKALKCVNQDVGIGQRSMTVSTVGIPGKILKLAKHKLQVTLAVSLHAPNDKLRQQLIPSADRYPISDLLSECKEYVNQTGRRVTFEYVLLDRVNDYAQQAFELASLLRGFQSHVNLIPYNPIADADYQRPSPQRVKAFADTLRDRNITASIRYSRGLEADAACGQLRANQE",
    "response#3": "SSQIGCSLDCSFCATGKQGFNRDLTAAEIIGQVWIAAKSFGSLGPKAERVVSNVVLMGMGEPLLNFDNVVDAMSLMLHDNAYGLSKRRVTLSTSGVVPMLDRLGDVIDVSLAISLHAPNDKLRNELVPINKKYPIAMLLDSARRYLDKMPDKRRKITIEYTLIDQVNDRNEHAHRGEMPDNVPEVQSVLERGGEPAYRLRQAYAALTGSLVRDWEEATSLPQGLRGALNQEAPAAVLDLRRTWRATDGTRKYLFHTHDGHAIETVMIPEKGRHTVCISTQVGCAMACKFCATGLLGIKRNLGAREIAEQVFVCARDISPERVSNVVVMGMGEPMLNYKATLGALKVLNDPKGFGLAARHIAVSTSGLVDKIRRFADEPEQFHLAISLHTPFEEERREIMPVAARHTIPELMDAARYFVSRTRRKLFFEYTLLAGVNDQPRHVEALAELFDHPLYHLNLLRFNWTDTGFSATSKADAKRFLNHARDLGLSATLRPSRGQDIDAACGQLAARDLQTEM"
  },
  {
    "reference": "MQVDLKNKTILLGVTGSISAYKACDIARLFVKAGAQVHVVMTPSAERFVSALTFEALTRNPVLTESSESWVSELNHIEIGKKCDTFLIAPATANTINKLSKGIADNLLTQTALAYAGPMLVAPAANTQMLKNHYTAGSLKMLGVNDYTIIQPQEKLLACGDVGSGALADPSEIFFETAKALLKENFWEDRRIVVTGGGTREKIDEVRYISNFSSGKMAKALCLSLYLKGADVCYITTAGKEGLPQNIYTIEVDDTKELLDYTQDAVRVAKKGKMSKVSLNSYDPRHLIQKEPYLFMVAAVSDFTPRYPQKGKLKKSLLGNTWSLELKQTPDILSVLNKEGIKTIAFKAEMDPEHGLANAAALLEQKEVDGVCYNLLNNSESFGTEENEITFITKESQTTLERTDKLTLSGYILDKAKALIDG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should contain one or more Flavoprotein domains that are essential for its function.2. A protein with phosphopantothenoylcysteine decarboxylase activity is needed to enhance coenzyme A biosynthetic process in a specific context.3. A key design requirement for the protein is the optimization of the Proton donor to achieve high catalytic efficiency for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. A protein should be able to manipulate the activity of enzymes in the coenzyme A biosynthesis pathway, either by activating or inhibiting their function.5. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A. The designed protein sequence is ",
    "response#1": "MNIVLGVSGSVAAYRAADLARELMRAGHSVRVCLTDSAQKFVTAALFEALTGQACLQDTFEEPDRGRMAHIDWARQADIVVVAPATANTIAKIAHGIGDDMLTTLVLATTAPLVVAPAMNPQMYASEATREAMTKLRERATWVVEPTDGDVACGEHGQGKLAAISQIVEAVLEAAGTVLAGRVLLAVTGSIAAYKAPELVRRLREAGAEVRVVLSEGGAHFVTPMALQAVSGAPVRTALLDPAEESAMDHIALARWADALLVAPASADTLARLVQGRADDLLGAVHLATTAPVLAAPAMNHRMWAHPATRRNAEQLEADGVTLLGPAAGDQACGEEGPGRMLEPAELVALLGAPTAARPLEGRKVLITSGPTQEPIDDVRFVSNRSSGKMGAALARAALLMGAEVTVVSGPARAALPLQANIVHVRTAQEMLEAGLAHCADANLIIGAAAVADYRPAVRHRGKIRRSAEPTTLELHPNPDIIAEIAKAAPDAITVGFAAEPSRSLDAARDKLARKGLYAIAANDVSRDGIGFDADQNSLRLLWPGGGRELAPGTKAEVAHAILEIVAELV",
    "response#2": "MNIVLGVSGSVAAYRAADLARELMRAGHSVRVCLTDSAQKFVTAALFEALTGQACLQDTFEEPDRGRMAHIDWARQADIVVVAPATANTIAKIAHGIGDDMLTTLVLATTAPLVVAPAMNPQMYASEATREAMTKLRERATWVVEPTDGDVACGEHGQGKLAAISQIVEAVLPLLDGPERVLLEGRHIVVSAGGTREAIDPVRFIGNRSSGRMGAAIAAAALDRGARVTVVAANLEVPLPPGATVVTVESTAELRAALLGLTLAPDGRAGFDALIMAAAVADFRPADATPGKIERGSGLTLVLEPTPDVLTQVARLVRGTDSSGATTTRQAEPRPFLVGFAAEIGSFERVADKLRRKGVDLLIANDVAEPGSGFGSETNRVSILFADGTREDLPSSSKREVADAILDLVAERLT",
    "response#3": "MNIVLGVSGSVAAYRAADLARELMRAGHSVRVCLTDSAQKFVTAALFEALTGQACLQDTFEEPDRGRMAHIDWARQADIVVVAPATANTIAKIAHGIGDDMLTTLVLATTAPLVVAPAMNPQMYASEATREAMTKLRERATWVVEPTDGDVACGEHGQGKLAAISQIVEAVLGVLDDLVTTLEGRKVLITSGPTQEPIDDVRFVSNRSSGKMGAALARAALLMGAEVTVVSGPARAALPLQANIVHVRTAQEMLEAGLAHCADANLIIGAAAVADYRPAVRHRGKIRRSAEPTTLELHPNPDIIAEIAKAAPDAITVGFAAEPSRSLDAARDKLARKGLYAIAANDVSRDGIGFDADQNSLRLIAADGTEVWPEMSKADVAGQLAARIAAAL"
  },
  {
    "reference": "MTRIAINGFGRIGRNVLRALLERDSDLDVVAVNDLTEPATLARLLAYDTTSGRLGRPVTVEGNVLVVDGRRITVTAEREPANLPWAELGVDIVLEATGRFTSAKAARAHLDAGAKKVLVSAPADGADITLAFGVNTDAYDPDLHTIVSNASCTTNALAPLAKVLDDLAGIEHGFMTTVHAYTQEQNLQDGPHRDPRRARAAAVNIVPTTTGAAKAIGLVLPNLDGKLSGDSIRVPVPVGSIVELNTTVARDVTRDEVLDAYRAAAQGPLAGVLEYSEDPLVSSDITGNPASSIFDSELTRVDGRHIKVVAWYDNEWGFSNRVIDTLQLLAAR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.2. We require a protein that can specifically bind to the metabolites produced in the Carbohydrate degradation pathway.3. The protein design must be able to perform NAD binding in order to contribute to glycolytic process. The designed protein sequence is ",
    "response#1": "LLVVGGAPGYSGAARLTAAAALRAGAGRVTLVTHPDHAALANLDRPELMVRPAQTARDIDALLAGADAVAVGPGFGQEAWARGLWPAVADWRGPLVVDADALNLLAQAPRRRDDWILTPHPGEASRLLGSKAATVNADRLAALRELQDRYGGTVLLKGAGTLVAPGPQGTPACITGGHPGMATAGMGDVLTGIIGALRAQGLDAGAAAVTAAAWHIAAACTAAQRLGGQRGLLAGDVIEAV",
    "response#2": "LAPLAKVVNDNFGLVEGLMTTVHSYTATQKTVDGPSSKDWRGGRTAAQNIIPSSTGAAKAVGKVIPSLNGKLTGMSMRVPTSNVSVVDLTCRTDKSVTYDQIKEAMKKASANELKGIMSYSEDALVSSDLNGDPHSCIFDATAGIALNDHFIKLVAWRELDRRAMALPGMGDGKLMERAGAALLEALSEGFPSARTLGVLCGPGNNGGDGFVLARLAADAGWTVTVHAGEPGGQPDSDGARARRAWIDSGGEIRPLDAFSPDRSEVWADCLFGIGLGRPIEAPFAPLIETLNRSGAPVLAADVPSGVLIDTGSVRGVAVRARLTVTMIADKFGLRTGAAVDQAGRVRVAALNLE",
    "response#3": "MQKLDRVMMDDFGLLPEVLMERAGLGVAQVIIENFPPKDYQRVLILCGPGNNGGDGFVCARYLSEMGYQVFISIFSEKKKYKGEAKRNLTLAQRLGLPIKKINNLKNLKEIFETFRSELVVDALFGTGLKRAISGVFAEAIEFINTLSSKNQIKVVAIDLPSGISADTGEVLGVALKAHLTATFECFKHGLLNYPGKAYAGKVFVLPIGYAWTYLC"
  },
  {
    "reference": "MIFENTLAFAQQQDRNDPLAHYSDQFHHPVIDGKEVLYFTGNSLGLQPKTAKEHINQELEDWAKWGVEGHFHAVNPWVSYHEILTPASAELVGANESEVVCMNSLTTNLHLLFVSFYKPTAKRFKIISEAKMFPSDRYLLETQVRHHGLDPDDAIIEISPREGEYLIREEDIIAAVNDNADELALLFFGGVNYFTGQLFDMQRLTKAAHGVGALAGFDLAHAVGNVPMHLHDWDVDFAAWCTYKYLNSSAGNVGGIFVNDRHGNNTKINRFGGWWGHNKERRFLMENSFEPMTGAEGWQISNAPVMGMAILKSSLDIFHEAGIENLRAKSLKLTAYLEFVFNDIVNQFTDIKLEIITPTDPTQRGCQLSIKLVGTNKEFFEALTKAGVIADFREPDVIRLAPTPLYNSFEDVYLLGQTLKVLAQNWRQHG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that is capable of catalyzing reactions within the NAD(+) biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "EGFATLTGAEPSEVVAMNTLTVNLHLLMASFYRPTSSRYKIVIESNAFPSDRYAAASQIRMHGFDPDDGLLEWTPRDEDGYLDIADLESLLEKEGDSTALLLLPGVQYYTGQVLDMARICRIGRDAGCSVGLDLAHAIGNVDLALHKWAPDFAAWCTYKYLNAGPGAIH",
    "response#2": "EGFATLTGAEPSEVVAMNTLTVNLHLLMASFYRPTSSRYKIVIESNAFPSDRYAAASQIRMHGFDPDDGLLEWTPRDEDGYLDIADLESLLEKEGDSTALLLLPGVQYYTGQVLDMARICRIGRDAGCSVGLDLAHAIGNVDLALHKWAPDFAAWCTYKYLNAGPGAIL",
    "response#3": "EGFATLTGAEPSEVVAMNTLTVNLHLLMASFYRPTSSRYKIVIESNAFPSDRYAAASQIRMHGFDPDDGLLEWTPRDEDGYLDIADLESLLEKEGDSTALLLLPGVQYYTGQVLDMARICRIGRDAGCSVGLDLAHAIGNVDLALHKWAPDFAAWCTYKYLNAGPGAIL"
  },
  {
    "reference": "MRCPFCGAEDTSVVDSRISEEGARIRRRRRCVECEKRFTTYETVELRFPQVIKQDGNRVEFNREKLYTSFARALHKRPVPTGQVDAAIERILQKLLGSGVQEISSRTIGEWVMQELYSLDKVAYIRFASVYRSFEDVGDFQEVIREVQSSPQNGDGPSS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit ATP binding.2. The protein must exhibit the following characteristics: Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR- boxes.. The designed protein sequence is ",
    "response#1": "SVLKKDGGRELFDKQKLFTGISRACEKTNFSSEEIINFVDGIESQIVQDSNKDIKSSHIGELILKNLRKENEVAYIRYASVYRKFS",
    "response#2": "SVLKKDGGRELFDKQKLFTGISRACEKTNFSSEEIINFVDGIESQIVQDSNKDIKSSHIGELILKNLRKENEVAYIRYASVYRKFC",
    "response#3": "SVLKKDGGRELFDKQKLFTGISRACEKTNFSSEEIINFVDGIESQIVQDSNKDIKSSHIGELILKNLRKENEVAYIRYASVYRKFS"
  },
  {
    "reference": "MEYMAESTDRSPGHILCCECGVPISPNPANICVACLRSKVDISQGIPKQVSISFCKQCQRYFQPPGTWIQCALESRELLALCLKKIKAPLSKVRLVDAGFVWTEPHSKRLKVKLTIQKEVMNGAILQQVFVVDYVVQSQMCGDCHRVEAKDFWKAVIQVRQKTLHKKTFYYLEQLILKYGMHQNTLRIKEIHDGLDFYYSSKQHAQKMVEFLQCTVPCRYKASQRLISQDIHSNTYNYKSTFSVEIVPICKDNVVCLSPKLAQSLGNMNQICVCIRVTSAIHLIDPNTLQVADIDGSTFWSHPFNSLCHPKQLEEFIVMECSIVQDIKRAAGAGMISKKHTLGEVWVQKTSEMNTDKQYFCRTHLGHLLNPGDLVLGFDLANCNLNDEHVNKMNSDRVPDVVLIKKSYDRTKRQRRRNWKLKELARERENMDTDDERQYQDFLEDLEEDEAIRKNVNIYRDSAIPVESDTDDEGAPRISLAEMLEDLHISQDATGEEGASMLT",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein with RNA binding functions is required to enable positive regulation of protein localization to nucleolus, protein transport, positive regulation of protein binding.2. The protein's functional design necessitates the inclusion of a Nuclear and nucleolar localization signal motif. The designed protein sequence is ",
    "response#1": "SDQLKPVFVGEFPQLLRDEQSYPGDACVSGGMDKETCLSWFITGSKVFVWSHLTTLPSRKCVVLELPVVVLVNEESGSGLQDGKSWLVNVVSWDTSAGAATRASRSRSPVGVVMCNRKTRAVVYWSDIFSGQEAAPAEKARHLIKRQSNGIRSSRAENSDLNSLITTAVAAAERLCIAIACSSNGELWQFTCSPTGVKSNQVQLNISSSSVSEGYPRSLIWRFSQGLARESCWEFLMLTDCDIHCFTIEPYPDLTVSEVWQHEIVGTDGDSGIKKDIASQKQIWPLDLQVDDQGKVITVLVATICMDRASSSSYTQYSLLTLQHKSEMRFADGREEKVLEKQGPIQVIIPKARVEDKDFLFSMRLRVGGRPPGSAIILSGDGTATVCYCHGSSTRLYKFDLPYDAGKVLDASVLSSTDEHEYGAWTVLTEKAGVV",
    "response#2": "SDQLKPVFVGEFPQLLRDEQSYPGDACVSGGMDKETCLSWFITGSKVFVWSHLTTLPSRKCVVLELPVVVLVNEESGSGLQDGKSWLVNVVSWDTSAGAATRASRSRSPVGVVMCNRKTRAVVYWSDIFSGQEAAPAEKARHLIKRQSNGIRSSRAENSDLNSLITTAVAAAERLCIAIACSSNGELWQFTCSPTGVKSNQVQLNISSSSVSEGYPRSLIWRFSQGLARESCWEFLMLTDCDIHCFTIEPYPDLTVSEVWQHEIVGTDGDSGIKKDIASQKQIWPLDLQVDDQGKVITVLVATICMDRASSSSYTQYSLLTLQHKSEMRFADGREEKVLEKQGPIQVIIPKARVEDKDFLFSMRLRVGGRPPGSAIILSGDGTATVCYCHGSSTRLYKFDLPYDAGKVLDASVLSSTDEHEYGAWTVLTEKAGVQ",
    "response#3": "SDQLKPVFVGEFPQLLRDEQSYPGDACVSGGMDKETCLSWFITGSKVFVWSHLTTLPSRKCVVLELPVVVLVNEESGSGLQDGKSWLVNVVSWDTSAGAATRASRSRSPVGVVMCNRKTRAVVYWSDIFSGQEAAPAEKARHLIKRQSNGIRSSRAENSDLNSLITTAVAAAERLCIAIACSSNGELWQFTCSPTGVKSNQVQLNISSSSVSEGYPRSLIWRFSQGLARESCWEFLMLTDCDIHCFTIEPYPDLTVSEVWQHEIVGTDGDSGIKKDIASQKQIWPLDLQVDDQGKVITVLVATICMDRASSSSYTQYSLLTLQHKSEMRFADGREEKVLEKQGPIQVIIPKARVEDKDFLFSMRLRVGGRPPGSAIILSGDGTATVCYCHGSSTRLYKFDLPYDAGKVLDASVLSSTDEHEYGAWTVLTEKAGVG"
  },
  {
    "reference": "MEEVVIAGMSGKLPESENMQEFWDNLINGVDMVTDDDRRWKTGLYGLPRRAGKLKDLSKFDASFFGVHPKQAHTMDPQLRLLLEVTYEAIVDGGINPASLRGTSTGVWVGVSGSEASEALSRDPETLLGYSMVGCQRAMMANRLSFFFDFKGPSVALDTACSSSLLALHSAFQAIRSGECPAAIVGGINVMLKPNTSLQFLRLGMLSPEGACKAFDKAGNGYCRAEAVVAVLLTKKSLAKRVYATILNTGTNTDGYKEQGVTFPSGEAQAYLLRSMYEPAGVDPESLEYIEAHGTGTTVGDPQELNCIAQALCATRQSPLLIGSTKSNMGHPEPASGLAALAKVLLSLEHGQWAPNLHFHNPNPKIPALQDGRLRVVDQPLPVGYGNVSINSFGFGGSNVHVILQPNAVRPPAPTPPALPRLLQASGRTPEAVQGLLEQGRRHSQDLAFLNMLNDIAATPTSSMPFRGYTVLGIEGEAQEVQQVPAGDRPLWFICSGMGTQWLGMGLSLMRLSNFRESILRSDEAVSQFGLKVSDLLLSTDENTFDDIVHAFVSLTAIQIALIDLLTAMGLRPDGIVGHSLGEVTCGYADGCLTQEEAVLAAYWRGQCIKEADMPPGAMAAVGLSWAECKKRCPPGIVPACHNSEDTVTISGPQAAMSEFVEQLKQEGVFAKEVRTGGMAFHSYFMESIAPTLLQALKKVIRQPRPRSPRWLSTSVPEAEWQGSLARTSSAEYNVNNLVSPVLFQEALWHVPENAVVVEVAPHALLQA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein have Carrier domains with specific sequence motifs that are crucial for its activity.2. A protein with palmitoyl-[acyl-carrier-protein] hydrolase activity, 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity, [acyl-carrier-protein] S-malonyltransferase activity is needed to enhance fatty acid biosynthetic process in a specific context.3. The protein must exhibit the following characteristics: Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH.4. The protein design should take into account the physiological conditions of the fatty acid biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MEDIVIAGISGRLPESDSLEEFWQNLFNGVDMVTEDDRRWKPGLYGLPRRNGKLKEIDRFDAAFFGVHPKQAHTMDPQLRLMLEISYEAIVDGGINPVSMRGTKTGVYIGVSGSEAGEAFSKDPEELLGYSMTGCQRAMFANRLSYFFDFNGPSTAIDTACSSSLLALENAFQAIRHGQIDSALVGGVNLLLKPNTSVQFMKLGMLSPDGSCKSFDSSGNGYCRSEAAVAVLLMKKSMAKRVYATVLNAGNNTDGYKEQGVTFPSGDMQQLLVRSLYQEANISPEQVEYIEAHGTGTKVGDPQEVNGIVSVFCESQRDPLLIGSTKSNMGHPEPASGLAALAKVVLSLEHGVWAPNIHFQNPNPDIPALTDGRLRVVSEPIPVRGGIVGINSFGFGGSNVHVILSPNAKPVPVPPVPPNNKIEALKNKTMKVVTEPTKLDGPIIATTTLGMPSSIGHVLLKQNPKTKEYSQLGPSQRLILLSSRTEKGISEAIDRVTSLPRDDEYLGLIQNVFKDNIVGHFHRGYAFSS",
    "response#2": "MDEIVIAGMSGRLPESNNLEEFWENLINGVDMVKEDNRRWKPGLYGLPRRNGKLKDISHFDATFFGVHPKQANTMDPQLRLMMEIAYEAIVDGGLNPASMRGSKTGVYIGVSGSEAGEAFSRDPEELLGYSMTGCQHAMLANRLSYCFDFTGPSTSIDTACSSSLLALENAFHAIRHGQCDAALVGGVNLLLKPNTSVQFMKLGMLSPEGTCKSFDSSGNGYCRSEAAVAVLLTKRSFAKRVYATVVNAGSNTDGFKEQGITFPSGEMQQRLVHSLYQEVNIPPEQVEYVEAHGTGTKVGDPQEVNGIVSVFCQSKRQPLLIGSTKSNMGHPEPASGLAALAKVLLSLERGVWAPNLHFNSPNPDIPALTDGRIQVVDRPIPIRGGIVGINSFGFGGSNVHVILRPAGKPAPSAPSAPPNNKIEALKNKTMKVVTEPTKLDGPIIATTTLGMPSSIGHVLLKQNPKTKEYSQLGPSQRLILLSSRTEKGISEAIDRVTSLPRDDEYLGLIQNVFKDNIVGHFHRGYAVYSGMGSQWGSMAKDLLQFDVFNKSIHRCAEILRPEGIDLLEILTRSTDENFADIVSSFVSITTMQVALTDLLSSLGIHPAGIVGHSVGELGCAYADGALSAEQAVLATYYRGRAITESNLPKGKMAAVGLAWDDAHKRVPSDCYPVCHNNQDNCTIAGPGDSIDALVAQLNSEGVFAKAVSSSGFAFHSKYIAGAAPKLRQMLEKIIPTAKNRSPRWVSSSIPESAWNTPVAKQSSAAY",
    "response#3": "MDDIVIAGISGRLPESDNLEEFWENLFNGVDMVTEDDRRWKPGLYGLPKRNGKLKDISRFDAAFFGVHPKQAHTMDPQLRLMLEISYEAIVDGGINPAALRGSKTGVYIGVSGSEAGEAFSRDPEELLGYSMTGCQRAMFANRLSYFFDFNGPSTAIDTACSSSLLALENAFQAIRHGHCEAALVGGVNLLLKPNTSVQFMKLGMLSPEGTCKSFDSSGNGYCRSEAAVAVLLTKRSMAKRVYATVVNAGNNTDGYKEQGVTFPSGEMQQRLVRSLYQEANITPEQVEYIEAHGTGTKVGDPQEVNGIVSVFCQSKRDPLLIGSTKSNMGHPEPASGLAALAKVVLSLERGVWAPNLHFNSPNTDIPALTDGRVRVVNKPIPVRGGIVGINSFGFGGSNVHVILRPNRKSPGPLPRTIPRPNNKIEALKNKTMKVVTEPTKLDGPIIATTTLGMPSSIGHVLLKQNPKTKEYSQLGPSQRLILLSSRTEKGISEAIDRVTSLPRDDEYLGLIQNVFKDNIVGHFHRGYAPNNKIEALKNKTMKVVTEPTKLDGPIIATTTLGMPSSIGHVLLKQNPKTKEYSQLGPSQRLILLSSRTEKGISEAIDRVTSLPRDDEYLGLIQNVFKDNIVGHFHRGYAYN"
  },
  {
    "reference": "MTQLAIGEATPHGATYDGHGVNFTLFSAHAERVELCVFDSRGNERRYDLPGRRGDVWHGYLAGARPGLRYGYRVHGPWQPAQGHRFNPAKLLLDPYARRVEGELKDHPLLHGGHDEPDYRDNAAVAPKSVIISDHYDWEDDAAPRTPWGKTVIYEAHVKGLTYLHPELPQEIRGTYKALGHPVMVAYFKQLGITALELLPVAQFASEPRLQRMGLTNYWGYNPMAMFALHPAWASSPETALDEFRDAVKALHRAGIEVILDIVLNHSAELDLDGPTFSLRGIDNRSYYWIRDDGDYHNWTGCGNTLNLSHPGVVEYACECLRYWVETCHVDGFRFDLASVMGRTPTFRQDAPLFAAIKACPVLSTVKLIAEPWDIGEGGYQVGNFPPPFAEWNDHFRDAARRFWLPRNLTTGEFACRFAASSDVFKRNGRTPGASVNLLTAHDGFTLRDCVCFNQKHNEANGEENRDGTNSNYSDNHGKEGLGGPLDLMERRRDSIHALLATLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDWQQANRGLTTFTAALIRLRQQIPALTGNSWWEEGDGNVRWLNKNAQPLSADEWQNGPKLMQILLSDRFLIAINATLEVTDIVLPEGEWRAVPPFAGEDNPVITAVWQGPAHGLCVFQRG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be designed to catalyze the following reaction Hydrolysis of (1->6)-alpha-D-glucosidic linkages to branches   with degrees of polymerization of three or four glucose residues in   limit dextrin.. The designed protein sequence is ",
    "response#1": "PVMVAYFKQLGITALELLPVAQFASEPRLQRMGLTNYWGYNPMAMFALHPAWASSPETALDEFRDAVKALHRAGIEVILDIVLNHSAELDLDGPTFSLRGIDNRSYYWIRDDGDYHNWTGCGNTLNLSHPGVVEYACECLRYWVETCHVDGFRFDLASVMGRTPTFRQDAPLFAAIKACPVLSTVKLIAEPWDIGEGGYQVGNFPPPFAEWNDHFRDAARRFWLPRNLTTGEFACRFAASSDVFKRNGRAPGASVNLLTAHDGFTLRDCVCFNQKHNEANGEENRDGTNSNYSDNHGKEGLGGPLDLMERRRDSIHALLATLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDWQQANRGLTTFTAALIRLEEGDGNVRWLNKNAQPLSADEWQNGPKLMQILLSDRFLIAINATLEVTDIVLPEGEWRAVPPFAGEDNPVITAVWQGPAHGLCVL",
    "response#2": "PVMVAYFKQLGITALELLPVAQFASEPRLQRMGLTNYWGYNPMAMFALHPAWASSPETALDEFRDAVKALHRAGIEVILDIVLNHSAELDLDGPTFSLRGIDNRSYYWIRDDGDYHNWTGCGNTLNLSHPGVVEYACECLRYWVETCHVDGFRFDLASVMGRTPTFRQDAPLFAAIKACPVLSTVKLIAEPWDIGEGGYQVGNFPPPFAEWNDHFRDAARRFWLPRNLTTGEFACRFAASSDVFKRNGRAPGASVNLLTAHDGFTLRDCVCFNQKHNEANGEENRDGTNSNYSDNHGKEGLGGPLDLMERRRDSIHALLATLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDWQQANRGLTTFTAALIRLEEGDGNVRWLNKNAQPLSADEWQNGPKLMQILLSDRFLIAINATLEVTDIVLPEGEWRAVPPFAGEDNPVITAVWQGPAHGLCVPHGATYDGHGVNFTLFSAHAERVELCVFDSRGNERRYDLPGRRGDVWHGYLAGARPGLRYGYRVHGPWQPAQGHRFNPAKLLLDPYARRVEGELKDHPLLHGGHDEPDYRDNAAVAPKSVVITV",
    "response#3": "VVMDDRYDWEDDAPPNIAWGETVIYEAHVRGLTRLHPDIPPALRGTYAALGHPAMIAWFQRLGITALELQPVAKFCSEPRLQRLGLANYWGYNPLALWAIETRYASQPAPQAALNEFRDAVKALHKAGIEVILDVVLNHSAELDLEGPTFSLRGIDNRSYYWIQDDGDYHNWTGCGNTLNLSHPAVVDYAIGCLKFWVEQCHVDGFRFDLATVMGRTPEFRQDAPLFEAMRRDRSLSAVKLIVEPWDIGPGGYQVGNYPPPFAEWNDRFRDDMRRFWLRSELDVGEVATRVAASADVYRRNGREPWACVNLVTAHDGFTLRDCVSFNGKHNEANGEDNRDGAWENHSNNHGYEGLGGGQSVQDARRASTHALLASLLLSQGTPMLLAGDEQGHSQHGNNNAYCQDNELTWFDWSQADEGLVAYTAALIRLRQRLPALRG"
  },
  {
    "reference": "MPQLLRLATAGGVDDGKSTLIGRLLHDSKTIFEDQLAAVERTSRERGDDYPDLALLTDGLCAEREQGITIDVAHRYFATPKRKFIIADTPGHVQYTRNMVTGASNADLALVLVDARKGVLEQSRRHAFLASLLGIPHLVLCVNKMDLVGWSAERFSEIREEFSQFAMKLNVADLTFVPVSALHGDNIVHRSASMPWYEGTSLLHHLEEVHISSDRNLIDARFPVQYVIRSQQHDFRGYAGTVAGGVFKPGDEIVVLPSGFTSSVRAIWGPGGEPIDEAFAPQAVTVQLADELDVSRGDLICRPNNRPHLGTDVDAMICWFAEHAALRPGARYTIRHTTRTTKARVSALDYRLDVRTLHRDETTETLSLNDIGRIRLRTQQPLLFDPYRRNRGTGSFILIDETTNDTVAAGMITGPVLPAAQVVWQSTAVGRHERATRGLTVWLTGLSASGKSAVAVELERRLVAAGRPAYLLDGDNVRHGLNADLGFSAPDRTENVRRLGEVARLFADAGVVAVVSLISPYRADRELVRATHQAAGLPFVEVFVDTPVEVCEARDPKGMYARARAGEITGFTGIDDPYEAPDTPDLVLRPADGDPAAMAAKVLALLEG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have a high affinity for ATP.2. A protein with sulfate adenylyltransferase (ATP) activity, ATP binding, GTPase activity, adenylylsulfate kinase activity functions is required to enable hydrogen sulfide biosynthetic process, phosphorylation.3. The Phosphoserine intermediate of the protein must be engineered to enable effective catalysis of adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate   + ADP + H(+).4. A protein should be able to manipulate the activity of enzymes in the Sulfur metabolism, hydrogen sulfide biosynthesis, sulfite from sulfate: step 2/3 pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "MLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDGRKGILPQTKRHSYLAHLIGIRHIVLAVNKMDLVGYDRDIFDNIVRDYREFASEIGITDFTAIPISGFKGDNIPAITTAIKERLLTKDQLRFITCGSVDDGKSTLIGRLLHDSKIIYEDQLTALAHDSARHGTNGNDVDFALLVDGLEAEREQGITIDVAHRFFTTPRRSFMVLDTPGHEQYTRNMATGASNAQLAIILVDARKGVLVQTKRHSFICSLLGIRNIVLAVNKIDLIKYKKEIFDRIVEDYIAFSASLRFTSIVPIPISARYGDNVASRSGNFEWYDGPCVLDYLESIDIQSNAVIQPMRFPVQYVNRTRSDFRGFCGTLVSGRIAPGDEVVVLPSGRRSRVAEIIGNTGAVHRAQVGNTGAVKSTEVEYAGTMDQARAGQAITLTLADEIDISRGDVLVHPQNRPVAEVSDQFGVHLLWMDAEPLKPGRNYWIQTGTSVVGGRVSEIKHQIDVNTQEELAARELSLNAVARVDLELDRAVPFEPYALNRDLGSFVIIDRQTNGTVGAGVINHSLRRQKNITWQKHSITKEERARIKGQKPVVLWFTGLSGSGKSTIANIVEKKLHALGRHTYLLDGDNVRHGLNRDLGFTEEDRVENIRRVAEVAKLMVDAGLIVLVSFISPFRSERRMARDLFEEGE",
    "response#2": "MLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDGRKGILPQTKRHSYLAHLIGIRHIVLAVNKMDLVGYDRDIFDNIVRDYREFASEIGITDFTAIPISGFKGDNIIDFIDYYKNLIDLLRFITCGSVDDGKSTLIGRLLFDSKMIFEDQLAALESDSKKVGTTGDDMDLALLLDGLEAEREQGITIDVAYRFFATDKRRFIVADTPGHEQYTRNMATGASTADAAVILLDARKGILTQTRRHSFICSLLGVRNIVLAVNKMDLVDYSREVFEKIREDYEAFAKTVGYENVTCIPLSALKGDNVKSFLRLITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEVDSRKVGTTGDKIDLALLVDGLQAEREQGITIDVAYRFFSTDKRKFIIADTPGHEQYTRNMATGASTGELAIILIDGRKGVLTQTRRHSYIVSLLGIRHVVLAINKMDLVDYDRKTFDNIKRDYLEFAKALNFSDITAIPMSALEGDNVLRKTQAMPWYQGPSLMQHLESVDVSRELSDQFEVKIVWMSDEALMPGRPYLFKGAANTASMVVTRLKHSINVNTLEQMATKRLMLNEIGVANISLDRQITFDPYKDNRETGSFIIIDKLTNNTVGAGMRVSELFDARVVWMNDRPLLPGRQYNLKLASSEVPATPEAIHHRIDVNTLEHHPADQLGLNEIGYCRIRASRALAFDAYDSNPATGAFILVDRVSNLTVGAGM",
    "response#3": "MLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDGRKGILPQTKRHSYLAHLIGIRHIVLAVNKMDLVGYDRDIFDNIVRDYREFASEIGITDFTAIPISGFKGDNILVGVGDVRDWLKELLRFITCGSVDDGKSTLIGRLLFDTKQVFEDQLAAVGKDSRKYGTTGETPDLALLVDGLQAEREQGITIDVAYRYFTTEKRKFIIADTPGHEQYTRNMVTGASTADLAIVLIDARHGLQVQTRRHSYLVHMLGIRHVVVAINKMDAVDYDRGVFDRIRGDYEAFANQLGIAHFECVPVCALNGANVTRDAEEMDWYDGPTVLDLLESTPVHEHDLFRLPVQWVCRPNQNFRGFAGPLLGGPLKVGDAVVALPSGRRSTVAGLHVGSETVDQALAGQSVIVELTEEIDVSRGDVLARPDRVADCLDARVVWMHERPMLPGRQYQMKLACSELRVTPETLHHRIDVNTLEHRPAEQLELNEIGYCRLRLGRMLAFDAYEANPGMGSFILIDTVSNLTVGAGMRVSELFDARVVWMNDRPLLPGRQYNLKLASSEVPATPEAIHHRIDVNTLEHHPADQLGLNEIGYCRIRASRALAFDAYDSNPATGAFILVDRVSNLTVGAGMFDARVVWMNDRPLLPGRQYNLKLASSEVPATPEAIHHRIDVNTLEHHPADQLGLNEIGYCRIRASRALAFDAYDSNPATGAFILVDRVSNLTVGAGM"
  },
  {
    "reference": "MAKRQLLMLGIRTSFHTIAAVLVAGLIFTAVFLSRNSLPKENPQSHGVTDRGGDSGRECNLFEGKWVFDNVSYPLYKEEDCKFMSDQLACEKFGRKDLSYKFWRWQPHTCDLPRFNGTKLLERLRNKRMVYVGDSLNRGQWVSMVCMVSSVITNPKAMYMHNNGSNLITFKALEYNATIDYYWAPLLVESNSDDPTNHRFPDRIVRIQSIEKHARHWTNSDIIVFNSYLWWRMPHIKSLWGSFEKLDGIYKEVEMVRVYEMALQTLSQWLEVHVNPNITKLFFMSMSPTHERAEEWGGILNQNCYGEASLIDKEGYTGRGSDPKMMRVLENVLDGLKNRGLNMQMINITQLSEYRKEGHPSIYRKQWGTVKENEISNPSSNADCIHWCLPGVPDVWNELLYAYILDHHSS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that can perform xylan O-acetyltransferase activity in Golgi apparatus, Golgi membrane to support xylan biosynthetic process.2. The designed protein should contain at least one GDS motif motif to achieve its intended function.3. For general function, the protein need meet that Probably involved in maintaining esterification of pectins ; May act as a bridging protein that binds pectin and other cell wall polysaccharides4. The designed protein should have at least Helical; Signal-anchor for type II membrane protein transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "LPSFNATLMLEMLRGKRMMFVGDSLNRGQYVSLVCLLHRSIPESSKSMETFDSLTVFRAKNYNATIEFYWAPFLAESNSDDAVVHRIADRIVRGTALEKHARFWKGADILVFNSYLWWMTGQKMKILQGSFEDKSKDIVEMETEEAYGMVLNAVVRWVENNMNPRNSRVFFVTMSPTHTRSKDWGDDSDGNCYNQTTPIRDLSYWGPGTSKGLMRVIGEVFSTSKVPVGIVNITQLSEYRKDAHTQIYKKQWNPLTPEQIANPKSYADCTHWCLPGLQDTWNELLYA",
    "response#2": "LPRFNASDLLERSRNGRIVFVGDSIGRNQWESLMCMLSQAIPNKSEIYEVNGNPITKHKGFLSMRFPRENLTVEYHRSPFLVVIGRPPDKSPKEIKTTVRVDEFNWQSKRWVGSDVLVFNSGHWWNEDKTVLTGCYFEEGRKVNKTMGVMEAFGKSLKTWKSWVLEKLDPDKSYVFFRSYSPVHYRNGTWNTGGLCDAEIEPETDKRKLEPDASHNEYIYKVIEEMRYRHSKVKFLNITYLTEFRKDGHISRYREQGTSVDVPQDCSHWCLPGVPDTWNEILYCS",
    "response#3": "LPRFNASDLLERSRNGRIVFVGDSIGRNQWESLMCMLSQAIPNKSEIYEVNGNPITKHKGFLSMRFPRENLTVEYHRSPFLVVIGRPPDKSPKEIKTTVRVDEFNWQSKRWVGSDVLVFNSGHWWNEDKTVLTGCYFEEGRKVNKTMGVMEAFGKSLKTWKSWVLEKLDPDKSYVFFRSYSPVHYRNGTWNTGGLCDAEIEPETDKRKLEPDASHNEYIYKVIEEMRYRHSKVKFLNITYLTEFRKDGHISRYREQGTSVDVPQDCSHWCLPGVPDTWNEILYCG"
  },
  {
    "reference": "METETGKGATAPPAESSGVEQDTAAVGAPADQPPKTNANATSNANGEDQPANGQKANPKDPSRPRRKKARRACFACQRAHLTCGDERPCQRCIKRGLQDACHDGVRKKAKYLHDAPDGALMPGIGGNNFYNNNSMSNGVPSGGINMNGANTVNSAASTQNSSANFYPTPQSNYSLYQENPINHQNSFPSQSPVSPTFSLKTNPTPRNTAPNNNNNNALTSSMPQPATTGVSNAPNQSQNPFAGPFFDPSDPALFNFDLSSMNFENRYGALEFGMLGHMATGAGDSPDSGTHRGSMGRSGSTQFASTPIGGTTTFGESPQNQQPFMFGDPLLNEWPSGQTSGQPHVNVGVYPQSSQGNVIPGHLSKPDAPHAFAIESGPNNFTSPGAATSPQINSGGYEDANAFNNVVTKSNGLSVNGQQRPPTISTPSLKHQSLQMNKRRHRNPSAVYESVKEPYAYTSRFHSLTAFIQRRFSPQKTLQIAKALASIRPSFIATTKTLNRDDLIFMEKCFQRTLWEYEDFINACGTPTIVCRRTGEVAAVGKEFSILTGWKKEVLLGKEPNYNVNTGGSSAANSRNITPRSSVESTGRPHPVFLAELLDDDSVVEFYEDFARLAFGDSRGSVTTRCKLLKYKTKEDMEAAQSDDNGQRWNNHLRKGGIANEAGMNQLGFKDGKVECAYCWTVKRDVFDIPMLIVMNVRLPLP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Activator of gluconeogenetic genes like acuF, acuG and acuN.; Transcription factor which regulates nonfermentable carbon utilization2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.3. The designed protein should be involved in gluconeogenesis, cellular response to starvation by localizing to nucleus and exhibiting DNA-binding transcription factor activity, zinc ion binding, RNA polymerase II transcription regulatory region sequence-specific DNA binding. The designed protein sequence is ",
    "response#1": "TPTIVCRRTGEIAAVGKEFSILTGWRKEVLLGKEPNHNVNTGGSSGLLTGSTSRGSYTPRPYSSDQFNSSTTATPRTQPVFLAELLDDDSVIEFYEDFAKLAFGDSRGSVMTTCKLLKYKTKADSGVIASGNGEVGAAQNNEVGSGEANELNSSSNGTTSTGRGQRRWGKGEIAGEAGMNQLGFRDGKVECSYCWTVKRDVFDIPMLIVMN",
    "response#2": "TPTIVCRRTGEIAAVGKEFSILTGWRKEVLLGKEPNHNVNTGGSSGLLTGSTSRGSYTPRPYSSDQFNSSTTATPRTQPVFLAELLDDDSVIEFYEDFAKLAFGDSRGSVMTTCKLLKYKTKADSGVIASGNGEVGAAQNNEVGSGEANELNSSSNGTTSTGRGQRRWGKGEIAGEAGMNQLGFRDGKVECSYCWTVKRDVFDIPMLIVMN",
    "response#3": "IGTPTCIWRRNGQISYVNEEFEILCGWTREELLNKMTFIVEIMDDESVRDYFKTLSKVAYRDFRGSEKMKVCRLLSPIKGKIIHCCCMWTLKRDVSGLPLMILGNFMPK"
  },
  {
    "reference": "MKSNYAVIVAAGKGKRMKMPINKQFICIQGKPILYYSISVFSKNPLVDKIVLVCAEDEIEYCKQEIVKKYNFDKVVKIVSGGKERQHSVFNALKVLENCSVVLIHDGARPFVTDRIIEDGIKYSNMYGACACGVVPKDTIKIKGKEGFSYKTLVRKELFIVQTPQCFDYNLIYDCHKKLANNKVQVTDDTTVAEYFGNMVYLYEGSYDNIKITTPEDLIIAENIFKTHKYI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein should be able to manipulate the activity of enzymes in the isopentenyl diphosphate biosynthesis via DXP pathway, isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 2/6, Isoprenoid biosynthesis pathway, either by activating or inhibiting their function.2. The protein have enhanced 2-C-methyl-D-erythritol 4-phosphate + CTP + H(+) = 4-CDP-2-C-   methyl-D-erythritol + diphosphate.3. A protein with 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity functions is required to enable isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway, terpenoid biosynthetic process. The designed protein sequence is ",
    "response#1": "MLIGFGYDVHRLVPGRPLVLGGVEIPSKRGLEGHSDADVALHALMDALLGAAGLGDIGTHFPPTDPAWAGANSLDLLRRVVGLLAEEGYRPVNCDITVVAERPKIGPYVPAMRERIGAVLGVEPRRVSIKATTNEQMGFVGREEGIAAYAVALAERA",
    "response#2": "MLIGFGYDVHRLVPGRPLVLGGVEIPSKRGLEGHSDADVALHALMDALLGAAGLGDIGTHFPPTDPAWAGANSLDLLRRVVGLLAEEGYRPVNCDITVVAERPKIGPYVPAMRERIGAVLGVEPRRVSIKATTNEQMGFVGREEGIAAYAVALIAAT",
    "response#3": "MLIGFGYDVHRLVPGRPLVLGGVEIPSKRGLEGHSDADVALHALMDALLGAAGLGDIGTHFPPTDPAWAGANSLDLLRRVVGLLAEEGYRPVNCDITVVAERPKIGPYVPAMRERIGAVLGVEPRRVSIKATTNEQMGFVGREEGIAAYAVALIAAE"
  },
  {
    "reference": "MKHNHKLALVLAAGKGTRMRSDLPKVLQSLGGKAMIKHLLETLQQVKDIDIAIIYGYQGNVLQEALNEDYQNLSWVAQEEQLGTGHAVRQALPLLETNGVTLIVFGDGPFLQLDTINKLINVAEEKGAALLTATVDNPFGYGRIIRNEQKEIVGIIEEKDTNEMEKGIKEINVGVMAIQNHLLAKFIPMISCQNAQKEYYLTDIIGLLSANGETVDSVSIKEVDEMIGINDKIQLAEAERIYRQQQVKKLLDKGVTIIDPLRCDIHGEVEVGKDVIIEPNVFFKGKVIIGDRVIIEAGSILENCTIGNDSVIHAYSIIEKTTVEQAVNVGPFARLRPNTILHSHSRIGNFVEIKGSTIGIESKVNHLSYIGDSVVGSKVNIGAGTITCNYDGKNKFKTMIGDNVFVGSNTALVAPLSLGNNVTIGAGSVITKDIIPNALALTRAKIKIKEDWKKFESN",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Ideal protein should have a binding affinity for key enzymes in the UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway to enhance the overall efficiency of the pathway.2. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.3. The protein design must be able to perform magnesium ion binding, glucosamine-1-phosphate N-acetyltransferase activity in order to contribute to UDP-N-acetylglucosamine biosynthetic process. The designed protein sequence is ",
    "response#1": "DGDIMLGILAKYLKEKGLLKGDKIVGTIMTNLGLEEYLKNINVELIRTKVGDRYVLDEILKYGLNLGGETSGHIILFDYMSTGDGLLTSLFLLKILKEKGVKLSELAKR",
    "response#2": "DGDIMLGILAKYLKEKGLLKGDKIVGTIMTNLGLEEYLKNINVELIRTKVGDRYVLDEILKYGLNLGGETSGHIILFDYMSTGDGLLTSLFLLKILKEKGVKLSELAKE",
    "response#3": "DGDIMLGILAKYLKEKGLLKGDKIVGTIMTNLGLEEYLKNINVELIRTKVGDRYVLDEILKYGLNLGGETSGHIILFDYMSTGDGLLTSLFLLKILKEKGVKLSELAKA"
  },
  {
    "reference": "MGGKWSKRSIEGWPQVRERIRQTPPGAAAEGVGAVSKDLEKHGAVTSSNMNNAECVWLEAQEEEEVGFPVRPQVPLRPMTFKAAFDLGFFLKEKGGLDGLIYSKKRKEIFDLWVYHTQGFFPDWQNYTPGPGIRYPLCFGWCYKLVPVDPREVEEETKGEDICLLHPLSQHGIDDEEGEVLMWKFDSSLARRHVARELHPEYYKDC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1.2. The designed protein must be able to perform SH3 domain binding under specific conditions. The designed protein sequence is ",
    "response#1": "MPQLDTSTWFTILFTW",
    "response#2": "MPSSD",
    "response#3": "SCHFG"
  },
  {
    "reference": "MVTVTVDKNENLEKALKRFKRMIEKEAIIREWKRREYYEKPSTIRVKKEKAFKRKQAKKVRKLKQKTNR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that contributes to translation by functioning in ribosome, ribonucleoprotein complex with structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "LESRLDNLVYRLGLARTRRQARQLAV",
    "response#2": "SRYTGPMWKISRRLGISLSGTGKELQKRPYPPGQHGPGQRRKLSEYGLQLQEKQKLRHMYGVNERQFRKTFEEAGKMPGKHGENFMILLG",
    "response#3": "LESRLDNLVYRLGLARTRRQARQLGV"
  },
  {
    "reference": "MRVLGLETSCDETGVALYDSERGLLADALFSQIDLHRVYGGVVPELASRDHVKRMLPLIRQVLDESGCKPADIDAIAYTAGPGLVGALLVGASCAQAMAFAWGVPAVGVHHMEGHLLAPMLEEQPPRFPFVALLVSGGHTQLVRVDGIGRYQLLGESVDDAAGEAFDKTAKLIGLGYPGGPEIARLAERGTPGRFVFPRPMTDRPGLDFSFSGLKTFSLNTWQRCVEAGDDSEQTRCDIALAFQTAVVETLVIKCRRALKQTGLKNLVIAGGVSANQALRGGLEKMLGEMKGQVFYARPRFCTDNGAMIAYAGCQRLLAGQHDGPAIAVQPRWPMESLPAV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a fast turnover rate for adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP +   H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA.2. For general function, the protein need meet that Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB3. The protein requires Fe(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "MSVTLLAIESSCDETAAAVIQDGNILCNIVASQRIHEKYGGIVPELASRAHQQHIIPVVAQALLEANIQKSDLNAVACTSGPGLLGALLVGVSFSKAFASALHIPVIKVNHMKAHILAHFIGDVKPSFPFICMTVSGGHTQLVIVRNYLEMEVVGETQDDAVGEAFDKTAKLMGLPYPGGPLIDSYAKQGNPLAFPFPTVDMPGYNYSFSGIKTAFMYFLKKNTAVDPDFIQKNLPDICASVQHALIDVLMRKLKRLVVDTGINRVAIAGGVSANSGLRKAMEQKREQEGWDVYIPAFEYCTDNAAMIAVAGYHQYLENDFAGWDLSPEPRLRIG",
    "response#2": "MLILGIETSCDDTGAAIYDLEKGLIIHKVISQNNIHSKYGGVVPEKSSKYHLKNIQPLVENIFKNSNISLSKIDGIAYTAGPGLVGSLIIGATFACSLAYTLQIPSIAINHLEGHLLTPMIKYKRPKFPFLGLIISGAHTQFVLAEDIGKYKIIGDCLDDALGEAFDKVAKLLGIKYPGGKKLSIIAKQGNSKRFFFPRPMTKKPGINFSFSGLKTYAKNLVSSFSKIDNQTKCDIARAFEDSIIDTVIIKCKRALDITNSKILLISGGVSANEPLRKNLRNLMKSRNGKLFFSKKSLCTDNAAMIAYVGSIRFKKNKTKDLSVLINPKWSLEDL",
    "response#3": "MLVLGIESSCDETGVALYDTDAGLRAHALYSQIAMHRDYGGVVPELASRDHIRRVIPLLEDVLAEAGVGRADIDAIAYTKGPGLAGALLVGASVANALGFALGKPLVGVHHLEGHLLSPLLEADRPAFPFLALLVSGGHTQLMRVDAVGQYTLLGETLDDAAGEAFDKTAKLLGLGYPGGPAVSRLAEFGNPGAFELPRPMLHSGNFDFSFAGLKTAVLTQVRKLNLDGGEACEQPRADLARAFVDAIVDVLVAKTLRAAREHGLKRIVVAGGVGANRQLRERLNAEGGKRGLRVYYPDLQFCTDNGAMIAFAGAMRLQADPGQVQSGYGYGVTPRWDLEDL"
  },
  {
    "reference": "MKKKSINKKVKYIFVTGGVLSSLGKGIASASIGTLLESCGLKITILKLDPYINVDPGTMNPFQHGEVYVTHDGAETDLDLGHYERFTHARMGKLNNVTAGRIYHDVIAKERRGEYLGATVQVIPHITDEIKSHIWAVSKGVDVVICEVGGTVGDIESLPFLEAIRQFRFDTNPRNLLYAHLTLIPYIKTADELKTKPTQHSVQKLREIGIQPDILICRTERKVSLELKKKIALFCNVDVESVITAMDAKSIYQVPLNLYEEGLSRIILEKLGLPAKEPDLKQWELIVQNIEKPQDEVKIAIVGKYTALKESYKSIGEALVHGGISNHAKVTMNWIPAETLETDEGKDVLNGCDGILVPGGFGDRGFEGKVQAAKFARINKVPYFGICLGMHSAVVEFARSEGQIKGAHSAEFDPESSDPIIDLMPDQNQNGNKGGTMRLGEYPCQLRKNSFAFQAYGIREIKERHRHRYEFNNKYRFQLEEAGMRFSGISPNGNLVEIIELADHPWFLAGQFHPEFNSSPREPHPLFKAFIAHALEYKNSHQS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein have CTP synthase N-terminal, Glutamine amidotransferase domains with specific sequence motifs that are crucial for its activity.2. The L-glutamine, CTP, UTP binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein should have ATP binding, glutaminase activity, metal ion binding, CTP synthase activity to facilitate 'de novo' CTP biosynthetic process, glutamine metabolic process.4. A protein should be able to manipulate the activity of enzymes in the CTP biosynthesis via de novo pathway pathway, either by activating or inhibiting their function.5. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.6. The Nucleophile; for glutamine hydrolysis, active site, Nucleophile active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "KYIFVTGGVVSSLGKGIAAASIGCLLESRGLRVTLQKFDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTYAPLTQSNNVTSGRIYETIISRERRGDYLGKTVQVIPHVTDEIKANIEKVSGGVDVVLVEIGGTVGDIESLPFLEAIRQLRHKKGRENCFFVHVTLVPWIAAAGELKTKPTQHSVKELRTIGIQPDMLICRCEKPLAGDIKDKIALFCDVSADAVIEACDAKSVYEVPLGFASQNVDDIILRQFKLETPKADLSEWQNMVHKIKSPKPHVHIAXVGKYTGLSDSYLSVIKALQHSSFKVNRKLVIDWIESAELDEETAGTDPEAHXAAWELLKAADGILVPGGFGIRGIEGKIKAAEYARESGTPYLGVCLGLQVATIEFCRNVLGLERANSTEFXEDTPNPAVVFMPEISKTHMGGTMRLGTKPTPFLVDDCKMRRLYDGVNHVDERHRHRYEVNPDLIGQIESAGLKYVGKDETGERCXIMELEGHPYFVGTQYHPEFKSRPDKPSPPYLGLFSAAYPIAEGELKDAYKSLQEALVHGGIANNVRVNITWLASEIFEQDHEAILALEDVNAILVPGGFGERGTSGKIAAVQFARERKVPYLGICYGMQMAVIEAARNLAGLKDANSTEIGPTRNPVVGLMTEWVRGNRTELRQEGGDLGGTMRLGAYDAVLSEGSRVAEIYGTRLISERHRHRYEVNPAYRRHLEEHGVLFSGMSPDGILPEVIEIKDHPWFIGVQFHPELKSKPLDPHPLFSSFVSATVE",
    "response#2": "MTKYVFVTGGVVSGLGKGITAASLGRLLKNRGLKIAAQKLDPYMNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDEDLNKFSNMTSGKVYWNVLTKERNGEYLGSTVQIIPHVTNEIKSFIYAVGRKTNADVVITEIGGTIGDIESQPFLEAIRQVSREVGESNCLFIHVTLVPYLRSSGEHKSKPTQHSVKILQGSGITPHIIVTRSDEPLDQSTKDKIALFCNVKRDCVIENRTVPVLYQAPVMLASEGLDEIVCREMNIDVKPADLTVWNDVLDRLKESYKSLIESLIHCGAHLDTRVEICWVDSEDIEAKGAEALLADCDAVLVAGGFGNRGVEGKIQAIEYARVEKLPFLGICLGMQLSLVEYARNVLGLEDANSIEFNPETTNPLIYLIDNFINQSGAKEIRTHKSPMGGTLRLGEYPCDTKAKSHLREAYGEKTIHERHRHRYEANPAYREQLEKAGMIVTGESHGLIEAVEIKDHPWFLGVQFHPEFTSRLTTPNPEKARKLVGSVRLKDAYKSLQEALVHGGIANNVRVNITWLASEIFEQDHEAILALEDVNAILVPGGFGERGTSGKIAAVQFARERKVPYLGICYGMQMAVIEAARNLAGLKDANSTEIGPTRNPVVGLMTEWVRGNRTELRQEGGDLGGTMRLGAYDAVLSEGSRVAEIYGTRLISERHRHRYEVNPAYRRHLEEHGVLFSGMSPDGILPEVIEIKDHPWFIGVQFHPELKSKPLDPHPLFSSFVDSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASFV",
    "response#3": "KYVFVTGGVVSGLGKGITAASLGRLLKNRGLKIAAQKLDPYMNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDEDLNKFSNMTSGKVYWNVLTKERNGEYLGSTVQIIPHVTNEIKSFIYAVGRKTNADVVITEIGGTIGDIESQPFLEAIRQVSREVGESNCLFIHVTLVPYLRSSGEHKSKPTQHSVKILQGSGITPHIIVTRSDEPLDQSTKDKIALFCNVKRDCVIENRTVPVLYQAPVMLASEGLDEIVCREMNLSDKQPDLSQWKEFLKRHKNPKSSLDAYKSIMESFVHAGAENNCKVVVKPLSAEQVETEEPEKLFSNVSGILVPGGFGERGIEGKIKAVQYARENKIPFFGICLGLQCAVIEFARNVCGIKNAHSSEFKNTKYAVIDLMPEQKNIKNMGATMRLGAYPCVVKEKTNAFEAYKTAYISERHRHRYEVNNKFRSVLTEHGMILSGLSPDKELVEIIELPNHPWFLACQFHPELKSRATNAHPLFREFVVEACKK"
  },
  {
    "reference": "MNGQNGFTVAVVGATGAVGQQMIETLVKRDFPIKKLVLLSSSRSAGKKITVNDVEHTVQEAKPESFEGIDIALFSAGGSVSKELAPEAAKRGAVVIDNTSAFRMDLEVPLVVPEVNEEDVHTHKGIIANPNCSTIQMVVALEPIRKKFGLKKIIVSTYQAVSGAGAAAIEELREETKAIIEGKDFEPKVLPVKASDKHYQIAFNAIPQIDKFEDNGFTFEELKMINETKKIMHDTSLQVAATCVRLPVEVGHSESVYFEIESERVTAAEIRELLSDAPGVVLQDDPANQIYPMPADCVGKNDVFVGRVRKDLDNDNGFHMWVVSDNLLKGAAWNSVQIAESLIRLGVVTAK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be able to modulate isoleucine biosynthetic process in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "GEVYLVGGGPGDPDLLTFKALRLMQQADIVLYDRLVSPQVLDKVRRDAELVYVGKRAGNHAVSQDNINLKLVEYAQQGLRVLRLKGGDPFIFGRGGEEIATLAEYNIPFQVVPGITAASGCASYAGIPLTHRDHAQSVRFIAGHLMSGELNLDWAELAKPAQTLVFYMGLSGMETICSQLMAHGAPGGRAAAIIEKGTQPDQRNFVADLATLPALVRAAQAGAPTLLIVGEVVRLRP",
    "response#2": "GEVYLVGGGPGDPDLLTFKALRLMQQADIVLYDRLVSPQVLDKVRRDAELVYVGKRAGNHAVSQDNINLKLVEYAQQGLRVLRLKGGDPFIFGRGGEEIATLAEYNIPFQVVPGITAASGCASYAGIPLTHRDHAQSVRFIAGHLMSGELNLDWAELAKPAQTLVFYMGLSGMETICSQLMAHGAPGGRAAAIIEKGTQPDQRNFVADLATLPALVRAAQAGAPTLLIVGEVVRLR",
    "response#3": "GEVYLVGGGPGDPDLLTFKALRLMQQADIVLYDRLVSPQVLDKVRRDAELVYVGKRAGNHAVSQDNINLKLVEYAQQGLRVLRLKGGDPFIFGRGGEEIATLAEYNIPFQVVPGITAASGCASYAGIPLTHRDHAQSVRFIAGHLMSGELNLDWAELAKPAQTLVFYMGLSGMETICSQLMAHGAPGGRAAAIIEKGTQPDQRNFVADLATLPALVRAAQAGAPTLLIVGEVVRLR"
  },
  {
    "reference": "MVKIGVLGLQGAVREHVKSVEASGAEAVVVKRIEQLEEIDGLILPGGESTTMRRLIDKYAFMEPLRTFAKSGKPMFGTCAGMILLAKTLIGYEEAHIGAMDITVERNAFGRQKDSFEAALSIEGVGEDFVGVFIRAPYVVEVADNVEVLSKHGDRMVAVRQNQFLAASFHPELTDDHRVTAYFVEMVKEAKMKKVV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate; The resulting ammonia molecule is channeled to the active site of PdxS.2. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).3. The designed protein should have pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity, glutaminase activity within glutaminase complex, cytosol to regulate pyridoxine metabolic process, pyridoxal phosphate biosynthetic process.4. The designed protein should have a high specificity for L-glutamine binding, with minimal off-target effects.5. A protein that can modulate the overall flux of metabolites through the pyridoxal 5'-phosphate biosynthesis, Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MAXYILDAXDFAXAXDGQTXPXAXAXGAYXAXGXDXPXAXAXAXA",
    "response#2": "MGYMTALNFGPQHPAAHGVLRMICELDGEIVERADPHIGLVRLSQKYIGQHQSWDQICYLDRLSYLNNLEHALAVEKLLGITVPVRAKYIRTMFSEITRILNHLLGLTTHALMMFGASALQPYAFERDDVMKLCDFFASGSRRLSDYVYLGGVARQIEARIAEDVLEGFDVSDFETLSQRMLDARPTCLSRIDHS",
    "response#3": "MSKSLSTIEEAIEDIRQGKMIVVVDDEDRENEGDLTMAAEKVTPEAINFMARYGRGLICLTLTEEKVNELQVPMMVSENTSRFETAFTVSIEAREGVTTGISAHDRARTIQVAIDPKSKPEDLARPGHIFPLRAREGGVLVRAGQTEGSVDLARLAGLYPAGVICEIMNDDGTMSRMPELMEFARKHQLKIISIADLIAYRMRKELLVRKEAFLPVKIGPIRIQSKENTYLKAKKESMGHTLPSVESVELEYQTEGTPYIHLKADKWGHLHTYLQKKGKHLHIR"
  },
  {
    "reference": "MSEQEKVEQEEISAELETQNEQEKPMEETEIQDGDALENAIARVQELEEQLIQAVKKEQDILLRTRAEIDNIRRRAEQDVEKAHKFALEKFAKDLLETIDNLERALSTPANVENETIKSLVDGVELTLKGLLSTVARFGVEPVGVIGETFNPELHQAISMQPTEGFESNQITVVLQKGYLLNGRVIRPAMVMVAQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be able to perform chaperone binding under specific conditions.2. The protein must exhibit the following characteristics: Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.; Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. The designed protein sequence is ",
    "response#1": "GANKTIEITVEADCRVCHGKGAVSNQDVITCKRCGGNGQIITEQRTFLGNIRSRQVCPNCSGKGQEIKNKCYACHGQKRQKVC",
    "response#2": "KVEMTISFMDAVLGANKTIEITVEADCRVCHGKGAVSNQDVITCKRCGGNGQIITEQRTFLGNIRSRQVCPNCSGKGQEIKNKCYACHGQKRQKVKQSATFNIPAGIQEGMSLQVPGKGA",
    "response#3": "KVEMTISFMDAVLGANKTIEITVEADCRVCHGKGAVSNQDVITCKRCGGNGQIITEQRTFLGNIRSRQVCPNCSGKGQEIKNKCYACHGQKRQKVKQSATFNIPAGIQEGMSLQVPGKGY"
  },
  {
    "reference": "MRSAHTVSVVREAEAALLARLPEGALMRRAAFGLAVECARLLPRVYGARIVLLVGGGDNGGDALHAGAFLAARGASVFAVLAGRSVHQGGVEALRAAGGKAYAFSGDGSVLFDADLIVDGLVGIGGRGRLREPHATLAVLAANAPAPVVAVDLPSGVDADTGEVAGHAVRADLTVTFGTHKPGLFVDPGAERAGRVVFVDIGLGPELPAPALVRPQAVDIAALLPRPGAESDKYRRGVLAVRAGSDRYRGAAVLTVGGALRTGVGMVRYEGGERAVDQVLARWPETVASVLEPLHSTNLGPGRVSAVAIGPGRGTHRANADELGAVLESEVSVLADADAITLLSGEDGRDLADLVREREAPTLLTPHAGELARLLGADRADVEARRLEHAVRAADDLGCTVLLKGSTTVIASPGAPVVANPTGTSLLATAGTGDVLSGAAGALLAGGLSAANAAMCAAYLHGRAAVLARGGAPISASDLLEGLPLAIREVGEAVA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be able to perform NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity under specific conditions. The designed protein sequence is ",
    "response#1": "VVMGGSIPYLGAATMAGLGAVASGAALVTLSVHSSLAKEARMGYPGLMVASGNPEAPGWPRGKVASLVIGPGWGDEGLAYWFAVVMGDGPWCGLPTVFDADALNILAHDPGLRACDLPLNPSILTPHTGEAARLLAMSAEQVLENRVAAARVLAEKHRAVVVLKGPSTLIAAPDGRLAVNPTGHPLLAQGGTGDVLAGIIGGLQAQGMASFEAACCGTFLHGWAAERLAEHQGESGLRPELLAI",
    "response#2": "MDQHKGQGGHTVVMGGSIPYLGAATMAGLGAVASGAALVTLSVHSSLAKEARMGYPGLMVASGNPEAPGWPRGKVASLVIGPGWGDEGLAYWFAVVMGDGPWCGLPTVFDADALNILAHDPGLRACDLPLNPSILTPHTGEAARLLAMSAEQVLENRVAAARVLAEKHRAVVVLKGPSTLIAAPDGRLAVNPTGHPLLAQGGTGDVLAGIIGGLQAQGMASFEAACCGTFLHGWAAERLAEHQGESGLRPELLS",
    "response#3": "MDQHKGQGGHTVVMGGSIPYLGAATMAGLGAVASGAALVTLSVHSSLAKEARMGYPGLMVASGNPEAPGWPRGKVASLVIGPGWGDEGLAYWFAVVMGDGPWCGLPTVFDADALNILAHDPGLRACDLPLNPSILTPHTGEAARLLAMSAEQVLENRVAAARVLAEKHRAVVVLKGPSTLIAAPDGRLAVNPTGHPLLAQGGTGDVLAGIIGGLQAQGMASFEAACCGTFLHGWAAERLAEHQGESGLRPELLR"
  },
  {
    "reference": "MENEKENLFFEPHKRGLMKTPLKESTAANIVLADIQPDFGPLTTPTKPKEISQGEPWTPTANLKMLISAVSPEIRNRDQKRGLFDNRNGLSDVKDCLHEHFSGDEYEKSQPSRKEKSLGLLCHKFLARYPNYPNPAVNNDICLDEVAEELNVERRRIYDIVNVLESLHMVSRLAKNRYTWHGRHNLNQILETLKSVGEENKYAEQIMMIKKKEYEQEFEVSKSYNTEDPIIKSNTGQNGHPDMCCAERPGVELRAASVNSRKDKSLKVMSQKFVTLFLVSTPQIVSLEIAAKILTWEDHVEDLDRSKFKTKIRRLYDIANVLSSLDLIKKVHVTEERGRKPAFKWTGPEISPNPSGLSPVLPCAASDLEARQSSKENCAKNLFSTRGKPNFTRHPSLIKLVKSIESDRRKINSAPSSPIKTHKAESTQNSVPFRSKMAQLAAICKMQLEEQSSEPRKNVTVQLAGSGHCKSVAPLDTPANAEPEMMAPSLIQPLGVVPLLPSPLSPAVPVILPQTPSGTSYAIYLQPAQAQTITPPPGLSPTVCPTTSSNAMISEDSTDATGENADSDAPKSSVSTRPGSLLPGPERQGAKNREREPAREKGSKRASMLEDSGSKKKFKEDQKAPENVSTTLFPSGYLIPLTQCSTLGAESILSSNENSGTLSPNHSIYSSPIAGVIPVTSSELTAVNFPSFQVTPLKLMVSPTSMAAVPVGNSPALSSSHPLPIQNPSSAIVNFTLQHLGLISPGVQVSTSPGPGTIAVSPRIEAVS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that can perform DNA-binding transcription repressor activity, DNA-binding transcription factor activity, RNA polymerase II cis-regulatory region sequence-specific DNA binding, DNA-binding transcription factor activity, RNA polymerase II-specific in RNA polymerase II transcription regulator complex to support cell cycle comprising mitosis without cytokinesis, chorionic trophoblast cell differentiation, negative regulation of cytokinesis, sprouting angiogenesis, regulation of transcription by RNA polymerase II.2. For general function, the protein need meet that Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'; Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene .; Directly represses transcription of classical E2F transcription factors such as E2F1: component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis; Required for placental development by promoting polyploidization of trophoblast giant cells3. A protein with DNA binding capability for targeted gene regulation.4. The designed protein's functionality will benefit from Basic and acidic residues, Polar residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "GRFTLEPATNCSNSLWAEPLFSLVSGLVGINPPPASIPSSTSQATHPSSSSTSSIPSSSSSSTSSASLSCSVHQSEPNPIYSAAPTYSSASPDIFPESGPNFSTTVGTSLQYSGIHPDSPATGEQWMAKPVASKLRLVEFSAFLEQQRDPDSYNKHLFVHIGHANHSYSDPLLESVDIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLNCNIQDDAGAFYGVSSQYESSENMTVTCSTKVCSFGKQVVEKVETEYARFENGRFVYRINRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETLLCMACVFEVSNSEHGAQHHIYRKLRLVEFSAFLEQQRDPDSYNKHLFVHIGHANHSYSDPLLESVDIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLNCNIQDDAGAFYGVSSQYESSENMTVTCSTKVCSFGKQVVEKVETEYARFENGRFVYRINRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETLLCMACVFEVSNSEHGAQHHIYRKLRLVEFSAFLEQQRDPDSYNKHLFVHIGHANHSYSDPLLESVDIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLNCNIQDDAGAFYGVSSQYESSENMTVTCSTKVCSFGKQVVEKVETEYARFENGRFVYRINRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETLLCMACVFEVSNSEHGAQHHIYR",
    "response#2": "LEAKELWDQFHKLGTEMVITKSGRRMFPPFKVRVSGLDKKAKYILLMDIVAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMAKPVAFHKLKLTNNISDKHGFTILNSMHKYQPRFHIVRANDILKLPYSTFRTYVFPETDFIAVTAYQNDKITQLKIDNNPFAKGFRLRRGVCTRVYTTTPKKPNSALRKVARVRLTSGFEITAYIPGIGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGTLDTVGVKDRKQRRSKYGAKDPKVTLEAKELWDQFHKLGTEMVITKSGRRMFPPFKVRVSGLDKKAKYILLMDIVAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMAKPVAFHKLKLTNNISDKHGFTILNSMHKYQPRFHIVRANDILKLPYSTFRTYVFPETDFIAVTAYQNDKITQLKIDNNPFAKGFRDTGNGRKDKSLRIMSQKFVMLFLVSKTQTVTLDMAAKILIEEGQEESYDSKYKTKVRRLYDIANVLTSLNLIKKIHMREEKTRKPVFKWILEAKELWDQFHKLGTEMVITKSGRRMFPPFKVRVSGLDKKAKYILLMDIVAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMAKPVAFHKLKLTNNISDKHGFTILNSMHKYQPRFHIVRANDILKLPYSTFRTYVFPETDFIAVTAYQNDKITQLKIDNNPFAKGFRLEAKELWDQFHKLGTEMVITKSGRRMFPPFKVRVSGLDKKAKYILLMDIVAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMAKPVAFHKLKLTNNISDKHGFTILNSMHKYQPRFHIVRANDILKLPYSTFRTYVFPETDFIAVTAYQNDKITQLKIDNNPFAKGFR",
    "response#3": "AAGLDAGGLGPAASAASTAAPFPFHLSQHMLASQGIPMPTFGGLFPYPYTYMAAAAAAASALPATSAAAAAAAAAGSLSRSPFLGSARPRLRFSPYQIPVTIPPSTSLLTTGGIHPDSPATGEQWMAKPVAGKLRLVEFSAFLEQQRDPDSYNKHLFVHIGHANHSYSDPLLESVDIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLNCNIQDDAGAFYGVSSQYESSENMTVTCSTKVCSFGKQVVEKVETEYARFENGRFVYRINRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETLLCMACVFEVSNSEHGAQHHIYRLGTFQFSSKHKEALAHRWGLIHPDSPATGEQWMAKPVAGKLRLVEFSAFLEQQRDPDSYNKHLFVHIGHANHSYSDPLLESVDIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLNCNIQDDAGAFYGVSSQYESSENMTVTCSTKVCSFGKQVVEKVETEYARFENGRFVYRINRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETLLCMACVFEVSNSEHGAQHHIYRVTGQYRMLAKKGGYVWVETQATVIYNSKNSQPQCIVCVNYVLSEHDPKVTLEAKELWDQFHKLGTEMVITKSGRRMFPPFKVRVSGLDKKAKYILLMDIVAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMAKPVAFHKLKLTNNISDKHGFTILNSMHKYQPRFHIVRANDILKLPYSTFRTYVFPETDFIAVTAYQNDKITQLKIDNNPFAKGFRDTGNG"
  },
  {
    "reference": "MVAKKPRVVVVGAGISGLAAAHRLCGAGGDRFEVAVVEAGDRVGGRILTSEFAGHRVEMGATWVQGVVGSPVYALARDAGALGEEEGRGLPYERMDGFPDRVLTVAEGGEVVDADTVAGPIEELYRGMMEAARAGEAGGGGGVEEYLRRGLRAYQAARSAGGGGGGGKELEEVDEALLAMHINRERTDTSADDLGDLDLTAEGEYRDFPGEHVTIPGGYSRVVERLAAALPPGTVRLGLRLRRLKWGGTPVRLHFADGAPPLTADHVILTVSLGVLKASLGNKDTAGVGAAAIAFDPPLPPFKREAVARLGFGVVNKLFMEVEAVAPSEPEDVAGVQPAAAGFPFLHMAFRGHVSKIPWWMRGTESICPVHAGSTVALAWFAGREAAHLESLPDDDVIRGAHATLDSFLPAAPRWRVRRIKRSGWATDPLFLGSYSYVAVGSSGDDLDRMAEPLPRGPDAAADERPPSPRLLFAGEATHRTHYSTTHAAYLSGVREANRLLQHYRGGANHTT",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Plays an important role in the regulation of polyamine intracellular concentration (Probable).2. The FAD binding site should be stable and able to withstand changes in environmental conditions.3. We are looking for a protein with broad substrate specificity for H2O + O2 + thermospermine = 3-aminopropanal + H2O2 +   spermidine.4. The protein design must be able to perform polyamine oxidase activity in order to contribute to regulation of timing of transition from vegetative to reproductive phase.5. The protein requires FAD to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "MKNEQVVHDFLQAVCIDGIAVLKGAPQGVRGAVEAIGDRIGMIKRTHFGLVFEVSLKADASNMAYASNGGLPFHTDFPSLSHPPQLQMLHMLQSAEEGGHSLFVDGFHVAEQLRVEKPEIFKILTTQSMEYIEEGYDVHEINGKTIRFDYDMCARHKVIRLNDDGKVNKIQFGNAMRSWFYDCEPSKVQDVYRAMKTFTEYCYQPRNMLKFRLEDGDTVLWANQRLLHTRDGFRNAPEKARTLTGCL",
    "response#2": "MKNEQVVHDFLQAVCIDGIAVLKGAPQGVRGAVEAIGDRIGMIKRTHFGLVFEVSLKADASNMAYASNGGLPFHTDFPSLSHPPQLQMLHMLQSAEEGGHSLFVDGFHVAEQLRVEKPEIFKILTTQSMEYIEEGYDVHEINGKTIRFDYDMCARHKVIRLNDDGKVNKIQFGNAMRSWFYDCEPSKVQDVYRAMKTFTEYCYQPRNMLKFRLEDGDTVLWANQRLLHTRDGFRNAPEKARTLTGCI",
    "response#3": "MKNEQVVHDFLQAVCIDGIAVLKGAPQGVRGAVEAIGDRIGMIKRTHFGLVFEVSLKADASNMAYASNGGLPFHTDFPSLSHPPQLQMLHMLQSAEEGGHSLFVDGFHVAEQLRVEKPEIFKILTTQSMEYIEEGYDVHEINGKTIRFDYDMCARHKVIRLNDDGKVNKIQFGNAMRSWFYDCEPSKVQDVYRAMKTFTEYCYQPRNMLKFRLEDGDTVLWANQRLLHTRDGFRNAPEKARTLTGCR"
  },
  {
    "reference": "MIQISALPAFTDNYIWLLQDHATQRCAVVDPGDAAPVQAWLDAHPGWVLGDILITHHHHDHVGGVETLKKATGATVYGPASENIPGRDVALKDNDTVRVLGWDFDVYAVPGHTLGHIAYYHHGLLFCGDTLFAAGCGRLFEGTPQQMHQSLSRLAALPEDTLVYCTHEYTLSNLKFAAAVEPDNRDIAARLEKVTRQRENGVMTLPSTLALEKLTNPFLRTGEILVTQKVDERNGPQNRAPSEVFAALRAWKDTF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should take into account the physiological conditions of the methylglyoxal degradation, Secondary metabolite metabolism, (R)-lactate from methylglyoxal: step 2/2 pathway.2. The designed protein should be optimized for hydroxyacylglutathione hydrolase activity, metal ion binding activity in order to improve methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione.3. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding.4. We are looking for a protein with broad substrate specificity for an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy   carboxylate + glutathione + H(+).5. The presence of Zn(2+) is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "MEYTLSNAAFALHVDPTNTALQKRRQQANAMRLAGLPTLPISLESELNTNPFLRTAAPTIHAATATHLQRTPIDEVEVFATLRHWKNNEYTVSNAVFARHVDPANAALWQRQEEALAMRRDDRSTLPVTLANEFDCNPFLRVHAAPIRASVSAHLGRDVVDDVDVMAGLRHWKDGEYTVSNAVFARHVDPANAALWQRQEEALAMRRDDRSTLPVTLANEFDCNPFLRVHAAPIRASVSAHLGRDVVDDVDVMAGLRHWKDGFL",
    "response#2": "MNYIWTLIAPDGRAIIVDPGQALPILEAHSKGLIPTAILLTHHHADHIGGVPELLERWPTLPVYAPHDTRIALNYHRIGEGDSLNILGMRFQVIHTPGHTHSHLTFIGNDLLFCGDTLFSLGCGQIFEGTPTQMLASLQRLAALPIQTRVCCGTEYTLANLHFAKAVEANNPALIEYTVKAHALRAENKPTLPSSIALEKAINPFLRLDSLEIQKSLAVQFQQPIADPVQSFALLRQWKDNEYTLANLAFAKYILPENVAIQQHEQWVQKQRVTHQPSLPTTMGREKQINPFLIAQDMETFVAWRKAKL",
    "response#3": "MQKNYIWTLIADDGTAVVVDPGDAAPVLALADQGLRVDTILLTHHHDDHIGGVPALQARFPGVRVIAPVEERIPTATGRVGEGERVQALGRTFHVLSVPGHTRSHIAFHTAEHLFSGDSLFSLGCGRLFEGTPSQLLASMRKLGTLPAQLLLCCAHEYTKSNALFALTLDPYNQKLQQRAEDVFLLRAKNAMTLPVTLGQEKKNNPFLRWDNRTLRKTLRMEKETDEEVFAEIRKRKDNFK"
  },
  {
    "reference": "MARIVAEQFVETLAAAGVKRIYGIVGDSLNGLTDAVRRHGKIEWLHVRHEEVAAFAAGAEAHLTGELAVCAGSCGPGNLHLINGLFDCHRSRVPVLGIAAHIPSPEIGSGYFQETHPQILFQECSHYCELIAGARQMPRVLDVAIREAVGKRGVSVVVIPGDVALQPATDAPAPKAGGLLPRAPVVTPSPGDLDRLAALLNDEDDRITILCGSGCQGAHHELLALGARLKAPMVHALRGKEHVEWENPYDVGMTGLIGFSSGYYAMRDCDVLLMLGTDFPYRQFYPEGNAVRIAQVDLRPENIGRRVPVDLGVVGDVRATLTALLPLLKQKRDGKHLSQAQQHYAKSRRELDDLAKGTSGKRLIHPQQIAKALSDQAAADAIFTCDVGLPTVWAARYLAMNGKRRLLGSFWHGSMANAMAQAIGAQAVYPGRQVISLSGDGGFTMLMGDFISLTQLKLPVKVVVFNNGTLGFIELEQKSTGFLEFGTELNNPNFAAMAKAMGIYGVRLEDPADVEPGIAAALAHDGPVLIDAVVNRTELAMPPSITLEMAKGFTLYMVKAIMSGRGDEIIDLAKTNLWR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must be optimized for ubiquinone binding, lipid binding. The designed protein sequence is ",
    "response#1": "LQDRVAPFDGALARKHIELAMGGPLETWFDDFDQQALASASIAQVHTARLKTTGQEVVLKVIRPDIRPIIKADVRLMYRLAAWVPKLMPDGRRLRPREVVREYEKTLLDELNLLREAANAIQLRRNFDGSPMLYVPEVYSDYCRESVLVMERIYGIPVSDIPTLEKQGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVSYEHPEDPCYIGIDCGIVGSLNKDDKRYLAENFIAFFNRDYRKVAELR",
    "response#2": "LQDRVAPFDGALARKHIELAMGGPLETWFDDFDQQALASASIAQVHTARLKTTGQEVVLKVIRPDIRPIIKADVRLMYRLAAWVPKLMPDGRRLRPREVVREYEKTLLDELNLLREAANAIQLRRNFDGSPMLYVPEVYSDYCRESVLVMERIYGIPVSDIPTLEKQGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVSYEHPEDPCYIGIDCGIVGSLNKDDKRYLAENFIAFFNRDYRKVAELS",
    "response#3": "LQDRVAPFDGALARKHIELAMGGPLETWFDDFDQQALASASIAQVHTARLKTTGQEVVLKVIRPDIRPIIKADVRLMYRLAAWVPKLMPDGRRLRPREVVREYEKTLLDELNLLREAANAIQLRRNFDGSPMLYVPEVYSDYCRESVLVMERIYGIPVSDIPTLEKQGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVSYEHPEDPCYIGIDCGIVGSLNKDDKRYLAENFIAFFNRDYRKVAELY"
  },
  {
    "reference": "MTKVLVEPDPEEVKQLSEALGRQPVILAGICEAEYRGRAESVAGPALRIAMCKPDGTFILHNAMEKREPTNWNPAPSRQSIEVRDGCVVLRSRRLDVPEEVVVYFHKVLLACSLPKEGAKSEDSVFSLFRSEEDMKRVIREDPSVIEPGFRPVGEEVECGAGVADVVGYDEEGRFVVLELKRTRAGVSAASQLRRYVEAFREERGEEVRGILVAPSVTDRCRRLLEKYGLEWKKLEPVPLRDDGGKKQCTLTEFLAGEGD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Cleaves both 3' and 5' ssDNA extremities of branched DNA structures..2. The protein must have high specificity towards single-stranded DNA endodeoxyribonuclease activity. The designed protein sequence is ",
    "response#1": "RGKKEIEKLVDTGAMTPLVHVSGMLGNYRGNCTWVAPLAWHPTNQNALIVCDLTGDIDNLLAKSADELRADLYTKKLELEERGVSSVPLKLVHINKCPILAPAKTLLPETANRLGIDRQLCLDNLAKLRASFDIREKVADIFNEERQFASNDNVETELYNGFFSNADKNNMAILRSLPAEKLSEHGLAFEDKRILELLFHYRARHFYKTLTRAEQIKWKKYRQNKLEKSAVEFEASLQRLVEXHSDNSEKLSLLQQVYEYL",
    "response#2": "RGKKEIEKLVDTGAMTPLVHVSGMLGNYRGNCTWVAPLAWHPTNQNALIVCDLTGDIDNLLAKSADELRADLYTKKLELEERGVSSVPLKLVHINKCPILAPAKTLLPETANRLGIDRQLCLDNLAKLRASFDIREKVADIFNEERQFASNDNVETELYNGFFSNADKNNMAILRSLPAEKLSEHGLAFEDKRILELLFHYRARHFYKTLTRAEQIKWKKYRQNKLEKSAVEFEASLQRLVEXHSDNSEKLSLLQQVYEYN",
    "response#3": "MPRTLTDPTADRAAAFATDAAADGLTVSLIGSCVATLTGRSQRDLAAGVRTLLWKPDDTVVVHGD"
  },
  {
    "reference": "MPRDHACRARRSFEIGRYAEWMAAPLPSITDVDQTALAAWLAERGEPPYRVGQIRHGVARSTAADWAEVTDLPHELRAELAGSFRWTSVALETEIASADGETRKALLRLHDGHHVETVLMPHHGARNSACISTQAGCPMACAFCATGEMGLVRDLTAGEIVDQVRHWQRELRQRGDRVSHVVYMGMGEPLHNYGPSLAAVRMLTDPELFGISPRRVTISTCGIVPKMDALAAEGLPVNLAVSLHAADDRTRSQIMPVNRKWGVDDVLDASARYVERTKRRLTFEYLLLAGVNDSPEDAERLGRRIAARGRTAMYHVNLIPVNPGPGGFKRPSPERMERFAEVLEQHGVAATVRISKGQDIKAGCGQLKVAEGRAAAVRLERATPGRV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The designed protein should contain one or more Radical SAM core domains that are essential for its function.3. The protein should have a high affinity for [4Fe-4S] cluster, S-adenosyl-L-methionine.4. A protein that can perform tRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding, rRNA (adenine-C2-)-methyltransferase activity in cytoplasm to support rRNA base methylation. The designed protein sequence is ",
    "response#1": "MIDILSLYPNEIEEFVISIGEKSFRAKQIFSWLYKGADDFSRMSNLSKTFITKLKQNAEIKSIKIAKKYVSALDGTIKYLFELDDGEKIETVFMRYQHGNTICLSTQAGCKMGCAFCASTINGYKRNLLPSEMILQIMACEKDSGEKVSNIVLMGIGEPLDNYENVVRFLRLVNHNDGLNIGMRHISLSTCGLVDKIDRLAKENFALTLSVSLHATDDAKRREIMPIANRWTIEQLLASCERYIKQTSRRISFEYALIAGVNDSFEDAHKLGRMLAGMLCHVNLIPVNTVAEKKYKKSEKNAIEAFTRAVETYKISVTVRRKLGSDIDASCGQLRASL",
    "response#2": "YKHGNSICISTQVGCRMGCKFCASTLGGRVRDLTCGEILSQVIIGRKICGDRISNIVLMGSGEPLDNFDNVMKFLELVNADYGLNIGQRHITLSTCGIVPKIIELADKEMSITLAISLHAFSDEKRKEIMPIANKYSIIEILDACRYYINKTGRRITFEYSLVSGVNDGKEDARSLAKILKGMLCHVNLIPVNEIKENTLKRPSKKVIEEFEEILKNNGIEVTVRREMGADLD",
    "response#3": "SQIGCALDCSFCATGKQGFNRDLTASEIIGQLWIAADSFNQFSSTTTRRITNVVMMGMGEPLMNFDNVVEAMSIMLDDHAYSLSKRRVTLSTAGVLPALDRLGDVLDVSLAISLHAPNDELRNKLVPLNKKYSINEFIDSARRYLSKMPDSRRKVTVEYTLMDQVNDR"
  },
  {
    "reference": "MRSVEELLSEIVVAERKGAVVTEVSGVESDSRQVKEGYLFVAVRGVSVDGHTFIPQAIAQGAKVVVCEEFPAELNSEVMYVRVDDSSIAFGLLASAWYGNPSRELTLVGVTGTNGKTTTATLLYEMFRSLGYKAGLLSTVCNYIDEVREPATHTTPDPLHLHRMLREMVDAGCQYAFMEVSSHSAAQHRIAGLDFDGAIFTNLTRDHIDYHKTFEAYLKAKKSFFDGLPKGSFALTNIDDKAGMVMLQNTRAEKHTYSLRTMADFRVRIIESRIDGMALEINGKEVEVRFVGKFNAYNLLAVYGATCLLGQDPDEVLRRMSLLVPVAGRFQTLHSSRGYTVIVDYAHTPDALNNVLTSIREVLGNRGHIITVVGAGGNRDKGKRPMMAREAVNLSDRVILTSDNPRFENPNDILNDMLAGLDAEQRRKTLTQVDRREAIRTATQFANPGDVILVAGKGHEDYQEIEGVKHHFDDKEEVEKLFAEEQA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should consider the endogenous proteins and enzymes present in the Cell wall biogenesis pathway to avoid interference with native metabolic processes.2. The designed protein have Mur ligase central domains with specific sequence motifs that are crucial for its activity.3. The protein must bind to Mg(2+) in order to perform its enzymatic function.4. The designed protein should have a binding site that is specific to the target ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate ligand.5. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..6. The protein should have a fast turnover rate for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "MSLKKLITSLPCLDSRQVKQGDIFIALVGTRADGRQFIANAVEQGAAAVFVEADKEWQGLEVEVLEVEDPRRVLARVAARFFPRQPDRVLAVAVTGTNGKTSTVDFLRQILTLSGFKVALLGITGTNGKTTVSTLLYQLFTNLGYKTGLISTIKIMVADQEYATYLTTPDSLAINKYLAEMVEEGVDYCFMEVSSHGIAQHRTTALQFAGGVFTNLSHDHLDYHKTFKEYRDVKKIFFDELSKNAFALTNQDDKNGEFMVQNTIANKKTYALKTNADYKAHILENEISGLLLKINNHEVWTKLIGSFNAYNLTAIFAVSVLSALGVSLEEAIRLVASLPPVPGRMETFTRADGLAVIVDYAHTPDAVALALQAARGATKGRLFAVVGCGGDRDAGKRPAMGAAAARTADVVIVTDDNPRSEDPATIRAAAVEGARAAVPGVELREVGDRREAISTAIGLAGPGDTVVVLGKGHEDYQEVKGERHPFSDREVARTYLEERERGGEG",
    "response#2": "MRLKDILYKVTINAVTGSTSIDVTGFAIDHRKVAPGTIFGAFQGMQVNGEDFVPAAIEAGAIAVVARPSVAVVADDTRYAFALMSSAFYGEPSRQLKLTGVAVTGTNGKTSTVDFLRQILTLSGYNAGLVGTIHTRIGDKEIEASNTTPESYELQKYLNRMVDAGCQCAVMEVSSHALELYRVDGCHFRVGVFTNLTQDHLDFHKTFENYRDAKAKLFLQCDYAVINRDDRYADEMLRESRAKIVRFGIEEKADVVAKDIQLGVDGTSFHVLYKGERYDVKLKMVGRFNVYNALAAISAALLEGIPLEDIKEGLKNVEVAVTGTNGKTSTVDFLRQILTLSGHEDYQILKDRTIHLDDREVVREEIRKMNKGG",
    "response#3": "MTLAELLSPDSRRCAPGVAFFAFPGDAADGRAHIPEALARGAAAVLWEEDGFSWRQEWRVPNAAVRGLRQQAGRLAHEFYGEPPAALHVVGTGTNGKTSCSQLYAQLQALLGKRAAMLGTMGYGEVNRSADIKLQDTGMTTPDALRAQSILADLRETGIQSVAMEVSSHSLVQGRVAAVDFDIAVFTNISRDHLDYHGNMAAYAKAKAKLFAFKGLSAAVLNLDDEYGELFAHVLRASGSKVITYSCKNSLADLYCAKVDYLPRGMVLTIKSSWGAIQLKTGLLGSFNVSNLLAVLGAVHALGVSPEDAARALGASAGVPGRVQRIDRGQNFLAVVDYAHKPAAVEAVLATLRESSRGRIAVVLGAGGDRDTAKRPLMGAAGARGADLLVVTDDNPRSEDPATIRQAVQDGALSVPEDARGEVRNVAGRAAAIEQAVVWAREGDVVLIAGKGHETYQVIGDRVLPFDDREEARRALRRREGA"
  },
  {
    "reference": "MKKVGLIGWRGMVGSVLMQRMREARDFDAIEPVFFTTSQHGQPGPEVGKAVPPLQDAKSIDALKAMDVILTCQGGSYTRDVYPQLRAAGWNGYWIDAASTLRMESESIIVLDPVNRDMIDRGLRDGAKTFVGGNCTVSLMMMALGGLFRRDLIEWITPMTYQAASGAGARNMRELLSQMGSLHASVKERLDDPAAAILDIDRQVTERMRSDAFPVDHWQVPLAGSLIPWIDSELPSGQSREEWKAEVETNKILGRADRPIPIDGLCVRIGAMRSHSQALTIKLRRDLPLDEIHAMLDADNEWVKVIPNEKEATMRELTPAAVSGTLTIPVGRLRKLSMGKEYLSAFTVGDQLLWGAAEPLRRMLRILLEDADGLQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be optimized for NAD binding, protein dimerization activity activity in order to improve lysine biosynthetic process via diaminopimelate, threonine biosynthetic process, 'de novo' L-methionine biosynthetic process, diaminopimelate biosynthetic process.2. The protein design should take into account the physiological conditions of the L-threonine from L-aspartate: step 2/5 pathway.3. The NADP(+), phosphate, substrate binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "LKPLLDNFGVKRVIVSTMQAVSGAGWGGVPSMAIIDNVIPYIAKEEEKVEAESLKILGSLNQGEVKAANFKISASCHRVNVLDGHLEAVFVELGKAVEAVEVINAMKNFKGKPQELKLPTAPQQPIIVKDEPDRPQPRFDRYAGGGMSVAVGRVRRDPAIENGVKYVVLGHNTGG",
    "response#2": "MAKAIAIVGATGSVGEAMLSILDERNFPVGTVHALASSRSVGKRVSFGETHLVVQDLADFDFSQVRIALFSAGAAVSAEYVPKAVEAGCICIDNTSRFRYDEGIPLVVSEVNPERIADAFTARGVRNIVRVAVVGATGMVGQRFVKLLENHSMFELVALTASPASVGKRYSEAAHWYVEGQMPEGVAEMKVSTDDPNWLKALGVEVAFTALPAEVALKVEPELAKAGINVLSDASTFRMEKDVPVLIPEVNPEHLKLLKPLLDNFGVKRVIVSTMQAVSGAGWGGVPSMAIIDNVIPYIAKEEEKVEAESLKILGSLNQGEVKAANFKISASCHRVNVLDGHLEAVFVELGKAVEAVEVINAMKNFKGKPQELKLPTAPQQPIIVKDEPDRPQPRFDRYAGGGMSVAVGRVRRDPAIENGVKYVVLGHNTVYG",
    "response#3": "LKPLHEYGKIKRLIVSTYQAVSGAGKEAIDDLHNGTQAAINKEEYHYKKFTTKPIAFNLIPQIDVFCENGYTKEEMKVVEETKKILHLADDTPISCTAVRVPVFVSHSEAVSVEFEKEITPDLARKILSTAYGVTVIDNPAKSEYPTPIEAAGKDPVYVGRIRKDLALNNGLALWVVADNLL"
  },
  {
    "reference": "MHRLTSDVDFFARSAVQVAADLIGADFTVSGVGGTIVETEAYLPDDAASHSFAGTTARNRAMFGPPAHAYIYLSYGLHWCLNFVCLPGSAVLIRAIEPRWGIDTMRARRGVREERLLCSGPGRVGQALAISRELDGLPLGEDPFRLTLPSTKPPLAAGIRVGITKAVEQPWRFGLAGSSFVSRKF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein with DNA binding, alkylbase DNA N-glycosylase activity is needed to enhance base-excision repair in a specific context. The designed protein sequence is ",
    "response#1": "PKEVLFLGMNPGPFGMAQTGVPFGEVNVVRDWLGVGGPVLTPPQEHPKRPVLGLECPQSEVSGARFWGFFRTLCGQPQVFFRHCFVHNLCPLLFLAPSGRNLTPAELPAKQREQLLSICDAALCRQVQLLGVRLVVGVGRLAEQRARRALAGLTPEVQVEGLLHPSPRSAQANKGWEAAAL",
    "response#2": "PKEVLFLGMNPGPFGMAQTGVPFGEVNVVRDWLGVGGPVLTPPQEHPKRPVLGLECPQSEVSGARFWGFFRTLCGQPQVFFRHCFVHNLCPLLFLAPSGRNLTPAELPAKQREQLLSICDAALCRQVQLLGVRLVVGVGRLAEQRARRALAGLTPEVQVEGLLHPSPRSAQANKGWEAAAL",
    "response#3": "PKEVLFLGMNPGPFGMAQTGVPFGEVNVVRDWLGVGGPVLTPPQEHPKRPVLGLECPQSEVSGARFWGFFRTLCGQPQVFFRHCFVHNLCPLLFLAPSGRNLTPAELPAKQREQLLSICDAALCRQVQLLGVRLVVGVGRLAEQRARRALAGLTPEVQVEGLLHPSPRSAQANKGWEAAAL"
  },
  {
    "reference": "MRERTVTYSPAFTLVPTRECFNRCGYCNFRADRGAAWLQPAEVRALLLPLVGSGVVEILVLSGEVHPHDPRRGEWFAMIEDICAVALELGFLPHTNCGVLSFEEMRALQQLNVSLGLMLEIDSNRLLGGVHRHAPSKIPALRTAQLEWAGALGIPFTTGLLLGIGETPAEREDTLRTIARLQDRHGHIQEVILQPHSPGGSQSWAGEPLGDAQLLGVVRLARQILPAEITIQIPPNLVGDPVPLLEAGARDLGGIGPVDVVNPDYAHPVVERLGERLAAAGWRLEPRLPVYPHLDKRVATALQPLLGEHRARLCQAAVT",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must be able to 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity, 4 iron, 4 sulfur cluster binding.2. A protein that can modulate the overall flux of metabolites through the coenzyme F0 biosynthesis, Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed.3. The protein design should incorporate a binding pocket that specifically recognizes [4Fe-4S] cluster to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "PHVGAMRIAASMHGVHYVLHAPQGDTYADLLFTMIERRGQRPPVTYTTFQARDLGGDTAELVKRHVQEAADRFQPDALLVGESCTAELIQDQPGALAQGMGLPMPVVTLELPAYSKKENWGASETFYQLMRGLLKQSVPPQPSHDVQAWKHEGRRPRVNLLGPSLLGFRCRDDVLEVQRLLSLHGIDVGVVAPLGAGVEDILRLPQADLNVCLYPEVAESSCSWLERNFGIPFSKTVPIGMGATHDFLVEVHALLGMTPPEAAEGYQRSRMPWYSESVDSTYLTGKRVFIFGDGTHAIAAARICSEELGFTVVGLGSYSREMARPVRAAAKKLGLEALICDDYLAVEAAMAEAAPELVLGTQMERHSAKRLGIPCAVISTPMHVQDVPARLSPQMGWEGANVIFDAWVHPSWREPGMYARPHVGAMRIAASMHGVHYVLHAPQGDTYADLLFTMIERRGQRPPVTYTTFQARDLGGDTAELVKRHVQEAADRFQPDALLVGESCTAELIQDQPGALAQGMGLPMPVVTLELPAYSKKENWGASETFYQLMRGLLKQSVPPQPSHDVQAWKHEGRRPRVNLLGPSLLGFRCRDDVLEVQRLLSLHGIDVGVVAPLGAGVEDILRLPQADLNVCLYPEVAESSCSWLERNFGIPFSKTVPIGMGATHDFLVEVHALLGMTPPEAAEGYQRSRMPWYSESVDSTYLTGKRVFIFGDGTHAIAAARICSEELGFTVVGLGSYSREMARPVRAAAKKLGLEALICDDYLAVEAAMAEAAPELVLGTQMERHSAKRLGIPCAVISTPMHVQDVPARLSPQMGWEGANVIFDAWVHP",
    "response#2": "PHVGAMRIAASMHGVHYVLHAPQGDTYADLLFTMIERRGQRPPVTYTTFQARDLGGDTAELVKRHVQEAADRFQPDALLVGESCTAELIQDQPGALAQGMGLPMPVVTLELPAYSKKENWGASETFYQLMRGLLKQSVPPQPSHDVQAWKHEGRRPRVNLLGPSLLGFRCRDDVLEVQRLLSLHGIDVGVVAPLGAGVEDILRLPQADLNVCLYPEVAESSCSWLERNFGIPFSKTVPIGMGATHDFLVEVHALLGMTPPEAAEGYQRSRMPWYSESVDSTYLTGKRVFIFGDGTHAIAAARICSEELGFTVVGLGSYSREMARPVRAAAKKLGLEALICDDYLAVEAAMAEAAPELVLGTQMERHSAKRLGIPCAVISTPMHVQDVPARLSPQMGWEGANVIFDAWVHPAGTPHVCLDVRIALQHDICALEHALHIPLRDLATSDGMAKLRTAARADSPSPLPVYTLCRRGIASRRALVLLDDADFAAPVYNIDGGLKAWKRDVS",
    "response#3": "PHVGAMRIAASMHGVHYVLHAPQGDTYADLLFTMIERRGQRPPVTYTTFQARDLGGDTAELVKRHVQEAADRFQPDALLVGESCTAELIQDQPGALAQGMGLPMPVVTLELPAYSKKENWGASETFYQLMRGLLKQSVPPQPSHDVQAWKHEGRRPRVNLLGPSLLGFRCRDDVLEVQRLLSLHGIDVGVVAPLGAGVEDILRLPQADLNVCLYPEVAESSCSWLERNFGIPFSKTVPIGMGATHDFLVEVHALLGMTPPEAAEGYQRSRMPWYSESVDSTYLTGKRVFIFGDGTHAIAAARICSEELGFTVVGLGSYSREMARPVRAAAKKLGLEALICDDYLAVEAAMAEAAPELVLGTQMERHSAKRLGIPCAVISTPMHVQDVPARLSPQMGWEGANVIFDAWVHPAGTPHVCLDVRIALQHDICALEHALHIPLRDLATSDGMAKLRTAARADSPSPLPVYTLCRRGIASRRALVLLDDADFAAPVYNIDGGLKAWKRDVL"
  },
  {
    "reference": "MVQAIKLNDLKNRKRKNVEEENGSDESEIDISSTDSENEEEQNGEEEIVNIDFDFFGGNPEVDFHALKNLLRQLFGPQESTRIQLSSLADLILGSPTTTIKTDGKESDPYCFLSFVDFKANHLSDYVKYLQKVDMRLSTFFKTMIDSGNKNCALVLSERLINMPPEVVPPLYKITLEDVATALGDDKHYDFYIIVTRKYEVNFDTDDDTDSGKRNKNKDERSKKRVKADEVDYFHEEDRFFEKYAKIHFESEAKKGVISSYMILDHEGLVKSIDELETEISTW",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should prioritize the formation of turn-strand-strand-turn-helix-helix-helix-strand-strand-helix-helix-helix-turn-strand-helix elements.2. The protein should be designed to localize specifically to cytoplasm, nucleus in order to perform its role in protein export from nucleus, ribosomal large subunit export from nucleus more effectively.3. The protein must exhibit the following characteristics: Plays a role in actin cytoskeleton organization and vesicular transport.; May also play a role in directing MSS4 to the plasma membrane.4. Target a Acidic residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "TLPQVLSHYLQIYPEVPENNSANDDSDPLMHANNFKNMNAILFDELSKERTGAYHGSNLELYTPKSAIYHLKFDINIPYPLNIIISRTCMIKYQIILRYQLVLQYHSRLLDETWMDLNKTPSWKYRGYSHTVKRRIVRATRVLHAKMNHFIKTIMEYFNQNVIDKEVYSLEKCYRNPTLAVAIQNELEGGLTNIMTNRCLSDLIPLQLQIFDIVYKFCKFIKSMRS",
    "response#2": "TLPQVLSHYLQIYPEVPENNSANDDSDPLMHANNFKNMNAILFDELSKERTGAYHGSNLELYTPKSAIYHLKFDINIPYPLNIIISRTCMIKYQIILRYQLVLQYHSRLLDETWMDLNKTPSWKYRGYSHTVKRRIVRATRVLHAKMNHFIKTIMEYFNQNVIDKEVYSLEKCYRNPTLAVAIQNELEGGLTNIMTNRCLSDLIPLQLQIFDIVYKFCKFIKSMRL",
    "response#3": "TLPQVLSHYLQIYPEVPENNSANDDSDPLMHANNFKNMNAILFDELSKERTGAYHGSNLELYTPKSAIYHLKFDINIPYPLNIIISRTCMIKYQIILRYQLVLQYHSRLLDETWMDLNKTPSWKYRGYSHTVKRRIVRATRVLHAKMNHFIKTIMEYFNQNVIDKEVYSLEKCYRNPTLAVAIQNELEGGLTNIMTNRCLSDLIPLQLQIFDIVYKFCKFIKSMRL"
  },
  {
    "reference": "MAPGMSGRRGAALLCLSVLLAHAASRSHPASPSPPGTQASPILPVSYRLSHTRLAFFLKEARPLTPAAINGSLQRSEPFVVFQTKELPVLNVSLGPFSTSQVVARELLQPSSTLDIPERLTVNWKVRAFIVRARVPASQPVAQVLFYVAGRDWDDFGVTERLPCVRLHAFRDAREVRSSCRLGGALATCLVRAELPLAWFGPPAPAAPPSARRKSPDGLEPEAAAESQQVELYYTLHAPDASGGCGSARRGPGPGPGAAARAESPTQHPLLRIGSISLFRPPPRRAVQEHRLDSNLMIRLPDRPLRPGEVLSILLYLAPNSSSAASPSVEHFTLRVKAKKGVTLLGTKSRSGQWRVTSELLTGAKHSTATVDVAWALDTPLPPWEGQGPLEILQLDFEMENFTSQSVKRRIMWHIDYRGHSALPDLERAVTELTVIQRDVQAILPLAMDTEIINTAILTGRTVAIPVKVIAIEVTGLVLDVSDLVECQSHSEDIIKVSSSCDYVFVSGKESRGSMNARVTFRYDVLSAPLEMTVWVPKLPLHIELSDARLSQVKGWRVPILPDRRSARESEDEEEEEEERRQSANRGCTLQYQHATLQVFTQFHTTSSEGTDQVVTMLGPDWLVEVTDLVSDFMRVGDPRVAHLVDSNTLAGLEPGTTPFKVVSPLTEAVLGETLLTVTEEKVSITQLQAQVVASLTLSLRPSPGSSHTILATTAAQPTLSLVKQEALLSLWLSYSDGTTAPLSLYSSRDYGLLVSSLDERVATVTQD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Include a signal peptide as an essential component of the protein.2. The designed protein's functionality will benefit from Acidic residues, Polar residues compositional bias in its structure.3. The protein should have a Helical domain that is long enough to span the entire membrane.4. The designed protein should contain Cytoplasmic topological domains.5. The protein should be designed to localize specifically to cell body in order to perform its role in posterior lateral line neuromast hair cell development more effectively. The designed protein sequence is ",
    "response#1": "GNFELEILEISNTNSHLLNGYCCGMPAELRATKTIGCSPCTTAFRLCLKEYQTTEQGASISTGCSFGNATTKILGGSSFVLSDPGVGAIVLPFTFRWTKSFTLILQALDMYNTSYPDAERLIEETSYSGVILPSPEWKTLDHIGRNARITYRVRC",
    "response#2": "RPLTYQASLPELTDPVHVNPISVTPPIQAIDQDRNIQPPSDRPGILYSILVGTPEDYPQYFHMNLTTAELTLLKPINRDLHQKFDLVIKAEQDNGHPLPAFANLHIEVLDENNQKPYC",
    "response#3": "YCGVQTITMKINFCTVLFSGYSESDLSLNGKHGDAHCRGFINNNTFPAVVIFTINLSTLESCENSLVVSTVPGVNAYGIASMVQIGNISGYIDTPDPPTIISYLPGLLYKFSCSYPLEYLVNNTQLASSSAAISVREGNGTFISTLNLLLYNDSTYSQQLLIPSAGLPLKTKIYAAVRATNLDGRWNVLMDYCYTTPSGNPSDDIRYDLFLSCDKDPQTTIFENGKSQMGRFSFEVFRFVKHKNQKMSTVFLHCITKLCRSDDCHYLTPTCHNC"
  },
  {
    "reference": "MTQVSKTIAKPSGNANLYSRLFSYYRVYKKLIFIALAGLCLFSFVDAGMIYFVKPLIDQGLSKADSHTLQLGALLVVAIFFLRGIASFTSSYAIAYISSKVTYRIRQQAFDKLLYLPRTYFDLNSRGSLISKIIYDTEQLSQSFSSAVVIAIRESVIILVLFSMMVYNSWQLTAIFLVIVPIIALIINKVSKRFKNISHKLQNSMGQVSNKTEQAILNQQEIVLLDTRTQISAQFEKTNNNNRQQNMKLQATSAISNPVIQLIASFAIAAVLLLASIDQVLNQLTPGSFTLILIAMGSLLKPLKQLSNINQQLQKGLIAAKSLFSFLDQQEEHDIGTKQLSKTCSNIRFNNFSFTYQGKTQPALSNFSLQIKGGTSVAFVGESGSGKSTLARLLLRLYQSPKQSILINDIAIEDYSLSSLRAQFAFVSQDIVLIDDTLANNISFGCNRDVTDSEIEQAAINANVMAFAKELPLGLNSEIGENGRNLSGGQRQRIAIARAMLRDASIIVLDEATSALDNHSEKHIQQALTRLTQHKTVLIIAHKLSSIQHVDEIIVINKGRLIEQGNHKTLQAKAGYYQSLYQSQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must have at least one ABC transporter domain. The designed protein sequence is ",
    "response#1": "KSSGSTFDILHDVTLFNRDAEMLLVLGRPGAGCSTLLRVISNQRSSYVSVSGDVTYGGINSDEWKNFKGESIYTPEEDTHHPTLTVRETLNFALKCKTIHNRLPDEKKKTFRKKIYDLLVGMFGISKQSDTLVGNEFIRGLSGGERKRLTITEAMVSSASITCYDCSTRGLDAASALDYAKSIRIMSDTLHKTTIASFYQASDSIFNLFNNVAILEKGRLIYFV",
    "response#2": "KSSGSTFDILHDVTLFNRDAEMLLVLGRPGAGCSTLLRVISNQRSSYVSVSGDVTYGGINSDEWKNFKGESIYTPEEDTHHPTLTVRETLNFALKCKTIHNRLPDEKKKTFRKKIYDLLVGMFGISKQSDTLVGNEFIRGLSGGERKRLTITEAMVSSASITCYDCSTRGLDAASALDYAKSIRIMSDTLHKTTIASFYQASDSIFNLFNNVAILEKGRLIYFR",
    "response#3": "KSSGSTFDILHDVTLFNRDAEMLLVLGRPGAGCSTLLRVISNQRSSYVSVSGDVTYGGINSDEWKNFKGESIYTPEEDTHHPTLTVRETLNFALKCKTIHNRLPDEKKKTFRKKIYDLLVGMFGISKQSDTLVGNEFIRGLSGGERKRLTITEAMVSSASITCYDCSTRGLDAASALDYAKSIRIMSDTLHKTTIASFYQASDSIFNLFNNVAILEKGRLIYFY"
  },
  {
    "reference": "MSQFDQVSVLKKANVYFDGKCVSHTVLLADGTRKSVGVILPATLFFGTAAPEIMELVEGRCRVRLKGETAWTDYAGGQSFSVPGDSGFDIEALEPVHYVCHFG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Also catalyzes the reverse reactions. The designed protein sequence is ",
    "response#1": "PISPVQGFFLDLGLAGQIGLINYIFEVEGSDLKHGLAHVTNLLESKNPVFRTLIEKTKESGFVQILVAKTRSSWFYPSDLRLYLEKTMTQKFELGKPAVQYALVMEDAAHEGRNFFVISMHHTHCDAFSRFLIGKEISQILESPSYYARSENIERPWFGNYVKHVQQKATDDKASLFWDAYMCGANLANIYPLHRATLNGEFDGAIIEKIQAPVATRIADGSPRNSTQVILAAWAIALANLSGLRDITFGLCRHGRSSSSFRDVRRLMGPLVNVLPFRVSLICNEEPAPALLQRIQNEITSTNKHEHGQ",
    "response#2": "XGRPLLGCTIKPKLGLSAKNYGRACYECLRGGLDFTKDDENVNSQPFMRWRDRFVFCAEAIYKSQAETGEIKGHYLNATAGTCEEXXXXXXFARELGVPIVMHDYLTGGFTANTTLAHYCRDNGLXXXXXXXXXXXXXXXXXXXXXFRVLAKALRMSGGDHIHSGTVVGKLEGEREMTLGFVDLLRDDFIEKDRARGIFFTQDWVSMPGVIPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAAANRVALEACVQARNEGRDLAREGNEIIRAACKWSPELAAACEVW",
    "response#3": "PISPVQGFFLDLGLAGQIGLINYIFEVEGSDLKHGLAHVTNLLESKNPVFRTLIEKTKESGFVQILVAKTRSSWFYPSDLRLYLEKTMTQKFELGKPAVQYALVMEDAAHEGRNFFVISMHHTHCDAFSRFLIGKEISQILESPSYYARSENIERPWFGNYVKHVQQKATDDKASLFWDAYMCGANLANIYPLHRATLNGEFDGAIIEKIQAPVATRIADGSPRNSTQVILAAWAIALANLSGLRDITFGLCRHGRSSSSFRDVRRLMGPLVNVLPFRVSLICNEEPAPALLQRIQNEITSTNKHEHGC"
  },
  {
    "reference": "MLAAGEGTRMKSRRTKVLHEVAGRPLVAHVLASAAEAGTDAVAIVVGRDAEAVAALGRGMVDGVQCFEQTERLGTAHAVVAARAAIAEGYDDVLVLFGDTPLLSAATLSEARRQLADGAAVCVVGFEAADPAGYGRLLMDGAELLAIREDKDATPEERRVRLCNSGVMALDGRRALSLLDRVGNANAKAEFYLTDVVEIARADGLGVRVVLADEAETLGVNTRGDLALVEHLWQERRRAEMMAAGVTLIAPQTVFFSADTAIEADVVIEPNVVFGPGVSVGSGTVIHAFSHVEGARIGADVAIGPYARLRPGTDIEPKARVGNFVEIKNTKLGTGAKVNHLTYLGDASVGPRSNIGAGTITCNYDGYNKYRTSIGADAFVGSNSSLVAPVVIGDGAYVGSGSVVTEDVPDEALAVGRARQVTKPGRGAEINARNAARKAGRT",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc)2. The protein design should take into account the physiological conditions of the Nucleotide-sugar biosynthesis pathway.3. The designed protein should have a high specificity for UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA binding, with minimal off-target effects.4. The presence of Mg(2+) is necessary for the protein to function in the desired environment.5. The designed protein should be involved in regulation of cell shape, cell morphogenesis by localizing to cytoplasm and exhibiting glucosamine-1-phosphate N-acetyltransferase activity. The designed protein sequence is ",
    "response#1": "VLILAAGLGTRMRSNLAKVLHKLDGRPLINHVCRTAMALAPEKIYVVVGHQAEDVKTAVLNELDESIVEFVIQEKQLGTGDAVNAARNHLQAQDSTLLILSGDVPMIRAETLAKLVQQHRDHRGKGAAATLTLSAIVPDATGYGRIVRDASGEAVAIVEQKDASPEQLAVNEVNTGILAAPTQLLKRWLASLSNDNAQGEYYLTDIVAQAVADGVPVVSAQPAHAWETDGVNSKAQLARLERWHQQQVADQLLEQGVALIDPSRLDVRGSLTCGHDVEIDVGCIFEGEVELGEGVRIGPYCVIRDSHIGAESVVEAHSVIEGTVAAGLNRIGPFARLRPGSRLAVRAKVGNFVETKNVEVGEGSKINHLSYVGDASLGRNVNVGAGTITCNYDGVNKHRTEIGDEAFIGSNTALVAPVTVGRGATVGAGSTISKDIADGALAVARGRQRNIQGWKRPTKRK",
    "response#2": "VVILAAGKGTRMRSDLPKVLHSVAHKPMVQHVIDTARELNADNINLVYGYGGELLKAKLGEQPLNFVLQAEQLGTGHAVAQAIDHINDEDTVLVLYGDVPLTRKETLEALLAARQPDGVAVLTTRVDDPTGYGRIVREDGVVTAIVEHKDATAEQRAITEINSGIYAFDAAVLRDALKRVTTDNAQGEKYLTDVLGIARSDGVPIGAGVAADHWLTEGVNDRVQLATLGAELNRRTVEAHQRATVIEDNVFVGSDTQLVAPVVIRKGATLGAGSTVTGAGVTIVDPATTWIDSAVTIAPDVTLLPGTQLLGATVIGSGSTIGPDTTLRNVEVGRDATVVRTHGSDAVLGDGTTVGPYAYLRPGAVLGTGGKIGTFVEVKNSTIGEGAKVPHLSYVGDADIGDGANIGAGTIFANYDGVNKHRSTIGRHAKTSSNNTFVAPVSVGDGAFTGAGATIREDVPAGALAVSAGGATVRRGARVVDA",
    "response#3": "IILGAGMGTRMNSDLPKVLHQIAGAPMLVHAMKSGGALNPDHTIIVAGHGAVAVEKAAKAYDADAMIALQAEQLGTAHAVAQARSALDGFDGDAIVLYADTPFISPETLAAMSAARLTHDIVVLGFEAKDPARYGRLIADGERLERIVEYKDANDEERSITFCNSGVICADAKTLFDLISAVGNDNASGEYYLTDIVELARRDGLSATAIVCDEAETMGVNSRSQLAAAEAAFQSRARA"
  },
  {
    "reference": "MVAVEFKVSESGRPRPEKNPKLGFFVYLLGSAAIIGGFLFGYDTSVVSAAMLYVPEAPGLKPMGTVWKEVIVSITPGMAAVGAWFSGAGSDRYGRKPIIIGSTLIFVCGAVICAVAWTKIVMLIGRIFLGVGIGFASMVVPVYLGEASPTHVRGTLVSAFAMMISFGQVVANIMGGVFSYWEPYTIGWRLMFAFAGIPALIQFVCFIFLPETPRWLYENGHTEQAEQVLEKIYGGNTEWIEYELAEIKTYAEERQKQMEEEKKSGPVIWRILKTPHVLKACFIGSMLQAFQQLAGINTILYYTADIIRSAGIENYHTIIWISVILSICNLIGPFAPMFFIEKLGRRKLFLFSCAGVVVSLVLIGVSFLLVGNDSAPNFDRSAYLLAGNYQSNGEAESCLMLSNCDSCVTSEHCGFCEDSETRTGFCLPVDHNDVTLYSSTGLCTNGLDKSNSSFPNATSYVWQKHHCTTSYTILPIVMMGVYLLTFSCGFTSLPWVLNSEFYPMWARSTCVSISTLSNWVFNLIIALTYLSLTHAITKYGAFWLYAIFTIIAFIFIYFLVPETTGYSIDEVEMLFMNKRQRNIAMQARQAKLDAASDKDKNSSTSLSTETITM",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should aim to mimic or enhance the function of natural proteins involved in transmembrane transport, potentially with improved properties such as stability, specificity or selectivity.2. The protein should have a Helical; Name=5, Helical; Name=9 domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "GNGLVIWIFCAAKSLRTPSNMFVVNLAICDFFMMIKTPIFIYNSFNTGFALGNLGCQIFAVIGSLTGIGAAITNAAIAYDRYSTIARPLDGKLSRGQVILFIVLIWTYTIPWALMPVMGVWGRFVPEGFLTSCSFDYLTDTNEIRIFVATIFTFSYCIPMILIIYYYSQIVSHVVNHEKALREQAKKMNVDSLRSNANTSSQSAEIRIAKAAITICFLYVLSWTPYGVMSMIGAFGNKALLTPGVTMIPACTCKAVACLDPYVYACLNHDFRRAFKKILCR",
    "response#2": "GNGLVIWIFCAAKSLRTPSNMFVVNLAICDFFMMIKTPIFIYNSFNTGFALGNLGCQIFAVIGSLTGIGAAITNAAIAYDRYSTIARPLDGKLSRGQVILFIVLIWTYTIPWALMPVMGVWGRFVPEGFLTSCSFDYLTDTNEIRIFVATIFTFSYCIPMILIIYYYSQIVSHVVNHEKALREQAKKMNVDSLRSNANTSSQSAEIRIAKAAITICFLYVLSWTPYGVMSMIGAFGNKALLTPGVTMIPACTCKAVACLDPYVY",
    "response#3": "GNGLVIWIFCAAKSLRTPSNMFVVNLAICDFFMMIKTPIFIYNSFNTGFALGNLGCQIFAVIGSLTGIGAAITNAAIAYDRYSTIARPLDGKLSRGQVILFIVLIWTYTIPWALMPVMGVWGRFVPEGFLTSCSFDYLTDTNEIRIFVATIFTFSYCIPMILIIYYYSQIVSHVVNHEKALREQAKKMNVDSLRSNANTSSQSAEIRIAKAAITICFLYVLSWTPYGVMSMIGAFGNKALLTPGVTMIPACTCKAVACLDPYVYAFGSPQVRSAARR"
  },
  {
    "reference": "MSSLQIILLLIIAAITGIASVLDEGQTHRPLVACTLVGLVLGDLKTGIILGGTLELMALGWMNVGLAMAPDTAIASVISTILVITADQGIGEGIAVAVALAAAGQALTIFVRTITVFFIHRADQYAKAGNIKGIEIMHITAMVFQALRVMIPTLIVALISVSAVQAFLGNIPDVITKGLQIGGGIIVVVGYAMVINMMNIPYLKPFFYIGFLLAAFTDFNLVGFGALGLCLALLYQQVMQKQSAHGAVAAASDSGSVAVYDDDDDDLDA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be able to regulate phosphoenolpyruvate-dependent sugar phosphotransferase system.2. For general function, the protein need meet that This system is involved in fructose transport.; The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane3. The designed protein should have at least Helical transmembrane domains to ensure proper localization.4. The presence of PTS EIIC type-4 domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "DPIFAAGKLGPGIAIQPTGNTVVAPADATVILVQKSGHAVALRLDSGVEILVHVGLDTVQLGGEGFTVHVERRQQVKAGDPLITFDADFIRSKDLPLITPVVVSD",
    "response#2": "GSFRQWVGKLSNGLMIPIAVLPIAGIFLGVGDAIAGNAGDLTGLRYFGLFIKNGGDVVFANLPILFAIAIAITFSQDAGVAGFSAFVFWAAMNGFMSSLILPFDKAGKIITDTSTPIAGFKVLYNKSVPVHAIATTLGLRTLSTSVFGGIIVGALTSVLYKKFYAIRLPDVIGFFSGTRFVPIICFVVAIPVALILLMIWPAVSIGLNAIGTGLGFLGGKGYGANSLIFGYIERSLIPFGVHHAFYAPLWYTSAGGSLQEIVNQQVWIRPDFHLSDNYVARVIGWVDPNNSSMYIIPGALNGQNGSSTGNTMSKDLNGALSAYMSKESTAFLTWKDLVDGLTFKGNFDKMAENGLLDGSNKIWLGLNGSGILGKKLLLSDGNVYTITFKTFANTTPIAWSKGAQAVLPLNASSTIVNNPTALAAATQSNNNTNNIKLYPVNSFRVAVESLNPAQYSQGKFPFMLFGIPAAGVAMILAAPKDRRKEAASIVGSAAFTSFLTGITEPFEFTFLFLAPWLFYGVHAVLAAVSFWLMNILGANVGQTFSGSFIDFILYGALPDGRRWLANSYLVPIIGLFLAAIYFPTFYFLIKHFNLATPGRGEFLSDTFRPILWALLGASLIITLLVLADTFGLQDFRAPMDEQPDTYVFLHSMWRSVFYFLPIMVGATAARKLGANEWIGAAIPAALLTPEFLALGSAGDTVTVFGLPMVLNDYSGQVFPPLIAAIGLYWVEKGLKKIIPEAVQMVFVPFFSLLIMIPATAFLLGPFGIGVGNGISNLLEAINNFSPFILSIVIPLLYPFLVPLGLHWPLNAIMIQNINTLGYDFIQGPMGAWNFACFGLVTGVFLLSIKERNKAMRQVSLGGMLAGLLGGISEPSLYGVLLRFKKTYFRLLPGCLAGGIVMGIFDIKAYAFVFTSLLTIPAMDPWLGYTIGIAVAFFVSMFLVLALDYRSNEERDEARA",
    "response#3": "SDTFRPILWALLGASLIITLLVLADTFGLQDFRAPMDEQPDTYVFLHSMWRSVFYFLPIMVGATAARKLGANEWIGAAIPAALLTPEFLALGSAGDTVTVFGLPMVLNDYSGQVFPPLIAAIGLYWVEKGLKKIIPEAVQMVFVPFFSLLIMIPATAFLLGPFGIGVGNGISNLLEAINNFSPFILSIVIPLLYPFLVPLGLHWPLNAIMIQNINTLGYDFIQGPMGAWNFACFGLVTGVFLLSIKERNKAMRQVSLGGMLAGLLGGISEPSLYGVLLRFKKTYR"
  },
  {
    "reference": "MGYKIAVVGATGNVGREMLNVLVEREFPYDEIAAVASSRSQGSEIEIGDTGKMLKVQNIEHFDFSGWDIALFAAGSGPTKEYAPKAAAAGCVVIDNSSLYRMDPDVPLIVPEVNPEAISGYTKRNIIANPNCSTAQMVVALKPLHDAATIKRVVVSTYQSVSGAGKGGMDELFEQSRAIFVGDPKESHVFTKQIAFNVIPHIDVFLDDGSTKEEWKMVVETKKILDPKIKVTATCVRVPVFVGHSESINIEFEKELSAKDAQKILRDAPGIMLVDKREDEGYVTPVECVGDGATFISRVREDPTVENGLNIWCVSDNLRKGAALNAVQIAELLGRKHLKKG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.2. The NADP(+), phosphate, substrate binding site should be located in a region of the protein that is accessible to the ligand.3. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.4. The presence of Semialdehyde dehydrogenase NAD-binding domains is critical for the interaction of the designed protein with its intended targets.5. The designed protein should have NAD binding, protein dimerization activity, NADP binding to facilitate lysine biosynthetic process via diaminopimelate, isoleucine biosynthetic process, diaminopimelate biosynthetic process, 'de novo' L-methionine biosynthetic process, threonine biosynthetic process.6. Ideal protein should have a binding affinity for key enzymes in the Amino-acid biosynthesis, L-threonine biosynthesis, L-threonine from L-aspartate: step 2/5 pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MTRVAILGATGAVGTELLELLDSRNFPVAELKLLASSRSAGTQLRFKEEKIAVEAVNDQSFDQVDLVLASAGATTSKQWAARAVEAGAVVIDNSSAFRMDAQVPLIVPEVNPEAAASRPRGIIANPNCTTMMMLAVVGATGQVGVAMRSILEQRDFPADRVRFFASARSAGKTLPFRGEEIVVEDAATADVSGIDVALFSAGATTSRVQVPRFAEAGAMVIDNSSAFRKDPEIPLIVSEVNPEDIQKGRRGPNIIANPNCTTMQMVVALKPLHDEAGLQRLIVSSYQAVSGSGLAGVEELATQARAVIGDAEKLTHDGSAVPFPAPNKYVAPIAFNVLPLAGSIVDDGSGETDEDQKLRNESRKILGLPDLLVSGTCVRVPVFTGHSLSINAEFARPLSVERAKELLADAAGVKLVDVPTPLAAAGKDESLVGRIRQDPGVPEGRGLALFVSGDNLRKGAALNAVQIAEVVVE",
    "response#2": "MTNIGVVGATGQVGAVVRRLLAERDFPVASIRFFASARSAGTYLPFKGVDVIVEDAAIADPTGLDVAIFSAGATLSKAQAPRFAAAGVTVVDNSSGWRMDPDVPLVVSEVNPEAIDLLKPLHDIVPIRRVVVSTYQSVSGAGMGAMEELEAQVRGELFNEQAAIERLPRRIAFNVVPHIDVFEDDGSTREEWKMVHETRKILDPEIALAATCVRVPVFIGHAEAVNVEFAGPVSPSDATDALMVAPGVEVVDADDVQMYATPIDCVHEDPVFVSRIRTDPTVDHGLQFWTVADNLKPLHDIVPIRRVVVSTYQSVSGAGMGAMEELEAQVRGELFNEQAAIERLPRRIAFNVVPHIDVFEDDGSTREEWKMVHETRKILDPEIALAATCVRVPVFIGHAEAVNVEFAGPVSPSDATDALMVAPGVEVVDADDVQMYATPIDCVHEDPVFVSRIRTDPTVDHGLQFWTVADNLKPLHDIVPIRRVVVSTYQSVSGAGMGAMEELEAQVRGELFNEQAAIERLPRRIAFNVVPHIDVFEDDGSTREEWKMVHETRKILDPEIALAATCVRVPVFIGHAEAVNVEFAGPVSPSDATDALMVAPGVEVVDADDVQMYATPIDCVHEDPVFVSRIRTDPTVDHGLQFWTVADNLKPLHDIVPIRRVVVSTYQSVSGAGMGAMEELEAQVRGELFNEQAAIERLPRRIAFNVVPHIDVFEDDGSTREEWKMVHETRKILDPEIALAATCVRVPVFIGHAEAVNVEFAGPVSPSDATDALMVAPGVEVVDADDVQMYATPIDCVHEDPVFVSRIRTDPTVDHGLQFWTVADN",
    "response#3": "MKIAVVGVTGMVGQVMLQVLEERNLNVSTLIPVASEKSVGTEITFKGKQIKVVGMKQAIELKPDIALFSAGGSVSLEFAPQFAAVGTTVIDNSSAFRMDSDKKLVVPEINAGSQRLVSGTCVRVPVFTGHLKPLHDIVPIRRVVVSTYQSVSGAGMGAMEELEAQVRGELFNEQAAIERLPRRIAFNVVPHIDVFEDDGSTREEWKMVHETRKILDPEIALAATCVRVPVFIGHAEAVNVEFAGPVSPSDATDALMVAPGVEVVDADDVQMYATPIDCVHEDPVFVSRIRTDPTVDHGLQFWTVADNLKPLHDIVPIRRVVVSTYQSVSGAGMGAMEELEAQVRGELFNEQAAIERLPRRIAFNVVPHIDVFEDDGSTREEWKMVHETRKILDPEIALAATCVRVPVFIGHAEAVNVEFAGPVSPSDATDALMVAPGVEVVDADDVQMYATPIDCVHEDPVFVSRIRTDPTVDHGLQFWTVADNLKPLHDIVPIRRVVVSTYQSVSGAGMGAMEELEAQVRGELFNEQAAIERLPRRIAFNVVPHIDVFEDDGSTREEWKMVHETRKILDPEIALAATCVRVPVFIGHAEAVNVEFAGPVSPSDATDALMVAPGVEVVDADDVQMYATPIDCVHEDPVFVSRIRTDPTVDHGLQFWTVADNLKPLHDIVPIRRVVVSTYQSVSGAGMGAMEELEAQVRGELFNEQAAIERLPRRIAFNVVPHIDVFEDDGSTREEWKMVHETRKILDPEIALAATCVRVPVFIGHAEAVNVEFAGPVSPSDATDALMVAPGVEVVDADDVQMYATPIDCVHEDPVFVSRIRTDPTVDHGLQFWTVADN"
  },
  {
    "reference": "MLRHDSRSENQLRNVEIETNVMKYSEGSVLITFGDTKVLCSATVEERVPKFIRGKGTGWIAAEYQMLPRATQTRNMRESTKGKVSGRTMEIQRLIGRSLRAVIDLTLLGERSIIVDCDVLQADGGTRTASITGAFVAVQLAIQELMRKNIIKKNPVKDYLAAISVGILPSGECVLDLDYKEDVAALVDMNLVMTGSGRFVEIQGTGEEATFSGEELNTLLFYGKSGIETLIAKQKEVLLVSKRQETSQEKKEVLIATRNKGKAKEFAAIFAEKGFTVKTLLDYPDLPDVEETGKTFEENARLKAETIAQIVQGPVLADDSGLIVDALLGQPGIYSARFAGPNKSDAANNAKLLHELTGIATEKRTARYHCTLVLAEPNKESLVVSGELEGTIGTIPQGENGFGYDSLFYIPSEKKTAAELSEDQKNRISHRAKAIEALRGQWKS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein requires Mg(2+) to activate a critical catalytic site.2. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for phosphate + tRNA(n+1) = a ribonucleoside 5'-diphosphate +   tRNA(n).3. The designed protein should have ribonucleoside triphosphate phosphatase activity, dITP diphosphatase activity, XTP diphosphatase activity, tRNA binding, nucleotide binding to facilitate nucleotide metabolic process, tRNA processing, rRNA catabolic process, rRNA processing.4. The protein should have a high affinity for phosphate, Mg(2+), substrate.5. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.6. The designed protein must have at least one Exoribonuclease phosphorolytic domain. The designed protein sequence is ",
    "response#1": "MEIRDIKITRNYTRYAEGSVLIEMGETKVICTASIEDKVPPFLRNSGTGWINAEYSMLPRSTHQRKIRESSRGKIDGRTQEIQRLIGRAIRSVVSLDKIGERTIWVDCDVIQADGGTRTASITGAFVAVADAVNKLADAGKIKADFVAAVSVGVLPTKEVVLDLDYVEDSAAAVDMNLVMTESGEFVEIQGTGEEATFTRQELAAMLEVGEEGCRKVIAIHLEKEKGEDKIELDDIPLTIPISKLLSK",
    "response#2": "MEIRNLNFQINYTPNPDGSVLVECGNTKIICTAMIEDKVPFFLKGSNSGWLSCEYSMLPSSTQSRKVRDISKGKIDGRSQEIQRLIGRSLRSCIDLKLLGEKTIWIDCDVISADGGTRTASINGAFVALKLAIHSSMKKIVGIKCNRIHMDFIAAISVGQIEKEIFLDLCFEEDSKADVDMNIVMTGKGELVEVQGTAEKKPFSREELNKFLDLKIDFIAAISVGQIEKEIFLDLCFEEDSKADVDMNIVMTGKGELVEVQGTAEKKPFSREELNKFLDLLNDVDFIAAISVGQVEGNILLDLSYEEDSKADVDMNIVMTGRGELVEIQGTAEKKPFGRDQLDKFLELAEQGIEKLILLKEKE",
    "response#3": "MSEIRNLNFQINYTPNPDGSVLVECGNTKIICTAMIEDKVPFFLKGSNSGWLSCEYSMLPSSTQSRKVRDISKGKIDGRSQEIQRLIGRSLRSCIDLKLLGEKTIWIDCDVISADGGTRTASINGAFVALKLAAEESDKLKKIAVSDFIAAISVGQIEKEIFLDLCFEEDSKADVDMNIVMTGKGELVEVQGTAEKKPFSREELNKFLDLSGKFISAVSVGIVNGENILDLCYEEDSNAQVDMNIVMTDKGEFVEVQGTGEEFPFKKDQLYKLLDIGSRIRIYEGKGSASDFIAAISVGQIEKEIFLDLCFEEDSKADVDMNIVMTGKGELVEVQGTAEKKPFSREELNKFLDLGEFVSAISVGIVNKTHMLDLCYEEDSSAQVDMNVIMTDSGEFVEVQGTGEERPFSRDDLNELLKLGKKAEVIRYED"
  },
  {
    "reference": "MAENSVLTVSIVTPDGQVYDDDQVTMLVVNTKEGELGILPNHVPVIAALAIDEVRVKHSQEEDVIAVNGGFVEFSENTATIVADTAENQSDIDVARAESAKKRAEATIRKAQQAHDNSELRRAQISLRRAINRINVSKH",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein can be expressed in proton-transporting ATP synthase complex, catalytic core F(1), plasma membrane and perform ATP binding without triggering undesirable cellular responses.2. For general function, the protein need meet that Produces ATP from ADP in the presence of a proton gradient across the membrane. The designed protein sequence is ",
    "response#1": "GTRLVDTLVPIGKGQRQLIIGDEGTGRSSLAIDAVLNQKGREVRCVYVLIGQKRSTVVGTIELLRNYGALAYTTVVVGEAGSLPGLKYLAPFAGCAIAEDWMARGHDTLVVYDDLSTHAQTYRELSLLLRRPPGREAYPGDIFYLHARLLERATALNPANGGGSMTALPIVETRLGEIEAYIPTNLISITDGQIYLDSDLFAAGFKPAIDVTRSVT",
    "response#2": "GTRLVDTLVPIGKGQRQLIIGDEGTGRSSLAIDAVLNQKGREVRCVYVLIGQKRSTVVGTIELLRNYGALAYTTVVVGEAGSLPGLKYLAPFAGCAIAEDWMARGHDTLVVYDDLSTHAQTYRELSLLLRRPPGREAYPGDIFYLHARLLERATALNPANGGGSMTALPIVETRLGEIEAYIPTNLISITDGQIYLDSDLFAAGFKPAIDVTRSVS",
    "response#3": "GTRLVDTLVPIGKGQRQLIIGDEGTGRSSLAIDAVLNQKGREVRCVYVLIGQKRSTVVGTIELLRNYGALAYTTVVVGEAGSLPGLKYLAPFAGCAIAEDWMARGHDTLVVYDDLSTHAQTYRELSLLLRRPPGREAYPGDIFYLHARLLERATALNPANGGGSMTALPIVETRLGEIEAYIPTNLISITDGQIYLDSDLFAAGFKPAIDVTRSVT"
  },
  {
    "reference": "MASPLPLVFDAPKRGLPPRHFADLDPADRRAAVTELGLPGFRADQIARQYYARLESEPDAMTDLPAGVRSAIAEKLFPPLLQEIRNVACDDGETRKTLWRAHDGSLLESVLMRYPDRATLCISSQAGCGMACPFCATGQAGLQRNLSTAEIVDQVRSAARAMRDGEIHGGPGRLSNIVFMGMGEPFANYKRVVAAVRRITSAPPDGFGISHRNVTVSTVGLAPAIRKLADEKLGVTLAVSLHTPDDELRDTLVPVNNRWSVREVLDAARYYADSTGRRVSIEYALIRDINDQPWRADMLGTKLRKALGQYAHVNLIPLNPTPGSKWDASPKDVEREFVRRVRAQGVSCTVRDTRGQEIAAACGQLAAES",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. We need a protein that can perform adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine with high efficiency.2. The presence of Radical SAM core domains is critical for the interaction of the designed protein with its intended targets.3. The designed protein localizes to the cytoplasm.4. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.5. The protein should be able to bind S-adenosyl-L-methionine ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "QPYGNTLCVSTQVGCAYGCFMCATGKMGLIRNLLPGEMTGQLLAIEKDKGVKVKNIVFMGMGEPLANYDNLKKAIELFSDSDGIGVNQRRITVSTCGILPGIKKLIEDKLRVKLSISLHSTFDSVRDKLVPVNKKYNLAHLFETLREYCTSTMFQITIEYVLIEGINDGIGEVDNLITLIRKYNLGVKVKNIVFMGMGEPLANYDNLKKAIELFSDSDGIGVNQRRITVSTCGILPGIGKISQKKLKALGFYTGADLKTWSRKELLNTFGYFGEHLYQRVRGIDNSPVVAEKAFPYGNSVCVTTQVGCNIGCSFCASGLLRKSRDLTAGEIVGQIMKVQEHLDAKGEDERVSHIVVMGIGEPFDNYTHLMNFLHVVNDQKGLCIGARHITVSTSGLTEKIKDFANENLQVNLAVSLHAPNNELRSQIMKINKAFPIEKLMDSINYYLETTNRRITFEYILLRDVNDHVKEAEELGRLLADKRHLSYVNLIPYNPVDEHGQYQRSEPEAIKSFYEVLRRKGIHCGVRLENGTDINEE",
    "response#2": "GDGSRTVCVSTEAGCQIDCSFCATGHLGFKRVLSPSEIAEQVLSFSRELKAKGEKVTNVVFMGMGEPFLNYDNVKKAILLLNDKDGFNLGQRRISVSTSGIIPGIERFGAEDWQINLAISLHAPDNALRSRLMPINKTYPLEKLMPALDAYVAKNNRRLMIEYLLLGGINDSPRQADALADLIHAYGEILRLSVVNLIIYNPTKDSDGHWDRFRSPSLAALRAFEEILTKREIAWTEAPAMPERERYGEAAYRRKQIAQWIFEKRATDFAAMSNLPAALRTELAAAFRIRGHELVRVQGASDTTQKLLFKLSQGDFIECVVIPASPALYGERSDRHTLCVSTQVGCAYGCKFCASGLEGWKRNLTPGEIVGQILEAERHTGKRINNLVFMGMGEPMANFDNLMAAIDRINAPDGLNIGARHITVSTSGVVPRIRDLADQPRQIRLAISLHGASNEVRSQIMPVNRKHPVEELLAACAYFASKKKQKITFEYILIEGVNDSLDQARLLAAHARKLQAKVNLIPYNTVEGLDWHRPSVPVIEAFRDAVEHGGASVTLRLEKGHDIAAACGQLRLRQLKEG",
    "response#3": "QPYGNTLCVSTQVGCAYGCFMCATGKMGLIRNLLPGEMTGQLLAIEKDKGVKVKNIVFMGMGEPLANYDNLKKAIELFSDSDGIGVNQRRITVSTCGILPGIKKLIEDKLRVKLSISLHSTFDSVRDKLVPVNKKYNLAHLFETLREYCTSTMFQITIEYVLIEGINDGIGEVDNLITLIRKYNLGVKVKNIVFMGMGEPLANYDNLKKAIELFSDSDGIGVNQRRITVSTCGILPGIGRVSERRLERLGINTCRDLQTWSKPELLQQFGSFGRQLHQHARGLDDSIVNPEREVSSQAGCGMNCPFCATGQAGLTRNLSTGEIVDQVVAAARLLAEGGVGGSPVRLSNVVFMGMGEPLANYKAVMGALRRLTDKDSHGLGLSRRSITVSTVGLVPRIDQLTEENLPVTLAVSLHAPDDELRDTLVPVNTRWKVSEVLEAADRYSRSTGRRYSIEYALIKDINDQQNQAQQLDLF"
  },
  {
    "reference": "MEPFTTHTGRAVPLRRSNVDTDQIIPAVYLKRVSRTGFADGLFAAWRQDPGFVLNRPEFAGADILVAGPDFGTGSSREHAVWALQDYGFRVVISPRFGDIFRNNALKGGLLAVVLPESDVEALWDVVESDPATPVTVDLVTREVRYADVVRGFDIDDYTRWRLMEGLDDIGLTLRHADAIDAFEARRPAWKPTTRAS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate..2. The desired protein should be able to perform (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate3. The designed protein should interact with enzymes and substrates within the Amino-acid biosynthesis, L-leucine biosynthesis, L-leucine from 3-methyl-2-oxobutanoate: step 2/4 pathway to enhance or regulate its activity.4. The designed protein should have 3-isopropylmalate dehydratase activity within 3-isopropylmalate dehydratase complex to regulate leucine biosynthetic process. The designed protein sequence is ",
    "response#1": "MRRTDVDTDQIIPAVYLKRVTKTGFEDALFAGWRKDPGFVLNQEPYRPGSVLVAGQDFGTGSSREHAVWALRDYGFRAVLAPRFGDIFRGNAGKQGLLAAVVAQSDIDLIWKALEAHPGTEVTVDPFTAFRLVRERFAPELPVVVPGPEVAFADALRAMLVRADGRGTVSEPRPADLDALASALRSVAPSGRRGPAPLPLANIDTDQIIPKQFLKTVERAGLAKGLFYDLRFDEQGREKPNFVLNRPEYKGAGVLVAGDNFGCGSSREHAPWALMDFGIRCVISTSFADIFYGNCFQNGLLPVVLKAEEVQQLMDEARGGNHVVSVDPSWVPDERLAVLILAGDGIGAEVLAEVRRVAEWFRDQRGIRIDLREELFGLSAWQRHGTLMRDETWQEILASDAILFGAIGSPDYDKIPAEARKVDQLLRMRRELDLFTNLRPVRTFEALADTSTLRPEVIEGCDMMIVRELCGGIYFGTPRGIESLPDGSERAVNTTVYTTAEIQRIARSAFELARVRQGRLCSVDKANVLVETGGLWRKVVQELHDREYPDIELSHMYVDNCAMQIVRNPKQFDVLVTENLFGDIISDCAAMVAGSLGMLPSASIGPVRSDGRQQALYEPIHGSAPDIAGRGIANPLGAILSFALCLRYSLKQPQEAERLERAVEGAIVKGARTLDIAAVDKTPISTREMGDAVAAALDE",
    "response#2": "MRRTDVDTDQIIPAVYLKRVTKTGFEDALFAGWRKDPGFVLNQEPYRPGSVLVAGQDFGTGSSREHAVWALRDYGFRAVLAPRFGDIFRGNAGKQGLLAAVVAQSDIDLIWKALEAHPGTEVTVDPALASLVLRDHINTDEIIPARHLTTDIEAELAPYAMEDYDRDFAKRVQPGDIIVAGADFGCGSSREHAVWALRGAGVGAVIAPNFARIYYRNSINNGFLALECDGIVEAFQDGDEANLDALAAVSAQLATTDPERVLILAGDGIGAEVLAEVRRVAEWFRDQRGIRIDLREELFGLSAWQRHGTLMRDETWQEILASDAILFGAIGSPDYDKIPAEARKVDQLLRMRRELDLFTNLRPVRTFEALADTSTLRPEVIEGCDMMIVRELCGGIYFGTPRGIESLPDGSERAVNTTVYTTAEIQRIARSAFELARVRQGRLCSVDKANVLVETGGLWRKVVQELHDREYPDIELSHMYVDNCAMQIVRNPKQFDVLVTENLFGDIISDCAAMVAGSLGMLPSASIGPVRSDGRQQALYEPIHGSAPDIAGRGIANPLGAILSFALCLRYSLKQPQEAERLERAVEGAIVKGARTLDIAAVDKTPISTREMGDAVVAALRP",
    "response#3": "MRRTDVDTDQIIPAVYLKRVTKTGFEDALFAGWRKDPGFVLNQEPYRPGSVLVAGQDFGTGSSREHAVWALRDYGFRAVLAPRFGDIFRGNAGKQGLLAAVVAQSDIDLIWKALEAHPGTEVTVDPREAGRILAREAELFHPPASLRVVALDAANVDTDAIIPKQFLQKVSRLGFGKHLFHDWRFLDDAGEQPNPEFVMNHARYQGASILLARENFGCGSSREHAPWALADYGIRAMIAPSFADIFYGNSINNQMVPVRLTEQEVDELFQYVQATEGAQIEVDRFYGLEYANIDTDQIIPKQFLLAVSKQGFGKHLFHDLRYLDDKESVLNMDFNLNKKEYQNSSILVSFENFGSGSSREHAPWALVDYGIRAIIAPSFADIFKNNALGNGLLTIELAKDEVLEIVDELKKSQDKNIEISRLFGHWLEHPDNVPRVLILAGDGIGAEVLAEVRRVAEWFRDQRGIRIDLREELFGLSAWQRHGTLMRDETWQEILASDAILFGAIGSPDYDKIPAEARKVDQLLRMRRELDLFTNLRPVRTFEALADTSTLRPEVIEGCDMMIVRELCGGIYFGTPRGIESLPDGSERAVNTTVYTTAEIQRIARSAFELARVRQGRLCSVDKANVLVETGGLWRKVVQELHDREYPDIELSHMYVDNCAMQIVRNPKQFDVLVTENLFGDIISDCAAMVAGSLGMLPSASIGPVRSDGRQQALYEPIHGSAPDIAGRGIANPLGAILSFALCLRYSLKQPQEAERLERAVEGAIVKGARTLDIAAVDKTPISTREMGDA"
  },
  {
    "reference": "MKLVFASNNPNKIKEIQQLLPDSIQVLSLSDINCDADIPETADTIEGNAILKANYVTEHYGYNCFADDSGLEVDALNGAPGVISARYAGDHKNDNDNIDKLLSELETENNRKANFKTVIALNLNGTQQLFTGIIHGTIIRERKGTNGFGYDPVFVPENDSRTFAELEMEEKSGISHRGKAVKQLAAFLNQ",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must have high specificity towards ribonucleoside triphosphate phosphatase activity, nucleotide binding.2. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.3. The substrate binding site should be stable and able to withstand changes in environmental conditions.4. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MKKIVFATNNLNKLKEINNMLIDKYELLSLKDINCNIDIPETNDTIEGNAILKANYIKNNYGYDCFADDTGLEVKALDGAPGVYSARYSGSRDAVANMNKLLKNLENKTDRSAQFKTVIALNLNDQQYLFTGIVNGSILLEKRGNNGFGYDPIFIPDGYSKTFAELPMSEKNKISHRAIAMQKLVSFLNV",
    "response#2": "MKRLEKLVIATNNQGKLVEMRELLKPFGVEVVSQKELGIDEVEETGLTFVENAILKARHASKHSGLPAIADDSGLVVDALDGAPGVYTADWAETPNGRDFVMAMQKTHDLLVEHNVTEPARYRAQFVCVLVLMHPLDEPVPVIAQGVWHGQITHAPHGTNGFGYDPHFVLPSLNKTAAELTAEEKNAISHRGKALAQLVKTLQT",
    "response#3": "MRLVVATKNAGKTAEIREILSPLGFEIVSLGELGAPEEIEDAGTFAENAAKKARAVARATGLPALADDSGIEVDALGGAPGVYSARYAGEGATDEENNAKLVRELAGVPEAERGARFVCVLALVRAEGADPILVRGEWHGEILPAPRGTGGFGYDPLFVVPGTGRTTAELRPEEKNRISHRGRALAGLRGVLRRV"
  },
  {
    "reference": "MSAPARVLVVDDSATMRGLISAVLNADPDITVVGQAADALEARQAIKQLDPDVVTLDIEMPNMNGLEFLDKIMRLRPMPVIMVSTLTHRGAEATIAALEIGAFDCVGKPQPGDTHPFRDLADKVKAAARSQRKSMITSNRAAAPAATAVSDSRAGRKIVAIGASTGGVEALITVLQKFPANCPPTVITQHMPHTFTKSFAERLNRLCAPTVQEATDGARLEVGRVYLAPGGERHLEVHNAAAPCCRLVDHPPVNGHRPSVDVLFDSVAELAGRNAIGVILTGMGRDGAAGLLKLRHAGARTFGQNEKTCVVYGMPRVAYELGAVETQLPLGSIGEEILKTAAARKEGIE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. A protein with phosphorelay response regulator activity, protein-glutamine glutaminase activity, protein-glutamate methylesterase activity is needed to enhance protein demethylation, protein deamination, chemotaxis in a specific context.3. The protein must exhibit the following characteristics: Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR; Involved in chemotaxis; Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid.. The designed protein sequence is ",
    "response#1": "ALGASTGGVQALSRILPFFPAQSPGLLVVQHMPEGFTAAFARRLDALCRMRVREAADGDLVLPGLVLIAPGGLRHMEIERAGGVCRVRLVAGAPVSYSRPSVDRMFLSLAAAAGPRVSAALLTGMGRDGAAGLLAIRRAGGRTFAQDEGSSAVFGMPLAARDLRAAEEILTLDDIPAP",
    "response#2": "ILIGASTGGVSALEAVLPKLDPDGAPVVIVQHMPGNFLESFSQRLDRQLPQNVKLAEEGVPLRRGDVILAPGIGKHTEVVRRMDGWQCRFVANDPAALHCPSVDTLFLSAVSEAKKVSVAILTGLGKDGAQGMLKLAEAGATTFGQDEETSVVYGMPRVAFGLGAVQHQLPIHKIGEAC",
    "response#3": "ALGASTGGVQALSRILPFFPAQSPGLLVVQHMPEGFTAAFARRLDALCRMRVREAADGDLVLPGLVLIAPGGLRHMEIERAGGVCRVRLVAGAPVSYSRPSVDRMFLSLAAAAGPRVSAALLTGMGRDGAAGLLAIRRAGGRTFAQDEGSSAVFGMPLAARDLRAAEEILTLDDIPATR"
  },
  {
    "reference": "MNSKLAKVLHPVAGKPMIEYGVDAAKFAGVEKTVVVVGYQGDQLRALLKDRVTFAEQQIQRGTGDAVKCARSSLEKDAANILVLYSDMPLLKPETINALVENHLAADAALTLLTVVSDDPMNFGRIVRDAAGNAVRIVEEIEATSDELQIKELNPGVYCFNAQWLWHNLEQLKPSPKKGEYYLTDLLEMAVAQRVRVETMTIYDPLQCVGINTRVQLAYAERLMRERINERVMLNGATLIDPASTFIDAEVKIGQDTVILPNTHLQGKTTIGRDCKIGPGTIISNSVIGDACTIGPSLIESSTLEDSVEMGPFCHLRPNVYLSRHVHLGNYAELKNSRLGEGVHMGHFSYVGDAQVGKFTNIGAGTITCNYDGKNKHRTVIGDYAFIGSDSMLVAPLTIGNNARTGAGAVITKDVPPNSLAVGVPARVIRKNADEQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be optimized for activity in Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The desired protein should be able to perform acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate3. The designed protein must possess UDP-N-acetylglucosamine diphosphorylase activity to support regulation of cell shape, cell wall organization, cell morphogenesis under certain conditions.4. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLTADVEDPTGYGRILRDPSGAVRAIVEEKDASPEQRTIREVNTGVYVFRAGALRKHLPLVGTANAQGEKYLTDVIGLAQRDGMAIAPVVAERPEEVIGVNDRVQLAEATAVLRARTVRRWMLAGVTMISPDTIFIDADVTLSPDVTIYPGTHLRGKTKVAEDVVLGPNAIVSDCTIGRGSTIENSTVKESTIGDYTNVGPYAYMRPGSVVGDHVKVGDFVELKNVTIGNHTKISHLSYVGDGEVGDNVNIGCGVVFVNYDGQRKHHTKVEDNAFIGCNANLVAPVSVGRGAYVAAGTTVTREVPADALAVGRVRGPRVVVGARSRIGDFSHLEGGCVIGSDVILGPFAHLRDGAVIADGARIGNFVEVKDSTVGEGSKVPHLSYIGDTAIGRDVVIEPQVFFGPGVSIGDRVTIHGFSHIEGVMLRDPNRFDLRGTLQCGMDVEIDANVIIQGNVTIGDNVTIGAGCVLIDCEIDDNTLVRPYSIIESATVAEDCTVGPFTRLRPGAELRNDSHVGNFVEVKNARLGEGSKANHLTYLGDAEIGQRVNVGAGVITCNYDGANKFKTIIGDDVFVGSDSQLIAPVTIADGVTVGAGTTLTKDVEANQLVITRADVPAGAVVPARSNFTRKSGWEKRR",
    "response#2": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLTAVDPKDDSGYGRILRDADGNVSAIVEHKDASEAQKAIDEINTSVYAFKTKQLFAALKQVKCDNAQGEYYLTDIIKLAADEQLTIMTTTSLDDILGINDRKQLAEATAIMQQRINTYWMDQGVTLVDPRRIDVRGKLACGRDVLIDINAVFEGEVELGDGVQIGANAVIRDTSIGAGTVIHPHCVLEQSRIGANCQIGPYARTRPGVEMADDAKLGNFVEIKKSRIGKGSKINHLSYIGDAIIGANVNVGAGTITCNYDGANKHRTTIGDGAFIGSGVELVAPVEIGAGATVGAGSTITKAVPADELVLERSQQHTIEGWARPKK",
    "response#3": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLTVDMADPTGYGRILRDGDAVTAIVEQKDASAEQKQIKEVNTGILVAPTKHLKSWLARLSSNNAQGEYYLTDIVAMAVHDGVPVVTAQPSDEQEVLGVNSPLQLAALERCYQLRQAESLMLAGVTLVDPRRIDVRGKLACGRDVLIDINAVFEGEVELGDGVQIGANAVIRDTSIGAGTVIHPHCVLEQSRIGANCQIGPYARTRPGVEMADDAKLGNFVEIKKSRIGKGSKINHLSYIGDAIIGANVNVGAGTITCNYDGANKHRTTIGDGAFIGSGVELVAPVEIGAGATVGAGSTITKAVPADELVLERSQQRTIEGWKRPKKK"
  },
  {
    "reference": "MKHITIIGAGLAGTLCGLYLARRGYEVELFESRPDIRNSPTDYGRSINLALSCRGITALKAMNLLSEVNKIMVPMRARAIHEANGEIHYQPFGRHIDEYINAISRSDLNALLLNKAKLCPNIKLHFNMKLHSLDIHNKKIKFENKNGDFVEASYHRLIGADGAPSHVRDMLKNEGIVSASRDFLSHGYKELSISKKHTKGMAREHLHLWPRDSFMLLGNPNPDDSITGSLFLANEGKDSFAELNNEEKLHLFFKTQFPDAYAAMPNLVQEFFGNPTGHLSTIQCSPWYYKDECLLIGDAAHGIIPFFGQGMNSAFEDCRILDELLDEYQDDWSRVNSVFYEHRKVNTDAIAKMSMDNYHEIHSDIRNPKFILQKQIERELMLRYPEHYVSMHVLVMFTNTPYAKAMAIGELQSGLLEQICFPITDIKELNWQEVEKLLSLYDKKLAKII",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein requires FAD to activate a critical catalytic site.2. A protein that is capable of catalyzing reactions within the NAD(+) biosynthesis, Cofactor biosynthesis, quinolinate from L-kynurenine: step 1/3 pathway with high efficiency and specificity.3. The designed protein should be optimized for FAD binding activity in order to improve 'de novo' NAD biosynthetic process from tryptophan, anthranilate metabolic process, quinolinate biosynthetic process.4. The protein must exhibit the following characteristics: Required for synthesis of quinolinic acid.; Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn).5. The protein should be designed to have high stability and activity towards H(+) + L-kynurenine + NADPH + O2 = 3-hydroxy-L-kynurenine +   H2O + NADP(+). The designed protein sequence is ",
    "response#1": "MIEVTVVGAGLVGCLAALAFADRGHKVALYDARPDLRSEAELKNASLRSINLAVSARGIEALRSVDTKMAERVLADIIPMYGRMIHDLQGGQHAQAYGLWGECINSIDRAQLNRTMLDVIEDNANITFFPEHKLTNISLSRKDKKYQRPTSTFETKEGEERVVESDYIIGADGAFSKTRDRLQRYVRMNYAQQYIDCVYLELKIPKADGPDPFSISPNHLHIWPRHKYMLIALANGDGSFTSTLFAPPALMEQVCESQNTFISFFKEQFPDAYELMGESQILESYENNPRSPLVSLKCSPYNHKGECLLVGDAAHCMVPFYGQGMNAGFEDIRVLMEILDEKKWNVEEAFNTYTERRHKDLVAIVEATGRKAEEVKRALEVTVVGAGLVGCLAALAFADRGHKVALYDARPDLRSEAELKNASLRSINLAVSARGIEALRSVDTKMAERVLADIIPMYGRMIHDLQGGQHAQAYGLWGECINSIDRAQLNRTMLDVIEDNANITFFPEHKLTNISLSRKDKKYQRPTSTFETKEGEERVVESDYIIGADGAFSKTRDRLQRYVRMNYAQQYIDCVYLELKIPKADGPDPFSISPNHLHIWPRHKYMLIALANGDGSFTSTLFAPPALMEQVCESQNTFISFFKEQFPDAYELMGESQILESYENNPRSPLVSLKCSPYNHKGECLLVGDAAHCMVPFYGQGMNAGFEDIRVLMEILDEKKWNVEEAFNTYTERRHKDLVAIV",
    "response#2": "LIGAGLAGCLLAILLSRRGWQITLYERRGDPRIKGYESGRSINLALAERGRHALRQAGAEDAVMAKAVMMRGRMIHPVSGEPQLQRYGRDDSEVIWSIHRAALNVTLLDLAEQAGARVHFYRRLHTVDFDAGYARFIDDRDDQPHEIHFQALVGSDGAGSALRAAMQRKAASGRCVVVGLAGSGKSTLAAALQERAGVPAVSLDHVAWMPGLPQWSAPEAHARQHAVMLDVLGRGDGWVMEGSYLGAAMPLARRADCLVWLDLPVETCLARVLRRTVTQVVQRWVRWPDRPDLVEGFKQVFGLLWHYQRYHRDRCLPMLEAHPGLRTLHLRTCAEVDAALDRLEAP",
    "response#3": "MEVTVVGAGLVGCLAALAFADRGHKVALYDARPDLRSEAELKNASLRSINLAVSARGIEALRSVDTKMAERVLADIIPMYGRMIHDLQGGQHAQAYGLWGECINSIDRAQLNRTMLDVIEDNANITFFPEHKLTNISLSRKDKKYQRPTSTFETKEGEERVVESDYIIGADGAFSKTRDRLQRYVRMNYAQQYIDCVYLELKIPKADGPDPFSISPNHLHIWPRHKYMLIALANGDGSFTSTLFAPPALMEQVCESQNTFISFFKEQFPDAYELMGESQILESYENNPRSPLVSLKCSPYNHKGECLLVGDAAHCMVPFYGQGMNAGFEDIRVLMEILDEKKWNVEEAFNTYTERRHKDLVAIVRGNSIFWAEG"
  },
  {
    "reference": "MKVALGVSGGIACYKSAEILRRLQDRGVEVVVLMTKGATQFVTPLTFRALSGNKVYVDIFEGARSGADFEGAFDHILVAHSTDLFLVAPATADCIARLAIGLADDFLTTFHLAVTAPVVVAPAMNTRMWEHASVRANIETLKNRGVHIIEPEVGQMACRTYGPGRLAPVEEIVEVVMGILNRRRDLEGRKILVTAGPTVEDIDPVRYISNRSSGKMGYALAQAAQERGAQVTLVSGPIADATELHFPNTVHVRTTEEMRRAVVDHAADAEVVIKAAAPLDFRPKAVATQKIKKKTSDLTLEFEPTIDILKELGNSKNGKILVGFAAETENHFQNGLEKLKSKNLDLIVVNPASGPDSAFDSDMNHATIIDASGQSEEIPLVTKHVMADRILDRVVQLLK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein requires Mg(2+) to activate a critical catalytic site.2. The protein must have high specificity towards metal ion binding. The designed protein sequence is ",
    "response#1": "MLYCGSPPYPGSAFLAGNAAGRSGAGLVTLAVTQQMLPIYASAFHEGTFSLLPEEATGSFERASSIMKSLEGYRALLIGPGLGQSPYTREVILELLESLRALPDEERPQLVIDADGLNNLSALELWWTLLPKGTVITPHPGEMGRLCHGLKVSGGEIDRLELARRKAEQWQVTLVLKGTCTIIIEPGDRTHIL",
    "response#2": "MLYCGSPPYPGSAFLAGNAAGRSGAGLVTLAVTQQMLPIYASAFHEGTFSLLPEEATGSFERASSIMKSLEGYRALLIGPGLGQSPYTREVILELLESLRALPDEERPQLVIDADGLNNLSALELWWTLLPKGTVITPHPGEMGRLCHGLKVSGGEIDRLELARRKAEQWQVTLVLKGTCTIIIEPGDRTHIF",
    "response#3": "MVVAQSGGPSPVINNTLRGIVEAARNYANIGTVYGARHGIEGVLKEELLDLSSQHPDEISLLRYTPAAGSIGTCRYKLKPNQTEDFERVIEVLKAHNVGYFVYIGGNDSMDTANKVAKLAHERGLDLIGIGGPKTIDNDVGDSEFKLIDHTPGYGSCAKYWMHAVQNANEENQGSCPADPVLVMQAMGRKIGFIPAAARLADPHREMPLQIYLAESPCSLEQLADNVNDQLKRDGRCMVVISEGFNVGSLGERRDSFGHVQFSSSETTVAQTVVNYLNSKGLAAKGAARGNVPGTDQG"
  },
  {
    "reference": "MKTVLITGFEPFGGEQVNPSWEVVSRLDNAIIAGCRVVARQLPCVFGDSLTVLNAAIDALSPVLVLAIGQAGGRTDITVERVAINIDDARIPDNRGRQPVDEPIVAEGPAAWFSTLPIKAMVSAMREAGIPASVSQTAGTFVCNHVMYGLLHKLRDIQDVKGGFIHIPYLPEQAAAHPGAPSMAAETVRQALEIAIVTALHVETDIAVTGGATH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Removes 5-oxoproline from various penultimate amino acid residues except L-proline.2. The protein should have a low Km value for Release of an N-terminal pyroglutamyl group from a   polypeptide, the second amino acid generally not being Pro..3. The designed protein must possess pyroglutamyl-peptidase activity to support proteolysis under certain conditions. The designed protein sequence is ",
    "response#1": "SILQVTSQTNVNRMWQNDLHPILIERYPGSPGSYAVRQHIKHRLQGLQAGWLVEEDTFQSHTPYGYRTFSNIISTLNPLAKRHLVVACHYDSKYFLPQLDGKVFVGATDSAVPCAMMLELARSLDRQLSFLKQSSLPPKADLSLKLIFFDGEEAFVRWSPSDSLYGSRSLAQKMASTPHPPGARNTNQIQGIDLFVLLDLIGARNPVFPVYFLNTARWFGRLEAIEQSLHDLGLLNNYSSERQYFRSNIRRYPVEDDHIPFLRRGVPILHLIPSPFPRVWHTMEDNEENLDKPTIDNISKILQVFVLEG",
    "response#2": "SILQVTSQTNVNRMWQNDLHPILIERYPGSPGSYAVRQHIKHRLQGLQAGWLVEEDTFQSHTPYGYRTFSNIISTLNPLAKRHLVVACHYDSKYFLPQLDGKVFVGATDSAVPCAMMLELARSLDRQLSFLKQSSLPPKADLSLKLIFFDGEEAFVRWSPSDSLYGSRSLAQKMASTPHPPGARNTNQIQGIDLFVLLDLIGARNPVFPVYFLNTARWFGRLEAIEQSLHDLGLLNNYSSERQYFRSNIRRYPVEDDHIPFLRRGVPILHLIPSPFPRVWHTMEDNEENLDKPTIDNISKILQVFVLEQG",
    "response#3": "MKFNQFAHVKVPFEQKLAELNRIAFLHAGDEDLASNHIYRLFLERAFPNFKTEAAKNHALSNLAATENADILTYLNSSKINARVFYAVGLQLLGFEAELDFDLKDPFSAMDKLNLPYQKEIHHRDDVINAWYDLLCTSTKKGQNLLDILANRGY"
  },
  {
    "reference": "MTVNLLNYSQPQLAEYFHGLGEKPFRAKQLMRWMHHFGVHDFDQMTDIAKSLRDKLKDQAEITPPGIKLEQISEDGTRKWLIDAGTGNGVETVFIPEAERGTLCVSSQVGCALECTFCSTGRQGFNRNLTVSEIIGQLWVANKALGRDPKGDRIISNVVMMGMGEPLANFDNVVTALNIMLDDSAYGLSRRRVTVSTSGMVPAMDRLREACPVALAVSLHAPNDALRDVIVPINKKYPIKELMAACQRYLEKAPRDFVTFEYVMLDGVNDSVEHARQLLDIVRDVPCKFNLIPFNPFPNSGYDTSKPEAIRRFRDVLMQADYVVTTRKTRGDDIDAACGQLAGKVQDKTRRTERNKVMQVISVEKTV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have a binding site that is specific to the target S-adenosyl-L-methionine ligand.2. The protein should have 4 iron, 4 sulfur cluster binding, metal ion binding and be active in cytoplasm.3. The protein requires [4Fe-4S] cluster to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "STMVGCPAKCAFCATGSMGFGRNLTPGEVVGQILAVSWHQGFSPREMRNLVFMGMGEPLLNYDHTIAAAKLLLHPQALGMSKRRVTLSTVGLAKGIRKLAAEDELGIKLAISLHAPDEETRQQIIPTGQANSISEIMAAARDYQAVTGRRVTFEYAMLSGVNDS",
    "response#2": "AYEQIRASVTTHRGCFGGCSFCAITHHQGKVIQSRSERSVLAEVERMAAMAWFRGSVSDVGGPTANMYGVHCGNTRGGHACRRESCLYPSPCRYLAVGGERGAALLRAVRGVRGVRNVAVSSGIRYDLMERQPAYFRELVAHHVGGLLKVAPEHMVARVTDLMRKPGKESFDRFLERFRRESARLGKKQYIIPYLMSGHPGCTLDDMVELALFLKRAGLRVEQVQDFTPTPGTLSTCMYHTGLDPFAGAPVHVPRGDREKRLQKALLLWHPR",
    "response#3": "MCPNNALKQCVKEYTVDELMTILKRDFNNWGSDGGVTFTGGDPLMHHEFLVEVLKKCYDSQIHKAIETSGYAKQEVFLEVLKYIDFAFIDVKNMDREKHKQGTGVYNDLILSNIEALKKSNWNGRLVLRQPTIAGYNDSDENAYKLI"
  },
  {
    "reference": "MAEITEVRIYKSKGNGSVKAYASVSFDNEFVVKGLKVVEGEKGLWVSMPSRRMKDGSFQDVFHPVSREARDKIVDAVLKAYQEQE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be able to modulate division septum assembly in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "PMMPKSWFFDTQSVSCDPENSDIVTLQTPLGMAKFIVIENSGCASLCMMVDVEPLALSSTKEIRFCDTIKVMNNRITAYEC",
    "response#2": "PMMPKSWFFDTQSVSCDPENSDIVTLQTPLGMAKFIVIENSGCASLCMMVDVEPLALSSTKEIRFCDTIKVMNNRITAYEC",
    "response#3": "PMMPKSWFFDTQSVSCDPENSDIVTLQTPLGMAKFIVIENSGCASLCMMVDVEPLALSSTKEIRFCDTIKVMNNRITAYEC"
  },
  {
    "reference": "MVKAEHESRALAIFGTASDVGKSIVATALCRIFNNAGIDVAPYKAQNMSNNSGVTPDGCEIGRAQIVQAEAARVVPTADMNPVLLKPNSDTGAQVVLQGKVCSTETAKGYFRDTSLWAEAARESLERLMKRHEVVVIEGAGSCAEMNLYDRDFVNFRTARLSGASVILVADIDRGGVFGQVVGTLAVLPAEDRALVKGVIINRFRGDIDLFRDGVEMLESMTGIPVLGVIPYFRGFAIDAEDAVPLSAKVDPAGGPEEGKIGVAAIYFPHISNFTDLSPLEHDPAVELHYLHYPRSLKGYKALILPGSKNVRGDLAWLYSLGWEAEIRAFRAEGGIIMGICGGYQMLGNSIADPYGVEGSAGTTKALALLDVETVLEQDKCLANARGTVIGTSAEASGYEIHMGRSVVSDLCTPFIRVTARNNRPEDELDGAVSSDGRVMGSYFHALFDESSVKQWFLSLLEPSYRLQKHEKGRQESYELLADHFSSHLDLNKIFTIIDKVHS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have ABC-type vitamin B12 transporter activity, catalytic activity to facilitate glutamine metabolic process.2. A protein that can modulate the overall flux of metabolites through the Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed.3. The Nucleophile, active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.4. The protein must exhibit the following characteristics: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide; NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation..5. The presence of GATase cobBQ-type domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MIQGTTSDAGKSTLVAGLCRLARRAGVRVAPFKPQNMALNSAVTIDGGEIGRAQALQAVAAGIDAHTDLNPVLLKPTSDRGAQVIIHGKARMNLDARAYHDYKPVAFEAVLASYARLKASYDTIFVEGAGSPAEINLRERDIANMGFAEAVDCPVVLVSDIDRGGVFAHLTGTLACLSDSEQARVRGFVINRFRGDVSLLQPGLDWLEAKTGKPVLGVVMVLGTSSGAGKSLMTAALCRVLKRRGETPLPFKGQNMSNNAWVDPGGGEMAYSQALQAWAAGREPECAMNPVLLKPQGDSTSEVIHLGRSVGTCRAEHYYRDWFRPGWAAIRQGLHTLENENPEGRLVLEGAGSPVEVNLQARDLTNLRLAQYLRARCLLVADIERGGVFAQIVGTLALLRPVERPLISGLLINRFRGRRELFDEGRRWLEQHTGIPVLGVMPWLDEPDDIEAILVAAIYFPHISNFTDLSPLELDPKVELHYLHFPRSLRGYQALILPGTKNVRGDLDWLTSLGWAEKIREFRRDGGLIMGICGGYQMLGATIADPSGVEGEPGESAGLGMLPVHTVLEEEKCLSNAIGNIQGESIAVSGYEIHMGRTTSNGDCSSFLRVTARNNRPADDVDGVITPDGKVIGTYFHGIIDEPEVRCWFREP",
    "response#2": "MIQGTTSDAGKSTLVAGLCRLARRAGVRVAPFKPQNMALNSAVTIDGGEIGRAQALQAVAAGIDAHTDLNPVLLKPTSDRGAQVIIHGKARMNLDARAYHDYKPVAFEAVLASYARLKASYDTIFVEGAGSPAEINLRERDIANMGFAEAVDCPVVLVSDIDRGGVFAHLTGTLACLSDSEQARVRGFVINRFRGDVSLLQPGLDWLEAKTGKPVLGVVAALTGDVDDALRVVVPALPHISNHTDFDALRAHPQVDFHYVRAGSAPPPADLIILPGSKNVQGDLAFLRAQGWDAVLQRHLRYGGRVIGICGGMQMLGREVADPYGVEGPPGTVEGLGWLDFSTTLTRDKTLKNVTGRLATDAGRIAGYEIHMGETQGPALDAPALLLADEMGGVRPDGARSADGQILATYVHGLFDTPAGL",
    "response#3": "MIQGTTSDAGKSTLVAGLCRLARRAGVRVAPFKPQNMALNSAVTIDGGEIGRAQALQAVAAGIDAHTDLNPVLLKPTSDRGAQVIIHGKARMNLDARAYHDYKPVAFEAVLASYARLKASYDTIFVEGAGSPAEINLRERDIANMGFAEAVDCPVVLVSDIDRGGVFAHLTGTLACLSDSEQARVRGFVINRFRGDVSLLQPGLDWLEAKTGKPVLGVVPYLHGLTPVVVPFLEATPDEQLQIIRAENARRGLQVPPAVLEYLARRISTNIRELEGALIRVTAFASLNRQPITVPLAEQVLRDLIPSGDAPERIACLTLSRIANFDDLDPLAAEPGVRMVMVQPGQPIPAEARLVILPGSKSTRGDLAFLREQGWDIDLAAHVRRGGHVLGICGGYQMLGRSVADPEGIEGPAGTTPGLGLLDVETVMTPDKRLTRVRATHAGSGLAVEGYEIHIGRTEGADRARPFAIVEGQNEGAMSADGRVIGSYLHGLFGADRVRALIAQRLQLDTLMRDLEDRL"
  },
  {
    "reference": "MGDKRQVPAKSKIIAATRNRGKLEEIAQLLAGLPYEVISMTEAGVTGDIAENGDTFEENALIKAREVRRMTGEAVMADDSGLEVDHLGGAPGIHSARYAGEEATDEDRIRKLLTELSGVPADRRTARFICAIALILPDGREIVVKGTCEGFITTEPAGKGGFGYDPVFYVPEFGMTFAQMDEELKNSISHRGNALRKILDILSEEAEKH",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must have high specificity towards ITP diphosphatase activity. The designed protein sequence is ",
    "response#1": "GELRPIKITRNWLDHAAGSVLVEXGKTRVLCAASASXGVPRWRTGSGLGWVTAEYAMLPSSTHTRSDRESVKGRIGGRTHEISRLIGRSLRAVIDYKALXENTIVLDCDVLQADGGXRTAAITGAYVALADLL",
    "response#2": "ELRPIKITRNWLDHAAGSVLVEXGKTRVLCAASASXGVPRWRTGSGLGWVTAEYAMLPSSTHTRSDRESVKGRIGGRTHEISRLIGRSLRAVIDYKALXENTIVLDCDVLQADGGXRTAAITGAYVALADIL",
    "response#3": "MSLNL"
  },
  {
    "reference": "MSPSAPANSAPDPDRNGVPDVGPASAAPPDLQTAAARAAWLRSTLQHHAHLYYVLDAPELPDAEYDRLFQELQAIEAMYPALRTADSPTQRVLGQVRDGFVVVRHAVPMLSIRTETDTEASGAVAFDARVRRELELPDDAPPISYATELKFDGLAINLRYEHGVLVQAATRGDGESGEDVTQNIRTIGQIPLRLRWAPTLGADAHSLPPEGARFALGRPGGETGAPDLAPAVLEVRGEVYMRRDDFEALNERQRERIARGDKGEKTFVNPRNAAAGAVRQLDPAIAAQRPLSFFAYGLGEVQGWALPHTHAGLLDALAAAGLPVCAERAVVQGADGLVAFHQRIGALRDSLAFDIDGVVYKVDSLALQQRLGFVTREPRWAVAHKYPAQEQMTRLLGIEIQVGRTGKLTPVARLEPVFVGGTTVSNATLHNEDEARRKDVRVGDTVIVRRAGDVIPQVVGVVLEQRPDDVGEPFDLYQRLGGRCPVCGSAIARPEGEADWRCTGGLFCAAQRKQAILHFASRRMMDIEGLGDKLVDQLVDAGVIRTLPELYKLGVAKLTALERMGEKSAVNLVAALEKSKQTTLARFLFSLGIRQVGEATAKALARHFGALDRVMDASVDQLQAVPDVGPIVALSIRTFFDQPHNREVVEQLRAAGIHWAEHEASAAADAAELPLAGKTLVLTGTLPTLGRDAAKDLIEAAGGKVSGSVSKKTHFVVAGAEAGSKLDKARELGLVILDEAGLLALLNEAEADADADAEGLPDSPVAPT",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design must be able to perform DNA binding, DNA ligase (NAD+) activity, metal ion binding in order to contribute to DNA replication.2. The protein have enhanced NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-   (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-   nicotinamide D-nucleotide..3. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding.4. The designed protein have BRCT domains with specific sequence motifs that are crucial for its activity.5. For general function, the protein need meet that It is essential for DNA replication and repair of damaged DNA. The designed protein sequence is ",
    "response#1": "MSKKRHEELKKIISEHDHNYHVLDKPTITDYEYDQLFAELLDIEKNPKGLDLSDSPSQRVGGTVLEGFTKAQHRLPMLSLANSYSPEDIFEFDERVRKFLNTEDPVEYLCELKFDGLSMELIYENGQLVRAITRGDGTVGEDVTHNIKTIKSIPLKLSHKNPPPLLEVRGEVLMFKEDFARLNETQQENGQQTFANPRNAAAGTVRQLDSRIAASRPLRFFGYALGAVEGETFNTQKNIQEYFNDHGIPTVLPYKEDLLVVAKGPEEVVKYYHHIEKVRPKLPFDIDGVVIKVNSLRLQEDLGLVARSPRWATAAKFKPEQAQTTVEDIVVQVGRTGALTPVAIMKPVKVGGVTVTNATLHNQDEITRKDIRIGDTVIIQRAGDVIPEVVEVVNPDKRPADRLPYSIPERCPACDSVAVKAEGEVVTRCVNPLCIAVVKESLKHFVARRTLLESVDFQVGRTGAVTPVARLKPIFVGGVTVSNATLHNADEIARLGVKIGDTVIIRRAGDVIPQIVAIVPEKRPDASRITDILTSVGRTGKITYIANILPVLLNQTTVKAATLHNHNFIKDMNININDEVNIIKAGEIIPKVISLKEEKESRITDILTSVGRTGKITYIANILPVLLNQTTVKAATLHNHNFIKDMNININDEVNIIKAGEIIPKVISLKEEKR",
    "response#2": "MSKKRHEELKKIISEHDHNYHVLDKPTITDYEYDQLFAELLDIEKNPKGLDLSDSPSQRVGGTVLEGFTKAQHRLPMLSLANSYSPEDIFEFDERVRKFLNTEDPVEYLCELKFDGLSMELIYENGQLVRAITRGDGTVGEDVTHNIKTIKSIPLKLSHKNPPPLLEVRGEVLMFKEDFARLNETQQENGQQTFANPRNAAAGTVRQLDSRIAASRPLRFFGYALGAVEGETFNTQKNIQEYFNDHGIPTVLPYKEDLLVVAKGPEEVVKYYHHIEKVRPKLPFDIDGVVIKVNSLRLQEDLGLVARSPRWATAAKFKPEQAQTTVEDIVVQVGRTGALTPVAIMKPVKVGGVTVTNATLHNQDEITRKDIRIGDTVIIQRAGDVIPEVVEVVNPDKRPADRLPYSIPERCPACDSVAVKAEGEVVTRCVNPLCIAVVKESLKHFVARRSGFTPVVHDKPMLSLNNGFADEDVIAFDKRVADGLDKATDLAGTVTEPVEYACELKFDGLAISLRYENGRFVQASTRGDGTTGEDVTENIRTIRAIPLTLKGKRIPRMLDVRGEVLMFKRDFARLNERQRAAGQREFANPRNAAAGSLRQLDSKITASRPLSFFAYGIGVLDGADMPDTHSGLLDWYETLGLPVNRERAVVRGAAGLLAFFHSVGERRESLPYDIDGVVYKVNRRDEQDRLGFVSRAPRFALAHKFPAEPLALDRFIFALGIRQIGEATAKRLARHYGSFGAWRAAMLAGTEEARAELTSIEDIGPSVAGDLLDFFSEEHNVQRA",
    "response#3": "MSKKRHEELKKIISEHDHNYHVLDKPTITDYEYDQLFAELLDIEKNPKGLDLSDSPSQRVGGTVLEGFTKAQHRLPMLSLANSYSPEDIFEFDERVRKFLNTEDPVEYLCELKFDGLSMELIYENGQLVRAITRGDGTVGEDVTHNIKTIKSIPLKLSHKNPPPLLEVRGEVLMFKEDFARLNETQQENGQQTFANPRNAAAGTVRQLDSRIAASRPLRFFGYALGAVEGETFNTQKNIQEYFNDHGIPTVLPYKEDLLVVAKGPEEVVKYYHHIEKVRPKLPFDIDGVVIKVNSLRLQEDLGLVARSPRWATAAKFKPEQAQTTVEDIVVQVGRTGALTPVAIMKPVKVGGVTVTNATLHNQDEITRKDIRIGDTVIIQRAGDVIPEVVEVVNPDKRPADRLPYSIPERCPACDSVAVKAEGEVVTRCVNPLCIAVVKESLKHFVARRAMDIDGLGEKIIDQLVEKGLIRSPADLYRLEIERLLEFDRIGEVSATRMIEAIERSKAQTLARFLYALGISEVGEATAANLAAHFGTLEALMAADIDTLITVPDVGKVVASHVRNFLDEESNREIIQRLENFEGWGKRSTEKLFEALPGELQVPQGVLAGKTVVLTGTLPTLTREAAKEMLEAAGAKVAGSVSKKTDYVVAGADAGSKLAKAEELGIPVLDEAGMHTLLEGE"
  },
  {
    "reference": "QNAHPSPKVPP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that localizes to extracellular region and can perform metalloendopeptidase inhibitor activity with high specificity and efficiency.2. The protein must exhibit the following characteristics: It inhibits angiotensin converting enzyme (ACE) activity with a K(i)app of 82.10 uM.; It has a bradykinin-potentiating effect (75%), when 60 nmol of BPP-11f are coinjected with 0.5 ug of bradykinin into rats. The designed protein sequence is ",
    "response#1": "LLKASHVRDAVAVIRYLAWLEKNVPTGTVDEFSGAKRVEEFRGEEEFFSGPSFETISASGLNAALAHYSPTKELHRKLSSDEMYLLDSGGQYWDGTTDITRTVHWGTPSAFQKEAYTRVLIGNIDLSRLVFPAATSGRVVEAFARKALWDVGLNYGHGTGHGIGNFLCVHEWPVGFQYGNIPMAEGMFTSIEPGYYQDGEFGIRLEDVALVH",
    "response#2": "LTALREQMLTQNLSAYIIPDTDAHMSEYIGECDQRRAWITGFIGSAGIAVVTERKAALWTDSRYWTQAERQMDCNWELHKEVSTGHIVTWLLTEIPVGGRVGFDPFLFSIDSWESYDVALQDADRELVSIL",
    "response#3": "GMRRAHIKDAVALCELFNWLEKEVPKGGVTEISAADKAEEFRRQQADFVDLSFPTISSTGPNGAIIHYAPVPETNRTLSLDEVYLIDSGAQYKDGTTDVTRTMHFGTPTAYEKECFTYVLKGHIAVSAAVFPTGTKGHLLDSFARSALWDSGLDYLHGTGHGVGSFLNVHEGPCGISYKTFSDEPLEAGMIVTDEPGYYEDGAFGIRIENVVLVVPVKTKYNC"
  },
  {
    "reference": "MANSRRVSRVSSLIKREVSQMLLHDIKDDRVGAGMVSVTEVDVSGDLQHATIFVSIYGSEQAQAETMEGLKSSTSFVRRELSHRMRLRRSPEVVFRQDHSLERGDRMVHLLNQIKDTIPEEEGITEEE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: May interact with the 5'-terminal helix region of 16S rRNA.; Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes).2. The protein should have a specific function in maturation of SSU-rRNA and should be localized to cytoplasm in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "MITTN",
    "response#2": "STGFKAGVKDYKLT",
    "response#3": "MLQVY"
  },
  {
    "reference": "MNDSSTSNRKALFEEDKDLKVNKRADALLERKQQEAEFELEKLQAEERGEDFDRKRAWDWTVKETEEWKEKKERKRERESQSGVHDMSSTAQRAYEKDLASFKPDLETYEKEKETGLHHTPSFNHKPTPEALDRLVNGLTKGDKQRMKRRKQAGADDQHATYISDKNKQFNEKLNRQYDKYTKEIRDNFERGTAL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be expressed specifically in the post-mRNA release spliceosomal complex2. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.3. The protein must exhibit the following characteristics: Involved in pre-mRNA splicing.. The designed protein sequence is ",
    "response#1": "EYGTSDLVREVYGLAVETLGEDFMDEKLFIAYARFETKLKEYERARAIYKYALDRLPRSKSITLHKAYTTFEKQFGDREGVENVILAKRRVQYL",
    "response#2": "EYGTSDLVREVYGLAVETLGEDFMDEKLFIAYARFETKLKEYERARAIYKYALDRLPRSKSITLHKAYTTFEKQFGDREGVENVILAKRRVQYF",
    "response#3": "EYGTSDLVREVYGLAVETLGEDFMDEKLFIAYARFETKLKEYERARAIYKYALDRLPRSKSITLHKAYTTFEKQFGDREGVENVILAKRRVQYA"
  },
  {
    "reference": "MQRFSLVTHRSFSHSCVKPKSACSLVKPVHHLVKIDKSKLSPRFPELKYDKSDIRSPGFKPKDTHADRLNDHYLNTLQSDLLLINYSHNAAVVKGLKQRAWSGDSPYHLNRPPKNPRGSKAQLPDIHPIKWSNIPGLESVVINCFVREARENQLLAITAALQLQQITGCKPHPIFSKNDVPTWKLRKGHQMGAKVELKGKEMSQFLSTLTEIVLPRIREYKGISNQSGNRFGGISFGLTAEDIKFFPEIDANQDSWPKTFGMHININTSAQLDYQARTLLSGFQFPFFGEEK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein localizes to the mitochondrion, cytosolic large ribosomal subunit, mitochondrial inner membrane. The designed protein sequence is ",
    "response#1": "STLSRCLTCPSAKLPDKINNPTKGAAFVNTVPTNKYLDNHGLNIMGKNLLSYHVTKSIIQKYPRLPTVVLNAAVNAYISEAVLAHIAKYWGIEVETTSVLSRYLKMEPFEFTLGRLKFFNNSLNSKDGIELITGKNFSETSALAMSVRSIIAAIWAVTEQKDSQAVYRFIDDHIMSRKLDITKC",
    "response#2": "STLSRCLTCPSAKLPDKINNPTKGAAFVNTVPTNKYLDNHGLNIMGKNLLSYHVTKSIIQKYPRLPTVVLNAAVNAYISEAVLAHIAKYWGIEVETTSVLSRYLKMEPFEFTLGRLKFFNNSLNSKDGIELITGKNFSETSALAMSVRSIIAAIWAVTEQKDSQAVYRFIDDHIMSRKLDITKC",
    "response#3": "STLSRCLTCPSAKLPDKINNPTKGAAFVNTVPTNKYLDNHGLNIMGKNLLSYHVTKSIIQKYPRLPTVVLNAAVNAYISEAVLAHIAKYWGIEVETTSVLSRYLKMEPFEFTLGRLKFFNNSLNSKDGIELITGKNFSETSALAMSVRSIIAAIWAVTEQKDSQAVYRFIDDHIMSRKLDITKC"
  },
  {
    "reference": "MMTASQRYDTKIHRRVTRTVMVGDVPIGSEHPIAVQSMINEDTLDIDGSVAGILRLADAGCEIVRVTTPSIGHAKAMGKIRSALRAQGCKIPLVADVHHNGTRIALEVAKHVDKVRINPGLFVFDKPDPDRQEFSKEEFDAIGDRIKETFAPLVQVLKEQNKALRIGVNHGSLAERMLFTYGDTPQGMVESAMEFVRICDSLDFHNIVISMKASRAPVMLAAYRLMADTMDREGFNYPLHLGVTEAGDGDYGRIKSTAGIATLLAEGLGDTLRVSLTEAPEKEIPVCFSILQALGIRKTMVEYVACPSCGRTLFNLEEVLHQVRNATCHLTGLDIAVMGCIVNGPGEMADADYGYVGKGPGVIALYRNRDEIRKVPESEGVEALVQLIKDDGRWVEPD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for [4Fe-4S] cluster binding.2. The designed protein should have iron ion binding, 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity to facilitate isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway.3. For general function, the protein need meet that Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME- 2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.4. The protein design should consider the endogenous proteins and enzymes present in the isopentenyl diphosphate biosynthesis via DXP pathway, Isoprenoid biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MTLEGKVALVTGASRGIGRATAILLAKEGAKVVVNYSRSKAEAEEVVGMILKAGGQAVAIQADVSKEEDVQRMFEEAEKTWGGIDILVNNAGVARDNLVLRMKDEEWDAVMDVNAKGVFLCSQAAARIMRKQGEGGKIINIA",
    "response#2": "MPTISVIIPTYNAGRHLDRCLDSVVAQTWPNLEILVVDGGSTDGTLEILRRYAARGARLRVISAPDRGISDAFNRGIREARGEIVGILNSDDWYEPDALERVARAFADPEVAAVFGDCVTVDDRGRVLGAYRFPEVTLDRLRYWNALPHPATLFRRSVYERIGGFRDDLHFCMDYDYWLRAARHGRVARRIPEVLVYYRLHPDSASHTEAAVLQEARLVAERHLGEGLPVSAAAEARRLAELLERRQGPWRRQLLRWAFRRLFGPR",
    "response#3": "MNIILATNNLNKLREVKGIIGDEFNLLSLKDIGCDTDIPETADTIEGNALLKARAIKENTGMISLADDSGLCVDVLNGAPGIYSARYAGENATDRQNLEKLLTELSDIEDEQRTAHFYCAIVYMRHENDPEPIIAEGRWHGHILHEKRGDMGFGYDPILYLPEFGKTMAEMEPAEKNRISHRGKAMKKMKKYL"
  },
  {
    "reference": "MQRIQLNITGMSCSCCAPNGWNNLPNKLSDFSTLVNSATRVAAINLSEVTQTAEVCEAVRRAALCTDGGEALQRRQADADNARYLLIRLAVAAALFVPLAHLSVMFAVLPSTHFPGWEWMLTALAIPVVTWAAWPFHRVAIHNARYHGASMETLISTGITAATIWSLYTVFGHHQSTEHRGVWRALLGSDAIYFEVAAGITVFVLAGKYYTARAKSHASIALLALAALSAKDAAVLQPDGSEMVIPANELNEQQRFVVRPGQTIAADGLVIDGSATVSMSPITGEAKPVRVNPGAQVIGGTVVLNGRLIVEAAAVGDETQLAGMVRLVEQAQQQNANAQRLADRIASVFVPCVFAVAALTAVGWLIAGSGPDRVFSAAIAVLVIACPCALGLATPTAMMVASGRGAQLGILLKGHESFEATRAVDTVVFDKTGTLTTGQLKVSAVTAAPGWQANEVLQMAATVESASEHAVALAIAASTTHREPVANFRAVPGHGVSGTVAERAVRVGKPSWIASRCNSTTLVTARRNAELRGETAVFVEIDGEQCGVIAVADAVKASAADAVAALHDRGFRTALLTGDNPASAAAVASRIGIDEVIADILPEDKVDVIEQLRDRGHVVAMVGDGINDGPALARADLGMAIGRGTDVAIGAADIILVRDNLDVVPITLDLAAATMRTIKFNMVWAFGYNIAAIPIAAAGLLNPLVAGAAMAFSSFFVVSNSLRLRNFGAILSCGTSRHRTVKRWRCPPPTRLRSTACSPVDASPLRPV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. The designed protein have HMA domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "M",
    "response#2": "EIGS",
    "response#3": "MGRLP"
  },
  {
    "reference": "MATKFIFFTGGVVSSLGKGIACSSLGLLLKSRGFSVTILKLDPYINVDPGTMSPYQHGEVYVTEDGAETDLDLGHYERFIDSDMSRLNNTTTGQIYYNVIQKERRGDYLGATVQVIPHVTDAIKSHITKVADETQSDIVIVEIGGTVGDIESLPFLEALRQFRLQNAPEDTLVVHLTLVPFVAAADELKTKPTQHSVMRLREIGIQADVLLCRTDRILDEGLKEKIGLFCNVSPDAVKTALDVKTIYEVPLQYARSGFDELVIKRLNLPDRPRRIDALEQKITRYKNVKREIEIAICGKYVSLQDAYKSIIESFVHAGMENDVHVKLRWINTEELEETGLTDQLRGCGGILVPGGFGERGIEGKLMAIRHARENKIPFLGICLGLQCASIEFARNVLSLEQANSTEFNPDTPDPVIHLMESQKDISEKGGTMRLGSYPCILTEDTLAQKAYGQKEIRERHRHRWEFNNAYRERFEANGMRISGVSPDGTLVEMIELANHPWFVGCQFHPELKSRLSKAHPLFRDFVAAALQYETVRNHNIHAR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be optimized for glutaminase activity.2. The designed protein should contain one or more Glutamine amidotransferase domains that are essential for its function.3. The Mg(2+), UTP binding site should be stable and able to withstand changes in environmental conditions.4. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen5. A protein should be able to manipulate the activity of enzymes in the Pyrimidine metabolism pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "LPDAYKSIVEAFIHAGAVNECRVDIEWISSETITEENCHSILGKLDGVLVAPGFGERGVEGKIEAVRYIREKKIPFFGICLGMQCAVIEFARNVLKLKGASSTELNENTKHPVIDLMEEQKKVTDKGGTMRLGAYPCDLKKGSKASLVYGKAKISERHRHRYEFNNKYLKQMEDAGLMATGINPDTGLVEVVELKDHPWFIGTQYHPELKSTVLNPHPLFVKFVR",
    "response#2": "LPDAYKSINEAFVHAGAVNECKVDIEYIHSENLEEGNPEEQLKGLKGILIAPGFGDRGIEGKILAAKYARENNIPFLGICLGLQCAVIEFARNVLKMEKAHSTEMFHKTPHPVIDLMEEQKGITEKGGTMRLGAYACHIKKNTNAHRAYKTTEIQERHRHRYEFNSKYIDDFKNAGMSAVGINPDNSLVEIMEIPKHKWFVGVQFHPEYRSTVINPHPLFVDFIDAYKSIYESLDHAGFSHNSRVLVKRIEAEELEEQDPEIVLSGIDGLLVPGGFGYRGIEGKVKAIQFARECEIPYFGICLGMQCAVIEFARNRLGLAEANSSEFSAETPDPVICLLEEQKTVTDKGGTMRLGTQPCQLVENSRAAACYGTSEVSERHRHRYEFNPEYEQAFEKAGMIAAGKSPSGKLVEVVEIPAHPWFVAVQFHPEFKSKPHAAHPLFRGFVES",
    "response#3": "VKVAIVGKYTQLEDAYKSIKEALTHGGMANRVKVNVEWVDAETFDSEDAAPHLEGYHAILVPGGFGERGTEGKIKAAEFARTRNVPYLGICLGMQMAVIEAARNVAGMAKAGSEEFDHEAGQKRFEPVVYHLKEWVQGNHKVKRKADDDKGGTMRLGAYSATLIEGSKVAKVYGDTSIEERHRHRYEVDTKYREALEAEGLCFSGMSPDGRLPEIVEWTDHPWFIGVQFHPELKSKPFEPHPLFADFVAAALAI"
  },
  {
    "reference": "MQLMEYTQLALFLGLLALMSPVLGRFIGRAVLRGEQTWLHSVLGPVERLIYRASGIRPEARQSWRQYAASLCAFSAAGFLMTFGVLMLQDKLPLNPQGFPGLSWHLAFNTAVSFLTNTNWQSYAGESTVSHFSQLVGLAYHNFVSAAAGLAVAVAVMRGLASQESKTIGNFWADLVRSVLYVLLPISLVLAVVLVGQGVVQTMSAGTEVATLEGGHQFIAMGPTASQVAIKMLGTNGGGFFNANAAHPLENPTALSNLLQMLAIFIIPSSLVFTLGEAVSNRRHAWTVWFVMACLFMVGTLSLYKAETLGTPLLTEVAQAPVTNMEGKEVRFGIFGSAMFATVTTDASCGAVNAMHDSLTPLGGLVTLVNMQMGEVIFGGVGSGLYGMVLFILLTVFLAGLMVGRTPDYLGKRIEGREVTLAMLALIIAATPPLLFSAVAAVSGWGQAALNNAGAHGFSEILYAYTSGVQNNGSAFAGLNANSPAWNVTLALAMLIGRFGVMLPMLGVAGSMASRKARPIGEFSFPVSGFTFGLLLTLVIVIVGALTYLPALALGPVVEHFQMLDGLLH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: This subunit binds the periplasmic potassium ions and delivers the ions to the membrane domain of KdpB through an intramembrane tunnel.; Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm.2. The protein should be optimized for plasma membrane-specific folding and stability, while maintaining potassium ion binding. The designed protein sequence is ",
    "response#1": "SCGGH",
    "response#2": "SACSML",
    "response#3": "MPA"
  },
  {
    "reference": "MERGPLRVFSGSANQALAKSVAEELGQPLGNLDLGRFPDGEVSVQIGESVRGTDCFIIQPTCPPVNENLMELLILIDAFRRGSAERITVVMPYFGYARQDRKARGREPISAKLVANLIAEAGADRVLAVDLHTDQLQGFFDLPVDHLPARQVFADHFRHLGLRGEDAVIVSPDVGGVHQAKLLADELDAALGIVVKRRSGPGKNEVLEVIGEFSGRRVIIYDDMIQTGGTVIDATEALLDRGAREVHCAVTHPVFSGDASQRLAASPLTSLTVCDTIPVPEHKRFDRLAVLTVASLLADAIKRIHHDQSVSACLHTSHYHQPRLFGG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that contributes to ribonucleoside monophosphate biosynthetic process, nucleotide biosynthetic process by functioning in cytoplasm with magnesium ion binding.2. The protein should be designed to have high stability and activity towards ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-   diphosphate + AMP + H(+). The designed protein sequence is ",
    "response#1": "EDLVVVSPDVGGVARARAFAKKLSDAPLAIVDKRRHGHNVAEVMNLIGDVKGKVAVMVDDIIDTAGTIVKGAALLHEEGAREVYACCTHAVFSPPAIERLSSGLLQEVIVTNR",
    "response#2": "PVLLEYFQRTLTDADREKLTVVSPDTGRVRVADTWSDSLGAPLAIIHKRRDPNVANQVTVNEIVGEVEGRVCLLVDDMIDTGGTIVKAAEALKKNGAQKVIVAATHAVFSAPATERLQSEAIDDVVVTDPK",
    "response#3": "ARHFAQLELEDLTVVSADEGFAKKARRLADRLNAPLAIIEKRRLGNNGRIEAMGIIGNIEGRHALVVDDEIDTAGSLTQAVRVVHEHGARDIYCCATHGVFSGPAIERLSRAPIKEIVITDPL"
  },
  {
    "reference": "MNTTIAQQIADEGGVEAYLHAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVGYSEETFNTIREEYLTFAEQLPGNLDIRFVPLSALEGDNVASQSANIPWYSGPTLLEVLETVAIRRTVETQPMRFPVQYVNRPNLDFRGFSGTVASGSVKVGQRIKVLPSGVESSIARIVTFDGDLQEAAAGEAVTLVLKDEIDISRGDLLLDAQDTLPSVQRASVDVVWMAEQPLSAGQSYDIKVAGKKTRARVDGIQYQVDINNLTQREVDALPLNGIGLVDLTFDEPLVLDKYQENPVTGGLIFIDRLTNVTVGAGMVREPNTEEAGVASEFSAFELELNALVRKHFPHWGRARFAGREIMAQHDENVVWHAHPVTAQQREQLHGHRGVVLWFTGLSGSGKSTVAGALEEALHQLGVSTYLLDGDNVRHGLCSDLGFTDGDRKENIRRVGEVAKLMVDAGLVVLTAFISPHRAERQMVRERVGSDRFFEIFVDTSLATCEARDPKGLYKKARAGELRNFTGIDSAYESPEQPAIHLDGEQLVTNLVAQLLDLLRQSDIIRS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway.2. The protein design should consider the endogenous proteins and enzymes present in the sulfite from sulfate: step 2/3, Sulfur metabolism pathway to avoid interference with native metabolic processes.3. The Phosphoserine intermediate of the protein must be engineered to enable effective catalysis of adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate   + ADP + H(+).4. The designed protein have Tr-type G domains with specific sequence motifs that are crucial for its activity.5. The protein should be able to bind ATP, GTP ligand in a variety of conditions6. The designed protein must possess sulfate adenylyltransferase (ATP) activity, GTP binding, ATP binding, adenylylsulfate kinase activity to support sulfate assimilation, phosphorylation, hydrogen sulfide biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "MDLLRLSTAGSVDDGKSTLIGRLLYDTKSIFEDQLEAIERASRQRGEGYVNLALLTDGLRAEREQNITIDVAYRYFATPRRKFIIADTPGHVQYTRNMVTGASTAELAIVLVDARKGVLTQSRRHGFIASLLGIPHLVICVNKMDLVDYSEEVFEEIRDEYTGFASKLGVKDLTFLPISALRGDNVAAKSENTPWYDGSTLLHHLETVPVGSSLRFPVQYVNRPNMDFRGYAGRIASGVVKKGDEILVLPSQRRSKVRSIVTFDGERSHAFAPESVTVTLEDEIDISRGDMLVHPAQRPEASDQFEATIVWMDEAALKPGRGYWLKLGSQMVTATVQPPKHEVDVNSFEHLAAKTLELNSIGVAEIATDRPITFEPYESSRQLGGFILIDKFTNATVGAGMLHFALRRAQNVHWQATDVTHEHHAALKNQQPAVVWFTGLSGAGKSTIANLVEKKLHSMGKHTYLLDGDNVRHGLNRDLGFTDADRVENIRRVGEVAKLMVDAGLIVVTAFISPFRAERDMVRKMLPEGEFLEVFVDTPLAEAERRDVKGLYAKARRGELANFTGIDSPYEAPETPEIRIDTTNMDVDAAAERIVAALRERGLL",
    "response#2": "MDLLRLSTAGSVDDGKSTLIGRLLYDTKSIFEDQLEAIERASRQRGEGYVNLALLTDGLRAEREQNITIDVAYRYFATPRRKFIIADTPGHVQYTRNMVTGASTAELAIVLVDARKGVLTQSRRHGFIASLLGIPHLVICVNKMDLVDYSEEVFEEIRDEYTGFASKLGVKDLTFLPISALRGDNVAAKSENTPWYDGSTLLHHLETVPVGSSRGVLRFITCGSVDDGKSTLIGRLLFDSKLLFEDQIRILENESKKYGTAGEGIDFALLLDGLEAEREQGITIDVAYRFFNTEKRKFIVADTPGHEQFTRNMATGASTADLAIILIDARKGILPQTRRHTYIAKMMGIRHIVLAINKMDLAEYDERRFAEIAREYMSDTRGLEFNTLTTIPISARHGDNIFSRSDNTRWYSGPTLLEHLESVDAATEGFRFPVQYVNRPNLNFRGFCGTVASGVIRKGDEVIVLPSNKTSRIKSIVTYDEELELAHAEMAVTLTLEDEIDVSRGDILAAPGEVSDQFQATMLWMSDSAMLPGRSYVMKMGARECQMQVTEERYRIDVNTRAHEAAKTLGLNEIGVVNIALDREVAFDPYNDNRRMGGFIVIDRMTNETVGLGLEASDQFEATIVWMDEAALKPGRGYWLKLGSQMVTATVQPPKHEVDVNSFEHLAAKTLELNSIGVAEIATDRPITFEPYESSRQLGGFILIDKFTNATVGAGMLNFSLRRAENIHWQATDIDRDGRAAIKGQWPAILWFTGISGSGKSTIANLLEKKLFAM",
    "response#3": "TMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALENDSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADCPGHEQYTRNMVTGASTADLAVILIDARKGVLVQTRRHSYLCHLLGIRNIVLAVNKMDLIDYDQARYDAIVAEYETFAKGIGIESFTAMPISGFKGDNIEFEATLAWMDERSSLEVGTPYIIQHTTRRTQAMVDDVLYQLDVNSLHRNDSETLALNEIGRVKITTASELFFDPYERNRSTGGFILIDPNDFRTVGAGMIQSADQFQARIVWMDADALLPGRAHWMKIGTRTISATIQPPKYVVDVNTMAELSAKTLSLNDIGICEVYTDRPVAFEPYADNHDLGGFILIDKVTNATVGAGMLDARVVWMADTSLMPGRQYDLKMGPKTTSAIPELVHYRVDVNTLTHEHADQLGLNDIGLVRWRLNAPVPFDSYQRNPVTGAFIIIDRITNLTVGAGMLDARVVWMADTSLMPGRQYDLKMGPKTTSAIPELVHYRVDVNTLTHEHADQLGLNDIGLVRWRLNAPVPFDSYQRNPVTGAFIIIDRITNLTVGAGMEASDQFEATIVWMDEAALKPGRGYWLKLGSQMVTATVQPPKHEVDVNSFEHLAAKTLELNSIGVAEIATDRPITFEPYESSRQLGGFILIDKFTNATVGAGMLNFSLRRSENVHWQAIDVDKHAHAAQKGQQPAVLWFTGLSGSGKSTIANLVEKKLHALGKHS"
  },
  {
    "reference": "MGIPRRLLLATHNRGKIEEVEQLLGPQGVEIVSARDLGLPEPEETERSFEGNARVKAHAAARATGLPALADDSGLEVAALGGAPGVDTAGWAETPQGRDFVMAMEKVHRLLEERGAPRPRRARFVCVLCLAQPDGGDMLFRGEVEGQLVWPMRGRLGHGFDPVFQPDGHDLTFAEMAPEDKNRISHRARALAAFMRAMQG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a high affinity for substrate.2. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.3. The protein design must be able to perform nucleotide binding, metal ion binding, ribonucleoside triphosphate phosphatase activity in order to contribute to nucleotide metabolic process, purine nucleoside triphosphate catabolic process.4. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MRCV",
    "response#2": "MSLNKRD",
    "response#3": "MLSIRK"
  },
  {
    "reference": "MKYVTPDLCDAYPQLVSVVEPMFQNFGAKSSFGGEIVTVKCFEDNSVVKQQVDQPGHGKVMVVDGGGSKRAALLGDMLAEKAAANGWEGIIIYGCIRDVDVIRQTDLGVQALGTHPMKTDKRGIGDLNADITFGGVTFKPGHYVYADNNGVIVSPEPLSMPQEQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The a divalent metal cation binding site should be stable and able to withstand changes in environmental conditions.2. The designed protein must be able to perform metal ion binding under specific conditions.3. The desired protein should be able to perform H(+) + oxaloacetate = CO2 + pyruvate The designed protein sequence is ",
    "response#1": "IQGTAAVTLGTLIAASRAAGGKLCEQKVVFLGAGSAGCGIAEQIIAQMKSEGLSDEEARARVFMVDRFGLLTDRLPNLLNFQSRLVQKSDSLSGWDSANDSLSLLDVVRNAKPDIMIGVSGQPGLFSEEIVREMHKHCTRPIIMPLSNPTSRVEATPQDIMAWTDGCALVATGSPFSPVSWKDKTYPIAQCNNSYIFPGIGLGVIASGASRVTDSMLMAASRALADCSPLVNDGEGPVLPEVNDIQGVSKIIAMAVGKAL",
    "response#2": "IQGTAAVTLGTLIAASRAAGSQLSEQKIVFLGAGSAGCGIAEQIIAWMRTEGGLSDEQARARVFMVDRFGLLTDNMPNLLSFQSKLVQKRDSLKGWDTQSDSISLLDVVRNAKPDILIGVSGQTGLFTEEIIREMHKHCARPIVMPLSNPTSRVEATPHDILNWTDGAALVATGSPFQPVTVKEKTYPIAQCNNAYIFPGIGLGIISSGALRVTDEMMMAASEALASHSPLVNTGSGLVLPPLTDIQQVSKDIAFAVGKMI",
    "response#3": "IQGTAAVTLGTLIAASRAAGGKLCEQKVVFLGAGSAGCGIAEQIIAQMKSEGLSDEEARARVFMVDRFGLLTDRLPNLLNFQSRLVQKSDSLSGWDSANDSLSLLDVVRNAKPDIMIGVSGQPGLFSEEIVREMHKHCTRPIIMPLSNPTSRVEATPQDIMAWTDGCALVATGSPFSPVSWKDKTYPIAQCNNSYIFPGIGLGVIASGASRVTDSMLMAASRALADCSPLVNDGEGPVLPEVNDIQGVSKIIAMAVGKAD"
  },
  {
    "reference": "MALNPARNVVFVTGNAKKLEEVVQILGGKFPYTLISKKIDLPEYQGESDEISIKKCQEAAMQVQGPVIVEDTCLCFNALGGLPGPKWFLEKLQPEGLHKMLAGFEDKSAYALCTFAYSTGNPKDPVKLFRGKTPGKIVNPRGPRNFGWDPCFQPDGFDLTYAEMPKEVKNSISHRYKALRELADYFIEKNDSTKKARLDEESN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be designed to have high stability and activity towards dITP + H2O = dIMP + diphosphate + H(+).2. The ideal protein would be one that plays a critical role in nucleotide metabolic process, deoxyribonucleoside triphosphate catabolic process through dITP diphosphatase activity, XTP diphosphatase activity, nucleotide binding, metal ion binding, ITP diphosphatase activity within cytoplasm.3. The protein must exhibit the following characteristics: Pyrophosphatase that hydrolyzes the non-canonical purine (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives; Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.; The enzyme does not distinguish between the deoxy- and ribose forms.4. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+), Mn(2+) to enhance enzymatic efficiency.5. The protein should be able to bind Mg(2+), ITP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MKIVFATKNEGKVREIRELLKDHEILTLKDAKLNLPDVPETGNTFEENAKLKAVTISDITGMISLADDSGLEVDSLDGAPGVHTARYAGENATDEENNAKLLMALGDVPVEKRTARFRCVIALALPGDETILAEGRCEGRITTEPAGTGGFGYDPLFIPDGYDRTFGELGEEVKNRISHRGRALTQMREILSVK",
    "response#2": "MVKIGNKEIKNATLIAANKFKGPLDLLLHLIGQYEIDIYDVPIAKVIEQYLAYVQTMQELNLEVAGDYLLMAATLLAIKSKLLLPKPQEDDDDLEVDPRQALVEQLVEYERFKQAAEDLDEREVERAQVFSRQPPELMYAPEFQLDLSDVDLIDFIKQAMTRLVARKPKQVTLQDITIDQYTVEQKMQAVLDVLRQQQHLSLTELFQTDHSRVEIIVTFLAVLELVKQQLVQVKQDVAFSEIYLSKKNV",
    "response#3": "MKIIFATNNQHKLEEVRRILGDNFELISLKDINCHTDIPETADTLEGNATLKAEYIYQKYHVNCFADDTGLEVEALNNEPGVYSARYAGENRDAQANMDKVLTNLQGQTNRSARFRTVISLILDGQEHLFEGIVNGSITLEKTGNSGFGYDPVFVPDGYDITFAEMDMEEKNKISHRGKAVQKLVAYLKSL"
  },
  {
    "reference": "MAIISIEKAIQEIKKGKMLIIVDDESRENEGDLFIPAEKISPKGIAFMATYGRGLICLSMTGERLDQLEIPLMVENNTSSFQTAFCVSIEAKHKTTTGISAYDRAATILTAIDPKTKPSDIARPGHIFPLRAKEGGVLRRPGQTEASIDLCKLAGLYPAGVICEIMKDDGTMARLPDLKKFSAKHKINIVLIADLIQYRLKRETLVKRVAQPKLPTKYGEFRIIAYETELSDKTHIALVMGKWKKGEEVLVRMHSECLTGDALFSLRCDCGAQLECAMKKIAKEKKGAIVYLRQEGRGIGLLNKIKAYELQDRGMDTVEANEALGFKPDERDYGVGAQILRDLGITKARLLTNNPKKLAALSGFGIEIVGCESIEVPPNPVNLKYLKTKKEKMGHRLKKV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must have high specificity towards magnesium ion binding. The designed protein sequence is ",
    "response#1": "RVATATIPTRYGSEFKLIVYENDVDDMKHVALVKGEIEPEDEVLVRVHSECLTGDVFGSERCDCGEQLHTAMMMVEEAGKGVIVYMHQEGRGIGLINKIKAYELQEHGKDTVEANIALGFKADLRDYGIGAQILVDLGVRKMRLMTNNPKKIVGLEGYGMTVTGRVAT",
    "response#2": "VTGTNGKTSCVQMLAQALTLLGHRAATIGTLGSGLHGSIERGERTTPDALSMQALLAGFREAGASHVAMEVSSHALDQGRVEGVDFDLAAFTNLTRDHLDYHGSMQAYGEAKARLFDWPGLKAAAINVDDAFGRRLAERVSEDVKCLRVSVGGVQAEVTATDVEAHGDGLHFVLHTPWGDAAVRSPLLGRFNVANLLLVAAP",
    "response#3": "IDISLSGKGQEVGGLHPVTRTMERIETFFRGIGFDVASGPEIEDDYHNFEALNIPAHHPARAMQDTFYFNPSTVLRTHTSPVQVRTMETTQPPIRIICPGRVYRCDSDQTHTPMFHQVEGLLIDENISFADLKGILQQFLNVFFEDDLKVRFRPSYFPFTEPSIEVDIMRTNSKGEESWLEVLGCGMVHPKVLEMSGIDSEKYTGFAFGMGVERFAMLRYKVDDLRMFFENDLRFLKQV"
  },
  {
    "reference": "MQRIVLASSNRGKLAEFNALLADSHFEVVPQAELGVEDAEETGLTFAENALLKARHASSKTGLPALADDSGLCMPHLRGAPGLYSARYSGGHGDSAANNAKLLRELDGVVPEQRGAFFICVLALLWHADDPAPLIAEGRWHGRVLEAPRGTHGFGYDPLFLPHEQLLSAAELEPSVKNRLSHRAQALAQLHARIAELHV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have ribonucleoside triphosphate phosphatase activity, ITP diphosphatase activity, metal ion binding to facilitate nucleotide metabolic process.2. The protein should be designed to have high stability and activity towards dITP + H2O = dIMP + diphosphate + H(+).3. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.4. The designed protein should have a high specificity for Mg(2+), substrate binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MTEKLVIASNNPGKLREFAAM",
    "response#2": "MSVLSGK",
    "response#3": "MDTIVIATHNQGKVKEIKAMF"
  },
  {
    "reference": "MNKKKSNMPNMPKFNLNWLYITLFIGLVIIWVANDKGSASKDITYTEFQEYVEKGFVTKIHGYDDNTVDVYLKPEHIKDVFQDSTRVGRNPVLLTRVPSMDKLSEYVDAQTELGNFTGKADYNNETKNYFYMFFLNILPFVFFIGLWIFLMRRMSSRGGGAGNVFNVGKSKAQLFEKGGEINVTFKDVAGLSEAKQEIEEIVEFLKQPEKYTDLGGKIPKGALLIGPPGTGKTLLAKAVAGEASVPFFSLAGSDFVEMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAIGRARGRNPSMSGNDERENTLNQLLTEMDGFGSNSGVIILAATNRVDVLDKALLRAGRFDRQIHVDLPDLNERREIFGVHLRPIKMDDNLDIDLLARQTPGFSGADIANVCNEAALIAARKEKKIVQRQDFSDAIDRIVGGLEKKTKIMTKSERKTIAIHEAGHASLSWMLEHANPLIKVTIVPRGRSLGAAWYLPEERQITTKEQMLDEMCALLGGRAAEELFVGKVSSGAMNDLERVTKQAYGMIAYLGMSDELPNLCYYNTNQEYSFNRPYSENTAELIDLEVKRMINEQYERAKRILEKHKEEHNLLAEELTLKEVIHAEDVEKIFGKRPWVSRSEEIMQLEQEAIEEKKEAEKFEKKEKKENETSSDLDQTLKQENNKDIDHSIS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins.2. The protein contains novel AAA+ ATPase domains that confer a unique function or activity.3. The protein should have a high affinity for ATP.4. The designed protein must possess ATP hydrolysis activity to support protein catabolic process under certain conditions. The designed protein sequence is ",
    "response#1": "LLVGPPGTGKTLLAKAVAGEAQVPFFSLSGSDFVEMFVGVGASRVRDLFKQAKEKAPAIIFIDEIDAIGRARGKGAAQGSNDERENTLNQLLTEMDGFGSNSGIIILAATNRAEILDRALLRPGRFDRQISVLFVESLTGFGKITP",
    "response#2": "IPRGVLLYGPPGTGKTLLARAIAGEAGVPFYAMAGSDFVEMFVGLGASRVRDLFDEAKKNAPAIIFIDEIDAVGRKRGSGMSGGHDEREQTLNQLLVEMDGFDNDTNLIIIAATNRPDVLDPALLRPGRFDRQVGVAAPLHFTPDVDPSVIARGTPGFSGADLANLVNEAALFAARRSLRVVGPQQFDVDIMSIARGTPGFSGADLENLVNEAALQAAKLGRDQVLMDDFDAXVGXPIPRGTLMVGPPGTGKTLLAKAIAGEAKVPFFSISGSDFVEMFVGVGASRVRDMFDQAKKQTPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFDGNEGIIVIASTNRPDVLDPALLRPGRFDRQVVVSLPDYARQHGIL",
    "response#3": "IPRGVLLYGPPGTGKTLLARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFKQAKENSPCIIFIDEIDAVGRARGSGMGGGHDEREQTLNQLLVEMDGFGDRGSVILMAATNRPDVLDPALLRPGRFDRQIPVTAPTP"
  },
  {
    "reference": "MTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTFWPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit ribonuclease activity.2. The protein must exhibit the following characteristics: Upon expression in M.smegmatis inhibits colony formation; Its toxic effect is neutralized by coexpression with cognate antitoxin VapB39..3. The designed protein should have a high specificity for Mg(2+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "LLPQDAKTAAATSRIAQAFPGTGSNAIAYLVVEGGSTLEPQDQPYYDAAVGALRADTRHVGSVLDWWSDPVTAPLGTSPDGRSATAMVWLRGEAGTTQAAESLDAVRSVLRQLPPSEGLRASIVVPAITNDMPMQITAWQSATIVTVAAVIAVLLLLRARLSVRAAAIVLLTADLSLAVAWPLAAVVRGHDWGTDSVFSWTLAAVLTIGTITAATMLAARLGSDAGHSAAPTYRDSLPAFALPGACVAIFTGPLLLARTPALHGVGTAGLGVFVALAASLTVLPALIALAGASRQLPAPTTGAGWTGL",
    "response#2": "LLPQDAKTAAATSRIAQAFPGTGSNAIAYLVVEGGSTLEPQDQPYYDAAVGALRADTRHVGSVLDWWSDPVTAPLGTSPDGRSATAMVWLRGEAGTTQAAESLDAVRSVLRQLPPSEGLRASIVVPAITNDMPMQITAWQSATIVTVAAVIAVLLLLRARLSVRAAAIVLLTADLSLAVAWPLAAVVRGHDWGTDSVFSWTLAAVLTIGTITAATMLAARLGSDAGHSAAPTYRDSLPAFALPGACVAIFTGPLLLARTPALHGVGTAGLGVFVALAASLTVLPALIALAGASRQLPAPTTGAGWTGL",
    "response#3": "LLPQDAKTAAATSRIAQAFPGTGSNAIAYLVVEGGSTLEPQDQPYYDAAVGALRADTRHVGSVLDWWSDPVTAPLGTSPDGRSATAMVWLRGEAGTTQAAESLDAVRSVLRQLPPSEGLRASIVVPAITNDMPMQITAWQSATIVTVAAVIAVLLLLRARLSVRAAAIVLLTADLSLAVAWPLAAVVRGHDWGTDSVFSWTLAAVLTIGTITAATMLAARLGSDAGHSAAPTYRDSLPAFALPGACVAIFTGPLLLARTPALHGVGTAGLGVFVALAASLTVLPALIALAGASRQLPAPTTGAGWTGL"
  },
  {
    "reference": "MALSLDDTCLWIRAAAPGGRLVSDSRQVGKGDVFFAYPGEAVDGRAYIAAAIAAGAAAIVFEENGYAWDAAHDVAHLAVAGLKKNAGPIAHGVLEMPDAGMFTVAVTGTNGKTSCAVWAGQALARLGETTAVIGTLGVGLFKGKAEPSFDATGYTTPDAVLLAQKLADMRAQGATALAIEASSIGLVQDRAAGMHFDVAIFTNLTRDHLDFHGDMDSYEAAKRTLFEWEGLRAAIVNLDDPAGQRLVAHLRANVAELAVTGYTLKDEAAQPAIDGVGILRASNMRSRHAGTEFHLESPQGAANLKTQLVGHFNVSNALAVLGALLAKGVALKDAVGAIDALAPAPGRMQQIGGQDAPMVVIDYAHTPDALEKTLGALRQVALERGGQLWCVFGCGGDRDPGKRPQMGAIAQAADHVLVTSDNPRSEEPSTIIAQIVAGMQQRADNQDQAVEDRANAILLAVKNAGKQDVILLAGKGHEPYQEIKGKKMPFSDADHATLALSARLTMMRSH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should contain one or more Mur ligase N-terminal catalytic, Mur ligase C-terminal domains that are essential for its function.2. The protein design must be focused on enhancing the efficiency of regulation of cell shape. The designed protein sequence is ",
    "response#1": "VTGTSGKTTTSYLLEAGLLHAGYSVGLIGTTGTRINREPVPTSLTTPEAPTLQELFARMLSEGVTHVVMEVSSHALELGRVRGTRFDVGGFSNLSQDHLDFHPTMEDYFEAKAALFDGPQAAERAVICVDDDWGKRMAKRAAALPLTRVATQGEADITARQLSTEATGAQQVDLTIAASGTSYEFRLPLPGEFNIANAALATGVTGTNGKTTVATLLYNLFRHMGYPAGLLSTVANYVNDEKYATTHTTLDPISLNGLLSQMVEAGCEYAFMEVSSHAIHQKRTYGLHFVGGVYTNLTQDHLDYHKNMLEYRDVKKSFFDSLPKEAFALVNSDDRNGAIMLQNTKARSKTYSVRGMGDFKARVFEKHFDGSDVEFNGKALSVRFVGLFNVYNLLAVYGAY",
    "response#2": "VVGTNGKTTITYILESIFAAAGFRCGVLGTVNYRYPGIRMAAPNTTPESMDLQRILRDMAESGVTHLVAEVSSHAIDLRRVDDCRFDLGIFTNLTQDHLDYHKTMENYYQAKKRFFSEVLPGGRKLRRHSMVVNVDDPWGKRLVRETGAGNRPLTFGIEENADVRPSCFDLSLDGIKAVIEVGPASFEMASPLIGKFNLSNVLAAVSAL",
    "response#3": "VTGTNGKTTTANMLSAILYEGGYTSCVIGTEGADFGGETIKTGYTTPPPDVLAPLLKRAADENIDFVVMEASSHALDQKRLCGLDFEVGIFTNLSRDHLDYHKSTEAYSAAKAGLFRKCRHSVLNFDDPHAYEMAWAANDEIWYYSAKDFDVDFYADNIVHTEWGVQFDFCSVGAREHISSPLFGSYSVLDMLAAASAL"
  },
  {
    "reference": "MATQNPNQPVTYPIFTVRWLAVHTLAVPSVFFVGAIAAMQFIQR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that This b-type cytochrome is tightly associated with the reaction center of photosystem II (PSII); PSII is a light-driven water:plastoquinone oxidoreductase that uses light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation2. The protein can be expressed in plasma membrane-derived thylakoid membrane and perform identical protein binding, heme binding without triggering undesirable cellular responses.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.4. The heme binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "YPVFAQQGYSNPRAANGKLACANCHLNQKAIEIEAPQGVLPNSVFEIEIKVPYDVNRQQISADGKPADLNVGGILILPKGFKLASKTQISPEVKAKNKGVFISPYSTEFDNILVVGPIAGKTHQELIFPVVAPDPENNPDVKYLTYPFYAGGNRG",
    "response#2": "YPVFAQQGYSNPRAANGKLACANCHLNQKAIEIEAPQGVLPNSVFEIEIKVPYDVNRQQISADGKPADLNVGGILILPKGFKLASKTQISPEVKAKNKGVFISPYSTEFDNILVVGPIAGKTHQELIFPVVAPDPENNPDVKYLTYPFYAGGNRG",
    "response#3": "FPIYAQQNYESPREATGRIVCANCHLAKKAVDIEVPQAVLPDTVFEAVVKIPYDTQIKQVLSNGKKGGLNVGAVLILPEGFELAPSDRIPPELKEKISNIYFQPYSPEKKNILVVGPLPGNKYSELVFPILSPDPATNKKASFLKYPIYLGGNRG"
  },
  {
    "reference": "MIKKNNNSNNNNNNPIIIKDGTNCFFKEIKDVLKFSRNWNLIILVSTIKLLLIPSYLSTDFEVHRNWLAITSSLPISKWYFENTSEWTLDYPPFFGWFEYFLSKFAYYIDSEMLVIDNLGYKSTSTILFQRFSVIISDSLFILSTLLLSNYIYNIIIKKNNNKNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNLHWYHDKSFIISSIIILNPGLLMVDHIHFQYNGFLKGILILSIYFLVRGNILIGSILFSVLLNFKHIYMYMAPAFFVYLLKYYCLKSNLNDNTTSKVNHSKQQQQQEFTIFGIKIFNLIKLGISVLSIFLISLGPFFYMGQIQQLISRLFPFGRGLSHAYWAPNFWSIYNFLDRVLLFNGLYKKIPFFKNFIIPDQVTGNLTSGLVGSETQSHILLPKITPQITLLITILFLIPSIYSILKSKSWKHFILGICQSSFTFFMFGWHVHEKAIIMITIPMGFLCLVNNKFSKLYFLLSTIGHYSLFPLLFQVEEIPTRILILVTYTLLVYLSFISSSSNNNNNNNNNNNNSNNNNSKQRKCSFSLGLYWFEKFYLFGIILLEIFNVFIHPVFLAHIERFSFISLMITSVYTSVGIIYCYLFTFKLIFKNEY",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. The desired protein should be able to perform a dolichyl beta-D-glucosyl phosphate + alpha-D-Glc-(1->3)-   alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-   Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-   (1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-   alpha-D-GlcNAc-diphosphodolichol = a dolichyl phosphate + alpha-D-   Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-   alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-   Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-   beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol + H(+) The designed protein sequence is ",
    "response#1": "MPKVVAFFHPYCNAGGGGERVLWCALRSLQKRYKDAIYVIYTGDKDASEEQILNGAAARFNIKLSHPVRFIFLEKRGLVEASCYPRFTLLGQSLGSVVLGWEALTKCVPDIYIDSMGYAFTLPLFKYLGGCHVGCYVHYPTISMDMLSVVRSQHARFNNAAFISNNPVLSRLKLIYYYLFALFYGWVGSCSDVIMVNSTWTFSHILDLYDADDKFPDQNKINQHKENEEHSVVSIGQFRPEKDHPLQIRAFAALLEKKTAEQRAKLKLILIGGCRNDEDELRVSELKKLSSELGIPVEFKVNVPFEELKKHLSEATIGLHTMWNEHFGIGIVECMAAGTIILAHNSGGPKLDIVVPHEEQQTGFLADSVDSYAAAMDHILSLTPEQRLSIRQNALNFQNSANDM",
    "response#2": "MDGKVVAFFHPYCNAGGGGERVLWCALRSLQKRYKDAIYVIYTGDKDASEEQILNGAAARFNIKLSHPVRFIFLEKRGLVEASCYPRFTLLGQSLGSVVLGWEALTKCVPDIYIDSMGYAFTLPLFKYLGGCHVGCYVHYPTISMDMLSVVRSQHARFNNAAFISNNPVLSRLKLIYYYLFALFYGWVGSCSDVIMVNSTWTFSHILDLNQHKENEEHSVVSIGQFRPEKDHPLQIRAFAALLEKKTAEQRAKLKLILIGGCRNDEDELRVSELKKLSSELGIPVEFKVNVPFEELKKHLSEATIGLHTMWNEHFGIGIVECMAAGTIILAHNSGGPKLDIVVPHEEQQTGFLADSVDSYAAAMDHILSLTPEQRLSIRQNACSHSSQ",
    "response#3": "MAAPMKVVAFFHPYCNAGGGGERVLWCALRSLQKRYKDAIYVIYTGDKDASEEQILNGAAARFNIKLSHPVRFIFLEKRGLVEASCYPRFTLLGQSLGSVVLGWEALTKCVPDIYIDSMGYAFTLPLFKYLGGCHVGCYVHYPTISMDMLSVVRSQHARFNNAAFISNNPVLSRLKLIYYYLFALFYGWVGSCSDVIMVNSTWTFSHILDLLLQQLPSFYTPNQHKENEEHSVVSIGQFRPEKDHPLQIRAFAALLEKKTAEQRAKLKLILIGGCRNDEDELRVSELKKLSSELGIPVEFKVNVPFEELKKHLSEATIGLHTMWNEHFGIGIVECMAAGTIILAHNSGGPKLDIVVPHEEQQTGFLADSVDSYAAAMDHILSLTPEQRLSIRQNAWNYPELTNSEVPNQHKENEEHSVVSIGQFRPEKDHPLQIRAFAALLEKKTAEQRAKLKLILIGGCRNDEDELRVSELKKLSSELGIPVEFKVNVPFEELKKHLSEATIGLHTMWNEHFGIGIVECMAAGTIILAHNSGGPKLDIVVPHEEQQTGFLADSVDSYAAAMDHILSLTPEQRLSIRQNAWNK"
  },
  {
    "reference": "MSHRPLACVILAAGKGTRMKSALPKVLHPLGGKPMVNHVVDTALSLSPDKVVVVVGPGMDNVAAAVAPHPTVVQVNQRGTGDAVAAARGELEGFQGDVIVLYGDTPLIRAEVLRAMVTARAEKDAAVVVMGMRPADPGAYGRLVVGADGGLDAIVEFLDATPEQRAITLCNAGFMAFDGARLWTLVDGIGNDNAKGEYYLTDAVAVARSKGWACAVVEGPVEDAIGVNSRAELAGIERIFQDRLRKAAMDNGATLLDPATTYFSADTKLGRDVVVGQNVVFGPGVEVGDNVTIKPFCHFEGVRMAAGAEIGPFARLRPGTVVEEGCHIGNFVELKNTTLGKGSKANHLAYLGDSDIGSGSNIGAGTITCNYDGFLKHRTTIGDGVFVGSNSTLVAPVTLEDGAYVAAATTVTRTVTTNSMAISRVPQEVKEGAAARFRDRKKAEKAAKAAKKG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have a high affinity for UDP-N-acetyl-alpha-D-glucosamine, Mg(2+).2. A protein with UDP-N-acetylglucosamine diphosphorylase activity, magnesium ion binding functions is required to enable cell morphogenesis.3. We are looking for a protein with broad substrate specificity for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate. The designed protein sequence is ",
    "response#1": "AIILAAGKGTRMKSDLHKVLHPIAGRPMLLHLIDSFDAAGAAKRVVVIGSAREQIEAAVAERNVDLALQSEQLGTAHATQMAKDALTGFDGTVIVAFGDTPFLKPETITRLAGALEGEGAPRILREPNGYGRVVREGERVVGIVEQKDATPEQLAIHEVNTSVYVFDAAVLREAIGRLGNDNAQGEYYLTDVIGLARADGLRVGAHVVEDVALTEGANDRAQLAALRAELNRRILERWMLDGVSIVDPANTYLDMDVTIGRDTLVEPGCVITGATVIGEGCHIKPHCTIEDSRLGDETVIGPSAHLRPGTELGRGCRIGNFVEVKNSKLADGVKADHLAYVGDADVGEGSSFGCGAITVNYDWDQKHRTTVGRDVKVGCNANLVAPVTLADGAVVAAGSTITTSVPEQSLAVARAKQRNIEGWVARKRPGSASAQAAAKAQEEG",
    "response#2": "ELMAAGVSMQAPETVFLAADTDIAADVEIEPFVVFGPGVTIASGARIRAHSHIEGATIGEGCEIGPFARLRPGTVLGKGAKIGNFVETKKAVLGDGAKANHLTYLGDVTVGAKANIGAGTITCNYDGFFKYQTVIGAGAFIGSNSALVAPVTIGDGAIVAAGSTVTSDVSADALALVRPARHFDNIIFVDD",
    "response#3": "VVILAAGQGTRMKSRRSKVLHEIAGRPMVGFPVALARELSASEPVVVVGRDAEDVQAALAGQAKFVLQAEQNGTGHAVLCAQDALADARDAILILYGDTPLLRLESLRRMAEMLAADDADLVMLTSPEAEGYGRIVRDAGGAVLAIVEHKDASDAQRQIREVNTGILAVPGKRLAGWLARLGNANAQGEYYLTDIVAMAVADGVAVETAQPADATEVLGANDPWQLSVLERAFQLRQAEALMLAGVSIVDPANTYLDMDVTIGRDTLVEPGCVITGATVIGEGCHIKPHCTIEDSRLGDETVIGPSAHLRPGTELGRGCRIGNFVEVKNSKLADGVKADHLAYVGDADVGEGSSFGCGAITVNYDWDQKHRTTVGRDVKVGCNANLVAPVTLADGAVVAAGSTITTSVPEQSLAVARAKQVSIAGWKRPVKA"
  },
  {
    "reference": "MRIGILTGGGDCPGLNAVLRAVVRRSVKEYGSEVVGFLEGFRGVMNNMVMPLTMNSTGGLIHRGGTILRTSRTNPFASEDGPQRIKDNMERNRLDALIVVGGEDTCGVANRLYQEHKLNLVCVPKTIDNDLNATDVTFGFDTAVNIVAEALDRLHTTAESHNRVLVCEVMGRNAGWIACYGGIAGGADFILVPEKPINLTEVISAIKQRHARGRDFSIVVVAEGARFDDETKNPVKEEDANGRVKLGGIGGILAKHIEGETGFETRATTLGHIQRGGSPTAFDRVLATRYGVMAADLVHQKKFGQMVALHGNTLTDVPIQDAVNSLKRLDMDIYKVAEVFFG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis2. The designed protein should contain one or more Phosphofructokinase domains that are essential for its function.3. The protein must have high specificity towards 6-phosphofructokinase activity, metal ion binding, ATP binding.4. The Proton acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.5. The protein should be optimized for activity in Carbohydrate degradation, D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 3/4 pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MIVAQSGGPSPVINNSLRGLVETARDLPEIGTIYAGWHGIEGVLKEELLNLSGQSPEEIALLRVTPAAGSVGTCRYKLKEHQNEDFDRIIEVFKAHNVGYFCYIGGNDSMDTANKVAQMATERGVDVVGIGVPKTIDNDVGDSEFKLIDHTPGYGSTARYWLSMVQMANEENRGSCPADPVLVLQAMGRKIGFIPAAARLADPQRKIPMQIYLAENPVSIEQIHTQINDQLKKDGRLIVVVSEGLSLGDIGETKDSFGHTQFSSSQMTVAQLLVNELNQRGLAVKGAARANVPGTDQRYAQSGGVTAVINASAAAVIEAARGRPDVVGTVLAARHGILGVLAEDLVDTAGLAPDALQALAHTPGGAFGSCRFDLDPPQDNPAQYDRLFAVLAAHDIRYFLYNGGNGSMDTVAKISAAARARGYPLVCVGIPKTVDNDLEGSDCSPGFGSAAKYAATAMLEAGLDLAAMSSRVGRAFVLEVMGRNAGWIAAATALAARGPDEPPHIILMPEVPFDEDVFLDAVRATVERLGYCAITVSEGIRRGDGSLLMEQDHDSKGHVQLGGAGQCIARLIQARLGYKHHWAIPDYLQRAAGHLVSATDYAHAMAVGAAAVDLVLLGKLGRITAQSGGVTSVINASACGVIETARKHPDRIGNVYAGRNGIIGALTEELIDTGAEPAEAIAALRSTPAGAFGSCRYKLKSLEENRREYERLIEVFKAHGIGYFFYNGGGDSADTCFKVSQLSQSLGYPLQAIHVPKTIDNDLPLTDCCPGFGSVAKYVAVSTLEASFDVRSMAATSTKVFVLEVMGRHAGWIAAAGGLVADHGIPVVVLFPEIEFDKARFLARVDELVKQHGYCSVVVSEGCHYPDGTFLAEQGTRDAFGHAQLGGAAPVVAQMVKEALGHKFHWAVADYLQRAARHIASATDVKQAYELGQRAVE",
    "response#2": "MIVAQSGGPSPVINNSLRGLVETARDLPEIGTIYAGWHGIEGVLKEELLNLSGQSPEEIALLRVTPAAGSVGTCRYKLKEHQNEDFDRIIEVFKAHNVGYFCYIGGNDSMDTANKVAQMATERGVDVVGIGVPKTIDNDVGDSEFKLIDHTPGYGSTARYWLSMVQMANEENRGSCPADPVLVLQAMGRKIGFIPAAARLADPQRKIPMQIYLAENPVSIEQIHTQINDQLKKDGRLIVVVSEGLSLGDIGETKDSFGHTQFSSSQMTVAQLLVNELNQRGLAVKGAARANVPGTDQRRVGVLTGGGDCPGLNAVIRAVVRKGTQEYGHTFAGFRDGWRGPLRGDVRPLDIAAVRGILPRGGTVLGSSRTNPFQEEDGVRRIKETLAKEEVEALIAIGGEDTLGVAARLWEEHGIPCVGVPKTIDNDLSATDYTFGFDTAVGVATEAIDRLHTTAESHMRVLVVEVMGRHAGWIALHSGLAGGANVILIPEQRFDVEQVCAWIESRFRVRYAPIVVVAEGAMPQDGQMVLKDGSTDAFGHVRLSGVGEWLAKEIEARTGKEARTTVLGHTQRGGTPSAFDRWLATRFGLHAVDALINGHQATRVGVLTGGGDCPGLNAVIRAIVRRGVPEYGYDFLGFRDGWRGPLEAETRPLDIPAVRGLLPRGGTILGSSRTNPFKIDDGVTRIRKNLQSLGVDALIAIGGEDTLGVASELHEKGVKVIGVPKTIDNDLSGTDYTFGFDTAVTIATEAIDRLHTTAESHHRALIVEVMGRHAGWIALHSGMAGGANAILIPEVRFDIDQVCSWVESRFRLDYAPIIVVSEGAMPQDGENVLVAGGKDEFGHARLGGIGDWLAHEIESRTGKEARTTVLGHVQRGGTPSARDRWLATRFGLHAID",
    "response#3": "MIVAQSGGPSPVINNSLRGLVETARDLPEIGTIYAGWHGIEGVLKEELLNLSGQSPEEIALLRVTPAAGSVGTCRYKLKEHQNEDFDRIIEVFKAHNVGYFCYIGGNDSMDTANKVAQMATERGVDVVGIGVPKTIDNDVGDSEFKLIDHTPGYGSTARYWLSMVQMANEENRGSCPADPVLVLQAMGRKIGFIPAAARLADPQRKIPMQIYLAENPVSIEQIHTQINDQLKKDGRLIVVVSEGLSLGDIGETKDSFGHTQFSSSQMTVAQLLVNELNQRGLAVKGAARANVPGTDQRRVGVLTGGGDCPGLNAVIRAVVRKGTQEYGHTFAGFRDGWRGPLRGDVRPLDIAAVRGILPRGGTVLGSSRTNPFQEEDGVRRIKETLAKEEVEALIAIGGEDTLGVAARLWEEHGIPCVGVPKTIDNDLSATDYTFGFDTAVGVATEAIDRLHTTAESHMRVLVVEVMGRHAGWIALHSGLAGGANVILIPEQRFDVEQVCAWIESRFRVRYAPIVVVAEGAMPQDGQMVLKDGSTDAFGHVRLSGVGEWLAKEIEARTGKEARTTVLGHTQRGGTPSAFDRWLATRFGLHAVDALAGIPAPVERLAALRPEQWRRYGPTFALFEGREPVTVPVGRWPG"
  },
  {
    "reference": "MIHKNWAELIKPTQLEVKPGNDPARQATLVAEPLERGFGLTLGNALRRILMSSLQGAAITSVQIDNVLHEFSSVAGVREDVTDIILNLKQVSLRMEVEGPKRLSINAKGPAVVTAGDIAETAGIEVLNREHVICHLDDGADLFMELTVNTGKGYVSAEKNKPEDAPIGLIPIDAIYSPVKKVSYDVQPTREGQVLDYDKLTLKVDTDGSITPEDALAFAARILQDQLSIFVNFDEPESAGRQDEDDGLEFNPLLLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLSGMGLHLGMDVEDWPPDNIEELAKKFEDSF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates..2. The protein should be designed to have high stability and activity towards a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +   RNA(n+1).3. A protein that can perform protein dimerization activity, DNA-directed 5'-3' RNA polymerase activity, DNA binding in DNA-directed RNA polymerase complex, cytoplasm to support DNA-templated transcription. The designed protein sequence is ",
    "response#1": "NLIEIQLKSFKWFLEEGLLDVLKKYSPIKSQIQRPDSRKNEKGFSLEFVRTRIGNPKYNIQECKDKGLTYSVPLYVTVRITDLYSGEIKEEEAFFGYLPYMTDNASFIINGAERVIVNQLVRSPGVYFVDEPTKASSGSLPILVAHFLPVKGAWLEILLNPNKKVLQVRIDRRRKMNLFLLLKTLGYEDDLELLDLFVHDVDANDEYVLLEHVGSIILSDVKTRNELIAERGAVLTEALAKKIAESDVDVIKLPMPIAMSTLKAFHRTYGENIKSEDAYIEIFRKLRPGEIPRINAAKNYLHNLYFSPDRFDFSEVGRHKINSRLRKVYKEYLKEVKKTEVPEDIEYAEESTVLTELDIVLAARHLAQLESHPELLDTKDHLGNKRVRTVGELMQNEFEKAFVKIHKLLEEKLTIYTSFDKVTVQNLVNVRTRSPSVYYNEKIDKNGKRGIGATVIPNRGAWLEFETDAKDIAYVRIDRTRKLPITVLLRALGFGTDQEITDLLGENEYLRNTLEKDNTENAEKALLEIYERLRPGEPPTVENAKSLLVSRFFDPKRYDLAHVGRYKMNKKLHMKNRLFNQK",
    "response#2": "NLIEIQLKSFKWFLEEGLLDVLKKYSPIKSQIQRPDSRKNEKGFSLEFVRTRIGNPKYNIQECKDKGLTYSVPLYVTVRITDLYSGEIKEEEAFFGYLPYMTDNASFIINGAERVIVNQLVRSPGVYFVDEPTKASSGSLPILVAHFLPVKGAWLEILLNPNKKVLQVRIDRRRKMNLFLLLKTLGYEDDLELLDLFVHDVDANDEYVLLEHVGSIILSDVKTRNELIAERGAVLTEALAKKIAESDVDVIKLPMPIAMSTLKAFHRTYGENIKSEDAYIEIFRKLRPGEIPRINAAKNYLHNLYFSPDRFDFSEVGRHKINSRLRKVYKEYLKEVKKTEVPEDIEYAEESTVLTELDIVLAARHLAQLESHPELLDTKDHLGNKRVRTVGELMQNEFEKAFVKIHKLLEEKLTIYTSFDKVTVQNLVNVRTR",
    "response#3": "NKDSSSKVVSSEFDLIPGVSEDTLEVIANIKNIHLKLGEGEQRKIISFSVSGKDTKVLKASHFERDGVEVFNRDLVIATLSQDVNLDLEFQINYGRGYVSSEQNSKYLEEVNVIALDSIFR"
  },
  {
    "reference": "MPRQEHIRNFSIIAHIDHGKSTLADRILEVTGLVSDREKRDQYLDRMELERERGITIKAQSVRIPYTAKDGRKYVLNLIDTPGHVDFNYEVSRSLAACEGALLVVDASQGVEAQTLANVYLALDHNLEVIPVLNKVDLPSADADRVKHEIEESIGLDCSDAVAVSAKTGLNVDKVLEAIVERLPAPEGNLNAPLKALIFDSWYDSYQGVVVLFRIVDGVLRKGDRIKLFATERSYEVIRLGVFSPEIVDMTELGAGEVGFLCANIKELGDAKVGDTITHVDRPAEVPVEGFKEVQPMVFCGLYPTDSADYEQLKYALEKLQLNDAAFSYEPETSQALGFGYRCGFLGLLHMEIIQERLEREFQVELIATAPSVIYKIDTVDGKKSDIDNPSKLPDPTKIAALYEPYVRMDIHVPNDYVGNVLKLCEEKRGIQKNMGYIASNRVVITYELPFAEIVFDFFDRLKSGTKGYASMDYEFIDYRQSALVKLDILINGEAVDALAVIVHRDKAYHYGRALALKLKRTIPRQMFEVAIQAAIGQKIIARESISALRKNVTAKCYGGDITRKRKLLEKQKEGKKRMKRMGNVELPQEAFLAALQVGDE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein with GTP binding, translation elongation factor activity is needed to enhance positive regulation of translation in a specific context.2. The protein should have a low Km value for GTP + H2O = GDP + H(+) + phosphate.3. For general function, the protein need meet that Binds to ribosomes in a GTP- dependent manner. The designed protein sequence is ",
    "response#1": "NFSIIAHIDHGKSTLSDRIIQICGGLTERELKEQVLDSMDLERERGITIKAQSVTLDYKAEDGQVYQLNFIDTPGHVDFSYEVSRSLYACEGALLVVDAGQGVEAQSVANCYTAVEQGLEVLPVLNKMDLPQADPDRVAHEVEEIIGIDASDACRVSAKSGLGMEAV",
    "response#2": "NFSIIAHIDHGKSTLSDRIIQICGGLTERELKEQVLDSMDLERERGITIKAQSVTLDYKAEDGQVYQLNFIDTPGHVDFSYEVSRSLYACEGALLVVDAGQGVEAQSVANCYTAVEQGLEVLPVLNKMDLPQADPDRVAHEVEEIIGIDASDACRVSAKSGLGMEAE",
    "response#3": "NFSIIAHIDHGKSTLSDRIIQICGGLTERELKEQVLDSMDLERERGITIKAQSVTLDYKAEDGQVYQLNFIDTPGHVDFSYEVSRSLYACEGALLVVDAGQGVEAQSVANCYTAVEQGLEVLPVLNKMDLPQADPDRVAHEVEEIIGIDASDACRVSAKSGLGMEAA"
  },
  {
    "reference": "MSLDSEVSVSSSSGRDWFFPSPSFFRSSPSQYGRRFHTNSNTHSAPSSTYPSGIRHRRRVKFSRTPTTSSNEKPQISIVSDKPSAISKNNLNWLSQFGLQFALVTLTIVFLLLLLLRNTHLESQVNKLQGEILRLHACHQLDTLNVSSSTAHKSQDTHPCSCENFKRNLALFLSFMLLLIPLIIFKYIDYVSRSRLSENISEQVSLNKQIAYRVDVFLSVYPYAKPLVLLVATLLLIFLGGLTLFGVTTEDLGHCLWLSWTYVADSGNHASSEGIGPRLVAVSISFGGMLIFAMMLGLVSDAISEKFDSLRKGKSEVVEQNHTLILGWSDKLGSLLNQLAIANESLGGGTIAVMAERDKEDMELDIGKMEFDFKGTSVICRSGSPLILADLKKVSVSKARTIIVLAEDGNADQSDARALRTVLSLTGVKEGLRGHIVVEMSDLDNEVLVKLVGGDLVETVVAHDVIGRLMIQCARQPGLAQIWEDILGFENCEFYIKRWPQLDGMLFEDVLISFPAAIPCGIKVASYGGKIILNPDDSYVLQEGDEVLVIAEDDDTYAPAPLPMVRRGSLPKDFVYPKSPERILFCGWRRDMEDMITVLDASLAPDSELWMFNDVPEKEREKKLIDGGLDISRLENISLVNREGNAVIRRHLESLPLESFDSILILADESVEDSAIQADSRSLATLLLIRDIQARRLPYVAMASQTQGGNFSKGSWIGEMKQASDKTVIISEILDPRTKNLLSMSKISDYVLSNELVSMALAMVAEDR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Also involved in fungal entry into root epidermal cells during the establishment of the arbuscular mycorrhizal symbiosis.; Acts early in a signal transduction chain leading from the perception of Nod factor to the activation of calcium spiking; Closed at negative voltages in presence of magnesium; Involved in perinuclear calcium spiking but not in cytosolic calcium influx; Ion channel with a moderate preference for potassium over sodium and calcium.2. The protein should be optimized for stability and solubility when expressed in nuclear membrane where it will be involved in ion transport.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.4. The protein should exhibit Polar residues compositional bias.5. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "AVIIAGMGFFTDAYDLFCISTVSKLLGRLYYYDPSTKAPGKLPHMANNWVIGVALVGTLSGQLVFGWLGDKLGRKKVYGLTLILMVICALCSGLSLGYSPKSVIGTLCFFRFWLGFGIGGDYPLSATIMSEYANKSTRGAFIAAVFAMQGVGIIFAGLVSMTISKVFLMNFEGKPFNVDEVLSTEPEADYVWRIVLMLGALPALLTYYWRMKMPETGRYTAIIEGNAKQAAIDMGKVLDIEIQAEGDKLAQFKAANEYSLLSNEFFQRHGLHLIGTMSTWFLLDIAFYSQNLTQKDIFPVMGLTSKANTISALREMFETSRAMFVIALFGTFPGYWFTVFFIEKIGRFKIQLVGFFMMSVFMAIIGVKYDYLRNKEHKWTFAALYGLTFFFANFGPNSTTFVLPAELFPTRVRSTCHALSAALGKAGAMISAFGIQQYTQDQDVRKIKTAMLLLAFTNMVGFCCTFLVTETKKNPPGVRGYCIDVFEAAIELLPYPVPRTYILYGDGKRNPSYDNLVNEVVADNFDVAVGDITIVTNRTRYVDFTQPFIESGLVVVAWLYMAVIIAGMGFFTDAYDLFCISTVSKLLGRLYYYDPSTKAPGKLPHMANNWVIGVALVGTLSGQLVFGWLGDKLGRKKVYGLTLILMVICALCSGLSLGYSPKSVIGTLCFFRFWLGFGIGGDYPLSATIMSEYANKSTRGAFIAAVFAMQGVGIIFAGLVSMTISKVFLMNFEGKPFNVDEVLSTEPEADYVWRIVLMLGALPALLTYYWRMKMPETGRYTAIIEGNAKQAAIDMGKVLDIEIQAEGDKLAQFKAANEYSLLSNEFFQRHGLHLIGTMSTWFLLDIAFYSQNLTQKDIFPVMGLTSKANTISALREMFETSRAMFVIALFGTFPGYWFTVFFIEKIGRFKIQLVGFFMMSVFMAIIGVKYDYLRNKEHKWTFAALYGLTFFFANFGPNSTTFVLPAELFPTRVRSTCHALSAALGKAGAMISAFGIQQYTQDQDVRKIKTAMLLLAFTNMVGFCCTFLVTETK",
    "response#2": "RIGVPNRVSYTDYVSKDKNPPGVRGYCIDVFEAAIELLPYPVPRTYILYGDGKRNPSYDNLVNEVVADNFDVAVGDITIVTNRTRYVDFTQPFIESGLVVVAPVKEAKSSPWSFLKPFTIEMWAVTGGFFLFVGAMVWILEHRFNQEFRGPPRRQLITIFWFSFSTMFFSHRENTVSSLGRFVLIIWLFVVLIINSSYTASLTSILTIRQLTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAFGGSSEGFIGRHNFALRDFPTPTADYSFFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFVYPVVSHWFWSPDGWASPFRSADDRLFSTGAIDFAGSGVVHMVGGIAGLWGALIEGPRRGRFEKGGRAIALRGHSASLVVLGTFLLWFGWYGFNPGSFTKILVPYNSGSNYGQWSGIGRTAVNTTLSGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITAGCSVVEPWAAIVCGFMASVVLIGCNKLAELVQYDDPLEAAQLHGGCGAWGLIFVGLFAKEKYLNEVYGATPGRPYGLFMGGGGKLLGAQLVQILVIVGWVSATMGTLFFILKRLNLLRISEQHEMQGMDMTRHGGFAAA",
    "response#3": "FTVSNFDPYKTNIELEGNAFISDGSIHLTNVIPNSAGRASWGGPVRLWDADTGNLAGFTSVFSFEVAPAGPGLIGDGITFFIAPFNSHIPKNSSGGFLGLFNAETALNTYQNRIVAVEFDSFGGNSGGNPWDPAYPHVGIDVNSIASVTTAPWKTGSILTGFNAIAFVNYEPVEKNLSVVVRYPGGNFVNGTSNSVSFIIDLRTVLPEWVRIGFSGATGQLVELHKILSWTFKSSG"
  },
  {
    "reference": "MMKCLFLLCLCLLPIVVFSSTFTSQNLIDLPSESPLPKPVLDTNGKELNPNSSYRIISIGRGALGGDVYLGKSPNSDGPCPDGVFRYNSDVGPSGTFVRFIPLSGGIFEDQLLNIQFNIATVKLCVSYTIWKVGNLNAYFRTMLLETGGTIGQADSSYFKIVKLSNFGYNLLYCPITPPFLCPFCRDDNFCAKVGVVIQNGKRRLALVNENPLDVLFQEV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Protects the plant by inhibiting proteases of invading organisms.; Inhibitor of cathepsin D (aspartic protease); May also inhibit trypsin and chymotrypsin (serine proteases)2. The protein must contain a signal peptide for proper functionality.3. The protein's functional design necessitates the inclusion of a Vacuolar targeting signal motif.4. A protein that contributes to negative regulation of peptidase activity by functioning in vacuole with aspartic-type endopeptidase inhibitor activity, serine-type endopeptidase inhibitor activity. The designed protein sequence is ",
    "response#1": "YLSPDLIRKEMNVSQTSTNSTHLGNSQAIAQFLKEYFSPSDLKIFQYRFGLEPSQVDNIIGPNQNLNPGIETALDIQYIMAMAPDVPTWIVSTGGLHEGQEPFLDWLVDLSSNPKLPLVHSISYGDDESSIGLAYTDRVDTEFKKYAAMGRTIVFSSGDFGVGCNDDCDSFSPGWPASSRFVLAVGGVIKKKDGSIIGDEISGGGFSNYFSRPWYQVDECSSYIEWLNGSLSSFYNQSGRGFPDISSFSENVVILYKDKLMPIGGTSASAPIIAGLLSLINDQRLQKNQSPIGLFNPLLYKIARDHPNSFLDIDFGENNYKCCTNGFKSKSGWDPVTGLGLPNFDELVKYCLN",
    "response#2": "RGVTSYVIDTGVNINHKDFEKRAIWGKTIPLNDEDLDGNGHGTHCAGTIASKHYGVAKNANVVAVKVLRSNGSGTMSDVVKGVEYAAKAHQKEAQEKKKGFKGSTANMSLGGGKSPALDLAVNAAVEVGIHFAVAAGNENQDACNTSPASADKAITVGASTLSDDRAYFSNWGKCVDVFAPGLNILSTYIGSDDATATLSGTSMASPHVAGLLTYFLSLQPGSDSEFFELGQDSLTPQQLKKKLIHYI",
    "response#3": "YLSPDLIRKEMNVSQTSTNSTHLGNSQAIAQFLKEYFSPSDLKIFQYRFGLEPSQVDNIIGPNQNLNPGIETALDIQYIMAMAPDVPTWIVSTGGLHEGQEPFLDWLVDLSSNPKLPLVHSISYGDDESSIGLAYTDRVDTEFKKYAAMGRTIVFSSGDFGVGCNDDCDSFSPGWPASSRFVLAVGGVIKKKDGSIIGDEISGGGFSNYFSRPWYQVDECSSYIEWLNGSLSSFYNQSGRGFPDISSFSENVVILYKDKLMPIGGTSASAPIIAGLLSLINDQRLQKNQSPIGLFNPLLYKIARDHPNSFLDIDFGENNYKCCTNGFKSKSGWDPVTGLGLPNFDELL"
  },
  {
    "reference": "MGWLCTKFLSSVSCLILLWVTGSGGIKVLGDPTCFSDYIRTSTCEWQLDSTVDCSSQLLLDYRLLFEFSENLTCTPKNSADTVCVCQMAIEEPIQADTYWLELWSERGQLWQGSFKPSDNVKPPAPDNLTLHTNVSNALLLMWSNPYPSNNFLHKGLICMVNISREDNPAEFKVYNVTYTEPKLSFPVNTLTSGVRYRARVRVLSQSFPGIWSEWSPSITWYNHFQLPLLQRLPLGVSISCICILLFCLTCYFSIIKIKKIWWDQIPTPARSPLAAIIIQDTKVSLWEKQTRSQESTKSRHWKTCLTKLLPCLLEHRVKKERESPKAAKTKPLQSPEKAGWYPAEVSRTVLWPENVHVSVVRCMELFEAPVQNVEEEEDEMVKGDLSMSPENSGGGFQESQADIMARLTENLFSDLLGAENGGVGQSSMAESSSLLPSESGQASTSWACFPTGPSETTCQVTGQQPPHPDPERATGTACTQVPLVISDNPAYRSFSDFSSPAPNPGELASEQKQAGHLEEGDLLSPVDPHSSGPPMQQAESWEQILHMSVLQHGTAGSTPAPTSGYQEFVQAVKQGASQDAGVPGVGPSGDTGYKAFSSLLSSSGVCTDTAAAGTDSGCGGYKPFQNPVPNQSPNSMPLFTFGLDMELPPSPLNSAPPNSTPECLGLELGLKGGTWLKAPPPSEQVPKPFGDDLGLGIVYSSLTCHLCGHLKQHHSQEEDGQIHVVASPGCGCCYDEKSPSLGNLSGTLESCPGEMSQEANLTLAPRT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The Extracellular, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.3. A protein that contributes to production of molecular mediator involved in inflammatory response, positive regulation of chemokine production, cytokine-mediated signaling pathway by functioning in nucleoplasm, extracellular space with cytokine receptor activity. The designed protein sequence is ",
    "response#1": "GNSLVLWVLVKYESLESLTNIFILNLCLSDLVFACLLPVWISPYHWGWVLGDFLCKLLNMIFSISLYSSIFFLTIMTIHRYLSVVSPLSTLRVPTLRCRVLVTMAVWVASILSSILDTIFHKVLSSGCDYSELTWYLTSVYQHNLFFLLSLGIILFCYVEILRTLFRSRSKRRHRTVKLIFAIVVAYFLSWGPYNFTLFLQTLFRTQIIRSCEAKQQLEYALLICRNLAFSHCCFNPVLYALLSKQFRSAFRRILTRPVLWVGVSMQFIPAEIPRSAFECREQVVSEQTLVQSNICLYIDKRSKNLLGSRDLQSSVTLDLALDPGRLSPRATFQETKNRSLSRVRVLGLKAHCENFNLLLPSCVEDSVTPITLRLNFTLVGKPLLAFRNLRPMLAADAQRYFTASLPS",
    "response#2": "KEALVKSEMNVNMKYQLPNFTAETPIHNVVLPGHHIYLGATNYIYVLNDKDLQKVSEFKTGPVVEHPDCFPCQDCSSKANVSGGVWKDNVNMALLVDTYYDDQLISCGSVNRGTCQRHVLPPDNAADIQSEVHCMFSPLAEEESGQCPDCVVSALGAKVLLSEKDRFINFFVGNTINSSYPPDYSLHSISVRRLKETQDGFKFLTDQSYIDVLPEFRDSYPIKYIHAFESNHFIYFLTVQKETLDAQTFHTRIIRFCSVDSGLHSYMEMPLECILTEKRRKRSTREEVFNILQAAYVSKPGANLAKQIGASPYDDILYGVFAQSKPDSAEPMNRSAVCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEVRSDEYRTEFTTALQRVDLFMGRLNHVLLTSISTFIKGDLTIANLGTSEGRFMQVVLSRTAHFTPHVNFLLDSYPVSPEVIVEHPSNQNGYTLVVTGKKITKIPS",
    "response#3": "DNILVVFILVKYKGLRQAENMSFLNLALSNLGFLLTLPFWAYAASHGEGFDDPLCKILLLLYSIGLYSEAFFNVLLTVQRYKEFFHVRRRFSACRTVAGSIFISVLVWVTATLVTLPELVSYKPQMQSQKYKCFFTGLHFLPADETFWKHFLTLKMNILGFLLPLFAFVYCYVRMRKTLQFRERNYGLFKLVFTIMAVFLLMWGPYNIVLFLSAFNEHFSLHGCGSSYNLNKSVQITRIIAATHCCVNPLLYALRSPELRAALGRALG"
  },
  {
    "reference": "MAFEFDSIEAALADLKAGKALVVVDDENRENEGDVICAAQFATPDLINFMAVEARGLICLAMMGDRLDQLDLPLMVSNKAYEDSNEQTAFTVSIDAALHLGVTTGISADDRARTIQIAINPEAKPNDLRRPGHIFPLRAREGGVLKRAGHTEAGVDLARLAGLYPAAVICEISNPDGSMARLPQLIEYARTHHLKLISIADLISYRLQHERFIHREAIAQLPTQFGQFQVYAYRNLLDNSEHLAIVKGDPATFKDAPILVRVHSECLTGDALGSLRCDCRMQLQAALKMIENAGAGVVVYLRQEGRGIGLVNKLKAYSLQDMGLDTVEANQKLGFSADQRNYGMGAQILNDVGVKQFRLITNNPRKIAGLKGYGLDMVDRVPLLIETTSHNSVYLATKAEKLGHLLLQTYLITVAIQWQDDPQSPTERYERLEKLRHLVKEHDLLLQEEARPVAIALFGRSSLIFHLGLDQPRLAAADWYDHPGHPYVKAIAQILDDITTLQPVQQLEFLVASGHDPMNNLQVQLDRQIFRLDKHSTVETIKPSSICQQLEMQRIYVFSSHTPSE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be optimized for activity in riboflavin biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4, Cofactor biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The designed protein must possess zinc ion binding, GTP binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, magnesium ion binding, GTP cyclohydrolase II activity to support riboflavin biosynthetic process under certain conditions.3. A key design requirement for the protein is the optimization of the Proton acceptor; for GTP cyclohydrolase activity, Nucleophile; for GTP cyclohydrolase activity to achieve high catalytic efficiency for GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.4. Zn(2+) is required for the proper folding and stability of the protein.5. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..6. The designed protein should have a high specificity for Zn(2+), GTP, Mg(2+), D-ribulose 5-phosphate binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "RMASARLPTRFGLFYLHVYEETLNGKQHLALVKGLVDGAKNVMVRVHSSCITGDTLFSLRCDCGQQLDAALKQISQCGKGVLVYLAQEGRGIGLLNKIRSYGLQDKGLDTVEANLALGFKADLREYGIGAQILADLGLSTIKILTNNPRKIVGIEGYGLKVVERVFGGIEEKPAKKGGWDWAVPLPTRFGNFNSHLFKSELDGKEHLVLSRGLSGPGLDGPWKACDEPVMVRVHSECLTGDVFHSLRCDCGPQLDRALEILGGQERAVLVYMRQEGRGIGLENKLKAYQLQDQGLDTVEANEKLGFKADLREYGLGAQMLVYLGVRRMRLLTNNPKKIAGLKGYGLEVLEQLIG",
    "response#2": "RMASARLPTRFGLFYLHVYEETLNGKQHLALVKGLVDGAKNVMVRVHSSCITGDTLFSLRCDCGQQLDAALKQISQCGKGVLVYLAQEGRGIGLLNKIRSYGLQDKGLDTVEANLALGFKADLREYGIGAQILADLGLSTIKILTNNPRKIVGIEGYGLKVVERVIAEESGRVLRSVRESPAVPLPTRFGNFNSHLFKSELDGKEHLVLSRGLSGPGLDGPWKACDEPVMVRVHSECLTGDVFHSLRCDCGPQLDRALEILGGQERAVLVYMRQEGRGIGLENKLKAYQLQDQGLDTVEANEKLGFKADLREYGLGAQMLVYLGVRRMRLLTNNPKKIAGLKGYGLEVLEQLVAEAHLPSVYGGEWKAIAFENDVDHLDHIALVKGEFAKGESILVRVHSECLTGDVLGSKRCDCGEQLHRAMEMIEREGKGVILYMRQEGRGIGLINKLRAYELQDRGRDTVEANLELGFKADLRDYGIGAQILLTLGITKIRLLTNNPKKLIGLQGYGIDIVDREAEAHLPTKFGTFKIYAYRNKLDGSEHVALVMGDINPDEPVLVRVHSECLTGDVFGSLRCDCQSQLYKALEIIAKEGKGVLVYMRGHEGRGIGIVNKIKAYALQEQGYDTVEANHKLGFNSDLRDFGTGAQILLDLGVRKMKLITNNPRKIVALEGFDLEIVDRVERVSSVHLPTIYGDFMAHAYRFILDESPERLHIALVKGDLTAETPVLVRVHSECLTGEVFGSLKCDCGQQLHKAMELIEKEGMGVLLYMRQEGRGIGLLPKLKAYELQEKGLDTVEANLALGYPPDLRDYGVGAQILVHLGLRKIRLMTNNPKKIVGLRGYGLEVVERV",
    "response#3": "VEVDMPTKYGHFHLIPFLQKSNGLEHIALIKGKWDEDEEILVRVHSSCMTGDIFGSLRCDCGDQIHKAMELIEKEGRGVIVYLMQEGRGIGLMNKMKAYKLQEEGLDTVEANIELGFAADERDYGIGAQILRQLNVRKMRLLTNNPVKRIGLEGYNLQIVGNEAEAHLPTKFGTFKIYAYRNKLDGSEHVALVMGDINPDEPVLVRVHSECLTGDVFGSLRCDCQSQLYKALEIIAKEGKGVLVYMRGHEGRGIGIVNKIKAYALQEQGYDTVEANHKLGFNSDLRDFGTGAQILLDLGVRKMKLITNNPRKIVALEGFDLEIVDRVPVVDKRGVVEVDMPTKYGHFHLIPFLQKSNGLEHIALIKGKWDEDEEILVRVHSSCMTGDIFGSLRCDCGDQIHKAMELIEKEGRGVIVYLMQEGRGIGLMNKMKAYKLQEEGLDTVEANIELGFAADERDYGIGAQILRQLNVRKMRLLTNNPVKRIGLEGYNLQIVGNRVVEVDLPTLYGDFRLVAYQDILSEEVHLALVKGNWTEEEPVLVRVHSQCVSGDIFGSLRCDCGEQLAMAMQQVEAEGCGAVLYMKQEGRGIGLLNKLRAYKLQQEEGMDTVEANEALGFRMDHRDYGIGCQILRDLGIRKLRLLTNNPTKRVGLAGYGLEIVERLPVKDERVREAETRLPTAHGEFTAYGYRSTVDGVEHIALVAGELGDGEDVLVRVHSECLTGDIFHSLRCDCGPQLEASLQRIAEAGRGVVIYLRGHEGRGIGLLSKLRAYELQERGRDTLDANLELGLPADSRDYAAGAQMLQDLGVRSLRLMTNNPEKTSALVRYGLRVTGR"
  },
  {
    "reference": "MSVLGGKKILIGVTAGIAAYKSAWLVRGFIKAGAEVKVIMTPAAKDFVTPLTLATLSKNEVFSSFVNDDDTDNPTWNNHVELGLWADFFVIAPATANTMAKMSTGTCDNLLLATYLSAKCPVYFAPAMDLDMYRHRSTQETFEKLQSYGNIMIPAGHGELASGLEGQGRMAEPEDIVRFIEQHHLSQLPLRQKKILLTAGPTHEAIDPVRFIGNHSSGKMGYAIAERALALGAEVILISGPTHLAMHHSRLTIERVSSAQEMYEAAQLHFKEADILIAAAAVADYRPANTATQKIKKKSEALTLQLESTIDILATLGKQKQNQFLVGFALETENELEHAKAKLVRKNLDMIVLNSLKDKGAGFQTETNKITILHKDGEKINFDLKSKTEVASDILDEVIKKTDV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should interact with enzymes and substrates within the CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis, Cofactor biosynthesis pathway to enhance or regulate its activity.2. A protein with phosphopantothenate--cysteine ligase activity, FMN binding is needed to enhance pantothenate catabolic process in a specific context.3. Mg(2+) is required for the proper folding and stability of the protein.4. The protein should be designed to catalyze the following reaction H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.5. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine. The designed protein sequence is ",
    "response#1": "MNIVLGVGGGIAAYKSALLLRLFTEAGHKVTVIPTEASTKFVGVATWEALSGRPATNDVFDAVDQVNHVRIGHEADLIVVAPATADLLAKAAGGQANDLLTTTLLMARGPVLFAPAMHTEMWRHAATQANVATLRSRGIHVLDPAVGRLTGPDTGPGRLPEPEAIFAAADLAADALGVGGALAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHLVGADGDLSGLHVVVSAGGTREYLDPVRFLGNRSSGRQGYALAEAAVARGARVTVVAANVALPDPAGAEIIRVVSTEDLRQQMHLAAADADAVVMAAAPADFRPAEFVEAKIKKTADGVAPDIHLVENPDILLELVGARTSKRPVIVGFAAETGDARASVIEHARAKLARKGCDLLVVNDVSAGKVFGQDESEAVVLAADGRETAVPTGTKAEVAAAVWDLVAARL",
    "response#2": "MNIVLGVGGGIAAYKSALLLRLFTEAGHKVTVIPTEASTKFVGVATWEALSGRPATNDVFDAVDQVNHVRIGHEADLIVVAPATADLLAKAAGGQANDLLTTTLLMARGPVLFAPAMHTEMWRHAATQANVATLRSRGIHVLDPAVGRLTGPDTGPGRLPEPEAIFAAAALADAVVDALGRPLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHVLGADGAEEVVLGVSGGIASYKAAELLRLFTESGHSVRVVPTASSLQFVGAATWEALSGKPVQTGVFEHVDEVAHVRIGQHADLVLVAPATANILAKAAHGLADDLLTNTLLTAHCPVVFAPAMHTEMWNHPATQANVALLRERGVVVLEPAVGRLTGVDTGKGRLPAPAEIYAAALADALTD",
    "response#3": "MKIALGVSGGIACYKSAEIVRRLQDRAVDVVVLMTKGATQFVTPLTFRALSGNKVYLDLFEGPSSGSDFEGAFDHILLAQSIHLFLIAPATADCIARLAAGLADDFLTTFHLAVAAPVVVAPAMNTRMWEHASVQENLKTLVGRGVRIIDPEVGQMACRTYGPGRLAPVEEIVDYVMEALEAYFAQDLAGRRVLVTAGGTHEPIDPVRFLGNRSSGKMGYALAIEARNRGASVILVSGPVALPPPYGVEMQQVETAIQMRNAVHRFITDVDVLVMSAAVADFRPDEVATQKIKKHSDEVQTHDGKLSLHLVPNPDILGELADTRNGSLEDSGRRLIRVGFAAETQQVVDYAQSKLHSKGLDLLVANDVSRSDSGFGTETNKVLYPNGERIALPLMEKREIARKILELAAYQK"
  },
  {
    "reference": "MAEKPPYVKFEVPEELANKVYELVRKARETGKIRKGTNETTKAVERGQAKLVIIAENVNPPEIVMHLPALCEEKGVPYVYVPSKEELGKAAGINVSAASAAIIDAGEAKDLLEEIIRSVQELKAK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Multifunctional RNA-binding protein that recognizes the K- turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs..2. A protein that can perform rRNA binding, structural constituent of ribosome in ribosome to support translation, tRNA 5'-leader removal. The designed protein sequence is ",
    "response#1": "DKVYQAISKVRETGGKIKKGTNETTKAVERGQAKLVVIAEDVDPPEIVAHLPLLCDEKKIPYVYVPSKQKLGQAAGIEVSAASVAVIDVGGAKDE",
    "response#2": "DKVYQAISKVRETGGKIKKGTNETTKAVERGQAKLVVIAEDVDPPEIVAHLPLLCDEKKIPYVYVPSKQKLGQAAGIEVSAASVAVIDVGGAKDC",
    "response#3": "LKALEAVEIARDTGRIRKGTNETTKAVERGQAKLVIIAEDVDPEEIVAHLPPLCEEKEIPYVYVPSKKELGAAAGLEVPAASVAIIEPGKAREL"
  },
  {
    "reference": "MTDISERNKLILEAIIEDYITTAEPVGSRAVTKRHGLTLSPATVRNVMADLEEMGYLVSPHTSAGRVPTDKAYRLYVDSLLAVRRIDKVERERIRKRYAEAGRDIGAVLHETSRLLSSVSHYTGIVLAPRFSATIFRHIEFVKLGGRRILVILVADNGTVQNRLIESDEEFSSEELIRMSNYLNELLVGVPVGQVRTRILEEMRNEKVLYDKLLARALQLSEQSLNDDGAQIFIEGQTNILEQPEFADSRRMRDLFRAFEEKNQLVGLLDRCLNAQGVQIFIGAETHLSQMEGLSIITSTYLTGKNTLGVLGVIGPTRMGYAKVIPIVDYTAKLVSRLLEGE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein with DNA binding functions is required to enable negative regulation of DNA-templated transcription.2. For general function, the protein need meet that Prevents heat-shock induction of these operons.; Negative regulator of class I heat shock genes (grpE-dnaK- dnaJ and groELS operons) The designed protein sequence is ",
    "response#1": "ALSYFANELSMKSHYATLVVRPKHSEEMINNVHLIKANDSIMILVIIYNSGHVEHFHLNSALELSSDRLIAISNFISNNNIEISAKLSDKIRSFANSNEENQFINDIVKMINSHISKQSNSIFLGGKVKLIDALNESNVSSIQPILQYLESERITELLQTISTNDINVKIGKEIDESLSDISIVTSQYHFDNSLKGQIAVIGPTAMRYQNVC",
    "response#2": "ALSYFANELSMKSHYATLVVRPKHSEEMINNVHLIKANDSIMILVIIYNSGHVEHFHLNSALELSSDRLIAISNFISNNNIEISAKLSDKIRSFANSNEENQFINDIVKMINSHISKQSNSIFLGGKVKLIDALNESNVSSIQPILQYLESERITELLQTISTNDINVKIGKEIDESLSDISIVTSQYHFDNSLKGQIAVIGPTAMRYQNVL",
    "response#3": "ALSYFANELSMKSHYATLVVRPKHSEEMINNVHLIKANDSIMILVIIYNSGHVEHFHLNSALELSSDRLIAISNFISNNNIEISAKLSDKIRSFANSNEENQFINDIVKMINSHISKQSNSIFLGGKVKLIDALNESNVSSIQPILQYLESERITELLQTISTNDINVKIGKEIDESLSDISIVTSQYHFDNSLKGQIAVIGPTAMRYQNVL"
  },
  {
    "reference": "METWEVIASVKEATKGLDLSLDHPLIIKSEDVPSNILQLLQQKNRRQLKHICMKSRKEYFLLEEYGPGFWVKWPYNYFNGYSLPERRTEVVTTVERERAKRETLKTWDELKFKELLHLWSEEPKGSCKLEKDKDLKLDMNPPDMKGESKINDYYSDPKEYIESKYYDALFSIHTPLAYFVKSNLVRLKNTCRTKYGSDSYKIAYQAMLQKFLLSIVQFKDRHDNRLLLEPFSSPIADEKRKNCLTKFVIQDENKNSSTIADLCVVLKSREIKLQILLLLEIIGLNDLDWNFRDFEKKYKLKLKKRSLNLTKKGLVRRRSKKKTSEKDKGIERITTSLDYCEQLDLYLDRACILDILLSSETPNPDAIEASNGTIQEHKKNILDKSKEASLVGFINYVLIPYFNKKVPHAVEFIIQKLKGPSMRPKRALKKVNDSTNVSSPNTVETYNRLSTSQRASRSSIINSVPSSPALRRVDANLFSRKSIASPTPELLNSRTNSNLNEFLESETRSLKRPSQLGRTKSDLTMNHLQKRQFSVSDLSTTRVPNSSTITLKTPFSHSTINAYKTMNNSFRRVGKRKDINETIRLHERVDSEENVQVQATPAVKKRTVTPNKKAQLQSIIESPLNFKDDDTHEGRKNTSNITSTPTNKPPENSSKRRVRRRLFAPEST",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Required for the association of PSF1 with origins.; Required for loading and maintenance of stable association of CDC45 with chromatin during initiation and elongation of DNA replication.2. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.3. A protein that can perform chromatin binding in nucleus, chromosome, centromeric region, chromatin, DNA replication preinitiation complex to support regulation of mitotic DNA replication initiation, double-strand break repair via break-induced replication, mitotic DNA replication checkpoint signaling. The designed protein sequence is ",
    "response#1": "RSVIHSSCMDPEYYVGDEKKIDSEFSNAEKDGKDLISWLPDLSILYKLYSESGALLNLYDWYIAFSEHLQAGKENLKEDDNHPRESPQGNLQRELNELDEDKRKLEEAKLQSRFLFGLEELRFLGLIKPTARKTDHVMKTIS",
    "response#2": "RSVIHSSCMDPEYYVGDEKKIDSEFSNAEKDGKDLISWLPDLSILYKLYSESGALLNLYDWYIAFSEHLQAGKENLKEDDNHPRESPQGNLQRELNELDEDKRKLEEAKLQSRFLFGLEELRFLGLIKPTARKTDHVMKTIC",
    "response#3": "RSVIHSSCMDPEYYVGDEKKIDSEFSNAEKDGKDLISWLPDLSILYKLYSESGALLNLYDWYIAFSEHLQAGKENLKEDDNHPRESPQGNLQRELNELDEDKRKLEEAKLQSRFLFGLEELRFLGLIKPTARKTDHVMKTII"
  },
  {
    "reference": "MPTRFSTIDDTVAAIQRGEVVVVVDAEDRENEGDFVCAAEKASAEIVNFMIRHGRGQVCMPILPDVARRLDLPMMVESNSTPLGTAFTVPVDHRSARTGITAAERATAIAAILDPGSGPADFVRPGHLFPLVAKEGGVLRRAGHTEAAVDLARLAGLQPAGVLCEILDESGNRADRDELFALAGAHSLQIISIEELIRWRRTREKLVDRIAEADLPTRWGRFRVIAYGVKFEPQQPIVLVLGDVAAAREPLVRLHSSCFTGDLLDSLRCDCGDQLHLALDMIGREGAGVLVYLPQEGRGIGLVEKIRAYQLQDQGLDTVEANLALGYKADSRDYGVGIQLLKDLGLRRVRLLTNNPKKTDAFIYGGFDLEVVDQVPILPPVHEFNERYLATKREKLGHRFPE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should be able to enhance the efficiency of riboflavin biosynthetic process. The designed protein sequence is ",
    "response#1": "REASTRLPTAHGEFRAYGYRGTADGAEHVALVAGDLGDGTDVLVRVHSECLTGDVFGSLRCDCGPQLQTALREIAAAGRGVVLYLRGHEGRGIGLMPKLRAYELQERGRDTLDANLELGLPADARDYAAAAHMLTDLGVRSLRLMTNNPAKSVALAGYGLDVLSRR",
    "response#2": "EVTVDLPTKFGHFKLIAYKQINTNEEHLAIVKGKWKKNEPILTRVHSSCFTGDILGSLKCDCGDQLELALKQIEKEGKGIVLYINQEGRGIGLLNKLKSYKLQQEGLDTVEANIKLGFEMDERDYGVGAQILRDLGASKLKLLTNNPTKRTGLISYGIEIVDNIR",
    "response#3": "EVTVDLPTKFGHFKLIAYKQINTNEEHLAIVKGKWKKNEPILTRVHSSCFTGDILGSLKCDCGDQLELALKQIEKEGKGIVLYINQEGRGIGLLNKLKSYKLQQEGLDTVEANIKLGFEMDERDYGVGAQILRDLGASKLKLLTNNPTKRTGLISYGIEIVDNIR"
  },
  {
    "reference": "MPTLWLATQNQKKAVELRRILPPGNEVRSLGEVPESSSFDVVEDAPDFAGNARKKARACATWLSKHGQVLTTGDLVLADDSGLVVDALGGAPGVHSARYAGHGASDQDRITKLLGELKDQPRPWTARFACCLVAVNGKGEECFLHEAYCEGEIALSPRGSAGFGYDPVFLPQAHAGKRSFAELTPEEKDAISHRGQALAALSEHLTGR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit ribonucleoside triphosphate phosphatase activity. The designed protein sequence is ",
    "response#1": "RIFITGMPGVGKTTLALKIAEKLKELGYKVGGFITKEIRDGGKRVGFKIITLDTNEETILAYVGDGKIKVGKYAVFIENLDNVGVEAIKRALKDADIIIIDELGAMEFKSRKFSEVVDEVIKSDKPLLATLHRNWVNKFKDKGELYTLTIENREKR",
    "response#2": "KIIITGEPGVGKTTLVKKIVERLGKRAIGFWTEEVRDPETKKRTGFRIITTEGKKKIFSSKFFTSKKLVGSYGVNVQYFEELAIPILERAYREAKKDRRKVIIIDEIGKMELFSKKFRDLVRQIMHDPNVNVVATIPIRDVHPLVKEIRRLPGAVLIELTPG",
    "response#3": "KIIITGEPGVGKTTLVKKIVERLGKRAIGFWTEEVRDPETKKRTGFRIITTEGKKKIFSSKFFTSKKLVGSYGVNVQYFEELAIPILERAYREAKKDRRKVIIIDEIGKMELFSKKFRDLVRQIMHDPNVNVVATIPIRDVHPLVKEIRRLPGAVLIELTPE"
  },
  {
    "reference": "TELGTDPNAKTPSSSSSASSALSSSSSSSASSPBHRKPMDEDDNQV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Phosvitin is believed to be of importance in sequestering calcium, iron and other cations for the developing embryo.2. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "LDFTLQPGAQIHQNVPESWNAFAYILESGEGGGVFSSSNSSPIPAHSVVVFGPGNDGVSVWNKSSSKQLRFVLIAGEPIGEPVVQYGPFVMNTQAEIDMTIEDYHYGKNN",
    "response#2": "LDFTLQPGAQIHQNVPESWNAFAYILESGEGGGVFSSSNSSPIPAHSVVVFGPGNDGVSVWNKSSSKQLRFVLIAGEPIGEPVVQYGPFVMNTQAEIDMTIEDYHYGKNC",
    "response#3": "LNMKVEKEFPLLLVEKEALKKAEAQEKIDNQHGVVTRGICLSEGQIVNSVFKKPTMGPNGELVSLGTEPQKVVGGCEVTAILILYGLPRLLTGYILAHEMMHAWLRLNGYRNLKLELEEGICQVLGHMWLESQTYSSSAAASSASSSSRTPAANASKKGAQSDYEKKLVEFCKDQIETDDSPVYGVGFRKVNQMVSDSSLHKILKSIQV"
  },
  {
    "reference": "ITYTDCTESGQNLCLCEGSNVCGKGNKCILGSNGEENQCVTGEGTPKPQSHNDGDFEEIPEEYLQ",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must have high specificity towards serine-type endopeptidase inhibitor activity. The designed protein sequence is ",
    "response#1": "LSLYKQLALKDPNKNVIFSPLSISIALAFLSLGAHDHTVTEILEGLKFNLTETPETEIHQGFQHLLQTFNQPSNQLQLSVGNAMFASEELKLLDKFRKDAEAFYASEVLSTNFKDSEAAVKLINEYVKNKTHGKIEKLFNDLDVLTNLILLNYIFFKAQWKTPFNPNHTYESEFHVSQNERVIVPMMTLYLETPYFRDEELGCTLVELTYTSNDSALFILPDEGKMQDLEAKLTPETLTRWRSSLQPRLIHRLRLPRFSISSHYQLKDILSQLGIKKIFTSDAGFSGITDDHKLAVSHVIHKAVLDVGEEGTEGAAVTAVVMATSSLLHTLTVSFNRPFLLSIFCKETQSIIFWGKVTNP",
    "response#2": "DSCQLDHAQGPCLGMISRYFYNGTSMACETFQYGGCLGNGNNFASQKECLQTCR",
    "response#3": "LRLYKQLAEEIPGNILFSPVSLSSTVALLSLGAHADTQAQILQSLGFNLTETPAADIHRGFQSLLHTLDLPSPKLELKLGHSLFLDRQLKPQQRFLDSAKELYGALAFSANFTEAAATGQQINDLVRKQTYGQVVGCLPEFDRDTLMVLLNYIFFKAKWKHPFDRYQTRKQESFFVDQRLQLRIPMMRQKEMHRFLYDQEASCTVLQIEYSGTALLLLVLPDPGKMQQVEAALQPETLRRWGQRFLPSLLDLHLPRFSVSATYNLEEILPLVGLSSLFDVEADLSGIMGQLNKTVSRVSHKAVVDMNEKGTEAAAASGLLSQPPSLNMTSAPHAHFNRPFLLLLWEVTTQSLLFLGKVVNP"
  },
  {
    "reference": "PPRPLRVGLARSGVSGGARGNAGRGCGRRSASRACIRDAVSQVLKGYDWSLVPMPLKAKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGKLWRLLSETEKRPFVEEAERLRVQHKKDHPDYKYQPRRRKSVKTGQGDLDSGAELGHHLGSGAMYKADVGLGDMHHHGDNTGQTHGPPTPPTTPKTDLHHPGGGGGKQELKLEGRRLVDSSRQNIDFSNVDISELSSEVISNMDTFDVHEFDQYLPLNGHSAITAEPGQVAAGSYGSASYSHAGAATRGVSPAWAHKGASSASASPTEAGPPRPHIKTEQLSPSHYSDQLHGNSPSHADYSSYSAQACITTASPSVSSGSIPGSQCDYTDLQASGYYGPYPGYPSSLYQYPYFHSSRRPYASPLLNGLSMPAAHSPTSNWEQPMYTTLTRP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The ideal protein would be one that plays a critical role in adipose tissue development, oligodendrocyte differentiation, spermatogenesis through DNA-binding transcription factor activity, RNA polymerase II-specific, RNA polymerase II cis-regulatory region sequence-specific DNA binding within nucleus. The designed protein sequence is ",
    "response#1": "MYPGTLMSPGGDGTAGTGGTGGGGSPMPASNFAAAPAFSHYMGYPHMPSMDPHWPSLGVWGSPYSPPREDWSVYPGPSSTMGTVPVNDVTSSPAAFCSTDYSNLGPVGGGTSGSSLPGQAGGSLVPTDAGAAKASSPSRSRHSPYAWMRKTVQVTGKR",
    "response#2": "FGVQETAIISQEVHNFIMDQGWSEYQFCNQIWAGKCPKTIRMFYSNLYKKLSHRDAKSIYHHVRRAYL",
    "response#3": "FGVQETAIISQEVHNFIMDQGWSEYQFCNQIWAGKCPKTIRMFYSNLYKKLSHRDAKSIYHHVRRAYL"
  },
  {
    "reference": "MKSLLKRLIALAAAYSVAAAPSFSHHSSQDAANKRELLQDLVTWDQHSLFVRGERLMIFSGEFHPFRLPVPGLWFDVFQKIKSLGFNAVSFYTDWGLMEGNPGHVVTDGIWSLDEFFTAAREAGLYLIARPGPYINAETSAGGIPGWVLRRKGIIRSNSEDYLRATDTYMATLGKIIAKAQITNGGPVILVQPENEYTTWPNVSESEFPTTMNQEVMAYAEKQLRDAGVVVPTVVNDNKNLGYFAPGTGLGETDLYGIDAYPMRYDCGNPYVWPTYRFPRDWQHEHRNHSPTTPFAIMEFQGGSGDGWGGVTEDGCAILVNNEAVRVVYKNNYGFGVRVFNIYMTYGGTNWGNLGYYGGYTSYDYGAAITEDRQIWREKYSEEKLQANFLKVSPAYLTSTPGNGVNGSYTGNKDITVTPLFGNGTTTNLYLVRHADFTSTGSAQYNLSISTSVGNVTIPQLGGSLSLNGRDSKFHITDYDVGGFNLIYSSAEVFTWAKGDNKKRVLVLYGGAGELHEFALPKHLPRPTVVEGSYVKIAKQGSAWVVQWEVAAQRRVLRAGKLEIHLLWRNDAYQHWVLELPAKQPIANYSSPSKETVIVKGGYLLRSAWITDNDLHLTGDVNVTTPLEVISAPKRFDGIVFNGQSLKSTRSKIGNLAATVHYQPPAISLPDLKRLDWKYIDSLPEISTEYNDEGWTPLTNTYTNNTREFTGPTCLYADDYGYHGGSLIYRGHFTANGDESWVFLNTSGGVGFANSVWLNQTFLGSWTG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein with the reaction Hydrolysis of terminal non-reducing beta-D-galactose residues   in beta-D-galactosides., the Nucleophile, Proton donor must be optimized to ensure efficient catalysis.2. The designed protein should have beta-galactosidase activity within vacuole, extracellular region to regulate polysaccharide catabolic process.3. Incorporate a signal peptide in the protein design.4. The protein must exhibit the following characteristics: Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans..5. The substrate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "EIKNGLMTINGKRIVLRGVNRHEFDSVKGRAGITREDMIHDILLMKQHNINAVRTSHYPNDSVWYELCNEYGLYVIDETNLETHGTWTYLQEGEQKAVPGSKPEWKENVLDRCRSMYERDKNHPSIIIWSLGNESFGGENFQHMYTFFKEKDSTRLVHYEGIFHHRDYDASDIESTMYVKPADVERYALMNPKKPYILCEYSHAMGNSCGNLYKYWELFDQYPILQGGFIWDWKDQALQATAEDGTSYLAYGGDFGDTPNDGNFCGNGLIFADGTASPKIAEVKKCYQPVN",
    "response#2": "DLTMTEAIGNGTQYTTNTAVRAFELRNPQTNAGFYVTFHTDTTVGGNQAFKLHVNTSVGALTVPKNEGLIQLNGHQSKIIVTDFTLGKRTLLYSTAEVLTYAVFENRPTLVLWVPTGESGEFAIKGAKSGKVENGDGCSGIKFKREKDYLVVNFSQAKGLSVLRLDNGVRVVLLDKAAAYRR",
    "response#3": "DLTMTEAIGNGTQYTTNTAVRAFELRNPQTNAGFYVTFHTDTTVGGNQAFKLHVNTSVGALTVPKNEGLIQLNGHQSKIIVTDFTLGKRTLLYSTAEVLTYAVFENRPTLVLWVPTGESGEFAIKGAKSGKVENGDGCSGIKFKREKDYLVVNFSQAKGLSVLRLDNGVRVVLLDKAAAYRV"
  },
  {
    "reference": "MSNVGTTGRIPLWIIGTLAGTLVIGLLAIFFYGSYVGLGSSL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in photosystem II reaction center that is critical for photosynthesis.2. For general function, the protein need meet that PSII is a light-driven water:plastoquinone oxidoreductase that uses light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation The designed protein sequence is ",
    "response#1": "EVPDAAR",
    "response#2": "MSRWF",
    "response#3": "SEVRVYPIFTIRWLAVHALAIPSVFFLG"
  },
  {
    "reference": "MPGANQHTRFVFVTGGVASSLGKGIISASLAKLLQARGFTVTIQKLDPYINIDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNIPTSQANNVTTGRIYQAVIEKERRGDYLGETVQVIPHITDEIKYRIQLLGDCKKYDFVITEIGGTVGDIESLPYIEAIRQLKWEMGTNCLVIHLTLVPYLSAAKELKTKPTQHSVKTMLEYGVQPDILVCRTEKPLNQSIRNKIALFCNVDASSVIESIDTNSIYKVPLLMKAEKLDSVVLKKTKAPVRRPPGLKKWEGFIRKLDSPKKTIDIGLVGKYVELVDAYKSIIEALIHAGTENHCKVNLRLIHSEQLDDSNLVDKLTNLKGLIVAPGFGDRGIEGKISAITYVRKNNIPFFGICLGMQCAMIEFARNVLKIPRAHSTEMDPATPNPVIDIMEEQKKIHIKGGTMRLGAYPCELKPKSMAQKIYGQELISERHRHRYEVNNIYLERFEKAGLIASGINPETELVEIVELTDHPWFIGVQFHPEYKSTVDAPHPLFIDFVRAAKALP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should contain one or more Glutamine amidotransferase domains that are essential for its function.2. Ideal protein should have a binding affinity for key enzymes in the Pyrimidine metabolism, CTP biosynthesis via de novo pathway pathway to enhance the overall efficiency of the pathway.3. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen4. The protein should be able to bind Mg(2+), CTP ligand in a variety of conditions5. The Nucleophile; for glutamine hydrolysis of the protein must be engineered to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+). The designed protein sequence is ",
    "response#1": "MPDAYLSLLESLTHAGIANDARVNIKWVNAEELAEGDLETQLGDADGILVPGGFGIRGIEGKIKAAEYARTHNVPYLGICLGMQIAVIEYARHVAGLTGANSAEFDPYAPHKVIDLMPEQLEVEGLGGTMRLGDWPMELRAGTKIAELYGVPQGGTVRERHRHRYEVNPAYVGQLQDAGLVISGVTPGVQGRGAGLVESIEIPGHPFFVALQAHPEFKSRPMRPSPPFAGFVPDAYKSLNEALVHGGLANRVKVNIKWIDAEIFEKDPAEIAAELEPMHGILVPGGFGERGSEGKIASVSFARERKVPFFGICLGMQMACIEAARASGIQAASSTEFGETREPVVGIITEWMSEDGLQQREAGGDLGGTMRLGAYDATLLEGSKVFDVYGTCAIDERHRHRYEVDIKYKEKLENAGLTFSGMSPDGRLPEIVELNNHPWYIGVQFHPELKSKPFEPHPLFKDFISAAVNALDAYKSIYESLDHAGFSHNSRVLVKRIEAEELEEQDPEIVLSGIDGLLVPGGFGYRGIEGKVKAIQFARECEIPYFGICLGMQCAVIEFARNRLGLAEANSSEFSAETPDPVICLLEEQKTVTDKGGTMRLGTQPCQLVENSRAAACYGTSEVSERHRHRYEFNPEYEQAFEKAGMIAAGKSPSGKLVEVVEIPAHPWFVAVQFHPEFKSKPHAAHPLFRGFVEFTALT",
    "response#2": "MPDAYLSLLESLTHAGIANDARVNIKWVNAEELAEGDLETQLGDADGILVPGGFGIRGIEGKIKAAEYARTHNVPYLGICLGMQIAVIEYARHVAGLTGANSAEFDPYAPHKVIDLMPEQLEVEGLGGTMRLGDWPMELRAGTKIAELYGVPQGGTVRERHRHRYEVNPAYVGQLQDAGLVISGVTPGVQGRGAGLVESIEIPGHPFFVALQAHPEFKSRPMRPSPPFAGFVPDAYKSLNEALVHGGLANRVKVNIKWIDAEIFEKDPAEIAAELEPMHGILVPGGFGERGSEGKIASVSFARERKVPFFGICLGMQMACIEAARASGIQAASSTEFGETREPVVGIITEWMSEDGLQQREAGGDLGGTMRLGAYDATLLEGSKVFDVYGTCAIDERHRHRYEVDIKYKEKLENAGLTFSGMSPDGRLPEIVELNNHPWYIGVQFHPELKSKPFEPHPLFKDFIAAMRG",
    "response#3": "MPDAYLSLLESLTHAGIANDARVNIKWVNAEELAEGDLETQLGDADGILVPGGFGIRGIEGKIKAAEYARTHNVPYLGICLGMQIAVIEYARHVAGLTGANSAEFDPYAPHKVIDLMPEQLEVEGLGGTMRLGDWPMELRAGTKIAELYGVPQGGTVRERHRHRYEVNPAYVGQLQDAGLVISGVTPGVQGRGAGLVESIEIPGHPFFVALQAHPEFKSRPMRPSPPFAGFVPDAYKSLNEALVHGGLANRVKVNIKWIDAEIFEKDPAEIAAELEPMHGILVPGGFGERGSEGKIASVSFARERKVPFFGICLGMQMACIEAARASGIQAASSTEFGETREPVVGIITEWMSEDGLQQREAGGDLGGTMRLGAYDATLLEGSKVFDVYGTCAIDERHRHRYEVDIKYKEKLENAGLTFSGMSPDGRLPEIVELNNHPWYIGVQFHPELKSKPFEPHPLFKDFIAAMTTAVGVTIAVVGKYIDHRDAYKSIYESLDHAGFSHNSRVLVKRIEAEELEEQDPEIVLSGIDGLLVPGGFGYRGIEGKVKAIQFARECEIPYFGICLGMQCAVIEFARNRLGLAEANSSEFSAETPDPVICLLEEQKTVTDKGGTMRLGTQPCQLVENSRAAACYGTSEVSERHRHRYEFNPEYEQAFEKAGMIAAGKSPSGKLVEVVEIPAHPWFVAVQFHPEFKSKPHAAHPLFRGFLADGAAALD"
  },
  {
    "reference": "MANQREEQNTPSLPHSVFAADWIRSAEAQAAQESGTTLYGLMQRAGLAAFELARRHYPHSRHWLVLCGHGNNGGDGYVVATLARAAGLRVTLIAVPGQRPLPPEAQQAQAAWQAAGGEIHAPEDDWPEEGDLIIDGLLGTGLSAAPRAPYDRLIEQANRHPASRIALDIPSGLLAESGQTPGSVIRADHTLAFIALKPGLLTGQARDWVGQLHHSALGLEEWLQKQRVTLTRLDASSLTRWLTPRRPCSHKGEHGRLLVVGGDHGFAGAIRMASEAALRSGAGLVRVLTHKENIAPLLTARPELMVQELDDEALAAGEEWADVLVIGPGLGQSAWSKNALAALRGSDKPMLWDADALNLLAKAPDKRQNRVLTPHPGEAARLLGCSVKEVESDRLLSAKKLTECYGGVVVLKGAGTIIASESGERAIADVGNAGMASGGMGDILSGIIGGLLGQQLSPFDAACAGCVVHGHAADLIARRQGTRGLLATDLLPVIAQSVNPELK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must be optimized for ADP-dependent NAD(P)H-hydrate dehydratase activity. The designed protein sequence is ",
    "response#1": "HLPVADAAAHKYTHGHLAVFSGPLISSGAARLAAVAGLRAGAGLVTVASPPNAVLTQASHFTAIMQRALGGADALADWLGDRRLSAFVLGPGFGDADKARAFTRQLADTGRPVVLDADGITAHAACRDALGAAFGGAVRLALTPHEGEFARLFPDLAKDPALSKVDRARAAAAMLNAVVVYKGADTVIAAPDGRSAINADAPAWLATAGSGDVLAGILGGLLAQAMPVFEAACAAVAMHGLAARRAGIGMTAEDLV",
    "response#2": "PEGHKGTYGHVLVAAGTMPMSGAGLLSARAALRSGCGLVTWALPQKLLPFVIGAAPELMLAAVGSSPDGSWEAGTAAELLKLSVERDVMAVGPGLGRFTGDNDWLRRLWEETACPLVIDADALNILAEQDYSSWNNRRQPVILTPHPGEMARLAGLSTAEVQHDRIGLALAYAQEHGVILVLKGAHTVIATPEGQAYVNTTGHPGMGTGGAGDVLTGVISGLLAQGLKAVQAAAFGVYLHGLAGERAAYRRHNPASLIAGDIIEEM",
    "response#3": "PVKLPERIKWGHKGTFGKLLVVAGARYYYGAPYYSSYSFLKAGGGYSRLAAPRSVIPVLASRCSEVVYHPMEETEEGTLAYRSYESIVNIIREFGVDIVILGPGTSLNPETQDLVRALAESIEKPVIINGDGLTALSKSPDIIKRRKTPTVLTPHPVEFSRLTGINLNTLQEDPIGSVKRASCELNAYIVLKGAHSLIAYPNGKVYVNMTGNPGMAKAGMGDVLNGAIAAMYGIGLRDLGEATRIGVLVHGLAGDLVAEDIGEDGVTPDSVLEYLPRAVG"
  },
  {
    "reference": "MSQDTGLFSIKRPRESLAHFSALPQPSSALKRTSSIGAFGNPPTAQHTRMSLLNSASRPQQPNFQRSSSGGAFGADAGLSSVRRSVSSNIFHGASAGRQSYAPGSFSSNPASQNLQRRSSVFSRPSAGVGGAMGHQSFFTQVPNAAGVPRDPRPLRDRSFQARIGQELLEYLTHNNFELEMKHTLGQNTLRSPTQKDFNYIFQWLYHRIDPGYRFQKSMDAEVPPILKQLRYPYEKGITKSQIAAVGGQNWPTFLGMLHWLMQLAQMMDRFIMGEYDEACAEAGVDVSGDRIIFRFLTGAYHDWLQGGEDEDDETAGQRLVPHIEMMAQEFERGNEKYVQEVQALEAENRALRDQIEEMEKSAPDMAKLDKHFRILEDDKRKFEDYNQNVQGKIEKYENRIKFLEDELQKTEADLQAAEDERAGLQSSVDRQGITIQDIDRMNTERDRLQKILDDTMGRLEETHSRVMEKEKEASEKLEALEEVVKAYNTLGYQTSLIPSSAVNAKGQDYELSLNVNESNFSASQIGSAPSRISPEGDRLLAEPFTGYHPAHLLSLDLRGTVRSSLQTLRKDINERRKRAADDDLDRRNLLDNIKEAMDEKRSEVEALEHRRRAAEEEFERTKEITTTQKLASDAQIEKMEKELAKMRATMSESVQLMEQREMNTNIEYEQLTLRANALREELHTNVESMLNDVIRFKVHVQKGLEDYEGFVVDEVEQELGGDMVPADDIPPEEEL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.2. The protein should be designed to localize specifically to Ndc80 complex, nucleus in order to perform its role in cell division more effectively. The designed protein sequence is ",
    "response#1": "AAFPLLTAHDILECLAALDIPAQMEDLTKPTAQSTQSIYGSLLEVLMGASINSIEGPKQALLGMMEYKVLSFRSMKRTQINLSRQEMYSDTLQFMMFFKHCRRLALLCGIPDFAISDLARPDANRLRKVLSGIMNFAKFRDERMQTQARFQER",
    "response#2": "YAVPDLPVKEIMQYFSEMEINIKASDILKPTPQSTQRIYEVLLEVYCGVKTSDLLPRINSGESIEAFEESLSCILLQKRMSGFLKRIGIDNFGLRDLVPDSRRLIGILSVVVNFSMFRDNKRHVYERVCQC",
    "response#3": "YAVPDLPVKEIMQYFSEMEINIKASDILKPTPQSTQRIYEVLLEVYCGVKTSDLLPRINSGESIEAFEESLSCILLQKRMSGFLKRIGIDNFGLRDLVPDSRRLIGILSVVVNFSMFRDNKRHVYERVCQK"
  },
  {
    "reference": "MHLNRLLDVLPVKSVFGSTEHTIRSIVHDSRRVKPGDVFVAIREPTGHDGHQYIDAAIDQGAVAVISEETQRNLPITTIEVPDTHIAFSLVAAKFFDFPASQLKMIGVTGTNGKTTVTFMIDAILRAAGMRSGLIGTTGSRYMDRPVAGAKSHTTPYPMQLHEALRTFVEENAEYAVMEVSSHGLVSDRLASVKFDLAVFTNLTRDHLNDHKTIEGYREAKMKLFSSHLDDSGCAVINKDDPSYAYFLDVSAAKVLTYALSSSADISRVGPVQCYSDRTVFNVEAYIGSTFPVSIPLPGRYNASNALAAMGVALSLNIPVDAILQGLKNVRVPGRLERIDCGQPFTVLVDSAHTPDALDSVLSACREWTVGALIVVFGCGGDRDRGKRPLMAKVACNHADYVILTTDNPRMEASDIIFRDTESGLASHVAAVTIEDREEAIDHAIKYARPDDTVLIAGKGDEAFQIVNDTYIEFDDRLVARQLIETHYG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have a high specificity for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding, with minimal off-target effects.2. The designed protein should have ATP binding, magnesium ion binding to facilitate cell division, cell wall organization.3. A protein that is capable of catalyzing reactions within the peptidoglycan biosynthesis pathway with high efficiency and specificity.4. The presence of Mur ligase central domains is critical for the interaction of the designed protein with its intended targets.5. The protein have enhanced ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "MKLRELLEGITYRVVRGDSDLDVTGFAIDHRKVAPGTIFGAFQGMQVNGEDFVPAAIEAGAIAVVARPSVAVLEVPDTRRAMALLAAGYFHPVPEKLVAIGVTGTNGKTTTTHLIKSIIEECTGLPCGLVGTVAYRIGDRELPASRTTPESYDIHRLLGQMAGQGCGTAVMEVSSHALALHRVDGCRYDVAVFTNLTQDHLDFHGTMEEYAAAKRKLFEGLGPGEKAVVNLDDPWGERLAREAGARCITYGTAEGADVRAENVELRPRGTAFSLVTPRGREEVPLRMVGRFNVYNSLAAISACLLEGIPLEEAVKGLEAVPGVPGRMQRVHLGDDAPTVVVDFAHTPQAVEAAVGSLSDVGPVISVLGCGGDRDPEKREGMGAAAARGSRVTIITDDNPRTEDPAAIRAQTLAGARGVGGAEVVEVAGRRAAIEEALRRATSDSVVAILGKGHEDYQIIGREKRHFDEREVVREALCGGR",
    "response#2": "MKLSEILLPLQLKNIKSDAIYSVAINSRTVQKQTLFFALKGTHLNGADYIPQALTNGADAIISETPIPDYIYSVAINSRTVQKQTLFFALKGTHLNGADYIPQALTNGADAIISETPISQACGLLSKMFYKFPHQKLKIIGITGTNGKSSTAEFLRQLWSLQGKNAASLGTLGLITNANIPPPPSLTTPDSISLANTLALLAKNNVQHVALEASSHGLEQHRLDGVKITAAGFSNLTRDHLDYHKTIEAYRNAKTRLFADLLPHTGIAAINADMDPQTVRDLTHIAANRGIKLRSVGKRGTSLRLIHHQPTPSGQTITLALNGEELAPIDLPLPGLFQAENALLAAAIASELGIDLESIITALESAQGAPGRMQRVHLGDDAPTVVVDFAHTPQAVEAAVGSLSDVGPVISVLGCGGDRDPEKREGMGAAAARGSRVTIITDDNPRTEDPAAIRAQTLAGARGVGGAEVVEVAGRRAAIEEALRRATSDSVVAILGKGHENYQEINGVKHPFDDKEVLREYF",
    "response#3": "MMLNEILKCISVKEFIGNTSLNIYSVAINSRTVQKQTLFFALKGTHLNGADYIPQALTNGADAIISETPIPEKVIQIRVPDTRYALALISSAFFNNPSKKIKLIGVTGTNGKTTTTHLIKSIIEECTGKKVGIIGITGTNGKTSCAHLLAQTLDDPPGAAAILGTLGNGRPGHLVPSSHTTLDAVSVQAWLARFREEAVRAVAMEVSSHALDQARVAAVHFDTAIFTNLTRDHLDYHGTEAAYAAAKRRLFEMPELRMAVMWAEDPAAYTMASALSADCRQVWAGLTPRARATLPAGAARLWVRATKPRANGVDLVLDGDWGEGAVHLPLLGRFNVANALLVLAVTGTNGKTTIATLLYNLFTKAGFKVGLLSTVNVKVGDKAFDAVRTTPDSITINAYLKQMNDEGVEFCFMEVSSHGIDQHRTTALEFAGGIFTNLSHDHLDYHSSFVEYRDVKKRFFDELPASAFALVNTDDKNGQVMLQNTKAQKYTYALKSYADYKAQILENSFTGLLLKVNDQELWTRLIGSFNAYNVLAIYATANLLGMDKIAILESLSNFQGVKGRFERVQAPEGITAIVDYAHTPDALDNVLKTIDTFRAGETQVITVVGCGGNRDKTKRPVMARVAAQWSDRVFLTSDNPRDEEPEAIINDMRAGLDPIDAKKCIAITDRREAIRMAAQLAGEGDIVLVAGKGHETYQIIGKKKILFDDREVVKRFFL"
  },
  {
    "reference": "CLGVGSCNDFAGCGYAIVCFW",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Antibacterial activity against the Gram-positive bacteria B.subtilis, E.faecium and S.aureus; Inhibits chicken myosin light chain kinase with an IC(50) of 8 M; No antibacterial activity against the Gram-negative bacteria E.coli, K.pneumoniae, P.aeruginosa, P.vulgaris, S.sonnei and S.typhosa; No antifungal activity against C.albicans.2. A protein with protein kinase inhibitor activity is needed to enhance negative regulation of protein kinase activity in a specific context. The designed protein sequence is ",
    "response#1": "RLVFEGSAGLMNGFTTLTNTKKHAYGQAFNDEPFPFKNSVNGNMTSFSFTFFFAIVPEHIDKGSHGIAFVISPTRGIPGASADQYLGIFNDTNDGNSSNHIIAVELDIHKDDEFGDIDDNHVGININGMRSIVSAPAGYYDQNGQFKNLSLISGNLLRVTILYSQEEKQLNVTLSPAEEANVPKWPLLSLNKDLSPYLSKNMYIGFTASTGSVGAIHYMWMWC",
    "response#2": "RLVFEGSAGLMNGFTTLTNTKKHAYGQAFNDEPFPFKNSVNGNMTSFSFTFFFAIVPEHIDKGSHGIAFVISPTRGIPGASADQYLGIFNDTNDGNSSNHIIAVELDIHKDDEFGDIDDNHVGININGMRSIVSAPAGYYDQNGQFKNLSLISGNLLRVTILYSQEEKQLNVTLSPAEEANVPKWPLLSLNKDLSPYLSKNMYIGFTASTGSVGAIHYMWMWC",
    "response#3": "LLTLKQLGSGNSGSVSKILHIPTQKTMAKKIIHIDSKSVIQTQIIRELRILHECHSPYIIEFYGACLNNNNTIVICMEYCNCGSLDKILPLCENKQFPTFVLKKLSFAILSGLTYLYTTHKIIHRDIKPNNVLMTHKGEFKLCDFGVSRELTNSLAMADTFVGTSMYMSPERIQGLDYGVKSDVWSTGLMLIELASGVPVWSEDDNNNDDDEDDEDDAYVRQGSIAAERNGQNSPSRSRKNKQKGNGYNSYNGPEGILDLLQRIVNEDAPTLTNKINPVTKLPYDKYLCQFIDLCLIKDDSVRKTPWQLLEDKEHFS"
  },
  {
    "reference": "MLAEPVPDALEQEHPGAVKLEEDEVGEEDPRLAESRPRPEVAHQLFRCFQYQEDMGPRASLGRLRELCNHWLRPALHTKKQILELLVLEQFLSVLPPHVLSRLHGQPLRDGEEVVQLLEGVPRDISHMGPLDFSFSAGKNAPADIISEEQNSPSQVPSHSPQTELPSEEIPALHPLNELPPPQPAPIRPAEPEEWRLAPSSNWPMSPEPQEILQDPRESNPSQGPSWLEENSRDQELAAVLESLTFEDTSEKRAWPANPLGFGSRMPDNEELKVEEPKVTTWPVVIGAESQTEKPEVAGEPLTQTVGQETSSTGWGGTPADGSEVVKVRGASDAPEPQGEMQFICTYCGVNFPEMSHLQAHQLQSHPNLQPHPSSRSFRCLWCGKTFGRSSILKLHMRTHTDERPHACHLCNRRFRQSSHLTKHLLTHSSEPAFRCAECNQGFQRRSSLMQHLLAHAQGKNLTPNPEGKTKVPEMAAVLCSHCGQTFKRRSSLKRHLRNHAKDKDHLSSEDPGSLSSSQESNPYVCSDCGKAFRQSEQLMIHTRRVHTRERPFSCQVCGRCFTQNSQLISHQQIHTGEKPHACPQCSKRFVRRAGLARHLLTHGSLRPYHCAQCGKSFRQMRDLTRHVRCHTGEKPCRCNECGEGFTQNAHLARHQRIHTGEKPHACDICGHRFRNSSNLARHRRSHTGERPYSCPTCGRSFRRNAHLQRHLITHTGSKQEKEVPQECPECGKSFNRSCNLLRHLLVHTGARPYSCALCGRSFSRNSH",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Specifically binds the 5'-[GA]CGCNNGCG[CT]-3' DNA consensus sequence; Embryonic stem (ES) cell-specific transcription factor required to maintain ES cell pluripotency; Can both activate and /or repress expression of target genes, depending on the context; Regulates expression of POU5F1/OCT4, ZSCAN4 and ALYREF/THOC4.2. A protein that contributes to stem cell differentiation, regulation of transcription by RNA polymerase II, negative regulation of DNA-templated transcription by functioning in nucleus with DNA-binding transcription factor activity, RNA polymerase II-specific, sequence-specific DNA binding, RNA polymerase II cis-regulatory region sequence-specific DNA binding, DNA-binding transcription factor activity, metal ion binding.3. The presence of SCAN box domains is critical for the interaction of the designed protein with its intended targets.4. The protein should exhibit Polar residues, Basic and acidic residues compositional bias.5. The protein design should prioritize the formation of helix-turn-helix-turn-helix-helix-helix-helix elements. The designed protein sequence is ",
    "response#1": "MQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYGNGYEKFLGVCLMQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYGNGYEKV",
    "response#2": "MQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYGNGYEKFLGVCLMQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYGNGYEKCKACICDLNDDDGFTIQCDHCNRWQHAICYGIKDIGMAPDDYLCNSCITRKLLCEIFDRDTLAHHTLSGKPSPAFRDCARPSKQQLDPLKVADLVYLMTNSLDMTPREVRTAITTKPNLEIRRLEDGAEGVFAITQLVKRTQFGPFESRRVAKWEKESAFPLKVFQKDGHPVCFDTSNEDDCNWMMLVRPAAEAEHQNLTAYQHGSDVYFTTSRDIPPGTELRVWYPEGLCLMQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYSLQLPEGLCLMQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYGNGYEKFLGVPMMQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYGNGYEKFLGV",
    "response#3": "CLMQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYGNGYEKEGLCLMQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYLQLPEGLCLMQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYGNGYEKFLGVPMEGLCLMQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYVEGLCLMQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYVSVYINKEKRLQLPDFSSLPSFLPQTPSSRPMTYSYSSNLPQVQPVREVTFREYAIEPATKWHPRGNLAHCYSAEELVHRDCLQAPSAAGVPGDVLAKSSANVYHHPTPAVSSNFYSTVGRNGVLPQAFDQFFETAYGTPCLMQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYGNGYEK"
  },
  {
    "reference": "MVNIIQDFIPVGANNRPGYAMTPLYITVHNTANTAVGADAAAHARYLKNPDTTTSWHFTVDDTEIYQHLPLNENGWHAGDGNGSGNRASIGIEICENADGDFAKATANAQWLIKTLMAEHNISLANVVPHKYWSGKECPRKLLDTWDSFKAGIGGGGSQTYVVKQGDTLTSIARAFGVTVAQLQEWNNIEDPNLIRVGQVLIVSAPSAAEKPELYPLPDGIIQLTTPYTSGEHVFQVQRALAALYFYPDKGAVNNGIDGVYGPKTADAVARFQSVNGLTADGIYGPATKEKIAAQLS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. We are looking for a protein with broad substrate specificity for Hydrolyzes the link between N-acetylmuramoyl residues and L-   amino acid residues in certain cell-wall glycopeptides..2. The designed protein must have at least one LysM, N-acetylmuramoyl-L-alanine amidase domain.3. The design objective is to achieve a protein with strand-strand-helix-strand-strand-strand-helix-strand-helix-helix-strand-helix-turn-helix-helix characteristics.4. The protein must exhibit the following characteristics: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation..5. A protein that contributes to peptidoglycan turnover, sporulation resulting in formation of a cellular spore, cell wall organization, peptidoglycan catabolic process, establishment of competence for transformation by functioning in extracellular region with N-acetylmuramoyl-L-alanine amidase activity.6. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVPTIVSRKEWGARPLACRALLTLPVAYIITDQLPGMQCQQQSVCSQMLRGLQSHSVYTIGWCDVAYNFLVGDDGRVYEGVGWNIQGLHTQGYNNISLGIAFFGNKIGSSPSPAALSAAEGLISYAIQKGHLSPRYIQPLLLKEPAKYVIIIHTAGTSCTVSTDCQTVVRNIQSFHMDTRNFCDIGYHFLVGQDGGVYEGVGWHIQGSHTYGFNDIALGIAFIGYFVEKPPNAAALEAAQDLIQCAVVEGYLTPNYLLMGHSDVVNILSPGQRDRLGPRGKPSLGLHYQVRPIRYVVIHHTVTGECSGLLKCAEILQNMQAYHQNELDFNDISYNFLIGNDGIVYEGTGWGLRGAHTYGYNAIGTGIAFIGNFVDKLPSDAALQAAKDLLACGVQQGELSEDYALIAGSQVISTQSPKRDVFGPRGRPIRYVVIHHTVTGECSGLLKCAEILQNMQAYHQNELDFNDISYNFLIGNDGIVYEGTGWGLRGAHTYGYNAIGTGIAFIGNFVDKLPSDAALQAAKDLLACGVQQGELSEDYALIAGSQVISTQSPKRDVFGPRGRPIRYVVIHHTVTGECSGLLKCAEILQNMQAYHQNELDFNDISYNFLIGNDGIVYEGTGWGLRGAHTYGYNAIGTGIAFIGNFVDKLPSDAALQAAKDLLACGVQQGELSEDYALIAGSQVISTQSPKRDVFGPRGPIRYVVIHHTVTGECSGLLKCAEILQNMQAYHQNELDFNDISYNFLIGNDGIVYEGTGWGLRGAHTYGYNAIGTGIAFIGNFVDKLPSDAALQAAKDLLACGVQQGELSEDYALIAGSQVISTQSP",
    "response#2": "MLRGLQSHSVYTIGWCDVAYNFLVGDDGRVYEGVGWNIQGLHTQGYNNISLGIAFFGNKIGSSPSPAALSAAEGLISYAIQKGHLSPRGSSGRLGVRYRLGGSTKKGIDCSGFVQRTFREQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHASTSSGVIISSMNEPYWKKRYNEARYRGPANVLWKVGESAWDNNKITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQGPTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEP",
    "response#3": "MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVDGPAQTWIVADLDSGQVLAGRDQNVAHPPASTIKVLLALVALDELDLNSTVVADVADTQAECNCVGVKPGRSYTARQLLDGLLLVSGNDAANTLAHMLGGQDVTVAKMNAKAATLGATSTHATTPSGLDGPGGSGASTAHDLVVIFRAAMANPVFAQITAEPSAMFPSDNGEQLIVNQDELLQRYPGAIGGKTGYTNAARKTFVGAAARGGRRLVIAMDTGLEPLVLKPDTPTLLVVHNISLPPGEFGGPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQYQGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPDRKTDPGRPIRYVVIHHTVTGECSGLLKCAEILQNMQAYHQNELDFNDISYNFLIGNDGIVYEGTGWGLRGAHTYGYNAIGTGIAFIGNFVDKLPSDAALQAAKDLLACGVQQGELSEDYALIAGSQVISTQSPF"
  },
  {
    "reference": "MQLNPSEISELIKSRIQGLEASADVRNQGTVISVTDGIVRIHGLSDVMQGEMLEFPGNTFGLALNLERDSVGAVILGEYEHISEGDIVKTTGRILEVPVGPELVGRVLDALGNPIDGKGPVNAKLTDAIEKIAPGVIWRKSVSQPVQTGLKSIDSMVPIGRGQRELIIGDRQCGKTAVAIDTIINQKGKDLICIYVAIGQKASSIMNVVRKLEETGALEYTIVVAASASESAAMQYLAPYAGCTMGEYFRDRGQDALIIYDDLTKQAWAYRQISLLLRRPPGREAYPGDVFYLHSRLLERAARVSEEYVEKFTNGEVKGKSGSLTALPVIETQAGDVTAFVPTNVISITDGQIFLETDLFNAGIRPAINAGVSVSRVGGAAQTKVVKKLSGGIRTDLAQYRELAAFAQFASDLDEATRKQLERGRRVTELLKQPQYQPLQVWELAVSLFSANNGYLDDLDVKDVLPFEKGLREYLKTSHADLIKRIEDTKDLSKDDESALHAALKDFKKSGAY",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit proton-transporting ATPase activity, rotational mechanism. The designed protein sequence is ",
    "response#1": "RGGKIGLMGGAGVGKTVIIQEMIRRVAKEFGGVSVFAGVGERTREGNDLFLEMTEAGVIEDTALVFGQMDEPPGTRLRVALGALTMAEYFRDVQKQDVLLFIDNIFRFTQAGSEVSTLLGRMPSAVGYQPTLADEMGALQERITSTRGHSITSLQAIYVPADDLTDPAPATTFTHLDANTVLDRAISDLGIYPAVSPLDSNSRILDARYIGQEHYDTAREVQRILQRYKDLQDIIAILGIDELSEEDKILVNRARRIQRFLSQPH",
    "response#2": "QGGKAAMFGGAGVGKTVLVMELIHAVVERYEGISVFAGVGERSREGHEMLLDMRTSGVLPRTVLVYGQMNEPPGARWRVPFTALTIAEYFRDERRQNVLLLMDNVFRFVQAGAEVSGLLGRMPSRVGYQPTLATEVASLQERIVSVGGVSVTAIEAVYVPADDFTDPAVTAIAAHLDSMVVLSS",
    "response#3": "GQRVFDTFFPLGMGGTAAIPGGFGAGKTMTQHQLAKWSSADIIVYVGCGERGNEMTDVLEEFPKLEDPSTGKSMMERTVLIANTSNMPVAAREASIYTGITIAEFYRDMGYNVALMADSTSRWAEALREISGRLEEMPAEEGFPAYLPSRLAEFYERAGYVKTLGQDREGSISLIGAVSPPGGDFSEPVTQNTKRYVRCFWALDKSLASARHFPAVNWLESYDDWYLENISEDIIELRNKMLKILFEENKLQEIVKLVGEDVLPDDQRLILEVARILKVGFLQQNAYHDEDTYVPKEKQYKMLKAIELFYDNAYKCVKMGIPISKK"
  },
  {
    "reference": "MDKVIIMAAGEGTRMKSNISKVLHKLCNKTLLSYVIESSQEAGIDDIIVIVGNNEDAVKEIFGDSVKYVRQYIGENYPYGTGYAVKLAEQYINEGDNILVLSGDAPLIKSETIRNLIDFHNESKNDATVLTAIFENPFGYGRIVTDKNGKLLKIVEHKDANDNEILINEINSGIYLFNGDKLRYALNKLDTNNSQKELYLTDVFEILKKEKLNISTFKVKDTEEIKGINTKIQLSEVEKIMRRRINEEYMLNGVILENPETITIQKGIRIGRDTVIESNVKILGDTIIGENCLIGMNSRIEDSKIADDVKILSSVIEKAIVEKGADVGPFSRLRPKAHLKERVHIGNFVEVKNAVVGEGTKAGHLTYIGDADLGKNINVSCGVIFANYDGKNKFRSTVGDSAFIGSNVNIVSPVNIEEKAFLAAGSTITREVKAGQLSIERAEQKNIDGYYKRKFEK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Proton acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.2. The designed protein should have a binding site that is specific to the target UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA, Mg(2+) ligand.3. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.4. The designed protein should contain one or more Nucleotidyl transferase domains that are essential for its function.5. A protein that contributes to cell wall organization, UDP-N-acetylglucosamine biosynthetic process, regulation of cell shape by functioning in cytoplasm with glucosamine-1-phosphate N-acetyltransferase activity, magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity. The designed protein sequence is ",
    "response#1": "MTLILAAGEGTRMRSSTPKVLHPIAGRPLVEHAVRAAAGLNPEHLVVVVGHGRELVGEHLAQLGTALGRPVGTAVQDQQKGTGHAVSCALATLPADVTGTVIVSYGDVPLLDTTTLAALLDEHTEAKNAVTVLTTVLEDEPTGYGRVIRDADDVVTAIVEHKDASESQRAIREVNAGLYRFDADFLRGALKRVTTDNAQGEMYLTDVLGIARADGQSVGALVAADHREIAGVNTRVELAAARAIMNHRTLERHMLAGVTVIDPASTWIDVDVMIGRDTVVRPGTQLLGGTRIGSRCDIGPDTTLTDVTVGDAAEVVRTHATSAEIGDEATVGPFTYLRPGSKLGSAGKLGAFVETKNAAIGAGSKVPHLTYVGDADIGEHSNIGASSVFVNYDGETKSRTTIGSHVRTGSDTMFVAPVTVGDGAYTGAGTVIRDDVPPGALAVSAGPQRNIEGWVERRRAGTAAADAAVSARSQETTGSTPETSE",
    "response#2": "LILAAGAGTRMKSDTPKVLHTLAGRSMLAHSLHAVTKVAPRHLVVVAGRDRERVAPAALDIGRDLGRDVQIAVQEEQLGTGHAVVCGLSALPEDFTGVVVVTAGDVPLLDAATLAELVATHSAEAALVTVLTTTLPDPTGYGRVLRTQDGDVIGIVEQADATPSQQAITEVNAGVYAFDIAALRSALSRLSADNAQHELYLTDVIAIVRADGHLVRAHHVDDYEETLGVNDRVALAEARTELAQAEALFQARARAEALENGVTLQAPETVFFAHDTYVGRDTVVEPNVVFGPGVTVESGAEIRAFSHLEGCHVSQGAQVGPYARLRPGAELAEKVRIGNFVEVKNAILDEGAKVNHLSYIGDATVGAATNIGAGTITCNYDGVMKHRTEIGNHAFIGSNTMLVAPVKVGNRAMTGSGSVITKDVEADALAVSRARGVPAHPIRRGKTNRAELARAEALFQARMRDEALENGVTMVDPGTVWFALDTYLGRDVVLGPHVVFGPGVTVESGAQIEAFCHLEGCHISRGATVGPFARLRPGAELAEEVHIGNFVEVKNAILGEGVKVGHLTYLGDAEVGEGTNIGAGTITCNYDGVFKHRTTIGANAFIGSDTMLVAPVTVGAGAMTGSGSTITEDVPPGAVALGRAKVILAAGMGSRMNSDLPKVLHKIAGAPLLVHAITSGAAVNPSRLVVVAGHGADQVRSAVEDWAPEAEVVIQAEQLGTGHAVLQAREALEGFEGDVIVLYGDTPFIQPDTIARMVEARQQHDVVVLGFERKVTIGEGWREQNRAG",
    "response#3": "MSAVILAAGEGKRLKLDAPKPLAPCLDKKLVDFPIRELQKFFSRNNLTGTQTAVIGHQKELVQSYISSRYPEVKFAVQERQLGTADALRAYFNSSSNTHQFDYTLVICADTPVVSEKELTQMLELLKQEGCDGVAATFTESNPKGYGRIIRGTKGFHIVEEKDATDEQRKITEVNSGLYLLKTSYVLEHLKNVDSNNKSGEFYLTDVFKTVDEALGVNDRVALAQAEAIMRERINKKHMRNGVTFIDPEHTYIGSDVSIGTDTIVYPGVIIEGKTSIGNNCVIHANSRIKDCIIDNEVEIDNSTVLASKVMNNAKIGPYAYVRPNCIIGERVKIGDFVEVKNSTIGNDTKASHLTYIGDAKVGDRVNLGCGVVFVNYDGQYKHETIVEDDCFVGCNVNLIAPANVKKGSYVAAGSTITESVEENSLAIARCRQINKEGWADRKRK"
  },
  {
    "reference": "MAAPVRGMAVSSSIGNTTSFQKKTRSKLVFIPGTRPSVQNGQLLVSSGVSSLDYVIGGGLAVGTLLLVEEDRYDSYSRMLLKYFLAEGIVCGHELFLASARDHPDQIMQELPSPILDDVASMKMSEGQSQPNDPDNPDTMKIAWRYQNQPRVQTALASSSRFGHYYDASKTMDPELLQAAKYHSFYQLQETPVTTGLSSLPSPYLALLKSIQTLIQKEGFDGSTPQLRGRNVLRVGLHSLGSVLWGDDVCCKDNSAHCHALSTFLYALRGLLRTSLSVAMMTVPSHLIQSRAVMGRIIRLSDTAIALESFRGSEKETNPLYKDYHGLLYVRQIPRLNCLTSEVPDTKDLAFKLKRKQFTIERLHLPPDLSETVSRVSKADLAAGCASTATGNKHLHF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm, elongator holoenzyme complex, transcription elongation factor complex that is critical for regulation of transcription by RNA polymerase II. The designed protein sequence is ",
    "response#1": "DPTVANRLDSQYIQTASDLRNYRDGTEIIAYASGKTGSVLNIAVLTRQNTLHLNRHNNVTSIELHSPIKSIKIPGASESIGRRSNLVGIITENSFQIFRIESVHSRSCDVMVSSSEPLYFVEIDDLQVVDFAFNPWDLQQFAIIDIKGNWSIGRIPKNFNNNNKRKLQLIDNLHGTIFDPEELSSWKRIEWFSHFQKILVFDRSKMIEIDFMNNWQTEVVQAKAWSNIRDYKRIDDKNGILLTSREIIIVGASESNDPVRRISWKHDLDPDDTTLRITVQKVKKPDHILLVAFVYSMRHKRIYMHVFSHRKANLFQSLGCSTVLEIC",
    "response#2": "DPTVANRLDSQYIQTASDLRNYRDGTEIIAYASGKTGSVLNIAVLTRQNTLHLNRHNNVTSIELHSPIKSIKIPGASESIGRRSNLVGIITENSFQIFRIESVHSRSCDVMVSSSEPLYFVEIDDLQVVDFAFNPWDLQQFAIIDIKGNWSIGRIPKNFNNNNKRKLQLIDNLHGTIFDPEELSSWKRIEWFSHFQKILVFDRSKMIEIDFMNNWQTEVVQAKAWSNIRDYKRIDDKNGILLTSREIIIVGASESNDPVRRISWKHDLDPDDTTLRITVQKVKKPDHILLVAFVYSMRHKRIYMHVFSHRKANLFQSLGCSTVLEIC",
    "response#3": "DPTVANRLDSQYIQTASDLRNYRDGTEIIAYASGKTGSVLNIAVLTRQNTLHLNRHNNVTSIELHSPIKSIKIPGASESIGRRSNLVGIITENSFQIFRIESVHSRSCDVMVSSSEPLYFVEIDDLQVVDFAFNPWDLQQFAIIDIKGNWSIGRIPKNFNNNNKRKLQLIDNLHGTIFDPEELSSWKRIEWFSHFQKILVFDRSKMIEIDFMNNWQTEVVQAKAWSNIRDYKRIDDKNGILLTSREIIIVGASESNDPVRRISWKHDLDPDDTTLRITVQKVKKPDHILLVAFVYSMRHKRIYMHVFSHRKANLFQSLGCSTVLEIC"
  },
  {
    "reference": "MVTLISYISLLAGFVIIASVFYLALVKIKLI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity. The designed protein sequence is ",
    "response#1": "FPIYAQQAYESPREATGRIVCANCHLAQKPVEIEAPQAVLPNTVFETVVKIPYDNNAKQILGNGSKGGLNVGAVVILPEGFKLAPANRLSPELKEKTKNLYIQPYSTKQSNILVIGPIPGDKNREIIFPILSPDPAKDKQAHFFKYPIYVGGNRG",
    "response#2": "FPIYAQQAYESPREATGRIVCANCHLAQKPVEIEAPQAVLPNTVFETVVKIPYDNNAKQILGNGSKGGLNVGAVVILPEGFKLAPANRLSPELKEKTKNLYIQPYSTKQSNILVIGPIPGDKNREIIFPILSPDPAKDKQAHFFKYPIYVGGNRG",
    "response#3": "FPIYAQQAYESPREATGRIVCANCHLAQKPVEIEAPQAVLPNTVFETVVKIPYDNNAKQILGNGSKGGLNVGAVVILPEGFKLAPANRLSPELKEKTKNLYIQPYSTKQSNILVIGPIPGDKNREIIFPILSPDPAKDKQAHFFKYPIYVGGNRG"
  },
  {
    "reference": "MHQRGVGDFDAMSDLARDFRAQLAGQAEIATPPVLTEQRSTDGTRKWLFDVGQGNAIETVFIPEDDRGTLCISSQAGCTVACRFCSTGHQGFNRNLTTAEIIGQLWHARRTLSDDSGSARLAGAGQADAPAKLISNVVMMGMGEPLLNYEPVLAALRLMLDDNAYGLSRRRVTVSTSGVVPMMDKLGRDCPVALAVSLHAPNDALRDDLVPLNRKYPLAELLAACERYLEHAPRDFITFEYCMLDGVNDSDAHARELVRIARQVRCKLNLIPFNPFPQSGLKRSPAARVKQFAQRLMDADVLTTVRKTRGDDIDAACGQLAGDVKDRTRITERLSTRKNAIPIVELRA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine are properly positioned for optimal catalysis.2. A protein that can perform rRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding, metal ion binding in cytoplasm to support rRNA base methylation.3. The protein contains novel Radical SAM core domains that confer a unique function or activity.4. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs5. The protein design should incorporate a binding pocket that specifically recognizes [4Fe-4S] cluster to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "QAGRGTLCVSSQAGCALDCSFCSTGKQGFNSDLTSAEIIGQVWIANKSFGTVPGKIDRAITNVVMMGMGEPLLNFDNVVTAMSIMMEDFGYGISKRKVTLSTSGVVPMIDKLGETTDVSLALSLHAPNDELRNQLVPINKKYPLKMLLDACRRYITRLGKERVLTIEYTLLSNVNDQPEHAEQMIALLKDFPCKINLIPFNPFPHSGYERPSNNSIRRFQDMLHKGGFNVTVRTTRGDDIDAACGQLVGQVQDRTRYFARRAHP",
    "response#2": "EGLGEPSYRGGQILEWLWQKRATSIEGMSNLPVRLREHLEESFTLRSLSPAKSQGSRDSTRKFLFRLNDGRYIECVLIPASPALYGERSDRRTLCVSSQVGCAFGCKFCASGLDGFTRNLTADEIAGQVLLAEELSGEKVNNIVFMGMGEPLANLPQLLKALELITSPWGLGIGARHLTVSTSGLVPQIRKLADHPQQIRLAISLHGASNDVRDRIMPVNRKWPVEDLFKALSYWTKRKNQKITLEYILIDGINADPAQARLLAQHAESLRAKVNLIPYNTVDGLKWKRPPEEECHAFRDILAARGIPATLRLEKGHDIDAACGQLRLKEERAEKG",
    "response#3": "ISTQVGCAMGCVFCASGLLGVRRNLTAAEIYEQVLVLHRLLQPDEKITNLVVMGIGEPLANFRSLTAALELLTADDGFGLGARRITVSTVGLPEKIREFARLGSQYNLAISLHAPNDELRTQIVPVNRSIGIAEILDAADDFHQITGRRVSWEYVLLAGVNDQDGLGRIVRDDKGQVLRIVEEKDATPEQLAITEINSGVYCAALDFLRQAITQLESNNAQGEFYLTDVIAMAVADNLPVSAVLPDDVTEVLGINDRVQLAEAGRLLQVRTARALMLA"
  },
  {
    "reference": "MKVKGIRKNCQHLWRWGTMLLGMLMICSATGKLWVTVYYGVPVWKEANTTLFCASDAKVHDREVHNVWATHACVPTDPDPQEMVLENVTENFNMWKNNMVEQMQEDIISLWDQSLKPCVKLTPLCIILNCIELNATAILNTTNTTNRNSGREMIEEMRNCSFNITTSKKFKMQKEYALFDSLDIVPIDNDNTSYRLTSCNTSVITQACPKVTFEPIPIHYCAPAGYAILKCKDKKFSGKGQCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVLRSDNFSDNAKNIIVHLNESVEINCTRPHNNTRKGIHMALGRAFFVTEDITGDIRQAHCNISRVKWERALEQIVEKLREQYKNTTINFNQSSGGDPEIVMHSFNCGGEFFYCNTTQLFNSTWNKNSTRNNTSGTITLPCRIKQIINMWQEVGKAMYAPPIKGQIKCSSNITGLILTRDGGSNKNETENKTETFRPGGGDMRDNWRSELYKYKVVRIEPLGIAPTQAKRRVVQREKRAVTLGAVFLGFLGAAGSTMGAASIALTGQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLQDQQLLGIWGCSGKLICTTTVPWNASWSNKSMNQIWDNMTWMQWDKEISNYTGLIYTLIEESQIQQEKNEQKLLELAKWDSLWNWFDISKWLWYIKIFIMIIGGLVGLRIIFAVLSIVNRVRQGYSPLSFQTRLPTPRGPDRPEGTEEEGGERDRGRSGRLVNGFLALIWDDLSSLCRFSYH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that can perform structural molecule activity in host cell plasma membrane to support positive regulation of receptor clustering, positive regulation of establishment of T cell polarity.2. For general function, the protein need meet that Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes3. The designed protein have Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MRVKGIRKNYQHLWRWGIWRWGIMLLGMLMICSATEKLWVTVYYGVPVWRDAETTLFCASDAKAHETEVHNVWATHACVPTDPNPQEIHMENVTENFNMWKNNMAEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTNANLTNATVTNDNNTTTALKTIGNITDEVRNCSFNMTTVVRDRTQQVHALFYRLDIVPIDNDNNSNSYRLINCNTTVIKQACPKISFDPIPIHYCTPAGYAMLKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEVIIRSENITDNAKTIIGHLNKSVEINCTRPSNKIRTSMRIGPGQVFYKTGDIIGNIRKAYCEINGTQWYESLKQVQKKLQQHFFNKTITFQPQSPSGGDLEITMHHFNCRGEFFYCNTSKLFNNTCINETQEGCNGTIILPCKIKQIINMWQGVGQAMYAPPISGTINCVSNITGILLIRDGGVNNTNETFRPGGGNMKDNWRNELYKYKVVQIEPLGIAPTRAKRRVVEREKRAVGIGALFLGFLGAAGSTMGAASVTLTVQARQVLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTNVPWNSNWSSKSLDDIWDNMTWMQWDKEINNYTQLIYSLLEESQNQQEKNEQDLLALDKWANLWNWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSIVKRVRQGYSPLSFQTLFPVPRGPDRP",
    "response#2": "MRVMGILRNCQQWWIWGILGFWMLMICNVVGNLWVTVYYGVPVWKDADTTLFCASDAKAYETEAHNVWATHACVPTDPNPQEIPLENVTEQFNMWKNNMVEQMHTDIINLWDQSLKPCVKLTPLCVTLNCTKPNNTTLWVTVYYGVPVWKDATTTLFCASDAKAYVTEAHNVWATHACVPTNPNPQEVVLNVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVRLTPSCVVLNCTDYTPNATKTNNTSRESNSTVEDKGEIKNCSFNITTSIKTKVKDYALFNKVDVVPIENDTTSYRLNYCNTSVITQTCPKVSFEPIPIHYCTPAGFAILKCNDKKFNGTGPCKNVSSVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSSNFTNNARVIIVQLNKSVEINCTRPNNNTIKSIPLGQGRAWYTTDQIIGDIRQAHCNLSSKDWNNTLRQIAEKLREQFGGNKTIVFNQSSGGDPEITVHSFNCGGEFFYCNTSKLFNSTWHKNSTWNNTKDENITLPCRIRQIVNMWQEVGKAIYAPPIKGQIRCSSNITGLLLTRDGGENQTDREIFRPGGGDMKDNWRSELYKYKVVKIEPLGVAPTRAKRRVVQREKRAVGIGAVFLGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLALERYLRDQQLLGIWGCSGKLICTTTVPWNSSWSNKTYNSIWDNMTWLQWDREISNYTELIYSLIEESQNQQEKNEKELLALDKWASLWNWFDITSWLWYIRIFIMIIGGLIGLRIVFAVLNIINRVRQGYSPLSFQTLTH",
    "response#3": "LWVTVYYGVPVWKDAETTLFCASDAKAHETEAHNVWATHACVPTDPNPQEINLGNVTEEFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLECSNVNGTNSTNXTSDSKLEEEIGEIKNCSFNMTTELRDKQRKVYSLFYKLDVVQMGDNNSSKYRLINCNTSAITQACPKVTFEPIPIHYCAPAGFAILKCNDEEFNGTGPCMNVSSVQCTHGIKPVVSTQLLLNGSLAEGTIKIRSENITNNAKNIIVQFTESVKIDCIRPNNNTRTSVRIGPGQTFYATGGIIGNIRKAHCVVNGTKWNRTLSKVVNQLRTYFGNKTIIFDKSAGGDLEITTHSFNCGGEFFYCNTSSLFKGTWNNGTWNDSMSEVNSTESNDNITLPCRIKQIINMWQKVGQAMYAPPIQGVIRCESNITGLILTRDGGNSSGQNETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKARRRVVQREKRLLLGMLFLGSLSAAGSTMGAASITLTVQARQLLSGIVQQQNNLLRAIDAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLNKIWDNMTWMEWEKEIDNYTSLIYTLIEESQNQQEKNEQELLELDKWASLWNWFDITNWLWYIKLFIMIVGGLVGVRVVFTILSIVSRVRQGYSPISFQTRLPTQRGPDRPEGIEEEGGEQGRDRS"
  },
  {
    "reference": "MGKSSPVCLYLILQGLLLFDTVNAKNLNELKMECPHTIGLGQGLVVGSVELPPVPIQQIESLKLESSCNFDLHTSTAGQQSFTKWTWETKGDLAENTQASSTSFQTKSSEVNLRGLCLIPTLVVETAARMRKTIACYDLSCNQTVCQPTVYLMGPIQTCLTTKSCLLGLGDQRIQVNYERTYCVSGQLVEGVCFNPIHTMALSQPSHTYDIVTIMVRCFLVIKKVTSGDSMKIEKNFETLVQKTGCTANGFQGYYICLIGSSSEPLYVPTLDDYRSAEVLSRMAFAPHGEDHDIEKNAVSALRIAGKVTGKAPSTESSDTVQGIAFSGSPLYTSTGVLTAKDDPVYVWAPGIIMEGNHSVCEKKTLPLTWTGFIPLPGEIEKTTQCTVFCTLAGPGADCEAYSETGIFNISSPTCLINRVQRFRGAEQQIKFVCQRVDMDITVYCNGVKKVILTKTLVIGQCIYTFTSIFSMIPGIAHSLAVELCVPGLHGWATVLLLLTFCFGWVLIPTITMILLKILIAFAYLCSKYNTDSKFRILVEKVKKEYQKTMGSMVCEVCQYECETAKELESHRKSCSIGSCPYCLNPSEATPSALQAHFKVCKLTSRFQENLKKSLTMYEPMQGCYRTLSLFRYRSRFFVGLVWCMLLVLELIVWAASAETQNLNDGWTDTAHGSGIIPMKADLELDFSLPSSASYTYRRQLQNPANEQEKIPFHLQISKQVIHAEIQHLGHWMDATFNLKTAFHCYGSCEKYAYPWQTAGCFVEKDYE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Attaches the virion to host cell receptors including integrin ITGAV/ITGB3; Class II fusion protein that promotes fusion of viral membrane with host endosomal membrane after endocytosis of the virion.; This attachment induces virion internalization predominantly through clathrin-dependent endocytosis.2. Incorporating a YxxL motif is critical for the successful design of the protein.3. The designed protein should contain Lumenal topological domains.4. The ideal protein would be one that plays a critical role in viral entry into host cell through metal ion binding within host cell endoplasmic reticulum membrane, host cell surface, host cell mitochondrion, host cell Golgi membrane.5. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "TDGSTECNSGTQLIEGKSIEVHKQDIGGPGKKLTICNGTSVLDVPLDEGHGCYTINVITSKRACRPKNSKLQCSIDKELKPCDSGKCLSISQKGAGHIKVSRGKTILITECKEHCQIPVPTGKGDIMVDCSGGRQHYLEVNIVDIHCPNTKFLGGIMLYFCRMSSRPTVALLLGIWIGCGYILTCIFSFLLYHLILFFANCIKQCRKKGERLGEICVKCEQQTVNLMDQELHDLNCNFNLCPYCCNRMSDEGMSRHVGKCPKRLERLNEIELYLTTSECLCLSVCYQLLISVGIFLKRTTWLVVLLVLLGLAISPVQGAPLAKWREAEEMTRDETRDGSFRFTSRALGASFVSDVTQLDLQRVHLGDCVLREASEAIDAIYRRRYNSTHVLAGDRPEVYLARGGFVVAFRPLISNELAQLAKWREAEEMTRDETRDGSFRFTSRALGASFVSDVTQLDLQRVHLGDCVLREASEAIDAIYRRRYNSTHVLAGDRPEVYLARGGFVVAFRPLISNELAQLAKWREAEEMTRDETRDGSFRFTSRALGASFVSDVTQLDLQRVHLGDCVLREASEAIDAIYRRRYNSTHVLAGDRPEVYLARGGFVVAFRPLISNELAQLAKWREAEEMTRDETRDGSFRFTSRALGASFVSDVTQLDLQRVHLGDCVLREASEAIDAIYRRRYNSTHVLAGDRPEVYLARGGFVVAFRPLISNELAQWAHWKSFDNAIKTEHGKSLHFVANDITASFYTPNTQTREVLGKHVCLNNTIESELKSRLAKVNDTHSPNGTAQYYLTNGGLLLVWQPLVQQKLL",
    "response#2": "VECPHTIGLGQGIVIGSAELPPVPLAKVESLKLESSCNFDLHTSTAAQQAFTKWSWEKKADTAENAKAASTTFQSSSKEVQLRGLCVIPTLVLETASRTRKTVTCFDLSCNQTVCQPTVYLMAPIQTCVTTKSCLLGLGDQRIQVVYEKTYCVSGQLIEGNCFNPLHTIAISQPTHTYDIMTLAVHCFFISKKGGTDDTLKIEKQFETLVEKTGCTENALKGYYACILGTSSEVVYVPAMDDYRSSEILSRMTTAPHGEDHDIDPNAISSLRIVGQLTGKAPSTESSDTVQGIAFAGTPLYTSTSILVRKEDPIYLWSPGIIPEGNHSQCDKKTLPLTWTGFITLPGL",
    "response#3": "MKEEIKNCSFNITTELRDKKKKESALFYRIDIVPLNEKSNNSNGSSEYRLINCNTSTITQACPKVSFDPIPIHYCAPAGYAILKCNDKTFNGIGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENLTNNAKTIIVHLNKSIAIMCTRPGNNTRKSVRIGPGQTFYATGGVIGDIRQAFCIINGSNWNKTLQEVEEKLKRHFPNATEIKFEPSSGGDLEVTTHSFNCRGEFFYCDTTILFNRTMITDNINTLPCRIKQIINMWQGVGRAMYASPIAGNITCISNITGLLLTRDGGKTNSTTETFRPGGGNMKDNWRSELYKYKVVEIKPLGIAPTGAKRRVVEREKR"
  },
  {
    "reference": "MAASQTIADSKRAFHQAFPHVIAPLYRRLADELLVELHLLSHQSRFEANELFSVGLCTVFDTFIKGYRPEAQTDALFRALCSSNGFDAAKLRKTYASLVEQAKGKDPESLKDWLSSHALKEGSHYSRLMAVGLMSLLKAAAADATDSDTEAIVKQSKELAEGLGLPTDRVEKDLTLFGSNSERMDQAVELVEETIAAEKRKKERRLEEQAQRTSS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. Ensure the presence of a signal peptide within the protein.2. The protein design should aim to mimic or enhance the function of natural proteins involved in photosystem II assembly, potentially with improved properties such as stability, specificity or selectivity.3. For general function, the protein need meet that May be involved in photosynthetic membrane biogenesis. The designed protein sequence is ",
    "response#1": "MF",
    "response#2": "PQALNNIAVIYHYQAVKASEINDLETAQALFHEA",
    "response#3": "PPQALNNIAVIYHYQAVKASEINDLETAQALFHEC"
  },
  {
    "reference": "MSNHSDQIVKKYILNQSFVESSFFTKLSELKLEKYKLDSSYIAIHGFQTHPTKLNKFNDTPVLNLDQSSFDDTLNDSRINIPGELFNVNTIEEFKSLDKLKLLNTWGQNVYSEVTNATSFDYKLFNKFYILTYSDLKKYKFYYWVAYPTLSNTWTVESESQETDTTITQLVETELDNEYGQFFQYYGGKLHKSVQADKEHTFVFIDTCLSKDRKPSSQLKNYLYFIAYKGIKEIDLVTYRNNNLSFTQHLKLDAFTDSPKISGWERTNQGKLGPKLADLGSLINPLQLAEQAVELNLKLMKWRIAPDIDLEIIKKQKVLLLGAGTLGSYVARALLGWGVRSITFVDSGRISFSNPVRQPLFNFEDCFSDSGQGEYKALRAAENLKRVFPGVDAKGICLAVPMVGHPVTDEHKERENYETLVKLFEEHDVIFLLMDSRESRWLPTLIGAANEKIVINAALGFDSYLVMRHGVTNQKDRLGCYYCNDVVAPNDSLSDRTLDQMCTVTRPGGALMASSLAVELLVAILQHPERNLAPHDAETKFGNIPHQIRGFLHNFQQTKLFAPSYVHCSACSPRVITEFKQEGWEFVRKCLDDSQYLEDISGLTKVQQEAELAAKQLLEDLSLDDDVSNDIDEDSEWLS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production; Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes; Plays a role in the regulation of filamentous growth and chronological longevity .; Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation.2. The designed protein must have a Glycyl thioester intermediate active site that is highly conserved among related enzymes.3. The designed protein should have identical protein binding within extrinsic component of phagophore assembly site membrane, cytoplasm, phagophore assembly site to regulate C-terminal protein lipidation, late nucleophagy, autophagosome assembly. The designed protein sequence is ",
    "response#1": "TLTNQNDLQAWRNLYMARRSWALGLVERCGAMDKSIHEHNERTDIIHRAAGVALENLKTHVGNLENRFQEAQTWANDLLKEQRAALDGWQRALTTLESIPAPKVFPFLGRPSTPKEHRDRPTGTLRDFVDANEVQKAGAEAAAESSRFARQIDDVAEAVRGITADTQRLIDDQLPSGADAADGLQEMITFAKKISSDYEHVIALPNNQKTLANISRLALTHTQDLLPSMLDISAEIHAGLEEAVRRHNTAMKVALDHMRTISAIELRLADVQSQIINLNVQSDAFDVVFSVYHMPMVYGSILVESVRRREFNEKMKADSLTLAEEMAVFRDEEQRRRKKWLKSMGDFL",
    "response#2": "IQNFHTKAAIIVLGARVGLPVVFTKEGTKKVNKWFQIETDDTDAYRDELHTWRMCGGFQNRPPPLIIETYLDTSHLTSSQRLVIVDDLGKRWDALDALSNSCGSNDGRSEVILERWKIELRDIPGEQPYDFGSTLPTIYKKCIVFFRSLYSTAKLVPAWKFKKSLGKNGSPTNSLIINCRITTGDTQPRRIDGLKQPILDHSGPVTSTYVLGATDTPAGQIFAEVTYRNDCNWRISPQLNLEKVAQQKVLLLGSGTLGSYVARALMGWGVRHITFVDNGRVSYSNPVRQPLFGFNDCFSDEGLGRWKAPRAAEALKEIFPGVNSSAYNLEVPMIGHPVENEQSAKANYETLERLVDENDAIFLLMDSRESRWLPTLLGMAKNKLVINAALGFDSFLVMRHGTTTNNLGCYYCNDVVAPSDSLSDRTLDQMCTVTRPGGALMASALAVELLVSVLQHPEGAEADACGEALKYVPTRSFVESSFFKALSDLKLNQLKLNTDTVSISGFMNHPKNLTKFQDYPILNLDFSSFELASSASSAASDENVEYKGTLLNVNTIEEFKAINKSDLLKQWGSLIYKSITEGDTTYRSVNQFFVLTFSDLKKYKFYYWFAYPSLQSQWTVKECKEAPSFEDETRKYLDTQEELEFFFAREQEEFCGLSKFLNGSKKVNEHSTFVFVDSCRHPGLYPGWQLKNFLYILARQGMREIRLFVYRYDGTSIMMKLQLDSIPNDIKFMGWERTKDDKLGPKLADLGS",
    "response#3": "LKFAPPFQSFVDASFFQVFSRLKLDVLRLDSHELPLHAKVDLAGLARGSSISHVFLDSQSFDEATASLPGISLRGSFFNFNTLEEFKRLDKGRFLSEQAQLLWEAGVNGYLDEAAGFFVICFADLKKYRFYYWFATPCFQPETLELKVVKREALTEIDKFSNFIEQNKILCGVLNEETGEVIRASRHELERYSTLVVRDTSNIEHCPTSLVKNFVAVWRHHNPNRSECRVLLLRETCSFSLELSVTGDAMSTSQLKASGWERNVRGLLTPKISELGALPATVPLAPPEPSLSSINHVWRANEIVTAARAALEECVVVSARTAFLRRGLNNQLRLLYSVRTEVEEISLRGRSEFAAVLKDLDDADTRLRRTLELLRETIVHPAFRPEGEDPKSLHDFVDERGVEELHAALKSSIDRTTAAQAQLDSSNHAFDDELLSIKEALGTYRTAAKLASSRSSSSASSSSTSNSSLPSISTMPSMLHSLEMHAQEMANLLESLVRHFDLCVTAVKHTEGGGAAAKSITGDMPVGVPVSGRMGSNIEEEINNNLNAPLDPLSNSEYQEMVNVLFKDAAEAEDVVMEIQDRIGEMESVLENVLAQRDALRSIYNATTDIYQHLSSLGSTRLPGYIAQAHNFTQVWHEENDRISGGLADLSDLNSLYNGFLDAYDGLIVEVARRKHVRQRVEKVLRDTRHKLDQLYEEDVTARETFRVEKGDYE"
  },
  {
    "reference": "MSDSKLKKVLVGVGGGIAAYKVCEVVSTLFKAGMEVRVILTNSAQKFVAPLTFATLSRHRAYTDELFWRSSNPRPLHIELGEWADVFVIAPLTANTLAKLACGIADNLLTNTVLASKCPILLAPAMNTEMWEQHTVRDNWEKLLTDSRYHSMGTASGLLACDRIGAGRMAEPAEIVSHVYSLLHTAGERDLFGKQVLVSGGGTREYLDPVRYIGNPSTGKMGLALAQAALHRGAKVTLVHAPVTWELPLGIEAIPVVTSEEMQQAIMLYLQIADVIIMSAAVADVKPRQYSKHKLPKYRLPDSLALEPVTDIIAEIARRKQPRQKLVGFAAQTGDIVTPALEKLQRKSLDAIIANPIDQPDSGFGCDTNKAVFLDRSGQKIEIPPCSKLKMAHHIFDFL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The presence of Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.2. The desired protein should be able to perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO23. We require a protein that can specifically bind to the metabolites produced in the coenzyme A biosynthesis pathway.4. The protein design must be able to perform phosphopantothenoylcysteine decarboxylase activity in order to contribute to pantothenate catabolic process. The designed protein sequence is ",
    "response#1": "RIVLGVSGSIAAYKAVTLLRTLVQEGAEVSVAMTESATRFVSPLTFEVLSRHPVATSVFAAGQEMIHLTLPEHADVIVIAPATANQLAKYATGLADDLLSTMVLTTTGPLILAPAMDGGMWDHPAVQANVCTLRQRGVIVLDPGLAGRHVVVSAGGTREYLDPVRYLGNRSSGRQGYALARTAALRGASVTLVAANVSLPDPAGVKVTRVGSTRELRDAVLAAARDADAVVMAAAPADFRPAGYADAKIKKADDGSAPTVELTQNPDILAELSHDRPRPGLVVAGFAAETGDATAGVLDHGRAKLARKGCDLLVVNEVGVDKGFESPDNEAVIDALGHEVWVPRASKSELAARLLDLVTPRLQAR",
    "response#2": "KTIVLGVTGGIAVYKAVELLRLLTKAGATVHVVMTKAATEFVTPLTFQTLSGNPVHTELFNLISEQEIGHISLADRADLFIIAPATANFVGKVACGLADDLLSTTVMATKAPVLFSPAMNVNMYQNPLYKENEERLKRHGYLFVPAVKGMLACGWEGEGKLPAPEVIFEMVKLAEAELLAGKRILVTAGRTEEPLDPVRFISNRSTGKMGFALAEAAYLAGAEVTLISGPTELEPFDNFRYFSINTSEQMAERVNHEFDAHDILIMTAAVADFKPKSFSEKKIKKNETTMNLDLVVTTDILKEIGGRKGDKLLVGFALETHDALNNAEKKLKEKKLDLIVLNNPNEPGAGFGHDTNIVTLIDRAGNVTELPIMSKEEVAGRLLDEVCRLL",
    "response#3": "KHILLVIGGGIAVYKSLELIRRLRDRGAEVTPVLTRAGAEFVTPLSVSALAGRKVFSDLWDLTDEAEMGHIELSRAADLVVVAPATADLMAKMAGGHADDLASTLLMATDKRVLIAPAMNVRMWEHPATRRNAERLAGDGILTVGPNEGNMACGEYGPGRMAEPMEILGAIELAGRHVVVSAGGTREYLDPVRYLGNRSSGRQGYALARTAALRGASVTLVAANVSLPDPAGVKVTRVGSTRELRDAVLAAARDADAVVMAAAPADFRPAGYADAKIKKADDGSAPTVELTQNPDILAELSHDRPRPGLVVAGFAAETGDATAGVLDHGRAKLARKGCDLLVVNEVGVDKGFESPDNEAVIEAVLARL"
  },
  {
    "reference": "VQIFVRDNNVDQALKALK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein localizes to the ribonucleoprotein complex. The designed protein sequence is ",
    "response#1": "ETKIRLARAGF",
    "response#2": "MGTKIRLARAGR",
    "response#3": "MSTKIRLARAGR"
  },
  {
    "reference": "MNTQTALRLGVNIDHIATIRQARGTPYPDLVEAVRIVEEAGADIITLHLREDRRHIQDRDVEMLRERITTGMNLEMAATEEMQAIALRIRPQACCIVPERREELTTEGGLDVLGQEARLKAFCAPLLAAGIEVSLFVEPDLAHLDAALRIGAPVVELHTGAYASAAPGPDREAHLERIVAAAAHGHARGLVVNAGHGLDYENVLPIAAIPVMHELNIGHAIVARALFTGIGEAVRAMRAAMDRARR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino- 2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate..2. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate   = H(+) + 2 H2O + phosphate + pyridoxine 5'-phosphate.3. A protein should be able to manipulate the activity of enzymes in the pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 5/5, Cofactor biosynthesis pathway, either by activating or inhibiting their function.4. The 1-deoxy-D-xylulose 5-phosphate, 3-amino-2-oxopropyl phosphate binding site should be stable and able to withstand changes in environmental conditions.5. The protein should be optimized for stability and expression in cytoplasm where it will participate in pyridoxine biosynthetic process. The designed protein sequence is ",
    "response#1": "MSEKKEYSIQKALEEIEQGKMILVVDDEDRENEGDFICAAELITPEKINFMAIHGRGLICAPITEERASELNLELMVSKNTATHETPFTVSVDLLGHGCTTGISAFDRAHTIQALVNPNTKASDLGRPGHIFPLKAKDGGVLRRAGHTEAAVDLARLAGFEPGGVLVEIMNEDGNMARLPDLMKIAEKFDLKIISIKDLIKYRLQKDSLIEREIAIEMPTKHGRFMNIYTKKLGHMAHKEEPLETLKNQYNLRLEKDNKIGIYLHRENKKKKHDKRIELESNFNEYLKIEFDKIGFLLEENKTKENYIYQLEFNCGINAILKVNHEKSKFLEIMKILSNSIQEISNININKNLIIGFDIIGEKDLLEKLGIKEEKPLDINKNLYNLFCKNNLLIKNNLF",
    "response#2": "MPLDIAIATHNQGKIREIQAILAPLPVTLHTLADFPALPAVEETGATFEENARLKSRHAARLTGLAAIADDSGISVDALNGMPGVLSARWSGTHGDDAANTALLLAQLSDVPDERRGARFVCAAALALPDGTERVVEGRMTGTLLREPRGNNGFGYDPLFLPDDQPDGRSVAEMTPEEKNARSHRARAFRALAPRLLAAL",
    "response#3": "MPAHPSGRAHANRPLRDSEVAALLTELADWTIGAELAGVAVLPADDADLPLPGALAFLANPGYRAKLDRSRAGAVLVSQALAAEAGLDGPVIVSPDPRAALALLAARFYGHPAAALKVVAA"
  },
  {
    "reference": "MLSGKNILLGITGGIAAYKTTFLVRLLIKAGANVKVILTDSASSFVSPLTLSTLSKNPVLSSFVNKEDDTVSWNNHVELGLWADYMIVAPATANTLSKMANGTCDNLLLATYLSAKCPVFFAPAMDLDMYVHPSSKASFEKLQAYGNIMIPATSGELASGLHGEGRMAEPEAIVSFLKDYVVKGLPLRGKKVLVTAGPTYEAIDPVRFIGNHSSGLMGFEIAKAAANLGAEVILVSGPSHLKVSHDLIELIRVVSAEEMYLEVHKHFEKVTIAIAAAAVADYKPKTVATQKIKKKAEDISIELVKNKDILFSMGQQKTHQFLVGFALETENEIENAKGKLIRKNLDAIVLNSLNDSGAGFGKKTNKISFIDKNSTIKAFELKTKADVAKDIMNEIMNRLHA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should consider the endogenous proteins and enzymes present in the coenzyme A biosynthesis pathway to avoid interference with native metabolic processes.2. The designed protein must be optimized for phosphopantothenoylcysteine decarboxylase activity.3. The designed protein must have at least one Flavoprotein domain.4. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "LRDAGSDVRVVMTRGARAFVTPLTFQAVSGNPVRTELLDPDAEAGMGHIELARWADRILVAPATANFMARFAQGLADDLLSTLCLATTAPVALAPAMNRQMWAHPATQANCAVLEERGVRLIGPESGSQACGETGPGRMTEPAELVAATVSALGA",
    "response#2": "MDIVVGVTGGIAAYKAVQVVRLLVKAGHEVTVVPTPDALRFVGAPTWEAVSRHPVTTSVHDDVARVRHVALGQGADLVVIAPATANTIARMAAGIADNLLGTTLLATEAPVVIAPAMHAEMWRHPATQANIRTLVERGVRLVGPAAGELAGGDSGPGRMSEPEEIVDDALAAARRVLA",
    "response#3": "LRDAGSDVRVVMTRGARAFVTPLTFQAVSGNPVRTELLDPDAEAGMGHIELARWADRILVAPATANFMARFAQGLADDLLSTLCLATTAPVALAPAMNRQMWAHPATQANCAVLEERGVRLIGPESGSQACGETGPGRMTEPAELVAAIVAAVRT"
  },
  {
    "reference": "MVRIIPSRLKSSTSITSSTTNNGRSNSPPKSRAKDSMGPTCDNGLALKVSILKGRNLAAKDRSGTSDPYLVLVLGDAKAITHSVPKTLNPEWNVIEDLPVNSVQTLLLDVICWDKDRFGKDYMGEFDLALEEIFANGEPAQEPKWYPLRSKRRGKKSSVVSGEIQLQFTLHDTSNPSATAQQIFDKFNALVKAVPESTSRNITPTMTPSQGKSRSPKLGPTSGGLLAPTPATVPGEGLDTTLSEIEDDDDDDEEDDDLDVSDETPEEEDSTKPEAIERRKRRLRIKRLKRKRKENAYEFSGGSDVLGIIFLEICNITDLPPESNLTRTSFDMDPFVVASLGKKTYRTRHVRHNLNPVFNEKMIFQVLSHEQQYSFAFTVIDHDKYSGNDFIASCNLPVKDLIDKAPKANPETGLYDLKDPPEYEQPKRPRFRKLGLSRSSSAQSLSKLIKQSSSKAAADAGSATPSATSSSIAQAQPVVTPSGDSLAIPTPLSEGLETPPIETENQDFYEFSLPLKMKNLEKWEDKHNPVLFLRAKYMPYPALRQQFWRAMIKQYDADESGHISKVELTTMLDTLGSTLRESTIDSFFQRFPHRISGGEEQDLTVDEVVICLEEQLLANSNKQTTLGDRVRGLLRPTDSGSVTPHSESSASTPGVSEPLQPPLDQSHQDSSAPSTIVVPELNAPGEEGDFLSKDELVEDRGEEHVVEIRECPICHQPRLNKRRDTDIITHIATCASQDWRQVNKLTMGGFVTASQAQRKWYSKVITKI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The presence of pyruvate is crucial for the protein to function optimally under specific conditions.2. The protein should exhibit Basic and acidic residues compositional bias.3. For general function, the protein need meet that Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.4. The designed protein have EF-hand domains with specific sequence motifs that are crucial for its activity.5. The designed protein should be involved in protein autoprocessing by localizing to Golgi membrane and exhibiting calcium ion binding.6. The Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity of the protein must be engineered to enable effective catalysis of a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-   diacyl-sn-glycero-3-phosphoethanolamine + CO2. The designed protein sequence is ",
    "response#1": "VMLEIDSAANLPKLKNMTRTGWDMDPFVVISFGKKVFRTRVIRHSLNPVWDEKLLFHVRKYERSFSVAMTVCDWDKISSNDHIGDASFTLSKLLELGDARQSTPTIFKTLNPEWNVTFEMPVVGVPLLECICWDHDRFGKDYLGEFDIALEDIFSDGNIQQQPKWYTRKIVQMEIKSACDLPRWKNSLHTGFDMDPMCIVSFSDKIFRTRVQRHTLSPVWDEKLLFHVRRHEAQWKTKLSIFDWDKLSAHDHVGSTEISVADLIEEAPKPDIVQMEIKSACDLPRWKNSLHTGFDMDPMCIVSFSDKIFRTRVQRHTLSPVWDEKLLFHVRRHEAQWKTKLSIFDWDKLSAHDHVGSTEISVADLIEEAPKPDLPVALEFVVWDKDRWTANDYIGEVSLPTHSRLKVYVIKARNLAPKDRSGTSDPYLVVTLGNAKQSTPSISKNLNPEWKICFDMPLTGVPLLECMCWDKDRFGKDYMGEFDIPIEDIFTNGQIQTQPKWYKSAPLTIRGTDRKALRVVIMRARNLAAKDRSGTSDPYLVVTLGDARVVTHSVPKTLNPEWNVIEELPISSVQSLVVGVICWDKDRFGKDYLGEFDLALEEIFQNEVAEQTRPSPPRLGSNNNSTAALVDQKPLILKVYVIKARNLAPKDRSGTSDPYLVVTLGNAKQSTPSISKNLNPEWKICFDMPLTGVPLLECMCWDKDRFGKDYMGEFDIPIEDIFTNGQIQTQPKW",
    "response#2": "MRTPDGKPLVLKVSVLRGRNLAAKDRGGTSDPYLIVTLGEARQSTPTIFKTLNPEWNVTFEMPVVGVPLLECICWDHDRFGKDYLGEFDIALEDIFADGDVQQQPKWSLAARAYQFSGLGNGVQGIVFMEIVKVTDLPPERNVTRTSFDMDPFVVTSLGRKTLRTPVIRHNLNPVYNEKMVFQVMKHEQLYTMSFTVMDRDKFSGNDFVASAGFALQTLIQAAPDTDPRRQSNDVAQFYSDVFGAVYIEVMNARDLPPERNMTRTGFDMDPFVVISYGKTTFRTSSVRHNLNPDWNEKLMFHVRANETRYSIKFAVYDKDKFTKNDHVAWAEVPIMDIIKQPHAIDMTL",
    "response#3": "MRTPDGKPLVLKVSVLRGRNLAAKDRGGTSDPYLIVTLGEARQSTPTIFKTLNPEWNVTFEMPVVGVPLLECICWDHDRFGKDYLGEFDIALEDIFADGDVQQQPKWKPTSPVPSRSGSPKLMNESSLVLKATVLRGRDLAPKDKNGFSDPYLVVSLGEYRFQTEAMPKTLDPEWFDNFEIPLSGANSSTLECVCWDKDRFGKDYMGELTVHLDEFFSNGRTSQEPRWRRQSNDVAQFYSDVFGAVYIEVMNARDLPPERNMTRTGFDMDPFVVISYGKTTFRTSSVRHNLNPDWNEKLMFHVRANETRYSIKFAVYDKDKFTKNDHVAWAEVPIMDIIKQPHAIDMTKTGGDKKGHHYELSRESDVVGVVFLEINSITDLPPEKNMTRTGFDMDPFVVVSLGKKTYRTRHIRHCLNPVYDEKMVFQVLRHEQRYSLNIAVVDRDKFSSNDFVAQTNFALQEIISTQPTADPVLKVSVLRGRNLAAKDRGGTSDPYLIVTLGEARQSTPTIFKTLNPEWNVTFEMPVVGVPLLECICWDHDRFGKDYLGEFDIALEDIFADGDVQQLLRVQILCCEDLLPKDRGGTSDPFVVVSLLGNKQQTSVVKKTLNPTWSAKDATLDLPIYLSLADKLGALELLVWDKDMLRKDYLGEHALPLEDWFKGTAYSFGFTVHIPSSEDTQTTATTTTTAPRLVLRISVLRGRQLHAFEDTAPNSFAVVRCAGQQQRTHVVKKSSEPEYHCTFDVRLSGNRSRGTRRRLRLLLSRGLVISVCDRDRFKSVYLGQVRLPADELFGETDPIAYD"
  },
  {
    "reference": "MFKLAKIMIQGTGSSVGKSILVTALCRIFKQDGYTVCPYKSQNMALNSYITYDGKEMGRAQVLQAHAAGLEPEAYMNPILLKPTGDKKCQIIFNGEVYGNSTAMEYHNMKTKFGEKLKDQFKNIEEKFDIVVIEGAGSPAEINLRDKDIVNMGFAEMVDAPVLLAGDIDRGGVFASLAGTMLLLTEEERNRVKGTIINKFRGDIEILKPGLKMLEDIIKIPTVGVVPYLKFQLEDEDGAIDFNRNINAPIDIAVIKLPRISNFTDFDALKVEEDVSIRYITSKEDFGKPDLLIVPGSKNTIEDLLALRKCGLEEKIKEYSKTGTIIGICGGYQMLGKTLKDPYKVESEELETQGMGLLNVDTVFEKEKVTTRVKANSEDTEVYGYEIHMGICNYKEGAKPLFNIYDENGKKVNRNDGAINEKGNVMGTYIHGVFDGVEFREKVLNNIRKSKNMEERNAINYENLREKNLDMLADLVRENIDMDYIYKIIGM",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide; NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation..2. The protein must have high specificity towards ABC-type vitamin B12 transporter activity, catalytic activity.3. The designed protein must have a active site active site that is highly conserved among related enzymes.4. Ideal protein should have a binding affinity for key enzymes in the adenosylcobalamin biosynthesis, Cofactor biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "RVAVIRLPRLSNVTDIDALRVEPGVAVRLATRPDELADADLVILPGTRSTVEDLRWLRRRGLAAALAERAAAARPVLGICGGYQILGRRIRDDVESGAGEVDGLGLLPVITTFDPVKLLGRRAATDAAGRPLTGYEIRHGRLTVEEHPDSAPFAADGVRVGAVAGTSWHGVLENDPAWLAPFLARIAVVAYPRISNLDEFQPLKNVPGVRLSWARSPADVEGADWIVLPGSKATAADLAWLRAQGLDAAIAAHAARGGRVLGVCGGLQMLGEALIDTVGVDGNGPGLGLLPLVTSFEATKTVRPTRQCFGAVQGAWRHLAGVAVQGYEIHHGQTAQHPAMAASGDVARELMPGLAWQNPAGNVLGLYLHGLFEDPAWLAPFLAG",
    "response#2": "IAVIRLPRISNFTDFEPLAMEPDVRVEFVDPRDNLPEDADAVILPGTRTTISDLEELRKRGMDEEVVQAADEGTVVLGVCGGYQMLGRELVDESGGELDPGESVPGLGLLDAVTVFPSDAGKVTVRSEGVVNHPHLRGIRVEGFEIHEGRTYTDEPHLVRLRSGYGNRGCFLDGAYRTDRPVLGTYLHGIFFNRRFRHEFS",
    "response#3": "IAVIRLPRISNYTDFHNLERFPNVSVRYISKVSELLEPDMIILPGTKNTIDDLKFLRESGLEAAILKAESKDTLIFGICGGYQMLGEWLHDPYGVEGGGEIKGLSLLPINTVFAKEKVRKQNSGTLNKVAGMLSGLSGKTYQGYEIHMGESTWSSKVEEEKNNCFSNKDEENLEHNTITSVLISNGAHVYGTYLHGIFDEEGICKEILISLGE"
  },
  {
    "reference": "MSEEEGRERAVTVEGDAESMNDGRALVQPPARSGDVITPTRAVLTLSKSMFNAGCFSLPYAWKLGGLWVSFVMSFVIAGLNWYGNHILVRASQHLAKKSDRSALDYGHFAKKVCDYSDIRFLRNNSKAVMYFVNVTILFYQLGMCSVAILFISDNLVNLVGDHLGGTRHQQMILMATVSLFFILLTNMFTEMRIVSFFALVSSVFFVIGAAVIMQYTVQQPNQWDKLPAATNFTGTITMIGMSMYAFEGQTMILPIENKLDNPAAFLAPFGVLSTTMIICTAFMTALGFFGYTGFGDSIAPTITTNVPKEGLYSTVNVFLMLQSLLGNSIAMYVVYDMFFNGFRRKFGARFPNVPKWLSDKGFRVFWVLVTYLMAVLIPKLEIMIPLVGVTSGALCALIFPPFFEMITFWTDWKGLLTYRQRMTKIFINLVVMAIGVFAIIAGVYTNIHAIIQSFSQPDP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Plays a role in the accumulation of vital dyes and endogenous fluorescent compounds in lysosome related organelles2. The protein should be able to modulate amino acid transmembrane transport in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "FHPLFMALGLQFLFGEAILIFRMFPFTNKKLLKIGHGVLHASAMVLFAFALIAAFRSHSTAAIPDLYTFHSWIGIALVACYVLQFLFGLVIFFLPVIGESIRKMYLPIHKVAGIIMFIVSSATCLTGQ",
    "response#2": "PNGLNLTEAWQDLQSLTKGFHPYNSHQNDEVRSWLLERIDAIKQSTPSTEEYRDAKEEKPDVFVFDDLVSNLTFIDKSVGVYFEGTNILVYIRGSEDNKQNWWETPGRAPVGKGGVLVNAHYDSVSTGYGATDDGVGVVTCLQLVKYFLTPGHAPRRGLVVLFNNGEEDYLNGARVYSQHPMARFAHTFLNLEGAGAGGRATLFRSSDTEVTQAYAKSEHPFGSVLSANGFEKGLISSQTDYVVLEGILGLRGLDVAFFEPRARYHTDQDDARHTSIDSLWHMLSTAVATTEELVSDTTDRFDGHIRDDGTVPSGSGTRAVWFDLFGSAFAVL",
    "response#3": "PGRAPVGKGGVLVNAHYDSVSTGYGATDDGVGVVTCLQLVKYFLTPGHAPRRGLVVLFNNGEEDYLNGARVYSQHPMARFAHTFLNLEGAGAGGRATLFRSSDTEVTQAYAKSEHPFGSVLSANGFEKGLISSQTDYVVLEGILGLRGLDVAFFEPRARYHTDQDDARHTSIDSLWHMLSTAVL"
  },
  {
    "reference": "MENIVQSKPIIELRSLKKSYNENTIIDNFNLTINNGEFLTILGPSGCGKTTVLRLIAGFEEANGGQIILDGEDVTDLPAEHRPVNTVFQSYALFPHMTIFENVAFGLRMQKVPNEEIKPRVLEALRMVQLEEMADRKPTQLSGGQQQRIAIARAVVNKPKVLLLDESLSALDYKLRKQMQNELKALQRKLGITFIFVTHDQEEALTMSDRIIVLRKGNIEQDGSPREIYEEPSNLFVAKFIGEINIFDAQVLNRVDEKRVRANVEGRVCDIYTDLAVKEGQKLKVLLRPEDVQLEELDENEQSSAIIGHIRERNYKGMTLESTVELEHNNKLVLVSEFFNEDDPNIDHSLDQRVGVTWIEKWEVVLNDENDNA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit ATP binding.2. The protein must exhibit the following characteristics: Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. The designed protein sequence is ",
    "response#1": "EGKFYTLLGASGSGKSTILNLIAGLLEASSGGIFLDGKRINDIPINKRDVHTVFQSYALFPHMTVFENVAFPLKLRKVAKKEIEKRVTEALNMVRLAGFEKRSIGKLSGGQRQRVAIARAIINQPKVVLLDEPLSALDLKLRTEMQYELRDLQQRLGITFVFVTHDQEEALAMSDWIFVMNDGR",
    "response#2": "EGKFYTLLGASGSGKSTILNLIAGLLEASSGGIFLDGKRINDIPINKRDVHTVFQSYALFPHMTVFENVAFPLKLRKVAKKEIEKRVTEALNMVRLAGFEKRSIGKLSGGQRQRVAIARAIINQPKVVLLDEPLSALDLKLRTEMQYELRDLQQRLGITFVFVTHDQEEALAMSDWIFVMNDGR",
    "response#3": "EGKFYTLLGASGSGKSTILNLIAGLLEASSGGIFLDGKRINDIPINKRDVHTVFQSYALFPHMTVFENVAFPLKLRKVAKKEIEKRVTEALNMVRLAGFEKRSIGKLSGGQRQRVAIARAIINQPKVVLLDEPLSALDLKLRTEMQYELRDLQQRLGITFVFVTHDQEEALAMSDWIFVMNDGQ"
  },
  {
    "reference": "MTKFIFVTGGVVSSLGKGITAASLGRLLKNRGLTVTIQKFDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLSQNSNVTTGKVYSAVINKERRGDYLGGTVQVIPHITNEIKDRVFRAAREARPDVVITEIGGTVGDIESLPFLEAIRQIKTEVGRDHVMYIHCTLVPELSATGELKTKPTQHSVKELRGIGIQPNVIVCRTERKLSEDIRRKLALFCDIDPDAVIECRDAETLYEVPLMLQSEGLDRIVAKHLDLDGGEADMKEWIDLVDQVKNLTHRTKIAIVGKYVALRDAYMSVVESLNHAGFYNDTEVDLMWVNAEEVNDTNVSEVLKDADGILVPGGFGDRGIEGKIAAIRFAREQRIPMFGICLGMQVACVEGARNLLGLEGAHSSEFDPETPHPVIDLLPEQKDIEDLGGTMRLGLYPCKLAPESRAQEAYGNGLVYERHRHRYEFNNEYREKLAEAGYVFSGTSPNGRLVEIVEWKDHPWFLGCQFHPEFTSRPNRPQPLFRDFVAAALSVKLKTAQV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile, Nucleophile; for glutamine hydrolysis, active site to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).2. The UTP, ATP, CTP, L-glutamine, Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.3. A protein with glutaminase activity, CTP synthase activity, ATP binding functions is required to enable glutamine metabolic process, 'de novo' CTP biosynthetic process.4. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen5. A protein that is capable of catalyzing reactions within the CTP from UDP: step 2/2, Pyrimidine metabolism, CTP biosynthesis via de novo pathway pathway with high efficiency and specificity.6. The designed protein have Glutamine amidotransferase, CTP synthase N-terminal domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "KYIFITGGVCSSLGKGIAASSIGAILEAMCYKVAMQKIDPYLNVDAGTMSPYQHGEVYVTDDGAETDLDLGNYARFTDSELSKLSSITTGKVYLNVIERERRGDYLGKTVQVIPHVTSEIQRLIRSLSDKVNPDVQIVEIGGTVGDIESIPFLEAAREFSLSNKNNTIFIHLTLIPKIEVAGEYKTKPTQHSVGVLRQIGIVPDIIMTRISGTLSKEMKRKIAMFCSIAEEDVFEAPDVDSIYKLPIIYTQQGLQDKIALKLSLQDSYKSVYEALRHAAIANKVELNIMKIDSEEIEKNPENLTKVINQADGILVPGGFGSRGIEGKLAIIQIAREKKIPYFGICLGMQCMVIEYARNVLKIADANSSEFTETSNPVIHLMEDQYSIDRKGGTMRLGVYECNIKKGTLAEKIYGENIIFERHRHRYEFNNKYEDQFEKAGLIISGKYITKNLVEIVEYKDNPFGIGVQFHPEFKSKPFKPHPIFKSFIDKINNK",
    "response#2": "QYIFVAGGVMSGVGKGIASASIGKILQSRGFSVTAIKIDPYVNVDAGTMNPTEHGEVFVLDDGMECDQDMGNYERFLDVNLSSDNYMTTGSVYQAVINRERALEYKGKCVEVVPHIPMEVINKIKKAAKKNRAEIVITEIGGTVGEYQNILFLEAVRMLKAENPGDVLLVLVSYLPIIGDGHELKTKPTQYAVRTLNSAGLQPDIIIARSKNGIDKKRKEKIAISCGVEENGIISAPNVESIYDIPVNFEKDNLSDFIIKKLKLQDSYKSVYEALRHAAIANKVELNIMKIDSEEIEKNPENLTKVINQADGILVPGGFGSRGIEGKLAIIQIAREKKIPYFGICLGMQCMVIEYARNVLKIADANSSEFTETSNPVIHLMEDQYSIDRKGGTMRLGVYECNIKKGTLAEKIYGENIIFERHRHRYEFNNKYEDQFEKAGLIISGKYITKNLVEIVEYKDNPFGIGVQFHPEFKSKPFKPHPIFKSFIKETIEAKKIKDSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASFVVKIGIVGKYTKLHDAYVSIREAFIHAGAALDTKVEIEWIESEGFENDPESVSVLKDYDGILVPGGFGSRGIEGKINAIKYVRENKIPYFGICLGMQMMVVEYARNVCGLKGANSTEFDENTKSPVIDLLPEQRAIKDKGATMRLGAWKCELAKGTRAFEAYGKQSIEERHRHRYEVNNDYLETLEKSGIVLSGRSGHIVEMVEWKEPHGFGIGLQAHPEYKSRLEEPAPLFVAF",
    "response#3": "KYIFVTGGVVSSLGKGIAAASIGCLLESRGLRVTLQKFDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTYAPLTQSNNVTSGRIYETIISRERRGDYLGKTVQVIPHVTDEIKANIEKVSGGVDVVLVEIGGTVGDIESLPFLEAIRQLRHKKGRENCFFVHVTLVPWIAAAGELKTKPTQHSVKELRTIGIQPDMLICRCEKPLAGDIKDKIALFCDVSADAVIEACDAKSVYEVPLGFASQNVDDIILRQFKLDVKEADLKEWEEVVIGMVGKYVELPDAYKSVNEALKHAGLKNRLSIKIQYVDSQDLEVKGTDVLQHLDAILVPGGFGERGVEGKILAAQYARENKVPYLGICLGMQVALIEYARNVAGLKGAHSSEFDAKSKYPVVGLITEWIDEDGKVETRDVDSDLGGTMRLGSQLCHLTKGSKVADLYGSVEIYERHRHRYEVNNTILPKITAKGLQVTGLSSDKKLVEIIENPAHPWFVAAQFHPEFTSTPRDGHPLFEGFVKAALERKEK"
  },
  {
    "reference": "MSMQDPIADMITCIRNGQFSNKIFVIVPFSRLKENIVKVLKFEGYIINYKIKKNNNKVLKIFLKYFNGKSVIENIKRISRPSLRRYCNKRNLPIVMNNLGIAIISTSRGVMTDRIAREKGLGGEIICYVD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should be able to enhance the efficiency of translation. The designed protein sequence is ",
    "response#1": "YDCYANLALLKLYQFNPHLSRDETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAKGTVVREL",
    "response#2": "ADVGLVGLPNAGKSTFLKATTNAHPKIGNYPFTTLHPQLGVVRRHGQEFVLADIPGLIEGASEGIGIGDRFLGHIERCRILLHLIDASGEDPIAAWHEVQNELALYGAGLAEKPQLLALNKIDSVDEETCAELSQALEEASGQKVLLLSGATGQGLDPILDQI",
    "response#3": "YDCYANLALLKLYQFNPHLSRDETVTNILVKALTMFPSPDFALGLSLLPSHLLAPLNSSAHNPAAGDAPLSEAVQKLNELRNLLEGADYATFWSTLDSDDLYADLIADVSGFEELMRVRIAATVSQAVREVDRSILESWLNLEGSDFEHFVGSVCGWTIEGAKIKVPMNKDNEAKGTVVREL"
  },
  {
    "reference": "MNLYEYLSQGIKHFTLIDPDKSVDYLRIARYALEAGTDGILVGGSLGITASQMEKVVKDIKSVANVPVVLFPGSLSQLTDAADGVLFLSVLNSLDPYFIIGAQVHGAVLIAKHYPRLEVISTAYVIVGDGGAAGFVSMSKPIPYTRPDIATAYALAAGYIGFKALYLEAGSGAPQPVPPDMVRAVRKAFPRVLIVGGGIRSSEVARSIAREGPNVIVTGTLAEESPEKLGEIVRAIKQR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be designed to have high stability and activity towards (2E,6E,10E)-geranylgeranyl diphosphate + sn-glycerol 1-   phosphate = diphosphate + sn-3-O-(geranylgeranyl)glycerol 1-   phosphate.2. The protein design should consider the endogenous proteins and enzymes present in the glycerophospholipid metabolism, Membrane lipid metabolism pathway to avoid interference with native metabolic processes.3. The protein must exhibit the following characteristics: Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P); This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids..4. The protein requires Mg(2+) to activate a critical catalytic site.5. The protein should have a high affinity for sn-glycerol 1-phosphate, Mg(2+).6. A protein that can perform magnesium ion binding, phosphoglycerol geranylgeranyltransferase activity in cytoplasm to support glycerophospholipid biosynthetic process. The designed protein sequence is ",
    "response#1": "MSNTPVKSTIVIGSGPAGYVAAIRSAQLGLKTVCVEKYGSLGGTCLNVGCIPSKALIEASEQYHKAKEGFDELGIKSEGIQLNIEKMQQWKKGIVKKLTGGIAHLFKKNKVHTILGEAKFTSKNELIVETNQGQKKITADNIIIATGSRPIEIPGFEIDGERIMSSTGALELQEIPASMIVVGGGIIGLEMATVWNALGTKVTVIEALPQILPGADRDLIKVLQKQMKKQGMEILTGVKVSKIEKKGANLIVHYTNNQKETIEADKVLVAVGRVANTEGLNLEAAGIKIDSKGFIQVDQYMRTSNEGIYAIGDVIGQPMLAHKASHEGIIAAESIAGANNHIDYNAIPGVIYTSPEVASVGLTEEQAKEAGIDVKVGKFPFSANGRALANGETEGFVKVIADAKYGEILGVHIIGPHVTDLIAEGSMAIELEATAEDVIRTIHPHPTLSETIMEAAANVHSKAIHN",
    "response#2": "MSDEEPTTQQDDLDSISLFGNNGKKRSSSTSSVNNNNNNKPNNNNNNNNKQHTNNNDDDDDDDDDGDEDEDEDDDDEDDNDDDDEDDDDEDEEDVVSKPNQPKNKNKKQQQKQKQQKQKQKQKQKQQKQKQQKQKQQQKKQPKQKQQQKQQQQQEEEEEEEQQQQQQKQQSKKKKSNNNNNNNIKTNNNNQIKQQQIQSIKNNNNNNNNNNGNNGDNGNNNNENGNENGNINNNNNNNNQNQNNNNNNNFNNNNNNNNNQQQQQQQSSSSQQSSGSSSNKSSSSGGGYNWNGGINSNNNNNNNNNNNNSNNNNNNGNGNSSNSSNSNFSNKNNNNNNNNSTNNSNSSSNSSNNNNNSSNSSNNNNNSSSSNNNSNSNSNSSNSSNSSNSSSNSGSSNNNNSNSNSNSNSSNSSNSSNSSNSNNSSSSSNNNSSNSSNSSSSNSSNSSNSSNSNSSNSSGNNNSSNSSNSSNSSNSSNSSNNSNSSNSSNSSNSSNSSNSSNSSNSSSSSSNSSNSSNGSGSGGNSSNSNNNNGSNSSSNQNNNNNNSNGNSSQNNSSSSEQNNSSSSSNNNSNSSSQQNNNNNSSSQQQNNSSSSQQQNNSSSSQQQNNSSSSQQNNSSSSQQNNSSSSQQQNNSSSSQQQNNSSSSQQQNNSSSSQQQNNSSSQQQNNSSSSQQNNSSSSQQQNNSSSSQQNNSSSSQQQNNSSSSQQNNSSSSQQQNNSSSSQQQNNSSSSQQQNNSSSSQQQNNSSSSQQNNSSSS",
    "response#3": "MPHPNRSIRTAARNTHIARTSFRPTPRPSQSQAYQSSLSRRPATHTRTYATSAEAAAAAPPAPKPWRISNLALLRPYTFASSVPNIVLGTTFTALLLSPTRSTPSSNPWTAFILSSLSPTLISVYLLGYLTNSMSDFDLDVQIKKNRPIPSGAVRIRDAVVLYAGLTVAGLLGAWYRGSGLATLAWFYAVNVAYSAYPFNLKATPFPLFLLCFSLFHSTPALFSSSPTLIPHLSSPALLTSSFTTLLISTLSLFKDMHDIDGDRRNGIKSLPILLGERSTRLASAVFALSWTGAIAVGALAGGWGWGVWGVVGGVLGNGVMWRLLGVDVVGDGRGMAEWWGWGVWGVWGVWVVMGVLGV"
  },
  {
    "reference": "MRPLTMSGHFLLAPIPESSSDYLLPKDIKLAVLGASCVGKSAMIVRFLTKRFIGDYEPNTGKLYSRLVYVEGDQLSLQIQDTPGGIQAQDSLGQMVDSLSKCVQWAEGFLLVYSITDYESYQSIRPLYQHIRKVHPDGKAPVVIVGNKGDLLHARQVQTHEGLQLANELGSLFLEISTSENYEDVCDVFQHLCKEVSKLHSLSGERRRASIIPRPRSPNMQDLKRRFRQALSSKAKAASTLG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The GTP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "LRDAYLSVAEALGHGGIENDVRVEISWIDSEEVSDENAEEYLGKCDGILVPGGFGDRGIEGKISAARYAREHKIPFFGICLGMQMAVVEFARHVAGFSDAHSSEFNEHTLHPVIYLMPEQRGITEKGGTMRLGSYPCDLETDSRAYEAYQTPQIQERHRHRYEFNNDYREQLTAKGLKIAGLSPSRHLVEIVELADHPWFVGVQFHPEFKSRPNRAHPLFREFIASM",
    "response#2": "LRDAYLSVAEALGHGGIENDVRVEISWIDSEEVSDENAEEYLGKCDGILVPGGFGDRGIEGKISAARYAREHKIPFFGICLGMQMAVVEFARHVAGFSDAHSSEFNEHTLHPVIYLMPEQRGITEKGGTMRLGSYPCDLETDSRAYEAYQTPQIQERHRHRYEFNNDYREQLTAKGLKIAGLSPSRHLVEIVELADHPWFVGVQFHPEFKSRPNRAHPLFREFIAN",
    "response#3": "LRDAYLSVAEALGHGGIENDVRVEISWIDSEEVSDENAEEYLGKCDGILVPGGFGDRGIEGKISAARYAREHKIPFFGICLGMQMAVVEFARHVAGFSDAHSSEFNEHTLHPVIYLMPEQRGITEKGGTMRLGSYPCDLETDSRAYEAYQTPQIQERHRHRYEFNNDYREQLTAKGLKIAGLSPSRHLVEIVELADHPWFVGVQFHPEFKSRPNRAHPLFREFIALSTR"
  },
  {
    "reference": "MGGKWSKCSPVGWSEIRDRIKRTPPAAEGVGAASRDLERHGALTTSNTPSNNATCAWLEAQEEEGDVGFPVRPQVPLRPMTYKAAFDLSFFLKEKGGLEGLIYSKQRQDILDLWVYHTQGFFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPKEVEEDNKDENNSLLHPVSLHGVEDEDKEVLMWKFDSHLARRHMARELHPEYYKDC",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. Incorporating a Dileucine internalization motif; necessary for CD4 internalization motif is critical for the successful design of the protein.2. The designed protein should be involved in suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host autophagy, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II by localizing to host cell Golgi membrane, virion component, membrane, host cell plasma membrane, extracellular region and exhibiting GTP binding, SH3 domain binding.3. For general function, the protein need meet that Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD. The designed protein sequence is ",
    "response#1": "MGGKWSKRSFPGWPAIRERMRRAEPAAEPAAEGVGAASQDLARHGALTSSNTVANNAACAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLVYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPDQVEKATEGENNSLLHPICQHGMDDPEKEVLVWRFNSRLAFEHKAREIHPEYYKNC",
    "response#2": "MGGKWSKSSLVGWPAVRERIRRTEPAAEGVGAASQDLDKYGALTSSNTDTNNAACAWLQAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVDPDKVEEANEGENNCLLHPMSQHGMDDPEKEVLMWKFDSRLAFHHMAREKHPEYYKDC",
    "response#3": "MGGKWSKRSVPGWPAVRERMRRAEPAAEPAADGVGAASRDLDKYGALTSSNTATTNAACAWLEAQEDEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLVYSQRRQDILDLWIYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPDKVEEANEGENNTLLHPMSQHGMDDPEKEVLMWKFDSRLAFHHVARELHPEYYKNC"
  },
  {
    "reference": "MTFLQYFKTDGIRGKVGVNPITPDFLLKLGWSIGIVLGKNKTQKIIIGRDTRISGTMLQSILEFGILSTGVSTLLAGCMPTSAISYFTKSLNASAGIVISGSHNPFYDNGIKIFYKNGVKLTKEIEFSIEQKVQHTFLYPDYVNFGHSNNILDPESLYIDFCKKNFPKDLNLSKFTIILDCANGATFKIAPKIFEDLGARVITVAINPNGVNINQNSGSTNILMLKKIVLSESADLGLAFDGDGDRVIMVDHLGNQVDGDQIIYIIAKEYLKENKLKGGVVGTSMTNMGVILGLKKLGIPFCPAQIGDRNVYEKIKEKKWILGAEKSGHIVLLDKHSTGDGIIASLQVLLTMINNHMTLYDLSNQIKLFPQVFLNIFLKQDKDFEKDIKIQNILTQYKNILGQNSRVLVRRSGTEPCIRIMVEGEDYLKVYELAQYIGKTIKLL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design must be focused on enhancing the efficiency of carbohydrate metabolic process.2. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.3. The protein should be designed to catalyze the following reaction alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate. The designed protein sequence is ",
    "response#1": "DGDEALCIIALARAGEGALGGGVVGTQMSNLGLELALKAQGIAFERAAVGDRYVMEMLQQRGWLLGGESSGHILCLDRTSTGDGIVSALQVLAIMQATGRPLSELRKVMAV",
    "response#2": "DGDQLLGALGAFMSDRGTLKGGGIVSTVMSNRGLDDFMSDKGLKLFRSDVGDKNVLEVMKKEGINFGGEQSGHVIISDFAKTGDGLVSALQVLALLLKTSQKASKAD",
    "response#3": "NGDELLYLMTLDRLAAEPSEDAAPSRVGVPGVVGTLMTNLAVEQALAARGVAFVRAKVGDRYVLEELLARGWQLGGEGSGHLIALDKHTTGDGLVSALQVLQAVVRSGRTLAQLLEA"
  },
  {
    "reference": "MSITPSPRVGIIVVAAGSGTRLGAGEPKALVGIDGSSILHHALRRVLRAPSAQIVIVAPSGREGDALTEALAVADAMDAVTPPPSGRRRDLVHVVVGGATRQESVAAGLAALWADVEVVLVHDAARALTPPAVFERVVAAVEETASGVVPVLPVIDTLKRVEGTAVVEAVDREPLAAAQTPQGFPRAMLVAAYRAATREYTDDTALVAAQGHAVSTVEGDARAFKITTPADLERARALVTDAEPGAEPEPPVALEAAIAAPRVGIGTDVHGFGGEGDLWLAGLRWPGEQALSGHSDGDAVAHAIVDAVLSAAGLGDIGTHFGTSRPEFTGAHAEAFLARTRALVGEAGWRIGNVTVQVQANRPRFAGRRAEAEQVLSAALGAPVAVSATTTDGLGFTGRGEGVAAFAVALLLPV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should take into account the physiological conditions of the isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6, isopentenyl diphosphate biosynthesis via DXP pathway pathway.2. The protein must exhibit the following characteristics: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D- erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C- methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF)..3. The protein contains novel 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domains that confer a unique function or activity.4. The designed protein must possess 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity to support terpenoid biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "MADERLSRQEQRSPLPDVRTGNGYDVHQLVPGDGVTLCGVFIPHDQKLSGHSDADVALHALTDALLATCGAGDIGDHFPPSDMQWKGAPSRIFLEHAARIVREHGGTIMNADVSLIAEAPKVGPHRETMRRNLSEFLDIDMERCSVKATTNETIGFVGRREGIAAIATATVEALSPDLIALAAAQRQVTFTDDASIAEWAGMRVALAMGSERNLKITSREDMALAESLIAQGAETRVGTGFDVHAFGPGDTVWLCGVPIPHERGLVGHSDADVGLHALTDAILGALAAGDIGAHFPPSDPQWRAAASHIFLERAADEVERRGGRIVNADVTLICEAPKIGPHRQAMRSAIAEILRVPVRRISVKATTSEKLGFTGRREGIAAMATASE",
    "response#2": "MADERLSRQEQRSPLPDVRTGNGYDVHQLVPGDGVTLCGVFIPHDQKLSGHSDADVALHALTDALLATCGAGDIGDHFPPSDMQWKGAPSRIFLEHAARIVREHGGTIMNADVSLIAEAPKVGPHRETMRRNLSEFLDIDMERCSVKATTNETIGFVGRREGIAAIATATVLVESGAEVLRHPEDFAAAEARVTWETRSASGFDVHRLEAGEELWLGGVLIPHDKGLSGHSDADVGLHALTDALLGTIAAGDIGTHFPPSDPQWKGADSAQFLQHAARLIADKGGRIDFVDLTLICEAPKIGPHRAAMRARIGALLGLSEDRVSIKATTTERLGFTGRGEGIAAQAVATVLS",
    "response#3": "MMLAQKCLAQPRTALGTDVHAFGPAGSGPLVLGDVEIPHDRGLVGHSDADAALHAVVDALLGLACAGDIGVHFPPTDPQWRGVASRVFVEKAVGILGTAGGRLTHLDLTIVCEAPRIGPHRQAIRAALAEMVDLDAGLVSVKATTTEGLGFTGRGEGLAAQAVARAGNGFDVHRFGPGDAVTLCGVSIPHGRGLQGHSDADVGMHAVTDAIYGALGMGDIGQHFPPSDPQWKGAASHIFLDHAVALARARGFALTHADVTLICEHPKIGPHAPAMKAEMARIMGLEPDRVSVKATTSERLGFTGREEGIAALATATLVRL"
  },
  {
    "reference": "MRNFASLGRLLFPLRSLSTSPVSASIKMGRRWKKKRPGHGGPGQRRDRPHRDQRQQRPNEWQDTFVKENKDFERFYKGQGIVPEGEWDQFLEGLRSPLPATFRITGYKSHAQELLRRVKGQYISQLLDVTSEDGELLEKPQPIPWYPDELAWRTNYSRKDLRRSPALAKFHDFLVTETETGNVSRQEAVSMIPPLLLDVQPHHKILDMCAAPGSKTAQLIEMLHTEDGQSFPGGFVVANDADNKRCYMLVHQAKRLNSPCFLVTNHDASHMPNMTVPSVIYMIVDDYRMLLMVLQTPWPMTGYFVMYPVGRVQYAVFLGIARWCTTYKEKKSMMGPHVMTPLPNHHFLLCFAQGARIEFAPVRSGSDSVFRCWKIIKWWSYEKNHFRRTMPPIGGDGTMRKNPDVWKKWSPGQGQNLHNLQLRIVRRGIELLRVGGQLVYSTCSLNPLEDEAVIAALLTECEDSVELVDVSDKLAGLKRLSGVSAWKSGDFYSSISEVPTDRHSNLRRASMFPPPDPDKLQAMQLNRCIRILPHHQDTGGFFVAVLRKTKALPWTRQGKADRRKEETAETDEAKQPDTSATPVPAEEGDVSNETGAAAKDDGEGSATGQGEEADGKGSDTGQGKEDDGKKEAEAGNSNGSDGLARPPPAKKAKLAWEGSYKEDPFVFFTEDEELWPIMRDFYQISDTFPHLQLLVRCYEGKKRTVYLVSKEVKDILQYNENNLKVINCGVRVMVRSRNDKSKTMSLFRLAQDGIPTLLPYIQGRRVEI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The Nucleophile of the protein must be engineered to enable effective catalysis of cytidine(50) in tRNA + S-adenosyl-L-methionine = 5-   methylcytidine(50) in tRNA + H(+) + S-adenosyl-L-homocysteine.2. The designed protein must have at least one SAM-dependent MTase RsmB/NOP-type domain.3. A protein that can perform tRNA (cytosine-5-)-methyltransferase activity in nucleus, cytoplasm to support RNA methylation, tRNA methylation.4. The protein should exhibit Acidic residues, Basic and acidic residues compositional bias.5. The S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MEALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPQLEKFHQFLVSETESGNISRQEAVSMIPPLLLNAHPHHKILDMCAAPGSKTTQLIEMLHADMSVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLKIDVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLVEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSSELPGLKWMPGIAHWKVMTKDGQWFSEWEEVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKES",
    "response#2": "MEALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPQLEKFHQFLVSETESGNISRQEAVSMIPPLLLNAHPHHKILDMCAAPGSKTTQLIEMLHADMSVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLKIDVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLVEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSSELPGLKWMPGIAHWKVMTKDGQWFSEWEEVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKPE",
    "response#3": "MEALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPQLEKFHQFLVSETESGNISRQEAVSMIPPLLLNAHPHHKILDMCAAPGSKTTQLIEMLHADMSVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLKIDVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLVEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSSELPGLKWMPGIAHWKVMTKDGQWFSEWEEVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKS"
  },
  {
    "reference": "MKFVVKLFPEITIKSKPVRQRLVKQLRQNISAVLKKEFEDVKVQGFWDKIEVNLNDDSQEKAENLAATLQRIPGIANILRVQRYQIDNIDDNFDEIVEHTHRALGEELKGKRFVVRIKRAGQHSFTSHELERYVGGGLLRAGETAGVDLHNPEVTVMLEVRDSDLYIIEQRYEGLGGYPIGAQDDVLSLMSGGFDSTVASYLTMKRGLKTHFCFFNLGGAAHEIGVKQVALYLWEKYGVSHRVKFVAVPFEEVVAEILKNVHHSQMGVILKRMMLRAAEKVADQMGIEALVMGDSVAQVSSQTLANMSVINKVTDTLVLRPLITMDKQDIISLSAKIGTEEFAKNMPEYCGVISDRPTTRAKMERILEEEDNFDFEVLERAVEQRVSTKIDEVMSRIQTIAEVDLVNVPEVDDVIIDIRHPDEVEKSPLVLTNNEIKNIAFYELMSKLDQLEADQQYLLYCEKGTMSQLHASHLKGMGYEKIGVYAP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein contains novel Rhodanese domains that confer a unique function or activity.2. A protein that can perform ATP binding, tRNA U4 sulfurtransferase in cytoplasm to support tRNA thio-modification.3. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor.4. The design of the protein must focus on the precise arrangement of amino acids in the Cysteine persulfide intermediate to enable effective catalysis of [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 +   S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier   protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-   cysteinyl-[cysteine desulfurase].5. The protein design should take into account the physiological conditions of the Cofactor biosynthesis pathway. The designed protein sequence is ",
    "response#1": "LGTIDAAVSLISGGFDSTVSSYLTMKRGMRTHFCFFNLGGREHEVGVKEVALYLWLKYGASHRVKFVSVPFEDVVGEILKNVENSQMGVILKRMMLRAASEVAAQLEVSALVTGEAVAQVSSQTLQNLSVIDQVTDTLVLRPLICADKLDIINIAAKIGTEAFAANMPEYCGVISVKPTTRDNITVEEDKISYSECIRIKFENQEDIVKKVHALYEEKVKGKTFGVRCRRTGTHSFTSIDMEKAIGDALYSISNGVDLKSPEVWIHVDIVGKDALIYDKYSRQMIVPNFGLQGQLRLRAAKVLLVGAGGLGCPAAAYLAGAGVGVLGLIDGDTVEVSNLHRQVAHSTGRVGMTKVESAITYLKELNPSITYNAHQTHLTPQNAASVLSQYDLVLDCTDHPASRYLISDACVLLGKPLVSASAFQASGQLLVLNDPPGGGPCYRCVFPRPPPPESVVGCGEGGIVGPVVGTMGLLQALEAIKLIIRIKFFAEITIKTTPVRKRFVKQLKVNLRGLLAEIDPALRVHGSWDSLQIEGVGADNLAATLDCLQRTPGISQIQEVHEYPLTDLPALADLCVQHLGAQLAGKVFAVRCKRIGQHAFSSPEVASYLGRELSERCGAAGISLSAPEVRAEVEIRHDRVLLIQARHAGMGGFPLGTMEPVLSLMSGGFDSTVASYQLLQRGLLPHFIFFNMGGRAHEIGVRQIAHHLWRQYAGSHRLRFISVPFEEVVGELIRNVDDSQMGVVLKRMMLRAADRVAHQLGYQALVTGEAIAQVASQTLPNLAVIDRAVERLVLRPLITTSKQAIITQARHIGVETMCSSIPEYCGVISVSPAIYTTHTRVAEVEAQFDFNVL",
    "response#2": "LVKLFPEIIIKSKPVRKKFTKQLRDNLRKLLLPLDPNLEIIRDWDRITIETASRDPEVLAGFVAVLSNTPGIAHIVEVLEYPLVDVHDIFEKTRLAIGDSLRGKSFVVRCKRVGSHDFNSSEVERYVGGGLNQHIESAGVDLHNPDVTVRIEVRQDLVYIVNRRIEGLGGFPLGTIDAAVSLISGGFDSTVSSYLTMKRGMRTHFCFFNLGGREHEVGVKEVALYLWLKYGASHRVKFVSVPFEDVVGEILKNVENSQMGVILKRMMLRAASEVAAQLEVSALVTGEAVAQVSSQTLQNLSVIDQVTDTLVLRPLICADKLDIINIAAKIGTEAFAANMPEYCGVISVKPTTRAKPEKIAAQEANFDYSRQMIVPNFGLQGQLRLREAKVLLVGAGGLGCPAAAYLAGAGVGVLGLVDGDAVEVSNLHRQVAHATSRVGMPKVESAITYLKELNPTITYRAHPTHLTPQNAASIISQYDLVLDCTDHPTSRYLISDACVLLRKPLVSASAFQASGQLLRLNHPVARGPCYRCVFPRPPPPESVVGCGEGGVVGPVVGAVGVLQALDAIKLITQGGLEEAAADAPQQQASMLIFSAMADTMFRTVRLRGRREDYGRQLIIPTVGIKGQLRLKASSVLIIGAGGLGCPAAAYIAGAGVGTLGLVDGDTVETSNLHRQIAHATSRVGMSKVESAIAYLRDLNPRVNYRAHTTHLTPQNAEEIVSRYDLVLDCTDHPTSRYLISDICVLLGKPLVSASAFKTDGQLIVLNNPPAPQGSSPENGGPCYRCVFPRPPPPDSVVSCGEGGILGPVVGVMGILQALEAIKLVAAGAVDPTVEKPAAAPPSLLLFSGTSVPPFRSVRMRGRRKD",
    "response#3": "LVKLFPEIIIKSKPVRKKFTKQLRDNLRKLLLPLDPNLEIIRDWDRITIETASRDPEVLAGFVAVLSNTPGIAHIVEVLEYPLVDVHDIFEKTRLAIGDSLRGKSFVVRCKRVGSHDFNSSEVERYVGGGLNQHIESAGVDLHNPDVTVRIEVRQDLVYIVNRRIEGLGGFPLGTIDAAVSLISGGFDSTVSSYLTMKRGMRTHFCFFNLGGREHEVGVKEVALYLWLKYGASHRVKFVSVPFEDVVGEILKNVENSQMGVILKRMMLRAASEVAAQLEVSALVTGEAVAQVSSQTLQNLSVIDQVTDTLVLRPLICADKLDIINIAAKIGTEAFAANMPEYCGVISVKPTTRAKPEKIAAQEANFDLGTQEDVLSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGSAHEIGVKQVAHYLWSKYGSSAKVRFISVDFEPVVAEILEKVDDGQMGVILKRMFMRAAGIIAEKFAIQGLVTGEAIGQVSSQTLTNLRHIDGVTDRLILRPLINWDKEDIINLAREIGTEDFAKTMPEYCGVISKKPTGSEGKLISMVSGGIDSPVATWLMMKRGSPCDIFFCSLADPIDTQAFLEIAKKLVERWSPYRDGNVFIADCRDLIRDMVIEKKTNFNNVTFKKVLYRLAERLAEKYRYLGIVTGESLGQVSSQTAENLLSIEHGINFPIYRPLIGLDKDEITAIARDIGTFPEKNVGEFCSLFSAHPVTRSKWEDIEEDVKKIPKLIAALQRIPGIANILKVESHELEGIDDRFEDIADICIKTYGDQVSGKSFVVRVKRAGQHSFASTDLERYLGGAFLQNTKASRVELREPDVAIKLEIRDQTLYAIAE"
  },
  {
    "reference": "MSKRKEFDEDAPVDPSDLLEFINLGAGNEVGRSCHVIQYKGKTVMLDAGVHPAYTGLSALPFFDEFDLSTVDVLLISHFHLDHVASLPYVMQKTNFRGRVFMTHPTKAVCKWLLSDYVKVSNVGMEDQLYDEKDLLAAFDRIEAVDYHSTIEVEGIKFTPYHAGHVLGACMYFVEMAGVNILFTGDYSREEDRHLHVAEVPPKRPDVLITESTYGTASHQPRLEKEARLLNIIHSTIRNGGRVLMPVFALGRAQELLLILDEYWNNHLDLRSVPIYYASSLARKCMAIFQTYVNMMNDNIRKIFAERNPFIFRFVKSLRNLEKFDDIGPSVILASPGMLQNGVSRTLLERWAPDPRNTLLLTGYSVEGTMAKQITNEPIEIVSLSGQKIPRRMAVEELSFAAHVDYLQNSEFIDLVNADHIILVHGEQTNMGRLKSALASKFHNRKVDVKVYTPRNCVPLYLPFKGERLVRALGKVAVHKPKEGDIMSGILIQKDANYKLMSAEDLRDFSDLTTTVLTQKQVIPFFSSMELANFHLKQMFGYVKQSKTKAGQPQYTVMDAITLTLIQEHKLALEWVGNIMNDTIADSVITILLGIESSPASVKLTSHKCNHLHSHLDKPPKVSKEEDRIKKLMMFLDNQFGESMTKTEKGVEIKFEKYEASIDFSTMKVECSNEALRSRVVHVLSRAINTILPFSEASQNDVSEDDFENEESDDDKIFEQQTKIEDDVKNENKTEPVEEQKSEEKNEQPNLKKEELS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be optimized for mRNA cleavage and polyadenylation specificity factor complex, cytosol-specific folding and stability, while maintaining endoribonuclease activity, 5'-3' exonuclease activity.2. Prioritize Basic and acidic residues, Acidic residues compositional bias within the protein sequence to achieve desired characteristics.3. The protein must exhibit the following characteristics: Component of the cleavage factor I (CF I) involved in pre- mRNA 3'-end processing..4. The Zn(2+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "SGLETHIENSYANAYLQALHHLIPFRKIAESHAMSSAPLDPSKGGCQKDDCLLCQFGFLSKMLEDAKGANCQATNFLRSLSGSSRAASMGLMDKDDASNADAAYSSLIQTFNRFLLETVSVESAEAASPSSNDVDLMRRMFVNTQYTVQVCGTCGTVSQRPSGSNIVDLIYPRKHLSNELAPPSDFASILRTSLLRETLAKATCRTCNTPASAIRSQRVLPPIESLPQALSVNCVINTSDQLRYCPHCQGRGLVRSVESSGLAVLRAIEEEGGRRRAAEIVVHCATPVAFWLLNKKRERLMEDEQRFGMSILVQADDSLAGPGVRIERETTRRLHLLTQHELNGTITGLAATRTLESTIDGLDRLIVSFKDAKMALLEWSRGDIATVSLHTYERCSQMNTGDLQSYVPLLRTDPLSRLAVLTLPEDSLAVLPLIQEQSELDPLSEGFSRDAPYSPSFVLSLSDMSITIKNIQDLLFLPGFHSPTIALLFSPMHTWSGRLQTVKDTFCLEIRTFDLSSGTSYPLLTSVSGLPSDSLYLVACPSELGGIVLVTSTGIVHVDQGGRVTAACVNAWWSRITSLKCSMASVSQKLTLEGSRCVFVTPHDMLLVLQNGAVHQVRFSMEGRAVGVIEVLDKGCVVPPPSDLTVAGDGAVFVGSAEGDSWLAKVNVVE",
    "response#2": "GLENNITNSYANSLLQVMHYTPLLRNMALQHAATACLADPCLLCELGYVFDMLQKGEGPSCHATNMLRALNHTSNASVSGVLEDIAKDKNPSTLVKNLTMFLFDKISQDYKGTPPISTELERTLFKLNQPPNPLDLVKRLLETDARYQIKCMHCQHVSPRTATTFVNKLCYPAAKPNIRGMKAQRITFSQVLKAGLENEAVNKGYCTKCQRYQNLDQRKIIFNIPAVLALCTEITTAEHRKLWSTPGWLPEEIGIIVDQGHR",
    "response#3": "GLENNITNSYANSLLQVMHYTPLLRNMALQHAATACLADPCLLCELGYVFDMLQKGEGPSCHATNMLRALNHTSNASVSGVLEDIAKDKNPSTLVKNLTMFLFDKISQDYKGTPPISTELERTLFKLNQPPNPLDLVKRLLETDARYQIKCMHCQHVSPRTATTFVNKLCYPAAKPNIRGMKAQRITFSQVLKAGLENEAVNKGYCTKCQRYQNLDQRKIIFNIPAVLALCTEITTAEHRKLWSTPGWLPEEIGIIVDQGHLS"
  },
  {
    "reference": "MDPFIGSTPRETVKQVSDLLGCSPTSAEVASYFDQHDTLRHLREKFLVPKIADLPPSDPSLVDGSKECIYLVGNSLGLQPKMARKYLEEELDKWAKTGVHGHTEGSRPWAWAENNIEELMANVVGAKTEEVALMNGLTVNLHLLMLSFYKPTAARHRILLEDKAFPSDHYAVESQIRLRGFDPKQSMLLLSPRPGEETLRTEDILDVIEKEGDSIAVVMFSGVQYYTGQLFDMAAITEAGQRKGCYVGFDCAHAAGNAELKLHDWGVDFACWCSYKYLNSGAGGIAGAFIHKKHKDTIKPALLGWWGHDLKTRFQMDNKLHLQSGVSGFRLSNQPILLVCPLQASLEVFNMTSMQALRRKSLLLTGYLEYLIRHYYTEDPAKPHKPHIHIITPSDPRQRGCQLSLSFSVPIRKVFQELEKRGVCCDMREPSVLRIAPVPLYNTFSDVHRFVKTLGSALGSSSAGCCKF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a high affinity for pyridoxal 5'-phosphate.2. A protein that contributes to anthranilate metabolic process, tryptophan catabolic process, quinolinate biosynthetic process, L-kynurenine catabolic process, 'de novo' NAD biosynthetic process from tryptophan by functioning in cytoplasm with kynureninase activity, pyridoxal phosphate binding, 3-hydroxykynureninase activity.3. The designed protein should contain one or more Aminotransferase class V domains that are essential for its function.4. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis, NAD(+) biosynthesis pathway to avoid interference with native metabolic processes.5. For general function, the protein need meet that Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively.6. The desired protein should be able to perform H2O + L-kynurenine = anthranilate + H(+) + L-alanine The designed protein sequence is ",
    "response#1": "MIYLCGNSLGLQPKRTVTRLQQYLSTWATQGVQGHFKPLDDSPLPTWLDADARAAELIAPVVGADKSEVAVMQTLTANLHFLMSAFYRPDINGRHKIILESKAFPSDHFAVETQLRHHGLSPETSMITLKSPHSEDNVLTTDDITSAIAEHADSTALILLPGIQYYTGQLLDIRTITAFAHKHGIFIIWDLAHAVGNVPLALHDWDVDAAAWCTYKYLNGGPGCIQPKMAKKYIRRRNNIEGLMANVVGAKAEEVALMNGLTVNLHILLLSFYKPTAKRHKILLEDKAFPSDHYAVESQIQLKGYDPQQSMLLMKPRPGEEVLRTEDILDTIEKEGDSIAVVLFSGVQYYTGQLFDMATITNAGQNKGCYVGLDCAHAVGNAELKLHDWGVDFACWCSYKYVNSGAGGLAGAFIHEKNARTVKPALTLWGAQPKMAKKYIRRRNNIEGLMANVVGAKAEEVALMNGLTVNLHILLLSFYKPTAKRHKILLEDKAFPSDHYAVESQIQLKGYDPQQSMLLMKPRPGEEVLRTEDILDTIEKEGDSIAVVLFSGVQYYTGQLFDMATITNAGQNKGCYVGLDCAHAVGNAELKLHDWGVDFACWCSYKYVNSGAGGLAGAFIHEKNARTVKPALTV",
    "response#2": "MIEQPKMAKKYIRRRNNIEGLMANVVGAKAEEVALMNGLTVNLHILLLSFYKPTAKRHKILLEDKAFPSDHYAVESQIQLKGYDPQQSMLLMKPRPGEEVLRTEDILDTIEKEGDSIAVVLFSGVQYYTGQLFDMATITNAGQNKGCYVGLDCAHAVGNAELKLHDWGVDFACWCSYKYVNSGAGGLAGAFIHEKNARTVKPALTG",
    "response#3": "MKIYLCGNSLGLQPKRTVTRLQQYLSTWATQGVQGHFKPLDDSPLPTWLDADARAAELIAPVVGADKSEVAVMQTLTANLHFLMSAFYRPDINGRHKIILESKAFPSDHFAVETQLRHHGLSPETSMITLKSPHSEDNVLTTDDITSAIAEHADSTALILLPGIQYYTGQLLDIRTITAFAHKHGIFIIWDLAHAVGNVPLALHDWDVDAAAWCTYKYLNGGPGCIQPKMAKKYIRRRNNIEGLMANVVGAKAEEVALMNGLTVNLHILLLSFYKPTAKRHKILLEDKAFPSDHYAVESQIQLKGYDPQQSMLLMKPRPGEEVLRTEDILDTIEKEGDSIAVVLFSGVQYYTGQLFDMATITNAGQNKGCYVGLDCAHAVGNAELKLHDWGVDFACWCSYKYVNSGAGGLAGAFIHEKNARTVKPALTL"
  },
  {
    "reference": "MILLENGVRVFPKPGMGKDIYIGLANFGFENDVPELLGVAHLLEHILISFDYTRFVANASTARTYMSFWCRALRAEDYLAALETAVSWFFARGALRTDFSRVRIRNYVRELENEYYFRNEVFHCMDILTFLGGGDLYNGGRLSMLEQLDAVRELLGKRMRRLAGPNVVIFVRELSPAALALLERSFGTLPRFPSTIPATRLGSIHNKAVLVPAPFYALLIQVDNTVENVLAVICLAESYHFVDYETLGERLYVSFAFVHEQDCEAFLRNVGELRFEPAPRVELNYSDDYVMNLYVNFPWLQHDLADYLYTLNADCVPLLRGLEENLRRSVRERQLVVVYPSFSPSLFNSRDRQDHRLLVLDVDLARSAGPARVPRTFRRQPRAEVFVRYGDPALLDYVAFALARPRAAALRRLPRGVRLAHGFSHADMHEIMASETFIKYSRSRPAALFQYIFLAFFATGRSIAEILERREALVSFDARRCVNRLVFAKRARYDVVTKSSFVCGVLRGPRLSEAALTRAMWELKRKGLLYSLEHTRMHAKHTFYVFAFSIYPEQVYRYFARWQLVSKHCCVVSMRGEREDYSALRKEVVVNFV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in proteolysis, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "QLTIPIRVDQNGAFLSFTVKNDKHSRRRRSMDPIDPQQAVSKLFFKLSAYGKHFHLNLTLNTDFVSKHFTVEYWGKDGPQWKHDFLDNCHYTGYLI",
    "response#2": "QLTIPIRVDQNGAFLSFTVKNDKHSRRRRSMDPIDPQQAVSKLFFKLSAYGKHFHLNLTLNTDFVSKHFTVEYWGKDGPQWKHDFLDNCHYTGYLG",
    "response#3": "QLTIPIRVDQNGAFLSFTVKNDKHSRRRRSMDPIDPQQAVSKLFFKLSAYGKHFHLNLTLNTDFVSKHFTVEYWGKDGPQWKHDFLDNCHYTGYLG"
  },
  {
    "reference": "MLTTLLQSDLPGLPLRHCGKVRDVFDIPRKRLPVDTRSGEYLLIVATDRLSAFDVVLPDPIPGKGEILCQISNFWFQKTEHLMPNHLTGINVASVLPDGIDKTLYIQRAVVTKKLKPVGIEAIARGYLIGSGWKDYQRTGKVSGIQLPDGLQEAEKLPDPIFTPSTKAAVGHHDENIDFDTTVKMVGAELAERVRDATLRIYHFAAKYAAECGILLADTKLEFGTDIDGRLYVMDEMLTPDSSRYWPIDEYQVGTSPPSYDKQLVRNYLETLDWDKTAPGPTLPQDIIDRTRAKYTEALQRLAGINID",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must be optimized for phosphoribosylaminoimidazolesuccinocarboxamide synthase activity, ATP binding.2. The protein should be optimized for activity in IMP biosynthesis via de novo pathway, 5- amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5- phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2 pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. We need a protein that can perform 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP +   L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-   4-carboxamido]succinate + ADP + 2 H(+) + phosphate with high efficiency. The designed protein sequence is ",
    "response#1": "LYTGKAKSVYRTDDPEVYIMKFRDDITAFDGEKKDTLEGKGRYNAEVSTFIFRYLEEHGIRTHYLASIESGVIAVRNLTMIPLEVIVRNVAAGSIVRNYPFREGEPLDPPLIVIDYKSDAHHDPMLNDELIYALGLATPEELDQIKAMALAINELLSDYLDLRGITLVDFKMEFGRYNGEIVVGDEISMDSMRLWDKETGTSLDKDVYRFGKGDVMETYAGVAKLYTGKAKSVYRTDDPEVYIMKFRDDITAFDGEKKDTLEGKGRYNAEVSTFIFRYLEEHGIRTHYLASIESGVIAVRNLTMIPLEVIVRNVAAGSIVRNYPFREGEPLDPPLIVIDYKSDAHHDPMLNDELIYALGLATPEELDQIKAMALAINELLSDYLDLRGITLVDFKMEFGRYNGEIVVGDEISMDSMRLWDKETGTSLDKDVYRFGKGDVMETYAGVAKLYTGKAKSVYRTDDPEVYIMKFRDDITAFDGEKKDTLEGKGRYNAEVSTFIFRYLEEHGIRTHYLASIESGVIAVRNLTMIPLEVIVRNVAAGSIVRNYPFREGEPLDPPLIVIDYKSDAHHDPMLNDELIYALGLATPEELDQIKAMALAINELLSDYLDLRGITLVDFKMEFGRYNGEIVVGDEISMDSMRLWDKETGTSLDKDVYRFGKGDVMETYAGVAKQIYEGKAKILYEGPEPGTLIQYFKDDATAFNAQKRGTINGKGVINNRISEYVFTRLAHIGIPTHFIRRLNMREQLVRQAEIIPIEVVVRNIAAGSISKRLGIEEGEPLPHTLIEYYYKDDALGDPMIAEEHIACFGWASNEEMQDISSMAIRVNDFLCGMFAAINISLVDFKLEFGRIWDGDYSRVILADEISPDGCRLWDMTTGEKLDKDRFRRDLGGEEEAYQEVAR",
    "response#2": "KIYEGKAKILYEGPEPGTLIQYFKDDTTAFDATKKAVLEGKGVINNRISEYIMTKLNSIGVQNHFIKRLNLREQLIREVEIIPLEVVCRNVAAGSLSKRFGLPEGQALPRSIIEFYLKDDKLHDPMVAEEHITAFNWASTQEIDDMMALTLRVNDFLTGLFSGVGITLVDFKIEFGRIWENDFSRIILADEISPDSCRLWDSQTNEKLDKDRFRRDLGNVIESYTEVARLYTGKAKSVYRTDDPEVYIMKFRDDITAFDGEKKDTLEGKGRYNAEVSTFIFRYLEEHGIRTHYLASIESGVIAVRNLTMIPLEVIVRNVAAGSIVRNYPFREGEPLDPPLIVIDYKSDAHHDPMLNDELIYALGLATPEELDQIKAMALAINELLSDYLDLRGITLVDFKMEFGRYNGEIVVGDEISMDSMRLWDKETGTSLDKDVYRFGKGDVMETYAGVAKRELIYEGKAKKLFSTDDENLLISEFKDDLTAFNGEKKSSEVGKGALNNKISTELFKLLEKSGIQTHFVKMLDDNHLLHKKTKVILIEVIVRNIATGSLSKNLGIKDGTVLPFTLVEFDYKNDALGDPKLNDQHALILGLVEYQDELDKLRRMARQINDILKPYFAQKGLNLVDFKLEFGKDSSGNIILIDEISPDNCRFWDIESGEKMDKDRFRQGLGGLRVAYEQVLSRILENI",
    "response#3": "IYEGKAKKLFSTDDENLLISEFKDDLTAFNGEKKSSEVGKGALNNKISTELFKLLEKSGIQTHFVKMLDDNHLLHKKTKVILIEVIVRNIATGSLSKNLGIKDGTVLPFTLVEFDYKNDALGDPKLNDQHALILGLVEYQDELDKLRRMARQINDILKPYFAQKGLNLVDFKLEFGKDSSGNIILIDEISPDNCRFWDIESGEKMDKDRFRQGLGGLRVAYEQVLSQGKVRDVYDLGDRLLIVSSDRISAFDYILPTGIPDKGRLLTAMSRFWFEQMDAGRIGQNVGSAGGDGSSNPRSISHHLISTDVPEDVAAVVDPKPLEGRVMVTRKASVVPFECVVRGYLEGSGWKEYQATGEVCGVSLPAGLKQCDQLSEAIFTPATKAEEGHDENVSYEVMSQSLGEEQSSQLRRMSLAIYQDASKIAAERGLLIADTKFEFGVVDGELMLIDEVLTPDSSRFWAADEYEPGHSQRSFDKQFVREYLQESDWDRNRELIYEGKAKKLFSTDDENLLISEFKDDLTAFNGEKKSSEVGKGALNNKISTELFKLLEKSGIQTHFVKMLDDNHLLHKKTKVILIEVIVRNIATGSLSKNLGIKDGTVLPFTLVEFDYKNDALGDPKLNDQHALILGLVEYQDELDKLRRMARQINDILKPYFAQKGLNLVDFKLEFGKDSSGNIILIDEISPDNCRFWDIESGEKMDKDRFRQGLGGLRVAYEQVLSRILG"
  },
  {
    "reference": "MKLLTTPRFDQAKVLVIGDIMLDRYWHGATSRISPEAPVPVVKVGQIEDRPGGAGNVALNIASLGGAAFLAGPTGNDESADILTNRLTAAGVHCAFTRVSDEPTITKLRVISQHQQLIRLDFEEAFNSLEPGQLVQEVEAIIDQAEVVVLSDYKKGALKNPQQLIQLAKTKGKAILVDPKGSDFSQYRGATLITPNLNEFEAVMGICKDEAELVAKGQQLLKALELEALLITRGEHGMTLLRPDQPELHLPARAREVFDVTGAGDTVISVLAASLAAGEKLPTAVALANLAAGIVVGKLGTAAISAPELRRAVSAEQGAGRGVMTASQLLIAIEDAKAQGEKIVFTNGCFDILHAGHVGYLEQARAHGDRLIVAVNSDESVHRLKGEGRPVNPVDRRMTVLAGLEAVDWVVSFAEDTPEALLALVKPDVLVKGGDYSVKQVVGAPIVESYGGEVAVLDFLDNCSTTAIVEKIKEQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7- phosphate at the C-1 position to selectively form D-glycero-beta-D- manno-heptose-1,7-bisphosphate.. The designed protein sequence is ",
    "response#1": "MTNGCFDILHAGHVSYMNHAAELGDRLIVAVNTDESVKRLKGPSRPINPTDRRMAVLAGLGAVDWVVPFSEDTPQRLISEVLPSILVKGGDYKPEDIAGGEEVIAAGGEVKVLNFEDGCSTTEIIA",
    "response#2": "MTNGCFDVLHAGHITYLEEARSLGNRLIVAINDDNSVKQLKGNNRPINTLQDRMILISALRCVDWVVSFSEDTPERLITRVLPDILVKGGDYSIPDIAGSKQVLEG",
    "response#3": "MTNGCFDILHAGHVSYMNHAAELGDRLIVAVNTDESVKRLKGPSRPINPTDRRMAVLAGLGAVDWVVPFSEDTPQRLISEVLPSILVKGGDYKPEDIAGGEEVIAAGGEVKVLNFEDGCSTTEIIA"
  },
  {
    "reference": "MPITPRNIVRHELIGLECRVVKSLGPPYEGLEGRIVDETKNTLVLKTESGEKVIVKDQVLLELKLPSGERVRVDGALLVGRPEERLSKRIKYAEVVRGRFDPEDYLD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.. The designed protein sequence is ",
    "response#1": "MEWSYIILEVVGGTTPIDEIDDISLRHLITLALNQSFGIFGSAIPIDFLHRQSKFLYLRCHKEDKKKVLVALGSS",
    "response#2": "EWSYIILEVVGGTTPIDEIDDISLRHLITLALNQSFGIFGSAIPIDFLHRQSKFLYLRCHKEDKKKVLVALGSS",
    "response#3": "EWSYIILEVVGGTTPIDEIDDISLRHLITLALNQSFGIFGSAIPIDFLHRQSKFLYLRCHKEDKKKVLVALGSS"
  },
  {
    "reference": "MLKGKNILLGVTGSIAAYKIANLASMLVKLGADVHVIMTKNATQFITPVTFETLTKNRCYTDTFERTYPVEVSHIALAQRADAVLVAPASANILAKMTYGIADDMLTSTLLAVTAPVMVAPAMNVHMFESAPVQENLAALQKRGVTVIPPDEGYLACGDTGKGKLPSEQTLCDYLLRELAREKDLAGKKVLITAGPTRESIDPVRFITNHSTGKMGYAVARQAMLRGAEVTLISGPVNIAPPPFVNVVRVQSAQEMFEAVKANYEDMDFIIKTAAVADYTPADFHGEKIKKSDDGSDLSLPLKRTQDILKWLGEHKKDGQKLCGFSMETENMLENSRAKLARKNADLIAANSLRDAGSGFGTDTNHLVLISKDSVDDLPLLSKEDAADRVLDALQKQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..2. The protein contains novel DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains that confer a unique function or activity.3. The protein design must be able to perform phosphopantothenoylcysteine decarboxylase activity, phosphopantothenate--cysteine ligase activity, metal ion binding, FMN binding in order to contribute to coenzyme A biosynthetic process, pantothenate catabolic process.4. The designed protein should interact with enzymes and substrates within the coenzyme A biosynthesis, Cofactor biosynthesis pathway to enhance or regulate its activity.5. The protein requires Mg(2+) to activate a critical catalytic site.6. The CTP binding site should be located in a region of the protein that is accessible to the ligand.7. A key design requirement for the protein is the optimization of the Proton donor to achieve high catalytic efficiency for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2. The designed protein sequence is ",
    "response#1": "KKLLLGVTGGVAAYKVAELARLLTQKGVVVQTVMTKSACRFVGPATFQSLTGLPVFTELWEANVASNMAHINLSRNVEMILIAPASADFIAKLACGLADDLLSTLCLARNCPMMIAPAMNRQMWENPATQRNINRLQDDGVIVAGPADGEQACGEVGPGRMLETDAIAAAVRTLFKGKGLQGKSVMITAGGTIERIDPVRYITNDSSGKMGFAIAEAARDYGAKVTLIAGQTQVEPPEGVEVIRVESTQDMYDAVMSRFDNMDIIVKAAAVSDYRPAVRADQKIKKTTDRMTIELVKNPDILQELGARKNGQFLIGFAAETHDVAAFAQDKLRRKQCDLLVANDVSRDDAGFGSNNNEVQVFFPDGSVELQGKKLLITAGPTRENIDPVRFIMNRSTGKMGYAIAEQAEKMGAQVTLVSGPVSLAPPANVNVIDVESAAEMHKEVLKHFTDADAVIKTAAVADYTPKFHHEHKMKKQPGESSIPLVGTADILKELGSKKDKQILVGFAAETENVEQYAIGKLQKKNIDMIVANNVAAEGAGFGTDTNIVTLILFADGTREELPKMEKSVLAERIVDAVSRKL",
    "response#2": "KHLVLGMTGGIACYKIAELTRLLTKAGATVQVAMTEAATQFITPVTMQALSGRPVFTSQWDTRIDNNMAHIDLSREADAIVIAPASADFIAKLVHGMANDLLSTLCLARDCPLLVVPAMNRQMWANPATQRNVAQLRADGILVLGPDSGSQACGEIGDGRMLEPEATYEAVIRTARRVLGDLSGKRVLISAGGTREFLDPVRFIGNPSTGKMGIALAQAALDRGADVTLVHAPLGILEPLPGNLEAIAVVSAAEMHEAMLQALPQADWIIMAAAVADVKPSHTSLEKLPKSALPDSLPLVPVPDIVTELARHKQPHQRLIGFAAQTGDIVTPAKDKLERKKLDAIAANAIDQPNSGFGSDMNQAVLTVATSARRQDPLEGKTVIVTAGGTQEAIDPVRYIGNRSSGKMGYAVAEQAALAGARTILISAPSALPVPYGVEFVPVDSARDMEEAVNSRYEDCDAVIMAAAVADFRVENMADTKIKKMDTLELKLVKNPDILKGLGANKTHQKLIGFAAETQNVIEYGKSKVLNKNLDMLVANDVSKQNAGFNVDTNEVTFLMGADGTDRKVEGAKHDVARKLLDLAVAAIAAP",
    "response#3": "KKILLCVTGGIAVYKAAALTSKLTQAGADVKVIMSASACEFVTPLTFQALSRHDVYTDTFDEKNSEVIAHIDLADWADLVLVAPATANVIGKLANGIADDMITTTMLAVHAPVWVAPAMNVNMYAHPAVRKNIETLRSYGYQFIEPSEGYLACGYVGKGRLEEPEAIVEQLLRWFGLPKPLSGKRVLISAGGTREFLDPVRFIGNPSTGKMGIALAQAALDRGADVTLVHAPLGILEPLPGNLEAIAVVSAAEMHEAMLQALPQADWIIMAAAVADVKPSHTSLEKLPKSALPDSLPLVPVPDIVTELARHKQPHQRLIGFAAQTGDIVTPAKDKLERKKLDAIAANAIDQPNSGFGSDMNQAVLDVLGPPPTALAGRRVLITAGPTFEPIDPVRGITNRSSGKMGFAIARAAQQAGAAVRLVAGPVALATPWGVVRDDVETARQMYDAVLSTAADCDVFFSVAAVADWRVDEASVDKIKKTAGHRVPSLAFVENPDILATVAQLPNAPYCVGFAAESGNLETHGEEKRVRKNVPLLVGNLGPKTFGRDDNEVVVLWPGGDARIALGPKARLAHEIWDAVLRLR"
  },
  {
    "reference": "MSMGLEITGVSLAVLGWLGTIVCCALPMWRVTAFIGTSIVTAQIIWEGLWMNCVVQSTGQMQCKVYDSLLALPQDLQAARALLVVAILLAAFGLLVSLVGAQCTNCVQDDTAKAKITIVAGVVFLLAGVLTLIPVSWSANTIIRDFYNPLVAEPQKREMGAGLYVGWAASALQLLGGALLCCSCPPKEKRYTPAKILYSAPRSTATTAAGLGPSYDRKDYV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The designed protein should have structural molecule activity within bicellular tight junction, apicolateral plasma membrane, lateral plasma membrane to regulate regulation of cell morphogenesis, positive regulation of metallopeptidase activity, positive regulation of gene expression. The designed protein sequence is ",
    "response#1": "SLRYLYLSGNKITKLTAYQFQTFEQLEMLDLTNNEIGAIPANSLSGLKQLRQLYLAHNKE",
    "response#2": "HHLVFHVANICFAAALVIPTTLTLHMIFLRGLLTIGCALFIVWATLYRCALDIMIWNSAFLLINLLHFLYLLYKKRPIKIEKELTGVYRRMFEPLRVPPELFRRLTGQFCNIQTLKKGQTYAAEDKTSVDDRLSILLKGKMKVSYRGHFLHNIYPCAFIDSPEFRSTQMNRGEKFQVTIVADDTCRFLCWSRERLTFFLESEPFLYEIFRYLIGKDITNKLYSLNC",
    "response#3": "LTILMGIPNFLSAFFVFSQVFMVLDEAHYCSVSWVKNHTFNLSAAEQLAVSIPNDTAGRPESCLMFRPPPDNASLEDILSHRFNETQACDSGWDYPENRPPSLKNEFDLVCERENLKRTSQSVFMAGLLVGALVFGPVCDWIGRRPSLLMQLLLSGIASMATAFVSSFELYMTLRFALATANAGFLLSSNVLLSEWVGTSRRTRAMVLAQSNFALGLMALAGLAYGVRNWRLLQIIGTTPIFLLVFYFWVLPESPRWLLSQGRTEEAKQLVQKAALVNGRPLASELLNQLVPEKTGPSGNALDLFRHPQLRKVTLILIAVWFVDSLLYYGLGFQVGDFGLDIYLTQVIFGVVEVPARLSSIPMMEKLGRKWSQLCTLTLAGVMCVIIIFIPGDLPTVATALAVVGKFASSAAFTISYVYTAELLPTIIRQTGMGLVSIFSRVGGIITPLVMLLEQYHKAIPMLIFGSLPIGAGLLCALLPETRGKTLEEISGE"
  },
  {
    "reference": "MIIDFKKYSSVRIGNEFEVLVLDQICDFDGFLIGGANNLLISPKPKNIGILGDGFDFMQILDQNKDFIHLRIGCKTKSSKIYRFAKENNLKGFEYLSKIPGTLGGLLKMNAGLKGECISQNLIKIATSQGEILRENINFDYRFCPLNMPFFWAEFKLNFGFDTLKDEALKNARSNQPSGASFGSIFKNPKNDFAGRLIEAVGLKGFSKGDAMLSDKHANFLINKKNASFEDAFFLIELARKKVFEEFGINLENEVIII",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The presence of FAD is crucial for the protein to function optimally under specific conditions.2. For general function, the protein need meet that Cell wall formation.3. The design of the protein must focus on the precise arrangement of amino acids in the active site, Proton donor to enable effective catalysis of NADP(+) + UDP-N-acetyl-alpha-D-muramate = H(+) + NADPH + UDP-   N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine.4. The designed protein should have UDP-N-acetylmuramate dehydrogenase activity, flavin adenine dinucleotide binding within cytoplasm to regulate cell wall organization, regulation of cell shape, cell cycle, peptidoglycan biosynthetic process, cell division.5. A protein that can modulate the overall flux of metabolites through the Cell wall biogenesis, peptidoglycan biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MAAVRCRSQHSVVEVVRLLDAHQIPLLIVGGGSNLVIADGEIPLVAVILDCDDISVTLDTGRVVAEAGAVWDDVVRLCVDAGLGGIECLSGIPGSAGATPVQNVGAYGAEISDVLVSVTLLERATGAVMASMAFNEPSRVLAVAGTHGKTTTSSLVAQVLKECGLDPTVIVGGRLKAVGTGGVLGKGDYLVAEADESDGSFLKLSPTIAIVTNIDDDHLDYYKTFSNLEKAFADFLDRIPFYGVSLLCGDDPTLRKLMPRLKRRYETYGFGINCDLRASKVVTNAANTQFEVHYKAQKLGTLHLSLAGRHNILNALATVAMLVEAGFDAKSAAAGLGAVHGVPRRFEVRYKSENFMVIDDYAHHPTEVRATLAAARSGGWKRVVALFQPHRYTRTQSLQAEFAAAFADADLLFLTDIYAANEEPIEGVTGEAFAAAVEAGGHPKVQYEADLRKLRKIVAREIEPGDLVITMGAVGDATAWTQVMHAIIEIRQQKLPDPKQLPNCGSFFQNPIVPQDQFATLQSSYPAIVGYPMPDAMTKVAAGWLIEQAGLKGGGIEPIFTHQQQALVLTNHAPYIATKQDVAAAQKYIIDTVYKKFAIQLSREPVWDGAESVIAEAK",
    "response#2": "MAAVRCRSQHSVVEVVRLLDAHQIPLLIVGGGSNLVIADGEIPLVAVILDCDDISVTLDTGRVVAEAGAVWDDVVRLCVDAGLGGIECLSGIPGSAGATPVQNVGAYGAEISDVLVSVTLLERATGAVMGPATAAFALYGNPSRAMKMVGGPAQFWVEPQTEEGFIDLVRLCSTENIPLTVIGRGSNLLVRDGGIPGVVVHLGHGQFLQSQPNGHSIVAGAGVRFKQISCMAYRFGIGGFEWMEGIPGSLGGGLRMNAGAMEVQLFDQVVCVRCCDQKGNILIKTPPELNVHYRNTPDFACHYVLSATLQGYAGPVPLPTAVREAVLGLRAGKGMVYNEADHDTWSAGSFFTNPIVPESVGDHVRSVVGDESMPCFAAGEGMVKLSAAWLIDRAGFAKGHQGPGGRVSLSTKHTLALTNRGNATTDDLVALAREVRGGVMDAFGVLLEPEPVWIQSRWDESRGKRKSSQPVMASAGCTFKNPQEMPAGKLIDTLGLKGSHRGKAAVSDVHGNFIVNQGGATASDLLQLIDSIRSTARNERGIELETEVKILE",
    "response#3": "MAAVRCRSQHSVVEVVRLLDAHQIPLLIVGGGSNLVIADGEIPLVAVILDCDDISVTLDTGRVVAEAGAVWDDVVRLCVDAGLGGIECLSGIPGSAGATPVQNVGAYGAEISDVLVSVTLLERATGAVMAEDPRRAFSVLGTRVAVSRAICSIRSEKLPDPAVLGNAGSFFKNPLVSAAVVAQIKAQHPDLVAYAQPDGQMKLAAGWLIERAGWKGFREADAGVHKLQALVLVNYGAATGLQLLDLAQRIQKDIAERFNVELEMEPGPRSARVKEIKELRESKHPLDKPSLGSTFKNPEGDFAARLISECGLKGTIIGNAQIAEKHPNFVLNLGNATFKDIIDILTLVKKSVLEKFGIKLEEEIIII"
  },
  {
    "reference": "MAKFNKIRGSITAPLGFQAAARFCNVKKLGTGKGSNKGQKRDLALIVSDVPAKAAGMFTTNQICAAPVXLCVKHLRNNTAQAVVINSGNANACTGPQGLADAREMAALTXQALGLATTDVLVGSTGRIGVHLPMPNLRKGITAVAAQLKPTQAAATEAAEAILTSDTQTKQIAVEFRLGGRTVRLGGIAKGAGMIQPGMSPTGARPASAPXGLHATMLAYLTTDAKVDVANLKKCLREAVAXSFNCITVDGDMSTNDTVLLLANGLXGNGNLNANDVKIFQAALNHVCLELAKMIVHDGEGISRVVTVRVTGAKSFTEAXAAARAVGNSALVKTSWSGGDPNWGRIIDALGYSPAKVIEEKVDIAYSAXGSGKKVYSLRQGRPTGTAFGSLCKAVEPGEFDLHINLNLGEAAAMLHAADLTEEYVDFXKGDISDPASLGG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The substrate binding site should be located in a region of the protein that is accessible to the ligand.2. For general function, the protein need meet that Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.3. A protein that is capable of catalyzing reactions within the L-arginine biosynthesis, N(2)-acetyl- L-ornithine from L-glutamate: step 1/4, Amino-acid biosynthesis pathway with high efficiency and specificity.4. The designed protein is the optimization of the Nucleophile to achieve high catalytic efficiency for acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate.5. The ideal protein would be one that plays a critical role in arginine biosynthetic process through acetyl-CoA:L-glutamate N-acetyltransferase activity within cytoplasm. The designed protein sequence is ",
    "response#1": "MRRDRSWHDRAELDRLIADMGIAPPAGTGWXDVAXGXAVEXPXPXGDVXAXAXACXAVXXYPXARLVXXDGXSXDALDGLXAGXRSXGPVXAXGDXRXSXRXPLLRXGAPVXVXGXPXDVXXSXPXVLXVGPXGXXPXVXMVXRRXXGTXAXPXXPXRXLAXXAAPXDXLXGXGPXLPXXAXSWAXRXAPXSKIXAVXIAXXXRXTPXVVXXGXCA",
    "response#2": "MNNNNEFNQQDSNIMNMQQSNGMQQPNGMQQPNGMQQPNGMQQPNGMQQPNGMQQPNGMQQPNGMQQPNGMQQPNGMQQPNGMQQPNGMQQPNGMQQPNGMQQPNGMNWNQDFTNVSSYQNYNNDRPSYNNNVVPPYPGYTYDSYARRSYKKPPVKPKRRKKRNIWMLLLTLLLVIAVGGIAGGVVAAKMSKDSSSATTQADTSSATQTSDSTSSQASDTSSSSSNSSSDKTAAADVTKSQPTMIYEALKNV",
    "response#3": "MPTAQSTPALPAPTAATPIQPVPLTPEGKPA"
  },
  {
    "reference": "SVQFKPRL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Mediates visceral muscle contractile activity (myotropic activity).2. The designed protein must be able to regulate neuropeptide signaling pathway. The designed protein sequence is ",
    "response#1": "GHTARKRFGQNFL",
    "response#2": "MNILAPDL",
    "response#3": "MIS"
  },
  {
    "reference": "MINIKTNSRKVKPGDTFVAIKGFTVDGHDYIEDAIKAGATKIVCEHGSYSVETLVVPSTKEWVQKYIVDNYKDEVNKLRIIGVTGTNGKTTTCFLTYQALKKLGVNAAYMGTIGFYYGDTKYELNNTTPEILDLYSYFIEALEQGVTDVVMEVSSHSLCEKRVEGLEFSEVAFTNLTEDHLDYHKTMENYLNAKLLILKQIKKDGLVIVNNDDDYGKYFEVGNYKTLGYSGDNYKILDYSQTDKGTLISFMVDGKKYEIETNLRSKFNVYNYLTCLALLNNLGFSIDEIAEVTKSIYPPKGRCEQISVNGGEAVIDYAHTPDAVDKIISAFLENKKGKVITIVGCGGDRDPLKRPIMGNIASEKSDYVIFTSDNPRTEDPKAILEDIIKGVRKDNYEVEIDRPTAIRKGLDMIGKNDVLLILGKGHEDYQIIGHTKHHLDDAEEVRKYLEAHKN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be involved in peptidoglycan biosynthetic process, cell wall organization, cell cycle, regulation of cell shape by localizing to cytoplasm and exhibiting ATP binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity.2. The protein should be designed to catalyze the following reaction ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.3. The protein should be optimized for activity in Cell wall biogenesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. Incorporating a Meso-diaminopimelate recognition motif motif is critical for the successful design of the protein.5. The protein should have a high affinity for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate.6. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "MKLRKLLKGLDPVAVIGSTDIAVVSGLSVDSRQVAPGQVFFALAGAKTDGTKFIDQAIGKGAVAIVGEHAGEGFGDLLVPDMRRSYALMAARFYGNPSEKLKLIGVTGTNGKTTTTCLIHQIMNDHGIKAGMIGVTGTNGKTTIVDLLYNLYDSCGIKTGIISTIKIDVGDTTYNSTQTTPDSTLINKYLFEMVQNDVRYCFMEVSSHGIDQCRTHGLNFKGGVFTNLTHDHLDYHKNFKNYRNTKKIFFDNISSDGFAITNCDDKNGLYMIQNTNAETYTYSLKSNSSFKAKLLESSFEGMMIKINNSELWCNLVGEFNVYNLLAVYATCQALGIPLSEAIRATESLKPVPGRMEQVGTSAAGAPVLVDYAHTPDALETVLKAVRPHAHDRVVTVFGCGGDRDRTKRPAMGKIALREADHAIVTDDNPRSEDPAAIRAEILAAGPGLVEIGDRAAAIDAAIAGLGAGDVLVIAGKGHETYQIVGEEKFDFDDRKAAAEALDAREKNR",
    "response#2": "MKLERLLKDFPYKEIKGDLDIDISGLTADSRAVEPGNLFVALAGTKADGARYVGDAVARGAVAVVSCHPVEGVVVPVLTVSNPRRFLALAAARFYGDPSKKMKLIGVTGTNGKTTTTCLIHQIMNDHGCKAGLMGTIYYYVNGEKIDSNLTSPFAVELQKILYSMVKAGMDYCVMEVSSHAIELGRVYGCDFEVAVFTNLSQDHLDFHKTFEDYFYAKKRLFTDYLKKDGFAVINLDDKYADLMLRKTKAKVYTYAIESDADFIAENIKTTSSGTSFTLVTPSGNMEIKLKMVGKFNVYNALAAIAVASLLGMDLKTIKEGLEDIEAVPGRMEIFTGAGKPTAIVDYAHTPDALDKALSAAKEHCHGRLWVIFGCGGDRDNGKRSLMAKVAQSYTDNIIVTNDNPRTEDQDQIIEDILKGFSQGLRVEVIKERYQAIKWAMKQANEDDVILIAGKGHETYQILKNKTIYFDDREVAGEAIKELMGGR",
    "response#3": "MCVTGTAGKTSVAHFVRQIFEHCGHAAASLGTIGTITSNGKTYGGLTTPDPVTLHQDLARLAGDGITHAAMEASSHGLDQYRLDGVQLAAAGFTNLGRDHMDYHATMEDYLAAKMRLFTEVLPSGGTVVADPVEPYADRVIATCRARGLRVLTVGERGEDLRLTGLRHSGFSQVLTVATARGDYKVSLPLAGRFQVSNALIAAGLAQRHGVPADRIRVLGVTGTNGKTSTVQFAAQIWTRLGLPAGSLGTLGLLGPGLHGYGGMTTPDPVSLHRDLAAAKDAGIDHLAMEASSHGLEQFRLDGVAIKAAGFTNLTLDHLDYHGTMEAYRDAKAMLFDRVLPPGAVAVLNADSPEFQHFATICNHRGHRVLSYGLAGTELRVTGVEPLAHGQRLALSVLGRDVTVDLPLAGRFQAWNVLCALGLLEGLGVPLATALAGVASCPGVPGRLELAGVHASGAPVFVDYAHTPDALERVLAALRSHVERRLIVVFGCGGDRDPGKRPEMGRIAARLADRVIVTDDNPRSEEAATIRAAILAASPGAVEIGDRTAAIAAAVSDLSAGDVVVVAGKGHEQGQEFADVVVPFDDRQVARAVIADLVAG"
  },
  {
    "reference": "MNDNNNSNGGDKKNNRQPFYTLGILVLIALFFTSMMYKGASASTNQEISYTEFLDLVEDDKVSEVNFKDDVINITLKEGVSYSKSETTEEDEKLQQIYEAAGQPTAKVTLYTAYLKDDDLLDKLDEHGVKYEGTIADSTAAIIYNILSFVLPLVLLWVVLGFLMKRMGGGPMGVGKSNAKLYNMEKATGITFKDVAGQDEAKESVQEMVDFLHNPKKYTEIGAKLPKGALLVGPPGTGKTLLAKAVAGEAGVPFFSLAGSDFVEMFVGVGASRVRDLFKEAQKVAPCIVFIDEIDAIGKSRDAHYGGGNDEREQTLNQLLSEMDGFDSNKGLLILAATNRPEVLDKALLRPGRFDRRIIVDRPDQKGRLEILKVHAKDVKMDESVDLDALALASVGLVGSDLANIINEAAILAVKAKRQYVTQKDLFEAFELVAVGGKEKKDRAMSEKERKIVSYHEVGHALVSALQKDAEPVQKITIVPRTMGALGYTLQTPEEEKFLETKDELIAKIVTYMGGRAAEDIKFGSYTSGAANDIEQATKIARAMVTRFGMSDKFGMMGLATVESQYLDGRASLICGDNTAGQIDEEVLKMITDAYTKAKELLTENMESLDKISEYLFEHETITGKEFMKIFREIKGIPEPEEKKSENNSLLPEHKSIFEDDNDPNNVVTDSIFDE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have a high affinity for ATP.2. The designed protein must have at least one AAA+ ATPase domain.3. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.4. The designed protein should have ATP hydrolysis activity, zinc ion binding, ATP binding within plasma membrane to regulate protein catabolic process, proteolysis.5. The protein should have a Helical domain that is long enough to span the entire membrane.6. The protein design should emphasize Basic and acidic residues compositional bias.7. The protein must exhibit the following characteristics: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. The designed protein sequence is ",
    "response#1": "LLVGPPGTGKTLLAKAVAGEANVPFFSMSGSDFVEMFVGVGASRVRDLFRQAKEKAPCIIFIDEIDAVGRARGRNPNMGSNDERENTLNQLLTEMDGFGTNSGIIVLAATNRADILDKALLRAGRFDRQIYVAHLSFADSPEIERLEVEWLQRHNRQ",
    "response#2": "LLVGPPGTGKTLLAKAVAGEANVPFFSMSGSDFVEMFVGVGASRVRDLFRQAKEKAPCIIFIDEIDAVGRARGRNPNMGSNDERENTLNQLLTEMDGFGTNSGIIVLAATNRADILDKALLRAGRFDRQIYVI",
    "response#3": "LLVGPPGTGKTLLAKAVAGEANVPFFSMSGSDFVEMFVGVGASRVRDLFRQAKEKAPCIIFIDEIDAVGRARGRNPNMGSNDERENTLNQLLTEMDGFGTNSGIIVLAATNRADILDKALLRAGRFDRQIYVLSFHAPTKDLLIERNINLREVAERTPGFSGADLANLVNEAAILAAKKNKHQVYQEEVLKAIEIAIPGGA"
  },
  {
    "reference": "MSRTVHCVKLDHEAEGLDFPPYPGELGKRLYEQVSKEAWQMWMKHQTILINEYRLTLVDPKARQFLEQEMEKFFFGEGSTPPQEYTPPEQ",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Could be a mediator in iron transactions between iron acquisition and iron-requiring processes, such as synthesis and/or repair of Fe-S clusters in biosynthetic enzymes.2. The protein must be able to iron ion binding. The designed protein sequence is ",
    "response#1": "MK",
    "response#2": "GNFL",
    "response#3": "SFT"
  },
  {
    "reference": "MKIGVLTGGGDCPGLNAVIRAVVRKSLSLGHECLGIKEGWKGILENNYTPLTMESVSGILHRGGTILGTSRTNIYKVENGESKLKENMEKNGIDCIVAIGGEDTLGVASKLAASGVKVVGVPKTIDNDLNATDYTFGFDTAVNIATEAIDRLHTTAESHNRVIVVEVMGRHAGWIAVHSGIAGGADIILIPEKPFNFEEVIDVLKQRHARGKSFSIVVVSEGAKLQTEEESDKDGSFVLTSMKKDSFGHVRLGGIGNILAEEIEKRTGYETRATILGHIQRGGSPTAYDRVLATRFGVFAVELLVEEKYGYMAALQGSEIVAASLADAVGKLKTVDMKLFEVASTFFG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The Proton acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.2. We require a protein that can specifically bind to the metabolites produced in the glycolysis pathway.3. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in fructose 6-phosphate metabolic process.4. The designed protein should have a binding site that is specific to the target substrate ligand. The designed protein sequence is ",
    "response#1": "CFCMNAAVRAVVRMGIYVGAKVYFIHEGYQGMVDGGENIKEATWESVSSMLQVGGTVIGSARCKEFRTHEGRLRAAHNLVQRGITNLCVIGGDGSLTGANLFREEWSGLLDELLEQGLIDEAAASSNSVLHIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPEMPPEDGWEENMCQKLSESRSRGSRLNIIIVAEGAMDRHGKPVTSDFVKDLVVRCLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVLSTIQEGKWGTMVALSGNKIVDVPLAEGTSVLKTVPLELYEDMQRFFG",
    "response#2": "CFCMNAAVRAVVRMGIYVGAKVYFIHEGYQGMVDGGENIKEATWESVSSMLQVGGTVIGSARCKEFRTHEGRLRAAHNLVQRGITNLCVIGGDGSLTGANLFREEWSGLLDELLEQGLIDEAAASSNSVLHIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPEMPPEDGWEENMCQKLSESRSRGSRLNIIIVAEGAMDRHGKPVTSDFVKDLVVRCLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVLALLEQQPGGRIPLAVVLSGGQAPGGHNVIAGLYDGLKKGNKKSRLLGALGGPKGLIDGKFTEINDDLMDAYRNTGGFDMIGSGRDKIEKPEDLEKVVAVVKANGINGVVIIGGDDSNTNAAVLAEYFQSLKLDVSVVGCPKTIDGDLKNEHIETSFGFDTACKTYSELIGNIGRDANSAKKYWHFIKLMGRSASHITLECALQTQPNVCLIGEEVAAKKQTLDQIVESVASVVAKRAEQGNNFGIVLVPEGLIEFINGHRGVMVALRGNEITPVPLATVVGELKTFPRWSAKVLE",
    "response#3": "CFCMNAAVRAVVRMGIYVGAKVYFIHEGYQGMVDGGENIKEATWESVSSMLQVGGTVIGSARCKEFRTHEGRLRAAHNLVQRGITNLCVIGGDGSLTGANLFREEWSGLLDELLEQGLIDEAAASSNSVLHIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFIPEMPPEDGWEENMCQKLSESRSRGSRLNIIIVAEGAMDRHGKPVTSDFVKDLVVRCLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVLNVAVMNVGAPAAGMNAAVRSAVRVGISEGHKMFAVSDGFEGFYKGQIKEIKWGDVGGWTGQGGSLLGTKRTLPAKHMDKIAEQMRIHNINALLVIGGFEAYLGLVELSSARDKYSEFCVPMVMVPATVSNNIPGSDLSIGADTALNAITDTCDRIKQSASGTKRRVFIIETMGGYCGYLASVGGLAAGADAVYIYEEPFDIRDLQANVEHLTEKMKTSIQRGLVLRNENCNENFTTDFIYQLYSEEGRGVFDCRKNVLGHMQQGGAPSPFDRNFGTKIAAKAMQWVREGYSGTMVALAGIVTCGGLCPGLNDVIRAIVRCLWGRYGVKRISGIRFGYKGLLPDYNFDILPLTPEVIDNCHKTGGSLLGTSRGGGNRVVDIVDGIERLNLHILFIIGGDGSQKGAKEIADEIKHRNLKISIIGIPKTVDNDISFVQKSFGFDTAIVKATEAVAAAHMEARSQINGIGLVKLMGRESGFIATYTAIASHETNFVLIPEVSFDLDGPNGLLAHLEKRIALRKHAVLVVAEGAGQDLMVNADGVPSGDSQGGSLRVSSGTDASGNKRLADIGLFLKEKIGVYFKEKRIHINLKYIDPSYLIRSAVAAPIDSIYCERLGNNAVH"
  },
  {
    "reference": "MSEIQLTQVPSFRRGFRFQWEPAQNCHVLLYPEGMIKLNESAAAVLTEVDGTRTVGAIVADLQARYPEAEGIQEDIVAFLEVAVERFWIELR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be able to regulate pyrroloquinoline quinone biosynthetic process. The designed protein sequence is ",
    "response#1": "HARLLEIGKERYHDKHPFHAMLHGGGCTTTQVRAWVINRYYYQSRIPMKDAAFLSRCDDPDMRRAWRSRIEDHDGGVEEGGGIRRWLRLAEAVGLDPAYVGSARGVLPSTRFAVDAYVSFVREKPLLEAVASSLTELFAPKIHSERIAGLLEHYAFADDAALAYFRQRLAEVPRDVEFGLAYVLDHADTREKQDAAAQALTFKTDVLWAQLR",
    "response#2": "HARLLEIGKERYHDKHPFHAMLHGGGCTTTQVRAWVINRYYYQSRIPMKDAAFLSRCDDPDMRRAWRSRIEDHDGGVEEGGGIRRWLRLAEAVGLDPAYVGSARGVLPSTRFAVDAYVSFVREKPLLEAVASSLTELFAPKIHSERIAGLLEHYAFADDAALAYFRQRLAEVPRDVEFGLAYVLDHADTREKQDAAAQALTFKTDVLWAQLR",
    "response#3": "NWILCNASPDIRAQIEAFPALQPARAVRDTAIRALILLDSQIDHCTGLLGLREGCPHEVWCTEMVHQDLTTGFPLFAMLEHWNGGLHWKPIVLERSFAVDACPALRFTPIPLRSAAPPYSPHRHDPHPGDNLGLLVEDSRTGGTLFYAPGLGRVDAPLRERMRRADCLLVDGTLWRDDEMIHAGCGSKLGSEMGHLPQSGAGGMLEVLDGLDGRKVLIHHARLLEIGKERYHDKHPFHAMLHGGGCTTTQVRAWVINRYYYQSRIPMKDAAFLSRCDDPDMRRAWRSRIEDHDGGVEEGGGIRRWLRLAEAVGLDPAYVGSARGVLPSTRFAVDAYVSFVREKPLLEAVASSLTELFAPKIHSERIAGLLEHYAFADDAALAYFRQRLAEVPRDVEFGLAYVLDHADTREKQDAAAQALTFKTDVLWAQLL"
  },
  {
    "reference": "MAERVLVVGSGGREHALAWKLAQSPQIQQVLVAPGNAGTASCGKISNSEVSVNNHSILAQFCKDHHVGLVVVGPEVPLAAGIVDDLMAAGVPCFGPSAKAAQLEASKSFSKAFMERHGIPTARYGSFTDPQEACNYIRTADFPALVVKASGLAAGKGVIVAKDQDEACQAVMDIMKDRAFGAAGETVVVEELLEGEEVSCLCFSDGSSVSPMPPAQDHKRLQDGDLGPNTGGMGAYCPTPQVSQELLQQIRETVLQKTVDGMKEEGTPYVGVLYAGLMLTKQGPKVLEFNCRFGDPECQVLLPLLKSDLYEVILNTMNGKLASSAPMWHQDSSAVTVVMASAGYPGSYKKGVEITGLSQVQDKGLQVFHAGTALKEGGGGVVSSGGRVLTVTAISSSLEAALQAANQGVAAIGFPGAEYRRDIGHRAIAHLNQRRGLTYKDSGVDIAAGNKLVEMIKPLAKATSRTGCNAELGGFAGLFDLKAAGFVDPILVSGTDGVGTKLKIAQECRQHGSLGQDLVAMCVNDVLAQGAEPLFFLDYFSCGSLDVDVAASVVGGVAKACEMAGCALLGGETAEMPGVYAPGEYDLAGFCVGAVERGALLPRLGDIAEGDLLIGLASSGVHSNGFSLVRKVLEKAHLNYSSPAPFGKPEQTVGEALLTPTKIYSRLLLPILRSGAVKAYAHITGGGLLENIPRVLPQELAVDLDASRWSIPPVFSWLHKEGGLSEEEMARTFNCGLGAVLVVAPLDAQRVLRQLQAHEEAWIVGSLA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein should be able to manipulate the activity of enzymes in the N(2)- formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D- ribosyl)glycinamide (10-formyl THF route): step 1/1, IMP biosynthesis via de novo pathway, Purine metabolism pathway, either by activating or inhibiting their function.2. The presence of ATP-grasp domains is critical for the interaction of the designed protein with its intended targets.3. The designed protein should be optimized for phosphoribosylglycinamide formyltransferase activity, phosphoribosylamine-glycine ligase activity, ATP binding, metal ion binding activity in order to improve purine nucleobase biosynthetic process, 'de novo' IMP biosynthetic process, camera-type eye development, pigmentation, chordate embryonic development. The designed protein sequence is ",
    "response#1": "AEEVGVKTSQYRFVKTLDELRSAAEEIGYPCVIKPVMSSSGHGQSVCRMAEEIEACFEEAKEARGDSSELIVEEFIPFDYEITLLTLNNGKKITFCDPIGHIQKGGDYIFSWQPAQMKKSVLKKAQKIARDVVSALGGRGIFGVELFIKKNEVYFSEVSPRPHDTGMVTLITQNFSEAVGVILASRGYPASSSKDQVITGTDEVIRKQDYFVFHSGTTVSSEGELVTNGGRVLITVSIAPTLAMAAARATQAAEYISFDGKQFRTDIAHFCVGVVMASRGYPLTSSKGDIISGLPSVTKGMVFHMGTKLNNYGEVVTNGGRVLIVVTTHQDLVLAAARATMACGRIIFNGAQFRTDIAHRGTAITVVMASKGYPGDYTKGVEITGFPEAQALGLEVFHAGTVLKDGKVVTNGGRVLTVTAIRENLNVALEEAKKGLAAIKFEGAIYRKDIGYRAFCVGVVMASRGYPLTSSKGDIISGLPSVTKGMVFHMGTKLNNYGEVVTNGGRVLIVVTTHQDLVLAAARATMACGRIIFNGAQFRTDIAHRGV",
    "response#2": "KQFMDRNHIPTARWQGFTSAINAKNFIKNAPFKALVIKASGLAAGKGVVVAKDQQEACYAIDEMLNDKKFGVAGETVVVEELLEGEEVSVLAFTDGKTLVPMMPAQDHKRIFNGDFGPNTGGMGAYCPCPLLSEVEYEEVKINVLQKAVDGLRKENIPFVGVLYAGLMLTEDGPKVLEFNCRFGDPETEVILPLLASDLFTIMKACCSDKEWSKEFMNEFNIPTAKWRSFNDVKQATDFVYSADYPALVVKASGLAAGKGVVVASNKQEACLALDAILTENRFGKAGQTVVVEELLQGEEVSVLCFTDGVNINVMLPSQDYKRAYNQDKGPNTGGMGAFCPCYNMPQNELDEAKNILQRAVDGLRNRGTPFVGVLYAGLMVTNDGIRVLEFNCRFGDPECVGVVMASRGYPLTSSKGDIISGLPSVTKGMVFHMGTKLNNYGEVVTNGGRVLIVVTTHQDLVLAAARATMACGRIIFNGAQFRTDIAHCVGVVMASRGYPLTSSKGDIISGLPSVTKGMVFHMGTKLNNYGEVVTNGGRVLIVVTTHQDLVLAAARATMACGRIIFNGAQFRTDIAHFGSSKRFAKEFMDRHGIPTARWRAFTKPEEACSFIMSSDFPALVVKASGLAAGKGVIVAKSTEEACRAVQEIMQVGCSSGKKQLSLKELLEGEEVSCLCFTDGRTVAPMPPAQDHKRLLEGDHGPNTGGMGAIVQHLRYFPEALLKSLVIIKFFLKELLDPYFFCVLYAGIMLTKDGPKVLEFNCRFGDPE",
    "response#3": "AAEELDLPTSPYHFADTFEDYSKAVADLGYPCVVKPVMSSSGKGQSLLRSADDLQKAWDYAQEGGRAGKGRVIIEGFIDFEYEITLLTVRHVGGTTFLEPVGHRQEKGDYQESWQPQAMSPKALAESQRVAQAVTDALGGRGLFGVELFVKGDQVWFSEVSPRPHDTGLVTLISQELSQLTHPATTAITVVMASKGYPGDYTKGVEITGFPEAQALGLEVFHAGTVLKDGKVVTNGGRVLTVTAIRENLNVALEEAKKGLAAIKFEGAIYRKDIGYRAFCVGVVMASRGYPLTSSKGDIISGLPSVTKGMVFHMGTKLNNYGEVVTNGGRVLIVVTTHQDLVLAAARATMACGRIIFNGAQFRTDIAHRGFCVGVVMASRGYPLTSSKGDIISGLPSVTKGMVFHMGTKLNNYGEVVTNGGRVLIVVTTHQDLVLAAARATMACGRIIFNGAQFRTDIAHRGRVPNAITVVMASKGYPGDYTKGVEITGFPEAQALGLEVFHAGTVLKDGKVVTNGGRVLTVTAIRENLNVALEEAKKGLAAIKFEGAIYRKDIGYSVCKYLVPPGYPEKGERGLVVTVPAEAAGTECFLGSLALQGDHLVTEGSRTLAVTGFGNTLDEAEARAESWIASVGGGLVHRGDIGRFCVGVVMASRGYPLTSSKGDIISGLPSVTKGMVFHMGTKLNNYGEVVTNGGRVLIVVTTHQDLVLAAARATMACGRIIFNGAQFRTDIAHRGRVPNTVLGGL"
  },
  {
    "reference": "MNPLINFILLSSMIAGTILTTTSHHWVSAWLGLELNTLAIIPIISMTHHPRATEASTKYFLIQAASSALVLLSGIINAHLHGSWDISQLSDNFSKIALTTALATKLGLAPIHFWLPEILQGVPILTALIIATWQKIAPMALLIMIWDLIPTPITLIMGLTSTIVGGLGGLNQTQLRKTMAFSSIAHLGWMITMMTIAPNLTLLNLILYIPMTSLTMLIMHLTMSKTLQNAMLIPSHSLTATSLFLLSLLSLGGLPPLSGFIPKWLILQELTIHNLTPMAFMMAITALLSLMFYLRITYISTMTLPPSTTPLKNTWRFKSHQNTSTLPLLSLISIFLLPITPTLTQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis; Complex I functions in the transfer of electrons from NADH to the respiratory chain; The immediate electron acceptor for the enzyme is believed to be ubiquinone .2. The protein should be optimized for stability and expression in respirasome, mitochondrial inner membrane where it will participate in mitochondrial electron transport, NADH to ubiquinone. The designed protein sequence is ",
    "response#1": "YSTQIMLFTIMEHPRTHHTTHKETKNITHPLARLMLTSILMGTMTKMSTLQTTTMVTMPKTIKLMALISTIIGVLLSKDLTHMTHHMKPKKPNKQNMFFNQLAFFNIPHRTITINTLKISQQTSTELMDLWTLEVWGPKGLSNTITNTIHLLTQQKNMIKNYMAIFTMTTMTVLLFN",
    "response#2": "YSTQIMLFTIMEHPRTHHTTHKETKNITHPLARLMLTSILMGTMTKMSTLQTTTMVTMPKTIKLMALISTIIGVLLSKDLTHMTHHMKPKKPNKQNMFFNQLAFFNIPHRTITINTLKISQQTSTELMDLWTLEVWGPKGLSNTITNTIHLLTQQKNMIKNYMAIFTMTTMTVLLFG",
    "response#3": "YSTQIMLFTIMEHPRTHHTTHKETKNITHPLARLMLTSILMGTMTKMSTLQTTTMVTMPKTIKLMALISTIIGVLLSKDLTHMTHHMKPKKPNKQNMFFNQLAFFNIPHRTITINTLKISQQTSTELMDLWTLEVWGPKGLSNTITNTIHLLTQQKNMIKNYMAIFTMTTMTVLLFT"
  },
  {
    "reference": "MAFVPDLDTLESNSLNEEMLYGPNCLCPQKKPRLDLEVTSPGVGIQVTVTKGHLSRTFRQAAVLVAAVTKLLKQPSHNDFSDSDLGSFFDDIFEPVSFQCIKGSYTKAPVFRYTRSQSFDILDVDHKCFVLESPTQLVALHLQGPSAGRKVKLDIALYRPRSSQGGPRSGRVPVALGIKGYQLYMSCVMSDTQPVLQLEEADIRRDLESVELTRFIFYRLDSPAEGTTRFESAAFPGWFVCTSLQPRQPVGITNTPDQVNIATYELSGH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that can perform protein domain specific binding, cytokine activity, interleukin-1 receptor binding, integrin binding in cytosol, extracellular space to support positive regulation of interleukin-2 production, positive regulation of glial cell proliferation, defense response to Gram-positive bacterium, interleukin-1-mediated signaling pathway, positive regulation of interleukin-8 production.2. The protein contains novel Interleukin-1 propeptide domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "PLVSYLQISALSSTGLLVCPDLKEFISSRTDGKIQWYKGSILLDKGNKKFLSAGDPTRLLISNTSMGDAGYYRCVMTFTYEGKEYNIC",
    "response#2": "PVTSEKHPVLGDDCWFRGRDFKSELRLEGEPVVLRCPLVPHSDTSSSSRSLLTWSKSDSSQLIPGDEPRMWVKDDTLWVLPAVQQDSGTYICTFRNASHCEQMSLELC",
    "response#3": "PPKNLQLKPLRGSQMELSWEYPDSWSTPHSYFSLKFHVQVHRKRERKDESQFVDKTSATIRCSKGAEVRVRAQDHYYNSSWSRWVSVPCR"
  },
  {
    "reference": "MGGKWSKSSIIGWSTIRERMRRAEPAAEGVGAASRDLERHGALTSSNTVANNPTCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSRKRQDILDLWVYHTQGYFPDWQNYTPGPGTRYPLCFGWCFKLVPAEPEDGENSSLLHPVSQHGIEDSEREVLKWKFDSHLAFQHKARELYPEYYKD",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should include a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Diacidic; necessary for CD4 internalization motif to ensure proper function.2. For general function, the protein need meet that Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK13. A protein that can perform GTP binding in extracellular region, virion component, host cell Golgi membrane, host cell plasma membrane to support suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host autophagy. The designed protein sequence is ",
    "response#1": "MGGKWSKCSIVGWPAIRERIRRTEPAAEGVGAASRDLDKYGAITSSNTVTNNAACAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLVYSQQRQDILDLWVYHTQGYFPDWQNYTPGPGTRYPLTFGWCFKLVPVEPEKVEEANTGENNCLLHPMSQHGMDDSEREVLKWKFDSRLAFHHMARELHPEYYKDC",
    "response#2": "MGGKWSKRNMEGWPAVRERMERAEPAAEGVGAVSRDLERHGAITSSNTAQTNPDCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWIYNTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPLEPDEVEKANEGENNCLLHPMSQHGMDDPERETLMWKFDSRLAFHHVAREQHPEYYKNC",
    "response#3": "MGGKWSKSSIVGWPQIRERIRQTPPAAEGVGAASQDLDKYGALTSSNTPTNNAACAWLEAQEEEEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLDGLIYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPREVEKANEGENNCLLHPISQHGMEDEDREVLMWKFDSSLARRHLARELHPEFYKDC"
  },
  {
    "reference": "MNYQNSLVFALEQDTHDELRDFRSRFLIPQHHGADAIYLCGNSLGLQPVSAAKHITDQLSNWQNLAVEGWFQGDDPWLDYHKQLLPSISRIVGAQENEVAVMNSLTVNLHLLMVSFYKPNSKRFKIIMEGGAFPSDQYAMESQVRFHGFDPKEAIIELFPREGELTLRTEDIIQKINEHAHELALVLFGGINYYTGQLFDMEAIADAAHQASAYAGFDLAHASGNVPLKLHDWGADFACWCSYKYMNSGPGGISGIFVHEKHSTNNDLDRFAGWWGYREDKRFLMAPGFDAAKGAAGWQVSTSPILLMALFKASMVIFDDTGGIDTLRAKSMALTGYMEFLINEINKERGEDVFKIITPANPRERGCQLSVVCRQNAKAVFKYLADNGVIGDWREPDVIRLSPVPLYNTFQDVYRAAQQLAAATNNLQYGLL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must be engineered to have a high affinity for pyridoxal 5'-phosphate in order to increase its catalytic activity.2. A protein with kynureninase activity is needed to enhance L-kynurenine catabolic process, anthranilate metabolic process, 'de novo' NAD biosynthetic process from tryptophan, tryptophan catabolic process in a specific context.3. Ideal protein should have a binding affinity for key enzymes in the NAD(+) biosynthesis pathway to enhance the overall efficiency of the pathway.4. For general function, the protein need meet that Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively.5. The protein have enhanced H2O + L-kynurenine = anthranilate + H(+) + L-alanine.6. The designed protein have Aminotransferase class V domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "STHALISQLEARGMNPAEDLIFVGATQEENWESSSVNIPIEQFHSVVEKCGDEIAVAVLSGVQFFTGQLFDIQAITKAAHAKGILVGVDCAHAVGNVPLQLHDWEVDFACWCNYRYLNSGPGNLGGVFVHSKHTSVENELRPLRGWWGHDCRSCYSLQHSFEPAEGAAGFQISNVPALGMMAMLPSVRLMAEVGMGPLREKSLLLTSYMELLLGSTHALISQLEARGMNPAEDLIFVGATQEENWESSSVNIPIEQFHSVVEKCGDEIAVAVLSGVQFFTGQLFDIQAITKAAHAKGILVGVDCAHAVGNVPLQLHDWEVDFACWCNYRYLNSGPGNLGGVFVHSKHTSVENELRPLRGWWGHDCRSCYSLQHSFEPAEGAAGFQISNVPALGMMAMLPSVRLMAEVGMGPLREKSLLLTSYMELLLGTRGADVYGSTHALISQLEARGMNPAEDLIFVGATQEENWESSSVNIPIEQFHSVVEKCGDEIAVAVLSGVQFFTGQLFDIQAITKAAHAKGILVGVDCAHAVGNVPLQLHDWEVDFACWCNYRYLNSGPGNLGGVFVHSKHTSVENELRPLRGWWGHDCRSCYSLQHSFEPAEGAAGFQISNVPALGMMAMLPSVRLMAEVGMGPLREKSLLLTSYMELLLGARGADV",
    "response#2": "MSATKQQSAKIVGALPSEVVVMETLTANLHLLMSSFYRPTADRWKIIIEGKAFPSDHYAALSQIAHHNLDPSALITIEPPSASSPYLSTEHILSVISKHAATTALVLLPGIQFYSGQFLDIELITRHCHSNGIPIGWDLAHAVGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHEEYTVKSTHALISQLEARGMNPAEDLIFVGATQEENWESSSVNIPIEQFHSVVEKCGDEIAVAVLSGVQFFTGQLFDIQAITKAAHAKGILVGVDCAHAVGNVPLQLHDWEVDFACWCNYRYLNSGPGNLGGVFVHSKHTSVENELRPLRGWWGHDCRSCYSLQHSFEPAEGAAGFQISNVPALGMMAMLPSVRLMAEVGMGPLREKSLLLTSYMELLLGAIKEQTAKILGALPTEVVIMETLTANLHLLMSSFYRPTKDRWKIIIEGKAFPSDHYAVLSQIANHSLDPSALITIDPPSASSPYLSNEHILSVISRHAATTALVLLPGIQFYSGQFFDIELITRHCQSLGITVGWDLAHAAGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHYHYGYINAIKEQTAKILGALPTEVVIMETLTANLHLLMSSFYRPTKDRWKIIIEGKAFPSDHYAVLSQIANHSLDPSALITIDPPSASSPYLSNEHILSVISRHAATTALVLLPGIQFYSGQFFDIELITRHCQSLGITVGWDLAHAAGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVH",
    "response#3": "MATKQQSAKIVGALPSEVVVMETLTANLHLLMSSFYRPTADRWKIIIEGKAFPSDHYAALSQIAHHNLDPSALITIEPPSASSPYLSTEHILSVISKHAATTALVLLPGIQFYSGQFLDIELITRHCHSNGIPIGWDLAHAVGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHEGRLYREGFDAIKEQTAKILGALPTEVVIMETLTANLHLLMSSFYRPTKDRWKIIIEGKAFPSDHYAVLSQIANHSLDPSALITIDPPSASSPYLSNEHILSVISRHAATTALVLLPGIQFYSGQFFDIELITRHCQSLGITVGWDLAHAAGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHGRLDRGVLEMAIKEQTAKILGALPTEVVIMETLTANLHLLMSSFYRPTKDRWKIIIEGKAFPSDHYAVLSQIANHSLDPSALITIDPPSASSPYLSNEHILSVISRHAATTALVLLPGIQFYSGQFFDIELITRHCQSLGITVGWDLAHAAGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHGRLDKGVLENVATKQQSAKIVGALPSEVVVMETLTANLHLLMSSFYRPTADRWKIIIEGKAFPSDHYAALSQIAHHNLDPSALITIEPPSASSPYLSTEHILSVISKHAATTALVLLPGIQFYSGQFLDIELITRHCHSNGIPIGWDLAHAVGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHE"
  },
  {
    "reference": "MSVTRLSFPYGESIQWHSPDQIPQLPPSPFREWLLASGSLTQKLKSHCNQFEVKVLGEAMLPPFPGELPHQGQAWIREVLLCLDGIPWVFARTLVPGAMMDSAQDNFLSLGTRPLGELLFTSGDFTPGKIEVGEFTACDSLAKLIDSLEQESHHPLWGRRRYFSHGDQQLIVSEIFLPKARQLIDRLA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be able to regulate ubiquinone biosynthetic process, pyruvate biosynthetic process. The designed protein sequence is ",
    "response#1": "MPQF",
    "response#2": "MF",
    "response#3": "GTLSSFI"
  },
  {
    "reference": "MSSDDQHIREIRVHPIENGTVIDHINAGQALNVLKILNIPTSSSRVVSVLINAPSVHGRKDVVKIEGRELNVEEVDKIALIAPNATINIIRDFEVSDKDIVHIHSQIEGVVRCINPNCISNSNEPVTSKFAVSSNGQRTILRCSYCERIISDDIGEHLL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Involved in allosteric regulation of aspartate carbamoyltransferase.. The designed protein sequence is ",
    "response#1": "GLKVQAIESGTVIDHIKSGQALNVLRILGISSAFRATISFVMNAPGAGGKKDVVKIEGKELSVEELNRIALISPKATINIIRDFVVVQKNNE",
    "response#2": "MKILKVQAIESGTVIDHIKSGQALNVLRILGISSAFRATISFVMNAPGAGGKKDVVKIEGKELSVEELNRIALISPKATINIIRDFVVVQKNNG",
    "response#3": "VEGVVRCTNSSCISNSSEPIKSKFSVIQSEEEGVSLHCLYCEHVISER"
  },
  {
    "reference": "MEQGRPRSSLSLTSSASTVSSLSSLSAKKPTRVVHKVHAFGKRNNALRRDPNLPVHIRGWLHKQDSSGLRLWKRRWFVLSGHCLFYYKDSREESVLGSVLLPSYSVRPDGPGAPRGRRFTFTAEHPGMRTYVLAADTLEDLRGWLRALGKASRAEGEDCGLPRSPARPRPGEGPGGPGGPPEVNRREEGRTSESPEVAPLSRGPGRHEMHAPGSSADLQPDTWSRRTRSPEPFSPLSRPPSPLSLPRPRSAPVRRPPLSAGDISFPARPHTPLSRIDVRPPLDWGPQRQTLSRPPIPRRGPFSEAGGGRPPRSPQLRTPEHRTQSTQVSSGSSTYLQLPPRPPGTQASMILLPGPPVDSTLHQSLETDTLLTKLCGQDRLLRQLQEDLDKRQEEKEQLEAALELTRQQLGQATREAAASGKAWGRQRLLQDRLVNVRAALCHLTQERERVWDTYSGLEQDLGTLRETLEYLLHLGSPQDRASAQQQLWMVEDTLAGLGGPQKQPPHTEPKSPSPAPQREESSERESLSESLELSSPQSPEVDWGQPPGGDRALSSSQSGVGSPRVSRASSPECRQQSSPLLRTKAPLARPRMSAQEQLERMRRNQACGLSLPRPTSPRLLTLGRTLSPVPRQPDMEQRPIVGAAKWLRSSGSWSSPRHSTTYSPVSGGHRERVLSLSQALATEASQWHRLMTASPERNLDTRGDCLQPSPQPPSEELPQVTSSPTSHKANSATTGFSCQGSGRGLAPWEPRWDPGKAPPALAQEEGAW",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should contain one or more PH domains that are essential for its function.2. The designed protein should be involved in positive regulation of Wnt signaling pathway, planar cell polarity pathway by localizing to extrinsic component of cytoplasmic side of plasma membrane, cytoplasm and exhibiting phosphatidylinositol-4,5-bisphosphate binding, phosphatidylinositol-3,4-bisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding.3. For general function, the protein need meet that Binds specifically to phosphatidylinositol 3-phosphate (PtdIns3P), but not to other phosphoinositides.4. The designed protein's functionality will benefit from Pro residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "KMDFLQIMLNSVHSIPEHWQSQYSEFYMSLDLYHGTQVLDGFSNKVPKTIKNDHFFPRIPLDLYAKFKRLNLCQYPRETRIVVSISGTVRNSAQAANEYNPDIVMLGYCSVPLYDENLFMRQGPLFLPLTLLKKQPMLKPFGPYPYIKDARDPILIMSFKIWDTEIYFP",
    "response#2": "LMGFIKLKLNRYAIIANTVEETGRFNGHVFYRVLQHSIVSTKFNSRIDSEEAEYIKLLELHLKNSTFYFSYTYDLTNSLQRNEKVGPAASWKTADERFFWNHYLTEDLRNFAHQDPRIDSFIQPVIYGYAKTVDAVLNATPIVLGLITRRSIFRAGTRYFRRGVDKDGNVGNFNETEQILLAENPESEKIHVFSFLQTRGSVPIYWAEINNLKYKPNLVLGENSLDATKKHFDQQKELYGDNYLVNLVNQKGHELPVKEGYESVVHALNDPKIHYVYFDFHHECQ",
    "response#3": "KMDFLQIMLNSVHSIPEHWQSQYSEFYMSLDLYHGTQVLDGFSNKVPKTIKNDHFFPRIPLDLYAKFKRLNLCQYPRETRIVVSISGTVRNSAQAANEYNPDIVMLGYCSVPLYDENLFMRQGPLFLPLTLLKKQPMLKPFGPYPYIKDARDPILIMSFKIWDTEIYFQ"
  },
  {
    "reference": "MDDSIDEEVISGGSTITVEFSGGAETLFGGVKEHIVPLDGSKIVLLEEMLRWLRDHLLTGDAGLFLQENTVRPGILVMINDTDWDLMGETEYILQPGDHILFISTLHGG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be involved in tRNA wobble uridine modification, protein urmylation by localizing to nucleus and exhibiting protein tag.2. The protein must exhibit the following characteristics: The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.; Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3; Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln); The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. The designed protein sequence is ",
    "response#1": "VAVGASGGKDSTVLAHVLRELAPRLGITLHLVAVDEGIGGYRDAALEAVRSQAARWELPLTIVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARLVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGVLGSTGEGCALPRCRPLQFASQKEVVLYAHFRHLRYFSEELGEHILLDTREKEHFSFGSIPGAVNVPFSKFLVKASSIKREGNSPAELLPMQPASDEAPIVVVCRRGQDSQEVVEKLKELGLDNGGKRKIMDIVGGMKAWRS",
    "response#2": "ELGEHILLDTREKEHFSFGSIPGAVNVPFSKFLVKASSIKREGNSPAELLPMQPASDEAPIVVVCRRGQDSQEVVEKLKELGLDNGGKRKIMDIVGGMKAWRDEVDPDFL",
    "response#3": "FEEEIHHTIVSAKLFNPGEKVGIGASGGKDSTVLAHVLKVLNERYAYGLDLILVSVDEGISGYRDDSLETVKRNQQQYELPLKIVSYQELYGWTMDQIVKQVGLKNNCTFCGVFRRQALDRGAMMLGVNKICTGHNADDIAETVLMNFLRGDIARLRRCTSITTGSEGAIPRCKPLKYAYEKEIVLYAYFKKLDYFSTECIYSPNYELPCLGEHILLDTREKEHFSFGSIPGAVNVPFSKFLVKASSIKREGNSPAELLPMQPASDEAPIVVVCRRGQDSQEVVEKLKELGLDNGGKRKIMDIVGGMKAWRP"
  },
  {
    "reference": "MAKYLFITGGVVSSLGKGITAASVGRLLINRGLKIRMLKMDPYLNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERYTGAPTSQKSNITAGSIYWDVLHKERRGDYLGGTIQMIPHISNEIKARIKVLEEDNPDVILCELGGTVGDIEGLIFLEALRQLALEVGRDNSMFIHLTYVPYIAAAKEVKTKPTQQSVAKLREIGIMPDVLVCRTEVDMEREHLEKLSLFCNVPKDCVIVEKDVETSIYEIPLVLSRAGLDEIILNRFRIAKPSRPLTKWEKLIETIRHPKGTVRIGVVGKYSELQDAYKSIYEALYHGGYAAGYKVDAVKVAAEDIEDDPSVLDTVDGVLIPGGFGSRGMEGKIRAAQYARENKIPFLGICLGLQCAVIEFARNVCGLEDAHTTEFDEERGVKTTHPVVCLMEEQTEVQEKGGTMRLGACPCDLKEGSKSFAAYGKKRVSERHRHRYEVNNRYREQLEANGLILSGINPDIGVVEMIELADHPWYVATQAHPELKSQPVDPHPLFKDFVAASVARMKEQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be able to perform ATP binding under specific conditions.2. The protein should be able to bind ATP ligand in a variety of conditions3. The designed protein should contain one or more CTP synthase N-terminal domains that are essential for its function.4. The designed protein must have a Nucleophile that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "LHDAYLSVAESLNHASAANDAVMEIKWVDSEELMEDNIASVLGDCSGILVPGGFGDRGIEGMILAAKYARENDVPYLGICLGLQIAVIEFARHVLGWKDANSAEFTADSQHLVIDLMPEQQGITAKGGTMRLGKYPCVLSPESKAYEYYGEGLIYERHRHRYEVNNDFREEIVARGLLPVGMSPDGSLVEMVENPACKWFVAAQFHPEFKSRPNKPHPLFNGFVEDSYVSVIEAIKHASGNKKVVPEIVWMDSKDFEKDENKLQKLNDIDGVIVPGGFGSTGIEGKILVAKYCRENNVPYLGLCLGLQIAVIEFARNVCGLEGSNSTEFDKNTKHPSVCILPDQVEKMKKSQYGGTMRLGGQDVKIVENTIAEKIYGKNLIRERFRHRFEINPELIENLKNKGLVFSGMTPDNKIMQIIELSEHIFFMGSQFHPEFTSRPINPNPLFTKFIEWSGG",
    "response#2": "LPDAYLSVVEALRHAASYHGVEVDIEWIHSEEIEGLLAAGRLRHLDGMVIPGGFGSRGIEGKIAAAAYSREHDIPCLGLCLGLQVMVIEFCRNELGLLGANSTEFDANTLHPVIDLMHAQRGIEDKGGTMRLGAYVARLAPRSRVAAMYGDTLVNERHRHRYEVNSAYRQRMEEGGLQCSGLSPDGRLAEFIELDGHAFWVGTQAHPEFKSRPNRPAPLFCGY",
    "response#3": "LPDSYKSLAEALDHGGIANKVAVDLQFIDAAGLEEKSDAEIAALFSPLHAILVPGGFGDRGTEGKIAAVRFARERKIPYFGICLGMQLAVIEAARNLAGLDGAGSTEFGDCAVPVVGLLTEWIKDDGAKEQRGEEDDKGGTMRLGAYQTQLAPESLLSEIYGGTEISERHRHRYEVNTTYRAKLEEAGLIFSGMSPDGVLPETVERSDHPWFVGVQFHPELKSRPFAPHPLFVSFLPDAYKSVNEALKHGGLKNRLSVTIKYVDSQDVEAKGTSILEGLDGILVPGGFGERGVEGKIMAARYARENKVPYLGICLGMQVALIEFARNVAGLKDAHSSEFMKQTPHPVVGLITEWVDEEGNVEERSEDADLGGTMRLGAQLCHLKPGSKVAELYGGETCVERHRHRYEVNNNYRKQLEEAGLSFAGLSTDKKLVEIIEIPQHPWFVAGQFHPEFTSTPRDGHPLFAGFIRS"
  },
  {
    "reference": "MAEIRNYTMNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLHRGTEKLAENRTYLQSVPYMDRLDYVSMMVNEHAYVMAIEKLLGLEVPLRAQYIRVMFDEITRILNHLLWLGAHALDVGAMTVFLYAFREREDLFDCYEAVSGARMHAAYYRPGGVYRDLPDRMPQYEESTVRSKEDVKQLNENRQGSLLDFIEDFTNRFPAYVDEYETLLTDNRIWKQRTVGIGVVSPERAMALGMTGPMLRGSGVAWDLRKKQPYEVYDRLDFDIPIGVNGDCYDRYLVRIEEFRQSNRIIKQCIDWLRKNPGPVISDNTKVAPPPREEMKHDMEALIHHFKLFTEGFHVPAGEAYAAVEHPKGEFGIYLISDGANKPYRLKIRAPGFAHLAALDEMTRGHMIADLVAIIGTQDIVFGEIDR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain2. The protein must exhibit NAD binding.3. The protein have enhanced a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) +   NAD(+). The designed protein sequence is ",
    "response#1": "RIKRPMIRRDGTLTPVSWEEAGDFLRQRLGVAEGKAAGGLISPRLPNEVLYQFQKLMRTVLRTNNVDCSSRWSAPLDILVPIVASFYSRDPLEQVIGKDCVLIIGGNVTEENPVTEYLLRDAARRRHTRLLMLSARPSRLDADARAVLRAHPGGEGQSLAAVVAALVAVTDEGLPDDIFAKTSGTTASSGANDALDRLVSTLKEGRSVTLLVSVDLLRSPLARKTLEQLGNLLQLLRLLGKEPSLQFLFDRANQMGAWDMGVLPGVLPGLSPIADEATRTRFERSWGAEIPREPGADVDAMLELCEKGGMGVLYVVGSDPLISYPDREFVERALGAANLLIVQDAFLTDTAGLADVVLPAAGYGEESGTFTNNEGRTQALRKFREPAFDARSNLAIFGFGRAGL",
    "response#2": "RIKRPMIRRDGTLTPVSWEEAGDFLRQRLGVAEGKAAGGLISPRLPNEVLYQFQKLMRTVLRTNNVDCSSRWSAPLDILVPIVASFYSRDPLEQVIGKDCVLIIGGNVTEENPVTEYLLRDAARRRHTRLLMLSARPSRLDADARAVLRAHPGGEGQSLAAVVAALVAVTDEGLPDDIFAKTSGTTASSGANDALDRLVSTLKEGRSVTLLVSVDLLRSPLARKTLEQLGNLLQLLRLLGKEPSLQFLFDRANQMGAWDMGVLPGVLPGLSPIADEATRTRFERSWGAEIPREPGADVDAMLELCEKGGMGVLYVVGSDPLISYPDREFVERALGAANLLIVQDAFLTDTAGLADVVLPAAGYGEESGTFTNNEGRTQALRKFREPAFDARSNLAIFGFG",
    "response#3": "RIKRPMIRRDGTLTPVSWEEAGDFLRQRLGVAEGKAAGGLISPRLPNEVLYQFQKLMRTVLRTNNVDCSSRWSAPLDILVPIVASFYSRDPLEQVIGKDCVLIIGGNVTEENPVTEYLLRDAARRRHTRLLMLSARPSRLDADARAVLRAHPGGEGQSLAAVVAALVAVTDEGLPDDIFAKTSGTTASSGANDALDRLVSTLKEGRSVTLLVSVDLLRSPLARKTLEQLGNLLQLLRLLGKEPSLQFLFDRANQMGAWDMGVLPGVLPGLSPIADEATRTRFERSWGAEIPREPGADVDAMLELCEKGGMGVLYVVGSDPLISYPDREFVERALGAANLLIVQDAFLTDTAGLADVVLPAAGYGEESGTFTNNEGRTQALRKFREPAFDARSNLAIFGFGPRMQGLTPRIKRPMIRRDGTLTPVSWEEAGDFLRQRLGVAEGKAAGGLISPRLPNEVLYQFQKLMRTVLRTNNVDCSSRWSAPLDILVPIVASFYSRDPLEQVIGKDCVLIIGGNVTEENPVTEYLLRDAARRRHTRLLMLSARPSRLDADARAVLRAHPGGEGQSLAAVVAALVAVTDEGLPDDIFAKTSGTTASSGANDALDRLVSTLKEGRSVTLLVSVDLLRSPLARKTLEQLGNLLQLLRLLGKEPSLQFLFDRANQMGAWDMGVLPGVLPGLSPIADEATRTRFERSWGAEIPREPGADVDAMLELCEKGGMGVLYVVGSDPLISYPDREFVERALGAANLLIVQDAFLTDTAGLADVVLPAAGYGEESGTFTNNEGRTQALRKFREPAFDARSNLAIFGF"
  },
  {
    "reference": "MAAEPPARQRRSRVLLIVAGGIAAYKAPDLVRKLIAAGCEVQVVVTEAALGFCTELSLATVSGRPVRRSLLDAGAETGGVGHIELADWPDLVLIAPATADLMARAAAGMANDLASCVLLATRAALLWAPAMNTNMWRHPATRHNLELLAARGSSFVGPDRGELACGWIGEGRMLDPPILAAAARATLDGEVHPQVRGPEAGSATASVTSSALSGRRVVVTAGPTRAFLDPVRFISNASTGAMGFALAAAARARGAEVVLIAGPVALATPAGVRRVDVETGAQMLAACREAVDEGADLLAMVAAVADLVPADPATEKVDKDRVLAGFADMQWRAEVDILATLVREREAGERGVGMRILGFGAQTVASRPGEGETSEGETSEGETGEGEPGAGAAEADRVRARLRELGRAKLARKGCDALFVNRVGIPGGGFASPTNAGLLIFSDGPGAGGPGAGGPGELDSGPSRPKAELAAWMLDGLAARWWSAAQEGEGA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have a high affinity for CTP.2. The presence of Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.3. The protein should be designed to catalyze the following reaction H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. A protein should be able to manipulate the activity of enzymes in the CoA from (R)- pantothenate: step 3/5 pathway, either by activating or inhibiting their function.5. A protein with metal ion binding, phosphopantothenate--cysteine ligase activity is needed to enhance pantothenate catabolic process, coenzyme A biosynthetic process in a specific context.6. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MRILLGVAGGIAAYKAVLLLRLLREAGHDVRVIPTHAALEFVGRPTWEALSGHPATPEVFEDVEHVPHVSLGQGADLLVVAPATADLLSRAATGRADDLLTSTLLTVRCPVLLAPAMHTEMWDHPATRANVATLRQRGVHVLEPASGRLTGSDTGPGRLPEPAEIAAAALALLGAAGHGLAGRHVVVSAGGTREPIDPVRFLGNRSSGRQGLALARAARARGARVTLVAANVTLPDPDGVDVVHVESTAELRDAVHAAAKDADVVVMAAAVADFRPASTAGHKIKKRDDGVVAPIALEANPDVLAELAGELRRPGQVVVGFAAETGDDAGDVLEHGRHKARRKGADLLAVNAVGGGLGFGTADNEVTILTADGETPVPRGPKEALADVVWDLVVTRLD",
    "response#2": "MRILLGVAGGIAAYKAVLLLRLLREAGHDVRVIPTHAALEFVGRPTWEALSGHPATPEVFEDVEHVPHVSLGQGADLLVVAPATADLLSRAATGRADDLLTSTLLTVRCPVLLAPAMHTEMWDHPATRANVATLRQRGVHVLEPASGRLTGSDTGPGRLPEPAEIAAALEALLEPRGLADKQIVVSAGGTREPIDAVRYIGNRSSGKMGYAVAEAARDRGAIVTLVTSVTEIPPPVGVEVMPVETALEMRDAVLKASAAADVLIMAAAVADYHPARVAEGKIKRESETLTLDLVKTADILSEVKGDIIKVGFAAESEDLIARATDKLAKKNLDLIAVNDITAEDSGFGTDTNRVTLLFADGPAEALELADKQIVVSAGGTREPIDAVRYIGNRSSGKMGYAVAEAARDRGAIVTLVTSVTEIPPPVGVEVMPVETALEMRDAVLKASAAADVLIMAAAVADYHPARVAEGKIKRESETLTLDLVKTADILSEVKGDIIKVGFAAESEDLIARATDKLAKKNLDLIAVNDITAEDSGFGTDTNRVTLVGRSGEGPGDLPKEEVADVVFDRVIALLNR",
    "response#3": "MRILLGVAGGIAAYKAVLLLRLLREAGHDVRVIPTHAALEFVGRPTWEALSGHPATPEVFEDVEHVPHVSLGQGADLLVVAPATADLLSRAATGRADDLLTSTLLTVRCPVLLAPAMHTEMWDHPATRANVATLRQRGVHVLEPASGRLTGSDTGPGRLPEPAEIAAAALALVGRTPDLLARLAGRHVVVSAGGTREPIDPVRFLGNRSSGRQGLALARAARARGARVTLVAANVTLPDPDGVDVVHVESTAELRDAVHAAAKDADVVVMAAAVADFRPASTAGHKIKKRDDGVVAPIALEANPDVLAELAGELRRPGQVVVGFAAETGDDAGDVLEHGRHKARRKGADLLAVNAVGGGLGFGTADNEVTILDADGEPVEVARGSKHAVAGAVLDAVQRLLDG"
  },
  {
    "reference": "MFQNVAIVGATGAVGRIALQLLEERKFEAGDFKFLASARSAGSTLTFQDREYTIEELCPEAFEGVDLVLSSTPDDVAEKYLPAAVEAGAKVIDESGFWRMHPDVALVVPEINPEAALAAEGIIASPNCSTTQMVLALKPLYDVSRITRVIVSTYQAVSGAGLQGTADLLEGTRAALDETEYECQAFAHPIAFNAIPQIGSDKGGGYTSEELKMVYETRKILDDPQILVSPTCVRVPVANCHSESITVETEESISPEEARELFEAFPGLTVVDTLQDCEYPMPSTCTGSDETFIGRIRQDISHPNGLSFWCVSDNLRKGAATNAVQIAELLSAKQDCA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be designed to catalyze the following reaction L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.2. The designed protein should contain one or more Semialdehyde dehydrogenase NAD-binding domains that are essential for its function.3. The protein design should be able to enhance the efficiency of isoleucine biosynthetic process, threonine biosynthetic process, diaminopimelate biosynthetic process, lysine biosynthetic process via diaminopimelate. The designed protein sequence is ",
    "response#1": "LGGLFNQGWVEWLSAQTYQAASGGGAKNMRELVQQMRIVGENAGTLLDDPASAILEIDRNVVESLRSTMLPINCFGAPLAASLIPWIDTPMDNGQTREEWKGISETNKILGIEDNPIPVDGCCVRVGAMRCHSQAFTIKLTGDIPLDEIEQVIQEQNDWAHLVPNTKEETLKELTPARVTGTMDIPVGRVRKMNMGPEYLSAFSVGDQLLAF",
    "response#2": "LGGLFNQGWVEWLSAQTYQAASGGGAKNMRELVQQMRIVGENAGTLLDDPASAILEIDRNVVESLRSTMLPINCFGAPLAASLIPWIDTPMDNGQTREEWKGISETNKILGIEDNPIPVDGCCVRVGAMRCHSQAFTIKLTGDIPLDEIEQVIQEQNDWAHLVPNTKEETLKELTPARVTGTMDIPVGRVRKMNMGPEYLSAFSVGDQLLD",
    "response#3": "LKPIHDAVGISRINVATYQAVSGVGKSGVDELASQTARLLNGQSHESSVFTKQIGFNAIPHIGAFEENGYTNEEMKMVWETQKIFEDESILVNPTCVRIPVFYGHSEVVSVETRDPISVSKTRELLTEMPGIVLIDERTDGGYPTAVTEAAGADPVYVGRIRQDISTSNGLTLWIVSDNVRH"
  },
  {
    "reference": "MDPVVVLLLSLFFLLFLSLWRPSSGRGKLPPGPTPLPIIGNFFQVDMKDIRQSLTNFSKTYGPVYTLYVGSQPTVVLHGYEALKEALVDHGEEFSGRGRLPICEKVAKGQGIAFSHGNVWKATRHFTVKTLRNLGMGKGTIEDKVQEEAKWLVKELKKTNGSPCDPQFIMGCAPGNVICSIILQNRFDYEDKDFLNLIEKVNEAVKIISSPGIQVFNIFPILLDYCPGNHNIYFKNHTWLKSYLLEKIKEHEESLDVSNPRDFIDYFLIERNQENANQWMNYTLEHLAIMVTDLFFAGIETVSSTMRFALLLLMKYPHVTAKVQEEIDHVIGRHRSPCMQDRSHMPYTNAMVHEVQRYIDIGPNGLLHEVTCDTKFRNYFIPKGTAVLTSLTSVLHDSKEFPNPEMFDPGHFLDENGNFKKSDYFIPFSAGKRMCLGESLARMELFLFLTTILQNFKLKSLVDPKDINTTPICSSLSSVPPTFQMRFIPL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must bind to heme in order to perform its enzymatic function.2. The designed protein should have a high specificity for heme binding, with minimal off-target effects.3. For general function, the protein need meet that In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway; It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.4. The designed protein should have heme binding, oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen, aromatase activity, iron ion binding, monooxygenase activity within endoplasmic reticulum membrane, cytoplasm, intracellular membrane-bounded organelle to regulate epoxygenase P450 pathway, organic acid metabolic process.5. The protein should have a fast turnover rate for an organic molecule + O2 + reduced [NADPH--hemoprotein   reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein   reductase]. The designed protein sequence is ",
    "response#1": "MPQLDPTSWFSILFVMFTMFAVYLTRIYRICFKSFSETRPNANHNFWSWSSSTLLN",
    "response#2": "MLALCVQGWSPRVLAYLLLFCCLGTWAQSIRVPLENTVEGKSIQLPCPFTQNYNISWFKVENFERCIPLTSKADIPTELASHVSFSTKSPNSNSLIINKLTKTDEGIYWCKLNTIQRASLKGTYLRVRSCEDTSVPTTMAPKTTKITKPPTVTIQPTTTRSLPTQKVTTTTKSPNTRTTRSTHAMTQTTTSQVTKPTQTPTTTPTTTKTTKSTTSKPTMKPTRSPTTTPTTTKTTKSTTSKPTMKPTRSPTTTPTTTKTTKSTTSKPTMKPTRSPTTTPTTTKTTKSTTSKPTMKPTRSPTTTPTTTKTTKSTTSKPTMKPTRSPTTTPTTTKTTKSTTSKPTMKPTRSPTTPTTTKTTKSTTSKPTMKPTRSPTTTPTTTKTTKSTTSKPTMKPTRSPTTTPTTTKTTKSTTSKPTMKPTRSPTTTPTTTKTTKSTTSKPTMKPTRSPTTTPTTTKTTKSTTSKPTMKPTRSPTTTPTTTKTTKSTTSKPTMKPTRSPTTPTTTKTTKSTTSKPTMKPTRSPTTPTTTKTTKSTTSKPTMKPTRSPTTTPTTTKTTKSTTSKPTMKPTRSPTTTPTTTKTTKSTTSKPTMKPTRSPTTTPTTTKTTKSTTSKPTMKPTRSPTTTPTTTKTT",
    "response#3": "MCTYKLITLTLCLSSVLADTLCKRATESSQIPDYISCNYERCVLEKKCSTIDFECPFGCSCIYPNAVEKSDIPKCVPESDCPAEVSSPMVCGTDGKTYSSICELQVASCQHSMELSLAYKGACGESDDSCTKETCTNRFQPVCGSDGITYSSKCHLEVAACKAEKKITVAYEGTCETVGVSCTESACASQYKPVCGSDGKTYSNECSLKVASCKQNTELSPSYQGVCSSSDPCEAKKCESRYQPVCGSDNKTYSNECHLKVASCKQSTELSLAYQGPCSSSGSCTEETCINRYQPVCGSDGKTYSNECSLKVASCKQNTELSPSYQGVCSSSDPCEAKKCESRYQPVCGSDNKTYSNECSLKVASCKQNTELSPSYQGVCSSSDPCEAKKCESRYQPVCGSDNKTYSNECSLKVASCKQNTELSPSYQGVCSSSDPCEAKKCESRYQPVCGSDNKTYSNECSLKVASCKQNTELSPSYQGVCSSSDPCEAKKCESRYQPVCGSDNKTYSNECSLKVASCKQNTELSPSYQGVCSSSDPCEAKKCESRYQPVCGSDNKTYSNECSLKVASCKQNTELSPSYQGVCSSSDPCEAKKCESRYQPVCGSDNKTYSNECSLKVASCKQNTELSPSYQGVCSSSDPCE"
  },
  {
    "reference": "MVETGHHHQHPPAPAAAGHPPVPSMAMARNMGTTWPPAEQLHHLQYCIHSNPSWHETVVLAFQHYIVMLGTTVLIANTLVSPMGGDPGDKARVIQTILFMSGINTLLQTLIGTRLPTVMGVSFAYVLPVLSIIRDYNNGQFDSEKQRFRHTMRTVQGSLIISSFVNIIIGYGQAWGNLIRIFSPIIVVPVVSVVSLGLFLRGFPLLANCVEIGLPMLILLIITQQYLKHAFSRISMILERYALLVCLAIIWAFAAILTVSGAYNNVSTATKQSCRTDRAFLMSSAPWIRIPYPFQWGTPIFKASHVFGMFGAAIVASAESTGVFFAASRLAGATAPPAHVVSRSIGLQGIGVLLEGIFGSITGNTASVENVGLLGLTRIGSRRVVQVSTFFMIFFSIFGKFGAFFASIPLPIFAGVYCILLGIVVAVGISFIQFTDTNSMRNMYVIGVSLFLSLSIAQYFLANTSRAGYGPVRTAGGWFNDILNTIFASAPLVATILATILDNTLEARHASDDARGIPWWKPFQHRNGDGRNDEFYSMPLRINELMPTRFL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have adenine transmembrane transporter activity, guanine transmembrane transporter activity, solute:cation symporter activity, uracil transmembrane transporter activity within plasma membrane, plasmodesma to regulate hypoxanthine transport, adenine import across plasma membrane, uracil import across plasma membrane.2. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "NVAVMGSACLCAGFGSSIFSGAVPEVMVKFHVCRTVALLGISLYVLGFASGPVVWAPMCELFGRRRPMIIAVFIFCIFHIAVATAKDIQTVMICRFFCGFFGSSPITTVAGSFSDMFSARTRGLVIAVYSAIIFNGPLMSPIVGGFIGKSYLGWRWTSYITAIMGFTAFTSMIIFHRETYTRTITEIRASKVRVLTGNYCLHAKSEEEPLEFSYFFHKYFTFPLRLLIFEPILLVVSTYTAFVYGILYGLLEAYPVIFGESRKWRLGVESLPYLAIFVGVCIGCSSVALFQPYYFKKMDENKGRPVPEARLPSMMIGCIVFPIGIFWLAWTGNYPWIHWIVPTLAGSFIGFGIITIFQQTINYIIDCYSGCSASAIAANTLLRSSFGAAFPLFTTQMFNNLGIGWAGSLVGFVAVGLIPVPFMLFLYGPKL",
    "response#2": "NVAVMGSACLCAGFGSSIFSGAVPEVMVKFHVCRTVALLGISLYVLGFASGPVVWAPMCELFGRRRPMIIAVFIFCIFHIAVATAKDIQTVMICRFFCGFFGSSPITTVAGSFSDMFSARTRGLVIAVYSAIIFNGPLMSPIVGGFIGKSYLGWRWTSYITAIMGFTAFTSMIIFHRETYTRTITEIRASKVRVLTGNYCLHAKSEEEPLEFSYFFHKYFTFPLRLLIFEPILLVVSTYTAFVYGILYGLLEAYPVIFGESRKWRLGVESLPYLAIFVGVCIGCSSVALFQPYYFKKMDENKGRPVPEARLPSMMIGCIVFPIGIFWLAWTGNYPWIHWIVPTLAGSFIGFGIITIFQQTINYIIDCYSGCSASAIAANTLLRSSFGAAFPLFTTQMFNNLGIGWAGSLVGFVAVGLIPVPFMLFLYGPKL",
    "response#3": "PGQTLALVGPSGSGKSTIIRLLFRFYDVQGGCIRIDGQDISQVKQASLRAHIGVVPQDTVLFNDTIANNIRYGRILATDQEVQEAARAADIHDRILSFPDGYNTQVGERGLKLSGGEKQRVAIARTILKGPHIILLDEATSALDTETERNIQASLAV"
  },
  {
    "reference": "MDIRCAALSLPMLRTIMVLLMASSAWSVWPSDPMEVVQDWDNQLEASMHSLFPEIKEAVAPMTGIPDSSAVVGRPFKIHIPTEFLASSGETIKIFEVGKEILPSWLHWEANFLQGLPLDGDKGVYDISVASMRLAPNGSYVTQTADVFAVEVHPEDHNEPQSVRVAGQETAEATPFLCGTDEPVTILTVILDADLTKMTPKQRVDLLNRMRDFSEVELYHMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCGMDQNTVPNISSVEVPAKEGTMSAQLGYPVVGWHIANKKPQMPKRIRRQIYATPTPVTAIGPPTTAIHEPPERIVPTPTSPAIAPPTDTTAPPVREPIPLPGKPTVTIRTRGAIIHTPTLGPIHPTRIIETTSIVRPTITRHIFVEPTAAVTPPSTTTKRPRTTMKPPTPPTTDSSTTTTKKPTKKPRPRPPKPLATTKAPSTKVETTSPSRTRPSTSGVPNTDPELKNHIDKVVAWVGTYFEVKIPPDTFYDKEDGTTDNLQLSLAPRQKAGLGEKMWVMLNSTSQVMYGMPDYTHIGDHEYYLRAADKAGRTAVDALEIQVRNLFQKQPSTVKFHAKFHGDHNAVINDINKKILLVKKLAFAFGDRNSSSITLHNITKGSVVVDWTNNTFPVEPCPVEQVEGVGKKIYDERGSPRQHFVNAVEPEFKLLNISLSFSGSCKHKKFRYIPMRAEEPIPTAVAPTVAVDRNLEKRSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein contains novel Peptidase S72 domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "RNNSGVYHITNDCPNSSIVYETENHILHLPGCVPCVRSGNVSRCWVSLTPTLAAPQLNAPLQPLRKHVDLMVGAATMCSALYIGDLCGGLFLIGQAFTFQPRRHLTTQDCNCSIYSGHITGHRMAWDMMMNWSPTTTLVLAQIMRIPQVVFDLLSGGHWGILVGVAYFSMQGHWAKVILVLFLFAGVH",
    "response#2": "GQAHDITIKRDKIHVKSVEYQKHGDVGVFTINKFQNSTSGELKSAIIKAHKDGIRKIVLDLRNNPGGLLDEAVKMANIFIDKNETVVQLEKGKHKEAIKASNDASKEAKDMDVSILVNKGSASASEVFTGAMKDYNKAKVYGSKTFGKGIVQTTREFEDGSLLKFTNMKWLTPKSHYIHGKGITPDKKIC",
    "response#3": "RNNSGVYHITNDCPNSSIVYETENHILHLPGCVPCVRSGNVSRCWVSLTPTLAAPQLNAPLQPLRKHVDLMVGAATMCSALYIGDLCGGLFLIGQAFTFQPRRHLTTQDCNCSIYSGHITGHRMAWDMMMNWSPTTTLVLAQIMRIPQVVFDLLSGGHWGILVGVAYFSMQGHWAKVILVLFLFAGVH"
  },
  {
    "reference": "MNFQNGMIPPQYGGGNNPYSQPPYAMPPDVQGMQQHSMHMAPPMNAPEMGPMGESEYRHVSSVLGGQPGNAVTSTAFDSVEELLWVGTRAGYVSSYYGPSLQKYTAFRVHSSNPVIQLMPSHQGIFTLTQENVRCYARFGRGTADFMSEDFHNMQCMLHTVNNSILVGGHQQSVFELDIDRLALIKKYQIEDPGVAIMKSNNRFTIFGDTSGQITIRDPKTLKIENQFQAHSFTLSDFDAYENQIVTCGFSNVAPMRGGNQIDRFLMVYDVRMMRIVSTIQCAIEPYYLKFVPSYTSRVFVVAHSGQYQIIDTTGAMMSAPLLSHQIQAATGPISAFDVSSSHQSVSFGDEAGCCHLYSNAPNVEGSHAAFNPYSQETQFADVTERLPHMNIDDPMANYASVPMPYPGQGSLFSDWPPAYTQRVYRAPAPIDPAILNNVKMTGNLGYGKMPEGVARPFPYQLKSEPGYRGGKQRGGGTHSTRGGRSKSHVPASPIGREDDPFLSVPKQYRKVEIKYSKLGVEDFDFRHYNKTHFAGLETHIPNAYCNSMLQVLYFIEPLRATLLNHLCQQEFCLACELGFLFHMLDVSKGMTCQASNFLRAFRTIPEASALGLNLGDDDEASKKNLARIIQNWSRFVMQQIHTEIGRNPKDSTPSSETSPDVQTVVQKLFGTSTATNLSCSKCGHLSSRESETFMFNMDYPEAPVDVPHFAFPKVPFSEVIARSMNAAQSQQSFCQKCDGYQLHTQQRLLKNVPDVIGLNCQVDNDTT",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must be optimized for nucleic acid binding, metal ion binding.2. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "VVESVRFSFMTEQDVRKHSFLKVTSPILHDNVGNPFPGGLYDLKLGPKDDKQACNSCGQLKLACPGHCGHIELVFPIYHPLLFNLLFNFLQRACFFCHHFMAKPEDVERAVSQLKLIIKGDIVSAKQLESNTPTKSKSSDESCESVVTTDSSEECEDSDVEDQRWTSLQFAEVTAVLKNFMRLSSKSCSRCKGINPKLEKPMFGWVRMRAMKDSDVGANVIRGLKLKKSTSSVENPDGFDDSGIDALSEVEDGDKETREKSTEVAAEFEEHNSKRDLLPSEVRNILKHLWQNEHEFCSFIGDLWQSGSEKIDYSMFFLESVLVPPTKFRPPTTGGDSVMEHPQTVGLNKVIESNNILGNACTNKLDQSKVIFRWRNLQESVNVLFDSKTATVQSQRDSSGICQLLEKKEGLFRQA",
    "response#2": "VVESVRFSFMTEQDVRKHSFLKVTSPILHDNVGNPFPGGLYDLKLGPKDDKQACNSCGQLKLACPGHCGHIELVFPIYHPLLFNLLFNFLQRACFFCHHFMAKPEDVERAVSQLKLIIKGDIVSAKQLESNTPTKSKSSDESCESVVTTDSSEECEDSDVEDQRWTSLQFAEVTAVLKNFMRLSSKSCSRCKGINPKLEKPMFGWVRMRAMKDSDVGANVIRGLKLKKSTSSVENPDGFDDSGIDALSEVEDGDKETREKSTEVAAEFEEHNSKRDLLPSEVRNILKHLWQNEHEFCSFIGDLWQSGSEKIDYSMFFLESVLVPPTKFRPPTTGGDSVMEHPQTVGLNKVIESNNILGNACTNKLDQSKVIFRWRNLQESVNVLFDSKTATVQSQRDSSGICQLLEKKEGLFRQAL",
    "response#3": "SGDNPWEFSLTYSNKEKLNEIGETLKAIHGENGLRRFGTLPKIDQFVVCQVGKNFFRARVLKTFQNGRVKVSKSDFNEDVVLQLPNVYELPREVASIPCQSVRCCLQ"
  },
  {
    "reference": "MKPVKIGVAGPVGSGKTALIDRLAKAMRDRYNLAVITNDVYTYEDAEFLVRSGALPPERIAGVRTGGCPHTAIREDPAANQEAVEAMLARFLDLDILFLESGGDNLAASFSPELVDVWIYVIDVAAGDKIPRKGGPGIEKSHLLVINKIDLAPLVGADLGVMERDTRLKRGSRPWVFTNLKTGEGLDSVVEWIRREVLFEGD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein can be expressed in cytoplasm and perform GTPase activity, GTP binding, nickel cation binding without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "ADVALVGFPSAGKSSLVSVLSAAKPKIADYPFTTLVPNLGVAQAGDRPPYTVADVPGLIPGASEGRGLGLEFLRHIERCSVIVHVLDCATLEPGRDPMTDLDVIEAELAAYSADLSDRPRLVVLNKIDVPDAAELAELVAPELRARDLAVFAVSTATRRGVHALSLALADLVK",
    "response#2": "PVVTIMGHVDHGKTSLLDSIRATKVAAGEAGGITQHIGAYHVDVEHEGQMQQVVFLDTPGHEAFTAMRARGARVTDVAVLVVAADDGVQPQTIEAISHAKAAEVPLIVAINKIDKEEANPDRVKQELMEHGLVPEEWGGDAIMVPVSAIQKQNLDTLLEME",
    "response#3": "ADVALVGFPSAGKSSLVSVLSAAKPKIADYPFTTLVPNLGVAQAGDRPPYTVADVPGLIPGASEGRGLGLEFLRHIERCSVIVHVLDCATLEPGRDPMTDLDVIEAELAAYSADLSDRPRLVVLNKIDVPDAAELAELVAPELRARDLAVFAVSTATRRGVHALSLALADLVA"
  },
  {
    "reference": "MTSLPSSVIKWRFFRRQMPSDVVFSLCLLCFASCLAGKITVLADSDKSVLLRFKKTVSDPGSILASWVEESEDYCSWFGVSCDSSSRVMALNISGSGSSEISRNRFTCGDIGKFPLYGFGVRRDCTGNHGALAGNLPSVIMSLTGLRVLSLPFNSFSGEIPVGIWGMEKLEVLDLEGNLMTGSLPDQFTGLRNLRVMNLGFNRVSGEIPNSLQNLTKLEILNLGGNKLNGTVPGFVGRFRVLHLPLNWLQGSLPKDIGDSCGKLEHLDLSGNFLTGRIPESLGKCAGLRSLLLYMNTLEETIPLEFGSLQKLEVLDVSRNTLSGPLPVELGNCSSLSVLVLSNLYNVYEDINSVRGEADLPPGADLTSMTEDFNFYQGGIPEEITRLPKLKILWVPRATLEGRFPGDWGSCQNLEMVNLGQNFFKGEIPVGLSKCKNLRLLDLSSNRLTGELLKEISVPCMSVFDVGGNSLSGVIPDFLNNTTSHCPPVVYFDRFSIESYSDPSSVYLSFFTEKAQVGTSLIDLGSDGGPAVFHNFADNNFTGTLKSIPLAQERLGKRVSYIFSAGGNRLYGQFPGNLFDNCDELKAVYVNVSFNKLSGRIPQGLNNMCTSLKILDASVNQIFGPIPTSLGDLASLVALNLSWNQLQGQIPGSLGKKMAALTYLSIANNNLTGQIPQSFGQLHSLDVLDLSSNHLSGGIPHDFVNLKNLTVLLLNNNNLSGPIPSGFATFAVFNVSSNNLSGPVPSTNGLTKCSTVSGNPYLRPCHVF",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a binding site that is specific to the target ATP ligand.2. For general function, the protein need meet that Together with RPK1, required for pattern formation along the radial axis (e.g; the apical embryonic domain cell types that generate cotyledon primordia), and the apical-basal axis (e.g3. The designed protein should have protein serine kinase activity, ATP binding, protein serine/threonine kinase activity to facilitate pollen germination, protein phosphorylation.4. The protein with the reaction ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-   threonyl-[protein], the Proton acceptor must be optimized to ensure efficient catalysis.5. The Extracellular topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.6. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "YELGRTLGEGTFAKVKFARNVENGDNVAIKVIDKEKVLKNKMIAQIKREISTMKLIKHPNVIRMFEVMASKTKIYFVLEFVTGGELFDKISSNGRLKEDEARKYFQQLINAVDYCHSRGVYHRDLKPENLLLDANGALKVSDFGLSALPQQVREDGLLHTTCGTPNYVAPEVINNKGYDGAKADLWSCGVILFVLMAGYLPFEDSNLTSLYKKIFKAEFTCPPWFSASAKKLIKRILDPNPATRITFAEVIENEWWKKGRLLGMGSFGHVYLGFNSESGEMCAMKEVTLCSDDPKSRESAQQLGQEISVLSRLRHQNIVQYYGSETVDDKLYIYLEYVSGGSIYKLLQEYGQFGENAIRNYTQQILSGLAYLHAKNTVHRDIKGANILVDPHGRVKVADFGMAKHITAQSGPLSFKGSPYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQYEGVPAMFKIGNSKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFFQHSNDRSLKKGRLLGMGSFGHVYLGFNSESGEMCAMKEVTLCSDDPKSRESAQQLGQEISVLSRLRHQNIVQYYGSETVDDKLYIYLEYVSGGSIYKLLQEYGQFGENAIRNYTQQILSGLAYLHAKNTVHRDIKGANILVDPHGRVKVADFGMAKHITAQSGPLSFKGSPYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQYEGVPAMFKIGNSKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAF",
    "response#2": "DVLGRGAFKTVYKAFDEVDGIEVAWNLVSIEDVMQMPGQLERLYSEVHLLKALKHENIIKLFYSWVDEKNKTINMITELFTSGSLRVYRKKHRKVDPKAIKNWARQILKGLNYLHSQNPPVIHRDLKCDNIFVNGNTGEVKIGDLGLATVLQQPTARSVIGTPEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYNECRNQAQIYKKVTSNIKPQSLGKVDDPQVRQFIEKCLLPASSRPTALELSKDPFL",
    "response#3": "IRYDDVLGRGAFKTVYKAFDEVDGIEVAWNLVSIEDVMQMPGQLERLYSEVHLLKALKHENIIKLFYSWVDEKNKTINMITELFTSGSLRVYRKKHRKVDPKAIKNWARQILKGLNYLHSQNPPVIHRDLKCDNIFVNGNTGEVKIGDLGLATVLQQPTARSVIGTPEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYNECRNQAQIYKKVTSNIKPQSLGKVDDPQVRQFIEKCLLPASSRPTALELSKDPFFAQ"
  },
  {
    "reference": "MWNEFKKFALKGNVMDLAVGVVIGGAFGKIVSSLVSDVIMPLVGLLLGGVNFTGLSFTFGKAVVKYGAFIQTVVDFLIIAFSIFLFIKLFNKLTFKKEEEKKEEVPEPTKEEVLLGEIRDLLKQQNASKDRA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that May participate in the regulation of osmotic pressure changes within the cell.; Channel that opens in response to stretch forces in the membrane lipid bilayer2. The protein can be targeted to plasma membrane and perform mechanosensitive ion channel activity.3. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "IFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYL",
    "response#2": "IFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYR",
    "response#3": "GMVY"
  },
  {
    "reference": "MKVAVVGATGLVGTKMLEVLAERNFPVTELIPVASEKSVGKKLLFKNREWSVVSAADAIKAKPAIAIFSAGGGTSLEWAPKFAAAGITVIDNSSAWRMDPTKKLVVPELNADALTKEDKIIANPNCSTIQMVVALNPLHLKYHIKRIVVSTYQSVTGTGVKAVNQLMNERKGVQGEMAYKYPIDLNVIPQIDVFLDNGYTKEEMKMVNETKKIMRDDSIRVTATTVRIPVVGGHSEAVNVEFEKDFDLAEVKSLLEKAPGVIVVDNPAKHEYPMPKDAHNRDEVFVGRIRRDETQPNTLNMWIVSDNLRKGAATNAVQIAEYLVGKGLV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein with NADP binding, NAD binding, aspartate-semialdehyde dehydrogenase activity is needed to enhance isoleucine biosynthetic process, diaminopimelate biosynthetic process in a specific context.2. The designed protein should have a binding site that is specific to the target phosphate ligand.3. The Acyl-thioester intermediate of the protein must be engineered to enable effective catalysis of L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH. The designed protein sequence is ",
    "response#1": "VAIVGATGQVGTVMREILAERNFPIGELRLFATSRSAGTTVEFAGQSVVIEDIETADPAGIDIALFSAGATGSRAHAPRFAEAGALVIDNSSAWRMDPEVPLIVSEVNPHAIAEAGKGIIANPNCTTMAAMPVLKPLHDRARIKRVVVSTYQAVSGAGKEGVDELWNQTKGVYVPGQEVEPEKFQKQIAFNVIPHIDVFMEDGQTKEEWKMVSETKKIVDPSIKVTATCVRVPVFVGHSEAVNLEFEDFLDEDEARDILREAPGIMVVDKREDGGYVTPVECVGDFATFVSRIRQDPTIENGLNLWVVSDNLRKGAALNAVQIAELVAAKL",
    "response#2": "MLAPLAKAFGLERVVVSTYQAVTGTGQKAVDRLLAEREGRFAGPSPYPHPIDLNCLPQCDVFEAEGYTKEEWKIIRESRKILERPDLAITATAVRVPVLGGHSESVNVVLCREASLEEVRRVLEEAPGLTLQDDPASSYYPMPRYVGGQDGVFVGRIRRDTSHPRGFNFWVVADNLRDN",
    "response#3": "LAPIKAKYGLTRVIVSTYQAASGAGQSAWEELVAETTAHNAGQEMHANILPVKNAEHHYPLAYNLLPQIDVFEDDGYTHEEWKMIHETKKIMFGDKNSKAVKVTGTAVRVPVPIGHGEAVYFETEAGTGATSGDIQSILSKAPGVVLEDNPKEQLYPQPINAEGKRETFVGRVRPDLENDDSFHMWVVSDNLLG"
  },
  {
    "reference": "MAAGGRGLIRALHSSPCPTWKRAQSGANGRLKPEYDAVVIGAGHNGLVAAAYLQRLGVNTAVFERRHVIGGAAVTEEIIPGFKFSRASYLLSLLRPQICTDLELKKHGLKLHLRDPYSFTPMLEEGTLNRLPRSLLLGTDMAANQKEISQFSRKDAQAFPRYEEFMKRLVLAIDPLLDAAPVDTTAFQHGSLLQRLRALSTLKPLLKAGRTLGAQLPQYYEVLTAPISKVLDQRFESEPLKATLATDAVIGAMTSPHTPGSGYVLLHHVMGSLEGTQGAWSYVQGGMGALSDAIASSAATRGASIFTEKTVAKVQVNSEGRAQGVTLQDGEEVRSRVVLSCASPQVTFLELTPQEWLPGAFVKRISQLDTQSPVTKINVAVDRLPNFQAAPNAPGDQPQGHHQCSIHLNCEDTLLLHQAFEDAKGGLPSQKPMIELCIPSSLDPTLAPPGCHVVSLFTQYTPYTLAGGKVWNEQEKNTYADKVFDCIEAYAPGFKRSVLARDILTPPDLERIFRLPGGNIFHGAMSLDQLYFARPVPQHSDYRCPVQGLYLCGSGAHPGGGVMGAAGRNAAHVVFRDLKNM",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have oxidoreductase activity within mitochondrial matrix to regulate organonitrogen compound metabolic process, mitochondrion organization.2. The protein must exhibit the following characteristics: Probable oxidoreductase that may play a role as regulator of mitochondrial function.. The designed protein sequence is ",
    "response#1": "GDKVLVVWTQPSTAVAVKDFAESIGAIVGSQSLVTLENIERLQLSSHSPSSYDWVLSGLLPDSALLHGPDTLAEMAKVLKPGGRLLLEEPVAGLEVSGVRTSGKLLSALKLSGLVSVTEVKSEALGPKAALALRERTGFQGNAAFRVRMSASKPNFEIGSD",
    "response#2": "GDKVLVVWTQPSTAVAVKDFAESIGAIVGSQSLVTLENIERLQLSSHSPSSYDWVLSGLLPDSALLHGPDTLAEMAKVLKPGGRLLLEEPVAGLEVSGVRTSGKLLSALKLSGLVSVTEVKSEALGPKAALALRERTGFQGNAAFRVRMSASKPNFEIGSV",
    "response#3": "GDKVLVVWTQPSTAVAVKDFAESIGAIVGSQSLVTLENIERLQLSSHSPSSYDWVLSGLLPDSALLHGPDTLAEMAKVLKPGGRLLLEEPVAGLEVSGVRTSGKLLSALKLSGLVSVTEVKSEALGPKAALALRERTGFQGNAAFRVRMSASKPNFEIGSC"
  },
  {
    "reference": "MCSSLAGPDCCGGLDNIDFRQADLCVMNWLLGYVDPSDPYFVAAVLTIAFNPLFWNVASLEGWKRAGGPWSCGRGFAEAELRTSCDHLEGARRVARWEQKTRKLSRAFGSPYLACYALGGAIVLLNVLRSHCFTQAMLSQPRMHSLDNPTAYLVGLALLAVGGVLVLSSFFALGFTGTFLGDYFGILKEARVTTFPFNILDNPMYWGSTANYLGWAVMHASPAGLGLTAVVALVYVIAILYEEPFTAEIYRRKSSQLRKRS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure2. The protein should have Helical domains to ensure proper insertion into the membrane.3. The designed protein should have a high specificity for S-adenosyl-L-methionine binding, with minimal off-target effects.4. The Cytoplasmic, Lumenal topological domain of the protein should be designed to be flexible or rigid.5. A protein that can perform phosphatidyl-N-dimethylethanolamine N-methyltransferase activity, phosphatidylethanolamine N-methyltransferase activity, phosphatidyl-N-methylethanolamine N-methyltransferase activity in mitochondrial membrane, endoplasmic reticulum membrane to support methylation, phosphatidylcholine biosynthetic process.6. The protein have enhanced a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + S-adenosyl-L-   methionine = 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine +   H(+) + S-adenosyl-L-homocysteine.7. A protein that is capable of catalyzing reactions within the phosphatidylcholine biosynthesis, Phospholipid metabolism pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MTAPAPAIIHPAPPTGTAPAVVAPRPAPSTSAAPRPAGSTTATPGGPAASTASLPAPAPAGPVAAATASRAGPAGTTTAGAGAPGTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPSTTAAGPGAPS",
    "response#2": "MVCLWLCLLASVLGLAPCSAQTPGVWHRGPQSAPSPSRPGPGQPLPRVLVPVVLVNTRDFSAARQTGLLGALLPQDQALHLHLARRPGPRAGGPGPTLLLPEASRLLGPAEPPLQRLLLRLARLGSPAHVLEYGAGRATLALAAALPRGCRFLAVEHHASFHARLRRELARNSLPANVTVLHRDASKWERELGSGGDPGLLGRWPRCRLLAFLGHPPCGDCVSAVLRALAPPGLAVLLLGPRRDPPQLLAPLSQKYHWQVSLLHLATPGQPTWHRQDVYLLSPGAHLP",
    "response#3": "MEPNHHNPTTPSTPGTNGSEPTAPPTGSAATRLRPVPGSISGSGSSGGGGGGG"
  },
  {
    "reference": "MQGQTTSVSFDGREIRLTTGRYAPQAGGSVLIECGDTAVLVTATQGQGREGADFLPLSCDYEERLYAAGRIPGSFMRREGRPPERATLISRLIDRPLRPLFPNWMRDDIQVVATCLSLDERVPADILAVTGSSMATLLAGIPFYGPMAAVRVGLLGDDFVLNPSFREIERGDLDLVVAGTPDGVVMVEAGSNQLTEQDVIEAIDFGYEAVNELIKAQESILKDSGLTQIKPEKPDLDETVPSYLEKNCTKPISALLKEFDLSKEDRDLKLDEIKTNCAEKIDSLKDDNAVKKSITTNTKLLGISFKALTKKLMREQIIKDGKRVDGRALDEVREISAEAGILPKRVHGSGLFQRGLTQVLSTATLGTPSDAQEMDDLNPSPDKTYIHHYNFPPYSVGETRPMRTPGRREVGHGALAERAIIPVLPPKESFPYVLRVVSEVLSSNGSTSMGSVCGSTIALLDAGVPLKAPVSGAAMGLIKEGEEVRILTDIQGIEDFLGDMDFKVAGTEKGITALQMDMKMTGLPIKIIGEAINQAKPARTHILEKMVQAIDKPRETLSPHAPRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIEDGGIVTIASHDGVAAEEAQKIIEGLTRKVHEGEVFTGSITRIIPIGAFVEILPGKEGMIHISQLSEARVEKVEDVVKVGDEVTVRVREIDNRGRINLTLRGIPQNGDMQYYPQPTPTPVAPLM",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a low Km value for phosphate + RNA(n+1) = a ribonucleoside 5'-diphosphate +   RNA(n).2. The protein should be able to bind Mg(2+) ligand in a variety of conditions3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.4. The designed protein should contain one or more S1 motif domains that are essential for its function.5. The protein must exhibit the following characteristics: Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'- direction.; Involved in mRNA degradation.6. A protein that contributes to mRNA catabolic process by functioning in cytoplasm with polyribonucleotide nucleotidyltransferase activity, magnesium ion binding. The designed protein sequence is ",
    "response#1": "LGAFISDACKARLKELAYTALAKEERAERTKEIYGETLKSAVEHFSAAFSSEDLAADPSKALCTNEHIIEEEIHSVEKDVMRRMILDDAKRLDGRV",
    "response#2": "EIRQIEVEVDILSRTHGSALFTRGSTQALVVTALGTTQDEQIVDDIEGDRREHFMLHYNFPSFAVGDASAIRAPGRREIGHGKLAWKAIHPVLPDKSEFPYTIRVVSEIMESDGSSSMATVCGTSLALMDTGVPFLGPIAGVNVGLVDGELIINPDVQQREVSVLDLQVAGHFDAVNMVEAGAKEVAEDKMLEAIMFGHALGAFISDACKARLKELAYTALAKEERAERTKEIYGETLKSAVEHFSAAFSSEDLAADPSKALCTNEHIIEEEIHSVEKDVMRRMILDDAKRLDGRKSVIDTVGADLSKAYKITAKGERYAAIGAAKDKAKAALLGTDEAPGVMTPEVFKTVFKEAEASVVRGDILKTGQRIDGRDELTETVRVLCETRLKELAYTPLKKEERADQTAAIYHEIIESTIEHFKATCSSEEFSTDPAKALCINPHIIDEQIHMVEKRVMRHMILDDAKRLDGR",
    "response#3": "LENYIKEKATEQIKEILSQYDLDKNQRDEKLDEIKDSLAESITELPEEEAVRAMVESESMLLGNAFKSVTKKLMRQQIVAEGIRVDGRKFEHAPKLTSIKVPVDCIGMIIGKGGETIRSITEETGAEINIDDDGTITIACSTSEGTNAALATIKNLTGF"
  },
  {
    "reference": "MLSTKTIEIVKSTAPLLAETGPKLTAHFYDRMFARHPELNDIFNMSNQFSGAQREALFNAVHGYAANIDNLPALLPVIEKIAQKHVSFNITPEMYAIVGENLLATIDELYSPGQEVLDAWAEAYGVLAHVFVTREEEIYQQSETSQGGWRGTRPFTLVSKTPESEVITSFVFAPQDGLPVAHYTPGQYIGIYLTPEQFANREIRQYSLSCAPNGKQYRISVKREAQGTVSNYLHDHLNVGDVVDLAPPSGDFFFQSDITTPVALISGGVGLTPMLAMLESLSGQHHADIQWLHAAEHGEQHAFGQHIDTLVEQHGNAQRNVWYREPKDTDQRGQHYDHQGMMDISKVSALTEDSQRHFYLCGPVGFMQSIAKQLTDSGITKDRIFYECFGPHKVLD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design must be able to perform heme binding, FAD binding, metal ion binding, nitric oxide dioxygenase activity, oxygen binding in order to contribute to response to toxic substance, response to nitrosative stress.2. The protein must exhibit the following characteristics: Therefore, plays a central role in the inducible response to nitrosative stress.; Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds.3. The Charge relay system of the protein must be engineered to enable effective catalysis of NADPH + 2 nitric oxide + 2 O2 = H(+) + NADP(+) + 2 nitrate.4. The designed protein must have at least one FAD-binding FR-type, Globin family profile domain.5. The protein design should incorporate a binding pocket that specifically recognizes heme b to enhance enzymatic efficiency.6. The FAD, heme b, NADP(+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MLSSSTIEIVKSTAPLIAETGPKLTAHFYDRMFAHHPELKDIFNLTHQDSGSQREALFNAIHGYAANIDNIEVLLPVVEKIAQKHTSFNITAEQYQIVGSHLLATIDELFSPGQPVLDAWGEAYGLLAQVFIDREQHQAAPQDSGGWQGTRAFRLREKIPQSALITSFEFEPVDGLPVADYQPGQYLGIWLKPEGFAHQEIRQYSLTRKPNGKTYRIAVKREAGGQVSEWLHDEAKPGDIVHLAAPAGDFFMQTEADTPVVLISAGVGQTPMLAMLDHLAKTQHRAQVNWFHAALDGQVHAFADEVSQLGASLNDFYQHVWYQQPQATDRGRFHSEGLMDLTAQEGKFSDPAMEFYLCGPVGFMQFAAKQLVELGVAQERVHYEVFGPHQDL",
    "response#2": "MLDNQTIAIVKSTIPLLAETGPKLTAHFYDRMFTHNPELKEIFNMSNQRNGDQREALFNAICAYATNIENLSALLPAVERIAQKHASFNIQADQYQIVGQHLLATLDEMFSPGQEVLDAWGKAYGVLANVFIQLQDEAERAYQAEREAAGWQGTRPFIIKEKRQESELITSFVLAPLDGNPVLRFKPGQYLSIKLVHPALEHQEIRQYSLSDAPNGRDYRISVKREPRGQASNLLHDHLHIGDTVDIMPPAGDFFLQAPDTQPVALISGGVGITPMMSMLESVAAEFQPGQYVTVRQGTRPFIIKEKRQESELITSFVLAPLDGNPVLRFKPGQYLSIKLVHPALEHQEIRQYSLSDAPNGRDYRISVKREPRGQASNLLHDHLHIGDTVDIMPPAGDFFLQAPDTQPVYALISGGVGLTPMQAMLDTLAKQNYSQPVSYLHACAHEGLHSFKDHVNGLKGALDLSVYTWYERAHELADSEFEGYMQLDDIKANLPLSNGEFYLCGPVGFMMFMPFMQAVYDGLAEAGVAEDRVHFEFFGPKGSLEATA",
    "response#3": "MLNQQTIAVIQSTVPVLKEYGTAITGRFYERMFEAHPELLNLFNHANQKQGRQREALANAVYAAALHIDKLEQILPAVRHIAQKHRSLGVKPEQYPIVGRHLLAAIKEVLGDAATDEILEAWADAYGVIADVFIEQDYEQQLQQHGTGWRTFVVREKRPESSVITSFVFEPADGGPVANFEPGQYISIAVNVPALGLQQIRQYSLSDMPNGRSYRISVKRESGGAYPPGYVSCLLHDHVNVGDEVRLAAPYGSFHINTCERDFQPGQYLAVYLISAGVGLTPLLSMLNHLLADGPDMDIRWLHACEHGAHHAFRDEIRAKSRQHGKLESRIWYREPGEQDVKGEDYDFSGTLALEQVTDLMPADAHYYFCGPVPFMKAVNGLLRAWGVPAARIHYEVFGPHKVLE"
  },
  {
    "reference": "MFSFFKQIFKSLKKFFFLLFGIIFVLFSIIFLETSILQLSNNLVNTYTALVQKTNSSDIVAPAIFKESSPVYKTELKEEKRHFSKIKLTEKKINFIWPYQESDFGSDSEKKDSTTKSSDNNPRKGDVNDKDKLFLARKRGILKAYGEANIAEKRIYKGLAVSFQNTYSFTGTDQESNNLNQNTVSDPQNLIYDKEGNLLGYFVDGLILDGIPLRAGIARFPGDKGKGEDKKTTKKKSEIKQASSATTVLQPLAAQAKMTDAKETTNNEEPKKDSNVEEQYTTNNKDKVWFKSDETQAGSSSGESETSKLSTSYLFTGGQEAANWFPNLYANVPIVLPISPGSQFWLETNPFKEIIEVFQKEKEEKEKQSFSLTFTLDTSKLSHLDKEEFDWLEKQAETISSGSSFGDYNLKKKINSLKSFELNINKDWLKNKVKAEKETILDSLPGFSNSDKNTIFSTQSGDAKSGTQSNPSSLIALRSSVSFKPQLQQTNVALAQQQQDKQESSADDGVKDPTFSDVQTEFDKIGTENHTPQKNLNNVYAALLHQWKSIFQEDLVKKVTALLEKYRDAFLKAKALKELEFSRQNLAIATNVSSEESASFLVSNKDSQKYNDLSIIEGINFKSWLAKEKSNPLDMVYGGKSNSEGFLEKVEYEFKPTQTDEKKKAAAKTTQGTTDSLTQLADASSSSSSSSTGDTKSTSTKFQIYPKLANILAQAQLPEASSIPDTLTNAIKQWSTLDKKGFEALDDTGKSKAANNYVALLSYFTPEF",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein localizes to the plasma membrane.2. The protein should have Helical domains to ensure proper insertion into the membrane.3. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "MCVMMPVSSWGAYIITLIGGLLATYSITEYTPIGAFVAMSSMNFYAIFSIIMVFFVAYFSFDIASMVRHEKLALKNTEDQLEEETGTKGQVRNLILPILVLIIATVSMMIYTGAEALAADGKVFSVLGTFENTVVGTSLVVGGFCSIIISTLLIILDRQVSVPEYVRSWIVGIKSMSGAIAILFFAWTINKIVGDMQTGKYLSSLVSGNIPMQFLPVILFVLGAAMAFSTGTSWGTFGIMLPIAAAMAANAAPELLLPCLSAVMAGAVCGDHCSPVSDTTILSSTGAKCNHIDHVTTQLPYAATVATATSIGYIVVGSL",
    "response#2": "MCVMMPVSSWGAYIITLIGGLLATYSITEYTPIGAFVAMSSMNFYAIFSIIMVFFVAYFSFDIASMVRHEKLALKNTEDQLEEETGTKGQVRNLILPILVLIIATVSMMIYTGAEALAADGKVFSVLGTFENTVVGTSLVVGGFCSIIISTLLIILDRQVSVPEYVRSWIVGIKSMSGAIAILFFAWTINKIVGDMQTGKYLSSLVSGNIPMQFLPVILFVLGAAMAFSTGTSWGTFGIMLPIAAAMAANAAPELLLPCLSAVMAGAVCGDHCSPVSDTTILSSTGAKCNHIDHVTTQLPYAATVATATSIGYIVVGLVLM",
    "response#3": "MCVMMPVSSWGAYIITLIGGLLATYSITEYTPIGAFVAMSSMNFYAIFSIIMVFFVAYFSFDIASMVRHEKLALKNTEDQLEEETGTKGQVRNLILPILVLIIATVSMMIYTGAEALAADGKVFSVLGTFENTVVGTSLVVGGFCSIIISTLLIILDRQVSVPEYVRSWIVGIKSMSGAIAILFFAWTINKIVGDMQTGKYLSSLVSGNIPMQFLPVILFVLGAAMAFSTGTSWGTFGIMLPIAAAMAANAAPELLLPCLSAVMAGAVCGDHCSPVSDTTILSSTGAKCNHIDHVTTQLPYAATVATATSIGYIVVGASLYSLLAAFSFGSATVFGKRILKSASFRTALYLRYLMTSCIMFVIVTFTSGFGDFLVATAGNWLIFVIIALTTGSGAILLYYFGLRYITAKVATMCELCFPISSVVFDYLINGNVLSPVQIASAILMIISIIKH"
  },
  {
    "reference": "MREILIFAILSFSVLSSKKILIYNEEVIVALSFVCFVIFSQKTFGETIKAIFDARSEALLSDLQQWMSYQEAMLSELKKQHELRSISLRSSTQMIGESCINDMVTRCAPKCKQTVKSVLCQQIEQKLKTLLAIQEHSRISLQEKIVTCFRETVCDEFRFSKLRKHQSKLVQQSMVLLKDGVPK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: This is one of the chains of the nonenzymatic component (CF(0) subunit) of the mitochondrial ATPase complex..2. The protein should have Helical domains to ensure proper insertion into the membrane.3. The protein should have a specific function in proton motive force-driven ATP synthesis and should be localized to mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "IGASIATLGLGGAAIGIALVFVALINGTSRNPSLRSTLFPQAILGFALSEACGLFCLMISFLL",
    "response#2": "IGASIATLGLGGAAIGIALVFVALINGTSRNPSLRSTLFPQAILGFALSEACGLFCLMISFLL",
    "response#3": "IGASIATLGLGGAAIGIALVFVALINGTSRNPSLRSTLFPQAILGFALSEACGLFCLMISFLL"
  },
  {
    "reference": "MNSQMSRLQKIRAEYRPHLPNVLHDLIKVYFEKGEPTTCVADQAEIEKHFPRTFGRPIVEGREGEPREGKKLKVGVVFSGGQASGGHNVIAGLFDALKSLCEDSELIGFMGGPSGIIDNKTLPITQELLAQYRNQGGFDLIGSGRTKIETQEQLNVSLKTVSERGLDGLVVIGGDDSNTNAAVLAEFFLEKGCKTSVVGVPKTIDGDLKNAYIATSFGFDTAAKTYAQMIGNIERDALSAKKYYHFIKLMGRSASHITLECALATHPNFTLIGEEVAAKNMTLSAVTKELTDVIEERSKGGKDYGVVLVPEGLIEFIPEMKLLIEELNALAAKESDLSPEKVGGMLGKEASHCFEALPEEIQKQLLLDRDPHGNVQVSRIETEKLLMSTVKKELANRSFKGKFSPLSHFFGYEGRAGFPSNFDTHYCYALGQTAALLVEGEWTGYMTCVKGFGRPVQDWSIAGLPITMLMNMEVRKGKEKPVIQKALVDLEGKPFAFFKENRSRWAVGDHYRYPGPIQFFGEGEMIDSIPLTHQFELL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a high specificity for substrate binding, with minimal off-target effects.2. The protein design should consider the endogenous proteins and enzymes present in the D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 3/4, Carbohydrate degradation pathway to avoid interference with native metabolic processes.3. The protein requires Mg(2+) to activate a critical catalytic site.4. The designed protein should have metal ion binding within cytoplasm to regulate fructose 6-phosphate metabolic process.5. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in beta-D-fructose 6-phosphate + diphosphate = beta-D-fructose   1,6-bisphosphate + H(+) + phosphate are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "IGVLTGGGDCPGLNAVIRAIVRKGVSVYGHEFSGFRDGWRGPVEGDARPLGIAQVRGILPRGGTILGSSRTNPYKVENGVDRIRENLEKLGVEALIAIGGEDTLGVAKKLTDEGIPVIGVPKTIDNDLGATDYTFGFDTAVTVAVEAIDRLHTTAESHHRALVVEVMGRHAGWIALHSGLAGGANVILVPEMPFDLDDVIKHVESRFATQYAPILVVAEGATPKEGAEVLKSGDLDAFGHVRLGGIGQWLAERIEERTGKESRAVVLGHVQRGGSPTPFDRVLATRFGLHAIDAVHEGAWGTMVALRGTDIVRVPLEAATGVLKTVPLERYAEAEVFFG",
    "response#2": "RVGVLTGGGDAPGLNAAIRGVARAAWREGYEVVGIRNGWQGLLRNDVFPLTPDAISGILPRGGTILGTSRTNPFKTSEGPRQVLDTVRTRGIDVVVAIGGDDTLGVAARLAKMGMRVVGIPKTMDNDIPGSDYTIGFDTAVTAVMEACDKLHPTAEAHQRVMVVEVMGRDAGWVAAVGGLAGGADVVLVPEVPFSVDEVCQRIKDRHERGKLFSVLVVAEGCKPKDLGTQVAQDAAVDAFGHVRLGGIGSVLAGEIEARTGYETRVTVLGHLQRGGSPTWFDRVLATRFGVAAVELIHEKRFGMMPALRGTEIVEVPLAEATATLKTVPAERYAEAEVFFG",
    "response#3": "IGVLTGGGDCPGLNAVIRAIVRKGVSVYGHEFSGFRDGWRGPVEGDARPLGIAQVRGILPRGGTILGSSRTNPYKVENGVDRIRENLEKLGVEALIAIGGEDTLGVAKKLTDEGIPVIGVPKTIDNDLGATDYTFGFDTAVTVAVEAIDRLHTTAESHHRALVVEVMGRHAGWIALHSGLAGGANVILVPEMPFDLDDVIKHVESRFATQYAPILVVAEGATPKEGAEVLKSGDLDAFGHVRLGGIGQWLAERIEERTGKESRAVVLGHVQRGGSPTPFDRVLATRFGLHAIDAVHSGAWGTMVALRGTDIVRVPLSEATAELKTVPAERYAEAEVFFG"
  },
  {
    "reference": "MISLGIEGTAEKTGVGIVDDEGNILGMAGKQLFPEKGGIHPRIAAEHHANWIPQLIPQAVEEAGISYDDIDLISFSQGPGLGPALRIVATSARTLALSLNKPIIGVNHCIGHVEIGKLDTGAVNPVSLYVSGGNSQVIAFESGRYRIFGETLDIAIGNCLDHFGRETGLGHPGGPVIEKLAKSGSYIDLPYVVKGMDFSFSGLLSAALREHEKGTAIEDICYSLQETAFAMLVEVTERALSHTQKDEVMLCGGVSANTRLREMLNTMAVEHGAKFYMPQMKLCGDNGVMIAWLGLLMHKQFGPMDIKDTGIIQRFRTDEVDIPWIKNEKSYLKLPDDLIAKGAESNIVKSSYLGRDAVLKNRVSKNYRIPEIDNKIRKARTKLEAKLLSDVKKAGVVTPILYDVNLHDKTILMEEIKGDLVKDIINEDLAYEIGENIAKFHNLNIIHGDITSSNMMVNDKNQLVFIDFGLGRYSDLFEDKAVDLLVLKKSLQSIDYNTAIKIFDKVLEGYADEYNDDSLNREQIIKKINEIESRGRYTH",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit ATP binding.2. The protein should be able to bind ATP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#2": "VRPKTLNQKRYVDAIDANTIVFGIGPAGTGKTYLAMAKAVNALQTKQVTRIILTRPAVEAGERLGFLPGTLSEKIDPYLRPLYDALYDMMDPELIPKLMSSGVIEVASLAYMRGRTLNDAFIVLDEAQNTTAEQMKMFLTRLGFGSKVVVTGDVTQIDLPGGARSGLRAAVDILEHIDDIYVAELASVDVVRHRLVSEIVDAD",
    "response#3": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR"
  },
  {
    "reference": "MEPVDPELEPWNHPGSQPKTACNNCYCKRCSYHCLACFHKKGLGISYGRKKRKQRRRAPQSSEDHQKPISEQPLSRTQGDPTGSEESKKKVESKTETDPFD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that contributes to suppression by virus of host translation initiation, modulation by virus of host chromatin organization by functioning in extracellular region, host cell nucleolus with cyclin binding, RNA-binding transcription regulator activity, metal ion binding, trans-activation response element binding.2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.3. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.4. The designed protein should contain at least one Nuclear localization signal, RNA-binding (TAR), and protein transduction motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "LVSEGL",
    "response#2": "LQCFLCNAP",
    "response#3": "MASIRL"
  },
  {
    "reference": "MENPLCVSIFLFYCILIQSSAHGQSLGPESFGRRSRAAETNKTPQETRTKFLLFRGETDKGCQILLNHSDTLQQCGFNSSLPLVMIVHGWLVGSADTPFFSLLLLFFFSPSLVCSILITITRTVKCAHERSVHLFIDSLLHADMQSMAYLCRDMDRFSQGLCLSCKKGRCNTLGYHTRQERQSKKSKSLFLVTRAQSPFKVYHYQFKIXFINQTENPVEPTFTVSFLGTRGERQKIPITLSKGITSNETYSFLITLDVDIGELIMIKFKWENRMVWSNVWNTVQTIIPWGRGSLHSGLALKSIXVKVGETQQRMTFCSENMNDLLLHPAREKTFVRCEANSETLKRKIR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. We are looking for a protein with broad substrate specificity for a triacylglycerol + H2O = a diacylglycerol + a fatty acid +   H(+).2. The protein must be able to triglyceride lipase activity, lysophospholipase activity, phosphatidyl phospholipase B activity.3. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "SVHNLRPADIQVIAALGDSLTAANGAASKPYDVLDVLTQYRGLSWSVGGNENISTVTTLANILREFNPSLIGYSIGTGRETTDNAALNQAVAGDCAEDVPAQVRRLVDLMKNDTRVNFQTDWKLITLFIGGNDLCRFCRNPVRYSPENYTYNLQVSLDMLHREVPRAFVNLVTVLYIVSLRELHTSENNTCPKLLMRLLCPCVLSPEDNSDEFKKLVYFNRRYQERTRQLVESGRYDTTDDFTVVMQPFSVNINMPKTQEGLPDSSYFAPDCFHFSQKTHSQAARALWNNMVEPLGKC",
    "response#2": "SVHSLKPADVKIIAALGDSLTAGTGIASDSLLDLGTEYRGLSWSIGGDASLENMTTLPNIFREFNVTIVGYSTGTGSANESNAFLNQAVPGAETEHLPAQARNLLRLMQTDPRIDFSADWKLITVHIGGNDLCNYCKDPDHYSAVNFTRRIQETLDILHEQASAVPKALVSLVEVMDLHPLRQLFVDNQAQCPTYMADYLCSCVLTGEENSQNLAMVKEATRDYQLGIWRLLESGRYDTHEDFTVVIQPFLQNPKIPLGQDGHPDISYFAPDCFHPSQKGHSQLAKALWNALLQPIGA",
    "response#3": "SVHRLRPSDIKVIAALGDSLTAGRGAQGGLPGLLVDYRGLSWSIGGDGDYSHIATLPNILRQYNNKLYGYSEGVASFKSRLNMAKTGAMSDEILEQAQNLVQRLKDDKHVDIANDWKLITIFIGANDLCDWCIDEDKFSAVNYRRNLEETLDYLFANVPRAFVNLVEILDLEFINILDTTPICKFVHKLVCDCASNPKTPEDRDKLIKLREGYQAVTRQLVSSGRYDSSDNFTVVLQPFYENTTIPLKKTGKPDFSYFGSDCFHFSKKSHVASAEALWNNMIEPVGRC"
  },
  {
    "reference": "MSRSIKKGPFADASLLKKIEAQEGSEKKQVIKTWSRRSTIFPSFVGFTIAVYDGRKHVPVYITEDMVGHKLGEFVPTRTFRGHKVADKATTTVGK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cytoplasm that is critical for translation. The designed protein sequence is ",
    "response#1": "LKTEFASLRTGRASASMLEPVMVDAYGSMTPINQVGTVNVPEPRMVTINVWDKGLVGKVEKAIRESGLGINPQLNGTIIMLPIPELNEERRRELTKVAGQYAEHARVSIRNVRRDGMDQIKKAKADGMSEDDQKFWETEVQELTDKMIKAVDAALETKQAEIQ",
    "response#2": "LKTEFASLRTGRASASMLEPVMVDAYGSMTPINQVGTVNVPEPRMVTINVWDKGLVGKVEKAIRESGLGINPQLNGTIIMLPIPELNEERRRELTKVAGQYAEHARVSIRNVRRDGMDQIKKAKADGMSEDDQKFWETEVQELTDKMIKAVDAALETKQAEIL",
    "response#3": "LKTEFASLRTGRASASMLEPVMVDAYGSMTPINQVGTVNVPEPRMVTINVWDKGLVGKVEKAIRESGLGINPQLNGTIIMLPIPELNEERRRELTKVAGQYAEHARVSIRNVRRDGMDQIKKAKADGMSEDDQKFWETEVQELTDKMIKAVDAALETKQAEIM"
  },
  {
    "reference": "MNSKNTIVYVKVKGKRPDGFVDPPVFEWNIQKEKKLWSLLSQLNDPDKEIDWALLAEGLDAPIYFLKRRCYELFTKHFELLRQQIDRKNKNALTDRSETPERDSTEEITKEVNFLNQLPVRTPGTIQEEFNEDNGPKDKNKTPDTTKKAIEQLKSSRILNFKGNKLDYNNREVGLTRYDSTYEKEGVHTEETESELSSSLGVSKSTLEEALMDKLQI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be optimized for stability and expression in phagophore assembly site where it will participate in piecemeal microautophagy of the nucleus, protein transport, late nucleophagy, protein localization to phagophore assembly site, autophagy of mitochondrion.2. The protein must exhibit the following characteristics: Also plays a role in mitophagy and regulation of filamentous growth .; Plays a role in autophagy; Functions at the preautophagosomal structure (PAS) in order to form normal autophagosomes under starvation conditions.3. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "VSHFFPKALHIVLNSRIPSLQSRGRTRERLSGLNVRKSDKWFNLVMGDRPAALEKLHSWHRNILDSMIIDIILVHPISNDNLDDDDDHSDSVVRSAETVIERWVVQYENPLIMSPQSSDSATRYQKVYKKSIILLRSLYAQTRLLPAYRVSRQLSSSLASSGYDLIYKVSSFSDIFSGPVTETMKEFRFAPVEVPPGRLCASVTYRSDQ",
    "response#2": "GGFFLKATLLVCQPRTGAIEGDGEEDRTFNISSAADAGVAEAVRAWTAYDGAQELPPLVIEMFLDLRRLGPQHTVVLKDQDGNPWNVCKGGKKSEIMLERWLVELDVGNEEARPEEPPNAAEIYKQLVLLFRYLYTLTQLLPANELYARLHGPSNQGEAAMPLINIGTRILDGSKPILSKGRVGLSRSIIATYTNVINETNVASHLEQKKITPISTGYGSLRITVSYRRDCNG",
    "response#3": "SLSSINHVWRANEIVTAARAALEECVVVSARTAFLRRGLNNQLRLLYSVRTEVEEISLRGRSEFAAVLKDLDDADTRLRRTLELLRETIVHPAFRPEGEDPKSLHDFVDERGVEELHAALKSSIDRTTAAQAQLDSSNHAFDDELLSIKEALGTYRTAAKLASSRSSSSASSSSTSNSSLPSISTMPSMLHSLEMHAQEMANLLESLVRHFDLCVTAVKHTEGGGAAAKSITGDMPVGVPVSGRMGSNIEEEINNNLNAPLDPLSNSEYQEMVNVLFKDAAEAEDVVMEIQDRIGEMESVLENVLAQRDALRSIYNATTDIYQHLSSLGSTRLPGYIAQAHNFTQVWHEENDRISGGLADLSDLNSLYNGFLDAYDGLIVEVARRKHVRQRVEKVLRDTRHKLDQLYEEDVTARETFRVEKGDYF"
  },
  {
    "reference": "MARPRPGSDSPLSRLVRGVLKSMSTPIPLNDLLRRAGLNPVEGGEECLITSVTQDSRRVRPGSLFLALPGNSRDGLEFVEDALARGAVAVAAPLPLGLGLPTVVLPEGRKSAALLAAACEGNPASSLDVYAVTGTNGKTSTCYFFEAILKEAGRRPGLVSTVANRTGGKERASHMTTPPPEDLHAYFAQMKRSGLDSAVVEASSHALDQDRLAGIEFAHAAITNITREHLDYHRDLFSYREAKARLPGLIRPGGALVMNLDDPHVAYFADRCPEGVRFTGFTSRDRDDALLAARLDGMTLDGMTMALRTADGKSARLTTSLTGLHNKDNLLTAASLGLLAGIDLATIVRALGRVEAPPGRLEEIAVHAGFRVFVDYAHTPDALEKVLGALRSVAPRRLLLVFGCGGEKDRGKRPAMGRIAECLADRALITDDNPRGERPEAITDEIRAGVSNPGAMPYIRDRKEAIEAMLSEARPGDVVLIAGKGHEGEQIIQGRRIPHDDRAVVRAWRDERERSIERAT",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein with UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, magnesium ion binding is needed to enhance cell wall organization, cell cycle in a specific context.2. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.3. The designed protein should contain at least one Meso-diaminopimelate recognition motif motif to achieve its intended function.4. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.5. The UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP, meso-2,6-diaminoheptanedioate binding site should be located in a region of the protein that is accessible to the ligand.6. Ideal protein should have a binding affinity for key enzymes in the Cell wall biogenesis, peptidoglycan biosynthesis pathway to enhance the overall efficiency of the pathway.7. The protein contains novel Mur ligase N-terminal catalytic, Mur ligase C-terminal, Mur ligase central domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MNKITGIKINSQEVCPNDIFVAIRGQTNDGHDYILECINKGVKTIIYEDHYYDLIPLENVNMIRTNCTKTTLAVLASYFYDHPSKELKLIGVTGTNGKTTTTYLIDAIYTHAGIRTGTLGTTGLKVGDSLQALPNTTPESLTLQQAFRDMVDQQVTAGIMEVSSHALVTGRTRGCQFDVAIFTNLTQDHLDYHQTLTAYQDAKRLLFASLPAKTKTGKPTFAILNVDDKASAGFRDVTQGLVVTYGLENEADFSASQLVLKSKGTTFNLKTPDGFFAMTLPLAGKFNVYNALAAAAAASLELGIPLSLIANSLANFKAVEGRMELVGKTKTGASVIVDYAHKPLALETVLKSLRAHVQGQIIVIFGCGGNRDQAKRKMMGEIAKDYADSIIVTDDNPRFEDPQAIRKQILEGVPEAYEIASREEAIAFALHKAEAGDICLIAGKGHEDYQILENETIHFDDREEAKKALKERFG",
    "response#2": "MTITGLAVDSRDVKPGFLFAALPGARVHGGEFIQYALRMKAGAILTDAKGAEIAADELAASDAAVIVAQDPRQALAQTAALWFGAQPEQMVAIGVTGTNGKTTTTYLIERILSDAGQRAGLVGTIQYMVDGKQMESANTTPEALDLQRLFAEMRDAGCHVRGITHDSRKVGPGDLYAALPGTRAHGADFAAHAAGAGASAILTDPAGAERAAATGLPVIVVPDARAALGRAGIVTGTNGKTTVSCLVRELMEYKGRRTGLIGTIEHILGENRIIDSFTTPDAVLLQKYFAEMVKQNLSTAVTEVSSIGLSLGRVRETKFLAGVLTNISLDHLDFHGSFEEYVAAKQQLFAALPEQGIAVVNLDCQYAQSFLASSPARGVSYAVHQEADYRAINLRFSSSGSSCDIWYQGEVFPCETSLVGEHNVYNILAALAVAIACGAEPERALAALPGLKGVPGRFQYTVSTEGVTAIVDYAHTPDALKNVLQTINDIRTGNETVITVIGCGGDRDVAKRPKMAMIASQLSDQVIFTSDNPRTEDPQSILDQMEVGVAAENYKKTLSILDRRQAIKTACKLAKVGDIILVAGKGHEDYQQIGREKRPFSDQAEAIAALAARGVI",
    "response#3": "MKLKDILYKVSLLEIIGNTNVITGLNNDSRSVQPGTLFFCLPGYKVDGHDFAAAAASNGAAALVVERKLDVPDNACLIADVNPRRAFAELSANFYNYPAKKLKLIGIGVTGTNGKTTTTYLIERILSDAGKKTGLIGTIENIIGNKTIPANNTTPESYIIQEYFAKMVDAGCDSVVMEVSSQGLMLHRVDDCDFDIGVFLNIGEDHIGPNEHKDFDDYLACKGLLFKQCKIGIINRDDQYTDLVIKNATCKIETFGFSKNADYKAENLELVEGHPGITFKVTTPHGLLEADIAMAGKFNVYNALAAISTCISEGIDFETIKQGLETAKVKGRMELVGKTKTGASVIVDYAHKPLALETVLKSLRAHVQGQIIVIFGCGGNRDQAKRKMMGEIAKDYADSIIVTDDNPRFEDPQAIRKQILEGVPEAYEIASREEAIAFALHKAEAGDICLIAGKGHEDYQEIKGVKHHFDDREVVREIFREITGLNNDSRSVQPGTLFFCLPGYKVDGHDFAAAAASNGAAALVVERKLDVPGRLDPVLSGPCPVYIDYAHTPDALDNVCKILSQLLPKDGRLIIVFGCGGDRDRSKRPLMAKVSERYGFSVVTSDNPRTEDPERIIADICSGFSTDCYVVEGDRKLAIIKAMSMASDKDIVLVAGKGHENYQEIKGVKHHFDDREVVREIFRE"
  },
  {
    "reference": "MSYSSQLDALGIEKYLEKYGEREMLKLLTCGSVDDGKSTLIGRLLYDSEQVHDDVLAATTKASSKFGTTGEEIDLALLVDGLQAEREQGITIDVAYRYFATDRRKFVIADCPGHVQYTRNMATGASACDLAIILIDARHGVLEQTHRHSFICSLLGIQHFVIAVNKMDLVDYSQERFDEIREAYSEFAARLQVRDIHFIPMSALRGENVVHRGESMPWFQGSPLLDHLETVHVASDRNLVDLRFPVQNVLRPNLDFRGYAGTIASGVVRVGDAVTVLPSGKQSTVKSIETFDGQLQEAFAPMACVLTLDDEIDISRGDMIATPNNAPTLANDIEAMVVWFDEKPMTPGQNLLIKQTTAQTSATIADLRYRINVNDLHRESAPELKMNEVGRVHVECARPLAADAYTKNRATGAFILIDRMSNATVGAGMIVERRIAKDALARRSESADAGSNVRLQDKRKVNAEQRRQRLGQEPFVLWITGLPRAGKSSLAYELERRLFEQQRFVQVLDGEILRLGLNRDLGFSGADRWENQRRAAELARLNTSMGVSTIVALVSPLAFERQRAREIVGDRFVEVFCNAPLEVCEARDEHGLYERARAGEIANVTGIDAPYEAPDAADVVLDTEHDSLDDNVQKVLDHLAAHGLLDQPKPLDD",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must be optimized for GTP binding, GTPase activity.2. For general function, the protein need meet that With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway3. The GTP binding site should be located in a region of the protein that is accessible to the ligand.4. The presence of Tr-type G domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "KTMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGGEIDFALLVDGLAAEREQGITIDVAYRFFATEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSYLVNLLGIRHVVLAVNKMDLVDYNEDIFHQIVKDYGAFARSIGITDFTAIPISGYRGDNITAASENMTWYNGTALLPFLESVDVDVQDLLRFITCGSVDDGKSTLIGRLLWESQQVFDDQLSTLQADSRRHGTQGNDIDFALLVDGLAAEREQGITIDVAYRFFSTPRRKFIVADTPGHEQYTCNMITGASTADVAVLLIDARQGVMTQTRRHAYLAALVGIKHIILAINKMDLVAYGHQLFADTLAAFNTAVEPLGFDSITAIPLSALKGDNINSRSAHTPWYQGPTLMACLETIDPRPLRLPVQWINRPNQNFRGFAGTIAAGQVQAGDAVVALPSGRRSTVTQVLGPDGAVAQAGTGQAITLTLADEIDISRGDVIAHAEVADQFEAHLVWMSEEQMLPGRTYLLKLGTSTAVAQVGQPKYAVNVNTLERTAARTLGLNEIGVVALHLDRPIPFDPYAGNRDTGGFVLIDRFTQDTVAAGEVADQFEAALVWMDEVEMVPGRAYMLKVGAQTVSATVHQPKYEINVNTQEHLAAKTLDLNAIGIANITTDREIPFAPYAENRDLGGFILIDKLTNRTVAAGMITHGLRRAQNVHWQAVDVDKAARAAQKGQSPCIVLAVNKLDEVADQFAAHVLWMDDAALLPGRPYWLKIGTRTVSATVSEIKHRVDINNQEHLAAKRLELNEVGYCNLSLEEPVVFAPYARNRTLGGFILIDRQSNRTVAA",
    "response#2": "KELLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSARVGTQGKAIDFALLVDGLAAEREQGITIDVAYRFFATEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLVQTRRHSYLCHLIGIRHLVLAVNKMDLVDYDPAVFDRIVAEYRAFAREIGIADVTAIPLSGFKGDNITSAPSANTPWYSGPSLIEHLETVEIEARFITCGSVDDGKSTLIGRLLWESQQVFDDQLSTLQADSRRHGTQGNDIDFALLVDGLAAEREQGITIDVAYRFFSTPRRKFIVADTPGHEQYTCNMITGASTADVAVLLIDARQGVMTQTRRHAYLAALVGIKHIILAINKMDLVAYGHQLFADTLAAFNTAVEPLGFDSITAIPLSALKGDNINSRSAHTPWYQGPTLMACLETIDPRQDTLRFITCGSVDDGKSTLIGRLLWESHHIFDDQLAAVTAASKRHGTQGDNIDLALLLDGLSAEREQGITIDVAYRFFGTARRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGLLMQTRRHARLVSLMGIRHITLAINKMDLVGFDAKVFERIAHDFGTLAEGLQFGTVTAIPISALQGDNITTRSARTPWYSGPTLLGYLETAQIHPAQFTATVIWMHEDKGMVGRNYELKLAGQWSGASLTSIKHRIDVNTGAHESCRQLGLNDIAACNLALSRPVVFDAYITSHTLGAFILVDRFTQATVAAGLIA",
    "response#3": "DLLRFLTCGSVDDGKSTLIGRLLHDSKMIYEDQLAAVEADSATVGSVGGDLDLALLMDGLKAEREQGITIDVAYRYFSTARRKFIIADTPGHEQYTRNMVTGASNCQLAIILIDARNGVRPQTKRHSYIASLLGIKHIVVAINKMDLVDYSKDRYEEIRDHYMECGERLGITDPFFIPMSALRGDWLAALL"
  },
  {
    "reference": "MRYLIAILSLLSLANAQFGGFFDQMFGQQGGHEQHHGQPQNNPSDASHYRHQYDNSVCDKYLCPDTLACVHFPHHCPCAWDAQQEKFELAEGQRMCVSRGGFREGETARKLELARKGLL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Probable component of the endoplasmic reticulum-associated degradation (ERAD) pathway.2. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "VNMVQLPGGRFQMGTDSPDGRDGEGPVREVTVKPFAIDIFPVTNKDFREFVREKKYRTEAEVFGWSFVFEDLVSDELRNKATQRMQSLLWWLPVERAFWRQPAGPGSGIREKLEFPVVHVSWNDARAYCAWRGKRLPTEEEWEFAARGGLKGQVYPWGNKFQPNRTNLWQGKFPKGDKAEDGFHGVSPVNAFPPQNDYGLYDLVGNVWEWTASQYQAADQDMRVLRGASWIDTADGSANHRARVTTRMGNTPDSASDNLGFRCG",
    "response#2": "VNMVQLPGGRFQMGTDSPDGRDGEGPVREVTVKPFAIDIFPVTNKDFREFVREKKYRTEAEVFGWSFVFEDLVSDELRNKATQRMQSLLWWLPVERAFWRQPAGPGSGIREKLEFPVVHVSWNDARAYCAWRGKRLPTEEEWEFAARGGLKGQVYPWGNKFQPNRTNLWQGKFPKGDKAEDGFHGVSPVNAFPPQNDYGLYDLVGNVWEWTASQYQAADQDMRVLRGASWIDTADGSANHRARVTTRMGNTPDSASDNLGFRCG",
    "response#3": "GTVIGIDLGTTYSCVGVMQKGKVEILVNDQGNRITPSYVAFTDEERLVGDAAKNQAAANPHRTIFDIKRLIGRKFSDKDVQNDIKHFPYKVVSKDDKPVVKVEVKGEEKTFTPEEISAMILGKMKETAEGYLGKKVTHAVVTVPAYFNDNQRQATKDAGMIAGLNVLRIVNEPTAAAIAYGLDKTGEERQIIVYDLGGGTFDVSLLSIEQGVFEVLSTAGDTHLGGEDFDQRIINHFAKLFNKKHGVDVTKDAKAMGKLKREAEKAKRTLSSQMSTRIEIEAFYDGKDFSETLTRAKFEELNNDLFKKTLKPVEQVLKDAKVSKSEIDDIVLVGGSTRIPKVQALIEEFFNGKKASKGINPDEAVAFGAAVQAGVFDKN"
  },
  {
    "reference": "MEFSSPLQRVSIMAASLTGLLLLQAASWASGAHPCVPKSFGYSSVVCVCNATYCDSLDPLTFPALGTFSRYESTRSGRRMELSIGTIQANHTGTGLLLTLKPEEKFQKVKGFGGAMTDAAALNILALSPSAQSLLLKSYFSTEGDGGFPCTFSPFPDIWFLSLWKIRLPSLQLAHRPVSLFASPWTSPTWLKTNGAVNGKGHSRVTRDIYHQTWASTLFLDAYAEHRLKFWAVTVENEPSAGLISGYPFQCLGFTPEHQRDFIARDLGPTLANSTHRDVQLLMLDDQRLLLPHWAQVVLADPEAAKGLCGLQVLGAEREARLLGSRSAVQPQHYHEPPLPCGCPIIVDIAKDVFYKQPMFYHLGHFSKFIPEGSQRVGLVASKKNDLETVALMHPDGSAVVVVLNRSSKDVPLTIKDPAMGFLETISPGYSIHTYLWRRQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein have Glycosyl hydrolase family 30 beta sandwich domains with specific sequence motifs that are crucial for its activity.2. The designed protein should have glucosylceramidase activity within lysosomal membrane to regulate cholesterol metabolic process. The designed protein sequence is ",
    "response#1": "VKGFGGAITDAAALNILALSPPAQNLLLKSYFSEEDEEEGDKMTVCDFSIRTYTYLHNSSLPEEDTKLKIPLIHRALLKISGAGNGKVSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTVENEPSAGLLSGYPFRCLDFIAHDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVIRPRPPR",
    "response#2": "VKGFGGAITDAAALNILALSPPAQNLLLKSYFSEEDEEEGDKMTVCDFSIRTYTYLHNSSLPEEDTKLKIPLIHRALLKISGAGNGKVSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTVENEPSAGLLSGYPFRCLDFIAHDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVIRPRPPV",
    "response#3": "VKGFGGAITDAAALNILALSPPAQNLLLKSYFSEEDEEEGDKMTVCDFSIRTYTYLHNSSLPEEDTKLKIPLIHRALLKISGAGNGKVSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTVENEPSAGLLSGYPFRCLDFIAHDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVIRPRPPG"
  },
  {
    "reference": "MLNGKRILLIVTGGIAAYKTPELVRQLIKAGATVRCVLTRGGAQFVTPLALASVSGDQVYSDLFSLTDEHEMGHINLSREADVVLVAPATANTLAKMAHGLADNLSTTVLLATDKPVLAVPAMNVRMWEHAATKSNIAMLRARGVTMIGPDEGEMACGEWGEGRMAEPEDIVLALSSFFGSTAPLGGYSAIVTSGPTHEPIDPVRYIANRSSGKQGHAIAQALKELGARVTLISGPVDLPDPQGVKVIHVQSARDMATAVEGALPADIAVCAAAVADWRVDNQAPQKLKKGIDGMPSLSMVENPDILAALSRPGERRPQLVVGFAAETENVLEHARAKLAKKGCDWICANDVSPSTGTFGGDQNTVQLVTRTSVEDWPSQSKFHVAKQLATRIAAHLGAQKFAHKPGEAS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be optimized for FMN binding activity in order to improve coenzyme A biosynthetic process. The designed protein sequence is ",
    "response#1": "QDQRVVVTGGGSVEAIDSVRCISNISSGLQASSLALALWLKGAQVVFISSAFALLPEGITCISVKSAQDYLKALKSEQNFYNPPFLFMLAAVSDYKSAHVYTGKLKKQDLGSTWNLECVQNPDILKSLEGFYKIGFKAEESIESLASAQKLLEPVDLGGKGCKVVCLNSTQALGATNNQIVRL",
    "response#2": "QDQRVVVTGGGSVEAIDSVRCISNISSGLQASSLALALWLKGAQVVFISSAFALLPEGITCISVKSAQDYLKALKSEQNFYNPPFLFMLAAVSDYKSAHVYTGKLKKQDLGSTWNLECVQNPDILKSLEGFYKIGFKAEESIESLASAQKLLEPVDLGGKGCKVVCLNSTQALGATNNQIVGF",
    "response#3": "QDQRVVVTGGGSVEAIDSVRCISNISSGLQASSLALALWLKGAQVVFISSAFALLPEGITCISVKSAQDYLKALKSEQNFYNPPFLFMLAAVSDYKSAHVYTGKLKKQDLGSTWNLECVQNPDILKSLEGFYKIGFKAEESIESLASAQKLLEPVDLGGKGCKVVCLNSTQALGATNNQIVLA"
  },
  {
    "reference": "MSGQDPVKESGQREPIAVVGSGFRFPGSSNNPSKLWDLLVKPRDLLTKIPENRFNSDAFYHPKPFHHGTSDVRESYFLEEDHRQFDAAFFNIKPVEVHAIDPQQRILMEAVYESLEAAGLSMESLAGSRTGVYVGLMCADYVDLLNNDVNSLPTYTPTGTARSIMSNRISYFFDWHGPSMTIDTACSSSLVAVHQAVQLLRSGDSDVAVAAGANLMLGPLPYIAESKLQMLSSNSRSRMWDIDASGYARGEGVAAVVLKRLSSAIADGDQIECIIRESGINQDGRTKGITMPSSVAQADLISRTYAKAGLNPRDPTERCQYFEAHGTGTAAGDPKEAEAISKAFFHPGEDISGKTDPLYVGSIKTVIGHTEGTAGLAGLLKASLAVQHGIVPPNLLFNQLSPAVEPFYTNLEVLTSPRPWPKLAEGTPRRASINSFGFGGTNAHCIIENYIPSPAHTDRAITTRQFTPFNFSAASEKSLRGILTDYSNYLRLNPEVSLQDLSYTLYARRSEHAVRVHISAGSTTDLYTRIDDLLQVPSSGGNAQSIGTRSKILSRPVRALGVFTGQGAQWPSMGRELVLNSPYAKEVVQKLDLVLQSLPEPERPDWSLMYELTCDASQSRLNTAVIAQPLCTVVQIILFDLLSSAGVKLQAVVGHSSGEIAAAYAAGYLTREDALKIAYYRGYFTNLTPSDRPGAMMAIGTSAEDAEELCSLPMFQGRLAVAAVNSSSSVTISGDRDAIEQAKEVLEDEKKFARLLKVDKAYHSSHMV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: XenF catalyzes sigmatropic rearrangement to form an A-ring, which leads to an unusual intermediate with a hexane ring, which is required for the formation of the tricarbocyclic product; The alpha/beta hydrolase xenA then accelerates intramolecular nucleophilic attack to give a pyrrolidone derivative; Hybrid PKS-NRPS synthetase; part of the gene cluster that mediates the biosynthesis of xenoacremones such as xenoacremone A, a compound that shows inhibitory activity toward the PI3K/AKT signaling pathway and which has the ability to induce apoptosis of A549 lung cancer cells.2. The designed protein must be optimized for 3-oxoacyl-[acyl-carrier-protein] synthase activity.3. The designed protein must have at least one Malonyl-CoA:ACP transacylase (MAT), Ketoreductase (KR), Carrier domain.4. The designed protein's functionality will benefit from Basic and acidic residues, Polar residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "IAIIGSSCRFAGGASSPSKLWDLLLKPKDLSKPPPESRFNLEGFYHPNAEQHGNTNIHGSYFLEEDPRCFDTVFFNISPKEAEAIDPQQRILLEVVYEAMEAAGLTLQGLQGSDTSVYAGLMIRDYMDVQVRDPDFFSQYMVTGTSSALNANRISYFFDWHGPSLTVDTACSSSLVAVHQAVQGLRAGESRVACVTGSNLLLGPELFISASNMHMLSSRSKMWDVSAEGYARGDGFASFMLKTLSNAIADGDHIEAIIRETGVNSDGRTKGITLPSPQAQADLIRDTYRRAGLDLSNPNDRCQYFEAHGTGTQAGDPREASAIHDAFFGAEGDDNKTGPKLVVGSIKTIVGHTEGTAGMAGMLKALLAIRHRVIPPNLHFNNLNPSVAPFYDRLTVPTESIPWPVVAPGTPLRASVNSFGFGGTNAHAIVESYEAPALCAPPPQFLEELAKEVPQGAVEFRKRLASLEDNTDNGPVVLNFTDGTRAEVDAVIGCDGIKSVVRKQMFGTNHPASNAQYTHKVAYRGLVPMNRAVEVLGPWKAGNFHHHVGPGAHLTHYPVANNTVLNVVAFLSDPNPWPDNQRMEMEGSREDVLTGLKGWHPTVLNLVNLLPEKLSKWALFDLCEFPAPSYSAGRVCIAGDAAHASSPHHGASACLGVEDCLCLNVLLAQVRG",
    "response#2": "NDDIAIIGMGFRFPGGGNNPDQFWNQLSNKMDGISKISQEKWSRSFYEQKYINNEYGGVLKDEEWKNFDPLFFGISPKEAPTIDPQQRLLMTTLWEAFEDANIKPSTLRGSDTAVFIGMMNLDYQRCQFRDISYINPYTVTGSAGSFVSNRLSFSFDLRGPSMTLDTACSSSLNAVYLGCQAIATGDSKMAIVGGVNGIFDPSISMTFSGLNMLGHKGQCRSFDAGADGYIRSEGGGVCILKKYSDAIKDGDRIYCVIKGGSSNVDGYNAKTNITQPSMKAQGENIEIALKKSGVNPSDIYYIEAHGTGTPVGDPIEIEAISRIFKDNHTPDAPLYIGSVKSNIGHLESAAGIASLIKVALSLKNRSLVPNIHFEKPNPLIKFEDWNIRVVTDEIQFPTNKLINMGINCFGLSGSNCHMILSSNPKPKLPKLDQFLEELAKEVPQGAVEFRKRLASLEDNTDNGPVVLNFTDGTRAEVDAVIGCDGIKSVVRKQMFGTNHPASNAQYTHKVAYRGLVPMNRAVEVLGPWKAGNFHHHVGPGAHLTHYPVANNTVLNVVAFLSDPNPWPDNQRMEMEGSREDVLTGLKGWHPTVLNLVNLLPEKLSKWALFDLCEFPAPSYSAGRVCIAGDAAHASSPHHGASACLGVEDCLCLNVLLAQVRQDDGPCPLFVHVFSGQGPQHIFMGRQLAASYPAFLDAVRENDRVLVERYGQESMLERTGLFVPGVECKLAANGQWPVQEVVLSLVFVQIALVDLIKSLGIQYDFVMGHSIGEIAMGYASGHYDREMAVGIAVARAAAMTKAEGNGAMVALGVGVQKAKVMIRKVFSKYKVTSGLWIAGINSPQAVTVAGTHELIDAMVELAADPDAKVFAAKLRVGCAFHTPLMEPQEELFKSRMAD",
    "response#3": "IAIIGSSCRFAGGASSPSKLWDLLLKPKDLSKPPPESRFNLEGFYHPNAEQHGNTNIHGSYFLEEDPRCFDTVFFNISPKEAEAIDPQQRILLEVVYEAMEAAGLTLQGLQGSDTSVYAGLMIRDYMDVQVRDPDFFSQYMVTGTSSALNANRISYFFDWHGPSLTVDTACSSSLVAVHQAVQGLRAGESRVACVTGSNLLLGPELFISASNMHMLSSRSKMWDVSAEGYARGDGFASFMLKTLSNAIADGDHIEAIIRETGVNSDGRTKGITLPSPQAQADLIRDTYRRAGLDLSNPNDRCQYFEAHGTGTQAGDPREASAIHDAFFGAEGDDNKTGPKLVVGSIKTIVGHTEGTAGMAGMLKALLAIRHRVIPPNLHFNNLNPSVAPFYDRLTVPTESIPWPVVAPGTPLRASVNSFGFGGTNAHAIVESYEQFLEELAKEVPQGAVEFRKRLASLEDNTDNGPVVLNFTDGTRAEVDAVIGCDGIKSVVRKQMFGTNHPASNAQYTHKVAYRGLVPMNRAVEVLGPWKAGNFHHHVGPGAHLTHYPVANNTVLNVVAFLSDPNPWPDNQRMEMEGSREDVLTGLKGWHPTVLNLVNLLPEKLSKWALFDLCEFPAPSYSAGRVCIAGDAAHASSPHHGASACLGVEDCLCLNVLLAQVRG"
  },
  {
    "reference": "MIQTQNYGTGRRKSSSARVFLRSGNGEIVVNKRSLNEYFGRETSCMIVRQPLELVDMVDKFNIYITVKGGGISGQAGAIRQGITRALIKYNQTLRFELRKAGFVTRDSRQVERKKVGFRKARKRPQFSKR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be optimized for stability and expression in ribosome, ribonucleoprotein complex where it will participate in translation. The designed protein sequence is ",
    "response#1": "MTPPTSALVLSELKLEKGSGTPNTNFIGSLTIEQAIKVAQMKRDAMLSYTLKNAVKEVAGTCVSLGVMS",
    "response#2": "MSFLGL",
    "response#3": "ETPPTSALVLSELKLEKGSGTPNTNFIGSLTIEQAIKVAQMKRDAMLSYTLKNAVKEVAGTCVSLGVML"
  },
  {
    "reference": "MSLGNARPLVSVYTEKNEPAKDKNICLPAVFKAPIRPDVVNEVHQLLRRNNRQAYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTFRRWHRKVNVNQRRYALVSAIAASGVPALVQSKGHVIDGVSEFPLVVSDEVQKVQKTKQAVIFLRRLKIWADIQKVYKSQRFRAGRGTMRDRRRIARRGPLVVYDKDEGLRKAFRNIPGIETINVDKLNLLKLAPGGHVGRFVIWTESAFARLNDLFGTWKKPSTLKKGYNLPQPKMANTDLSRLLKSEEIRKVLRDPRKRVFRSVRRLNPLTNVRQLIKLNPYAEVLKRRAALAAEKRTVAKVLAKAKKQNVELAKSHFANVATKAAANRAKLLAARKKKVAAKKPAAKK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have structural constituent of ribosome, RNA binding and be active in cytosolic large ribosomal subunit, cytosolic ribosome. The designed protein sequence is ",
    "response#1": "GIPN",
    "response#2": "ECVVA",
    "response#3": "MSII"
  },
  {
    "reference": "MNHYKDFISNLIENLGSENVKTNELMKNHTSFKVGGPVDILVTPESYEQVQYVIKHSRGNNIPYFIMGNGSNLLVRDGGIRGLVIKFCKLNRIKIEDDKIIAQSGVLLSKVSNMAAKNNLEGLEFASGIPGSIGGALTMNAGAYNGEISQVIDSALVLDKSGEILNLSKEELELGYRTSSILKNGYVVLEAILKLSLGDSKNIYDRIKELTEKRKTKQPLEYPSAGSTFKRPQGYFAAKLIEESGLKGINVGDAEVSQKHSGFIINKGNASAKDILNVINIVQDTVKSKFDVELHTEVLIIGEDKLN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The Proton donor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.2. The designed protein must be optimized for FAD binding. The designed protein sequence is ",
    "response#1": "GEVYLVGAGPGDPDLLTFRALRLMQRADVVLYDRLVAPAIVDLVRRDAERIFVGKEKSNHVVPQPEINELLLRLAQQGKRVLRLKGGDPFIFGRGGEEIEDLVRHNIRFQVIPGITAAAGCASYAGIPLTHRDHAQSCLFVTGHLKDGSVDLNWNALCQPRQTVVVYMGLTGLEVICREMIRHGRERSTPAALIQQGTTLNQQVITATLESLPDAVAAAQVEAPTLLIVGSVVTLY",
    "response#2": "GEVYLVGAGPGDPDLLTFRALRLMQRADVVLYDRLVAPAIVDLVRRDAERIFVGKEKSNHVVPQPEINELLLRLAQQGKRVLRLKGGDPFIFGRGGEEIEDLVRHNIRFQVIPGITAAAGCASYAGIPLTHRDHAQSCLFVTGHLKDGSVDLNWNALCQPRQTVVVYMGLTGLEVICREMIRHGRERSTPAALIQQGTTLNQQVITATLESLPDAVAAAQVEAPTLLIVGSVVTLH",
    "response#3": "RIDFKEGKLSPEWIHLQNPEAKNYIFTKDGKLRLIATPVTLSDWKSPTFVALRQEHFDMEASAPVVLQKAGVNDEAGISVFMEFHSHYDLFVRQDKDRKRSVGLRYKLGEITHYAKEVSLPTDGEVELVVKSDINYYYFGYKVNGIYHDLGKMNTRYLSTETAGGFTGVVLGLYITSASKDSKAYADFEYFKYI"
  },
  {
    "reference": "MTEIGTFSGRHVLAEFDGIDARLLDDAAFLQETLRSVVTEAGATVCQVVTHRFEPHGVTALALLAESHASLHTYPELGTAFADVFTCGERADPELAVRLLAEALKAGGVRSSTTTRGRGSRPVSEPVGTGLTRSWDVSEVIIETRTDFQNLLIGRTAQGVSLFCDGERQSTEATQLTYHEALMVPAMLLADRIRRVLIIGSSEGVASQLAVAAGAEVVDHVDIDRVTVRACAENLPYGYTPEELAAAERGEGPVRVHYRDGWEFLAQAEPGYDVVVIDLPDENDDPEAQHNRLYGTEFLQRCAALLAPGGVVSCQAGCPTMWRNDTLITAWRRFKEAFGTVLYYGSDEHEWAFLSARADDAKPDLAVKLAALPYRPETIDADALRGNTVPPRLVRASGLPGRGA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must have a Schiff-base intermediate with substrate; via pyruvic acid active site that is highly conserved among related enzymes.2. The stability of the protein-pyruvate complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "SVWFTDRNENMALSIRHKGDCLFREKSPYQTVEVFDTFEYGKLLAVDKMVMCSEKDEKGYHEMIIHVPMVMQPEVKDVLVVGGGDGGSIREILRYDPVERVTMVEIDDAVVRASKEFLPSLSSAFDHPKLNLIIDDGIEYVKQAPDACFDMIVVDSSDPVGPAEGLFSKAFYQDVYRCLRPGGVMTAQSESPHFNQKAFVELNHCLKDIFNVDSVFCYLAFIPTYPTGMWSFTYCTNGWDRAYLKATLKGNHNILYNGSSPFQELLLIETMDIRLYLNQELQFSSLDERYYHEALVFPAMELAASPKRVLVLGGGDGLAVREILKYPNVEHVDLVDIDPKMVQLAKTHPALLVQNERSFLDEKVTVHIEDAKGYISGQLEPYDVIIIDFPDPSDSIISSLYTKELFSKVALLLAEDGVLVCQSNSPEEAPRLFWSIGKTIEAAGFYTRGYKILVPSFGVWGFHLATTEHDTSMFDG",
    "response#2": "NGWDRAYLKATLKGNHNILYNGSSPFQELLLIETMDIRLYLNQELQFSSLDERYYHEALVFPAMELAASPKRVLVLGGGDGLAVREILKYPNVEHVDLVDIDPKMVQLAKTHPALLVQNERSFLDEKVTVHIEDAKGYISGQLEPYDVIIIDFPDPSDSIISSLYTKELFSKVALLLAEDGVLVCQSNSPEEAPRLFWSIGKTIEAAGFYTRGYKILVPSFGVWGFHLATNGWDRAYLKATLKGNHNILYNGSSPFQELLLIETMDIRLYLNQELQFSSLDERYYHEALVFPAMELAASPKRVLVLGGGDGLAVREILKYPNVEHVDLVDIDPKMVQLAKTHPALLVQNERSFLDEKVTVHIEDAKGYISGQLEPYDVIIIDFPDPSDSIISSLYTKELFSKVALLLAEDGVLVCQSNSPEEAPRLFWSIGKTIEAAGFYTRGYKILVPSFGVWGFHLATAWPAETPMWWPNYEDFPNSQIRKIIAKRLLESKQKIPHLYLQSDVVLDPLLAFRKELQENHGVKVSVNDIVIKAVAVALRNVRQANAFWDAEKGDIVMCDSVDISIAVATEKGLMTPIIKNADQKSISAISLEVKELAQKARSGKLAPHEFQGGTFSISNLGMYPVDNFCAIINPPQAGILAVGRGNKVVEPVIGLDGIEKPSVVTKMNVTLSADHRIFDGQVGASFMSELRSNFEDVRRLLY",
    "response#3": "SVWFTDRNENMALSIRHKGDCLFREKSPYQTVEVFDTFEYGKLLAVDKMVMCSEKDEKGYHEMIIHVPMVMQPEVKDVLVVGGGDGGSIREILRYDPVERVTMVEIDDAVVRASKEFLPSLSSAFDHPKLNLIIDDGIEYVKQAPDACFDMIVVDSSDPVGPAEGLFSKAFYQDVYRCLRPGGVMTAQSESPHFNQKAFVELNHCLKDIFNVDSVFCYLAFIPTYPTGMWSFTYCTH"
  },
  {
    "reference": "MTSTAAHFIAETLEAAGVDRVYGVAGDSLNGFTEALRARDKIQWVHMRHEEAAAFAAGAEAQLTGDLAVCAGSCGPGNLHLINGLFDAQRSGAPVLAIAAHIPSSEIGIDYFQATHPESLFKDCSHYVELVSHPSMLPQVLERAMRVAIARRGVAVVVISGDVALEKLDAKPPTWIAPAPPRVIPPADQVEAFADLLNGAQRVTLLCGIGCAGARPEVLALADALKAPIVHAMRGKESIEHDNPFDVGMTGFIGFSSGYAAMKDCDTLVILGSGFPYRQFYPNKAKVVQIDISPEALGNRSRLDLGLLGDVGETLRALLPRLEPKADRSFLDAALAHYAKAREGLDALAQIPHKPSQVHPQQVTRIVSELAAKDAVFTCDVGTPTVWAARYLRLDGQRRLLGSFNHGSMANALLQAIGAQSAFPGRQVVSLSGDGGFSMMMGDFISLSQLGLPVKVVVLNNGTLGFVEMEMKAGGFLDTAVDLQNPNFAAMAQAMGVKGVRIERAQDLEAGIAEAFAHDGPALVDVVTARQELVMPPKITLDEAHKFGVFALKAVLDGRAAELIDLAKVNLKR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The stability of the protein-thiamine diphosphate complex should be optimized to maximize enzymatic activity.2. The designed protein should be optimized for ubiquinone binding, flavin adenine dinucleotide binding activity in order to improve pyruvate catabolic process. The designed protein sequence is ",
    "response#1": "TQLASYLNDGKKVTLLCGAGCQGAHDEIMELCNKLKSPMVVALRGKEFLEYDNPYFAGLTGLIGYSSGYDAMMECDTLLMLGTDFPYRQFYPKNAKVLQVDIRSEHLGRRTPLTLGVVGDVKDTVEAR",
    "response#2": "TQLASYLNDGKKVTLLCGAGCQGAHDEIMELCNKLKSPMVVALRGKEFLEYDNPYFAGLTGLIGYSSGYDAMMECDTLLMLGTDFPYRQFYPKNAKVLQVDIRSEHLGRRTPLTLGVVGDVKDTVEAI",
    "response#3": "PIHPQQVAKAISDRAAKDAVFTCDVGLPTVWAARYLAMNGKRRLIGSFWHGSMANAMAQAIGAQAAFPKRQVISLSGDGGFAMLMGDLLSLTQLGLPAKIVVFNNGTLGFVEVEQKSTGFLPTGTGLTNPNFAAMAEAVGIRGIRLEDPGEVEHGIAAAFAHDGPVLVDAVVSRTELV"
  },
  {
    "reference": "MQTFNIAVLSGDGIGPEIIAEAIKVLDVVQQKYAFKLNYRTFDVGGIAIDNHGTPLPEATLKGCEESDAILFGSVGGPKWEHLPPAQQPERGALLPLRKHFALFCNLRPATLYKGLEKFCPLRADISAKGFDMVTVRELTGGIYFGQPKGREGEGANEKAFDTEVYHRYEIERIARVAFETAMKRSKHVTSVDKANVLISSVLWREVVCDVAKDYPEVTLDHIYIDNATMQLIKQPEFFDVLLCSNIFGDIISDECAMITGSMGMLPSASLNEKGFGLYEPAGGSAPDIAGKGIANPIAQILSAAMMLRHSFNLNDAATAIENAVKNVLAEGHRTADLADESQPLSTKQMGDLIAQAVE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Mn(2+), Mg(2+) is required for the proper folding and stability of the protein.2. Ideal protein should have a binding affinity for key enzymes in the Amino-acid biosynthesis, L-leucine from 3-methyl-2-oxobutanoate: step 3/4, L-leucine biosynthesis pathway to enhance the overall efficiency of the pathway.3. The ideal protein would be one that plays a critical role in leucine biosynthetic process through magnesium ion binding, NAD binding, 3-isopropylmalate dehydrogenase activity within cytoplasm.4. The protein have enhanced (2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate   + CO2 + NADH.5. The protein must exhibit the following characteristics: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate.6. The designed protein should have a binding site that is specific to the target Mg(2+), substrate, NAD(+) ligand. The designed protein sequence is ",
    "response#1": "QIAVLPGDGIGPEIVEQAERVLKALDLPLELRQAPVGGAAFDQFEHPLPPATLELAQGSHAVLFGAVGDWKYDTLPREFRPEQAILGLRKALGLFANLRPAILYPELASASSLKPEIVSGLDILIIRELTGDIYFGTPRGVRTAADGAFAGEREGYDTMRYAESEVRRIARIGFESARKRNKKLCSVDKANVLETSQFWRDLVIEVSRDYPDVELSHMYVDNAAMQLVRNPRQFDVIVTGNLFGDILSDEAAMLTGSIGMLPSASLNAAGQGLYEPSHGSAPDIAGQGIANPLATILSAAMLLRYSLNLAPQADRVEAAVRKVLADGLRTADIHEAGTTKVSTSQMGDAIAAAVG",
    "response#2": "MKIAVLPGDGIGTEIVQVGVAMLEAAARRFDFAFDFEEAPIGGAAIDATGEPLPAATLELCKASDAVFLGAVGGPQWDTLPSDKRPEKALLGLRAGLGLFANLRPARILPQLVAASALKPEVVEGVDILVVRELTGGLYFGIPKGIFPEKKGGRRGVNTMSYSAFEIERIGRVAFEAARERRGKLCSVDKANVLEVSQLWREVLVGLAPEYPDVELTHMYVDNCAMQLVRRPRQFDTIVTENMFGDILSDEAAMLTGSIGMLPSASLGSGGPGLYEPVHGSAPDIAGQDKANPLAQVLSGAMLLRHSLRQPEAAQAVERAVQTVLEQGYRTGDIAAPGAQIVGCRAMGEAIRGLV",
    "response#3": "TIVVLPGDHVGTEITEEATKVLNAIAEKRPNIKFDFQHHLIGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGDVRPEQGLLKIRKELGLYANLRPCNFAFDSLLDMSPLKPEYARGTDFTVVRELVCGIYFGKRKEDTGDGVTWDSEQYSVPEVQRITRMAASLALQHNPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLSVQHQLIDSAAMILVKNPTKLNGLVITSNMFGDIISDEASVIPGSLGLLPSASLASPPDTNKAFGLYEPCHGSAPDLPKGKVNPVATILSVAMMLKLSLDMVEEGIAVEKAVRKVIDNGIRTADLRGTNSTTEVGDAIAKAAEALLAGKAPAGLVTKAA"
  },
  {
    "reference": "MRDWEGMRNLPKALRAELAERYRVSEFAEVTPFVSQDGSVKYLYTLWDGKKTEAVYMPYVGRRTICVSSMVGCPAGCTFCATGKMGFGRNLTAAEILDQILFAAHHQGHSPREIRNVVLMGMGEPLLNLEHVFTAVRRMLDPEALAMSPRRITLSTVGIPRGIYRMAEEDLGVRLALSLHAPDDETRKLIIPTAHRYSLEEIMTAVRHYYARVKRRVTFEYTMLKGLNDHPWQAKALAKHLEGLSAHLNLIPWNPWEGAPHQGTPKEHILKFAAILEGMGVPTSIRWSRGRDVGAACGQLALRQPA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.2. The protein design must be able to perform rRNA (adenine-C2-)-methyltransferase activity in order to contribute to rRNA base methylation.3. The presence of [4Fe-4S] cluster is crucial for the protein to function optimally under specific conditions.4. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity.5. The S-adenosyl-L-methionine, [4Fe-4S] cluster binding site should be stable and able to withstand changes in environmental conditions.6. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. The designed protein sequence is ",
    "response#1": "MTACVSSQVGCSLTCKFCATGYMERKRNLNPDEIYDQVVLIHQQAVQNYGLPLSNIVYMGMGEPLLNYANVLKSIEKITSEEGLNMSPKRITVSTAGIAKMIRKLGDDGVKFNLALSLHAANDEKRNTIMPINEQNSLKALADALKYFYAKTKNPVTYEYIVFNDFNDNIEDAAELAKFCKHLPCKVNIIEYNPISFAAFTNADVDKIEAFAEYLEHLTEYYTENPRRHLLDLSLAELRDWVVERGEKPFRAAQVWRWIYQGRAADFESMTDLSRSLRQHLAEDFVLFAGSIARHQTSPDGTEKLLIGLADGGEVECVLLRDGPRRSICVSSQVGCAMGCVFCASGLDGVDRNLTRGEILEQMLRLQHRLDHEERLSHIVMMGMGEPLANLERVLSALDVARSPEGLGISPRRITISTVGLPPAIDRLARHGVPYNLAVSLHAPEDELRNRLVPVNRKIGVEALMAAADRYFEASGRRLTFEYVLLGGLNDSPTQAAQLGHLLRGRGAMLNVIPYNPVAGLPYRTPTSTATSRFREVLERGGINVNFRVRKGGEIDAACGQLRRNRQATERDNL",
    "response#2": "MTACVSSQVGCSLTCKFCATGYMERKRNLNPDEIYDQVVLIHQQAVQNYGLPLSNIVYMGMGEPLLNYANVLKSIEKITSEEGLNMSPKRITVSTAGIAKMIRKLGDDGVKFNLALSLHAANDEKRNTIMPINEQNSLKALADALKYFYAKTKNPVTYEYIVFNDFNDNIEDAAELAKFCKHLPCKVNIIEYNPISFAAFTNADVDKIEAFAEYLE",
    "response#3": "MTACVSSQVGCSLTCKFCATGYMERKRNLNPDEIYDQVVLIHQQAVQNYGLPLSNIVYMGMGEPLLNYANVLKSIEKITSEEGLNMSPKRITVSTAGIAKMIRKLGDDGVKFNLALSLHAANDEKRNTIMPINEQNSLKALADALKYFYAKTKNPVTYEYIVFNDFNDNIEDAAELAKFCKHLPCKVNIIEYNPISFAAFTNADVDKIEAFAEYLKGLFG"
  },
  {
    "reference": "MADAVFNTIESALEDIRNGKLVIVVDDEDRENEGDFITAAENVTPEMVNFITKEARGLLCVAVTASRALELQLDPMTQTNTSAHETNFTISVDARLAGVTTGISAFDRAITIRHLADPLAKPSDFARPGHVFPLRAMEGGVLRRVGHTEATVDLARLAGFRPVGVLCEILHDDGSMARVPDLLKLRDKFGMKLITIKDLVAYRMMREKLVRRAVEVNLPTHLGDFKLIAYETLIDDKNHLALVKGNVSNGDPVLLRVHSSCATGDIFGSMRCDCGEQLATAMSMIEREGRGVLLYIMQEGRGIGLINKLKAYNLQDDGMDTVEANETLGFKADLRDYGIGAQILLDLGIRKMRLMTNNPKKVVGLEGYGLEVTERVPLEIDANDINKDYLETKRDKMGHIMNGDRLARETMLKKIFGHR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must have a Nucleophile; for GTP cyclohydrolase activity, Proton acceptor; for GTP cyclohydrolase activity active site that is highly conserved among related enzymes.2. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity.3. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..4. The protein should have a high affinity for D-ribulose 5-phosphate, Zn(2+).5. The protein design must be focused on enhancing the efficiency of riboflavin biosynthetic process. The designed protein sequence is ",
    "response#1": "ASGRVPSEHGPFTAITYRSPADDCEHVALVLGEVNQAGAEPGSAESAPVLVRVHSECLTGDVFASRRCDCGEQLAVAMTRIGEAGRGVVVYLRGHEGRGIGLSHKLRAYNLQDAGLDTVDANLAQGLPVDSREYGIGAQILRDLGVREIRLMTNNPAKYRGLAGHGVRVATGRQDHGVMERVTQARIPTVSGAFKAIAYESHDGRTHMALVAGDPAGKEHVLVRVHSECFTGDVLGSTRCDCGVQLSEALERIQQEGEGVLVYIRGHEGRGIGLRHKVEAYALQDGGLDTVEANVELGFAPDTRDYGVGAQILRDLGITTMRLLTNNPTKRAGLEGYGLEIVERIASGRVPSEHGPFTAITYRSPADDCEHVALVLGEVNQAGAEPGSAESAPVLVRVHSECLTGDVFASRRCDCGEQLAVAMTRIGEAGRGVVVYLRGHEGRGIGLSHKLRAYNLQDAGLDTVDANLAQGLPVDSREYGIGAQILRDLGVREIRLMTNNPAKYRGLAGHGVRVATGRGDHGRVAQTMLPTEFGEFTAYGFRDEIDASEHVALVHGDIGGGRDVLTRLHSECLTGDVLGSQRCDCGPQLQASMARITEEGAGVVVYLRGHEGRGIGLLAKLQAYALQDAGRDTVDANLDLGYAADSRDYSAGAQMLRDLGIVSVRLLSNNPAKERALETYGVSVSERERVTQARIPTVSGAFKAIAYESHDGRTHMALVAGDPAGKEHVLVRVHSECFTGDVLGSTRCDCGVQLSEALERIQQEGEGVLVYIRGHEGRGIGLRHKVEAYALQDGGLDTVEANVELGFAPDTRDYGVGAQILRDLGITTMRLLTNNPTKRAGLEGYGLEIVERI",
    "response#2": "RMVSTVLPTRNGRFDVHLYRSAVDTKEHIALVKGTIDAGAPVLVRVHSECLTGDIFGSQRCDCNEQLHTSMEMIEREGNGIVLYMRQEGRGIGLLNKLRAYKLQDEGKDTVEANEALGFHADVRDYSLAAQILLDLGVRRVRLLTNNPKKIVGLKGFGIEVTERIH",
    "response#3": "GVDMPTQWGDFSLIAYTQVATGDTHLALVKGEWAPGEPVLTRVHSSCVTGDIFGSCRCDCGGQLHRAMTMVEEAGKGVVLYMFQEGRGIGLLNKLKAYKLQEMGRDTVEANLELGFKMDERDYGVGAQILRDLGVSKLRLMSNNPKKRNALAGYGLEITETVGG"
  },
  {
    "reference": "MAKKSMIERDKKRKFLSAKYSIQRQQLKEQINQSLSLDEKAHLYRKLQSLPRNSAPTRITRRCFVTGRPKAVYRDFGLSRHVLREMAHACLLPGVIKASW",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be optimized for chloroplast-specific folding and stability, while maintaining structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "NVLNRFFLMNRSDRNFEYGIQRDQIGKDTLNHRTRMKYMINQHLSNLKKSQKRWFDPLILISRTERSTNRDPDAYRYKWSNGSNNFQEHLDHFVSERKSRFRVVFDRLRINQYSIDWSEVIDKKGLSKPFRFFLSKSLLFLSKSLLFLSKFLFFLSNSLPFFFVSFGNIPIHRSEIYIYELKGPNDQLCNQLLESIGLQIVHLKKLKPFLLDDHDTSQKSKFLINGGTISPFLFNKIPKWMIYSFHTRNNRRKR",
    "response#2": "NVLNRFFLMNRSDRNFEYGIQRDQIGKDTLNHRTRMKYMINQHLSNLKKSQKRWFDPLILISRTERSTNRDPDAYRYKWSNGSNNFQEHLDHFVSERKSRFRVVFDRLRINQYSIDWSEVIDKKGLSKPFRFFLSKSLLFLSKSLLFLSKFLFFLSNSLPFFFVSFGNIPIHRSEIYIYELKGPNDQLCNQLLESIGLQIVHLKKLKPFLLDDHDTSQKSKFLINGGTISPFLFNKIPKWMIYSFHTRNNRRKR",
    "response#3": "DSSQLKGSSDQSRDPLDSISNEDSEYHTLINQRKIQQLKERSILWDPSFLQTERTEIESDRFPKSLSGYSSMSRLFTEREKQVINHLLPEEIEEFLGNPTRSVRSFFSDRWSELHLGSNPTERSTRDHKLLKKQQDLSFVPSRRSEKKEMVNIFKIITYLQNTVSIHPISSYPGCDMVPKDEPDMDSSNKISFLNKNPFLDLFHLFHDRNMGGYTLHHDFESEERFQEMADLFTLSITEPDLVYHKGFAFSIDSYGLDQKQFLNEVFNSRDESKKKSLLALPPFFYEENESFYRRIIKKWVRISCGNDLEDPKPKKMVFASNNF"
  },
  {
    "reference": "MELRKDKLLMFYSEGKESKEAKWAVNDPMSKSYKLSLPSALRPDNLLPGNRLRYTDASKSKSSKVSWYKTILDPGSEIVLKWNWVFIVSCMVALFIDPLYFFVPAIGGDKNYPCARTDTSLSILVTFFRTIADLFYLLHIFIKFRTGFIAPNSSTRVFGRGELVMDPKAIAWRYIKSDFIIDLIATLPLPQIVIWFVISTTKSYRFDHNNNAIALIVLLQYIPRFYLIIPLSSQIVKATGVVTKTAWAGAAYNLLLYMLASHVLGAAWYILSVDRYTSCWKSRCNGEAGQVNCQLYYLDCDSMYDNNQMTWANVTKVFKLCDARNGEFKYGIFGNAITKNVVSSQFFERYFYCLWWGLQQLSSYGQNLSTTMFMGETTFAVLIAIFGLVLFAHLIGNMQTYLQSLTVRLEEWRLKKRDTEEWMRHRQLPEELRNRVRRYEQYKWLATRGVDEEVLLQSLPTDLRRDIQRHLCLDLVRRVPFFSQMDDQLLDAICERLVSSLCTEGTYLVREGDLISEMLFIIRGRLESSTTNGGRTGFFNSIILRPGDFCGEELLSWALLPKSTLNLPSSTRTVRALVEVEAFALRAEDLKFVANQFRRLHSKKLQHTFRFYSHHWRTWAACFIQAAWRRYKRRVMENNLTAIESMENEEGEVGEELVVVEEEECVEESPRTKMNLGVMVLASRFAANTRRGVAAQRVKDVELPRFKKPEEPDFSAEHDD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a binding site that is specific to the target a nucleoside 3',5'-cyclic phosphate ligand.2. The Extracellular, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.3. For general function, the protein need meet that Forms a functional cation-translocating unit with AHAs that is activated by PSKR1/BAK1 and possibly other BAK1/RLK complexes; Required for PSK-induced protoplast expansion.; Probable cyclic nucleotide-gated ion channel4. The designed protein must be optimized for calmodulin binding, cAMP binding.5. The designed protein should have at least Helical; Name=H5, Helical; Name=H6, Helical; Name=H2, Helical; Name=H3, Helical; Name=H4 transmembrane domains to ensure proper localization.6. The presence of IQ domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "LDCGLASNESPYNEANSNLTYISDADFIQGGKTGNVQKDLLMKLRKPYTVLRYFPDGIRNCYSLNVKQDTNYLIRVMFRYGNYDGLNNSPRFDLYLGPNIWTTIDMGKSGDGVLEEIIHITRSNILDICLVKTGTSTPMISSIELRPLLYDTYIAQTGSLRNYNRFYFTDSNNYIRYPQDVHDRIWVPLILPEWTHINTSHHVIDSIDGYDPPQDVLRTGAMPANASDPMTITWNLKTATDQVYGYIYIAEIMEVQANETREFEVVVNNKVHFDPFRPTRFEAQVMFNNVPLTCEGGFCRLQLIKTPKSTLPPLMNAFEIFRVFSKDEKIIREGDPVQRMIFIMRGRVKRIQSLSKGVLATSTLEPGGYLGDELLSWCLRRPFLDRLPPSSATFVCLENIEAFSLGSEDLRYYKRVSIHEHLLNGTGTALNGSSGKLVILPDSMQDLMKLSEKKFGKAARGILTVGGAEVEDIEVIRDGDHLFRIAHRDLKSKNILLFQGVSDKLVLPLVAEMKAESFPPKADIILENEASTDCYIIVSGEVEVLTTLEDGTEKQVMRIGPRGMAGEIGVMFNIPQPFTIRSRKLTQLVRISHSHMVSTIRPNTADGVVVFSNFIEESESHRVSIHEHLLNGTGTALNGSSGKLVILPDSMQDLMKLSEKKFGKAARGILTVGGAEVEDIEVIRDGDHLFFSPLFQGVSDKLVLPLVAEMKAESFPPKADIILENEASTDCYIIVSGEVEVLTTLEDGTEKQVMRIGPRGMAGEIGVMFNIPQPFTIRSRKLTQLVRISHSHMVSTIRPNTADGVVVFSNF",
    "response#2": "LDCGLASNESPYNEANSNLTYISDADFIQGGKTGNVQKDLLMKLRKPYTVLRYFPDGIRNCYSLNVKQDTNYLIRVMFRYGNYDGLNNSPRFDLYLGPNIWTTIDMGKSGDGVLEEIIHITRSNILDICLVKTGTSTPMISSIELRPLLYDTYIAQTGSLRNYNRFYFTDSNNYIRYPQDVHDRIWVPLILPEWTHINTSHHVIDSIDGYDPPQDVLRTGAMPANASDPMTITWNLKTATDQVYGYIYIAEIMEVQANETREFEVVVNNKVHFDPFRPTRFEAQVMFNNVPLTCEGGFCRLQLIKTPKSTLPPLMNAFEIFDFPIVYCNESFCKISGYNRAEVMQKSCRYVCGFMYGELTDKETVGRLEYTLENQQQDQFEILLYKKNNLQCGCALSQFGKAQTQETPLWLLLQVAPIRNERDLVVLFLLTFRDENVKRVSIHEHLLNGTGTALNGSSGKLVILPDSMQDLMKLSEKKFGKAARGILTVGGAEVEDIEVIRDGDHLFESFPPKADIILENEASTDCYIIVSGEVEVLTTLEDGTEKQVMRIGPRGMAGEIGVMFNIPQPFTIRSRKLTQLVRISHSHMVSGLCELFQGVSDKLVLPLVAEMKAESFPPKADIILENEASTDCYIIVSGEVEVLTTLEDGTEKQVMRIGPRGMAGEIGVMFNIPQPFTIRSRKLTQLVRISHSHMVSTIRPNTADGVVVFSNFVLPGDLLYHTGESIDSLCFIVTGSLEVIQDDEVVAILGKGDVFGDQFWKDSAVGQSAANVRALTYCDLHAIKRDKLLEV",
    "response#3": "LDCGLASNESPYNEANSNLTYISDADFIQGGKTGNVQKDLLMKLRKPYTVLRYFPDGIRNCYSLNVKQDTNYLIRVMFRYGNYDGLNNSPRFDLYLGPNIWTTIDMGKSGDGVLEEIIHITRSNILDICLVKTGTSTPMISSIELRPLLYDTYIAQTGSLRNYNRFYFTDSNNYIRYPQDVHDRIWVPLILPEWTHINTSHHVIDSIDGYDPPQDVLRTGAMPANASDPMTITWNLKTATDQVYGYIYIAEIMEVQANETREFEVVVNNKVHFDPFRPTRFEAQVMFNNVPLTCEGGFCRLQLIKTPKSTLPPLMNAFEIFYTEESYIVREGDPVDEMLFIMRGKLLTITTNGGRTGFLNSEYLGAGDFCGEELLTWALDPHSSSNLPISTRTVRALMEVEAFALKADDLKFRVSIHEHLLNGTGTALNGSSGKLVILPDSMQDLMKLSEKKFGKAARGILTVGGAEVEDIEVIRDGDHLFFSKRITLVECVGKGRYGEVWRGQWQGENVAVKIFSSRDEKSWFRETEIYNTVLLRHENILGFMASDMTSRNSSTQLWLITHYHENGSLYDYLQRVAVETVDGLLMATSVVSGLVHLHTEIFGTEGKPAIAHRDLKSKNILVKKDLRCCIADLGLAVTHSQTDNQLDIGNNPKVGTKRYMAPEVLDESIQTDCFDAYKRVDIWAFGLVLWEIARRTYSNGIVEEYKPPFYDLVPNDPSFEDMRKVVCVEHQRPFIPNRWFSDPTLSALAKLMKECWYQNPSARLTALRIKKTLDKIHSS"
  },
  {
    "reference": "MKLEELVKPLITTNALTDIPMDLDIQQVVQDTRQVTGQTLFICIRGFNVNGHDVVQEAVEKGAVAVIASEPIQVSVPVIYVKDTVKAMAQVGGTFYQHPSRKMKMIGVTGTNGKTTTTLLLDQIFRDHHEKTGVIGTMYRRIVDQVLETKNTTPDILTTQKTLDEMISAGVSTCSMEVSSHALVQGRVWGLDFDVAIFTNLSQDHLEYHHTMHEYAEAKALLFSQLGNSYDPSKPKYAVINADDPYGRSFFEKIAAPILTYSMEQSADFKAENLQIGKRGTGFTLVFQGEEYKVQMNLVGKFNVMNALAAIAGAYAAGMPLEDILTSIEKVSGVRGRFELVPADVDFSVIVDYAHTPDGLENVLNAANEIKSKRVICVVGCGGDRDPSKRSVMADTSMSLADVSIFTSDNPRTEDPDAILDQMTGHLESGTYTRITDRRKAIQEAVQKAETGDIVLIAGKGHEDYQIIGTTKHHFDDVEEAQAAIKAIKE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should contain one or more Mur ligase N-terminal catalytic, Mur ligase C-terminal domains that are essential for its function.2. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..3. The designed protein should have a high specificity for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding, with minimal off-target effects.4. The protein design should include a Meso-diaminopimelate recognition motif motif to ensure proper function.5. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.6. The protein should be optimized for activity in peptidoglycan biosynthesis, Cell wall biogenesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.7. A protein with UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity functions is required to enable cell cycle, peptidoglycan biosynthetic process, cell division. The designed protein sequence is ",
    "response#1": "MKLKELLEKIRIIKVEGDMERNIEFNSKKTAMNDVFVAIKGTNVDGHDFIEMAVKQGALAVVCERMPEKRKDRVTYILTDDTTKALSIMASNYYHQPSQKMKVIGVTGTNGKTTIATLLFKLFSQLNYKCGLISTVQNQIAGKILPSTHTTPDAISLNKLIAEMYAEGCTYVFMEVSSHAIDQHRIAGLTFAGGIFSNITHDHLDYHKTFDEYIRVKKQFFDSLPSFAFAISNADDKRGAVMLQNTKAKKYLYSLKTMADFKGKIIENSLRGLVLVINDIEVHCRLIGTFNAYNLLAVYGAAGLALEIDKETILTSLSHLENVKGRFDYITSPNGVIGIVDYAHTPDAVQNVLSTVQDIRKGIEQVITIIGCGGDRDKTKRPVMAQVASDWSDKVILTSDNPRSEHPETIIRDMEAGVTPVNKRKVLSITDRKEAIRTACHLARPGDIIVLAGKGHETYQEIKGVKFHFDDREVARKALESMQ",
    "response#2": "MRSGLSVDSREVREGHLFAALPGSTAHGAGFVAMAIEKGAAAILTDAEGARIAATALDGASVALVLSEEPRQALARAAVTGISVDSRQVQPGTLFAAIPGSKVHGAEFIQYALRMGAVAVLTDAEGATLAADALAQADVALVVTDAPREALSRAAALWYGRQPEVMVAIGVTGTNGKTTTSHLIEQILQAAGHRCGLIGTIETRIGDRVIPAQRTTPEALDLQRLLREMADAGCRAVAMEVSSHALDQKRVDGCRFRAAAFTNLSRDHLDYHGTMAAYAAAKAKLFDGLADGAVAVVNADDPAVVAMAAATPARVLTFGTDGDVVATHVTLDSRGVHGRIVTPEGELDVRVPLIGRFNVYNALAAIGAALVLGVPLSDALAGVAGCPGVP",
    "response#3": "MRLADLLAEIPLTAVAGDPAVTGISVDSRQVQPGTLFAAIPGSKVHGAEFIQYALRMGAVAVLTDAEGATLAADALAQADVALVVTDAPREALSRAAALWYGRPSDRLGLIGVTGTNGKTTTSHLIEQILQAAGHVAAIGTLGTGIPGWLEATHTTPDALELQRLFARMADAGAAAVAMEVSSHALAQGRVDGVAFAGAVFTNLSQDHLDFHGSMEAYGAAKARLFVGLDAVRVVNADDEYGRRLVAALPAEVVTYGIGRAGDVRARNVVLGAKGVRFEVGGAVGDFNVEMPLIGRFNVENVLAATAAALALGVPAGEVAAAAGRLRAVAGRMQLVTGHPSGARVIVDYAHTPDALRAALSALRPEAKGRLAVLFGAGGDRDPGKRSMMGKVAQNGADLVYVTDDNPRSEDPGDIRKAILDGCPDAVEIADRGAAITAALGELSAEDVLLIAGKGHEDYQIVGGEKRHFDDREELAAAIAARAADAARG"
  },
  {
    "reference": "MAGGVVVNNGGGKDYPGKLTMFVLFACIVAATGGLIFGYDIGISGGVTSMNPFLIKFFPSVYRKEQAAEKNQSNQYCKFDSPLLTMFTSSLYLAALVASFFASTVTRVAGRKWSMFGGGVTFLVGAALNGAAKNVLMLILGRVLLGVGVGFANQSVPLYLSEMAPARLRGMLNIGFQLMITIGILCANLINYGTAKIKGGWGWRVSLALAAVPAAIIAVGALFLPDTPNSLIDRGHTDAAKRMLRRVRGTDDIEEEYNDLVAASEESKLVAHPWRNILQRRYRPQLTMAIAIPLFQQLTGINVIMFYAPVLFKTLGFADDASLMSAVITGLVNVFATFVSIVTVDRLGRRKLFLQGGTQMLACQIVVGSLIGAKFGFSGVADIPKAYAAFVVLFICAYVAGFAWSWGPLGWLVPSEIFPLEIRSAGQSINVSVNMLFTFIIAQAFLPMLCRFKFILFFFFGAWVVIMTLFVAFFLPETKNVPIEEMVLVWKSHWYWGRFIRDEDVHVGADVEMPAAGNRNGKVDPAKLAN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Can transport glucose, fructose, mannose, galactose, xylose and ribose.2. The designed protein localizes to the plasma membrane. The designed protein sequence is ",
    "response#1": "WQKDRDIFVNKAESMGAKVFVQSANGDDSAQISQIENMINKNIDVLVIIPHNGEVLSNVISEAKKEGIKVLAYDRLINNADLDFYVSFDNEKVGELQAKSIVAVKPEGNYFLMGGSPVDNNAKLFRKGQMKVLDPLIASGKIKVVGDQWVDSWLAEKALQIMENALTANKNNVDAVVASNDATAGGAIQALSAQGLSGKVAISGQDADLAAIKRIVNGSQTMTVYKPITKLADKAAEIAVELGKC",
    "response#2": "WQKDRDIFVNKAESMGAKVFVQSANGDDSAQISQIENMINKNIDVLVIIPHNGEVLSNVISEAKKEGIKVLAYDRLINNADLDFYVSFDNEKVGELQAKSIVAVKPEGNYFLMGGSPVDNNAKLFRKGQMKVLDPLIASGKIKVVGDQWVDSWLAEKALQIMENALTANKNNVDAVVASNDATAGGAIQALSAQGLSGKVAISGQDADLAAIKRIVNGSQTMTVYKPITKLADKAAEIAVELGKCG",
    "response#3": "WQKDRDIFVNKAESMGAKVFVQSANGDDSAQISQIENMINKNIDVLVIIPHNGEVLSNVISEAKKEGIKVLAYDRLINNADLDFYVSFDNEKVGELQAKSIVAVKPEGNYFLMGGSPVDNNAKLFRKGQMKVLDPLIASGKIKVVGDQWVDSWLAEKALQIMENALTANKNNVDAVVASNDATAGGAIQALSAQGLSGKVAISGQDADLAAIKRIVNGSQTMTVYKPITKLADKAAEIAVELGKC"
  },
  {
    "reference": "MEFCENGTSWSDIWVDDGFNRCFYDAVSAIILFGFIFISGFIQCLVFSKYAVPAEQQGKLRWVSKVQVFLIIILILEALLHMVLSDVVINEQTLYGYQVMQACAVIAACIVAWWLLAMEKRSMLPSFSTRGQGFVSILFWMIFFVRENVMFVSWSSPAWWWQVKNDSDRAELGLFIVRYIAVFFLLALSFLRPIQSPYSSLPSTDEEPSNPAEQGKSTWSNSFTKVRMMLPYVWPKTSFILQFVVGICLVLLVVGRVINVFVPIYSKNIVNSLTYDPNVENQQLEFKWNYILIYVALLFLKGGTGTAGFVNNLRSLLWIRVEQYCTRSIQLRLFTHLHNLSLRWHIGKKTGEMLRVVDRGTASVTTLLSYILFQILPTIADIVIAIIYFVTAFNYLFGIITFLSMAMYLAATVKATEWRTKYRRQRNVRDNAANARAVDTLLNFETVKYYAATDYEREHYEKAILEYQLSEWKFNAVFNVLSLFQNIINTVSLIAGSMLCAWAVVHGIGGLKLNVGDYVLFGTYTSQLYGPLNTLGNTYRMIQQAFIDMENMFELLETEIEVKDVPGAKDLVVKGGAIEFENVSFQYEPNKPILKDVSFSVPAGHTFALVGHTGSGKSTIVRLLFRFYDVNSGCIKIDGQDIAKVTQKSLRSVIGVVPQDTVLFNNDIRYNIRYGRLDATDEEVEDAAKAAEIHERILTFPKKYDTVVGERGLRLSGGEKQRVAIARTLLKAPAIVLLDEATSALDTRTERSIQSSLARVCENRTTII",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein's functionality will benefit from Basic and acidic residues, Polar residues compositional bias in its structure.2. The protein should have ATP binding, ABC-type transporter activity and be active in early endosome membrane, melanosome membrane, extracellular region.3. The protein should have a Helical domain that is long enough to span the entire membrane.4. The designed protein must have at least one ABC transmembrane type-1 domain. The designed protein sequence is ",
    "response#1": "VTGSMKPGRITAVMGPSGAGKTSLLSALAGKAVGCKLSGLILINGKQESIHSYKKIIGFVPQDDVVHGNLTVEENLWFHAKCRLPADLSKADKVLVVERIIDSLGLQAVRSSLVGTVEKRGISGGQRKRVNVGLEMVMEPSVLFLDEPTQ",
    "response#2": "PGRITAVMGPSGAGKTSLLSALAGKAVGCKLSGLILINGKQESIHSYKKIIGFVPQDDVVHGNLTVEENLWFHAKCRLPADLSKADKVLVVERIIDSLGLQAVRSSLVGTVEKRGISGGQRKRVNVGLEMVMEPSVLFLDEPTSGLDSASSQLLLRALRHEALEGVNICMVVHQPSYTLFKTFNDLVLLAKGGLTVYHGSVL",
    "response#3": "PGQTVALVSSWTLGAGKSTIIRLLFRFYNIQGGCIRIDGQDISKVKQRSLRAHIGVVPQDTVLFNDNIRDNIRYGRISASDQEVEEAAIAADIHDKIMAFPEAYDTQVGERGLKLSGGEKQRVAIARTILKAPQIILLDEATSALDTQTERNIQASLAKVCANRTTIVVAHRLSTIIGADQILVISNAG"
  },
  {
    "reference": "MDNILNPEDNVSQTNTETDVTDGPFQYVKEEHSSHKFTASGQNLDSPPKNKKSPLKRKAGEPESPSKRPRLEEGHGSLVVTHYNELPETGLETRSQSRIFHLRNFNNWMKSALIGEFVEKVRQRTRNIAVLDLGCGKGGDLLKWRKGGISKLVCTDIADVSVKQCEERYKDLKRKSRNERVFEAEFLTADSTKELLSEKYNDPEIKFDICSCQFVYHYSFETYEQADMMLRNACERLCPGGFFIGTTPDGFELVKRLEASDTNSFGNDVYTVKFEKKGKYPLFGCKYDFSLEEVVNVPEFLVYFPVLVEMAKKYQMKLIYKKTFREFFEEKVKNDEQKMLLKRMKALEPYPAAPNFKLVSGRTEDYEHAQKLVENGQVKLPLGTLSKSEWEATSIYLLFAFEKQA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must have high specificity towards mRNA (guanine-N7-)-methyltransferase activity, RNA binding.2. The protein should be designed to catalyze the following reaction a 5'-end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA +   S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-   triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-   homocysteine.3. The protein must exhibit the following characteristics: Binds RNA containing 5'- terminal GpppC..4. The protein design should emphasize Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "MIFGIHAVKAVLATDPVRLIEVFVLKDRQDDRLMPLLTELKDLGITIQHAGRKALDDKAKGASHQGIIARVKPAKRS",
    "response#2": "MRASQSRGVADVVRQHYNAVPQRGREWRKTESKIKGLRSFNNWVKSTLIQKFSPDEEFLARFNGTKEWAEDGGVPPVEEKRLLVVDLGCGKGGDLGKWQLAPQPVDLYVGLDPAEVSIVQARERYNGMKSGRGNRGRRNPIFHAEFRPKDCFGEWLGDVDIVQQVGIDPNVGPGGSVMSSRWGGGGFDVVASMFTIHYAFESEEKARQMLRNVAGCLKKGGRFLGVCPNSDVISARVSEINAKKKARQAQAKKEKSDEAPEDGEVEEDDGKVEWGNQIYRVRFPITPPEDGVFRPPFGWKYSYFMEEAVEEVPEYVVPWEAFRALTEDYNLELQYRKPFLDIWRDEKDDPELGPLSERMGVRDRVTGKLLMTEEEKEAASFYHAFCFYKQ",
    "response#3": "MRASQSRGVADVVRQHYNAVPQRGREWRKTESKIKGLRSFNNWVKSTLIQKFSPDEEFLARFNGTKEWAEDGGVPPVEEKRLLVVDLGCGKGGDLGKWQLAPQPVDLYVGLDPAEVSIVQARERYNGMKSGRGNRGRRNPIFHAEFRPKDCFGEWLGDVDIVQQVGIDPNVGPGGSVMSSRWGGGGFDVVASMFTIHYAFESEEKARQMLRNVAGCLKKGGRFLGVCPNSDVISARVSEINAKKKARQAQAKKEKSDEAPEDGEVEEDDGKVEWGNQIYRVRFPITPPEDGVFRPPFGWKYSYFMEEAVEEVPEYVVPWEAFRALTEDYNLELQYRKPFLDIWRDEKDDPELGPLSERMGVRDRVTGKLLMTEEEKEAASFYHAFCFYKS"
  },
  {
    "reference": "MTANLLDFDLEGLAAFCEQLGEKRFRAVQLFRWIHQRGASDFTRMTDLARSLRDKLPAVCTITDLPVLSRQDSADGTIKWLFDVGGGNAVETVFIPEEDRGTLCISSQAGCAVGCRFCSTGHQGFSRNLTTGEILAQLWHAEFFLRKHLKRDERVITNVVMMGMGEPLQNYNALVPALRTMLDDHGYGLSRRRVTVSTSGVVPMIDRLAQDCPVALAVSLHAPNDALRDDLVPLNRKYPIAELLDACLRYLPAAPRDFITFEYCMLDGVNDQPEHAQELLALIRHHGARGVPCKINLIPFNPFPDSGLVCSPRAAVLRFGQILNEGGVVTTVRKTRGDDIDAACGQLAGDVLDRTNAAQRLAQRRQTTERPIRFVHKERQA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must bind to [4Fe-4S] cluster in order to perform its enzymatic function.2. The ideal protein would be one that plays a critical role in rRNA base methylation through rRNA (adenine-C2-)-methyltransferase activity, tRNA binding, rRNA binding within cytoplasm.3. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs; m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.4. The S-adenosyl-L-methionine, [4Fe-4S] cluster binding site should be stable and able to withstand changes in environmental conditions.5. The presence of Radical SAM core domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "YVYGYSICISTQIGCRMGCKFCASTIDGRVRDLTAGELIGEILAVQKDLGQRISNIVLMGSGEPLDNYENVIKFIKMVNEKYSLNIGQRHITLSTCGIVPKIYDLAREGLQITLAISLHAPNDIIRKQSMPVANSYSIDELIKSCQFYIDKTNRRVTFEYALIKGLNDSPSCAEELSKMLKGLLCHVNLIPINEVKEREYIKADKEIINEFKSILLKNGIEATLRREMGGIE",
    "response#2": "SSQAGCAVGCRFCSTGHQGFSRNLSTGEILAQLWFAEHFLRAHLETHGERVISNVVMMGMGEPLQNYAALVPALRVMLDDHGYGLSRRRVTVSTSGVVPMIDRLGEDCPVALAVSLHAPNDELRDKLVPLNRKYPIAELLDACNRYLEHAPRDFITFEYCMLDGVNDQPEHVHPYVRTTNLLDLDRRGLEGFFAAMGEKSFRAQQVMKWIYQLDVDDFAGMSNLSKALRERLHNAARMELPRVVMDRLSADGSRKLLLQLADGNCIETVFIPEKERGTLCVSSQVGCMLNCSFCSTAQQGFNRNLTTAEIIGQVLLASRALGGDVQYKRAITNVVMMGMGEPLLNYDAVVRAMNIMRDDLAFGLSKRRITLSTSGIVPELDRLRAECDVSLAVSLHATNDELRNELVPINRKYPIAELMEACRRYVADEPRRRVTFEYVMLAGVNDSDAHARELLRLLRRVPSKVNLIPFNPFPNTAYVSSDPQRILEFQNILINGGIVTVTRKTRGDDIAAACGQLAGEFRDRTRRREHH",
    "response#3": "EGLGEPSYRGGQILEWLWQKRATSIEGMSNLPVRLREHLEESFTLRSLSPAKSQGSRDSTRKFLFRLNDGRYIECVLIPASPALYGERSDRRTLCVSSQVGCAFGCKFCASGLDGFTRNLTADEIAGQVLLAEELSGEKVNNIVFMGMGEPLANLPQLLKALELITSPWGLGIGARHLTVSTSGLVPQIRKLADHPQQIRLAISLHGASNDVRDRIMPVNRKWPVEDLFKALSYWTKRKNQKITLEYILIDGINADPAQARLLAQHAESLRAKVNLIPYNTVDGLKWKRPPEEECHAFRDILAARGIPATLRLEKGHDIDAACGQLRLKEERAEKAG"
  },
  {
    "reference": "MPELPEVETVRRKLEKKIVGRQIIKVKVLEEKQFFGDPEAIIGRKIKSLWRRAKILVIDIDGLSLLVHLKMTGQIIYLSKGKGLVSVGHPLPFSTDQLPNKTTRIIIDLDDGGKIFFNDLRKFGWLKLLTQKEVKKELSKFGPEPLKKAFTQKYLANKLGQTKREVKTVLLDQSVIAGIGNIYANEVLFRAGINPRRQANSLSDREITKLYQAIRKILSKAIDYGGTSAADQAYVQPDASPGQYQEKLKVYQRDGQKCYRCGGIIKRFKQGSRSTFWCPGCQGTAKNSKTITEN",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein contains novel Formamidopyrimidine-DNA glycosylase catalytic domains that confer a unique function or activity.2. Zn(2+) is required for the proper folding and stability of the protein.3. The designed protein must possess class I DNA-(apurinic or apyrimidinic site) endonuclease activity, zinc ion binding to support translation under certain conditions.4. The protein must exhibit the following characteristics: Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG); Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents.5. The protein with the reaction Hydrolysis of DNA containing ring-opened 7-methylguanine   residues, releasing 2,6-diamino-4-hydroxy-5-(N-   methyl)formamidopyrimidine., the Schiff-base intermediate with DNA, Proton donor; for delta-elimination activity, Proton donor must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MPELPEVETVVRTLEKQLGNKTIKEVKVFWDNIIANVNPDVFASSLVNQTLEKYDRRGKFLIFTLTDYIVISHLRMEGKFFIQPLNTLKDKHTHLIFKMEDIELHYNDVRKFGPELPEVESIRRNLETVLKGETIKDVKVFYRPIVSNMMDFEEKLIGQTIKRIDREGKYLKFILDDYMMISHLRMEGKYFMDKDHDKHTHVEFYFESGHKLSYHDTRKFPELPEVETIRRSLSQHILERRIEEILIRWPGAVEGYEEKTFADAVRGLKFQSIERRGKYLLFTLEEGWSFIAHMRMTGRMVYHAQSQEPEKHTHVVLKLSSGEIHFTDTRKFGRLKVYGRGGEPCKECGHTLVRIRLAGRSTVFCPCCQR",
    "response#2": "MPELPEVELTRRRLERDITGKQVQQVLVRAPKLRLPVPPELEEALKGRTVRAVERRGKYLLLECEAGWLIVHLGMTGFLRLLHTPQLPGKHDHVDIVFTDGSVLRFHDPRKFGTVQFVDDDGLPQYVASLLSSQTQDPITYAAMQRLHQLGEGGGGLTIEAVLATKEERLDELLQPVGFHRRKAQQLKRVASMLRNQHHDDVPAGLDELLALPGIGPKIARVILLLAWGKVDGIVVDTHVHRLAQRLGWAGTTMGPESTRRELEDWIPELPEVESIRRNLETVLKGETIKDVKVFYRPIVSNMMDFEEKLIGQTIKRIDREGKYLKFILDDYMMISHLRMEGKYFMDKDHDKHTHVEFYFESGHKLSYHDTRKFGRFNVYGRAGQPCVQCDAILKADRHGQRSTAYCPQCQR",
    "response#3": "MPELPEVELTRRRLERDITGKQVQQVLVRAPKLRLPVPPELEEALKGRTVRAVERRGKYLLLECEAGWLIVHLGMTGFLRLLHTPQLPGKHDHVDIVFTDGSVLRFHDPRKFGTVTWRDGAPEPVLLSSQTQDPITYAAMQRLHQLGEGGGGLTIEAVLATKEERLDELLQPVGFHRRKAQQLKRVASMLRNQHHDDVPAGLDELLALPGIGPKIARVILLLAWGKVDGIVVDTHVHRLAQRLGWAGTTMGPESTRRELEDWIPRELWGKLSLAVVGFGQFQDAGDWRGVASLLSSQTQDPITYAAMQRLHQLGEGGGGLTIEAVLATKEERLDELLQPVGFHRRKAQQLKRVASMLRNQHHDDVPAGLDELLALPGIGPKIARVILLLAWGKVDGIVVDTHVHRLAQRLGWAGTTMGPESTRRELEDWIPELPEVETTRRKIEPLLRGKTIERIVHDAPHRYRNTERAHGRQVRGLTRRGKYLLLHLAAADAAEDEPHDLELIVHLGMTGGFRLEEGPHTRVTFELGSGEKLYFNDPRRFLAPGDLIAPELPEVETIKNNLQEILPLRIKELEIRREDILRCRDYALEELTGQIIEEASRRGKYLILAVDNGLFLVFHLGMSGRLYIQEEETTVLEPHVHVIIHLDKRLKLLYQDARRFLASGEL"
  },
  {
    "reference": "MSQISAKDVKNLRDITGAGMMDCKKALDETGGDMQQAIDYLRKKGAALAAKRADRDAREGMVEIKMTADHKAGIILELNCETDFVARGEDFTNFTVALGELALAKCISSPEALMTLTLGEAYGGETVDDAIKTMTGKLGEKISLKRLVYIDAGDGVVESYVHPGAQLGAIIHLATDQPDAVRELARDLAMQVAAAAPIVVDRSFVPADYIAKEAEIYRQQALEQGKKEAFVDKIVLGRLEKYYQDVVLSEQFFIKDSTVKVSDVLNEFRKKHQVKVDVIGFVRCKLGE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP; It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome..2. The protein can be targeted to cytoplasm and perform translation elongation factor activity. The designed protein sequence is ",
    "response#1": "MGAAMVELNCETDFVARNDIFGALARDIAHTAAWFPIVAASETSAVLSDVDVAAFLECPIIPFEASEENKHDVISVRAAITSVVARLGEKVALGRVASLAG",
    "response#2": "GNFLFTSESVSEGHPDKMAD",
    "response#3": "MPN"
  },
  {
    "reference": "MTAISPDTALYTSEQSRRIDARAIADAGIPGFELMSRAAAASFAMLRRRWPQASRIGIVCGAGNNGGDGYLVGRDALRAGLSVQLFALGEPRSEGDAARAREAFLAEGGLPQPWPGTLGNLDVLVDAIYGTGLNRAPQGDAQAAIEAISASGLPVLALDVPSGLSADTGATPGVAVRATATASFIVHKRGLHTGRCELAGDIELFDLGVPPDLRGTPDAVLLQPAAMPRRPRDSHKGNNGHVLAIGGDHGTAGAVRMAAEAALRTGAGLVSVATRDENVMPMNAARPELMAHGVNGLQALAPMLEKASVVALGPGLGQGAWGHALWTTALDAGLPTVLDADGLNLLHAEPRALPRHIVLTPHPGEAGRLLGVSTAEVQADRFASARELARRHAAVVVLKGNGSLVVAPDGRVSVCPWGNPGMASGGMGDTLTGIIAALLGQGCDPYEAACLGVGLHARAADVAAREGERGLLAGDLLTPLRRLVNGLEA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.2. A protein with metal ion binding functions is required to enable nicotinamide nucleotide metabolic process.3. The designed protein should be able to compete with endogenous proteins or inhibitors for (6S)-NADPHX binding.4. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.5. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MPALWQHFFPEMTHELHKYSRGHTVVFSGPSWATGAARLASYAAARIGSGAVTIIAPDEAVCALQNHLTSIMIYPENNNDDVMSFLSQRKVRSVILGPAFGDFNRAHDLIQFILLKHVVDHLVLDADAFTALAKKPQAFYNLIKESHVHVVMTPHEGEFQRLFPELASCHMTRIEKAKQAAKISGAVIVYKGADTIIASPCGRIAVTVNGSPHLATAGSGDVLSGLVGGLVAQNMPVFEAACAAVWFHAECGTYSGRGTIAEDLITVLPTVLKKFFP",
    "response#2": "MRDIEGRIFAAGMPVAALMEKVAGLITHRILELYPRSQAPCVGILVGPGHNGGDALVVARELHFQGYQIAVCSPFSKLKDLTSQHAQYVKNLKIPVHESLEPLAECDLFIDGLFGFGLGRSLFSPVDQIVNQLNEWSQPVVSIDLPSGLHTDTGEVLGTAVQATQTFCLGLWKLGLLQDQALDYVGAAELIDFDIPLADIEARKGDRGRVLVVGGGPYHGAPILAGLAAARMGADLIHVAMPTAALKRVKWPVDLIPEEIPDEEFLTGESVSGLVERMTTGRGVQALVIGPGSGRAEETLRAMRSLLTAAAEHGVPCVIDADAIYSLPRGEWPTGVIGVATPHGVEREGWLGEDDPAEIIAQAAESAQPSKWDLYSESAENAVIITTGVSDEIVGLGGRSSLASGGNPRMAVAGTGDLLAGSIGGLMACGMSPWAASRLACYLLREAGRVAGEQLGPGMVASDL",
    "response#3": "MMKLENAADQSGVTYDQMMENAGKGLAEHLVRIAVEMGVKEILFLCGNGNNAGDCFTAARLLTPQFKVTACLLAGVPKTRTAYAKYKMLKDIAVLEDQEAILEAVRRHRLIADGVFGIGFRGELSPFVQSVFAEIEADSEKKCIAVDIPSGGNGLSGDVAKGTPHCDMTITFGAAKTGLFLTPLSEYCGAIMLVDIGIPAPAFCVSGSRNMPGAAILASKAALRCGVGLFCLASDIDVCRLLIAQAPEATMLPLPTDYDGKLTQQAVPAILDYAKQATCVLIGCGLGQSEALRQAVTSLIPLLECPIILDADGLNAIAPSIDILHKAKSTVVLTPHPGEMARLLHCSVEEVQKDRMTAAKRLATRFPNTVVVLKGAGTIVATADHAHINTTGNSGMSKGGSGDILAGMIASLTAQHISPENAAIAGVYLHGLAGDCAAAENSRRAMLPSDMISQLL"
  },
  {
    "reference": "MHGPHPTVKDIELSLAPEDVPVQCNVQLDEEDYTNVEEPAQQAYRVVTLCPKCSSPLRLVVECSHADIRALEQLLLGTLTVVCPRCV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have a specific function within the host cell nucleus. The designed protein sequence is ",
    "response#1": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECV",
    "response#2": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECA",
    "response#3": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECK"
  },
  {
    "reference": "MKFVVKFFPEITIKSKPVRRRFVAQLHENLRAVLRELDPQVEVRRGWDKIEVVSDCEQASVETLVDAMRCIPGIYYILEVRDYPLPPVEEIADLVLPVYAERLRGKTFAVRCKRYGKHEFTSMDVERVVGGALRACADTAGVKLKDPEVQVELEISKKTLFVIGERHRGLGGYPVGSLDSVISLISGGFDSPVASYLAMRRGMRTHFLFFNLGGRDHELGVKEVALYLWQKYGCRQRVLFISVPFEEVVAELLTRVEDSQMGVILKRMMLRAADRIAADLEVDALVTGEAVAQVSSQTLRNLSVIDEVSERLVLRPLIATDKEDIVNLAREIGTGEFAANMPEYCGVISVNPTTRARLPRVQAEESRFDMAVLERAIGAARRTRIDRLADEALERTDVEVLPVPLAGSVIIDIRHPDEEEQAPLRLHVPVDRIPFYELHSRSGELDPAKTYMLYCGKGVMSRLHASHLVDAGFSHVKVYAPG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be designed to have high stability and activity towards [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 +   S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier   protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-   cysteinyl-[cysteine desulfurase].2. For general function, the protein need meet that This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway; Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor3. The designed protein should be involved in thiamine diphosphate biosynthetic process, tRNA thio-modification, thiazole biosynthetic process by localizing to cytoplasm and exhibiting tRNA U4 sulfurtransferase, tRNA binding.4. The presence of Rhodanese domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "IVKPHPEIFVKSESVRKRFIKILESNLRIIIKRRTESVAVYNRRDHIEVSASCDKYYEQVLEILTHTSGIHHTLEVLQTEFTDMHNIYEQVLERSGSKIVDKTFVVRAKRRGQHDFTSIELERYVGGGLNQAVESAKVKLKKPDVSINIEVENDKLNQVIERHKGLGGFPLGTQEDVLSLISGGFDSGVSSYLHIKRGSKTHFCFFNLGGPAHEIGVKQVAHYLWNKYGSSAKVKFIAVDFEPVVAEILENVDDGQMGVVLKRMFMRAGGMVAERFGIQALVTGEALGQVSSQTLTNLRHIDNVTDTLILRPLINWDKEDIIDLSRKIGTEDFAKVMPEYCGVISKKPTIKAEKSKLEAEEANFDFSILDGVIPEITIKSKPVRQRLVKQLRQNVQVVLSKETDGAKVQGFWDKIEVRLLPDYAADEPKLIAALQRIPGIANLVEDNAVVLDIRSAEEEEASPLELDSVEVKHLPFFKLATQFGDLDQSKTYLLYCDRGVMSRLQALYLQEQGFSNVKVS",
    "response#2": "VKPFAEITIKSRPVRRQFILQLGRNIRNVLKDLDPEIRTSGEWDNIDVVSSLTDPALQQQLVERMRHLPGIAHFYQVLDFDLPDSMQDIADRCAEHYAPLLKDKEFAVRCKRVGKHEFTSMDVEREVGSLLRQQCGAAGIRLNRPDVEVRLEIRHRRLLVEFNRQEGLGGYPLGSIEQALVLMSGGFDSTVAAYQLMRRGLLTHFCFFNLGGRAHELGVMEVAQFLWQKFGRSHRTLFISVPFEEVLGDILQNVDNSQMGVILKRQMLRAAEKVADNLHLDVLATGEAISQVSSQTLINLSVIDKATDKLVLRPLVAAHKQDIINTAEAIGTAEFAKHMPEYCGVISVNPTTKAKLGRIVHEEESLEALIDELQRIPGIHHVLEVEDRAYTDMHHIFEQTLELYRSRIEGKTFCVRVKRRGQHDFSSQDVERYVGGGLNQHVESASVKLNHPQETINLEIDNDRLMLVNGEALIDELQRIPGIHHVLEVEDRAYTDMHHIFEQTLELYRSRIEGKTFCVRVKRRGQHDFSSQDVERYVGGGLNQHVESASVKLNHPQETINLEIDNDRLMLVNGLHVGVDGLQEALIDELQRIPGIHHVLEVEDRAYTDMHHIFEQTLELYRSRIEGKTFCVRVKRRGQHDFSSQDVERYVGGGLNQHVESASVKLNHPQETINLEIDNDRLMLVNGLHVGVDGLQVEGPKLIAALQRIPGIANILKVESHELEGIDDRFEDIADICIKTYGDQVSGKSFVVRVKRAGQHSFASTDLERYLGGAFLQNTKASRVELREPDVAIKLEIRDQTLYAIAE",
    "response#3": "IVKPHPEIFVKSESVRKRFIKILESNLRIIIKRRTESVAVYNRRDHIEVSASCDKYYEQVLEILTHTSGIHHTLEVLQTEFTDMHNIYEQVLERSGSKIVDKTFVVRAKRRGQHDFTSIELERYVGGGLNQAVESAKVKLKKPDVSINIEVENDKLNQVIERHKGLGGFPLGTQEDVLSLISGGFDSGVSSYLHIKRGSKTHFCFFNLGGPAHEIGVKQVAHYLWNKYGSSAKVKFIAVDFEPVVAEILENVDDGQMGVVLKRMFMRAGGMVAERFGIQALVTGEALGQVSSQTLTNLRHIDNVTDTLILRPLINWDKEDIIDLSRKIGTEDFAKVMPEYCGVISKKPTIKAEKSKLEAEEANFDFSILDGVINIDEIVEELDQDLAVPVVDVVQPGQALIDIRHPDEEEANPLRVSGVDILKIPFYRLNNQFAQLDTGKQYFLYCQKGVMSQLHAANLKDAGHTNVGVYRIK"
  },
  {
    "reference": "MIPARFAGVLLALALILPGTLCAEGTRGRSSTARCSLFGSDFVNTFDGSMYSFAGYCSYLLAGGCQKRSFSIIGDFQNGKRVSLSVYLGEFFDIHLFVNGTVTQGDQRVSMPYASKGLYLETEAGYYKLSGEAYGFVARIDGSGNFQVLLSDRYFNKTCGLCGNFNIFAEDDFMTQEGTLTSDPYDFANSWALSSGEQWCERASPPSSSCNISSGEMQKGLWEQCQLLKSTSVFARCHPLVDPEPFVALCEKTLCECAGGLECACPALLEYARTCAQEGMVLYGWTDHSACSPVCPAGMEYRQCVSPCARTCQSLHINEMCQERCVDGCSCPEGQLLDEGLCVESTECPCVHSGKRYPPGTSLSRDCNTCICRNSQWICSNEECPGECLVTGQSHFKSFDNRYFTFSGICQYLLARDCQDHSFSIVIETVQCADDRDAVCTRSVTVRLPGLHNSLVKLKHGAGVAMDGQDVQLPLLKGDLRIQHTVTASVRLSYGEDLQMDWDGRGRLLVKLSPVYAGKTCGLCGNYNGNQGDDFLTPSGLAEPRVEDFGNAWKLHGDCQDLQKQHSDPCALNPRMTRFSEEACAVLTSPTFEACHRAVSPLPYLRNCRYDVCSCSDGRECLCGALASYAAACAGRGVRVAWREPGRCELNCPKGQVYLQCGTPCNLTCRSLSYPDEECNEACLEGCFCPPGLYMDERGDCVPKAQCPCYYDGEIFQPEDIFSDHHTMCYCEDGFMHCTMSGVPGSLLPDAVLSSPLSHRSKRSLSCR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should contain one or more VWFC, TIL, CTCK domains that are essential for its function.2. For general function, the protein need meet that Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.3. The protein design should include a Cell attachment site motif to ensure proper function.4. The designed protein should have protease binding, immunoglobulin binding within collagen-containing extracellular matrix, endoplasmic reticulum to regulate cell adhesion.5. The protein's architecture should predominantly feature strand-strand-helix-strand-strand-strand-strand-helix-strand-strand-strand-strand-strand-strand-turn. The designed protein sequence is ",
    "response#1": "PVDLLFVLDSSESIGLQNFQIAKDFIIKVIDRLSKDERVKFEPGESRVGVVQYSHNNTQELVAMGDANIDNIGALKQAVKNLKWIAGGTHTGEALQFSKENLLRRFTSNNNVAIVITDGRSDTLRDRTPLTSLCEVTPVVSLGIGDIFRNNPNPDQLLVDVVVVCDESNSIYPWEAVKNFLVKFVTGLDIGPKKTQVALIQYANEPRIIFNLNDFETKEDMVQATSETRQHGGDLTNTFRAIEFARDYAYSQTSGGRPGATKVMVVVTDGESHDGSKLKTVIQQCNDDEILRFGIAVLGYLNRNALDTKNLIKEIKAIASTPTERYFFNVADEAALLEKAGTLGEQII",
    "response#2": "LVDVVVVCDESNSIYPWEAVKNFLVKFVTGLDIGPKKTQVALIQYANEPRIIFNLNDFETKEDMVQATSETRQHGGDLTNTFRAIEFARDYAYSQTSGGRPGATKVMVVVTDGESHDGSKLKTVIQQCNDDEILRFGIAVLGYLNRNALDTKNLIKEIKAIASTPTERYFFNVADEAALLEKAGTLGEQIL",
    "response#3": "DIVIVLDGSNSIYPWDSVTAFLNDLLERMDIGPKQTQVGIVQYGENVTHEFNLNKYSSTEEVLVAAKKIVQRGGRQTMTALGIDTARKEAFTEARGARRGVKKVMVIVTDGESHDNHRLKKVIQDCEDENIQRFSIAILGSYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVTIVKTS"
  },
  {
    "reference": "MELITNELLYKTYKQKPAGVEEPVYDRTGNPLFGERGPIHPQSTLKLPHKRGKDEIPTNLASLPRKGDCRSGNSRGSVSGIYVKPGPLFYQDYIGPVYHRAPLEFFEEGSMCETTKRIGRTTGSDGKLYHIYVCIDGCILIKSATRYHQKVLRWVHNKLNCPLWVTSCSDTKEEGAKKKQQKPDRVEKGRLKITPKEAEKDSRTKPPDATIVVDGVKYQVKKKGKVKSKNTQDGLYHNKNKPQESRKKLEKALLAWAILAAVLFQVTMGENITQWNLQDNGTEGIQRAMFERGVNRSLHGIWPEKICTGVPSHLATDIELKRIHGMMDASEKTNYTCCRLQRHEWNKHGWCNWYNIEPWILLMNGTQANLTEGQPQRECAVTCRYDRSSDLNIVTQARDSPTPLTGCKKGKNFSFSGIVIQGPCNFEITASDVLFKEHDCTNVFHDTAHYLVDGMTNSLESARQGTAKLTTWLGKQLGILGKKLENKSKTWFGAYASSPYCDVERKLGYIWFTKNCTPACLPKNTKIVGPGKFDTNAEDGKIMHEMGGHLSEVLLLSLVVLSDFAPETASAMYLVLHFSIPQRHTDILDCDKSQLNLTVGVTTADVIPGSVWNLGKWVCIRPDWWPYETATALAFEEAGQVVRLTLRALRDLTRIWNAATTTAFLVCLVKVVRGQVVQGVIWLLLITGVQGHLDCKPDFSYAIAKDKRIGLLGAEGLTTTWYDYSDGMQISDTMVEVRCKGGEFTFVKKCKTETRYLATLHQRALPTS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have ribonucleoside triphosphate phosphatase activity, RNA binding, RNA-dependent RNA polymerase activity, ribonuclease T2 activity, serine-type exopeptidase activity within host cell cytoplasm, cytoplasm, host cell membrane, membrane to regulate suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity, viral protein processing, pore formation by virus in membrane of host cell, induction by virus of host autophagy, fusion of virus membrane with host endosome membrane.2. The protein must exhibit the following characteristics: Plays a role in the regulation of viral RNA replication..3. The designed protein is the optimization of the Charge relay system; for serine protease NS3 activity to achieve high catalytic efficiency for a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-   diphosphate + H(+) + phosphate.4. The designed protein must have at least one Peptidase C53, RdRp catalytic, Peptidase C74, Helicase ATP-binding, Peptidase S31 domain. The designed protein sequence is ",
    "response#1": "MELNHFELLYKTSKQKPLGVVEPVYNKAGEPLFGEASEIHPQSTLKLPHDRGRAEIRTKLKSLPRKGDCRSGNNQGPVSGVYIKPGPVFYQDYAGPVYHRAPLEMFAETQFCEVTKRLGRVTGSDGKLYHIYVCIDGCIMLKLVSKSESKVLKWVRNITDCPLWVASKFKIVKEIAETQHGTIVIRVQYEGDGSPCKIPFEITDLEKRHVLGRLITVNPIVTEKDAPVNIEAEPPFGDSYIIVGVEPGQLKLNWFKGPAVCKKITEHEKCHINILDKLTAFFGIMPRGTTPRAPVRFPTSLLKVRRGLETGWAYTHQGGISSVDHVTAGKDLLVCDSMGRTRVVCQSNNRLTDETEYGVKTDSGCPDGARCYVLNPEAVNISGSKGAVVHLQKTGGEFTCVTASGTPAFFDLKNLKGWSGLPIFEASSGRVVGRVKVGKNEESKPTKIMSGIQTVSKNTADLTEMVVSGGTIARATHGFTGLFAPGAQQNIRLIHTDGSWHINSTALNCNDSLNTGFLAGLFYYHXFNSSGCPARLASCRRLDDFSQGWGPLTYNSSSTAGINRPYCWHYAPRQCEVVPAKNVCGPVYCFTPSPVVVGTTDSRGVPTYNWGENETDVIILNSTRPPNGAWFGCTWMNGTGFTKACGAPPCAVQSVNGTWPCPSDCFRKHPETTYAKCGSGPWLTPRCLVDYPYRLWHYPCTVNYTIFKVRMYVGGIEHRLNAACNWTRGEPCDLEHRDRAELSPLLLSTTQWQVLPCSFTTMPALSTGLIHLHQNIVDVQYLYGVGSAVVSWALKWEYIVLAFLLLAD",
    "response#2": "HQPIPELRCDIQCNATWHSDLWYLAQEVAGELWYVEYLWKLPYDFWRGVLALNPVMVFVAILLGLEQRFVMLVLVIMVGGMARAAPVGDLGVRPFEPGPPWDRCDCGQVGRFVWNRGNDTMHCRCPNGDWYWIPSNCQRTAWYEGERLVTWSHGQNHWPLACPQRVQGPVSVVCQWGSRSWYKLVDLDGPLDIHAMVPYGSALCQVRTHIVCAHGALCLGQIWGRTCATCIIDRRPKSCGLCVRDCWPETGRRDTPFHKCGTGSRMTEDLEAVVYVNRSTGLTFHGPVGDLGGGNPTRHPLEAGTYAATLISGTVHLLKCPTSTAPPGLLGFFPGEPPLNVCVTGVETWSEYLGNLALTGGFFEPVFRRCSELAPEPYRVCKGYAWISGAGPEGFITVRGDQQAVSPSWWIPPPRWLHTVGAVAGRQVGGIAGLFMPGAKQNIQLINSNGSWHINRTALNCNDSLNTGFFASLFYINKFNSTGCPERMSSCKXLXHFDQGWGPITYANISGASDDRPYCWHYPPRPCGVEQASKVCGPVYCFTPSPVVVGTTDRKGVPTYNWGGNESDVFLLESLRPPAGGWFGCTWMNSTGFTKTCGAPPCKIRPAGDHNSNTTLTCPTDCFXKHPGATYAKCGSGPWLTPRCLIHYAYRLWHYPCTVNFTIHKVRMFVGGLEHRFEAACNWTRGERCDLEDRDRVEMSPLLFSTTELAVLPCSFSTMPALSTGLIHLHQNIVDVQYLYGISPSITSWVIKWEYVVLAFLLLAD",
    "response#3": "IAGQTARAASGLSSFFNLGPSQRLQLVNTNGSWHINRTALNCNDSLNTGFISALFYSQGHLNLTGCSQRVAACKDLKHFHQGWGPITYANVSGPSEEKPYCWHYAPRPCKKTPASTVCGPVYCFTPSPVVLGTTDRKGAPTYNWGANETDVFLMSSMRPPMGGWFGCTWMNSTGFVKTCGAPPCNIRPGPKGNKTLLCPTDCFRKHPGATYTKCGSGPWLTPRCLVDYPYRLWHYPCTVNYTIHKVRMFVGGVEHRFQAACNWTRGERCDLEDRDRIEMSPLLFSTTELAVLPCSFTPTPALSTGLIHLHQNVVDVQYLYGLSTSIVNWAIKWEYVVLMFLVLADNVGSDVSRSTFGLTALFSPGAQQQLQLVNSNGSWHINRTALNCFDSIQTGFLAALFALRSRVNTTGCVERLSPCHPITHFDQGYGPVTYNYTAPNTDEEPDRRPYCWHYHPPRCGIVPARNVCGPVFCFTPSPVVVGVTDVTGMPTYTSGANETDFLLLNNTRPPLGNWFGCTWMDSEGYTRTCGAPPCNIGGSGNKPLRCTTDCFRKHPEATYARCGTGPWVTPRCLVDYPYRLWHYPCTVNYTVTKVRTYVGGIEHRFDVACNWTRGDPCALEDRDRSVLSPLLLTTTQWQVLPCTFTPMPALSTGLIHLHQNIVDVQYIYGLGSTVVSWAIKWEYVVLLFLLLADHTTGGAAGRTTHGIASLFNPGSKQSLQLINTNGKWHINRTALNCHDNHNTGFVASLFYPDRFNSSGCPERLASCRPLDSWPQGFGPLTSDERDEDLGQPYCWHYAPRSCGVVPAQSVCGPVYCFTPSPVVVGTTDKTGLPTYTWGENETDVFLLNSTRPPAGGWFGCTWMNSTGFTTACGSPPCNIGGLGNDTLQCPTDCFRKHPGATYAKCGSGPWLTPRCLVHYEYRLWHYPCTVNYTIFKVRMYVGGIEHRLDVACNWTRGEPCALEHRDRSELSPLLLSTTQWQVLPCSFTTLPAISTGLIHLHQNIVDVQYLYGVGSVLVSWAVKWEYVVLLFLLLAD"
  },
  {
    "reference": "MLEGKRVLLGVTGGIAAYKAVEIASRLRKLGAEVNVVMTASATEFVTPLTFRSISSRPVYTEMFEEPKVWDVEHISLAKWADVALVAPATANIMAKMAIGLADDFLSTVLLAVTSPVFIAPAMNHAMYHHPATQKNRAILEQRGVRFIGPATGYQACGTEGDGRMSEPEEIVRELEAYFRSKEIMKGQKVLVTAGGTRELLDPVRYIGNFSSGKMGYAIAQAFEEAGADVTLISASTAQVEPANVKLIRVQSALEMYEQVLALYEEQDIVVKAAAVADYRPADFKEQKIKKKGENLVIELVPNPDILAALGERKKHQFLVGFAAETQDVVANALEKLRRKNADMLVVNDVTKPGAGFGSDTNIVSFLFQDGRRIDYPLMSKTEVARELVREIAAIRQGTGIKE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Ideal protein should have a binding affinity for key enzymes in the coenzyme A biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MPRITLGITGGISVYKICELVRLSKKNHFSVQVVMTKDATKFVTPETFRVLSQKEVLIDTFEYTSPERVEHVTIPQETDLFVIAPATANTIAKMANGIADNMLTCIWLAYRGKTLVVPAMNHNMFLHPATQRNLEILKSWGVHIMEPGVGPLACGTEGKGRLPEPPAIYEEILG",
    "response#2": "DGVRIVMTSGGTHEPIDPVRYIGNRSSGKMGRALAIEALARGASVTVITGPCDVPLPEGALVIPVESAAQMAEAVVNLAQDADVFIGAAAVADYRVENPSDSKRKKTGRKFELSLVENPDVLGLVGAVKRAKQILVGFAAETDDLLANAAAKLKKKQLDLIVANEVNTDVSGFGKDTSKAALE",
    "response#3": "MHVVVGVSGGIAAYKACSLVRRFTEGGHDVTVVPTAAALRFVGAPTWAALSGKPVSTEVWTDVHEVPHVRIGQSADLVVVAPATADIMAKAAAGIADDLLTNTLLTARCPVVFAPAMHTEMWEHPATQGNVETLRRRGAHVIEPAEGRLTGADSGKGRFPEPEDVYAAAVAR"
  },
  {
    "reference": "MAKTRTSSLRNRRQLKPAVAATADDDKDGVFMVLLSCFKIFVCFAIVLITAVAWGLIMVLLLPWPYMRIRLGNLYGHIIGGLVIWIYGIPIKIQGSEHTKKRAIYISNHASPIDAFFVMWLAPIGTVGVAKKEVIWYPLLGQLYTLAHHIRIDRSNPAAAIQSMKEAVRVITEKNLSLIMFPEGTRSRDGRLLPFKKGFVHLALQSHLPIVPMILTGTHLAWRKGTFRVRPVPITVKYLPPINTDDWTVDKIDDYVKMIHDVYVRNLPASQKPLGSTNRSN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. We require a protein that can specifically bind to the metabolites produced in the CDP- diacylglycerol from sn-glycerol 3-phosphate: step 2/3, Phospholipid metabolism pathway.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The protein's functional design necessitates the inclusion of a HXXXXD motif motif. The designed protein sequence is ",
    "response#1": "TTVYGYEKTQVEGPAVVICNHQSSLDILSMASIWPKNCVVMMKRILAYVPFFNLGAYFSNTIFIDRYNRERAMASVDYCASEMKNRNLKLWVFPEGTRNREGGFIPFKKGAFNIAVRAQIPIIPVVFH",
    "response#2": "GICVANHTSPIDIVILANDGCYAMVGQVHGGLMGVIQRSMVRSCPHVWFERSEMKDRHAVAKRLKDHISDKTKLPILIFPEGTCINNTSVMMFKKGSFEFGGTIYPVAIKYR",
    "response#3": "GICVANHTSPIDIVILANDGCYAMVGQVHGGLMGVIQRSMVRSCPHVWFERSEMKDRHAVAKRLKDHISDKTKLPILIFPEGTCINNTSVMMFKKGSFEFGGTIYPVAIKYR"
  },
  {
    "reference": "MLTAIRKNGLTLAIFACATTGLVALTQYLTEDQIKLQEQKQLLSVLNQVIPETMHDNALTQSCTLVTSPELGTMHAMPTYIATKNGEPTAIAIESIAPDGYNGEIKVITGIDNQGKILGTRVLSHQETPGLGDKIDLRVTSWILGFTGKQVTEDNWNSWKVRKDGGDFDQFTGATITPRAVIKAVRNTVNYVNQSRDEILSQPLNCAGDNQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The protein requires FMN to activate a critical catalytic site.3. The protein can be targeted to plasma membrane and perform FMN binding, electron transfer activity.4. The protein must exhibit the following characteristics: Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.. The designed protein sequence is ",
    "response#1": "YSLAIRRWHWGNALLFVLLLLSGLFGHFSLGPVALMVQVHTWCGFALLAFWVGFVLINLTTGNGRHYRVNFSGLVTRCIRQTRFYLFGIMKGEAHPFVATEQNKFNPLQQLAYLAIMYALVPLLIITGLLCLYPQVAGLGPVMLVLHMALAIIGLLFICAHLYLCTLGDTPGQIFRSMVDN",
    "response#2": "YSLAIRRWHWGNALLFVLLLLSGLFGHFSLGPVALMVQVHTWCGFALLAFWVGFVLINLTTGNGRHYRVNFSGLVTRCIRQTRFYLFGIMKGEAHPFVATEQNKFNPLQQLAYLAIMYALVPLLIITGLLCLYPQVAGLGPVMLVLHMALAIIGLLFICAHLYLCTLGDTPGQIFRSMVDN",
    "response#3": "SANLLILFLGLEALTFAFYILVAIDLNRAESAEAGLKYLLLGAISAACIAFGIALLYAAAGTLAIPEVVRLTLSAGAQDPIALAGWGLLLIGIAFKISLVPAHHWTPDVYQGAPTPVVAFLSTASKGAAIAFLLLLLPSGSGFKTLHTPLWWLSLLSMLVGNLAALLQTNLKRMLAYSSIAQMGYLVLALLTGSSEGFAAVILYVVVYTAMNLAAFGAVASLTESVGMENVEDYRGVGYSRPFQAGILALALFALAGIPPTAGFIGKFFIFYAAFRGGEIPLAIVGILALA"
  },
  {
    "reference": "MWLSLHHLGGPHPATPPPAGPNVGQSVFRLYSPGGRMEDWCTLDPFQAGGPSCPGFNSSHGLILISPGWSLDGMMESWVLRLASGLEQSLAEVNVVVTDWLRLAHQHYPIAVQNTRAVGKDVATLLRWLQDHYKLSPKRVHLIGYSLGAHISGFAGSYFRGSEKLGRITGLDPAGPLFEGMSPVDRLSPDDADFVDAIHTFTQERMGLSVGIKQPVAHQDFYPNGGTFQPGCDLHNIYHHITEYGLHGFEQTVKCAHERSVHLLIDSVLHRDRQSVAYVCRDQDAFERGLCLDCRRRRCPRLGYHAHHGHGGTVGKKLFLKTRARMPYKLYHYQFKVQFTNHLKGMRPSLIIALTGTNEEIGELPIQFEEEVSGNRTYAFLITLERDIGDLMMLRVQWQSTALWKSVWTRMHTILPWGNMAAPPQLSVGRISVKVGETQKRTSFCPATEDGQHFQASEEKLFVRCQENHQKQRRKKHNLA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be optimized for high-density lipoprotein particle-specific folding and stability, while maintaining triglyceride lipase activity. The designed protein sequence is ",
    "response#1": "FGRRSRAIETKKTLQEMKTKFLLFEDKASKGCQIQLNLPDTLQECGFNSSLPLVMIIHGWTVDGKLENWIWQMVAALKSQPAQPVNVGLADWIPLAHQHYTIAVRNTRLVGQEVAALLQWLEESVSLSPSNVHLIGYSLGAHVSGFAGSYMGRKHKIGRITGLDAAGPLFEGASPSDRLSPDDANFVDAIHTFTREHMGLSVGIKQPIAHYDFYPNGGSFQPGCHFLELYKHIAEHGLQAITQTIKCAHERSVHLFMDSLLHTSMQSTAYLCSDMDSFLQGRCLSCKKGRCNTLGYHVRQQPHGKSKKLFLLTRAQSPS",
    "response#2": "FGRRSRAIETKKTLQEMKTKFLLFEDKASKGCQIQLNLPDTLQECGFNSSLPLVMIIHGWTVDGKLENWIWQMVAALKSQPAQPVNVGLADWIPLAHQHYTIAVRNTRLVGQEVAALLQWLEESVSLSPSNVHLIGYSLGAHVSGFAGSYMGRKHKIGRITGLDAAGPLFEGASPSDRLSPDDANFVDAIHTFTREHMGLSVGIKQPIAHYDFYPNGGSFQPGCHFLELYKHIAEHGLQAITQTIKCAHERSVHLFMDSLLHTSMQSTAYLCSDMDSFLQGRCLSCKKGRCNTLGYHVRQQPHGKSKKLFLLTRAQSPR",
    "response#3": "TRFLLFPAERSPGCEIGWHRPGSLQECGFNSSRPLVLIIHGWSVDGVLESWVHQLVAALRTQPVNVGLADWLPLAHNHYAISVRSARLVGQEVAAVLRWLEESEDFSRSNVHLVGYSLGAHVSGFAGSSIGGKHKIGRITGLDAASPLFEGTSPSERLSPEDAVFVDAIHTFTREHMGLSVGIKQPIAHYDFYPNGGTFQPGCHFLDLYKHFAQHGLNAIPQTIKCAHERSVHLFIDSLLHEDMRSTGYQCSNMGSFSQGLCLNCKKGRCNSLGYHARQELRGKSKSLYLVTRR"
  },
  {
    "reference": "MLKTLSKVSKNLSNTSAFLVKSKPLYISFCTCKAYFEDNTSLSRRFFSAKKSLQFKSLIRKYSEDSTLAWRDYENYNDDDLLYQRELSEIYNKCLKALHPFYPVFEVNSNLYYQLLMFTNYSNPKDNGFSKLLFSQYSDEKKVLVNFVYNVLRLDPDLQRQKRILESIISAALNICVSTVNLSRTDLKSSLKIILQTIDLFKYDSSFSSIYFRKDGPYHKVEKLLKSANIRSSLNSKTFNDFRTLFLLLIRDYSLFHGNSSHCMLANSMLPPSIAFSKMEKAEIFLRLRAFFQLMEHIDNERITPSEQFLDKVFISLLSAKNSCSLKMWLIHSFNKGWPVKIEFFVSFFKNFSNIVEGSMEVYSAYLHIIRDHYNLSDLADVQAIFLSRFILLRKKEDCKKLLEHVLPIRQLFLLNCYSLLNVLANYVVVFKDRLIFQEINQNWEEHKGQIPENFIFSLLKMFGEMQENQGFLNACIYYINKKKNLNEVSRYKINLLLLQGVNSFEVKDFYRKRGIEIMPKNLPRFFCYCLKKNKLSYALKYIRLSGLQFDYIVDCFRNSSDWTRTLISILSKKMGTEFAIRFLKIISFPLLSNDKVLREIEFFAIHEVNFRSLKWVADQQLRILPGWSSRPQKATFPLCTVHLKK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be optimized for mitochondrion-specific folding and stability, while maintaining translation activator activity. The designed protein sequence is ",
    "response#1": "LHHVMGGLEGMQGAWGYVQGGMGALSDAIASSATTHGASIFTEKTVAKVQVNSEGCVQGVVLEDGTEVRSKVVLSNTSPQITFLKLTPQEWLPEEFLERISQLDTRSPVTKINVAVDRLPSV",
    "response#2": "LHHVMGGLEGMQGAWGYVQGGMGALSDAIASSATTHGASIFTEKTVAKVQVNSEGCVQGVVLEDGTEVRSKVVLSNTSPQITFLKLTPQEWLPEEFLERISQLDTRSPVTKINVAVDRLPSX",
    "response#3": "NKTHLDALLEVIRVSQPNFLLNDETRMNFIFILTILANAHEWERVYKLWDAALENSVPINPGSTFKPDTRPWAYLIDLVAKIGDPL"
  },
  {
    "reference": "MSMSPKHTTPFSVSDILSPLEESYKKVGMEGSNLGAPLAAYRQSQVAQPAMQQHPMGHNGTVTAAYHMTAAGVPQLSHTAMGGYCNGNLSNMSELPPYQETMRNSASATGWYGANADPRFPSISRFMGPTSGMNMSGMGGLGPLGDVSKNIAPLQSAPRRKRRVLFSQAQVYELERRFKQQKYLSAPEREHLASMIHLTPTQVKIWFQNHRYKMKRQAKDKAAQQQMQQDSGSCQQQQQSPRRVAVPVLVKDGKPCQAGSNAPSAAGLQGHQQQGSAATAITVSSNGTSLSQHSNHQSSSAGQSPDLAQHSASPSSLQSQVSSLSHLNSSSSDYGAAMSCSTLLYGRTW",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be involved in hypothalamus development, positive regulation of circadian rhythm, negative regulation of cell migration, negative regulation of epithelial to mesenchymal transition, forebrain neuron fate commitment by localizing to nucleus, transcription regulator complex and exhibiting RNA polymerase II cis-regulatory region sequence-specific DNA binding, RNA polymerase II-specific DNA-binding transcription factor binding, DNA-binding transcription factor activity, RNA polymerase II-specific, intronic transcription regulatory region sequence-specific DNA binding, enzyme binding.2. The designed protein should contain one or more Homeobox domains that are essential for its function.3. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "PDFSSLPSFLPQTPSSRPMTYSYSSNLPQVQPVREVTFREYAIEPATKWHPRGNLAHCYSAEELVHRDCLQAPSAAGVPGDVLAKSSANVYHHPTPAVSSNFYSTVGRNGVLPQAFDQFFETAYGTPV",
    "response#2": "DSDTDPRELEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAR",
    "response#3": "PDFSSLPSFLPQTPSSRPMTYSYSSNLPQVQPVREVTFREYAIEPATKWHPRGNLAHCYSAEELVHRDCLQAPSAAGVPGDVLAKSSANVYHHPTPAVSSNFYSTVGRNGVLPQAFDQFFETAYGTPS"
  },
  {
    "reference": "MEKAGTGTGTQSDLSAGWERVSEELSDSQSNGSMHSTGDAPFWRDQEDIVISGISGRFPRSENVQEFGDLLMAGEDLITEDDLRWPPGIFDLPRRHGKLKDLKKFDAQYFGVTPKQANFLDPQVRKLLEVTLEAIVDAGLNPVELRGSRTGVFVGCSASETGGALTQDPETVTGYTLTGCVRSMFSNRLSFTFDLRGPSFSVDTACSSSLCALQLAVDAIRQEQCDAAVVAGAHLTLTPTAALQFLRLGMLSDKGSCRSFDASGDGYCRTEGVAAILLQRQSVARRIYATVVHAKSNTDGYKEQGITFPSGERQAALLEEVYREAGIDPNTVTYVETHGTGTKVGDPQEANAICQVFCQNRSSPLLIGSVKSNMGHAEPASGLCSIAKVLIAMERGFIPPNLHYNEPNPYIPGLVDGRLQVVTEKTPFPRGIVGVNSFGFGGSNTHVILRAANHAERKAAPASFMKIVAYSGRSAEAVMHVFDCVAAEPENVYLQQLLANQANMPPKDTPYRGYMIMNRDKIPLPANAKRDPDQAPLKDVQKIMITEPKQIYFVYSGMGSQWAGMARQLMSIPAFDESLRISSAAVKEYGLDVYEMLQSEDPELYKNNTLNCMLAITAIQIALTDVLFLMGLTPDGIIGHSTGEMGCGYADGGLTREQTMRLAYHRGATIMNSDEKIEGGMAAVGLTWEEASKRCPEGVVPACHNSVDSVTVSGNAEQVERFVEELKKEDIFAKAVDSSGIPFHSPIMRKVKDKMLSAMRTVVPDPKP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain..2. The designed protein must be optimized for palmitoyl-[acyl-carrier-protein] hydrolase activity, enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity.3. A protein that is capable of catalyzing reactions within the fatty acid biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MEEIVVAGISGRLPESNNLEEFWENLFNGVDMVTEDDRRWKPGLYGLPKRNGKLKDISRFDAAFFGVHPKQAHTMDPQLRLMLEIAYEAIVDGGLNPTELRGSRTGVYIGVSGSEAGEAFSRDPEELLGYSMTGCQRAMFANRLSYFFDFNGPSTAIDTACSSSLLALENAFNAIRHGHCDAALVGGVNLLLKPNTSVQFMKLGMLSPEGTCKSFDSSGNGYCRSEAAVAVLLTRRSMAKRVYATVLNAGNNTDGYKEQGVTFPSGEMQQRLVRSLYQEVNITPDQVEYIEAHGTGTKVGDPQEVNGIVSVFCHSKRDPLLIGSTKSNMGHPEPASGLAALAKVVLSLERGVWAPNIHYNGPNTDIPALSDGRLRVVNEPIPVRGGIVGINSFGFGGSNVHVILRPAGKAAPPRPPRPNPDIPALHDGSLKVVCKPTPVKGGANAHVILRPNKNKCQPLETCNVPRLVQISGRTQEAVETLIQESRKHGGCSPFVSLLSDISTVPVSSMPYRGYTPNPDIPALHDGSLKVVCKPTPVKGGANAHVILRPNKNKCQPLETCNVPRLVQISGRTQEAVETLIQESRKHGGCSPFVSLLSDISTVPVSSMPYRGYTPNPDIPALHDGSLKVVCKPTPVKGGANAHVILRPNKNKCQPLETCNVPRLVQISGRTQEAVETLIQESRKHGGCSPFVSLLSDISTVPVSSMPYRGYTPNPDIPALHDGSLKVVCKPTPVKGGANAHVILRPNKNKCQPLETCNVPRLVQISGRTQEAVETLIQESRKHGGCSPFVSLLSDISTVPVSSMPYRGYT",
    "response#2": "IVVAGISGRLPESDNLDEFWQNLFNGVDMVTEDDRRWKPGLYGLPRRNGKLKEIDRFDAAFFGVHPKQAHTMDPQLRLMLEISYEAIVDGGINPTSMRGTNTGVYIGVSGSEAGEAFSKDPEELLGYSMTGCQRAMFANRLSYFFDFNGPSTAIDTACSSSLLALENAFNAIRHGQCDAALVGGVNLLLKPNTSVQFMKLGMLSPDGACKSFDVSGNGYCRSEAAVAVLLTKKSMAKRVYATVLNAGNNTDGYKEQGVTFPSGDMQQRLVRSLYQEANISPEQVEYVEAHGTGTKVGDPQEVNGIVGVFCQSKREPLLIGSTKSNMGHPEPASGLAALAKVLLSLEHGMWAPNIHFNNPNPDIPALLDGRVRVVSEPIPVRGGIVGINSFGFGGSNVHIILRPPGYDWLPNLQSAMRNETKTLVVVENQPLSGVLGFVNCLRREPGGTNVKCVFVQNGNIKFNLSDPLFREQLDKDLAINVLNQDVWGSYRHLPILWFVFSGMGSQWPGMGIELLKFPVIAKAVQKCDDVLQPRGIDIYDILTNKDKSTFNNILNSFVGIAAMQIGLVDLLTSVGIVPDNIIGHSVGELGCAYADGCLTAEQMILSAYSRGLASIETKVIYGSMAAVGMGYEDIKHMCPPDIEVACHNASDSSTISGPAESMKEFVAQLQAKKIFAKEVLCSNIPYHSRYIAPAGPKLLAYMNEVIPQPKPRSRKWVSTSVPRSKWSTASAKLSSAEYHTNNLLSPVLFERWAERTGGMAFHSYFMDAIAPTLLQQLKKVIRDPQPRSSRWLSTSIPKTQWQESLARTFSAEYNVNNLVSPVLFQEALWQVPENAVVLEIAPHALLQAVLKRGLKTSCTIIPLMKKDHKDNLEFFLSSMGRLYLTGID",
    "response#3": "MEDVVIAGIAGRLPESENLQEFWENLLNGVDMVTEDDRRWKPGIYGLPKRNGTNTMDPQLLCSSFLLIVIYLPIPIGINPGTLRGTDTGVWVGASGSEAGEALSQDPEELLGYSMTGCQRAMLANRISYFFDFKGPSLTVDTACSSSLIALENAYKAICHGRCSAALVGGVNLLLKPNTSVQFMKLGMLSPDGACKSFDVSGNGYCRSEAAVVVLLTKRSMAKRIYATIVNAGVNTDGFKEQGVTFPSGKMQEQLVGSLYRESGIKPEEVEYIEAHGTGTKVGDPQEVNGITGIFCNCKREPLLIGSTKSNMGHPEPASGLAALAKVILSLEHGLWAPNLHFNTPNPDIPALHDGSLKVVCKPTPVKGGANAHVILRPNPDIPALHDGSLKVVCKPTPVKGGANAHVILRPNKNKCQPLETCNVPRLVQISGRTQEAVETLIQESRKHGGCSPFVSLLSDISTVPVSSMPYRGYTYDWLPNLQSAMRNETKTLVVVENQPLSGVLGFVNCLRREPGGTNVKCVFVQNGNIKFNLSDPLFREQLDKDLAINVLNQDVWGSYRHLPSNRWAERTGGMAFHSYFMDAIAPTLLQQLKKVIRDPQPRSSRWLSTSIPKTQWQESLARTFSAEYNVNNLVSPVLFQEALWQVPENAVVLEIAPHALLQAVLKRGLKTSCTIIPLMKKDHKDNLEFFLSSMGRLYLTGIDS"
  },
  {
    "reference": "MTSACEAVPQLGLVCLTVGPEVRFRTVTLSRYRALSPAEREAKLLDLYSSNIKTLRGAADYCAAHDIRLYRLSSSLFPMLDLAGDDTGAAVLTHLAPQLLEAGHAFTDAGVRLLMHPEQFIVLNSDRPEVRESSVRAMSAHARVMDGLGLARTPWNLLLLHGGKGGRGAELAALIPDLPDPVRLRLGLENDERAYSPAELLPICEATGTPLVFDAHHHVVHDKLPDQEDPSVREWVLRARATWQPPEWQVVHLSNGIEGPQDRRHSHLIADFPSAYADVPWIEVEAKGKEEAIAALRLMAPFKKD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. Mn(2+) is required for the proper folding and stability of the protein.2. The protein must exhibit the following characteristics: Recognizes pyrimidine dimers and cleave a phosphodiester bond immediately 5' to the lesion.; Removal of UV LIGHT damaged nucleotides. The designed protein sequence is ",
    "response#1": "PELPEVETVRRGLEQKLKNFIIKRVEICRESTVAYPIDKIDFIEGLQNSLLYKWNRRGKYLIAELKKTVSNNNDANEISFVENGVLVVHLRMTGYFTFNNTLTNPCKHTRIRLFDNNNNELRYIDVRSFGQML",
    "response#2": "PEMPEVETVRRTLTPLVKGKTIAKINIWYPKIIVNDPAEFVKKLTNKRILKIDRYGKYLLFRFNDDLTMVSHLRMEGKYHLVSPDTPKGKHEHVEFIFTDGTALRYDDVRKFGRMR",
    "response#3": "PEGPEIRRAADKLEAAIKGEPLTNVWFAFPQLQPYQTQLTGQRVTHIATRGKALLTHFSGGLTLYSHNQLYGVWRVVDAGVEPQSNRVLRVRLQTASKAILLYSASDIDILTAEV"
  },
  {
    "reference": "MEQGLRSDGNNPPSIDKTRVLDVKPLRCLAPVFPSPNGMSSVSTPQPSPFVCVPPTGPFPPGVAPFYPFVAPNDSGRPGESSQQTPSGVPNQGGPFGFAQPISPVPLNSFRTPTTANGNSGRSRRAVDDDDYSNSQDQNDQFASGFSVHVNNVEDSGTGKKRGRPKKPRRAQQAEGLTPVEVDVEPLLTQLLTSFKLVDLDQVKKADGDKELAGRVLLVFDLFRRRMTQIDESRDGPGSGRRPDLKASNMLMTKGVRTNQTKRIGNAPGIEVGDIFFFRMELCLVGLHAPTMAGIDYMSVKLTMDEEPLAVSIVSSGGYDDDGGDGDVLIYTGQGGVQRKDGQVFDQKLERGNLALEKSVHRANEVRVIRGVKDVAYPTGKIYIYDGLYKIQESWAEKNKVGCNVFKYKLLRVPGQPEAFKVWKSIQQWKDGVASRVGVILPDLTSGAESQPVCLVNDVDDEKGPAYFTYIPSLKYSKPFVMPRPSPSCHCVGGCQPGDSNCACIQSNGGFLPYSSLGVLLSYKTLIHECGSACSCPPNCRNRMSQGGPKARLEVFKTKNRGWGLRSWDPIRGGGFICEYAGEVIDAGNYSDDNYIFDATRIYAPLEAERDYNDESRKVPFPLVISAKNGGNISRFMNHSCSPNVYWQLVVRQSNNEATYHIAFFAIRHIPPMQELTFDYGMDKADHRRKKCLCGSLNCRGYFY",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be able to bind S-adenosyl-L-methionine, Zn(2+) ligand in a variety of conditions2. The protein can be targeted to nucleus and perform histone methyltransferase activity, zinc ion binding, histone H3K9 dimethyltransferase activity. The designed protein sequence is ",
    "response#1": "MQSTSMEESGGDDIKVTNDVDADGGPPDFEFVYSDTMLYPDGIPPPELGLGCDCDGPCDPDSETCTCVKRQELYFYDLGLKGFAYDENGKIRENSASIWECNELCG",
    "response#2": "CSTSCIEDCLASEMSCNCAIGVDNGFAYTLDGLLKEEFLEARISEARDQRKQVLRFCEECPLERAKKVEILEPCKGHLKRGAIKECWFKCGCTKRCGNRVVQRC",
    "response#3": "MKDITAGEENVEIPWVNEINEKVPSRFRYMPHSFVFQDAPVIFSLSSFSDEQSCSTSCIEDCLASEMSCNCAIGVDNGFAYTLDGLLKEEFLEARISEARDQRKQVLRFCEECPLERAKKVEILEPCKGHLKRGAIKECWFKCS"
  },
  {
    "reference": "MLNCKMIMKTDILGFNLLALETALIDMGEKPFRGRQIAGWIYKHKSNDFLKMTNICAPLRDKLSKKFLIGKIQPLKTVNDKSSTCKTLWELADGESIESVLIPDKDRLTLCISTQVGCPLNCQFCATGRIGFRRNLAAGEIINQFLQTELGESERITNVVFMGMGEPLLNFDNLLTALEVLIDDLCIGFGARKITVSTAGIPPKIRSLADSGLNVGLAFSLNAASDHLRDLLMPINRRFKLQDNLEALQYYHRKINRRITFEYVLIKGINDSLKDAADLAEITRGIPCKINLIRYNPIDGSQFRPPDNKTVYAFRDFLYPRTPAVTLRESKGANIAAACGQLKAIYSKRET",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs..2. A protein that contributes to rRNA base methylation by functioning in cytoplasm with metal ion binding, 4 iron, 4 sulfur cluster binding, rRNA binding.3. The presence of Radical SAM core domains is critical for the interaction of the designed protein with its intended targets.4. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "AGDRLTLCISSQAGCALRCRFCATGWSGFGRQLSTAEIVGQYRASVRWAREHGRGPVTNVVFMGMGEPLANRRAVFPALSILNGGYGLGARRITVSTVGVIPGIEALARRPEQFRLALSLHAPVSELRAELIPLEKRYPLEQLMAALEGFNRKGGRRITFEYTMIRGVNDDLQLLPRLAELAVRVQAFVNLIPFNPIPYVAWKPSFAGRIRAFREGLERAGVPAAVREPRGRS",
    "response#2": "RPNLLGLTPPALAEALDGHFQARGQPRYRGGQVREWLSAGDARGFGDMTNLPLRERDALGERFRLDEPGVDTVSESADGTVKHLWALRGGEVVESVLIPAGDRLTLCISSQAGCALRCRFCATGWSGFGRQLSTAEIVGQYRASVRWAREHGRGPVTNVVFMGMGEPLANRRAVFPALSILNGGYGLGARRITVSTVGVIPGIEALARRPEQFRLALSLHAPVSELRAELIPLEKRYPLEQLMAALEGFNRKGGRRITFEYTMIRGVNDDLQLLPRLAELAVRVQAFVNLIPFNPIPYVAWKPSFAGRIRAFREGLERAGVPAAVREPRGRDIDAACGQLRASREG",
    "response#3": "SSQAGCGMNCPFCATGQAGLTRNLSTGEIVDQVVAAARLLAEGGVGGSPVRLSNVVFMGMGEPLANYKAVMGALRRLTDKDSHGLGLSRRSITVSTVGLVPRIDQLTEENLPVTLAVSLHAPDDELRDTLVPVNTRWKVSEVLEAADRYSRSTGRRYSIEYALIKDINDQGNLRPIIEVKDATEQDRRIREVHSGIMVIPNKHLKTWLSRLTNDNAQGEYYLTDIFEMAVADGLPVHTAQPSDTWEVAGINSKVQLAELERWYQREIAERLLEQC"
  },
  {
    "reference": "MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYNDTHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSFLYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGYAQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLVLLVATPALITILFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFFSMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKESVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAELFIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINYSINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein's architecture should predominantly feature helix-strand-helix-helix-helix-turn-helix-helix-turn-helix-strand-helix-helix-helix-helix.2. The ideal protein would be one that plays a critical role in dipeptide transmembrane transport through tripeptide transmembrane transporter activity, peptide:proton symporter activity within plasma membrane. The designed protein sequence is ",
    "response#1": "PSPGVAEKWENKDFKVWTFHLRENAKWSDGTPVTAHDFVYSWQRLADPNTASPYASYLQYGHIANIDDIIAGKKPATDLGVKALDDHTFEVTLSEPVPYFYKLLVHPSVSPVPKSAVEKFGDKWTQPANIVTNGAYKLKNWVVNERIVLERNPQYWDNAKTVINQVTYLPISSEVTDVNRYRSGEIDMTYNNMPIELFQKLKKEIPNEVRVDPYLCTYYYEINNQKAPFNDVRVRTALKLALDRDIIVNKVKNQGDLPAYSYTPPYTDGAKLVEPEWFKWSQQKRNEEAKKLLAEAGFTADKPLTFDLLYNTSDLHKKLAIAVASIWKKNLGVNVNLENQEWKTFLDTRHQGTFDVARAGWCADYNEPTSFLNTMLSDSSNC",
    "response#2": "PSPGVAEKWENKDFKVWTFHLRENAKWSDGTPVTAHDFVYSWQRLADPNTASPYASYLQYGHIANIDDIIAGKKPATDLGVKALDDHTFEVTLSEPVPYFYKLLVHPSVSPVPKSAVEKFGDKWTQPANIVTNGAYKLKNWVVNERIVLERNPQYWDNAKTVINQVTYLPISSEVTDVNRYRSGEIDMTYNNMPIELFQKLKKEIPNEVRVDPYLCTYYYEINNQKAPFNDVRVRTALKLALDRDIIVNKVKNQGDLPAYSYTPPYTDGAKLVEPEWFKWSQQKRNEEAKKLLAEAGFTADKPLTFDLLYNTSDLHKKLAIAVASIWKKNLGVNVNLENQEWKTFLDTRHQGTFDVARAGWCADYNEPTSFLNTMLSDSSNX",
    "response#3": "PSPGVAEKWENKDFKVWTFHLRENAKWSDGTPVTAHDFVYSWQRLADPNTASPYASYLQYGHIANIDDIIAGKKPATDLGVKALDDHTFEVTLSEPVPYFYKLLVHPSVSPVPKSAVEKFGDKWTQPANIVTNGAYKLKNWVVNERIVLERNPQYWDNAKTVINQVTYLPISSEVTDVNRYRSGEIDMTYNNMPIELFQKLKKEIPNEVRVDPYLCTYYYEINNQKAPFNDVRVRTALKLALDRDIIVNKVKNQGDLPAYSYTPPYTDGAKLVEPEWFKWSQQKRNEEAKKLLAEAGFTADKPLTFDLLYNTSDLHKKLAIAVASIWKKNLGVNVNLENQEWKTFLDTRHQGTFDVARAGWCADYNEPTSFLNTMLSDSSNC"
  },
  {
    "reference": "MLTIYAVSDSIGETAEQVSKATARQFKERVDVKRVTYIRTFEAVDDFINSIKDPENSMLISTVVLVDIREFLVERCIERGIRIVNVLGPCISTASRVLKTTPTYEPGAVWNMDDRYYKKIEAMEFAMRYDDSKDYNGIKHADVILIGLSRTSKTPLCMYLANKGIKALNVPIMPEIPIPEELFEVDRTKLVGLTIDPIRLIEIRKHRMNKFNQLSSEIQYANGERVLEELEYADKIMRRLRCKIIDVTNRAIEDTALLIMEAIGYNGFRES",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The ADP binding site should be located in a region of the protein that is accessible to the ligand.2. For general function, the protein need meet that Bifunctional serine/threonine kinase and phosphorylase involved in the regulation of the pyruvate, phosphate dikinase (PPDK) by catalyzing its phosphorylation/dephosphorylation.3. A protein with protein serine/threonine kinase activity, phosphotransferase activity, phosphate group as acceptor, ATP binding, ADP binding functions is required to enable protein dephosphorylation, protein phosphorylation. The designed protein sequence is ",
    "response#1": "MDRQTE",
    "response#2": "MENTTKY",
    "response#3": "MICFL"
  },
  {
    "reference": "MGLDLLSTYAPGIFDSLLTWKGVAGLVVAITLGYLIISRLPGQKSRPKLLDLKTGGISFEKVAAVYDDYDKSYGEGDHGELHVKDKNKVFQLANTFYDFVTDGYEWAWGSSFHFSQRMPGLSHAASQMLHESRMASFLRLKPGMKCLDVGCGVGNPGRTVASCSGAEVTGITINEYQIKRAEYHNKRTGLVGYFKPVVGNFCAMPFKDKTFDAAFAMDSTCHAPKLEDVYSEVFRVLKPGGLFATYEWVSTKDYDPNNSRHVKVMNSIIFGNGLPNIRSWKQAEDAGKNVGFKLVTSFDLATAPPVGKPWYYVPELMVKYGLLTIQKALVRGACNVGLLPNEGWKVCNMVADMVPNLVEGGATNIFTPMHLLIFEKPK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. The protein should be designed to localize specifically to endoplasmic reticulum, membrane in order to perform its role in methylation more effectively.3. For general function, the protein need meet that Unable to methylate cycloartenol, zymosterol or lanosterol; Produces mainly dimethylated squalene.; Methylates both C-3 and C22 positions, but only C-3 position in monomethylated products; Converts squalene to mono- and dimethyl derivatives, but not to tri- and tetramethylated products The designed protein sequence is ",
    "response#1": "LTVFRMNKWGRLVMHNLIGVLEACSIAPKGTRKTADGLAKGADALVEGGKRKLFTPMYLMVGKKPL",
    "response#2": "VWETSFTLQALVESGLYEKEAFKPDIAKALEFLDRQQIRTQYEGSGYRYNSLGAWPFSNITQGYTVSDTTSEALRAVLLVQSLPDFEKLVDIPRLRLSVDVILGMQNENLGFASYEPARTGEWMELLNPAEVFGNIMVEYSYPECTTSVILALRAFTKYDPGYRRDEIENTIENALEYVVKMQRPDGSWYGSWAICFTYAAMFATGSLASAGRYYENCPVQKKACEFLLSKQRPDGGWSESYMACVTGVYTETESSLVTQTGWALDALINAKYPDRKPIEKGIKFLMASQKSDGSWQQKSMEGIFNKNVAIAYPNYKLYFSIYTLGKFR",
    "response#3": "VWETSFTLQALVESGLYEKEAFKPDIAKALEFLDRQQIRTQYEGSGYRYNSLGAWPFSNITQGYTVSDTTSEALRAVLLVQSLPDFEKLVDIPRLRLSVDVILGMQNENLGFASYEPARTGEWMELLNPAEVFGNIMVEYSYPECTTSVILALRAFTKYDPGYRRDEIENTIENALEYVVKMQRPDGSWYGSWAICFTYAAMFATGSLASAGRYYENCPVQKKACEFLLSKQRPDGGWSESYMACVTGVYTETESSLVTQTGWALDALINAKYPDRKPIEKGIKFLMASQKSDGSWQQKSMEGIFNKNVAIAYPNYKLYFSIYTLGKFL"
  },
  {
    "reference": "MLKRSMNIADYDPVLWQAIQDENLRQEEHIELIASENYASPRVMEAQGCQFTNKYAEGYPGKRYYGGCEYADIVEQLAIDRAKALFGADYANVQPHSGSQANAAVYMALLNPGDTILGMSLAHGGHLTHGASVSFSGKIYKAEQYGITSEGLIDYDALRKQAHEVKPKMIVGGFSAYSQIVDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPSPMPHAHVVTTTTHKTLAGPRGGLILANGNEELYKKLNSAVFPAGQGGPLVHVIAAKAVCFKEALEPEFKTYQAQVVKNAKAMVKVFKQRGYNVVSNGTENHLFLVDLVNHGLTGKAADAALSRANITVNKNAVPNDPQKPFVTSGIRVGTPAVTRRGFNEEDVAELAGWMCDILDSMNKDNHEQVIADTKEKVLAICKRLPVYP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The presence of pyridoxal 5'-phosphate is crucial for the protein to function optimally under specific conditions.2. The (6S)-5,6,7,8-tetrahydrofolate binding site should be stable and able to withstand changes in environmental conditions.3. We need a protein that can perform (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O =   (6S)-5,6,7,8-tetrahydrofolate + L-serine with high efficiency.4. For general function, the protein need meet that Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier; This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules5. A protein that contributes to tetrahydrofolate interconversion by functioning in cytoplasm with pyridoxal phosphate binding, glycine hydroxymethyltransferase activity.6. A protein that can modulate the overall flux of metabolites through the glycine from L- serine: step 1/1, Amino-acid biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MKTDPEIAEAIRLETRRQAGKLELIASENFVSEAVLEAQGCIMTNKYAEGYPGKRYYGGCEYVDIAENLAIERCKALFGADYVNVQPHSGTQANMAVYFSALSVGDTILGMNLAHGGHLSHGSPANFSGKFYNVVPYGVDRETETIDYNQVEDLALQHKPRMIVVGASAYPRTIDFEKFRAIADKVGALVMADIAHIAGLVATGLHPSPVPVCEYVTSTTHKTLRGPRGGLVMCQASYQKTLSSRVFPGVQGGPLMHIIAAKAVAFKEALTDEFKDYQSQIVKNAQALAKELIGRGYRLVSGGTDNHLLLMDLTDKGLTGKEAQESLDSAGITVNKNGIPFDTRGPMVTSGIRIGTPALTSRGMKEEEMRTIARLIADALADPDDAALQAVAADHAVAAAIADEETRQRDSIELIASENFVSRAVLDAQGSVLTNKYAEGYPHRRYYGGCANVDAIEDLVIARVNQLFGSAYANVQPHSGSQANQAVFLALLAPGDTILGLDLKAGGHLTHGAPVNMSGRWFKAVSYGVDPETHRIDMDQVAVQARQHRPRLLIAGGSAYPRIIDFGRFRQIADEVGAILMVDMAHFAGLVAGGVYPSPVPFADVVTSTTHKTLRGPRGGFVLTNDANIAKKINSATFPGLQGGPLMHVIAAKAVAFGEALQPEFGAYAQAVVENCRVLAQALADGGLTITSGGTDCHLAVVDLRPFGVTGNIAEQALESVGITLNKNAIPNDPEKPMVTSGIRVGTAAGTSRGFGADQYREIAGLYDAHKQLRAKLVPFAGYEMPVQYTSVMDEHLAVRNRAGLFDVSHMGLFEFSGENAHLLLQTLTTNDVSPIAISDSQYSFLLAPDGSVIDDVWVYR",
    "response#2": "MKTDPEIAEAIRLETRRQAGKLELIASENFVSEAVLEAQGCIMTNKYAEGYPGKRYYGGCEYVDIAENLAIERCKALFGADYVNVQPHSGTQANMAVYFSALSVGDTILGMNLAHGGHLSHGSPANFSGKFYNVVPYGVDRETETIDYNQVEDLALQHKPRMIVVGASAYPRTIDFEKFRAIADKVGALVMADIAHIAGLVATGLHPSPVPVCEYVTSTTHKTLRGPRGGLVMCQASYQKTLSSRVFPGVQGGPLMHIIAAKAVAFKEALTDEFKDYQSQIVKNAQALAKELIGRGYRLVSGGTDNHLLLMDLTDKGLTGKEAQESLDSAGITVNKNGIPFDTRGPMVTSGIRIGTPALTSRGMKEEEMRTIARLICKALNTPPDQDYVRETKTRYRTFMKKRYADTDV",
    "response#3": "MKTDPEIAEAIRLETRRQAGKLELIASENFVSEAVLEAQGCIMTNKYAEGYPGKRYYGGCEYVDIAENLAIERCKALFGADYVNVQPHSGTQANMAVYFSALSVGDTILGMNLAHGGHLSHGSPANFSGKFYNVVPYGVDRETETIDYNQVEDLALQHKPRMIVVGASAYPRTIDFEKFRAIADKVGALVMADIAHIAGLVATGLHPSPVPVCEYVTSTTHKTLRGPRGGLVMCQASYQKTLSSRVFPGVQGGPLMHIIAAKAVAFKEALTDEFKDYQSQIVKNAQALAKELIGRGYRLVSGGTDNHLLLMDLTDKGLTGKEAQESLDSAGITVNKNGIPFDTRGPMVTSGIRIGTPALTSRGMKEEEMRTIARLIAEALKDEENKDHIKQREDKEIYDLIEKELVRQQKGIELIASENIVSPAVMEAMGSYLTNKYAEGYPNKRYYGGCHVVDEIEQIAIDRAKELFGAEHANVQPHSGSQANMAVYFAVLEPGDTVLGMDLSHGGHLTHGSPVNFSGKLFNFVSYGVDKETEMIDYENVRKLAIENKPKLIVAGASAYARILDFPKFREIADEVGALLMVDMAHIAGLVAAGVHPSPVPYSDFVTTTTHKTLRGPRGGLILCKEKYAQILNKNIFPGIQGGPLEHIIAAKAVCFKEALDPSFKTYGENVVENCKELAEQLIARGFKIVSGGTDNHVFLVDLNNKDITGKEAEALLDSVGITVNKNTVPNETRSPFVTSGIRIGTAAITTRGFVKDDMAEIAAVEVTDPAVYAAISAEAKRQRNNIELIASENFASPAVMEAQGSLLTNKYAEGYPGRRWYGGCENVDVIEQLAIDRLKDLFSAEHANVQAHSGSQANTAVYFSVLKPGDRILTMDLAHGGHLTHGHKANFSGKFYDVVHYGVSQENECIDYDLLEKMAHESKPALLTCGASAYSRTIDFERMSKIARDVGAYLFVDMAHIAGLVAGGVHPSPVPFADFVTSTTHKSLRGPRGGVIMCKEEFAKKIDAQVFPGIQGGPLMHVIAAKAICFGEALKPEFKQYQQQIVKNAQAMASRLAEHGFRIVSGGTDNHVMLVDLRSKNLNGTLASHALDEAGITVNKNSIPFDTGTPMKPSGIRIGTPAVTTRGMKEADIVQVADF"
  },
  {
    "reference": "MKILIATGNRHKVGEIEAILAGSAAADAALKFLSLADFPDLLPVEEDGETLEQNAVKKAVTAALSTGLWTLADDTGLEVEALDGAPGVYSARYAGPDCDFKANCDKLVGEMKGRRNRRAMFRCVVALSSPQGQVRIQVGSLEGRITETPFGGNGFGYDPVFLVPERDKTLAEMNDEEKNEISHRGEALRAVAPWLREIGNGP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+) binding. The designed protein sequence is ",
    "response#1": "VTIALIGKYIDLPDAYLSVAEALRAGGFAHGSKVNIKWVVSDDCETTEGAHAQLADVDAICVPGGFGVRGVDGKIGALRYARENKIPTLGLCLGLQSMVMEYARNVAGLEGASSTEFDPDTAVPVIATMDEQVDILDGGDLGGTMRLGLYEAQFASGSLAEELYGAASASERHRHRWEVNNRYVEQLADAGLRFSGTSPDGKLVEYVELPKEVHPFYISTQAHPELRSRPSEPHPLFSGF",
    "response#2": "VTIALIGKYIDLPDAYLSVAEALRAGGFAHGSKVNIKWVVSDDCETTEGAHAQLADVDAICVPGGFGVRGVDGKIGALRYARENKIPTLGLCLGLQSMVMEYARNVAGLEGASSTEFDPDTAVPVIATMDEQVDILDGGDLGGTMRLGLYEAQFASGSLAEELYGAASASERHRHRWEVNNRYVEQLADAGLRFSGTSPDGKLVEYVELPKEVHPFYISTQAHPELRSRPSEPHPLFSGFAN",
    "response#3": "LPDAYLSVAEALRAGGFAHGSKVNIKWVVSDDCETTEGAHAQLADVDAICVPGGFGVRGVDGKIGALRYARENKIPTLGLCLGLQSMVMEYARNVAGLEGASSTEFDPDTAVPVIATMDEQVDILDGGDLGGTMRLGLYEAQFASGSLAEELYGAASASERHRHRWEVNNRYVEQLADAGLRFSGTSPDGKLVEYVELPKEVHPFYISTQAHPELRSRPSEPHPLFSGF"
  },
  {
    "reference": "MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVVKGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSLLQDIKVSQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Binds one copper per polypeptide..2. The engineered protein is expected to adopt a strand-turn-strand-helix-strand-strand-strand-strand-based conformation.3. The designed protein should be involved in response to silver ion, response to toxic substance, copper ion export by localizing to periplasmic space and exhibiting copper ion binding, transition metal ion binding, metallochaperone activity. The designed protein sequence is ",
    "response#1": "QGLRSRHESGEGEVKYLERFTERTELLRGIENYRVVILEANPNTFVLPYHKDAESVIVVTRGRATLTFVSQERRESFNLEYGDVIRVPAGATEYVINQDSNERLEMVKLLQPVNNPGQFREYYAAGAQSTESYLRVFSNDILVAALNTPRDRLG",
    "response#2": "QGLRSRHESGEGEVKYLERFTERTELLRGIENYRVVILEANPNTFVLPYHKDAESVIVVTRGRATLTFVSQERRESFNLEYGDVIRVPAGATEYVINQDSNERLEMVKLLQPVNNPGQFREYYAAGAQSTESYLRVFSNDILVAALNTPRDRLG",
    "response#3": "LKSQRSSYSNQFGQFFEACPEEHRQLQEMDVLVNYAEIKRGAMMVPHYNSKATVVVYVVEGTGRFEMACPHDVSSQSYEYKGRREQEEEESSTGQFQKVTARLARGDIFVIPAGHPIAITASQNENLRLVGFGINGKNNQRNFLAGQNNIINQLEREAKELSFNMPREL"
  },
  {
    "reference": "MLISQLISELPIQIIRGDATIAITDVYEDSRHVSPGSLFIARSGTATDGSSFITDAIASGATAVLCTKIPENASEQVTWLTSETVDTQLTGQLAERYFDHPSQKLKLIGITGTNGKTTTAYLTRRLLRQSGVMCGMIGTIEIDDGINRNTAQLTTPGSVELSRLLAKMVANGCTAAVMEVSSHALDQGRASAMTFHAAVFTNLTGDHLDYHGSMANYATAKAKLFEGLGPDNWAILNTDDPYSQEMIANSKARQLWCRVCDVAPVESPQETEEQPTFCTATILKMSSSRSYTRMAGPWGSYELDIPLIGKHNLYNTIQAIAAANTVTSVARKLRPTLEQAKGVPGRLELVRVDEDASEFPAILVDYAHTHDALENVLSAIKPLCEGRLITVFGCGGDRDESKRPKMAKIACDFSDIAIITSDNPRTENPFKIIEQIQAGVPKDTVCDVRVIDDRRHAIEQAIALAGEKDTILIAGKGHEDYQIVGTEKIHFDDRQVAREYLYKGVGTRQ",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must have at least one Mur ligase N-terminal catalytic, Mur ligase C-terminal, Mur ligase central domain.2. The ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate binding site should be located in a region of the protein that is accessible to the ligand.3. Mg(2+) is required for the proper folding and stability of the protein.4. A protein that contributes to cell cycle, regulation of cell shape, peptidoglycan biosynthetic process, cell division by functioning in cytoplasm with magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, tetrahydrofolylpolyglutamate synthase activity.5. Ideal protein should have a binding affinity for key enzymes in the Cell wall biogenesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MILALNTGQLVDGDLYVALPGANAHGGSYVEQARDRGASAVLTDDAGAALVGDTLPTIVVDAPRRRLAGLSAAFYGNPSRKLWMIGVTGTNGKTSVTYFVSKILENAGYKAGLMGTITMKYGEEEIPTRNTTPESYTIQKLFAQMVEKGVNYCVMEVSSHALAQHRVEGCEFDVGVFTNLTQDHLDFHKTMEAYFEAKRKLFTMCKNAVINIDDEYGRRLYREAECQVETFGFSEKADYKASDLKLDVQGTEFQVETPKGSFHVKSPLLGKFNVYNVLAAISVALLEGVSLKDIQQGISSVEGVP",
    "response#2": "MILKEILYKVDVKAVYGDTDILVEMVLDSRAVTQGCLFVAIKGHQVDGRRYIAQAIEHGASAVLAEADCVSQHLSLSVQQNVPIIYFYQLQQQLSQLAGAFYNNPSKNMKIIIGVTGTNGKTSVTYFVSKILENAGYKVGLIGTIEAIIGKKKIESSNTTPESVDLQEYFKEMVEAGCDSVVMEVSSQGLMLHRTQGFVFDYGIFTNLTQDHIGPNEHKDFEDYLNCKKLLFKQCRIGIANIDDEYGKRIFQGHTCAIETYGLSEKADFRVKNIQANNRGTTFNVMAKGRDYEIETEIPGKFSVYNSLTAIAICHHFNVSEENIKRGLKSAKVKGRLEEVPAPGAVRGVVDYAHKPDAIVAVLRALRELATAAGGRVVCVLGAGGDRDAGKRPLMGAAAAAGADLVIVTDDNPRTEDPAAIRAAVLAGTAGGPAQVSEVPGRRTAIAAAVELAKPGDVVALLGKGHEDYQIINGVKHPMDERVLIKEILEER",
    "response#3": "MILEITGIEHDSRKVGKGTLFVCIPGVHVDGHKFIPQAVAAGAQAILTTRSDIEVPEGIAVLQVPELQPALDTIVPFIIEIENICWDSRRVQLNSLFICVKNKNVDRHDYAASAIDNGAIALVIEHDIEKVSKNITIIKVQDSKKAMAKIASLYYNYPSKKIRLIGVTGTNGKTSVTYFVSKILENAGLNTELWGTIKYKIGDKKIPSKRTTPESLDLQKLFWEMKNSGCKYVVMEVSSHALDLKRVDGCEFDTAVFTNLTRDHLDYHKTIENYFKAKLKFFENLKPKVKLINIDDPYGERLIKESNAEVITYSLSPKSDIWANDIRVNSLGTEFILNYKGEKFKIKLKLIGEHNVYNALAAISVSLHLGFSLEEIKRGLEKIEGVKGRFERVDEGQSFTVLVDYAHTPDSLENVLTTIREFAMGKVTCVVGCGGDRDRTKRPQMAQIAASYSEQAIITSDNPRTEDPQQIIDDMMDGVNGFSPERVKSIVDRTEAIRSAIHSASAGDVVLIAGKGHETYQILRDKTIYFDDREVARKVLEG"
  },
  {
    "reference": "MPATLETCLSDLDCASSSSSSSTSDLSGFLTDEEDCARLQQPASATGPPVTVRRGAPGIPGAPDTPRAPDDEQERRRRRGRARVRSEALLHSLRRSRRVKANDRERNRMHNLNAALDALRSVLPSFPDDTKLTKIETLRFAYNYIWALAETLRLADQGLPGGGTRERLLPPQCAPCLPGPPSPASDAESWASGAAASPCAAAASPLSDPSSPVASEDFTYGPGDPLFSFPGLPKDLLHTTPRFIPYH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The presence of BHLH domains is critical for the interaction of the designed protein with its intended targets.2. The protein should exhibit Polar residues compositional bias.3. A protein with DNA-binding transcription factor activity, RNA polymerase II-specific, protein homodimerization activity, chromatin binding, E-box binding functions is required to enable auditory behavior, positive regulation of DNA-binding transcription factor activity, neuron differentiation. The designed protein sequence is ",
    "response#1": "MPGMICKNSDLEFDSLKPCFYPEDDDIYFGGRNSTPPGEDIWKKFELLPTPRLSPGRALAEDSLEPANWATEMLLPEADLWSNPAEEEDIFGLKGLSGSSSNPVVLQDCMWSGFSSREKPETVVSEKLPGGCGSLAVGAGTLVPGAAAATSAGHARSGTAGVGRRKAAWLTELSHLDSECVDSAVIFPANKRESMPVATIPASAGAAISLGDHQGLSSSLEDFLSNSGYVEEGGEEIYVVMLGETQFSKTVTKLPTAAHSENAALTPECAQSGELILKRSDLIQEQHNYAAPPLPYAEDARPLKKPRSQDPLGPLKCVLRPR",
    "response#2": "MPGMICKNSDLEFDSLKPCFYPEDDDIYFGGRNSTPPGEDIWKKFELLPTPRLSPGRALAEDSLEPANWATEMLLPEADLWSNPAEEEDIFGLKGLSGSSSNPVVLQDCMWSGFSSREKPETVVSEKLPGGCGSLAVGAGTLVPGAAAATSAGHARSGTAGVGRRKAAWLTELSHLDSECVDSAVIFPANKRESMPVATIPASAGAAISLGDHQGLSSSLEDFLSNSGYVEEGGEEIYVVMLGETQFSKTVTKLPTAAHSENAALTPECAQSGELILKRSDLIQEQHNYAAPPLPYAEDARPLKKPRSQDPLGPLKCVLRPG",
    "response#3": "MPGMICKNSDLEFDSLKPCFYPEDDDIYFGGRNSTPPGEDIWKKFELLPTPRLSPGRALAEDSLEPANWATEMLLPEADLWSNPAEEEDIFGLKGLSGSSSNPVVLQDCMWSGFSSREKPETVVSEKLPGGCGSLAVGAGTLVPGAAAATSAGHARSGTAGVGRRKAAWLTELSHLDSECVDSAVIFPANKRESMPVATIPASAGAAISLGDHQGLSSSLEDFLSNSGYVEEGGEEIYVVMLGETQFSKTVTKLPTAAHSENAALTPECAQSGELILKRSDLIQEQHNYAAPPLPYAEDARPLKKPRSQDPLGPLKCVLRPR"
  },
  {
    "reference": "PRRRRSSSRPVRRRRRPRRVSRRRRRRGGRRRR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be able to regulate spermatogenesis, chromosome condensation.2. For general function, the protein need meet that They compact sperm DNA into a highly condensed, stable and inactive complex. The designed protein sequence is ",
    "response#1": "KCLILCYELLKQMSISTGLSATMNGIIESLILPGIISIHPVVRNLAVLCLGCCGLQNQDFARKHFVLLLQVLQIDDVTIKISALKAIFDQLMTFGIEPFKTKKIKTLHCEGTEINSDDEQESKEVEETATAKNVLKLLSDFLDSEVSELRTGAAEGLAKLMFSGLLVSSRILSRLILLWYNPVTEEDVQLRHCLGVFFPVFAYASRTNQC",
    "response#2": "TLRKPTMSKVILRTVADKGVHSRVSLAALKKAVSITGYNMAQNTWRFKRVLQNLVKKGMLKQVTGD",
    "response#3": "KCLILCYELLKQMSISTGLSATMNGIIESLILPGIISIHPVVRNLAVLCLGCCGLQNQDFARKHFVLLLQVLQIDDVTIKISALKAIFDQLMTFGIEPFKTKKIKTLHCEGTEINSDDEQESKEVEETATAKNVLKLLSDFLDSEVSELRTGAAEGLAKLMFSGLLVSSRILSRLILLWYNPVTEEDVQLRHCLGVFFPVFAYASRTNQC"
  },
  {
    "reference": "MAPAASSAPRLLRAAMLLLLLVAAGRRAAGAPVVNELRCQCLQTLQGIHLKNIQSVKVTTPGPHCDQTEVIATLKTGQEVCLNPAAPMVKKIIDKMLN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein with growth factor activity, CXCR chemokine receptor binding, chemokine activity functions is required to enable antimicrobial humoral immune response mediated by antimicrobial peptide. The designed protein sequence is ",
    "response#1": "NLKCRCSKVSSTFINLILVDWIQVIRPGNGCPKTEIIFWTKAKKAICVNPTARWLPKVLKFC",
    "response#2": "NLKCRCSKVSSTFINLILVDWIQVIRPGNGCPKTEIIFWTKAKKAICVNPTARWLPKVLKFC",
    "response#3": "DKRCKCVKVTNRPTGLGPIIAVDVIPPGIHCRRTEIIFALKKNRKVCVDPEAPWVQQFIKKLL"
  },
  {
    "reference": "MRTHYCGDLRASDIDKTVTLFGWVDRYRDHGGVIFADLRDRTGRVQIVSDPQRTPESYQAAANLRNEYVIKVEGRVSKRPKESLNPKIPTGEVEIYADSIEILNGVNKQLPFVISSEDAELVREEVRLKYRYLDLRRDRMRKNLQLRHQVVQAMRRYLEDQQNFMEVETPILTRSTPEGARDYLVPSRTNPGKWYALPQSPQLFKQLLMVSGVDRYYQIARCFRDEDNRADRQPEFTQLDMEMSFMSFDEILDLNEGLIAHVFKTVKNIDIPRPFPRLTYAEAMEKYGIDRPDTRFGLELVDVSEIVKDSGFKVFSGAVKSGGIVKVLPIPEATNIISNVQIKPGGELFKEATEAGAKGIAYIRIKDNNELDTIAAIKDNLTDEQKQELIEKTGAKPGHLLLFGAGDTDTVNKSLARLRLVVGEKLGLIDEDKINLLWVTDFPMFEWNAEEKRLEALHHPFTAPKPEDINDLPHARALAYDMIFNGIELGGGSLRIYQRDIQEKVFSTIGLSMEEAYNKFGFLLEAFEYGTPPHGGIAYGLDRFVMLLAKEESIRDVIAFPKTQQASCLLTEAPSTVEPKQLKELHIASTFKPKS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a high affinity for ATP, L-aspartate.2. The designed protein should be involved in aspartyl-tRNA aminoacylation by localizing to cytoplasm and exhibiting ATP binding, aspartate-tRNA ligase activity, nucleic acid binding, aspartate-tRNA(Asn) ligase activity.3. The protein should have a fast turnover rate for ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-   aspartyl-tRNA(Asx).4. For general function, the protein need meet that Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn) The designed protein sequence is ",
    "response#1": "LRLRYQVAMGVRRYLDAQGFIDIETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQIVKCFRDEDLRADRQPEFTQIDLETSFLNEDDIMDITEGMARQVFHDAMDVELGDFPRMTYADAMHYYGSDKPDLRVSLKFAELTDVMKDVPFKVFNAAANLPNGRVVALRVPGGAKLSRKEIDEYTQFVGIYGAKGLAYIKVNDVSKLTTDETSGLQSPIVKNLTEGVLKSIVERTGAENGDIIFFGADKAKVVNEAIGALRIRIGHEHGEAGGYFVREWRPLWVVDFPMFEYDEDDDRWTACHHPFTSPKPGHEDLMATDPGACIARAYDMVLNGWEIGGGSIRIHRADVQEKVFGALKISPEEQQNKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMAGADSIRDVIAFPKTQKASCLLTEAPDTVDAAQLKELYVASKEK",
    "response#2": "SEDVRLKYRYLDLRRPVMFQTFKMRHQVTKAIRDFLDEEGFLEVETPILTKSTPEGARDYLVPSRVHPGEFYALPQSPQIFKQLLMVAGFERYYQIARCFRDEDLRADRQPEFTQVDIETSFMSQDDILAMIERMMARVMKVAKGVDISIPFPRMSYDEAIARYGSDKPDTRFGLELVDLSEQVKDCGFKVFASAVQNGGQVKAINVKGAADKYSRKDIDALTEYVARYGAKGLAWLKVEADGLKGPIAKFFTEDEQKQMMQTLEAETGDLLLFVADKKSVVADALGALRLKLGKDLQLIDESAFHFLWITDWPLFEYDEEEGRYYAAHHPFTMPVREDVPKFETDPASVRAQAYDLVLNGYELGGGSLRIFERDIQEKMFKTLGFTEEQAREQFGFLLEAFEYGTPPHGGIALGLDRLVMLLAGRSNLRDTIAFPKKNEYVGAVVMTGGASQPRRQLDAWQEWAKQRGAKGLAYILVGEDGELSGPVAKNITDAERAGIAAHVGAQPGDCIFFAAGDTKK",
    "response#3": "MPLVKAMRVPGGETMTRKEIDEFTEFVKIYGAQGLAWIKIREGEWQSPIAKFLSEAERAALVAALGLEVGDIVFFQAGEPGMVNAAGFNSSVVVMDPPERLKFRAKVTSAVRSYLEGDGFLDIETPMLTKATPEGARDYLVPSRTHKGKFFALPQSPQLFKQLLMMSGMDRYYQIVKCFRDEDLRADRQPEFTQIDIETTFLGAEQVMKITEGMIRNLFMKMLNVDLGEFPQMTYADAMRRFGSDKPDLRNPLELVDVADILKDVEFKVFSGPANDAKGRVAVIRVPGGASMTRKQIDEYTKFVGIYGAKGLAWMKVNDIDAGLEGLQSPILKFLGEDVAKQVLSRVEAQSGDILLFGADSATVVTEALGALRLKVGEDLDLLEGEWKPLWVIDFPMFEEFDGQFYALHHPFTAPRDMTAEQLAADPANALSNAYDMVLNGCELGGGSVRIHNQDMQAKVFNILGISEEEAEVKFGFLLDALKFGAPPHAGLAFGLDRLVMLMTGATSIRDVMAFPKTTTAACPLTNAPGFASAEQLTELGIRRVAPKAE"
  },
  {
    "reference": "MKKIYLSTGNKGKVSEIKEILSDLTFDVYSKSELGINEDAVEDAETLEENSLIKAKFLKKYTDDIVMSDDTGLFVNSLDGRPGVYSARFAGNECDDSKNRKKLLSELKDKEDRSAYFETVITIIDSNNEIHQAKGRVDGKILLEECGEHGFGYDSIFMPDGYDKSFAQMEDCEKNKISHRKRALENAKIILKGLNESSNN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. The designed protein sequence is ",
    "response#1": "RIFITGMPGVGKTTLALKIAEKLKELGYKVGGFITKEIRDGGKRVGFKIITLDTNEETILAYVGDGKIKVGKYAVFIENLDNVGVEAIKRALKDADIIIIDELGAMEFKSRKFSEVVDEVIKSDKPLLATLHRNWVNKFKDKGELYTLTIENREKR",
    "response#2": "RIFITGMPGVGKTTLALKIAEKLKELGYKVGGFITKEIRDGGKRVGFKIITLDTNEETILAYVGDGKIKVGKYAVFIENLDNVGVEAIKRALKDADIIIIDELGAMEFKSRKFSEVVDEVIKSDKPLLATLHRNWVNKFKDKGELYTLTIENREKS",
    "response#3": "RIFITGMPGVGKTTLALKIAEKLKELGYKVGGFITKEIRDGGKRVGFKIITLDTNEETILAYVGDGKIKVGKYAVFIENLDNVGVEAIKRALKDADIIIIDELGAMEFKSRKFSEVVDEVIKSDKPLLATLHRNWVNKFKDKGELYTLTIENREKL"
  },
  {
    "reference": "MAAAVLTDRAQVSVTFDDVAVTFTKEEWGQLDLAQRTLYQEVMLENCGLLVSLGCPVPKAELICHLEHGQEPWTRKEDLSQDTCPGDKGKPKTTEPTTCEPALSEGISLQGQVTQGNSVDSQLGQAEDQDGLSEMQEGHFRPGIDPQEKSPGKMSPECDGLGTADGVCSRIGQEQVSPGDRVRSHNSCESGKDPMIQEEENNFKCSECGKVFNKKHLLAGHEKIHSGVKPYECTECGKTFIKSTHLLQHHMIHTGERPYECMECGKAFNRKSYLTQHQRIHSGEKPYKCNECGKAFTHRSNFVLHNRRHTGEKSFVCTECGQVFRHRPGFLRHYVVHSGENPYECLECGKVFKHRSYLMWHQQTHTGEKPYECSECGKVFLESAALIHHYVIHTGEKPFECLECGKAFNHRSYLKRHQRIHTGEKPFVCSECGKAFTHCSTFILHKRAHTGEKPFECKECGKAFSNRKDLIRHFSIHTGEKPYECVECGKAFTRMSGLTRHKRIHSGEKPYECVECGKSFCWSTNLIRHAIIHTGEKPYKCSECGKAFSRSSSLTQHQRMHTGKNPISVTDVGRPFTSGQTSVTLRELLLGKDFLNVTTEANILPEETSSSASDQPYQRETPQVSSL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: May be involved in transcriptional regulation..2. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.3. The protein design must be focused on enhancing the efficiency of regulation of transcription by RNA polymerase II. The designed protein sequence is ",
    "response#1": "LMVSLKLDSHRVRFTKYDHTFTSEEAINNLGSLKFSQSNRMPDPKDPSRIVTTTTTTTFSMAKEMARSVCQRFVDARFIESVDGKYSHTFPLKGALYQLTS",
    "response#2": "LMVSLKLDSHRVRFTKYDHTFTSEEAINNLGSLKFSQSNRMPDPKDPSRIVTTTTTTTFSMAKEMARSVCQRFVDARFIESVDGKYSHTFPLKGALYQLTR",
    "response#3": "LMVSLKLDSHRVRFTKYDHTFTSEEAINNLGSLKFSQSNRMPDPKDPSRIVTTTTTTTFSMAKEMARSVCQRFVDARFIESVDGKYSHTFPLKGALYQLTA"
  },
  {
    "reference": "MTNEEKLRGYLKKATNDLLIARARLRELESGEQEPIAIVGMACRYPGGVTSPEELWELANSGGDGISGFPDNRGWDIGGLFDDYPGERGTSYAREGGFLHDAADFDAGFFGISPREALAMDPQQRLLLETSWEAIERAGIDPASLRGSESGVFVGVIYHDYGTSKTVEFPEDVEGYLATGNAGGVMSGRLAYTFGLEGPAVTIDTACSSSLVALHLAGQALRSGECDLALAGGVTVMATPGTFVEFSRQGALSADGRCKSFSDSADGTSWSEGVGILVVERLSDAQRKGHHVLAVVRGTAVNSDGASNGLTAPSGPSQQRVIRQALTAAGLAGVDVDVVEAHGTGTTLGDPIEAQALLATYGQGRPADRPLLLGSVKSNFGHTQAAAGVAGIIKMVMAMRRGVVPQSLHAEVPSSKVDWDSGAVVLATERVPWPDAGRLRRAGVSSFGISGTNAHVIVEQAPESPVAAPVTDTVIRPWVLSGRSPEALRAQAERLLSFVEDSPEIGAASVGRALALARAPFNHRAVVVGTRLADFRAGLASPNLLTGIADGGAKTAFVFAGQGSQRVGMGRELHARFPVFAEAFDAVAAQLELDLLSADEETLGRTEFTQPALFALEVALFRLLESWGVIPDFVAGHSIGEIAAAHVAGVLSLEDACVLVSARGRLMQALPAGGVMVAVQASEADLPPLPDGVSLAAVNGPRSMVIAGTEAAVSQVDGFKSRRLNVSHAFHSALMEPMLEEFRAVIADLSFGQPSIPAVSTVTGAVVT",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have palmitoyl-[acyl-carrier-protein] hydrolase activity, [acyl-carrier-protein] S-malonyltransferase activity, 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity, 3-oxoacyl-[acyl-carrier-protein] synthase activity to facilitate macrolide biosynthetic process, fatty acid biosynthetic process.2. Ideal protein should have a binding affinity for key enzymes in the Lipid metabolism pathway to enhance the overall efficiency of the pathway.3. The protein should be designed to have high stability and activity towards dodecanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxotetradecanoyl-   [ACP] + CO2 + holo-[ACP].4. The presence of Carrier domains is critical for the interaction of the designed protein with its intended targets.5. The protein must exhibit the following characteristics: Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH.6. The protein design should incorporate a binding pocket that specifically recognizes pantetheine 4'-phosphate to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "MEDIVISGISGRYPESDNIDELRENLYNHVDMITEDDRRWPIGIYDLPARLGKIKFLNKFDAQFFGVHGKQATNMDPQARILLELTYEAIADAGINPQFLRGTRTGVYIGASESEVEESLVQDISKADGYALTGCSRSMFANRISYAFDFQGPSYTMDTACSSSFLALQQAFQAIRTNQCDQAIVGGVNICVRPASSLQFHKLNMLSPDGKCHHLDSRANGYVRSESCSVIFLQRKNQAKRIYATIINAKTNTDGHKDEGITFPSYISQRNLMKEVVSEAKINPLDVVYVEAHGTGTMAGDPVETKSIANVYCVGRTQPLLIGSVKTNLGHAEPASGLNSIAKMLVTFENRMIPANLHYKSPNTSIPELINGLVKPVDENTPYDGGIVAINSFGFGGVNVHVLLKPNESPVPKVDGEVFPGQGSQWPGMAVELLESSPVFAARFAQCERALAPFVDWSLTEVLRSGDYDRVDVVQPVLWAVMVSLAALWEAAGVRPDAVAGHSQGEIAAAVVAGALSLEDGARVVALRSRAITVLAGRGGMVSVPLPADEVRALLPEGVAVAAVNGPSSVVVAGDVAGLETVLASVQRARRIPVDYASHSAHVEEIRRQILDLLAPN",
    "response#2": "MEDIVISGISGRYPESDNIDELRENLYNHVDMITEDDRRWPIGIYDLPARLGKIKFLNKFDAQFFGVHGKQATNMDPQARILLELTYEAIADAGINPQFLRGTRTGVYIGASESEVEESLVQDISKADGYALTGCSRSMFANRISYAFDFQGPSYTMDTACSSSFLALQQAFQAIRTNQCDQAIVGGVNICVRPASSLQFHKLNMLSPDGKCHHLDSRANGYVRSESCSVIFLQRKNQAKRIYATIINAKTNTDGHKDEGITFPSYISQRNLMKEVVSEAKINPLDVVYVEAHGTGTMAGDPVETKSIANVYCVGRTQPLLIGSVKTNLGHAEPASGLNSIAKMLVTFENRMIPANLHYKSPNTSIPELINGLVKPVDENTPYDGGIVAINSFGFGGVNVHVLLKPNPPKIPTKDIPWYVFSGMGSQWAGMGRELMVIETFQASLRRCAEALKPVGVDLMNLILNGTDETFESVLNSFVSIAAIQVALVDVLDLVGITPDGIVGHSVGELGCAYADRTFTPEQTVLAAYWRGKSIQEAKLEPGAMAAVGLSWEEAGKKCPEDISPACHNSADSVTVSGPVDSIHKFVAELKKEDIFAKSVNSSGVAFHSKYIAPVGPTLRKNLEKIIEHPKPRSSKWISSSIPESAWGTNVAQYSSAGYHVNNLKFPMPRNFPTFWYVFSGMGSQWAGMGRELMVIETFQASLRRCAEALKPVGVDLMNLILNGTDETFESVLNSFVSIAAIQVALVDVLDLVGITPDGIVGHSVGELGCAYADRTFTPEQTVLAAYWRGKSIQEAKLEPGAMAAVGLSWEEAGKKCPEDISPACHNSADSVTVSGPVDSIHKFVAELKKEDIFAKSVNSSGVAFHSKYIAPVGPTLRKNLEKIIEHPKPRSSKWISSSIPESAWGTNVAQYSSAGYHVNN",
    "response#3": "MEDIVISGISGRYPESDNIDELRENLYNHVDMITEDDRRWPIGIYDLPARLGKIKFLNKFDAQFFGVHGKQATNMDPQARILLELTYEAIADAGINPQFLRGTRTGVYIGASESEVEESLVQDISKADGYALTGCSRSMFANRISYAFDFQGPSYTMDTACSSSFLALQQAFQAIRTNQCDQAIVGGVNICVRPASSLQFHKLNMLSPDGKCHHLDSRANGYVRSESCSVIFLQRKNQAKRIYATIINAKTNTDGHKDEGITFPSYISQRNLMKEVVSEAKINPLDVVYVEAHGTGTMAGDPVETKSIANVYCVGRTQPLLIGSVKTNLGHAEPASGLNSIAKMLVTFENRMIPANLHYKSPNTSIPELINGLVKPVDENTPYDGGIVAINSFGFGGVNVHVLLKSNPKPQPKIPRADVPRLVQVSGRTEAAVNTILQKGKEHSSDEKFLCLLNEIAAVPTAGMPYRGFTLLGANELFSGQGAQRAGMGKELAARFPVFAAALDEVLAHFDQGLREVMFGEADGLDETGFTQPALFAVEVALFRLAESLGVRPDFVAGHSIGEVAAAHVAGVLSLEDACALVAARARLMQALPSGGAMVAVQASEEVVRPRLVDGVSIAAVNGPEAVVLAGDEAEVLRISAELKDEGRKTQRLQVSHAFHSPLMEPMLEEFRRVVQGLSYEAPRIPVVE"
  },
  {
    "reference": "MAVCIRLARGGKKKKPVYSVVVADKRMARDGRFLEKLGQYIPMKEGGTFAINQERYAHWISQGAKPSETVGKLVAKQQNAAEA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that contributes to translation by functioning in ribosome, ribonucleoprotein complex, cytoplasm with structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "GRRLQTIVYKKGLARTIYQARQLITHGHIAISGRKVTSPGYVVLRGEEDLIDYYPTSPFKQNPF",
    "response#2": "MN",
    "response#3": "ERRLQTIVYKKGLARTIYQARQLITHGHIAISGRKVTSPGYVVLRGEEDLIDYYPTSPFKQNPL"
  },
  {
    "reference": "MGGCEVREFLLQFGFFLPLLTAWPGDCSHVSNNQVVLLDTTTVLGELGWKTYPLNGWDAITEMDEHNRPIHTYQVCNVMEPNQNNWLRTNWISRDAAQKIYVEMKFTLRDCNSIPWVLGTCKETFNLFYMESDESHGIKFKPNQYTKIDTIAADESFTQMDLGDRILKLNTEIREVGPIERKGFYLAFQDIGACIALVSVRVFYKKCPFTVRNLAMFPDTIPRVDSSSLVEVRGSCVKSAEERDTPKLYCGADGDWLVPLGRCICSTGYEEIEGSCHACRPGFYKAFAGNTKCSKCPPHSLTYMEATSVCQCEKGYFRAEKDPPSMACTRPPSAPRNVVFNINETALILEWSPPSDTGGRKDLTYSVICKKCGLDTSQCEDCGGGLRFIPRHTGLINNSVIVLDFVSHVNYTFEIEAMNGVSELSFSPKPFTAITVTTDQDAPSLIGVVRKDWASQNSIALSWQAPAFSNGAILDYEIKYYEKEHEQLTYSSTRSKAPSVIITGLKPATKYVFHIRVRTATGYSGYSQKFEFETGDETSDMAAEQGQILVIATAAVGGFTLLVILTLFFLITGRCQWYIKAKMKSEEKRRNHLQNGHLRFPGIKTYIDPDTYEDPSLAVHEFAKEIDPSRIRIERVIGAGEFGEVCSGRLKTPGKREIPVAIKTLKGGHMDRQRRDFLREASIMGQFDHPNIIRLEGVVTKRSFPAIGVEAFCPSFLRAGFLNSIQAPHPVPGGGSLPPRIPAGRPVMIVVEYMENGSLDSFLRKHDG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should contain a Helical membrane-spanning region.2. The presence of Eph LBD, Fibronectin type-III domains is critical for the interaction of the designed protein with its intended targets.3. For general function, the protein need meet that Receptor tyrosine kinase which binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells4. We are looking for a protein with broad substrate specificity for ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-   [protein].5. The protein should be optimized for stability and expression in nucleoplasm where it will participate in axon guidance. The designed protein sequence is ",
    "response#1": "VKIEEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSVPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEERRVLRVFHYFESNNEPATWSSNIRHGDATDVRGIIQKIVDSHKVKNVASYGLRLSHLHSEEVHWLHPDIGVSHIREKYEQSHPPEEWKYELRIRYLPKGFVNQFTEDKPTLNFFYQQVKNDYMSEIADQVDQEIALKLGCLEIRRSYGEMRGNALEKKSNYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRYDKECFKCALGSSWIISVELAIGPEEGISYLTDKGSNPTHLADFTQVQTIQYSSSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVSGAPQIYEKALSSFPDPTLYPLGSRQILTCTVYGIPQPTIKWFWHPCNRNHSKARYDFCSNNEEPFILGPDSNIGNRIESITQRMAIIEGKNKTASTLVVADSRLSGIYSCMASNKVGTVERNIEVTLMDTSTAQGELGWLLDPPETGWSEVQQMLNGTPLYMYQDCPIQEGGDTDHWLRSNWIYRGEEASRVHVELQFTVRDCKSFPGGAGPLGCKETFNLFYMESDQDVGIQLRRPLFQKVTTVAADQSFTIRDLASGSVKLNVEHCSLGHLTRRGLYLAFHNPGSCVALVSVRVFYQ",
    "response#2": "EVTLMDTSTAQGELGWLLDPPETGWSEVQQMLNGTPLYMYQDCPIQEGGDTDHWLRSNWIYRGEEASRVHVELQFTVRDCKSFPGGAGPLGCKETFNLFYMESDQDVGIQLRRPLFQKVTTVAADQSFTIRDLASGSVKLNVEHCSLGHLTRRGLYLAFHNPGSCVALVSVRVFYQRCSCASGWKGLEGEVTLMDTSTAQGELGWLLDPPETGWSEVQQMLNGTPLYMYQDCPIQEGGDTDHWLRSNWIYRGEEASRVHVELQFTVRDCKSFPGGAGPLGCKETFNLFYMESDQDVGIQLRRPLFQKVTTVAADQSFTIRDLASGSVKLNVEHCSLGHLTRRGLYLAFHNPGSCVALVSVRVFYQRCLCPPGFMGKTCSCASGWKGLEGTEEVLLDTTGETSEIGWLTYPPGGWDEVSVLDDQRRLTRTFEACHVAGLPPGSGQDNWLQTHFVERRGAQRAHIRLHFSVRACSSLGVSGGTCRETFTLYYRQADEPDGPDSIAAWHLKRWTKVDTIAADESFPASSSSSSWAVGPHRTGQRVGLQLNVKERSFGPLTQRGFYVAFQDTGACLALVAVKLFSYTCPSVLDKECFKCALGSSWIISVELAIGPEEGISYLTDKGSNPTHLADFTQVQTIQYSSSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVSGASESFIIRPQEVTLMDTSTAQGELGWLLDPPETGWSEVQQMLNGTPLYMYQDCPIQEGGDTDHWLRSNWIYRGEEASRVHVELQFTVRDCKSFPGGAGPLGCKETFNLFYMESDQDVGIQLRRPLFQKVTTVAADQSFTIRDLASGSVKLNVEHCSLGHLTRRGLYLAFHNPGSCVALVSVRVFYQR",
    "response#3": "EEVLLDTTGETSEIGWLTYPPGGWDEVSVLDDQRRLTRTFEACHVAGLPPGSGQDNWLQTHFVERRGAQRAHIRLHFSVRACSSLGVSGGTCRETFTLYYRQADEPDGPDSIAAWHLKRWTKVDTIAADESFPASSSSSSWAVGPHRTGQRVGLQLNVKERSFGPLTQRGFYVAFQDTGACLALVAVKLFSYTVAISSSTTLDCHANGVPEPQITWFKNNHKIQQEPGIILGPGSSTLFIERVTEEDEGVYHCKATNQKCEVLLDTTGETSEIGWLTYPPGGWDEVSVLDDQRRLTRTFEACHVAGLPPGSGQDNWLQTHFVERRGAQRAHIRLHFSVRACSSLGVSGGTCRETFTLYYRQADEPDGPDSIAAWHLKRWTKVDTIAADESFPASSSSSSWAVGPHRTGQRVGLQLNVKERSFGPLTQRGFYVAFQDTGACLALVAVKLFSYTVDKECFKCALGSSWIISVELAIGPEEGISYLTDKGSNPTHLADFTQVQTIQYSSSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVSGASESFIIRPQCSCASGWKGLECSCASGWKGLEGGPYFTVEPEDLSVSPNAPFHMACAAVGPPEPVTIVWWMGDFRVGLPDISPSILNVSGINQSTVFSCEAHNVKGLSSSRTAPSPPHHLTAIGMGAKVELRWKAPQDTGGRTDVTYTVTCEQCWPETGECFPCESSVRYSEPPQDLSSTSLTVSDLEPHINYTFAVEARNGVSGFVTTRSFR"
  },
  {
    "reference": "MKVKGIRKNCLWKWGTMLLGMLMICSAADQLWVTVYYGVPVWKDAITTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLGNVTENFNMWRNDMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTDNLNNGTYGNRTIEKGEVKNCSFKVTTNIKDKMLKEYALFYKLDVVPIDQGNTSYQNYTSYRLLSCNTSVITQACPKVSFEPIPIYYCAPAGFAILKCNNKTFDGKGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSDNFSNNAKIIIVQLNKSVVINCTRLNNNTRKSIPIGPGRAFYATGDIIGDIRKAHCNISGTQWNNTLGQIVKKLREQFSNKTIVFNQSSGGDPEIVMHSFNCGGEFFYCNTTVLFNSTWNGTEGSINAGENDTITLPCRIKQIINLWQEVGKAMYAPPIKGLIRCSSNITGLLLTRDGGNPNSTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRAVGIGAVFLGFLGAAGSTMGAAAVTLTVQARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNTSWSNKSLSQIWDNMTWMEWEREIDNYTELIYSLIEESQNQQEKNEQDLLALDKWSNLWNWFSITNWLWYIRIFIMIVGGLIGLRTVFAVLSIVKRVRQGYSPISLQTRLPTQRGPDRPGGIEEEGGERDRGGSGPLVNGFLALIWVDLRSLCLFSYHQLRDLL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Design the protein with a signal peptide incorporated.2. A protein that contributes to fusion of virus membrane with host plasma membrane, virion attachment to host cell, positive regulation of plasma membrane raft polarization, fusion of virus membrane with host endosome membrane, mitigation of host antiviral defense response by functioning in virion membrane, host cell plasma membrane, host cell endosome membrane, plasma membrane, viral envelope with structural molecule activity.3. The presence of Retroviral envelope protein GP41-like, Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MWVTVYYGVPVWKEATTTLFCASDAKSYEKEAHNIWATHACVPTDPNPQEIELENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDFVKNTTRVNMTSGNTTINTPSTLINVTMNATDTDMKNCSFNITTVLRDKQKKVYSLFYKLDVVQIGENNNSYRLINCNTSAITQACPKITFEPIPIHYCAPAGFAILKCNDRKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIIIRSENLTNNAKIIIVQLNESVTINCTRPNNNTRTGIPIGPGRAFYATGDIVGDIRQAYCTISREQWNKTLQRVATQFRVLLNKTTIVFNSSSGGDAEITTHSFNCGGEFFYCNTSNLFKGTFNGTSNSTGNNENQNITLPCRIKQIINMWQEVGKAMYAPPIAGIIKCSSNITGLLLTRDGGTENNDSETIRPGGGDMRDNWRSELYKYKVVRLEPLGLAPTRAKRRVVEREKRAALGALXLGFLGTAGSTAMGAASLTLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGRHICPTTVPWNSSWSNKSIEEIWGTMTWMEWEREIDNYTELIYSLIEESQTRQEKNEQDLLALDKWASLWNWFNITNWLWYIKIFIMIVGGLIGLRIVFAVLSVVNRVRQGYSPLSFQTLTXHXEXPDRPGRIEEEDGEQGRDRSIRLVSGFLALAWDDLRNLCLFSYH",
    "response#2": "MWVTVYYGVPVWKEATTTLFCASDAKSYEKEAHNIWATHACVPTDPNPQEIELENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDFVKNTTRVNMTSGNTTINTPSTLINVTMNATDTDMKNCSFNITTVLRDKQKKVYSLFYKLDVVQIGENNNSYRLINCNTSAITQACPKITFEPIPIHYCAPAGFAILKCNDRKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIIIRSENLTNNAKIIIVQLNESVTINCTRPNNNTRTGIPIGPGRAFYATGDIVGDIRQAYCTISREQWNKTLQRVATQFRVLLNKTTIVFNSSSGGDAEITTHSFNCGGEFFYCNTSNLFKGTFNGTSNSTGNNENQNITLPCRIKQIINMWQEVGKAMYAPPIAGIIKCSSNITGLLLTRDGGTENNDSETIRPGGGDMRDNWRSELYKYKVVRLEPLGLAPTRAKRRVVEREKR",
    "response#3": "MWVTVYYGVPVWKEATTTLFCASDAKSYEKEAHNIWATHACVPTDPNPQEIELENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDFVKNTTRVNMTSGNTTINTPSTLINVTMNATDTDMKNCSFNITTVLRDKQKKVYSLFYKLDVVQIGENNNSYRLINCNTSAITQACPKITFEPIPIHYCAPAGFAILKCNDRKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIIIRSENLTNNAKIIIVQLNESVTINCTRPNNNTRTGIPIGPGRAFYATGDIVGDIRQAYCTISREQWNKTLQRVATQFRVLLNKTTIVFNSSSGGDAEITTHSFNCGGEFFYCNTSNLFKGTFNGTSNSTGNNENQNITLPCRIKQIINMWQEVGKAMYAPPIAGIIKCSSNITGLLLTRDGGTENNDSETIRPGGGDMRDNWRSELYKYKVVRLEPLGLAPTRAKRRVVEREKR"
  },
  {
    "reference": "MTQHEQRKIVIVGHVDHGKSTLLGRLLMDFGKVPQQTIEKVRKVCESRGVQLEPAFFLDAFQEEQEQGISIDTTRVNFEFDGHRFLLIDAPGHLEFLKNMTSGASGAETGILVIDGHEGIGSQTLRHLKILGILGVGKVVVVVNKMDKSGYKQARFEEVAAEIRAHVEREGQTCLAVVPISALKGENLTSTCEGLSWYTGLPLLPQLVASTSAVSVDMAEAAAAEPLRMVLQDVYRFTDERFYAGRIVSGSITPGARVFFSPSGKVSTVEAIETFPPRDMQLAVKGDSVALRLKDQIFVERGEVVSLPEQTPEVNNELQAKLIWLNSEPLDWANEYLLKVGTQEVSCRLEPSNMVFQQVGNGDFVDVVIKAQKPICFDRNGFGVDKFVICTTYETVACGSIMALTTAASQGSQKSKNIVVESGFVKREEHENRHGHKGTVLWMTGLSGAGKSTLAKALERRLFAAGFRVQVLDGDNLRSGICADLGFEPEDRAENIRRVAHLSKLLLNTGTVVISACISPYEKDRRIAREIIGAEDFHELFIFCPIEVCQSRDPKGLYKKASGGQISDFSGLNAPYQPPANPKLRLDSSTMSIESEVDSIIAMLQAEGIKPPVSAVGARGELAWKP",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Catalyzes the synthesis of activated sulfate..2. The Phosphoserine intermediate active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The protein design should consider the endogenous proteins and enzymes present in the sulfite from sulfate: step 2/3 pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "TMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALESDSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADCPGHEQYTRNMVTGASTADPAVILVDARKGVLVQTRRHSYLVHLLGIRNVVLAVNKMDLVDYDQATFDAIVAEYREFASSIGIEAFTAIPISGFRGDNIRFITCGSVDDGKSTLIGRLLYDSKMIFEDQMTALESDSKKVGTQGGDIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADTPGHEQYTRNMITGASTADVAVILIDARKGVLTQTRRHSYLVSLIGIRKVVLAINKMDLVDYDKAVFERILEDYRAFAARIGLTDITAIPLSALKGVNMLEEAAETPWYHGPSLMGYLETVDVERFLTCGSVDDGKSTLIGRLLYDTKLIFEDQLEALESDSRKHGTAGENIDFALLVDGLEAEREQGITIDVAYRFFATEKRKFIVADTPGHEEYTRNMATGASTADLAVVLVDSRQGILRQTRRHSFIASLLGIRHIVLAVNKIDLIAYDQATFDDIVANYMVFAKDLGFDTIVPVPISARFGDNITTRSENMLWYKGPTLLEHLETVSIRNAID",
    "response#2": "GSVDDGKSTLIGRLLYDSKMIFEDQLASLEADSKRVGTQGGEIDFALLVDGLAAEREQGITIDVAYRFFATEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSYLCHLLGIRHIVLAVNKMDLIGYDQAAYDAIVEDYRAFAESIGITSFTPIVVADTPGHQQVKEIVLAVNKIDVKKELLRFITCGSVDDGKSTLIGRMLYESHMLFDDHLAALEADSKKVGTQDGEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKYIVADTPGHEEYTRNMATGASTADVAIILVDASQGVLTQTRRHSFIVSMVGVKRIILAVNKLDLVDYSEDVFNKIVSDYHNFADEALQLDEITAIPISALKGDNIVGPSENTPWYNGQSIIEYLESVEVSSFRMPVQWVNRPSHDFRGFSGRVVGGTVKPGDPVRVLPAGTTSTVERIVTMDGDLHEAVAGQSVTLTLSDEIDASRGDLCGAA",
    "response#3": "SLLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLASLEADSKRVGTQGGEIDFALLVDGLAAEREQGITIDVAYRFFATEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSYLCHLLGIRHIVLAVNKMDLIGYDQAAYDAIVEDYRAFAESIGITSFTPIPISGFKGDNITGSSPNTIWYTGLEVADQFEAHVVWMHEDEMLPGRPYLMKIGTRTVGVTIAHPKYRVDVNTLDHLAAHTLALNEIGVCNLSLDRAIPFDPYSTNRDTGGFVIIDKLTQNTVGAGFRMPVQWVNRPNLDFRGFAGLIATGTVRPGDAVRVLPSGKTTTIARIVSLDGDLAQAVAGQSVTLTFADEIDCSRGDVIALARELDATVCWFVDAPLRAGDRLALKQTTNTVRATVQELHSRLDPETLEELDQPVDLALNDIGTLTLRTSSVVIADSYTDNRDSGAFILIDETTNDTVGAGTEVSDQFEVTLVWMNDAAMQPGRSYLLKLGAKTVTATVTALKHKVNVNTLEQTTAEHLELNEIGVCTLSLSQPVAFDSYEKNRETGGFILIDRFTNDTLAAGMIRHALRRAQNVHQFQATIVWMDQAELLPGRAYWLKLGTQTVSATVQPPRHAVCVNTMAELSVKTLGLNDIGIAEVYTDRGVVFESYAQGKDLGGFILIDKATNATVAAGMIRHALRRASNVHRQALSVSRERRAAMLGKRPAVFWFTGLSGSGKSTIANLVEKRLHALGCHTYVLDGDNVR"
  },
  {
    "reference": "MVRASDNDKTTHFGYRQVPNSEKPKLVAGVFHSVATKYDLMNDLMSLGAHRLWKRFTIQQSNIREGHRVLDVAGGTGDLARLFAPRVGTEGRVIIADINDSMLSVGRARLADSGIVGNVEFVQADAENLPFADDYFDCITIAFGLRNVTHMERALSSMYRTLKVGGRMLVLEFSHPTRPGLSKIYDAYSFSVLPLLGRLVAKDEVSYRYLAESIRRHPKQNALKAMMTAAGFSNVDYFNLSGGIVALHKAYKL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand.2. The designed protein should be optimized for S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity, 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity, S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity, demethylmenaquinone methyltransferase activity, S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity activity in order to improve aerobic respiration, ubiquinone biosynthetic process, methylation.3. The protein should be optimized for activity in menaquinone biosynthesis, Quinol/quinone metabolism, menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2 pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The protein must exhibit the following characteristics: Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2).. The designed protein sequence is ",
    "response#1": "MTANNTPHPQADFGFQSLPPERKARRVAGVFDSVAARYDLLNSVLSFQVDRYWKRFAIAVSGARPGARVLDLAGGTGDLARAFSRQVGETGEVVLADINASMLREGRDRLLDEGRSTPVLQANAERLPFGSAQFDAVTVAFGLRNMTHKEAALAEMRRVLKPGGRLLVLEFSKVWEPLQPAYDWYSFKVLPRLGQAVAGDAASYRYLAESIRMHPPADELKALLTRAGFARCEYFNLTGGIVALHRGYKL",
    "response#2": "MLDALRYRVESPRLAEGNPAVDVLGFTDDSRQVQPGQLFAAVPGARQDGREFIAEAVSRGAVAVVASEPVTVAGVPWVQVPDVRRALALVAAEFYGEPSRALDLVGAYDTANRVMSMGVDISAEVAARAAAAGREVVVLGRRDAGPDGDRTDAAQLAEALAREGADTVLDFAAAVERVDGRVVGVGGVPVAALLEVLRARGARTVLELAPGDVPVAPRRAGAPVSSAALPAEGSWIHDAGAADDRADDGAAPDPLDAADAAPVVPAAVDPVLRAALAAMPPPVAAPDPAPAGPAPAGPAPAGASAADAADAAPDAPASDAPSAPAPDAPSAPAPDAPSAPAPDAPAATPDLPAAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPVPAAGPAPDAADGAAGAAPAPATVPAPAAVPAPAAAPAPASPAPPAPAAPAAATRASARASSVPALPTTPAPAAAPVPAAAAAPAAAPAPAPAPAPAPAAAVPAPAAAAAPAAPAAPAPVAAPAPTAAAAPAAAAAAPAAPAAPAGPATPAAPAPAAPAAATAPAAPAPAAVAAPAPAAAAAPAPVAAPAPAAAAAPAPAAAPDAAAA",
    "response#3": "MKQTNNTNHANHANHANHANHANHANNANHANPAKSVSSSTKAPASASGNVPRLDDVSLRDLVALLPQAHREVGGDGVAYDLLNRLLSFGVDRRLAVSAAGIFGAPRTRLVAIVGPEASRLLGTPAAEIDADPRRCFALPSLRVALGETAARLYGEPSARLKIIGITGTNGKTTTTTLIRAIFEAAGRKAGLIGTVENVIGDRTIPAAHTTPEASDLQRLLAEMRDAGCTHVVMEVSSHALALDRVRAVRFAGGVFTNLTQDHLDFHGSMEAYGAAKARLFESLPADAFAVVNADDPAARAMAAATRARVLTYGLGPEADVRAEDVRLSLTGTRFTLRTPWGSGEVRSRLVGRFNAYNVLAAVSAAVALGIPLETIRRGIEALESVP"
  },
  {
    "reference": "MDVTKKNKRDGSEVTERIITETVSTRLTSLPPKGGTSNGYAKTGSLSGGSRLEKHSLTHGSSGYINSSGSTRGNASTSSYRRAHSPASTLPNSPGSTFERKIHITRHGTYEGSSSGNSSPEYPRKEFASSSTRGRSQTRESEIRVRLQSASPSTRWTELDDVKRLLKGSRSASVSPTRNSSNTLPIPKKGTVETKMVTASSQSVSGTYDATILDANLPSHMWSSTLPAGSSMGTYHNNVTTQSSSLLNTNAYSAGSVFGVPNNMTSGSSTLHPGVSTCSSVFGMQNNLAPSSSTLSHSTATASTAYGMKKNLPQSPAVVSTGVSTSAACTTNVQNEDLLHKDCKFLILEKDNTPAKKEMELLIMTKDSGKVFTASPASIAATSFSDDTLKKEKQAAYTADTCLVSDANGDVKTVSTKGKAASAEMHNYNHRGGGSGGGGGGGGGGGGGPWGAAPAWCPCGSCCSWWKWLLGLLLTWLLLLGLLFGLIALAEEVRALKARVAELEQSRSNVLLFKEEMQRANKDWLQGEAPSVEAGGKLGLDGHQQEELWLFVRNRLMAEQENGNLRGSPGPKGDMGSQGPKGDRGLPGTPGIPGVLGHPGPQGPKGQKGSVGEPGMEGPMGQRGREGPMGPRGEPGPPGFGEKGDRGAAGEPGVQGPPGVPGSVGPKGSGGSPGPRGPPGPMGPQGLRGEVGLPGIKGDKGPLGSPGPKGDQGEKGPRGLTGEPGLRGLPGAVGEPGAKGAVGPAGPDGHQGPRGEQGLTGMPGTRGP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein localizes to the hemidesmosome. The designed protein sequence is ",
    "response#1": "STCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGDVPPGPLPEAILALYNSTRDRVAGESAETEPEPEADYYAKEVTRVLMVEYGNKIYDKMKSSSHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLTRREEIEGFRLSAHCSCDSKDNTLQVDINGFSSGRRGDLATIHGMNRPFLLLC",
    "response#2": "STCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGDVPPGPLPEAILALYNSTRDRVAGESAETEPEPEADYYAKEVTRVLMVEYGNKIYDKMKSSSHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLTRREEIEGFRLSAHCSCDSKDNTLQVDINGFSSGRRGDLATIHGMNRPFLLLC",
    "response#3": "STCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGDVPPGPLPEAILALYNSTRDRVAGESAETEPEPEADYYAKEVTRVLMVEYGNKIYDKMKSSSHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLTRREEIEGFRLSAHCSCDSKDNTLQVDINGFSSGRRGDLATIHGMNRPFLLLC"
  },
  {
    "reference": "MVDTNPQLPDALYSAAQVRELDAQLIAAGTCGSELMQRAAHATWRALRRRWPEARELSVLAGRGNNAGDGYLIATLAQKAGWNVHVFSVADPATLAGDAADAYGAAITAGLDVQPWADQPLRGIVLDALLGTGLNGDVRQPYAAAIESINASGLPVVAVDIPSGLSADTGRILGVAVRADLTVTYIGLKLGLLTGDAADLVGELVFDDLQADPSAVAKAIRSATRLDTFNLPRLASRPRTAHKGLYGRVLVIGGDHGFGGACLLSAESALRSGAGMVTLATRPEHIPAALARMPEVMSAAIRSANQLMALIEPASVLVVGPGLGQGSWGRSLLSAAANAKRPQVWDADALNQLAQGFVSLPEGSVITPHPGEAGRLLGMTTKQIQADRPAAARALADKYQAVCVLKGSGSLIADVDGQLMLCDRGHPAMATAGLGDVLAGLIGALMAQKMKPFDAACLAVWLHASAGQRLGEAGRGLAASDLIPAIRQLLEEQQPCLS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Mg(2+) is required for the proper folding and stability of the protein.2. The designed protein should have a binding site that is specific to the target (6S)-NADPHX ligand.3. The designed protein should have ADP-dependent NAD(P)H-hydrate dehydratase activity, kinase activity to facilitate nicotinamide nucleotide metabolic process, phosphorylation.4. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. The designed protein sequence is ",
    "response#1": "MRKADASVIRSGTPGVTLMQNAGAAVADAAREMVSPGARIVVLAGPGQNGGDGIVAATRLREQGYRVSLGLLGKREAFSGDAALVAREWPSDVVAVDGANLAPAELVIDALFGGGLSREIEGAARAAIDRINMSGGPVLAVDLPSGIDGETGAVRGIAVRAARTVTFAARKPGHYLLPGRTFCGDVTVADIGVGSSDLQHADVARLLPVPAAESDKYRRGVVGIAAGSARYPGAAVLAVSGALRGGAGAVRYVGPAGDAVIARYPETLVSDQGPAKAGRVQAWVVGPGAGEDSATVAEVLDTDVPVLLDADGLRLADRDAVRGRTAPTLMTPHAGEAAALLGVRREEVEGARLAAVRELAARFRATVLLKGSTTLVADPGGGAVRVNPTGTGWLATAGSGDVLSGLAGSLLAAGLPARDAGSVAAYLHGLAGRFAARGAPVGAHDVAARIPAAWRGVTG",
    "response#2": "MRKADASVIRSGTPGVTLMQNAGAAVADAAREMVSPGARIVVLAGPGQNGGDGIVAATRLREQGYRVSLGLLGKREAFSGDAALVAREWPSDVVAVDGANLAPAELVIDALFGGGLSREIEGAARAAIDRINMSGGPVLAVDLPSGIDGETGAVRGIAVRAARTVTFAARKPGHYLLPGRTFCGDVTVADIGVGSSDLPRDLDGAPWPTPAAAASKYQRGRLLVVAGSDSYRGAALLALLGASASGLGSLRAAVPEAVADQLWSQLPHAVLSARLPSRGDGSLDLSALAPTALERLDALVLGPGIGNTADDAAIWETLQAFPGLLVLDADGLNRLAQCAALPWLRGRSGPSWLTPHPGEFQRLFPDLAGLPPLEACAAAAQQSGCSVLLKGARSVIADPSGERWQLRSASDHAARAGLGDVLAGYAGGLGALAQASGAGCSGSLLALAALAHASAGLQVSTLHGIGAATPHQIAEMLAAMERKLLVVAGSDSYRGAALLALLGASASGLGSLRAAVPEAVADQLWSQLPHAVLSARLPSRGDGSLDLSALAPTALERLDALVLGPGIGNTADDAAIWETLQAFPGLLVLDADGLNRLAQCAALPWLRGRSGPSWLTPHPGEFQRLFPDLAGLPPLEACAAAAQQSGCSVLLKGARSVIADPSGERWQLRSASDHAARAGLGDVLAGYAGGLGALAQASGAGCSGSLLALAALAHASAGLQVSTLHGIGAATPHQIAEMLVASGGATRTGAARLAARAALRIGAGVVTVASPPEALGVNAAQLTAIMLRGCDGPEGLHAILADERLNALVVGPALGVTAVTRAMVAVAAQARRRLVLDADALTSFEGLASELHQGFRSAPTVITPHDGEFTRLFKGHPDILDPSSKVERARRAAAYLSAVAVLKGADTVIAAPDGRAAINANGTPYLGTAGSGDVLCGIIAGLMAQHLPAFEAAAAAVWIHAEAGAAFG",
    "response#3": "MRKADASVIRSGTPGVTLMQNAGAAVADAAREMVSPGARIVVLAGPGQNGGDGIVAATRLREQGYRVSLGLLGKREAFSGDAALVAREWPSDVVAVDGANLAPAELVIDALFGGGLSREIEGAARAAIDRINMSGGPVLAVDLPSGIDGETGAVRGIAVRAARTVTFAARKPGHYLLPGRTFCGDVTVADIGVGSSDLHPEAELFAVEDENWGKAALAPRRSTAHKNDFGHVLAVAGSEGKAGAATLVVLAALRAGAGLVTLAARPEVLRTALPGVPEAMGVPLPGNGPLGPGDLAPLRAALRGKTAFAMGPGIPRGPQTGQLLGELLAALDPGCATVVDADALNALAEERDAIAERLRKAPVRPVLTPHPGEFSRLTGEEVERVESDRIDAAVRASSRFGACVVLKGARTVVADPEGTSAVCAHGNPGMATAGAGDVLTGITAAVLARRSGGGSTGERARLAVFLHALAGDLAARRTGETGLVASDIAEVGLPRVFRRL"
  },
  {
    "reference": "MGGSRLYTGVMSDNPGTTVDFGFRTVGRAEKAGMVREVFDSVAPRYDLMNDLMSLGIHRVWKQIFIGALQPRPTHTLLDLAGGTGDISFGWLKRGGGPVLLTDINNAMLSVGRDRALNRGFANDISFAVMDAEKLPLPDRCVDRVSMAFGLRNCTDKNAVLREARRVLKPGGRFLCLEFSHVTLSALTPIYDAWSFKVLPRLGQMVAGDADSYRYLAESIRTFPDQETLAGMMRDAGLSRVTVRNLTGGIAAIHAGWRL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must be able to demethylmenaquinone methyltransferase activity. The designed protein sequence is ",
    "response#1": "ESPLHIHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWCAEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITALR",
    "response#2": "IHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWCAEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITAL",
    "response#3": "IHLGQVMSRGEKMEFTIQKSIELGVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWCAEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLGPRVLRTETTALTAITAL"
  },
  {
    "reference": "MTKAIGIDLGTTYSCVAVWQNDRVEVIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPHNTVFDAKRLIGRKFDDAEVQSDMKHWPFEVTSVGGKPQIRIEYKGEKKTFTPEEISSMVLLKMRETAEAYLGGTVKDAVVTVPAYFNDSQRQATKDAGIISGLNVMRIINEPTAAAIAYGLDKKTEGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRLVNHFVQEFKRKNKKDLTTNARALRRLRTACERAKRTLSSAAQTTIEIDSLFEGIDFYTSITRARFEELCGDLFSHTIEPVEKVLRDSKIDKGSVHEIVLVGGSTRIPKVQKLLTDFFNGRELNKSINPDEAVAYGAAVQAAILSGDTSEKTQDLLLLDVAPLSMGIETAGGVFTPLIKRNTTVPTKKSEIFSTYADNQPGVLIQVFEGERARTKDNNLLGKFELSGIPPAPRGVPQIEVTFDVDANAILNVSAAEKGTGKSEKITIRNDKGRLSSEQIEEMLKQAEQFAEEDKQALERTQAKNGLESYIYNVRNTTNEPQLKDKLEAADKEALEKIVKEGIEWLDSNTTASTDELKDKQKEIEEQVNPIMTKIYSAAGGAPGGMPGGAPGAAPGGAAPGGDDGPTVEELD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that can perform unfolded protein binding, heat shock protein binding, ATP-dependent protein folding chaperone, misfolded protein binding, ATP hydrolysis activity in nucleus, cytosol, plasma membrane, cytoplasm to support protein refolding, chaperone cofactor-dependent protein refolding, vesicle-mediated transport, cellular response to unfolded protein. The designed protein sequence is ",
    "response#1": "GTVIGIDLGTTYSCVAVMKNGKTEILANEQGNRITPSYVAFTDDERLIGDAAKNQVAANPQNTIFDIKRLIGLKYNDRSVQKDIKHLPFNVVNKDGKPAVEVSVKGEKKVFTPEEISGMILGKMKQIAEDYLGTKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVLRIVNEPTAAAIAYGLDKSDKEHQIIVYDLGGGTFDVSLLSIENGVFEVQATSGDTHLGGEDFDYKIVRQLIKAFKKKHGIDVSDNNKALAKLKREAEKAKRALSSQMSTRIEIDSFVDGIDLSETLTRAKFEELNLDLFKKTLKPVEKVLQDSGLEKKDVDDIVLVGGSTRIPKVQQLLESYFDGKKASKGINPDEAVAYGAAVQAGVLDGSVSKETKKDPLGSFLFLGPTGVGKTELAKALAEDLFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEIEKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTPEGTIPEEVENQVMNILKGRPLGSFLFLGPTGVGKTELAKALAEDLFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEIEKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTPEGTIPEEVENQVMNILKGHFKPEFLNRIDGGTVIGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDTEKHIVVYDLGGGTFDVSLLSIDNGVFEVLATSGDTHLGGEDFDNRVINYLARTYNRKNNVDVTKDLKAMGKLKREVEKAKRTLSSQKSVRIEIESFFNGQDFSETLSRAKFEEINMDLFKKTLKPVEQVLKDSNLKKSEIDDIVLVGGSTRIPKVQELLESFFGKKASKGINPDEAVAYGAAVQAGV",
    "response#2": "GTVIGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDTEKHIVVYDLGGGTFDVSLLSIDNGVFEVLATSGDTHLGGEDFDNRVINYLARTYNRKNNVDVTKDLKAMGKLKREVEKAKRTLSSQKSVRIEIESFFNGQDFSETLSRAKFEEINMDLFKKTLKPVEQVLKDSNLKKSEIDDIVLVGGSTRIPKVQELLESFFGKKASKGINPDEAVAYGAAVQAGVFSGKLSTERPLGSFLFLGPTGVGKTELAKALAEDLFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEIEKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTPEGTIPEEVENQVMNILKGHFKPEFLNRIDEGMDEMEFTEAESNMNDLVSEYQQYQDAT",
    "response#3": "GTVIGIDLGTTYSCVAVMKNGKTEILANEQGNRITPSYVAFTDDERLIGDAAKNQVAANPQNTIFDIKRLIGLKYNDRSVQKDIKHLPFNVVNKDGKPAVEVSVKGEKKVFTPEEISGMILGKMKQIAEDYLGTKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVLRIVNEPTAAAIAYGLDKSDKEHQIIVYDLGGGTFDVSLLSIENGVFEVQATSGDTHLGGEDFDYKIVRQLIKAFKKKHGIDVSDNNKALAKLKREAEKAKRALSSQMSTRIEIDSFVDGIDLSETLTRAKFEELNLDLFKKTLKPVEKVLQDSGLEKKDVDDIVLVGGSTRIPKVQQLLESYFDGKKASKGINPDEAVAYGAAVQAGVFDGKQTKAVLLDVVPLSLGIETVGGVMTKLIPRNTVVPTKKSQIFSTAADNQPTVTIQVFEGERPMTKDNHLLGKFDLTGIPPAPRGVPQIEVTFDIDENGILNVSAEDKGTGKSNKITITNDKGRLSKDEIERMINEAEKFAEEDKKLKERIDARNELETYVYNMKNQIKDKDKLADKLESDDKEKIEAAIKDALEWLEANQLAEVDEFEDKLKEVEAVCNPIISAVYQKSAGGPGGCGAQHEEL"
  },
  {
    "reference": "MAQTVSEVFIDTLLAAGVKRVYGVVGDSLNGLTDVIRRREGIDWMHVRHEEVAAFAAGAEAHLTGELAVCAGSCGPGNLHLINGLYDCHRSRVPVLAIAAQIPTSELGSNYFQETNPQLLFKDCSHYCEFISQPEHAIRVLGIAMRTAIARKGVAVVILPGDVALRACSSAPAFLALDYAPSTTTPPADSLDGAAAILDDSKRVAILGGAGCAGAHDELMEVAGKLKAPIVHAMRGKEFIEPDNPFDVGMTGLLGFASGYHAMMSCDALLMLGTDFPYPQFYPPKAKIIQVDVRGEQIGRRTRVDLGLVGGVKETLAALSPKLAAKADRRYLDDCLENYAKAREGLDDLAVGEPGRKPIHPQFLAREIDALAADDAVFTCDVGTPTIWAARYLHMNGRRRLLGSFSHGSMANALSQAIGAQAASPGRQVVSLSGDGGLAMLLGDLLTLRQLKLPAKVVVFNNGSLGFVELEMKAAGLVDYGTSLVNPDFAKLAESADILGIRVEDPGEVRPALQRAFAHDGPALVDVVVNRQELAMPPTISAAQALGFSLYLLRAVIDGRGGEIVDLAKTNFLPRG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: A peripheral cell membrane enzyme that catalyzes the oxidative decarboxylation of pyruvate to form acetate and CO(2).2. The desired protein should be able to perform a ubiquinone + H2O + pyruvate = a ubiquinol + acetate + CO23. The protein contains novel Thiamine pyrophosphate enzyme central, Thiamine pyrophosphate enzyme TPP-binding, Thiamine pyrophosphate enzyme N-terminal TPP-binding domains that confer a unique function or activity.4. The designed protein must possess lipid binding, pyruvate dehydrogenase (quinone) activity, magnesium ion binding to support pyruvate catabolic process under certain conditions.5. The designed protein should have a high specificity for thiamine diphosphate, FAD binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "TVADVMVAALAASGVRRVYGLPGDSLNGFTDALRRNGAVSWAHVRHEETAAFAAAGEAAVTGRLAVAAGSCGPGNLHLINGLFDAQRSRVPVLALAAHIPRAEIGGEYFQETHPQELFRECSVYREMASLPEQVPRMLRIAMRSALERRGVAVLVVPGEDRLVELLSHSQAVTLLCGSGCAGAHDQVVALADALGAPVVHALRGKEHVEWDNPFDVGMTGLIGFSSGYHAMLNCDTLVLLGTDFPYRQFYPTDACVIQIDRDPQALGRRVPLDLGVVGDVRETLAAIGAVLLGDPDRLVELLSHSQAVTLLCGSGCAGAHDQVVALADALGAPVVHALRGKEHVEWDNPFDVGMTGLIGFSSGYHAMLNCDTLVLLGTDFPYRQFYPTDACVIQIDRDPQALGRRVPLDLGVVGDVRETLAAPIRPEYVAGLVNRLAAEDAVFTVDVGSPVVWAARYLSMNGRRRLIGSFNHGTMACALPLAIGAQTAFRDRQVVALAGDGGLTMLFGELITLTQNRLPVKVIVFNNSSLNFVELEMKAAGIVNFGTDLHNPDFADVATAMGIFGRRVEHPAQLEQAVADALAHDGPAVVDVVTARQELRRRPSAKPIRPEYVAGLVNRLAAEDAVFTVDVGSPVVWAARYLSMNGRRRLIGSFNHGTMACALPLAIGAQTAFRDRQVVALAGDGGLTMLFGELITLTQNRLPVKVIVFNNSSLNFVELEMKAAGIVNFGTDLHNPDFADVATAMGIFGRRVEHPAQLEQAVADALAHDGPAVVDVVTARQEL",
    "response#2": "TVADHVISALALSGVHRVYGLPGDSLNGFTDAIRRAPEMSWEHVRHEETAAFAAAADAALTGELAVCAGSCGPGNLHLINGLYDAQRSRVPVLAIAAHIPRAEIGSEYFQETHPQDLFRECSVYRELVSTPEMLPRILAMAMRAAVEERGVAVVVIPGEVFLRA",
    "response#3": "LEKAATLLNAAKNVTILAGAGVEGAHDEVIALADKLGAPIVHALRGKEFIEYDNPFDVGMTGLLGFASGFRAMEAADALLVLGSDFPYEQFYPDNATTIQVDIRGAQLGKRHPLDLGLVGDVRATSEAISLVLDADKDLRDATEILNSAAKVTILGGAGCNGAHKELIATADALKAPIVHALRGKEYIEYDNPFDVGMTGLLGFSSGYHAMEDCDVLLMLGTDFPYPQFFPSHAKIIQVDLRGNQIGRRTKVDLGLVGSIKDTLSANVADVLVETLAQAGVKRIHGLVGDSLNGITESLRQRKDIEWIHYRHEEAAAFAACAESQLTGELAVCAGSCGPGNLHLINGLYDAQRSRTPVLAIAAHIPSAEIGSGYFQESPSEAVARLAETLNCAKNITLLCGSGCAGAHAEVVKLAETLKAPVVHAMRGKEHIEYDNPYDVGMTGLIGFSSGFHAMMNADTLVLLGTQFPYRAFYPADAKIIQIDINPGSLGSHSHVDMALVGDIKTTLHALLPQLRSGTDRKHLEDSIDHYRKVRKGLDDLADKNPGKTPIHPQYLAQQISEYADDDAIFTCDVGTPTVWAARYLKMNGKRRLIGSFNHGSMANAMPQALGAQSLDKQRQVVALCGDGGFSMLMGDFISVAQLKLPVKLVIFNNSVLGFVAMEMKAGGYLTDGTDLENPDFAAIAAACGIKGMRVEKASELNGVLQEAFAHDGPVLIDAITAKEEL"
  },
  {
    "reference": "MKKILIATKNQDKITEILDLLSDINMKLISFQDIDFNENIEETGRSFQENAVIKALTAGRKTKITTLAEDSGLEIDALGGRPGIYSARYKDGSDMDRCMKILCEMKNISKEKRTARFITIVAIYEPLTEKVVTFEGMSEGYITDKPLGKNGFGYDSIFYNFALKKTNGQAKLAEKNKVSHRSEAILKAKQFLIGL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be designed to have high stability and activity towards dITP + H2O = dIMP + diphosphate + H(+).2. The designed protein should be optimized for XTP diphosphatase activity, nucleotide binding activity in order to improve purine nucleoside triphosphate catabolic process.3. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.4. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP The designed protein sequence is ",
    "response#1": "MILLLATRNKGKAREFEAMLR",
    "response#2": "MDITLILATRNQKKTAEFSEMAR",
    "response#3": "MAPVPRTIVVLSGPSAAGKSTVARALQARSPAVRLAVSATTRAPRPGELDGRDYHFLDAAEFDRQIAAGEFVEHARVHGNRYGTPRAPVEAALAAGQDVLLVIDLEGARQVRQRFPDAVLVFLAPPSVAELERRLRGRGTETPESIARRLRRAADELAMADRYDHVLVNDDLEATVAALEGIVAACG"
  },
  {
    "reference": "MTLTTRFAPSPTGYLHVGGARTALYSWLYAKKNGGDFILRIEDTDLERSTQASVDAIMDGMNWLNLEWTHGPYFQTERFDRYNEAIEQLIASGNAYRCYSTSEEVDAMREEAKAKGEIEKYNGLWRDRTDHPADKPFVIRFKNPLEGDVIIKDMVKGDIAISNGQLDDLIIARSDGTPTYNLTVVVDDWDMKVSHVVRGDDHVSNTPKQINILRALGADVPQYAHIPMILGDDGKRLSKRHGAVGVMQYRDDGFLPEALLNYLVRLGWSHGDQEIFSREEMIELFDLKDCNRAPSGFNTDKLIWVNQHYMKTMDPAYVAEHLAWHMADQGINTENGPALADVVRIQADRVKTLKEMADISRYFYEDFTELDAKAVKKHLRPVVKEPMILVKEKLAALTDWSPEPIHAAINDTAVELELGMGKVGMPLRVAATGGGNSPSLDITLALLDQSKVIERIEQALVVVEARIAAG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a high affinity for ATP. The designed protein sequence is ",
    "response#1": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#2": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#3": "MPELPEVETIRRNLNTVLEGEIIREVLVYYRPIVSNDASFETKLKGQRIHKVEREAKYLKFILDDYLLVSHLRMEGKYFMDAPLNKHIHVVFNLSSGHTLSYEDTRKFGRL"
  },
  {
    "reference": "MRDRSRRSFEFLKLSSLDSGVSSVPFRSATTTSPPGPLPTRAVFPRILSLEEREESRGPIGLPRLRTHTGKHRVSASSRGSRLAVLTPGWNHQRLRWLGREARPFDRAEDTALECGSRETMERPVRVKAWVEENRGSFLPPVCNKLLHQKQLKIMFVGGPNTRKDYHIEEGEEVFYQLEGDMLLRVLERGKHRDVVIRQGEIFLLPAGVPHSPQRFANTVGLVIERRRLKTELDGLRYYVGDTTDVLFEKWFYCEDLGTQLAPIIQEFFSSEQYRTGKPNPDQLLKEPPFPLSTRSVMEPMCLEAWLDGHRKELQAGTPLSLFGDTYESQVMVHGQGSSEGLRRDVDVWLWQLEGSSVVTMEGQRLSLTLDDSLLVPAGTLYGWERGQGSVALSVTQDPACKKSLG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The Fe cation, substrate binding site should be stable and able to withstand changes in environmental conditions.2. The protein design should incorporate a binding pocket that specifically recognizes Fe(2+) to enhance enzymatic efficiency.3. We are looking for a protein with broad substrate specificity for 3-hydroxyanthranilate + O2 = (2Z,4Z)-2-amino-3-carboxymuconate   6-semialdehyde.4. A protein that can perform ferrous iron binding in cytoplasm to support tryptophan catabolic process, quinolinate biosynthetic process, quinolinate metabolic process.5. A protein that is capable of catalyzing reactions within the NAD(+) biosynthesis, quinolinate from L-kynurenine: step 3/3, Cofactor biosynthesis pathway with high efficiency and specificity.6. The designed protein's functionality will benefit from Polar residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "MSEILTANVSNIGADGEAVEDGQRLVVPLRDLGTTEVYPQSLQHSPNGRFVTVCGDGEYIIYTALAWRNKAFGSGTSFAWATDSNTYAVQEGKNKIRAYRNFKERPGLIKSSGSWAVEGVHGGTLLAARGSGFVMFWDWESGAVVRRIEVDATSVSWSGSGELVAISSEDTLYILRFDRGAYDVRADAGEALGDEGVEEAFELVAEVSETIKTSKWVGDCFIYTNTLNRLSYIIGDQSHVINHFDQGVYLLGYLPTHNRIYVAG",
    "response#2": "MGEILTANVSNIGADGEAVEDGQRLVVPLRDLGTTEVYPQSLQHSPNGRFVTVCGDGEYIIYTALAWRNKAFGSGTSFAWATDSNTYAVQEGKNKIRAYRNFKERPGLIKSSGSWAVEGVHGGTLLAARGSGFVMFWDWESGAVVRRIEVDATSVSWSGSGELVAISSEDTLYILRFDRGAYDVRADAGEALGDEGVEEAFELVAEVSETIKTSKWVGDCFIYTNTLNRLSYIIGDQSHVINHFDQGVYLLGYLPTHNRIYVAES",
    "response#3": "MEEILTANVSNIGADGEAVEDGQRLVVPLRDLGTTEVYPQSLQHSPNGRFVTVCGDGEYIIYTALAWRNKAFGSGTSFAWATDSNTYAVQEGKNKIRAYRNFKERPGLIKSSGSWAVEGVHGGTLLAARGSGFVMFWDWESGAVVRRIEVDATSVSWSGSGELVAISSEDTLYILRFDRGAYDVRADAGEALGDEGVEEAFELVAEVSETIKTSKWVGDCFIYTNTLNRLSYIIGDQSHVINHFDQGVYLLGYLPTHNRIYVAGGFSS"
  },
  {
    "reference": "MINFSSFFRIIQNLKKTQWQTLIGPVLILIILSMMVLPLAPFVLDVFFTFNIALSIIILLVSMFTRHTLDFAAFPTILLFSTLLRLALNVASTRVIFLNGHNGTHSAGRVIESFGHFLVGGNFAIGIVVFVILVIINFMVITKGSGRIAEVGARFILDAMPGKQMAIDADLNAGLIGEEEAKKRRLKITKEADFYGSMDGASKFVRGDAIAGILIMVLNIFGGLIIGFFQHNMLLGKAAEVYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTNQNVGEQMISQLFCNSQVILLSAIVLGILGLVPGMPNIIFLVFTVSLLSLSWWLSRKKNNIKKNVLISDSKYKSRENSISEASWNDVELEDPIRIEIGYNLIPMVDIQKKGDLLDKIRIIRKKFAQEIGFLPPLVHIKNNINLKRTSYQIFIKGVEVGQGTCFYGKYMAIRSGRETEPLPFKEVYEPTFGLSGYWIDLNFKNKAEKKGYSVVEASAIIATHLNFLISKHIDELFGRQEAQQLLDHVNLEMPKLTEDLIPNIINLTILHKILKNLLLEEVPIRDMRTILETLSEFSIDQKDPNELTSLVRIALSKIITQKLFYKKDIIEIIGLEKNLEQLLLDNLKGQNNTIEPSLFELLMIKTQEAIEKQKLINAPIVLLVTHPLRFFLSKILRKKFPELTVLSQFEVTDIKKVKMTSIIGS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Required for formation of the rod structure of the flagellar apparatus; Together with FliI and FliH, may constitute the export apparatus of flagellin .2. The protein should be designed to localize specifically to plasma membrane in order to perform its role in bacterial-type flagellum assembly, protein secretion more effectively. The designed protein sequence is ",
    "response#1": "XGXAKXPX",
    "response#2": "LMLLTL",
    "response#3": "LTLGW"
  },
  {
    "reference": "MVNFDLGQVGEVFRRKDKGAIVSGDNPEEEEDVDASEFEEDEVKPVRTKNRRPKEDAFTQQRLAAINPVLTPRTVLPLYLLIAVVFVIVGGCILAQNSKVDEVTIYYQDCMTNATSSWSDIPSEHWQFVFHKYKTYNTAPQWRFVDDESDDFTKQRGTCQIRFTTPSDMKNNVYLNYVLEKFAANHRRYVLSFSEDQIRGEDASYETVHDATGINCKPLSKNADGKIYYPCGLIANSMFNDTFPLQLTNVGDTSNNYSLTNKGINWESDKKRYKKTKYNYTQIAPPPYWEKMYPDGYNETNIPDIQDWEEFQNWMRPGAFDKITKLIRINKNDTLPAGEYQLDIGLHWPVLEFNGKKGIYLTHGSHLGGRNPFLGIVYLIGGCICAAMALILLTFWLFGGRKIADASSLSWNMK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The designed protein localizes to the cytoplasm. The designed protein sequence is ",
    "response#1": "GRTANGKVRLYVIGQGALSAAVAANSKHDIAEAERWNLPIILIVLLAVFGSLAAAAVPLALGVCTVVVTMGLVDLVSMHTIMSVFVTSTVSMFGIALAVDYSLFILMRFREELRSGRQPQEAVDAAMATSGLAVVLSGMTVIASLTGIYLINTAALKSMATGAILAVAIAMLASITLTPAALATFGRAAVKRSVLMHWSQRSECTQSLFWTRWVGWVMHRAR",
    "response#2": "GRTANGKVRLYVIGQGALSAAVAANSKHDIAEAERWNLPIILIVLLAVFGSLAAAAVPLALGVCTVVVTMGLVDLVSMHTIMSVFVTSTVSMFGIALAVDYSLFILMRFREELRSGRQPQEAVDAAMATSGLAVVLSGMTVIASLTGIYLINTAALKSMATGAILAVAIAMLASITLTPAALATFGRAAVKRSVLMHWSQRSECTQSLFWTRWVGWVMHRR",
    "response#3": "GRTANGKVRLYVIGQGALSAAVAANSKHDIAEAERWNLPIILIVLLAVFGSLAAAAVPLALGVCTVVVTMGLVDLVSMHTIMSVFVTSTVSMFGIALAVDYSLFILMRFREELRSGRQPQEAVDAAMATSGLAVVLSGMTVIASLTGIYLINTAALKSMATGAILAVAIAMLASITLTPAALATFGRAAVKRSVLMHWSQRSECTQSLFWTRWVGWVMHRR"
  },
  {
    "reference": "MIEKQTTLKREIIFRGKGLHTGVDVELIVCPAEESSGYVFKRVDLEGQPTIDAIADNVVDTSRGTTIEQNGVRVSTIEHVMAALSGLGIDNALIKINGPEMPIMDGSSRVFVEEIMKVGVETQNAERGFYVIKEKLVYSDPKNGIEIAAYPDESFSIDVLIDYNSRVLGNQYAQLVDLKDFPEQIAPCRTFVFFHELEVLLKNNLIKGGDLQNAIVIMEKEAPQEELDRIADLFNKPRIHVKPEGILNNLDMHFPNEPARHKLLDIIGDLALVGRRIKGKIIAKRPGHHANTELAKIIRKMARKEAMKPNIPKFDLSQPPLLDILQIRNILPHRPPFLLIDKILHMDETTVTGMKNVTMNEDFFVGHFPDEPVMPGVLQIEAMAQVGGIFVLNTVPDPENYVTYFLKIDRVKFKRKVVPGDTIIFRLELLEPIRRGIANMFGQAFVGENLVMEGELLAQIIKTK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a binding site that is specific to the target Zn(2+) ligand.2. The designed protein should be involved in lipid A biosynthetic process, fatty acid biosynthetic process by localizing to cytoplasm and exhibiting UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity, metal ion binding, 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity.3. For general function, the protein need meet that Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.4. Ideal protein should have a binding affinity for key enzymes in the lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 2/6, Glycolipid biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MSKKTIEEAIEDIRQGKMVILVDDEDRENEGDLTMAAQFVTPEAINFMAKYGRGLICLTLTGEKVEKLNLPIMVQDNTSRFQTAFTVSIEAKKGVTTGISAHDRAATILTAIADDAKPHDLVQPGHIFPIRARKGGVLVRTGQTEGSVDLARLAGLKPAGVICEIMNEDGTMARMPDLEIFSEKHGIKIATVADLIAYRMREEKLVREEIAVEKEPVACEQDSFKFKTKKLKHDHLKTEIEALEYQQETEDCPYFNEEK",
    "response#2": "MKFKNIEEAIEDFKQGKFVIVVDDEDRENEGDLVLAAEKVTPEAINFMAKYGRGLICLPLTEDRLNELDLPLMVAENTSSFGTAFTVSIDAKEGISTGISAADRAHTILTAIAKDTKPEDLARPGHVFPLRARAGGVLRRTGQTEGSVDLARLAGFKPAGVICEIMNDDGTMARVPQLEKFSEEHGLKIITIEELIEYRMRTERFVKRIA",
    "response#3": "MSVLSGRINEVLGLIEQGDTIVVVDDENRENEGDFIVAAEMVTPEAINFMITHGRGLVCAPMTEARADELDLPLMVSQNTDYHGTAFTVSVDHVSTTTGISAHERSETIQALVDPSSTPAEFRRPGHMFPLVAKDGGVLRRAGHTEAAVDLARLAGLVPAGALVEIMNDDGTMARLPELMVIGEKFGMKILTIAELIAYRRRNEKLVEA"
  },
  {
    "reference": "MEAKAVARYIRISPRRVRQVIDLIRGKGVREAEAILMVTPKRGSRVVAKVLKSAVANAEHNYDLDASNLVVSRAYVDEGPTLKRYQPRAMGRANIRRKRTSHITVVVGEKED",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein can be expressed in large ribosomal subunit and perform structural constituent of ribosome without triggering undesirable cellular responses.2. The protein must exhibit the following characteristics: The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.. The designed protein sequence is ",
    "response#1": "LPIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#2": "GPIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#3": "EIGCKVTLRGERMYDFLDRLINLALPRVRDFRGVSPQAFDGRGNYSLGIKEQTIFPEIDYDKIDKIRGLEVVVVTTAKTDEEARELLKSMGMPV"
  },
  {
    "reference": "MSILIKNVLLEGKEQNIYITGNTIESISGTGSREKPETEFVIEGKGMAAIPGLFNAHTHAAMTLLRGYADDMPLQEWLETKIWPVEAKLTEQDVYWGTKLACLEMIKSGTTFFNDMYWHWGGSVKAVAETGIRAMLSAVFIDGFDTAKSKEQIKRNKALYEASKKVSQRIIFALGPHALYTVSEESLCWTRDFADKHDLLVHIHLSETTEEVEGCINRYGMRPVEFLDSIEFLNSRTIACHCVHMNRKELGLLKKHDVKIVHNPVSNMKLAAGRLPYEELKELGLYSNIALGTDGCASNNNLDMFEEMKIASLLHKAFSGDPTGMPANEAFELATVNAAKMFRLNSGVIATGKLADVVLLDLKKAELVPNHNLISNIVYSANGSCVDTVICDGKILMADRKVEGEEEIKEKAQEVAYEIVT",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that May also be involved in the recycling of 5'- deoxyadenosine, whereupon the 5'-deoxyribose moiety of 5'-deoxyinosine is further metabolized to deoxyhexoses used for the biosynthesis of aromatic amino acids in methanogens.; Catalyzes the deamination of three SAM-derived enzymatic products, namely 5'-deoxyadenosine, S-adenosyl-L-homocysteine, and 5'- methylthioadenosine, to produce the inosine analogs; The preferred substrate for this enzyme is 5'- deoxyadenosine, but all these substrates are efficiently deaminated; Likely functions in a S-adenosyl-L-methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions2. The protein must exhibit metal ion binding, adenosine deaminase activity.3. The presence of Zn(2+) is necessary for the protein to function in the desired environment.4. The protein should have a high affinity for substrate, Zn(2+).5. We require a protein that can specifically bind to the metabolites produced in the S-adenosyl-L-methionine biosynthesis pathway. The designed protein sequence is ",
    "response#1": "TMPGLVNTHSHVAMTLLRGVGDDEELQTWLNDYIWPKEAKLDEKLVYAGSKLAMAEMIKTGTTTFNDMYFYMEETAKAVEESGIRGVLGYGMIDLFDDEKRKQEIKATKNLIKNSHNTANGRVQVAVAPHAPYTCSKELLSESKKLANKHNLKLHIHVSETQQEVNDLEKQRNQTPFEYLDSIDLLDENTIAAHGVWTTDNEMKLLKEKQVSISHNPSSNMKLASGIAPVSKYIKNDINVAIGTDGVSSNNNLDMFSEMKLTALLQKVNTMNAKTLPAQATFNMATENGARALGINTGSIKEGKLADIVLVNMNVPHMIPVRNPLSNIIYSALGSDVDTVICDGQGVVARQHGQEVAAIPGFINCHTHLAMTLLRGYADDMELMPWLKEKIWPLEARLTEDDIRWGVKLGCLELIRFGITCYNDMYYFPDVTAEATREMGLRAFISGVVFDMKPEMLRDVEPFIRRWKGDELIMPAVGPHAAYTCSEETLLKVKEIADRHDAMVHMHVSETREEVDGFLLTKGMSPVEYLDHLGLLNERLAAVHCVWLSEEDCYLLAERKANVVDCTVSNLKLTSGIAPLNTLMKAGVNVCLGTDGASSNNNLSLFEEMKVTAIVQKNAYHTPAAFSAEQIWQLATENAYRAARLNMGLRAGALADLALIDLRRPWLCPETNMVSHLVYSMAGGVDTTIVNGRGVVARQR",
    "response#2": "MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVITQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADATAFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKIKKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSSQTVEALSQLLTED",
    "response#3": "VLIPGLVNTHTHLPMSLMRGLADDLPLDKWLNDHVWPVESNLEGEHCYAGALLSALEMIKSGTTTCNDMYFFMDEVAQAIDESGIRGLLCHGMIDLFDEDKRKEEYKETLRIIKKCHNTADGRVQVSLGPHTPYTCSPELLNWVRKKADENGLKIHIHVSETEKEVEDSLNDRKKRPFEYLEDLNFLGPDVIAAHSVWLSGAEIALINDNDVKISHNPLSNMKLASGISPVSDMLANGICVSLGTDGVASNNNLDLFQEMKTSSLLQKVRTLDPTVLPASKVLEMATINGATALGMEKETGTIEVGKKADMVLIDMNAPHLTPYRNPISHLVYSAEGKDVNTVICNGEGVMVGMGQKDSFVGDDAPRAVFPSIVGRARRHAMMVGRQTRRAVMWDSKRRWGRCTL"
  },
  {
    "reference": "MQRVIPGQAHALHTAAATRRIEQAAQASLPAHALMQRAGRAVASLALAIAPHARRYWIACGPGNNGGDGLEAASHLLQHGKTPVVTWLGHAGSLPEDARAALDWARAANVVFSGTPPEDCDCAIDALLGLGVTRAPEGQMAEHIAQLNRVRARGLPVLSVDLPSGLHADTGNALGACVQASHTLALLTLKPGLFTAQGRDAAGDIWFEELGISSSPTPSAQAVLNPAPATQARKHASHKGSHGDVAVIGGAPGMAGAAVLAARAALHGGAGRVYVGLLDPGAPLLDALQPELMLRRPETLLNQLDGMTVVCGCGGGEAVGTLLPELLSQASRLVLDADALNAVAKDPALRMLLETRGQSGGALTTVLTPHPLEAARLLNTDVHGVQTDRLTAAQMIADRWGCTVVLKGSGTVISTPGAVPRINPTGNGRLATAGTGDVLAGLIGARLVRDTTAFDAACYAVYTHGWVADNWPPRQTLTAGELARALDA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a high specificity for AMP, K(+), (6S)-NADPHX binding, with minimal off-target effects.2. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.3. A protein with ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding, metal ion binding, NADPHX epimerase activity, NADHX epimerase activity is needed to enhance nicotinamide nucleotide metabolic process in a specific context.4. The protein have enhanced (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.5. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.6. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. The designed protein sequence is ",
    "response#1": "MMITDINCECLGLSRLCLMESAGKSLAEEVGKIAVYTFSKPVKIAIFTGSGGNGGDGFVAARYLLNRGYDVDIYMLTDKDNIHSDNAKTNFEIITNMEPRLSHLTVHYLDTIEDVNNCDIAQSESFSEFIIVDAILGTGIKGELRPKVKRAVEVINKSNGLTVSVDVPSGMDPLTGEVSDVAVEPHYTISFHRVKTGIHLAGEDKVGGIVTCDIGIPIEAEEVLPAREQHTHKGKQGRALLVGGNKGFGGAILIASQACARVGAGLTSVITHEDYLNSVILHQPEVMAHTYNPIESNTLFEMADVIAIGPGLGKDEWAERLLKRTLNENCFKVLDADALNILAENPILIESLDTNTLITPHPGEAARLLDCSTTDIEEDRIKAIKALRAKLNCHVTLKGSGTLITHPNTDLITVCRYGNPGMAAGGMGDALTGIAAALIAQTYQNQSSINDVIELAVCLHSHAADEEAAAHGERGLLASDLADRARHIVLTVAGSVNYSGAAYLACSGAMRAGAGLVTLAVPVNLQPVLAGKLNEVTYLPLSESVKGVVSGEASFIVLSSLHEYTTLLIGCGLGQHDMTVSFTKGILFGNKPIMPLVIDADALNVLAKTNDWWQKLMMDAILTPHPGEMSRLTGIKIEEIQSNRIDTAMLFAQKWGKTVVLKGAYTVIASADGRCRVSPFANPGMASAGTGDVLAGIIAGLTAQGLDNFNAACLGVWLHGQAGEIVKAELGDTGMLAGDLLQVVEPALWLPEYPWPTVEGHKYLRGHAVVASGPIYSTGAARLAARGALRAGAGLVTVASPRDALAVNAAQLTAIMVREADDARGLAALLADTRKNAVLIGPGHGVGEGTRAMVLAALAAGPRVVLDADALTSFADDPETLFKAIHASEAPVVMTPHEGEFARLFGDLIAEPKVERTRKAAARAGATVLLKGADTVIAAADGQAAINGTASPWLATAGSGDVLGGIVLGLLAQGMTGFLGASAAVWMHGEAAKTFGPGLIAEDLPEMLPAVLRGLE",
    "response#2": "MMITDINCECLGLSRLCLMESAGKSLAEEVGKIAVYTFSKPVKIAIFTGSGGNGGDGFVAARYLLNRGYDVDIYMLTDKDNIHSDNAKTNFEIITNMEPRLSHLTVHYLDTIEDVNNCDIAQSESFSEFIIVDAILGTGIKGELRPKVKRAVEVINKSNGLTVSVDVPSGMDPLTGEVSDVAVEPHYTISFHRVKTGIHLAGEDKVGGIVTCDIGIPIEAEEHLPRRPLDSHKGTFGHVLVVAGSRNYVGAAYLASQASVRAGAGLTTLATPESVYPVAAAKATEPIHMPLPEDEEGRVGAQAAESLRRGSRRYTNILVGCGLGLSEGTAKFVEDLLFGGEGSGLSGLPALVDADGLNNLARIAGWPERGRCHMVLTPHPGEMATLMGLQASEIQKHRVLVAREYAAKWNVCLVLKGANTVIGEPGGMVRVAPFANPGMATGGTGDVLSGVIAGLMAQGLAPAVAACCGVYLHGEAGRETASRLGNMGVAASDLLPEIPMAVVLVVAGSRNYVGAAYLASQASVRAGAGLTTLATPESVYPVAAAKATEPIHMPLPEDEEGRVGAQAAESLRRGSRRYTNILVGCGLGLSEGTAKFVEDLLFGGEGSGLSGLPALVDADGLNNLARIAGWPERGRCHMVLTPHPGEMATLMGLQASEIQKHRVLVAREYAAKWNVCLVLKGANTVIGEPGGMVRVAPFANPGMATGGTGDVLSGVIAGLMAQGLAPAVAACCGVYLHGEAGRETASRLGNMGVAASDLLPEI",
    "response#3": "MGYGDLLRLKNRPSKSHKGNNGKVLIVGGSKDYSGAPTIAGLSAISSGADLVYVAAPESASLAIKSTSPDLIVRSLEGDYLTSQHAEEILELVEEVDAVLLGPGASLNDETAKLFNILATKIKKPLVIDADALKLVDINLIKNNENVILTPHLFEFKTFFADKIKELAIDESKLKLSLAGTEFSSIDENISMFQQITKAIKGTVIIKGEYDLILSGNKFKINKTGNPGMTVGGTGDALSGLATSLLSQGLDTFNAAALAIYINGRAGDVAMNKQGYGFSATDLIGYIGAIMVAPSLFVERFPSRSFASHKGTYGSLAVVGGDTGMCGAPILAARAALHTGAGKVHVAFIGEGAPPYDPPFPELMLHPPGTLGFGLEAMDALAIGCGMGHGPRAVSSLKQAIASDLPLLLDADALNLLASETALADALAARAVIDVDGNADAESGGTACACILTPHPLEAARLMGCDAAQVQRDRIGTARALAARFGAVVVLKGAGTVIASPDGRAAVNPTGNAGLATGGTGDVLGGIVGALLAERLPSYEAALAGVYLHGWAADTLTASGRGPAGLTAGELAPVVRDLLPESDKYRRGVVGVAAGSERYPGAAVLAVAGALRGGAGAVRYVGPPAEAVLARFPEVLIGPGRVQAWVAGSGLGDAPEARERLARALDDGVSTVVDADGLRMLGPAPGRTARGPLVLTPHAGEAAALLGVAREEVEEARLESVRQLARQTGATVLLKGSTTLIASPPGEADAGEEDGGTVLANLNGTGWLATAGTGDVLAGLIGSLLAAGLAPADAAAVGAHLHGLA"
  },
  {
    "reference": "MHHPFGKEETASQKQLFGFFCECLRRGEWELAQACVPQLHEAQGDIPKKVEDILQALVVCPDQLRCGQDIDPQRLAWVWFLVLEKWFSQEKKLLPSVFRRKLEFLLLSEDLRRDIPEDILKELYEALAQDTGGPVLDGNQKRESWTPRLSSEAVSVFWDLLRQAPQLAQALLELLRTKDDSAGLSGWSLQKALVDRVRRALGAVQGPTTGPAGIVDAIYGALRSLRCPAEPLGGELRLLCEELLEACRAEGSPLQEEQVLSCLLHKAGRSLVSLYGHIYAEKATEKPAKAVHLGKVSPDHLDPEQAMLALFCNPDPSQAWKSAYFYCLSNSKHFLEQILVTALALLKEEDFPSLGCLLDREFRPLSRLLVLLGWMHCQSLASAKRLLQTLHRAQDQGCDKLLRDACDGLWAHLEVLEWCVQQSSNPIPKRDLLCHLHGGDSHSVLYSLHHLTNLPALREEDVLKLLQKVPAKDPQQEQDSAEAPVPEHLSRCQNLTLYQSFCAMKYAIYALCVNSHQHSQCQECRDSPSEDPALAAEPANDSLSSPGASDLFSTYLARCQQYLCNVPDSLCLELLENIFSLLLITSADLHPEPHLPEDYAEDEDIEGKGLLGLRSPSESPQHIAQPERKSEQGCQEVPRSLACTVPNCLKTEPKESSPGLHGHSFLDLKHFTSGISGFLADEFAIGAFFRLLQEQLDKLSSHSPPEKPKLPEGQSCSGSRDGLQSRLHRFSKVLSEAQWRYKVVTSNQGSEEQPSRRYWPIATGHPSL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. Incorporate a signal peptide in the protein design.2. Prioritize Pro residues compositional bias within the protein sequence to achieve desired characteristics.3. For general function, the protein need meet that Phosphatidylinositol 3-phosphate-binding protein required for the abcission step in cytokinesis: recruited to the midbody during cytokinesis and acts as a regulator of abcission4. A protein that contributes to autophagosome organization, regulation of cytokinesis by functioning in late endosome with phosphatidylinositol-3-phosphate binding. The designed protein sequence is ",
    "response#1": "LMGFIKLKLNRYAIIANTVEETGRFNGHVFYRVLQHSIVSTKFNSRIDSEEAEYIKLLELHLKNSTFYFSYTYDLTNSLQRNEKVGPAASWKTADERFFWNHYLTEDLRNFAHQDPRIDSFIQPVIYGYAKTVDAVLNATPIVLGLITRRSIFRAGTRYFRRGVDKDGNVGNFNETEQILLAENPESEKIHVFSFLQTRGSVPIYWAEINNLKYKPNLVLGENSLDATKKHFDQQKELYGDNYLVNLVNQKGHELPVKEGYESVVHALNDPKIHYVYFDFHHECI",
    "response#2": "LMGFIKLKLNRYAIIANTVEETGRFNGHVFYRVLQHSIVSTKFNSRIDSEEAEYIKLLELHLKNSTFYFSYTYDLTNSLQRNEKVGPAASWKTADERFFWNHYLTEDLRNFAHQDPRIDSFIQPVIYGYAKTVDAVLNATPIVLGLITRRSIFRAGTRYFRRGVDKDGNVGNFNETEQILLAENPESEKIHVFSFLQTRGSVPIYWAEINNLKYKPNLVLGENSLDATKKHFDQQKELYGDNYLVNLVNQKGHELPVKEGYESVVHALNDPKIHYVYFDFHHECK",
    "response#3": "LMGFIKLKLNRYAIIANTVEETGRFNGHVFYRVLQHSIVSTKFNSRIDSEEAEYIKLLELHLKNSTFYFSYTYDLTNSLQRNEKVGPAASWKTADERFFWNHYLTEDLRNFAHQDPRIDSFIQPVIYGYAKTVDAVLNATPIVLGLITRRSIFRAGTRYFRRGVDKDGNVGNFNETEQILLAENPESEKIHVFSFLQTRGSVPIYWAEINNLKYKPNLVLGENSLDATKKHFDQQKELYGDNYLVNLVNQKGHELPVKEGYESVVHALNDPKIHYVYFDFHHECK"
  },
  {
    "reference": "MLPHFEASLDFAKQLDKLDPLSDFKNLFHFPQHKGQTAIYFCGNSLGLQPKSVAAAVETELTTWKELAVGGYFNGPNPWLHYQEYLKPSLAKLVGAKELEVSAMNALTVNLHLLMLSFYKPTKDRFKIIMEAGAFPSDQYAVETLVKHFGLNPSEAIIEIAPLEGKKTLSTDQIINTIEEAGDILSMVLFGGINYYTGQFFDLKAITHAAHKVGAIAGFDLAHVTGNIPLSLHEWEVDFAAWCSYKYLNAGPGAVGGFFIHEKHAVNPSIPRLAGWWGNDEKERFKMEKGFIPKTNASGWNISTAQVFNMVGLKASLSIIELADIYRIREKSIVLTAYLAYLLNQLDRISFEIITPEDKDQRGAQLSLYFKERAKEIHQKLIDNGVIVDYREPGVIRVAPAPLYCSFEDVFKFYEILKTNF",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should take into account the physiological conditions of the NAD(+) biosynthesis, quinolinate from L-kynurenine: step 2/3, Cofactor biosynthesis pathway.2. We need a protein that can perform H2O + L-kynurenine = anthranilate + H(+) + L-alanine with high efficiency.3. The designed protein should have 3-hydroxykynureninase activity, kynureninase activity within cytoplasm to regulate anthranilate metabolic process, L-kynurenine catabolic process, tryptophan catabolic process, 'de novo' NAD biosynthetic process from tryptophan.4. The protein must exhibit the following characteristics: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively..5. The designed protein must have at least one Aminotransferase class V domain.6. The pyridoxal 5'-phosphate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MIGETIAIVWLPLVQYYSGQLFDIPPVAAKTHEIGALIGLDMAHGIGNVECKLDEWEVDFAVWCTYKYLNSGPSGIGGYFIRTGLDDGGRRLAGWWGNESATRFQMAPTFRPTPGAKGYQHSCTPVLSSIPLLATLELIDKAGFSNMIAKGRRLTNTMEELLKSSRISGETIGSGETIAIVWLPLVQYYSGQLFDIPPVAAKTHEIGALIGLDMAHGIGNVECKLDEWEVDFAVWCTYKYLNSGPSGIGGYFIRTGLDDGGRRLAGWWGNESATRFQMAPTFRPTPGAKGYQHSCTPVLSSIPLLATLELIDKAGFSNMIAKGRRLTNTMEELLKSSRISGETIGSRIGETIAIVWLPLVQYYSGQLFDIPPVAAKTHEIGALIGLDMAHGIGNVECKLDEWEVDFAVWCTYKYLNSGPSGIGGYFIRTGLDDGGRRLAGWWGNESATRFQMAPTFRPTPGAKGYQHSCTPVLSSIPLLATLELIDKAGFSNMIAKGRRLTNTMEELLKSSRISGETIGSRIIGSRIVGSRIIGSRIIGSRIIGSRIIGSRIIGSRIIGSRIIGSRIIGSRIIGSRIIGSRIVGSRIVGSRIVGSRIVGSRIVGSRIVGSRIVGSRIVGSRIVGSRIVGSRIVGSRIVGSRIAGSRIVGSRIVGSRIVGSRIVGSRIVGSRIVGSRIVGSRIVESRIVESRIVES",
    "response#2": "MILDNWAKLGVDAHHAAGTPWYSYHEALREPTARLVGAKPTEVVCMNSLTVNLHLMMATFYRPTKSRFKILMEEPAFPSDTYAIKTQIVHHGLDAKDALILARPRKGEFTIRTEDILDLIKKHADQLAVVMFGGVNFFTGQLFDVKKIIAAAREHGIIVGFDLAHAIGERIGETIAIVWLPLVQYYSGQLFDIPPVAAKTHEIGALIGLDMAHGIGNVECKLDEWEVDFAVWCTYKYLNSGPSGIGGYFIRTGLDDGGRRLAGWWGNESATRFQMAPTFRPTPGAKGYQHSCTPVLSSIPLLATLELIDKAGFSNMIAKGRRLTNTMEELLKSSRSSIASIDDLDAGETIAIVWLPLVQYYSGQLFDIPPVAAKTHEIGALIGLDMAHGIGNVECKLDEWEVDFAVWCTYKYLNSGPSGIGGYFIRTGLDDGGRRLAGWWGNESATRFQMAPTFRPTPGAKGYQHSCTPVLSSIPLLATLELIDKAGFSNMIAKGRRLTNTMEELLKSSRSSIASIDDLDALGETIAIVWLPLVQYYSGQLFDIPPVAAKTHEIGALIGLDMAHGIGNVECKLDEWEVDFAVWCTYKYLNSGPSGIGGYFIRTGLDDGGRRLAGWWGNESATRFQMAPTFRPTPGAKGYQHSCTPVLSSIPLLATLELIDKAGFSNMIAKGRRLTNTMEELLKSSRSSIASIDDLDALGETIAIVWLPLVQYYSGQLFDIPPVAAKTHEIGALIGLDMAHGIGNVECKLDEWEVDFAVWCTYKYLNSGPSGIGGYFIRTGLDDGGRRLAGWWGNESATRFQMAPTFRPTPGAKGYQHSCTPVLSSIPLLATLELIDKAGFSNMIAKGRRLTNTMEELLKSSR",
    "response#3": "MGETIAIVWLPLVQYYSGQLFDIPPVAAKTHEIGALIGLDMAHGIGNVECKLDEWEVDFAVWCTYKYLNSGPSGIGGYFIRTGLDDGGRRLAGWWGNESATRFQMAPTFRPTPGAKGYQHSCTPVLSSIPLLATLELIDKAGFSNMIAKGRRLTNTMEELLKSSRRLRKIAGRDVAFLGPNIGLLSASESNEFGVLLDPVLREMYFEAVRCSAKAFEIAMRFGCNAIKVAKRHSFAAGYAMADYCKDVGLNCVDVGLGASEAVWKAYNVGASFVKVVSAELGGSLSVLEAIRSPINKKLVVMASGGIRTAEDAAKCIFAGADIVMVGKALLKAENPRQAIKEILG"
  },
  {
    "reference": "MSLTRCPECRKKISENAENCPNCGFSFKQKDLEMYKQRLEARRLHNEEVNRKSTKLHIIWFCIFAIFIAVTSWMVN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The designed protein should be expressed specifically in the membrane The designed protein sequence is ",
    "response#1": "GNY",
    "response#2": "MYRP",
    "response#3": "GWP"
  },
  {
    "reference": "MLSLQTLAKKAVAKQSVPEEYHYILKYCGLWWQNKPISLCHYCNYVILSSTPFKGELLHLDVALIMAIKENNYDVIRLFTEWGANIYYGLTCARTEQTQELCRQLGAKDSLNNKEIFTGLMRHKTSNNIILCHEIFDKNPMLETLNVQEMGEEIHRELKLFIFYILDNVPMNVLIKYWYAIAVKYKLKRAISFFYQTYGHLSMWRLMCAIYFNNVFDLHEIYEQKIVHMDIDKMMKLACMQDYNFLTIYYCFVLGGDIDKAITVTQWHHQTNNLYFCKDLKDLKQNILTARPLLLPNITDPKKIYTMLKNYLPTSSNSL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Plays a role in virus cell tropism, and may be required for efficient virus replication in macrophages.2. The protein design should aim to mimic or enhance the function of natural proteins involved in taxis, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "IHGAYTHVHSSVQRGIRGLGNLLFHSTLFPGGQTQGALTGLYATEPALGPRAHSRFRRIFAKGVQQAEMLQGAGVPTLGGFLKTVRTIATTPGNALAVCSISTTTSKECISLRRMIPQQTVVCLGRFEPTDGPDTYPNLYRDSSDNAVRILETLKLVQRLAKGPIFSGLNRSHDPAPVVRHLQALAPRTGLELFVSKLPDEVRAHLPADPAAGPDAVEAAVAEHFLNVYCSLVFAVVAESGAVPGDLGETPLEVLQRAARLCACQITVLGRTSEQPGC",
    "response#2": "IHGAYTHVHSSVQRGIRGLGNLLFHSTLFPGGQTQGALTGLYATEPALGPRAHSRFRRIFAKGVQQAEMLQGAGVPTLGGFLKTVRTIATTPGNALAVCSISTTTSKECISLRRMIPQQTVVCLGRFEPTDGPDTYPNLYRDSSDNAVRILETLKLVQRLAKGPIFSGLNRSHDPAPVVRHLQALAPRTGLELFVSKLPDEVRAHLPADPAAGPDAVEAAVAEHFLNVYCSLVFAVVAESGAVPGDLGETPLEVLQRAARLCACQITVLGRTSEQPGR",
    "response#3": "IHGAYTHVHSSVQRGIRGLGNLLFHSTLFPGGQTQGALTGLYATEPALGPRAHSRFRRIFAKGVQQAEMLQGAGVPTLGGFLKTVRTIATTPGNALAVCSISTTTSKECISLRRMIPQQTVVCLGRFEPTDGPDTYPNLYRDSSDNAVRILETLKLVQRLAKGPIFSGLNRSHDPAPVVRHLQALAPRTGLELFVSKLPDEVRAHLPADPAAGPDAVEAAVAEHFLNVYCSLVFAVVAESGAVPGDLGETPLEVLQRAARLCACQITVLGRTSEQPGR"
  },
  {
    "reference": "MIIVTGGAGMIGSNIVKALNEAGINDILVVDNLKNGKKFKNLVDLDITDYMDRDDFLTQIMAGDDFGPIEAIFHEGACSATTEWDGKYMMLNNYEYSKELLHYCLDREIPFLYASSAATYGETETFVEEREYEGALNVYGYSKQQFDNYVRRLWKDAEEHGEQLSQITGFRYFNVYGPREDHKGSMASVAFHLNNQINAGENPKLFAGSESFKRDFVYVGDVCKVNLWFLENGVSGIFNCGTGRAESFEEVAKAVVKHHNKGEIQTIPFPDHLKGAYQEFTQADLTKLRAAGCDVEFKTVAEGVAEYLAIQNR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a high specificity for NADP(+) binding, with minimal off-target effects.2. The protein must have high specificity towards ADP-glyceromanno-heptose 6-epimerase activity.3. For general function, the protein need meet that Catalyzes the interconversion between ADP-D-glycero-beta-D- manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose. The designed protein sequence is ",
    "response#1": "MTNGCFDILHPGHVSYLENARKLGDRLIVAVNCDQSVKRLKGESRPINSLSARMAILAGLASVDWVIAFEEDTPQRLISEILPDVLVKGGDYKPEQIVGCQEVWANGGNVKALNFEDGCSTTDVIL",
    "response#2": "IVTGAAGFIGSNLVKALNERGEQRIIAVDNLTRADKFKNLVDCEIDDYLDKTEFVERFRRGDFGKVRAIFHEGACSDTMETDGRYMMDNNFRYSREVLDVCLAQNIQFLYASSAATYGGSSRFVEEREVEQPLNVYGYSKFLFDQVIRRVLPTAKSQIAGFRYFNVYGPRETHKARMASVAFHNFNQFRAEGKVKLFGEYNGYAAGEQTRDFVSVEDVVKVNLFFFDNPDKSGIFNLIVVTGAAGFIGSNLVRGLNRRGIYDIIAIDDLTEGDKFRNLVDCRIADYMHHEDARALLKAGQFPRVRAVLHQGACSDTTERNGQYMMDNNYRVTLEWFEYCQANRVPLIYASSAAVYGASTVYVEDPANEGPLNVYGYSKLLFDQVLRTRMDKLTAQVVGLRYFNVYGPHEQHKGRMASVAFHNMNQFLAEGHVRLFSGWDGYADGGQSRDFISVEDVVDVNLHFLDHPGTSGIFNCE",
    "response#3": "MTNGCFDILHAGHVSYLANARKLGDRLIVAVNSDASVKQLKGPSRPINPLMQRMIVLGALDSVDWVVPFEEETPQRLIASILPDVLVKGGDYKPEDIAGGKEVIEAGGEVKVLNFEDGCSTTNIIDA"
  },
  {
    "reference": "MDDQVPGVAGVVVLAAGAGTRMKSRTSKLLHQIAGRSLLSYAIHAAEFLDPERLVVVVGHEREQIEAHLADIAPQVTVAVQEQQNGTGDAVRAGLEHLADVEGEIVVTYGDVPLLTGETLAALVAAHRTHSDAVTVLSTVLDDPSGYGRIVRDADGGQVAAIVEQKDATPQQAAITEINAGIYVFDAATLRAGLASLTSNNAQGELYLTDVITFARERGRDVHAMILEDRWQAEGVNDRIQLSRLGREQNRRLLEKWMRAGVTILDPETTWVHDTVDLAPDVTLLPGTHLEGATSVATGATIGPDTTLIDVEVREDATIVRTHGSLAIIGAGASVGPFAYLRPGTELGDRGKIGTFVETKNAMIGEGAKVPHLTYAGDVEIGPDANIGAGTIFANYDGVTKSRSVVGKASFVGSNSVIMAPVEIADGAYVGAGSAITDPVGPGELAVARARQRNIAGWVAARRAGTKTFTAAREASEGTPEQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should consider the endogenous proteins and enzymes present in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway to avoid interference with native metabolic processes.2. The ideal protein would be one that plays a critical role in peptidoglycan biosynthetic process, lipid A biosynthetic process, regulation of cell shape, cell morphogenesis, cell wall organization through UDP-N-acetylglucosamine diphosphorylase activity within cytoplasm.3. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.4. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc)5. The protein requires Mg(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "VVVLAAGAGTRMRSKTPKVLHSLAGRSMLAHALHAANEIDPAHLITVIGHDREQVGAAVAAVAAELGRDITSAIQEEQLGTGHAVQCALTAVPADFSGDLLVTSADVPLLDGHTLSALLDEHRSYPERSAVTVLTFVPEDANGYGRIVRDADGHVVEIVEHADATPEQAALTEVNSGVYVFDAAVLRTMVHRLSTANAQHELYLTDVLKLAREAGHPVHGETEGVNDRVQLARAELAQAEAAFQRCARLRLMEDGVALQAPETVWLAWDTVIGRDAEIEPNVVFGPCVTIESGARIRAFSHLEGCHVSRGAIVGPFARLRPGAELAEHSRVGNFVEIKNAHLGAGAKANHLTYIGDADIGERTNIGAGTVTCNYDGFFKHRTTIGKDAFIGSDTMLVAPVTVGDGAMTGSGSVVTKNVAPGDLAIGRARGVPAREVGRELERRSAG",
    "response#2": "MTVVILAAGKGTRMRSSLPKVLQPLAGQPLLQHVVNTAQCLQAEQILTVVGHQSHLVEEAMAEQGIYFVAQTEQLGTGHAVQQVVPEIDDMDTVLVLYGDVPLTAQTTLEDLLALVSEQHPLALLTAHLANPKGYGRIVRGSDGQVQAIVEEKDASDAQRAIDEINTGIMAAPTARLHQWLGALGNNNAQGEYYLTDIVAMAAAEGVPVVSAQPAAAWETLGVNSKVQLAQLERIHQQNQAHALLQAGVIIDDPDRTQVGSDVIVEPGAILRPGVYLRGRSRVSAGAQVGPDVTCIDSSIGRNSLVWYSVLRGATVGADCEVGPYASLRPEAVLEDGSKAGTFVEIKKSTIGPGAKVPHLSYIGDATIGADANIGAGTITCNYDGIAKHKTEIGKNAFIGSDTMLVAPVKIGDGAVTGAGSVITEDVEAEALSLERSPQISIANWARPVKKK",
    "response#3": "VILAAGKGTRMRSALPKVLHPLAGRPLIRHVIDAAHALGAVQICCVYGHGGELVPTTLAAAGCIWVEQTERLGTGHAVLQAMPALAAMNRVLILYGDVPLIEPETLNRLVADSAHQRLALLSITLPDPTGYGRICRDANGAVTGIVEHKDASPAQRAICEVYTGIMAAPTALLKHWLARLDNTNAQGEYYLTDVVAMAVAEQVPVVTAQPADEWEVLGVNSKQQLAELERIHQLNIARSLMAQGVTIVDPSATWIDAGVEVGEDSTLLPGVHLLGATAVGRDCRIGPNAVLRDTRLGERCIIGGSTIEGATLADDVQVGPYCHIRPGSTIEAGARLGNYAEVKASRIGAGSRVGHFSYVGDADVGEDVNIGAGTITANYDGAEKHRTVIGDGAFIGSDSMLVAPLSIGAGARTGVGSVVTRDVPPGASAIGVPARVKRPTRPEEE"
  },
  {
    "reference": "MLSSGDLTSASWELVVRVDHANGEQQTEITLRVSGDLHIGGVMLKLVEQMNIAQDWSDYALWWEQKRCWLLKTHWTLDKCGVQADANLLFTPQHKMLRLRLPNAKTVRLRVSFSAVVFKAVADICKVLNIRRPEELSLLKPSSDYCKKKKKKEKNSKEPVIEDILNLESSSTSSGSPVSPGLYSKTMTPTYDPINGTPALSTMTWFGDSPLTEQNCSVLAFSQPPPSPDVLADMFQPRSLVDKAKMNAGWLDSSRSLMEQSIQEDEQLQLRFKYYTFFDLNPKYDAVRINQLYEQARWAVLLEEIDCTEEEMLIFAALQYHISKLSQCAEIQDFATKSEVDEVEAALSSLEVTLEGGKADNTLEDITDIPKLADYLKLFRPKKLMLKACKQYWFVFKDTSIAYFKNKELEQGEPIEKLNLRGCEIVPDVNVSGRKFGIKLLIPVADGMNEVYLRCDHEDQYARWMAACILASKGKTMADSSYQPEVISILSFLKMKNRNSSPLVASSLENMDMNPECLVSPCCAKKHKSKQLAARILEAHHNVAQMPLVEAKLQFIQAWQSLPEFGLTYYLVRFKGSKKDDILGVAYNRLIRIDAVTGIPVTTWRFANMKQWNVNWEIRQVAIEFDQNVSIAFTCLSADCKIVHEYIGGYIFLSTRSKDQNETLDEDLFHKLTGGQD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that contributes to cell-matrix adhesion, negative regulation of gene expression, negative regulation of timing of anagen, positive regulation of integrin activation, negative regulation of stem cell proliferation by functioning in cytoplasm, cell-substrate junction, cytosol with integrin binding.2. The protein design should prioritize the formation of strand-strand-strand-helix-strand-turn-strand-helix-strand-strand-strand-strand-helix-strand-strand elements.3. The designed protein have FERM domains with specific sequence motifs that are crucial for its activity.4. The protein must exhibit the following characteristics: Involved in cell adhesion; Required for normal polarization of basal keratinocytes in skin, and for normal cell shape; When coexpressed with talin, potentiates activation of ITGA2B; Contributes to integrin activation; Required for normal keratinocyte proliferation. The designed protein sequence is ",
    "response#1": "MTAFDADDPATDNALLRYNIRQQTPDKPSPNMFYIDPEKGDIVTVVSPALLDRETLENPKYELIIEAQDMAGLDVGLTGTATATIMIDDKNDHSAKYELIIEAQDMAYWYWGRLSRGDAVSLLQGQRHGTFLVRDSGSIPGDFVLSVSESSRVSHYIVNSLGPAGGRRAGGEGPGAPGLNPTRFRIGDQEFDSLPSLLEFYKIHYLDTTTLIEPVAEVCDDAKNLSVVILGASDKDLHPNTDPFKFEIHKQAVPDKVWKISKINNTHALVSLLQNLNKANYNLPIMVTDSGKPPMTNITDLRVQVDVKVLTCEHCISQHPSCTWCTMPGFDENNRHSRCDTSERLLYNGCHDEFIENPKTEYYIARDEPLSNAGETEEESEAIQLKPQEILISMRPKDVVSFDVTFRQALDYPVDLYYLMDLSFSMADDKQKLTELGDLLSERMRKITKNFRLGFGSYVDKKVMPFVDARPERASKPCETCVDPYGFKNHMTLTKDTRRFAKEVNESAVSGNLDAPEGGFDAIMQALTCTDKIGWREKSRKMIVFSTDAGFHYAGDGRLGGLVVPNDGNCHLDNDGYYSVSTEFDYPSISQVYQKIRETQANMIFAVTEEQVPLYQRLRGALPEISASVGKLANDSSNVVELVQEQYDVCKKMNIINNNFQPRNHHHRSVMCCRKFHKKLSWSTTSTLRRVFKFPTLQHAKGRKVMNRTNVCDGIKVGDTITFNLTLEVTQCTDQREFLIKIGPSGLSEVLLIRLNVLCD",
    "response#2": "SCTECIRVDKDCSFCTDESFEEPRCDLRENLLRSGCGEASIVYTQGEMRTLKNSSINVSLQRTQVSPQAMYMRLRAGEEMSFNMDVFQPKESPVDLYILMDFSYSMSDDLDNLKSMGHNLADFLQALTSNYTIGFGKFVDKVSSPQTDMRPEKLREPWHNADSPFSFKNVIRLTSNINHFSQELSKERISGNLDAPEGGFDAILQTAVCKDKIGWRKDSTHLLVFSTESAFHYEADGTNVLAGILARNDEQCHLDSHGTYVYDTKQDYPSVPTLVRLLGQHNIIPIFAVTNHSYSYYEKLHKYFPISEIGELQEDSSNIVELLRVAFERIRSKMDIRADFTPRALKTEFTSPEFEKTESGSFNITRGKVSKFHMHVKALEYVGGQHVCSLPEKDRQGVIHVKPTSLSDSLTVSTAVICDVLPSFTETSYVTEVELQAELSYLNKAKWLEMYGRDGNDYSLGLTPTGVLVFEGETKIGLFFWPKITRLDFKKNKLTLVVVEDDEQGKEQEHTFVFRLEHPKACKHLWKCAVEHHAFFRLRGPVEAGWWKGELL",
    "response#3": "ITCRVSLLDGTDVSVDLPKKAKGQELFDQIMYHLDLIESDYFGLRFMDSAQVAHWLDGTKSIKKQVKIGSPYCLHLRVKFYSSEPNNLREELTRYLFVLQLKQDILSGKLDCPFDTAVQLAAYNLQAELGDYDLAEHSPELVSEFRFVPIQTEEMELAIFEKWKEYRGQTPAQAETNYLNKAKWLEMYGVDMHVVKARDGNDYSLGLTPTGVLVFEGDTKIGLFFWPKITRLDFKKNKLTLVVVEDDDQGKEQEHTFVFRLDHPKACKHLWKCAVEHHAFFRLRRDGNDYSLGLTPTGVLVFEGDTKIGLFFWPKITRLDFKKNKLTLVVVEDDDQGKEQEHTFVFRLDHPKACKHLWKCAVEHHAFFRLRGPVEAGWWKGELSRPVISITKSITVQPDKLDLKKKNPEDPSEIWMDVKACFQYTANPRNLNPRIKINYTFEAENERRQLGLPSRVRFKDYLSDQFTASTTLIGQNSKRCVTAKLVLQEKIKDKLRPIPIAVSVNIAGLESGSSSTRKERALPDLIPILNSNESETKITKVEFLKRDGNDYSLGLTPTGVLVFEGETKIGLFFWPKITRLDFKKNKLTLVVVEDDEQGKEQEHTFVFRLEHPKACKHLWKCAVEHHAFFRLRGPVEAGWWKGELVQCGEDNECHSNLKLQYRFCTREGNEDRFTYLPIENGIPVLVLKDQKDIALEITVTNNPSDARNPQKDGEDAYEAKLIATFPDSLTYSAFREMRGYPEKQLTCGANQNGSQAECELGNPFKRNSNVTFYLILSTTKVNVDTTDLD"
  },
  {
    "reference": "MDNDILKRLDPEVFEAIANETKRQTETLELIASENFTSKAVMEACGSVMTNKYAEGYPGKRYYGGCEFVDVAENLARDRAKKLFGCEYVNVQPHSGSSANMAVLFAVLKPGDAIMGLDLSHGGHLTHGSKVNFSGQFFDAHSYGVDKETGIIDMNKVEEMARRVKPKLIITGASAYSQGFDFKAFREVADKVGALLMADIAHPAGLVAAGLSANPMPHCHFVTTTTHKTLRGPRGGMIMMGKDFENPLGLTINTKNGSRVKMMSEVIDAEVMPGIQGGPLMHIIAGKAVAFGEALQPEFKAYAQQIKDNAAAMAAKFLAAGYHIVSGGTKNHLMLLDLRNKNVNGKVAENLLHEAGITVNKNMVPFDDKSPFVTSGIRIGTPAMTTRGMKVAEAEKIVEFIDRVISAANDANVADVCKAVRAEVRELCLGFPLNNYGSLV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a low Km value for (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O =   (6S)-5,6,7,8-tetrahydrofolate + L-serine.2. A protein that can modulate the overall flux of metabolites through the glycine biosynthesis pathway, enhancing or suppressing its activity as needed.3. The designed protein should be involved in glycine biosynthetic process from serine, tetrahydrofolate interconversion by localizing to cytoplasm and exhibiting glycine hydroxymethyltransferase activity.4. The protein should have a high affinity for (6S)-5,6,7,8-tetrahydrofolate.5. The protein design should incorporate a binding pocket that specifically recognizes pyridoxal 5'-phosphate to enhance enzymatic efficiency.6. The protein must exhibit the following characteristics: This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. The designed protein sequence is ",
    "response#1": "MKTDPEIAEAIRLETRRQAGKLELIASENFVSEAVLEAQGCIMTNKYAEGYPGKRYYGGCEYVDIAENLAIERCKALFGADYVNVQPHSGTQANMAVYFSALSVGDTILGMNLAHGGHLSHGSPANFSGKFYNVVPYGVDRETETIDYNQVEDLALQHKPRMIVVGASAYPRTIDFEKFRAIADKVGALVMADIAHIAGLVATGLHPSPVPVCEYVTSTTHKTLRGPRGGLVMCQASYQKTLSSRVFPGVQGGPLMHIIAAKAVAFKEALTDEFKDYQSQIVKNAQALAKELIGRGYRLVSGGTDNHLLLMDLTDKGLTGKEAQESLDSAGITVNKNGIPFDTRGPMVTSGIRIGTPALTSRGMKEEEMRTIARLVAHALHGDASPKDVRESVVRAVEKHHEWLKKCLPMIASENVTSPAVREMLVTDFGHRYAEGKPGERLYEGCEYIDEVELACVRLAKELFGAEHANVQPTSGVVANLAALFALTEPGDTILGLRISHGGHISHHDISAPGVRGLNVEYLPFDEEDMAIDVDGMVRKIEEVEPSVVMLGASLFLFPHPVEEAVEAVEAVGGYVVYDAAHVLGLIAGGQFQDPIREGAHVVTGSTHKTFPGPQGGIVLCQRDLADDIDEAVFPGLVSNHHLHHVAALAVALAEFKEYGERYARDTVRNAKALAEALYAEGLRVLCEHRGFTESHQIAVDVREQGGGAVIAEKLESANILCNKNLLPWDDESKSHDPSGIRLGTQELTRLGMGLSEMEYIAELLSERDPELFGAITDELGRQRDEIELIASENIVSAAVMEAQGSVLTNKYAEGYPGRRYYGGCQYVDVAENLAIDRAKQLFNCEFANVQPNSGSQANQGVFQAILKPGDTILGMDLASGGHLTHGAAPNQSGKWFNAVHYGVRESDCLIDYDQVQALATEHQPKLIIAGGSAIPRQIDFAKFREIADSVGAYLLVDMAHFAGLVAAGEHPSPFPHADVATTTTHKTLRGPRGGMILTNNPDLAKKFNSAIFPGIQGGPLMHVIAGKAAAFGEALKPEFKSYQKQVRANAVALADELIKGGLDIVTGGTDTHVMLVDLRPKGVTGNIVDKALGRAHITTNKNGIPFDPEKPTVTSGIRLGTPAGTTRGFGEEEFREIARL",
    "response#2": "MKTDPEIAEAIRLETRRQAGKLELIASENFVSEAVLEAQGCIMTNKYAEGYPGKRYYGGCEYVDIAENLAIERCKALFGADYVNVQPHSGTQANMAVYFSALSVGDTILGMNLAHGGHLSHGSPANFSGKFYNVVPYGVDRETETIDYNQVEDLALQHKPRMIVVGASAYPRTIDFEKFRAIADKVGALVMADIAHIAGLVATGLHPSPVPVCEYVTSTTHKTLRGPRGGLVMCQASYQKTLSSRVFPGVQGGPLMHIIAAKAVAFKEALTDEFKDYQSQIVKNAQALAKELIGRGYRLVSGGTDNHLLLMDLTDKGLTGKEAQESLDSAGITVNKNGIPFDTRGPMVTSGIRIGTPALTSRGMKEEEMRTIARLIADKLHTTTDPHLVATVAADHAVAAAIADEETRQRDSIELIASENFVSRAVLDAQGSVLTNKYAEGYPHRRYYGGCANVDAIEDLVIARVNQLFGSAYANVQPHSGSQANQAVFLALLAPGDTILGLDLKAGGHLTHGAPVNMSGRWFKAVSYGVDPETHRIDMDQVAVQARQHRPRLLIAGGSAYPRIIDFGRFRQIADEVGAILMVDMAHFAGLVAGGVYPSPVPFADVVTSTTHKTLRGPRGGFVLTNDANIAKKINSATFPGLQGGPLMHVIAAKAVAFGEALQPEFGAYAQAVVENCRVLAQALADGGLTITSGGTDCHLAVVDLRPFGVTGNIAEQALESVGITLNKNAIPNDPEKPMVTSGIRVGTAAGTSRGFGADQYREIAGKLMAGILNG",
    "response#3": "MKTDPEIAEAIRLETRRQAGKLELIASENFVSEAVLEAQGCIMTNKYAEGYPGKRYYGGCEYVDIAENLAIERCKALFGADYVNVQPHSGTQANMAVYFSALSVGDTILGMNLAHGGHLSHGSPANFSGKFYNVVPYGVDRETETIDYNQVEDLALQHKPRMIVVGASAYPRTIDFEKFRAIADKVGALVMADIAHIAGLVATGLHPSPVPVCEYVTSTTHKTLRGPRGGLVMCQASYQKTLSSRVFPGVQGGPLMHIIAAKAVAFKEALTDEFKDYQSQIVKNAQALAKELIGRGYRLVSGGTDNHLLLMDLTDKGLTGKEAQESLDSAGITVNKNGIPFDTRGPMVTSGIRIGTPALTSRGMKEEEMRTIARLLAQTDPELAKAIELEHQRQQRNIELIASENFVSPAVLEAQGSILTNKYAEGYPGKRYYGGCEFVDIAESLAISRAKKLFGADHANVQPHSGAQANFAVYFALLQPGDKILGMNLAHGGHLTHGSPVNVSGKYFNVVAYGVEEDTGCINYEKLREIALQEKPKMIVAGASAYARAIDFKKIGEIAKEIDAYFFVDMAHIAGLVAAGLHQSPVPYADVVTTTTHKTLRGPRGGMILCKEEYAQLIDKAIFPGSQGGPLMHVIAAKAAAFGEALKPEFKAYQQQIINNAQALAKGLLERGFNLVSGGTDNHLILVDLRGTGITGKQAETLLDEVHITCNKNAIPFDPEKPFVTSGIRLGTPAVTTRGFKEKDMDKVAEIIARALAGQAGTVAADHAVAAAIADEETRQRDSIELIASENFVSRAVLDAQGSVLTNKYAEGYPHRRYYGGCANVDAIEDLVIARVNQLFGSAYANVQPHSGSQANQAVFLALLAPGDTILGLDLKAGGHLTHGAPVNMSGRWFKAVSYGVDPETHRIDMDQVAVQARQHRPRLLIAGGSAYPRIIDFGRFRQIADEVGAILMVDMAHFAGLVAGGVYPSPVPFADVVTSTTHKTLRGPRGGFVLTNDANIAKKINSATFPGLQGGPLMHVIAAKAVAFGEALQPEFGAYAQAVVENCRVLAQALADGGLTITSGGTDCHLAVVDLRPFGVTGNIAEQALESVGITLNKNAIPNDPEKPMVTSGIRVGTAAGTSRGFGADQYREIAG"
  },
  {
    "reference": "MSGTLHNTVGSGILPYQQEIRIKLTSNEPIKDSEWSITGYPNTLTLQNAVGRTNNATEKNLALVGHIDPGNYFITVKFGDKVEQFEIRSKPTPPRIITTANELRGNSNHKPEIRVTDIPNDTTAKIKLVMGGTDGDHDPEINPYTVPENYTVVAEAYHDNDPSKNGVLTFRSSDYLKDLPLSGELKAIVYYNQYVQSNFSNSVPFSSDTTPPTINEPAGLVHKYYRGDHVEITLPVTDNTGGSGLRDVNVNLPQGWTKTFTINPNNNTEGTLKLIGNIPSNEAYNTTYHFNITATDNSGNTTNPAKTFILNVGKLADDLNPVGLSRDQLQLVTDPSSLSNSEREEVKRKISEANANIRSYLLQNNPILAGVNGDVTFYYRDGSVDVIDAENVITYEPERKSIFSENGNTNKKEAVITIARGQNYTIGPNLRKYFSLSNGSDLPNRDFTSISAIGSLPSSSEISRLNVGNYNYRVNAKNAYHKTQQELNLKLKIVEVNAPTGNNRVYRVSTYNLTNDEINKIKQAFKAANSGLNLNDNDITVSNNFDHRNVSSVTVTIRKGDLIKEFSSNLNNMNFLRWVNIRDDYTISWTSSKIQGRNTDGGLEWSPDHKSLIYKYDATLGRQINTNDVLTLLQATAKNSNLRSNINSNEKQLAERGSNGYSKSIIRDDGEKSYLLNSNPIQVLDLVEPDNGYGGRQVSHSNVIYNEKNSSIVNGQVPEANGASAFNIDKVVKANAANNGIMGVIYKAQLYLAPYSPKGYIEKLGQNL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be expressed specifically in the plasma membrane2. The engineered protein is expected to adopt a helix-helix-helix-helix-helix-helix-helix-strand-helix-helix-helix-helix-helix-helix-based conformation.3. Target a Polar residues, Basic and acidic residues, Basic residues compositional bias in the protein's composition for improved properties.4. The protein contains novel FIVAR domains that confer a unique function or activity.5. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "TIPFYLILLLLPLLNFRSASFFARFTFLGTISVIYLIFLVTYKAIQLGFHLEFHWFDSSMFFVPEFRTLFPQLSGVLTLAFFIHNCIITLMKNNKHQENNVRDLSLAYLLVGLTYLYVGVLIFAAFPSPPLSKECIEPNFLDNFPSSDILVFVARTFLLFQMTTVYPLLGYLVRVQLMGQIFGNHYPGFLHVFVLNVFVVGAGVLMARFYPNIGSIIRYSGALCGLALVFVLPSLIHMVSLKRRGELRWTSTLFHGFLILLF",
    "response#2": "VFGILGNIISFVVFLAPVPTFVRICKKKSTEGFQSLPYVSALFSAMLWIYYAMQKDGTAFLLITINAFGCVIETIYIVLFVSYANKG",
    "response#3": "TIPFYLILLLLPLLNFRSASFFARFTFLGTISVIYLIFLVTYKAIQLGFHLEFHWFDSSMFFVPEFRTLFPQLSGVLTLAFFIHNCIITLMKNNKHQENNVRDLSLAYLLVGLTYLYVGVLIFAAFPSPPLSKECIEPNFLDNFPSSDILVFVARTFLLFQMTTVYPLLGYLVRVQLMGQIFGNHYPGFLHVFVLNVFVVGAGVLMARFYPNIGSIIRYSGALCGLALVFVLPSLIHMVSLKRRGELRWTSTLFHGFLILLL"
  },
  {
    "reference": "MSITIRTLQKRKQDGGKFSVLTAYDACFSRLISEAGVEAILVGDSLGNVIQGQTSTVPVTLEQMVYHTQCVARGNQGSLIIADIPFMGAATLERTLNAATALMQAGANMVKLEGSAWLAEAIEVLNRNGVPVCAHMGLTPQAVNSLGGYRVQGKDDDGAAELLNAAKILDQAGTALFVLECVPRALSAEITREVAAPVIGIGAAPECDGQVLVMHDMLGISPGKPARFVRNFMAETGDMRAAFKAYDDAVKDGSFPADEHCF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have 3-methyl-2-oxobutanoate hydroxymethyltransferase activity within cytoplasm to regulate pantothenate biosynthetic process.2. The protein must exhibit the following characteristics: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha- ketoisovalerate to form ketopantoate..3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.4. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-   oxobutanoate + H2O = (6S)-5,6,7,8-tetrahydrofolate + 2-   dehydropantoate are properly positioned for optimal catalysis.5. The designed protein should have a high specificity for 3-methyl-2-oxobutanoate binding, with minimal off-target effects.6. A protein that is capable of catalyzing reactions within the (R)- pantoate from 3-methyl-2-oxobutanoate: step 1/2 pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MSVSGTVDRAGGGPGARVVDVPGRRGGRRPAPLREVAAAAGVDLTTPVADDVVASLDAVVDEVVVFGERPTAERRQALLDEGLPAFLVDQSGRAGQLLARTAVVFFGDPTVSLAAVAGTRVATVAVYGRPELAGRRTAVLATDLPRVLGQLVPLLFGDDPASRVMA",
    "response#2": "MSDALASALDWLRAHAEHATVGLSGGVDSAVVAALAVRALGDSRVRAVTVRMPDGGAAPPGYADAQAAADAVGIAHETVEFSALRGDMLAGLTGADEDEAAARARANMRMRVLYAWAADRLGADALVTGVCETDFSGYPDCRDAFLRALAPLAHLAKTAVFRAARARDGLTAEALAAAIGAPEADLVKQHPFPGPGLGVRILADVRADYCETLRRADAIFIAELETPWTAPGHPQGLPAWLPVHTLPLGVEGVGVMGDQRVYAYPVLLRASGAYGDFWLRAAAMAAPDLPSISRMTPVAVEWE",
    "response#3": "MFNDLLAXIXGXLTGXGEVXVXDSTRDXDAXLGFLALEGXQHDGHDFVEAXLXKGAAALVTXGXRVDXGXGXAXVXVADSRKALAXLSAXFYAGQLXAXMAXVAXIVXPTAXVXDVAIGAXAVIEXGVXVGXGVXIGAXIAXVXLGAVXHXLGNXII"
  },
  {
    "reference": "MTSMERMERDAMETVAPYARVTYHRRVRGDLDDTLPKPYLPRALQAPDMEHPQGTPDHRHNGLSVLQQHVAFFDLDNNGIIYPFETFSGFRLLGFNLLASLILAAGINIALSYATLPGWLPSPFFPIYIHNIHKAKHGSDSKTYDNEGRYTPANLELMFSKYARTIPDKLSLGELWDMTEGNRDAFDFFGWLASKVEWGVLYALASDEEGFLSKEAIRRCFDGSLFEYCAKNYAEIKEYKTYY",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Can catalyze sulfoxidation of thiobenzamide, hydroxylation of aniline and epoxidation of oleic acid.2. The designed protein must be optimized for plant seed peroxidase activity. The designed protein sequence is ",
    "response#1": "AFARAWFKLTHRDMGPVVRYLGPEVPNETLLWQDPLPEATYEAIDGADVAALKEQIKATGLTVSQLVSTAWASASTFRSGDKRGGANGSRIRLEPQSGWEVNNPAELATVLRTLEGVQQDFNAGLDGGKQVSLADVIVLAGGVGVELGAQAAGYAIEVPFTGGRVDAAQDETDVESFSYLEPKADGFRNFLGKGNRIPGEFLLVDRANLLGVSAPEMTALVGGLRVLGANYDGSSLGVLTDNPGSLTNDFFVNLLELGTTWTATDETSTTFQSRDASGEVRWTGSRADLVFGSNSELRALAEVYASR",
    "response#2": "AFARAWFKLTHRDMGPVVRYLGPEVPNETLLWQDPLPEATYEAIDGADVAALKEQIKATGLTVSQLVSTAWASASTFRSGDKRGGANGSRIRLEPQSGWEVNNPAELATVLRTLEGVQQDFNAGLDGGKQVSLADVIVLAGGVGVELGAQAAGYAIEVPFTGGRVDAAQDETDVESFSYLEPKADGFRNFLGKGNRIPGEFLLVDRANLLGVSAPEMTALVGGLRVLGANYDGSSLGVLTDNPGSLTNDFFVNLLELGTTWTATDETSTTFQSRDASGEVRWTGSRADLVFGSNSELRALAEVYASR",
    "response#3": "VSWADLIVLAGNVALESMGFETYGFAFGREDVWEPEEVIWGPEDTWLGDERYVGEKEMAEDVGATEMGLIYVNPEGPAGNPDPLAAAHFIRETFKRMAMDDEETVALIAGGHTFGKTHGAGPADAHVGPEPEGAPVENQGLGWLSSYGSGKGPDTITSGLEVTWTDVPTQWSNRFFEILFAHEWELTKSPGGAHQWVAKDGEAIIPDAFDPEKKHRPTMLTTDLSLRVDPVYEQISRRFLENPEEFRLAFAKAWYKLLHRDMGPVGPHMLS"
  },
  {
    "reference": "MSPRQLLDQLEAMGVVPTGVADDSRQVLPGDIFLAYPGDLADGRRYIADALTRGAVAVLWQPGGDFNFSFQVANLSVESLRPLAGPLAHAVYGHPSEGLSLIAVTGTNGKTTISQCLAKAYPKPCAIIGTLGAGFPDGLTETGFTTPEATTLMRYLAQFRTAGAAACALEASSIGIEEGRMNGARVDVAVFTNMTRDHLDYHGTMEAYAEAKKKLFLWPRLRTAVINLDDELGRILSRETTAQRILGYAIGEQRRDYPALVRAENLVDTPFGQRFTLVVPNGRAVVDSGLVGCYNISNLLAVAAVLHDAGVQAVEVARRLSELMPPPGRMERVGGQGEPLIVVDYAHTPDALENALGALRGVANSRGGRLCVVFGCGGDRDPGKRPQMGEVAERLADRVLVTSDNPRSEAPQAIIAAIVAGMSQAEVEVDRAAAILRVIGEADAADVVLLAGKGHESYQEVAGVRHHFSDVEQAGAALAMRNPENKEVAA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a high specificity for ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding, with minimal off-target effects.2. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.3. The designed protein should have magnesium ion binding, ATP binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity within cytoplasm to regulate cell division, regulation of cell shape, cell cycle, peptidoglycan biosynthetic process, cell wall organization.4. The presence of Mur ligase C-terminal, Mur ligase N-terminal catalytic, Mur ligase central domains is critical for the interaction of the designed protein with its intended targets.5. The desired protein should be able to perform ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate6. A protein that can modulate the overall flux of metabolites through the peptidoglycan biosynthesis, Cell wall biogenesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MKLKDILYKVKITAVKGTTEVAVTGFAIDNRKVAPGTVFGAFRGSEANGEDFIPAAIAAGAIAIVARPGVEVPDIDAITFDSRSVKSSTLFVAIKGTEVDGHNYIQKAIDLGASVIVCEILPIDLNQEVTYIQVENSNEALAFLASNFYGNPSQKLKLVGTIVTGTNGKTSVTHWITQAFEYLDRKAAVVGTLGNGFLGKLESTQNTTPGSIELQKMLAGYVNEGADVVAMEVSSHGLDQGRLSGTQFDVAVLTNLTRDHLDYHRTMAAYQEAKRRLFAWGTLKASVINHDDAFGQELADELHLMQQHTITYGIHSGEVRATAVELHQDHFTMHVISPYGNADVRVNLIGEFNVYNVLAVLSGLAIGFDMDTAIASIESFSGVPGRFEIVSSNQDGPIVIVDYSHTPAGIENVLQAVRNIAPEVKLTIVFGCGGDRDRSKRPQMASSAEVHADQVIITTDNPRNEDPRKIIDDAMAGFEYPESVIVEEDRESAIRIAIQSSKSGEVVVIAGKGHETYQILDDVIVPFDDRKVAKQALESL",
    "response#2": "MRQLATDSRAVTPGDTFVAYPGNRADGRQLINQAIKNGANAVIWEAQGFNWNESWNIPNRAVANLRQQAGEIANHVVTGICTNSLACQPGDLFIGMPGTRVDGGEFWAGALAAGAVAALVSPAIAQSHAAKIGDACVISVDSIVDACAQLAAAFYGHPSKHMTLIGITGTKGKTTTTYLVKSILENAGHKVGLLGTIEVIIGDEHIPAERTTPESRAIQEYFARMREAGCDTVVMEVSSQGLMLHRTQGFVFDYGIFTNLTQDHIGEGEHASFEEYLACKGLLFRQCRIGIVNRDDEHYDRVIEGHTCALETYGFSDEADLRASNPVLEPGSGLLGVRYHVEGLLDFDVEIDVPGRFSVYNSLTAIAICRHFGVSEEDMRRALRDVRVRAGRFEIVSSNQDGPIVIVDYSHTPAGIENVLQAVRNIAPEVKLTIVFGCGGDRDRSKRPQMASSAEVHADQVIITTDNPRNEDPRKIIDDAMAGFEYPESVIVEEDRESAIRIAIQSSKSGEVVVIAGKGHETYQILKDKTIHMDEREIVAEIIRGIRE",
    "response#3": "MTLDEITGLTLDNRQVQPGMAFVALRGTRQHGLDYAEAAVRAGASVVLYEPEIGLQPPVLSVDAVSVPALREHLGTLADRLFANPARRLRVIAVTGTNGKTSVVHFLSQLWGWAGYPWATLGTIGALSSRPDFHFQTASLTTPDVVSTARFFHQADECGIQYAAVEASSHGLDQKRLGKGPFEVGIWTNFSQDHLDYHKTFQAYWQAKSRLFAIVAKYAIVHASVPDQDQLFEICRKKKVAVSLYGNPSCPAFYQPLKIQPDGAVIHIQIESFQWQGFIPLMGLFQYDNLVAALYTLLALGMPLAEALPRLASSAAVPGRVQRIERGQEFLAVVDYAHKPAALEAVIATLRGQSAGRLAVVVGAGGDRDTGKRPQMGEVAARSADLLVVTDDNPRSENPAEIRREVMAGALAVPVTERGEVREIADRAHAIADAVRWARSGDVVLVAGKGHEPYQEVAGVRHPFDDREQARRALAQRRATTPS"
  },
  {
    "reference": "MPLGAPALLALALGLGLWLGALAGDPGRGCGPCPLPCFCGPAPDAACRVNCSGRWLQTLGPSLRIPADATALDLSHNLLQTLDIGLLVNLSALVELDLSNNRISTLEEGVFANLFNLSEINLSGNPFECNCGLAWLPRWAKEHQVHVVQSEATTCRGPIPLAGQPLLSIPLLDNACGEEYVACLPDNSSGAVAAVPFYFAHEGPLETEACSAFCFSAGEGLAALSEQNQCLCGAGQASNSSAACSSWCSSISLSLNSACGGPTLLQHTFPASPGATLVGPHGPLASGQPADFHITSSLPISSTRWNFGDGSPEVDMASPAATHFYVLPGSYHMTVVLALGAGSALLETEVQVEATPTVLELVCPSFVHSNESLELGIRHRGGSALEVTYSILALDKEPAQVVHPLCPLDTEIFPGNGHCYRLVAEKAPWLQAQEQCRTWAGAALAMVDSPAIQHFLVSKVTRSLDVWIGFSSVEGTEGLDPRGEAFSLESCQNWLPGEPHPATAEHCVRLGPAGQCNTDLCSAPHSYVCELRPGGPVWDTENFVMGMSGGGLSGPLHPLAQQETVQGPLRPVEVMVFPGLSPSREAFLTAAEFSTQKLEEPAQMRLQVYRPSGGAAAVPEGSSEPDNRTEPAPKCVPEELWCPGANVCIPFDASCNSHVCINGSVSRLGLSRASYTLWKEFFFSVPAGPPTQYLVTLHSQDVPMLPGDLIGLQHDAGPGTLLQCPLASSCPGQALYLSTNASDWMTNLPVHLEEAWAGPVCSLQLLLV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Component of a heteromeric calcium-permeable ion channel formed by PKD1 and PKD2 that is activated by interaction between PKD1 and a Wnt family member, such as WNT3A and WNT9B; May be an ion-channel regulator; The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling; The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow- induced signaling; Acts as a regulator of cilium length, together with PKD2; Involved in renal tubulogenesis.2. The protein should exhibit Polar residues compositional bias.3. A protein that can perform transmembrane transporter binding, carbohydrate binding, cation channel activity in cation channel complex, membrane, endoplasmic reticulum to support spinal cord development, positive regulation of cyclin-dependent protein serine/threonine kinase activity, positive regulation of cytosolic calcium ion concentration.4. The protein contains novel LDL-receptor class A; atypical, LRRNT, GPS domains that confer a unique function or activity.5. Design the protein with a signal peptide incorporated.6. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "ELNCNVSRELHYSWTVYDSAGLTFPLPLTNTHRQSLVLQSYTLPYGIYKAIARVQIVGSVVYSNYSVRLQVVPNSVVVVIQGGTNIYVNTKSSNEVTLDGQASYDPDFPLNPLRYSWTCQPVSTISSSCFSQSIPTSYPVLKFPTSLLKSNFDQFKFTLTVHSGERSASSEIFLTLTSHLAGKLFVHCPECQGDQVSWDQSFSVRAECEDCNVSAEFIQYSWSPGTNLTFLWNFGDGTVRTGLSTEQHVFTRTGEFRVMVIASNLVSSASLSSYLFLPVCQPPPVKNMGPAKVQIWRSQPLRLGVTFEAAILCNISQGLSYTWSFVSAEMTTVTLPTAVNTRRQTIMLPSYTLECGNYTAIAKVQIKGSMVYSNYCVGVEVRARAPVSVISEGTHIFISTATSTFIILRGAQSYDPDNPGAALRYHWTCTAASSPRWPCFDNSTSYQVDTQAPAISFPAKWLSECCDQFLVTLTVSSRGQNSSQALMFLSTRPDLAFRFVHISWVNFRDISVNWNEEVSLRAVCEDCGDVPDLTYSWDLFLVNATEKSAVEVPFCSTVGLLGALALGTSLKSSKSDLPSNLRAPLTPHSPSPGATLVGPRGPLASGQPAAFHIAAPLPVSSARWDFGDGSPRVDVTGPAATHRYVLPGHYHVTAVLALGAGLVPLETEVQVSALNRLVHEPSVLQNHTVTVSCRVNVGTNLTFLWNFGDGTVRTGLSTEQHVFTRTGEFRVMVIASNLVSSASLSSYLFVVD",
    "response#2": "ELNCNVSRELHYSWTVYDSAGLTFPLPLTNTHRQSLVLQSYTLPYGIYKAIARVQIVGSVVYSNYSVRLQVVPNSVVVVIQGGTNIYVNTKSSNEVTLDGQASYDPDFPLNPLRYSWTCQPVSTISSSCFSQSIPTSYPVLKFPTSLLKSNFDQFKFTLTVHSGERSASSEIFLTLTSHLAGKLFVHCPECQGDQVSWDQSFSVRAECEDCNVSAEFIQYSWSLNRLVHEPSVLQNHTVTVSCRVNVGTNLTFLWNFGDGTVRTGLSTEQHVFTRTGEFRVMVIASNLVSSASLSSYLFVVDPASPGATLVGPRGPLASGQPAAFHIAAPLPVSSARWDFGDGSPRVDVTGPAATHRYVLPGHYHVTAVLALGAGLVPLETEVQVETHTLPVSIITPELNCNVSRELHYSWTVYDSAGLTFPLPLTNTHRQSLVLQSYTLPYGIYKAIARVQIVGSVVYSNYSVRLQVVPNSVVVVIQGGTNIYVNTKSSNEVTLDGQASYDPDFPLNPLRYSWTCQPVSTISSSCFSQSIPTSYPVLKFPTSLLKSNFDQFKFTLTVHSGERSASSEIFLTLTSHLAGKLFVHCPECQGDQVSWDQSFSVRAECEDCNVSAEFIQYSWSEEGKIPLHWAANHKDPSAVHTVRCILDAAPTESLLYTELNCNVSRELHYSWTVYDSAGLTFPLPLTNTHRQSLVLQSYTLPYGIYKAIARVQIVGSVVYSNYSVRLQVVPNSVVVVIQGGTNIYVNTKSSNEVTLDGQASYDPDFPLNPLRYSWTCQPVSTISSSCFSQSIPTSYPVLKFPTSLLKSNFDQFKFTLTVHSGERSASSEIFLTLTSHLAGKLFVHCPECQGDQVSWDQSFSVRAECEDCNVSAEFIQYSWS",
    "response#3": "GEEYVACLPDNSSGTVVTAAFSVAHEGLLDPEACGAFCFSAGQGLAALSEQGQCLCGAAQPPNTSSACLSLCSSPLPPPAPAAACGGPTLLQHVFRCPCNLTAGACDVRCCCDQECSSNLTTLFRRSCFTGVFGGDVNPPFDQLCSAGTTTRGVPDWFPFLCVQSPLANTPFLGYFYHGAVSPKQDSSFEVYVDTDAKDFADFGYKQGDPIMTVKKAYFTIPQVSLAGQCMQNAPVAFLHNFDVKCVTNLELYQERDGIINAKIKNVALGGIVTPKVIYEEATDLDKFITNTETPLNNGSTPRIVNVEEHYIFKWNNNTISEINVKIFRAEINAHQKGIMTQRFVVKFLSYNSGNEEELSGNPGYQLGKELNCNVSRELHYSWTVYDSAGLTFPLPLTNTHRQSLVLQSYTLPYGIYKAIARVQIVGSVVYSNYSVRLQVVPNSVVVVIQGGTNIYVNTKSSNEVTLDGQASYDPDFPLNPLRYSWTCQPVSTISSSCFSQSIPTSYPVLKFPTSLLKSNFDQFKFTLTVHSGERSASSEIFLTLTSHLAGKLFVHCPECQGDQVSWDQSFSVRAECEDCNVSAEFIQYSWSSPGATLVGPRGPLASGQPAAFHIAAPLPVSSARWDFGDGSPRVDVTGPAATHRYVLPGHYHVTAVLALGAGLVPLETEVQVGTDVAYLWNFGDDTIELGSSSSSHVYSREGEFTVEVLAFNNVSSTTLRKQLFSVSVYTNGTVFAANTNITFVAITEETIPLEFAWYFGENPPVMTTSRSIRRRLSVPQWYRVKVKATSRIGSVVSEPHLIRVQK"
  },
  {
    "reference": "MAVYVVDHPLVRHKIGILRMESTSTSEFRSVSNEVARLLIYEATKGFRTEKHTVQGWAGPVEIEAISGKKVTVVPILRAGLGLMDGVLDMIPGAKISVVGLYRNEETLEPVEYYVKLASDMDQRLAIILDPMLATGGSLIATIELLKRHGCRQICSLNLVCAPEGIAKVEAAHPDVDIYTAAIDDHLNEQGYIIPGLGDAGDRIFGTK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.2. The protein should be designed to catalyze the following reaction diphosphate + UMP = 5-phospho-alpha-D-ribose 1-diphosphate +   uracil.3. A protein should be able to manipulate the activity of enzymes in the UMP from uracil: step 1/1, UMP biosynthesis via salvage pathway pathway, either by activating or inhibiting their function.4. The designed protein should have magnesium ion binding to facilitate uracil salvage.5. For general function, the protein need meet that Catalyzes the conversion of uracil and 5-phospho-alpha-D- ribose 1-diphosphate (PRPP) to UMP and diphosphate.6. The designed protein should be able to compete with endogenous proteins or inhibitors for 5-phospho-alpha-D-ribose 1-diphosphate, uracil binding. The designed protein sequence is ",
    "response#1": "VMDHPLIQHKISLLRDKNTQTKEFRELVSEISMLMGYEVTRNMPLKEVEIETPVGVARTKVISGKKMGIVPILRAGLGMVDGMLKLLPMAKVGHIGLYRDPETLQPVEYYCKLPCDIAEREIVVLDPMLATGGSASAAIKYLKERGVTSIKLMCLIASLDGIKKINDEHPDVLIYCAAVDDKLNEHGYIIPGLGDAGDRLFGSRYDVVGFLGDSPYLMETLTDLRKISTENISFRKGLVRLGRYMGYELTKTMEFEKMPIQTPLEKTEGVFAKDRKNVVIITILRAAFPLMEGLIKNFESAKVGIVSASRGHAPDFKIEMNYIKVPQLNPEDTVIVSDPMIATGSTLIHVLKEFRDSKPKRMMIVGVLAAPEGINAVKKEFSDVEIFVTKIDENLNKDGYIVPGLGDAGDRAFL",
    "response#2": "VMDHPLIQHKISLLRDKNTQTKEFRELVSEISMLMGYEVTRNMPLKEVEIETPVGVARTKVISGKKMGIVPILRAGLGMVDGMLKLLPMAKVGHIGLYRDPETLQPVEYYCKLPCDIAEREIVVLDPMLATGGSASAAIKYLKERGVTSIKLMCLIASLDGIKKINDEHPDVLIYCAAVDDKLNEHGYIIPGLGDAGDRLFGSKILL",
    "response#3": "VMDHPLIQHKISLLRDKNTQTKEFRELVSEISMLMGYEVTRNMPLKEVEIETPVGVARTKVISGKKMGIVPILRAGLGMVDGMLKLLPMAKVGHIGLYRDPETLQPVEYYCKLPCDIAEREIVVLDPMLATGGSASAAIKYLKERGVTSIKLMCLIASLDGIKKINDEHPDVLIYCAAVDDKLNEHGYIIPGLGDAGDRLFGSATVERGTHALAKDTLSRLRDVETEQVAFRKGLVKLGRICGYEIIDGAMETEYVPVQTPLQETTGERVKGLDDVVIINVLRAATPFVEGLLKAFPRAKQGVISAGRDEEAGMTDDGEFPITIDYVKLPEIRPEDTVIVADPMLATGSTMCAVLDHVLDEADAFEDLFVLSAVSAPEGLVRVSESVPEADLLTVAIDDRLDDDGFILPGLGDAGDRAFRL"
  },
  {
    "reference": "MDIQWFATGVGAAVVLYIFYHFIRIILNILVPYAFCQPIDLKKKAGASWAVVTGATDGIGKSYSFELARRGFNVYIVSRTQSKLEQTKKDILEKQPDIEVRFATYDFTNPSVTDYEKLLSKLNEVSVGILINNVGMFFDYPEMLHKINGGIDSIANVIIINTLPATLLSAGILPQMVSRKAGIIVNIGSFAGVVKLAEWSIYSATKKYVEWLTGCLRKEYSHHGIIFQAITPAMVATKMAGNPNTSFFCPDSDTFARSALNTIGHASETTGYIAHQIQCEILKLLPDFVIDRSIKKGNAEFREKALAKSENKPLA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit oxidoreductase activity. The designed protein sequence is ",
    "response#1": "PGIFTFEMFKPQFCEMLLAEVEHMEKWVYDSRSTIMRPNTMNNFGVVLDDFGFDSMLQKLVDDFISPIAQVLFPEVCGTSLDSHHGYIVEYGKDRDVDLGFHVDDSEVSLNVCLGKQFSGGELYFRGVRCDKHVNSDSTEKEVYDYSHVPGHAILHRGRHRHGARATTSGHRANLILWCG",
    "response#2": "PGIFTFEMFKPQFCEMLLAEVEHMEKWVYDSRSTIMRPNTMNNFGVVLDDFGFDSMLQKLVDDFISPIAQVLFPEVCGTSLDSHHGYIVEYGKDRDVDLGFHVDDSEVSLNVCLGKQFSGGELYFRGVRCDKHVNSDSTEKEVYDYSHVPGHAILHRGRHRHGARATTSGHRANLILWCR",
    "response#3": "PGIFTFEMFKPQFCEMLLAEVEHMEKWVYDSRSTIMRPNTMNNFGVVLDDFGFDSMLQKLVDDFISPIAQVLFPEVCGTSLDSHHGYIVEYGKDRDVDLGFHVDDSEVSLNVCLGKQFSGGELYFRGVRCDKHVNSDSTEKEVYDYSHVPGHAILHRGRHRHGARATTSGHRANLILWCG"
  },
  {
    "reference": "MLNEVGKINIKGINHLLFSVSNLEKSIEFYEKVFHAQLLVKGQKTAYFDLNGLWLALNLEADIPRNEIHKSYTHMAFTIDPKDFDAIHHRLKNLNVNILNGRPRDKQDQKSIYFTDPDGHKFEFHTGTLQDRLSYYKKDKPHMKFYI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a high affinity for Mg(2+).2. For general function, the protein need meet that L-cysteine is probably the physiological thiol donor.; Metallothiol transferase which confers resistance to fosfomycin by catalyzing the addition of a thiol cofactor to fosfomycin3. The protein requires Mg(2+) to activate a critical catalytic site.4. A protein that can perform transferase activity, transferring alkyl or aryl (other than methyl) groups, magnesium ion binding in cytoplasm to support response to antibiotic.5. The protein contains novel VOC domains that confer a unique function or activity.6. The Proton donor/acceptor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "ELSDADAVWQKRKAHALMLSGVTILKPETVYFSYDTEIEPDVVIEPNVYFGLGVKVHSGSVIHAFSYLTGAVVGANARIGPYARLRPGTELARSVKVGNFCEIKQAKVGEATKINHLSYIGNAEIGAASNIGAGTITCNYDGFHKYKTEIGDNAFIGSNSALIAPLIIGKSSYIASGSVITENVPINSIAFGRARLEAV",
    "response#2": "VVLAAGQGTRMKSKRPKVLHEIGGRPLVGHVLTQAAALDASYVEVVVRHERDQVVSALSAQYPGAVIVDQDEIPGTGRAVQVALDALPADFDGDVLVLSGDVPLLEADTLRALIDGHRAASAAATLLSAVLDDPTGYGRIIRQAGKVVAIVEQKDASAEQLSIQEVNTGVLALPTVALKRWLPQLSSDNAQGEYYLTDVIAMAVADGLQVVTAQPADAFEVEGVNNRLQLAALERAFQARQAEALMVNGVTLADAARIDVRGGLHCGADVFIDINCVFEGDVTLEDGVRIGANCVLRNSTVGARSNLKPFSLIEDAEIGTDCSIGPFARLRPGTTMGVGAKIGNFVETKKTVIGDGSKINHLSYVGDTDIGRNVNIGAGTITCNYDGANKHRTVIEDDAFIGSDTQLVAPVKVGRGATLGAGTTLTADAPAGELTISRARQRHITGWKRPKKA",
    "response#3": "TPKVLHAIAGRPLIYFSVRAALDAGCEEVVVVVGHGKQAVADYLEKTFGDQVKTAVQQEQRGSGDAAWIGIQAMTHAAHHVVLWYGDAPLVSAAHLLDVVACSRGLAGGPTLAMATCLHPEPFGYGRVIRNTQGNVICIREEKDLTTDRERAIQEVNVGIYVAPSSFFRKTLPQLKTNNAQRELYLTDIVALGLSHLPLPATRIGSATPKVLHELGGRSMLGHVLAAAADAGADEVAVVVGHARERVEPHVAQCLPSARTVVQTEQLGTGHATQIGLTAITAASGTVLVVMGDTPLLQSAALRDLASQHGVGAHAVTVLAARLGDPTGYGRLLVDSATPKVLHELGGRSMLGHVLAAAADAGADEVAVVVGHARERVEPHVAQCLPSARTVVQTEQLGTGHATQIGLTAITAASGTVLVVMGDTPLLQSAALRDLASQHGVGAHAVTVLAARLGDPTGYGRLLVDSATPKVLHELGGRSMLGHVLAAAADAGADEVAVVVGHARERVEPHVAQCLPSARTVVQTEQLGTGHATQIGLTAITAASGTVLVVMGDTPLLQSAALRDLASQHGVGAHAVTVLAARLGDPTGYGRLLVDDLDRPVSATPKVLHELGGRSMLGHVLAAAADAGADEVAVVVGHARERVEPHVAQCLPSARTVVQTEQLGTGHATQIGLTAITAASGTVLVVMGDTPLLQSAALRDLASQHGVGAHAVTVLAARLGDPTGYGRLLVDDLDALAGAAPVDVGGVPTPKVLHAIAGRPLIYFSVRAALDAGCEEVVVVVGHGKQAVADYLEKTFGDQVKTAVQQEQRGSGDAAWIGIQAMTHAAHHVVLWYGDAPLVSAAHLLDVVACSRGLAGGPTLAMATCLHPEPFGYGRVIRNTQGNVICIREEKDLTTDRERAIQEVNVGIYVAPSSFFRKTLPQLKTNNAQRELYLTDIVALGLSH"
  },
  {
    "reference": "MACINAKEVEERLDAYLGVVSSFRLRAVERKSKDLKLLLELEKIIRDTINPKVREIASFMHKFDLDEDTPGNGFRSNIAYTLRYLRKAAKIVCESYPTKSISRMILLLKTQSEILDISLTCEAIADEEHLLFNDRGLAENLALWEKISIIDKTCFYSQMKALHCEGAVRATMDFMLAFTASYSFNRGILGRDVQVLTKVTTSATLPVYALNADLRASKIVTEFQKGDMLFLKDFWNVFETLVMKHVVQIKAPYAGVSRRFHMCSRDDIKLSYKRSVSNMDKESEQDLSTPLETESVEFTVPAPILDQLTVRVIRTQKSSGTFETLPLNKLQAAFMNKIPIVWSKGQAPGVPVIPSADHMERAAKESTSRPGILFHIHGGGFVAQTSRSHQAYLKKWAQQLNVMVVSIDYSLAPEAKFPVALDECFYMYAWVLQHAHQMGHDPNNICISGDSAGGNLTLALGLKIAQEGLPPPKALQATYPAVCMKKVLSPARLNAAADILLPLSVFAIAMGAYIPDDQEKTPSALLSPWHATDDILSKLPPTYIVVGTNDPLLDDSIDFARKLRRAGGEVHLHVADKVTHGFLQLEDTGGVHTRAAAKASLSHLKEMYALPKTSDWTIKQTKYEMGWVRTES",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should contain one or more Hormone-sensitive lipase N-terminal, Alpha/beta hydrolase fold-3 domains that are essential for its function.2. The active site of the protein must be engineered to enable effective catalysis of cholesteryl (9Z-octadecenoate) + H2O = (9Z)-octadecenoate +   cholesterol + H(+).3. A protein that is capable of catalyzing reactions within the Lipid metabolism pathway with high efficiency and specificity.4. A protein that can perform acylglycerol lipase activity, hormone-sensitive lipase activity in caveola, lipid droplet, cytosol to support triglyceride catabolic process, cholesterol metabolic process. The designed protein sequence is ",
    "response#1": "VFTALYDVCEENAAFFSGATKGQAGDAAQRLVDAMTTIQEHARSLEPVISGFAAIYHHFDFDPHIPANGYRSLVKVVRCCLLHIIHKGRYITSNRRSIFFRVSHSATEMEVYCSALCQLRALLYLAQRLLHDNSQGNLFFQEESGISQSFLREYASMHKGCFYGRCVGFQSAVHLSVYLFVFLSGVAASSLLTSGKYAIDPDLRGQEYERITQNLDVHFWKTFWNITETESLVSMTATQVKVNRALSVPPMPFDLPLVADPTHTVTVSPPAAHIGPGPVQMRLISYELREGQTQSLVTLAEDNMAFFSSQGPGETARRLSSVFAGVREQALGLEPALGRLLGVAHLFDLDAETPANGYRSLVHTARCCMAHLLHKSRYVASNRRSIFFRTSHNLAELEAYLAALTQLRALTYYAQRLLATNRPGGLFFEGDEGLTSDFLREYVTLHKGCFYGRCLGFQSLALVHFHGGGFVAQTSKSHLTQSLVTLAEDNMAFFSSQGPGETARRLSSVFAGVREQALGLEPALGRLLGVAHLFDLDAETPANGYRSLVHTARCCMAHLLHKSRYVASNRRSIFFRTSHNLAELEAYLAALTQLRALTYYAQRLLATNRPGGLFFEGDEGLTSDFLREYVTLHKGCFYGRCLGFQSLAVLHFHGGGFVAQTSKSHLVLGTQSLVTLAEDNMAFFSSQGPGETARRLSGVFAGVREQALGLEPALGRLLSVAHLFDLDAETPANGYRSLVHTARCCLAHLLHKSRYVASNRRSIFFRASHNLAELEAYLAALTQLRALAYYAQHLLATNRPGRLFFEGDEGVTADFLREYVTLHKGCFYGRCLGFQFTPAIRPFLQTISIGLVSFGEHYKRNETGLGVTASSLFTSGRFAIDPELRGAEFERIIQNLDVHFWKAFWNITEIEVLSSLANMASATVRVSRLLSLPPKAFEMPLTADPKLMVTISPPLAHTGPGPVLVRLISYDLREGQ",
    "response#2": "VFTALYDVCEENAAFFSGGTKAQAGDTSQRLVDSMTTIQEHARSLEPVITGFAAIYHHFDFDPHVPANGYRSLVKVVRCCLLHIIHKGRYITSNRRSIFFRASHNAAEMEAYCSALCQLRALLYLAQRLLHDNSHGNLFFQEESGLSQSFLREYASMHKGCFYGRCVGFQFTPAIRPCLQSITIGLVAFGENYKKHQSGIGVAASSFFTSGKYAIDPELRGQEYERITQNLDVHFWKAFWNITETQILSSLVSMTATQVKVNRALSVPPEPFDLPLAADPTRMVTVSPPSSHVGLGPVQMRLISHELREGQG",
    "response#3": "VFTALYDVCEENAAFFSGGTKAQAGDTSQRLVDSMTTIQEHARSLEPVITGFAAIYHHFDFDPHVPANGYRSLVKVVRCCLLHIIHKGRYITSNRRSIFFRASHNAAEMEAYCSALCQLRALLYLAQRLLHDNSHGNLFFQEESGLSQSFLREYASMHKGCFYGRCVGFQFTPAIRPCLQSITIGLVAFGENYKKHQSGIGVAASSFFTSGKYAIDPELRGQEYERITQNLDVHFWKAFWNITETQILSSLVSMTATQVKVNRALSVPPEPFDLPLAADPTRMVTVSPPSSHVGLGPVQMRLISHELREGQTQSLVTLAEDNMAFFSSQGPGETARRLSGVFAGVREQALGLEPALGRLLSVAHLFDLDAETPANGYRSLVHTARCCLAHLLHKSRYVASNRRSIFFRASHNLAELEAYLAALTQLRALAYYAQHLLATNRPGRLFFEGDEGVTADFLREYVTLHKGCFYGRCLGFQFTPAIRPFLQTISIGLVSFGEHYKRNETGLGVTASSLFTSGRFAIDPELRGAEFERIIQNLDVHFWKAFWNITEIEVLSSLANMASATVRVSRLLSLPPKAFEMPLTADPKLMVTISPPLAHTGPGPVLVRLISYDLREGQD"
  },
  {
    "reference": "MTQMTSSDVPGMGRRQFMNLLTFGSVTGVALGSLYPVVKYFIPPRASGSGGGTSAKDELGNSVTASGWLANHSDGDRSLVQGLKGDPTYLIVEGDDAIGSYGINAICTHLGCVVPWNSGVNKFVCPCHGSQYNSTGKVVRGPAPLSLALANIAVENDNVFVSQWTETDFRTGEKPWWT",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have a high affinity for [2Fe-2S] cluster.2. The protein must exhibit the following characteristics: Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions..3. The protein contains novel Rieske domains that confer a unique function or activity.4. The designed protein should have 2 iron, 2 sulfur cluster binding, plastoquinol--plastocyanin reductase activity, metal ion binding, electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity within plasma membrane-derived thylakoid membrane to regulate photosynthesis.5. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.6. The protein design should incorporate a binding pocket that specifically recognizes [2Fe-2S] cluster to enhance enzymatic efficiency.7. The protein should have a fast turnover rate for a plastoquinol + 2 H(+)(in) + 2 oxidized [plastocyanin] = a   plastoquinone + 4 H(+)(out) + 2 reduced [plastocyanin]. The designed protein sequence is ",
    "response#1": "MDDTLNQLNPSMSRRQLLNFFTGAIVAATASAAIYPATKFFMPPAEEKQGMRISEASEWLKTHPPGNRTLTQGLKGDPTYLVVENDGTLATYGINAVCTHLGCVVPFNAAENKFICPCHGSQYNNQGRVVRGPAPLSLALAHADIDDGKVVKTGWLATHQAGDRSLVQGLKGDPTYLIVNEVGEIGEFGLNAICTHLGCVVPWDSGANKFICPCHGSQYDTNGKVVRGPAPLSLALAHVDIEDDAVLNHPAGDRSLVQGLKGDPTYLIVDGDAAIGNFGINAICTHLGCVVPWNSGANKYICPCHGSQYDANGKVVRGPAPLSLALTHIDIDDDNVLYDNAESI",
    "response#2": "MDDTLNQLNPSMSRRQLLNFFTGAIVAATASAAIYPATKFFMPPAEPAPPAAEPSKPASEWLKTHPPGNRTLTQGLKGDPTYLVVENDGTLATYGINAVCTHLGCVVPFNAAENKFICPCHGSQYNNQGRVVRGPAPLSLALAHADIDDGKVVGHPIPASQILVQASGTRALIAGLAGEPTYLTVREDGTLDPMGIVNNCTHLGCTFPWNPVDQQFQCPCHGSRYDAQGSVERGPANRPLKLVHVQVKDDYIWNHPAGDRSLVQGLKGDPTYLIVDGDAAIGNFGINAICTHLGCVVPWNSGANKYICPCHGSQYDANGKVVRGPAPLSLALTHIDIDDDNVLYGHPIPASQILVQASGTRALIAGLAGEPTYLTVREDGTLDPMGIVNNCTHLGCTFPWNPVDQQFQCPCHGSRYDAQGSVERGPANRPLKLVHVQVKDDYIWNHPAGDRSLVQGLKGDPTYLIVDGDAAIGNFGINAICTHLGCVVPWNSGANKYICPCHGSQYDANGKVVRGPAPLSLALTHIDIDDDNVLYDGETF",
    "response#3": "MDNSIPIESPSLSRRQLLNFITGATVAVTAGAALYPAGKFLIAPNGKVVRGPAPLSGAVECLSNGKVVRGPAPLSGAVECLSGGPASGLQGSVERGPANRPGTLECLSGGPASGLSGSSASPQGSVERGPANRPGTLECLSGPPSGLSGSPECLSGGPASGLSGSSASLRQGSVERGPANRPGTLECLSGPPSGLSGSPECLQGSVERGPANRPGTLECLSGPPSGLSGSPECLSGSPAGEPTYLIVKEDHTLDRIGLVDNCTHLGCTFPWNPLDQQFQCPCHGSRYAPDGSVVRGPAPLPLGMLECLSGGLPAGLSGSPACLSGGPASGLSGSSECLSGAPASGLSGSPECLSGSSASGLSGSLECLSGGLPAGLSGSPACLSGGPASGLSGSSECLSGGLPAGLSGSPACLSGGPASGLSGNLECLSGGLPAGLSGSPACLSGGPASGLSGSSECLSGGLPAGLSGSPACLSGGPASGLSGSLECLSGGLPAGLSGSPACLSGGPASGLSGSSECLSGGLPAGLSGSPACLSGGPASGLSASLECLSGGLPAGLSGSPACLSGGPASGLSGSSECLSGGLPAGLSGSPACLSGGPASGLSGSLECLSGGLPAGLSGSPACLSGGPASGLSGSLECLSGGLPAGLSGSPACLSGGPASGLSGNLECLSGGLPAGLSGSPACLSGGPASGLSGSLECLSGGLPAGLSGSPACLSGGPASGLSGNLE"
  },
  {
    "reference": "MASSYRLVLGTGNPGKVDELQALLADLPIDLIPASSLEVPPEVVEDADTLEGNAQKKAETFHRHSGLPALADDTGLEVAALDGRPGVHTARFAGPDATPDDNKRKLLEVLDGVEDRRARFRTVVALVDEDAVHTFEGVCEGTITTAPRGDGGFGYDPLFQPDGFKQTFAEMPPDAKNEISHRRKALDALRSFLADTD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..2. The protein must exhibit ribonucleoside triphosphate phosphatase activity. The designed protein sequence is ",
    "response#1": "MPLKLNT",
    "response#2": "MGLND",
    "response#3": "MRSCCAN"
  },
  {
    "reference": "MKLVLATHNQGKVQEIMELLRGFNVTVLSLSDYPNVGDITEDGATFAENAVKKACFVAEQTGEVVMADDSGLEVDYLNGAPGVHSARFAGAETNDHANNEKLLKLLSGVPPEMRTARFRCVIAVTEPEGRVSIAEGTCEGIITNEPRGSGGFGYDPLFYLPTHGKTFAELDLATKNKISHRSKAVNKIAFILSAVIKKNIIITNRQ",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.2. The Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.3. The protein design must be able to perform ITP diphosphatase activity, XTP diphosphatase activity, nucleotide binding, metal ion binding, ribonucleoside triphosphate phosphatase activity in order to contribute to nucleotide metabolic process, purine nucleoside triphosphate catabolic process.4. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MXDXKLVIATNNXNKIKELKDXLXDXFELXTXSXLNXVPEVPETIEXGNTXXQNAIKKAEXVSKSTGXIAIADDSGLEVDCLNGAPGIYSARYSGEGASDXANIKKLLENLEKIXPEFRNAKFVCVAAXVMXNGEXFFXEGILNGKILXEPRGNNGFGYDPIFXVPEFGKTSAELSSXEKNNISHRGIALKKLKRLXG",
    "response#2": "MDNIEIILASNNAGKLREFDKMLKPLGYKLKSQNDFNVPDAEETGLTFVENALIKARHAAVVSGLPALADDSGLCVNALNGEPGVYSARYAGENASDSENNKKLLKELSGVLNFYDKTAFYTCVLVYMRHTDDPSPIICQGIWEGEILEEPRGENGFGYDPLFYVPHYKKTAAELTLEEKNNISHRGKALRELLEKFS",
    "response#3": "MAPRPIVVASGNPGKIREIAAMLAPLEIEVVPQSAFNVSEAEEPHPSFVENALAKARHASRVTGLPALADDSGLAVDALGGAPGIHSARYAGENATDAENNLKLLRELRGVPREKRAARFVCVLVLVRHADDPLPIVCHGVWPGEILAAPRGTGGFGYDPLFWVPERGCSAAELPADLKNALSHRGRALRQLLAELPRL"
  },
  {
    "reference": "MPLPKIDDWRELSDEEISEQILATKKELFELRLQKATRQLEKPHLVRHAKHKLAQLMLLESQRTAAAKEK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have structural constituent of ribosome and be active in cytosolic large ribosomal subunit. The designed protein sequence is ",
    "response#1": "LREYKVVGRLLPSAKNPTPPLYRMRIFAPNHVVAKSRFWYFVSQLRKMKKANGETVYCGLVHEQSPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTSGAVTQCYRDMGARHRARAHAIQIMKVR",
    "response#2": "LREYKVVGRLLPSAKNPTPPLYRMRIFAPNHVVAKSRFWYFVSQLRKMKKANGETVYCGLVHEQSPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTSGAVTQCYRDMGARHRARAHAIQIMKVL",
    "response#3": "LREYKVVGRLLPSAKNPTPPLYRMRIFAPNHVVAKSRFWYFVSQLRKMKKANGETVYCGLVHEQSPLKVKNFGIWLRYDSRSGTHNMYREYRDLTTSGAVTQCYRDMGARHRARAHAIQIMKVG"
  },
  {
    "reference": "MYTREELMEIVSERVKKCTACPLHLNRTNVVVGEGNLDTRIVFVGEGPGEEEDKTGRPFVGRAGMLLTELLRESGIRREDVYICNVVKCRPPNNRTPTPEEQAACGHFLLAQIEIINPDVIVALGATALSFFVDGKKVSITKVRGNPIDWLGGKKVIPTFHPSYLLRNRSNELRRIVLEDIEKAKSFIKKEG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Removes uracil bases that are present in DNA as a result of either deamination of cytosine or misincorporation of dUMP instead of dTMP.2. The target protein must exhibit helix-helix-strand-helix-helix-helix-strand-helix-helix-strand-helix-turn-helix-helix-strand as its primary conformation.3. The protein should be designed to have high stability and activity towards Hydrolyzes single-stranded DNA or mismatched double-stranded   DNA and polynucleotides, releasing free uracil..4. The designed protein should have a high specificity for uracil binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "FKFTPFNDVKVVIIGQDPYHGPNQAHGLAFSVMDGTKLPPSLRNMYKELEDDLNIQRHTGELTGWAYEGVLLLNNVLTVVGGQAHSHKGIGWEQFTTEIVKAVSHYRENVVFILWGAPAQKLESLIDRSKHKVIKSVHPSPLSAYRGFFGSKPYSQTNEYL",
    "response#2": "FSYPFDAVKVLIMGQDPYPTPGHAMGLSFSTQPDVRPLPRSLNNIFKELVSDVGSLGDSASEQGALDLGINAPGSVAGTQVALPADGDLRAWSNQGVALFNRVLTVHPGQAGSHKGKGWEAVTEQAIKALAERDQPLVAILWGKQAQEVQKFLGDTPCICSVHPSPLSASRGFFGSKPFSRANEIL",
    "response#3": "KYTAFEDVKVVILGQDPYHGPNQAHGLAFSVKPEVAIPPSLLNIYKELTQDISGFQMPSNGYLVKWAEQGVLLLNTVLTVERGMAHSHANLGWERFTDKVIAVLNEHREKLVFLLWGSHAQKKGQMIDRTRHLVLTAPHPSPLSAHRGFFGCRHFSKTNSYL"
  },
  {
    "reference": "MTLQRSSCLVLGLALLQLVHRVASACPSACQCPLEVPRCAPGVGLVLDGCGCCKVCAKQLNEDCSKTQPCDHTKGLECNFGASVTAPKGICRAQSEGRPCEYNSKIYQNGESFQPNCKHQCTCIDGAVGCVPLCPQELSLPNLGCPNPRLVKIPGQCCEEWVCDNSKDALEDLDGFFGKDFGVDVSEGELTRKNELIAIVKEGLKMMPVFESEPRSHSFENPKCIVQTTSWSQCSKTCGTGVSTRVTNDNPSCRLVKETRICEVRPCGQPSYDSLKKGKKCTKTRKSQAPVKFTYAGCSSVKRYRPKYCGSCVDSRCCTPQQTRTVKIRFRCDDGETFTKNVMMIQSCRCNYNCPHTNDAYPNYRLFNDIHKFRD",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Incorporate a signal peptide in the protein design.2. A protein that can perform extracellular matrix binding, heparin binding, integrin binding in extracellular matrix, extracellular region to support positive regulation of cell-substrate adhesion, positive regulation of transcription by RNA polymerase II, positive regulation of osteoblast differentiation, osteoblast differentiation, atrial septum morphogenesis.3. The presence of IGFBP N-terminal, VWFC, CTCK domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "QLDIVIVLDGSNSIYPWESVTAFLNDLLKRMDIGPKQTQVGIVQYGANVTHEFNLNKYSSTEEVLVAANKIGRRGGLQTMTALGIDTARKEAFTEARGARRGVKKVMVIVTDGESHDNYRLKQVIQDCEDENIQRFSIAILGHYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVTIVKALGERIC",
    "response#2": "IDGPTNVVTNQVTEDTASVSWDPVRADIDKYVVRYISHDGETKEKAVPKDQSSTVLTGLKPGEAYKVFVWAERGNQGSC",
    "response#3": "QECSGQCHCPEVPPQCSPGVSLVLDTCGCCRVCAKQLGELCTERDVCDPHKGLYCDYGSPSNRRIGVCTC"
  },
  {
    "reference": "MAASSGSPQLATEDHLRKGEAVSGLHAVVAGSVSGLVARSVTAPMDTVKIRRQLQLASEHKYHGILHTFRTVAREEGVRALWKGNVPASAMYVLYGSLQFGTYAWLNTAAASAGLPPQAHSLAVGALAGLVSSLLTYPLDLLRTRLVANRSAHFFSLRRQARVIWDTEGPAGFFRGGAWAIAATTLTTGLIFGIYETCTIAADTYGLPWLAAAASPTAGLVSKAAVFPLDTVRRRLQIVDAKHIPFFTRDPGAYSALRGTRFLGLAVHMVRAEGIASLYKGLTMALCKSTPTTVITLWVYQRCLRLLEPTRAPQLPA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria..2. A protein with thiamine pyrophosphate transmembrane transporter activity functions is required to enable mitochondrial thiamine pyrophosphate transmembrane transport.3. The designed protein should have at least Helical; Name=3, Helical; Name=6, Helical; Name=4, Helical; Name=5, Helical; Name=1 transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "LERLAALLNGAGRVTILAGRGCRMAHAALVTLAERLQAPVVHALGGKEFVEYDNPYDVGMTGLIGFSSGYDAMMGCDTLLMLGTDFPYRQFYPTKAKVAQIDLRPENLGRRTGIDIGLVGDVGPTIQAR",
    "response#2": "EKLVEVLNGAKNITLMCGSGCAGAHDEVVKLAETLKAPVVHALRGKEHIEWDNPYSVGMTGLIGFSSGYHAMMNADTLILLGTQFPYRAFYPTDANIIQIDINPGSIGAHCQVDMALVGDIKTTLTAVK",
    "response#3": "IETLAQRLNGSSKITLLCGAGCADAHDEVVALAAKLQAPIVHALRGKEYLEYDNPYDVGMTGLIGFASGYHAMRQAETVLLLGTSFPYRAFYPEKANILQVDNNPASLGRHCQIDFGVLGDIKHTIDED"
  },
  {
    "reference": "MRVRETQMNWPNLWKWGTLILGLVIICSASDNLWVTVYYGVPVWKDADTTLFCASDAQAHETEVHNIWATHACVPTDPNPQEIPLENVTEKFNMWKNNMVEQMHEDVISLWDQSLKPCIKLTPLCVTLNCTKVNATNVSIKNNTNATNVPIKNNTSDIGKITDDMMNCTFNMTTEIQDKKQKVHALFYKLDIVGIGSNRSDNDSSYRLINCNTSVIKQACPKVSFDPIPIHYCAPAGYAILKCNDKNFTGKGQCKNVSSVQCTHGIKPVISTHLLLNGSLAEEDIIIRSENITDNGKNIIVHLNKTIKINCTRPSNNTRTSIRIGPGQAFYKTGEIIGDIRQASCEINGTEWNKTLTQVSEKLKKHFKNNTIIRFQPPSPGGDLEITMHHFNCRGEFFYCNTTQLFNESGGNGTITLPCRIKQIINMWQGVGQAMYAPPVRGKISCVSNITGILLTRDGGGGDTNGTETFRPAGGNMKDNWRSELYKYKVVQIEPLGLAPTKAKRRVVDREKRAAIGAMIFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQRLLGLWGCSNKIICTTNVPWNTTWSNKSLEEIWDNMTWTQWEREISNYTNQIYDILTESQTQQERNEKELLEFDKWASLWNWFDISRWLWYIRIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTLSHHQREPDRPEGTEEGGGEQGRDRSVRLVSGFLALAWDDLRSLCLFS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.2. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.3. The protein must exhibit the following characteristics: Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state.4. The designed protein have Retroviral envelope protein GP41-like domains with specific sequence motifs that are crucial for its activity.5. The designed protein should have at least Helical transmembrane domains to ensure proper localization.6. The protein design should include a YXXL motif; contains endocytosis signal motif to ensure proper function.7. The designed protein should have structural molecule activity within plasma membrane, viral envelope, host cell plasma membrane to regulate positive regulation of receptor clustering, fusion of virus membrane with host plasma membrane, virion attachment to host cell, positive regulation of establishment of T cell polarity. The designed protein sequence is ",
    "response#1": "MRVKGIQKNWQHLWTWGTMLLGLLMICSASENLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPSPQEIILKNVTENFNMWKNGMVDQMHEDVISLWDQSLKPCVKLTPLCVTLVCTNVNVSSNSSEKIKISSDLKEEMEELWVTVYYGVPVWKEAETTLFCASDAKSYVTEKHNVWATHACVPTDPNPQEIVLENVTENFNMWKNSMVEQMHQDIISLWDQSLKPCVMLTPLCVTLKCSNISRKTNNSGTIETEIQNEIKNCSFNMTTELRDKHQKVHALFYRLDIVPINDNETNLNNSEYRLINCNTSAIKQACPKVTFEPVPIHYCAPAGFAILKCRNKKFNGTGTCNNVSSVQCTHGIKPVVSTQLLLNGSLAEENIIIRSENITDNTKVIIVQLVEPVMINCTRPNNNTRTGVRIGPGQTFYATGDIIGDIRQAQCNINGTKWNETLEKVAGELRKHFNNKTIVFRPSSGGDLEVTTHTFNCGGEFFYCNTSSLFNSTWDENNTKSAQGPNGTANITIPCKIRQIVRMWQRVGQAMYAPPIPGVINCISNITGLILIRDGGNESATNETFRPIGGDMKNNWRSELYKYKVVKIEPLGVAPTRARRRVVQREKRAVGLGAVFIGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTTVPWNESWSNKTYETIWENMTWMDWEREIENYTNEIYTLIERSQNQQEKNEQELLALDKWANLWNWFDITNWLWYIKIFIMIVGGLIGLRIIFTVLSIVNRVRQGYSPLSFQ",
    "response#2": "MIGEIKNCSFNITTAVRDRVQKQYALFYTLDIVPISTENENYTSYRLVSCNTSVITQACPKISFEPIPIHYCAPAGFAILKCNNQTFNGKGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEVVVRSENFTNNAKTIIVQLNESIEINCTRPNNNTRKSIHIGPGRALYAQDIIGDIRQAHCNISSTKWNNTLGQVVKKLREQFGNKTIIFNQTTGGDPEIVMHSFNCGGEFFYCDSTKLFNSTWYFNGTWNNTNNNNDTHDPITLPCRIKQIVNMWQEVGKAMYAPPIKGQIRCSSNITGLILTRDGGVDNSSTNETFRPGGGDMRDNWRSELYKYKVVELKPLGIAPTRAKRRVVQREKRLLLGAMFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARILAIERYLKDQQLLGLWGCSGKLICTTNVHWNSSWSNKSEKDIWDNMTWMQWDREINNYTDTIYRLLEESQNQQEKNEQDLLALDNWKNLWSWFSITNWLWYIKIFIMIVGGLIGLRIILGVLSIVRRVRQGYSPLSFQTLTPNPREPDRLGGIEEEGGEQDRDRSVRLVNGFLALAWDDLRSLCLFSYHRLRDFI",
    "response#3": "MIGEIKNCSFNITTAVRDRVQKQYALFYTLDIVPISTENENYTSYRLVSCNTSVITQACPKISFEPIPIHYCAPAGFAILKCNNQTFNGKGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEVVVRSENFTNNAKTIIVQLNESIEINCTRPNNNTRKSIHIGPGRALYAQDIIGDIRQAHCNISSTKWNNTLGQVVKKLREQFGNKTIIFNQTTGGDPEIVMHSFNCGGEFFYCDSTKLFNSTWYFNGTWNNTNNNNDTHDPITLPCRIKQIVNMWQEVGKAMYAPPIKGQIRCSSNITGLILTRDGGVDNSSTNETFRPGGGDMRDNWRSELYKYKVVELKPLGIAPTRAKRRVVQREKRXLGAVFLGFLGVAGSTMGAASLTLTVQARQVLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLRARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNTSWSNKSLNEIWDNMTWMDWEREIDNYTSLIYTLIEESQIQQEKNEQELLELDKWASLWNWFTITNWLWYIKMFIMIVGGLVGLRIVFVVLSIVNRVRQGYSPLSFQTRLPPQREEGRPEGIEEGGGERDRDTSGPLVDGFLALIWVDLRSLCLFSYHRLRDLLLI"
  },
  {
    "reference": "MLKYAIIFAVISLIAGALGFGGVAAGAAGIAKILFGLFLILAVIFVVLAALGVGAVRKGMK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a specific function within the plasma membrane. The designed protein sequence is ",
    "response#1": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPQ",
    "response#2": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPC",
    "response#3": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPC"
  },
  {
    "reference": "MLTETLTMKYTATKFRVRTNKRLRSQKYPEKHSDFLGCSSTKSSFGLNMLLTKPNKFQIPPPHPVLLKRIREERMKLTSSFESGINIIDIETSWEIDKFISHHFNTSIGNVLKSKSSSKKRPMNAFMAFRTYYAQLGTGLKQNTLSVILSEAWNAPETDQNIWDIFAQQFNFASPRCGFVNYIMAHASSAP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design must be focused on enhancing the efficiency of positive regulation of mating-type specific transcription, DNA-templated.2. The protein must exhibit the following characteristics: Mating type proteins are sequence specific DNA-binding proteins that act as master switches in yeast differentiation by controlling gene expression in a cell type-specific fashion. The designed protein sequence is ",
    "response#1": "IVKTFADLAEDDREAAMRAFSRMMRRGTEPVRRIPAAKKKVNGFMGFRSYYSPLFSQLPQKERSPFMTILWQHDPFHNEWDFMCSVYSSIRTYLEQEKVTLQLWIHYAVGHLGVIIRDNYMASFGWNLVRFPNGTHDLERTALPLVQHNLQPMNGLCLLTKCLESGLPLANPHSVIAKLSDPSYDMIL",
    "response#2": "MVQLMRCIKEPAAQAAFTAKLLVASPAVSGQPATPEKARKALNAFVGFRCYYITIPMFKPWPMKKLSNLIGLLWEADPNKSLWSLMAKAWSTIRDQIGKDQAPLNQFFRIICPHLKLPDPASYLEIHGWILNVNEEGDPTISRSADSEFASIGTGNTDMTLSVEDIITYVQSLGYAHGFILDDNKPSSTFLGHSVL",
    "response#3": "MVQLMRCIKEPAAQAAFTAKLLVASPAVSGQPATPEKARKALNAFVGFRCYYITIPMFKPWPMKKLSNLIGLLWEADPNKSLWSLMAKAWSTIRDQIGKDQAPLNQFFRIICPHLKLPDPASYLEIHGWILNVNEEGDPTISRSADSEFASIGTGNTDMTLSVEDIITYVQSLGYAHGFILDDNKPSSTFLGHSVL"
  },
  {
    "reference": "MLEGNGTELRLSEFGFERRFDERGAIEWMQANWSKSFLFSAIYATLVFGGRHYMKHRPKMNLRRPLVLWSLSLALFSIIGAMRTGSYMVHVLSGSGFRQSICDQSFYNGPVSKFWAYAFVLSKAPELGDTVFVVLRKQKLLFLHWYHHITVLLYSWYSYKDMVAGGGWFMTMNYGVHALMYSYYAARAAGLRVPRPLAALITGAQIGQMVMGLAVSGLVYRWMRHGDCPSHLDNCAWAALMYLSYLLLFSNFFYQTYLRPKTTKAE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle; This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle2. The designed protein should be optimized for very-long-chain 3-ketoacyl-CoA synthase activity activity in order to improve very long-chain fatty acid biosynthetic process. The designed protein sequence is ",
    "response#1": "GLLFLHWYHG",
    "response#2": "GLIFLHWYHG",
    "response#3": "GLLFLHWYHG"
  },
  {
    "reference": "MKSNKKSNRLRAIYRALVIAIGLAVIIVFNYFNRKNNNARSSRRACSCFFSLTGVNLEKIGTFDTDAKLIVLNHQSLLDIIYLEAYHPRNICWIAKKELGEIPFYGHALTDTGMILIDREDKKGIVSLLKACKEKLDQNRPLVIFPEGTRGKGGEKFLPFKQGAKIIAEKFQLKIQPMVLINSIKIFNSKPLEAYKARTRLVMLESYTPDFNSPTWYEELQERMQKEYLKHYHELNPSEQ",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein have enhanced a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-   sn-glycero-3-phosphate + CoA.2. The designed protein should be optimized for 1-acylglycerol-3-phosphate O-acyltransferase activity activity in order to improve CDP-diacylglycerol biosynthetic process. The designed protein sequence is ",
    "response#1": "LCQGL",
    "response#2": "GYVI",
    "response#3": "GVAA"
  },
  {
    "reference": "MSSEFLAELHWEDGFAIPVANEENKLLEDQLSKLKDERASLQDELREYEERINSMTSHFKNVKQELSITQSLCKARERETESEEHFKAIAQRELGRVKDEIQRLENEMASILEKKSDKENGIFKATQKLDGLKCQMNWDQQALEAWLEESAHKDSDALTLQKYAQQDDNKIRALTLQLERLTLECNQKRKILDNELTETISAQLELDKAAQDFRKIHNERQELIKQWENTIEQMQKRDGDIDNCALELARIKQETREKENLVKEKIKFLESEIGNNTEFEKRISVADRKLLKCRTAYQDHETSRIQLKGELDSLKATVNRTSSDLEALRKNISKIKKDIHEETARLQKTKNHNEIIQTKLKEITEKTMSVEEKATNLEDMLKEEEKDVKEVDVQLNLIKGVLFKKAQELQTETMKEKAVLSEIEGTRSSLKHLNHQLQKLDFETLKQQEIMYSQDFHIQQVERRMSRLKGEINSEEKQALEAKIVELRKSLEEKKSTCGLLETQIKKLHNDLYFIKKAHSKNSDEKQSLMTKINELNLFIDRSEKELDKAKGFKQDLMIEDNLLKLEVKRTREMLHSKAEEVLSLEKRKQQLYTAMEERTEEIKVHKTMLASQIRYVDQERENISTEFRERLSKIEKLKNRYEILTVVMLPPEGEEEKTQAYYVIKAAQEKEELQREGDCLDAKINKAEKEIYALENTLQVLNSCNNNYKQSFKKVTPSSDEYELKIQLEEQKRAVDEKYRYKQRQIRELQEDIQSMENTLDVIEHLA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in extracellular region, axoneme, cytosol, cilium that is critical for cilium-dependent cell motility, determination of liver left/right asymmetry, cerebrospinal fluid circulation, regulation of cilium beat frequency.2. The protein must exhibit the following characteristics: Probably acts together with CCDC40 to form a molecular ruler that determines the 96 nanometer (nm) repeat length and arrangements of components in cilia and flagella ; Not required for outer dynein arm complexes assembly.; Required for assembly of dynein regulatory complex (DRC) and inner dynein arm (IDA) complexes, which are responsible for ciliary beat regulation, thereby playing a central role in motility in cilia and flagella.3. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "LNIEGLKYKERDGFEMAFTFWQPQPWNVFEVTAYWEQRSRALLGTRYDHRNGAFDWDLSMTLKERGGQQICSQEYRYWRETGVAFVFPEYEHCKPNKTLAVGLVRNGRNFIHRGYVGDIQTGPFCGFGLRTVEERMHHSVHGDNDYRATDITERNLMEFFHELLTQTAYEHDTTRSRRYGSVQLLMTPLLNHQEEDAAGQAAYDKPWIHVPGVTVHFVSPMEMEQLKKGAAHWNNMFDIVFMAYNYYSFLSKDFFQAMRAQSLFILETKLMTVERKDRVQEYESKAKELMKDAGLKN",
    "response#2": "YYPAGVQRPGLGCRELIFRNLSKILPAISRDVTNWVAGTRIKSSQLLAVLLLHGEDHITQHMELLLSTLYHSCLDEENQVVINSVKSAELIGTFVNPEVFLKLILSALQKSPLASHLMTLAACIRGCSREMLKTHLTKITSVISQSDVCQGSERVLYQEQLLCCIQSLIDVCQQDCTEVSLQCMNVLITILG",
    "response#3": "RERCEELRVELLSIHQKKVCEERKAQIAFNEELSRQKLVEEQMFSKLWEEDRLAKEKREAQEARRQKELMENTRLGLNAQITSIKAQRQATQLLKEEEARLVESNNAQIKHENEQDMLKKQKAKQETRTILQKALQERIEHIQQEYRDEQDLNMKLVQRALQDLQEEADKKKQKREDMIREQKIYHKYLAQRREEEKAQEKEFDRILEEDKAKKLAEKDKELRLEKEARRQLVDEVMCTRKLQVQEKLQREAKEQEERAMEQKHINESLKELNCEEKENFARRQRLAQEYRKQLQMQIAYQQQSQEAEKEEKRREFEAGVAANKMCR"
  },
  {
    "reference": "MADMVATLEAVDILGLMKLLPHRYPFLMIDRIVDIDGDESAVGIKNVTINEPHFQGHFPDQPVMPGVLIVEAMAQTAGAICIRSLATEKPSLVYFLTIDNAKFRKPVVPGDQLRIHVKKIKKRGNLLKFACEALVDGAKAAEAEISAMMVTRPDNRNAHKIQTSIHPSSVVEEGAQIGEGVRVGPFCHIGADAVIGDRVELVSHVSVMGATTIGAATKVYPMAILGAPPQNTKHKGGRTTLVIGENCTIREGVTMHVGTDSSRGETTVGDNGNFLAYAHIAHDCVVGKNATFANGATLGGHCEIGDNVYIGGLSAVHQFVRVGDNAFLGGCSAFVGDVIPYALAVGNRASLRGLNIIGLKRSGVPRAEIYLLRKAYRMIFDRSRTVAENVELAKAEFASSPTAMKIIDFITGRGKRHYAVPSLKGGDGDDADDEG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A key design requirement for the protein is the optimization of the active site to achieve high catalytic efficiency for a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O.2. For general function, the protein need meet that Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.; Involved in unsaturated fatty acids biosynthesis3. A protein that contributes to fatty acid biosynthetic process, lipid A biosynthetic process by functioning in cytoplasm with acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity, 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity, 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity, (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity, 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity. The designed protein sequence is ",
    "response#1": "MNSDVKNILIVGSGPIXTXAXAXAXRXLSXFNXKXILXXTXPXAXNDXCSXEXFXPXLXEGXFXKILKKTXXLTEPDXVXXALGGQXALXIAKXLXSXLXKKXPXXIKISNXELXPXXTXFXEIXPXFXNXIXNXXLXTCIGXXNXXXYXIDXXXNXSXXXIXGXXKXHLXXYXRXHXXXGXXXCIXPXYXNXNXXXNXLDXHXFXPXXXXXXNXXXWXXXSXVXGSXXXVXXXNXDXSXXXSXXXNXXXQHXQXHXXMXXXMXXXPXXXHXXMLXXXNXHXMIHSXXYXMHXXX",
    "response#2": "MPNDRDTATTRRLSAFTIDQLAARGGVRSDAVPAALLLDQLGDPHSAYRAVRADDVWRPCPTAFPATQPDADASGPTAAPTDAAGPAVASADLIRFHGVLRTEQDAVLAASADADCRMLPDHAAVTDPHGAELTARTRLTAVAAGLTGAAPADSLGTNGSANGSTASLAGADGADGAGPVGAGRGAGPEDDGAVAAGRLVTGAKRWCSLAGLLSAHAVGVAVDGPDQRRVFAVVDLSGTGIRRVHDDPLLGVPGDPVCELFLDDVAVPADAVLGGPGFGAVLPTFETPEARIGAAAASVGLGAAALDSAVEYTAHRVQFGQAIGDFQAVKHRVADMYVQAEAARSAAWFAAWRAAEGAADGGGAIAVAAAKARAAHAAVDVCRSAVQLHGGIGFTWEHDLHLYLKRRICDRVLFGDPREHLAELAALV",
    "response#3": "MGSLGKGLTAQRLASQGIAIGTVSAFGASTITQDIAGVFKDGKIQYKTVDVNTPDKVEQLVSEIKADYGRLDVLVNNAGIATESTLESLNEENINKTFDVNVKGVIYGIRAAAPVMRKQKSGHIINLSSLSAIRPVANLSLYNSSKFAVEGFSESLALEVKPWNVKVTLVEPGGFKTEWAGNSLDPTKDIKASEEYENLQSKMDNFKQAMDGSQPGDPAKGAEVILKVVDAPNPPLRYPVGTNAFMLANLRSRLGPKFEEIVDFNKTLGSADDLSTFK"
  },
  {
    "reference": "MRLKEFLTLEEVEEAQGDLNQEVSGLAYDSRNVGPGQIFFAIPGEKLDGHDFIPQAIEREAGAVVVAKKHACPPGTASIRVRDVRRIMGLWSAHFFGWPSRQLKLVGVTGTNGKTTVTYLVESIFSAAGMEAGVIGTISYRYRGREVPSHHTTPESLDLEALLAEMHQAGTQAVAMEVSSHALAQERVRGLEFDVAVFTNLSRDHLDYHADMDDYFLAKSRLFTDHLKNGSKPKRAAVICGEDPHGKELLKTVRDQGLEVWSYGEGAQWDIHPLYVRSDVQGLKGKLELKGQIFDFSSPLIGSANLQNIMGAAGVGLALGVSAQVVSEGIQRLTAVPGRLEKVENDLGISLLVDYAHTPDALEKVLGAVRPLTRGKVLAVFGCGGDRDRGKRPLMGEIAARLSDLVVLTSDNPRTEEPLAILHEIEMGVRKTGLTKFPVPSSGSSRGYCVEADRREAIRMALRLSRPGDVVLIAGKGHEDYQILGEKKIHFDDREVARQELGRIATT",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein contains novel Mur ligase C-terminal, Mur ligase central domains that confer a unique function or activity.2. The protein should have a fast turnover rate for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.3. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.4. The protein requires Mg(2+) to activate a critical catalytic site.5. The designed protein should be able to compete with endogenous proteins or inhibitors for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP binding.6. The protein design must feature the Meso-diaminopimelate recognition motif motif.7. A protein that is capable of catalyzing reactions within the peptidoglycan biosynthesis, Cell wall biogenesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MKLSRIEGISCDSRTVRPNYLFVAIKGERNDGHLFIKEALNRGASALLVERRIEGVNVPYVMVEDTRAALGQVASNFYQNPSEKLTLVGVTGTNGKTSVATFTRQIWMALGHAAINIGTTGVEGAWAAPSSHTTPDPITLQAMLAEAAAAGVTHAAMEASSHGLDQRRLDGVRLKAAGFTNLTQDHLDYHHTMEAYADAKAALFTRLLPEDGTAVVNLNGAGGAEMLALAEERGLKTLAIGRDPLADLRITGQRYDATGQDLRFDWKGEPFQLHLNLVGGFQAENVLVAAGLAIVTGVAPQVAADALAVLTGVPGRMNRLGGAGRPTVVVDYAHTPDALEQALASLRDHVRGRLLCVFGCGGERDRGKRAQMAAIAERLADAVVVTDDNPRGEDGDAIVAAILEGFANQDTVTVQRDRAAAIAHAIGAAGPGDIVLVAGKGHEPGQIVGDQVLPFDDATVAREALRG",
    "response#2": "MKLQDIVRNTAVIDVQGNTNIEITGVEYDSRRVREGSLFVAMRGGTTDGNKFIRQAMDKGAGGVLTDSRDYPEPCLIVSNTRKALALAAENFYGNPSKDLLLIGITGTNGKTTTSYLTKSIIEASGKKAGLIGTIKNIIGDQELPAKRTTPESYDLQKLFSQMVDEGVEYCVMEVSSQALKMHRVDGFTFDIGIFTNLEPDHIGPNEHKDFNDYMACKGLLFKQCRLGIANIDDEHFAEILKGTTCKIETYGFSSKADVRASNLHLESSGIRFELNTFEGNISIDVPLPGLFNVSNSLAAISVALQLGVSEDDIKQGLASVKVKGRLEQVETGRDFHVVVDYAHKPDAVEAALSTLRPLTAQAGGRLICVIGAGGDRDTGKRPVMGEIAARLADLVIVTDDNPRSEDPAVIRAAVLEGTRDGAGEVREVGDRREAIRVALSSAGPGDIVLVAGKGHEDYQEIKGVKYPMDERVLIKEILEELGHK",
    "response#3": "MKLKELMAGLTYPVMEITGVEYDSRRVREGSLFVAMRGGTTDGNKFIRQAMDKGAGGVLTDSRDDIEGISCDSRTVRPNYLFVAIKGERNDGHLFIKEALNRGASALLVERRIEGVNVPYVMVEDTRAALGQVASNFYGYPAKKLQVIGITGTNGKTTTSYLTKSIIEASGKKVGLIGTIANYIGDEKIKSVRTTPESADLQRLLAEMVDAGCECVVMEVSSQALMLDRVYGCEYDVGVFTNLTRDHLDFHGTMENYCDAKKMLFARCSRAIVNIDDEYGRRIIEESAALETYGFSEKADVRAKNVKYSASGISFDIHTADGTYEADISLIGKFNVYNALAAIGAVLALGIDMDQVKAGMKAVKGVKGRMNRLGGAGRPTVVVDYAHTPDALEQALASLRDHVRGRLLCVFGCGGERDRGKRAQMAAIAERLADAVVVTDDNPRGEDGDAIVAAILEGFANQDTVTVQRDRAAAIAHAIGAAGPGDIVLVAGKGHEDYQILKDKTIHFDEREIAADILREMSK"
  },
  {
    "reference": "MTKEVWNTERLHDHFAQSLRVSEMYYDSKTEHQRLKVFQNGTFGRVLTLDDVVQTTEGDNFIYHEMLTHVPILAHGAAKRVLIIGGGDGGIAREVLKHASVEHVTMVEIDAGVVDFSKEYLPMLSQGAFDDARLNLVINDGAVFMKETEDKFDVIIVDSTDPIGPGEVLFTDTFYGHAARALTEDGIIVTQNGVPFMQGDELTNTMRAFQALFQDATCYLATIPTYVGGPMALGWGSHSTKARSVDLTTLEARFAAAGLSPDYYTPEVHKAAFALPGYVKKLFP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a high specificity for spermidine, S-methyl-5'-thioadenosine binding, with minimal off-target effects.2. The presence of PABS domains is critical for the interaction of the designed protein with its intended targets.3. The protein design should be able to enhance the efficiency of spermidine biosynthetic process.4. A protein that is capable of catalyzing reactions within the spermidine biosynthesis, spermidine from putrescine: step 1/1 pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "PGPISLIEPLSGNSALLVKINAVYVVKRSRYQEIVVADTEDFGRALILDDYIQSSYYDEVYYHESLVHPAMSTHPSPADVLILGGGEGATLREVLKHRTVKRAVMVDIDGDVVEVARRYLPQMHQGAFDDPRAQVVIEDGFVYVERALAAGDKFDVVIMDLTDPYSSDIAKQLYAPGFFKKVRGLLREDGLVVTQAGNSFFFPEAYDMVLHGVRSSFPTVAEYSVWIPSFGYAVNYILGSTYNGGKHK",
    "response#2": "SRGHRARQLTEPVGRGLSRSWEVSEVLFETRTEFQHLLIGRTAQGISLFCDGERQSTEATQLTYHEALMVPPMLLAARVRRVLIIGSSEGVASRLAVAEGAEIVDHVDIDQVAVRACAEHLPYGYTPAELAAAERGDGQVRVHYRDGWEFLAGAEPGYDVVVIDLPDENDDPSAQHNRLYGTEFLARCTALLAPGGVVSSQAGCPTMWRNETLISAWRRFTGAFGTVVYYGSDEHEWAFLSGRVAPPRSVTEPISDGLTRHWRLGAVHHVADTGFQRVVVADTAHGVTLFCGDERQSAEHTQLAYHEALVWPGALLARELRRVLIIGSSEGVACEMALDAGAEHVDHVDIDAEAVRICAEHLPYGYTPGTLAAAERGDGPVRLTYGDGRQYVLDATEQWDLVVVDLPDERPDEPAAQINQLYAEDFVRACAQRLTPGGVLVFQAGSPAVWRDATLRSAWQRFRSVFEPSGGRGVYVGCEEHEWAFLAGVP",
    "response#3": "MWITQEITPYLRKEYTIEAKLLDVRSEHNILEIFKSKDFGEIAMLNRQLLFKNFLHIESELLAHMGGCTKKELKEVLIVDGFDLELAHQLFKYDTHIDFVQADEKILDSFISFFPHFHEVKNNKNFTHAKQLLDLDIKKYDLIFCLQEPDIHRIDGLKRMLKEDGVFISVAKHPLLEHVSMQNALKNMGGVFSVAMPFVAPLRILSNKGYIYASFKTHPLKDLMTPKIEALTSVRYYNEDIHRAAFALPRSVTEPISDGLTRHWRLGAVHHVADTGFQRVVVADTAHGVTLFCGDERQSAEHTQLAYHEALVWPGALLARELRRVLIIGSSEGVACEMALDAGAEHVDHVDIDAEAVRICAEHLPYGYTPGTLAAAERGDGPVRLTYGDGRQYVLDATEQWDLVVVDLPDERPDEPAAQINQLYAEDFVRACAQRLTPGGVLVFQAGSPAVWRDATLRSAWQRFRSVFEPSGGRGVYVGCEEHEWAFLAGVP"
  },
  {
    "reference": "MQRVNMIMAESPGLITICLLGYLLSAECTVFLDHENANKILNRPKRYNSGKLEEFVQGNLERECMEEKCSFEEAREVFENTERTTEFWKQYVDGDQCESNPCLNGGSCKDDINSYECWCPFGFEGKNCELDVTCNIKNGRCEQFCKNSADNKVVCSCTEGYRLAENQKSCEPAVPFPCGRVSVSQTSKLTRAETVFPDVDYVNSTEAETILDNITQSTQSFNDFTRVVGGEDAKPGQFPWQVVLNGKVDAFCGGSIVNEKWIVTAAHCVETGVKITVVAGEHNIEETEHTEQKRNVIRIIPHHNYNAAINKYNHDIALLELDEPLVLNSYVTPICIADKEYTNIFLKFGSGYVSGWGRVFHKGRSALVLQYLRVPLVDRATCLRSTKFTIYNNMFCAGFHEGGRDSCQGDSGGPHVTEVEGTSFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKTKLT",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa..2. The designed protein should contain one or more EGF-like domains that are essential for its function.3. A key design requirement for the protein is the optimization of the Charge relay system to achieve high catalytic efficiency for Selective cleavage of Arg-|-Ile bond in factor X to form   factor Xa..4. Incorporate a signal peptide in the protein design.5. A protein that can perform calcium ion binding, endopeptidase activity, serine-type endopeptidase activity in collagen-containing extracellular matrix, endoplasmic reticulum lumen to support zymogen activation, proteolysis. The designed protein sequence is ",
    "response#1": "VVGGLVALPGSHPYIAALYWGDSFCAGSLIDPCWVLTAAHCLQKRPAPEELTVVLGQDRHNQSCERCQTLAVHSYRLHEGFSSKTYQHDLALLRLRGRKNSCAILSPHVQPVCLPSSAAPPSETVLCEVAGWGHQFEGAEEYATFLQEAQVPFISLDRCSSSNVHGDAILPGMLCAGFLEGGADACQGDSGGPLVCDEGVTERQLTLRGVISWGSGCGDRNKPGVYTDVANYLDWIQEHTL",
    "response#2": "VVGGLVALPGAHPYIAALYWDQHFCAGSLIAPCWVLTAAHCLQNRPAPKELTVVLGQDRHNQSCEQCQTLAVRDYRLHEAFSPITYQHDLALVRLQESADGCCAHPSPFVQPVCLPSTAARPAESEAAVCEVAGWGHQFEGGEYSSFLQEAQVPLIDPQRCSAPDVHGAAFTQGMLCAGFLEGGTDACQGDSGGPLVCEDETPERQLILRGIVSWGSGCGNRLKPGVYTDVANYLAWIARTIAAST",
    "response#3": "VVGGLVALPGAHPYIAALYWDQHFCAGSLIAPCWVLTAAHCLQNRPAPKELTVVLGQDRHNQSCEQCQTLAVRDYRLHEAFSPITYQHDLALVRLQESADGCCAHPSPFVQPVCLPSTAARPAESEAAVCEVAGWGHQFEGGEYSSFLQEAQVPLIDPQRCSAPDVHGAAFTQGMLCAGFLEGGTDACQGDSGGPLVCEDETPERQLILRGIVSWGSGCGNRLKPGVYTDVANYLAWIREHTC"
  },
  {
    "reference": "MARRQATTKKRRTSTRKKKPTVAAKRQMTGNVIGLIGLLVTILALLKVGLVGMVFAEFFRAIAGNAYQIFAGLTLLVMGYLMIFGRWPRLTWRWWVGGNLFFFSYLLLLEIPMFNQLNHHTDFWSLTWNLIKNDFAKGGMASNLGGGLVGAAGYSVTYPLLANVGTILIALILMVASIYITFDLPFHKTMQALRQALMQLGQQLRSGYEWLTHVLRVQIARWRVHQQVRANQAASTEKQPTSTVPQSAAPAETTSGTSAPDSAASAVTSSPADLNITVASDREASPIKSPTSAATVDHEQELQGTEVMDDADYQLPESTLLTKIPKTDQSAEYATIESNSQKLTTTLASFGVQVEVKNVSLGPSVTKYELHPAVGVKVSKVVNLADDLALALAAKDLRIEAPIPGKSLIGIEVPNKQISTVSFRDIVEAQPAHPTKPLAVPLGRDVSGNLVVADLSKMPHLLIAGSTGSGKSVAINVMITGLLMNTKPSQVKFMLIDPKKVELGVYNGIPHLLTPVVTEPKKAARALHKVVAEMERRYELFADSKQRNMQGYNQYIRQQNAADGQSRPVLPYIVVVVDELADLMMVTSSEVEDAIIRLGQMARAAGIHMILATQRPSVDVITGLIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLYQPMGMNKPLRVQGAYISDHDVEEVVNFIKAQQTADYDDSMLVKDDETDAAGSGDPRDGEDEYYAEAVELVTDQQSASVSMLQRRFRIGYNRAARIVDEMEERGVV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be involved in cell division, chromosome segregation by localizing to plasma membrane and exhibiting DNA binding.2. The presence of FtsK domains is critical for the interaction of the designed protein with its intended targets.3. The protein should have a Helical domain that is long enough to span the entire membrane.4. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.5. The protein must exhibit the following characteristics: Required for activation of the Xer recombinase, allowing activation of chromosome unlinking by recombination .; The N-terminus is involved in assembly of the cell-division machinery. The designed protein sequence is ",
    "response#1": "KMPHLLIAGATGSGKSVCINSLIVSLLYKASPKQVKMILIDPKVVELNIYNGIPHLLTPVVTDPKKAAGVLNWAVQEMIRRYSLFADHGVRDIESYNEKYKEERLYKIVIIIDELSDLMMVSPAEVEEYIFRLAQMARAAGIHLVIATQRPSVDVITGVIKANIPSRISFAVSSQIR",
    "response#2": "RLKECCLILGVLTSAFLSIALLTFSPADPSWSQTSWGGDISNAGGQFGAWVADTLFFTFGLLAYLLPVLLVIVTWVFFRTRDEDEHIDLMLWGTRLLGLAILILTSCGLADINFDDIWYFSSGGVLGDVLNSLALPTLNLLGTTLVLLFAWGAGFTLLTGISWLSIVEWIL",
    "response#3": "TMPHLLVAGATGSGKSSFVNSMITSILLRAHPSQVRLVLIDPKRVELAIYSGVPHLITPIVTDPKKASEVLQWVVKEMERRYDDLASFGFRHIDDFNLAVRAKKIASDSRELTPYPYLLVIVDELADLMLVAAKDVEESIVRITQLARASGIHIVLATQRPSVNVVTGLIKANVPSRLAFAVSSLVDSRVILDRPGR"
  },
  {
    "reference": "MFRGKIFVLSLFSIYVLSSAAEKNTSFSALFAFGDSVLDTGNNNFLLTLLKGNYWPYGLSFDYKFPTGRFGNGRVFTDIVAEGLQIKRLVPAYSKIRRISSEDLKTGVCFASGGSGIDDLTSRTLRVLSAGDQVKDFKDYLKKLRRVVKRKKKVKEIVSNAVFLISEGNNDLGYFVAPALLRLQSTTTYTSKMVVWTRKFLKDLYDLGARKFAVMGVMPVGCLPIHRASFGGVFGWCNFLLNRITEDFNMKLQKGLTSYAVEYDFKDAKFVYVDIYGTLMDLVKNPMAYGFTEAKKACCCMPNAIIPCFHPDKYVFYDFAHPSQKAYEVISKPIVYQIAKGLA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The Nucleophile, active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The designed protein should be involved in lipid catabolic process by localizing to pollen coat and exhibiting lipase activity.3. For general function, the protein need meet that Required for the formation of pollen coats and male fertility. The designed protein sequence is ",
    "response#1": "MSPSSGPSGAPDCMANLMNMTGCLSYVTVGEGGGAAKPDKTCCPALAGLVESSPQCLCYLLSGDMAAQLGIKIDKAKALKLPGVCGVITPDPSLS",
    "response#2": "MSPSSGPSGAPDCMANLMNMTGCLSYVTVGEGGGAAKPDKTCCPALAGLVESSPQCLCYLLSGDMAAQLGIKIDKAKALKLPGVCGVITPDPSLF",
    "response#3": "MSPSSGPSGAPDCMANLMNMTGCLSYVTVGEGGGAAKPDKTCCPALAGLVESSPQCLCYLLSGDMAAQLGIKIDKAKALKLPGVCGVITPDPSLI"
  },
  {
    "reference": "MTRFIFITGGVVSSLGKGIASASLAALLEARGLDITMIKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIRSTMGRNNNFTTGQIYDSVIRKERKGEYLGGTVQVIPHITNQIKESVLLGADDNDICLVEIGGTVGDIESLPFLEAIRQMGVELGPQRVMFMHLTLVPYIKASGEIKTKPTQHSVKELRSIGIQPDILVCRSEQPLPENERKKIALFTNVPEKAVISAVDADSIYRIPVLLHAQEMDELVNQRFNLDLPPADLAEWEAFLEGLDSLAEEVKIAMVGKYVHLTEAYKSLSEALVHAGVHTGNRVVIHYVDSEQIERSGTESLEGMDAILVPGGFGNRGVEGKVTAVQYARENRIPYLGICLGMQVAVIEFARHVAGLEGAHSTEFKRDTPHPVIGLITEWLNADGNVETRNEESDLGGTMRLGGQQCQLEPNSKTHHLYGQEVITERHRHRYEFNNSYLDNIVTAGMKVSGRSVDGRLVEIVEIPEHPWFVACQFHPEFTSTPRYGHPLFSGFIEAARKHRDGTA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of CTP synthase N-terminal, Glutamine amidotransferase domains is critical for the interaction of the designed protein with its intended targets.2. A protein with metal ion binding, glutaminase activity is needed to enhance 'de novo' CTP biosynthetic process, glutamine metabolic process in a specific context.3. The protein should be able to bind ATP, L-glutamine, CTP, UTP ligand in a variety of conditions4. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen5. The protein's Nucleophile must be carefully engineered to ensure that the amino acids involved in H2O + L-glutamine = L-glutamate + NH4(+) are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "MFVTGGVCSSLGKGIATASIGAIMKAAGLKVFVQKLDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDEPMSRLSTVTTGRIYSDVIAKERRGDFLGGTIQVVPHITNAIKHSIISAAEQSGTEIMMVEIGGTVGDIEGEPFLEAVRQMRGELGPQRIFHVHLTLLPYLKGSKELKTKPTQLSVRELRRIGLQPDMILARADYRVPKDLLEKISRFCGVPSEAVIPALTVNSIYDVPLNFQKYDVARIIGEKLSIPLPDAPEGEDTSWRRFVESHSDTAGEVKIGLVGKYVELADAYKSIIEALIHAATYNDRKAKIELIHSESLNNENVAEKLKEMDGILVAPGFGHRGIDGKLVAVKYARENNIPFLGICLGMQCAVIEFARDVLKYDNANSREMDHSTPYPVIDLMEDQKNVTDMGGTMRLGAYDCTLTKNTNSHKAYASEHTKERHRHRYEFNNEYLKAFEAAGMKPAGCNQETGLVEIMEIPSHKWFVGVQFHPEYSSTVLKPHPIFMAFLEAALVHS",
    "response#2": "MFITGGVVSSLGKGIAAASLAAVLEARGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFVCMTATRRNNFTTGRIYENVIRKERRGDYLGGTVQVIPHITDEIKRCIRLGAGEADIAMIEIGGTVGDIESLPFLEAIRQMGVELGRERSLFIHLTLVPFIKSSGELKTKPTQHSVKELRSIGIQPDILMCRSDREIPAEERRKIALFTNVEPKAVINVPDADTIYRIPLLLRAQGVDDIVARKLQLQDAYLSVIESLKIAGWEFNKKVKIKWIQADKLNEQNYKEVLKESQAILVPGGFGNRGIEGMMLASRFARENDIPYLGICLGMQIATISMARDLLGWKDANSTEFDETTSHAIFDYIRGIDRHNIGGTLRLGTMATKLEKDSIAQKLYNSDNAYERHRHRYEFNNEYKKDLESVGLRFSGIYEEQNLVEIVEIPKLKFFVASQFHPEFTSRPNKPSPLFKGFISWINSVKESYKSLSEALVHGGIANNCRVNLKYVDSESLERHGINATFDDVDGILVPGGFGERGSEGKIAAIGHAREQRIPFFGICLGMQMAVVEYARNVCGIEDAHSSEFHDDAENPVIHIMEEQKRVRGKGGTMRLGAYPCVLGEGTLARRIYGTDQISERHRHRYEFNNAYRERLQECGLVLSGINPDSDLVEIVEMKDHPWFLGCQFHPEFKSRPMDPHPLFESFIQAAINR",
    "response#3": "MFITGGVVSSLGKGIAAASLAAVLEARGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFVCMTATRRNNFTTGRIYENVIRKERRGDYLGGTVQVIPHITDEIKRCIRLGAGEADIAMIEIGGTVGDIESLPFLEAIRQMGVELGRERSLFIHLTLVPFIKSSGELKTKPTQHSVKELRSIGIQPDILMCRSDREIPAEERRKIALFTNVEPKAVINVPDADTIYRIPLLLRAQGVDDIVARKLQLTDAYKSLNEALLHAGIHAHTRVNIHYIDSERVERDGTACLEHMDAILVPGGFGERGIEGKIAAVRFAREHRLPYLGICLGMQVAVIEFARHVAGLEGAHSTEFRKDTPHPVIALITEWIDEDGSVQQRREGGDLGGSMRLGAQPCRLVADSLAGATYGKDVIVERHRHRYEFNNRYRGPLGAVGMRFCGHSLDGRLVEMIELPDHPWFLACQFHPEFTSTPRAGHPLFRGFLSATRAAVEIAVVGKYVALADAYLSVVEALAHAGIRHGVEVDIRWVDAHELASPALTERALAGAHGVLVPGGFGLRGIEGKIAAARHARERHVPYLGICLGLQVAVIEFARHVLGLSEANSSEFDPDTPDAVVDLMADQRDVRDLGGTMRLGVYPCKVTEGTRTHAAYGEPVVYERHRHRWEVSNRYRQRLADAGMRFAGLSPDDRLVEIIELAEHPWFVASQFHPEFRSRPNRPQPLFREFLAGAS"
  },
  {
    "reference": "MAREATRIRRRERKNITSGIVHVNSSFNNTMVTITDAQGNTISWSSSGVMNFKGSRKSTPYAAQMAAEDAAKKAKEHGLQTVEVRVRGPGSGRESALRALQAAGLTVTAINDTTPIPHNGCRPPKRRRV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be designed to localize specifically to ribonucleoprotein complex in order to perform its role in translation more effectively.2. The protein must exhibit the following characteristics: Forms part of the Shine- Dalgarno cleft in the 70S ribosome.; Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. The designed protein sequence is ",
    "response#1": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#2": "MHKPTGS",
    "response#3": "VLPW"
  },
  {
    "reference": "MKTIAVLGSTGSIGTNTLDVVRRNRHLYEVYSLVAGQNIELLTGQILEFRPKLAVVASAAVLDLLTASLQAAGLPKSEWPDLLSGDAARVAAVRAPEVDTVISAIVGVAGLEATYEAVCLGKRVGLANKEVLVSGGSLVMEAVRKFGAELLPVDSEHNGAHQCLRAGNRAQVSRLILTASGGPFRNTPVSELPFVTPGQALNHPTWKMGNRITIDCATLMNKGFEVIEACWLFDFAPRDVGVVIHPQSTVHAMIEYSDGSVLAQISATDMRMPIQYALTYPDRADAPVPKIDWAEARKWEFLPPDLEKFPLLKLAYQCQESGGSATCILNAADEIAVEAFLQGRIGFLSIHEIVQETLSRMPSRTPASVGDILEIDRESRTLARELANCRAAGTVTA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a low Km value for 2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-   xylulose 5-phosphate + H(+) + NADPH.2. The NADPH binding site should be located in a region of the protein that is accessible to the ligand.3. A protein that can modulate the overall flux of metabolites through the isopentenyl diphosphate biosynthesis via DXP pathway pathway, enhancing or suppressing its activity as needed.4. The designed protein should be optimized for NADPH binding, metal ion binding activity in order to improve terpenoid biosynthetic process.5. The protein must be engineered to have a high affinity for Mn(2+) in order to increase its catalytic activity.6. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4- phosphate (MEP).. The designed protein sequence is ",
    "response#1": "MGEPDMATPIALTLSYPERVKAGVKPLDFTQVGELTFLQPDFERYPCLALAIEACYLGQHATTTLNAANEVAVAAFLARQIKFTDIARVNDSVLNQVCKQSLASGLDSLESLLELDRMARHYGDPTAKIRTIGLYPVDSEHSAVFQSMEGQSRKDVLRIILTASGGPFLNLPLDQLSRVSIDDALNHPNWSMGRKITIDSATMMNKGLEVIEARWLGYNVGLMTTVHNRFPGEIKTPIADVVNTTPEVRLMDLLPIDSEHNAIFQSLPEKVQSQLGYSSLSDNGVSRIILTGSGGPLRETPLVQFADVTPDQACAHPNWSMGRKISVDSATMMNKGLEYIEARWLTGNDESPPDMRLPIALGLGWPDRVPRAAQACDWTQATSWTFEPLDTVAFPAVDLAKDAAKQGSTFPAVFNAANEEAVEAFHAGRIRFTQIVDTVESVLSEHSGSSELTVESVLDAEKWARLIAE",
    "response#2": "MLGSTGSIGTQTLEIVSELPDKFKVVALSAGRNIDLLTEQVSQHKPEVIAIEDENLLTDLKNNINNLGISNPPIVLGGREAINSVAAWDSADTVITGIVGCAGLIPTMSAIKAGKNIALANKETLIAAGQLFVDAAKAAGVKILPVDSEHGAIFQCLRGNKKEEVKNIIVTASGGPFRGKKKEELIDVKPEHALKHPKWNMGRKISIDSATLMNKGLEVIEAHFLFNLLPADSEHSAIFQCLQGTPWPENARLSTGIPTPGLRNIQLTASGGAFRDWATEDLKHASIKDATSHPNWSMGKKITVDSATLMNKGLEVIEAHYLFNASAEQIEVVVHPQSVVHSMVSFQDGSVLAQLGVPDMKAPIAYALTYPERVPTGVNPLDLTALSGLTFFKPDYRRFPALKLAYDALAAGESMPAVMNAANEVAVEAFLSGVIGFIDIAATIARIMDAHEAHTLSTIEEALITDRWARAHAAQMV",
    "response#3": "MLGSTGSIGTQTLEIVSELPDKFKVVALSAGRNIDLLTEQVSQHKPEVIAIEDENLLTDLKNNINNLGISNPPIVLGGREAINSVAAWDSADTVITGIVGCAGLIPTMSAIKAGKNIALANKETLIAAMGEPDMATPIALTLSYPERVKAGVKPLDFTQVGELTFLQPDFERYPCLALAIEACYLGQHATTTLNAANEVAVAAFLARQIKFTDIARVNDSVLNQVCKQSLASGLDSLESLLELDRMAREKGVKILPVDSEHGAIFQCLRGNKKEEVKNIIVTASGGPFRGKKKEELIDVKPEHALKHPKWNMGRKISIDSATLMNKGLEVIEAHFLFNMATPDMKLPIQYALNYPNRKESQIEPLDFYKISNLTFEKPDMDTFLPLKLAYEAGKKGGVMPAILNGANEVAVDLFLKGKIEFLQIGDLLQECMNKFYKSMEATLENVISVDKEVREMLGSTGSIGTQTLEIAKECPEQFKVVALTAGRNLDLLIKQIQEHQPEVVALARKDILPELQERLNSLTGKERPNALPHLVAGNDGLNIAASWHSADLVVTGIVGCAGLLPTLAAIKAGKDIALANKETLIAALGAPDMRIPIAHCLGFPDRITGRAAKLDLAKIGQLTFEAPDFTRFPGLRLAYDALRTGNGATTVYNAANEVAVAAFIAQKIRFGAIARLVEDTLNSWVRAGNQAPLGSADDAIALDQQARELGLSLQLGAPDMRIPIAHCLGFPDRITGRAAKLDLAKIGQLTFEAPDFTRFPGLRLAYDALRTGNGATTVYNAANEVAVAAFIAQKIRFGAIARLVEDTLNSWVRAGNQAPLGSADDAIALDQQAR"
  },
  {
    "reference": "MPTIQQLIRSSRKKITKKTKSPALKMCPQRRGVCSRVYTTTPKKPNSALRKVARIKLTSGFEVTAYIPGIGHNLQEHSVVLIRGGRVKDLPGVRYHIIRGTLDTSAVKDRIRSRSKYGVSK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: With S4 and S5 plays an important role in translational accuracy; Located at the interface of the 30S and 50S subunits ..2. The designed protein must possess structural constituent of ribosome to support translation under certain conditions. The designed protein sequence is ",
    "response#1": "MRRRTAEKRNIVPDPIYGDILLSKFINRLMYDGKKSLAQQIVYSALERLAEATKENPIEAFHKAIDNVKPVVEVRSRRVGGSTYQVPFEVPENRATSLAIRWIVTSAQSKKGRDMVSKLSQELIDAYNNTGPSVKKKEDVHRMAEANR",
    "response#2": "EINGEIRAHEVRLTGLDGEQIGVVSLKEALEKAEEAGADLVEISPNAEPPVCRIMDYGKFLYEKSKTLKEG",
    "response#3": "MRRRTAEKRNIVPDPIYGDILLSKFINRLMYDGKKSLAQQIVYSALERLAEATKENPIEAFHKAIDNVKPVVEVRSRRVGGSTYQVPFEVPENRATSLAIRWIVTSAQSKKGRDMVSKLSQELIDAYNNTGPSVKKKEDVHRMAEANL"
  },
  {
    "reference": "MAEVKVEVASIDWQKRCLSLETQLFRFRLQASKIRELLADKMQELEQRLLEAEQRAENAETQVGVMEEKIKLSNLKSVDSTGTLHQKYQELLRAVQGKDELISQLQAQLEKQKQTRAEEAKIVQEKAAKIKEWVTVKLAELEMENQQLKTCNQQLVEQVAALQDALEDLRMTPSEELLVVPEGTPERDPVPSGPSDQPVEQDSNPHTQILKVAVPTPSLGTLQSRDSLSEARSLEDLRFSMVHPGETAEAKTLQSHLQKEGSPSQLCMKPGNPKHGSASYRESLVTAQGGTFPGTKTSAREGGPGSSLTLPKVRAPSIPRDSFQVAKRHHSQPQVGPGHCDHVVSIEIGALSALHSPGSSKSEARAKVREEAEKMEMEALPPSGKQEERESLKSRRGELEDVELENKPPTPPLHRFPSWESRIYAMATSGMQLSEVSSRRSNAACYASGPSALAFPGAFSGLVYKNVTVPVYTALKGKATQISNVPFVDESSGSDDDCGSQASFRMSVPCSEYRKTSGLGSPRAIKRGVSMSSLSSEGDYAIPPDACSLDSDYSEPEHKLQRTSSYSTDGEALEKSGYLLKMGSRVKTWKRRWFVLRQGQILYYKSPSDVIRKPQGQVDLNSHCQIVREEEAQTFQLISGNKTYYLTAESPSLLEEWIRVLQSLLKVQVTGPPALHQGGTKPTVKGWLTKVKHGHSKLVWCALVGKTFYYYRSHEDKRPLGCLPVQDAHIEEVDRSCDSDEDYEAGGTGRLLSSHCTLVIHPPEHSPT",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein have PH, MyTH4 domains with specific sequence motifs that are crucial for its activity.2. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.3. The designed protein localizes to the cytoskeleton. The designed protein sequence is ",
    "response#1": "MCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLWPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNSLMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGC",
    "response#2": "YTKAPIQESLLSLSDDVSKLAVASFLALMRFMGDQSKPRGKDEMDLLYELLKLCQQEKLRDEIYCQVIKQVTGHPRPEHCTRGWSFLSLLTGFFPPSTRLMPYLTKFLQDSGPSQELARSSQEHLQRTVKYGGRRRMPPPGEMKAFC",
    "response#3": "MCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLWPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNSLMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGY"
  },
  {
    "reference": "MPVDLNFTIAAPLLALAAGALLILLLDLLFRYETIQGPMYVAAVGAVLVAGWYLVPLWRGAAEPFGFHGMLVMDRFAAVYGLVLLGAALLAILLSFGRVREDQSGYLALLLWAAMGMVLLGGAGNLMVIFLGIELLSLALYVMIAFAPKRMAAREAAFKYFVLGSVAAAFLIFGFALIYGAAGTMSLTGIAAAARSFTSEGAWSVGLYYKVGVGLAIVGLAFKMALVPFHIWAPDVYQGAPTPVTAFMAIGTKAAAFAAMARLLVAAVPQAYQPSFLLPLSILAFASMMLGATVGIWQSDLKRLMAYSGIANAGYLIMAIPGLGLDGLSAAAYYLAAYGFATMGVFAVVRILEADGVDGSQLANLKGLFYRSPWVGVCLAVLFFGLIGVPPTGGFVGKFLLAIAAVRGGAWIVLTGLILSTGISAYVYLKVIGTAFTRTKVAPQPEEGEEPHPPTRTAAQVVLAIATAGTLVLGVLPGPVSELLRVALAGM",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain The designed protein sequence is ",
    "response#1": "RLDHPYIRKNGKLVEVSWSEALKTIMDKIKSVKPEKIAAIAGSIVSVEAMFMLKILLQKLGSNNYTVNQFNYKIDTSERGNYLFNTTIVGVEKADLCLLIGANIRQIAPILNSRIGRRVRIGALKVVRIGIGHNQTYKIHDLGNDIKIIEDLAVGTHEYTKAFKEAKYPMIIVGDGVYGRDDGYALLSLIHKVVDKYNMMRDDWQGFNILHNHASIVGGLDIGFNTAIKFEGVKLAYLLGADAIPFDKLKSAFIIYQGHHGDVGAMSADVILPSAAYTEQSGIYVNLEGRPQIAQKAVSPVGRAKEDIEIIKEIANL",
    "response#2": "RLDHPYIRKNGKLVEVSWSEALKTIMDKIKSVKPEKIAAIAGSIVSVEAMFMLKILLQKLGSNNYTVNQFNYKIDTSERGNYLFNTTIVGVEKADLCLLIGANIRQIAPILNSRIGRRVRIGALKVVRIGIGHNQTYKIHDLGNDIKIIEDLAVGTHEYTKAFKEAKYPMIIVGDGVYGRDDGYALLSLIHKVVDKYNMMRDDWQGFNILHNHASIVGGLDIGFNTAIKFEGVKLAYLLGADAIPFDKLKSAFIIYQGHHGDVGAMSADVILPSAAYTEQSGIYVNLEGRPQIAQKAVSPVGRAKEDIEIIKEIADAFG",
    "response#3": "RLDHPYIRKNGKLVEVSWSEALKTIMDKIKSVKPEKIAAIAGSIVSVEAMFMLKILLQKLGSNNYTVNQFNYKIDTSERGNYLFNTTIVGVEKADLCLLIGANIRQIAPILNSRIGRRVRIGALKVVRIGIGHNQTYKIHDLGNDIKIIEDLAVGTHEYTKAFKEAKYPMIIVGDGVYGRDDGYALLSLIHKVVDKYNMMRDDWQGFNILHNHASIVGGLDIGFNTAIKFEGVKLAYLLGADAIPFDKLKSAFIIYQGHHGDVGAMSADVILPSAAYTEQSGIYVNLEGRPQIAQKAVSPVGRAKEDIEIIKEIAEILS"
  },
  {
    "reference": "MENRWQVMIVWQVDRMRINKWKSLIKYHMYISKKARGWFYRHHYESNHPRISSEVHIPLGEGEGELVVTTYWGLHTGEREWHLGQGVSIEWRMKRYRTQVDPGLADQLIHMHYFDCFSESAIRKAILGHIVSPKCEYQAGHNKVGSLQYLALTALIKPQKRKPPLPSVQKLTEDRWNKPQRTRGHRENRTMNGH",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G; Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions; In turn, they are directed to the 26S proteasome for degradation2. Incorporating a BC-box-like motif motif is critical for the successful design of the protein.3. The designed protein should be expressed specifically in the host cell cytoplasm The designed protein sequence is ",
    "response#1": "MPQLDTSTWFTILFSLTFMFMLTLPKLTSQTN",
    "response#2": "LETEGM",
    "response#3": "MRVKGIRKNYQRLWRWGTLLLGMLMICSATEQVXT"
  },
  {
    "reference": "MTGAPWYHRDISRVRAEELLAHAGIDGSFLVRDSESVPGAYALCLLFQRHVHTYRILPDADGLLAVQATQGVQVNCFRTLGDLVLGYQNPNKGLVAPLLYPVMRESEANDESSDGDDEKPGSTFANSPPRAISPTATSPPSSSAAPHLLLQRLQELSPNSVGCEIVSLLDEYLHGNVCKDLENLKGGASGLQHFQGILSHICDSLHSEIDQTLSSLETLAKVFDHPSGHLTFSTMQNIGKSPEENMDNLLQKLTTLCNLLSSLEKRVLKALQEAVANHNLSLQPATPPESSRAPKKLARPNPVHSFQVKVVRYGRQTVSVDINEGVLLFDKKSGSFGVETVTLDRIVQLVKFQRGPAKLKMVVDSHHNPPRELVFESTKKREAFCRLLQLMKTTHSQKSELDVISVFVGTWNMGGTPPPGSLQSWVTCSGLGLTPDESISSLPHDIYAVGTQDNPQGEREWAEHIRATLRTSTNIDYKQVAVQSLWNIRLAVFVKPEHEARISQVNTASVKTGLGNTLGNKGAVGVSLLFNGTSMGFVNCHLTSGSDKALRRNHNFQDILRLLSLGEKQLSTFDISLRFNHLFWCGDLNYRLDLDALDILKHVSKREFEELMCADQLTRERHKRKAFFNFKEEKIMFPPTYRYERGSRDCYLWQKYKSSGVQVNGPSWCDRVLWKSYPESHVICTSYGCTDDIFTSEHSPVFATFEVGVISLFPRTESCSERASIELEAIEAIVKTSSKAKFFIEFHSRCLEEPRRSAENDSQYCEVP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design must feature the NPXY motif motif.2. The designed protein must have at least one SAM domain.3. For general function, the protein need meet that Required for the maintenance and dynamic remodeling of actin structures as well as in endocytosis, having a major impact on ligand-induced EGFR internalization and degradation; Participates in regulation of cortical and submembraneous actin; Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways4. We need a protein that can perform a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-   trisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-   inositol-3,4-bisphosphate) + phosphate with high efficiency. The designed protein sequence is ",
    "response#1": "LPDDFKANTKIKVNNHLFNKENLPSHFEFKEYCPQVFRNLRERFGIEDLDYQASLARSAPMKGDGQGEGLLFTSYDRTLIVKQISSEEVADMHNILSEYHQHIVKCHGSTLLPQFLGMYRITVESEDTYLIVMRNMFSHRLLVHRKYDLKGSLVDREASDKEKVKELPTFKDMDFRNNMQKVYVTEEQKEKMMEKLNRDVEFLVKLKIMDYSLLLGIHDVARGEREEEEAEEPCYEDDADPENGLAPALQVGSYGTSPEGIAGYMNSIKPLGPGEFDPYIDVYAVKSAPGAPQREVYFMGLIDVLTQYDTKKKAAHAAKTVKHGAGAEISTVHPEQYAKRFREFISNIFS",
    "response#2": "GDGQGEGLLFTSYDRTLIVKQISSEEVADMHNILSEYHQHIVKCHGSTLLPQFLGMYRITVESEDTYLIVMRNMFSHRLLVHRKYDLKGSLVDREASDKEKVKELPTFKDMDFRNNMQKVYVTEEQKEKMMEKLNRDVEFLVKLKIMDYSLLLGIHDVARGEREEEEAEEPCYEDDADPENGLAPALQVGSYGTSPEGIAGYMNSIKPLGPGEFDPYIDVYAVKSAPGAPQREVYFMGLIDVLTQYDTKKKAAHAAKTVKHGAGAEISTVHPEQYAKRFREFISNYRPVALLFHKMMFETIPMFSGGTCSKYTAFSCISCEVTLCFCHADPQFVVYQLKVKIFTSPSGPSRREDKYMYFEFPQPLPVCGDIKVEFFHKQNKMLKKVCFLAFLENIVNSTLELGHRLFFGLDENSDKVENGSLVADQELDGIFSTERSDNDKEYLILTLTKNDLDKANKDKANRYFSPNFKVKLFFTGVLPERIYQGSSGSYFVKDASHQCLAVFKPKDEEPYGRLNPKWTKWMHKLCCPCCFGRACLIPNQGYLSESGASLVDRKLNLNVVPKTRVVRLVAESFNYARIDRQKAKLKKRIKEHYPSAHFNRMSLPLKTGSFQLFVEGYKDADYWLRRFESEPLPPSVAKSFQLQFERLVVLDYIIRNTDRGNDNWLIKYVAPKITTQVGGTGGRTMHGVVTPKLPNALGASKIVDLNEPPQASPKPTTGGRPSNHLAGGAATSAQQDSSSLASSEDEPSSRSPPTSEPDWNVVNSAFIRIAAIDNGLAFPFKHPDSWRAYPYHWAWLPQAKIPFSEEIKEQVLPQLSDMNFVEEICTELLYLFQQDRGFDKRLFERQMSVMRGQILNLTQALRDGKSPVQLVQMPAVIVE",
    "response#3": "MAKGLGTGLLHTPIKNIFVNVKFQSGGESFTFQISPNEFPITLMSYAVKKQATVFRHETMENPEDYTLQVNGKYEYLYGNYPLYQFQYIRSCLHRGLTPHLTMVHSSPILFKVGDDLRQDQLVIQIIILMDRLLQKENLDLKLTPYRILATNATAGAVQFIPSTSLSAVSAKYKSVLAYLQANNPDENEPLGVRKETMDTYVKSCAGYCVITYLLGVGDRHLENLLLAPDGHGGYLYRPVALLFHKMMFETIPMFSGGTCSKYTAFSCISCEVTLCFCHADPQFVVYQLKVKIFTSPSGPSRREDKYMYFEFPQPLPVCGDIKVEFFHKQNKMLKKVCFLAFLENIVNSTLELGHRLFFGLDENSDKVENGSLVADQELDGIFSTERSDNDKEYLILTLTKNDLDKANKDKANRYFSPNFKVKLFFTYKPIALLFHKMLFETIPMFTGGTCSDVLCSPADPQFVVYQLKVKIHTSTPTHTRREDKLMLFEFPQPLPVCGDIKVEFFHKQNKVMKKEKMFHFWVNTFFIPGPEENLDRMENGSAGTLRELTDRTHQNLAMTTAGSETNDREYLILTLTKNDLDKANKDKANRNFSPNFKVKLYFTGIQ"
  },
  {
    "reference": "MGQKINPVGLRLGINRTWDSRWFADGPEYGRLLHEDIKVRRALKKRLYQAGVSRIIIERPHKKCRITIYAARPGVIIGKKGADIEKLRKDVAAMTDGEVHLNIVEIRKPETDAQLIAENIAQQLERRVAFRRAMKRSMQSAMRLGAKGVRINVSGRLGGAEIARMEWYREGRVPLHTLRADVDYGFTEAKTTYGVIGVKVWVFKGEVLEHDPMALDKRLAGESGPAGEGGGRERGDRPDRGPRRERRGEPSNA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The presence of KH type-2 domains is critical for the interaction of the designed protein with its intended targets.2. The protein design should emphasize Basic and acidic residues compositional bias.3. For general function, the protein need meet that Binds the lower part of the 30S subunit head4. The designed protein should be involved in translation by localizing to ribonucleoprotein complex and exhibiting mRNA binding, structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "ARTEHYHEGTIPLQTLRADIDYGFAEANTTYGKIL",
    "response#2": "MTGLERKN",
    "response#3": "MLKINK"
  },
  {
    "reference": "MAVQPKETLQLESAAEVGFVRFFQGMPEKPTTTVRLFDRGDFYTAHGEDALLAAREVFKTQGVIKYMGPAGAKNLQSVVLSKMNFESFVKDLLLVRQYRVEVYKNRAGNKASKENDWYLAYKASPGNLSQFEDILFGNNDMSASIGVVGVKMSAVDGQRQVGVGYVDSIQRKLGLCEFPDNDQFSNLEALLIQIGPKECVLPGGETAGDMGKLRQIIQRGGILITERKKADFSTKDIYQDLNRLLKGKKGEQMNSAVLPEMENQVAVSSLSAVIKFLELLSDDSNFGQFELTTFDFSQYMKLDIAAVRALNLFQGSVEDTTGCQSLAALLNKCKTPQGQRLVNQWIKQPLMDKNRIEERLNLVEAFVEDAELRQTLQEDLLRRFPDLNRLAKKFQRQAANLQDCYRLYQGINQLPNVIQALEKHEGKHQKLLLAVFVTPLTDLRSDFSKFQEMIETTLDMDQVENHEFLVKPSFDPNLSELREIMNDLEKKMQSTLISAARDLGLDPGKQIKLDSSAQFGYYFRVTCKEEKVLRNNKNFSTVDIQKNGVKFTNSKLTSLNEEYTKNKTEYEEAQDAIVKEIVNISSGYVEPMQTLNDVLAQLDAVVSFAHVSNGAPVPYVRPAILEKGQGRIILKASRHACVEVQDEIAFIPNDVYFEKDKQMFHIITGPNMGGKSTYIRQTGVIVLMAQIGCFVPCESAEVSIVDCILARVGAGDSQLKGVSTFMAEMLETNFLMQLYEMTNKAKAHETCVCDQSFGIHVAELANFP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be involved in intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator, germ cell development, postreplication repair by localizing to MutSbeta complex, nucleus, MutSalpha complex, nucleoplasm, chromosome and exhibiting single guanine insertion binding, magnesium ion binding, ATP hydrolysis activity, MutLalpha complex binding, ATP binding.2. For general function, the protein need meet that Component of the post-replicative DNA mismatch repair system (MMR). The designed protein sequence is ",
    "response#1": "IATICGNDFMQNALHIDWKHNDLHLSGWVIQPQFARHQNDLNYCYINGRMVKDKVITHAIRQAYSEYLSNEKYPAFVLFIDLNPNEVDVNVHPTKHEVRFHQARLIHDFIYQGMTNAC",
    "response#2": "VIAIFGRDFLEPLIPVKWEGLSLSISGLISPPGVAKMNRKRQLIFVNQRPIRSPLLYRALDEGYRGLLLAREQPLVILQIAVPPDSIDVNVHPQKSEIRFRDEQSIFRSLCGLLRDTLN",
    "response#3": "LINVFGTKMSQSLDEFTASDSLFKVNGLISKIGIGSGTGQSISNSSSSSSQSSSQLSSSSSSSSSSQSSQLSIGSLSRSCADRQFFFVNSRPFEHSKLAKEINSLYQSFHKRGSYPVVIFNIEMPTNNYDVNVTPDKRTIFIQKEQQLLLLITDGLKTMWR"
  },
  {
    "reference": "SDDDGTVVLTTSGPIRGKRLQVGSAAVTAF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a specific function within the extracellular region.2. The protein must exhibit the following characteristics: Rathke gland secretions may function as pheromones, as predator repellents, or contribute to the maintenance of the turtle shell.. The designed protein sequence is ",
    "response#1": "IMGGSECYKSKHPFLVYLYNSAGFFCSGTLLNHEWVLTAAHCNRDDIQLKLGVHNVHVHYEDEQIRVPKEKLCCLSTKNCTQWSQDIMLIRLNSSVNNSKHIEPLSLPSRPPSMGSDCTVMGWGTITSPKVTYPKVPHCVDIKILHNPVCQAAYPTMSRKNILCAGVLEGGKDSCKGDSGGPLICDGQIQGIVSWGRFPCAQLLEPGVYTKVFDYIDWIRGIIR",
    "response#2": "IMGGSECYKSKHPFLVYLYNSAGFFCSGTLLNHEWVLTAAHCNRDDIQLKLGVHNVHVHYEDEQIRVPKEKLCCLSTKNCTQWSQDIMLIRLNSSVNNSKHIEPLSLPSRPPSMGSDCTVMGWGTITSPKVTYPKVPHCVDIKILHNPVCQAAYPTMSRKNILCAGVLEGGKDSCKGDSGGPLICDGQIQGIVSWGRFPCAQLLEPGVYTKVFDYIDWIRGIIR",
    "response#3": "IMGGSECYKSKHPFLVYLYNSAGFFCSGTLLNHEWVLTAAHCNRDDIQLKLGVHNVHVHYEDEQIRVPKEKLCCLSTKNCTQWSQDIMLIRLNSSVNNSKHIEPLSLPSRPPSMGSDCTVMGWGTITSPKVTYPKVPHCVDIKILHNPVCQAAYPTMSRKNILCAGVLEGGKDSCKGDSGGPLICDGQIQGIVSWGRFPCAQLLEPGVYTKVFDYIDWIRGIIR"
  },
  {
    "reference": "MIVTMKVMEKKNKKSWILYIVMGLITPCLSSSQLYATVYAGVPVWEDAKPVLFCASDANLTSTEQHNIWAAQACVPTDPTPHEYPLENVTDRFDIWKNYMVEQMQEDIISLWEQSLKPCVQMTFMCVQMNCTDRNTTANNTAAENLMKQCEFNVTTIVKDKKEKKQALFYVADLVKLNETGNGTGNMYTLINCNSTTITQACPKVSFEPIPIHYCAPAGYAIFKCNSAEFNGTGTCKNITVVTCTHGIKPTISTHLILNGTLSKEKIRIMGRNISDSGKNILVTLNSSVNITCERPYNQSVQQINIGPLAWYSMGIRESNGSNSRSAFCTFNTTDWVNTLKQTAERYLELVNNTGNNNVSMIFTHSTGGDPEVTFLHFNCHGEFFYCNTSGMFNYTFTCTGTTCSSQNSTKNDSTIPCKIKQVVRSWMKGGSGLYAPPISGQLTCSSNITGMILQMERPWNNSNNSATFRPEGGNMEDIWRTQLFNYKVVRVKPFSVAPTKIARPVIGTGTHKEKRAVGLGMLFLGVLSAAGSTMGAAATALTVQTHTLMKGIVQQQDNLLRAIQAQHQLLKLSVWGIRQLRARLLALETFIQNQQLLNLWGCKGNLICYTSVKWNDTWKGNSTTSLENIWENLTWQEWDQQISNISSTIYDEIQKAQVQQEQNEKKLLELSEWASIWSWFDITKWLWYIKIAIIIIGALVGVRIVMIVLNLVRNIRQGYQPLSLQIPNHHQSEVGTPGGTGEGGGEGGRPRLIPLQQGFLQLLYTDL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should contain one or more Human immunodeficiency virus 1 envelope glycoprotein Gp120, Retroviral envelope protein GP41-like domains that are essential for its function.2. The protein's functional design necessitates the inclusion of a YXXL motif; contains endocytosis signal, Di-leucine internalization motif motif.3. The designed protein should contain Cytoplasmic topological domains.4. For general function, the protein need meet that The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; Transmembrane protein gp41: Acts as a class I viral fusion protein5. The protein should be designed to localize specifically to viral envelope, host cell plasma membrane, host cell endosome membrane in order to perform its role in fusion of virus membrane with host plasma membrane, fusion of virus membrane with host endosome membrane more effectively. The designed protein sequence is ",
    "response#1": "MRVKGTQMNWPHLWRWGTLILGLVIICSASNNLWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQEMVLENVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCSNYNNSTIAGGDMKGEIKNCSFNITTELRDKRQNIYSLFYRPDVVQIDGENSGHYRLINCNTSTMTQACPKVSFEPIPIHYCAPAGFAILKCNNKTFNGKGLCKDVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENLTDNTKTIIVHLNQSNPVKIVCTRLNNNTRRSIRIGPGQTFFANDIIGDIRQASCKIEGNWTETLSAVAGKLKEHFTNAKIIEFKPASGGDLEITTHTFNCGGEFFYCDTSNLFNSNWTVTDSEPKLNSTEPNDNITLQCRIKQIIRMWQRVGQAMYAPPIPGIIECKSNITGLILTRDGGPPNTTETFRPGGGDMRDNWRSELYKYKVVKIEPIGVAPTGARRRVVGREKRAVGIGAMIFGFLGAAGSTMGAASVTLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKSLDQIWDNMTWMEWEREISNYTSLIYTLIEESQNQQEKNEQELLALDKWASLWNWFDISNWLWYIKIFIMIVGGLVGLRIVFSVLSIVNRVRQGYSPLSFQTHIPLQVRGPDRPEGIEGEGGERDRNRS",
    "response#2": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLSDQKFLGLWGCSGKIICTTNVPWNSTWSNKSFDQIWXNMTWTEWEREISNYTDHIYEILTESQNQQDRNEKELLELDKWASLWNWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTPSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSTIAGGDMKGEIKNCSFNITTELRDKRQNIYSLFYRPDVVQIDGENSGHYRLINCNTSTMTQACPKVSFEPIPIHYCAPAGFAILKCNNKTFNGKGLCKDVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENLTDNTKTIIVHLNQSNPVKIVCTRLNNNTRRSIRIGPGQTFFANDIIGDIRQASCKIEGNWTETLSAVAGKLKEHFTNAKIIEFKPASGGDLEITTHTFNCGGEFFYCDTSNLFNSNWTVTDSEPKLNSTEPNDNITLQCRIKQIIRMWQRVGQAMYAPPIPGIIECKSNITGLILTRDGGPPNTTETFRPGGGDMRDNWRSELYKYKVVKIEPIGVAPTGARRRVVGREKRXXXXGNLWVTVYYGVPVWKEAEATLFCASDAKAQKAEAHNIWASQACVPTDPDPKAIELSNVTENFNMWTNAMVNQMQQDVISLWDQSLKPCIKLTPLCVTLHCSIPKFDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLANVTENFNMWKNNMVEQMHEDVISLWDQSLKPCVKLTPLCVTLNCTKLKDELNATASNSSTAANNSSEMNITNTSDMKNCSFKITTSIRDKVQKEYALFYKTDIVPINSKNNSYSYRLISCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNNKTFDGKGPCINVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSDNFSNNAKTIIVQLKQPVEIDCIRPYNNTRKSIHIGPGRAFYTTGEIIGNIRQAHCNISKANWTNTLRQVAKKLREQFGNKTIIFNQSSGGDLEIVTHSFNCGGEFFYCNTSPLFNSTWNSTTNGNETEGSNNGTITLQCKIKQIINMWQEVGKAMYAPPIRGQIRCISNITGLLITRDGGNNETDSTESFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREK",
    "response#3": "MRVKETQRNWPNLWKWGTLILGLVIICSASDNLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPADPNPQELELKNVTENFNMWKNDMVNQMHEDVISLWDQSLKPCVKLTPLCVTLNCSNVSSNSSGTSITDIKNCSFNMTTVLKDKKEKVHALFYRLDIVPINDNDNDSHSNSSSMYRLINCNTSAITQACPKVSFDPIPIHYCAPAGFAILKCKDENFNGSELCQNVSTVQCTHGIRPVVSTQLLLNGSLAKEGVKVRSENFTDNAKNIIVQLAQPVRINCTRPNNNTRKSIRIGPGQSFYATGEVIGNIRQAHCNISRTEWNKTLHQIAKQLRKHFSNKTIIFNPSSGGDLEITMHSFNCRGEFFYCNTTKLFNSTWLNSTWNDTTQANNTNENITLPCRIKQIVNMWQGVGQAMYAPPIAGMIQCVSNITGLILTRDGGDNSSNNETFRPGGGNMKDNWRSELYKYKVVQIEPLGVAPNKAKRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASLTLTVQARLLLSGIVQQQNNMLRAIEAQQHLLQLTVWGIKQLQARLLAVERYLRDQQLLGIWGCSGKLICTTAVPWNASWSNKSLNDIWNNMTWMEWEKEINNYTNLIYNLLEESQNQQEKNEQELLELDKWASLWNWFDITKWLWYIKIFIMIVGGLIGLRIVFAVFSIVNRVRQGYSPLSLQTRFPAQRGPDRPEGIEEEGGE"
  },
  {
    "reference": "MFWLEQFERYIDHTGERVDVNYPFIQVKTDSRKVSSETLFVALPGVSSNGWDYLSLVAEKGCRYAVVPTYVAKGLIASNSTDLNIIGSDDVYALLSSIVKKTIGSFPSHLIGVTGTNGKSSVSYYIAQLADAMGLSGAIIGTFGVGKLNNLESAKQTTPDVLTMLKTLAQFNQDGVDLVAFEASSHALDQDRVAGIEIDTAVFTNLTQDHLDYHGTMESYATAKRKLFRLDTLKHAVFCSKSDYCNFMAEDSKGDKSYYGERPSDDFVIRDVTYHSLGCSFLLLTKSGHYSVELPLMGEFNVDNAVAALASLWPLFDDKVALISSLTSLKGAPGRMERVTLSEGANVPVVLVDYAHTPDALSVALKALAVHSKGPITCVFGCGGDRDKGKRPEMMRAALDLADKVVLTTDNPRSEIPSEIMQDALSGVAECERQRVILDEDRRSAIEFAIATASINEIVLVAGKGHETYQEIGGVKSHFDDREEALNALKKYAQRT",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design must be focused on enhancing the efficiency of cell wall organization. The designed protein sequence is ",
    "response#1": "ITGTNGKTSVATFCRQIFSRLGHTSASVGTLGVVTQDAAGVENAITGPGLTTPDAADLAKHMADMAADGVTHLTLEASSHGIDQRRLDGVSLVAAGFTNLTQDHLDYHATMEAYRTAKLRLFEQLLPRGRTAVLNADSDAYNAFAAMSIMSGLNVMSVGERSSHLCMTGRDLTVDGQILYLEYLGKKYETKLPLAGLFQASNALVAAGLT",
    "response#2": "VRGGNTLKGTIPISGAKNACLPELAATLLTEDTVTLRNVPHLRDVTTMLELLGQHGAAITIDEKLGVSIDCKSIQNTMAPYDLVRTMRASVLVMGPLVARCGHAEISLPGGCAIGSRPINLHLRGLEMMGAHVTLEDGYVRIKAGRLKGAHIVFDLVTVTGTENLLMAATLADGITILDNAAAEPEVVDLANLLMAMGAKIDGAGTRTITIEGVKNLHGTSHDILPDRIETGTFMVAAAVTGGDITMTGTYPALLEAHIAKMREAGCQIDEMDRAIRVRAEAGTLRAVDITTLPHPGFPTDLQAQMMVLLTVAKGAAQIKETIFENRFMHVSELQRMGADITVQGNTAIVRGVPQLRGAPVMATDLRASASLVLAGLCAEGETLISRVYHIDRGYERIEEKLGLQ",
    "response#3": "ITGTNGKTSVATFCRQIFSRLGHTSASVGTLGVVTQDAAGVENAITGPGLTTPDAADLAKHMADMAADGVTHLTLEASSHGIDQRRLDGVSLVAAGFTNLTQDHLDYHATMEAYRTAKLRLFEQLLPRGRTAVLNADSDAYNAFAAMSIMSGLNVMSVGERSSHLCMTGRDLTVDGQILYLEYLGKKYETKLPLAGLFQASNALVAAGLT"
  },
  {
    "reference": "MVTSAGVGHAEYNNGADVQHADYAHLTSVGQVEQKPLGGRLGALAEYYKPNVASMEEYRAMHAQSITDPAAFYGERARTYVDWFRPFDAVFLPGPDGRPSFDNNAWFVNGQLNACYNLVDRHAARTPDKVAIIYEADEPGEGYSLTYRELLAQVCKVAQVLQYSMGVRKGDTVAVYMPMIPQALVTLLAISRIGAIHSVVFAGFSSNSLRDRINDARSEVVVTTDESKRGGKIIETKRIVDDAIKETPQLRKVLVYKRTCNPSVSYVADRDLDWDTEVKKYKSYCPCEPVDSEHPLFLLYTSGSTGAPKGVQHSTAGYLLQAYLSMLYSFDVHSDDIFFTAGDIGWITGHTYVVYGPLFSGCTTVVFEGTPAYPSYSRYWDIIDKYSVTQFYVAPTALRLLKRAGDSYVDGYSLRTLRSLGTVGEPIAAEVWEWYYTVIGKREIPVIDTYWQTESGAHLVTPLAGGSTPMKPGSASFPFFGIDLAILDPQTGEELLGPNVEGVLAVKQPWPSFTRTIWNNHDRYLDTYLNPYKGYYFAGDGAARDSQGFIWILGRVDDVVNVSGHRLSTAEVEAAIIQESMVAECAVVGFADELTGQAIAAFVVLKQKSSWNTASERELQEIKKHLILSVRRDIGPFAAPKLIVLVDDLPKTRSGKIMRRILRKILAGEADQLGDVSTLSNPGIVKHLIESVKF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have AMP binding within endoplasmic reticulum, cytosol to regulate acetyl-CoA biosynthetic process from acetate.2. The ATP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "RELSKEEAYVQGLDAKTGASLKLTILNAEGRVWNLVAGGGASVVYADAVAVNGAADELANYGEYSGAPTDGQTYEYAKTVLDLMTRGEPRADGKVLFIGGGIANFTSPAVTFRAIARALGDYKDKLHAHKVSIWVRRAGPNYQEGLRVIREAGKKFDLPLKVYGPECHISGIVPMGLV",
    "response#2": "RELSKEEAYVQGLDAKTGASLKLTILNAEGRVWNLVAGGGASVVYADAVAVNGAADELANYGEYSGAPTDGQTYEYAKTVLDLMTRGEPRADGKVLFIGGGIANFTSPAVTFRAIARALGDYKDKLHAHKVSIWVRRAGPNYQEGLRVIREAGKKFDLPLKVYGPECHISGIVPMGLV",
    "response#3": "RELSKEEAYVQGLDAKTGASLKLTILNAEGRVWNLVAGGGASVVYADAVAVNGAADELANYGEYSGAPTDGQTYEYAKTVLDLMTRGEPRADGKVLFIGGGIANFTSPAVTFRAIARALGDYKDKLHAHKVSIWVRRAGPNYQEGLRVIREAGKKFDLPLKVYGPECHISGIVPMGLV"
  },
  {
    "reference": "MAEELRRFLYKKLTSVEGLHAIVVSDRDGVPVIKVANENAPEQALRPAFLSTFALATDQGSKLGLSKNKSIICYYSTYQVVQFNQLPLVVSFIASSNANTGLILSLEKELGSLFKELRQAVIS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be optimized for stability and expression in late endosome membrane where it will participate in protein localization, cellular response to amino acid stimulus.2. The protein must exhibit the following characteristics: Adapter protein that may regulate the MAP kinase cascade.; As part of the Ragulator complex, may activate the TOR signaling cascade in response to amino acids. The designed protein sequence is ",
    "response#1": "MAPPKALETIGKTLHSQYERWQPKARYKLQLDPTLEEVKKLCNTCRKFARTERVLFHYNGHGVPKPTANGEIWVFNKSYTQYIPLPITDLDSWLKTPSIYVFDCSAAGMIVKAFE",
    "response#2": "MAPPKALETIGKTLHSQYERWQPKARYKLQLDPTLEEVKKLCNTCRKFARTERVLFHYNGHGVPKPTANGEIWVFNKSYTQYIPLPITDLDSWLKTPSIYVFDCSAAGMIVKAFF",
    "response#3": "MAPPKALETIGKTLHSQYERWQPKARYKLQLDPTLEEVKKLCNTCRKFARTERVLFHYNGHGVPKPTANGEIWVFNKSYTQYIPLPITDLDSWLKTPSIYVFDCSAAGMIVKAFR"
  },
  {
    "reference": "MALEVIVLAAGQGSRMRSRFPKVLQKLAGRPLLQHVLDTAAALAPSRIHVVVGHGADAVREAFADRGLNWVDQSERLGTGHAVLQAMPAVDTAATVLVLYGDVPLVAEDTLRSLRAMAASGPALLTASLDNPAGYGRILRDANGQFSAVVEDRDASPEQRALREVNTGILAAPAAQLAQLLPLVGNDNQQGEYYLPDILTLALERNIAVHTAGVGHLLEVQGVNDPFQLAQLERALQLQQAEALMQAGARLADPARIDVRGALRCGRDVSIDVNCVFIGEVELADGVSVGPNCVLEDCRVGADSQVLAMSHLEGAEVGAGCSVGPFARLRPGTRMADSARVGNFVETKKTRIGTGSKVNHLTYLGDCDVGEEANIGAGTITCNYDGVNKHPTRIGDGAFVGSNATLVAPLEIGDNGFVAAGSTITRNVSSDELAVGRGKQRNIPGWKRPGSDKEG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc)2. The protein should have a high affinity for acetyl-CoA, Mg(2+), UDP-N-acetyl-alpha-D-glucosamine.3. The designed protein should be involved in cell wall organization, peptidoglycan biosynthetic process by localizing to cytoplasm and exhibiting magnesium ion binding, glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity.4. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "IILAAGKGTRMQSDLPKVLHHVAGAPLLIHAMRSAAALEPEKTVVVTGHGADLVEAAARGWSDAVEVVLQAEQLGTGHAVLQAKDALSGFDGDAIVLYGDTPFIRPETLEAMIDARAAHDIVVLGFEAADPTGYGRILRTQDGEVLRIVEQKDATDAERAVRLCNSGIMAFDGKRLFDALARVGNANAKGEYYLTDIVEIARRDGRRAGVVVCDEAETLGVNSRGELARAEALWQARRREALMAAGVTLIDPDHTYIEADVEIGMDTIIYPGTILKGTTRIGADCMIGPSADLTDMHVEDGAEIRYSVLEGSTVSRTAKVGPYAYVRPGSSLGENSKVGCFVDVKKSTLGQGSKVSHLGYIGDSEVGQNVNIGCGAVTVNYDGQKKHQTVIEDGAFVGCNVNMVAPVTIGKGAYVAAGSTVTEDVPENSLAIARQRGPVHLGRDVEIGPYTVLESTVAEDVWIGPFAHLRPGADIGARCRIGNFAEVRDSRLGRGVKVKHLSYLGDAEVGDDSNIGAGTITCNYDGKAKHRTVIEDGAFVGCNVNMVAPVTIGKGAYVAAGSTVPADAVVVLAAGEGKRMRSATPKVLHAIGGRSLLGHAVHAVAALEPEHLVVVVGHGRDQVEPAVAAMADALARPVVTAVQEQQNGTGDAVSCGLAALPADLSGTVLVTYGDVPLLVPEALRALHEAHGG",
    "response#2": "VVIMAAGKGTRMKSKLPKVLHLLAGRALLQHVVDTAAQLSARQVVVITGHGAMEVEAAVSGNTGASAGFDINFVRQEPQLGTGHAVQQAVPLLRDDGMVVVLSGDVPLIQAATLRHLIAAAGTDGLGLLTVAMDDPTGYGRIVRKGGQVLAIVEQKDATPAQRQIREVNTGILAAPSARLAAWLGRIGNDNAQQEYYLTDVIDMAVADGVPVATAQPASVAEVEGANNRVQLAALERAHQRAEAERLMLAGVTIVDPATTRIDAGVEIGQDARIEPWTILEGATAIASDAIIGPNTHVRDSRIGPRTRVWSSVVEESTVAEDVWIGPFAHLRPGADIGARCRIGNFAEVKRSRLGAGTQQHHFSYLGDAEIGESVNIGAGSVTANFDGAAKHRTEIGNGAKIGVDTMMVAPVTIGENAVTGAGAVVTRDVAPGKTVVGWPRPTKK",
    "response#3": "VVIMAAGKGTRMKSKLPKVLHLLAGRALLQHVVDTAAQLSARQVVVITGHGAMEVEAAVSGNTGASAGFDINFVRQEPQLGTGHAVQQAVPLLRDDGMVVVLSGDVPLIQAATLRHLIAAAGTDERLGLLTTRLADPTGYGRIVRDASGTVRAIVEHKDASEAQRAIDEVYTGIMAAPTARLKAWVSALENDNAQGEYYLTDIVAQAVADGVPVVATVAEDAAEVLGVNSPLQLAELERAHQLRQARRLMEALAAAEAIWQQRARAEAMAEGVTMVAPETVWLAYDTAFGRDVVVEPNVFFGPGVTIEDDVHIRANCYLEGIDGKSRAGLRIAAGAKVGPFARLRPGTAIGRDVHIGNFVEVKNATMEDGSKANHLSYVGDARVGAGANIGAGTIFCNYDGFNKNLCDVGAGVFIGSNSALVAPVKIGEGAYVAAGSTITRDVAPDSLAIARAARAELATAEATFQARARAEAMANGVTLHAPETVYFSHDTFIGADTVIEPNVVFAPGVTIENGAQIRAFSHLEGCHVSRGGVVGPYARLRPGTELAEDVKIGNFVEVKNAVLDTGAKVNHLSYIGDAQVGARSNIGAGTITCNYDGVFKHRTTIGRDVFVGSNTMLVAPVTLGDESMTATGTIVTRDVPEGDMAVARARSRLPKVLHPLAGRPLIHHVLDALRDAGIERQVVVVGHGADAVEAAIAGRAIAVLQEPQRGTADAVRVALPSVASAKTLVVTMGDVPLQTAELYAMLLREHTTGDAVLTLVSAQLADPTGKLARWQRPVKKKAQ"
  },
  {
    "reference": "MAKVSMIQREAKRTKLVAKFAEKRAALKAIISDVNASDDEKWEATLKLQALPRDSSSSRQRNRCQITGRPHGVYRRFGLSRIKLREAAMRGDVPGLKKASW",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that can perform structural constituent of ribosome, rRNA binding in ribonucleoprotein complex, cytoplasm, ribosome to support translation.2. The protein must exhibit the following characteristics: Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site.. The designed protein sequence is ",
    "response#1": "MAPGACMPLGKVPLGTTVHNVELKPGAGGQLVRSAGSSARVIARDGNYTLVTLPSGEKRLILSTCHATIGAVSNADRKNTKLGKAGRSRWLGRRPSVRGVAMNPVDHPMGGGEGKTSGGRHPVSPWGFN",
    "response#2": "MAPGACMPLGKVPLGTTVHNVELKPGAGGQLVRSAGSSARVIARDGNYTLVTLPSGEKRLILSTCHATIGAVSNADRKNTKLGKAGRSRWLGRRPSVRGVAMNPVDHPMGGGEGKTSGGRHPVSPWGFR",
    "response#3": "MAPGACMPLGKVPLGTTVHNVELKPGAGGQLVRSAGSSARVIARDGNYTLVTLPSGEKRLILSTCHATIGAVSNADRKNTKLGKAGRSRWLGRRPSVRGVAMNPVDHPMGGGEGKTSGGRHPVSPWGFR"
  },
  {
    "reference": "MAQRKIALACSVCGSRNYTITASATRTKRLEVMKFCKFCGKHTLHRETR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must be able to perform structural constituent of ribosome under specific conditions. The designed protein sequence is ",
    "response#1": "PDVKEVKMRYKIDEHDYQVRINQAKRFLKAGDKVKATVNFRGREIQHAHLAKELLDRMATDLATEADIQQAPKREGRNMMMFLSPG",
    "response#2": "GFEEKVVTIRRVTKVTKGGRHFRFAAVVVVGDKKGQVGLGTGKANEVPEAIKKAVKEAKKNLIRVK",
    "response#3": "DHHHHHHPEGL"
  },
  {
    "reference": "MKAIKNKGQDKTMNETTHRFARLVDLSAVQATSTEADVRACAELAARYNIISVHVLPCWTRFLSTLLPQQGTGEVMIGGPVGFPGGGHTTDTKVQEVRQLIADGAREVDMVVNIGKVLSGDYDYVREDLRRVVEAAAPVPAKVILETHYLNEEQIRRVCEIAVEVGMKWVKTSTGWAPTGATVEKVRIIADQLKGRIDIKGAGGIRDLATVRALYQLGVRRFGMSHGAVTKVLAELEQHPERFPELNAD",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein should be able to manipulate the activity of enzymes in the Carbohydrate degradation, 2-deoxy-D-ribose 1-phosphate degradation, D-glyceraldehyde 3-phosphate and acetaldehyde from 2- deoxy-alpha-D-ribose 1-phosphate: step 2/2 pathway, either by activating or inhibiting their function.2. The protein must exhibit the following characteristics: Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5- phosphate..3. A key design requirement for the protein is the optimization of the Schiff-base intermediate with acetaldehyde, Proton donor/acceptor to achieve high catalytic efficiency for 2-deoxy-D-ribose 5-phosphate = acetaldehyde + D-glyceraldehyde   3-phosphate.4. The designed protein should have deoxyribose-phosphate aldolase activity within cytoplasm to regulate carbohydrate catabolic process, deoxyribonucleotide catabolic process, deoxyribose phosphate catabolic process. The designed protein sequence is ",
    "response#1": "MSLENQVNDIEQAIIDIKNGKMVIVVDDEDRENEGDLTIAAQFVTPEAINFMATHGRGLICLSMTEARLDQLNLPLMVSNNTESMKTAFTVSIDAKEDITTGISASDRSDTIKAAIDPKTQAKDLGRPGHIFPLRAKEGGVLRRAGHTEAAVDLARLAGLYPAGVICEIMNEDGTMARVPDLMQIADKFNLKIISIKDLIAYRLKSESLIQREIVLSTDV",
    "response#2": "MXSRVXXIXNXKXXRLXXXGEXXEXXRXYXXAXGXI",
    "response#3": "MKVLKDILYGASIEAITGSTSIAXTXKMVXEXVXNXGXKAKIXIXGXN"
  },
  {
    "reference": "MLLSHSRSPYKPRYVIKSKTSMIKKCKMKKLLWSWIILFNIHVNGMMMEFDEMEWFTVFNGTKVFQTQNDVFSEAKFPMVGLTLIQSAAAKGAVCLDGSLPGYHLHRGFGSGANNWLVQLEGGGWCDTIRNCVYRKTTRRGSSSYMEKEIPFTGILSDKAADNPDFYNWNRVKVRYCDGGSFSGDSENKAAQLQFRGKRIWLAAMEDLMAKGMRQAKQALLSGCSAGGLAVILRCDDFGKLFPPSTRVKCLSDAGFFLDAIDVSGGRSLRRLYAGVVRLQNLQTNLPQYCVNRLNPTSCFFPQNLINQVKTPLFILNAAYDSWQIQESLAPKSADPSGSWNDCRLNYAKCSASQIQFLQGFRTRMVNLVKGFAMPSKNGVFLNSCFAHCQTERHDTWFAQNSPAIKNKGIAVAVGDWYFERGGAKLIDCAYPCDKTCHNLVFRR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Hydrolyzes acetyl esters in homogalacturonan regions of pectin The designed protein sequence is ",
    "response#1": "LPRFNASDFLERSRNGRIVFVGDSIGRNQWESLLCMLSQAVSNKSEIYEVNGNPISKHKGFLSMRFPEQNLTVEYHRTPFLVVVGRPPENSPVDVKMTVRVDEFNWQSKKWVGSDVLVFNTGHWWNEDKTFIAGCYFQEGGKLNKTMGVMEGFEKSLKTWKSWVLERLDSERSHVFFRSFSPVHYRNGTWNLGGLCDADTEPETDMKKMEPDPIHNNYISQAIQEMRYEHSKVKFLNITYLTEFRKDAHPSRYREPGTPEDAPQDCSHWCLPGVPDTWNEILYC",
    "response#2": "LPRFNASDFLERSRNGRIVFVGDSIGRNQWESLLCMLSQAVSNKSEIYEVNGNPISKHKGFLSMRFPEQNLTVEYHRTPFLVVVGRPPENSPVDVKMTVRVDEFNWQSKKWVGSDVLVFNTGHWWNEDKTFIAGCYFQEGGKLNKTMGVMEGFEKSLKTWKSWVLERLDSERSHVFFRSFSPVHYRNGTWNLGGLCDADTEPETDMKKMEPDPIHNNYISQAIQEMRYEHSKVKFLNITYLTEFRKDAHPSRYREPGTPEDAPQDCSHWCLPGVPDTWNEILYC",
    "response#3": "LPRFNASDFLERSRNGRIVFVGDSIGRNQWESLLCMLSQAVSNKSEIYEVNGNPISKHKGFLSMRFPEQNLTVEYHRTPFLVVVGRPPENSPVDVKMTVRVDEFNWQSKKWVGSDVLVFNTGHWWNEDKTFIAGCYFQEGGKLNKTMGVMEGFEKSLKTWKSWVLERLDSERSHVFFRSFSPVHYRNGTWNLGGLCDADTEPETDMKKMEPDPIHNNYISQAIQEMRYEHSKVKFLNITYLTEFRKDAHPSRYREPGTPEDAPQDCSHWCLPGVPDTWNEILYCK"
  },
  {
    "reference": "MTVNLLDFDGESLTAWFAEQGEKPFRAKQVLRWIHRSGVADFDAMTDIAKSLREKLKAKAVVAPPAVVSDKLSDDGTRKFLIDVGNGNAVETVFIPEDDRGTLCISTQAGCALDCAFCSTGKQGFNRNLSVAEIIGQLWQANHALGAVHGDERVISNVVLMGMGEPLANFENSVAALKLMLDDNAYGLSRRRITVSTSGLVPVMDRLGDECPVALAVSLHAPNDKLRDQLVPINQKYPLKELMAACQRYLEKAPRDFITFEYIMLDGINDTDAHARELLALVKSVHCKFNLIPFNPFPGSPFRRSPAERVRHFADILMQAGIVTTTRKTRGDDIDAACGQLAGQVQDKTKRTTGRVIPLKEARS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be able to bind [4Fe-4S] cluster, S-adenosyl-L-methionine ligand in a variety of conditions2. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs; m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.3. The designed protein is the optimization of the S-methylcysteine intermediate, Proton acceptor to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.4. The protein contains novel Radical SAM core domains that confer a unique function or activity.5. The protein design should incorporate a binding pocket that specifically recognizes [4Fe-4S] cluster to enhance enzymatic efficiency.6. A protein that can perform tRNA binding, metal ion binding, rRNA (adenine-C2-)-methyltransferase activity, rRNA binding in cytoplasm to support rRNA base methylation. The designed protein sequence is ",
    "response#1": "KKPPPHLADLDPAGRKALLEDNGLPGFRAKQLSTHYFARLVDAPEQMTDLPAAQRGELVSTLLPELMTPVRTSEADRGTTRKTLWRLFDGALVESVLMRYTDRTTMCVSSQAGCGMACPFCATGQGGLQRNMSTAEIVEQVMAGARSLARGEVPGGPGRVNNVVFMGMGEPLANYKAVVGAVRRLTDPAPDGLGMSARGVTVSTVGLVPRIRQLTEEGVPLTLALSLHAPDDELRNELVPINTRFSVAETVDAAWQYARTTKRRVSIEYAMMRGINDQGWRADLLADVLYATGADWGWVHVNLIPLNPTPGSKWTASDPADEQEFVRRLEARGIPTTVRDTRGREIDGACGQLAASAHARAGA",
    "response#2": "NGKVNLMGMSASSLADLMEMMGEKRFRATQILKWIHQMGVRDFDQMTNLSKVLRGKLSEGMEIVVPEVVSCENSSDGTRKWLVQVENDSCIEMVFIPEGSRGTLCVSSQIGCALDCSFCATGKQGFNRDLTASEIIGQLWIAADSFNQFSSTTTRRITNVVMMGMGEPLMNFDNVVEAMSIMLDDHAYSLSKRRVTLSTAGVLPALDRLGDVLDVSLAISLHAPNDELRNKLVPLNKKYSINEFIDSARRYLSKMPDSRRKVTVEYTLMDQVNDRASHALELSSVLRELPCKINLIPFNPFPGTEFRRVSDVAMNRFRDILHREGYTVTVRTTRGDDIAAACGQLAGLVNDKTRRQRP",
    "response#3": "EEGRGTLCVSSQVGCTLTCSFCHTGTQKLVRNLTSGEILSQLILARHRLGDFPDVEAAPGTMVPSEGRKVSNIVMMGMGEPLYNFENVKQALLIASDGEGLSISRRRITLSTSGVVPEIRRAGEEIGVMLAISLHAVRDDLRDELVPINKKYPLKDLLQACRDYPGLSNARRITFEYVMLKGVNDSLEDARDLVKLLKGIPAKINLIPFNPWPGSAYECSDWEQIERFADFVNHAGYASPIRTPRGRDILAACGQLKSESERMAKRKQKEAVA"
  },
  {
    "reference": "MAPQKPAAAAATPSDKPESAPTADADVSAPEVLPNNELTGDMLYRITRAELEFKQGEWQGPFVTMMALAQQTRDPRLAQRATEIAMVAKQGSEAMAASRLWRSLAPDSDEAAQYFLGFAVLGNDLAEVEPIFARRLQAVQGPARAVAMFQMQQLLQRAQDKAAAFALLERVLAPYDAMAESHLILAQGAFAVNDTARARTEADRAAALKPDSELAVMTQAQVSGDIGIALKRLNDFLDNYPKAREVRGAYARVLIDAKQPEAARQQFLVLLKDQPDNLATLYALGVMAMQGNDLAGAETYFTRFVTVLDEIPNDERDVTKVLLMLTQLAEQRGDLDTAIKWLDKVDRSDEKLYLAARLKWAVLTARQGRLDAARKELSELTANGADEQAQIFQTDAQVLRDAGDTTTAYMVLQNAVKRYPDNPDLLYDFALSAEKVGKTDLMEQALRQVMIAAPDNQHAYNALGYSLAERNERLPEAQALVEQALKMAPGDPFIMDSMGWVQFRMGNLQEAESTLRRAYAVRPDPEIAVHLGEVLFASGDVDGARKLCAVATGRLRLRSPFDGRRGRLSGQHRIERPHRRQLQPRRQEGSAQRQLQLGAKPVPHRCQPDFAHRPDPGPDQRHARVRHAAAKRPGAAHRARYRQPDAPDPGLDPAPWSRATAGRSITWAGTMTRPPCRNRAASTSPAWRWASRWTTCRSASCSTPPRLHHEPVAELPGAGQAQSVFARQRPPRRRLPPAANRVPTGGPRRHAALHAARRHGAAARDGAR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be designed to have high stability and activity towards ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-   diphosphate + AMP + H(+).2. The protein design should consider the endogenous proteins and enzymes present in the Pyrimidine metabolism, CTP biosynthesis via salvage pathway pathway to avoid interference with native metabolic processes.3. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining ATP hydrolysis activity. The designed protein sequence is ",
    "response#1": "SPVLVDYLQTRDLGEVVVVSPDVGGVARARAFAKQMHDAPLAIIDKRRAAHNVAESLTVIGDVAGKTAILIDDMIDTGGTICQGARLLRRNGAARVLCCATHAVFSPPATERLSEPGLFEEIVVTNR",
    "response#2": "SPVLVDYLQTRDLGEVVVVSPDVGGVARARAFAKQMHDAPLAIIDKRRAAHNVAESLTVIGDVAGKTAILIDDMIDTGGTICQGARLLRRNGAARVLCCATHAVFSPPATERLSEPGLFEEIVVTNG",
    "response#3": "VPPHPLIKHWLAVARDAATPSVLFRSAITELGRWLTYEAAREWLPTEETTVQTPLEICPATVINPQVPVAVVPILRAGLGLLEGAQTVLPLASIYHLGLVRNEETLEASCYLNKLPEKFDPQTRVLITDPMLATGGSIMKAMSELTERGVDPSLVRIVSVVAAPPALQKLNAAYSGLIVYTATIDETVNDQGFIVPGLGDAGDRIFGIRD"
  },
  {
    "reference": "MATLTQKQNAPKKVFAFRWSGVNRKGQKVSGELQADSINTVKAELRKQGVNVTKVSKKSQGLFSKGGAKIKPMDIAIVSRQITTMLSAGVPLVQSLQIIARSHEKASMRELMGQIAADVETGTPMSEALRRHPRHFDALYCDLVEAGEQSGALETIYDRIATYREKSEALKSKIKKAMFYPAMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPIFTQFVIGISRFMQHWWYVIFGGTAFAIFLYVRAWRASQKVKDNTDKFILTIPVVGMILHKAAMARFARTLSTTFSAGIPLVDALISAAGASGNYVYRTAVMAIRNEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVATIFEQEVDDLVDGLTSLLEPLIMVVLGVLVGGMVVAMYLPIFKLGDLVG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein localizes to the plasma membrane. The designed protein sequence is ",
    "response#1": "SVHKLRPGDIDVIAAMGDSLTAGAGIFADNEFNPNLIGFALGDSLTTHKESQLNIAESGAESADMMYMAEMLVKKIKNDPRINVQKHWKLISLMIGANDFCNEICSVSSPWSILENHKVDLLQVLRILRDNLPRTFVALIPPPHLKSLVDIRKEKSSFKCFVTTDVECSCLFGLAFQRYISIYYDIMRQWQILDMEIADYPEFQRNDFVVVAQPTLMNISIPLDSDGYSDMTYLSSDCFHVSQKTNARFANGLWNNLLETVH",
    "response#2": "SVHKLRPGDIDVIAAMGDSLTAGAGIFADNEFNPNLIGFALGDSLTTHKESQLNIAESGAESADMMYMAEMLVKKIKNDPRINVQKHWKLISLMIGANDFCNEICSVSSPWSILENHKVDLLQVLRILRDNLPRTFVALIPPPHLKSLVDIRKEKSSFKCFVTTDVECSCLFGLAFQRYISIYYDIMRQWQILDMEIADYPEFQRNDFVVVAQPTLMNISIPLDSDGYSDMTYLSSDCFHVSQKTNARFANGLWNNLLETVC",
    "response#3": "SVHKLRPGDIDVIAAMGDSLTAGAGIFADNEFNPNLIGFALGDSLTTHKESQLNIAESGAESADMMYMAEMLVKKIKNDPRINVQKHWKLISLMIGANDFCNEICSVSSPWSILENHKVDLLQVLRILRDNLPRTFVALIPPPHLKSLVDIRKEKSSFKCFVTTDVECSCLFGLAFQRYISIYYDIMRQWQILDMEIADYPEFQRNDFVVVAQPTLMNISIPLDSDGYSDMTYLSSDCFHVSQKTNARFANGLWNNLLETVCG"
  },
  {
    "reference": "MSKILKIIGREIIDSRGNPTIECEVLLEGGFVGLASVPSGASTGSFETWELRDQDKNRFMGKGVQKAVEIINNKIFYSLKNKNAIDQFDIDQTMINLDGTENKSNLGSNSILSVSLATAKAAASSKGMPLYQHIAEINNTPGVFSMPLPMINIINGGKHANNNIDIQEFMIQPISAKSITEAIRIGAEIFHSLGNLLKDKGMSTTVGDEGGYAPNFKSNEEALNVIQDAVHKTKYKLGKDITLAIDCAASELYNKTRKKYQFIGEGTEFSSQELTHYLKKLSNKYPIISIEDGQDESDWEGFLYQTKELGNSLQLVGDDLFVTNKNILKKGIKKGVANAILIKLNQIGTLTETIETIKIAKKFNYGVIISHRSGETEDTSIADLSVGTASGQIKTGSMSRSDRTSKYNQLIRIEEILNKKRAPFYGLKEVKSSF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have magnesium ion binding within cell surface, phosphopyruvate hydratase complex, extracellular region to regulate glycolytic process.2. The substrate binding site should be located in a region of the protein that is accessible to the ligand.3. A protein that is capable of catalyzing reactions within the glycolysis, Carbohydrate degradation pathway with high efficiency and specificity.4. Mg(2+) is required for the proper folding and stability of the protein.5. The designed protein must have a Proton acceptor, Proton donor that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "MLPLPCFNVINGGEHAGNKLAMQEFMICPTGATSFHEALRMAAETYHNLKLVIKKRYGMDATNVGDEGGFAPNIQANHEGLELIVEAIKQAGYTGKIEIGMDVAASSFWDAKESKYDLGFKVPADKKTPDMLVSGEGLIKLYEEWTSKYPIWSIEDPFDQDDWATYTRFTELIRNRIQIVGDDLLVTNPKRIVEARNKKACNALLLKLNQIGSVSEAVEACRLAREVNWGVMVSHRSGETEDAFIADLVVGLGCGQIKTGAPCRSERLAKYNQLLRIEEELGANAHYAAKM",
    "response#2": "MLPLPCFNVINGGEHAGNKLAMQEFMICPTGATSFHEALRMAAETYHNLKLVIKKRYGMDATNVGDEGGFAPNIQANHEGLELIVEAIKQAGYTGKIEIGMDVAASSFWDAKESKYDLGFKVPADKKTPDMLVSGEGLIKLYEEWTSKYPIWSIEDPFDQDDWATYTRFTELIRNRIQIVGDDLLVTNPKRIVEARNKKACNALLLKLNQIGSVSEAVEACRLAREVNWGVMVSHRSGETEDAFIADLVVGLGCGQIKTGAPCRSERLAKYNQLLRIEEELGANAHYAAKL",
    "response#3": "MLPLPCFNVINGGEHAGNKLAMQEFMICPTGATSFHEALRMAAETYHNLKLVIKKRYGMDATNVGDEGGFAPNIQANHEGLELIVEAIKQAGYTGKIEIGMDVAASSFWDAKESKYDLGFKVPADKKTPDMLVSGEGLIKLYEEWTSKYPIWSIEDPFDQDDWATYTRFTELIRNRIQIVGDDLLVTNPKRIVEARNKKACNALLLKLNQIGSVSEAVEACRLAREVNWGVMVSHRSGETEDAFIADLVVGLGCGQIKTGAPCRSERLAKYNQLLRIEEELGANAHYAAKY"
  },
  {
    "reference": "MPTNQLHLWLPERTQQLADRPLRYLQLDSRNVSRDDVFIALPGTREHGNQYINQALAQGAALVLTDSGSYQDERVLVISDLVAQMPALAARFYHQPQKQLQLVGVTGTNGKSSTCCYINQLAGQCATPAAVVGTLGYGHWQQLTPLLNTTPHYIDLQRILSELLQQKTELVAMEVSSHALVQQRVAGLQFQVAVFTNLSRDHLDYHGSMAEYGQAKSLLFQPDMSRCAVLNVSDPLGKALASRHDIPVWAYGKAEDCQGQPRFLGYHQLTAYEQGYRCLLSSHAGEHELHLPLLGEFNVQNALAAISSLLALGYDLTALLHAAGHLQPLAGRMEQFPFSHGVTVVVDYAHTPDALQQALQALRQHCGGRLWCVFGCGGDRDKGKRPIMGQLAQQYADHCIVTSDNPRSENPLQICQDIAAGMQSTDNYRLEPNRQLAIKLALVSAQPGDVILLAGKGHETTQIIGHEQQHYDERAYVRQLMSEMAS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding site should be stable and able to withstand changes in environmental conditions.2. A protein that can perform magnesium ion binding in cytoplasm to support cell cycle, peptidoglycan biosynthetic process. The designed protein sequence is ",
    "response#1": "VTGTNGKTSTAWWLAQALSKLGRRCGVVGTLGVGEPGAMVPTGLTTPDPVLLQQQLRRFVDEGFAACVMEASSIGIAEKRLDAVAVQVAMLTNFTQDHLDYHASMEAYWQAKRELFDWPGLKAAVINVDDAHGDQLAQSLAGRAMDLWTVSVTGPARLQARAVSYASGAMHFDVVEGGNSCSLTAPLVGQYNVSNLLGVIALLEAAG",
    "response#2": "AASVGVPVVPYQPLTLPTWKRTPELCMRRILETFTFPMFVKTAHLGSSIGVFEVHNETELKAKISEAFLYDTDVFIEESRLGSREIEVSCLGDACSCYYISEPHERRGSKGFIGYEEKYGFNGKSSATIQYDLNLSEESKTRVKELTERVYRVIQGKGSCRIDFFLDREGNFWLSEMNPIPGMTKSSPFLHDFARLGWTFEQIVHQLR",
    "response#3": "AASVGVPVVPYQPLTLPTWKRTPELCMRRILETFTFPMFVKTAHLGSSIGVFEVHNETELKAKISEAFLYDTDVFIEESRLGSREIEVSCLGDACSCYYISEPHERRGSKGFIGYEEKYGFNGKSSATIQYDLNLSEESKTRVKELTERVYRVIQGKGSCRIDFFLDREGNFWLSEMNPIPGMTKSSPFLHDFARLGWTFEQIVHQLR"
  },
  {
    "reference": "MTPDPHRPRILLGVSGGIAAYKAAELVRRLREREADVRVVLTDNAARFVTPLTFQALSGQPVRSSLWDDAAEAAMGHIELARWATRVVIAPASADLIARLAHGLADDLLTTLCLATEAPITIAPAMNRVMWAQPAVQANVATLVARGVQVLGPDAGDQACGETGAGRMWEAPAIAQAVLAPMQGTAWAGRRVLITAGPTYEDLDPVRYLGNRSSGRMGYAVAAAAAAQGAEVTLVSGPVTLATPPGVRRIDVRSAEAMHAAVLDALPGQHVFIATAAVADFRPRERQASKIKKQAGSGLALDLVQNPDILADVAARQPRPFVVGFAAETDDVERYARDKLVRKKLDMIAANRVGTDDCGFDSDRNALAVLWHDGGAQVDMADKATVARRLLELILARLPDGA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must possess FMN binding, phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity, metal ion binding to support pantothenate catabolic process, coenzyme A biosynthetic process under certain conditions.2. The protein with the reaction H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2, the Proton donor must be optimized to ensure efficient catalysis.3. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.4. A protein that is capable of catalyzing reactions within the CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis, Cofactor biosynthesis pathway with high efficiency and specificity.5. The CTP binding site should be stable and able to withstand changes in environmental conditions.6. The designed protein have DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains with specific sequence motifs that are crucial for its activity.7. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.. The designed protein sequence is ",
    "response#1": "MSGKHVLITLGPTREKWDAVRFWSNPSSGLMGACIAMAAWLRGAKVTVVSGPVNWWFPEDINVIKVNSAQQMFDAATEVWPSCTTGCFTAAVADFKPIPHGEGKFKKEGSSALRVDFDTNPDILKTIGSMKKDDQQLIGFAAETSNIQEAAKGKLERKNLDLIVANPINKPGAGFESSTNSVYLHTASG",
    "response#2": "MSGKHVLITLGPTREKWDAVRFWSNPSSGLMGACIAMAAWLRGAKVTVVSGPVNWWFPEDINVIKVNSAQQMFDAATEVWPSCTTGCFTAAVADFKPIPHGEGKFKKEGSSALRVDFDTNPDILKTIGSMKKDDQQLIGFAAETSNIQEAAKGKLERKNLDLIVANPINKPGAGFESSTNSVYLAGEKLLITAGPNHEPLDPVRYLGSRSSGKMGYALAQVGLRRGAEVTLVSGPTTLPLPQGAKVVRVGTAAEMRAAVLKEFPQATVVLMAAAVADYRPERIAPHKLKKTETTIHLTLKPNADILKELGAKKNGRMLIGFAAETEELVANAKKKLREKNLDIIVANNVAEEGSGFERDTNIATVGAAEMVEAEFKILITAGGTREQIDPVRFITNKSTGKQGYALAEAAVARGATVTLVTTVDREAPIGVEIQHAESASDLRSAVMSCADNYDVIIMAAAVADFQPSLPADRKLKKSQQITNLEIEPTHDFLIDLGKGKSSNQIIVGFAAETEELEKNAKEKLERKNLDLIVANDVTAPGTGFGYDTNEVLLIDTAGNIVELPRASKFALAHRLWDSVVARVTR",
    "response#3": "MSGKHVLITLGPTREKWDAVRFWSNPSSGLMGACIAMAAWLRGAKVTVVSGPVNWWFPEDINVIKVNSAQQMFDAATEVWPSCTTGCFTAAVADFKPIPHGEGKFKKEGSSALRVDFDTNPDILKTIGSMKKDDQQLIGFAAETSNIQEAAKGKLERKNLDLIVANPINKPGAGFESSTNSVYAEFKILITAGGTREQIDPVRFITNKSTGKQGYALAEAAVARGATVTLVTTVDREAPIGVEIQHAESASDLRSAVMSCADNYDVIIMAAAVADFQPSLPADRKLKKSQQITNLEIEPTHDFLIDLGKGKSSNQIIVGFAAETEELEKNAKEKLERKNLDLIVANDVTAPGTGFGYDTNEVLLLENNDIKLQGIRVLVTAGATREYIDPVRFITNRSSGKMGYAIAEAAAHRGAEVRLISGAATLPPPTHIKTQHVETTIQMYNEVLQAFDETDIVIMAAAPADYRPREFTPHKIKKTADPLTLPLERNPDIAEALGKQKTHQILVCFAAETDDLLENAKKKLIRKNCDLIVANDILAEGAGFQSDTNIVTLITKDNEIKIPRSTKIEVASRILDLALKLKNE"
  },
  {
    "reference": "MASRAEPTTADPGPITLIPSGAPQLDASEDFEVLDGAEEEEDDDDLSELPPLEDLGAAPNEGPTESHPASSEETEQSESPQEEWLDILGNGLLKKKILVAAPPGAERPTKGQDVTVHLKISLEEDGTQVEEQPSITFTLGDCDFLQALDLSVQLMNVGETALIIADAKYCFGSHGRSPTIPPNSTLRLEVALQTAVDGPDLELLNGRERITLADHKRECGNGHYQRADFVLAANSYDLALKAISASSKVDVSPEEEAELLELKVKCLNNLAASQLKLDHYSAALQSCNLALSHQPDNIKALFRKGKVLAQQGEYAEAIPILRAALKLEPSNKTIHSELSKLVKKHAAQRHTETAMYKKMLGTAGGSGLPAKSTTKGPRSIPWKWLFGATAVALGGVALSVVIAARN",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The desired protein should be able to perform [protein]-peptidylproline (omega=180) = [protein]-   peptidylproline (omega=0)2. The designed protein must be able to regulate positive regulation of BMP signaling pathway, neuron fate specification.3. The protein design should emphasize Acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "VSRVSSASDYNSSELKTACRKHELYVSFQDLGWQDWIIAPKGYAANYCDGECSFPLNAHMNATNHAIVQTLVHLMNPEYVPKPCCAPTKLNAISVLYFDDNSNVILKKYRNMVVRACGCS",
    "response#2": "PEIVDPASELTAGVPNKVGTCVSEGGYPAGTINWHLDGKHLVSDGKGVSVKEETRRHPETGLFTVQSELMVTPALGGAPRPTFSCSFSPGLPRRR",
    "response#3": "LTRRENKQLQRTTSDLFRLIPFSVFIIVPFMELLLPLFIKFFPGMLPSTFQTSTDRQEKLRQSLSVRLEVAKFLQQTLDQMPVQHKEHSSEEAKQFEAFFTKIRNPTEPVSNDEIIKFAKRFDDEITLDSLSREQLAALCRVLELNTIGTTTLLRFQLRLKLRSLATDDRVIAREGVDSLDLLELQQACKARGMRAYGLTEERLRFQLKEWIDLSLNEQVPPTLLLLSRTY"
  },
  {
    "reference": "MAAEKRAEGSVLGGGGGGESQEAPGPLSRLDIRRYSRQLVLPELGVSGQLRLSGSSVLVVGCGGLGCPVAQYLAAAGVGRLGLVDPDVVELDNLHRQVLHRESRLGTPKARSAASALGELNSRVRCVPYELALSPSNALQLVRDHDVVADCSDNVPTRYLVNDACVLAGKPLVSGSALRLEGQLAVYNHRGGPCYRCLFPKPPPPETVTNCADGGVLGVVPGILGTLQALEVIKIASGMGARSHGSMLLFDALEGRFRHIKLRPRDPRCAVCGDHPTVTALQDYEEFCRSSATDKCRALHLLSGEERVSVEEYKRVLDSRAPHVLVDVRPPVEVDICRLPHSLHVPLSKLEGGDEGSLKSLQERIHEARQGADDSTALPLYVVCKLGNDSQKAVRILQGLSQNGLGPISVKDIRGGLMAWASKIDEEFPQY",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that is capable of catalyzing reactions within the 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis, tRNA modification pathway with high efficiency and specificity.2. The Zn(2+), ATP binding site should be stable and able to withstand changes in environmental conditions.3. The Cysteine persulfide intermediate; for sulfurtransferase activity, Glycyl thioester intermediate; for adenylyltransferase activity of the protein must be engineered to enable effective catalysis of [molybdopterin-synthase sulfur-carrier protein]-C-terminal   Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] =   [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-NH-   CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase].4. The designed protein should be involved in Mo-molybdopterin cofactor biosynthetic process, tRNA wobble position uridine thiolation by localizing to cytosol and exhibiting molybdopterin-synthase sulfurtransferase activity, thiosulfate sulfurtransferase activity, metal ion binding, molybdopterin-synthase adenylyltransferase activity, ATP binding. The designed protein sequence is ",
    "response#1": "MPYSRHLLLPDFGVEGQRKLSQSSILVVGAGGLGSPLALYLAACGVGCLGIVDGDDVELNNLHRQIIHKEAYVGQSKVKSAADACREINSSIKVVEHHHTLKPCNALEIARKYDIVVDATDNLPTRYMISDCCVLLNKPLVSGAALGLEGQLTVYHHNGSPCYRCLFPTPPPVAACQRCSDSGVLGVVPGVIGCLQALEAIKVATGVGEPLCGRMLLFDALSARIRVVKLRGSSPDCAVCGEAPTVTSRLNPEIQTNIYQTHLTPQNAEDIVQKYDLILDCTDHPTSRYLISDICVLLQKPLISASALRTDGQLIVLNHPPLPVGNISGGPCYRCVFPKPPPPESVVSCGDGGIIGPVVGTMGVLQALEAIKFIASGKLEVVHQDEGKTNPTSMLLFSTNSTPPFRNVRLRARRPACHSYDIIVDATDNAPSRYMINDCCVVLGKPLVSGAAVGLEGQLTVFNYNGGPCYRCLFPTPPPTTACQRCSDAGVLGVVPGVIGCLQALEAIKIASAVGEPLSERMLLFDALSGRIRNVKIRGRSPQCSRLNPEIQTNIYQTHLTPQNAEDIVQKYDLILDCTDHPTSRYLISDICVLLQKPLISASALRTDGQLIVLNHPPLPVGNISGGPCYRCVFPKPPPPESVVSCGDGGIIGPVVGTMGVLQALEAIKFIASGKLEVVHQDEGKTNPTSMLLFSTNSTPPFRNVRLRARRPASRLNPEIQTNIYQTHLTPQNAEDIVQKYDLILDCTDHPTSRYLISDICVLLQKPLISASALRTDGQLIVLNHPPLPVGNISGGPCYRCVFPKPPPPESVVSCGDGGIIGPVVGTMGVLQALEAIKFIASGKLEVVHQDEGKTNPTSMLLFSTNSTPPFRNVRLRARRPA",
    "response#2": "SGAPHLLLDVRPQVEVDICRLPHAVHIPLKHLERGDAESLKLLGEAIREEKRGTEEGPELSIYVICKLGNDSQKAVKILQSLAAAHELGSFTVRDVVGGLMAWAAKIDGTFE",
    "response#3": "MSYGRQMIVPDIGIQGQLRLKSASVLLVGGGGLGCPAAAYIAGAGVGTIGIVDGDTVEESNLHRQILHNTYKVGVNKAASIARYISSYESQEVAGYSRQIILHSFGVQAQARLCRGSVLIIGAGGLGSPAALYLAAAGVGRLGIVDRDVVELSNIHRQVIHKEASVGVHKALSAAEACRALNSSILVETYLEGLKPDNAVELISEYDVIVDASDNAPTRYLISDACCVARRPLVSGAAIGTDGQLTVYCHGEDGPCYRCLFPESPAAGNCARCVDAGVLGVVPGVIGTLQALEAVKLLSGVGDTLSRRLLAIDCAAGRFHSVKLRAKSPACHSYDIIVDATDNAPSRYMINDCCVVLGKPLVSGAAVGLEGQLTVFNYNGGPCYRCLFPTPPPTTACQRCSDAGVLGVVPGVIGCLQALEAIKIASAVGEPLSERMLLFDALSGRIRNVKIRGRSPQCYSRHLLLPSFGVQAQSNLLKSSILVVGAGGLGSPALLYLAACGVGRLGIVDHDVVELNNMHRQVIHTEAYIGQPKVKSAAAACRSINSTIQIVEHKQALRTSNALEILSQYDIVVDATDNAPSRYMISDCCVVLGKPLVSGAALGLEGQLTVYNYKGGPCYRCLFPTPPPTTACQRCSDSGVLGVVPGIIGCLQALEAIKIASDIGEPLSGRMLLFDALSARIRIVKIRGRSLQCEYSRHLLLPSFGVQAQSNLLKSSILVVGAGGLGSPALLYLAACGVGRLGIVDHDVVELNNMHRQVIHTEAYIGQPKVKSAAAACRSINSTIQIVEHKQALRTSNALEILSQYDIVVDATDNAPSRYMISDCCVVLGKPLVSGAALGLEGQLTVYNYKGGPCYRCLFPTPPPTTACQRCSDSGVLGVVPGIIGCLQALEAIKIASDIGEPLSGRMLLFDALSARIRIVKIRGRSLQCE"
  },
  {
    "reference": "MGSKWSKSSIVGWPDVRERIRRTEPAAEGVGAASQDLEKHGALTTSNTGNNNADCAWLQAQEEEEEVGFPVRPQVPLRPLTYKGAFDLGFFLKEKGGLDGLIWSKKRQEILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPKEVEEANEGENNCLLHPMSQHGMEDEEREVLKWMFDSSLARRHLAREKHPEFYKDC",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be expressed specifically in the extracellular region The designed protein sequence is ",
    "response#1": "GKWYGIGLASNSNWFQSKKQQLKMCTTVITPTADGNLDVVATFPKLDRCEKKSMTYIKTEQPGRFLSKSPRYGSDHVIRVVESNYDEYTLMHTIKTKGNEVNTIVSLFGRRKTLSPELLDKFQQFAKEQGLTDDNILILPQTI",
    "response#2": "LMQFETLIMKIAGRSGVWWYGSYGCYCGAGGQGRPQDPSDRCCFVHDCCYGKVTGCNTKDEFYTYSEENGAITCGGENPCLKEVCECDLAAAICFRDNLDTYNSC",
    "response#3": "HLMQFETLIMKIAGRSGVWWYGSYGCYCGAGGQGRPQDPSDRCCFVHDCCYGKVTGCNTKDEFYTYSEENGAITCGGENPCLKEVCECDLAAAICFRDNLDTYNSKKYWMFPAKNCG"
  },
  {
    "reference": "MKYIFVTGGVVSGLGKGICAASLGRLLKQCGLRVRNQKFDPYFNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFVDESLDGNASVSSGKVFWSVLNRERQGGYLGGTVQIIPHVTDEIKRRIYAMEDGRTDVVISEIGGTVGDIESQPFLEAIRQVATEKGRENVLYIHVPLIVSIPGSGELKSKPTQHSVKELLSVGIQPDILVCRTDAPITQEIRHKIALFCNVQEDCVIPNVTASTLYEVPLLLEKEGLSNVVCRKLGLGSLRPDMTEWAAMVQKIKQVHRHVEIALVGKYTGLRDAYLSVAEALFHAGTACDAEVSIRWIDSETLHPENIEQALAGCGGVLVPGGFGDRGIEGMILAARYARENGVPYFGICLGMQIAVIEFARHAAGWDDANSAEFSGTTAHPVIALMPDQVGITQKGGTMRLGRYPCVLAEDSASRRLYGAEEISERHRHRYEFNNEFRTELQAYGLRLAGTSPDGSLVEIVELPDHPWFVGCQFHPEFKSRPNRPHPLFRGFVAAALKREEQV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.2. The protein must exhibit metal ion binding, CTP synthase activity, ATP binding.3. The designed protein should be able to compete with endogenous proteins or inhibitors for UTP, ATP, CTP binding. The designed protein sequence is ",
    "response#1": "LSDAYISVVEALKHAAIAMESELKLRWVSSEDIESYGAEDFLSNVSGILVPGGFGVRGVDGKIAAIEYARTKEIPFLGLCLGMQCSVIEWARNVAGLENAHSVEFAADVAHPVINLLPEQQDVVDLGGTMRLGLYPCRILPNTLAASLYQKEVIYERHRHRYEFNNAYRNLFLETGYMISGTSPDNRLVEMIELPTHPFFLATQFHPEFASRPSSPHPLFKGFVEY",
    "response#2": "VKIAVVGKYVALPDAYMSVTEALHHGGIEHDAQVKITWINAEELEEPNADLDEIFVGCKGILVPGGFGDRGVEGKIRAIQYAREHEIPFLGLCLGMQCAVIEFARHVAGLTNAHSTEFVPETPHPVIALMEDQQDVEEKGGTMRLGAYPCLLADGSHSRAEYGAGEISERHRHRFEFNNAYRAQLEEKGMVIAGTSPDDRLVEVVEIADHPWFVASQFHPELKSRPNHPHPLFAGFIQK",
    "response#3": "IEIALVGKYVALHDAYISVTEALRHAGLYHGVDVHIRWVNAEEVEKRGPEALLQGVRGIVIPGGFGHRGVEGKVEAAGYARRKNLPFLGLCLGLQCATIEFARNVLGLADANSTEFDPHTSAPVIDLMADQRDVYEKGGTMRLGAYPCKLVPGTRAAAAYGVELVHERHRHRFEFNNRYREALEAAGLIVSGTSPDGRLVEIAELRDHPWFVGTQFHPEFRSRPTRPHPLFRDFLRAA"
  },
  {
    "reference": "MSQFNNVSIVKKANVYFDGNVTSRAVIFPDGSKKTLGIMQPGEYEFGTEEKELMEILAGDLEILLPGSTDWQKVSGGESFEVPARSKFGIRIKSITDYCCSYLTD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must be able to thymidine phosphorylase activity, uridine phosphorylase activity, guanosine phosphorylase activity.2. For general function, the protein need meet that Catalyzes the phosphorolysis of diverse nucleosides, yielding D-ribose 1-phosphate and the respective free bases The designed protein sequence is ",
    "response#1": "MGQ",
    "response#2": "ML",
    "response#3": "MILVG"
  },
  {
    "reference": "MDSQIKHAVVVKVMGRTGSRGQVTQVRVKFTDSDRYIMRNVKGPVREGDILTLLESEREARRLR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be able to modulate ribosomal small subunit assembly, maturation of SSU-rRNA in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "GIDRPLRNEKDFERFAGELVDITLKAPIGVAASAGSAVSANRKKFRGTLERAAPKDGVAGWQIVWSDEPPVKPGQKVSKKKLPAPLQALGFTLDEIHQARLAG",
    "response#2": "GIDRPLRNEKDFERFAGELVDITLKAPIGVAASAGSAVSANRKKFRGTLERAAPKDGVAGWQIVWSDEPPVKPGQKVSKKKLPAPLQALGFTLDEIHQARLAG",
    "response#3": "GIDRPLRNEKDFERFAGELVDITLKAPIGVAASAGSAVSANRKKFRGTLERAAPKDGVAGWQIVWSDEPPVKPGQKVSKKKLPAPLQALGFTLDEIHQARLAG"
  },
  {
    "reference": "MEIPKLLPARGTLQGGGGGGIPAGGGRVHRGPDSPAGQVPTRRLLLPRGPQDGGPGRRREEASTASRGPGPSLFAPRPHQPSGGGDDFFLVLLDPVGGDVETAGSGQAAGPVLREEAKAGPGLQGDESGANPAGCSAQGPHCLSAVPTPAPISAPGPAAAFAGTVTIHNQDLLLRFENGVLTLATPPPHAWEPGAAPAQQPRCLIAPQAGFPQAAHPGDCPELRSDLLLAEPAEPAPAPAPQEEAEGLAAALGPRGLLGSGPGVVLYLCPEALCGQTFAKKHQLKMHLLTHSSSQGQRPFKCPLGGCGWTFTTSYKLKRHLQSHDKLRPFGCPAEGCGKSFTTVYNLKAHMKGHEQENSFKCEVCEESFPTQAKLGAHQRSHFEPERPYQCAFSGCKKTFITVSALFSHNRAHFREQELFSCSFPGCSKQYDKACRLKIHLRSHTGERPFLCDFDGCGWNFTSMSKLLRHKRKHDDDRRFMCPVEGCGKSFTRAEHLKGHSITHLGTKPFVCPVAGCCARFSARSSLYIHSKKHLQDVDTWKSRCPISSCNKLFTSKHSMKTHMVKRHKVGQDLLAQLEAANSLTPSSELTSQRQNDLSDAEIVSLFSDVPDSTSAALLDTALVNSGILTIDVASVSSTLAGHLPANNNNSVGQAVDPPSLMATSDPPQSLDTSLFFGTAATGFQQSSLNMDEVSSVSVGPLGSLDSLAMKNSSPEPQALTPSSKLTVDTDTLTPSSTLCENSVSELLTPAKAEWSVHPNSDFFGQEG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Cooperates with CIITA to promote transcription of MHC class I and MHC class II genes.2. The designed protein must possess transcription coregulator activity, C2H2 zinc finger domain binding, transcription coactivator activity, metal ion binding to support regulation of transcription by RNA polymerase II, positive regulation of DNA-templated transcription under certain conditions. The designed protein sequence is ",
    "response#1": "RMRPWLEEQINSNTIPGLKWINKEKKIFQIPWMHAARHGWDVEKDAPLFRNWAIHTGKYQSGVDKPDPKTWKANFRCAMNSLPDIEEVKDKSIKKGNNAFRVYRML",
    "response#2": "RMRPWLEEQINSNTIPGLKWINKEKKIFQIPWMHAARHGWDVEKDAPLFRNWAIHTGKYQSGVDKPDPKTWKANFRCAMNSLPDIEEVKDKSIKKGNNAFRVYRMK",
    "response#3": "RMRMRPWLEEQINSNTIPGLKWINKEKKIFQIPWMHAARHGWDVEKDAPLFRNWAIHTGKYQSGVDKPDPKTWKANFRCAMNSLPDIEEVKDKSIKKGNNAFRVYRMLG"
  },
  {
    "reference": "MESEKNMEHKIVFATSNAGKMREIREILKDLGAEILSMKEAGVDVDIVEDGDTFAANALIKAKAVWEKTGGIVLADDSGLVIDALNGEPGVYSARYMGEKTSYEIKNWNLIHRLDGVPEEKRTARFVCVIAAVLPDGRTLTAEGTMEGVIAHEPAGAGGFGYDPILMLPEYGKTSAEITMDEKNAISHRGKALRAMKKCLAEEIGE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.2. A protein with dITP diphosphatase activity, XTP diphosphatase activity is needed to enhance purine nucleoside triphosphate catabolic process, nucleotide metabolic process in a specific context.3. The protein should be designed to catalyze the following reaction dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "DHHHHHH",
    "response#2": "TPCTAPDE",
    "response#3": "LPVLFDS"
  },
  {
    "reference": "MVPTGQVAEKQAYEEPRQDHELKSWRCLVFYLCFFGFMAQLRPGESFITPFLLERKFTKEQVTNEIIPMLPYSHLAVLVPVFLLTDYLRYKPVLVLQCLSFVCVWLLLLLGTSVVHMQLMEVFYSVTMAARIAYSSYIFSLVHPSRYQRMASYSRAAVLLGVFISSVLGQALVTVGHISTYTLNCVSLGFILFSLVLSLFLKRPKRSLFFNRSTLARGALPCELDQMHPGPDRPETRKLDRMLGTCRDSFLVRMLSELVENARQPQLRLWCLWWVFNSSGYYLITYYVHVLWRSTDSSLSYNGAVDAASTLLSAITSFSAGFLSIRWTLWSKLVIAGVIAIQASLVFCMFQIRDIWVCYVTFVLFRGAYQFLVPIATFQIASSLSKELCALVFGINTFLATALKTCITLVVSDKRGLGLQVRDQFRIYFIYFLMLSITCFAWAGLDGLRYCQRGRHQPLAQAQELRSPLETSVQAISLQDGDLRGPQPSAPQLLSEDGMEDDRGDLRVEAKA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Extracellular, Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.2. The protein should have a Helical; Name=10, Helical; Name=3, Helical; Name=5, Helical; Name=2, Helical; Name=9 domain that is long enough to span the entire membrane.3. The designed protein should be involved in folate import across plasma membrane by localizing to basolateral plasma membrane, brush border membrane, apical plasma membrane, plasma membrane and exhibiting organic anion transmembrane transporter activity, xenobiotic transmembrane transporter activity, antiporter activity.4. The protein must exhibit the following characteristics: Antiporter that mediates the import of reduced folates; Mechanistically, acts as a secondary active transporter, which exports intracellular organic anions down their concentration gradients to facilitate the uptake of its substrates ; Has high affinity for N5-methyltetrahydrofolate, the predominant circulating form of folate.5. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "QWQLLHLNGTFPNTNEPDTEPCVDGWVYDRSSFLSTIVTEWDLVCESQSLKSMVQSLFMAGSLLGGLIYGHLSDRVGRKIICKLCFLQLAISNTCAAFAPTFLVYCILRFLAGFSTMTILGNTFILSLEWTLPRSRSMTIMVLLCSYSVGQMLLGGLAFAIQDWHILQLTVSTPIIVLFLSSWKMVESARWLIINNQLDEGLKELRRVAHINGKKNTEETLTTELVRSTMKKELDAVRIKTSIFSLFRAPKLRMRVFGLCFVRFAITVPFYGLILNLQHLGSNVSLFQILCGAVTFTARCVSLLTLNHMGRRISQILFTFPVGLFILVNTFLPQEMQILRVVLATLGIGSVSAASNSASVHHNELVPTILRSTVAGINAVSGRTGAALAPLLMTLMAYSPHLPWISYGVFPILAVPVILLLPETRRRELTDTIRRGTLTTGPTSDLGFNNMVNQGRIGVSKAMNIEDSRIFIVSGRNETYALLLPLIQNDDIDLVICSSQDHGDACKEIAAMYIDSPTIKTQFLVRGSGAATKNLIQITYNYASVNYISGMFAGLQTVKNKIGFLSPGSAANNNDSFVYAFWIGAKQVNPDVKFYYYNIGSYLDQDKTIAATQDLINIYGCDVIADTLDDFSAGNVAIQNNQYAMGTNGFPQRDVYGENVIFSYAYNWTKYFLPIAGSVANGR",
    "response#2": "QWQLLHLNGTFPNTNEPDTEPCVDGWVYDRSSFLSTIVTEWDLVCESQSLKSMVQSLFMAGSLLGGLIYGHLSDRVGRKIICKLCFLQLAISNTCAAFAPTFLVYCILRFLAGFSTMTILGNTFILSLEWTLPRSRSMTIMVLLCSYSVGQMLLGGLAFAIQDWHILQLTVSTPIIVLFLSSWKMVESARWLIINNQLDEGLKELRRVAHINGKKNTEETLTTELVRSTMKKELDAVRIKTSIFSLFRAPKLRMRVFGLCFVRFAITVPFYGLILNLQHLGSNVSLFQILCGAVTFTARCVSLLTLNHMGRRISQILFTFPVGLFILVNTFLPQEMQILRVVLATLGIGSVSAASNSASVHHNELVPTILRSTVAGINAVSGRTGAALAPLLMTLMAYSPHLPWISYGVFPILAVPVILLLPETKSIPLEAMDSLFRRRWFKVPALPLLPLMSIFVNIYLMMQMTAGTWARFGVWMLIGFAIYFGYGIQHG",
    "response#3": "LSFHNISYQETVQSGFPLRKKAYVIERLSNISGIMKPGLNAIMGPQDGSRSLLLDVLAARRDPRGLSGDILINGKPRPANFKCTSGYVPQNDVVLGTVTVRDNLEFSAALRLPVTITRDEKRRRINEVLELLHLNKEQNIKPRSKELRKRTSIAMELVTEHPILFLDDPTTGLDLRTTTDVILVLRRMSKKGRTIIFSINQPQYSIFKFFDSLTLVASGKVMFHGPAQDALEYFRSAGYNYELSFHNISYQETVQSGFPLRKKAYVIERLSNISGIMKPGLNAIMGPQDGSRSLLLDVLAARRDPRGLSGDILINGKPRPANFKCTSGYVPQNDVVLGTVTVRDNLEFSAALRLPVTITRDEKRRRINEVLELLHLNKEQNIKPRSKELRKRTSIAMELVTEHPILFLDDPTTGLDLRTTTDVILVLRRMSKKGRTIIFSINQPQYSIFKFFDSLTLVASGKVMFHGPAQDALEYFRSAGYNYEEGTIFGVIGSSGAGKSTLIRCVNMLEAPTSGSIVVDGVDLTKLSKKQLSETRRNIGMIFQHFNLLSSRTVFDNVALPLELAGKDKEQIQSKVTELLKLVGLADKHESYPANLSGGQKQRVAIARALASDPKVLLCDEATSALDPATTQSILELLKEINRKLKITILLITHEMDVVKSICHEVAIIGGGELVEKGTVGEIFAHPKTSYPLVRLEFTGATVDAPLVSQISRKYNIDISILSSDLDYAGGVKFGMMVAELFGNEEDDNAAIEYLREHNVKVEV"
  },
  {
    "reference": "MIKYFENKKIVIGVTGSIAAYKSVDIASQLTQRGAIVDVIMTEKATKFVSPLSFQAITHRKVVVDLYDPASDMGMDHLVLANQADLFVIAPATANIVSKTALGISDNSLTATLLATEAPILICPAMDGDMFQKPQIKTNLDKLIEYGFHMLGPDEGRLASGKVGKGRLVESSEILDMCRYILGIHGDYEGKNIVVTSGGTREHIDPVRYISNNSSGKMGNFLAEAARDRGAFVQLVSSSTVSKQSPGIELKKVKTAEEMLQSVELASTSADVLIMAAAVSDWKPSDVSQQKIKKDGVKEISIEMVQSSDILSTVLKPGLIKIGFAAETQDVIKNALEKLNSKGLDLIVANDISDESIGFESDYNSVAIIDKNGNTKQVDRSLKYEVANLILDEVLNLFD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The desired protein should be able to perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO22. We require a protein that can specifically bind to the metabolites produced in the CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis pathway. The designed protein sequence is ",
    "response#1": "LQGKKVIVTAGGTREAIDPVRFIGNRSSGKMGFALAEAAWLRGADVVLISGPVGLAPPFGVTCHYVESAAQMRQLVLDHYEEADIVVKAAAVADYRVANPGTQKIKKTDNDLELLLTKNPDILKELGEKKRHQVLIGFAAETEELLANAQEKLVKKNLDLIVANDVTRQDAGFNADTNEVTLFS",
    "response#2": "STKRVLVTAGGTREPIDPVRFIGNRSSGKMGIAMADAAFARGADVTLISTVDVERAYPVVVVETAQEMQDAVESEFDGCDALVMTAAVADFRPLAVSSQKIKKTESEDLVLELTKNPDIIDLLGRVKRKDQVIVGFAAESEALLSNAAQKLKRKNLDMIIGNDISVPDIGFGSDDNAVVILYDDAGHNRQIPRAPKTDLARALIAHIAARLG",
    "response#3": "LQGKKVIVTAGGTREAIDPVRFIGNRSSGKMGFALAEAAWLRGADVVLISGPVGLAPPFGVTCHYVESAAQMRQLVLDHYEEADIVVKAAAVADYRVANPGTQKIKKTDNDLELLLTKNPDILKELGEKKRHQVLIGFAAETEELLANAQEKLVKKNLDLIVANDVTRQDAGFNADTNEVTLIG"
  },
  {
    "reference": "MGPYLPAILRPIGRYATLYNVPRVAGQLRCYAADAKDAADAQGESAADPRVAELEKQLADKSKEAADLKDRLLRSVADFRNLQEVTRRDVQKARDFALQRFSKDLLESLDNFGHALGAVSPEALQRSPEIADLHAGVRLTRDVFEKTLLKHGIAPIDALGQPFDPNLHEATFELPQPDKTPGTVFHVQQPGYTLNGRVIRPAKVGVVKDPDA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner; Seems to control the nucleotide-dependent binding of SSC1 to substrate proteins ..2. The ideal protein would be one that plays a critical role in protein import into mitochondrial matrix through chaperone binding, protein homodimerization activity within PAM complex, Tim23 associated import motor. The designed protein sequence is ",
    "response#1": "VSEVLTEIHKIDANFDKQEWLRFCETKIIPNILEAFIRFDLEVLQSWCHERAYTQLSTVVKEYQKMHFSTKDSRIIDINKVEMATGKMMEQGPVLIISFQVYMINVTKNADGKVVEGDPDNPKRINHIWVLCRDVEEYNPALAWKLLEVHS",
    "response#2": "VSEVLTEIHKIDANFDKQEWLRFCETKIIPNILEAFIRFDLEVLQSWCHERAYTQLSTVVKEYQKMHFSTKDSRIIDINKVEMATGKMMEQGPVLIISFQVYMINVTKNADGKVVEGDPDNPKRINHIWVLCRDVEEYNPALAWKLLEVHS",
    "response#3": "SEVLTEIHKIDANFDKQEWLRFCETKIIPNILEAFIRFDLEVLQSWCHERAYTQLSTVVKEYQKMHFSTKDSRIIDINKVEMATGKMMEQGPVLIISFQVYMINVTKNADGKVVEGDPDNPKRINHIWVLCRDVEEYNPALAWKLLEVT"
  },
  {
    "reference": "MKPNIHPAYRTVVFHDTSANEYFKVGSTIKTEREIELEGVTYPYVTIEVSSKSHPYYTGKQKTFDNEGSAARFQKRFGNFIGAKRG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be optimized for ribosome, ribonucleoprotein complex-specific folding and stability, while maintaining structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "MEKIRVHVPLHFINQESSVGVKKGGVVSHGMVDVEVDCLPKDLPEYIEVDLAQVDIGGIIHLSDLKLPAGVEIHALAQGVGHDLPVASVHAPL",
    "response#2": "MGRGNRICFGRFALQALEPAWITSGQIEAGRRTINRYARRGGKIWVRIFPDKPITMRPAETRMGSGKGSPEYWVSVIRPGRILYEMGGVSETVARAAARIAAYKMPIRTQFS",
    "response#3": "MGLVFEAELRHQLGKGASRRLRHAGKVPAVLYGGGGEPVSLLLDHHKVVKNLENEATYSHVLTIRFDGREENAILKAVQRHPAKPVVMHLDFQR"
  },
  {
    "reference": "MALKVVILAAGKGTRMRSSLPKVLQPLAGQPLLQHVVNTAKLLDAKQVLTVVGHQSHLVEERMAGQNIDFVAQTEQLGTGHAVQQVVPEIADNDTVLVLYGDVPLTAKTTLQDLLELVTEQHPLALLTINLDNPTGYGRIVRDNHLAVQAIVEQKDASNEQLQIQEVNTGILAARGKQLKLWLSQLQSNNAQGEFYLTDIIAMCVADGFAVQTTQPACEMEVLGVNDKRQLQALERQYQLSIADDLMVQGATLIDASRLDVRGTVALGQDIEIDVNVILAGKVVLADGVKIGANCILINVTIGANTEIHPFSHLEDCVVGASTNIGPYARLRPGTELADKVRIGNFVETKKAKIGTGSKVNHLSYVGDTIMGEGCNIGAGTITCNYDGVNKHQTVIGDNVFVGSDTQLVAPVEIGDNATIGAGSTITKGCPADELSLSRSKQLTIKGWQKPEKKQ",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have a high affinity for acetyl-CoA, Mg(2+).2. A protein that can modulate the overall flux of metabolites through the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway, enhancing or suppressing its activity as needed.3. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).4. The designed protein should be optimized for glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity, magnesium ion binding activity in order to improve cell morphogenesis, lipid A biosynthetic process, cell wall organization, UDP-N-acetylglucosamine biosynthetic process.5. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "LVLAAGAGTRMKSAKQKTLHEIGGRSLLGHSLHAAAGLNPAHLVAVVGHQREQVAPAVEAIAAELDREVLVAVQEEQNGTGDAVAAGLAPLPDFNGTVIVTNGDVPLLRPETIQSLFEAHVAESNAVTVLTAVLDDPTGYGRIVRDEAGAVLAIVEHKDASEAQRAVREINSGIYAFDADVLRDALGKISTDNSQGEEYLTDVLGIARADGRRVGAHVVDDAWLTEGVNDRVQLATLGRELNRRLLERWMRAGVTLADATRIDVRGEISAERDTFIDVNTVFEGNVRIGEGSKIGPNCVLKNVVLGKNCEVYANSVLEGCEIAENCQIGPFARLRPGTKLAKHCKIGNFVETKNAVFDESSKANHLTYLGDVTLGKNVNVGAGTITCNYDGAYKHQTVIEDGVFIGSDTQLVAPVTVGENATIGAGSTIRKNVPAGELTLTVSTGRRSIEGWVERRRRPGSAAAEAAARALAAADPTDPHTDAAGTTPHTDATNPHTDTPNTETTNPHTDTPNTHTDTANPGTDGAQAGGR",
    "response#2": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVTTTMPDPTGYGRIVRNGNNLVTSIVEQKDASEYQKSIDEINTGIYCVDNKWLWESLSHITNDNAQQEYYLTDIVKLAVSDGHAINPVKPNDFAETLGVNDRVQLAAVEREYQLINARLLMKAGVMLMDPARIDVRGELICGADVEIDVNCIFEGTVKLGNRVKVKANCILKDVTVSEGSTIHPFSHVEEAKIGQNCKIGPYARIRPETQLIDNVHIGNFVEVKKSQIASGSKVNHLSYIGDTIIGQNVNIGAGTITCNYDGAYKHQTIIEDNVFVGSDTQLVAPVKVSYGSTIGAGSTITRDTPENELTLSRSKQIHIENWKRPVKKK",
    "response#3": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLTAEVDDPTGYGRILRDEHGAVQRIVEQKDASAEERAVREINTGLMAVPNRHLGDWLARLRNDNAQGEYYLTDVIALAVADGLSVVSTQPDASWETLGVNSRVQQAELERAWQLRQAQALMEQLAEAEQVMQARLRKAALDSGVTMTAPETVFLAADTSFGKDVTIEPFVVIGPGVTIADGAVIHAFSHIMQASIGSNASVGPYARLRPGTSLGAGAKIGNFVETKAATLEAGAKVNHLTYVGDAHVGANANIGAGTITCNYDGFNKYRTEIGEGAFIGSNSSLVAPVKVGAGAYVGSGSVVTRNVPDDALAVGRGQQRNIAGWQRPVKR"
  },
  {
    "reference": "MPAKEKTFEESLNALEEIVQRLERGDVPLEEALAAFQEGMALSKQCQDTLEKAEKTLTKMMTENNEEIVFEESEEA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The ideal protein would be one that plays a critical role in DNA catabolic process through exodeoxyribonuclease VII activity within cytoplasm, exodeoxyribonuclease VII complex.2. We need a protein that can perform Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-   direction to yield nucleoside 5'-phosphates. with high efficiency. The designed protein sequence is ",
    "response#1": "LFALDRKKPLPFLPQLVGLVCGRASAAERDVLENARRRWPAVRFEVRNTAVQGVNAVAQVVQAVEELDALQEVDVIVVARGGGSVEDLLPFSDEQLIRTVAACRTPVVSAIGHEPDSPLLDLVADLRASTPTDAAKKVVPDVGEELERVQQLRDRALRTVRGLLDREERGLAHALGRPAMERPHRLVDEREAEVDALLGRARRVLGHLLDRADSELSHTF",
    "response#2": "LFALDRKKPLPFLPQLVGLVCGRASAAERDVLENARRRWPAVRFEVRNTAVQGVNAVAQVVQAVEELDALQEVDVIVVARGGGSVEDLLPFSDEQLIRTVAACRTPVVSAIGHEPDSPLLDLVADLRASTPTDAAKKVVPDVGEELERVQQLRDRALRTVRGLLDREERGLAHALGRPAMERPHRLVDEREAEVDALLGRARRVLGHLLDRADSELSHTV",
    "response#3": "LFALDRKKPLPFLPQLVGLVCGRASAAERDVLENARRRWPAVRFEVRNTAVQGVNAVAQVVQAVEELDALQEVDVIVVARGGGSVEDLLPFSDEQLIRTVAACRTPVVSAIGHEPDSPLLDLVADLRASTPTDAAKKVVPDVGEELERVQQLRDRALRTVRGLLDREERGLAHALGRPAMERPHRLVDEREAEVDALLGRARRVLGHLLDRADSELSHTT"
  },
  {
    "reference": "MDPVDPNLEPWNHPGSQPATPCNKCYCKKCCYHCPACFLNKGLGISYGRKKRRQRRRTPPSSEDHQNLISKQPLPQTQGDSTGPKESKKKVESKTGPDRLDW",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should include a Nuclear localization signal, RNA-binding (TAR), and protein transduction motif to ensure proper function.2. The designed protein's functionality will benefit from Basic and acidic residues, Polar residues compositional bias in its structure.3. The designed protein should have protein domain specific binding, actinin binding, trans-activation response element binding within host cell cytoplasm to regulate positive regulation of viral transcription, modulation by virus of host chromatin organization, suppression by virus of host type I interferon-mediated signaling pathway.4. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding.5. For general function, the protein need meet that This also contributes to the increase in proviral transcription rate, especially when the provirus integrates in transcriptionally silent region of the host genome; Tat can reactivate a latently infected cell by penetrating in it and transactivating its LTR promoter; In the absence of Tat, the RNA Pol II generates short or non-processive transcripts that terminate at approximately 60 bp from the initiation site; Through its interaction with host TBP, Tat may also modulate transcription initiation; To ensure maximal activation of the LTR, Tat mediates nuclear translocation of NF-kappa-B by interacting with host RELA; Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication The designed protein sequence is ",
    "response#1": "MEPVDPRLEPWKHPGSQPKTACNNCYCKKCCFHCQVCFMKKGLGISYGRKKRRQRRRTHQDSQIHQVSLSKQPSSQPRGDPTGPEEPKKKVERETETDPVH",
    "response#2": "MRVMGIQRNCQHLLRWGTMILGMIIICSTTENFXNXTXNXTXXXP",
    "response#3": "MRVMGIQKNWRHLWRWGTILFWMLMICRAAEDNXKXNXTDKXSSXDXEXGXKXRXGAXIXGFLGFLGAAGSTXXGXTHXLPXPRGPDRPEGIEEEGGERDRDRSXRLVHGFLALVWDD"
  },
  {
    "reference": "MSHSSDLIAEDIEGYLKSHEHKGLLRFITCGSVDDGKSTLIGRLLFESKLLFEDQLAAIEADSKKYGTQGGEMDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADTPGHEQYTRNMVTGASTADAAILMVDARHGILTQTRRHSFLMSLMGMRHIIVAINKMDLVDYSKARYDQIVEEYRAFAKQLGLENITFIPMSALKGDNVIEHSTKMPWYHGTTLMGYLETVEVDDERLQRSPFRMPVQWVNRPNLDFRGFTGMIASGVVRPGDPIRVQPSGKTSTVSRIYTFDGDLEEAVAGQSITLLLDDEIDISRGDIISGVDQPAHTADQFETTLVWMHEAPLLPGRPYLMKLGTQLVSATVTDIKYQVNVNTLEHTAGKQLDLNGIGVCTISLNRAVAFDAYKENQDTGGFILIDRMTNNTVGAGMLHFALRRSQNIHMQHVDIDKQARALAKGQKPAVLWFTGLSGAGKSTIANLVEKKLFGNGQHTYLLDGDNVRHGLNRDLGFTDADRVENVRRVAETAKLMVDAGLIVMSAFISPFRSERQLARDLLEEGEFIEIYVDAPLDVVEARDPKGLYKKARRGELKNFTGIDSAYEAPESPDVHLKTSEMSAEEAADAVIEALRERGLIS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be able to modulate sulfate assimilation in a way that leads to a desirable outcome.2. The protein should be optimized for activity in sulfite from sulfate: step 2/3, Sulfur metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "TPHGGKLRDLVIRDAPLKQQLLQEAKTLPALTLTARQLCDLELILNGGFSPLTGFLNQEDYNSVVNDLRLSSVKNESNGKGLLWPIPITLDVDETTSKKHSVGDRIVLIDLRDETPLAILTIESIYKPDKKLEAEKVFRGDSEHPANKYLLETAGDYYIGGELQGIL",
    "response#2": "VKPHGGTLIDRVLKGPAREEALKRAKELPRLFLDRWEASDLELIANGAFSPLAGFMNKADYENVVDNMRLADGTVWTIPIVLGVASGEAGSLAPGREAALCAEDGELLGLIKVEEIYDYDRRREAEKVYKTTDEAHPGVKRVYERAQYLLGGEISLISK",
    "response#3": "VKPHGGTLIDRVLKGPAREEALKRAKELPRLFLDRWEASDLELIANGAFSPLAGFMNKADYENVVDNMRLADGTVWTIPIVLGVASGEAGSLAPGREAALCAEDGELLGLIKVEEIYDYDRRREAEKVYKTTDEAHPGVKRVYERAQYLLGGEISLISD"
  },
  {
    "reference": "MSISSKTEDSDIRSSVMTDHGPSGMKRLAGLSLAALGVVFGDIGTSPLYAVRECFHGEYGITASAGNVLGVLSLLFWALVLIVGLKYLTFIMRADNDGEGGILALTALIITHSRNRGYERWLLVAIGLFGASLLYGDGMITPAISVLSAIEGLQIIAPAFHEMVIPLTMLVLAGLFLFQHNGTARVGALFGPIILLWFIAIAILGIIEIVKYPQVLQAMLPWHGISFLLGNNLKGFTVLGAVFLSVTGAEALYADMGHFGRRPIRITWFLLVLPALLLNYFGQGALLLSSPGEAHHPFYGLVPSWAMIPMVLLATSATIIASQALITGVFSLTQQAIQLGYLPRLTVTHTSARHMGQIYVPAANWSLMVGTIGIVAWFGSSSKLAAAYGVAVTATMLISTILFYYIARDLWKWNPAALNVMITFFAAIDLSFFGASMSKLFHGAWVPLAVALVMFTIMNTWKQGRKLLMRQLQDRTLTVDEFIRSLALQQPQRVPGQVVYLTANPDVVPIALLHNLRHNKVLHSEVALFHFSNERVPRVPNSRKIEVDRLGDGFTRVIARYGFLEYPNISQVLALANQQGLNFKPEGISFFLSREKIVAGEKTKMFPSRKKLFALMARNALSATAYYDLPSGQVIEIGVQVQI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The protein must have high specificity towards potassium ion transmembrane transporter activity.3. The protein must exhibit the following characteristics: Transport of potassium into the cell.4. We need a protein that can perform H(+)(in) + K(+)(in) = H(+)(out) + K(+)(out) with high efficiency. The designed protein sequence is ",
    "response#1": "IVYLTSDPEMKRVDTDIFFSIFADHPKRARAWWAVSVETSDNPFTREYSVENFGTDFLFRVRIRLGFKVSQSIPAYIHQIMNDLSKSGDLPKQTTRYPKLDADPNIGPIRYVLIHKALMPESKVSQKGAISLQIKYAIRHLAGSPVKWFGLAPYNPLIF",
    "response#2": "IVYLTSDPEMKRVDTDIFFSIFADHPKRARAWWAVSVETSDNPFTREYSVENFGTDFLFRVRIRLGFKVSQSIPAYIHQIMNDLSKSGDLPKQTTRYPKLDADPNIGPIRYVLIHKALMPESKVSQKGAISLQIKYAIRHLAGSPVKWFGLAPYNPLIF",
    "response#3": "IVYLTSDPEMKRVDTDIFFSIFADHPKRARAWWAVSVETSDNPFTREYSVENFGTDFLFRVRIRLGFKVSQSIPAYIHQIMNDLSKSGDLPKQTTRYPKLDADPNIGPIRYVLIHKALMPESKVSQKGAISLQIKYAIRHLAGSPVKWFGLAPYNPLIR"
  },
  {
    "reference": "MRSGLHRHPVCDGLLSSPWYFRPAVALSPSILVLLLGEGMSVLKREPPLLHREGPLNDPELLLTPPLPILSIDKIFSLPGHAPTGHSSSSSAEPTTRGRWSFIDCFLRLSEQFMVVGLLQRLSEKLPRVVHRLSEMMKPQVVFVLGGPGAGKGTQCERIVKNYSYTHLSAGDLLREERSRTGSEFGQLIDTYIKEGKIAMEETMKSDEKKFRFLIDGFPRNQDNLQGWNNVMDGKADVKFVLFFDCSSEVCIDRCLERGKSSGRTDDNRESLEKRIQTYLQSTRPIIEQYEQQGKVHKIDASRSVDEVFADVKNILENQG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Plays an important role in de novo pyrimidine nucleotide biosynthesis.2. A protein with nucleoside diphosphate kinase activity, ATP binding functions is required to enable 'de novo' pyrimidine nucleobase biosynthetic process.3. The protein should be designed to catalyze the following reaction a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-   triphosphate + ADP. The designed protein sequence is ",
    "response#1": "MARIPRTYR",
    "response#2": "MSTVRA",
    "response#3": "MPRHLF"
  },
  {
    "reference": "MFLEQRRQQILEHLDKYESMTVESLSALFSVTRETIRSDLNALAAEKLVQRCHGGAMVIRRSLQSKLITDTGDNFEVLLKRLQSQKRKQAGQQKGKNMKGKVCILGSFNVDIVAKVERFPKGGESLLALDSTLGPGGKGSNQATAASRAGAKVHFVSKVGKDQFSQFAYDHLSSSEIHSFTLYQSETEPTGNAIIYVSQEKGENMIAIYSGANKTISEDEVAAIATELEDSEVLLVQLENNFAATLSAMKLAKALGVKVILNPAPFSSHALECLEYVDVITPNETEASQLSGIDVQDLASAKEAAQRIVSQGAQRVIITMGSRGALILDGTQFQHIPAFAALSVDTTGAGDAFNGALAASIAKGQSLAQAATYASAFASLSVEREGAANMPDNAQVIARLAQR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.2. The protein contains novel HTH deoR-type domains that confer a unique function or activity.3. A protein that localizes to cytoplasm and can perform ATP binding, DNA-binding transcription factor activity, metal ion binding, ribokinase activity with high specificity and efficiency.4. The ATP, K(+), substrate binding site should be located in a region of the protein that is accessible to the ligand.5. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in ATP + D-ribose = ADP + D-ribose 5-phosphate + H(+) are properly positioned for optimal catalysis.6. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.7. The protein should be optimized for activity in D-ribose 5- phosphate from beta-D-ribopyranose: step 2/2, Carbohydrate metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "QEICVVGACFTDLIAYVPRMPLPGETLVGTKFSTGFGGKGANQAVQAARLGAKVMMLSKVGADSFGVDTIKNLAEQGIDATHVTQEPGMSSGVAPINVDVSTGQNSIVIVPGALDAFTPVEVEASRERIKKCGLLMCQLEAPLAVTLAALQIGREEGLMTILNTAPAPQSPLPDKIWSLCDIVCANEVELAGLTGLTVDTDADVEVAAAELLRRGTTKLLVTLGDRGCALFEGQPRCLRAVWQPSVRVTPKDTTGAGDSFLGALAYYLTTGCALERALELATLVAAISVTREGTQTAFPSKKD",
    "response#2": "QEICVVGACFTDLIAYVPRMPLPGETLVGTKFSTGFGGKGANQAVQAARLGAKVMMLSKVGADSFGVDTIKNLAEQGIDATHVTQEPGMSSGVAPINVDVSTGQNSIVIVPGALDAFTPVEVEASRERIKKCGLLMCQLEAPLAVTLAALQIGREEGLMTILNTAPAPQSPLPDKIWSLCDIVCANEVELAGLTGLTVDTDADVEVAAAELLRRGTTKLLVTLGDRGCALFEGQPRCLRAVWQPSVRVTPKDTTGAGDSFLGALAYYLTTGCALERALELATLVAAISVTREGTQTAFPL",
    "response#3": "KVCILGSFNVDIVAKVMRFPKGGESIMAQGSAIGPGGKGANQAMAASRAGAQVYFIAKVGTDQFSQFAYDHFTASDIHSFKLYQSDSEPTGNAIIYVSQQNGENMIAIHPGANQTITDDEVAAIASELTTSDVLLVQLENNFSATLNAIKLAHTLGKKVILNPAPYSSEILPYLDYLDVITPNETEASLLSGIDIHDVDDAKEAALKIAALGAKRVIITMGSRGALLLDGKQFQHIPAFPALGVDTTGAGDAFNGAFAAALANGQSLVQAATYASAFASLAVEREGASNCH"
  },
  {
    "reference": "MRVKGIRKNYQHLWKWGIMLLGMLMICSAAENLWVTVYYGVPVWKDANTTLFCASDAKAYDKEAHNVWATHACVPTDPNPHEVELKNVTENFNMWKNNMVEQMHEDIINLWDQSLKPCVKLTPLCVTLNCTNYNNTNTTRNGANNTTNENKGDLEMKEGEIKNCSFNVTSNMRDKVQKEYALFYRLDVEQIGTNNNKSYNSYRLISCNTSRLTQACPKISFEPIPIHYCAPAGFAILKCNNKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEGEVVIRSQNFSDNTKTIIVQLNNTVQINCTRPGNNTRKSISLGPGSAIYATGQIIGDIRQAHCNISRKDWNETLKQVVKKLRGQYGENKTIIFNSSSGGDPEIVMHSFICGGEFFYCNSTPLFNSTWNGTDESNHHGGNDTIITLHCRIKQIINMWQEVGRAMYAPPIRGNISCSSNITGLLLTRDGGDGNGTDNNTEIFRPLGGNMKDNWRSELYKYKVVRIEPLGIAPTKARRRVVQREKRAVGMGALFLGFLGAAGSTMGAASVTLTVQARLLLSGIVQQQNNLLRAIEAQQRLLQLTVWGIKQLQARILAVERYLRDQQLLGIWGCSGKLICTTAVPWNDSWSNKSYADIWQNMTWMQWEREIDNYTGLIYDLLAKSQDQQDKNEKELLELDKWASLWSWFDITHWLWYIKIFIMIVGGLVGLRIVFLILSVVNRVRQGYSPLSFQTHLPAPRGPDRPEGTEEEGGERDSDRSGPLVDGLLALIWV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state2. The designed protein should be expressed specifically in the virion membrane, host cell plasma membrane3. The protein design should emphasize Basic and acidic residues compositional bias.4. The designed protein must have at least one Human immunodeficiency virus 1 envelope glycoprotein Gp120 domain. The designed protein sequence is ",
    "response#1": "VRTHSVLKGIVQQQDNLLRAIQAQQHLLRLSVWGIRQLRARLQALETLIQNQQRLNLWGCKGKLICYTSVKWNTSWSGRYNDDSIWDNLTWQQWDQHINNVSSIIYDEIQAAQDQQEKNVKALLELI",
    "response#2": "MGAASVTLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKTICTTNVPWNSTWSNKSYEEIWNNMTWIEWEREISNYTSKIYDLLMESQSQQERNEKDLLELDKWASLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSIVNRVRQGYSPLSLQTH",
    "response#3": "MGAASVTLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKTICTTNVPWNSTWSNKSYEEIWNNMTWIEWEREISNYTSKIYDLLMESQSQQERNEKDLLELDKWASLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSIVNRVRQGYSPLSLQTR"
  },
  {
    "reference": "MRFFLSFFIIFISSVLFSFGIPNEILNFGSAIAGFSGLVLVYYALLNCGSHKRAAFLYGFFVSFVHLMSSFWLAFFEDFAIFTLGASTLAYFFIAMPFGFLLYHSLQKRENLRPFFFAAIWLLWEFAKSTGFLAYPWGTAPMICFNLKPFIQFVDITGVWGLSFIVPLIAACLGEALQTYAYSANSKAFFKGLTEIKSPLIFTAFLVLIINIYGITILSIEMKPATFLNTVIVQQNTDPWDNSQFEENIKTSQALSRKAIFSANKKPDLIVWSESSLIVPYKDNEDFYGILPYDDPFTRFLADTDTPIIVGSPYIDGKKQYNAAYLLSPEGKILDIYSKIQLVPFAEYIPFIDNPLVVRFFDKLVGFSSGWNPGTEYKVFGIKNSEGKTVNFTVPICFEDAFPAVCLNLHNAGSEVLINITNDSWSKTKSAEYQHFVVAHFRAIELRTTLVRSTNSGYSVVVDPKGKIISDMPLFEAESVYTEVPVYGHKKTFYASYKDWLPIMMFLILIFNIFLEKRRAKTARF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have N-acyltransferase activity within plasma membrane to regulate lipoprotein biosynthetic process.2. For general function, the protein need meet that Catalyzes the phospholipid dependent N-acylation of the N- terminal cysteine of apolipoprotein, the last step in lipoprotein maturation.3. The designed protein have CN hydrolase domains with specific sequence motifs that are crucial for its activity.4. The designed protein must have a active site, Proton acceptor that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "VQPAIDQSRKMLNTDRAEIFAEHLRLSALPPGEGKKRPDIIVWPETSVPFILTQNPDALAEIASTLEDGQVLFTGAVRMEDQGAGRPPRYYNSVYAIDSQGEIIGATDKVHLTPFGEYVPFEGILREFGIDNVIALPGGFSAASSRTPLTLPSGKTFYPLICYEIIFPGEMTPGLQGAAAILNVTNDGWFGDTPGPYQHFLQARVRAVETGVPVIRGANTGISAVIDPYGRIIAGLDYGRVGILDATLSG",
    "response#2": "VQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEINQQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYNKNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIILGEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITAVIGPQGEIQAMIPQFTREVLTTNVTR",
    "response#3": "VRIVQPAIDQSRKMLNTDRAEIFAEHLRLSALPPGEGKKRPDIIVWPETSVPFILTQNPDALAEIASTLEDGQVLFTGAVRMEDQGAGRPPRYYNSVYAIDSQGEIIGATDKVHLTPFGEYVPFEGILREFGIDNVIALPGGFSAASSRTPLTLPSGKTFYPLICYEIIFPGEMTPGLQGAAAILNVTNDGWFGDTPGPYQHFLQARVRAVETGVPVIRGANTGISAVIDPYGRIIAGLDYGRVGILDATLAA"
  },
  {
    "reference": "MFDPVEKVIEAFSRGEIVIVTDDENRENEGDLVVAAEKVTPDAINFMAKYGRGLICVALTRERLEALGIGRMTTRGDGDAFKTAFMESVDAAHGITTGISAHDRARTVAVLVDDKSRPGDLVSPGHTFPLEAKEGGVLRRAGHTEASVDLARLAGLKPAAVICEVLRDDGQMARLNELAELARTHRLLMTSVADLIAYRSRKERLVEFVRATDLPTRYGRFSLRLYRSIAEGDHHVALVMGDVSGMPPPLVRVHSECLTGDVLGSLRCDCGNQLHAAMTMVAREERGVILYMRQEGRGIGLANKIHAYALQDKGLDTVEANLKLGFPPDLREYGVGAQILCDIGLKEIRLLTNNPRKIVGLEGYGLKVVERVPLIVEPTPYSDNYLRTKKEKLGHLL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..2. The protein's Nucleophile; for GTP cyclohydrolase activity must be carefully engineered to ensure that the amino acids involved in GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "VVRAALPTPYGNFELIGFDNDIDHKEHIALVKGEITPDEPVLVRVHSECLTGDVLLSLRCDCGEQLHSAMEMIEKEGKGIIVYMRQEGRGIGLINKLRAYHLQDHGRDTVEANIELGFAPDLRDYGIGAQILVSLGVRKMRMMTNNPKKIIGLEGYGLEIVDRG",
    "response#2": "EAVTRLPTRHGDFRAYGYRSTTDGVEHVALVAGELGDGADVLVRVHSECLTGDVFGSLRCDCGPQLQESLARVQAEGRGVVLYLRGHEGRGIGLLPKLRAYELQESGHDTLDANLALGLPADARDYGAAARILTDLGVRSLRLMTNNPDKTDALLRHGLRVEGRAQMRRR",
    "response#3": "RVAGARLPTPYATFRIFAYQPRDGGQPHLALVLGNVTDGAPVLTRLHSECLTGDVFGSLRCDCGEQLDRAMRAVAAEGRGVILYLRQEGRGIGLCNKLRAYALQDEGLDTVEANERLGFPPDAREYWMAAYMLDDLGVRQVRLLTNNPQKVAALEHYGLTVAERVAI"
  },
  {
    "reference": "MESLPSLLESIYTTWLSLSSTIWAMDMILAFVCGLGLYHLLLPFLESHLSSPPSNIKFTRKPQIQMTWQSQFKKKFRNHCRNDAKAWGECLKKLKEKEKDKLFLEEMSPGHHLNSLGNIFNSSSAKQDSTTLSPFWNLKEKSEEQVATQKLSYPKISEDHFQQKCDQLFWGLPSLHSESLVAAAWIPQTTSTLPSPFFLFNVISSVYPIQLQDKMSPMLPHTHPLSYLDLQSPHLILSPLEFQTPALNLPVLLPSSLPYNSDFGTSYSQSQSKPQYLPTEIKYNKKPSLAKQIENRSTLPLMVQKPQEAYDILAPNPSQDWVVSILPDNFPICRELREKLEQHIQKWLIQHQWNFPHKIQVSEKMKELQNTVIGNCQTRDKPGTLQALGEHSNEGQKRKFQLEKESGKNLGPILGKISKDPIRGLEKTTAIKDLENNLKAHLGTKSGQIDQGLTPLSMRQSWLAVDDSFYEMENNLTSLKSSAKSMCSSEKLAFLKPETRQALEAHIVRFWAKHRWSLPLKILKPVKLFQLSIESLPVVLCAQTSSTTSVHRTRSAAEVVRFLGKPCLRQMIIEDSSPSPQNLLLVSSPSCKRARRRLPFGVDHEPSTALPTKPECGHISEDLTYNFMNTTSQTRTIFKKEIETREVVLLPRRTSDQNLEAYKHQEKVVSEFPHNVETELAGQPQIYTTTVLPPKRSRSMPLPVDTLTSHVLGDIVVADMDNSLVQQRPSTPKHLVSQKSQIKMLAPTYQSEGTKRQSEIKYEDRPKL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be designed to localize specifically to acrosomal lumen, acrosomal membrane in order to perform its role in cell differentiation more effectively.2. The protein must exhibit the following characteristics: May play a role in spermatogenesis..3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "MVQKIKSMREFKELLGAAGNRLVVVEFSAQWCGPCKMIAPAFQAMSLQYRNVMFAQVDVDSSQELTEHCSIQVVPTFQMFKHSRKVTPFSRLKRILCCFRSGPGSKKIFEFQGADIEKLEEKIQELM",
    "response#2": "PRAFRLTKDSVKIVGSTSFPVKAYVMLHQKSPHVLCVTQQLRNAELIDPSFQWYGPKGKVVSVENRTAQITSTGSLVFQNFEESMSGIYTCFLEYKPTVEEIVKRLK",
    "response#3": "PRAFRLTKDSVKIVGSTSFPVKAYVMLHQKSPHVLCVTQQLRNAELIDPSFQWYGPKGKVVSVENRTAQITSTGSLVFQNFEESMSGIYTCFLEYKPTVEEIVKRLR"
  },
  {
    "reference": "MLSFNNTTSASSFQSASSRYLMSSSSSIGPPQQQSAKLFPEHFYPSGLSPRQSEALERLRPNTASRERNIPIQFTGKNPTTNSALEEYKPVPHVQVTSPKSSIVPNFVSEQRGLQPQPTSQAPTNYRQFVAAPRSPRGYGDYPEMTGKASAAGDSEPVQIPIKTQTPITQARAQETKIPTIVSPHPVYYYDNQEQPIQQIREEQPNATMETKVTGQGQPKRVGRWTLAQLRQTDGIIPSQAGWNKGDSQKLMTNFGTPRNTNTRVKSENLQEIPEDIANRTHGEVRLQSGTNKYCSQRGMTGFGSGRDVCREGVRVAQNPADLAELPEEKIRMSEGIVRLQAGTNKYDSQKGMTGFGTGRRETTKMVDSKHPEYDHEKPDQSEIPLQSGTNKFASQKGMTGFGTARRETTKMVDSNHPDYSHECSIDQTTIPSQMGSNQYASQKGMTGFGQPRWEVLDPSISWQNRKSQGMVRLQSGTNRFASQAGMIGFGTCRNTTFEAEGGELPYEAMKVSETIIPSQAGWNKGDSQKKMTSFGAPRDVKGKHLKRIWELEYPEEAEISLDRL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein localizes to the cytoskeleton. The designed protein sequence is ",
    "response#1": "GFDFNIMVVGQSGLGKSTLVNTLFKSQVSRRSTSWSRDEKIPKTVEIKSVSHVIEEGGVKMKLTVVDTPGFGDQINNDNCWEPISKHINEQYEKFLKEEVNIARKKRIPDTRVHCCLYFISPTGHSLRQLDIEFMKHLSRVVNIIPVIAKSDTLTPEEKTEFKQRVRKELEVCGIECYPQKEFDEDMEDKSDNDKIRETMPFAVVGSDKEYQVNGKRVLGRKTAWGVVEVENPNHCEFSLLRDFMIRSHLQDLKEVTHNIHYETYRAKRLNDE",
    "response#2": "AGFDFNIMVVGQSGLGKSTLVNTLFKSQVSRRSTSWSRDEKIPKTVEIKSVSHVIEEGGVKMKLTVVDTPGFGDQINNDNCWEPISKHINEQYEKFLKEEVNIARKKRIPDTRVHCCLYFISPTGHSLRQLDIEFMKHLSRVVNIIPVIAKSDTLTPEEKTEFKQRVRKELEVCGIECYPQKEFDEDMEDKSDNDKIRETMPFAVVGSDKEYQVNGKRVLGRKTAWGVVEVENPNHCEFSLLRDFMIRSHLQDLKEVTHNIHYETYRAKRLNQ",
    "response#3": "QRCHDLEQQVSLLMEEKKNMMGENRTLKEQQEQSGMGTPQLFNKKLLLLQSQIEQLQEENYRLESSRDDYRQRCQELDRDVQELQQRNQDLTGLAHESQALRDEMDVLRHSSDRVGKLESLVDSYKKKLEDLGDLRRQVKLLEERNTMYMQRTFQLEEDLHKANASRGQVESLSRQVQELHKKHSTESLRAEKWQFEFQTLKEKFEALQKERERLIAERDSLRETNDELRCSHLQQTCLGQADLLLSGSSPPLENLAAEIVPAELRETVIRLQQENKMLCAQEASYHERLCDLQNFLEESNRSKNRLESESRLQQQQIKGLKAQVEELQKQLREQGNRAEDSSQLKRKLEEHLEMLHDAHSELQKKREYIETLEPKADLNMSRKVDELQQILHQKEEDMRAMEERYKRYVDKARTVIKSLDPKQQNYIPPEIQALKNQLQEKDTRIRHLETDYEKTKVQRDQEEKLIISAWYNMGMALHQKSTDEKTQPPNGAQSFLAQQRLATNARRGR"
  },
  {
    "reference": "MDLHQLYLDRLRQRDRIEGNFCYLFEAAVVLETAQPPQDSRDLVAKSLREELHAHEQEIHKLKDIVHLRSKDAEKLNDEIISLNIENSLLQDKLTALQAEYDKLIQRWLAKAQSEADAMNQGLA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Stabilizes the ATG5-ATG12 conjugate which is necessary for autophagy.2. The protein can be expressed in phagophore and perform identical protein binding, Atg8 ligase activity without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "EAMKKYVTSRFWFTYRRDFSPIGGTGPSTDQGWGCMLRCAQMLLGEVLLRRHIGRHFEWDIEKTSEIYEKILQMFFDEKDALYSIHQIAQMGVTEGKEVSKWFGPNTAAQVMKKLTIFDDWSNIAVHVALDNILVKEDAITMATSYPSEDAVKLIMENGLVDKNRLSLSPGNIIPEWRPLLLMIPLRLGLTTINPCYLSAIQEFFKIPQCVGIIGGRPNHALYFVGMSGSKLFYLDPHYCRPKTESTAKMYAEKDSTATTDDVGFSHLEELVPLPSQTADVYTKMDDSTYHCQMMLWIEYENVDPSLALAMFCETRDEFENLCRIS",
    "response#2": "EEAHEDIIKKLCLTYRYGFERIPRAVNGPSPLSFMQSVIFSKSLLYNLQNFNNFIEKENFTTDVGWGCMIRTSQSLLANTFVRLLDKQSDIIALFNDTYLAPFSLHNFIRVASSSPLKVKPGEWFGPNAASLSIKRLCDGYYDNSTSETILPRINVLISESTDLYDSQIAQLLEPSTETKGLLVLLPVRLGIDSINSYYFSSLLHLLSLEQSVGIAGGKPSSSFYFFGYQDNSLIYMDPHSAQIFSSDIDMSTYYATRYQRVDIGKLDPSMLIGVFIRDLTSYENFKG",
    "response#3": "EEAHEDIIKKLCLTYRYGFERIPRAVNGPSPLSFMQSVIFSKSLLYNLQNFNNFIEKENFTTDVGWGCMIRTSQSLLANTFVRLLDKQSDIIALFNDTYLAPFSLHNFIRVASSSPLKVKPGEWFGPNAASLSIKRLCDGYYDNSTSETILPRINVLISESTDLYDSQIAQLLEPSTETKGLLVLLPVRLGIDSINSYYFSSLLHLLSLEQSVGIAGGKPSSSFYFFGYQDNSLIYMDPHSAQIFSSDIDMSTYYATRYQRVDIGKLDPSMLIGVFIRDLTSYENFKIV"
  },
  {
    "reference": "MRLSQLLLDMDYTVLQGEQDREISTLVYDSRKVEQDSVFVCISGAVRDGHDFAPEVAAKGAAVLIVEKEVTVPDEVTVIRVADSRLALAQLSAAYFDHPAKELTTIGITGTKGKTTATYMVRSILEASGFKTGLIGTIETIIGDTVIPSVNTTPESYVVQESFRKMVDAGCKCVVMEVSSQALMLHRVSGFVFDYGIFTNLEPDHIGPNEHKDLADYIHCKSLLFKQCKHGIFNADAHHLDEVLEGHTCEVETYGFGEDADLRASNVHLMDKGGTLGVAYHMSGALDMDVEIDIPGTFSVYNSMTAIAICRHFGVQKETILNSLSQIRVKGRVEILPVSSKFTLMIDYAHNAMSLESLLSTLREYHPKRLVCLFGCGGNRSRERRFEMGEVSSKMADLTIVTSDNPRNEQPEAIIADIVTGVEKADGKYITIIDRAEAIRYAIEHAQEGDVIVLAGKGHEDYQEICGKKYHMDERELVADVVKDGNFLQ",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.2. A protein should be able to manipulate the activity of enzymes in the peptidoglycan biosynthesis pathway, either by activating or inhibiting their function.3. The designed protein must be able to regulate cell cycle, cell wall organization, peptidoglycan biosynthetic process.4. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan. The designed protein sequence is ",
    "response#1": "GRMEKIEIDNNDVSRSLPIVIIDYAHTPDGLKKVLQSIKKLCKGKLITVFGCGGDRDRSKRPLMGSIAEEFSDHVFITSDNPRSEEPQQIVNDILMGIEKREKITFDIDRFKAINESIKFANKEDIVLIAGKV",
    "response#2": "ITGTNGKTTTAFIAHAILKNVQHRAGLLGTIKVDNGLEEREATHTTPDPLQLQQVFSEMVENGCRAVAMEVSSHALHQKRTAGTSFDVGVFTNLSQDHLDYHVSMEAYFNAKKLMFESMVASESKKRPTAVINIDERHGELLVRDYKDDLRILTFGFSVHADFRAVNVKQGMSGTQFELQYKGKDYLVKTPYFGLYNVSNALAALTGTNGKTSVVSYCRQLYTLLGVPSCSIGTIGVECASGLDLSVQDILDKSPALTTLDPITFKHILHKLTENNTKYVAFEASSHGLDQQRLCGVKVNAACFTSFSQDHLDYHQNMDKYLLAKLKLFTDNLLPTGTAVLNSEIAQLDYIKHYLQQRNIKFLTVGMNGDLKIIDSTIPGHKVCDYKGQKYNFTTDIVGSFQASNLLIAVMGRMEAFSAAGKPVVIVDYAHTPDALQQVLTATRKHCKGQLWVVFGCGGDRDRGKRPQMGRVAAELADRVIVTDDNPRTEEPAHIVNDILTGMPNRRHVDVQFGRREALQKALQQAASDDVIILAGKG",
    "response#3": "ITGTNGKTTTAFIAHAILKNVQHRAGLLGTIKVDNGLEEREATHTTPDPLQLQQVFSEMVENGCRAVAMEVSSHALHQKRTAGTSFDVGVFTNLSQDHLDYHVSMEAYFNAKKLMFESMVASESKKRPTAVINIDERHGELLVRDYKDDLRILTFGFSVHADFRAVNVKQGMSGTQFELQYKGKDYLVKTPYFGLYNVSNALAALLVLEALGY"
  },
  {
    "reference": "MITENQYFDGNVKSLGYETPEGKSTIGIINPGEYEFGTANKEIMHVIEGSLTILLHGETQWQVIESGSSFEVSSNSSFKVKTEVQTAYLCQYR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a fast turnover rate for phosphate + xanthosine = alpha-D-ribose 1-phosphate +   xanthine.2. For general function, the protein need meet that Catalyzes the phosphorolysis of diverse nucleosides, yielding D-ribose 1-phosphate and the respective free bases; Also catalyzes the reverse reactions.3. The designed protein must be optimized for pyrimidine-nucleoside phosphorylase activity, uridine phosphorylase activity, thymidine phosphorylase activity, guanosine phosphorylase activity. The designed protein sequence is ",
    "response#1": "MKEVIVTGFEPFGGERVNPSWEAVKQLDGKTLDGAKVVKLQVPTVFAKSVDALNKELEKTKPALVICIGLXVXIXIXRXRXNXDDXIDDXGXKDXSGILXNGEPIVEGXPAYFSTLPIKAIVKXLRXEGYDARVSXTAGTYVCNHXFYGLMXHLDKXKXETRAGFMHIPYXIDXVAXKPXTPNTXMDXIIKGXQXAIKVALKXHXEXIAXTXNXIXNXXNXNXN",
    "response#2": "MERFLTGAEER",
    "response#3": "MSILSGRSIVVLTGAGISAESGVPTFRGAGGLWRDHRPEELATPEAFARDPA"
  },
  {
    "reference": "MATKRALVILAKGAEEMETVIPTDLMRRAGMAVTVAGLAGKDPVQCSRDVFICPDKSLEEARKEGPFDVVVLPGGNLGAQNLAESPVVKEVLKDQDGRKGLIAAICAGPTALLAHGIGFGSKVTTHPLAKEKMMAGDHYQYSENRVEKDGHILTSRGPGTSFEFGLAIVEAVLGKEVATQVKGPLVLRD",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein have enhanced an N(2)-(1-hydroxy-2-oxopropyl)-guanosine in RNA + H2O = a   guanosine in RNA + H(+) + lactate.2. The protein should have a specific function in autophagy, single fertilization and should be localized to membrane raft in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "FVPFAVCVVSRFPYYNSLKDCLSCLLALLKPCKDFEVDSHIKDFAAKLSLIPSPPPGPLHLVFNMKSLQIVLPARADPESPILDLDLHLPLLCFRPEKVLQILTCILTEQRIVFFSSDWALLTLVTECFMAYLYPLQWQHPFVPILSDQMLDFVMAPTSFLMGCHLDHFEEVSKEADGLVLINIDHQ",
    "response#2": "FVPFAVCVVSRFPYYNSLKDCLSCLLALLKPCKDFEVDSHIKDFAAKLSLIPSPPPGPLHLVFNMKSLQIVLPARADPESPILDLDLHLPLLCFRPEKVLQILTCILTEQRIVFFSSDWALLTLVTECFMAYLYPLQWQHPFVPILSDQMLDFVMAPTSFLMGCHLDHFEEVSKEADGLVLINIDHS",
    "response#3": "LLDNADSVEPSFVMEKIGRQPKLQLAYLTKLMSRNEGTEFADKAVQLYAEYDQKKLLPFLRKNANYNVNKARKLCSDKGYIEETIYLLAKSGNHYDAVKMMVREYRNMEKVIDYCKDQNDPDLWIHLLGVVAEFPAHFSR"
  },
  {
    "reference": "MANEVYANNMEISCKAANGKSIAAFPDVCFTPPQAPPTPLGVPIPYPNTGLSKDTTKGTRTIRITRKEVMLKNKSYYKTSYGDEPGRAPKKGIVTSKIKGKVYFTSWSMNVKFESKNVVRHLDLTTHNHASFPGNTPVWPYLDQATVDAGGGPCSNEVKKEKKDCADFKPHGSKDACAGLGAGKPSGKKTSNEADRLADKVAARKCLTARRCALQPYKPNSCCPQQTAHHLIEASALHDKGRGGKGSVPLKGISNYSENKAPCVCAEGVNQNVGTHGLMHTFQSAAAAKSRSGTLQLSNGSSISAKKTTYGTAKRQSMAAMGKVFPQSKCSKECLSAQLDNYHKQCGINARTPIKAVETGQTDVTAATQAIKTRNARLGATRSRVR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a low Km value for Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-   phosphooligonucleotide end-products..2. The designed protein must be able to regulate protein secretion by the type VI secretion system.3. The protein must exhibit the following characteristics: The activity is initially neutralized by a cognate immunity protein Tsi7.. The designed protein sequence is ",
    "response#1": "NLMGNKEKADQQKAVSDIVALENALDMYKLDNNRYPTTEQGLDALVNKPTAAPEPRSYRDGGYIKRLPQDPWGNPYQMLSPGQFGKIDIFSMGLDGEAGTDDDIGNS",
    "response#2": "NLMGNKEKADQQKAVSDIVALENALDMYKLDNNRYPTTEQGLDALVNKPTAAPEPRSYRDGGYIKRLPQDPWGNPYQMLSPGQFGKIDIFSMGLDGEAGTDDDIGNC",
    "response#3": "NLMGNKEKADQQKAVSDIVALENALDMYKLDNNRYPTTEQGLDALVNKPTAAPEPRSYRDGGYIKRLPQDPWGNPYQMLSPGQFGKIDIFSMGLDGEAGTDDDIGNL"
  },
  {
    "reference": "MSELRNKRVLLGISGGIAAYKSAVIARHLVRAGAEVRTVMTEGARAFLQPLSLQALTGHPVHTELLDAEAEAGMGHIELARWAELVLIAPATANTMARLANGFASDLLSTIYLATDAPVIIAPAMNRLMWQHAATQSNRQRLIEHGVMVIGPGSGLQACGETGDGRMLEPEQIVASLELHLERSPFRGSAERAIDPEADTRIGTQHLPRKEQAPELGVPAMLRDPRLKGRDILITAGPTREPIDPVRYISNRSSGKMGYAIARMARAHGASVTLVSGPTRLDPPDGVKRVDVTTAQEMYDAVLERAAQADIFISVAAVSDFRVESIADRKIKKTEGNDELNLMLVRNPDILATVAALPKRPFCVGFAAETHDVEAHARGKLERKKLDMIAANHVARADNPVFASDRNALDVYWASGKAAIGPGHKLDVAAELVELVAERFAASRSAAGT",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein with metal ion binding, phosphopantothenoylcysteine decarboxylase activity functions is required to enable coenzyme A biosynthetic process, pantothenate catabolic process.2. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.3. The protein design should consider the endogenous proteins and enzymes present in the CoA from (R)- pantothenate: step 3/5 pathway to avoid interference with native metabolic processes.4. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A5. The designed protein should be able to compete with endogenous proteins or inhibitors for CTP binding.6. The desired protein should be able to perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2 The designed protein sequence is ",
    "response#1": "MNIVLGVSGGIAAYKACILLRLLKESGHDVTVIPTEAALKFVGEATWEALSGHQVASGVFSDVSEVKHVRLGRHADLVVVAPATADLLARAAHGRADDLLTSTLLTATCPVVFAPAMHTEMWEHAATRANVATLRERGAHVVEPDSGRLTGADTGPGRLPEPESLARALVAATSHGLLSGRHILVTAGPTHEPIDPVRYIANRSSGRQGFAIAAALADLGARVSLIAGPVALPTPAGVTRIDVESALDMAAAVDAALPADAAVMVAAVADWRVEAAPQKVKKGDAPPALHLVENPDILATLARAPNRPPLVVGFAAETERVVDHAAAKRLRKGADWIVANDVSQDVFGSDGNAVHLVSTDGSEHHVDGDLPTKSELARVLVDHVAARLG",
    "response#2": "MNIVLGVSGGIAAYKACILLRLLKESGHDVTVIPTEAALKFVGEATWEALSGHQVASGVFSDVSEVKHVRLGRHADLVVVAPATADLLARAAHGRADDLLTSTLLTATCPVVFAPAMHTEMWEHAATRANVATLRERGAHVVEPDSGRLTGADTGPGRLPEPESLLAAAGALLGGGLAGRKVLVTAGGTREPIDPVRFIGNHSSGRQGIALAAAAAARGADVHLIAAHLDAPLPAGISVERVSTTGELYDATVKAAAEADAAVLAAAPADFTPKTSSDRKLKRKGDLELALTATPDIAAAVGRAKRDGQVLVVFAAETHDGPEHAKAKREAKGADLVVLNDVSGGAVFGAAENSVTVLAAGGGIVLAGKIIAVSAGGTREPLDPVRYLGNRSSGRQGIALAKAALAAGATVRFIAAHMDLPAPAGVELSTASTAEQLQQAVTTAARGADVLIMSAAVADFRPAEYLDSKIKKSDDRADPVITLVRNPDILAMLIAERAAEGPAGDLPPLVVGFAAETGDADSSVLEYGAAKLARKGCEMLVVNEVGENLTFGTDKNTVTILAADGSETVVPKGSKQAVADAVWDLVAARLPA",
    "response#3": "MNIVLGVSGGIAAYKACILLRLLKESGHDVTVIPTEAALKFVGEATWEALSGHQVASGVFSDVSEVKHVRLGRHADLVVVAPATADLLARAAHGRADDLLTSTLLTATCPVVFAPAMHTEMWEHAATRANVATLRERGAHVVEPDSGRLTGADTGPGRLPEPESLLAAAEALFGGPLAGRKVLVTAGGTREPIDPVRFIGNHSSGRQGIALAAAAAARGADVHLIAAHLDAPLPAGISVERVSTTGELYDATVKAAAEADAAVLAAAPADFTPKTSSDRKLKRKGDLELALTATPDIAAAVGRAKRDGQVLVVFAAETHDGPEHAKAKREAKGADLVVLNDVSGGAVFGAAENSVTVLGADGSEAEVAQGSKSALAHAVWDTVLAALADDPPPDEG"
  },
  {
    "reference": "MDTSRLTLDHFLSRFQLLRPQITHETLNQRQAAVLIPVVRRPQPGLLLTQRAIHLRKHAGQVAFPGGAVDSTDASLIAAALREAQEEVAIPPQAVEVIGVLPPVDSVTGFQVTPVVGIIPPNLPWRASEDEVSAVFEMPLAQALQLGRYHPLDVYRRGNSHRVWLSWYEHYFVWGMTANILRELALQIGVKP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a high affinity for Mg(2+).2. The protein contains novel Nudix hydrolase domains that confer a unique function or activity.3. The designed protein should have magnesium ion binding, manganese ion binding, CoA pyrophosphatase activity to facilitate nucleoside diphosphate metabolic process.4. The presence of Mn(2+), Mg(2+) is crucial for the protein to function optimally under specific conditions.5. For general function, the protein need meet that Probably mediates the hydrolysis of some nucleoside diphosphate derivatives. The designed protein sequence is ",
    "response#1": "KTHDSVTILMFNSSRRSLVLVKQFRPAVYAGEVERLFPGSLAAAEQDRPQALQAALPGSAGVTYELCAGLLDQPGLSLEEVACKEAWEECGYRLAPSDLRRVTSYKSGVGLTGSSQTMFYAEVTDAQRGSPGGGLAEEGELIEVVHLPLDGARTFADDPDVPKTLGVIS",
    "response#2": "KTHDSVTILMFNSSRRSLVLVKQFRPAVYAGEVERLFPGSLAAAEQDRPQALQAALPGSAGVTYELCAGLLDQPGLSLEEVACKEAWEECGYRLAPSDLRRVTSYKSGVGLTGSSQTMFYAEVTDAQRGSPGGGLAEEGELIEVVHLPLDGARTFADDPDVPKTLGVIV",
    "response#3": "TLVLVLQSQRVLLGMKKRGFGAGRWNGFGGKVQDGETIEDGAKRELQEESGLTVEALHKVGHIVFEFVGEPELMDVHIFCTDSVQGTPVESDEMRPQWFQLDQIPFADMWPDDSYWFPDNEH"
  },
  {
    "reference": "MATGPRYRVPFRRRREGRTNYHVRYRLILSRKPRVVVRKGNANITIQLIIAELKGDRTLLTVSSKELRNYGFQHSTGNVPAAYLTGLLFGKKMLALGYNEGILDIGLHSNSRGSRVYAALKGVVDAGVDVPHSPEVFPDDSRIRGEVIRDYTGVDVVSQFEAAREKILG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.2. The designed protein should be involved in translation by localizing to ribonucleoprotein complex and exhibiting structural constituent of ribosome, 5S rRNA binding. The designed protein sequence is ",
    "response#1": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTS",
    "response#2": "MTSAK",
    "response#3": "ITLDLPLVFTGEAVGVKMDGGLLEKVLTQLTVNTPPTDVPEAIEVDISTMQVGDMLYVKDLVLPPGIEVVNTPDLVVAHLTN"
  },
  {
    "reference": "MASSCSRRLLAAALLPWCCAAWALGHLDPPSPPPLVIWHGMGDSCCNPMSMGVIKKMVEKEIPGIYVLSLEIGKNMMEDVENSFFLNVNVQVNMVCQILEKDPKLQQGYNAIGFSQGGQFLRAVAQRCPTPPMMTLISVGGQHQGVFGLPRCPGESSHICDFIRKSLNAGAYSKLVQERLVQAQYWHDPIKESVYRNYSIFLADINQERCVNESYKKNLMALKKFVMVKFFNDSIVDPVDSEWFGFYRSGQAKETIPLQESTLYTEDRLGLKKMDKAGKLVFLAKEGDHLQISKEWFTAHIIPFLK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The ideal protein would be one that plays a critical role in pinocytosis through sulfatide binding, lysophosphatidic acid binding within nucleus. The designed protein sequence is ",
    "response#1": "ADSVTLAIVPEREGMFLFRHVNYSISSVSGTDRITVIRRYSDFSWLQDYLLKKYCFRQVPLLPPKRLAVNGHYLSSDNYFLERRRRGLTRFINQVLRHPVLGQDEAVRTFVTLRNDIL",
    "response#2": "SVTLAIVPEREGMFLFRHVNYSISSVSGTDRITVIRRYSDFSWLQDYLLKKYCFRQVPLLPPKRLAVNGHYLSSDNYFLERRRRGLTRFINQVLRHPVLGQDEAVRTFVTLF",
    "response#3": "GTDRITVIRRYSDFSWLQDYLLKKYCFRQVPLLPPKRLAVNGHYLSSDNYFLERRRRGLTRFINQVLRHPVLGQDEAVRTFVL"
  },
  {
    "reference": "MTGELWLTLVDTADIVGFSMTFCVNIVLLFLLKNRGKNLGTYKHLMAFFSVFSIFYAIIESILRPIMHIENATFFLISRKRFNYSTRLGKINSAFYCACFATSFVVSGVHFVYRFFASCKPHLLRSFNMPYLLLWPLGCSIPVMMWASVSYFLYPDTAFTEAAVTNVLNTHYHSIKKDNVSYIAYVYYQYDENGVRYVYLKNLLGCFVHYFVMSATFVVMFICGYLTWKTMRKHKTASDRTRQLQKQLFKALVLQTLIPTIFMYAPTGVMFIAPFFSINLNANANFIVFCSFLYPGLDPLILILIIRDFRQTVFKFFCLRKKNSVDESRSTTRANMSQVATH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should contain Helical; Name=6, Helical; Name=3, Helical; Name=1, Helical; Name=4, Helical; Name=5 transmembrane regions to facilitate transport across the membrane.2. The Extracellular, Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.3. A protein that contributes to positive chemotaxis, olfactory behavior by functioning in ciliary membrane, periciliary membrane compartment with G protein-coupled olfactory receptor activity. The designed protein sequence is ",
    "response#1": "GNTTIILVSRLEPKLHMPMYFFLSHLSFLYRCFTSSVIPQLLVNLWEPMKTIAYGGCLVHLYNSHALGSTECVLPAVMSCDRYVAVCRPLHYTVLMHIHLCMALASMAWLSGIATTLVQSTLTLQLPFCGHRQVDHFICEVPVLIKLACVGTTFNEAELFVASILFLIVPVSFILVSSGYIAHAVLRIKSATRRQKAFGTCFSHLTVVTIFYGTIIFMYLQPAKSRSRDQGKFVSLFYTVVTRMLNPLISALNY",
    "response#2": "GNTTIILVSRLEPKLHMPMYFFLSHLSFLYRCFTSSVIPQLLVNLWEPMKTIAYGGCLVHLYNSHALGSTECVLPAVMSCDRYVAVCRPLHYTVLMHIHLCMALASMAWLSGIATTLVQSTLTLQLPFCGHRQVDHFICEVPVLIKLACVGTTFNEAELFVASILFLIVPVSFILVSSGYIAHAVLRIKSATRRQKAFGTCFSHLTVVTIFYGTIIFMYLQPAKSRSRDQGKFVSLFYTVVTRMLNPLIS",
    "response#3": "GNLLIVLAICSDVHLHNPMYFLLANLSLVDIFFSSVTIPKMLANHLLGSKSISFGGCLTQMYFMIALGNTDSYILAAMAYDRAVAISRPLHYTTIMSPRSCIWLIAGSWVIGNANALPHTLLTASLSFCGNQEVANFYCDITPLLKLSCSDIHFHVKMMYLGVGIFSVPLLCIIVSYIRVFSTVFQVPSTKGVLKAFSTCGSHLTVVSLYYGTVMGMYFRPLTNYSLKDAVITVMYTAVTPMLNPFIYSLRNSEMKR"
  },
  {
    "reference": "MSVHMGTIEQALADLVAGKAIVVVDDEDRENEGDLIFAAEKATPELVGFMVRYTSGYICVPMSEDSCTRLDLPPMFHTNQDKHSTAYTVSVDARTDVTTGISATDRATTIRKLADPDSVAGDFTRPGHVVPLRAREGGVLRRAGHTEAAVDLTRLAGLQPVGAICEIVSQKDVGDMARVDELEVFAAEHDLTMISIADLIAFRRRSEKHVSRIAAARIPTKYGDFSAVGYDTEFDDVEHVALVRGDIGNGEDVLVRVHSECLTGDVFGSLRCDCGPQLDASLAAVATEGRGVVLYVRGHEGRGIGLLHKLQAYQLQDQGSDTVDANLELGLPADARDYGTGAQILVDLGVKSMRLLTNNPDKRAGLEGYGLTITGRVPLPLSVNAENFRYLATKRDRMGHVLDGLPSEFKA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must be optimized for GTP cyclohydrolase II activity. The designed protein sequence is ",
    "response#1": "RGEEVDLPTQYGHFRLIPFRELASGLEHVALIKGDLSGDEPVLVRMHSSCITGDTFASRRCECGEQLHRAMEMIEKEGRGAIVYLSQEGRGIGLMDKIRAYKLQEEGLDTVDANLHLGHRADERDYGVGANILHELGIRSMRLLTNNPMKRRALNGYGLKVVETVG",
    "response#2": "EVSVKLPTEWGEFEMTAYTQLNTGDNHIALTKGTWEPGEPILVRVHSSCVTGDIFGSCRCDCGPQLHAAMQMIEKEGKGVIVYMNQEGRGIGLVNKLKAYNLQENGFDTVEANIKLGFKMDQRDYGVGAQILRSLGISKMRLMSNNPKKRAGLIGYGLEVVENVI",
    "response#3": "RGEEVDLPTQYGHFRLIPFRELASGLEHVALIKGDLSGDEPVLVRMHSSCITGDTFASRRCECGEQLHRAMEMIEKEGRGAIVYLSQEGRGIGLMDKIRAYKLQEEGLDTVDANLHLGHRADERDYGVGANILHELGIRSMRLLTNNPMKRRALNGYGLKVVETVG"
  },
  {
    "reference": "MPTSSRSTNLYTVEQVRRIDQAAIREGGLPGTELMQRAATAAFAALRRIWPQARTLTVIAGSGNNGGDAFLLARLAREAQWQVQLVALGERSGGDAAAARDAWIREGGRITVADMQTRLPDADVLVDGLFGTGIARAPEGVAAHLIEAMNAFRGGRLALDVPSGLDADNGTAPGPVLRADATVSFVGWKRGLFTADGVDCCGQLELDLLGIPDPVCAGIGPDCELLEAGFANVLPPRIRNVNKGRFGHVLIIGGDIGMPGSVRLAGEAALRVGAGLVSIATRAQHIGAVVGDRPELMAMAADGPQSTEAMLARASVLAVGPGLGQGAWGHALWDKALRAGLPLVLDADGLNLLARQPLPLPATTVLTPHPGEAARLLGCDTAAIQADRFAAARALASRYACVVVLKGAGSLVAAADGRIALCPFGNPGMASAGMGDVLTGVVAGLLAQGLGAWDAACLGTLVHARAGDRAAGPRPRGMIASDLFEALRDVVNGSPS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be optimized for NADPHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADHX epimerase activity, metal ion binding.2. The designed protein must have at least one YjeF N-terminal domain.3. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.4. The protein should have a high affinity for (6S)-NADPHX, AMP, K(+). The designed protein sequence is ",
    "response#1": "MRKMDQTAVETYEIPGVVLMENAGREVALAVKNMWDCQREDKRPANMTKVVLFCGKGNNGGDGFVAARHLANMGFDTNVFIIANPSSITGDAAINLKIAKNMGLKIKVIDEETDLNAVMKMTSNASILVDAILGTGLKGDVRGIARNVIEMINKSEIPVIAVDIPSGICGETGKVLGNAVKAEQTVTMAAPKIGLLLYPGVEYVGKLIVADIGMPINLIEDESVAALLPERLPDSHKGTFGTALIAAGSVNYTGAAVLAGEAAYRSGAGLVTLAVPAPLHGVLAGQFPEATWVLLPHEMGVISEDASAVLKKHFERATALLVGPGFGMDATTKIFIENLLAGKAVQKKGAPRIGFIHQEEGEPDEALTQLPPMIVDADGLKLLAQIKDWHAKLPAASILTPHPGEMSVLTGLSIEEIQEDRQTVAVKYAKEWGHVVVLKGAFTVIASPDGRSTLIPVASPALARAGTGDVLAGLIVGLRAQGLEPFDAAVAGAWIHAQAGLYAADDLGTAASVLAGDVLDSVSDVLN",
    "response#2": "LLIAGSEEKMGAAIMATGAALRSGTGLVTAQVPASGATALNICYPEAMLLMRSNTIDNINHYTAIGIGPGLGVNTDSQQILFNVISHYKQPMVIDADALSILAENADWLRLLPKGSILTPHPKEFDRVFGKCVNDFERLHKALALTLQYPLTIVLKGHYTLVAQQGKGFFNTTGNAGLAKGGSGDVLTGIITALLAQKYEPCIAARLGVYLHGTAADLALETEAEESLVATQVLG",
    "response#3": "MYIPRLKFTHKGTYGHVLAIGGSKGKIGAIRLASEAALTTGAGRVTAQIPKCGYLPLQTSVPEVMVEADDQEDFLTNFFVSDSHSVVCLGMGMGQEDKTVSAFNRFIKQYTGPLVIDADGLNILAKHPELKGHLPKDAILTPHPKELKRLLGDWKDDFEKLELVKAFSIKYKCIVILKGAHTIVVQGGKGFINSTGNPGMATAGTGDVLAGMVASFLAQGYGPLQASIFAVYLHGLAGDLAIAENGYEAMTASKLIDFIPY"
  },
  {
    "reference": "MALIALGVTGGIGAYKAVEIARGLQKNGHDVVAVMTRAARQFVGPLTFEAITRHEVITDQWAPGANADIEHIALASTIDLLLVAPATANTVAKFANGLADDFLSALYIATRAPVLIAPAMNTNMFEHPAVARNLETLIARGVHVVDPGSGYLACGWIGKGRLAEPEDVVAAAEQVLKATPVSAKPLGEGGLTGRRLLVTAGPTYEDIDPVRYVGNRSSGRMGFALATEALRRGAAVTLVTGPSHLPAPHGADVVRVRSAAEMHGAVMTRAAAQDVVIMAAAVADYTPVEKAPQKVKKTDAGLTIALQRTKDILGDVGRLPSRAHGRPVLVGFAAETEDVVAHARAKLDKKQADLIVANDVSRTDAGFDVDRNAVTLVSAAGAEDVPLQSKSTVAARILDRVEQILASPASNVPAGA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must have a Proton donor that is structurally stable and resistant to denaturation.2. The protein must exhibit phosphopantothenate--cysteine ligase activity.3. The designed protein should interact with enzymes and substrates within the coenzyme A biosynthesis pathway to enhance or regulate its activity.4. The designed protein should contain one or more DNA/pantothenate metabolism flavoprotein C-terminal domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MKVALGVSGGIAAYKAAEIVRLLQERGIRVKVIMTHAAQEFVRPLTFAALSGEKVITDMFAAGNEQPNIDSAVEHIAVAQSIDALLVAPATADVLARFAQGISSDFLSTLYLATTAPVVVAPAMNVNMWNHPATQANLDVLKKRGVRVVDPGEGYLACGMTGPGRLAENELIVAATLEEALAA",
    "response#2": "KRILLGITGGVAAYKAAELTRLLVKAGADVRVVMSTAATQFVTPLTFQALSGKPVLTQLWETGVDNGMAHIEFTRAVDAVLIAPASADFIAKLVHGLADDLLSTLCLARACPLLVAPAMNRQMWENPATQRNIHQLQADGVTLLGPTAGEQACGETGPGRMLEPAELLA",
    "response#3": "LAGRRILVTSGPTHEPIDPVRYIANRSSGAQGTALANALRDLGAEVIFVTGPADAPRPQGVQVVEVESAREMLAAVEAALPPQGPAADAAVFAAAVADWRVASASTSKIKKQAGAPAPALEFAENPDILKTVSRWSEGRPPLVIGFAAETDDVEAHASAKRLRKGCDWIVANDVSPATGIMGGTENAVILVCIGVSGGIAAYKALDVVSALRKKDIDVRVIMTESATKFVTPLPFQSLSQNMVTTDMFEEPKAFEIQHISLAKRADIFLVAPATANIIGKVANGIADDMLSTTIMATRAKVIFAPAMNTNMYENKIVQSNIEKLKGLGYEFIEPASGRLACGDEGKGKLADVNTIVERVLDAIEALG"
  },
  {
    "reference": "MKIFKKVFLFILMITISFLNIVKSTEIDENNNNNNNNNSTSSSEKIIKGKEDEEELKNAFSSSVIPPTDHNSELRRKKVIASTITLFAFIILVLIVSIVVRHVLKKKKRTKNNFIFLDDQSLNDCNIELNVSSEYFCDENKLLTTHE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization.2. The designed protein should have a specific function within the membrane.3. The Extracellular topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells. The designed protein sequence is ",
    "response#1": "PQLSLSSPNPSDIRVAWLPLPPSLSNGQVVKYKIEYGLGKEDQIFSTEVRGNETQLMLNSLQPNKVYRVRISAGTAAGFGAPSQWMCG",
    "response#2": "MQAMRFAVEEINNDSSLLPDVRLGYEMVDVCYVSNNVQPVLYFLAQEDNLLPIQEDYSNYVPRVVAVIGPENSESVTTVANFLSLFLLPQITYSAISDQLRDKQRFPALLRTTPSAKHHIEAMVQLMLHFRWNWISVLVSSDTYGRDNGQLLGDRLAGGDICIAFQETLPTLQPNQDMMPEDRQRLVSIVEKLQQSTARVVVVFSPDLTLYDFFREVLRQNFTGAVWIASESWAIDPVLHNLTGLHRTGTFLGITLQNVPIPGFNEFRVRGPQAGPTHQRSTCNQECDTCLNSTLSFNTILRLSGERVVYSVYSAVYAVAHALHSLLGCDHSACTKRGVYPWQLLEEIWKVNFSLLDHQIFFDPQGDVALHLEIVQWQWR",
    "response#3": "MQAMRFAVEEINNDSSLLPDVRLGYEMVDVCYVSNNVQPVLYFLAQEDNLLPIQEDYSNYVPRVVAVIGPENSESVTTVANFLSLFLLPQITYSAISDQLRDKQRFPALLRTTPSAKHHIEAMVQLMLHFRWNWISVLVSSDTYGRDNGQLLGDRLAGGDICIAFQETLPTLQPNQDMMPEDRQRLVSIVEKLQQSTARVVVVFSPDLTLYDFFREVLRQNFTGAVWIASESWAIDPVLHNLTGLHRTGTFLGITLQNVPIPGFNEFRVRGPQAGPTHQRSTCNQECDTCLNSTLSFNTILRLSGERVVYSVYSAVYAVAHALHSLLGCDHSACTKRGVYPWQLLEEIWKVNFSLLDHQIFFDPQGDVALHLEIVQWQWR"
  },
  {
    "reference": "MREKLKDIRTLSIDSLRDFFIQNKFQSYRGDQVYEWIWKKGIHDFNQMSNLSKELRNLLINNFKINHVDIDFFKKSNDGTIKNSVKLHDGLFVESVLIPSKNRSTACVSSQVGCSLNCKFCATSKIKRLRNLNMDEIFDQVILMDKQSRTNFNRSISNIVFMGMGEPLMNYNNMIAAIKKITQYRKFGISSRRITISTSGIPKMIMKLADEDFNFKLALSLHSANQSTREKIMPFTKKFPISDLLESLNYWYTKTNRIITLEYIVWDNINDKLSDINDLINFCKKIPSKVNLIQYNSIGDDYFKGANIEIINKYQSELLKHGINSTFRRSRGLDIDAACGQLANK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The presence of Radical SAM core domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MSRPNLVGMLPSALEDLAAEFGASRYRGRQLATWLYRKGVVSLDAMTDLPKDFRATLAERTVIALPELERATPSADGSRKLVFRLADGARISAVLMPDDRRVTLCLSTQVGCGYACAFCLTGTMGLARNLSAGEIVGQLLVANQLVGAHGRVTHLVFMGMGEPLANYGPLVTALRIFTDARLGMGYSPRRITVSTVGLAGAIDRLGREGLRVNLAVSLHATTDELRSRLMPVNRAWSLGALMAAVRRYPLAPRQRVFFEYVLLDGVNDSVEDARRLVALLRGIRAKVNLIPFNDWPGSGFRRPPLARILAFQAVLLDAGLTATVRWSKGEDIGAACGQLRER",
    "response#2": "MSRPNLVGMLPSALEDLAAEFGASRYRGRQLATWLYRKGVVSLDAMTDLPKDFRATLAERTVIALPELERATPSADGSRKLVFRLADGARISAVLMPDDRRVTLCLSTQVGCGYACAFCLTGTMGLARNLSAGEIVGQLLVANQLVGAHGRVTHLVFMGMGEPLANYGPLVTALRIFTDARLGMGYSPRRITVSTVGLAGAIDRLGREGLRVNLAVSLHATTDELRSRLMPVNRAWSLGALMAAVRRYPLAPRQRVFFEYVLLDGVNDSVEDARRLVALLRGIRAKVNLIPFNDWPGSGFRRPPLARILAFQAVLLDAGLTATVRWSKGEDIGAACGQLRER",
    "response#3": "STLLSIKTGGCEEDCGYCSQSAHHDTGLKAEKLMEVDAVLAAARTAKENGATRFCMGAAWRNPKDRHIEPIKEMIRGVKDMGLETCVTLGMLEEHQAKALAEAGLDYYNHNLDTSPEFYGQIISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLIAQLANMNPYPESVPINNLVAIEGTPLENAQALDPFEFVRTIAVARITMAKG"
  },
  {
    "reference": "MSQNDLNKEKALVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQRHDKEIEVAKKIAKEQNLKHHILDMSLLSQLTPNALTQHELSIEDNDDGIPNTFVPARNLLFLSFAGALAYQIHAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLSMDKDFVIHTPLMWLDKAQTWALSDKLGVLDYIRHNTLTCYNGIIGDGCGECPACHLRARGLKHYLEHKGEE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be able to bind Zn(2+), ATP ligand in a variety of conditions2. The designed protein should interact with enzymes and substrates within the 7-cyano-7-deazaguanine biosynthesis, Purine metabolism pathway to enhance or regulate its activity.3. A protein with ligase activity, forming carbon-nitrogen bonds, zinc ion binding, ATP binding functions is required to enable queuosine biosynthetic process. The designed protein sequence is ",
    "response#1": "MSVLLLA",
    "response#2": "MDTIVIATRNKKK",
    "response#3": "MTHLSNVVIATHNPGKVKELAGLLAPFGVK"
  },
  {
    "reference": "MKRMAIVLAAGQGKRMKSKLYKVLHPVCGKPMVGHVLNTVKQVNSERIVVVVGHGAEAVQSYLGNSAEYVLQEQQLGTGHAVKEVKSLLGDETGSTIVICGDTPLVTPETLEGLVQLHETNKAAATVLVAQMDNPKGYGRVIRGQDGRVLRIVEQKDCTPEEDAILEINTGTYCFDNAKLFAALERVNNNNVQQEYYLTDVIGILVEELETVLGYNTDDLAESIGVNDRLALSEAEQFMRKRIAKKHLLNGVTLIDPASTVIGADVIIGSDTIVYPGTILKGNTVIGEDCIIGPNSDIDSSTIANGAIVKHSVLDQAEVGSGTSVGPFAYLRPKTKLGDDVKIGDFVEIKNTTIDNGSKVSHLSYIGDAFVGKNVNVGCGAITVNYDGYNKYITKIEDDAFIGSNVNLVAPVTIGKGAFIVAGSTITHSVGENDMGIARQRQENKPGYADKIRAQAQAKKNNKG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).2. The UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA binding site should be located in a region of the protein that is accessible to the ligand.3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.4. The designed protein should have magnesium ion binding, glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity within cytoplasm to regulate cell wall organization, UDP-N-acetylglucosamine biosynthetic process, peptidoglycan biosynthetic process. The designed protein sequence is ",
    "response#1": "MSAVVLAAGKGTRMKSKVHKVLHPVCGKAVIDYIIDPLIEMGTEKRVVIVGHNAEAVENHLSERVTFARQEEQLGTAHAVMQAQDILSDQDGVTLVLNGDHPLFTKETLEELVRTHKESDAAATVLTAVLSDPTGYGRVIRNEDGTVDRVVEHKDASEEERTIREVNTGTFCFDNRLLWKALTRVNNDNAQGEYYLPDVLSILKSEGNLQCFGYQVKDYAETLGVNDRIALSQAEKAMRLRINEGHMRNGVTITNIASTKISADAVIGSDTVLLQGVTIDGKTVIGEECTIGPNSHITTSQIGDRTTVHSSVVTTSSVGNDTAVGPFAHIRPDSSIGNEAKIGNFVEIKKTQLGNQSKVSHLSYIGDAEVGERVNIGCGTITVNYDGKNKFVTKIESDAFVGCNSNLIAPVTIGEGAYVAAGSTINKEVPKDALAIARAKVPNYYIKKGGVTLRDPSRFDVRGEISVGRDVEIDVNVILEGRVSIGNNVKIGANVIISDSELGDDVVVQAHSIIESSQIGAGCRVGPYARLRPETVLDEKVHIGNFVEIKKTSVAQGSKINHLSYVGDAVVGANVNIGAGTITCNYDGANKHQTVIEDGAFIGSDTQLVAPVTVGKNATIGAGSTITRDAPQNALTISRVKGYTKN",
    "response#2": "VLVLAAGPGTRMRSDTPKVLHTIAGRSMLSHSLHAVAKVAPRHLVVVLGHDHQRIAPLVAGLAESMGRTIDVALQDRPLGTGHAALCGLSALPDDYAGIVVVTSGDTPLLDSDTLAGLIAAHTAAPAAVTVLTTVVPDPDGLGRIVRDGSGVVLRIVEEKDATEEQRRLREVNTGILAVPTVLLREWLARLDNNNAQGEYYLTDVVALAVADGVPVVAHHPARAWETEGINNKLQLAELERIWQRDTARRLLEEGVTLIDPDHTYIEADVEIGMDTIIYPGTILKGTTRIGADCMIGPSADLTDMHVEDGAEIRYSVLEGSTVSRTAKVGPYAYVRPGSSLGENSKVGCFVDVKKSTLGQGSKVSHLGYIGDSEVGQNVNIGCGAVTVNYDGQKKHQTVIEDGAFVGCNVNMVAPVTIGKGAYVAAGSTVTEDVPENSLAIARQRQKWIVEGWKRPRKR",
    "response#3": "VVIMAAGKGTRMKSKLPKVLHLLAGRALLQHVVDTAAQLSARQVVVITGHGAMEVEAAVSGNTGASAGFDINFVRQEPQLGTGHAVQQAVPLLRDDGMVVVLSGDVPLIQAATLRHLIAAAGTDRLGFLTVTLADPNGYGRILRDAEGAVQGIVEQKDATAEQLAITEVNTGILAAPARLLADWLGRLGNRNAQGEYYLTDVIAMAVADGLPVAAQRPETEVEVEGVNNRLQLAELERAHQLRQAEALLEQCGVTLLAPETVQIRGVVECGRDVVLDAQVTLEGQVRLGEGVTIGQGCVVRDSELAPGSRLEPYCVVEGVRSTGPCSVGPFARLRPGTQLAAGVKIGNFVEVKKARFAAGAKASHLSYVGDAQVGERANLGAGTITCNYDGANKHHTEIGADAFIGSNSALVAPVSIGAEATIGAGSVIGKDAPAAQLTLTRSRGPVRRETPPDG"
  },
  {
    "reference": "MISLRTHAARSLLALGALAAATSAAAQSGAPDRAVVGIAAVYTPGYQGGDDYRLMPFPVIDVTYGRFFASGREGVGYTVLDGSAVKVSAGATFVPGYRRRDAPVGVGRLDGGAGLRVAADMRLGEVLVSMSATKVVSGDVDGALVDASLAYPVRLSERLSLMPSVSATWADSQYNRAYFGISAAQSAASKLPVYRPGGGVKDVSMSLSANYRLNDKVSLGATASLSRLTGDARKSPIVVEATQPSAVLSVAYRF",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein localizes to the cell outer membrane.2. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "ELHRINYDLTFSLGFTNRIQTKKKNEVNFVAFYGTGLLIDWKEGNQYQPFRVYLATNVHVAAGLKNEADYLPYSQPDAELGWTVDFRLGKYTNVKDFVR",
    "response#2": "YQLPVVQSGTQSTASQGNRLLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGWVVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDGYDEMVKVLAGVWSQEAASIV",
    "response#3": "ELHRINYDLTFSLGFTNRIQTKKKNEVNFVAFYGTGLLIDWKEGNQYQPFRVYLATNVHVAAGLKNEADYLPYSQPDAELGWTVDFRLGKYTNVKDFVP"
  },
  {
    "reference": "MVWGTGYGNPSPLNPYGFASLHRGGLNPLILAPENITFDTLNTHNDHEETHGESPEVPKASIAPAGRQNVPVLQQNQENEDNHALGGSEDAKDEREIRFSAIKTLYNYYSEGPSTPIMPHLVNRLRGLDALAKIDATLTKVDMNAAYIFALRPTFPYSYGYKQRFSNRRLTTSALCYARTGLSSFLTVDKTYTSNSPLKGGSRGWPIFNVDVSSHVAEPHMRTLSPIGLEVFNLATSQFSKTLLTASSKVFTQSLYTADILSIFGEVFLPHVMQPVSNYTPILVRALLALIHILGPGSGNCSLSSSIFESSIPQFLTVSHSTNMSNRTRYCLHTRSAYKDMFRNGIPPQSTLPPTLAPEGSSARVLIPEALVTSPMFPWLLILVSSGPQFFLYSKDASINTVDIGSRGRITSPIPDVAKLDLHRLWNLFRFDGYRYIDVVIVGADRDYVWPYQNGVYVHGGKGPNGTGNYGNADVHDGIGTIFSSFNNNVNVQTSDLMLGLLTLWNHITTTYATEEEVTMAIKIAAAFALVYPVQPIVYSGCPKAFQRHTSYYQPSSENCYATDTAEVKSVWDTVELSVQVNNAMVLGMTLPFGQPTLSSAQWYNNIDKAEISMFKVGNLPLQNLDYLSLDMMEFYAPTTGQLYDIRSDNLILSAHRTVNLGIGYTALADFFAYLASVPAQSFYHDRMVTSPISKQTYSVYERFIERFIDDFVGWDRCDLFNLDTLLGAKHIAGVASSPIPWHCSLQRCPLPIIMHYTGLTFGQEHIT",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Displays ssRNA-binding activity .; RNA-dependent RNA polymerase which replicates the viral genome2. The protein must be able to RNA binding, hydrolase activity. The designed protein sequence is ",
    "response#1": "REVKAAFHSVQPDWNVTEANFAPFSRRMFSNFAQMEPDKEYLAHIILDSLKQAQADLDGNHYLNESLTEQARLDRNREESLNMVKDFERDFNNAAQRSAWSHKSTVPFPGVIPKVSGDTTSLSRLVELPVITGGSDATIRAWRSAYGSVSNGTVERCDEDVERERRAALCSLTVEELEESKALRMKYHRCKIDNGMMDKLDLAMQGVI",
    "response#2": "SSADNPISLFSLDVLRMIVDKLTERDNVIISSFIGEKIFPHIYPKVDVITQVFDLFDNWLYYKGNSGYRLMKTYEALVTGTILKRDKSNYHNSEDFLENTIAGLEQDEQEVARKFVHILDDSKPSPHHLTQIMGLFRLWGHPQVDAKKGVEKVRTIGTKKKYISEHASQIAGRKFKEIFFSEYFRKNRVYPACIVEEDCWLNEFIVQNLGINLKDPRYHLSDWDCVEPRETFSIPTTFNLSMIVSDTAISPTREEIFSCQEKGIPPMDPFIRRGVLKWMKDGLIDCDSLLKGINSCQTGLDKDNRIIGLYPKEREMNPTARMFALMSLKMRSYVVITENMLSENILPHVPGITMTYSMLDLAKEMIKSTRSQGKQGDFSRTFCINMDFEKWNLNMRKEATYYTFLEIGRLFGLPQLYNRTYDIFRNSLIYLADGSYTPKLDDNLDSLEKDQNLCYTDHIGGFEGLRQKGWTIFTVVLIAHVCDQLGIQYRLMGQGDNQVLMVTIHSKHARLNGIDSPGSKNEIAGKLQMLLRSLQDTFSEVGLPLKPLETWVSDTYFSYGKMPIYKGVPLCSSLKRISRIFYFSNEV",
    "response#3": "MEEQISEVKDIISKYLSNDDRLAKQKLAFLVQSEPKLLLIEGLKLLSLCIEIDSCEANGCDHNTKELSVENFLSENRVLCPGLPMVVPDGFKLNGNVLMILECFVRSSPANFEQKYREDLVKLNSLKEDLMTVGITMLPLIDGRTNFQTDRLPEWANERFRTLLFSLLAFSP"
  },
  {
    "reference": "MIIKPKVRGFICTTTHPVGCEANVRRQIAYTQAKGPIENGPKKVLVIGASTGYGLASRIAAAFGAGAATIGVFFEKPGTETKTGTAGWYNAAAFDKAAKEAGLYAKSVNGDAFSNECRQQVIELIKQDLGQVDLVVYSLASPVRKLPDTGEVVRSALKPIGEVYTTTAIDTNKDQIISASVEPATEEEIQNTITVMGGQDWELWMSALRDAGVLADGAKSVAYSYIGTDLTWPIYWHGTLGRAKEDLDRAAAGIRGDLAAHGGTAHVAVLKSVVTQASSAIPVMPLYISMSFKIMKEKGIHEGCMEQVDRMMRTRLYGSDMALDDHARIRMDDWELRDDVQQTCRDLWPSITSENLSQLTDYSGYKQEFLRLFGFGLDGVDYDADVNPDVQFDVVTL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must have a Proton donor active site that is highly conserved among related enzymes.2. The protein should have a high affinity for NAD(+), substrate.3. For general function, the protein need meet that Involved in the final reduction of the elongation cycle of fatty acid synthesis (FAS II)4. We require a protein that can specifically bind to the metabolites produced in the Lipid metabolism, fatty acid biosynthesis pathway.5. A protein with NAD binding, enoyl-[acyl-carrier-protein] reductase (NADH) activity functions is required to enable fatty acid biosynthetic process. The designed protein sequence is ",
    "response#1": "KPGSPGWYNTVAFQNEAKKAGIYAKSINGDAFSTEVKQKTIDLIKADLGQVDLVIYSLASPVRTNPVTGVTHRSVLKPIGGAFSNKTVDFHTGNVSTVTIEPANEEDVTNTVAVMGGEDWGMWMDAMLEAGVLAEGATTVAYSYIGPALTEAVYRKGTIGRAKDHLEASAATITDKLKSVKGKAYVSVNKALVTQASSAIPVIPLYISLLYKVMKAEGIHEGCIEQIQRLYADRLY",
    "response#2": "KPGSPGWYNTVAFQNEAKKAGIYAKSINGDAFSTEVKQKTIDLIKADLGQVDLVIYSLASPVRTNPVTGVTHRSVLKPIGGAFSNKTVDFHTGNVSTVTIEPANEEDVTNTVAVMGGEDWGMWMDAMLEAGVLAEGATTVAYSYIGPALTEAVYRKGTIGRAKDHLEASAATITDKLKSVKGKAYVSVNKALVTQASSAIPVIPLYISLLYKVMKAEGIHEGCIEQIQRLYADRLI",
    "response#3": "KPGSAGWYNSAAFDKAAKEAGLYSKSVNGDAFSDECRAKVVELIKADLGQVDMVVYSLASPVRKLPATGELIRSALKPIGEVYTATAVDTNKDELIEAHVEPANEEEIANTIKVMGGEDWELWLQALDQAGVLAEGVKTVAYSYIGTDITWPIYWHGTLGRAKEDLDRASSAIRQQLSSKNGTANVAVLKSVVTQASAAIPVMPLYIAMSFKLMKEQGIHEGCIEQIQRMFYTRLK"
  },
  {
    "reference": "MKYIGAHVSASGGVFNAPINATQIGAKAFALFTKNQRQWSAKALDNKTIDLWFKELEKSKIEPKHILPHDSYLINLGHPDSDAREKSIESFLDEVQRCEILALDRLNFHPGSHLRKISEEECLDNIAESMNRVIDKTSGVKLVIENTAGQGSNLGYKFEHLAYIIDKIEDKSRVGVCIDTCHMFTAGYDIRTREAYDKTWNEFEKIVGFKYLSGMHINDSKPELGSRVDRHDSLGCGKIGWDAFEFIMNDKRMNDIPLVLETIDESIWAEEIKTLYNFIK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate.2. The designed protein should be optimized for zinc ion binding activity in order to improve DNA repair.3. The Zn(2+) binding site should be stable and able to withstand changes in environmental conditions.4. The protein should be designed to have high stability and activity towards Endonucleolytic cleavage to 5'-phosphooligonucleotide end-   products..5. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "MPELPEVETVCRGLNQLTFGQTIRGGKVLLPRTLAYPVSIEEFLEQINNATFGQWQRRGKYLLVPLEEKKGWLGVHLRMTGQLLWVKQNEPLSRHTRLRLFCDRSKELRFVDIRTFGKVVD",
    "response#2": "GESVGLDSMQIFAKNERQWTAKPISPEEATAFRTEQQRTGIHPVVVHDSYLINLAAPADELREKSIAAFADELERCAQLDIPYLVTHPGAHTGIGEEAGLARVADAICRLLAEGVGGNTMILLETTAGQGTALGYRFEHLARLFELIPYHERLGICVDTCHIFAAGYDIRDPEGYQTTFAELDRLVGLTRVKCFHLNDSQKDLGSRVDRHAHIGQGCIGVEAFRMLVNDPRFADLPMIIETPKGEDMAEDRL",
    "response#3": "VERAEAAGATALQVFTRNQRQWNAPPLEEHDAELFTAAVRRWGGYPVSSHASYLINPAADTPEGAERSVALMADELQRAGRLGIEMVVVHPGAHKGQGEAAGAALAAARLDEALERCGSPAVMVLLENTAGQGTMLGASFAGLAAVIEASRMPERYGICLDTAHAFGAGYDLRDAALYDAAIRELDVCAGLDRLRLVHANDSLTGLGSRRDRHTHIGQGELGEAAFRLLMRDERLHHVPKVLETPKGKGPEEL"
  },
  {
    "reference": "MRLDVLLEGVEPLVVSGAANPDISAICDDSRDVKPGSLFVAIKGFQSDGNRFLADAASAGAVSVASEKPLAGFTEVFPNIAAVQVHDSRHAIGILARNFYGNPSSMLTMYGVTGTNGKTTVGYLVKTILETAGQKSGLIGTIGYEIGNQRLTTSHTTPSAAVLQSLLNRMVQQGVKEAVIEVTSHALALARISGCLFDTVVFTNLTRDHLDFHGDLETYFNTKLRLFSLDLLKLTTDTSTVRAVINLDDPYGDRVRDACGVSVWGYAIDSDADIRAENIVVTFEGTSFTAQTPVGSFLARSALVGRHNVYNILAAIGVGLSRGCSIKVVQQGIASLRCVPGRFEKIDEGQGYGVVVDYAHTGDALDKLLNTARSLTAGKIITVFGCGGDRDQGKRSVMGIIAARLSDLVIVTSDNPRSEDPDQIIRDVMVGIAASENTPPCEVIPDRKEAIVTAIQSAQPGDLVVIAGKGHEDYQIVGDRRLHFDDRRVAREAIVSAYGPLHD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein that can modulate the overall flux of metabolites through the peptidoglycan biosynthesis, Cell wall biogenesis pathway, enhancing or suppressing its activity as needed.2. The designed protein should have magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, tetrahydrofolylpolyglutamate synthase activity, ATP binding within cytoplasm to regulate cell wall organization, cell cycle, peptidoglycan biosynthetic process, cell division, regulation of cell shape.3. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.4. The presence of Mur ligase C-terminal, Mur ligase N-terminal catalytic, Mur ligase central domains is critical for the interaction of the designed protein with its intended targets.5. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.6. The designed protein should contain at least one Meso-diaminopimelate recognition motif motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "MNVTGTSGKTTTSYLLEAGLLHAGYSVGLIGTTGTRINREPVPTSLTTPEAPTLQELFARMVSEGVTHVVMEVSSHALSLGRVQGTRFDVGGFTNLSQDHLDFHPTMEDYFEAKALLFDGPHAADRSVICVDDEWGQRMAQRAAARPVIRVSTTSQGSDADITGRQLSTEVTGAQQVELTLASGEHAGTFEFRLPMPGTFNIANAALATGLALELGLAPGEIETALEGFGGVKGRVEMIPISDAFTLMIDYAHNAMALESLLTALRAYHPKRLVTLFGCGGNRSRDRRFEMGEVSGKMADFTIITSDNPRDEEPLAIIADIETGMKKTDGIYVMIADRKEAIRYAIMNAQEGDVIVLAGKGDEVQIRLGDTVVPFDDREVAREALLARLK",
    "response#2": "MKLRELLQGLTVEAVQGGENVVHDLQTDSREVRTGDAFVALAGTATPADQYIDKAIEAGAAVVLLETDVEQACYEYLGALIVPVKQLRQRLGKIAARFYGNRPSQELKMIGVTGTNGKTTSTYIMEKILSDQGYRTGLIGTIETIIGEKVIPATNTTPESFQLQQYFAQMVEAGCEYCVMEVSSHALELERVYGLEFAAGIFTNLTQDHLDFHKTMENYLNAKKRLFTMCDRAVVNRDDEYGRRLIEELPPHCVEFGVEGDYRAMNLTCQPSGINFSMHTPRGEIEVETRLVGKFNVYNALAAVGAALIEGVPAEAIQRGISQVKAVPGRVEMIPISDAFTLMIDYAHNAMALESLLTALRAYHPKRLVTLFGCGGNRSRDRRFEMGEVSGKMADFTIITSDNPRDEEPLAIIADIETGMKKTDGIYVMIADRKEAIRYAIMNAQEGDVIVLAGKGHETYQILKDRTIQFDDREEARKALARRME",
    "response#3": "MKLNELMQDIPYKLIEGDTQVTGVDLRAQDIEPGDLFAALPGTRSHGARFAADAVAAGAVAVLTDEAPAGVPQIVVSDTRIALAYAAGVFFGFPARQMRLIGVTGTNGKTSVSHYIAQLLNQADKRTGLIGTIQNRIGDQVLEAPNTTPDAIELQRLLAEMVGAGCERVVMEVSSHALALDRVDGCEFDVAVFTNLTQDHLDFHKTMEAYFAAKRRLFTMCKTSIVNIDDPYGRRLADEIPGECVTYGINADADVTADNIALSSRGTRFTLVTPRGQAEIELQMPGKFSVYNALAAIGACLIEGIPLEDIKTGLCKMKGVKGRVQRIERGQEFLAVVDYAHKPAALEAVIATLRGQSAGRLAVVVGAGGDRDTGKRPQMGEVAARSADLLVVTDDNPRSENPAEIRREVMAGALAVPVTERGEVREIADRAHAIADAVRWARSGDVVLVAGKGHETYQDIQGVKHPFSDVEVVREILDAS"
  },
  {
    "reference": "MIQVLLVTICLAVFPYQGSSIILESGNVNDYEIVYPKKVTVLPTGAMNSGNPCCDPVTCKPRRGEHCVSGPCCRNCKFLNAGTICNRARGDDMNDYCTGISSDCPRNPYKD",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have a specific function within the extracellular region. The designed protein sequence is ",
    "response#1": "GAPGRF",
    "response#2": "PGDGGPPGQPGPDGPPGAPGNAGEDGKPGDQGPRGPPGIPGAPGQPGRPGPPGEPX",
    "response#3": "GRAGAPGRPGPPGQPGPAGPPGENGKGGGQGPSGLPGPPGQPGQNGAPGEVGQPGDNGC"
  },
  {
    "reference": "MYDDIAYEPRGPDPGTTRFRLEISPVVTLPAPIIPLADVELIDAAAVALGQDLAALMEQAGAALAKEAQRLAPSGEILVACGPGNNGGDGYVAARLLAQAGRQVAVWPVVTPSSDLCRLNRDRLPAAARLVAAPPAKPPTLIIDAILGAGTRGDIRAPIPTALDALRRLGSPVLAADVPSGLGTPHLLPARFTVCFQAAKRELLGSGATAEFATVDIGLDPRAWLEVQPSVLRRFPHLPRNAHKGMNGELLVVGGGPFPGALELACRAAMATGCDLVRAWTCDGPPLPPTIVTHRHPGPLLERASTGELTPYAVRAGAVLVGNGLGRDPRAVDAALQAASLCLEMGIPVVLDADGIPACASLVEGADAGAPVLLTPHRGEARNLLGQPAAPDETQLHAWAATHRVLLAKAPVDLVSDGWRWQRNRRGNPRMAVGGTGDVLAGLSAGLMARGCQPYDAARIGILWVTEAGDELWLERGPCWDALDLIDRLPATLRRLFSEAGLAWPPVVD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..2. The designed protein must possess ATP binding, NADHX epimerase activity to support nicotinamide nucleotide metabolic process under certain conditions.3. The designed protein have YjeF N-terminal domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MQKADSFTINEIGLPSLVLMERAALKVVEAMEREQINLEKPLIFCGSGNNGGDGFAVARLLKEKGYDATAVFVGHESSMSADCRRQKEIAEKCGVPMVTTVCRQEYTTIVDAVFGVGLNREITGDYLESIVKMNALSGQKVAVDIPSGICSATGRVLGCAFQADLTVTFQCEKLGLILHPGHMYAGKTVVADIGIQCGLFEAADVRARWPVPGPNDDKYTQGVTGILAGSSTYPGAAVLCTGAAVAATSGMVRYAGSAKDEVLSHWPEVIASPTAAAAGRVQSWVVGPGLGTEDTGAAALWFALDTDLPVMVDADALTILAAHPDMVESRRAPTVLTPHAGEFARLAGGPPGDDRVAATRKLADALGVTVLLKGNVTVIAEPGGVVYLNPAGQSWAATAGSGDVLSGMIGALLAAGLPAPEAAAAAAFVHARAAALSAGDPGPGDAPTSASRIIPHIRTALAGM",
    "response#2": "MFPARKKDSNKGSYGKVLVIAGSDKMAGAAYFSAKAAYLSGCGLVQAFTPKANRDILLTMLPEALVTDYDSDHLDISLLREKMEWADVILIGPGLGTGEGAKVLVEEVLKREDKPLVMDADALNILSFEMELFRQSKAPIIVTPHLGEMSRLTGAKIPEIKERILHVAGEFAEKYGVLCVLKDSRTVIGTKKGLYLNISGCSGMASGGSGDVLAGFIAGLLAQNMSIEDGAVLGTFIHGRAGEKAALKKGEASMTARDILEEIGNVID",
    "response#3": "SHKYDRGVLGVVAGTPRYPGAAVLTVTAAVRAGVGMVRYVGPPGVTATVVAARPEVVAGYGRVQAWVVGPGVDPLDPPQAAHVRDALVAAHAARLPVVVDAGALDLLPDRVDPWVVLTPHAGELARLLAERGEIVERGDVEAHPLTWARRAHALTGATVLLKGATTVVVGAGGAAYSQQDAPAWLATAGAGDD"
  },
  {
    "reference": "MRAWEEFLLLQEKEIGVDTVNKWLRSLKVLCFDACNLYLEAKDSFQVTWFEEHIRHKVKASLINNNGKPIRVRVTSLDKSTPFKETQIQQEKTAYFTMKYGDIDPNMSFANFLVTPENDLPVRILQEFAKVSEQGKGFPFNPIYLFGPESSGKTHLMQAAVGGLREAGVKTLYVTSELFTEHLVSAIRSGEMQRFRAFYRNVEALFIEDIEVLSGKGATQEEFFHTFNSLHTEGKLIVISSTFAPGDLKAMEERLISRFEWGISIPVSPLIREGLKSFLERRTEKLNIRIEETALDFLIQALSSHVKSLLHALTTLAKRVAYKKLSHQMLYQGDIEALLHDVLQAAESIRLTPSGIVRATAQYYGVSPESVLGRSQSREYVLPRQVAMFLCRQKLSLSYVKIGEVFSRDHSTVISSIRAISQKLEEDDRECDVSRAIQELTKRLSSAYQSLDFIED",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have ATP binding, DNA replication origin binding within cytoplasm, plasma membrane to regulate DNA replication, regulation of DNA replication, DNA replication initiation.2. The protein should have a high affinity for ATP.3. The protein must exhibit the following characteristics: Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'- TTATC[CA]A[CA]A-3'; DnaA binds to ATP and to acidic phospholipids .; Plays an important role in the initiation and regulation of chromosomal replication. The designed protein sequence is ",
    "response#1": "LDSRFTFDTFVEGSSNRVALAAAKTIAEAGQGAVRFNPLFIHSTVGLGKTHLLQAVANAAVQNPRSLRVVYLTAEYFMWRFATAIRDNDALTLKDSLRNIDLLIIDDMQFLQGKMIQHEFCHLLNMLLDSAKQVVVAADRAPWELESLDPRVRSRLQGGVAL",
    "response#2": "AYNPLLIYGGTGLGKTHLLNSIGNYNVAKGKSVIYVTSEQFLNDYLYHIRNNTMDRFRDKYRACDYLLIDDVQFFGGKPQIQEEFFHTFNELHNKNKQIVLTSDKTPKQIAGLEERLKSRFEWGMVSDIR",
    "response#3": "LKEKYTFANFVQGDENRWAVSASIAVADSPGTTYNPLFIWGGPGLGKTHLLNAIGNQVLRDNPNARVLYITAENFINEFVSHIRLDSMEELKEKFRNLDLLLIDDIQSLAKKTLGGTQEEFFNTFNALHTNDKQIVLTSDRNPNQLNDLEERLVTRFSWGLPG"
  },
  {
    "reference": "MLKVNEYFDGQVKSVGFESAGDHASVGVMAPGEYTFGTAAPERMTVVKGALIVKLPGHVDWETYNAGDDFEVPGDSSFNVKVLETTAYLCDYL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must have high specificity towards guanosine phosphorylase activity, thymidine phosphorylase activity, pyrimidine-nucleoside phosphorylase activity, uridine phosphorylase activity, purine-nucleoside phosphorylase activity.2. The protein must exhibit the following characteristics: Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates; Also catalyzes the reverse reactions..3. The protein should have a low Km value for phosphate + xanthosine = alpha-D-ribose 1-phosphate +   xanthine. The designed protein sequence is ",
    "response#1": "GFVD",
    "response#2": "METNQDEIL",
    "response#3": "MEIILANPRGFCAGVDRAISIVENALELHGAPIYVRHEVVHNRFVVDGLRDRGAIFVDEIAEVPDDA"
  },
  {
    "reference": "MLTVLTAEQMRAMDQWAIEEMGIPGVVLMENAGTAIVRRLQEIFPDLPAKKIIIFCGKGNNGGDGFVIARHLSQLGANAAVLLAGTLVELKGDAKTNALSAENLEIPIQELNPENINKFDHKLRHADIFVDALFGTGLSKPVTGFLETVINKINQHEKYTVSVDINSGIDSDSGKLIGPHVLSDITFALASMKQSHLLHPSAGAMKKVEVLDIGIPERDDKNVQIHLLEEEDIKSIFQPRQPDAHKGSYGHVLVLAGSRGKTGAAGLTALAALRSGCGLCTLALPETCQKAFELHPMEVMTVPLPETASGSLSIKAKEPILKLLEGKSIAAMGPGLTTDPETVELIGEILPLIQCPLVLDADALNALEKNIDWLDNVKSAVLTPHPKEMSRLTGMSTQEIQEDRIGVTTRFAQKHSLVLLLKGAPSLIGTPDGNVYINPTGNPGMATGGSGDVLTGIIAGLAAQNISLKNASLAGAYIHGYTGDLFAESESQTNLIAGDLLRNLPDALKRVLP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit metal ion binding, NADHX epimerase activity.2. The designed protein should have a binding site that is specific to the target AMP ligand.3. The designed protein must have at least one YjeF N-terminal domain.4. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. The designed protein sequence is ",
    "response#1": "LLVVAGSLGMTGAAVLASRAASQCGTGLVYLATARSLVLPLAAHLTEQVMVPLAENEAGGISPLSLPELLPVLQKSTACVAGCGLKNTEDTRSIIRSIITNAECPVVLDADGINSVVGDINIIRCAKNGLILTPHYGEFSRLTGKTVEYIAQNRVALAKAFAQEYKVTLVLKGTTTVVALPDGSVCLNKGSNSGLAKAGSGDLLAGIIGGLLAQGLGINEAAICGVYLHSQAARLTAKRLCKTSMQPSDILL",
    "response#2": "PLSKKGDNGRVLIIGGSRLYSGAPALSGMAALRAGIDIAVITTPKSAAWIIKTFSPALIVWPLKSDEYLGLDDIEYIMSVKERFDCIILGPGLGLNEETMEAVREIVRTVDKPMVIDADGLKALKDNLDLIRNKQVVITPHAGEFAIVTGINVSNMSWNERMEIVKEIARKYNITILLKGKLDIISDGKKVRINCTGNASMTVGGTGDTLTGIIGAMLSRGINTFDAACAAAFINGLAGELASEKYTYITPLDLVQEIK",
    "response#3": "VLVIGGDLGTGGAALLSAEAALRTGAGMVTLATRPEHVTAALVRRPEIMCSGVESTYGLAALARRADVVIIGPGLGQAPWGRSLLSLAPQCRVPQVWDADALNLLAEGAVEPPADCLLTPHPGEAARLLGISVAEVQADRPAAVQQLAIRYGCSVLLKGAGTLIADPTGRLMLCDRGHPAMASAGLGDVLAGVAGALLAQGFVPFDAACLAVWLHAVAGERVGAA"
  },
  {
    "reference": "MATMIPPVKLKWLEHLNSSWITEDSESIATREGVAVLYSKLVSNKEVVPLPQQVLCLKGPQLPDFERESLSSDEQDHYLDALLSSQLALAKMVCSDSPFAGALRKRLLVLQRVFYALSNKYHDKGKVKQQQHSPESSSGSADVHSVSERPRSSTDALIEMGVRTGLSLLFALLRQSWMMPVSGPGLSLCNDVIHTAIEVVSSLPPLSLANESKIPPMGLDCLSQVTTFLKGVTIPNSGADTLGRRLASELLLGLAAQRGSLRYLLEWIEMALGASAVVHTMEKGKLLSSQEGMISFDCFMTILMQMRRSLGSSADRSQWREPTRTSDGLCSLYEAALCLFEEVCRMASDYSRTCASPDSIQTGDAPIVSETCEVYVWGSNSSHQLVEGTQEKILQPKLAPSFSDAQTIEAGQYCTFVISTDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEPHRSIKKVSSSKGSDGHTLAFTTEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISNVGEVSCGSSHTIALSKDGRTVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCGACLGCGSSEATALRPKLIEELAATRIVDVSIGDSHCLALSHDNEVYAWGNNSMGQCGQGNSTGPITKPKKVSGLDGIAIQQISAGTSHSLAWTALPRDRQVVAWHRPYCVDLEESTFSHLRSFLERY",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should contain one or more B30.2/SPRY domains that are essential for its function.2. A protein that can perform guanyl-nucleotide exchange factor activity in Golgi apparatus, membrane, cytoplasm, cytosol to support cerebellar Purkinje cell differentiation, autophagy, neuron projection development.3. A protein should be able to manipulate the activity of enzymes in the Protein modification pathway, either by activating or inhibiting their function.4. The design of the protein must focus on the precise arrangement of amino acids in the Glycyl thioester intermediate to enable effective catalysis of S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +   [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-   cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine..5. For general function, the protein need meet that Binds phosphatidylinositol 4,5-bisphosphate, which is required for GEF activity The designed protein sequence is ",
    "response#1": "VDKKMVEKCWKLMDKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTILSRYEGKMEALGENEYFRVFMENLMKKTKQTISLFKEGKERMYEENSQPRRNLTKLSLIFSHMLAELKGIFPSGLFRNWNSLAVTHPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRNQNPDLTGKSNCEKLKQGIAVRFHGEEGMGQGVVREWFDILSSEIINPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGEILGLALYHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGASILVTQENKAEYVQLVTELRMTRAIQPQINGFLQGFRNWNSLAVTHPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRNQNPDLTGTHPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRNQNPDSKIQLSVTIEEASLRNNGFLKPNPYVELLIDSKSKRKTDLVKNSYLPKWNEEFTVLITPNSTLHFKVLDHSSFRKDAMLGERIIHLAHILQHYNGRCLFSNRSAPLLLPKGTDVDYCLKMLKFTRRLLLNTENRFVYSSGDVLMYLLNCPNFTIKLAVMRILAILGERFVIAREKIVAHNIFGDHNLRKKTLKLALSLSSSVMDEDGEHFSLVDLYFDKKKVPQKWRKLRFTHYTSNDFKKSSQQKNNINETQTSIKKVTMTTQELCEHSLQQIFDKGMALLPAESWFDFSIKASVAKAFSDDSGENIDLRNIIIETKLNAIAFVNTIFSPPQVSSKLFELDPYAFNSLTDLISLSETKIPKELRTDALFTLECISLKHVWCSDIIRNLGGNISHGLLFQILRYI",
    "response#2": "MVMADQHRSVLELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKRGADPNYQDISGCTPLHLAARNGQKKCMSKLLEYNADVNAQEREGLTAIHWLAVNGRTELLHDLVQHVTNVDLKDPMGQTALHVACQNGHKTTVLCLLDSGADINLKSNCEKLKQGIAVRFHGEEGMGQGVVREWFDILSSEIINPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGEILGLALYHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGASILVTQENKAEYVQLVTELRMTRAIQPQINGFLQGFSATDLKKRLMIKFDGEDGLDYGGLSREFFFLLSHEMFNPFYCLFEYSAHDNYTLQINPHSGINPEHLNYFKFIGRVVGLAIFHRRFLDAFFIGALPDVDYCLKMLKFTRRLLLNTENRFVYSSGDVLMYLLNCPNFTIKLAVMRILAILGERFVIAREKIVAHNIFGDHNLRKKTLKLALSLSSSVMDEDGEHFSLVDLYFDKKKVPQKWRKLRFTHYTSNDFKKSSQQKNNINETQTSIKKVTMTTQELCEHSLQQIFDKGMALLPAESWFDFSIKASVAKAFSDDSGENIDLRNIIIETKLNAIAFVNTIFSPPQVSSKLFELDPYAFNSLTDLISLSETKIPKELRTDALFTLECISLKHVWCSDIIRNLGGNISHGLLFQILRYISATDLKKRLMIKFDGEDGLDYGGLSREFFFLLSHEMFNPFYCLFEYSAHDNYTLQINPHSGINPEHLNYFKFIGRVVGLAIFHRRFLDAFFIGAL",
    "response#3": "ENGLILFVVGCCMRQTVVTTWVLHLALSDLLATASLPFFTYFLAVGHSWELGTAFCKLHSSIFFLNMFASGFLLSAISLDRCLQVLRPVWAQNHRTVAVAHRVCLALWALAVLNTVPYFIFRDTIPRVDGRIMCYYNVLLLNPGPDRDATCNSRQTALAVSKFLLAFAVPLAIIASSHAAVSVHLRHRGRRRPGRFVRLVTAVVAAFALCWGPYHVFSVLEARAHADPALRPLVWRGLPFVTSLIK"
  },
  {
    "reference": "MSAAKLNVKTSAKPNSRIAVEVEVPANRCKNSYDEALSKLSRSISIPGFRKGKVPKTVVIQQLGVKRIQASALESLLQKVWTETLDQEGIEPLCEPELEDGFETILENFNPEKTLILKLETDITPIPTLKKSSGLTAEVENLIFDPKKVDELIEQSRAQLATKVPVSDRAAQKGDIALVSFKGSFSDDGSEIEGGSADSIEIELEQGRMIPGFIEGVIGMNINDEKTLKCEFPKDYHQEEAKGRKAEFNVSLEDLKIKELPELNDEFAKQASDKENMSDLRADLEKRLKEDNDRKQAKTRQDSLLDVLVKELEVDLPKSLIDQEVRTIVEQTAQNFAQQGIDVKSMFTPELVKSLMESSKGEAEKKLRQKFALQALAKSEKIEVSDKEINSKLEQVEADIKLSNEKNIDAERLKEAITDDLLQEKLFAWLEENNTVVEKTPEKARDQIKEKSSKKKTTKTNKEKKSSKTPKS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Functions as a peptidyl-prolyl cis-trans isomerase..2. The protein should be able to modulate cell division, cell cycle in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "LDEAKEDIKKRMDRNQQEHANQMNTMLLTQFANEDCQFSHIPKSLLDREVNLLYQQFANQMQQIKMSVEDALKIQNQTKEQLYQRLTEQATRAIKLVLVLEEIGDLEKISVSEAEINQEIEERIKEISNNQELPKEQYDAIKNYFVTQKELIESMLTNKKVVDLIV",
    "response#2": "LDEAKEDIKKRMDRNQQEHANQMNTMLLTQFANEDCQFSHIPKSLLDREVNLLYQQFANQMQQIKMSVEDALKIQNQTKEQLYQRLTEQATRAIKLVLVLEEIGDLEKISVSEAEINQEIEERIKEISNNQELPKEQYDAIKNYFVTQKELIESMLTNKKVVDLIS",
    "response#3": "LDEAKEDIKKRMDRNQQEHANQMNTMLLTQFANEDCQFSHIPKSLLDREVNLLYQQFANQMQQIKMSVEDALKIQNQTKEQLYQRLTEQATRAIKLVLVLEEIGDLEKISVSEAEINQEIEERIKEISNNQELPKEQYDAIKNYFVTQKELIESMLTNKKVVDLIV"
  },
  {
    "reference": "MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQPREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQYEELIAAYEKQRPAYWQD",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be expressed specifically in the 3-isopropylmalate dehydratase complex2. We need a protein that can perform (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate with high efficiency. The designed protein sequence is ",
    "response#1": "MDRENVDTDAIIPKQFLKSIKKTGFGPNLFDEWRYLDKGEPGVPESQRKPNPDFVLNQPRYQGASILLARKNFGCGSSREHAPWALDQYGFRAIIAPSFADIFFNNCFKNGLLPIVLPEATVDQLFNEAAAFPGYQLTVDE",
    "response#2": "MKSFTVLDGLAAPMDRANVDTDMIIPKQFLKSIKRTGFGKNLFDELRYLDEGKPDQSCEGRPLNTEFPLNFPRYQDASVLLARENFGCGSSREHAPWALDDYGFRSVIAPSFADIFFNNCFKNGLLPIMLDEA",
    "response#3": "VDLVYAHDGTMPLIIEAFNRIFNEVRTRAYIFFDHVYPAPTVKVANLHREIREFAKIHGIPVVEGKGISHQLVVELGLAEEAKIIVGGDSHTPTLGALGVFAVGMGATDVAIALGLGKIWLKVPESIAVLLDGSPREYIMASDVMIHLITSLKDREMSYNAIEFFNVPFSFDERLTLTNFSVEANAKTAIIG"
  },
  {
    "reference": "MGRGAIVLAAGLGTRMKSKTHKVLHQVCEKPMIVHILDELEKLDLDQLIVVVGQQREAVEIVLQGRAQIAYQAEQLGTGHAVSAAASLLRPDVDTTVVLYGDAPLIQAETIERLLVEREQRQASAVVLTATVTDPTGLGRVTLDADGAVVRIVEEKDATPAEREICFINTGIYAYQTTDLLRSLAQLRPDNAQGEYYLTDTLAILRQENKLVSALEVEDATEIASVNDRVQLAQVEQLMRQRINRSWMKQGVTLVDPSATYIGLDVTIGQDTVLLPGTILGGRTQIGSACVIGPNTRLIDVIVGDEAKVEYAVALQSDIGPRASVGPFAYLRPGSVIGERVKIGDFVEIKNSTIGDDSKVSHLSYVGDTSVGQRVNVGCGVVTVNYDGRGKYRTIIGDDAFVGSNVNLIAPVQVGDGAYVVAGSTITDEVPADGFAIARSRQVTKPNYVKSWQGLRFGKENSRGKTDECPETET",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be involved in UDP-N-acetylglucosamine biosynthetic process, peptidoglycan biosynthetic process by localizing to cytoplasm and exhibiting glucosamine-1-phosphate N-acetyltransferase activity.2. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc); The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.3. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.4. The designed protein must have at least one MobA-like NTP transferase domain.5. Mg(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "VVIMAAGKGTRMKSALPKVLHKLAGRSLLQHVLSTVSALGPQQRTVVITGHGAEAVEAAVTAPGLQFVRQQPQLGTGHAIQQVSPALDTALDTTLILNGDVPLIRAETAQALVQACGEDQLALLTVELADPNGYGRIVRENGQVVAIVEQKDASPEQLAIREVNTGIMAVPSGRLKRWLAQLSNDNAQGEYYLTDIVAMAVQEGVPVVTAQPAAAWETLGVNSKVQLAELERIHQRNVAAALLERLLEQGITLLDPSRFDLRGEIVSHGQDVEIDVNVLIEGQVKLGERVKIGPNVIIKDSHIGDDVEIFANSLIDRANIGAGSHIGPFARIRPESTLGEYVHIGNFVEIKKTTVGNSTKINHLSYIGDSIIGSNVNVGAGTITCNYDGANKHLTIIEDGAFIGSDTQLVAPVKIGRNATIGAGSTITKNAPDGQLTLSRSKQRNIADWKRPVKRKK",
    "response#2": "MENGVTLPAPETVHFAFDTAIGRDTVIEQNVVFGPGVTIETGAHIRAFSHLEGCHVSRGAVVGPYARLRPGAELAEDTRIGNFVEVKNATIDEGAKVNHLSYIGDAHVGSGSNIGAGTITCNYDGVGKHHTEIGENVFIGSNTMLVAPVTVGNGALTGSGSVITKDVPADALAIARSQLEFPSSPDSAKVLHPIAGQPILNHVLTAANSLNADHTVVVVGHQREAVLDYLLKQHPTVITAVQENQNGTGDATSVGLAALATAGVTLAPADTVVVLAGDIPLIRPETLQPLAKLSHDAVVTAFSPDDPVRLGPMRIEGDRLLSIEEWKASDEERAITLCNSGVIAAESDVLFNLIDAIGNDNASGEYYLTDIVEIARARGLTCKVVEGAEAETMGINTREQLAEAERAFQARCRARA",
    "response#3": "MAALILAAGKGTRMPSPRPKVLQTLVGESMLGLVYAALAALPGVERVLTLVGYEADLVRAEAERCARRGPRPDEAANCIEQVEQRGTGHALMTALPELENGCGRVLVVNGDTPLLPSAALRRFVTEAAGADLAFATVVADDPSGYGRIVRDSDDGAVRGIVEHRDATAEQLAIREINSGVYAFDAAVLREAVAGLTADNAQGEEYITDVLAILRGDGHRVGAAVAGDWVETEGVNDRVQLAVLRAELNRRLLDRWMRAGVRLADPARFDLRGRLGCGQDVDIDVGCVFEGEVTLGDGVRVGPYCVIRDARIDAGTQLAAFTHIDGAQVGAGAQIGPFARLRPGAQLGDEVHIGNFVEVKNSTLARGAKANHLAYLGDATVGERVNYGAGSITANYDGANKHRTVIGDDVHVGSNCVLVAPVTLGAGATIGGGSTIAKDAPAGQLTVARAKQRNVEGWVARRRAGSRAAAAAEAAAQARAADETTARAAASEDTIEP"
  },
  {
    "reference": "MTKFVFVTGGVVSSIGKGIVAASLGRLLKSREYSVSILKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTDTSMSRLNSVTTGSIYQAVLNKERRGDYQGGTVQVIPHITNEIKERVLRVAQNTNPDVVITEIGGTVGDIESLPFLEAIRQFRKDVGRQNVLYMHVTLIPWIPAAGEMKTKPTQHSVKELRSIGIQPDILVCRSDRPLPPGLKEKISGFCDVPASCVVACPDAGSIYEVPLVLEREGLANQALDLLQLEQRNPDLTQWGILVERLYHPIHKIEVAIVGKYVQLGDAYLSVVEALRHAAIALDSALNIRWVNSEEVEVKGADVLLQGVDGIIVPGGFGPRGVDGKIDAISYARQQKIPFLGLCLGMQCSVIEWARNVSHLHEANSSEFNPSTQNPVINLLPEQQDVVDLGGTMRLGLYPCRLVPNTLAFNLYREEVVYERHRHRYEFNNAYRNVFVESGYTISGTSPDGRLVEIIELPNHPFFIATQFHPEFQSRPNHPHPLFKGFVQATLSHNPSTSLNSDESRSLSSDASEADIEMQSAGLR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A key design requirement for the protein is the optimization of the Nucleophile to achieve high catalytic efficiency for H2O + L-glutamine = L-glutamate + NH4(+).2. The protein must be able to metal ion binding. The designed protein sequence is ",
    "response#1": "VRIGLVGKYVELPDAYKSIIEGFVHAGSKNECKVKVEYIHSEGIYPDNAHEKLGHLDGVLVAPGFGSRGIEGKIDTIRYVRENNVPFFGICLGMQCAVIEYGRNVIGLEGAHTTEIDENCAHPVINMMEEQKKITEKGGTMRLGSYPCDLKKGSKAFAIYGRTHITERHRHRYEFNSAYLQQYEDAGMIASGINPESKLVEIIELKNHPFFIGGQFHPELKSTVANPHPLFVKFIKAAG",
    "response#2": "LPDSYISITEALRHAAAYLGVGVKLRFIEAEELEKDPSRLEELEGCDGVMVLPGFGQRGAEGKIMAAGYARERGIPYLGVCFGMQLAVVEFARNVAGLEGANSTEVDPRTPHPVIDLLPEQAGIDRMGGTMRLGAQASILEPGTLAFKLYGQPVVFERHRHRYEVNPAYWELLQRKGLVFSGYSPDKRRVEFIELPNHPFYLGCQCHPEFKSRPGRPSPLYLGFIR",
    "response#3": "VTVAVVGKYIDLNDSYKSIDEALFHGGVANSVRVQLRKIDSEKLEKAEDLDEVFQGVDAILVPGGFGSRGINGMVRTANYARTHAIPYMGICLGMQVMVIEYARNVLGFEDADSTEFGPESGHPVVSLLEEQVNIKNYGGTMRLGANDSRLMAGSVIRKLYGSETIRERHRHRYEFSNQYREQLHNAGLLVSGVTPDDSLVESVEWNGHPWGIGVQFHPEFQSKPVATHPLFAGLILKAM"
  },
  {
    "reference": "MNKVNFRILGLQKYQDIYYIMQKFITCLKKNNINEIWLLEHYPVFTQGNSDNFNKKYIFNIPVVKTDRGGHMTFHGPGQKIIYFLLNIKNLNIKISKLIFYLENIIISTLSYFKINSYSIKNSPGVYVDKKKICSIGLRIKDGYSLHGLALNVDMDLYPFSHIHPCGDKNIKMTQIRDLISNINLEKLNTQIINNCKKFLMMNNFEINFLNSIKIF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate- dependent enzymes2. The protein should be able to modulate protein lipoylation in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "PSNKQAVWMLEHFDCYTLGRGASEDNLLFDVEKPPIDFYRIDRGGDVTHHLPGQLVVYLVLDLRRYKTDLDWYLRQLENVLLDVLDGLGLNGYRINGMTGVWCNGKKVASIGISCRRWITQHGIALNVDCDLLGFNQIVPCGLKDYQTGRLNSWLPKLQMKEVRFLMKKSLNKRFGLLA",
    "response#2": "YLTAWQAMKNFTAQRTCETPDEIWLLEHPPVYTQGIAGKPEHLLFPNQIPVIKTDRGGQITYHGPGQIILYLLLDLHRWRLNIRQLVRKMEGAVIDLLSEYDVVAQGDENAPGVYVDGAKIASLGLKIRRGACYHGIAFNADMDLTPFTAINPCGYRGLRVTQAKDLGIADCKEMLL",
    "response#3": "MILGDVCTRACGFCNVTTGRPQGLDLDEPVRVAKAIAAMGLSHAVITSVNRDDLPDGGSGIFAQTVREVRRRLPECDVEVLIPDFKGVRADLERVMAEGPAVLNHNTETVLRLQRDVRTSARYGRSLALLARAKWYRPEGVVKSGLIVGMGETRAEVLGALL"
  },
  {
    "reference": "MDSLSPFRAKILVLGDVMLDKYYHGDVSRISPEAPVPVVKVCEERLSAGGAGNVAVNLVNLGCSVYIAGALGEDQSGESLRKILLEKGVNISGLVKRPYPTITKIRVLGGRQQVVRVDFEEIVPLSRQEEEKVLEWFLKELPDSDCVVLSDYAKGFCTVGLCRFIIEESKRRSVPVVVDPKGPDWGKYSEAFIVTPNVKELGESCGRAVNNVDDEIVKAGLEVLRRYKLKNLLVTRSEKGMTLFVGEEVHHIPTKAREVFDVSGAGDTVVATLSACLATGMELVESAKMANIAAGVVVGKVGTAPITLSELEEALKNSKFSMSVESKIKGLDELLEIVRRWKRDGLKVVFTNGCFDLLHVGHIYSLEEAKKRGDKLIVGLNSDASVRRLKGEGRPIVSQRDRARMLAALEFVDCVIIFDEDTPERILSLIRPDVLVKGGDYRVEDVVGKEYAREVHIVPLFKGYSTTSLIRRIMNGS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a low Km value for ATP + D-glycero-beta-D-manno-heptose 7-phosphate = ADP + D-   glycero-beta-D-manno-heptose 1,7-bisphosphate + H(+).2. A protein with phosphotransferase activity, alcohol group as acceptor, ATP binding, heptose-1-phosphate adenylyltransferase activity, heptose 7-phosphate kinase activity is needed to enhance lipopolysaccharide core region biosynthetic process, ADP-L-glycero-beta-D-manno-heptose biosynthetic process in a specific context.3. The presence of Carbohydrate kinase PfkB, Cytidyltransferase-like domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MVVGDVMLDRYWYGNTHRISPEAPVPVVHVQQQDERPGGAANVALNIRTLGAHTLLLGTTGLDEEANSLSKKLHDAKIECHFQIIKAPTTTKLRLISQQQQLIRIDFEQSSSTEHLHELLTYFDNLIQKTKLLVLSDYNKGVLRYAPQFIQKARKKNIPVIVDPKGHDFSIYRDATLLTPNKKEFEIIVGPCQTQEILVRKAKELIEQLNLTALLITEGAQGMTLVQKNLPHVHFPAHQHEVFDVTGAGDTVIATLAVAMALGYELEPAVYLSNVAAGIAVTKLGNATITFSEVIGDAMLDTYFEGTAARLCSEGPVPIVRKTSERHLPGGAANTAANLRALDAQVMLLGFVGRDNAGNLLRAALRERGIDDTWLIEDERASTLHKLRILADGQFVVRFDDEDAHHYAPPGQQLLLARLEEMFPRCDVVIVSDYCYGVISDALIDRLQFLHAAHPNVLLAVDSKNLRRFKHAGATVITPNLLEAQLLVTQNDDMQGLLPAVPEQASTPFPEAERIGQRLLSMIDAEHAVITMAADGVMLVDRQGRARHIPAHPVHHANDIGAGDSFVAAMALSLAAGAQLDEAARIGIDAAEIAVTKRWDVERQRGRTIVFTNGVFDILHAGHVHLLRQAKQLGDVLVVGVNSDQSAQRLKGKHRPINSERDRLALVAALDPVDYVALFDEDTPATLIRTLRPHIHVKGGDYADEQLPEAGAVHEVGGRIVIIPLVGSMSTSSVIDRIQHSEK",
    "response#2": "MVVGDVMLDRYWYGNTHRISPEAPVPVVHVQQQDERPGGAANVALNIRTLGAHTLLLGTTGLDEEANSLSKKLHDAKIECHFQIIKAPTTTKLRLISQQQQLIRIDFEQSSSTEHLHELLTYFDNLIQKTKLLVLSDYNKGVLRYAPQFIQKARKKNIPVIVDPKGHDFSIYRDATLLTPNKKEFEIIVGPCQTQEILVRKAKELIEQLNLTALLITEGAQGMTLVQKNLPHVHFPAHQHEVFDVTGAGDTVIATLAVAMALGYELEPAVYLSNVAAGIAVTKLGNATITFSEVIGDAMLDTYFEGTAARLCSEGPVPIVRKTSERHLPGGAANTAANLRALDAQVMLLGFVGRDNAGNLLRAALRERGIDDTWLIEDERASTLHKLRILADGQFVVRFDDEDAHHYAPPGQQLLLARLEEMFPRCDVVIVSDYCYGVISDALIDRLQFLHAAHPNVLLAVDSKNLRRFKHAGATVITPNLLEAQLLVTQNDDMQGLLPAVPEQASTPFPEAERIGQRLLSMIDAEHAVITMAADGVMLVDRQGRARHIPAHPVHHANDIGAGDSFVAAMALSLAAGAQLDEAARIGIDAAEIAVTKRWDVERQRGRTIVFTNGVFDILHAGHVHLLRQAKQLGDVLVVGVNSDQSAQRLKGKHRPINSERDRLALVAALDPVDYVALFDEDTPATLIRTLRPHIHVKGGDYADEQLPEAGAVHEVGGRIVIIPLVGSMSTSSVIDRIRG",
    "response#3": "MVVGDVMLDRYWYGNTHRISPEAPVPVVHVQQQDERPGGAANVALNIRTLGAHTLLLGTTGLDEEANSLSKKLHDAKIECHFQIIKAPTTTKLRLISQQQQLIRIDFEQSSSTEHLHELLTYFDNLIQKTKLLVLSDYNKGVLRYAPQFIQKARKKNIPVIVDPKGHDFSIYRDATLLTPNKKEFEIIVGPCQTQEILVRKAKELIEQLNLTALLITEGAQGMTLVQKNLPHVHFPAHQHEVFDVTGAGDTVIATLAVAMALGYELEPAVYLSNVAAGIAVTKLGNATITFSEPDFSGLRVLVAGDLMLDRYWHGATERISPEAPVPVVHVREEEGRPGGAGNVAHNLAVLGGGVTLLGLTGEDEAADRLEEKLSASGVNCAFLRDGRFPTITKLRIVSRHQQLIRLDFEDGFPGHEPGALLERFRERLPGHQVVVLSDYGKGTLGDPRAWIGVAREAGVPVLVDPKGRDFGRYAGATLLTPNLAEFEAVAGPCADEAELEARALELRERLGLDYLLVTRGEQGMTLVGGEGCHHLPTRAREVFDVTGAGDTVIATLAAALAAGESMEAATALANLAAGVVVGKLGTAGVTRAELERDVERQRGRTIVFTNGVFDILHAGHVHLLRQAKQLGDVLVVGVNSDQSAQRLKGKHRPINSERDRLALVAALDPVDYVALFDEDTPATLIRTLRPHIHVKGGDYADEQLPEAGAVHEVGGRIVIIPLVGSMSTSSVIDRIQQ"
  },
  {
    "reference": "MDKFRMLFQHFQSSSESVMNGICLLLAAVTVKLYSSFDFNCPCLVHYNALYGLGLLLTPPLALFLCGLLANRQSVVMVEEWRRPAGHRRKDPGIIRYMCSSVLQRALAAPLVWILLALLDGKCFVCAFSSSVDPEKFLDFANMTPSQVQLFLAKVPCKEDELVRDSPARKAVSRYLRCLSQAIGWSVTLLLIIAAFLARCLRPCFDQTVFLQRRYWSNYVDLEQKLFDETCCEHARDFAHRCVLHFFASMRSELQARGLRRGNAGRRLELPAVPEPPEGLDSGSGKAHLRAISSREQVDRLLSTWYSSKPPLDLAASPGLCGGGLSHRAPTLALGTRLSQHTDV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should contain a Helical membrane-spanning region.2. For general function, the protein need meet that CALHM1-CALHM3-mediated ATP released acts as a neurotransmitter to gustatory neurons in response to GPCR-mediated tastes, including sweet, bitter and umami substances.; Pore-forming subunit of a voltage-gated ion channel, also permeable to larger molecules including ATP3. The ideal protein would be one that plays a critical role in sensory perception of taste, ATP transport through cation channel activity, voltage-gated ion channel activity within basolateral plasma membrane. The designed protein sequence is ",
    "response#1": "MVTFITATLWIAVFSYIMVWLVTIIGYTLGIPDVIMGITFLAAGTSVPDCMASLIVARQGLGDMAVSNTIGSNVFDILVGLGVPWGLQTMVVNYGSTVKINSRGLVYSVVLLLGSVALTVLGIHLNKWRLDRKLGVYVLVLYAIFLCFSIVT",
    "response#2": "MVTFITATLWIAVFSYIMVWLVTIIGYTLGIPDVIMGITFLAAGTSVPDCMASLIVARQGLGDMAVSNTIGSNVFDILVGLGVPWGLQTMVVNYGSTVKINSRGLVYSVVLLLGSVALTVLGIHLNKWRLDRKLGVYVLVLYAIFLCFSIV",
    "response#3": "QGFGELFEKAKLNNNNRKISNGDNSLFFSNFSLLGSPVLKDINFKIEKGQLLAVAGSTGAGKTSLLMMILGELEPSEGKIKHSGRISFCSQFSWIMPGTIKDNIVFGVSYDEYRYKSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKQIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLRPDFSSKLMGYDSFDQFSAERRNSILTETLRRFR"
  },
  {
    "reference": "MNEQGYPRTPPGDTGPGERQEARFGFEPVPASEKQARVNAVFSRVADKYDLMNDLMSGGLHRLWKDELIGWLNPPRGNITYRLVDVAGGTGDIARRFLAAAGARAECLICDISGEMLKAGRTRLQPPPAGLSFIQANAESLPLADASCDAYTIGFGIRNVTHMERALSEAWRVLRPGGRFLCLEFSQVQVPILDSLYERYSFGAIPALGGLVAGDAASYRYLVESIRNFPDQDSFADMVRAAGFERVSYRNLTGGIAAIHSGWRL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a high affinity for S-adenosyl-L-methionine.2. We need a protein that can perform a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine with high efficiency.3. A protein with 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity, S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity, S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity, demethylmenaquinone methyltransferase activity is needed to enhance ubiquinone biosynthetic process, menaquinone biosynthetic process, aerobic respiration in a specific context.4. The protein should be optimized for activity in menaquinone biosynthesis, Quinol/quinone metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MNNEFEKIEDALKDIKKGKMIIIVDDENRENEGDLVIAAEKISPQAINFMIKEGRGLICIPMEGERLEELKLKPMVENNTDSHKTAFTVSIDAKETTTGISAFERAFTIKKIVEPNAKPEDFRKPGHIFPLIARKGGVLRRAGQTEAAVDLARLAGLYPAGVICEIMNEDGTMARMPELIKFAKKHKLKIITIEDLIKYRMKKEKMVYEAYDLMNDLMSGGVHRLPIEIKPKKHNEFYLATKKAKLGHMLHFNDHELNVYLQAKKEKMIHYSKAIKRNKYLHTK",
    "response#2": "MSNRYFGTTGVRGLVPDLDPVVAARLARAFATELGGRPGGDVVVGRDTRPSGDALVAAVAAGCRAAGADVVDVGLAATPALAYAVARLGGAAGVVVTGSHIPADRNGLHFYSARGEIRKSDEERLADAIALPDGAGGVPTGPVGEHAERVREEFAGRIAPARRLRVVLDCGNGAAGPAGPELLRRLGHEVVAVHGDVDGRFPGRHPDPTEPGNLEGLCRCVREAGADLGVAFDGDADRCLVVDAEGREVDGDFLLALVAAEHPGPGGGGGPVVYDVKSSQALPEVVRAAGGRPERSRVGHAFIKERMRADGAALGGELSGHFFYAERWFGFDDAIYAGVRLLELLGRDGRPLSELTAGFPRYPSSPERLAVPDERKFAVIEELRAAVGGGEVDTIDGLRVDFPDGWGLVRASNTQPALVLRFEAADEETLRRVRDLVVERLSAVRPDVAL",
    "response#3": "MLQKKTENQKIQTLKDIAGLSGIKGISSGSREVAEGDVFFAYHGDTNDGRDYIPQAIEKGAVAIVSEQDYPWDIPVIQVRNVRALMAKIASRFYNEPQQKLKIIGF"
  },
  {
    "reference": "MEKIKVKIKGKEYEVEKGTPLGKIFELAGIKDALGGVINGKIIDLQTPVRESGEIKPVYRGSKESLEIMRHSLAHIMAQALKELYGAKKVHLGVGPTTEEGFYYDVEVEGHKITEEDLPKIEQKMKEIIERDYPILRRELSREEAIKLFDKLKEKYKIDIIKEIPEEEVISVYEQGDFIDLCKGPHLPSTGKAGAFKLTSISGAYWKGRSDQPQLTRIYGIAYWSDKEVKERLKFYEEVKKRDHRRLGKELEFFTIDDNVGAGLILWLPRGAIYRKVLEDYLREEHLKRGYQLVYTPHVGKSKLWETSGHLECYKQNMFPSMKIDEEEYYVKPMNCPFHIAIYKSRTRSYKELPLKLFELGTVYRYELSGVLHGLLRVRGFTQDDAHIVCTPEQVNDVIRETLDFALSTLKDFGFNEFKIYLSTRPEYSIGSDEQWEVSQNALKKAIEDLGYEYEIDEGGGAFYGPKIDVKIRDAIGRMWQLSTIQFDFNLPERFDMTYVGPDNKKHRPYMIHRALLGSIERFTGILLEHYAGLLPIWLSPTQVMIIPIADRHHEYAKKVYEFLKENGIRAEMDLREERMNAKIRDAELKKIPVILVVGDREAQNNTVSVRTKKEGNLGSMELNKFLDWIKEKIKNKE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should be able to enhance the efficiency of threonyl-tRNA aminoacylation. The designed protein sequence is ",
    "response#1": "DNPVAKTWGSKPELDFEPKPHWEIGEALGILDFERAAKIAGARFALYWKMGAALERALIAFMLDIHTKRHGYTEVLPPFIVNSTSLLGTGQLPKFKEDLFKLEDRDFYLVPTAEVPVTNIHMNETLEESELPKLYTAFTPCFRSEAGSYGKDTRGLIRQHQFNKVELVKLVKPESSYEELEKLLLDAERILQELGLHYRVVTLCTGDMGFSSAKTYDIEVWLPGQNTYREISSCSNFEDFQARRANIRFRRKGQSKTEFVHTLNGSGLAVGRTLVAILENGQQADGSVSIPPALRPYLG",
    "response#2": "DNPVAKTWGSKPELDFEPKPHWEIGEALGILDFERAAKIAGARFALYWKMGAALERALIAFMLDIHTKRHGYTEVLPPFIVNSTSLLGTGQLPKFKEDLFKLEDRDFYLVPTAEVPVTNIHMNETLEESELPKLYTAFTPCFRSEAGSYGKDTRGLIRQHQFNKVELVKLVKPESSYEELEKLLLDAERILQELGLHYRVVTLCTGDMGFSSAKTYDIEVWLPGQNTYREISSCSNFEDFQARRANIRFRRKGQSKTEFVHTLNGSGLAVGRTLVAILENGQQADGSVSIPPALRPYLG",
    "response#3": "DHRKIGKTLDLFHWQEEAPGMVFWHNDGWTIYTELEKFIREKLHEYDYDEVKAPMMMDRSLWEKSGHWDKYADGMFTTTSEKREYAIKPMNCPGHVQIFNQGLKSYRDLPLRIAEFGCCHRNEPSGSLHGLMRVRGFTQDDAHIFCMESQVQAEVKKCIEMVYDVYGSFGFEDVVVKLSTRPDNRIGSDEIWDKAEAGLAQALTDSNIAFEYLPGEGAFYGPKIEFTLMDCLGRAWQCGTVQLDFALPERLGATYVGEDNERYTPVMIHRAILGSLERFIGILIEEFTGKFE"
  },
  {
    "reference": "MKLIKTPDRIGSMFNRIAPVYDFLNHFLSVGFDIYWRSQATKALVSELKCKPAMVADVASGTGDLALSLMKRETWLFVVGIDIAEKMLKKGKQKLQKEGYDNRFLPVLGDALNLPLSGSSCDAIMIAYGIRNVLNVEKALEEFARVLKPGGLLLILEFSLPKAKLFRDVYLFYFKKILPTLGRLVSGDIDAYSYLPTSVTAFMTVEDMEMMLQNAGFKVMETRCFLSGVSYYILGKYKGR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity, S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity, S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity functions is required to enable aerobic respiration, methylation.2. The desired protein should be able to perform a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine3. The protein design should take into account the physiological conditions of the menaquinone biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MSEHRLDRRIKGVFFDLDDTLMDTSGAIRRAAEETFRAVAAELGLSLDDSDLAEVNDRINRVFWDRYQRDELSFEQRRYRRFADVFSLLGVEQPEDLLEEFIDAYHQAWRQVSLFPDTVPMLERLSAEFPLGLITNGRGDYQIAKLKALGLDRYFPAEKFVFSGEVGVAKPHPLIFEEAIAGLGLSPAECLMVGDNLEADVLGAQNAGCRTCWLNRGHEPLPAEKTPQPDYIIDHLAELLPLITGN",
    "response#2": "MNASLQGKVALVTGSGRGIGAAIAVRLAAAGAKVVVNYAGSEEEAKDVVARIEAEGGRAVAVQADIGKREDVRRLFAEAEAAFGGIDILVNNAAIWDFSPLEAVDEDDWDRLFAVHVKAPFFLTQEAVKLMRERGGGRIVNIGYDVMNDILSFGLHRGAQYNASKGALLGLTRSMARALAPHQITVNAVAPGFIVSDMTENLSDEWRARIAAGVPLRRIGEPDDVAGPVAFLASADAGFVTGQVLHVNGGMLM",
    "response#3": "MSNAAPHEKGFHIXSPEDLAXLKEELRFPASDGYDLMNDLMSAGMHRAYRAWMVGERRGRNVLDLCSGTGDLSFLAAPLVGPSGAVIGVDPSKAMLDIAREKAAAAGATNVDFRVGAAEALPFPDNSFDCVSIGFGLRNVTDKDAALRSMWRILKPGGRLLVLEFSKPVMPLLSKAYDWYSFTLLPKIGKLVAGDADSYQYLAESIRMHPDQETLKGMMLDAGFGHVDVHNLTGGVVALHVGFKF"
  },
  {
    "reference": "MAGNAKLLHETLWDQDPELMDLIRKEKTRQIRGLEMIASENFTSLSVLQCLSSCLHNKYSEGLPGQRYYGGNEFIDQIELLAQKRALEAYRLSPEEWGCNVQPYSGSPANFAVYTALIEPHGRIMGLDLPDGGHLTHGFMTQTKKISATSIFFESMPYKVDPVTGLIDYDKMEETARLFKPKVIIAGISCYSRCLDYKRFREIANQNGAYLFADMAHISGLVAAGVIPSPFEYADVVSTTTHKTLRGPRAGVIFFRKGVRTVKANGEKVMYDLESRINQAVFPGLQGGPHNHAIAGIATCMLQAQSPEFRAYQEQVIKNARALCAGLLEKGYSVATGGTDVHLVLVDLRPVAITGARAEYILEEISIACNKNTVPGDKSALNPSGIRLGTPALTTRGLLEKDMQQVVEFIDRGLRLSKEIANVSGPKLSDFKRILHEDSTFSEKVNNLRKEVEQYSEQFLLPGYADF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be involved in folic acid metabolic process, one-carbon metabolic process, tetrahydrofolate interconversion, tetrahydrofolate metabolic process, protein homotetramerization by localizing to nucleus, cytosol and exhibiting mRNA regulatory element binding translation repressor activity, serine binding, glycine hydroxymethyltransferase activity, protein homodimerization activity.2. The protein have enhanced (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O =   (6S)-5,6,7,8-tetrahydrofolate + L-serine.3. The designed protein have Serine hydroxymethyltransferase-like domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "YDTDPEVYSGVRAEDRRQEENLELIASENMASRAVRQAQSSRMTNKYAEGYPGKRWYNGCECVDEIERLAIDRAREIFGGEHVNVQPHSGSAANMAAYFSVLEPGDTMLAMSLAEGGHLTHGHPMNFSGRFFNIVPYGVDEKTEQIDYDRLAELASEHKPKMIVAGASAYSRLIDFKRLRAIADDVGAYLTVDMAHIAGLVAAGCHPNPVPYAEFVTTTTHKTLRGPRGGMVLCREAFAKEVDRQVFPGIQGGPLMHTIAAKAVCFHEALQPEFKTYQQQVVRNAQELAAALQEQGIRIVSGGTDNHLMLADLTSVEISGKDAANALDKASITVNKNSIPFDGKSPFVTSGIRLGTPAVTTRGMGPEQMREIAGLIAEVLDATHDPEKRANVGERVAALCDKFPVYR",
    "response#2": "LQKQDKEVFDAIRLELGRQRNNIELIASENFVSEQVMEAMGSVLTNKYAEGYPRKRYYGGCEYVDIVEDLARDRAKQLFGAEYANVQPHSGAQANMAVYQAVLKPGDTVLGMNLAHGGHLTHGSPVNFSGLLYNFVEYGVSKETKQIDYDIVRETALAHKPKLIVAGASAYPRAIDFAKFREIADEVGAYLMVDMAHIAGLVAAGLHQNPVEYADFVTTTTHKTLRGPRGGLILAKAEWETKINKAIFPGIQGGPLMHVIAGKAVAFGEALQPEFKNYAAQIIKNSKQLAKTLEENGVQVLTGGTDNHLLLIDLNHLSITGKAAEEALDSVGITVNKNSIPFETESPFVTSGIRVGTAAITTRGFDEAATQIVGELIAGMLPKADTTALAAQRRQDPALAEILLGELDRQSTTLQLIAAENFTSPAVLAALGSPLANKYAEGYPGARYHGGCEIVDVAERLAVQRAKRLFGAEHANVQSHSGSSAVLAAYAALLRPGDTVLALGLPYGGHLTHGSPANFSGRWFDFVSYGVDEETGLIAYDQVRTLARTHRPKAIVCGSIAYPRHLDYAYFREVADEVGAYLIADAAHPIGLVAGGAAPSPVPYADVVCATTHKVLRGPRGGMLLCGADLAERVDRAVFPFTQGGAQMHTIAAKAVAFGEAATPAFQAYAHQVVANARFLAAALAAEGLAITTGGTDTHLITADPAPLGVDGRIARGRLAAAGMILDSCALPHDDARGLRLGTAAVTTQGMGEGEMARIAKLAEADAKVAEAIKAELERQQSQIELIASENIVSKAVLEAQGSVLTNKYAEGYPGRRYYQGCAPSDAIETLAIERAKQLFGCGFANVQPHSGAQANGAVMMALLKPGDTILGMSLAAGGHLTHGAPPAQSGKWFDAVQYGVRQDDHLVDFDEVERLAKEHRPKLIIAGGSAYPRHLDFGRFRAIADEVGAILMVDMAHFAGLVAAGEHPTPFGHAHVVTTTTHKTLRGPRGGMVLTDDEAIAKKINSAVFPGLQGGPLMHVIAAKAVAFGEALEPGFKTYAKAVIANAQTLAMRLKERGADLVAGGTDTHLALVDLRPLGLTGKDADTSLEHAGITCNKNGVPFDPLPPMQTSGIRVGSPAGTTRGFGEAEFRDIADM",
    "response#3": "LETCDANIARLISRERFRQEIHIELIASENYVSRAVLEAQGSVLTNKYAEGYPGRRYYGGCQVVDEIESLAIERACALFGAEYANVQPHSGSQANQAAMLALLQPGDTLLGMALADGGHLTHGAKVNFSGKWFNAVTYGVNPESETLDYEQLERLARTHRPKLIIAGASAYARPIDFARFRALCDEVGAYLMVDMAHYAGLIAAGLYPSPVPYADIVTSTTHKTLRGPRGGLILARQEHAAALDKAIFPMYQGGPLMHAIAAKAVAFHEADTEDFRRYQAQVIENAKGMAQVLTERGLRIVSGGTDCHMFLVDLQPLGLNGKAAEALLESARITLNKNAIPNDPQAPTITSGIRIGTPAITTRGMGLEQARLLAQLIAEGIEHPLNGETLERRQDPALAEILLGELDRQSTTLQLIAAENFTSPAVLAALGSPLANKYAEGYPGARYHGGCEIVDVAERLAVQRAKRLFGAEHANVQSHSGSSAVLAAYAALLRPGDTVLALGLPYGGHLTHGSPANFSGRWFDFVSYGVDEETGLIAYDQVRTLARTHRPKAIVCGSIAYPRHLDYAYFREVADEVGAYLIADAAHPIGLVAGGAAPSPVPYADVVCATTHKVLRGPRGGMLLCGADLAERVDRAVFPFTQGGAQMHTIAAKAVAFGEAATPAFQAYAHQVVANARFLAAALAAEGLAITTGGTDTHLITADPAPLGVDGRIARGRLAAAGMILDSCALPHDDARGLRLGTAAVTTQGMGEGEMARIAKDLEGARVRQMLDYVTCADLSALKEGESTAVCLETPEGHVEGALTCVTWDHYRLSLPAKQAAATVTWLRDLSDGYVSLRLDGAPDYSAMRIPG"
  },
  {
    "reference": "MGGKWSKSSLRGWPQVMERIRRTPVRQPPAAAEGVGPASQDLARHGAITTSNTAENNPDSAWLEAQQEDSEVGFPVRPQVPLRPMTFKAAFDLGFFLKEKGGLDGLIYSKQRQDILDLWVYNTQGYFPDWQNYTSGPGTRYPLTFGWPFKLEPIDPAEVEEANKRENSCLLHPISQHGMEDEDKEVLVWRFDSSLARRHIARELHPEYYKDC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that contributes to suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host autophagy by functioning in host cell Golgi membrane, virion component with GTP binding.2. For general function, the protein need meet that Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK13. The protein design must feature the Diacidic; necessary for CD4 internalization, Dileucine internalization motif; necessary for CD4 internalization, PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif. The designed protein sequence is ",
    "response#1": "MGGKWSKRSGAGWSTIREKMRRAEPAAEGVGAASRDLEKYGAITSSNTPATNAACAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRYPLTFGWCFKLVPVDPEKVEEANEGENNCLLHPMSQHGMEDPEREVLMWKFDSRLAFHHVARELHPEYYKDC",
    "response#2": "MGGKWSKRSIVGGWSAVRERMRRAEPAAEGVGAVSRDLERHGAITSSNTPATNADCAWLEAQEDEEVGFPVRPQVPLRPMTYKAAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPSEVEEANEGENNSLLHPMSLHGMEDPEKEVLEWRFDSRLAFHHMAREKHPEYYKDC",
    "response#3": "MGGKWSKRLPAVRERMRRAEPAAEGVGAVSRDLEKHGAVTSSNINHPSCVWLEAQEDEEVEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWIYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEQEKVEEATEGENNCLLHPMNQHGMDDPEREVLMWKFDSRLAFHHMARELHPEYYKN"
  },
  {
    "reference": "MPQQTAVVVLAAGAGTRMRSKTPKVLHPMAGRSMLAHALHAASEIDPTYLITVIGHDREAVGDAVGKVGAELGRQIILAVQEEQRGTGHAVQCALTALPEDFSGDVLVTYADVPLLDGHTLHALLDEHRSYTEGSAVTVLTFAPDDPNGYGRIVRDPQGRLLEIVEHADATPEQAAIGEVNSGVYVFDAAVLRVMITRIDTANARYELYLTDVLKLAREAGHPVHGARLLDSAKVTGVNDRVQLADAARTLNRYIVERHMRAGVTIIDPATTWIDGSVVIGRDAVIRPGVQLLGDTSIGEDAEIGPDSTLTDVVVGDGAKVVRTHGEKANIGPGANIGPFAYLRPGTSVGEAGKLGAFVETKNANIGAHSKVPHLTYVGDATIGEHSNIGASSVFVNYDGVRKHHTVVGSHVRTGSDTMFVAPITVGDGSYTAAGTVLRRSVPPGALAVSGGAQRNIEGWVQRNRPGTDAARAAESALAGNSDEPSKTEISNEQKDGERP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein should be able to manipulate the activity of enzymes in the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway, either by activating or inhibiting their function.2. The designed protein should be able to compete with endogenous proteins or inhibitors for UDP-N-acetyl-alpha-D-glucosamine, Mg(2+), acetyl-CoA binding.3. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.. The designed protein sequence is ",
    "response#1": "LVLAAGAGTRMKSDTPKVLHTLAGRSMLSHSLHAVAKVAPQHLVVVVGRDRERVAPAALEIGQELGRPVEIAVQEQQQGTGHAAECGLSALPDDFTGVVVVTAGDVPLLDADTLADLIATHSAEAAVATVLTTTLADPTGYGRVLRTQDREVIGIVEQADASPSQQAISEVNAGVYAFDIAALRSALSRLRSDNAQHELYLTDVISIVRSDGEVLGIVEHRDASEAERAIREINSGVYAFDAAVLRDALGKVRTDNSQGEEYLTDVLGILREAGHRVGASVAGDHREILGINNRAQLAEARRLLNDRLLRSAMLASSVGANCHIGPYARLRPGSQIHDGAHIGNFVEIKGTDSVVENNVKIGPFAQLRPGTTIREGVKIGDFVEIKGVTIVDPASTWVDVDVRIGRDTVVRPGTQLLGATHIGGRCTIGPDTTLTDVTVGDGAEVVRTHGSSSVIGDDAVVGPFTYLRPGSVLGVAGKLGAFVETKNATIGDGTKVPHLTYVGDADIGEHSNIGASSVFVNYDGEAKRRTTIGSHVRTGSDTMFVAPVTIGDGAYTGAGTVIREDVPPGALAVSAGPPAVVGAGTEIGPNSRIDADSGVKIGADTVVYPGAHIKGGSIIGESVVIEEGARVVNSTIGARSKVLVLAAGAGTRMKSDTPKVLHTLAGRSMLSHSLHAVAKVAPQHLVVVVGRDRERVAPAALEIGQELGRPVEIAVQEQQQGTGHAAECGLSALPDDFTGVVVVTAGDVPLLDADTLADLIATHSAEAAVATVLTTTLADPTGYGRVLRTQDREVIGIVEQADASPSQQAISEVNAGVYAFDIAALRSALSRLRSDNAQHELYLTDVISIVRSDG",
    "response#2": "VIILAAGKGTRMHSDLPKVLHEVAGKAMVELVLDTSKAAGAQKIVTVIGHGAQAVRDRLGDQTEFAEQKEQLGTAHAIQMTEPLLGDVEGTTIIMSGDAPLFTVETINGLFDFHAQSGNAVTVLTATAPDPTGYGRIVRDENNNVTKIVEQKDANELEQAIKEVNTGVYAFDNQLLFAALKKVKNDNAQGEYYLPDTLEILREDNHTVGAFVMADWDETLGVNDRVQLARAEKVMRQRINEQHMRNGVTMIAPDTCYIDSGVKIGSDTIIHPNVHVSGNTIIGSECVIEPSVVIRNCTIADKVTLKAGSVMADSVIGEEAAIGPMAHLRPDSQLGAHVKIGNFVETKKIVMGEGSKASHLTYLGDASIGRDVNIGCGTITCNYDGVKKHRTVIGDGVFVGSDVQFVAPITVGANSLIAAGTTVTQDIPPDSLALARVPQVNKAGWVKRRGKQQ",
    "response#3": "MSLVLAAGAGTRMKSDTPKVLHTLAGRSMLSHSLHAVAKVAPQHLVVVVGRDRERVAPAALEIGQELGRPVEIAVQEQQQGTGHAAECGLSALPDDFTGVVVVTAGDVPLLDADTLADLIATHSAEAAVATVLTTTLADPTGYGRVLRTQDREVIGIVEQADASPSQQAISEVNAGVYAFDIAALRSALSRLRSDNAQHELYLTDVISIVRSDGAQHERTVIEDDAFIGSDTQLVAPVRVGKGATIGAGSTILGVNDRSQLAEAERALRRRINTEWMLAGVTLADPARIDVRGTLTCGRDVEIDVGCIFEGAVSLADDVRVGAYSIIRNASIAQGTQIAAYSHIDSSSVGANCHIGPYARLRPGSQIHDGAHIGNFVEIKNSAIGQGSKANHLSYIGDATVGSRVNIGAGTITCNYDGANKFRTVIEDDAFIGSDTQLVAPVRVGKGATIGAGSTITQNAPDGVLTLSRSKGVTMIAPDTCYIDSGVKIGSDTIIHPNVHVSGNTIIGSECVIEPSVVIRNCTIADKVTLKAGSVMADSVIGEEAAIGPMAHLRPDSQLGAHVKIGNFVETKKIVMGEGSKASHLTYLGDASIGRDVNIGCGTITCNYDGVKKHRTVIGDGVFVGSDVQFVAPITVGANSLIAAGTTVTQDIPPDSLALARVPGVPAHVKRKG"
  },
  {
    "reference": "MRVLGIESSCDETGVAVYDTGLSGSAALRAHAVYSQIALHAEYGGVVPELASRDHVRKLLPLVRQTLAEAGLGVGDIDGVAYTAGPGLVGALLVGAGVARSLAWALEVPAVGVHHMEGHLLAPLMEDDPPEAPFVALLVSGGHTQLVAVDAIGQYRLLGETLDDAAGEAFDKTAKLMGLPYPGGPQLARLAEQGTPGAYRFARPMTDRPGLDFSFSGLKTQVLMAWRDSDQSEQTRADIARGFEDAVVETLSIKCERALEAAGTNVIVVAGGVGANKRLRARLQQMAERLGGRACFPRPALCTDNGAMIAFAGALRLQAGQYSPPKVDVTPRWDMATLPAV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must be engineered to have a high affinity for Fe(2+) in order to increase its catalytic activity.2. The designed protein must be able to regulate tRNA threonylcarbamoyladenosine modification. The designed protein sequence is ",
    "response#1": "MRVLGIETTCDETAAAVVRLEPGGAGEILSNEVMSQIAEHAAYGGVVPEIAARAHIDIIDRLVLRALAHAGTRLADLDGIAAAAGPGLIGGVIVGLTTAKALALASRKPFIAVNHLEAHALTARLTDALEFPYLLLLVSGGHTQLVAVRGVGDYLRLGSTVDDAVGEAFDKIAKMLGLPYPGGPEVEKRAAEGNAARFDFPRPMLGRPKPDFSLSGLKTAVRLEMERLNSLSPTDVADLCASFQAAVVDMIIDRSRVGLRLFRETVGPSNAMVVAGGVAANGAIRRALMRFCGESGMRLVLPPPQLCTDNGAMIAWAGLERLSLGLIDDLTFAARARWPLDAL",
    "response#2": "MLTLGLESSCDETSCALVENGKILANRIASQDIHAAYGGVIPELASRAHLQIFPKLLAAVAQDAEVSLEDVELISVANTPGLIGALSVGVNFAKGLASGLKKTLIGVNHVEAHLYAACLEEPSIRFPALGLAISGAHTSLFLMPNATTFLLIGKTRDDAIGETFDKVARFLGLPYPGGQKLEELAQDGDEEAYPFSRAKVSGNDFSFSGLKTAVLYALKGNNSSAKAPFPEVSETQKRNIAASFQKAAFMTIAQKLPDIVKAFSCESLIVGGGVANNRYFRRLLNQTCSLPTYFPSSQLCSDNAAMIAGLGERLFCNAEP",
    "response#3": "ELLANIIASQVESHKRFGGVVPEVASRHHVEVVTTCFEDALQEAGIVASDLDAVAVTYGPGLVGALLVGMAAAKAFAWANKLPLIPVNHMAGHLMAARDVKELQYPLLALLVSGGHTELVYVSEPGDYKIVGETRDDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMIKEDHLEFSFSGLKSAFINLHHNAEQKGEALVLEDLCASFQAAVLDILLAKTQKALLKHPVKTLVVAGGVAANQGLRERLATDISPDIDVVIPPLRLCGDNAGMIY"
  },
  {
    "reference": "MPISANKYFLLVGVFIGDKMKKEAAKLNEDYVVENAAIQRLKNLGYSYKHGSELTPEYNERESYRDAILKNRFIKAIKNINPWLTEELALKVYKTVTNIDNPDFNMRGKIFYEMLINGVKLEFKENGEKKTRFVKLIDFENINKNEFLVANQFEVEYYYENGRFRRPDLVVFINGIPIAIFEFKSPKSNQTAKDAFNDHKTKMKDIPQLYQYAQILVVSDGLETKYGSPTSDWDRFFVWEGVESDDDVEVIEVDNYGNTMYKYKGNPYTSLDILLMGLFKKEHLIEFLEDFIIHDKKKIIATYYQFYTVKKAVDRTIKSVLYGETPEDRRIGIVWHAQGTGKSITMLFYAKKALKQKELNYPLLVFLTDRLELDEQLYNVFSSVFSEAERAESIAELQELIKKTPGGIIFATIQKFGRKSKDEHYPFLTDRNNIIIIADEAHRSHYGTLAQNLRKAIPNASFLAFTATPIDYKDRSTFLVFGDYISAYPIDKAKRHGVVVPIYYEARLVELHLTNEFIDLEFDEISERVANDPETKESIKEVFAKLEKIMLTEDYLSKVSKDIIEHFNKRLQDFDGKAMVVTISRKVAVELYKWITKQPNAPKIAVVMSGNKSKDPEDFHPHIRTKKELENLAKEFKDPESDLKMVIVVDMWLTGFDVPCLHTMYFLKPMKNHSLAQAIARVNRVFKDKPGGLIVDYIGIADDLSKSLSKYSSEARKDLMTDIKVVIEEMKRRYEKVTSYFKNINYKDWKKLSSEDLSLLTVKAYQRV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should include a DEAH box motif to ensure proper function.2. For general function, the protein need meet that The R subunit is required for both nuclease and ATPase activities, but not for modification; The restriction (R) subunit of a type I restriction enzyme that recognizes 5'-CCAN(5)GTR-3' and cleaves a random distance away; After locating a non-methylated recognition site, the enzyme complex serves as a molecular motor that translocates DNA in an ATP-dependent manner until a collision occurs that triggers cleavage.3. The protein should be designed to have high stability and activity towards Endonucleolytic cleavage of DNA to give random double-stranded   fragments with terminal 5'-phosphates, ATP is simultaneously   hydrolyzed..4. The designed protein should contain one or more Helicase C-terminal, Helicase ATP-binding domains that are essential for its function.5. The protein design should be able to enhance the efficiency of DNA restriction-modification system. The designed protein sequence is ",
    "response#1": "MKFNPILQGECPEIQGIDFDAKKETVEQRHQAVIEAIDKVKALWRHHCNEIADWITSSGVDKRSYSSRFLPKWIDEITAWATPLETKSYQLPDCLSRFSQATLHEKTTKGAAPEHALFAEIELLCQQSLTLRDVIIANAIPEIRQGIENEKMRRGELGFDDLLTRLDKALQREGGEALADAIRQRYPVAMIDEFQDTDPQQYRIFDTIYGEHTESGLLFIGDPKQAIYAFRGADIFTYIRARQQTDHHYTLNTNWRSLDLSAISLPIFLPFSPTMAQERVIAEINADLVRHRPMRRLLQGDVGSGKTLVAAAATLTALEAGYQVAMMAPTEILAEQLHARFQQWLEPLGLEVGYLVGSRSPRARRETAETLAGGSLRLVIGTQSLFQEGVVFACLGLVIIDEQHRFGVEQRRQLLEKGAMPHLLVMTATPIMVEDGITVSGILLFGRTPNRFLPQEKIAKILEERGIKAKAYHAKTPFSVFEAFINGELDVLVGVANYYGALVRGIDLPERVKHAVFIGVPRFKFPVTVGEPHPANLMRLLSLLVDIDIEDVARQARGYLAEVRRIVKRYSPAYLQYLTEKILEGEIEGENRTLRVLIEAYKFLLNALSDKDVWDRLRDRSDIATIEYNGLKYILLPDISTYIQASGRTSRLQRVWAKRVFMNQSFAIVAPTGVGKTTFGLVMSLFLKGRVLAIFPTRLLAQQAGDRLSELAQKVGVNKKILIYQSKKNIREQFLNGDWDILLGTNMFLHKNFENLINFKFKLIFIDDIDSFLKRGKNVDYLFKL",
    "response#2": "MRAGPVGPQRSWIVRLAKGESFSIIAPPGLGKTTFGIISSVYFATKKQKRSLLMFPTKTLVNQVKQKIQEVAVNTSSNIKLLVYDSSMKQSQRSEFMKSLQAEDFDIALLSSRFAMMNLHTLTTLDFGFLFVDDVDSALKSNKSAMAVTMLAGYSEEDISRVKELLRISYRDPSAFSKIAELRRKDKIVVFSSATVNRSNPVLSSLMGFKPGSSNIYLRKVIDSFTNLPELNRFIKEAQYSAIFTVQSIPALVKYYDAFKKISKDYEHPLKVAGVFSYAANEERNEGEVDEAHSRGKLEEVIQDYNLNFGTNFSTDTFQEYFNHISKNVKKGVKDNKIDVLIVVNMFLTGFDSKVLNTLYVDKNLKYHDLIQAYSRTNRVEKETKPFGKIVNYRI",
    "response#3": "MKKTMWSIQRSWARRLLQWDSFAIVAPTGVGKTTLLIIYALYRAIQNYKIYIIVPTKEIAKQIALRMNQYVKELHRELNIVAYLPSNNNERQELLKKIQSEDVNVLITTAAFLAKNYNVLKNIEFDIVIADDFDAILRKSKSINKVLNLLGFSEELIKKAFRIVTLRSEILRLRLLGFDAENLQKLVTTLNELEYLPFSPTMAQERVIAEINADLVRHRPMRRLLQGDVGSGKTLVAAAATLTALEAGYQVAMMAPTEILAEQLHARFQQWLEPLGLEVGYLVGSRSPRARRETAETLAGGSLRLVIGTQSLFQEGVVFACLGLVIIDEQHRFGVEQRRQLLEKGAMPHLLVMTATPIMVEDGITVSGILLFGRTPNRFLPQQWDSFAIVAPTGVGKTTLLIIYALYRAIQNYKIYIIVPTKEIAKQIALRMNQYVKELHRELNIVAYLPSNNNERQELLKKIQSEDVNVLITTAAFLAKNYNVLKNIEFDIVIADDFDAILRKSKSINKVLNLLGFSEELIKKAFRIVTLRSEILRLRLLGFDAENLQKLVTTLNELEYELKRKLLQCRKSQLIVASATGRSSSLRSKVIRELLGFEVGLGSPPWGAQRTWIKRLVRGDSFSIVAPTGTGKTTFGIVAALYLACVKKAKSYILVPTTTLVAQIVEKANTLAKNIGCTPIVVGFHSKMKRSERENALNMIMSGGFDILVSTIAFARRYADILSNYKFKFVLVDDVDAVLKSGRSIDAVLKIVGFSRDHLEKATKLLSLQREIARILESASEEEFREKSSIKSKLANIQNDIEAYKRELLEMRGKVAQVIFSSATGRPRGSRVRLFRALLGFEVGGRGDIGLRNIVD"
  },
  {
    "reference": "MTKYIFVTGGVVSSLGKGITAASLGRLLKNRGFSVTVQKFDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFMDINLTKYSSVTTGKIYSTVLRKERRGDYLGGTVQVIPHITNEIKERVFRAGHETNADVVITEIGGTVGDIESLPFLEAIRQIKSDIGRDNVMYIHCTLVPYIKAAGEMKTKPTQHSVKELRSLGIQPNIIVVRTEMPMTQDMKDKLALFCDIDEKAVVEAQDADTLYAVPLALQAQNMDQIVCDHLKLESKTEADMTEWRGLVDKVRNLSKTTKIALVGKYVELQDAYISVVEALKHAGYNFDADVQVKWVNSDEVNAENVEELLGDVDGILVPGGFGDRGIEGKILATRYAREKKKPFFGICLGMQLATVEFARHVLGFEGAHSAEIDPETKHPIIDLLPEQKDIDDLGGTLRLGLYPCKLTEGTKAFDAYKDEVIYERHRHRYEFNNHYRPAMEKAGFVFSGTSPDGRLVEIVELKDHPWFLACQFHPEFTSRPTRPQPLFRDFVEAAIKVMNE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be optimized for activity in CTP from UDP: step 2/2, Pyrimidine metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The Nucleophile, active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The protein should be able to bind Mg(2+), UTP ligand in a variety of conditions4. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen5. The protein design must be able to perform ATP binding, metal ion binding in order to contribute to glutamine metabolic process. The designed protein sequence is ",
    "response#1": "DAYLSIHESLKHAGLDAATEVETTWIHSEELVDGVGDAFEGIDGIVVPGGFGSRGTEGKIAAIRYARENDVPFLGLCLGFQMAVVEFARNVLDLDGAHSAEMVENTAHPVIDLLPEQEDVDDMGATMRLGAHETQIEAGTLAHALYDSTTCTERHRHRYEVNPEYIPDLEAAGLTFSGESGRRMEILEIDDHPYFLGTQFHPEFRSRPGRASPPFVGFLG",
    "response#2": "RDSYKSLSEALTHAGLQTRTRVNIRYIESGQVEGDDLSCLADVDAILVPGGFGDRGFEGKINTVRYARENQIPYLGICLGLQAAVIEYARHVAGLEGAHSTEFDPATEHPVIALITEWQDMDGTSKERSMESNLGGTMRLGAQECRLASGSLMATSYGNESIFERHRHRYEFNNQYLERLQQAGLTFSGFSIDGLVETIELRDHPWFVACQFHPEFTSNPRDGHPLFTGFIVKVAIVGKYTQLEDAYKSIAEALTHGGMANRVKVRIEWVDAELFDKEDATPYLQGFHAILVPGGFGERGTEGKIKAAQYAREHKVPYLGICLGMQMAVIEAARNVAGIEEAGSEEFDHEAGKKRFEPVVYHLKEWVQGNHKVSRSADDDKGGTMRLGAYDAALTEGSKVAEVYGSNAIEERHRHRYEVDIKYREQLEACGLKFTGMSPDGRLPEIVEWTDHPWFIGVQFHPELKSKPFDPHPLFKDFLQAA",
    "response#3": "LPDAYLSVTEAMRAGGFQNDAKVHIRWVASDECQTEAGAQRALGGVDAVLVPGGFGVRGIEGKLGALRWAREKQVPTLGICLGLQCMVIEYARNVAGIEGASSTEFDPQTPAPVIATMEEQKAFVEGAGDLGGTMRLGAYPAKLAEGSVIAEAYGAVAVDERHRHRYEVNNGYRKELEEAGLVFSGLSPDGNLVEFVELPREVHPYYVSTQAHPEFKSRPHRAHPLFAGLVDAGVVMLEP"
  },
  {
    "reference": "MSKLTDLTMAEARDGLAKGAFTAVELTDAHIKATEAQRHLNAFIVETPDLALEAAKASDARRQAGTAGSMDGLPIGIKDLFCTEGVQTTAASHILEGFKPPYESTVSGKLKAAGAISLGKLNLDEFAMGSSNQTSYFGAVENPWKKTSDPKAKLVPGGSSGGSAAAVAARMVLGATGTDTGGSIRQPAAFCGITGIKPTYGRCSRFGIVAFASSLDQAGPMARTVRDCAIMLGAMAGHDPKDSTSVNMAVPDFERALTGDIRGLKVGIPKEYRPDGLSDEVAKVWDRGIEWLKAAGATPVEISLPHTKYALATYYIIAPAECSSNLARYDGLRYGLRVPGKTLDDMYKKSRAAGFGAEVRRRILIGTYVLSAGYYDAYYAKAQKVRRLIAEDFRKAFETVDVILTPTAPSAAFGMGEGTDDPVTMWLNDVFTIPTSMAGLPGLSLPAGLSADGLPLGLQLIGRPFDEETVFRVAGVMETAANFTAKPEGV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must be optimized for glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity. The designed protein sequence is ",
    "response#1": "KRTINEGGIVAALKAPGLRGILGFELQPGRRFGSELADRVRAWTRLGGLMHSDELPGYGISKEEVAAVASRLGL",
    "response#2": "KRTINEGGIVAALKAPGLRGILGFELQPGRRFGSELADRVRAWTRLGGLMHSDELPGYGISKEEVAAVASRLGL",
    "response#3": "DLFEETAKHYSNYKKLVNLLINDYLRWLNDKNLRPSQSKAGSQHLVELLKLLDDGVITIKIAKEILPEIVLEGKMPSSIIKERGLVAIKDEDYLINVIKEVINEEPDAAEKAKNDPKVINYLVGKVMKKTGKRADPQLTNELIKKIL"
  },
  {
    "reference": "MDIPSSNQIQHGQRSERNRRMPRASFSSTATTSTAATLTSAMVLDQNNSEPYAGATFEAVPSSIVSFHHPHSFQSSNLPSPHSSGNLEQRGRRLTESEPLVLSSAEQSRSSSRNPSHFRFFTQEQISNAEGASTLENTDYDMAWDATPAYEQDRIYGTGLSSRRSSIRSFSRASSLSNAKSYGSFSKRGRSGSRAPQRLGENSDTGFVYHSATHSSSSLSRYTTRERIPIELESQTDEILEDESSTHSLESSDSRRSASENNRGSFSGHDDVHNQHSEYLKPDYHEKFYPQYAPNLHYQRFYIAEEDLVIGIAAYQTSKFWYIIYNLCCFLTFGLVYLLTRWLPHLKVKLYGVKVPLAKAEWVVIENEFGEFVIQPIDRQWYNRPLSTVLPFENYPNPSYEPNDINLSHHHANEINPNVPILITFEYRYIKFIYSPLDDLFKTNNNWIDPDWVDLSTVSNGLTKGVQEDRELAFGKNQINLRMKTTSEILFNEVLHPFYVFQVFSIILWGIDEYYYYAACIFLISVLSIFDSLNEQKKVSRNLAEMSHFHCDVRVLRDKFWTTISSSELVPGDIYEVSDPNITILPCDSILLSSDCIVNESMLTGESVPVSKFPATEETMYQLCDDFQSTQISSFVSKSFLYNGTNIIRARIAPGQTAALAMVVRTGFSTTKGSLVRSMVFPKPTGFKFYRDSFKYIGFMSLIAIFGFCVSCVQFIKLGLDKKTMILRALDIITIVVPPALPATLTIGTNFALSRLKEKGIFCISPTR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should contain Cytoplasmic, Vacuolar topological domains.2. For general function, the protein need meet that Vacuolar transporter which plays a role in sequestration of divalent heavy metal ions.3. Target a Polar residues, Basic and acidic residues compositional bias in the protein's composition for improved properties.4. We are looking for a protein with broad substrate specificity for ATP + H2O = ADP + H(+) + phosphate.5. The designed protein should be involved in transmembrane transport, cellular calcium ion homeostasis by localizing to fungal-type vacuole membrane and exhibiting manganese ion binding, P-type ion transporter activity. The designed protein sequence is ",
    "response#1": "FLHITDIHPDPYFHVGAVAEDKCHVDPDHKDSDYPDEDSELVWFRATGKKKHNHHKGKDHEKMPKAGYYGHPLSSCDGPISLMNATFDWIDQNIRDEIDFIIWTGDNVRHDNDNRYPRLEQDIFGYNQIVSSKFHELFRYNETRDGEGHNGGGDPQHPLVIPIIPSLGNNDVFPHNLYLAGPSFQSRRMLQIWSEFVPEAQQHIFSRGSYYFQEVITGKLAVISLNTLYFYKSNPMSDGCDEKTDPGYKHLVWLGVVLDEMRQRGMKVWLSGHVPPVEKNYEDSCHLKLAYWLTEYRDIIVGSVFGHMNIDHFVVMDPKKIAIGDDMTRGDHSEVSNQMYANYAIGKDVQAMKAVVGEEALSSEDKLYLEFLERFESSFVGQNHYENRDIFNSLDLGWSLLRTFPSNLLKRITEKTIKQYK",
    "response#2": "KEQELLHQAWVDLQHIAKYEHAYGSSGNDYVHDYLESRIVSAVAHKSYVEYDNDLNYTNNIMFGSRSELSGNSFNSVSYYESNNLVVRINGTDETLPALLLSAHFDSVPSSFGVTDDGMGIASLLGVLYYYTGKSTARPRRTIVLNFNNDEEFGLYGATSFLSHPWATGVHYFLNLEGTGAGGKAILFRGTDYGITKYFKGVRYPYGTSIFQQGFNNHLIHSETDYKIYKEKGGLRGLDVAFYKPRDLYHTAGDNIKNIDIKSLWHMLSNALDFTAIVTKGKIDLDADSLDSESSKSNTDTAVYTSFLNFFFAFN",
    "response#3": "HEQAVLSRSWESLQKIARTKHTYTSEGNDEVHAYLEAHIASLVAKKPYMELDTDKNGTRRVMFDVKYLSYDSVSYYESNNLVVRVNGSDSSLPALLVSAHYDSVPTSYGVTDDGMGVASMLGLLEHYSSVAQPKRTIIFNFNNNEEFGLYGAQAFLAHPWFSQIAYFLNLEGTGAGGKAILFRGTDYGIVRHFSSVRFPFASSLFQQGFNNRLIHSETDYSVYIKAGLRGLDLAFYKPRDIYHTTRDSIQNTNIKSLWHMLSSSLDFVEHVSSQTIDLDEEVHAEAGKRDLALYTSFWR"
  },
  {
    "reference": "MNVNELRSRAASQEQDPCTLSNYRGFEVVQSSLDLNVSFEKKNVSGTVTYELKNLKSTTEVVLDTSFLDIEQAFVDEKAVDFKLEARKEPFGSPLVIKLANVVEKLKISIKFATTENCSALQFIDKEATDSKVCDYLFSQCQAIHARSLFPCFDTPAVKSPYSFHVKSPSYTLMSGRPVECSEENTYRFEQPIPIPSYLVAIASGNLAGAPIGPRSTVYTEPPNLKACQWEFEKDMENFLVVAEDLIYKYEWLKYDALILPSSFPYGGMENPNITFATPTLISKDRSQVKVMAHELAHSWSGNLVTNCTWEHFWLNEGWTVYLERRILGGVASYEAKQRGEKDYVDAGEKVRHFAAILGWNDLVDTVKTIPSQYTRLVWDLKTVTPDDAFSKIPYEKGFSFLFYLETVLGGTDEFDPFMKHYFKKYRYKSLDSYQFIDTLYEFFEPKGKKDVLDSVDWNTWLYGEGVPPFTPKYDTRLADECYHLRDKWAAYEQNKGQFSASDIEHFEVNQHLLFLGTLTELFSNKKPAPEVYEELRKVYHQYSEASNCEIIASWNDLLLKSENFKPSDKIVQNFATWLGTVGRMKFARPGYKLLKDYVDKDLAIATFRKFESRYHPICKAMVRKDLGLDS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The desired protein should be able to perform an epoxide + H2O = an ethanediol The designed protein sequence is ",
    "response#1": "MFYINGKKITEKNVDPEEMLLTYLRKKVRLTGTKYACGTGGCGACTVMISRYNPFQEQTMRASHFSVNACLVPICSLHGAAVTTAEGIGSTKTKLHPVQERIAKAHGSQCGFCTPGMVMSMYTLLRNIPEPTMEDIREALTGNLCRCTGYRPIIDGFKTFCGASGCCQDEESTTGCCLDQGPNLNQMDSDVSNELFRMNDVLPLDPTQDLIFPPELMLMAKKQGAQPLCFQGNRIRWISPVDLADLLELKSLYPDAPLVIGNTTVGLNVKLKGVFHPLIIAPGRIAELNMIKEGKYGLTFGAACSLSAMKDELKRAVSELAEEKTKVFQALLQTLSCLAGKQIRNMATLGGNILSAIPKYDLNSILAAADCTLLVVSKGGSREIRLNEEFFTGFGKTTLKPDEVLLTVHIPYSRKWEFVSAFRQAQRREFAFSIVNSGMRVVFEEGTNVVKSLNIFYGGVGPTLIRASYTCQELAGRLWDEKTLQEACRLLGEEISLSPSAPGGRVEFKKTLTLSFFFKFYMQ",
    "response#2": "MFYINGKKITEKNVDPEEMLLTYLRKKVRLTGTKYACGTGGCGACTVMISRYNPFQEQTMRASHFSVNACLVPICSLHGAAVTTAEGIGSTKTKLHPVQERIAKAHGSQCGFCTPGMVMSMYTLLRNIPEPTMEDIREALTGNLCRCTGYRPIIDGFKTFCGASGCCQDEESTTGCCLDQGPNLNQMDSDVSNELFRMNDVLPLDPTQDLIFPPELMLMAKKQGAQPLCFQGNRIRWISPVDLADLLELKSLYPDAPLVIGNTTVGLNVKLKGVFHPLIIAPGRIAELNMIKEGKYGLTFGAACSLSAMKDELKRAVSELAEEKTKVFQALLQTLSCLAGKQIRNMATLGGNILSAIPKYDLNSILAAADCTLLVVSKGGSREIRLNEEFFTGFGKTTLKPDEVLLTVHIPYSRKWEFVSAFRQAQRREFAFSIVNSGMRVVFEEGTNVVKSLNIFYGGVGPTLIRASYTCQELAGRLWDEKTLQEACRLLGEEISLSPSAPGGRVEFKKTLTLSFFFKFYMN",
    "response#3": "MFYINGKKITEKNVDPEEMLLTYLRKKVRLTGTKYACGTGGCGACTVMISRYNPFQEQTMRASHFSVNACLVPICSLHGAAVTTAEGIGSTKTKLHPVQERIAKAHGSQCGFCTPGMVMSMYTLLRNIPEPTMEDIREALTGNLCRCTGYRPIIDGFKTFCGASGCCQDEESTTGCCLDQGPNLNQMDSDVSNELFRMNDVLPLDPTQDLIFPPELMLMAKKQGAQPLCFQGNRIRWISPVDLADLLELKSLYPDAPLVIGNTTVGLNVKLKGVFHPLIIAPGRIAELNMIKEGKYGLTFGAACSLSAMKDELKRAVSELAEEKTKVFQALLQTLSCLAGKQIRNMATLGGNILSAIPKYDLNSILAAADCTLLVVSKGGSREIRLNEEFFTGFGKTTLKPDEVLLTVHIPYSRKWEFVSAFRQAQRREFAFSIVNSGMRVVFEEGTNVVKSLNIFYGGVGPTLIRASYTCQELAGRLWDEKTLQEACRLLGEEISLSPSAPGGRVEFKKTLTLSFFFKFYMKR"
  },
  {
    "reference": "MANPKYIFCTGGVVSSVGKGVTAAAIGRILKARGLKVAIQKLDPYLNVDPGTMSPYQHGEVYVTADGAETDLDLGHYERFIDESLTRASNVTAGQVYSHVIDKERRGDYLGGTIQVIPHITNEIKRRVTLVGQENNADVVIVEVGGTVGDIEGQPFLEAIRQMRLSAGRNNSLYIHVTFLPYIGATQELKTKPTQHSVRELRSLGIQPQVIIARTDHDVSKEVIDKIALFCDVEQRAVIPLKTADLIYNVPLMLEDAGLGDYLVEQLQLAVQSPDLSEWRSMIQRMRAADKPVKIAVVGKYVELHDAYMSMKEALFHAATAADRDVEIVWINSGDLERDRGWDALKSAHGIVVPGGFGYRGVEGKILAGRFARERQVPYLGLCLGMQTMCIEFARNVIGLEDANSAEFEATSDHFVIDLMLEQRGVTNLGGTMRLGVYPCELTVGSVAAQAYTNAPLTHERHRHRFEFNNRYRRQFEQHGMRFSGLSPDGELVEIAEIAGHPFMVGSQFHPEFLSRPTMPHPLFSAFMRAAIAHEQQSNASSPATDGAQPPHNHAQPTNSVGD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Ideal protein should have a binding affinity for key enzymes in the CTP from UDP: step 2/2 pathway to enhance the overall efficiency of the pathway.2. The designed protein must have a Nucleophile that is structurally stable and resistant to denaturation.3. The Mg(2+), UTP, CTP binding site should be stable and able to withstand changes in environmental conditions.4. The protein must have high specificity towards metal ion binding, CTP synthase activity.5. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.. The designed protein sequence is ",
    "response#1": "LSDAYLSVVESLVHGGIAVNSEVKLNWVNAEDIEQYGAEKFLKDVSGILVPGGFGIRGVDGKVQAIQYARQQKIPFLGLCLGMQCAVIEWARNIARLERSNSAEFDPETPNPVINLLPEQEDVVDLGGTMRLGLYACRLSPDTLASSLYPQEVIYERHRHRYEFNNAYRSLLIETGYVVSGTSPDGRLVEIIELPGHPFFIATQFHPEFQSRPNSPHPLFLGFVEYAEAIGRF",
    "response#2": "LPDAYMSVTEALHHGGIEHDAQVKITWINAEELEEPNADLDEIFVGCKGILVPGGFGDRGVEGKIRAIQYAREHEIPFLGLCLGMQCAVIEFARHVAGLTNAHSTEFVPETPHPVIALMEDQQDVEEKGGTMRLGAYPCLLADGSHSRAEYGAGEISERHRHRFEFNNAYRAQLEEKGMVIAGTSPDDRLVEVVEIADHPWFVASQFHPELKSRPNHPHPLFAGFVES",
    "response#3": "EDSYVSVIEAIKHASGNKKVVPEIVWMDSKDFEKDENKLQKLNDIDGVIVPGGFGSTGIEGKILVAKYCRENNVPYLGLCLGLQIAVIEFARNVCGLEGSNSTEFDKNTKHPSVCILPDQVEKMKKSQYGGTMRLGGQDVKIVENTIAEKIYGKNLIRERFRHRFEINPELIENLKNKGLVFSGMTPDNKIMQIIELSEHIFFMGSQFHPEFTSRPINPNPLFTKFILSDAYLSVVESLVHGGIAVNSEVKLNWVNAEDIEQYGAEKFLKDVSGILVPGGFGIRGVDGKVQAIQYARQQKIPFLGLCLGMQCAVIEWARNIARLERSNSAEFDPETPNPVINLLPEQEDVVDLGGTMRLGLYACRLSPDTLASSLYPQEVIYERHRHRYEFNNAYRSLLIETGYVVSGTSPDGRLVEIIELPGHPFFIATQFHPEFQSRPNSPHPLFLGFVEYALN"
  },
  {
    "reference": "MTWTSGQLQREKQARPGSGAVLAFPDDKDLRVYGPAESQSAVFGDVCPLLTSLLDGYNVCVMAYGQTGSGKSYTMLGRHSDDGPVLPLDPQSDLGIIPRVAEELFRLILENTSRSPKVEVSIVEVYNNDIFDLLAKDSIAAVSGVKREVVTAKDGRTEVALLASEAVGSASKLMELVHGGLQLRAKHPTLVHADSSRSHLIITVTLTTASCSDSTADQACSATLPREQTEAGRAGRSRRASQGALAPQLVPGNPAGHAEQVQARLQLVDSAGSECVGVSGVTGLALREMACISRSLAALAGVLGALLEHRGHAPYRNSRLTHLLQDCLGGDAKLLVILCISPSQRHLAQTLQGLGFGIRARQVQRGPARKKPPSSQTEGKRRPD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that May also act as a negative regulator of amino acid starvation-induced autophagy.2. The designed protein must be able to perform minus-end-directed microtubule motor activity under specific conditions.3. The ATP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "DIADLEDAKKLLREAVVLPMWMPDFFKGIRRPWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPTTIFIDEIDSICGRRGTSDEHEASRRVKSELLIQMDGVGGALEGDDPSKLVMVLAATNFPWDIDEALRRRLEKRIYI",
    "response#2": "PWKGVLMVGPPGTGKTLLAKAVATECKTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPTTIFIDEIDSMCSRRGTSEEHEASRRVKAELLVQMDGVGASENDDPSKMVMVLAATNFPWDIDEALRRRLEKRIYIPLPSREELINRLLVK",
    "response#3": "VCMFGPPGTGKTMLAKAVATECNTTFFNVSASTLTSKYRGDSEKLVRLLFEMARFYAPSTIFIDEIDSICSRRGSESEHEASRRVKSELLIQMDAKVLLGVISSNPNSAAGVLVLAATNFPWDLDEALRRRLEKRVFIPL"
  },
  {
    "reference": "MAKYSIIMEKKNKLFDRTEYIIQVEHPKEKTPTREEAKEKIAEMLNVDKNKLVIKKIVSKYGLPYSFIYARVYDNIDTAKRVELKQILRRNNLQ",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have structural constituent of ribosome within ribosome, ribonucleoprotein complex to regulate translation. The designed protein sequence is ",
    "response#1": "IGNITKIKNVPEGMAVNSVESVYGDGGTFAMVNGSYSLVVNHRKETNETVIKIPSGKKLTVSSECRCIVGVVAGGGIHDKPLLKASVAHYKAKARGHVFPRVRGVAMNPVEHIHGGGNHQHVGKPTTVG",
    "response#2": "IGNITKIKNVPEGMAVNSVESVYGDGGTFAMVNGSYSLVVNHRKETNETVIKIPSGKKLTVSSECRCIVGVVAGGGIHDKPLLKASVAHYKAKARGHVFPRVRGVAMNPVEHIHGGGNHQHVGKPTTVG",
    "response#3": "IGNITKIKNVPEGMAVNSVESVYGDGGTFAMVNGSYSLVVNHRKETNETVIKIPSGKKLTVSSECRCIVGVVAGGGIHDKPLLKASVAHYKAKARGHVFPRVRGVAMNPVEHIHGGGNHQHVGKPTTVG"
  },
  {
    "reference": "MARRHGWQLPAHTFQVVAITVFFLLTVAYYAFFAPFLGNKLYEYIAIGVYSFLAFSVLVLYIRCTGIDPADPGIFVKADNTPAHKSQNSNYVPENASAIDGGPYIRHGSGCCSAIGRFICGCLVIQDCRRDTQQEQSNEQEEALFCSLCNAEVRMFSKHCRSCGKCVDGFDHHCRWLNNCVGQKNYISFVCLMAASFFWLIAEFGVGVTVFVRCFVDQKAMEHLITEKLGLGFSRPPFAAVVVVCTTLSLLALIPLGELFFFHMILIRKGITTYEYVVALRAQTEPLGTSVDELDQTSQYPSPASSAVTATSARSSLGLSIQYRGASLCTPPNIFVDQQDDVIQHLEPGPVRSTIDPDSLSQKKPPQRQQVRINPWKLAKLDSKEASKAAAKARASSSVLLPVSSRQNPYKTSSNVSGRSSPGRGKPADSESCSLSSPGLTRDHFNPMYMSSPANESPLNEEESRNAVVAARRNLPSSDESSVVWDPEAGRFVSLSRIPGTDVGGPLGNECLNTITSTGTDRSRRARGNPLTGYFQQVRSQRGDQLPVFMPTDSQLHRHLSTRFH",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.2. The designed protein must have a S-palmitoyl cysteine intermediate active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "IVITGVSGRFPRSENVAEFGEHLFAGEDLITEDDLRWPPGYYDLPKRHGKLKDLKKFDAQFFGVTPKQANYMDPQVRLLLEVAYEAMIDAGINPQDLRGTKTGVFVGCSASETGGALTQDPETVTGYTLTGCVRSMFSNRLSFAFDLRGPSFSVDTACSSSMCALQLAIDAMRQDQCDAAIVAGAHLTLTPTAALQFLRLGMLSDKGSCRSFDESGDGYCRTEGVAAILLQRAPNAKRVYATVVHAKSNTDGYKEQGITFPSGERQAALLEEVYREANVDPNTVTYVETHGTGTKVGDPQEANAICNVFCSNRTPDNPLLIGSVKSNMGHAEPASGLCSIAKVLLAMERNEIPPNLHFKEPNPYIPGLSDGRLKVVSEKTPFKGGYVGINSFGFGGSNTHVLLKAADDDSKTNLIGLSRDQLIAEMASIGEKPFRAKQLWHWMYNRGETDFAKMTSISKSMHGALAERYVVRRPGVTKELISADTTRKWLLKFDDGHEAETVYIPDADEERGAVCISTQVGCTLTCRFCHTGTQLLVRNLSAAEIVGQFMVARDSYGEWPTPDDGGRQLSNIVVMGMGEPLYNFENVATALEIAMDGEGIGISKRRITLSTSGVVPMMKECGERLGVNLAVSLHAVTDEIRDRIMPINKKYPLKELMQACREYPGASNARRITFEYIMLKGINDSAADARALLKLVKGLPAKFNLIPFNPWPGSEFDTPDIKTTKAFSDILQDAGYSAPIRMPRGRDILAACGQLRSESQRERAS",
    "response#2": "VYSGMGSQWSGMAQELMSIPLIDESLRASSKSLESYGVDVYKMLKTTDATQYQNNTLNCMLAISAIQIAITDLLIFLGVVPDGIIGHSTGEMGCGYADGALTREQTMKLAYHRGKSIMTSKLPIKGGMAAVGLTWEEAFKRCPEGIVPACHNGADSVTISGEETKVKQFVEELTEEKIFAKFVDSSGIPFHSPIMLSVKDSMLSSMKTAIIDPKPRSSRWISTSIL",
    "response#3": "VYSGMGSQWSGMAQELMSIPLIDESLRASSKSLESYGVDVYKMLKTTDATQYQNNTLNCMLAISAIQIAITDLLIFLGVVPDGIIGHSTGEMGCGYADGALTREQTMKLAYHRGKSIMTSKLPIKGGMAAVGLTWEEAFKRCPEGIVPACHNGADSVTISGEETKVKQFVEELTEEKIFAKFVDSSGIPFHSPIMLSVKDSMLSSMKTAIIDPKPRSSRWISTSIR"
  },
  {
    "reference": "MFDITLVHAREILDSRGNPTVEAEVTLSGGAVGRAAVPSGASTGEHEAVELRDGDNARYLGKGVLKAVENVEGEIAQALGGMDASQQRDIDMRMLDLDGTENKGRLGANAILAVSMASARAVANQLDIPLYRYLGGVNGNILPVPMMNILNGGAHADNNVDFQEFMAMPVGAESFSEALRWGAEVFHTLKGVLKKRGYNTAVGDEGGFAPSLKSNVEAIEVILEAIQQAGYTPGEQIAIALDPAASEFFKDGKYIFKKSDKSEKTSEQMVRWWSDWANKYPIVSIEDGLAEDDWEGWKLLTDELGDKIQLVGDDLFVTNPERLARGIDNGVANSILIKVNQIGTLSETLDAIEMARRNGYTAVISHRSGETEDTFIADLAVATGAGQIKTGSASRTDRVAKYNQLLRIEEQLGAGARFLGIGALNYDGDLSAE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that can perform magnesium ion binding in extracellular region, cell surface, phosphopyruvate hydratase complex to support glycolytic process.2. A protein that is capable of catalyzing reactions within the Carbohydrate degradation, pyruvate from D- glyceraldehyde 3-phosphate: step 4/5 pathway with high efficiency and specificity.3. The designed protein should have a high specificity for substrate, Mg(2+) binding, with minimal off-target effects.4. The protein with the reaction (2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate, the Proton donor, Proton acceptor must be optimized to ensure efficient catalysis.5. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.6. For general function, the protein need meet that Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate; It is essential for the degradation of carbohydrates via glycolysis. The designed protein sequence is ",
    "response#1": "MPLPLVNVINGGLHADNLLDFQEFMIVPVGAKTFADAAQICAEVFYKLKEVLKKMGHSTNTGDEGGFAPNLENNTDVLDVLVEAIEKSGYRASSDVALAMDVAASTFYDGTSYKFSGKCLTSGELIACYEDMVSRYPIISIEDAMAESDIEGWKAVTKRLGDKIQLVGDDLFVTNPALIANGVENKLANAVLVKPNQIGTLTETIDAIRSAQRNNYNVIISHRSGETEDVTIAHIAVAANCGQIKSGSFSRSERLAKYNELLRIEEDLGKSRGILVKLNQIGTVSELTGKLLPVPLMNIINGGEHAPNNLDIQEFMIMPIGAETFAEALRMGAEIFHTLKGLLAADGHNTAVGDEGGFAPNLESHAQAFEYIMKAIEAAGYRPGADVALAIDAAASEFYKDGKYVLAGEGKEFDAQGMIDFYTDFVERFPLISIEDGLAEGDWEGWKKMTDDLGEKIQLVGDDLFVTNPEILAEGIEQGACNSILIKLNQIGTLTETLDTMELAKTAGYTNVVSHRSGETSDHFIADLAVGLNAGQIKTGSLCRSDRLAKYNQLLRIEEDLEDDGIYYGPALKEV",
    "response#2": "MPLPLVNVINGGLHADNLLDFQEFMIVPVGAKTFADAAQICAEVFYKLKEVLKKMGHSTNTGDEGGFAPNLENNTDVLDVLVEAIEKSGYRASSDVALAMDVAASTFYDGTSYKFSGKCLTSGELIACYEDMVSRYPIISIEDAMAESDIEGWKAVTKRLGDKIQLVGDDLFVTNPALIANGVENKLANAVLVKPNQIGTLTETIDAIRSAQRNNYNVIISHRSGETEDVTIAHIAVAANCGQIKSGSFSRSERLAKYNELLRIEEDLGKSILIKLNQIGTLTEG",
    "response#3": "MPLPLVNVINGGLHADNLLDFQEFMIVPVGAKTFADAAQICAEVFYKLKEVLKKMGHSTNTGDEGGFAPNLENNTDVLDVLVEAIEKSGYRASSDVALAMDVAASTFYDGTSYKFSGKCLTSGELIACYEDMVSRYPIISIEDAMAESDIEGWKAVTKRLGDKIQLVGDDLFVTNPALIANGVENKLANAVLVKPNQIGTLTETIDAIRSAQRNNYNVIISHRSGETEDVTIAHIAVAANCGQIKSGSFSRSERLAKYNELLRIEEDLGKSLVK"
  },
  {
    "reference": "MERYVTIASCQLNQWAMDFEGNRLRIIDSIKEAKRQNASLRVGPELEVTGYGCEDHFLESDTYYHSWEMLCSIIHDPDCQDILLDIGMPVMHKAMRHNCRILALNGKILLIRPKIWLCDDGNFRESRWFTPWLRPRVVETHYLPTFVAKSLNQTTVPIGDAILQCNETVVGVETCEELFTPNSPHIDMALDGVEIFINASGSHHELRKLTTRVNLIQNATEKCGGIYLYSNQRGCDGGRLYYDGSSMIFANGKMLAQGHQFSLKDVEVISATVDVDTVRSYRFQPSHGIQGVTRPSYERIHVNFSLSSYQQDYDIYRKPTDPIEVTIPLPEEEITFGPACWLWDYLRRSHAAGFFLPLSGGLDSCSTAVLVYSMCRIVCKAMEEDDAQVLSDVRRIVGDPSYSSTDPKKLLNHLFYTAFMGSEHSSKETRSRAKELSSLIGSYHTDVNIDTMTSAVVKLFALVTGKTPQFRSNGGTNAENLALQNIQARSRMLLGYLFAQLLPWVRGYSGSLLVLGSSNVDECLRGYLTKYDCSSADINPIGGISKTDLKSFLRYAKEALDLPILQEFLDATPTAELEPTTESYVQSDEADMGMTYAELSVFGRLRKISKCGPYSMFTQLMHQWGDRLSPSQVAEKVKRFFHYYGINRHKMTTLTPSYHAETYGVDDNRYDLRQFLYPSWTWQNKKIDALASKFEQHQRK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that can modulate the overall flux of metabolites through the NAD(+) biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "FSFADFGTRRRASKAWHEEVLLTLKEQLPASFSGTSSLHFARTLGLPPIGTMAHEWFQSWQAVTRLADAQKAALEGWVQEYRGRLGIALTDCYSMDSFIRDFSDPYFGKLYDGLRHDSGCPFVWAEKAILMYQQMGIDPLSKTLVFSDGLNFPRMLEIWKRFKGRAKISFGIGTNLTNDVGNVPLNIVIKMIAANGRPIAKISDEPGKSMCEDLGYLQYLASQYL",
    "response#2": "FSFADFGTRRRASKAWHEEVLLTLKEQLPASFSGTSSLHFARTLGLPPIGTMAHEWFQSWQAVTRLADAQKAALEGWVQEYRGRLGIALTDCYSMDSFIRDFSDPYFGKLYDGLRHDSGCPFVWAEKAILMYQQMGIDPLSKTLVFSDGLNFPRMLEIWKRFKGRAKISFGIGTNLTNDVGNVPLNIVIKMIAANGRPIAKISDEPGKSMCEDLGYLQYLASQYY",
    "response#3": "PLCLGIDPHPELLRAWGLGTDAAGLAGFSDICVAAFAGFAVVKPQVAFFEAYGSAGYAVLERTIAALRESGVLVLADAKRGDIGSTMAAYASAWAGDSPLAADAVTASPYLGFGSLQPLLDTAREHDRGVFVLAATSNPEGASVQCAVAPGAIPGRTVAQAIVDAAAEVNAGAARGSVGVVVGATLSAPPDVSALNGPVLVPGVGAQGGRPEALAGLGGARRGQLLPAVSREVLRAGPDVAALRR"
  },
  {
    "reference": "MANPPHGGILKDLIARDAPRRQQLFDEAEGLPAIVLTERQLCDLELILNGGFSPLEGFLNQKDYEGVVNNLRLADGNLFSIPITLDLSKESIEELGVKPGARVALRDFRDDRNLAIITVEDVYQPDKKKEAQEVFGGDPEHPAVKYLFDTAQEFYIGGKVEAIDRLNHYDYVALRYTPAELRLHFDKLGWSRVVAFQTRNPMHRAHRELTVRAARARQANVLIHPVVGLTKPGDIDHFTRVRVYQALLPRYPNGMAVLGLLPLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGKNSKGVDFYGPYDAQYAVEKYRDELGIEVVPFQMMTYLPDSDEYKPKDEVAEGVRTLDISGTELRKRLRTGQEIPEWFSYPEVVRVLRESHPPRNKQGFTVFLTGYQNSGKDAIARALNVTLNQQGGRSVSLLLGETIRHELSAELGFSREDRDRNIARIAFVASELTKSGAAVIAAPIAPFEEARQHAKETVEKNGSFFLVHVATPLEFAEKTDKRGIYAKARNGEIKGFTGVDDPYEAPNNAHLSVDLSKTNVRTAVHQIVLLLEAQGLLTQL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein contains novel ATP-sulfurylase PUA-like domains that confer a unique function or activity.2. The designed protein should have a high specificity for sulfate binding, with minimal off-target effects.3. A protein that can modulate the overall flux of metabolites through the Sulfur metabolism pathway, enhancing or suppressing its activity as needed.4. The protein must exhibit the following characteristics: Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids..5. The active site of the protein must be engineered to enable effective catalysis of ATP + H(+) + sulfate = adenosine 5'-phosphosulfate +   diphosphate.6. The designed protein should have adenylylsulfate kinase activity, ATP binding to facilitate hydrogen sulfide biosynthetic process, methionine biosynthetic process, sulfate assimilation. The designed protein sequence is ",
    "response#1": "MNLPPHGGVLKDLLARDASIAASLLEEAETLPDIVLNERQLCDLELIITGGFSPLEGFMNKADYEGCLTNMRLADGSLFPMPITLDVSKEQVQSLGLEEGRRVTLRDPRDDAVIAILTVSDLFDVDRSREAKLVMGADDQAHPAVAFLYNNVKDVYVGGAVQAINTPHGGVLKDLFARDLPRHAELRDESEKLPALVLTERHLCDLELILNGGFSPLEGFMIEKDYNGVVDNNRLADGALFSMPITLDVDQGQLDHLGIKAGARITLRDSRDDRNLAILAVEDVYRPDNTSNRVKEAKEVFGSDDDTHPGVKHLFNTAKEFYVGGKLEAINDLRYTPAEIRLHFDKLGWTKVVAFQTRNPMHRAHRELTVRAARSHHANVLIHPVVGLTKPGDIDHFTRVRVYKALLPRYPNGMAVLGLLPLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGKNSKGVDFYGPYDAQYAVEKYRDELGIEVVPFQMMTYLPDSDEYAPIDTIPKDVRTANISGTELRSRLRSGRDIPEWFSYPEVVKVLRPPHGGVLKDLVARDEPLHQNLKAEATTLPDLVLTERQLCDLELITNGGFSPLEGFMNEADYKSVVDTLRLADGVLFPMPITLDVSQEDVTRLSLAPGARVALRDPRDDEALAIITIEDIYQPDRVKEAIQVFGADDPAHPAVAYLRNKVKDYYIGGKLQAIAPHGGILKDLVARDEPISAQLGEEASRLSDIILTERQLCDLELIINGGFSPLEGFMNEQDYNSVVDTLRLADGTLFPMPITLDASRDDVERLSITAGARITLRDPRDDQALAIITVDDVYTHDRVREAIQVFGADDPAHPSVSYLRNRVKEFYIGGKVQAI",
    "response#2": "QPHGGELLDLVQRDASKRDQLLAEAKSLPQLVLTPRQLCDLELLLNGGFSPLDGFLNQDDYESVVSEMRLASKINPATGKGYLWPIPITLDVDAASAQSLKQGERVVLVDLRDETPLAILTVESVYQPNKQVEGEKVFRGDPEHPAVKYLHETAGDWYVGGKLQGLFRADDKSYVDLRYTPAELRTHFDKLGWTRVVAFQTRNPMHRAHRELTVRAARARQANVLIHPVVGLTKPGDIDHFTRVRVYQAILPRYPNGMAVLGLLPLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGKNSKGVEFYGPYDAQHAVEKYKDELGIEVVEFQQVTYLPDTDEYKPVDEVPPGTKTLDISGTELRRRLRTGGHIPEWFSYPEVVKVLREQHPQWDQAAIDAPHGGILQDLIARDASKKDALLKEVQKGKLTQWSLTARQICDIELILNGGFSPLTGFLNEKDYLGVVNNSRLADGTLWTMPITLDVDEKFASSVSSNSRILLLQDNEIPIAILTVADVYKPDKQLEAKNVFRGDPEHPAIRYLNNIAGDYYIGGSLEAIYRSDDKSYVADLRYTPAELRTHFDKLGWTRVVAFQTRNPMHRAHRELTVRAARARQANVLIHPVVGLTKPGDIDHFTRVRVYQAILPRYPNGMAVLGLLPLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGKNSKGVEFYGPYDAQHAVEKYKDELGIEVVEFQQVTYLPDTDEYKPVDEVPPGTKTLDISGTELRRRLRTGGHIPEWFSYPEVVKVLR",
    "response#3": "AAHGGILKDLVARDDAISSHLREEAFTLPDIVLTERQLCDLELIINGGFSPLEGFMNEKDYQSVVDTLRLADGTLFPIPITLDISKEDIDRLSLAPGVRVALRDPRDDQALAIITVDDIYTPDRVKEAIQVFGADDPAHPSVAYLRNRVQDFYIGGKVQAITPHGGQLNDLIIRDAHIKQNLLQESATLPSITLTARQLCDLELILNGGFSPLAGFLNEDDYTSVVNDLRLSSVKGANGKGLLWPIPITLDVNEQTASSYANGSRIVLRDLRDETPLAILTVESIYKPNKAVEAEKVFRGDPEHPAVKYLIETAGDYYIGGSLQGIELRYSPAELRSHFDKLGWSRVVAFQTRNPMHRAHRELTVRAARSRQANVLIHPVVGLTKPGDIDHFTRVRVYKALMTRYPGGMAALCLLPLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGKNSKGVDFYGPYDAQDAVNKYRDELGIEVVPFQQMTYLPDSDEYKPKDEVEAGIRTLDISGTELRRRLRTGAPIPEWFSYPEVVRVLRPPHGGVLKDLLARDASIAASLLEEAETLPDIVLNERQLCDLELIITGGFSPLEGFMNKADYEGCLTNMRLADGSLFPMPITLDVSKEQVQSLGLEEGRRVTLRDPRDDAVIAILTVSDLFDVDRSREAKLVMGADDQAHPAVAFLYNNVKDVYVGGAVQAINDLRYTPAELRTHFDKLGWTRVVAFQTRNPMHRAHRELTVRAARARQANVLIHPVVGLTKPGDIDHFTRVRVYQAILPRYPNGMAVLGLLPLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGKNSKGVEFYGPYDAQHAVEKYKDELGIEVVEFQQVTYLPDTDEYKPVDEVPPGTKTLDISGTELRRRLRTGGHIPEWFSYPEVVKVLR"
  },
  {
    "reference": "MLRTEWIARRHGHANVSQMYYARQGLITEEVAYVAHRENLEPELVRSEVARGRLIIPANTNHPNLEPMGIGIATRCKVNANIGASPNASNLGEEVAKLELAVKYGADTVMDLSTGGGDLDAIRSAIIQASPVPIGTVPMYQALEAVHGNVEKLTPEHILDVIEKHAKQGVDYMTIHAGILIEHLPLTQGRLTGIVSRGGGILARWMLAHHKQNPLYTHFDEIINIFKKYDVSFSLGDSLRPGCQHDASDAAQFAELKTLGELTRRAWTHDVQVMVEGPGHVPMHQIEMNVRKQMQECSEAPFYVLGPLVTDIAPGYDHITSAIGAALAGWYGTAMLCYVTPKEHLGLPNAEDVRTGLIAYKIAAHAADIARGRPGARDRDDELSRARYNFDWNRQFELSLDPERAREYHDETLPADIYKSAEFCSMCGPKFCPMQTKIDSQALSELEAALKPQPVAAQE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects.2. For general function, the protein need meet that Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.3. The designed protein should be optimized for carbon-carbon lyase activity, zinc ion binding activity in order to improve thiamine biosynthetic process.4. A protein that is capable of catalyzing reactions within the thiamine diphosphate biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MSPYPNSQKSYLLGSRPDIRVPVREITLSDTLVGGSKDAPIFEPNEPICVYDTSGVYTDPSHDIDLYKGLPKLREEWIEERRDR",
    "response#2": "MSEPLPKSRKVYETGSRPDIRVPFREIEQADTPTMFGGEKNPPLTVYDTSGPYTDPQASIDIRRGLPALRRAWIEERGDTR",
    "response#3": "MFPYPNSQKSYLLGSRPDIRVPVREITLSDTLVGGSKDAPIFEPNEPICVYDTSGVYTDPSHDIDLYKGLPKLREEWIEERRDA"
  },
  {
    "reference": "EYQSKKIMADHGVTVQRFFVADSANDALEAAQRLKAKEIVLKAQILAGGRGKGVFNSGLKGGVHLTKDPKIVEQLAKQMIGYNLSTKQTPKDGVTVKKVMVAEALNISRETYFAILMDRACNGPVMVGSPQGGVDIEEVAVTSPELIFKEEIDIFEGIKDHQALQMAKNLGFKGPLQQQAADQIKKLYNLFLKIDATQVEVNPFGETPEGQVVCFDAKINFDDNAEFRQKEIFAMDDKSENEPIENEAAKYDLKYIGLDGNIACFVNGAGLAMATCDIISLNGGKPANFLDLGGGVKEAQVYQAFKLLTADPKVEAILVNIFGGIVNCAIIANGITRACRELELKVPLVVRLEGTNVHEAQRILNESGLPIMSANDLEDAAKKAVASVAKK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have GTP binding, metal ion binding within mitochondrion to regulate tricarboxylic acid cycle, succinyl-CoA metabolic process.2. The GTP binding site should be located in a region of the protein that is accessible to the ligand.3. The protein should be designed to catalyze the following reaction CoA + GTP + succinate = GDP + phosphate + succinyl-CoA.4. The presence of ATP-grasp domains is critical for the interaction of the designed protein with its intended targets.5. A protein that can modulate the overall flux of metabolites through the tricarboxylic acid cycle pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MVNGAGLAMATMDIIKLNGAFPANFLDVGGGATTEKVTAAFKIILKDPAVEGILVNIFGGIMRCDTIAEGIVVAAKEVELDVPLVVRLEGTNVEKGKDILANSGLPIVPADDLGDAARKIVG",
    "response#2": "MVNGAGLAMGTMDMIQLAGGKPANFLDVGGTASPETVTEGFKIIMSDKNVKAILVNIFGGIVRCDRVAAGVIEAAKKVGLNLPVIVRLEGTNAEAAQTMLDESGLNLISANGLRDAAEFYEYSAKEIFRAEGISTPRGGVAETPEEAERIAAELGCDVAVKSQVLTGGRGKAGGIRFASPSGVAEVTGDLLSSEVRGETVEKVLIEEKIPIDRELYVSAVIDRTAKMPLIMASAEGGVDIEELAARSPEKIVRYHINPLDEFLPYEAREIARKMGLESELIPSVGGVIWKLYQLFRKYDARLAEINPLVGKDSRIMVNGAGLAMATMDVVNLFGGEPANFLDIGGGANAQKVAAALDIILDDPNVKVVMVNIFGGITRCDEVAKGIVEAQKIIKRQVPMVVRLVGTNEAEGQRILADASLTPASTLADAAQKGLEKLVELQ",
    "response#3": "MVNGAGLAMATMDIIKLNGAFPANFLDVGGGATTEKVTAAFKIILKDPAVEGILVNIFGGIMRCDTIAEGIVVAAKEVELDVPLVVRLEGTNVEKGKDILANSGLPIVPADDLGDAARKLL"
  },
  {
    "reference": "MRTLVLGGVRSGKSEYAEQLVAGFPSVRYLATGPTLSTDAEWTARIAAHRERRQERYETVESPDVTAALRQQPEVPVLVDDLGNWVAAQLDWDDDADPEQQARRLDGALAELCAAIAEHRADVVVVSPEVGMSVVPATTAGRRFQDLMGRANSTVATVADRVVLTVAGRALDLENRTADAGSHRAAPVAPLAAVGPASTETDAGAAIDPTDAELFPAVTPPDPAAAAAARDRQLRLTKPPGSLGRLEEVGIWVAACQGTSPPRPIASPTVVVFAGDHGVAAPDSDGGSVSAFPQAVTAQMVANFLAGGAAVNVLARRAGASVRVEDISVAADTAPEVSRHKVCRGSNDLRVTDAITLAQARQGVAAGRAVVDELVDSGVDLLIAGDMGIGNTTPASIVVGLFTHEEPVVVVGRGTGIDDAGWIRKTAAIRDGMWRGRKVIDDPLSLVAAVGGADFAAMAGFLAQAAVRRTPVILDGVVITAAALVADELAPGASEWWVAGHVSAEPAHKLALRRLGLTPLLDLDMRLGEASGALAALPLVTAATDLLGTMATFDEAGVSDG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.2. For general function, the protein need meet that Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB).3. A protein should be able to manipulate the activity of enzymes in the Nucleoside biosynthesis, alpha- ribazole from 5,6-dimethylbenzimidazole: step 1/2 pathway, either by activating or inhibiting their function.4. A protein with adenosylcobinamide kinase (ATP-specific) activity, cobinamide phosphate guanylyltransferase activity, nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity functions is required to enable phosphorylation. The designed protein sequence is ",
    "response#1": "MSVLSGRSSSSVETKERVTDPLAGVRVGLVGTSHRPYTWRIPDVLRELAARAGASPAAVTDLLVVPAGWPDGGPAVRAAAALGVPVVRSADGWEAWMAAHPGDGPDVVVVFGQVVAPAVLRPLLDAGATVIAWDRGAGGPRPGLAAPGAWDPAGLEAGDALPLLDELLDAGDHVVTIAGPGVPGAPEPDPTATGPDSSGATSPDPSGTTSPDPSGTTGPDPTGPTPANLSGPHPSDPSPVNPSGPIPARPSAVNPSGPIPAGPSPVRLVHSGPIRAGPATATGPDPTGPAPADLSGPSPGDPTVAAPHTLLLPRPLTPADLAALDPTGGPATAAPHTLLLPRPLTPADLAALDTTGGPAPAAPHTLLLPRPLTPADLAALDPTGGPATAAPHTLLLPRPLTPADLAALDPTGGPSTAAPHTLLLPRPLTPADLAALDPTGGPSTAAPHTVLLPRPLTPADLAALDPTGGPSTAAPRTVLLPRPLTPADLAALDPTGGPSTAAPHTVLLPHPLTPADLAALDPTGGPSTAAPHTMLLPRPLTPADLAALDPTGGPSTAAPHTMLLPRPLTPADLAALDPTGGPSTAAPHTMLLPRPLTPADLAALDPTGGPSTAAPHTMLLPRPLTPADLAALDPTGGPSTAAPHTMLLP",
    "response#2": "MTVIILSGGSGKRLDHDIPKQFVKLLGKPILVHSIEAFKKYSNCKEIIVVTSKDKVEKTKKILSEYELNDKIKFIIGGDTRQQSVFNGLKSVKNDCEVXIHDAARPFIDKSLILRIXNNXXSXGXAIPVIKVADSIKKVNKNXEILQTPDRSNLXQTQTPQGFKLKILLEAHKKATKXGFXVTDDAXLFEXFNIQXKIIEGSXTNIKITLPEDLEILEXXFNXKXENIXSXDXKX",
    "response#3": "MSILSGKVALVTGAARGQGRSHAVRLAAAGADVVVNDLGTSVTGEGPYPAATPEDTAETTALIEAEGRRAIAVHGSIADRAHVEAMVQQAIEVFGRLDILVNNAGILRDKSFAKMTAEDFQQVLNVHLTGTFYCTQAAWPHFSAQQYGRIVNTA"
  },
  {
    "reference": "MTSTPITYKTLADSIFNDLINNIIKQHTLTSLTNIKDHSSLLNSSNSNTNSNTNGTIASNGGNGTTSDENNEIENSTIQDKSKLKQLETSRYFRCLNCGRNIAGGRFASHISKCLERKRK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be involved in chromatin organization by localizing to DUBm complex, SAGA complex and exhibiting zinc ion binding, transcription coactivator activity.2. The protein should exhibit Polar residues compositional bias.3. The protein must exhibit the following characteristics: SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac); Within the SAGA complex, participates in a subcomplex, that specifically deubiquitinates histones H2B.; It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction and promoter selectivity, interaction with transcription activators, and chromatin modification through histone acetylation and deubiquitination; At the promoters, SAGA is required for recruitment of the basal transcription machinery; Functions as component of the transcription regulatory histone acetylation (HAT) complex SAGA; Involved in transcriptional regulation of a subset of SAGA- regulated genes. The designed protein sequence is ",
    "response#1": "MNNTVTFTPGMSVAFKLPYTRHNEGGDWIQCIIIKVTGEGAKQRFEVQDPEPDDDGNAGQIYKTTANHLIQIPAKGTPLPPISPKTNVLARYPETTTFYRAEVIRTLPDGSCKLRFEGEEEVGKETVVERHLVLEYNLFNDQEEEEEEDESADSMCNVCAQDITRSIHIRCVECVDFDLCIPEIQGYMPGRLEFDQEYMNEAELPIKDMNFDDDLHESAKHEMQLKLTMLNIYNSRLTRRAVRKQTIFNHNLLDYRRLQANEEDRQLLSEDEQALCSKLQIFPKPFLALKFALISASLTSKKPFQKTDAVNLFKHLDANKVEQVYDFFHNARWY",
    "response#2": "FTPGMSVAFKLPYTRHNEGGDWIQCIIIKVTGEGAKQRFEVQDPEPDDDGNAGQIYKTTANHLIQIDTGEIRWGTSDRQLLSEDEQALCSKLQIFPKPFLALKFALISASLTSKKPFQKTDAVNLFKHLDANKVEQVYDFFHNARWLNPKVKGPVDVEKQCGVLLPNGQMCARSLTCKTHSMSSKRAVPGRSQPYDVLLAACQKKNQVKIQRQDLTILSVSETIYHNLLTSMVQDIVSRTTSRQQLQDARYEKEKENGRSLDLRIKAREDDDRQLLSEDEQALCSKLQIFPKPFLALKFALISASLTSKKPFQKTDAVNLFKHLDANKVEQVYDFFHNARWLRDGDRQLLSEDEQALCSKLQIFPKPFLALKFALISASLTSKKPFQKTDAVNLFKHLDANKVEQVYDFFHNARWLTEDRQLLSEDEQALCSKLQIFPKPFLALKFALISASLTSKKPFQKTDAVNLFKHLDANKVEQVYDFFHNARWLSDGCSRDTSPCDQAPRYSLTILSVSETIYHNLLTSMVQDIVSRTTSRQQLQDARYEKEKENGRSLDLRIKAREDDKRRWPQKYHCNVCAQDITRSIHIRCVECVDFDLCIPCFTSGASLGTHHPSHPYRIIETNSLNPKVKGPVDVEKQCGVLLPNGQMCARSLTCKTHSMSSKRAVPGRSQPYDVLLAACQKKNQVKIQRQ",
    "response#3": "GMSVAFKLPYTRHNEGGDWIQCIIIKVTGEGAKQRFEVQDPEPDDDGNAGQIYKTTANHLIQIPAKGTPLPPISPKTNVLARYPETTTFYRAEVIRTLPDGSCKLRFEGEEEVGKETVVERHLVLEKEASKKKNPKVKGPVDVEKQCGVLLPNGQMCARSLTCKTHSMSSKRAVPGRSQPYDVLLAACQKKNSSSSGPIPQKYHCNVCAQDITRSIHIRCVECVDFDLCIPCFTSGASLGTHHPSHPYRIIETNSERDRQLLSEDEQALCSKLQIFPKPFLALKFALISASLTSKKPFQKTDAVNLFKHLDANKVEQVYDFFHNARWIS"
  },
  {
    "reference": "MATEIDINRIQKDIFIEHKGEPYRVLDYEHVKPGKGQAFVRVKAKNMLTGNVTELTFKASDRIPLADFEQVYATYSYNDGENYYFMNTQTYDMIAVPKEKIEEEAKFLKEGMEVIVFLYKGQPIGIELPKHVELQVVETEPAFKGDTQAGGTKPAKLETGAVIQVPFFVKEGDIVKVDTRTGSYVERVKEAK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Involved in peptide bond synthesis2. A protein that is capable of catalyzing reactions within the Protein biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "LFRGFPPERVQRVADGSRMVSFEPQEAMVEFGEDARFLGVLLEGEAARSVVSNSGRRQGLGRLQPGDVFGEMALMTGDKTTCDVIAQTRCRVLLVPVTLFSTVIMSEPQAVRRISKTIL",
    "response#2": "LFRGFPPERVQRVADGSRMVSFEPQEAMVEFGEDARFLGVLLEGEAARSVVSNSGRRQGLGRLQPGDVFGEMALMTGDKTTCDVIAQTRCRVLLVPVTLFSTVIMSEPQAVRRISKTIL",
    "response#3": "LFRGFPPERVQRVADGSRMVSFEPQEAMVEFGEDARFLGVLLEGEAARSVVSNSGRRQGLGRLQPGDVFGEMALMTGDKTTCDVIAQTRCRVLLVPVTLFSTVIMSEPQAVRRISKTIL"
  },
  {
    "reference": "MTKDQQNIERGSVINVEILNAAHGGQGIAKYDGRVIFVKGAFPGDRLSANITHVKKKFARATIASIEMPSPLRMQQRCLAAANGAGCCDFGEFDPEIEGRYKADLVLEQLERLGKVSQPPTCEVVSFGSPTQWRTRMRLGVDAQGRAGGFASQSREVVSGVPCSQGVMGLLDGIVGAEQNVLRFTPGSQVVVVCDDLGQRTVVETQPAPRGKRTESMVKVVEGTGKVSQVVDGVTFELPATGFWQSHKDAAQAYADTIREWFGSLIIRNTDSSLVAWDLYGGCGSFASAILSAVDNHGVVHCVESAPAAVSAGKRALSQLVEDQKIVFHTETVERAMNQLPAPTLVVLDPPRVGAGAETVRAIAQSGPQAAIHIGCDPATFARDIAEWSRNGFILEKLRVFDAFPGTHHCETIGLLTKKN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must have at least one TRAM domain.2. The S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions.3. The protein should be able to modulate RNA processing in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "STQVGCGYACTFCLTGTMGLERNLTAGEIVGQLLAANALLAGDGHRVTHLVFMGMGEPLANYAALVKAVRIFTDAKLGLGYSPRRITVSTVGLVTGIEKLGREDLKVNLAISLHAASDEVRSRLMPVNKSWNLAALMDAVRKYPLAHRQHVFFEYVMLDGVNDNVEDARRL",
    "response#2": "ITACVSTQVGCAMACQFCASGLLGLKRNLTAGEIVEQILHVKNHLLSGEHLTNVVFMGIGEPLANYDNVVKSVRIMNAEWGLGIGARHITISTVGIVDGIRRLAQERLKINLAISLHASDDITRSKIIPMNKNTGIKNLIAAAWEYFEITGRDVSFEYILINGINASKQDAESLAQLLKGMQCNINVLPVNPVEEFHWRPPSEGAIEIFCG",
    "response#3": "STQVGCGYACTFCLTGTMGLERNLTAGEIVGQLLAANALLAGDGHRVTHLVFMGMGEPLANYAALVKAVRIFTDAKLGLGYSPRRITVSTVGLVTGIEKLGREDLKVNLAISLHAASDEVRSRLMPVNKSWNLAALMDAVRKYPLAHRQHVFFEYVMLDGVNDNVEDARRR"
  },
  {
    "reference": "MQLARGTVGGRGCALFPLLSILVVQGARIVLSLEISADAHVRGYVGEKIKLKCTFKSSSDVTDKLTIDWTYRPPSSSRTESIFHYQSFQYPTTAGTFRDRISWAGNVYKGDASISISNPTLKDNGTFSCAVKNPPDVYHNIPLTELTVTERGFGTMLSSVALLSILVFVPSAVVVILLLVRMGRKATGVQKRSRSGYKKSSIEVSDDTDQEDSNDCMTRLCVRCAECLDSDYEEEAY",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in plasma membrane that is critical for extracellular matrix organization, cell adhesion, hair cycle.2. Incorporate a signal peptide in the protein design.3. The Extracellular, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.4. The designed protein should have at least Helical transmembrane domains to ensure proper localization.5. The presence of Ig-like V-type domains is critical for the interaction of the designed protein with its intended targets.6. The protein must exhibit the following characteristics: Mediates homophilic cell-cell adhesion.. The designed protein sequence is ",
    "response#1": "DFGGTTSFQCKVRSDVKPVIQWLKRVEYGSEGRHNSTIDVGGQKFVVLPTGDVWSRPDGSYLNKLLISRARQDDAGMYICLGANTMR",
    "response#2": "QEGLCVSVLCSFSYPQNGWTASDPVHGYWFRAGDHVSRNIPVATNNPARAVQEETRDRFHLLGDPQNKDCTLSIRDTRESDAGTYVFCVERGNMC",
    "response#3": "TPEPRSFEICSRQPEKQILSIVDKDLPPHTYPFKAALEHGSSNNWTVEIRGQDELAMGLKKELEPGEYNIFVKLTDSQGKAQVTQVKAQVCECEGTAKNCERRSYIVGGLGVPAS"
  },
  {
    "reference": "MSSITSRVSSRSSHELTEKKSGVTNDFKNSFEVGEVKRLPDAEGTADAVAQELLADDDFTYTAKEARRVLWKIDLVMMPVMCITYMIQYLDKTALSYAALYGMKTDTHIDGHTYSSMTTLFYAGYLVAQYPAAILMQKCRLSYFIFCNVFLWSAMVCLMAACRNGPSLLGLRFLAGIFEASITPAFINITAMWYRREEQPMRTLCWYAFNGIAQIIGSILSYGLGHIHGKVASWRYVFIVIGLMSLGWGVVFVFIPSNPSKARFLSSREKRIALERVRDNRTGLENKQFKWKHAYEAFLDPQVIMITLFTGVCMITNGIGVFSTLIIKGLGYNELHSAVLNMPLGAIEVAAMFISGVLCKVFKNGRLLIGVFMNCLTLAGCLMIWKIPDSNPYGRLVGVWFTMWVPASSALLLSLISSNVAGYTKKTVTSATVFVFYSVGNIVSPQLFKSGQTPEYIEGIQAMIVSLCIIIAIAFVLTGYYIYENKRRDRLLAEDPSLGESIKNEEFMDLTDRQQPKFRYRW",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization.2. A protein that contributes to dipeptide transmembrane transport by functioning in membrane, endoplasmic reticulum with dipeptide transmembrane transporter activity, transmembrane transporter activity. The designed protein sequence is ",
    "response#1": "LSMMQTLIHLLKCNIGTGLLGLPLAIKNAGLLVGPVSLLAIGVLTVHCMVILLNCAQHLSQRLQKTFVNYGEATMYGLETCPNTWLRAHAVWGRYTVSFLLVITQLGFCSVYFMFMADNLQQMVEKAHVTSNICQPREILTLTPILDIRFYMLIILPFLILLVFIQNLKVLSVFSTLANITTLGSMALIFEYIMEGIPYPSNLPLMANWKTFLLFFGTAIFTFEGVGMVLPLKNQMKHPQQFSFVLYLGMSIVIILYILLGTLGYMKFGSDTQASITLNLPNCWLYQSVKLMYSIGIFFTYALQFHVPAEIIIPFAISQVSESWALFVDLSVRSALVCLTCVSAILIPRLDLVISLVGSVSSSALALIIPALLEIVIFYSEDMSCVTIAKDIMISIVGLLGCIFGTYQALMD",
    "response#2": "MFALAFGFARSYWIPLSAAAVMLGTTVVFTLHRAIQRSAGTVIGVILAGAILFFKPGGVYIAFCIAALQCLLEMLIVRNYALAVPFLTANALVITESMHGGAGAGYFMIARLTDVAVGSVIGLF",
    "response#3": "MFALAFGFARSYWIPLSAAAVMLGTTVVFTLHRAIQRSAGTVIGVILAGAILFFKPGGVYIAFCIAALQCLLEMLIVRNYALAVPFLTANALVITESMHGGAGAGYFMIARLTDVAVGSVIGLF"
  },
  {
    "reference": "MSEEEKTTIPRVLVSADEFNKANEKLDEIEEKVEFTVGEYSQRIGQQIGRDIGILYGIVIGLIILAVTNILFAGLLKGLLKSLFGL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that is capable of catalyzing reactions within the methyl- coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2, One-carbon metabolism, methanogenesis from CO(2) pathway with high efficiency and specificity.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. A protein that can perform tetrahydromethanopterin S-methyltransferase activity in plasma membrane, methyltransferase complex to support methylation, methanogenesis, from carbon dioxide.4. For general function, the protein need meet that Part of a complex that catalyzes the formation of methyl- coenzyme M and tetrahydromethanopterin from coenzyme M and methyl- tetrahydromethanopterin The designed protein sequence is ",
    "response#1": "MGERKDYKKTATN",
    "response#2": "MTTTYQAPAAPAAPSRPAPRVALVPAAGSGRRMGADVPKAFVPLGGAPVLRWSLDALADHPSVDGVVVAVSDDQVGGFRAAAGTPVAGADGAAQTVPGAVRVVAGGRTRQDSVRLGLEAVRTDVXVXDAARALTPPAAFGRVLAAVDQGRAAGLLVPALPVTDTIKRVGADGSVVAVVDRSQLGAVQTPQGFPRTVLEQAYRAASEGGFTATDDAALVSAAGHPVVAVPGSEEAFKITTPADLDRAAALVRGGQR",
    "response#3": "MEIVLATRNQGKMKEIREILAPLGIRLLSLSDFPEIPEVVEDGLSFAENAIKKAREAVRATGLPAIADDSGLCVDALGGAPGIYSARFAGEPKSDERNNRKLIEELRGVPPERRTAHFTCAMALVDPEGEPHLVRGECEGRILEAPRGTGGFGYDPLFWVPEHNQTAAEIPAEAKNALSHRGRALRLFLERLC"
  },
  {
    "reference": "MSKISPLQKARSEYTPKLPPVLLECGPFAAARLGEPTQSVADQADIKRLFPDTYGLPAVTFVRGESFDCEPIKAGVILSGGQAPGGHNVIAGLFDGLKGLNPDSQLFGFLGGPSGLIDNKYIMITEELVDSYRNTGGFDMICSGRTKLETPEQFAKVTANCKQHGITAIIIIGGDDSNTNACTLAQYMLTEKTGIRVIGCPKTIDGDLKNEWIEVSFGFDTATKVYSELIGNIMRDANSARKYWHFIKLMGRSASHIALECALQTQPNACIISEEVAAKKLSLEEIVTRLTELVVKRAEAKMNFGVVLIPEGLIEFIPEINVLIAELNELLAKEATAFNALADMSAKIDFVAKHLSAAAVGAFKSLPTMLKAQLLGDRDPHGNVQVSRIETEKLLITMVEERLAKINAGRETPIKFSTQAHFFGYEGRCAAPSNFDADYCYSLGYTAAALLGAGKTGYMASVRNVFSPACEWVAGGIPLTMMMNIERRHGKDVPVIRKALVELDGKPFKTFAANRDAWGVGVEYVYPGPIQYFGPADVCDRVTMTLALEHA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a specific function within the cytoplasm.2. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate, Mg(2+) binding.3. A protein should be able to manipulate the activity of enzymes in the D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 3/4 pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "AQSGGVTAVINASAAGVIEAARKQSGKIGRIYAGRNGIIGALTEDLIDTGQESDAAISALRYTPSGAFGSCRYKLKSLEQNRREYERLIEVFKAHDIGYFFYNGGGDSADTCLKVSQLSGTLGYPIQAIHVPKTVDNDLPITDCCPGFGSVAKYIAVSTLEASFDVASMSATSTKVFVLEVMGRHAGWIAAAGGLASSPEREIPVVILFPEISFDKQKFLAKVDSCVKKFGYCSVVVSEGVKGDDGKFLSDQGVRDAFGHAQLGGVAPVVASMVKEGLGLKYHWGVADYLQRAARHIASKTDVEQAYAMGQAAVEFILQGKGGRCVGIQNEKIGILTGGGDCPGLNPAIRGAVMRGADFGFEFLGIQEGWRGLVEGNAEPLKVSDVFEIVGKGGTVLGTSRTNPFKKDGAVQKCLDTFKKLKLDALIACGGEDTLGVASKLFKEHKVPVVGVPKTMDNDLSATDYTFGFDTATTIAVDALERLRDTGRSHRRVMVLEVMGRHAGWVALFTAIAGAADWVCLPEEPVDVKAMCENVKKAHAKRKTALVVTSEAVTIPGEAVGKEALDDFGHMLLRKRGVGDRIAGIIESETKLETRAAVIGHIQRGGAPTLFDRILGTRVGAKAAEWILQGKGGRCVGIQNE",
    "response#2": "VVGQSGGPTAVINASLYGTVYEALNREDEIGTVYGMINGIEGFLNDQVMDMAPLEESKELELIRTTPGSYLGSCRYKLPEDLNDPVYPQLFARFEAYNIGYFFYIGGNDSMDTVSKLSRYAEKISSDIRVIGIPKTIDNDLVETDHTPGFGSAAKYVASTVREIAIDASVYDNKKSVTIVEIMGRHAGWLTAASALARKFEHDNPVLIYLPETDFDQDAFIEKVRTSLETTPNLVVCISEGIHDNTGTFICEYSNDVGTDTFGHKMLTGSGKYLENLVKERLGVKVLS",
    "response#3": "VVGQSGGPTAVINASLYGTVYEALNREDEIGTVYGMINGIEGFLNDQVMDMAPLEESKELELIRTTPGSYLGSCRYKLPEDLNDPVYPQLFARFEAYNIGYFFYIGGNDSMDTVSKLSRYAEKISSDIRVIGIPKTIDNDLVETDHTPGFGSAAKYVASTVREIAIDASVYDNKKSVTIVEIMGRHAGWLTAASALARKFEHDNPVLIYLPETDFDQDAFIEKVRTSLETTPNLVVCISEGIHDNTGTFICEYSNDVGTDTFGHKMLTGSGKYLENLVKERLGVKVVVGQSGGPTAVINASLYGTVYEALNREDEIGTVYGMINGIEGFLNDQVMDMAPLEESKELELIRTTPGSYLGSCRYKLPEDLNDPVYPQLFARFEAYNIGYFFYIGGNDSMDTVSKLSRYAEKISSDIRVIGIPKTIDNDLVETDHTPGFGSAAKYVASTVREIAIDASVYDNKKSVTIVEIMGRHAGWLTAASALARKFEHDNPVLIYLPETDFDQDAFIEKVRTSLETTPNLVVCISEGIHDNTGTFICEYSNDVGTDTFGHKMLTGSGKYLENLVKERLGVKVERVEEGGDEVVIYRIGVLTGGGDCPGLNAVIRAVTRKGIEVHGKEIVGFRSGWRGPLEGLTMPLGLEEVERILDRGGTILGSSRTNPYKEADGVARIKRTLEEHGIDALVAIGGEDTLGVANGLFEEGVPVVGVPKTIDNDLDATDYTFGFDTAVHIATEAVDRLRTTAESHHRALVVEVMGRHAGWIALHSGLAGGANVILGPEQRFSVDWVCDWVTRRFERQYAPIIVVAEGAIPEGGAEVLQGGERDAFGHVRLGGVGTWLAEEIAERTGNESRAVVLGHTQRGGTPTAYDRVLATRFGLHAMDA"
  },
  {
    "reference": "MGGKWSKSSIVGWPEVRERIRRTQTPTASAAIGVGAVSQDLDKHGAITSSNTAATNADCAWLEAQEDEEVGFPVKPQVPLRPMTFKGALDLSHFLKEKGGLEGLIYSKKRQEILDLWVYNTQGYFPDWQNYTPGPGPRFPLTFGWCFKLVPMDPADVEEATEGENNSLLHPICQHGMEDEDREVLVWRFDSRLAFTHVAREKHPEFYKDC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1.2. Incorporating a Dileucine internalization motif; necessary for CD4 internalization, PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif is critical for the successful design of the protein.3. A protein that localizes to virion component, host cell plasma membrane, extracellular region, host cell Golgi membrane and can perform GTP binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPAIRERIRRTEPAAEGVGAASQDLEKHGALTSSNTPTNNAACAWLEAQEEAEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLVWSRKRQDILDLWVYNTQGYFPDWHNYTPGPGIRYPLTFGWCFKLVPVEPEEVEKANEGENNSLLHPMSLHGMEDPEKEVLVWRFDSRLAFHHVAREKHPEYYKDC",
    "response#2": "MGGKWSKSSLVGWPAVRDRIRRTEPAADGVGAASRDLEKYGALTSSNTPATNAACAWLEAQEEEEEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLDGLIYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVDPDKVEEANEGENNCLLHPICQHGMEDEHREVLKWKFDSSLARRHLARELHPEFYKDC",
    "response#3": "MGSKWSKSSIVGWPSVRERMRRTRAGEXAAEGVGAASQDLDKYGALTSSNTATNNAACAWLEAQEEDEGVGFPVRPQVPVRPMTYKGAFDLSFFLKEKGGLDGLIYSKKRQEILDLWVYNTQGXFPDWQNYTPGPGTRFPLTFGWCFKLVPVDPREVEEANKGENNCLLHPMSQHGMEDEHREVLKWRFDSSLARRHIARELHPEYYKDC"
  },
  {
    "reference": "MKLQFKPVYLASIAIMAIGCTKEVTENDTSEISEVPTELRAAASSFYTPPGQNVRANKKNLVTDYGVNHNDQNDDSSKLNLAIKDLSDTGGILTLPKGKYYLTKIRMRSNVHLEIEKGTVIYPTKGLTPAKNHRIFDFASKTEEKIENASIVGKGGKFIVDLRGNSSKNQIVADVGNVTNFKISNFTIKDEKTIFASILVSFTDKAGNAWPHKGIIENIDQANAHTGYGLIQAYAADNILFNNLSCTGGVTLRLETDNLAMKTAKKGGVRDIFATKIKNTNGLTPVMFSPHFMENGKVTIDDVTAIGCAYAVRVEHGFIEIFDKGNRASADAFKNYIEGILGAGSVEVVYKRNNGRTWAARIANDFNEAAYNHSNPAVSGIKPGKFATSKVTNVKATYKGTGAKLKQAFLSYLPCSERSKVCRPGPDGFEYNGPSLGVTIDNTKRDNSLGNYNVNVSTSSVQGFPNNYVLNVKYNTPKVCNQNLGSITSCN",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should aim to mimic or enhance the function of natural proteins involved in polysaccharide catabolic process, potentially with improved properties such as stability, specificity or selectivity.2. The protein have enhanced Endohydrolysis of 1,4-beta-D-linkages between D-galactose 4-   sulfate and 3,6-anhydro-D-galactose-2-sulfate in iota-carrageenans.. The designed protein sequence is ",
    "response#1": "DMVIKLKQELIMNSFKTIDARGANVHIANGACITIQNITNVIVHGLHIHDCKRTGNVTVRSSPSQAGFRGTADGDAINIFGSSHIWIDHNSLSNCTDGLVDVVNGSTAITISNNHFTHHDEVMLLGHNDSYTRDKMMQVTVAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWKMYAIGGSANPTINSQGNRFAAPKNC",
    "response#2": "DMVIKLKQELIMNSFKTIDARGANVHIANGACITIQNITNVIVHGLHIHDCKRTGNVTVRSSPSQAGFRGTADGDAINIFGSSHIWIDHNSLSNCTDGLVDVVNGSTAITISNNHFTHHDEVMLLGHNDSYTRDKMMQVTVAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWKMYAIGGSANPTINSQGNRFAAPKNC",
    "response#3": "DMVIKLKQELIMNSFKTIDARGANVHIANGACITIQNITNVIVHGLHIHDCKRTGNVTVRSSPSQAGFRGTADGDAINIFGSSHIWIDHNSLSNCTDGLVDVVNGSTAITISNNHFTHHDEVMLLGHNDSYTRDKMMQVTVAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWKMYAIGGSANPTINSQGNRFAAPKNC"
  },
  {
    "reference": "MIELKGRKLVLGVTGGVAAYKAAELVRLLGKAGADVHVVLTEGGARFVTAVTFQALSGNPVWTDLWDPRMANNMAHIDLTRDADAILIAPATADVMAKLVHGLADDLLTTLCLARDCPLLVAPAMNRQMWEHPATRRNALQLAADGVSLLGPAAGEQACGEVGLGRMLEPEALCEAVIAFFQPKLLAGRRIVMTAGPTFEPIDPVRGITNSSSGKMAYGLARACVQAGADVTLVSGPVALPVPDGAQRVSVRSALEMREVVFAALPGADVFIGVAAVADYRPAAAAEHKIKKSGAELAITLTPNPDILAEVAGLPKPPFCVGFAAESRNLDEYAEGKRKAKKLPMVVGNLVQDGLGGDDNEVVLYDEAGRHPVPKAPKPEVARAIVAHLAALLPES",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein have enhanced H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.2. A protein with phosphopantothenate--cysteine ligase activity is needed to enhance pantothenate catabolic process in a specific context.3. The designed protein must have at least one Flavoprotein domain.4. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.5. The designed protein should have a high specificity for CTP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MQIVLGVAGGIAAYKAVLLLRLLREAGHDVRVVPTRAALEFVGRPTWEALSGQSATPEVFDDVAHVPHVALGQHADLVIVAPATADLLARAAAGRADDLLTSTLLTARCPVLMAPAMHTEMWDHPATRANVATLRARGVHVLDPASGRLTGADTGPGRLPEPDQIAAAVELLLAR",
    "response#2": "MQIVLGVAGGIAAYKAVLLLRLLREAGHDVRVVPTRAALEFVGRPTWEALSGQSATPEVFDDVAHVPHVALGQHADLVIVAPATADLLARAAAGRADDLLTSTLLTARCPVLMAPAMHTEMWDHPATRANVATLRARGVHVLDPASGRLTGADTGPGRLPEPDQIAAAAELLLG",
    "response#3": "MAGKRVMVTLGPTREAWDGVRFWSNPSTGLMGAALGVAAWLRGAEVAVVCGPGVRTRLPREITRVDVVSARDMFEAASDLWPGMDMGMFTAAVADFSPKPFGARKFKKADAPDGLTVSFTPNPDILHTLSHGRKPGQKVLGFAAETAADMQALLPLAHAKRQRKNADVLAANRVNTTDSGFGAATNSMALD"
  },
  {
    "reference": "MAALLLLLPLLLLLPLLLKLDVWPQLRWLPADLAFTVRALRCKRALRARALAAAAADPESSESGCSLAWRLAYLAREQPTHTFLIHGAQRFSYAEAERESNRIARAFLRARGWTGGRRGSGRGSTEEGARVAPPAGDAAARGTTAPPLAPGATVALLLPAGPDFLWIWFGLAKAGLRTAFVPTALRRGPLLHCLRSCGASALVLATEFLESLEPDLPALRAMGLHLWATGPETNVAGISNLLSEAADQVDEPVPGYLSAPQNIMDTCLYIFTSGTTGLPKAARISHLKVLQCQGFYHLCGVHQEDVIYLALPLYHMSGSLLGIVGCLGIGATVVLKPKFSASQFWDDCQKHRVTVFQYIGELCRYLVNQPPSKAECDHKVRLAVGSGLRPDTWERFLRRFGPLQILETYGMTEGNVATFNYTGRQGAVGRASWLYKHIFPFSLIRYDVMTGEPIRNAQGHCMTTSPGEPGLLVAPVSQQSPFLGYAGAPELAKDKLLKDVFWSGDVFFNTGDLLVCDEQGFLHFHDRTGDTIRWKGENVATTEVAEVLETLDFLQEVNIYGVTVPGHEGRAGMAALALRPPQALNLVQLYSHVSENLPPYARPRFLRLQESLATTETFKQQKVRMANEGFDPSVLSDPLYVLDQDIGAYLPLTPARYSALLSGDLRI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design must be able to perform long-chain fatty acid-CoA ligase activity in order to contribute to long-chain fatty acid metabolic process.2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.3. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding. The designed protein sequence is ",
    "response#1": "QPYKWLSYKQVSDRAEYLGSCLLHKGYKPSPDQFVGIFAQNRPEWIISELACYTYSMVAVPLYDTLGAEAIIYIINKADIATVICDTPQKASVLIGNVEKGLIPGLKMVILMDPFDDNLKQRGEKTGVEILALSDAENLGKENFSKPVPPKPEDLSIICFTSGTTGDPKGAMITHENIASDVAGFLKCMEHNFVPSPNDVTISYLPLAHMFERIVQATIYSCGARVGFFQGDIRLLPDDMKTLKPTVFPAVPRLLNRLYDKVQNEAKTPLKKFFLNLAIARKFEEVKRGIIRRDSFWDKVIFAKIQDNLGGKVRIMVTGAAPISSPVLTFLRAAMGCAVHEAYGQTECTGGCTFTLPGDWTSGHVGTPMACNYVKLEDVVDMNYFSANNEGEICIKGPNVFKGYWNNPVKTKESFVDGWYRTGDIVTVDKDGYVSIVDRKKELIKYKGYQVPPAEIEEFLYQHPAVLDVQVVGVPDAKFGEEAAAWIKLKDGKSVSPDELKAYCKGKIARHKIPRYVIFTDDYPMTASGKILRKDLRAKLTASM",
    "response#2": "QPYKWLSYKQVSDRAEYLGSCLLHKGYKPSPDQFVGIFAQNRPEWIISELACYTYSMVAVPLYDTLGAEAIIYIINKADIATVICDTPQKASVLIGNVEKGLIPGLKMVILMDPFDDNLKQRGEKTGVEILALSDAENLGKENFSKPVPPKPEDLSIICFTSGTTGDPKGAMITHENIASDVAGFLKCMEHNFVPSPNDVTISYLPLAHMFERIVQATIYSCGARVGFFQGDIRLLPDDMKTLKPTVFPAVPRLLNRLYDKVQNEAKTPLKKFFLNLAIARKFEEVKRGIIRRDSFWDKVIFAKIQDNLGGKVRIMVTGAAPISSPVLTFLRAAMGCAVHEAYGQTECTGGCTFTLPGDWTSGHVGTPMACNYVKLEDVVDMNYFSANNEGEICIKGPNVFKGYFNKPEATAESFEPGGWFRTGDLGYIREDGYIWLVDRKKDMIISGGENIYPSEIEEFLYQHPAVLDVQVVGVPDAKFGEEAAAWIKLKDGKSVSPDELKAYCKGKIARHKIPRYVIFTDDYPMTASGKILKRILRERE",
    "response#3": "QPYKWLSYKQVSDRAEYLGSCLLHKGYKPSPDQFVGIFAQNRPEWIISELACYTYSMVAVPLYDTLGAEAIIYIINKADIATVICDTPQKASVLIGNVEKGLIPGLKMVILMDPFDDNLKQRGEKTGVEILALSDAENLGKENFSKPVPPKPEDLSIICFTSGTTGDPKGAMITHENIASDVAGFLKCMEHNFVPSPNDVTISYLPLAHMFERIVQATIYSCGARVGFFQGDIRLLPDDMKTLKPTVFPAVPRLLNRLYDKVQNEAKTPLKKFFLNLAIARKFEEVKRGIIRRDSFWDKVIFAKIQDNLGGKVRIMVTGAAPISSPVLTFLRAAMGCAVHEAYGQTECTGGCTFTLPGDWTSGHVGTPMACNYVKLEDVVDMNYFSANNEGEICIKGPNVFKGYWKDPAKTEAAFKGDYWFRTGDLGSVDEDGFCTIAGRIKEVEAVLSRLLGQTDVAVYGVAVPGVEGKAGMAAIADPHSQLDPNSMYQELQKVLASYARPIFLRLLPQVDTTGTFKLRRVELKNKLAEM"
  },
  {
    "reference": "MTEKLGILAIGHGSKLPYNKEVVTQIANYIAQKHSDVVVRAGFMENSEPTLEEAIEGFSGTGVTKISAVPVFLASGVHITKDIPEILSLDKEGCGILEIDGKEVPLCYAKPLGADELIAELVFKRVQESL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Ni(2+), substrate, Co(2+) binding.2. The protein must exhibit the following characteristics: Catalyzes the insertion of Co(2+) into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis; Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation.3. A protein should be able to manipulate the activity of enzymes in the cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 1/10 pathway, either by activating or inhibiting their function.4. The protein should have a low Km value for 2 H(+) + Ni-sirohydrochlorin = Ni(2+) + sirohydrochlorin.5. The designed protein must possess sirohydrochlorin cobaltochelatase activity, cobalt ion binding, nickel cation binding to support methanogenesis under certain conditions. The designed protein sequence is ",
    "response#1": "MLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDIILVGAGPGDSGLLTLRGLQILQQADVVLYDCLVSQDILDLIRRDAKRIYVGKRAGLKSITQDKIIKLLIFLARQGKKVVRLKGGDPFIFGRGGEEIEAAKNAGIDFQVIPGITSAIGIAAYTGIPLTHRKYAQGVIFITGHKCKNGLLNNFSILCDPSYTLVVYMATLHAVEISQKLIMHGRLKSTPIAIVGKGTTVNQKVIIGRLDEVGAGPGDSGLLTLRGLQILQQADVVLYDCLVSQDILDLIRRDAKRIYVGKRAGLKSITQDKIIKLLIFLARQGKKVVRLKGGDPFIFGRGGEEIEAAKNAGIDFQVIPGITSAIGIAAYTGIPLTHRKYAQGVIFITGHKCKNGLLNNFSILCDPSYTLVVYMATLHAVEISQKLIMHGRLKSTPIAIVGKGTTVNQKVIIGRLDEIKKMINFSITPSLLIIGEVDNYENMKIGEVYLVGDGPGDPDLLTFRALRLMQQADVVLYDRLVSPDVLDLVRRDAELIFVGKEPTVHPVPQEKINQLLIRLAKEGRRVLRLKGGDPFMFGRGGEEIETLMDEGIPFQVVPGITAATGTSAYAGIPLTHRDYAQSCIFVTGHLKNDSTNLNWNVLAQPHQTVVIYMGLRSLAVIARELIQHGMPAEKPAALVQKATTADQRVLVGTLETLPGIVEAADIKLSSIIIIGDVVKL",
    "response#2": "MLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDGEIILVGAGPGDSGLLTLRGLQILQQADVVLYDCLVSQDILDLIRRDAKRIYVGKRAGLKSITQDKIIKLLIFLARQGKKVVRLKGGDPFIFGRGGEEIEAAKNAGIDFQVIPGITSAIGIAAYTGIPLTHRKYAQGVIFITGHKCKNGLLNNFSILCDPSYTLVVYMATLHAVEISQKLIMHGRLKSTPIAIVGKGTTVNQKVIIGRLDEIKKMINFSITPSLLIIGEVVLKIGVAVDEAFNFYYNDVFDILGAKGAEIVTFSPIHDSLPEADGYILGGGYPEMFAGELEANDRMREAVLEVSRNGTPIYAECGGLIYLTGELVLKSGWNDLEADKSYGMCGVFDGKTVMPSKRVIGYVKGVSDSRSPLGEAVFSGHEFHHTDVRLPKDTHYSYRLSRGSGIIGGFDGAVANRTQGSYTHLHPG",
    "response#3": "MLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDVYLVGDGPGDPDLLTFRALRLMQQADVVLYDRLVSPDVLDLVRRDAELIFVGKEPTVHPVPQEKINQLLIRLAKEGRRVLRLKGGDPFMFGRGGEEIETLMDEGIPFQVVPGITAATGTSAYAGIPLTHRDYAQSCIFVTGHLKNDSTNLNWNVLAQPHQTVVIYMGLRSLAVIARELIQHGMPAEKPAALVQKATTADQRVLVGTLETLVLTGANSVYLVGDGPGDPDLLTFRALRLMQQADVVLYDRLVSPDVLDLVRRDAELIFVGKEPTVHPVPQEKINQLLIRLAKEGRRVLRLKGGDPFMFGRGGEEIETLMDEGIPFQVVPGITAATGTSAYAGIPLTHRDYAQSCIFVTGHLKNDSTNLNWNVLAQPHQTVVIYMGLRSLAVIARELIQHGMPAEKPAALVQKATTADQRVLVGTLETL"
  },
  {
    "reference": "MNFENVTAVAKANVYFDGKVVSHSVLFGDGSKKTLGLIYPGEYHFGTDAAEEMAITDGTCEVMLDGQDTTATYEAGTAFEVAAKSGFTIRVKEGICQYVCSFLS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein have enhanced phosphate + xanthosine = alpha-D-ribose 1-phosphate +   xanthine. The designed protein sequence is ",
    "response#1": "VGHAFATRFRDQGITKIVTIEASGIAPAVMAGLELGVPVIFARKYQSLTLKDNLYISKVFSFTKQTESTIAISAKHLNAHDHVLVIDDFLANGHAAKALIDLIGQAGASIAGLGIVIEKSFQDGRALLESEGYRVESLL",
    "response#2": "MGNRFAEVFAEAGITKVITIEASGIAPALYAAQKLGVPMIFARKAKSLTMDEELLTASVYSFTKQVTSQISISRKFLSDADKVLIIDDFLANGQAAKGLVELCQQAGAKVEGIGIVIEKSFQDGRQLLEDMGLNVVSLVR",
    "response#3": "MGNRFAEVFAEAGITKVITIEASGIAPALYAAQKLGVPMIFARKAKSLTMDEELLTASVYSFTKQVTSQISISRKFLSDADKVLIIDDFLANGQAAKGLVELCQQAGAKVEGIGIVIEKSFQDGRQLLEDMGLNVVSLL"
  },
  {
    "reference": "MTRYIFVTGGVVSSLGKGIASASLAAILEARNLKVTILKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFIRTRMTRKNNFTTGRVYETVLRKERRGDYLGGTVQVIPHVTDEIKRRIVEGAGNADVAIVEVGGTVGDIESLPFLEATRQLKLEVGSDRAMLMHLTLVPYIATAGEVKTKPTQHSVKELRSIGLQPDILICRSEQPIDQSARRKIALFTNVEQRAVISLEDAKTIYNIPKMLHAQGLDEFIVERMRLKCEPADLSEWQAVVDAQTFPQHEVTIAMVGKYMELLDAYKSLNEALMHAGIQTRTRVNINYIDSESIQQQGTEALEGADAILVPGGFGERGIEGKLQAVRFARENKVPYLGICLGMQVAVIEYARNVAGMPTANSTEFNHDTEYPVVGLIAEWVDASGKTEIRNVDSDLGGTMRLGTQDCSLVEGTHVRNCYGSATIRERHRHRYEVNNNLLPDLEKAGLTVAGYSSNTDSNGKALVEVIEVRDHPWFVACQFHPEFNSTPRDGHPLFQAFVEAALAYHEAKE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.2. The protein should be able to bind Mg(2+), L-glutamine ligand in a variety of conditions3. The designed protein must have at least one Glutamine amidotransferase domain.4. The protein must exhibit CTP synthase activity, metal ion binding. The designed protein sequence is ",
    "response#1": "LKDAYKSLTEALTHGGIANNVRVNLEWIEAEVFEREDPAPFLEGLHGILVPGGFGQRGAEGKIRAARYARERNIPYFGICFGMQMAVIEAARSLAGITDANSTEFGETREPVVGLLTEWMRGNELERRAAESDLGGTMRLGAYKATLAEGTKIAQMYGDTEISERHRHRYEVNMAYRECLEAKGLRFSGTSPDGLLPETVEHEGHPWFIGVQFHPELKSRPFEPHPLFKGFIY",
    "response#2": "LKDAYKSLTEALTHGGIANNVRVNLEWIEAEVFEREDPAPFLEGLHGILVPGGFGQRGAEGKIRAARYARERNIPYFGICFGMQMAVIEAARSLAGITDANSTEFGETREPVVGLLTEWMRGNELERRAAESDLGGTMRLGAYKATLAEGTKIAQMYGDTEISERHRHRYEVNMAYRECLEAKGLRFSGTSPDGLLPETVEHEGHPWFIGVQFHPELKSRPFEPHPLFKGFIY",
    "response#3": "DSYKSLKEALVHGALAHNLKLRVTWIEAEGLETENYEQQLAGLDGILVPGGFGKRGIEGMLNAIRYAREQQVPYFGICLGMQTACIEYARNVCGLKGANSGEFDPATPHRIIYKLRELTGVEEMGGTMRLGAWACVLEPGSLAAKAYGTTEISERHRHRYEFNREYEAVLTGGGLRLTGTTPDATYVEIVEIPDHPFFLGCQFHPEFKSKPLEPHPLFRDFL"
  },
  {
    "reference": "MAGDRNRHCELEQEKYDTHENVKIICLGDSAVGKSKLMERFLMDGFQPQQLSTYALTLYKHTATVDGKTILVDFWDTAGQERFQSMHASYYHKAHACIMVFDVQRKITYKNLGTWYAELREFRPEIPCILVANKIDADIQMTQKNFSFAKKFSLPLYFVSAADGTNVVKLFNDAIRLAVAYKESSQDFMDEVLQELENFKLEQKEEDTSGQEQSDTTKSPSPS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The GTP binding site should be stable and able to withstand changes in environmental conditions.2. The protein should have a specific function in flagellated sperm motility, intracellular protein transport and should be localized to ciliary basal body, endomembrane system in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "LNIEGLKYKERDGFEMAFTFWQPQPWNVFEVTAYWEQRSRALLGTRYDHRNGAFDWDLSMTLKERGGQQICSQEYRYWRETGVAFVFPEYEHCKPNKTLAVGLVRNGRNFIHRGYVGDIQTGPFCGFGLRTVEERMHHSVHGDNDYRATDITERNLMEFFHELLTQTAYEHDTTRSRRYGSVQLLMTPLLNHQEEDAAGQAAYDKPWIHVPGVTVHFVSPMEMEQLKKGAAHWNNMFDIVFMAYNYYSFLSKDFFQAMRAQSLFILETKLMTVERKDRVQEYESKAKELMKDAGLKL",
    "response#2": "RERCEELRTKLASIHEKKVVEERNAQIEFNKELKRQKLVEEHLFARLWEEDRLAKERREAQEEKRQRELVQNTRLGLDAQVTSIQAQRQGARRMKEEEARILEQNKAQIKREDEQEKLQKQKRRQETRSSLKKAVQDKIESMQREYREDLDLNMKLVGRALQDLQDEADKKKQKREEMGREQKIYNDYLMQRREEEKAQEKELNRLLEDIKAKKLAEKDRELALQRAARKQLMNEVMNTRKLQVQERLQRKLREQEELALHEQRISESLKVLHQEDMEDFARRCALAEEYRNQLQMQIAHQQQAREAEKEEERQEFEAGLAANKACLDKIQRC",
    "response#3": "RERCEELRTKLASIHEKKVVEERNAQIEFNKELKRQKLVEEHLFARLWEEDRLAKERREAQEEKRQRELVQNTRLGLDAQVTSIQAQRQGARRMKEEEARILEQNKAQIKREDEQEKLQKQKRRQETRSSLKKAVQDKIESMQREYREDLDLNMKLVGRALQDLQDEADKKKQKREEMGREQKIYNDYLMQRREEEKAQEKELNRLLEDIKAKKLAEKDRELALQRAARKQLMNEVMNTRKLQVQERLQRKLREQEELALHEQRISESLKVLHQEDMEDFARRCALAEEYRNQLQMQIAHQQQAREAEKEEERQEFEAGLAANKACLDKIQRC"
  },
  {
    "reference": "MLKGKTVVLGVTGSIAAYKIAYLASALVKLHADVNVIMTQNATNFINPITFETLTSHKCLVDTFDRNFQYNVEHVALAKRADIFMVAPASANVIGKMANGIADDMLTTTILAAKCPKLVSPAMNTNMYTNQIVQDNLEKLKHYGFEIIDPANGYLACGDTGAGKMPEPETLLQYILRELASEKDLAGKRVLITAGPTQEKIDPVRYITNHSTGKMGYALAECCMRRGAEVTLVSGPVAIQPPMFVDVVPVTSAEDMAREVKTRADSQDIIIKAAAVADYRPVNPADEKIKKKDGDSSIELERTEDILKYLGEHRKQGQFICGFSMETEHMVENSTAKLDKKNVDMIVANNLKVSGAGFGTDTNIVTLITRAGAKELPIMSKSEVADAIMDEILTQIEK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A2. The designed protein must have at least one Flavoprotein domain.3. The designed protein must have a Proton donor active site that is highly conserved among related enzymes.4. A protein with FMN binding is needed to enhance coenzyme A biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "MRIVLGIGGGIAAYKAALLLRLFTEAGHDVTAVPTEAALAFVGKATWEALSGKPVRTDVFEAVETVNHVRLGHEAELVVVAPATADLLARAAGGHADDLLTNTLLMARCPVLLAPAMHTEMWRHPATQANVALLRERGVTVLDPAVGRLTGPDTGPGRLPEPEDIFDALG",
    "response#2": "KHIVLGVTGSIAAYKIASLASMLVKKQADVTVIMTKNATNFINPITFESLTGNRCLVDTFDRNFEFQVEHVSLAKQTDVFLVAPASANVIAKAAHGIADDMLTTTLLACRCPKIFAPAMNTRMYENPIVQDNISTLKDYGMEVVTPASGYLACGDTGEGKMPEPEVLYEAVLRKHIVLGVTGSIAAYKIASLASMLVKKQADVTVIMTKNATNFINPITFESLTGNRCLVDTFDRNFEFQVEHVSLAKQTDVFLVAPASANVIAKAAHGIADDMLTTTLLACRCPKIFAPAMNTRMYENPIVQDNISTLKDYGMEVVTPASGYLACGDTGEGKMPEPEVLYEAVLRASRALAEGPLSGRKVLVTAGPTQEKLDPVRYISNHSTGKMGYAVAAAAVRRGADVTLVSGKVELTPPMGVQVVPVVSAADMAQAVKAAASEQDIIIKAAAVADYRPRVTADEKLKKKDEELSVELERTEDILAWLGAHRRAGQILCGFSMETEHLLENSRVKLEKKKIDMIVANNLKQEGAGFGTDTNVVTLITASGEEDWPRMSKEAVGERLAARIAEALG",
    "response#3": "LKGKKVIVSAAGTREPIDPVRYISNRSSGKMGYAIATEAASRGAEVVLVSGITNIKAPENIKLIRVESAREMQKHIEEHFADCDITIMSAAVSDYRVKEVASQKIKKSDEVMTIELVKNPDILKGLGEKKSDKQYLVGFAAETQNVEEYAKGKLQRKNLDMIVANDVSQAGAGFNVDTNIAKIE"
  },
  {
    "reference": "MQTVNIVILAAGQGKRMNSSLPKVLHPVSTKPMLQHVIDTALELSPERLIVVYGHGGDQVQNTLLPIYGDKIQWAHQDQQLGTGHALKCALPYLSNSGVTLVLYGDVPLISLATLQKMLAKYQDSIVMLTDEVDNPAGYGRVVRNSEFAITGIIEEKDASQSERLIREINTGFYVLPNKKLADWLSRLSNNNNQNEYYLTDVIGLAYQDGVEIDYVLSPHHYEVLGVNNKLQLEQLERIYQLNRANKLLEAGVTLYDKTRIEIRGSIKAGKDCCVDVNCIFEGDVVLGDNVTIGAGCIIKNATIADNVTIKPYTIIEEARISAGAQIGPYARLRPGTELAEDTHVGNFVEIKKSQVGKGSKINHLTYIGDANIGSKVNVGAGSVTCNYDGQNKFKTVIGDNVFVGSGTMLVAPVSLKNGSLIGAGSVITEDTPENELTLARTKQVTVYGWKKRNK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein requires Mg(2+) to activate a critical catalytic site.2. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain..3. The protein should have a high affinity for UDP-N-acetyl-alpha-D-glucosamine.4. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.5. The designed protein should be involved in regulation of cell shape, lipid A biosynthetic process, cell morphogenesis, peptidoglycan biosynthetic process by localizing to cytoplasm and exhibiting glucosamine-1-phosphate N-acetyltransferase activity.6. Ideal protein should have a binding affinity for key enzymes in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "VLVLAAGEGTRMRSATPKVLHTLGGRSMLGHALTAARELEPGRVVVVVRHAREQVVEHVRAIDPAALVADQDATPGTGRAVQCGLSVLDAAAHADLARDRAPDEPTHAETSGAVVVVAGDVPLLDGATLGELVATHVADGNAVTVLTTEARDPKGYGRIVRDGDAVVAIVEERDATDEQRAIREINSGIYAFDAAVLRAGLERLGTDNAQGELYLTDVLGIARADGRRVGAFVAGDFSETEGVNDRVQLAELGREMNRRTLERHMRGGVTVVDPASTFVDVTVSFERDVVVHPGTQLLGSTSLAEGCEVGPNSRLTDTSVGAGARVDNSVALSAVVGAEASVGPFAYLRPGTRLGRASKAGTYVEMKNASVGEGTKVPHLSYVGDATIGDHTNIGAASVFVNYDGVNKHHTTVGSHCRTGSDNMFVAPVTLGDGVYTAAGSVITKDVPAGSLAVARGQQRNIEGWVERKRPGTAAAEAARRAQEQGAAGADGAGTDDASTDGATSDDATSDDATSDDATSDDVTSDDVTKGDR",
    "response#2": "AVIMAAGKGTRMKSKLPKVMHSLAGKTLIEHVLDMVDHAGIERSLVIVGHGRENIEACINKRAEMVVQTEQLGTGHAVRQAIPYLDDNQTILVLSGDQPLLRSETIEALIELHQAQKASATVLTTVLDDPTGYGRVLRDASGGVVAIVEHKDASEEQRAITEINSGIYAFDAAVLRRALAEVTTDNAQGELYLTDVLGIARGDGHRVGAHLVSDRWLTEGVNDRVQLARLGAELNRRTLERWMRAGVTIVDPASTFIDADVELAQDVTLLPFTRLLGKTRIEEDAVIGPQTSIENCTVGRGAEVFNSVARDAVIGERCHIGPFSYLRPGTRLEAEVKVGDFVEIKNSWIETGAKVPHLSYIGDTHVGKSANIGAGTITCNYDGTKKHPTKIGDHAFIGSNTNLVAPLEVGDYAVTGAGSTLTKNVPARALAVERSQQRNIEGWVERHRPGSAAARAAERALGADSADSAESADSADSADSADSADSADSADSADSADSEKG",
    "response#3": "LILAAGKGTRMKSALPKVLHPVGGQSMLGRVASTAFQFDNPHVVVVVGHGAEAVQSHLSAQPRIQFATQSEQLGTGHAVQCASSALVDHAVTLILYGDVPLIQFESLQPLVQAAQSPAGGIGLLTVTLDDPTGYGRIVRDAAGAVTGIVEQKDASAAQQSIREINTGILAAPTARLKTWLAGLKNDNAQGEYYLTDIVAMAAADGVAVAASQPSAEAEVLGVNSRSQLAALERAHQLRQAEALMAANGVTVVDPASTWVDVTVTLASDVTLKPGTQLHGSTEVATGAAIGPDSTLTDTTVGEGAVVKRTDATEAVIGAGATVGPFTYLRPGTVLGEEGKIGAFYETKKVTIGRGAKLSHLGYAGDAEIGEYANIGCGNITANYDGVDKHRTVIGSHVRTGSNTVFIAPVTVGDGAYTGAGATVREDVPAGALALNAVDSDATVGENAVIGPYARLRPGARLGNEVHIGNFVEVKGVTLISPENTFIGPDVVIGSDTVIHPGTVLSGNTTIGAGCEIGPNSEINNCQVGDETVIRHSVAADSEIGARVAIGPFAHIRPASEIGDEAKIGNFVEIKKTVFGKGSKASHLSYIGDAEVGSGVNLGCGSITVNYDGKNKFLTKIEDDVFIGCNSNLVAPVTVGKGAYVAAGSTITDDVPGEALSIARARQRTITGYKRPQK"
  },
  {
    "reference": "EAPEPIVESAVPEAVVEPTVEVTFVEEPVVEVTTVVEEVTPETQPEPTPVSEVAPSQPLSWSSIVSQTTEVTTNVTETRFTEEPEDKKPSEEKPKKQKAKKEKKTKVVEAPEPIVESAVPEAVVEPTVEVTFVEEPVVEVTTVVEEVTPETQPEPTPVSEVAPSQPLSWSSIVSQTTEVTTNVTETRFTEEPEDKKPLEEKPKKQKPKKEKKTKVVEAPEPIVESAVPEAVVEPTVEVTFVEEPVVEVTTVVEEVTPETQPEPTPVSEVAPSQPLSWSSIVSQTTEVTTNVTETRFTEEPEDKKPLEEKPKKQKSKKEKKTKVVEAPEPIVESAVPEAVVEPTVEVTFVEEPVVEVTTVVEEVTPETQPEPTPVSEVAPSQPLSWSSIVSQTTEVTTNVTETRFTEEPEDKKPLEEKPKKPKAKKEKKTKVVEAPEPIVESSVPEAVVEPTVEVTFVEEPVVEVTTVVEEVKPETQPEPTSVSEVAPSQPLSWSSIVSQTTEVTTNVTETRFTEEPEDKKPLEEKPKKPKAKKEKKTKVVEAPEPIVESSVPEAVVEPTVEVTFVEEPVVEVTTVVEEVTPETQPEPTPVSEVAPSQPLSWSSIVSQATEVTTNVTETRFTEEPEDKKPLEKKKKKKK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that can perform actin binding, protein kinase binding in nuclear outer membrane, nuclear envelope, cytosol, perinuclear region of cytoplasm, cytoplasm to support somatic muscle development, larval visceral muscle development, nucleus localization, flight, microtubule anchoring.2. The protein should exhibit Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "GNSSPKDMQDLIHLSGPLTEHAVMRTLQARFNERRYFTNVGPILLSINPYLDVGNPLTLTSTRAMPLAPQLQKIVQEAVRQQSETGYPQAIILSGTSGAGKTANAMLMLRQLFAIAGGGPETDAFKHLAAAFTVLRSLGSAKTTTNSESSRIGQFIEVQVTDGALYRTKIHCYFLDQTRVIRPLPKEKNYHIFYQLLAGLSREERQKLHLDGYSPANLRYLRGDIGQNEQEDAARFQAWKTCLGILGIPFLDVVRVLAAVLLLGNVQFIDGGGLEVDVKGETELNSVASLLGVPPAALFRGLTTRTHNVRGQLVKSVCGDGDANMTRDCLAKALYCRTVATIVRRANSLKRLGSTLGTLSSDSNESVHNQADVASQHASTIGGGNAGSKSMAALNNAVRHATSDGFIGILDMFGFEEPSPHAHLEHLCIF",
    "response#2": "GNSSPKDMQDLIHLSGPLTEHAVMRTLQARFNERRYFTNVGPILLSINPYLDVGNPLTLTSTRAMPLAPQLQKIVQEAVRQQSETGYPQAIILSGTSGAGKTANAMLMLRQLFAIAGGGPETDAFKHLAAAFTVLRSLGSAKTTTNSESSRIGQFIEVQVTDGALYRTKIHCYFLDQTRVIRPLPKEKNYHIFYQLLAGLSREERQKLHLDGYSPANLRYLRGDIGQNEQEDAARFQAWKTCLGILGIPFLDVVRVLAAVLLLGNVQFIDGGGLEVDVKGETELNSVASLLGVPPAALFRGLTTRTHNVRGQLVKSVCGDGDANMTRDCLAKALYCRTVATIVRRANSLKRLGSTLGTLSSDSNESVHNQADVASQHASTIGGGNAGSKSMAALNNAVRHATSDGFIGILDMFGFEEPSPHAHLEHLCIV",
    "response#3": "GNSSPKDMQDLIHLSGPLTEHAVMRTLQARFNERRYFTNVGPILLSINPYLDVGNPLTLTSTRAMPLAPQLQKIVQEAVRQQSETGYPQAIILSGTSGAGKTANAMLMLRQLFAIAGGGPETDAFKHLAAAFTVLRSLGSAKTTTNSESSRIGQFIEVQVTDGALYRTKIHCYFLDQTRVIRPLPKEKNYHIFYQLLAGLSREERQKLHLDGYSPANLRYLRGDIGQNEQEDAARFQAWKTCLGILGIPFLDVVRVLAAVLLLGNVQFIDGGGLEVDVKGETELNSVASLLGVPPAALFRGLTTRTHNVRGQLVKSVCGDGDANMTRDCLAKALYCRTVATIVRRANSLKRLGSTLGTLSSDSNESVHNQADVASQHASTIGGGNAGSKSMAALNNAVRHATSDGFIGILDMFGFEEPSPHAHLEHLCIF"
  },
  {
    "reference": "MKLLLLCLGLILVCAQQEENSDVAIRNFDISKISGEWYSIFLASDVKEKIEENGSMRVFVDVIRALDNSSLYAEYQTKVNGECTEFPMVFDKTEEDGVYSLNYDGYNVFRISEFENDEHIILYLVNFDKDRPFQLFEFYAREPDVSPEIKEEFVKIVQKRGIVKENIIDLTKIDRCFQLRGNGVAQA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The intended protein structure should align with strand-helix-strand-helix-strand-strand-strand-strand-strand-strand-helix-helix-strand-helix-helix criteria.2. The protein should have odorant binding and be active in extracellular space. The designed protein sequence is ",
    "response#1": "CEKTGLESGGVAHGGALNAAQVAHLDEDAFKGGLHRPKFDSEGLHKPHTSGGKTYETGFHYLLEAHA",
    "response#2": "PLCKDPYGKEVQSMVENLLVQANVDTTNAFDNFKQPCPQLTKEQVAMCKGFDYGDKTLKLPCGPLPWPAGLPEPGYVPKTNPLHGRWITVSGGQVAFIKEAIKSGMLGAAEAKKIIADTDHHQTGGMYLRINQYGEVCTVDASVAKFARAKRTWKSGHYFYEPLVSGGNLLGVWVLPEEYRKIGFFWEMESGHCFRIERRR",
    "response#3": "PLCKDPYGKEVQSMVENLLVQANVDTTNAFDNFKQPCPQLTKEQVAMCKGFDYGDKTLKLPCGPLPWPAGLPEPGYVPKTNPLHGRWITVSGGQVAFIKEAIKSGMLGAAEAKKIIADTDHHQTGGMYLRINQYGEVCTVDASVAKFARAKRTWKSGHYFYEPLVSGGNLLGVWVLPEEYRKIGFFWEMESGHCFRIERRR"
  },
  {
    "reference": "MEEFHKVRRLPPYVFEQVNRLKASARAAGADIIDLGMGNPDLPTPQSIVDKLCEVVQDPRTHRYSSSKGIPGLRRAQAAYYARRFGVKLNPETQVVATLGSKEGFANMAQAITAPGDVVLCPNPTYPIHAFGFLMAGGVIRSISVEPDESFFPPLERAVRHSIPKPLALILNYPSNPTAQVATLDFYKDVIAFAKKHDIIVLSDLAYSEIYFDDAPPPSVLEVPGATDVTVEFTSMSKTFSMPGWRMGFAVGNERLIAALTRVKSYLDYGAFTPIQVAATQALNGDGSDIAEVRAIYKRRRDVMVESFGKAGFEVPPPPATMFAWAKIPEKFRHLGSLEFSKLLVEKADVAVAPGIGFGEQGDDYVRLALVENEHRIRQAARNIKRFLSSADETMHNVISLNAHR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC",
    "response#2": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC",
    "response#3": "ANVHIIMTSKRGKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTYR"
  },
  {
    "reference": "MELILSTSPAELTLDPACQPKLPLDSTCQPEMTFNPGPTELTLDPEHQPEETPAPSLAELTLEPVHRRPELLDACADLINDQWPRSRTSRLHSLGQSSDAFPLCLMLLSPHPTLEAAPVVVGHARLSRVLNQPQSLLVETVVVARALRGRGFGRRLMEGLEVFARARGFRKLHLTTHDQVHFYTHLGYQLGEPVQGLVFTSRRLPATLLNAFPTAPSPRPPRKAPNLTAQAAPRGPKGPPLPPPPPLPECLTISPPVPSGPPSKSLLETQYQNVRGRPIFWMEKDI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design must be focused on enhancing the efficiency of N-terminal peptidyl-aspartic acid acetylation.2. The protein should be designed to catalyze the following reaction acetyl-CoA + N-terminal L-glutamyl-L-glutamyl-L-glutamyl-   [protein] = CoA + H(+) + N-terminal N-acetyl-L-glutamyl-L-glutamyl-L-   glutamyl-[protein].3. The protein must exhibit the following characteristics: In vitro, shows high activity towards Met-Asp-Glu-Leu and Met-Asp-Asp-Asp. The designed protein sequence is ",
    "response#1": "LLTELFSNKGSGTLFKNAERMLRVRSLDKLDQGRLVDLVNASFGKKLRDDYLASLRPRLHSIYVSEGYNAAAILTMEPVLGGTPYLDKFVVSSSRQGQGSGQMLWECLRRDLQTLFWRSRVTNPINPWYFKHSDGSFSNKQWIFFWFGLADIRDSYELVNHASFWEAKAQLAKSCKVLVDALRHNAAAAVPFFGGGSVLRAAEPAPHASYGGIVSVETDLLQWCLESGSIPILCPIGETAARRSVLLDSLEVTASLAKALRPTKIIFLNNTGGLRDSSHKP",
    "response#2": "ERMLRVRSLDKLDQGRLVDLVNASFGKKLRDDYLASLRPRLHSIYVSEGYNAAAILTMEPVLGGTPYLDKFVVSSSRQGQGSGQMLWECLRRDLQTLFWRSRVTNPINPWYFKHSDGSFSNKQWIFFWFGLADIRDSYELVNHAKGLPDSFLVKRSREQLEMDIEKYTIIDRDGVIIACAALNVYEDEKMAEMACVAVHPDYRSSSRGDVLLAEIRKRAKNLDIEKLFVLTTRTVHWFQERGGRVDPHERMLRVRSLDKLDQGRLVDLVNASFGKKLRDDYLASLRPRLHSIYVSEGYNAAAILTMEPVLGGTPYLDKFVVSSSRQGQGSGQMLWECLRRDLQTLFWRSRVTNPINPWYFKHSDGSFSNKQWIFFWFGLADIRDSYELVNHAKGLPDSFLRVRSLDKLDQGRLVDLVNASFGKKLRDDYLASLRPRLHSIYVSEGYNAAAILTMEPVLGGTPYLDKFVVSSSRQGQGSGQMLWECLRRDLQTLFWRSRVTNPINPWYFKHSDGSFSNKQWIFFWFGLADIRDSYELVNHAKGLPDSFHKEL",
    "response#3": "ERMLRVRSLDKLDQGRLVDLVNASFGKKLRDDYLASLRPRLHSIYVSEGYNAAAILTMEPVLGGTPYLDKFVVSSSRQGQGSGQMLWECLRRDLQTLFWRSRVTNPINPWYFKHSDGSFSNKQWIFFWFGLADIRDSYELVNHAKGLPDSFLVKRSREQLEMDIEKYTIIDRDGVIIACAALNVYEDEKMAEMACVAVHPDYRSSSRGDVLLAEIRKRAKNLDIEKLFVLTTRTVHWFQERGVAFDWSRLVKRSREQLEMDIEKYTIIDRDGVIIACAALNVYEDEKMAEMACVAVHPDYRSSSRGDVLLAEIRKRAKNLDIEKLFVLTTRTVHWFQERGTAFDWSR"
  },
  {
    "reference": "MNQKPHVAVVGATGAVGLEMIKTLEQRSFPVSRLTLLASTRSAGKRLKFRGQEVTVQALTKDSFGGIDLALFSAGGSISREFAPVAARQGCVVIDNSSAFRMESSVPLVIPEINAADVEWHEGIIANPNCTTAITLMALYPLHAAFGCRRIFASSYQAVSGTGAKAIVELQRQVDQAVHGEAVTREVYPHQIAFNVLPQVDSFLPSGYTKEEMKMENEGRKIMHHPKFRASVTCVRVPVYRAHSVAVSAEFEKPVTVEEAREVLAQAPGLQVIDEPAKSRYPLPLTQAGQYDCAVGRLRQDCAMENGLAFWVCGDQLLKGAALNAVQIGEELLSRKLDHAGTPALQP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein with protein dimerization activity functions is required to enable 'de novo' L-methionine biosynthetic process, lysine biosynthetic process via diaminopimelate, diaminopimelate biosynthetic process.2. The designed protein have Semialdehyde dehydrogenase NAD-binding domains with specific sequence motifs that are crucial for its activity.3. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.4. A protein that can modulate the overall flux of metabolites through the L-threonine biosynthesis, Amino-acid biosynthesis pathway, enhancing or suppressing its activity as needed.5. The protein's Acyl-thioester intermediate must be carefully engineered to ensure that the amino acids involved in L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH are properly positioned for optimal catalysis.6. The protein should have a high affinity for NADP(+), phosphate. The designed protein sequence is ",
    "response#1": "MAHVAIVGATGAVGHEFLRVLEERAFPLRSLKLLASERSAGRRLPFRGEDHVVEALADDSFRGIDVAFFSAGGGTAKAYARLASDCGAVVIDNSSAFRLVDGVPLVVPEVNAEAIAGRPRGIIANPNCTTLALVGATGAVGRIMVGLLEQRPDLARKYKFLASARSVGTKITFKGVEHTIELLEPAAFQGVDLVIASTPDEVAAEFLPAAVAAGCKVIDESGYFRMNPDVALVIPEVNPQAALDLRPLVDKAGGLERLFVSSYQAVSGSGIKGVQELVSQTRSGVSQDLAALALDGRAITLPEPEVYVAPIAFDVVPLAGSIVEDGSEETDEEQKLRNESRRILHIPDLRVSGTCVRVPVFTGHTLTIHAEFSGAITADEAREVLASAPGVRLVEVPTPLEAAGRDEVLCGRIRQDQAVEGNRGLVLVLSGDNLRKGAALNTIQIAELVSAELLA",
    "response#2": "VVAVVGATGLVGEILLRILEDRAFPVSELRPMASARSAGSKVAFRGQEWQVLEARADAFAGVDLVFFAATGALSRELAPAAVEAGATVIDKSSTWRLRDGVPLIVPEINADQIPTGRLVTTAAVAAGRRHFVSGICVRVPVFTGHLAPLQRRCGLTRVVVSTYQAASGAGQRAVAELRSATRAVLADEPPPTAAALPQTLAFNVFPHVDVFMADGYTREEDKMLYEVRKILAMPELRVDATCVRVPVERCHSESVAVELERALSPTEARALFAGAPGVVLQDDPHGNDYPMPRAYSGKDPVAVGRVRQGRVFEPGLTFWLVGDQLL",
    "response#3": "MTNVAIVGATGAVGETLLAILAERQFPIGELHLLASERSAGEKLEYGARKLVVRDIAGFDPGGVDIALFAAGSSVSREYAAKFAAAGAVVIDNSSEFRGDADIPLVVAEVNPEALREDIAVVGATGSVGREILEILEERNFPVKNIFCLASERSEGEIINFRDTDIKVIDLKKFNFTDIDLALFSPGATISKKFAPVAASKGCLVIDNTSYFRMEKDIPLIVPEINSSELDSRPRGIIANPNCTTMAAMPVLKVLHDQFGLKRLIVSTYQAVSGSGIEGVRELDEQVRAGVTQDLTALALDGSAVAFPEPSTYVAPIAFDVVAFAGNLVDDGSEETDEEQKLRNESRKILEIPDLAVSGTCVRVPVFTSHGLSINAEFEKPVTPQQARELLAKAPGVELADVPTPLQAAGTDPSYVGRIRQDQSVEGGCGLAMFVVGDNLRKGAALNAVQIAELLVG"
  },
  {
    "reference": "MYTMELRFIRGGVCAVDGVLAAGCREGKYGVGLIINRGSTAAAVFTSNRVRAEPVKLTERVIADGSISAIVANSGNANCFTGREGMDDARRMARKVAESLSMDESEVAVASTGVIGRRMPIDKIEFLIQSAAAQLENSEAASGALAEAIMTTDTFPKEVAVEFELETGEKARIGAVAKGSGMIAPNMATMLSFITTDVDASSSELTEALRVAVDESFNMLIVDGDESTNDMVIISSTRTSGRIDSNFREALVAVCRELARMMARDGEGVTKSFQVDVVNAGTHEDAKMAARAIAGSSLVKTAIFGADPNWGRIVAAAGYSGAEFDPEEISVTLESDSESVVIVDHGDILAFEGTEELETAERVMTSKEIRIIVDLAAGDESATAYGCDLTYDYVRINAEYTT",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Nucleophile active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The protein can be targeted to cytoplasm and perform glutamate N-acetyltransferase activity, methione N-acyltransferase activity. The designed protein sequence is ",
    "response#1": "SCGGR",
    "response#2": "MDT",
    "response#3": "GIIPL"
  },
  {
    "reference": "MPSVFSKVNNDASLRNPEHLITMRKLDLALKDGPALKLKHDIRNNINTVSRRVLETHTTSQDLLDASTSVNIWPLLLLKIITDSNYGTYYNKHNQDTSSRSLNIARQLLTIQTNWMNQHNFDITAPEFKETFKTWCDKSFMSDPLLANLLFLEDLFSSAVEMSGQVSFLLRKKKNRLTPRLISNKTFMIIKQLDLFICWSNGLVFLRHNKQGYIHQRQFLLLIHNKLCDLLSVYLLSKFTENVCFDVTAQKQVIDLIEEMCNLSIRYGNDYFDIIRTLEGITTGEILMSEEDWRNDSLIQSIHQDLLDVNFCYIGSRLQQILTYASTPLKHELSGLSKITGHPIVDEEGTAERAYERANKVKSLNYLAINSSRNLAVESYIRSYILKHRKWPPCIIQPEAGKWLHSAKERNLDPYHPNIQKQYGSGTFEEFTYVDLEPVKELDQLLHYTQYLKDKTVTLEKSKIFRHYINVYDKPRWEETRLLLVYLFHTNEDLGLSKYLQDYLNTAPDMNLALDYLVIKLVPKEKELKRKARLFGCKTYQDRYRGKVQEEAVMSFMSDYVQDQSMTLDDIGLMHRLYSFRHLLKAYKGWKVLYVNFDVSGWCSNFRHETVHPIAHDILDKYYQTGDFFGKTQLAYEHGLFYIPKQDGSGAYHWEGQLGGVEGLNQDTWMMVYIPQMRLALHEFQAHMFVMAYGDDYKAALLIPPDQANRDITTLKNKIIEVVSNVAQTCFAQDMKVFDSYGSECFISFCKNASVRGVELSQGFRKIQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N); After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.2. The protein should have mRNA (guanine-N7-)-methyltransferase activity, GTPase activity, ATP binding and be active in virion component. The designed protein sequence is ",
    "response#1": "GGVVLVTSSLLGFQPEGDKETTVFAADWVRAVSDVYTERFLVLSGAILGRCLSDEHYPQVLDLEFIINWGDTVLRRKGNKGFKLLKAYEALVLGVVQGKSESDFIDPDRFLRNTLNDIIDDDKDLGAYAVLLIQRLQQIDSAHHLIQLFGLHRIWGHPLVDPAKGMEKMIIIGQKDIVRETSRPEVMGVHFKKLMAQGYREKHGVYPNVKGNGELETLIKNNHDWKEVGSLDNDNHWRLLRFDKTFSIPESFNLSMIVADKSVSPTLSELKENILRKKTVMNQELRRGVLRWINHDSIDPREFLEQVNDKKFPDDHKIIGLRSKEREMNPTPRMFALMSHLMRVYVVITESMLSEHILPYFPQITMTDSQLDLTKKTYSTVKNQAAKIRRSGALYDTKTVCMSLDFEKWNGHMRKESTFHVFEALGDLFGMENLYNETYDIFKDSYFYLADGSYVPSMDAQGNFTPEPPYSFTGHKGGQEGLRQKGWTIFTVVCLDWICRKHNCTYKIMGMGDNQVLQLTMYTYRVDASGKATERGREDMRRVLFGLFDDLLDVFSDLGLPLKPLETWISEDLFVYGKYPVLKGVPLSMDLKKIMRIFPFSS",
    "response#2": "GGVVLVTSSLLGFQPEGDKETTVFAADWVRAVSDVYTERFLVLSGAILGRCLSDEHYPQVLDLEFIINWGDTVLRRKGNKGFKLLKAYEALVLGVVQGKSESDFIDPDRFLRNTLNDIIDDDKDLGAYAVLLIQRLQQIDSAHHLIQLFGLHRIWGHPLVDPAKGMEKMIIIGQKDIVRETSRPEVMGVHFKKLMAQGYREKHGVYPNVKGNGELETLIKNNHDWKEVGSLDNDNHWRLLRFDKTFSIPESFNLSMIVADKSVSPTLSELKENILRKKTVMNQELRRGVLRWINHDSIDPREFLEQVNDKKFPDDHKIIGLRSKEREMNPTPRMFALMSHLMRVYVVITESMLSEHILPYFPQITMTDSQLDLTKKTYSTVKNQAAKIRRSGALYDTKTVCMSLDFEKWNGHMRKESTFHVFEALGDLFGMENLYNETYDIFKDSYFYLADGSYVPSMDAQGNFTPEPPYSFTGHKGGQEGLRQKGWTIFTVVCLDWICRKHNCTYKIMGMGDNQVLQLTMYTYRVDASGKATERGREDMRRVLFGLFDDLLDVFSDLGLPLKPLETWISEDLFVYGKYPVLKGVPLSMDLKKIMRIFPFSKH",
    "response#3": "MSTFLTTDLQKFCLNWRQESSNIFAERLNEIYGLPGFFNWMHRRLEKSVLYVADPHCPPYNTENIELDDVENKQIFIKYPMGGIEGYCQKM"
  },
  {
    "reference": "MTEGTLAADEVRVPLGASPPAPAAPVRASPASPGAPGREEQGGSGSGVLAPESPGTECGADLGADEEQPVPYPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRPYYQTEEGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQAEDGGAGRNACINWNQYYNVCRSGEFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIMGSFFMINLCLVVIATQFSETKQRENQLMREQRARYLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKVDPSSTVHGQGPGRRPRRAGRRTASVHHLVYHHHHHHHHHYHFSHGGPRRPSPEPGAGDNRLVRACAPPSPPSPGHGPPDSESVHSIYHADCHVEGPQERARVAHSIATAASLKLASGLGTMNYPTILPSGTVNSKGGTSSRPKGLRGAGAPGAAVHSPLSLGSPRPYEKIQDVVGEQGLGRASSHLSGLSVPCPLPSPQAGTLTCELKSCPYCASALEDPEFEFSGSESGDSDAHGVYEFTQDVRHGDCRDPVQQPHEVGTPGHSNERRRTPLRKASQ",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have Helical; Name=S4 of repeat IV, Helical; Name=S3 of repeat IV, Helical; Name=S6 of repeat I, Helical; Name=S1 of repeat I, Helical; Name=S6 of repeat III, Helical; Name=S1 of repeat IV, Helical; Name=S2 of repeat IV, Helical; Name=S6 of repeat IV, Helical; Name=S4 of repeat I, Helical; Name=S4 of repeat II, Helical; Name=S3 of repeat II, Helical; Name=S5 of repeat I, Helical; Name=S1 of repeat II, Helical; Name=S5 of repeat II, Helical; Name=S5 of repeat IV, Helical; Name=S2 of repeat II, Helical; Name=S3 of repeat III, Helical; Name=S3 of repeat I, Helical; Name=S2 of repeat I, Helical; Name=S4 of repeat III, Helical; Name=S6 of repeat II domains to ensure proper insertion into the membrane.2. The protein should exhibit Basic residues, Basic and acidic residues, Polar residues compositional bias.3. The protein should have a high affinity for Zn(2+). The designed protein sequence is ",
    "response#1": "HSLFSMFIMITILPNCVVMTMSDPPPWPIHVENTFTGINTFESLIKMLARGFCIDDFTFLRDPWNWLDFSVIMMAYLTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPQPFNDTNTTWYGNDTWYSNDTWNSNDTWSSNDMWNSHESMASNYTFDWDAYINDEGNFYFLEGAKDALLCGNSSDAGHCPEGYKCIKTGRNPNYGYTSHDTFSWAFLALFRLMIQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVTMAYAEQNEQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQEM",
    "response#2": "SMFAIVAFGLLVCWSNAVGGLILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASMVVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSYPLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEAEKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAMNVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVGLLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSGLANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGCSLEEISGDV",
    "response#3": "TIPFYLILLLLPLLNFRSASFFARFTFLGTISVIYLIFLVTYKAIQLGFHLEFHWFDSSMFFVPEFRTLFPQLSGVLTLAFFIHNCIITLMKNNKHQENNVRDLSLAYLLVGLTYLYVGVLIFAAFPSPPLSKECIEPNFLDNFPSSDILVFVARTFLLFQMTTVYPLLGYLVRVQLMGQIFGNHYPGFLHVFVLNVFVVGAGVLMARFYPNIGSIIRYSGALCGLALVFVLPSLIHMVSLKRRGELRWTSTLFHGFLILLPGVGLLYTELVQGIPPVFPRLVDKIPSVHAVFVFMSIKHLPIPRVAPAERFIFQRVGPDAGHRIFRCVARYGYTDPLEGAKEFAAFLLDRLKVFVYEEAVFACQCAEDGGGGGGGDDDGVLRRAEEMAAEEKRLIDAEAERGLVYLMGEANVEAAPGSSLMKQIVVNYVYTRLRKNLREEHKALSIPKDQLLKVGITYEL"
  },
  {
    "reference": "MAEAERIIGTPDIPEAAGAEPEQADGESGGAGAGTERPKGRRRIAVLFAVAALAYAFDLVSKLIVVAKLEHHAPIEIIGDWLRFEAIRNAGAAFGFGEAFTVIFTVIAAAVIVVIARLARKLYSLPWAIALGLLLGGALGNLTDRIFRSPGVFEGAVVDFIAPKHFAVFNLADSAIVCGGILIVLLSFRGLDPDGTVHKD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that This protein specifically catalyzes the removal of signal peptides from prolipoproteins.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The protein design should consider the endogenous proteins and enzymes present in the Protein modification, lipoprotein biosynthesis (signal peptide cleavage) pathway to avoid interference with native metabolic processes.4. A key design requirement for the protein is the optimization of the active site to achieve high catalytic efficiency for Release of signal peptides from bacterial membrane   prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in   which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and   Zaa (Gly or Ala) have small, neutral side chains..5. The protein should be optimized for plasma membrane-specific folding and stability, while maintaining aspartic-type endopeptidase activity. The designed protein sequence is ",
    "response#1": "MDFFSSSTFFILLWILLLVVVCRYYNRYLNYWKDRGVPYIENLPMIGNFFPFMLRKRSFHEDCSKLYSKFNGERVVGFYLMRTPVLVVRDPELIKNILVKDFNHFTNRKTTIPCNEPLNKHLFNLEGARWKNLRSKLTPTFTSGKLKLMFETIVDSCQHLVDFLKQEAEKSGCFEMKEVCARFTTDIIGTCAFGLECNSLKNPESEFRRMGRKVFSPSWKIAIKFLSINLAPKLAKLLNIKLFDDRVSDFFIKTVKETIEYREKNGIIRNDFMDLLIELKNSKSSNTDNNHDVQFTKDEIAAQCILFFIAGFETSSSTMSFALYELSQNKDVQEKLRNEINNVLERHNGKITYEALHEMKYLDQVVNETLRLYPPLPFLDRMCTKDYMVPDTDIVIPKGMSVVIPIYAIHHDPEHFPEPDRFDPERFSEENKKNIKPFTYLPFGDGPRSCIGMRFGLLQTKVGLVYLLQNFKFSICEKTEIPLKIDPQGFMLSPKGGIHLKVEKI",
    "response#2": "MNIVRKFYFRKKMEMGKSEAEIIADFRKKY",
    "response#3": "MAQPPLPPVPLAVLALLLLLLLLLLLLPPPPPRPLPRLSALARPALLLLLLLLLPLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLXLPPLPLPPPLLLLLLLPPPPPPLPPPLPPPLLPLLPPPPPLLPPPPPPLPPPLPPPLPPPLPPPLLPPPLPPPLLPPQPPLLPPPLPPPLLPPPLPPPLLPPELPPPLLPPELPPPLPPELPPPLPPPLPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPPELPP"
  },
  {
    "reference": "MAHQSALIAEDIHAYLKSQEEKGLLRLITCGSVDDGKSTLIGRLLWDSKMVFEDQLESLTADSKRIGTQGEKIDYALLLDGLEAEREQGITIDVAYRFFSTDKRKFIVADTPGHEQYTRNMVTGASTAQVAIILIDARKGVLTQTRRHSYLASLVGIRHVVLAINKMDLVDYSEHRFKEIYNEYKTFAQDLGFTEIRPIPISALEGDNVLELSPRTPWHQGPTLMKYLEQVQVTEATDQRPFRLRVQWVNRPDLDFRGFCGTIASGTIRPGDPVMVSGSGQTSRIQRIVTMDGDLPEAVAGQAITLTLEDEIDISRGDTLSDPGNHPFRASRLEARLVWLHEEALLPGRSYLLKAGPSTTTAQISSLRHKVNVNTLQLKPAQTLAHNEIGVCLLRLDKTIAFDSYQENRATGSFILIDRVTNATVGAGMIDTAVEQRENNQWHRISLDRSVRAGRLGQAPKALWFTGHPASEKSTLAELVEKKLHSLGRVTTLLDENQLRQGLNHDLGFTAADQAEHVRRIAETTKLFVDSGLIVLATVTSPFRSERQLAREIIGSEEFIEVHVNTPIAVCEERNNSGLYRRTRTEESRNIAESDCLYEPPIAAEITVDGSSQDLKQLTEEIVNHLLGDPATLEWTI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should consider the endogenous proteins and enzymes present in the Sulfur metabolism, hydrogen sulfide biosynthesis, sulfite from sulfate: step 1/3 pathway to avoid interference with native metabolic processes.2. The designed protein must have at least one Tr-type G domain.3. The protein design must be able to perform adenylylsulfate kinase activity, GTP binding, ATP binding, sulfate adenylyltransferase (ATP) activity in order to contribute to hydrogen sulfide biosynthetic process, sulfate assimilation.4. The protein should have a low Km value for adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate   + ADP + H(+).5. The GTP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "RILTAGSVDDGKSTLIGRLMFDSKMIYEDQLAALERDSKRIGHAGEDIDYALLLDGLKAEREQGITIDVAYRYFSTNKRKFIIADTPGHEQYTRNMVTGASTANLAIILIDATKGVITQTRRHTFLVSLLGIKHVVLAVNKMDLVDFDQKVFDSICEDYKNFVAQLDIPDVTPIPLSALKGDNFDATIVWMAETPLLPGNVFDFKLGSKTVSGRLNTMHNRIDVNTLEKVPAPGLELNEIGLAEISLDETVCFDSYKDNRATGSFIIIDRLSNVTVGAGMFRMPVQLAIRPDASFRGYAGTIAAGRIAPGDPVLLIPSGRRTTIARLLDAGEDCAEAGPGAAVTLTFADEVECGRGELVSADQFEGEVIWMHDEALLPGRSYLIKFGTQSAAAQISELKYKVNVNTLEHAAAKTLDLNEVGICNIVLDRNVAFDPYADSQGTGRFILIDRYSNATVGVGMIRHQLRRSQNIHPTDITRESHEAMKNQTPKVLWFTGLSGAGKSTIANLVEKRLHAMGKRSYIMDGDNVRHGLNRDLGFTDADRVENIRRVGEVARLMMDAGLIVLTAFISPFRAEREMVRRMMPAGEFIEVHVDTPLEVAERRDVKGLYKKARSGELKNFTGIDSPYEAPEHPDIRIDTTAMTPEEAADRIVEYVLGR",
    "response#2": "LTCGSVDDGKSTLIGRLLHDSKMIYEDQLAAIEADSVKSGTTGGKIDLALLVDGLQAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRNMATGASTCDLAIILIDARHGVQVQTRRHSFIASLLGIKHIIVAINKMDLVDFSMSVFDKIKADYVKFSENLDSTNFHFIPMSALTGDNRFITCGSVDDGKSTLIGRLLYESKMIFEDQLTQLEVDSKKVGTQAGDLDFALLVDGLAAEREQGITIDVAYRFFSTDRRKFIVADTPGHEQYTRNMITGASTADVAIILIDARKGVLTQTRRHSYLVSLIGIRHVVLAINKLDMVGYSEEVYNRIDHDYRVFAKKVGLRNIVTIPMSALKGDNIIELSRNTPWYCGDTLMGYLETVQVEKFDASIVWMAEQPLLPGKDYLIKHACSLVPGRISTLRYEIDVNTLHRSPAAELKLNQIGRCQIQLSQPIALDDYRRNKGTGAFIVVDRVSNITVAAGMETADQFEATIVWMDEAEMLPGRPYIMKIGTQQAAVTITEPKYEVNVNTMERLAAKTLRLNSIGVCNISTDRQVTFAPFEDNKTLGSFILIDRVTNATVGAGEVLYTSRETADQFEATIVWMDEAEMLPGRPYIMKIGTQQAAVTITEPKYEVNVNTMERLAAKTLRLNSIGVCNISTDRQVTFAPFEDNKTLGSFILIDRVTNATVGAGEVLYTSR",
    "response#3": "RFITCGSVDDGKSTLIGRLLWDSKLIFEDQLAALETDSKKVGTQGGDIDYALLMDGLQAEREQGITIDVAYRFFSTDKRKFIVADTPGHEQYTRNMATGASNADVAVILVDGRKGILTQTKRHSYIVSLVGIRKIVVAVNKMDLVDYAKDRFVAIEEEYREFAKDLGFDDITFVPISALKGDNIIQPSENTHWYHGPTLMSYLETVPVAHVADQFTANIIWFDEHPLLPGRSYIFRTETDQANATVTELKYRVDVNSFAHQAAKSLDLNEVGVCNLSTQAPIAFDTYAENRTTGAFILIDRITNATVGAGMVADQFEATVVWMIDEPLLPDRPYLMKIGTKTVTANVSGIKYKVNVNTLEQVAATKLELNEIGACNLNTDQSIAFDPYSEDRDTGGFILIDRLSNNTVGAGEVANQFETTLLWMADEPMFPGRPYVMKIGTSTVNATITRPKYRINVNTLGHEAANDLDLNEVGVCNISTDRPIAFDPYSENHDTGAFILIDKITNGTVGMGTIV"
  },
  {
    "reference": "MNSFMTVGLGHYLAVAAVLLVLGIFGIFLNRKNVIVMLMSIELILLAVNLNMVAFSASLGDLAGQVFAMFILTVAAAEAAIGLAILVIYFRNRGSIQVEDVNLMKG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that localizes to plasma membrane and can perform NADH dehydrogenase (quinone) activity with high specificity and efficiency.2. The protein must exhibit the following characteristics: The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. The designed protein sequence is ",
    "response#1": "ANDLISLYLALELQSLALYVVAAINRDSLKSTEAGLKYFVLGALSSGMLLYGMSLVYGFTGHTHFTEIAQALSVEGARSLGLIFGLVFILAGIAFKISAVPFHMWTPDVYEGAPTPVTAFFAAAPKVAAMAMLTRIVITAFQPVLADWQQVVVFISIASMLLGSFAAIGQKNIKRLMAYSSIGHMGYALVGLAAGNQTGVSGVMLYMVIYMIMTLGTFAIIMSMRRKDGTVVEDVNDLAGLSATNPFMAVVLTALMFSLAGIPPLAGFFAKYFVFVAAIEAKLYALAIIGVLASVALLN",
    "response#2": "DVGGMTPVFLAFTDRQKIYDVVEAITGFRMHPAWFRIGGVAHDLPRGWEGLLREFLDWLPKRLDSYVKVALRNSVLRARAEGVAAYGAKEALDWGVTGAALRATGIDFDVRKWRPYSGYENFNFEVPVGDGVSDCYSRVMLKVEEMRQSLRILEQCLNNMPAGPFKADHPLTTPPPKERTLQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSFPHLQQIPSVIRGSLVSDLIVYLGSIDFVMSDVDEM",
    "response#3": "DVGGMTPLLYGFEEREKLMEFYERVCGARLHANYYRPGGVAADLPAGLLEDIAAWCETFPKVLDDIETLATDNRIFKQRVVDIGVVSPEQALDWGFTGPNLRASGIAWDLRKSQPYDVYDQMEFDIPVGKNGDGYDRYLVRVLEMRESVKIMKQCIAKMPGGPVRVEDNKITPPKRSEMKTSMESLIHHFKLFTEGFKVPAGEVYGAIEAPKGEFAVYLVSDGTGKPYRCKIRPPGYVHLQALDMMSKGHMLADVVANIGSIDIVFGEIDA"
  },
  {
    "reference": "MSAGDGLPPLREVVARHGLEPKKALGQNFLYDLNLTGRIARAAGPLAGVTVVEVGPGPGGLTRALLAEGAARVVAIERDPRALPALAEIAAHYPGRLEVVDADALAFDPRPLVGDAPARIVANLPYNVGTALLTGWLDGEAWPPWWDQAVLMFQREVAERIVAGPEERADYGRLGVLCGWRTEAEILFDVSPSAFVPPPKVTSSVVRLVPRAQPLPCRAGALEAVTRAAFGQRRKMLRQSLKALTPAAGDLLAAAGLSETARAEEIPVAGFVDLANRWDAHRKAGAPVGTPA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: May play a critical role in biogenesis of 30S subunits.; Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. The designed protein sequence is ",
    "response#1": "ITRKIVRLSGAKPEENILEIGPGFGALTKEIIEVCPSFTVVEKDPKLAAFIRTEYPQLNVIEADFLKIDLEAVTGARKLRILGNIPYSITSPILFRLLEYRRCFMHATLMMQHEVAMRIVAVPSTKEYGILAVQLQAFFDVSYGFRVGRKVFKPQPGVDSAVITITPKK",
    "response#2": "ITRKIVRLSGAKPEENILEIGPGFGALTKEIIEVCPSFTVVEKDPKLAAFIRTEYPQLNVIEADFLKIDLEAVTGARKLRILGNIPYSITSPILFRLLEYRRCFMHATLMMQHEVAMRIVAVPSTKEYGILAVQLQAFFDVSYGFRVGRKVFKPQPGVDSAVITITPKR",
    "response#3": "ITRKIVRLSGAKPEENILEIGPGFGALTKEIIEVCPSFTVVEKDPKLAAFIRTEYPQLNVIEADFLKIDLEAVTGARKLRILGNIPYSITSPILFRLLEYRRCFMHATLMMQHEVAMRIVAVPSTKEYGILAVQLQAFFDVSYGFRVGRKVFKPQPGVDSAVITITPKR"
  },
  {
    "reference": "MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTHPDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: CRP and Spot 42 coregulate at least 14 operons involved in cellular metabolism, forming a coherent feedforward loop; Spot 42 base pairs with and represses expression from a number of mRNAs encoding proteins involved in the uptake and catabolism of nonpreferred carbon sources; Among the targets of CRP is the spf gene which encodes the dual-function sRNA Spot 42; spf expression is repressed by CRP.2. The designed protein should be involved in positive regulation of DNA-templated transcription, negative regulation of DNA-templated transcription, carbon catabolite repression of transcription, DNA-templated transcription by localizing to protein-DNA complex, cytosol and exhibiting DNA binding, bending.3. The designed protein should be able to compete with endogenous proteins or inhibitors for 3',5'-cyclic AMP binding.4. Incorporation of the H-T-H motif domain in the designed protein should not interfere with its overall stability and folding.5. The designed protein have HTH crp-type domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MDALDEIVKRGRPLKKGEFLFRQGDGFDSVYAVRSGALKTFSLSDSGEEQITGFHLPSELVGLSGMDTESHPVSAQALETTSVCEIPFEKTNLLAKTRKLNTMLQQEASTHVDFKVMADRLSEVMESNTFIVSRRGKLLGIAIKQQIENARIRGFLEERQFPEDYTKKLFNVTETTANIAIDSEHTAFPIDNRDMFETSKTTIVPIIGGGERLGTLVLGRMLEDFNEEDLVLAEYGATVVGMEILREKAHEAEDKARKKAVVQMAINEK",
    "response#2": "MDALDEIVKRGRPLKKGEFLFRQGDGFDSVYAVRSGALKTFSLSDSGEEQITGFHLPSELVGLSGMDTESHPVSAQALETTSVCEIPFELRQARFDTLLTTIRDVLNCDSSALLLFEDQHFKPLAINGLAKEVLGRRFSIEQHPRLEAIARAGDIVRFPSESTLPDPYDGLITNHQGKLHVHSCIGLPLLIDDQLIGAITIDALDPNQFDQLKNQELRFISALLNELEDAPQRNRSLYLDPVNHQVMLDNELLMLSKKEYHLLHELMRCAGTVVRKAVLEQRLFGHGDSVESNSLEVHMHNLRRKIGKDRVITVRGIGYLG",
    "response#3": "MDALDEIVKRGRPLKKGEFLFRQGDGFDSVYAVRSGALKTFSLSDSGEEQITGFHLPSELVGLSGMDTESHPVSAQALETTSVCEIPFELNADEYANAARFFYQARQRDLYGAGGSNAICADVQHKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKIICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNISTNLIQEAFFHKNNAELMLSKKEYHLLHELMRCAGTVVRKAVLEQRLFGHGDSVESNSLEVHMHNLRRKIGKDRVITVRGIGYLSDFYQARQRDLYGAGGSNAICADVQHKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKIICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFSDSDLISSDLYELLESISTKRKMEKHTYLFREGMDAEELYLIQSGLIEIGKLTSDGKDLTLRICQKHDIVGELTLFTEEPRYMLSAKVLEDGEVLVINKNKLEKELIQNGALTFEFMKWMS"
  },
  {
    "reference": "MAETKYIFTTGGVASSLGKGIISSSLAKLLIARGFRVTIQKLDPYINIDPGTLNPYEHGECYVTDDGAETDLDLGHYERFTNEPTSQANNVTTGRIYQAVIDKERRGDYLGETVQVIPHITDEIKHRIQILGNSRKFDFVITEIGGTVGDIESLPYIEAVRQMKWEFGNRCLVIHLTLVPFLASSGELKTKPTQHSVKTLLESGVQPDIIVCRTEKHLNQALRNKIALFCNVTPSAVIESIDVETIYDVPMLMRDEKLDQVVLEKMQVPIRKEPDLIKWEEFLFRLKHPVGEVKIGLVGKYVELKDSYKSILEAFIHAGAANQYKVHVRMIHSESINESSVNKLLADLDGILVAPGFGERGIDGKICSIKYARENNVPFLGICLGMQCAVVEFGRNVLGLKDAHSTEMNASTSYPVIDIMADQKSVSNKGGTMRLGSYPCSIRKDSLAYKIYGIAEIRERHRHRYEFNNAYLEQFEKAGMLPVGINKTDNLVEMIELQGHPWFLAVQFHPEYRSTVANPHPLFVNFVAAAITHLKKRQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The active site of the protein must be engineered to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).2. The protein should be optimized for activity in Pyrimidine metabolism, CTP from UDP: step 2/2 pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The protein should be able to bind UTP ligand in a variety of conditions4. The designed protein have Glutamine amidotransferase domains with specific sequence motifs that are crucial for its activity.5. The protein must exhibit ATP binding, CTP synthase activity. The designed protein sequence is ",
    "response#1": "IDAYISVVEALKSACYHNGVKLDLVWIHSGKLEKGDKKELAKFKKVAGIVVPGGFGSRGVEGKIIAAKYARENNKPYLGLCLGMQIATIEFARHILGTKDANSTEFDSKTSNPVIHILPGHNAEEDMGGTLRLGAYPCILEKDSLSFKAYGVPKISERHRHRYEFNNEYREKLEAGGMRFAGMSPDKSLVEIIELRDHPFFVASQFHPEFKSRPLRPHPLFRDFIDNYC",
    "response#2": "LQDAYKSILESFVHAGAMNEVKVQVVNVHSEFITNENVAEKLQGFDGLLVAPGFGHRGIEGKIIAVKYARENGLPFFGICLGMQMAVIEFGRNMLGLKEAHSTEMDLLTKDPVINMMEEQKKIKMMGGTMRLGTYPCEIVPGTLAHRIYGTDQITERHRHRYEFNNDYLEQYEAAGMVASGKNPGTGLVEIIELPNHPFFIGVQYHPELKSTVEQPAPLFVSFIS",
    "response#3": "KDAYLSVVEALHHAGIHHDVKVNIRSVEAEEIEEHGSEKILSGAHGILVPGGFGSRGIEGKIAAARFARERKIPYLGLCLGMQVAVVEFARHVCSLPGAHSSEMDPGCANPVIHLMEEQMRVEDMGGTMRLGTYPCELVPGTKTASAYGVARVNERHRHRYEFNNDYRKRLEEKGMVIAGVYEEKNLVEIVELQDHPWFVGVQFHPEFRSRPVKPHPVFMGFL"
  },
  {
    "reference": "MANETKYIFTTGGVLSSLGKGLASAAIGALLESRGLTVTLQKLDPYINVDPGTMNPFQHGEVFVTDDGSETDLDLGHYERFTSAKLGQDNNFTTGKIYHSVITKERRGDYLGGTVQVIPHITDEIKNSIKLAANGVDVVIVEIGGTIGDIESLPFLEAIRQFRTDVGKENSIYIHLTLVPFIGTAGELKTKPTQHSVKELRSIGIQPDILLCRTDRYLSKDIKSKIALFCNVGVDAVITAKDVDCIYEVPLIFHNEGLDEKIVELLNIWTRKPVLDEWEELVKTIKAPKHTVTIAIVGKYTDLTESYKSLNEALSHGGIPNNCRVDLSFVDSETVDANTCSDKLLEADGILVPGGFGSRGVAGKICAARYAREQGVPYFGICLGMQIAVIEFARHMAGLDGAHSSEFDPATPHPVIYLMTEWYDDKTGKVEQRDATTDKGGTMRLGAYPCRLASGTKAENAYGTGDISERHRHRYEFNTAYKETLEQHGLIFSGISPSGELVEIVELQDHPWFLGCQFHPEFKSRPRNPHPLFREFIRAAVAYSRKKS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a high specificity for L-glutamine, UTP binding, with minimal off-target effects.2. The designed protein must be able to regulate 'de novo' CTP biosynthetic process.3. The presence of CTP synthase N-terminal domains is critical for the interaction of the designed protein with its intended targets.4. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen5. A protein that is capable of catalyzing reactions within the Pyrimidine metabolism pathway with high efficiency and specificity.6. The active site, Nucleophile; for glutamine hydrolysis active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "MFITGGVVSSLGKGIASASLAAILEARGIKVTLLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVRTPMTQRNNFTTGRVYENVIRKERHGDYLGATVQVIPHITDEIKSRIQKGAEGYDVAMVEIGGTVGDIESLPFLEAIRQMGVEQGHDNVLYIHLTLLPYIPTAGELKTKPTQHSVKELRSIGIQPHILLCRADRHIPAEERRKIALFTNVEERAVISVVDASSIYEVPLALHCEKLDEIVVDKLGLPFRDVDWTSWDDLLQRVHNPKHEITVGIVSKYGIEDVYLSVKEALKHACIASGCRVNIKWIESEDVEKASDPARFFNDVDGILVPGGFGNRGVEGNIKAIQYAREHNIPFLGLCFGFQLAVIEFTRHVAGFPDATSSELGPGTHVIDLLPEQKDVKKMGGTMRLGEHMVWVKDNTLAHRLYGKGKISERHRHRYEVNPDLIDHIEHHGLIFSAKNDNRMEMCELPGHKFFLGTQFHPEFKSRPERPAPPFLGFVAAAVERKHAS",
    "response#2": "MFITGGVVSSLGKGIASASLAAILEARGIKVTLLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVRTPMTQRNNFTTGRVYENVIRKERHGDYLGATVQVIPHITDEIKSRIQKGAEGYDVAMVEIGGTVGDIESLPFLEAIRQMGVEQGHDNVLYIHLTLLPYIPTAGELKTKPTQHSVKELRSIGIQPHILLCRADRHIPAEERRKIALFTNVEERAVISVVDASSIYEVPLALHCEKLDEIVVDKLGLSAGPVDLTEWNELVARIKRPSETVEIGLIGKYVELQDSYKSILESFIHAGAANQVKVNVRFIHSEHLNMGNIEKKLAGLNGILVAPGFGERGIEGKIKAVRYTRENKIPFLGICLGMQMAVIEYSRTVLGLSKANSTEMNEKTTAPVINLMEEQKSVTNKGGTMRLGAWDCTLKKGSLASKTYQGASDISERHRHRYEFNNDYKEQLEDAGMLATGINKETGLVEIVEVPEHPWFVGVQYHPEYKSTVANPHPLFVGFIKAARDFQK",
    "response#3": "MFITGGVVSSLGKGIASASLAAILEARGIKVTLLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVRTPMTQRNNFTTGRVYENVIRKERHGDYLGATVQVIPHITDEIKSRIQKGAEGYDVAMVEIGGTVGDIESLPFLEAIRQMGVEQGHDNVLYIHLTLLPYIPTAGELKTKPTQHSVKELRSIGIQPHILLCRADRHIPAEERRKIALFTNVEERAVISVVDASSIYEVPLALHCEKLDEIVVDKLGLQAGKPDLSHWTAMVERIDSYKSLNEALHHAGFTHLLKVSINWVEAEALETQDGDKLLAGADGILVPGGFGDRGSRGMVRAAQIAREQGIPYFGICYGFQWAVVEFARNVCKLEGADSTECDEHTPHKVIYKLRDLLDVDDLGGTMRLGSYPCNVEAGTRTYKAYGRQEIAERHRHRYEFNREYESVLTDKGLVISGRSPDGKFVEAIELPEHPWFVAVQFHPEFQSKPRNPHPLFDHFVTEAYKSLSEALIHAGIHTRTRVNIAYVDSEQIETEGVSCLEGMGAILVPGGFGKRGVEGKITAVRFARENRIPYLGICLGMQVAVIEFARHVAGLENAHSTEFNPKTPYPVIGLITEWTTSQGSREVRSEGADLGGTMRLGGQPCHLKKDSLVRSIYGRDQIVERHRHRYEFNNHYLTAIEDAGMLIAGRSIDGNLVEIVELPDHPWFIGCQFHPEFTSTPRDGHPLFTRFITEAYKSLSEALIHAGIHTRTRVNIAYVDSEQIETEGVSCLEGMGAILVPGGFGKRGVEGKITAVRFARENRIPYLGICLGMQVAVIEFARHVAGLENAHSTEFNPKTPYPVIGLITEWTTSQGSREVRSEGADLGGTMRLGGQPCHLKKDSLVRSIYGRDQIVERHRHRYEFNNHYLTAIEDAGMLIAGRSIDGNLVEIVELPDHPWFIGCQFHPEFTSTPRDGHPLFTRFI"
  },
  {
    "reference": "MAKLYFYYAAMNAGKSTVLLQSSYNYRERGMQTLLFTPAIDTRFQYGTICSRIGLSEQAYAFNNSDNLYVLTQEFQLQTQKYSCVLIDEAQFLTREQVYQLTEITDQMSIPVLAYGLRTDFRGELFPGSQFLLAWADELIELKTICHCGRKAIMNMRIDENGQAVIEGEQVLIGGNESYVATCRLHYKRGEAGKTFPRNKLFNKDTNTF",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be able to bind Zn(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "LPTTTIGSFPQVAGVRSARRSFKQGRISEEQYRAEMQRYIREAIAQQEALGLDVLVHGEPERNDMVEYFGEQLAGFCFTENGWVQGYGSRCVKPPIIYGDVERLKPMTMEWTTYAQQQTNRPLKGMLTGPVTMLCWSFVRDDQPRSATCKQIALAIRDEVQDLEQAGIKIIQVDEAALREGLPLRKSAWREYLGWAVDAFRLTASGVADSTQIHTHMCYSEFNHIAEWIAKMDADVISIEASRSNMELLGVLESFTYPNDIGPGVYDIHSPRVPSVAEMVELLRKASEVIPLERLWVNPDCGLKTRAWPETLASLRNMVAAARY",
    "response#2": "PTTTIGSFPQVAGVRSARRSFKQGRISEEQYRAEMQRYIREAIAQQEALGLDVLVHGEPERNDMVEYFGEQLAGFCFTENGWVQGYGSRCVKPPIIYGDVERLKPMTMEWTTYAQQQTNRPLKGMLTGPVTMLCWSFVRDDQPRSATCKQIALAIRDEVQDLEQAGIKIIQVDEAALREGLPLRKSAWREYLGWAVDAFRLTASGVADSTQIHTHMCYSEFNHIAEWIAKMDADVISIEASRSNMELLGVLESFTYPNDIGPGVYDIHSPRVPSVAEMVELLRKASEVIPLERLWVNPDCGLKTRAWPETLASLRNMVAAAG",
    "response#3": "RNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPSSPATKNPCNIHYSPNDEDKGMVLPGTKCADGKACSNGRCVDC"
  },
  {
    "reference": "MALNLDHSSAVRPAGTSRPSLIGLSKPQLADALSGAGIASDKEARMRANQLWNWLYVNGVTDFERMTNVAKPVRQKLADTFILDRPEIVSEQVSSDGTRKWLFRFRDPANPNYPPVEVETVYIPEEDRGTLCVSSQVGCTLTCTFCHTGTQKLVRNLTAGEVLGQVLMARERLGDFPGGVRPNDGGLVPHGDTRAITNIVMMGMGEPLYNYENVKQALLIASAGDGMSLSKRRITLSTSGVVPFIEPTGREIDVMLAISLHAVNNDLRDVLVPINKKWPIEELLEACRNYPGLSNARRITFEYVMLDGINDSDADARELVRLLAGIPAKINLIPFNPWPGTNYGCSPSSRIERFADIVNKAGYASPVRTPRGRDIYAACGQLKSESERLSKKDREALAG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein with the reaction adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine, the Proton acceptor, S-methylcysteine intermediate must be optimized to ensure efficient catalysis.2. The designed protein should be involved in rRNA base methylation by localizing to cytoplasm and exhibiting 4 iron, 4 sulfur cluster binding, tRNA binding, metal ion binding, rRNA (adenine-C2-)-methyltransferase activity, rRNA binding.3. The S-adenosyl-L-methionine, [4Fe-4S] cluster binding site should be stable and able to withstand changes in environmental conditions.4. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs; m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.5. The designed protein have Radical SAM core domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "GYKRATICVSSQVGCAVNCQFCLTAKLGMRRNLTAGEIAGQVTAVLNRQGIVIGKDRINLVFMGMGEPFLNYDSFMDAVRLLVNEMGIPASRMTVSTSGIEPAIRRFALEPIRPNLALSLNASNDVVRESIMPITRKWDIEALLDAVKSVPLRRQEYVTFEYVLLGGVNDQPEHAREVLALLKGMQAKVNLIVWNPGPGVAYEQPSQEAADAFHKMLVVGGVPAFTRRPRGEDIAAACGQLAGKVTDRSRLYRRRSADQPLPA",
    "response#2": "QNGRGTLCVSSQAGCALDCSFCSTGKQGFNSDLSAAEIVGQVWVANKSFGTIPAKMDRAITNVVMMGMGEPLLNFDNVVAAMSIMMDDLGYGISKRKVTLSTAGVAPMVDKLGEVIDVSLALSLHAPNDALRDQLVPINKKYPLAVILDACKRYITGLGEKRSLTVEYTLLAGVNDQPEHAAELVQLLRDVPCKVNLIPFNPFPHSGYERPSNNAIRRFQDALHHAGYNVTVRTPRGDDILAAVRRTAVA",
    "response#3": "DKRPLIHGLSKSEIGQIVASLGQPSYRATQIWEWLYKRMAFEWNAMSNLPLALREHLDRELAICPGTLTARSADASGSEKLLLTLRDGECVEQVLIPAPGRKTVCVSSQVGCRFKCAFCASGQAGFRRNLEAGEIVAQTLWAALIWERRPSNVVFMGIGEPLDNYDNVLKAIRIINDSEGLGIGARKITISTSGIIPGMERLAGEGLQIELSVSLHAPTQELRSRLMPVARLYPLDRLIRACRSYFEKTKRLITFEYVLIRGTNDQASHARKLAELLSDFPCRVNLLLLSPVPEFKGTSSSPDAANAFIKTLSRAGINATLRAPRGLSVNAACGQLRSSAVA"
  },
  {
    "reference": "MLSGKRIILGVTGGIAAYKSAYLLREFQKAGAEIRVTMTPSATRFVGSETFSALSRNPVAVDVFSGDQPDDDWTKHIHWGEWADLFIIAPCTANTLSKIVHGGSDNMLTATVLAARCPILICPTMDGEMYESPAVSDNLKKAAQMGYHILEPETGYLASGLEAKGRLPELSEILHQADEIIEKNRAKGPLSGKKVLVTAGPTREYIDPVRFISNPSSGKMGVAMADAAVRLGGEVRLLKGPVHIKPDERMNTESFMSAADLFEKVKHHADADVIIMAAAVSDFTPAERSDQKVKKESASDTISLNKTTDILQWLGERKPGGQTLIGFAMETENLKQNAQKKLQDKNSDWICANDLGGKDAGFESDSNTIHLIGKESQKTFQGTKKQIALQILREIFQKP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity to facilitate coenzyme A biosynthetic process.2. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.3. The presence of Mg(2+) is necessary for the protein to function in the desired environment.4. The presence of DNA/pantothenate metabolism flavoprotein C-terminal domains is critical for the interaction of the designed protein with its intended targets.5. The CTP binding site should be stable and able to withstand changes in environmental conditions.6. The designed protein should interact with enzymes and substrates within the CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis pathway to enhance or regulate its activity.7. The protein have enhanced H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2. The designed protein sequence is ",
    "response#1": "KRILLIIGAGIAAYKSLDLIRRLKERGASVTPVLTGAAEEFVTPLSVSALAGEKVYRDLFDLTDEAEMGHIQLSRSADLIVVAPATADMMGKMAAGLANDLASTLLMATDTPVLCAPAMNVRMWDHPSTQRNLKQLQADGIRFVGPNEGDMACGEYGPGRMSEPLEIVAAVEAATAVARAEGLTGKQILVSAGGTREHFDPVRFIGNPSTGKMGIAIAQAALHRGAEVTLVHAPIDSHLLAPLRSIQCIPVTTSQDMQQAMKQNFSSADYIVMSAAVGDVKPNFYSDAKLPKSQLPETLDLVPIPDILAELSTLKRPNQHLIGFAAQTGDFVTPALEKLHRKNLDVIVANPIDQPNSGFGSETNQAVFACGDGTAPVVDGVHVVVSAGGTREPIDAVRVIANRSSGKQGYAVAAEAAARGASVTIVSTVDRPAPVGAAVERVETAAQMQSAMERLAPSAGVVVMAAAVADFRPAAAVDRKIKKHDGVPEIVLEPTPDILAGLGANKPAGQVLVGFAAETDDMAANAQRKLVAKHLDLIVANDVAADATGFQHDTNAVTLLTRDGDEALAGRTVVVTAGGTREPLDPVRYLGNRSSGKQGVALAREAARRGARVRLIAAHVDGDMRDAAAVLTGVTVEDVSTADELARAVTAAASEADIVIMAAAVADYRPETVAEGKLKKDAVGESLTLTLVQTPDILAGLAAARRPGQVVVGFAAETAADRDELLARGRAKAARKGADLLVLNAVGWQHGFGADNTTVVV",
    "response#2": "KRIVLGVTGGIAAYKAVELSRRLVDAGAHVVPVMTKGAERFLGPTTLSALASETVKTRLWDDPETPIPHTKLGQGADLILVAPATARLIGAYRSGLSTDLLTNVLLATRAPVMLCPAMHTEMWEHPAVVDNIATLRSWGVHIVDPESGRLAGGDSGAGRLAAPETIVAAVEAVLAVLAPVRRLAGRTVVVTAGGTREPLDPVRYLGNRSSGKQGVALAREAARRGARVRLIAAHVDGDMRDAAAVLTGVTVEDVSTADELARAVTAAASEADIVIMAAAVADYRPETVAEGKLKKDAVGESLTLTLVQTPDILAGLAAARRPGQVVVGFAAETAADRDELLARGRAKAARKGADLLVLNAVGWQHGFGADNTTVVVLDRGGTVEAVAGSKLDVAHRILDVVAARL",
    "response#3": "KKILVGVSAGIAAYKTPFLVRLFVKAGAEVKVVMTPSSKDFVTPLTLSTVSKNEVFSAFTIEENDNAIWNNHVELALWADIFVIAPATANTISKMANGICDNLLLATFLSAKCPVYYAPAMDLDMYKHKSTQENFKKLASFETIQIPAGFGELASGLIGEGRMAEPEEMVAFAETLLRQKALLRNKKVLVTAGPTYEAIDPVRFIGNHSSGKMGFEIAQAAANLGAEVILVSGPTHYTTSNKQIKYISVISAQQMYDACHQYFEEIDIAILSAAVADYRPKIVATQKIKKATATLHIELEPTKDILASLGAIKKNQILVGFALETENEVENAKNKLKKKNLNLIVLNSLNDKGAGFKTETNKVTLIDKELNKTDFELKTKNEVAKDILNEIVKRLK"
  },
  {
    "reference": "MKLSALLAGLEYTLTGPDVDLSDFQYDSRRIQPGEGFICLVGTATDGHRYIRQAEQAGAAALIVEHPVESHLPQAVVADTRKAMSVMAEHYYGDPADGISMIGVTGTNGKTTTTYMIKAILTRENQKTGLIGTVAHLAGEENLGASMTTPEPKELQGLFRRMADRRCENIVMEVSSHALSQGRVYGVEFDVAVFSNLTQDHLDYHGDMETYASEKKKLFRQCRHAVINRDDPYGAYMAKGLDVPVTYYGIGEPCDVYATDVITHADGVEYTLHVGEKTRHIRVGIPGRFTVYNSLAAIGACLCHGLGLDEVAEGASAIQGVPGRAEVVPTPGTGYTVLLDYSHTPDSLKNILETAREFTRGRIITVFGCGGDRDPQKRPMMGRVAGELSDYCVVTSDNPRTEDPMAIIRAILPGVEETGCPHAVVENRREAIRHAMICAKQDDVILLAGKGHEPYQEIGHVRHPFDEKIVVAELLAELRS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein's functional design necessitates the inclusion of a Meso-diaminopimelate recognition motif motif.2. The protein design must be focused on enhancing the efficiency of peptidoglycan biosynthetic process.3. The presence of Mur ligase C-terminal, Mur ligase central domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "GRMXLHRXNKKNXCFVDFAHTPEALSSSLXELKYXXKGXXWCIFGCGGERDSXKRPIMGAXAEXXSXEVIITNDNPRNENEDKIIKEILSGXKDPXXAQIIKDXKKAIDKCLTQILGSGEENIXLIAGKR",
    "response#2": "VTGTNGKTSVTQLIAQACDRLGQRCGIVGTLGTGFWGALEAGRHTTPDPFAVQAALAALKEQGAQAVAMEVSSHGLEQGRVAAVQFDVAVFTNLSRDHLDYHGTMEAYAAAKAKLFQWPSLRARVINLDDEQGCRLAEVEHASRLITFSLENPAATLYCKHIAFSDAGIEASLVTPQGEARLVSSLIGRFNLSNLLAAIGIGLHLGLDLQAVVAAVTGISLTTASTREGDLFLAQPGARTHGARFVADAVAAGAAAVLTDAEGAAIIAETPGLDQLARIVVDEHPRRFLGGLAAWFEGAGE",
    "response#3": "ITGTSGKTTTAYLTEAALRAAGRVVGLVGTVEIRIDGERVPSLLTTPEAPDLQRLLAVMLERGVDTVVMEVSSHALSLGRVAGSRFAVGGFTNLSQDHLDFHPTMDEYFAAKAQLFDGASPVRAASAVICIDDVWGEQMATIARSSAEQVVTVSTAGGTDWSVAAEVEVSEAGSQTVVVRSPDGAEHVMVVPLPGAYNVANALLAVALAVELGIPFD"
  },
  {
    "reference": "MNMGKNLALWVIIAILLVALFNLFQSPSGRSGQSTLAFSDFLAEVDSGQISEVTIQGNTLTAQTRDNRTVSVYTPDYPSLVERLNDKGVRIIAQPEESLSPLMSALISWFPMLLIIGVWIFFMRQMQGGGGKAMGFGKSKAKMLTEKSGRVTFEDVAGIEEAKGELEEVVDFLRDPQKFQRLGGKIPKGMLLVGPPGTGKTLLARAIAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLNQLLVEMDGFEANEGVILVAATNRPDVLDPALLRPGRFDRQVVVPNPDLEGRERILGVHARKVPLGPDVDLRTVARGTPGFSGADLANLVNEAALLAARLGKRVVTMADFENAKDKVMMGAERRSMIMTDDEKKLTAYHEGGHALVALHTPASDPIHKATIIPRGRALGMVMRLPERDQVSKSYEQLISDLAVAMGGRVAEEIIFGKDKVTTGASSDINMVTQYARKMVTEWGFSDKLGNVKYVDNQEEVFLGHSVAQHKNVSEKTAQLIDEEVRRYSDEAYVFAKRVLTEHLDDLHVLAKGLLEYETLSGKEIDALLRGEEINRAGHSNGGGKDAGGGRRSTVPTTGGARKENPGEGGGDLSPEPQPGS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must have at least one AAA+ ATPase domain.2. The designed protein should have a binding site that is specific to the target Zn(2+) ligand.3. The designed protein should have ATP binding, ATP hydrolysis activity, metalloendopeptidase activity within plasma membrane to regulate protein catabolic process.4. The protein must exhibit the following characteristics: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins; Plays a role in the quality control of integral membrane proteins..5. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.6. The protein must bind to Zn(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "IPRGVLLVGPPGTGKTLLAKAIAGEAGVPFFSMSGSEFVEMFVGVGASRVRDLFRKAKESAPCIVFIDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNSGVIIIAATNRPDVLDSALMRPGRFDRQITVDLPGPAEQLAILRAIASREGLLLDDSALEYIVEHHQGNVRELEGALKRVVAFSRFNNQPITIELAREALADVRPRQQMVMTEDEKKLTAYHEAGHAIVGLNVPQHDPIHKATIIPRGRALGLVLSLPERDQLSVTYTKYKSKIAMAMGGKVAEELIFGKENVTSGASSDIQQVSKIARAMVTQFGFSEELGNIDYANEQQSYLGNYQGQTAISPETQQKIDAEVRRIVDEGYERAKQILTDKNDDLHRLAQGLLEYETLTGSEITKIIESDASLRRDVEALLKKLN",
    "response#2": "VLMVGQPGTGKTLLAKAIAGEAKVPFFSISGSDFVEMFVGVGASRVRDMFEQAKKQSPCIIFIDEIDAVGRHRGAGMGGGHDEREQTLNQLLVEMDGFEGNEGVIVIAATNRPDVLDPALLRPGRFDRQVVVTLP",
    "response#3": "IPRGVLLVGPPGTGKTLLAKAIAGEAGVPFFSMSGSEFVEMFVGVGASRVRDLFRKAKESAPCIVFIDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNSGVIIIAATNRPDVLDSALMRPGRFDRQITVDLPGPADREAILKIHARGERLELPEDVSMERLAAGSVGLTGADIRNLINEAALWATRQDKTEVHMIDFA"
  },
  {
    "reference": "MNACILLTTLVTMITIEKVQGQSYHFSNGWNPGKRSMQEPVCHFRQDVQTLVLKLIEDEVYRMLSDPSCIGGVPTLRNFLKKDLAYTVPLDDKK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in extracellular region that is critical for neuropeptide signaling pathway.2. For general function, the protein need meet that Neuropeptide involved in reproduction3. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "MLLLPW",
    "response#2": "LIPL",
    "response#3": "MERSGTRR"
  },
  {
    "reference": "MAKEKIVLAYSGGLDTSVAIQWLVESGYEVIACCLDVGEGKNLDFIKEKAITVGASESYTIDAKEEFAEDFALIALQAHAYYEGKYPLISALSRPLIAKKLVEVARQEGASAIAHGCTGKGNDQVRFEVAIHALAPDLKVVSPVRDWKWSREEEINYAKEHDIPVPIDLDNPFSIDQNLWGRSNECGVLENPWTTPPEAAYDLTVSLEDAPDTADIVEITFDAGIPISLNGENMSLANLILTLNEIAGKHGVGRIDHIENRLVGIKSREVYECPAAVTLITAHKELEDLTFVREVAHFKPIIEQKISETIYNGLWFSPLTEALVAFLKSTQKFVNGTIRVKLFKGHAIVEGRKSPNSLYDENLATYTSSDTFDQDAAVGFIKLWGLPTKVNAEVNSKVTITTEV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The ATP, L-citrulline binding site should be stable and able to withstand changes in environmental conditions.2. Ideal protein should have a binding affinity for key enzymes in the Amino-acid biosynthesis, L-arginine biosynthesis, L-arginine from L-ornithine and carbamoyl phosphate: step 2/3 pathway to enhance the overall efficiency of the pathway.3. A protein that contributes to urea cycle, arginine biosynthetic process by functioning in cytoplasm with ATP binding. The designed protein sequence is ",
    "response#1": "YSIDQNLWGRAVETGFLEDIWNPPIEDLYAYTADPAEPRDADEVVITFDAGNPVAIDGETVTPYQAIVELNRRAGAQGVGRLDMVEDRLVGIKSREVYEAPGAIALIAAHQELEAVTVERDLARFKRGVDQRWGELVYDGLWFSPLRAALDAFVNDAQQHVSGDVRLTLHGGRATVTGRRSEASLYDFGLATYDTGDTFDQSLAKGFVQLWGLPSKL",
    "response#2": "SHDENLVHITTEGDYLESVANAFDWRDANWITPPTQAPDAIETITLGFRRGVPVSVDGAALGPVELVERLNEAGARNGVGFQDIIENRINGLKVRGVFENPALTILHAAHRKLEKITLGRDVERLRNLVSDDYGDIVYRGLWFSDERLCLQALIDESQKYVSGDVKVQLYKGSCTPCAVESEQSLYSRELVTLHAGRAISGEDATGFLNTLGLRR",
    "response#3": "YSIDQNLWGRAVETGFLEDIWNPPIEDLYAYTADPAEPRDADEVVITFDAGNPVAIDGETVTPYQAIVELNRRAGAQGVGRLDMVEDRLVGIKSREVYEAPGAIALIAAHQELEAVTVERDLARFKRGVDQRWGELVYDGLWFSPLRAALDAFVNDAQQHVSGDVRLTLHGGRATVTGRRSEASLYDFGLATYDTGDTFDQSLAKGFVQLWGLPSKA"
  },
  {
    "reference": "MVEVEELEPLSPMDDEEEEISYDDLKRRMWKDRNLMEKKLKQQKRHSNDVVSFTTHRAEASRRKKMARSQDSVLKYMMKIMEVCKAKGFVYGIVPEKGKPITGSSDSLRRWWKENVQFDQNAPDAITDYLALAAAAAAAELIDKSSSSSSLLHMLQELQDTTLGSLLSALMQHCMPPQRRFPLEKGIAPPWWPTGTELWWGEQGAAHEHGAPPYRKPHDLRKSWKVSVLAAVIKHMSPNLGRVRRLARQSKSLQDKMMAKETDTWSRVLNQEEALLNIKDLKISEDQDDQESSGSKRKSESMEPSKSVYTCQNSSCPKSDVSFGFGDKNSRTGHEIQCLYGSNQEPSQSGEYTSSLLETVPSIVTNSTSEDDYYNVSSRALDKRDDDDHSINGNWMEYFWLEKMQQEFHCSRRFEDDEGTGTDFDQLTESDRSDNVNLNQLTKSDRSDNVNRSAFSVWDMGCEDKDIYMFD",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.2. For general function, the protein need meet that Putative transcription factor that may be involved in the ethylene response pathway.3. The designed protein should be involved in regulation of DNA-templated transcription, ethylene-activated signaling pathway by localizing to nucleus and exhibiting DNA-binding transcription factor activity. The designed protein sequence is ",
    "response#1": "LFQKELTPSDVGKLNRLVIPKKYAVKYMPFISDDQSEKETSEGVEDVEVVFYDRAMRQWKFRYCYWRSSQSFVFTRGWNGFVKEKNLKEKDIIVFK",
    "response#2": "FQKELTPSDVGKLNRLVIPKKYAVKYMPFISDDQSEKETSEGVEDVEVVFYDRAMRQWKFRYCYWRSSQSFVFTRGWNGFVKEKNLKEKDIIVFYTCDVPNNVKTLEGC",
    "response#3": "LFEKAVTPSDVGKLNRLVIPKQHAEKHFPLPSPSPAVTKGVLINFEDVNGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKNLRAGDVVTFERS"
  },
  {
    "reference": "MKYCVLLIMIHLLLVELPQFPEALQGNVYAAAPVVAATDLRLRIRSSYIGRSCKEIRDRYNQHEDGLYYLSTASGTVYQTFCDMTTAGGGWTLVASVHENNVYGKCTMGDRWSSQQGSNPNWPDGDGNWANRATFGTAEGATSDDFKNPGYYDIVAEDISVWHVPNNSPMEHWNLGAILRYHTERSFLSIQGGNLHQLFKLYPVRYNAEASGNTGPVIPIVYDFGDKETTRELYGPNTRNQFEPGFITFRPINNELAAMAICSGVKPTTGGDTEHYCIGGGGHFPEGAPRQCGDFPAFDWNGYGTNTEWSASKQLTEAAVLLFYR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must be able to mannose binding, chitin binding.2. For general function, the protein need meet that In vitro binds N- acetylglucosamine residues.; May specifically recognize carbohydrate chains of pathogens and bacterial components in a calcium-dependent manner The designed protein sequence is ",
    "response#1": "AQNVVYWGQNGGGTIENNDLAAYCQPNSGIDVLVLAFLYQFGNGGNIPSGTIGQSCYISTSGQGQNCEALTAAIHTCQSAGVKIILSLGGATSSYSLQTQAQAEQIGQYLWDSYGNSGNKTVQRPFGSNFVNGFDFDIEVNGGSSQYYQYMIAKLRSNFASDKSNTYLITGAPQCPIPEPNMGVIISNSVFDHLYVQFYNNNNYTVPCALGINGNAPFNYNNWTSFIADTPSAGAKIFIGVPASPLASTGTPSGAQYYAAPDQLAAIVGEYRSDAHFGGIMMWSAGFSDANVNNGCTYAQQAKCE",
    "response#2": "AQNVVYWGQNGGGTIENNDLAAYCQPNSGIDVLVLAFLYQFGNGGNIPSGTIGQSCYISTSGQGQNCEALTAAIHTCQSAGVKIILSLGGATSSYSLQTQAQAEQIGQYLWDSYGNSGNKTVQRPFGSNFVNGFDFDIEVNGGSSQYYQYMIAKLRSNFASDKSNTYLITGAPQCPIPEPNMGVIISNSVFDHLYVQFYNNNNYTVPCALGINGNAPFNYNNWTSFIADTPSAGAKIFIGVPASPLASTGTPSGAQYYAAPDQLAAIVGEYRSDAHFGGIMMWSAGFSDANVNNGCTYAQQAKC",
    "response#3": "MAAPSPAGGAPSHAQSSLPSLPAHLQSDTHLTAHLASRFHVGLPTARLSSQALISLNTYTTSTKGPDGGKEGSAMGEAEDLAKRAFTRLGARGENQAVVFLGESGSGKTTIRSHLLSAFLSFSSTPLSSKLSYAAFLFDTLTTTKSLTTQTASKAGLFLELQYDGSSSVNPTLIGGKIIDHRLERSRITSVPTGERSFHVLYYLLAGTSPAEKAHLGFDKAVHVSTSSGAIGHKRWRYLGHPTQLKVGVNDVEGFQHFKTALRKLEFPRSEIAEICQILATILHIGQLEFASGQATTTHAEESGGYSHEGGETVTIVKNKDVLSIIAAFLGLSVEDLENSFGYRTKTIHRERVTVMLDPKGARQNADELARTIYSLLVAYVIEAVNQRICAAEDSVANTISIVDFPGFAQACATGSTLDQLFNNAATELLYNFCLQSFFDRKADELEREEVSVPATSYFDNTDAVRGLLKHGNGLLSILDDQTRRGRTDNQLLESLRRRFENKNPTIIVEGSKRTSLISQNARSAFTVKHFAGEIDYSVNGLIEENGEFISGDLMRLMKSTKSDFVRELFGQAALQTVTHPKEKTAIMQAQVSSKPLRMPSMARRKTSPSSRLAFDAGDADEVESQAESIAKDSSSGRRKSAMLTSGIQGAAGQFLSSLDIVNKCLSSTNLNPYFIFCLKPNDRRIANQFDSKCVRAQVQMFGIAEISQRLRNADFSVFLPFAEFLGLAEIGNIVVGSDKEKAEVVLDEKRWPGNEARVGSTGVFLS"
  },
  {
    "reference": "MKQVLTELQEKDLQHVWHPCSQMKDYEAFPPIVIKKGEGVWLYDEQNQRYLDAVSSWWVNLFGHANPRISQALSEQAFTLEHTIFANFSHEPAIKLAQKLVALTPQSLQKVFFADNGSSAIEVALKMSFQYHMQTGKTQKKRFLALTDAYHGETLGALSVGGVDLYNEVYQPLLLDTVRAQGPDCFRCPFKHHPDSCHAQCISFVEDQLRMHHKEITAVIIEPLIQAAAGMKMYPAIYLRRLRELCTQYDVHLIADEIAVGFGRTGTLFACEQANISPDFMCLSKGLTGGYLPLSVVMTTNDVYQAFYDDYATMKAFLHSHSYTGNTLACRVALEVLAIFEEEQYIDVVQDKGERMRKLALEAFSDLPFVGEYRQVGFVGAIELVANRDTKEPLPSEERIGYQIYKRALAKGLLIRPLGNVLYFMPPYIITDDEMQFMIQTTKDTIVQFFEEREG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The desired protein should be able to perform (8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine =   (7R,8S)-7,8-diammoniononanoate + S-adenosyl-4-methylsulfanyl-2-   oxobutanoate2. The designed protein should be able to compete with endogenous proteins or inhibitors for pyridoxal 5'-phosphate binding.3. A protein that can perform pyridoxal phosphate binding, adenosylmethionine-8-amino-7-oxononanoate transaminase activity in cytoplasm to support biotin biosynthetic process.4. The protein requires pyridoxal 5'-phosphate to activate a critical catalytic site.5. The designed protein should interact with enzymes and substrates within the biotin biosynthesis, Cofactor biosynthesis pathway to enhance or regulate its activity.6. The protein must exhibit the following characteristics: It is the only aminotransferase known to utilize SAM as an amino donor.; Catalyzes the transfer of the alpha-amino group from S- adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). The designed protein sequence is ",
    "response#1": "MLLAFCSNDYLGLANHPEVIAALRAGAERWGVGGGASHLVVGHSGPHHELELALAEFTGRPRALLFSTGYMANLGAVTALVGKGDTVLEDRLNHASLLDAGLLSGARFSRYLHNDPASLAARLDKAEGNTLVVTDGVFSMDGNLADLPALAAVAQARGAWLMVDDAHGFGPLGASGGGIVEHFGLGQEQVPVLIGTLGKGFGTAGAFVAGSEELIETLIQYARPYIYTTSQPPAVACATLKSLELLRRESWRRQHLAALIARFRHGAEALGLTLMDSFTPIQPILVGGSRQAVALAGMLRARGIMVGAIRPPTVPANSARLRVTLSAAHSEAQVDRLLEALGCL",
    "response#2": "MLLAFCSNDYLGLANHPEVIAALRAGAERWGVGGGASHLVVGHSGPHHELELALAEFTGRPRALLFSTGYMANLGAVTALVGKGDTVLEDRLNHASLLDAGLLSGARFSRYLHNDPASLAARLDKAEGNTLVVTDGVFSMDGNLADLPALAAVAQARGAWLMVDDAHGFGPLGASGGGIVEHFGLGQEQVPVLIGTLGKGFGTAGAFVAGSEELIETLIQYARPYIYTTSQPPAVACATLKSLELLRRESWRRQHLAALIARFRHGAEALGLTLMDSFTPIQPILVGGSRQAVALAGMLRARGIMVGAIRPPTVPANSARLRVTLSAAHSEAQVDRLLEAFGAPPVSAPSTPELLAFCSNDYLGLANHPEVIAALRAGAERWGVGGGASHLVVGHSGPHHELELALAEFTGRPRALLFSTGYMANLGAVTALVGKGDTVLEDRLNHASLLDAGLLSGARFSRYLHNDPASLAARLDKAEGNTLVVTDGVFSMDGNLADLPALAAVAQARGAWLMVDDAHGFGPLGASGGGIVEHFGLGQEQVPVLIGTLGKGFGTAGAFVAGSEELIETLIQYARPYIYTTSQPPAVACATLKSLELLRRESWRRQHLAALIARFRHGAEALGLTLMDSFTPIQPILVGGSRQAVALAGMLRARGIMVGAIRPPTVPANSARLRVTLSAAHSEAQVDRLLEAFGAPPASAPSTPESPESSESSESSGASETSEASESSEASETSETSETSETSATSETSETSETSETSETSETSETS",
    "response#3": "MLLAFCSNDYLGLANHPEVIAALRAGAERWGVGGGASHLVVGHSGPHHELELALAEFTGRPRALLFSTGYMANLGAVTALVGKGDTVLEDRLNHASLLDAGLLSGARFSRYLHNDPASLAARLDKAEGNTLVVTDGVFSMDGNLADLPALAAVAQARGAWLMVDDAHGFGPLGASGGGIVEHFGLGQEQVPVLIGTLGKGFGTAGAFVAGSEELIETLIQYARPYIYTTSQPPAVACATLKSLELLRRESWRRQHLAALIARFRHGAEALGLTLMDSFTPIQPILVGGSRQAVALAGMLRARGIMVGAIRPPTVPANSARLRVTLSAAHSEAQVDRLLEALAAEA"
  },
  {
    "reference": "MDSTNVRSGMKSRKKKPKTTVIDDDDDCMTCSACQSKLVKISDITKVSLDYINTMRGNTLACAACGSSLKLLNDFAS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Plays a role in the maturation of immature virions to infectious particles; May also participate in viral transcription.2. The protein should be designed to localize specifically to host cell cytoplasm, virion component, host cell nucleus in order to perform its role in viral transcription more effectively. The designed protein sequence is ",
    "response#1": "LRCLHGTTMIQKEHMIEMDVASENGQRALKENPDRAKVTQNRWGRSVVQLANNDARCCVHDAGCATNQFSSKSCGVFFTEGAKAQQAFKQLEAFMKAMYPR",
    "response#2": "LRCLHGTTMIQKEHMIEMDVASENGQRALKENPDRAKVTQNRWGRSVVQLANNDARCCVHDAGCATNQFSSKSCGVFFTEGAKAQQAFKQLEAFMKAMYPR",
    "response#3": "LRCLHGTTMIQKEHMIEMDVASENGQRALKENPDRAKVTQNRWGRSVVQLANNDARCCVHDAGCATNQFSSKSCGVFFTEGAKAQQAFKQLEAFMKAMYPS"
  },
  {
    "reference": "MEVGELLSVLVDVQIFGTDYSTLELSSIVNDTRKVTENGSFIAIKGENFDGHDALDDVIKAGAKLLIVEKLPENWKELDAMFVKVTSTYRAQAVLASKFFNMPSESLNLVAVTGTNGKTTTSTMISDLLNALGHKTGLIGTIHYKVDETYYPAVNTTPNALELQRLYNEMLIAGCKDAIIEASSHALSLGRIWYSDIDCAIFTNLTREHLDFHKTMDNYAYAKSLLFAQLGQQFHNGKPRLAIINLDDPYHEVMSQATGANIVNYSLKNSNATVFADNITNYRGATSFQLYYKGQTYSVTLPMLGEYNVSNYLAAFLCMTVYYGYDVAAVLTATEQFHGVAGRMQSINEGQNFTAIVDFAHTPDALERVLSELSERKQGKLIVLTGHSGGNRDSGARPEIGDLLFKYADEIVFTADNPRYESIDKIVSELVQQHDEKTFITIAKREEAIAYAVETAQTDDIILFAGKGGEAYQVIEDQYVPYNEVEVVREAIKNSLSK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a high affinity for meso-2,6-diaminoheptanedioate.2. The protein contains novel Mur ligase central, Mur ligase C-terminal domains that confer a unique function or activity.3. The designed protein must possess magnesium ion binding, ATP binding to support cell cycle, peptidoglycan biosynthetic process, regulation of cell shape under certain conditions.4. A protein that can modulate the overall flux of metabolites through the peptidoglycan biosynthesis, Cell wall biogenesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "VTGTNGKTTVATLLYKLFTGLGYTCGLLSTVQNEIAGRVVAATHTTPDPIELNALLADMVAAGCTHAFMEVSSHAIHQQRVAGLVFAGGIFTNITHDHLDYHKTFDEYIRVKKSFFDNLPATAFALSNADDKRGTVMLQNTPARKAYYSLRTLAEVKGKILENELTGLVMVVDDIEVHFRLIGEFNAYNLLAVYGAANVLLHQSPEDVSKALVEFTGVPGRFETVDAGQNFTVIVDYSHTPDSLENALSTINEFAEKRIITVVGCGGDRERGKRPIMAKIAADRSDLAILTSDNPRTEDPGVILREMEAGVAGRTYKKIEDRREAIRFAVRSAREHDIILIAGKGHETYQDVAGVKHHFDDREELRKAFG",
    "response#2": "GRMEPVTVPNSDLVVIVDYSHKPEALKEALRAVRGLCDRRLWVVFGAGGDRDPSKRPLMGAIATQLADEIIVTSDNPRSEVPIEIANEIAEGVGSSGTPYRIVLDRGEAIDTAITEAQPGDLVLIAGKG",
    "response#3": "VTGTNGKTTVTYLIQAIQEAGGLNTGLIGTMGMHYKNRSVPVDNTTPEAHLIHEHLAMMKKEGARSVVMETSSNALYDGRVRGCDFNIGIFTNLTEDHLDLHPTMKDYMYAKSLLFSQLGNSYGNPRELKAAVLNADDEASDVYRHMTAAHVLTYGIENPADFQARDIRISPRGTAFTLIAQNQMYPVTMKLIGKFNVYNALAALAAVSGLVQDSREVGPGDAFIALPGFGTHGLRFARRALDAGAAAIVYETPAPEDVEVPADAIAVPALRTRLGAMADRFAGVPAERLAAALAAFAGAKGRMERIDCGQDFLAVVDYAHKPAAVAAVLSTLRAQVDGRIGVVVGCGGDRDASKRPIMGAEAARGADFVVVTDDNPRSEDPAAIRAAVVAGAREAETNAEIVEYASRREAIEAAVAWAGSGDAVVVVGKGHETGQIVGDQVLPFSDHAVLRDALEARA"
  },
  {
    "reference": "MSRYEKLFSSLAARKEGAFVPFIMLSDPNPDTALAIVRAAVAGGADALELGVPFSDPVADGPTIQASHIRALAGGATVDSAIEQIRRIRNEFPDLPIGMLIYGNVPFTRGLETFYSEFQEAGADSILLPDVPVREGAPFVAAAEKAGIDPIFIAPAQASEQTLQGVAQYSKGYIYAISRDGVTGTEKESETRGLDEVVNNVKRFGGAPILLGFGISTPQHVADAIAAGAAGAITGSALTKIVDAHLDEEGRPADGLAAAVTGFVSKMKAATKHAES",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-   glyceraldehyde 3-phosphate + H2O + L-tryptophan.2. The designed protein should interact with enzymes and substrates within the L- tryptophan from chorismate: step 5/5, L-tryptophan biosynthesis pathway to enhance or regulate its activity.3. The protein must exhibit the following characteristics: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.. The designed protein sequence is ",
    "response#1": "MTITTPARRHLAAAPDPDQAATLTAVRSRAFHLPTGTPYPAGTTPALVTPAAG",
    "response#2": "MPEDTSAPRPALVTGFEPFDGRPVNPSWEAVKRLDTRWLPGLRLRTLPVPFAKAAPVLAEAIRAHEPDLVLCILELGVPFSDPVADNRVQNVLIDDAAGFRDNDGTPIRSGPVADGPAAYFSTLPIKAMTDGLRAAGYDADVSYFAGTYVCNHLMYHVLDDAAAAGHPVRGGFIHVPYLADTPARPASPDQAAEGIRLALRAITDG",
    "response#3": "MDYILGIDPGSRITGYGVIRQVGRQLSYLGSGCIRTSSDSLPEKLRMIFAGLSEIITQYQPDEFAIERVFMAKNADSALKLGQARGAAIVAAVNRGLPVFEYAARQIKQTVVLELGVPFSDPVADAQVAIALLHTSAQMNLSDNDEHFEANAVLSDQ"
  },
  {
    "reference": "MSEEHIYPVTDNLAKNSLLTNEEYLTQYQASISDPSAFWGEKGKILDWIKPYTKVKNSSFDSGHVSIKWFEDGKLNVSANCIDRHLATKGDQVAILWEGDTADKDEKITYKQLHQRVCQFANVLKSQGVRKGDVVCLYMPMTPEAAVAMLACTRIGAVHSIVFGGFSPDAIAGRIVDSSAKIVITADEGRRGGRVVPLKANVDEALTKDGTDCVKSVIVFKNTGGEVNWVVGRDLDWESVCADESSECEPEAMNAEDPLFILYTSGSTGTPKGVLHTTGGYLVYAAMTFKYVFDYQEGDIYWCTADVGWITGHTYSVYGPLANGATSLIFEGVPNYPTPARMSEVVDKHKVSILYTAPTAIRALMAKGDQAIEGTHRSSLRILGSVGEPINPEAWEWYYNKIGDERCPIVDTWWQTETGGILISPLPGATDLKPGSATRPFFGVQPAIVDSEGVVLEGEAAGNLVMLDSWPGQMRTLYNNHDRFEQTYFSTFKGMYFTGDGARRDSDGYYWITGRVDDVLNVSGHRMGTAEIESALVSHPKIAEAAVVGVPHEIKGQGIYAYVTLNEGEYPSPELYAEVKQWVRKEIGAIATPDILHWAEGLPKTRSGKIMRRILRKIATGESDSLGDISTLADPSVVEQLIKENRETS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be able to modulate acetyl-CoA biosynthetic process from acetate in a way that leads to a desirable outcome.2. The protein should have a high affinity for CoA, Mg(2+). The designed protein sequence is ",
    "response#1": "LFGHLTPDELATLVAGSECLWFDDGETILDAGRDVVSLGVVIEGAVVAELAGADGPGEVVGQLGSGETFGEMGLMSGEPVPAGFRASGRARVLLVPIALFRSLIMSDPGALQHIARTILG",
    "response#2": "LFQNFSPWELLKLIEKSLVKTFEPGDCIIGFGEPGKFLGIVINGEAEVSSITETGEKKRLTILQKGDMFGEMSLLTGEPTCADVSAFNKCDVFLLPQETFSSSLIVNPAAMKIMAKTLSED",
    "response#3": "LFQNFSPWELLKLIEKSLVKTFEPGDCIIGFGEPGKFLGIVINGEAEVSSITETGEKKRLTILQKGDMFGEMSLLTGEPTCADVSAFNKCDVFLLPQETFSSSLIVNPAAMKIML"
  },
  {
    "reference": "MKSQKNIRHLSLEELTNYFEQIGEKKFRAKQVYEWLWLKQAHSFEAMTNLSKELRTTLQEHFTLPALKVETTQYSEDGTVKSRFKTVEGHMVEGVLIPADERQTACVSSQIGCSLSCKFCATGYMERKRNLDYDEIYDEVVLLNQQANKVYNKKLSNIVFMGMGEPLLNYKNVLKAIEKITSPEGLGMSPRRITVSTAGVAKMIKQLGDDKVRFNLALSLHAANDVKRNEIMPINESNNIKALIEALNYFYKQTGNEITFEYILFQNFNDSIKDADELIKIYRQVPADLVNIIEYNPIDAAKFLKPDEETVEKFMNHLSKARVNARLRRSRGKDIDAACGQLANKNTLELVEKMNNEQLTMNN",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs..2. A key design requirement for the protein is the optimization of the Proton acceptor, S-methylcysteine intermediate to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.3. A protein that localizes to cytoplasm and can perform rRNA binding, tRNA binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "EEGRGTLCLSSQVGCTLACTFCHTGTQKLVRNLTAGEIVGQVMMARDRLGDYPGLTATDGAAVPETGRLVSNIVMMGMGEPLYNFDNVKQALLIASDGEGIGLSRRRITLSTSGVVPMIPRTGEETGVMLAISLHAVRDDLRDELVPLNRKYPLAQLLEACRTYPGISNARRITFEYVMLKGVNDSLAEARDLVRLLKGMPAKINLIPFNPWPGTAYECSDWDQIERFADVVNRAGYASPIPRKKGCAD",
    "response#2": "EEGRGTLCLSSQVGCTLACTFCHTGTQKLVRNLTAGEIVGQVMMARDRLGDYPGLTATDGAAVPETGRLVSNIVMMGMGEPLYNFDNVKQALLIASDGEGIGLSRRRITLSTSGVVPMIPRTGEETGVMLAISLHAVRDDLRDELVPLNRKYPLAQLLEACRTYPGISNARRITFEYVMLKGVNDSLAEARDLVRLLKGMPAKINLIPFNPWPGTAYECSDWDQIERFADVVNRAGYASPIRHPFPGPGLGVRILAEEKSRGTLCVSSQVGCALNCSFCSTAKQGFNRNLTTAEIIGQVWVAVRELSTHEGAHDKHLTNVVMMGMGEPLLNFDNVVSAMNIMMDDFAYGLSKRRVTLSTSGVLPDLERLRQVSPVALAVSLHAPNDDLRNELVPINKKYPLKQLMALCKIYFKDEPRRKVTFEYVMLKGVNDQPEHADQLIKLLHDVPAKVNLIPFNPFPMTQYQRSSQQAIDAFRDKLIAHGINTITRKTRGDWE",
    "response#3": "SQVGCTLACTFCHTGTQKLVRNLTAGEIVGQVMMARDRLGDYPGLTATDGAAVPETGRLVSNIVMMGMGEPLYNFDNVKQALLIASDGEGIGLSRRRITLSTSGVVPMIPRTGEETGVMLAISLHAVRDDLRDELVPLNRKYPLAQLLEACRTYPGISNARRITFEYVMLKGVNDSLAEATPFCSMCGPHFCSMKITEDDLRDYVAQRGLDDDTTALLQSLAESV"
  },
  {
    "reference": "MLNGKTIILGVTGGIAVYKAVELLRLYVKAGADVHVVMTKNAQQFVTPLTFQTLSGNSVHTDLFNLAQEQEIGHISLADRADLFVVAPATANFIGKVAHGLADDLLSTSVMATKAPVLLVPAMNVNMFENPIYQRNQDQLRADGYRMMDPVVGALACGWEGKGKMPDPSDIFDETAQFLVPGDFCGTRILITAGPTRENIDPVRYISNYSSGKMGYAVARQARRRGAQVTLISGPVQLTPPEGVRLVKVSSADEMYRAVMQHASESDVIVKAAAVADFRAAHCAQHKMKKGQSDELTLTLTKNRDILAELGQIKDAQILVGFAAETEDLVANAALKLRNKNLDMIVANDVTDPGAGFDVDTNVVKFLYADGSIDSLSQRSKDAVADELLDRIVSLRKGD",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must possess metal ion binding, FMN binding to support pantothenate catabolic process under certain conditions.2. The presence of Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.3. The designed protein must have a Proton donor that is structurally stable and resistant to denaturation.4. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.. The designed protein sequence is ",
    "response#1": "KRITLALTGGIALYKICDVVRGLRRLGADVKVAMTEHAAQFVTPLTFEALSGHPVATTEWQPTPDGTMPHIDLTRGADLLLVAPATANILAKAAHGIADDLVSTLIAARRCPVVFVPAMNVNMWRNAPNRRNVELLREAGARFIGPVAGAQACGDEGEGRMTEPADILDRIAARLLDPKLEGVRVLVTAGPTRERIDAVRYITNPSSGKTGFSIAEEIARRGGEVTLVSGPVALPDPFGVQVVRVESALEMRDAVDAAYDACDVVVATAAVSDFRPAEPVDGKSKKDSAPLDIPLKRNPDILAGLGKRKGNRILIGFAAETDDVIDYATSKLFAKNLDLVVANDVSDPAAGFGTDANRVWLVTSEGVEDWPEMSKGAVAERLAERIAQHFQTTA",
    "response#2": "TAVVGVTGCIAAYKTCEIIRGLQKAGVRVKVVMTEHATKFVDPVTFRALTHEEVAIDLFDDPNDPIHHISLAEEADAFLVAPCTANVAAKMAHGIADDLLTTTALATTAPVLIAPAMNVHMYQHPATRANLGLLQSRGAIVIEAEDGYLACGEIGPGRLADPAAIVDATLALLARDDELAGTRIIITAGPTREALDPVRYLSNHSSGKMGYALAQAAVDAGATTTLVSGPVSLTPPEHVRCLRVESAGEMLEKCLELVPECDIFIACAAVADYRPAAIEQQKIKKGPEQVTLHLVRNSDIVAVVAAGDNSPFTVGFAAETNDVLNYARDKMQRKGLDMIVANDVSDPSIGFNSDHNEATVLFADGRETALPKADKLTLARQLVALIADRLETRA",
    "response#3": "KKILLIISGGIAAYKSLDLIRRIRERDASVQVILTNSAIQFITPLSVATLSGEPVLGDLFDLTRESEIGHIELSRSADIILVAPATANLIAKMAHGLADDLATTTLLATDTPVIVAPAMNVRMWEHPATQRNLSTLKSDGIELVGPDKGTMACNEYGMGRLDDPEIIFQEVAHIELAKNASILVVAPASANTIAKFANGIGDNILTDVYLANTAPVLLAPAMNTNMLEHPATKNNLNILFKRGVNFIEPISGNLACGTSGKGKMANPIKIVEKINDLAGESLLITAGPTQEPIDPVRFLSNPSSGKMGYALARAARRRGADVVLISGPVSVDAPIDVELIRVRTAQEMKEAVLSRFDSASIIIKAAAVSDYRPVNVSDHKIKKADETLALELSRTPDILRRLGEIKGDRLLVGFAAETEDLVKNAATKLKEKNLDLIVANNVQMSDSGFGVDTNRVTLIDKNGEEEELPLLSKREVAERLLDRIIELRGR"
  },
  {
    "reference": "MKIVFATHNTNKFKEVKALVPTTITLVSLTDIGCFDDIPETADTIEGNAIQKASYVTEKYNLPCFSDDTGLLVNALNGEPGIYAARYAGEQKSAEDNMAKLLHKLGDNPNREAHFKTVIALHINGEQHIFTGVVNGVITKGKQGTDGFGYDPIFTPEGYSQTFAQLPLNTKNKISHRAKATQQLISFLKQYNNG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be able to bind Mg(2+) ligand in a variety of conditions2. The protein requires Mg(2+) to activate a critical catalytic site.3. A protein with metal ion binding, ITP diphosphatase activity, XTP diphosphatase activity, nucleotide binding is needed to enhance nucleotide metabolic process, purine nucleoside triphosphate catabolic process in a specific context.4. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP The designed protein sequence is ",
    "response#1": "MRILISNDDGIFAPGLAALVKALK",
    "response#2": "MKIGILAGGTSAEREISLNSGK",
    "response#3": "MPGPKDPKRR"
  },
  {
    "reference": "MSKKLTVAVVGATGAVGREMLKTLHERDFPATEIRAFASARSAGTKVPYGDKELTVQELKEDVFEGIDLAIFSAGGSTSQKFAPHAAKAGCVVVDNSAAWRMDDRCPLVVPEVNAHALEGHNGIIANPNCSTIQMLVVLKPLHDAVKIKRVVVSTYQAVSGTGQKGIEELERQVRDLFNGRDPECNTYPYRIAFNALPHIDVFLDNDYTKEEMKMVHETVKIFEDPSVKVTATCVRVPVFYCHAESVNIETEKKLTAKDARVMLSQAPGVRVVDNPRELMYPMPSYCVGEDETYVGRIREDETIENGLNLWIVADNVRKGAALNAVQIGEELIKRDLLRVTDKNVFLK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should contain one or more Semialdehyde dehydrogenase NAD-binding domains that are essential for its function.2. A protein with NAD binding, NADP binding functions is required to enable diaminopimelate biosynthetic process, 'de novo' L-methionine biosynthetic process.3. The designed protein should have a high specificity for phosphate binding, with minimal off-target effects.4. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate. The designed protein sequence is ",
    "response#1": "LGGLFENNLVEWITSMTYQAASGAGAKNMEELVAQMAAIGNQAAGLLDDPSSAILDLDRKVTDTIRSKAFPTDNWGVPLAASLIPWIDRAMENGQTREEWKGFVETNKILGRSDNPIPIDGQCIRIGSMRCHSQAFTIKLNKKVPVGEIESMLAANNDWVKVVPNRKETSITDLTPAAVSGTLTVPVGRIRKMTIGDDYLTAFSVGDQLLLQGGAR",
    "response#2": "MNLAVVGATGAVGRIVLEQLALRKLPINRLKLLASQRSVGKEVRFADETLKVQLLEPDAFQGIDIVVASTPDEVSAEFASWATDAGAVVVDESGYWRMDPKVPLVIPEVNPEAIHDPPKGIIANPNCTTMAAMPVLKPLHDAARIKRVVVSTYQSVSGAGKDAMDELFRQTRAVFVADPIEKEKFTKQIAFNVIPHIDVFLDSGFTKEEWKMMVEVQKILDPDIQTTATCVRVPVFVGHSESVNIEFENAITAEKARSILREAPGVLVIDKREDEGYITPVECAGEDATYVSRIRKDPTVEHGLMLWVVSDNLRKGAALNAVQIAELVVK",
    "response#3": "LAPLHKKYNIKRVIVSTYQSITGTGVKAVQQLENEYAGVAGEMAYKYPIHRNAIPQCDSFEENGYTKEEMKLVRETKKILSDNTIAVTATAVRVPIVGGHSEAVNVEFSNDFDVDEVRNILHHTDGVVVQDNNDTYTYPMPMYAQGKDAVFVGRIRRDESQENTLNMWIVADNLREMPLLEGRMPLAPLHKKYNIKRVIVSTYQSITGTGVKAVQQLENEYAGVAGEMAYKYPIHRNAIPQCDSFEENGYTKEEMKLVRETKKILSDNTIAVTATAVRVPIVGGHSEAVNVEFSNDFDVDEVRNILHHTDGVVVQDNNDTYTYPMPMYAQGKDAVFVGRIRRDESQENTLNMWIVADNLRESTPGLRQYVGERLGGLFENNLVEWITSMTYQAASGAGAKNMEELVAQMAAIGNQAAGLLDDPSSAILDLDRKVTDTIRSKAFPTDNWGVPLAASLIPWIDRAMENGQTREEWKGFVETNKILGRSDNPIPIDGQCIRIGSMRCHSQAFTIKLNKKVPVGEIESMLAANNDWVKVVPNRKETSITDLTPAAVSGTLTVPVGRIRKMTIGDDYLTAFSVGDQLLRRLEE"
  },
  {
    "reference": "MDTRVDTDNAMPARFSAQIQNEDEVTSDQGNNGGPNGGGRLAPRPVFRPPVDPASRQAFGRPSGVQGSFVAERVRPQKYQDQSDFTPNDQLADPVLQEAFGRPFAGAESLQRHPIDAGALAAEKDGAGPDEPDDPWRDPAAAAALGTPALAAPAPHGALAGSGKLGVRDVLFGGKVSYLALGILVAIALVIGGIGGVIGRKTAEVVDAFTTSKVTLSTTGNAQEPAGRFTKVAAAVADSVVTIESVSDQEGMQGSGVIVDGRGYIVTNNHVISEAANNPSQFKTTVVFNDGKEVPANLVGRDPKTDLAVLKVDNVDNLTVARLGDSSKVRVGDEVLAVGAPLGLRSTVTQGIVSALHRPVPLSGEGSDTDTVIDAIQTDASINHGNSGGPLIDMDAQVIGINTAGKSLSDSASGLGFAIPVNEMKLVANSLIKDGKIVHPTLGISTRSVSNAIASGAQVANVKAGSPAQKGGILENDVIVKVGNRAVADSDEFVVAVRQLAIGQDAPIEVVREGRHVTLTVKPDPDST",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Essential protein that may act as a regulatory protease that is conditionally activated upon appropriate environmental triggers.2. The protein with the reaction Acts on substrates that are at least partially unfolded. The   cleavage site P1 residue is normally between a pair of hydrophobic   residues, such as Val-|-Val., the Charge relay system must be optimized to ensure efficient catalysis.3. The protein should have metalloendopeptidase activity and be active in plasma membrane, peptidoglycan-based cell wall.4. The target protein must exhibit helix-helix-strand-strand-turn-strand-helix-turn-strand-strand-strand-turn-strand-helix-strand as its primary conformation. The designed protein sequence is ",
    "response#1": "IVGGAPASIDSYPWLVVIEYVRLERTMLLCGGALISGKYVLTAGHCVKGAILDVGTPKTVRLGEYNTTNPGRDCVSVSAGGTDCTDPLVKIGIEKTIPHPDYQPYHFLRKHDIGLIRLQSIAPFTDFIRPICLPSTDYTVNPPSKFALTVAGWGRYLQFDNGTVRSSKIKLHVTLPFVQRDVCEANQKPLRNGQRITLWKGQMCAGGEAGKDSCKGDSGGPLMYEHSKKYEAVGIVSFGPEKCGQIDIPGVYTNVYEYLPWIQQHM",
    "response#2": "IVGGAPASIDSYPWLVVIEYVRLERTMLLCGGALISGKYVLTAGHCVKGAILDVGTPKTVRLGEYNTTNPGRDCVSVSAGGTDCTDPLVKIGIEKTIPHPDYQPYHFLRKHDIGLIRLQSIAPFTDFIRPICLPSTDYTVNPPSKFALTVAGWGRYLQFDNGTVRSSKIKLHVTLPFVQRDVCEANQKPLRNGQRITLWKGQMCAGGEAGKDSCKGDSGGPLMYEHSKKYEAVGIVSFGPEKCGQIDIPGVYTNVYEYLPWIQNTIR",
    "response#3": "IVGGAPASIDSYPWLVVIEYVRLERTMLLCGGALISGKYVLTAGHCVKGAILDVGTPKTVRLGEYNTTNPGRDCVSVSAGGTDCTDPLVKIGIEKTIPHPDYQPYHFLRKHDIGLIRLQSIAPFTDFIRPICLPSTDYTVNPPSKFALTVAGWGRYLQFDNGTVRSSKIKLHVTLPFVQRDVCEANQKPLRNGQRITLWKGQMCAGGEAGKDSCKGDSGGPLMYEHSKKYEAVGIVSFGPEKCGQIDIPGVYTNVYEYLPWIQNIVGGAPASIDSYPWLVVIEYVRLERTMLLCGGALISGKYVLTAGHCVKGAILDVGTPKTVRLGEYNTTNPGRDCVSVSAGGTDCTDPLVKIGIEKTIPHPDYQPYHFLRKHDIGLIRLQSIAPFTDFIRPICLPSTDYTVNPPSKFALTVAGWGRYLQFDNGTVRSSKIKLHVTLPFVQRDVCEANQKPLRNGQRITLWKGQMCAGGEAGKDSCKGDSGGPLMYEHSKKYEAVGIVSFGPEKCGQIDIPGVYTNVYEYLPWIQNTIK"
  },
  {
    "reference": "MELQPQFNEFLANIRPTDTQKEDWKSGARTLRERLKNFEPLKEIVVSTFLQGSIRRSTAIRPLGDKRPDVDIVVVTNLDHTRMSPTDAMDLFIPFLEKYYPGKWETQGRSFGITLSYVELDLVITAIPESGAEKSHLEQLYKSESVLTVNSLEEQTDWRLNKSWTPNTGWLSESNSAQVEDAPASEWKAHPLVLPDREKNEWGRTHPLAQIRWTAEKNRLCNGHYINLVRAVKWWRQQNSEDLPKYPKGYPLEHLIGNALDNGTTSMAQGLVQLMDTFLSRWAAIYNQKSKPWLSDHGVAEHDVMARLTAEDFCSFYEGIASAAEIARNALASEEPQESAQLWRQLFGSKFPLPGPQGGDRNGGFTTPSKPAEPQKTGRFA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: When the cdnD- cap2-cap3-cap4 operon is introduced in E.coli there is a more than 10(3) decrease in the efficiency of T2 plaque formation.2. A protein with ATP binding is needed to enhance defense response to virus in a specific context. The designed protein sequence is ",
    "response#1": "GLYVAAKFSDSTLDEIENLQRELKVPNPVPRHKIHSTICYSRVNVPYVVSTGSFEVANSGELQVWDTQDGRTLVLVLDSDYLKFRHNYARALGATHDFDDYSPHITLSYNVGPAQFSGIVQVPVILDREYKEPLKINWTEE",
    "response#2": "QFNKVTLDKISLFSKEILDLKINTVDEMKELASFVFVKAITEKAFLDTYTKLIGILKKNFKCAEEKNMGKESTCFFASLLKLMMKKIEENHTFNRDIYIDKENKTEIEIEEEIENAELARKLKRTQAIGAISFCVSLYKNSITGVANIKNTVNFLTKKPSSENIELLCEILRQGGPQLNINHSETVNQILHYIR",
    "response#3": "GLYVAAKFSDSTLDEIENLQRELKVPNPVPRHKIHSTICYSRVNVPYVVSTGSFEVANSGELQVWDTQDGRTLVLVLDSDYLKFRHNYARALGATHDFDDYSPHITLSYNVGPAQFSGIVQVPVILDREYKEPLKINWTEC"
  },
  {
    "reference": "MRFGGQRRCIESSDDNDSIDSMSIQQKNKEKEEWKWAQSLARRLEQVLNVHPEKYQNGCSSQRPSRQPSPVEQLAPIRSHSADFGRAAEMASGNSEEGKGMLLRNGDDRIRLPSPQRGTPDTSSTQGHPYTDQIVTINVSGMRFQTFESTLSRYPNSLLGDRNKRQHFFVSDTNEFFFDRHRTTSSSFTFEIRNYLFESILYIYQSGGRVKRPEIVPIDIFLKEMRFFQMGDDLLEEFWIAEGYEKPKEVMMPNNKTQRKIWELMEYPDSSLSARIIAFISIAVIALSIISFCWETVPSDIEEKPINNSATAELLDEMDEKHYSPFFWIELMCILWFTIELILRFISCPCKVTFATSVLNIIDFVAIAPFFVNFFFADTSKSNSSMSFAVLRVLRLVRVFRVFKLSRHSVGLQILGKTFRSSVQEFCLLIFFMAIALVLFASGMYFAEQGEPNSKFTSIPASFWFVLVTMTTVGYGDLVPLSPFGKVVGGMCAMIGVLTLALPVPIIVANFKHFYRQENRLASMKSKGDDADDDIA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Extracellular, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.2. The protein should exhibit Polar residues compositional bias.3. The protein should contain a Helical; Name=Segment S6, Helical; Name=Segment S5, Helical; Name=Segment S2, Helical; Name=Segment S1, Helical; Name=Segment S3 membrane-spanning region.4. For general function, the protein need meet that Has an important role in repolarization and in regulating the pattern of action potential firing; Mediates the voltage-dependent potassium ion permeability of excitable membranes; Isoform a expresses currents in a more depolarized voltage range than isoform d.5. The ideal protein would be one that plays a critical role in protein homooligomerization, potassium ion transmembrane transport, regulation of ion transmembrane transport through voltage-gated potassium channel activity, delayed rectifier potassium channel activity within voltage-gated potassium channel complex, membrane, plasma membrane. The designed protein sequence is ",
    "response#1": "VFYYVTGFFIAVSVMANVVETVPCGHRPGRAGTLPCGERYKIVFFCLDTACVMIFTAEYLLRLFAAPDRCKFVRSVMSIIDVVAIMPYYIGLGITDNDDVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLVFSLAMAIIIFATVMFYAEKNVNGTNFTSIPAAFWYTIVTMTTLGYGDMVPETIAGKIVGGVCSLSGVLVIALPVPVIVSNFSRIYHQS",
    "response#2": "IWDWVILCLTFYTAIMVPYNVAFKNKTSEDVSLLVVDSIVDVIFFIDIVLNFHTTFVGPGGEVVSDPKVIRMNYLKSWFIIDLLSCLPYDVFNAFDRDEDGIGSLFSALKVVRLLRLGRVVRKLDRYLEYGAAMLILLLCFYMLVAHWLACIWYSIGRSDADNGIQYSWLWKLANVTQSPYSYIWSNDTGPELVNGPSRKSMYVTALYFTMTCMTSVGFGNVAAETDNEKVFTICMMIIAALLYATIFGHVTTIIQQMTSAVTARNQYLNAKMSFLAFQSLGVVFGDLGTSPLYVFYNIFPHGIEDTEQVIGALSLIIYSLTLIPLVKYVFIVLRANDNGQGGTFALYSLLCRHAKINIIPNQHRTDQDLTTYSRRTYEEKSLAAKIQRWLEGHQFRKNLILILVLFGTCMAVGDGILTPAISVLSATGGIQVEEGRMRNDVVVIISVLILIGLFSMQHYGTDKVSWLFAPIVFVWFILIGILGAVNICKYDHSVLKAFNPVYVYRYFKRGKTSWTSLGGIMLSITGTEALFADLSYFPVQAIQIAFTVVVFPCLLLQYTGQAAFIAANTNQVSHAFYISLPAPILWPAFAVATAAAIVASQATISATYSIIKQALALGCFPRVKIIHTSKKYLGQIYSPDINWILMVFCIAVTAGFKNQSQIANAYGTAVIMVMLVTTFLMIPIMLLVWRSHWTLVVAFTVLSLLVEIPYFSAVVRKIDQGGWVPLVFAAGFMIIMYVWHYGTLKRYEFEMHSKVSMAW",
    "response#3": "RVVAFASLFFILVSITTFCLETHEAFNIDRNVTEIHRVGNITSVRFRREVETEPILTYIEGVCVMWFTLEFLVRIVCCPDTLDFVKNLLNIIDFVAILPFYLEVGLSGLSSKAARDVLGFLRVVRFVRILRIFKLTRHFVGLRVLGHTLRASTNEFLLLIIFLALGVLIFATMIYYAERIGARPSDPRGNDHTDFKNIPIGFWWAVVTMTTLGYGDMYPKTWSGMLVGALCALAGVLTIAMPVPVIVNNFGMYYLAFAGSTPGCLRALSMRFRTTHSPPGDTLVHRGDILTGLYFIARGSVEILNDDNTVMGILGKDDIFGENPLLYDEVGKSSCNVRALTYCDLHKILRDDLLDVLDMYPEFAETIEKEDKKSPPGDTLVHRGDILTGLYFIARGSVEILNDDNTVMGILGKDDIFGENPLLYDEVGKSSCNVRALTYCDLHKILRDDLLDVLNREDALVQEQWDNDFPIVYCNESFCKISGYNRAEVMQKSCRYVCGFMYGELTDKETVGRLEYTLENQQQDQFEILLYKKNNLQCGCALSQFGKAQTQETPLWLLLQVAPIRNERDLVVLFLLTFRDGNEISRPCTLDLLPGIGLVYTELASGVPHIFSHFITNLPAIHSTLVFVCVKYLPVYTVPPDERFLVKRIGPKNFHMFRCVARYGYKDIHKKDDDFEKMLFDSLILFVRLESMMEEYSDSDEYSTLMMSLPNNPGISNGGVTTTGTNNVMEVMSCTSTHDSIVPVNSRSDDTGSSQVMPASGQMAFQSVGDEIAFLNACRDAGVVHILGNTVIRARRDSGFVKKIVINYMYAFLR"
  },
  {
    "reference": "MATPKNLPSVFNATAQDIEMLLSAQAHIGSKNLQVHMEPYLWKTRPDGVNIINIGKTWEKIVLAARIIAAIDNPADIAVISARPYGQRAVLKFAAHTGASAIAGRFTPGNFTNYITRSFKEPRLIIVTDPRTDAQAIKEASYVNIPVIALCDTDSPTEYVDVAIPTNNKGRHAIGLIWWMLAREVLRLRGTLASRETEWDVMTDLYFYRDPEAEENKDSAGQDEAKVPGADEVGPGAVESGFNNEWEVGNAGSSAFAAASGTAGAQAASTWDAEGADWAAASAPATRAGLLTAPPALQLPPTPSVSILGKDTIIVDYGLWPNYATHDLLVNIPSSTYVLICDSNLAKLPYVPAFVESFNAERSRLGKNPEDVRLLVLDNIPPGETSKSRNVKAQIEDWLLSQGCTRDTVLLALGGGVMGDLLGYVAATYMRGIKFCQIPTSLLSMVDSSIGGKTAIDTPLGKNLIGAFWQPERIYIDLAFLETLDKRQVCNGMAEVVKTAAIWDEREFERLEEGADAIMSALERPLGKGRFAGIEDVFKRIVLGSARVKAEVVTADEREGGLRNLLNFGHSIGHAYEAILTPEILHGECVAVGMVKEAELARYLGILEPAAVARLTKAIKSYGLPTSLDDKLITSKTRKVCEADELLRRMAVDKKNAGSKKKIALLASIGKCYEPETGPGATKCDDKDIRIVLCPSVLVRPGVSKVLNVVCKPPGSKSISNRILLMAALGKGSCRITNLLASDDTQYMLSAIASLQGATYGWEDEGRV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should contain one or more 40S ribosomal protein SA C-terminal, Shikimate dehydrogenase substrate binding N-terminal, Enolpyruvate transferase domains that are essential for its function.2. For general function, the protein need meet that The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis.3. The Schiff-base intermediate with substrate; for 3-dehydroquinate dehydratase activity, Proton acceptor; for 3-dehydroquinate dehydratase activity, For EPSP synthase activity active site of the designed protein must be optimized for substrate binding and specificity.4. The protein design should consider the endogenous proteins and enzymes present in the Metabolic intermediate biosynthesis, chorismate biosynthesis pathway to avoid interference with native metabolic processes.5. The designed protein should have a binding site that is specific to the target ATP, Zn(2+) ligand.6. A protein that contributes to chorismate biosynthetic process, translation, aromatic amino acid family biosynthetic process by functioning in cytosolic small ribosomal subunit with shikimate 3-dehydrogenase (NADP+) activity, ATP binding, 3-phosphoshikimate 1-carboxyvinyltransferase activity, 3-dehydroquinate dehydratase activity, metal ion binding.7. The presence of Zn(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "IKPGEGSKSRKTKAQVEDWLLQQGCTRDTVILALGGGVIGDMVGFVAATYMRGVRFVQIPTSLLAMVDSSIGGKTAIDTPLGKNLIGSFWQPERVYIDLQFLETLDKRQVDLNAVAASPEFKGGMAEVVKTAAIWDEQEFAFLEDNAATILDSFRAPHNPQDPGARLLPVRATLKRIVLGPARVKAEVVSKDEKEGGLRNLLNFGHSIGHAIEAILTPQVLHGECVAIGMVKEAELARFLGVLKPGAVARLTKCIASFDLPTSLQDKRVVKLTAGKQCPVDTLLDKMAVDKKHGFDGTEPVTVVGMAEVVKTAAIWNEEEFTRLENFSKKFLTVVTAKNPDLLSIKDELVKTVLESVRVKAEVVSSDEKESSLRNLLNFGHTIGHAIEAIVTPEALHGECVSIGMIKEAELARYWGVLPPVAVARLTKCLVAYGLPVTIDDKEFLQRVGPKRHNIEIDLLLKKMSIDKKIVDDKKRDLDKGIAEIIKAGMCSRADLFAELESTLSSKGVEGLIQDTERLRDMIVAGIDYKRSVVEEDERDTGIRNELNWGHTVGHAIEGMGVTGLHHGECVSIGMVYEAMALRARGQLSNIAVQRLEKVLKCCDLPTFLPPGAAANQQELMRRMKRDKKLAALDKK",
    "response#2": "GMAEVIKTACIWNADEFIRLEVNADPFLEIVNNCQSITHKNKITGNIEKICDCKIEDILDHVYRLVLESIKVKAEVVSSDERESSLRNLLNFGHTVGHAYEAILTPQALHGECVSIGIIKEAELSRYLGILSTTQVSRLYKILVAYGLPVSPNEQWFKELTLQKKTPLNVLLDKMSIDKISSYL",
    "response#3": "YTIPPGETSKGRETKAEIEDWMLSHQCTRDTVIIALGGGVIGDMIGYVAATFMRGVRFVQVPTTLLSMVDSSIGGKTAIDTPMGKNLIGSFWQPRRIYIDLAFLESLPVREFYTIPPGETSKGRETKAEIEDWMLSHQCTRDTVIIALGGGVIGDMIGYVAATFMRGVRFVQVPTTLLSMVDSSIGGKTAIDTPMGKNLIGSFWQPRRIYIDLAFLESLPVREFPVELFGHPKEELRVYLGNAGTAARFLTGMAEVVKTAAIWDEQEFAFLEDNAATILDSFRAPHNPQDPGARLLPVRATLKRIVLGPARVKAEVVSKDEKEGGLRNLLNFGHSIGHAIEAILTPQVLHGECVAIGMVKEAELARFLGVLKPGAVARLTKCIASFDLPTSLQDKRVVKLTAGKQCPVDTLLDKMAVDKKGMAEVVKTAAIWDPVDFAKLESSAPAIRSAVLGPFAKNAPIDQGRTLQTRTESQSLLLDVIRGSVGVKAHIVTIDEKETGLRNLVNFGHTIGHAIEAVLTPEMLHGECISIGMILEAEVARYMHGLSQVAIGRLTRCLKEYDLPVALSDSRVTRLPKSLDVTIDRLLDIMKVDKKMR"
  },
  {
    "reference": "MKLFGLIGEKLGHSLSPEIHNKVFKDNNIDGLYNLFSVKKDFENNIVESLKCLGVRGANVTIPYKEKVMNQLDIISHEAKAIGAVNTILIKDGKSYGYNTDYYGFGKMLERAKVDIEGNSFFVLGAGGAARSILKYLEDSKAKKIVLVSRDKEKVFKKFKDFNINFMSYGELEEIHEEFALINTTPCGMYPNTNSVAVSEKVIKKFKVALDIVYNPLETKFLKMAKDNGLKTVDGLFMLVGQGVKAEEIWNGIKVDKSTEENIYEELKCRF",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The Proton acceptor of the protein must be engineered to enable effective catalysis of NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH.2. The protein design should consider the endogenous proteins and enzymes present in the Metabolic intermediate biosynthesis, chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7 pathway to avoid interference with native metabolic processes.3. The protein must exhibit the following characteristics: Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids; Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA)..4. The designed protein should have a high specificity for shikimate, NADP(+) binding, with minimal off-target effects.5. The designed protein should be optimized for NADP binding, shikimate 3-dehydrogenase (NADP+) activity activity in order to improve aromatic amino acid family biosynthetic process, shikimate metabolic process. The designed protein sequence is ",
    "response#1": "MIGVFGNPVAHSKSPRIHQLFARQTGIEHRYGKILVPISKFQEALDTFLKQGGIGVNITVPFKEQAFIRANELTERARLSGAVNTLKREGNEIIGYNTDIAGFTAPLEEEIGKFNNKNILMIGSGGSSRSISFELAKENEITIINRNIKNAKELSFEISKNLATKEYGEKYLKYGDLSTDISKFDIIINTTPAGMAGRIDSLPEEIFAPGTLVADIIYNPLETKWLKAAEDKGCKAINGTDMLVYQGVAQLEWWLSRPIPEFPCVAAMKQAVNQLK",
    "response#2": "METANILLIGSGGAAQSIIPVLLDIKKCCVNIINRTFFNAQRLVQYYHSIGRQNISCIDLNNLLCEKNACKKYDLIINASSSSSLFTIKGQKVIILGAGGVARAVAFLMASEGADIIYILNRTVERAREVADEVAAKVPGSRFEVLALDKYDEIPRDRYLCIQLANEKFVYSNGTDMLVYQGVAQLEWWLSRPIPEFPCVAAMKQAL",
    "response#3": "MAGLIGRAVDYSYSPLIHNTACRLLGLSYHYTVFNIADPSMIPDALRGAKALGIAGFNVTIPYKKSVVPFLDALSAEAASIQAVNTLALEDGRMVGYNTDGQGLLRALETANILLIGSGGAAQSIIPVLLDIKKCCVNIINRTFFNAQRLVQYYHSIGRQNISCIDLNNLLCEKNACKKYDLIINASSSAGXSGXLPXLPXAFLSPXCLVXDIVYNPLETRLXREAAXRGCRTVNGLDMLVYQGAAALEIWLQQPVSATVMSEALRSALRS"
  },
  {
    "reference": "MVTTLPQAFYSNFLGNAPSWYKKAICLFLLINPVIFLISPYIAGWVLILEFIFTLAMALKCYPLQPGGLLAIEAVLIGMVSPHTVYEEVSGNLEVILLLVFMVAGIYFMKELLLHLFTKILLSIRSKAILSLLFSLVAAVLSAFLDALTVTAVIISVAVGFYSVYHKVASGKHFHHDHDHGDDESVHHDHRADLEQFRSFLRSLLMHAAVGTALGGVCTQVGEPQNLLIAQRLGWDFIEFFVRMAPISIPVLIAGLITCVILEKTRWFGYGDDIPASVRKILEDFNEAEKKKMTLQHESKLITQAIVALILVVALALHLAEVGLIGLTVIILATAFCGVIEEHQIGKAFEEALPFTSLLVVFFAVVGVIHEQHLFTGIINYVLGLEKSVQPGMFFIANGVLSMISDNVFVATVYIDEVRTAFNDGLIDRAHYEALAVAINTGTNLPSVATPNGQAAFLFLLTSAIAPLIRLSYGKMVWMALPYTLVMGGLGYVMIVITI",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The protein have enhanced 3 H(+)(out) + 2 Na(+)(in) = 3 H(+)(in) + 2 Na(+)(out). The designed protein sequence is ",
    "response#1": "ETPFAP",
    "response#2": "MTERR",
    "response#3": "EXPXR"
  },
  {
    "reference": "MKHMRRLFSLLSLVLLLWGSAVAQDYTVGQLLRDLDAKRVREVSFDGNGNATVTLVGDRREVTVVPPTADFLTRIRESGAEVNVRSARSPFAWVFQLLPIVLLGLFLFVMWRSMRGAASGGASNTFGRSRAHVFAEGSVKVTFTDVAGCDEAKADLQEVVEFLKSPERFHQLGARIPHGILLVGPPGSGKTLLAKAVAGEARVPYFSISGSDFVEMFVGVGAARVRDLFEQAKKAAPCIVFIDEIDAVGRKRGMNFNGGNDEREQTLNQLLVEMDGFESKHDIIILAATNRPDVLDAALLRPGRFDRQVVVDAPDVRGREYILKIHARKKPLDPSVDLGVVAQRTPGMVGADLENLLNEAALLAARDGRTRITMTDLDDAADRVLMGPARKSRLVPEHDRKVTAYHEVGHALVAQLLPHADRVHKLTVVPRGRAAGYMLPLPTDRIHYTREVLEDTIAVALAGQAAEEVTFGEITTGAQSDFQKATNIARKMVTEWGMSQRLGKVANLSENDTFLGGFTERAPYSQLTAQAIDDEVKRIIDEQYARVKALLIEHLAKIHTAVDALLVRETLSGQEFSTLLAGGTLDALPAPKSRPTSSA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The designed protein should contain one or more AAA+ ATPase domains that are essential for its function.3. The protein should be able to bind Zn(2+) ligand in a variety of conditions4. The designed protein must have a active site that is structurally stable and resistant to denaturation.5. The presence of Zn(2+) is crucial for the protein to function optimally under specific conditions.6. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins7. A protein that can perform metalloendopeptidase activity in plasma membrane to support proteolysis, protein catabolic process.8. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "MVMSEEEKLKTAYHESGHAIVGRSLEEHDPVYKVTIIPRGRALGVTMFLPEEDKYSYSHQSILDRICGLYGGRIAEELIYGEKGVTTGASNDIERATELAHNMVTKWGLSEKLGPIKYGDETDEPFLGRSAGASKQGISDDTAHEIDNEVKTILSNCYQHAKDILSENIEALHAMSKALMDYETLDVDQIDDATLAEVQF",
    "response#2": "MVMSEEEKLKTAYHESGHAIVGRSLEEHDPVYKVTIIPRGRALGVTMFLPEEDKYSYSHQSILDRICGLYGGRIAEELIYGEKGVTTGASNDIERATELAHNMVTKWGLSEKLGPIKYGDETDEPFLGRSAGASKQGISDDTAHEIDNEVKTILSNCYQHAKDILSENIEALHAMSKALMDYETLDVDQIDDMEIVRL",
    "response#3": "MVMSEEEKLKTAYHESGHAIVGRSLEEHDPVYKVTIIPRGRALGVTMFLPEEDKYSYSHQSILDRICGLYGGRIAEELIYGEKGVTTGASNDIERATELAHNMVTKWGLSEKLGPIKYGDETDEPFLGRSAGASKQGISDDTAHEIDNEVKTILSNCYQHAKDILSENIEALHAMSKALMDYETLDVDQIDDARCRLNELIPRGVLLVGPPGTGKTLLAKAIAGEAGVPFFSMSGSEFVEMFVGVGASRVRDLFRKAKESAPCIVFIDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNSGVIIIAATNRPDVLDSALMRPGRFDRQITVDLPGVEESVGIVTARASGMGLENSVIVIAATNRPDVLDPALIDHKTGKPLDYTIDNLVAALGRYDPPKPDGTTL"
  },
  {
    "reference": "MGYRIAVMGATGAVGRETIKTLAERGFPASEVIALASARSVGQEISYGEKTILKVRNLDTFDFTGTDLVFGAAGASVSAIHAPRAAAAGAIVIDKTSQFRMEPDVPLIVPEVNPHHLSQVRRRGIIAVPNCSTIQMVLALKPLHQIARIKRVVVSTYQAVSGAGKEAMDELFNQTRGVYVNDPPTVEQFTKPIAFNCIPHIDSFMADGQTKEEWKMAVETRKILDPDIKVIATCVRVPVFIGHAEAINVEFETAITEKQAWDAWRGQEGLTVLDHREDGGYATQTETAGEDPVFVSRLRRDPTVEHGLVFWCVADNLRKGAALNAVQIAELLVKEGMLGKKKAA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be optimized for protein dimerization activity.2. The designed protein should be able to compete with endogenous proteins or inhibitors for phosphate binding.3. The designed protein must have a Acyl-thioester intermediate active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "LGGLFAEDLVEWVSPMTYQAASGSGAKHMRELITQMGEINEAVANQLDNPASAILEIDKLVAEKMRSDEFSTDQFGVPLAGSLIPYIDTQLPSGQSREEWKAGAETNKILGTQDQAIPIDGLCVRVGAMRCHSQALTIKLKKDLPVEEIEAKLAAANDWVKVIPNDREASINELSPTVATGTLSIPVGRIRKLEMGPEYISAFTVGDQLLK",
    "response#2": "LGGLFAEDLVEWVSPMTYQAASGSGAKHMRELITQMGEINEAVANQLDNPASAILEIDKLVAEKMRSDEFSTDQFGVPLAGSLIPYIDTQLPSGQSREEWKAGAETNKILGTQDQAIPIDGLCVRVGAMRCHSQALTIKLKKDLPVEEIEAKLAAANDWVKVIPNDREASINELSPTVATGTLSIPVGRIRKLEMGPEYISAFTVGDQLLY",
    "response#3": "LGGLFAEDLVEWVSPMTYQAASGSGAKHMRELITQMGEINEAVANQLDNPASAILEIDKLVAEKMRSDEFSTDQFGVPLAGSLIPYIDTQLPSGQSREEWKAGAETNKILGTQDQAIPIDGLCVRVGAMRCHSQALTIKLKKDLPVEEIEAKLAAANDWVKVIPNDREASINELSPTVATGTLSIPVGRIRKLEMGPEYISAFTVGDQLLP"
  },
  {
    "reference": "MERVHLTAWLALFLIVVANATPTPARTPTGCPADCTCSLSQHTHKPLYHLKCNSTRGLRLTEKTFQSTVPVHSIDLSHLNLTRLSHLLDKLPELTSADLSHNQLKDLGHLGKGLKRLNLKHNQLTSDKLRKLPQHLQVLNLQHNNITHLPLELTHMHQLHQLELSHNAINCSCQTLEVRNWLVERIVYMEHPVVCSYPLEFRGRSWLQLKQDEICKKEKYQWFDTEENELMMGDQPAAVSAEREDEEELGKDFLPIVGNPAATAKKVRSPQIPLPSDQVEGSGDLSETNMELKLPEETVAEPEAAESQLVDAAASPSVLEEHIVKDEDEDDEGSGSGGGLLIIPDPSKVKITSEDDIDSDGKPEESDVRPLENPENSENPDTVFSNKIGIYEGDQEEKKPVEEDNIVPVVMTNLDTGLESDVVTDGPLDSSKESEDILTAKIGKPKDDSSAIYYLLAVIGLIVVGLVLFVAIKRCKYDSNAAARDAEAQRQTELLDMDKKQLGKPLHKNGHGNGQEHSPLIGEKTKLDEAQIVKKPYENGEAKDGAGQQPLLNGNGSANGGTKEAPETGEPAAHEYYPITPRYPTPQSPRASKYAQQQQLAEQNNNEPDGAYLPSSPKSGRYSPVYSPETGRVKIKLTETPKPKTPMLVTRSKSNAGDIITTPVRPIEPTHQVINGH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Extracellular topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.2. For general function, the protein need meet that Plays a role in negative regulation of the JAK/STAT pathway by binding to the receptor dome and promoting its internalization for subsequent lysosomal degradation, thereby reducing JAK/STAT signaling.3. The designed protein should have at least Helical transmembrane domains to ensure proper localization.4. Design the protein with a signal peptide incorporated.5. The designed protein must have at least one LRRCT domain.6. The protein should be optimized for stability and solubility when expressed in plasma membrane, membrane where it will be involved in negative regulation of stem cell proliferation, synaptic target recognition, trachea development, positive regulation of endocytosis, negative regulation of receptor signaling pathway via JAK-STAT. The designed protein sequence is ",
    "response#1": "LMNPFSVNFENVNATNAIMTWKVHSIRNNFTYLCQIELHGEGKMMQYNVSIKVNGEYFLSELEPATEYMARVRCADASHFC",
    "response#2": "LSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHGEVALYKNLLHSKESSCSDSTNEYMDMKPGVSYVVPTKADKRRSARIGSYIERDVTPAIMEDDELALNGIHGPLQESYVLSKLKREEVEDGLYLIRWSSFDFHRLILTVARKDQAQGLRYKQFRIEEQGRTFQLEGWDQTFSSVRELTTALQGCTLRSSSGSGPGSSLESFTISKCCLV",
    "response#3": "LIIKASLGQGTFTKIFCGVRKELGDYGEMHQIDVVVKILDKAHRNYSESFFEAASMMSQLSHKHLLLNYGVCVCGDQNMMVQEYGKFGSLDTYLKKKKSCVNITWKLEVAKQLAWAMHYLEDKNLIHGNVCAKNVLLIREEDRKSGSVPFVKLSDPGISLTVLPREVLVERIPWVPPECIETPQKASLATDKWSFGTTLWEICSGGDKPLSTLDCSKKFLFYEC"
  },
  {
    "reference": "MLPAYLPNPAAALFGGGTPIDGGRRWSDGRRLLGDGKTWRGLVLGILSGVLLGLIQVSVQDACVFVWLPRHTVLSVLLLAVGALAGDMVKSFVKRRIGKERGAAWPLADQYDLVAGSLLLLLIGDYGFAAVNLTIPVIFWILVLTPLLHRAVNLIGYAIGVKDVPW",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design should consider the endogenous proteins and enzymes present in the Membrane lipid metabolism, glycerophospholipid metabolism pathway to avoid interference with native metabolic processes.2. We are looking for a protein with broad substrate specificity for 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + CTP +   H(+) = CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + diphosphate.3. For general function, the protein need meet that This reaction is the third ether-bond- formation step in the biosynthesis of archaeal membrane lipids.; Catalyzes the formation of CDP-2,3-bis-(O-geranylgeranyl)-sn- glycerol (CDP-archaeol) from 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1- phosphate (DGGGP) and CTP4. The ideal protein would be one that plays a critical role in glycerophospholipid biosynthetic process through CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase activity within plasma membrane.5. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.6. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "MTASPTSEASSSASTASPAAASPTSTSPAGASASASPASASASASPASAPSAAGASAGPGSASASAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGRGAGAGAGAGAGAGAGAGAGAGAGRGAGRGAGAGAGAGAGAGAGAGVGRGSGVGAGGGAGAGAGAGAGAGAGAGAGAGAGAGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGVGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS",
    "response#2": "MADLGAGDVNLEVVRDGASGAGGQQNIFVETLGCVWLSTKDRSSEDNCSEPPIEFNFKPTEPVIVKEKRCRKIVEGPPADDAITEETFMKIHELKMFLCDRVIEANYNCYIFGLPKRVGKELLLKSVMSHFYGKVNSVEDILEKMNLVEKRSAEEIEATLSPQKKMKTESVDEEVFPLLYLIKEPDIFDSDCEGFLKIIYDHYENYPPPIVVIMHDISKLLPIGVETKFQLSEEYQKIHKGKMFLIATSDTLQEEYLEIFDNATEYVSLPPPDGPTLLEIIRKSLTLKKGHYLPDDLFLGLMKKVGYDSHKILQLIKMIPTYPQLDSIIDANGLPEPHVTLQKLFDQCQQTKSQESKKKVKSKDKKKSPPLKKGKSASKKQKGKDKNKKSDTSDTKHESSDKKDEEPTSESEPEPEKEPELDSESDSDADSGSDSEEDESDNVEGSDSDSGSESDSGSDSDDDDDDD",
    "response#3": "MVRSIASLARSRGWPLWRPPQHYRGDFATSLGTLASGTAYKHHRQQQQQQEQQQEQQQERQQQSDQQHSSEPQRPRGHPQDHNESTTTNDNEESQKKQQSNKTLPAKERDFFSVQTFLDQLFSSKPQPDQNAPDLSARTYSLPDSQSSSQAPTAPPSRSGNPTFTLPNLPHLQTLAQKYGLTPKQLQNVWKIYPSRITPTIPPKLYQLAGLPRKIQIYLTQNPRVSGLSPLVTGSIALLYFLFIVAPERRQYQERWATERRERLMNATESSAPLPPQRSRSQVATEILQAGKFDPYAFHIIHTENDEQRKHRLRQNREEERKAWREEKERELELKRERERKEAEERKKQRNKEKEFQQALEAAKSQEASSSSSSATTPSA"
  },
  {
    "reference": "MRHYYTADAIRAAEAPLLASLPDGVLMRRAAYGLATVIAGELRTRFGAVAGRQVCAVVGSGDNGGDALWSATFLRRRGVTSTAVLLSPDHTHAKALAAFRQAGGRLVDGIPATTDLVIDGVVGISGRGPLRPEAATVFASSAAPVVAVDIPSGIDVHTGVADGPHVRAALTVTFGGLKPVHALGDCGRVELVDIGLELPPSAVRGLEAADVAARWPLPGPHDDKYSQGVTGVLAGSATYPGAAILCTGAAVAATSGMVRYAGSAAADVVTRWPEVVVAPRPDAAGRVQAWSVGPGLGTDESAAATLRFALAADLPVLVDADALTLLAAQPELVQTRTAPTVLTPHAGEYQRLAGHPVGPDRVGAAQELAARLGATVLLKGNVTVITDGDTTYLNPAGESWAATAGSGDVLSGVVGALLASGLPPIEAAAMAAFVHARAAGLAAADPGPRPVPISAEGILAHLRSSVAKL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.2. The designed protein should be optimized for metal ion binding activity in order to improve phosphorylation. The designed protein sequence is ",
    "response#1": "LVAGGEVMTGAARLAARAAARLGAGLVTVAAPEAAFPIYAAALTGVIVHPIADIEGFQGFLADKRRNAALIGPGAGVGDETRARTLAILGAGKRAVLDADALTSFAEDPGTLFSAIRSPCVMTPHAGEFSRLFDAAGDKPERARRAARQSGAVVLLKGADTVIAAPDGRVAVNANAPPQLATAGSGDVLAGIVLGLLAQDMDPFAAAAAAAWIHGAAASRL",
    "response#2": "PGDHKGSRGKVAVVAGSAGMAGAATLASRAALRGGAGMVMLGAPAGLMDALTARHTEVMLRGLPETAEGSLSLAAESDIDALLSWADVLAIGPGLTRQQETSALVRRVVSNSERPVVIDADGVNAFAGQPDGLAGTAPEVIMTPHVFELSRLTGMAADDIEADRVAAARQAAGSLQVTLVLKGAASLVASPEGQVSVNPTGNPGMATAGSGDVLTGLIAALLGQGLGAWDAARLGVYLHGLAGDLGAETMGPHSLVAGDLIDYLG",
    "response#3": "IGAGLVTLAVPGAALQENAARLDAVMLTRMNDVRDLAEILEDDRLNALCLGPGLGHGGGTRALVHAALEVERPVVLDADAMTSFEEDPEELFDALHLGVVLTPHMGEFARLFPDLAAPLKESPEAGPAYSRLDAARLAAERAGCTILLKGPDTIIANAESAALHGAFYDRAAPWLATAGAGDTLAGFITGLLARDDDPFFAATAATWLHADCARDFGPGLIAEDLPERR"
  },
  {
    "reference": "MPEVERKPKITASRKLLLKSLMLAKAKECWEQEHEEREAEKVRYLAERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVMDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.2. The protein should be optimized for stability and solubility when expressed in troponin complex where it will be involved in muscle contraction, transition between fast and slow fiber. The designed protein sequence is ",
    "response#1": "GEFKAAFDIFVLGAEDGCISTKELGKVMRMLGQNPTPEELQEMIDEVDEDGSGTVDFDEFLVMG",
    "response#2": "EEEELSDLFRMFDKNADGYIDLDELKIMLQATGETITEDDIEELMKDGDKNNDGRIDYDEFLEFMK",
    "response#3": "MPTIIPTPEELQEMIDEVDEDGSGTVDFDEFLVMMVRCMKL"
  },
  {
    "reference": "LTCVTSKSIFGITTENCPDGQNLCFKKWYYLNHRYSDITWGCAATCPKPTNVRETIHCCETDKCNE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: The toxin reversibly binds to ADRA2B, and its mode of inhibition is non-competitive.2. The designed protein localizes to the extracellular region. The designed protein sequence is ",
    "response#1": "RMDKAGQYTDKGLRKCCEDGMRDIPMPYSCQRRARLITQGESCLKAFMDCCNYITKLREQHRRDHVLGLF",
    "response#2": "LHLSVSRVELKPGDNLNVNFHLRTDAGQEAKIRYYTYLVMNKGKLLKAGRQVREPGQDLVVLSLPITPEFIPSFRLVAYYTLIGANGQREVVADSVWVDVKDSCVGTLVVKGDPRDNRQPAPGHQTTLRIEGNQGARVGLVAVDKGVFVR",
    "response#3": "LHLSVSRVELKPGDNLNVNFHLRTDAGQEAKIRYYTYLVMNKGKLLKAGRQVREPGQDLVVLSLPITPEFIPSFRLVAYYTLIGANGQREVVADSVWVDVKDSCVGTLVVKGDPRDNRQPAPGHQTTLRIEGNQGARVGLVAVDKGVFVR"
  },
  {
    "reference": "MSTISDIKLEDYWISPRLGFLSDQPVLTELPNPYYRCWEQMISSVLPTDGWVLIRRQIDQMPLLSTKKLHTEPEWRRAYTLLTTLAQVYIWGEANPSDKLPRSVAVPLREVSEHLRISPCASFASFCLWNVAPVPGVVPTDPDAYDPDNLHIINSFTGTKDEEWFFVISASIEATGGRIIARMLEAVEAVHHDRIHVVNEFLNELASCIAEICHLLERMYEKCNPSVFCHRLRRFFSGSKAKEDAGLPRGVFYEGTDGGGDWLQYCGGSNAQSSLIQLFDIILGVKQSGPGIRRACTSFHQEMRQYMPGPHRDFLAQMEKLSNVRSYVLSHPRHSNLHNSYDQAVAALALLRQKHMIVASRLPHPLAIMASTISG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The heme binding site should be stable and able to withstand changes in environmental conditions.2. We are looking for a protein with broad substrate specificity for L-tryptophan + O2 = N-formyl-L-kynurenine.3. The designed protein should be optimized for heme binding, metal ion binding activity in order to improve tryptophan catabolic process to kynurenine.4. The protein should be optimized for activity in Secondary metabolite biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. For general function, the protein need meet that Indoleamine 2,3-dioxygenase; part of the gene cluster that mediates the biosynthesis of terreazepine,; The first step of terreazepine biosynthesis is catalyzed by the indoleamine 2,3- dioxygenase tpzB which produces N-formyl-kynurenine through the catabolism of tryptophan; TpzA produces a 2:1 mixture of S-R enantiomers, which suggests that the A2 domain accepts both D- and L- kynurenine; The two-module NRPS tpzA then utilizes anthranilate and kynurenine to assemble terreazepine; The second C domain (C2) catalyzes the final cyclization event between the aromatic amine of kynurenine and the tethered carbonyl carbon, yielding the final terreazepine product; The T3 domain may facilitate the interaction with downstream tailoring enzymes. The designed protein sequence is ",
    "response#1": "LATRQPGAQAVQSSKSALTYLELDDLSSRLAIHLQARYNLAPGAMIMLCATKDVWAVVAMLAINKTGACFVPCDASHPVSRRQTMAGKCQSELALVSPEHETLFQGIIKESFIISEATVTKLPQETRDSWTVGERFPVAPSTPAYCFFTSGSLGEPKGCLGTHSALAAVAHQVPALRMDTKSRVLQFAKFGFGISFIEIFCTLAAGGTVCIASEHERLNALDAAIRRMEVNWALITPTLAQSLSPEEIPTLRKLFLGGEAPNDDLISRWQSKASLFQVFGTTEMAGVTMVSSEITSTAQRKIVGFPANSRIWLVESTGKDSNDTRLAPIGAVAELLIEGPSLAEGYWGRPEATAETFGDDGWLRTGDLGFIADGELFVTGRLKELIVIAGRNIYPTDIERAAEAVPGVREVTVVGAGLVGCLAALAFADRGHKVALYDARPDLRSEAELKNASLRSINLAVSARGIEALRSVDTKMAERVLADIIPMYGRMIHDLQGGQHAQAYGLWGECINSIDRAQLNRTMLDVIEDNANITFFPEHKLTNISLSRKDKKYQRPTSTFETKEGEERVVESDYIIGADGAFSKTRDRLQRYVRMNYAQQYIDCVYLELKIPKADGPDPFSISPNHLHIWPRHKYMLIALANGDGSFTSTLFAPPALMEQVCESQNTFISFFKEQFPDAYELMGESQILESYENNPRSPLVSLKCSPYNHKGECLLVGDAAHCMVPFYGQGMNAGFEDIRVLMEILDEKKWNVEEAFNTYTERRHKDLVAIV",
    "response#2": "LATRQPGAQAVQSSKSALTYLELDDLSSRLAIHLQARYNLAPGAMIMLCATKDVWAVVAMLAINKTGACFVPCDASHPVSRRQTMAGKCQSELALVSPEHETLFQGIIKESFIISEATVTKLPQETRDSWTVGERFPVAPSTPAYCFFTSGSLGEPKGCLGTHSALAAVAHQVPALRMDTKSRVLQFAKFGFGISFIEIFCTLAAGGTVCIASEHERLNALDAAIRRMEVNWALITPTLAQSLSPEEIPTLRKLFLGGEAPNDDLISRWQSKASLFQVFGTTEMAGVTMVSSEITSTAQRKIVGFPANSRIWLVESTGKDSNDTRLAPIGAVAELLIEGPSLAEGYLATRQPGAQAVQSSKSALTYLELDDLSSRLAIHLQARYNLAPGAMIMLCATKDVWAVVAMLAINKTGACFVPCDASHPVSRRQTMAGKCQSELALVSPEHETLFQGIIKESFIISEATVTKLPQETRDSWTVGERFPVAPSTPAYCFFTSGSLGEPKGCLGTHSALAAVAHQVPALRMDTKSRVLQFAKFGFGISFIEIFCTLAAGGTVCIASEHERLNALDAAIRRMEVNWALITPTLAQSLSPEEIPTLRKLFLGGEAPNDDLISRWQSKASLFQVFGTTEMAGVTMVSSEITSTAQRKIVGFPANSRIWLVESTGKDSNDTRLAPIGAVAELLIEGPSLAEGYYQD",
    "response#3": "LATRQPGAQAVQSSKSALTYLELDDLSSRLAIHLQARYNLAPGAMIMLCATKDVWAVVAMLAINKTGACFVPCDASHPVSRRQTMAGKCQSELALVSPEHETLFQGIIKESFIISEATVTKLPQETRDSWTVGERFPVAPSTPAYCFFTSGSLGEPKGCLGTHSALAAVAHQVPALRMDTKSRVLQFAKFGFGISFIEIFCTLAAGGTVCIASEHERLNALDAAIRRMEVNWALITPTLAQSLSPEEIPTLRKLFLGGEAPNDDLISRWQSKASLFQVFGTTEMAGVTMVSSEITSTAQRKIVGFPANSRIWLVESTGKDSNDTRLAPIGAVAELLIEGPSLAEGYLGRPDLTASRFITDPFRPGERMYRTGDVARWLPDGNLEYLGRSDDQLKIRGQRIELGEIDRVLQALPDVEQAVTHAHTLTTGDNARQLVGYLVSQSGLPLDHSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRRALPAPELAAQAARGYEAPQGETETLLAQIWQEVLGVERVGRHDHFFELGGHSLLATQVVSRVRQTLQVELPLRALFEAPTLSDLAARIEQLQLARSQPALPLIR"
  },
  {
    "reference": "MVVLTPERMAEYDRYAINTLGIPSAVLMENAGRGTYRLAKERYFSGKEKVTIFCGRGNNGGDGFVIGRYALRDGFQTRVFLLCKASDLKGDAALNMGIYKSIGGKISECTDKTKGITDGIRDADIIIDAIFGTGLSKPVSGIEKAVIEDINISGKPVIAVDIPSGIDGKTGQPLGTAIRATHTFTYGYPKIGQILQPGAYHTGKLTVIDISMPSLIEGKLGFDGRVIEGAMVRGFLKKRLPWDHKGTFGHVAVIAGSPGKTGAACMTSQAALKIGAGLVTLLIPESLNSIVASKLSEVMTYPVRDRGTGYFHLSAYKEISDFVSDKDVIVMGPGLSQESDTTMLVRELYQNINKPFVIDADGINAFQGQTDIIKGSGRKAVFTPHPGELSRLMGVTPKDINNDRVGVAQRFVRHTGANLILKGARTVIVSDKEELFINPTGNPALAKGGSGDILTGFVGGLLSQGHAPIEASIIGTYLHGYIADTWVQNNTDMDLLAGDLVSGIGKALRDIRDGKDRVYIEKSL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must be optimized for NADHX epimerase activity, NADPHX epimerase activity.2. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..3. The protein should be able to bind AMP ligand in a variety of conditions4. The protein requires Mg(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "MAGHKYDRGHAVVVSGPMTATGAARMAAASALRTGAGLVTVASPPDALFVNANHLTAVMLTRADGPEGLAEVLADTRRNAVCIGPGLPPDVATRELVTAALASEAAAVLDAGALTAFADDADALAAAMGGRGAVLTPHAGEFARLFGRDELPRLGKLEAARRAAARVGATVVLKGADTVTAAPDGRAAISENAPPWLATAGAGDVLAGMITAFLAQGASPFEAAAMGVWLHGDLAQRVGPALIATDLIANLK",
    "response#2": "LTERLFGLLSAPAAELHKGRAGHVLVLGGSEGLTGAAQLCALAALRAGAGLVTVGCPRGVLAEVKAGMPDIMALGLGHGMAWTPDCMDSLIPTLGRYDAVVIGPGLGRNKEAADFLALFPKAFDQSFDLGWAVPCIYDADALYHLAQNPGIMKELPQDAVLTPHPGEMARLLGMGMGEVQADRATAAHRFLATHPQVLTLKGAGTLVGQGDLLHLSPIVAPNLAVGGSGDVLAGVIAAILARGVNPLHAACLGVYWHGLCGLALAETFPARGNLATEIAHALPHVLKEAR",
    "response#3": "GPGDMAICLRKRFKEAHKGDFGRVVVIGGNKEYHGAPVLSARGALGTGIDMVYLFVPEVLLNPIRCISPDFIVTGYSGEKLTQISLDEGNKIFKKADAAVIGPGLGDDSETIEAVLSFLNHWDKPVVVDADALRALAFDKKILRGKHAILTPHAGELRRLSGLESENREATVKKAAEEFNATLLLKGSTDIVASPRGRIKFNMTGNAGMTRGGTGDVLAGIAAGLLAQEAEPFYAACASAFINGYSGDRLLERKGYFFLASELSMEIPLAIKDVFP"
  },
  {
    "reference": "MIADVKKSAEQKMQKSLDALKVDFSKVRSGRPHTGLLDHIMVDYYGTPTPIKQLANVTLADARTIGIIPWDKKIFSAIEKAIRDSDLGLNPMTVSDMVRVPMPPLTEERRKDLTKIVKTEAEAARVAMRNIRRDANAHLKELLKDKLIAEDEDRRAQDEIQKLTDRYIAEVDKLLQTKEAELMAV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis.2. The designed protein localizes to the cytoplasm. The designed protein sequence is ",
    "response#1": "EKGLLRVRTGRASLSMLDGVKLDYYGTPTPINQAATVAIPESRLITIQPWDASVIKDIEKAILKSDLGLNPSNDGKIIRIAIPPLTEDRRKEMVKLVNKMSEEGKISVRNIRRDANETIKSMKKDGDIAEDLAFKGQDEVQKITDDFVKKVDDICAQKEKEIE",
    "response#2": "EKGLLRVRTGRASLSMLDGVKLDYYGTPTPINQAATVAIPESRLITIQPWDASVIKDIEKAILKSDLGLNPSNDGKIIRIAIPPLTEDRRKEMVKLVNKMSEEGKISVRNIRRDANETIKSMKKDGDIAEDLAFKGQDEVQKITDDFVKKVDDICAQKEKEIL",
    "response#3": "EKGLLRVRTGRASLSMLDGVKLDYYGTPTPINQAATVAIPESRLITIQPWDASVIKDIEKAILKSDLGLNPSNDGKIIRIAIPPLTEDRRKEMVKLVNKMSEEGKISVRNIRRDANETIKSMKKDGDIAEDLAFKGQDEVQKITDDFVKKVDDICAQKEKEIL"
  },
  {
    "reference": "MTNLLFVALGGSIGAVLRYLMSIIMIQLFGSSFPFGTLLVNVLGSFFMGIVYALGQVSHVSPELKALVGVGLLGALTTFSTFSNETLLLMQQGYWFKSLINVLLNVSLCIFMVYLGQQLVFSRV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein localizes to the plasma membrane.2. For general function, the protein need meet that Important for reducing fluoride concentration in the cell, thus reducing its toxicity.3. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "MPIS",
    "response#2": "MVRKS",
    "response#3": "MPPSGPPQGT"
  },
  {
    "reference": "MRQLLLATRNAHKTREFAEILGDEFHVSDLLEAQNIPEIKETGRTFAENAIAKAVDLSRAVVNLVVGEDSGLEVDALGGAPGIRSARYAGENATARDNIDKLLRALARTDPDRMRRSARFRCVLALACGGKLLGTFEGLIGGIIVDPPRGENGFGYDPLFAPDGFDKTFAELPAKVKNRISHRAQAIRALRARLPALPRGDQGLAGGGGGPGG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have a high specificity for Mg(2+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "LQLLSRKGIGLPRTGFASKPDKIQDLIKNVGGAPLVIKLLEGTQGIGVVLAETNKAAESVIEAFMGLKANILVQEFIKEAGGADIRCFVVGDKVVAAMKRQAAEGEFRSNLHRGGVAQLVRLSKDERATALNAAKAMGLNLCGVDILQSNNGPVVMEVNSSPGLEGIEQATGKDVAGLIYEFIK",
    "response#2": "MVAHMNEEALRLTLETGIAHYWSRSRKTLWKKGETSGNLQSVVELRTDCDQDALWLKVHVAGDGPTCHTGRRSCFG",
    "response#3": "MVAHMNEEALRLTLETGIAHYWSRSRKTLWKKGETSGNLQSVVELRTDCDQDALWLKVHVAGDGPTCHTGRRSCFL"
  },
  {
    "reference": "AAGRRSLKTNLCKWCDATAPACDERVCYSNCNLNSYCESPYEICVAIWRQDNESIRISTLCHNPQHPIDNLMVPNYNTSRCIMSHQPSEDGVIYICGCVDEQECNDKLIFENHTNGYSMLQSKEVIPVAAISLLPPLLVAVMVSVIFYLCRTQKRRKRAKAWGQKHVRHPALPERGKAAERDEKLSVMTDDNQAAMSSTCANSINHNTELLPIELDEMVGKGQFAEVWRAKLNHASSGQYETVAVKIFPCEEYSSWKNESQIFTDASLKHDSVLQFLTAEDRGAGPRREYWLITAYHSRGNLKDYLSRHILSWMDLQKMAGSLVNGVAHLHSDYTACGRPKIPIAHRDIKSTNILVKNEQECVLCDFGIAIRLDPSLTADDFANSGQASTTRPPALRLLVGTARYMAPEVLESRVNLEDLESFKQMDVYSMALVLWEMASRCEVVGEVRNYELPFGSKVQEQPCVDTMRDIVLHGRGRPEIPSSWLVHQGMRFLCDTITECWDHDPEARLTAHCVAERLSLMAQMGCDDILNNNTESEGGKAEPGGGARR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have a binding site that is specific to the target ATP ligand.2. The presence of Protein kinase domains is critical for the interaction of the designed protein with its intended targets.3. The protein should contain a Helical membrane-spanning region.4. A protein that can perform transforming growth factor beta receptor activity, type II, metal ion binding in plasma membrane, receptor complex to support apoptotic process, anatomical structure morphogenesis, tissue development.5. The protein design should incorporate a binding pocket that specifically recognizes Mn(2+) to enhance enzymatic efficiency.6. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for ATP + L-threonyl-[receptor-protein] = ADP + H(+) + O-phospho-   L-threonyl-[receptor-protein]. The designed protein sequence is ",
    "response#1": "IELDVQVGKGRFAEVYKAKLKQGSSVSEEQGFETVAIKIFPCEEYTSWKNEKDIFSDADLRHENVLHFLTAEERKVQASGRRQYWLITAYQPRGNLQEYLTHHVINWHDLWLLGSSLARGVAHLHSDRTPCGRYKVPIAHRDIKSSNILVKSDLTCCLCDFGLALRLDNSLSVDELANSGQVGTARYMAPEVLESRINLENIESFKQADVYSMALVLWEIISRCSAIGEVKEYEPPFGNLREHPCVESMKDSVLRDRGRPEIPNSWLNHTGVQVVCASIEECWDHDPEARLTAQCVAERFIELDTLVGKGRFAEVYKAKLKQNTSEQYETVAVKIFPYEEYASWKTEKDIFSDVNLKHENILQFLTAEERKTEIGKQYWLITAFHARGNLQEYLTRHIISWEDLWKLGGSLARGIAHLHSDHTPCGRPKTPIVHRDLKSSNILVKNDLTCCLCDFGLSLRLDPSLSVDDLANSGQVGTARYMAPEVLESRMNLENMESFKQTDVYSMALVLWEMTSRCNGVGEVKEYEPPFGSKVREHPCVESMKDNVLRDRGRPEIPSSWLNHQGIQMLCETLIECWDHDPEARLTAQCVAERFA",
    "response#2": "LCRFCDVKVTTCSNKGQCNSNCNITAICENDGEVCVAVWRQNDENVTLETICHDPKKPLYGYHLEDYNSKECSMREKKAEGGLMYMCSCKEEECNDVPIFSDDTQYRTGPWAKTRFTGSPQMDESYTSPFILTQDVNGSGYFSFVERGKPSRMILTSEGTMKVLVHNGMDWESTYEGPANSCDIYGVCGPFGLCVVSIPPKCKCFKGFVPKC",
    "response#3": "FAGITKESPFSIGQTLSSSNGVYELGFFSLNNSQNQYLGIWFKSIIPQVVVWVANREKPVTDSAANLGISSNGSLLLSNGKHGVVWSTGDIFASNGSRAELTDHGNLVFIDKVSGRTLWQSTGGGGGSTSGGGTSTTTTYRTGPWAKTRFTGSPQMDESYTSPFILTQDVNGSGYFSFVERGKPSRMILTSEGTMKVLVHNGMDWESTYEGPANSCDIYGVCGPFGLCVVSIPPKCKCFKGFVPKT"
  },
  {
    "reference": "MSRIGKTPVLIPEKVTVDLDGLIVTVKGPKGELKRLMPEGVSFDKKDNTVVVSPTTNKIHSRQRHGLCRALIANMVEGVTQGFSKKLEIVGVGSRAQVKGKNLVVSAGYSHPVEMIPPDGITYKVESNTNVTVSGIDKEIVGNEAAKIRSIRPPEPYKGKGIKYHDERILRKAGKSGKK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that This protein binds to the 23S rRNA, and is important in its secondary structure; It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.2. The protein should be optimized for ribosome, ribonucleoprotein complex-specific folding and stability, while maintaining rRNA binding, structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#2": "RRSVTKKRVVPPDAVYNNRLLSMTIRRVMKSGKKSLAARIVYDALDIIKERTGSDPIEVFETAIRNLTPLVEVKARRVGGATYQVPMEVRQGRGTALALRWLIGYARQRSGKSMAIKLANELMDAANETGGAIKKREDTHRMAEANR",
    "response#3": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG"
  },
  {
    "reference": "MARVKGGTVTRARRKKWIKLAKGYFGHKSVGYKVAKQAVVKSWTYAFRDRKQVKRNFRKLWIARINAAVRPHGLSYSQFINGLKKAEVLINRKMLSELAINQPQTFEKLVKLVQGK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that It is not involved in the protein synthesizing functions of that subunit.; Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit2. The protein design must be able to perform structural constituent of ribosome, rRNA binding in order to contribute to translation. The designed protein sequence is ",
    "response#1": "MPLPIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#2": "GPIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#3": "MQRLTKISGQKPLITKARKR"
  },
  {
    "reference": "MIACRMSSQDLSITAKLINGGIAGLVGVTCVFPIDLAKTRLQNQQGKDVYKGMTDCLVKTARAEGFLGMYRGAAVNLTLVTPEKAIKLAANDFLRQLLMQDGTQRNLKMEMLAGCGAGICQVVITCPMEMLKIQLQDAGRLAVCQQASASATPTSRPYSTGSTSTHRRPSATLIAWELLRTQGLSGLYRGLGATLLRDIPFSIIYFPLFANLNQLGVSELTGKASFTHSFVAGCAAGSVSAVAVTPLDVLKTRIQTLKKGLGEDTYRGVTDCARKLWTQEGAAAFMKGAGCRALVIAPLFGIAQGVYFIGIGERILKCFE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be designed to have high stability and activity towards H(+)(in) + L-glutamate(in) = H(+)(out) + L-glutamate(out).2. The designed protein must possess L-glutamate transmembrane transporter activity, L-aspartate transmembrane transporter activity, amino acid:proton symporter activity, transmembrane transporter activity to support L-glutamate transmembrane transport, malate-aspartate shuttle under certain conditions.3. The protein should contain a Helical; Name=1, Helical; Name=2, Helical; Name=3, Helical; Name=4, Helical; Name=5 membrane-spanning region. The designed protein sequence is ",
    "response#1": "RKDLALEMATAARKAWSQLAQSPLQKRNHALELFSQKISSSAKGLLEANAIDVEGAKKKNVIAPVIDRLTLNEKRIAALSESVKKTIANDLQELDHQQHSWTLENGLKVTRKQVPFGVLFFIFEGRPNVAVDACALAIKTGNAVLLKGSRSATNSNKFMVKLARQALGEAGLPEDSVQLFEGEQEDFLRLLRMEKLIDIVVPRGGEEFIEFVKKNSSVPLLYAGGGVCHVYVDKDADDEKAVKIVINAKTQRPGVCNAAEVLLVHEHKAKTLLPKIA",
    "response#2": "EMATAARKAWSQLAQSPLQKRNHALELFSQKISSSAKGLLEANAIDVEGAKKKNVIAPVIDRLTLNEKRIAALSESVKKTIANDLQELDHQQHSWTLENGLKVTRKQVPFGVLFFIFEGRPNVAVDACALAIKTGNAVLLKGSRSATNSNKFMVKLARQALGEAGLPEDSVQLFEGEQEDFLRLLRMEKLIDIVVPRGGEEFIEFVKKNSSVPLLYAGGGVCHVYVDKDADDEKAVKIVINAKTQRPGVCNAAEVLLVHEHKAKTLLPKIAGGLLNRKVELRCDDKSLAILSKEGFEVKKAGETDFDTEFGDLIMAVKIVASTEEAIAHINQHGTHHSDSIVTENPPMAQAFLREVDSACVYWNASTRFTDSEVFGFGPELCISTQKLHSRGPLSLKHLFTNKYEVEP",
    "response#3": "EMATAARKAWSQLAQSPLQKRNHALELFSQKISSSAKGLLEANAIDVEGAKKKNVIAPVIDRLTLNEKRIAALSESVKKTIANDLQELDHQQHSWTLENGLKVTRKQVPFGVLFFIFEGRPNVAVDACALAIKTGNAVLLKGSRSATNSNKFMVKLARQALGEAGLPEDSVQLFEGEQEDFLRLLRMEKLIDIVVPRGGEEFIEFVKKNSSVPLLYAGGGVCHVYVDKDADDEKAVKIVINAKTQRPGVCNAAEVLLVHEHKAKTLLPKIAGGLLNRKVELRCDDKSLAILSKEGFEVKKAGETDFDTEFGDLIMAVKIVASTEEAIAHINQHGTHHSDSIVTENPPMAQAFLREVDSACVYWNASTRFTDSEVFGFGPELCISTQKLHSRGPLSLKHLFTNKYEVEP"
  },
  {
    "reference": "MNRSRAREVSAVKKPVNSDQQGKVAGLAVVVMAAGLGKRMRSKQAKVLHRVAGQEMVLYSVRLGLRLAGDRVAVVVGHQADLVRQAIVRATSGKSDGSPVAIVEQQEQLGTGHAVLQSRPVFAVGKRGAPSRYLILNGDTPLLQEKTVRELLRVHEAERATVTILTAMLEDASGYGRVVRAHSVERGVSRIVEDRDANTEERAIHEINVGTYVVDGEFLFNALEKLDPSNAQGEYYLTEIVHLAAEQGRRVAAVTLDNPDEGLGVNTRRQLAEAERVVRQQIRDRWLDAGVTMIDPASTWIDATVTIGKDTVLYPNVTLEGTTVIGEDCVLRSSTRLTNCTLGNGVEILDHCVFADSQIEDSAHLGPFVHLRPGVVVRKRAKVGNFVEMKKTDLGEGSKANHLSYLGDTTIGKGVNIGAGTITCNYDGVRKYQTVIGDGVFLGSDTQLIAPVTVGAGAIIAAGTTVTEDVPADALVIGRVPQVNRAGWATRRRALQASVNRETTSTRLTPYASRLTKKQPLVSKAKPVKNRSRS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should contain one or more MobA-like NTP transferase domains that are essential for its function.2. The protein design must be focused on enhancing the efficiency of peptidoglycan biosynthetic process, cell morphogenesis.3. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.. The designed protein sequence is ",
    "response#1": "VVVLAAGQGKRMHSRLPKVLQPLAGAPLLAHVLDAARSLSPARIAIVHGHGGERVPQAFADAPGLAWVRQEPQLGTGDAVRQALPLLDGAGMVLVLYGDVPLIGAATLARLARACSGARLVLLTVTLPDPTGYGRIVRDAQGQVLAIVEHKDASPAQRAIQEVNTGILAAPTKALRQWLTALDNTNAQQELYLTDIVALAVKDGVPVVSAQPAAVWETLGVNSKVQLAELERVYQQQAARALLLQGVSMLDPHQVWVGPEVCIGQDCELLPQTFLWGSTRIGSDCVIGPQSRLTNATVGNGCIVDETVIVDSCIDDGVVCGPRAYIRGNAHLKHNAKAGTHVEIKGSEIGERSKVPHLSYIGDARLGSDVNIGGGSITCNYDGKHKSHTEIGNHVFIGSDTMMVAPVTIGDNALIGASSCITKDVPAGSLAIERSSQRNIAGWQRPVKK",
    "response#2": "SATPKVLHTLAGRSMVGHALAAARALEPERVAVVVRHERERVAAHVGEVDPAALLVDQDDVPGTGRAVQVAMSALDAAAQAAVADAASDAAADHDAHGGSAADALLEGAVVVLAGDVPLLDAGTLTELLVAHTEDGNAVTVLTTEVPDPTGYGRIVRDADGNVAAIVEERDADEAQRAIREVNAGVYVFRAGELRRALGQVGTDNAQGEKYLTDVVALLRAAGHRVGAAPAHDPLQTEGVNDRAQLAELGRVLNRRIVERWQRATRIGSKASIGANTSLVAPVKVGDHSVTGAGSTIEDAVIGPSCQIGPYARLRPGARLAEEVHVGNFVEIKGVTLIDPDSITIDDTVILHPDTIVEPQTHLRGNTEIGSGCRIGPGSLIENSQIGENVTVMYSVVSNSHVEAGCRIGPYTHLRGEVRIGESCRIGNFVEIKKATIGNKTNVAHLSYIGDATLGDRVNVGAGTITANYDGVKKHHTHIGSGTKTGANSVFIAPVTIGEDVTVAAGSVITKNVPNEALAIARENLTVGEGTVVR",
    "response#3": "AVILAAGRGTRMRSSLPKVLHPLLGVPMLDHVFQHLREAGASDVIVVVGHGSDQIREHLRDADVRVAIQDPQRGTGHALLEALDQCPPRNQTVVVVNGDLPDLDSALVKRLVEQQVSNSAALTVTCGKVDHPQGMGYGRIVRDAAGNVQRIVEQKDATPAERAICEINTGIMVAPTAQLKRWLGGLRNDNAQGEYYLTDIVAMAVEDGVPVHTAQPASEWEVAGVNSKVQLAELERIHQQNLANGLLEAGVTIVDPATTWIDAGVTLEPDVVLHPGTQLKGTTSVATGAQIGPWCTLTDTVVGEGAVVRNAVCEQAVIGPEASVGPFAYLRPGTVLGRKGKIGTYVETKNAKIGEGSKVPHLTYVGDATIGVGSNIGASTVFVNYDGVNKHHTVVGDHVRVGSDTMLVAPVTIGDGAYTAAGSVITQDVPPGAMAVARSRQRTIQGWQRPVKKK"
  },
  {
    "reference": "MLTDILKKIFGSQNERILKRIAPLVDEINSYEPVMRKLSDAALKAKTPEFKQRIANGEPLDDLLPEAFAVAREGAVRTLGMRPFDVQMIGGIVLHEGMIAEMKTGEGKTLVAVMPIYLNALTGRGVHLVTVNDYLARRDSEWMGQVYKFLGLSVGCIVHGLDDPERKEAYGADVTYGTNNEYGFDYLRDNMKFRIEDMVQRELNYAIVDEVDSILIDEARTPLIISGPAEKSTALYYNINRIIPQLKPETHYTKEEKSRTVALTEDGVTRTEKLLGVDNLYDPRQIDILHHVQQALRAHVLFKRDVDYIVKDGKVIIVDEFTGRLMPGRRYSEGLHQALEAKENVHIENENQTLASITFQNYFRMFDKLAGMTGTAETEAAEFAKIYKLEVVVIPTHRKMIREDYADCIYRTEAEKFRAVAEEIKEAYAVKRPVLVGTVNIAKSEKLSGILKRQGVPHQVLNAKHHEKEAEIVALAGQPGAVTISTNMAGRGTDIVLGPGVVDVGGLHIIGTERHEARRIDNQLRGRSGRQGDPGSSRFYLSLEDDLMRIFAADRLSGLMQRIGMKEDEPIEHRLITKAIENAQSKVEAQNFSIRKQLLEYDDVMNQQREVIYRQRREALQGENLKPVVLDMIEDLLEGILAETADEKHYAEDWDLEKINSEVLRLFGLQMNLTVESLGDIEYEEFRDSLLERLTKRYEAREQEFGESMMRELESYLLLQTVDTYWKDHLLNMDHLKEGIGLRGYGQQDPLIAYKREGHALFDEMIER",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Interacts with the SecYEG preprotein conducting channel The designed protein sequence is ",
    "response#1": "LDDNGRRRLADLTAQGNENSFAIVVDNQVVSAPTVSGPLDTSELQIEGAFDLQAANNMAVVLRSGALPQAVTVLEERTIASALGEDYASAAVLAALLAALVVGLFMVLSYGILGVIALVALVVNIVILTAVLSLIGASISLASIAGLVLTIGLAVDAHILIYERVREDRRKGYSVVQAMESGFYRALSTIVDANLTTLIAALVLFLLGSGTVHGFALTVAIGIGTTLFTTL",
    "response#2": "LDDNGRRRLADLTAQGNENSFAIVVDNQVVSAPTVSGPLDTSELQIEGAFDLQAANNMAVVLRSGALPQAVTVLEERTIASALGEDYASAAVLAALLAALVVGLFMVLSYGILGVIALVALVVNIVILTAVLSLIGASISLASIAGLVLTIGLAVDAHILIYERVREDRRKGYSVVQAMESGFYRALSTIVDANLTTLIAALVLFLLGSGTVHGFALTVAIGIGTTLFTTLF",
    "response#3": "SSELYIAINKLIPSLVEQEKEDSDEFQGSGDYSLDLKNKQANLTERGLEKCENWLVEQGLMRPEDSLYSASNLGLFHHISAALRAHTLFQRDVDYVVKDGEIVIVDEHTGRTMAGRRWSDGLHQAIL"
  },
  {
    "reference": "MRVNDNQPFDLVTNYQPAGDQPQAIKKLVSGIEQGNRNQLLLGVTGSGKTYTMANVIAQTQRPTIVMAHNKTLAAQLYGEFKAFFPNNAVEYFVSYYDYYQPEAYVPSSDTFIEKDAAINDHIDQMRLSATRALLERRDAIIVASVSAIYGLGDPEAYMKMLLHVVEGDRINRDDLIRRLVEMQYTRNELEFLRGTYRIRGEILDVFPAESDQFAIRIELFDDEVDSIRWFDPLTGKMQRKVPRVTIYPKSHYVTPKDNLSRAIETIKDELQDQLKFFREHDKLLEAQRIEQRTRYDLEMMQQLGYTNGIENYSRHLSGRSPGAAPPTLFDYIPEDALLIIDESHVTVPQIGAMYKGDRSRKENLVNYGFRLPSALDNRPMKFEEWERIVPTTVFVSATPAKYELEKSDQIVEQVVRPTGLIDPEIEIRPVLTQVDDVLSEINIRKEMDERVLITTLTKRMAEDLTSYLKEYGVKVAYLHSDIDTVERVKIIHELRTGVYDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNIKGKAILYADRMTDSMQKAIDETERRREKQIEFNKIHGITPRSAVRQKVKEIDTGEVFNDDDIEGKISEQARALSADERHILADPKLFAKHMSKLEKEMLKASKELQFEQAARLRDEILRLKAQMLH",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The presence of UVR, Helicase ATP-binding domains is critical for the interaction of the designed protein with its intended targets.2. For general function, the protein need meet that If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed; Then UvrB probes one DNA strand for the presence of a lesion; The UvrABC repair system catalyzes the recognition and processing of DNA lesions; If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.; A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities; Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer; DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands3. The ATP binding site should be located in a region of the protein that is accessible to the ligand.4. A protein that contributes to SOS response, nucleotide-excision repair by functioning in excinuclease repair complex, cytoplasm with ATP hydrolysis activity, ATP binding, excinuclease ABC activity.5. The protein's functional design necessitates the inclusion of a Beta-hairpin motif. The designed protein sequence is ",
    "response#1": "SDYRPLGDQPKAIRSLVNGIKAGMREQTLLGVTGSGKTFTVANVIAEVQKPTLVISHNKTLAAQLYEEFREFFPENAVEYFVSYYDFYQPEAYIPQTDTYIDKEASINDEIDRMRHSATQSLLSRDDVIVVSSVSCIYGIGAPTDYGEFTLHLEVGSGPGREEVLEGLINMQYERNDVEFDRGQFRVRGDTVEINPIHGTPPIRIEFFGDEIDSISTVHRVTGRRIQKLDRVTIFPAKHFVIPEDRLQRAIESIEAELEERLTELRSQNKLLEAQRLEQRTRFDMEMLREMGYCQGIENYSMHLSGRKWGEKPNTLLDYFPEDFLTVIDESHVTVPQIRGMYNGDRARKDTLVEYGFRLPSARENRPLRFDEFQESVNQVIYVSATPGRYELSRSQNIVEQIIRPTGLVDPEVRISPYKPLGDQPKAINSLVDGINKGEKEQTLLGVTGSGKTFTMANVIEKVQKPTLVISHNKTLAAQLYEEFKEFFPDNAVEYFVSYYDYYQPEAYVPRTDTFIDKESSVNEEIDIMRHSATQSLLSRDDVIVVSSVSCIYGIGSPEDYGEFAFGIAVGDNYDRSDIIRKLVFMQYERNDIEFARGHFRVRGDVIEINPVHGTPPVRVELFGDEIDAISLIDKVTGKKTESLKRYMIFPAKHFVVGQDKMDTAIRNISDELDERLNEFNLSNKLLEAQRLEQRTRFDIEMLQEMGYCPGVENYSMHLSGRKWGEKPYSLLKYFPEDYLTIIDESHVTLPQIRGMYNGDRARKETLVEHGFRLPSAKENRPLRFDEFESSINQIIYVSATPGAYELSRSSNIVEQIIRPTGLVDPEVII",
    "response#2": "SEYQPSGDQPKAIAELAGRITAGETDVVLLGATGTGKSATTAWLVEAVQRPTLVLAHNKTLAAQLANEFRELLPNNAVEYFVSYYDYYQPEAYVPQTDTFIEKDSSINAEVERLRHSTTNSLLSRRDVVVVSTVSCIYGLGAAEEYLEAMVALQVGQNVGRDQLIRRFVAMQYERNDIDFSRGKFRVRGDTIEIIPVYEEHALRIELFGDEIEALYSLHPLTGEVLARTDAVSVFPATHYAASPATIQRAIGTIQTELHDRLVELEREGKLLEAQRLRMRTQFDIEMMEQIGFCSGIENYSRHIDGRAPGEAPHCLLDYFPDDFLVVIDESHVTVPQIGAMYEGDASRKRTLVEHGFRLPSAMDNRPLRWNEFKERVGQTVYLSATPGQYELGIADGVVEQIIRPTGLVDPQILVSPYKPLGDQPKAINSLVDGINKGEKEQTLLGVTGSGKTFTMANVIEKVQKPTLVISHNKTLAAQLYEEFKEFFPDNAVEYFVSYYDYYQPEAYVPRTDTFIDKESSVNEEIDIMRHSATQSLLSRDDVIVVSSVSCIYGIGSPEDYGEFAFGIAVGDNYDRSDIIRKLVFMQYERNDIEFARGHFRVRGDVIEINPVHGTPPVRVELFGDEIDAISLIDKVTGKKTESLKRYMIFPAKHFVVGQDKMDTAIRNISDELDERLNEFNLSNKLLEAQRLEQRTRFDIEMLQEMGYCPGVENYSMHLSGRKWGEKPYSLLKYFPEDYLTIIDESHVTLPQIRGMYNGDRARKETLVEHGFRLPSAKENRPLRFDEFESSINQIIYVSATPGAYELSRSSNIVEQIIRPTGLVDPEVII",
    "response#3": "APFRPTGDQPTAIASLIESLQGEHKFQTLLGATGTGKTFTMASVIAELGRPTLVLAHNKTLAAQLCNELRQFFPENAVEYFISYYDYYQPEAYIPVTDTYIEKTASINDEIDMLRHSATRSLFERRDVIVVASISCIYGLGIPSQYLKAAVPLQVGAEYDPRLVIRDLVNVQYSRNDVELQRGRFRLKGDVLEIVPAYEDRVIRIEFFGDEVEALRLIDPVSGEILQSLDRISIYPARHFVTPEETLEKACQQIQTEMEQQVAFLEKNNLLVEAQRLSQRTRYDLEMLREVGYCNGVENYSRYLADRQAGEPPECLVDYFPEDWLLVIDESHVTIPQLRGMYNGDQARKKVLIDHGFRLPSAADNRPLKAEEFWQKVKQCVFVSATPGVWEIEQSEARVIEQVIRPTGVLDPEIFVSDYRPLGDQPKAIRSLVNGIKAGMREQTLLGVTGSGKTFTVANVIAEVQKPTLVISHNKTLAAQLYEEFREFFPENAVEYFVSYYDFYQPEAYIPQTDTYIDKEASINDEIDRMRHSATQSLLSRDDVIVVSSVSCIYGIGAPTDYGEFTLHLEVGSGPGREEVLEGLINMQYERNDVEFDRGQFRVRGDTVEINPIHGTPPIRIEFFGDEIDSISTVHRVTGRRIQKLDRVTIFPAKHFVIPEDRLQRAIESIEAELEERLTELRSQNKLLEAQRLEQRTRFDMEMLREMGYCQGIENYSMHLSGRKWGEKPNTLLDYFPEDFLTVIDESHVTVPQIRGMYNGDRARKDTLVEYGFRLPSARENRPLRFDEFQESVNQVIYVSATPGRYELSRSQNIVEQIIRPTGLVDPEVRI"
  },
  {
    "reference": "MTAVAASSRVSDPLAASSSAHPRTLRSSPRKRTTSHDSSISPSAAQSKQLPRRKKPRTELVDESELDCAACPAVGQPAPTPGKGAASDRETWICCTHCKTWFHCICIGLENPDDFSKWYCQPCITRSEQTFESGTSSSHPPFANVVRPPRRKSERAKLQIDYAAIQEGIPADPLGRWKNLLNAYEFEPDQFRRMQGHEWTFDWLLHDESALKQPVLVPAPPDRSSQAPHVQAKAEDVAASPVPRPKPARAKKQATHRLVPCHTSIPGMVVPPPEMSIFDVADIIGHDTPVEVIDVASQSSSKASWTISEWAEYFNTPKEKKKKTLNVISLEVTGTPMQAYVEAPQLVRDLDWVTRDWPAERRDASCSENSWPKVQRYVLMGVEGAYSDWHIDFAGSSVYYHVIWGQKTFLFAPPTARNLAAYKAWCSSTRQDFDWLGDHLHSLTRVDIGPGETMLIPSGWLHCVYTPKNTLVVGGNFLTDWNVATQWKLVEIEEATKVPRKFRFPHLKRLSWFVAKGWNDRLEPLAEFETLTKEEDQVLEESAQVSAQVDVGSLTEVVPPLKVLNNIELVLQSLSDDLELIQDPYVAESGDERKVKQQKAAREAIPTHHVGNIQKAEAMLASLRQRVDRAKSLADAVQSERVCLTWEATRAKKAKAANGSAIKSRKARR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Fe cation binding.2. The designed protein should have metal ion binding within nucleus to regulate chromatin organization.3. The protein must be engineered to have a high affinity for Fe(2+) in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "SLDHIATCIKNGEMNYFVEWYRKCLGFKLLSDKDSVATDESENFENELILDKNFYVITKSDFKYTKKENIGLKMAVLSNQPLNTSFHKTPPIQFVISEAIQGGEGQIEQFIHYFGGEGVQHLAFNSNNIFKAVEIAKSQKLEFVYIPQSYYSKLDERLKHLFPNSIPKILKENLMKFGID",
    "response#2": "LDFTLQPGAQIHQNVPESWNAFAYILESGEGGGVFSSSNSSPIPAHSVVVFGPGNDGVSVWNKSSSKQLRFVLIAGEPIGEPVVQYGPFVMNTQAEIDMTIEDYHYGKNC",
    "response#3": "LDFTLQPGAQIHQNVPESWNAFAYILESGEGGGVFSSSNSSPIPAHSVVVFGPGNDGVSVWNKSSSKQLRFVLIAGEPIGEPVVQYGPFVMNTQAEIDMTIEDYHYGKNA"
  },
  {
    "reference": "MRVRGMQRNWQHLGKWGLLFLGILIICNAKDNAKDNLWVTVYYGVPVWRDADTTLFCASDAKAHDTEVHNVWATHACVPTDPNPQEVHMENVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLKCSNATFNATTNATVELVKNCSFNVTTELRDKKKNVYALFYKVDIVPIGGNSNEYRLINCNTSAITQACPKVTFDPIPIHYCAPAGFAILRCNDKTFNGTGPCTNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENITNNAKTIIVHLKEPVEINCTRPSNNTRTSVSIGPGQAFYRIGDIVGDIRKAFCVINETQWNNTLRQVAGKLRKHFNKTIKFQPHSGGDLEVTMHSFNCRGEFFYCNTTKLFDSIWNETTAQGNSSSTNKTITLPCKIKQIINMWQGVGKAMYAPPISGVLNCTSNITGILLTRDGGNSSSTEEIFRPEGGNMKDNWRSELYKYKVVQIEPLGLAPTRARRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASITLTVQARQLLTGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLALERYVKDQQFLGIWGCSGKTICTTNVPWNASWSNKSYEEIWGNMTWVEWDRQISKYSEQIYELLTESQNQQEINEKDLLALEEWASLWNWFSITRWLWYTKIFIMVVGGLIGLRIVFAILSIVNRVRKGYSPLSLQTLIPRSREPDRPEGIEEGGGEQDRDRSIRLVNGFLAIVWDDLRNLCLFSYHLLRDFILIA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a specific function in fusion of virus membrane with host plasma membrane and should be localized to viral envelope, plasma membrane in order to carry out this function efficiently.2. The designed protein should contain one or more Retroviral envelope protein GP41-like domains that are essential for its function.3. The protein should have a Helical domain that is long enough to span the entire membrane.4. For general function, the protein need meet that During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Transmembrane protein gp41: Acts as a class I viral fusion protein5. The protein design must feature the YXXL motif; contains endocytosis signal motif. The designed protein sequence is ",
    "response#1": "MRVKGTRKNYWHLWRWGTMLLGMLMICSATEQLWVTVYYGVPVWRDAKTXLFCASDAKAYDKEVHNVWATHACVPTDPNPQEMDLXNVTENFNMWENNMVEQMQTDIXSLWDQSLKPCVKLTPLCVTLICTNIXVNNTSCNSXXSNSTGWGKEEIKNCSFNITTELRDKRQKVYSLFYRLDVVPIEEGQGNSSNSGYKEYRLINCNTSAITQACPKVTFNPIPIHYCTPAGYAILKCNEETFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVIIRSENITNNVKNIIVQLAEPVRINCTRPNNNTRTSIRIGPGQTFYTSNIIGDIRQAHCNVSRAEWNKALNKIGKQLRKYFVNKTIKFANSSGGDLEITTHSFNCEGEFFYCNTSGLFNGTWNASMQESNSTESNETIILPCRIKQIINMWQRVGQAMYAPPIQGIIKCVSNITGLILTRDGKSSNSTDEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRAVGREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNASWSNKSQKEIWENMTWLQWDKEISNYTDIIYHLIKESQNQQEKNEQDLLALDKWADLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSVINRVRQGYSPLSFR",
    "response#2": "MRVRGIQRNWPPWWIWGILGFWMVLICRGVGNLWVTVYYGVPVWKDAETTLFCASDAKAYETEKHNVWATHACVPTDPNPQEIHLENVTEDFNMWKNSMVEQMHTDIISLWDQSLKPCVKLTPLCVTLNCSNVNGNSSFSNNVNNSDSSNNTISEMKRCSFNTTTVLRDKKKTQYALFYKLDIVPLENSTSEEYRLINCNTSAVTQACPKVNFDPIPIHYCAPAGYAILKCNNKTFNGTGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEGIIIRSENLTNNAKTIIVHLNESVEIECTRPSNNKRTSIRIGPGQAFFATGEVTGDIRQAHCNISTGKWKKTLQKVSKKLEEHFHNKIIKFNSSSGGDLEITMHSFNCRGEFFYCNTSRLFTGNYTSNGTSEESGSESIITLPCRIKQIINMWQEVGRAMYAPPIEGNITCKSNITGILLVRDGGDNTSETFRPGGGNMKDNWRSELYKYKVVEIKPLGIAPTKAKRRVVERREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNASWSNKSQKEIWENMTWLQWDKEISNYTDIIYHLIKESQNQQEKNEQDLLALDKWADLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSVINRVRQGYSPLSFRLIPNPRGPDRLGGIEEEGGEQD",
    "response#3": "MRVRGIQRNYQQWWIWGILGFWMLMICNVFGNLWVTVYYGVPVWKDANTTLFCASDAKAYSTEKHNIWATHSCVPTDPNPQEILLENVTENFNMWKNGMVEQMHEDIISLWDESLKPCVMLTPLCVTLNCTEANITKANSTGGNSTNGTSSTNHSIVNEIGEVKNCSFNVTTEIIDRKQQEYALFYRLDIVPINNSTTEYRLINCNVTTIKQACPKASFEPIPIHYCAPAGFAILKCNDKEFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEGGIRIRSENFTDNTKTIIVQLNSSVEINCTRPGKTQKQIRIMHIGPGRAFHVNKEIGGMRQAHCNVSKEKWGRMLNQVATQLEEKYNRSIFFNSSSGGDIEITTHSFNCGGEFFYCNTSSLFNSSWIKRNYTETNSTNNETITLSCRIKQIVNMWQRVGRAMYAPPIPGEIRCDSNITGLLLTRDGGNKTNDGEVFRPIGGNIRDNWRSELYRYKVVAIEPLGVAPTRAKRRVVEREKRAVGMGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTTVPWNSSWSNKSQDEIWNNMTWMQWDKEVINYTSTIYELIEQSQNQQEKNEQDLLALDKWASLWXWFDITNWLWYIKIFIIIVGGLIGLRIVFAVLSIINRVRQGYSPLSLQTLIPNPRGPDRLGG"
  },
  {
    "reference": "MAMALAVGAPSEQRGNLPYNTLKDGPTVDSMKATTDGKNGQGESAFWDTCVHTVFREHCQRAPNSPAVNAWDGSFTYAELDSLSDAIASVLILSGVGPESIIPIYMQKSRWTTVAILGVLKSGGAFTLLDPSHPRSRVEEISKEIQARFILTSEKLSKQCLEMFSVLVVEHLSRACLPSPGQAGHTRSRPENAAYIAFTSGSTGKPKGIVIEHRSYCSGARSHLKVFGIDSTSRVLQFASYAFDVSIMETLSTLMAGGCLCVMSESERSDPNLFVVSYKNLRISHCFMTPSFARTVPWTECCNPPPTLIVGGELMRPSDARAYKEMGIRCMNAYGPAECSVNVSVQSRVEAAVDPRNIGYTTGATAWIISPENPEELMPTGTVGELLVEGPIVGRGYLNDPKATRQAFIDTPAWLRRHRKGTSYQHRVYRTGDLASLDSITGALLLHGRKDAQVKIRGQRVELPDIEHHLQLTLPNDNAEVIVEKVTFSDDGSEKLIAFVLVRPSNTDSVIGNTGDRLFLAPQSQIMEQFAISKKHLQTHLPSYMVPDIFIPISTLPQTASGKTDRKALRTRAAALSRRDVQCFLLSPAGGKRPPSTPKEATIRSLYSNVLNLPIDLIGMDDTFLRLGGDSLQAIRLVAAARAAGLILQAKDILSSQSTLAEQSKRAGLIQTIDRTWESSPPFALLHGPTRHAIVDLAQKQCRVPSNLIEDIYPCTALQEGMFITSLKHPGMYTGQIIFDIPDRMELPRLRAAWLSVVSENAALRTRI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that FtmPT2/FtmH catalyzes the prenylation of 12,13- dihydroxyfumitre-morgin C in the presence of dimethylallyl diphosphate, resulting in the formation of fumitremorgin B; The biosynthesis of fumitremorgin-type alkaloids begins by condensation of the two amino acids L-tryptophan and L- proline to brevianamide F, catalyzed by the non-ribosomal peptide synthetase ftmA; Brevianamide F is then prenylated by the prenyltransferase ftmPT1/ftmB in the presence of dimethylallyl diphosphate, resulting in the formation of tryprostatin B2. The protein have enhanced 2 ATP + L-proline + L-tryptophan = 2 AMP + brevianamide F + 2   diphosphate + 2 H(+).3. The designed protein should have ligase activity, phosphopantetheine binding within cytoplasm to regulate amino acid activation for nonribosomal peptide biosynthetic process, verruculogen biosynthetic process, nonribosomal peptide biosynthetic process, secondary metabolite biosynthetic process, brevianamide F biosynthetic process.4. We require a protein that can specifically bind to the metabolites produced in the Mycotoxin biosynthesis pathway. The designed protein sequence is ",
    "response#1": "REHCQRSPDSPAVNAWDGSFTYAELDSLSDAIASVLILFGVKPESIIPIYMHKSRWTTVAILGVLKSGGAFTLLDPSHPRSRLEEICKEIQARFILTSEELSKQCSEMSSVLVVEHLSRACLLSPGQAGQTPSRPENAAYIAFTSGSTGKPKGIVIEHRSYCSGARSHLKVFGIDSTSRVLQFASYAFDVSIMETLSTLMAGGCLCVMSESERSDPNLFVESYKNFRISHCFMTPSFARTVQWTECCNPPPTLIVGGELMRPSDTRAYKAMGICCMNAYGPAECSVNVSVQSRVEDGVDPRNIGYTTGATAWIISPENPEQLMPPGTVGELLVEGPIVGRGYLNNPERTAEAFFPAFGGQGPSNLNCFNDLLELNKTYGSTLRPLIHTADATLSELASLPHRSGFHEDEGFETLDWLQDPKQAPSREYLALSPMSFPINTLLSLCNYCVTLRALRLDPGQFRSSLHNVVGHSQGIFAAAAIAKADSWESFLEAAEIALKISFWVGLESHTAAPPSHISAAAVQDCVEHGEGQPSSMLGITGLNRAQVEMLVERVNKSLSDDDRHVCLALANSRDKYTIAGPPHSLRAVTYAELDSLSDAIASVLILSGVGPESIIPIYMQKSRWTTVAILGVLKSGGAFTLLDPSHPRSRVEEISKEIQARFILTSEKLSKQCLEMFSVLVVEHLSRACLPSPGQAGHTRSRPENAAYIAFTSGSTGKPKGIVIEHRSYCSGARSHLKVFGIDSTSRVLQFASYAFDVSIMETLSTLMAGGCLCVMSESERSDPNLFVVSYKNLRISHCFMTPSFARTVPWTECCNPPPTLIVGGELMRPSDARAYKEMGIRCMNAYGPAECSVNVSVQSRVEAAVDPRNIGYTTGATAWIISPENPEELMPTGTVGELLVEGPIVGRGYLNDPKATRQAFIDTPAWLRRHRKGTSYQHRVYRTGDLASLDSITGALLLHGRKDAQVKIRGQRVELPDIEHHL",
    "response#2": "REHCQRSPDSPAVNAWDGSFTYAELDSLSDAIASVLILFGVKPESIIPIYMHKSRWTTVAILGVLKSGGAFTLLDPSHPRSRLEEICKEIQARFILTSEELSKQCSEMSSVLVVEHLSRACLLSPGQAGQTPSRPENAAYIAFTSGSTGKPKGIVIEHRSYCSGARSHLKVFGIDSTSRVLQFASYAFDVSIMETLSTLMAGGCLCVMSESERSDPNLFVESYKNFRISHCFMTPSFARTVQWTECCNPPPTLIVGGELMRPSDTRAYKAMGICCMNAYGPAECSVNVSVQSRVEDGVDPRNIGYTTGATAWIISPENPEQLMPPGTVGELLVEGPIVGRGYLLDGADLSNGSPFVVDVGGHHGIDLMRVAEKHPDLPAGSLVLEDLPDVVGAVHLTTDKIRTVAHDLFEEGVEQPIKGARAYFMHAVLHDWSDETSVKILRQIAAVMKPGYSKVLINDIVIPSTGASCYQAAMDCLVLQASANERTEAVWSKVIKDAGRYTLTQGDPIGLQWTGGRAGGHHSHEDLHLPILKTYARIRRCSNIVLIAGSGFGGGSDTYPYISGDWSKSLSYPPMPFDGILLGSRVMVAKEAHTSPQAKQLIVQTEGVGDNDWHTSFETPTGGVITITSEHGQPIHMLATRGVMLWKEFDKRIFSIKDAAKRLSYIRAHREEIITRLNKDYQKPWFLTGTTGEIGSYLLDVLCNNDKVAKVWCLNRSADAFQRQVDSAKSKGLSSSWKSKAKFVRYDVASENLGLSQDGLEEIKNEATAIIHNAWEVNFNLPLS",
    "response#3": "AQCSRRPQASAIISWDGSWTYRELWAHSSFFARQLQRYGVTRGTPVAVCLDRSRWSIAVILGVLLARGTCVLIDLLAPRQRVRDILQIAGTGILVHSHATATLTSGLCPTVINVSFLAAQSDSSQPEFPFTLETWGGTPEDLAFIIFTSGSTGHPKGIEMPHRTLSTSISHHSAGMRVTSSSRVLHFSSYAFDVSIYEIFTTLAAGGTICVPSEFDRMNNLAGFIQDTQVNWAFLTPSTARSLNPADVPLLTTLVLGGEAVTHESVEVWAKGRSLINGYGPAEATICGVGNIPEAGWKSGVVGRIIGGLGWVTVPSDPNRLAAVGAVGELLLEGPFLARGYLGRPDLTAERFVADPYGRPGTRMYRTGDLVRWTRDGVLEYAGRADDQVKVRGHRIEPGEVEAALTALPQVSQSAVIAREDTPGDKRLVAYVVPAGHTDTAGTPGTAGTPGTAGTAGTAGTAGTAGTAGTAGTAGAAGTGEAALDAVRAAAAEQLPSYMVPAAVVVLEALPLTVNGKLDRAALPAPVFTAATTGYLNPAAGPGQGYAVVTFDGPGQGIMLRKYGLEMRPDWEAVTGRVIDFLEEYAAENPHLELDLNCIAVSGASMGGYYALRAAADQRVKACVSIDPFYDMWDFGTAHVSPIFIHAWTSGWISGGFDDSVLIRVVCVAINPVDGKSAEMSPTPGATSGTDFAGIVVALHGDAKSRTETADTIKTGDRVMGFVFGNNPHVLGNGAFAEYVTLPRRFLWRVP"
  },
  {
    "reference": "MSKYIIPAPVHGITADSRQVEKHGLFLAYPGQHGDGRDYIPDAIAKGANTIIWDSLGFDWNPEWTVHNIAIPDLKSQVGHIASQFYKDPTEQLWCIGVTGTNGKTTVTHWLAQAYRFLQRKSAVIGTLGNGTLDDLQPTQNTTPGPVELQKILANFLLEDVQMVAMEVSSHGLDQGRVNGVSFDVAVFTNVTRDHLDYHVTMEAYQSAKQKLFDWQTLSAAVINADDAFGVELIDRLRKQGKQVLSYGMQEADICATAVTMHATGFEIQVRTPQGNGLVQLHALGQFNVYNVLAVLGCLLANDVALPAALQAVSGLVPVAGRMQMFGGGDLPLVVVDYAHTPDALEKALQTLRIQARGKLSCVFGCGGDRDAGKRTEMGRIAGALADSVVVTNDNPRSENPYAIIAAISEGLTREFSMETDRAKAIAMAVMRANKGDVVLVAGKGHEDYQEIQGIRYPFSDAEWVLSALKKRAQS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Ideal protein should have a binding affinity for key enzymes in the peptidoglycan biosynthesis, Cell wall biogenesis pathway to enhance the overall efficiency of the pathway.2. The designed protein should have ATP binding, magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity within cytoplasm to regulate cell wall organization, peptidoglycan biosynthetic process, cell cycle.3. The designed protein have Mur ligase central domains with specific sequence motifs that are crucial for its activity.4. The ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate binding site should be located in a region of the protein that is accessible to the ligand.5. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.6. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MKLRQLLQGIECRQVAGELDSDISGISADSRKVTPGMLFVAVAGTKADGAAYAADAVARGAVAVVAGHAVEANVPVFTVSNPRRFLALASARFYGEPSERLTLVGVTGSNGKTTTKEMVASILRAWVGNDDALATSGNYNNDIGVPLTLLRLRANHKAAVFELGMNHPGEIAHLAELAQADVALVNNAQREHQEFMHTVEAVARENGAVIQALPADGVAVFPGDDAYSALWSEFAGQRRSLQFGLADTFEVYADHIHAEPAGTRFTLHTPGGTATVQLSAPGRHNLSNALAAAACAAVFGVPWARIQQGLQELAGVPGRMQLVAQRENGATVFVDYAHKPGAVIAALGSLRPHVMGRIVCVIGAGGDRDRGKRSLMGQAARDFADVVIVTDDNPRTEDPAAIRAAVMEGAGPEATEIPDRAEAILRGVDALLPGDALLICGKGHETYQEIGHEKHPFDDREEARAALEARGLL",
    "response#2": "MSLLCLDSRQVGAGDIFFACPGRASDGRQFVSQAVQAGAAAIVVADVSSDAYAGYKVPVITVPDVQDMLGQIGHEIHTRFSLVRVHKLELDSRLVSSGSTFVAVKGHVVDGRDFIDSAICQGANAVIAETEEPDSHGKIEWRGNIPIIYIANLTQHLSHIAVTGTNGKTSTVQWLAQALNAEGVACGTIGTLGVVLPDGTNLGATLTTPDVLSVHRCLAALRDAGARVVAIEASSIGLEQGRLQSVCIEIAAFTNLSHDHLDYHGTVQAYAAAKQQLFAWPGLRTAVINADDAVGRQFAQSCRAPQIETYSIEGDVAASLRATDIQTGSHGVIFNLALAHGVAQIMTRLVGRHNISNLLLVAGTLLALGYPFQVVSETASRLKPVCGRVEMVKVSDDFILLIDYAHNAMALSSILTTLREYHPGRLVCVFGCGGNRARSRRYEMGEVSGRMADFTIITSDNPRYENPEAIMDDIEVGMKKTTGKYIRIADRKEAIAYAIHHGKPGDIIVLAGKGHETYQIIGDRVLPFDDREVARAALAQLDHAQ",
    "response#3": "MKLRDLIARVDYRLVRGDVDIAIEADSRKVTNGSLFVCIKGFTVDSHNFAKQAAAQGAAAIIAERPVDVDVPVVLVTEIVNDSRKAREGVVFVCIKGAVADGHKYIPDVLKKNVSAIIVEDEVDLKAYGTDADGVTFIKTDDTRIAMARMAAVYYDHPSRKLRLIGITGTNGKTTTSYLLEGILTAAGHRCGVIGTIANYIGDRALPARRTTPESVELQRLFAEMVDAGCDTVVMEVSSHALALHRVHDCLFDVGVFTNLTQDHLDFHGTMEHYYNAKRKLFEMSEADRVGVINADDPYGDRLIRETPARVIRYGIDQPADVRAENVEMTVDGVSFTLVTPYGALEVNTRLVGRFNVYNALAAIAAGVAEGVPIEAIRRGLASVRVPGRFELVPATGLVGIVDYAHTPDALLNVLETIVHLREGNEQIITVVGCGGNRDAEKRPRMAAIACRHSDRVVLTSDNPRHEDPQLILDQMLQGVPPLDFKKVTVLPDRREAIARALELAEPHDIILIAGKGHEDYQIIGHEKIHFDDREVVREVLGIS"
  },
  {
    "reference": "MRQQTLKSSFKVTGVGLHTGKEYTLKLLPAPANTGYRIRRSDLDGKPEIPVFAEMVDFTDRCTKISCAQFSVATIEHALSALYGMGVDNCILEVDGPEFPILTGSAIPYVEGIKSVGIETQQDDRDIYIVKKTFEVEDPDTGARLILFPREETIIEANLQYDSNPPGRQHFTFCEKDSYEEAIASARSYVFVSEVIPLLHRGLIKGGSISNALIIKDRELTTEEETLLRKEIPEYKTGSTLGYINPSENLGENEPARHKILDLLGDLALAGRYFKGHLIATKPGHTINNKLARLLRNEIKQSETQEPYYNPDDDPILNIEQIRELLPHRYPFLLVDKVIEMGHNSIVGLKAVTGNEPFFEGHFPDEPVMPGVLIVEALAQTGGLLVINALKDEGTWSTYLLKIDNVKFRRKVVPGDTLLFCLKLLTEIRRGIARMRGLAFVGSRVVCEAEFTAQIVKNNPREEK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Involved in unsaturated fatty acids biosynthesis2. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.3. The designed protein must have a Proton donor, active site that is structurally stable and resistant to denaturation.4. The ideal protein would be one that plays a critical role in fatty acid biosynthetic process, lipid A biosynthetic process through 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity, metal ion binding, UDP-3-O-acyl-N-acetylglucosamine deacetylase activity, UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity within cytoplasm.5. The protein should be optimized for activity in Glycolipid biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MSSGAMTAADAQNPVEEAVNALKAGKMVILVDDEDRENEGDLVMAAQFATPEAINFMAKHGRGLICLAMTEERCEELELPLMVSHNTSSFGTAFTVSIEARTGVTTGISAADRAHTIEVAVRPQTKPEDLVRPGHIFPLRAKKGGVLVRAGHTEAAVDLARLAGLYPAGVICEIMNDDGTMARVPELMEFARKHGLKICSIADLISYRLRHEKLVQREVRLQKSEPSVFGV",
    "response#2": "MKNDKNINQHVTTPGRNMAGARALWRATGMKDGDFHVIGVSNSLKKQFELAGLYDVKVPAISQITDEMAEYDKQTWDIMTSDEGNYTQTFSDAMWSDITSSDEGIRTMFTKDQTRAVHTAGDEGMRTLESSIKGGVTTVQLFSSADTQTQLEDLKPEFDYNKVYTTWKEGDQARDEAGGVIATMAKEMTEGRK",
    "response#3": "MNPITPDLKAAIADAXRXADXAXVXGXRXXSXDXAXAXDXDXXAXT"
  },
  {
    "reference": "MRLDKFLAENTGLTRSQANKVLKQSAVTVNGHVEKNGAQKVSQTDEICLEGEHLPWVSAGQYLMLYKPQGYVCSHEDGDYPTIYQFFDYPLAGKLHSAGRLDVDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADPVESHYQQACAEGMLLRGEKTPTKPATLEILDDYNVNLTISEGRYHQVKRMFAALGNKVVGLHRWKIGQIELDDRLAEGEYRSLSAEEIATFNK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Responsible for synthesis of pseudouridine from uracil-516 in 16S ribosomal RNA..2. The designed protein have S4 RNA-binding domains with specific sequence motifs that are crucial for its activity.3. The designed protein must be able to perform rRNA pseudouridine synthase activity under specific conditions. The designed protein sequence is ",
    "response#1": "IIVINKPRDFVVHPGAGQADKTVLNALLFHYSPIAEVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVRALQKRDVTREYEAIVIGTMTAGGMIDKPIGRHSTKRTLMSVSPMGKHAVTHYRVAEHFREHTRLRLRLETGRTHQIRVHMAYLQHPLLGDTAYGGRARIPKGATEELTEMIRDFDRQALHAVMLKR",
    "response#2": "YFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLDIDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDLTKPAVLEVITPTQVRLTISEGRYHQVKRMFAR",
    "response#3": "ILVLNKPPKVPMKGNLPVHNSMDVLAAAALSYGNEEGPKLVHRLDRESSGLLLFGRTKESFTRLHWLFTSVNLAKTNSQVWNAACEAYMQRYWALVIGTPKEREGIISAPLSKVLLDDGKAERVILAHPSGIDGAQEAVTAYRVMGPTIHGCSWIELRPLTGRKHQLRVHCAEALGTPIVGDYKYGWFVHQRWKQNPL"
  },
  {
    "reference": "MKPNLEKRSLYSFTVEELRDYFKENGLSKFVADQIYDWVYKKSVFEPENWSNISNKVKTFLNENLLLTLPKIVWDGHSVDGTRKFLVAMSDGKTVETVAIPAKNDRLTLCVSSQVGCAIGCTFCHTGTMGLTRHLDSSEIVGQFLTVTRFLKEKVGESERLTNIVYMGQGEPLHNFEHVKKATQIFMEDKGLMLGQRKITLSTSGLVPQIEKLNDFPPVNVAISLHAAHNNIRSELMPINKAFDLERLLKAIRTINLKAHRWITYEYILIANYNDRPEDIEALGELLDKKDSKINLIPFNEYPDSNFKRPSDERIKWFQDQLMKRGLITTVRTTKGADILAACGQLKSEMEDKLNLWD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs2. The designed protein should contain one or more Radical SAM core domains that are essential for its function.3. The presence of [4Fe-4S] cluster is necessary for the protein to function in the desired environment.4. The designed protein should have a binding site that is specific to the target S-adenosyl-L-methionine, [4Fe-4S] cluster ligand.5. The designed protein is the optimization of the S-methylcysteine intermediate to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.6. A protein that localizes to cytoplasm and can perform tRNA binding, 4 iron, 4 sulfur cluster binding, metal ion binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "LAGMTRNQIRDCLIATGLVEEKKAKMRSGQIWRWVYQYGITDFAAMTDISKELRERLPEHVELLHPTVVAQHSSRDGTIKWLLRLRDGNEVETVYIPKPSFRAKQIYNWLYKQYASSYDEMKNIPKDLKEELEEKVLVGLDRSALADRLAGLGVPERQRRMRVSQLWSWIYVRGQTDFAAMTDIAKELRAELASHPRVGRSGALCVSSQVGCTLNCTFCHTGSQALVRNLTSAEIVGQILVARDDLDEWPSPSEGRKITNVVFMGMGEPLYNLENVLTATGIISDGEGISISRRRITVSTSGIVPKMHELGEQGQAMLAISLHAVHDDLRNELVPINKKYNLAKLLEACRTYPGASNARRITFEYVMLKGVNDSDAEARKLVRLLAGIPAKINLIPFNPWPGSVYECSSWGRIEAFAEIVNKAGYASPIRGNRLRACGQLAAS",
    "response#2": "QNLLDLDADALIAWFAERGEKPFRARQILRWIHRFGETDFDAMTDVAKSLRALLKETACIQPPVPIRDNLSTDGTRKWLMDVGNGNAVETVFIPEATRGTLCVSSQAGCALDCAFCSTGKQGFNRNLSAAEIIGQLWLANRLLGAEPNGERIISNVVMMGMGEPLANFDSVVSALKLMLDDHAYGLSRRRVTVSTSGIVPAMDRLRDECPVALAVSLHAPNDALRDKLVPINQKYPLKDLLAACNRYLDKAPRDFITFEYIMLEGVNDGDEHIRQLIDIAREVPCKYNLIPFNPFPDSGFHRSSGPVVRRFSQLLNEAGIVTTTRKTRGDDVDAACGQLAGQVKDKTRRTQRLLDAHQA",
    "response#3": "ETDRGTLCVSSQAGCAVGCRFCSTGHQGFSRNLSTAEIIAQLWFAEHFLRKKLGTSERVISNVVMMGMGEPLQNYAALIPALRTMLDDHGYGLSRRRVTVSTSGVVPMMERLGVDCPVALAVSLHAPNDALRDHLVPLNKKYPLSELMHACHQYLTHAPRDFVTFEYCMLDGVNDTQEHAQQLIHLVQQDGRGTSWCKFNLIPFNPFPASGLRRSPHAAVQAFAARLNAASIVTTVRKTRGDDIDAACGQLAGDVGRSGALCVSSQVGCTLNCTFCHTGTQALVRNLTAAEIVQQVLIAKDDLAEWPSRLEDRQITNVVFMGMGEPLYNLDHVADAIDVISDGEGIGIGRRRITVSTSGVVPKIEELGARTNTMLAISLHATNDALRDELVPLNKKYPIAELLDAIRAYPELSNSKRVTFEYVMLKGVNDSPGEAKALVRLLQGIPSKINLIPFNPWPGSPYECSDWATIERFADIVNKAGYASPIRENKEDLKMA"
  },
  {
    "reference": "MTITPQQLIALLPLLIVGLTVVVVMLSIAWRRDHFINTTLTVIGLNLALLSLYFVGQQEAMDVTPLVHVDRYAMLYIGLVLVASLATATFAYSWLENYPDNKEEFYLLVLIATVGGILLACANHLASLFIGIELLTLPLFGLIGYAYRQKRSLEAAIKYMLLSAAASSFMLFGIALLYAESGDLSFAALGHRLSDSYIYQPLILSGLGMLVVGLGFKLSLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFAVVIRLFVEAPMADSEALRTVLTVIAIASMLFGNLMALTQTNIKRLLGYSSIAHLGYLLVALVAVQNHQLSEETVGIYLAGYLFSSIGAFGVVSLMSSPYKGPDADSLYSYRGLFWHKPILSAVMTVMMLSLAGIPMTFGFIGKFYVIVLGVKAELWWLTGAVVVGSAIGLYYYLRVMVSLYLPAPKALNRDTPRNWASTAGGMVVLISALLVLALGIWPQPLIDVIQQAKIIL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.2. The protein can be targeted to oxidoreductase complex and perform NADH dehydrogenase (ubiquinone) activity.3. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "QDMLLFFLMWELELIPVYLLLSIWGGANRLYAATKFILYTALSSLFILVAGLAMAFYGDPISFDMTDLAHKTYPLTFQLLMYGAFLIAYGVKLPIFPLHTWLPDAHGEATAPVHMLLAGILLKMGGYALMRMNAGMLPDAHLYFAPVLIVLGVVNIIFAALTSFAQRNLKRKIACSSISHMGFVLIGIGSLTEIGMSGAMLQMISHGLIGASLFFLVGATYDRTHTLELEEMGGVGLKMPKIFSMFTACSLASLALPGMSGFVAEIMVFIGMATTDAYSF",
    "response#2": "QDMLLFFLMWELELIPVYLLLSIWGGANRLYAATKFILYTALSSLFILVAGLAMAFYGDPISFDMTDLAHKTYPLTFQLLMYGAFLIAYGVKLPIFPLHTWLPDAHGEATAPVHMLLAGILLKMGGYALMRMNAGMLPDAHLYFAPVLIVLGVVNIIFAALTSFAQRNLKRKIACSSISHMGFVLIGIGSLTEIGMSGAMLQMISHGLIGASLFFLVGATYDRTHTLELEEMGGVGLKMPKIFSMFTACSLASLALPGMSGFVAEIMVFIGMATTDAYSN",
    "response#3": "QDMLLFFLMWELELIPVYLLLSIWGGANRLYAATKFILYTALSSLFILVAGLAMAFYGDPISFDMTDLAHKTYPLTFQLLMYGAFLIAYGVKLPIFPLHTWLPDAHGEATAPVHMLLAGILLKMGGYALMRMNAGMLPDAHLYFAPVLIVLGVVNIIFAALTSFAQRNLKRKIACSSISHMGFVLIGIGSLTEIGMSGAMLQMISHGLIGASLFFLVGATYDRTHTLELEEMGGVGLKMPKIFSMFTACSLASLALPGMSGFVAEIMVFIGMATTDAYSW"
  },
  {
    "reference": "MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKTLGQMSPEERKTIGAHINECKNRFQTAFNAKREALNEVKLQARLAAEALDITLPGRAQEGGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYVENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEGVTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGMVHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFAK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+) binding.2. A protein that localizes to cytoplasm and can perform magnesium ion binding, ATP binding, phenylalanine-tRNA ligase activity with high specificity and efficiency.3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "FFKQNGFAIEFGPEIESDYYNFETLNIPSHHPARAMHDTFYIDETHVLRTHTSGVQIRTMEKQQPPIRIIAPGRVYRCDSDITHTPMFHQVEGLLVDKDVSFADLKGLLHAFLNSFFEKDLKVRFRPSYFPFTEPSAEADIECVMCDGKGCRVCKHTGWLEVLGCGMVHPKVLKAGNIDSEKYQGFAFGMGVERLSMLRYGIDDLRMFFK",
    "response#2": "MPKVNINRDILYKALGFYIKTYTQEQFEDLCFAFGVELDEVTSEREMKKNETGVEDLTVSDDVIYKIDVSANRYDLLCLEGIARALNVYNHN",
    "response#3": "MPKVNINRDILYKALGFYIKTYTQEQFEDLCFAFGVELDEVTSEREMKKNETGVEDLTVSDDVIYKIDVSANRYDLLCLEGIARALNVYNHY"
  },
  {
    "reference": "MEKSLIILKPDAVQRGLIGPILTRLEARGLKFIGIKLSQVSSELAHKHYGVHEGKPFFAGLVSYITSGPVLVVAVEGKDVIEIVRSTVGATNPVKAAPGTIRGDFGVNIGRNLIHASDSPENGDHEVALFFSSEELIRSERSVDQWITE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Major role in the synthesis of nucleoside triphosphates other than ATP.2. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.3. A protein with metal ion binding, nucleoside diphosphate kinase activity is needed to enhance nucleoside diphosphate phosphorylation in a specific context. The designed protein sequence is ",
    "response#1": "MERCLILVKPDGVERNLIGEVISRFERKGLAIKALKMMRVTREQAEDHYSVHRLKPFFDDLVSYLTSGPIVAMIVEGNNAIASSRMLAGATDGSKAAPGTIRGDFSLDIERNIVHASDSLESYEHEYKIFFNENEIMY",
    "response#2": "MERCLILVKPDGVERNLIGEVISRFERKGLAIKALKMMRVTREQAEDHYSVHRLKPFFDDLVSYLTSGPIVAMIVEGNNAIASSRMLAGATDGSKAAPGTIRGDFSLDIERNIVHASDSLESYEHEYKIFFNENEIMY",
    "response#3": "MERCLILVKPDGVERNLIGEVISRFERKGLAIKALKMMRVTREQAEDHYSVHRLKPFFDDLVSYLTSGPIVAMIVEGNNAIASSRMLAGATDGSKAAPGTIRGDFSLDIERNIVHASDSLESYEHEYKIFFNENEIMY"
  },
  {
    "reference": "MEEAASPARRRGGPAGVSAVQTDTIEALTELEDLERVYAQLCAEEAEVQVELDALVGQQNNIETKMLSLQRMGPNLQLIEGDAVQLSGMINFTCSLAENVSSKVRQLDLTKKRLYQAIQRADDILDLKFCTDGVQTALRNQDYEQAAAHIHRYLSLDQSVIELSKQGGEGSAVEASLALLQEAERNLKTLVTTRLEEAVATGDLPQVERFFKILPLLGLHEQGLARFAQYLCSQLASKAEENLILAVGSDLGERRAPVIFADTLTLLLEGIARIVETHQPIVETYYGPGRLHTLLAHLQKECDKQAQKIVDKFIQQRDYNNKFQVVQSNMMRGMTTDKIEPRDLDPVLCEVTLMNSRAELYFRFLRRRIVADFEVADAMADEAVIQEHQQSLEQLLKNCQLSRTMQELIGYYIPMEEYYMRESVNKAVAMDTAEVGQLSSSVVDDVFYIVKKCISRALTSSSSDCVCAMINHATSVLETDFREVLVCKLRAGYPVSALQDLQRGVSSAVSLMQSSLQQGKITNLTQTLGIESQEQAKSAYLVTLNNVEVCSENISTLKKNLESDCAKLFSQGASSDHAKEKIDSCLSDLVNTSSKFKDLLQEGLQELNNTAIRPQVKPWISSFLSVSHNIEEEEFSEYEANDPFVQQLIVQLEQLMAEFKVGLSPVIYDTLTSLMTSLIAMEMEKTVFKCTFSRLGGLQFDKELRALVAYLSSVTSWTIRDKFARLTQMATILNLERVSEILDYWGPNSGPLTWRLTPAEVRQVLALR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Plays a role in the vesicular trafficking between the endoplasmic reticulum and the Golgi apparatus .; Required for normal Golgi function2. The protein should be engineered to have a higher affinity for a specific molecule or receptor in Golgi membrane that is critical for skeletal system development, retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum, Golgi vesicle prefusion complex stabilization, proteoglycan metabolic process. The designed protein sequence is ",
    "response#1": "EPGARSMNISIWGFTESFLTRQWVSYTIDFKDILERNCEENDYTTWLAHSTDPGDYKDGCILGYKEQFLRLRKSSVCQNGRDYVVAKQPSICPCSLEDFLCDFGYFRPENASECVEQPELKGHELEFCLYGKEEHLTTNGYRKIPGDRCQGGMNPAREVKDLKKKC",
    "response#2": "EPGARSMNISIWGFTESFLTRQWVSYTIDFKDILERNCEENDYTTWLAHSTDPGDYKDGCILGYKEQFLRLRKSSVCQNGRDYVVAKQPSICPCSLEDFLCDFGYFRPENASECVEQPELKGHELEFCLYGKEEHLTTNGYRKIPGDRCQGGMNPAREVKDLKKKC",
    "response#3": "KAGEVLIKQTNITLDTINKKISNYLIDYMYTFESNSNMGTKHVIDPTERNLALFQKGLVYLSNDIQYYDDFLKRVTTMRSKTLLDEFLSQFGTTSELSTTISSSEDPVRYIGDVLATIHTMIANEVDFVKSLFKFTSEDMDKSSSMIINNNAQYFDGLDLKLVNDIVQYLANSCKLRIEQVIRFEENKVINFEITQLLDLYSSMFVNKGIRDDNPLVLHLIQLRDISEKKIINSLTKQLAETENTQISSPDLLPPQWLSDYLNSITELFDHVERLYGGKRFSKDDIDSDKYMFPYDTLKTVIEEPFMTSLIKQIKDSYPLAKKKEEIRIVMLTLQINCFEMINFRLQPYSLSIFSYDESCKHILENIQHTLDETVEKLQKLQISLLFERTGLNMYYNLMNMIFPIDSIQDEIDYDMYMSLVDNPLMSLENLEKNIHEKLNDYLPQALPS"
  },
  {
    "reference": "MAFASVLKDAEITAALDGCKAAGSFDHKKFFKACGLSGKSADEVKKAFAIIDQDKSGYIEEEELKLFLQNFKAGARALSDAETKAFLKAGDSDGDGKIGVDEFAAMIKG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must be able to perform calcium ion binding under specific conditions.2. For general function, the protein need meet that It binds two calcium ions.; In muscle, parvalbumin is thought to be involved in relaxation after contraction3. The designed protein have EF-hand domains with specific sequence motifs that are crucial for its activity.4. The designed protein should have a binding site that is specific to the target Ca(2+) ligand. The designed protein sequence is ",
    "response#1": "MGKEIVKEAFRLFDENQDGFIDENELKHVLSLLGYDKDGNGFISAAE",
    "response#2": "EEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVAMMG",
    "response#3": "MF"
  },
  {
    "reference": "MRITLPLLSTAVGLGLTAAVLGTGPAATAAAPQEPVRAAQLGYQPSAGSGEDAAANRAFFEAVVKSVAEKRAANPSAAAAVTVYYSATNAPSFRSQISRSAQIWNSSVSNVRLAESSSGADFAYYEGNDSRGSYASTDGHGSGYIFLDYRQNQQYDSTRVTAHETGHVLGLPDHYSGPCSELMSGGGPGPSCTNPYPNSTERSRVNQLWAYGFQAALDKALEKASQR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The Zn(2+) binding site should be stable and able to withstand changes in environmental conditions.2. The designed protein localizes to the extracellular region.3. The designed protein must have a active site active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "PEELPEQEPWTAVQLARRLERPTTLDYLRSVFDDFEELHGDRASGDCPAIVGGTALLEGRPVVVIGHQKGHSVNELSTRNFGMPTPAGYRKAARLMRLAGKLGIPLVTFIDTPGAFPGAEAEEHGQSIAIAECIRTMAGIPVPAVAIVTGEGGSGGAIALGVANTVLALSNAIYSVISPEGCAAILWNDPAAARRAAAALRVDTPNLLRFGVVDGVVQEPEPGADRDHPGTADRVRAALSATLQAS",
    "response#2": "PEELPEQEPWTAVQLARRLERPTTLDYLRSVFDDFEELHGDRASGDCPAIVGGTALLEGRPVVVIGHQKGHSVNELSTRNFGMPTPAGYRKAARLMRLAGKLGIPLVTFIDTPGAFPGAEAEEHGQSIAIAECIRTMAGIPVPAVAIVTGEGGSGGAIALGVANTVLALSNAIYSVISPEGCAAILWNDPAAARRAAAALRVDTPNLLRFGVVDGVVQEPEPGADRDHPGTADRVRAALSATLQS",
    "response#3": "PEELPEQEPWTAVQLARRLERPTTLDYLRSVFDDFEELHGDRASGDCPAIVGGTALLEGRPVVVIGHQKGHSVNELSTRNFGMPTPAGYRKAARLMRLAGKLGIPLVTFIDTPGAFPGAEAEEHGQSIAIAECIRTMAGIPVPAVAIVTGEGGSGGAIALGVANTVLALSNAIYSVISPEGCAAILWNDPAAARRAAAALRVDTPNLLRFGVVDGVVQEPEPGADRDHPGTADRVRAALSATLQAA"
  },
  {
    "reference": "MAQACDLIAEDIQHYLQVQQTKGMLRFITCGSVDDGKSTLIGRLLYESKMLFEDQLGQLEADSKRQGTQGGDLDFALLVDGLSAEREQGITIDVAYRYFATDKRKFIVADTPGHEQYTRNMVTGASSADVAVILVDARKGLLTQTRRHSYLVSLLGIRQVVLAINKLDMVGYSQAVFQRIEAEYRAFAAQIGLHTIQCIPLSALRGDNLIEASTQTPWYQGPTLLEHLENVPLQDEAQGAQPFRLPVQWVNRPNLNFRGYAGSIVAGSIRRGERIRVLPSGKESRVASILTPAGERDIAICGQAVTLCLEDEIDISRGDLLALTDAPPASADQFEATLVWMDEEALLPGRPYLLKIGTRTLGVTCTELKHKVNVNNLEHLAARTLELNEIGLCNLHLDQAIAFDPYTENRDTGGFILIDRLSNNTIAAGMLHFALRRAQNVHWQAIDVNRQAHAELKRQQPLILWFTGLSGAGKSTIANLVEKKLHALGRHTYLLDGDNLRHGLNKDLGFTEADRVENIRRVAEVARLMLDAGLITLVSFISPFRAERAMARALVAEGEFVEIFVDTPLELAEQRDPKGLYQKARRGELKHFTGIDSPYEAPEHPEIRIDTRADSPQQAAEAIVAYLLAHIPGR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must be optimized for sulfate adenylyltransferase (ATP) activity, adenylylsulfate kinase activity, GTP binding, ATP binding.2. The design of the protein must focus on the precise arrangement of amino acids in the Phosphoserine intermediate to enable effective catalysis of adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate   + ADP + H(+).3. The ATP binding site should be stable and able to withstand changes in environmental conditions.4. Ideal protein should have a binding affinity for key enzymes in the sulfite from sulfate: step 2/3, Sulfur metabolism, hydrogen sulfide biosynthesis pathway to enhance the overall efficiency of the pathway.5. The protein must exhibit the following characteristics: APS synthesis involves the formation of a high-energy phosphoric- sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.. The designed protein sequence is ",
    "response#1": "RLLTCGSVDDGKSTLIGRLLYDAKMIYEDQLAAIEEDSVKSGTTGDDIDLALLVDGLQAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRNMATGASTCDLAIILIDARYGVQTQTRRHSFLASLLGIKHIIVAINKMDLVDYSQDTFLSIKKDYTEFTKKLAPADFQFIPLSALNGDNVVNPSKHMSWHTGLPLMSMLESIEIKSMLRFITCGSVDDGKSTMIGRLLYESKLVFEDQLSALETDSRKSGTQGANLDFALLVDGLAAEREQGITIDVAYRFFTTTKRKFIVADTPGHEQYTRNMVTGASTADLAVLLVDARKGVLQQTRRHSYLVSLLGIRHVVLAVNKLDLVDYSQEVFDAIDRDFRAFAGRIGAPSVVTVPMSALMGDNLTGPSPNTPWYQGPSLIEHLESVEIDDDRQARPFRMPVQWVNRPNLDFRGFSGLIASGVVKPGDRVRVLPSGQESTIDRIVTLPDDLPQAVAGQSVTLTLCDEIDISRGDLIAHPQSEVSDQFEATIVWMDEEALLPGRGYWMKLASQTVSATVAEPKYEININNVGGPGDHLAAKTLELNSIGVAEVTTDRPVVFEPYADNRALGGFILIDKITNRTVGAGMLNFSLRRSQNIHWQSLDVTRERRAALKQQKPCVLWFTGLSGSGKSTIANLVEKKLHALGRHTYLLDGDNVRHGLNRDLGFTDADRVENIRRVAEVARLMADAGLIVLTAFISPFRAEREMARDLMGENRFLEIYVDTPLDVAEERD",
    "response#2": "LTCGSVDDGKSTLIGRLLYDAKMIYEDQLAAIEEDSVKSGTTGDDIDLALLVDGLQAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRNMATGASTCDLAIILIDARYGVQTQTRRHSFLASLLGIKHIIVAINKMDLVDYSQDTFLSIKKDYTEFTKKLAPADFQFIPLSALNGDNVREIDANLVWMDPEAMKPGKVYLVKHTAQLLKGTVTSLAFRIDPETLNRQKAETLKLNEIGRVTIQLFRQIFCDEYTQNRETGSFIMIDPLTHATVAAGTQVADQFEATIVWMDEQPMLRGRSYLMKIGAKTVTATIAPVKYQLSVNTLEHLAAEKLELNEIGVCELELDRNIAFDPYTENRDTGGFILIDRLANRTVAAGGTIASGTVAPGDQVVVAASGKATRVARIVAAEGDLDRAEAGQAVTLTLADEVDVARGDVLIHLEQRFDAMVVWMHDEQMVPGKNYVFKMGAKNVTGTIGTLRYGVDVNTLHRQEAPTLKLNEIGRCNVSLSEPVCFDAYRRNRLTGSFIVVDRITNATLAAGMQVSDQFEASLVWLDTAPMLPGRRYLLQTASATVGATVTQIKHAVELETLGHACARMLECNQIATVTLRLDRAIPFVPYVECNTLGSFILIDRETNRTLAGGMLHFALRRAHNIHWQAADVTRESRKAMNGHKGKVIWFTGLSGSGKSTIANEVEKKLYALGRHTYILDGDNIRMGLNKDLGFTDADRVENIRRVAEVAKLMADAGLIVLVSFIS",
    "response#3": "KTMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALENDSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFSTEKRKFIVADCPGHEQYTRNMVTGASTADLAVILIDARKGVLVQTRRHSYLCHLLGIRNIVLAVNKMDLIDYDQARYDAIVAEYETFAKGIGIESFTAMPISGFKGDNITANSPNTRWYEGAPLVQHLEAVEINAAHDAADLLRFSTAGSVDDGKSTLIGRLLYESNGLYEDQLAAVRKDSEKFKREELDLSLVMDGLKAEREQGITIDVAYRYFSTPRRHFIIADTPGHEQYTRNMATGASTADLAVVLIDARNGVLVQSKRHAFIASLLGIQHMVVAVNKMDLVGYSEEVFRKIQADFGTFAEKLRISDLTYIPVSALVGDNVIRKSERMPWYDGMPLLSHLENVHVMGTRNFRMPVQYVNRHRSDFRGFAGTVSQGRIAPGDPIMVMPSGRQSRVERIVTFGGDLDAASAGQAVTLTLSDEIDISRGDLLVRPNNQVSDQFEASLVWLDTAPMLPGRRYLLQTASATVGATVTQIKHAVELETLGHACARMLECNQIATVTLRLDRAIPFVPYVECNTLGSFILIDRETNRTLAGGEVADQFEATIVWMDDEAMLPGRAYLLKLATQTVSATIQAPKYEINVNTMEHLAAKTLELNVIGVAEITTDKPLVFEAYAENRALGGFILIDKINNRTVGAGMLNFSLRRSQNIHWQHLDVDKAAHARLKGQKPKVLWFTGLSGSGKSTIANLVEKKL"
  },
  {
    "reference": "MTRPHEQEAGIDEPHTGERPPQGETSEPREEFRGPEAYKPIESLDPSLRLPLTFTERRRGKPPRHLADLTLAERKGVLTEAGLPGFRADQLSRHYFTHLTRDPEAMTDLPAADRAEMVERLLPPLATEVRRLTADGGETVKTLLELFDGTRIESVLMAYPGRNTVCISSEVGCGMACPFCATGQMGLTRNLSTAEIVEQVRIAAAACASGELTGEPARLSNIVFMGMGEPMANYNAVMGAIRRLTQPAPEGFGISARNITLSTVGLVPAIRRLIDEKVPVTLAVSLHAPDDELRDDLIPINSRWKVGELLDAARAYFEATGRRVSIEYALIKDINDQRWRAGLLADELNRRGHGWAHVNPIPLNPTPGSMWTASEPEVERIFVDTLRNAGITTTVRDTRGSDIDGACGQLAADVLRRK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should emphasize Basic and acidic residues compositional bias.2. The protein must be engineered to have a high affinity for [4Fe-4S] cluster in order to increase its catalytic activity.3. The protein should have rRNA binding, tRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding, tRNA binding, metal ion binding and be active in cytoplasm.4. The S-adenosyl-L-methionine, [4Fe-4S] cluster binding site should be located in a region of the protein that is accessible to the ligand.5. The S-methylcysteine intermediate, Proton acceptor of the protein must be engineered to enable effective catalysis of adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.6. The designed protein should contain one or more Radical SAM core domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MTACVSSQVGCSLSCKFCATGYMERKRNLNPDEIYDQVVLIAKQANDNYQMPLSNIVYMGMGEPLLNYSNVMKSIERITAEDGLNMAAKRITVSTAGIAKMIKKLGDDQVRFNLALSLHAANDEKRNTIMPINEQNSLKALAEALKYYFAKTKNPVTYEYIVFDNFNDGIEDAIELAKFCRHLPCKVNIIEYNPISFAAYQNAETDKIDAFADYLAGADGQDLVNPQRQNLLGMSREQMEAFFLAIGEKKFRASQMMKWIHHEGCDDFAAMTNLSKPLREKLARLAEVRGPGVVYEGASNDGTRKWVLEVEDGSYVETVLIPADNGKRRTLCVSSQVGCSLDCSFCSTGKQGFQRNLTAAEIIGQVWVAQRSVGPRRDSGNRPVTNVVMMGMGEPLLNYDNVVPAMKLMLDDNGYGLSKRRVTLSTSGVVPMIDKLGDDIDVSLAISLHAANDELRTELVPLNRKYNIRCLIDACHRYLSKCADNRLITIEYTLIKDVNDQQQHAEELATLLDELPCKINLIPFNPFPHSGYEKPSRNQCMRFQQWLYDLKHTATIRTTRGDDIDAACGQLVGQVKDRTKRHKRYRTAADQLLASRD",
    "response#2": "MTACVSSQVGCSLSCKFCATGYMERKRNLNPDEIYDQVVLIAKQANDNYQMPLSNIVYMGMGEPLLNYSNVMKSIERITAEDGLNMAAKRITVSTAGIAKMIKKLGDDQVRFNLALSLHAANDEKRNTIMPINEQNSLKALAEALKYYFAKTKNPVTYEYIVFDNFNDGIEDAIELAKFCRHLPCKVNIIEYNPISFAAYQNAETDKIDAFADYLEALAAKVEPAPSKPSLIGLDRVALADRLTDLGVPERQIRMRVAQLWNRLYVGGAQTFDDMTTLSKDLRHALDSQVSLDRPEIVSEQISTDGTRKWLLRLAPGRPGEAAPEIETVYIPEEDRGTLCVSSQVGCTLTCTFCHTGTQRLVRNLTPGEIVGQILIARDRIGEWPGATRPTDGPLPNAERLITNIVLMGMGEPLFNFDNVRQAMEIAADGDGISVSKRRITLSTSGIAPMIPRCGVELGVMLAISLHAVRDDLRDVLVPINRKYPIAELLDACRAYPSLSNAKRITFEYVMLRGVNDSLADAKALVRLLAGIPAKINLIPFNPWPGAPYESSEWEQIEKFAEIINKAGYASPVRTPRGRDIMAACGQLKSASEKMHARKQGQTLAF",
    "response#3": "MTACVSSQVGCSLSCKFCATGYMERKRNLNPDEIYDQVVLIAKQANDNYQMPLSNIVYMGMGEPLLNYSNVMKSIERITAEDGLNMAAKRITVSTAGIAKMIKKLGDDQVRFNLALSLHAANDEKRNTIMPINEQNSLKALAEALKYYFAKTKNPVTYEYIVFDNFNDGIEDAIELAKFCRHLPCKVNIIEYNPISFAAYQNAETDKIDAFADYLRDTIAPSTPWQSLLDISLEHLTQRLETWGEPQFRAQQIWQAIYHDLATSPDEITTLPKVLREKLKAEFTLGSLSPAAEARSSDGQTRKRLFRLPDGKSIEAVLMGYEKRRTTCISTQAGCAMGCVFCATGQMGFRRHLRAGEIVEQVLWFARELKANGDRLTNIVVMGMGEPFHNYEATMAAVDLLNDPAGFNFGARRITLSTVGLAPMIERFAAERRQVNLAVSLHAATDELRSELLPINKKYPLDVLFEAVRKYYALTKRRVTFEWALIQGKNDTAAQARALADLAVGLPCHVNVIPLNPTRDYAGQATTRERAAAFKAALESCGLPCTIRVRRGIDINAGCGQLAVEEQLRAQAPQHRS"
  },
  {
    "reference": "MNQQATKSLSQLGLRTRDGRDAVITGLSVDSRTVKPGHLFAALPGTTVHGAEFITYALRMDAGAVLTDRAGEALAADVLKDWPGALIIAEDPRAALAGAAALWFEAQPEHVVAVTGTSGKTSVASFTRQIWQALGHKAISLGTMGVEGDYHAKLAHTTPEPITLHRMLSEAGAAGVTHAAMEASSHGLDQRRLDGVRVEAGGFTNFSQDHLDYHAGFDEYFAAKALLFNHILEEGESAVINIDGERGRQMAEIARDRGLAVTTVGQGQDAMLRILGQRYDATGQDLRFSLMGQTQMVRLPLIGGFQAENVLMAAGLALASGDSAERILSVLPQLQTVRGRMQLVAQRENGAAVFVDYAHKPGAVIAALQSLRPHVMGRIVIVLGAGGDRDRGKRPLMGQAAREFADVVIVTDDNPRSEDPAAIRAAVMEGAGPEAIEVGDRAEAILRGVDALQPGDALLIAGKGHETGQLVGQDLYPFDDAEQASVAVAALDGKI",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein with ATP binding functions is required to enable regulation of cell shape, cell wall organization, peptidoglycan biosynthetic process, cell cycle.2. A protein that can modulate the overall flux of metabolites through the peptidoglycan biosynthesis pathway, enhancing or suppressing its activity as needed.3. The designed protein must have at least one Mur ligase C-terminal, Mur ligase N-terminal catalytic, Mur ligase central domain.4. The protein should have a high affinity for meso-2,6-diaminoheptanedioate.5. The protein should have a low Km value for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "MLKISGLTADSRKVRPGFLFAAFKGASVDGTRFAPDAAAAGAVAVLCADDTMDSVRAACPQHMIVIGAPEPRQVFARMAARFYGEPSKSLRLIGVTGTAGKTSVAHFVRQIFQAAGHPAASLGTLGTIRPGGESYGGLTTPDPVALHKELAELEAEGIGHAALEASSHGLDQFRLDGVRLTAAAFTNLGRDHMDYHATVEDYLKAKLRLFSDLLPAGGTVVVDPGEKYADRVLAVAEARGLPLLTVGETGRGVMLTGMKPVGAGQELTLQTARGEYKVTLPLIGRFQVSNALIAAGLAIATGADLQTAVTALSGIKGAPGRLELAGTTKSGGLVFIDYAHKPDALDNALAALRPFAKGALSVVVGAGGDRDPGKRVLMGQAAARQADVVIVTDDNPRSENPAKIRKAVLQGAPDALEIGDRYEAIAEGVRRLGKGDILCVAGKGHETGQIVGDRVLPFSDHEEVRRALGL",
    "response#2": "MKLSELLKGITYQEVKGDMDTEEISGLTLDSRQVKSQGLFLAVKGAQLDGRDYVRDAVSRGAAAILADADEEDLPVAWQADIPFIPVFQLRKQLGGLAARFYGNPSRQLFLKGIGITGTNGKTTTSYLTKSIIEASGKKTGLIGTIQINVGGEKIKSGLTTPESTDLQETLKMMKEAECEYVVMEVSSHSLELDRVYGCEYDTAVFTNLTQDHLDYHKTMENYYNAKARLFSQLGNKKNSIINIDDEYGKRLAEEGDAEIITYAVKNADYRATDLTVSAEGVEFTLVTPYGKCSIRTKLIGKFNVYNVLAAISAGLCEGISLETIKEALENIEGVSMGRFEYVVSNNGVTAIVDYAHTPDALKNVLETINDIRTGNEKVITIVGCGGDRDKTKRPKMAHIASQLSNQAIFTSDNPRTENPQTILDEMEKGVSAENYKKTLTVLNRKQAIKTACKFSKNGDIILIAGKGHETYQIIGTEKFHFDDREVAREAIRGRIK",
    "response#3": "MKKISGLTADSRKVRPGFLFAAFKGASVDGTRFAPDAAAAGAVAVLCADDTMDSVRAACPQHMIVIGAPEPRQVFARMAARFYGHPSRELRMIAVTGTNGKSSVANFYYQILKNLGYKVGLIGTIYSRIGSEEIPAERTTPESLELQKLFREMREQHCEYVVMEVSSHALDLFRVDGCEFSAALFTNLSQDHLDFHGSMEEYLSAKLRLFTGLRPKVAVVNRDDPWGSRLVEEGAGSVKTIGFSLGGEVRATNLRFSSRGTSFLVEFNGESFDCDLHLRGRFNVYNALAALACALAAGVPLEEAVRSLKLVPSVPGRMELFQVANKPKIVVDYAHTPDALEKALQALRVHCEGKLWVIVGCGGDRDKGKRPMMASIAEQLADYVVLSDDNPRGEMPSAIVDDMLAGMNNPAAAMIEHSRFEAAKIALAQAGTSDIILLAGKGHETYQEIKGEKRHFSDLEVARECLRSLEGRT"
  },
  {
    "reference": "MPINETASVTNCDTNFEPLIENLKLFLQLCYLTPSALFLSRVIYITAWKYRKKFRKQRFYTIFLADCVTGFILVNFSIFFTRPLIYVPQACEFVLEHIKKLALFLDIYYPCFRYLQAFQILVQILFVANRASCVLWPLSYSLFWKKWLKSILTTMAISPCLWIWTIAISDKMIVHGYGGLVVLYYRYVSWARSTLFFSILRLTSVITIVVATTTMLIKMSRMKKRIRESERRLCWASVYLSVCYLLPAIAEFEYFLVLKAKLFENSGILHGLVVICWDIQNICSTYVM",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein can be targeted to membrane and perform transmembrane signaling receptor activity.2. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "GRTANGKVRLYVIGQGALSAAVAANSKHDIAEAERWNLPIILIVLLAVFGSLAAAAVPLALGVCTVVVTMGLVDLVSMHTIMSVFVTSTVSMFGIALAVDYSLFILMRFREELRSGRQPQEAVDAAMATSGLAVVLSGMTVIASLTGIYLINTAALKSMATGAILAVAIAMLASITLTPAALATFGRAAVKRSVLMHWSQRSECTQSLFWTRWVGWVMHRR",
    "response#2": "DLEKRIFRGYDIKKRPVKNASVPTVVDVHWHVIHVSINQKEQTMTLHGHIYMRWYDEYLVWDPKDFAGIHYARVKKWQVWQPKIRVSNSASGLASAFDFSTSAHVIIQMVEKDRAKVEMYPTFSIKVGCMFDFGDFPYDQNKCSVNLFATDTMAEVQLQ",
    "response#3": "DLEKRIFRGYDIKKRPVKNASVPTVVDVHWHVIHVSINQKEQTMTLHGHIYMRWYDEYLVWDPKDFAGIHYARVKKWQVWQPKIRVSNSASGLASAFDFSTSAHVIIQMVEKDRAKVEMYPTFSIKVGCMFDFGDFPYDQNKCSVNLFATDTMAEVQLL"
  },
  {
    "reference": "MTIVILAAGQGKRMNSDLPKVLQPLGGRPLLAHVIETARALSPEAINIVYGHGAEDVRTTFTDADLTWSLQAEQLGTGHAVAQALPSIPTDHSVLVLCGDAPLVRPDSLSALIDIVPQGSLGLLTARPADPSGYGRVVRDDAGIVRGVVEERDANEAEWSIDEINTGVMVLPADWLGQALSALGNDNAQGEYYLTDVIGLAVEQELPIVTVEAEDASEMLGINDRSQLAEAERITQERHAQRLLDQGATLADPNRFDLRGTLTVGRDVFIDVGAVFEGDVKLGDRVRIGPNAIVSNSTLGDDCIVHAHCVVDGIAAGPRCEIGPFARLRPGTNFAEQVKVGNFVEVKATEVAAGSKMNHLTYVGDSSVGRNVNIGAGTITCNYDGANKNRTIIGDRVFVGSGVMLVAPVTVGEGATIGAGSTIGRDVPAEALTVERAKPKTVPDWKRPEKKK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design must be focused on enhancing the efficiency of regulation of cell shape. The designed protein sequence is ",
    "response#1": "KEQFFHEEECGDLVTRAVLVDMEPKVVSKTLSMAGRSGKWKYDISSQFSQKQGCGNNWANGYCVHGPKHQDIVMDLVRKQVEKCDRLGGFFTIMSMAGGTGSGMGTFLTRCLRDAYPDSLLLNQVIWPYGTGEVIVQNYNSILTLSHLYRSSDALLVHENDIIHKVCSQLMNIKQISFRDVNKVIAHQLGSIFQPVYTSC",
    "response#2": "KEQFFHEEECGDLVTRAVLVDMEPKVVSKTLSMAGRSGKWKYDISSQFSQKQGCGNNWANGYCVHGPKHQDIVMDLVRKQVEKCDRLGGFFTIMSMAGGTGSGMGTFLTRCLRDAYPDSLLLNQVIWPYGTGEVIVQNYNSILTLSHLYRSSDALLVHENDIIHKVCSQLMNIKQISFRDVNKVIAHQLGSIFQPVYTSC",
    "response#3": "KEQFFHEEECGDLVTRAVLVDMEPKVVSKTLSMAGRSGKWKYDISSQFSQKQGCGNNWANGYCVHGPKHQDIVMDLVRKQVEKCDRLGGFFTIMSMAGGTGSGMGTFLTRCLRDAYPDSLLLNQVIWPYGTGEVIVQNYNSILTLSHLYRSSDALLVHENDIIHKVCSQLMNIKQISFRDVNKVIAHQLGSIFQPVYTSC"
  },
  {
    "reference": "MRQKFIFITGGVVSSLGKGIASASIGALLEARGLTVMLKKLDPYINVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFTNAIGSKRSNFTTGQVYDAVISRERKGEYLGVTVQVIPHITDEIKQRILDVDEGVDIVIVEIGGTVGDIESLPFLEAIRQLRNDLGKDNTLFIHLTLVPFIKSADEVKTKPTQHSVKELREIGIQPDILICRSESFLTQPIKDKIALFCNVEKDAVITAKDVDIIYEVPILFHKEGLDEKITRLLNIWSRKPDLSKWEKIVNTIKSPKRQVEIALVGKYVRLKDSYKSLNEALVHGGIANNARVNIKYVDSEEIESGTLNQLEGVDGILIPGGFGDRGIEGKIAAIKYAREHKIPFFGLCLGMQLAVIEISRNLAGLKDAHSTEFNENTPYPVIYLIEEWVDRENRLQKRDKFSNKGGTMRLGAYKCILKQGSLAHKAYMQDVVFERHRHRYEFNMAFRKPLEDCGMEITGISPDGKLAEIIELKGHPWFLACQFHPEFKSKPFDPHPLFKEFIKASLKHRQEKSKWKKK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The active site, Nucleophile; for glutamine hydrolysis of the protein must be engineered to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).2. The protein design should consider the endogenous proteins and enzymes present in the CTP from UDP: step 2/2, Pyrimidine metabolism, CTP biosynthesis via de novo pathway pathway to avoid interference with native metabolic processes.3. The protein should be able to bind L-glutamine, ATP, UTP ligand in a variety of conditions4. The designed protein must possess CTP synthase activity, metal ion binding, ATP binding to support 'de novo' CTP biosynthetic process under certain conditions.5. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. The designed protein sequence is ",
    "response#1": "IFVTGGVVSSLGKGIASASLAAILEARGLRVTMMKLDPYINVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFVRTRLTGKNSITTGKIYEAVIRKERRGDYLGATVQVIPHITDEIKHSIFEATRGFDVALVEIGGTVGDIESQPFLEAIRQIRIEHGADKVMFMHLTLVPYIKAAGEIKTKPTQHSVKELRALGIQPDVLLCRCETPLPDSERRKIALFTNVPEKAVISAVDVDTIYAIPRWLHKQGLDELAVAHLKLDVPAADMADWRTVVDRVTHPEGEVRIAIVGKYIEIKDAYLSIKEALKHAGIDSGCLVDIKWVEAEELEKMDIDEVREVFSDVEGILVPGGFGTRGSEGKIKAIRYARENKIPFLGLCFGFQLAVVEFARHVLGLEEAASAEVSETPYPVIEILPEQRNVRNMGGTMRLGDAEITLEEGTRAYEIYGKSKIIERHRHRYEVNPRYIEAIEKKGMVFSGKCGNRMEIAEIPDHPFFIGTQFHPEFKSRPDNPSPVFRAFIRAAHDRAKAREAAA",
    "response#2": "KYIFVTGGVVSGLGKGITAASLGRLLKARGLKVAAQKLDPYINVDPGTMSPYQHGEVFVTNDGAETDLDLGHYERFIDEDLNKYSNLTTGKVYWNVINKERSGEYLGETVQVIPHITNEIKSFVYEVGKKTDADVVITEIGGTTGDIESQPFIEAIRQISLEVGLSNCLFVHVTLIPYIQSSGEHKSKPTQHSVKELRSIGIMPNIIIARCDEPIDDSIRHKIAMFCNVKLDCVIQNITLPVLYEAPIMLDKENFSDIVCRELSLQFRKTDWEEWKVLVKKIKLQDSYKSVYEALRHAAIANKVELNIMKIDSEEIEKNPENLTKVINQADGILVPGGFGSRGIEGKLAIIQIAREKKIPYFGICLGMQCMVIEYARNVLKIADANSSEFTETSNPVIHLMEDQYSIDRKGGTMRLGVYECNIKKGTLAEKIYGENIIFERHRHRYEFNNKYEDQFEKAGLIISGKYITKNLVEIVEYKDNPFGIGVQFHPEFKSKPFKPHPIFKSFIDLIT",
    "response#3": "IFVTGGVVSSLGKGIASASLAAILEARGLRVTMMKLDPYINVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFVRTRLTGKNSITTGKIYEAVIRKERRGDYLGATVQVIPHITDEIKHSIFEATRGFDVALVEIGGTVGDIESQPFLEAIRQIRIEHGADKVMFMHLTLVPYIKAAGEIKTKPTQHSVKELRALGIQPDVLLCRCETPLPDSERRKIALFTNVPEKAVISAVDVDTIYAIPRWLHKQGLDELAVAHLKLDVPEPDLSEWSEIISRIKNPEGEVRIAIVGKYIEIKDAYLSIKEALKHAGIDSGCLVDIKWVEAEELEKMDIDEVREVFSDVEGILVPGGFGTRGSEGKIKAIRYARENKIPFLGLCFGFQLAVVEFARHVLGLEEAASAEVSETPYPVIEILPEQRNVRNMGGTMRLGDAEITLEEGTRAYEIYGKSKIIERHRHRYEVNPRYIEAIEKKGMVFSGKCGNRMEIAEIPDHPFFIGTQFHPEFKSRPDNPSPVFRAFVGAALKHARGK"
  },
  {
    "reference": "MKQADELRFNEDGLIPAIVQDAASKEVLTLAYMNKESYEKTLETKETWFYSRSRQALWHKGETSGNTQAVKGIRYDCDQDALLVLVEPSGPACHTGSYSCFTKEQTEEQAADRFGIMNELERVIAERQAEMPEGAYTTYLFREGVDKILKKVGEEASEVIIAAKNRDHEELKWEAADLLYHLLVLLREQSLPLDDVLDVLKKRHSEIEE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be optimized for phosphoribosyl-ATP diphosphatase activity. The designed protein sequence is ",
    "response#1": "RDDDIPGLVMDKVVDLGIVGENVLEETMLERQSQGLPTSYKKLKDLDYGGCRLSLAIPREEEWTGVAALQGKKIATTYPHLLKRFLAEQGVTFKPVLLTGSVEVAPRAGLAEAICDLVSSGATLEANGLKEVQVIYRSKASLIQADTELYPEKQKLVDRLMPRLF",
    "response#2": "PEKQKLVDRLMPRLEGMLQARESKYIMMHAPKDKLAEITALLPGAENPTVMPLASDSTHVAMHVVATETLFWETMEKLKALGASSILVLPIEKMVG",
    "response#3": "RDDDIPGLVMDKVVDLGIVGENVLEETMLERQSQGLPTSYKKLKDLDYGGCRLSLAIPREEEWTGVAALQGKKIATTYPHLLKRFLAEQGVTFKPVLLTGSVEVAPRAGLAEAICDLVSSGATLEANGLKEVQVIYRSKASLIQADTELYPEKQKLVDRLMPRLL"
  },
  {
    "reference": "MLAVCCALLTALLAAPGAALAQGGCPALEVASDVLTRLPGASVVLTCPGGEPGDNVTFHWMLRSRVIGGPGSHRDRWTVVGRRLVLSSVQLSDSGNYSCYQDGRLVGTVCLLVDVPPEQPQLSCSRKSPLSNVGCEWSPQRPPSPTTKAVLLVKKFQNSPVENFQEPCLYSQESQKFSCQLAVPEGDNSFYIVSLCVANSAGSESSRSQTFEGYGILQPDPPVNITVTAVDGNPRWLSVTWQDPPSWNSYFYRLHFELRYRAERSKTFTMWMVKELQHHCIIRDAWRGMRHVVQLRAQEEFGHGLWSEWSQEVMGTPWTEPRSPEVETEVSPSTQASTTNEDNENTLFKDSANATSLPVQDSSLTSLPTFLVAGGSLAFGTLLCIGIVLRFKRTWKLQALKEGKTGMHPPYSLGQLVPERPKPTPVLVPLISPPVSPSSPESDNTPRHSRPDARGPQSPYDISNRDYFFPR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have enzyme binding and be active in apical plasma membrane.2. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.3. The designed protein should contain one or more Fibronectin type-III, Ig-like domains that are essential for its function. The designed protein sequence is ",
    "response#1": "PKTVNVELGETVQLTCHSPCKFYSYKKFWCKWTDQGCSALPSQDEGSGQAVVNCDQNSQLINLTLKQVTKGDEGWYWCGVKEGLQYKETVAVYVK",
    "response#2": "VHSTVLLPCENHDHMWTDRHREEDQQVVHWDRQAPGIPHDRADRLIDMYASGERRAYGSLFLRRKMNVSNSAFSQGDFTLFIPYLTRGDEGTYSCHLHHHYM",
    "response#3": "VHSTVLLPCENHDHMWTDRHREEDQQVVHWDRQAPGIPHDRADRLIDMYASGERRAYGSLFLRRKMNVSNSAFSQGDFTLFIPYLTRGDEGTYSCHLHHHYH"
  },
  {
    "reference": "MRLHHLLETAAIPVPALVADREIRAVSGDSRRLPPAALFVGVPGAHVDGGDYWAASLAVGAAVAVIGPRAARKHPPGPADPVVVVEDPAACLGRLASAFWGHPSRHLQLIGVTGTNGKTTTTHWIEHLSTALGTPTALLGTLENRWPGTVMTASHTTAPADMVHGHLAQAVADGVQRAAMEVSSHALAQQRAAGLAFAGAVFTNLSQDHLDFHGDMESYFQAKARLFAADMLHGQAVINCEDHHGRRLHRELGSKAWGCRLLDPRQRDQGPPLDRNGELVMCDLAMGAKGVEGRLISPMGEGRFRSPMLGRFNLMNLLQAVGALLSCGAPLGPLLEHVPRFGGVPGRMESIGAPDLTVVVDYAHTPDGLTSALQALRPFVAGRLICVFGCGGERDRGKRPLMGAAAAELADELVITSDNPRTEDPEGILEEICAGLPTGIPHHRELDRRLAIHRAVVQAVAGDTVLIAGKGHERVQIIGHEQRPFDDRRVAAAAIAER",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Mg(2+) is required for the proper folding and stability of the protein.2. The protein design must feature the Meso-diaminopimelate recognition motif motif.3. A protein that can perform magnesium ion binding, ATP binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity in cytoplasm to support peptidoglycan biosynthetic process, cell division, cell wall organization, regulation of cell shape, cell cycle.4. The designed protein must have at least one Mur ligase central, Mur ligase N-terminal catalytic domain.5. The ATP, meso-2,6-diaminoheptanedioate binding site should be stable and able to withstand changes in environmental conditions.6. The protein should be optimized for activity in Cell wall biogenesis, peptidoglycan biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.7. We are looking for a protein with broad substrate specificity for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "MESLSVDARNIEPNGLFLAVSGSAAHGLIYAQQAIDAGVAAIVYETKEDVEFLVEKLKKQSSVRLIELPNLSAHISEIAARFVSNLELDSRAIEAGDTFVAIIGHEVDGRRFIDNALAAGATSVIAQTADESQHGQVDTSGASPVIYLYDLGRFLSELAADLYQSQPSRTIAVTGTNGKTSVAAFVRQIWEALGLSAVNIGTTGVEGAWTAPSPHTTPEPITLHRMLAEMAQAGVTHAAMEASSHGLDQRRLEAVRLAAAGFTNLTQDHLDYHGDMDSYFAAKAGLFDRLLPGDGVAVVNLDDPRGPDLAAACEARGQEVIGVGRNEAARLRLTGQRFDATGQEVLFRWQGRARSVRLDLIGGFQAANALIAAGLAVTAGVEPETALQGLARVGNVPGRMERAALLPNGACAYVDYAHTPDALARLLTSLRPHARGRLVLVFGAGGDRDSGKRALMGQEAAQGADIAIITDDNPRTENPATIRAAIRTGAPNALEIPDRRTAIADALALLEPDDVLVVAGKGHETTQTIGSKILPFDDRDVARDALRKRLLL",
    "response#2": "MQLTTLLKKIPFKIIMGNEDTSLSINSQTVERGALFIAIPGSQHDGTAFIDEALRRGAVGIVADPRQALGQLAAGYYGHPTAQLQLVGITGTNGKTTTAAAIYSTLLDLNKSVFLCGTRGAFVNEKVVDEKSLTTSPPLKMLSYLQTATKEKCEYFIMEVSSHAIAQNRIEGLNFALKVFTNLSQDHLDYHKTLQEYYAVKSSFFMDESLKLINKDDENIQFNYKNAYTYSLNNPATFSTLAYSLNNGIDAILRTMDAEAEISSSMVGEFNLYNLIAAFCAGVALGIPTEILLQGIANCTGVPGRMQRVPSNEGCFIVDYAHTPDALQQVLASLKTHCDGQLWAVFGCGGDRDAGKRPLMAQAGLAGADQIILTADNPRTEDPNIILQDMQAGMSAEQYQRTHIEPARQKAIEYAVRQAAAADIVVIAGKGHEDYQIIGHTKHHFDDREVAREALGK",
    "response#3": "MTLAELIAGLAVDNRRAGPGDLFAALPGSVTHGARFAPDAVERGAVAILTDRAGAATLENLSVPVLTVEDPRAALAFAASLSVDARNIEPNGLFLAVSGSAAHGLIYAQQAIDAGVAAIVYETKEDVEFLVEKLKKQSSVRLIELPNLSAHISEIAARFFGVTGTNGKTTITYLIEAILKSAGYTPAVFGTVDYRLADTLRFEATHTTPESLDLMRMLAEFRRHGANALVMEVSSHALEQHRADGLDFDIAVFTNLTQDHLDYHAGLEPYFASKRRLFTELLGNGVALINADDPYGRRLLTDIPAARTFGRRSSCTLYPRQVTIGREGIHGVFSGSHGEVQIDSPMIGEFNLSNLLAAVAVALECDSRQVKPGALFFALPGSKTDGRKYISQALAAGAIGVVADSFAGPEQAEGCCFIQVANVRRAMAQAAAHYGIAAERVEAGLAHLRGVPGRMERAALLPNGACAYVDYAHTPDALARLLTSLRPHARGRLVLVFGAGGDRDSGKRALMGQEAAQGADIAIITDDNPRTENPATIRAAIRTGAPNALEIPDRRTAIADALALLEPDDVLVVAGKGHEDYQEVAGVRHPFDDRDVAREALVGRGAA"
  },
  {
    "reference": "MTRTAVGTFGAPDGAGPEFDVYSGNANRELAAKIGRYLDRPLGRAEVFEFANQNIFVKILDNVREKDVYLVQPTSHPVNRSIMELLIMIDAFKRASAGRITAVVPYYAYGRSDKKDQPRVPITARLIADMITVAGADRLLTVDLHQGQIQGFFNIPVDELTAVHLIGNYFRQKQLEDMVVVTDLGFAKRARSFAELLDAPLAVIEKRRVGNLDRAELLNVIGEVRGRRAIVVDDEIDTGGTLMEVVRALQREGVEEIYACATHGILSDPAIDRLRESPIKEIVITDTVPLPAAKRLSRIKVLSVAPLIGEAIRRIHYGESVGALFSSELDLVQEMLLWDDSDAGEAPPSGGLFGQPPSNQPAVQQPAGIEPASTEPARAGA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The active site active site of the designed protein must be optimized for substrate binding and specificity.2. For general function, the protein need meet that Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P).3. A protein with ATP binding, magnesium ion binding, ribose phosphate diphosphokinase activity is needed to enhance ribonucleoside monophosphate biosynthetic process, nucleotide biosynthetic process, phosphorylation in a specific context.4. Ideal protein should have a binding affinity for key enzymes in the 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, Metabolic intermediate biosynthesis, 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1 pathway to enhance the overall efficiency of the pathway.5. The ATP, Mg(2+), D-ribose 5-phosphate binding site should be stable and able to withstand changes in environmental conditions.6. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "MKLMSGNANRPLARAIADYLDAPLSNVEIETFADGELFVRIEENMRGEDVFLIQSTSKPANNHLMELLICMDALSRASARRITAVIPYFGYARQDRKTGGRTPITAKLVANLIAKAEEASDLHAGQIQGFFNCPVEKIAPVLVRDIQARLKLDNVMVVSPDVGGVVRARALAKRIDAPLAIVDKRRERAGESEVMNIIGDVDGKICILIDDIVDSGGTLCNAADALRAQGASDVYAYITHGVLSGGAAAGALAEANVAAAE",
    "response#2": "MKILSGNSNRPLTEAIAAYLGLPLTKCQVRRFADMEIFVEIQENVRGQDTFIVQSTSYPTNDHLMEMLIMTDALRRASARRITAVLPYFGYARQDRKPGPRTPISAKLVANLITRAAPVLVDYLATRDLGEVVVVSPDVGGVARARAFAKRMDDAPLAIIDKRRSAHNVAESLTVIGDVAGKTAIVVDDMIXTGGTISKGAKLLRQQGAERVIACATHAVFSPPAVERLSALPLLAEYVERTYSGQPLTVVAPDSGRVRVAERWTDRLGGCPLAFIHKTRDVTTPGQVVANRVVGEVAGRVCVVVDDEIQTGGTVCKAADLLFAAGAAEVIVVVTHPVLPDPAVERLKNSRISEVVVTNGCTVIYAPAAPVLFEYFRDLKKRLETRNITIVAPDVGRSKVARGYAKRLNAGLAIVDKRRVSGEETEFLDMIGNVEGQVCVIIDDMIATGGSIVDAAKACKKFGAGEIYVACTHPVMCGPAVERLSNAPLQECVVCDATLQVPESIKLKVFTGRAHPDLAREVCSYLSIPLGQAEIKTFSNENIFVRINESVREADVFVIQPSCSPVNDGLMELLIMIDALKRASAARICAVMPYYPYARSDKKDQPRVPITARLVADLLTVAEMAPSIQVPQAVSAH",
    "response#3": "MKLMSGNANRPLARAIADYLDAPLSNVEIETFADGELFVRIEENMRGEDVFLIQSTSKPANNHLMELLICMDALSRASARRITAVIPYFGYARQDRKTGGRTPITAKLVANLIAKAETFYGQPSQRLKVIGVTGTNGKTSTSQMIASMLAAAGKRVGLIGTIENIIGNETIPSKNTTPEAPDLHSLFAKMREKGCEYCVMEVSSHALALHRVDGCEYDAAIFTNLSQDHLDFHKTMDNYFAAKAKLFTESPVLLDYLTSKQLHDIVVVSPDVGGVARARAFAKKLNDAPLAIIDKRRQAHNVAEVLNVIGDVKGKTAVLVDDMIDTGGTIAAGAKLLREEGARQVYACATHAVFSPPAVERLSSGLFEEVIVTNSDEGAKRALAAIAGRKVVISGRSHPELAKKVAEELATSLLPVDARTFASGEIYTRMEESVRGCDAFVIQSYSGPVNEWLIEQLIMIDTLKRASAKRITAVVPYYPYSRQDKKSRGRDPISARLVADLFKTAGASFELQLGEERIEVKSPGAFSIYNALAAAACAYCAGVSPETIAAGLERVKGVP"
  },
  {
    "reference": "MPLLHRTIIFLQLLGTISSNYNAFFAIMVTNPTVVQQVNQIEVNLTQSNPQFVKFVHNISHLHIKLNTVELNETRLPIVQKFLKELPKAMCNVSYSIPALFEGTAVMANTTLYGKVNPMSSSVISSVHQKIFSLLNNTEIPSVDVHDIFHPRMNIVELDNRKHEDLLQIMSNRTGDFKLNTGDFIKEIVLFNSTESGSFHEEIGRAVLAPCSNFTLLRFKKPELISNRVPIFPLEMDFK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be expressed specifically in the extracellular region2. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "MCCGSEAYDANSQICCNGNINTKATGPTAQPGCCGEFSYDAASQLCCDSHPVLMVGSLPSCCGRNGYDANTSLCCGDNNVAFVSGPQAACCGDMGYNRNTHLCCDSNVLPMPAMGACCGSWTYSQQTHLCCEE",
    "response#2": "MCCGSEAYDANSQICCNGNINTKATGPTAQPGCCGEFSYDAASQLCCDSHPVLMVGSLPSCCGRNGYDANTSLCCGDNNVAFVSGPQAACCGDMGYNRNTHLCCDSNVLPMPAMGACCGSWTYSQQTHLCCEQ",
    "response#3": "CGGVLYNPAAEMCCHGNVEPRVGASPMCCESSSYDPSTQMCCEGTVSNKPPGIAMCCGSEAYDANSQICCNGNINTKATGPTAQPGCCGEFSYDAASQLCCDSHPVLMVGSLPSCCGRNGYDANTSLCCS"
  },
  {
    "reference": "MFEDKPHAEGAAVVAAAGEALQALCQELNLDEGSAAEALDDFTAIRGNYSLEGEVTHWLACSLYVACRKSIIPTVGKGIMEGNCVSLTRILRSAKLSLIQFFSKMKKWMDMSNLPQEFRERIERLERNFEVSTVIFKKYEPIFLDIFQNPYEEPPKLPRSRKQRRIPCSVKDLFNFCWTLFVYTKGNFRMIGDDLVNSYHLLLCCLDLIFANAIMCPNRQDLLNPSFKGLPSDFHTADFTASEEPPCIIAVLCELHDGLLVEAKGIKEHYFKPYISKLFDRKILKGECLLDLSSFTDNSKAVNKEYEEYVLTVGDFDERIFLGADAEEEIGTPRKFTRDTPLGKLTAQANVEYNLQQHFEKKRSFAPSTPLTGRRYLREKEAVITPVASATQSVSRLQSIVAGLKNAPSDQLINIFESCVRNPVENIMKILKGIGETFCQHYTQSTDEQPGSHIDFAVNRLKLAEILYYKILETVMVQETRRLHGMDMSVLLEQDIFHRSLMACCLEIVLFAYSSPRTFPWIIEVLNLQPFYFYKVIEVVIRSEEGLSRDMVKHLNSIEEQILESLAWSHDSALWEALQVSANKVPTCEEVIFPNNFETGNGGNVQGHLPLMPMSPLMHPRVKEVRTDSGSLRRDMQPLSPISVHERYSSPTAGSAKRRLFGEDPPKEMLMDKIITEGTKLKIAPSSSITAENVSILPGQTLLTMATAPVTGTTGHKVTIPLHGVANDAGEITLIPLSMNTNQESKVKSPVSLTAHSLIGASPKQTNL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design must be focused on enhancing the efficiency of negative regulation of G1/S transition of mitotic cell cycle.2. For general function, the protein need meet that Key regulator of entry into cell division; Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression  The designed protein sequence is ",
    "response#1": "EDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRSCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALECEVCQQGGEIILCDTCPRAYHLVCLDPELEKAPEGKWSCPHD",
    "response#2": "FEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELLGCNAAILKRETMRPSSRVALMVLCEAHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIE",
    "response#3": "FRPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEEGTIHLYIYSIQSIPKGTEITIAFDFDYGNC"
  },
  {
    "reference": "MNSLSNKKILVVICGGISAYKSLELIRGLKKKQVEIKTIITKSAKEFVTPLSITSLSQGKVYEDLFSSESESEMDHISLSRWADLIVVVPATANTISKLSNGLTDDLASTVLLASNKKIFIVPAMNVRMWDHPSNKKNIIKLREYGYSIIGPEIGDMACGEYGEGKMTEPSDILLHIENYLSSLQKNKKYKALVTAGPTREYLDPVRYLTNKSSGKQGYEIAKSFRDNGFETTLISGITNLKPLEDIKFIEVETADEMLKKTIENLPVDVAVFSAAVCDYKFKKYNKEKIKKNAELDISMIKNTDILSYVSKHNSLRPKLTVGFAAETENLDLNAKDKLSSKNCDWIIANNVSDNEIGFDSEYNEVKIFYKNNKNEILHKMNKSLIAENIVERVIKNLN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal domain.2. A key design requirement for the protein is the optimization of the Proton donor to achieve high catalytic efficiency for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.3. The CTP binding site should be located in a region of the protein that is accessible to the ligand.4. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..5. A protein should be able to manipulate the activity of enzymes in the coenzyme A biosynthesis pathway, either by activating or inhibiting their function.6. Mg(2+) is required for the proper folding and stability of the protein.7. The designed protein must possess metal ion binding, FMN binding, phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity to support coenzyme A biosynthetic process, pantothenate catabolic process under certain conditions. The designed protein sequence is ",
    "response#1": "MNIVVGITGGIAAYKAVGVVRGFVLAGHSVQVIATEHALRFVGKPTLEAISRNTVHTDLYEGVAEVRHVALGQAADLVVIAPTTANTLAKLAAGLADDLLGNTVLATTAPILLAPAMHTEMWRNAATVANAALLALRGYRFIGPATGQLTGTDVGPGRMSEPDEIVAQAEAIVMSGKHVLITLGPTREKWDAVRFWSNPSSGLMGACIAMAAWLRGAKVTVVSGPVNWWFPEDINVIKVNSAQQMFDAATEVWPSCTTGCFTAAVADFKPIPHGEGKFKKEGSSALRVDFDTNPDILKTIGSMKKDDQQLIGFAAETSNIQEAAKGKLERKNLDLIVANPINKPGAGFESSTNSVYLEFLDELTRPVLEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLDNLNGNVKISLEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLAVFVE",
    "response#2": "MNIVVGITGGIAAYKAVGVVRGFVLAGHSVQVIATEHALRFVGKPTLEAISRNTVHTDLYEGVAEVRHVALGQAADLVVIAPTTANTLAKLAAGLADDLLGNTVLATTAPILLAPAMHTEMWRNAATVANAALLALRGYRFIGPATGQLTGTDVGPGRMSEPDEIVAVLQDPEGLEKHIVLGLSGGTACYKSAELCRLLVKAGASVQVVMTEAATQFITPVTMQALSNRPVLLSQWDASEPNNMPHIRLGREPDAILVAPASADLMAQLAQGRAADLLTLTCLARPLDRTPLLLAPAMNREMWAHPATQRNAAQLRADGALVLPVGQGDQACGEVGDGRMLEPQELLEELIAAAEALLEPARLSGRHFLLTAGPTREELDPVRYISNHSSGKMGWGIAQGALRAGAQVTLVHGPVNLPSIPGALAIKVTSAREMYAQVMGLWETHTATQTLDAVILTAAVADFRPVVKSPEKIKKTAATTLNVELEANPDILLEIGRRSQSLVNGKRPFLVGFAAETGSALERGREKMARKKCDILVVNDLFEEGAGFEGETNRVTLLTPDGLEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLDMAGRKNSILEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVL",
    "response#3": "MNIVVGITGGIAAYKAVGVVRGFVLAGHSVQVIATEHALRFVGKPTLEAISRNTVHTDLYEGVAEVRHVALGQAADLVVIAPTTANTLAKLAAGLADDLLGNTVLATTAPILLAPAMHTEMWRNAATVANAALLALRGYRFIGPATGQLTGTDVGPGRMSEPDEIVQMARQLVLEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLSAVEANGEPVLEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLESIALDGAPGVLGLEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLEAVALDGQPVRLLEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVL"
  },
  {
    "reference": "MAFKQLLAALSVALTLQVTQAAPNLDKRVACPDGVHTASNAACCAWFPVLDDIQQNLFHGGQCGAEAHEALRMVFHDSIAISPKLQSQGKFGGGGADGSIITFSSIETTYHPNIGLDEVVAIQKPFIAKHGVTRGDFIAFAGAVGVSNCPGAPQMQFFLGRPEATQAAPDGLVPEPFHTIDQVLARMLDAGGFDEIETVWLLSAHSIAAANDVDPTISGLPFDSTPGQFDSQFFVETQLRGTAFPGKTGIQGTVMSPLKGEMRLQTDHLFARDSRTACEWQSFVNNQTKLQEDFQFIFTALSTLGHDMNAMTDCSEVIPAPKPVNFGPSFFPAGKTHADIEQACASTPFPTLITAPGPSASVARIPPPPSPN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a binding site that is specific to the target heme b, Ca(2+) ligand.2. The protein should be designed to have high stability and activity towards 2 (3,4-dimethoxyphenyl)methanol + H2O2 = 2 (3,4-   dimethoxyphenyl)methanol radical + 2 H2O.3. The protein's architecture should predominantly feature helix-helix-turn-helix-helix-strand-helix-helix-helix-helix-helix-helix-helix-helix-strand.4. The protein design should take into account the physiological conditions of the Secondary metabolite metabolism, lignin degradation pathway.5. For general function, the protein need meet that Depolymerization of lignin6. A protein with heme binding, diarylpropane peroxidase activity functions is required to enable hydrogen peroxide catabolic process. The designed protein sequence is ",
    "response#1": "QLREGFYEHSCPQAEKIIKDYVMEHIPHVPSIAATLLRTHFHDCFVRGCDASVLLNATGGNEAERDAAPNQTLRGNGFIDRVKALVEKECPGVVSCADILALTARDSVVATGGPSWSVPTGRRDGTVSIKQEALDQIPAPTMNFTELLQSFQNKSLDLADLVWLSGAHTIGIAHCNSFTERLYNFTGRGGPGDADPSLDPLYAANLRRTKCKTPTDNTTIVEMDPGSFRTFDLSYYRGVLKRRGLFQSDAALITDAAAKADILTAVNSPPEFFSQVFARSMVKMGAIEVKTGSEGEIRKHCAIVR",
    "response#2": "VMEHIPHVPSIAATLLRTHFHDCFVRGCDASVLLNATGGNEAERDAAPNQTLRGNGFIDRVKALVEKECPGVVSCADILALTARDSVVATGGPSWSVPTGRRDGTVSIKQEALDQIPAPTMNFTELLQSFQNKSLDLADLVWLSGAHTIGIAHCNSFTERLYNFTGRGGPGDADPSLDPLYAANLRRTKCKTPTDNTTIVEMDPGSFRTFDLSYYRGVLKRRGLFQSDAALITDAAAV",
    "response#3": "VRGADGSIMIFDEIETNFHPNIGLDEIVRLQKPFVQKHGVTPGDFIAFAGAVALSNCPGAPQMNFFTGRAPATQPAPDGLVPEPFHSVDQIIDRVFDAGEFDELELVWMLSAHSVAAANDIDPNIQGLPFDSTPGIFDSQFFVETQLAGTGFTGGSNNQGEVSSPLPGEMRLQSDFLIARDARTACEWQSFVNNQSKLVSDFQFIFLALTQLGQDPDAMTDCSAVVKWDPGSSLSFDSHYFEAIVKWHVIRALKANRRTGAALVRLLFHDCFVRGCDGSVLLDASYDNPHPEKEAPVNIGLAAFDLLEEIKAAVEDRCPDVVSCSDILIYAARDAASILSNGHVHFDVPAGRLDGFVSKAEEAQAELPDSADDVQKLIDNFARKNFTIEELVILSGAHSIGQGHCSSFTGRLSEPNDQITPAYRNLLKYKCAKGNPPVDNNVRDEDYDVVARFMPGFTSRVRKIPDFLDNSYYHNNLAKIVTFHSDWTLLTHKEAFGHVKEYAEVRGADGSIMIFDEIETNFHPNIGLDEIVRLQKPFVQKHGVTPGDFIAFAGAVALSNCPGAPQMNFFTGRAPATQPAPDGLVPEPFHSVDQIIDRVFDAGEFDELELVWMLSAHSVAAANDIDPNIQGLPFDSTPGIFDSQFFVETQLAGTGFTGGSNNQGEVSSPLPGEMRLQSDFLIARDARTACEWQSFVNNQSKLVSDFQFIFLALTQLGQDPDAMTDCSAVVKW"
  },
  {
    "reference": "MSEHTLILLGAGSSSRFVSDVKKQWIYTADTPLWLYVAEHFENSAQFDDIIIVSTLEEISAMKNFASYTYVQGGDSRQASLTNALTHVNSKYVLVSDIARCCVPTDMIKRILDAKDKASCIVPVLPVTDTLYLNNLPIDREKVRIIQTPQLSRTKLLKQALQTETLFTDDSSAIASLGEKIHFVXGSSKAHKLTTLEDLQKVTCLQGPSSKTLTGFGIDIHPFEDNKEMFLCGVPIDVDYGFKAHSDGDVAIHALIDALLGAAGLGDIGELYPDTEDAYAGADSKVLLLDTVTRIKSFGYTIXNIDLTIMAESPKLSPYKAQMRNTLCELLGIRKNLVNIKATTAEKLGFVGRKEGVTVHAIANLHYMNWKKL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. We are looking for a protein with broad substrate specificity for 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-   erythritol 2,4-cyclic diphosphate + CMP.2. The protein should be able to bind a divalent metal cation ligand in a variety of conditions3. The designed protein should contain one or more 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MVLNSVETWTGQGFDVHALVPGDHVMLCGVKIAHTRGLKGHSDADAGLHALTDALYGALGEGDIGHHFPPSDPKWRGANSAVFLRAAVALLKARGGKINNLDVTIICEEPKIGPHRETMRQAIATIAEIDRRRVSVKATTSERLGFAGRGEGIAALATATV",
    "response#2": "RTGSGFDVHAFASGDHVTLCGVKIPHDRALAGHSDADVGLHALTDAILGAIGEGDIGAHFPPGNPEWKGAASDLFLRDAARRVAALNGRIVNVDVTLICETPKIGPHREAMRTRIAQILQIGVERVSVKATTTEKLGFTGRGEGIAAQATAMR",
    "response#3": "VRTGNGYDVHQLEAGDGVTLCGIFIPHDQKLKGHSDADVALHALTDALLATCGAGDIGDHFPPSDMRWKGAESRIFLEHAAKVVHAAGGTIMNADISLIAEAPKIGPHRQAMREKLSEILGISLDRCSVKATTNETIGFVGRREGIAAIATATVVLK"
  },
  {
    "reference": "MLALDQPSGLDPSLGDLPGPCIQADRTACFGHRKICHGLRPAREACGAITVVPLPLPGCPEAGLALLEDPRLPGLPWDAHKRRAGHLAIRAGSLGMSGAAVLAAEGALRMGAGLVTLLVDPALRAEIAAQVPEALVCPWEGRVPEGVDALLVGPGGVTDVPDWLGPLVLDASALGLWEGPRWLERPGTVLTPHAGEWARLFGGEPPREPSSRLAAVRQASGPAIRVLKGAQTLVAGGNDPQVWVNPTGHSGLATGGSGDFLAGMVGALAARWGREDGRNGGVLRSHVAAAVWFHGAAADHLGAGPLRVRDLGPALSAVLRQRSGHA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be able to bind (6S)-NADPHX, AMP ligand in a variety of conditions2. The protein should have a fast turnover rate for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.3. The protein must exhibit the following characteristics: Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP; Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration..4. The designed protein should have NADHX epimerase activity, ATP binding, NADPHX epimerase activity to facilitate nicotinamide nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "MQELDRQTIQEHGIPGLELMEHAGHACMEAIFHEFGYTGGKRAVIFAGRGNNGGDGYVIARLLLQSGWQVRVCILAERERIGGDAAINLEQLPPAAVSYCTVPGQLAGLLQEELSQTDVIVDALFGTGLNSEVAGINREAVELINAAGCPILAVDIPSGIHGTSGRVLGCAVRATVTVTFAAAKLGHVLYPGADHTGRLVVADIGIPAELVATDDKYSGGVVGIYAGSEQFPGAGVLSCTGAVRTTNSMVRYVGGNACAITASLPEVVIHPDRFATGRVQARVLGPGRGTDRAAKEELVDLLDTVQPLILDADALTIISKDKDLVETLRKRLAPTVCTPHGGEFERLAKPVRELGHEVANSADDRLQAARDLAVALDCTVLLKGRFSVIANPRQTVVVDYGSSWAATAGSGDVLSGIVGAILARDASRRAFAHGYAWEVARPHLAAPGAADHKYTRGLVTVIGGAMPGASLLAAMAAQRAGAGYVVLTGEGSGGPHALVRRDEAEALADTRTGAVVIGPGLGRDAGARRRLRAALGAGHPLVIDADGLALLADGGLSHLRRLAVPAVLTPHEGEFRRLFGEPAGSKIDRARDAAARSGAVVVLKGADSVVAHPDGRVAVAAAAPAWLASAGTGDVLAGIMGAMIARGLDPFTAAQAALWLHGDAAERAGAGLIADDLPAHLAASLAVTADDVRSWYPWPGPRSDKYSRGVVGLDTGSLAYPGAALLGVDGALHTGAGMVRYAGPARESVLAAFPSVVAPLDPLAPGRVQAWVCGSGWPSADAARLEHRLRDRVPTVIDAGALTGLPATLPPGCLLTPHAGELARLLSVDRHDVEADPLAHARTAVRRTGATVLLKGATQYCVRPDGAAMIAEPGPAWSATAGSGDTLAGIAGALLAAGLDAWRAGAMAASLQARAAAILPGPRTPSEVAARGLPDAIVALR",
    "response#2": "MQELDRQTIQEHGIPGLELMEHAGHACMEAIFHEFGYTGGKRAVIFAGRGNNGGDGYVIARLLLQSGWQVRVCILAERERIGGDAAINLEQLPPAAVSYCTVPGQLAGLLQEELSQTDVIVDALFGTGLNSEVAGINREAVELINAAGCPILAVDIPSGIHGTSGRVLGCAVRATVTVTFAAAKLGHVLYPGADHTGRLVVADIGIPAELVKYSAGAVLVVGGAPGLTGAVCLAAEAAFRADAGYVAVATPPASFPVIEARLLEAVKAPLQRVDELAERATALAIGPGLGRGEEERALVRRLLAERTMPAVVDADALFELEPADWPAPRVLTPHSGELGRLLGEESRWVDAHRLEAVRRAADRYRCVVLLKGADTLVAAPGEGVLVSNSGTPALATAGTGDVLTGVVAAFLAKGVDARLAAAAAAFVHGRAAELVPHERGVVASDVIAVLAQVGRALPPRPRDSHKGHFGHVFVVAGSRGKSGAALMTGLAALRSGAGLVTLWLPESLQKHVIGKVPELMAEPIPDTPQGTSDAAGAEKILGQSSQANVLAIGPGMTTHPSTRTLIQELVRRSPVPVVLDADGINAFAGEVELLRNENGQPVVITPHPGEMARLLGSTISDVQNDRLGVSSRFSQSQKVFSVLKGFQTVVTTPAGLIFINSTGNPGMAKGGSGDILSGIMARFVGSWQRRFRGADMRALADHVSAAVYLHGLAGDLAAEEKGMESMIATDLLPHLPEAFKRG",
    "response#3": "MQELDRQTIQEHGIPGLELMEHAGHACMEAIFHEFGYTGGKRAVIFAGRGNNGGDGYVIARLLLQSGWQVRVCILAERERIGGDAAINLEQLPPAAVSYCTVPGQLAGLLQEELSQTDVIVDALFGTGLNSEVAGINREAVELINAAGCPILAVDIPSGIHGTSGRVLGCAVRATVTVTFAAAKLGHVLYPGADHTGRLVVADIGIPAELVAPHTNNVDKYSRGSVLVVAGSSRYPGAAVMAAKAAARAGAGYVTLAVPVTIAPLIQMQLPEVPVVGVAAEQDGTFSAAARPVILKLAERSSATLVGPGMRVTGGTVAVVSALINSKAPLVVDADGLNCLARLTNGHLDEFPEVTRRDAPLVLTPHRRELGRLVGLADTPPDSLTAALEAARRIVWADGGSELCIVAKGTATACVGVEAALLPEPGTASLATAGSGDVLGGIICALLARMPDDIEDLPLIAAFGCEVHAVAGSRAAKRYGSRGVMAGDIADEVTIATPHEAMAVYQAADPGNLIAEVEDGEGFGRLLDDKRRNAILVGPGSGLNERTRTSAVAALATGRPVVLDADAITVFTENPASLFDLIRGPVLLTPHEGEFRRLFPALSAVSGKVERTRRAARQSGATVLLKGPDTVIAAPDGRAVINVHAPATLATAGSGDVLAGMAAGLAARGLSPLAAGAAAAWLHGDCP"
  },
  {
    "reference": "MTAAPASPQQIRDRLLQAIDPQSNIRNMVAVLEVISSLEKYPITKEALEETRLGKLINDVRKKTKNEELAKRAKKLLRSWQKLIEPAHQHEAALRGLAGATGSANGGAHNCRPEVGAAGPPRSIHDLKSRNDLQRLPGQRLDRLGSRKRRGDQRDLGHPGPPPKVSKASHDPLVPNSSPLPTNGISGSPESFASSLDGSGHAGPEGSRLERDENDKHSGKIPVNAVRPHTSSPGLGKPPGPCLQPKASVLQQLDRVDETPGPPHPKGPPRCSFSPRNSRHEGSFARQQSLYAPKGSVPSPSPRPQALDATQVPSPLPLAQPSTPPVRRLELLPSAESPVCWLEQPESHQRLAGPGCKAGLSPAEPLLSRAGFSPDSSKADSDAASSGGSDSKKKKRYRPRDYTVNLDGQVAEAGVKPVRLKERKLTFDPMTRQIKPLTQKEPVRADSPVHMEQQSRTELDKQEAKASLQSPFEQTNWKELSRNEIIQSYLSRQSSLLSSSGAQTPGAHHFMSEYLKQEESTRQGARQLHVLVPQSPPTDLPGLTREVTQDDLDRIQASQWPGVNGCQDTQGNWYDWTQCISLDPHGDDGRLNILPYVCLD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Target a Polar residues compositional bias in the protein's composition for improved properties.2. For general function, the protein need meet that Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery; Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes; Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.3. A protein that can perform transcription coregulator activity, transcription coactivator activity in core mediator complex, nucleus, mediator complex, nucleoplasm to support positive regulation of transcription elongation by RNA polymerase II, RNA polymerase II preinitiation complex assembly, regulation of transcription by RNA polymerase II, positive regulation of gene expression, transcription initiation at RNA polymerase II promoter.4. The designed protein should contain one or more TFIIS N-terminal domains that are essential for its function.5. helix-helix-helix-turn-helix-helix-turn-helix-turn-helix-turn-strand-strand-strand is the desired arrangement for the engineered protein. The designed protein sequence is ",
    "response#1": "INVAVNPGASIVAFKWLNIEKPQIVNKAATRLAENPTTTSSNSSSSIYGYGINQYQPYGVCHPIPTKQACVALRKNGQFILFYQGEHKVEYHKICCNLTDNISIIEKASIGFNNDKQIIVTAWDSLSNDINVYSIDINWGFLVESAKRQKLDQHYHTPKEAQKPPRLTLKKLHQMKPIQCFKEEDGESQSQSVVPEMGKLKVEELSSIDIISSNPDPKSGLSILITYGSSIIYRYALEKSDSSNTTSGGETSKNPISQAFYNLGVEKKIDWKGDDSSVRLVFKDKLMRRGQIESIINGFLDLSSLIIYKDGTVDVVDTTSKQIVNNSKNNNSVTGDEMELDNNDYPPKLISNLFDQGFQFPKTSSKTKQSKSEEQQYLDKLKQLSKYIEPLRRMINKIDKNEDRKKDLSKMKSLLDILTDPSKRCPLKTLQKCEIALEKLKNDMAVPTPPPPPVPPTKQQYLCQPLLDAVLANIRSPVFNHSLYRTFVPAMIPSVLQGEVARLDPKFLVNLDPSHCSNNGTVHLICKLDDKDLPSVPPLELSVPADYPAQSPLWIDRQWQYDANPFLQSVHRCMTSRLLQLPDKHS",
    "response#2": "INVAVNPGASIVAFKWLNIEKPQIVNKAATRLAENPTTTSSNSSSSIYGYGINQYQPYGVCHPIPTKQACVALRKNGQFILFYQGEHKVEYHKICCNLTDNISIIEKASIGFNNDKQIIVTAWDSLSNDINVYSIDINWGFLVESAKRQKLDQHYHTPKEAQKPPRLTLKKLHQMKPIQCFKEEDGESQSQSVVPEMGKLKVEELSSIDIISSNPDPKSGLSILITYGSSIIYRYALEKSDSSNTTSGGETSKNPISQAFYNLGVEKKIDWKGDDSSVRLVFKDKLMRRGQIESIINGFLDLSSLIIYKDGTVDVVDTTSKQIVNNSKNNNSVTGDEMELDNNDYPPKLISNLFDQGFQFPKRRKDLRKPTLIIPSVLQGEVARLDPKFLVNLDPSHCSNNGTVHLICKLDDKDLPSVPPLELSVPADYPAQSPLWIDRQWQYDANPFLQSVHRCMTSRLLQLPDKHIPSVLQGEVARLDPKFLVNLDPSHCSNNGTVHLICKLDDKDLPSVPPLELSVPADYPAQSPLWIDRQWQYDANPFLQSVHRCMTSRLLQLPDKHL",
    "response#3": "INVAVNPGASIVAFKWLNIEKPQIVNKAATRLAENPTTTSSNSSSSIYGYGINQYQPYGVCHPIPTKQACVALRKNGQFILFYQGEHKVEYHKICCNLTDNISIIEKASIGFNNDKQIIVTAWDSLSNDINVYSIDINWGFLVESAKRQKLDQHYHTPKEAQKPPRLTLKKLHQMKPIQCFKEEDGESQSQSVVPEMGKLKVEELSSIDIISSNPDPKSGLSILITYGSSIIYRYALEKSDSSNTTSGGETSKNPISQAFYNLGVEKKIDWKGDDSSVRLVFKDKLMRRGQIESIINGFLDLSSLIIYKDGTVDVVDTTSKQIVNNSKNNNSVTGDEMELDNNDYPPKLISNLFDQGFQFPKIPSVLQGEVARLDPKFLVNLDPSHCSNNGTVHLICKLDDKDLPSVPPLELSVPADYPAQSPLWIDRQWQYDANPFLQSVHRCMTSRLLQLPDKHNI"
  },
  {
    "reference": "MAPSFDTLSEQDLHEEEVEIDFSDLKAQYEVRLEEGLDAFVVIDGLPVVPEESKPKLIKFLLKKLNTVGRTREDAIFMPLNEKGMSEGFAFVEYDTPEQAIAATKHLHGTPLDKKHTLAVNKLTDIDRYGREGRVDEEYTPPKIEPFQEKEHLRSWLGDPNARDQFAMYRGDKVGVFWNMKNNPPENVVDRDHWTQLFVQWSPLGTYLASVHPQGVQLWGGPQFGKQKQFPHPFVSLMEFSPGEKYLTTWSSRPIQLDGPPGALSYEEEGKNIIIWDITTGKPLRSFVSHELAAPAGPDGDAAQAKKKVQWPAFKWSADEKFVARMLPGQSISIYELPRMNLLDRTSVKIEGVMDFEWSPATVQREGVKQSEQLLSFWTPEIGSNPAKVGLMSIPSKEVVRTRNLFNVSDVKLHWQSQGAYVCVKVDRHSKSKKSLATNLEIFRVREKGVPVEVVDSLKDTVINFAWEPKGNRFVLITTGEVPTGTAVPPKTAVSFFAPEKTKSGAAGNFKLVRTIEKKTSNGIYWSPKGRFVVVATVHSQQHFDIDFWDLDFEGEKPENEKDLSANLQLMKTNEHFGVTDIDWDPTGRYVVSSASAWTHSLENGYHIHTFSGTTLTEHAVEKFKQLVWRPRPPTLLSKEEQKKVRRNLREYSREFDEEDKYAVDIANTAIVEMRKRLLNEWTAWLKKEKEMVEEEREVLGLPKYEEEPAAKPTPGAEDDTIVEEIVEEIIEESEEIVA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.; RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.2. The ideal protein would be one that plays a critical role in formation of cytoplasmic translation initiation complex through RNA binding, translation initiation factor activity within eukaryotic 48S preinitiation complex, eukaryotic 43S preinitiation complex, eukaryotic translation initiation factor 3 complex. The designed protein sequence is ",
    "response#1": "DGSRSIITYKIEDGVKYKITQKVKEVKVLEKVHKSVAERKNWHKYGSEKGSPAGPSAVTARLGEEVELRLSRNWKQAEEERIQKEKASLTKTGLQCRLCGNDHMTMNCPFK",
    "response#2": "DGSRSIITYKIEDGVKYKITQKVKEVKVLEKVHKSVAERKNWHKYGSEKGSPAGPSAVTARLGEEVELRLSRNWKQAEEERIQKEKASLTKTGLQCRLCGNDHMTMNCPFK",
    "response#3": "DGSRSIITYKIEDGVKYKITQKVKEVKVLEKVHKSVAERKNWHKYGSEKGSPAGPSAVTARLGEEVELRLSRNWKQAEEERIQKEKASLTKTGLQCRLCGNDHMTMNCPFK"
  },
  {
    "reference": "MTFLSTSAFSPLAFSLGLLLVVATAFPTPGPLGGDATSNRLPLTSADKMEELIKYILGKISALKKEMCDNYNKCEDSKEALAENNLNLPKLAEKDGCFQSGFNQETCLTRITTGLQEFQIYLKFLQDKYEGDEENAKSVYTSTNVLLQMLKRKGKNQDEVTIPVPTVEVGLQAKLQSQEEWLRHTTIHLTLRRLEDFLQFSLRAVRIM",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF; 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis; Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection ; Through activation of IL6ST-YAP- NOTCH pathway, induces inflammation-induced epithelial regeneration .; Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability; Also acts as a myokine ; Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand ; Plays a protective role during liver injury, being required for maintenance of tissue regeneration .2. Design the protein with a signal peptide incorporated.3. The designed protein should have interleukin-6 receptor binding, cytokine activity within interleukin-6 receptor complex, extracellular space to regulate positive regulation of receptor signaling pathway via STAT, glucose homeostasis, response to activity, vascular endothelial growth factor production, positive regulation of receptor signaling pathway via JAK-STAT. The designed protein sequence is ",
    "response#1": "AFSVGLERQVTVPNVPIRFTKIFYNQQNHYDGTTGKFLCNIPGLYYFSYHITVYLKDVKVSLYKNDKALLFTHDQFQDKNVDQASGSVLLYLEKGDQVWLQVYEGENHNGVYADNVNDSTFTGFLYVNGGQIHSHSQAYVYRSAFSVGLERQVTVPNVPIRFTKIFYNQQNHYDGTTGKFLCNIPGLYYFSYHITVYLKDVKVSLYKNDKALLFTHDQFQDKNVDQASGSVLLYLEKGDQVWLQVYEGENHNGVYADNVNDSTFTGFLLYHNIVY",
    "response#2": "MAMVPEPANEVMAYYSDENDLLFEADGPKQMKCCVQHLDLSSTGDGSIQLQICHQLHDKSFRQVVSVIVAMEKLKRVPCSQAFQDDDLRGIFSVIFEEEPEGTCDLHRCHRHGYCDKLSCFRKNPLVNAICEWRPSSTPSPTTKAVLFAKKINTTNGKSDFQVPCQYSQQLKSFSCQVEILEGDKVYHIVSLCVANSVGSKSSHNEAFHSLT",
    "response#3": "MAMVPEPANEVMAYYSDENDLLFEADGPKQMKCCVQHLDLSSTGDGSIQLQICHQLHDKSFRQVVSVIVAMEKLKRVPCSQAFQDDDLRGIFSVIFEEEPGEEGTGAGLDLSTSSLGSESAYVYRSAFSVGLERQVTVPNVPIRFTKIFYNQQNHYDGTTGKFLCNIPGLYYFSYHITVYLKDVKVSLYKNDKALLFTHDQFQDKNVDQASGSVLLYLEKGDQVWLQVYEGENHNGVYADNVNDSTFTGFLLYHP"
  },
  {
    "reference": "MELVDPNLEPWNQPGSKPTTACSTCYCKICCWHCQLCFLKKGLGISYGRKKRKHRRGTPQSSKDHQYPVPEQPLPIIRGNPTGPKESKKKVASKAETDQCA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should exhibit Basic and acidic residues compositional bias.2. A protein with actinin binding, protein domain specific binding, trans-activation response element binding functions is required to enable positive regulation of viral transcription.3. For general function, the protein need meet that Other factors such as HTATSF1/Tat-SF1, SUPT5H/SPT5, and HTATIP2 are also important for Tat's function; Through its interaction with host TBP, Tat may also modulate transcription initiation; Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication; Tat can reactivate a latently infected cell by penetrating in it and transactivating its LTR promoter; Besides its effect on RNA Pol II processivity, Tat induces chromatin remodeling of proviral genes by recruiting the histone acetyltransferases (HATs) CREBBP, EP300 and PCAF to the chromatin; The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive The designed protein sequence is ",
    "response#1": "MEPVDPNLEPWNHPGSQPXTXCNTCYCKKCCWHCQLCFLKKGLGISYGRKKRKHRRGTPQSRKDHQNPIPKQPIPQIRGNPTGPKESKKEVESKTETDXCA",
    "response#2": "MEPVDPNLEPWNHPGSQPTTPCSKCYCKKCCWHCQICFLKKGLGISYGRKKRKHRRGTPYSSKDHQNPVPEQPLPTTRGNPTGPKESKKEVASKAETDQWA",
    "response#3": "MEPVDPRLEPWKHPGSQPKTACNTCYCKKCCFHCQVCFTKKALGISYGRKKRRQRRRAPQDHQNSXSEPVSRPLSNPTGPEESKKKVERETETDPSD"
  },
  {
    "reference": "MSTAALITLVRSGGNQVRRRVLLSSRLLQDDRRVTPTCHSSTSEPRCSRFDPDGSGSPATWDNFGIWDNRIDEPILLPPSIKYGKPIPKISLENVGCASQIGKRKENEDRFDFAQLTDEVLYFAVYDGHGGPAAADFCHTHMEKCIMDLLPKEKNLETLLTLAFLEIDKAFSSHARLSADATLLTSGTTATVALLRDGIELVVASVGDSRAILCRKGKPMKLTIDHTPERKDEKERIKKCGGFVAWNSLGQPHVNGRLAMTRSIGDLDLKTSGVIAEPETKRIKLHHADDSFLVLTTDGINFMVNSQEICDFVNQCHDPNEAAHAVTEQAIQYGTEDNSTAVVVPFGAWGKYKNSEINFSFSRSFASSGRWA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must have at least one PPM-type phosphatase domain.2. The protein must have high specificity towards myosin phosphatase activity.3. The protein must bind to Mn(2+), Mg(2+) in order to perform its enzymatic function.4. The protein should have a fast turnover rate for H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +   phosphate.5. The protein should be able to bind Mg(2+) ligand in a variety of conditions6. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "DQEWLTVCPEEFLTGHYWALFDGHGGPAAAILAANTLHSCLRRQLEAVVEGMIAPQPPMHLSGRCVCPSDPQFVEEKGIQAEDLVIGALENAFQECDDVIGRELEASGQVGGCTALVAVFLQGKLYVANAGDSRAILVRRHEIRQLSSEFTPETERQRIQQLAFTYPELLAGEFTRLEFPRRLKGDDLGQKVLFRDHHMRGWSYKRVEKSDLKYPLIHGQGRQARLLGTLAVSRGLGDHQLRVLDTDIQLKPFLLSIPQVTVLDVHQLAVQEEDVVVMATDGLWDVLSNEQVALLVRSFLTGNQKDDPHRFSELAKMLIHNTQGKDNGATGEGQVSYDDVSVFVIPY",
    "response#2": "SPARGRTLPWNAGYAEVINAEKSEFNEDQAACGKLCIRRCEFGIEEDQEWLTVCPEEFLTGHYWALFDGHGGPAAAILAANTLHSCLRRQLEAVVEGMIAPQPPMHLSGRCVCPSDPQFVEEKGIQAEDLVIGALENAFQECDDVIGRELEASGQVGGCTALVAVFLQGKLYVANAGDSRAILVRRHEIRQLSSEFTPETERQRIQQLAFTYPELLAGEFTRLEFPRRLKGDDLGQKVLFRDHHMRGWSYKRVEKSDLKYPLIHGQGRQARLLGTLAVSRGLGDHQLRVLDTDIQLKPFLLSIPQVTVLDVHQLAVQEEDVVVMATDGLWDVLSNEQVALLVRSFLTGNQKDDPHRFSELAKMLIHNTQGKDNGATGEGQVSYDDVSVFVHTL",
    "response#3": "RPHAAGEFSMAAAQANLAMEDQAQVLASPAATLVGVYDGHGGADASRFLRSRLFPHVQRFEKEQGGMSTEVIRRAFGAAEEEFLQQVRQAWRQRPKMAAVGSCCLLGAISGDTLYVANLGDSRAVLGRRVVGGGVAVAERLTDEHNAASEEVRRELTALNPDDAQIVVHARGAWRVKGIIQVSRTIGDVYLKKQEYSMDPVFRNVGPPIPLKRPALSAEPSIQVRKLKPNDLFLIFASDGLWEHLSDDAAVQIVFKNPRTGIANRLVKAALKEATRKREVSFRDLKTIEKGVRRHFHDDISVIVRFGASAAQGWRARMEDAHCTWLSLPGLPPGWALFAVLDGHGGARAARFGARHLPGHVLQELGPEPSEPEGVREALRRAFLSADERLRSLWPRVETGGCTAVVLLVSPRFLYLAHCGDSRAVLSRAGAVAFSTEDHRPLRPRERERIHAAGGTIRRRRVEGSLAVSRALGDFTYKEAPGRPPELQLVSAEPEVAALARQAEDEFMLLASDGVWDTVSGAALAGLVASRLRLGLAPELLCAQLLDTCLCKGSLDNMTCILVCL"
  },
  {
    "reference": "MLVEAPTALEFDTIDDVLHDIAAGKIVIVTDDADRENEGDLVMAAEKATPEAVNFMTKHGRGLICVPVSNERAEQLGLQRMVAQNREMYRTDFTVSVDAGRGVTTGISAHDRSATIRTIADLQSRPEDLVQPGHIFPLRAKDGGVLRRAGHTEAAVDLARMAGLQPAGVLCEILHDDGTMARLPELIEFKRRHGLRICTIQSLIAQRRLKEKLIAREQVVKLPTDFGDFDLHLYRSTLDGSHHLALVKGTISSTEPVLVRVHSECLTGDVFGSRRCDCGNQLHAALQQISEAGDGVLVYMRQEGRGIGLAAKIHAYKLQEEGLDTVEANAKLGFGMDLRDYGLGAQILFDLGVRKFRFLTNNPKKVVGLEGYGIQMVEQVPIRSEANPHNAKYLETKRVKMGHLL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein contains novel GTP cyclohydrolase II domains that confer a unique function or activity.2. The protein must have high specificity towards 3,4-dihydroxy-2-butanone-4-phosphate synthase activity. The designed protein sequence is ",
    "response#1": "ALETKLPSRFGIFRLLVYEDQMESYHHLALVKGEVRGRKDVLVRVHSQCLTGDILGSLRCDCGQQLGKALQMIEREGRGVFLYMRQEGRGIGLYNKLRAYKLQDSGLDTIEANLALGFGADERDYGIGAQILKDLGLSSIRLITNNPEKITGLEGHGLRITRRIAEASLPTRFGQGRVIAYGVKHEDQQPIAIVMGEPMGVAEPLVRLHSSCFTGDLLASLRCDCGDQLQMALDMIGREGSGVLVYLPQEGRGIGLVEKIKAYNLQDQGLDTVEANLALGHHTDSRDYGVGIQVLKDLGLKRVRLLTNNPKKTDAFIYGGFDLEVVDQVL",
    "response#2": "ALETKLPSRFGIFRLLVYEDQMESYHHLALVKGEVRGRKDVLVRVHSQCLTGDILGSLRCDCGQQLGKALQMIEREGRGVFLYMRQEGRGIGLYNKLRAYKLQDSGLDTIEANLALGFGADERDYGIGAQILKDLGLSSIRLITNNPEKITGLEGHGLRITRRIS",
    "response#3": "ALETKLPSRFGIFRLLVYEDQMESYHHLALVKGEVRGRKDVLVRVHSQCLTGDILGSLRCDCGQQLGKALQMIEREGRGVFLYMRQEGRGIGLYNKLRAYKLQDSGLDTIEANLALGFGADERDYGIGAQILKDLGLSSIRLITNNPEKITGLEGHGLRITRRIS"
  },
  {
    "reference": "MPDSKYIFVTGGVSSSLGKGIIAASLAKLLQAQDFKVTIQKFDPYINVDPGTLNPYEHGECYVTDDGAETDLDLGHYERFLNIKTSQANNVTTGRVYQSVIEKERKGVFLGKTVQVVPHITNEIKERMQQLARTGEFDIVITEIGGTVGDIESLPYIESVRQLIYELGEENTMVIHLTLIPFLSAAKELKTKPTQHSVKTLMESGIKADVLVCRTEYELSNELKKKLALFCNVRVEAVIQSIDVETIYDVPIKMMEEGLDKVVLNKLKLKSKPPKLDHWKSFLYKYKNPKRKISIGLVGKYVELQDSYKSILEALIHAGAVNEAEVKVVSIHSEHLDQANSKEQLKSLDGLIVAPGFGGRGIEGKIDAITYVRKNKIPFFGICLGMQMAVIEYSRNILNYRNANSTEINENTDYPVIDLMESQKGIINKGGTMRLGTWDCKLIEGSKLVKVYGQEVIEERHRHRYEFNNEYLSEVLESGSFKVAGINPQTNLVEIVEDQEHPWFIGVQFHPEYKSTVSNPHPLFVDFIKASIDYLSRK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein contains novel CTP synthase N-terminal domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "LPDAYLSVTEALRAGGFAHSTKVNIHWVASDDCETPEGALEALGTMHAICVPGGFGIRGIEGKLGALRFARENGIPTLGLCLGLQCMVIEYARNVVGLEGASSTEFDADTSVPVIATMAEQVNILDGGDLGGTMRLGLYEARLADDSLAAELYGETLVSERHRHRYEVNNRYRDEIAAAGLVFSGTSPDGELVEYAELPTSEHPFYIATQAHPELRSRPGKPHPLFAGVRIAVVGKYVALQDAYMSVSEALRHGGIANDAAIEIKWINAEDVEEEHADLSVYLGDVDGILVPGGFGDRGVGGKLKAIRYARENKVPFFGLCLGMQSAVIEFARNVCGLADAHSTEFNPDTPHPVIDLMPEQMAVEDKGGTMRLGVYPCKVTENTLTYTAYEDEIIYERHRHRFEFNNAYRDQLSAAGLIISGTLPNGRLVEIVEVKDHPWFVGTQFHPEFKSRPTAPHPLFRDFVRIAVVGKYVALQDAYMSVSEALRHGGIANDAAIEIKWINAEDVEEEHADLSVYLGDVDGILVPGGFGDRGVGGKLKAIRYARENKVPFFGLCLGMQSAVIEFARNVCGLADAHSTEFNPDTPHPVIDLMPEQMAVEDKGGTMRLGVYPCKVTENTLTYTAYEDEIIYERHRHRFEFNNAYRDQLSAAGLIISGTLPNGRLVEIVEVKDHPWFVGTQFHPEFKSRPTAPHPLFRDFIFVTGGVVSSLGKGIAAASLAAILEARGLRVTMMKLDPYINVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERYVRTRLGRKNSITTGRIYENVIRKERRGDYLGGTVQVIPHITDEIKRGIFAATDGFDVGLVEIGGTVGDIESLPFLEAIRQIRAERGPDKALFMHLTLVPFIAAAGELKTKPTQHSVKELRSIGIQPDILICRSEQPLPDSERRKIALFTSVSERAVISAVDLQNIYDMPARFQEQGLDELIVERLH",
    "response#2": "LHDSYKSITEAFTHAGAATETEVKVRWVYSGDLTEETVAEKLAGVSGVLIAPGFGDRGILGKIIAANFARRNNIPTLGICLGMQIMAIEFARNVLGYEKADSAEMDLTTPHPVISLMEDQKDVSDKGGTMRLGNWKCTLAEGSKIRNVYGSEDILERHRHRYEFNNEYYDEFVNNGLIPVGKNPETELVEILEHKEHPYYIGVQFHPEYKSTVASPHPLFISFIDAYKSIYESLDHAGMANSAQVRIARIRSEELEVEGPERMLAGYDGILVPGGFGERGVEGKIAAIRYAREKGVPFFGICLGMQCAVIEFARNVCGLAAAHSTEFDKDTPHAVICLLDEQRTITDKGGTMRLGAQPARLDPESRSAKAYRAIEISERHRHRYEVNNRYRQQFVAHGLRIAGTSPDGQLVEIIELAQHRWFVAVQYHPEFKSKPTAAHPLFAGFIY",
    "response#3": "LHDSYKSITEAFTHAGAATETEVKVRWVYSGDLTEETVAEKLAGVSGVLIAPGFGDRGILGKIIAANFARRNNIPTLGICLGMQIMAIEFARNVLGYEKADSAEMDLTTPHPVISLMEDQKDVSDKGGTMRLGNWKCTLAEGSKIRNVYGSEDILERHRHRYEFNNEYYDEFVNNGLIPVGKNPETELVEILEHKEHPYYIGVQFHPEYKSTVASPHPLFISFIDAYKSIYESLDHAGMANSAQVRIARIRSEELEVEGPERMLAGYDGILVPGGFGERGVEGKIAAIRYAREKGVPFFGICLGMQCAVIEFARNVCGLAAAHSTEFDKDTPHAVICLLDEQRTITDKGGTMRLGAQPARLDPESRSAKAYRAIEISERHRHRYEVNNRYRQQFVAHGLRIAGTSPDGQLVEIIELAQHRWFVAVQYHPEFKSKPTAAHPLFAGFIY"
  },
  {
    "reference": "MNEENPSSSIILDDQQQQDSFYSEIKELEKSIQSNKLSERKNTLTRINSIGKHELSTQENDATTTTTTTTVDLEEKLKVLIYLYFTSYSIGPDSQWIFSLVQSLKQLFKDISSATNTSLVTDELKINIVKFTLREIGRLAKLLPTNKRSKYVPQNTLMSFSLVLLNYFIDQIQSNSDLTTLLFAAQELLYRELTLQHMKNNQPIFNQILNKNKILTFYQNIVTKDSSNKSFFIVYFLLRNFTDQSNKQKEQDNTFKINDLLNIYNKTVIGSSQQKIEEHKFFKRLFNQLTNEDLQSIILPPLSRHIKRDQDQVFKILIFILENLSSDFNVIDLSSLLKSMLLPMLLPVIQSTISIEENRKLLKKTFTLIIERSKDTKLISSMITDDLLKTLSVAGNPSQKLIIISIISSIISTKNFIERLSLTTEKLQLSKQILQSISIYLEKELNKDNRNKGFKLLGKVMKMVEELPEQTIKIITNSLKNDDDIIKGQVILSLSKSLGPEANGTNKKVIQIINGFTETINTILKNVKNAKTCDPSTTTASLHYMLSLITTTGVPKNDIFTKYSTDKTTISNLYASTSFLHTDGFIQRTSKKDHAIDLLLTLFLRVKSFPSIKLNDKSPLYSSVLNCLLHSQWSVSKHSAIKIRSILSRNDSVDIDYPLLSNQLLIEFSTILFDDSLIITPQIINSNVESTTTTTTTISNKKNYLIAFRSILSKNIKSELYPMLSLICYHPFINYNWKRVSSLIQNDVNTTLSSNAIEISKYIFEKGL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be involved in cellular response to hydrogen peroxide, regulation of translational elongation by localizing to cytosol and exhibiting ribosome binding.2. The protein must exhibit the following characteristics: Forms a complex with eif2ak4/gcn2 on translating ribosomes; during this process, gcn1 seems to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of eif2ak4/gcn2, and hence stimulating eif2ak4/gcn2 kinase activity; Acts as a positive activator of the gcn2 protein kinase activity in response to amino acid starvation. The designed protein sequence is ",
    "response#1": "DQRVLFVDALSSLSLKHIDASMLLNELLPLFTKAKESDFNSLASLIVKTLKFLLMNGRNPGDKIYDFLSKSLQRPVAHESMFWLTSLATMAWDLPASDDVQIEFINFFLNNLSILTEKALMSVSGATQNGTYLAPIIYLSFGVNKLSVWNSERISHTLELQDILVKLSTPKNNDVFIFSSKITNKLNDDQSKLWYFQGLCDFAKVSDNLLFSNFVERWFQSVIGVFSFASRENSNRALKILKSAILYRPHLRMSICSQLWNYHADFEKSKSVGKFDSAKYDEISSLFQSLILSSMSADTSNFSNQELVDFDKYLVELLFLSFAFKDKFDWIRFCQVSKRDPATLVSF",
    "response#2": "DQRVLFVDALSSLSLKHIDASMLLNELLPLFTKAKESDFNSLASLIVKTLKFLLMNGRNPGDKIYDFLSKSLQRPVAHESMFWLTSLATMAWDLPASDDVQIEFINFFLNNLSILTEKALMSVSGATQNGTYLAPIIYLSFGVNKLSVWNSERISHTLELQDILVKLSTPKNNDVFIFSSKITNKLNDDQSKLWYFQGLCDFAKVSDNLLFSNFVERWFQSVIGVFSFASRENSNRALKILKSAILYRPHLRMSICSQLWNYHADFEKSKSVGKFDSAKYDEISSLFQSLILSSMSADTSNFSNQELVDFDKYLVELLFLSFAFKDKFDWIRFCQVSKRDPATLVSPLVPAIKEDAGGMIQRLMQQLLESDKYAERKGAAYGLAGLVKGLGILSLKQQEMMAALTDAIQDKKNFRRREGALFAFEMLCTMLGKLFEPYVVHVLPHLLLCFGDGNQYVREAADDCAKAVMSNLSAHGVKLVLPSLLAALEEESWRTKAGSVELLGAMAYCAPKQLSSCLPNIVPKLTEVLTDSHVKVQKAGQQALRQIGSVIRNPEILAIAPVLLDALTDPSRKTQKCLQPLVPAIKEDAGGMIQRLMQQLLESDKYAERKGAAYGLAGLVKGLGILSLKQQEMMAALTDAIQDKKNFRRREGALFAFEMLCTMLGKLFEPYVVHVLPHLLLCFGDGNQYVREAADDCAKAVMSNLSAHGVKLVLPSLLAALEEESWRTKAGSVELLGAMAYCAPKQLSSCLPNIVPKLTEVLTDSHVKVQKAGQQALRQIGSVIRNPEILAIAPVLLDALTDPSRKTQKCLQ",
    "response#3": "NEILPQLIQLLDNSNNKDLAGYALDGLRQVILVRSSIVLPVLIPKLLSRPISTSNVTALSSLAADAGEGLYVHLSTIIPSLIESFTNPNTISNAKEIKEAAVSICKSIDEQGWDTLIGLLIEQTEIRLPNIRLGACELIGEFYNGNTMVTEYPEELLLSLLSLFNDPDALVQQAANNALGFITKSLKKDNLTYLPVFQKGIQLLVNETYEEVSTIPGFCLPS"
  },
  {
    "reference": "MKKPIEFYLSGDRVYSTREKAINQLYNNRGYGELKGDKLFLSLIEAAYLTEKGWIKVVDKDKELKFDDLMKLGKSRDEDFDIKYIVYKDLRDRGYIVKSALKFGSHYRVYRKDAEHSDWLIWVLRENQRLSPNDITARARVAHGVRKNMVLAIVDEDGDVVYYKVEWIKF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The active site of the protein must be engineered to enable effective catalysis of pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-   half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with   a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus..2. For general function, the protein need meet that Endonuclease that removes tRNA introns; Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron; The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini The designed protein sequence is ",
    "response#1": "SSLENVRIIIDDLVQNFPDLIKEKPADELINECFQKALDYKPTFVKNMTANTIKKDPTYYGIAMHYSSILENLEIVSHNEHFQNIKSHIQTEFHVTLGHIASSKQDKAGRVKWKKLVKTLGKGDPNKPKSALKFFADVKLLQIVINTDKLACIKVEILKIYDTNDVLQSEIEPINKQLHITIGCIPPATAVESNITLEEF",
    "response#2": "SSLENVRIIIDDLVQNFPDLIKEKPADELINECFQKALDYKPTFVKNMTANTIKKDPTYYGIAMHYSSILENLEIVSHNEHFQNIKSHIQTEFHVTLGHIASSKQDKAGRVKWKKLVKTLGKGDPNKPKSALKFFADVKLLQIVINTDKLACIKVEILKIYDTNDVLQSEIEPINKQLHITIGCIPPATAVESNITLEER",
    "response#3": "SSLENVRIIIDDLVQNFPDLIKEKPADELINECFQKALDYKPTFVKNMTANTIKKDPTYYGIAMHYSSILENLEIVSHNEHFQNIKSHIQTEFHVTLGHIASSKQDKAGRVKWKKLVKTLGKGDPNKPKSALKFFADVKLLQIVINTDKLACIKVEILKIYDTNDVLQSEIEPINKQLHITIGCIPPATAVESNITLEEV"
  },
  {
    "reference": "MKTVTPEEMRDLERRSEEEGVSTDALMERAGLLAARVAWNMLGEGAYGSQIIILVGSGNNGSDGLVTARHLQSWGARTTAFLLAPRAPDDPKLQPASEVGVTIVEAADSSHLTDIQSELSEAALVVDAVLGTGRSRPLEGIYRDVLAAANAERERRPELVILALDIPSGVDAETGSADADALVADATATFGFPKTGLFRFPGADLMGQITVADIGIPEGLSANIRQEVVTQAWAQAYLPTRPLSAHKGTFGRVLALVGSRNYVGAAYLACMGAARSGSGYVTLAATPSLQSLVASKLTEPTYLLLPCDTDGDPTAEGALAVRDCLPNCDVLLAGCGLGQHDGTNALLEQLLLSSVPLSVPIVLDADALNTLSGIPSWYSRLQSPTVVTPHPGEMSRLMGTSITDVEGDRVATAQKAAAEWGVTVLLKGAYTVVASPEGDVRIIPFANPGLATAGTGDVLAGVIASLIAQGVLPYDAASLGAYLHAAAGEMVTNDIGNTGLVASDLLPMIPRAIKSVREGTFSGGIREIS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. The desired protein should be able to perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate3. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.4. The protein contains novel YjeF N-terminal domains that confer a unique function or activity.5. The protein must exhibit ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding, metal ion binding, NADPHX epimerase activity, NADHX epimerase activity.6. The designed protein should be able to compete with endogenous proteins or inhibitors for (6S)-NADPHX, K(+), AMP binding. The designed protein sequence is ",
    "response#1": "MGRLDRLAESQFGIPSIVLMENAGLRVFESIKRYFHNFLAGRRVAVFCGKGNNGGDGIVVARHLLNQGSEVKVFLLGSPQDLKGDPQINLKIYQKMGGRLYPLTEENHLQRVDISLLYADLVVDAIFGTGFKGAALGLAAEVIQRINGSDKPVVAVDLPSGLEADTGKVWGPCVRARWTVTFALPKLGLVVEPGASYAGALEIADIGIPYKLVEAAAEAGCRAGIIGGNTGMNGALQLAGASALYSGCGKVSMIPLDQSFKADMSMPELMTKSLKNVPKNINIFSALAVGVGFDTTDDSQQILESIIDNLTQLAIFDADALNIIVTNQQIREKFINLENKIITPHPAEAARLLDCTTKEIQEDRFTAIRTLAKKYNATVVLKGAGSLICKDNKIYINTTGNQGMAVAGQGDVLSGIIGAFLAQGLNTLSASRLAVYVHGLAGDNVAKKLRFTADGTCLPKREKTAHKGLFGHVLVAGGERGFSGAVQMAASAALRVGAGLVSLATRSEHAALLNLVRPELMVHGIDKRVDLAPLLKKSSVAAVGPGLGQTVWAQNLLAELFDTAMPLVVDADALNLLAKEPFKRDNWVLTPHPGEAARLLGITIKEIESDRFSSVKALQKKYGGVVVLKGAGTLIFDGSEMVINTTGNPGMASGGMGDVLSGVIAALLAQGLSLSQSARLGVWLHGKAGDIAAKEGERGLLASDLLRPLRVLVND",
    "response#2": "MAKYDGYAIKTWGIPSTVLMENAGRNTYRLMKEQYLAGAAAIAVVCGRGNNGGDGFVISRYALRDGFRTTVFLLGKRSELKGDAALNMELYASIGGEVVECTKDIDALRKGIQGADVLVDAIFGTGLSKATGGNEKLAIEAMNGSGKPVIAVDIPSGIDGKTGLPLGAAIRATHTFTYGYPKLGQILYPGAYHAGRLTVIDISIPASIEANVNKYSRGSVLVVAGSARYPGAAMMAAKSAARAGAGYVAVAAPDACANLIRMALPSIPVIAIPSDSRGSFGAAARQVVCEIAKKYDCVLCGPGMTTASGCMQVVSGLLELDVPLILDADALNCLARLSIDGIDKTPELYRREAPLILTPHHRELSRLVGDVPVDDLGTAIDAAQRIEWAVGSDNLVVVAKGATTAIVGVEKVLIPISGPASLATAGSGDVLAGIMAGTVATAHGDVDRWELLCAYAVAVHSYTGFAAAAEYGEKSVIATDLIDLPSSHKGTYGHVLALGGSPGFTGAITLTARAALRAGAGLVTAAVPRGCQPVVAAGAPEIMTWGLPETAGGSLGRSALDPLLERLENFSVLAIGPGLGRDPETVELVKSLLPRASIPLVVDADALNALATDTGILSQPHGPMVLTPHPGEMGRLIGCPAAKIQEDRLNIARRYAQEWRCVLLLKGARTVVACPDGRAYINPTGNPGMATGGSGDVLTGIIAGLIAQGMSLDKAAAIGAYLHGAAGDLAAEELGQEALIAGDLALGGSPGFTGAITLTARAALRAGAGLVTAAVPRGCQPVVAAGAPEIMTWGLPETAGGSLGRSALDPLLERLENFSVLAIGPGLGRDPETVELVKSLLPRASIPLVVDADALNALATDTGILSQPHGPMVLTPHPGEMGRLIGCPAAKIQEDRLNIARRYAQEWRCVLLLKGARTVVACPDGRAYINPTGNPGMATGGSGDVLTGIIAGLIAQGMSLDKAAAIGAYLHGAAGDLAAEELGQEALIAGDIL",
    "response#3": "MYQADRLAMAAGVPGERLMEAAGWAVAREVRRRFPKGRVAILCGPGNNGGDGFVAARLLERDGWAVRLALLGRPEALKGDAAVMASRWRGAVETLSPGCLDRCDVVVDALFGAGLARGLDGIAKAVIEETLRRRLPVIAVDVPSGVHGDSGQVLGLAPDCVATITFFRKKPGHCLVPGRAKCGEVVVADIGIPASVLEAGRVKDLLKPRTRFMHKGHFGHVLLIAGSYGKMGAAVLASRACMKTGVGLLTVQVPHKTCHVLHCSVPEAMVNIDRSDLMFTEFPDLSPFKAVGVGPAIGCRSNSVLALTDLLDQIGSRPMVLDADALNILSNNPALLEKLPENAVLTPHPGELKRLVGPWGDDFERLQKVVAFCRKYQVVVVLKGAFTTVISSNGECHFNTTGNPGMASAGSGDVLTGIILSLLGQGLSSMDAAVAGVHIHGAAGDLACAAEGEAALTASDIVSHLGKAIVARALPQRPGNAHKNQCGRVLVVAGSTGYTGAAALCARAAQKSGAGLVSLCIPRSLDNILEIKLTEEITCPLADGNKGYLTEKNADAILALAGEYQVLAIGPGLGRNASTAAMVRKVVARSPVPVVLDADGINAYEKAGHLLKKAKSPVIITPHDGELKRLTGEKEIGWGPARIRALRNIQKELGITIVLKGGPTLVVSSTCMVNVNTTGNPGMATAGAGDVLTGLISGLYAQTHNLEQAVLTGVYLHGLAGDYGAMACTEYCLTAGDIITFFPQGMIRDREENSHKGTFGTLAILAGSADMPGAAYLCATGALHAGVGLVKLASDASALGVLKNRLSEPVFLSGGAEAAAQTSKTALLVGCGLGRGFDASLGALLRGSDVPTVIDADGINFLASHIDVLKEMQAPAALTPHPMELSRLTGCGVEEINRNRVRTARAFAAEHGCFLLLKGSRTVIAAPDGRVRITVSGCSALAKGGSGDVLAGVVGALAAQGYDLFDALTVGAFLHGRAGERLAARFGAHGALPSQLPEEIGR"
  },
  {
    "reference": "MNSEIKSLPLLPLRGILVFPYMVIHLDVGREKSIQAIEEAMVQDRMIFLATQREAQTDEPTVDDIYNIGTVAEVKQLLKLPGGTIRVLVEGIARAKIEKYEHQDPYFRVEVQQYSEEFEKGAEVEALMRSLVYQFEQYVKLSKRIPPETVVSVVNLEEPGRLADIIASHLALKIEDKQNVLESVEIVDRLEKLCGIVAKELEIVELERKINIRVRKQMEKTQKEYYLREQMKAIQKELGEKDERVAECEEFREKISKAKFPKEAEEKALKEVERLEKMPPMAAEAAVVRNYLDWMLSLPWSKSTKDRIDINAAEEVLEADHYGLKDPKERITEYLAIRKLAKKMKGPILCLVGPPGVGKTSLGRSVARALDRKFVRISLGGVRDEAEIRGHRRTYVGAMPGRVIQGMRTAGSKNPVFLLDEIDKMASDFRGDPSSALLEVLDPEQNSTFSDHYIETPFDLSNVMFITTANNMYSIPRPLLDRMEVIQISGYTEEEKLQIAKRHLMPKQIKDHGLTEEMIQISENTILKVIREYTRESGVRNLERKIASICRKTAKKIVAGQAEKVKVTTQNLEQFLGIPRYRYGVAEQNDEVGTVTGMAWTEVGGDTLVIEVTTYKGTGRMTLTGKLGDVMKESAQAGYSFIRSRAQELGIDQEMFEKWDLHIHIPEGAIPKDGPSAGITMATAMASVLTGRKVRHDIAMTGEITLRGRVLPVGGIKEKVMAAHRAGIKLIILPNDNKKDLEDIPVNIKKQLEFKLVDHIDQVLAIAL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein can be targeted to cytoplasm and perform sequence-specific DNA binding. The designed protein sequence is ",
    "response#1": "KERLSAFLCANADGTHKLKSIIIGKSKLPKSVKEDTSTLPVIYKPSKDVWFTRELFSEWFFQNFVPEVRHFQLNVLRFHDEDVRALLLLDSCPAHPSSESLTSEDGRIKCMFFPHNTSTLIQPMNQGVILSCKRLYRWKQLEESLVIFEESDDEQEKGDKGVSKIKIYNIKSAIFNWAKSWEEVKQITIANAV",
    "response#2": "KERLSAFLCANADGTHKLKSIIIGKSKLPKSVKEDTSTLPVIYKPSKDVWFTRELFSEWFFQNFVPEVRHFQLNVLRFHDEDVRALLLLDSCPAHPSSESLTSEDGRIKCMFFPHNTSTLIQPMNQGVILSCKRLYRWKQLEESLVIFEESDDEQEKGDKGVSKIKIYNIKSAIFNWAKSWEEVKQITIANAS",
    "response#3": "KERLSAFLCANADGTHKLKSIIIGKSKLPKSVKEDTSTLPVIYKPSKDVWFTRELFSEWFFQNFVPEVRHFQLNVLRFHDEDVRALLLLDSCPAHPSSESLTSEDGRIKCMFFPHNTSTLIQPMNQGVILSCKRLYRWKQLEESLVIFEESDDEQEKGDKGVSKIKIYNIKSAIFNWAKSWEEVKQITIANAV"
  },
  {
    "reference": "MNQYIDFNSTIYPFPPKPAFLSKDEKKHYREKIKRLLKQQDAVMVAHYYTDPEIQALAEETGGCVADSLEMARFGNNHPASTLLVAGVRFMGETAKILNPEKRVLMPTLEAECSLDLGCPEKEFTQFCDDHPDRTVVVYANTSAAVKARADWVVTSSIAVELIDYLDSQGKKIIWAPDRHLGNYVRKQTGADILCWQGACIVHDEFKTQALIRVKGLHPDAAVLVHPESPQAVIDLADAVGSTSQLIKSAQSLPHQKLIVATDKGIFYKMQQACPEKQLFAAPTAGEGASCRSCAHCPWMAMNGLQAIVQGLEQGGGQHEILVEKELREKALVPLNRMLDFAAQLK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have a high specificity for iminosuccinate, [4Fe-4S] cluster binding, with minimal off-target effects.2. The desired protein should be able to perform dihydroxyacetone phosphate + iminosuccinate = H(+) + 2 H2O +   phosphate + quinolinate3. A protein that can modulate the overall flux of metabolites through the NAD(+) biosynthesis pathway, enhancing or suppressing its activity as needed.4. The designed protein should have metal ion binding, 4 iron, 4 sulfur cluster binding, quinolinate synthetase A activity within cytoplasm to regulate NAD biosynthetic process.5. The protein requires [4Fe-4S] cluster to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "MLKGRFISISGNEGKTTVAINLACSLANSGFKV",
    "response#2": "MNNEVKTKEVV",
    "response#3": "MDLKDKIALVTGASRGIGRSIAIELGKNGAKVIVNYANSKEAAEEVVKEIEVLGTKAVSIKADVSKISDVEKLFNKTIETFGGLDILVNNAGITRDTLLMRMSEEEWDNVIDTNLKGVFNCIKSASKVMKQQKSGRIINIAXIVGFTGNPGQANYSAAKGGIIGLTKSMAKELGARNINVNAVAPGFIVSDMTDALSDELKEMLLSQIPMGRFGKPEEVAKAVLFLASSDSSYMTGQVLHVDGGML"
  },
  {
    "reference": "MAIEGPVAIILAAGQGKRMNSDRAKVLHEVCGRPMISYVVDAARGAGAKTIVVVVGYAADQLYEALDGEPDIRFAVQEEQLGTGHAVKVCRPLLEGYEGPILVLVGDEPLLRPEPLADLLDRQKRERAACLLGTAEVDDPTGFGRILRDSAGRFLRIVEERDGTPEERAIREVNPSCYVFELPALWEALEQIGTSNAQGEYYLTDAPEQLMRMDRKVLALNVLSADDILGVNTRQHLAQAGEIMQARIQDHWMTEGVSIVDPRNTYIDGRAQIGRDTVVRPFSVLDGAVKVGDRCKIGPFAHLRPGAELADDVEVGAFVEVSRSTLDAGVVVRHLAYLGDARVGANVNVGCGAITANFDGERKHATTIGADAHIGSGAVLIAPVAVGEGAVVGAGAVVTKNNDVPKGATVVGVPARPIATKSE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein requires Mg(2+) to activate a critical catalytic site.2. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.3. A protein that can perform magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity, glucosamine-1-phosphate N-acetyltransferase activity in cytoplasm to support cell morphogenesis, peptidoglycan biosynthetic process, regulation of cell shape, UDP-N-acetylglucosamine biosynthetic process.4. The designed protein have MobA-like NTP transferase domains with specific sequence motifs that are crucial for its activity.5. The protein design should take into account the physiological conditions of the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway.6. The protein should have a high affinity for Mg(2+), acetyl-CoA. The designed protein sequence is ",
    "response#1": "VVILAAGMGKRMQSALPKVLHPLAGKPLLAHVLDTARSLATNRVCVIYGHGGAAVLELLGKQPQPVATAQQEQQLGTGHAVMQALPELDDNAPTLILYGDVPLTTAASLQALIDAAGTDKLGILTVVQDPNHPQGYAQLVTVVVLAAGEGTRMKSATPKVLHAICGRSLVGHVVAASRELDPEHLVVVVGHAREQVTAHLAEIDASVRTAVQHEQNGTGHAVRMGLEELTSGGSGEAGAPGVELDGTVVVVCGDTPLLTGETLKALAETHAADGNAVTVLSAEVPDATGYGRIVRDADGTVARIVEHKDASDDERAIREINSGVYAFDAASLRSALGRLSTDNAQGELYLTDVLGIARGDGHRVGAHLAPDAALVAGVNDRVQLATLGAELNRRIVTRHQLA",
    "response#2": "MTEALILAAGEGTRMKSALPKVAHRILGVPLVRYVIDAAHTAGCEQVVVVTGHGAETVEALVGAETCVRQEEQLGTGHAVMCAEKALEGLTGALVVLSGDSPLLRPETIARLVDARETSRAVAVVLTTTLADPAGYGRIVRDELGKLVAIREHKDASEAERKVTFCNSGVIAFRSADLLRLLGRIGNANAKGEYYLTDAVEIARQAGLNVVAVEAPEAEVLGANNRVQLAEAEAGFQARLRQAAMVAGVTIVDPLSTWIDVEVTAEPDVVIHPGTQLHGRTHLAEGAEVGPHSTLTDTLVGADAVVANTVCVEAEIGPEATVGPYAYLRPGTRLARKAKAGAYVEMKNAVVGEGSKVPHLTYVGDAEIGEGSNIGASCVFVNYDGVQKHRSVVGDHVKVGSDSMIVAPVTIGDGAYTAAGSVIVKDVPPGAMAVARGRQRNIEGWVEKNRPGTAAAKLASAILASRKKKK",
    "response#3": "VVILAAGMGKRMQSALPKVLHPLAGKPLLAHVLDTARSLATNRVCVIYGHGGAAVLELLGKQPQPVATAQQEQQLGTGHAVMQALPELDDNAPTLILYGDVPLTTAASLQALIDAAGTDKLGILTVVQDPKNPNGYGRIVRNNNGSVVGIVEQKDATAEQKQIQEINTGILVASGADLKRWLGQLRNNNAQGEYYITDIIAMAYQDGIAIHTAQPENAWEVEGANNRLQLAGLERRYQLNQAKNLMLEGVAIIDPTTTWIDISVKIEADATIHPGSGLYGKTTVAKSAVIGPRTTLTDVSVGCCATVLESHCTAATIGDNANVGPFSFIRPGTVLAAGAKVGAYVEVKNSTIGEGSKVPHLSYVGDATIGSGSNIGAATIFVNYDGVDKHRTVVGDEVRIGSDTMLVAPVTIGDGAYTAAGSVITEDVPAGSIAVARSKQRHIQGWQRPVKVKK"
  },
  {
    "reference": "MRVKGIRRNYQHLWRGGTMLLGILMICSATEDLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEIVLGNVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDLKNVTYANNSSKEGDMTNCSFNIPTSRRGKVQKEYAFFYKLDVVSIDNDNTSYRLISCNASVITHACPKVSFEPIPIHYCAPAGFAILKCNDKKFNGMGSCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEDIIIRSQNISDNVKIIVVHLNESVQINCTRPNNNTKKSIQLGPGRAFYTTGDIVGDIRKAHCNISGTQWNRTLEQVKAELRPHFPNGTTIKFDSSSGGDLEVTTHSFNCRGEFFYCNTSGLFNETEFNGTITLPCRIKQIVNMWQEVGKAMYAAPIAGNITCNSNITGLLLTRDGGQNNNATEIFRPGGGNMKDNWRSELYKYKVVEIEPLGVAPTRAKRQVVRREKRAVGIGALFLGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLNAIWDNMTWMEWEKEIENYTDLIYTLIEQSQNQQEKNELELLELDKWASLWNWFDISTWLWYIRIFIMVVGGLIGFRIILAVLSIVNRVRQGYSPLSFQTHLPARRGPDRPEGIEEEGGDRDRDTSTRLVGGFLPLIWDDLRSLSLFSYHRLRDLLLIVTRIVELLGRRG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be involved in mitigation of host antiviral defense response, actin filament reorganization, virion attachment to host cell, viral protein processing by localizing to host cell endosome membrane, host cell plasma membrane and exhibiting structural molecule activity.2. The designed protein have Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains with specific sequence motifs that are crucial for its activity.3. Incorporating a YXXL motif; contains endocytosis signal motif is critical for the successful design of the protein.4. For general function, the protein need meet that During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; Transmembrane protein gp41: Acts as a class I viral fusion protein; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state5. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "MRVRGTQRNYPPWWIWGILGFWMLIICNVVGNLWVTVYYGVPVWKDAETTLFCASEAKAYKTEVHNVWATHACVPTDPNPQEIDLENVTEKFDMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLHCTNVTVKNSNTSITEQEMKNCSFNTTTGIRDKKQKAELDIVPINKNNASHNDSEYILINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCKDEQFNGTGLCRNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVMIRSENISDNAKTIIVQLTKPVSINCTRPNNNTRKSIRIGPGQAFYATGDIIGDIRQAHCNVSKSEWNAALEQVADKLGKHYNNNTIIFTKSSGGDLEITTHSFNCGGEFFYCNTTGLFNSTWPNNKTEKANEAEKANDTITLPCRIKQIINVWQRVGQAIYAPPIEGLIKCNSNITGLLLTRDGGDRNTSINETFRPGGGNMKDNWRSELYKYKVVRLEPLGVAPTKAKRRVVERQKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLKAIEAQQHLLRLTVWGIKQLRARVLAVERYLKDQQLLGIWGCSGKLICTIAVPWNSSWSNKTQNEIWDNMTWQDWEKEISNYTEIIYSLIEESQIQQEKNEQDLLALDKWASLWNWFDISKWLWYIKIFIIIVGGLIGLRIVFAVFSIINRVRQGYSPLSFQTRFPAQRGPDRPEGI",
    "response#2": "MRVKEIRKNYLWRWGTMLLGMLMICSAAEQLWVTIYYGVPVWKEATTTLFCASDAKAYSTEKHNVWATHACVPTDPNPQEVVLENVTENFNMWKNNMVEQMHEDIIGLWDQSLKPCVKLTPLCVTLNCTNYERNGKNTTRQDSTKVNNNTEIGNLTEEMRNCSFNITTEIRDKQRNVHALFYKLDIVPIGEKNNSDEYRLINCNTSVIRQACPKMSFDPIPIHYCTPAGYAILKCNDKNFNGIGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENITDNAKTIIVHLNESVEINCTRPSNNTRTSIPIGPGQVFYRTGDIIGDIRKAYCEINGTKWNDVLTQVAKKLRGHFNKTIVFQPPSGGDLEIMTHHFNCRGEFFYCNTSKLFNIACNKTEECKGYNNTITIPCRIKQIINMWQGAGQAMYAPPVRGIINCVSNITGILITRDGGNDSSTNGNEIFRPTGGNMRDNWRSELYKYKVVKLEPLGVAPTKAKRRVVEKEKRAXGLGALFLGFLGAAGSTMGAASVTLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTTVPWNTSWSNKSLDTIWNNMTWMQWEREIDNYTGLIYTLLEESQNQQEKNEQELLELDKWASLWNWFDITNWLWYIKIFIMIIGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTRLPTQRGPDRPE",
    "response#3": "MRVKGIRKNYQHLWRWGTMLLGMLMICSAAEQLWVTVYYGVPVWREATTTLFCASDAKAYATEVHNVWATHACVPTDPNPQEIDLVNVTENFDMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTKPNNNTETKNSTEGMIKGGEMKNCSFNITTGIKDKVQKDYALFYNLDITQIKDDSNSSYNSSYRLRSCNTSVITQTCPKVSFEPIPIHYCAPAGFAILKCKDEKFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAKEEVVIRSANFTDNTKIIIVQLNASVEINCTRPGNNTRKSINMGPGRAFYATGDIIGDIRQAHCNISRAKWNETLKQVVEKLEEQFKNKTISFNQSSGGDPETVMHTFNCGGEFFYCKTTELFNSTWTRNGTDKWSSTNTGNETIILPCRIRQIINMWQQVGKAMYAPPISGYINCTSNITGMLLTRDGGNNNETNGTEIFRPAGGDMRDNWRSELYKYKVVQIEPLGIAPTKARRRVVQREKRAVGIGALFLGFLGTAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKSQKEIWENMTWMQWDREISNYTNTIYSLLEESQIQQERNEKDLLALDSWKNLWSWFDISNWLWYIRIFIMIVGGLIGLRIIFTVLSIINRVRQGYSPLSLQTLLPTQRGPDRPEGTEEE"
  },
  {
    "reference": "MAETHSLGTKILIKIIRLYQIMISPFIGARCRFVPTCSCYGIEALKTHGLLKGGWLTLKRVLKCHPLNAGGFDPVPPKTNNNDEKK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Could be involved in insertion of integral membrane proteins into the membrane..2. The designed protein should have a specific function within the plasma membrane. The designed protein sequence is ",
    "response#1": "ECFHRR",
    "response#2": "GSSGN",
    "response#3": "MPNS"
  },
  {
    "reference": "MIKVAVNGAAGRMGGRLITAIKETEGLQLTGALEHAESPMLGQDAGLTAGCGPLDVLISSDLDEVLKATDVLIDFTFPEVSLRNLEACVRHDKSIVIGSTGFTPEQRARVDEMAQNIPVVLAPNMSVGVNACFKLLKEAAQILGDGFDVEIVELHHNKKKDSPSGTAVRMGEIVADALGRDYNKSAVYHREGMCGARTPEEIGMQTVRGGDIVGEHTVYFIGMGERIEITHRAMSRDMFARGAVRAASWLAGKAPGLYDMQDILGLK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.2. The ideal protein would be one that plays a critical role in diaminopimelate biosynthetic process through NADP binding within cytoplasm.3. The designed protein must have a Proton donor/acceptor that is structurally stable and resistant to denaturation.4. The designed protein should interact with enzymes and substrates within the L-lysine biosynthesis via DAP pathway, (S)-tetrahydrodipicolinate from L-aspartate: step 4/4 pathway to enhance or regulate its activity.5. The NAD(+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MRVAVSGAAGRMGRLVVKNAVAEGLKVVQAFDINEVGKDAGELAGVGKIGVPIEDDISKLDADVLIDFTTAEAAMKNAEVAAEKGVRVVMGTTGFTDEDRKRLAELAEKVPMIVSPNFAIGAVLMMHLAKLAGRHFDYAEIIELHHEKKADAPSGTALATARAMLSARGRPFEHPSTAKETLAGTRGGVLDGITIHSVRLPGLVAHQEVILGTLGQTLTIRHDTTSRESFMPGVLLAIKEVMNRQELVVGLDKL",
    "response#2": "MRVAVSGAAGRMGRLVVKNAVAEGLKVVQAFDINEVGKDAGELAGVGKIGVPIEDDISKLDADVLIDFTTAEAAMKNAEVAAEKGVRVVMGTTGFTDEDRKRLAELAEKVPMIVSPNFSVGAVLMMHLAKLAGRHFDYAEIIELHHEKKADAPSGTALATARAMLSARGRPFEHPSTAKETLAGTRGGVLDGITIHSVRLPGLVAHQEVILGTLGQTLTIRHDTTSRESFMPGVLLAIKEVMNRQELVVGLDKLF",
    "response#3": "MRVAVSGAAGRMGRLVVKNAVAEGLKVVQAFDINEVGKDAGELAGVGKIGVPIEDDISKLDADVLIDFTTAEAAMKNAEVAAEKGVRVVMGTTGFTDEDRKRLAELAEKVPMIVSPNFSVGAVLMMVLAAHAAPYFRRAEIIERHHENKRDRPSGTSLRTAALMNTARSQAWSVEPDSRELLEGVGGGDAGGVRVHSVRMPGSVAHQEVILGSPGETLTIRHDSIDRTSFMPGVILALKKVRSLSGLTIGLEHLLDL"
  },
  {
    "reference": "MKKVLRVYGGVMRLVRLLPADSRPYYAKYARENFVNYREVDQSETSLDELFQRAYNHSLWVLKKYSIDESAATKLKEICFE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein localizes to the cytosol. The designed protein sequence is ",
    "response#1": "ESEQIKTLNNKFASFIDKVRFLEQQNKLLETKWTLLQEQKSAKSSRLPDIFEAQIAGLRGQLEALQVDGGRLEAELRSMQDVVEDFKNKYEDEINHRTAAENEFVVLKKDVDAAYMSKVELEAKVDALNDEINFLRTLNETELTELQSQISDTSVVLSMDNSRSLDLDGIIAEVKAQYEEMAKCSRAEAEAWYQTKFETLQAQAGKHGDDLRNTRNEISEMNRAIQRLQAEIDNIKNQRAKLEAAIAEAEERGELALKDARAKQEELEAALQRGKQDMARQLREYQELMSVKLALDIEIATYRKLLEGEESRLSS",
    "response#2": "FTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSKNEEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRVI",
    "response#3": "ESEQIKTLNNKFASFIDKVRFLEQQNKLLETKWTLLQEQKSAKSSRLPDIFEAQIAGLRGQLEALQVDGGRLEAELRSMQDVVEDFKNKYEDEINHRTAAENEFVVLKKDVDAAYMSKVELEAKVDALNDEINFLRTLNETELTELQSQISDTSVVLSMDNSRSLDLDGIIAEVKAQYEEMAKCSRAEAEAWYQTKFETLQAQAGKHGDDLRNTRNEISEMNRAIQRLQAEIDNIKNQRAKLEAAIAEAEERGELALKDARAKQEELEAALQRGKQDMARQLREYQELMSVKLALDIEIATYRKLLEGEESRL"
  },
  {
    "reference": "MTRFIFVTGGVVSSLGKGIASASLAAILEARGLKVTMLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERYIRTKMTKRNNFTSGRVYEDVIRKERRGDYLGGTVQVIPHITDEIKRRVVEGASGYDVALVEIGGTVGDIESQPFLEAVRQLKIEVGSNRAMFMHLTLVPYIATAGETKTKPTQHSVKEMRSIGIQPDILVCRSDHEIDDSSRRKIALFTNVEERAVIPLQDANSIYLIPRMLHERGLDNIICERFNLECEQADLSEWDEVVEAEANPEHEVTIAMVGKYMELLDAYKSLIEALKHAGLRNRTKVKIRYVDSETIIDEGTKQLEDVDAILVPGGFGNRGVEGKIQTVKYARENKVPYLGICLGMQVAVIEFARHVAGLDGANSTEFDAKSDYPVVGLITEWKDQSGAVEQRSDSSDLGGTMRLGSQECHLEPGSNAANAYGKARVNERHRHRYEVNNNLLPKLKDAGLIVSGRSSDGALVEVVEVPDHPWFVACQFHPEFTSTPRDGHGLFEGFVKAAMKNKAK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should contain one or more Glutamine amidotransferase domains that are essential for its function.2. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.3. The protein should have a high affinity for Mg(2+), ATP, UTP, CTP.4. The Nucleophile; for glutamine hydrolysis, active site of the protein must be engineered to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).5. A protein with CTP synthase activity is needed to enhance 'de novo' CTP biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "MFITGGVVSSLGKGIAAASLAAVLEARGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFVCMTATRRNNFTTGRIYENVIRKERRGDYLGGTVQVIPHITDEIKRCIRLGAGEADIAMIEIGGTVGDIESLPFLEAIRQMGVELGRERSLFIHLTLVPFIKSSGELKTKPTQHSVKELRSIGIQPDILMCRSDREIPAEERRKIALFTNVEPKAVINVPDADTIYRIPLLLRAQGVDDIVARKLQSQLETNPNQELRQLLIDAYKSLNEAIVHAGIHTRTKVNIEFLDSEEIEEKGVALLAGADAILVPGGFGQRGFEGKILAAKYSRENDIPYLGICYGLHAAVIDIARNVANLEEANSTEVNPETIHPVIALITEWTDGNGDRISRNEDSDLGGTMRMGEQECKLSCDSMTFKIYGEPGVRERHRHRYEVNDGYVKALEKAGLRVAGRSVDGTLVEVIEFSDHPWFVACQFHPEFTSTPRQGHPLFKSFIDAYKSIFEALTHAALRLGHAAEIIPIDAEDENLTMELSQCDACLVPGGFGVRGWEGKIAAAKFCREQGIPYFGICLGMQVLVVEYARNVLNLDQANSLEMDPNTPHPIVYVMEGQDPLVATGGTMRLGAYPCLLKPGSKAHKAYNESSLIQERHRHRYEVNPDYIQSLEDHGLRIVGTCPPQGLCEIIEVSDHPWMIGVQFHPEFVSKLISPHPLFIAFIKSAVAEA",
    "response#2": "MFITGGVVSSLGKGIAAASLAAVLEARGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFVCMTATRRNNFTTGRIYENVIRKERRGDYLGGTVQVIPHITDEIKRCIRLGAGEADIAMIEIGGTVGDIESLPFLEAIRQMGVELGRERSLFIHLTLVPFIKSSGELKTKPTQHSVKELRSIGIQPDILMCRSDREIPAEERRKIALFTNVEPKAVINVPDADTIYRIPLLLRAQGVDDIVARKLQDALDYHGKPDPALRDSYISLTEALIHAGLRTRTRVNIQFIEATDVEKHGTSCLEGADAILVPGGFGERGIDGKMAAIQYAREREIPYLGICLGMQLAVVEYARNVAGLEGANSTEFDRSTPHPVVALIAEWQDQKGTVEQRSEDSKLGGTMRLGAQEVRLSHGSLAHQIYGTDTIMERHRHRYEFNNRYLQKLMNAGLRFSGFSRDGLVEVVELPNHPFFIASQFHPEFRSTPRDGHPLFTGFIAAKAG",
    "response#3": "MFITGGVVSSLGKGIAAASLAAVLEARGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFVCMTATRRNNFTTGRIYENVIRKERRGDYLGGTVQVIPHITDEIKRCIRLGAGEADIAMIEIGGTVGDIESLPFLEAIRQMGVELGRERSLFIHLTLVPFIKSSGELKTKPTQHSVKELRSIGIQPDILMCRSDREIPAEERRKIALFTNVEPKAVINVPDADTIYRIPLLLRAQGVDDIVARKLQLELGTSPAPDLSRWEDIRRRVLSPEGEVTIAMVGKYMELLDAYKSLIEAISHAGIKSHTKVNTRYIDAEDIEMQGVSLLAGVDAILVPGGFGERGTEGKIKTVQYARENKIPYLGICLGMQVAVIEFARNVAGWNDANSSEFDANSKHCVVGLITEWTDEAGHIEQRSEGSDLGGTMRLGAQQCRLQEGSLAHQCYQQDVITERHRHRYEVNNTFVEQLEAVGLKFSGRSMDGSLVEMVEIADHPWFVACQFHPEFTSTPRAGHGLFEGFVGAAVSGR"
  },
  {
    "reference": "MKGNTKTIILYVVLIAALILACLSLLSSRSSAESITYGEVLELFHDNKVVECSISSSNLLTIKTTETDANGNPKTYQLRLRDLLMFKADVDEYIDAQLDLPEEQRTLKSYVIEEPRQTSWLVSLLPYLIILVIGVAFWFYMANKLSDGERKMTAFSKARVKVPNRDKTRVYFSDVAGADEEKEELREVVEYLRDPAKYRRLGAKIPRGVLLVGPPGTGKTLLAKATAGEADVPFFSISGSDFVEMYVGVGASRVRDLFETAKKAPASIIFIDEIDAVGRRRGTGLGGGHDEREQTLNQLLVEMDGFGGHDGVIVIAATNRPDILDPALLRPGRFDRQITVNYPDIKGREDILKVHAKGKPFENDVDLKKIAQTTVGFTGADLANLLNEAALLAARKNKALIGMVDIEESLIKITVGTQKRNYKIKEEEKRKTAYHEAGHAILAHFLPTQDPVRQISIIPSGRALGYTLNPPVEDKYSVYKNELRENIAMLLGGRAAEEIVFGDISGGASNDIQRATQIARKMVTVYGMSDAVGTIHLGSEHSDDEVFLGRDFNTGKDYSEQTAAVIDSEIKKIIDEAYARAKKILSEHREKLDFIAAFLVENEIMDDEQFKAAMETDATMEEIVKIGEEKRRRSEEENKLREAAEAEARRRDEEEARRRAEEEKARNGENPFRPDI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.2. Ensure the presence of a signal peptide within the protein.3. The protein must exhibit the following characteristics: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins.4. The designed protein must have a active site that is structurally stable and resistant to denaturation.5. The designed protein should have zinc ion binding, ATP-dependent peptidase activity, metalloendopeptidase activity, ATP hydrolysis activity, ATP binding within plasma membrane to regulate protein catabolic process, proteolysis.6. The protein should be able to bind Zn(2+), ATP ligand in a variety of conditions7. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "VLLYGAPGTGKTLLARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKNSPAIIFVDEIDAVGRHRGAGMGGGHDEREQTLNQMLVEMDGFDISTNVIMIAATNRPDILDPALLRPGRFDRQIGVHFDVDLRRIARGTPGFSGADLANLANEAALLAARKGKRLIASGEFVAEDLVFDVNARTIARGTPGFSGADLANLVNEAALLAARRGRRVVTMREFEAINYEG",
    "response#2": "MVMSEEEKRLTAYHEAGHAIVGRLVPSHDPVHKVTIIPRGRALGVTMFLPLEDRHSYSKQRLESQMSSMFGGRVAEELIFGPEAVTTGASNDIQRTTELARNMVTKWGLSTRLGPQTFGEEDGEVFLGHSVTKHKVVSDETAHIIDEEVRAFVDRNYARATRLLKENIDKLHKMADALIKYETIGVEQIDDIAERNGRR",
    "response#3": "VLLIGPPGTGKTLLARAVAGEAGVPFFSMSASEFVEMIVGVGAARVRDLFKQAREHAPAIIFIDELDAIGRARGQIAIGGSSEQEQTLNQLLSEMDGFSSREGIIVLAATNQPDVLDKALLRAGRFDRRVIIQFADDPQGLSRDVDIEKIAQSTPGFSGADLANLINEAALLASRGGRKDISVENFEEFVLRNL"
  },
  {
    "reference": "MEEKDESEQSISAGRQEIRKRRRPSQPMVDKSQQTEVTEKKKQLSIPQSSGPKAALSIGNIPGSKLNYECHRVSSQLQQTWIKRKRVQDMADKSLQTETIAEEKKEEIKLVCEAVVPEEKPAAVEVGPEFPESVREVEVPPNRYSVQVKIDRSQQTTCTGDWTMMNFPQKEKLDKEQQTYFSESEIVVIGWPTNSFSKSKEGAQKRKSSGNIFLSEHPEFQPTTSSNEEIRRPSISRTVSISPTKKDSPVPLEDEKDVPVEVQPPAAEEISAEEQLPLAETTSEEVPVAVQPPPAEEPPLEAQPSPTEEAPGDEAPAKVEPTPAEEALSEKPPAEEALVEIQPSPVEEAAGDEAPAKVEATSSEETLLKEPLTEVQPPAAEEAPIQDTPELQLSPAVEAPAEEAPAEAEPPPAEEAPAEEAPEVQSPPAEEAPAEEPPEIQSPPAEEAPAEEPPEVQSPPAEEAPAEEAPEVQSPPAEEAPAEEPPEVQSPPAEEVPAGEPPEVQSPPAEEAPAEEAPEVQSPPAEEVPAEEALAEVEPPPAEEAPAGEPSEVQSPPAEEAPAEEAPEVQSPPSEEAPAEEAPEVQSVPAGQAPAEEPLEVQPPPAEDATEEEASEVQSLPTDEAPAEEGLGFQSPPADKAPEEEAPEVQSPPAEEAPAEEPPEVQSLPADEAPAEEATEEVQSPPTEESPAEEAPAELQPPSTEETTSEMVSVEKQPSLTEEPFITPISLEETSAEVLLPPFEQTPADEALVENVSPVDQAPKEADV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Target a Pro residues compositional bias in the protein's composition for improved properties.2. The protein must exhibit the following characteristics: May be involved in the later stages of fibrous sheath biogenesis and spermatozoa capacitation.3. The protein should have calcium ion binding and be active in sperm principal piece. The designed protein sequence is ",
    "response#1": "QTTLALLHPDICEEEKDDVLNVIHNEGFTILMQRQIVLSEEEARTVCKIHENEEYFDNLIGHMTSNHSYVLALRRENGVEYWKTLIGPKTIEEAYASHPQSLCVQFASGNFPTNQFYGSSSKAAAEKEIAHFFPPK",
    "response#2": "LASSCCSSNILGSVNVCGFEPDQVKVRVKDGKVCVSAERENRYDCLGSKKYSYMNICKEFSLPPCVDEKDVTYSYGLGSCVKIESPC",
    "response#3": "LASSCCSSNILGSVNVCGFEPDQVKVRVKDGKVCVSAERENRYDCLGSKKYSYMNICKEFSLPPCVDEKDVTYSYGLGSCVKIESPC"
  },
  {
    "reference": "MINKDNESAAGDYEMANSSSNFSNIRHKDPTSSNNSHTVGQNFASDSFRAMEPTELVSSDREAYSVDSLNEPPAVPELSVEDGLADDDSWVEELSQRGEDEDEDLSNATTTPTATDSEDAEGEGEGARRHGYMDREEDLRGYNRSAIDFTLHTIVEESCEESEVASMRADNEDVELEDEDLSERRRQRTLQHHHRLSASELEKYFFFGLGDGRVMSSIDTRGDDTASEVSSECSESLDSLPHEDQLLDTSGASDLASSRLEKYFLSGFMGFSGAEKQAESDESGGSVGSDSEGQPSPSQRRKRLVRARGTPRSHNSSLDNLLLPESDTLDATVTSAGGAAAATVEDTSESEAGCDDTVIHLANAGASDGSSSDTIKRKKQLRKRHESLDEKKLHDLEPSECRTPTPGSSGQVQMQSQAKKQQQQHHHSRDSGFVGSNDDLLKAAPDCEPPKSPTPALEQISEDREPVHSQISLARMDQPSTSAAAAVASTLSSQLKNLALPNLVRKDSFNNWSSDEETNLMMSKMRQFFKNLIVATANAQQSKPSTPNQGPSTTNVNTTPSSQRRLAKSRPAQLAYFENELTRLMKTVPGINDEQVREIVEYLSSEDTWSDSYDSSDYTSSDLEGGERKGQLKAQISASCQQIINKFEIDEEGDRGDGGLLDESQSVPMEALVYQKLVASFSKVAAGGEPETEATKAVEEAEPSTERSPQLFAKVMQHIGTRLVALMHEVSSGNETPTPSPQGQRHHRRLQAKISATTTEDEEDEVAE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be optimized for stability and expression in apical plasma membrane where it will participate in morphogenesis of embryonic epithelium, positive regulation of multicellular organism growth, cortical actin cytoskeleton organization, positive regulation of cell size.2. Prioritize Basic and acidic residues, Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "LMNDFWEMETISFPPDGSSITPAHHYNDFRFKVYAPIAFRYFRDLFGIAPDDFLMSMCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLWPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNSLMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGKQVC",
    "response#2": "LMNDFWEMETISFPPDGSSITPAHHYNDFRFKVYAPIAFRYFRDLFGIAPDDFLMSMCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLWPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNSLMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGKQVL",
    "response#3": "MCASPLRELSNPGASGSIFYLTTDDEFIIKTVQKKECEFLQKLWPGYYMNLSQNPRTLLPKFFGLYCFHYNSKNVRLVAMNNLLPSDIKMHCKYDLKGSSFRRKASKAERQKASPTFKDLDFAEHHPNGIFLETDKYNSLMSTIKRDCMVLESFQIMDYSLLVGIHNLDLAAKEKREERILNARAKLQRKESAAQGPPSLNPDDDAPEADQNQLQAVASYASIPGTSAGAALNRTRSMNRQRLVAHSTALESITADMDVPLEEDEDVPAGGIPARSENDERLILYIGIIDILQSYRLEKKLEHTFKSILYNGDTVSVCRPSFYAKRFQDAMGL"
  },
  {
    "reference": "MSFRLKGKFIFFATNNINKCNEARVLLADYGISVGMLRVKSLEIQSESLEEIAKTSVTEAFKRCHLPLIVEDAGLFIKALNGFPGPYASYVYKTIGNKGVLKLMENVEDREAKFQSAIAYYAEGLKSPICFKGEAVGEVTKVERKGKGKSGFGFDPIFKPVGSDKTFAEMTTAEKNRYSHRAKALRKFAEWYKHQQ",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+).2. The protein should have a high affinity for substrate.3. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.4. A protein with nucleotide binding, metal ion binding, XTP diphosphatase activity, ITP diphosphatase activity is needed to enhance nucleotide metabolic process, purine nucleoside triphosphate catabolic process in a specific context. The designed protein sequence is ",
    "response#1": "MSILSGKVVLVTGASSGIGKATALAFAREGAKLSLAARRQEKLDTLVAEIKAAGGDALAIPTDVTNSEDCRRVVDTAVKTLGGLDVMCNNAGIAGAMGPFEQMPLAEWQQMIDVNLTGTFLGMKHAIPYLKESKTAAVINTASVCGLLGGSGAAAYVASKHGVVGLTRVAAIENADDGIRCNALCPGGVDTPMVQNAIAGNPEAMAAFQANQAKNRMATPEEVADAALWLCSDEAKFVTGSAIAVDGGYTAQ",
    "response#2": "MEMILATKNEGKLVEIRAILEPYGITVRSLADFPDPPEVVEDGLTFLENSRKKASALSRATGLPTMADDTGLEVDALNGAPGVYSARYAGSKGDDAANNRKLLEELEGVPDDRRTARFRCVMALVRDGEEHLFTGACEGRILTAERGTGGFGYDPVFFLPELGKTFAELTMDEKNRISHRGRALKKLLVFLR",
    "response#3": "MTRLSEK"
  },
  {
    "reference": "MMGSPPAPPARRLGALAVFLLALFLAAPWGVDCGYNVASVAGSKNRLRARLELAGGGGGAAPELGPDVRRLSLTASLETDSRLHVRITDADHPRWEVPQDVIPRPSPDSFLAATRPGGGRVLSTATSDLTFAIHTSPFRFTVTRRSTGDVLFDTTPNLVFKDRYLELTSSLPPPGRASLYGLGEQTKRTFRLQRNDTFTLWNSDIAAGNVDLNLYGSHPFYMDVRSGGGGGGGAAHGVLLLNSNGMDVIYGGSYVTYKVIGGVLDFYFFAGPSPLAVVDQYTQLIGRPAPMPYWSFGFHQCRYGYKNVADLEGVVAGYAKARIPLEVMWTDIDYMDAYKDFTLDPVNFPADRMRPFVDRLHRNGQKFVVIIDPGINVNTTYGTFVRGMKQDIFLKWNGSNYLGVVWPGNVYFPDFLNPRAAEFWAREIAAFRRTLPVDGLWVDMNEISNFVDPPPLNAIDDPPYRINNSGVRRPINNKTVPASAVHYGGVAEYDAHNLFGFLEARATHDALLRDTGRRPFVLSRSTFVGSGRYTAHWTGDNAATWEDLHYSINTMLSFGLFGIPMIGADICGFGGNTTEELCSRWIQLGAFYPFSRDHSAIGTVRRELYLWESVARSARKALGLRYRLLPYLYTLMYEAHTTGAPIARPLFFSYPGDVETYGIDRQFLLGRGVLVSPVLEPGATTVTAYFPAGRWFSLYDFSLAVATKTGKRVTLPAPADTVNVHVAGGNILTLQQPALTSSRVRQSVVHLLVALADDGTATGDLFLD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be optimized for hydrolase activity, hydrolyzing O-glycosyl compounds. The designed protein sequence is ",
    "response#1": "LTGKTIVMPRRLVPDKQVDHAIQAFAKALPDHPGWRLRIFGDGPQMSRLRNLIQGLGLHDSVELLGPSQHMTEEWARASLTILPSQDGEAFPLVLLEAFAAGVPAVAYDIVTGPAEIIRHGEDGLLVPPNDVESLAEAISRLMGDEALLRSYGEKAQ",
    "response#2": "LTGKTIVMPRRLVPDKQVDHAIQAFAKALPDHPGWRLRIFGDGPQMSRLRNLIQGLGLHDSVELLGPSQHMTEEWARASLTILPSQDGEAFPLVLLEAFAAGVPAVAYDIVTGPAEIIRHGEDGLLVPPNDVESLAEAISRLMGDEALLRSYGEKAR",
    "response#3": "LTGKTIVMPRRLVPDKQVDHAIQAFAKALPDHPGWRLRIFGDGPQMSRLRNLIQGLGLHDSVELLGPSQHMTEEWARASLTILPSQDGEAFPLVLLEAFAAGVPAVAYDIVTGPAEIIRHGEDGLLVPPNDVESLAEAISRLMGDEALLRSYGEKAF"
  },
  {
    "reference": "MRLIRSGAAQVRGEAERRGRPSRVAMLSMHTSPMEQPGTGDAGGLNVYVVELSRQLAALGVEVEVFTRAVSSKLPTSAELLPGVTVRHVDAGPFEEIHREDLPAWLCAFTAALLRAEAGHEPGWFDVIHSHYWLSGQVGLAVAQRWGIPLVHTSHTLAKIKNGALAVGDRPEPPGRLLGEQEVIGGATRLLASTPDEYRHLIDLYDAASDRVDVVAPGVDLEVFRPGDMAQSRARVGVDPADDLLLFVGRIQPLKAPDLLLRAAAELLRRDPARRSRLTVAVVGGPSGSGLEQPDALVKLAAYLGISDRVRFQPPAPQQELVHWYRAATAVVVPSHSESFGLVALEAQACGTPVVAAAVGGLRTAVADGVSGLLVSGRTPAVYADALDRLLRQPRWRARLSAGAVAWAGGFGWSATAHGVLRSYRHALSPTAVAV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a low Km value for 1D-myo-inositol 3-phosphate + UDP-N-acetyl-alpha-D-glucosamine   = 1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside 3-   phosphate + H(+) + UDP.2. For general function, the protein need meet that Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2- deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.3. A protein with acetylglucosaminyltransferase activity is needed to enhance mycothiol biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "GGMNVYVLQTALQLARRGVAVDIFTRATSSSDEPVVSVTDGVTVRNIVAGPFEGLDKYDLPTQLCAFTAGVLRAEAVHEPGYYNLVHSHYWLSGQAGWLARDRWGVPLVHTAHTLAAVKNQTLAEGDRPEPALRGVGEQQVVDEADRLIVNTKDEADQLVSIHNADPARIDIVHPDVTPWPVDRTAARRRLGVAEDAVVLLFVGRIQPLKAPDLLLEAAARMLADDPALRDRLQVHVVGAPSGTGLEAPRQLEQLAAGLGIADLLRFLPPVHVELLAEHYRAADVAVVPSHNESFGLVALEAQACGTPVVAAAVGGLRTAVRDGVSGVLVEGRDPADYAAAIRAVLARREDRDSARASLGLDPGEIVVTFVGRIQPLKAPDVLLRAAAEVISRSPGLPLRILVVGGPSGTGLARPDVLIELARSLGITAQVTFLPPQAPERLADVYRASDLVAVPSYSESFGLVAIEAQACGTPVIAADVGGLGVAVRNGETGLLVQGHRTEDWAGALESLVTAPTRLAELAAQG",
    "response#2": "GGMNVYIVELAKRLAAIDIEVEIFTRATTGTLPPTVELAPGVLVRHVDAGPYEGLAKEDLPAQLCAFTHGVMGAWAGHRPGYYDLVHSHYWLSGHVGWLAAERWRVPLVHAMHTMAKVKNAALAEGDTPEPAARVIGEEQIVGAADRLIANTEEEADELVRHYGADPELVAVVHPGADAYRLDRTAARRRLGVAEDAVVLLFVGRIQPLKAPDLLLEAAARMLADDPALRDRLQVHVVGAPSGTGLEAPRQLEQLAAGLGIADLLRFLPPVHVELLAEHYRAADVAVVPSHNESFGLVALEAQACGTPVVAAAVGGLRTAVRDGVSGVLVEGRDPADYAAAIRAVLARREDRDSARASLGLDPGEIVVTFVGRIQPLKAPDVLLRAAAEVISRSPGLPLRILVVGGPSGTGLARPDVLIELARSLGITAQVTFLPPQAPERLADVYRASDLVAVPSYSESFGLVAIEAQACGTPVIAADVGGLGVAVRNGETGLLVQGHRTEDWAGALESLVTAPTRLAELAAQRSAARAEFGLPADAVVLGFVGRIQPLKAPDVLLKATAALLDRHPGLRERLVVLVVGGPSGSGLERPRALHELARSLGITDVVRFLPPQRGDALASVYRACDVVAVPSYSESFGLVALEAQACGTPVVAAAVGGLPVAVADGVSGLLVDGHETRRWADALASVVLSPGRRARLPLADLAAGGMNVYIAKTSAKLAELGTEVEIFTRATSSDIPPVAQLAPGVTVRSLLAGPFEGLDKNDLPSQICAFAAGALRVEARHDPGYYDVVHSHYWLSGQVGWLARQRWGVPLVHTAHTLAKVKNAALAEGDTPEPMVRVMGEEQVVAGADRLVANTEFEAADLIERYDADPEAVATIPP",
    "response#3": "GGMNVYIRELASALAEAGVEVEIFTRATSASQPAVEHPDPGVCVHNVLAGPTKKIPKEELPGLLHSMVEEIEQIRRRQPHGRYDVIHSHYWVSGIAGLELSELWGVPLVHTMHTMAKVKNLLLESGEQPEPRRREVGEHRIVDGAARLIANTSSEAAELVSHYGADYDRIDIAPPGQAAPERSRRELGIPLHTKVVAFVGRLQPFKGPQVLIKAVAALFDRDPDRNLRVIICGGPSGPNATPDTYRHMAEELGVEKRIRFLDPRPPSELVAVYRAADIVAVPSFNESFGLVAMEAQASGTPVIAARVGGLPIAVAEGETGLLVDGHSPHAWADALATLLDDDETRIRMGEDPRAWHDRLWPMVARGAAPAPGPAPAPAPAPAAPGQAAPGQAAPGQAAPGQAAPAPAPAPAAPAPAAPAAKAAARAEFGLRADEQVVAFVGRIQPLKAPDLLVRAAERLPGVRVLIVGGPSGSGLDEPTALQDLAVDLGIADRVTFLPPQTRERLAQVYRAADIVAVPSYSESFGLVAIEAQACGTPVVAAAVGGLPVAVADQRTGLLVPTHRTEDWADAIGELL"
  },
  {
    "reference": "MKVMEIKNNCWSWGTLLLGILMISGMAQQLWVTVYYGVPVWKEAKTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVELVNVTEDFNMWKNNMVEQMHEDVISLWDQSLKPCVKLTPLCVTLNCSDCDTANCSIGNNNTNATIKNNSATHNASREIKICSFNVTTDIRDKVKKEYALFHSLDVISIDKNNTSYRLISCNTSTLTQACPKVSFQPIPIHYCTPAGYALLKCKDKNFNGTGKCHNVSTVQCTHGIRPVVSTQLLLNGSLSEGDVIIRSENFTNNAKTIIVQLNETVKINCTRPNNNTRKSIHIAPGRTFFATGEIIGDIRQAHCNISRKEWNDTLKLIATKLREQYKNKTIVFQPPSGGDPEIVRHSFNCGGEFFYCDSTQLFNSSWNSNWSNNTEGSDNITLQCRIKQIINMWQQTGKAMYAPPISGIISCSSNITGLLLTRDGGNNQTNNNETFRPGGGNMKDNWRSELYRYKVVQIEPLGVAPTMAKRRVVQREKRAVGIGALFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTAVPWNNSWSKNKSLKEIWNNMTWMQWEKEIDNYTSLIYSLIEESQNQQEKNEQELLELDKWANLWSWFDISNWLWYIRIFIMIVGGLIGLKIVFAVLSVVNRVRQGYSPLSFQTLLPTQREPDRPGGIEGEGGEQDRGRSTRLVNGFLALIWDDLRSLCIFGY",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein contains novel Retroviral envelope protein GP41-like, Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains that confer a unique function or activity.2. For general function, the protein need meet that Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Transmembrane protein gp41: Acts as a class I viral fusion protein; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state3. Ensure the presence of a signal peptide within the protein.4. Target a Basic and acidic residues, Polar residues compositional bias in the protein's composition for improved properties.5. The ideal protein would be one that plays a critical role in actin filament reorganization, clathrin-dependent endocytosis of virus by host cell, virion attachment to host cell, positive regulation of receptor clustering through structural molecule activity within plasma membrane, viral envelope, host cell plasma membrane.6. The protein should contain a Helical membrane-spanning region.7. The protein design should include a Di-leucine internalization motif motif to ensure proper function. The designed protein sequence is ",
    "response#1": "MRVMGIRRNYQHLWGWGTMLLGMLMICSAAENLWVTVYYGVPVWRDAETTLFCASDAKAYDPEVHNVWATHACVPTDPNPQEVNLTNVTEEFNMWKNXMVEQMHTDIISLWDQSLKPCVKLTPLCVTLNCSDARTNNTNATIEGEIKNCSFNVNSRIRDKVKKEYALFYSLDIVPMDNDDNTSYGNTSYRLRSCNTSVITQACPKVSFEPIPIHYCTPAGFAILKCNDKKFNGTGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEVILRSENFTDNVKTIIVQLNESVMINCTRPNNNTRKSIPIGPGKAVYATGDIIGDIRQAHCNISVTQWNKTLEQVATKLREQFPNKTIVFNHSSGGDLEIVMHSFNCGGEFFYCNTTQLFNSTWNYTSTWNNTEETNETIILPCRIKQIINMWQEVGKAMYAPPIRGAINCSSNITGLLLTRDGGTDDPNDTETFRPGGGDMRDNWRSELYKYKVVQIEPIGLAPTKARRRVVQREKRAVGIGALFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNASWSNKSQKEIWENMTWLQWDKEISNYTDIIYHLIKESQNQQEKNEQDLLALDKWADLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSVINRVRQGYSPLSFRTHLPAPRGLDRP",
    "response#2": "MRVKGIRKNYQHWWRGGILLLGMLMIYSAAEEKLWVTVYYGVPAWRDADTTLFCASDAKAHSTEVHNIWATHACVPTDPNPQEIYLENVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTSANLTKVNNATTSNDFNPIIGNITDEVKNCSFNMTTELRDKKHKVYALFYSLDIVQIDNSSEYRLIKCNTSVIKQACPKISFDPIPIHYCTPAGYAILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENITNNVKTIIVHLNKSVEINCTRPSNNTRTSIAIGPGQLFYRTGDIIGDIRAAYCEINGTKWNKVLKQVTEKLKEHFKEKNITFQQPSGGDLEVITHHFNCRGEFFYCNTTRLFNNTCINNETIEGCQNITIPCKIKQIINMWQETGQAMYAPPIRGKISCISNITGILLTRDGGANKTNEEIFRPGGGNIRDNWRSELYKYKVVQIEPLGVAPTRAKRRVVEREKRAVGTLGAMFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNASWSNKSQKEIWENMTWLQWDKEISNYTDIIYHLIKESQNQQEKNEQDLLALDKWADLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSVINRVRQGYSPLSFRLTPNPGGLDRPGRIEEEDGE",
    "response#3": "MRVKEIRKNYQHLWRWGTMLLGMLMICSAADQLWVTVYYGVPVWRDAETTLFCASDAKAYDPEVHNVWATHACVPTDPNPQEVNLTNVTEEFNMWKNXMVEQMHTDIISLWDQSLKPCVKLTPLCVTLNCSDARTNNTSTLGKEAEMKNCSFNANTELKDKKHQEFALFYDLDIVQLEENNTEYRLITCNTSKISQACPKITFDPIPIHYCAPAGYAILKCNTKTFNGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSKNLTDNTKTIIVHLNQSIGIVCTRPGNNTRKSIRIGPGQAFYATNSIIGDIRKAHCNISKDLWNETLQKVTEKLEEHFPNKTIEFEPSSGGDPEITTHSFNCGGEFFYCNTSGLFGNLTESNSTNITLPCRIKQIINMWQGVGRAMYAPPIAGNITCNSNITGLLLTRDGGNQTEGKEIFRPGGGDMKDNWRSELYKYKVVEIKPLGIAPTEAKRRAVEREKRAVGIGAVFLGFLGAAGSTMGAASVALTVQVRQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLALERYLKDQQLLGIWGCSGKLICNTNVPWNSSWSNKNLTDIWENMTWMQWDKEINNYTSIIYSLLEESQNQQEKNEQDLLALDSWNNLWNWFDITNWLWYIRIFIMIIGGLIGLRIIFAVLSIVNRVRQGYSPLSLQTLLPVPRGPDRPEGIEEEGGERDRGRSGRLVNGFLPLIW"
  },
  {
    "reference": "MPLLLPSGTTLVAATHNPGKAKELVALLDGRFDILSAGALGLPEPDETESTFVGNALLKARHAAERSGKIAIADDSGLSVAALDGAPGIFSARWAGPSKDFAEAMAKVEERILETGSDDRSAWFTSALAVAWPGGPSVVVEGVVHGTLVFPGRGTRGFGYDPIFVPEGHDKTFGEMDPAQKDAISHRALAFAKLKAALFD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP2. The protein should be able to bind substrate, Mg(2+) ligand in a variety of conditions3. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+).4. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.5. The designed protein should be optimized for ITP diphosphatase activity, XTP diphosphatase activity, nucleotide binding, ribonucleoside triphosphate phosphatase activity activity in order to improve nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "MKSIVFATNNAHKLDEVREILGNRFKLLSLTDIGCNVDIPETADTFEGNAILKAEYVQEHFGLDCFADDSGLEVDALNGEPGVYSARYAGENCSYDQNIAKLLSEMKGKENRKARFRTVISLIVNDQEHLFEGIVNGTILQEKRGESGFGYDPIFVPDGYNKTFAELSMDEKNEISHRGRAVKALVDFLNDR",
    "response#2": "MKKVVIATNNQHKVSEFASIFSSKYNIELLSLNDIGCTQDIAETGNTFLQNSLLKAKYVSEVTKLIVLADDSGLEVDYLEGAPGIYSSRFAGENCSYEDNNNKLLSILKDVPDEKRTARFRCVIVYLQHAEDPTPIICQGIWQGMILEEKRGNGGFGYDSLFIPDGHEKTSAELSLEEKNTISHRGKALQKMMTILEEAM",
    "response#3": "MKLVFATNNQHKLEEVRQILGTKVELLSLQDIGCTEDIPETADTIEGNALLKSDYVTQKYGYDCFADDTGLEVEALNGAPGVYSARYAGEERTASDNMQKLLEEMKGKENRKARFRTVISLILDGKEHLFEGIVNGEIVREKRGEKGFGYDPIFMPEGYEQTFAEMSMEEKNKISHRGLAVAKLVDFLNKY"
  },
  {
    "reference": "MAVSANANGQEVSAAKKDAVRDKSVYSPVQTSRIDHTPTLPSVLQGNFKLNHGPPSTSAGQPEEIQKLFPCLFGQPSVQIVKSDSPSYNVDQKLKVGVVLSGGQAPGGHNVIAGFFDYLQAHAPGSVLYGFKGGPAGIMRNKVVEITAEFLYPYRNQGGFDIICSGRDKIETPEQFQQAVDTVTKLDLDGLVVIGGDDSNTNAALLGEYFRSKKVKCHVIGCPKTIDGDLKSVEVPISFGFDTACKIYAEMIGNIMVDARSTGKYYHFVRLMGRAASHITLECALQTHPNVAIIGEEVNSQNKTLREVTNSIADLVCQRAEMGKHYGVVLIPEGLIDFIPEIQHLISELNEILANQGCDEHGHWKSTLKPPSRALFDSLPHSIQEELLLERDPHGNVQVSRIEMEKMLISMIETELFARKHEGSYSGQFKGQAHFFGYEGRCGMPTNFDTTYCYALGYAAGALMHLGQTGLIASVANLTAPASEWTVGGTALTQLMDVERRNGKNKPVIKKAMVELNGAPFKKFASVRDAWAVEDSYVSPGPIQFAGPTSDDTNFTLKLELGAPVDV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. We require a protein that can specifically bind to the metabolites produced in the Carbohydrate degradation pathway.2. The Mg(2+), substrate binding site should be stable and able to withstand changes in environmental conditions.3. The protein requires Mg(2+) to activate a critical catalytic site.4. The designed protein should contain one or more Phosphofructokinase domains that are essential for its function.5. The Proton acceptor of the protein must be engineered to enable effective catalysis of beta-D-fructose 6-phosphate + diphosphate = beta-D-fructose   1,6-bisphosphate + H(+) + phosphate. The designed protein sequence is ",
    "response#1": "FESCLQLQEARTSFEEFRVPICVVPATISNNVPGTDYSLGTDTALNAIVETCDRIKQSASGTKRRVFIIETMGGYCGYLANMGALAAGADAAYIFEEQFDIRELQANVEHLTEKMKTSIQRGLVLRNENCNENYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAMQWVIGGESGMMRIGILTGGGDCPGLNAVIRAVVRGSQPSGDEVVGFRDGWRGVLEGDAVTMDLAAVRGILPRGGTVLGTSRTNPYAVEGGADRVLATLERFGIDALVPIGGEDTLGVAAKLAEAGVRVVGVPKTIDNDLDGTDYTFGFDTALGVATEAIDRLHTTGDSHHRTLVVEVMGRHAGWIALHAGMAGGATVVLIPELPFDVDAVVAHCQRRFDSGYSPIVVVSEGARPRDGDLVTATGERDPFGHVRLGGIGTALASLIEARTGRESRAVVLGHVQRGGTPSPFDRVLATRFGLAALDAAREGKWGHMVSLRGTEMVQVPLADVTGVLKTVDPRYYEEASVFFG",
    "response#2": "FESCLQLQEARTSFEEFRVPICVVPATISNNVPGTDYSLGTDTALNAIVETCDRIKQSASGTKRRVFIIETMGGYCGYLANMGALAAGADAAYIFEEQFDIRELQANVEHLTEKMKTSIQRGLVLRNENCNENYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAMQWALQGKSGVMVAIVHNKVLKLPLEAAVSVLKTVDMELFEIAETFFS",
    "response#3": "RIGVLTGGGDCPGLNAAIRAVTRRSIVSYDFDILGIKNGWDGLVNGEVEPLSLYSVSGILHKGGTILGTSRKNPFKNDEDVQKILDNVKKYDLQAIVAIGGEDTLGVAHKLSQHGLKFVGVPKTIDNDLQGTDYTFGFDTAVSIATEAIDRLHTTAESHHRVMVIEVMGRHTGWIAVMAGLAGGADCILIPEIPFTMDEVCELLMKRHQRGKNFSIVVVAEGAKPKDKDSVITRTAKVDEFGHVMLGGVGEYVGKEIEKRTGMETRVTILGHIQRGGTPTPFDRVLATRMGVKAADLVHEGKFGMMAALQGTDIVRVPLADATAKLKTVPRELYEEAGVFFG"
  },
  {
    "reference": "MAQKKGGGSTRNGRDSESKRLGVKVFGGQQIPAGSIIVRQRGTRFHPGTNVGIGKDHTLFALIDGKVQFGFKGALNKQVVSVVAAE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must be able to perform structural constituent of ribosome under specific conditions. The designed protein sequence is ",
    "response#1": "GFEEKVVTIRRVTKVTKGGRHFRFAAVVVVGDKKGQVGLGTGKANEVPEAIKKAVKEAKKNLIRVK",
    "response#2": "MDVKEVKMRYKIDEHDYQVRINQAKRFLKAGDKVKATVNFRGREIQHAHLAKELLDRMATDLATEADIQQAPKREGRNMMMFLSPV",
    "response#3": "GGRHFRFAAVVVVGDKKGQVGLGTGKANEVPEAL"
  },
  {
    "reference": "MKPALRLQLDRYVRRLAELDSLLADPAVMRDAGGFRALSREHVEVSGIVERQRRHAQRERDLAAAAEMASDPELAAMAAEEAASAQAELDRLATELQRLLLPRDPDDARSVFLEIRAGTGGDESALFAGDLLRMYTRYAERKGWRVELLSASDSELGGYKEVVARIEGDAVFGHLKYESGGHRVQRVPATETQGRIHTSACTVAVLAEPDEAQEVTLNPADLRIDTYRASGAGGQHVNKTDSAVRITHLPTGLVAECQDDRSQHRNKAKAMAVLAARLRDKDRLERQAREAATRKSLIGSGDRSDRIRTYNFPQGRLTDHRINLTLYQLGAIMDGDLDPVVKALVAAREAELLAELEIG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must be able to translation release factor activity, codon specific.2. For general function, the protein need meet that Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA. The designed protein sequence is ",
    "response#1": "NESIQENEARMAEPGFWDDQAAAQKVIDENNVLKGKYDTFKQLADEVGDLAVAYELLSEEPDAEMQAEFETDFQHAEHDLQQYRLNLLLDGPYDRNNAILEIHPGAGGTESQDWGAMLLRMYTRWAASHNFKVETVDYQAGDEAGIKSVTLLISGHNAYGYLRSEKGVHRLVRISPFDAAGRRHTSFASV",
    "response#2": "QRLGELDFLLSREDIMSDMAQYRTISREHAEITQIAGRYERYKQREADIAGAAEMLEDPDMAEMAREEIAAAQAELVQLEEELQRLLLPKDPDDARNAFLEIRAGTGGDESALFAGDLLRMYTRYCERAGWRCEVVSESESELGGYKEVVIRIAGNDVFGRLRFESGGHRVQRVPATETQGRIHTSACTV",
    "response#3": "QRLGELDFLLSREDIMSDMAQYRTISREHAEITQIAGRYERYKQREADIAGAAEMLEDPDMAEMAREEIAAAQAELVQLEEELQRLLLPKDPDDARNAFLEIRAGTGGDESALFAGDLLRMYTRYCERAGWRCEVVSESESELGGYKEVVIRIAGNDVFGRLRFESGGHRVQRVPATETQGRIHTSACTI"
  },
  {
    "reference": "MSKQNAICINIHMEQPYMTREEFAKKLDVSTRTIDRLRQQGVLKCIKMKNDEGEETERGLVLVDLVAIAVRNAKNAFQI",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Accessory protein needed to activate excisive recombination The designed protein sequence is ",
    "response#1": "MLTDAAFESGQTFQAELVEDDAARLAEIVDDFWRRRIVAAPLLAQVLVERGETPDAWLAEIRPYLSKPYLKLRAPDAVRLETAQRVACERWQALADEPALVEEGLALFLAHPGFKANIYRPDTMARAADNLRGWLAQPDALPELDSDSRKLLDKLRPEALAKGMKKGCDAPAHPLFERMEAWLAAWDAYMEQVAHSLAGLKLELIAWANEELARRRGVERSRSFDDLLTDLGAALDHPATGPLLAAQVADSFAVALIDEFQDTDPTQYRIFRRCFVEEARPVFLVGDPKQAIYSFRGADIFAYLAARHDAERQYTLDTNRRSR",
    "response#2": "YPVMEKSLQFWQAHDFARKLDALKPLFVEPRGKGSFSEVMDAYYARVAAPGSSGAALLAVCRGKASEGLDFADNNGRGVVITGLPYPPRMDPRVVLKMQFLDEML",
    "response#3": "YPVMEKSLQFWQAHDFARKLDALKPLFVEPRGKGSFSEVMDAYYARVAAPGSSGAALLAVCRGKASEGLDFADNNGRGVVITGLPYPPRMDPRVVLKMQFLDEMY"
  },
  {
    "reference": "MDPETCPCPTGGSCTCSGSCKCEGCKCTACKKSCCSCCPAECQKCAKDCVCKGDEGAQAEAEKCSCCQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Metallothioneins have a high content of cysteine residues that bind various heavy metals.2. A protein that contributes to protein stabilization, negative regulation of axon extension, positive regulation of DNA-templated transcription, positive regulation of vascular endothelial growth factor receptor signaling pathway by functioning in inclusion body, perinuclear region of cytoplasm with protein kinase activator activity, zinc ion binding, copper ion binding, cysteine-type endopeptidase inhibitor activity involved in apoptotic process, cadmium ion binding. The designed protein sequence is ",
    "response#1": "MSILSGLLSCACAGSCKCKECKCTSCKS",
    "response#2": "GMAS",
    "response#3": "MPSSPRRR"
  },
  {
    "reference": "MSGRLVLATSNTGKLAELQPLLAEAGFALVAQGELGVPDAEETGLSFVENALLKARQACEATGLPALADDSGLVVPALGGAPGLYSARYAGGHGDHAANIARLLAELAGRSGADRRAHFYCVLVLLRHARDPQPLIAEGTWDGRILEAPRGTGGFGYDPVFLPDGHERSAAELAPELKNRISHRGQAIARLRERLREI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor to enable effective catalysis of dITP + H2O = dIMP + diphosphate + H(+).2. The designed protein should have a binding site that is specific to the target Mg(2+) ligand.3. The protein design must be able to perform metal ion binding, nucleotide binding, ribonucleoside triphosphate phosphatase activity, ITP diphosphatase activity, XTP diphosphatase activity in order to contribute to nucleotide metabolic process.4. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.5. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions. The designed protein sequence is ",
    "response#1": "MVNDIIIYATNNPKKVEEIRDILGDKIKLLSLKDVGLDGEVIEDGDSFENNALKKAEEVYRYTGKGVIADDSGLMVDVLGGAPGIYSARYAGENATDKDNIDKLLDELAGKEDRGAKFVCCIALVEPDGEEKIVRGECEGKILDEPRGNGGFGYDPLFMVEGYNKTFAEMDEEEKNKISHRGKALKKMKDILSDIL",
    "response#2": "MDYILGIDPGSRITGYGVIRQVGRQLVYVGSGCIRTSSADLPSRLKLIYAGVTEIITQFNPDCFAIEQVFMAKNADSALKLGQARGAAIVAAVNRDLPVFEYAARQIKQTVV",
    "response#3": "MNIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSELGKQAKDIMDAGELVSDELVIALVKERIAQDDCKNGKNGV"
  },
  {
    "reference": "MVPAAGALLWVLLLNLGPRAAGAQGLTQTPTEMQRVSLRFGGPMTRSYRSTARTGLPRKTRIILEDENDAMADADRLAGPAAAELLAATVSTGFSRSSAINEEDGSSEEGVVINAGKDSTSRELPSATPNTAGSSSTRFIANSQEPEIRLTSSLPRSPGRSTEDLPGSQATLSQWSTPGSTPSRWPSPSPTAMPSPEDLRLVLMPWGPWHCHCKSGTMSRSRSGKLHGLSGRLRVGALSQLRTEHKPCTYQQCPCNRLREECPLDTSLCTDTNCASQSTTSTRTTTTPFPTIHLRSSPSLPPASPCPALAFWKRVRIGLEDIWNSLSSVFTEMQPIDRNQR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.2. The designed protein should be expressed specifically in the extracellular region3. For general function, the protein need meet that Involved in control of cellular proliferation The designed protein sequence is ",
    "response#1": "FMESLNRLKEIHEKEVLGLQNKLLELNSERCRDAQRIEELFSKNHQLREQQKTLKENLRVLENRLRAGLCDRCMVTQELARKRQQEFESSHLQNLQRIFILTNEMNGLKEENETLKEEVL",
    "response#2": "FMESLNRLKEIHEKEVLGLQNKLLELNSERCRDAQRIEELFSKNHQLREQQKTLKENLRVLENRLRAGLCDRCMVTQELARKRQQEFESSHLQNLQRIFILTNEMNGLKEENETLKEEVF",
    "response#3": "FMESLNRLKEIHEKEVLGLQNKLLELNSERCRDAQRIEELFSKNHQLREQQKTLKENLRVLENRLRAGLCDRCMVTQELARKRQQEFESSHLQNLQRIFILTNEMNGLKEENETLKEEVL"
  },
  {
    "reference": "MQRITVLGATGTIGVNTLDVIARHPERFSVFALTGHRQVDRLAGQCRQFRPQVAVVADAAAAGELARQLSGMNVEILHGEAALVAVAASGSVDAVMSAIVGAAGLAPTMAAVRAGKQVYLANKESLVVAGRLMMEAVAASGSRLLPIDSEHNAIFQSLPADFSGDLDASGVERIVLTASGGPFRGWSAQQLAHVTPEQAVAHPNWVMGRKISVDSASLMNKGLEVIEARWLFNAPPGRIEVIVHPQSVIHSMVRYRDGSVVAQLGVPDMRTPIAHALAWPERMDAGVPALDFSLLGGLTFEKPDQEHFPCLGLAFDALREGGDQPAVLNAANEVAVAAFLEKRLAFMDIPRLVQKAMHQFAGRVSNSIEELLELDAEVRRHLLCSLAN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Catalyzes the NADPH-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4- phosphate (MEP).2. The designed protein should have NADPH binding, metal ion binding, 1-deoxy-D-xylulose-5-phosphate reductoisomerase activity to facilitate isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway, terpenoid biosynthetic process.3. The protein should be able to bind 1-deoxy-D-xylulose 5-phosphate, Mn(2+), NADPH ligand in a variety of conditions4. A protein that is capable of catalyzing reactions within the isopentenyl diphosphate biosynthesis via DXP pathway, isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 1/6, Isoprenoid biosynthesis pathway with high efficiency and specificity.5. The protein requires Mg(2+), Mn(2+) to activate a critical catalytic site.6. The protein should have a fast turnover rate for 2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-   xylulose 5-phosphate + H(+) + NADPH. The designed protein sequence is ",
    "response#1": "MLGSTGSIGTQTLEIVSELPDKFKVVALSAGRNIDLLTEQVSQHKPEVIAIEDENLLTDLKNNINNLGISNPPIVLGGREAINSVAAWDSADTVITGIVGCAGLIPTMSAIKAGKNIALANKETLIAAGQLFVDQVKAAGAKLLPIDSEHNAVFQALPQGYARDPQRCGVRRVLLTASGGPFRERSIESLADVTPDEACAHPNWVMGRKISVDSATMMNKGLEVIEAHWLFGAEYDAIEVVVHPQSVVHSMVEFRDGSVLAQLGSPDMRLPIQFALTYPDRKQNNFSKLDIVKIGSLTFEAPDLEAFPCLGLAFEALRAGGTMPAVLNAANEKAVGLFLQEKIRFLDIPEIIEKVMGRHSVKPDPDIDDIIDVDLWARRAAEDLVR",
    "response#2": "MLGSTGSIGTQTLEIVSELPDKFKVVALSAGRNIDLLTEQVSQHKPEVIAIEDENLLTDLKNNINNLGISNPPIVLGGREAINSVAAWDSADTVITGIVGCAGLIPTMSAIKAGKNIALANKETLIAALGNPDMRTPIAHALAYPERIDAGVRPLDLFEIGRLNFERPDFVRFPCLALAYDALREGGAAAAVLNAANEEAVAAFLERRVGFTRIPDIIAATLERARDLSVDCIEAILDADARARGALDNGGNHIIPVDSEHNAIFQCLPQAAQQQVGTMALAEHGIQYLLLTGSGGPFRDIPLEELPQQTPGAACNHPNWSMGRKISVDSATMLNKGLEYIEARWLFGMSPDHVELVLPQTLGAPDMRIPIAHCLGFPDRITGRAAKLDLAKIGQLTFEAPDFTRFPGLRLAYDALRTGNGATTVYNAANEVAVAAFIAQKIRFGAIARLVEDTLNSWVRAGNQAPLGSADDAIALDQQARKLAEAVA",
    "response#3": "MLGSTGSIGTQTLEIVSELPDKFKVVALSAGRNIDLLTEQVSQHKPEVIAIEDENLLTDLKNNINNLGISNPPIVLGGREAINSVAAWDSADTVITGIVGCAGLIPTMSAIKAGKNIALANKETLIAAGRLFMDAVRGGGARMLGSTGSIGTQTLEIVSELPDKFKVVALSAGRNIDLLTEQVSQHKPEVIAIEDENLLTDLKNNINNLGISNPPIVLGGREAINSVAAWDSADTVITGIVGCAGLIPTMSAIKAGKNIALANKETLIAAGRLYMESVRAGARVIPVDSEHSAIFQVLQSECAHRVSRLILTASGGPFRTWDKAAMARATPEQAIAHPNWSMGAKISVDSATMMNKGLEMIEASYLFGLQHDQIKVLIHPQSVIHSMVRYMLGSPDMRLPIQFALTYPDRKQNNFSKLDIVKIGSLTFEAPDLEAFPCLGLAFEALRAGGTMPAVLNAANEKAVGLFLQEKIRFLDIPEIIEKVMGRHSVKPDPDIDDIIDVDLWARETAEKMAAEFLR"
  },
  {
    "reference": "MRRFLFLSLGVLVVAFSLNGIGADHHCPWDWFSFDRFCYKVIKQRKNWQDAERFCGQLQNGSHLASIQSWAESKYVAMLLSNNAFLDTVWIGLFEPEKNRSLEWSDGSGFCYTGWERRKRNNVDNKKYCVELSWMSDYLEWNNVKCESRNIFICKI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design must feature the Sugar-binding motif.2. For general function, the protein need meet that Lectin which recognizes specific carbohydrate structures and agglutinates a variety of animal cells by binding to cell-surface glycoproteins and glycolipids; May be a calcium-dependent lectin .3. Design the protein with a signal peptide incorporated.4. The protein should be able to bind Ca(2+) ligand in a variety of conditions5. The designed protein must have at least one C-type lectin domain.6. The designed protein must be optimized for metal ion binding. The designed protein sequence is ",
    "response#1": "LPADFAVKNKKFTIGTLGVGSFFRAWRNCIDEGKGLATIESEKEQKYLESLLKASSTGSNYWIGATNIGASNTNKLTWITTDLPVQTKPPFLNVVAKSTCIALTPTGSWTLRNCLNPLNIFPYICK",
    "response#2": "LPADFAVKNKKFTIGTLGVGSFFRAWRNCIDEGKGLATIESEKEQKYLESLLKASSTGSNYWIGATNIGASNTNKLTWITTDLPVQTKPPFLNVVAKSTCIALTPTGSWTLRNCLNPLNIFPYICR",
    "response#3": "LPADFAVKNKKFTIGTLGVGSFFRAWRNCIDEGKGLATIESEKEQKYLESLLKASSTGSNYWIGATNIGASNTNKLTWITTDLPVQTKPPFLNVVAKSTCIALTPTGSWTLRNCLNPLNIFPYICKK"
  },
  {
    "reference": "MNMDKFEQVTVLKAANIYYDGKVSSRVVQFQDGSRKTLGIMLPGQYEFSTDTQEVMEIVSGQLRVLLPDATEWLEIDGAGEFTVPAGTTFKLDIRTVTDYCCSYA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Also catalyzes the reverse reactions.; Catalyzes the phosphorolysis of diverse nucleosides, yielding D-ribose 1-phosphate and the respective free bases; Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates.2. The protein should have a low Km value for phosphate + xanthosine = alpha-D-ribose 1-phosphate +   xanthine.3. The protein must be able to pyrimidine-nucleoside phosphorylase activity, purine-nucleoside phosphorylase activity. The designed protein sequence is ",
    "response#1": "MNIFENPVSLGLA",
    "response#2": "GVPLI",
    "response#3": "MIALDGLKILITGASRGIGRATALAFAREGADIVAFARDRDALDTLRKEIEAAGGRAVAVPGDVTKAAGEAAVIEAALAQLDGLDIAVANAGIAPTGTAAALTDAEWRRVIDVNLTGVFLTCRAELRRMLERGRPGSIVLVA"
  },
  {
    "reference": "MVKACEFCHEKHLHCDPGRPCINCVKRNRGQLCRDKERKKSKKSGVEKSVGGSDAQSSGSSQSNNTAEVNVSADYTAGTMFNNMGFDPLQQSNYGFDTMTRQNNNNDNTLLNSSNNNAIDMGNRWSSQPDVVDNMVSPNITDGSNTVEFDSAWANNEYMKLDELTKNGPPMMFSSYPNSVTENLDQDNAVEDLPISVLPGSKTKNGTAENLSQFLNIGRPLHKTQSRPYISLDMMTAGMKDDILPPETYNTTSLGDNYLSPINSVGSSTNQLNDYQDSIPHQKTMTSPVLPTQSPSSAFNPAKLPTQVSISAISPDDNNEDDQKSSSGIPSKSDKEKNNKTKKAKEKIFKTSPNDILISKKNPKGHKSTKRKNEKADISPYKFRQLVKVPEDLYEKQYLIKPHNYRHTYKKLLEQLEKIFLSDNSPKRRGQLQSIVKCILEDYVPTFIALTSNMIESDLYLQELTLQRTLLELESMAKLVNCSPICIWRRSGEISYVSDEFLQLTGFKRKEILASRRFIFEFLDPTSIVNYFTNFHEYLAFGSKNQMTSNISLIKQGLLVNGAVVRKSNPIVSSSPMCGTNTDGIFDECHLLLSNGYYLKCATCWTVKRDSFNIPLLIMGQFLPVFEGQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should emphasize Polar residues compositional bias.2. The designed protein should be expressed specifically in the nucleus The designed protein sequence is ",
    "response#1": "TTQVPYRINFLPDDSQNAQLPPPMEEVGICGIKQVIRAFGISQEVATLGISLYVLGFTFGPLIWAPLSELYGRKKVFFFTFMVATAFSAGAAGAGSIASLLVLRFLTGSIGSAPLSNAPALIADMFDKSERGLAMCMFSGAPFLGPAIGPIAGGFLGETAGWRWLHGLMAAFTGVTWIACTVFIPETYAPYILRKRAQHMSKLTGKVYISTLDADKPPSSAAHQLKNALTRPWLLLFKEPIVFITSIYISIIYGTMYMCFAAFPIVFQQGRGWSQGIGGLAFTGIVIGVILSIISFAFEDKRYARAAQRRGAPMEPEDRLPPAIMGSLLIPIGLFWFAWTTFASIHWIVPIIGTVFFAWGLVLVFMALLNYLIDSYVIFAASIMAANSALRSLFGAAFPLFTRQMYDGLGVQWASSIPAFLALACVPFPFLFYKYGL",
    "response#2": "TTQVPYRINFLPDDSQNAQLPPPMEEVGICGIKQVIRAFGISQEVATLGISLYVLGFTFGPLIWAPLSELYGRKKVFFFTFMVATAFSAGAAGAGSIASLLVLRFLTGSIGSAPLSNAPALIADMFDKSERGLAMCMFSGAPFLGPAIGPIAGGFLGETAGWRWLHGLMAAFTGVTWIACTVFIPETYAPYILRKRAQHMSKLTGKVYISTLDADKPPSSAAHQLKNALTRPWLLLFKEPIVFITSIYISIIYGTMYMCFAAFPIVFQQGRGWSQGIGGLAFTGIVIGVILSIISFAFEDKRYARAAQRRGAPMEPEDRLPPAIMGSLLIPIGLFWFAWTTFASIHWIVPIIGTVFFAWGLVLVFMALLNYLIDSYVIFAASIMAANSALRSLFGAAFPLFTRQMYDGLGVQWASSIPAFLALACVPFPFLFYKYGLKFE",
    "response#3": "TTQVPYRINFLPDDSQNAQLPPPMEEVGICGIKQVIRAFGISQEVATLGISLYVLGFTFGPLIWAPLSELYGRKKVFFFTFMVATAFSAGAAGAGSIASLLVLRFLTGSIGSAPLSNAPALIADMFDKSERGLAMCMFSGAPFLGPAIGPIAGGFLGETAGWRWLHGLMAAFTGVTWIACTVFIPETYAPYILRKRAQHMSKLTGKVYISTLDADKPPSSAAHQLKNALTRPWLLLFKEPIVFITSIYISIIYGTMYMCFAAFPIVFQQGRGWSQGIGGLAFTGIVIGVILSIISFAFEDKRYARAAQRRGAPMEPEDRLPPAIMGSLLIPIGLFWFAWTTFASIHWIVPIIGTVFFAWGLVLVFMALLNYLIDSYVIFAASIMAANSALRSLFGAAFPLFTRQMYDGLGVQWASSIPAFLALACVPFPFLFYKYGRELD"
  },
  {
    "reference": "MAKTKYEVTYIIKPDIDEDSKKALIDRFDKVVTDNGAEELESKDWGKRRFAYEIEKYREGTYHIMTFVAENSEPVDEFGRLSRIDNQILRSMTVKLDK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be optimized for ribonucleoprotein complex, ribosome-specific folding and stability, while maintaining structural constituent of ribosome, rRNA binding.2. For general function, the protein need meet that Binds together with S18 to 16S ribosomal RNA. The designed protein sequence is ",
    "response#1": "PRKGPVSKRSVLPDPKFGSHLMTKFINRLMHDGKKGVAENLFYQAVEVLAEKSGEDPLKAFEKAIGNVKPHMEVKPRRVGGATYQVPMEVRPERQVTLALRWLINQARARGEKGMADKLSGELLDAYHNRGGAVKKKEDTHRMADANR",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#3": "PRKGPVSKRSVLPDPKFGSHLMTKFINRLMHDGKKGVAENLFYQAVEVLAEKSGEDPLKAFEKAIGNVKPHMEVKPRRVGGATYQVPMEVRPERQVTLALRWLINQARARGEKGMADKLSGELLDAYHNRGGAVKKKEDTHRMADANR"
  },
  {
    "reference": "MPSKGKDKKKGKSRGKDTKKLIKTDESVVDRAKANASLWEARLEITELSRIEYRDTSRTLAKSNEDLKKKQCKMEKDIMSVLSYLKKQDQEKDNMIEKLKQQLNETKEKAQEEKDKLEQKYTRQIDELEGQFHQKAKEIGMIHTELKAVRQFQRRKIQVERELDDLKENLRNTERIHQETLRRLESRFFEEKHRLKQEAEKKIIMLAERAHHEAVVQLNDAGRNVFKENVYLQKALAYHLKEAGGLQKNSQKLQESHALLLHQKEINDLLIKEKIMQLVQQRSQIQTLQKKVVNLETALGYMTKEFESEVLKLQQHAMIENQAGQVEIDKLQHLLQMKDREMNRVKKLAKNILDERTEVERFFLDALHQVKQQILISRKHYKQIAQAAFNLKMRAACAGRTEYPKIRTFDGREHSTNSVNQDLLEAEKWTHIEGNVDIGDLTWEQKEKVLRLLFAKMNGCPSRKYNQSSKPPVPDYVVSDSGETKEFGDESKLQDKIFITQQIPISDSSGKVVLPTIPKGPQESDTGTF",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in motile cilium assembly.2. The protein must exhibit the following characteristics: May act by mediating a maturation step that stabilizes and aligns cilia orientation; Not required to respond to planar cell polarity (PCP) or flow-based orientation cues .; Basal body protein required in multiciliate cells to align and maintain cilia orientation in response to flow. The designed protein sequence is ",
    "response#1": "RERCEELRTKLASIHEKKVVEERNAQIEFNKELKRQKLVEEHLFARLWEEDRLAKERREAQEEKRQRELVQNTRLGLDAQVTSIQAQRQGARRMKEEEARILEQNKAQIKREDEQEKLQKQKRRQETRSSLKKAVQDKIESMQREYREDLDLNMKLVGRALQDLQDEADKKKQKREEMGREQKIYNDYLMQRREEEKAQEKELNRLLEDIKAKKLAEKDRELALQRAARKQLMNEVMNTRKLQVQERLQRKLREQEELALHEQRISESLKVLHQEDMEDFARRCALAEEYRNQLQMQIAHQQQAREAEKEEERQEFEAGLAANKACLDKIQRR",
    "response#2": "DIMSVLSYLKKQDQEKDNMIEKLKQQLNETKEKAQEEKDKLEQKYTRQIDELEGQFHQKAKEIGMIHTELKAVRQFQRRKIQVERELDDLKENLRNTERIHQETLRRLESRFFEEKHRLKQEAEKKIIMLAERAHHEAVVQLNDAGRNVFKENVYLQKALAYHLKEAGGLQKNSQKLQESHALLLHQKEINDLC",
    "response#3": "ILGSSLFYKGYLVCSHLPKDDIVEIAAYCRQYCLLPLAAKQRIGQLIIWREVFPQHHLQPPADSDTEVFQEPKGRYFLLIVGLRHYMLSVLLEAGGCTSKATGNPGPDCIYVDQARTTLL"
  },
  {
    "reference": "MNTPTHTHPHPFGSTATIRCERAAAELRTGRPVLLIDAHARRHAVMALDSMTAQSFATFANAVGNTHYLFLTPVRSNVLGLEAPQGARIPLATLSYDSLAKLAYLRQPTRPTAWAPGDIMDAAATEITRLALLLPAIVAAPLTQHTEHAFADCQTLDLADLDTAAACASTTEYELVTRTPVPLRDLGMSEFIVFRGGIAQRDQVAILIGRPDLSSAVPVRVHSSCLTGDLFGSLKCDCGDQLRHSLATLKALGGGVLLYLDQEGRGNGIAAKIRAYGYQHVGLDTIDADAQLGFGPDERRYTGAVMMLRALGITRIQLLSNNPAKAERLRAAGIIVKQRISVIGQITKQNEHYLRTKVSRAGHDLDIDALIMTSQRPQDPSETVDGETVKPIAKTGHA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that is capable of catalyzing reactions within the 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway with high efficiency and specificity.2. A protein with GTP cyclohydrolase II activity is needed to enhance riboflavin biosynthetic process in a specific context.3. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "LTTSMPTEKGNFSLHLYQDKIDNGYHLALVMGDKDFLSREPVLIRVHSQCLTGDIFHSLRCDCGAQLSRAMEMIAQEGKGVILYMNQEGRGIGLVEKLKAYALQDKGLDTVEANEALGYEPDLREYGIGAQILFDLGVRKIKLLTNNPRKIIGLEGYGLEVTERVR",
    "response#2": "LTTSMPTEKGNFSLHLYQDKIDNGYHLALVMGDKDFLSREPVLIRVHSQCLTGDIFHSLRCDCGAQLSRAMEMIAQEGKGVILYMNQEGRGIGLVEKLKAYALQDKGLDTVEANEALGYEPDLREYGIGAQILFDLGVRKIKLLTNNPRKIIGLEGYGLEVTERVI",
    "response#3": "LTTSMPTEKGNFSLHLYQDKIDNGYHLALVMGDKDFLSREPVLIRVHSQCLTGDIFHSLRCDCGAQLSRAMEMIAQEGKGVILYMNQEGRGIGLVEKLKAYALQDKGLDTVEANEALGYEPDLREYGIGAQILFDLGVRKIKLLTNNPRKIIGLEGYGLEVTERVV"
  },
  {
    "reference": "MDPYSLTTEGLAVLVEELGQPGYRVEQIRSWLVRGVDDPQEMTDLPKDLRAILAERFAPARPELIDHRVADDGHTHKLLLRFPDGEAIETVLMLYPRRATVCISTQAGCALGCPFCATGQAGFRRQLTAGEVIRQVVVADAALRSGAIAGEHLPTDSPDHITNVVYMGMGEPLANLPATLASVLWLHDPDGFNLSARGITVSTVGLVPGIKRLQELDLPVTLAVSLHAATDELRDELVPINVTHPLAELEQAVRDYRAATNRRASIEWCLIGEVNDSDLQADRLADIAKRLRAHVNVIPMNPTPGVRWKEPSGTRTRAFVDRVRARGVEITLRDTRGRDADAACGQLLASYTLGGGSRLPAAVGAAERVDALAPDETAP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.2. The designed protein must have at least one Radical SAM core domain.3. A key design requirement for the protein is the optimization of the S-methylcysteine intermediate, Proton acceptor to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.4. The [4Fe-4S] cluster, S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions.5. The protein must be engineered to have a high affinity for [4Fe-4S] cluster in order to increase its catalytic activity.6. A protein that contributes to rRNA base methylation by functioning in cytoplasm with tRNA (adenine-C2-)-methyltransferase activity, tRNA binding, 4 iron, 4 sulfur cluster binding, metal ion binding, rRNA (adenine-C2-)-methyltransferase activity. The designed protein sequence is ",
    "response#1": "MTDHPAISDVSDAALLAWLRKRGEPAYRAKQIRRHATHGTAAEFAELTDLPKQLREALAASFSWTSVDPVREDASADGETRKALLRLADGHHIETVLMPHHGARNAVCVSTQAGCPMACAFCATGEMGLVRNLTAGEIVDQVRHWQRELAQRGERVSHVVFMGMGEPFNNYDATLASTRALIDPEAFGISPRRLTISTCGVVTGIDQLAGEGLPINLAVSLHAPDDPTRARIMPINRKWGVEEVLAAAARYVKRTKRRVTFEYVLLAGVNDSEETARELAALITRHGPTKDAHVNLIPYNPGAGGFRRPSVERMERFAALLQEHGIAATLRISKGQDIAAGCGQLKVAAR",
    "response#2": "MHPAISDVSDAALLAWLRKRGEPAYRAKQIRRHATHGTAAEFAELTDLPKQLREALAASFSWTSVDPVREDASADGETRKALLRLADGHHIETVLMPHHGARNAVCVSTQAGCPMACAFCATGEMGLVRNLTAGEIVDQVRHWQRELAQRGERVSHVVFMGMGEPFNNYDATLASTRALIDPEAFGISPRRLTISTCGVVTGIDQLAGEGLPINLAVSLHAPDDPTRARIMPINRKWGVEEVLAAAARYVKRTKRRVTFEYVLLAGVNDSEETARELAALITRHGPTKDAHVNLIPYNPGAGGFRRPSVERMERFAALLQEHGIAATLRISKGQDIAAGCGQLKVAGGPVA",
    "response#3": "MHPAISDVSDAALLAWLRKRGEPAYRAKQIRRHATHGTAAEFAELTDLPKQLREALAASFSWTSVDPVREDASADGETRKALLRLADGHHIETVLMPHHGARNAVCVSTQAGCPMACAFCATGEMGLVRNLTAGEIVDQVRHWQRELAQRGERVSHVVFMGMGEPFNNYDATLASTRALIDPEAFGISPRRLTISTCGVVTGIDQLAGEGLPINLAVSLHAPDDPTRARIMPINRKWGVEEVLAAAARYVKRTKRRVTFEYVLLAGVNDSEETARELAALITRHGPTKDAHVNLIPYNPGAGGFRRPSVERMERFAALLQEHGIAATLRISKGQDIAAGCGQLKVAEPALS"
  },
  {
    "reference": "MRILNVSLLVLASSLVAFVECGRDFYKILGVAKNANANQIKKAYRKLAKELHPDRNQDDEMANEKFQDLSSAYEVLSDKEKRAMYDRHGEEGVAKMGGGGGGGHDPFSSFFGDFFGGGGGHGGEEGTPKGADVTIDLFVTLEEVYNGHFVEIKRKKAVYKQTSGTRQCNCRHEMRTEQMGQGRFQMFQVKVCDECPNVKLVQENKVLEVEVEVGADNGHQQIFHGEGEPHIEGDPGDLKFKIRIQKHPRFERKGDDLYTNVTISLQDALNGFEMEIQHLDGHIVKVQRDKVTWPGARLRKKDEGMPSLEDNNKKGMLVVTFDVEFPKTELSDEQKAQIIEILQQNTVKPKAYNGL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Include a signal peptide as an essential component of the protein.2. The designed protein should be involved in protein maturation, protein folding by localizing to endoplasmic reticulum and exhibiting unfolded protein binding.3. The protein contains novel J domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "NYVMHQTLSAYYYLMKPSLAPSKVMEVFTKAAEYMEIPVRRTDDEPLQKLFMSVRSELNLDLKNMKQEQAKFWKQHPAIVKTELLIQAQLTRESGVLSPALQGDFRRVLELAPRLLEELLKMAVIPRTAQGHGWLRPAVGVVELSQCIVQAVPLSARKSSGVSSEGISPFLQLPHFSDAVVKKIARKKVKSFQDLQEMRLEDRSELLTQVAGLSATDVEDIEKVLEMMPSITVDITCETEGEEGIQEGDIVTLQAWVTLKRPNGLVGALPHAPYFPFHKEENYWVLLADSVSNNVWFSQKVSFLDEGGAITAASKAISESMEGSGAGVKETNDAVREAIEKVKGGSRLVMGKLQAPAEGTYNLTCFCLCDTWIGCDKKQAC",
    "response#2": "NYVMHQTLSAYYYLMKPSLAPSKVMEVFTKAAEYMEIPVRRTDDEPLQKLFMSVRSELNLDLKNMKQEQAKFWKQHPAIVKTELLIQAQLTRESGVLSPALQGDFRRVLELAPRLLEELLKMAVIPRTAQGHGWLRPAVGVVELSQCIVQAVPLSARKSSGVSSEGISPFLQLPHFSDAVVKKIARKKVKSFQDLQEMRLEDRSELLTQVAGLSATDVEDIEKVLEMMPSITVDITCETEGEEGIQEGDIVTLQAWVTLKRPNGLVGALPHAPYFPFHKEENYWVLLADSVSNNVWFSQKVSFLDEGGAITAASKAISESMEGSGAGVKETNDAVREAIEKVKGGSRLVMGKLQAPAEGTYNLTCFCLCDTWIGCDKKQAC",
    "response#3": "NYVMHQTLSAYYYLMKPSLAPSKVMEVFTKAAEYMEIPVRRTDDEPLQKLFMSVRSELNLDLKNMKQEQAKFWKQHPAIVKTELLIQAQLTRESGVLSPALQGDFRRVLELAPRLLEELLKMAVIPRTAQGHGWLRPAVGVVELSQCIVQAVPLSARKSSGVSSEGISPFLQLPHFSDAVVKKIARKKVKSFQDLQEMRLEDRSELLTQVAGLSATDVEDIEKVLEMMPSITVDITCETEGEEGIQEGDIVTLQAWVTLKRPNGLVGALPHAPYFPFHKEENYWVLLADSVSNNVWFSQKVSFLDEGGAITAASKAISESMEGSGAGVKETNDAVREAIEKVKGGSRLVMGKLQAPAEGTYNLTCFCLCDTWIGCDKKQAC"
  },
  {
    "reference": "MTMLPVIPTSPDTPDAVATRRRPAMPPRHLADLDLAGRRAAAAELGEPAFRAAQLSQHYFGRFERDVERMTDLPAKTRDALGERLMPRLLTSVKELSCDDGATRKSLWRLHDGAMVESVLMGYPDRITACVSSQAGCGMACPFCATGQAGLTRNLSVAEIVDQVVHLAGVAASGAVTGSPPRLSRIVFMGMGEPLANYPRVIEAVRRLTTPAPEGLGLSQRHITVSTVGLVPAMRRLIAEDLSVTLALSLHAPDDDLRDELVPVNQRWKVDEVLDVAFEYAARTGRRVSIEYALIRDVNDQPWRADLLGRRLAGKLAHVNLIPLNPTPGSRWDASPKPAEREFVRRLRAAGVATTVRDTRGREIDGACGQLAASELTDVGAVGGADGGRPAVPGAGAAEVAQ",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. [4Fe-4S] cluster is required for the proper folding and stability of the protein.2. The designed protein must have at least one Radical SAM core domain.3. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.4. The protein can be targeted to cytoplasm and perform rRNA (adenine-C2-)-methyltransferase activity, rRNA binding.5. The protein should have a high affinity for [4Fe-4S] cluster.6. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. The designed protein sequence is ",
    "response#1": "MNLLDLDRKGLEAFFAGLGEKPFRATQLMKWIYHEGVEGFDLMTNFSKTLRAYLSEHCSAQEKLTFCLSSQIGCKVGCVFCATAKGGFVRNLKAGEIVEQVVALKRMHSLEPTKGINLVFMGMGEPLHNFEQVVRSLKILSHPHGLNISPRRITLSTSGVVPMMDILGALNLGVQLAISLHAVNDELRSKLMPINKTYNIQELIKAARRFPIDARKRLMFEYLVIKDYNDGLEHAKALLRLLNGLRSKINLIPYNPTTHSKFERPDLEKVKQFADFLNQRGLLCTMRLSKGLDASAATTSDE",
    "response#2": "MNLLDLDRKGLEAFFAGLGEKPFRATQLMKWIYHEGVEGFDLMTNFSKTLRAYLSEHCAVSTQVGCTLDCGFCVTGQMGLKRNLKAHEIVDQVLTAQDQLRSGDRISSLVFMGMGEPLANFDELSEALTRLTDTEWGVGFPKRRITISTAGFSPRFHDVARLGVNLAVSLNATTSEQRDRLMPMMNRLHPLPDLLDACRAYPIPANRHLTFEYVLLQGENDSAARMTRAGDFYKEEDRGTLCISSQVGCTLNCTFCHTGTQTLVRNLTTAEIVGQILVCRDDLGEWTSAGKQVGEKRLLSNIVLMGMGEPLYNTDNVRDAMRIAMDDAGLGLSRRRITLSTSGVVPEIVRAGEEIGCMLAISFHATRDELRNELVPINRKWNIAALLEACRNWPRLSNAERITFEYVMLKDVNDSDADARRLVKLIAGIPAKINLIPFNPWPGSPYERSDWDRIEAFAEIVNRAGYASPVRTPRGQDVAAACGQLRAELL",
    "response#3": "MNLLDLDRKGLEAFFAGLGEKPFRATQLMKWIYHEGVEGFDLMTNFSKTLRAYLSEHCSIGSLETVEQKISRDGTIKNLLRLHDGHAIESVLIPAPAYRGKQLLDWLYRKFVFEPERMTNLPAGFREHLSIEDGERLTQCVSTQVGCTLDCGFCVTGQMGLKRNLKAHEIVDQVLTAQDQLRSGDRISSLVFMGMGEPLANFDELSEALTRLTDTEWGVGFPKRRITISTAGFSPRFHDVARLGVNLAVSLNATTSEQRDRLMPMMNRLHPLPDLLDACRAYPIPANRHLTFEYVLLQGENDSAADAVRLSRLLHGIRCKINLIPFNEFPDSPFRRPADPVIAAFQTILRQKGHDVFVRQNRGRDIDGACGQLRRG"
  },
  {
    "reference": "MELDIKAIMERLPHRYPMLLVDRVLDIVPGKSIVAIKNVSINEPFFEGHFPHHPVMPGVLITEAMAQAAALFSFTDDDGGLKCDSAKTAYYLVGIDEARFRRPVVPGDQLRLEVQAERLSRAICKYQARALVDGQIVAEAKLMCAIRSLEA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein with 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity, 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity functions is required to enable fatty acid biosynthetic process.2. The protein's active site must be carefully engineered to ensure that the amino acids involved in a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O are properly positioned for optimal catalysis.3. The protein must exhibit the following characteristics: Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.; Involved in unsaturated fatty acids biosynthesis. The designed protein sequence is ",
    "response#1": "MAEAVKTGQERVERALESIAAGDMIVVVDDEDRENEGDLVMAAEKVTPEAINFMITHGRGLVCLPMTGERLDALDLPMMVDNNTSPLGTNFTVSVDARSGVTTGISAADRARTIQVLVDPSTKPEDLVRPGHIFPLRARPGGVLVRTGQTEGSVDLARLAGLKPAGVICEIMNDDGTMAREVVDFCKRHQIKIVTIADLVRYRMRHERLVRC",
    "response#2": "MENTVNSTDPAVAGAADVSTPGASHRSPWPYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGIGGFGALCALPQKYREPVSVAIGGLGAPVVVDGQGRALLGENIGYSAIGQAMDLTARGWLQHRPMTMVGGTSNVSDQTLMKVVEGVEAAGYQSAIVLTQAVYHQARAAGLRPALLGTDSVALTGGAVAA",
    "response#3": "MIFDLIRDRNRNRVLNVMDAAELARVLKCPSICIGNFVQRADGSIIAAAGTPECTTPCGKMKCVMKDAKECPGEMPMSCGKASCEVMTVEKNIPGMSVPLTCKGCPVRAVTVTEPSHETDYGC"
  },
  {
    "reference": "MDEKPTTRKGSGFCSLRYALALIMHFSNFTMITQRVSLSIAIIAMVNSTQHQDPANASTEGPVMDLLSNQSRGIKDFSTRAAVYQWSTETQGIIFSSISYGIILTLIPSGYLAGIFGAKQILGAGLLISSLLTLFTPLAADFGVILVIVIRTVQGMAQGMAWTGQFTIWAKWAPPLERSKLTSIAGSGAAFGSFIILCVGGLISQALGWPFIFYIFGSIGCVCCVLWFTVIYDDPMHHPCISVREKEHITSSVAQQSSSPRRSVPIKAMVRCLPLWAIFMGFFSHFWLCTIIITYLPTYISTVLHVNIRDSGVLSSLPFIAASSCTILGGQMADFLLSRNLLSLITVRKLFSSLGLLLPSLCAVALPFVTSSYIATIVLLILIPGTSNLCDSGFIINTLDVAPRYASFLMGISRGFGLTAGIISSTTTGFLISQDSESGWRNVFFLSAAVNMFGLIFYLIFGQAEIQSWAKERTLTRL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have a specific function within the plasma membrane, membrane. The designed protein sequence is ",
    "response#1": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPV",
    "response#2": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPR",
    "response#3": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPI"
  },
  {
    "reference": "MLSIIGFIITISILVFIHEFGHYCIARYFNVKVEEFSIGFGKALIGITDKKGVRWKICLIPLGGYVKIYGYDRSLMDKTKEVNEKVAFDAKSCLERFLIVAAGPLINYLLAIIIFAGFYCYFGKTEIPPIIGNVVASSPAERADLRAGDKIVKVNDKSVKDFGDVQREILINGFSSSTLTIERKSEEFIVNIMPQEIIISPPEEKQVNKKTLRIGIIAKNESIHTKIGILGGLWEAINTTIDMSALTLNAISQMIVGKRSFDEIGGPIAIAKESGKSIAGGTQMYLLFIAMLSVNLGLLNLLPIPVLDGGHLVFILYEAITGKLPHPKTKNILLQLGAIIIIFLIIIAVSNDIQNLFS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization.2. The presence of PDZ domains is critical for the interaction of the designed protein with its intended targets.3. The protein requires Zn(2+) to activate a critical catalytic site.4. The designed protein should be involved in proteolysis by localizing to plasma membrane and exhibiting metal ion binding.5. The designed protein must have a active site that is structurally stable and resistant to denaturation.6. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding. The designed protein sequence is ",
    "response#1": "QEEVWLVETVRRLSTKAGFAMPEVAIYDGAPNAFATGPSKSRSLVAVSTGLLHSMDRKQVEAVLAHEVSHINNGDMVTLTLIQGVLNTFVIFLSRIIAYAVDSFLRRDEDESGSPGIGYWISSIACEILFGILASIVVMFFSRKREYRADAGAAALMGDRRPMIDALRALGNLDAGQLPKEMAASGIAGGGMMALFSSHPPLESRIAALEE",
    "response#2": "MPKGVILYGSPGTGKTLLARALASEAGVEFLAVSGSDFVQVYAGLGAGRIRNLFKKAKDKGKCVIFIDEIDAIGKKRDRGGLGGSDESDRTLNALLTEMSGFKGSEGIIVMAATNRLDILDDALLRPGRFDRQIEIGLPGRDHAELIRRNLDLHKLAEQTPGFAGADIANVCNEAALIAARKGKASVEMIDFEWLRGIAQQTVYFSGAKLENLMNEAAIYAAREEADFITEGHIDHIVEDMAMIAQIPMPRVFIVEDDSLNAFATGSKPENAAVAATTGLLAVMNREELEGVIGHEVSHIRNYDIRISTIAVALASAVTLISSIGSRMLFYGGGRRRDDDREDGGNILVLIFSILSLILAPLAASLVQLAISRQREYLADASSVELTRNPQGMISALEKLDRSEPMGHPVDDASAALYINDPTKKEGLKSLFYTHPPIADRIERLRELLG",
    "response#3": "QEEVWLVETVRRLSTKAGFAMPEVAIYDGAPNAFATGPSKSRSLVAVSTGLLHSMDRKQVEAVLAHEVSHINNGDMVTLTLIQGVLNTFVIFLSRIIAYAVDSFLRRDEDESGSPGIGYWISSIACEILFGILASIVVMFFSRKREYRADAGAAALMGDRRPMIDALRALGNLDAGQLPKEMAASGIAGGGMMALFSSHPPLESRIAALEAT"
  },
  {
    "reference": "MKFTALAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEALYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFFVQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITLKELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQIKDIEVNLK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be expressed specifically in the extracellular region2. For general function, the protein need meet that Plays a role in the inhibition of host complement activation via the classical pathway by interacting with the Fc region of human IgG and thereby interfering with the IgG/C1q interaction; Does not affect the protease activity of thrombin but interferes with the conversion of prothrombin to thrombin; Inhibits also the penultimate step of plasma clotting by interacting with prothrombin/F2 and coagulation factor X/F123. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "IVNGTLTKQGDSPWQAILLDSKKKLACGGVLIHTSWVLTAAHCVEGTKKLTVRLGEYDLRRRDHWELDLDIKEILVHPNYTRSSSDNDIALLRLAQPATLSKTIVPICLPNNGLAQELTQAGQETVVTGWGYQSDRIKDGRRNRTFILTFIRIPLVARNECVEVMKNVVSENMLCAGIIGDTRDACDGDSGGPMVVFFRGTWFLVGLVSWGEGCGHTNNYGIYTKVGSYLKWITASHW",
    "response#2": "LCGMVWEHKKGNDYHKQPWQAKISVTRPLKGHETCMGAVVSEYFVLTAAHCFMVDDQKHSIKVSVGGQRRDLEIEEVLFHPKYNINGKKAEGIPEFYDYDVALVKLKNKLKYGQTLRPICLPCTEGTTRALRLPQTATCKQHKEQLLPVKDVKALFVSEQGKSLTRKEVYIKNGDKKASCERDATKAQGYEKVKDASEVVTPRFLCTGGVDPYADPNTCKGDSGGPLIVHKRSRFIQVGVISWGVVDVCRDQRRQQLVPSYARDFHINLFQVLPWLKDKLQ",
    "response#3": "PRQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERSIEKISMLEKIYIHPRYNWRENLDRDIALLKLKKPIIFSDYIHPVCLPDKETAIRLLRAGYKGRVTGWGNLKEMWTSSVTEVQPSVLQVVNLPIVERPVCRASTRIRITDNMFCAGFKPNEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKTGS"
  },
  {
    "reference": "MQVVTRDEMYAVDRYAGEEIGLTGLLLMENAGQAAAKKIMERLTGSEQLAVLIGTGNNGGDGFVLARVLKSMGYGVDVWLIPEKNKIKEDAKKAMIIYERSGFSYRSYHQEGHECFLSSLANTEVIVDALLGIGLKESVRSPYANIIDAVNSQSAFILSIDLPSGVPADGGPVSLAIQADETITLQCPKVGAYTFPSADYYGKLTVVDIGIPPKAFQQLPVRKVWQVMDVKTALPVRKPSSHKGTYGKGLVVGGSLSMPGAPIMTAKAALRSGSGLLTVAVPEEIHSIAASHISEAMFTPFSNQTSLFQKPDEQIPYDTIAIGPGLGRDSYAEGRVREALTLSVPLVIDADGLFHLKTMLPLLKKRQAPTILTPHSGEMARLLEWSVEEVEHNRIQLSKQFAKEYGLYLVLKGPYTIVTTPTGSQFINTTGNAALAKGGSGDVLTGMILAFLMQHKVIQEALSNAVYLHGAAADYLVKTSHSKLDVLASDLIEVLPILLKKYTR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have at least one YjeF N-terminal domain.2. The protein design should be able to enhance the efficiency of nicotinamide nucleotide metabolic process.3. The designed protein should have a high specificity for (6S)-NADPHX binding, with minimal off-target effects.4. The protein must bind to Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "PRESNTHKGTYGHVLVVAGTLQMSGAGLLCSKTALRAGCGLVTWTLPRMVANQMLGKLPEVMLVPVEDKNNGEWNVHSAEDIIQLSENKQVLVIGPGLGRFEDDEMWLHKLWERIECPIVLDADALNILSDAEHFDKWSGKDTPVIITPHPGEMARLLKCTVKEVQENRIQIAREYAMKHKIVVVLKGAHTVIATANENVYINHTGNAGMATGGSGDVLAGMIGGLLAQNYTAEQAAVLGVYLHGLAGDRAAAKRYSQHSLIAGDIIDEI",
    "response#2": "GHKYDHGHAVVLSGPMGRSGAARLAARAALRIGAGLVTVAAPGAAMLECAAQLTAIMLRRCDGTDGLGALLVDDRVTALCLGPGLGVGPGTEDLVEVALTAPGADGRGVVLDADALTSFEGAPGRLFALTSRVDRVVLTPHVGEFRRLFPDLAERLDAAPVRGPAYSRLDAVRAAAGRAGCAVVLKGPDTVIGGPGGAAAVHGAVYDRSVPWLATAGAGDVLAGLIAGLLARGQGAMPAAQAAAWLHVEAARRVGPGLVAEDLAEALPA",
    "response#3": "HKYDHGHAVVLSGPMGRSGAARLAARAALRIGAGLVTVAAPGAAMLECAAQLTAIMLRRCDGTDGLGALLVDDRVTALCLGPGLGVGPGTEDLVEVALTAPGADGRGVVLDADALTSFEGAPGRLFALTSRVDRVVLTPHVGEFRRLFPDLAERLDAAPVRGPAYSRLDAVRAAAGRAGCAVVLKGPDTVIGGPGGAAAVHGAVYDRSVPWLATAGAGDVLAGLIAGLLARGQGAMPAAQAAAWLHVEAARRVGPGLVAEDLAEALPGLLA"
  },
  {
    "reference": "MKIDILTLFPEMFAPLEHSIVGKAREKGLLEINYHNFRENAEKSRHVDDEPYGGGQGMLLRAQPIFDAYDDIEKKQPRVILLDPAGRTFDQAYAEELAKEEELIFICGHYEGYDERIKTLVTDEISLGDYILTGGELAAMTMIDATVRLIPEVIGKEASHTDDSFSSGLLEYPQYTRPYDYRGMVVPEVLMSGHHENIRKWRLYESLKKTYLRRPDLLKHYEMTVEEEAMLEEIRHAHSD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein localizes to the cytoplasm. The designed protein sequence is ",
    "response#1": "CCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGK",
    "response#2": "CCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGK",
    "response#3": "CCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGK"
  },
  {
    "reference": "MDSKKKSSAEAEGSKERGLVHVWQAGSFSLTPERLPGWGGKTVLQAALGVKHGVLLTEDGEVYSFGTLPWKSEAAEICPSSPLLESALVGHHVVTVATGSFHSGAVTESGVVYMWGENAAGQCAVANQQYVSEPSPVSISDSETSPLLAVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPPQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEKHASTVTSPHPETLDGQGEVFENTVAEAELNMGSDQTTSGSAISAQQNIVGMAEVSSARTAPSYPDTQTVTAYLQKLSEHSVKENHEREEKLPQVQPLVEEAVPDLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTAPCEPGAPSGTASTGPESLKDLREEQVKQESLQGKKSSSLMDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGSHSLALTAKSQVYSWGSNTFGQLGHSEFPTTVPRLSKVSSESGVWSVAAGHGYSLFLVDTEDFQPGLYYSGRQDRAEGDTLPENPSGTTTPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELATTERRFYSKLSEIKSQILRPLLSLEHLGTVTTVQLLQEVASRFSKLCYLIGQHGASLSSYLQGMKEARNLVIMKH",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have identical protein binding, GTPase activator activity, small GTPase binding within axon, nucleus to regulate axonogenesis, endosomal transport, protein localization, positive regulation of GTPase activity.2. The presence of DH, VPS9, PH domains is critical for the interaction of the designed protein with its intended targets.3. The protein must exhibit the following characteristics: May act as a GTPase regulator . The designed protein sequence is ",
    "response#1": "KPLEEHLTISGREIAFPIEACVTMLLECGMQEEGLFRVAPSASKLKKLKAALDCCVVDVQEYSADPHAIAGALKSYLRELPEPLMTFELYDEWIQASNVQEQDKKLQALWNACEKLPKANHNNIRYLIKFLSKLSEYQDVNKMTPSNMAIVLGPNLLWPQAEGNITEMMTTVSLQIVGIIEPIIQHADWFR",
    "response#2": "KPLEEHLTISGREIAFPIEACVTMLLECGMQEEGLFRVAPSASKLKKLKAALDCCVVDVQEYSADPHAIAGALKSYLRELPEPLMTFELYDEWIQASNVQEQDKKLQALWNACEKLPKANHNNIRYLIKFLSKLSEYQDVNKMTPSNMAIVLGPNLLWPQAEGNITEMMTTVSLQIVGIIEPIIQHADWFS",
    "response#3": "PKLYSWDQREKVDPKDYMFSGLKDETVGRLPGKVAGQQFVIQDCENCNIYIFDHSATITIDDCTNCVIFLGPVKGSVFFRNCRDCKCTLACQQFRVRDCRKLEVFLCCATQPIIESSTNIKFGCFQWYYPELAAQFKDAGLSIFNNIWSHVHDFTC"
  },
  {
    "reference": "MHQTNFGFKKVDYTEKQRLVNNVFSNVADKYDLMNDLMSFGLHRLWKDEFIRYIPNLNSHILDVASGSGDIALKLAKKARDRGNNVSLTLSDINEEMLRNAKKKAIDLNLFQNLKFTLASAAALPFSDNSFDYYTIAFGIRNVPDINKALKEAYRVLKPMGKFICLEFSKVKEGILKDFYKFYSFNIIPQIGQMIAGNKEAYEYLIESIDLFPSQDKFRIMIKEAGFEEVNYKNLSNGIVAVHSAYKI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis pathway to avoid interference with native metabolic processes.2. The protein should have a high affinity for S-adenosyl-L-methionine.3. We need a protein that can perform a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine with high efficiency.4. The designed protein should have S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity to facilitate menaquinone biosynthetic process, methylation, aerobic respiration. The designed protein sequence is ",
    "response#1": "MTETPRPDARTDDHPAPARPADAAAPRHGDDTVVRPGGPARDAAEAVLARVGRGSVIVLADDEDRENEGDLTLAAQFATPEVINFMTREARGLICVPLTEERAAALDLPPMVARNTDTHATAFTVSVDFNQTTTGISAHDRATTIRALVDPNATPADFRRPGHVFPLRAREGGVLRRPGHTEAAVDLARLAGLTPAGVLVEVVNEDGTMARLPRLREFAEDHDLKLISIADLIAYRRRTEKLVRRETTAAAEAIPAGDGAAGEAGAAPVRAPGPDLDGPAGAPAPRPAPDHRHGPVGPAPGPRAAAGADDGADDGDADADAGGDRPAAPAPAGADLGDPDAGPADPLTPGDDPAAYLATKRERMGHLLEPLPEGEDAA",
    "response#2": "MNNYYQILGIPSTATQQEIKSAYYALSKQYHPDHNPNNPNSAHQFLQISKAYEVLGNHSLRASYDRELALEKQKQQAAEKQKAQQQAQQKAHQKAHQKAAQERQQREQAAQAQQKAQRKAQQQAHQKAHQKAHQRAQQAPPRKATAGAQTANSKSLPHETVLNFMEKAKSAYKKKDYNTAISYCNRALLLDPKNARYHSLRGEAYRMKNDRKGALSDYKKAASLGNKL",
    "response#3": "MSVTLKKKVLIGTCGNPDLGDDALGFAIIEELRRRYDFVTLDAGEMTIPGLKGYDVVAFLHSTYGGGCRPDFAIVIVDEPDTRRAWEGREYTTALAKGIEGELTVAGGACPHFCIIEMPMSTAIKMANQIDPSITLNFGTRSKYPELQLCGECGMEKILEPRLVEALKVWEKAYGGTPNLRL"
  },
  {
    "reference": "MKRTPLYERHVALGAKMVDFAGWIMPLYYSSIFEEVMAVRKSVGVFDVSHMGEIVVEGQETVDFVNFLVTNDFSAIPEGKAMYTVMCNETGGIVDDLVVYRISHEKAIMVVNAANIEKDYEWIKVHAKNFNVEVRNVSDETALVAFQGPKSQETLQRVVDIDLEGIGYYSFQWGRLDGERVLVSRTGYTGEDGFELMMNAESAAKIWDTLVEIAGNVDGKPAGLGARDVCRLEASYLLYGQDMDESTNPFEVGLSWVVKMNKDFVGKEALLKLKEKVERKLVALELSGRRIARKGYTVLKEGKEVGKITSGNFSPTLGKSIALALVSRCVKTGDRLEVVFPGKNVEAHVVKKPFYRGSVRREA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. We are looking for a protein with broad substrate specificity for (6S)-5,6,7,8-tetrahydrofolate + (R)-N(6)-(S(8)-   aminomethyldihydrolipoyl)-L-lysyl-[protein] = (6R)-5,10-methylene-   5,6,7,8-tetrahydrofolate + (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] +   NH4(+). The designed protein sequence is ",
    "response#1": "LQEVDAEVFNCISGELNRQNSGLQLIASENFVSKAVLQAQGSIFTNKYAEGYPGKRYYCGCHFADIVENLAIERLCRLFGCKFANVQPHSGSQANQGVFAALLKPGDTVIGMSLDCGGHLTHGSAPSISGKWFNAVQYQVDRDTGLIDMDEIEKLAVEHNPSLIIAGSSSYPRVIDFKRFREIADKVGAYLLADIAHYAGLIAAGEFPSPVEYAHVITSTTHKTLRGPRGAVIMTNYEDIHKKIQSSIFPGMQGGPLMHVIAAKAVAFAEALKPEFKDYAKQIIKNSKALGEVFKERGLDLVTGGTDSHMVVLDLRSKSVTGKDAVLALEKLGIICNKNAIPFDPEKPFVTSGLRFGSAAETSRGLQESEFREIGSMIAKGIEAVAE",
    "response#2": "LQEVDAEVFNCISGELNRQNSGLQLIASENFVSKAVLQAQGSIFTNKYAEGYPGKRYYCGCHFADIVENLAIERLCRLFGCKFANVQPHSGSQANQGVFAALLKPGDTVIGMSLDCGGHLTHGSAPSISGKWFNAVQYQVDRDTGLIDMDEIEKLAVEHNPSLIIAGSSSYPRVIDFKRFREIADKVGAYLLADIAHYAGLIAAGEFPSPVEYAHVITSTTHKTLRGPRGAVIMTNYEDIHKKIQSSIFPGMQGGPLMHVIAAKAVAFAEALKPEFKDYAKQIIKNSKALGEVFKERGLDLVTGGTDSHMVVLDLRSKSVTGKDAVLALEKLGIICNKNAIPFDPEKPFVTSGLRFGSAAETSRGLQESEFREIGSMICEDVLKGME",
    "response#3": "LQEVDAEVFNCISGELNRQNSGLQLIASENFVSKAVLQAQGSIFTNKYAEGYPGKRYYCGCHFADIVENLAIERLCRLFGCKFANVQPHSGSQANQGVFAALLKPGDTVIGMSLDCGGHLTHGSAPSISGKWFNAVQYQVDRDTGLIDMDEIEKLAVEHNPSLIIAGSSSYPRVIDFKRFREIADKVGAYLLADIAHYAGLIAAGEFPSPVEYAHVITSTTHKTLRGPRGAVIMTNYEDIHKKIQSSIFPGMQGGPLMHVIAAKAVAFAEALKPEFKDYAKQIIKNSKALGEVFKERGLDLVTGGTDSHMVVLDLRSKSVTGKDAVLALEKLGIICNKNAIPFDPEKPFVTSGLRFGSAAETSRGLQESEFREIGSMIAKTLIEGGDK"
  },
  {
    "reference": "MSVILDDDVLLILTLDEELSAPLTPSNGLGQEDLPSKNGGGQSGPNSQVPSLVSGADSPPSSPPGHNWEMNYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPALKLRTYVHATLELFALMVVVFELCMKLRWLGFHTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFNTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYHLLVSQRRPAGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKAKKNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFISKHVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFSGRLSPNCCNSSTVADAYRFINHTVGNKTKVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKSQESEVDSGIVIEKEMSKEELLAILELHREARGTSSDVTRLLDTLSQMEKYQQNSMVFLGRRSRTKSD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The ideal protein would be one that plays a critical role in regulation of ion transmembrane transport, endocytosis involved in viral entry into host cell through ligand-gated sodium channel activity, protein homodimerization activity, syntaxin binding within endosome membrane, membrane, endolysosome, recycling endosome membrane.2. Design the protein with a signal peptide incorporated.3. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.4. The protein should contain Helical; Name=S1 of repeat I, Helical; Name=S2 of repeat I, Helical; Name=S3 of repeat I, Helical; Name=S3 of repeat II, Helical; Name=S6 of repeat I, Helical; Name=S5 of repeat II, Helical; Name=S5 of repeat I transmembrane regions to facilitate transport across the membrane.5. For general function, the protein need meet that Intracellular channel initially characterized as a non- selective Ca(2+)-permeable channel activated by NAADP (nicotinic acid adenine dinucleotide phosphate), it is also a voltage-gated highly- selective Na(+) channel activated directly by PI(3,5)P2 (phosphatidylinositol 3,5-bisphosphate) that senses pH changes and confers electrical excitability to organelles The designed protein sequence is ",
    "response#1": "KFVKVPSSVAPSVLFELLLTEWHLPAPNLVVSLVGEERPLAMKSWLRDVLRKGLVKAAQSTGAWILTSALHVGLARHVGQAVRDHSLASTSTKIRVVAIGMASLDRILHRQLLDGVHQKEDTPIHYPADEGNIQGPLCPLDSNLSHFILVESGALGSGNDGLTELQLSLEKHISQQRTGYGGTSCIQIPVLCLLVNGDPNTLERISRAVEQAAPWLILAGSGGIADVLAASALSQFGKAQTQETPLWLLLQVAPIRNERDLVVLFLLTFRDITALKQPIDSEDLVDFPIVYCNESFCKISGYNRAEVMQKSCRYVCGFMYGELTDKETVGRLEYTLENQQQDQFEILLYKKNNLQCGCALSQFGKAQTQETPLWLLLQVAPIRNERDLVVLFLLTFRDNNRPMLSLSKFVKVPSSVAPSVLFELLLTEWHLPAPNLVVSLVGEERPLAMKSWLRDVLRKGLVKAAQSTGAWILTSALHVGLARHVGQAVRDHSLASTSTKIRVVAIGMASLDRILHRQLLDGVHQKEDTPIHYPADEGNIQGPLCPLDSNLSHFILVESGALGSGNDGLTELQLSLEKHISQQRTGYGGTSCIQIPVLCLLVNGDPNTLERISRAVEQAAPWLILAGSGGIADVLAASCGNFDDEMSEEELFRGMDDLILDNICDRAKPRVFSKDEKIIREGDPVQRMIFIMRGRVKRIQSLSKGVLATSTLEPGGYLGDELLSWCLRRPFLDRLPPSSATFVCLENIEAFSLGSEDLRYITDHFRYKFANERLKRTAR",
    "response#2": "MCLKIIKEYERAVVFRLGRIQADKAKGPGLILVLPCIDVFVKVDLRTVTCNIPPQEILTRDSVTTQVDGVVYYRIYSAVSAVANVNDVHQATFLLAQTTLRNVLGTQTLSQILSGREEIAHSIQTLLDDATELWGIRVARVEIKDVRIPVQLQRSMAAELDTHRGQQECKFVKVPSSVAPSVLFELLLTEWHLPAPNLVVSLVGEERPLAMKSWLRDVLRKGLVKAAQSTGAWILTSALHVGLARHVGQAVRDHSLASTSTKIRVVAIGMASLDRILHRQLLDGVHQKEDTPIHYPADEGNIQGPLCPLDSNLSHFILVESGALGSGNDGLTELQLSLEKHISQQRTGYGGTSCIQIPVLCLLVNGDPNTLERISRAVEQAAPWLILAGSGGIADVLAAFPPPTVLPSREAPPKFVKVPSSVAPSVLFELLLTEWHLPAPNLVVSLVGEERPLAMKSWLRDVLRKGLVKAAQSTGAWILTSALHVGLARHVGQAVRDHSLASTSTKIRVVAIGMASLDRILHRQLLDGVHQKEDTPIHYPADEGNIQGPLCPLDSNLSHFILVESGALGSGNDGLTELQLSLEKHISQQRTGYGGTSCIQIPVLCLLVNGDPNTLERISRAVEQAAPWLILAGSGGIADVLAAFPPPTVLPSREAPPPREAPPAREAPPAREAPPAREAPPAREAPPAREAPPAREAPPAREAPPAREAPPAREAPPAREAPPAREAPPAREAPPAREAPPAREAPPAREAPPAREAPPAREAPP",
    "response#3": "MCLKIIKEYERAVVFRLGRIQADKAKGPGLILVLPCIDVFVKVDLRTVTCNIPPQEILTRDSVTTQVDGVVYYRIYSAVSAVANVNDVHQATFLLAQTTLRNVLGTQTLSQILSGREEIAHSIQTLLDDATELWGIRVARVEIKDVRIPVQLQRSMAAEAEQGSHPTEKRKFVKVPSSVAPSVLFELLLTEWHLPAPNLVVSLVGEERPLAMKSWLRDVLRKGLVKAAQSTGAWILTSALHVGLARHVGQAVRDHSLASTSTKIRVVAIGMASLDRILHRQLLDGVHQKEDTPIHYPADEGNIQGPLCPLDSNLSHFILVESGALGSGNDGLTELQLSLEKHISQQRTGYGGTSCIQIPVLCLLVNGDPNTLERISRAVEQAAPWLILAGSGGIADVLAAVDFPIVYCNESFCKISGYNRAEVMQKSCRYVCGFMYGELTDKETVGRLEYTLENQQQDQFEILLYKKNNLQCGCALSQFGKAQTQETPLWLLLQVAPIRNERDLVVLFLLTFRDITRENVLQRDFPIVYCNESFCKISGYNRAEVMQKSCRYVCGFMYGELTDKETVGRLEYTLENQQQDQFEILLYKKNNLQCGCALSQFGKAQTQETPLWLLLQVAPIRNERDLVVLFLLTFRDGQRSPRAWIGLDNHRYFDYLGNLIALGNLLSICVFLVLDSNLLPGERNDFVLGILNYIFILYYLLELLFKVFALGLRGYLFYHSNVFDGLLTIILLVLEISTLAVYRLPHPGWKPEQYGPLSLWDMTRLVNILIVIRFLRIIPSIKPTAVVASTILGLIQNLRAFGGILVVVYYVFAMIGINLFRGVIVPPGNSSLVPDNSSAPCGSFEQLGYWPNNFDDFAAALITLWDVMVVNNWQVILEAYKHYSGPWSMVYFVLWWLVSSVIWINLFLALLLEN"
  },
  {
    "reference": "MIKKVHHALIENELTKLRDKSSSSSQFRMALSQITSLLFFEVTKESPLEEIVVKTPFTKTKGYKLKNEIVLVPIMRAGLGMVDPIVRYSEKIRVGHLGIYREHEGTNVISYYKKMPENIGNSHVIILDPMLATGTTLATAISSIKKNHPIKISVVAIVAAPEGIKAVEAAHPDVDIYLAAIDEKLNKDNYIIPGLGDAGDRLFGTK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein with GTP binding, magnesium ion binding, uracil phosphoribosyltransferase activity functions is required to enable uracil salvage, UMP salvage.2. For general function, the protein need meet that Catalyzes the conversion of uracil and 5-phospho-alpha-D- ribose 1-diphosphate (PRPP) to UMP and diphosphate.3. We are looking for a protein with broad substrate specificity for diphosphate + UMP = 5-phospho-alpha-D-ribose 1-diphosphate +   uracil.4. The protein design should consider the endogenous proteins and enzymes present in the UMP biosynthesis via salvage pathway, Pyrimidine metabolism, UMP from uracil: step 1/1 pathway to avoid interference with native metabolic processes.5. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.6. The uracil, 5-phospho-alpha-D-ribose 1-diphosphate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "VFDHPLIQHKISLLRDKNTNTKEFRELVSEIAMLMGYEVTRNMPLKEVEIETPIGMAKTKVISGKKLGIVPILRAGLGMVDGMLRLIPMAKVGHIGLYRDPESLQPVEYYCKLPVDVEEREIVVLDPMLATGGSASAAIQFIKNRGVKNIKLMCLIAAKAGIERMQKDHPDVGIYCASVDEILNDHAYIVPGLGDAGDRLFGTK",
    "response#2": "VLNHPLIQHKLTIIRRKETGSNEFRRIVGEIAGLMTYEITRDLPLQDVEIETPMGKTVQKELAGKKLTIVPILRAGMGMVNGVLEMIPSAKVGVVGMYRDEETLKPVEYFFKVPKDVTERECLVVDPMLATGGSANLAIEALKKRGVTDIKLAVLVAAPEGVKAVQDEHPDVDIYAAALDDKLLPNGYIFPGLGDAGDRIFGRVE",
    "response#3": "VFDHPLIQHKISLLRDKNTNTKEFRELVSEIAMLMGYEVTRNMPLKEVEIETPIGMAKTKVISGKKLGIVPILRAGLGMVDGMLRLIPMAKVGHIGLYRDPESLQPVEYYCKLPVDVEEREIVVLDPMLATGGSASAAIQFIKNRGVKNIKLMCLIAAKAGIERMQKDHPDVGIYCASVDEILNDHAYIVPGLGDAGDRLFGKLD"
  },
  {
    "reference": "MLSEQTLSLVKATVPVLQQHGVTLTSHFYRRMFEHNPELKNLFNQGHQHSGQQQQALAGAVLAYAQHIEDPSPLLPVLIRVAHKHVSLGIRAEHYPIVGKHLLASIREVLGEAASDDLIQAWAEAYGLLADTLIGIENGMYGESTNAAGGWSGWRPFRVARKVAESEEIASFYLEASDGGALPAFKPGQYVSVKRFVGEWGLSQPRQYSLSDAPNGEYLRISVKREDAAEGKPAGRVSNLLHREVSEGDVLELTAPQGDFFLHEDRSGPAVLISAGVGQTPMQAMLGRLLKQGGREVRFLHAARHGGAHAMGLKVRQLAERHPQLQIHVRYETPRPQDVMGVDYHAAGRLELDEVKALAVLPDADYYLCGPLGFMRAQRDSLRRLDVPAERIHYEVFGSNPGDD",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds2. A protein with FAD binding functions is required to enable response to toxic substance.3. The designed protein should have a high specificity for NADP(+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MLSTNTIQIVKSTAPVLAVKGEEITTHFYKTLFTNHPELLNIFNHANQKKGRQQTALANTIYAAATYIDQLEVLLPAVKQIAHKHRSLAVKLEHYPIVGEHLLEAIKAVLGDAATDEIIEAWREAYGVIADVFISVER",
    "response#2": "MLSENTIQIVKSTAPLLAKTGPQLTAHFYQRMFTHHPELKEIFNMSNQRNGDQREALFNAICAYANNIDNLPALLGAVEKIAHKHSSFLISAEQYQIVGSHLLATIDELFSPGEAVLDAWAEAYGVLANVFIARER",
    "response#3": "MLSTNTIQIVKSTAPVLAVKGEEITTHFYKTLFTNHPELLNIFNHANQKKGRQQTALANTIYAAATYIDQLEVLLPAVKQIAHKHRSLAVKLEHYPIVGEHLLEAIKAVLGDAATDEIIEAWREAYGVIADVFISVEV"
  },
  {
    "reference": "MDKMSTNMRDYRFWVVLSVLGLVQMSEGLHCLDSQRPCENGGRCIDSRCMCRPGYIGPLCQHLDPCHRSPCHNGAACKSQVANGIPQYTCVCQRGFRGQDCSLIDACATSPCANGARCTNWNNHYNCSCPPGFQGKNCRNDIDECRKPGKCLNGGMCVNTHGSFLCECLPGYSGRTCEVLTQPCAPSQCLNGGTCHQTGDHTYECACLPGFSGLNCEVNVDDCPGHKCMNGGICVDGVNTYNCQCPPEWTGQYCAEDVNECLMQPNACHNGGTCFNTNGGHTCVCVNGWTGNDCSENIDDCATAVCFNGATCHDRVASFFCECPVGKTGLLCHLDDACVSNPCNEGAVCDTNPLNGRAICTCPAGFVGGACNQDMDECSIGANPCEHFGKCVNTEGSFQCQCGRGYTGPRCEIDINECLSMPCQNDATCLDRIGQFSCICMPGYAGTHCEVDIDECESNPCVNDGICRDLVNGFTCKCQPGFTGTMCQIDIDECASTPCQNGAKCIDRPNGYECRCAEGFEGRLCESNIDNCQPDPCHHGTCIDGIASYTCNCVPGYTGYRCENQLNECHSNPCQNGGKCVDLVNKYICQCQHGTSGTNCEINFDDCASGPCDYGICKDGINRYDCVCKPGFTGPKCNVEVDECSSNPCRNGGTCVDEENGFHCLCPAGFHEPYCYSHVDVCSNSPCVHGNCREDPNGYRCDCEPGWTGKNCDLDRNDCVPSPCQNAGTCIDQLNGFTCKCHQGFRGNLCQENINECASSPCLNHGTC",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should emphasize Polar residues compositional bias.2. The designed protein must be able to regulate dorsal aorta development, maintenance of blood-brain barrier.3. The Ca(2+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "GILNLNKMIKQVTGKTPIFSYWPYGCHCGPGGKGQPKDATDWCCRKHDCCYAHLRQDRCRVHTDHYDYTFSHGDIQCSNKMNWCEQQLCACDKAVALCLKQNLDTYQRHLFYYWWPRC",
    "response#2": "NTIVHLFEWHWDDIANECENFLGPKGFAGVQISPPAENTVIGDRPWWERYQPISYALNTRSGDESALASMIRRCNNAGVRIYVDAVFNHMSATSGIGTGGSSCDVEPSASPAVPYGSGDFHGRCTSNNYQDPNNIRNCWLSGLPDLDQSKDYVRDKILEYLNHLVDLGVAGFRVDAAKHMWPADLQVIYGRVKDLNTDHGFSQGSRPFFYQEVIDLGGEGVSKNEYTGFGTVLEFKYGTELGNAFQGNNALHNLENWGPAWGLLEGTDAVVFIDNHDNQRTGSGAILTYKNPRPYKMAIGFMLAHPYGTTRIMSSFSFDYNDQGPPTQGPGFNSVRNLHQWVGGANTGWRQILRVMVGFR",
    "response#3": "PGKPEIFKCRSPNKETFTCWWRPGTDGGLPTNYSLTYHREGETLMHECPDYITGGPNSCHFGKQYTSMWRTYIMMVNATNQMGSC"
  },
  {
    "reference": "MKLIFATNNQHKVEEIQAAIGNDLEIISLKQAGIDIDIPEPHDTLEANASEKSRTIHQLTGQHCFSEDTGLEVEALNGEPGVKSARYAGEDKTFDKNIDKLLTRLGNGENRKARFRTVISLIWQGKEWLFEGICEGTIAKERHGAGGFGYDPVFMPAGSNRSFAEMTMAEKNALSHRKKAADKLVLFLQQQVIETR",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must have high specificity towards ribonucleoside triphosphate phosphatase activity. The designed protein sequence is ",
    "response#1": "EHRWSS",
    "response#2": "EPH",
    "response#3": "GVVARM"
  },
  {
    "reference": "MTTDTPPPKPGLTYAEAGVDIDAGNTLVDRIKPAAKATSRPGVMSGLGGFGALFDLRAAGYADPVLVAATDGVGTKLRIAIDTGHVDTIGIDLVAMCVNDLVCQGAEPLLFLDYFATGKLDVAEAATIVEGIARGCATSGCALIGGETAEMPGMYAKGDFDLAGFAVGAMERGGALPANVAAGDMILGLASDGVHSNGYSLVRRIVERSGLGWGDPAPFEGRTLGAALLTPTRLYVQPALAAIRAGGVHGLAHVTGGGLTENLPRVLPEGLGIEINLGAWELPPVFRWLAAEGGLDEAELLKTFNAGIGMALIVAPDRAEALADLLAGAGERVAVIGHVTEGAGAVHYRGTLL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must have high specificity towards phosphoribosylformylglycinamidine cyclo-ligase activity. The designed protein sequence is ",
    "response#1": "KSFMDRHNIPTARWSSFTDPQDACRYIRGAEFPALVVKASGLAAGKGVVVARDKDEACQAVLDIMKDKAFGSAGETVVVEELLEGEEVSCLCFSDGVTVSPMPPVQDHKRLLDGDQGPNTGGMGAYCPTPQVSEEVLQEIQTSVLQKTVDGMRAEGTPYVGVLYAGLMLTTEGAKVLEFNCRFGDPECQVLMPLLESDLYEVLMKTLASKSFSKSFMDRHNIPTARWSSFTDPQDACRYIRGAEFPALVVKASGLAAGKGVVVARDKDEACQAVLDIMKDKAFGSAGETVVVEELLEGEEVSCLCFSDGVTVSPMPPVQDHKRLLDGDQGPNTGGMGAYCPTPQVSEEVLQEIQTSVLQKTVDGMRAEGTPYVGVLYAGLMLTTEGAKVLEFNCRFGDPEV",
    "response#2": "KSFMDRHNIPTARWSSFTDPQDACRYIRGAEFPALVVKASGLAAGKGVVVARDKDEACQAVLDIMKDKAFGSAGETVVVEELLEGEEVSCLCFSDGVTVSPMPPVQDHKRLLDGDQGPNTGGMGAYCPTPQVSEEVLQEIQTSVLQKTVDGMRAEGTPYVGVLYAGLMLTTEGAKVLEFNCRFGDPECQVLMPLLESDLYEVLMKTLASKSFSKSFMDRHNIPTARWSSFTDPQDACRYIRGAEFPALVVKASGLAAGKGVVVARDKDEACQAVLDIMKDKAFGSAGETVVVEELLEGEEVSCLCFSDGVTVSPMPPVQDHKRLLDGDQGPNTGGMGAYCPTPQVSEEVLQEIQTSVLQKTVDGMRAEGTPYVGVLYAGLMLTTEGAKVLEFNCRFGDPER",
    "response#3": "KSFMDRHNIPTARWSSFTDPQDACRYIRGAEFPALVVKASGLAAGKGVVVARDKDEACQAVLDIMKDKAFGSAGETVVVEELLEGEEVSCLCFSDGVTVSPMPPVQDHKRLLDGDQGPNTGGMGAYCPTPQVSEEVLQEIQTSVLQKTVDGMRAEGTPYVGVLYAGLMLTTEGAKVLEFNCRFGDPECQVLMPLLESDLYEVLMKTLASKSFSKSFMDRHNIPTARWSSFTDPQDACRYIRGAEFPALVVKASGLAAGKGVVVARDKDEACQAVLDIMKDKAFGSAGETVVVEELLEGEEVSCLCFSDGVTVSPMPPVQDHKRLLDGDQGPNTGGMGAYCPTPQVSEEVLQEIQTSVLQKTVDGMRAEGTPYVGVLYAGLMLTTEGAKVLEFNCRFGDPEL"
  },
  {
    "reference": "MAKTASSLVLPIIFLVMFALVEQNMGCMAVLGSCGVITDCSGECISRFGPQARGYCDRDGGSGTCVCVYPCPADKPHM",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Ensure the presence of a signal peptide within the protein.2. The designed protein must be able to regulate killing of cells of another organism. The designed protein sequence is ",
    "response#1": "GDGVEGSVMVIDHAKGLIIWSIPRAGECYLIGGVDKQLPDAQELLHYFRSAQGSADGEGVQSALDYVKAEDRPVTDLNLLAPEVREACQGKSVYWE",
    "response#2": "SVMVIDHAKGLIIWSIPRAGECYLIGGVDKQLPDAQELLHYFRSAQGSADGEGVQSALDYVKAEDRPVTDLNLLAPEVREACQGKSVYL",
    "response#3": "GDGVEGSVMVIDHAKGLIIWSIPRAGECYLIGGVDKQLPDAQELLHYFRSAQGSADGEGVQSALDYVKAEDRPVTDLNLLAPEVREACQGKSVYWS"
  },
  {
    "reference": "MSNRYAVILAAGQGTRMKSKLYKVLHPVCGKPMVQHVSDMLGRVGFEETVIVVGHGSDAVKAQLGEDMHYVLQSEQLGTGHAVLQADDLLKDKKGITVVLAGDTPLLTEETITALIEEHHSQQAKITVLTALADDPSGYGRVIRGEDGLVQRVVEHKDATDDERLVNEINTSTYCFDNELLFKALKEVGNDNAQGEYYLPDVIEILQKQGEIIAAYQSPEFSETYGVNDRVALANAETLMKQRINERWMRAGVTILDPANTYISANAIIGQDTVVHPGVSIQGNTTIGRDCIIGANSEIVNSFVSDGVEVRQSVIHDSTVAENVTIGPFAHIRPGSELGAEVRIGNFVEVKKSSLAKGAKAAHLSYVGDAEVGENVNLGCGTITVNYDGINKHKTTIEDGAFIGSNSNLIAPVHIGKDALVAAGSTITDDVPGKALSIARARQVNKDDYKKN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein have MobA-like NTP transferase domains with specific sequence motifs that are crucial for its activity.2. The designed protein should have glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity, magnesium ion binding within cytoplasm to regulate cell morphogenesis, lipid A biosynthetic process, regulation of cell shape, UDP-N-acetylglucosamine biosynthetic process, peptidoglycan biosynthetic process.3. The acetyl-CoA, UDP-N-acetyl-alpha-D-glucosamine binding site should be located in a region of the protein that is accessible to the ligand.4. The protein design should take into account the physiological conditions of the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, Nucleotide-sugar biosynthesis pathway.5. Mg(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "MKLHEICGRSLVGHVLAASRELDPEHLVVVVGHAREQVTAHLAEIDPGVRTAVQEEQNGTGHAVRMGLEQLGGAVDGTVVVVCGDTPLLTAATLRQLAETHGADGNAVTVLTAEVPDATGYGRIVRDATGAVTAIVEHKDASEEQRAVREINSGVFAFDGALLADALKQVRTDNSQGEEYLTDVLGILREAGHRVGASVAADHREIAGINNRVQLSEARRILNDRLLTEAMLAGVTVVDPATTWVHEICGRSLVGHVLAASRELDPEHLVVVVGHAREQVTAHLAEIDPGVRTAVQEEQNGTGHAVRMGLEQLGGAVDGTVVVVCGDTPLLTAATLRQLAETHGADGNAVTVLTAEVPDATGYGRIVRDATGAVTAIVEHKDASEEQRAVREINSGVFAFDGALLADALKQVRTDNSQGEEYLTDVLGILREAGHRVGASVAADHREIAGINNRVQLSEARRILNDRLLTEAMLAGVTVVDPATTWVRDTEEILGVNTRQQLAEAEQVLRRRINTRHMLAGVTLADPARFDLRGEVSCGRDVYIDVDVVLSGHVVMGEGCQIGSHCVLTNVTLGQNCLIKSHSVLEDCEMADACEIGPFARIRPGTVLASNCKIGNFVETKNAVFDEGSKANHLSYLGDVKVGKAVNIGAGTITCNYDGANKHQTVIEDGVFIGSDTQLVAPVTVGKNATIGAGSTIRKSVPAGGLTLTKSEQRVIEGWVARKRPGSPSAEAAERARAGQSADGSGE",
    "response#2": "VVILAAGMGKRMQSALPKVLHPLAGKPLLAHVLDTARSLATNRVCVIYGHGGAAVLELLGKQPQPVATAQQEQQLGTGHAVMQALPELDDNAPTLILYGDVPLTTAASLQALIDAAGTDKLGILTVVQDPAELGRIVRGGGRVARGPEDRDITEVQAIVEGKSLNALRREIGAAQNDGLREVYLTDIVAAYRNAGLSIREGVTLVDPASTFVDAQVEIGRDTVIMPFTVISGSTSIGQSCNIGPYVQIRDSSVGDRCNIGPFAFLRPGTVLGDRVKVGDFVEVKNSRVGEGTKVPHLSYIGDAVLGSGVNVGAGTITCNYDGVAKHTTVIDNGAFVGANSNLVAPVRVGQNAYIATGSTVTTNVPPEALAIARARGVTIVDPLSTWIDVEVTAEPDVVIHPGTQLHGRTHLAEGAEVGPHSTLTDTLVGADAVVANTVCVEAEIGPEATVGPYAYLRPGTRLARKAKAGAYVEMKNAVVGEGSKVPHLTYVGDAEIGEGSNIGASCVFVNYDGVQKHRSVVGDHVKVGSDSMIVAPVTIGDGAYTAAGSVIVKDVPPGAMAVARGRKIER",
    "response#3": "VVVLAAGKGTRMRSSLPKVLHAIADKPMVSHVIDAARQLNAENIYIVYGFGGEVLKATITDNSKSDDLTFIEQKEQLGTGHAVDMASPFIKDDENVLVLYGDVPLTKVSTLQNLLAAKPEGGGYGRIVRDSLGRVLRIVEQKDALETERAVEEVNTGILLVPAAQLKKWLSRLSNNNAQGEYYLTDIIAMAVADGVEVETAQPALAWETEGVNNKVQLAELERIYQHQQAQQLLEQGVTLADPARFDLRGEVSCGRDVYIDVDVVLSGHVVMGEGCQIGSHCVLTNVTLGQNCLIKSHSVLEDCEMADACEIGPFARIRPGTVLASNCKIGNFVETKNAVFDEGSKANHLSYLGDVKVGKAVNIGAGTITCNYDGANKHQTVIEDGVFIGSDTQLVAPVTVGKNATIGAGSTIRKSVPAGGLTLTKSEQRNIANWKRPVKTKK"
  },
  {
    "reference": "MPTAKRTSSRASLALPFQLRLVHKKSWGHRLRDFISGFLKSCRPIAKYVFPNFIVVHYIYLITLSIIGSILLYPCKNTAFIDVLFLAAGASTQGGLATKSTNDFNLYQQIVVYVITLLSTPILIHGFLAFVRLYWFERYFDNIRDISKQNFKLRRTMTLQQRELSGSSGNAARSRSFKDNLFRGKFVSREDPRQSASDVPMDSPDTSALSSISPLNVSSSKEESSDTQSSPPNFSSKRQPSDVDPRDIYKSIMMLQKQQEKSNANSTDSFSSETNGPAFIVQERHERRAPHCSLKRHSVLPSSQELNKLAQTKSFQKLLGLRRDEGDHDYFDGAPHKYMVTKKKKISRTQSCNIPTYTASPSPKTSGQVVENHRNLAKSAPSSFVDEEMSFSPQESLNLQFQAHPPKPKRREGDIGHPFTRTMSTNYLSWQPTFGRNSVFIGLTKQQKEELGGVEYRALRLLCCILMVYYIGFNILAFVTIVPWACTRHHYSEIIRRNGVSPTWWGFFTAMSAFSNLGLSLTADSMVSFDTAPYPLIFMMFFIIIGNTGFPIMLRFIIWIMFKTSRDLSQFKESLGFLLDHPRRCFTLLFPSGPTWWLFTTLVVLNATDWILFIILDFNSAVVRQVAKGYRALMGLFQSVCTRTAGFNVVDLSKLHPSIQVSYMLMMYVSVLPLAISIRRTNVYEEQSLGLYDSGQDDENITHEDDIKETDHDGESEERDTVSTKSKPKKQSPKSFVGAHLRRQLSFDLWYLFLGLFIICICEGRKIE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein can be expressed in cell periphery and perform potassium ion transmembrane transporter activity without triggering undesirable cellular responses.2. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.3. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "SFQSLGVVYGDLGTSPLYVFYNTFPHGIKDPEDIIGALSLIIYSLTLIPLLKYVFVVCKANDNGQGGTFALYSLLCRHAKVKTIQNQHRTDEELTTYSRTTFHEHSFAAKTKRWLEKRTSRKTALLILVLVGTCMVIGDGILTPAISVLSAAGGLRVNLPHISNGVVVFVAVVILVSLFSVQHYGTDRVGWLFAPIVFLWFLSIASIGMYNIWKHDTSVLKAFSPVYIYRYFKRGGRDRWTSLGGIMLSITGIEALFADLSHFPVSAVQIAFTVIVFPCLLLAYSGQAAYIRRYPDHVADAFYRSIPGSVYWPMFIIATAAAIVASQATISATFSLVKQALAHGCFPRVKVVHTSRKFLGQIYVPDINWILMILCIAVTAGFKNQSQIGNAYGTAVVIVMLVTTLLMTLIMILVWRCHWVLVLIFTVLSLVVECTYFSAMLFKIDQGGWVPLVIAAAFLLIMWVWHYGTLKRYEFEMHCRVSMALFRGCSDDFLSQIVLKLHEEFFLPGEVILEQGTVVDQIYIVAHGCLEEVANGEDGSEEIISELRPYGIVGDVAVICNIPQPYTVRVCELCSLLRIDKQSLTSILQIYFKDNSQILSNLL",
    "response#2": "GIGVINTELVSGIPAIFSHFITNLPAFHQVVVFLCVKSVPVPHVKPEERFLVGRVGPKEYRLYRCIARYGYRDVHKDDVEFENDLICSIAEFIRSDKPLNYSPDPENESGINERLTVVAASSSNLEGVQIYEDDGSDKQEPSSSSEVIMVAPSPRFKKRVRFVLPESARIDRSAEEELTELTEAREAGMAFIMGHSYVRAKSGSSVMKKIAINFGYDFLRRNSRGPR",
    "response#3": "GIGVINTELVSGIPAIFSHFITNLPAFHQVVVFLCVKSVPVPHVKPEERFLVGRVGPKEYRLYRCIARYGYRDVHKDDVEFENDLICSIAEFIRSDKPLNYSPDPENESGINERLTVVAASSSNLEGVQIYEDDGSDKQEPSSSSEVIMVAPSPRFKKRVRFVLPESARIDRSAEEELTELTEAREAGMAFIMGHSYVRAKSGSSVMKKIAINFGYDFLRRNSRGPC"
  },
  {
    "reference": "MKVVTVEEMLQLEEVCSKIDLPSEKLMENAGQAVAEQVSFTLDEIEKQHILLLIGPGNNGGDGLVAARHLKDWGADISVYLLSERPDDDPNLKLVRERDITCIEAGQDEKLTKLRELLSSSKAVIDCIFGTGRSRPLEGVFKQALEAVSNAQNNHPLLHTFALDIPSGLDANTGKADPACLHVDDTITLGLPKLGLFNGAGAERAGQITVADIGIPSYLSEYIKTELITWDWVASVLPERPFTANKGTFGRILVVAGSGRYIGAAYLACMGAMRVGAGLVTLATPASLKPILAGKLTETTYLPLPETSDGNISSGAAKLVQQELGNYDVLLMGCGLGQDRQVADFIKNTLLGSKKAQLPLVLDADALNNLATTPKWWQQLHDDAILTPHPGEMSRLAGVSIDEVQSDRVGTAMRMAKEWGKIVVLKGAYSVIANPEGACRISPMANPGLASAGTGDVLAGVIAGLRAQGMTPAEAAACGVYLHAEAGEVMRAEIGDAGMIASDLLPVLPMVIQELKEELESSSTEEEENVASG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..2. The protein must have high specificity towards ADP-dependent NAD(P)H-hydrate dehydratase activity.3. The designed protein should be able to compete with endogenous proteins or inhibitors for AMP binding. The designed protein sequence is ",
    "response#1": "MIGENDLQTLPRPHPPRSAMKYERGRVLVVAGSERYRGAALLALQGVLASGVGSVQAALPQSVAAEIWQLIPEVVLAAALPATDQSGLDWGGWLAGHDLQRLDALLFGPGLGENGGDWAQQAEPLRTFPGLLVLDADGLNQMAASPCGWQWIRGRLGPTLITPHAGEFARVFPKISLDQPLEAARAAAACSGATVLLKGAHPLLASPQGSVHQLISGNPRAARTGLGDVLAGFTAGWGAQVISDQTTQQSLDEMLAAAALLHGEAARSCDQASTARAVASQLN",
    "response#2": "GSHKYARGHVGVFSGGPSASGAARLAAISAARAGAGAVTVLSPKQAVQVNAMHLTAVMLKWIDTPEEAVNFAKDRKMSSAVIGPGFGVGERCVEFLLALAGLGAESPFGLVLDADAITSFADAPDRLFEALAAIGAQTGRERAVLTPHEGEFRRLFPDLADAQGVSKPERARRAAERANAVVILKGPDTVIAAPDGRAAINVNATPFLATAGSGDVLAGIVAGLLAQGMPTFEAAAAGVWLHAEAGARFGPGLIAEDLPALLPAAL",
    "response#3": "MIGENDLQTLPRPHPPRSAMKYERGRVLVVAGSERYRGAALLALQGVLASGVGSVQAALPQSVAAEIWQLIPEVVLAAALPATDQSGLDWGGWLAGHDLQRLDALLFGPGLGENGGDWAQQAEPLRTFPGLLVLDADGLNQMAASPCGWQWIRGRLGPTLITPHAGEFARVFPKISLDQPLEAARAAAACSGATVLLKGAHPLLASPQGSVHQLISGNPRAARTGLGDVLAGFTAGWGAQVISDQTTQQSLDEMLAAAALLHGEAARSCDQASTARAVASQLR"
  },
  {
    "reference": "MKLRELLSDLPGSPSPIAAAHPAWETEVKGLSTNSHACQPGDLFIGMPGTRVDGGEFWPSAIAAGAIAAVVSPAALAARPLAMGSAEPLVIPCDDMARVCAAIAATFYGHPAQQLQTVGVTGTNGKTTTTHLIEYFLTQAQQPTALLGTLYSRWPGHQQTAIHTTPFPIDLQRELAAATAAGSGFAVMEVSSHALAQKRVWGCTFEAAVFTNLTQDHLDYHRDLDDYFEAKALLFSPDYLRGRAIINQTDLYGRRLIQRLPAERVWTYSVDDATADLFTQSLSYAPAGVSGTLCTPAGRQPFRSPLVGQFNLENLLAAVGAALHLGLGLEQIVSALPEFGGVPGRMERVQIPDQDISVIVDYAHTPDSLQNALTAIRPFVQGQLICVFGCGGDRDRTKRPQMGRIAHERSDRAIVTSDNPRTEDPERILQDIVAGVDTALDPNQVLCDRAEAIRSAILQAQPGDGVLIAGKGHEDYQILGTEKIHFDDREQARAALQMRSARS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must be able to regulate cell wall organization.2. The designed protein should have a binding site that is specific to the target ATP ligand. The designed protein sequence is ",
    "response#1": "TGTNGKTSVVDLTAQMLRQTRGAAGSIGTLGARLDQQPQSATNTTPDCLALHSQLSQWRAENVSYVTLEASSHALDQGRLDGLSIDVGVFTNLSRDHLDYHGTMKDYCDAKLRLFRDFNPTTRIYNADDQAVAAHPDIWCEGGLGISAHSGGAEVNFEVLQTAPLTLKLSTPWGGGEIQTALAGRFNAFNIVAAISW",
    "response#2": "AIGVLLTEDLTYAFEDMASVDFLAGVAQAAGDTQLTLIPASPASSVDHVSAQQLVNNAAVDGVVIYSVAKGDPHIDAIRARGLPAVIADQPAREEGMPFIAPNNRKAIAPAAQALIDAGHRKIGILSIRLDRANNDGEVTRERLENAQYQVQRDRVRGAMEVFIEAGIDPGTVPIMECWINNRQHNFEVAKELLETHPDLTAVLCTVDALAFGVLEYLKSVGKSAPADLSLTGFDGTHMALARDLTTVIQPNKLKGFKAGETLLKMIDKEYVEPEV",
    "response#3": "AIGVLLTEDLTYAFEDMASVDFLAGVAQAAGDTQLTLIPASPASSVDHVSAQQLVNNAAVDGVVIYSVAKGDPHIDAIRARGLPAVIADQPAREEGMPFIAPNNRKAIAPAAQALIDAGHRKIGILSIRLDRANNDGEVTRERLENAQYQVQRDRVRGAMEVFIEAGIDPGTVPIMECWINNRQHNFEVAKELLETHPDLTAVLCTVDALAFGVLEYLKSVGKSAPADLSLTGFDGTHMALARDLTTVIQPNKLKGFKAGETLLKMIDKEYVEPEA"
  },
  {
    "reference": "MIKVRVPATSANMGPGFDSMGMAVKVYNEFAFREINNGLNFNGVPEEFCNEDNIIYKAMKYCFDKADYKIKGLNISVLNQDIPISRGLGSSSSCIVGGLVGANEILGGKFSKDELLEMAVKIEGHPDNVAPALFGGMVVAIIENNKTVYNKIEIKDKVKFITIVPDFRLSTEKARTVLPKQISRADGIYNIGRAALMISCFVTDRYDLIRSACNDALHQNYRKELIPHFDDVYNKCYELGALGCYLSGAGPTIMAIIDNGANGFSNNIKQYLKDKDIKWGVLELQADNKGAFVIKGDS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must possess ATP binding to support phosphorylation under certain conditions.2. The ATP binding site should be stable and able to withstand changes in environmental conditions.3. The protein should have a fast turnover rate for ATP + L-homoserine = ADP + H(+) + O-phospho-L-homoserine.4. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate..5. We require a protein that can specifically bind to the metabolites produced in the Amino-acid biosynthesis, L-threonine from L-aspartate: step 4/5 pathway. The designed protein sequence is ",
    "response#1": "MIYSLHDNNVELFGKYMMQDAVVEPCRATLIDNYSEVKEKVKDLVYGITISGSGPAIISLPKKECVLEA",
    "response#2": "MIYSLHDNNVELFGKYMMQDAVVEPCRATLIDNYSEVKEKVKDLVYGITISGSGPAIISLPKKECVLEL",
    "response#3": "MIYSLHDNNVELFGKYMMQDAVVEPCRATLIDNYSEVKEKVKDLVYGITISGSGPAIISLPKKECVLED"
  },
  {
    "reference": "MADAAARQLQYEYKANSNLVLQADVRLIERPRRDEATGEVCSLVGKLDGTRMGDRYQRTKPEKTEERKVKRQKRDEAQYDFERMKGATLLSEGIDEMVGIVYRPKTQETRQTYEVLLSFIQEALGDQPRDILCGAADEILAVLKNDRLKDRERKKDIDSLLGAVTDERFALLVNLGKKITDFGSDAVNALTAAPNNEEQIDETYGINVQFEESEEESDNDMYGEIRDDDAQDEGEEARIDHTLHAENLANEEAANNVKKERSLHPLDIDAYWLQRCLSKFYKDAMVSQSKAADVLKILKDAADDRDCENQLVLLLGYDCFDFIKQLKLNRQMVLYCTMLASAQTDSERQRIREKMRGNSALAKILRQLDTGKSEDQEEGEARGSKRGKGDAEDGGAAAAGQVAGVRQLLELEEMAFTQGSHFMANKRCQLPDGSYRKQRKGYEEVHVPALKPVPFDANEELQPVDKLPKYVQPVFEGFKTLNRIQSRLYKAALDSDENMLLCAPTGAGKTNVALLTMMREIGKHINEDGTINAQDFKIIYVAPMKSLVQEMVGNFGRRLACYNLTVSELTGDHQLTREQIAATQVIVCTPEKWDIITRKGGERTFVSLVRLVIIDEIHLLHDERGPVLEALVARTIRNIETTQEEVRLVGLSATLPNYQDVATFLRVKPDKGLFYFDNSYRPVSLEQQYIGVTEKKALKRFQVMNEIVYEKTMEHAGRNQVLVFVHSRKETGKTARAVRDMCLEQDTLGSFLKEGSASMEVLRTEAEQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein have Helicase C-terminal domains with specific sequence motifs that are crucial for its activity.2. The designed protein should contain at least one DEIH box motif to achieve its intended function.3. For general function, the protein need meet that Plays a role in pre-mRNA splicing .4. A protein that contributes to mitotic cell cycle, mRNA splicing, via spliceosome by functioning in small nuclear ribonucleoprotein complex, spliceosomal complex with ATP hydrolysis activity, nucleic acid binding, RNA helicase activity, ATP binding. The designed protein sequence is ",
    "response#1": "GFEKPSAIQQRAVLPIISGRDVIAQAQSGTGKTSMISLSVCQIVDTAVREVQALILSPTRELAAQTERVMLAIGDYINIQVHACIGGKSIGEDIRKLEHGVHVVSGTPGRVCDMIKRRTLRTRAIKLLILDEADEMLGRGFKDQIYDVYRYLPPELQVCLISATLPHEILEMTSKFMTDPVRILVKRDELTLEGIKQFGATSIKQRSLLNMEAGCDTITLKSSVPIHSLSYAWQSIKEQLGDDVDSKIHRMCLLKDSMGVCFDVRSENLESMQERWTDTKQWQFTVATELPAIAVQRAAIPIALQCRDLIGVAVTGSGKTASFVLPLLVYISQLPPLGPSNRADGPYAIVLAPTRELAQQIEVETRKFAAPLGFNTAVIVGGHSIEEQAFQMRDGAEIVIATPGRLVDCIERRMLVLSQCTYVIMDEADRMIDMGFEEPVNKILDALPVTNEKPDSDAAEDPNAMKRGMYRQTMMYTATMPTAVEQ",
    "response#2": "TPIQAQAIPAIMSGRDVIGISKTGSGKTVSFILPLLRQIKAQRPLGGDETGPLGLILSPTRELALQIHEEVTKFTSGDPSIRSLCCTGGSELKRQINDIKRGVEIVIATPGRFIDLLSLNSGNLINPKRIVFVVMDEADRLFDLGFEPQVNQIMKCIRPDKQCVLFSATFPNKLIGATSIKQRSLLNMEAGCDTITLKSSVPIHSLSYAWQSIKEQLGDDVDSKIHRMCLLKDSMGVCFDVRSENLESMQERWTDTKQWQFTVATELPAIE",
    "response#3": "QLLKAVEEHQVLVIVGDTGSGKTTQIPQYLHEAGYTKRGKVGCTQPRRVAAMSVAARVAQEMGVKLGHEVGYSIRFEDCTSDKTVLKYMTDGMLLRELLGEPDLASYSVVIVDEAHERTLSTDILFGLVKDIARFRPDLKLLISSATMDAERVEQRVEMISDDSKKWRRVEEILESNRFSPPIIIFVNLKRNIEAIAKQLNAIGWHAVTLHGSKSQEQRERAIEQLRNKTADILVATDIAGRGIDIPNVSLVLNYNMAKSIEDYTHRIGRTGRAGKSGTAITFLGPEDTDVYYDLRVLLSRSAKAHIPDELRNHQLLKAVEEHQVLVIVGDTGSGKTTQIPQYLHEAGYTKRGKVGCTQPRRVAAMSVAARVAQEMGVKLGHEVGYSIRFEDCTSDKTVLKYMTDGMLLRELLGEPDLASYSVVIVDEAHERTLSTDILFGLVKDIARFRPDLKLLISSATMDAEMSFIKLTEIQADSIPVSLQGHDVLAAAKTGSGKTLAFLVPVIEKLYREKWTEFDGLGALIISPTRELAMQIYEVLTKIGSHTSFSAGLVIGGKDVKFELERISRINILIGTPGRILQHLDQAVGLNTSNLQMLVLDEADRCLDMGFKKTLDAIVSTLSPSRQTLLFSATQSQSVADLARLSLTDYKTVGTHDVMDGSVNKEASTPETLQQS"
  },
  {
    "reference": "MKTNGKPRAEIDASLFVSVEEALRRFRAGEILIVIDDEERENEGDFVMAAEHATPEAINFMSKYGRGLVCVAITGERARELQLQAMVTTNTALHGTAFTVSVDAIEGTTTGISAFDRAVTVRKLVDPNTRPEELGRPGHIFPLVADPGGVVVRPGHTEAAFDLARLAGCRPAGVLCEILDEDGSMARLPRLQEIARQHGLKIISVRSLMEYRFRTETFIERLSEVKLPTRYGEFRLALYKNRLNPEDHHMALVKGHWEADEPVMIRIHSECFTGDTLGSLRCDCGDQLHLAMKRIQRAGKGVLLYLRQEGRGIGLPNKILAYKLQDEGKDTVEANLALGFKADLREYWLAAQMLKDLGVQKVRLMTNNPRKVNDLKKYGIQVVERIPVIVKPNPANEKYLRTKQEKLGHMIPLDHDNN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must possess GTP binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, zinc ion binding, GTP cyclohydrolase II activity, magnesium ion binding to support riboflavin biosynthetic process under certain conditions.2. The designed protein should contain one or more GTP cyclohydrolase II domains that are essential for its function.3. The D-ribulose 5-phosphate, Mg(2+), GTP binding site should be located in a region of the protein that is accessible to the ligand.4. A protein that can modulate the overall flux of metabolites through the riboflavin biosynthesis, Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed.5. The Proton acceptor; for GTP cyclohydrolase activity, Nucleophile; for GTP cyclohydrolase activity of the protein must be engineered to enable effective catalysis of GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.6. The presence of Zn(2+) is necessary for the protein to function in the desired environment.7. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.. The designed protein sequence is ",
    "response#1": "GVDMPTQWGDFSLIAYTQVATGDTHLALVKGEWAPGEPVLTRVHSSCVTGDIFGSCRCDCGGQLHRAMTMVEEAGKGVVLYMFQEGRGIGLLNKLKAYKLQEMGRDTVEANLELGFKMDERDYGVGAQILRDLGVSKLRLMSNNPKKRNALAGYGLEITETVEVFDHPIFADAASARMPTKYGDFQIYGYVNDITGEHHVALVKGDLGDGENVLCRVHSECLTGDTFGSRRCDCGEQLQRAMQQVEKEGRGIILYMRQEGRGIGLINKLKAYELQEQGLDTVEANIRLGFDADLREYWVGAQILADLGARSLRLLTNNPDKIYGLEGFGLTITERVPSLPYDDAWPIAEREARVAETSIPNRYGTWRAYGYTSSLDGGEHLALVLGEGLEENVLVRAHSECLTGDVLGSLRCDCGVQLEHAMEAIAAEGRGVIVYLRGHEGRGIGLVAKLRAYSLQDNGSDTVDANLELGLPVDAREFSNAGQILGDLGVKSVRLLTNNPAKLRGMDGYGIKVLGRDATLQLPTGATVRLDKDGRPVLDGDGAPVTAASDRHEDN",
    "response#2": "MVEREVEINLPTSYGDFRTIAYTNQIDGREHIALVKGTISPEEPTLVRVHSECLTGDVFGSHRCDCGPQLESALQQIEEAGKGVLLYMRQEGRGIGLLNKLKAYKLQEEGYDTVEANEKLGYAADLRDYGIGAQILEDLGVRKMKLLTNNPRKIKGLHGYDLELVDRVPLAEPVGGAETSLHTPYGDFRAIAFVNDIDDYEHMALVKGDIGPDMDILVRVHSGCLTGDVFGSYRCDCGEQLKRAMSMVQEEGRGVILYLQQEGRGIGLANKLKAYALQDRGYDTVEANEELGFAADLRDYGVGAQILVSLGVRKMKLITNNPKKIVGLEGYGLKVAGRVKAPTPVKGKAPKIAEVELPTTRGFFRAFAYRDINDENLEHVHIALVKGDLRGRENILVRVHSECLTGDVFGSLRCDCGSQLAEAMNMIEKEGLGALLYMRQEGRGIGIAEKLKAYKLQEEGMDTVEANEALGFPADLRDYGIGAQILQDLGVKSIRLITNNPRKVVGLQGYGLEIVERVPLEEPVGKILKVKLPTEHGEFEFIPFRQTSNGLEHAALVKGKWTRDEPVLVRLHSSCFTGDIFGSLRCDCGSQLHKAMEMVEAAGKGVVIYLSQEGRGIGLFNKIKAYKLQDQGMDTVQANIKLGFGEDERDYGVGASIMRELGLGKIRLISNNPVKRAGLEGYGIKIVEAVPLEEPVGKVLKVERVSSVHLPTIYGDFMAHAYRFILDESPERLHIALVKGDLTAETPVLVRVHSECLTGEVFGSLKCDCGQQLHKAMELIEKEGMGVLLYMRQEGRGIGLLPKLKAYELQEKGLDTVEANLALGYPPDLRDYGVGAQILVHLGLRKIRLMTNNPKKIVGLRGYGLEVVERV",
    "response#3": "MKLGQLLAQAGLQPEGDAVEVVLDQVCSDSRKARPGTLFVAVRGAEFDGHDFVAEALTKGAVAVVGERGCDRGGAWGDVPLIQVADTRAALALAAAEVYGNPSRQLTLVGA"
  },
  {
    "reference": "MKVSTVAQMRAMDRAAVEHYGIPETLLMENAGLAAFEALSRNVCISGRSFVVLAGMGNNGGDGFVVARKIHSAGGFVKVIILGEMSRYRGAARVNLDIIRKMPIRLSEASNARSVQRDLSGCDAIVDAVFGTGLAREVGGMHRQVIEAVNASGKYVLSLDIPSGVAGDTGRIMGCAVRADLTVTFGLPKAGTILLPGYGLCGKLHVSHISFPPDLYDSDDLPMSLNVPPPLPERNPDGHKGSFGEVLFIAGARGYYGAPGFAAMSFLKAGGGYARLAAPESIIPVIAASGSEVVFHPMSETDTGSISPRNKTRLLELADRVDMVVIGPGISLNDETVGLVRELSRRIARPLLIDGDGITAVSGDLACIRKRKAATVITPHLGEMARVTGRPIKTIEEDRISHLRETAEDLHAVVVLKGAHSLTGYPDGRISMTLSGNPGMATAGSGDVLTGTIAAMHGLGLNLHDAVRTGVFVHGAAGDLAASTHGEDGITAGDILEGLPRTMLLYRQGSLAQRYVLPEV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein with ADP-dependent NAD(P)H-hydrate dehydratase activity functions is required to enable nicotinamide nucleotide metabolic process.2. The designed protein should have a binding site that is specific to the target K(+) ligand.3. The protein should be designed to catalyze the following reaction (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.4. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.5. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "MRACDLNTEENGIPTLTLMENAGFQITQHIINNYPDKKRLSIYSGVGGNGGDGFVVARLLLNHGYKVHLKLLARPENIKNKDSITNWKALNNCLKADTNLKITIIRDSKDIVPDNADIIVDAILGTGVNGKIREPISSAIDTINNSPAVVLSVDVPSGLDPLTGDVFDKCVIAHNTLTLHKPKTGLTADVKQYTGELSVLDIGIPRVSESVVGGEMPGAAALCASAAMRAGAGYVRLIADAFVPDVPQAVVQSPLCSGAGRSPAAPKGETGLRPAPEHNDDVAALLADPRVNALLAGPGLGRSGRSRSLLHQAIEARHPLILDADALNLVGEDWGLFSDSSELPILTPHGGEFARLFGTLAGSKVEQARAAAVRANAVILLKGADTVVAGPDGRAAISAAAPAWLATAGTGDVLAGIVAAMRARGMPPFEAACAAVWLHGRAAELR",
    "response#2": "MRACDLNTEENGIPTLTLMENAGFQITQHIINNYPDKKRLSIYSGVGGNGGDGFVVARLLLNHGYKVHLKLLARPENIKNKDSITNWKALNNCLKADTNLKITIIRDSKDIVPDNADIIVDAILGTGVNGKIREPISSAIDTINNSPAVVLSVDVPSGLDPLTGDVFDKCVIAHNTLTLHKPKTGLTADVKQYTGELSVLDIGIPRVSESVVGGEMPGAAALCASAAMRAGAGYVRLIADAFVPDVPQAVVQSPLCSGAGRSPAAPKGETGLRPAPEHNDDVAALLADPRVNALLAGPGLGRSGRSRSLLHQAIEARHPLILDADALNLVGEDWGLFSDSSELPILTPHGGEFARLFGTLAGSKVEQARAAAVRANAVILLKGADTVVAGPDGRAAISAAAPAWLATAGTGDVLAGIVAAMRARGMPPFEAACAAVWLHGRAAELR",
    "response#3": "LLVVAGSDRYRGAALLSLLGASASGCGSLRAALPAAVAAGLWAVQPQVVMEAALEERPTGGLALAPLAVPGVLDRLDAVLLGPGIGLGSGIGGGTACADEPAWQALQDFPGLLVLDADGLNRLAGGAAGTAGTTWLQQRRGPTWLTPHAGEFARLFPDLAGRPPLEAAAAAAGRCQVSVLLKGARSVVASADGRRWQLLAAAAAAARAGLGDVLAGYAAGRGAAAGCGEGGALLAAAALEHAQAGLACCKELGEGAATPQAIAAADLKTAPRPVLPPEAAKYGRGRLLVVAGSDRYRGAALLSLLGASASGCGSLRAALPAAVAAGLWAVQPQVVMEAALEERPTGGLALAPLAVPGVLDRLDAVLLGPGIGLGSGIGGGTACADEPAWQALQDFPGLLVLDADGLNRLAGGAAGTAGTTWLQQRRGPTWLTPHAGEFARLFPDLAGRPPLEAAAAAAGRCQVSVLLKGARSVVASADGRRWQLLAAAAAAARAGLGDVLAGYAAGRGAAAGCGEGGALLAAAALEHAQAGLACCKELGEGAATPQAIAQQLAGRAGRL"
  },
  {
    "reference": "MNLRLCVQALLLLWLSLTAVCGGSLMPLPDGNGLEDGNVRHLVQPRGSRNGPGPWQGGRRKFRRQRPRLSHKGPMPF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Inhibits HIV-1 entry in cells coexpressing CD4 and APLNR; Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives..2. A protein with apelin receptor binding functions is required to enable negative regulation of blood pressure, immune response, positive regulation of G protein-coupled receptor internalization, negative regulation of gene expression. The designed protein sequence is ",
    "response#1": "GNGLVIVIATFKMKKTVNTVWFLNLAVADFLFNVFLPIHIAYAAMDYHWVFGTAMCKISNFLLIHNMYTSVFLLTVISFDRCISVLLPVWSQNHRSIRLAYMACVVIWVLAFFLSSPSLVFRDTAHLHGKISCFNNFSLSATSSSSWPTHPQMDTVGFGRQMVVTITRFLCGFLVPVLIISACYFTIVYKLRRNRLAKTKKPFKIIVTIIITFFLCWCPYHTLYLLELHHRAMPGSVFSLGVPLATAIAIANSCMNPILYALVSKHFRRGFRQILCRGPC",
    "response#2": "GNGLVIVIATFKMKKTVNTVWFLNLAVADFLFNVFLPIHIAYAAMDYHWVFGTAMCKISNFLLIHNMYTSVFLLTVISFDRCISVLLPVWSQNHRSIRLAYMACVVIWVLAFFLSSPSLVFRDTAHLHGKISCFNNFSLSATSSSSWPTHPQMDTVGFGRQMVVTITRFLCGFLVPVLIISACYFTIVYKLRRNRLAKTKKPFKIIVTIIITFFLCWCPYHTLYLLELHHRAMPGSVFSLGVPLATAIAIANSCMNPILYSFRNKELRAAVRRTVG",
    "response#3": "GNGLVIVIATFKMKKTVNTVWFLNLAVADFLFNVFLPIHIAYAAMDYHWVFGTAMCKISNFLLIHNMYTSVFLLTVISFDRCISVLLPVWSQNHRSIRLAYMACVVIWVLAFFLSSPSLVFRDTAHLHGKISCFNNFSLSATSSSSWPTHPQMDTVGFGRQMVVTITRFLCGFLVPVLIISACYFTIVYKLRRNRLAKTKKPFKIIVTIIITFFLCWCPYHTLYLLELHHRAMPGSVFSLGVPLATAIAIANSCMNPILYCFMNRRFMGTRHKTLIRIMVSRSEIDMNEIKVFYQKKYGIPLCQAILDETKGDYEKILVAYT"
  },
  {
    "reference": "MENIISIVKKDLKEIAQKFNISKDPIVEINKNNIDSHFSTTLALMSAKELKQNPIQLAENIKNELLQKDYYDQIEIAGPGFINIKLKTELLSTTIKNITTLKEAYGKNKIKNKIINIEYVSANPTGFLHVGHARNAVTGSVLEEVLKFDGYEVQTEFYTNDAGNQINILAVTVFVHYLWALGIETEKPANTYGGTFYDDLANIMIQKYGDKFKNLTFTETAISDEETHQIFRKEAVQHFLTEIKQQLKDFGVVIDHYSSEQEMYDTNQIEKLLKEYKEKNATYEADGALWLKTTEFGDDKDRVLVKKDGSLTYIVPDLATHNIRIQRTHADVLINIWGGDHHGYIPRMRAGLQLLGHNPDILEIEMVQMVRLIKDGKEYKMSKRKGTAVWLVDIMEMVGKDALRYMLASKSSSSHMDLDLDLVQQKNATNPVYYAQYATARCHSILNQADQKNIKANLNVSNLLSNKKEVELLLTLDNFNQVIQMSAKNRAPQLICEYIQTVCKQFHSYYADTKILDENDVPTSEARLGLVLSVLQVLTNAFNIIGVSALETM",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be able to regulate arginyl-tRNA aminoacylation.2. We are looking for a protein with broad substrate specificity for ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-   tRNA(Arg). The designed protein sequence is ",
    "response#1": "VQYAHARICSVLAAWGGDAATLKDVDLSALEGPQAQALMLQLAKYPAMLTSAAEGNAPHDVTFYLRELASLYHSYYDAERILVDDEKVKLARLALISATRQVLHNGLAVLGVSAPQRL",
    "response#2": "VQYAHARICSILRMAKERGIELPSFENVEPQLLRIPEEIALIKTLTRFPEVVEGSARTLEPHRITFYLNDLAGLFHSYYNKYKVISDDEAMTRTRLFLVKCIQTVLKNALTLLGVSAPEKL",
    "response#3": "VQYAHARCHSVMRHAAGMWPDAVGPVPGVEVLAHLTDPAELGLIRLLAGWPRLVESAAEAHEPHRVAFYLYDLAAAFHGLWNKGKDDTSLRFLIEDDRELSLARLGLLRAVVGVIASGLGIFGVAPVEE"
  },
  {
    "reference": "MDLVDPDLDPWNHPGSQPKTPCTKCYCKVCSYHCQVCFLNKGLGISYGRKKRRQRRRTPPSNQDHQNPIPEQPTPQARRNSAGSEESKKKVESKAESDRFD",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive; Besides its effect on RNA Pol II processivity, Tat induces chromatin remodeling of proviral genes by recruiting the histone acetyltransferases (HATs) CREBBP, EP300 and PCAF to the chromatin; In the absence of Tat, the RNA Pol II generates short or non-processive transcripts that terminate at approximately 60 bp from the initiation site; Other factors such as HTATSF1/Tat-SF1, SUPT5H/SPT5, and HTATIP2 are also important for Tat's function; To ensure maximal activation of the LTR, Tat mediates nuclear translocation of NF-kappa-B by interacting with host RELA; Through its interaction with host TBP, Tat may also modulate transcription initiation; Tat can reactivate a latently infected cell by penetrating in it and transactivating its LTR promoter.2. The protein should exhibit Basic and acidic residues compositional bias.3. The designed protein should contain at least one Nuclear localization signal, RNA-binding (TAR), and protein transduction motif to achieve its intended function.4. The designed protein should have a binding site that is specific to the target Zn(2+) ligand. The designed protein sequence is ",
    "response#1": "MEPVDPRLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFTKKALGISYGRKKRRQRRRAHQDSQTHQVSLSKQPASQPRGDPTGPKESKKEVERETETDQDPDH",
    "response#2": "MENRWQVMIVWQVDRMRIRTWNSLVKHHMYVSKKARGWFYRHHYESRHPKISSEVHIPLGDAKLVVTTYWGLHTGERDWHLGQGVSIEWRKRRYSTQVDPDLADQLIHLYYFDCFSESAIRNAILGHIVSPRCEYQAGHNKVGSLQYLALTALIKPKKIKPPLPSVRKLTEDRWNKPQKTKGHRGSHTMNGH",
    "response#3": "MDPVDPNLEPWNHPGSQPKTPCNKCYCKSCCWHCQVCFLKKGLGISYGRKKRRRRRGPHGSQTHQVPLPEQPLSRTQGDSTGPKESKKEVESKTETNPFD"
  },
  {
    "reference": "MGTIKIVTDSSITIEPELIKALDITVVPLSVMIDSKLYSDNDLKEEGHFLSLMKASKSLPKTSQPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTIEASRQGAEIAETPVTVLDSGFTDQAMKFQVVEAAKMAKAGASLNEILAAVQAIKSKTELYIGVSTLENLVKGGRIGRVTGVLSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLDSYLAKNSHRPIAEIAISYAGEASLALTLKERIAAYYNHSISVLETGSIIQTHTGEGAFAVMVRYE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: May bind long-chain fatty acids, such as palmitate, and may play a role in lipid transport or fatty acid metabolism..2. The protein must exhibit lipid binding. The designed protein sequence is ",
    "response#1": "MK",
    "response#2": "GWI",
    "response#3": "MQIV"
  },
  {
    "reference": "MADNDNEDIIMDDLVEEYVETEEENFVDSEEESEDKDEIVESPSICEGFVQASSQTLVIIPDNERITSNVLTTFEATRLVAVRAQQLAINGSTMLKKKYSSPIDIAKQELFNRKIPLLVMRCIKVTPEGQKIVEIWNPREMGIPLLD",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Component of the DNA-directed RNA polymerase (RNAP) that catalyzes the transcription in the cytoplasm of viral DNA into RNA using the four ribonucleoside triphosphates as substrates..2. The designed protein should be involved in DNA-templated transcription, viral transcription by localizing to DNA-directed RNA polymerase complex, host cell cytoplasm, virion component and exhibiting DNA-directed 5'-3' RNA polymerase activity, DNA binding. The designed protein sequence is ",
    "response#1": "NKDSSSKVVSSEFDLIPGVSEDTLEVIANIKNIHLKLGEGEQRKIISFSVSGKDTKVLKASHFERDGVEVFNRDLVIATLSQDVNLDLEFQINYGRGYVSSEQNSKYLEEVNVIALDSIFK",
    "response#2": "NKDSSSKVVSSEFDLIPGVSEDTLEVIANIKNIHLKLGEGEQRKIISFSVSGKDTKVLKASHFERDGVEVFNRDLVIATLSQDVNLDLEFQINYGRGYVSSEQNSKYLEEVNVIALDSIFI",
    "response#3": "NKDSSSKVVSSEFDLIPGVSEDTLEVIANIKNIHLKLGEGEQRKIISFSVSGKDTKVLKASHFERDGVEVFNRDLVIATLSQDVNLDLEFQINYGRGYVSSEQNSKYLEEVNVIALDSIFS"
  },
  {
    "reference": "MNDVSKTGLPKAIFLMGPTASGKTALAIRLRQTLPVELISVDSALVYKGMDIGTAKPDAHELSQAPHRLLDIIDPAQAYSAADFRRDALQAMAEIVAAGRIPLLVGGTMLYFKALLEGLSPLPSADPTIRAEIERQAAEQGWDALHRKLQDIDPVAAQRIHPNDPQRLSRALEVFFISGKTLTELTQTSGEALPYQVHQFAIAPASRELLHQRIEQRFHQMLASGFEAEVRALFARGDLHTDMPSVRCVGYRQMWSYLAGEISYDEMVYRGICATRQLAKRQITWLRGWEGVHWLDSEKPEQAYSDVLQVIGAKQQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be able to bind substrate, ATP ligand in a variety of conditions2. The protein must exhibit ATP binding. The designed protein sequence is ",
    "response#1": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#2": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR",
    "response#3": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYI"
  },
  {
    "reference": "MAKTSLKVKATRHPKYSARAYTRCQICGRPQAVLRKYKICRICFRKLAHEGKIPGMKKASW",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that can perform rRNA binding, zinc ion binding in ribonucleoprotein complex to support translation.2. Zn(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "MKNCSFCGKAIEPGTGIMYVRKDGAILYFCSNKCRKNMIELNRVPRYVKWTNEYHELKKMRL",
    "response#2": "MFGFGYNN",
    "response#3": "MPCSFCGKAIEPGTGIMYVRKDGAILYFCSNKCRKNMIELNRVPRYVKWTR"
  },
  {
    "reference": "MLNREQLISIRRDLHQIPELGFKEFKTQAYLINHLDAYSKDRIEMETWRTGLFVKVKGTNPERVFAYRADMDGLSIPEDTGYPFQSVHEGKMHACGHDLHMTIALGVIDHFVHEPIKEDLLFMFQPAEEGPGGAEPMLTSDVLKKWTPDFITALHIAPEYPVGTIATKPGLLFANTSELVIDLEGKGGHAAYPHLANDMVVAASALVGQLQSVISRNVDPLDSAVITIGTITGGTAQNIIAQHAKLDGTIRTLSPESMEKVRKRIEALAKGIEIGYECKATVRYPSSYYEVDNSKELTEEFMSYVAEEGLANVVECREAMTGEDFGYMLKKYPGFMFWLGVDSEYGLHHAKLMPDEKAIETAVNVMTAYFKKQAGE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that is capable of catalyzing reactions within the Amino-acid biosynthesis, L-lysine biosynthesis via DAP pathway pathway with high efficiency and specificity.2. A key design requirement for the protein is the optimization of the active site to achieve high catalytic efficiency for H2O + N-acetyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)-2,6-   diaminoheptanedioate + acetate.3. The protein must be able to N-acetyldiaminopimelate deacetylase activity. The designed protein sequence is ",
    "response#1": "MPIGVPKVPFRSPGEEDASWVDIFRQLYLE",
    "response#2": "MYKVIRLGIGDVTRPLAPAIIKAFHDAVDDLATIDNFAGYGPEQGYDWLINAIIEKSYKPLGVSLKTEEMFISDGSKCDCANILDIFALDNVVAIGDPVYPVYNDTNVMIGRTGDADEKGYYKGIVYMPCTEANGFIPSLPTEKVDIIYLCFPNNPTGTVATKAELKKWVDYANANDAVIFFDAAYEAFITDPAIPHSIYEIEGAKKCAIEFRSFSKTAGFTGVRCGLVVVPEEVMGTTPTGEKYSFNKLWLRRTTTKFNGASYPVQKAAAAVYSDEGWKQNKEIIDYYMENARIIREGLAAAGLTVYGGVNAPYIWLKTPGGMSSWDFFDKLLTECNVVGTPGSGFGPSGEGFFRLSAFGHRENVIEAVERLRV",
    "response#3": "MLAALIVAAGRGSRAGGDQPKQWRPLAGRRVADWTLAAFRAAGTDAIVVVIHPDDAALYAGHPALTVVAGGASRDASVRRALEALGDAAPDDLAFGGHVDVRPCVSTELIAAVTDALATRAAAPGLAVTDALWRGADGQVTGTQDRTGLFRAQTPQGFHLAAIRDAHRTWPGEDAADDVEVARAAGHDVVLTPGDVANLKITTAADFARAEALLRG"
  },
  {
    "reference": "MSILKDKHIVLGVTGSIACYKSIDLASKLTQAGALVDVILTDGAEKFVTPLTFQAVTGRPVYTDMWKTDGADSLPTHIAHVGLGEDADLLAIIPATANTIAKLAYGLADNLLTVTALASRAPMVIAPAMDAGMYQHPTVQDNITRLIERGGLLIPPEEGRFASGLVGVGRLPETPTLIGHIRRIIGRDGELASQKVVISAGGTREAIDPVRFISNRSSGKQGHALAQAAVDAGAREVVLVTTADLPVPVGVHAILVESAEEMLRAILHHSTDADLLIMAAAVADFKPQQRAEQKIKKENSDKSLVLELGRTADILVEVKAQREQTGYPRMVVGFAAESENVLSNARSKLIRKGLDLLVANDITADDAGFSVDTNRVVILDTEGGMDELGLASKTIVSERIIARAAMMLGSE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be optimized for metal ion binding, FMN binding, phosphopantothenoylcysteine decarboxylase activity activity in order to improve pantothenate catabolic process, coenzyme A biosynthetic process.2. A protein that is capable of catalyzing reactions within the Cofactor biosynthesis pathway with high efficiency and specificity.3. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "RVVVGVGGGIAAYKACELVRELGRAGAEVRVAMTESARQFVTPLTFQALSGHPVLTDYFDPAQEGNFGHLDLARWGEAFVVAPATADLLARIRAGLANDAVTTSLLAFKGPVVLAPAMNVAMWDNPLTQENVAALLKHPRFSQVGPGAGLLACGDVGEGRLADVPAIVAAVARLLGGAGPRPVVPLAGKKVVVTAGGTREALDPVRYLSNRSSGKQGYAIAQAAIDAGARVVLISSAQHLPTPVGAQLVPVESAETMCRAALEHVLGAAALVMVAAVVDYRPRQVAQRKIKKTDSALRLELARTDDILLAVKAQRAASSYPNIVVGFAAESDNLLENARGKLRGKGLDLVVANDISAPDAGFAVDSNRVTVLGADGSVNDLPLTTKEDLADGILDAVVRVRG",
    "response#2": "MKILLVVGGGIAAYKACELVRLIRKGGGDVTCVVTEGGQQFVTPMALAALSENPVHTTLWDLKNEAEMGHIQLSREADLVVVCPATADLLAKMAAGIADDLATTLILATDKPVMAVPAMNVRMWEHQATQRNIEWLKQAGVEVLDPDQGAMACGEFGYGRLPEPEAIWAAIERALS",
    "response#3": "KRILLGVCGSIAAYKAVDLASKLTQADAEVDVIMTVAAQRFVSALTFQAATGRSVYSDLWTSGASGGLPSHIAHIGLAEAAGLFIIAPATANTLAKLAQGMADDMLTVSALAAACPLLLAPAMDGNMYEHPATQANLQTLQSRGAQLIPPESGRLASGLSGRGRLPETATLLGHCLDGHKFIVTGGRTEEDLDPARVVTNRSTGLMGSELLQAAYCRGGAVKGIFGEVTCPLPEDVEITRVRTSTEMLDALKKIFHWCDYLIMAAAVGDYKPRRKNAVKVHGSKYNVALEKNVDLLKEVARQKADKQLVGFSLEDRDGLARAKEKMKAKNLDLVVLNTTAG"
  },
  {
    "reference": "MRKLTEKKLLVATHNRGKLDEIRAMMAPHGIEVTSAGEMGLPEPAETESSFIGNARIKARAAMQATGLPVLADDSGITVDGLDGAPGVYTADWAETPQGRDFMQAMTRTWRELDERGVAEPRSAQFRATLILLWPDGHEEIFEGVAPGRLVWPPRGQQGHGYDPIFVPDGHDLTYAEMPPEQKNAISHRARAFRKLEALFA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be optimized for metal ion binding, nucleotide binding, ITP diphosphatase activity, dITP diphosphatase activity, XTP diphosphatase activity activity in order to improve purine nucleoside triphosphate catabolic process.2. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP The designed protein sequence is ",
    "response#1": "SEYIEGTSLNLISNEKYPFEDVAMLLKKIHNVEWKSDNDYNPFKRLEKYEQYCIEKGFVLPKSYLNIRDKFFTYKDYLQSLPKSFAHGDSQPSNFIITDQGLKVVDFEFTGNIDHIYDIACFANMRISDGEQLLKAKK",
    "response#2": "SEYIEGTSLNLISNEKYPFEDVAMLLKKIHNVEWKSDNDYNPFKRLEKYEQYCIEKGFVLPKSYLNIRDKFFTYKDYLQSLPKSFAHGDSQPSNFIITDQGLKVVDFEFTGNIDHIYDIACFANMRISDGEQLLKAVKALG",
    "response#3": "MPSEYIEGTSLNLISNEKYPFEDVAMLLKKIHNVEWKSDNDYNPFKRLEKYEQYCIEKGFVLPKSYLNIRDKFFTYKDYLQSLPKSFAHGDSQPSNFIITDQGLKVVDFEFTGNIDHIYDIACFANMRISDGEQLLKAFR"
  },
  {
    "reference": "MIESNSVVNRIGAQEPNTVKHIFVTGGVASSLGKGLTASSLGHLLTQRGIKVAMQKLDPYLNVDPGTMNPFQHGEVFVTEDGAETDLDIGHYERFLDIDLDGAANVTTGQVYSSVIAKERRGAYLGDTVQVIPHITDEIKSRMRAQAERPAEDRPDVIITEIGGTVGDIESQPFLEAARQVRHDVGRDNVFFIHVSLVPYLGPSGELKTKPTQHSVAALRSIGIQPDSIVLRSDRDLPDSIRAKIASASDVDLEAVVPCVDASSIYEIPKVLHDGGLDVYVIRRLNLPFRDVDWTKWDWVLERVHNPAQEVRIGVVGKYIDLPDAYLSVTEALRHGGFANDARVKIEWIQSDECDSEAGAAAALSGLDAICIPGGFGVRGIEGKVGALKYARENGIPTLGLCLGLQCMVIEYARHAAGIEGASSSEFDPDTKFPVVATMAEQMSIVDGEGDLGGTMRLGTYQAKLTEGSVAAGAYGATDISERHRHRYEVNNAYRDELAEAGLQFSGTSPNGELVEFVELPADVHPYYVSTQAHPELKSRPTKPHPLFAGLVAAALANR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Nucleophile active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The designed protein should have glutaminase activity, metal ion binding to facilitate glutamine metabolic process.3. The protein should be able to bind UTP ligand in a variety of conditions4. The presence of CTP synthase N-terminal domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "LPDAYLSVTEALHHSGVYFGHNVKIDLIAGDELTSENADAALEGYDGILVPGGFGQRGVEGKILAAQYAREHKIPYLGICLGLQVAVSEFARNVAGMPGANSAEFAPDSPYPVIALMPDQEDVTDKGGTMRLGAYPCKVKHDTLAYEAYGADLIYERHRHRYEVNNAFRQQLQDAGLVISGLSPDDRLVEMVELPESVHPWYVASQAHPEFKSRPTKPAPLFREFVRS",
    "response#2": "VTIAVVGKYTGLKDAYKSLIEALMHGGIANRVRVNLEWIEAEVFERKDAAPYLENVNGILVPGGFGERGSEGKMAAAKFARTRNVPYFGICFGMQMAVIEAARNLAGIEDASSSEFGPTSQPVVGLMTEWLRGNMLEKRAAQGDLGGTMRLGAYPATLKPGSHISAVYGSETIEERHRHRYEVNIDYMERLEACGMQFAGMSPDGVLPETVEFPDHPWFIGVQYHPELKSRPFDPHPLFRSFFAAATGLAE",
    "response#3": "LPDAYKSVVEALSAAGFAHRAKINLRWVESDDCESADGTAKALAGVDAVVIPGGFGNRGVPGKIGALTYTRTRGIPTLGLCLGMQCMAIEGLREIAGLVGADSQEFDAAAEHPVISTMADQTDIVAGRGDMGGTMRLGSYEAKLAEGSIAAELFGTTEVTERHRHRYEVNNAYREQLTKSGFVISGLSPDGRLVEFIELDRDVHPYYVGTQAHPELKSRPTRPHPLFDGLIRATL"
  },
  {
    "reference": "MELKELTKLLTVSRTVGDTNVEVKGVEMDTRKIMGGEVFVCVTPRRGLLKDRHDFAQEAVGKGAVALVVERDVDVDVPKIFVKDTRLALAVMATHFNDHPSESIGLIGVTGTNGKTTTSYIIDHILESAGYKNGLMGNNIMKIDGQWKETAINTQEPPDLQRNLRQMRDLKHDSCVMEVTSQGLDMKRITGCDFKTAVFTNLTQDHLDYHGTIEDYKRAKALLFSGMGNSFNATKRKHVVLNIDDEAYEYLEQMVSCEVITYGIENECDVRAKDIEITPNGMEFLLSTFKGDYKVSSPLIGRFNIYNSLAAIATCIAEGVSLEKITECLSTTPNIAGRMEVVSDNQDYTAVVDFAHTPDALENVLSSIRELSKGNILTVFGCGGDRDSTKRPIMGEVASNYSDHVIVTSDNPRTENRDRIIEDIQSGIDQQTSVNIVPDRQEAIALALRLAQAEDVVVIAGKGQESYQLINNEKLPFDDKETARKLMETVSR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein contains novel Mur ligase C-terminal, Mur ligase central domains that confer a unique function or activity.2. The designed protein should have UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, ATP binding, magnesium ion binding, tetrahydrofolylpolyglutamate synthase activity within cytoplasm to regulate cell division, cell wall organization, cell cycle, peptidoglycan biosynthetic process, regulation of cell shape.3. The designed protein should have a high specificity for ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding, with minimal off-target effects.4. The protein must bind to Mg(2+) in order to perform its enzymatic function.5. A protein that can modulate the overall flux of metabolites through the peptidoglycan biosynthesis, Cell wall biogenesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MTLDSREAGPGVLFVAVAGAHVDGRAFISQALASGVDAVLCDADTMPEDVPLQDARVLGVPGLRGRLGARVTGTSGKTSTAWFTQGLFAATGHTAGYLGTLGLHVPGLPEQYTLTTPDPVALHLYLAQAADAGVTHFALEASSHGIDQRRLDGVRLKAAAFTNLQRDHLDYHATMAAYFEAKARLFLDLLPENGTAVVHAGDDWGQVLRDRVQQRGQTCLTYGTKTADLALTGRALAPGGQALTISALGETAEVIFPVLGSFQAENLLAALGVLLARGVSLETALDRLAQLKGVPGRLEQVETGRDFHVVVDYAHKPDAVEAALSTLRPLTAQAGGRLICVIGAGGDRDTGKRPVMGEIAARLADLVIVTDDNPRSEDPAVIRAAVLEGTRDGAGEVREVGDRREAIRVALSSAGPGDIVLVAGKGHEDYQEIDGVRHHFSDVEEARRALQARRR",
    "response#2": "MKLRELLDFIDYKILEGSLDLSVTGITADSRAVRTGYLFAALSGSKADGRAFIAQAVTMGASVILGAEGSAGHEAAGEPRAAALILRDPRAALGIMANRFLGNPSLTLKLVGIGVTGTNGKTTTARMLRHLLDCSGKKTGLIGTIQMRYQDKIYPSANTTPEAVDLQQYFARMAEESCDHVVMEVSSHALALGRVEGTSFDYGIFTNLTQDHLDFHKTMEEYWRAKLKLFTMCRSAVVNADDPYGRRLEQELEGRCFTYGMERGADFRAENIRLDAGGSEFTLVWNGGSEKIRIPLVGRFNVYNALCAIAVCRLMEVPLENALKSLAEIPGVPGRFETVDAGQNFTVIVDYSHTPDSLENALSTINEFAEKRIITVVGCGGDRERGKRPIMAKIAADRSDLAILTSDNPRTEDPGVILREMEAGVAGRTYKKIEDRREAIRFAVRSAREHDIILIAGKGHETYQIIGKEKHHFDDKEIAAEALKERARHG",
    "response#3": "MFALKDILYKVTLKAVYGSTEVAVTGIQTDSRKVRPGDLFLCIPGLVSDGHDYAAKAQEAGAAALVLERDVPEGVPSVAVADTRLAMGLVSANFYGHPAEKLQMIGVTGTNGKTTTARMLRHLLDCSGKKVGLLGTIENRIGDRIIPAKNTTPDSLELQRLFAEMVEAGCDSVVMEVSSQGLMLHRVAGFTFDYGIFTNLTQDHLDFHGTMERYADAKALLFSQLGSNKNGESTKKYAIVNRDDAAAEYFAAAVTGTNGKTTTTSLLEYLWSGSGLPAGVIGTLGARYHTQGKTVSLPSARTTPEASELQKNLALMRDSGVQVVAMEVSSHALSLHRVDGIYFELSLFTNLTQDHLDFHHTMEEYFLAKSALFTSKFSAGALVNADDSFGKRLLAQCEIPAISISTDKGDWQLSLTGFQLTRPGGAIHIIDSPLVGDFNRMNAAMALVVTGISLSTQRIHPGDLYAALPGTRAHGASYSAAAVEAGAAAIVTDAAGAEIVAGLDLDVPVLVVPDPRAVLGTLSAHVLGVSREQAVKGLKAVQVPGRMEVIGASGGYVGMVDYAHTPDALERVLLALRQVMPDKSRLVTVFGCGGDRDRHKRPEMGRIASKLSDNVVLTSDNPRSEHPECILDEIAAGIPGGNVFWRFRDRRDAISCGVGLLSRGDVLLVAGKGHETYQEIKGVRYPMDERVLIREILSEKE"
  },
  {
    "reference": "MLREKTIILGVSGGIAVYKAAELLRLYVKAGAKVHVIMTRAAQEFVTPLTFQTLSGNPVHSELFNLIQEQEIGHISLADRADLIVVAPATANVIGKVANGIADDLLTTTLMASRAPVLFVPAMNVNMWENPLYRQNQEKLEQHGYRFVEPATGFLACGWEGKGKLPDPQTIFEESLALLFAGDLAGETVLVTAGPTREELDPVRYLSNYSSGKMGYALARAARARGARVILVSGPTALIPPAGVETIQVKSAVDMHQTVMKRAAEATIVIKAAAVADYRPVARAAAKVKKQGAGLTLELVPNPDILADLGRLPASPLLVGFAAETEALAANAAKKLAAKNLDLIVANDVTAPGAGFDADTNIVRLIYRDGREEALPQEQKERLAHQLLDRIIALRRDAGVG",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. We need a protein that can perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2 with high efficiency.2. The protein contains novel Flavoprotein domains that confer a unique function or activity.3. A protein that can modulate the overall flux of metabolites through the CoA from (R)- pantothenate: step 3/5 pathway, enhancing or suppressing its activity as needed.4. The protein must have high specificity towards phosphopantothenoylcysteine decarboxylase activity. The designed protein sequence is ",
    "response#1": "MNIVLGVGGGIAAYKSALLLRLFTEAGHKVTVIPTEASTKFVGVATWEALSGRPATNDVFDAVDQVNHVRIGHEADLIVVAPATADLLAKAAGGQANDLLTTTLLMARGPVLFAPAMHTEMWRHAATQANVATLRSRGIHVLDPAVGRLTGPDTGPGRLPEPEAIFAAAALLTQLEGAP",
    "response#2": "MNIVLGVGGGIAAYKSALLLRLFTEAGHKVTVIPTEASTKFVGVATWEALSGRPATNDVFDAVDQVNHVRIGHEADLIVVAPATADLLAKAAGGQANDLLTTTLLMARGPVLFAPAMHTEMWRHAATQANVATLRSRGIHVLDPAVGRLTGPDTGPGRLPEPEAIFAAAAVAAAMLAG",
    "response#3": "MTVIVGITGGIAAYKAVGVVRDLVKRGHDVHVVPTEGALRFVGLPTLEALSRNPVTTSVWDDVAEVRHVALGRRADLVVIAPATADSLARMAHGLASDLLGTTLLATEAPVVVAPAMHPQMWEHPATRANVATLRDRGVHLVGPVVGALTGDDAGIGRMAEPEDIVAAALDALGVPGALLAGRRVLVTAGGTHEPIDPVRFLGNRSSGKMGYALAIEARNRGASVILVSGPVALPPPYGVEMQQVETAIQMRNAVHRFITDVDVLVMSAAVADFRPDEVATQKIKKHSDEVQTHDGKLSLHLVPNPDILGELADTRNGSLEDSGRRLIRVGFAAETQQVVDYAQSKLHSKGLDLLVANDVSRSDSGFGTETNKVLLAGRTAVVTSGPTHEPSDPVRYIANRSXXXALARLGADVTLVSGPVTLPDPRGVKVIRVERAEEMLAAVLGALPADIAVMVAAVADWRVARENTEKIKKKPGEGPPPLMLAENPDILKTVGHHEKRPKVVVGFAAETQDVENNGRVKLRKKGADLIVANDVSPETGIMGGTRNSVKLITDQGLEPLPVMPKTGVAAAIWDRVAALLA"
  },
  {
    "reference": "MMATSKRGTWATIRRIAASDRISGLIMLGFALAGLLLANLPFTAHAFEQLENFHIAIPHTNIDMGLGHWVQDGLLTVFFLTVGLELKQELTTGSLSNPKAAAVPMLCAVGGMLAPPVLFIGVISLFAAAGSGQLVIASGTSFSFAQMSNGWAVPTATDIAFSLAVLALFAKALPGSIRAFLMTLATVDDLLAIILIAVFFSSVNAWYWFLGIAVCAVVWYFLVRMRKVPWLAVAIVGVLAWVMMFEAGVHPTLAGVLVGLLTPAHERFGEKTPRAERYADKLQPFSALLALPIFALFATGVHFESLTLALFVSPVVVAVMVALVVGKPLGIMTTAWLSTHVGGLKMAKGLRVRDMFPAACACGIGFTVSFLIASLAYQDAELSAEARFGVLVGSMVAAVISGILLSRQSKRFELEDGTKHKHHTGDEDAMNEVETVLEDGTVVVSQIIGTHQEQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons..2. The protein should contain a Helical membrane-spanning region.3. The protein can be expressed in plasma membrane and perform antiporter activity, sodium ion transmembrane transporter activity without triggering undesirable cellular responses.4. We are looking for a protein with broad substrate specificity for 2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out). The designed protein sequence is ",
    "response#1": "SCCKK",
    "response#2": "GLLSKIF",
    "response#3": "MXSXGXVXF"
  },
  {
    "reference": "MKKLLVIDFREKIKYEDAWDLQKKLHDLRVKDTIYDTLILLEHFPVITLGKFGDEKNLLKTKEELKSMGIDFIRVDRGGDITYHGPSQLVGYFIFKVDGVKNFVLKVEKSIINVLHEYGIEAKESIEYPGVWVEDRKITAIGIAIKKRVSYHGFALNVNNDLAPFSYIIPCGLKDKKVTSILKEANFSPPMEDVKRKVCLSVVKEFGFDSFMVFNFSSCKELSNFGMLLEDL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A key design requirement for the protein is the optimization of the Acyl-thioester intermediate to achieve high catalytic efficiency for L-lysyl-[protein] + octanoyl-[ACP] = H(+) + holo-[ACP] + N(6)-   octanoyl-L-lysyl-[protein].2. The protein should be designed to localize specifically to cytoplasm in order to perform its role in protein lipoylation more effectively.3. The protein must exhibit the following characteristics: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate- dependent enzymes; Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate..4. The designed protein should have a high specificity for substrate binding, with minimal off-target effects.5. A protein that can modulate the overall flux of metabolites through the protein lipoylation via endogenous pathway, Protein modification, protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 1/2 pathway, enhancing or suppressing its activity as needed.6. The presence of BPL/LPL catalytic domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MARMRVMVRERIDGVRPDTLWFLSHPPVYTVGKRTPPEHRPLAGLGIPVHETNRGGLLTYHAPGQLVGYVMCHIGAMNAVVPFLRLLEARLVDTVEALGIPAERRDTPPGSVELTGVWTRRTNRKIASIGLRCTRKVTSHGFALNVDCDMRPWTWATPCGMPEVEMTSVQRELTDAGHAVPSMAEVRELVALLDGEAGELVWLVEHPPLYTAGTSAKAADLLEARFPVHAVGRGGQYTYHGPGQRVVYVMLDLNRRGRDVRAFVKALENWVIDALAEFNLRGEIRDGRVGVWIARPDKAPLPDGSMREDKIAAIGVKLRRWVSFHGIAINVEPDLNHYAGIVPCGISGHGVTSLVDMGLPVGMDDLDLALRRSFARNFPPLWPRPTWPHLPVGSRGNTVLMVEHDPVYTAGRRTEKHEYPWDGTEVVPIGRGGKLTYHGPGMLVGYPIVRLPIPLDVVRFVRVLEEVIIAVVRDFGVPATTVEGRSGAWVLADERGPDRKISAIGVQVSKRATMHGFALNCSNDLRPFGKIIPCGITDAGVTSLSAETGNRIDPADVVERMEQELSAREADLARLVGEPPVRVGEAGELVWLVEHPPLYTAGTSAKAADLLEARFPVHAVGRGGQYTYHGPGQRVVYVMLDLNRRGRDVRAFVKALENWVIDALAEFNLRGEIRDGRVGVWIARPDKAPLPDGSMREDKIAAIGVKLRRWVSFHGIAINVEPDLNHYAGIVPCGISGHGVTSLVDMGLPVGMDDLDLALRRSFARNFPPL",
    "response#2": "MARMRVMVRERIDGVRPDTLWFLSHPPVYTVGKRTPPEHRPLAGLGIPVHETNRGGLLTYHAPGQLVGYVMCHIGAMNAVVPFLRLLEARLVDTVEALGIPAERRDTPPGSVELTGVWTRRTNRKIASIGLRCTRKVTSHGFALNVDCDMRPWTWATPCGMPEVEMTSVQRELTDAGHAVPSMAEVRELIRLRAPRVDYQEALAFMEARAAAIAAGEAGELVWLVEHPPLYTAGTSAKAADLLEARFPVHAVGRGGQYTYHGPGQRVVYVMLDLNRRGRDVRAFVKALENWVIDALAEFNLRGEIRDGRVGVWIARPDKAPLPDGSMREDKIAAIGVKLRRWVSFHGIAINVEPDLNHYAGIVPCGISGHGVTSLVDMGLPVGMDDLDLALRRSYAQGLELQRQLVEARHSGRIGNTLLLLEHPPVLTLGRNSERKNVLASDEFLAYRGVEIHEVNRGGDVTYHGPGQLVGYPILDLRSFAESGERGRLGAVEYVRWVEEALIRTCADFGVQTQRVAGRTGVWTLPGGSVEEKKIAAIGVHISRGITSHGFALNVTTDLRDFDLIVPCGISDRKVTSLELEVIDEPALTMEKVIHSAARQLREEGRIEAYEQSWQAMTAFTNQRTPDTPDQLWLVEHPPVFTQGQAGKAEHLLFPGDIPVVQTDRGGQVTYHGPGQLVAYPLLDLRRLKMGVRDLVTAIEQTIVATLAEYGIESYPKPDAPGVYVENHKIASLGLRVRRGCSFHGLALNVDMDLSPFLRINPCGYQGLAMTQMRDLMPETPSLVQVQEQLVCQF",
    "response#3": "MARMRVMVRERIDGVRPDTLWFLSHPPVYTVGKRTPPEHRPLAGLGIPVHETNRGGLLTYHAPGQLVGYVMCHIGAMNAVVPFLRLLEARLVDTVEALGIPAERRDTPPGSVELTGVWTRRTNRKIASIGLRCTRKVTSHGFALNVDCDMRPWTWATPCGMPEVEMTSVQRELTDAGHAVPSMAEVREAVRILAAGGEPGTSPETLLLLEHPPVYTIGRGGCRENVLDPGIRVVEANRGGDVTFHGPGQLVGYPVIDLGRRGRDLHRYLRILEELLINVAAEFGVAARREPGRTGIWTDGGKLASIGVGVRRWVTMHGFALNVTNELAPFRSINPCGIVACPITSLSAILGRPVPLGDVRRSVAGRFPFLLDERDYQEALAFMEARAAAIAAGEAGELVWLVEHPPLYTAGTSAKAADLLEARFPVHAVGRGGQYTYHGPGQRVVYVMLDLNRRGRDVRAFVKALENWVIDALAEFNLRGEIRDGRVGVWIARPDKAPLPDGSMREDKIAAIGVKLRRWVSFHGIAINVEPDLNHYAGIVPCGISGHGVTSLVDMGLPVGMDDLDLALRRSLREPADADLAALAIQEQLAAGVAAGTSPETLLLLEHPPVYTIGRGGCRENVLDPGIRVVEANRGGDVTFHGPGQLVGYPVIDLGRRGRDLHRYLRILEELLINVAAEFGVAARREPGRTGIWTDGGKLASIGVGVRRWVTMHGFALNVTNELAPFRSINPCGIVACPITSLSAILGRPVPLGDVRRSVAGR"
  },
  {
    "reference": "MSLRSQYNSNSSSSNTNPAQQQPHQQHKRKCRSTTTTTTSCYTCLRSQRPAMIALVIVVAAFVCLPAQFVGALQAVSSSSSSSRYDNYLGDNRKPAFKNCAGYAPSVKEEQPENTYVFTVQATDPDPNQEITYSLVQSAFDRAKFVIHPTSGVIVTAHIFDRDEPIHEKFVFVTVQATDNGLPPLDDVCTFNVTIEDINDNPPVFNKARYDESMSENTMIDAVVMSISASDFDDRNNSIVEYEILRERDFQYFRIDRESGIIYLSRPIDRRPGQTYTINVRAFNIVPDPLQEAHIEVRIRVVESSIKPPSFVDPIDEPIYLKENLKNFSQPIATLRAVSNMPDKPEVIFELITGRTEQTNGKNTFVFNQIGNEVTIKLGKSLDYEAITEYTLTMSVRNTFELAAEHQIKIKVEDVNDNIPFFTEVKSGTILENEPPGTPVMQVRAFDMDGTPANNIITFELVDNREIFAIDPNSGNITALTTFDREERDFYNVKVSATDNSPSSLFDNGDHNHGYQVFRISIGDKNDHKPHFQKMTYLADKLLEDANTNYEVIEVKAEDEDNASQIVYSIESGNVGDAFKIGLKTGKITVNQRLDYETVTEYELKVRAFDGVYDDFTTVVVKIEDVNDNPPVFKEEYSKTILEGPVLSNDCILNIEAYDPDIKDRNADQHITYSIVKEDHRKLLTIDNNGCLRLIQPLDRDPPNGHKSWQVLISASDEDGVGTSLKSVKPVIITLEDINDNAPFLINQMPVIWQENRNPGQVVQLLAN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must have at least one Cadherin, EGF-like domain.2. The designed protein should have myosin binding, protein homodimerization activity within cell cortex, filopodium to regulate maintenance of epithelial integrity, open tracheal system, asymmetric cell division, female germ-line stem cell population maintenance. The designed protein sequence is ",
    "response#1": "SGVFELKLQEFVNKKGLLGNRNCCRGGAGPPPCACRTFFRVCLKHYQASVSPEPPCTYGSAVTPVLGVDSFSLPDGGGADSAFSNPIRFPFGFTWPGTFSLIIEALHTDSPDDLATENPERLISRLATQRHLTVGEEWSQDLHSSGRTDLKYSYRA",
    "response#2": "KSTTCEVLKYNTCLGSPLPYTHTSLVLAEDSSTQEEAFEKLAMWSGLRNAPRCWAVIQPLLCAVYMPKCENGRVELPSQHLCQATRRPCSIVDQERGWPNFLKCEETNKFPIGCTNEVQKLKFNTSGRCEAQ",
    "response#3": "MHAYIAMFGTVTLLCTFFTLATFLADWKNSNRYPAVILFYVNACFFMGSIGWLAQFMDGARKEIVCKSDNTMRLGEPSSTETLSCVIIFSLVYYSLMSGVIWFVMLTYAWHTSFKALGTTHQPLSGKTSYFHMVTWSIPFILTVSILAIAEVDGDSVSGICFVGYKNYRYRAGFVLAPIGVVLVVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGIFGFLAFGFVFITFGCHFYDFFNQAEWEKSFREYVLCEAC"
  },
  {
    "reference": "MSGNIGANPIGKKVNISCSLDEAIDELLMESALKSGWSKKREAELRLEDHLRRFSLVPAEEQYIEKKVD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTCGL",
    "response#2": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC",
    "response#3": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC"
  },
  {
    "reference": "MNIIDRFEQENISKRTANKKIPDFEAGDTVKVTVKIIDRSIEKDGKEKLTERFQAYEGVVIAKRNRGITSSFLVRKISHGEGVERRFMTYSPIVHSIDVVKYGVVCRAKLYYLRNRSGKSARIKERHIPIAKTKAAKA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that contributes to translation by functioning in ribosome, ribonucleoprotein complex with structural constituent of ribosome.2. The protein must exhibit the following characteristics: This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site.. The designed protein sequence is ",
    "response#1": "PTVRGSVMNPNW",
    "response#2": "PTVRGSVMNPNG",
    "response#3": "PTVRGSVMNPNW"
  },
  {
    "reference": "MSERFLPEKDAYRVAVVGATGAVGRTMVEILEQRGFPVEELIPLASSRSAGSKIEFGGKEYVVRDLEQFDPAGLDIALFSAGGGTSLAHAPRFAEAGCYVIDNSSAWRMEPDVALIVPEVNPDALEMARHNRIIANPNCSTIQMVVVLKPLHEVARIKRVVVSTYQAVSGAGGKGMDELARQTAQLLNAREVEQEVFPARIAFNVIPHIDVFLEDGYTKEERKMMDETRKILDPSIAVTATTVRVPVFYGHSESVNVTFERPLPAEEARAILADSEGICVVDDPASEDYPMPSEAAGSDPVWVGRIRDDTSFPNSLQLWIVADNVRKGAALNAVQIAEMLIR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Ideal protein should have a binding affinity for key enzymes in the L-threonine from L-aspartate: step 2/5 pathway to enhance the overall efficiency of the pathway.2. The designed protein should be able to compete with endogenous proteins or inhibitors for phosphate binding.3. The designed protein should have aspartate-semialdehyde dehydrogenase activity, NAD binding to facilitate lysine biosynthetic process via diaminopimelate, diaminopimelate biosynthetic process, 'de novo' L-methionine biosynthetic process.4. The protein should be designed to have high stability and activity towards L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH. The designed protein sequence is ",
    "response#1": "MVYALVGATGAVGGVMIDIMNARQDWPWGEVRLVASPRSAGKKMTVRGEELTVLALAPEVFDGVDIAMFDVPDEVSARWAPIAAERGAVAVDNSGAFRMDPDVPLVVPEVNADQISLKPDAKGRLIGGPLKPLMKFGIEKIYLASMQAISGAGYDGVASMAILDNVIPYIDKEEEKVETETKKLLGEFNGSEIVDAPFAVSASCNRVMVMDGHTEAVWVEMADNPSTEDIKQAFLEFDPGLSDLPTEPAPVIEVKEEKDRPQPRMDRNLGGGMTVSVGRIRPGIRYICMGHNP",
    "response#2": "LAVVGATGAVGTVMLDLLSTREDVWGEIRLIASPRSAGRRLTVRGEQVEVIALSEEAFDGVDVAMFDVPDEVSAAWVPVAAARGAVCVDNSGAFRMDPEVPLVVPEVNPDDLAALHKAFKVKRVVVTTYQSVSGAGKEAMDELFDQTRGIYMNQPIYETQKKFPKQIAFNVIPQIDVFMDDGSTKEEWKMMVETKKILDPKIKVHANCARVPVFIGHAEYVNIETEKEISDEAATKVLKHTKGVEVIDLREPDGYPTQAEIAGEDDVFVSRIRQDFSVENGLSFWCVADNLRKGVIRVNPTCVRVPVFFGHLKPIYDAVGIDRINVSTYQSVSGAGKEAIEELAKQCADLLSGKPVKVENFSRQIAFNSIPQIDKFMDNGYTKEEMKMVWETKKILGDENVLVNPTAVRVPAFYGHGEAIHIETRQQTSAEEVKEILANAPGVVVAHNDEDFPTQVSDASGKDDTFVGRIREDISHPNGINMWVVADNVRASAEAILGALHHEYGLRELVVASYQAVSGAGQVGIDRLHAELAAVAGKQVGARAGDVREALAAAGLSQEDSPFSGPVVLNVVPWAGSLKDDGWSSEELKVRNESRKILGIPDLKVSATCVRVPVVTTHALAVHATFERPVTVAEAHKIFDAQPTVVLVDDASDGRYPMPADVVGEDPTYVGRVRQALDFPNTLDFFVCGDNLRDAAEIGRASARVLALVGATGAVGGVMIDIMNARQDWPWGEVRLVASPRSAGKKMTVRGEELTVLALAPEVFDGVDIAMFDVPDEVSARWAPIAAERGAVAVDNSGAFRMDPDVPLVVPEVNADQIAS",
    "response#3": "MLSGLFPLAKRYGLKRVVVTTFQSVSGSGKQAVEQLRAERKGELNTGSNVYPHPIFNNCLPHIGNFLENGCTDEEIKMVNESRKILDLPDLPITATTVRVPVEYSHSVSLNVELEANFNIEDVRNLYRHTAGIILKDAPAQNIYPLPRDAMGRDDVFIGRLRRDESVPFGLNMWVVADNLRSRVAHLFVGLKPIYDAVGITRINVATYQAVSGTGKDAIEELAGQTARLLNGQPAETKVYPKQIAFNVLPHIDVFLDNGYTKEEMKMVWETQKIFADANIQVNPTCVRVPVFFGHSEAVHIETEQKISAEQARELLENAPGIVVIDDHADGGYPTPVSDGAGTDPTYVGRIREDISHPNGLDMWIVSDNVRLKPLMKFGIEKIYLASMQAISGAGYDGVASMAILDNVIPYIDKEEEKVETETKKLLGEFNGSEIVDAPFAVSASCNRVMVMDGHTEAVWVEMADNPSTEDIKQAFLEFDPGLSDLPTEPAPVIEVKEEKDRPQPRMDRNLGGGMTVSVGRIRPGIRYICMGHNSR"
  },
  {
    "reference": "MAGHSKWANIKRQKARVDAQKGKIFARLSRAIIIAARHGGGDPAGNFQLRSAIEKAKAAGIPSENIERAIAKGTGTLDSDAPLEAIRYEGYGPGGVAFLIEALTDNRNRTAADLRAAFNKQGGNLGETGCVGWMFEQWGIVTVTAPRDEEAFLEALLAADVETYEILEEVAEVRCPVPALETVSETLKEHGYTVLDTESRWIPMNTVEITDEDTARRVLKLMDALENLDDIQSVATNVTMSDALMEAMYV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in regulation of DNA-templated transcription. The designed protein sequence is ",
    "response#1": "MLPLVCKGEGSLLFEATSLLLWGHTGLSLELRARTVVEMLLHRHYYLQGMIDSKVMLQAVRYSLCSEESPEMTNLSFATLEAIFDADVKATCFPTSFSNVWHLYALASILECNIYSIYPMRNIKIRPYFNRVIMPRCSTHVTSMLHIMWAGQPLTSHLFRHQYFAPVR",
    "response#2": "MLPLVCKGEGSLLFEATSLLLWGHTGLSLELRARTVVEMLLHRHYYLQGMIDSKVMLQAVRYSLCSEESPEMTNLSFATLEAIFDADVKATCFPTSFSNVWHLYALASILECNIYSIYPMRNIKIRPYFNRVIMPRCSTHVTSMLHIMWAGQPLTSHLFRHQYFAPVR",
    "response#3": "MNDKANDLVQLAISVTEEMLPIHQDELLAHTGKEFEDVFPNILNRATLILDMFYGLSLIEVDTSEHIYLLVQQPESEEEQVMLESLGRPTQEYVMPILGLIFLMGNRVKEANVWNLLRRFSVDVGRKHSITRKLMRQRYLECRPLSYSNPVEYELLWGPRAHHETIKMKVLEYMARLYRKRPQNWPEQYREC"
  },
  {
    "reference": "MATKMAGVKRAGRKRKERKNIERGAAHIRSTFNNTIVTITDVEGNTISWSSAGTLGFRGSRKSTPFAAQMAAEAAAKAAMEHGLKTVEVYVKGPGSGREAAIRALQAAGLEVSLIKDVTPIPHNGCRPPKRRRV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Forms part of the Shine- Dalgarno cleft in the 70S ribosome.; Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA2. The designed protein should be involved in translation by localizing to ribosome and exhibiting structural constituent of ribosome, rRNA binding. The designed protein sequence is ",
    "response#1": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#3": "MIPIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG"
  },
  {
    "reference": "MLDKVKEIIVEQLGVDADQIKPESNFVDDLGADSLDTVELIMSFEEEFGVEIPDTEAEKIKTVQDVINYIEANKK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must be able to perform acyl binding, acyl carrier activity, phosphopantetheine binding under specific conditions.2. A protein that can modulate the overall flux of metabolites through the fatty acid biosynthesis, Lipid metabolism pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "VFSGMGSQWTEMGSSLMAIPKFRESIERCQKVLESKGLNLIEILTSTDATIFDNILHSFVGIAAIQIGLVDLLRSLNIEPDYIIGHSVGELGCGYADDAFTPEQMILAAYYRGKVSLDIEKIKGSMAAIGMGYKKIVNMLPEKIEVACHNSAESCTISGPAEDVEKFVNELKSRGIFAKEVPCSNIAYHSRYIAHMGPQLLKYLKKIIPNPKPRSSKWLSSSVPESDWHQEGRNLCSAEYHTNNLLNSVLFEETFALLPNNALTIEIAPHGLLQAILKRSMVNGVHIPLTQRSP",
    "response#2": "VFSGMGSQWTEMGSSLMAIPKFRESIERCQKVLESKGLNLIEILTSTDATIFDNILHSFVGIAAIQIGLVDLLRSLNIEPDYIIGHSVGELGCGYADDAFTPEQMILAAYYRGKVSLDIEKIKGSMAAIGMGYKKIVNMLPEKIEVACHNSAESCTISGPAEDVEKFVNELKSRGIFAKEVPCSNIAYHSRYIAHMGPQLLKYLKKIIPNPKPRSSKWLSSSVPESDWHQEGRNLCSAEYHTNNLLNSVLFEETFALLPNNALTIEIAPHGLLQAILKRSMVNGVHIPLTQRSYILVAPHRTWWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINVLKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGR",
    "response#3": "VFSGMGSQWTEMGSSLMAIPKFRESIERCQKVLESKGLNLIEILTSTDATIFDNILHSFVGIAAIQIGLVDLLRSLNIEPDYIIGHSVGELGCGYADDAFTPEQMILAAYYRGKVSLDIEKIKGSMAAIGMGYKKIVNMLPEKIEVACHNSAESCTISGPAEDVEKFVNELKSRGIFAKEVPCSNIAYHSRYIAHMGPQLLKYLKKIIPNPKPRSSKWLSSSVPESDWHQEGRNLCSAEYHTNNLLNSVLFEETFALLPNNALTIEIAPHGLLQAILKRSMVNGVHIPLTQRSFAAAAE"
  },
  {
    "reference": "MRVKEIRKSCLWKWGTMLLGMLMICSATGQLWVTVYYGVPVWKEATTTLFCASDAKAYKTEVHNVWATHACVPTDPDPQEVKLENVTENFNMWKNNMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCSNVNITSNVTNATSNATATSNATSDNGREEMKNCSFNTTTGVKDKLRKDYALFYSLDIVPIDNDNRNNSYRLISCNTSIITQACPKVSFEPIPIHYCAPAGFAILKCNDKTFNGSGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSENFTNNAKTIIVQLNETVEINCTRPNNNTRRSIPIGPGRAFYGMGDIIGDIRQAHCNISGTKWNNTLKRVVAKLREQYGNNKTIQFTNHSGGDPEIVMHSFNCRGEFFYCNTDKLFNSNWTTNTSSENVTSGTEGNDIITLPCRIRQIINLWQEVGKAMYAPPIQGQINCSSNITGLLLTRDGGANQTNNTEVFRPGGGDMRDNWRSELYKYKVIKIEPVGIAPTKAKRRVVQREKRAVTIGAVFLGFLGTAGSTMGVASLTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTTVPWNTSWSNKSWEQIWHNMTWMEWEKEIDNYTEIIYSLLEESQNQQEKNEQDLLALDKWANLWNWFNITNWLWYIKIFIIIVGGLVGLRIVFAVLSIINRVRQGYSPLSFQTRFPAPRGPDRPEGTEEEGGERDRDRSIRLVNGFLALFWEDLR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein's functional design necessitates the inclusion of a YXXL motif; contains endocytosis signal motif.2. The protein should be optimized for stability and solubility when expressed in virion membrane, plasma membrane, host cell endosome membrane where it will be involved in positive regulation of receptor clustering.3. The protein must exhibit the following characteristics: The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes. The designed protein sequence is ",
    "response#1": "MGVASMTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGLWGCSGKLICTTAVPWNSSWSNKSYADIWSNMTWMQWDREINNYTNTIYRLLEESQSQQEKNEKDLLALDSWQNLWNWFDITHWLWYIKIFIMIIGGLIGLRIVFAVLSLVNRVRQGYSPLSFQTY",
    "response#2": "EMKNCSFYITTEIRDRRKQEYALFYRSDVVQINGNNISYRLINCNTSAIKQACPKVNFEPIPIHYCAPAGYAILKCNGENFNGTGLCENVSTVQCTHGIRPVVSTQLLLNGSLAEKEIVIRSENITNNAKTIIVQLNETVIINCTRPSNNTRKSIRIGPGQTFYATGDITGDIRQAHCNISATKWINTLQQVANKLRDHFNKTIEFQPSSGGDPEITEHTFNCGGEFFYCNTTNLFNWTFNSISNNINTSTNEITIQCRIKQIINRWQGVGKAMYAPPTAGSINCLSNITGLLLTRDGGNTSEEIFRPGGGDMRDNWRSELYKYKVVRLEPLGLAPTKARRRVVEREKR",
    "response#3": "MKEEIKNCSFNITTELRDKKKKESALFYRIDIVPLNEKSNNSNGSSEYRLINCNTSTITQACPKVSFDPIPIHYCAPAGYAILKCNDKTFNGIGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENLTNNAKTIIVHLNKSIAIMCTRPGNNTRKSVRIGPGQTFYATGGVIGDIRQAFCIINGSNWNKTLQEVEEKLKRHFPNATEIKFEPSSGGDLEVTTHSFNCRGEFFYCDTTILFNRTMITDNINTLPCRIKQIINMWQGVGRAMYASPIAGNITCISNITGLLLTRDGGKTNSTTETFRPGGGNMKDNWRSELYKYKVVEIKPLGIAPTGAKRRVVEREKR"
  },
  {
    "reference": "MGFSLTDYAPYDFANRRHIGPSPKEMGQMLATLGVPSLEALINEALPEGIRRRDPLAFGPALSERDTLHRMRELADKNTVLTSLIGQGYHGTHTPPVILRNILENPAWYTAYTPYQPEISQGRLEALLNFQTMMADLTGLPIANASLLDEGTAAAEAMAMAQRASKSKARGFFVAEDCHPQTIDVIRTRAEPLGIEVIVGAVDALDPEAVFAALFQYPGSYGHVRDYSDVIEALHGARALAVVAADPLALTLLKSPGEMGADIAIGSTQRFGVPMGYGGPHAAYMTCTDALKRSMPGRIIGVSVDARGNKAYRLSLQTREQHIRREKANSNVCTAQALLAVMASMYGVFHGPDGLKAIAQTVHRKTARMADGLTELGFKVDPQDYFDTITVKVGSMQGVILAAALREGVNLRKVGTDRIGITLDELTLGRTIEAVWRAFGAEGMVYDKTRMVYHLPQEMLRESSYMTHPIFHMNRAEAEMTRYMRRLADRDLALDRAMIPLGSCTMKLNATVEMLPLTWPEFANLHPFAPADQAAGYHEMIAELSQMLCDVTGYDAMSMQPNSGAQGEYAGLLAIRGYHRARGEGHRNICLIPTSAHGTNPASAQMVGWKVVVVKSAENGDIDLEDFRAKAEQHSENLAGCMITYPSTHGVFEEIVREVCDITHAHGGQVYIDGANMNAMVGLSAPGDLGGDVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPHLPSHATATGAGFGDAGAVASAAYGSPSILTISYAYCLLMGGAG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of pyridoxal 5'-phosphate is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "AGRPTPLTLCKNITKNTNTKLYLKREDLLHGGAHKTNQVLGQALLAKKMSKKEIIAETGAGQHGVATALSCALLGLKCRIYMGFKDVIRQQQNVFRMQLMGAKVISVKNGSGTLKDACNEALRDWSENYCTSHYMIGTAAGPHPYPTMVKKFQSIIGIETRCQILKQESCLPNIIIACIGGGSNAIGIFSEFIKDKSVCLIGVEPAGKGIKTGKHGAPMQHGSTGIYFGMKAPVMQTSEGQIKESWSISAGLDFPAVGPEHAWLKHIGRATYVSITDCEAINAFKILCKLEGIIPALESSHALAYALKLISKDTNKKQIIIVNIG",
    "response#2": "AGRPTPLTLCKNITKNTNTKLYLKREDLLHGGAHKTNQVLGQALLAKKMSKKEIIAETGAGQHGVATALSCALLGLKCRIYMGFKDVIRQQQNVFRMQLMGAKVISVKNGSGTLKDACNEALRDWSENYCTSHYMIGTAAGPHPYPTMVKKFQSIIGIETRCQILKQESCLPNIIIACIGGGSNAIGIFSEFIKDKSVCLIGVEPAGKGIKTGKHGAPMQHGSTGIYFGMKAPVMQTSEGQIKESWSISAGLDFPAVGPEHAWLKHIGRATYVSITDCEAINAFKILCKLEGIIPALESSHALAYALKLISKDTNKKQIIIVNIH",
    "response#3": "AGRPTPLTLCKNITKNTNTKLYLKREDLLHGGAHKTNQVLGQALLAKKMSKKEIIAETGAGQHGVATALSCALLGLKCRIYMGFKDVIRQQQNVFRMQLMGAKVISVKNGSGTLKDACNEALRDWSENYCTSHYMIGTAAGPHPYPTMVKKFQSIIGIETRCQILKQESCLPNIIIACIGGGSNAIGIFSEFIKDKSVCLIGVEPAGKGIKTGKHGAPMQHGSTGIYFGMKAPVMQTSEGQIKESWSISAGLDFPAVGPEHAWLKHIGRATYVSITDCEAINAFKILCKLEGIIPALESSHALAYALKLISKDTNKKQIIIVNIG"
  },
  {
    "reference": "MRVRGIQKNYPPLWKWGMIFLGLIIICKATDKLWVTVYYGVPVWKDAETTLFCASDAKAYSQEVHNVWATHACVPTDPDPKELXLDNVTEKFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLTCSNFTSNFTTANATDADMKNCSYNVTTELIDKQQKVHSLFYKLDIEQSGENSSEYRLINCNTSAIAQACPKISFEPIPIHYCAPAGFAILKCNEENFNGTGPCTNVSTVQCTHGIMPVVSTQLLLNGTRAEGEVMIRSENLTDNAKTIIVQLNETVWINCTRLNNNTRKSVRIGPGQAFYVTGDIIGDIRQAHCNVSKIKWKNALKKVQEKLKRHFNKTTIEFKSSSGGDLEVTTHSFNCMGEFFYCNTSALFNTSLLNDTENGTNDNITLPCRIKQIVXMXQRVGQXMYAPPIAGIIQCNSTITGLLLTRDGGKDDTNNTETFRPEGGNMKDNWRSELYKYKVVKIEPLGVAPTRAKRRVVEREKRAVGLGAVFFGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQNNLLRAIEAQQHMLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNKSQEEIWGNMTWLQWDKEISNYTHKIYELIEESQNQQEKNEQDLLALDKWANLWTWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLTVINRVRQGYSPLSFQTLTHQQREPDRPGKIEEGGGEQDRDRSVRLVNGFLALAWDDLRSLCLFSYHRLRDFALIAARTVE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The protein's functional design necessitates the inclusion of a YXXL motif; contains endocytosis signal motif.3. The protein contains novel Human immunodeficiency virus 1 envelope glycoprotein Gp120, Retroviral envelope protein GP41-like domains that confer a unique function or activity.4. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.5. For general function, the protein need meet that During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Transmembrane protein gp41: Acts as a class I viral fusion protein; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane The designed protein sequence is ",
    "response#1": "MRVKETQMNWPNLWKWGTLILGLVIICSASENLWVTVYYGVPVWKEANTTLFCASDARGYDTEVHNVWATHACVPTDPNPQEVVLENVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNLRNDTNSVDINEEIKNCSFYATPELKDKRKKENALFHSLDIVSFVEKNFTKYKLIHCNTSTIAQACPKVSFDPIPIHYCAPAGYAILKCNDNTFNGTGPCSNVSTVQCTHGIKPVVSTQLLLNGSLAEEGIIIRSENLTNNIKTIIVHLNESVEIVCTRPNNNTRKSVRIGPGQAFYATKGITGDIRQAHCNISEKAWNKTLQEVSKKLRGHFPSNTAIKFEPSSGGDLEITTHSFNCRGEFFYCNTTKLFNRTYLTNETYEFENASDSNLTLQCRIKQIINMWQGVGRAMYAPPIEGNITCKSNITGLLLTRDGGNDNSGTETFRPGGGDMRNNWRSELYKYKVVEIKPLGLAPTKAKRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASVTLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLALERYLRDQQLLGIWGCSGKLICTTNVPWNSSWSNRSQNEIWDKMTWIQWEREIDNYTQGIYNLLEESQNQQEKNEKELLELDKWASLWNWFDITNWLWYIKIFIMIVGGLIGLKIIFTVLSIVNRVRQGYSPLSFQTHLPAQRGPDRPEGIEEE",
    "response#2": "LEMKNCSFNMTTEIRDKKQKVFSLFYKLDIVKINGNNNSNSSGEYRLINCNTSAITQACPKVSFQPIPIHYCAPAGFAILKCKDEEFNGTGPCSNVSIVQCTHGIRPVVSTQLLLNGSLAKGEVKIRSENISDNAKTIIVQFNQSVAINCTRPNNNTRRGIGIGPGQTFYATDDIIGDIRQAHCNVSRAQWNNTLRQVVTQLKKYWNKTITFANSSGGDLEITTHSFNCRGEFFYCNTSDLFNNIWIGNETTETNETETNSSKPITLQCRIKQIINMWQRVGQAIYAPPIQGKLECNSNITGLLLTRDGGKNNDSKEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRAQRRVVKREKRXLGAAXVFLGFLGAAGSTMGAASVTLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTAVPWNTSWSNKSMDEIWNNMTWMEWEKEIDNYTHIIYNLIEDSQNQQEKNEQDLLALDKWANLWNWFSITNWLWYIKIFIMIVGGLVGLRIIFTILSVVNRVRQGYSPLSLQTRLPAXRGPDRPEGTEEEGGERDRDGSGRLVDGFLALIWDDLRSLCLFSYHRLRDLL",
    "response#3": "MRVMGIQRNYQHWWRWGTMLLGLLMICNVAENLWVTVYYGVPVWKEANTTLFCASDARGYDTEVHNVWATHACVPTDPNPQEVVLENVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTNLRNDTNTFNNSKNDSLFEEMKNCSFNMTTELRDKRKTVHSLFYKLDIVSMNGNDSGQYRLINCNTSTMTQACPKVTFEPIPIYYCTPAGFAILKCRDPNFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEKKVMIRSENITDNSKTIIVQLTKPVNITCIRPGNNTRTSVRIGPGQTFYGTGDVIGDIRKAYCNVSKEAWTSTIQNISXQLREYFNNKTIIFKNSSGGDLEVTTHSFNCGGEFFYCNTAGLFNSTWNGTEINATGTNETIXLPCRIKQIINMWQRVGQAMYAPPIRGMIKCESNITGLLLTRDGGGRNNSTNETFRPIGGDMRDNWRSELYKYKVVQIEPIGVAPTRAKRRVVEREKRAVGIGAMFLGFLGAAGSTMGAASITLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLGDQQLLGIWGCSGKLICTTAVPWNASWSNKSLNDIWDNMTWMQWDREISNYTSKIYKLIEKSQNQQEKNEQELLELDKWASLWDWFSITNWLWYIRLFIMIVGGLVGLRIIFAVLSIVNRVRQGYSPLSFQTRLPAQRGPGRPEGI"
  },
  {
    "reference": "MDDPKKEDILLLADEKFDFDLSLSSSSANEDDEVFFGPFGHKERCIAASLELNNPVPEQPPLPTSESPFAWSPLAGEKFVEVYKEAHLLALHIESSSRNQAAQAAKPEDPRSQGVERFIQESKLKINLFEKEKEMKKSPTSLKRETYYLSDSPLLGPPVGEPRLLASSPALPSSGAQARLTRAPGPPHSAHALPRESCTAHAASQAATQRKPGTKLLLPRAASVRGRSIPGAAEKPKKEIPASPSRTKIPAEKESHRDVLPDKPAPGAVNVPAAGSHLGQGKRAIPVPNKLGLKKTLLKAPGSTSNLARKSSSGPVWSGASSACTSPAVGKAKSSEFASIPANSSRPLSNISKSGRMGPAMLRPALPAGPVGASSWQAKRVDVSELAAEQLTAPPSASPTQPQTPEGGGQWLNSSCAWSESSQLNKTRSIRRRDSCLNSKTKVMPTPTNQFKIPKFSIGDSPDSSTPKLSRAQRPQSCTSVGRVTVHSTPVRRSSGPAPQSLLSAWRVSALPTPASRRCSGLPPMTPKTMPRAVGSPLCVPARRRSSEPRKNSAMRTEPTRESNRKTDSRLVDVSPDRGSPPSRVPQALNFSPEESDSTFSKSTATEVAREEAKPGGDAAPSEALLVDIKLEPLAVTPDAASQPLIDLPLIDFCDTPEAHVAVGSESRPLIDLMTNTPDMNKNVAKPSPVVGQLIDLSSPLIQLSPEADKENVDSPLLKF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: May be involved in p53-induced cell cycle arrest in G2/M phase by interfering with microtubule rearrangements that are required to enter mitosis; Overexpression delays G2/M phase progression..2. The protein should be designed to localize specifically to membrane, nucleoplasm, cytoplasmic microtubule, cytosol, microtubule cytoskeleton in order to perform its role in DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest, microtubule-based process more effectively.3. Target a Basic and acidic residues, Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "IVISQMIDENKSRENRASLPLPCAIAQSRAHHAKQSLANRSGVNIHRAFALLPGRLGIPAPSDERGPEAELPPKEERPCGGPRRGFASITITARRVGPPARALVWGTAGDSLCPKCRAEDTLFQAPPALANGAHPGRHQRSFACTEFSRNSSVVRLKVPEAHTGLCERRKYWVTHADDKETSFSPDTPLSGKSPLVFSSCVHLRVSQQCPDSIYYVDKSLSVPIEPPQIASPKMHRSVLSLNLNCSSHRLTADGVDGLVNREPISEALKQELLEGDQDLVGQRWNPGLQESHLKETPSLRRVHLGTGACPWSGSFPLENTELANVGANQVTVRKGEKDHTTHCHASDHANQLSIHIPGWSYRAVHTKVFSGSSKRQQGEVCMTVSAPPVEQKPTRHFLPIGDSSPSDDCLSRDLSEPTERRHQSFLKPRILFPGFLCPLQDVCASLQEDNGVQIESKFPKGDYTCCDLVVKIKECKKSEDPTTPEPSPAAPSPAPRDGAGSPGLSEDCSESCPED",
    "response#2": "RPASLSNDKLMEKSEPVDQRRHTAGKAIVDSRSAQPKETSEERKARLSEWKAGKGRVLKRPPNSVVTQHEPAGQNEKPVGSFWTTMAEEDEQRLFTEKVNNTFSECLNLINEGCPKEDILVTLNDLIKNIPDAKKLVKYWICLALIEPITSPIENIIAIYEKAILAGAQPIEEMRHTIVDILTMKSQEKANLGENMEKSCASKEEVKEVSIEDTGVDVDPEKLEMESKLHRNLLFQDCEKEQDNKTKDPTHDVKTPNTETRTSCLIKYNVSTTPYLQSVKKKVQFDGTNSAFKELKFLTPVRRSRRLQEKTSKLPDMLKDHYPCVSSLEQLTELGRETDAFVCRPNAAC",
    "response#3": "RPASLSNDKLMEKSEPVDQRRHTAGKAIVDSRSAQPKETSEERKARLSEWKAGKGRVLKRPPNSVVTQHEPAGQNEKPVGSFWTTMAEEDEQRLFTEKVNNTFSECLNLINEGCPKEDILVTLNDLIKNIPDAKKLVKYWICLALIEPITSPIENIIAIYEKAILAGAQPIEEMRHTIVDILTMKSQEKANLGENMEKSCASKEEVKEVSIEDTGVDVDPEKLEMESKLHRNLLFQDCEKEQDNKTKDPTHDVKTPNTETRTSCLIKYNVSTTPYLQSVKKKVQFDGTNSAFKELKFLTPVRRSRRLQEKTSKLPDMLKDHYPCVSSLEQLTELGRETDAFVCRPNAAC"
  },
  {
    "reference": "MKVVLLKDVAGLGIRGQVVKVAEGYGRNYLIPKGLAEEATPGRIKELARLDQAREERVERLAAQARKVAAGLKGLTVRIPARAGEGGKLFGSVGNKDIAAALAARHNLKIDRKKLELKEPIRSLGKFPVLARLHPGVQVEFEVEVVDNGT",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that can perform rRNA binding, structural constituent of ribosome in ribonucleoprotein complex, ribosome to support translation.2. The protein must exhibit the following characteristics: Binds to the 23S rRNA.. The designed protein sequence is ",
    "response#1": "MNKYVEE",
    "response#2": "MWNL",
    "response#3": "GFAAGQKVDVSGDTMGRGFAGLQKR"
  },
  {
    "reference": "MPKILDAIIKASKINKKLLAVLIDPEKFATENYSYFIEKLPEAVTHIFVGGSTATTAQSEVCVDFIKTKTNLPVILFPGDKEQITEKADGILLLSLISGRNPEYLIEQHIKAVPKLLNAGLEIIPTGYLLLDGGNQSAVARVSKTKPIQQDEIELIRNTALAGAMLGKQLVYLEAGSGALIPVSEKVIAEVKRDLNIPLIVGGGIRNATQLKKAYKAGADLVVIGTAFENGEFK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be able to compete with endogenous proteins or inhibitors for sn-glycerol 1-phosphate binding.2. The protein must exhibit phosphoglycerol geranylgeranyltransferase activity. The designed protein sequence is ",
    "response#1": "MQRSPL",
    "response#2": "EFIXDXIG",
    "response#3": "MKF"
  },
  {
    "reference": "MANTISAERRIRLTKKETAYNRYWRTTMKTYVKRAKKAIESGDREAAEAAVLKAQSVIDKVAVKGVIHKNEAARRKSRLMKLFNQKFQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Binds directly to 16S ribosomal RNA.2. The designed protein should have structural constituent of ribosome, rRNA binding within ribonucleoprotein complex, ribosome to regulate translation. The designed protein sequence is ",
    "response#1": "MGPIGAKVTLRGERMYDFLDKLVTVSLPRVRDFRGVSKKAFDGRGNYTLGVREQLIFPEIDYDQVSKVRGMDVVIVTTAKSDEESHELLTQLGMPY",
    "response#2": "MSPIGAKVTLRGERMYDFLDKLVTVSLPRVRDFRGVSKKAFDGRGNYTLGVREQLIFPEIDYDQVSKVRGMDVVIVTTAKSDEESHELLTQLGMPV",
    "response#3": "MASIPRGA"
  },
  {
    "reference": "MKLLKASLVALSLAASTFVYADNGLIAIITPSHDNPFFKAEADGAKQKAEELGYTTLVASHDDDANKQDQLISTAVSRKAKAIILDNAGSDVTVGALEKAKAAGVPAFLIDREINKTGVAVSQIVSNNYQGAQLSAEKFVELMGEKGQYVELLGRESDTNASVRSQGFHEIIDEYPEMKMVAQQTANWSQTEGFSRMESILQANPNIKGVISGNDTMALGAEAALKAAGRTDVIVVGFDGSDYVRDSILAGGNIKATALQPAWDQAQEAVVQADKYIRTGSTGKEEKQLMDCILIDSSNAKKLNKFSLSK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Part of an ABC transporter complex involved in D-apiose import (Probable).2. The protein should be designed to localize specifically to periplasmic space in order to perform its role in carbohydrate transport more effectively. The designed protein sequence is ",
    "response#1": "IAIITPAHDNPFFKAEAVGAEAKAKELGYETLVMTHDDDANKQSEMIDTAIGRGAKAIILDNAGADASVAAVKKAKDAGIPSFLIDREINATGVAVAQIVSNNYQGAQLGAQEFVKLMGEKGNYVELVGKESDTNAGIRSQGYHDVIDDYPEMKSVAKQSANWSQTEAYSKMETILQANPDIKGVISGNDTMAMGAIAALQAAGRKDVIVVGFDGSNDVRDSIKSGGIKATVLQPAYAQAQLAVEQADAYIKNAL",
    "response#2": "VTFDKATRVYPGSTKPAVDGLDIDIADGEFLVLVGPSGCGKSTSLRMLAGLEDVNGGAIRIGDRDVTHLPPKDRDIAMVFQNYALYPHMSVADNMGFALKIAGVNKAEIRQKVEEAAKILDLTEYLDRKPKALSGGQRQRVAMGRAIVREPQVFLMDEPLSNLDAKLRVSTRTQIASLQRRLGITTVYVTHDQVEAMTMGDRVAVLKDGLLQQVDSPRNMYDKPANLFVAGFF",
    "response#3": "IAIITPAHDNPFFKAEAVGAEAKAKELGYETLVMTHDDDANKQSEMIDTAIGRGAKAIILDNAGADASVAAVKKAKDAGIPSFLIDREINATGVAVAQIVSNNYQGAQLGAQEFVKLMGEKGNYVELVGKESDTNAGIRSQGYHDVIDDYPEMKSVAKQSANWSQTEAYSKMETILQANPDIKGVISGNDTMAMGAIAALQAAGRKDVIVVGFDGSNDVRDSIKSGGIKATVLQPAYAQAQLAVEQADAYIKNM"
  },
  {
    "reference": "MADSFLKKMEKLEAAEDFFKLLEIPYDIKVLRVYRLHILQRFHDYISAGSRDVGTQTEEQLKQYYGSLLEKAYRDFVHSTAQEEKVFKVFKEQQPASPRTEVRFVSLDSLIGGSKLKSKTL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be able to modulate nitrogen fixation in a way that leads to a desirable outcome.2. For general function, the protein need meet that May protect the nitrogenase Fe-Mo protein from oxidative damage. The designed protein sequence is ",
    "response#1": "CQPLGAILAAVGFEGTLPFVHGSQGCVAYFRTHFTRHFKEPFSGVSSSMTEDAAVFGGLKNMIEGLQNAYSLYQPKMIAVCTTCMAEVIGDDLGSFIGNAKADGSVPQDFPVPFAHTPSFVGSHITGYDNMMKAILLNLTDGKKPTTSNGKVNFIPGFETYVGNLRELKHLTSAMGVDATILGDNELYLDSPNDGEFKMYQGGTTLEEGADAINATKTIALQTYPTVKTLEYIEKEWQQPTATYRPWGIKGTDEFVMALSELTGNPVPPELELERGRAVDAMTDSHAWLHGKKAAIYGDPDLVMGMLQFMLEMGVEPVHVLVHNSTTEFEEEAKALLASSPYGQKATVWGGKDLWHLRSLLFTEPVDFLIGNSYGKYLWRDTKIPLIRIGYPIFDRHHLHRYSTIGYNGAINLLNWIVNGR",
    "response#2": "CQPLGAILAAVGFEGTLPFVHGSQGCVAYFRTHFTRHFKEPFSGVSSSMTEDAAVFGGLKNMIEGLQNAYSLYQPKMIAVCTTCMAEVIGDDLGSFIGNAKADGSVPQDFPVPFAHTPSFVGSHITGYDNMMKAILLNLTDGKKPTTSNGKVNFIPGFETYVGNLRELKHLTSAMGVDATILGDNELYLDSPNDGEFKMYQGGTTLEEGADAINATKTIALQTYPTVKTLEYIEKEWQQPTATYRPWGIKGTDEFVMALSELTGNPVPPELELERGRAVDAMTDSHAWLHGKKAAIYGDPDLVMGMLQFMLEMGVEPVHVLVHNSTTEFEEEAKALLASSPYGQKATVWGGKDLWHLRSLLFTEPVDFLIGNSYGKYLWRDTKIPLIRIGYPIFDRHHLHRYSTIGYNGAINLLNWIVNGH",
    "response#3": "CQPLGAILAAVGFEGTLPFVHGSQGCVAYFRTHFTRHFKEPFSGVSSSMTEDAAVFGGLKNMIEGLQNAYSLYQPKMIAVCTTCMAEVIGDDLGSFIGNAKADGSVPQDFPVPFAHTPSFVGSHITGYDNMMKAILLNLTDGKKPTTSNGKVNFIPGFETYVGNLRELKHLTSAMGVDATILGDNELYLDSPNDGEFKMYQGGTTLEEGADAINATKTIALQTYPTVKTLEYIEKEWQQPTATYRPWGIKGTDEFVMALSELTGNPVPPELELERGRAVDAMTDSHAWLHGKKAAIYGDPDLVMGMLQFMLEMGVEPVHVLVHNSTTEFEEEAKALLASSPYGQKATVWGGKDLWHLRSLLFTEPVDFLIGNSYGKYLWRDTKIPLIRIGYPIFDRHHLHRYSTIGYNGAINLLNWIVNGY"
  },
  {
    "reference": "MAALLLLSSAARVGVAAPLALRQQRPVVLPGGQLRTGSGAGAASAWAARPLRPELAAVSRPAVPARGRAPLFRPRAWMASSQIASSAFTWGTIAVLPFYTLMVVAPNADVTKRAVDSSAPYVALGILYAYLLYLSWTPDTLRAMFASKYWLPELTGIVRMFASEMTVASAWIHLLAVDLFAARQVYHDGIKNNIETRHSVSLCLLFCPIGIATHVLTKVLAGSIGRSH",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in chloroplast envelope that is critical for abscisic acid biosynthetic process, xanthophyll biosynthetic process.2. The protein must exhibit the following characteristics: In coleoptiles the MHZ4-dependent ABA pathway acts at or upstream of EIN2 to negatively regulate coleoptile ethylene response.; Required for neoxanthin biosynthesis, an intermediary step in abscisic acid (ABA) biosynthesis; Involved in an ABA pathway that acts at or downstream of ethylene receptors and positively regulates root ethylene response.3. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "YSRTWKGHVQNLLGYACSIYCVYKMLKSLQSVVFKEAGTKDPVTTMISIFLRLFDIGVDAALLSQYISLLFIGMLIVISVRGFLTNLMKFFFAVSRVGSGSSSNVVLFLSEIMGMYFLSSILLIRKSLRNEYRGIITDVLGGDIQFDFYHRWFDAIFVASAFLSLVLLSAF",
    "response#2": "VWRPKKSLSLSCRPSASVSAASSAVDVNESVTSEKPTKTLPFRIGHGFDLHRLEPGYPLIIGGIVIPHDRGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAASSVFIKEAVRLMDEAGYEIGNLDATLILQRPKISPHKETIRSNLSKLLGADPSVVNLKAKTHEKVDSLGENRSIAAHTVILLMKKS",
    "response#3": "YSRTWKGHVQNLLGYACSIYCVYKMLKSLQSVVFKEAGTKDPVTTMISIFLRLFDIGVDAALLSQYISLLFIGMLIVISVRGFLTNLMKFFFAVSRVGSGSSSNVVLFLSEIMGMYFLSSILLIRKSLRNEYRGIITDVLGGDIQFDFYHRWFDAIFVASAFLSLVLLSAFV"
  },
  {
    "reference": "MMLSLNNLQNIIYNPVIPFVGTIPDQLDPGTLIVIRGHVPSDADRFQVDLQNGSSMKPRADVAFHFNPRFKRAGCIVCNTLINEKWGREEITYDTPFKREKSFEIVIMVLKDKFQVAVNGKHTLLYGHRIGPEKIDTLGIYGKVNIHSIGFSFSSDLQSTQASSLELTEISRENVPKSGTPQLRLPFAARLNTPMGPGRTVVVKGEVNANAKSFNVDLLAGKSKDIALHLNPRLNIKAFVRNSFLQESWGEEERNITSFPFSPGMYFEMIIYCDVREFKVAVNGVHSLEYKHRFKELSSIDTLEINGDIHLLEVRSW",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a high specificity for a carbohydrate binding, with minimal off-target effects.2. The target protein must exhibit strand-strand-strand-strand-strand-turn-strand-strand-strand-strand-strand-strand-strand-helix-strand as its primary conformation. The designed protein sequence is ",
    "response#1": "HWANLQKRTPLMQVPVLVDLNGNHLWVNCEQQYSSKTYQAPFCHSTQCSRANTHQCLSCPAASRPGCHKNTCGLMSTNPITQQTGLGELGEDVLAIHATQGSTQQLGPLVTVPQFLFSCAPSFLVQKGLPRNTQGVAGLGHAPISLPNQLASHFGLQRQFTTCLSRYPTSKGAIIFGDAPNNMRQFQNQDIFHDLAFTPLTITLQGEYNVRVNSIRINQHSVFPLNKISSTIVGSTSGGTMISTSTPHMVLQQSVYQAFTQVFAQQLPKQAQVKSVAPFGLCFNSNKINAYPSVDLVMDKPNGPVWRISGEDLMVQAQPGVTCLGVMNGGMQPRAEITLGARQLEENLVVFDLARSRVGFA",
    "response#2": "HWANLQKRTPLMQVPVLVDLNGNHLWVNCEQQYSSKTYQAPFCHSTQCSRANTHQCLSCPAASRPGCHKNTCGLMSTNPITQQTGLGELGEDVLAIHATQGSTQQLGPLVTVPQFLFSCAPSFLVQKGLPRNTQGVAGLGHAPISLPNQLASHFGLQRQFTTCLSRYPTSKGAIIFGDAPNNMRQFQNQDIFHDLAFTPLTITLQGEYNVRVNSIRINQHSVFPLNKISSTIVGSTSGGTMISTSTPHMVLQQSVYQAFTQVFAQQLPKQAQVKSVAPFGLCFNSNKINAYPSVDLVMDKPNGPVWRISGEDLMVQAQPGVTCLGVMNGGMQPRAEITLGARQLEENLVVFDLARSRVGFS",
    "response#3": "HWANLQKRTPLMQVPVLVDLNGNHLWVNCEQQYSSKTYQAPFCHSTQCSRANTHQCLSCPAASRPGCHKNTCGLMSTNPITQQTGLGELGEDVLAIHATQGSTQQLGPLVTVPQFLFSCAPSFLVQKGLPRNTQGVAGLGHAPISLPNQLASHFGLQRQFTTCLSRYPTSKGAIIFGDAPNNMRQFQNQDIFHDLAFTPLTITLQGEYNVRVNSIRINQHSVFPLNKISSTIVGSTSGGTMISTSTPHMVLQQSVYQAFTQVFAQQLPKQAQVKSVAPFGLCFNSNKINAYPSVDLVMDKPNGPVWRISGEDLMVQAQPGVTCLGVMNGGMQPRAEITLGARQLEENLVVFDLARSRVGFA"
  },
  {
    "reference": "MKNFLKNLKLKRRIIAVLAAMHFFGNASVKLKIVGVTGTNGKTTTSTLLYKIATQLGHKAGLIGTVENIIVDERRPALITTPSPINLNKLLNEMASKGCEYVFMEVSSHALDQKRLAGLKFAGGIFTNLTHDHLDYHKNFANYFRAKKKFFKMLPKSAFALTNADDESGQKMLEGIKARKFSYGFKENTEVKPMYFNGEIKRLDFNGLELKFNQTQVKSKLLGKFNAYNLLAIWSASKLLGFDMNKVNKILEDIDPPRGRFEHFTSKNGVLVILDYAHTPDALENVFMTIREIKPKTGKIISVFGCGGDRDSSKRPKMGSIGASFSDIAIFTSDNPRSEDPDKIIYDMMDALYPDTRAKVKLISDRREAIREAVKLAQKDDVILCAGKGHENYQEIQGVKHHFDDMEELKKAYAKL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Ensure the presence of a signal peptide within the protein.2. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.3. The desired protein should be able to perform ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate4. The protein design should consider the endogenous proteins and enzymes present in the peptidoglycan biosynthesis, Cell wall biogenesis pathway to avoid interference with native metabolic processes.5. Incorporating a Meso-diaminopimelate recognition motif motif is critical for the successful design of the protein.6. The designed protein should have a binding site that is specific to the target ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate ligand.7. The protein contains novel Mur ligase central, Mur ligase C-terminal domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MSETGTNGKTTTSYLVEGGLRGAGRTPGVIGTVEMRVGEERIKSERTTPEATDLHAILGVMAERGADAVTMEVSSHALVFGRTDGVAYDVALFNNLTPEHLDFHPDMEDYFQAKARLFQPGKSRHGVVNLDDDYGRRLAAEAKIPVTTFSAAGAPEADWRAVDVQLGPVGSTFRVLGPDGAEADAAVPLPGPFNVANALAAITAMLALGFERERVLQALETFAGVPGRLEKIEAGGARPFRAYVDYAHTSDALERALNALRPVSKGRVITVFGCGGNRDRTKRPKMGGISARLSDVTIITSDNPRDEDPIEIIKEIEAGIEGLAKKRPDEPLSGHCYAVIPDRTEAIWKAVSEVASGDTILVAGKGHETYQIIGKTKHHFDDREEAKAALAARKTKGGA",
    "response#2": "MSVTGTNGKTSTTFFAHQIMFKLGFPSACLGTTGLWIMGPEGTIPVTERSKLTSPDAVTLHKILEKLYLRGIESVALEASSHGLHQNRLKGLNFKAAAFTNFSQDHLDYHRTMEDYFEAKCLLFEEILETEGWAVLNADIQEIGILTKIAERRCHRLFTYGIKGADVRLLKYEPKLDRLEITVNLWGIVKTLTLPLIGDYQVHNALCALGLAIGCGLDEAAVLRAIESFGGVKGRLERAAINRAGAPVYVDYAHTPDALEAAIFALRPHVTGRLITVFGAGGDRDTGKRAPMGEIVAAHSEVAIVTDDNPRGEDPEAIRAAIVAGAGAADNIAEIAGRREAIATAIAQAGKGDIVLIAGKGHETYQIIGREKHPFDDREQASVALAAREGMA",
    "response#3": "MKLKDILYKVAIEAVHGSTEIAVTGLENDSRRIQKGDLFVAYPGAATDGRLFIDKAVSAGAAAVVYDPIYFPENVVLPQSVPCVPVSDLALHLGSIAKYFYGNPSKELKLVGVGVTGTNGKTTVTYLLESILAAAGHKVGLIGTIEAIIGKEEIPASNTTPESYVIQKYFAEMVEAGCDSVVMEVSSQGLMLHRTQGFVFDYGIFTNLEPDHIGPGEHKDFEDYLACKGLLFKQCKVGIVNKDDKHWQEVIKGKTCMLETYGLSQDADLHASNVKSTPQGLVFTIDTLLGKGEFNVDVPGLFSVYNALTAIAICRHFEVPQENIKAALEEAVVKGRFQTITGKGIVAIVDYAHTPDALQNVLNTIATINEGKGKIITVVGCGGNRDKGKRPQMAAIAAENSDTTILTSDNPRDEDPDTIIAEMANGLSPNLKSKTLEIMDRRQAIKSAVMLAKEGDIVLVAGKGHEDYQEIKGVKHHMDEREIVAEILGER"
  },
  {
    "reference": "MKFGFDIDDTLIDLRQHAFHLYNKKLNKKVGLDVFHSLKTIEIHEAFGMTSEEGSHMWNSLLDEIYYTSCSPFPYAVETLQELEKQGHEIYYITARPKEHGEQTKKWLIEKGFPVHEDRFFYGMKDEEKIHFIQEIKLNYFFDDKPAVLETLIGKPINVYAKTTSYNQHLNIPRITSWTELGDIIKKEM",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have 5'-nucleotidase activity to facilitate deoxyribonucleotide catabolic process. The designed protein sequence is ",
    "response#1": "ILISNDDGVLAEGVRCLAAAAASRGHRVTVVCPDHERSATGHGLTIHTPIRAERVDELYGPGVKAWSCSGTPADCVKLALSELLAEKPDLVLSGVNHGPNLGTDVFCSGTVAAAMEGTLEGLPALAVSVACFQWRDFQAAAQLAMDVAENALADHWPNNLLLNLNIPPCHLEQMGSLRWTRLSIRHYR",
    "response#2": "ILVGNDDGIFAPGVRALANTLAPDHEVTVVCPDRERSATGHGLTIHQPIRAEQVQSMFVDQVTAWACSGTPADCIKLALGALLDSPPDFVLSGINQGPNLGTDVLYSGTVSAAMEGVIEGITSIAFSYSSFTDQQFQPAANFGQQLLEHLIQHPLSEPMLLNVNVPAVPADQIQGVALTRQGIRRYR",
    "response#3": "ILVGNDDGIFAPGVRALANTLAPDHEVTVVCPDRERSATGHGLTIHQPIRAEQVQSMFVDQVTAWACSGTPADCIKLALGALLDSPPDFVLSGINQGPNLGTDVLYSGTVSAAMEGVIEGITSIAFSYSSFTDQQFQPAANFGQQLLEHLIQHPLSEPMLLNVNVPAVPADQIQGVALTRQGIRRYR"
  },
  {
    "reference": "MPLNLPDKLPAIELLKEENIFVIDNSRASAQDIRPLKIVILNLMPLKITTETDLVRLLSNTPLQLEISFMKLKSHTSKNTPVEHMKAFYHDFDLMRDEKYDGMIITGAPVEQMPYEDVNYWDEITTIFDWARTHVTSTLYICWAAQAGLYHFYGVPKYPLKQKMFGIFRHHINVQGLPIFRGFDDEFFVPHSRHTEIHREDILKVKELALIAESDESGVYMAMARNGREFFITGHSEYSPYTLDTEYKRDLAKGLPIEMPVNYYKDDNPDNAPVVRWRGHANLLFSNWLNYYVYQETPFDINQIR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The desired protein should be able to perform acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine The designed protein sequence is ",
    "response#1": "LTPGVF",
    "response#2": "GVVD",
    "response#3": "LAVLFSNYFFN"
  },
  {
    "reference": "MTTMNISTLRLDSNPITTSTKSTTHRSGALGYNGSYSCRLLQFQKKNKAPSIIVCSTKPLASVVEHQGVNDSGLTRIESLSQVSGVLGCQWGDEGKGKLVDILAKHFDIVARCQGGANAGHTIYNSEGKKFALHLVPSGILNEETICVIGNGVVVHLPGLFKEIDNLESNGVSCQGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTKRGIGPCYSSKVIRNGIRVSDLRHMDTFPQQLDLLLSDAASRFQGFNYGRDMLKEEVERYKKFAERLEPFITDTVHFMNDAISQKKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRVVGDLVGVVKAYTTRVGSGPFPTEIMGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALRFCCQINGFASLNLTKLDVLSDLSEIQLGVTYRHPDGSTLNSFPSDIRLLEQIKVEYEVMPGWQSDISSVRKYSDLPKAAREYVERIEELVGVPVHYIGVGPGRDALIYK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should interact with enzymes and substrates within the AMP from IMP: step 1/2 pathway to enhance or regulate its activity.2. A protein with GTP binding functions is required to enable IMP metabolic process.3. The protein must exhibit the following characteristics: Catalyzes the first committed step in the biosynthesis of AMP from IMP ..4. The designed protein should have a high specificity for IMP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MPIGVPKVPFRSPGEED",
    "response#2": "MPITLNL",
    "response#3": "MPTIL"
  },
  {
    "reference": "MGGKWSKSSIVGWPEIRERMRRAQPAAEGVGAASQDLDTYGALTSSNLGPANADCAWLEAQEEGGDVGFPVRPQVPLRPMTYKGAFDLGFFLKEKGGLDGLIYSKKRAEILDLWVYHTQGFFPDWQNYTPGPGVRYPLTFGWCYKLVPVDPEEVEEDNKGDNHCLLHPMSQHGIEDEHREVLRWKFDSSLARRHLAREKHPEFYKDC",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. Incorporating a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif is critical for the successful design of the protein.2. The protein must exhibit the following characteristics: Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis.3. A protein that contributes to suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I by functioning in membrane, host cell plasma membrane, extracellular region, host cell Golgi membrane, virion component with GTP binding. The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPEIRERMRRTPPAAEGVGAVSQDLDKHGAITSSNINNADSVWLRAQEEEEVGFPVRPQVPLRPMTFKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYNTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVDPKQVEEANEGEDNCLLHPMNQHGMDDEEKETLMWKFDSRLALKHRAQELHPEFYKNC",
    "response#2": "MGGKWSKRSMPGWPAIRERMRRTEPAADGVGAASRDLERHGAITSSNTAATNADCAWLEAQEDEEVGFPVRPQVPLRPMTFKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGTRYPLTFGWCFKLVPVDPDQVEKANEGENNCLLHPMSQHGIEDADREVLMWKFDSRLAFHHMAREKHPEYYKNC",
    "response#3": "MGGKWSKSSIVGWPAIRERMRRARPAAEGVGAVSRDLDKHGAITSSNTAQNNADCAWLEAQEDEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVEPEQVEEANEGENNSLLHPMSLHGMEDAEREVLMWKFDSRLAFHHMARELHPQYYKDC"
  },
  {
    "reference": "MITSIILAAGEGTRMKSSLPKVLHKVCGKSMVNCVVDTCKKANINKNVVVVGHGKENVIKSLEAENVNFAVQPIGEDIPYGTGYAVMQAKDYIKDNEYVVILYGDTPLITSKTLTNLIDYHKNGKYAATVLTADFQDPTGYGRIIRDNKGNVVEIVEHKDATDEQREIKEINSGIYCFYGKYLKEALDNLTNDNAQNEYYITDAIKILKDKGLEVGGYKLEDSTDIKGVNTRVQLAEAEKIMRKRINEKLMLSGVTIIDPDNTYIGSEVEIGKDTVVYPGAILEGKTKVGSECIIGHNSRIADSVIGDRVNIQISTIIESKVDDDTKIGPYAYLRPNSDIGKSVKIGDFVEVKNSKINDNSKASHLAYIGDAEVGKDVNIGCGVVFVNYDGKNKHKTIVEDKAFIGCNVNLVSPVTVRKGAYIAAGSTITDEVPEESLAIARSRQTNKEGWVKKKNSSNK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.2. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.3. The presence of Nucleotidyl transferase domains is critical for the interaction of the designed protein with its intended targets.4. The designed protein should be involved in cell morphogenesis, peptidoglycan biosynthetic process, lipid A biosynthetic process, UDP-N-acetylglucosamine biosynthetic process, regulation of cell shape by localizing to cytoplasm and exhibiting UDP-N-acetylglucosamine diphosphorylase activity, magnesium ion binding.5. The designed protein should have a binding site that is specific to the target UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA, Mg(2+) ligand.6. The protein should be optimized for activity in Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "VILAAGFGTRMKSDLPKVLHPIAGRPMVEWAVRTAEQVGDLPPVVVVGHGKERVADLLKERVQYAEQKQLLGTGHAVMHAEAALRDKSDAVLVFYADMPLLQASTLRNLVELFAAEPRPGLLLARLTDPTGYGRIVRTAAGAVRGIVEHKDASEAERAIDEINTGIVAAPAGLLRRWLGALTADNAQGEYYLTDIVAQAVADGVPVAAAQPADVAEVEGANDRLQLAALERRYQRRLAERMMTAGVSLIDPESTYIDVNVTIGQDTVIEPGVYLKGETEIGQDAHIGAHSIIEDSQLGDGVVVKQSTIEEAIIGPNSDIGPNSHLRPGAILQESVHVGNYVEIKKSLVKSKAKLGHHAYVGDAEVGQGVNVSCGVIFANYDGKDKHRSVIGDYAFIGSNVTIVSPVTVEAGAFLAAGSTITEDVKQDSLAIARSRQRNIEGWTRPVRKKKK",
    "response#2": "VILAAGMGSRMNSDLPKVLHKIAGAPLLVHAITSGAAVNPSRLVVVAGHGADQVRSAVEDWAPEAEVVIQAEQLGTGHAVLQAREALEGFEGDVIVLYGDTPFIQPDTIARMVEARQQHDVVVLGFEAANPTGYGRLIVEDGALVAIREHKDASEEQRAITEVNTGTYAFDAAVLRDALGRLSTDNAQGELYLTDVLGIARGDGHRVGAHLVADARLTEGVNDRVQLATLGAELNRRTLRAHMQA",
    "response#3": "GIILAAGKGMRMKSNVAKPLHKVCGMTLVGHTVRIMRMAGIDEINVVVSTDLAESSELNEALPTDVQVAIQTEQLGTADAMISAKSSVLGHDSIVVAPADMVLVTADTVREMVELHSNSGAFATVLTMTLDDPTGYGRILRNQVGQVQRIVEEKDATDQERAINEVNTGIMVMPTARLKRWLGALSNKNAQGEYYLTDIIAMAVADGLVVQTEQPNTIWETLGVNSKVQLAELERIHQRNVANALLLQ"
  },
  {
    "reference": "MKYIFITGGVISSLGKGLTAASLGTLLEARGLRVVLQKFDPYLNVDPGTMNPFQHGEVYVLDDGAETDLDLGHYERFTNCVLTRHNNLTSGQVYESVILKERRGDYLGKTVQVIPHVTDVIKGRIREVSDESKYDVVITEIGGTTGDIEGLPFLEAIRQFILEVGTQNVLNMHVTLVPYIKAAHELKTKPTQQSVAKLREIGLQPQVLICRTERMLDLDLRQKLSMFCSVAEKAVIEERDVAHTIYEVPLTLHAEGLDQLVCDLLGLKTRQPDLAEWKDFVKRVISPAKRVRIAVIGKYFEVRDAYKSIYESLTHAAASEDCGIELELIEAQSLQDGVNGQLKDVSGVLIPGGFGLRGVEGKIRATRFAREHKIPYLGLCLGMQVATIEFARNACGIKEANSTEFDKNAKEPVISLLEEQRGVKNMGASMRLGTWPTKIANGTLAEKIYRSSEVTERHRHRYEFNMKYRDRMNDKGFVISGTSPDGTLAELIELRDHPYFVGCQYHPEFQSKPNKPHPLFKGFIQACLEYQSRATRAPSDRESPHQVPERELVLQQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be able to perform metal ion binding, CTP synthase activity, glutaminase activity under specific conditions.2. The protein design should take into account the physiological conditions of the CTP from UDP: step 2/2, CTP biosynthesis via de novo pathway pathway.3. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.4. The designed protein should have a binding site that is specific to the target ATP, Mg(2+), L-glutamine ligand.5. The Nucleophile active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "LKDAYFSLIEALDHAGIHNDTKVLISFVNSEELDAKNYKKNLKDSDAILVPGGFGDRGIEGMVLAARYARETKIPYFGICLGLQIAIIEMARNLLKLKDANSTEFNKKTKNNVISLVTEWKNEDGSVEKRDQKSDKGGTMRLGAQKASLKKGTLAKKLYKNSSIFERHRHRYEVNPEFIPSLEEKGLIISARSEMHNLVEMIELKEHPWFVACQFHPEFTSKPKNGHPLFNDFVLKGAEYTANLHDAYVSIREAFIHAGAALDTKVEIEWIESEGFENDPESVSVLKDYDGILVPGGFGSRGIEGKINAIKYVRENKIPYFGICLGMQMMVVEYARNVCGLKGANSTEFDENTKSPVIDLLPEQRAIKDKGATMRLGAWKCELAKGTRAFEAYGKQSIEERHRHRYEVNNDYLETLEKSGIVLSGRSGHIVEMVEWKEPHGFGIGLQAHPEYKSRLEEPAPLFVAFVKT",
    "response#2": "LKDAYFSLIEALDHAGIHNDTKVLISFVNSEELDAKNYKKNLKDSDAILVPGGFGDRGIEGMVLAARYARETKIPYFGICLGLQIAIIEMARNLLKLKDANSTEFNKKTKNNVISLVTEWKNEDGSVEKRDQKSDKGGTMRLGAQKASLKKGTLAKKLYKNSSIFERHRHRYEVNPEFIPSLEEKGLIISARSEMHNLVEMIELKEHPWFVACQFHPEFTSKPKNGHPLFNDFVIDGLERFERDADKSLCEALTHGGIANDTRVNIEWIEAESFEKEPHTVAERLKDKKAILVPGGYGERGTAGMISAIQYAREHQIPYMGICFGMQLAVIEVARHLAGIEKANTTEFGVTAEPVVGLLTEWLNGDVKERRSAEDDLGGTMRLGLYPAILAPGSLIQKIYGTSSIQERHRHRYELNIEYREKFEKAGLKFSGLSPDGLLPETVELEGHPWFVGVQFHPELRSRPFDPHPLFTSFIE",
    "response#3": "LRDAYMSVVESLRHAGLHQELDIDIRWVAAEQIEREGPERLLANVFGIVVPGGFGDRGIEGKIMAADYARVNNIPYLGLCLGMQCATIAFARQVLGTNAVNSTEFDPQVEHPVIDLMPDQRDVTNKGGTMRLGIYPCVLEPGTRAAEAYGETEVQERHRHRFEFNNQYRSQLEAAGLVISGHSPDNRLVEIIELRDHPWFVASQFHPEFLSRPNKPHPLFREFVRDCAA"
  },
  {
    "reference": "MAAVPQNNLQEQLERHSARKLNNKLSLSKPKSSGFTFKKKVPSANDVSVTSVSVAKTPVLSDKDVNITEAFSFSEPLPHTTNQQTGINDFKSAPSGPQTKRVGSKPLLPSLSQVPQEVSCTAQNTPVIKQSLNATFKKLEFSSSSDSLIAFNDWDDMDDFDTSGNSKAFVTPCKNHFVRVSTAQKSKKSKRNLVKAQLNKTHTGETDLAPFSSESKQEHLTEKQKDDSEWLSSDVICIDDDPDPEELINEDTQENHSLKTHLGAERGNCEKKKNLEETELRSVEKSSCIELDEDDYDIDFVPPSPEEGMTSTSSSSLKCSSMLKDLDTSDKKHSLSTSEDLSKLKEMATQQPNQETSTSCEARQTSLQQQLIRVMDRICKLVDAVPDDELKALDCGNELLQQRDIRRKLLAEADFDPSDASVLSAMWGCRPDPLESPVKDLASVSLPYAFRKGDSCLAGNAVKEFTLPHLPSNSMSTRERLLTPPLGKTGVLATTKSPFERPLFSSHLQKSFVSSNWAETPRAEKRNESPDFPGNVLTSTAVKDQNKYTTSINDLERELQAACEIDNFDIDDFDDDDDDWENLMQNLAASKSSTAAYPPIKEGRPVKSVSERPSSAKTDCLPGASTTQNKNFSESIPNYPDKMEQNLASRNPKHERFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATSIYLQLSK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein have HRDC, Helicase ATP-binding domains with specific sequence motifs that are crucial for its activity.2. A protein that contributes to regulation of cyclin-dependent protein serine/threonine kinase activity, DNA double-strand break processing, mitotic G2 DNA damage checkpoint signaling by functioning in cytosol, nucleus, cytoplasm with 3'-5' DNA helicase activity, zinc ion binding, 8-hydroxy-2'-deoxyguanosine DNA binding, protein homodimerization activity, DNA/DNA annealing activity.3. The protein should be designed to catalyze the following reaction ATP + H2O = ADP + H(+) + phosphate.4. The designed protein's functionality will benefit from Polar residues, Basic and acidic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "MAAVPQNNLQEQLELHSARKLHNRLNLSKPKSSGFTFKKKASSGNSASVTSVSAARTPVLSDKDVNVTEGSSFSEPQACPAGPPARINDFFKNAPAGLQSRGAGSTPLLPDVSQTPQEASCTPRLAPAAKTPRDTVFKKLDFSSSLDSLGALNGWDDMDDFDTSGTPKAFAASPRKPVARVSTAQKSKRAKRNFCKAQLKKADAAEADLTPASAASKPVELAGAGKGDCDGVGGGVICIDDDDGPVSQVLILDSQESHSLETHLGGERDHPEDKKVLDTVELHSAEEVPCIELEDDDYDIDFVPPSPEEEVSSATSSSLKCSSMLKDLDTPHREKDVLSPAQDLLSKPEKLTPQEPDPETTAGCEARPESHQLKAGSPRQQERHPNNKTQVLLLDTDEDDIDDSDSQQYDISPAEAKLVAKEIIRLHKEENVPFQDITLLVSSRTRNDGILQTFDRYGIPLVTDGGEQNYLKSVEVMVMLDTLRSIDNPLNDYALVALLRSPMFGFNEDDLTRIAIQDVKMAFYHKVKLSYHKEGHHSDLITPELSSKIDHFMKTFQTWRDFAKWHSLYDLIWKIYNDRFYYDYVGALPKAEQRQANLYALALRANQFEKTGFKGLSRFIRMIDKVLEESHQLKAGSPRQQERHPNNKTQVLLLDTDEDDIDDSDSQQYDISPAEAKLVAKEIIRLHKEENVPFQDITLLVSSRTRNDGILQTFDRYGIPLVTDGGEQNYLKSVEVMVMLDTLRSIDNPLNDYALVALLRSPMFGFNEDDLTRIAIQDVKMAFYHKVKLSYHKEGHHSDLITPELSSKIDHFMKTFQTWRDFAKWHSLYDLIWKIYNDRFYYDYVGALPKAEQRQANLYALALRANQFEKTGFKGLSRFIRMIDKVLE",
    "response#2": "MGRSFSIVAPTGIGKTHFGLIMALYLARKGKKSYIMVPTSLLVKHLLDRASFFLEKLAARGEPKPTVIGYYSGMRKKEMEETLERIRNKDFSILITTDRFLYNRFELIKDVNFDFIFVDDVDSFLKSPKNIDKVVLLMGFSIDDIERALKEEEKKEPIKAVPVASVRFNPGRVLVVSGATLRGKRTKRIKIFRRLFGFEPGA",
    "response#3": "MGRSFSIVAPTGIGKTHFGLIMALYLARKGKKSYIMVPTSLLVKHLLDRASFFLEKLAARGEPKPTVIGYYSGMRKKEMEETLERIRNKDFSILITTDRFLYNRFELIKDVNFDFIFVDDVDSFLKSPKNIDKVVLLMGFSIDDIERALKEEEKKEPIKAVPVASVRFNPGRVLVVSGATLRGKRTKRIKIFRRLFGFEPGEEKLAKSLKANGIRAKHYHAYMEPVDRSAAHQKWVSGEIQVIVATVAFGMGIDKPDVRFVIHHSLPKSIENYYQESGRAGRDGLVYDAIKRSKGSLVFVNMRRKAEKTALELSKKIRNFLTKKELRELKELAESLEENPTNEKLAKALQGGVAFHHAGLGREERVLVEENFKKGLIKVVVATPTLSAGINTPAFRVIVRDTWRYSEFGMERIPILEIQQMMGRAGRPKYDEVGEAIIVSTTEEPSTVMERYIKGKPEKLFSQLSNESILRGQILALRPNQLEAVNATLQGKDVFVLMPTGGGKSLCYQLPAVVKSGKTHGTTIVISPLISLMQDQVEHLLNKNIKAFESHQLKAGSPRQQERHPNNKTQVLLLDTDEDDIDDSDSQQYDISPAEAKLVAKEIIRLHKEENVPFQDITLLVSSRTRNDGILQTFDRYGIPLVTDGGEQNYLKSVEVMVMLDTLRSIDNPLNDYALVALLRSPMFGFNEDDLTRIAIQDVKMAFYHKVKLSYHKEGHHSDLITPELSSKIDHFMKTFQTWRDFAKWHSLYDLIWKIYNDRFYYDYVGALPKAEQRQANLYALALRANQFEKTGFKGLSRFIRMIDKVLE"
  },
  {
    "reference": "MLMPLSFSLQTKEELARVKARHPCCRQAELVAFLRLGNLDGGQPGEETVLFTTPYPALARKVYSLAREFLACPVKVRNSRRQGKGRPVFRVVARARLKEIQDWLAGRAGVPEYPCCQAAYMRGAFLVTGSVNKPSGTHHLELIFPDAAMAGQMQGLMQQQELEPRLSRRQRGYVLYLKDSEQIIRALSLMGAYSAVLAYENVLIFKDMRNRVNRLVNCETANLTKTVETGLRQAENIRYLIATVGWDYLPPALREIAAVRLQHPEASLKELGEMLHPPVGKSGVNHRLRRLELIARQVRGQGREGYAPDDDLSRPRA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The H-T-H motif domain within the designed protein should exhibit high affinity and specificity towards its target DNA sequence.2. The protein must exhibit the following characteristics: Involved in cell division and chromosome segregation..3. The designed protein should have DNA binding to facilitate cell cycle, regulation of sporulation, cell division. The designed protein sequence is ",
    "response#1": "ECCMKSEILGVILIGNLFSNEKILRNTKVVTENAAFARRIYSIFRRIYGICPEVSIRRSSKLKKHVSYSLTLVPNQMINKILYDFGG",
    "response#2": "ECCMKSEILGVILIGNLFSNEKILRNTKVVTENAAFARRIYSIFRRIYGICPEVSIRRSSKLKKHVSYSLTLVPNQMINKILYDFGR",
    "response#3": "ECCMKSEILGVILIGNLFSNEKILRNTKVVTENAAFARRIYSIFRRIYGICPEVSIRRSSKLKKHVSYSLTLVPNQMINKILYDFGV"
  },
  {
    "reference": "MLPFDPSTRYDRQVRLWGEEGQASIGSTSACVLGSDSLATEILKSLVLAGVQSFYVVDDAKVEQADIGQNFFLHADDIGRSRAEATLEKLTELNPSVSGSASSQPPTALAMEDVEKLTTFSVVVAANQNEEIDTTFAKVLYNIRVPFICIKTFGLIGTIRICIKEHTIANSHEENPRPDLRLDAPFSKLIEMINETNLDEMTLEQLRHTPYILLHFKALEVFRKQRNDPEAFPSTTAERKELQAILMSFRRSSEESGTKDSENFDEAKAAVIRAFQRTTIGSSVKSILSSPQCSTSTRPFWLICEALRRFVTENNNLLPLRGTLPDMTSDSSRYTRLATLFHEKALSDAQEVLRLTREVEKERGVGDVISDDVCYRFCKNADRIRVQYGDVLDYNEETKAIVEKIRESNIDEETRNQKVDEATWMLLMRAVGRFQKEKGRYPGTNGVPVSIDAQDLKKRVEVLIREALKDEQDFTSISNKVTDTAIAEICRFGAAELHVISSYVGGIAAQEIIKLATNQYVPIDNTFIFDGHTQESATFKF",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Ideal protein should have a binding affinity for key enzymes in the Protein modification pathway to enhance the overall efficiency of the pathway.2. The protein must exhibit the following characteristics: E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-rfl-1 (uba-3) thioester and free AMP; E1 finally transfers NEDD8 to the catalytic cysteine of ubc-12; Regulatory subunit of the dimeric uba-3-ula-1 E1 enzyme.3. The protein design should aim to mimic or enhance the function of natural proteins involved in protein neddylation, protein modification by small protein conjugation, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "MRAAVDALELRLRTDAAYFRALYAYTFGLGLDAGGRRLSVETAIAYWSLFFLDHTYAVTVPAPRLRSWFEFLRAGDHSVSRDTWDMFPRFAQRFPDDTELLEHYNELASWPLVIDEYYEYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGLFTIVDGNVVAGEDVGNNFFLQKESIGKNRAQTSMELLQELNDDVTGNFVPESPEQLLDKDPSFFCKFTMVISTQLPESTLLRLAETLWDSNIPLLVCRAYGFVGYMRIIVKEHTVIESHPDNALEDLRLDQPFPELREHLQSYDLDHMERKDHSHTPWIIVVAKYLDKWRSENGGQMPKSYKEKESFRDLIRQGILKNENGVPEDEENFEEAIKNVNTALNITKVSSSVEEILNDDRCTNLTHQSTSFWILARAVKEFMAMEGKGNLPLRGTIPDMIADSDKFIKLQNIYREKAKKDASAVESCVSKLLQSVGRPPESISERDIRLFCRNCAFLRVVRCRSLEEEYGLDTAKKDDIVSLMENPDNEIVLYLMLRAVDRFQKQLGRYPGIYNYQIESDIGKLKSCLNGLLQEYGLSLTVKDEYIQEFCRYGAAEPHTIASFLGGAAAQEAIKIITKQFVIFNNTFIYNAMLQQVLIIGAGGLGCELLKDLALMGFGNLHVIDMDTIELSNLNRQFLFRRTDIGASKAECAARFINARVPTCRVTPHFKKIQDFDESFYQQFHLVVCGLDSIVARRWINGMLLSMLRYEEDGTIDTSSIVPMIDGGTEGFKGNARVILPGFTACIECTLDLFPPQVNYPLCTIANTPRLPEHCIEYVKIIQWEKQNPFGVPLDGDDPQHIGWIYERALERSNEFNITGVTYRLVQGVVKHIIPAVASTNAAIAAACALEVFKLATSCYDSMANYLNFNDLDGIYTYTYEAEKSENCLAC",
    "response#2": "MRAAVDALELRLRTDAAYFRALYAYTFGLGLDAGGRRLSVETAIAYWSLFFLDHTYAVTVPAPRLRSWFEFLRAGDHSVSRDTWDMFPRFAQRFPDDTELLEHYNELASWPLVIDEYYEYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGLFTIVDGNVVAGEDVGNNFFLQKESIGKNRAQTSMELLQELNDDVTGNFVPESPEQLLDKDPSFFCKFTMVISTQLPESTLLRLAETLWDSNIPLLVCRAYGFVGYMRIIVKEHTVIESHPDNALEDLRLDQPFPELREHLQSYDLDHMERKDHSHTPWIIVVAKYLDKWRSENGGQMPKSYKEKESFRDLIRQGILKNENGVPEDEENFEEAIKNVNTALNITKVSSSVEEILNDDRCTNLTHQSTSFWILARAVKEFMAMEGKGNLPLRGTIPDMIADSDKFIKLQNIYREKAKKDASAVESCVSKLLQSVGRPPESISERDIRLFCRNCAFLRVVRCRSLEEEYGLDTAKKDDIVSLMENPDNEIVLYLMLRAVDRFQKQLGRYPGIYNYQIESDIGKLKSCLNGLLQEYGLSLTVKDEYIQEFCRYGAAEPHTIASFLGGAAAQEAIKIITKQFVIFNNTFIYNAMLQYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGLFTIVDGNVVAGEDVGNNFFLQKESIGKNRAQTSMELLQELNDDVTGNFVPESPEQLLDKDPSFFCKFTMVISTQLPESTLLRLAETLWDSNIPLLVCRAYGFVGYMRIIVKEHTVIESHPDNALEDLRLDQPFPELREHLQSYDLDHMERKDHSHTPWIIVVAKYLDKWRSENGGQMPKSYKEKESFRDLIRQGILKNENGVPEDEENFEEAIKNVNTALNITKVSSSVEEILNDDRCTNLTHQSTSFWILARAVKEFMAMEGKGNLPLRGTIPDMIADSDKFIKLQNIYREKAKKDASAVESCVSKLLQSVGRPPESISERDIRLFCRNCAFLRVVRCRSLEEEYGLDTAKKDDIVSLMENPDNEIVLYLMLRAVDRFQKQLGRYPGIYNYQIESDIGKLKSCLNGLLQEYGLSLTVKDEYIQEFCRYGAAEPHTIASFLGGAAAQEAIKIITKQFVIFNNTFIYNAMLQ",
    "response#3": "MRAAVDALELRLRTDAAYFRALYAYTFGLGLDAGGRRLSVETAIAYWSLFFLDHTYAVTVPAPRLRSWFEFLRAGDHSVSRDTWDMFPRFAQRFPDDTELLEHYNELASWPLVIDEYYEYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGLFTIVDGNVVAGEDVGNNFFLQKESIGKNRAQTSMELLQELNDDVTGNFVPESPEQLLDKDPSFFCKFTMVISTQLPESTLLRLAETLWDSNIPLLVCRAYGFVGYMRIIVKEHTVIESHPDNALEDLRLDQPFPELREHLQSYDLDHMERKDHSHTPWIIVVAKYLDKWRSENGGQMPKSYKEKESFRDLIRQGILKNENGVPEDEENFEEAIKNVNTALNITKVSSSVEEILNDDRCTNLTHQSTSFWILARAVKEFMAMEGKGNLPLRGTIPDMIADSDKFIKLQNIYREKAKKDASAVESCVSKLLQSVGRPPESISERDIRLFCRNCAFLRVVRCRSLEEEYGLDTAKKDDIVSLMENPDNEIVLYLMLRAVDRFQKQLGRYPGIYNYQIESDIGKLKSCLNGLLQEYGLSLTVKDEYIQEFCRYGAAEPHTIASFLGGAAAQEAIKIITKQFVIFNNTFIYNAMLQYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGLFTIVDGNVVAGEDVGNNFFLQKESIGKNRAQTSMELLQELNDDVTGNFVPESPEQLLDKDPSFFCKFTMVISTQLPESTLLRLAETLWDSNIPLLVCRAYGFVGYMRIIVKEHTVIESHPDNALEDLRLDQPFPELREHLQSYDLDHMERKDHSHTPWIIVVAKYLDKWRSENGGQMPKSYKEKESFRDLIRQGILKNENGVPEDEENFEEAIKNVNTALNITKVSSSVEEILNDDRCTNLTHQSTSFWILARAVKEFMAMEGKGNLPLRGTIPDMIADSDKFIKLQNIYREKAKKDASAVESCVSKLLQSVGRPPESISERDIRLFCRNCAFLRVVRCRSLEEEYGLDTAKKDDIVSLMENPDNEIVLYLMLRAVDRFQKQLGRYPGIYNYQIESDIGKLKSCLNGLLQEYGLSLTVKDEYIQEFCRYGAAEPHTIASFLGGAAAQEAIKIITKQFVIFNNTFIYNAMLQ"
  },
  {
    "reference": "MLAQKQKLSPISVNQKIDYKDIDLLKLFITEQGKILPRRATGVTVQQQRQIAKAIKRARVLSLLPFVASNSI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have rRNA binding and be active in ribosome. The designed protein sequence is ",
    "response#1": "RFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLD",
    "response#2": "PKILITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSLQKGTFL",
    "response#3": "PKILITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSLQKGTFV"
  },
  {
    "reference": "MGGKWSKGRISGWEAIRERMRRTRPEQAAEGVGAVSRDLEQRGAITINNTASNNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIHSQKRQEILDLWVYHTQGYFPDWDNYTPGPGIRYPLTFGWCFKLVPVDPDLVEEATAGENNCLLHPMNQHGMDDPEREVLMWKFDSRLAFHHVAREKHPEFYKDC",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein localizes to the membrane. The designed protein sequence is ",
    "response#1": "SVHKLRPGDIDVIAAMGDSLTAGAGIFADNEFNPNLIGFALGDSLTTHKESQLNIAESGAESADMMYMAEMLVKKIKNDPRINVQKHWKLISLMIGANDFCNEICSVSSPWSILENHKVDLLQVLRILRDNLPRTFVALIPPPHLKSLVDIRKEKSSFKCFVTTDVECSCLFGLAFQRYISIYYDIMRQWQILDMEIADYPEFQRNDFVVVAQPTLMNISIPLDSDGYSDMTYLSSDCFHVSQKTNARFANGLWNNLLETVC",
    "response#2": "SVHKLRPGDIDVIAAMGDSLTAGAGIFADNEFNPNLIGFALGDSLTTHKESQLNIAESGAESADMMYMAEMLVKKIKNDPRINVQKHWKLISLMIGANDFCNEICSVSSPWSILENHKVDLLQVLRILRDNLPRTFVALIPPPHLKSLVDIRKEKSSFKCFVTTDVECSCLFGLAFQRYISIYYDIMRQWQILDMEIADYPEFQRNDFVVVAQPTLMNISIPLDSDGYSDMTYLSSDCFHVSQKTNARFANGLWNNLLETVC",
    "response#3": "SVHKLRPGDIDVIAAMGDSLTAGAGIFADNEFNPNLIGFALGDSLTTHKESQLNIAESGAESADMMYMAEMLVKKIKNDPRINVQKHWKLISLMIGANDFCNEICSVSSPWSILENHKVDLLQVLRILRDNLPRTFVALIPPPHLKSLVDIRKEKSSFKCFVTTDVECSCLFGLAFQRYISIYYDIMRQWQILDMEIADYPEFQRNDFVVVAQPTLMNISIPLDSDGYSDMTYLSSDCFHVSQKTNARFANGLWNNLLETVGKCAR"
  },
  {
    "reference": "MKVLKDILYKVSLLSTIGDMEVRVKDIVFDSRKVSKGSVFVAIAGTQVDGHEFIDKAIELGAKAVVCERLPESPSTSITWVQVKNTAQSLGILASNYFDNPSSEIKVVAVTGTNGKTTSVTLLHQLFMAMGYSTGLLSTVENKINEEIVPATHTTPDAVSIQALLRQMVEAGCTHCFMEASSHAIVQERIAGLKLIGAIFTNISHDHLDYHKTFDEYIKAKKKLFDDLPKDAFALVNGDDKRGMVMVQNCRGTHQTFALKSAADFKAKIISNTIEGLELDINGRQVWFRLIGAFNAYNLLGAFGTAVLLGEDEEEVLQHLSAIPGAKGRFDRITGAGITAIVDYAHTPDALENVLKTINGVRTGGEQVITVVGCGGNRDTTKRPIMAKIAVRESDKVILTSDNPRFEEPEQIIKDMQAGVGPTEMRKVLSIVDRREAIRTACMLCKKGDIILIAGKGHEDYQEIQGVKHHFDDAEVVGEILNQLSQN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein's functional design necessitates the inclusion of a Meso-diaminopimelate recognition motif motif.2. Ideal protein should have a binding affinity for key enzymes in the peptidoglycan biosynthesis, Cell wall biogenesis pathway to enhance the overall efficiency of the pathway.3. The UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP, meso-2,6-diaminoheptanedioate binding site should be stable and able to withstand changes in environmental conditions.4. The ideal protein would be one that plays a critical role in cell cycle, peptidoglycan biosynthetic process, cell division, regulation of cell shape, cell wall organization through ATP binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, magnesium ion binding within cytoplasm.5. The designed protein have Mur ligase central, Mur ligase N-terminal catalytic, Mur ligase C-terminal domains with specific sequence motifs that are crucial for its activity.6. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.. The designed protein sequence is ",
    "response#1": "MKLTDLLKGIPIISVDKDADTSIATDSRRVRPGALFFCLPGQHVDGHAFAPAAIAAGARAIVASREIDAPAGVGVAIVDDSQSALSRVAAEFYGNPSEKLKLVGVTGTNGKTTTTRFVTHGLRAVGMRVASLGTLGFDWNGALEPGIFTTPEADEVSRRLAMVRDGGGTHLVMEVSSHGLELHRVAGLTFDVAAFTNLTQDHLDFHGTMEAYARAKARLFDEFAPRAAVINVDDAFGRALVARVRGRCLTVGRSAQATVRALDARADARGIRAEVDAEGTRVALESPLIGAHNLENLLVALGVCASLGLPLKTISDALQNFAGVPGRMQQIENTNIFVDYSHTPDALEKALLELKNIQVLGSKLNVIFGCGGNRDKKKRSLMGQIAAKLADNVIITDDNPRLEDPKLIRAEIISGIDKANYIEIENREKAIKYGINNLNQDDILLIAGKGHEEYQILGTTKIHFDDREVAREALRGRC",
    "response#2": "MKLRDLANIIGARVTGDGPIAVTGLTVDSRAVKEGYLFAALPGAMMHGAEFIGPALRMGAAAILTDAEGAKIAAEFLAGADVALIIAEDPREALAGAAVVYDQPTARLMLVTGTSGKTSVAAFTRQIWLRLGHASASIGTIGLVSPKRTIYGSLTTPDPIALHRQLDEIARDGVTHLAFEASSHGLDQYRLDGVRIAAGGFTNLSRDHMDYHPDVAHYLAAKLRLFRDLVIADGAAVISADHDCSQAVIDAARARGLRIITVGRKGDGRGEGIRLVDARIDGFAQKLELEHRGRNISIRLPLVGEFQIENALVAAGLAIATGGDADAMFAALPRLQGVRGRFEQFHSADNIVGIVDYAHTPDALENVLETITHLRHGNEKVITVVGCGGNRDAEKRPKMAAIACKYSNLVILTSDNPRYEDPLDILEQMRKGVPAQDYKKTIVIPDRREAIFKAGLEANPEDIILIAGKGHEQGQIVGDRVLPFDDAEVVRELLGEVTA",
    "response#3": "MKLKELLKNISISEIKGNLDTEISSIIIDSRRVLPGSMFVALRGTQTDGHQFIETTVNAGASAVLCEALPEKLNDKVTYIQTEDSAAAMGMIAAAFYNHPSKKIKLIGVTGTNGKTSVATFLFQLFRALGYRCGLLSTVQNQIEDEIIPSTHTTPDAVALNKLLSEMLEKNCSHVFMEVSSHSVAQHRITGLHFAGGIFTNITHDHLDFHKTFDNYIKAKKGFFDQLPKSAFALVNVDDRRGSVMVQNTRAKVETYSLQTLASFKGKIISDTLAGMHMEINQQEVWFRVIGRFNAYNLLAVYGAICQKLGIEESVIQKGISKVVVPGRFETLRSDGGIFIVVDYAHTPDALENILDSINEIRTKNERLITVFGCGGDRDNAKRPEMGKIATRKSTLAIITSDNPRSENPEAIIKEIEAGVEPQNFSKYTSIPDRKEAIKMALKFAEPRDIILLAGKGHEDYQILGNTKIHFDDREELEKALNERM"
  },
  {
    "reference": "MNFNKLKFGDTIGIIGGGQLGKMMAQSAQKMGFKVICLDPNPDSPCKSVAHEFITAAYDDEEALHELGEKSDVITYEFENISAEQLIRLTQKFNIPQGYQAIQLLQDRLTEKQTLQKAGSKIVPFLPIKEEKDLNVVINQLGYPFIVKTRFGGYDGKGQVLVKNEESIQEAKDLISQQECVAEQFLDIALEVSLTVTIGNQKQITYFPLQENEHRNQILFKTIVPARSDKEQEARDEVNKIINEVHFVGTFTVEFFIDKSNNLYVNEIAPRPHNSGHYSIEACDYSQFDTHILAVTGQNLPKEIEILKPAVMMNLLGRDLDLLEDKFANHPEWHVHIYGKPERKPNRKMGHMTILTNNVDETEKAMLKQFEGRE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein should be able to manipulate the activity of enzymes in the IMP biosynthesis via de novo pathway pathway, either by activating or inhibiting their function.2. A protein with NAD binding, ATP binding, 5-(carboxyamino)imidazole ribonucleotide synthase activity, metal ion binding is needed to enhance 'de novo' IMP biosynthetic process in a specific context.3. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR)..4. The protein have enhanced 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ATP +   hydrogencarbonate = 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole +   ADP + 2 H(+) + phosphate.5. The protein should have a high affinity for ATP. The designed protein sequence is ",
    "response#1": "MKIGVIGGGQLGRMLALAGTPLGMNFAFLDPAPDACAASLGEHIRADYGDQEHLRQLADEVDLVTFEFESVPAETVAFLSQFVPVYPNAESLRIELLDELNLSTSPWCLLKDKNQWNDLFQTVGEKVVVKRRTGGYDGRGQWIIRDENRADITDDLFGEVIAEKFIPFDYEVSIVGARFKNGEKRFYPVTHNLQQNGILRYSVVDCAFPQQSVQQKQAETMLGKIMDKLGYVGVMAMECFVVGDKLLINELAPRVHNSGHWTQLGCSISQLYTGKAKSVYRTDDPEVYIMKFRDDITAFDGEKKDTLEGKGRYNAEVSTFIFRYLEEHGIRTHYLASIESGVIAVRNLTMIPLEVIVRNVAAGSIVRNYPFREGEPLDPPLIVIDYKSDAHHDPMLNDELIYALGLATPEELDQIKAMALAINELLSDYLDLRGITLVDFKMEFGRYNGEIVVGDEISMDSMRLWDKETGTSLDKDVYRFGKGDVMETYAGVAKVRKLYEGKAKIVYETDEKDKLILEFKDVATAFDGVKKEEIKGKGSLNNEISSLIFEILEDHGIKTHYIKKLSDREMLVWRAERFPVEVVVRNYAAGSFVKRYGVKEGTKLEQPLVEFFMKSDELHDPMVCINHIKVLKLAPAELVPEMEKIALKVNEILKKIFEEKGILLVDFKLEFGRLPSGELAIVDEISPDSMRLWDKSTGENWIKTDL",
    "response#2": "MFKDLGIPTPDFADVQSQADLDAAAAAIGLPAVLKTRTLGYDGKGQKVLRQPADVQGAFAELGSVPCILEGFVPFTGEVSLVAVRARDGETRFYPLVHNTHDSGILKLSVASSGHPLQALAEDYVGRVLARLDYVGVLAFEFFEVDGGLKANEIAPRVHNSGHWTIEGAECSQLYTGKAKSVYRTDDPEVYIMKFRDDITAFDGEKKDTLEGKGRYNAEVSTFIFRYLEEHGIRTHYLASIESGVIAVRNLTMIPLEVIVRNVAAGSIVRNYPFREGEPLDPPLIVIDYKSDAHHDPMLNDELIYALGLATPEELDQIKAMALAINELLSDYLDLRGITLVDFKMEFGRYNGEIVVGDEISMDSMRLWDKETGTSLDKDVYRFGKGDVMETYAGVAKLYSGKAKDVLLTDDPEIVAVRFRDDITAGDGEKKDTLEMKGYYNSVISAKIFEVLEEAGVPTQYLELREPGCILARKLEMIPIEVITRNIAAGSIVRRFPFTEGQEFVPPLIQMDYKSDEHGDPMLNDDIILALGIATRDELEEIRRITLHINSVLRDFLKSRGLILPDFKLEFGRDSSGRIRLGDEVSPDTCRLWDMETGEPLDKDIFRRGEEGVVGAYRRVARE",
    "response#3": "MKIGVIGGGQLGRMLALAGTPLGMNFAFLDPAPDACAASLGEHIRADYGDQEHLRQLADEVDLVTFEFESVPAETVAFLSQFVPVYPNAESLRITEAMKSGVKDFIGGNCTVSLMLMAMGIGWGAAEPLRRMLRILLERS"
  },
  {
    "reference": "MSTHPSRDVAQERDAADPLAGLRDRFVVDDLLYLDGNSLGRLPAATGDHLAGLVRDGWGTELVRSWPTWIEWPRRLGDRIAAHALGARDGEVIVSDSTSVNLYKLAAAALDARPGRRTILADAEEFPTDRYLLEGLAAQRGHTLRLLPSDVDEGLDQATLAAALTEDVALVVLSAVSYRCGALLDVAAVTEAARRVGALVLWDLSHAVGSVPLDLTAAGADLAVGCTYKYLNGGPGAPAFLYVRRELQEALRQPIWGWFGQRDQFRMGAGYDPAPGPERFLVGTPPVLGMAALDPALDVLADAGMDRVREKSVRLGELIIELADAWLVPHGFRLASPRDPDRRGGHVSLYHPEALQISRALVDAGVVGDYRVPDRLRLGPAALYTRHVDVWDAMDRLRDIAERRAWTEVPAEPSRVT",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively..2. A protein should be able to manipulate the activity of enzymes in the NAD(+) biosynthesis, Cofactor biosynthesis pathway, either by activating or inhibiting their function.3. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in tryptophan catabolic process, quinolinate biosynthetic process, L-kynurenine catabolic process, 'de novo' NAD biosynthetic process from tryptophan. The designed protein sequence is ",
    "response#1": "NQLAPLIGAGKDEVVCTDSTSINLYKVLSAALNIAREDSPARKRIVSERSNFPTDLYIAEGLCKERGLELVLVEPEEIAASLTSDVAVLMLTHVNYRTGAMHDMAAITAAAHAQGILCVWDLAHSAGAVPVDLNGAGADFSIGCGYKYLNGGPGAPAFVWVHPRHANRPDLKFMQPLSGWWGHAAPFAFTPDYQPAPGITRYLCGTQPIISLSALQCGLDVFTAAQGLGGL",
    "response#2": "SITDLVDPILARLVGASTSAEVACTGSLTTNLHILMTTFYRPTKERYKILCEANCFPSDLYAFRSQIEMHGLDPKYALISLLPRPGEFTLRPEDIISTINREGQSIALILFSGVQFYTGQFFPIKQITAAGHQQGCIVGWDLAHAIGNVLLELHDWQVDFAAWCSYKYLNAGPGAIAGLFVHEKWTSDPTKLGGQVLVGAKQNEVAVMGSLTSNLNALLIHFYKPKGKRTKILFEKQAFPSDYYAFLNIVQVFGYDASHLIQIEIPKGETYIKTETILDVFDKYEDEIAIVCLPGIQYYTGQFFDIAKITKHVKTSAPDVVVGWDLAHAVGNVPLSLHDWGVDFAAWCSYKYLNAGPGAIAGIFVNEKYTEQNKPENNK",
    "response#3": "LVGAKQNEVAVMGSLTSNLNALLIHFYKPKGKRTKILFEKQAFPSDYYAFLNIVQVFGYDASHLIQIEIPKGETYIKTETILDVFDKYEDEIAIVCLPGIQYYTGQFFDIAKITKHVKTSAPDVVVGWDLAHAVGNVPLSLHDWGVDFAAWCSYKYLNAGPGAIAGIFVNEKYTEQNKPENWK"
  },
  {
    "reference": "MRLFTSEEMKIAETHAIEKIGIPSIVLMENAGIKSLLTIEKIVSGIKGKRFTIICGKGNNGGDGLVIARHLHNNAVPVHLFLLSEPEELSPDSEINCRILIKSGLYPSVIKEKNDIDKLRVAMEFSECIIDCIYGTGIKDNIKGLASEIVKAVNLSRAIKISIDLPSGVCGTTGKASIPSIISNYTITLGTPKIGLFMYPGLAHAGEVWTADIGIPSISNSHPQGMHYLIDDELAFSLLPYRDENIHKGLAGKVLVFGGSSEYQGAGIMASGGALRSGAGIINLALPDCLMGKIICQIPPEVIVSYLPSRDGGFFLNESTAINLCSKYNAILVGPGWGRGEGRRQTLEHILKHYSGIVVIDADALNIISQSPELLANRKCRIIITPHHAEMARLCKTDIEDIANNQIARAKDYAADNKVTVVLKSSVTVIAAPNGKIFINSAPNSGMAKGGSGDLLSGFIAGLAATGGSELNAAITGVYIISEAGKEAKEQLGCDSMSVSEVSFLIPKVFSKLRNKK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a high specificity for (6S)-NADPHX binding, with minimal off-target effects.2. The protein design should be able to enhance the efficiency of nicotinamide nucleotide metabolic process.3. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.4. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration The designed protein sequence is ",
    "response#1": "SPMGQKFSRGHAAVTGGGVSSTGAARLAARAALRAGAGAVTLICPPSALMVYAVTLEAVMVASFAEVAEFENWLKERRIGTVLLGPGNGVSERTREFVIAALESSSNVVLDADALTVFQGDPETLFSLIGTKRDGEVVMTPHEAEFERIFSLSGSKLQRAREAAALSGAVVLLKGADTVIATPDGKAVINYNAPSDLATAGSGDVLAGLICGFLSGGMPAFSAAAAAAWVHGEAGAQ",
    "response#2": "DGHKYLRGHGVIAGGAEMTGAARLAARAARRAGAGLVTIAAPPAALGVYAAGDPGNIVIPVDGVAAFEKAMTDRRVGAVLVGPGAGREGDAAERTRALTRAALATAKPVVLDADALTVHAESPGDLFPTFDGPVVLTPHAGEFARLFADLEDVSKLTRARAAARRSGAVVVLKGADTVIAAPDGRAAINANAPPTLATAGSGDVLAGVLIGLLAQGMPPYAAARAAVWLHGRAAQHGGWGLIAEDIGDLLA",
    "response#3": "LAVTGSYGMCGASILTAKAALVSGAGLVKCFVCDKNYSAFTCAVPEAVVIPTETFPSGAMAVYGKQLSEGLDSTDALILGCGIGKSQEAANLVRVILETVEIPVVLDADGINALARNIEWLRKTKASVILTPHPAEMARLCGTSVAMIQANRPEFAKRVAVTYGKIVVLKGANTVVASPDGKIFFNTTGNPGMATGGSGDVLSGIIAARIALGDSPIEAAKNGVFLHGLAADNALKKYSMAGLLPSDIIAEG"
  },
  {
    "reference": "MQEIKEETKMKIIDFTWPAGFYSDGINAGLRQVKNDLGWLYSKVPAQAAGVYTTNQFQAAPTKLTKQTINAKHVLQAIVMNSANANSCTGAQGEADALAMQQLAATKLELKPALIGVASTGLIGKTLPMKLIKAGIKQLQLTKTTAVTNAILTTDKQPKTCSVQCKIAGKLVTISGFAKGSGMIHPKMATMLGFVTTDAAISGTQLQELLSQQVDATFNQITVDGDTSTNDMVVALANGLAGNQALVPNSPDYSKFKANFSYVLGELAKKIAADGEGASKLVEVNVHGAADKADGQKVAKAIVGSNLVKAAIFGEDPNWGRIMGAIGQTDAKLDISNVAIQMNGQALVKGSIAVDFDPTAMKLALAKPKIIIEVDLHNGVASGQAWGCDLTYNYVKINASYHN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in arginine biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.2. A protein that is capable of catalyzing reactions within the Amino-acid biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "YSMDRNMLHCSFEGGELEDPWNEPGPNSYVMAVPMEQAPDEAEYISIDFEHGNPVAVNGERLSPAALVKKLNSIGGRHGIGRLDMVENRFVGIKSRGVYETPGGTLIHIAHRDLEGICIDRETMHLRDAMLPRYAAAIYNGFWFAPEREAMQAMIDVSQQRVTGTVRLKLYKGNAWPVGRQSPNTLYCHDLATFEDCATYDHKDAAGFIKLQGLRIYSMDRNMLHCSFEGGELEDPWNEPGPNSYVMAVPMEQAPDEAEYISIDFEHGNPVAVNGERLSPAALVKKLNSIGGRHGIGRLDMVENRFVGIKSRGVYETPGGTLIHIAHRDLEGICIDRETMHLRDAMLPRYAAAIYNGFWFAPEREAMQAMIDVSQQRVTGTVRLKLYKGNAWPVGRQSPNTLYCHDLATFEDCATYDHKDAAGFIKLQGLRIL",
    "response#2": "YSMDRNMLHCSFEGGELEDPWNEPGPNSYVMAVPMEQAPDEAEYISIDFEHGNPVAVNGERLSPAALVKKLNSIGGRHGIGRLDMVENRFVGIKSRGVYETPGGTLIHIAHRDLEGICIDRETMHLRDAMLPRYAAAIYNGFWFAPEREAMQAMIDVSQQRVTGTVRLKLYKGNAWPVGRQSPNTLYCHDLATFEDCATYDHKDAAGFIKLQGLRIYSMDRNMLHCSFEGGELEDPWNEPGPNSYVMAVPMEQAPDEAEYISIDFEHGNPVAVNGERLSPAALVKKLNSIGGRHGIGRLDMVENRFVGIKSRGVYETPGGTLIHIAHRDLEGICIDRETMHLRDAMLPRYAAAIYNGFWFAPEREAMQAMIDVSQQRVTGTVRLKLYKGNAWPVGRQSPNTLYCHDLATFEDCATYDHKDAAGFIKLQGLRILA",
    "response#3": "YSMDRNMLHCSFEGGELEDPWNEPGPNSYVMAVPMEQAPDEAEYISIDFEHGNPVAVNGERLSPAALVKKLNSIGGRHGIGRLDMVENRFVGIKSRGVYETPGGTLIHIAHRDLEGICIDRETMHLRDAMLPRYAAAIYNGFWFAPEREAMQAMIDVSQQRVTGTVRLKLYKGNAWPVGRQSPNTLYCHDLATFEDCATYDHKDAAGFIKLQGLRIYSMDRNMLHCSFEGGELEDPWNEPGPNSYVMAVPMEQAPDEAEYISIDFEHGNPVAVNGERLSPAALVKKLNSIGGRHGIGRLDMVENRFVGIKSRGVYETPGGTLIHIAHRDLEGICIDRETMHLRDAMLPRYAAAIYNGFWFAPEREAMQAMIDVSQQRVTGTVRLKLYKGNAWPVGRQSPNTLYCHDLATFEDCATYDHKDAAGFIKLQGLRILAAVPA"
  },
  {
    "reference": "MAEPLAVETKPLSNGNANGNAVGIAESASAVFQEKLKLQQQEESNEIAAVPKYGLKLKFDHVWPEKRIQNVRASIRQTLPMVPLVCRYAAYYWKVSREGRRVYMDYYYMENGKQIYGVPIGGIGGGTIGRGYAGEFCRFQMRPGIYEYNVVLANQFIVTIKDPKGCTIFQSLLSKCSTRDKTSDPDGDPDGERTKCQLPNCSSRAKQPLSAWHSNIEDTRCSYTGLYPRSWTEYDLSHYGVRLTCRQVSPVIPHEYRESSLPCAVFVWSVENVCDQERKVSITFTFKNGTGNKKQDAEGGAESQLISEGNAKGVSIRQKISEMPCSYNLACRVLPEISITRCPQFDPAGNGEQLWAQLKEHGQLSEHPTSEALKTKDIGVAVCGQVALKPMASHDLEFVLAWDMPKIQFPRKMQTHTRYYTKYFDDSGDSGPRICEYALRQYSTWERLIDAWQRPILNDETLPDWYKCAIFNQLYFISDGGTIWLKCDSSLGKELAYDDPRLAYGRFGYLEGHEYRMYNTYDVHFYASPALAHLWPNLQVSLQYDFKDAIAAELNDTRKMLYDGKVMPRKVKNCVPHDLGDPDEEPFTLINCYNIHDVNDWKDLNTKFVLQVYRDYYVLNELAQAQSDNASKFSSIEFIDKESLYELYSQDNKRKNSADEKQQNRKSASMYINETNGKVYLMDAIGYLKAMYASCKAIMERTIEYDKDNDGLIENTKMPDQTYDSWVMDGPSAYCSGLWLAALQAMSAMATILDQPNDCLRYQDILEK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. We need a protein that can perform a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-   acylsphing-4-enine + D-glucose with high efficiency.2. The designed protein should contain Cytoplasmic, Extracellular topological domains.3. A protein that can perform glucosylceramidase activity in plasma membrane, membrane to support bile acid metabolic process, carbohydrate metabolic process, glucosylceramide catabolic process. The designed protein sequence is ",
    "response#1": "GRFGYLEGQEYRMYNTYDVHFYASFALIMLWPKLELSLQYDMALATLGEDLTWRRYLMSGVMAPVKRRNVIPHDIGDPDDEPWLRVNAYVIHDTADWKDLNLKFVLQVYRDYYLTGDQGFLRDMWPVCLAVMESEMKFDKDQDGLIENGGYADQTYDAWVTTGPSAYCGGLWLAAVAMMVQMAALCGTQDIRDKFSSILSRGQQAYERLLWNGRYYNYDCSSRPQSCSIMSDQCAGQWFLRGRFGYLEGQEYRMYNTYDVHFYASFALIMLWPKLELSLQYDMALATLGEDLTWRRYLMSGVMAPVKRRNVIPHDIGDPDDEPWLRVNAYVIHDTADWKDLNLKFVLQVYRDYYLTGDQGFLRDMWPVCLAVMESEMKFDKDQDGLIENGGYADQTYDAWVTTGPSAYCGGLWLAAVAMMVQMAALCGTQDIRDKFSSILSRGQQAYERLLWNGRYYNYDCSSRPQSCSIMSDQCAGQWFLRQ",
    "response#2": "AFERRHEFMALCMRDFVDHYNGWIWGHQGPQLLTRVFKKWCSIRSLAESRACRGVTTLPPEAFYPIPWQDWKKYFEDINPEELPRLLSATYAVHVWNKKSQGTRFEATSRALLAQLHARYCPTTHEL",
    "response#3": "TVLVTCGATVSFPRLVETVLDRSVTEKLKVLGYGRIVIQYGRGFSDTFLQLVEKHLGLFTEKKSCGIKVLDKIENLKVISVDGIEICGFEFSHDIEKLIANNIDLVISHAGTGSILDSLRVGKKLIVVVNDTLMDNHQQLIADKFEQQKLLWSVHANTEELLRALDRSENEELLKIL"
  },
  {
    "reference": "MPRIAYLGPQGTFTESALLQMISGAMVPGGDADDTAVTPVPTDSTPAGLEAVRSGAADYACVPIENSIEGSVLPTLDSLAVGAPLQIFAELTLAVSFSIVVRPDHDGDVGTVAAFPVAAAQVRRWLAEHLPAAQLVPAHSNAAAAADVAGGRADAGISTALAAERYGLRSLAAGVVDEPNARTRFVLVGRPAPPPARTGADRTSVALRLPNTPGALVAAMTELSIRDIDLTRIESRPTRTELGTYVFFLDCVGHLEDDAVAEALKALHRRCEDVRYLGSWPTGTAAGAPPPSSDEATRWLTRLREGLPTPPEGGR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must be able to protein homodimerization activity, chorismate mutase activity.2. The desired protein should be able to perform H(+) + prephenate = 3-phenylpyruvate + CO2 + H2O3. A protein that can modulate the overall flux of metabolites through the phenylpyruvate from prephenate: step 1/1, L-phenylalanine biosynthesis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "EPECHDNAMQLVQAMRHFSTFVYGLNLCKEEADIETLLKFSSPIYRLELAMVGRLFAQSPELYADIIFAQQESQHAIGDYLDNYREALELLKRGDREEFINQFFN",
    "response#2": "EPECHDNAMQLVQAMRHFSTFVYGLNLCKEEADIETLLKFSSPIYRLELAMVGRLFAQSPELYADIIFAQQESQHAIGDYLDNYREALELLKRGDREEFINQFLN",
    "response#3": "EPECHDNAMQLVQAMRHFSTFVYGLNLCKEEADIETLLKFSSPIYRLELAMVGRLFAQSPELYADIIFAQQESQHAIGDYLDNYREALELLKRGDREEFINQFL"
  },
  {
    "reference": "MSSAHRPFALSHGAIEHTILVPTTEFFKYAQLKEQFAKSLPEATEGYASDTEPASPAELFAKFVGFIADLVDPAVKGQFDDILPYVLHDFESRYFATSDIHSFAAHLLEDDAYPTTSAKVKDVIKHYFAAVLAAKVPIAKSSLVLLRASADRLARTFAIFGGQGNTDDYFDELRELNYMYSGLLTDFLGLVAAKLNALVKNTQDVDKIYTQGFDILAWLKAADKTPDQDYLLSVPVSCPLICVIQLCHYAVTCKTLGVTPGGLRDALVGATGHSQGLVTAVAIASSDTWDAFLANALKAVSLLFFIGARCLSAYPRTSLPPTMLQDSLDHGEGRPSPMLSIRDLSRAEVERFIESTNEHLPQSKHLAISLVNGGRNLVVSGPPESLYGLNLTLRNNKAPSGLDQARVPFSERKLKFSNRFLPIFAPFHSHLLQPANAMILADMEREQLHFSAADLMIPVFDTCSGENFQIAAGDVTARVVECITLLPVNWEAATRFDSTHILDFGPGGVSGLGVLTHRNKEGTGLRIILAGALDVAVDDEYGCKQEIFTKSAGSIKWAPNWLNEYRPQLVKTAAGKIYVDTKFSRLLGRAPLMVPGMTPTTVNTEIVLATINAGYHIELAGGGYFHAKGMEAALAEVAAKIRPGCGIGINLIYVNPRMLQWGIPLIKNMRERGFPIQSLTIGAGVPSLEVASEYIETLGLTHLGLKPGSVDAIHAAIAIAKAHPTFPIVLQWTGGRGGGHHSFEDFHQPVLQTYSKIRKCANIVLVAG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The For acetyltransferase activity active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "EDCLYLNIYAPAHAETGSKLPVMVWFPGGAFETGSASIFDGSALASYENVLVVTIQYRLGIFGFFNTGDEHARGNWAFMDQVAALVWVQENIEFFGGDPRCVTIFGESAGAISVSSLILSPMTKGLFHKAIMASGVAIIPYLKASDYERNDDLQTIASICDCNASDSVALLQCLRAKSSEELLSISQKTKSFTRVVDGLFFPNELLDLLAQKLFHLVPSIIGVNNHECGFLLPMKEFPEILGGSNKSLALQLIHSVLHIPVQYSYLVADEYFHNKHSLLDIRNRFLDLLGDVFFVVPGLVTAQYHTDAGAPVYFYEFQHRPQCLKDRKPPFVKADHTDEIRFVFGGAFLKGNIVMFEEATEEEKALSRKMMRYWANFARTC",
    "response#2": "EDCLYLNIYAPAHAETGSKLPVMVWFPGGAFETGSASIFDGSALASYENVLVVTIQYRLGIFGFFNTGDEHARGNWAFMDQVAALVWVQENIEFFGGDPRCVTIFGESAGAISVSSLILSPMTKGLFHKAIMASGVAIIPYLKASDYERNDDLQTIASICDCNASDSVALLQCLRAKSSEELLSISQKTKSFTRVVDGLFFPNELLDLLAQKLFHLVPSIIGVNNHECGFLLPMKEFPEILGGSNKSLALQLIHSVLHIPVQYSYLVADEYFHNKHSLLDIRNRFLDLLGDVFFVVPGLVTAQYHTDAGAPVYFYEFQHRPQCLKDRKPPFVKADHTDEIRFVFGGAFLKGNIVMFEEATEEEKALSRKMMRYWANFARTCGQ",
    "response#3": "VLGTKWCGAGNEAANYSDLGYFNNVDRCCREHDHCDNIPAGETKYGLKNEGTYTMMNCKCEKAFDKCLSDISGYFTRKAVSAVKFTYFTLYGNGCYNR"
  },
  {
    "reference": "MAAAAVVVPAEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASSVLNDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGNITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPSGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEYKREIVEAKQIVRKLTMVVLSSEKLDERDKEKDKDDEKVEKPPDNQKLGLLEALLKVGDWQHAQNIMDQMPPYYAASHKLIALAICKLIHITVEPLYRRVGVPKGAKGSPVSALQNKRAPKQVESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNGHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKERMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must contain a signal peptide for proper functionality.2. The protein must exhibit the following characteristics: Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA; TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap- dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway; Plays a role for proper neuronal development.; Required for efficient export of polyadenylated RNA and spliced mRNA.3. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.4. The protein design should include a Nuclear localization signal motif to ensure proper function.5. A protein that can perform mRNA binding in nuclear speck, nucleus, chromosome, telomeric region to support RNA splicing, generation of neurons. The designed protein sequence is ",
    "response#1": "MCPERERVTRERLRDLAVFERLYGNPSKSSTEIAVKKFCRTLSAADVQASDVRPLPVLEETLRYLLSLLDSKEHPFEVVHDFIFDRTRSIRQDLSIQNLANERVIYLYEEMVKFHVISHERLQSCSGTSISSMHHLNMEQLAKTLTSLYNIYDANRKPDYIYENEAEFRSLYVLLHLNPSSGVMGEPLSLWFRKLTFALVKSKEICFVRNLLRLYRMGNYKNFLSRTASEATYLQYCISEHHIREMRLVAVQYINNVCYKLQPYPLLRLSQNLKMKELDVESLCHECGLESGLENHITNSYANSLLQLLRFTPILRNLALQHAATSCLDQHCLLCELGYLCDSMEKARGASCQATNMLKMLSQHPAAANLHLLDGDRIASSSAMKIQGLLRFLLDFMSRDYGKTSPGINDLENATATSSQSLIRCDQCKSETSRPQTNYVQDLAYSPVTSNGNKGVDVADSYLAAQQGVARGRMAPAKLPSFSQILKKSIEREQVTRGWCTTCRGYQPLSMRKIIKSIPQVLAANTCISSVEEKKLWATPGWLPEEVGFIIDKEHE",
    "response#2": "MCPERERVTRERLRDLAVFERLYGNPSKSSTEIAVKKFCRTLSAADVQASDVRPLPVLEETLRYLLSLLDSKEHPFEVVHDFIFDRTRSIRQDLSIQNLANERVIYLYEEMVKFHVISHERLQSCSGTSISSMHHLNMEQLAKTLTSLYNIYDANRKPDYIYENEAEFRSLYVLLHLNPSSGVMGEPLSLWFRKLTFALVKSKEICFVRNLLRLYRMGNYKNFLSRTASEATYLQYCISEHHIREMRLVAVQYINNVCYKLQPYPLLRLSQNLKMKELDVESLCHECGLEL",
    "response#3": "MCPERERVTRERLRDLAVFERLYGNPSKSSTEIAVKKFCRTLSAADVQASDVRPLPVLEETLRYLLSLLDSKEHPFEVVHDFIFDRTRSIRQDLSIQNLANERVIYLYEEMVKFHVISHERLQSCSGTSISSMHHLNMEQLAKTLTSLYNIYDANRKPDYIYENEAEFRSLYVLLHLNPSSGVMGEPLSLWFRKLTFALVKSKEICFVRNLLRLYRMGNYKNFLSRTASEATYLQYCISEHHIREMRLVAVQYINNVCYKLQPYPLLRLSQNLKMKELDVESLCHECGLESGLENHITNSYANSLLQLLRFTPILRNLALQHAATSCLDQHCLLCELGYLCDSMEKARGASCQATNMLKMLSQHPAAANLHLLDGDRIASSSAMKIQGLLRFLLDFMSRDYGKTSPGINDLENATATSSQSLIRCDQCKSETSRPQTNYVQDLAYSPVTSNGNKGVDVADSYLAAQQGVARGRMAPAKLPSFSQILKKSIEREQVTRGWCTTCRGYQPLSMRKIIKSIPQVLAANTCISSVEEKKLWATPGWLPEEVGFIIDKEEESSESEDEEWILLVSGVKEDAEEDDLYNTFSDFGHVKDLHLNLERRTGYAKGYALVEYESFEEAQTAIKAMNGTQLLTRTVYVDWAFSGLENHITNSYANSLLQLLRFTPILRNLALQHAATSCLDQHCLLCELGYLCDSMEKARGASCQATNMLKMLSQHPAAANLHLLDGDRIASSSAMKIQGLLRFLLDFMSRDYGKTSPGINDLENATATSSQSLIRCDQCKSETSRPQTNYVQDLAYSPVTSNGNKGVDVADSYLAAQQGVARGRMAPAKLPSFSQILKKSIEREQVTRGWCTTCRGYQPLSMRKIIKSIPQVLAANTCISSVEEKKLWATPGWLPEEVGFIIDKEH"
  },
  {
    "reference": "MPQQVYHSPVYHSQSIQAWEQRWFAKQNSSYGLMQQVAWKIAQRLLHIFQQRNIQNIAVCCGQGNNAGDGYLTAKYLKQYGFNVDIYAAELGTSKDLHSAYQEVVDVGIKIYPDFVFQKPYDCFIDALFGIGLNRELSDDWQQIIQTINAQTGLKIAIDIPSGIQANSGQPLTCAIKADYTYTALGLKAGLFTGQGKEFAGQVDVISLIPADAELKPIAQLSPHCISLPQREAFGHKGSYGHVLIIGGHADMGGAVMMAAESAFSAGAGKVTVVCHEKHHTAILARAPNIMLRDINALTKAEIQQLLDHVDAVSFGMGLGRDSWSEQQYFKWFPKINSSAHLHVVLDADALWYLAEHPALLKQDSYATPHPGEAAKLLGCSVKEVESDRIAAIHQLQEKYAGQWVLKGSGSLILEDQLWICTAGNAGMGTGGMGDVLAGMTASLKAQFAEKIHLHEIVTLHALAGDLLAQQGMRGVQAHHMHDAVYRVVNTGS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration..2. The protein must have high specificity towards metal ion binding, NADPHX epimerase activity.3. The protein should be able to bind (6S)-NADPHX, AMP ligand in a variety of conditions4. The protein should be designed to catalyze the following reaction (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.5. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.6. The designed protein should contain one or more YjeF N-terminal domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MKAIDKNAVGLGMHPLQLMENAGAAVADVISNHKRYSDSSVLFFAGLGNNGGDAFVAARHLANAQKSVILFLLGRETQIKTPESQINFKLMKQTNHVTVYEIQSESELSDAYGLFKENNLMIVDGIFGTGFSGKPKGLEKAAVQLINSEKEARQLPVLAIDIPSGMPPAGFSKDMNDLSDDTSDDLIIKADATITFHKMKTFLENKIAEKYAGEIFVRTIGIPENAGVARLLPVPGAESDKYRRGVVGIAAGSARYPGAAVLAVAGALRGGAGAVRYVGPAGHAVLARYPETLVSDQGPERAGRVQAWVVGPGAGDDAGTVAQVLAADVPVLIDADGLRLADVAAVRGRTAPTLMTPHAGEAAALLGVSREEVEAGRLAAVRELAARYGSAVLLKGSTTVVAGAGGGGAVRVNPTGTAWLATAGSGDVLSGLAGSLLAAGLSAVDAGSAGAYLHGLAGRFAAAGAPVGAHEVAEAVPGL",
    "response#2": "MAKYDGYAIKTWGIPSTVLMENAGRNTYRLMKEQYLAGAAAIAVVCGRGNNGGDGFVISRYALRDGFRTTVFLLGKRSELKGDAALNMELYASIGGEVVECTKDIDALRKGIQGADVLVDAIFGTGLSKATGGNEKLAIEAMNGSGKPVIAVDIPSGIDGKTGLPLGAAIRATHTFTYGYPKLGQILYPGAYHAGRLTVIDISIPASIEPDVHKGKMGHVLLVGSSVGKTGALIMSAKAATRTGSGLVSVGVPKGLNQIFEIALTEEMSIPLEGEDRLSEKSADRIIQMQDSFSAIGVGMGMGTYQEGESIVKKLIKDIKKPLLLDADAINNLANIKDLNILKEREYPTVLTPHVGEFARLSGYEKDSIVHNLIDVAQEFSQKWNCFLVLKSSRTVISTPDGYAFVSLRGSPAMAKGGSGDVLSGILTSLMGRGIPLLDALRLGVFLHGLAGEIAERKRHTESVRALDIVEEIR",
    "response#3": "MMDAAGRGLTQAIRNLATWSRRPDSVVRLLAGPGNNGGDAFAAALYLKEIGMHPEVWLACPKEKLRGPAKAFFEHMVQDGIPWREMVGEKAWASLSLEPLPPFILVDALLGTGAKGEPTGDIRRAVDYLRTRSPFSLVVAADIPTGMNADTGALAECAVQADVTVAMGFPKAREVLEKVKVFKREPDVHKGKMGHVLLVGSSVGKTGALIMSAKAATRTGSGLVSVGVPKGLNQIFEIALTEEMSIPLEGEDRLSEKSADRIIQMQDSFSAIGVGMGMGTYQEGESIVKKLIKDIKKPLLLDADAINNLANIKDLNILKEREYPTVLTPHVGEFARLSGYEKDSIVHNLIDVAQEFSQKWNCFLVLKSSRTVISTPDGYAFVSLRGSPAMAKGGSGDVLSGILTSLMGRGIPLLDALRLGVFLHGLAGEIAERKRHTESVRALDIVEEIPSAYNQVLLVGSSVGKTGALIMSAKAATRTGSGLVSVGVPKGLNQIFEIALTEEMSIPLEGEDRLSEKSADRIIQMQDSFSAIGVGMGMGTYQEGESIVKKLIKDIKKPLLLDADAINNLANIKDLNILKEREYPTVLTPHVGEFARLSGYEKDSIVHNLIDVAQEFSQKWNCFLVLKSSRTVISTPDGYAFVSLRGSPAMAKGGSGDVLSGILTSLMGRGIPLLDALRLGVFLHGLAGEIAERKRHTESVRALDIVEEIL"
  },
  {
    "reference": "MTPLIFVTGGVVSSLGKGIAAASLASILEARGLSVTMMKLDPYINVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFVHTRLTGKNSITTGKIYESVIRKERRGDYLGATVQVIPHITDEIKHCVHEATRGFDVALVEIGGTVGDIESLPFLEAIRQLRIEHGPEKCLFMHLTLVPYIKAAGEIKTKPTQHSVKELRSIGIQPDILLCRCEEALPESERRKIALFTNVPENAVISAIDVDVIYKTPLWLHSQGLDDIVVRRLGLDAKPADLSSWRRTVEAVEHPKDEVTIAIVGKYVEHKDAYKSLGEALRHGGIKQQTRVNLDWIDSEEVESRGAQTVLGKADAILVPGGFGKRGFEGKVLSAKYARENGVPYFGICYGMHAAVVDFARNVAGLQDADSSENDRNTANPVIALITEWTTAAGETEQRSEHSDLGGTMRLGSQEARLKTGSLARETYGHEVVRERHRHRYEFNNRYRQDFEDLGLVVSGKSMDDLLVEIVELPQQKHPWFLACQAHPEFTSTPRDGHPLFIGFVEAAREYKAMRQGEKLAGAQARA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that is capable of catalyzing reactions within the CTP from UDP: step 2/2 pathway with high efficiency and specificity.2. The protein design must be focused on enhancing the efficiency of 'de novo' CTP biosynthetic process.3. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen4. The designed protein must have a Nucleophile; for glutamine hydrolysis that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "LPDAYLSVTEAMRAGGFQNDAKVHIRWVASDECQTEAGAQRALGGVDAVLVPGGFGVRGIEGKLGALRWAREKQVPTLGICLGLQCMVIEYARNVAGIEGASSTEFDPQTPAPVIATMEEQKAFVEGAGDLGGTMRLGAYPAKLAEGSVIAEAYGAVAVDERHRHRYEVNNGYRKELEEAGLVFSGLSPDGNLVEFVELPREVHPYYVSTQAHPEFKSRPHRAHPLFAGLVAIPDAYKSVYEAISHGGVANDCGVEIERIEAEQIEKEGPEKLLKGLGGILVPGGFGERGVEGKIMAARYARENKVPYLGLCLGMQVATVEFARNVLGLKGAHSTEFNKETPHPVIAMLEEQKGIKNLGGTMRLGAQPVQFKMGSRAARLYGAFMASERHRHRYEFNNAYREQFEKAGFICSGTSPNGELVELIELQDHPFFVGCQFHPEFQSKPNAPHPLFRGFIAVRTL",
    "response#2": "LKDSYKSLIEALAHGGIANEARVDIQWVDAEHVEKMGADAPFADVHGILVPGGFGDRGIEGKVQAVRYARERGVPYFGICLGMQCAVIEYARSVCGLAGANSTEFEPHPAHNLIDLLPEQRTIADKGGTMRLGLYPIVLGEGSLASRLYGQGIIQERHRHRYEVNNDYVHRLEKHGLRISGIWAEKQLVEIIEIPEHPYFVAGQFHPEFRSRPWDPHPLFAGFIR",
    "response#3": "LPDAYLSVTEALRAGGFAHRAKVNLRWVPSDDCETPEGAARALDGVDAVCVPGGFGVRGVDGKVGALRHAREHGIPTLGLCLGMQSMVIEFARNVLGWADAHSTEFDADTAHPVVATMAEQRDIVSGEGDLGGTMRLGSYPAVLAPGSVTAEAYGTTEVTERHRHRYEVANDVRADLEAAGLVISGTSPEGALVEFVELPRDVHPYYVATQAHPEFKSRPTQAHPLFAGLLAAVGAL"
  },
  {
    "reference": "MIKAFLIERRSWIAAFLFQQALMLFIAFVDPSISFGNVLYMVYLCILFFIIFLWFRYRKETAFYKSLKTWENNLDVTAINEPETPFEAMVERSIAGQTEHLKQTAARHRLALENEKDELMAWIHEVKTPLTAMHLIIDRMEEKALKSQLSYEWLRIHLLLDQQLHQKRISFIENDLSVEFIQLQPLIFKEIKDLQSWCIQKGIGFDIQLEAKEVLSDAKWLAFIIRQLLTNAVKYSEASEIEIKSFQKGEQTQLQVKDCGRGIDPKDVPRIFDKGFTSTTDHHDQASTGMGLYLAKKAAAPLLIHIDVESEFGAGTVFTLTFPIRNQFEHVISV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Extracellular topological domain of the protein should be designed to be flexible or rigid.2. The protein can be targeted to plasma membrane and perform ATP binding.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.4. The protein have enhanced ATP + protein L-histidine = ADP + protein N-phospho-L-   histidine.. The designed protein sequence is ",
    "response#1": "KGGKVGLFGGAGVGKTVNMMELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVSLVYGQMNEPPGNRLRVALTGLTMAEKFRDEGRDVLLFIDNIYRYTLAGVEVSALLGRMPSAVGYQPTLAEEMGMLQERITSTKTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRQIGS",
    "response#2": "LDCGLASNESPYNEANSNLTYISDADFIQGGKTGNVQKDLLMKLRKPYTVLRYFPDGIRNCYSLNVKQDTNYLIRVMFRYGNYDGLNNSPRFDLYLGPNIWTTIDMGKSGDGVLEEIIHITRSNILDICLVKTGTSTPMISSIELRPLLYDTYIAQTGSLRNYNRFYFTDSNNYIRYPQDVHDRIWVPLILPEWTHINTSHHVIDSIDGYDPPQDVLRTGAMPANASDPMTITWNLKTATDQVYGYIYIAEIMEVQANETREFEVVVNNKVHFDPFRPTRFEAQVMFNNVPLTCEGGFCRLQLIKTPKSTLPPLMNAFEIFS",
    "response#3": "LDCGLASNESPYNEANSNLTYISDADFIQGGKTGNVQKDLLMKLRKPYTVLRYFPDGIRNCYSLNVKQDTNYLIRVMFRYGNYDGLNNSPRFDLYLGPNIWTTIDMGKSGDGVLEEIIHITRSNILDICLVKTGTSTPMISSIELRPLLYDTYIAQTGSLRNYNRFYFTDSNNYIRYPQDVHDRIWVPLILPEWTHINTSHHVIDSIDGYDPPQDVLRTGAMPANASDPMTITWNLKTATDQVYGYIYIAEIMEVQANETREFEVVVNNKVHFDPFRPTRFEAQVMFNNVPLTCEGGFCRLQLIKTPKSTLPPLMNAFEIFS"
  },
  {
    "reference": "MARPSVCLLASLSALLLGIAAVSRSKGLRGTESQREPRIPSQFSQEQRIAMKEALKGAIQIPTVSFSPKELNTTALAEFGEYIRKVFPTVFHTSFIRHEVVGNYSHLFTIKGSDPSMQPYILLAHIDVVPAPDKGWDVPPFSGLERDGFIYGRGTLDNKNYLMAILQALELLLIRNYIPRRSFFIALGHDEEISGINGAQKISALLQARGVQLAFVVDEGSFILDGFIPYLKKPFAMVSVSEKGAINLMLQVNTTTGHSSAPPKETSIGILAAAVSRLEQTPMPNMFGSGPLMTAVEQLANEFPFPTNIVLNNLWLFRPLVSRLMERNYITNSLVRTTTALTMFNAGVKVNVIPPVAEAIINFRLHPAQTVQEVLKLAKDIVADDRIQFHVLDAFDPLPISPSDDQALGYQLLRQTIHSVFPEVNIVAPGTCIGNTDSRHYLNLTTGIYRFNPIYLQPQDFSSIHGINEKISVQAYETQVKFVFEFIQNGDTDEETVPHLHEL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The presence of Zn(2+) is necessary for the protein to function in the desired environment.2. For general function, the protein need meet that Some of these NAAs stimulate oxidative metabolism via mitochondrial uncoupling, increasing energy expenditure in a UPC1-independent manner; PM20D1 circulates in tight association with both low- and high-density (LDL and HDL,respectively) lipoprotein particles.3. The designed protein must possess hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides, peptidase activity, lyase activity to support respiratory electron transport chain, energy homeostasis, amide biosynthetic process under certain conditions.4. The Proton acceptor of the protein must be engineered to enable effective catalysis of (9Z)-octadecenoate + L-phenylalanine = H2O + N-(9Z-   octadecenoyl)-L-phenylalanine.5. Ideal protein should have a binding affinity for key enzymes in the fatty acid metabolism pathway to enhance the overall efficiency of the pathway.6. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "MSEKTWLYYEMSPYKMMTYQELIWVMHDMGRGLAKIGIKPNGEHKFHIFASTSHKWMKIFLGCISQGIPVVTAYDTLGESGLIHSMVETESAAIFTDNQLLAKMIVPLQSAKDIKFLIHNEPIDPNDRRQNGKLYKAAKDAINKIREVRPDIKIYSFEEVVKIGKKSKDEVKLHPPEPKDLACIMYTSGSISAPKGVVLTHYNIVSGIAGVGHNVFGWIGSTDRVLSFLPLAHIFELVFEFEAFYWNGILGYGSVKTLTNTSTRNCKGDLVEFKPTIMIGVAAVWETVRKAILEKISDLTPVLQKIFWSAYSMKEKSVPCTGFLSRMVFKKVRQATGGHLKYIMNGGSAISIDAQKFFSIVLCPMIIGYGLTETVANACVLEPDHFEYGIVGDLVGSVTAKLVDVKDLGYYAKNNQGELLLKGAPVCSEYWNKPEETKKSFKDNWYHTGDVGVLDEDGFCSITGRIKDTVIVHGVSYGIPELETFLQTHEKVDHVYLVGQKDPQAGEETEGFVVFYAPTFELTLDDVKQQLREIGKEKIVLLVIPKELIKVPKTSVGKIARARLKEQYLAGELKKVK",
    "response#2": "MSLEKTWLYYEMSPYKMMTYQELIWVMHDMGRGLAKIGIKPNGEHKFHIFASTSHKWMKIFLGCISQGIPVVTAYDTLGESGLIHSMVETESAAIFTDNQLLAKMIVPLQSAKDIKFLIHNEPIDPNDRRQNGKLYKAAKDAINKIREVRPDIKIYSFEEVVKIGKKSKDEVKLHPPEPKDLACIMYTSGSISAPKGVVLTHYNIVSGIAGVGHNVFGWIGSTDRVLSFLPLAHIFELVFEFEAFYWNGILGYGSVKTLTNTSTRNCKGDLVEFKPTIMIGVAAVWETVRKAILEKISDLTPVLQKIFWSAYSMKEKSVPCTGFLSRMVFKKVRQATGGHLKYIMNGGSAISIDAQKFFSIVLCPMIIGYGLTETVANACVLEPDHFEYGIVGDLVGSVTAKLVDVKDLGYYAKNNQGELLLKGAPVCSEYWGKPEENAKTFRADRWFPTGDLAYQDEEGFFYIADRKKDMIISGGENIYPA",
    "response#3": "MTEKTWLYYEMSPYKMMTYQELIWVMHDMGRGLAKIGIKPNGEHKFHIFASTSHKWMKIFLGCISQGIPVVTAYDTLGESGLIHSMVETESAAIFTDNQLLAKMIVPLQSAKDIKFLIHNEPIDPNDRRQNGKLYKAAKDAINKIREVRPDIKIYSFEEVVKIGKKSKDEVKLHPPEPKDLACIMYTSGSISAPKGVVLTHYNIVSGIAGVGHNVFGWIGSTDRVLSFLPLAHIFELVFEFEAFYWNGILGYGSVKTLTNTSTRNCKGDLVEFKPTIMIGVAAVWETVRKAILEKISDLTPVLQKIFWSAYSMKEKSVPCTGFLSRMVFKKVRQATGGHLKYIMNGGSAISIDAQKFFSIVLCPMIIGYGLTETVANACVLEPDHFEYGIVGDLVGSVTAKLVDVKDLGYYAKNNQGELLLKGAPVCSEYLGNPEKTAEAFKHFETISYGELWDRISALADVLSTEQTVKPGDRVCLLGFNSVDYATIDMTLARLGAVAVPLQTSAAITQLQPIVAETQPTMIAASVDALADATELALSGQTATRVLVFDHHRQVDAHRAAVESARERLAGSAVVETLAEAIARGDVPRGASAGSAPGTDVSDDSLALLIYTSGSTGAPKGAMYPRRNVATFWRKRTWFEGGYEPSITLNFMPMSHVMGRQILYGTLCNGGTAYFVAKSDLSTLFEDLALVRPTELTFVPRVWDMVFDEFQSEVDRRLVDGADRVALEAQVKAEIRNDVLGGRYTSALTGSAPISDEMKAWVEELLDMHLVEGYGSTEAGMILIDGAIRRPAVLDYKLVDVPDLGYFLTDRPHPRGELLVKTDSLFPGY"
  },
  {
    "reference": "MSSFNKKIAKDIKKIKISLAAQDDVKNWSCGEVTKPETINYKSYKPERDGLFDELIFGPTTDFKCPICGTKYKKSDENTICEKTPMCQKYQPVILPKMVRRSRMGHIHLENPVVHFWFFKIDHSIISKLLGLRIANSNKTVSKGDLEKLIYYKSHIVLEDGGLKKLQKNAIIDISDAAIIYADALEELKERFEKDTEEYEIISEALAELESYAVSQTGQDYGIDFYEYNEIIHEFSKAKISTGSKAIEYLLENIDLKAEAEFIESEIEKINQHFDNNPTASIKAQERSKLYKRLAVVNAFIHSGQDPKSMLIYDLPVIPADLRPLVQLDGGRHSTSDVNELYRRIIIRNNRLKKWEETDAPMLIKQNEFRMIQEAVDALIDNSRKKPAPVTSKDNRPLKSISDTLTRKKGRFRQNLLGKRVDYSGRSVIVVGPELKMHQVGVPREMAAKLFEPWIIKELINGDSHINSIKAGKKAIENLDPIIWPYVEKAIEGKVVLLNRAPTLHRLSIQAYEPVLIRGKAIKLHPLSCTPFNADFDGDQMAIHVPISEEAVREARELMLASKNILGPKDGEPIINPAQDIILGLYYLTMEKAAAIDGDKVVGEGKFFATYDEMLLAYENKLVSLHARIALPISEINKKRLNYSPENKYIISTVGKFIFNRAFPESFEFIFGKHVEYVNKTDANGEVKLSEKEVVYTSNNKNQLEQFLLPYGQNFAEVIAKMPLNLPLSKKDVAKIVRRVYDKYVAIVTKSDVASVINQINANEINQI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have DNA-directed 5'-3' RNA polymerase activity, zinc ion binding within DNA-directed RNA polymerase complex to regulate DNA-templated transcription.2. The Zn(2+), Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.3. The stability of the protein-Zn(2+) complex should be optimized to maximize enzymatic activity.4. For general function, the protein need meet that DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The designed protein sequence is ",
    "response#1": "MWMILDVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPDIIVRNEKRMLQEAVDALVDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPNLKMHQCGLPKEMALELFKPFVMKKLVDKAIASNIKSAKKMVERGREEVWDILDDVIKEHPVLLNRAPTLHRLGIQAFEPVLVEGRALQLHPLVCTAYNADFDGDQMAVHVPLSAEAQTEARLLMLASNNILNPKDGKPVVTPTQDMVIGLYYLLAAQNILNPKDGKPVVTPTQDMVIGLYYLTFISAEAFSKENPRAFSSTDEARMAFELGQIDLHEKIRVKMSVPQKTGREILKSHEAQTFLEEIVDTSVGRIIFNDAIPTSLGFYNEIIDKKKLGNIVYECYRLEGNARTADMLDSLKSLGYEFSTRAGITVGVTVPTKKFDLGMVLDAVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPEIIVNNEKRMLQEAVDSLFDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKQMALELFKPFVMKRLVDLNHAQNIKSAKRMVERYRPQVWDVLEEIITEHPVLLNRAPTLHRLGIQAFEPMLVEGKAIQLHPLVCGAFNADFDGDQMAVHLPLSPEAQAEARVLMLSSNNILKPSDGRPVTLPSQDMIIGLYHLMSTNNILSPANGKPIIVPSQDMVLGIYYLSMDRAGEPGEGMMLADMAEVHQALEAKAVTLHSKIVARVPQTDEDGNQYLKRFETTPGRMLIGECLPKSHKVPFEIVNRLLTKKEIGDVIDQVYRHTGQKDTVLFADAIMALGFRHAFKAGISFGKDDMVVPPNKKTLVEGAETEVQEYNQRYQDGISDEERYEQVISIWNETKDKLTEELIQNLDEFNPIFMMASSGARGSKNQISQLAGMRGLMANPSGKTIEMPIKSNF",
    "response#2": "LGMVLDAVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPEIIVNNEKRMLQEAVDSLFDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKQMALELFKPFVMKRLVDLNHAQNIKSAKRMVERYRPQVWDVLEEIITEHPVLLNRAPTLHRLGIQAFEPMLVEGKAIQLHPLVCGAFNADFDGDQMAVHLPLSPEAQAEARVLMLSSNNILKPSDGRPVTLPSQDMIIGLYHLL",
    "response#3": "EWMILKNIVVTPPDTRPIIQLEGGKFTTSDINSFYRKIIIRNERLKRVIQNNAPSIILNNEKRLLQEAVDALFDNASRKKPLLGKDKRPLKSLSEHLKGKQGLFRQNLLGKRVDYSGRSVIVIGPELKMYQVGIPVSMILKLFKPFIIHELIKKTDDEGNTKDPIVSNIKSAEKLILNQDNTIWPIINKVIKQRPVLLNRAPTLHRLGIQAFEPILVEGKAIRLHPLVTTAFNADFDGDQMAVHVPLSPEAVAEARTIILASWHILGPKDGKPIITPTQDMVLGNYYLL"
  },
  {
    "reference": "MITPTSFEPVELLYSKKKVNRTNWKKNVLKSNPEINNLCERIYPINRAIQFGKQFHPGKAEVKQKLDKTAIIQLILQHLSTKGLKQTKQTLEKEARTTTPIVEGLNESRLVTYIRNALKDTDRIYDLSMEHTEYSKEERQSKITEREELLFQMDLLEDEDEDDGVNIWDEPTENIITEKVHTTEYDINKSKENKDDQDDEVVKFASLNKLVEHLTHDSKHDLQFLKTFLMTYQSFCTPEKLMSKLQQRYNCPSGHDEMATRNIQIRVINVLKGWVDNYYSDFDDKLIAMLRTFIDQIQIKFPAPASAVNKSLTKMVEKLSPVNDSKHIFNEKTPEPMVPKNIFSNNLSIYDIDEEEIARQLTLIEFEIYRNIKPPELLNQSWNKTKLKSRAPNVLKMIDRFNSVSMWVATMIIQTTKVKARARMMTRFIKIADHLKNLNNYNSLMAIIAGLNFSSVYRLKYTREELSAQTMRTYSDLEKIMNSEGSFKTYRTRLQNVPPMLPYLGVHLTDLTFIDENPNNFVTDVGGKQVSLINFTKRTLVFKIISLIQETQVVPYNLQPVHQIQEFLLNIRSDLKAHTLDQYQQELYRESLKREPKKAQRSDVL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Ras-bound GDP/GTP exchange factor required for normal activation of adenylyl cyclase; Component of the Sca1 complex, a regulator of cell motility, chemotaxis and signal relay; The Sca1 complex is recruited to the plasma membrane in a chemoattractant- and F-actin- dependent manner and is enriched at the leading edge of chemotaxing cells where it regulates F-actin dynamics and signal relay by controlling the activation of rasC and the downstream target of rapamycin complex 2 (TORC2)-Akt/protein kinase B (PKB) pathway..2. The designed protein must have at least one N-terminal Ras-GEF, Ras-GEF, LisH domain.3. A protein that contributes to chemotaxis to cAMP, response to electrical stimulus, positive regulation of gene expression, aggregation involved in sorocarp development, sorocarp development by functioning in Ras guanyl-nucleotide exchange factor complex, cytosol, plasma membrane with guanylate cyclase activator activity, protein kinase inhibitor activity, protein kinase activator activity, guanyl-nucleotide exchange factor activity. The designed protein sequence is ",
    "response#1": "QDGHLISGSLEALMEHLVPTVDYYPDRTYIFTFLLSSRVFMPPHDLLARVGQICVEQKQQLEAGPEKAKLKSFSAKIVQLLKEWTEAFPYDFQDEKAMAELKAITHRVTQCDEENGTVKKAIAQMTQSLQYRVEHLTTFVLDRKDAMITVDDGIRKLKLLDAKGKVWTQDMILQVDDRAVSLIDLESKNELENFPLNTIQHCQAVMHSCSYDSVLALVCKEPTQNKPDLHLFQCDEVKANLISEDIESAISDSKGGKQKGILTCIECSGIHREMGVDRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTALGNTVLHYCSMYSKPECLKLLLRSKPTVCSLPESTIKEITDKMKELLISESNVQEIRSPVTVVGDVHGQFYDVLEIFKIGGQCPDTNYLFLGDYVDRGYHSVETISLLTCLKLRYPSRITLLRGNHESRQITQVYGFYGECMRKYGNPTVWKYFTEMFDYLSVAAIIDEAIYCVHGGLSPSALSIDQIKVLDRFQEVPNEGALSDILWSDPDPDREGFVESQRGAGYSYGKDVTLRFLQNNKMQHIIRAHQLCMDGYQTLFDNKLSTVWSAPNYCNRCGNMASIVEVNEKLERYFNTYAAA",
    "response#2": "EIARQLTLIEFEIYRNIKPPELLNQSWNKTKLKSRAPNVLKMIDRFNSVSMWVATMIIQTTKVKARARMMTRFIKIADHLKNLNNYNSLMAIIAGLNFSSVYRLKYTREELSAQTMRTYSDLEKIMNSEGSFKTYRTRLQNVPPMLPYLGVHLTDLTFIDENPNNFVTDVGGKQVSLINFTKRC",
    "response#3": "DPLVLAQQLTHIELDRVSSIYPEDLMQIVSHMDSLDNHRCRGDLTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQMANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFVASFRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQRDVQILNHILDDIEFFITKLQKAAEAFSELSKRKKSKKSKRKGPGEGVLTLRAKPPPPDEFVDCFQKFKHGFNLLAKLKSHIQNPSASDLVHFLFTPLNMVVQATGGPELASSVLSPLLTKDTVDFLNYTVKAEERQLWMSLGETWMKVRAEWREMHGPGERDVQILNHILDDIEFFITKLQKAAEAFSELSKRKKSKKSKRKGPGEGVLTLRAKPPPPDEFVDCFQKFKHGFNLLAKLKSHIQNPSASDLVHFLFTPLNMVVQATGGPELASSVLSPLLTKDTVDFLNYTVKAEERQLWMSLGETWMKVRAEW"
  },
  {
    "reference": "MKAKLVSLLAQANIKISDQQIQQLINLVNLLNKWNKAYNLTSVRDPQEMLVKHILDSLVVSPYLQGDRFIDVGTGPGLPGLPLAIINPSKQFVLLDSLGKRISFIRNAIRELRLTNVTPVLSRVEEYQPEDKFDGVLSRAFASLKDMTDWCHHLPKENGYFYALKGIYQEDEINELNKKYTIQKVIELSVPELIGERHLIVLR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. We are looking for a protein with broad substrate specificity for guanosine(527) in 16S rRNA + S-adenosyl-L-methionine = N(7)-   methylguanosine(527) in 16S rRNA + S-adenosyl-L-homocysteine.2. The designed protein should be able to compete with endogenous proteins or inhibitors for S-adenosyl-L-methionine binding.3. For general function, the protein need meet that Specifically methylates the N7 position of guanine in position 527 of 16S rRNA.4. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "MELTICALPGVFSQTHLDVGTAVLLPYLNQVKSGRIADFGCGAGIISCYLAKANSSNIIHALDIDAFALQSTEMTFSRNGIGSDQLRLQPVTGIADAPTELDAIVSNPPFHQGIHTNYDASEGLCQNAKKHLKASGELWIVANRFLNYPILIEKHFGQCEIKTDLQGFKVY",
    "response#2": "ELTICALPGVFSQTHLDVGTAVLLPYLNQVKSGRIADFGCGAGIISCYLAKANSSNIIHALDIDAFALQSTEMTFSRNGIGSDQLRLQPVTGIADAPTELDAIVSNPPFHQGIHTNYDASEGLCQNAKKHLKASGELWIVANRFLNYPILIEKHFGQCEIKTDLQGFKVL",
    "response#3": "ELTICALPGVFSQTHLDVGTAVLLPYLNQVKSGRIADFGCGAGIISCYLAKANSSNIIHALDIDAFALQSTEMTFSRNGIGSDQLRLQPVTGIADAPTELDAIVSNPPFHQGIHTNYDASEGLCQNAKKHLKASGELWIVANRFLNYPILIEKHFGQCEIKTDLQGFKVK"
  },
  {
    "reference": "MADLETSDLSRVLPSSNLLSLEQLQEQLKRHRDELFQQQQDSHVLGSKPDDSLNNNYLLSHQYHDSQVYIPSNTWSFTQGLIVGQLSVVFVIVIFIKFFVFAESSPALAKSSITKDASVVIVKRDKKDQSSSDDADPDDDSETTASNAKVAAILEKTYYDVDNHSPESLDWFNVLVAQTIAQLRSEALLSDNIYHSLNNFLLKSELPEYLDKINLTEIDIGDDFPIFSNCRIKHSKDGSGRLEAKIDVDLSDTLTLGIETKLLLNHPRPLTAVLPVQLSVSMVRFSGCLTVSLINTADPEFAELSAHNSPEPGEPMSRSHSAGSPGGDSSHDEVISTPDSSSHTAQRKHSKDDPNDGTALMFSFSPDYRLEFTVKSLIGARAKLQDVPKISSLIENRLRAWFIERCIEPRFQVVRLPSLWPRRKNTREQVTNKNGDKVEDGSN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.2. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.3. For general function, the protein need meet that Essential for establishing and maintaining the structure of mitochondria and maintenance of mtDNA nucleoids.; The MDM12-MMM1 subcomplex functions in the major beta-barrel assembly pathway that is responsible for biogenesis of all outer membrane beta- barrel proteins, and acts in a late step after the SAM complex; The MDM10-MDM12-MMM1 subcomplex further acts in the TOM40-specific pathway after the action of the MDM12-MMM1 complex4. The presence of SMP-LTD domains is critical for the interaction of the designed protein with its intended targets.5. The protein should be designed to localize specifically to endoplasmic reticulum membrane in order to perform its role in protein insertion into mitochondrial outer membrane more effectively. The designed protein sequence is ",
    "response#1": "KRPEDLQVLCRLRYNGNIRLSLTALVLLDYPMPNFVGLPLKLNITGLTFDGVAVVAYIRKRVHFCFLSPEDADTLFGADDTNEAGHLQSRDSLAGGINDTSYSSRRPHDSLLRDVRVESEIGRKEDGKQVLKNVGKVEKFVLEQVRRIFEEEFVYPSG",
    "response#2": "MSIDVNWRFATSGPDGEALAERIRSFIHDRFQQVALPRFIRSVQVHAFDFGTIPPELEIKDFCEPFADFYEEDDDDHTSDASEERGEEHSSRWNSTHPELNEPSYREDTAVNHSLRDPFPDGFPTSPLRSPLGEHLNPHFLPRASTPGIPGGTSTLGYHLMSLGGLSGTQTPLAAVAGGNPFASGWSDSGMGPGNRGRSETHAGMQHPRAEPEIDTSNSTSRPSTANTLPSHPSGSSKNSGQAATGRNDHPSLHAGEHLEDSVTQGQLPLPPRMRERRPEDFQVLCHVKYAGDVRLSLTAEILLDYPMPSFVGLPLKLNVTGITFDGVAVIAYIRKRVHFCFLSAEDADALIGPEQQQQRESAGDDHRPQSRPDSSASASQKRHGGLLQEIRVESEIGRKEDGKQVLKNVGKVERFVLAQVRRIFEEELVYPSFWTFLV",
    "response#3": "MSIDINWEAATSGPDGEALAERIRSFIHDKFQQIALPRFIRSVEVNSFDFGTIKPELQIKDLCDPFEDFYEEDEDNEIGDGEVSDIQDRSPKPRPSSAGNERSAADAWQAEHPAFLDDRLCGRIEPHDVPIPSKEDPLASRPIRSPMSFGDPLNPYFFPRAGTPGIPGGTSNLGYYYMPLGGISGTQTPLSSVPRGPFSPGLRDASVFGEPSNSQRPNPSGPARRQSEIDIDTGNSRPSTADTLDSLNIHGIPDPVLPRSSDDAHPNVLPRRDNSPAPRRVREKRPEDLQVLCRLRYNGNIRLSLTALVLLDYPMPNFVGLPLKLNITGLTFDGVAVVAYIRKRVHFCFLSPEDADTLFGADDTNEAGHLQSRDSLAGGINDTSYSSRRPHDSLLRDVRVESEIGRKEDGKQVLKNVGKVEKFVLEQVRRIFEEEFVYPSFWTFLN"
  },
  {
    "reference": "MNPWINADVLMVKKWTKNLFSLILNAPIEPFFAGQFNKLALYNSNPLNKNKIQRAYSYVNAPSEKNLEIYIVRVLNGQLSNLLYNLHSGDKIFIKKKSFGFFIIDEIPDCEILWMFATGTGIGPYCSILQEGKNINRFNHIILTHAVRYQNELTYLPLMKELRQKYNGKLQIQTITSREKHKNSLNGRIPFLLRNKILEKHVGFSINPQTSHVMLCGNPFMVKDTFLFLKNNRNMEKHLRRKKGHITMENYW",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein can be targeted to cytoplasm and perform nucleotide binding.2. The protein should be able to bind FAD ligand in a variety of conditions3. We need a protein that can perform NADP(+) + reduced [flavodoxin] = 2 H(+) + NADPH + oxidized   [flavodoxin] with high efficiency. The designed protein sequence is ",
    "response#1": "LQPFLAPVVSNQRVTGPSHFQDVRLIDFDITGSGISFAAGDVVLIQPSNSAAHVQQFCQVLGLDPDRCFTLQPREAGVPCPPGLPQPCSVQHLVTQYLDIASVPRRSFFELLACLSAHELEREKLLEFSSAQGQEELHEYCSRPRRTVLEVLCDFPHTAGAIPPDYLLDLIPAIRPRAFSIASSLLAHPSRLQILVAVVQYQTRLREPRRGLCSSWLASLDPGRGAVRVPLWVRPGGLAFPR",
    "response#2": "PNVNSAVVVSNKRVTPSDHWQDVRLIELDVTFPLVNGMAYEPLPGDRVVVYPKNYPSDVQKLIDLMEWGSVADKQISLGAQSPNGLYPTKNSTLRDLLTHNIDFMAVPSRSFLHKLAFYTTNVDHKEKLFELVQPDASQEFYDFTSRPRRTILEVLAEFYSSKIPYEAVPDVFPIIRGREFSIANGGKHWKKRQVIQTKHETSGGDAHQDGAVVYRLDVLAALVEYRTIIRKPREGLCSRYLKNLPAGTPLHVDMKRETPPPNGSIYAMVGPGTGVAPFRAAIQERVAQGRTGNVLFFGCRWRDQDFYWEAEWQELERKGCLTLVTAFSREQERKVYVQHRLRELGPLVWELLDRRGACFYLAGNAKYML",
    "response#3": "LQPFLAPVVSNQRVTGPSHFQDVRLIDFDITGSGISFAAGDVVLIQPSNSAAHVQQFCQVLGLDPDRCFTLQPREAGVPCPPGLPQPCSVQHLVTQYLDIASVPRRSFFELLACLSAHELEREKLLEFSSAQGQEELHEYCSRPRRTVLEVLCDFPHTAGAIPPDYLLDLIPAIRPRAFSIASSLLAHPSRLQILVAVVQYQTRLREPRRGLCSSWLASLDPGRGAVRVPLWVRPGGLAFPA"
  },
  {
    "reference": "MTESSEYLPEVVYGAEQVAALDRRFIQEFDVDGFELMQRAARAAYEELAHIWAMPGTLCVFCGPGNNGADGLLVADLAREGGWEVHAISLVGTEQYSGDAAKALSRYMDKGGTVENFSNATIEADVVVDALLGTGLNRDVEGDFAAAIERINEAGDAGIGVFAIDIASGIDASTGHVWAHAVRADATATFIGLKLGMLTGEGPAHCGDIAFSALDAPDALYFEQPYVARRLTHRRLRKALRPRSRNQHKGDNGHVLCIGGNRGMGGSIRMSAEAALRSGAGLVSVVCHADHAGAMSQARPELMCLGVSEQESISTLIEACDVIAIGPGLGSDAWAENLWHQVMACDKPLVVDADALNRLAREPFERDNWILTPHPGEAGRLLDWPTPEVLDDRVKAVQTLRERYGGSVVLKGAGSLIATPEELWLCTDGNPGMAVGGMGDLLTGVIAALAAQGLDTSAAASLGVYVHARAGDQAAAAAGQRGMLPTDLFDAIRSFVNPGRS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must bind to Mg(2+) in order to perform its enzymatic function.2. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration3. The designed protein should contain one or more YjeF N-terminal domains that are essential for its function.4. The protein should have a fast turnover rate for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.5. The K(+) binding site should be located in a region of the protein that is accessible to the ligand.6. A protein with ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding, NADPHX epimerase activity functions is required to enable nicotinamide nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "MREVDRLTTERYGIPSLQLMEAAGRRVADRVLGEFSPRLPQRVAVLCGKGNNGGDGLVAARLLHGKAAQVRVLLFGAGEELRGDAKTNLQRWRETGNEITRIESEAAWQSAWPELAGVDVIVDALLGTGLRGPTTGLIARGIEEINRFSNNATAPRPALILAVDTPSGLPCDGLHAEGPILRAHRTVTFTAPKVGQLISANSPAVGSLEVRSIGSPSVLIEPEEFSGLPLVRAVDAHKGSFGHVLLIGGSVGKSGALALAGRAALFAGAGLVTLASPRQLLPAIAASQAEYMTFPLAETAAGTISYENLHAPAFGFMLDDKSVLGVGPGVDVHEDTQKFVRELVQRTERPVVLDADGLNAFLFPDKLLRERKTKFLALTPHPGEMARLLGTTSAKVQEDRLNVALKSASRWDAHVVLKGFHTIVAAPDGSVFVNTTGNAGLAKGGSGDVLTGILAALTAQLGTEDWLRVLVLGVYLHGLAAELATKGIDLSGLLAGDVAVAVPQARLKLLEPALWLPEYPWPTVEGHKYLRGHAVVASGPIYSTGAARLAARGALRAGAGLVTVASPRDALAVNAAQLTAIMVREADDARGLAALLADTRKNAVLIGPGHGVGEGTRAMVLAALAAGPRVVLDADALTSFADDPETLFKAIHASEAPVVMTPHEGEFARLFGDLIAEPKVERTRKAAARAGATVLLKGADTVIAAADGQAAINGTASPWLATAGSGDVLGGIVLGLLAQGMTGFLGASAAVWMHGEAAKTFGPGLIAEDLPEMLPAVLRGLE",
    "response#2": "MNNIDKFCIETLGISGVILMENAALKVVSSISKDYDNVVIVCGTGNNGGDGMVCARHFKAKGKKIRVFYVGNIEKMSHDCKINYNILINMGINVTKINSEDDTKEFITYIKEADIVVDAVFGTGLNRQVKGIYSKVISCINEEAKYIVAVDVPSGMNGDTGAIMGNCIKANKTISFEMYKKGFLSYNNEQYTGEIVIENIGVPESILKESNKSSYGKVIIIGGSKEFIGAPLLSALGAIRAGTGRVYMAGPRDIVKSAVNYEPGIIPIAIENEYFSENDVNIVNNYIDSDTVVVIGPGLGRKNGVGLFVKKIVGSVNVPIIIDADAIYHISNLKDKIKDKDNLVITPHPGEFAKFLGMNISEVKYNYKLVKETSKAYNSIIALKDVTTIISNGESLFFNVTGNSSLSKGGSGDILSGLIAGFIAQNGDVLNGVLLGVYVFGLAAEMYEYEGHNLISEILDYIK",
    "response#3": "MRRADEIMQIEYGYLGVLLMETAGRRAAQLVRRLYPKQERFVVAAGGGNNGGDGLVIARYLRRYGKEIFILLTQDPAKLSALSRIQYDILTRFGLSCHVLSPDSLRKLQAFCEAEPRPVLIDALIGVGLQQALRPNQMALLEFLRSLHLPVVAVDLPSGLSADTGALLNEPLRCEHTITFQVPKICHLVTPAALYCGQVHVVDIGIYPEVLDDATLGALLPVRTASAHKGTAGRVWIVGGHPGTVGAPALAGLGALRAGAGLVTVHSAADVPLVTPELMAQRHADLRTFLTSTGLTGTGARPDAVAIGMGLGPEAPALAREVLDWEIPTVLDADALQPDLCGYGHDRCVWTPHPGEAARVLGVPTPEVTRDPLSAARALQDRLGGVVVLKGGPSVVARAGDLSVARGGHPGMASAGMGDTLCGILAALLAQGLDPWDAARAGVRLHARAGEIAAVRFGYGLGATDVAHDLGRAWADALLPVRTASAHKGTAGRVWIVGGHPGTVGAPALAGLGALRAGAGLVTVHSAADVPLVTPELMAQRHADLRTFLTSTGLTGTGARPDAVAIGMGLGPEAPALAREVLDWEIPTVLDADALQPDLCGYGHDRCVWTPHPGEAARVLGVPTPEVTRDPLSAARALQDRLGGVVVLKGGPSVVARAGDLSVARGGHPGMASAGMGDTLCGILAALLAQGLDPWDAARAGVRLHARAGEIAAVRFGYGLGATDVAHDLGRAALLPVRTASAHKGTAGRVWIVGGHPGTVGAPALAGLGALRAGAGLVTVHSAADVPLVTPELMAQRHADLRTFLTSTGLTGTGARPDAVAIGMGLGPEAPALAREVLDWEIPTVLDADALQPDLCGYGHDRCVWTPHPGEAARVLGVPTPEVTRDPLSAARALQDRLGGVVVLKGGPSVVARAGDLSVARGGHPGMASAGMGDTLCGILAALLAQGLDPWDAARAGVRLHARAGEIAAVRFGYGLGATDVAHDLGRA"
  },
  {
    "reference": "MTAQTAEEHSLTAVIGLEVHVQLETDTKIFCGCSTDADDNEEPNTRTCPVCLGLPGALPVLNEGAVEAAVKVGKALSSDIPEETRFHRKNYYYPDLPKNFQITQYDAPICDGGELEFAVDDERRTVAIERAHLEEDPGSLSHAGGSIDTAEHTLVDYNRAGTPLLEIVTAPDFRSAAEVRAFLAKLTEVLEYLGVFDVTRDGSLRVDANLSIVDSDAVGENGSIDEATLEAANRTEVKNISSHKGAEKALAYEETRQKNAVQRGREVEQETRHWDESRGITVSMRSKEEEKDYRYFREADIPPLRVSDWKEKIDIPELPDARRERFREEYGLDAETASKLTSTKQVADFYERVADTFAPELAATWVADNLLGELNYRDMEITDIEGRLDEFAHLIELVDEDEITVKNAEEVVLRRMLDDGQPPGDIVEAEDLGKTDDDAVAAAVTAAIEENPDAVEDYHAGEDGALNFLVGQVMQETGGSADPGTVNELLRAELDG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design must be able to perform ATP binding, glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity, asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity in order to contribute to translation.2. We are looking for a protein with broad substrate specificity for ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+)   + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate.3. The protein must exhibit the following characteristics: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl- tRNA or glutaminyl-tRNA synthetases; The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp- tRNA(Asn) or phospho-Glu-tRNA(Gln).. The designed protein sequence is ",
    "response#1": "TIGLEVHVELKTNSKIFSPSPVNFGAAPNANTNVIDWGYPGVLPSINKGVVRDGIMAALALHAEVTKKMHFDRKNYFYPDNPKAYQITQSETPIAHDGWVEIEVDGKKKKIGIKEMHIEEDAGKNTHTGSYSYVDLNRQGTPLIEIVSKADIASPEEAVAYLEALRQRIQFTGISDVKMEEGSMRVDTNISVRPYGSDQYGTKTEMKNINSFNFVKNALNFEADRHQKVLMAGGEIVQETRRYDEATKGTVAMRTKEGSDDYRYFPEPDLPPLEVDDAWIEELEAEAPELPTDKREHFFETATMSCKNYRALSNWITVEFAGRCKARGKTLPFTGILPEWVAQLVNFIDRGVITGKIAKEIADRMVSSFGESPEDILRRHPSLLPMTDDHALRAIVKEVVAQNTASVADYKNGKAKALGFLVGQIMKRTEGKAPPKRVNELLLAAL",
    "response#2": "VIGLEVHVELNTESKLFSPARNHFGDEPNTNISPVCTGMPGSLPVLNKDAVRKAVLFGCAVEGDVALFSRFDRKSYFYPDSPRNFQITQYEHPIVRGGCIRAVVEGEEKTFELAQTHLEDDAGMLKHFGDFAGVDYNRAGVPLIEIVSKPCMFSAEDAVAYANALVSILGYIGISDCNMEEGSIRFDVNISVRPRGSRELRNKVEIKNMNSFTFMAQALEAEKRRQIEEYLSYPNEDPKKVVPAATYRWDPEKKKTVLMRLKERAEDYMYFVEPDLPVLQITETYIDEVAKTLPELPEFYEVVAAAVEPRVAGTWVADVLKGELNYRDLSVDSFTTDDIIQIIKLVVEDKVTEQSAVDVIRTILDDGGTPMEVVTEKGLLKVKGDVVTEAVSETIAENEAAVQDYLGGAEKSLNFLVGQVMKKTKGRADARQARELLVAFFETATMSCKNYRALSNWITVEFAGRCKARGKTLPFTGILPEWVAQLVNFIDRGVITGKIAKEIADRMVSSFGESPEDILRRHPSLLPMTDDHALRAIVKEVVAQNTASVADYKNGKAKALGFLVGQIMKRTEGKAPPKRVNELLLELK",
    "response#3": "VIGLEVHVELNTKSKLFSSAQNRFGDEPNTNISPVCTGMPGSLPVLNKEAVNKAILFACAVQGEVALLSRFDRKSYFYPDSPRNFQITQFEHPIVRGGYVKAIVRGEERYFELAQAHIEDDAGMLKHFGEFAGVDYNRAGVPLIEIVSKPCMFCADDAVAYATALVSLLDYIGISDCNMEEGSVRFDVNVSVRPKGSEELRNKVEIKNMNSFAFMAQALEAERCRQIEAYLENPNKDPKTVIPGATYRWDPEKKKTVLMRLKERAEDYKYFIEPDLPVLQLTEAYIEKEKAELPELPRDKRDFYEVVAAAVEPRVAGTWVADVLKGELNYRDLSVDSFTTDDIIQIIKLVVEDKVTEQSAVDVIRTILDDGGTPMEVVTEKGLLKVKGDVVTEAVSETIAENEAAVQDYLGGAEKSLNFLVGQVMKKTKGRADARQARELLVAALSK"
  },
  {
    "reference": "MAMTVHVDIVSAEEQIFSGLAEFVALPGEAGELGILPGHMPLMTRIKPGAVRVKVQNQAEEEVVFVAGGLLEVQPGLVTVLADTAIRGKDLDEAKALEAKRKAEEALANQSSQLDYAKAQAELAEAIAQIAAIQRLKKGIH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that localizes to proton-transporting ATP synthase complex, catalytic core F(1), plasma membrane and can perform proton-transporting ATP synthase activity, rotational mechanism, ATP binding with high specificity and efficiency.2. The protein must exhibit the following characteristics: Produces ATP from ADP in the presence of a proton gradient across the membrane.. The designed protein sequence is ",
    "response#1": "IKVEIVSPEKVLFSRAVDMALIPGLEGDIAAMPDHAPMMLLLRGGVVELHQDGAVTDRFFVAGGFADMTETSCTILADQR",
    "response#2": "SIKVEIVSPEKVLFSRAVDMALIPGLEGDIAAMPDHAPMMLLLRGGVVELHQDGAVTDRFFVAGGFADMTETSCTILADQL",
    "response#3": "MGKLSLSLPHQAIYKSQDVVQVNIPAVSGEMGVLANHVPSIEQLKPGLVEVIEESGSNKQYFLSGGFAVVQE"
  },
  {
    "reference": "MTLLIMITLTSISLLLAAAETIPTTLDGPFKPLTRRFEPSLRRGSDDLPMDHPRLRKRNVSSDFPEQIALALSTPTSMWVSWVTGDAIVGKDVKPLDPSSIASEVWYGKEKGNYMLKKKGNATVYSQLYPSDGLLNYTSGIIHHVLIDGLEPETRYYYRCGDSSVPAMSEEISFETLPLPSKDAYPHRIAFVGDLGLTSNTTTTIDHLMENDPSLVIIVGDLTYANQYRTIGGKGVPCFSCSFPDAPIRETYQPRWDAWGRFMEPLTSKVPTMVIEGNHEIEPQASGITFKSYSERFAVPASESGSNSNLYYSFDAGGVHFVMLGAYVDYNNTGLQYAWLKEDLSKVDRAVTPWLVATMHPPWYNSYSSHYQEFECMRQEMEELLYQYRVDIVFAGHVHAYERMNRIYNYTLDPCGPVYITIGDGGNIEKVDVDFADDPGKCHSSYDLFFFNSLNLSN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Ensure the presence of a signal peptide within the protein.2. The presence of Mn(2+) is crucial for the protein to function optimally under specific conditions.3. The protein should be optimized for extracellular region-specific folding and stability, while maintaining acid phosphatase activity. The designed protein sequence is ",
    "response#1": "TFGIIGDLGQTYASNETLYHYMSNPKGQAVLFAGDLSYADDHPNHDQRKWDTWGRFMEPCAAYQPFIFAAGNHEIDFVPNIGEPHAFKPYTHRYPNAYKASQSTSPLWYSVRRASAHIIVLSSYSAYGKYTPQYIWLEQELKNVNREETPWLIVIVHSPWYNSNNYHYMEGESMRVMFESWLVNSKVDLVLSGHVHAPDVPYTFGIIGDLGQTYASNETLYHYMSNPKGQAVLFAGDLSYADDHPNHDQRKWDTWGRFMEPCAAYQPFIFAAGNHEIDFVPNIGEPHAFKPYTHRYPNAYKASQSTSPLWYSVRRASAHIIVLSSYSAYGKYTPQYIWLEQELKNVNREETPWLIVIVHSPWYNSNNYHYMEGESMRVMFESWLVNSKVDLVLSGHVHAYERSERISNIKYNITNGLSSPVKDPNAPIYITIGDGGNIEGIANSFVDPQPSYSAYREASFGHAVLEIMNRTHAQYTWHRNQDNEPVAADSIMLHGGCFPAEALVTLEGGGQKPISDLRPGDRVLSSSGSDGCGPLAFSEVLTFLDRDRTTPRLFHTLQTDSGARLSLTAAHLIFVSEGNCSEGAEPARGALRTAYAGDARPGQCVLVAY",
    "response#2": "GLERVTHIAHRSAFDYEVLLLKRSGNSGFMPNAYVFPGGLVDASDFSSEWQEVFQFFTKAPNYGIGVVKQPPETRPPIFATDRAQLGSPIPGDVAFRICAVRETFEESGVLLAVPKHEESGIRVRTEDARDSDPQPALTSLRGLWDRDVLSRWRPLVIGDSANFIKMCKELQCLPNIWALHEWGNWLTPIGTRRKQRRYDTAFYICFLRDAPHTLHDEKEIVHFKWSTPSEVLHSYESKEIWIAPPQFY",
    "response#3": "LTKDLGNFPANVCTPTYLANTAKKMAKEFKLEVEVLDRKQLQALKMGSFLSVTNGSVEPPKFIVLKHNGGKAKDAPTVLVGKGITFDSGGISLKPGAAMDEMKYDMGGAASVLGTMRAIAELKLKLNVIVVIPTCENMPSGSATKPGDIVTSMSGQTIEVLNTDAEGRLVLCDALTYVERFKPAAVVDVATLTGACIVALGHHNSGLFTRNDDAHDALANELLTAGKVAGDTAWRMPIEESYQEQLKSNFADMANIGGPAAGSVTAACFLERYTKKYTWAHLDIAGTAWKSGAAKGSTGRPVPLCFPAEALVTLEGGGQKPISDLRPGDRVLSSSGSDGCGPLAFSEVLTFLDRDRTTPRLFHTLQTDSGARLSLTAAHLIFK"
  },
  {
    "reference": "MGYRVAVIGATGNVGREMLQILAERNFPADEVVALASSRSTGQSVSFGENAVLKVQDLAKFDFKGIDIALSSPGARVSAEHSPRAAKAGAVVIDNTSYWRMDPDVPLVVPEVNREAIAGYKRRGIIANPNCSTIQMVVALKPLHDAATIKRVVVATYQSTSGAGKEAMDELLNQTKSFFVNQDLEPKKFAKNIAFNVIPQIDVFMEDGSTKEEWKMVVETKKILDPKIKVHATCVRVPTFIGHAEAVTIELEQPLEEDEARRLLAAAPGILVVDRREPGGYVTPKEVTGQDAVFVSRVRVDPTVEHGLSLWCVSDNLRKGAALNAIQIAEELIKGYI",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a high affinity for NADP(+).2. The protein design should take into account the physiological conditions of the L-threonine from L-aspartate: step 2/5 pathway.3. The protein design must be able to perform protein dimerization activity, NAD binding, aspartate-semialdehyde dehydrogenase activity in order to contribute to diaminopimelate biosynthetic process. The designed protein sequence is ",
    "response#1": "LAPLHRYSRIKRVVASSYQAVSGAGAKALEELKRQVRAWAAGEPLPVEVFPYQIAFNVLPHIDAFQENGYTNEEMKLVNETRKILEDDRILVSPTTVRVPVFTAHSVSVHVETEQKIPVATAREVFAAFPGLRVLDDPANKQYPMPLFVAGQDDCFVGRIREDLAVPNGLNFWVVGDQLRR",
    "response#2": "LAPLHRYSRIKRVVASSYQAVSGAGAKALEELKRQVRAWAAGEPLPVEVFPYQIAFNVLPHIDAFQENGYTNEEMKLVNETRKILEDDRILVSPTTVRVPVFTAHSVSVHVETEQKIPVATAREVFAAFPGLRVLDDPANKQYPMPLFVAGQDDCFVGRIREDLAVPNGLNFWVVGDQLRR",
    "response#3": "LKPLDNAVKIRRVVISTYQSVSGAGKLAMDELYKQTKAKFVFENLDARVFPKQIAFNLFPHIGEFKEDGYTDEEYKIQEELKKIIGNHLSTTVTCVRVPVFVSHSMSVNVEFEGKMDAVEAEELLYEADGIVVHSLENEMRYATPVDAVEHDDVFVSRIRNDHSKANCINMWICIDNLRG"
  },
  {
    "reference": "MKNILLAVTGGIAAYKAIDLTSKLTQANYDVRVMLTDHAQEFVTPLAFQAISRNPVYTNTFIEENPEDIQHIALGDWADCIVVAPATANTIAKLSVGIADDIVTSTLLATETPKFIAPAMNVHMYENKRTQHNISVLKDDEYHFIEPGDGFLACGYIAKGRMEEPLQIVKIINDYFKDKNELSHISYTSFEGKHALVTAGPTIEVIDPVRFVSNRSSGKMGYAIAEALAKRGAKVTLVAGPTQLDNPENVEVIHVESAEEMFNAVTSRYEEQDIVVKAAAVSDYTPADVLNHKMKKQDGNLSVTFKRTKDILKYLGEHKTHQYLVGFAAETQNINAYAQDKLKRKNADVIISNNVGDQTIGFQSDDNELTMHFKDGQRINIKKGKKVQLAQQILDELETRWQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein with the reaction H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2, the Proton donor must be optimized to ensure efficient catalysis.2. A protein that is capable of catalyzing reactions within the Cofactor biosynthesis, coenzyme A biosynthesis pathway with high efficiency and specificity.3. The designed protein must be able to perform FMN binding under specific conditions. The designed protein sequence is ",
    "response#1": "MRIVLGVGGGIAAYKSASLLRLFTEAGHSVQVIPTEASTRFVGIATWEALSGHPVSTSVFDAVDTVNHVRLGHEADLIVVAPATADLLAKAAAGLASDLLTNTLLMARGPVFFAPAMHTEMWNHPATRANVATLRSRGAIVMEPASGRLTGKDSGPGRLPDPEDIFAAFEALS",
    "response#2": "MRIVLGVGGGIAAYKSASLLRLFTEAGHSVQVIPTEASTRFVGIATWEALSGHPVSTSVFDAVDTVNHVRLGHEADLIVVAPATADLLAKAAAGLASDLLTNTLLMARGPVFFAPAMHTEMWNHPATRANVATLRSRGAIVMEPASGRLTGKDSGPGRLPDPEDIFAALEAALA",
    "response#3": "LGKSVLVTAGPTREDIDPVRFISNHSTGKMGMELARAASQRGASVTLILGDTKLKAPLHVNCIKVVSALEMFNAVKTEYKNYDIIIKAAAVADYRPKYPASEKIKKTDTDGTVHNNLNLELEKTDDILEFLGNNKTKNQFLCGFSMETQDVVENSRKKLYKKNLDMIAANSIKEKGAGFGVDTNIITLIR"
  },
  {
    "reference": "MADPAGTDGEGTGCNGWFYVEAVIDRQTGDNISEDENEDSSDTGEDMVDFIDNCNVYNNQAEAETAQALFHAQEAEEHAEAVQVLKRKYVGSPLSDISSCVDYNISPRLKAICIENNSKTAKRRLFELPDSGYGNTEVETQQMVQVEEQQTTLSCNGSDGTHSERENETPTRNILQVLKTSNGKAAMLGKFKELYGVSFMELIRPFQSNKSTCTDWCVAAFGVTGTVAEGFKTLLQPYCLYCHLQSLACSWGMVMLMLVRFKCAKNRITIEKLLEKLLCISTNCMLIQPPKLRSTAAALYWYRTGMSNISDVYGETPEWIERQTVLQHSFNDTTFDLSQMVQWAYDNDVMDDSEIAYKYAQLADSDSNACAFLKSNSQAKIVKDCGTMCRHYKRAEKRQMSMGQWIKSRCDKVSDEGDWRDIVKFLRYQQIEFVSFLSALKLFLKGVPKKNCILIHGAPNTGKSYFGMSLISFLQGCIISYANSKSHFWLQPLADAKIGMLDDATTPCWHYIDNYLRNALDGNPVSIDVKHKALMQLKCPPLLITSNINAGKDDRWPYLHSRLVVFTFPNPFPFDKNGNPVYELSDKNWKSFFSRTWCRLNLHEEEDKENDGDSFSTFKCVSGQNIRTL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The presence of SF3 helicase domains is critical for the interaction of the designed protein with its intended targets.2. The designed protein should contain at least one Nuclear localization signal motif to achieve its intended function.3. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.4. The ideal protein would be one that plays a critical role in DNA replication through DNA binding, DNA helicase activity, ATP binding within host cell nucleus.5. For general function, the protein need meet that Subsequently, two hexamers will be created; Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori; The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.; It forms a complex with the viral E2 protein; The E1- E2 complex binds to the replication origin which contains binding sites for both proteins; Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer; During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity; ATP-dependent DNA helicase required for initiation of viral DNA replication6. The protein should be designed to have high stability and activity towards ATP + H2O = ADP + H(+) + phosphate. The designed protein sequence is ",
    "response#1": "MADSKGSTSKEGLSDWCILEAECSDLENDFEQLFEQDTDSDVSDLLDNGELEQGNSLELFHQQECEQSEEQLQILKRKYLSPKAVAQLSPRLESISLSPQQKSKRRLFAEQDSGLELFKDTYGLSFTDLVRNFKSDKTTCTDWVTAIFGVNPTIAEGFKTLIQPFILYAHIQCLDCKWGVLILALLRYKCGKSRLTVAKGLSTLLHVPETCMLIQPPKLRSSVAALYWYRTGISNISEVMGDTPEWIQRLTIIQAEENEPNEQKFTLSTMVQWALDNDYTDECTIAYEYAKCADTDSNAAAWLNTNAQAKHLKDCATMVAHYRRGIMRSLSMSGWIHKRVQRAPDGGDWRHIIQFIKYQHIEVIVFLTALTHLLKGTPKRNCVLLCGPPNTGKSTFAMSLLSFMGGRVLSFANAKSHFWMQPIVDSRLVLIDDATKNTWDYIDEYLRNALDGNPICLDVKHRAPTQLKCPPILVTSNIDIREDDRWRYLHSRVCCFKFINEFPFNREGQPVYELDDKNWKCFFTRLWLQLDLSDQEDEADDGGAGDTFRL",
    "response#2": "MADNSGTEGEEEDCSEAERAGGWFMVEAIVDRRTGDTISSDEDEEDEGEDMVDFIDDRPIGDGQEVAQELLLQQAAADDDEAVHTVKRKFAPSPYFSPVCVPSIEHELSPRLDAIKLGRQSSKAKRRLFQLPDSGYGQQAGRFKEAYGVAYGELTRQFKSDKTCNGDWVVAVFGASPILYEGTNLRLQKHCESSLLTNTQTAAGAIALMLLRFKAQKSRDTLYKLLKGMLNVPEYQIMAEPPRIRSTPAAVYWFRLAMATSTQKHGELPEWVTRQTMLHHEQKFTLSTMVQWALDNDYTDECTIAYEYAKCADTDSNAAAWLNTNAQAKHLKDCATMVAHYRRGIMRSLSMSGWIHKRVQRAPDGGDWRHIIQFIKYQHIEVIVFLTALTHLLKGTPKRNCVLLCGPPNTGKSTFAMSLLSFMGGRVLSFANAKSHFWMQPIVDSRLVLIDDATKNTWDYIDEYLRNALDGNPICLDVKHRAPTQLKCPPILVTSNIDIREDDRWRYLHSRVCCFKFINEFPFNREGQPVYELDDKNWKCFFTRLWLQLDLSDQEDEADDGGAGDTFRKFKSSFGISFTDLTRVYNSDKTCSSDWVVCLYHVSDDRREAGKTLLQDHCEYFFLHSMGFCTLLLLCLFVPKCRNTLFKLCRSLFHISNVQMLADPPKTRSPAVALYWYKKGFASGTFTHGELPSWIAQQTLITHE",
    "response#3": "MADNSGTEGEEEDCSEAERAGGWFMVEAIVDRRTGDTISSDEDEEDEGEDMVDFIDDRPIGDGQEVAQELLLQQAAADDDEAVHTVKRKFAPSPYFSPVCVPSIEHELSPRLDAIKLGRQSSKAKRRLFQLPDSGYGQGEQKFTLSTMVQWALDNDYTDECTIAYEYAKCADTDSNAAAWLNTNAQAKHLKDCATMVAHYRRGIMRSLSMSGWIHKRVQRAPDGGDWRHIIQFIKYQHIEVIVFLTALTHLLKGTPKRNCVLLCGPPNTGKSTFAMSLLSFMGGRVLSFANAKSHFWMQPIVDSRLVLIDDATKNTWDYIDEYLRNALDGNPICLDVKHRAPTQLKCPPILVTSNIDIREDDRWRYLHSRVCCFKFINEFPFNREGQPVYELDDKNWKCFFTRLWLQLDLSDQEDEADDGGAGDTFRRFKEAYGVAYGELTRQFKSDKTCNGDWVVAVFGASPILYEGTNLRLQKHCESSLLTNTQTAAGAIALMLLRFKAQKSRDTLYKLLKGMLNVPEYQIMAEPPRIRSTPAAVYWFRLAMATSTQKHGELPEWVTRQTMLHHKFKGLADASFTELTRQFRSDRTCCADWVVAAFGITEILYESTKTVLAPHCLYMQLRHLAGTEGRVLLMLLQFKAAKNRCTVKKLLQGILGVPELVLLADPPRCRSVAVAVYWYRTGVSAGTYITGETPTWLKQQISISHRRSKLLEQKFTLSTMVQWALDNDYTDECTIAYEYAKCADTDSNAAAWLNTNAQAKHLKDCATMVAHYRRGIMRSLSMSGWIHKRVQRAPDGGDWRHIIQFIKYQHIEVIVFLTALTHLLKGTPKRNCVLLCGPPNTGKSTFAMSLLSFMGGRVLSFANAKSHFWMQPIVDSRLVLIDDATKNTWDYIDEYLRNALDGNPICLDVKHRAPTQLKCPPILVTSNIDIREDDRWRYLHSRVCCFKFINEFPFNREGQPVYELDDKNWKCFFTRLWLQLDLSDQEDEADDGGAGDTFR"
  },
  {
    "reference": "MNKSIFIQLQDQIDKEHSIREKLTAEVDLLDEKLRVLQLLLANCEQNLENQEEILEALEIIKSKTRGLAELASNFPYYKYNGVWDRSIQKVVYLYLLASWTGRLDKSLRPTYSLLSLSEVGQILQVPVFPEESTFHLSIEQYLHAVLSLCSELARQSVNSVISGNYHIPFEALNTIQKVHSSFQVLSLKNDSLRRHFDGLKYDLKRSEDVVYDLRIHKLV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Has much higher affinities for the homologous RNA sequences (GU)n and (GUU)n; Selectively binds single-stranded d(GT)n and d(GTT)n microsatellite repeats. The designed protein sequence is ",
    "response#1": "GVSSPISLAVPKETDCLLTQRLIETLRPFGVFEEEEELQRRILVLEKLNNLVKEWIREISESKSLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPSHVDRSDFFTSFYAKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLL",
    "response#2": "SAEQQAVIDCVLDQDLNVFYTGSAGTGKSALTRELIRQLRQKHGESAVAVTASTGLAAVNIGGTTLHKFAGIGIGVGDAKHLVGKVLSNNQTKQVWNQTKVLIIDEISMVDVTYFEKLDAVAQGVRKIRKPFGGIQIVLTGDFYQLPPVSQNTQKKFCFQSTVWSQLVQKTVLLTEVFRQQGDQRLIHMLV",
    "response#3": "GVSSPISLAVPKETDCLLTQRLIETLRPFGVFEEEEELQRRILVLEKLNNLVKEWIREISESKSLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPSHVDRSDFFTSFYAKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLS"
  },
  {
    "reference": "MRVLVQRVTSAQVTVDGEVVGRIEPNPQGLVALVGVTHGDDDATARALADKLWRLRILDGERSAADLGAPILVVSQFTLYADTAKGRRPSWSAAAPGPVAEPLVDAFAATLRELGATVATGRFGAHMHVELTNDGPVTLLLES",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should contain at least one Gly-cisPro motif, important for rejection of L-amino acids motif to achieve its intended function.2. The designed protein should have D-aminoacyl-tRNA deacylase activity, Gly-tRNA(Ala) hydrolase activity, Ser(Gly)-tRNA(Ala) hydrolase activity, tRNA binding within cytoplasm to regulate D-amino acid catabolic process.3. The protein should have a fast turnover rate for a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + a tRNA +   H(+).4. The protein must exhibit the following characteristics: Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS; An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs; Acts via tRNA- based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site; By recycling D- aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl- tRNA entities in vivo and helps enforce protein L-homochirality.. The designed protein sequence is ",
    "response#1": "MTTAPLAGRDEDGGPVLKXGAXRXGXGXDLXGXIXGXFXGXDXKXGAXALXAXXXGXGXVXPXGXAXAGXTXPGXAXEGXDXNVXGAGXIV",
    "response#2": "MAMDMPIKQNLPDRGSSSRLVPLTXMAXVXGXPXGXFXVXQXVXDXNQXFXK",
    "response#3": "MPGPTTVXGPGGAXPXGVXPXGVILXGXVGXAXGXGXAXXRXAXPTXEXTXXXLPXVXVXXGXLAXGXLXTGXDLXXFXHXSCX"
  },
  {
    "reference": "MPEAFERYSSNPTYEPPWRKVRFIGIAGPSGSGKTSVAQLIVKALNLPHVVILSLDSFYKSLNAEQKKRAFNNDYDFDSPEAIDWDLLFVKLLELKQGRKVDIPIYSFNEHNRLPETNTLFGASIIILEGIFALYDEKIRSLLDVSVFLDTDSDVCLSRRLNRDINYRGRDIVGVLEQYNKFVKPSYENFVRRQLSYTDLIVPRGRDNKLAIDMVINFIRRTLSIQSETHVKNIDSLQQIVPTIPHLPLNLVQLKITPEISAIRTILINKNTHPDDLQFFLSRIGTMLMNLAGDSLAYEKKTITLHNGNQWEGLQMAKELCGVSVLRSGGTLETALCRQFPTVCLGKILVQINKVTQEPTLHYHKLPRGIATMNVVLMASHLTTHADVLMATQILVDFGVPEENIIIVVYVCYSESIKALAYIFPKVTIVTAFLESVAEPVVGRLDIEKVYYGC",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should consider the endogenous proteins and enzymes present in the UMP biosynthesis via salvage pathway, UMP from uridine: step 1/1 pathway to avoid interference with native metabolic processes.2. The designed protein should be involved in phosphorylation, pyrimidine nucleobase metabolic process by localizing to nucleus, mitotic spindle pole body and exhibiting uracil phosphoribosyltransferase activity, uridine kinase activity.3. The protein should have a fast turnover rate for ATP + cytidine = ADP + CMP + H(+). The designed protein sequence is ",
    "response#1": "VMDHPLIQHKISLLRDKNTQTKEFRELVSEISMLMGYEVTRNMPLKEVEIETPVGVARTKVISGKKMGIVPILRAGLGMVDGMLKLLPMAKVGHIGLYRDPETLQPVEYYCKLPCDIAEREIVVLDPMLATGGSASAAIKYLKERGVTSIKLMCLIASLDGIKKINDEHPDVLIYCAAVDDKLNEHGYIIPGLGDAGDRLFGS",
    "response#2": "LDHPLIQHKLSHMRDRDTSTRTFRELLREITLLMGYEITRNLPMTTRRVSTPLVDIDAPVIAGKKLAIVPVLRAGVGMSDGLLELIPSARVGHIGVYRDDDHRPVEYLVRLPDLEDRIFILCDPMVATGYSAVHAIDVLKRRGVAGENISFLALVAAPEGVQVFQDAHPDVKLYVASLDSHLNEHAYIIPGLGDAGDRLFGL",
    "response#3": "RHPLIRHKLGLMRRADISTKNFRELAQEVGALLTYEATADLTLENYDIQGWAGTVSVEKIAGKKITVVPILRAGIGMLDGVLSLIPGAKVSAVGVARNEETLQAHTYLEKLVPEIDERLAMIIDPMLATGSSMVATIDLLKKAGCKEIRAMVLVAAPEGIAAVEKAHPDVMIYTASIDERLNEHGYIIPGLGDAGDKIFGR"
  },
  {
    "reference": "MATTVVILAAGQGKRMRSDLPKVLHPLAGRPLLEHVLVAAGALRPREIRVVHGHGGERVKDAFAGRELTWVHQAEQRGTGHAVRQALPRIPPKDVVLILYGDVPLVRPATLRRLVAKARTGQLAVLTAAFDDPAGYGRIIRSGGSRGGIVAIVEDKDATPAQRAIREINTGLLACPAGALARMVRRLRADNAQKEYYLTDIVALAVRDGMGVAAVAAASADEVMGVNDKAQLAEAGRLLRRRIAGELLQAGVTLVDPERIDVHGSLRCGRDVTIYPDVLFEGEVSLGDGVVVEPFSRIRNTKLGAGTVVHSHCVIESGDIGPGCEIGPYARMRPGVVLRGHVKLGNFVEVKKSTVGLHSKVNHLTYIGDTTIGERVNVGAGTITCNYDGANKHHTVIGDQAFIGSGVELVAPVTIGAGATIGAGSTISKDAPPGELTVARSKQLTIPGWKRPVKQPK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The desired protein should be able to perform acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate2. The protein design should aim to mimic or enhance the function of natural proteins involved in cell morphogenesis, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "LPKVLHPICGRPMLAYVLDAAREATGQRPLVVYSPATAQIRDVFADEADFALQDQPRGTGDAVRVALSSLDDGATNLLVLNGDKPLLVAAPLRSLMQRQMDATAAMTLTTFVVSSNSPWGRVDFGPDGQAERIVERKDDPGRAELSAFHANAGIYAFDLAWLRGAVPRVEPSSASGELYLTQLVEIACKDGCPVATIDDALGR",
    "response#2": "LPKVLHPICGRPMLAYVLDAAREATGQRPLVVYSPATAQIRDVFADEADFALQDQPRGTGDAVRVALSSLDDGATNLLVLNGDKPLLVAAPLRSLMQRQMDATAAMTLTTFVVSSNSPWGRVDFGPDGQAERIVERKDDPGRAELSAFHANAGIYAFDLAWLRGAVPRVEPSSASGELYLTQLVEIACKDGCPVATIDTVDSLREKLIGG",
    "response#3": "ALILAAGKGTRMKSKLCKVLHTAAGRSLIDYVLDSVQALGAAKIVLVVGHQSQEVITAIELAGVEYAIQEPQLGTGHAVMAARDLLQGSTGHILILCGDIPLIRSETLSRFVECHAANNSGITVMTTIMDDPLGYGRIIRDDYGLIRAIVEEKDATPDQRLVCEINSGIYLVRAELLFSLLDRIGSDNAQHEYYLTDIVREAVCDGMPVHGFLVEDAMEISQFTYFQQVGGLECSPVSYEVSYGIERLAMYLQGVDSPHTIQWGSQIGKNVTVGPFAHLRPKTVLHEGVHVGNFVEIKDHEIREALEQGATLIDPESVWFSADTQLGRDCTIEPHVVFGPGVKIADGAVIRAFSHIEGATIATGCEVGPFARLRPGAVMERGSKVGNFVEMKKTVLGEGAKANHLTYLGDSEVGAKANIGAGTITCNYDGFFKYPTKIGAGAFIGSNSALVAPVTIGAGAIVGAGSVVTKDVEADELAVARGECRYSLEKEALEQGATLIDPESVWFSADTQLGRDCTIEPHVVFGPGVKIADGAVIRAFSHIEGATIATGCEVGPFARLRPGAVMERGSKVGNFVEMKKTVLGEGAKANHLTYLGDSEVGAKANIGAGTITCNYDGFFKYPTKIGAGAFIGSNSALVAPVTIGAGAIVGAGSVVTKDVEADELAVARGECSYQPERT"
  },
  {
    "reference": "MRIEEDIKLGFKDVLIRPKRSILKSRSQVNLARCFSFKYSASIWSGIPIIAANMDTIGTFEMVKSLSKFNILTAVHKYYSFEEWKNFVCLSSKEILNHVIVSIGTSNIDFLKIKKIFLLSSELKYICIDVANGYSEHIVSFLKLVRDYFPDKIICAGNVVTGEMVEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAIIECADAAHGLNGQIISDGGCTVSGDIAKAFGGGADFVMLGGMLSGHKECSGDIIEEKSKKYMIFYGMSSVSAMQRYEGKIAGYRASEGKTVKIPFRGGVDSTIRDILGGLRSSCTYVGAEKLKELTKRTTFIRVTEQENCIFNAFKE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for K(+) binding.2. The protein must exhibit the following characteristics: It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides..3. The protein should have a fast turnover rate for IMP + NADP(+) + NH4(+) = GMP + 2 H(+) + NADPH.4. The designed protein should have a specific function within the GMP reductase complex. The designed protein sequence is ",
    "response#1": "LDFKDVLLRPKRSTLKSRADVDLVREFVFRNSKKKYVGIPIVASNMDTVGTFEVAESLSKKRLFTTIHKHYSVDQWMEFVNRISSNQDILSQIGISSGISDYDFTKLKKICGLIPELQYICLDVANGYSEVFVDFIRRVREEFPRHTIFAGNVVTGEMTEELILSGADVVKVGIGSGSVCTTRKKAGVGYPQLSAVLECADASHGLNGHVMSDGGCTNPGDVAKALGAGADFVMIGGLFAGHDQCGGDTVEKDGQKYKLFYGMSSDTAMEKHEGSVAEYRASEGKTITVPYRGDISKTVQDLLGGLRSACTYTGAKKLKELSKRATFVRVTQQD",
    "response#2": "LDFKDVLLRPKRSTLKSRADVDLVREFVFRNSKKKYVGIPIVASNMDTVGTFEVAESLSKKRLFTTIHKHYSVDQWMEFVNRISSNQDILSQIGISSGISDYDFTKLKKICGLIPELQYICLDVANGYSEVFVDFIRRVREEFPRHTIFAGNVVTGEMTEELILSGADVVKVGIGSGSVCTTRKKAGVGYPQLSAVLECADASHGLNGHVMSDGGCTNPGDVAKALGAGADFVMIGGLFAGHDQCGGDTVEKDGQKYKLFYGMSSDTAMEKHEGSVAEYRASEGKTITVPYRGDISKTVQDLLGGLRSACTYTGAKKLKELSKRATFVRK",
    "response#3": "LGFKDVLIRPKRSILKSRSQVDLTRLFIFKHAQNKWSGIPLIAANMDTVGTFKMAIALSSFGILTAIHKYYSYLDWKKFIHTIPGTVVKHIMVSTGMLDEDFVKLKQILSLSSKLKYICIDVANGYSEKFVTFLKKVRECYCDKIICAGNVVTGEMVEELILSGADIVKVGIGPGSVCTTRAKTAIGYPQLSAVIECSDAAHGLGGQIISDGGCVVSGDIAKAFGGGADFVMLGGMLAGHKECEGLIFKENKKRYMIFYGMSSKFAMDRHIGGVARYKTPEGKTVKVLFRGAVSETINNILGGLRSTCTYVGAFTLKELTKRTTFIKVSP"
  },
  {
    "reference": "MTVRTRIAPSPTGDPHVGTAYVALFNLCFARQHGGQFILRIEDTDQARSTAESEQDILTALRWLGLNWDEGPDVGGPHGPYRQSERKHMYGQYAEDLVTAGHAFYCFRTPEELDAIREERKAQGLNPGIKGDLELPEEEVKRRLDAGDPYVIRMKVPDEGVCEIQDMLRGTIEIDWAQVDCQILLKSDGMPTYHLANVVDDHLMGITHVLRGEEWINSAPKHKLLYQYFGWDMPELCHLPLLRNPDKSKLSKRKNPTSINFYERMGFLPEAVTNYLGRMGWSMPDEREKFTLDEMIENFDIQRVSLGGPVFDVEKLRWLNGQWIREELSDEQFMARMQQWWFNQDDLKALVPHIKGRAEVFSDVAPMAQFMFSGMLNLKPEDFAHNKLDESQVKRVLQFALWKLEAQRHWSKNTIFADIKALAKAMDLKMGDFMFSIFVAIAGTPNSWSVMDSMALLGPDMTRSRLRHALEVMGGFSKKETKKVEKEYAALGV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)..2. The designed protein should contain at least one 'KMSKS' region motif to achieve its intended function.3. The protein should have a specific function in glutamyl-tRNA aminoacylation and should be localized to cytoplasm in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "LAEKARPHVEAAGYDVSDDRLQTICGLVQDRIQVVPEVVTDNRYFFEDPEAYEEAGVEKRWNEESADLLLAYADRLEGVDAFDTDTVETELRDLADEKDVGAGAIIHPSRLAVSGRSYGPGVFGLLAAVGKEACIRRMHRG",
    "response#2": "VVEAVRPFMQEAGYLSATPNPAELDYLSRVVEAVRSRVHTLAELRDASAYFYRSDFEYDDKGVRKHFTKPGVTNILARGRDALSRLPAGRFTVEGTEEAFRQVIEELGVSGGTLIHPTRLALSGRTVGPGLFDIIAVLGKEECLLRLDRAA",
    "response#3": "MRLFMVKNAKKLKIKNLQSTIVEVPNHPINDLGKRKIEITEDFYISGDDSEAIKEQSTIRLLGLGNVKITKIGNELEV"
  },
  {
    "reference": "MSGTSLAKIRNIGITAHIDAGKTTTTERILYYTGVSHKIGEVHDGNTTTDYMEQERERGITITSAAVTCEWKDHRINIIDTPGHIDFNIEVNRSLRVLDGAVFIIEGVAGVQPQSETNWRLADRYNVPRIIFINKLDRTGADFYRAFDTLKEKLDIVALPLQLPIGIEDQFVGVVDLVEMKAIVWEGGELGAKFHDEEIPADMLEKAKEYRQNLLDTALSVDDAGMEEYFEKGDVATETLKRAIKTGTISGAFRPVLCGTAFKNKGVQPLLDAVLDYLPSPVDIEGIKVAPPEGEEEDPSANRRVIPANPDAPFAGLAFKIINDKYGTLTFVRVYAGTLRSGDTVLNTTKGHKERVGRMFQMHADKREEVKEVHAGDIAAFVGLKDTGTGDTLASSDDPVVLERMAFPVPVIDISVEPKTKEAVEKMTLALQKLAGEDPSLRLKTDQETGQTILSGMGELHLDIIIDRLRREYGVEANVGAPQVAYRETITREHTETYTHKKQSGGSGQFAEVKIIFEPQERNEGILFENKVVGGAVPKEYIPAVEKGIKVQADTGVLAGFPTVDFKYTLVDGKYHDVDSSALAFEIAAKACFREGMKKAGPVILEPIMDVEITTPQDHVGDVVGDLNRRRGMIQNQESSGSTVIVRAQVPLKEMFGYISNLRSMTKGRASFTMQFHHYDPVPRNIADEIMTKSA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.2. The protein should have guanosine tetraphosphate binding, GTPase activity and be active in cytoplasm.3. The protein should be able to bind GTP ligand in a variety of conditions4. The designed protein must have at least one tr-type G domain. The designed protein sequence is ",
    "response#1": "KPHVNIGTIGHVDHGKTTLTAAITAVLAVTNGAKMMDYDAIDNAPEERERGITIATSHVEYETNNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSKQVGVPYIVVFMNKEDMVDDEELLELVEMEIRELLDMYDFPGDDTPIVAGSAKEALDEAKTGTLGPWSAKIQKLMAAVDEYIS",
    "response#2": "NFSIIAHIDHGKSTLSDRLIQVCGGLTDREMAEQVLDSMDLERERGITIKAQSVTLDYTAKNGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALEMDLEVVPVLNKIDLPQAEPERVAAEIEDIVGIEATDAVRCSAKTGVGIGEVLETI",
    "response#3": "NFSIIAHIDHGKSTLSDRLIQVCGGLTDREMAEQVLDSMDLERERGITIKAQSVTLDYTAKNGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALEMDLEVVPVLNKIDLPQAEPERVAAEIEDIVGIEATDAVRCSAKTGVGIGEVLETV"
  },
  {
    "reference": "MEYLPIFVDIKQQTCLVAGGGPIALRKLENLLRAGAMVRIVSPTLVPELAARIDAPNIIWHQRRFQDADLQGARLVIAATDDRGVNRQIADLAKAAGIPVNVADQPEDCSFIMPSIIDRSPVVVAVSTGSASPVLARMIRARLESLVPAGYGRLAELCARYRNRVKERFDQQRDRRRFWDRVLQGGVAERIFSGHDDEADAAMERELAAGGSPSDVGEVYLVGAGPGDPDLLTFRALRLMQQADVVVYDRLVAKPIIDMVRRDAEHIYVGKQRNQHTMRQEQINALLARLAKQGHRVLRLKGGDPFIFGRGGEEIDTLAAEGVPFQVVPGITAANGCACYGGIPLTHRDYAQSVTFVTGHLKDGSMNLNWEQLAQPQQTLVFYMGLVGLPIIISNLIEHGVPADMPIALVQQGTTHMQKVYAGTLRSILGQVQADPPAPPTLVIVGKVVQLRDKLAWFSTIPDPNQGATTPLLPGD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.2. The protein must exhibit the following characteristics: Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.; Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin.3. The protein should be optimized for activity in sirohydrochlorin from precorrin-2: step 1/1, siroheme biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "VGAGPGDPDLLTFRALRLMQKADVVVYDNLVSKPIVEMARRDAERIYVGKKRDDHTLRQEEINELLVRLAKEGKRVLRLKGGDPFIFGRGGEEIETLAAEGIPFQVVPGITAASGVASYAGIPLTHRDHAQSCMFVTGHLKDGTMNLDWGALARPRQTVVVYMGLHGLETLCSELIKHGMPDTTPIAIVQQGTTQNQRVITGTLATLPAIAEREKPQAPTLIIVGGVVGAGPGDPGLLTLRALRALQNADVVLYDRLVSTQIVDLARRDAERIYVGKAPGDAHASQEQINALLVKLARQGKRVCRLKGGDPFIFGRGGEELETLAAEGIRFEVVPGITAAAGCAAYAGIPLTHRDYAQSLTFVTGHCKGETDKVDWDLLARPGQTVVFYMGLGHLESIVARLRERGVPAERAAAIIEHGTQATQRVVTGTLADLVHKAGEARIESPALLIVGEL",
    "response#2": "GEVYLVGGGPGDPDLLTFRALRLMQQCDVCVYDKLVSKEVMELVRRDAELINVGKARDQHTLPQEEINLLLVALAKEGKRVLRLKGGDPFIFGRGGEEIETLMENGVPFQVVPGITAANGVSSYAGIPLTHRDYAQSCVFTTGHLKSKDGQSHTVELDWPALARPNQTVVIYMGLIGLEQICIQLIKHGLDATTPAAIVQQGTTPKQKVVVGDLSDLAAKAALAELKPPCLIIVGHVVKLHG",
    "response#3": "GEVYLVGAGPGDPDLLTFRAMRLMQQADVVIYDRLVSPAILDMVRQDATRIYVGKVRNQHTLPQTSINELLVKLAQEGKHVLRLKGGDPFIFGRGGEEIETLSQHHIPFQVVPGITAASGVASYAGIPLTHRDHAQSCVFVTGHLKDNTIQLDWPALARPNQTIVVYMGLLGVTELCRQLIAHGLQATTPAAIVQQGTTPNQRVLTGTLETLPDIIQQNPLKPPTLIIVGNVVKLGEVSLVGAGPGDAGLLTLRGLQVIQQADVVLYDYLVSADVLELVRRDAEMICVGKRASAHLMPQDEINRRLVTLAQQGKRVVRLKGGDPFIFGRGGEELQHVAKAGIPFQVVPGVTAAAGVTAYAGIPLTHRDYAQSVAFITGHHRVDGKAPDWSTLARERQTLVIYMGTMKAADISRQLIENGRSAATPVAVISRGTCRDQQVLTGTLQQLEPLAQQAATPALLVIGEVVNLYG"
  },
  {
    "reference": "MILPILGGPTGSGKTSLTQVLDPKRFEIVSFDSRQVYRDLPVGTTAPTPEECSTIRHWLIGFLNANESINANQFSLLARNAIADIQSRGKIPFLLGGTGFYLRAFLLGMYPVPTVPKETKDYVFTLPLEEARSQLLAKDPKAMESLSDQDGYRIKRALEVVLTGVLWSDVSKETVGGFWKDNPEVKIVGHWLDWPREILYQRINTRVETIIRGMLEETKEVLSKYGPDCPGLRTLGYNFALAFLNGMIDSNTFIEQLAQSHRNYAKRQITWFKKESFLSPISYDAAVQLYTNIEQR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a high affinity for substrate, ATP.2. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.3. The protein should be designed to have high stability and activity towards adenosine(37) in tRNA + dimethylallyl diphosphate =   diphosphate + N(6)-dimethylallyladenosine(37) in tRNA.4. The protein must exhibit the following characteristics: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)..5. A protein with tRNA dimethylallyltransferase activity functions is required to enable tRNA processing. The designed protein sequence is ",
    "response#1": "MNDPNNQPTINDAQSTTHFGYQQVPVAEKAARVREVFDSVAGRXDLMXRXFXXXLXKKXXXVTLXXLPXXSXLDLAGGTGDXAFGFARRVGNKGQVWXSDINASMLKVGRDRLIDKGVLGNVTLACGDAEYLPFPDNSFDCVTIGFGLRNVTDKDAALRSMYXALKPGGRLLVLEFSKVATPGLAKXYDTYSFQVLPKLGQMVANDADSYRYLAESIRMHPDQETLKXMMSEAGFVNTHYHNMTGGIVALHSGYKF",
    "response#2": "MDYILSAREEIKRGIEEGKFGIIPMMQGKIDYETLKKDEKIRKSCEAVRIGLEYGADGVMLFPLRKNEDTKKEQIDFIENELKEYDLPLMIEGYESIPDSVSLVITAEDRERALKSAEAAKEAGADFIKAPGGESFPTAKDVAKVREAIPEEVYIKASGGIRTKKDVLEYIENGADIVGTSSGIKIAEEFLALAEK",
    "response#3": "MANTVLATHNAHKLKEMRSILAPLGIDVRSAAELDLPEPEETGTTFAENALIKAHAAAAHTGLPALADDSGICVDVLGGAPGVYTADWAETEDGRDFVMAMNRTWDLLAETEAPTGRFATTMCLAWPDGHTVVAEGRVDGRLTWPPRGEHGHGYDPMFQPDGYDITFAEMDAATKHQFSHRAQAFAKLVAEQFG"
  },
  {
    "reference": "MEEADKRAEELRAQIAECEATLQSLKEQLAAAEAAKTPPYSDSTETDRGSSSSTWKWPLAEAEYERYGRQLILPSVGIQGQLRLKAASVLIVGAGGLGCPASAYFAGAGVGTIGLVDGDTVEASNLHRQVAHGTSRVGMLKVDSAISYLRELNPLVKYNAHQSHLTPENAESIVSGYDLVLDCTDHPTSRYLISDVCVLLRKPLVSASALRTDGQLIVLNTPAAPQADLSGGPCYRCVFPKPPPPDAVTSCGEGGILGPVVGVMGVLQALEGIRLLAAGRHLSPSPEQQQTAISPSLLLFSAPPDGSPAGFRSVRMRGRRKDCFACGEKSALSLATLREGGLDYVQFCGGSRKPVALLKSEERVSAEQLNALLQQQAGEHGKPVLLDVREREHFEIANIPGAINIPFSKTQNPGARHNAEDTPKLDWLPDGVADGHSPVYVVCRVGNDSQTVARQLKEFGLDNQGKRFIGDVKGGMLAWKREVDSTLPFM",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A key design requirement for the protein is the optimization of the Glycyl thioester intermediate; for adenylyltransferase activity, Cysteine persulfide intermediate; for sulfurtransferase activity to achieve high catalytic efficiency for [molybdopterin-synthase sulfur-carrier protein]-C-terminal   Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] =   [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-NH-   CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase].2. Target a Polar residues compositional bias in the protein's composition for improved properties.3. The protein must exhibit the following characteristics: Its N-terminus first activates urm1 and MOCS2A as acyl- adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (- COSH) of their C-terminus; Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A; Also essential during biosynthesis of the molybdenum cofactor. The designed protein sequence is ",
    "response#1": "CHSYDIIVDATDNAPSRYMINDCCVVLGKPLVSGAAVGLEGQLTVFNYNGGPCYRCLFPTPPPTTACQRCSDAGVLGVVPGVIGCLQALEAIKIASAVGEPLSERMLLFDALSGRIRNVKIRGRSPQCCHSYDIIVDATDNAPSRYMINDCCVVLGKPLVSGAAVGLEGQLTVFNYNGGPCYRCLFPTPPPTTACQRCSDAGVLGVVPGVIGCLQALEAIKIASAVGEPLSERMLLFDALSGRIRNVKIRGRSPQCLRILYQDICVLLSKPLVSASAFQTSGQLIVLNNPPGKGPCYRCVFPTPPPPDSVVGCGEGGIIGPIVGTMGVLQALEAIKLISRGDLEIHGEAKTPMLLLFSGMSDTPFRSVRMRGRRKTCGVSRLNPEIQTNIYQTHLTPQNAEDIVQKYDLILDCTDHPTSRYLISDICVLLQKPLISASALRTDGQLIVLNHPPLPVGNISGGPCYRCVFPKPPPPESVVSCGDGGIIGPVVGTMGVLQALEAIKFIASGKLEVVHQDEGKTNPTSMLLFSTNSTPPFRNVRLRARRPACDQTITYSQFKAASGEAHVLVDVRPEHHFKIVSLPNSLNIPLPRLEARLPEITSALKEKEDQRGTDSGSGGQVYVICRRGNDSQRAVQYLQKMGFTSAKDIIGGLEGWAHEVDPNVCQ",
    "response#2": "MYSRQLILQDFGVQGQLKLKNSSVLIVGMGGLGCPAAQYLVAAGCGHLGLIDYDEVERSNLHRQILHSEHRCGMSKAESARIALLELNSHCQIRCHSRLINSMNAMHIIRPYDVVLDCSDNVATRYLLNDACVMLRKPLVSGSALKMDGQLTVYGYGQGPCYRCIYPVPPPPEAVTNCGDGGVLGAVTGIIGAMQALEAIKVIIGLGDVMSGRLLIFDGSSFMFRNIRIRTKRPNCHYARQLIIPQVGVHGQLRLKKASVLIVGAGGLGCPAAAYLAGAGIGTIGLTDGDIVEVSNLHRQIAHSTERVGILNPTIGYRAHHEHLTPQTAGSIVSQYDLVLDCTDHPTSRYLISDACVLYQKPLVFASALQTHGQLMVMNCPARPQGDRGGGPCYRCVFPKPSPPETVVSCGEGGILGPVVGVMGVLQALEGIKILAAGKHEQSYMTSPDENPIQHHMLLFSGHVATPFRSVRMAGRRKTYGRQLIMPEIGLQGKLRNARVLIVGVGGLGCPAAAYLVGAGVGTIGLMDGDTVEESNLHRQILHSTARVGMSKVESAMVNLSSLNPNVKLIPHKSRLAPETAIATFQNYDLILDCTDTPASRYLISDTCVLLCKTLVSASALRIDGQLMVLNNPPLPPGDPKGGPCYRCIFPKPPPPESVLSCGEGGILGPVVGVMGVLQALEAIKVLTQKKPAAGSDISPDIPSLLIFSAYSTPMFRSIRLRSRKPKCYGRQLILPNVGIQGQQRLKASKILIVGAGGLGCPAAAYIAGAGVGTLGIVDGDVVEPSNLHRQVAHSTFRVGMLKVDSLVAYCKGLNPLPEYIPHREHLTPQNAESIVGAYDLVLDCTDHPTSRYLISDACVLLRKPLVSASALRTDGQLIVLNCPPTPQGVVVVSPQPPSSAAGKPAAAAAAAASTPALPPCYRCVFPKPPPPESVVSCGEGGILGPVVGVMGVLQALEAIKIVAAGFHLPAVTAAAAAAPTPPPPTLLLFSGSAVGAPSFRSVRMRARRKD",
    "response#3": "CHSYDIIVDATDNAPSRYMINDCCVVLGKPLVSGAAVGLEGQLTVFNYNGGPCYRCLFPTPPPTTACQRCSDAGVLGVVPGVIGCLQALEAIKIASAVGEPLSERMLLFDALSGRIRNVKIRGRSPQCQRCKKAAGGAISPPSSPRTMSEAEKDEYLRKLKAEKIKAREEEARKQEEERKRKEKEKAAKEEEAKKAAEKEEHLLLDVREKEHFDLCNIAGSVNIPISRFMNARGETIPEGWPTDLPPSAPIYIVCRVGNDSQIAAKKLKDLGLGNNGERFIGDIQGGMRSWKYGRQLIMPEIGLQGKLRNARVLIVGVGGLGCPAAAYLVGAGVGTIGLMDGDTVEESNLHRQILHSTARVGMSKVESAMVNLSSLNPNVKLIPHKSRLAPETAIATFQNYDLILDCTDTPASRYLISDTCVLLCKTLVSASALRIDGQLMVLNNPPLPPGDPKGGPCYRCIFPKPPPPESVLSCGEGGILGPVVGVMGVLQALEAIKVLTQKKPAAGSDISPDIPSLLIFSAYSTPMFRSIRLRSRKPKCLRILYQDICVLLSKPLVSASAFQTSGQLIVLNNPPGKGPCYRCVFPTPPPPDSVVGCGEGGIIGPIVGTMGVLQALEAIKLISRGDLEIHGEAKTPMLLLFSGMSDTPFRSVRMRGRRKTCSRLNPEIQTNIYQTHLTPQNAEDIVQKYDLILDCTDHPTSRYLISDICVLLQKPLISASALRTDGQLIVLNHPPLPVGNISGGPCYRCVFPKPPPPESVVSCGDGGIIGPVVGTMGVLQALEAIKFIASGKLEVVHQDEGKTNPTSMLLFSTNSTPPFRNVRLRARRPA"
  },
  {
    "reference": "MGAIRRIVYAQSGGMTAVINASAAGVIAEAQRRGLTLFAARYGMHGLLAGDLIAVPPLSAEQLQRLSYTPGGIFGTARRDLPDPEIDATPYDLLFQVLAHYDIDALLYNGGNGSIATVAQIDREAKRRGIPLTVIGIPKTIDNDLEGTHFSPGYPSAARYLITSFLEASLDLASFPAKGARAFVMETMGRNAGWLAAAVKAASAVLADEGPHLIVLPEMRWDKTALLAAADAAIARTGYCTVVVAEGATLDGEPLAYQGAEKRRYVQLGGAGSVIGGWLRDALGAKVHVAVPDYLQRAAAHWVSAVDHRVAWQVGETAVAWADEGTRSGVMVGVVRDPATSGWRMEAVDAATVGNRERRFPASWIAAPFQVDSAFLDYLLPLLEGCPQIPCGAHGLPDFFPFATLTERSDAP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that localizes to cytoplasm and can perform 6-phosphofructokinase activity, metal ion binding, diphosphate-fructose-6-phosphate 1-phosphotransferase activity with high specificity and efficiency.2. The protein must bind to Mg(2+) in order to perform its enzymatic function.3. The protein design should take into account the physiological conditions of the D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 3/4, glycolysis, Carbohydrate degradation pathway.4. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.5. The substrate, Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand.6. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis; Consistently, PPi-PFK can replace the enzymes of both the forward (ATP- PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.. The designed protein sequence is ",
    "response#1": "LLVGQSGGPTAVINGSLYGVVSEGLKKTDEIGHVYGMVNGIEGFLNDNYMDMKSLEETNELELLRTTPGAFLGSCRYKLPEELDNEVYPTLFQKFEALDIGYFFYIGGNDSMDTVSKLSRYAEKVDSSIRFIGVPKTIDNDLVETDHTPGFGSAAKYVATTVREIATDASVYDNKKSVTIVEIMGRHAGWLTAASVLARKFEGDNPVLIYLPEVAFDQDAFVAKVEEALKTTPNLVVCISEGINDGKGTFICEFASEVGTDNFGHKMLTGSGKYLENLVKAKLGVKVLGGNFERRFIPKIGVVLSGGQAPGGHNVISGIFDYLQDHCKGSTLYGFRGGPAGIMKCKYVVLTPEYIYPYRNQGGFDMICSGRDKIETPEQFKQAEETAQKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACKIYAEMIGNVMIDARSSGKYYHFVRLMGRAASHITLECALQTHPNITLIGEEVPAYDKFSQTLYNVGIILSGGPAPGGHNVISGLYDGLKTNNKDSRLFGFILGPGGLINHEYKELTADIIDEYRNTGGFDLIGTGRTKLEKEEQFEKGLEIMKKLDIKALVIVGGDDSNTNAAILAEYYQAKQAGIQVIGCPKTIDGDLKNDIVEASFGFDTTCKIYSEIIGNIERDCNSTRKYWHFIKLMGRSASHIALECALQTQPNICIISEEVAAKNQTLDDIVTYIAKAVAERAERGLNFGTVLIPEGLIEFIPAMKSLIAELNDLLADTTEGQTAKQSEQRNWVISRLSTKNAAIFASLPESVAAQLTLDRDPHGNVCVSRIETEVLLSSMVGMKLAQWKAEGKYVGKFATQHHFLGYEGRCGFPSNYDADYCYALGCTAAQ",
    "response#2": "GVLTGGGDCPGINAVIRAIVRRGIEFHGFEIIGVRDGWKGLLELEVEPLTLDKVSGILYRGGTILGTSRTNPGKVENGYDIIRDNFVKLGLDGLIAIGGEDTLGVAYKLYEMGVPVVGVPKTIDNDLSATDLTFGFDTAVNVVMEAIDRLHTTAESHHRVMVVEVMGRHTGWIAVYGGLAGGADAILIPEEPFEIEEVCRMVQRRYEKGKRFSIIVVAEGACPKGVDKYFTRGGVDEFGHYRLGGVGEALAQEIERRTGFETRAISLGHIQRGGCPTAYDRILATRLGVAAVDLIEKGEFGRMVSLQGNEIISVPLKEAVGVLKTVDKDLYSLASVFFG",
    "response#3": "GVLTGGGDCPGINAVIRAIVRRGIEFHGFEIIGVRDGWKGLLELEVEPLTLDKVSGILYRGGTILGTSRTNPGKVENGYDIIRDNFVKLGLDGLIAIGGEDTLGVAYKLYEMGVPVVGVPKTIDNDLSATDLTFGFDTAVNVVMEAIDRLHTTAESHHRVMVVEVMGRHTGWIAVYGGLAGGADAILIPEEPFEIEEVCRMVQRRYEKGKRFSIIVVAEGACPKGVDKYFTRGGVDEFGHYRLGGVGEALAQEIERRTGFETRAISLGHIQRGGCPTAYDRILATRLGVAAVDLVARGAHGTMVALQGDRVVPVPLEAAVEALKTVDVDLYLVAQTLLGRGS"
  },
  {
    "reference": "MEKEIVVVTGPTASGKSAACDIIADDLNCRVINCDSKQIYHGVPILTDQPVSVRDIHKLYGYVAPTQNYSAGLWLQDVKREVQQAWDDNVLPVITGGSGMYINSLVNGISDIPAIDPEVRKTARNLLESLGNAAFYEALVARDSNAARLHHNNTHQILRAFEVLEQTGTSIFTWWERSPRIKPFENCEVLVLLPPRNKLYDKINRRFLTMMQTDAISEVKYLMSLNLPMHAPVMKAHGAPEIVQYLQGKIEFMEAVEIAQKNTRHYAKRQCTWFRTQLPCDTRFFSSQNEIIDHVLAQYASKYPRTHTRPCN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein must be able to perform ATP binding, tRNA dimethylallyltransferase activity under specific conditions.2. For general function, the protein need meet that Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).3. The designed protein should have a high specificity for ATP, substrate binding, with minimal off-target effects.4. The protein should have a fast turnover rate for adenosine(37) in tRNA + dimethylallyl diphosphate =   diphosphate + N(6)-dimethylallyladenosine(37) in tRNA.5. The protein must bind to Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MKRLILVVGPTGTKKSYLANMLAKKLNVPIISADAYQVYKELNAGVNKPSEKTLSEIKYHFISNISIFDEWSIAHFNKRAKEILEQAPDWTIVCGGSHLYVNSLINDYQLEKQELDTDLFDALDKLDNQEIFNQLCEYDFQEAIKIGKNNRKRLLRALYLFKKYGKKAKNDSKKFDYVVVKCMSDKEKLFPYLSKRLVP",
    "response#2": "MSKRLILVVGPTGTKKSYLANMLAKKLNVPIISADAYQVYKELNAGVNKPSEKTLSEIKYHFISNISIFDEWSIAHFNKRAKEILEQAPDWTIVCGGSHLYVNSLINDYQLEKQELDTDLFDALDKLDNQEIFNQLCEYDFQEAIKIGKNNRKRLLRALYLFKKYGKKAKNDSKKFDYVVVKCMSDKEKLFPYLSKRLER",
    "response#3": "MKRLILVVGPTGTKKSYLANMLAKKLNVPIISADAYQVYKELNAGVNKPSEKTLSEIKYHFISNISIFDEWSIAHFNKRAKEILEQAPDWTIVCGGSHLYVNSLINDYQLEKQELDTDLFDALDKLDNQEIFNQLCEYDFQEAIKIGKNNRKRLLRALYLFKKYGKKAKNDSKKFDYVVVKCMSDKEKLFPYLSKRLKRLILVVGPTGTKKSYLANMLAKKLNVPIISADAYQVYKELNAGVNKPSEKTLSEIKYHFISNISIFDEWSIAHFNKRAKEILEQAPDWTIVCGGSHLYVNSLINDYQLEKQELDTDLFDALDKLDNQEIFNQLCEYDFQEAIKIGKNNRKRLLRALYLFKKYGKKAKNDSKKFDYVVVKCMSDKEKLFPYLSKRLE"
  },
  {
    "reference": "MDKHKDRIESMRLILQVMQLFGLWPWSLKSEEEWTFTGFVKRNYRFLLHLPITFTFIGLMWLEAFISSNLEQAGQVLYMSITEMALVVKILSIWHYRTEAWRLMYELQHAPDYQLHNQEEVDFWRREQRFFKWFFYIYILISLGVVYSGCTGVLFLEGYELPFAYYVPFEWQNERRYWFAYGYDMAGMTLTCISNITLDTLGCYFLFHISLLYRLLGLRLRETKNMKNDTIFGQQLRAIFIMHQRIRSLTLTCQRIVSPYILSQIILSALIICFSGYRLQHVGIRDNPGQFISMLQFVSVMILQIYLPCYYGNEITVYANQLTNEVYHTNWLECRPPIRKLLNAYMEHLKKPVTIRAGNFFAVGLPIFVKTINNAYSFLALLLNVSN",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be involved in signal transduction, detection of chemical stimulus involved in sensory perception of smell by localizing to dendrite membrane, plasma membrane and exhibiting olfactory receptor activity, odorant binding.2. For general function, the protein need meet that Odorant receptor which mediates acceptance or avoidance behavior, depending on its substrates3. The Cytoplasmic, Extracellular topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.4. The protein should contain a Helical; Name=6, Helical; Name=4, Helical; Name=3, Helical; Name=5, Helical; Name=1, Helical; Name=2 membrane-spanning region. The designed protein sequence is ",
    "response#1": "GNLLIVVTVTVSETLGSPMSFFLAGLTFIDIIYSSSISPRLISDLFFGNNSISFQSFMAQLFIEHLFGGSEVFLLLVMAYDRYVAICKPLHYLVIMRQWVCVLLLVVSWVGGFLQSVFQLSIIYGLPFCGPNVIDHFFCDMYPLLKLACTDTHVIGLLVVANGGLSCTIAFLLLLISYGVILHSLKKLSQKGRQKAHSTCSSHITVVVFFFVPCIFMCARPARTFSIDKSVSVFYTVITPMLNPLIYSLRNSEMKRALKRMIK",
    "response#2": "GNLLIVVTVTVSETLGSPMSFFLAGLTFIDIIYSSSISPRLISDLFFGNNSISFQSFMAQLFIEHLFGGSEVFLLLVMAYDRYVAICKPLHYLVIMRQWVCVLLLVVSWVGGFLQSVFQLSIIYGLPFCGPNVIDHFFCDMYPLLKLACTDTHVIGLLVVANGGLSCTIAFLLLLISYGVILHSLKKLSQKGRQKAHSTCSSHITVVVFFFVPCIFMCARPARTFSIDKSVSVFYTVITPMLNPLIYGCLNRQIRGEVLKLLGMRR",
    "response#3": "GNTTIILVSRLEPKLHMPMYFFLSHLSFLYRCFTSSVIPQLLVNLWEPMKTIAYGGCLVHLYNSHALGSTECVLPAVMSCDRYVAVCRPLHYTVLMHIHLCMALASMAWLSGIATTLVQSTLTLQLPFCGHRQVDHFICEVPVLIKLACVGTTFNEAELFVASILFLIVPVSFILVSSGYIAHAVLRIKSATRRQKAFGTCFSHLTVVTIFYGTIIFMYLQPAKSRSRDQGKFVSLFYTVVTRMLNPLISALNY"
  },
  {
    "reference": "MSLASLSVSPGKGRRVVVGMSGGVDSSVTAWLLKQQGYEVIGLFMKNWEDDDDSEYCSTRQDLLDAASVADLVGVDFEYVNFAAEYKDRVFAEFLREYSAGRTPNPDVLCNAEIKFKAFLDHAMTLGAEHIATGHYARVRAIAGAHGREYQLLKALDGSKDQSYFLHRLNQAQLSRTLFPLGEIHKTEVRRIAHDIGLHNAAKKDSTGICFIGERPFREFLNRYLPTEPGDILTPQGQRVGRHHGLSFYTLGQRKGLGVGGVKGSQRDDGTADAWYVARKDLQHNILYVVQGHDHPWLLSNRLQALDASWVAGRAPELRGYGAKTRYRQADAACVLEQASQGAFELSFSQPQWAVTPGQSAVLYDGDVCLGGGIIA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein can be targeted to cytoplasm and perform ATP binding, tRNA binding.2. The protein must exhibit the following characteristics: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34..3. The Nucleophile active site of the designed protein must be optimized for substrate binding and specificity.4. The designed protein should have a high specificity for ATP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "ITGIRPSWAGAPVPEARTGAWFDCALQFRAHGEIVEARARQRADAAGHPGWEIEPARPLRGVAPGQTAVLYRGTRVLGQATC",
    "response#2": "ITGIRPSWAGAPVPEARTGAWFDCALQFRAHGEIVEARARQRADAAGHPGWEIEPARPLRGVAPGQTAVLYRGTRVLGQATL",
    "response#3": "ITGIRPSWAGAPVPEARTGAWFDCALQFRAHGEIVEARARQRADAAGHPGWEIEPARPLRGVAPGQTAVLYRGTRVLGQATI"
  },
  {
    "reference": "MNLTLLLLALIFSPSLIFSLPTANKVKLVKKGLNWDYQNAKIHGVNLGGWFVLEPFITPSLFDIYSKPNDDSQVPVDEYHFTQKLGKDAAQQVLEQHWKTWYKENDFKMMLKYGLNAVRIPIGYWAFKLLDYDPYVQGQVKYLDRALDWARKYNLKVWIDLHGAPGSQNGFDNSGLRDSLGFQQGNNVNFTLEVLEIIGKKYGGPEYEDVVIGIELLNEPLGPSLDLNYLKEFFQQGYQNLRNSGSVQAVIIQDAFQPMGYWDNFLTLDQYWNVVVDHHHYQVFSAGELQRSIDDHITVACNWGWDAKKEYHWNVAGEWSAALTDCARWLNGVGRGARFSGDFDNSPYFGSCDCYVNIATWPSEYRTNVRRYIEAQLDAFEQTGGWFFWNWKCENAIEWDLQGLITAGVFPYPFYNRQFPNQCGF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Design the protein with a signal peptide incorporated.2. A protein with glucan exo-1,3-beta-glucosidase activity is needed to enhance carbohydrate metabolic process in a specific context.3. For general function, the protein need meet that It could also function biosynthetically as a transglycosylase .; Beta-glucanases participate in the metabolism of beta-glucan, the main structural component of the cell wall4. The protein with the reaction Successive hydrolysis of beta-D-glucose units from the non-   reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose., the Proton donor must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "HPVTPAGVSTDSLSSPIETNKFFDNNLLGSRTNFMYADPFRYWWQSSDTMGGICIAHTDDNQRVMDTDDTIPSYYYEPIGICSLGFGASGITSNTDPIVDEIDQMSARFTFSWDSSSMQLTLTEGMAVTTAVYTNAIPQIFSSTLYINDFVEVPGTSAVQKYRVTMSDNHVWLIYIYGDSLTLTESTSQMLVGSNTFNGYIQIAKIPLGDGTAEALYDTYAGVYITGISISGYVEDAVGYYSFDFTTAGDTSVEPLFFLLPHQVDTAVSGTKVTSIVLASLVSGDMNAAAGNSITFAEAIPQDIGFLPWSV",
    "response#2": "LSQHWSTFITQDDFQQIAQAGMNHVRIPIGYWAVSSLPDEPYVDGQLEYLDNAISWAREAGLKVVIDLHGAPGSQNGFDNSGRKGPIAWQQGDTVSQTVDAFRALAERYLPQSDVVTAIEALNEPNIPGGVSEAGLRDYYNQIADVVRQIDPGTSVFLSDGFLSTESWNGFKTGEDVVMDTHHYEMFDNYLISLDIDGHVKSACDFGKQIEGSDKPVVVGEWSGAVTLSCVVKDSVDAEKYGELFGQVCGYGGGICDGIARNATAGSYGAYSVCTSKDQLSYVFDRYYKSQKKAASACDFAGAAIEFFPRSAKRDGVTHATFRDAEGWLPYIQQLGFDTVYLPPIHPIGRTARKGKNNSLAAAADDVGSPWAIGASEGGHKAVHPKLGTLEDFRHFVETAQAHGIEVALDLAFQCSPDHPYVKEHPEWFQHRPDGTIKTAENPPKRYEDIVNFDWMGPARESLWAELESVVLHWVKQGVNTFRVDNPHTKPTQFWAWLIRRVQEAHPQVLFLSEAFTRPKVMKALGKVGFTQSYTYFTWRNFKGELQEYLEEITQPPVSDYFRGNLWPNTPDILPEFLQNAGPGAFRLRAALAGTLSSVWGMYCGYELCEGRPIPGKEEYTDSEKYQLVAWDLDRPGNIRDWIARLNAARTPALYTGNWFGEPFLQPMFYMYPEDKNTFSNDMQFFYGDSILVSPVHDVSQTSVEAYFPKDIFYDWNTGDVLRGRGAKVTLSNISVTDIPIHIRGGSIFMVVPA",
    "response#3": "IKEESHDEIVSALSKDVESLDSSSITTTESYFYGKLIARAARLVLIAEELNYPDVIPKVRNFLKETIEPWLEGTFSGNGFLHDEKWGGIITQKGSTDAGGDFGFGIYNDHHYHLGYFIYGIAVLTKLDPAWGRKYKPQAYSIVQDFLNLDTKLNSNYTRLRCFDPYVLHSWAGGLTEFTDGRNQESTSEAVSAYYSAALMGLAYGDAPLVALGSTLTALEIEGTKMWWHVKEGGTLYEKEFTQENRVMGVLWSNKRDTGLWFAPAEWKECRLGIQLLPLAPISEAIFSNVDFVKELVEWTLPALDREGGVGEGWKGFVYALEGVYDNESALQKIRNLKGFDGGNSLTNLLWWH"
  },
  {
    "reference": "MIELGVNIDHIATIRQARRTYEPDPVWGAVEAHLGGADGITVHLREDRRHIQDSDVRRLRETTQIKLNLEMAATDEMVGIACGLKPEMAMLVPEGRHEVTTEGGLDILAQEARLKGVISRLADAGIVTSVFIDADPEQIEAAARIGASVCEIHTGPYAHAFYDKGRDAEAPAVLAEIAKIRKAGQTIRHLGMRFNAGHALNYYNVQPLARLAGIRELHIGHAIVSRAVFVGLREAVREMKVLMREAAERGE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should interact with enzymes and substrates within the pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 5/5 pathway to enhance or regulate its activity.2. The Proton donor active site of the designed protein must be optimized for substrate binding and specificity.3. The protein design should aim to mimic or enhance the function of natural proteins involved in pyridoxine biosynthetic process, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "LPGKAWNERDAGGLNSLESLNLWAKEHYDLFRDDELFRQQGLTTEGFDSLRRNYRKESPRREFINQVVTCHNSEQYIQFLQLN",
    "response#2": "LLPIAPVPNVSLSRKWDDATLFSLIQLIYDIRKDDALFRRNMVVTKGNEAQMATAFDQMRKNYWDRREYSAITVTGKVGFGVESLAKLF",
    "response#3": "LLPIAPVPNVSLSRKWDDATLFSLIQLIYDIRKDDALFRRNMVVTKGNEAQMATAFDQMRKNYWDRREYSAITVTGKVGFGVESLAKLL"
  },
  {
    "reference": "MVKVLVASRNQGKVRELEQVLRELNIEGVELVSLHDAPPYPDPVEDGLTFVDNALLKARAGAKATGLPCVADDSGLSVKALNGMPGILSARWSGNHGDDQANNDLLLAQMADINERAAAFVSCCALAAPDGQEFTAEGRWEGELLREPRGENGFGYDPLFQPADADGRSSAELTPEEKNARSHRGKALRALAPHIAALR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.2. The protein should have a high affinity for substrate, Mg(2+).3. A protein with XTP diphosphatase activity, ribonucleoside triphosphate phosphatase activity, dITP diphosphatase activity, metal ion binding, nucleotide binding functions is required to enable nucleotide metabolic process.4. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "MERFLIVFSGGQDSTTCLFYAKKNFDQVVCVTFDYGQRHAIELEVAKKLAAKL",
    "response#2": "MPK",
    "response#3": "MPIGVPKVPFRNPGEPDAACARQLL"
  },
  {
    "reference": "MESISQLQPPMGVNFLNIAPTYIPNTKVECHYTTPFGMKRSTRDWIGIFKVDTSSIRDYETFVWAVPPESSGERSVSHCSVQFQAYYLPRPGEQQYHFRYVDQYGSVRGCSDAFVFGEPRPMEELVTLEDEDSCMDMLLVVPKATFLQNQLEMAQQERNDLMRARLALEEEVILKEKRINHLEATLETSEKSCFSLKEQCQDLVMREQLAIEEQSLLSCQEAELRERILQLQGEIKIMNKKMQEKDRELEGTVAIKLSLENKKVVLKQHLAETTVEIKRYQLQVDSLREKLRSTQDMLSASQQKALLMGEELAAISSIRDRTISDLHKSRLETADLAIKVSDLSVKYKEGMGQWWQEKTALNHSMEAKRDQIVNLKAEKLSLENSLQAERSQRQALQCKLNQETDARQVQLSENRRQLSELKSALKVAQMEKQQLREERQGILQYARSLEERLDKLADELWKEDKMLMEEITDLNPPTLPVDHSDSDDESPGDERVSQQLEACSLDEQDLTINLPGFPCELYKVVINQPAPIACQLQPLPEDNSDSW",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein localizes to the nucleus, cytoplasm.2. The protein must exhibit the following characteristics: May function as a coactivator for aryl hydrocarbon and nuclear receptors.. The designed protein sequence is ",
    "response#1": "MSTLNMLGGRQVVAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFLMAFALGWRSYKQSNGSLLCFAPDLIINEQRMNLSCMYDQCEHMLMVGNELARLQVSYEEYLCMKTLLLLSTIPREGLKSQALFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLLDSMHDVI",
    "response#2": "MLGGRQVVAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFLMAFALGWRSYKQSNGSLLCFAPDLIINEQRMNLSCMYDQCEHMLMVGNELARLQVSYEEYLCMKTLLLLSTIPREGLKSQALFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLLDSMHDVL",
    "response#3": "MSTLNMLGGRQVVAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFLMAFALGWRSYKQSNGSLLCFAPDLIINEQRMNLSCMYDQCEHMLMVGNELARLQVSYEEYLCMKTLLLLSTIPREGLKSQALFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLLDSMHDVR"
  },
  {
    "reference": "MKGHVARKRFGQNFLVDQGIIAAIISAVDPKRDETVVEIGPGLGAITEPLMARVDHLHVIEIDRDLIARLKKQHTPERMTVHEGDALAFDFASIGKNLRLVGNLPYNISTPLLFHLAEYVDVVHDMHFMLQKEVVERMVAEPGNADFGRMSVMLQYRFYLEWLIDVPPESFDPPPKVQSAVVRLIPKPVSELNAKSQEKLSQVALTAFSQRRKMLRNTMKTLLSDAAFTELGIDPTCRPEDVSVEDYVRIANYLS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein can be targeted to cytoplasm and perform 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity.2. The protein should be designed to catalyze the following reaction adenosine(1518)/adenosine(1519) in 16S rRNA + 4 S-adenosyl-L-   methionine = 4 H(+) + N(6)-dimethyladenosine(1518)/N(6)-   dimethyladenosine(1519) in 16S rRNA + 4 S-adenosyl-L-homocysteine. The designed protein sequence is ",
    "response#1": "MAEKIVGTLTAPPEAHVVEVGAGTGVLTERLAERHDRLTALEIDERAVEVLRERVPEADVRETDVRETDWAALADEKGGPLRVISNTPYYLTSPILFALLGQRDCLAEAVLTMQKEVAERIVAEPSTKAYGILSVLLQLFAEPTLCFTVPPQVFSPQPDVTSAVVRIRFGD",
    "response#2": "MAEKIVGTLTAPPEAHVVEVGAGTGVLTERLAERHDRLTALEIDERAVEVLRERVPEADVRETDVRETDWAALADEKGGPLRVISNTPYYLTSPILFALLGQRDCLAEAVLTMQKEVAERIVAEPSTKAYGILSVLLQLFAEPTLCFTVPPQVFSPQPDVTSAVVRIRFGE",
    "response#3": "IAKRIVKEADLKPDDVVVEIGAGAGTLTEELAKTGARVIAYEIDEGLAPILQERLSKYPNVELRFEDFLKARDVPEEAICVSNIPYSVTGPIMEKIIEWRFKKAIVMVQKEVGERILSKPGRKSYGYLSVVVQTFYEVRKLFDVSRSYFVPNPEVDSVVVEMKRKL"
  },
  {
    "reference": "MESFMGMKAKAEALDAKDPLGAFRERFVIPQGRVYLDGNSLGLPARDSIEGVLRVLEEWKTLNIDGWLEGRIPWFNLPEEMGRRMAPLVGALPQEVVMSGSTTSNLHSLVLTFYQPEGKRRKILADELNFPSDLYALASEIRLKGGDPATDLVLARSKDGSTLEEEDLIALMDETVSVVLLPSVLYRSGQLLDMERLTRAAHEKGIFIGFDCAHSAGSVPHRLHDWDVDFAFWCSYKHLNGGPGSPAFLYLNERHFGRDPGLAGWFGYVKEKQFDMKIDFEHAQSAGGWQLGTPSVLSAATLEAALKIQEEAGIDRIREKSLRLTDFLIELVDGFLSDEPYEYSIGSPRDHGRRGGHLAIEHPREAWRICCALKARGIVPDFRPPRVIRVAPVALYNTFTEVWELARALKEIIEKREFERFPKERSAVS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. We require a protein that can specifically bind to the metabolites produced in the Cofactor biosynthesis pathway. The designed protein sequence is ",
    "response#1": "FSFADFGTRRRASKAWHEEVLLTLKEQLPASFSGTSSLHFARTLGLPPIGTMAHEWFQSWQAVTRLADAQKAALEGWVQEYRGRLGIALTDCYSMDSFIRDFSDPYFGKLYDGLRHDSGCPFVWAEKAILMYQQMGIDPLSKTLVFSDGLNFPRMLEIWKRFKGRAKISFGIGTNLTNDVGNVPLNIVIKMIAANGRPIAKISDEPGKSMCEDLGYLQYLASQYL",
    "response#2": "FSFADFGTRRRASKAWHEEVLLTLKEQLPASFSGTSSLHFARTLGLPPIGTMAHEWFQSWQAVTRLADAQKAALEGWVQEYRGRLGIALTDCYSMDSFIRDFSDPYFGKLYDGLRHDSGCPFVWAEKAILMYQQMGIDPLSKTLVFSDGLNFPRMLEIWKRFKGRAKISFGIGTNLTNDVGNVPLNIVIKMIAANGRPIAKISDEPGKSMCEDLGYLQYLASQYL",
    "response#3": "FSFADFGTRRRASKAWHEEVLLTLKEQLPASFSGTSSLHFARTLGLPPIGTMAHEWFQSWQAVTRLADAQKAALEGWVQEYRGRLGIALTDCYSMDSFIRDFSDPYFGKLYDGLRHDSGCPFVWAEKAILMYQQMGIDPLSKTLVFSDGLNFPRMLEIWKRFKGRAKISFGIGTNLTNDVGNVPLNIVIKMIAANGRPIAKISDEPGKSMCEDLGYLQYLASQYL"
  },
  {
    "reference": "MLINNKKVKLFSLNSNPQLAEEVSDYTGIPLSKCHVERFADGEVSIDIEETVRGHHVFVVQPTNAPVNENLMTLLIMIDALKRASARTINVIMPYYGYSRQDRKARARQPISAKLVADLLQAAGAARVLSMDLHASQIQGFFNIPIDNFVALPSITKYFLDKKFEKDIVVVSPDHGGATRARKLAEYLQAPIAIIDKRRPKPNVAEVMNVIGEVNDKVAIIIDDMIDTAGSITSAATALKDRGAVEVYAAATHPILSGPAIKRLTDSVIEEVVVTNTIALPEEKQIKKIVQLSIGQLLGQGVLNIIYDKSVSELFKVDNGVE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a high affinity for Mg(2+), D-ribose 5-phosphate.2. A protein with ATP binding is needed to enhance 5-phosphoribose 1-diphosphate biosynthetic process in a specific context.3. The designed protein should contain one or more Ribose-phosphate pyrophosphokinase N-terminal domains that are essential for its function.4. The protein design should consider the endogenous proteins and enzymes present in the 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MKIFAGNATPKLANLIAKRLYTQLGEATVSRFSDGEISVQINENVRGDDIFIIQSTCAPTNDNLIELVVMIDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKIVADFLSSVGQARAVDLHAAQIQGFFDTPVDLHAQQIQGFFDIPVDLHAGQIQGFFNIPVDLHSGQIQGYFDIPFASPVILDYLKSKELKDIVVVSPDVGGVARARAFAKKLNDAPLAIIDKRRQAHNVAEVMNVIGDVKGKTAVLVDDMIDTAGTISEGARLLRKEGARQVYACATHAVFSPPATDRLSGGLFEEVIVTNAVPLGPGASLMIVSGQGYPELAERIADRLDMDLSGVELVKFPGGELYARYLESVRGADVFIIQSLCEPVNENLMQLLIMIDAAKRASAKRIVAVIPWYAYSRQDRKTKPREPITARLVANMIQAAEAVARAS",
    "response#2": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGCDRAITIDLHAPQLQGFFNIPVDHLTAPLLADYFLRNNLEEGAVVVSPDHGGVNRARKLAEFLDCPIAIVDKRRPKANVAEVMNIIGDVKGKRAIIIDDMIDTAGTITLAAQALIDAGATEVYASATHAVLSGPAIKRLNDSPIKNLVLTDSVPIPEGRQLSQLSIAPLFAEAIRRIHRNESVTSLFE",
    "response#3": "LSDYFLEKALKNLVVVATDVGIAKRARDLAARLNVPLAIVEKRRVGNVDKSETLTVIGDVKGKRALLVDDEVDTGGSIANAAVTLKKHGAIEIYACCTHPILSGSGVKRIAESIIKELVVTDAAPVMYQYLKQMQVGDLVVVSPDVGGLKMAYAYSQVLSAGLAIVAKRRKSATEVESLTVIGEIRGKNVLMVDDLTETAGTLTTAAALLKRKGAKRILACVSHVLLNDLGVERLRKSSIDELITTDFDYVGAEDLLAGYFTDAGFKDADDVVVVSPDHASVSRARAYAERLDSPIAIIDNRAPEDIHTIPESVIGNVKGKRAILVDDMIDTGIKLQISAETLIEAGATEVYAVATHAVFSGDAALRLQEAPIKKTVVTDFDYVG"
  },
  {
    "reference": "MALINFDLLAELPLAYQAFAPTVDVLPLIPLFFFLLVFVWQAAVGFR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should contain a Helical membrane-spanning region.2. For general function, the protein need meet that One of the components of the core complex of photosystem II (PSII) The designed protein sequence is ",
    "response#1": "MSESSPWETIQFEDQSNALDIDFIDNNHGFLVGSNRLIMESNDGGKSWEKRSLDIAAEENFRLLDIDFKGSEGWLIGQPSLVMHTIDEGKNWTRLSLGKLPGQPFLVSTVDDGVAELATTSAAIYTTSNSGETWEAKVSDPSEQGGIRDLRRTSNGDYVSVSSLGNFFSTLESGSDTWIAHQRASSKRVQGIGFNPDGNLWMLSRGAEIRFNDDSNDLESWTKPIVPILNGYNYLDMGWDPEGNIWAGGGNGTLIVSKDDGKTWDSDPVASNLPTNFIKIQFLEKDELDAKKGFILGERGYILRG",
    "response#2": "MSESSPWETIQFEDQSNALDIDFIDNNHGFLVGSNRLIMESNDGGKSWEKRSLDIAAEENFRLLDIDFKGSEGWLIGQPSLVMHTIDEGKNWTRLSLGKLPGQPFLVSTVDDGVAELATTSAAIYTTSNSGETWEAKVSDPSEQGGIRDLRRTSNGDYVSVSSLGNFFSTLESGSDTWIAHQRASSKRVQGIGFNPDGNLWMLSRGAEIRFNDDSNDLESWTKPIVPILNGYNYLDMGWDPEGNIWAGGGNGTLIVSKDDGKTWDSDPVASNLPTNFIKIQFLEKDELDAKKGFILGERGYILRR",
    "response#3": "MSESSPWETIQFEDQSNALDIDFIDNNHGFLVGSNRLIMESNDGGKSWEKRSLDIAAEENFRLLDIDFKGSEGWLIGQPSLVMHTIDEGKNWTRLSLGKLPGQPFLVSTVDDGVAELATTSAAIYTTSNSGETWEAKVSDPSEQGGIRDLRRTSNGDYVSVSSLGNFFSTLESGSDTWIAHQRASSKRVQGIGFNPDGNLWMLSRGAEIRFNDDSNDLESWTKPIVPILNGYNYLDMGWDPEGNIWAGGGNGTLIVSKDDGKTWDSDPVASNLPTNFIKIQFLEKDELDAKKGFILGERGYILRR"
  },
  {
    "reference": "MEFPFDINQLFSERVSVLDQTLVAGRTSTGMPQLQARIATVIDELGRASAKAQQLPASITSASKLQSQRHQLYLLKDGESNGGRGVAVGFLKVGYKKLFLLDRQGVHIEAEPLCVLDFYIAEGVQRHGYGLELFNFMLQHKNLEPVVMAYDRPSPKFQSFLAKHYSLTPSVPQANNFVVFEGFFLNRPAAQLRKVPLRKPNGDIKPYSLMEREVVLREQLARPWPFAPPHSPQRSVSSRCSHSLSVGSSPSRGLPRTFPASAPGGSRDPSPQSPLIERCRARRSSSLNRSHLGFH",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should contain one or more N-acetyltransferase domains that are essential for its function.2. The designed protein should have tubulin N-acetyltransferase activity within clathrin-coated pit to regulate regulation of microtubule cytoskeleton organization, neuron development, alpha-tubulin acetylation.3. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.4. The protein must exhibit the following characteristics: Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules; May be involved in neuron development.; Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity.5. The protein should be able to bind acetyl-CoA ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MEFSFDINQLFPERVSILDQTLVAGPNSVLRPDLQAHIATVIDELGRASAKAQQLPAAITSASKLQSQKHQLYLLKDGESNGGRGVVVGFLKVGYKKLFLLDRQGEHVEAEPLCVLDFYIAQSMQRHGYGLELFDFMLQHKKLEPVLMAYDRPSPKFLSFLAKHYCLTPSVPQANNFVVYEGFALFPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIIDELGKASAKAQNLSAPITSASRMQSNRHVVYILKDSSARPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFALFPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIIDELGKASAKAQNLSAPITSASRMQSNRHVVYILKDSSGRPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFSFDINQLFPERVSILDQTLVAGPNSVLRPDLQAHIATVIDELGRASAKAQQLPAAITSASKLQSQKHQLYLLKDGESNGGRGVVVGFLKVGYKKLFLLDRQGEHVEAEPLCVLDFYIAQSMQRHGYGLELFDFMLQHKKLEPVLMAYDRPSPKFLSFLAKHYCLTPSVPQANNFVVYEGFFLNRSAAQLRPFDVDALFPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIIDELGKASAKAQNLSAPITSASRMQSNRHVVYILKDSSARPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPAPS",
    "response#2": "MEFSFDINQLFPERVSILDQTLVAGPNSVLRPDLQAHIATVIDELGRASAKAQQLPAAITSASKLQSQKHQLYLLKDGESNGGRGVVVGFLKVGYKKLFLLDRQGEHVEAEPLCVLDFYIAQSMQRHGYGLELFDFMLQHKKLEPVLMAYDRPSPKFLSFLAKHYCLTPSVPQANNFVVYEGFPFDVDALFPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIIDELGKASAKAQNLSAPITSASRMQSNRHVVYILKDGSARPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSPKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPAPSTLFSERVSILDQTLVAGRKSAGRPDLQAHIATVIDELGRASAKAQQLTAPITSASKLQSQRHQLYLLKDAESNGGRGVVVGFLKVGYKKLFLLDLQGVHIETEPLCVLDFYIAENLQRHGYGQELFDFMLQHKNLEPVLMAYDRPSPKFLSFLAKHYCLTRSVPQVNNFVVFEGFPFDVDALLPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIVDELGKASAKVLESLHGAQHLPAPITSASRMQSNRHVMYVLKDTSARPAGKGAIVGFLKVGYKKLFVLDDREAHNEVEPLCILDFYIHESLQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHMEFPFDLNALLPERISVLDHNLTAGRKSLGRPDPQRQISTVIDELGKASAKAQQLAAPITSAPKLQSNRHHLYLLKEGESNGGRGVAVGFLKVGYKKLFLLDQQGAHVEIEPLCVLDFFVTENLQRHGYGLELFSYMLQHKRVEPVRMAYDRPSPKFLSFLEKHYGLKDSVPQVNNFVVFDGF",
    "response#3": "LLPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIVDELGKASAKVLESLHGAQHLPAPITSASRMQSNRHVMYVLKDTSARPAGKGAIVGFLKVGYKKLFVLDDREAHNEVEPLCILDFYIHESLQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFPFDINQIFSERISILDQTLVASRKSAGRPDLQANTATVIDELGRASAKAQQLLAPITSASKMQSQKHQLYLMKDGERNGGRGVVVGFLKVGYKKLFLLDRQGVHIEAEPLCVLDFYIAENLQRHGYGLELFEFMLQHKNLEPVMMAYDRPSPKFLSFLAKHYCLMQSVPQVNNFVVFEGFFLNRAAAQLRNQLFSERISILDQTLVAGRKSTGRPDLQAHIATVIDELGRASAKAQQLTAPITSASKLQTQKHQLYLVKDGESSGGRGVVVGFLKVGYKKLFLLDRQGVHVEAEPLCVLDFYVAENLQRNGYGLELFNFMLQHKNLEPVLMAYDRPSPKFLSFLAKHYCLTQSVPQVNNFVVFEGFTLFSERVSILDQTLVAGRKSAGRPDLQAHIATVIDELGRASAKAQQLTAPITSASKLQSQRHQLYLLKDAESNGGRGVVVGFLKVGYKKLFLLDLQGVHIETEPLCVLDFYIAENLQRHGYGQELFDFMLQHKNLEPVLMAYDRPSPKFLSFLAKHYCLTRSVPQVNNFVVFEGFPFDVDALFPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIIDELGKASAKAQNLSAPITSASRMQSNRHVVYILKDSSGRPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPAPS"
  },
  {
    "reference": "MHQPPLPSLTAADPEIARLVEGEAHRQFGKIRLIASENYVSRAVLEATGTVLTNKYSEGYPGRRYYEGQQFVDPVEALAVDRAKALFGVEHANVQPYSGSPANLAVYLAFLNPGDTVMGMALPMGGHLTHGWAVSATGKWFRSAQYGVRKETGRVDLDEVREIALRERPKVIFCGGTAIPRTIDFPAFAAIAAEVDAVLVADIAHIAGLIAGGAHPSPVGHAQVITTTTHKTLRGPRGAMILSDAAHAKAVDKAVFPGLQGGPHNHTTAAIAVALGEASAPGFRTYAHNVVANAQALAAALAQRGFDLVSGGTDNHLVLVDLAGKAIGGKPAAQALDRAGIEANYNTVPYDPRKPFDPSGLRLGTAAITTRGLGPDQMPQVAAWIEAAVAAAGDDAALGRIAAEVAELMAGHPMPGWA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must be engineered to have a cooperative binding mechanism with pyridoxal 5'-phosphate to ensure proper regulation.2. The designed protein must have at least one Serine hydroxymethyltransferase-like domain.3. The protein design should take into account the physiological conditions of the tetrahydrofolate interconversion pathway.4. A protein that contributes to tetrahydrofolate interconversion, methylation, glycine biosynthetic process from serine by functioning in cytoplasm with glycine hydroxymethyltransferase activity, methyltransferase activity, pyridoxal phosphate binding.5. The protein must exhibit the following characteristics: This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules; Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier; Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism..6. The (6S)-5,6,7,8-tetrahydrofolate binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "LQKQDKEVFDAIRLELGRQRNNIELIASENFVSEQVMEAMGSVLTNKYAEGYPRKRYYGGCEYVDIVEDLARDRAKQLFGAEYANVQPHSGAQANMAVYQAVLKPGDTVLGMNLAHGGHLTHGSPVNFSGLLYNFVEYGVSKETKQIDYDIVRETALAHKPKLIVAGASAYPRAIDFAKFREIADEVGAYLMVDMAHIAGLVAAGLHQNPVEYADFVTTTTHKTLRGPRGGLILAKAEWETKINKAIFPGIQGGPLMHVIAGKAVAFGEALQPEFKNYAAQIIKNSKQLAKTLEENGVQVLTGGTDNHLLLIDLNHLSITGKAAEEALDSVGITVNKNSIPFETESPFVTSGIRVGTAAITTRGFDEAATQIVGELITEALTAIRDNAKADLEGARVRQMLDYVTCADLSALKEGESTAVCLETPEGHVEGALTCVTWDHYRLSLPAKQAAATVTWLRDLSDGYVSLRLDGAPDYSAMRIPGDLEGARVRQMLDYVTCADLSALKEGESTAVCLETPEGHVEGALTCVTWDHYRLSLPAKQAAATVTWLRDLSDGYVSLRLDGAPDYSAMRIPGDLEGARVRQMLDYVTCADLSALKEGESTAVCLETPEGHVEGALTCVTWDHYRLSLPAKQAAATVTWLRDLSDGYVSLRLDGAPDYSAMRIPGS",
    "response#2": "ETYDPELFAAIAAENQRQQDHIELIASENYVSYAVMEAQGSQLTNKYAEGYPSKRYYGGCEHVDVAEQLAIDRVKKLFSAKYANVQPHSGSQANQAVYASVLKPGDTILGMSLAHGGHLTHGASVNASGKLYNAITYGLDENEVLDYAEVERLALEHKPKMIVAGASAYSLEIDWARFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVPFADFVTTTTHKTLRGPRGGVILARDLTHEKALNSAVFPNLQGGPLMHVIAAKAVAFKEALQPEFKQYTKQVKINAAAMAEELTKRGLRIVSGKTESHVFLLDLRAKHITGKEAEAALGKAHITTNKNAIPNDPEKPFVTSGIRIGSAAITTRGFDEADARALANLIAKGIGDAKVDLKVAETDPDLYAAIVAEEKRQRNNIELIASENFASPAVMEAQGSVLTNKYAEGYPGKRWYGGCENVDVVEQLAIDRAKKLFGAEHANVQPHSGSQANTAVYFSVLKPGDKILTMDLSHGGHLTHGHKANFSGKFYEVVHYGVSKEDEQIDYDHLAELARETKPALITCGASAYSRVIDFARMAQIAREVGAYLFVDMAHIAGLVAAGVHPNPVPHADFVTSTTHKSLRGPRGGIILCKEEFAKKIDAQVFPGIQGGPLMHVIAAKAVCFGEALKPDFKDYQAQIVKNAQALAARLTSHGYRIVSGGTDNHVMMVDLRPKGLNGADASHALDEAGITVNKNSIPFDTGTPMKPSGIRIGTPAVTTRGMKEKDVEQVADFIAVALAKGNDAELEAIKTEVAELTEKFPLYEGLED",
    "response#3": "LQKQDKEVFDAIRLELGRQRNNIELIASENFVSEQVMEAMGSVLTNKYAEGYPRKRYYGGCEYVDIVEDLARDRAKQLFGAEYANVQPHSGAQANMAVYQAVLKPGDTVLGMNLAHGGHLTHGSPVNFSGLLYNFVEYGVSKETKQIDYDIVRETALAHKPKLIVAGASAYPRAIDFAKFREIADEVGAYLMVDMAHIAGLVAAGLHQNPVEYADFVTTTTHKTLRGPRGGLILAKAEWETKINKAIFPGIQGGPLMHVIAGKAVAFGEALQPEFKNYAAQIIKNSKQLAKTLEENGVQVLTGGTDNHLLLIDLNHLSITGKAAEEALDSVGITVNKNSIPFETESPFVTSGIRVGTAAITTRGFDEAATQIVGELIARALTPSDEAKLAAERDADPEIAHMLDKEAERQDGGLELIASENFASCAIRAAQGTVLTNKYAEGYPGKRYYNGCEVVDEVESLAIKRACQLFGAEAANVQAHSGSQANMAAYFALIKPGDTVLAMDLAHGGHLTHGHPMNFSGMLYKIVPYGVDKQTEVLNYDEIEKLALENSPKVIVVGASAYPRTLDFKRFREIADKVGAKVFVDMAHVAGLVAAGIHPNPVPYADVVTSTTHKTLRGPRSGLILCKEQYAKDINSKLFPGLQGGPMMHTIAAKAICFHEALQPEFKVYQKQIVKNASHLAAELVKCGFRIVSGGTDNHLMLVDLRPKKSTGKEIALALDKAAITVNKNMIPFDPEKPFIASGIRVGTPAVTTRGMKEAEMTKIAGWIADVLDNVENADVRDKVRAQVAELCGKFPVYGA"
  },
  {
    "reference": "MNKNNISIIILAAGKGSRMKSSTAKVLHKISGKPMIYHIIKASKEISDDITVVVAHQKEHVQKEIQSYFPDIHFCVQDSQNFPGTGGAMKNISPKNEKVLVLNGDMPLLTSKALXGFIDTDSDIIMSVXNVENPDGYGRVIIEDSQVQXIVEQKDCREEELKVXTVNAGIYLFTKAVVKKYIPLLNNTNAQKEYYLTDVIAMANKDALTITPLLVDEQIFKGVNSKKDLSDSEIIMQDSIKRKWMDAGIIMQLPQTIYIEEGVTFEGECILENACRLTGNTFIKDSHIKSGSVIEDSVIKNSDIGPLAHIRPASYIQDSHIGNFVEIKKSSLQGVKAGHLSYIGDAQIDEGTNIGAGVITCNYDGIKKYKTLIGKNVFVGSDCQLIAPVTLEDDVMIAAGTTLXSGTVKQGSLALSRTKLXXIKNFYYKFFAKKSV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein can be expressed in cytoplasm and perform UDP-N-acetylglucosamine diphosphorylase activity without triggering undesirable cellular responses.2. The protein design should consider the endogenous proteins and enzymes present in the Nucleotide-sugar biosynthesis pathway to avoid interference with native metabolic processes.3. The protein should have a low Km value for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.4. The protein must bind to Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MGHRSSSPKVLHTLGGRSMLGHVLAAAAPLAAGVTVVVVGAGRELVREHLAAVAPEALPVVQEEQRGSGHAAAVALAAVPELSGPVLLVNGDAPLLRTTTVAALVEEHLRAGNALTVLTAEVADPTGFGRILRDADGAVAAIVEQKDATAEQLAVDEVNAGILAFDGARLGDALGRLSTDNDQGELYLTDVLAIARGDGRAVAAHVVDDVWQTEGANDRVQLAGLRAELNRRTLEAAMRGGVTLVDPETAYIDPAVRIGTDTVIAPGVHLQGNTSIGRDCRIEPGVVVRDCQVADGVHLKAGSVLEGSQVGAACAIGPMAHLRPGTVLAGHNKLGNFVETKKAILAEGAQASHLTYLGDAEIGRNVNIGCGTITCNYDGVNKHRTIIGDNVFVGSDVQFIAPVRIGSGSLIGAGSTITRDVPEDALAIARSEQRNIEGWVASRKKGTPTAEAAAAARAARDESGE",
    "response#2": "AVILAAGQGTRMKSSRPKVLHEVAGMPMVYYPLQLAAELHCDPRLVVVGHGAATVEAYLADSGAGFVRQEEQLGTGHALRCAAEALAGFNGTLLLLCGDVPLLRRETLDRLLAYHESEQAAVTVLTAEVAEPRGYGRVVRDAAGRVTGIVEQKDANAAERAIREINVGTYAFDAAVLRDGLARLGTTNAQGEKYLTDVIGIARGDGRPVGALVVDDPWLTEGVNDRVQLSALAAEMNRRIVERWQRAGCVIGSDVILGPFAHLRDGAVIADGARIGNFVEVEPGVTIVDPNTTWVDQTVELARDVTLLPNTQLHGATTVGSGTTIGPDTTLTDLEIGENATIIRTHGSLAVVGDGANVGPFAYLRPGTRLGVKGKIGTFVETKNTSIGDGAKVPHLTYAGDATIGEGANIGAGTIFANYDGVAKHPTHVGKHSFIGSNSVIVAPRTIADGAYLAAGTALTEDVGPGEIAVARGRRRNIEGWVLRRRPGTAAAEAAETALGAEAAGTPGADGSGTDGSGGDGSGGDGSGGDGSGGDGSGGDGSGGDGSGGDGSRSSSPKVLHTLGGRSMLGHVLAAAAPLAAGVTVVVVGAGRELVREHLAAVAPEALPVVQEEQRGSGHAAAVALAAVPELSGPVLLVNGDAPLLRTTTVAALVEEHLRAGNALTVLTAEVRRGA",
    "response#3": "VILAAGMGKRMQSALPKVLHPLAGKPLLGHVVDLGRSLAPSQLCVIYGHGGDAVPKAFADQGLSFALQEPQLGTGHAVMQALPHLDDNAPTLVLYGDVPLTSTATLQRLLQAAGQDKLGVLTIVTATLPDPTGYGRILRDAAGRVVAIVEQKDATEAQRQIREINSGIMAMPARRLGDWLTRLSNDNAQGEYYLTDIIAHAVSEGVEVATAQPEAAAEVLGVNDRVQLSELERTHQRRQAEALMTEGVWMLDPGRVYIDVSVRLEAGVRILPGVHLEGSTSVAEGAELGPDCFIRDSRIGPGARIWYSVVRGVSVGTDAQVGPYASLRPGVELGDSAKAGTFVEVKNSVVGARAKVPHLAYVGDTDIGEEANVGAGTITANYDGYNKHRTRIGSRAQIGSNTVLVAPVEVGAEAYTGAGSAITRDVPDGALGVERTSQRNIAGWTRPTKK"
  },
  {
    "reference": "MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETHEGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGAVTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAHPIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAVAETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVIDYLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVLQSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEKAKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVISGMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDVWIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLYDGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTARRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQEEIIKKNKGED",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation.2. The designed protein should be optimized for GTP binding activity in order to improve ribosome disassembly.3. The GTP binding site should be located in a region of the protein that is accessible to the ligand.4. The designed protein should contain one or more tr-type G domains that are essential for its function. The designed protein sequence is ",
    "response#1": "PHVNVGTIGHIDHGKTTLTAAITKVLSKHNPKIAFRSFDSIDNAPEERERGITIATAHVEYETANRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTKEHVLLARQVGVPYVVVFLNKCDAVEDAELIDLVEMEVRELLNKYGFPGDDLPVVRGSALGALNGEAQWEAKIDELMEAVDKNIPLPVDSPLRLPVQDVYKFDDRRIILAGRLDQGQLKVGDPIIVSPANKTSTVKTIESWDSKKDLSA",
    "response#2": "VVTVMGHVDHGKTSLLDAIRKEEVAASEAGGITQHIGAYTITINGEKITFLDTPGHEAFTSMRARGAQITDIVILVVAADDGIMPQTEEAINHCKAANVPMVVAINKMDRAGANPDKVKQQLAEKGLVAEDWGGDTITVPVSAHTKEGIDTLLEMVLYRNIGIFAHVDAGKTTTTERILKLTGKIHKTGEVHDGESTTDFMEQEAERGITIQSAATTCFWKDHRMNIIDTPGHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYANESGVARCIFVNKLDRMGADFYRVVGQVKKVLAANPLVMTLPIGIEDEFKGVVNLLDMKAYIWDDTGLPENYEVVDIPEDMVEKANEYREQLIETAVEQDDDLMMAYMDGEEPSLEDIKRCIRKGTRDLAFFPTYCGSAFKNKGIQLVLDAVIDFLPSRK",
    "response#3": "INGERVDALSLIVHRNQAHYRGRELAEKMRELIPRQLFDVAIQAAIGAHIVARTTVKALRKNVTAKCYGGDITRKRKLLEKQKAGKKRMKQVGSVEIPQEAFLAVLS"
  },
  {
    "reference": "MRIILILERVTVRLSDRKLLFGITGSIASYKAVYIVRHLVKNEGAQIQVVMTKAATEFVRPVVFETVTGSPVYVEMFGEVGFGTVHIDLARESDLLVICPATANIIAKIACGIADDLLSSVALVKGSNMVIAPAMNDQMYLNPITWDNISKLKRLGCVVVECEEGDLACGTTGIGRLAEEKKIILAILRKLREDERLKGKKVVVTAGPTREYIDDIRFISNRSTGKMGFAIAEEALIKGAEVVLISGPNSLERIPYVNYIEVETAEEMLEKLLEHRGADYLFMASAIEDLVPDRVEGKIKKKDGVSSINIKYAPDVVKSFREKSPGTTVVGFSVEVDNAEENTRKKLEEKGLDFIAWNSPDAFASDFNDIVLYSRSGKRWHLPKGKKNDVAQRIIDIVVQGG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must be able to metal ion binding, phosphopantothenate--cysteine ligase activity.2. A protein that can modulate the overall flux of metabolites through the Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed.3. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A.4. The presence of DNA/pantothenate metabolism flavoprotein C-terminal domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MTVIVGITGGIAAYKAVGVVRDLVKRGHDVHVVPTEGALRFVGLPTLEALSRNPVTTSVWDDVAEVRHVALGRRADLVVIAPATADSLARMAHGLASDLLGTTLLATEAPVVVAPAMHPQMWEHPATRANVATLRDRGVHLVGPVVGALTGDDAGIGRMAEPEDIVAAALDLASALLAGRHLVVTAGGTREPLDPVRFLGNRSSGKQGYAFARAAVARGARVTLISANVSLAAPAGVDLVRVGSTGELRTATLAAAEDADALVMAAAPADFRPAHYATGKIKKSDDGGAPTIELVTNPDIAAEWGRLRRPGQVLVVFAAETGDAEANGRAKLARKRADLVVVNEVGVDKVFGAETNAAAVLGADGEVVALAGRHLVVTAGGTREPLDPVRFLGNRSSGKQGYAFARAAVARGARVTLISANVSLAAPAGVDLVRVGSTGELRTATLAAAEDADALVMAAAPADFRPAHYATGKIKKSDDGGAPTIELVTNPDIAAEWGRLRRPGQVLVVFAAETGDAEANGRAKLARKRADLVVVNEVGVDKVFGAETNAAAVLSADGEVVAEAAGSKRAVGERVLDAVRA",
    "response#2": "MTVIVGITGGIAAYKAVGVVRDLVKRGHDVHVVPTEGALRFVGLPTLEALSRNPVTTSVWDDVAEVRHVALGRRADLVVIAPATADSLARMAHGLASDLLGTTLLATEAPVVVAPAMHPQMWEHPATRANVATLRDRGVHLVGPVVGALTGDDAGIGRMAEPEDIVAAALALLDPAATPRPLAGRHLVVTAGGTREPLDPVRFLGNRSSGKQGYAFARAAVARGARVTLISANVSLAAPAGVDLVRVGSTGELRTATLAAAEDADALVMAAAPADFRPAHYATGKIKKSDDGGAPTIELVTNPDIAAEWGRLRRPGQVLVVFAAETGDAEANGRAKLARKRADLVVVNEVGVDKVFGAETNAAAVLRPGGEEPRLSGRRVVVSAGGTRERLDPVRFLGNRSSGRQGYALARAALARGAEVTLVAANVSLADPAGVKVVHVESTAELRDAVLSAAATADAVVMAAAPADFRPESTSDTKIKKADDGSAPSITLQQNPDILKEIAHDRARPDAVIVGFAAETGDATGSVLDLARAKLARKGCDLLVVNDVSGGAVFGSTDNEAVILGADGSETPVPYGPKSALAHTVWDLVAARLPA",
    "response#3": "MTVIVGITGGIAAYKAVGVVRDLVKRGHDVHVVPTEGALRFVGLPTLEALSRNPVTTSVWDDVAEVRHVALGRRADLVVIAPATADSLARMAHGLASDLLGTTLLATEAPVVVAPAMHPQMWEHPATRANVATLRDRGVHLVGPVVGALTGDDAGIGRMAEPEDIVAAALAALRDGPLAGRRLVVTAGGTREPIDPVRFLGNRSSGRQGLAIASAAAHAGGSVQVIAANIESALLSALPAGVQVTRVGSALQMRDATIAAVARADALVMTAAVADFRPEVASVSKMKKDPTNEDVPTIRLIRNPDILAEIGHSEQRPPLVVGFAAETGTDEEILAYGADKAARKGADFICLNRVGESVGFGDVPNDIRVLTAEGEVVAEPKGTKADLADAVWDLVVPLLR"
  },
  {
    "reference": "MADLVQNAANNVLNDGMDTSRHTSSTAAPPSRNEVELNGQPPTAPPPQVVTDSEEDEDEELTLKYGAKHVIMLFIPVTLCMVVVVATIKSVSFYTQKDGQQLIYTPFREDTETVGQRALHSMLNAIIMISVIVVMTLVLVVLYKYRCYKVIQAWLFFSNLLLLFFFSLIYLGEVFKTYNVAMDYFTLALIIWNFGVVGMICIHWKGPLRLQQAYLIMISALMALVFIKYLPEWTAWLILAAISVYDLLAVLCPKGPLRILVETAQERNEAIFPALIYSSTMVWLFNMADSAETRNNSSHPVPQQENQVVAMAPTAQAEDDGGFTPAWVDHQQHQLGPMQSTEESRRQIQEMPSARPPPPADDDEERGVKLGLGDFIFYSMLVGKASATASGDWNTTLACFVAILIGLCLTLLLLAIFKKALPALPISITFGLVFYFATDNLVRPFMDQLAVHQFYI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Requires the presence of the other members of the gamma-secretase complex for protease activity; Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid- beta precursor protein)2. A protein with beta-catenin binding functions is required to enable lymphangiogenesis, amyloid-beta formation, melanocyte differentiation.3. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "ACYRTFNKTHEFGCQANRENENGLIVRIDKQEDFKNLDSCWNSFYPKYSGKYWALLPVNLIRRDTISQLKSSKCLSGIVLYNSGESIHPGDESTAASHDAECPNAASDYYLQDKNEEYCERKINSRGAITRDGLMKIDWRIQMVFIDNSTDLEIIEKCYSMFNKPKEDGSSGYPYCGMSFRLANMA",
    "response#2": "ACYRTFNKTHEFGCQANRENENGLIVRIDKQEDFKNLDSCWNSFYPKYSGKYWALLPVNLIRRDTISQLKSSKCLSGIVLYNSGESIHPGDESTAASHDAECPNAASDYYLQDKNEEYCERKINSRGAITRDGLMKIDWRIQMVFIDNSTDLEIIEKCYSMFNKPKEDGSSGYPYCGMSFRLANMC",
    "response#3": "ACYRTFNKTHEFGCQANRENENGLIVRIDKQEDFKNLDSCWNSFYPKYSGKYWALLPVNLIRRDTISQLKSSKCLSGIVLYNSGESIHPGDESTAASHDAECPNAASDYYLQDKNEEYCERKINSRGAITRDGLMKIDWRIQMVFIDNSTDLEIIEKCYSMFNKPKEDGSSGYPYCGMSFRLANMA"
  },
  {
    "reference": "MDILTAAEMREMDRETIQSFGLPGRVLMENAGRGVFQLLMETFPEAVQGGVAVAAGRGNNGGDGFVVARYLATYGIPVTVYLLADSSRLQGDAADNFNLLAPLDVRVRELPDRDAFSRVQNEMAGHALWIDAILGTGLSAEVTGYFKSVIEFINDLDRPVLAVDLPSGVHSDTGAICGICIRAAATVTFAYPKIGHCIHPGAAVCGNIHVIDIGIPNPIAARIGTRQHLLTPSSIKALWRPRPHDAHKGRTGHLLAIAGGIGKTGAAALCALSGLRSGAGLVTLAVPETLQPMAAAHALEIMTAPIPDNGDGLFSDAGFKSIMTLIEDKRVLALGPGIGTAPQTRDLITRLIPQCLIPMVIDADGINIIAEDPDILLKRRADMVLTPHPGEMARLVNTTPAAIQSDRIGHARQFAEKYKVCLVLKGARTVIARPDGHVYINSTGNAGMASAGMGDVLTGMIAGFMAQGYAPDKAARLAVYLHGDAADAMAREKGPIGYIAGDVMAAVPAAMGRIMATETETAARDILSARLFS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein have enhanced (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.2. The protein must exhibit NADHX epimerase activity.3. The protein should be able to bind K(+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MDQHKGQGGHTVVMGGSIPYLGAATMAGLGAVASGAALVTLSVHSSLAKEARMGYPGLMVASGNPEAPGWPRGKVASLVIGPGWGDEGLAYWFAVVMGDGPWCGLPTVFDADALNILAHDPGLRACDLPLNPSILTPHTGEAARLLAMSAEQVLENRVAAARVLAEKHRAVVVLKGPSTLIAAPDGRLAVNPTGHPLLAQGGTGDVLAGIIGGLQAQGMASFEAACCGTFLHGWAAERLAEHQGESGLRPELLA",
    "response#2": "MDQHKGQGGHTVVMGGSIPYLGAATMAGLGAVASGAALVTLSVHSSLAKEARMGYPGLMVASGNPEAPGWPRGKVASLVIGPGWGDEGLAYWFAVVMGDGPWCGLPTVFDADALNILAHDPGLRACDLPLNPSILTPHTGEAARLLAMSAEQVLENRVAAARVLAEKHRAVVVLKGPSTLIAAPDGRLAVNPTGHPLLAQGGTGDVLAGIIGGLQAQGMASFEAACCGTFLHGWAAERLAEHQGESGLRPELLNL",
    "response#3": "LTQAQIAQWLPRRTMDQHKGQGGHTVVMGGSIPYLGAATMAGLGAVASGAALVTLSVHSSLAKEARMGYPGLMVASGNPEAPGWPRGKVASLVIGPGWGDEGLAYWFAVVMGDGPWCGLPTVFDADALNILAHDPGLRACDLPLNPSILTPHTGEAARLLAMSAEQVLENRVAAARVLAEKHRAVVVLKGPSTLIAAPDGRLAVNPTGHPLLAQGGTGDVLAGIIGGLQAQGMASFEAACCGTFLHGWAAERLAEHQGESGLRPELLAA"
  },
  {
    "reference": "MGKDVALDFLPSSLYSCTCSCDGKLAFCDSEKVQILVPSTEANTPKFMKALAYLDDVEDELPNCQMLAPNELRLGDLTPSVISRYVVWSPTGLADNYDPLLTVLTNRHRVYFFSSSSRVLHQKWTCVAQMSTHLPSDPLELCRIHSVAWSPLVSLPSSSPWGVCLFALGAESGHVHLSIMSHSSTPFFKSFDLNCSWVVQLSFSSWNVVGDSATCLLSCSSRNGEIRILKIAISSHSDTFDTAMQELPFSLDYRPFSPLLAWSSPLANVEYLALVYPGHLFAFRYDKSLGKFCSFLNHNLLSLCSPSGVLFGHNDIDTIYVYILTHSGTLETFSLLDNSISMLDSPERHVLEKFLNNHLQNYGSVSDDSIKTLKIHGFCPSPYLSSAALHFSISYPASFTYVVTAAERSYFNFIPSLFSKSVFSHMITSSLNNLCVAPSAGCLLEISLMNDTLKEKTDIFTMLTNSLSSFLVTDYDFFLELKHLIDISSLSNLYLDSSLNAMRLLYGWSVYKQKALDATLLNSLRYRLTLYIMLYTLSQISLDPSYLTSDCKAVLRNFVSFTYKELAEVPIAMEVANEIAKSLEVSSDFSETCPACEATVQFNNTSLATCDNGHVWRRCSVTMLLLSQKAAKYCAVCNSIVAIFNPSQTKCLLADLQNELSICFYCGGHFLVS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have RNA polymerase III general transcription initiation factor activity, histone acetyltransferase activity within cytosol, transcription factor TFIIIC complex, nucleus to regulate transcription initiation at RNA polymerase III promoter. The designed protein sequence is ",
    "response#1": "DKMVEFLEEKLSCRFPYPTLKVVFVDQCTEEIQVYSSLLIVPTAMLYHKKIIDVVQEARQKLIFSIALQFFGCLISPAQWWHWWIPVSIARFLTSLYVETKLGTAEARWQLKRAMDDVCDYEHQWGKIVLSPPEPTKKLPLHVDPRHEYTASPLYVEAMLKKGFLTMRMLQRRIGLEPFMRVLHRVLTG",
    "response#2": "MLNHRKFRTGTKSEIDDLLRVEKGVNPKMVVYSFGVSHEHPGSFILSYIRSANPHHEYIGLYPKGFKFRKRMFGDLDKLAAYFKRHR",
    "response#3": "LPLLDPSLYISRFASLLEFGPETHRVANDAIRLVARMNRDWMQIGRRPAGICGACLLIAARMNNFRRSVREVVHVVKVADITIQKRLDEFD"
  },
  {
    "reference": "MGGKWSKMAGWSAVRDRMRRAEPAADGVGPASRDLEKHGALTTSNTAENNAACAWLEAQEEEEVGFPVRPQVPLRPMTYGAALDLSHFLKEKGGLDGLVYSQKRQDILDLWVYHAQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPAEPEEVEKANEGENNSLLHPISLHGMEDTEKEVLVWRFDSRLAFHHVAREQYPEYYKDC",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike2. The designed protein should have GTP binding within host cell plasma membrane, membrane to regulate suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host autophagy.3. Incorporating a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Dileucine internalization motif; necessary for CD4 internalization motif is critical for the successful design of the protein. The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPAIRERMRRTEPAAEGVGAASQDLEKHGALTSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYNTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPEKVEEANKGENNSLLHPMSQHGMDDPEREVLEWRFDSRLAFHHVARELHPEYYKN",
    "response#2": "MGGKWSKSSIVGWPAVRDRIRRTEPAAEGVGAASRDLDKYGALTSSNTAHNNAACAWLEAQEEAEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLVYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPEKVEEATEGENNCLLHPINQHGMDDPEREVLVWKFDSRLAFHHKARELHPEYYKDC",
    "response#3": "MGGKWSKSSLVGWPAVRERMRRAEPAADGVGAASRDLEKHGALTSSNTAANNADCAWLEAQEGEEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGTRYPLTFGWPFKLVPVDPDQVEEANEGENNCLLHPMSQHGMEDEEREVLKWKFDSSLARRHLAREKHPEFYKDC"
  },
  {
    "reference": "MRVKGIGKNYHYVWRWGTMLLGMLMICNAEEKWWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEIELKNVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDVNNNATALNKTTNPLKISEGKGEIKNCSFNITTSIRDKVQKEYALFYKLDVVPIDNDNTSYRLISCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGQCNKVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVIRSENFTDNAKIIIVQLNKSVEINCTRPNNNTRKSIHIGPGRAFYATGEIIGNIRQAHCNLSRAEWNDTLKQIAIKLREKFGNNKTIVFNQSSGGDPEIVMHSFNCGGEFFYCNSAQLFNSTWEANNTWENDNSTEGNIMLPCRIKQIINMWQEVGKAMYAPPIRGQIRCSTNITGLLLTRDGGNNGTNETEIFRPGGGNMKDNWRSELYKYKVVKIEPLGVAPTKARRRVVQREKRAVTLGALFLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTAVPWNTSWSNNKSLNDIWNGMTWMQWEKEIDNYTDLIYTLIEQSQNQQEKNEQELLELDKWASLWNWFDISKWLWYIKIFIMIVGGLVGLRIAFSVLSIVNRVRQGYSPLSFQTRLPAQRGPDRPEGIEEEGGERDRDRSGPLVDGFLALIWVDLRSLCLFSY",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must contain a signal peptide for proper functionality.2. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.3. The designed protein have Human immunodeficiency virus 1 envelope glycoprotein Gp120, Retroviral envelope protein GP41-like domains with specific sequence motifs that are crucial for its activity.4. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells. The designed protein sequence is ",
    "response#1": "MGAASVTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKSISEIWENMTWMQWDKEINNYTNTIYRLLEESQTQQEKNEQDLLALDSWKNLWSWFDISRWLWYIKIFIMIIGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTN",
    "response#2": "MGAASVTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKSISEIWENMTWMQWDKEINNYTNTIYRLLEESQTQQEKNEQDLLALDSWKNLWSWFDISRWLWYIKIFIMIIGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTR",
    "response#3": "MGAASVTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKSISEIWENMTWMQWDKEINNYTNTIYRLLEESQTQQEKNEQDLLALDSWKNLWSWFDISRWLWYIKIFIMIIGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTL"
  },
  {
    "reference": "MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPPNVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGITRYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVDLYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTRPETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPAHDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKIEDHFHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFNQWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSSALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVLLHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVESVWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDKYEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHPFMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEVNTPLSKEIPILIQAKDKEIETTLSQNKD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a high affinity for ATP.2. The protein design must feature the 'HIGH' region, 'KMSKS' region motif.3. The protein must exhibit the following characteristics: Catalyzes the attachment of valine to tRNA(Val); As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner..4. A protein that contributes to valyl-tRNA aminoacylation by functioning in cytoplasm with ATP binding, valine-tRNA ligase activity, aminoacyl-tRNA editing activity.5. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "LDEKQMKIGRRLAIKILNASKFALSVAGEDTVADPAAVTEPLDRSMLAVLAEVVQDATAAFHEYDHTRALERTERFFWNLCDDYLELVKARAYDPESREGASARAALLIALSVLHRLFAPFLK",
    "response#2": "WDMKRLEGYRNFCNKLWNASRYVMMNTEEQDCGFGAGEIEYSLADKWIESQFELAAKAFNNHIDNYRLDMAANTLYEFIWNQFCDWYLELTKPVLWKGTEAQQRGTRRTLITVLEKTLRLAHPVIPYITETIWQSL",
    "response#3": "LDEKQMKIGRRLAIKILNASKFALSVAGEDTVADPAAVTEPLDRSMLAVLAEVVQDATAAFHEYDHTRALERTERFFWNLCDDYLELVKARAYDPESREGASARAALLIALSVLHRLFAPFLP"
  },
  {
    "reference": "MELVVATGNAGKLREIRRRLEGIDIEVLGMDVFVDLPEVIEDGDTFAENAAKKAQTIAAATGRMTLADDSGLEVAVLDGRPGVHSARYAGEDADDAANNAKLLRELSAVPPERRQGAFVCVMALCLPEGECRYFSGRLEGEILSSPRGDGGFGYDPLFWLADKQKSLAEIPLDEKNGISHRGQALDQVVKVLQGD",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for dITP + H2O = dIMP + diphosphate + H(+).2. The protein design must be able to perform ITP diphosphatase activity, XTP diphosphatase activity, dITP diphosphatase activity in order to contribute to purine nucleoside triphosphate catabolic process, nucleotide metabolic process.3. The Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.4. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..5. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity. The designed protein sequence is ",
    "response#1": "MTITTPQPSTSTGGTRGR",
    "response#2": "MFTPIVLASGSKRRRQHLLAAGVVPDRLMPADIDEDASLPERAPALVAANARGKAAAAALPGAWVIGSDQVAELDGQLLGKPGTAEKARTQLMRLRGRTATFWTGVCVIRPDERIVESVVTRTRVVFRELTDAEIERYVAAGEPFDCAGSFKSEGLGIALIERIDGDASSVVGLPLVELTRMLRRFGLDPWGCG",
    "response#3": "MSGATLILASGNAGKVRELSALLAPMGIAVRPQSEFDVSEVPETGLTFVENALLKARHAALHTGLPALADDSGLAVDALGGAPGIYSARFAGEGASDAENNAKLLAELGGVPAERRTAHFVCVLVLVRHADDPLPIVCEGLWHGRILDEPRGDGGFGYDPLFLVPALQCTAAELPSGAKNALSHRGRALAALIERLRDRPG"
  },
  {
    "reference": "MTVRTRVAPSPTGDPHVGTAYIALFNLCFARKHGGQFILRIEDTDQSRSTPESEQAILDSLRWLGLEWDEGPDVGGAAGPYRQSERKEIYAQYVQQLLDAGHAFKCYRTTEELDMLRAARKEAGIHSALKQSDLMLTEQEQAEREAAGIPYVVRMKVPEEEGACQVTDLLRGNIDLDWSMVDAQILMKSDGMPTYHLANVVDDHLMKITHVIRGEEWINSAPKHILLYQYFGWDVPVFCHLPLLRNPDKTKLSKRKNPTSILYYKQAGYLPEALTNYLGRMGWSMPDESEKFSIQEMLNHFDIARVSLGGPVFDIEKLNWLNGLWIREDLDDAALAQRLVDWKFNQDNLLAVIPHVKQRMETLGDFLPMVSFLAANTLGITEESFKGNKLDLEDQKKVLQFALWQLDTMRTWERDDIFATLKGLADGMGIKLKDFLAPLFVAISGSTASFSVMDAMVLLGSDLSRARLRVAVEVLGGAGKKVLKRYEKEFAALSQLNEESDAE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must be able to tRNA binding, glutamate-tRNA ligase activity, ATP binding. The designed protein sequence is ",
    "response#1": "DHRKLGRQLDFFHMQDEAPGMVFWHPRGWVIWQEVEQYMRNMFREFGYQEVRTPTIMDRVMWEKSGHWQNYHDNMFTTASENRDYAVKPMNCPGHIQIFNSTLHSYRDLPLRLAEFGSCHRNEPSGALHGLMRVRGFTQDDAHIFCTEDQVKDEVADFIVMLYKAYKDFGFNEVLVKLSTRPEKRVGTDEAWDKAEEALAVALRQNNLDFELQPGEGAFYGPKIEFTLKDSLGRLWQCGTIQLDFNLPERLGAEYVDEDNSRKHPVMLHRAIVGSMERFLGILIENYAGAMD",
    "response#2": "FTTHSENRDYAVKPMNCPCHIQVYNQGLKSYRDLPFRMAEFGSCHRNEPSGALHGIMRVRNFVQDDGHIFVTEGQIQQEVSEFIDLLHEVYADFGFDNIVYRLSTRPEQRVGSDEVWDKAEKALSEALDSAGLDWEELPGEGAFYGPKIEFSLKDAIGRVWQCGTIQVDFSMPTRLGAQYVSEDGSRQTPVMLHRAIVGSLERFIGILIEW",
    "response#3": "WQKVGLTPANPDAQHLVATIELLKPRARNLKDFAGSFKAFFTDDYANDPEAVEKFLKDPAVREMLVELGERYANAGEFTEQSTEQVLRDLAAEKGVKAGGLINGARVALTGQAVAPSLFAVMVNLGREKTVSRLRRR"
  },
  {
    "reference": "MTEQAQKTTHFGFQTVAEEEKAGMVHGVFTRVASRYDLMNDLMSAGIHRVWKDAMMDWLAPQPHQRLLDVAGGTGDISFRFLRRAPGATAVVCDMTESMLVEGRKRAEAESMGESLSWVVGDGMALPFADNSFDVVTNAFGTRNITRIEDSLREGFRVLKPGGRMMTLEFSQMPNPMLQWAYDRYSFNVIPVMGQFVAGDRDSYQYLVESIRKFPDQETFAQMFRDAGFGQVKYRNLSLGIAALHSGWKI",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein have enhanced a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine.2. We require a protein that can specifically bind to the metabolites produced in the menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2, Quinol/quinone metabolism pathway.3. The designed protein must possess S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity, demethylmenaquinone methyltransferase activity, 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity to support ubiquinone biosynthetic process, aerobic respiration, menaquinone biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "MSSFQFGFKEVPAAEKTHRVAQVFSSVASRYDLMNDLMSFGLHKSWKKFTLELSGVRAGDKVLDIAGGTGDLSALFAPRVGKQGQVVLADINDSMLRVGRDRLIDRGIAGNIDYAQADAESLPFPDNSFDCITIAFGLRNVTDKDAALRSMWRVLKPGGCVIVLEFSKPTAPLGKAYDWYSFKVLPWLGQKVAGDAESYRYLAESIRMHPDQETLKGMMEQAGFDRVDYWNLTAGVVALHRGYKF",
    "response#2": "MSDQQTNHFGFKTVSESEKQGKVADVFHSVAAKYDIMNDAMSGGMHRLWKRFTIELSGVRAGHRVLDIAGGTGDLAAKFSKLVGSTGEVVLADINESMLNVGRDKLTNRGVTGNVDYVQADAQYLPFPDKSFDCITIAFGLRNVTDKDAALRSMQRVLKPGGCVIVLEFSKPTNPLMGKAYDTYSFQVLPKLGQLVAGDSESYQYLAESIRMHPDQETLKTMMTQAGLDRVEYFNLTGGIVALHRGFKF",
    "response#3": "MSIYDVMNDLMSFGIHRVGKMATALAPELAADIKTITHEMTED"
  },
  {
    "reference": "MGYKVAVAGATGAVGREILRILAERNFPADEVVALASSRSVGREVSFGEDKVLKVQNLETFDFKGCDIALFSPGAEISAKYAPKAARERCVVVDNTSHFRMEPGVPLVVPEVNADAVYKARRGIIANPNCSTAQLVVALKPLHELFRIKRVVVSTYQAVSGAGKDGMDELFAQTKGSFVHDSPDIAQFTKQIAFNVIPHIDRFLEDGSTKEEWKMAVETRKILDPDIRVLATCVRVPVFIGHSEAVSIECEKPVDLEKAWEALRRAPGVVVQDKREDGGYVTPIESVGEDPVYVSRLRVDPSVEHGLAFWCVADNLRKGAALNTVQIAECLAERNLLGPHSELWKKHERPLEPQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should consider the endogenous proteins and enzymes present in the L-threonine biosynthesis, L-threonine from L-aspartate: step 2/5, Amino-acid biosynthesis pathway to avoid interference with native metabolic processes.2. The protein's Acyl-thioester intermediate, Proton acceptor must be carefully engineered to ensure that the amino acids involved in L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH are properly positioned for optimal catalysis.3. The protein design must be able to perform protein dimerization activity, aspartate-semialdehyde dehydrogenase activity, NADP binding in order to contribute to lysine biosynthetic process via diaminopimelate, 'de novo' L-methionine biosynthetic process, isoleucine biosynthetic process, diaminopimelate biosynthetic process.4. The designed protein should be able to compete with endogenous proteins or inhibitors for phosphate, NADP(+) binding.5. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate. The designed protein sequence is ",
    "response#1": "MTTLAVVGATGQVGAVMRRLLEERDFPATTVRFFSSARSAGTTLPWKGGEVTVEDVATADLTGIHIALFSAGATASREHAPRFAAAGAVVVDNSSAWRMDPDVPLVVSEVNPVAAATRPKGIIANPNCTTAAMVVALNPLHKRYTIKRVVVSTYQSVTGTGVKAVEQMMNERKGITDGPMAYHYPIDQNAIPHIDAFLENGYTKEEMKMVNETKKIMGDNSIAVTATCVRIPTLGGHSESVNIEFEKDFDLQELKNLLAASPGLVVKDDVANAIYPMPLTANEKDDTFVGRLRRDETRPNTLNCWIVSDNLRKGAALNTAQIAEVVAAELA",
    "response#2": "MTKTGLVGWRGMVGSVLMGRMRDENDFANIEPTFYSTSNAGGEAPDVGKGAGVLQDAENIDSLKEMDVIISCQGGDYTKAVFPKLREVGWDGYWIDAASALRMSDDAVIILDPVNGDVISDALARGAKDFIGGNCTVSLMLMALNPLHKRYTIKRVVVSTYQSVTGTGVKAVEQMMNERKGITDGPMAYHYPIDQNAIPHIDAFLENGYTKEEMKMVNETKKIMGDNSIAVTATCVRIPTLGGHSESVNIEFEKDFDLQELKNLLAASPGLVVKDDVANAIYPMPLTANEKDDTFVGRLRRDETRPNTLNCWIVSDNLRWGAAEPLRRILRILTERT",
    "response#3": "MTKTGLVGWRGMVGSVLMGRMRDENDFANIEPTFYSTSNAGGEAPDVGKGAGVLQDAENIDSLKEMDVIISCQGGDYTKAVFPKLREVGWDGYWIDAASALRMSDDAVIILDPVNGDVISDALAKGGKNWIGGNCTISCMLMGVGLNPLHKRYTIKRVVVSTYQSVTGTGVKAVEQMMNERKGITDGPMAYHYPIDQNAIPHIDAFLENGYTKEEMKMVNETKKIMGDNSIAVTATCVRIPTLGGHSESVNIEFEKDFDLQELKNLLAASPGLVVKDDVANAIYPMPLTANEKDDTFVGRLRRDETRPNTLNCWIVSDNLRWGAAEPLRRMLKILVE"
  },
  {
    "reference": "MIPNRWRDLARRDIFEPLGLNGSYFIPNAHNRAHVAVASKYSDEVDIDFLDVMACSGGQMSSLSDYIKLMQTFLDPTLPQSLLPAHIMREWMTPVFGFNDDETEVGLLWEIVKIQDSYCRPVRVYEKNGVLGASRSVFAIHREMAFGVALLNTGTATVTGNIALEIFRIMQPYLDKLQERKVKERFAGHWRLPLQTNATSGSMVDISVSDGSLWITRLVLNGTDVLSLTEAMPAFGGARSRRVALWSMRRDEFRMVLGAGAATSCMSSWTAMDSGFSRGYPMDLVYFKGGRLHIPSAGVVLVRA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that It is possible that unknown proteins encoded in, or near, the cluster (such as str1 or stl1) may form new classes of halogenases or dimethylally-transferases, or that the responsible genes are located elsewhere on the genome (Probable); The short-chain dehydrogenase stl2 and the dehydrogenase str4 play a role in the shunt pathway leading to the production of bolineol; Str9 performs epoxidation of the 2,3 olefin of prestrobilutin A, followed by Meinwald rearrangement to furnish the aldehyde intermediate (Probable); The cluster encodes no obvious halogenase gene that could be involved in production of strobilurin B, nor any obvious dimethylallyl-transferase that could be involved in the production of strobilurin G (Probable) The designed protein sequence is ",
    "response#1": "MPFYSESSELAVKMKRSRKGPLNYDTLAVNALTHPDLAEHVIKGEPILPATGFFEMIFEEGARTIWDIELRSLLPLLPEKVLNVNVKSDGHAWSIVSSSGGRNPRLHATGFMTTEVMDKDAGPIDLAAIRARTTPADISNLYAILNNTAAFGPLYRRIEACYEGDHEILYQVRGNAPELTAHYNYVFHPSLLDSCIHGLLHPVFTGNADKSVFYLPSHIGRVTLYDRAIEEAVPETLYSYVVPHDWTPDSIACDAFIVNERGERLVTLIDCVLSKHWTGAVPAGGR",
    "response#2": "PKPVAVVGISAEFPSGTLSDANFDHQSFFDFLLSGKDAVERIPKDRFNIDGWQGSHLGQILPEDACFLKNVHLFDHFEFGISSKDALTMGAGTRKLVEHSFLALLDSGINCRSQNVAAFSSAVAFDLLSAADADEFEPRDGFGGGAAAVANRISYQLDLLGPSIPVDTACSSSLMALHLGVQSLRAGECEAAVIGGSQINHRFLDWIFYSQLSILSPGGKSIPFDSSADGFGRGEAVVVLVVKLLEDAIRDGDKIYATVLNTAVNSTGSAGPVKTPIAESQAAAMLTAYKGIGRSPSEADFIECHATGTSVGDPVEANWVGNHFKRDSELLIGSVKGNVGHTEITSFLTSFSKVISMFDTNRIPPQANFKEPNPAIHWEEYNMRTPTQIEGFTTRNPSGKRLASINASGLLGANGHVIAEVAVVGISAEFPSGTLSDANFDHQSFFDFLLSGKDAVERIPKDRFNIDGWQGSHLGQILPEDACFLKNVHLFDHFEFGISSKDALTMGAGTRKLVEHSFLALLDSGINCRSQNVAAFSSAVAFDLLSAADADEFEPRDGFGGGAAAVANRISYQLDLLGPSIPVDTACSSSLMALHLGVQSLRAGECEAAVIGGSQINHRFLDWIFYSQLSILSPGGKSIPFDSSADGFGRGEAVVVLVVKLLEDAIRDGDKIYATVLNTAVNSTGSAGPVKTPIAESQAAAMLTAYKGIGRSPSEADFIECHATGTSVGDPVEANWVGNHFKRDSELLIGSVKGNVGHTEITSFLTSFSKVISMFDTNRIPPQANFKEPNPAIHWEEYNMRTPTQIEGFTTRNPSGKRLASINASGLLGANGHVIAESPP",
    "response#3": "PKPVAVVGISAEFPSGTLSDANFDHQSFFDFLLSGKDAVERIPKDRFNIDGWQGSHLGQILPEDACFLKNVHLFDHFEFGISSKDALTMGAGTRKLVEHSFLALLDSGINCRSQNVAAFSSAVAFDLLSAADADEFEPRDGFGGGAAAVANRISYQLDLLGPSIPVDTACSSSLMALHLGVQSLRAGECEAAVIGGSQINHRFLDWIFYSQLSILSPGGKSIPFDSSADGFGRGEAVVVLVVKLLEDAIRDGDKIYATVLNTAVNSTGSAGPVKTPIAESQAAAMLTAYKGIGRSPSEADFIECHATGTSVGDPVEANWVGNHFKRDSELLIGSVKGNVGHTEITSFLTSFSKVISMFDTNRIPPQANFKEPNPAIHWEEYNMRTPTQIEGFTTRNPSGKRLASINASGLLGANGHVIAELSYGLDSLSAARMSTALVAVVGISAEFPSGTLSDANFDHQSFFDFLLSGKDAVERIPKDRFNIDGWQGSHLGQILPEDACFLKNVHLFDHFEFGISSKDALTMGAGTRKLVEHSFLALLDSGINCRSQNVAAFSSAVAFDLLSAADADEFEPRDGFGGGAAAVANRISYQLDLLGPSIPVDTACSSSLMALHLGVQSLRAGECEAAVIGGSQINHRFLDWIFYSQLSILSPGGKSIPFDSSADGFGRGEAVVVLVVKLLEDAIRDGDKIYATVLNTAVNSTGSAGPVKTPIAESQAAAMLTAYKGIGRSPSEADFIECHATGTSVGDPVEANWVGNHFKRDSELLIGSVKGNVGHTEITSFLTSFSKVISMFDTNRIPPQANFKEPNPAIHWEEYNMRTPTQIEGFTTRNPSGKRLASINASGLLGANGHVIAESPP"
  },
  {
    "reference": "MEKENVFASVEEALEDIRAGKMVIVVDDEDRENEGDFIVAAEFATPEIINFMAKEGRGLICLALPEDDFRRLNLEIPLTNTSKHGTNFGIPIDAKSGTTTGSSAYDRSRTILLAIDPLTGPEDFARPGHVYTLKALNGGVLRRAGHTEAAVDLARLAGLRPAGVLCEIMDDSGEMARLPKLIEISKKFGFKIISIRSLINYRVKREKLIEKITEVNLPTKWGFFRLHAYLDKLTQEVHLALVKGDVRGKENVLVRVHSQCITGDIFGSLRCDCGDQLHKAMEMIDKEGLGVFLYMRQEGRGIGLVNKLKAYHYQDLGYDTVEANEKLGFPPDLRDYGIGAQILVDLGLSTIRLLTNNPRKIVGLEGYGLKVVERIPIVTKPNPENLRYLTVKRDKLGHLIGPIEKDLEEQK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..2. The Zn(2+), D-ribulose 5-phosphate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "REVSVQMPTEYGDFHLVAYKQLTNDQNHLALVKGSWEPEEPVLVRVHSSCITGDIFGSCRCDCGPQLHTALQMIEQEGKGVLVYMNQEGRGIGLINKLRAYKLQEEGLDTVEANIKLGFNMDHRDYGIGAQILRDLGITKMKLLSNNPKKRTGLIGYGLEVVDNVRAATITLPTRFGEFTAHAYHSLVDDKPYLALTLGDVSQDGTLVRVHSSCLTGDVFHSLRCDCGAQVETALKMIQRETRGVLLYIQQEGRDIGLLNKIRAYELQEHGRDTVDANRELGFPDDPREYGIGYQILADLGVRRMRILTNNPRKWVNIEAYGLEVMERVA",
    "response#2": "RMSEAELPTKFGTFKVVGYVDQIDGAEHVALVKGDVAGKKNVLVRVHSECLTGDVFGSYRCDCGSQLQRALELIGEQGEGVLVYMRQEGRGIGLKNKIDAYHLQDQGMDTVEANEHLGFDADLRDYGTAAQILCDLGLTSIHLITNNPKKIIGISGYGLTIAKQIG",
    "response#3": "RMSEAELPTKFGTFKVVGYVDQIDGAEHVALVKGDVAGKKNVLVRVHSECLTGDVFGSYRCDCGSQLQRALELIGEQGEGVLVYMRQEGRGIGLKNKIDAYHLQDQGMDTVEANEHLGFDADLRDYGTAAQILCDLGLTSIHLITNNPKKIIGISGYGLTIAKQIG"
  },
  {
    "reference": "MKVKQHTIKKQVTLNGVGLHTGIVANMTFLPASPNHGYKFQRMDLEGQPIIDADVDNVVDVSRGTTLEQGGARVFTVEHVLAALVGMEIDNVLIQLNGPEPPILDGSSIQFINILEDAGLEEQNALRRFFEVPESIHYKDSAREVEMAALPLDDFRVTVMVDYNSPVLGSQHASITDISQFRHEIASCRTFCFLHELEMLYKNNLIQGGDLNNAIVVVDRIVEEAELANLAKMFNKQKVEVKKEGILNNVELRYKNEPARHKLLDVIGDLALVGRPLKAQILAARPGHAANVAFAKKIKRAMEKAGPMHIPYYDPKSPPVLDINQISNILPHRYPFQLLDKVIYLDDKVVAGVKNVTINEPFFMGHFPNNPVMPGVLQVEAMAQTGGILVLSTVEDPENYWTYFLSIENCKFRKMVLPGDTLIFKCELLNPIRRGIAKMKGEAFVGNALVCEAIMTASIVRKDA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be involved in lipid A biosynthetic process by localizing to cytoplasm and exhibiting UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity, UDP-3-O-acyl-N-acetylglucosamine deacetylase activity, 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity.2. The protein's Proton donor must be carefully engineered to ensure that the amino acids involved in a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine +   H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate are properly positioned for optimal catalysis.3. The protein design should take into account the physiological conditions of the lipid IV(A) biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MAYDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPTMHAIRHVYKLIYRSGKTIEEVLPEIEHIALNEPAIKVYLDFFKHSTRGIDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPTMHAIRHVYKLIYRSGKTIEEVLPEIEHIALNEPAIKVYLDFFKHSTRGIDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPTMHAIRHVYKLIYRSGKTIEEVLPEIEHIALNEPAIKVYLDFFKHSTRGIDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPTMHAIRHVYKLIYRSGKTIEEVLPEIEHIALNEPAIKVYLDFFKHSTRGIDSGFKEDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPTMHAIRHVYKLIYRSGKTIEEVLPEIEHIALNEPAIKVYLDFFKHSTRGIDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPTMHAIRHVYKLIYRSGKTIEEVLPEIEHIALNEPAIKVYLDFFKHSTRGIDSGFKESDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPTMHAIRHVYKLIYRSGKTIEEVLPEIEHIALNEPAIKVYLDFFKHSTRGIDSGFKER",
    "response#2": "MTDDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPTMHAIRHVYKLIYRSGKTIEEVLPEIEHIALNEPAIKVYLDFFKHSTRGIDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPTMHAIRHVYKLIYRSGKTIEEVLPEIEHIALNEPAIKVYLDFFKHSTRGIDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPTMHAIRHVYKLIYRSGKTIEEVLPEIEHIALNEPAIKVYLDFFKHSTRGIDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPTMHAIRHVYKLIYRSGKTIEEVLPEIEHIALNEPAIKVYLDFFKHSTRGIP",
    "response#3": "MRSDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPTMHAIRHVYKLIYRSGKTIEEVLPEIEHIALNEPAIKVYLDFFKHSTRGIDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPTMHAIRHVYKLIYRSGKTIEEVLPEIEHIALNEPAIKVYLDFFKHSTRGIDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPTMHAIRHVYKLIYRSGKTIEEVLPEIEHIALNEPAIKVYLDFFKHSTRGIEPDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPTMHAIRHVYKLIYRSGKTIEEVLPEIEHIALNEPAIKVYLDFFKHSTRGIEPRE"
  },
  {
    "reference": "MIDLFDDTYAATRDAADPLRDLRSEFLIPRHDGEPMAYFVGNSLGLQPRGARAYVEEVLRKWEEEGVEGHFTGDAQWMTYHDLVREPLARLVGAEPLEVVAMNTLTVNLHLLMASFYRPTAERPVLLIEAGAFPSDRHAAWSQVRFHGFDPRTDLVEVAPDAPDGTISMDAIARAIVAHRDRLALVLWPGVQYRTGQAFDLAEIARLARDAGAACGFDLAHAVGNVDLSLHDSGADFAVWCHYKYVNAGPGAVAGAFVHARHSRADLPRLAGWWGHDPSSRFRMEPEFVPAPGADGWQLSNPPILAMAPLRASLALFERAGMPALRAKSCSLTGYLEQLIDARLRGMLDVLTPRDPVQRGCQLSLRVHGGRDRGRSLFEFLAARGVLGDWREPDVIRISPTPLYNRHADVLRFARAVEAWSTAA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The ideal protein would be one that plays a critical role in tryptophan catabolic process, anthranilate metabolic process, 'de novo' NAD biosynthetic process from tryptophan, quinolinate biosynthetic process, L-kynurenine catabolic process through kynureninase activity, 3-hydroxykynureninase activity within cytoplasm.2. The protein should have a low Km value for H2O + L-kynurenine = anthranilate + H(+) + L-alanine.3. The protein must bind to pyridoxal 5'-phosphate in order to perform its enzymatic function.4. Ideal protein should have a binding affinity for key enzymes in the Cofactor biosynthesis, quinolinate from L-kynurenine: step 2/3, NAD(+) biosynthesis pathway to enhance the overall efficiency of the pathway.5. The presence of Aminotransferase class V domains is critical for the interaction of the designed protein with its intended targets.6. The pyridoxal 5'-phosphate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "LQPKGVEQALRNELKHWREHAVEGHFRGDMPWMHYHKFLQKQSAAIVGALPEEVVVMNTLTTNLHLLMVSFYRPTPERFKIIMEAGAFPSDQYAMETQVRWHGFDPAHAIVEVAPRPGEQTLRTDDIAATIKQHGAQTAVIMFGGVNYYTGQFFDLRAITAAGHAAGAYVGFDLAHAAGNVPLQLHDWGADFACWCSYKYLNSGPGGPSGIFVHEKHL",
    "response#2": "QPKSAREYMSRQMDNWENLAVEGWFEGATPWMFYHKELKALMANIVGASAAEVCPMNTLTVNLHLLMVSFYRPTAKRFKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVAPRAGEATLRTEDILDTIATNGAETALVLFGGINYFTGQWYDMPAITKAGHHAGAVVGFDLAHAAGNVPLQLHNWDIDFACWCSYKYQNGGPGGISGIFVHEKHFAGGAPPQPAIKEQTAKILGALPTEVVIMETLTANLHLLMSSFYRPTKDRWKIIIEGKAFPSDHYAVLSQIANHSLDPSALITIDPPSASSPYLSNEHILSVISRHAATTALVLLPGIQFYSGQFFDIELITRHCQSLGITVGWDLAHAAGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHQPKSAREYMSRQMDNWENLAVEGWFEGATPWMFYHKELKALMANIVGASAAEVCPMNTLTVNLHLLMVSFYRPTAKRFKIIMEAGAFPSDQYAIESQVRFHGYDPKDAIIEVAPRAGEATLRTEDILDTIATNGAETALVLFGGINYFTGQWYDMPAITKAGHHAGAVVGFDLAHAAGNVPLQLHNWDIDFACWCSYKYQNGGPGGISGIFVHEKHFAGGPPPEPAIKEQTAKILGALPTEVVIMETLTANLHLLMSSFYRPTKDRWKIIIEGKAFPSDHYAVLSQIANHSLDPSALITIDPPSASSPYLSNEHILSVISRHAATTALVLLPGIQFYSGQFFDIELITRHCQSLGITVGWDLAHAAGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVH",
    "response#3": "LQPKGVEQALRNELKHWREHAVEGHFRGDMPWMHYHKFLQKQSAAIVGALPEEVVVMNTLTTNLHLLMVSFYRPTPERFKIIMEAGAFPSDQYAMETQVRWHGFDPAHAIVEVAPRPGEQTLRTDDIAATIKQHGAQTAVIMFGGVNYYTGQFFDLRAITAAGHAAGAYVGFDLAHAAGNVPLQLHDWGADFACWCSYKYLNSGPGGPSGIFVHEKHAYHKSLKKKIAQLVGAKESEVCPMNTLTVNLHLLMVSFYKPVPGKFKIIMESGAFPSDQYALESQVRFHGYDPKEAIIEVAPRHGEYILRTEDILAAIAENGEELALVLFGGINYFTGQLFDLAKITEAGHEVGAVVGFDLAHAAGNVPLSLHDWKVDFACWCSYKYQNSGPGGISAIFIHENNPAIKEQTAKILGALPTEVVIMETLTANLHLLMSSFYRPTKDRWKIIIEGKAFPSDHYAVLSQIANHSLDPSALITIDPPSASSPYLSNEHILSVISRHAATTALVLLPGIQFYSGQFFDIELITRHCQSLGITVGWDLAHAAGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHL"
  },
  {
    "reference": "MSSIVNGFSIQKSTKTAYDTQQPCDCWDCSAFTAVVGCGQTDGMDDWEFGETACCFTCPNKKQCAKPDPAECNIGTDSHQRDPLTRVTWNGRGPNVQCVYDVNRINTLEQIDNFKQRFGVHGDYNAVVANYCQQSSDSCITDPETGTKMTKCSRFKSDQKDGELCRGWFNQQPKAVQDTVVQNYCAVNNTPDCKCVNRAQNEVYRELKIGKVINDGCWFTPCANPQSYLLTTEVENPTCPSNFCDIIYNIIKDRDVTIDDVKNDINCVFKPDPPPQPKPQPPPDPPKPPPDPPKPDPPPPPPPKPTPPPDPPKPKPDPVPPPKPTPPPPKPTPPPPIPPQPVPILPIPPVDLKKNWIMLTFVGLVLALVIYPKSRHAIGTHTWRNAAIAVLVGLNAFGLQSYVNNHV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Target a Pro residues compositional bias in the protein's composition for improved properties.2. The designed protein localizes to the virion membrane. The designed protein sequence is ",
    "response#1": "TVGSSIGSTTRTFAGLFSTGGKQNIQLINSNGSWHVNRTALNCNDSLQTGFIAGLMYVNKFNSSGCPDRMSSCKPLHAFDQGWGPLSYANISGSSPDKPYCWHYPPRPCTIVSAATVCGPVYCFTPSPVVIGTTDRKGLPTYAWGANETDVFLLQSTRPPSGSWFGCTWMNSTGFVKTCGAPPCNITPGGSGNSTLVCPTDCFRKHPEATYAKCGSGPWLTPRCLVDYPYRLWHYPCTLNFTIHKVRMYVAGIEHRFNAACNWTRGERCELDDRDRVEMSPLLFSTTQLAILPCSFTTMPALSTGLIHLHQNIVDVQYLYGVSSAIVSWAVKWEYVVLAFLVLADAR",
    "response#2": "SRNLGKVIDTLTCGFADLMGYIPLVGGPLGGAARVLAHGVRVLEDGVNYATGNLPGCSFSIFLLALLSCLTIPAV",
    "response#3": "TTGGAQARAASGFASFFSVGPSQKIQLVNTNGSWHINRTALNCNDSLNTGLLAALFYAHRFNSSGCPERMASCRPIDRFDQGWGPITYVKPASVDQKPYCWHYAPQPCGIVPASQVCGPVYCFTPSPVVVGTTDRSGVPTYRWGENETDVLLLNNSRPLRGNWFGCTWMNGTGFTKTCGGPPCNIGGGGNNTLTCPTDCFRKHPEATYTKCGSGPWLTPRCMVDYPYRLWHYPCTVNFTIFKVRMYVGGVEHRLTAACNWTRGGRCDLEDRDRSELSPLLLSTTEWQVLPCSFTTLPALSTGLIHLHQNIVDVQYLYGIGSAVVSYVIKWEYVLLLFLYLADD"
  },
  {
    "reference": "MSNATQRPLSQLGLTARGGSDPLIHGLATDSRKVSEGFLFAALPGSQVHGARFVPMALELGAAAILTDVDGAALIADQIGDDVALVVVEDPRQSLSGAAALWYGAHPDTMVAVTGTNGKTSVATFVRQIWEALGHAAINVGTTGIEGAWSHPLHHTTPDPITLHAALAEAAEAGITHAAMEASSHGLDQRRLHGVHLAAAGFTNFTQDHLDYHETFDAYFAAKASLFRSVLPEDGVAVINMNDERGVEMRAVAAAKGQEVLTVGLGLGDLALVGTRYDASGQDIRFTWHDTPHVVRLNLIGGFQAENVLLAAGLVIASGEAPNDVFMVLPQLATVRGRMQLAATRDNGATVFVDYAHTPDAVATAIQALRPHVIGRLIAIVGAGGDRDTSKRPLMGAAAHKHADMVIVTDDNPRSEDPDAIRAAVMGGAPGAHNIGDRAEAILRGVDMLQAGDALIICGKGHESGQVVGDQVLPFDDVEQASMSVAALDGRLA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.2. The designed protein should contain at least one Meso-diaminopimelate recognition motif motif to achieve its intended function.3. The protein should be able to bind UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate ligand in a variety of conditions4. The protein contains novel Mur ligase central, Mur ligase N-terminal catalytic domains that confer a unique function or activity.5. The designed protein should be involved in peptidoglycan biosynthetic process, cell wall organization by localizing to cytoplasm and exhibiting UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity.6. The protein should be designed to catalyze the following reaction ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "MTLNELIQAVGLTEATGNTDREITGVTTDSRAVRRGQLFAALPGTRVHGAAFIPMALEKGAGAILTDADGAALAADALAASDAALIVAEDPRAALAQTAAAVYGNPSTRLQLLAVTGTNGKTTVTYLVEAILRAAGHRPAVFGTINYRFADQILSSLHTTPEAIDMLRGVAGFRRQGADALVMEVSSHALEQHRVSGVHFKVGVFTNLTPEHLDYHGNMDDYFASKTRLFTELLQKEGAVVNVEDPYGARLAGLLLSPLTCGSSADCAIYPLDVKTTLEGTSGTVDTPCGTVSFSSPLLGAFNLQNLLCAIGLVIGSGADVDSAISGLQSVAGVAGRMQLAATRKNGAAVYVDYAHTPDAIETALKALRPHVMGRIVIVFGAGGDRDTTKRPLMGAAARAHADVLYVTDDNPRSEEPATIRAAVLAACPDANEVGDRAEAILLGVDALGPGDALLIAGKGHEQGQIVGETVVPFDDRIVAAEAIRG",
    "response#2": "MSLEVTGLHYDSRTVEPGGAFFALRGQALDGHRFIDQALERGAKTIFLEKERELPPGVATVVVDDCRQVMAQVAAALTAWFFGEPARRLRMIGITGTNGKTTSAYLLKSVLESLGQRPGLIGTIENRIGDQWLPVTGTNGKTTVTYLVEAILRAAGHRPAVFGTINYRFADQILSSLHTTPEAIDMLRGVAGFRRQGADALVMEVSSHALEQHRVSGVHFKVGVFTNLTPEHLDYHGNMDDYFASKTRLFTELLQKEGAVVNVEDPYGARLAGLLLSPLTCGSSADCAIYPLDVKTTLEGTSGTVDTPCGTVSFSSPLLGAFNLQNLLCAIGAGLASGVPLPELVAALPQLTAPAGRLERVDNGRGVMALVDYAHTGDALDKVLVTLAALAPRRMITVFGCGGDRDRSKRPVMGEIASRRSDLAVVTSDNPRTEDPDAIIAEVCAGVARVHRRPLTRAEAASGAKGYIVIPDRREAIAFAADVSRGGDLLLVAGKGHEDYQIVGDERLHFDDREHARAALAERQMR",
    "response#3": "MTLRDLISGVAAVGPAPALEVTGLHYDSRTVEPGGAFFALRGQALDGHRFIDQALERGAKTIFLEKERELPPGVATVVVDDCRQVMAQVAAAALFDAPGAKLAVVGITGTNGKTTTTYLVLALTEANDERAAMLTTVEYRIGERSEPAIRTTPEASDTQRFLREALNENCLTAVMETSSQAIDLHRCDWLRFRVAVFTNLTRDHLDYHLTMENYFDAKKKLFDGRIGEKPAASVINVDDEWGAKLANELKANNQRVVTFAQNKTADLTAENIEVSLLSGTSFDLKTPQGSRKITSPLVGKPHVYNMLAAMATALIAGVDLDTVVAGIGSVDVPGRLERVDNGRGVMALVDYAHTGDALDKVLVTLAALAPRRMITVFGCGGDRDRSKRPVMGEIASRRSDLAVVTSDNPRTEDPDAIIAEVCAGVARVHRRPLTRAEAASGAKGYIVIPDRREAIAFAADVSRGGDLLLVAGKGHETGQEIAGVVHPFDDREVAREALDLSEAGR"
  },
  {
    "reference": "MSTNPKPQRKTKRNTNRRPQDVKFPGGGQIVGGVYLLPRRGPRLGVRATRKTSERSQPRGRRQPIPKARRPEGRTWAQPGYPWPLYGNEGMGWAGWLLSPRGSRPSWGPTDPRRRSRNLGKVIDTLTCGFADLMGYIPLVGAPLGGAARALAHGVRVLEDGVNYATGNLPGCSFSIFLLALLSCLTIPASAIEVRNVSGVYHVTNDCSNASIVYEAADMIMHTPGCVPCVRENNSSRCWVALTPTLAARNASIPTTTIRRHVDLLVGTAAFCSAMYVGDLCGSVFLVSQLFTFSPRRHETVQDCNCSIYPGHVSGHRMAWDMMMNWSPTTALVVSQLLRIPQAILDVVAGAHWGVLAGLAYYSMVGNWAKVLIVMLLFAGVDGGTYTTGGAQAHTARRFVSLFSPGPTQHIQLINTNGSWHINRTALNCNDSLNTGFLAALFYTRKFNASGCPERMASCRPIDKFAQGWGPITYAKPPSLDQKPYCWHYAPQPCGIVPASQACGPVYCFTPSPVVVGTTDRFGVPTYSWGENETDVLLLNNTRPPQGNWFGCTWMNGTGFTKTCGGPPCNIGGVGNDTLICPTDCFRKHPEATCTKCGSGPWLTPRCMVDYPYRLWHYPCTVNFSISKVRMYVGGVEHRIDAACNWTRGERCDLEDRDRSELSPLLLSTTEWQVLPCSFTTLPALSTGLIHLHRNIVDVQYLYGIGSAVVSYAIKWEYVLLLFLFLADARVCACLWMMLLIAQAEAALENLVVLNAASVAGTHGILPF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be designed to localize specifically to lipid droplet in order to perform its role in proteolysis more effectively. The designed protein sequence is ",
    "response#1": "EVRNVSGVYHVTNDCSNASIVYEAADMIMHTPGCVPCVRENNSSRCWVALTPTLAARNSSIPTTTIRRHVDLLVGTAAFCSAMYVGDLCGSVFLVSQLSTFPPRRHETVQDCNCSIYPGHVSGHRVAWDMMMNWSPTTALVVSQLLRIPQAVVDIVAGAHWGVLAGLAYYSMVGNWAKVLIVMLLFAGVD",
    "response#2": "GIPLTNLAIPGSHDSFSFYIDEASPVGPEQPETVQNFVSVFGTVAKKLMRKWLATQTMNFTSQLEAGIRFFDLRISTKPRDPDNELYFAHGLFSATVREGLEQISTFLASHAREVVFLDFNHFYGVQNLHHEKLVQMLRTVFGDRLCPVVFAQEVSLKYLWEKEYQVLVFYR",
    "response#3": "ELDLTCNITTDRADDVRPEVTWSFSRMPDSTLPGSRVLARLDRDSLVHSSPHVALSHVDARSYHLLVRDVSKENSGYYYCHI"
  },
  {
    "reference": "MXVKGIRRNYQHWWKWGTMLLGMWMICSAVEPLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPIDPNPQEVVLENVTENFNMWENNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCSNYVKNDTNTTNSNNNDTTKGVREEGELKNCSFNVTTSIRNKMKQEYALFYKLDVEQIDNTSYTLINCNSSVITQACPKVSFEPIPIHYCTPAGFAILQCNDKKFNGTGPCSNVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVIRSENFTNNAKTIIVQLNASVEINCTRPNNNTRRGIHIGLGRTFYATGEIVGNIRQAHCNINKTKWDNTLRQLVIKLKEQVGNKTIAFDQPSGGDPEIVMHSFNCGGEFFYCNTSQLFNRAWNGSERSNNTEGNTTITLPCRIKQIINRWQEVGKAMYAPPISGQIRCSSNITGLLLTRDGGNGNETENGTEIFRPGGGNMRDNWRSELYKYKVVKIEPLGIAPTRAKRRVVQREKRAVGTIGAMFLGFLGAAGSTMGAASVTLTVQARLLLSGIVQQQNNLLKAIEAQQRLLQLTVWGIKQLQARLLAVERYLKDQQLLGIWGCSGKLICTTAVPWNTSWSNKSQDEIWHNMTWMQWDREINNYTSLIYTLLEESQNQQEKNEQELLELDKWASLWNWFDITQWLWYIKIFIMIVGGLIGLRIICSVLSIVNRVRKGYSPLSFQTHLPSPRGPDRPGGIEGEGGEEDRGGSSPLVDGFLTLIWVDLRSLCLFSY",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Incorporate a signal peptide in the protein design.2. The designed protein have Human immunodeficiency virus 1 envelope glycoprotein Gp120, Retroviral envelope protein GP41-like domains with specific sequence motifs that are crucial for its activity.3. The protein should be designed to localize specifically to host cell endosome membrane, viral envelope, host cell plasma membrane in order to perform its role in fusion of virus membrane with host endosome membrane, positive regulation of establishment of T cell polarity, mitigation of host antiviral defense response, virion attachment to host cell more effectively.4. The protein's functional design necessitates the inclusion of a Di-leucine internalization motif motif.5. The protein must exhibit the following characteristics: Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes. The designed protein sequence is ",
    "response#1": "MGAASLTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKIICTTAVPWNSTWSNRTFDEIWKNDNMTWIKWEREISNYTNQIYELLTESQNQQDRNEKDLLELDKWASLWNWFGITNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTPISWTS",
    "response#2": "MRVKGIRKNYQHWWRWGTMLLGMLMICSATEKLWVTVYYGVPVWKDANATLFCASDAKSYKTEAHNIWATHACVPTDPNPQELELKNVTENFNMWKNNMVEQMHEDVISLWEQSLKPCVKLTYLCVTLNCTNATSKDTSPTATSVNSSSIGEVKNCTFNVTTELIDKTKKEHALFYRLDIVEIGEKNNNTNWTTYRLINCNTSKITQACPKXTFQPIPIHYCAPAGFALLKCNDKRFNGTGPCTNVSTVQCTHGIMPVVSTQLLLNGSLAEEEIMIRSENLTDNVKNIIVQFNKSISITCVRPGNNTRRSIHFGPGRAFYANDITGDIRKAHCNISGGNXSNTLHQVAKKLRNLYNTTKIIFQPSSGGDPEIXTHSFNCDGEFFYCDTSKLFNGTWNNGTWENSTWNNTDITIPCRIKXIINMWQGVGKAIYAPPIAGQINCTSNXTGLILTRDGGXSNDSHSNKNETFRPGGGNMKDNWRSELYKYKVVRIEPLGVAPTKAKRRVVEREKRAALGALFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLQDQQLLGIWGCSGKLICTTNVPWNSSWSSKSQDEIWGNMTWMEWDREINNYTGIIYKLLGESQIQQENNEKELLALDKWNNLWNWFGITEWLWYIKIFIMVVGGLVGLRIIVAVFSMVNRVRQGYSPLSFQT",
    "response#3": "MRVKGIRKNYQHLWRWGTMLLGMLMICSAAEQLWVTVYYGVPVWKDANATLFCASDAKSYKTEAHNIWATHACVPTDPNPQELELKNVTENFNMWKNNMVEQMHEDVISLWEQSLKPCVKLTYLCVTLNCTNATSKDTSPTATSVNSSSIGEVKNCTFNVTTELIDKTKKEHALFYRLDIVEIGEKNNNTNWTTYRLINCNTSKITQACPKXTFQPIPIHYCAPAGFALLKCNDKRFNGTGPCTNVSTVQCTHGIMPVVSTQLLLNGSLAEEEIMIRSENLTDNVKNIIVQFNKSISITCVRPGNNTRRSIHFGPGRAFYANDITGDIRKAHCNISGGNXSNTLHQVAKKLRNLYNTTKIIFQPSSGGDPEIXTHSFNCDGEFFYCDTSKLFNGTWNNGTWENSTWNNTDITIPCRIKXIINMWQGVGKAIYAPPIAGQINCTSNXTGLILTRDGGXSNDSHSNKNETFRPGGGNMKDNWRSELYKYKVVRIEPLGVAPTKAKRRVVEREKRAIGLGALFLGFL"
  },
  {
    "reference": "MAKRVVLGMSGGVDSSVCAYLLQQQGYEVVGLFMRNWDSFLNNDFLGNEAISQDVCPQEQDYADAKEVAKQLNIPLYRVDFIKEYWDHVFSYLIEEYKIGRTPNPDIFCNKYIKFNFFAQVAFSKYQADFIAMGHYAYCENGELFKAKDDSKDQSYFLAQLSNEQLKKVIFPLANLTKQEVREIAKNLNLATATKKDSTGICFIGERKFTKFLENYIPAQPGKIIDIATKQVVGSHIGTMYYTIGQRKGLNLGGFSKPYFVVGHNLEKKVLYVANEDFPEYLLSDKLIAIGFNQIAYKFDTNNLSAKFRYRQKDIPIKLKLLANSEIEVSYNDTEAVTPGQEIVIYDGNKVVGGAIINEVFYKNQKKTYI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The Nucleophile, Cysteine persulfide intermediate of the protein must be engineered to enable effective catalysis of AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in   tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-   cysteinyl-[protein].2. A protein that contributes to tRNA modification by functioning in cytoplasm with sulfurtransferase activity, tRNA binding, ATP binding.3. The protein must exhibit the following characteristics: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34..4. The designed protein should have a high specificity for ATP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "TLSMADLSWTLPDAPAPGEYSAKNRYRMADAACTLSPIVDGQASLTFQEKQWAVTPGQSAVLYHGDICLGGGIEA",
    "response#2": "MKWLKDWNIVDESRLLGCDDVIVKIRYRKQENHCSVTITPEGLLHIRLHEPLSAIAEGQAAAFYKDGLLLGGGIS",
    "response#3": "TLSMADLSWTLPDAPAPGEYSAKNRYRMADAACTLSPIVDGQASLTFQEKQWAVTPGQSAVLYHGDICLGGGIEVIAQAE"
  },
  {
    "reference": "MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFINDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIFANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFPHVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAEGKGKTKKESEQRAAESAYKQLKQIK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.2. For general function, the protein need meet that Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S); Digests double-stranded RNA3. The protein should be optimized for cytoplasm-specific folding and stability, while maintaining ribonuclease III activity.4. The designed protein must have a active site active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "YQDLLFGARTLSNSATFRDLYCLHVLNHILKTRDRVLKNNSRLQKEPESDLELRDQGFTRPKVLIILPTRQACVRVMESITKLYQAEQQENKARFYETFSAADDKSWEHKPDDFRELFGGNDDDMFRLGLKFTRKTIKYFSQFYNSDIILASPLGLRMVMDKEDGEKQDFDFLSSIEIAIVDQADALLMQNWEHTEYVFSHLNLQPKESHGCDFSRVRNWYLDDQAKYVRQTLTFSSFITPEINALFSSRMQNTAGKV",
    "response#2": "IKEVEAKLKFTFTQPQLLVTALTHPSYRNETVTITEDSERLEFLGDAVLCLIVTEHLFLLFPSMDEGTLSTARAALINAVSCCQYTDALGLGEYLLIGKGERIQNERGRTSAYANLFEAILGAVYLDGIRIRRTS",
    "response#3": "LPRLGRILGYQFQQIALLEQALTHRSAASKHNERLEFLGDSILSIVISDALYHQFPKATEGDLSRMRATLVCGKMLAEIAKEFKLGDYLKLGPGELKSGGFRRESILADAMEAIIGAIYLDAGRLTGAQAVEKATA"
  },
  {
    "reference": "MSANHQQPDSAKKVNFGHQEVSPEEKTERVNAVFRTVAKRYDVMNDIISFGSHRIFKRMVLHMSGVRAGGKVLDLAGGTGDMAALFADAVGSAGRVVLTDLNEAMMRVGRDRLLDHGLTQIDFCRAPAEALPFADQQFDCACISFGLRNFTDKDLALRELLRVLKPGAALIVLEFSKPTDPLLESAYKAFQTFWPPIGRALVGDAQPYQYLVDSIRVHPDQKALRQMFSDAGFVDAEYHNLLGGIAAIHRGRKPTEVPTTD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should be able to enhance the efficiency of methylation. The designed protein sequence is ",
    "response#1": "MPPRHLADLAMPARAEAVAELGEKPFRAKQLSHHYFSRLTTDAAEMTDIPAGSRAELVEALMPRLLTEIRALDCDAGATRKTLWRAHDGTLLESVLMRYPGRVTLCISSQAGCGMACPFCATGQGGLDRNMSTAEIVDQVRSAAAVMRDGLMPGPEGEPREGRLSNVVFMGMGEPLANYNRVVEAVRRITEPAPRGLGIAQRSVTVSTVGLAPAMRKLADEGMQVRLALSLHAPDDELRNELVPVNNRWPVDEILSAARYYLDRTGRRISVEYALIRDMNDQPWRADLLASTLREHLGHLAHVNVIPLNPTPGSKWDASPKPVEQEFVRRVNAGGVTCTVRDTRGQEIAAACGQLAGSL",
    "response#2": "MPPRHLADLAMPARAEAVAELGEKPFRAKQLSHHYFSRLTTDAAEMTDIPAGSRAELVEALMPRLLTEIRALDCDAGATRKTLWRAHDGTLLESVLMRYPGRVTLCISSQAGCGMACPFCATGQGGLDRNMSTAEIVDQVRSAAAVMRDGLMPGPEGEPREGRLSNVVFMGMGEPLANYNRVVEAVRRITEPAPRGLGIAQRSVTVSTVGLAPAMRKLADEGMQVRLALSLHAPDDELRNELVPVNNRWPVDEILSAARYYLDRTGRRISVEYALIRDMNDQPWRADLLASTLREHLGHLAHVNVIPLNPTPGSKWDASPKPVEQEFVRRVNAGGVTCTVRDTRGQEIAAACGQLAGSTRL",
    "response#3": "SSQVGCQLGCTFCATGLSGFKRDLRTSEIVAQVLYMEQKIGKEVNNIVFMGMGEPFLNNEAVFKSIEILHEKKGRNLGIRHFTISTAGIPEGIKKLANSGLDVKLSVSLHAADDEKRSELMPINRRFPLKELFNILDYYQKETGNRITFEYILIDGINDSLEDARKY"
  },
  {
    "reference": "MSLHIVILAAGQGKRMNSALPKVLQPLGDRPLLRHVVETAQALDPAAIHVVYGHGGERVQQALTDMPVNWVYQAEQLGTGHAVGQAMDHVPDDARVLVLYGDVPLVRANTLSNLVAAADNGALALLTVVLADPTGYGRIVRDDNNRVQRIVEERDADAGERHIQETNTGLLCAGAAALRDWLSRLTDDNDQGEYYLTDCVALAAADGAPVTAGQAATEAETQGINNKLDLARAERLFQRRQADALMAAGLTLRDPARFDLRGALRAGRDSEIDVGVVVEGQVTLGDNVVIGPHVVLKNCAIDDDTRIEAHSVIEGATIGRRCAIGPFARLRPDTHVADRAKIGNFVETKKTRLGEASKINHLSYVGDAEVGAGVNIGAGVITCNYDGANKHITRIGDDAFIGSDCQLVAPVSVGAGATIGAGSTVTKDAPAGALTLSRVRQSTVTGWRRPTKQA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have UDP-N-acetylglucosamine diphosphorylase activity within cytoplasm to regulate UDP-N-acetylglucosamine biosynthetic process, lipid A biosynthetic process.2. The Mg(2+), UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA binding site should be located in a region of the protein that is accessible to the ligand.3. The Proton acceptor of the protein must be engineered to enable effective catalysis of acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.4. The presence of MobA-like NTP transferase domains is critical for the interaction of the designed protein with its intended targets.5. We require a protein that can specifically bind to the metabolites produced in the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway. The designed protein sequence is ",
    "response#1": "MTPILGAGLGTRFKSKRAKLLHEAGGRKLIEHAVRTAAKLSASKIFAIVGYQADAIQKAITEADSKLAGGKVRFLLQDKPLGTGHALKCGQKELRAAAPLLLVYCGDTPLLRPETLRDFLSYHRRLKATATVMTAIMADPSGYGRMVRSADGQVDAIVEHKSASADILAIREINTGIYCFDTKELFSQLDRLKPDLDEGEGRLTEIRSINDRLEMSEINAAWYVFDSSVLLDAIKAIEAGGKRGEYYITEAIKILSDKGIRVEAHRVEDPMELMGVNDRVQLSEAEKAMKKRINESHMRAGVTVIDPATTWIDDDATLAPDVTILPNTHILRATKIAEGAIIGPDTTLVDCEVGEDAIVRRSDATLAVIGAEATVGPFSFLRPNTVLGARGKIGAYVETKNAEIGEGSKVPHLSYVGDATIGRGVNLGASTITANYDDVHKHRTVVEDEVHTGSHTTLVAPVRLGAGAKTGAGAVVRKDVPAGSLSMSIAPGVNIIDPDSVYISPMSVIGSDTVIYPGVFIRGEVKIGKNCSIGPYCVIEDCVISDGCDIKPFSCLYSSRAENSVSIGPFSHLRPQTYLCEKAKVGNFSEVKKSKIGRGSKVPHLSYVGDTVMGEKVNVGAGTITCNYDGINKNKTVIEDLAFIGSNTNLVAPVKVGKGALIAAGSTITEDVPPETLAIARSRGMPARIIKK",
    "response#2": "VIVLAAGAGTRMRSKTPKVLHTLAGRSMLSHSLHAAAAVEPTELVTVIGHDREAVGAAVDEVAKELGRAVVTAVQHEQNGTGHAVSCGLTALPVDFDGTVLVTAADVPLLDADTLRALIDAHTTAPAAAVTVLTSTAPDPTGYGRLVLGADGTPVGIVEQKDATPEQREITEINSGVYVFDAGVLRTALGQLGTDNAQGEKYLTDVLGIARADGGRVGAHLAADRWLTEGVNDRVQLATLGAEYNRRVLGRWMRAGVTVIDPATTWIDDDATLAPDVTILPNTHILRATKIAEGAIIGPDTTLVDCEVGEDAIVRRSDATLAVIGAEATVGPFSFLRPNTVLGARGKIGAYVETKNAEIGEGSKVPHLSYVGDATIGRGVNLGASTITANYDDVHKHRTVVEDEVHTGSHTTLVAPVRLGAGAKTGAGAVVRKDVPAGSLSMSIAPGADVEIAADGVALRDPSRLDVRGSLTCGHDVEIDVGCVFEGDVELGEGVRVGPHCVIRDSHIGAETVIEPHSVIEGAVVAGHNRIGPFARLRPGTRLAVGAKVGNFVETKNAEVGEGSKINHLSYVGDARLGRDVNVGAGTITCNYDGANKHRTEIGDEAFIGSNTALVAPVSVGRGATVGAGSTIDRDVADNALAVERSPPRKRD",
    "response#3": "VIILAAGEGTRMKSNTAKVLHRVAGRPLLGHVLAAVEHLQPKEIRIVVGSGRQSVEEYISEVLPSATTIFQEKRGGTGHATQLALAGNDIRGTVLVLAGDTPMLTGQSLAAFLQVHNSGNFTASVLTAEHPDPTGYGRIVRTASDEALGRIIREAGRFVEIVEYKDATEAERAIRLCNSGMMAFDAAFLMECLDKVTNDNAKGEYYLTDVVGLAVADGRRVAAVEAPEAETLGVNTRAELAEAEAAMRARIRERHMLAGVTIYQPNSCVIDADVQIGADTVLEPFVQLLGATTIGPDCHIESYSIIRDSNLAAAVHVKPGCVIEDSEVKRAAVLGPYAHLRTGSRIGEQSHIGNFVETKNTTLGRGSKANHLTYLGDAEIGDEVNVGAGTITCNYDGFAKHTTVLEDGAFIGSDSTLVAPVRIGKGAYVGAASCITEDVPDRALALARSRQATKEGWAARFRAARRAKKSKDK"
  },
  {
    "reference": "MSQKSWTESNFTKRECVFIIPVSKDPHRCLPGCQICQQLVRCCCGRLVRQHVGFTASLAMKYSDVKLGEDINSELETWSVEKHTEESPTDAYGIINFQGGSHSYRAKYLRLSYDAKPEDILCLMLKEWQMDLPKLVISVHGGMQNFELLPRIKQVVGKGLVKAAITTGAWILTGGVNTGVAKHVGDALKEHASSLPRKICTIGIAPWGVIENRNDLIGKDVVVPYQNLLNPLSKLNVLNNHHSHFLLVDDGTVGKYGAEVNLRRKLEKHVNLQRIHARIGQGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADILAYIHKQTEEGGRLTDGVEDEIISTIKKTFNFSQNEAIHLFQTLMECMKNKELITVFHIGSEEHQDIDVAILTALLKGSNACAFDQLVLTLAWDRVDIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVEFVKLLIENGVSMHKFLTIPRLEELYNTKQGPINPALFHLVRDVKQGNLTADYKITLIDMGLVIEFLMGGTYRCTYTKKRFRIIYNNLHGNNRRPGRHCSTTNVPQLRKNHDSFSNRADKKEKMRHNHFIKTAQPYKPKLDATVEQGKKKRCKDEVVDIDDPEMRRFPYPFNELLVWSVLMKRQKMALFFWQHGEESMAKALVACKLYRSMAYEAKQSDVVDDASEELKQCSSEFGQLAVDLLEQAFRQDETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must have at least one Alpha-type protein kinase domain.2. The designed protein should have a binding site that is specific to the target ATP, Zn(2+) ligand.3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.4. The designed protein should have at least Helical transmembrane domains to ensure proper localization.5. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.6. The ideal protein would be one that plays a critical role in exocrine pancreas development, skeletal system development, renal system process, protein tetramerization through calcium channel activity within plasma membrane.7. The Proton acceptor of the protein must be engineered to enable effective catalysis of ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-   threonyl-[protein]. The designed protein sequence is ",
    "response#1": "LDCGLPSNEPPYIEPVTGLVFSSDADHIPSGISGRIQKNLEAVHIKPYLFLRYFPDGLRNCYTLDVLQNRRYMIKAVFVYGNYDGYNDYPSFDLYLGPNKWVRVDLEGKVNGSVEEIIHIPSSNSLQICLVKTGNSLPFISALELRLLRNDTYVVQDVSLKHLFRRYYRQSDRLIRYPDDVYDRVWSPFFLPEWTQITTSLDVNNSNNYEPPKAALTSAATPGDNGTRLTIIWTLDNPDEQIHLYVHFAELEPVGENTDEALRTLFTRTFYFVVNGKISYDESITPLDLAVSTVETVVNKCDGGNCSLQLVRSEASPGVRVPLVNAMEAFLFDNSKMSYETQITPRPQPESVSCVLQEPKRNLSFFQMRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNAMASTAQQLKAKLDFFKTSIQIDLEKYREQTEFGITSDKLLLAWREMEQAVEQCGRENDVKVLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQRTEGDSQDMVRLLLQAIQSFEKKVRVIYSQLSKTVVCKQKALELLPKVEEVVRLMNEDEKTVVRLQEKRQKELWNLLKIACAKYARLSHDSRPESILRLMLKEWHMELPKILISVHGGVQNFELHPRIKQVVGKGLIKAAVTTGAWILTGGVNTGVAKHVGDALKEHCSRSSKKICTIGIAPWGVIENRNDLIGRDIIAPYQTLLNPLSKLNVLNNLHSHFLLVDDGTVGKYGAEVKLRRDLEKHINLQRIHARIGQGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADILAYVHKQTEEGGGLPDGVETDIIATIKKTFNFSQSDAIHLFQTLME",
    "response#2": "VMGDVLGEGSYGKVKEAMNSENLCRLAVKILTKRKLRRIPNGEQNVTREIHLLKQLKHTNVVGLVDVLYNEEKQKMYLVMEYCVGGLQEMIDDQPDKKMPLFQAHGYFQQLIDGLEYLHGCRVIHKDIKPGNLLLTLDQTLKISDFGVAEQLDLFSPDDTCTTGQGTPAFQPPEIANGHETFAGFKVDIWSSGVTLFNLATGLYPFEGDNIYRLLENIGRGQWVAPDWLYEMDAELANLIYGMLQADPSARLSLQQIRQNTWAKYVRLSHDSRPEAILRLMLKEWQMELPKIVISVHGGIQNFELHPRIKQVVGKGLIKAAVTTGAWILTGGVNTGVAKHVGDALKEHSSRSARKICTIGIAPWGVIENRNDLIGRDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVNLRRELEKHINLQRIHARIGQGVPVVALIFEGGPNVILTVLEYLQETPPVPVVVCEGTGRAADILSYVHKQTEDDGGLPDGVETDIIATIKKTFNLSQNDAIHLFQTLMESLLNLFDNSKMSYETQITPRPQPESVSCVLQEPKRNLSFFQMRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNAMASTAQQLKAKLDFFKTSIQIDLEKYREQTEFGITSDKLLLAWREMEQAVEQCGRENDVKVLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQRTEGDSQDMVRLLLQAIQSFEKKVRVIYSQLSKTVVCKQKALELLPKVEEVVRLMNEDEKTVVRLQEKRQKELWNLLKIAC",
    "response#3": "EMKERLGTGGFGNVIRWHNQVTGEQIAIKQCRQELSPKNRDRWCLEIQIMRRLNHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRRYLNQFENCCGLREGAILTLLSDIASALRYLHENRIIHRDLKPENIVLQQGEKRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGTVKFSSSSPFPNNLNSVLAERLEKWLQLMLTWQPRQAKYVRLSYDTKPEAILQLMLKEWQMELPKLVISVHGGMQKFELHPRIKQLLGKGLIKAAVTTGAWIITGGVNTGVAKHVGDALREHASRSSRKICTIGIAPWGVIENRNDLVGRDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVKLRRELEKTINLQRIHARIGQGVPVVALVFEGGPNVILTVLDFLQESPPVPVVVCEGTGRAADILAYVHKQTEEGGNVPEGAEPEIISTIKKTFNFGQSEAVHLFQTLLEE"
  },
  {
    "reference": "MTAEAPPLGELEAIRPYPARTGPKGSLVYKLITTTDHKMIGIMYCVACISFFFIGGLLALLMRTELAAPGLQFLSNEQFNQLFTMHGTIMLLFYATPIVFGFANLVLPLQIGAPDVAFPRLNAFSFWLFVFGATIGAAGFITPGGAADFGWTAYTPLTDAIHSPGAGGDLWIMGLIVAGLGTILGAVNMITTVVCMRAPGMTMFRMPIFTWNIMVTSILILIAFPLLTAALFGLAADRHLGAHIYDAANGGVLLWQHLFWFFGHPEVYIIALPFFGIVSEIFPVFSRKPIFGYTTLVYATLSIAALSVAVWAHHMFATGAVLLPFFSFMTYLIAVPTGIKFFNWIGTMWKGQLTFETPMLFSVGFMVTFLLGGLTGVLLASPPLDFHVTDSYFVVAHFHYVLFGTIVFATFAGIYFWFPKMTGRLLDERLGKLHFWLTFIGFHTTFLVQHWLGDEGMPRRYADYLPTDGFQGLNVVSTIGAFILGASMFPFVWNVFKSWRYGEVVTVDDPWGYGNSLEWATSCPPPRHNFTELPRIRSERPAFELHYPHMVERLRAEAHVGRHHDEPAMVTSS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a low Km value for 4 Fe(II)-[cytochrome c] + 8 H(+)(in) + O2 = 4 Fe(III)-   [cytochrome c] + 4 H(+)(out) + 2 H2O.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. The designed protein should have a binding site that is specific to the target Fe(II)-heme a, heme a3, Cu cation ligand.4. The designed protein should be involved in respiratory electron transport chain, electron transport coupled proton transport by localizing to plasma membrane, respirasome and exhibiting heme binding, metal ion binding.5. A protein that is capable of catalyzing reactions within the oxidative phosphorylation, Energy metabolism pathway with high efficiency and specificity.6. The protein must exhibit the following characteristics: Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water; CO I is the catalytic subunit of the enzyme. The designed protein sequence is ",
    "response#1": "MFHNFHILSLSSYAYNLFFASAGMLSSLVMFFKFGLYELFIFTLFSVLFISFAWGKDIAMEGLSGYHNFFVMDGFKFGVILFVFSEFMFFFCIFCTFFDAALVPVHELGETWSPFGMHLVNPFGVPLLNTIILLSSGVTVTWAHHSLLSNKSCTNSMILTCLLAAYFTGIQLMEYMEASFSIADGVFGSIFYLSTGFHGIHVLCGGLFLAFNFLRLLKNHFNYNHHLGLEFAILYWHFVDVVWLFLFVFVYWWSHPKAWKWYDYFTFNVDHKVIGIQYLVTAFIFYLIGGLMAVAMRTELATADSDFLDPNLYNAFLTNHGTIMIFLWVVPAAIGGFGNYLVPLMIGARDMAFPRLNALAFWLNPPAGALLLASFLFGGAQAGWTSYPPLSTITATTAQSMWILAIILVGTSSILGSVNFIVTIWKMKVPSMRWNQLPLFCWAMLATSLLALVSTPVLAAGLILLLFDINFGTSFYKPDAGGNVVIYQHLFWFYSHPAVYLMILPIFGIMSEVIPVHARKPIFGYQAIAYSSLAICCVGLFVWVHHMFTSGTPPWMRMFFTISTLIVAVPTGVKIFSWVATLWGGKIRLNSAMLFAIGLMSMFVLGGLSGVTLGTAPVDIHVHDTYYVVAHFHYVLFGGSVFGLYAGIYHWFPKMTGRLLDERLGILHFVLTLIGTNWTFLPMHELGLKGMPRRVAMYDPQFEPVNLICTIGAFVLAFSIIPFLINIIWSWNKGKIAGDNPWGGLTLEWTTSSPPLIENWEVLPVV",
    "response#2": "MWKERWFLSTNAKDIGTLYLMFRYFSGLVGTAFSVLIRLELSAPGVQYIADNQLYNSIITAHAILMIFFMVMPALIGGFGNFLLPLLVGGPDMAFPRLNNISFWLLIPSLLLFVFASIIENGAGTGWTLYPPLASIQSHSGPSVDLAIFGLHLSGISSLLGAMNFITTIINMRSPGIRLHKLALFGWAVLITAVLLLLSLPVLAGAITMLLTDRNFNTSFFELAGGGDPIFIQHLFWFFGHPEVYILIVPGFGIISTVISANSSKNVFGYLGMVYAMMSIGILGFVFWSHHMYTVGLDVDTRAYFIAATLIIAVPTGIKIFSWLATCYGGSLNLTPAMLFALGFVVMFTIGGLSGVVLANASLDIAFHDTYYVVAHFHYVLSMGAVFALFSGWYFWIPKLLGLSYDLFAGKVHFWILFVGVNLTLFPQHFLGLQGMPRRIGDYPDAFAGWNLISSFGSIVSVVATWYFLNILYLQLTQGSPVSRYPWLTPQYFSDLFQHLFTRNNSSLEWCLNSPPKPHAFDCLPVQL",
    "response#3": "MWDYVKLVALGVVAAIAAYAASQARDLPYMVNMVEVALAAVIALIWVLRTMGDPKPSKDEYFDGVIRAGVIATTFWGIVGFLVAVVIAFQLAFPALNLEFGNGMLNFGRLRPLHTSAVIFAFGGNALIASAFYVVQRTSAARLFGGTALGWFVFWGWQLIIVTAATSYLLGGSQGKEYAELNWHLDILVAVVWVAYLIAFLGTIFKRKEPHIYVANWFYLSFIVTIAMLHIVNNLAVPVSIFGTKSVQLMAGVQDAMTQWWYGHNAVGFFLTAGFLGMMYYFVPKQAERPVYSYKLSIVHFWALIFLYIWAGPHHLHYTALPDWASTLGMVMSVILWMPSWGGMINGLMTLSGAWDKLRTDPVIRMMVVSIGFYGMSTFEGPMMSIKAVNSLSHYTDWTIGHVHSGALGWNGMITFGMLYFLTPRLWGRSGLYSLKLVSWHFWLATIGIVLYASAMWVTGIMEGLMWREVDAQGFLVNAFADTVAAKFPMYVVRGVGGVLYLLGGLIMAYNLWATVAKQPKTANLAVAVPAV"
  },
  {
    "reference": "METRHPPPPPQLYPSSCWFLLALILEPSANSSGPPLISVASSMAGRGRRGCSGERRRRRRPARARKWRRKRSGHRLRRLYIPAPAANQRRPLAPRRSAPGGFRRACSTRPPPGGRPPPPRPRPRPRSQSRPRARPVRVRRWYTRALPRGLECGVQPPSPPAPHLYVLGALPAAGPGTKRPSLPRAGTGRQTGTGRPRRRGGGGGGGGGVGVVTCNPETPFRRRGGRSGGGHSRRGTPHRWGPAAAALTSRDPCRPGPARPGPPRSRRAERPPPPPPPPRSPGEPRAPRAAAAAAAAMGVLPVPAEVRAILLDIEGTTTPIAFVQETLFPYIKDNVKEYLRAHWEEEECQRDVGLLRKQAQEDSSLDGAVPIPLESGSGEEELERVIQAVVDNVHWQMSLDRKTTALKQLQGHMWRAAYATGHVKGELFEDVVPAIRKWREAGMKVYIYSSGSIEAQKLLFGYSTEGDILELFDGHFDTKIGPKVESESYRRIAATIGCATNNILFLTDVPREANAAEEADTHVAVVIRPGNAGLTDDEKSYYSLISSFTELFLPSST",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Ensure the presence of a signal peptide within the protein.2. The designed protein's functionality will benefit from Pro residues compositional bias in its structure.3. The desired protein should be able to perform 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-   dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate4. A protein that can perform acireductone synthase activity, 2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity, magnesium ion binding in cytoplasm, nucleus to support L-methionine salvage from methylthioadenosine.5. The protein should be optimized for activity in L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6, Amino-acid biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.6. The designed protein should have a binding site that is specific to the target Mg(2+) ligand. The designed protein sequence is ",
    "response#1": "MSRELVAIGARMDARGWVPASAGNLSARCTADSIAITRSGVHKGRLEAADIIEVWLDGTPLRAGDRPSAETQLHCQVYERLPAAGAVLHGHSVAATALTLADNSPGIVLEGYEILKAFPGIATHETRIVLPVFDNDQDMRRLAAAIAPAFAAPPIGYVLRGHGIYAWGEDVERCFWRLEALEFLLSESEELIVDGIEGVRQLIQVHDRPLLMSIFKGVIGRDLTFLREQLEAQLAGGIDLVKDDEILYDNPLTPALDRARIGRDVLNAHYEKTGKRALYAITLSGSVFGLKDQTKRLIEAGATAFLLNTFTYGLDVLRELAADPDISVPIFNHPALSGALIASPEYGIAAPVLLGTLPRLAGADLTLFPSPYGNVALAKDLARGIAVEATRIGETKTILPVPSAGIHPGLVAQLVHDFGTDSVINAGGGVHGHPQGAAAGVLAFRQALDAALAGESLTSAATRQEELRVALDAL",
    "response#2": "LSGTDLAKEIKIRLQEEVKHLMETNKGFAPGLAIVQVGDREDSNVYIRMKLKAASEIGIDAKHIRFPSSITEDELLNGINTLNEDINVHGIIVQMPLDSSNKIDNNKATDAVSPLKDVDGNLGCGMVSSTNAPLKDVDGLHTINAGKVARGVLDDSFIPCTPHGCLELIKKSGITIAGSRAVVVGRSRIVGMPMAELLIWNNATVTTCHTKTKEMDKVISEADILVVAAGRPQMIKGDCIKPGAVVIDCGINSIPDSSRKSGQRLVGDVDFDSAKLKASHITPVPGGVGPLTVAMLMHNTVQAPLKDVDGLHTINAGKVARGVLDDSFIPCTPHGCLELIKKSGITIAGSRAVVVGRSRIVGMPMAELLIWNNATVTTCHTKTKEMDKVISEADILVVAAGRPQMIKGDCIKPGAVVIDCGINSIPDSSRKSGQRLVGDVDFDSAKLKASHITPVPGGVGPLTVAMLMHNTVQAEAAAK",
    "response#3": "LSDLCRHFYGLGWVSGTGGSITIKVHDESIPKPQQLIVMSPSGVQKERMVPEDMYVLSPSGCFLSSPSPKPYPNKPPKCSDCAPLFMKAYLMRNAGAVIHSHGMESCIVTMIDPLSKEFRITHMEMIKGIQGHGYYDELVVPIIENTAHERELTDALAEAIEAYPKTTAVLVRNHGIYIWGDSWIHAKTQAECYHYLFDGSSEAIGIEGVRQLIQVHDRPLLMSIFKGVIGRDLTFLREQLEAQLAGGIDLVKDDEILYDNPLTPALDRARIGRDVLNAHYEKTGKRALYAITLSGSVFGLKDQTKRLIEAGATAFLLNTFTYGLDVLRELAADPDISVPIFNHPALSGALIASPEYGIAAPVLLGTLPRLAGADLTLFPSPYGNVALAKDLARGIAVEATRIGETKTILPVPSAGIHPGLVAQLVHDFGTDSVINAGGGVHGHPQGAAAGVLAFRQALDAALAGESLTSAATRQEELRVALDAAHPRA"
  },
  {
    "reference": "MDAKKIFKGPIIWIVLASLLLLLIVPALTQGSNARVDTNVGIQLLKDDKASQAKIEDGEQRVDLTLREDLELDGRDLGKNVYFYFSEARAEDVVQEVNSSSLDGFTDQPVQSNWLLSMLGFMIPFILIALLFWFLLSRMQGGGGKVMQFGKSRAKLITKDMPQVTFADVAGAEEAVEELHEIKEFLAEPAKFQAVGAKIPKGVLLYGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFEQAKTNAPAIIFVDEIDAVGRHRGAGIGGGNDEREQTLNQMLVEMDGFDSTTNVILIAATNRPDVLDPALLRPGRFDRQIPVEAPDLNGRAKILEVHAQGKPMVPGIDLRSLAKRTPGFTGADLANVLNEAALLTARSNAQLIDDRALDEAVDRVMAGPQKRTRLMKEHERKVTAYHEGGHALVAAGLRNSAPVTKITILPRGRALGYTMVVPEDDKYSVTRNELLDQLAYAMGGRVAEEIIFHDPSTGASNDISKATDTAHKMVTEYGMSNRVGAVKLGSGGSEPFVGRDMGSSREYSEQVAALVDEEVRILLDNAHAEAYWILNENRDILDRLAYELLEKETLNQEQIAEIFAEVRRREPRPVWLSAEDRPVSDIPPVLSPSERRAQLADGQSGEGGSETPVDPESTSDSLGPTVDPTPGGQLPGDGDGGAPGGPSTGPTLP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.2. The protein should be able to bind Zn(2+) ligand in a variety of conditions3. The protein must exhibit the following characteristics: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins.4. The designed protein should have ATP binding to facilitate proteolysis, cell division. The designed protein sequence is ",
    "response#1": "VLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSEFVEMFVGVGASRVRDLFENAKKNAPSIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFEGNEGVIVIAATNRADVLDPALLRPGRFDRQILVHMGKI",
    "response#2": "IPKGVLLYGPPGTGKTLLARAVAGEAGVPFFTISGSDFVEMFVGVGASRVRDLFEQAKENSPCIIFVDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFGDRQGIILIAATNRPDILDPALLRPGRFDRQIPVTNPGVIILAATNRRDVLDPALLE",
    "response#3": "VLLLGAPGVGKTLLARAVAGEADVPFLSVSGSDFVEMFVGVGAARVRDLFEQAKKYQPCIVFVDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFDVSTGIIVMAATNRPDILDPALLRPGRFDRHVIE"
  },
  {
    "reference": "MADIPPDPPALDTTPANHAPPSPLPGSRKRRRPVLPSSSESEGKPDIESESSSTESSEDEVGDLRGGRRRPPRELGGRYFLDLSAESTTGTESEGTGPSDDDDDDASDGWLVDTPPRKSKRPRINLRLTSSPDRRAGVVFPEVWRSDRPIRAAQPQAPAQSSGDRAHAPRRSARQAQMRSGAAWTLDLHYIRQCVNQ",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design must be focused on enhancing the efficiency of regulation of gene expression. The designed protein sequence is ",
    "response#1": "RGYDLASQVLNICNVHHKKLFCHITYRHLLVLSSDDNGCVILKKVITIADDFLKDEFLDLIAQHAHSLSMHDLGISLIQHVLELDFTKKTTQDDKRLHELMAEFDEVLSTSVTADVDKLHKLASKLMLDSDLFFEFVITRRGSLMIQIILGKSEEVDQVILAGVKQRFIDVTTNFYGYRIMIQTIKVFKKRGDLKVYDQILRLIGVHALYLTKDPDMGNKTFQHAINLHHQDCTTFIACGLQSHYIELSFLKHGSKIVEMLIDDRISMVPLVLLMMEIVKCDEDTLVRLATDEYGNNILKKFLALAKEHKEDFFGDLVDKLNPLLDSLRGTLGENIVAIIDSEC",
    "response#2": "RGYDLASQVLNICNVHHKKLFCHITYRHLLVLSSDDNGCVILKKVITIADDFLKDEFLDLIAQHAHSLSMHDLGISLIQHVLELDFTKKTTQDDKRLHELMAEFDEVLSTSVTADVDKLHKLASKLMLDSDLFFEFVITRRGSLMIQIILGKSEEVDQVILAGVKQRFIDVTTNFYGYRIMIQTIKVFKKRGDLKVYDQILRLIGVHALYLTKDPDMGNKTFQHAINLHHQDCTTFIACGLQSHYIELSFLKHGSKIVEMLIDDRISMVPLVLLMMEIVKCDEDTLVRLATDEYGNNILKKFLALAKEHKEDFFGDLVDKLNPLLDSLRGTLGENIVAIIDSEE",
    "response#3": "RGYDLASQVLNICNVHHKKLFCHITYRHLLVLSSDDNGCVILKKVITIADDFLKDEFLDLIAQHAHSLSMHDLGISLIQHVLELDFTKKTTQDDKRLHELMAEFDEVLSTSVTADVDKLHKLASKLMLDSDLFFEFVITRRGSLMIQIILGKSEEVDQVILAGVKQRFIDVTTNFYGYRIMIQTIKVFKKRGDLKVYDQILRLIGVHALYLTKDPDMGNKTFQHAINLHHQDCTTFIACGLQSHYIELSFLKHGSKIVEMLIDDRISMVPLVLLMMEIVKCDEDTLVRLATDEYGNNILKKFLALAKEHKEDFFGDLVDKLNPLLDSLRGTLGENIVAIIDSEC"
  },
  {
    "reference": "MKLGIVILAAGQGTRMKSSLPKVLHPLAGRPLLAHVLETARSLQPAKTVVVYGHGGERVRDTIDNPDILWVEQAEQLGTGHAVQQAMPLLQDMDKVLVLYGDVPLIRAESLQALVHEAASGLGLMTVTLDDPSGYGRIERDADGRVLRIVEQKDASEEQLAIPEINTGIMLIDTARLQGWLQELSPQNAQGEYYLTDIIAMAVRDGVDIHVTQPADTVEAEGINDCLQLASLERVLQRWQAEALMRDGVTLKDPARFDLRGSLSAGTDVVLDVNVIIEGQVVLGSNVTIGANTVLRNVQVADNVCIRENCVIEDAVIGPDSAIGPFSRIRPGTELVGGAHVGNFVEIKNSTIGLGSKVNHLSYIGDTQMGSGVNIGAGTITCNYDGAYKHQTVIEDKSFIGSNAALVAPVRIGENATIGAGSVIAKDAEAGELTLARGKQVTIRGWKRPSKEK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a high affinity for Mg(2+), acetyl-CoA, UDP-N-acetyl-alpha-D-glucosamine.2. The designed protein should be involved in lipid A biosynthetic process, regulation of cell shape, cell wall organization, UDP-N-acetylglucosamine biosynthetic process, peptidoglycan biosynthetic process by localizing to cytoplasm and exhibiting glucosamine-1-phosphate N-acetyltransferase activity, magnesium ion binding.3. The protein design should consider the endogenous proteins and enzymes present in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, Nucleotide-sugar biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLTATVPDPSGYGRLIRTSDGEVQRIVEQKDASEAERAVSFCNSGIMGFRAEALREVLVQLGNDNAQAEYYLTDIVEIARRDGLRAVAVVAPPEEVAGINTRAELAGAEAEFQRRLRLELLEAAGVMLMDPARIDVRGELICGADVEIDVNCIFEGTVKLGNRVKVKANCILKDVTVSEGSTIHPFSHVEEAKIGQNCKIGPYARIRPETQLIDNVHIGNFVEVKKSQIASGSKVNHLSYIGDTIIGQNVNIGAGTITCNYDGAYKHQTIIEDNVFVGSDTQLVAPVKVSYGSTIGAGSTITRDTPENELTLSRSKQVNKPGLAIRLMQRLRDKKS",
    "response#2": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLSATPPDPRGFGRLVREDGTVRRIVEQKDATPEERAIREVNTGILVAPTALLRGWLPRLSNENAQKEYYLTDIVALAVADGVPVTSVQPESAAETLGVNSPSQLAELERVFQRRQAEALMAAGVTVVDPASTWVDVSVEFEADAVVHPGTQLLGATRISTGAEVGPHSRITDTVVGAGAVASFTVAEGARIGDGANVGPYAYLRPGTDLGAKSKAGTYVEMKNASIGEGTKVPHLSYVGDATIGEYTNIGAASVFVNYDGEAKHHTTVGSHCKTGSDNMFVAPVTIGDGAYTAAGSVITKDVPPGSLAVARGQQRNIAGWVRPKKK",
    "response#3": "MILAAGKSKRMKSKTGKFLHPVLGKPIIRYVVEMSELLGSEKTVVIVNNESEPVKQALRDKELVYALQDPQLGTGHAVMCGVDQLEGGSTLILLGDAPCIREESLLDLFEAHEKAEADLTVLSVLLENPTGYGRIVRNERGEVISIVEQKDASPEEKNINEINTGIMVAPTKHLKSWLSQVKNDNAQKEYYLTDIIAMAVQDGVKIVTAQPEYEFEAMGVNSKSQLAELERIHQRNIADSLLEQGVSLIAPETVWFSSDTQVSSDVIIEPNVIFGPGVSIASGATIYGHSHIEGATIGANAKVGPFARLRPGAVMEEGSKVGNFVEMKKATLGKGAKANHLTYLGDAEVGEGANIGAGTITCNYDGYFKYKTVIGKGAFIGSNSALVAPVNIGDGAIVGAGATLTKDVAAGDLALVRAEQRHISGWQRPKKDS"
  },
  {
    "reference": "MANTNFSPGLNQALALDQQDPLRKFRDRFYQLEGGKIYMDGNSLGLASKDAEDALLRVLEVWKKEGILMWNIDDGYFFQYARILGNRLAKLINAGENEVIVAGNTTVNIHQLIATFWQPTKERYKILVDDLNFPTDRYAIDSQIRLKGMQVEDVLKIVKSKDGIFIDEDEVIDAMTDDVAVVLLPSVLYRSAQLLDMERLTAEAHKRGILIGFDLCHSIGCVVHDFQKIDADFATWCNYKYISAGPGASAGLYVNKRHFGKPVGLAGWFGNRDETQFQLKHTFDQAPDAIAWQTGTPNHFSMAPIEGVLNIFEEAGLQHIHEKSLNLTAYLMYLIDQNLKPFGFSYNNPTDDTKRGGHVSLVHEEAYRICLALKQHQVVPDFREPNVIRLAPIALYNSYEDAFKIVEVLKQIGEQKEWEQYSAVRAMVT",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein requires pyridoxal 5'-phosphate to activate a critical catalytic site.2. The protein should be designed to have high stability and activity towards H2O + L-kynurenine = anthranilate + H(+) + L-alanine.3. The protein design should take into account the physiological conditions of the quinolinate from L-kynurenine: step 2/3, Cofactor biosynthesis, NAD(+) biosynthesis pathway.4. The protein must exhibit the following characteristics: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively..5. The designed protein should be involved in L-kynurenine catabolic process, tryptophan catabolic process, 'de novo' NAD biosynthetic process from tryptophan, quinolinate biosynthetic process by localizing to cytoplasm and exhibiting pyridoxal phosphate binding, kynureninase activity.6. The pyridoxal 5'-phosphate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MSDHLRTEDIVSKLAEIGDEVAVVWIGCVQYYTGQFFEVEPIARKTHEIGAYFGLDLAHAIGNVPVKLNDWGVDFAAWCTYKYLCGGPGSVAGYYIKDGLDDGGRRLAGWWGHNRQTRFHMSPEFDPIPGAGGYQHSNPPVFSSLPLIATLEIIDKAGGIDVLREKSERLTGYFWDLLTSSKWYRGIASPDRQERLAAYRREDHLRTEDIVSKLAEIGDEVAVVWIGCVQYYTGQFFEVEPIARKTHEIGAYFGLDLAHAIGNVPVKLNDWGVDFAAWCTYKYLCGGPGSVAGYYIKDGLDDGGRRLAGWWGHNRQTRFHMSPEFDPIPGAGGYQHSNPPVFSSLPLIATLEIIDKAGGIDVLREKSERLTGYFWDLLTSSKWYRGIASPDRQERLAAYRREAEDDHLRTEDIVSKLAEIGDEVAVVWIGCVQYYTGQFFEVEPIARKTHEIGAYFGLDLAHAIGNVPVKLNDWGVDFAAWCTYKYLCGGPGSVAGYYIKDGLDDGGRRLAGWWGHNRQTRFHMSPEFDPIPGAGGYQHSNPPVFSSLPLIATLEIIDKAGGIDVLREKSERLTGYFWDLLTSSKWYRGIASPDRQERLAAYRREAEDDYQERFAEPLSRIVGAKPSEVTVMNTLTVNLHLLMVSFYQPTKTRYKIICEEKAFPSDQYMFQSQVAFHGYKKEETIIEIKRREGEHNIRLEDILAKINEVGDELALVLIGGVNYYTGQVFDMKTITEAGHKVGAKVGFDLAHAAGNIKLDLHDWNVDFAAWCSYKY",
    "response#2": "DHLRTEDIVSKLAEIGDEVAVVWIGCVQYYTGQFFEVEPIARKTHEIGAYFGLDLAHAIGNVPVKLNDWGVDFAAWCTYKYLCGGPGSVAGYYIKDGLDDGGRRLAGWWGHNRQTRFHMSPEFDPIPGAGGYQHSNPPVFSSLPLIATLEIIDKAGGIDVLREKSERLTGYFWDLLTSSKWYRGIASPDRQERIEAFLAGDHLRTEDIVSKLAEIGDEVAVVWIGCVQYYTGQFFEVEPIARKTHEIGAYFGLDLAHAIGNVPVKLNDWGVDFAAWCTYKYLCGGPGSVAGYYIKDGLDDGGRRLAGWWGHNRQTRFHMSPEFDPIPGAGGYQHSNPPVFSSLPLIATLEIIDKAGGIDVLREKSERLTGYFWDLLTSSKWYRGIASPDRQERIEAFLAGWRAQP",
    "response#3": "MFDHLRTEDIVSKLAEIGDEVAVVWIGCVQYYTGQFFEVEPIARKTHEIGAYFGLDLAHAIGNVPVKLNDWGVDFAAWCTYKYLCGGPGSVAGYYIKDGLDDGGRRLAGWWGHNRQTRFHMSPEFDPIPGAGGYQHSNPPVFSSLPLIATLEIIDKAGGIDVLREKSERLTGYFWDLLTSSKWYRGIASPDRQERLCQLLGGGDHLRTEDIVSKLAEIGDEVAVVWIGCVQYYTGQFFEVEPIARKTHEIGAYFGLDLAHAIGNVPVKLNDWGVDFAAWCTYKYLCGGPGSVAGYYIKDGLDDGGRRLAGWWGHNRQTRFHMSPEFDPIPGAGGYQHSNPPVFSSLPLIATLEIIDKAGGIDVLREKSERLTGYFWDLLTSSKWYRGIASPDRQERLCELLGGWPGGELTVERRKRSP"
  },
  {
    "reference": "MNFPDSSLFTPNFAYENDLDFSSLITPSTRVSFQEPKPCNPVIHSAGIENDGRQNCETTMTLSEIMKGDDEPKNKRAKHKELERQRRQENTSLFKILRYLLPSQYIKGKRSSADHVLEAVNYIKDLQKKIKEVSEKRDRIKRSITHPSSRGEFSIRSLASSTCSCVGDTNIAVVVRPCLIGLEIVVSCCNRHESCLSSVLQLLAQEQCFNIVSCISTRLHQGFIHTIASEVEEGIEVYFSELQEKIIKIGTSRVTTR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must have at least one bHLH domain.2. The protein should have DNA-binding transcription factor activity, DNA-binding transcription factor activity, RNA polymerase II-specific, RNA polymerase II transcription regulatory region sequence-specific DNA binding and be active in RNA polymerase II transcription regulator complex. The designed protein sequence is ",
    "response#1": "FLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFR",
    "response#2": "LHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRR",
    "response#3": "FLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFV"
  },
  {
    "reference": "MILSTIEEALEDIRAGKMIILVDDEDRENEGDLTIAADHVSPEAINFMARHGRGLICLSITEERARQLQLPPMVQDNSSPFGTAFTVSIEARKGVTTGISAHDRSTTIRVAIAPSARPDDLVRPGHVFPVVARKGGVLVRTGQTEGSVDLARLAGCSPAGVICEVMNEDGTMARVPDLRRFAKEHGLKIASIRDLVEYRMHRERLVKRVGETEMPIRTGGEFTAIAYRNTMNGDTHIAMVHGEIRSDVPVLVRVHSECLTGDVFGSVRCDCGEQLRNALQKIQTEGSGILLYMRQEGRGIGLENKIRAYRLQDKGFDTVEANQRLGFKPDLRDYGVGAQILVDLGVRDIRLLTNNPKKIIGLEGYGLRVVDRVAVEVSPNDSNVKYLKTKKKKMGHILSVD",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must have at least one GTP cyclohydrolase II domain.2. The designed protein must have a Nucleophile; for GTP cyclohydrolase activity that is structurally stable and resistant to denaturation.3. The designed protein should have a binding site that is specific to the target GTP, Zn(2+) ligand.4. The designed protein should be optimized for zinc ion binding activity in order to improve riboflavin biosynthetic process. The designed protein sequence is ",
    "response#1": "VGTAKLPSRYGEFVVHAYESVLDGKGHLALVKGDLSSVEAPLVRVHSECLTGDVFGSLRCDCGNQIGRALRQIEAEGVGVLLYMRQEGRGIGILNKIRAYELQDEGKDTVEANEVLGFPPDLRDYGIGAQILSDLGLTKLRIMTNNPRKIAGLEGHGLKVVERR",
    "response#2": "VGTAKLPSRYGEFVVHAYESVLDGKGHLALVKGDLSSVEAPLVRVHSECLTGDVFGSLRCDCGNQIGRALRQIEAEGVGVLLYMRQEGRGIGILNKIRAYELQDEGKDTVEANEVLGFPPDLRDYGIGAQILSDLGLTKLRIMTNNPRKIAGLEGHGLKVVERR",
    "response#3": "SVADLPTEFGHFHIYGYHNSLDDSDHVAIVKGDPANFDHGQVMVRMHSECLTGDALGSLRCDCRMQLQAALKMIEAAGQGVVVYLRQEGRGIGLINKLKAYSLQDMGLDTVEANEKLGFPADLRNYGVGAQILNDLGIKKIRLITNNPRKIAGLKGYGLEVVDRLR"
  },
  {
    "reference": "MMYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLSEKPEIKQKWASRLLAKLRKDIRQEYREDFVLTVTGKKHPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLMKSPHCTNPALCVQPHHITVSVKELDLFLAYYVQEQDSGQSGSPSHNDPAKNPPGYLEDSFVKSGVFNVSELVRVSRTPITQGTGVNFPIGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDENMEPSPTGDFYPSPNSPAAGSRTWHERDQDMSSPTTMKKPEKPLFSSTSPQDSSPRLSTFPQHHHPGIPGVAHSVISTRTPPPPSPLPFPTQAILPPAPSSYFSHPTIRYPPHLNPQDTLKNYVPSYDPSSPQTSQSWYLG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have sequence-specific double-stranded DNA binding to facilitate glial cell differentiation, forebrain development.2. The CTF/NF-I domain within the designed protein should exhibit high affinity and specificity towards its target DNA sequence.3. The designed protein must have at least one CTF/NF-I domain. The designed protein sequence is ",
    "response#1": "MGSRLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKAEVKQKWASRLLAKLRKDIRPECREDFVLAITGKKAPGCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKAAQCGHPVLCVQPHHIGVAVKELDLYLAYFVRERGEAGRARR",
    "response#2": "LMDSDVKDNQYRLMLGKEPVKKMMDALGKTEKLGTKGLNVSVYGPNGENHKMVLKIWIKGTPVLTSGWKNFVKSYKLEKHVDFLTIWMFK",
    "response#3": "QERKCFSVKYASKYLPHKDQNMRLRLPETKYKCKAALHIDTSTNLHKLLKGWGKFVNDNKLEIHDICLFQLMKNKKKLTMTVHIIV"
  },
  {
    "reference": "MGXKWSKXSIVGWPDVRERIRXAAPAAEGVGAASQDLAKYGALTTNNTPATNADCAWLQAQEEDEVGFPVRPQVPLRPMTYKAAFDLGFFLKEKGGLEGLIYSKKRQEILDLWVYHTQGXFPDXQNYTXGPGVRYPLTFGWCFKLVPVDPEEVEKANEGENNCLLHPXSLHGMEDEEKEVLKWQFDISLAHRHVARELHPEYYKD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should include a PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Diacidic; necessary for CD4 internalization motif to ensure proper function.2. The designed protein should have SH3 domain binding within membrane, extracellular region to regulate suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II.3. The protein must exhibit the following characteristics: Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis. The designed protein sequence is ",
    "response#1": "MGGKWSKRTGKGWATVRERMRRTEPAADGVGAASRDLEKHGAITSSNTAATNAACAWLEAQEEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPDQVEEANEGENNCLLHPMSQHGMDDPEREVLQWKFDSRLAFHHVARELHPEYYKNC",
    "response#2": "MGGKWSKSSIVGWPAVRERIRRTEPAAEGVGAASRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSRKRQEILDLWIYHTQGYPVXPMTYXAAFDLSFFLKEKGGLDGLIXSQERQDILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPSEVEEANTGENNCLLHPMSQHGXDDPEREVLMWKFDSRLAFXHVAREKHPEYYKNC",
    "response#3": "MGGKWSKRSKREWPDVREKIEGPAAEGVGAVSRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLVWSQKRQDILDLWVYHTQGFFPLAPGGPDRKGTEXNQEETEEVGFPVRPQVPLRPMTFKGALDLSHFLKEKGGLDGLVYSQKRQEILDLWVYHTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPEKVEEANAGENNALLHPICQHGMDDADREVLMWKFDSRLAFTHKARELHPEFYKDC"
  },
  {
    "reference": "MKKVSVIAAAVAATLAAGSAFAVDFNGYFRAGTGISGNGNADQAVNKAGTGRLGNENDNYYEFGFAEELKTGEQTWKVESMIAQGNSGANGWEDGDFNVAQFNVQAKGLLASDQEAVMWAGKRYYQRKDIHITDFYFLNTSGTGGGIENLSVGNQKLSVALVQDGDNTNSSGYIFDARLANIGLWENASLELAMAYNFATEKDSKNEVADDGVLVSAILHQGLSNGFNQTVFQYGTAGYGAQAANFWGAGSYYARGTEAFNDASGFRLLNWGVINLGENWEMGHQLAYLAGSDIGGQFGGDGANKNTYTGKSFDIDQYSVVVRPMYKWNDTMRTVFEAGYNAGEKISNGGLATEDFGNAKFTVAQAWAMGDSFWARPELRVYGTYLLDTENDKAFGDDDTEFVVGIQVEAWW",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. We need a protein that can perform beta-maltose(in) = beta-maltose(out) with high efficiency.2. The designed protein should have maltose transporting porin activity, carbohydrate transmembrane transporter activity, porin activity, maltodextrin transmembrane transporter activity within pore complex, cell outer membrane to regulate polysaccharide transport. The designed protein sequence is ",
    "response#1": "LWNSVKVAGISAIGIVALSTTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHGGVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVVFILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPF",
    "response#2": "MRTANETLSFAPFLPKEWDEYSFNINYRNRLINVTVDEKRVIFELVKGEPLHMNVYEEPVVL",
    "response#3": "MRTANETLSFAPFLPKEWDEYSFNINYRNRLINVTVDEKRVIFELVKGEPLHMNVYEEPVVL"
  },
  {
    "reference": "MWAYKYLFKSLFTFRWACWVMVILFVRGIAKLFCTAAAPFHIPTSNAQVFHFLHILANSSNNSCHSLLFNLIVEYEKEFSPGQRHHKEFKFNLSQIPEGEAVTAAEFRIYKDCVVGSFKNQTFLISIYQVLQEHQHRDSDLFLLDTRVVWASEEGWLEFDITATSNLWVVTPQHNMGLQLSVVTRDGLTINPRAAGLVGRDGPYDKQPFMVAFFKVSEVHVRTTRSAPGRRRQQSRNRSTQSQDVSRVSSASDYNSSELKTACRKHELYVSFQDLGWQDWIIAPKGYAANYCDGECSFPLNAHMNATNHAIVQTLVHLMNPEYVPKPCCAPTKLNAISVLYFDDNSNVILKKYRNMVVRACGCH",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should exhibit Polar residues compositional bias.2. The designed protein must possess growth factor activity, protein heterodimerization activity to support positive regulation of transcription by RNA polymerase II under certain conditions.3. The protein contains novel TGF-beta family profile domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "IELDMLVGKGRFAQVYKAKLKQTTSDQFETVAVKIFPYEEYASWKNEKDIFSNTDLRHENILHFLTAEDRKVEKQYWLITAFHPQGNLQEYLTRHVTSWEELQVLSSSLARGVAHLHSDRLPCGRPKVPIVHRDLKSSNILVKNDLTCCLCDFGLGLCLDSSLSVDDLANSGQVGTARYMAPEVLEARLNLENTESFKQTDIYSMALVLWEMTSRCEAIGEVKDYEPAFGSKVREHPCVESMKDNVLRDRGRPEIPDSWLRHQGVAVICATIKECWDHDPEARLTAHCVAERII",
    "response#2": "DSSRMPSVGDYNTSEQKQACKKHELYVSFRDLGWQDWIIAPEGYAAFYCDGECSFPLNAHMNATNHAIVQTLVHLMFPDHVPKPCCAPTKLNAISVLYFDDSSNVILKKYRNMVVRSCGCS",
    "response#3": "AFRVSNLGENTSSDQRQACKKHELYVSFRDLGWQDWIIAPEGYAAYYCEGECAFPLNSYMNATNHAIVQTLVHFINPNTVPKPCCAPTQLHAISVLYFDDSSNVILKKYRNMVVRACGCR"
  },
  {
    "reference": "MIQNTSVKYIFVLGGVISGLGKGIAAASIGYLLKSSGLSVTILKLDPYLNVDPGTMNPYQHGEVFVLDDGSETDLDLGHYERFIDENMSKINNATTGQIYSRVLERERAGDYLGKTVQVIPHITDEIKHRIKKVNTPKRFDVVICEVGGTVGDIESLPFLEAIRQLSVEVGYRNHQIVHVTLLPYIKASEELKTKPTQHSVMKLREIGLAPDIILCRSEYKLTKSIRDKIALFCNVLPAHVVEGKEVKNIYEVPLIFHQQKLEELIAARLQLPYSPNVSKLEQFIYRYKHPKTNVTIAMCGKYTELPDAYKSVLEAFVHAGVENDAQVKVKWISTEDMRTDEDAREGFNNIDGILLLPGFGSRGSEGKILACKYARENQIPFLGICLGLQCAVIDFARHVCGLKGANSTEFNRGTPYPVIKLLESQRAVKAKGGTMRLGAYDCEISSGTRAYTAYKKKKISERHRHRWEVNNRYRTRFEKSGMVISGVNSDLNLVEIIELSNHPWFVTCQFHPELKSRILKAHPLFREFIKATVVYTGGKR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must have a Nucleophile, active site that is structurally stable and resistant to denaturation.2. The protein should be optimized for activity in CTP from UDP: step 2/2 pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The protein must have high specificity towards CTP synthase activity. The designed protein sequence is ",
    "response#1": "LPDAYLSIVEALRHAGYGVDAEIDVRWVNAEDVTDENAAELLGKADGIIVPGGFGDRGVEGKISAIRYARENNVPFFGICLGMQLAAVEFARNVVGLKGANSSEIDPETKYPVIDLLPEQKDVEELGGTLRLGLYDCHLVEGTHAANAYGTDLVAERHRHRYEFNHDFREQLEQAGLIISGTSPDGRLVEIIELKEHRYFVAAQFHPEFTSRPTRPQPLFLEFL",
    "response#2": "LPDAYKSIIESLNHAAVYNDRKLELELIHSQTLNEGNVATKLKGMDGLIVAPGFGNRGIEGKFTALKYGREHNLPTFGICLGMQCMVIEFARNVLGFVEANSTEMDPNTTHNVIDIMEDQKSVSDYGGTMRLGNYTCKLNKSSKAYKAYGREIIQERHRHRFEFNDSFKQLYEQAGMSCSGINPDTNLVEVIEIPALTWYIGCQFHPEYNSTVLNPNPLFVDFVRSYGG",
    "response#3": "LPDAYLSVSEAVRAAGFAHRARIQLRWVPSDECVSPTGAAAALSGVDGVVIPGGFGVRGIEGKIGAARYAREQGIPILGLCLGLQCMTIEVARHLAGLSGANSREFDEQAAHPVIATMADQADIVAGRGDLGGTMRLGAYPAVLTPDSLVAKAYEATEVSERHRHRYEVNNAYRDRLTQAGLRISGTSPDGRLVEFIELDAGLHPFFVATQAHPELKSRPTRPHPLFAAFVK"
  },
  {
    "reference": "MQDQLVCHGCRNLLMYPRGASNVRCALCNTINMVPPPPPPHDMAHIICGGCRTMLMYTRGASSVRCSCCQTTNLVPESSFTLLFDNILKVLKTKLLDGPGGLAHSNQVAHAPSSQVAQINCGHCRTTLMYPYGASSVKCAVCQFVTNVNMSNGRVPLPTNRPNGTACPPSTSTSTPPSQTQTVVVENPMSVDESGKLVSNVVVGVTTDKK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with DNA-binding transcription factor activity is needed to enhance response to superoxide, aerenchyma formation, regulation of hydrogen peroxide metabolic process in a specific context.2. The designed protein have Zinc finger LSD1-type domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "FQWQDPTPKGKKISSATANSIKKAALKSGLSVSELVKVAWASASTFRSTDMRGGANGARIALAPQKSWEVNNPKLLEKALPKLEKISKRYGASLADTIVLGGAAAIEKAARDAGVKVKVPFKSGRGDATQEQTDITSFKFLEPKADAFRNYYTEESGRTPIYMLIDKANMLGLTVPEMTVLVGGMRVLDANADGSKHGVFTKRPGRLSNDFFVNLLTMDNKWVKSSMPGIYEGRDRKTNELKYTATPVDLVFGSHAELRAVAEVYASL",
    "response#2": "FQWQDPTPKGKKISSATANSIKKAALKSGLSVSELVKVAWASASTFRSTDMRGGANGARIALAPQKSWEVNNPKLLEKALPKLEKISKRYGASLADTIVLGGAAAIEKAARDAGVKVKVPFKSGRGDATQEQTDITSFKFLEPKADAFRNYYTEESGRTPIYMLIDKANMLGLTVPEMTVLVGGMRVLDANADGSKHGVFTKRPGRLSNDFFVNLLTMDNKWVKSSMPGIYEGRDRKTNELKYTATPVDLVFGSHAELRAVAEVYASC",
    "response#3": "FQWQDPTPKGKKISSATANSIKKAALKSGLSVSELVKVAWASASTFRSTDMRGGANGARIALAPQKSWEVNNPKLLEKALPKLEKISKRYGASLADTIVLGGAAAIEKAARDAGVKVKVPFKSGRGDATQEQTDITSFKFLEPKADAFRNYYTEESGRTPIYMLIDKANMLGLTVPEMTVLVGGMRVLDANADGSKHGVFTKRPGRLSNDFFVNLLTMDNKWVKSSMPGIYEGRDRKTNELKYTATPVDLVFGSHAELRAVAEVYASC"
  },
  {
    "reference": "MDIAIHHPFSRRPLFSFLTPTRIFDQSFGEHLLESELFPRATDVISSLVQKPVPKTISILDGGILFKKMRYLLHLKRAVKNKNSSKTMRLEKDKFSVNVDVKHFSPEELKVKVLGDVIEVHGKHEERQDEHGFVAREFNRKYRIPADVDPLSITSSLSSDGVLTVNGPRKQSDVPERTIPITREEKSAIAGAQRK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.2. The protein contains novel SHSP domains that confer a unique function or activity.3. The designed protein should have protein-containing complex binding, protein homodimerization activity within nucleus, Z disc, mitochondrion, cytosol to regulate tubulin complex assembly, response to hydrogen peroxide, apoptotic process involved in morphogenesis. The designed protein sequence is ",
    "response#1": "KLGETLGLGSTGKVQLARNGSTGQEAAVKVISKAVFNTGNVSGTSIVGSTTPDALPYGIEREIIIMKLLNHPNVLRLYDVWETNTDLYLVLEYAEKGELFNLLVERGPLPEHEAIRFFRQIIIGVSYCHALGIVHRDLKPENLLLDHKYNIKIADFGMAALETEGKLLETSCGSPHYAAPEIVSGIPYQGFASDVWSCGVILFALLTGRLPFDEEDGNIRTLLLKVQKGEFEMPSDDEISREAQDLIRKILTVDPERRIKTRDILKHPA",
    "response#2": "DSEKFEVILDVQQFSPSEITVKVADKFVIVEGKHEEKQDEHGYVSRQFSRRYQLPSDVNPDTVTSSLSSDGLLTIKAPMKALPPPQTERLVQIC",
    "response#3": "HAGNPKNNPVKVRSDRDKFVIFLDVKHFSPEDLTVKVLEDFVEIHGKHNERQDDHGYISREFHRRYRLPSNVDQSALSCSLSADGMLTFSGPKVQSGLDAGHSERAIPVI"
  },
  {
    "reference": "EELFCRTMHGVIKNIAHLCKRDRSKTWGKEGWKKVVVCIVSDGRQKINSRTLSVIAAMGRHQDGIAKNVVNKKPVTAHIYEYTTQITVTPSMKIEGAERGTMPVQLIFCLKEKNQKKINSHRWFFNAFGPILQPNVCVLLDVGTMPGPTSIYHLWKAFDINSNVGGACGEIVALKGKWGLNLLNPLVAA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should be able to enhance the efficiency of chitin biosynthetic process.2. For general function, the protein need meet that Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non- reducing end of the growing chitin polymer. The designed protein sequence is ",
    "response#1": "PLESVFGYITVLPGALSAYRYHALQNDSTGHGPLSMYFKGEMLHGKNADVFTANMYLAEDRILCWELVAKREEQWVLKFVKSAVGETDVPDTVPEFISQRRRWLNGAFFAAVYSLIHFRQIWRTDHTITRKILLHIEFLYQFVSLAFTFFSLANFYLTFL",
    "response#2": "IAVYYGQGVNQPRLAEFCAETSYDIINIGFINSFPEQNPLTGLPGSDFGNQCWADTFVVDGIASQLYSHCPNIAEDIPKCQAAGKKVFLSLGGATPTYWFDTIDASTKLADFLWGAFGPVTDAWTVADKPRPFGNAVVDGFDFDIEFFGSKGYANMIKRFRRRFGEVPDQTFYISAAPQCSIPDEQLSVAIKNAVIDFVWVQFYNTPGCSARDFVLGTKNGFNYDSWVEVIKAGANPNAKLYVGLPASGAAALM",
    "response#3": "PLESVFGYITVLPGALSAYRYHALQNDSTGHGPLSMYFKGEMLHGKNADVFTANMYLAEDRILCWELVAKREEQWVLKFVKSAVGETDVPDTVPEFISQRRRWLNGAFFAAVYSLIHFRQIWRTDHTITRKILLHIEFLYQFVSLAFTFFSLANFYLTFL"
  },
  {
    "reference": "MPENELQNDFFRYLIVERGYSDKTKSAYQEDMEDFFRFLKESGNEDFLAIDHRDVRVYLSFLNERNYSRNSISRKMASLRSFYHFLTKHEVIKENPFAYIHLKKKNAKLPRFFYEKEMEALFESVKGHEPLQQRNRALLEILYGSGLRVSECSNLTLSDVDWENGVLLIHGKGNKDRYVPFGSYAQEALRDYVSAGRQQLMEKHHKRHEIVFVNHLGDGITATGIEYVLNQIIRKSSLDSKIHPHMLRHTFATHLLNHGADMRTVQELLGHANLSTTQIYAHVTKDSLQKITANFIQELKNWRQEERIMKSPFHSTTICAVEKDGKFAMAGDGQVTMGEQVVMKGTARKVRRIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEAMLIVMNQEEMLLVSGTGEVISPDEGILAIGSGGNYAIAAARAMKRYGGDLSAKEIAEGALNIAADICVFTNHNIIVEEF",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein have Core-binding (CB) domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "AYSSSLQPRLRALKGFLRENLYWHYQVLRMTDKARRIIGDLFGAFMADPRLLPPQYQEKARTDKPRAIADYIAGMTDRYAMKEHRR",
    "response#2": "AYSSSLQPRLRALKGFLRENLYWHYQVLRMTDKARRIIGDLFGAFMADPRLLPPQYQEKARTDKPRAIADYIAGMTDRYAMKEHRR",
    "response#3": "AYSSSLQPRLRALKGFLRENLYWHYQVLRMTDKARRIIGDLFGAFMADPRLLPPQYQEKARTDKPRAIADYIAGMTDRYAMKEHRR"
  },
  {
    "reference": "MLYQQIAKNKRKTVLVMAGFVFLAGLIGAAIGYAFMGSAQTGIIIAVVVGIVYMFVILGQSTDVVMSMNNAQEITEQQAPELWHIVEDMAMVGKVPMPRVFIIDDPSPNAFATGPDPEHAAVAATTGILQRLNREELEGVMAHEVSHIRNYDIRLQTYALALASVISFLANMGMNAMWWGGGRRDDRDNGNAGQVIMLLLSVLAIILGPLAASMAQMALSRNREYLADASAVELTRNPQGLINALRKISMSEPMQNADPSSASMYIANPFKDGSWTHLFDTHPPIEKRIDRLEHM",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.2. The designed protein should be expressed specifically in the plasma membrane3. The Zn(2+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MMAWMNAESVAKTLETGRVTYWSRSRQSFWIKGESSGHVQELVELRVDCDRDALLAMVRQTGPACHTNRRSCFI",
    "response#2": "MMAWMNAESVAKTLETGRVTYWSRSRQSFWIKGESSGHVQELVELRVDCDRDALLAMVRQTGPACHTNRRSCFR",
    "response#3": "MMAWMNAESVAKTLETGRVTYWSRSRQSFWIKGESSGHVQELVELRVDCDRDALLAMVRQTGPACHTNRRSCFS"
  },
  {
    "reference": "MNQDKRAFMFISPHFDDVILSCASTLMELMNQGHTCKVLTVFGGCPSVRFQPGEIARQYAAEDLGLFEDEIEGDHLSILVARRLQEDQQAFRHLPGVQVEVLSFPDAIYRENKGQPYYRTEADLFGIPDKQDEDIFLPKIETYLQSCDLARKYTWVFPAISKHVDHRLLTKAGLRLMSQGYPVLFYSEFPYWQQHNEFLQDGWRQLELRNSVYTPVKRAAVLEYKTQLLGLFGEEAETKINNGGVLSEAELFWIQETDTQAWRVFRSLSPEPLQT",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. Zn(2+) is required for the proper folding and stability of the protein.2. We need a protein that can perform 2'-N-acetylparomamine + H2O = acetate + paromamine with high efficiency.3. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "ANIDQMVIVSSVLPELSLNIIDRYLIAAETLGIEPLLVLNKIDLLQEAELATYREWLADYEKIGYKILYVSKRSGAGIAELEAQLQDRINIFVGQSGVGKSSLVNALIPELDIEEGEISELSGLGQHTTTAARLYHIPSGGNLIDSPGVHGLTVTEYVISCYGARKGLVDTALRTADSGYLTRRLVDVAQDMIIRETDCGTKSGILLGPVKDEQKVVFSLQERLIGRVLAENIYSPKQKQYIAKKNQDISASLSDKICEIKRTNILVRSPLTCKSIRSVCQLCYGWSLAHGNLVDLGEAVGIIAAQSIGEPGTQLTMRTFHTGGVFTGNIAKQIRSSIDGKIIYSLTNTIPMRTRHGEIALITQSNINICIQGKNKEFNIEVPINTILLVNNGSMVRAKQLIAEVSAPVNAQSVEIASKYVASDFSGEIHFENLLLQEKKSAYGHKMNRAGQYGGLLWILSGQVYKFSLDVPLMFDTADYIHNGSCMAEHNIISQYGGILQTSENNLTIDNSNSYLYVLNSSMTIINAYLSHIKNARSEAYILTDDLNQKFFMQVESNNVIKNNQIIAKFTSVLTRPDYYDGLKPVAANIDQMVIVSSVLPELSLNIIDRYLIAAETLGIEPLLVLNKIDLLQEAELATYREWLADYEKIGYKILYVSKRSGAGIAELEAQLQDRINIFVGQSGVGKSSLVNALIPELDIEEGEISELSGLGQHTTTAARLYHIPSGGNLIDSPGVREGLTVTEYVISCYGARKGLVDTALRTADSGYLTRRLVDVAQDMIIRETDCGTKSGILLGPVKDEQKVVFSLQERLIGRVLAENIYSPKQKQYIAKKNQDISASLSDKICEIKRTNILVRSPLTCKSIRSVCQLCYGWSLAHGNLVDLGEAVGIIAAQSIGEPGTQLTMRTFHTGGVFTGNIAKQIRSSIDGKIIYSLTNTIPMRTRHGEIALITQSNINICIQGKNKEFNIEVPINTILLVNNGSMVRAKQLIAEVSAPVNAQSVEIASKYVASDFSGEIHFENLLLQEKKSAYGHKMNRAGQYGGLLWILSGQVYKFSLDVPLMFDTADYIHNGSCMAEHNIISQYGGILQTSENNLTIDNSNSYLYVLNSSMTIINAYLSHIKNARSEAYILTDDLNQKFFMQVESNNVIKNNQIIAKF",
    "response#2": "VEPRTSVLTRPDYYDGLKPVAANIDQMVIVSSVLPELSLNIIDRYLIAAETLGIEPLLVLNKIDLLQEAELATYREWLADYEKIGYKILYVSKRSGAGIAELEAQLQDRINIFVGQSGVGKSSLVNALIPELDIEEGEISELSGLGQHTTTAARLYHIPSGGNLIDSPGVREFGLE",
    "response#3": "TSVLTRPDYYDGLKPVAANIDQMVIVSSVLPELSLNIIDRYLIAAETLGIEPLLVLNKIDLLQEAELATYREWLADYEKIGYKILYVSKRSGAGIAELEAQLQDRINIFVGQSGVGKSSLVNALIPELDIEEGEISELSGLGQHTTTAARLYHIPSGGNLIDSPGVRETSVLTRPDYYDGLKPVAANIDQMVIVSSVLPELSLNIIDRYLIAAETLGIEPLLVLNKIDLLQEAELATYREWLADYEKIGYKILYVSKRSGAGIAELEAQLQDRINIFVGQSGVGKSSLVNALIPELDIEEGEISELSGLGQHTTTAARLYHIPSGGNLIDSPGVRECLS"
  },
  {
    "reference": "MKAXGMKRNYQNLWGWGILLFWMIIMCXAGEDLWVTVYYGVPVWRDAETTLFCASDAKAYDKEVHNVWATHACVPTDPSPQELDLXNVTENFNMWKNNMVEQMQTDIISLWDQSLKPCVQLTPLCVTLDCTDPXINSTXSNSTALTPTNSTDMRNCTFNMTTELRDKKXKVXSLFYXLDIVPXGNISXNXSGXYRLINCNTSTMTQACPKVTFEPIPIHYCAPAGFAILKCKDPXFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEKXXIIRSENITDNSKNIIVQFNKTVXITCIRPGNNTRTSIRIGPGQXFYATGXXIGDIRKAYCXVXRXXWNSTLQQISAQLGKHFNKXIIFXDSSGGDLEVTTHSFNCGGEFFYCNTTELFNSXWINNGTVYNGTVXNITGGNXTITLPCRIKQIINMWQRVGXAMYAPPIXGNISCTSNITGLLLTRDGGXNXTNETFRPIGGDMRDNWRSELYKYKVVEIEPIGVAPTRAXRRVVERAKRAVGLGAVXFGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTSVRWNSTWSNKSYDEIWDNMTWMQWDKEVINYTNIIYXXIEDSQLQQEKNEQALLELDKWASLWNWFDISNWLWYIXIFIMIVGGLIGLRIVFAVLAIINRVRQGYSPLSFQTLTPHPEEPDRPXGIEEXGGEQGRDRSIRLVSGFLALAWDDLRSLCLFS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must contain a signal peptide for proper functionality.2. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.3. The protein should be optimized for stability and solubility when expressed in plasma membrane, virion membrane where it will be involved in positive regulation of receptor clustering. The designed protein sequence is ",
    "response#1": "MGAASLTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLQDQRLLGIWGCSGKLICTTTVPWNDSWSNRSYDSIWDNMTWMQWEKEIDNYTGVIYNLLEKSQNQQEKNEQELLELDKWASLWNWFDITKWLWYIKIFIMIIGGLIGLRIVCAVLSIVNRVRQGYSPLLFQTY",
    "response#2": "MGEIKNCSFKATTELKDKKRKEQALFYTLDIVSIDKVYDSCYRHFSMYRLIDCNTSAITQACPKVSFDPVPIHYCAPAGYAILKCNDKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIIRSENLANNVKTIIVQFNESVEIVCTRPNNNTRKSVRIGPGQTFYATGEIIGNIRQAHCNISESKWNKTLQNVAKRLKEHFPNKNITFAPASRGDLQITTPSFYCRGEFFYCYTSSLFNSTSNSHDAERDYNGAGNSSSDSFITILCRIKQFIIMWQRVGRAMYAPPIVGSVTCCSFITGLLLTRDGGSNTTETFRPVGGDMRDNWRSELYKYKVVEVRPLGIAPTRAQRRVVERVKR",
    "response#3": "MGAASVTLTVQARQLLSGIVQQQSNLLKAIEAQQQMLRLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKSQSEIWDNMTWLQWDKEINNYTNTIYNLLEEAQNQQEKNEQDLLTLDQWASLWNWFSISKWLWYIRIFIMIVGGLIGLRIVFAVLALINRVRQGYSPLSFQTL"
  },
  {
    "reference": "MRSRGSDMTDHGMNKNGASIPHSVWPADWLRNAEKAAADSFGLTLFELMQRAGDAAFQVARAAYPNRQRWLVLCGHGNNGGDGYVVARLAQAAGIDVTLLALESDKPLPEEAQAAREAWLDAGGEIHAPHTPLPETCDLIIDALLGTGLTQAHREAVADLIEQTNRHPAPVIALDIPSGLLAQTGGAPGAVIDAAHTVTFIALKPGLLTGRARDVVGQLHYHALGLEAWLAGQTAPYARLDETLLSHWLKPRRPGSHKGDNGKLVIIGGDHGTAGAIRMTGEAALRAGAGLVRVLTRVENIAPLITARPELMVHELTSDTLDESLTWADVVVIGPGLGQQEWGKTALEKVRGCDKPMLWDADALNLLAIAPDKRHNRILTPHPGEAARLLNRRVAEIESDRLLSARELAQRYGGCVVLKGAGTVVAGGHDECSIIDAGNPGMGSGGMGDVLSGIIGALLAQRLDPYDAACAGCVAHGAAADALAARYGTRGMLATDLFSTLYRFVNPDIDRPES",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should contain one or more YjeF N-terminal domains that are essential for its function.2. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.3. The designed protein should be optimized for NADPHX epimerase activity, NADHX epimerase activity, metal ion binding, ADP-dependent NAD(P)H-hydrate dehydratase activity activity in order to improve nicotinamide nucleotide metabolic process.4. The designed protein should have a binding site that is specific to the target (6S)-NADPHX, K(+), AMP ligand.5. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..6. The protein should have a low Km value for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "MVAIERQADASGLTYDLMMENAGRNLADAIAEEYPDSRDAGVLGLVGSGNNGGDTLVALARLAQQGWRASACIVRQRSPDDPLVLRLTQAGGNVFMLSPENGFALLEEQIQKHAILLDGVLGTGARLPLKPELAQVLGFVQTRVLENKDIRVVAVDCPSGVDCDSGEHAPESIPAHLTVTMAAVKRGLLQFPAYNLVGKLKVVGIGLGEEIPPPQGLGEINWGDRQWVASTLPRRPLDAHKGTFGTALIVAGSVNYTGAAWLAGRAAYKSGAGLVTMAVPQSLHTTLAGQFPEATWLLLPDELGVIAGDAWRVLLAGMERATAILIGPGFGLEETTRAFLANLFAAGAHTRRAGIGLVPSVKTAETQPSTKLPPVVLDADGLKLTARLPNWHKQVPAPAVLTPHPGEMSVLTGLTKETIQRERVKIAEQYARDWGHVVVLKGAFTVIAAPDGQTAVIAVATPALARAGTGDVLAGLIVGLRAQGLEAFPAAVAGAWIHAQAGLAAQEHLGSSASVLAGDVLSGVDAVMEPPDVGARWPVPAATDDKYTQGVVGVLAGSAQYPGAAILCSGAAVAATSGMVRYVGSAAADVVTHYPEVVAATGMKEAGRVQSWVVGPGFGTDTSSRDVLSQVLTSDLPVVVDADALTILSSSPELVRGRTAPTLLTPHAGEFERLTGAAPGSDRVSAVRELASTWGVSVLLKGRATVIAGPDGAVVVSDAGGSAASTAGSGDVLAGILGALLASGLDTVTAAACAARVHALAAATAAHGRGGEFAAPISASPLLGAVSDAIRT",
    "response#2": "MVIALCGKGNNAGDAYLVAMLAKLKGLEAQCIHFVAPNTLSGDAKSAYEQCHETEVHIAAYNCDTFRILLDKQTSDCIIVDGLFGTGLNRDLDANYADAIELSNTVRREKSNIHTISLDIPSGLSANTGRIFNACIEADLTTTFIANKIGLMTGVGRHYSGQIAFHDLNLIEGIFDKFEVLPMYIPRDKFSHKGTYGHSLMIGGSYGKIGAALLSSEACLKSGGGLVTSYIPECGYEIVQTALPEIMVITDEEEKFIGDISFDIEPTVIGIGPGIGTHEKTADALEKFLKENKTPLVVDADALNILSKNKELLKLLPGRTVLTPHPGELERLIGKWKDDFDKLKKAKAFSKKYKLILLIKGAHSIVVDADKLYINNTGNPGMATAGSGDVLTGIITGLISQGYEPLVATIFGTYLHGKAGDLAVIKTGYQALTATEIGDFIGDAYLDLFSHKGTYGHSLMIGGSYGKIGAALLSSEACLKSGGGLVTSYIPECGYEIVQTALPEIMVITDEEEKFIGDISFDIEPTVIGIGPGIGTHEKTADALEKFLKENKTPLVVDADALNILSKNKELLKLLPGRTVLTPHPGELERLIGKWKDDFDKLKKAKAFSKKYKLILLIKGAHSIVVDADKLYINNTGNPGMATAGSGDVLTGIITGLISQGYEPLVATIFGTYLHGKAGDLAVIKTGYQALTATEIGDFLLIGGGWGMAGALGLNIIGALSCGSTYLNVSTDESCYPCLASRFLTCVYHPFNEYNWEGIIKSILKNNNSIAFGSGSVRLPAKNKILNLLLESVKTTLVLDAEAINLLSRNYYVLNLTKAKVVLTPHIKEFSRLIDTPIEVIMQNKFEIAKDFCEKHNVILVLKGVHTIVVSNKYIYINQTGNQILARAGSGDLLTGMITALTSIINDTYLATIMAVWLHGYIADIAKERYA",
    "response#3": "LMIGGSYGKIGAALLSSEACLKSGGGLVTSYIPECGYEIVQTALPEIMVITDEEEKFIGDISFDIEPTVIGIGPGIGTHEKTADALEKFLKENKTPLVVDADALNILSKNKELLKLLPGRTVLTPHPGELERLIGKWKDDFDKLKKAKAFSKKYKLILLIKGAHSIVVDADKLYINNTGNPGMATAGSGDVLTGIITGLISQGYEPLVATIFGTYLHGKAGDLAVIKTGYQALTATEIGDVGVLAGSAQYPGAAILCSGAAVAATSGMVRYVGSAAADVVTHYPEVVAATGMKEAGRVQSWVVGPGFGTDTSSRDVLSQVLTSDLPVVVDADALTILSSSPELVRGRTAPTLLTPHAGEFERLTGAAPGSDRVSAVRELASTWGVSVLLKGRATVIAGPDGAVVVSDAGGSAASTAGSGDVLAGILGALLASGLDTVTAAACAARVHALAAATAAHGVAVIGGAPGMTGAPILAARTALKCGAGRVLVGFADNPPAYDSMQPELMCRHADDLDFSAAVLVLGPGMGMADSAQALLTRALDALSWLVLDADALNLLARQPQLQIQAAQRQHAILMTPHPLEAARLLGLSTSEIQADRVTAARKLACRFNAVVVLKGVGSIIARADGAVAVNPTGNPALATAGSGDVLSGICGALLAQGWPQWEAALAATWLHGRAADQLVEQL"
  },
  {
    "reference": "MEVIVPFSTERPKSRLSAVLTPDERAAFARTMLQDVLAAIDAADGEPRILATGAVGIDLPSPVEIDDRPLTDAVNAAIDARLGNDGAERVAVVMADLALATPNALRELFAAGREAEVAIAPGRGGGTNAFVAGHPAFRVDYHGASYLDHRRIADEIGAGVRVVDSHRLATDVDEPADLAELLIHAEADGDGGRAARWLRDAGFALDTTDGRVGVRRE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: It is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor.; Guanylyltransferase that catalyzes the activation of (2S)-2- phospholactate (2-PL) as (2S)-lactyl-2-diphospho-5'-guanosine, via the condensation of 2-PL with GTP.2. The designed protein must be able to perform GTP binding under specific conditions. The designed protein sequence is ",
    "response#1": "MRDVLLATHNPGKVREIRE",
    "response#2": "MDNQNQTPR",
    "response#3": "MXKXN"
  },
  {
    "reference": "MDLPRPKALLSHHGYDVGQGAIAGGAVAVVVVVCVGFLLVLLVIGVLKMRDTPMPRRRRQKRQSDGGMHWDDSGMNITVNPLDDVEKNGGAIDEFSDEEEEEETDGESECSYRDEEDDVSEDEEDQTEVLPHLDANQRVVGGLEWDDEDAISTNARSYRV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that can perform kinesin binding, X11-like protein binding in extracellular space, neuronal cell body membrane, intracellular membrane-bounded organelle, postsynaptic membrane to support protein localization, positive regulation of synapse assembly, positive regulation of synaptic transmission, associative learning.2. Prioritize Acidic residues compositional bias within the protein sequence to achieve desired characteristics.3. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "EDFFWQAGDMADRIDDLQEDLNRNDFAEDVPLARHAIDHHNEMRKKITKLPIEDLDMQGKKLLAKINAMAPPGGQAPTNSGGPDMDSGPSSAGQQSQPSQQSRSVGGNPDMSAAVNKALRQIELIHTGQEKLLMLWQHKKVKLC",
    "response#2": "EKDIFVVRENGTTCLMAEFAAKFIVPYDVWASNYVDLITEQAEISLTRGAEVKGRCGHNESELQVFWVDRAYTLKMLFVKESHNTSKGLEATWKLSKVQFVYDSSEKTHFKDAVSAGKHTANSHHLSALVTPAGMSYECQAQQTISLASSDPQKTVTMILSAVHIQPFDR",
    "response#3": "EKDIFVVRENGTTCLMAEFAAKFIVPYDVWASNYVDLITEQAEISLTRGAEVKGRCGHNESELQVFWVDRAYTLKMLFVKESHNTSKGLEATWKLSKVQFVYDSSEKTHFKDAVSAGKHTANSHHLSALVTPAGMSYECQAQQTISLASSDPQKTVTMILSAVHIQPFDR"
  },
  {
    "reference": "MKKYQFEVCANSVESCLAAQAGGADRVELCAGIPEGGTTPSYGEISTARDMLTTTRLHVIIRPRGGDFLYSPIEVRTMLKDIEMARQLGADGVVFGCLTANGEIDLPVMQELMKASQGLSVTFHRAFDICRDPEKALEQIIELGCNRILTSGQQATAELGIPLLKALQTQASGRIILLAGCGVNEKNIARIASETGIQEFHFSARESIKSDMKYKNESVSMGGTVHIDEYERNVTTAQRVINTIQAIKS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein can be expressed in cytoplasm and perform copper ion binding without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "FPTTPEKAYDFVNGAPNDIANDTQAANGTRAIVFEYGSRIQIIFQNTGTLTTENHPIHLHGHSFYVIGYGTGNYDQQTAKFNLEDPPYLNTIGVPVGGWAAIRFVANNPGLWLLHCHFDIHQTWGMSTMFIVKNP",
    "response#2": "SCETTVTSGDTMTYSTRSISVPASCAEFTVNFEHKGHMPKTGMGHNWVLAKSADVGDVAKEGAHAGADNNFVTPGDKRVIAFTPIIGGGEKTSVKFKVSALSKDEAYTYFCSYPGHFSMMRGTLKLC",
    "response#3": "YFTLDFPTTPEKAYDFVNGAPNDIANDTQAANGTRAIVFEYGSRIQIIFQNTGTLTTENHPIHLHGHSFYVIGYGTGNYDQQTAKFNLEDPPYLNTIGVPVGGWAAIRFVANNPGLWLLHCHFDIHQTWGMSTMFIVKNC"
  },
  {
    "reference": "MSMRPPDPGQASSRARARMVERLRAQGIADEKVLAAMMQIPRHAFVDEGLAFSAYDDTALPIGYQQTISQPLVVARMIEILRAGRELGRTLEVGAGCGYQAAVLSLVATEVFAVERIRPLLDRARENLRPLRLPNVRLKYADGNLGLPEAAPFDTIIVAAAAAGIPGSLKEQLAPGGRLMIPLGSADQRLVMTERHGNIFRESRFEAVRFVPLLTGTE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein is the optimization of the active site to achieve high catalytic efficiency for [protein]-L-isoaspartate + S-adenosyl-L-methionine =   [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-   homocysteine.2. For general function, the protein need meet that Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues; It plays a role in the repair and/or degradation of damaged proteins.3. The designed protein should have protein-L-isoaspartate (D-aspartate) O-methyltransferase activity within cytoplasm to regulate protein repair. The designed protein sequence is ",
    "response#1": "MRKFTGRINISGKGLHTGKECTMTFKPAPENSGYKFTRVDLEGKPEIPALIDNVVDTQRGTTLVKGDVRVSTVEHVLAAIVGLQIDNALVEVDGPEVPILDGSSKFFVEAIEKVGIKEQEAEREYFVLKEKISYKDEKTGSEIIAFPADDFEVNVMIDFNSPALGSQHATLKNLSNFKDEIAPSRTFSFLHEIEELANQGIIKGGDLNNAIVYVDKELSQDTLDKLKKAFNKDTISVKPKGILNNIELHYPNEPARHKLLDVVGDLALVGKRIRGRIIANRPGHSINTNFAKRISKLIKKEKRKKIPNFDITKEPVMDINKIKDLLPHRYPFLMVDKILELTDTSVVGVKNVTINEPFFTGHFPGNPIMPGVLQIEAMAQTGGVLVLNSVPDPENYLTYFMKIDKVKFKQKVVPGDTLIFKLDLITPIRRGICHMQGYAYANGKLVAEAELMAQIVKNK",
    "response#2": "MNSFSTFLESVRQDARLDIPEKASSEIASSIRTAINAGKSVDEITALGNQILSKHSATSAYREGYEGVPTAAQEFAITTGSIHPNAAKVAREAVSHVNPEDVQAHIDQGVYHTDGYFGTEVDRPITFENVSGVHIKHPEIKSLIDEGTRSGSMEIVNQLMKTDFEGGRQTLGYSDGRYVGIPQETAPDLVAEVQKQVESGEISREEADAILHQAYGQTVKSASVTGSPIAGQAVPKETAPDLVAEVQKQVESGELSREDAEAVIRQAYGKTVKSASVTGSPVAGQAVPKETAPDLVAEVQKQVESGEISKEEADAILHQAYGQKVEEASITGSPIAGQAVPKETAPDLVAEVQKQVESGQISREDAEAVIRQAYGQKVEEASITGSPIAGQAVPKETAPDLVAEVQKQVESGQISREEAEEILKQAYGQKVEEASITGSPIAGQVVPKETAPDLVAEVQKQVESGQISREEAEEILKQAYGQTVKEVSVTGSPIAGQAVPKETAPDLVAEVQKQVESGQISKEEADAILHQAYGQTVKSVSVTGSPIAGQAVPKETAPDLVAEVQKQVESGQISKEEAEEILRQAYGQTVKEVSVTGSPIAGQAVPKETAPDLVAEVQKQVESGQISKEEAEEILRQAYGQTVKTVSVTASPAAGQAVPKETAPDLVAEVQKQVESGQISKEEAEEILRQAYGQTVKEVSVTG",
    "response#3": "MDYIYPVLDKLINPDFKKEIVILLDKYKKENGKKLKLSDDIIKNIFQAATLLSSELGKNKPYINEELINGISQDLENNGYITHLIELTNLIPNITSLNLSNIRNKSKEIIDIIDNLIAICGFNVNSYLKIDNIEKIKSLESELTLLNNKLENLEEEREKLNQEIKEIEKSINEIKKFNNELNELNNLKNKAININEEFIKIKNIENEINKLEKNKKILEHEIKQIEKDIKEKNNEINILNQNIIKIKEKEKKLNEILEKFKKLKEKIEKERREYEKIIKNLNSLNKEKENIKNEINKIKNQISKLKEKINEINYNISSYNELKNKLEKLEREKEKLENEKKDLQKNVESLKKEKDEKEKIIKEKEKKLKELEQKIKEIEKMIEEFKKKLETMNEKMAELDKKLKEYENLKKEYAKKEEEIKNLENEKKNLQKEIDKLKNELNELKNKLNEIQKEINELKKKIEELNNKINELNNKIKEFENLKTTLEKQLKEAEEEFNKLKNEYNEKNKQLENYKKQLEELKKKIEELKKKIEELKNKIKELQNEINKLKGELNELKKKLNELKTKIEELEKKVKEVEEKIRKLEAELKRLKEELETLKKKLEELEKRLKELERKLEELEKKLEELKKKLEELKKKLEELKKKLEELKKKLEELKKKLEELEKKLEELEKKKKELENEKKELERKLNELKKKLEELEKKVEELRNKLKEYENLKK"
  },
  {
    "reference": "MTNPQGPQGPQQGNGGGRKPSQGGNPFGHNPFTGGGNDPQTGEKKPFWQSPWLWVVVLVLLVLSAFQMFSGTGSQTIDTKDGLTLLNGNTVSYAEIIDNRQLVKLTLSQNYTKTDSSTGKSRDYGKKVQFYYTFAQGSQIVKAVQSADPSDGWTSSMQQTSIWSYLITSILPFIIFFALFWFLLSRMGGANGMLGMGGKKNGKLLEGKTPTTKFSDVAGEQSAVEEVEEIKDFLKDPSKYKALGARIPRGVLLYGPPGTGKTLLARAIAGEAGVPFYAMAGSDFVEMFVGLGASRVRELFEEAKKNAPAIIFIDEIDAVGRKRGSGMGGGHDEREQTLNQLLVEMDGFDNDTNLIIIAATNRPDVLDPALLRPGRFDRQVGVEAPDLEGREAILKVHAKGKPFVPDVDLHMVAVRTPGFTGADLANVLNEAALLCARSGAQLIDNRAIDEAIDRVQAGPRRQSKGMALNELRNTAYHEGGHALVAAALRNTDPVTKVTILPRGRALGYTAVMPTTDRYSQSRNELLDQMAYAMGGRTAEEVVFHDPTTGASNDIEKATAIARKMVVEFGFSERLGAIQWASDDDQTTVMDGLQPRKYSDETAQVIDDEVLRLVENAHTEAWTIISQNRDVLDELVRQLLLKETLNEKELATIFSTIRKAPERALWLSSKDRPVSDLPPVEIPESLKRSVGMKAGE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of Zn(2+) is necessary for the protein to function in the desired environment.2. The protein should be able to bind Zn(2+) ligand in a variety of conditions3. The designed protein should have ATP hydrolysis activity, metalloendopeptidase activity, zinc ion binding, ATP-dependent peptidase activity within plasma membrane to regulate proteolysis, cell division.4. The protein should have a Helical domain that is long enough to span the entire membrane.5. The designed protein have AAA+ ATPase domains with specific sequence motifs that are crucial for its activity.6. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.7. The protein must exhibit the following characteristics: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins; Plays a role in the quality control of integral membrane proteins.. The designed protein sequence is ",
    "response#1": "MLMVGPPGTGKTLLAKAVAGEADVPFFSISGSDFVEMFVGTGASRVRDMFKKAKQAAPCIVFIDEIDAVGRQRGAGMGGGNDEREQTLNQLLVEMDGMEENIGIVIIAATNRPDVLDPALLRSGRFDRQITVHVPFLKESQRIELAKKDVTLENLAQRTPGFSGADLENVLNEGAILAVRGNRKLITMDDLDKAVLGVRNPEKLSEKEKENTAYHEAGHAILLELLEHTEPLHKVTIIPRGPSLGSTMWLPEEDKYTTRKNELLDQLVVTMGGRVAEEIVFGDVTSGARGDIGQATAIARKMVCEWGMSEKMGMVEYGEHDDYVFLGRDLGRSRGYSEATAQEIDREVRKLCDDAYARAKELLIKHRDRLEAIAKALLEYETLDGIHIKELTALRG",
    "response#2": "MLMVGPPGTGKTLLAKAVAGEADVPFFSISGSDFVEMFVGTGASRVRDMFKKAKQAAPCIVFIDEIDAVGRQRGAGMGGGNDEREQTLNQLLVEMDGMEENIGIVIIAATNRPDVLDPALLRSGRFDRQITVHLPYLEDTPQGGLLDFVAREGMQFYYDTSVGSAFLPDRIRPLLALAAKNMPRRYLEHFAEQALNAVPLREDGRLEIKAPRYAAFTRQ",
    "response#3": "MVMSEKEKLNTAYHESGHAIVGRLMPEHDPVYKVSIIPRGRALGVTMFLPEEDKYSHSKRYLDSSICSLFGGRIAEELTLGFEGVTTGASNDIERATSLARNMVTRWGLSEKLGPLQYDTDSDEPFLGRSAGQAQTVYSPETAQRIDEEVRNIIDTCYEKAKQILVDNRDKLDLMADALMKYETIDRFQIDDLIGQMKAV"
  },
  {
    "reference": "MTERAVALIVLAAGQGSRMLSDLPKVLHRLGGVPLVGHALAAGRSLDPERVIVVAGHGAEAVTKAVGRLNPEARIAIQPEQLGTGHAVAQALPLLEGFDGKVVVLYGDTPFIGEETLAALVSHPADVVVLGFEAADPGRYGRLVTKGDSLDRIVEFKEADEATRAIRLCNSGVMAVEADLLRDLVGRLGNDNAAGEYYLTDVVGLARAEGRDAAVVTCDEAETLGINTRAELAAAEAAFQSRLRAEALENGVTMADPATVWFAPDTVIGRDAVIGQNVVFGPGVTVESGAEILPFCHLEGCHVSAGATVGPFARLRPGAELGGDVHVGNFVEVKNSVLAEGVKVGHLTYLGDAEIGERTNIGAGTVTCNYDGVGKHRTVIGADAFIGSDTMLVAPVRVGARAMTGSGSVITADVPDDALALGRARQVTKPGLAARIMQALRAAKGKA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that can perform magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity in cytoplasm to support UDP-N-acetylglucosamine biosynthetic process, cell wall organization, regulation of cell shape.2. The protein with the reaction acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate, the Proton acceptor must be optimized to ensure efficient catalysis.3. We require a protein that can specifically bind to the metabolites produced in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway.4. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.5. The UDP-N-acetyl-alpha-D-glucosamine, Mg(2+), acetyl-CoA binding site should be located in a region of the protein that is accessible to the ligand.6. The designed protein should contain one or more MobA-like NTP transferase domains that are essential for its function. The designed protein sequence is ",
    "response#1": "VIVLAAGAGTRMKSKTPKILHEIGGRSLVGHALAGAAGIDPDHLIAVVSHERERVTAAIGEIATALGHEVLIAEQDEPLGTGDAARAGLTALPGDFDGTVVITVADAPLLDAPTLQALLDVHSGAAVLTLEDPDPTGYGRIIRTQDNEVMAIVEQKDATPEQRAVDEINAGIYAFRTAALRSGLSQLSTDNAQGELYLTDVIGLLRGDGKRVGAHVAADHREILGVNDRAQLAEARRQLNRRTLEAWMRAGVTVMDPATTWIEVDVQLAPDVTILPGTQLLGATVVEEDAVIGPDCTLQDVEVGAGARVVRTHGQLAVIGAGATVGPFSYLRPGTRLGAGGKIGAFVETKNAQIGDGAKVPHLSYVGDAEIGEGTNIGAGTIVANYDGVHKHRTVIGKHARTASNNTFVAPVTVGDGAATGAGSVIREDVPAGALAVTAGPGVALRDPSRLDVRGSLTCGHDVEIDVGCVFEGDVELGEGVRVGPHCVIRDSHIGAETVIEPHSVIEGAVVAGHNRIGPFARLRPGTRLAVGAKVGNFVETKNAEVGEGSKINHLSYVGDARLGRDVNVGAGTITCNYDGANKHRTEIGDEAFIGSNTALVAPVSVGRGATVGAGSTIDRDVADNALAVERSVPARVIPGTEE",
    "response#2": "IVLAAGEGTRMKSSLPKVLHKIAGLPLICHVIKQIELAGASQLAVVVGLGAKDVTETIQSFAKNAVIFEQKERLGTAHAALSARLALQEDIDDVLIIFGDTPLIERDSLLQIRAQLTSGADVVVAGFHAPNPTGYGRLLQNGQGFVKRIVEEKDATDEEKAVTEINSGVYVFKAAALKEALGKLNNNNAQGEYYLTDAFEILRSEGHELADAEAVVQDRLRRAAMDAGVTLTAPETVFLSGDTVIEADVTIEPHVVFGAGVIVRSGAVIRSFSHLEGCEVGPEGRIGPYARLRPGTRLGRDVHIGNFVELKATQMEDGAKAGHLTYLGDASIGARTNIGAGTITCNYDGFFKHRTTIGSDAFIGSDSIIVAPVRIGDGALTAASSVITNDVPAGDLAIARAVQKIIEGWVYDKRKRSKE",
    "response#3": "MKSIILAAGQGTRMKSSTSKVLHEIFHKPLVYYPIEAARYAGAQEVCLVVEHKSEEVMKTFGDTVKYALQAERLGTGHAVMQAMDFIGDDGEILVLYGDTPLITAQTIEKMLAFHRKKKNAVTVLSALVDDPTGYGRIVRDESKHFVKIVEQKDATDEEKRITEINGGMYVFEAKHLKSALSKITNNNKQQEYYLTDTIEILLHEGYNVQAYKTEDFIEIAGINDRAQLSEAERLMRKRINEKHMLNGVSLIDPARIDIRGELLCGSDVEIDINCIFEGTVKLGNFVRIGANCILKNVTVASGSIINPFSMVEDAEIGENCKIGPYARIRPGTRLASKVQIGNFVEVKNSQIGTGSKANHLSYIGDSGIGKNVNIGAGTITCNYDGVNKHHTIIEDDVFIGSDTQLIAPVKISKGTTIGAGSTITKETPENQLTLSRSKGVPARVIKR"
  },
  {
    "reference": "MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLNIVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTFGQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLKAGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQHGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFGNPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYTGTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDYGVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVSTSYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLDQFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVITLVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDEVMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINVLKTI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein design must be focused on enhancing the efficiency of protein secretion.2. The protein must exhibit the following characteristics: Necessary for the secretion of IPA invasins.. The designed protein sequence is ",
    "response#1": "LVIRLGTNAQQPVEWLVWSAKEEEIIASGILASAHALGELRERAGGRPVVTLVPGSDLIFRRVSLPGKYSRQAAAALPYLLEEQIASDVDELHLVVLGHEGHEVDLMAVDKEKMQIWLGWLQQAGLKSQQLLPDVLALPPAADGWSALQLGKEWLLRQGPCQGIVADESLLAMLLAAEADPVTIHSHTPLPAIPAANWLAADPELPMLLLAKGALNCQANLLQGPYRPL",
    "response#2": "LVIRLGTNAQQPVEWLVWSAKEEEIIASGILASAHALGELRERAGGRPVVTLVPGSDLIFRRVSLPGKYSRQAAAALPYLLEEQIASDVDELHLVVLGHEGHEVDLMAVDKEKMQIWLGWLQQAGLKSQQLLPDVLALPPAADGWSALQLGKEWLLRQGPCQGIVADESLLAMLLAAEADPVTIHSHTPLPAIPAANWLAADPELPMLLLAKGALNCQANLLQGPYRPL",
    "response#3": "TLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQKLGLVFNVIVSVL"
  },
  {
    "reference": "MSNQKLFNVAVVGATGAVGEQIIGLLEKRDFPIAELKLLSSARSAGSKIIFKGKEITVEEASPDSFKGIEIALFSAGGDVTKALAPHAVEHGAVCIDNTNAYRMDPDTPLVVPEVNIDKVNDHKGIIANPNCSTIQLVAALKPLQDRYGISRIIVSTYQAVSGAGSRAIDEMLRQTREVLDGNELIPDILPVGSLPVKHQIAFNAIPQIDKFQDNGYTLEEMKMVRETKKILDDESIDVTATCVRIPVVYGHSESVYVELKQNYELEDVRTLIADAPGVELVDDPAGQRYPLATEAAGKPEVFVGRLRRDLGHALGLNLWIVSDNLLKGAAWNAVQIAEYIAIEKE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The NADP(+), phosphate binding site should be stable and able to withstand changes in environmental conditions.2. The designed protein should contain one or more Semialdehyde dehydrogenase NAD-binding domains that are essential for its function.3. The designed protein must possess aspartate-semialdehyde dehydrogenase activity, NADP binding to support threonine biosynthetic process under certain conditions.4. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.. The designed protein sequence is ",
    "response#1": "KTAVVGATGMVGRTMMKLLEERNFPVTEIIPAASEKSVGKELMFKGKPVKVVSVMDAVKAKPVFAIFSAGASTSKEWAPVFAKNGIIVIDNSSAWRMDKDVPLVVPEINSHVIKKEDKIVDGFCVRIGAMRCHLAPLHRKYKIKRIVAATYQSVTGTGVKAVTQMENERKGIKGEMAYAHPIDMNCFPHGGSFQPDTYTTEEQKLIDETRKILEDQSIMVTATVVRIPVVGGHSEAVNVEFEKEYDLQEAISLMSKFPGVVIYDNPAENKYPMPILSHNRDEVFVGRIRRDHSREKCLNLWIVSDNIRGAAEGLLQENLVEWISPMTYQAASGAGARNMRELISQMGSIRDSVGGLLDDPASAILEIDRTVSSHMNSDGYPTDNFGVPLAGSLIPWIDTQLPSGQSREEWKAEAETNKILGRSDNPVPIDGLCVRIGAMRSHSQALTIKMKRDIPVSEIESIMAQANDWVKVIPNDRDLSMVELTPAKVTGTLSVPVGRVRKLTMGPEYISAFTVGDQLLG",
    "response#2": "MANLKPLHEAATIERINVATYQAVSGSGKEAIDELATQTANLLNGKPVECEAYPKQIAFNCLPHIDVFLDNGYTKEEMKMVWETQKIFGDDSILVNPTAVRVPVFFGHSEAVHIETREKLTEAKARELLSQFEGIEVLDDREPGGYPTAVTEAATHDPVYVGRIREDISHPRGLNLWVVADNVRR",
    "response#3": "LGGLFKAGLVEWLSSMTYQAASGAGAPNMRELLSQMGVLSGVVAEELKNPSSAILEIDRKVTETLRNGSLPVKEFGFPLAGNVLPWIDREVEDGQSREEWKGFAETNKILGATSPIPVDGICVRVGAMRCHSQALTIKLTKDLPLAEIESLIANDNEWVKLVPNNKADTLAQLTPAAISGTLTVPIGRVRKMKMGPQYLQAFTCGDQLLA"
  },
  {
    "reference": "MKCLVCCSYASSRNFGALSCSACKMFFTRATKNYAKFTCKYDKKCFESFTILPKCQFCRFKKCLEIGMRFSEPKQQLFNLNNSVDQDLIILLGNLAVKDGVHYSNFLNFYSLEDPSMDDILADRSRMKLMKRTLEIKTKSHEWTFLGSYYKIAQFLDFDFVKTMSLADRKILFSYNTLRMGSLSRSMRTYLDKRDCLMTPSGDDLFSPAVRGLFDKTPEIINRILCQVVGRLMEIKITYEEYLLLIMIVFCNPSISHELSDSARETLSKYQNMYASFLFRFCQLKNQHKAPTRFNDILSICNVNNKNVEDYCFVHMMFQCMVPEFKFKKLVNDIIIRS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must be optimized for sequence-specific DNA binding. The designed protein sequence is ",
    "response#1": "QRANQLVQEALEKRWIAELAEYQTVLPEQKYGSENSRIDFLLKADNQPDCFAEVKSTTLLTENDLGMFPDAKTERGQKHLRELAAIAESGQQAVILFAILHTGIQRFAVAKQIDPQYAALFEQAKNVGVKVLAYKAQII",
    "response#2": "QRANQLVQEALEKRWIAELAEYQTVLPEQKYGSENSRIDFLLKADNQPDCFAEVKSTTLLTENDLGMFPDAKTERGQKHLRELAAIAESGQQAVILFAILHTGIQRFAVAKQIDPQYAALFEQAKNVGVKVLAYKAQIL",
    "response#3": "ELAVSCARELAKMCDINEPLWNKKRLDNESVCLNEEEYKKMFLWPLMNDDDRFRREASRANAVIMLNCITLVKAFLDADKWSEMFFPIVSSAKTAQIISSGASGPSGTLLLMFAELQVVSPLVPTREAYFLRYVEQNAEEGKWMVVDFPIDRIKPASATTTDQYRRKPSGCIIQAMRNGYSQVTWVEHVEVEEKHVQDEVVREFVESGVAFGAERWLSVLKRQCEV"
  },
  {
    "reference": "MKVAIIGATGYGGIELIRLLEQHPYFSIASLHSFSQVGEFITNVYPHFRNVLVHTLQEIDAEKIEKEAEIVFLATPAGVSAELTPKLLAVGLKVIDLSGDFRMIDPSSYELWYKRPAAQEELLRKAVYGLSEWKRPEIQNANLIANPGCFATAALLAVAPLVRSGIIEEASIIIDAKSGVSGAGKTPTTMTHFPELYDNLHIYKVNQHQHVPEIEQMLTEWNRETKPITFSTHLIPISRGIMITLYAKVKEEMKIEQLQKLYEETYEYSAFVRVRSQGEFPSPKEVRGSNYCDIGIAYDERTERVTVVSVIDNMMKGAAGQAVQNANLLAGLEETTGLQHMPLYP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that can modulate the overall flux of metabolites through the N(2)-acetyl- L-ornithine from L-glutamate: step 3/4, L-arginine biosynthesis pathway, enhancing or suppressing its activity as needed.2. The designed protein is the optimization of the active site to achieve high catalytic efficiency for N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate =   H(+) + N-acetyl-L-glutamyl 5-phosphate + NADPH.3. The designed protein should have protein dimerization activity, N-acetyl-gamma-glutamyl-phosphate reductase activity, NADP+ binding within cytoplasm to regulate arginine biosynthetic process. The designed protein sequence is ",
    "response#1": "RVAVIGASGYGGLQTIRLLQDHPSLTVTFLGGERSAGRRWSSICSFLPLPEDPVVQSADAERIAAAADYAVLSLPNGLACQLAPELLQRGVRVVDLSADFRYRSLEQWSQVYAQEANRLSREDSELCQQAVYGLPEWHGPAIAEAMIAGPGCNVTATTLALQPLLAAGLVDPTSVVVDAKSGTTGAGRGLAEASHFCEVNENFRVYKPQHQHTPEIEQQLSELAGQSVRITFTPHLVPMTRGILSTIYARLRDPVTADQLRGLYEEFYAGEPFVRVLPEGVLPRTQHVRGSNYCHVGVEVDSRTGRAVVISVIDNLIKGAAGQAVQNMNLMFGLPETLGLPALPVYP",
    "response#2": "MGYKVGIVGGTGYTGVELLRLLAVHPKVEVTLITSRAEAGKCVTELFPTLRGHYDDLYFSEPESHALGACDLVFFATPHGVAQAMMKEVLAAGVRAIDLSADFRIRDIEVWEKWYGQQHGCPELVAQAVYGLPEIHREQIKDSIAGPGCNVTATTLALQPLLKQNIIETSSIIIDAKSGLSGAGRGLSETSHFVNINESITPYKITTHQHVPEIEQGLSQIQNKTISFTPHLLPMIRGMQSTIYAELKDQVTESDLKDLYQTFYKNEPFIRIKDSLPHTKYVKGTNYCHIGLSIDSRTGRLVVVSVIDNLMKGAAGQAVQNMNIMFGLPETAGLSFVPMFP",
    "response#3": "TLGIVGARGHTGAELIKLVAAHPRLELAFVSSRERAGQRLSDHHPEFQGELQYENLDADAVAAKGVDAVILALPNGLAAPFVAALEAAKPDTVIVDLSADYRFDNSWYYGLPELTRGRYNGRPRGIIANPNCTTLSMIVAIGRAAIVGGTGYTGVELLRLLANHPNVEIVAITSRTEAGRPVSKLFPNLRGYLDICFTEPEPAQLAAECDVVFFATPHGVAMDMVPALLAQNTRVIDLSADFRLADPTIWEQWYGRPHAAPHLLAEAVYGLPEINREAIRQGAALNTYEIAEELVAS"
  },
  {
    "reference": "MRKFTGDTLLVATHNTGKLEEIAALLEPYGVSVVGAAEKGLAEPEETETSFVGNARIKAHAAARATGLPALSDDSGLEIDALGGAPGVYTADWAETGEGRDFVMAMTRAHEELVASGAAQPWRARFCCTLVLAWPDGHDEVFPGTIDGQITWPMRGDQGHGYDPIFRPDGYEETFGEMDRWEKNKISHRADAFAKFVTGCFG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate binding.2. The protein should have a low Km value for dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MKP",
    "response#2": "LNSLF",
    "response#3": "EHRVLG"
  },
  {
    "reference": "MGNQDGKLKRSAGDASHEGGGAEDAAGPRDAEITKKASGSKKALGKHGKGGGGSGETSKKKSKSDSRASVFSNLRIRKNLTKGKGACDSREDVLDSQALPIGELDSAHSIVTKTPDLSLSAEETGLSDTECADPFEVIHPGASRPAEAGVGIQATAEDLETAAGAQDGQRTSSGSDTDIYSFHSATEQEDLLSDIQQAIRLQQQQQQKLLLQDSEEPAAPPTAISPQPGAFLGLDQFLLGPRSEAEKDTVQALPVRPDLPETTKSLVPEHPPSSGSHLTSETPGYATAPSAVTDSLSSPAFTFPEAGPGEGAAGVPVAGTGDTDEECEEDAFEDAPRGSPGEEWVPEVEEASQRLEKEPEEGMRESITSAVVSLPGSPAPSPRCFKPYPLITPCYIKTTTRQLSSPNHSPSQSPNQSPRIKKRPDPSVSRSSRTALASAAAPAKKHRLEGGLTGGLSRSADWTEELGVRTPGAGGSVHLLGRGATADDSGGGSPVLAAKAPGAPATADGFQNVFTGRTLLEKLFSQQENGPPEEAEKFCSRIIAMGLLLPFSDCFREPCNQNAGSSSAPFDQDQLYTWAAVSQPTHSMDYSEGQFPRREPSMWPSSKLPEEEPSPKDVDTEPKSSILESPKKCSNGVQQEVFDVKSEGQATVIQQLEQTIEDLRTKIAELEKQYPALDLEGPRGLSGLENGLTASADVSLDALVLHGKVAQPPRTLEAKSIQTSPTEEGRILTLPPPKAPPEGLLGSPAAASGESALLTSPSGPQTKF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should contain one or more FH2, FH1 domains that are essential for its function.2. The protein should exhibit Basic and acidic residues, Pro residues compositional bias.3. Design the protein with a signal peptide incorporated.4. A protein that contributes to cellular response to hypoxia, negative regulation of protein catabolic process, formin-nucleated actin cable assembly, intracellular transport by functioning in actin cytoskeleton, cytoplasmic vesicle membrane, cytosol, nucleus with actin binding, microtubule binding.5. The protein must exhibit the following characteristics: Protects cells against apoptosis by protecting CDKN1A against degradation .; Plays a role in responses to DNA damage, cellular stress and hypoxia by protecting CDKN1A against degradation, and thereby plays a role in stress-induced cell cycle arrest ; Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport; Actin-binding protein that is involved in actin cytoskeleton assembly and reorganization; Acts as an actin nucleation factor and promotes assembly of actin filaments together with SPIRE1 and SPIRE2; Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage . The designed protein sequence is ",
    "response#1": "MSVKEGAQRKWAALKEKLGPQDSDPTEANLESAEPELCIRLLQMPSVVNYSGLRKRLESSDGGWMVQFLEQSGLDLLLEALARLSGRGVARISDALLQLTCISCVRAVMNSQQGIEYILSNQGYVRQLSQALDTSNVMVKKQVFELLAALCDPTEANLESADPELCIRLLQMPSVVNYSGLRKRLEGSDGGWMVQFLEQSGLDLLLEALARLSGRGVARISDALLQLTCVSCVRAVMNSRQGIEYILSNQGYVRQLSQALDTSNVMVKKQVFELLAALCSDPTEANLESAEPELCIRLLQMPSVVNYSGLRKRLESSDGGWMVQFLEQSGLDLLLEALARLSGRGVARISDALLQLTCISCVRAVMNSQQGIEYILSNQGYVRQLSQALDTSNVMVKKQVFELLAALCSDPTEANLESAEPELCIRLLQMPSVVNYSGLRKRLESSDGGWMVQFLEQSGLDLLLEALARLSGRGVARISDALLQLTCISCVRAVMNSQQGIEYILSNQGYVRQLSQALDTSNVMVKKQVFELLAALCEGHALTLDALDHYKMVCSQQYRFSVIMSELSDSDNVPYVVTLLSVINAIILGPEDLRSRAQLRSEFIGLQLLDILTRLRDLEDADLLIQLEAFEEAKAEDEEELQRISDGINMNSHQEVFASLFHKVSCSPASAQLLSVLQGLMHLEPAGRSGQLLWEALENLVNRAVLLASDAQACTLEEVVERLLEAEGHVLTLDALDHYKTVCSQQYRFSIVMNELSGSDNVPYVVTLLSVINAVILGPEDLRARTQLRNEFIGLQLLDVLARLRDLEDADLLIQLEAFEEAKAEDEEELLRVSGGVDMSSHQEVFASLFHKVSCSPVSAQLLSVLQGLLHLEPTLRSSQLLWEALESLVNRAVLLASDAQECTLEEVVERLL",
    "response#2": "MSVKEGAQRKWAALKEKLGPQDSDPTEANLESADPELCIRLLQMPSVVNYSGLRKRLEGSDGGWMVQFLEQSGLDLLLEALARLSGRGVARISDALLQLTCVSCVRAVMNSRQGIEYILSNQGYVRQLSQALDTSNVMVKKQVFELLAALCIYSPEGHVLTLDALDHYKTVCSQQYRFSIVMNELSGSDNVPYVVTLLSVINAVILGPEDLRARTQLRNEFIGLQLLDVLARLRDLEDADLLIQLEAFEEAKAEDEEELLRVSGGVDMSSHQEVFASLFHKVSCSPVSAQLLSVLQGLLHLEPTLRSSQLLWEALESLVNRAVLLASDAPKKEFKPEISMRRLNWLKIGPNEMSENCFWIKVNENKYENKDLLCKLENTFCCLEKEKRDTNDFDEKKVIKKRMKELKFLDPKIAQNLSIFLSSFRVPYEKIRTMILEVDEAQLSESMIQNLMKHLPDEEQLKSLSQFRSDYNSLCEPEQFAS",
    "response#3": "MSVKEGAQRKWAALKEKLGPQDSDPTEANLESADPELCIRLLQMPSVVNYSGLRKRLEGSDGGWMVQFLEQSGLDLLLEALARLSGRGVARISDALLQLTCVSCVRAVMNSRQGIEYILSNQGYVRQLSQALDTSNVMVKKQVFELLAALCIYSPEGHVLTLDALDHYKTVCSQQYRFSIVMNELSGSDNVPYVVTLLSVINAVILGPEDLRARTQLRNEFIGLQLLDVLARLRDLEDADLLIQLEAFEEAKAEDEEELLRVSGGVDMSSHQEVFASLFHKVSCSPVSAQLLSVLQGLLHLEPTLRSSQLLWEALESLVNRAVLLASDASGESSNGQVKLKPLHWDKVNPDSDHSMVWDKIDRGSFSFDGDLMEALFGYVAVGKKSPDDGGDKKPSSASPAQIFILDPRKSQNTAIVLKSLGMTRDELVESLMEGHDFHPDTLERLSRIAPTKEEQSAILQFDGDTKMLADAESFLFHLLKAVPCAFTRLNALLFRANYYPEISNHNKNLQTLDLACTELRSRGLFVKLLEAILKSGNRMNAGTARGDAQAFNLTALLKLSDVKSVDGKTTLLNFVVEEVVRSEGKRCVLNRRTNRSFSRSSSSSISEVISKEEQEKEYLRLGLPVVGGLSSEFTNVKKAAAVDYDTVAATCLALTSRAKDARRVLAQSEGDNKEGVRFVKKMNEFLDSVEEEVKLAKEEEKKVLELVKRTTEYYQAGAVKGKNPLHLFVIVRDFLAMVDKVCVEIARNLQRRS"
  },
  {
    "reference": "MLPDHFSPLSGDIKLSVLALVVLVVLAQTAPDGWIRRCYYGTGRCRKSCKEIERKKEKCGEKHICCVPKEKDKLSHIHDQKETSELYI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Has antibacterial activity.. The designed protein sequence is ",
    "response#1": "PSRMTLLLGPPSSGKTTLLLALAGKLDKSLDVSGEVTYNGYRLNEFVPIKTSAYISQNDLHVGIMTVKETLDFSARCQGVGTRYDLLNELARREKDAGIFPEADVDLFMKASAAQGVKSSLITDYTLKILGLDICKDTIVGDDMMRGISGGQKKRVTTGEMIVGPTKTLFMDEISTGLDSSTTFQIVKCLQQIVHLTEATVLISLLQPAPETFDLFDDIILLSEGD",
    "response#2": "LQSKRDIGIDTTAVLLKHLMGSRSNYCPDGIFTILAQGPMLEPVMETALKVSSGLQTAKRTPIPALILSKGTQAVMRLFLLGLRPARYCLRAFMLKALEGLHLLADLVRGHNPVN",
    "response#3": "PSRMTLLLGPPSSGKTTLLLALAGKLDKSLDVSGEVTYNGYRLNEFVPIKTSAYISQNDLHVGIMTVKETLDFSARCQGVGTRYDLLNELARREKDAGIFPEADVDLFMKASAAQGVKSSLITDYTLKILGLDICKDTIVGDDMMRGISGGQKKRVTTGEMIVGPTKTLFMDEISTGLDSSTTFQIVKCLQQIVHLTEATVLISLLQPAPETFDLFDDIILLSEGQ"
  },
  {
    "reference": "MTRVLHDTDEALAWLRERTTGTLQSDSRQVAPGDAFIAWPGAATDARAHLADARTRGAAACLVEAEGGEAFDLAGDDIATFQGLKAAVGEIASAWFGVPSHGLEMVAFTGTNGKTSSAWWLAQALSGCELTAHAPCALVGTLGMGFPAALEETGLTTPDPVRLQRGLRALVDAGARSCAIEASSIGIAEHRLAGTRIAVAVFTNFTQDHLDYHGSMAAYLDAKRALFAWPGLRAAVLNLDDAAAASLQGQMEAAGLDLWTVSQTGPARLRASEVALGAQGLQCLLSEGDEQLRLHTTLIGQYNISNLLGVIAAMRALGVPLAAAVRACEQLAPVPGRMERLLAAGQPLVAVDYAHTPDALEKALQALRPLAAERGGQLWCVFGCGGDRDAGKRPLMGAAAQQHADQVVVTSDNPRSEDPAAIVHQILLGTIAGNTVRAEPDRAAAIAQVLQQAAPADVVLIAGKGHEAWQEMAGVKRPFSDLEHARTALARRGVAA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The desired protein should be able to perform ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate2. The ideal protein would be one that plays a critical role in peptidoglycan biosynthetic process, cell division, cell wall organization, cell cycle, regulation of cell shape through UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity within cytoplasm.3. The designed protein should have a binding site that is specific to the target UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate, ATP ligand.4. The designed protein have Mur ligase C-terminal, Mur ligase central domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MRLRDLVSGVTADSRAVTPGSLFCCVRGTKSDGHDFAPAAVAAGATAVICERPLDLAPHVVQAVVADGRAALAAAAAAFHRHPSRSLRVSGVTADSRAVTPGSLFCCVRGTKSDGHDFAPAAVAAGATAVICERPLDLAPHVVQAVVADGRAALAAAAAAFHRHPSRSLRITGTNGKTTTTQWLAQLLQLLGTPAASVGTLGFGRVGEEVVATGLTTPDAVSLQRILRQMLDQGTAAVVMEASSHSLDQRRLAGVSVDVAVFTNIGRDHLDYHGDMNSYVAAKLKLMDFPSLQSAVVNADDPVAAEFIARAAGHCRVLTCSLQGKADFMVSQVRYHAGGIDAELVTPEGEFDLQLAIWGEFNLSNLLVVIAALAEEGICGRFERIDAGQPFALVVDYAHTPDALEKVLTAARGMTGTTRSPGRVIVVFGCGGGRDRAKRPLVGEVAARLADLAILTSDNPRHEDPLAIIDEVLTGVGTAFEASLVVQPDRGAAIEEAVARAEPGDVVVVAGKLAAWRAECAA",
    "response#2": "MKLTELMSGLGYQEVKGDPSVALQLDSRQVQPGDSFLAVSGTLTHGEKFIADARDRGARVIFESGDNYHAMTDAQQVLRVRVPQLGERISELAGRYYGSPSRDMRLIGVTGTNGKTTVTHLIETIMQDAKKKVGLIGTVAYYIGDREEKASHTTPESLELQRLLARMVEEGCEYCVMEVSSHALDLHRVEGCHFAVGIFLNLTQDHLDFHKTFEEYMKSKRRLFTMCDNAIINIDDEYGKRLAEEAECAIETYGFSKGSDYRGSNLRLEGKHARFEVRTPWGESDLELGIPGEFSVYNSLAAIAVCHILGVEEEAILRGLREVRVPAGRMQRVERGQDFLAIVDYAHKPAALESVIATLRGHLAADTAGRLAVVVGAGGDRDAAKRPLMGAAGARGADLLIVTDDNPRTEDPAAIRAAVRSGALEVPAAERGEVREIGDRAEAIAAAVAWARPGDAVLIAGKGHEDYQILGTEKIHFDDREEARKALEDKGGRA",
    "response#3": "MKLRELIEGIDIKNVVGDINVDIEVTGVNHDSRSVAQGDLFACISGAEHDGHSYAIDAINGGAAALLVDRELNLAIPQIVLVTDDSRRVERGACFVAVPGLKHDARRFVPEAVERGAALVVTEGEAMAGLAVAQVLVPSARVALGRLASATYFGYPAEKLKVIGVTGTNGKTTVTHLIETIMQDAKKKVGVIGTLGARIGNEVLPAERTTPESLELQALLREMVDNGCDYVVMEVSSHALALHRVDDCVFNTMIFTNLTRDHLDFHKTMENYYQAKKILFDRLNRDAVAVFNRDDEYGQRLLLETKGRVFTYGIDNKADFRAENIKMSADGTRFDLVYKGETFPVDVRLIGKFNVYNALAAIGAALVLGVDMENILEGISRSEGVSGRVERFKATDDQPLIVVDYAHTPDALDQVLQALRPHVAGKLWCVFGCGGDRDRGKRPQMGAIAERLADRVILTDDNPRHEAGDAIVREIAGGMRSSARIIRDRREAIATALSEADAADVVLVAGKGHETYQILKDKTIPFDDRKVALEAIKSRRK"
  },
  {
    "reference": "MDLVTLMSGNANTELSGKIASYLGRELCDVEVDRFSDGEIRVNIHENIRGKDVFIIQPTLPPADNLLELLILIDACYRASANRITAVIPYYGYARQDRKDQPRVPITAKLVANLIETAGAHRVLALELHAAQIQGFFDIQVDNLFAAPVMLEYIREKNFNDLTVVSPDVGGIKMGRAFAKRLDANLAIVDKRRTGADESEVMNVIGEVAGSDIIILDDIISTAGTITQAAQVLKDAGAGSIMAMATHPVFSGQALEKLENSCIEEVVVTNSIPFKDNGECSRIKVLDVAPLLGEAIKRIHMEESISMLFV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be designed to catalyze the following reaction ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-   diphosphate + AMP + H(+).2. The designed protein must be optimized for ribose phosphate diphosphokinase activity. The designed protein sequence is ",
    "response#1": "ASPILLSDLREKNYSDLIVVSPDIGGVVRARALAKLMDCDLAIIDKRRPKPNVSEVMNVIGEIDGRNCIIMDDMVDTAGTLTKAAEVLKARGAKRVMAYCTHPVLSGPAIQRLQSSAIDELVVTNARLAPR",
    "response#2": "MLFSGTSNPELAEKMAECLDTEPGRVKIRRFKSGEIYIHYEESVRNADVFLVQTMSRPINDHLMELLLMVDAAKRASARSINAVIPYFGYARQEKKDAPREPISAKVVADLLHTVE",
    "response#3": "LLSGYFRDKALDDLVVVATDIGISKRARDMAAKLNAPLAIIEKRRVGNAERVDTLNVIGDVKGKCALTVDDEIDTAGSLTGAVEALLEQGAKEVYSCCTHAVLSGSAVERIAA"
  },
  {
    "reference": "MENSNQVLDEKTIAVIKQTVPVLQAHGNEITKTFYKRMLTGHPELNHIFNQTNQSKGAQPRALAQTVYAAAANIDQLDTILPHVKQIAQKHTSLNIRPEQYPIVGHYLLGAIKEVLGAAADDTILDAWAKAYGVIANIFIFIEKEMYTQKRVQVGGWEGFRDFTVIKKRKESDVITSFYLKPTDDGPVAAFEPGQYITVKAHIEGQSFTQLRQYSLSDAPDKAYYRISVKREDGSDQCPSGIVSTYLHRHVQEGDILPLSAPSGEFTLQHQETKPLVLISGGVGLTPLMSILETVIEKQPERQVLFIHAAHTGTQHAMKDRVRTIAKERSNVTAYTVYAQPEEKDIFDKQGRIDDAWLQQVLPASPASYYFCGPKGFMKDIYLALKKKNVPTTDIHFEIFGPAEDITA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein with nitric oxide dioxygenase activity, FAD binding, heme binding, oxygen carrier activity, metal ion binding is needed to enhance response to nitrosative stress, response to toxic substance in a specific context.2. The protein requires heme b to activate a critical catalytic site.3. For general function, the protein need meet that Therefore, plays a central role in the inducible response to nitrosative stress.; Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds4. The designed protein have FAD-binding FR-type, Globin family profile domains with specific sequence motifs that are crucial for its activity.5. We need a protein that can perform NADPH + 2 nitric oxide + 2 O2 = H(+) + NADP(+) + 2 nitrate with high efficiency.6. The NADP(+), FAD binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MLDSQTIAIVKSTIPVLVETGPKLTGYFYERMFKHNPELKDIFNLSHQQSGAQREALFGAICAYASNIENLSAILPAVERIAQKHASFNIQPEQYNIVGHHLLATIDELLHPGTEVIEAWGKAYQVLANVFIQRLEDERAQQAEFKVVAKVEESEEITSFVMAPADGGKVVAHQPGQYIGMLANVNGGEQRRQYSLSAAANGETYRISVKREPGGLVSNFLHDQVKVGDSIQVFPPAGDLISAGVGQTPMMAMLQHLLTSKSLRKVGYIHAARNGKVHAFKQELRKLANAHDNLKTVLFYEQPDTQDIIGLDYDYTGRIDEDAILLRIYQENADYYLCGPVGFMQQQVYGDLKKLGIRDDQIHYEFFGPHQEV",
    "response#2": "MLSQKTIEIIKSTVPVLEVHGTAITKRFYQMMFAKHPELLNIFNHANQKQGRQQAALANAVYAAALYIDKLETILPVVKQIGHKHRSLGVKAEHYPIVGENLLAAIKDVLGDAATDEILQAWAEAYGVIADAFIGVEQEMYEETKSFEGGWDFFKDFVVADKVVESDVITSFYLKPADGKKLPSYKPGQYITVRVKVPGEEYLLNRQYSLSQAPREDVFRISVKREDECAPHGKVSVFLHRNLEIGDQLEVSAPAGDFFLEQEDDRPVVYLISGGVGITPMMSMLKRVLERDPTREIIFLHGARNSAVHAMRERLEELAASHENLSVHVCYDEPTDQDIPGRDYDKEGYIDLDLLRTIVPEDDADFYFCGPIPFMRMQHDALKKLGVEDERIHYEFFGPAGSLE",
    "response#3": "MLDAKTIEIIKSTVPVLEVHGQAITTRFYELLFTSHPELLHIFNHANQKQGRQQAALANAVYAAAAHIDQLETIVPVVKQIAHKHRSLGVKAEHYPIVGENLLQAIKDVLGDAATDEIIGAWGTAYGVIADVFISPEDQRMYEEALKEKGLREFRIVKKQKESDVITSFELAPVDNKPVVDYAAGQYIAVYLQGEQLENQEIRQYSLTRSPNGKTYRIAVKREDQGKASNYLHNVIQEGDILSLAPPYGDFFIDQKDRVLISGGVGLTPMVSMLETLAEKYPDRSVTYIHAAINGNIHAMKKQVQELAESHENVTTHVCYEKPLVSDSEGRFDKEGYIDYELLKDIVPSKDADIYVCGPTPFMKTINKALNEWKVPKERIHYEFFGPADSL"
  },
  {
    "reference": "MSTVGGIEQLIPDSVSTSFIETVNGSHQFTIQGYSLAKGMSPGKFIQSDIFSVGGYDWAIYFYPDGKNPEDQSSYISLFIALASDSNDIRALFELTLMDQSGKGKHKVHSHFDRALEGGPYTLKYKGSMWGYKRFFKRSALETSDYLKDDCLVINCTVGVVRARLEGPKQYGIVLPLSNMGQGLKDLLDSEVGCDIAFQVGDETYKAHKLILAARSPVFRAQFFGPIGNNNVDRIVIDDIEPSIFKAMLSFIYTDVLPNVHEITGSTSASSFTNMIQHLLAAADLYDLARLKILCEVLLCEKLDVDNVATTLALAEQHQFLQLKAFCLEFVASPANLGAVMKSEGFKHLKQSCPTLLSELLNTVAAADKSSTSGQSNKKRSASSVLGCDTTNVRQLRRRTRKEVRAVS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: May act as a substrate-specific adapter of an E3 ubiquitin- protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins..2. The ideal protein would be one that plays a critical role in cellular response to salt stress, response to osmotic stress, regulation of protein ubiquitination, protein ubiquitination through identical protein binding within cytosol, nucleus.3. The designed protein must have at least one MATH, BTB domain.4. A protein that can modulate the overall flux of metabolites through the protein ubiquitination, Protein modification pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "CSVLQVVSSISSCKLIEEMCKRKFSMHFDYCTTASLDFVLLDQTTFSDILESADLTVTSEEKILNAVLMWCMKAEESHSWGVIDEMMNYADPKSLFKERLQSLDDLLPHVRFSLLPYELLKRLC",
    "response#2": "CSVLQVVSSISSCKLIEEMCKRKFSMHFDYCTTASLDFVLLDQTTFSDILESADLTVTSEEKILNAVLMWCMKAEESHSWGVIDEMMNYADPKSLFKERLQSLDDLLPHVRFSLLPYELLKRLL",
    "response#3": "CSVLQVVSSISSCKLIEEMCKRKFSMHFDYCTTASLDFVLLDQTTFSDILESADLTVTSEEKILNAVLMWCMKAEESHSWGVIDEMMNYADPKSLFKERLQSLDDLLPHVRFSLLPYELLKRLC"
  },
  {
    "reference": "MGAQVSKQNVGSHESGINASSGSVIKYFNINYYKDSASSGLTKQDFSQDPSKFTQPIADVLTNPALMSPSVEACGFSDRLKQITIGNSTVTTQDSLNSVLAYGEWPEYLSDLDATSVDKPTHPETSADRFYTLDSVTWGSESLGWWWKIPDCLKDMGLFGQNMFHHSMGRSGYLIHVQCNATKFHSGCLMVAVVPEHQLAYIGAGSTRVKYDHTHPGDRGHEIRQSTQWGDHQPDENPFYLCNGTLLGNVTIFPHQMINLRTNNSATIIVPYINCIPMDNMLRHNNLSLLIIPMVRLRATGTSSSTLPITVTIAPDKSEFSGAMTGQTQGLPTRIPAGGQQFMTTEDEQSPNILPEYSSTKMIHIPGKIDNILHMAMVESFIPMNNLTGHLGQVSIYNVQVPKKGSGEGQDLIVALPLQMDNTIFSTTLLGEVLNYFSNWSGSIKITFMCVCDAFSSGKFLMAYTPPGGRQPTTRKEAMLGTSVVWDLGLQSSCTMVVPWMSSTFYRSTKSDKYTSGGYITLWYQTDFIPGASGGIGVLLATCSGCPDLSVRMMRDTPMIKQPTNEIQNPVEDFVEGTLKEVLVVPNTQPSGPSHTTRPSALGAMEIGASSTATPESMIETRYVINNHTNNEALVENFLGRSSIWANLQLDNNGFKKWSINFQEQAQIRKKLEMFTYVRFDMEVTIVTNNSGLMQIMYVPPGLDTPNRVDDKLWDGASNPSVFYQPKSGFPRFTIPFTGLGSAYYMFYDGYDEGRLGSLEYGISATND",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Viral protein genome-linked: acts as a primer for viral RNA replication and remains covalently bound to viral genomic RNA; The VPg-pUpU is then used as primer on the genomic RNA poly(A) by the RNA-dependent RNA polymerase to replicate the viral genome.; VPg is uridylylated prior to priming replication into VPg-pUpU2. The designed protein should contain one or more RdRp catalytic, SF3 helicase, Peptidase C3 domains that are essential for its function.3. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.4. The ideal protein would be one that plays a critical role in suppression by virus of host gene expression, suppression by virus of host innate immune response, induction by virus of host autophagy, protein complex oligomerization, DNA-templated transcription through cysteine-type endopeptidase activity, RNA binding, metal ion binding within cytoplasmic vesicle membrane. The designed protein sequence is ",
    "response#1": "PGVSCDRFYTLPSKKWQADSKGWEWKLPDAVCDLGVFGQNSVYHFLTRCGWTIHVQANATKFHQGCLLVVAVPEHQIGSKLNPEFEDMMPGKQGRELEFAYELEDGTSIANSLIYPHQWINLRTNNSATLVIPYVNAIPMDSAIRHSNWSLLVMPVSPLAHATGTTPFVAITITLAPMFSEFSGLHPTKEISIPGEVKNLIELCQVDSMIPINNVADKIRSVDMYTVVLQRQDELMQEVFAIRVDVASQPLATTLIGEISSYYTQWTGSLRFSFMFCGTANTTLKLLIAYTPPGIAKPKSRKEAMLGTHIVWDVGLQSTISMIVPWVSASHFRNTTPDTYSLAGFITCWHPTREISIPGQVKNLVELCQIDTLIPINSVTGNTNNTSIYTVTLTRETSLAQEIFAIKVDIASDPLATTLIGEIASYYTHWTGSIRFSFLFCGSANTTLKLLIAYTPPGIRKPRNRKEAMLGTHVVWDVGLQSTISMVVPWVSASHFRNTTPDTYSLAGYVTCWHPTKEISIPGQVRNLIEMCQVDTLIPINNLEQNIRNVNMYSVVLNQETTTAKEIFAIKVDIASQPLATTMIGEIASYFTHWTGSLRFSFLFCGTANTTLKLLVAYTPPGIEKPQNRKDAMLGTHVVWDVGLQSTISMVVPWVSASHYRNTTPDTYSQAGYITCWHPTREISIPGQVKNLVELCQIDTLIPINSVTGNTNNTSIYTVTLTRETSLAQEIFAIKVDIASDPLATTLIGEIASYYTHWTGSIRFSFLFCGSANTTLKLLIAYTPPGIRKPRNRKEAMLGTHVVWDVGLQSTISMVVPWVSASHFRNTTPDTYSLAGYVTCW",
    "response#2": "PDTSSNRFYTLESKLWTNESKGWWWKLPDALKDMGVFGQNMYYHFLGRSGYTVHVQCNSSKFHQGTLIVAMIPEHQLAAAEKGNVTAGYNLTHPGERGRTIGIPDRLHPNKQPSDDNWLNFDGTLLGNITIFPHQFINLRSNNSATIIVPYVNAVPMDSMLSHNNWSLVIIPICPLEVSTTGTNGIPITVSISPMFAEFSGARTSQDVANAVVGYGVWPHYLTPEDATAIDKPTQPDTSSNRFYTLESKIWASNSKGWWWKLPDALKDMGIFGQNMYYHFLGRSGYTIHVQCNASKFHQGTLIVAAIPEHQLASASRGNVAAGYNYTHPGEIGRDVGKENREQANNQPSDDNWLNFDGTLLGNITIFPHQFINLRSNNSATLILPYVNAVPMDSMLRHNNWSLVIIPICPLGGPYIHPTKEIFIPGQVRNLSELCQVDTIVPINHTTSTTSSVNMYTIQLTKEQPGTEVFAMRVDVASQPLATTLIGEISSYYTNWTGSLRFSLMFCGSAGTTLKMLVAYTPPGIAKPETRKDAMLGTHVVWDVGLQSTMSMIVPWISASXXXXTTPDTFSQAGYITCWGPPAPGTEKPTPVIDIPGEVRNILEIVQVESLVEIQNVDGVNGVERYRIPLSVQDEMDQQIMALRVDPGTNGPLQSTLLGVFTRYYTQWSGSIEFTFMFCGTFMSTGKVVIAYTPPGGTAPANRREAMLGTHIVWDFGLQSSITLVVPWISSGHFRAADISATLFKYKYYEAGFVTMW",
    "response#3": "LIEMCQVDTLIPINNLEQNIRNVNMYSVVLNQETTTAKEIFAIKVDIASQPLATTMIGEIASYFTHWTGSLRFSFLFCGTANTTLKLLVAYTPPGIEKPQNRKDAMLGTHVVWDVGLQSTISMVVPWVSASHYRNTTPDTYSQAGYITCWYQTNLVVPPNTPTTARMLCFVSGCKDFCLRMARDTPETSADRFYTLKSVEWGATSIGWWWKLPDCLKDMGIFGQNMYYHSFGRSGFLIHVQCNATKFHTGCLIVAVIPEHQLSYVGADDVKVRYDHTHPGEDGHTFKAPTERGQWNPDENPFYLCNGTLLGNITAYPHQLINLRTNNSCTIVVPYVNAVPMDNMLRHNNLTLMIVPIVPLKPGLSGSPSLPITITIAPNKSEFSHPTKEIFIPGQVRNLSELCQVDTIVPINHTTSTTSSVNMYTIQLTKEQPGTEVFAMRVDVASQPLATTLIGEISSYYTNWTGSLRFSLMFCGSAGTTLKMLVAYTPPGIAKPETRKDAMLGTHVVWDVGLQSTMSMIVPWISASXXXXTTPDTFSQAGYITCWS"
  },
  {
    "reference": "MRVNLDRVRELDLQDPLAIFKERFALPEGVLYLDGNSLGAQPLAARDAVAETLDQWRDELIKGWNQGWFDAPERLGNRLAPVLGAGEGEVIVTDNITLNIFKLLGYIIGQRDEGRGVVLSEGGNFPTDGYIAQGFSRFGGHEHRFLPEGAPLEAHLDGVDVVVLSQVNYRTGALRDMAAVTRQVHAHGAEIIWDLAHSAGALPVDLNGCGARYAVGCTYKYLNGGPGAPAFLYVQRDHQDGGWQPLSGWHGHAAPFDFEANYAPAGDITRFRTGTHSALTYRALEASLDMWQDVDLQALRTKSLAMSELFRDCLADLLESYGITDITPAAGERGSQVSLVDTENGFAIVQALIARGVIGDYREPGLMRFGFTPLYLSFEDVWQAAGHFREVLETGEYRESRFNVRGAVT",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design must be able to perform kynureninase activity in order to contribute to tryptophan catabolic process, 'de novo' NAD biosynthetic process from tryptophan, quinolinate biosynthetic process.2. The designed protein should have a binding site that is specific to the target pyridoxal 5'-phosphate ligand.3. A protein that is capable of catalyzing reactions within the quinolinate from L-kynurenine: step 2/3, NAD(+) biosynthesis, Cofactor biosynthesis pathway with high efficiency and specificity.4. The protein should have a fast turnover rate for H2O + L-kynurenine = anthranilate + H(+) + L-alanine. The designed protein sequence is ",
    "response#1": "MKIFPRPGEHTLREEDILKVIAEQGSTIALVIFSGVQYYTGQWFPMERITKAAKAQGCICGWDLAHAVGNVPLSLHDWGVDWAVWCSYKYLNSGPGGIAGLFVHESWDDRE",
    "response#2": "MPRPGEHTLREEDILKVIAEQGSTIALVIFSGVQYYTGQWFPMERITKAAKAQGCICGWDLAHAVGNVPLSLHDWGVDWAVWCSYKYLNSGPGGIAGLFVHESWDDRF",
    "response#3": "PRPGEHTLREEDILKVIAEQGSTIALVIFSGVQYYTGQWFPMERITKAAKAQGCICGWDLAHAVGNVPLSLHDWGVDWAVWCSYKYLNSGPGGIAGLFVHESWDDRDYRLYCPSCNNFVAKFELQP"
  },
  {
    "reference": "MTQTLTETTKAIVRATVPALTTHGTEITAAMYKRLFENSKVRDLFNQSNQGEGGRQTKALAQAILAYAENIDNLAILTGAVERIAQKHVGLHIRPDHYPHVATALLGAIKDVLGEVATDDILNAWGEAFWFLANILIGREERIYDDLQSSEGGWNGWRQFAVASKRRESDIITSFVLRPVDGRAVVRHRPGQYLTFRFDIPGLPQQHRNYSISSAPNGESYRISVKREMHGLVSGFLHDQIEEGAVIEVAPPTGDFHLPPGQERPVVLLSGGVGLTPMVSMLETIANRGQRLAVHYVHGAENGSVHAMGPHVRDIAKNREHLKTTIFYGSPHDRDQKGVHFDHEGYITIDWLAKNTPMAEADFYLCGPRPFLAAFVKGLAKAGVTADRIHYEFFGPADELAAA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The FAD, NADP(+) binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must exhibit the following characteristics: Therefore, plays a central role in the inducible response to nitrosative stress.; Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds.3. The designed protein must be able to perform oxygen binding, oxygen carrier activity, nitric oxide dioxygenase activity under specific conditions.4. The Charge relay system active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "MLSQKTIEIIKSTVPVLEVHGTAITKRFYQMMFAKHPELLNIFNHANQKQGRQQAALANAVYAAALYIDKLETILPVVKQIGHKHRSLGVKAEHYPIVGENLLAAIKDVLGDAATDEILQAWAEAYGVIADAFIGIEKEMYEEAGTWQ",
    "response#2": "MLSQKTIEIIKSTVPVLEVHGTAITKRFYQMMFAKHPELLNIFNHANQKQGRQQAALANAVYAAALYIDKLETILPVVKQIGHKHRSLGVKAEHYPIVGENLLAAIKDVLGDAATDEILQAWAEAYGVIADAFIGVTAEEM",
    "response#3": "MLSQKTIEIIKSTVPVLEVHGTAITKRFYQMMFAKHPELLNIFNHANQKQGRQQAALANAVYAAALYIDKLETILPVVKQIGHKHRSLGVKAEHYPIVGENLLAAIKDVLGDAATDEILQAWAEAYGVIADAFIGIEAEMYAEAQAEAMEGTRAFRIVEKTPRSALITSFEFEPVDGQPVADYQPGQYLGVWLKPEGFPHQEIRQYSLTRKPNGKGYRIAVKREDGGQVSTWLHNEASVGDVVHLAAPAGDFFMDEEEGPLVLISGGVGLTPMISMLKKAAEGGKRVEFIHGARNSAVHAMKDWVNELAASNPNFKSYVAFSKPTEADRQAAGFDKEGYIDLEWIRGVVPDPGDYYICGPVPFMKAVVEGLKEMGIGEDRIHYEIFGPHSDLNE"
  },
  {
    "reference": "MNVYRTHLCNELREEHIDQTVTLSGWVYRKRDHGKIIFVDLRDHYGITQLVFNDSDTTIFQLITTLRLESVITIKGIVKARDSSTINETLDTGSIEVIVSSINIETASEILPINIASMQDYSEDIRLTYRFLDLRRDKVKNNIILRSKVITEIRKSMENMGFIEIQTPILTSSSPEGARDYLVPSRIHHGKFYALPQAPQLFKQLLMVSGFDKYFQIAPCFRDEDARADRSPGEFYQLDIEMSFVTQEDIFNIIEPVMINIFSKFSNKTINKEFPKISYHDAMLYYGSDKPDLRNPLVIQDVTEIFRDSEFKIFNSNIKQGMVVRAIPAPNTAHNPRSFFDSKIEFAKTLGAQGLGYITFIDDSLAKGPIAKFLDKDRLDNIKLICNIKAGDSVFFVSEIADKAALFAGEVRTLLGKELNLIEENTFKFCWVIDFPYFKYDHKEKSINFFHNPFSMPQGGLEALENQDPLNILAYQYDIVCNGIEISSGAIRNHKLNIMYKAFSIAGYTKEMVDEKFKALTRAFKFGAPPHGGIAPGIDRIVMLLADVPNIREVICFPLNQSGEDLLMGSPSEIDNDHLKLLSLNIIKKT",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be involved in aspartyl-tRNA aminoacylation by localizing to cytoplasm and exhibiting aspartate-tRNA(Asn) ligase activity, nucleic acid binding.2. The designed protein should be able to compete with endogenous proteins or inhibitors for L-aspartate, ATP binding.3. The protein should have a low Km value for ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-   aspartyl-tRNA(Asx).4. For general function, the protein need meet that Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn). The designed protein sequence is ",
    "response#1": "MRMRSEVNRIARDVLHGRDFVEVETPTMTRSTPEGARDFLIPARLQPGNWYALPQSPQLFKQLLMVGGLERYFQIARCYRDEDFRADRQPEFTQLDIEMSFVDQDDVIELGEEIISALWRETAGHEIPRPIQRMTYADAMARFGTDKPDLRFGLELTEMTEYFADTPFRVFRAPYVGAVVMPGGADQPRRQLDAWQDWAKQRGAKGLAYVLVGQDGTLSGPVAKNLSDGERDGLVKAVGAAPGDCVFFAAGDRSSSRALLGAARLEIGERCGLIDHDAWSFVWVVDAPMFESIDDTDDVAVGSGRWTAVHHPFTSPNADWVDNFESDPANALAWAYDIVCNGNEIGGGSIRIHRSDVQKRVFELLGISEEQAQDKFGFLLEAFKYGPPPHGGIAFGWDRICMLLAGADSLRDVIAFPKSNGYTEF",
    "response#2": "MRMRSEVNRIARDVLHGRDFVEVETPTMTRSTPEGARDFLIPARLQPGNWYALPQSPQLFKQLLMVGGLERYFQIARCYRDEDFRADRQPEFTQLDIEMSFVDQDDVIELGEEIISALWRETAGHEIPRPIQRMTYADAMARFGTDKPDLRFGLELTEMTEYFADTPFRVFRAPYVGAVVMPGGADQPRRQLDAWQDWAKQRGAKGLAYVLVGQDGTLSGPVAKNLSDGERDGLVKAVGAAPGDCVFFAAGDRSSSRALLGAARLEIGERCGLIDHDAWSFVWVVDAPMFESIDDTDDVAVGSGRWTAVHHPFTSPNADWVDNFESDPANALAWAYDIVCNGNEIGGGSIRIHRSDVQKRVFELLGISEEQAQDKFGFLLEAFKYGPPPHGGIAFGWDRICMLLAGADSLRDVIAFPKKGGANP",
    "response#3": "MRMRSEVNRIARDVLHGRDFVEVETPTMTRSTPEGARDFLIPARLQPGNWYALPQSPQLFKQLLMVGGLERYFQIARCYRDEDFRADRQPEFTQLDIEMSFVDQDDVIELGEEIISALWRETAGHEIPRPIQRMTYADAMARFGTDKPDLRFGLELTEMTEYFADTPFRVFRAPYVGAVVMPGGADQPRRQLDAWQDWAKQRGAKGLAYVLVGQDGTLSGPVAKNLSDGERDGLVKAVGAAPGDCVFFAAGDRSSSRALLGAARLEIGERCGLIDHDAWSFVWVVDAPMFESIDDTDDVAVGSGRWTAVHHPFTSPNADWVDNFESDPANALAWAYDIVCNGNEIGGGSIRIHRSDVQKRVFELLGISEEQAQDKFGFLLEAFKYGPPPHGGIAFGWDRICMLLAGADSLRDVIAFPKSTGTC"
  },
  {
    "reference": "MAMTVNTNVSALVAQRHLNSASEMLNQSLERLSSGNRINSAKDDAAGLQISNRLETQMRGLGIAVRNANDGISIMQTAEGAMQETTQLLQRMRDLSLQSANGSNSAAERVALQEEMAALNDELNRIAETTSFAGRKLLNGQFMKASFQIGASSGEAVQLSLRNMRSDSLEMGGFSYVAAALADKQWQVTKGKQQLNISYVNAQGENENIQIQAKEGDDIEELATYINGKTDKVSASVNEKGQLQLYIAGKETSGTLSFSGSLANELQMNLLGYEAVDNLDISSAGGAQRAVSVIDTALKYVDGHRSELGAMQNRFQHAISNLDNVHENLAASNSRIKDADYAKETTQMIKQQILQQVSTSVLAQAKRQPKFVLFLLRN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.2. The designed protein should be expressed specifically in the extracellular region The designed protein sequence is ",
    "response#1": "ATLSTTALDGAGLKTGTGSTTDTGSIKDGKVYYNSTSKNYYVEVEFTDATDQTNKGGFYKVNVADDGAVTMTAATTKEATTPTGITEVTQVQ",
    "response#2": "ATLSTTALDGAGLKTGTGSTTDTGSIKDGKVYYNSTSKNYYVEVEFTDATDQTNKGGFYKVNVADDGAVTMTAATTKEATTPTGITEVTQVI",
    "response#3": "ATLSTTALDGAGLKTGTGSTTDTGSIKDGKVYYNSTSKNYYVEVEFTDATDQTNKGGFYKVNVADDGAVTMTAATTKEATTPTGITEVTQVL"
  },
  {
    "reference": "MSKYIFVTGGVVSSLGKGLASAAIGNLLESRGLRITFLKLDPYINVDPGTMNPYQHGEVYVTDDGAETDLDLGHYERYTSLTLSRENNYTTGRIYHSVITKERRGDYLGGTVQVVPHITDEIKGSITRVAEGTDVAIVEVGGTVGDIESLPFLEAIRQMPYDVGRENVLYVHLTLVPYIGAAGELKTKPTQHSVNKLREIGIQPNILLCRTDRYLPPDIKDKIAMFCNVEKGAVITAKDVETVYEVPIVFRKEGLDELILRLLHLDTGPPNLREWDAMVQKIKHPKHEVSIALVGKYVGLKESYKSLAEALVHGGIDHETRVRINWIESEDLERKGTEQLLREADGILIPGGFGVRGIEGKITTIRYAREKSIPFFGLCLGMQCAVIELARHVAGLQGANSSEFDPHCPHPVIDLMADQRRVHDKGGTMRLGAYPCRILDGSLAHKAYGITEVRERHRHRYEFNNMYRDLLTKHGLVLSGLSPDGRLVEIVELKEHPWFLATQFHPEFTSRPHRPHPLFSAFIGAALRRKVGR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.2. The designed protein have Glutamine amidotransferase domains with specific sequence motifs that are crucial for its activity.3. The ATP binding site should be located in a region of the protein that is accessible to the ligand.4. The protein design should consider the endogenous proteins and enzymes present in the CTP from UDP: step 2/2 pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "VKIAIVGKYTGLPDAYKSLNEALVHGGIANKVRVSLDWIEAEIFEQEDPTPFLEGVHGILVPGGFGERGAEGKVAAATFARTREVPYFGICFGMQMAVIEAARNMAGIEAANSTEFGPTDEPVVGLLTEWMRGNELEQRKANGDLGGTMRLGAYEALLAGDSKIAGIYGATTIFERHRHRYEVNMRYRNLLEAAGMRFSGMSPDGLLPETVELADHPWYIGVQYHPELKSRPFEPHPLFASFVR",
    "response#2": "LHDAYLSVVEALNHAGYYHNSHVEIHWIDSEMINEANYQEKLKDLDGIIIPGGFGDRGIEGMILACKYARENNLPYFGICLGMQITVIEYARNVLGLNEADSREFNENAKDKVIDYMPGQNDDIDKGGTLRLGAYPCILKENSMIKKYYGINNISERHRHRYEFNNDYRDLFEKNGLVLSGISPDGRLVETVELSDKPFFIGVQFHPEFKSRPNRAHPLFVGFIEDSYKSLREALTHAGVANNLRVNVTWIESENLIKDGYEAELQDYDAILVPGGFGKRGIAGMLRAIKYARKSGTPYFGICLGMQTACIEFAREVCGLRDADSTEFNEQTPFPIIFKLRDLVNVEELGGTMRLGEWFCRLKEGSLAADIYHGAEEIGERHRHRYEFNPEFRTVLEKEGLTFSGVSPDGKFVEMVELSREQHPFFIACQFHPEYKSKPLAAHPLFVSFVSR",
    "response#3": "PDAYKSLNEALVHGGIANKVRVSLDWIEAEIFEQEDPTPFLEGVHGILVPGGFGERGAEGKVAAATFARTREVPYFGICFGMQMAVIEAARNMAGIEAANSTEFGPTDEPVVGLLTEWMRGNELEQRKANGDLGGTMRLGAYEALLAGDSKIAGIYGATTIFERHRHRYEVNMRYRNLLEAAGMRFSGMSPDGLLPETVELADHPWYIGVQYHPELKSRPFEPHPLFASFIS"
  },
  {
    "reference": "MSRRCDLTGKGVLSGHTVSHSNHKTKRRFLPNLCNVTLESETLKRTIRLRVSANALKSVDHRGGLDAFLKKARDEELSPRALDFKRLIAKAAAEAASTAA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be involved in translation by localizing to ribosome, ribonucleoprotein complex and exhibiting structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "LKLQKRLAASVLRCGKKKVWLDPNEINEIANTNSRQNIRKLIKDGLIIKKPVVVHSRYRVRKNTEARRKGRHCGFGKRKGTANARMPTKLLWMQRQRVLRRLLKKYRDSKKIDRHLYHDLYMKCKGNVFKNKRVLMEYIHKKKAEKQRSKMLADQAEARRQR",
    "response#2": "IPPTTALVMKEAGVEKGSGTPNTQAVGNLPLEAVIRIAKMKMESMLSYDLKTAAKEVMGTCVSVGVTF",
    "response#3": "IPPTTALVMKEAGVEKGSGTPNTQAVGNLPLEAVIRIAKMKMESMLSYDLKTAAKEVMGTCVSVGVTF"
  },
  {
    "reference": "MKLFQKIFGSYSQREVKRIMPIVDKIDALDSKVQALSNEQLRNKTDEFKERLDKGESLDSILPEAFAVVREVGFRTVGLKQYREQLIGGIVIHQGRIAEMKTGEGKTLVATAPAYLNALTGKGVHIVTVNDYLAKRDRDTMAPIYEALGLKVGVILHDMSQSQRQEAYNCDITYGTNSEFGFDYLRDNMVIYKEERVQRKLNFAIVDEVDSILIDEARTPLIISGEGEKSTEFYNIANGFAKSLEKEDYKVDEKANAVMLNDTGIKKAETFFSLENYADPENMEIQHYVVQALKANYIMKRDKDYMVKNGEVLIVDEFTGRMMEGRRYSDGLHQAIEAKEGVKVERESKTLATITYQNYFRIFNKLSGMTGTAQTEENEFRHIYGLDVIVVPTHKPIAREDFPDVVYKSAKGKFKAIADEIYETYKKGQPALVGTVSIEKSELLSDMLKRKGVPHQVLNAKFHEKEADIISYAGQKGTVTIATNMAGRGTDIKLGKGVVALGGLKIIGTERHESRRIDNQLRGRSGRQGDPGMSRFYVSLEDDLMRIFGSDRLQGIVEKLGLKDDEAIESKMVSNAIENAQKKVEGNNFDIRKTLLQYDDVINKQREIIYKQRSQVLEGEDLKNDVQDMIKSLINSIVDSHISGIEEEFEDEIVKLIEYMEDVYVPKDSVKKEDIINLSNEAIKDKFIDIAQKIYEQKEIEFTSEQMREIERVILLRVVDTRWMDHIDDMEHLKRAIGLRAYRQQEPAQAYQFEGSEMFEEMIYNIKL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.2. For general function, the protein need meet that Interacts with the SecYEG preprotein conducting channel; Part of the Sec protein translocase complex; Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.3. The designed protein should have a high specificity for Zn(2+), ATP binding, with minimal off-target effects.4. The protein have enhanced ATP + H2O + cellular proteinSide 1 = ADP + phosphate +   cellular proteinSide 2..5. The designed protein should be involved in protein import, protein targeting by localizing to cytoplasm, plasma membrane and exhibiting ATP binding, protein-exporting ATPase activity. The designed protein sequence is ",
    "response#1": "MGGIFLHQGNIAEMKTGEGKTLVATFPAYLNALAGKGVHVVTVNDYLAKRDAEWMGKVYAQLGLATGVVYPFQSDEEKKAAYAADITYATNNELGFDYLRDNMKASKEEMRQRGHFFAIVDEVDSILIDEARTPLIISGPSQDRSDLYTKVDKLIPELEYIFDRAQAEDSSQLYLQINALIPRLKRHIEEEEGVVTQEGHYVVDEKTRQIELNEQGHQFIEELLASAGLLPEGDNLYSAHNLQLLTHVYAGLRAHVLFHRNVEYIVQGNQVLLIDEHTGRTMQGRRLSEGLHQAIQAEDSSQLYLQINALIPRLKRHIEEEEGVVTQEGHYVVDEKTRQIELNEQGHQFIEELLASAGLLPEGDNLYSAHNLQLLTHVYAGLRAHVLFHRNVEYIVQGNQVLLIDEHTGRTMQGRRLSEGLHQAIQAEDSSQLYLQINALIPRLKRHIEEEEGVVTQEGHYVVDEKTRQIELNEQGHQFIEELLASAGLLPEGDNLYSAHNLQLLTHVYAGLRAHVLFHRNVEYIVQGNQVLLIDEHTGRTMQGRRLSEGLHQAIQAEDSSQLYLQINALIPRLKRHIEEEEGVVTQEGHYVVDEKTRQIELNEQGHQFIEELLASAGLLPEGDNLYSAHNLQLLTHVYAGLRAHVLFHRNVEYIVQGNQVLLIDEHTGRTMQGRRLSEGLHQAIQAEDSSQLYLQINALIPRLKRHIEEEEGVVTQEGHYVVDEKTRQIELNEQGHQFIEELLASAGLLPEGDNLYSAHNLQLLTHVYAGLRAHVLFHRNVEYIVQGNQVLLIDEHTGRTMQGRRLSEGLHQAILAEEASAQIYQAINHMIPHFTKQIESGAGKEIVIEVAGDYTVDEKHKQVFLTDNGHGKAERLLIDAGALLEGVSLYDASNILLMQHINSALRAHILFQKNVDYIIQNDEVVIVDEFTGRTMLGRRWSEGLHQAI",
    "response#2": "MGGIFLHQGNIAEMKTGEGKTLVATFPAYLNALAGKGVHVVTVNDYLAKRDAEWMGKVYAQLGLATGVVYPFQSDEEKKAAYAADITYATNNELGFDYLRDNMKASKEEMRQRGHFFAIVDEVDSILIDEARTPLIISGPSQDRSDLYTKVDKLIPELTYFDEKSPSEDSSFLYKEINKLVPSLICQKKEDSDKFHGNGHFSIDEKSKQIYLTERGLVEVEKILLDRKLMKKEESLYSSNNIILMHHVISALRAHNLFTRNIDYLVKDNNIIIVDEHTGRTMPGRRWSDGLHQAIQAEDSSQLYLQINALIPRLKRHIEEEEGVVTQEGHYVVDEKTRQIELNEQGHQFIEELLASAGLLPEGDNLYSAHNLQLLTHVYAGLRAHVLFHRNVEYIVQGNQVLLIDEHTGRTMQGRRLSEGLHQAIRSDLYTKVDKLIPELVEEHYKLDEKTRNVTFTEEGNEFLEKRLLETGLLPEGQSLYDPESTTIVHHVNQGLRAHKLFNRDQQYIVRDDEIMLIDEFTGRMMRGRRLSDGLHQAISSQLYLQINALIPRLKRHIEEEEGVVTQEGHYVVDEKTRQIELNEQGHQFIEELLASAGLLPEGDNLYSAHNLQLLTHVYAGLRAHVLFHRNVEYIVQGNQVLLIDEHTGRTMQGRRLSEGLHQAIQAEDSSQLYLQINALIPRLKRHIEEEEGVVTQEGHYVVDEKTRQIELNEQGHQFIEELLASAGLLPEGDNLYSAHNLQLLTHVYAGLRAHVLFHRNVEYIVQGNQVLLIDEHTGRTMQGRRLSEGLHQAILATENLSSELYIKVNKIIPNLIRQEKEDSDSFQGEGHFSVDEKARQVHLTERGLVAVEELMVSEGIMAEGESLYSPGNIMMMHHVTAALRAHVLFTRDVDYIVKDGEVIIVDEHTGRTMQGRRWSDGLHQAV",
    "response#3": "MGGIFLHQGNIAEMKTGEGKTLVATFPAYLNALAGKGVHVVTVNDYLAKRDAEWMGKVYAQLGLATGVVYPFQSDEEKKAAYAADITYATNNELGFDYLRDNMKASKEEMRQRGHFFAIVDEVDSILIDEARTPLIISGPSQDRSDLYTKVDKLIPELEAAKRLIDQNDLIYKTEKEKFDAVIAQIKYLHAKGQPVLVGTASIEKSEKLHELLTKEKINHSVLNAKNHEKEAQIIANAGDKGAVTIATNMAGRGVDIKINDEVRALGGLYILGTERHESRRIDNQLRGRSGRQGDPGESRFYLSLEDNLLRIFGSDKIKHIMERLGLKEGESIESRLVTKAVENAQKKVLAPIEHKLISRAIENAQKRVEGQNFDIRKHLLEYDDVMNRQRQVIYEQRRNVLKGDELREDLLDMIEEVVEDFVPEYVDEKRHPDEWNLKGLEDRVLKQFSLRLDFSKSGDVGSLEDIQEKIVAAVNDLLNRKEAEFGKPLMDYLIRMISIQSIDSHWKDHLLAMDHLKEGIGLRGYGQKDPVREYQKEGYDLFMDMIRRVIQSKMFTRQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYAQRQEVIMEENDLSDVLMGMVKRTIGRVVDSHTQLEKEEWNLDGIVDFAASTLVHEDTISKKDLENKSAEEIKDYLVARAQEVFEEKSQQLNGQEQLLEFEKVVILRVVDTKWTDHIDAMDQLRQSVGLRAYGQNNPLVEYQTEGYSMYNNMVGSIRAKSRPQAEDSSQLYLQINALIPRLKRHIEEEEGVVTQEGHYVVDEKTRQIELNEQGHQFIEELLASAGLLPEGDNLYSAHNLQLLTHVYAGLRAHVLFHRNVEYIVQGNQVLLIDEHTGRTMQGRRLSEGLHQAI"
  },
  {
    "reference": "MTLAGKRILLVIAGGIAAYKSLDLIRRLRERGASVRPIMTRGAQEFITPLAVGALSASHVYTELFSREDEQDVGHIRLARECDLVLVAPATADLMAKMANGLADDLASTVLLATDRPVLVTPAMNPKMWAHPATQRNVAVLQGDGIQMIGPMEGEMAESGERGRGRMAEPLDIVAGVEDLLSGPKPLAGMTAVVTSGPTHEPIDPVRYIANRSSGKQGHAIAAALARLGAEVTLVSGPVTIPDPAGVRTLHVERAEEMRDAVLGALPADIAVMVAAVADWRVASSAGQKIKKQPGEAPPPLELAENPDILKTVGHHAQRPRLVVGFAAETQNVEENGRSKLERKGADLIVANDVSPETGIMGGDRNRVKLISRDGVEGWPDLSKDEVAERLALWIATQIGGTGA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should take into account the physiological conditions of the coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway.2. A protein with phosphopantothenate--cysteine ligase activity, metal ion binding, FMN binding functions is required to enable pantothenate catabolic process, coenzyme A biosynthetic process.3. The protein should be able to bind CTP ligand in a variety of conditions4. The protein should be designed to have high stability and activity towards H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.5. The presence of DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.6. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.7. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A The designed protein sequence is ",
    "response#1": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALAVLAPTVAGDTPLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHLVREDGTTEELPRGPKEDLADAVWDLVVARLPED",
    "response#2": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALAAAEALAAQALAGRRVVVTAGGTREPLDPVRYISNYSSGKMGFAIAAAAADRGARVALVTTAAHPAHQGIDVRPVETADEMLAELKRDLAGADLLVMAAAVADFRPARIADDKIRREDTSKLTLELEQVPDLVAALGKEKEGQRVFRVGFAAEGSDLAAKGLEKMKKKGLQAIVANDISRKDIGFGSDHNEGVLPDLVLGDSTGTLLTDDDSAAAAADAAEAAEAADAAEGGAEAAAPPPEQASEEPARAAEAAPAPEQASPAPGTAAPAPAPAPAPEDDAPPVPAAAADPAAAADPAAAAGPAAAAGPAAAAGPGAATRPGLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHLVDAAGTELPTGSKREVADALWDAVVRQLDAR",
    "response#3": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALALAARLLAPRPLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHVLSADGTAVDVTGTKAEVADAVLDVVLRARGGRA"
  },
  {
    "reference": "MAKQAKAGAARRPQRGRRRERKNVPRGQAHVQATFNNTIVTITDPAGNVVCWSSAGASGFKGSRKSTPYAAQVTAEQAARKAMDNGMRVVEVYVKGPGAGRESAVRALQATGLSVIAITDVTPIPHNGCRPPKRRRV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should emphasize Basic residues compositional bias.2. For general function, the protein need meet that Forms part of the Shine- Dalgarno cleft in the 70S ribosome.3. The designed protein should be involved in translation by localizing to ribosome and exhibiting rRNA binding, structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "MPSKPGNNLPLKNIPTGTVVHAIELRPLGGAKIARSAGAAVQLVAKDGAYAQLRMPSGEIRNVDARCRATVGEVGNEDHANIQLGKAGRARWIGRRPITRGESMNPVDHPHGGRTRGGKPPVSPWR",
    "response#2": "MMGRNSNGRITTRHRGGGHKQRYRQIDFKRNKTGVPGKVERLEYDPNRSANIALVLYQDGERRYIIAPRGVEPGSPH",
    "response#3": "MSSKPGNNLPLKNIPTGTVVHAIELRPLGGAKIARSAGAAVQLVAKDGAYAQLRMPSGEIRNVDARCRATVGEVGNEDHANIQLGKAGRARWIGRRPITRGESMNPVDHPHGGRTRGGKPPVSPWR"
  },
  {
    "reference": "MERLIFYITLITFIFLLFLYPIFIKWLKKRQFGQYIRKEGPDLHNYKQGTPTMGGILFILAIFFLSLLTYFIQKEDLFLIIGVASLLFGFIGFLDDYLSIKKKDSTGLTAIQKLLLQFLFSIVIVYLISIFNNHSTLKIPFTTKSLDLKFFYPLWGIIYLTGMSNATNLTDGIDGLSGGIYVISALFTALIAGINFNHIPLLILPVIAYLFYNIKPAKIFMGDTGSLALGGILGSLALYYSVELFTILTCFIFISEMFSVIIQVGSFKVRKKRVFLMAPIHHHFELKKWSEERIVLIFWTINILTGIVALGGVL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in plasma membrane that is critical for cell cycle, cell division, cell wall organization.2. The protein must exhibit the following characteristics: Catalyzes the initial step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan: transfers peptidoglycan precursor phospho-MurNAc-pentapeptide from UDP-MurNAc- pentapeptide onto the lipid carrier undecaprenyl phosphate, yielding undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide, known as lipid I..3. The protein should have a Helical domain that is long enough to span the entire membrane.4. The protein should be designed to have high stability and activity towards di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-alpha-   D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-   alanyl-D-alanine = di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-   alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-   alanyl-D-alanine + UMP. The designed protein sequence is ",
    "response#1": "MQLSELLKGFPIKEITGNPDLDVTEVTHDSRRAGPGFLFVAIKGFRVDGHRFVPEVLARGAAAVVSEREVSLPGIVLVQVADSRRALSLIAANFYGNPAEKLKLIA",
    "response#2": "MKLSELLKGIDYQLVKGDTATDVLSVGVNDDSRQVNAGCLFVCIKGAKFDSHDHLQDVIAKGVTVILSEQPIDLNGVTQVIVPDTLRAMSLMAANYFGEPTKQLTMVAKAGTPTMGGASAKAGTPTMGGYDRRWVSLDEIAPVLVQSVMMSEDGQYCFHGGVDWAEMRMLVEDTLKGQATRGGSTIPMQTAKNLFLWNSRSFVRKAMELPLAVSTDFVLSKRRLMEIYLNIAEWGPGIYGIEAAAQHHFKVPASKLTRRQASLLAVSLPNPIDRNAGKPGRGLEAADMLYDRRWVSLDEIAPVLVQSVMMSEDGQYCFHGGVDWAEMRMLVEDTLKGQATRGGSTIPMQTAKNLFLWNSRSFVRKAMELPLAVSTDFVLSKRRLMEIYLNIAEWGPGIYGIEAAAQHHFKVPASKLTRRQASLLAVSLPNPIDRNAGKPGRGLVG",
    "response#3": "MQLTSLAKATGAPLTGPDPAAALSAXAXDARAXGTAXDXRPAPSAPPASSAPAHAAXAAADALXTTVFGDPXSGXAAXGAXATGTXAXGAXAXDXPVXAXGXPXXAAVWGAXDAXPXGXPAPXVXGXVFXGXQTAFSXSXAXGXDIXGLXAPXPAGXVNXTKAGTPTMGGVNAVNLTDGLDGQXTXSXAPXXAXSAYDRRWVSLDEIAPVLVQSVMMSEDGQYCFHGGVDWAEMRMLVEDTLKGQATRGGSTIPMQTAKNLFLWNSRSFVRKAMELPLAVSTDFVLSKRRLMEIYLNIAEWGPGIYGIEAAAQHHFKVPASKLTRRQASLLAVSLPNPIDRNAGKPGRGLXVTXGXEAXGXGXDXPSXPPDTXPTPXPFAAXGXPRXAAVXAAAANAVNFTDGLDGXAQXCYDRRWVSLDEIAPVLVQSVMMSEDGQYCFHGGVDWAEMRMLVEDTLKGQATRGGSTIPMQTAKNLFLWNSRSFVRKAMELPLAVSTDFVLSKRRLMEIYLNIAEWGPGIYGIEAAAQHHFKVPASKLTRRQASLLAVSLPNPIDRNAGKPGRGL"
  },
  {
    "reference": "MWLLLPILLYSAVFLSVRHIYSHWRRRGFPSEKAGITWSFLQKAYRREFRHVEAICEAYQSGKDRLLGIYCFFRPVLLVRNVELAQTILQQSNGHFSELKWDYISGYRRFNLLEKLAPMFGTKRLSEMFGQVQKVGDHLIHHLLDRQGQGCPQEVDIQQKLRVYSVNIIANLIYGLDINNFEHEDHILTSYLSHSQASIQSFTLGRLPQKSSYTYRLRDLIKQSVELREDHGLIRKDILQLLVRFRNGNEVSGDKWQLEPINDADKLLSIKRLAKVAEDLLKVSLDAVASTVTFTLLEILQEPLIVEKLRAEIKELSNENGQLKFEELNGLRYMDMCLKETLRKYPPLPIIERVCRKSYSLPNSKFTIDEGKTLMVPLLAMHRDEKYFSEPMKYKPLRFLQTANDVGQCEDKTKSNVFIGFGIGGSQCVGQNFAKLVIKVALIKLLQNFHLELDANQVKTLKVSHRPAPFIHTKDGLKVKLKRREINTKFYS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein can be targeted to endoplasmic reticulum membrane and perform monooxygenase activity. The designed protein sequence is ",
    "response#1": "QLLLESQSQLDEAKSEAQKQSDELALVRQQLSDMRSHVEDGDVAGSPAVPPAEQDPMKLKTQLERTEATLEAEQTRRQKLTAEFEEAQRTACRIQEELEKLRAAGPLESSGKEEITQLKERLEKEKL",
    "response#2": "NYVMHQTLSTYYYFMKPSLAPSKVMDVFIKAAEYMEMPVRRSDDEPLQKLFVAVRSELNLDLKNIRTEQAKFWKQHPSLVKMELLIQAHLTGESFALTPALLKDYRHMLELAPRLLDELVKIALLPRSPHGFGWLRPAIGVIELSQNIIQAVPLSARKASGGNSEGIAPFLQLPHFTETVVKKIARKKIRSFQEFCDMPVEERATLLTQVAGLSDEGAQDVELVLEMIPSIEVDIKCETEGEEGIQEGDVVTMYAWVSLHRRNGLTAALPHAPSFPFHKEENFWLLLADAASNEVWLSQKVSFMDETTAITAASKAIQETQEALGASAKEIGIAIREAVDRVKKGSRLVMGKFQAPAEGTHNLTSFCLCDAWIGCDTKTSC",
    "response#3": "QLLLESQSQLDEAKSEAQKQSDELALVRQQLSDMRSHVEDGDVAGSPAVPPAEQDPMKLKTQLERTEATLEAEQTRRQKLTAEFEEAQRTACRIQEELEKLRAAGPLESSGKEEITQLKERLEKEKQ"
  },
  {
    "reference": "MPLGLFSSGKAQVLCDEKIPGGKKKEPKQLSENKCKGVTLKLDHTRVTIEKQIAEGGFAIVYVASDRKNNKFALKRQFTKDNEKQLEACCREHSFLKQCIGHKNIVEFVDSYTNCLGNGIWECMLLTEYHQKNVLQLMNERISQNQYLTNDEILSIFTDLCEAVSFIHNRPQPIIHRDLKVENVLISSHKPPHYVLCDFGSATTQILSVEKYGVEYVKSEVERNTTMCYRSPEMIDFYSGLEIGLKSDIWALGVLLYRLCFFCVPFEESPLAIQSVNYQFPSVPNIPDEIKVLIYMLLDIDVNRRPSIYQTSVLAFEANHRKPLSEEIQNKKCTDAVPSLKSCIQLMRDGSNPRNKRDSSPRNPEAPPIQSSSKMASLSQQVPSISNISMPSGSGTVETSVAPRLRPKATTVVPNVPSISPVPPVGLPHLRLPSKGSTDETDGSQVRKVPIDFHHRQSFSGEEQLKPAAEADSAGPLSCPLIKPTDLGFTDLDKPALPRDRAQTDGKRRLPHESDIIFQQQHRRNVSDTSQISRSAFKPYSSQQTTSKTSSQVVRSVEDMSQRQNGGSGEWNPFLVAPFSNNSISRKDGQESAFMMDDSHFGMVFDEIRRKEIPAELDSETSSIDSRDPFGAAPFDQLTVSTSSSAQPVSLPPATDEDDERQLLSETDEEEKYEIDEKEEIQTKKDETINEEDSEIDEQRMNDRRRYSYENIDGVGDDASSDSRGKTDRDDSEEEEDDDSRRGGDTSHDEDSQNTVGSEDGEGGSRPL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Prioritize Pro residues, Acidic residues, Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.2. The protein should be designed to have high stability and activity towards ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-   threonyl-[protein].3. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.4. The protein must have high specificity towards protein serine kinase activity. The designed protein sequence is ",
    "response#1": "YEMGRLLGKGTFGKVYYGKEITTGESVAIKIINKDQVKREGMMEQIKREISIMRLVRHPNIVELKEVMATKTKIFFIMEYVKGGELFSKIVKGKLKEDSARKYFQQLISAVDFCHSRGVSHRDLKPENLLVDENGDLKVSDFGLSALPEQILQDGLLHTQCGTPAYVAPEVLRKKGYDGAKGDIWSCGIILYVLLAGFLPFQDENLMKMYRKIFKSEFEYPPWFSPESKRLISKLLVVDPNKRISIPAIMRTPWL",
    "response#2": "GEIPKFGGAGSYGEVQIFKQTGLAIKTASSRSCFEHELAVSLLTGECSLRAQASLGIGGIICLMAFSLPSKQMVFPAYDADLNAYGYRLSRSGPPSVLVTESIERAFIGLGRALVYLNTSCGLTHLDVKGGNIFVNHSHFVISDCVIGDLSLMTLNTNSMAMRAEFEIDTGEEEIKTLRLPRSASQMTFSFVIGHGLNQPISVIADFINNSGLAKSTGPIKHDVGLTIDLYALGQALLELLLVGCISPCLSVPILRTATYYYYSNKLSVDYALDLLAYRCSLYPALFPTTPLTTIYGIPWDQVEGVFESIAGAHHREAFRAHLERYRLTHRRLFASIRIPSAFTGVLELVSLLCHANEKARLSIPLLWTPRS",
    "response#3": "GEIPKFGGAGSYGEVQIFKQTGLAIKTASSRSCFEHELAVSLLTGECSLRAQASLGIGGIICLMAFSLPSKQMVFPAYDADLNAYGYRLSRSGPPSVLVTESIERAFIGLGRALVYLNTSCGLTHLDVKGGNIFVNHSHFVISDCVIGDLSLMTLNTNSMAMRAEFEIDTGEEEIKTLRLPRSASQMTFSFVIGHGLNQPISVIADFINNSGLAKSTGPIKHDVGLTIDLYALGQALLELLLVGCISPCLSVPILRTATYYYYSNKLSVDYALDLLAYRCSLYPALFPTTPLTTIYGIPWDQVEGVFESIAGAHHREAFRAHLERYRLTHRRLFASIRIPSAFTGVLELVSLLCHANEKARLSIPLLWTPRR"
  },
  {
    "reference": "MSKARLRKGRALTGVVLLNKPQGMSSNHALQRVKRLYNAQKAGHTGALDPLATGILPVCLGEATKFSQYLLDADKAYRVEATLGVRTTTSDAEGEVVEEKPVAVDTAKVADAIKQFIGEQDQSPSIYSALKHEGRPLYYYARQGIEVPKKTRTITVHSIELLNIQDNKVTLQVSCSKGTYIRTLVDDLGQLLGCGAHVSMLHRNAVADIAEAAMVTLEQLETLAEEGYEGLDALLHPADLLLGQLPEVTVTQAQTRDFLHGQPIPLPEQNGNDAEEWRVATENSLFLGVARVKNAELWPRRVIAREHVDL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must have high specificity towards RNA binding. The designed protein sequence is ",
    "response#1": "YDLEANLAVLKLYQFNLAYFQTTVTAQILLKALTNLPHTDFTLCKCMIDQTHQEERPIRQILYLGNLLETCHFQSFWASLEENRDLIDGITGFEESVRKFICHVVGITYQNIEYRLLAEMLGDPLDTQVKVWMNKYGWTENEDGQIFIHNQEESVKPKNIVEC",
    "response#2": "KAEYFIIKALENGVNVMGLTRGHDTRFHHTEKLDQGEVMIAQFTEHTSAIKVRGKALIQTSHGEITNN",
    "response#3": "YDLEANLAVLKLYQFNLAYFQTTVTAQILLKALTNLPHTDFTLCKCMIDQTHQEERPIRQILYLGNLLETCHFQSFWASLEENRDLIDGITGFEESVRKFICHVVGITYQNIEYRLLAEMLGDPLDTQVKVWMNKYGWTENEDGQIFIHNQEESVKPKNIVEC"
  },
  {
    "reference": "MKIVLASGNKGKIKEFEKLMPNDEVVAFKEILGDIEIIEDQNSFKGNAIKKAQTIYDKLLEKGFSDIIVISDDSGITVPALNNEPGIYSARYAGVNASDKENNAKLIKNLNLKTLEKTPAFYTACIAIVYQGEVYTVHGWMHGNVINEQKGEGGFGYDPMFIANGFDKTLGELGYEAKKDFSHRTKALNLAKKVLDVII",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.2. The protein should have a high affinity for Mg(2+), substrate.3. The designed protein should have metal ion binding, nucleotide binding, dITP diphosphatase activity, ITP diphosphatase activity, ribonucleoside triphosphate phosphatase activity to facilitate purine nucleoside triphosphate catabolic process, nucleotide metabolic process.4. The protein should have a fast turnover rate for dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MKLVLASNNAGKLKEFQQLLKPAGIELIPQGDLGVPEAEEPHVTFVENALAKARHASAATGLPALADDSGICVDALGGAPGLYSARYAGDHDDAANNAKLLHELAGVPEERRTAHFYCVIVLMRHPADPAPVIAEGTWEGRITTAPQGAGGFGYDPLFWLPELDQSSAQLPKEVKNTRSHRGKALDALLAKLR",
    "response#2": "MINPQTHFGFREVPVAEKAGRVRGVFDSVAARXXDXGWXRFXRXLAVXSGMRHGXRVLDLAGGTGDLALAFAPRVGPEGEVLLXDXNXMMLTAGREXLLDRGVAXGVSYVQADAERLPFADGXFDVISVAFGLRNMTHKDAALAEMLRVLKPXGRLLVLEFARPQAWPLRKAYDWYSFRVLPMMGRMVAGDADSYRYLAESIRMHPDQETLRGMMTEAGFVECDYHDLTGGIVALHRGXKA",
    "response#3": "MKLVLASNNPGKVREIAAIMAADGLEVVPQGELGVPEAEEPHRTFVENALAKARHASRISGLPAIADDSGLVVDALDGAPGIYSARYAGEGASDAENNRKLLAALAGVPDERRTAHFYCVLVYMRHGDDPAPVIAEGVWHGEIAAEPRGAGGFGYDPLFHVPAFGCSAAELTPEQKNAISHRGQALRALLERLKAR"
  },
  {
    "reference": "MVSMSTTGNAHTENVQHRLTVETVEIAPNTTAIRCLDWDRDRFDIEFGLQNGTTYNSYLIRGEQTVLVDTSHQKFRQLYLETLKGLINPKAIDYIIVSHTEPDHSGLVEDVLQLAPRATVLASKIALQFLEGLVHDPFSKRIVKSGDRIDIGKGHEIEFVSAPNLHWPDTIFSYDRKTEVIYTCDAFGMHFCDNRTFDEDLEAIEADFRFYYDCLMGPNARSLLNAMKRMGDLGKIKIIANGHGPLLYHHLDVLTECYQSWSQRQAKSETTVGLFYVADYGYSNLLVQAIGEGIQKTGVAVEMIDLSTAEIQEIQELAGRAAGLIIGMPPTTSVAAQAGISSLLSVVKDKQAVGLFECFGGDDEPVDTIRRKFIDLGVKEAFPAIRIKDVPGASAYQLCTEAGTDLGQLLTRERNIKQIKSLDVNMEKALGRISNGLYIVTTKKGDVSSAMLASWVSQASLQPLGFTIAVAKDRAIDSLMQVGDRFVLNVLEEGNYQELKKQFLKRLHPGADRFAGVRTQTAKNGSPILTDALAYMECEIQSSLECSDHYILYCTVEDGRVSKPDGLTAVRHRKVGNYY",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must be engineered to have a cooperative binding mechanism with Fe cation to ensure proper regulation. The designed protein sequence is ",
    "response#1": "TRQHAQHKGSFGDVLVVGGSHGMAGAAVLAARAAHAAGAGRVHVDLLDDNAFTHDLVRPELMFRPSERRAALPASATVVAGCGGGDPIAAALPRLLTVSERLVLDADALNALATDPTLQAALTARGGLGLGTVLTPHPLEAARLLGTDTATVQRDRLAAALQLARRFACVVVLKGSGSVVASPDTAPAINPTGNAALATAGTGDVLAGWIAGLWAAHGLTAVEAAERGTWQHGEAADRR",
    "response#2": "KGLHMICHYYPPCPQPDQTLGTSKHSDNTFITILLQDNIGGLQILHQDCWVDVSPLPGALIINIGDFLQLMTNDKFISVDHRVLTNRVGPRISIACFFSG",
    "response#3": "VLVVGGSHGMAGAAVLAARAAHAAGAGRVHVDLLDDNAFTHDLVRPELMFRPSERRAALPASATVVAGCGGGDPIAAALPRLLTVSERLVLDADALNALATDPTLQAALTARGGLGLGTVLTPHPLEAARLLGTDTATVQRDRLAAALQLARRFACVVVLKGSGSVVASPDTAPAINPTGNAALATAGTGDVLAGWIAGLWAAHGLTAVEAAERGTWQHGEAADRSPLTPLR"
  },
  {
    "reference": "MARTHDDKWDLASSVGATATIVAAGRAMASRDPRGLIDDPFAEPLVRAVGVDFFIKMMDGEFDLSVLQNVSSAKAQAMVDGMAVRTKYFDDYFGDAIKSGIRQAVILASGLDARAYRLPWPADTVVYELDQPQVIEFKTNVLADLGAEPRATRRAIPIDLRGDWPVALRAAGLDTTAPTAWLAEGLLIYLPPEAQDRLFDNITALSAPGSTVATEFVPGIVDFDVDRARQMSGPFRDHGLDIDMSSLVYTGARNHVVDYLRAKGWDAEGVTRSKLFERNGMAVPAPSDDDPLGEIIFISAALTG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity.. The designed protein sequence is ",
    "response#1": "LSSGW",
    "response#2": "ILDVGCGTGELAVRLAKEGFLVTGVDLSENMLAIAQAKAEAQQVTIEFFQQNMTELEGFSSFDCVTIFCDSLNYLLEESEVRQTFSRIYELLKEDQ",
    "response#3": "ILDVGCGTGELAVRLAKEGFLVTGVDLSENMLAIAQAKAEAQQVTIEFFQQNMTELEGFSSFDCVTIFCDSLNYLLEESEVRQTFSRIYELLKEDE"
  },
  {
    "reference": "MRVKGIRKNCQHLWRGDTLLLWRGGILLLGILMTCSAADQLWVTVYYGVPVWKEATTTLFCASDAKAYDKEVHNVWATHACVPTDPNPQELVLGNVTENFNAWKNNMVEQMHEDIISLWDQSLKPCVKLTPPCVTLNCTDFKKNDTNSNTTKANTTSPNTNGSSWEMMEEGEIKNCSFNVTAGIRDKMQKEYALFYKLDVTPINNSNTSYRMISCNTSIITQACPKITFEPIPIHYCAPAGFAILKCNDEKFNGTGPCRNVSTVQCTHGIKPVVSTQLLLNGSLAEGEVVLRSKNFSNNADTIIVQLKEPVVINCTRPNNNTRKSINIGPGRAFYTTGQIIGNIRQAHCNLSRAQWNNTLRQVARKLREQFNNKTIVFNHSSGGDLEIVMHTFNCGGEFFYCNTTQLFASTWDENNITANSTENDNVNITLPCRIKQIINMWQEVGKAIYAPPISGQIRRSSNITGLLLMRDGGNTNITNSPNKTATEVFRPGGGNMKDNWRSELYKYKVVEIEPLGAAPTKAKRRVVQREKRAGLGMLLIGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQNNLLMAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNNSWSKNKTLTDIWDNMTWMEWEREINNYTGIIYNLLEKSQNQQEKNEQELLALDKWANLWSWFDITNWLWYLKIFIMIVGGLIGLRIIFTVLSIVNRVRQGYSPLSLQTLLPTQRGPDRPGGIEEEGGERDRDRSG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein's functional design necessitates the inclusion of a YXXL motif; contains endocytosis signal motif.2. A protein with structural molecule activity is needed to enhance positive regulation of establishment of T cell polarity in a specific context.3. For general function, the protein need meet that Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state4. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "MGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQRFLGLWGCSGKIICTTAVPWNSTWSNKTFDQIWKNMTWTQWEREISNYTNQIYDILTESQNQQDRNEKDLLELDKWASLWNWFDITNWLWYIRIFIMIVGGLVGLRIIFAVLSIVNRVRQGYSPLSFQIR",
    "response#2": "MGAASMTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNTSWSNKSQEDIWGNMTWMQWDKEISNYTDTIYQLLEESQNQQEKNEKDLLALDKWQNLWSWFNITNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTR",
    "response#3": "MGAASITLTVQARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARLLAVERYLRDQQLLGLWGCSGKLICTTTVPWNSSWSNKTQKEIWENMTWMEWEKEISNYSNEIYRLIEEAQNQQEKNEQELLALDKWASLWNWFSITNWLWYIKLFIIIVGGFIGLRIVFAVLSIVNRVRKGYSPLSLQTN"
  },
  {
    "reference": "MPKKPPQRSTRKSTVKATRRKTAAVDPRKYSRLVVDGVKQTPARAMLRAVGFGDEDFLRPQVGIASTWSNLTPCNMHIDELARIAAAGVEAHGAKAVTFGTITVSDGISMGTPGMRYSLVSREVIADSIETVVGAQGFDGIVALGGCDKNMPGCLMALARLNRPAVFVYGGTIRPGAEGRDIVSVFEAIGGYAGGAVSEQELKYVERTAIPGPGSCAGMYTANTMASAIEALGMSLPNSSAQDAVSTEKREDCARAGEAVVRLIERGLKPRQIMTRAAFENAITVAIALGGSTNVVLHLLAIASSANVKLRLTDFARIGKKVPVLADLRPSGRYSMSELVAIGGIQPLMKTLLDAGMLHGDCLTVTGQTLAENLVDVAPYPADQQIVRPLSDPLKKDSHLVVLHGNLAPDGAVAKISGKEGLRFEGLARVFDSEEKALDGILGGAVRAGDVVVIRYEGPKGGPGMREMLSPTGAIMGKGLGKQVALITDGRFSGGSHGFVVGHVSPEAAVGGPIGLVRDGDRISIDARRRAMTLHITDAELRRRRKAWRAPKPYARRGALMKYAKCVSSATLGAVTDLE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should exhibit Basic residues compositional bias.2. The designed protein must possess dihydroxy-acid dehydratase activity, 2 iron, 2 sulfur cluster binding to support valine biosynthetic process under certain conditions.3. The protein must exhibit the following characteristics: Functions in the biosynthesis of branched-chain amino acids; Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo- 3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3- dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively..4. The protein must bind to [2Fe-2S] cluster in order to perform its enzymatic function.5. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for (2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-   oxopentanoate + H2O.6. The designed protein should be able to compete with endogenous proteins or inhibitors for [2Fe-2S] cluster, Mg(2+) binding. The designed protein sequence is ",
    "response#1": "MPKPIIGIANTWIEIGPCNLHLRELAEHIKQGVREAGGTPMEFNTVSISDGITMGSEGMKASLVSREVIADSIELVARGNLFDGLIALSGCDKTIPGTIMALERLDIPGLMLYGGSIAPGKFHAQKVTIQDVFEAVGTHARGKMSDADLEELEHNACPGAGACGGQFTANTMSMCGEFLGISPMGANSVPAMTVEKQQVARRCGHLVMELVRRDIRPSQIITRKAIENAIASVAASGGSTNAVLHLLAIAHEMDVELNIEDFDKISSRTPLLCELKPAGRFTATDLHDAGGIPLVAQRLLEANLLHADALTVTGKTIAEALDGVAPAPDEARPLSNPIQATGHLQILKGNLAPEGAVAKITGKEGQRFQGPAKVYDSEEDMLTALEQKGIEKGDVVVIRYEGPQGGPGMPEMLTPTSAIVGAGLGQHVALMTDGRFSGGSHGFIVGHVTPEAQVGGPIGLIQTGDVITIDAEKNEISVDVSDDEMASRKAAWVAPPFKAKRGTLYKYIKNVKSASEGAVTHR",
    "response#2": "MTGQPFVGVVTTWNEAAPCNISLSRQAQVVKKGVAAAGGTPREFTTITVTDGIAMGHAGMKASLVSREVIADSVELSVRGHCYDALVGLAGCDKTLPGLMMAMLRLNVPSVFLYGGSILPGRFRGKDVTVLDVFEAVGANAAGTMSDADLAELETVACPSAGSCGGQYTANSMAYVSEAIGLALPGSASTPAPYESRDRFADASGRAVMALLKRGLRPRDIVTRQALENAAAVVAATGGSTNAALHLPAMAHEAGIAFDIFDVAAVFRRTPLIADLKPGGRYLAKDVHEIGGVPVVLKALLDGGFLHGECLTVTGRTLAEAITELTGDGPRHADVVHPTTRPLSPTGGLVGLSGNLAPEGAIVKVAGLRRLAFSGPARVFDREEDAFAAVMQRRYHAGEVLVIRYEGPRGGPGMREMLSTTSAIYGQGMGEDVALITDGRFSGATRGLCVGHVGPEAAVGGPIALIEDGDVIEIDAERGTLSLRIDPAELAARRERWQPRRHAFQSGALWRYAQTVGSAGKGLIRPLENAYSPVGGLAVLFGNLAAEGAVVKTAGIQPSMRRFTGAAICFDSQDEAIAGIMGGKVKPGHFVVIRYEGPKGGPGMQEMLSPTSLIMGMGLGESVALVTDGRFSGATRGACVGHVSPEAAEGGVIGLVQDGDRITIDVEARALTVDVPDAELARRREGFRPKRRDPGSSWLRRYAHLVTNAANGRVWPA",
    "response#3": "MKPIIGIANTWTEIGPCNFHLRQIAEAVKQGIREAGGTPMEFNTVTISDGITMGTEGMKASLVSREVIADSIELVTRGNLFDGLVCIAGCDKNLPGTVMALARLDVPGLMLYGGSIAPGHLNGKDLTVQDVFEAVGSHAAGKLDDAGLEAVEANACPGAGACGGQFTANTMAMACEFMGISPMSISGVPAFLPEKLASAKEAGKLVLELAKRDLRPSQLITKESLENAIASVAASGGSTNAVLHFLAIAHERGIALSIDDFDRISEKTPLLCDMKPGGKYVAADYQKAGGSRLLAKRLIEGGYIHSDCLNASGRTLAEGLLEALEPRDLYPVDDPKEAEGAIKILTGNLAPDGAVLKVTGDDEFYHEGPARVFENEEDAMKYVQEGHIESGDVIVIRNEGPEGGPGMREMLGVTAAVVGAGHEDDVALLTDGRFSGGTRGPMIGHIAPEAFVGGPIGALEDGDHITVDIPDRTIEVDLSDSELERRLDERDDPEPAYTNGVVAKYGSLFGSAANGVVLPIDRPLKKTGGLAILRGSLAPDGCVVKLAGHERRLHRGPARVFDTETDCYVAVRDRRIVPGDVVVIRYEGPVGGPGMQEMLSVTAALTGEGLGDSVALITDGRFSGGTHGLMIGHIAPEAALGGPIGLVEEGDEIVIDVDAGRLDLAVDGAVLDRRRAGWTAPAPHYRGGVMAKYAALVSSASQGRQVADGGKVIFPATNALKPTGGLVILKGNLAPEGCVIKVAGHSRLTHRGPARVFDREQDAFAAVENGSIQPDDVMVIRYEGPKGGPGMREMLGVTAAIAGIPALSETVALLTDGRFSGATRGLMAGHVSPEAAVRGPIAAVRNGDMIHFDIPNRRLDVELTDAQIEERLKDWKEPAPHFSRGVFKKYVDTVSSAARG"
  },
  {
    "reference": "MVIKYLLLILVQSFVAFALPFTSRSDPKAEYLVTSLPGLYSNIRTDERPLMFAGQLELYPENQTHYFFWKYQDTNQIPEAKKRTIFWLNGGPGCSSMDGALMEAGPFRINKEGEVIYNEGSWHKSGDMVFVDQPAGTGFSYSDDYDHDLDQITVEFVRFMEKFFELFPEDASNEIYFAGESYAGQYIPYIADGILRRNKNLREGEKPFNLKGLMIGNGWIAPNEQSLSYLPYSVQAGIIKTNNPRWSSILRQHQECQDIVSENDGPDGSDVSQVVSNTCERVLNLILEATRDQSAADNEQCVNMYDHTLRDSYPSCGMNWPPDLANVTPFLREQSVMNDLNLINHKKWSECSGKVGNSFRAKNSKPAIHLFPSILEEIPIMLFNGNRDIICNYIGIEGFIKKLTWNGQTGFSEDLDTLDWVYDNKTAGYIQSERNLTVVNVFDASHMVPFDKPEISRSLIDIITGNFDEKEVDNKSDMKKKSIVTYPLGVRMAKQKEESESKTSPTSVTQSKTSSISAVSGKSLATSTTLDQEHSATPSAEAERAKNQQTSNRITRLIQLLVIVVLIWGVYILYSSYRSRPSSIIKTGPSGKKKNVQWADQLRQFEEEEIEQNEQGILSRALNKLKGGDSRGTYAPTSGKTYEDIEMNEGITEHTDNRVDDFIIESDEEDAHDENQTNKQSVSK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The Cytoplasmic, Lumenal topological domain of the protein should be designed to be flexible or rigid.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. The protein should be optimized for stability and solubility when expressed in membrane, Golgi apparatus where it will be involved in apoptotic process, proteolysis.4. Ensure the presence of a signal peptide within the protein.5. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.6. Prioritize Basic and acidic residues, Polar residues compositional bias within the protein sequence to achieve desired characteristics.7. The protein must exhibit the following characteristics: Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from protein precursors. The designed protein sequence is ",
    "response#1": "SLDYANQAIDAMEHILQSRLESKSLDIVAVPGFQTGNRVSPSFIVLPEEDILYNEQSNDINQKTRIARMISNRIAAQWFGGITNPEEFGTFWLNEALPRFLEVEALEKILDINSDDLWTYEMEKILERDATATSQPLRVKNVFSSADIAEIDHEFIGKKGAAVLRMIQKSVGVNVFNKAIRSFVSSYRSAYPYDDGLWKSFEKALGGKLKGWNNEPLDVAKFVNTL",
    "response#2": "NTILYRDRRSLTAHVSFDGGEEWETVKGNDGTIEGTVDLIWRHPFDDNRAYILGENGKHWITTDQAKSWRPFQVDVLPFASMRSFPLEFNGWDPKKVIFMGLDCTILGCLPQTFYTTDDFETVKPLREMTLQCMWAASRPQFALDIDMPKSTGDRILCVVPGLKGPFEHTRTERLIYSDSFFKDDAEGTEAKLDHGKPLSGVSLQIRSVSKYIVARLQSRGTQEQALYVSDDSNVWHRAEFGKHRIEEDAYTILESTNYSIQIDVQTTWQDNRMGSLFSSNSNGTYFTQNIEHTNEDLNGLVDFERVTGIHGITIVNTVKNWEEAKKSDSNKKKLISSISFDDGRTFQPLKADKDELHLHSMTTYAALHEMPLPDHRMFSSPAPGLVMGVGNTGGHLEDYSKGDLYVSDNAGLTWRRALKGPHRYEFGDQGGVITAVLDSSQTDKVQFSIDHGKEWESISLGQKIRPLFLVSTPDSTSLKFLLVGTSSDDTTKTLMFFIDFDGLHERECGSGDLEEWTARLNEKGEPDCLMGQKQFFQRRKANADCFIKKEFAISGPEFQLCKCSAEDFECDYNFVRSEDRKECVPAVPLTPPAGKCKDPSDKYMGPSGWRLIPGNACIREGGEDLEREIERSCSNVTGPPE",
    "response#3": "NTILYRDRRSLTAHVSFDGGEEWETVKGNDGTIEGTVDLIWRHPFDDNRAYILGENGKHWITTDQAKSWRPFQVDVLPFASMRSFPLEFNGWDPKKVIFMGLDCTILGCLPQTFYTTDDFETVKPLREMTLQCMWAASRPQFALDIDMPKSTGDRILCVVPGLKGPFEHTRTERLIYSDSFFKDDAEGTEAKLDHGKPLSGVSLQIRSVSKYIVARLQSRGTQEQALYVSDDSNVWHRAEFGKHRIEEDAYTILESTNYSIQIDVQTTWQDNRMGSLFSSNSNGTYFTQNIEHTNEDLNGLVDFERVTGIHGITIVNTVKNWEEAKKSDSNKKKLISSISFDDGRTFQPLKADKDELHLHSMTTYAALHEMPLPDHRMFSSPAPGLVMGVGNTGGHLEDYSKGDLYVSDNAGLTWRRALKGPHRYEFGDQGGVITAVLDSSQTDKVQFSIDHGKEWESISLGQKIRPLFLVSTPDSTSLKFLLVGTSSDDTTKTLMFFIDFDGLHERECGSGDLEEWTARLNEKGEPDCLMGQKQFFQRRKANADCFIKKEFAISGPEFQLCKCSAEDFECDYNFVRSEDRKECVPAVPLTPPAGKCKDPSDKYMGPSGWRLIPGNACIREGGEDLEREIERSCSNVTGPPGNTILYRDRRSLTAHVSFDGGEEWETVKGNDGTIEGTVDLIWRHPFDDNRAYILGENGKHWITTDQAKSWRPFQVDVLPFASMRSFPLEFNGWDPKKVIFMGLDCTILGCLPQTFYTTDDFETVKPLREMTLQCMWAASRPQFALDIDMPKSTGDRILCVVPGLKGPFEHTRTERLIYSDSFFKDDAEGTEAKLDHGKPLSGVSLQIRSVSKYIVARLQSRGTQEQALYVSDDSNVWHRAEFGKHRIEEDAYTILESTNYSIQIDVQTTWQDNRMGSLFSSNSNGTYFTQNIEHTNEDLNGLVDFERVTGIHGITIVNTVKNWEEAKKSDSNKKKLISSISFDDGRTFQPLKADKDELHLHSMTTYAALHEMPLPDHRMFSSPAPGLVMGVGNTGGHLEDYSKGDLYVSDNAGLTWRRALKGPHRYEFGDQGGVITAVLDSSQTDKVQFSIDHGKEWESISLGQKIRPLFLVSTPDSTSLKFLLVGTSSDDTTKTLMFFIDFDGLHERECGSGDLEEWTARLNEKGEPDCLMGQKQFFQRRKANADCFIKKEFAISGPEFQLCKCSAEDFECDYNFVRSEDRKECVPAVPLTPPAGKCKDPSDKYMGPSGWRLIPGNACIREGGEDLEREIERSCSNVTGPP"
  },
  {
    "reference": "MPEIRLRHVVSCSSQDSTHCAENLLKADTYRKWRAAKAGEKTISVVLQLEKEEQIHSVDIGNDGSAFVEVLVGSSAGGAGEQDYEVLLVTSSFMSPSESRSGSNPNRVRIFGPDKLVRAAAEKRWDRVKIVCSQPYSKDSPYGLSFIRFHSAPDKEEVEASPQKVTKLGQFRVKEEDEGANSLRPGALFFSRINKTSPAAATDPAGPSYAAATLQASSATSSPSAASRAVGSTSKPQESPKRKRKLDLNQEERKTPSKPSAQPSPPAIKRPKPPAPTRTPATPVPAPARGTVPGKTREGAEPRGPRAGPQELGKILQGVVVVLSGFQNPFRSELRDKALELGAKYRPDWTPDSTHLICAFANTPKYSQVLGLGGRIVRKEWVLDCHRMRRRLPSRRYLMAGPSSSSEDEGGSHSGSSGDEAPKLPQKRPQTKTKLPQAAGPSSPQRPKTPEETKPASPGPQEDTDSEGEQSEEQGNGAEDSGDTEDELRRVAEQREQKQPPDQGENGEDPYAGSTDENTDHEGPPESPDLPVPELPDFFQGKHFFLYGEFPGDERRKLRRYVTAFNGELEDYMSDRVQFVITAQEWDPSFEEALMDNPSLVFVRPRWIYSCNEKQKLLPHQLYGVVPQA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The presence of BRCT domains is critical for the interaction of the designed protein with its intended targets.2. A protein that contributes to base-excision repair, double-strand break repair via nonhomologous end joining, hippocampus development, single strand break repair by functioning in nucleolus, site of DNA damage, chromatin, ERCC4-ERCC1 complex with oxidized DNA binding, 3' overhang single-stranded DNA endodeoxyribonuclease activity.3. The protein design should emphasize Acidic residues, Pro residues, Polar residues, Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "QPETKEEDDGLRKSLDKFYEVFGNPQPASGNSLSTSVCQCLSQKINELKDQENQTYTLRSFQMARVIFNQNGCSILQKHSRDAHFYPVREGSTSLQDEKLTPGLSKDIIHFLLQQNLML",
    "response#2": "RRSELITITTGSKQLDTLLQGGVETGSITELFGEFRTGKSQICHTLAVTCQLPIDMGGGEGKCLYIDTEGTFRPVRLLAVADRYGLNGEEVLDNVAYARAYNADHQLELLQQAANMMSESRFSLLVVDSCTALYRTDFSGRGELSARQMHLARFMRTLQRLADEFGIAVVITNQVVAQVDGISFNPDPKKPIGGNILAHSSTTRLSLRKGRGEQRICKIYDSPCLPESEAIFAINSDGVGDELPDFFQGKHFFLYGEFPGDERRKLIRYVTAFNGELEDYMSDRVQFVITAQEWDPSFEEALMDNPSLAFVRPRWIYSCNEKQKLLPHQLYGL",
    "response#3": "QPETKEEDDGLRKSLDKFYEVFGNPQPASGNSLSTSVCQCLSQKINELKDQENQTYTLRSFQMARVIFNQNGCSILQKHSRDAHFYPVREGSTSLQDEKLTPGLSKDIIHFLLQQNLMA"
  },
  {
    "reference": "MVDFYYLPGSSPCRSVIMTAKAVGVELNKKLLNLQAGEHLKPEFLKINPQHTIPTLVDNGFALWESRAIQVYLVEKYGKTDSLYPKCPKKRAVINQRLYFDMGTLYQSFANYYYPQVFAKAPADPEAFKKIEAAFEFLNTFLEGQDYAAGDSLTVADIALVATVSTFEVAKFEISKYANVNRWYENAKKVTPGWEENWAGCLEFKKYFE",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The presence of GST N-terminal, GST C-terminal domains is critical for the interaction of the designed protein with its intended targets.2. The designed protein should have a high specificity for glutathione binding, with minimal off-target effects.3. The protein must exhibit the following characteristics: Has DDT dehydrochlorinase activity; May be involved in detoxification.; Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.4. The protein should have a fast turnover rate for 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane = 1,1-dichloro-   2,2-bis(4-chlorophenyl)ethylene + chloride + H(+).5. The target protein must exhibit strand-helix-strand-helix-helix-helix-strand-strand-helix-strand-helix-helix-helix-turn-strand as its primary conformation.6. The designed protein should have glutathione peroxidase activity, glutathione transferase activity, DDT-dehydrochlorinase activity within cytoplasm to regulate glutathione metabolic process. The designed protein sequence is ",
    "response#1": "MTFATVYIKPHTPRGDWLASLGQYVGLEIKTVDYKSAEASKFEELFPLKRVPALVTPNGFQLTELIAIVEYIVAKGSKPYCAWEEAQVNSVADQFKDYLNEVRPYFMVKMGFAEGDLDALAKDVFLPGFKKHYGFFANFLKSAGSGYLVGDSLTFVDLLVAQHTADLLAANAALLDEFPQFKAHQEKVHSNANIKKWLETRPVTPAEAEGWE",
    "response#2": "MPAKLGYWKIRGLQQPVRLLLEYGEKYEEQIYERDDGEKWFSKKFELGLDLPNLPYYIDDKCKLTQSLAILRYIADKHGMIECPFTHRAILARSLKKLEPVLGLVLSHWQLDSKGARFLPAPHRPEKYKERFFTATGGIASAKLDESEELGDVNNDSARLFVDGAFDPVENISRLSELYYLNDPKYPGTKFTVPVLWDSKTRKIVNNESGDIIRILDSLPRHFSCPFTHRAILARSLKKLEPVLGLVLSHWQLDSKGARFLPAPHRPEKYKERFFTATGGIASAKLDESEELGDVNNDSARLFVDGAFDPVENISRLSELYYLNDPKYPGTKFTVPVLWDSKTRKIVNNESGDIIRILDSLPRHFSGN",
    "response#3": "MTFATVYIKPHTPRGDWLASLGQYVGLEIKTVDYKSAEASKFEELFPLKRVPALVTPNGFQLTELIAIVEYIVAKGSKPNTCPWAQRTLITRALKGLAPIIGCSVAHWHLDDKGWRFLEEGDGKTNERHWFDIAGGISSVNLNTSTPVANIPNNAHRLLVDGTDEPHYGYKRLSDFYFKTKPDYKGRFTVPVLWDLETCTIVNNESSDIIGINDTEAAEVDAIADQFKDYLNDVSPYLTVLAGFKPGDKDQLRTDVFVPAFKKNFEFFENILASNHSGFFVGNSLTWVDLLISQHVQDILDKDLAVVEEFKKVLAHRKKVQSIDRIQKYIANRPDYPL"
  },
  {
    "reference": "MDSLSRPPIAAIILAAGKGTRMKSNLHKVLHPIAGRPMLLHLAASVAALAPVRTVVVTGAGREQVEAAVSPLGIATALQAEQLGTGHAVAQAEAALSGFVGDVLILYGDVPLVSSQTMQAMLDRLNAEDAPVAVVLGFRPADAGAYGRIIAEDGVIAKMVEYKDATPAERAVDLCNSGLMAVRSADLFALLAHVGNDNAAGEYYLPDIVMLAAADGRRSAVIETGATEVAGVNSRGELAVVEASWQAQRRAQAMTDGATLIAPETVWFAHDTAIGRDVVIEPNVVFGPGVTVGDGAVLHAFSHIEGAKIGAKAEVGPFARLRPGAVMGEKSKIGNFVEMKKAVLGAGAKASHLTYLGDAEIGAGANIGAGTITCNYDGFFKAKTIIGEGAFIGSNSALVAPVTIGAGAIVGAGSVITADVEADALALGRGKQEARAGWAKRFRDAMKAKKAKA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must possess magnesium ion binding to support lipid A biosynthetic process under certain conditions.2. A protein that can modulate the overall flux of metabolites through the Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway, enhancing or suppressing its activity as needed.3. The designed protein should have a high specificity for acetyl-CoA binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "GVTVLDPATTWIDVDVTLAPDVTLLPGTQVLRATTVGEGATIGPDTSLVGCEVGAGATVTRTDATFAIIGERATVGPFAYLRPGAELGRSGKIGTFVEVKNSTIGSGSKVPHLSYIGDTEIGDRVNLGAGAITANYDDLAKHRTVIGDDVHSGSHNVFVAPVRIGDGAKTGAGAVIRKDVPSGALALSVAPV",
    "response#2": "GVTVLDPATTWIDVDVTLAPDVTLLPGTQVLRATTVGEGATIGPDTSLVGCEVGAGATVTRTDATFAIIGERATVGPFAYLRPGAELGRSGKIGTFVEVKNSTIGSGSKVPHLSYIGDTEIGDRVNLGAGAITANYDDLAKHRTVIGDDVHSGSHNVFVAPVRIGDGAKTGAGAVIRKDVPSGALALSVAPR",
    "response#3": "VIMAAGKGTRMKSRHPKVLQKLAGRALLQHVLDTAAQLKARSAVVITGHGAAEVEAALSGAQNAEAGFELKFARQEPQLGTGHAVQQAVPALAGDGMVVVLSGDVPLTQADTLQALVDAAGGDKLRR"
  },
  {
    "reference": "MDSSKIRIRLKSYDYRMLDISAAEIVETARRTGARVCGPIPLPTKIERFTVLRSPHVDKNARDQFEQRTHKRLLDILDPNDKTVDALIKLDLAAGVDVEIKL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Involved in the binding of tRNA to the ribosomes..2. A protein that contributes to translation by functioning in ribosome, ribonucleoprotein complex with structural constituent of ribosome, tRNA binding. The designed protein sequence is ",
    "response#1": "MAPGACMPLGKVPLGTTVHNVELKPGAGGQLVRSAGSSARVIARDGNYTLVTLPSGEKRLILSTCHATIGAVSNADRKNTKLGKAGRSRWLGRRPSVRGVAMNPVDHPMGGGEGKTSGGRHPVSPWGFK",
    "response#2": "MAPGACMPLGKVPLGTTVHNVELKPGAGGQLVRSAGSSARVIARDGNYTLVTLPSGEKRLILSTCHATIGAVSNADRKNTKLGKAGRSRWLGRRPSVRGVAMNPVDHPMGGGEGKTSGGRHPVSPWGFK",
    "response#3": "MAPGACMPLGKVPLGTTVHNVELKPGAGGQLVRSAGSSARVIARDGNYTLVTLPSGEKRLILSTCHATIGAVSNADRKNTKLGKAGRSRWLGRRPSVRGVAMNPVDHPMGGGEGKTSGGRHPVSPWGFK"
  },
  {
    "reference": "MEPVDPNLEPWKHPGSQPTTACNRCYCKKCCWHCQLCFLKKGLGISYGRKKRRHRHGTPQSRQDHQNLVSKQPLPISRRNPTGQKESKKEVASKTETDPCA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The ideal protein would be one that plays a critical role in modulation by virus of host chromatin organization through protein serine/threonine phosphatase inhibitor activity within host cell cytoplasm, extracellular region.2. The protein must exhibit the following characteristics: Tat can reactivate a latently infected cell by penetrating in it and transactivating its LTR promoter; This also contributes to the increase in proviral transcription rate, especially when the provirus integrates in transcriptionally silent region of the host genome. The designed protein sequence is ",
    "response#1": "MXXXFXKMX",
    "response#2": "MSPQTETKAGVGFQAGVKDYKLTAVL",
    "response#3": "MQLAR"
  },
  {
    "reference": "MSNVPNELKYRDSHEWVRKEADGSYSVGITEHAQELLGDMVFVDLPEVGNHYRQGDDCAVAESVKAASDIYAPLSGEIIEVNSALASEPERVNSAPYGDGWLFRLKASDEGELATLLDATAYQTAIAE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that The H protein shuttles the methylamine group of glycine from the P protein to the T protein. The designed protein sequence is ",
    "response#1": "TKANTPATRAGSGNAAAAKADTVALLDDTTLAVPGDKPSALFFFSVGCGECAGGAKSLDKAAQAFDKAGKKANFLAVDMDPNESKQTIMQFLDYIKAPALPATIDKGAALSQRYQVAALSTLIVVAPQGKVTYRATDPSADQIQDALKKAGF",
    "response#2": "MLTYKTFKILGREILIGVVWEEKIQGIAYSLDGLEFLKDQLSRVTSHLKSRGVKVNLLEEKSRYPDLVFDVLKGKIGNEKGFEEN",
    "response#3": "MLTYKTFKILGREILIGVVWEEKIQGIAYSLDGLEFLKDQLSRVTSHLKSRGVKVNLLEEKSRYPDLVFDVLKGKIGNEKGFEEA"
  },
  {
    "reference": "MISHRNAIANIMQIVTYESTYQSDEPELCLGVLPQSHIYSLVVVSQASIWRGDGVVVLQGFELEQTLLAIQTNGIKRLWLVPPMLVAITKAPRIVESYDLSSVSVAAVGASGISKDVMATFGELLPACKIIQGYGMTETTGVVCFGNVEDSMDGSCGHLYPGYEARLIDGEGKDVESHNTPGELVLRSPSVVIGYYNDESATSEAMMDGGWLRTGDLVEIRQSEKGHEHVFVVDRVKELIKVRGLQVAPAELESHLILHPAVAEVAVIPVPDDRAGELPKAYIVRASGAELDEQVLRKELSQYVEGQFARHKHLDGGIEFLDSLPKTASGKMQRKTLKEKARTDAEARRQAREKAANGVHKVHVNGVKRPEKMEVFDLSSDDEDDD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that The linear polyketide carboxylic acid, which is released from PKS, is converted to a CoA thioester by lcsD, and then lcsE hydrolyzes the thiol bond and shuttles the polyketide intermediate to lcsA (Probable); Further hydroxylation steps performed alternatively by the cytochrome P450 monooxygenases lcsI, lcsK and lcsN then yield the non-methylated leucinostatins precursor The designed protein sequence is ",
    "response#1": "MISHRNAIANIMQIVTYESTYQSDEPELCLGVLPQSHIYSLVVVSQASIWRGDGVVVLQGFELEQTLLAIQTNGIKRLWLVPPMLVAITKAPRIVESYDLSSVSVAAVGASGISKDVMATFGELLPACKIIQGYGMTETTGVVCFGNVEDSMDGSCGHLYPGYEARLIDGEGKDVESHNTPGELVLRSPSVVIGYR",
    "response#2": "MISHRNAIANIMQIVTYESTYQSDEPELCLGVLPQSHIYSLVVVSQASIWRGDGVVVLQGFELEQTLLAIQTNGIKRLWLVPPMLVAITKAPRIVESYDLSSVSVAAVGASGISKDVMATFGELLPACKIIQGYGMTETTGVVCFGNVEDSMDGSCGHLYPGYEARLIDGEGKDVESHNTPGELVLRSPSVVIGYG",
    "response#3": "FRRQVLSRPDEEAVHAWDMSATYAELDDMSSLLAHHLVSQEGHSAGESLIPFCMEKSGLALVVMLAIMKAGCGYVPLDPSHPIDRRAQIVSDTGTSIVFVTPETLRDFSGAATSEISERDVKFIVVSHDFIKKLQRGSPAALPSTDPSSTAYTLFTSGSTGKPKGVVMSHRAACSAITAQARAFLMTPSTRAINFAAFVFDASVLEIFGTWIAGGCVAVPSDSIRLNGLVARFMTESQTDWALFTPTFATTLTPKDVPTLRVLVLGGEAIRKENVETWLSKVELFNAYGPTEACVATVHHRIMSPTDTGVIGKSVGCRVWVTKVDEINTLAPVGCAGELVIEGPGLATGYATAIASALANFLIKEEGSIKVEDSPEHAGLDSLVAMELRNWIRQRFGVDTTVMTIVQSTSIMSLGDYIRTAG"
  },
  {
    "reference": "LGDQKIPFLGPSDRGFQQLWDSSYTGLVLRRSCSLPAELHGQVQAALLTLRSKGCLLRDLVRVRDRDVFTAVSRALLGEPGHTYRYLDTRLFAIPWHSEDTEVKGQNCCDPDLRSACKLKHSLSDYHIEDKEEKASGQGCSQPSPPQVCTGPVKFNVTLLNYMDPAAMSQLKEEPYYGMGKMAVGWHHDENLVTHSPVAVYSYSCESSEGGSGEKACWRIGLKVAWDIHTPGLMLPLESGDCYYMRDDLNSTHQHCVLAGDTARFSSTHRVAECSSGTLTYIQSRCQEALSNLHTDPETGSHSLLALLPTTLQHCEEIHNEVEFEWLRQYWFQGQRYTRFCSWWTRPMEQLEKDWKLMETMTMLFLATVEEEGRAGEGRREMAETLLSALTDRHQQRQTWRDRCHSSLAQTLPPEEAPVDRPFWGVDDPSMPLPFDLADIINRVESLLWRCAQDVEVYKCAYVHIYSP",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have metal ion binding, oxidative RNA demethylase activity, oxidative DNA demethylase activity within cytoplasm, nuclear speck to regulate regulation of multicellular organism growth, DNA dealkylation involved in DNA repair, positive regulation of canonical Wnt signaling pathway, oxidative single-stranded RNA demethylation.2. The protein contains novel Alpha-ketoglutarate-dependent dioxygenase FTO catalytic domains that confer a unique function or activity.3. The protein have enhanced 2-oxoglutarate + an N(6)-methyladenosine in mRNA + O2 = an   adenosine in mRNA + CO2 + formaldehyde + succinate. The designed protein sequence is ",
    "response#1": "GTLDYILQRCRLAVQNVCDDVDNGDVSLKSFEPAVLKQGEEIHNEVEFEWLRQFWFQGCRYRKCTDWWCEPMTQLEGLWRKMERVTNAVLQKVKREGLPVEQRNEMLTAILPLLTARQNLRREWQARCQSRIARSLPGAQKPQCRPYWEDDPSMPLPFDLTDIISELL",
    "response#2": "MEDYEIKYCHPELFAACKALIKLNDYLNVEAVQALQEQNLSETKETENTAVTDREQDFSTFIKGSLLEVQSSLYTPRNDCTSLKYRTSYNLTLLNYMDPLQMPYLKQEPYFGMGNMAVSWHHDENLIERSTVAVYNYSCEGSACDKVNEQYVEGRDPADWHLGLKCSTGTLEYIFGQHPMFDEILARSFVGFVHENAAKLPKDLHAGMRLAFEKLRDKGYFQYDVVMAGGKHLSRTFVRRTLVGDFGITYKYLGLRIFAHAWNRPSSAPVFQSLYDLNQQMIARTKRSMVPAPPLCEYNLTLINYMEPDNPLLGLKDDPTYGMGKVSVSWHADSSLQDYSSIGVYHTLLPTKQKQPKTRCDWRIALRLSPEVPGADQTPPLVLPTDDGDAYYLNGDFNHFHQHMVLSGTAVRVSSTHRVAVDTLQYIQGRVARALKSSANGNSPFRDGEVDAVREEQATLTVVEMDWIRQYWIQGVKHDELHTDWQGPMRKLEASWLVLEERTKTLVDALLKTQDIEHLKVLKAVLEGLRARQAGRDQFVHRMRDKVFKRIHADFQPTERPTWKSGILPKDLTDVIAKLL",
    "response#3": "DDGFHQLWKTKYSKLILKNAEMIPKEVHKGVQEAFLTLRKHACFFQDLVRIKGKDFFTPVSRILIGNPGCTYKYLNTRLFTVPWPVEGYEINYSHPEISKACKALIKLNDYLHGETVLALREQSLAETKEREDPPVTDSHRDYVPPITGTGPFLQDQSPSRVPKNDCANLKGRTSYNLTLLNYMDPQQIPFLKQEPYYGMGKMAVSWHHDENLVERSTVAVYSYCCEESTVDKLETEASNGRDPAIWHVALKVAWDIKTPGLAIPLHQGDCYFMLGNIQIGKHICFLQEFCVRRVLSDTFQYIKRRCLEALALIPDLENKSASLDAKKIQLLAEIHREVEFQWIRMFHLQGDTHARQHKTYWTQRIAELTEAWDRMEACFRAILSKLKLSPESTPQPSRAYSMMLYAMKEVKELRDEHVKRISASAYAALPESQRPVDLPQYDDTSPLPFELKPVIYFKID"
  },
  {
    "reference": "MRDSTGAGNSLVHKRSPLRRNQKTPTSLTKLSLQDGHKAKKPACKFEEGQDVLARWSDGLFYLGTIKKINILKQSCFIIFEDSSKSWVLWKDIQTGATGSGEMVCTICQEEYSEAPNEMVICDKCGQGYHQLCHTPHIDSSVIDSDEKWLCRQCVFATTTKRGGALKKGPNAKALQVMKQTLPYSVADLEWDAGHKTNVQQCYCYCGGPGDWYLKMLQCCKCKQWFHEACVQCLQKPMLFGDRFYTFICSVCSSGPEYLKRLPLQWVDIAHLCLYNLSVIHKKKYFDSELELMTYINENWDRLHPGELADTPKSERYEHVLEALNDYKTMFMSGKEIKKKKHLFGLRIRVPPVPPNVAFKAEKEPEGTSHEFKIKGRKASKPISDSREVSNGIEKKGKKKSVGRPPGPYTRKMIQKTAEPLLDKESISENPTLDLPCSIGRTEGTAHSSNTSDVDFTGASSAKETTSSSISRHYGLSDSRKRTRTGRSWPAAIPHLRRRRGRLPRRALQTQNSEIVKDDEGKEDYQFDELNTEILNNLADQELQLNHLKNSITSYFGAAGRIACGEKYRVLARRVTLDGKVQYLVEWEGATAS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should contain one or more Tudor domains that are essential for its function.2. For general function, the protein need meet that Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex ; Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation ; Polycomb group (PcG) protein that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex, thus enhancing PRC2 H3K27me3 methylation activity; Binds to the metal- regulating-element (MRE) of MT1A gene promoter .3. The designed protein's functionality will benefit from Polar residues, Basic and acidic residues compositional bias in its structure.4. The ideal protein would be one that plays a critical role in negative regulation of transcription by RNA polymerase II, positive regulation of histone H3-K27 methylation, stem cell differentiation through metal ion binding, chromatin binding within nucleoplasm, focal adhesion, cytoplasm, ESC/E(Z) complex.5. The target protein must exhibit strand-strand-turn-strand-strand-helix-strand-turn-strand-turn-strand-turn-strand-turn-strand as its primary conformation. The designed protein sequence is ",
    "response#1": "MQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYGNGYEKFLGVLRAARDLALDTLIIEYRGKVMLRQQFEVNGHFFKKPYPFVLFYSKFNGVEMCVDARTFGNDARFIRRSCTPNAEVRHMIADGMIHLCIYAVSAITKDAEVTIQRGLKKHLLLAPSDVAGWGTFIKESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERPGSVEVRYVDSIKGKGLFATQLIRKGETIFIERPLVAAQFLWNALYQYRACDHCLRALEKAEENAQRLTGKPSQILPHPELCSVRKDLHQNCPHCQVMYCSAECRLAAAEQYHQILCPGPSHDPRHPLNKLQEAWRSVHYPPETASIMLMARMVATVKQAKDKDHWVRLFNHFCSRTANQEQAIVHKLLKGKFKDQLELLLGLFKEALYEEALSLWFTPEGFRSLFALVGTNGQGIGTSSLSQWVHACDALELTPQDREQLDTFIDQLYKDIEAATGEFLNCEGSGLFVLQSCCNHSCVPNAETSFPENNFVLHVTALEDIKPGEEICISYEGLCLMQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWY",
    "response#2": "GSQMQLQLGRVTRVQKHRKILRAARDLALDTLIIEYRGKVMLRQQFEVNGHFFKKPYPFVLFYSKFNGVEMCVDARTFGNDARFIRRSCTPNAEVRHMIADGMIHLCIYAVSAITKDAEVTIAFDYEYSNCNYKVDCACHKGNRNCPIQVRLQVFKSEKKGWGVRCLDDIDKGTFVCIYSGRLLRRATPEKTNIGENGREQQHIVKNSFSKKRKLEVVCSDCDAHCDSPKAEDCPPKLSGDLKEPAVEMNHRNISRTQHHSVIRRTKSKTTVFHYSEKNMGFVCSDSAAPEDKNGFKPAQEHVNSEARRAHEDLSSNPAGDSEDTQLTESDVIDITASREDSAPAYRCKHATIVDRKDTKQVLEVPGKKSQEEEPAASQSQQALCDEELPSERTKIPSASLMQLSKESLFLLDASKEGNVGRFLNHSCCPNLWVQNVFVETHDRNFPLVAFFTNRYVKARTELTWDYGYEAGALRAARDLALDTLIIEYRGKVMLRQQFEVNGHFFKKPYPFVLFYSKFNGVEMCVDARTFGNDARFIRRSCTPNAEVRHMIADGMIHLCIYAVSAITKDAEVTIGWGVRCLDDIDKGTFVCIYSGRLLRRATPEKTNIGENGREQQHIVKNSFSKKRKLEVVCSDCDAHCDSPKAEDCPPKLSGDLKEPAVEMNHRNISRTQHHSVIRRTKSKTTVFHYSEKNMGFVCSDSAAPEDKNGFKPAQEHVNSEARRAHEDLSSNPAGDSEDTQLTESDVIDITASREDSAPAYRCKHATIVDRKDTKQVLEVPGKKSQEEEPAASQSQQALCDEELPSERTKIPSASLMQLSKESLFLLDASKEGNVGRFLNHSCCPNLWVQNVFVETHDRNFPLVAFFTNRYVKARTELTWDY",
    "response#3": "MQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYGNGYEKFLGVEGLCLMQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYQRGLKKHLLLAPSDVAGWGTFIKESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERPKHLLLAPSDVAGWGTFIKESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADEGLCLMQTSFGDVPHFGVFCSDFIAKGVRFGPFRGRVVNASEVKAHRDNSRMWEIFEDGHLSHFIDGKGSGNWMSYVNCARFPKEQNLLAVQHQGQIFYESCRDIQRNQELLVWYSNTQRGLKKHLLLAPSDVAGWGTFIKESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERP"
  },
  {
    "reference": "MSARIGAISESATLAVDAKAKALKAAGRPVIGFGAGEPDFPTPDYIVEAAVEACRNPKYHRYTPAGGLPELKAAIAAKTLRDSGYEVEASQVLVTNGGKQAIYEAFAAILDPGDEVIVPAPYWTTYPESIRLAGGVPVDVVADETTGYRVSVEQLEAARTERTKVVLFVSPSNPTGSVYSEADAKAIGEWAAEHGLWVLTDEIYEHLVYGEAKFTSLPVLVPALRDKCIIVNGVAKTYAMTGWRVGWVIAPQDVIKAATNLQSHATSNVSNVAQVAALAAVSGNLDAVAEMRKAFDRRRQTMVKMLNEIDGVFCPTPEGAFYAYPSVKELLGKEIRGKRPQSSVELAALILDEVEVAVVPGEAFGTPGYLRLSYALGDEDLVEGVSRIQKLLAEAKA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a high specificity for L-aspartate binding, with minimal off-target effects.2. The designed protein must be able to regulate cellular biosynthetic process. The designed protein sequence is ",
    "response#1": "FSIDRNLLGRSIEAGPLEDPMTEPPEEIYLMTKAIADTPNEPEYVDIGFNQGIPVSLNGENLAPVTLISQLNDLVGKHGVGRLDMIENRVVGIKSREIYEAPALLVLIDAHRDLESLTLTGDVTQYKRGIEDTYGQLIYKGLWYSPLKEAIDAFILKTQEQVTGSVRVKLFKGNAKVVGRQSVNSIYSPDLATYGAEDQFDHKAAEGFIYVWGLPSKVL",
    "response#2": "FSIDRNLLGRSIEAGPLEDPMTEPPEEIYLMTKAIADTPNEPEYVDIGFNQGIPVSLNGENLAPVTLISQLNDLVGKHGVGRLDMIENRVVGIKSREIYEAPALLVLIDAHRDLESLTLTGDVTQYKRGIEDTYGQLIYKGLWYSPLKEAIDAFILKTQEQVTGSVRVKLFKGNAKVVGRQSVNSIYSPDLATYGAEDQFDHKAAEGFIYVWGLPSKVL",
    "response#3": "FSIDRNLLGRSIEAGPLEDPMTEPPEEIYLMTKAIADTPNEPEYVDIGFNQGIPVSLNGENLAPVTLISQLNDLVGKHGVGRLDMIENRVVGIKSREIYEAPALLVLIDAHRDLESLTLTGDVTQYKRGIEDTYGQLIYKGLWYSPLKEAIDAFILKTQEQVTGSVRVKLFKGNAKVVGRQSVNSIYSPDLATYGAEDQFDHKAAEGFIYVWGLPSKVL"
  },
  {
    "reference": "MVLTLCFHSDRDHHFLSTLPLAEIRRLAPGGFSVFVPEDLLPHLSPSPSCPTPPLPRELTWSAACNPTNFPHLFRHHRPPPPCTRLTPRPPPPTPAPPSASTLPRVFHGGAPPAAFQPAIDFLHNTIQKDTIASSIIAALNPSLTSSLTLYPYALPPRWPSALNQAGIPATSYGHQSHPHPIHKTIETHLLHEHWANRATLPSTVMFMKRSKFDKLRVSNAALVKSASNFLHLLNPILTARDADRYTHLPLPDTLPSTPLYFMHHSLMYFSPSQIAGLFLAAPFLERLYASLVLPAESTIGSHPFFPSLYRYRTTGEHLHYVLEGNPSSSYTQPLTATQWLTTSSITAGDLHLTVTVLESWFSVHSILITRGVRPLELPRDIISLPSPDAVLLPNPSAFDIPLRSRLVPRDVCESLFVYVRAVRTLRTTDPAGFIRTQSNKAEFDWVTAEAWDHLAQFALLTAPVRPNTYFLPLLSPLAVVRHWLFRKQRPIFATLTLLSASTAAAIPIAIARLRTHSVTQLTILGHHFTPPKILARLPVALKRLIPKRLLPHLPSHLRPPPSWSPVFTLTFSELPKARFLTFPISGQTHTLLRQLHVPAILFAPQRPSRPLIFAGLLIGTVPVLYGAYRWFVSRFDPQTVYNRYSDLLHRPTWHLTFEREPLSCFPTPFLPHPSSHPRRARRLPPLPPAPPLPPQPPPPPPPQPSPHPPLFPASIPSPPPRPSSPPPPATSPASTPALTPIPAPKTAPPLTFPSPTLVAEPDAPVTA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The ATP binding site should be stable and able to withstand changes in environmental conditions.2. A protein with mRNA methyltransferase activity, cysteine-type endopeptidase activity, RNA-dependent RNA polymerase activity functions is required to enable RNA processing, viral RNA genome replication.3. The protein should exhibit Pro residues compositional bias.4. The designed protein must have at least one (+)RNA virus helicase ATP-binding, RdRp catalytic domain.5. The protein must exhibit the following characteristics: The central part of this protein possibly functions as an ATP-binding helicase and/or methyltransferase (Probable).; RNA replication protein replicates the viral genomic RNA.6. The For protease activity active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "VTGGSAGHSTTGLVHLFAPGPSQNLHLVNSNGSWHINRTALNCNDSLNTGFIAGLFYHRRFNSSGCPERLASCRSLDVFSQGWGRLSAYNTSGPSDDKPYCWHYAPRPCGIVPGSTVCGPVYCFTPSPVVVGTTDRQGVPTYTWGENETDVFILNSTRPPGGAWFGCTWMNGTGFVKTCGAPACLVTTSNRTWNCPTDCFRKHPETTYTRCGAGPWLTPRCMVDYPYRLWHYPCTVNFTVFKVRMYVGGIEHRLNAACNWTRGEPCDLHHRDRTELSPLLLSTTQWQVLPCSFTTLPALSTGLIHLHQNIVDVQYIYGIGSSVVSWALKWEYIVLAFLLLADVHPTREISIPGQVKNLVELCQIDTLIPINSVTGNTNNTSIYTVTLTRETSLAQEIFAIKVDIASDPLATTLIGEIASYYTHWTGSIRFSFLFCGSANTTLKLLIAYTPPGIRKPRNRKEAMLGTHVVWDVGLQSTISMVVPWVSASHFRNTTPDTYSLAGYVTCWHPTKEIFIPGQVKNLIEMCQVDTLIPVNNVQASKNNISMYTVQLTTQTDVAKEVFAIPVDVASQPLATTMLGELSSYFTHWTGSLRFSFMFCGTANTTLKLLIAYTPPGVPKPDTRKKAMLGTHLVWDVGLQSTVSMIVPWVSASHFRFTTPDTYSKAGYITCWHPTREISIPGQVKNLVELCQIDTLIPINSVTGNTNNTSIYTVTLTRETSLAQEIFAIKVDIASDPLATTLIGEIASYYTHWTGSIRFSFLFCGSANTTLKLLIAYTPPGIRKPRNRKEAMLGTHVVWDVGLQSTISMVVPWVSASHFRNTTPDTYSLAGYVTCW",
    "response#2": "MMELAEIDTMIPFDLSATKKNTMEMYRVQLSDKPHTDDPILCLSLSPASDPRLSHTMLGEILNYYTHWAGSLKFTFLFCGSMMATGKLLVSYAPPGADPPKKRKEAMLGTHVIWDIGLQSSCTMVVPWISNTTYRQTIDDSFTEGGYISVFYQTRIVVPLSTPREMDILGFVSACNDFSVRLLRDTPDTSSNRFYTLDSKMWNSTSKGWWWKLPDALKDMGIFGENMFYHFLGRSGYTVHVQCNASKFHQGTLLVVMIPEHQLATVNKGNVNAGYKYTHPGEAGREVGTQVENEKQPSDDNWLNFDGTLLGNLLIFPHQFINLRSNNSATLIVPYVNAVPMDSMVRHNNWSLVIIPVCQLQSNNISNIVPITVSISPMCAEFSGARRXYDVTPPIDIPGEVKNMMELAEIDTMIPFDLSATKKNTMEMYRVQLSDKPHTDDPILCLSLSPASDPRLSHTMLGEILNYYTHWAGSLKFTFLFCGSMMATGKLLVSYAPPGADPPKKRKEAMLGTHVIWDIGLQSSCTMVVPWISNTTYRQTIDDSFTEGGRXGXLATFLTTDLSKYCLNWRYQTIKPFARAINQLMGLEHFFEWIHLRLMNTTMYVGDPYNPPQEVVTGDINDQPNDDIFIVSARGGIEGLCQKLWSMISISAINLAATRADCRVACMVQGDNQVIASTKEIHCSDDPEQGXA",
    "response#3": "MMELAEIDTMIPFDLSATKKNTMEMYRVQLSDKPHTDDPILCLSLSPASDPRLSHTMLGEILNYYTHWAGSLKFTFLFCGSMMATGKLLVSYAPPGADPPKKRKEAMLGTHVIWDIGLQSSCTMVVPWISNTTYRQTIDDSFTEGGYISVFYQTRIVVPLSTPREMDILGFVSACNDFSVRLLRDTEDMVETRYVQTSQTLDEMSMESFLGRSGCIHISKLVVEYEGYDDTKNFKTWKINLQEMAQIRRKFEMFTYTRFDSEITLVPSIAAKGDDIGHVVMQXXXXXXXXXXXXXXXXXXXXXXXXXXSIFWQYGQTYPRFSLPFLSIASAYYMFYHPTKEIFIPGQVRNLSELCQVDTIVPINHTTSTTSSVNMYTIQLTKEQPGTEVFAMRVDVASQPLATTLIGEISSYYTNWTGSLRFSLMFCGSAGTTLKMLVAYTPPGIAKPETRKDAMLGTHVVWDVGLQSTMSMIVPWISASXXXXTTPDTFSQAGYITCWPPNPYANQTHTHAACKVIENRMLEIVGMHLPKHSCTMLFLKRSKLRYMRRAAILKDVFLNKDVEPKDLFRYDRDTIRSRLQDIDTKIAYMSDTLHFMSRREIVQLFEDSPKLETLLATVVLPVEALHKRTSLYPSLYSINYSAKGFEYIPGNHGGGSYFHPYTTLEWPPAPPAPEPEEGDTSQDIANAVVAYGVWPHYLSAEDANAIDKPTRPDTSSNRFYTLESKQWMENSKGWWWKLPDALKDMGIFGENMFYHFLGRAGYTIHVQCNASKFHQGTLMVVAIPEHQLAYIGTGNVTVGYKHTHPGESGRIIANNADNNTRQPSDDNWLNCDGTLMGNLPIFPHQFINLRTNNSATLILPYMNCVSMDSMLRHNNWSLLIIPVCPL"
  },
  {
    "reference": "MNNLRETYIKDVKRIVVKVGTSTLTYPTGLLNLNKIENLVRQLSDAQNRGIEVILVSSGAIGAGIGKLGLKERPKTIPEKQAAAAVGQGILLHMYEKIFSEYGKPVGQILLTREDLSHRTRFLNASNTFYSLLEQGVIPIVNENDAIVVDEIKFGDNDTLSAMVASLVDADLLILVTDIDGLYDSNPKTNPDAKFIPVVDEITEDIVAAAGGAGSSLGTGGMATKINAGKIATSSGSSMVIVNGDNRNFLTDILDGKEIGTLFLGQEDHVKAKKHWMAFATKPTGSIIVDDGAEKALVNHNKSLLPKGIISIDGTFQEGEVVSILNSKKEEIAHGITNYNSMEIDLIKGLDSNIIEDKLGYKNYDEVIHKNNLVILENL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The ATP, substrate binding site should be located in a region of the protein that is accessible to the ligand.2. The protein should be designed to have high stability and activity towards ATP + L-glutamate = ADP + L-glutamyl 5-phosphate.3. The designed protein must have at least one PUA domain.4. The designed protein should be involved in phosphorylation, L-proline biosynthetic process by localizing to cytoplasm and exhibiting RNA binding, glutamate 5-kinase activity.5. Ideal protein should have a binding affinity for key enzymes in the Amino-acid biosynthesis, L-proline biosynthesis pathway to enhance the overall efficiency of the pathway.6. The protein must exhibit the following characteristics: Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.. The designed protein sequence is ",
    "response#1": "MRIVVKLGTSVLTDGTDRLHRPRMVDLARQMAQLREAGHEVVLVSSGAVLAGWERLGFPKRRRELTHKQALAAVGQGRLMHIYGQLFEIYDVPVAQTLLTRADLRDRVRYLNARATLLTCLEIGVLPIINENDAVAVDEIRVGDNDTLSALVANLVDAQLLVILSDIAGLYSADPRHNPEATLIDDVAAVNEQVYALAGAAGSHRGTGGMFTKIQAAELATRAGTTMVIRLAGSEENIRLTGKVGSSLLAADGGGLSPRFALGLAQFVSANLAAGREVVIVSSGAVAAGRAILPKAAEAGAAIAARQALAALGQAQLIALWQRFFERPVAQVLLTHDDLRNRRRYLNARATLGELLRLGALPVINENDTVSVDELKLGDNDNLAAIVAALVDADALFIATDIDGLYSADPRSNPLARPLDEVAELSAEVLAMAGGSGSSVGTGGMRTKLEAAAKAGAAGIETYLFNGRSAEVVRGLAQDRLCGTRILYPGEPQKLMGGRLLLDDGAALAIRERGTSLLPVGVSAVEGDFLRGEAVACVDAAGHEVARGLVNYSSDEARQIMRKSTREIEGTILIDAGAALALTDKGASLLPGGVAGAEGDFRRGDMVEIHLRDSVGSRCLARGVSQYSALDVRRIARRHSREIEPILFSGRLILDAGAVNALHAGKSLLPIGVIAAEGEFERGAAVACISPDGREIARGLSNYGSGEARLIARKTTQEIENTLGYVDEPEIAHIAVDAGAVQALTQQGRSLLPAGIVAVEGEWRRGQTVAIVAPDGQTIACGLCQYAAHEVAQIKGSRTSDIESILGYSYGAEV",
    "response#2": "MRIVVKLGTSVLTDGTDRLHRPRMVDLARQMAQLREAGHEVVLVSSGAVLAGWERLGFPKRRRELTHKQALAAVGQGRLMHIYGQLFEIYDVPVAQTLLTRADLRDRVRYLNARATLLTCLEIGVLPIINENDAVAVDEIRVGDNDTLSALVANLVDAQLLVILSDIAGLYSADPRHNPEATLIDDVAAVNEQVYALAGAAGSHRGTGGMFTKIQAAELATRAGTTMVIEPGSADAELARLAAGGTILIDAGAALALTDKGASLLPGGVAGAEGDFRRGDMVEIHLRDSVGSRCLARGVSQYSALDVRRIARRHSREIEPIGRLLLDDGAALAIRERGTSLLPVGVSAVEGDFLRGEAVACVDAAGHEVARGLVNYSSDEARQIMRKSTREIEAALGYIVEPELLFGAVTVDVGAAAKLRQEGKSLLPIGMVAVEGEFSRGDVIAVRDAAGAEIARGLANYASAEARLLCRRPSSEFERLLGYSAEPEMAHIAVDAGAVQALTQQGRSLLPAGIVAVEGEWRRGQTVAIVAPDGQTIACGLCQYAAHEVAQIKGSRTSDIESILGYSYGAEVAHIAVDAGAVQALTQQGRSLLPAGIVAVEGEWRRGQTVAIVAPDGQTIACGLCQYAAHEVAQIKGSRTSDIESILGYSYGAEVVRAHIAVDAGAVQALTQQGRSLLPAGIVAVEGEWRRGQTVAIVAPDGQTIACGLCQYAAHEVAQIKGSRTSDIESILGYSYGAEV",
    "response#3": "MRIVVKLGTSVLTDGTDRLHRPRMVDLARQMAQLREAGHEVVLVSSGAVLAGWERLGFPKRRRELTHKQALAAVGQGRLMHIYGQLFEIYDVPVAQTLLTRADLRDRVRYLNARATLLTCLEIGVLPIINENDAVAVDEIRVGDNDTLSALVANLVDAQLLVILSDIAGLYSADPRHNPEATLIDDVAAVNEQVYALAGAAGSHRGTGGMFTKIQAAELATRAGTTMVIGTILIDAGAALALTDKGASLLPGGVAGAEGDFRRGDMVEIHLRDSVGSRCLARGVSQYSALDVRRIARRHSREIEPIGALTLDDGAVRAVIKQRRSLLPAGITSVTGRFHGGDVVDLRALDGHTVARGVVAYDQAELASIIGRSTHELPVEMRRPAGTLWVDDGAARALAAGSSLLPPGVRTVEGDFQRGAAVRVVGLDGSPLAKGLIAYPSDEARLLVGHRSGDIEALLGYRGREELMLPQGEMLF"
  },
  {
    "reference": "MNFKAIILAAGKGTRMKSSLPKVVHKVCGKEMVNHVINVSKDSGVSEIVAILGHGSEVVKDVLPEETKIAMQTEQLGTGHAVMMADQYITENDTIVVLCGDTPLVNSDTLENLFKYHLDNGYHATVLTTKVENPTGYGRIIRDNNQDLLKIVEQKDANEEEKLVNEINSGIYCFNGKSLKEALSKIDNNNAQGEYYLTDTIEIMRNNSLKVGAFNGATIEELMGVNSRVELSKAEEIMRRRINTMHMINGVTIIDINSTYIEADVKIGNDTIVYPGAMIQGNTVIGKECIIGMNSCISNSIIGDATKVESSTIMDSKVGDNTTVGPYAYLRPKSNIGNNVKVGDFVEVKNATIEDNSKASHLAYIGDAHVGKNVNIGCGTVFVNYDGKNKFKSVVKDGAFIGSNSNLVAPVTVEENGYIATGSTITDDVPAGALAIARERQVVKEGWVEKKNLKK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The Proton acceptor of the protein must be engineered to enable effective catalysis of acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.2. The designed protein should interact with enzymes and substrates within the Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D- glucosamine biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway to enhance or regulate its activity.3. The presence of Nucleotidyl transferase domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "AIILAAGQGTRMKSKLYKVLHPVCGKPMVEHVIDQVRSLDIKDAVTIVGHGAENVKARLGGKCEYALQEEQLGTAHAVLQSKHILGGKEGLTLVVCGDTPLVTAQTMEALFLHHEKSKAKATVLTACTANPAGYGRIVRSEEGFVEKIVEHKDASEEELQIGEINTGTYCFDNQALFEAIENVSNDNAQGEYYLPDVIEILKKQGEIVSAYITENFDETLGVNDRVNLAIAETVMKKRINEKHMRNGVSIIDPDHTYISAEAIIGKDTVIYPGTMIQGDSVIGEECIIGPNSEIKDSKIGNQTTIRQSVAHDSEIGDSVAIGPFAHIRPSSTISDDVKIGNFVEIKKSVMGKGSKASHLSYIGDAEVGADVNLGCGSITVNYDGKNKFLTKIEDGAFIGCNSNLIAPVTIGKGAYVAAGSTVTNDVPEKALSVARSRQVNKENYVNKLNKKR",
    "response#2": "VIILAAGKSKRMKSDLPKVLHPVAGRPMLQHELDLVSKLDPVEIICIVGHQQDLVRDAFKHYPKVVFVEQREQLGTAHAAAQAKDRLKDFKGDVLIINGDLPLIRPATLERFYTHFRATKAPASLALLTVTLDDPTGYGRVLRDASGAVHGIVEHKDASESQRSIREVNTGILVAPTAALRGWLARLSNDNAQGEYYLTDVIALAVADGVPVATAQPAAPSEVEGVNSRAQLAALERAFQLRQAEALMLAGVSLINPANTYIGVDVKIGSDTVIQPGVMLEGNTVIGEDCHIGPNSRITNSTIGDRTKVDSSVVDSSVVGSDVAVGPFAHIRPATNVGNRAKVGNFVELKKTTLGEGSKASHLTYLGDAEIGTDVNIGCGTITVNYDGVNKFKTIIGDRSFIGCNANLVAPVTINSDTYVAAGTTITKEVPPFALAIGRSRQRNIAGWQRPKKK",
    "response#3": "VIILAAGKSKRMKSDLPKVLHPVAGRPMLQHELDLVSKLDPVEIICIVGHQQDLVRDAFKHYPKVVFVEQREQLGTAHAAAQAKDRLKDFKGDVLIINGDLPLIRPATLERFYTHFRATKAPASLAILTVELADPTGYGRIVRDDAGNIQGIVEHKDASDEQRKIREVNTGIMVIPNHRLSEWLARLDNNNAQGEYYLTDIVAFANQDGVPVHTAQPAHAWETEGVNNKLQLATLERAFQLRQAETLMVNGVSLINPANTYIGVDVKIGSDTVIQPGVMLEGNTVIGEDCHIGPNSRITNSTIGDRTKVDSSVVDSSVVGSDVAVGPFAHIRPATNVGNRAKVGNFVELKKTTLGEGSKASHLTYLGDAEIGTDVNIGCGTITVNYDGVNKFKTIIGDRSFIGCNANLVAPVTINSDTYVAAGTTITKEVPPFALAIGRSRQVTIDGWQRPVKKA"
  },
  {
    "reference": "MYKNQLQELAQRSCFNLPSYTCIREGPDHAPRFKASVNFNGEIFESPTYCSTLRQAEHAAAEVSLNVLSSRVPSKSLTAKILDETGIYKNLLQETAHRAGLDLPMYTSVRSGSCHFPGFSCTVELAGMTFTGESAKTKKQAEKNAAIAAWSSLKKMSSLDSQDEEKEQEAVARVLSRFKPKEVRRRETTNQWRRRTSQQDSNKDLLIERLRWINLLTNQASSSSSTSTPNQHKNSSFISLIPPPPPPKSSKILPFIQQYKDRSSQEAKTETATEMINSKAKVNETSTRLSKQMPFSDMNRYNFVGGCSVNPYSLAPAVQMRSVIPVFAAPPPKPNPNLNPSSLSSSVNEFTSSNNSCSVLNTPGLGGQEKKNLTREMIKLGSESRILDQTHDS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must be able to double-stranded RNA binding.2. The protein must exhibit the following characteristics: Binds double-stranded RNA; May be involved in RNA-mediated silencing..3. The designed protein should contain one or more DRBM domains that are essential for its function.4. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "LSSGTKCISGEHISDQGLVVNDCHAEIVARRAFLHFLYSQLELHLSKHQEDPERSIFIRLKEGGYRLRENILFHLYVSTSPCGDARVNSPYEITTDLNSSKHIVRKFRGHLRTKIESGEGTVPVRGPSAVQTWDGILLGEQLITMSCTDKIASWNVLGLQGALLCHFIEPVYLHSIIVGSLHHTGHLARVMSHRMEGIGQLPASYRQNRPLLSGVSHAEARQPGKSPHFSANWVVGSADLEIINATTGKRSCGGSSRLCKHVFSAWWARLHGRLSTRIPSHGDTPSMYCEAKQGAHTYQSVKQQLFKAFQKAGLGTWVRKC",
    "response#2": "LSSGTKCISGEHISDQGLVVNDCHAEIVARRAFLHFLYSQLELHLSKHQEDPERSIFIRLKEGGYRLRENILFHLYVSTSPCGDARVNSPYEITTDLNSSKHIVRKFRGHLRTKIESGEGTVPVRGPSAVQTWDGILLGEQLITMSCTDKIASWNVLGLQGALLCHFIEPVYLHSIIVGSLHHTGHLARVMSHRMEGIGQLPASYRQNRPLLSGVSHAEARQPGKSPHFSANWVVGSADLEIINATTGKRSCGGSSRLCKHVFSAWWARLHGRLSTRIPSHGDTPSMYCEAKQGAHTYQSVKQQLFKAFQKAGLGTWVRKC",
    "response#3": "LSSGTKCISGEHISDQGLVVNDCHAEIVARRAFLHFLYSQLELHLSKHQEDPERSIFIRLKEGGYRLRENILFHLYVSTSPCGDARVNSPYEITTDLNSSKHIVRKFRGHLRTKIESGEGTVPVRGPSAVQTWDGILLGEQLITMSCTDKIASWNVLGLQGALLCHFIEPVYLHSIIVGSLHHTGHLARVMSHRMEGIGQLPASYRQNRPLLSGVSHAEARQPGKSPHFSANWVVGSADLEIINATTGKRSCGGSSRLCKHVFSAWWARLHGRLSTRIPSHGDTPSMYCEAKQGAHTYQSVKQQLFKAFQKAGLGTWVRKC"
  },
  {
    "reference": "MSQLDSRSASARIRVFDQQRAEAAVRELLYAIGEDPDRDGLVATPSRVARSYREMFAGLYTDPDSVLNTMFDEDHDELVLVKEIPMYSTCEHHLVAFHGVAHVGYIPGDDGRVTGLSKIARLVDLYAKRPQVQERLTSQIADALMKKLDPRGVIVVIEAEHLCMAMRGVRKPGSVTTTSAVRGLFKTNAASRAEALDLILRK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. Ideal protein should have a binding affinity for key enzymes in the 7,8-dihydroneopterin triphosphate biosynthesis pathway to enhance the overall efficiency of the pathway.2. The designed protein must be able to regulate tetrahydrofolate biosynthetic process, one-carbon metabolic process. The designed protein sequence is ",
    "response#1": "MIIGIWAEDEAGLIGEADKMPWSLPAEQQHFKETTMNQVILMGRKTFEGMNKRVLPGRISIILTRDETYQSDNEKVLIMHSPKEVLDWYHKQNKDLFITGGAEILALFESELELLYRTVVHEKFKGDTYFPSTFDFGRFKLVSEKFHDKDERNSYTFTIKKYEGK",
    "response#2": "PEKDIDGFHPANFGRMTLGQKAFRPATAYGICKLLQFYEVPVKAKHCVVIGRSNIVGKPISIMLSNDFDIGNATVTLTHIETPRELLLDETRRADIVIVAVGIPGFVTADMVKDGVVLIDVGINRLESGKIVGDVDFENVAQKCSWITPVPGGVGRMTVAALMINTLMAYR",
    "response#3": "IEQTIYQLLELLGEDPNREGLLDTPKRVAKMYLEMFNGLEEDPKDQFTAVFSEGHEEVVLVKDIPFHSMCEHHLVPFYGIAHVAYIPSKGRVTGLSKLARAVEVASRRPQLQERLTHQVAHALQDALEPEGVFVMVEAEHMCMSMRGIRKPGSKTVTTVALGKYKEDAILRRELLSML"
  },
  {
    "reference": "MSVNKHEQGPAKARPAPPRPARTEPVPVTTLVARSGARLIAGPANSAPPETAVTGATLRAQDVRPGDLFAALPGARVHGADFAGDAIAAGAAAVLTDEAGAERPDLRDTELPVLVHPDPRAVLGEIAAWIYGEPSLRLSVLGVTGTSGKTTTAYLVEAGLRAAGHDTGLIGTVETRIGGERLASAFTTPEAPDLQALFALMAEQGVTHVPMEVSSHALSLGRVNGTRFAVGAFTNLSQDHLDFHRDMEEYFAAKSLLFDGRSTSEVVVVDSAWGQQLVTPQTVTVATEPGLDASWRADDIATTPTGEQAFTLHHPDGRTARASIPLPGEFNVANAVLAAAILDTVDVPLDTIVAGLASVEVPGRMERVSVGQPYTAVVDYAHKPAAVTEALRALRARTDGRIITVLGCGGDRDTAKRPMMGEAAVRGSELVIVTDDNPRSEDPAAIRAAMLQGARAVSPAEAGEIREIGDRRAAIAAAVAEAGPGDVVLVAGKGHESGQDVGGVVHPFSDRDELEAAIRKTLEVNL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein contains novel Mur ligase central, Mur ligase C-terminal, Mur ligase N-terminal catalytic domains that confer a unique function or activity.2. The ideal protein would be one that plays a critical role in cell wall organization, regulation of cell shape, cell cycle through magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity within cytoplasm.3. The protein requires Mg(2+) to activate a critical catalytic site.4. The designed protein should be able to compete with endogenous proteins or inhibitors for meso-2,6-diaminoheptanedioate, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP binding.5. The designed protein should interact with enzymes and substrates within the Cell wall biogenesis, peptidoglycan biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MRLDSRSVLLDDLFVAVHGHSTNGHHFISQAINQGAAAVIAETTEKRNHGKINHIQGIPVIYIMNLSHCLSAIAGRFYGNPSRELWMAGITGTNGKTTITYLLEEILTGLGYAVGVIGTVNYRYTAAGEKAVLPASHTTPDAMHLQELLRRMVDAGISHVIMEVSSHALAQARIGNIQFDVAAFTNLTRDHLDYHADMYEYFETKARLFTHHLKAAGNAVIGYPQGTAEEGGWSGMLALQCRDRGIRALICGAHPEADIRLTGFEADLRGNRMTVATPDGGHSLHSPLVGRFNADNVMVALAVCQALGFELSAIAGGRFETVPNDKGISVIVDYAHTPDALENVLNTAREFVEGDLIAVFGCGGDRDSEKRPLMGGIGQRLSDRCIITNDNPRTEDPELIIKDILAGLDESNENYKVVMDRKEAIKEAIESAKKGDVILIAGKGADAFQIVKGQQVEYSGSAEVVRELLG",
    "response#2": "MKLRDLLQGIAPVSLKGDRDISISGIEFDSRKVEVGSLFVAVKGYKADGHDYIDKAVASGASAVICEEVPDGLGCVVVADPRLAMGLAAAHFYGHPTKKLKLIGMAGTNGKTSVTWMLESILRSAGLKSGVIGTINYRYKNRVQEAKNTTPDLVTLQRLLAEMVEAGVEYVVMEVSSHALVQERVAGLVFDVGVFTNLTQDHLDFHKTMEAYYQAKAKLFEMTAAGKGVINMDDEYGQRMLNAVPSHTYGFSPEADVWAADIKMDAQGIHFRLATPWGESMVKTNLVGRFNVYNTLAAITACLAEGVSLEAISAAAEEVKGVEGRMQLVTGHPSGARVIVDYAHTPDALRAALSALRPEAKGRLAVLFGAGGDRDPGKRSMMGKVAQNGADLVYVTDDNPRSEDPGDIRKAILDGCPDAVEIADRGAAITAALGELSAEDVLLIAGKGHETYQILGEKTIHFDDREVARQALQERL",
    "response#3": "MTLDSREAGPGVLFVAVAGAHVDGRAFISQALASGVDAVLCDADTMPEDVPLQDARVLGVPGLRGRLSRELGSPILTGSCGKTTVKEMTAAILARHWPPGPDNPVDSVLKTTGNFNNLIGMPVSLLPLTVRHRAAVIEMGMNRFGEIARLAAIAEPDISCITNIHAAHLEGLHSIEGVARAKEELFAGTSPDGILVVNVTGTNGKTTIAQLLAQWTQLLRHKSAVMGTIGNGILGQVKAAANTTGSAVEIQSSLADFVQQGVDFAAIEVSSHGLAQHRVEALTFQVGIFTNLSRDHLDYHLTMENYAEAKKRLFSELQTRYQVINADDPIGEQWLAELPEAVAVSCRADFQANHTAWLKATSVNFTQQGAIIEFASSWGNGTLHSQLIGAFNVSNLLLVAACLDAVGVPLEIAIRRLGEFENVPGRMQLVTGHPSGARVIVDYAHTPDALRAALSALRPEAKGRLAVLFGAGGDRDPGKRSMMGKVAQNGADLVYVTDDNPRSEDPGDIRKAILDGCPDAVEIADRGAAITAALGELSAEDVLLIAGKGHESYQEIRGVKHPFSDVEEALLALERTCAA"
  },
  {
    "reference": "MSTKIDGKQIAAEIKTNLAERVNRLKAQGIQPGLGTLLVGEDPGSMKYVAGKHADCQEVGITSVKKELPADASFDDIAATVRELNEDPACTGFIVQLPLPKGINENAIIDMIDPAKDADGMHPYNLGELVLHVRGDISTPLPCTPRGVLELLDAYDIDLNGKEVCVLGRGITIGRTIGLMLTRNAVNATVTLCHTGTRDVADHMRRADVIVAAMGSAGFVTPDKIKDGAVLVDVGISRVYDEEAGRYRIKGDVDKACYDKASAYTPNPGGVGPMTRAMLLANVVEMAERHA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein with methenyltetrahydrofolate cyclohydrolase activity is needed to enhance methionine biosynthetic process, purine nucleotide biosynthetic process, histidine biosynthetic process in a specific context.2. A protein that is capable of catalyzing reactions within the One-carbon metabolism pathway with high efficiency and specificity.3. The protein should have a low Km value for 5,10-methenyltetrahydrofolate + H2O = (6S)-10-   formyltetrahydrofolate + H(+). The designed protein sequence is ",
    "response#1": "IEKDIDGLSSYNLASNYNCKDNFLSATPKGIILLLKHYNINFRSTVIALVGQSNIVGKPLAKYLSNFKNNTIKTYVKDTPKDDLHEAKIVIVATGVYQSVTADQLANGTVLIDVGIHRQGKTIHGDLDFASCFKKASYITPVPGGVGPLTVVALMFNLIKG",
    "response#2": "IDGSRASARIREQLKERVACLRARNIRPGLAVLLVGEDPASQVYVRNKVAACEQVGIHSVTERYPSAWTENQLLDRIASLHGDPAINGILVQLPLPPHMSAHRVIEAISPLKDVR",
    "response#3": "IDGSRASARIREQLKERVACLRARNIRPGLAVLLVGEDPASQVYVRNKVAACEQVGIHSVTERYPSAWTENQLLDRIASLHGDPAINGILVQLPLPPHMSAHRVIEAISPLKDVR"
  },
  {
    "reference": "MTLPFADSAQSTLGIEWELALVDAVSGELRSEAPDLLRALHVAEGLADDDVNPHMTSELLQNTVELVTGVHERVDAATADLGRIAARVADAAAARGISLFCQGTHPFADAIAQPSTPSERYDRMLDLTQYWGRQLLIFGVHVHVGLDDVSKAMPVVNGLVNRVPHLLALSASSPFWAGTDTGYQSQRTLLFQQLPTAGLPFQFQEWEDFERCVAQMEQVGMIADVTECRWDVRAVPRLGTVEMRACDGLATLEEIAAVTAYTQCLVDDLSASLERGETVEVLPPWHAQENKWRAARYGMDATVIVDARGTQVPLAEHLPAEIERLTPVAERLGCEAELAGVQAMIDDGGAARQRRVEAQALAGPPAEGEDADDAVAPLRAVVLDAAARTRASLDGRTG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must have high specificity towards glutamate-cysteine ligase activity.2. For general function, the protein need meet that ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity. The designed protein sequence is ",
    "response#1": "PRIVAADDWAVLGRGLGQRVRALEAFLHDVYGERAAVADGIVPPWVVNDAPGLRHGGRAPGRDAIRITTAGIDLVRGGDGGWLVLEDNLRVPSGIAYAVEGRRLAESVLPELGPPPGILRLGTVPALLHDALVAAAPPAVTGEPAVAVLTSGPADSAYFEHAMLAEEMGVPLVEPGALLVDDDVAYRVDDGRRRRVDVLYRRIDEDELFAAPGADGAPLGPALLRAVRAGQVSVANAPGNGIGDDKVVYAYVPRMVTYYLGEQPVLDDVPTYVCGDPEQCEHVLANLDQLVVKPVDGFGGSGVVIGPHAEPYQLTAVREQILADPRRWIGQEVVALSTHPTWHDSHLEPCAVDLRVFVYAGVEPVVVPAALSRVR",
    "response#2": "PRIVAADDWAVLGRGLGQRVRALEAFLHDVYGERAAVADGIVPPWVVNDAPGLRHGGRAPGRDAIRITTAGIDLVRGGDGGWLVLEDNLRVPSGIAYAVEGRRLAESVLPELGPPPGILRLGTVPALLHDALVAAAPPAVTGEPAVAVLTSGPADSAYFEHAMLAEEMGVPLVEPGALLVDDDVAYRVDDGRRRRVDVLYRRIDEDELFAAPGADGAPLGPALLRAVRAGQVSVANAPGNGIGDDKVVYAYVPRMVTYYLGEQPVLDDVPTYVCGDPEQCEHVLANLDQLVVKPVDGFGGSGVVIGPHAEPYQLTAVREQILADPRRWIGQEVVALSTHPTWHDSHLEPCAVDLRVFVYAGVEPVVVPAALSRVR",
    "response#3": "PRIVAADDWAVLGRGLGQRVRALEAFLHDVYGERAAVADGIVPPWVVNDAPGLRHGGRAPGRDAIRITTAGIDLVRGGDGGWLVLEDNLRVPSGIAYAVEGRRLAESVLPELGPPPGILRLGTVPALLHDALVAAAPPAVTGEPAVAVLTSGPADSAYFEHAMLAEEMGVPLVEPGALLVDDDVAYRVDDGRRRRVDVLYRRIDEDELFAAPGADGAPLGPALLRAVRAGQVSVANAPGNGIGDDKVVYAYVPRMVTYYLGEQPVLDDVPTYVCGDPEQCEHVLANLDQLVVKPVDGFGGSGVVIGPHAEPYQLTAVREQILADPRRWIGQEVVALSTHPTWHDSHLEPCAVDLRVFVYAGVEPVVVPAALSRVPRIVAADDWAVLGRGLGQRVRALEAFLHDVYGERAAVADGIVPPWVVNDAPGLRHGGRAPGRDAIRITTAGIDLVRGGDGGWLVLEDNLRVPSGIAYAVEGRRLAESVLPELGPPPGILRLGTVPALLHDALVAAAPPAVTGEPAVAVLTSGPADSAYFEHAMLAEEMGVPLVEPGALLVDDDVAYRVDDGRRRRVDVLYRRIDEDELFAAPGADGAPLGPALLRAVRAGQVSVANAPGNGIGDDKVVYAYVPRMVTYYLGEQPVLDDVPTYVCGDPEQCEHVLANLDQLVVKPVDGFGGSGVVIGPHAEPYQLTAVREQILADPRRWIGQEVVALSTHPTWHDSHLEPCAVDLRVFVYAGVEPVVVPAALSRVR"
  },
  {
    "reference": "MIELRHEVQGDLVTINVVEHPEDLDGFRDFIRAHQNCLAVDTETTGLDIYSADFRCRLVQFGTQTESWVLPIEDLEMLGREEVHEALDVLNKIVMQNASYDLQVLDRCFGIKMESLWPKILDTQILAKLVDPRPFGAGGFGHSLEELIAEFISKEQAENVKGLMAKLAKEHKTTKAKIWSTIDLYHPEYLTYAGMDTVFTARICSALAPLVPDVSRPLVPYEHKISEICSYIDRRGFLLDVEYSQQLADKWLGEQQVWEAVLLNEYGIEKVNATEDVAEAFEELGHKFTAFTDSGKRKVDKGFYADMIAAGGEKAHLAEMVQEAKKLGKWRTSWVQTFLDTRDSEDRCHTFVNPLQARTSRMSITGIPAQTLPASDWTVRRCFLADPGHVMASIDYQAQELRVLAALSGDRTMIQAFKDGADLHLMTARAAFGDHITKDDPERKYAKTVNFGRVYGGGANTVAEQTGISIETAKQVVDGFDKAYPGVTRYSRKLANEAKRNGYIINPMGRRLPVDRSRTYSALNYQIQSTSRDVTCKALIRLHEAGFTPYLRLPIHDEIVASLPAEKANWGAREIARLMAEEMGPVLIGTDPEVGKRSWGSLYGADY",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Replicates viral genomic DNA; This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'-5' direction..2. We are looking for a protein with broad substrate specificity for a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =   diphosphate + DNA(n+1).3. The designed protein must possess DNA binding, 3'-5' exonuclease activity, DNA-directed DNA polymerase activity to support DNA-templated DNA replication, viral DNA genome replication under certain conditions.4. The designed protein should contain one or more 3'-5' exonuclease domains that are essential for its function. The designed protein sequence is ",
    "response#1": "ERMSVEEAIELSREYGVPFHPKYTFLWHDVKPKDVDELREALEVAGSEWGNLRVEFENDGEIKRILEDLLVPHRLEDDTIVVEEPWASALLAQLGYDPESGEFREQDEDLDYLLDYLVIRDETCRYVSKLAGFPIREKAPTRIGARMGRPEKARERKMSPPPHVLFPIGIAGGNQRDIMKFHRGESEDTDRVEVCYRICPECDRLVPYRVCPFCGTETVV",
    "response#2": "ERMSVEEAIELSREYGVPFHPKYTFLWHDVKPKDVDELREALEVAGSEWGNLRVEFENDGEIKRILEDLLVPHRLEDDTIVVEEPWASALLAQLGYDPESGEFREQDEDLDYLLDYLVIRDETCRYVSKLAGFPIREKAPTRIGARMGRPEKARERKMSPPPHVLFPIGIAGGNQRDIMKFHRGESEDTDRVEVCYRICPECDRLVPYRVCPFCGTETVL",
    "response#3": "VRTDFSLGESALKAENAVKIARDAGYTAVISADSMNIASVIPLQRAAGDDMAVICGVKLNVVDDPTYEHRARLAKESERCMESLVRDRSYCFTALIKNEQGYRDVCELMTLANKREQFYFVPRLALDQLAAAYAKGNIILLTSDIGSVFQY"
  },
  {
    "reference": "MAPESKNAGVSVVVNLDSDSDSDNDDGVGGRGAFRSMASLMDKHQVPSTTADATVAPRENLECRSFWKAGENFVIPTGVTNPAAPAIAELIDNAVDEIQNGATFVKIDKINIVKDNSPALVFQDDGGGMDPDGLRKCMSLGYSSKKSNTTIGQYGNGFKTSTMRLGADDIVFTRSTRGGKSTQSVGLLSYTFLRKTGQDDVVVPMIDIDTSKERPQPIIYGSAEDWAASLEIILKWSPFSTEGELWQQLEDIGTHGTKVIIYNLWLNDEGIYELSFHDDNEDIRLRDESVHDSKRVHHNLLELRSHISYHLRYSLRAYASMLYLKRFNNFKIILRGIPVEQFNIADELRLPETIKYNPHTTKEKAPTEIKVGFIKEAPKLAVCGFNVYHKNRLIRPFWKVTMGGERRGSGVVGVLEANFIEPAHDKQDFERSSLFQRLEARLKKIVSNYWNTHCHVFGYCTYGMPADKSKRIAIPDQPPTVNTFNPLPLPSDEIRVSQGGPIIREISLSNATSSRIAAVDTENNLVGKSAHEISEENIQLFMRCEEYVKKETELEQTVSNLAKELEETKSKCARLALLVDAKRREMQQV",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that May also be involved in the regulation of chromatin architecture to maintain gene silencing.; Probably involved in DNA repair; Involved in RNA-directed DNA methylation (RdDM) as a component of the RdDM machinery and required for gene silencing; Binds DNA/RNA in a non-specific manner and exhibits endonuclease activity; Exhibits ATPase activity2. The designed protein should have ATP hydrolysis activity, RNA binding, endonuclease activity, DNA binding, ATP binding within nucleus to regulate chromatin organization, DNA repair, positive regulation of response to biotic stimulus, regulation of DNA repair, regulation of gene silencing by RNA.3. The protein must contain a signal peptide for proper functionality.4. Mn(2+), Mg(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "MWIQVRTMDGKVAHTVDSLSRLTKVEELRKKIQELFHVEPGLQRLFYRGKQMEDGHTLFDYDVRLNDTIQLLVRR",
    "response#2": "QRVWAKRVFMNQSFAIVAPTGVGKTTFGLVMSLFLKGRVLAIFPTRLLAQQAGDRLSELAQKVGVNKKILIYQSKKNIREQFLNGDWDILLGTNMFLHKNFENLINFKFKLIFIDDIDSFLKRGKNVDYLFKLY",
    "response#3": "LNEEQQRILAACQTGQSLFFTGSAGTGKSFLLRKIIGALPPDGTVATASTGVAACLIGGTTLHSFAGIGGGEATLPNCYEKASRPNTAQTWRKCKRLIIDEISMIDADYFEKIEAVARYVRKNDKPFGGIQLILCGDFFQLPPVGRQSDRARPSKYSPDADSEGDFGGVRFCFESPVWSDCIQASYELTIVHRQRDQEFVSILNSIRIGRVTR"
  },
  {
    "reference": "MAKHIFVTGGVASSLGKGLTAASLGRLLKCRGLRVTMQKLDPYINVDPGTMNPYEHGEVFVTEDGGETDLDLGHYERFIDEPLTRDSNATTGSIYQEVLAAERRGDYLGRTVQVIPHVTDEIKRRISKLAGDDVDVVITEVGGTVGDIEILPFLEAIRQMRLSVGRSNVCYVHVTLVPFIGPAGEQKTKPTQHSVTELRSRGVQPDAVVLRSQVEIADSLRSKISDLCDVDLAGVVNAPDAANLYEIPMILHREGLDNVVCDILGFDHLEPDLAEWQQMLDRIDRPESTVRVGVVGKYVALQDAYLSVAEALRHAAGFQSAEVEIEWIEAEEAVDLLSAGRLHRLDGMVIPGGFGERGIEGKIAAAAYARANEVPCLGLCLGMQVMVIEYCRNQLNLPDANSTEFDEHTAHPVIDIMESQRGIEDKGGTMRLGLYEAHLAPGSRVAGLYGATVVHERHRHRYELSEKYREAMDEQGLVSSGLSPDGTLAEFVELAGHPFWIGTQGHPEFTSRPNRPAPLFRGLLEAAMVRAEGRNPHLLRPPPEDVVPADDR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must have at least one Glutamine amidotransferase, CTP synthase N-terminal domain.2. The Nucleophile; for glutamine hydrolysis, active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. A protein with metal ion binding is needed to enhance 'de novo' CTP biosynthetic process in a specific context.4. The ATP, Mg(2+), UTP binding site should be located in a region of the protein that is accessible to the ligand.5. The protein should be optimized for activity in Pyrimidine metabolism, CTP from UDP: step 2/2, CTP biosynthesis via de novo pathway pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "LHDAYLSVMESLKHAGWENQSRVSISWIDSECVTPDSVDDLLAGSDGILIPGGFGDRGIEGMIVAARYARENKIPYFGICLGMQIAVIEYARDVMGLVDANSSEFAPNGLHSVIDLMPDQMGNIPKGGTMRLGEYPCAIVPETIMNRAYRGEAEIKERHRHRYEFNNVFREQLVKAGLTISGHSPDGHLVEAVELPEHVHPFYLGVQFHPEFKSRPNRAHPVFREFLVRFTQQHT",
    "response#2": "LHDAYLSVAEALSHGGIANDARVDIKWVHSEDLEEDGIDLEEIFSGVDGLLIPGGFGDRGIEGKIKAIRYAREKKIPLFGICLGMQCAVIEFARSVCGLQGAHSTEFDPNTPHPVIDLMPEQKDIEKKGGTMRLGHYPCRITPGTRTMEAYGEEMIEERHRHRYEFNNEYREVLEKAGMVISGLSPDGRLVEIIELKDHPWFVGTQFHPEFKSRPNRPHPLFRDFIINAMKFAGGKLKDAYISHEHTFMHVTGKTGIGVKIKWVNSEKEENNLEILRDVDGILIPGGFGPRGIEGKINAIRYARENGIPLLGICLGFQLSVIEFARNVLGYDGANSTEFNPNTKYPVIDLLPEQLGVSEMGGTMRLGDLKVNIEKGTHAFQLYGKSEIYERHRHRYEVNPLFIKELEKEGMKFSAKDQSRVRMEILELKGHRFFMGSQFHAEFKSRPMKPSVLHEALVEFGVQIN",
    "response#3": "KYIFVTGGVVSSLGKGIAAASIGCLLESRGLRVTLQKFDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTYAPLTQSNNVTSGRIYETIISRERRGDYLGKTVQVIPHVTDEIKANIEKVSGGVDVVLVEIGGTVGDIESLPFLEAIRQLRHKKGRENCFFVHVTLVPWIAAAGELKTKPTQHSVKELRTIGIQPDMLICRCEKPLAGDIKDKIALFCDVSADAVIEACDAKSVYEVPLGFASQNVDDIILRQFKLPTTKANLKEWEDVVYRLEHPKHEVTIAFVGKYLDLKESYKSLTEALIHAGAHINAKINIHWCDSERIEDAGAYEVIQTCDAVLVAGGFGHRGVEGKLKAIEYARTNNIPYLGICLGMQLAVIEYARNVLGIEDANSVEFDPEAKNPLIYLIDEFLDQSGNKQVRTHESPMGGTMRLGEYPFTPKKGSKLQKAYGDKETYYERHRHRYEANPKYKEALEKAGMIISGESHGLIEAVELKDHKWFVGVQFHPEFTSHLETPNPIILEFVKAALKYRK"
  },
  {
    "reference": "MTAPFVPAAAHVRRFLKSSAGGAFFLLLASLAGFVLANSPWAAGYRTLTTLPLKFPFLGKRGPDNVAAWVSDGLMTLFFLVVILEIKKEIVSGHLSSLRRVALPLIGAVGGMVVPALTYLLVTWGHPEATSGWAIPVATDAAFTLPIILALGRRVSPGARAWLMALAIFDDVLGIVVIALFYGGSMYWPALLAVVLVTAALIGANRGRIRTLWAYGTGGILLWTALLDSGLHPTLAGVITGLCLPAGDAKGAATLDWVSSAVTPLVTWIVLPLFGFMNVGMSAAGMKPDMMLEAVPLGIMLGLMLGKPVGVFGATLLSIRLKVVMLPAGTSTGMLFGLSLLCGIGFTISLFVANLAFSGSDLIAPAKMGIFAGSALSALTGWFWLRFMPQNVTRPSEEGGFPR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein have enhanced 2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out).2. A protein that can perform sodium ion transmembrane transporter activity, antiporter activity in plasma membrane to support regulation of pH.3. The protein should have Helical domains to ensure proper insertion into the membrane. The designed protein sequence is ",
    "response#1": "MLRKLTFLLS",
    "response#2": "MF",
    "response#3": "MASSS"
  },
  {
    "reference": "MAPMGIRLSPLGIAVFCLLGVGVIYHLYAGVLSSRLAFFRQKRTVDLRELLALSIDAAVQGGREVKRIREDNTLEEKSKGKTKEGASEKYTLGDLNSHRKMYYLIKNTFPNIQVNSEEHANAEGEATVWTRMIPEDILAKVSGGKEIPAEKITVWIDPLDATQEYTENLLKYVTTMVCVAVDGEPVIGVIHKPFTGYTVWGFVGEGSNVAPRDSYNTNSPKVIVSRSHAGKVKSFVQTAFGNNTEIIPAGGAGYKALALLNPTDDKQETADIYIHVTYIKKWDICAGDAILKSLGGQMTTLKGEQIDYSGLEGNKGGLLASMKVDHKALVKRLPLWEDNKQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein should be able to manipulate the activity of enzymes in the Polyol metabolism pathway, either by activating or inhibiting their function.2. The protein should have a high affinity for Mg(2+).3. The designed protein should be involved in inositol phosphate dephosphorylation by localizing to membrane and exhibiting inositol monophosphate 3-phosphatase activity, metal ion binding. The designed protein sequence is ",
    "response#1": "QFSLENATIGVPKQVVVNEPKSFDLHKFEEEQGLRFPVIAKPLAADGGAGSHELCLVFDEEGLHALSVPMVLQEFVNHGGVVFKIYVAGQRVNCVKRKSLGDITEEKLKVLRGSLPFSRVSSLGVEDEGGGAVEDAEMPPQSLVGELARGLREALGLNLFNVDVIRDGKEPTRYLVIDINYFPGYAKLPSYEPFITDFLLDIVRSKG",
    "response#2": "QFSLENATIGVPKQVVVNEPKSFDLHKFEEEQGLRFPVIAKPLAADGGAGSHELCLVFDEEGLHALSVPMVLQEFVNHGGVVFKIYVAGQRVNCVKRKSLGDITEEKLKVLRGSLPFSRVSSLGVEDEGGGAVEDAEMPPQSLVGELARGLREALGLNLFNVDVIRDGKEPTRYLVIDINYFPGYAKLPSYEPFITDFLLDIVRSKI",
    "response#3": "QFSLENATIGVPKQVVVNEPKSFDLHKFEEEQGLRFPVIAKPLAADGGAGSHELCLVFDEEGLHALSVPMVLQEFVNHGGVVFKIYVAGQRVNCVKRKSLGDITEEKLKVLRGSLPFSRVSSLGVEDEGGGAVEDAEMPPQSLVGELARGLREALGLNLFNVDVIRDGKEPTRYLVIDINYFPGYAKLPSYEPFITDFLLDIVRSKG"
  },
  {
    "reference": "MSQHPDINTNVDATSLTDDQKSLDTNATSGNEVAPDGNKHIRIVNTFMKRRTHMNKNAELALTAPEFAHYLVNNSFGDGNLEGIDDLRALFAELPNGSEAPLTLEIGFGLGDSFIEMAAAEPSRNFVGIEVHEPGIGKCAYMAGTQNLSNVRIINGDAIQLLKQLPENHIDRIQLYFPDPWQKKRHHKRRFVSPERMAIVTRSLKQGGWFHTATDWEHYAFWMVEVLDGFVGLSNQAGAGNFTSRPDFRPMTKFERRGLASGHGVWDLIYLKD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein is the optimization of the active site to achieve high catalytic efficiency for guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-   methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine.2. The substrate, S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions.3. We require a protein that can specifically bind to the metabolites produced in the tRNA modification, N(7)-methylguanine-tRNA biosynthesis pathway.4. The protein must be able to tRNA (guanine-N7-)-methyltransferase activity.5. The protein must exhibit the following characteristics: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA..6. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "MSELKDEEYIWIHCSSVGEVNLSEDLVKKFYSISRKNILISVFTDTGYENAVKKYSDKKKIKVIYFPVDDKKKINEILNKIKLKLLVLVETELWPNLINEVNEKNSRIIVVNGRISDRSYPRYKKLKFLLKSMLQKIAFFYMQSEIDKERIVSLGAIKEKVENVGNLKFLDTSVAEHSIRDIHTFFCGPKPMIKAAAAALKKWGVAEARIHYEFFGPAEEL",
    "response#2": "MKLLKDEEYIWIHCSSVGEVNLSEDLVKKFYSISRKNILISVFTDTGYENAVKKYSDKKKIKVIYFPVDDKKKINEILNKIKLKLLVLVETELWPNLINEVNEKNSRIIVVNGRISDRSYPRYKKLKFLLKSMLQKIAFFYMQSEIDKERIVSLGAIKEKVENVGNLKFVYRANTPEDLEKKIKEAKLLSDEPLVVSVAFTGRGDKDIEAAEKILEEYGYKLEEVEKDDYELRIELKDEEYIWIHCSSVGEVNLSEDLVKKFYSISRKNILISVFTDTGYENAVKKYSDKKKIKVIYFPVDDKKKINEILNKIKLKLLVLVETELWPNLINEVNEKNSRIIVVNGRISDRSYPRYKKLKFLLKSMLQKIAFFYMQSEIDKERIVSLGAIKEKVENVGNLKFEYKRNFEIYSEDEIMEIVAKVIGNRVVK",
    "response#3": "MLKDEEYIWIHCSSVGEVNLSEDLVKKFYSISRKNILISVFTDTGYENAVKKYSDKKKIKVIYFPVDDKKKINEILNKIKLKLLVLVETELWPNLINEVNEKNSRIIVVNGRISDRSYPRYKKLKFLLKSMLQKIAFFYMQSEIDKERIVSLGAIKEKVENVGNLKFDIPPYSGVSYMAFQKDFDNFEKLIKERDTKHFDMETYEDVSKLINSKIIHF"
  },
  {
    "reference": "MSDVITTDQPASATSLLQAKGIANLVERALLTEVRLTPKPGLVDIRNSGAHKDMDLALFEKSTLAVAPWMENFYQLGYDTSALEAELVLPMLRPIGMACEADMLQATGGVNTHRGAVFSFGLISAVTGRMVALEEELEQNRICYWVARMCRDLVAKELSSEATNAATSKSVEHFLHYGLSGARGEAESGFQTVRTVALPIFERIRAQNEDMNLALLQTLLHLMAWNNDTNLVSRGALKGLYYVQQQAQKMLWEGGVLMRGGLEALQAFDDELIARNLSPGGSADLLAVTWFLSHFPKGETFAD",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be designed to have high stability and activity towards 3'-dephospho-CoA + ATP = 2'-(5''-triphospho-alpha-D-ribosyl)-   3'-dephospho-CoA + adenine.2. The protein must have high specificity towards triphosphoribosyl-dephospho-CoA synthase activity, ATP binding.3. For general function, the protein need meet that Catalyzes the formation of 2-(5''-triphosphoribosyl)-3'- dephosphocoenzyme-A, the precursor of the prosthetic group of the holo- acyl carrier protein (gamma chain) of citrate lyase, from ATP and dephospho-CoA. The designed protein sequence is ",
    "response#1": "MTTVAVIDKRAGLTTHPAPGEPGPTLVNHLLHRWPDVASDISGMDRQRPGIVHRLDKDTSGLMVVALTEGDRLRLAADFAGRRVRKVYLAVVLGRPAQPAGTIDAPMGRHPSQKTRMAVVARGGREAVSDYRVLWTGPRGLASLVAVRIHTGRTHQIRVHMAHIGHPLLGDSAYGPRENVEWSRRPDRLAGLAPRQMLHAFHLSSEELLTEHS",
    "response#2": "VAVIDKRAGLTTHPAPGEPGPTLVNHLLHRWPDVASDISGMDRQRPGIVHRLDKDTSGLMVVALTEGDRLRLAADFAGRRVRKVYLAVVLGRPAQPAGTIDAPMGRHPSQKTRMAVVARGGREAVSDYRVLWTGPRGLASLVAVRIHTGRTHQIRVHMAHIGHPLLGDSAYGPRENVEWSRRPDRLAGLAPRQMLHAFHLSEAQGRTRIERVLETLS",
    "response#3": "MFVAVIDKRAGLTTHPAPGEPGPTLVNHLLHRWPDVASDISGMDRQRPGIVHRLDKDTSGLMVVALTEGDRLRLAADFAGRRVRKVYLAVVLGRPAQPAGTIDAPMGRHPSQKTRMAVVARGGREAVSDYRVLWTGPRGLASLVAVRIHTGRTHQIRVHMAHIGHPLLGDSAYGPRENVEWSRRPDRLAGLAPRQMLHAFHLSAAPC"
  },
  {
    "reference": "MPDRSASGVVVIATRNKGKVREFAHYFARFGKEVRSLDDFDDMPDIVEDGATFAANARIKASAIAGRLNLPVLADDSGLCVDALGGDPGVYSARYAGEHAADAANNAKLLRELGAVSAAAARAPGPPDGPEAADAAAPQVLSTARFVCALALVDPLRGDTLEAEGTCEGVIIAEPRGTDGFGYDPLFYLPERGLTMAELPLEEKNAISHRAAALRQFFERHSGRLT",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in dITP + H2O = dIMP + diphosphate + H(+) are properly positioned for optimal catalysis.2. The protein design must be able to perform ribonucleoside triphosphate phosphatase activity, dITP diphosphatase activity, nucleotide binding in order to contribute to nucleotide metabolic process, purine nucleoside triphosphate catabolic process.3. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..4. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate, Mg(2+) binding.5. The protein must bind to Mg(2+) in order to perform its enzymatic function. The designed protein sequence is ",
    "response#1": "MRIVLATTNPGKVRELRAILAEVPGLELVSAGELGVAEAPETGTTFVENALIKARAAAAHTGLAAIADDSGLAVDALGGAPGIFSARWAGRHGDDAANLELLLAQLSDVADPRRGAHFVSAAALVLPDGAEVAEHGRWPGRLARTPAGEGGFGYDPIFVPEGEERTCAEMAPEEKNAISHRGRAFRALAPQVRALLAGG",
    "response#2": "MKIVIATHNEGKIREIKEILKPLGDVEILTLSDFPEIGEVKETGNTFEENAKKKAVEIARYTGKPALADDSGLCVDALGGMPGIYSARFAGEGATDKENNDLLLANMRDVPDDERGASFVCALAIALPDGTEVVVRGECRGRILREPRGAGGFGYDPLFVPEGYEQTLAELPPEIKNKISHRGKALREMRELLKGL",
    "response#3": "MPGPVRVLLATRNPGKARELAALLAEAGFDLEVGPPADLPAVAETGSTFAENARIKARAYAAHTGLPALADDSGLVVEALGGAPGIFSARWAGRHGDDRANLDLLLAQIADVPDAQRGAAFACAAALVLPGGREHVARGEWRGRILRAPRGEGGFGYDPVFLPDGETRTSAELTAEEKDAVSHRGRAFRLLAPELAAILDVR"
  },
  {
    "reference": "MNRQPLTVAVVGATGVVGRTMIDILNEREFPLDELRLLASERSAGQNVSVGGRQVEIGLARPEAFEGVDLALFSAGGDASRELAPAAVARGTTVIDNSSAWRMDPTVPLVVSQVNPGDLEGHPGIVANPNCSTMQMAPVLMALRDAVGLERVIVDTYQSVSGTGSNAVAELEGQVRAHVAGEAPTVSVYPHPIAFNVLPEIDVFLPNGYTKEEWKVVSENRKILGLPDLRISCTAVRVPVFVSHSEAIHVETRNSSTPEQARELFAMVPGVVVQDDPAEHRYPLAVEAAGRDEIFVGRIRRDVSIADDRGLVIWVVSDNLRKGAATNAVELAEILNDRGWIGPATDRGAQPYRALGPVEAFA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein have Semialdehyde dehydrogenase NAD-binding domains with specific sequence motifs that are crucial for its activity.2. The designed protein must possess NADP binding, aspartate-semialdehyde dehydrogenase activity, protein dimerization activity to support threonine biosynthetic process, lysine biosynthetic process via diaminopimelate, diaminopimelate biosynthetic process, 'de novo' L-methionine biosynthetic process under certain conditions.3. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..4. The protein's Acyl-thioester intermediate must be carefully engineered to ensure that the amino acids involved in L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH are properly positioned for optimal catalysis.5. The NADP(+), substrate, phosphate binding site should be located in a region of the protein that is accessible to the ligand.6. The designed protein should interact with enzymes and substrates within the Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5 pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MTLGIVGATGQVGVAVREILLERDFPIEEVRFFASARSAGTVLPFGDRDVTVEDASTADPAGLDIAIFSAGATTSRALAQSFVDAGVVVIDNSSAFRKDPAIPLVVSEVNPSAIDDALTKPKGIIANPNCTTMAAMPVVSGICVRVPVFTGHKGAALNTVQIAELVAARLG",
    "response#2": "MTKTGIIGWRGMVGSVLMERMRTENDFQRFEPLFFSTSQAGQAGPEINGTTYELLDAFNVELLQGMDIILSCQGGDYTKEVYPKLREAGWQGYWIDAASTLRLEKDAIIVLDPVNRAVIDAALASGKKDFIGGNCAVSLMLMALKPLHDEAGLVRLIVSTYQAVSGGGVAGVDELDKQIRETAGRATELTHDGSAVPFPDPVLYARPIAFNVVPYAGSLLEDGSGETEEERKLRNESRKILGIADLLVSGTCVRVPVFTGHSLSLNAEFSRPLSVARATELLADAAGVELTDIPTPLQAAGKDASFVGRIRVDPGVPDGRGLALFVSNDNLRWGAAEPLRRMLNLAVVGATGAVGRIVLEQLALRKLPINRLKLLASQRSVGKEVRFADETLKVQLLEPDAFQGIDIVVASTPDEVSAEFASWATDAGAVVVDESGYWRMDPKVPLVIPEVNPEAIHDLAPIHRAVGIERINVATYQSVSGAGRSGGEELGRQTAALLNFQDPEPSKFPVQIAFNLIPHIDDFQDNGYTKEEMKMVWETRKILEDASIQVNPTAVRVPVFFGHAEAVHIETRERISADAARRLLQAAPGVVVVDERKPGGYPTPAGHAAGKDPVFVGRIREDISHPRGLDLWVVADNIRLAPIHRAVGIERINVATYQSVSGAGRSGGEELGRQTAALLNFQDPEPSKFPVQIAFNLIPHIDDFQDNGYTKEEMKMVWETRKILEDASIQVNPTAVRVPVFFGHAEAVHIETRERISADAARRLLQAAPGVVVVDERKPGGYPTPAGHAAGKDPVFVGRIREDISHPRGLDLWVVADNIR",
    "response#3": "MAKTGIIGWRGMVGSVLMERMRTENDFQRFEPLFFSTSQAGQAGPEINGTTYELLDAFNVELLQGMDIILSCQGGDYTKEVYPKLREAGWQGYWIDAASTLRLEKDAIIVLDPVNRAVIDQALARGAKDFIGVSGICVRVPVFTGHLAPIHRAVGIERINVATYQSVSGAGRSGGEELGRQTAALLNFQDPEPSKFPVQIAFNLIPHIDDFQDNGYTKEEMKMVWETRKILEDASIQVNPTAVRVPVFFGHAEAVHIETRERISADAARRLLQAAPGVVVVDERKPGGYPTPAGHAAGKDPVFVGRIREDISHPRGLDLWVVADNIRELRGHSAAELELAPIHRAVGIERINVATYQSVSGAGRSGGEELGRQTAALLNFQDPEPSKFPVQIAFNLIPHIDDFQDNGYTKEEMKMVWETRKILEDASIQVNPTAVRVPVFFGHAEAVHIETRERISADAARRLLQAAPGVVVVDERKPGGYPTPAGHAAGKDPVFVGRIREDISHPRGLDLWVVADNIRLAPIHRAVGIERINVATYQSVSGAGRSGGEELGRQTAALLNFQDPEPSKFPVQIAFNLIPHIDDFQDNGYTKEEMKMVWETRKILEDASIQVNPTAVRVPVFFGHAEAVHIETRERISADAARRLLQAAPGVVVVDERKPGGYPTPAGHAAGKDPVFVGRIREDISHPRGLDLWVVADNIRLAPIHRAVGIERINVATYQSVSGAGRSGGEELGRQTAALLNFQDPEPSKFPVQIAFNLIPHIDDFQDNGYTKEEMKMVWETRKILEDASIQVNPTAVRVPVFFGHAEAVHIETRERISADAARRLLQAAPGVVVVDERKPGGYPTPAGHAAGKDPVFVGRIREDISHPRGLDLWVVADNIR"
  },
  {
    "reference": "MPMSLLKMFPQARRDALIRELTLDSRALRPGDLFLAVPGTRSDGRAYIADAIAREAAAVAYEAEGAPPLAEAAATVLVPVKGLQAQLSAIAGRFYGDPSRSLELVGVTGTNGKTSVSQLVAQALDLLGTRCGLIGTLGIGFYDDLQCGRHTTPDPLAVQAELARLKQAGARAVAMEVSSHGLDQGRVEALDFDVAVFTNLSRDHLDYHGDMAAYGEAKARLFAWPGLCCRVINLDDPFGRELAAREAPSRLIGYSLDDPAATLYCAEAEFGEDGVRARLVTAQGDGLLVSPLLGRFNLSNLLAVVGALMGLGHPLDEILRVLPQLQGPAGRMQRLGGGTQPLVVVDYAHTPDALEKVLDALRPHARGRLLCLFGCGGERDRGKRPLMAAVAESRADAVWVTDDNPRGEPAERILADIRAGFANPAAVTFMPGRAAAIRALIAAAAPEDVVLLAGKGHEDYQEIAGVRQPFSDIEQAARALAAREVPDA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.2. The protein design must feature the Meso-diaminopimelate recognition motif motif.3. The ATP, meso-2,6-diaminoheptanedioate binding site should be located in a region of the protein that is accessible to the ligand.4. The designed protein should contain one or more Mur ligase central, Mur ligase N-terminal catalytic, Mur ligase C-terminal domains that are essential for its function.5. The protein should be optimized for activity in Cell wall biogenesis, peptidoglycan biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.6. The ideal protein would be one that plays a critical role in cell cycle, peptidoglycan biosynthetic process, cell wall organization through magnesium ion binding, ATP binding within cytoplasm. The designed protein sequence is ",
    "response#1": "MTVITGLAVDSRAVRPGFLFAALPGSRVHGAAYIPTALAQGAAAVLTDAEGAAQAAAALADSPAALIVACDPRQALSLAAGRFHGRPSRDLKVVGVTGTNGKTTVCTLVYQVLRKLGYNAGLLGTVETRIGDRVTESLLTTPDAVSLAEMLEQMAAIDTAFAVMEVSSHALEQQRVGGIRFDVAAFTNISQDHLDYHRDMASYIYAKKRLFTMLDDKAVAVVNRDDPASFRITEGCRADIWEFSFREDRDFRILDEGPDGLVLDLDGAIVSTPLTGRYNAYNVAQAYGVARLLGLETGDIARALGQARGAPGRMQLAATRANGAAVYVDYAHTPDAIATALKALRPHVMGRILIVFGAGGDRDRSKRPLMGAAARDHADVLFVTDDNPRSEDPAAIRAEIMQACPEVNEVGDRAEAILRGVDALLPGDALLIAGKGHEAYQEIRGEKLPFDDRAVARELLAGL",
    "response#2": "VTGTNGKTSVATFARQIWVALGHAAINIGTTGIEGAWAAPSSHTTPDPITLHSMLAQAARAGVTHAAMEASSHGLDQRRLDGVRLKAAGFTNFTQDHLDYHGTFEAYFAAKAGLFDRVLPPDAAAVINMNDPKGADLRALASAKGQRIITVGHDAGNDLCIAGQRFDATGQEVRYLWQGAPHLVRLNLVGGFQAENVALAAGLGIALGLDPAAVEKALSSFGGVKGRFETVDQGQPFSVIVDYAHTPDGLENVLMTAKKVARKRLITVVGCGGDRDRTKRPKMGSVAAQWSDWVVITSDNPRTEKPEQIIEDILPGIHGVTETSYEVIVDRKSAIKRALMLAEADDLILIAGKGHETGQIVGDRVLPFSDHEEVRKALA",
    "response#3": "VTGTNGKTTTTFLIEHLAAKAGLSTALFGTLANRWPGCNITATHTTPFADQLQAQLAAALATGVQLAAMEVSSHALHQQRVAGCNFSGAVFTNLSQDHLDYHPSLEAYYEAKAMLFEPIYLKGMAVINSDDPHGKRLAAHLAAKCWRSSLLDPTAELFMDALQFSAAGVAGVIHSPKGAAAFQSPLVGRFNLMNLLQAVGVTGTNGKTSCAWWLAQALSNAGMPCGVIGTLGTGRPPQVEFFGLTTPDPVLLQRQFRRFADEGLKACAIEASSIGIVERRLDGTRIRVGIFTNFTQDHLDYHGTMEAYWDAKIELFGWAGLQAAVVNIDDEKGHALAASLSGQGLDVWTVSCREPARLAAQGIGYGDAGLRFEVCEGNERRALATSVIGQYNVSNLLGVIGALLGLDYPLDEILRVLGEFGGVGGRVEKVDRGQAFLAVVDYAHKPAALEAVIATLRSQIDGRLAVVVGAGGDRDTGKRPLMGAAAVRGAELVVITDDNPRTERPDDIRAQVLAGAHEVAEAERPAGAAPVREIGDRREAIRAAVAWAEPGDVVLIAGKGHETYQEIDGVRHPFDDAEVVREALASKG"
  },
  {
    "reference": "MRGKTIVVGVSGGIAAYKACEAVRLLVQAGATVQVVLTRAAEEFVTPLTLQALSGRPVARRTFDLTEESEIGHIRIADTADALLIAPATANVIAKLATGIADDLLTTVVLATRAPVLVAPAMNVHMWENATVQANVARLRDAGHVVIEPASGALACGYEGAGRLPEPDVLVEEVAVAVTEKTLAGRRVLVTAGPTWEALDPVRHVANRSSGKMGFAVARAARRRGARVTLVTGPTALADLRGVECVHVTSAREMEKAALHHAAAADCVVMAAAVGDYRPLEAARHKIKRGRGDLEIRLTPNPDILAKIGALPGQRVIVGFAAETRDLVANARRKLQAKGVHLMVANDVTAPDAGFDVDTNIVTLIDRSGRVERFEKMSKDEVAGVILDRVRDLRTPRTTVRAAPRRLRRR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein contains novel DNA/pantothenate metabolism flavoprotein C-terminal domains that confer a unique function or activity.2. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.3. The protein should be designed to catalyze the following reaction H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. The protein should be able to bind CTP ligand in a variety of conditions5. The protein should be optimized for activity in Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.6. The protein design must be able to perform FMN binding in order to contribute to pantothenate catabolic process, coenzyme A biosynthetic process.7. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A. The designed protein sequence is ",
    "response#1": "MNVLIGVTGGVAAYKVCEVVSTLAKSGASVRVMMTARSQDFVSALTFAALARNAVYTDVEFWSAQQGRPLHIELGEWADVLAITPLTANTLGKLAHGLADNLLTNTVLASVCPVLLAPAMNTDMWQQQSVQRNWEGLRQDARYHTVGPGAGRLACDRIGTGRMAEPVEIVAQIDAVLHHGGPLTGKHVVVSAGPTREPVDAVRVLTNRSSGRMGYAIAAAAWRRGARVTLISGPTEIAPPAGVGVVRAETAADMERAVRAALPEADALVMAAAVSDFRPAAPHAGKLKKEQAPDAIPLEASPDVLRVTRDARPSHLVAVGFALESSDGVANARAKLESKTLDLIVLNPAGEEGAGFETDTNRVTLLSRTGDIEDLPLLPKDEVADRILDRVAALLA",
    "response#2": "KKLLLGVTGGVAAYKVAELARLLTQKGVVVQTVMTKSACRFVGPATFQSLTGLPVFTELWEANVASNMAHINLSRNVEMILIAPASADFIAKLACGLADDLLSTLCLARNCPMMIAPAMNRQMWENPATQRNINRLQDDGVIVAGPADGEQACGEVGPGRMLETDQVLAATETILLTGKRLLISTGSTQEYIDPVRYIGNPATGRMGIALALAGLHRGASVTLVHGPMSAELKATVPGDVRTIGVTTAAEMEQVLLAETAIADWIIMAAAVADVRPTTTADFKLPKKDLPSSLPLSAVPDIVAKLSAGRTAGQKVIGFAAQTGDVVAPALDKLKRKQLDVILANPIDQPGSGFGSLDNEGVLEGLSVLVTAGPTREAADPVRFISNASSGKMGYEIARAAVEVGASATLVSGPTALNKPANVHCIDVVSAREMHDAVMAHIDGKDIFIAAAAVSDYRPIDYVPTKRKKKAMNTTLALEPTPDILAEVTSMPNHPFAVGFAAETDELEYNARQKYQRKSLDMIAANRVGIPDSGFESDQNARLKLLKGGQEVEVPVASKAEVAHRVLDVVLDRLG",
    "response#3": "MNVLIGVTGGVAAYKVCEVVSTLAKSGASVRVMMTARSQDFVSALTFAALARNAVYTDVEFWSAQQGRPLHIELGEWADVLAITPLTANTLGKLAHGLADNLLTNTVLASVCPVLLAPAMNTDMWQQQSVQRNWEGLRQDARYHTVGPGAGRLACDRIGTGRMAEPVEIVAQIDGALGRSGPLAGVKTLVTAGPTHEALDPVRYIANHSSGKQGYAIAKAMQQLGADVTLVSGPTKLSPPQGVNFISITSAEEMLAACQNAMPSDIAICAAAVADWRVAEPSKQKIKKKNNTEAPTLSFIQNPDILATLSQSGPNRPGLVIGFAAETEHVTENAVSKRERKGCDWILANDVSPATGTFGGDENTLFLVTASGVEDWPRMGKDEVARRLAVRIAQALD"
  },
  {
    "reference": "MKIPMILGATAVGKTQFLVKLASLIPIEVVSVDSRQIYRYMDIGTAKPTREELSKLKHHVIDVVDPDEDFNVFEYRKIALKAMDEIVKKGRIPVFAGGSGLYAETLMKGIVENVPRNDKVREALKTLEVNAPGSLRKLLEKVDHEAYEKIHPNDLKRTIRYLEVFFTTGKTLTSLQKIHKCSNQFTVIILERDRRELAERIENRVDKMVESGLIEEVVRLKEMGYTRELNSQQTIGYAEIWSYLEGEVTLERATELIKRNTRRFARRQIIWFRRYKDAKRISLSETNENDILSLLKEDILNVWGGKYG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have ATP binding to facilitate tRNA processing.2. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.3. The protein must exhibit the following characteristics: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)..4. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate, ATP binding.5. The desired protein should be able to perform adenosine(37) in tRNA + dimethylallyl diphosphate =   diphosphate + N(6)-dimethylallyladenosine(37) in tRNA The designed protein sequence is ",
    "response#1": "MNGDALLSVDDLSVRFGSNRGVVEAVRGVSLTVREGETLGLVGESGSGKSTTGKAIMGLLRPTGGKVTFHGRDLATMSRREMNALRRDLQIVFQDPYASLNPRLSVGQAIAEPLRVHGIGEGPARRERVLELLEQVGLSPQFAGRYPHELSGGQRQRVGIARALAARPSVMVCDEPTSALDVSAQAGILNLLADLQRELGLTYLFISHDLSVVRYISDRVAVMYLGEIVEVAPTSELFQRPQHPYTRALMAAVPIPDPRAKRQRIVLEGDVPSPLNPPSGCVFRTRCPDAMPECRKTIPALRDVAPGHFAACHLYA",
    "response#2": "MKNIIIATGNKGKLKEISELLSPLGYEVKPQSDFDLPEVEETGTTFVENAIIKARHAAKITGLPAIADDSGLAVDALNGAPGIYSARYAGENATDADNNQKLLKELEGTPWDRRAYYVCALVYMRSAKDPVPIICEGVWEGEILTEKRGKGGFGYDPLFLVDDLGKTAAELPPEEKNRISHRGQALRKMIELLKERL",
    "response#3": "MKKIVFATGNAGKLREFQHILGGLPVELLSLNEIGCTEDIPETAPTFEGNALLKARYVKDHYGLDCVADDSGLEVDALGGAPGVYSARYAGEGASYSDNVQKLLHELKGQTDRSARFRTVIALYLNGEQHFFRGVVEGMIAEEKQGEKGFGYDPVFRPAGYGKTFAELSMEEKSRYSHRGKALRAMIAFLKEEA"
  },
  {
    "reference": "MSENSSHSPAPGHNAASVRGPASGHNAESVRGPASGHGVASGNRVVLASNNAGKLREFAAILGQAGLAMVAQGELGVGEAEEPYATFVENALAKARHAARHTGLAALADDSGLCVDALDGAPGVYSARYATLHGGEKSDAANNACLIRNLAGAVDRSASYVAVLVYLRSADDPRPLIAQGVWRGSVLEAPRGSNGFGYDPYFYLPELGHSAAELDPAEKNRVSHRARALARLLQALREE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.2. The designed protein should have a binding site that is specific to the target substrate ligand.3. The protein must be able to nucleotide binding, ITP diphosphatase activity, dITP diphosphatase activity, XTP diphosphatase activity.4. The protein should be designed to have high stability and activity towards dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MKAILFATRNKGKITEFQ",
    "response#2": "MKILVLIL",
    "response#3": "MNEVKLVFATNNAHKVSEV"
  },
  {
    "reference": "MPRYLAIVAYNGAPFRGWAKQPGYHTVQGQIEQNLALIYQTPINIYGSGRTDKGVHAINQTFHVDLPDKLPLKQLIAKLNQLNYPDIFVKALVPVAPRFHARFAVKTKVYEYLINTRAFNPAQYHTVWQYNHPLDLAQLEADTTLFLGRKDFWSFSTASQKDTQRTISKIIVQQDHTGTVKLTFFGNGFLRNQIRMMVASLVALNNRTLMRETVQQLFDQPQKGGCKVKAPACGLYLKTVLYE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein is the optimization of the Nucleophile to achieve high catalytic efficiency for uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA.2. The designed protein should have tRNA pseudouridine synthase activity to facilitate tRNA pseudouridine synthesis.3. The substrate binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "AMEPLLGKHHLAAFHRAGSARRHSWVEVQGVECYRRGPMVYLEIQANGFLYGMVRLLVGMLAEVGSGQRSLTDFNDIWVNQRRDLVKYAAPAKGLCLLRVGYA",
    "response#2": "AAQCILGENDFTSFRAVQCQSKTPWRNVMHINVNRYGHYVVIDIKANAFVHHMVRNIAGSLMEIGCGNQDINWMAQLLALKDRAKAAATAKAAGLYLVAVDYA",
    "response#3": "AMEPLLGKHHLAAFHRAGSARRHSWVEVQGVECYRRGPMVYLEIQANGFLYGMVRLLVGMLAEVGSGQRSLTDFNDIWVNQRRDLVKYAAPAKGLCLLRVGYR"
  },
  {
    "reference": "MSDSFGCVVLAAGKGTRLKLKDPKPLVPLMGRRLVDYSIESLKKFMDANSSKGAVSLVIGHEKEKVQAHAEESAKKFDVQVSFSIQEKQLGTAHALKCYFDQNAEAANYKYTVIMCADTPLISSEVLVELFAALKKDDLDGVAATFETLDPTGLGRIIRSEKEGFKITEHKDANEEELLIKEVNSGMYIVKTSYILEHLSNIKSENAAGEFYLTDLFQEDSKVKPCLFDDENTFLGVNDLEQLERIEVLLRRRKKIELRNAGVRFIDSRHVYIDEDVQIGEGSTLYPNATLYGNSVIGKDCVLETGVIIKNSEVQDGTTIKAYSSLEESFVGKEAAIGPYARLRPKANIGSYSKIGNFVEIKKSNLKEGVKVSHLSYVGDAEIGEETNIGCGFITCNYDGANKHKTIIGKGCFIGSDSQMIAPVTLGDECYVASGSTINQDIPSGGFGIARGRQSTKEGMAKRFIKKK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.2. The protein design should aim to mimic or enhance the function of natural proteins involved in UDP-N-acetylglucosamine biosynthetic process, lipid A biosynthetic process, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "LVLAAGLGTRMRSNLAKVLHKIDGRPLVNHVCRMATALAPRKIYVVIGHQGEDVKRAVLEELDESHSAFVWQKEQLGTGHAVNSAREFFENEDSTLLILSGDVPMIKFETLAALIQQHHAHRGRGAACTILTVKLDDPTGYGRIVRDEAGLFEKIVEQKDASDEERAVREINSGIYCFDTKKLFAALSSVENKNAQNEYYLTDVPSLVTPYRDQGRG",
    "response#2": "LVLAAGLGTRMRSNLAKVLHKIDGRPLVNHVCRMATALAPRKIYVVIGHQGEDVKRAVLEELDESHSAFVWQKEQLGTGHAVNSAREFFENEDSTLLILSGDVPMIKFETLAALIQQHHAHRGRGAACTILTVKLDDPTGYGRIVRDEAGLFEKIVEQKDASDEERAVREINSGIYCFDTKKLFAALSSVENKNAQNEYYLTDVPSLVTHAEDVMGVNSRL",
    "response#3": "MVDTQVADGASVSNAVVVESVIGPGATVGPYSYMRPGTVLQEKAKVGTFVEIKKSTIGKGSKVPHQTYLK"
  },
  {
    "reference": "MKPLEIDVFLCRSDNFGVLLHDPESGATAAIDAPEEGPILRALDGHGWKLTHIFTTHHHQDHVEANLVLKDKFRCEVHGPYDEAIAIPGLDRSQADGDEFEFAGRRVQVIATPGHTAGHICYYLPDDGLLFAADTLFAMGCGRLFERPAADMWHSFQKLMALPDDTRVYFGHEYTLSNARFALTVDPDNAVLRERAARVETARQANEFTIPTTIGLEKQTNPYMRVADAGIRRHLGLEGAADADVFAEIRTRKDNF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl- glutathione to form glutathione and D-lactic acid..2. Ideal protein should have a binding affinity for key enzymes in the methylglyoxal degradation, Secondary metabolite metabolism, (R)-lactate from methylglyoxal: step 2/2 pathway to enhance the overall efficiency of the pathway.3. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation.4. The protein design should be able to enhance the efficiency of methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione.5. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MDYLDGNLRFALALEPGNPALISRQGRLSEMRREGRLPMPWTLAEELATNPFLRAPLPQMKAAVGLPQASDTVVFAEIRARKDLA",
    "response#2": "IPAFQDNYIWVLTNDEGRCVIVDPGEAAPVLKAIAEHKWMPEAIFLTHHHHDHVGGVKELLQHFPQMTVYGPAETQDKGATHLVGDGDTIRVLGEKFTLFATPGHTLGHVCYFSHPYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTLICCAHEYTLANLHFAKAVEANNPALIEYTVKAHALRAENKPTLPSSIALEKAINPFLRLDSLEIQKSLAVQFQQPIADPVQSFALLRQWKDNFN",
    "response#3": "MLKGEYTLSNAAFALHVDPTNTALQKRRQQANAMRLAGLPTLPISLESELNTNPFLRTAAPTIHAATATHLQRTPIDEVEVFATLRHWKNNEYTLANLHFAKAVEANNPALIEYTVKAHALRAENKPTLPSSIALEKAINPFLRLDSLEIQKSLAVQFQQPIADPVQSFALLRQWKDNW"
  },
  {
    "reference": "MKEQAAIQRSSAKCSNAGSACKATVIGGPVKNPSPVCLGLQQAVCLVVPLPDCFCAHQLLGSSCRQHEQGLPRGKGIDEGDGEKAEGTALLIPATSLVLYTFYVTGTGLGSQKKGGGEGGGGGGSGKLEAAGGGSREIWCCGRVGEAAGVGSAPGRAPGVPELRSRPGETRRRLHGDCALPAGWSRIDWYRITCLCGIPNQVNIEICSFYFSWTSPILKKGYRQRLELLDIYQISSADSADNLSEKLEREWDRELASKNNPKLIHALRRCFFWRFTFYGILLYLGEVTKAVQPLLLGRIIASYDPDNKAERSIAIYLAIGLCLLFIVRTLLLHPAIFGLHHIGMQMRIAMFSLIYKKMKSILLNLYFPLTNFDSKYLTELKLTRKAAYVRYFNSSAFFFSGLFVVILSVLPYAFIKGIILRKIFTTISFCIVLRMAVTRQFPGAVQTWYDSLGAINKIQDFLQKQEYKTLEYNLTTTEVVMENVTAFWEEGFGELFEKAKLNNNNRKISNGDSKLFFSNFSLLGTPVLKNINFKIEKGQLLAVAGSTGAGKTSLLMMILGELEPSEGKIKHSGRISFCSQFSWIMPGTIKENIVFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGHLDVLTEKQIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLRPDFSSKLMGFDSFDQFSAERRNSILTETLRRFSLEGDVSASWNE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein contains novel ABC transmembrane type-1 domains that confer a unique function or activity.2. The protein must exhibit ABC-type transporter activity.3. For general function, the protein need meet that The ion channel is also permeable to HCO(3-); selectivity depends on the extracellular chloride concentration; Epithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis4. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "TTEVVMENVTAFWDEGFGELFENAKQNNKNRNISNSDNSLFFSNFSLLGTPVLKDINFQIERGQLLAIAGSTGAGKTSLLMMLMGELEPSEGKIKHSGRISFCSQFSWIMPGTIKENIIFGICYDEYRYRSVIKACQLEEDISKFAEKDNVVLGEGGLTLSGGQRARICLARAVYKDADLYLLDSPFGHLDVLTEKQIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLRPDFHSKLMGYDSFDQFSAERRNSILTETLRRFR",
    "response#2": "TTGLELDKITAFWDEGSGEIFTKTKQEHGNNKIPSTNDGLFFSNFTLHFTPVLKDINFKIEKGKLLAVAGSTGAGKTSLLMLIMGELEPLEGKIKHSGRISFCPQVSWIMPGTIKENIIFGVSYDEYRYKSVIKACQLEEDISKFPEKDDTVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDMLTEKEIFKSCVCKLIVNKTRILVTSKLEHLKIADKILILHGGCCYFYGTFSELQGQRPNFSSELMGWDAFDHYSPERRNSILTETLRRLR",
    "response#3": "QGFGELFEKAKLNNNNRKISNGDNSLFFSNFSLLGSPVLKDINFKIEKGQLLAVAGSTGAGKTSLLMMILGELEPSEGKIKHSGRISFCSQFSWIMPGTIKDNIVFGVSYDEYRYKSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKQIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLRPDFSSKLMGYDSFDQFSAERRNSILTETLRRFR"
  },
  {
    "reference": "NALFILGNIGNNDVNYAFPDRAIEEIRFYVPFITEAVANATREIIR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should be able to enhance the efficiency of alkaloid metabolic process. The designed protein sequence is ",
    "response#1": "LWDMYFINLVERQNIWYRRVIQGKSKPWTDDPVMSTYHFTNVDRRLDRVTLHYIDKVLCNLEDSYESKKFLLLNTFIYRLFVRPETTDAMGYIFPETFEEDWERAKAALRARRESGEPVFTDAYFVNDLKSANPDRANSSNKTENAIHLIQFIIDHLDELAEFTFNPKNSMEEVIEKFTMIPAVGNFNAYEVALDLGIVKEMTGIDFVDWTPDHYANVGPGCKKGIEYVFEDLGNMSHLDIVFFITSVYKGELERLGLEYKYQEGCKELDLRALEGWCCEMSKYFR",
    "response#2": "LWDMYFINLVERQNIWYRRVIQGKSKPWTDDPVMSTYHFTNVDRRLDRVTLHYIDKVLCNLEDSYESKKFLLLNTFIYRLFVRPETTDAMGYIFPETFEEDWERAKAALRARRESGEPVFTDAYFVNDLKSANPDRANSSNKTENAIHLIQFIIDHLDELAEFTFNPKNSMEEVIEKFTMIPAVGNFNAYEVALDLGIVKEMTGIDFVDWTPDHYANVGPGCKKGIEYVFEDLGNMSHLDIVFFITSVYKGELERLGLEYKYQEGCKELDLRALEGWCCEMSKYFR",
    "response#3": "LWDMYFINLVERQNIWYRRVIQGKSKPWTDDPVMSTYHFTNVDRRLDRVTLHYIDKVLCNLEDSYESKKFLLLNTFIYRLFVRPETTDAMGYIFPETFEEDWERAKAALRARRESGEPVFTDAYFVNDLKSANPDRANSSNKTENAIHLIQFIIDHLDELAEFTFNPKNSMEEVIEKFTMIPAVGNFNAYEVALDLGIVKEMTGIDFVDWTPDHYANVGPGCKKGIEYVFEDLGNMSHLDIVFFITSVYKGELERLGLEYKYQEGCKELDLRALEGWCCEMSKYFS"
  },
  {
    "reference": "MLGIGRGAAPGPQQQQHLPGQPAGAAAPVPPAHRFLQPIGQCDAALGDLLSELQRDPWPVPQGKRYLRSTGAALSIAVGLLECTYPNTGGRIMTFVGGPCSQGPGQVVDDELKHPIRSHHDIHKDNVRFMKKAIKHYDALALRAATNGHSVDIYSCALDQTGLLEMKQLCNSTGGHMVMGDSFNSSLFKQTFQRVFARDGRNDLKMAFNATLEVKCSRELKISGGIGSCVSLNVKSPSVSDVKIGMGNTVQWKLCTLNPSSTVAYFFEVVNQHAAPIPQGGRGCIQFITQYQHPSGQRRIRVTTLARKYVLA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER)2. The designed protein should be involved in positive regulation of cuticle pigmentation, regulation of COPII vesicle coating, protein localization to plasma membrane by localizing to Golgi stack and exhibiting metal ion binding. The designed protein sequence is ",
    "response#1": "GFNAVLEVLTTKELKVTGLIGHAVSLNKKSTSVGETECGIGNTCTWKMCGIDPSSSYGIYFEIANQGGPSQNMQSPQKGMMQFLTYYQHSSGHFHLRVTTIARNC",
    "response#2": "GFNAVLEVLTTKELKVTGLIGHAVSLNKKSTSVGETECGIGNTCTWKMCGIDPSSSYGIYFEIANQGGPSQNMQSPQKGMMQFLTYYQHSSGHFHLRVTTIARNC",
    "response#3": "GFNAVLEVLTTKELKVTGLIGHAVSLNKKSTSVGETECGIGNTCTWKMCGIDPSSSYGIYFEIANQGGPSQNMQSPQKGMMQFLTYYQHSSGHFHLRVTTIARNC"
  },
  {
    "reference": "MLDGRRVLLIISGGIAAYKSLELIRRLKDRGCAVRCILTKGGANFVTPLAVAALSGDKVYQDTFDLKDESEMGHIRLSREADLVVVAPATANLLAKMAAGIADDLASTALLATDKPVLVAPAMNTLMWEHPATRANMATLEGRGVARIGPNAGDLACGEVGGGRMAEPAEILAAIETSLSDGPLKGVRAVVTSGPTHEAIDPVRYIANHSSGKQGHAIAAALARLGAEVTLVSGPVHIPDPAGVRVVRVDSAVEMLNGVRAALPADVVVCAAAVADWTVANRATEKRKKKVGAPPPVIELAPNPDILMTVSKAEGGLRPRLVVGFAAETEALLDNARDKLARKGCDWIVANDVSEGSGTFGGDANLVHVIDADGIDSWPRMGKDAVAARLAERIANVLRGL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must have high specificity towards metal ion binding, FMN binding, phosphopantothenate--cysteine ligase activity.2. The designed protein should interact with enzymes and substrates within the Cofactor biosynthesis pathway to enhance or regulate its activity.3. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.4. The protein requires Mg(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "RVTLGVSAGISIYKACDLVRRLMDRNCEVQVVLTSHAARMVAPVTFQALSGRPVITDLFEGAGEPSIAHVELARWEELLVVAPATANVLGKFARGIADDFLTTHYLANRGAPVLLAPAMNGAMWRHPAVQENLRILEQRGHAVLQPGKGSLACGDVDEGRLPEPAAIAQEAVEAILAAV",
    "response#2": "RVTLGVSAGISIYKACDLVRRLMDRNCEVQVVLTSHAARMVAPVTFQALSGRPVITDLFEGAGEPSIAHVELARWEELLVVAPATANVLGKFARGIADDFLTTHYLANRGAPVLLAPAMNGAMWRHPAVQENLRILEQRGHAVLQPGKGSLACGDVDEGRLPEPAAIAQEAVLARL",
    "response#3": "MRILLGITGGIAAYKAAGLLRALSELGHEVTALPTENALRFIGDATLEALSGKNIDPDLYGDVASVRHVQLGQEADLIVVAPATAAFLGRYASGIADDLLLNALLATHAPVVVVPAMHTEMWQNAATVENVETLRARGVRVMEPASGRLTGEDSGPGRLPEVEDIVSFLAAG"
  },
  {
    "reference": "MANIKSQKKRVRTNEKAHQRNVAVKSALKTYIRRTREAIASGDKAAAEAAFAIAGRKLDQAAGKGVIAKNQAANRKSSLALQINAM",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Binds directly to 16S ribosomal RNA.. The designed protein sequence is ",
    "response#1": "MPIGVPKVPFRSPGESDA",
    "response#2": "MPSGGMK",
    "response#3": "ERGPA"
  },
  {
    "reference": "MFFWLMCYLVDVWLISASDVGHCPDPLLVTDEFSSLEPVNVNDTFMFKCNEHCIFKGSNWSQCRENHTRVTHSPVSKSRDCGPPETPTHGYFEGRDFKSGSTITYYCEARYRLVGTQHQQCIDGEWTSAPPICELIQEAPKPAELELEKAFLAFQESKELCKAIKKFTQRLKKSDLTMEKVKYSLERKKAKLKAKMLL",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b; Controls the classical pathway of complement activation.2. Include a signal peptide as an essential component of the protein.3. The presence of Sushi 1; atypical; lacks a Cys domains is critical for the interaction of the designed protein with its intended targets.4. The protein should be engineered to have a higher affinity for a specific molecule or receptor in extracellular space that is critical for innate immune response, complement activation, classical pathway, negative regulation of complement activation, classical pathway. The designed protein sequence is ",
    "response#1": "LCGMVWEHKKGNDYHKQPWQAKISVTRPLKGHETCMGAVVSEYFVLTAAHCFMVDDQKHSIKVSVGGQRRDLEIEEVLFHPKYNINGKKAEGIPEFYDYDVALVKLKNKLKYGQTLRPICLPCTEGTTRALRLPQTATCKQHKEQLLPVKDVKALFVSEQGKSLTRKEVYIKNGDKKASCERDATKAQGYEKVKDASEVVTPRFLCTGGVDPYADPNTCKGDSGGPLIVHKRSRFIQVGVISWGVVDVCRDQRRQQLVPSYARDFHINLFQVLPWLKDKLR",
    "response#2": "SAQERTPWHVTIKPKSQETCRGALISDQWVLTAAHCFRNAEDRTLWRVSVGDPNFQGSKEFQIEEAVISPGFNVFSKKSQGIPEFYGDDIALLKLTQKVKMSTHARPICLPCTVGANLALRKLPGSTCRDHEKELLNQVSIPAHFVALNGDKLNINLKTGSEWTNCVKVVLKDKTTFPNLTDVREVVTDQFLCSGTQGDDSPCKGESGGAVFLERRLRFFQVGLVSWGLYNPCGQ",
    "response#3": "IFGGQPAKIENFPWQVFFNHPTAGGALINEYWVLTAAHVVEKNSDPSMYAGITALRLADLENAQRLYTKRVIIHPGWKEDDDLNPRTNFDNDIALVQLKDPVKMGPKFSPICLPGTSSEYNLSPGDMGLISGWGRTEKRLHVINLRGAKVPVTSLETCKQVKEENPTARPEDYVITDNMICAGEKGVDSCKGDSGGAFAFQVPNVKAPKFYVAGLVSWGKKCGAYGVYTKVKNYVDWILKQ"
  },
  {
    "reference": "MKNLNLYHFFDCKDRKITMYRLTEKGERIRDMFSDIAPRYDFLNRLLSFGVDRRWRRNAVKCIRWSEGGRVLDVATGTGDVALEIARQTPPSVAIVGVDFSEGMVALGRDKVAGSPYAGRITMEIAPCEAIPFPDDTFDSVTIAFGIRNVVDRSQGLSEMLRVLKPGGRAVILEFSTPRSRLFKRIYSFYFLRVLPVIGGLFSQFGAYKYLPDSVLEFPSQEEFKALMASVGFRDTAHRDQTFGIATIYTGEKGLK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must be optimized for demethylmenaquinone methyltransferase activity. The designed protein sequence is ",
    "response#1": "LEVGCGGGLLTEHLARLGAQVAGIDLGEKLIEAAREHLKCSSPELASNVVYKIEPVDQHAKANCECYDAVIVSEVLEHVKDKVSLLEASVRSLKPGGSIFG",
    "response#2": "LEVGCGGGLLTEHLARLGAQVAGIDLGEKLIEAAREHLKCSSPELASNVVYKIEPVDQHAKANCECYDAVIVSEVLEHVKDKVSLLEASVRSLKPGGSIFG",
    "response#3": "LEVGCGGGLLTEHLARLGAQVAGIDLGEKLIEAAREHLKCSSPELASNVVYKIEPVDQHAKANCECYDAVIVSEVLEHVKDKVSLLEASVRSLKPGGSIFG"
  },
  {
    "reference": "MATTVVILAAGQGKRMRSSLPKVLHPLAGRPLLEHVLAAAGALKPREIRVVHGHGGERVKEAFGSRALTWVHQAEQRGTGHAVRQALARIPARDVVLVLYGDVPLIQPETLRRLVARARAGQLAVLTAEFADPAGYGRIIRSGGKRGGIVAIVEDRDATPAQRAIHEINTGLLACPAAALSRMVRRLRADNAQKEYYLTDIIALAVRDGMGVAAVAAGSAAEVMGVNDKAQLAEAGRHLRQRITGELLQAGVTLIDPERIDIHGSLRCGRDVTIYPNVLFEGDVSLGDGVVVEPFSRIRNARLGAGTVVHSHCVIEAGDIGRDCEIGPYARVRPGVVLAGDVKLGNFVEVKKSAIGLHSKVNHLTYIGDATIGERVNVGAGTITCNYDGANKHQTVIGDRAFIGSGVELVAPVTIGAGATIGAGSTISKDAPPGELTVARSKQISIPGWKRPVKTPK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that can modulate the overall flux of metabolites through the UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway, enhancing or suppressing its activity as needed.2. The Mg(2+), UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein should contain one or more MobA-like NTP transferase domains that are essential for its function.4. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc)5. The designed protein should have magnesium ion binding, glucosamine-1-phosphate N-acetyltransferase activity, UDP-N-acetylglucosamine diphosphorylase activity to facilitate peptidoglycan biosynthetic process, cell morphogenesis, regulation of cell shape, lipid A biosynthetic process.6. We need a protein that can perform acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate with high efficiency.7. The presence of Mg(2+) is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "MVILAAGKGTRMYSHKPKVLHALAGKPLVQHVLDCAATLRPQRVCVVYGHGGEMVPQAMAQYDAQFVIQEPQLGTGHAVQQAMPHLPDEGRTLVLYGDVPLIQHSTLHQMQQADPAQLALLTVNLDNPSGYGRIVRDDTGAVTAIVEHKDASDEQRQIREVNTGILAAPARLLKDWLTRLDNNNAQGEYYLTDIVAMAVAEGVPVVTAQPAAAWETLGVNSKVQLAELERIHQRNEAERLLLARVQLARAESVAQERLRRAAMLGGATLIAPQTVFLSHDTKIGRDVTIEPGVVIGPGVEIADGAVIHAYSHLEGALVGERATVGPYGRLRPGARLAAQAKVGNFVEIKGADIGEGAKVNHLTYIGDASVGAHSNIGAGVITCNYDGFFKYRTIIGENAFIGSNSALVAPVTIGDGAYVGSGSVVTKDVSADALAVARGRQRPV",
    "response#2": "MTVIILAAGQGTRMKSSLPKVLHSVAGRPLLEHVVRAAQVLEPLAIHVVVGHGAEQVQAALADYPIRWVLQREQLGTGHAVLQAIPAVAESSTVLVLYGDVPLIGADTLRKLADKAGSAPGFGAFRIDPGGRILRIVEERDATPDERAITLCNAGMMAIDGARALEWLDQVGNANAKGEYYLTDVIGLARAQGGRVASVEGVSIADINRLDIRGSLSCGEDVCIDVNVVFEGDVKLGDGVSIGPNCVLLNVRVGDGVSIHAMSHLEDADIGNDCSVGPYARLRPGTNMAKGARVGNFVETKKSNIGAGSKVNHLSYIGDCEMGEGVNIGAGTITCNYDGVNKSKTEIGDGSFVGSNSTLVAPVEIGEGGFVGAGSTVTKAVGKDELAVSRARQMEIEGWAARFRQAMKLKKDQR",
    "response#3": "MSAILAAGKGTRLKSKYPKVLHRVGGKPLLSHVIAAATRIVPASEIYVVLGHEAKQVEQAIADSGVQFIEQAEQRGTGHALQVARPMLEKFDTVVVLSGDAPLITSETIQSLLKFHRDKHAAMSLLSAHLPHPHGYGRILRAEVPDATGYGRIVRDAAGRVTRIVEEKDASDAQRAIREINSGVFAFDGALLAEALGKVRTDNSQGEEYLTDILGILRGAGHGAGAHLVDDYREIQGINDRVQFAEARRILNDRLLRAAMLAGATIEDPATTWIDVTVRIGQDVTIHPGTQLKGTTVIADGAEIGPDTTLTNMEVGEEATVVRTHGSDSSIGARANVGPFTFIRPGTILAEEGKLGGFVEAKNAQIGRGSKVPHLTYIGDATVGEYSNIGASSVFVNYDGVNKHHTTIGSHVRTGSDTMFIAPVQIGDGVYSGAGTVIKEDVPAGALVVSGGKQRNIEGWVQRKRPGSAAAKAAETALRRTQKSDKKGEGK"
  },
  {
    "reference": "MNKTRYRLDVAYDGANFCGWAPQPGLRTVGGLILDALRLICSEPPDIVVAARTDAGVHALQQVCHVDLISSPDPVWLLHRLRSLLKSETDLHILSAVKAPVNFHARFSAIGRRYVYRVIDKRSSWYPQNRYFVYRVNAFLQDYRMRRAASGLIGLKDFGAFCKPRRMGSTVRHLRQFEVIRQPDGQIHFFLESDAFCHSMVRNLVGSLIEVGRGALTLQDLFCYTKIAKRTPKIPTLPPHALTLIGIDYPQEHLFECQNRKTRQKRT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with RNA binding, tRNA pseudouridine synthase activity is needed to enhance tRNA pseudouridine synthesis in a specific context.2. The Nucleophile of the protein must be engineered to enable effective catalysis of uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA.3. For general function, the protein need meet that Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs. The designed protein sequence is ",
    "response#1": "AASLTIGLHDFGSFATPNPGGTTIREVKTAYWRRVPITPLVPDEMASHEAYRTPSLESGLVVFTIVADAFARNMVRSLVGSCIKVGSGRKSLEWFAGKMAEPVREGSSGPIAPQGLTLEHIAYR",
    "response#2": "YLLGKHNFTSFRASSCQSKSPIKTLTKLDIIKENEEIKLYFSAPSFLHHMVRNIVGSLVLVGKNIWQAEQMQQVLEVADRKAAGPTAPACGLYFIKADC",
    "response#3": "AASLTIGLHDFGSFATPNPGGTTIREVKTAYWRRVPITPLVPDEMASHEAYRTPSLESGLVVFTIVADAFARNMVRSLVGSCIKVGSGRKSLEWFAGKMAEPVREGSSGPIAPQGLTLEHIAYR"
  },
  {
    "reference": "MLIRNYRGLLGTKLGMTQVWDANNRVVPVTVIKAGPNVVTQVKTPDSDGYSAVQLGYGEIDPRKINKPARGHFATSGVTPRRHLVELRTADAGNYRPGQELTGEVFAEGTVVDVTGTSKGKGFAGVMKRHGFKGLGAGHGVERKHRSPGSVGGCATPGRVFKGLRMAGRMGHARTTTPGLTIHAVDTERGFLLVKGAIPGPDGGLVFVRSAAKRPAPEAIAPAATVGAGEEVSA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must exhibit the following characteristics: One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit..2. The protein design must be able to perform structural constituent of ribosome in order to contribute to translation. The designed protein sequence is ",
    "response#1": "MPRKGNVPKREVLADPIHGSMVVAKLINSVMLDGKKGTAQNIVYGAFDIIKERAEENPVEVFEKAMNNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLRWLVKYTRARGEKGMIEKLAKEIMDAANNTGSSVKKKEDTHKMAEANRAFAHYRW",
    "response#2": "PRKGNVPKREVLADPIHGSMVVAKLINSVMLDGKKGTAQNIVYGAFDIIKERAEENPVEVFEKAMNNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLRWLVKYTRARGEKGMIEKLAKEIMDAANNTGSSVKKKEDTHKMAEANF",
    "response#3": "PRKGNVPKREVLADPIHGSMVVAKLINSVMLDGKKGTAQNIVYGAFDIIKERAEENPVEVFEKAMNNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLRWLVKYTRARGEKGMIEKLAKEIMDAANNTGSSVKKKEDTHKMAEANY"
  },
  {
    "reference": "MDLFKSFGGKNNSKNIAKERLQLLLVHDRADVSPKFLEMIKEDILNVISNYVDIDEAGLNVEITKEKRSDNTYIPALHANIPIKKMKQVIR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be able to modulate cell cycle, regulation of division septum assembly in a way that leads to a desirable outcome.2. For general function, the protein need meet that Prevents the cell division inhibition by proteins MinC and MinD at internal division sites while permitting inhibition at polar sites The designed protein sequence is ",
    "response#1": "LKQPVRSGQSVVFTEGDVTVLGSVGSGAEIVAGGSIHIYGTLRGRAMAGVNGNLDARIYCQKIEAELLAIDGYYQTAENIDSTLRSRPVQAWLDDETLKY",
    "response#2": "LKQPVRSGQSVVFTEGDVTVLGSVGSGAEIVAGGSIHIYGTLRGRAMAGVNGNLDARIYCQKIEAELLAIDGYYQTAENIDSTLRSRPVQAWLDDETLKY",
    "response#3": "LKQPVRSGQSVVFTEGDVTVLGSVGSGAEIVAGGSIHIYGTLRGRAMAGVNGNLDARIYCQKIEAELLAIDGYYQTAENIDSTLRSRPVQAWLDDETLKL"
  },
  {
    "reference": "MLQNHSIAEFDPVLWDAIQNENRRQEEHIELIASENYVTKAVMEAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQANAAVYGALLNAGDTILGMDLAHGGHLTHGAKVSFSGKIYNSVLYGITAEGLIDYEDVRVKALESKPKMIVAGFSAYSQVVDWAKMREIADEVGAYLFVDMAHVAGLIAAGLYPNPLPHAHVVTTTTHKTLAGPRGGLILSACGDEEIYKKLNSSVFPANQGGPLMHVIAAKAVCFKEALQPEFKAYQAQVLKNAKAMVEVFKQRGFEVVSKGTENHLFLVSFVKQGLTGKAADAALGEANITVNKNSVPNDPQKPFITSGIRVGSPSITRRGFNEADASTLAGWMCDVLESIGKDNYDQVIAETRAKVLEICKRLPVYGD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.2. The protein design must be focused on enhancing the efficiency of glycine biosynthetic process from serine. The designed protein sequence is ",
    "response#1": "SETDPDIFSAIQKEFGRQQHEIELIASENIVSQAVLDAAGSVLTNKYAEGYPGKRYYGGCQYVDIVEDIAIDRAKKLFNCEFANVQPNSGSQANQGVFNALAQPGDTILGLSLAAGGHLTHGAPVNQSGKWFKAVHYMVKPDSHLIDMDEVRKLAQEHKPRIIIAGGSAYPRKIDFAAFRAIADEVGAIFLVDMAHFAGLVAAGLIPSPFPHAHVVTTTTHKTLRGPRGGMILTNDADIAKKINSAIFPGIQGGPLMHVIAGKAVAFGEALRPDFKVYIKQVMDNARALGEVLVQNGFALVSGGTDTHLVLVDLRPKKLTGTKAEKALGRANITCNKNGIPFDPEKPMVTSGIRLGSPAGTTRGFGVAEFQEIGRLIAEVLDAVDHPERLAAVRHEVAELTRRFPLYPGLE",
    "response#2": "ERIKNLTKDHHNWMKNSLNLIASENITSRAVREAVASDLSHRYAEGLPGERLYEGCDYIDAIEEETIALSKKLYDAEHVNVQPTSGVVANLASFFALTKPGDLLMSINVPEGGHISHASVSAAGIRGLKISSVPMDDSIMNVDIDKTLSKIREKEPKAIVLGGSLFLFPQPVSEVADVAKEVGAKIIYDAAHVLGLIAGKRFQDPVKEGADIVTGSTHKTFPGPQGGIILCKEEIGRKVDNCVFPGVVSNHHLHHMAALGVATAEMLEFGKDYANQTISNAKALAQALYERGFNVLCEDQGFTESHQVAMDVAKLGDVSKMAKTLQYNNIILNKNLLPWDDVNDSDNPSGIRMGTQELTHRGFKEDEMDQVAEFLAEITQ",
    "response#3": "ERIKNLTKDHHNWMKNSLNLIASENITSRAVREAVASDLSHRYAEGLPGERLYEGCDYIDAIEEETIALSKKLYDAEHVNVQPTSGVVANLASFFALTKPGDLLMSINVPEGGHISHASVSAAGIRGLKISSVPMDDSIMNVDIDKTLSKIREKEPKAIVLGGSLFLFPQPVSEVADVAKEVGAKIIYDAAHVLGLIAGKRFQDPVKEGADIVTGSTHKTFPGPQGGIILCKEEIGRKVDNCVFPGVVSNHHLHHMAALGVATAEMLEFGKDYANQTISNAKALAQALYERGFNVLCEDQGFTESHQVAMDVAKLGDVSKMAKTLQYNNIILNKNLLPWDDVNDSDNPSGIRMGTQELTHRGFKEDEMDQVAEFLA"
  },
  {
    "reference": "MTSPLLISLASCLVAVNQASLIGRCDLAKVLHQEDLDGFEGYSLTDWLCLAFVESDFNITKVNENTDGSFDYGIFQINSHYWCNDYQSRTENNCQVDCQELLSPNLLAIINCAKKIVSGAGGMKNWVKWRLHCAGRPLSYWMTGCHLA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that can perform lysozyme activity in extracellular region, sperm flagellum, sperm midpiece to support fusion of sperm to egg plasma membrane involved in single fertilization, cytolysis, fertilization, metabolic process, defense response to bacterium.2. The protein must exhibit the following characteristics: May be involved sperm-egg plasma membrane adhesion and fusion during fertilization; Shows weak bacteriolytic activity against Gram-positive bacteria at physiological pH; Bacteriolytic activity is pH-dependent, with a maximum at around pH 5.6 ..3. The active site of the protein must be engineered to enable effective catalysis of Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic   acid and N-acetyl-D-glucosamine residues in a peptidoglycan and   between N-acetyl-D-glucosamine residues in chitodextrins..4. Include a signal peptide as an essential component of the protein.5. The protein contains novel C-type lysozyme domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MDYKVHQDSEAQNMNGASDSSGNVSSSSLGSQSGGPQGPTGTPCEEDYSWVPPGLKPDQVYQYFSCLSPEQVPRLNSLCHIPCSALLSSDITASVNRVLLPEEKNAYCRMLGEEPGECKISCSALLSSDITASVNRVLLPEEKNAYCRMLGEDPGEDPYYASQIPKEERPCSTCCNIMCSKLLDDNIDDDISRCHLSCSALLQDNIADAVANCNIMCSKFLDDNIDDDIADAMEEALRVNHFEVAEFLKQHSDNKLGMGTDESDDSSEEEEEPSKKEKPRV",
    "response#2": "MSSFFRRRKDGAPPVPAPPKKSSSPSPSSSSSAPSSSSSASSASSSSSASSSSASSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS",
    "response#3": "MPALSPTMTDGTIVKWLKKEGESVNPGDVIAEIETDKATVEMESFDEGILAKILVAEDTKNVPIGKVIGLIAEEGEDVSKITASVAAAPAAAAPAPAPAAAPAAAPAASNGRVYASPLVRKLAAENGVDLSAVSGSGPRGRIVKSDVEAALSGKAPAAAPSPAPAPAPVAAPTPAPAAAPAPAPAAAAAPSFDDEQVPLSGVRRTIAKRLTQSKQEIPHYYLTMDCRVDKLMELRSKLNELNEDGVKISVNDMIVKALAMTLKQVPEANASWSDSGIIRHNSADIGVAVALDDGLITPVIRDADKKGITGISTEMKDLAAKARNKKLKPSEYQGGSFTISNLGMFGIDEFTAIINPPQSGILAVGSSEKAVPVVDGKPEIATKMKVRLTLSADHRAIDGAVGAKFLQTLKAYLENPMLLLT"
  },
  {
    "reference": "MLPAPLLGAAETPPPPAAAPAAAAPPGSPISATPPYSTQVGVIRCPVCSQECAERHIIDNFFVKDTTEVPSSTVEKSNQVCTSCEDNAEANGFCVECVEWLCKTCIRAHQRVKFTKDHTVRQKEEVSPEAVGVTSQRPVFCPYHKKEQLKLYCETCDKLTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITKLMEKTKYIKFTGNQIQNRIIEVNQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVAGLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNNTIQFHCDPSFWAQNIFNLGSLVIEDKESQPHLPKQNPVVEQNSQPPGGLSSNQLSKFPTQISLAQLRLQHMQQQVMAQRQQVQRRPAPVGLPNPRMQGPIQQPSISHQQPPPRLINFQNHNPKPNGPVLPPHPPQLRYPPNQNIPRQAIKPNPLQMAFLAQQAIKQWQISSGPASTVPSTASSTTSTPSSPTITSAAGYDAKSFGPPMIDLSSPVGSSYNLPSLPDIDCSSTLVLDNLVRKDVSVEHNQPRPPSNRTVQSPNSSVPSPGLPGPVTMTSVHPPIRSPSASSVGSRGSSGSSSKPTGADSTHKVPVVMLEPIRIKQENSGPHENYDFPIVVVKQETDEEPRSQNTNFPRSILTSLLLDSNHNTTSEEAALRTDAPDSTGDRPGLHTSPSNGKSGWGGTSQKSPLHGGESRKEDDPNEDWCAVCQNGGELLCCEKCPKVFH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should contain one or more Bromo domains that are essential for its function.2. The designed protein should have lysine-acetylated histone binding, zinc ion binding, chromatin binding within cytoplasm, male germ cell nucleus, nucleoplasm, euchromatin to regulate regulation of signal transduction by p53 class mediator, epithelial cell proliferation, positive regulation of gene expression, regulation of vitamin D receptor signaling pathway. The designed protein sequence is ",
    "response#1": "EGSNMIKTIAFGRYELDTWYHSPYPEEYARLGRLYMCEFCLKYMKSQTILRRHMAKCVWKHPPGDEIYRKGSISVFEVDGKKNKIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVMTEADNTGCHLIGYFSKEKNSFLNYNVSCILTMPQYMRQGYGKMLIDFSYLLSKVEEKVGSPERPLSDLGLISYRSYWKEVLLRYLHNFQGKEISIKEISQETAVNPVDIVSTLQALQMLKYWKGKHLVLKRQDLIDEWIAKEAKRSNSNKTMDPSCLKWTC",
    "response#2": "EGSNMIKTIAFGRYELDTWYHSPYPEEYARLGRLYMCEFCLKYMKSQTILRRHMAKCVWKHPPGDEIYRKGSISVFEVDGKKNKIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVMTEADNTGCHLIGYFSKEKNSFLNYNVSCILTMPQYMRQGYGKMLIDFSYLLSKVEEKVGSPERPLSDLGLISYRSYWKEVLLRYLHNFQGKEISIKEISQETAVNPVDIVSTLQALQMLKYWKGKHLVLKRQDLIDEWIAKEAKRSNSNKTMDPSCLKWTC",
    "response#3": "EGSNMIKTIAFGRYELDTWYHSPYPEEYARLGRLYMCEFCLKYMKSQTILRRHMAKCVWKHPPGDEIYRKGSISVFEVDGKKNKIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVMTEADNTGCHLIGYFSKEKNSFLNYNVSCILTMPQYMRQGYGKMLIDFSYLLSKVEEKVGSPERPLSDLGLISYRSYWKEVLLRYLHNFQGKEISIKEISQETAVNPVDIVSTLQALQMLKYWKGKHLVLKRQDLIDEWIAKEAKRSNSNKTMDPSCLKWTC"
  },
  {
    "reference": "MADSMHMRPLSDLLGFDGDGDMAITGLTSDSRRVQKGYLFAALPGSQVDGQGFIPDAVARGAVAVLGPKGLKLPGDVARIEAAVPRQAFAHMASWFYGPSPEVMACVTGTNGKTSVASFTRQIWEQSGLVAASIGTLGVESTVMTRPGGLTSPDPVALHEALASLQKAGVTHGVCEASSHGLVQYRLDGVRPKAVAFTNLSRDHLDYHHTLEAYFHAKARLFSDLAADDGAAVVFIDSEAGVRMADIAAGRGLRLITVGRAPYASIRLDGIKAEGSGQRLKIVYDAVTHDIILPLFGRFQAENALVAAGLAIGCGGDPKAAFKALETLKPVPGRLEKIGDTQSGAPVFVDYAHTPDGLKTVLEALRPHTANRLAVVFGCGGDRDRGKRPEMGVLAQDLADLVVVSDDNPRSEDAAAIRRDILAACPDAREIGDRAQAIAFAIQSLNAGDCLVIAGKGHEQGQIIGDRILPFSDIAVVNALLAGRGA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. The protein should be able to bind meso-2,6-diaminoheptanedioate, ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate ligand in a variety of conditions3. The designed protein should be involved in cell division, peptidoglycan biosynthetic process, cell cycle, cell wall organization by localizing to cytoplasm and exhibiting UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity.4. A protein that is capable of catalyzing reactions within the peptidoglycan biosynthesis pathway with high efficiency and specificity.5. The designed protein should contain one or more Mur ligase C-terminal, Mur ligase N-terminal catalytic domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MKLNELISVEGIAHDSRRVKPGDLFVALRGTKEDGHRFVPDALKAGASALLLEEPLPWAVPVPWVVVPDAREALASVAARFFGRQPETMIGVTGTNGKTTTARLVTSMLNASGHKAGLMGTVAYYIGNERIEAPNTTPDSVELHRCLAEMLDKGLKDVVMEVSSHALELSRVHGCDFDVAVFTNLTQDHLDYHGTMEAYFQSKLKLLTPLHQRAVINIDDPYGRRLMDLAPCPVVSYSVHDEADLRADNIKVTATGTSFVLQGPLGSFPLQLPLVGRHNVYNALAAIAAGIAEGVPLPEIISGLEALPGVPGRMERIWSHDRHRCAVVDYAHTPDALQKALEALRTVTPAGAKLAVVFGCGGNRDRQKRPEMGRIAAELADRVILTSDNPRDENPEAILDEIEAGMAGRAHLRIADRAEAIRRAVGQLGEGDILLVAGKGHEDYQIIGTEKVHFDDREQAREALRERR",
    "response#2": "MNDIINITYDSRKVKNGSLFIAISGENKDGHDYILEAIEKGASAIIANGRAPITNLVPILQVDNPRKTMSKVAANYYNHPSEKIKVIGITGTKGKTTTTFMIKEILEKAGKKVGLIGTVATYINGNKIGDSDRTTPESLELQKLFNKMVEEKVEYVVMEVSSQSLKLHRVDGCEFDLVAFTNFSEDHISEKEHPDMQDYFQSKLKLFNMCKTGFVNIDDLQGNKIPKMFPENDITGYGIDNSGTFLAKDITITNSYVDFKVKITDRNERVKVDIPGRFTVYNALCAICTGATLRAQQAQPGDLFAGLPGARAHGAQFIAQAVEGGAIALFTDAAGAELPEVRAAGLPVLVHPDPRAVLGELAARIAGLEADAIADGIEAVHGVPGRMQALAAGTGPTVVIDYAHTPDALENALAALRAHLPGSGHLWCLFGCGGDRDRGKRPLMAAAAERHADRLVVTDDNPRSESPGAIRGEILDGLSAEARGAARARDGRAAAIREAIREAADEDVILIAGKGHETYQIFKDTKTHFDDREVARAALQKRAK",
    "response#3": "MTTGATLRAQQAQPGDLFAGLPGARAHGAQFIAQAVEGGAIALFTDAAGAELPEVRAAGLPVLVHPDPRAVLGELAARIYGEPGRRMRVVGVTGTNGKTSVCHFTQQLWQMLGFRSTSIGTLGVMGSLEFELKNTTPDPIVLHQILRNLVMNDIDYVSMEASSHGLEQFRLDGVSFKAAAFTNLSRDHLDYHLDEDDYLASKCLLFDRVLSPGNIVVLNLDDPCAQLVKLVSENNGHKVITVGVHDSANLKICDQRYDSDGQTLKISYQGFSQIVRIPLIGNFQATNVLISAAIGLAYGLDAEQVAAGLSAVSGAPGRLQLAGRHPAGARVYVDYAHTPDALGNVLTALRPHTRGKLVVVFGCGGDRDRGKRPLMGQVAAELADRVFVTDDNPRSEVPALIRQEILASCPTAHEIGDRREAIRAAVASLAADDVLVVAGKGHEDYQIIGHTKHHFDDREEARRAIRARTTG"
  },
  {
    "reference": "MSPFQNFMILEEKQLLILMKTNSEICKELVSNPEKFNKLEQFVSLVEEWNKKINLTGFEGENLWKEGINESFFCFEKILETKNLNDFENKSWVDIGSGAGFPIIPFAIIYPKINFYIIESNSKRVRFLELVNEKLNLKIKIFNTRAENFSELKFDFVSARAVAHLDLLIKYFMKITKQDATGYFIKGPKIFEEKEEINNKKISIETLKIDKIKEKNVFVVKMNRI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTCT",
    "response#2": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTCA",
    "response#3": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC"
  },
  {
    "reference": "MKVSKAIVALAALCMALLAPAAGSKELTNIDGDLNNRHLRQESAELATTPEEIGVKKDSNNPLQRRDQALVSAHRVYDPVSGLACALVGDCMACPTSEKDETFCRETGYRQELDCPRPKDPKDAALLTKPEDEREIRFKACSPADTARPGVEVVKFEVRNVLS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should include a RxLR-dEER motif to ensure proper function. The designed protein sequence is ",
    "response#1": "DPERKEFLDDLFVFMQKRGTPINRIPIMAKQILDLYMLYKLVTEKGGLVEIINKKIWREITKGLNLPTSITSAAFTLRTQYMKYLYAYECEKR",
    "response#2": "DPERKEFLDDLFVFMQKRGTPINRIPIMAKQILDLYMLYKLVTEKGGLVEIINKKIWREITKGLNLPTSITSAAFTLRTQYMKYLYAYECEKS",
    "response#3": "PERKEFLDDLFVFMQKRGTPINRIPIMAKQILDLYMLYKLVTEKGGLVEIINKKIWREITKGLNLPTSITSAAFTLRTQYMKYLYAYECEKKR"
  },
  {
    "reference": "MYRMQLLSCIALSLALVTNSAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be involved in positive regulation of regulatory T cell differentiation, positive regulation of interleukin-17 production, negative regulation of protein phosphorylation by localizing to extracellular region, extracellular space and exhibiting interleukin-2 receptor binding.2. The design objective is to achieve a protein with helix-helix-strand-helix-helix-strand-helix-helix-helix-helix-helix-strand-helix-helix-strand characteristics.3. For general function, the protein need meet that In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5; Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells.; Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance; Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG); Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production The designed protein sequence is ",
    "response#1": "LEWHENLGHGSFTKIYRGCRHEVVDGEARKTEVLLKVMDAKHKNCMESFLEAASLMSQVSYRHLVLLHGVCMAGDSTMVQEFVHLGAIDMYLRKRGHLVPASWKLQVVKQLAYALNYLEDKGLPHGNVSARKVLLAREGADGSPPFIKLSDPGVSPAVLSLEMLTDRIPWVAPECLREAQTLSLEADKWGFGATVWEVFSGVTMPISALDPAKKLQFYEDRQQLPAPKWTELALLIQQCMAYEPVQRL",
    "response#2": "GALHVLLPARGPGPPQRLSFSFGDHLAEDLCVQAAKASGILPVYHSLFALATEDLSCWFPPSHIFSVEDASTQVLLYRIRFYFPNWFGLEKCHRFGLRKDLASAILDLPVLEHLFAQHRSDLVSGRLPVGLSLKEQGECLSLAVLDLARMAREQAQRPGELLKTVSYKACLPPSLRDLIQGLSFVTRRRIRRTVRRALRRVAACQADRHSLMAKYIMDLERLDPAGAAETFHVGLPGALGGHDGLGLLRVAGDGGIAWTQGEQEVLQPFCDFPEIVDISIKQAPRVGPAGEHRLVTVTRTDNQILEAEFPGLPEALSFVALVDGYFRLTTDSKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARAEQCHGPITLDFAINKLKTGGSRPGSYVLRRSPQDFDSFLLTVCVQNPLGPDYKGCLIRRSPTGTFLLVGLSRPHSSLRELLATCWDGGLAGPQPGDASAILDLPVLEHLFAQHRSDLVSGRLPVGLSLKEQGECLSLAVLDLARMAREQAQRPGELLKTVSYKACLPPSLRDLIQGLSFVTRRRIRRTVRRALRRVAACQADRHSLMAKYIMDLERRPSRLPSLLEWHENLGHGSFTKIYRGCRHEVVDGEARKTEVLLKVMDAKHKNCMESFLEAASLMSQVSYRHLVLLHGVCMAGDSTMVQEFVHLGAIDMYLRKRGHLVPASWKLQVVKQLAYALNYLEDKGLPHGNVSARKVLLAREGADGSPPFIKLSDPGVSPAVLSLEMLTDRIPWVAPECLREAQTLSLEADKWGFGATVWEVFSGVTMPISALDPAKKLQFYEDRQQLPAPKWTELALLIQQCMAYEPVQR",
    "response#3": "GALHVLLPARGPGPPQRLSFSFGDHLAEDLCVQAAKASGILPVYHSLFALATEDLSCWFPPSHIFSVEDASTQVLLYRIRFYFPNWFGLEKCHRFGLRKDLASAILDLPVLEHLFAQHRSDLVSGRLPVGLSLKEQGECLSLAVLDLARMAREQAQRPGELLKTVSYKACLPPSLRDLIQGLSFVTRRRIRRTVRRALRRVAACQADRHSLMAKYIMDLERLDPAGAAETFHVGLPGALGGHDGLGLLRVAGDGGIAWTQGEQEVLQPFCDFPEIVDISIKQAPRVGPAGEHRLVTVTRTDNQILEAEFPGLPEALSFVALVDGYFRLTTDSKSFLKCEAKNYSGRFTCWWLTAISTDLKFSVKSSRGSTDPRGVTCGTATLSEDLGEYKKYRVECQEGSACPAAEESLPIEVVLEAVD"
  },
  {
    "reference": "MNRLLTREEQQELDREFCKLTGLPSAVLMEQAAAGLAGLTGSLLDSFETTEADEIEVLIITGTGNNGGDGWAAARQLQAAGYRVAVLEAHPGRKMSGDAAVNRQAYGILGGRVINSLDELSEKYPLVTIDALFGTGFKTDKELPEEALAALKALAALKEAGSLIIACDIPSGVDANTGQAIRETVEADFTCTFGRKKIGLITHPGLLYAGEVLEFPLGMTDGFVEKVLEGRRRVTELNEETAGGPVEARQPDGHKGQFGRLLLIGGHEGMTGALILAARAGEKMGVGYTMIRAPEKSLPIIAGAVPSALLSAVPEEGEETKHELPEPDAIAVGPGAGSDPWVEKAIQHLLGQPMPIIIDADGLNALARMKNASSLLKERSRKGFPPAVLTPHPGEYLRLAPKNEKLLHQDRLEAARRLAEQTASIIVLKGMATVIAMPDGKAYINTTGNVGLAKGGSGDVLTGMIAGLAAQCDSTENAVRRAVYLHGLAADIASERCLSEIAVTPEDLIDSLGEALLC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be able to bind K(+) ligand in a variety of conditions2. The designed protein must be optimized for ADP-dependent NAD(P)H-hydrate dehydratase activity, ATP binding.3. The protein should be designed to have high stability and activity towards (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "MVASMIPGREKQTNKGDYGKVLIISGSKGMTGAGCLAARAAHRSGAGLVYLAVPGSLLPVYGASLTETIIIPVEDQDKGHIVYDGMGFLEKQMEGKNVIAAGPGLSVNEDTIELIFRIIEKSIMPLVLDADALNAIARDVSVLKKLKTTAVITPHPGEMARLTGKSTAQIQEDRVNTAREFASKWGVITVLKGFRTIVALPDETAYINLTGNPGMATAGTGDVLTGIIAGLITQGIKPGDAAVAGVYLHGLAGDMAAAKKGEYGLIASDLVDEIPAG",
    "response#2": "TPALWLPKFPWPTAASHKHARGRMVVVSGPAHSTGAARLAARGGLRIGAGLVTVLSPLDAVAANAAHLEAVMLRPFDTDADLESLAADVDSAVIGPAAGVGETTLMNVLALARTGAALILDADAITVFRQDPEELFSVLDRDDVLTPHPGEFERLFPGELEASPERITAARNAARTADAVVLLKGADTVVAAPDGRAAVNLNGSPWLATAGTGDVLAGFVAGLVAQGMDSFEAACAAVWIHAEAAQAHGPGLISEDLPGLAPAVLQDLFSNGTQTLVLTPHPGEMSRLIGLSTAAIQRDRVNVARNFAQEHGLILVLKGDRTIVAGPDGEAWVNPTGNPGMATGGTGDILTGIVAGLLVQFPKRAFEAVIAAVYLHGLAGDVAVESMGEQALVAMDLVAR",
    "response#3": "LCIGGNRGTPGAIRLSAEAAMRTGAGLVKVFAHEQSRIQVSAGCPELMVTNESLDEALAWCTTVVLGPGLGQDEWAKEIFERTMSYCEGAHKPMVLDADALNLLSHSSVSYNVSECLITPHAGEAARLLGVSVDEIESDRFNCARQLAQKYHATCVLKGAGSVVDTERHAYVCQHGNPGMATAGMGDVLTGILGALLAQGIDQDLACKYGVSLHAKAGDCVAEQFGERGMMASDLFHYR"
  },
  {
    "reference": "MNNVTRNLLLWLVIAAVLMSIFNNFSMQQPTEQVGYSDFISEVRNDRVRSVLVEGLTINAERFDGSRFETVRPMIEDPRLMDDMLEHQVIVEGKKPEQQSIWMQLLVASFPILIIIAVFMFFMRQMQGGGGGRGGPMSFGKSKARLLGEDQITTTFADVAGVDEAKEDVQELVEFLRDPSRFQKLGGRIPRGVLMAGQPGTGKTLLAKAIAGEAKVPFFSISGSDFVEMFVGVGASRVRDMFEQAKKQAPCIIFIDEIDAVGRHRGTGMGGGHDEREQTLNQLLVEMDGFEANDGVIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDIRGREQILKVHMRKVPLREDVEPSKIARGTPGFSGADLANLVNEAALFAARSGARKVGMQQFELAKDKIMMGAERKSMVMSESEKRNTAYHEAGHAIVGRIVPEHDPVYKVSIIPRGRALGVTMFLPEEDRYSHSRRFIISSICSLFGGRIAEEMTLGKDGVTTGASNDIQRATQMARKMVTQWGLSEKLGPLMYDQAEEEVFLGRTAGQHQKSLSSDTAKLIDQEVRSIIDECYGTAQKILEENIDKLHSMADALMHYETIDSDQIDDIMAGREVREPKDWGDSSSGDGGASTGSESKATDSASPIGGPASEH",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The protein must have high specificity towards ATP-dependent peptidase activity.3. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.4. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins The designed protein sequence is ",
    "response#1": "VLLVGPPGTGKTMLAKAVAGEAGVPFFGLSGSDFVEMYVGVGAARVRDMFQQALQRSPAIIFIDELDALGKVRGSGAPGGHDEREQTLNALLVEMDGFGTDHSVIVMGATNRPETLDPALMRPGRFDRHVLVA",
    "response#2": "VLLVGPPGTGKTMLAKAVAGEAGVPFFGLSGSDFVEMYVGVGAARVRDMFQQALQRSPAIIFIDELDALGKVRGSGAPGGHDEREQTLNALLVEMDGFGTDHSVIVMGATNRPETLDPALMRPGRFDRHVLVA",
    "response#3": "VLLVGPPGTGKTMLAKAVAGEAGVPFFGLSGSDFVEMYVGVGAARVRDMFQQALQRSPAIIFIDELDALGKVRGSGAPGGHDEREQTLNALLVEMDGFGTDHSVIVMGATNRPETLDPALMRPGRFDRHVLVA"
  },
  {
    "reference": "MGYKFLKLASASFRNGGVRIVSRQYSTTHDADLVVIGAGPGGYVAAIKAAQLGMKVVSVEKEPSLGGTCLNVGCIPSKALLHNTHLYHMAKHDFKHRGIETGEVKFNFKAMMDYKVNAVKALTGGIAMLFQKNKVKLVRGAGTIVAPNKVEVKGEKGVETVNTKNILIATGSEVTPFPGVTFDEKQIITSTGALSLESVPKKMLVIGAGVIGLELGSVYQRLGADVTAIEFLGSIGGIGIDMEVSKDYRILAKQGMKFKLETKVLGVKKEGSTVKVEDVSIEGAKGGNKETMDCDVVLISIGRRPYTKDLGLDKVGIALDDRGRVPVNNKFQTTVPGIYAIGDVIHGPMLAHKAEDEGIVCVEGIKGMPVHFNYDAIPSVIYTSPEVGWVRKTEEDLKKEGKAYKVRKFPFLANSRAKTNGEPDGFVKVLSDKATDVILGTHIIGPGGGELINEAVLAQEYGAAAEDVARVCHAHPTCAEALREANLAAYCGKPINF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a low Km value for (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + NAD(+) = (R)-N(6)-   lipoyl-L-lysyl-[protein] + H(+) + NADH.2. The protein must be able to dihydrolipoyl dehydrogenase activity. The designed protein sequence is ",
    "response#1": "IPSVAYTDPEIAWVGLSEKEAENNDIDYEVSLFPWSASGRAHASNCTLGMTKLIFNKNTNKIIGGSIIGTNASELISEIGLAIEMGSDAEDISLTIHPHPTLSESIR",
    "response#2": "IPSVAYTDPEIAWVGLSEKEAENNDIDYEVSLFPWSASGRAHASNCTLGMTKLIFNKNTNKIIGGSIIGTNASELISEIGLAIEMGSDAEDISLTIHPHPTLSESIL",
    "response#3": "IPSVAYTDPEIAWVGLSEKEAENNDIDYEVSLFPWSASGRAHASNCTLGMTKLIFNKNTNKIIGGSIIGTNASELISEIGLAIEMGSDAEDISLTIHPHPTLSESIIN"
  },
  {
    "reference": "MFKKTVPLLSAVAIAISFSAGTGVANAQENASYEVNSPEPKSNDFWWPNKLNLEPLRQHSPESDPYGDDFDYAEAFSQLDLEQVKKDIEELMTSDQDWWPADYGHYGPFFIRMAWHGAGTYRVQDGRGGAAGAQQRFEPLNSWPDNVSLDKARRLLWPVKQKYGRDISWADLMVLTGNVALESMGFETFGFAGGREDAWEPDIVYWGPEKEWLKGDERYSGDRELENPLAAVQMGLIYVNPEGPNGKPDPLLAAKDIRDTFGRMAMNDEETVALIAGGHTFGKAHGAHKPEECLGAEPAAAGVEEQGLGWKNKCGKGNAEDTITSGLEGAWSVNPTAWTTQYLDNLFGFEWEQTKSPAGAIQWIPVDGQASNLVPDAHVEGKRHAPIMFTTDLSLKEDPEYRKIAKRFHEDPKEFELAFAKAWFKLTHRDMGPKQSYLGDMAPQEDLLWQDPIPAVDFELINENDVEQLKVAILDSGLSVPQLVRTAWASASSFRGTDMRGGANGARIALEPQMNWEANNPAELKKVLDTLKGVQEDFNDELSGDKYVSLADVIVLGGAAAIEKAGKDAGYDVTVPFEPGRTDASQEMTDVNSFSFLEPKADAFRNYYAEGNRVSPAQHMVDKADQLTLTVPEMTVLVGGLRSLDANYNDSDHGVFTDQPGTLNNDFFVNLLSMDNEWKKSSDNEAIYEGFDRKTGEQKYTATTVDLIFGSNSELRAVAEVYAMSDADEKFVNDFVQAWTKVMQLDRFDLK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must contain a signal peptide for proper functionality.2. A protein with heme binding, metal ion binding, catalase activity is needed to enhance response to oxidative stress, hydrogen peroxide catabolic process in a specific context.3. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for 2 H2O2 = 2 H2O + O2.4. The protein must exhibit the following characteristics: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity..5. The protein must bind to heme b in order to perform its enzymatic function.6. The protein should be able to bind heme b ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "ETMIWQDPIDEKTASKLDNDDINELKKQVLDSGLSVSELVRVAWGAAASYRAADKRGGVNGARIALAPQKDWQVNNPVETQKVIKVLSNIQSNFNGGMFNSSRVSLADLIVLAANAAIEKAAKDAGYAVSVPFIPGRADATQAQTDVNSFSLLELSADGFRNYFDKQNSYKSPTEMLIDKADQLNLSVPQMTVLIGGMRVLDANYQGVKHGVFTNQPGTLSNDFFVNLLDMSTVWRKSSEQGIYQGFDRQTGEPKWSATSVDLIFGSNSELRAVAEVYAA",
    "response#2": "FARAWFKLTHRDMGPKARYLGPDVPAEDLVWQDPIPAGRSDYDVAAVKARIAASGLSASDLVATAWDSARTYRGSDHRGGANGARIRLAPQKDWEGNEPQRLARVLSVLEPIAAETGASLADVIVLGGNLGVEQAAKAAGFDIEVPFAPGRGDSTAEQTDAASFDVLEPLADGFRNWQKQDYVVSPEEMLLDRAQLMGLTAPEMTTLIGGLRVIGTNHGGTKHGVFTQRKGALTNDFFVTLTDMANNWVPVGNNLYEIRDRKTGATRYTATRVDLVFGSNSILRAYAEVYAQF",
    "response#3": "ETMIWQDPIDEKTASKLDNDDINELKKQVLDSGLSVSELVRVAWGAAASYRAADKRGGVNGARIALAPQKDWQVNNPVETQKVIKVLSNIQSNFNGGMFNSSRVSLADLIVLAANAAIEKAAKDAGYAVSVPFIPGRADATQAQTDVNSFSLLELSADGFRNYFDKQNSYKSPTEMLIDKADQLNLSVPQMTVLIGGMRVLDANYQGVKHGVFTNQPGTLSNDFFVNLLDMSTVWRKSSEQGIYQGFDRQTGEPKWSATSVDLIFGSNSELRAVAEVYAD"
  },
  {
    "reference": "MDKKFDSGGIVFMVGLLLLLALQLFWTAPGVPLLAYSDFLRLVDAHAVDNLAISPTRITGELRLERAKTMLPASEAAAFGAEPAPYYFATTRVADDALPSRLASAGVRYSGALDSNWGGALLGWLAPLVLFVMVWQFMMRRGGGARDFMGIGRSRAQVYVQRETSATFNDIAGIDEAKKELQQIVSFLCHPERYRRLGGKIPKGILIVGAPGTGKTLLAKALAGEAGVPFFSISGSSFVEMFVGVGAARVRDLFEQAQRQAPCIVFIDELDALGKVRGASPVSGNDEREQTLNQLLVEMDGFEANAGVILLAATNRPETLDPALLRPGRFDRHIAIDRPDLVGRRQILAVHTRKVPLAPDVDLDELAARTPGCVGADLANIVNEAALHAAEADRPSVAMADFDEAIDRALAGSERKSRVMNAQEKQTIAWHEAGHALVAQSRAHCDPVKKVSIIPRGVAALGYTQQVPTEDRYVLRRSELLDRLDALLGGWVSEALVFGDVSTGAQNDLERATAMAWHMVARYGMSERIGLANCADHDGMARSLAGADGPRCGEQTAQRIDDEVRRLLNEAHERVAHTLAERREALDRIARRLLEREVLEHDELVALIAGESAPDGSAEQSAENPSDTKKSRSVEPAA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Plays a role in the quality control of integral membrane proteins..2. The Zn(2+), ATP binding site should be located in a region of the protein that is accessible to the ligand.3. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.4. The designed protein should be involved in protein catabolic process, proteolysis by localizing to plasma membrane and exhibiting ATP-dependent peptidase activity.5. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "VLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAKKAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIIIAATNRSDVLDPALLRPGRFDRKVLVA",
    "response#2": "VLLIGSPGTGKTLLAKAVAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFEQAKRHSPCIIFIDEIDAVGRHRGAGLGGSHDEREQTLNQILVEMDGFDTDTNVIVVAATNRPDILDPALTRPGRFDRRVIFL",
    "response#3": "IPKGVILVGPPGTGKTMLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEDAKKNAPCIVFIDEIDAVARRRGTGMGGGHDEREQTLNQLLVEMDGFGVNEGIIVMAATNRVDILDPAILRPGRFDRKVAVGRPLHFDVDASLIARGTPGFSGADLANLVNEAALFAARENRRMVSMAEFESAEDLSRFIL"
  },
  {
    "reference": "MRVKGIRRNYQHLWRGGTLLLGMLMICSAVEKLWVTVYYGVPVWKDATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLENVTEEFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLECKDANVTKVNYTREMEKEIKNCSFNITTNIRDKAQREYALFYRLDVVPLDDDNTSYRLISCNTSVITQACPKISFEPIPIHYCAPAGFAIIKCNDKTFNGTGQCRNVSTVQCTHGIRPVVSTQLLLNGSLAEEKIVIRSENLTNNAKTIIVQLTEPVSINCTRPHNNTRKSIHIGPGRAFYATGDIIGDIRQAHCNLSKTDWNNTLGQVVEKLRGLFKNKTIVFDRSSGGDPEIVMHSFNCGGEFFYCNTTQLFNSTWNWNSTWNKTEKANNTDLITLPCRIKQIVNMWQEVGKAMYAPPIRERISCSSNITGLLLTRDGGNTTNDTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRAKRRVVQREKRAVGIGALFLGFLGAAGSTMGAASVTLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTTVPWNTSWSNKSMTDIWDNMTWMQWEREIENYTSLIYTLIEESQNQQEKNEQELLALDKWASLWNWFSITHWLWYIKIFIMIVGGLIGLRIVFTVLSIVNRVRQGYSPISFQTRLPARRGPDRPEETEEDGGERDRDRSGHLVDGFLAIIWVDLRSLSIFSYHRLRDLLLI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.2. The designed protein should contain one or more Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains that are essential for its function.3. The protein design should aim to mimic or enhance the function of natural proteins involved in positive regulation of receptor clustering, potentially with improved properties such as stability, specificity or selectivity.4. For general function, the protein need meet that The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Transmembrane protein gp41: Acts as a class I viral fusion protein The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWREATTTLFCASDAKAYDTEVHNVWATHACVPTDPSPQEIPLENVTEKFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDATKTNNSSGGTKTNNSSGGAVGEGEIKNCSFNITTSMRDKVQKQYALFYKLDVVPIDNNSTNTSYRLISCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGKGLCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSDNFTNNAKTIIVQLKESVKINCTRPNNNTRKSITIGPGKAFYATXEIIGDIRQAHCNLSRVDWNETLRQIAIKLGEQFKKNTIVFNPSSGGDPEIVMHSFNCGGEFFYCDSTRLFNSTWINGTRNETEGNGSTITLPCKIKQIINLWQEVGKAMYAPPIRGQIRCTSNITGLILTRDGGNNTNETEIFRPGGGDMRDNWRSELYRYKVVKIEPLGVAPTKAKRRVVQREKR",
    "response#2": "LWVTVYYGVPVWKEANTSLFCASDAKAYDTEAHNVWATHACVPTDPDPQEVYLKNVTENFNMWKNNMVEQMHEDVISLWEQSLKPCVKLTPLCVTLNCTDYCGTNTTRNSTHPINCNTTSNKTTSDNATMMEAQGMKNCSFNITTSIRDKVKKDYALFYSIDVIKINETKDYDSYRMISCNTSIITQACPKISFEPIPIHFCAPAGFAILKCINKTFSGKGPCNNVSTVQCTHGIRPVVSTQLLLNGSLAEKEIVIRSDNFSDNAKNIIVQLNTSVVINCTRPNNNTRKSIHITPGRAFFTTGEVIGDIRRAHCNITKANWTDTLKLVADKLREQYNKTIIFKNSSGGDPEIIMHSFNCGGEFFYCNTTKLFKGRWDGKNWTDDNLEGSNETIILPCRIKQIVNLWQEVGKAMYAPPIQGLIRCSSNITGLILTRDGGNGNNSHNETFRPGGGNMKDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRXXXXXXX",
    "response#3": "LWVTVYYGVPVWKDANATLFCASDAKSYKTEAHNIWATHACVPTDPNPQELELKNVTENFNMWKNNMVEQMHEDVISLWEQSLKPCVKLTYLCVTLNCTNATSKDTSPTATSVNSSSIGEVKNCTFNVTTELIDKTKKEHALFYRLDIVEIGEKNNNTNWTTYRLINCNTSKITQACPKXTFQPIPIHYCAPAGFALLKCNDKRFNGTGPCTNVSTVQCTHGIMPVVSTQLLLNGSLAEEEIMIRSENLTDNVKNIIVQFNKSISITCVRPGNNTRRSIHFGPGRAFYANDITGDIRKAHCNISGGNXSNTLHQVAKKLRNLYNTTKIIFQPSSGGDPEIXTHSFNCDGEFFYCDTSKLFNGTWNNGTWENSTWNNTDITIPCRIKXIINMWQGVGKAIYAPPIAGQINCTSNXTGLILTRDGGXSNDSHSNKNETFRPGGGNMKDNWRSELYKYKVVRIEPLGVAPTKAKRRVVEREKRXH"
  },
  {
    "reference": "MTDVLVASRNAKKLAELHRVLRAAGVDGLRLLSLDDVPAFDEAPETGATFEENALAKARDAFAATGLASVADDSGVAVDALNGMPGVLSARWSGRHGDDAANTALLLGQLRDVPAERRGAAFVSACALVHAGGETVVRGEWRGTIADAPRGAGGFGYDPVFVPEGSDRSAAELSPEEKDAASHRGRALTLLVPALRALL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a binding site that is specific to the target Mg(2+), substrate ligand.2. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.3. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.4. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in dITP + H2O = dIMP + diphosphate + H(+) are properly positioned for optimal catalysis.5. The protein design must be able to perform XTP diphosphatase activity, metal ion binding, ribonucleoside triphosphate phosphatase activity, ITP diphosphatase activity, dITP diphosphatase activity in order to contribute to purine nucleoside triphosphate catabolic process, nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "MIHDKNDIITEALRYIKEKKMVILADDEDRENEGDFIALAEKATPDVINFMSKEGRGLICTPITEERAEELQLDLMVGNNSDPHGTAFTVSVDHVSTTTGISAFERALTIKKLVDKKAKPEDFRRPGHIFPLVAKKGGVLKRAGHTEAAVDLARMCGAYPIGVLCEIIKEDGTMARVPELVKFAKKHNLKIITIKDLINYRLKREKLIKREIAIETEMPTKYGKYYIKTKKERMGHLLSNIKND",
    "response#2": "MKIVFATKNAGKLKEIRDILGDKYDLKSLLDLEDIEEIPETGTTFEENAYKKASETAKAVGNVVLADDSGLEVDALDRAPGVYSARYAGGEDVAYADNNKKLLKELEDVPEGERRARFVCAIAVAFPDGSVKTAEGTLEGRIAFERRGENGFGYDPLFVPEGYEKTFAELGEEIKNAISHRAKAVAKLVEELK",
    "response#3": "MEIILATGNKGKVSEFREILRDLPLEIKAVSNFPNIPEVAEDGATFEENSRKKALELAKATGLLTLADDSGLEVDALGGAPGIRSARFSGENATDQENIDKLLRELKDIPWEKRTARFRCVIALAYPGGEEIVVRGECEGRIGLAPKGENGFGYDPVFFVPELGKTMAEMPMEEKNRISHRGRAVRKAREILEKWRKM"
  },
  {
    "reference": "MSAKQIAFASGSAHKLKEMRLILEPLGFQLLSPKDLSISFSPEETESTFLGNSLIKSRELHRLTGLPSFADDSGICVDALEGRPGVYSARYGGEGLSDKDRALLLLSELGNNPNRKANYTCVISFCDGKTETSFEGQVFGEISADYDEVGKYGFGYDPVFFYPPFGKRFSEVEEERKNKVSHRAVAVDQFVRWLETYGTSL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must be optimized for ITP diphosphatase activity. The designed protein sequence is ",
    "response#1": "ELRPIKITRNWLDHAAGSVLVEXGKTRVLCAASASXGVPRWRTGSGLGWVTAEYAMLPSSTHTRSDRESVKGRIGGRTHEISRLIGRSLRAVIDYKALXENTIVLDCDVLQADGGXRTAAITGAYVALADII",
    "response#2": "ELRPIKITRNWLDHAAGSVLVEXGKTRVLCAASASXGVPRWRTGSGLGWVTAEYAMLPSSTHTRSDRESVKGRIGGRTHEISRLIGRSLRAVIDYKALXENTIVLDCDVLQADGGXRTAAITGAYVALADLL",
    "response#3": "EELRPIKITRNWLDHAAGSVLVEXGKTRVLCAASASXGVPRWRTGSGLGWVTAEYAMLPSSTHTRSDRESVKGRIGGRTHEISRLIGRSLRAVIDYKALXENTIVLDCDVLQADGGXRTAAITGAYVALADLA"
  },
  {
    "reference": "MATHVETEFATIEAAIEDIRAGKFVVVVDAADRENEGDLTIAAQFATPEAINFMATHARGLICLCLTPERCDELALRPMAENNEAPLQTAFTVSIEARDGVTTGISAADRSRTIQTAIDPNKVAGDLVQPGHIFPLRARPGGVLERTGQTEAAVDLARLAGLIPAGVVCEVMNEDGTMARVPDLLEFCDRHDVRLVTVADLIEYRRRTEKLVERTISVRLPTTYGDFTAVAFREKLTGKHHVALVKGAVQGEEDVLVRVHSECLTGDVFHSLRCDCGEQLDQALRRIGAEDRGVLLYMAQEGRGIGLLSKLRAYELQEGGLDTVEANLELGFPADAREWGIGNQILADLGLTTIRILTNNPKKITGLEGFGLRVTEQVPIETEPNDENRRYLATKRDKLGHKLHHQDLRFDS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The presence of GTP cyclohydrolase II domains is critical for the interaction of the designed protein with its intended targets.2. The designed protein should be able to compete with endogenous proteins or inhibitors for GTP, Mg(2+), D-ribulose 5-phosphate binding.3. The protein's Nucleophile; for GTP cyclohydrolase activity, Proton acceptor; for GTP cyclohydrolase activity must be carefully engineered to ensure that the amino acids involved in GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate are properly positioned for optimal catalysis.4. The protein should be optimized for activity in Cofactor biosynthesis, 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. The designed protein should be optimized for GTP cyclohydrolase II activity, magnesium ion binding, GTP binding, manganese ion binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity activity in order to improve riboflavin biosynthetic process. The designed protein sequence is ",
    "response#1": "SEATMPIGDALWQILGYRDVQGNEHMALVLGDVQCEPSVLVRVHSECLTGDVFASKRCDCGAQLDLALEMIAAEGRGVVVYLRGHEGRGIGLVEKIRAYRLQDGGLDTVDANLQLGHPADARDYRVGVQILSDIGVGAMRLLTNNPAKRQAIEAHGLVIDEYVSIQPPEVAKVTEAKLPTKYGEFVAVAYKSIVDPAEHTALVKGDVSGDEPVLVRVHSECLTGDVFESLRCDCGEQIKLAMQRISGEGRGVFLYMRQEGRGIGFHNKLRAYALQDQGLDTVEANLALGFAPDLRDYGIGAQILADLGLHKIRLLTNDPKKVVGLESYGLKIVEIVREVVAQLPSEFGDFRIAAYTNLLDGKEHVALIKGEISSDEPILVRVHSECLTGDVFGSLRCDCGPQLHAALEQIEQAGSGVLLYLRQEGRGIGLINKLRAYNLQEQGFDTVEANEKLGFAADLREYGIGAQMLRDLGVRGMRLMTNNPRKIRGLEGYGLKVIERVE",
    "response#2": "MNRVAEATLPTKYGGEFKIIVYENEVDDMKHIALVKGDIQPEDEVLVRVHSECVTGDLFGSLRCDCGDQLHKAMEMVGQEGKGVIVYMHQEGRGIGLANKIRAYDLQEQGRDTVEANIELGFKEDLRDYGIGAQILVDLGVRKMRMITNNPKKIVGVEGYGLTVVERVPNPPDRMPAEANFPTRFGAFRLYLFKDKLSGEEHIALIKGDIKGKKNVLVRVHSQCFTGDVLGSLRCDCRDQMEKALKIINDEGQGVFLYMRQEGRGIGLSNKVHAYHLQDLGYDTVEANCRLGFEPDLRDYGIGAQILSELGLSSIRLLTNNPKKIVGLEGYGLKVTERVRVTEANMPTKYGDFKIVGYENKLNGEHHIALVKGNVSDGEPALVRVHSECLTGDAFGSLRCDCGEQYSAAMKKINDEGRGVLVYMRQEGRGIGLINKLKAYALQDKGMDTVEANLALGFPEDMRDYGIGAQILLDLGVQKIKLMTNNPKKLCGLSGYGIEIVDRVEANFPTRFGAFRLYLFKDKLSGEEHIALIKGDIKGKKNVLVRVHSQCFTGDVLGSLRCDCRDQMEKALKIINDEGQGVFLYMRQEGRGIGLSNKVHAYHLQDLGYDTVEANCRLGFEPDLRDYGIGAQILSELGLSSIRLLTNNPKKIVGLEGYGLKVTERVPNPPDRMPRVTVAKMPTKYGEFMAYGYRNLLNGEHHVALVKGEIRDGENLLCRVHSECLTGDAFGSLRCDCGQQFAAAMTQIEKEGRGILLYMRQEGRGIGLLNKLRAYELQDGGMDTLDANLALGFPGDMREYYIGAQILKDLGVKTMRLLTNNPDKVYQLSDFGMEITKRV",
    "response#3": "TSAMPTEFGDFRATAYRNTLDGTEHLALVMGDLAAASRTTEGALVRVHSECLTGDILGSLRCDCGGQLEQALAAIAAEGAGAVVYLRGHEGRGIGLGHKIRAYALQEQGLDTVDANTAQGLPVDSRSYGVGAQILGDLGATRIRLITNNPAKYGGLDGYGLTITGRIMPPIEVADGSTTRIPLQQHGDFNMTVFENDLDPAEHFALVKTPLFANQVPLVRIHSECITGDVFGSCKCDCGKQLESSLALIAAEGGVLVYLRQEGRGIGLANKLKAYALQEAGLDTVEANLQLGLPADDRDYAIAYQILKHLGIDTVRLITNNPSKIKALEEYGICVSERIPPYPAGEGRFRVTVAKMPTKYGEFMAYGYRNLLNGEHHVALVKGEIRDGENLLCRVHSECLTGDAFGSLRCDCGQQFAAAMTQIEKEGRGILLYMRQEGRGIGLLNKLRAYELQDGGMDTLDANLALGFPGDMREYYIGAQILKDLGVKTMRLLTNNPDKVYQLSDFGMEITKRVPPLTTADGTRVAKARMPLVHGAFTVAAYESVIDGSTHVALVCGEIGDGERVMVRVHSKCLTGDVFHSLRCDCGPQLDGAIEQIAKEGRGVLLYLNQEGRGIGLANKIRAYELQDQGMDTVQANEHLGFKADQRDYGIGAQILRDLGVRTMRLLTNNPRKFVGLEGYGLAVAETLPRPRE"
  },
  {
    "reference": "MSSAEADSVPPEVRRQWQELAETVREHQFRYYIKDAPIISDAEFDALFNELLALEERHPELRVADSPTQLVGGAGFATDFAEAAHLERMLSLDDVFDTDELIAWSNRVENEIGKDPHYLCELKIDGVALSLVYRDGRLERAATRGDGRVGEDVTLNARTIDDVPERLSPSDDFPVPALLEVRGEVFFLLADFEALNASLVEDGKAPFANPRNSAAGSLRQKNPAVTARRKLRMICHGIGRTEGFSPKTQHEAYTALSVWGLPVAEQTARVRGLAAVQERIGYWGEHRYELQHEIDGVVVKVDDVALQRRLGATSRAPRWAVAYKYPPQEAQTKLLDIRVNVGRTGRVTPFAFMTPVKVAGSTVGLATLHNAAEVKRKGVLIGDTVMIRKAGDVIPEVLGPVVDLRDGTEREFVMPTTCPECGTPLAPAKEGDADIRCPNARSCPAQLRERVFHVAGRGALDIEGLGYEAATALLKAGVIADEGDLFALTEDDLLRTELFRTKAGTLSANGTRLLQNLQKAKKVALWRVLVALSIRHVGPTAARALATEFGDLDSIMSASTERLAAVEGVGPTIAAALTEWFTVDWHRAIVDKWRGAGVRMADERDASVPRTLEGLTVVVTGSLAGFSRDDAKEAILARGGKAAGSVSKKTDYVVAGDSPGSKYDKAVELGVPILDEDGFRKLLAEGPPETPAD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for NAD(+) binding.2. For general function, the protein need meet that DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double- stranded DNA using NAD as a coenzyme and as the energy source for the reaction; It is essential for DNA replication and repair of damaged DNA.3. A protein with metal ion binding is needed to enhance DNA repair, DNA replication in a specific context.4. The protein should be designed to catalyze the following reaction NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-   (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-   nicotinamide D-nucleotide.. The designed protein sequence is ",
    "response#1": "MDKNDYLKAVDSLNLWAKAYYTDDSPMASDDEYDKLYNAVVQFEKENPDLKLSYSPTNRIGGEILDEFKKISHIEKMWSMEDIFSDSELVAWISRGDKTDCEFFAEPKFDGASLNLLYENGNLISAATRGDGSVGEDVTNNAKVISSVPLQIDYKDKIEIRGEVVITKDDFELINIERSKNSEPPLANPRNAAAGSLRQLDNGIVKQRKLKFYPWGVGENSLKFNKHSQIMDFVRSLGFLHDNFCKVIRGIDELKIAYKELLSVRELKPILMDGMVIRVNDLSKANQLGYTVKFPKFMVAYKFPATEKTARLIDILLQVGRTGVVTPVGLLDGVLIDGAFIKNVTLHNFDEIDRLNLMKNDFVTVIRSGDVIPKITGVFKDRRDGSQSEIIRPKICPKCGNELLDEGVFIKCQNLTCKARVVSSIIYFASKKKLLFGLGIRHVGAKAAKSLAVHFETMDNLKVADKETLTSINDIGEKMADSIVTYFANEEVHDKLFTSVTHASQMMSLDNTYSSEEVLRWHERCVKSLGHGNFEMVAEAKIDGVSCSLTYEGGVLTVAATRGDGKTGEDVTANIKTIKTIPHNISFKNKIEIRGEIYIEKADLKRLNEIQENEGENIFANTRNAAAGSIRQKNAEISASRPLKFFAHSFGYGDVDVKGFAEFMDLCKSWGFSVTPNRIRTADINEITSFYKRFEELRAGLPYDVDGIVVKINSFAEQKILGVTAKSPRWAIAFKYKPEPLPKDNYKFCPGCGALLVRALDDANWRCTNDDCP",
    "response#2": "MDKNDYLKAVDSLNLWAKAYYTDDSPMASDDEYDKLYNAVVQFEKENPDLKLSYSPTNRIGGEILDEFKKISHIEKMWSMEDIFSDSELVAWISRGDKTDCEFFAEPKFDGASLNLLYENGNLISAATRGDGSVGEDVTNNAKVISSVPLQIDYKDKIEIRGEVVITKDDFELINIERSKNSEPPLANPRNAAAGSLRQLDNGIVKQRKLKFYPWGVGENSLKFNKHSQIMDFVRSLGFLHDNFCKVIRGIDELKIAYKELLSVRELKPILMDGMVIRVNDLSKANQLGYTVKFPKFMVAYKFPATEKTARLIDILLQVGRTGVVTPVGLLDGVLIDGAFIKNVTLHNFDEIDRLNLMKNDFVTVIRSGDVIPKITGVFKDRRDGSQSEIIRPKICPKCGNELLDEGVFIKCQNLTCKARVVSSIIYFASKKARLIDILLQVGRTGVVTPVGLLDGVLIDGAFIKNVTLHNFDEIDRLNLMKNDFVTVIRSGDVIPKITGVFKDRRDGLARLIDILLQVGRTGVVTPVGLLDGVLIDGAFIKNVTLHNFDEIDRLNLMKNDFVTVIRSGDVIPKITGVFKDRRDGARPEQPFAGQSWCVTGSFENFQPRDKAMDLIVYYGGRKVSAVSSKTTHLLAGPGAGSKLEKANELGVSVYDEKQFLDLK",
    "response#3": "MKKEEYLEKVALANLWMRAYYEKDEPLASDEEYDALIRELRVFEEQNKDEISKDSPTQKIAPTIQSEFKKIAHLKRMWSMEDVFDESELRAWAKRAKCEKNFFIEPKFDGASLNLLYENGKLVSGATRGDGEVGEDITLNVFEIENIPKNIAYKERIEIRGEVVILKDDFEKINEKRALLNQSLFANPRNAASGSLRQLDTSITKERNLKFYPWGVGENTLNFTKHSEVMQFIRELGFLKDDFVRLCANLDEVLKAYDELLALREKKPMMMDGMVVRVDDLALCEELGYTVKFPKFMAAFKFPALEKTTRLIGVNLQVGRSGVITPVAVLEPVNLDGVVVKSATLHNFDEIARLDVKINDFVSVIRSGDVIPKITKVFKERREGLEMEISRPKLCPTCQSELLDEGTLIKCQNIDCEDRLVNSIIHFVSKKAMDVDGLGEKIIDQLVELNLVKTPADLYFLKKGKLTGLDRMGPKSAQNLIASIEKSKKTTLAKFIFSLGIKNIGEKTAYMLAQQFKSFANWFDKMSMLKDDTQTEDEIRNLDGMGSCICESLLDFFSDSDNCNMIEKFQKVKHLSPMLSLRNIFSEEELVEYIEKTKRFLNLKSALEFLCEPKIDGVSFSARYVNGKLVSCATRGDGKIGENIIDNMKVINGFPIEIMDVPDLLEVRGEVFLDHDTFQTLEGFSNPRNAAAGSLRQLNPEITNERNLQYFAYSASKIDGIEHQEDVLHFLSGRGFMTNPLRLASSKVPEIMSFYDSVYRRRSQIKYDIDGLVYKVNDLKLHARLGTLSDAPRWAIAHKFP"
  },
  {
    "reference": "MLDVKMIRQNPDFVKEQLGHRGVAASDIDDLLAADEKRRELIAKSEQLKSTRNQVSGEISQKKRNKEDASAEIAEMQKVSADVKTLDEERRTVDAQVQDMAAHLPNMPHPDVPVSLKEEDAVELRRIGTPRHFDFTPKAHWDIGEDLGILDFERGAKVSGSRFLYYVGDGAKLERAVYNFFLDQHEAEGYTEVLPPYMVTDESMYGTGQFPKFKEDAFRITTQDLTLIPTAEVPLVNYYRDEVIPAEKLPVYFTALSPAFREEAGSAGRDTKGLIRLHQFNKVEMVKFTKPEDSWDELEKLTNNAESLLKKLGLPYHVITLTTGDMSFTAAMTHDLEVWFPEQDKYREISSCSNCTDFQARRAHIQYRDDDGKLQFVHTLNGSGLAVGRTVAAILENYQNEDGTVTIPDVLVPYMHGKTKIEKQG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be optimized for serine-tRNA ligase activity activity in order to improve selenocysteinyl-tRNA(Sec) biosynthetic process, selenocysteine biosynthetic process. The designed protein sequence is ",
    "response#1": "DHVELGEALGIIDFDAGAKTTGSKFYFLRGEAVLLELGLVRFALDLLAGRGYELAMTPDLARDRMLLGTGFVPRGPETQIYSVENTDLSLIATAEITLAGQLVDEIVAEESLPRRFAGLSHCFRTEAGAHGRASRGLYRVHQFTKVEMFAFTTPEQSGEMHEEMLSIEEEIFRTLEIPYRVVDICTGDLGAAAYRKYDVEAWMPGRGDYGEVTSTSNTTDYQARRLGIRYRPEGGRPRLLHTLNGTAIAVSRTMIALLENHQREDGSVALY",
    "response#2": "DNVELRRWGSPRQEEGLEEHWQIAERLHLFDTERSVRIAQSRFVTLMGQGARLERALINFMLDLHTSKGYREVMPPVLVNSASLTGSGQLPKFADDCFRCSEDDLWLTPTAEVPVTSLHRDEIIPADQLPLRYAAYSPCFRREAGSYGRDTRGLIRLHQFNKVELYWFAHPDHSAEAHAQITADAEAVLQALELPYRVLDLCTADIGFSAQRTYDLEVWLPGAEAYREISSCSVCGDFQARRSAIRTKEGKSTKLVHTLNGSGLAVGRTMAAVLETGQQSDGSVLLPKALVPV",
    "response#3": "NVDHHRFGAKRDYAFTPKAHYDLGEALGMMDFEAAAKISGARFVVLKKGLARLERAIGQFFLDVHTGEHGYTEVNPPLLVRDDAMFGTAQLPKFREDQFQTVTEEVRRRTIVEFVSSAMKLGASATDAGGSEFARQLANEELVDFSYAKMPERFWLIPTAEVSLTNLVRESILDEKELPMRLTALTPCFRAEAGAAGRDTRGMIRQHQFTKVELVSITTPEQSKDEHERMLSCAEEVLRRLGLHYRVMTLCTGDMGFASQKTYDIEVWMPGQGEGGAYREISSCSVCGDFQARRMDARSRGPDGKPRFVHTLNGSGTAVGRALIAVIENYQQEDGSIAVPDALQPYR"
  },
  {
    "reference": "MAIKVLTPEEMRRADRKAIEEHGIPSLTLMERAGSGLAFFLEELYGERLKSAKVAIFCGKGNNGGDGLVSAIHLHKKAKELNVFLLCDETEFSGDSQHQLKRALDKGIQVKSFKEFLKENQDFDIYIDAIFGTGFRGKLENQYLEVIEKINSQNGLKIACDIPSGVNGETGDVENTAFKADYTVTFAFPKTGLLLYPGKYYAGKIKIIDIGIPENIVDSNKFLLEREDIKNFLPEYRGDEHKGVCGKVLVVSGSKEFTGAAFFAAEASIYSGAGLVYLAVPEEIRPVMQTKCNEVIIRSYTGIEEFKQICKGDYDVIAFGPGLGRNQNTILLLQEILKLKAPKVIDADGIWALAQLNAELTPADIVTPHPGEASFLLKGTSPTEINRNRIKYVQELSLKLKATVILKGAPTVVALKNSVFFNSTGNPGMAVGGMGDVLTGILAGLYPRIKDSLKTALSGVFLHGLSADLLLEQDTFETITPTKVLNNLNLAFRKVRS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have a high specificity for K(+), AMP, (6S)-NADPHX binding, with minimal off-target effects.2. The designed protein must be able to perform ADP-dependent NAD(P)H-hydrate dehydratase activity under specific conditions.3. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. The designed protein sequence is ",
    "response#1": "TPHVVRTYLPKRQPSSHKGTFGHAGIIAGSVGKTGAAAMAAQAALRVGAGLVTVAIPTSVNDVLEAKLLEAMTIPMPETKARTLARTAFDRLVGFMEARTAIAIGPGLSTHPETVELVRALTKQLDRPAVLDADALNALTGRTALLAACKTPPIITPHPGEMARLEADATPQTVNSDRIGTATRFARERGLFVVLKGARTVVARPDGVVAICPTGNPGMATAGTGDVLTGMIVGLLAQGVSSWEAACAATYLHGVAGDLASARKGQAGLISSDVIEEIPCALKLMHA",
    "response#2": "GIIAGSVGKTGAAAMAAQAALRVGAGLVTVAIPTSVNDVLEAKLLEAMTIPMPETKARTLARTAFDRLVGFMEARTAIAIGPGLSTHPETVELVRALTKQLDRPAVLDADALNALTGRTALLAACKTPPIITPHPGEMARLEADATPQTVNSDRIGTATRFARERGLFVVLKGARTVVARPDGVVAICPTGNPGMATAGTGDVLTGMIVGLLAQGVSSWEAACAATYLHGVAGDLASARKGQAGLISSDVIEEII",
    "response#3": "IPPEEMSAIDANCKYFGLLPLQLMENAGASLAGATKTKARGKKIAIVAGKGNNGGDAFVAARHLAGFEVTVFLLGRSKDIATEEARRNWDILEKLGFDIREIGDVRDMNIGEYDLIIDAIFGTGVREPILGLEGAAIDAINFASLPVISVDVPSGLGTSKMVRPDLTVTFHKPKPGITGEVMVEDIGIESNKGTFGRVMLFCGSPPYPGSAYLAGSAVLRVGAGLVTLAVTPDLLPIYASSLHEATYVLLPAGEPGEQRGQEQARTLLEHLSGYRALLIGPGLGRAPATRSAILALLDALRSLPEDERPALVIDADGLNNLADVENWWQRLPPGTVITPHPGEMSRLLGETRVSGGGIDRLQVARHCAQAWNVILVLKGACTLISEPAGRLRINWESNPALASAGTGDVLAGMIAGYLAQRLTPFDAASLAVYLHVAASKLVSQHCGDAGLLASDLLPQII"
  },
  {
    "reference": "MEIKNLTTEQRNPASIHIDTVSTVEMVKIMNEEDQKVALAVGNQDEQIARAIDEAANRYKKGGRLIYLGAGTSGRLGVLDAAELVPTYGIKPERAIGLIAGGPGAMYKAVEGAEDDTNLGAEDLKDLNLNSQDIVLGLAASGRTPYVIGGLEYANQIGAFTISIACVKDSEIGKHAEVAIEAVVGPEIVTGSTRMKSGTAQKMILNMISTGVMIRQGKVFENVMIDVMPTNSKLVDRASRIISAVTDATQEEALQTLKKAENNVPLAITMIKTESNKDEAQKLLEQYNGNVSEVIKNN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design must be focused on enhancing the efficiency of carbohydrate metabolic process. The designed protein sequence is ",
    "response#1": "LWDMYFINLVERQNIWYRRVIQGKSKPWTDDPVMSTYHFTNVDRRLDRVTLHYIDKVLCNLEDSYESKKFLLLNTFIYRLFVRPETTDAMGYIFPETFEEDWERAKAALRARRESGEPVFTDAYFVNDLKSANPDRANSSNKTENAIHLIQFIIDHLDELAEFTFNPKNSMEEVIEKFTMIPAVGNFNAYEVALDLGIVKEMTGIDFVDWTPDHYANVGPGCKKGIEYVFEDLGNMSHLDIVFFITSVYKGELERLGLEYKYQEGCKELDLRALEGWCCEMSKYFN",
    "response#2": "LWDMYFINLVERQNIWYRRVIQGKSKPWTDDPVMSTYHFTNVDRRLDRVTLHYIDKVLCNLEDSYESKKFLLLNTFIYRLFVRPETTDAMGYIFPETFEEDWERAKAALRARRESGEPVFTDAYFVNDLKSANPDRANSSNKTENAIHLIQFIIDHLDELAEFTFNPKNSMEEVIEKFTMIPAVGNFNAYEVALDLGIVKEMTGIDFVDWTPDHYANVGPGCKKGIEYVFEDLGNMSHLDIVFFITSVYKGELERLGLEYKYQEGCKELDLRALEGWCCEMSKYFN",
    "response#3": "LWDMYFINLVERQNIWYRRVIQGKSKPWTDDPVMSTYHFTNVDRRLDRVTLHYIDKVLCNLEDSYESKKFLLLNTFIYRLFVRPETTDAMGYIFPETFEEDWERAKAALRARRESGEPVFTDAYFVNDLKSANPDRANSSNKTENAIHLIQFIIDHLDELAEFTFNPKNSMEEVIEKFTMIPAVGNFNAYEVALDLGIVKEMTGIDFVDWTPDHYANVGPGCKKGIEYVFEDLGNMSHLDIVFFITSVYKGELERLGLEYKYQEGCKELDLRALEGWCCEMSKYFR"
  },
  {
    "reference": "MEPLQTYVQRLRSGEPVQFPADANSLAFAQSLDAQDSLSHLRSEFILPTKSSLKKKALNGSIPGEQEQSAEAAAADEGIYFVGNSLGAQPKAVRRHINAQLETWASIGVNGHFQTMQESPLAMWQDMAQDCADKSQDLVGASAHEIVIMNTLTANLHFLMAAFYKPNAQRHKVILEWRPFPSDHYAIESQVLWHGLDPEKSMVHIDPDEKSQISTEKILATIDEHADDTALLLLPGIQYYSGQLFDMKTITAYAKARGIVVGWDLAHAAGNVELQLHDWDVDFAAWCTYKYINAGPGAIAGAFIHERHGKVEWEMSASGARKPVFHPRLSGWYGGDKSVRFNMDNEYVPTPGASGYQVSNPSAMELACLSGALSVFNKTCMKDLRSKALVLTAYAEHLLDLMVAESKDEVPPFTVLTPRDPLQRGTQLSVLLREGLLEPTIVALEENGVMCDKRKPNVIRVAPVPLYSRFEDVYKFMQVLRKPVGLAASQ",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be able to perform kynureninase activity under specific conditions. The designed protein sequence is ",
    "response#1": "AAPLAKIVGAKPSEVTVMNTLTVNLHLLMVSFYRPEGKRFKIICEEKAFPSDQYMIKSQVRFHGYDPAEAIVELKKRDGEHHFRQEDILQTIAEVGEECALILIGGVNYYSGQVFDMKTITQAGHDIGAFVGWDLAHAAGNIALELHDWDVDFAAWCSYKYN",
    "response#2": "AAPLAKIVGAKPSEVTVMNTLTVNLHLLMVSFYRPEGKRFKIICEEKAFPSDQYMIKSQVRFHGYDPAEAIVELKKRDGEHHFRQEDILQTIAEVGEECALILIGGVNYYSGQVFDMKTITQAGHDIGAFVGWDLAHAAGNIALELHDWDVDFAAWCSYKYA",
    "response#3": "AAPLAKIVGAKPSEVTVMNTLTVNLHLLMVSFYRPEGKRFKIICEEKAFPSDQYMIKSQVRFHGYDPAEAIVELKKRDGEHHFRQEDILQTIAEVGEECALILIGGVNYYSGQVFDMKTITQAGHDIGAFVGWDLAHAAGNIALELHDWDVDFAAWCSYKYI"
  },
  {
    "reference": "MTKEPVVAILGATGAVGQEFIRLIEERKFPYSQLKLLASHRSAGKKLMVNGKEYTVEEAKPESFDGVDIGLFAGGSVSKELGPEAAKRGCVVIDNSSTFRMDPNVPLVVPEVNPEDIALHKGIIANPNCSTIIMLMALKPIHDLSPIKRIIVSTYQAVSGAGKEGLDELREETEAFLAGRDMEPKILPSKSLPKHYPIAFNLIPQIDKFVEDDYTKEEMKMVNETHKMLHDDSIAITATTVRVPVFRSHAESIYVETADELTVDAVRKAIDAFPGAQVQDDPAAMEYPMPLFTSEKYDCYVGRIRKDLYNPKAVNLWVSGDQIRKGAALNALQIAEYMLDHDMIH",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein have Semialdehyde dehydrogenase NAD-binding domains with specific sequence motifs that are crucial for its activity.2. A protein with NAD binding is needed to enhance 'de novo' L-methionine biosynthetic process in a specific context.3. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes.4. A protein should be able to manipulate the activity of enzymes in the Amino-acid biosynthesis, L-threonine biosynthesis pathway, either by activating or inhibiting their function.5. The protein should have a high affinity for substrate, NADP(+), phosphate. The designed protein sequence is ",
    "response#1": "LGGLFRNGLVEWGTSMTYQAASGGGARHMRELLNQFGTLNSAVAANLADPASAILEIDRAVLAQQKDPSMDASQFGVPLAGSVIPWIDADLGNGQSKEEWKGGAETNKILGRHTAEAARIPFDGLCVRIGAMRSHSQALTLKLTEDLSIGEIERIIDADNEWAKVVPNTKEATMAELTPVAVTGTLDIPVGRIRKLEMGPQYISAFTVGDQLLS",
    "response#2": "LGGLFRNGLVEWGTSMTYQAASGGGARHMRELLNQFGTLNSAVAANLADPASAILEIDRAVLAQQKDPSMDASQFGVPLAGSVIPWIDADLGNGQSKEEWKGGAETNKILGRHTAEAARIPFDGLCVRIGAMRSHSQALTLKLTEDLSIGEIERIIDADNEWAKVVPNTKEATMAELTPVAVTGTLDIPVGRIRKLEMGPQYISAFTVGDQLLVAR",
    "response#3": "LGGLFRNGLVEWGTSMTYQAASGGGARHMRELLNQFGTLNSAVAANLADPASAILEIDRAVLAQQKDPSMDASQFGVPLAGSVIPWIDADLGNGQSKEEWKGGAETNKILGRHTAEAARIPFDGLCVRIGAMRSHSQALTLKLTEDLSIGEIERIIDADNEWAKVVPNTKEATMAELTPVAVTGTLDIPVGRIRKLEMGPQYISAFTVGDQLLL"
  },
  {
    "reference": "MSRLIVVCIVISMICGALSLSSTKMADEKKEKDEDEKPKISGGTVIAAGRLTLDLLKTLLGTLGSISRKIAIGVDNETGGLITGNNVYFRSGTSDDILPHRVETGEALLYTARKTKGPVATGAVGVFTYYLSDGNTLAVLFSVPFDYNFYSNWWNVKIYSGKRNADYDMYHELYYDANPFEGDDTWEYRYLGYGMRMEGYMNSPGEAILKITVMPD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Pore-forming protein that forms cations-selective hydrophilic pores of around 1 nm and causes cytolysis; Pore formation is a multi- step process that involves specific recognition of membrane sphingomyelin (but neither cholesterol nor phosphatidylcholine) using aromatic rich region and adjacent phosphocholine (POC) binding site, firm binding to the membrane (mainly driven by hydrophobic interactions) accompanied by the transfer of the N-terminal region to the lipid-water interface and finally pore formation after oligomerization of monomers..2. Incorporating a Cell attachment site, crucial for protein stability motif is critical for the successful design of the protein.3. The designed protein should be involved in cytolysis in another organism, cation transport by localizing to nematocyst, extracellular region, pore complex and exhibiting toxin activity.4. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "PQENTFYAGVKAGQASFHDGLRALAREYKVGYHRNSFTYGVFGGYQILNQNNLGLAVELGYDDFGRAKGREKGKTVVKHTNHGTHLSLKGSYEVLEGLDVYGKAGVALVRSDYKLYNENSSTLKKLGEHHRARASGLFAVGAEYAVLPELAVRLEYQWLTRVGKYRPQDKPNTALNYNPWIGSINAGISYRFGQS",
    "response#2": "PQENTFYAGVKAGQASFHDGLRALAREYKVGYHRNSFTYGVFGGYQILNQNNLGLAVELGYDDFGRAKGREKGKTVVKHTNHGTHLSLKGSYEVLEGLDVYGKAGVALVRSDYKLYNENSSTLKKLGEHHRARASGLFAVGAEYAVLPELAVRLEYQWLTRVGKYRPQDKPNTALNYNPWIGSINAGISYRFGQSPRDPCQCVCHGSAVTTQDCCPRQRGLAQLEVTFIQAWGLWGDWFTATDAYVKLFFGGQELRTSTVWDNNNPIWSVRLDFGDVLLATGGPLRLQVWDQDSGRDDDLLGTCDQAPKSGSHEVRCNLNL",
    "response#3": "DFMCLQNEVSEQTRLLAELSKDVQEVVFSSFLHMLCDRDVLYDLMKMLELNQLGHMDGPGGKILDELRKDSSLSWINLKDLILYLLQALMVLSDTQLCLLALSVEMRLLPHQVELVKSILQPNFKYPWNIPFTLQPQLLAPLQGEGLAITYELLEECGLKMELNNPPDRDNDGIPDSLEVEGYTVDVKNKRTFLSPWISNIHEKKGLTKYKSSPEKWSTASDPYSDFEKVTGRIDKNVSPEARHPLVSNARSECKRSHKFVPGAWLAGEGVDVTSLRRSGSFPVDTQRFLRPDGTCTLCENALQEGTLQRLPLALTNWRAQGSGCQRHVTRAKVSSTEAVARDAARSIRNDWKVGLDVTPKPTSNVHVSVAGSHSQAANFAAQKTHQDQYSFSTDTVECRFYSFHVVHTPPLHPDFKRALGDLPHHFNASTQPAYLRLISNYGTHFIRAVELGGRISALTALRTCELALEGLTDNEVEDCLTVEAQVNIGIHGSISAEAKACEEKKKKHKMTASFHQTYRERHSEVVGGHHTSINDLLFGIQAGPEQYSAWVNSLPGSPGLVDYTLEPLHVLLDSQDPRREALRRALSQYLTDRARWYRNGDPIPRPPNPQYSFSTDTVECRFYSFHVVHTPPLHPDFKRALGDLPHHFNASTQPAYLRLISNYGTHFIRAVELGGRISALTALRTCELALEGLTDNEVEDCLTVEAQVNIGIHGSISAEAKACEEKKKKHKMTASFHQTYRERHSEVVGGHHTSINDLLFGIQAGPEQYSAWVNSLPGSPGLVDYTLEPLHVLLDSQDPRREALRRALSQYL"
  },
  {
    "reference": "MLNTIEEAIEDIKAGKIIIVVDDEDRENEGDFLTAARNATPEVINFMAKHGRGLICAPITTDRCRELNLELMVGKNTATFETNFTVSIDLIGHGCTTGISASDRSKTIMALIDPKIKAEDLGRPGHIFPLISKNGGVIRRAGHTEAAVDLAELAGFEPAGVICEIMNEEGEMARLPELLEMAKKLDMKIVSIQDLITYRLRTESLIKKEVSVKLPTEWGDFEMVAFTQVNTGENHLALVKGSWEEDEPILVRVHSSCMTGDIFGSCRCDCGPQLHKAMEMIEQEGKGLIVYMNQEGRGIGLVNKLKSYNLQENGMDTFEANIALGFGMDERDYGVGAQIIRSLGVRKMKLMSNNPTKRAGLIGYGLEVVDNVAIEIESNKHNELYLKAKRDKMGHTIMKER",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The presence of GTP cyclohydrolase II domains is critical for the interaction of the designed protein with its intended targets.2. The protein design must be able to perform GTP binding, manganese ion binding, GTP cyclohydrolase II activity, magnesium ion binding in order to contribute to riboflavin biosynthetic process.3. The protein design should consider the endogenous proteins and enzymes present in the 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4, riboflavin biosynthesis pathway to avoid interference with native metabolic processes.4. The Nucleophile; for GTP cyclohydrolase activity, Proton acceptor; for GTP cyclohydrolase activity of the protein must be engineered to enable effective catalysis of GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.5. The protein should have a high affinity for GTP. The designed protein sequence is ",
    "response#1": "RMASASLPSEFGNFVMIGYESALSSDHHVAIVKGDIKNKKNVLVRVHSECMTGDIFGSYRCDCGVQLHHALDKIEEEGLGVVVYLRQEGRGIGLINKLKAYELQECGRDTVEANEDLGFSPDLRDYGIGAQILADLGLSSIRLMTNNPRKIVGLEGYGLEITEVVPDEEVLVDVKMPTEYGTFQLHAYKQKNTGEQHLALVKGAWEPGEPVLVRVHSSCVTGDIFGSHRCDCGEQLHNAMTMVEKEGKGVVLYMNQEGRGIGLEAKLRAYKLQEEGMDTVEANLHLGYKMDERDYGVGAQILRDLGISKLRLITNNPKKRAGLLGYGLEIVDERVTQAHMPTKYGNFKMVGYVNKVNGEHHIALVKGDITDGEPVLMRVHSECLTGDAFGSLRCDCGDQFAAAMKKINEEGRGLLLYMRQEGRGIGLINKIKAYALQDTGMDTVEANLALGFSEDMRDYGIGAEIIRDLGIKKIKLMTNNPKKINGLEGYGLEIVERVERVTQAHMPTKYGNFKMVGYVNKVNGEHHIALVKGDITDGEPVLMRVHSECLTGDAFGSLRCDCGDQFAAAMKKINEEGRGLLLYMRQEGRGIGLINKIKAYALQDTGMDTVEANLALGFSEDMRDYGIGAEIIRDLGIKKIKLMTNNPKKINGLEGYGLEIVERVEDVYDRVTEANMPTKYGDFKIVGYENKLNGEHHIALVKGNVSDGEPALVRVHSECLTGDAFGSLRCDCGEQYSAAMKKINDEGRGVLVYMRQEGRGIGLINKLKAYALQDKGMDTVEANLALGFPEDMRDYGIGAQILLDLGVQKIKLMTNNPKKLCGLSGYGIEIVDRV",
    "response#2": "RMASASLPSEFGNFVMIGYESALSSDHHVAIVKGDIKNKKNVLVRVHSECMTGDIFGSYRCDCGVQLHHALDKIEEEGLGVVVYLRQEGRGIGLINKLKAYELQECGRDTVEANEDLGFSPDLRDYGIGAQILADLGLSSIRLMTNNPRKIVGLEGYGLEITEVVPDEKVFEGADPLVDVKMPTEYGTFQLHAYKQKNTGEQHLALVKGAWEPGEPVLVRVHSSCVTGDIFGSHRCDCGEQLHNAMTMVEKEGKGVVLYMNQEGRGIGLEAKLRAYKLQEEGMDTVEANLHLGYKMDERDYGVGAQILRDLGISKLRLITNNPKKRAGLLGYGLEIVDRQAHAVLPSQFGAFQAIGYRNELXNSEHVALVKGDPASLQEPVLVRMHSECLTGDAXGSLRCDCRXQLEAALQRIEQXGEGVVVXLRQEGRGIGLINKLKAYSLQDGGLDTVEANXRLGFPADLRNYGVGAQXLSXLGXHRLRLLTNNPRKIAGLGGYGLQVEERVD",
    "response#3": "RMASASLPSEFGNFVMIGYESALSSDHHVAIVKGDIKNKKNVLVRVHSECMTGDIFGSYRCDCGVQLHHALDKIEEEGLGVVVYLRQEGRGIGLINKLKAYELQECGRDTVEANEDLGFSPDLRDYGIGAQILADLGLSSIRLMTNNPRKIVGLEGYGLEITEVVPVPPEAELIERVTQAHMPTKYGNFKMVGYVNKVNGEHHIALVKGDITDGEPVLMRVHSECLTGDAFGSLRCDCGDQFAAAMKKINEEGRGLLLYMRQEGRGIGLINKIKAYALQDTGMDTVEANLALGFSEDMRDYGIGAEIIRDLGIKKIKLMTNNPKKINGLEGYGLEIVERVERVTQAHMPTKYGNFKMVGYVNKVNGEHHIALVKGDITDGEPVLMRVHSECLTGDAFGSLRCDCGDQFAAAMKKINEEGRGLLLYMRQEGRGIGLINKIKAYALQDTGMDTVEANLALGFSEDMRDYGIGAEIIRDLGIKKIKLMTNNPKKINGLEGYGLEIVERVP"
  },
  {
    "reference": "MELIIASQNQNKLVEFKKILGDKINLFSLSDIGLNQEIPENEKTIKKNAMFKAKFVNTQTGKNVFADDTGLEIDSLNGEPGVYSARYSGLDRNSDKNIELVLRKLKNKANRNSRFKTIISLIIDGKSVNFEGVVEGKITEEKRGSNGFGYDPIFQPNGYSSTFGEMSLKEKNKISHRSIAINKMVQYLKEINLL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design must be able to perform ribonucleoside triphosphate phosphatase activity, dITP diphosphatase activity, metal ion binding, nucleotide binding, ITP diphosphatase activity in order to contribute to nucleotide metabolic process, purine nucleoside triphosphate catabolic process.2. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for dITP + H2O = dIMP + diphosphate + H(+).3. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.4. The protein should have a high affinity for Mg(2+), substrate.5. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions. The designed protein sequence is ",
    "response#1": "MKICFGTDGGGTKTVCALCDETGRKLGCYSSGVTNLSTAEQKNVMQEIFAELEEAGITADAVEYFVCATAGIANPEQREMLEGILATYGFRRAKVVNDGVAAVKGALGGRPGIVVISGTGSICFGRNQNGEEARAGGWGYLLGDEGSGYAIGRAGLKAACRAGDGRGRKTALVERVLEYMGATDARELVQRVYQAEIGKREIAALSRIVFECAMEKDPVALSIVEESARELAELAAAVIRKLHYGTDEIEVALVGSVFQSEVYIRAFEKALAEKYPGVKIIRPKYPPVVGAYYAALEKAGIPVTEAMVQKYTR",
    "response#2": "MRKIVLATGNKGKVRELQALLAPLAIEVTPQSAFNVSEAEEPHIAFVENALAKARHASRASGLPALADDSGICVDALGGAPGLYSARYAGEPKSDARNNAKLLAALVDQPAAQRTAHYHCVLVYLRHADDPTPIIAEGEWHGEILTAPRGEGGFGYDPLFLVPEQQCSAAELAPERKNALSHRGQAMRALLAKLQQ",
    "response#3": "MKKIVLATSNEGKYREIEEILIPLGYEIKSQSAFPHVLEVDEDGLTFRENAIKKAREVSKYTGYISLADDSGLEVEYLDGAPGVHSARYAGEHGTDEENNKKLLKALEGVEYEKRTARFRCVIALATPSGEILTVEGRVEGEILEKPRGENGFGYDPLFYLPEYGKTMAELSLEEKNKISHRGKALRKMKEILEKWFG"
  },
  {
    "reference": "MSKNVVIIGTQWGDEGKGKIVDLISDRAVSVVRFQGGHNAGHTLVIDQQTTVLHLIPSGILHDHVECLIAHGVVLSMSALLKEIAELEGANINVTERLKISPGCPLIFPYHIALDNAREAKCGKTAIGTTGNGIGPTYEDKVARRGLRAGDLLNPILFASKLKEVVEYHNFLLTNYYGTAPVDYQVILYEALSQAKKIKHMIIDVTEKIHQHIANDENILFEGAQGTLLDIDQGTYPFVTSSNTTSGGAVTGSGVGVTDINYVLGIVKAYTTRVGSGPFPTELIYNVSTNKGDAIGKVLGTVGHEFGATTGRQRRCGWLDMVTLKRSFNLNAVTGICLTKLDVLDSLISVKICVAYKLNGKEVTIPPYDAKGYTDAKPVYIEMPGWKTSTIGTNSFDSLPIEAQNYIHKIEQLANLPVDILSTGPDRTQTLILKHPFE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.2. The designed protein should have a high specificity for substrate, Mg(2+) binding, with minimal off-target effects.3. The protein must have high specificity towards magnesium ion binding.4. A protein that can modulate the overall flux of metabolites through the AMP biosynthesis via de novo pathway, Purine metabolism, AMP from IMP: step 1/2 pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "QPYRGLKGALMEMVNLAEGVVRDGEVRSGDGRGARELQDGICGLQGLQDGALSTWRRQGSKSGALEAKEVGDRINAAYAAARNVLDRPALLTDVWFLYTPSQMTFAALYLADAPLTDFYLGTKLPMDSEARPKILAVIHSCAELL",
    "response#2": "QPYRGLKGALMEMVNLAEGVVRDGEVRSGDGRGARELQDGICGLQGLQDGALSTWRRQGSKSGALEAKEVGDRINAAYAAARNVLDRPALLTDVWFLYTPSQMTFAALYLADAPLTDFYLGTKLPMDSEARPKILAVIHSCAELL",
    "response#3": "QPYRGLKGALMEMVNLAEGVVRDGEVRSGDGRGARELQDGICGLQGLQDGALSTWRRQGSKSGALEAKEVGDRINAAYAAARNVLDRPALLTDVWFLYTPSQMTFAALYLADAPLTDFYLGTKLPMDSEARPKILAVIHSCAELI"
  },
  {
    "reference": "MTRNERIFHAVLFELMALAIIVPAAALITGKGSSDLALVGIGLSLYTVVWNYIYNLYFDKWFGSNRADRSLAMRLGHTVGFEGGLIFISIPVIAWFLEITFLRALMLEAGFLVFFLFYATGFNWLYDKVQPFGKMRKLLV",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Confers resistance to chlorhexidine, benzalkonium, proflavine and acriflavine; Mediates efflux of both proflavine and acriflavine via an energy-dependent mechanism.2. The designed protein should be expressed specifically in the plasma membrane The designed protein sequence is ",
    "response#1": "VLVGLVLAIFLASLDFTIISTAIPKITDEFHSLQDIGWYGSAFFITTAATTAHWGRLYTFFPLKWTYLTSIFFFELGSVICGAAPSSTALIIGRAICGIGAAGLFSGSYTIIAVLVPSADRPKYSGLMGASYGLASVVGPLIGGAFTSHVSWRWCFYINLPVGGVSCLFILLFFANEPPKSRPDWRGMLRQLDMLGLVLIVGAITCFILALQWGGVSKAWDSGAVIGTLVAFVVCMILFVIEQWWLGENAMVHSVMIKRRTIWVGSMFSFLINSAFLVTFYYLPIYFQSVQGVSASTSGVRTVPFILAVTFCVVIVGQIITKTGYAFPWMIVGAAITTIGSGMIYTFDTHSPAGKWIGYQILCGIGVGVSFQVPVMLIQATTKDADVPLATATLLFIQTLGGAFGVSSAQAAFQNTLLKQLAITAPGLNPQIVLDAGASELKRVIPEQFLQGVLEAYVSGFRECLIVGIAFGGAAFLAAFGFRFTNIKRTAAAEV",
    "response#2": "NSFAVFVPPVLMRSLLEYLEVPDLASQSTAWLYVTGLLVAGIVAGVAGCQCDWKGREMAARTRAVLINEIYTKVLRKGVALHLQTNSEQPEAADNFASDGNIFNLLTVDTEHVSEMSGYLYLVWITFPVQTAIGTYLLYRLLGISGIVGVALMLGLLPLNILISRRLVAVQARVLTASDARIQASSEILNNVRTIKYSAWEAVFKKRVLSKRRIELVEMRSRFIWWSINMTTFFSLPLIVTILTLFFYTVVWDNSMGTAVAFPALL",
    "response#3": "NSFAVFVPPVLMRSLLEYLEVPDLASQSTAWLYVTGLLVAGIVAGVAGCQCDWKGREMAARTRAVLINEIYTKVLRKGVALHLQTNSEQPEAADNFASDGNIFNLLTVDTEHVSEMSGYLYLVWITFPVQTAIGTYLLYRLLGISGIVGVALMLGLLPLNILISRRLVAVQARVLTASDARIQASSEILNNVRTIKYSAWEAVFKKRVLSKRRIELVEMRSRFIWWSINMTTFFSLPLIVTILTLFFYTVVWDNSMGTAVAFPALL"
  },
  {
    "reference": "MLLAWVHTFLLSNMLLAEAYGSGGCFWDNGHLYREDQPSPAPGLRCLNWLAAQGSRESLTEPSPGNHNYCRNPDQDPRGPWCYISSETGVPEKRPCEDVSCPETTSQAPPPSSAMELEEKSGAPGDKEAQVFPPANALPARSEAAEVQPVIGISQLVRMNSKEKKDLGTLGYVLGITMMVIILAIGAGIIVGYTYKRGKDLKEQHEKKACEREMQRITLPLSAFTNPTCETVDENTIIVHSNQTPADVQEGSTLLTGQAGTPGA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein localizes to the plasma membrane.2. The protein must contain a signal peptide for proper functionality.3. The Extracellular, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid. The designed protein sequence is ",
    "response#1": "MPGTIGGLALRVGQFLFAAAAIVIMVTGDEFTNYTAFCYLVAAMSLQFLWSFMLAILDTYALLIKRGLRNSVLLSLFVVGDWVTATLSLAAACSTAGVTVLFDNDLNYCGQMHCHRYQLSAAMAFLSWLL",
    "response#2": "MPGTIGGLALRVGQFLFAAAAIVIMVTGDEFTNYTAFCYLVAAMSLQFLWSFMLAILDTYALLIKRGLRNSVLLSLFVVGDWVTATLSLAAACSTAGVTVLFDNDLNYCGQMHCHRYQLSAAMAFLSWLI",
    "response#3": "MPGTIGGLALRVGQFLFAAAAIVIMVTGDEFTNYTAFCYLVAAMSLQFLWSFMLAILDTYALLIKRGLRNSVLLSLFVVGDWVTATLSLAAACSTAGVTVLFDNDLNYCGQMHCHRYQLSAAMAFLSWLM"
  },
  {
    "reference": "MGGKWSKSSLVGWPAVRERMRRTEPAADGVGAASRDLEKYGAITSSNTANTNAACAWLEAQEEEGVGFPVRPQVPLRPMTYKGAVDLSHFLKEKGGLEGLIYSQKRQDILDLWVYNTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEKEKVEEANXGENNCLLHPMALHGMDDPEGEVLMWKFDSRLAFHHVARELHPDYYQNC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit GTP binding.2. The protein must exhibit the following characteristics: Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis. The designed protein sequence is ",
    "response#1": "MERPNSSVAGLYSGLHGNGSVENLATEEEGLRLLAGAASARFGSSAGRGGGGC",
    "response#2": "MERPNSSVAGLYSGLHGNGSVENLATEEEGLRLLAGAASARFGSSAGRGGGGA",
    "response#3": "MPGSN"
  },
  {
    "reference": "MSVDLQTVKRVARLARIAVSEEDAERMTGELNAILGFVEQLNEVDVSGVEPMTSVTPMEMKKRQDVVTDGNKAADIVANAPATEENFFLVPKVVE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein with glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity, ATP binding is needed to enhance regulation of translational fidelity in a specific context.2. The protein should have a fast turnover rate for ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+)   + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate.3. The protein must exhibit the following characteristics: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl- tRNA or glutaminyl-tRNA synthetases. The designed protein sequence is ",
    "response#1": "IVVGLEVHAQVVSEAKLFSGASAEYGGEPNTHVSLVDAGFPGMLPVLNRECVAQAVRTGLGLEAEINLFSRFDRKNYFYADLPTGYQISQFAHPIIGKGKVLVDLADGSTREIGVTRLHLEQDAGKSIHDQDPTRSFIDLNRAGVALMEIVSEPDIRSPEEAGAYLRKLRQILRYIGTCDGNMEEGSMRADVNVSVRKPGETEYRTRCEIKNVNSIRFVMMAIEVEAQRQIEVWEAGGTVDQETRLFDHVRGETRSLRSKEDAHDYRYFPDPDLLPLVIEQSWIEEIKKDLPEMPEEKKARYVKEYGLPEYDAGVLTASKEMADYFEACLEFHSQPKPVANWVMGEVTRALNEENRSITDAPVTPQLLAELLQLIDKGTISGKIAKTVFDEMWRTGKAPAKVVEEKGLVQVSDTGEIEKIIDEVLAREAGQVAEYRSGKDKLFGFFVGQVMRASKGKANPAVVNELLLKKLGVEG",
    "response#2": "TVIGLEIHAQLATRTKIFSGAPTAYGAEPNTQACPVDLGLPGVLPVLNREVVRMAIKFGLAVDARIAPRSVFARKNYFYPDLPKGYQISQYDLPIVEGGHLDIELEDGASKRIGITRAHLEEDAGKSLHEDFHGMTGVDLNRAGTPLMEIVSEPDLRSPAEAAAYMKKLHALVRYLEICDGNMQEGSFRCDANVSVRPVGQEAFGTRAELKNLNSFRFVERALEYEVERQIDLLESGGEVVQETRLYDVDKGVTRSMRTKEEANDYRYFPEPDLLPVEVDAALAERVAEIPELPFEARRRFVGEWGLSAYDAGVLTSSREMADFFEEAVKDGGDAKKVANYLMNDVNSYLNNQHVDLDDTKLTPANLAGMIKLIDDGTISSKMAKKVFQGILDGQEPAAFAKANGLVQLSDPAELQPIIDAILDDNEQSIEDFKNGKDRAFGFLIGQIMKKTQGKANPKVVNQLLGKSL",
    "response#3": "MSYEVVRSYLERIDAAREDNIFITVFHERALERARMLDRKLAEGGTVGKLFGLPMAIKDNIAMKGERLTCASKILENYESVFDATVILRLEAEDAIFLGKTNMDEFAMGSSNENSAFGNVPNPFDKSRVPGGSSGGSAAAVAANLALVALGSDTGGSVRQPAGFCDIVGLKPTYGRISRFGLVAFASSFDQIGVLARTCGDAALVLEVMAGKDERDATSSAHQVDSYHSMMERVSPEGLKIGVPQEFFTDALNADVARLVLATLDDLRNRGAELVDITLPDSAYAIAAYYILVTAEASSNLARFDGARYGFRTSEAADLAAMYVNSRTEGFGREVKRRIMLGTYVLSAGYYDTYYKKAQQVRRYFQDQYRAALQHVDVIAGPTSPFPPFGLGDKTGDPLEMYLADVFTVPASIVGMPAVSVPLGFDSQKLPVGMHLVGNFFEEGKLLAAVDFMEKDFFEEAVKDGGDAKKVANYLMNDVNSYLNNQHVDLDDTKLTPANLAGMIKLIDDGTISSKMAKKVFQGILDGQEPAAFAKANGLVQLSDPAELQPIIDAILDDNEQSIEDFKNGKDRAFGFLIGQIMKKTQGKANPKVVNQLLGKSV"
  },
  {
    "reference": "MISQHPSWQRRRGRGRWREASSWRRKLPEQRWWKRRFWSDKMISPKDVSILDINSGECGVPPSVLMGNAGRALAEIISREHSGGSVLFVLGTGNNGGDGLVAARHLSEGGHPVSVLLAREPARIRSTIARKAFHSLPVNVRVLVMESMEKEDLERTFESAEIIVDGLLGSGASGEPRGVYRDAVEMMNASGRPVISIDIPTGVGTPVHVNARRTVTFHDVKDSMVVDGTPRPECGELEIAEIGIPREASLYVGRGDLRRIPVKGPETHKGEGGKLLIVGGGPFTGAVILASLAAVKMGVDLVRVCVPRGIWEVVASASPDLIVSRLDTEDPFQLDPGVIDDMDAELEWADAVLMGPGTGRSDETVEFLRKLFISCGERGLDVVVDADGLKAISPLMQGSVRGEGCILLTPHRGELRTLLDGAGIEYDPEVLLHPLIEIEGCMQWSDEAMAAVRALIERTGCDILVKGPLDMIASPSPEHSLSDHIRRDGLLTRYNRTGVPEMSVGGTGDVLSGLCAGMMARGMSSFDSACVSAYINGRCGELAREELGTSLSASSLLERISRILQGGISFSPSASG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have a binding site that is specific to the target K(+), AMP ligand.2. A protein with ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, metal ion binding, NADHX epimerase activity functions is required to enable nicotinamide nucleotide metabolic process.3. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.4. The desired protein should be able to perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate The designed protein sequence is ",
    "response#1": "MLRPLYTADEMRAAEQGHDVEAMMERAGSAVAETVLRRYPDARRIAAVCGKGANGGDGRIALRVLAEAGRETGEELDGADVVIDALFGTGFHGAPRDDAAGRIEQINGAGVPIVAVDLPSGVDASTGEVVGAVVDADVTVTMHGPKVGLEVGPGRFHAGEVEVAEIGLDDATLGALLPVRTASAHKGTAGRVWIVGGHPGTVGAPALAGLGALRAGAGLVTVHSAADVPLVTPELMAQRHADLRTFLTSTGLTGTGARPDAVAIGMGLGPEAPALAREVLDWEIPTVLDADALQPDLCGYGHDRCVWTPHPGEAARVLGVPTPEVTRDPLSAARALQDRLGGVVVLKGGPSVVARAGDLSVARGGHPGMASAGMGDTLCGILAALLAQGLDPWDAARAGVRLHARAGEIAAVRFGYGLGATDVAHDLGRAWADFLTAESIALLLRRRDRQAHKGHFGHCLIVAGSSGKTGAAALSANSAVRAGSGLVTLAVPESLNPILEVKTTEAMTVPLADSGGGYLTDSAFPAIAGLLPDKDALAIGPGLGQHPATVTLVRALLKAADLPLVIDADGLNAVAQNANCLLDKKSTHVVLTPHPGEMARLMGATIADVEADRIATARKFAAAFGVHLILKGSRTIIAAPDGSTAINGSGNPGMASGGMGDVLTGIIVSLLGQGYNCHDACRLGVFIHGYAADLVAQDKGEIGMCAGDVQEKLPYVWIVGGHPGTVGAPALAGLGALRAGAGLVTVHSAADVPLVTPELMAQRHADLRTFLTSTGLTGTGARPDAVAIGMGLGPEAPALAREVLDWEIPTVLDADALQPDLCGYGHDRCVWTPHPGEAARVLGVPTPEVTRDPLSAARALQDRLGGVVVLKGGPSVVARAGDLSVARGGHPGMASAGMGDTLCGILAALLAQGLDPWDAARAGVRLHARAGEIAAVRFGYGLGATDVA",
    "response#2": "MVVLDGQQVVSFEKSCGVAIDELICKAGKVIGDTIFNMFPRQPVIVISGPGNNGRDGIVAAKMLKAHGWPVVLMLYNCTTHIDEDWVVPLTYDNIVNSQSCLIVDALFGIGLSRYIPEDLSCIFSYINNSNNKIVVAVDIPSGINCNTGQVMGCAIRADVTITFSALKIGHVLFPGSSYSGKVRVVDIGIGIDYSEPALWLPEYPWPTVEGHKYLRGHAVVASGPIYSTGAARLAARGALRAGAGLVTVASPRDALAVNAAQLTAIMVREADDARGLAALLADTRKNAVLIGPGHGVGEGTRAMVLAALAAGPRVVLDADALTSFADDPETLFKAIHASEAPVVMTPHEGEFARLFGDLIAEPKVERTRKAAARAGATVLLKGADTVIAAADGQAAINGTASPWLATAGSGDVLGGIVLGLLAQGMTGFLGASAAVWMHGEAAKTFGPGLIAEDLPEMLPAVLRGILQEG",
    "response#3": "MRALDVNAEYFGISRLQLMENAGGSVAAEIALRFKPKETRVAVFCGLGGNGGDGFVAARHLACLGFNVNVILAGKSSEISVEESRRNWAALKHLRRSVTIYEVHDSSLIPNIEADVIVDALLGIGLKAPPRPPIAQLIRAINDSDAFRVAVDVPSGLDSDTGAVLGEAVKANLTVTFHKAKPGLLKAKEYVGELIVRQIGIPREAEGWLETVLVVGGAPGLTGAVCLAAEAAFRADAGYVAVATPPASFPVIEARLLEAVKAPLQRVDELAERATALAIGPGLGRGEEERALVRRLLAERTMPAVVDADALFELEPADWPAPRVLTPHSGELGRLLGEESRWVDAHRLEAVRRAADRYRCVVLLKGADTLVAAPGEGVLVSNSGTPALATAGTGDVLTGVVAAFLAKGVDARLAAAAAAFVHGRAAELVPHERGVVAVLVVGGAPGLTGAVCLAAEAAFRADAGYVAVATPPASFPVIEARLLEAVKAPLQRVDELAERATALAIGPGLGRGEEERALVRRLLAERTMPAVVDADALFELEPADWPAPRVLTPHSGELGRLLGEESRWVDAHRLEAVRRAADRYRCVVLLKGADTLVAAPGEGVLVSNSGTPALATAGTGDVLTGVVAAFLAKGVDARLAAAAAAFVHGRAAELVPHERGVVAVLCVGGDTGHGGAVMLAADAALRCGAGLASVATRSAHVPGLLARRPEAMARGVDGAAALAPLLARADVVALGPGLGQGSWGRALYGAVLAAGLPLVLDADALNLLAAAPRELAADTILTPHPGEAARLLGMETTQVQADRFAAAARLVERYGCVVVLKGAGTVVAAPGQSPRVVAAGNPGMAVGGMGDLLTGCIAALRARGMPAFEAASLGALLHALAGDAAARDGETGLLPSDL"
  },
  {
    "reference": "MTGRAVRTLVLGGVRSGKSAYGESLLGSHRQVRYLATGSRTSTDAEWLARIAAHRERRDDRHITIETTDLAGELRAAPDIPVIVDDLGSWLTARVDALGGWESGDVDLDPEIADLCDALRGMNADTVVISSEVGLSLVSSTPAGRLFQDLLGTLNAAVAQACDRVSLVIAGRVLDLPRGEFDASPVPPTAAEQAPNDRTSNDQTSNNQAEEAPANAPETAIAATPAPGTAGPPAARTRVAATPVAADASDLPDPTDAEIFPAVTPPDEAVAFTARERQNSLTKPPGSLGRLEEIGVWIAACQGQCPPAPIVKPAVVVFAGDHGVARGGVSAFPPEVTAQMVANIEHGGAAVNVLAARAGASVTVVDMSVDADTAPHLSTYKVRRSSEDLRTTDAITIAEARAALAAGRAIADRLVDSGSDLLIAGEMGIGNTTPATVLVGALTRREPVEIVGRGTGVDDHGWMRKTAAIRDGMRRARKVVHDPLALLAAVGGADLTAVAGFLAQAAVRRTPVILDGLVITAAAMVADALAPGATRWWIAGHRSVEPAHQIALDHLDLKPVLEMSMRLGEGSGAVLALPIVQSAVDIMTSMATFDEAGVAGRDDSPADVEIP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. We require a protein that can specifically bind to the metabolites produced in the alpha-ribazole biosynthesis, Nucleoside biosynthesis, alpha- ribazole from 5,6-dimethylbenzimidazole: step 1/2 pathway.2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.3. The protein design must be able to perform adenosylcobinamide kinase (GTP-specific) activity, nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity, ATP binding, adenosylcobinamide kinase (ATP-specific) activity in order to contribute to cobalamin biosynthetic process.4. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity.5. The protein must exhibit the following characteristics: Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB).. The designed protein sequence is ",
    "response#1": "MTSLSTNLQNLNDIPLASALKNITIKEISGDTDIKIKGVXNDSRKVMPGDGFIAIKGYLFDGHKFVTDAISQGAKVIIAEKELDVDLPFIQVKDTLKSLSLXASXIYXNPSKKLNXIG",
    "response#2": "MTLLAXDXSLXGDXLXRXAXTXGXXHXLXXGXVXGTAXDXSXXRXLPXAALX",
    "response#3": "MREAXSRVXXSXLXLPXXRXVXPXXRXRXVXGXDEXSXSXDXXRXRXPX"
  },
  {
    "reference": "MEYLPLFAQLKQRPVLVVGGGEVALRKIALLRRAGACVSVVAKKLHPELAALEQEGALRWLAQSFEPAQLDAVFLVIAATSDAALNRRVFDEANARQRLVNVVDDQPLCSFIFPSIVDRSPLIVAISSSGNAPVLARLLREKIESLLPASLGRMAEVAGGFRDRIKARIPTTDGRRRFWEKAFRGRFASLMAAGDTRAAEAVLEAELDAPQGPQGEIILVGAGPGDAGLLTLRGLQVMQQADVVLYDHLVSDGVLDLVRRDADRICVGKRAGAHAVAQHETNQMLIDFAREGKTVVRLKGGDPFIFGRGGEELEAAKAAGVPFQVVPGVTAASGATAYAGIPLTHRDYAQSAVFVTGHYKPDSEPFDWALLAKSRQTLAIYMGTMKAAEISQQLIAHGRDATTPVAVISRGTREDQRVLTGTLDTLNILAKDAPMPALLVVGEVVQLHQQLAWFQHSTDAENLRSSVVNLA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein with sirohydrochlorin ferrochelatase activity is needed to enhance siroheme biosynthetic process, methylation in a specific context.2. We require a protein that can specifically bind to the metabolites produced in the Porphyrin-containing compound metabolism pathway.3. The designed protein should have a high specificity for S-adenosyl-L-methionine binding, with minimal off-target effects.4. The Proton donor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "VGAGPGDPGLLTLRGLRVLNEADVILHDRLVSDEVLALARRDADRIELGKQVGGDHEATQARIHALLVEHARAGKRVVRLKGGDPFVFGRGGEELEVLHAHGIDFEVVPGITAAVACAAYAGVPLTHREHAQSVRFVTALGRDGTDPVDWPALARERQTLAFYMGVSGLTRLREQLIAHGRAPGTPFALVENGTRADQRVVTGTLDTLPEAAARHAVKAPALLILGEVL",
    "response#2": "VGAGPGDAGLLTLNGLQQLQQADVVVYDRLVSQEVLAHVRRDAERIFVGKEAGCHCVPQQAINQLLLEQARLGKQVVRLKGGDPFIFGRGGEELETLAEAGIPFSVVPGITAASGCAAYSGIPLTHRDHAQRVQFITGHDKEGNIAQEWPALATPRQTLVFYMGLAHAGRIQDELQTHGLPGHTPVALVEQGTRLQQRVVRGKLQQLAQLATQVESPSLIIIGSVPF",
    "response#3": "GEVYLVGGGPGDPDLLTFRALRLMQKADIVLYDRLVSKEVLALVRRDAELVYVGKTAGDHPVTQDNINEKLAEYALQGKRVLRLKGGDPFIFGRGGEEISHLAEYGIPFQVVPGITAASGCASYAGIPLTHRDYAQSVRFITGHLKDNSLDLNWQDLVSPNQTLVFYMGLSGLESICRELIRRGKAAGTPAALIEKGTTANQRVFIGDLKTLPDKVREANAKAPTLIIVGDVVKLE"
  },
  {
    "reference": "MSSGQQPPRRVTNVGSLLLTPQENESLFSFLGKKCVTMSSAVVQLYAADRNCMWAKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDAPNGPNLPMATVDIKNPEITTNRFYGSQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHSSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPGVVVPPPPPNRMYPPPPPALPSSAPSGPPPPPPPSMAGSTAPPPPPPPPPPPGPPPPPGLPSDGDHQVPAPSGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVSDGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDDDDEEDFEDDDEWED",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should exhibit Acidic residues, Polar residues compositional bias.2. For general function, the protein need meet that Plays a role in dendrite spine morphogenesis.; Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia; In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization ; Regulates actin polymerization by stimulating the actin- nucleating activity of the Arp2/3 complex; Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression; Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization3. The designed protein should contain one or more WH1, CRIB, WH2 domains that are essential for its function.4. The designed protein should be involved in regulation of postsynapse organization, actin filament-based movement, vesicle transport along actin filament, actin filament polymerization by localizing to cell leading edge, lamellipodium, cytosol, postsynapse and exhibiting identical protein binding, actin binding, cytoskeletal protein binding. The designed protein sequence is ",
    "response#1": "VGVSDLTLISKVSNEAINENLKKRFDNREIYTYIGHVLVSVNPFRDLGIYTDAVLESYKGKNRLEMPPHVFAVAESAYYNMNAYKDNQCVIISGESGAGKTEAAKRIMQYIANVSGGSNSSIQETKEMVLATNPLLESFGNAKTLRNNNSSRFGKYLQLQFNAQGEPVGADITNYLLEKTRVVTQIKDERNFHIFYQFTKGASQAYRESYGIQQPSQYLYTSKAGCFDVDGIDDLAEYQDTLQAMKVIGLSQAEQDEIFRMLAAILWTGNIQFREGDDGYATVVDQSVVDFLAYLLDVDAAHVIQAITIRILTPRNGEVIESPANVPQAMATRDALAKAIYNNLFDWIVERVNKSLTARAETSNSIGILDIYGFEIFEQNSFEQLCINYVNEKLQQIFIQLTLKTEQEEYAREQIKWTPIKYFDNKIVCDLIEAIRPPGVFSAMKDATKTAHADPAACDRTFMQAISGMSNPHLTPRQGNFIIKHYAGDVSYTVEGITDKNKDQLLKGLLNLFGQSRNHFIHELFPHQVDQDNRKQPPSAGDKIKASANDLVTTLMKATPSYIRTIKPNENKSPTEYNEKNVLHQVKYLGLQENVRIRRAGFAYRQTFDKFVERFYLLSPKTSYAGDYIWTGDSKTGAMQILKDTNIPVEEYQMGVTKAFIKAPETLFALEHMRTTKKKEVGVSDLTLISKVSNEAINENLKKRFDNREIYTYIGHVLVSVNPFRDLGIYTDAVLESYKGKNRLEMPPHVFAVAESAYYNMNAYKDNQCVIISGESGAGKTEAAKRIMQYIANVSGGSNSSIQETKEMVLATNPLLESFGNAKTLRNNNSSRFGKYLQLQFNAQGEPVGADITNYLLEKTRVVTQIKDERNFHIFYQFTKGASQAYRESYGIQQPSQYLYTSKAGCFDVDGIDDLAEYQDTLQAMKVIGLSQAEQDEIFRMLAAILWTGNIQFREGDDGYATVVDQSVVDFLAYLLDVDAAHVIQAITIRILTPRNGEVIESPANVPQAMATRDALAKAIYNNLFDWIVERVNKSLTARAETSNSIGILDIYGFEIFEQNSFEQLCINYVNEKLQQIFIQLTLKTEQEEYAREQIKWTPIKYFDNKIVCDLIEAIRPPGVFSAMKDATKTAHADPAACDRTFMQAISGMSNPHLTPRQGNFIIKHYAGDVSYTVEGITDKNKDQLLKGLLNLFGQSRNHFIHELFPHQVDQDNRKQPPSAGDKIKASANDLVTTLMKATPSYIRTIKPNENKSPTEYNEKNVLHQVKYLGLQENVRIRRAGFAYRQTFDKFVERFYLLSPKTSYAGDYIWTGDSKTGAMQILKDTNIPVEEYQMGVTKAFIKAPETLFALEHMRD",
    "response#2": "VGVSDLTLISKVSNEAINENLKKRFDNREIYTYIGHVLVSVNPFRDLGIYTDAVLESYKGKNRLEMPPHVFAVAESAYYNMNAYKDNQCVIISGESGAGKTEAAKRIMQYIANVSGGSNSSIQETKEMVLATNPLLESFGNAKTLRNNNSSRFGKYLQLQFNAQGEPVGADITNYLLEKTRVVTQIKDERNFHIFYQFTKGASQAYRESYGIQQPSQYLYTSKAGCFDVDGIDDLAEYQDTLQAMKVIGLSQAEQDEIFRMLAAILWTGNIQFREGDDGYATVVDQSVVDFLAYLLDVDAAHVIQAITIRILTPRNGEVIESPANVPQAMATRDALAKAIYNNLFDWIVERVNKSLTARAETSNSIGILDIYGFEIFEQNSFEQLCINYVNEKLQQIFIQLTLKTEQEEYAREQIKWTPIKYFDNKIVCDLIEAIRPPGVFSAMKDATKTAHADPAACDRTFMQAISGMSNPHLTPRQGNFIIKHYAGDVSYTVEGITDKNKDQLLKGLLNLFGQSRNHFIHELFPHQVDQDNRKQPPSAGDKIKASANDLVTTLMKATPSYIRTIKPNENKSPTEYNEKNVLHQVKYLGLQENVRIRRAGFAYRQTFDKFVERFYLLSPKTSYAGDYIWTGDSKTGAMQILKDTNIPVEEYQMGVTKAFIKAPETLFALEHMREQ",
    "response#3": "LSLEEVLKSYEQPINEEQAWAVCFQCCGDLRAPRPQPHPRRPELQIRDPASIILHRDGRVTALLQSCDDSNAGCESAADGKLVQSLGVAIYRALDWGLDDSEERELSPQLEQLIEFMVGGPDCGQSKHCGSNAAKDEGYSGQDEEEEEEEEEEEEGAGRGIHTVQQVMTMCASRLANPVLAPEHYQAVCRALFLETLESSEEGLLLWCKNTTTGYDGVNITSFTKSFRDGLAFLALSHKFEPESFKFQEFEAMDPIARLNAAFDFAEKGLGVPKLLEAEEVMRGTTDERSLVLYTSLFFHAYHHGLPQAAYKDVLDEKTKTDWALYTYEGNSNDIRLAETGDGGLEELVEELSSGKVMYAFCRVKDPNSGLPKFVLINWTGEGVKDARKGMCANHVSTMASFLKGAHVTINARAEEDVEPESIMEIPKSTNKIIAAAVVKLYVAYPDPNKWNYTGLCGALVLSYDTTAKCCWFKLVDVVNNSGIIWDQELYQNMDYRQDRTFFHSFELDKCLAGFSFANETDAQKFYKKVLDKGCHSLIADELKNGPALTAAYNEVVGEKSETNWVLFTYEGNTNDIRLAEKGDGGLEEMVEELNSGKVMYGFCRVKDPNSGLPKYVLINWTGEGVKDARKGQCANHVSSMANFLKGAHVTINARAEEDVEPDAVMEKVPKSTNKIIAAAVVKLYVAYPDPNKWNYTGLCGALVLSYDTTAKCCWFKLVDVVNNSGIIWDQELYQNMDYRQDRTFFHSFELDKCLAGFSFANETDAQKFYKKVLDK"
  },
  {
    "reference": "MLNRVVLVGRLTRDPELRYTPNGVAVANFTLAVNRPFSNQQGEREADFINCVVWRKQAENVANYLKKGSLAGVDGRIQTRSYDNNEGRRVFVTEVMAESVQFLEPRGSQSQGGSNVDNFGGGSPNNPMGGNDFGQQSGGSGRQSGGFSEDPFANDGKPIDISDDDLPF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should exhibit Polar residues compositional bias.2. The designed protein must possess single-stranded DNA binding to support DNA replication, DNA repair under certain conditions.3. The protein's functional design necessitates the inclusion of a Important for interaction with partner proteins motif. The designed protein sequence is ",
    "response#1": "IAQVRDEGLGMTDDTDWFSIKATIIYVKQDNFAYPACRTTDPQPCNKKVIENEPGNWRCEKCDKSWDAPKYRYIMSVNVSDHTGQIWLSCFDEVGERIMGMPANDLMAMKEEGDDKRVTDAFGDANCQTFNFRVKAKMDNFQDQQRVRYQVQYANPLDFSREATKLAQIIKS",
    "response#2": "SSCKPLDELLGGGFESGVVTQIFGEAGSGKTNICLQLAIECVKKGKKAIFIDTEGLSADRFKQIAGENARKIAQDIIIFEPHSFEEQYSAVRETEKISTENVGVIIVDSATAYYRFELDDEDSSIRTRRELSNQIGFLHSLARKRDIVVVITNQVYSDIKSNSLKPIGGSSLEHISKTIIQLEKTGTGSRRAKIWKHRSRPEGTTCEFTITADGC",
    "response#3": "IAQVRDEGLGMTDDTDWFSIKATIIYVKQDNFAYPACRTTDPQPCNKKVIENEPGNWRCEKCDKSWDAPKYRYIMSVNVSDHTGQIWLSCFDEVGERIMGMPANDLMAMKEEGDDKRVTDAFGDANCQTFNFRVKAKMDNFQDQQRVRYQVQYANPLDFSREATKLAQIIKK"
  },
  {
    "reference": "MKVKVFNLEGEPVEEIELPKVFSTPFRPDLIRRAVIASWTHRIQPQGRDPLAGKRRVTENIGKGHGMARVERIKTPPRFAAFVPFARGGRRTHPPKVEKIIWEDINKKERRLAIMSAIAATANPDLVRARGHVVDNVPAFPLVVVDDLQKVFKTAQTREIFKKLGIWDDIERAKRNTKIRAGKGKMRGRRYKKAKGPLIVVAKNEGIVQGARNHPGVDVVTVDNLGVELLAPGTHPGRLTIWTKGAIERLKEIYG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have a specific function within the ribosome. The designed protein sequence is ",
    "response#1": "EIDVHVPIHLVGEAAGVKDGGVLQQAMHQLSIRVLPSNIPPSIDVDITNLQVGDVITVSHIQTNGAYEINHDPTEVIASILPPS",
    "response#2": "EIDVHVPIHLVGEAAGVKDGGVLQQAMHQLSIRVLPSNIPPSIDVDITNLQVGDVITVSHIQTNGAYEINHDPTEVIASILPPS",
    "response#3": "EIDVHVPIHLVGEAAGVKDGGVLQQAMHQLSIRVLPSNIPPSIDVDITNLQVGDVITVSHIQTNGAYEINHDPTEVIASILPPS"
  },
  {
    "reference": "MCTGRRGGWMRGERHGSARRGGAWGGGGGGGRGGGGRAGLTPCPAPPPAPVGSRVGLRSPVMASKLLRAVVLGPPGSGKGTMCEKIARSFGLQHLSSGQFLRENLGGEVGVLAKQYLERGLLVPDHVITRVMMSELEKRRDQPWLLDGFPRTLGQAEALDRICELDLVISLNIPFETLKDRLSARWVHPASGRVYNMDFNPPHVQGIDDLTGEPLVQREDDKPEAVAARLRKYKDAAKPVIELYKSRGILHSFSGTETNKIWPYVYTLLSSKIPPILSDEEN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit adenylate kinase activity. The designed protein sequence is ",
    "response#1": "GFEPHPALMNAVFLLACHFARSPYCTEIEPVFFSRTLHEITVALDNSDRLVDVVQASCLLAVYLYINCRSLEGYCHSFSAARLAVGLGMHQIKRFLPLDGMGGASTEDITPIPIPPPRDRAEVQDRISAFWQVFMVDRCWSVASGLPVALPE",
    "response#2": "EFCEIFRAKDKNTLKMYTCKKFLKKDGRKVRKAAKNEIVILKMVKHPNILQLVDVYETRKEYYLFLELATGREVFDWILDQGYYSERDTSNVIRQVMEAVAYLHSLKIVHRNLKLENLVYYNRLKHSKIVISDFHLAKLENGLIKEPCGTPEYLAPEVVGRQRYGRPVDCWALGVIMYILLSGNPPFYDEADDDDYENHDKNLFRKILAGDYEFDSPYWDEISDSAKNLVSRLMEVDQDQRLTAQEAINHEWL",
    "response#3": "GFEPHPALMNAVFLLACHFARSPYCTEIEPVFFSRTLHEITVALDNSDRLVDVVQASCLLAVYLYINCRSLEGYCHSFSAARLAVGLGMHQIKRFLPLDGMGGASTEDITPIPIPPPRDRAEVQDRISAFWQVFMVDRCWSVASGLPVALPP"
  },
  {
    "reference": "MLQIYNTLSRSKQVFKPIVPGKVKMYVCGMTVYDFCHIGHARVMIVFDMVVRWLRASGYEVQYVRNITDIDDKIIKRALENSEPISALTNRFIAAMHADSDELGLMHPDQEPRATDYIQQMQGMIGKLIENELAYQGEDGDVNFAVRLLPRYGQLSGKTLDELNAGERVAIGGGKRDPLDFVLWKSAKPEEPADTRWNSPWGEGRPGWHIECSAMSCDLLGAHFDIHGGGADLQFPHHENEIAQSEGALYGQNRQENDAPFVNYWMHNGHIRVNEEKMSKSLGNFFLIRDVLKSFDPEVVRFFMLKAHYRSPINYSDAQLEEARSGLTRLYTALTHIPEVDTVPIDLQNPWAKRFADAMNDDFNTPEAIAVLFDLASEVNRAQGAEKQMLAGLLKSLGGTLNFLQRDPTNFLQAGSKDQSGLSSEQIEEHIAARVAAKQAKDFAKADGIRKALLEQGVVLEDKPGGITEWRRA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit ATP binding. The designed protein sequence is ",
    "response#1": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYV",
    "response#2": "KEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYGAPYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRSATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPLQDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMFLSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFENSSFRGLYQFIRFIDELIERGKDFGEENC",
    "response#3": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR"
  },
  {
    "reference": "MDVILLERVAKLGHMGDIVRVKNGYARNFLLPGGKALRATEANKKHFESQRAHLETRNQELRDQAAAIGEKLDGQTFVIIRQAGETGQLYGSVSPRDIAEAAIAAGFDVHRNQIALTTPIKTIGLHEVPVVLHPEVSVNVTVNIARSPVEAERQAKGEEINVVEETNLDELGLEIGAALADAGGSLGDR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Binds to the 23S rRNA..2. The protein design must be focused on enhancing the efficiency of translation. The designed protein sequence is ",
    "response#1": "ANDLKSKLDLVKLEFFMKSNDSGKLFHSINSLNIADELLKLGFDIERKKIDIHHGTLKTFGTYDVTIKLYEGISSIIKVEIS",
    "response#2": "MPAKLKIEIERLVLWFKLKGNKESVHGAITAKKIKKELEIKGIFVDKQAIQTSGISTFGTSFVDIKLSSQTIAKLKINIS",
    "response#3": "AKLKIEIERLVLWFKLKGNKESVHGAITAKKIKKELEIKGIFVDKQAIQTSGISTFGTSFVDIKLSSQTIAKLKINIK"
  },
  {
    "reference": "MKNKRILQIGLIAAVVLISLFVFTLLTDDTRSFQQVDTSVAVEQLEDGNAAVVQIDDREQRVRIELREPITVEEQEGVEEIMAEYPARTAPQIFDNVQSSGADSYSTEVTEDSFLMGMLSFLLPMLLLFGLLFFFLTRMQSGGGMFGIGGSKHKELTKDMPKNTFADVAGADEAVDELQEIKDFLQDPTRYHALGAKIPRGVLLYGPPGTGKTLLARAVAGEADVPFYSISGSDFVEMFVGVGASRVRDLFKQAKENSPCIIFVDEIDAVGRQRGSGTGGGHDEREQTLNQLLVEMDGFGDREGVILMAATNRPDILDPALLRPGRFDRQIPVTAPDLSGREHILNVHAKGKPLGPDADLNSLAKRTAGMSGADLANVLNEAALLTARIGGTVITADALEEATDRVIGGPRRSSQIISEKEKKVTAYHEGGHTLAAWALKDIERVYKVTILARGRTGGHAMTASEDDKGMYNRDELFARLVFAMGGRAAEELVFGEPTTGASSDIEQATKIARSMLTEYGMSPSLGVVKYGQEQGDPFSGRGGGGTLEVSPAVAAKIDEETHRLLEDAHQQAYEILRDHRDYLDRLAEKLLEKETLRRPDLEVLFDGIEPREAHDVFPGENDRFSRQFGREPVKTPVELALERGEEPPKRYSLLQASQDARAKREAELAAAKASEEGSSASGGQHRQDEKEPAVSTALGSGPRYGGTPPPADWKVPGWPPNNNPYASTPAQAKEEHPGMKNYGLAEPQRAEDPPTEVIGFRLPDHEQP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have ATP hydrolysis activity and be active in plasma membrane.2. The active site active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "FSKELGDGGFGTVYYGTLKDGRAVAVKRLFERSLKRVEQFKNEIDILKSLKHPNLVILYGCTTRHSRELLLVYEYISNGTLAEHLHGNQAQSRPICWPARLQIAIETASALSYLHASGIIHRDVKTTNILLDSNYQVKVADFGLSRLFPMDQTHISTAPQGTPGYVDPEYYQCYRLNEKSDVYSFGVVLSELISSKEAVDITRHRHDINLANMAISKIQNDAVHELADLSLGFARDPSVKKMMSSVAELAFRCLQQERDVRPSMDEIVEVLSS",
    "response#2": "PKLGRELGRGQYGVVYLCDNWAGRHPCALKSVVPPDDKHWNDLALEFHYTRSLPKHERLVNLHGSVIDHSYGGGSSIAVLLIMERLHRDLYTGLKAGLVLKERLQIALDVVEGIRFLHGQGLLHRDIKLKNVLLDKQNRAKC",
    "response#3": "FSKELGDGGFGTVYYGTLKDGRAVAVKRLFERSLKRVEQFKNEIDILKSLKHPNLVILYGCTTRHSRELLLVYEYISNGTLAEHLHGNQAQSRPICWPARLQIAIETASALSYLHASGIIHRDVKTTNILLDSNYQVKVADFGLSRLFPMDQTHISTAPQGTPGYVDPEYYQCYRLNEKSDVYSFGVVLSELISSKEAVDITRHRHDINLANMAISKIQNDAVHELADLSLGFARDPSVKKMMSSVAELAFRCLQQERDVRPSMDEIVEVL"
  },
  {
    "reference": "MDLLKSPLYSKYIESNAKLVNFAGWEMPISFSGLINEHESVRTSAGFFDISHMGVISLRGINPKEYIQKFFPTNLYSFSEGQGLYTLMLNEKGGIIDDLIIYDLGQQEEDISEIFLIVNASRYQTDFSWIKNNLNTNNISISNAKKDKVLFALQGKNSFKLFEEWIKSSISHIPYFGCEYKIFKHISSKEKIFFSKTGYTGENGLEILLSAKAAINLWDFLVSKNIKPCGLGARDTLRLEAGMHLYGQDLNETTTPYEAGLGWLVHLENNHDFFGREFLEKQSRFGINKKLVGLNIEGRAIGRKGCEVFKDGENIGTITSGSWSPTKQKAIAFAYIQNSYATLNNVVEILIRGKTFKGTITKRAFYKKDI",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should have a low Km value for (6S)-5,6,7,8-tetrahydrofolate + (R)-N(6)-(S(8)-   aminomethyldihydrolipoyl)-L-lysyl-[protein] = (6R)-5,10-methylene-   5,6,7,8-tetrahydrofolate + (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] +   NH4(+). The designed protein sequence is ",
    "response#1": "MGILNVTPDSFSDGGTHNSLIDAVKHANLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEVIRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAEVNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGMSRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVLDKELAHINWNDDAYDIHNLVRGLNPWPIAYTEYKGERMKLYKTKVLDKEVSNKPGTIIEVNKDGVKVVCGKNVLIIEKVQFPNGKPLTIEQYINGTR",
    "response#2": "MGILNVTPDSFSDGGTHNSLIDAVKHANLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEVIRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAEVNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGMSRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVRADEQVDWSAPAEAVHNRVRGMNPWPGAFTMDEGKILKILRGRLRHEGLPLPDPTGSAAHPGEILQIVGDEVAVATGAGVYWLSEVRPAGGKTMTAGAYARGD",
    "response#3": "MGILNVTPDSFSDGGTHNSLIDAVKHANLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEVIRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAEVNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGMSRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVLDKELAHINWNDDAYDIHNLVRGLNPWPIAYTEYKGERMKLYKTKVLDKEVSNKPGTIIEVNKDGVKVVCGKNVLIIEKVQFPNGKPLTIEQYINGY"
  },
  {
    "reference": "MSIRILPQEEIQQAASSFHNPPLLYANPKNLYARRAKRLRQLAEHNPFGDYLEFVANIVEVQLDLLENQPIANRVGELTAYLEAHQGVKPLDVKTFKRSDEWQKLLLAFIDKFKPYASDTVLATLEWLEKASNSELEMLADHLLNECYEEVGADKAVFLWAVLSLYWVQLTQQLPRNTKAEYGEERHTCPVCNSAPIASVVHFGETQGLRYLHCSLCESEWNMVRAKCSNCEQTGKLDYWSLDSMDAAVKAESCGDCESYLKVMYQDKDPHVEPIADDLGTLFLDAEMEQKGLARSAINPFLFQVE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a specific function within the cytoplasm.2. For general function, the protein need meet that Necessary for formate dehydrogenase activity. The designed protein sequence is ",
    "response#1": "IMGSGGVFTAEDAYEKIRNGY",
    "response#2": "YSLRIIYFALLNQPRFLPMSPINENHPNLTNPIIRLAMGSIFAGFILTMNIPPTSMVPLTMPPISKLSALIVTITGLLIAMELNSATNKSLMMSYIHTHNFSNMLGYFTYLFHRMYPLANLYLGQHIATMLIDLNWYEKTGPKGQANLHSTISSYISSTQKGLIKTYFLSFIISIPMIMLIF",
    "response#3": "SRDLLLFFIMWEFELIPVYLLLSMWGGKKRLYSATKFILYTAGGSIFLLMGVLGMGLYGSNEPTLNFETSANQPYPVALEILFYFGFLIGYAVKLPIIPLHTWLPDTHGEAHYSTCMLLAGILLKMGAYGLVRINMELLPHAHSIFSPWLIIVGAIQIIYAASTSLGQRNLKKRIAYSSVSHMGFIIIGICSITDTGLNGAILQMISHGFIGAALFFLAGTSYDRIRLVYLDEMGGIAIPMPKIFTMFSSFSMASLALPGMSGFAAELVVFFGLITSQKYLLMY"
  },
  {
    "reference": "MGVGIVSDERVLANVYHMYKPPQGGIHPREAANHHAEYLPKVLQEALAKADIDIKEVDGIAFSQGPGLGPCLRTVATAARVLSLKLGVPIVGVNHCIAHLEIGRFTTHAEDPVMLYVSGGNTQIISYAGHRYRVFGETLDIGIGNMLDKLAREMGVPFPGGPLIEKMALKGEKYIPLPYSVKGMDVSFSGLYTAAVKKLGKERKEDIAYSVQETAFAMLVEVTERALTHLHKDEVLLAGGVARNKRLREMLKIMTEERGATLHIPPDDLCIDNGAMIAYLGLLMLKHGKAMKIEETSVIQRFRTDYVEIPWEVKKHRKIYHNAPGAEAIIKENEFFGREVIQKIRVKKGYRLPALDIMLRKERTKKEARILHEIKNLGILAPIIYDIDDFELTIEKINGVSVVSVLNELDENEVRRILRKIGEIAAKMHRQNYAHGDFTTGNMILKNGKIYLIDFSMSEKNATVEEMAVDLHMFEESFKAAHYPHISLLHEFYDSYRSLMGDETIAHVEEIKRRRRYV",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein have enhanced adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP +   H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA.2. A protein that can perform ATP binding, metalloendopeptidase activity, iron ion binding, N(6)-L-threonylcarbamoyladenine synthase activity, zinc ion binding in cytoplasm to support tRNA threonylcarbamoyladenosine modification, protein phosphorylation.3. The designed protein should have a binding site that is specific to the target substrate, Fe cation ligand.4. For general function, the protein need meet that Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37.; Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine5. The protein must bind to Fe(2+) in order to perform its enzymatic function.6. The designed protein must have at least one Protein kinase domain. The designed protein sequence is ",
    "response#1": "MLTLGLESSCDETACAIVNEDKQILANIIASQDIHASYGGVVPELASRAHLHIFPQVINKALQQANLLIEDMDLIAVTQTPGLIGSLSVGVHFGKGIAIGAKKSLIGVNHVEAHLYAAYMAAQNVQFPALGLVVSGAHTAAFFIENPTSYKLIGKTRDDAIGETFDKVGRFLGLPYPAGPLIEKLALEGSEDSYPFSPAKVPNYDFSFSGLKTAVLYAIKGNNSSPRSPAPEISLEKQRDIAASFQKAACTTIAQKLPTIIKEFSCRSILIGGGVAINEYFRSAIQTACNLPVYFPPAKLCSDNAAMIAGLGGENFQKNSSIPEIRICARYQSDH",
    "response#2": "MLTLGLESSCDETACAIVNEDKQILANIIASQDIHASYGGVVPELASRAHLHIFPQVINKALQQANLLIEDMDLIAVTQTPGLIGSLSVGVHFGKGIAIGAKKSLIGVNHVEAHLYAAYMAAQNVQFPALGLVVSGAHTAAFFIENPTSYKLIGKTRDDAIGETFDKVGRFLGLPYPAGPLIEKLALEGSEDSYPFSPAKVPNYDFSFSGLKTAVLYAIKGNNSSPRSPAPEISLEKQRDIAASFQKAACTTIAQKLPTIIKEFSCRSILIGGGVAINEYFRSAIQTACNLPVYFPPAKLCSDNAAMIAGLGGENFQKNSSIPEIRICARYQKRNLILAFESSCDETSVSVIEDGHRVLSNIVATQIASHQRFGGVVPEVASRHHIEQITKCTKEALEQAGVSYQDLTAVAVTYGPGLVGSLLIGVTAAKTIAWAHQLPLVPVNHMAGHLYAARFVSDFTYPMLGLLVSGGHTELVYMKEEHDYQIIGETRDDAAGEAYDKVGRVMGINYPAGKTVDQWAAKGHDTFHFPRAMEKEDNFDFSFSGLKSAFINTVHNADQRGEVLDKYDLAASFQQSVVDVLVAKTIRALDEFPVKQLILAGGVAANQGLRKQLSAGLQAKHPEVQLLQAPLKYCGDNAAMIGAAGYVNYLHGDRELPPELVAKGAEANIYPDHYLGEEVLLKKRIPKGYRIGDIDTYLRKKRTKKEAKLLGEAKRCGVVTPLVYDVDQTESTITMGKIAGKEVKEVFSKKIPLNTSEIISISEGIGENVARLHDCGIIHGDLTTSNIILKSDVENQDHQLVFIDFGLGKISDLVEDKGVDLLVFKKAISGIHHEISQVCFDSIIKGYECARDCKEVVAKINEIESRGRYTSS",
    "response#3": "MLTLGLESSCDETACAIVNEDKQILANIIASQDIHASYGGVVPELASRAHLHIFPQVINKALQQANLLIEDMDLIAVTQTPGLIGSLSVGVHFGKGIAIGAKKSLIGVNHVEAHLYAAYMAAQNVQFPALGLVVSGAHTAAFFIENPTSYKLIGKTRDDAIGETFDKVGRFLGLPYPAGPLIEKLALEGSEDSYPFSPAKVPNYDFSFSGLKTAVLYAIKGNNSSPRSPAPEISLEKQRDIAASFQKAACTTIAQKLPTIIKEFSCRSILIGGGVAINEYFRSAIQTACNLPVYFPPAKLCSDNAAMIAGLGGENFQKNSSIPEIRICARYQLISNVVASSMDEHARYGGVIPEIASRAHAEAFVPCVSKALADANMTLSDVDAIAVSAGPGLAGCLAVGVSGAKSLAWAANKPIYGINHVIGHIAVTQLQFGPFPKDTLALIVSGGHTSLLHVEDVARHIDVVGTTLDDAAGECFDKVARLLGFPYPGGPHIDRHAQLGNPHAIKVPQGLTQGKAGQQHPYDFSFSGVKTAVARWIEQQQAEGNEIPIDDVCASLADSVATVLARKAMRGCEQYGSKTLIVGGGFSANSQLRAKLLEVGAKHGVEVRIPQIKLCTDNGAMVITG"
  },
  {
    "reference": "MASNLISRLLPSNTQGRSIYDSLRAHDEASGSDMDVEERAAMAIDEENLRFKDDELGNPADVISGESVTESTAFLSDQQQTPQKQGYNRKGKGKGRSHNLAESPRLLEEDSDDDVPASLLIEGNNRHADPETPNHIRARQTALPKRAPAVPGPSAQQIKEERDRAHWEMTQEQQKLHEDGGNRATGVPHTPQSIPQNTGFYLTGSPKDKAMYRWVNVTNLDNFIGDLYAYFEGAGIWCIVLAKVIDILTLVFVAVFTTFLTQCVDYKKIYRGDARSLEYALVPQCTKKISGMSNVAIWLVCLFVLYRVYQLLTDLPRLMKMRDFFTYLLEIQDSNMQTVSFQDVIARLMALRDANPMTVERISPDNRKFVLGTQSKQRLDAHDIANRLMRKENYLIALFNKEILDLTLPLPFLQGRQLFSKTLQWNLEWCILDFVFNDYGQVRQLVLKDSHRRELSDGLRNRFLFAGLMNVICAPVIVIYVVIVYFFKYFNQYHKNPAALGSRGYTPLAEWKFREFNELHHLFNKRLNMSYPFASRYLNQFPNVKTAHMAKFITFMAGAVVSVLVVATVWDSEVLAGFDITSERPVLFYIGVFGSLWAITNGMIPEENEVFDPEYALRQVIEYTHYMPNHWQDRLHSDEVKREFSTLYQLKLMIFIEEVFSIIITPFLLWFSLPKCADQIIDFFREFTVHVDGVGYVCSFAVFDFKKGDGRAPTQGKAKSDMREDYYSTKHGKMAASYYNFLDNYLLNPKTGVPGHNPPGLRQQFYPP",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.2. The designed protein should have a specific function within the phagophore assembly site, cytoplasmic vesicle membrane, phagophore assembly site membrane.3. The protein must exhibit the following characteristics: Required for mitophagy; Different machineries are required for anterograde trafficking to the PAS during either the Cvt pathway or bulk autophagy and for retrograde trafficking..4. We need a protein that can perform a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-   phosphate)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-   3-phosphate)(out) with high efficiency. The designed protein sequence is ",
    "response#1": "MQITKAGNSNEGMINTSKTYIVYSIQLINNDDPSDEIQTRRRYSDFESLRDVLKKIFPLIIIPPIPPKNYFKFSMLNDLVSGNISQSSNTSSNNSTGANTSTNTTNGLGSNSTNYSYINSTHLNKNKLIEHRKRLLSNFLNNCLAIPQIRNLEFFAKFLDPNANWPYWFEIIVSDPQKRTGDPGSSSGYVSYQISTKTNNTSFYDNRGDPESIIVVHRRYSDLLLLHDILLNRFPTCIIPPLPDKKVFQYIAGDRFSQRFTQKRCHSLQNFLRRVSLHPDLSQSKVFKTFLVSSYRDRVVRRRYSEFESLRKILIKLFPMTLIPPIPEKQSLTSYGKSIAGSNANYVLPSEAAGCDLAVSVINGSVNLNDQKMIRHRIRMLTSFLNRLLQNEEVTKTSIIGDFLDP",
    "response#2": "MQITKAGNSNEGMINTSKTYIVYSIQLINNDDPSDEIQTRRRYSDFESLRDVLKKIFPLIIIPPIPPKNYFKFSMLNDLVSGNISQSSNTSSNNSTGANTSTNTTNGLGSNSTNYSYINSTHLNKNKLIEHRKRLLSNFLNNCLAIPQIRNLEFFAKFLDPNANWYRL",
    "response#3": "LNCKNHNVLSNYNVANKDILLKNELDTPPSKTSEMWYLAPCEDGSRRQRPSECSSDDLMCAIRHVKVDGSDHLTQKLDVHKSAKYEVEEVESGGFDIRFLGVKWGSNTIDATLSYSCDTNLKTDELVTTIWNSEYVLIEMSGPSGCKP"
  },
  {
    "reference": "MLDSQTIATVKSTIPLLAATGPKLTAHFYDRMFEHNPELKEIFNMSNQRNGDQREALFNAICAYATNIENLAALLPAVERIAQKHASFNIKPEQYQIVGHHLLKTLEEMFNPGDEVIEAWGKAYGVLADVFIQRESQIYQQSETTEGGWRELRPFKILKKEKQSDLITSFILVPEDGSRVADFQPGQYLAVYIRHPSLPHQEIRQYSLTNEPNGEYYRIAVKREGQGQVSNFMHDIAQEGDVIQIAPPHGDFFLEVKENTPVALISGGVGQTPMLGMLRTLQARGHQGEIQWLHAAENSKVRAFADEVNTIISAMPQAQSHVWLQETSADDRIDIDFNYQGLMDITPLADKLNNPEMHYYFCGPVGFMQHVAKQLQALGVDADRMHYECFGPHKVV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must be engineered to have a high affinity for heme b in order to increase its catalytic activity.2. The protein must exhibit the following characteristics: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds.3. The protein should be able to modulate response to nitrosative stress in a way that leads to a desirable outcome.4. The protein should be able to bind NADP(+), FAD ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MLDQKTIDIVKSTAPVLDQHGETLTRHFYQRMFTHNPEVVPFFNPAHQKSGSQQRALAAAICAYAANIENLEVLGNAVELIAQKHASLHIKPEHYPIVGENLLASIREVLGDAATDDIINAWANAYGFLANILINVE",
    "response#2": "MLSQQTIAIIKSTVQVLEVHGTEITTTFYQSLFNAHPELLNLFNHANQKQGKQQAALANAVYAAALHIDRLEAILPVVKQIAHKHRSLGILPEHYPIVGQHLLGAIKTVLGDAATDDMIGAWGEAYGVIADAFIG",
    "response#3": "MLDQATIAVIKSTIPLLESAGPALTQHFYQRLFSHHPELKDVFNLAHQRSGGQPLALFSAVAAYARNIDNLGALAGAVERIAHKHTGFLIQPEQYHIVGSNLLATLKEMGGNAVTEEVLAAWGKAYGVLADIFE"
  },
  {
    "reference": "MPTRFIFVAGGVMSGIGKGVAVASIGRILKSKGFGVTAVKIDPYINVDAGTMNPIEHGEIFVTEDGDECDQDVGNYERFLDENLSRANYITTGRVYQAVINRERNLEYGGRCVEVVPDIPNEVISRIKKAAKKTQADFVLIEIGGTVGEYQNLLFLEAARMIHLREPKNVLFILVSYLPIPEKIGEMKTKPTQTAVRLLNEAGIQPDIILGRSKVPLDAPRKRKISIFCNVQKEDVISAPDIESIYEIPINFEKENLGNRILKKFDLRAKKPSTRAKHGAGPVPYRNEVSGTGQNLKDWEALIKTIKNTKKPVKIGIVGKYFETGSFTLMDSYISVIEAIKHASWHYKRKPEIRWLSSEGYEKNPQSVKELKNYDGIIVPGGFGSRGIEGKIKAIEFCRKNKIPYLGLCLGMQLAVVEFARNVCGLKGANSTEFNPKTKYPVIDVMPEQKALIREKKYGGTMRLGGYKCELKKGSRSWKSYGMLKCVSERHRHRYELNNEFREVLEAKGMIMAGINPERDLVEIIELKNHPFFVSSQFHPEFKSRPLRPHPLFREFIKSSLWLRN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.2. A protein with glutaminase activity, metal ion binding, CTP synthase activity, ATP binding functions is required to enable glutamine metabolic process, 'de novo' CTP biosynthetic process.3. The designed protein should contain one or more CTP synthase N-terminal domains that are essential for its function.4. A protein that is capable of catalyzing reactions within the CTP biosynthesis via de novo pathway, CTP from UDP: step 2/2 pathway with high efficiency and specificity.5. A key design requirement for the protein is the optimization of the Nucleophile; for glutamine hydrolysis, active site, Nucleophile to achieve high catalytic efficiency for H2O + L-glutamine = L-glutamate + NH4(+).6. The protein should be able to bind Mg(2+), ATP, CTP, L-glutamine, UTP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "IFVTGGVASSLGKGLTASSLGHLLRARGLRVVMQKLDPYINVDPGTMNPFQHGEVFVTKDGTETDLDIGHYERFLDIELSGAANSTTGQVYSRVLAKERAGEYLGECVQVIPHITDEIISRMRAQAEPDEDGQVPDVIITEIGGTVGDIESQPFLEAARQVRQLIGRDNCFFIHVSLVPYLAAGGELKTKPTQHSVAALRSIGIQPDAIVLRCDRELPESVRQKVALMCDVDREGVIECQDAASIYEIPVTLHNEGLDAYVVRRLGLPFRDVDWSAWDALLERGHAPAPEVRIGLVGKYVELPDAYISIVESLKHAGFCYNTEVDIKWIYAEKVEKESAEKFLHDVDGILVPGGFGERGIEGKIATAKYARENKVPYLGICLGMQCAVIEFARHVCGLMGANSSEFDPHSPHPVVHLMPDQENIEQKGGTMRLGAYPCHIKEGTKTYDVYKTGIISERHRHRYEFNNDYRKIMEEKGMIIAGLSPDGRLVEIIELKDHPWFVACQFHPEYKSRPNRPHPLFLGLFVAAAGRLAAEAGEALPFHYGGSR",
    "response#2": "KYIFVTGGVVSGLGKGIAAASLGLILKSRGYKVVNQKFDPYLNIDPGTMNPYQHGEVFVTDDGAETDLDLGHYERFTDASLSQSSNTTAGRVYLAVLNKEREGGFHGGTVQVIPNITEEILRRMLLSTEETKADVIITEIGGTVGDIESLPFMEAIRQMKYKVGEDNCCYIHLTLVPYMKKADELKTKPTQHSVKELQELGIQPDIIMLRTEQPVDTGTREKLASFCNVDTSHVIQNLNARSIYEVPLQMEQEGLGKAVCETLGLHVGKPEPDLSDWQAMVARVKAPAGKVRIGLVGKYVELPDAYISIVESLKHAGFCYNTEVDIKWIYAEKVEKESAEKFLHDVDGILVPGGFGERGIEGKIATAKYARENKVPYLGICLGMQCAVIEFARHVCGLMGANSSEFDPHSPHPVVHLMPDQENIEQKGGTMRLGAYPCHIKEGTKTYDVYKTGIISERHRHRYEFNNDYRKIMEEKGMIIAGLSPDGRLVEIIELKDHPWFVACQFHPEYKSRPNRPHPLFLGLFLAACDRRVAAG",
    "response#3": "RFIFITGGVVSSLGKGLMAASLAALLQARGYRVRIRKFDPYLNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTGVAARQSDNVTSGRIYQQIIQRERRGDYLGATVQVIPHVTDAIKAFALADTDDLDFVLCEIGGTVGDIESLPFIEAIRQIRNDLGRGNSVSVHVTLVPYIAAAGELKTKPTQHSVRELAALGVQPDVLVCRCEQPLPASERAKIALFCNVPANAVIPALDAKSIYAVPVQYHEEGLDEAVRSRFGLEHPPAPDLSRWREIVDRIRNPEGEVKVALVGKYVQLNDAYLSVVEALRHACIDRDASLDLHWISAEQIETQGAESLLRGMDAVVVPGGFGHRGVDGKVAAIRWAREERVPFLGLCLGMQCAVIEWARNLAGLAGATSAELDPDSPHPVINLLPEQQDVIDLGGTMRLGVYPCRLTAGTMAASLYGEEVVYERHRHRYEFNNAYRNLFQAGGYTISGTSPDGRLVELIEREDHPFFAACQYHPEFLSRPGKPHPLFRGLVGAALGRS"
  },
  {
    "reference": "MKTDKIRAMFLQFFKEKNHKIFKSSSLIPKDDNSLLFTNAGMNQFKKIYLKNKKKNQLSIATSQYCIRTGGKHDDLKKVGHTPYHHTLFEMLGNFSFGEYFKENAIKYAWEFLTHKKWLNLSKKKIWITYYYNDNETKKIWLNIIKIKNTHLIKIYDKKNIKYNSDNFWKMGDLGPCGPSTEIFYYQKKNRKEKKSIKSYKELEKYCLEIWNLVFMEFNQITKKKIIKLPIPSIDTGMGLERISVILQNVKSIFHTNNFIQLIEAIKKKIKIKIKKKNNVSLKIISDHIRTSTCLIAENILPGNEGRNYVLRKIIRRALCHGYFIGLQKPFFYKLSELIIYNMKKMNYDFHIQEKIHKIKKILFYEEKQFYYILENGLQKLKKIIKNIKKNKICEQKIFTLYDTYGLPIQITQKFCKKKNISFNINKLNLIIKNNKKKQKNKEKKKKNFSFITFKKKSIFDGYHIYEKKSKIIQIIYKNKNVLKLSKSQLGIIILDITPFFSKSSGQIGDSGKILNDKGIFIVQKTKKFDNYILHIGYIKHGCINIKDIVHAKINKKKRKTIQSNHSATHLLNAALCKIFSEKIIQKGSFINDKYLRFDFFCNKTLTEEKINYLENIINKKIQKNIPINFVITSFKKAKKKKIKFLLEKKYKKTVRIIYIKNFSIELCNGTHHDNTGKIGCFKIIAYNNIGNEIKRITAITGMEVIQYFNDKDKINKKLENLFSTNIHNIYLIAKNIVNKNKKLLEENIKLKNKNIIEISKILISKAY",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The ideal protein would be one that plays a critical role in alanyl-tRNA aminoacylation through ATP binding, alanine-tRNA ligase activity, tRNA binding, zinc ion binding within cytoplasm.2. The protein should have a low Km value for ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-   tRNA(Ala).3. The protein must exhibit the following characteristics: Catalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala); Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain..4. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.5. The designed protein should have a binding site that is specific to the target Zn(2+) ligand. The designed protein sequence is ",
    "response#1": "MDIRNEFLQFFQNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPRPSIPRAASSQLCMRAGGKHNDLENVGYTARHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKPKDLYISVHEKDDEAVKLWEKFVPVDRIKKMGDKDNFWQMGDSGPCGPCSEIYIDQGEKHFKGSEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFDSSLFAPLMEEISELTSLDYASEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRILRRALRHGYLMGLKEAFLYKVVGVVCEQFANTHAYLKESKEMVVKECFEEEEHFLETLESGMELFNLSLKHLNENKIFDGKIAFKLYDTFGFPLDLTNDMLRSHGACADMQGFELCMQEQVKRSKASWKGKQNNADFSAILNAYAPNVFVGYETTECSAKVLGFFDSDFKEITDANPNQEVWVLLEKTPFYAEGGGAIGDRGALFKDNGEVAIVLDTKNFFGLNFSLLEIKKALKKGDQVIAQVSDERFEIAKHHSATHLLQSALREVLGSHVSQAGSLVESKRLRFDFSHAKALNDEELEKVEDLVNAQIFKHLNSQVEHMPLNQAKDKGALALFSEKYAENVRVVSFKEASIELCGGIHVENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKSQDEKLKQIAAIVKAPAKEVPARVEALLAQLKDTQKELARLKAQLASGATTDLVGDAVVV",
    "response#2": "MTGNELRARFLKFFEDRGHTVVPSSLLIPHNDPTLLFANAGMNQFKDCFLGMEDRGYTRATSSQKCVRAGGKHNDLENVGRTARHHTFFEMLGNFSFGDYFKKEAIAFAWEFLTKDLGLDKDRLYVTVYTDDDEAADIWHHQEGVPRERIFRFGEKDNFWSMGDTGPCGPCSEIFWDNGPEVGCGSPDCTVGCDCDRYMEIWNNVFMQFNRSADGTMTPLPKPSVDTGMGLERICTVMQGVKSNYDTDLLQGVIRHVERLSGKRYRENEKDDVSMRVIADHARATTFLICDGVLPSNEGRGYVLRRIMRRAARHAKMLGFAEPVLYRTVDAVNEMMGGSYPELLEREEYIKKVIRAEEERFAETLDRGLAILNDAVAQLKGEGKTVIPGETLFRLYDTFGFPTDLTADIVRAEGFTIDEPGFEACMGRQREQAREHWKGSGEEGIAAVHKELHNRGVRSVFVGYDEKCSYAAIGSILRGGSEVAEAKAGEDVEIITDRTPFYGESGGQAGDTGTISTGSAHVRVTGTIRPYPDLIVHRGTVVEGTIKTGEACDLKVASVDRDATARNHTATHLLQTALRRVLGEHVKQAGSLVAPDRLRFDFTHFAAMTPEEIRRVEEIVNTFIMENDTVHAREMAVEEAMESGATALFGEKYGDRVRVVKVGEVSAELCGGTHVRAAGDIGSFKILSEAGIAAGVRRIEALTGMGALRHIQEQDERVKQAAALLKAQPSEVIERITSLQERQKTLERELEKLKGKLASGAEQDLVAQAVE",
    "response#3": "MDIRNEFLQFFQNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPRPSIPRAASSQLCMRAGGKHNDLENVGYTARHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKPKDLYISVHEKDDEAVKLWEKFVPVDRIKKMGDKDNFWQMGDSGPCGPCSEIYIDQGEKHFKGSEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFDSSLFAPLMEEISELTSLDYASEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRILRRALRHGYLMGLKEAFLYKVVGVVCEQFANTHAYLKESKEMVVKECFEEEEHFLETLESGMELFNLSLKHLNENKIFDGKIAFKLYDTFGFPLDLTNDMLRSHGACADMQGFELCMQEQVKRSKASWKGKQNNADFSAILNAYAPNVFVGYETTECSAKVLGFFDSDFKEITDANPNQEVWVLLEKTPFYAEGGGAIGDRGALFKDNGEVAIVLDTKNFFGLNFSLLEIKKALKKGDQVIAQVSDERFEIAKHHSATHLLQSALREVLGSHVSQAGSLVESKRLRFDFSHAKALNDEELEKVEDLVNAQIFKHLNSQVEHMPLNQAKDKGALALFSEKYAENVRVVSFKEASIELCGGIHVENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKQQNDLLSEISTTLKAKPSELTQRINQLLTQTKELQKENESLKAKIASQSSANLFEQAVDV"
  },
  {
    "reference": "MEVNNMSKTRVYELAKELNISSKDLLSKLSDLDIKVKNHMSTLEDEEVELIKDLLAEKPKEEKQKDQKNHEQEAQDKEEKEIEEDSFYEDREEKRAYKKSFKKGGKKNKKLQKKFVSEESAKEDEIKIITIPEFLTVKELAEKMKVNPTEIIKKLIAQGIMVTVNQQIDFETASKIAEEYGFLVDKEEVKDELEAIFEDTPDREEDLKPRPPIVTVMGHVDHGKTSLLDAIRKTNVTMKEMGGITQHIGASVVEINDKKVVFLDTPGHEAFTAMRARGASVTDIVVLVVAADDGVMPQTIEAINHVKAANVPLIVAINKIDLPTANPDRVKTELSELGLVPEEWGGNTICVPVSAKKNIGIDDLLEMILLVAEMEDLKANPNKPARGTVIEAKLEKGKGPVATVIVQNGTLQVGDAVIAGTTYGKVRAMFDDKGRKIKKAGPSMPVEILGFSEVPEAGDKFVVVENEKKARELAEKRREVQRELELKKKQKVSLEDLFRQIQEGTVKELNVIIKADVQGSVEALRKSLEELSNEEVRIRVIHGAVGAITETDVMLASASNAIIIGFNVRPETNAKALAEKEKVEIKLYRIIYDAIEDVKAAMKGMLEPKYKEVELGRAEVRAVFKIPGVGNVAGCYVLNGKIARNADVRIVRDGIVIYEGKIASLKRFKDDVREVQQGFECGIGIEKFNDIKEGDIIEAYTMEEIPR",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits2. The protein must have high specificity towards GTPase activity.3. The designed protein should have a high specificity for GTP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "PVVTIMGHVDHGKTSLLDYIRKTRVAAKEAGGITQHVGAFEAKTSKGKIVFIDTPGHEAFTTIRARGANVADIAIIVIAADDSLMPQTREAIAHAQAAKVPMIVAINKVDLPQADPERVKTDLTQLNLVPEEYGGDLIVVPVSAKTGEGVEDLLEYIPRPPVITIMGHVDHGKTSLLDAIRDAKVVAGEAGGITQHIGAYQVTTDSGAVLSFLDTPGHAAFTSMRSRGAQVTDIVVLVVAADDSVMPQTVEAINHAKAAGVPMIVAINKIDKPAADPNKVRAELLQHEVIVEAMSGEVQDVEVSAHTGQGLDELLEAIALQAEILPRPPVITIMGHVDHGKTSLLDAIRDAKVVAGEAGGITQHIGAYQVTTDSGAVLSFLDTPGHAAFTSMRSRGAQVTDIVVLVVAADDSVMPQTVEAINHAKAAGVPMIVAINKIDKPAADPNKVRAELLQHEVIVEAMSGEVQDVEVSAHTGQGLDELLEAIALQAEILPRPPVITIMGHVDHGKTSLLDAIRDAKVVAGEAGGITQHIGAYQVTTDSGAVLSFLDTPGHAAFTSMRSRGAQVTDIVVLVVAADDSVMPQTVEAINHAKAAGVPMIVAINKIDKPAADPNKVRAELLQHEVIVEAMSGEVQDVEVSAHTGQGLDELLEAIALQAEILEKSPAEKPIRLTVAGRPNVGKSTLINTWLGEERLVAFDMPGTTRDAISVPFERAGQKFELIDTAGLRRKGRVFEAIEKFSVVKTLQAIESANVVLLLIDATQGVTDQDAHIAGYVLESGRAVVLAVNKWDAVDDYQRQMLERSIETRLSFLKFAPMHFISAEKRQGEFTARVFIIWHPTAITVGYTPVIHIHTASVASRIVEIKAKLDPRTGKVVEENPQFIKMGDAAIVRFKPIKPLVVEKYSDFPPLGRFAMRDMGKTIGIGV",
    "response#2": "PVVTVMGHVDHGKTSLLDYIRRAKVAAGEAGGITQHIGAYHVETDRGMVTFLDTPGHAAFTQMRARGAKATDIVILVVAADDGVMPQTREAVQHAKAAGVPLVVAVNKIDKPGADLDRIRNELAVEGVTSEDWGGDTPFVKVSAKMGTGVDELLEAIAGKPHLNLVVIGHVDHGKSTLVGHILYRLGLVDQKTIQMLEEEAKKRGKESFKFAWLLDKLKEERERGVTIALTYMKFETRRYIFTIIDAPGHRDFVKNMITGASQADAALLVVSARKGEFEAGMSPEGQTREHAILAKTMGINQLIVAVNKMDATEPPWSQKRYEQIKTILGKFLKSLGYDISKVPFIPVSAWTGDNLIERSPNMPWYNGPTLVEALDSLEPVITIMGHVDHGKTSLLDAIRDAKVVAGEAGGITQHIGAYQVTTDSGAVLSFLDTPGHAAFTSMRSRGAQVTDIVVLVVAADDSVMPQTVEAINHAKAAGVPMIVAINKIDKPAADPNKVRAELLQHEVIVEAMSGEVQDVEVSAHTGQGLDELLEPRPPVITIMGHVDHGKTSLLDAIRDAKVVAGEAGGITQHIGAYQVTTDSGAVLSFLDTPGHAAFTSMRSRGAQVTDIVVLVVAADDSVMPQTVEAINHAKAAGVPMIVAINKIDKPAADPNKVRAELLQHEVIVEAMSGEVQDVEVSAHTGQGLDELLEAIALQAEILTRPPVVTIMGHVDHGKTSLLDAIRKAKVVAGEAGGITQHIGAYQVKTDSGQLLSFLDTPGHAAFTSMRSRGAQVTDIVVLVVAADDAVMPQTIEAINHAKAAEVPMIVAINKIDRPAADPTKVRTDLLQHEVIVEQMSGDVQDVEVSAITGQGLDDLLEAIALQAEILTRPPVVTIMGHVDHGKTSLLDAIRKAKVVAGEAGGITQHIGAYQVKTDSGQLLSFLDTPGHAAFTSMRSRGAQVTDIVVLVVAADDAVMPQTIEAINHAKAAEVPMIVAINKIDRPAADPTKVRTDLLQHEVIVEQMSGDVQDVEVSAITGQGLDDLLEAIALQAEIL",
    "response#3": "VVTVMGHVDHGKTSLLDYIRRAKVAAGEAGGITQHIGAYHVETDRGMVTFLDTPGHAAFTQMRARGAKATDIVILVVAADDGVMPQTREAVQHAKAAGVPLVVAVNKIDKPGADLDRIRNELAVEGVTSEDWGGDTPFVKVSAKMGTGVDELLEAVSRAPVVTVMGHVDHGKTSLLDYIRRAKVAAGEAGGITQHIGAYHVETDRGMVTFLDTPGHAAFTQMRARGAKATDIVILVVAADDGVMPQTREAVQHAKAAGVPLVVAVNKIDKPGADLDRIRNELAVEGVTSEDWGGDTPFVKVSAKMGTGVDELLEAVLLQAEILPRPPVITIMGHVDHGKTSLLDAIRDAKVVAGEAGGITQHIGAYQVTTDSGAVLSFLDTPGHAAFTSMRSRGAQVTDIVVLVVAADDSVMPQTVEAINHAKAAGVPMIVAINKIDKPAADPNKVRAELLQHEVIVEAMSGEVQDVEVSAHTGQGLDELLEAIALQAEILSRAPVVTVMGHVDHGKTSLLDYIRRAKVAAGEAGGITQHIGAYHVETDRGMVTFLDTPGHAAFTQMRARGAKATDIVILVVAADDGVMPQTREAVQHAKAAGVPLVVAVNKIDKPGADLDRIRNELAVEGVTSEDWGGDTPFVKVSAKMGTGVDELLEAVLLQAEILSRAPVVTVMGHVDHGKTSLLDYIRRAKVAAGEAGGITQHIGAYHVETDRGMVTFLDTPGHAAFTQMRARGAKATDIVILVVAADDGVMPQTREAVQHAKAAGVPLVVAVNKIDKPGADLDRIRNELAVEGVTSEDWGGDTPFVKVSAKMGTGVDELLEAVLLQAEILTRPPVVTIMGHVDHGKTSLLDAIRKAKVVAGEAGGITQHIGAYQVKTDSGQLLSFLDTPGHAAFTSMRSRGAQVTDIVVLVVAADDAVMPQTIEAINHAKAAEVPMIVAINKIDRPAADPTKVRTDLLQHEVIVEQMSGDVQDVEVSAITGQGLDDLLEAIALQAEIL"
  },
  {
    "reference": "MGLLSYILLGFTHPSELRAMIGYKVWRDPLNDIKANPQASGWDRQRMRDCWGFLDLTSRSFAAVIKELKGELSRVICLFYLVLRALDTVEDDMTIPAQRKIPLLVNFYKYLEQPGWNFTESGPNEKDRQLLVEFDKVIAEYQLLDVGYKTVISDITAKMGAGMASYIELSAKGPLKVAMWKHFDLYCHFVAGLVGEGLSRLFSESKLERPWLGHQLELSNHMGLFLQKTNIIRDYAEDCEEGRYFWPQQCWGDDFAKFESQPDVAKGIIEIKPGHFRPADNELGQRSMYVLSSMLLDAMSHATHALDYLALLKEQSVFNFCAIPQVMAIATLELMFNNPDVFKKNVKIRKGVAVGLILRAVNPRDVAYTFLHYSRKMHARLSPADPNFTRWSVELARIEQWCETYYPSFIAAASEGKPTDIRANALRSWSESRRTQALILKQAKLNGSDASTLDAKSVLEAAQNSALDPRDLMTEDERAAQDKRDRDQMVKFFLIILVGMVTFMGIVALITWEIVWWWTMDTPDPLSVYVKHAYYLVKTQGWSTVKEVLRTTRLSFEHVWKHGLTSPPKLEL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be designed to have high stability and activity towards 2 (2E,6E)-farnesyl diphosphate + H(+) + NADH = 2 diphosphate +   NAD(+) + squalene.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. A protein that can modulate the overall flux of metabolites through the lanosterol biosynthesis, Terpene metabolism pathway, enhancing or suppressing its activity as needed.4. The ideal protein would be one that plays a critical role in isoprenoid biosynthetic process, farnesyl diphosphate metabolic process through squalene synthase activity, farnesyl-diphosphate farnesyltransferase activity within endoplasmic reticulum membrane.5. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.6. For general function, the protein need meet that Required for the biosynthesis of ergosterol .; Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene The designed protein sequence is ",
    "response#1": "KACFRHLELTSRSFSAVIQELNPELLMPICLFYLVLRGLDTIEDDMTIDLAKKEPLLREFADLMEIDGWTFTENGPNEKDRELLVHFDDVIAELKKVKKPYYDIIREITVKMGNGMADYALNAEHNTNGVNTIEEYELYCHYVAGLVGEGLTRLFVESNLANPALLERMELTESMGQFLQKTNIIRDIHEDYVDKRRFWPKTIWSKYVNTWDDMFKPENREKALQCSSEMVLNALKHTEDCLFYMAGMRDQSVFNFVAIPQAMAIATLELVFRNPAIFERNVKITKGDAQSKHFPQAPVPAILVVSADRKDTPSTSGMQQTVHTSDLFKERVATVVPRRYGEMAAAIRARDFATFARLTMQDSNSFHATCLDSFPPIFYMNDTSRRIVKLCHLINEFYNETIVAYTFDAGPNAVLYYLAENEARLCGFLSAVFGANDGWETTFSTEQRATFAAQFDECVRGKLATDLDDELHRGVARLIFTKVGPGPGGEGVG",
    "response#2": "AILVVSADRKDTPSTSGMQQTVHTSDLFKERVATVVPRRYGEMAAAIRARDFATFARLTMQDSNSFHATCLDSFPPIFYMNDTSRRIVKLCHLINEFYNETIVAYTFDAGPNAVLYYLAENEARLCGFLSAVFGANDGWETTFSTEQRATFAAQFDECVRGKLATDLDDELHRGVARLIFTKVGPGPGA",
    "response#3": "AILVVSADRKDTPSTSGMQQTVHTSDLFKERVATVVPRRYGEMAAAIRARDFATFARLTMQDSNSFHATCLDSFPPIFYMNDTSRRIVKLCHLINEFYNETIVAYTFDAGPNAVLYYLAENEARLCGFLSAVFGANDGWETTFSTEQRATFAAQFDECVRGKLATDLDDELHRGVARLIFTKVGPGPGGGR"
  },
  {
    "reference": "MAEVQREFVMIKPDGVVRGLVGEVIRRLERKGLKIVALEMRQLDRETAEKLYEEHRDKPFFEDLIEYVTSGPVVVMIVEGRKAVKVVRNIIGATDPAEAAPGTVRGDFALDIGRNVVHASDSPESAEREIEIVFGKDLSTIDYERCDEEWLYES",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must have a Pros-phosphohistidine intermediate active site that is highly conserved among related enzymes.2. For general function, the protein need meet that Major role in the synthesis of nucleoside triphosphates other than ATP3. The designed protein should be optimized for ATP binding, metal ion binding activity in order to improve nucleoside diphosphate phosphorylation. The designed protein sequence is ",
    "response#1": "MEKTFAIIKPDAFAAGNAGKILARIYQEGFTVIGLKKLCMSQREAEGFYAVHNKKPFFAELTKFMSSGPCIVMVLEAEGCIGKWRDLMGATNPADAKPGSLRREFGTIVGENATHGSDAPETAAVELEYFFSGLELT",
    "response#2": "MEKTFAIIKPDAFAAGNAGKILARIYQEGFTVIGLKKLCMSQREAEGFYAVHNKKPFFAELTKFMSSGPCIVMVLEAEGCIGKWRDLMGATNPADAKPGSLRREFGTIVGENATHGSDAPETAAVELEYFFSGLELK",
    "response#3": "MEKTFAIIKPDAFAAGNAGKILARIYQEGFTVIGLKKLCMSQREAEGFYAVHNKKPFFAELTKFMSSGPCIVMVLEAEGCIGKWRDLMGATNPADAKPGSLRREFGTIVGENATHGSDAPETAAVELEYFFSGLELR"
  },
  {
    "reference": "MSTPVVKRILLGITGGIAAYKTPDLVRRLRERGVAVRVVMTQGAQAFITPLTLQAVSAERVATDLLDAEAEAAMGHIELARWADQVLIAPASADCLARLAHGFADDLLTTLCLATEAPIAVAPAMNRVMWQAPATRANCELLAERGVRFLGPGEGDQACGESGSGRMLEPLDLVAALLDGPTETATAGPLAGRTVLITSGPTREALDPVRYLTNHSSGKMGHALAEAARDAGARVVLVSGPSRLSAPAGVEAVAVESARDMHREVMARVGEMDLFIGAAAVADYRPEQSAGRKIKKSDSDTALRLVPNPDILREVAALGEGRPFTVGFAAETDDLARHARDKLDRKRLDMIAANWVGADRGFHADDNALEVYWPGDGHASLARADKPALARQLIQLIAEHYHARSRGTENP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.2. The designed protein have DNA/pantothenate metabolism flavoprotein C-terminal domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MNVLIGVTGSIAIYKTCELIRLFIKNGDNVKVVMTKSAAKFISPLTFETLTRNKVLIEENEDWSSCINHIDYAKWADIYIIAPATANTLNKALVGIADNLLLQVYLATTSPTLFAPSANTNMYLHPTTQKAIKKLNCIEANSGLLACGDEGIGKMAEPREIFLRALL",
    "response#2": "KKLLLGVTGGVAAYKVAELARLLTQKGVVVQTVMTKSACRFVGPATFQSLTGLPVFTELWEANVASNMAHINLSRNVEMILIAPASADFIAKLACGLADDLLSTLCLARNCPMMIAPAMNRQMWENPATQRNINRLQDDGVIVAGPADGEQACGEVGPGRMLETEWIYEAAFRHFGLQGKKVLVTAGPTQESLDPVRFLTNHSSGKMGYSIARAAFILGATVRLIHGPTSLKPVPYIINQSITSAQDLFECVKRHHTHYDYIIMSAAVADYTPVETSTEKIKKNDNELILKLKKNPDILDYIGHHQVEHQVICGFAMETQDLIENASKKLNKKQCDLIIANHLKTEGAGFQGDTNVVTIERRSAGGELVIID",
    "response#3": "KKILLGITAGIAAYKTASLVRLFIKLGAEVKVIMTPASKDFITPLTLSTLSKNPVHSTFYKKEAENEMWNNHVDLGLWADYMVIAPATANTLSKMANGTCDNLLLATYLSSKCPVYFAPAMDLDMYLHPSTKESLEKLQNFGNNLIPATSGELASGLVGEGRMAEPQDIVSFIETEIFKRKLPLQGKKVLVTAGPTQESLDPVRFLTNHSSGKMGYSIARAAFILGATVRLIHGPTSLKPVPYIINQSITSAQDLFECVKRHHTHYDYIIMSAAVADYTPVETSTEKIKKNDNELILKLKKNPDILDYIGHHQVEHQVICGFAMETQDLIENASKKLNKKQCDLIIANHLKTEGAGFQGDTNVVTISLLSKDKEKTIHFANKTKLASRILEII"
  },
  {
    "reference": "MSVQKEEYDIVEKAQLSVSAESLTSDSESISHNPFDDFHKAERWRKVYESSGYEGLSKFDPEFTWTKDEEKKLVRKMDLKIFLWVFIMFAFLDLIRKNIARAVSDNFIVDLKMNTNDYNLGQTVYLVIFLASELPGNLLSKRFGPERVIPVQIVLWSVICITQAGLKNRGQFIATRCLLGMVQGGFIPDNILYLSYYYTGAELTFRLSFFWCAIPLFQILGSLLASGIIEMRGIHNLAGWQYLFIIEGFLSLSVGVASFYLMRRGPTQTGESAFHKGKSLFTEYEEKIMVNRILRDDPSKGDMSNRQPVTFKEILYTLTEFDLWPLFIQGITAFISLQTVGSYLSLILKSLNYSTFLSNILAIPGQALLLINLPLAALLSRKLKEKSLCVGIANVWVLPFIVSLVALPTDTNPWIKYILLTGILGLPYTHSILAGWVSEISNSVRSRTVGTALYNMSAQVGAIIASNMYRNDDKPYYTRGNKILLGFTCFNICMAVATKFYYISRNKYKDRKWNSMTKEEQINYLDTTKDKGMKRLDYRFIH",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization.2. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.3. The designed protein should have sulfate transmembrane transporter activity, sulfur compound transmembrane transporter activity to facilitate sulfate transport, sulfur compound transport. The designed protein sequence is ",
    "response#1": "PDVTFTVLLAFLTAFDGQIVTVAKAAAGYGNTGLLTVIFLYWVAEGITQTGGLELIMNFVLGRSRSVHWALARSMFPVMCLSAFLNNTPCVTFMIPILISWGRRCGVPIKKLLIPLSYASVLGGTCTSIGTSTNLVIVGLQDARYTKAKQLDQAKFQIFDIAPYGVPYALWGFVFILLTQAFLLPGNSSRYAKDLLIAVRVLPSSSVAKKKLKDSGLLQQSGFSVSGIYRDGKYLSKPDPNWVLEPNDILYAAGEFDVVEFVGEEFGLGLVNADAETSAERPFTTGEESVFTPTGGAPYQKLVQATIAPTSDLIGRTVREVSWQGRFGLIPVAIQRGNGREDGRLNDVVLAAGDVLILDTTPFYDEEREDSKNNFAGKVRAVKDGAAKEFVVGVKVKKSSEVVNKTVSAAGLRGIPGLFVLSVDRADGSSVEASDYLYKIQPDDTIWIATDIGAVGFLAKFPGLELVQQEQVDKTGTSILYRHLVQAAVSHKGPIVGKTVRDVRFRTLYNAAVVAVHREGARVPLKVQDIVLQGGDVLLISCHTNWADEHRHDKSFVLLQPVPDSSPPKRSRMVIGVLLATGMVLTQIVGGLKSREYIHLWPAAVLTSALMLLTGCMNADQARKAIYWDVYLTIAAAFGVSAALEGTGVAASFANGIISIGKNLHSDGAALIAIYIATAMLSELLTNNAAGAIMYPIAAIAGDALKISPKETSVAIV",
    "response#2": "PDVTFTVLLAFLTAFDGQIVTVAKAAAGYGNTGLLTVIFLYWVAEGITQTGGLELIMNFVLGRSRSVHWALARSMFPVMCLSAFLNNTPCVTFMIPILISWGRRCGVPIKKLLIPLSYASVLGGTCTSIGTSTNLVIVGLQDARYTKAKQLDQAKFQIFDIAPYGVPYALWGFVFILLTQAFLLPGNSSRYAKDLLIAVRVLPSSSVAKKKLKDSGLLQQSGFSVSGIYRDGKYLSKPDPNWVLEPNDILYAAGEFDVVEFVGEEFGLGLVNADAETSAERPFTTGEESVFTPTGGAPYQKLVQATIAPTSDLIGRTVREVSWQGRFGLIPVAIQRGNGREDGRLNDVVLAAGDVLILDTTPFYDEEREDSKNNFAGKVRAVKDGAAKEFVVGVKVKKSSEVVNKTVSAAGLRGIPGLFVLSVDRADGSSVEASDYLYKIQPDDTIWIATDIGAVGFLAKFPGLELVQQEQVDKTGTSILYRHLVQAAVSHKGPIVGKTVRDVRFRTLYNAAVVAVHREGARVPLKVQDIVLQGGDVLLISCHTNWADEHRHDKSFVLLQPVPDSSPPKRSRMVIGVLLATGMVLTQIVGGLKSREYIHLWPAAVLTSALMLLTGCMNADQARKAIYWDVYLTIAAAFGVSAALEGTGVAASFANGIISIGKNLHSDGAALIAIYIATAMLSELLTNNAAGAIMYPIAAIAGDALKISPKETSVAIL",
    "response#3": "PDVTFTVLLAFLTAFDGQIVTVAKAAAGYGNTGLLTVIFLYWVAEGITQTGGLELIMNFVLGRSRSVHWALARSMFPVMCLSAFLNNTPCVTFMIPILISWGRRCGVPIKKLLIPLSYASVLGGTCTSIGTSTNLVIVGLQDARYTKAKQLDQAKFQIFDIAPYGVPYALWGFVFILLTQAFLLPGNSSRYAKDLLIAVRVLPSSSVAKKKLKDSGLLQQSGFSVSGIYRDGKYLSKPDPNWVLEPNDILYAAGEFDVVEFVGEEFGLGLVNADAETSAERPFTTGEESVFTPTGGAPYQKLVQATIAPTSDLIGRTVREVSWQGRFGLIPVAIQRGNGREDGRLNDVVLAAGDVLILDTTPFYDEEREDSKNNFAGKVRAVKDGAAKEFVVGVKVKKSSEVVNKTVSAAGLRGIPGLFVLSVDRADGSSVEASDYLYKIQPDDTIWIATDIGAVGFLAKFPGLELVQQEQVDKTGTSILYRHLVQAAVSHKGPIVGKTVRDVRFRTLYNAAVVAVHREGARVPLKVQDIVLQGGDVLLISCHTNWADEHRHDKSFVLLQPVPDSSPPKRSRMVIGVLLATGMVLTQIVGGLKSREYIHLWPAAVLTSALMLLTGCMNADQARKAIYWDVYLTIAAAFGVSAALEGTGVAASFANGIISIGKNLHSDGAALIAIYIATAMLSELLTNNAAGAIMYPIAAIAGDALKISPKETSVAIS"
  },
  {
    "reference": "MLTVVSLSLSLSRTAPADSPWISTSGSCKNRCFELDEAEPPNCRCDNLCKTYNSCCQDFDEHCLRTEGGFECSKERCGETRNDQHACHCSEDCLAKGDCCTNYKTLCKGDTTWLQDDCEDIRAHECPAGFVRPPLLMVSVDGFRASYMKRGSTVIPNIEKLRACGTHAPYMRPMYPTKTFPNLYTLATGLYPESHGIVGNSMHDPVFDANFNLRGREKLNHRWWGGQPIWITAVKQGVKAGTFFWPLNTPLKEIDKIVGQLMDGLKQMKLHRCVNIIMVGDHGMEEAHCDRTEFLSSYMPSTVDDIILIPGSLGRIRSRYPNNSKYDAKSVVANLTCKKTDQHFKPYLKQHLPKRLHYANNRRIEDIHLLVERKWHIARLYPLYLHPGKCGFAGDHGYDNKINSMQTIFFGYGPMFKFKTKVPVFENIELYNVMCDLLGLKPAPNNGTHGSLNHLLKSPVHKPTMPEEVSKPTISGPIITLTDDLGCSCDDKNKVEELNQRLRQAVDGTKNLPHGRPAVLFRTKYSILHHSDYISGYSESLSMPLWTSYTVSKQVEVTPLPEHLTNCVRPDIRIPPAFSQSCSNYKADKQISYAFLYPPLLAPTQDGKFDAVLITNTVPMYPAFKRIWSHFQKTLVKKYAMERNGVNLICGPIFDYDYDGLRDSAEKIKQHGSGSGPIPTHYYCVITSCLDFTQAEDICTGPLSSSAFILPHRSDNDESCNSSEEESKWVEDLMKLHTARVRDVEILTGLDFYRRTSRSYPEILSLKT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.2. The protein contains novel SMB domains that confer a unique function or activity.3. The designed protein must possess metal ion binding, nucleic acid binding, scavenger receptor activity, polysaccharide binding, phosphoric diester hydrolase activity to support hemangioblast cell differentiation, regulation of angiogenesis, regulation of calcium-mediated signaling under certain conditions.4. The designed protein should have a binding site that is specific to the target Ca(2+), Zn(2+), substrate ligand.5. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "ERVVETRMPTDFGEFRAVGYRSLVDGVEHVALVVGDPASDDGSDVLVRVHSECLTGDVFGSRRCDCGQQLHKSMEIVQAAGRGVVLYMRGHEGRGIGLMHKLEAYHLQDEGADTVDANLALGLPADAREYGTGAQILRDLGVRSMALLTNNPTKRAGLGGYGLEMSRRVG",
    "response#2": "QHQFVSGYSRDFLMPLWTSYTVDRNDSFSAENFSNCLYQDLRISLSPVHKCSFYKNNAELTYGFLYPPQLNKGSNQLYSEALLNTNIVPMYQSFQVIWRYFHGTLLPRYAEERNGVNVVSGPVFDSDYDGCYDSSETLKQNSRLIRNQEILIPTC",
    "response#3": "ERVVETRMPTDFGEFRAVGYRSLVDGVEHVALVVGDPASDDGSDVLVRVHSECLTGDVFGSRRCDCGQQLHKSMEIVQAAGRGVVLYMRGHEGRGIGLMHKLEAYHLQDEGADTVDANLALGLPADAREYGTGAQILRDLGVRSMALLTNNPTKRAGLGGYGLEMSRRVG"
  },
  {
    "reference": "MPHTKDDFPDALYSAAQVRALDAQLIAAGTAGFELMQRAARATWRAMVRRWPDARELTVLAGHGNNAGDGYLVATLARRAGWSVRVLTVGEPRRLQGDAAIAHAEAVAVGVPMEPWSDAAELRGVLLDALLGTGLSGDVREPYVQAIDTINVSGLPVAAVDIPSGLCADTGRVLGTAVAADLTVTFIGLKLGLFTGEAADRVGELVFNDLHADPDIVEAAPASARLLLPHNLPCLTPRAATAHKGKFGHVLLIGGDRGFGGAIQMSTESALRCGAGMVSMATRSEHVSAALTRLPEVMVQGTHSANQLMGLLHQASVVVVGPGLGQAAWGRSLLSAAANASLPQVWDADALNLLSRGDVGLPERCVITPHPGEAARLLEISTAQVQADRPAAAHALSQKYTATVVLKGAGSLIASPDGRLAVCSQGHPAMATAGLGDVLAGVVGALLAQGMEGFEAACLAVWLHASAGAQAGRSGRGLAATDLIPAIRQLLEEHSPCLK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with kinase activity, NADHX epimerase activity, ATP binding, NADPHX epimerase activity, metal ion binding is needed to enhance nicotinamide nucleotide metabolic process in a specific context.2. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration3. The protein contains novel YjeF N-terminal domains that confer a unique function or activity.4. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "MLRPLYTADEMRAAEQGHDVEAMMERAGSAVAETVLRRYPDARRIAAVCGKGANGGDGRIALRVLAEAGRETGEELDGADVVIDALFGTGFHGAPRDDAAGRIEQINGAGVPIVAVDLPSGVDASTGEVVGAVVDADVTVTMHGPKVGLEVGPGRFHAGEVEVAEIGLGPGDVRLVLRRREKSSHKGDNGRVLIVGGGPFAGAPALSALASLRTGADWVTVAAPRNVASIIASISPNLIVQPLSGSGSGSILTEDDVSLVSSLMQMHDVLVIGMGLGAAEETKRAVKLIIEDEAVKKVVVDADGLYGLDLPLKVSKLKSKLKSKQVIITPHAGEFSKMDAQRPPPDGEERLGFIQEFSLRNRVVTLLKAAHDVISDGKRIKVNATGNAGMTVGGTGDVLSGITGALFATSEDAFEVATAAAFISGSAGDAAFRDKGLSLLATDVVEAIPGILRARALPQRPGNAHKNQCGRVLVVAGSTGYTGAAALCARAAQKSGAGLVSLCIPRSLDNILEIKLTEEITCPLADGNKGYLTEKNADAILALAGEYQVLAIGPGLGRNASTAAMVRKVVARSPVPVVLDADGINAYEKAGHLLKKAKSPVIITPHDGELKRLTGEKEIGWGPARIRALRNIQKELGITIVLKGGPTLVVSSTCMVNVNTTGNPGMATAGAGDVLTGLISGLYAQTHNLEQAVLTGVYLHGLAGDYGAMACTEYCLTAGDIITFFPQGRGDLLPTLQSRGKRFHKGQFGRMLVLGGSKNYTGAPSFSSLTGINFGLDLVITYTPQIIGDVIRSYSPNLIVRSQPGDWLSKQNWEEIIWLVEWSNAILIGPGMGEEKETEALLIDLLKKFREDKKAFVLDADALKLVKNHHDLLKRQSCILTPHEGELKIMTGAELPPYDDIEARSESIIKLAKELNCVLLVKGPYDYISDGEELRINRTGCQEMAVGGTGDVLAGLCASFLATGNKIFESACSAAFLNGYIGEFCKQKVGTRFNAMDMIDKINEAISELE",
    "response#2": "MRDIENRIFAGGMPVAALMEKVAGLIADRIQTKLAEDTTYFPHSPSFRIGILVGPGHNGGDALVVARELYFRGYEVGIYCPFSKRKELTSQHLQYAQSIGIICYESIELLADCHLFIDGLFGFGLERAISDPIATAINQLNEWHKPVIAIDLPSGLHTDTGEVLGTAVRATHTLCLGLWKLGLLQDQALDCVGKAELIDFDIPLADIAPVLWQELLRHEDLGTHKYARGHLTVLGGATATGAARLAALAGRRAGAGLVTIATPHEAMAVYQAADPGNLIAEVEDGEGFGRLLDDKRRNAILVGPGSGLNERTRTSAVAALATGRPVVLDADAITVFTENPASLFDLIRGPVLLTPHEGEFRRLFPALSAVSGKVERTRRAARQSGATVLLKGPDTVIAAPDGRAVINVHAPATLATAGSGDVLAGMAAGLAARGLSPLAAGAAAAWLHGDCAFRFRRPGLIAEDLIGCIPEALAARALPQRPGNAHKNQCGRVLVVAGSTGYTGAAALCARAAQKSGAGLVSLCIPRSLDNILEIKLTEEITCPLADGNKGYLTEKNADAILALAGEYQVLAIGPGLGRNASTAAMVRKVVARSPVPVVLDADGINAYEKAGHLLKKAKSPVIITPHDGELKRLTGEKEIGWGPARIRALRNIQKELGITIVLKGGPTLVVSSTCMVNVNTTGNPGMATAGAGDVLTGLISGLYAQTHNLEQAVLTGVYLHGLAGDYGAMACTEYCLTAGDIITFFPQLLLICGSRRYSGGALLTGLGGRASGVGMLSIAVPESLKSIMVAQLPEALIIGCPETESGAIAQLQLPENTDLSKFNAIACGPGLTRDANPILQQVLESTVPLVLDADGLNILADMGTIPTLQKRQAPTVLTPHAGEFKRLFPDLSDANQDRVKAVREAAAQSHAVVLLKGARTAIANPQGTVWLNPESTPALARGGSGDVLTGLLGGLLAQASSKNIPVEDITATAAWWHAQAGILAAQERTELGVDAHTLTQY",
    "response#3": "MRDIENRIFAGGMPVAALMEKVAGLIADRIQTKLAEDTTYFPHSPSFRIGILVGPGHNGGDALVVARELYFRGYEVGIYCPFSKRKELTSQHLQYAQSIGIICYESIELLADCHLFIDGLFGFGLERAISDPIATAINQLNEWHKPVIAIDLPSGLHTDTGEVLGTAVRATHTLCLGLWKLGLLQDQALDCVGKAELIDFDIPLADIAPVLWQELLRHEDLGTHKYARGHLTVLGGATATGAARLAALAGRRAGAGLVTIATPHEAMAVYQAADPGNLIAEVEDGEGFGRLLDDKRRNAILVGPGSGLNERTRTSAVAALATGRPVVLDADAITVFTENPASLFDLIRGPVLLTPHEGEFRRLFPALSAVSGKVERTRRAARQSGATVLLKGPDTVIAAPDGRAVINVHAPATLATAGSGDVLAGMAAGLAARGLSPLAAGAAAAWLHGDCAFRFRRPGLIAEDLIGCIPEALAARALPQRPGNAHKNQCGRVLVVAGSTGYTGAAALCARAAQKSGAGLVSLCIPRSLDNILEIKLTEEITCPLADGNKGYLTEKNADAILALAGEYQVLAIGPGLGRNASTAAMVRKVVARSPVPVVLDADGINAYEKAGHLLKKAKSPVIITPHDGELKRLTGEKEIGWGPARIRALRNIQKELGITIVLKGGPTLVVSSTCMVNVNTTGNPGMATAGAGDVLTGLISGLYAQTHNLEQAVLTGVYLHGLAGDYGAMACTEYCLTAGDIITFFPQLIVAGSSGKTGAAALSANSAVRAGSGLVTLAVPESLNPILEVKTTEAMTVPLADSGGGYLTDSAFPAIAGLLPDKDALAIGPGLGQHPATVTLVRALLKAADLPLVIDADGLNAVAQNANCLLDKKSTHVVLTPHPGEMARLMGATIADVEADRIATARKFAAAFGVHLILKGSRTIIAAPDGSTAINGSGNPGMASGGMGDVLTGIIVSLLGQGYNCHDACRLGVFIHGYAADLVAQDKGEIGMCAGDVQEKL"
  },
  {
    "reference": "MSGTYDDSVGVEVSSDSFWEVGNYKRTVKRIDDGHRLCNDLMNCIHERARIEKVYAQQLTEWAKRWKQLVERGPQYGTVEKAWHNLMTEAEKVSELHLEVKNALMNEDFEKIKNWQKEAFHKQMMGGFKETKEADDGFRKAQKPWAKKLKEVEAAKKSYHAACKEEKLATSRETNSKADPAMNPEQLKKLQDKVEKSKQDSQKTKEKYEKSLKDLDGTTPQYMENMEQVFEQCQQFEDKRLSFFREVLLEVEKHLDLSNVESYASIYRELEYAIKSADAMEDLKWFRNNHGPGMSMNWPQFEDWSADLNRTLSRREKKKPTDGVTLTGISQSGEQSSIQNQHSSHLSVQSAQSTNNPFEDEEETVSINETENKKIENVGSYEKTHPAEWSDDESNNPFNPSDTNGDNNPFDEDALTTLEVRVRALYDYDGQELDELSFKAGEELTKIEDEDEQGWCKGRLEGGQVGLYPANYVESVQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must contain a signal peptide for proper functionality.2. The designed protein should contain one or more SH3, F-BAR domains that are essential for its function.3. The designed protein should be involved in caveola assembly, plasma membrane tubulation by localizing to cytoskeleton and exhibiting phosphatidic acid binding.4. The designed protein should contain at least one NPF1 motif to achieve its intended function.5. The protein must exhibit the following characteristics: Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds; Plays a role in intracellular vesicle-mediated transport; Regulates the morphogenesis and endocytosis of caveolae . The designed protein sequence is ",
    "response#1": "FTKAVSRAPQSFRQKFKMGEQTEDPVYEDAERRFQELEQETKKLSEESKRYSTAVNGMLTHQIGFAKSMEEIFKPISGKMSDPNATIPEDNPQGIEASEQYRAIVAELQETLKPDLALVEEKIVTPCQELLKIITYIRKMATKRNHKKLDLDRHLNTYNKHEKKKEPTAKDEERLYKAQAQVEVAQQEYDYYNDLLKTQLPILFSLEAEFVKPLFVSFYFMQLNIFYTLYNRLQDMKIPYQD",
    "response#2": "LPVAVALTESVNAYFKGADPTKCIVKITGDMTMSFPSGIIKVFTSNPTPAVLCFRVKNISRLEQILPNAQLVFSDPSQCDSNTKDFWMNMQAVTVYLKKLSEQNPAASYYNVDVLKYQVSSNGIQSTPLNLATYWKCSASTTDLRVDYKYNPEAMVAPSVLSNIQVVVPVDGGVTNMQSLPPAIWNAEQMKAFWKLSSISEKSENGGSGSLRAKFDLSEGPSKPR",
    "response#3": "IEDIKLSKKSWVFPWQSCTVFDVSPVNYTFKVQAMSAEKLPFVLPAVFTIGPRVDDDDALILYARLISPHDKDSNHVHELVEGVIEGETRVLAASMTMEEIFKGTKEFKKEVFDKVQLELNQFGLVIYNANVKQLVDVPGHEYFSYLGQKTQMEAANQARIDVSEAAA"
  },
  {
    "reference": "MKVRASVKSICKDCKVIRRNGSVRVICKNPRHKQRQG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The ideal protein would be one that plays a critical role in translation through structural constituent of ribosome within ribonucleoprotein complex, ribosome. The designed protein sequence is ",
    "response#1": "IGCRVTLRRKAMYEFFDRFVSIAVPRIRDFRGLPDTSFDGRGNYTVGVREQIIFPEIDIDKVPRIQGMDISFVTSATTDEEAFVLLTELGMPG",
    "response#2": "MQVKFYLVEGRMLIRHDRMPEWWKFRKYVRALKPEHAVEKVLSELGSNHKVKRYHVKIERI",
    "response#3": "IETTVPKAKAMRPHVEKMITLGQ"
  },
  {
    "reference": "MVSDSNRPAQPPRTPLATVAAWAGAELRGNPAGLEVSGISLSSQRIRPGDLYAALPGSRAHGIEFAQDALAAGAVAVFTDEAGADRGPAGAPHLVVDRPRAVLGRLSARIYGEPAASMRMVGVTGTQGKTTTTRLLEAGLQQAGVPAAVVGTVGTRIAGEEVRSSLTTPEAPDLHALFAVMRERDVRACAMEVSSHALVMGRVDGVVFDVAVFLNLGRDHLDFHRDLEDYYAAKASLFTPERARFALVNVDDAHGRRLAEEATVPVRTFSMGRGDDAQRADWRAVDVRLRATGSEFVVVAPDGRRIPAGCALPGDFNVANTLAAVAAAAEAGLDPAVVAAGIAAAGGVPGRLERVDAGQDFLVVVDYAHKPDAVEAAIRTLRPLTEGRVIVVLGAGGDRDPGKRPIMAGIAARLADLLVVTDDNPRTEDPAAIRAAMLAGVVTGQAIEVGGRRAAIREALRRAGPGDIVLIAGKGHETGQEVDGVVHPFDDREVVREEIVACSR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein's functional design necessitates the inclusion of a Meso-diaminopimelate recognition motif motif.2. The presence of Mg(2+) is necessary for the protein to function in the desired environment.3. A protein that contributes to peptidoglycan biosynthetic process, cell wall organization, regulation of cell shape, cell division, cell cycle by functioning in cytoplasm with UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, ATP binding, magnesium ion binding.4. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..5. We require a protein that can specifically bind to the metabolites produced in the Cell wall biogenesis, peptidoglycan biosynthesis pathway.6. The UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate, ATP binding site should be stable and able to withstand changes in environmental conditions.7. The designed protein must have at least one Mur ligase C-terminal, Mur ligase central, Mur ligase N-terminal catalytic domain. The designed protein sequence is ",
    "response#1": "MNLKELVANLAPDIAIQGLAVDSRAVKPGDVFFALAGAKTDGARFVAAAIEAGAVAVAGDHPEGIGLAVDSRAVKPGDVFFALAGAKTDGARFVAAAIEAGAVAVAGDHPGAAEAWGIAVPDPRRALAELAARFYGHPSADLWMIGITGTNGKTTIATLLFKLFRELGYKVGLISTVENQINDQIIPATHTTPNPIALNQLLRQMVTEGCQFAFMEVSSHAVVQHRIEGVSFVGGVFSNITHDHLDFHKTFDNYIKAKRAFFDALPKTAFALTNGDDKNGLVMLQNTKAGRKTYALKQLADFKGRIIENKFSGLNLEVDDVDVFFKLVGSFNAYNLLAVYGAALALGHDADAVLEAMADLEPVPGRLELVGERNGAPVFVDYAHKPDALAKSLQALRPYAKRKLVVVFGAGGDRDAGKRPLMGAIAAESADAVIVTDDNPRREDPATIRAAILAAAPGAREIGDRAEAIRTAIGELQPGDALVIAGKGHEAYQEIAGVRHPFDDVAVAREALERREAAV",
    "response#2": "MKLRDLLAPFVPLAGAEPVTIAGVAIDSRRVQPGALFLAYQGTRSHGLDHVDAAIAAGAAAVAWDDAQPPVLVVADTRHALALLAARFYGAQPETMVAVTGTNGKTTIATLLYDLFKKLGYKVGLLSTVCNYVDEKEIKATHTTPDALQLNALLAEMVEAGCEYCFMEVSSHAIDQKRVGALNYKGGIFTNITHDHLDYHKTFDAYLKVKKAFFDQMGKKSFAITNADDKNGKLMLQNMKGHKFTYSTRSFADYRCQILEKHFTGMLLEMDGTEIWTNFIGDFNAHNLLAVYGCALQLGLDEAQVLGALSALTGAEGRLELVGERNGAPVFVDYAHKPDALAKSLQALRPYAKRKLVVVFGAGGDRDAGKRPLMGAIAAESADAVIVTDDNPRREDPATIRAAILAAAPGAREIGDRAEAIRTAIGELQPGDALVIAGKGHETYQIIGTTKLHFDDREEAAAALAAR",
    "response#3": "MKLKDILKNIKGIRIYNKNEDVNILIAGVAIDSRRVQPGALFLAYQGTRSHGLDHVDAAIAAGAAAVAWDDAQPPVLIIENAKGIMSVISANFYKNPAKKLKLIGLVGTNGKTSTVLMIKNILCDAGINCGMISTVTCNILGKTINAKNTTPDPIELQKIFSEMESKGVDYVVMEVSSHSIAQGRIKDSVFKIGIFTNLSEDHIGPNEHKDFEDYKNCKGLLFKQAYLGIVNIDDEYGKRLYEMTEGKKFTYGIDNDADIKAKDIILKKGGIKFILDYNGLETEIKSPLIGKHNVYNALAAISACLLEGIDLDTIKRGLEKVEVPGRMQRVPSNEGCFIVDYAHTPDALQQVLASLKTHCDGQLWAVFGCGGDRDAGKRPLMAQAGLAGADQIILTADNPRTEDPNIILQDMQAGMSAEQYQRTHIEPARQKAIEYAVRQAAAADIVVIAGKGHENYQEINGVKHHFDDKEIAKEILKSL"
  },
  {
    "reference": "MTTSTPDIQPAVQHTAQVAIVGAGPVGLMMANYLGQMGISVLVVEKLATLIDYPRAIGIDDESLRAMQAVGLVNDVLPHTTPWHAMRFLTPKGRCFADIQPMTDEFGWSRRNAFIQPQVDAVMYHGLQRFPQVRCLFSREVGAFSQTGDSVTLNLKGPDGERETVRADWLVACDGGASFIRRTLNIPFEGKTAPNQWIVIDIANDPLATPHVYLCCDPVRPYVSAALPHGVRRFEFMVMPGETEAQLSEPHNMRRLLSKVLPDPDRVELIRQRVYTHNARLAERFRINRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVVNGKAGEALLDSYQQERRDHAKAMIDLSVTAGHVLAPPKRWQGAVRDGLSWLLNYLPPVKRYFLEMRFKPMPQYREGALLTDGAGKTSPVGKMFIQPQVTLESGESVLLDEVIGANFAIIGWGCNPQWGLNAGQIARWRAIGVRFIQVVPEVQIHREQDNAPGTLRVGDTQNRLKSWFALHNTAIAVVRPDRFVAALAIPQTLGAQLTALAEKMTLATGDTAHAEEKVA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be optimized for 3-(3-hydroxyphenyl)propionate hydroxylase activity activity in order to improve 3-(3-hydroxy)phenylpropionate catabolic process.2. The protein must exhibit the following characteristics: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3-hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI)..3. The protein should be optimized for activity in Aromatic compound metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "CCMSHSPLLNLPGPAQELLDEIDKAIAAARDFVAEFDPELVVTFSPDHYNGFFYRAMPPFCIGTAAEGVGDYGTHEGPLDVPSDLATDCARAVLDHDVDVALSAAMDVDHGTVQPLQKLFGDATAKPVIPVFINSVATPLGPIRRVRALGAAVGAHLATLGKRVLVIGSGGLSHDPPVPTLATAPPAALDRIVRGVPMTTDQRQARQTAVIEAAREFASGRGTLAPLNPDWDRAFLDIVDSGRLAEVDGWDNGWIAEQAGNSAHEVRTWIAAFAALAAQGEYVTENRFYRAAPELIAGEL",
    "response#2": "CTMSHSPLMGRNDPAQTVIDDVDAAFENARTFIADFAPDLIVIFAPDHYNGVYYDLMPPFCIGAAAQSVGDYGTESGPLNVDRDAAYTVAREVLASGVDVAFSERMHVDHGFAQALQLLVGSITAVPTVPIFINSVAEPLGPVSRVRLLGEAVGRAAANLDKRVLFVGSGGLSHDPPVPQFATAPTEVKEKLIDGRNPTEAERNAREQRVIDAGRDFAAGVATIAPLNPEWDRNLLDVLTSGEIEQIDSWTNEWFVEQAGHSSHEVRTWIAAYAAMSAAGKYRVTSTFYREIPEWIAGFG",
    "response#3": "CTMSHSPLMGRNDPAQTVIDDVDAAFENARTFIADFAPDLIVIFAPDHYNGVYYDLMPPFCIGAAAQSVGDYGTESGPLNVDRDAAYTVAREVLASGVDVAFSERMHVDHGFAQALQLLVGSITAVPTVPIFINSVAEPLGPVSRVRLLGEAVGRAAANLDKRVLFVGSGGLSHDPPVPQFATAPTEVKEKLIDGRNPTEAERNAREQRVIDAGRDFAAGVATIAPLNPEWDRNLLDVLTSGEIEQIDSWTNEWFVEQAGHSSHEVRTWIAAYAAMSAAGKYRVTSTFYREIPEWIAGFS"
  },
  {
    "reference": "MSLVGAFPTPVVHHEGYPFAAAAAAAAAAAASPDHEENPYFPRLGSIGHPEMSPPTAWALSTVPEYASGARLDHSHYGEECRPGAGPPGLGGRRPVEASGHRQRKERRQTQSNSALAELRECIPNVPADTKLSKIKTLRLATSYIAYLMDLLAKDDQNGEAEAFKAEIKKTDVKEEKRKKELNEILKSTVSSNDKKTKGRTGWPQHVWALELKQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The ideal protein would be one that plays a critical role in positive regulation of transcription regulatory region DNA binding, negative regulation of osteoblast differentiation through RNA polymerase II-specific DNA-binding transcription factor binding, DNA-binding transcription activator activity, RNA polymerase II-specific within chromatin, transcription regulator complex. The designed protein sequence is ",
    "response#1": "GIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDMC",
    "response#2": "GIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDMC",
    "response#3": "GIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDMQ"
  },
  {
    "reference": "MEQHTIITIDGPAGVGKSTLAKKLGTILNLPYLDTGAMFRKLALQLGNKAETLPDSILQEQCKKVTFQLQGVGKNSLLMCNGESIGHEIRSETAGILAAQLGERTIIREYLKNIEQQIGNTMSIIAEGRDLGTEVFPKAQFKFFIDANPIIRAQRRFNQLKKEGIFQDYNDILHSINYRDKLDKNRTIAPLEPAKDAILIDSSIMDIDSILKIMLNYITIPHLSQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The desired protein should be able to perform ATP + dCMP = ADP + dCDP2. The ideal protein would be one that plays a critical role in pyrimidine nucleotide metabolic process through dCMP kinase activity, ATP binding, CMP kinase activity within cytoplasm. The designed protein sequence is ",
    "response#1": "IAIDGPAGAGKSTVAKKLAKLLGFTYIDTGAMYRAITLKVLRENISLEDEERIVEVARKSDISLDGERVFLDGEDVSEEIRKPIISQKVSVVSQIPEVREILVKKQRKIAEGKNVVMDGRDIGTVVLPDAQFKFFLTASLEERARRRYEELKNKGTEVKYEEVLEEIKKRDSLDSGRKTSPLTIPEGAILIDTTDLTEEEVVERVYEAIAAGA",
    "response#2": "VIAVSGQPGSGKTTIAREIARVLGLPLVSSGLLFREMAARMGMDFIEFHKYAETNPDIDKKVDSLAIERAKAGDVVLEGHLTAWIVRPYADVCIYLKASLETRARRVALRDGKSLQDALREVAEREELNRRRYLSIYGIDINDLSIFDLVLDTR",
    "response#3": "VTIDGVAASGKSSVSSGVARALGIPYVSSGLLYRAATLLGLEAALDLSDAPRLLAHLRALPLRLEPLAEGNRVWSGERDLTPGLHQSAVDAGVSTVAAHPELRAWVDEQLRQLPPPFVAEGRDMGTNVFPDAPAKFYLTASPRIRAERRSAERPEDVDAIEAALIARDRKDAAQSAPAPDARVIDTGPLGLEEVIGEILGEL"
  },
  {
    "reference": "MLSGKNVLLAVTGSIAAYKSAFLIREFIKSGAFVKVIVTEAALQFVTTVTLSTLSKNPVYKDFIKDENTGEWNNHVDLGSWADLMVIAPATANTISNLVSGKADSFFLSTFLSCNAPVFVAPAMDLDMFKNKSTQSNIQSLTKRLVNVIEPKEGELASGFEGKGRMEEPEEIVAFVNNYFLEKATLKNLNIIISAGPTYESIDPVRFISNYSTGKMGYALANEAQKRGANVQLISGPVSLKTPENVQRIDVKSAQEMKKEISKRFDKIDVLIMSAAVADYEPKNTASYKIKKQDQEISINLKKTPDILKDLTKSKKKQIIIGFALETENGIENAYEKMKRKNLDAIILNSLSDIGAGFGYDTNKITLITPTEKREFELKSKDLVAIDILNFVEECI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal domain. The designed protein sequence is ",
    "response#1": "MRILLGITGGIAAYKAANLIRGFSELGHEVTAVPTENALRFIGKPTLEALSGHPVETDMYVDVDMVRHVELGQQADLIVIAPATASFIARLAAGIADDLLLNAVLASKAPVVLAPAMHTEMWQNSATRANIETLAQRGIRVLEPSSGRLTGADSGPGRLPETNEIISR",
    "response#2": "MRIILGVGGGIAAYKSASLLRLFKEAGHDVTVMPTDAAEKFVGRATWEALSGNPVSNSVFDDVESVNHVRLGQEADLVVVCPATADLLARAAAGLAGDLLTNTLLVATAPVVFVPAMHTEMWQHPATVANVATLRSRGSTVMDPAVGRLTGKDTGPGRLPEPADIYG",
    "response#3": "HILLIIGGGIAAYKSLELIRLLTRAGHEVTPVMTESAAQFVTPLSVSALAGKKVHEALFDLTAEAEMGHIQLSRVADLVVVAPATADLMARMAQGRASDLASTLLLATDTPVLIAPAMNVRMWEHPATRRNLETLKSDGIMVVGPTEGEMACGEYGPGRLAEPAAILAAILG"
  },
  {
    "reference": "MYYCGLNDSVSNAWVNHGFGLCFLHTLGSSIIGLWILTFGCIQLGFYRKYATRLSAAVLPQNKKFKIQVFGHFALTSLAVLSVIVRHAVSHDQLFGYEILDLVANLIIWPLTLTVLLVERNFQLPSAPSNSHGVVLISTWTLAFLWQNLKLLSLNNEPFFYDLSHLREQIELTLFCFEYAFVFSAFFLGMKAPGISQTHDYLFGEHSDVEAVLPDGSTRNQDIQQSTWSGLWTKLGVLLPYMWPKKSLGLQFRVFMCFLLLAGVRVANVYVPIYYKKIVDTLTPVALGAEVRFCWDLIAIFVALKLIQGGGTGGQGILNNIRTFLWIRVQQYTTREIQVGLFTHLHHLSLRWHLSRKTGEVLRIMDRGTSSINGLLSYLVFNILPTIVDIVVAIVYFSSAFNIWFGLIVLVTMVIYLALTVAVTEWRTKFRRSMNQADNEQRSKGVDSLLNFETVKYYGAEHYEIDRYEKAILSFQTEEFKSLTSLTLLNGTQSLIINGGLLVGSLYCALLVTKGYLTPGDYVLFGTYILQLMVPLNFLGTLYRVIQESFINMENMLELMDEPQEISDLPNALPIMVPQGKIEFKNVNFHYNPERPILKDVGATGSGKSTIVRLLFRFYDVQSGAVLIDDQNVQNVTQASVRNAIGVVPQDTVLFNDTISEVENAARHADIHDKIIAFPDGYDTKVGERGLKLSGGEKQRVAIARTILKAPKIVLLDEATSALDTQTERHIQNALQEVSVDRTTIVVAHRLSTITNAHCILVINEGEI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a specific function within the extracellular region.2. The presence of ABC transmembrane type-1 domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "KNKVFRSFVIALATAPIWSSFLIKLIGLKTLLDLVLGLALNRVGDNNLTFGSGYTLIGMIYLFTPFMFLPLYNNFCILPKNLILASQDLGYNWITSFIKVVIPFSKTAILSGIALTFFPSLTSVAIAQFLDNSNQNNTLGNYVFTLGNNGYDSAIERGRASGAIIIAALITFAFYFVVIFAPRM",
    "response#2": "FWNSTVITVLSVLVVMLFIPAAAYSIARNMSRRKAFNIMYSLLILGIFVPFQVIMIPITVMMSKLGLANMWGLIILYLTYAIPQTLFLYVGYIKLSVPDSLDEAAEIDGADKLTTYRKIIFPMLKPMHATTLIINALWFWNDFMLPLLILNKDSSMWTLPLFQYNYSGQYFNDYGPSFASYIVGIITITIVYLIM",
    "response#3": "FMVTALSSITALVLAVPVAVGIAVFLTHYAPRRLSRPFGAMVDLLAAVPSIIFGLWGIFVLAPKLEPIARFLNRNLGWLFLFKQGNVSLAGGGTIFTAGIVLSVMILPIVTSISREVFRQTPLIQIEAALALGATKWEVVRMTVLPYGRSGVVAASMLGLGRALGETVAVLVILRSAARPGTWSLFDGGYTFASKIASAASEFSEPLPTGAYISAGFALFVLTFLVNAAATNSVVAATLSTLVAVAFGVPLAYWLSRTSFRGRDVILALVMLPLVLPPVVSGMLLLRLVGPAGLGQLTSVPLTRSLFGVVLAQTYVASPFLVVTAKTAFDGVDRQLEAAARSLGEDRVGSVRRVTLPLAKQGILAGVTLTFARAIGEFGATLMLAYYPRTLPVQIWVSYLSTGLDAAFPVALVLVGIAVGAILFSNYFFN"
  },
  {
    "reference": "MQMPAGPDKKCNLLGLDRSGLERFFVDIGEKPFRARQLLQWIHQRGATCFSEMTDLSLALRARLAEVAVITPPVIQHEQRSADGTIKWLMTLAGGNAVETVFIPEPGRGTLCISSQIGCMLNCTFCSTATQGFSRNLTADEIIGQVWLAVQALAHDADDARRITNVVFMGMGEPMLNLPAVRPALSLLRDDLAYGLAARRVTISTAGVVPGIDEMASGEEFALAVSLHAADNELRSRLVPLNRKYPVEELISACQRFVRARSRRSITFEYTMIDGVNDQPEHARELVRRLSCLPCKINLIPFNPFPGTPFRASDDSAIERFQSTTRAAGLITTVRKTRGDDIDAACGQLVGRLLSPSQRRLLVQQQLARQAVAS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein's S-methylcysteine intermediate, Proton acceptor must be carefully engineered to ensure that the amino acids involved in adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine are properly positioned for optimal catalysis.2. The designed protein should be involved in rRNA base methylation by localizing to cytoplasm and exhibiting tRNA binding, tRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding, rRNA binding.3. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.4. The designed protein must have at least one Radical SAM core domain.5. The designed protein should have a binding site that is specific to the target S-adenosyl-L-methionine, [4Fe-4S] cluster ligand. The designed protein sequence is ",
    "response#1": "MPPKHFADLSEDARIEALAELGLPKFRADQIARHYYVRHEADPRTMTDLPAAAREEVAEKLFPQLMQPLRHLTTDDGETRKTLWRLHDATLLESVLMRYPGRATLCVSSQAGCGMNCPFCATGQGGLDRNLSTGEIVDQVRNAAATMAAEGKRLSNVVFMGMGEPLANYKRVVSAVRQITAPGPHGFGISQRGVTVSTVGLAPAIRRLADEELSVRLAVSLHTPDDELRDTLVPVNNRWPVAEVLDAARYYADKSGRRVSIEYALIRDKNDQPWRADMLGKKLHKALGPKVHVNVIPLNPTPGSEWDASPKERQDEFVRRVIAQGVPCTVRDTKGSEIAAACGQLKADEQVSAVNR",
    "response#2": "MPPKHFADLSEDARIEALAELGLPKFRADQIARHYYVRHEADPRTMTDLPAAAREEVAEKLFPQLMQPLRHLTTDDGETRKTLWRLHDATLLESVLMRYPGRATLCVSSQAGCGMNCPFCATGQGGLDRNLSTGEIVDQVRNAAATMAAEGKRLSNVVFMGMGEPLANYKRVVSAVRQITAPGPHGFGISQRGVTVSTVGLAPAIRRLADEELSVRLAVSLHTPDDELRDTLVPVNNRWPVAEVLDAARYYADKSGRRVSIEYALIRDKNDQPWRADMLGKKLHKALGPKVHVNVIPLNPTPGSEWDASPKERQDEFVRRVIAQGVPCTVRDTKGSEIAAACGQLKADEQLA",
    "response#3": "MPPKHFADLSEDARIEALAELGLPKFRADQIARHYYVRHEADPRTMTDLPAAAREEVAEKLFPQLMQPLRHLTTDDGETRKTLWRLHDATLLESVLMRYPGRATLCVSSQAGCGMNCPFCATGQGGLDRNLSTGEIVDQVRNAAATMAAEGKRLSNVVFMGMGEPLANYKRVVSAVRQITAPGPHGFGISQRGVTVSTVGLAPAIRRLADEELSVRLAVSLHTPDDELRDTLVPVNNRWPVAEVLDAARYYADKSGRRVSIEYALIRDKNDQPWRADMLGKKLHKALGPKVHVNVIPLNPTPGSEWDASPKERQDEFVRRVIAQGVPCTVRDTKGSEIAAACGQLKADEQIRG"
  },
  {
    "reference": "MSFLPGMTPVTLSNFSWALEDRMLEGNSTTTPTRQLMPLVVVLSSVSLVTVALNLLVLYAVRSERKLHTVGNLYIVSLSVADLIVGAVVMPMSILYLHRSAWILGRPLCLFWLSMDYVASTASIFSVFILCIDRYRSVQQPLRYLRYRTKTRASATILGAWLLSFLWVIPILGWHHFMAPTSEPREKKCETDFYDVTWFKVMTAIINFYLPTLLMLWFYIRIYKAVRRHCQHRQLINSSLPSFSEMKLKLENAKVDTRRMGKESPWEDPKRCSKDASGVHTPMPSSQHLVDMPCAAVLSEDEGGEVGTRQMPMLAVGDGRCCEALNHMHSQLELSGQSRATHSISARPEEWTVVDGQSFPITDSDTSTEAAPMGGQPRSGSNSGLDYIKFTWRRLRSHSRQYTSGLHLNRERKAAKQLGCIMAAFILCWIPYFVFFMVIAFCKSCSNEPVHMFTIWLGYLNSTLNPLIYPLCNENFRKTFKRILRIPP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that can perform calcium ion transmembrane transporter activity, histamine receptor activity in plasma membrane to support manganese ion transmembrane transport, inositol phosphate-mediated signaling, calcium ion import, positive regulation of inositol trisphosphate biosynthetic process.2. The designed protein should contain Extracellular topological domains.3. The designed protein should have at least Helical; Name=2, Helical; Name=1, Helical; Name=3, Helical; Name=4 transmembrane domains to ensure proper localization.4. The protein must exhibit the following characteristics: In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system.. The designed protein sequence is ",
    "response#1": "GNAILIKVFFKTKSMQTVPNIFITSLAFGDLLLLLTCVPVDATHYLAEGWLFGRIGCKVLSFIRLTSVGVSVFTLTILSADRYKAVVKPLERQPSNAILKTCVKAGCVWIVSMIFALPEAIFSNVYTFRDPNKNMTFESCTSYPVSKKLLQEIHSLLCFLVFYIIPLSIISVYYSLIARTLYKSTLNIPTEEQSHARKQIESRKRIARTVLVLVALFALCWLPNHLLYLYHSFTSQTYVDPSAMHFIFTIFSRVLAFSNSCVNPFAIYWLSKHFHRCFKEVLVSAAAISGLLFGYDTAAISGMLVIIKDDLGTILSSWQKEVITSATTLGALLGGLAAGCVSDFTGRRLVIVFANVAFIGGSICQAACHTVAAMIAGRFIVGLGVGLASCIVPLYIGELAPTMIRGRLVTINCVAVTLGQVVAYAIGASFQNVHNGWRWIVGLGAMPSFVQLAAIGFLPESPRILLLRSDVAGARAITAKIYPLATIEQVDRKIEIMKAAVDQSIEYNANSTWFERLKSLVMVGTNRRALIIGCGLQAAQQLCGFNTLMYYSATIFAMLGFNNATAVGLIVATVNVLFTLVALKIVDPVGRRRTMLFTLPIMILALVFAAIFFYYLTLSTNGILIEDHDYPRSLSILVLLSMLLYVAGYATGLGNIPWQQGELFRLEVRGIGTSICTAVNWSCNMLIAGTFLSLMDAATPSGAFGIYAGFCVIGWVFCWMLYPETKNRTYEEISQMFQG",
    "response#2": "GNVYTLVVMCRFLRASASMYVYVVNLALADLLYLLSIPFIIATYVTKDWHFGDVGCRVLFSLDFLTMHASIFTLTIMSSERYAAVLRPLDTVQRSKGYRKLLVLGTWLLALLLTLPMMLAIQLVRRGSKSLCLPAWGPRAHRTYLTLLFGTSIVGPGLVIGLLYVRLARAYWLSQQASFKQTRRLPNPRVLYLILGIVLLFWACFLPFWLWQLLAQYHEAMPLTPETARIVNYLTTCLTYGNSCINPFLYAFLGRNFRSHLLSAVAGISGLLFGYDTGVISGALAVLGSDLGHVLSSGQKELITSATSFAALISATTSGWLADWVGRKRLLLCADAIFVIGSVIMAASRNVAMMVVGRFIVGYGIGLTSLIVPMYITELAPARLRGRLVIIYVVFITGGQLIAYSLNAAFEHVHQGWRIMFGIGAAPALGQLISLFWTPESPRYLLRHNHVEKVYKILSRIHPEAKPAEIAYKVSLIQEGVKVDFPEGNKFQHFFHSLKVLFTVPSNRRSLFIGCFLQWFQQFSGTNAIQYFSAIIFQSVGFKNSISVSIVVGATNFVFTIVAFMFIDRIGRRRILLCTSAVMIAGLALCAIAYHFLPADTTQNTNSGWQYVVLASIIIFLASYASGIGNIPWQQAELFPMEVRALGAGFSTAINWVGNLIISASFLTMMESITPTGTFALFAGFCFVGLVTSYFTYPELHNKEIMTKIRKK",
    "response#3": "LVSAAAISGLLFGYDTAAISGMLVIIKDDLGTILSSWQKEVITSATTLGALLGGLAAGCVSDFTGRRLVIVFANVAFIGGSICQAACHTVAAMIAGRFIVGLGVGLASCIVPLYIGELAPTMIRGRLVTINCVAVTLGQVVAYAIGASFQNVHNGWRWIVGLGAMPSFVQLAAIGFLPESPRILLLRSDVAGARAITAKIYPLATIEQVDRKIEIMKAAVDQSIEYNANSTWFERLKSLVMVGTNRRALIIGCGLQAAQQLCGFNTLMYYSATIFAMLGFNNATAVGLIVATVNVLFTLVALKIVDPVGRRRTMLFTLPIMILALVFAAIFFYYLTLSTNGILIEDHDYPRSLSILVLLSMLLYVAGYATGLGNIPWQQGELFRLEVRGIGTSICTAVNWSCNMLIAGTFLSLMDAATPSGAFGIYAGFCVIGWVFCWMLYPETKNRTYEEITGE"
  },
  {
    "reference": "MGLSPISEAIEDIKAGRFVIIVDDESRENEGDLAMAAERVTPEAINFMAKYARGLICVPLTGERLDELKIPLMVQDNTSKYSTAFTVSVEAKHKTSTGISAPDRAATIKALVDPATRPEDLARPGHMFPLRARAGGVLVRAGHTEAIVDLARLAGLQPAGVICEIMSDDGTMARMPQLEALADRFGLKIISVADLIAYRRRTEKLVQRAVETMLPTRYGEFTVIAYKSITDPDEHLALILGDIATPEPVLVRVHSECLTGDVLGSLRCDCGEQVSLAMQRISQEGRGVFVYMRQEGRGIGFHNKLKAYALQDQGMDTVQANETLGFPADLRDYGVGAQILADLGLKDIRLLTNNPKKVVGLEGYGLRVVETVPIQAKPNPYNIRYLETKQKKLGHLLGIDSDSDAP",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The presence of GTP cyclohydrolase II domains is critical for the interaction of the designed protein with its intended targets.2. The Proton acceptor; for GTP cyclohydrolase activity active site of the designed protein must be optimized for substrate binding and specificity.3. For general function, the protein need meet that Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.4. The protein requires Zn(2+) to activate a critical catalytic site.5. A protein with manganese ion binding, magnesium ion binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity functions is required to enable riboflavin biosynthetic process.6. Ideal protein should have a binding affinity for key enzymes in the Cofactor biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "ERVSDAELPTKAGSFRAIAYRDAASGLEHIALVKGNLADTESPLVRIHSECVTGEALGSLRCDCGDQLQQALSLIEAEGTGCIVYLRGQEGRGIGLGNKIAAYGLQDGGLDTYDANRALGLPADDRDYSVAAAILSDLRVKQLRLLTNNPKKVAGLERCGITVHSRRL",
    "response#2": "QVVEVDMPTDLGDFRLAAYEEVLTGDVHLALSKGGPFSPDEPVLVRVHSQCVTGDIFGSARCDCGDQLAAAMAQIEREGKGVVLYMKQEGRGIGLLNKLRAYKLQEEGMDTVEANEALGFKMDHRDYGIGCQILRDLGLRQLRLMTNNPRKRIALSGYGLEIVERVPFYDELLDRSVCHFPSQFGDFQLYAFRNTLDGSEHIAIVKGDPAQFERQPVMVRMHSECLTGDALGSLRCDCRMQLQAALKTIENAGLGVVVYLRQEGRGIGLVNKLKAYSLQDMGLDTVEANERLGFPADLRDYGMGAQILNDLGIRQIRLITNNPRKIAGLKGYGLEIVDRVPLFSQVLGSARAAVVDMPTSYGHFTVYGYQELYSDKEHVVYMKPYEGIPDIRLHSECLTGDVFHSKRCDCGEQLENGLRHIDEHGGLLIYLRQEGRGIGLINKLKAYEKIEQGLDTVEANEALGFDADLRDYGVAAAILRDLDIPSVNLITNNPRKIDGLKKYGIDVRLRIPLFREVIGG",
    "response#3": "RAAVVDMPTSYGHFTVYGYQELYSDKEHVVYMKPYEGIPDIRLHSECLTGDVFHSKRCDCGEQLENGLRHIDEHGGLLIYLRQEGRGIGLINKLKAYEKIEQGLDTVEANEALGFDADLRDYGVAAAILRDLDIPSVNLITNNPRKIDGLKKYGIDVRLRISSAIPTQLGDFRAHAYRSTFDGTEHLALVMGDVASAGQSEQGVLVRVHSECLTGDVMGSLRCDCGTQLAQSQADIANEGCGVLVYLRGHEGRGIGLVNKIRAYGLQDKGLDTVDANTAQGLPADARTYDVAAQILTDLGVRRIRLISNNLEKYRSLNNSAVHVVERRTVALDGGRAAVVDMPTSYGHFTVYGYQELYSDKEHVVYMKPYEGIPDIRLHSECLTGDVFHSKRCDCGEQLENGLRHIDEHGGLLIYLRQEGRGIGLINKLKAYEKIEQGLDTVEANEALGFDADLRDYGVAAAILRDLDIPSVNLITNNPRKIDGLKKYGIDVRLRIYGAPGF"
  },
  {
    "reference": "MAVRLLVASRNAKKLAELQRVLDAEGISGIEPVSLADVPDFPEAPEDGATFVENALIKARSGARATGLACLADDSGLTVDALNGMPGVLSARWSGRHGDDAANNELLLAQLSDVPDERRGAAFVSACALVGPGVPGDEVVEEGRWEGAVLHGPRGDGGFGYDPLFLPAGQPAGAERAAAQLTPEEKDAESHRGRALRALVPALRELAAGA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must possess XTP diphosphatase activity, ribonucleoside triphosphate phosphatase activity, ITP diphosphatase activity, dITP diphosphatase activity, nucleotide binding to support nucleotide metabolic process, purine nucleoside triphosphate catabolic process under certain conditions.2. The protein must exhibit the following characteristics: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP; Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions..3. The designed protein should have a binding site that is specific to the target Mg(2+), substrate ligand.4. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.5. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for dITP + H2O = dIMP + diphosphate + H(+). The designed protein sequence is ",
    "response#1": "MDLVFATNNHGKLREISHILGDRFRCITLSDIGCREEIVEDGDTFEENARKKAAAAFAVTGKMVLADDSGLSVAALDGAPGVYSARYAGEGTSYADNNAKLLRELEGAPVEKRTAYFVSAICLVFPDGSERTVEGEVRGRILTEPRGTNGFGYDPLFIPDGYDKSFAELGDEVKTRISHRARALERMADLLETVLEEE",
    "response#2": "MKLVFATNNQHKLSELTAILGEQYELISLADIGCHTEIPETAATLEGNALQKAQYVFDHYQLDCFGDDSGLEVDALNGAPGVYSARYAGEQRNADDNMNKLLHALQGQTNRSARFRTVIALNLNGKQHLFEGICEGRITHALQGTNGFGYDPVFVPDGYDKTFAEMSLDEKQSISHRGRAVQKLVAFLNT",
    "response#3": "MNVLILAGSTGRRFGSTTPKQFMRIAGVPLFWHSARAFLMHRRVERIILVVPAEYRNAYAELLGGYSLELPVTVVEGGATRQQSVFLGLQAVPPECKVAVIHDAVRPFVDSDVIDGIIAALEKHQGAIPALPVADTLRRGDEAGLVEATLDRRELRAVQTPQAFHFDVILAAHRAAAEQGLAGATDDASLVERQGIPVVIVPGEPRNMKITRPEDLELAEALLSR"
  },
  {
    "reference": "MVAEARRCLAVVLAAGEGTRMRSATPKVLHRLAGRSMLAHVLAAVAEASAEAVVVVVGPGRDDVAAEARRAFPGAEVAVQAERLGTAHAVLAARDAIARGYDDVLVLFADVPLIAPGTLAAMRDGLAEGAGLVALGFSAADPTGYGRLLTRDGALVAIREHKDASAEERAVTLCFAGPMAFDGATALDLLGRIGCDNAQGEFYLTEAVELTRADGRRCLALSCPERDAMGVNDRVQLAAAEAAFQARLRLAAMRAGVTLQAPDTVFLSHDTAFGRDVTVEPHVVFGPGVSVAEGAVIHAFSHLEGARVGPQANVGPHARLRPGAVLGQGAKVGNFVEVKSADIGAGAKVSHLSYIGDASIGADANIGAGTITCNYDGYGKFHTAIGAGAFIGSNSALVAPVSIGRNAYVASGSTVTEDVPEDALAIGRARQTTKAGRGAALRAARAAAKAARQG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein contains novel MobA-like NTP transferase domains that confer a unique function or activity.2. Ideal protein should have a binding affinity for key enzymes in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway to enhance the overall efficiency of the pathway.3. Mg(2+) is required for the proper folding and stability of the protein.4. A protein that can perform UDP-N-acetylglucosamine diphosphorylase activity, glucosamine-1-phosphate N-acetyltransferase activity, magnesium ion binding in cytoplasm to support cell wall organization, regulation of cell shape, peptidoglycan biosynthetic process, lipid A biosynthetic process, UDP-N-acetylglucosamine biosynthetic process.5. The acetyl-CoA, UDP-N-acetyl-alpha-D-glucosamine, Mg(2+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "LVLAAGHGSRMKSRVPKVLHHVAGQTMIDWVLDAVAPLTPNRPITVIGVGAESVQTHVGERSDFVLQAQQLGTGHAVQQAQDKLSASHGVTLIMSGDTPMFRAETLSEFIAAHRQSNNAVTVLTAVTQEPTGYGRIVRADDDTVLKIVEQKDASVTERRIQEINTGVYVFDNQLLFEALAQVKNNNAQGEYYLPDTLDILRQAGHQIGASWKTDDPAEVMGINDRVALAQAEKTMRRRINEGHMRNGVTVMDPTRLDIRGTVQTGRDVSIDVNVILEGEITLGDRVKIGPHTVIRHAKIANDVEILSHCVIENVVIGAGCRIGPFARLRPDTVLAEQVHVGNFVELKKSTVANGSKINHLSYVGDSKVGSGVNIGAGTITCNYDGANKHKTIIEDRAFIGSNTQLVAPVKVGAGATIGAGSTITINAPPDALTYTRSPQEAKPGWADRFHAMKKKK",
    "response#2": "VIVLAAGAGTRMKSATPKVMHQIGGRSLLHHAVSAAAGVAPEHLVVVLRHERELVADHLDQVTAAVEQSIIVADQDSVPGTGRAAECALTQLPQDLRGTVVVTYGDVPLLTSQTIADLVRFHEENSHAVTVLSAHVDNPHGYGRIIRDAAGALEKIVEEKDADDAQRKITEINSGIYAFDAGVLRSSLEQVSTDNAQGEKYLTDVIGLARDAGSSVAAMVTDDLWDVDGVNDRIQQAEAAYQRRLRAAAMENGATLVAPETVFFSHDTRLGRDVVVEPNVVFGPGVTVGDHAVIHAFSHLAGADVGEGVSIGPFARLRPGARMAAGSRAGNFVEVKNAEIGEGAKLNHLTYVGDATVGPRANLGAGTITCNYDGFSKHRTEIGADAFVGVNAALVAPVRVGEGAYVGTGSVVTEDVPDGALAIARARQVNKEDYVARERAWQDRRRAEAMADGATLIAPETVWFAYDTKVGRDVTIEPNVVFGPGVTIADGAIIHAFSHLEGASVGAGCSVGPYARLRPGAVMEAKSRVGNFVEMKKAVLGEGAKANHLTYLGDAEIGAGANIGAGTITCNYDGFFKYKTKIGAGAFIGSNSALVAPVTIGDGAIVGAGSVVTREVSADALALVRPARADLAEAEAAFQARMRAQMMADGVTLIAPETVFFAHDTKIERDVVIEPNVVFGPGVVIEEDVVIHAHSHVEGAHLKRGAHAGPFARLRPGAELGEGSKVGNFVEIKKSQLAEGAKVSHLTYIGDASIGANANIGAGTITCNYDGYDKHRTVIGENAFIGSNTCLVAPVTVGAGAFTATGTIVTEDVPADALALARTP",
    "response#3": "MTAVILAAGQGTRMKSKLYKVLHPVCGKPMVSHVVDNVRGVGADRVVTIVGFGAEKVEEEVGGRCEFVLQEKQLGTGHAVLQAEQNLGGLDGTTIVVCGDTPLITPETMKALIDHHNQTKAKATILTAQLANPDGYGRIIRTQDNEVMAIVEQKDASEEQLSIREVNTGIMAMNAKHLKFWLSQVKNDNAQGEYYLTDIIAMAVQDGVKIVTAQPAQEWEVAGVNSKKQLSKLERYYQAEIANKLLEAGVELLDPDHTYVDAGVIIGQDTVIEGGVTILGQTTIGQDNLLTYGTRIVDSVIGNHNTITDSHIESATIADHVTIGPFAHLRPEADLANGVHIGNFVEVKQASLAANTKAGHLSYIGNATVGQSVNIGAGTIFVNYDGVHKFNTNVGDHAFIGSNTKLVAPVTIANETITAAGSTITEDIPEHAMGIARSRQRNIENWKRPKK"
  },
  {
    "reference": "MKIVLGVTGGIAVYKAIDLTSKLVQNGHEVRVIMTESAEQFVTPLAFQALSRNPVYTDIFVEQNPAEIQHIALGDWADIMIIAPLTASTLGKIAHGISDNMLTATVLAFTKTIYLAPAMNSNMYNNPAVTDNIQTLLKRGFKFIEPGEGFLACGYIAKGRMSEPLDIMKFIGEDNIEKDLQGKKVLVTAGPTIETIDPVRYLTNRSSGKMGYSLAEAASLRGADVTLVSTDVGIPVPNGVRHIKTESAQDMFNAVKDNMEQDIIIKAAAVADYTPVETHDQKLKKQEGDLTITFKRTEDILKYIGEHKTTQFVVGFAAETEHVEMYARGKLIRKNADVIVANNVGDDSIGFKSNDNAVTIYFKDGTHIDIPKQSKLNIAHEILSHVKEKGFK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The CTP binding site should be stable and able to withstand changes in environmental conditions.2. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.3. A protein that can modulate the overall flux of metabolites through the coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway, enhancing or suppressing its activity as needed.4. The Proton donor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.5. The designed protein must possess phosphopantothenoylcysteine decarboxylase activity to support pantothenate catabolic process under certain conditions. The designed protein sequence is ",
    "response#1": "MNIVLGVSGGIAAYKACILLRLLKESGHDVTVIPTEAALKFVGEATWEALSGHQVASGVFSDVSEVKHVRLGRHADLVVVAPATADLLARAAHGRADDLLTSTLLTATCPVVFAPAMHTEMWEHAATRANVATLRERGAHVVEPDSGRLTGADTGPGRLPEPESLAAAIRRRLDGG",
    "response#2": "MNIVLGVSGGIAAYKACILLRLLKESGHDVTVIPTEAALKFVGEATWEALSGHQVASGVFSDVSEVKHVRLGRHADLVVVAPATADLLARAAHGRADDLLTSTLLTATCPVVFAPAMHTEMWEHAATRANVATLRERGAHVVEPDSGRLTGADTGPGRLPEPESLLRAAARALAGV",
    "response#3": "MNIVLGVSGGIAAYKACILLRLLKESGHDVTVIPTEAALKFVGEATWEALSGHQVASGVFSDVSEVKHVRLGRHADLVVVAPATADLLARAAHGRADDLLTSTLLTATCPVVFAPAMHTEMWEHAATRANVATLRERGAHVVEPDSGRLTGADTGPGRLPEPESLLDLIEHHFQPGLLKDKKIVITAGPTIEPLDPVRYMTNFSSGKMGYALAEEAEKRGGKVVLITGPVNLTPPYGVGIIKINTTIEMLNAVEKNFDDCDILIKAAAPLDYRPETVSKDKIKKNGESLQLKFVPNPDIAAYFGKLKKNQIIVGFAAETNNVIEYAKEKLKKKNFDFIVVNDISKDGAGFRTDTNIVSIIHRDGTVTELPKMSKREVAHAILDRVLG"
  },
  {
    "reference": "MSEVLDLSAEARERAGKGASRAIRREGRVPAVIYGEKQEPASIHVEEKVLMKLLHTGHFFNSVVMLDVGGKKVRTLPKDVQFHPVTDRPLHVDFLRIGEHTKVNVNVPVHFKDEELSPGLKRGGVLNVVIHDLGLSVDAAEIPEEIVISLKGLEVGESIHLSQVALPKGASAQDHEDITIATIVAPSALKSAEGEEAAEAGEEAAEG",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance..2. A protein that can perform structural constituent of ribosome in ribonucleoprotein complex to support translation. The designed protein sequence is ",
    "response#1": "MFRNQ",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTE",
    "response#3": "MTLPIGCKVTLRGEAAEGFLETSLGIVEKRLNESQFDIFGNVSFGVEEHTDYPGMRYDPNIGIFGMDITVVVNRPGYRVSKRRIAKRKIPTSHKITKEDTIL"
  },
  {
    "reference": "MAKTNLNDFDKIIVLDFGSQYNQLITRRIRDFGIYSELLPHDLSIEKIKEMAPKGIIFSGGPNSVYDKGALKVDPEIFKLGIPILGICYGMQLMSYDLGGKVEKADNSEYGRADIEVIDPNAVLFEGLPREQYVWMSHGDLVTKAPEGFTITAKSKNCPISAIANDEKKFYGIQFHAEVRNSEYGLDILKNFAFKVCGAKANWTMDDFIEMQVDEIRKKVGDKKVILGLSGGVDSSVTATLLHKAIGDQLTAIFVDHGMLRKDEGDQVMQALNKDLGVNIIRVNAQERFLNKLKGVTDPEQKRKIIGKEFIEVFNEEAKKLKDVDFLAQGTLYTDVIESGTNTAQTIKSHHNVGGLPEDMHFELIEPLRKLFKDEVRELGEKLGIPHDLVWRQPFPGPGLGIRVIGEVTEDKLKIVRESDAILREEIKNAGLQEDIWQYFTVLPGIRSVGVMGDGRTYDYTIGIRAVTSIDGMTADFAQIPWDVLSKISTRIVDECDHINRVVYDITSKPPSTIEWE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein contains novel Glutamine amidotransferase type-1, GMPS ATP-PPase domains that confer a unique function or activity.2. A protein with ATP binding is needed to enhance glutamine metabolic process in a specific context.3. A protein should be able to manipulate the activity of enzymes in the Purine metabolism, GMP biosynthesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "IVGDQIAQIRGQVGDRRVICGLSGGVDSAVAAALVQRAVGDQLTCVFVDHGLLRQGEAEQVKNDFVAATGADLVVADESQRFLDALAGVTDPETKRKIIGREFIRTFEDVAKRLNADQPIDFLVQGTLYPDVVESGGGEGAANIKSHHNVGGLPDDFQFSLVEPLRTLFKDEVRAVGLELGLPEDIVWRQPFPGPGLAIRIIGEVTAERLEVLRTADAITREEMGAAGLDRKVWQCPVVLLSDVHSVGVQGDGRTYGSPIVLRPVTSEDAMTADWARIPYDVLEKISTRITNACPQINRVVLDITSKPPATIEWT",
    "response#2": "VLDYGSQYNQLISRRIREIGVFSELKSHKISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGGKVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSADCPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALG",
    "response#3": "ILTLDFGSQYTHLITRRLREIGVYSEMLPCTQKLADLPFKPKGIILSGGPYSVYEDGAPHADPAVFELGVPVLGICYGLQEIAYRLGKDNVVAGTAREYGHADLNAQRLDNQGHVDKLFAGLEEHVKVWMSHGDKLVKLPEGFHTIATTANSEYAGIAHETKPVYGIQFHPEVTHTPDGAKLLRNFIG"
  },
  {
    "reference": "MIDKIKQHIEEAKAFNEKNKESLEQFRIKYLGSKGLLKELFNEFKNIPNDQKKDFGQVINTLKAVAEEKVKQIQEELESKEESKGVFGDLTRAAEPVIIGSRHPISIVKNQIIDIFANIGFNVSEGPEIEDDWHNFTALNLPEYHPARDMQDTFFIQTNPDVLLRTHTSSVQVRYMENHKPPIRTISPGRVFRNEAISSRSHCIFHQVEGLYIDKDVSFADLKQTLLYFTKEMFGKSKIRLRPSYFPFTEPSAEIDIYWGLKTETDYRITKGTGWLEIGGCGMVDPNVLKNCDINPDEYNGFAFGMGVERIAMLLYQIGDIRMFYENDVRFLEQFKANI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must be able to perform phenylalanine-tRNA ligase activity under specific conditions. The designed protein sequence is ",
    "response#1": "DRVPDLLAGYLAFVAIFCAVTAIVPPLRAPLEWLRIAIEVVSVEAPPSLAIAVFLGILASAVRRRMRVAWWFVVVYVVLGRVLGGVEFTIDTEAADPVTLQIGATGTWLRLVFGVAWLAILLVARRRFSARTQHHSGWRALGTYLALVVAGTLLGWGLLALFPGTLSNAADRLGWAVTYVLGGLGDPGAVGIDGAAPRAVTFLCGLFGALAVLAAAYVLY",
    "response#2": "DHRKLGKKLDLFHVQEEAPGMVFWHPKGWRLYQAVEQYMRQKQLDNNYQEVKTPQIVDRVLWEKSGHWGKYHENMFTTHSENRDYAVKPMNCPCHIQVYNQGLKSYRDLPFRMAEFGSCHRNEPSGALHGIMRVRNFVQDDGHIFVTEGQIQQEVSEFIDLLHEVYADFGFDNIVYRLSTRPEQRVGSDEVWDKAEKALSEALDSAGLDWEELPGEGAFYGPKIEFSLKDAIGRVWQCGTIQVDFSMPTRLGAQYVSEDGSRQTPVMLHRAIVGSLERFIGILIEDTEGAFR",
    "response#3": "DRWIVSRLNAAAETANRAIEQYRYHELAQELWKFFWHEFCDWYLELKKVSATGWGNAVAAFETALRLLHPAMPFLTEELWQRLERKEGDPKSIALAQYPQYRAELADSEAEKEVAIIQEIVTLARTLRTESKLDPKQQLD"
  },
  {
    "reference": "MMPVSRLAGALIPAMTFLSYLRVESWEPCVEVIPNISYRCMEVNLYKIPDNIPSSAKNLDLSFNPLRHLGSHSFSNFSELQVLDLSRCEIQIIEDGAYWGLNHLFTLILTGNPIQHLALGTFFGLSGLQKLVAVEINLTSLEDFPIGHLKTLKELNVAHNSIHSFKLPEYFSNLSNLEHLDLSNNQIRNIFRKDLQILHQIPLLNLSLDLSLNMIDFIQPGAFKEIRLHKLTLRSNFDNMTVMKMCIQGLAGLEVHQLVMGEFKNERNLDRFDKSALEGLCNLTIEEFRMAYVSDFSSHDIDVFNCLANVSAVSLVSLNLNELERFPEDYRWQSLELVNCKLQPFPALALSSLKRFVLTANKNSNTIVEFKLPSLEFLDLSGNVLSLRGCCSQSDFGTTKLKYLDLSFNNVIVMNSNFLGLEQLKHLDFRYSTLKHVNDFPPFLSLTKLLYLDISHTHIHVVFHGIFLGLYSLKVLKMAGNSFQDNFLPDIFTDLTDLIFLDLSYCQLEQVSRGAFDSLLRLQVINMSSNNLLSLDTLPFKYLHSLQVLDCSFNHIVASKVQELQHLPSNLTLLNLTQNDFACICEHQSFLQWVKDQRQLLVEVEQMVCATPSDMHGRPVLSFRNTTCQMSKTVISASVLSVFIVSLMLVLAYKFYFHLMLLAGCRRYGGGECTYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIQEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSHAGII",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The designed protein have TIR domains with specific sequence motifs that are crucial for its activity.3. Include a signal peptide as an essential component of the protein.4. The designed protein should be involved in positive regulation of oxidative stress-induced neuron death, positive regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway, positive regulation of NLRP3 inflammasome complex assembly, toll-like receptor 4 signaling pathway, positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway by localizing to Golgi apparatus, phagocytic cup, lipopolysaccharide receptor complex, external side of plasma membrane, perinuclear region of cytoplasm and exhibiting signaling receptor binding, identical protein binding, lipopolysaccharide binding, protein heterodimerization activity, lipopolysaccharide immune receptor activity. The designed protein sequence is ",
    "response#1": "MANPDPSLHGALNLLRAVERDRLVHLKDKLGSLNPGSQESKLLHAMVLLVLGQNTEARTSLESLKADTVAQLIACQWIGVDSTEGPEEPPDLSWSVARLYHLLAEENLCPASTRDMAYQVALHDLASQGNHLLGQLQKEARDRCSSDECFDAFICYCPSDIEFVHEMIRQLEQTNYRLKLCVSDRDVLPGTCVWSIASELIEKRCRRMVVVVSDEYLQSKECDFQTKFALSLSPGAHQKRLIPIKYKPMKKEFPSILRFITVCDYTNPCTQNWFWTRLAKARR",
    "response#2": "ICYDAFVSYSERDSYWVENLMVQELEHFRPPFKLCLHKRDFIPGKWIIDNIIDSIEKSHKTIFVLSENFVKSEWCKYELDFSHFRLFDENDDAAILILLEPIEKKAIPQRFCKLRKIMNTKTYLEWPAG",
    "response#3": "MANPDPSLHGALNLLRAVERDRLVHLKDKLGSLNPGSQESKLLHAMVLLVLGQNTEARTSLESLKADTVAQLIACQWIGVDSTEGPEEPPDLSWSVARLYHLLAEENLCPASTRDMAYQVALHDLASQGNHLLGQLQKEARDRCSSDS"
  },
  {
    "reference": "VIGGAECDINEHPSLALIYSTSMRFHCAGTLLNQEWVSFTMWDKDIMLIRTLCAGVLEGGKDTCLAHPCAQPLLPAFYTKVFDYIPWIK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in regulation of blood pressure, potentially with improved properties such as stability, specificity or selectivity.2. The designed protein have Peptidase S1 domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "VVGGDECNINEHRFLVAIFNSNGFVCSGTLINQEWVLTAAHCDSTDFQIKLGAHSKKVLNEDEQIRNPKEKFICPNKKNDEVLDKDIMLIKLDSRVSNSEHIAPLSLPSSPPSVGSVCHIMGWGSITPIEVTFPDVPHCAYINLLDDAACQPGYPEVLPEYRTLCAGILEGGKDTCNYDSGGPLICNGQFQGIVSYGAHPCGQSLKPGIYTKVFDYNDWIQSC",
    "response#2": "VVGGDECNINEHRFLVAIFNSNGFVCSGTLINQEWVLTAAHCDSTDFQIKLGAHSKKVLNEDEQIRNPKEKFICPNKKNDEVLDKDIMLIKLDSRVSNSEHIAPLSLPSSPPSVGSVCHIMGWGSITPIEVTFPDVPHCAYINLLDDAACQPGYPEVLPEYRTLCAGILEGGKDTCNYDSGGPLICNGQFQGIVSYGAHPCGQSLKPGIYTKVFDYNDWIAANT",
    "response#3": "VVGGDECNINEHRFLVAIFNSNGFVCSGTLINQEWVLTAAHCDSTDFQIKLGAHSKKVLNEDEQIRNPKEKFICPNKKNDEVLDKDIMLIKLDSRVSNSEHIAPLSLPSSPPSVGSVCHIMGWGSITPIEVTFPDVPHCAYINLLDDAACQPGYPEVLPEYRTLCAGILEGGKDTCNYDSGGPLICNGQFQGIVSYGAHPCGQSLKPGIYTKVFDYNDWIQSC"
  },
  {
    "reference": "MNFENNLAYAKALDSNDPLKELRNEYLFPKQNGKPFIYLCGNSLGLQPKVAKEVLQKQLDNWENLAVEGWFEGESPWMFYHKELKKLMAPIVGAKPAEVCPMNTLTVNLHLLMVSFYKPTAKKFKIIMESGAFPSDQYAIESQVRFHGFDPADAIIEVAPREGECTLRTEDIVKSIEENCDEIALVLFGGVNYFTGQWFDMQAIAKAGHKIGAVVGFDLAHAAGNVPVQLHDWDIDFACWCSYKYQNAGPGGISGIFVHEKHFSDSSLNRFAGWWGYKEKERFKMEKGFIPELGADGWQVSCTQVMPMALYHASLQLFEKAGFITALRNKSKTLTSYLFYVIDQINISLGGEQYKIITPRTEDDRGAQVSIIAKENGKQVFDALVAENVLGDWREPNVIRLSPVPMYNSFEDVFKTGELLLKLSKVSK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively.2. The designed protein must have at least one Aminotransferase class V domain.3. A protein that can perform 3-hydroxykynureninase activity in cytoplasm to support tryptophan catabolic process, quinolinate biosynthetic process, 'de novo' NAD biosynthetic process from tryptophan, anthranilate metabolic process, L-kynurenine catabolic process.4. The protein design should take into account the physiological conditions of the Cofactor biosynthesis, NAD(+) biosynthesis pathway.5. The protein should be designed to have high stability and activity towards H2O + L-kynurenine = anthranilate + H(+) + L-alanine.6. The designed protein should have a binding site that is specific to the target pyridoxal 5'-phosphate ligand. The designed protein sequence is ",
    "response#1": "ELMAPLVGAKESEVALMGTLTGNLHLLMASFYKPTKERYKIIFEGKAFPSDHFAIESQALHHGLDPEDAMVLIEPVSEDYPVLETSQILSVIDAHADSAALILLPGIQYYTGQFFDMKTITAHAHSKGLMVGWDCAHAVGNVELQFHDWDVDFAAWCSYKYLNDQXGXVNXMSXSLSEGVADLVGAGKNEVVAMNTLTANLHFMMVSFYQPTKTRFKILMEDHAFPSDHYAVESQVKFHGFNPEDAVLFIKPRDGEEAIRDEDVLKLIEDEGESIALIMLPGVQYYTGQVFDMKSITQAGHNKGCKVGFDLAHATGNIVMSLHEWQVDFACWCTYKYLNSGPXGXGHXGXXXGIXGXGXQXFXLSEGVADLVGAGKNEVVAMNTLTANLHFMMVSFYQPTKTRFKILMEDHAFPSDHYAVESQVKFHGFNPEDAVLFIKPRDGEEAIRDEDVLKLIEDEGESIALIMLPGVQYYTGQVFDMKSITQAGHNKGCKVGFDLAHATGNIVMSLHEWQVDFACWCTYKYLNSGPHXXSRXRXRXLPXFNWNXKQHWXLSEGVADLVGAGKNEVVAMNTLTANLHFMMVSFYQPTKTRFKILMEDHAFPSDHYAVESQVKFHGFNPEDAVLFIKPRDGEEAIRDEDVLKLIEDEGESIALIMLPGVQYYTGQVFDMKSITQAGHNKGCKVGFDLAHATGNIVMSLHEWQVDFACWCTYKYLNSGP",
    "response#2": "MAAAKLIGAKEDEVAHTSTLTSNIHNLFTSFYRPTEKRWKIVIEKGSFPSDWYAIHSHPKLHDKVLSQTQIDDAIIGLEPREGEDVLRTEDILKVIEENKDTIAVVWLPLVQYYTGQLFDIAAISPKVHDIGALLGIDMAHGTGNVECKLDEWNVDFAVWCTYKYLNAGPAAIGGLFVREGLDDGGRRLAGWWGNDAATRFQMNPEFRATPGAKGYQHSCTPVFSSIPLLATLNLIDKVGFANMVGKGKKLTGTLEKLLKASPYYAGQDASLPSFLSEGVADLVGAGKNEVVAMNTLTANLHFMMVSFYQPTKTRFKILMEDHAFPSDHYAVESQVKFHGFNPEDAVLFIKPRDGEEAIRDEDVLKLIEDEGESIALIMLPGVQYYTGQVFDMKSITQAGHNKGCKVGFDLAHATGNIVMSLHEWQVDFACWCTYKYLNSGPQ",
    "response#3": "MAKLIGAKEDEVAHTSTLTSNIHNLFTSFYRPTEKRWKIVIEKGSFPSDWYAIHSHPKLHDKVLSQTQIDDAIIGLEPREGEDVLRTEDILKVIEENKDTIAVVWLPLVQYYTGQLFDIAAISPKVHDIGALLGIDMAHGTGNVECKLDEWNVDFAVWCTYKYLNAGPAAIGGLFVREGLDDGGRRLAGWWGNDAATRFQMNPEFRATPGAKGYQHSCTPVFSSIPLLATLNLIDKVGFANMVGKGKKLTGTLEKLLKASPYYAELPLLEPELANLLSEGVADLVGAGKNEVVAMNTLTANLHFMMVSFYQPTKTRFKILMEDHAFPSDHYAVESQVKFHGFNPEDAVLFIKPRDGEEAIRDEDVLKLIEDEGESIALIMLPGVQYYTGQVFDMKSITQAGHNKGCKVGFDLAHATGNIVMSLHEWQVDFACWCTYKYLNSGPETHFNVYSTNLSEGVADLVGAGKNEVVAMNTLTANLHFMMVSFYQPTKTRFKILMEDHAFPSDHYAVESQVKFHGFNPEDAVLFIKPRDGEEAIRDEDVLKLIEDEGESIALIMLPGVQYYTGQVFDMKSITQAGHNKGCKVGFDLAHATGNIVMSLHEWQVDFACWCTYKYLNSGPETHFLVGDANLSEGVADLVGAGKNEVVAMNTLTANLHFMMVSFYQPTKTRFKILMEDHAFPSDHYAVESQVKFHGFNPEDAVLFIKPRDGEEAIRDEDVLKLIEDEGESIALIMLPGVQYYTGQVFDMKSITQAGHNKGCKVGFDLAHATGNIVMSLHEWQVDFACWCTYKYLNSGP"
  },
  {
    "reference": "MQQYLQNKKGLVQNVFDKVYDKYDLMNDFMSLGIHRIWKKNFINMINPCSNKKLIDVACGTGDIGKLYLDYTDINSQVTCVDPNQGMINQGKKKLANYKNIEWILAPAENLPIPENTFDFYTISFGLRNAKSLERSLSEALRVLKPGGRFLCLEFSKIQNSNLDFIYKNYSKLIPLVGKIVVGEKKPYEYLVKSIENFLNQEELISLMKEKNFKKCTYRNLSGGIVSIHSGWKI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The desired protein should be able to perform a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine2. We require a protein that can specifically bind to the metabolites produced in the Quinol/quinone metabolism pathway.3. A protein with 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity, S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity, demethylmenaquinone methyltransferase activity is needed to enhance menaquinone biosynthetic process, methylation, ubiquinone biosynthetic process in a specific context.4. The S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "MFSDKLIKKYDVMNDLMSGGLHRMPIQGGMTNDRIIESIKFALKHGINYFDTAPIYGFGLSEKRIGSALKNKREDFILSTKVGRILKKSKFSSKSRVWPKSFKKNKLITHDFSYEGIWNSLEQSLKRLDTDYIDLIYLHDVESHSFDEILESVFPALVKLKEKGIIKAIGVGCGEWRVIDQILKNSDIDCFLIAGRYTLLEQDSLNKILPLCEKRNVSIILGGPYNSGILAISEPNDSNYNYQAAPSDILKRAKRAENICKKYNVSLKAAALQFPLNHPFISSVIIGATTISQLENNVKLFDSTIPNDIWFELKSLKLISENAPL",
    "response#2": "MNDTNNTDQNASNKSENTTSFGYRDVPASEKTKKVAGVFHSVASKYDVMNDLMSMGIHRLWKKFTIELSGVRTGNRVLDIAGGTGDLAAKFSRLVGPTGQVVLADINESMLKVGRDRLTNEGVAGNIEYVQANAEALPFPDNHFDCVTIAFGLRNVTDKDAALRSMYRVLKPGGVALILEFSKPVLPGLSKLYDLYSFNILPLMGRLVANDADSYRYLAESIRMHPDQETLKGMMDDAGFARTTYHNMTGGIVALHRGIKP",
    "response#3": "MLNQHFSTLSGFGTLLKQHGIDFEHLQLDTSTASAETLLATQADNLNASEREHVLTVLKSHLDELRTLKGKLDEKHHVFYGLHPQMANLTGLIMDSKDPLHGDNVKQYLAKNDFFVTRAMNVFSTSPHVMEGYDFLNHLLSLRRDVYSAAGHQYGEELKAMTDADFAKMYAEENKWVRQKVLPMMKHYADFIISEQV"
  },
  {
    "reference": "MGQKVNPNGFRYGITKAHNSVWYADKMQFASHLLEDQKIYDFFDKKVRQLQIGNVQIKRNQNGLILIYVYTAKPAVMLGTNGENIKNLTKDLQKILKNKKANISIQVIELKKPDLNARLLAEDIAIKLENRGSFRLAQKFAIKAALKAGAKGIKTSVSGRLNGVDMARTEGYNEGEMKLHTLRQDVDYAATTAKTTYGILGVKVWVSLGEILSDEQKAKQEEMDLLNAPKDRRVRRGGERHASTKKN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The ideal protein would be one that plays a critical role in translation through mRNA binding, rRNA binding, structural constituent of ribosome within ribonucleoprotein complex, ribosome.2. The designed protein should contain one or more KH type-2 domains that are essential for its function.3. The protein must exhibit the following characteristics: Binds mRNA in the 70S ribosome, positioning it for translation.; Binds the lower part of the 30S subunit head. The designed protein sequence is ",
    "response#1": "IARQLEARVAFRRAQRQAMQRTMRAGAKGIKTQVAGRLNGADMSRIETHAQGTVPLHTLRADIDYSWDEAMTTYGKLGVKTWL",
    "response#2": "XISSQLERRVMFRRAIKRSVQNAMRQGAKGIKIEVSGRLGGVEIARREWYREGRVPLHTLRANIEYNTSEAHTTYGIIGVKVWR",
    "response#3": "SPIGCRVTLRGERMYDFLERLIFIALPRVRDFQGIPRRGFDGNGNYNLGIKEHTIFPEISFDKTDAVKGLNITIVTTADNDDMARTLLERVGLPA"
  },
  {
    "reference": "MSAFNFGSTGASTGGFTFGTAKTATTTPSMGFSFSTPSTGGFTFGTPSQPASSAPSTSLFSLTTQAPATQTPGFTFGTTPASTAAGFSLGISVPKLSLSNTTATPATANPGGFGLGGSTLTNAISSAVTSSQGMASTGFVFGSSTTSTAPATTSGGFSFTSGSTSQAGTSGFNIGSLGSAAQAQPTVLTGLAFTASTPATSAAGVSQPPAPTPTAAATSAGPTLFASIAAAPTSATTGLSLCAPATTTGPAGAGTLGFSLKAPGAASSTSTVTSTSTATTTATTTGFALNIKPLVPAAISSNAAGTAAPSGSSAATGTATSPVMTYAQLESLINKWSLELEDQERHFLQQATQVNAWDRTLIENGEKITSLHREVEKVKLDQKRLDQELDFILSQQKELEDLLSPLEESVKEQSGTIYLQHADEEREKTYKLAENIDAQLKRMAQDLKDIIEHLNTSGGPADTSDPLQQICKILNAHMDSLQWVDQNSALLQRRVEEVTRVCEGRRKEQERSYRITFD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein have Nucleoporin NSP1-like C-terminal domains with specific sequence motifs that are crucial for its activity.2. The designed protein should have signaling receptor complex adaptor activity, PTB domain binding, Hsp90 protein binding, SH2 domain binding, Hsp70 protein binding within mitotic spindle, Flemming body, cytoplasm, ribonucleoprotein complex, nuclear pore to regulate positive regulation of mitotic cytokinetic process, negative regulation of cell population proliferation, positive regulation of mitotic nuclear division, centriole assembly, mitotic centrosome separation.3. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "RPVIRLEGCDLRHPLKVAKGKSKNLPVSYGLSGGSREDSTYQIAWQFSPIDTLTESGDHGDVVTIGTYTAKNNRDRPTISAPGLYTLKSVSASQCEGEIQEPSSC",
    "response#2": "MESRYLSDRRSRFKSKGVFKADELRRQREEQQIEIRKQKREESLNKRRNLNAVLQNC",
    "response#3": "PVAPTMEVKSFPILDQCVGQVGLNFELSFTGAPPYYYNTKIYKLENGERKLYDAKRYTSEGTRNRFSYSPPKEGNYEIVFDTVSNKLFTEPIKLEPVKEYTFKTSMRL"
  },
  {
    "reference": "MATYLVGDLQGCYDELQLLLETVQFNPVQDKLYLVGDLVARGDKSLECLRFVKSLGAAAQMVLGNHDLHLISTALGIKKVSLQDHVEAIFTAPDFVELIDWLRHQPLLVHDEKCDFVMSHAGISPDWDLDTAKSCAREVENELQHGDYHYLISNMYDNQPDRWSADLQGLQRLRYSINVFTRMRFCYRDHRLDFACKASVEKAPQELIPWFNLDNPLFKVQNLVFGHWASLVDTPTPANIYALDTGCVWGNRMTMLRWEDKQYFVQGALKDYF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP..2. The designed protein must be optimized for bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity.3. The protein have enhanced H2O + P(1),P(4)-bis(5'-adenosyl) tetraphosphate = 2 ADP + 2   H(+). The designed protein sequence is ",
    "response#1": "MAVYAVGDLQGCLEPLQCLLEHVRFDPVQDRLWLVGDLVNRGPQSLQTLRYLYSIRESLVCVLGNHDLHLLAVARKNELLKKGDTLREILEAPDRDELLRWVRQQKLMHYDAERNIAMVHAG",
    "response#2": "DIIGDVHGCYDELMELIDKLGYEYKRGSYDHPDGRLLAFVGDLTDRGPHSLEVIRLVSHMVKKKTAYYVPGNHCNKLYRYMIGRKVQVKHGLETTVAELNALSEEERIEVIAQFKELYEQAPLYHSFPEDKLVITHAGIREDDIGSYGKRVETFVLYGDITGETNPDGTPVRRDWAATYQGDWWIVYGHTPVRRPRIIHRTVNIDTGCVFGGALTALRFPEIEFVSIPSRQR",
    "response#3": "MQFDIIGDVHGCYDELMELIDKLGYEYKRGSYDHPDGRLLAFVGDLTDRGPHSLEVIRLVSHMVKKKTAYYVPGNHCNKLYRYMIGRKVQVKHGLETTVAELNALSEEERIEVIAQFKELYEQAPLYHSFPEDKLVITHAGIREDDIGSYGKRVETFVLYGDITGETNPDGTPVRRDWAATYQGDWWIVYGHTPSELHIHDGVLLVNPGSVGQPRDGDPRAAYALFDTASRTMSLERVPYDIEATIAKIRAEKLPERLAERLG"
  },
  {
    "reference": "MSILKIHAREIFDSRGNPTVEVDLYTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALISKKVNVVEQEKIDRLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNAEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAASFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGTVPAGGVLGRDVQASALFFLSNKEFPLVSIEDPFDQDDWEAWQKFTASAGIQVVGDDLTVTNPKRISKAVGEKSCNXXXXXXXXSVTESLQACKLAQSNGWGVMVSHRSGETEDTFIADLVMGSWVSLCPLLPIKTGAPCRSERLAKYNQILRIEEELGSKAKFAGRNFRNPLAK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be expressed specifically in the plasma membrane The designed protein sequence is ",
    "response#1": "AFHDHILMPIITAITLLVLGLLIWIVIRYNAKANPVPAKFSHNTTVEIIWTIGPVLILMVIAIFSFRLLFAYNDMPKPYLTVKATGYQWYWGYEYPDQKISEITSVPLKQEEAVAKGVPYLLAADKPMVVPVNQVIRVQVTGADVIHAFALPAFGLKTDAIPGRLNETWFKADKIGVYYGQCSELCGVDHSNMPIEIHVVSQADFDAWA",
    "response#2": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPV",
    "response#3": "AFHDHILMPIITAITLLVLGLLIWIVIRYNAKANPVPAKFSHNTTVEIIWTIGPVLILMVIAIFSFRLLFAYNDMPKPYLTVKATGYQWYWGYEYPDQKISEITSVPLKQEEAVAKGVPYLLAADKPMVVPVNQVIRVQVTGADVIHAFALPAFGLKTDAIPGRLNETWFKADKIGVYYGQCSELCGVDHSNMPIEIHVVSQADFDAWR"
  },
  {
    "reference": "MSTTVRPDEVSAILRKHLAGFESEADVYDVGTVLQVGDGIARIYGLSKVAAGELLEFPDNVMGMALNLEEDNVGAVMFGKSTAVKEGDTVKRTGILASIPVGEAMLGRVINPLGEPIDGKGPIETSIRLPLERKAPGVIFRKSVNQPLQTGLKAIDAMIPIGRGQRELIIGDRQTGKTAVAIDTIINQKGKDVFCIYVAIGQKGSTIAQVVSTLEKYGAMEYTTVIASSASDPAPMQFIAPYAGAAIGEFFRDTGRHALVIYDDLSKQAVAYRQLSLLLRRPPGREAYPGDVFYLHSRLLERAAKITDDLETAKKMNDLPEPLKPMVKAGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLEPNLFNAGQRPAINVGISVSRVGGSAQIKAMKKITGTLRLDLAQFRELEAFSKFGSDLDKATKAQLDRGARLVEILKQDQYVPMAVEKQVAIIFAGTQGVLDQLDLQYIRRFEEEFLSLLEHKHSDILNSIAETGQMDVDVAKRLKEVAEQFMSTFKQKVTA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that The alpha chain is a regulatory subunit.; Produces ATP from ADP in the presence of a proton gradient across the membrane2. The desired protein should be able to perform ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate3. The ATP binding site should be located in a region of the protein that is accessible to the ligand.4. A protein that localizes to plasma membrane and can perform proton-transporting ATP synthase activity, rotational mechanism, ATP binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "GQRVIDAFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPELIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVSPPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYF",
    "response#2": "GIKAIDAMIPIGRGQRELIIGDRQTGKTAIAIDTIINQKGKGVYCIYVAIGQKTAAVARIVDKLREFGAMEYTTVVVASASDPAPIQYIAPYAGCAMGEYFMYNSKDALVVYDDLSKHAAAYRQLSLLLRRPPGREAYPGDIFYLHSRLLERAARLDDKMGGGSLTALPIVETQANDVSAYIPTNVISITDGQIYLEPGLFYSGFRPAINVGLSVTIKKLSGGIRTALAQYRELAAFAQFASDLDEATRKQLEHGQRVTELMKQKQYAPMSIAEMSLSLYAAERGFLQDVEIAKVGSFEQALISYFQREHAALLAKINEKGDFNDEIDAGIKAGIEKFKAMKKVAGTLRLDLASYRELEAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLPVEKQVLILYALTHGFLDSVSVDKILHFEQDLFDYFDGKHADLLETIRTTKDLPDTDALDAAITEFSEMINQKGLR",
    "response#3": "KGGKVGLFGGAGVGKTVNMMELIRNIAIEHSGYSVFAGVGERTREGNDFYYEMEESGVLDKVALVYGQMNEPPGNRLRVALTGLTMAEYFRDEGRDILFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLSQEMGQIQERITSTKTGSITSIQAVYVPADDLTDPAPATTFAHLDATVVLNRSIGSAAMKQVAGMLRIDLAQFRELETFAQFASELDPATRAQIVRGQHLQELLKQDQYSPLPVEEQVVVLYAGVRGYLDELPVESVRKFEKEFLDYMRTSKSSLLKLISEKKELTQEVEDELKKSIQEFLEGIMEVPVGEELIGRVVNPLGQPVDGQGPINTTKSRPIESPAFGVMARKSVHEPLQTGIKAIDALVPIGRGQRELIIGDRQVGKTSVAIDTILNQDGENMICIYVAIGQKESTVRGVVETLRKHGALDYTIVVTAAASQPAPLLFLAPFAGVSMAEEFMLQGKHVLIVYDDLSKQASAYRELSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNETYQNGSITALPFVETQAGDISAYIPTNVISITDGQIFLQSDLFNSGVRPAINAGLSVSRVGGAATPIADIKKLGGGIRLALAQYAELAAFAQFASDLDDATRAQLEHGQRVTELMKQKQYSPLSIAETAVSLFAAEKGFLNDVAINKVVDFEEALHAYMSNEQAALMATINEKGDYNKDIEASLKTALENFKSIKKLGGGIRLALAQYAELAAFAQFASDLDDATRAQLEHGQRVTELMKQKQYSPLSIAETAVSLFAAEKGFLNDVAINKVVDFEEALHAYMSNEQAALMATINEKGDYNKDIEASLKTALENFKSLELGQNA"
  },
  {
    "reference": "MYTDLTQRRVLLGVGGGIAAYKAPDLVRRLRERGADVRVVMTEAATAFITPLTLQAVSGRAVHQALLSAESEAAMGHIELARWAELVLIAPATADLMARLAHGHANDLLTTLCLASAAPLVLAPAMNRQMWAHPATQANAAALAARGVTLLGPAEGEQACGEVGAGRMLEPLALAEAIAARLAPAGPLGGRRVLITAGPTREPLDPVRYLTNRSSGRMGYAVARAAREAGAEVTLISGPVTLAAPAGVECLRVESAAEMAAAVRAQVAACDIFIGAAAVADYRAATPAEHKIKKQGETLTLELVRNPDILAEVAARDPRPFTVGFAAETDDLTGHAASKLRAKGLDMIAANRVGAGLGFDVAHNALLVLWPGGDRDLGEADKLTLARRLIELVGERYAAAVGAARP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that is capable of catalyzing reactions within the Cofactor biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "LRPYREDELRYLRGEDRQGPYQEHDRIYRYDVYNDLGEPDRDNPRPVLGGSQKHPYPRRGRTGRIPTKKDPNSESRLSLLEQIYVPSDERFAHLKMSDFAGYSIKAIVQGILPAIRTYVDLTPGEFDSFEDILKLYRGGLKLPSIPALEELRKSFPVQLIKDLLPVGGSYLLKFPKPDIIKENEVAWRTDEEFAREILAGLNPMVIRRLTEFPPKSTLDPSKYGDQTSTITPAHIEKNLEGLSVQQALDSNRLYILDHHDHFMPFLIDINSLDGIFTYATRTLLFLRDDDTLKPLAIELSLPHIEGNLTSAKSKVHTPASSGIESWVWQLAKAYVAVNDSGWHQLISHWLNTHAVMEPFVIATNRQLSVTHPVYKLLQPHYRDTMTINALARQTLINGGGIFEQTVFPGKHALAMSSAVYKNWNFTEQGLPDDLIKRGIAIKDPSSPSKVKLLIKDYPYATDGLAIWQAIEQWVTEYCAIYYPNDGVLQGDVELQAWWKEVREVGHGDLKDADWWPKMQSLPELTKACTTIIWIASALHAAVNFGQYPYAGYLPNRPTISRRPMPEPGSKEYTELDENPEKFFIRTITSQFQTILGR",
    "response#2": "LRPYREDELRYLRGEDRQGPYQEHDRIYRYDVYNDLGEPDRDNPRPVLGGSQKHPYPRRGRTGRIPTKKDPNSESRLSLLEQIYVPSDERFAHLKMSDFAGYSIKAIVQGILPAIRTYVDLTPGEFDSFEDILKLYRGGLKLPSIPALEELRKSFPVQLIKDLLPVGGSYLLKFPKPDIIKENEVAWRTDEEFAREILAGLNPMVIRRLTEFPPKSTLDPSKYGDQTSTITPAHIEKNLEGLSVQQALDSNRLYILDHHDHFMPFLIDINSLDGIFTYATRTLLFLRDDDTLKPLAIELSLPHIEGNLTSAKSKVHTPASSGIESWVWQLAKAYVAVNDSGWHQLISHWLNTHAVMEPFVIATNRQLSVTHPVYKLLQPHYRDTMTINALARQTLINGGGIFEQTVFPGKHALAMSSAVYKNWNFTEQGLPDDLIKRGIAIKDPSSPSKVKLLIKDYPYATDGLAIWQAIEQWVTEYCAIYYPNDGVLQGDVELQAWWKEVREVGHGDLKDADWWPKMQSLPELTKACTTIIWIASALHAAVNFGQYPYAGYLPNRPTISRRPMPEPGSKEYTELDENPEKFFIRTITSQFQTILGI",
    "response#3": "LRPYREDELRYLRGEDRQGPYQEHDRIYRYDVYNDLGEPDRDNPRPVLGGSQKHPYPRRGRTGRIPTKKDPNSESRLSLLEQIYVPSDERFAHLKMSDFAGYSIKAIVQGILPAIRTYVDLTPGEFDSFEDILKLYRGGLKLPSIPALEELRKSFPVQLIKDLLPVGGSYLLKFPKPDIIKENEVAWRTDEEFAREILAGLNPMVIRRLTEFPPKSTLDPSKYGDQTSTITPAHIEKNLEGLSVQQALDSNRLYILDHHDHFMPFLIDINSLDGIFTYATRTLLFLRDDDTLKPLAIELSLPHIEGNLTSAKSKVHTPASSGIESWVWQLAKAYVAVNDSGWHQLISHWLNTHAVMEPFVIATNRQLSVTHPVYKLLQPHYRDTMTINALARQTLINGGGIFEQTVFPGKHALAMSSAVYKNWNFTEQGLPDDLIKRGIAIKDPSSPSKVKLLIKDYPYATDGLAIWQAIEQWVTEYCAIYYPNDGVLQGDVELQAWWKEVREVGHGDLKDADWWPKMQSLPELTKACTTIIWIASALHAAVNFGQYPYAGYLPNRPTISRRPMPEPGSKEYTELDENPEKFFIRTITSQFQTILGR"
  },
  {
    "reference": "MLRRGYKASERRRHLSERLSWHQDQALSSSIYLLREMGPTGFLLREEEPEYMDFRVFLGNPHVCNCSTFPKGGELCKHICWVLLKKFKLPRNHESALQLGLGEREISDLLRGIHRVQTPQPGTNDENEHVEEDGYIKQKEIDSEDICSICQELLLEKKLPVTFCRFGCGNSIHIKCMKILANYQSTSNTSMLKCPLCRKEFAPLKLILEEFKNSSKLVAAAEKERLDKHLGIPCNNCKQFPIEGKCYKCTECIEYHLCQECFDSCCHLSHTFTFREKRNQKWRSLEKRADEVVKYIDTKNEIEEKMSHFQEKQGQVYTPKHIVRSLPLQLITKNSKLLAPGYQCLLCLKAFHLGQHTRLLPCTHKFHRKCIDNWLFHKCNSCPIDGQVIYNPLTWKNSAVNGQAHQSVSNRDIIHLSKQKEPDLFIPGTGLVLKQNRLGILPSIPQCNFDELNTPQSPKDAYENTTIDNLCSIKLDNSNSKKLTYDYKISQHFPRYLQDLPTVSFGKIPSQTLLPPIVHKNIVCPTAMESPCISGKFHTSLSRMTKGCKCNNHNLKKTPATKIREDNKRSTLLPEDFNLIVNWSTAKLSLSKRYSNCMGEITRKCSHLSRQPVSHSVNTKSTELSLIIEGVQL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding. The designed protein sequence is ",
    "response#1": "EMRLEKDKFSINLDVKHFSPEELKVKVSGDFIEIHGKHEERQDEHGYVSRDFQRRYKIPVDVDPLSITSSLSPDGVLTVCGPRL",
    "response#2": "RLPSWIESGLSEMRLEKDKFSINLDVKHFSPEELKVKVSGDFIEIHGKHEERQDEHGYVSRDFQRRYKIPVDVDPLSITSSLSPDGVLTVCGPRKQGDVPERSIPITRR",
    "response#3": "RLPSWIESGLSEMRLEKDKFSINLDVKHFSPEELKVKVSGDFIEIHGKHEERQDEHGYVSRDFQRRYKIPVDVDPLSITSSLSPDGVLTVCGPRKQGDVPERSIPITRR"
  },
  {
    "reference": "MASIVLRKTGERIEGNDKVGAFLNEQGVLFEHWNTDKIPAELAGRCNLNDEEKQQTLQVFEDDIKSLAERRGYVNWDVVALTESTPNLDELLKKFEEVHTHTEDEVRAITAGHGIFIIKGQGDTGYFDVELEAGDVISVPENTPHFFTLMQDRQVVAVRLFIEPAGWIAQPFQDPEFAK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must have high specificity towards nickel cation binding.2. Ideal protein should have a binding affinity for key enzymes in the L-methionine biosynthesis via salvage pathway, Amino-acid biosynthesis pathway to enhance the overall efficiency of the pathway.3. The protein must exhibit the following characteristics: Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.; Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site.4. We need a protein that can perform 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-   methylsulfanyl-2-oxobutanoate + formate + 2 H(+) with high efficiency. The designed protein sequence is ",
    "response#1": "MKSVSIVLVAAGQGARMGGGIPKQYRTLAGKAVLARSLAAFAGHPRIDAVQVVIAPGDEELYAAAVAGLDLPPPVAGGATRRDSVRAGLEALAGDPPDYVMIQDAARPFVDAALIGRVIDALATGSSAAAPGLAVTDTLKRVDGDLVTGTVERAGLWRAQTPQGFRFADILAAHRQAAGAELTDDLAVAEWAGLAVALVEGDPMNIKLTHPEDFARAEAMLAGRPDVPPPGPAPDPGPVHLV",
    "response#2": "MEAILTQPLITTIVVGAITAFIGAVVGFASMRLSIIGDALSHSVLPGIVIAYLLTGSKDSLWMLIGAVVFGVLTSLVIAWMRSRGRVGDDTSIGIVFTSMFALGVVLISRMGQNSAGLSQYLFGNILAVTPSDVTITALITVVVLISIIGFWRPLLAVSFNPEFAQTIGLPVKAVQFLLLLLLALVVVIGLQTVGVILMAALLITPGATARLWSERLGTMIVLAALFGAVSGAIGAVASYYFDASTAGAIVLIQTVLFLAVLLFSPRHGILARWSQR",
    "response#3": "MPKLLILAAGSGTRLGFEQPKAFVELLGHTVLHWSLNAFASVADIDQVVVVIPEDHVDVARDLLSRYAANRPVRTVVGGGERRQDSVRLGLAALPGDCDLVAVHDGARPCLTPALIDRCLEAARHSGAAIPAIPVADTVKRVSGSAVTATVDRSELVRVQTPQFFRRDDLVAAYARAAGEPTDEPTDDAQLLEAVGHSVRVVAGDPLAFKITTPADLERARDLLTRGKRPAGARDGEVVAPERSDAADSR"
  },
  {
    "reference": "MAHVRHFRTLLSGFYFWEAALLLSLVATKETNSARSRSAPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVTCNIFINSFGSIAETTMDYRVNIFLRQKWNDPRLAYSEYPDDSLDLDPSMLDSIWKPDLFFANEKGANFHEVTTDNKLLRIFKNGNVLYSIRLTLTLSCPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQDEAPVQVAEGLTLPQFLLKEEKDLRYCTKHYNTGKFTCIEVRFHLERQMGYYLIQMYIPSLLIVILSWVSFWINMDAAPARVALGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRKNKTEAFALEKFYRFSDTDDEVRESRLSFTAYGMGPCLQAKDGVVPKGPNHAVQVMPKSADEMRKVFIDRAKKIDTISRACFPLAFLIFNIFYWVIYKILRHEDIHHQQD",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have a binding site that is specific to the target strychnine, Zn(2+) ligand.2. For general function, the protein need meet that Channel characteristics depend on the subunit composition; heteropentameric channels display faster channel closure; Channel opening is triggered by extracellular glycine; Plays a role in cellular responses to ethanol.3. The protein should have Helical; Name=3, Helical; Name=4, Helical; Name=2, Helical; Name=1 domains to ensure proper insertion into the membrane.4. The ideal protein would be one that plays a critical role in nervous system process, neuropeptide signaling pathway through excitatory extracellular ligand-gated ion channel activity, glycine-gated chloride ion channel activity within presynaptic membrane, glycine-gated chloride channel complex, perikaryon, neuron projection. The designed protein sequence is ",
    "response#1": "DKLMGRTSGYDARIRPNFKGPPVNVTCNIFINSFGSVTETTMDYRVNIFLRQKWNDPRLAYSEYPDSSLDLDPSMLDSIWKPDLFFANEKGANFHDVTTDNKLLRIFKDGTVLYSIRLTLILSCPMDLKNFPMDVQTCTMQLESFGYTMNDLIFEWLDKGPVQVADGLTLPQFLIRDEKDLGYCTKHYNTGKFTCIEVKFHLERQK",
    "response#2": "RVVAFASLFFILVSITTFCLETHEAFNIDRNVTEIHRVGNITSVRFRREVETEPILTYIEGVCVMWFTLEFLVRIVCCPDTLDFVKNLLNIIDFVAILPFYLEVGLSGLSSKAARDVLGFLRVVRFVRILRIFKLTRHFVGLRVLGHTLRASTNEFLLLIIFLALGVLIFATMIYYAERIGARPSDPRGNDHTDFKNIPIGFWWAVVTMTTLGYGDMYPKTWSGMLVGALCALAGVLTIAMPVPVIVNNFGMYYLASKIWTPDTFFHNGKKSVAHNMTTPNKLLRLVDNGTLLYTMRLTIHAECPMHLEDFPMDVHACPLKFGSYAYTKTEVIYTWTLGKVEVAEGGSRLNQYDLLGHNVGRDSIESSTGTYIVMTTYFHLKRKSNILNRLLMTYDSRIRPNFKGIPVEDKVNIFINSFGSIQETTMDYRVNIFLRQRWNDPRLRLPTDFKSDALTVDPKMFQCLWKPDLFFANEKNANFHDVTQENILLFIFRNGDVLISMRLSVTLSCPLALQLFPMDTQYCKMQLESFGYTTKDLVFMWQSGDPVQMDEIALPQFDVKQEDIKYANCTKFYPGTGYYTCVEVIFTLRRR",
    "response#3": "LLAILANMGFMISFGIRCNFGAAKTHMYKNYTDPYGKVHMHEFNWTIDELSVMESSYFYGYLVTQIPAGFLAAKFPPNKLFGFGIGVGAFLNILLPYGFKVKSDYLVAFIQITQGLVQGVCYPAMHGVWRYWAPPMERSKLATTAFTGSYAGAVLGLPLSAFLVSYVSWAAPFYLYGVCGVIWAILWFCVTFEKPAFHPTISQEEKIFIEDAIGHVSNTHPTIRSIPWKAIVTSKPVWAIIVANFARSWTFYLLLQNQLTYMKEALGMKIADSGLLAAIPHLVMGCVVLMGGQLADYLRSNKILSTTAVRKIFNCGGFGGEAAFMLIVAYTTSDTTAIMALIAAVGMSGFAISGFNVNHLDIAPRYAAILMGFSNGIGTLAGLTCPFVTEAFTAHSKHGWTSVFLLASLIHFTGVTFYAVYASGERQHWGETAETHDKLMGRTSGYDARIRPNFKGPPVNVSCNIFINSFGSIAETTMDYRVNIFLRQQWNDPRLAYNEYPDDSLDLDPSMLDSIWKPDLFFANEKGAHFHEITTDNKLLRISRNGNVLYSIRITLTLACPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQEQGAVQVADGLTLPQFILKEEKDLRYCTKHYNTGKFTCIEARFHLERQE"
  },
  {
    "reference": "MTKYIFVTGGVVSSIGKGIAAASLGRLLKNRGLNVTIQKFDPYINVDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFIDINLNQYSNVTTGKVYSEVLNKERRGDYLGATVQVIPHITDEIKNKIMRAAETTEADVVITEVGGTVGDIESLPFLEALRQMKRDVGSDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRSLGIQPNVLIVRSEYPVGQHLKDKLALFTDVDASAVIESLDVETLYTIPLNLQKQGLDQIVVDHLKLDVNEADMTEWRALEEKVLNLKNQTRIAIVGKYVELPDAYLSVVEALKHAGYAVDSDIDIKWVNAELLSPKEVVQELQDVDGVLVPGGFGERGLEGKMLAIRYARENNVPFFGICLGMQLAAIEYARNVAGIEGAGSAEVNPDVEHNIIDLMPDQKDVVEMGGTLRLGSYPCELKKGSVTAEAYANADVIDERHRHRYEFNNHYRNDLEAAGLIFSGVSPDNRLVEIIEVADHSYFVGCQFHPEFKSRPNRPHPLFVSFVKKALDLRK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The active site active site of the designed protein must be optimized for substrate binding and specificity.2. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.. The designed protein sequence is ",
    "response#1": "LPDAYKSIIEAFIHAGAQNECKVKLKTFHAELIDKETVASQLQNLDGILVAPGFGQRGFEGKIQTICYARENNIPFFGICLGMQAAAIEFARNVLNIKDATSTEMEPEAKNPVIDMMADQKEITQMGGTMRLGSYTCELKKGSKAFSAYGKNKITERHRHRYEFNNKYLQAFEENGMIASGINPETGLVEVIELQNHPWFVAAQYHPELKSTVQNPHPLFVKFIAS",
    "response#2": "VTIAIISKYGIEDVYMSIKEALKHAGRATSTEVIIRWLDAEDCTSADLLDVDGILIPGGFGIRGIEGKIRAIRHAREKKIPLFGICLGFQLAVIEYARHVVGLADATSSEMGSGTHVITLLPEQEDETNFGGTMRLGDYPVVIIPKTSAEKIYGAVEITERHRHRYEVNPEYIPALEEKGVVFSGRNNARMEIMEIPDHPFFLATQFHPEFRSRPTRPSPPFLEFIR",
    "response#3": "LIDSYKSLAEALTHGGIANNVKVKINWLDSEIFEEDGAIQHLEDVHGILVPGGFGERGSEGKIKAAGFARERQVPYFGICFGMQMAVIEAARSLAGFPGAGSSEFGTPKDPIVGLLTEWTRGNRVERRDNDSELGGTMRLGAYSCILRDGSRVLDIYGEREITERHRHRYEVNINYREALERSGLTFSGLSPDGELPEIVELADHPWFIGVQFHPELKSKPFDPHPLFTAFIAR"
  },
  {
    "reference": "MGFMRKADFELYRDADKHYNQVLTTRDFLALGVGTIISTSIFTLPGQVAAQFAGPGVVFSYLLAALVAGFVALAYAEMSTVMPFAGSAYSWISVLFGEGFGWIAGWALLAEYFIAVAFVGSGFSANLQQLLAPLGFQLPKVLANPFGTDGGIVDIISLLVILLSAIIVFRGASDAGRISQILVVLKVAAVIAFIIVGITVIKPANYHPFIPPHNPKTGFGGFSGIWSGVSMIFLAYIGFDSIAANSAEAKNPQKTMPRGIIGSLLIAVVLFAAVTLVLVGMHPYSAYAGNAAPVGWALQQSGYSVLSEVVTAIALAGMFIALLGMVLAGSRLLYAFGRDGLLPKGLGKMNARNLPANGVWTLAIVAIVIGAFFPFAFLAQLISAGTLIAFMFVTLGIYSLRRRQGKDLPEATYKMPFYPVLPALGFIGSLFVFWGLDVQAKLYSGIWFLIGIAIYFAYGNRRKKK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should be expressed specifically in the plasma membrane2. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "MNFLELQVTLSFCVIFLLRMLGMFMILPILSKYGMLLDGGNKFLIGLSMGIYGISQVIFQIPFGILSDKFNRKKIILLGLFMFFIGNIISASIHSIWGLIIGRFFQGSGAISGVCMAFLSDLIREENRVKSIAAIGVSFAISFLIAVVSGPIIVHYFGFFSIFWISAFLSIVCMIIVCFFVPFSKKNILKQNKTLHSYKKVLNFVLNKVFFRFYLGVFFLHFLLMIKFTMIPNQFEISGFSLDNHWKVYLGTILISFFVLFLFIFYCKYKYILENIIEICILFILFSEIIFLSAQKNLLFLIISLQIFFISFNFLEVFLSSHLSRQLSNNYRGSIMSIYSTSQFLGIFFGGVFSGWLYSFLNFSQIFYFELFIILLWLIFSFFLRNRFY",
    "response#2": "MNFLELQVTLSFCVIFLLRMLGMFMILPILSKYGMLLDGGNKFLIGLSMGIYGISQVIFQIPFGILSDKFNRKKIILLGLFMFFIGNIISASIHSIWGLIIGRFFQGSGAISGVCMAFLSDLIREENRVKSIAAIGVSFAISFLIAVVSGPIIVHYFGFFSIFWISAFLSIVCMIIVCFFVPFSKKNILKQNKTLHSYKKVLNFVLNKVFFRFYLGVFFLHFLLMIKFTMIPNQFEISGFSLDNHWKVYLGTILISFFVLFLFIFYCKYKYILENIIEICILFILFSEIIFLSAQKNLLFLIISLQIFFISFNFLEVFLSSHLSRQLSNNYRGSIMSIYSTSQFLGIFFGGVFSGWLYSFLNFSQIFYFELFIILLWLIFSFFLRNRFF",
    "response#3": "MNFLELQVTLSFCVIFLLRMLGMFMILPILSKYGMLLDGGNKFLIGLSMGIYGISQVIFQIPFGILSDKFNRKKIILLGLFMFFIGNIISASIHSIWGLIIGRFFQGSGAISGVCMAFLSDLIREENRVKSIAAIGVSFAISFLIAVVSGPIIVHYFGFFSIFWISAFLSIVCMIIVCFFVPFSKKNILKQNKTLHSYKKVLNFVLNKVFFRFYLGVFFLHFLLMIKFTMIPNQFEISGFSLDNHWKVYLGTILISFFVLFLFIFYCKYKYILENIIEICILFILFSEIIFLSAQKNLLFLIISLQIFFISFNFLEVFLSSHLSRQLSNNYRGSIMSIYSTSQFLGIFFGGVFSGWLYSFLNFSQIFYFELFIILLWLIFSFFLRNRFY"
  },
  {
    "reference": "MIGRLFGNVSQRVPSLLYRVSLMMKPQVVFVLGGPGAGKGTQCSKIVESYNYTHLSAGDLLRAERAREGSEFGQLIGNYIKEGKIVPVEITINLLKKAMEETMQKDEKKFRFLIDGFPRNEDNLQGWNNVMDGKADVKFVLFFDCGNEVCIDRCLERGKNSGRTDDNRESLEKRIQTYLQSTQPIIKLYEKHGKVRTVDASRSVDKVFADVKAILDKEG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have UMP kinase activity, dCMP kinase activity within nucleus, cytoplasm to regulate phosphorylation, 'de novo' pyrimidine nucleobase biosynthetic process, pyrimidine nucleotide biosynthetic process.2. For general function, the protein need meet that Plays an important role in de novo pyrimidine nucleotide biosynthesis3. The protein should have a low Km value for a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-   triphosphate + ADP.4. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding. The designed protein sequence is ",
    "response#1": "ECTLAMIKPNTALLHQDKILAIVAAQGYKVVCKSQATLTEGQASEFYGEHRGKPFFPALLQYMTSGETVALLLQREGAIKGWRALMGPTNSLKAREEAPQSIRGMFGVDGTMNATHGSDAPLSAAREVMFHFR",
    "response#2": "ECTLAMIKPNTALLHQDKILAIVAAQGYKVVCKSQATLTEGQASEFYGEHRGKPFFPALLQYMTSGETVALLLQREGAIKGWRALMGPTNSLKAREEAPQSIRGMFGVDGTMNATHGSDAPLSAAREVMFHFR",
    "response#3": "ECTLAMIKPNTALLHQDKILAIVAAQGYKVVCKSQATLTEGQASEFYGEHRGKPFFPALLQYMTSGETVALLLQREGAIKGWRALMGPTNSLKAREEAPQSIRGMFGVDGTMNATHGSDAPLSAAREVMFHFR"
  },
  {
    "reference": "MNPAALKCDSRRVQPGDTFVAVRGAADDGSRFIAAAIEAGAAEIVTELPRPAEGVPDGVAWRTVEDSRREIAALACRAYGDPSAAIDVFGVTGTNGKTTTATLLRKILNDCGIPSGLVSTISYITAPPLPPPALLGLLGRAPVEAKEIPAHNTTPGPLELQSLFAEMRDNGCKAAVMEVSSHAIAQRRVEGTRFAAVAFTNLTQDHLDYHGTMEAYFEAKRQLFVPARFPAAINLDCPYGRRLFDAIKSDGGAPALSYGAASDADVRFSNERLSSLGCEFRLDYPGGSAQVRLRLLGRHNIHNALCAFATALLHGIPARMALDSLATAKPVRGRLEMVRIKASPATYFIDYAHTPDAIENVLNTLREITEGRLFIVFGAGGDRDRRKRPLMGEAAARLADVCIVTSDNPRSEDPAAIIEDILAGMPEPEQPSEPPAAEPGHSASAPGVLRPSPPWLGQPGAERTAALGQPDAMRPACSRPAVLIEPDRRAAIRRTVLLADQPGDVVLIAGKGHETYQIFADRTDHFDDREELISWGS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that can modulate the overall flux of metabolites through the Cell wall biogenesis pathway, enhancing or suppressing its activity as needed.2. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "IMSATQTTVPESEKAVGGHLREVGLTFHFFNQLPAIIADNVGNSLAEAFEPIGIKDWNNIFWVAHPGNWAIMDAIETKLGLEQSKLATARHVFSEFGNMQSATVYFVMDELRKRSAAENRATTGDGLRWGVLFGFGPGISIETVVLQSVV",
    "response#2": "IMSATQTTVPESEKAVGGHLREVGLTFHFFNQLPAIIADNVGNSLAEAFEPIGIKDWNNIFWVAHPGNWAIMDAIETKLGLEQSKLATARHVFSEFGNMQSATVYFVMDELRKRSAAENRATTGDGLRWGVLFGFGPGISIETVVLQSVI",
    "response#3": "IMSATQTTVPESEKAVGGHLREVGLTFHFFNQLPAIIADNVGNSLAEAFEPIGIKDWNNIFWVAHPGNWAIMDAIETKLGLEQSKLATARHVFSEFGNMQSATVYFVMDELRKRSAAENRATTGDGLRWGVLFGFGPGISIETVVLQSVL"
  },
  {
    "reference": "MASADDYFSDFEDDELDKLYEKAINKSVKETITRRAVPVQKDLHDNVLPGQKTVYEEIQRDVSFGPTHHELDYDALSFYVYPTNYEVRDYQYTIVHKSLFQNTLCAIPTGMGKTFIASTVMLNYFRWTKKAKIIFTAPTRPLVAQQIKACLGITGIPSDQTAILLDKSRKNREEIWANKRVFFATPQVVENDLKRGVLDPKDIVCLVIDEAHRATGSSAYTNVVKFIDRFNSSYRLLALTATPASDLEGVQEVVNNLDISKIEIRTEESMDIVKYMKKRKKEKIEVPLLLEIEDIIEQLGMAVKPVLQQAIELGIYEECDPSQINAFKAMQQSQKIIANPTIPEGIKWRNFFILQLLNNVGQMLKRLKIYGIRTFFNYFQNKCTEFTTKYNLKKSTNKIAAEFYYHPILKNIKNQCENYLSDPKFVGHGKLQCVRDELMDFFQKRGSDSRVIIFTELRESALEIVKFIDSVADDQIRPHIFIGQARAKEGFDEVKYTRKHAPKGRKKVERLHRQEQEKFLEAERTKRAANDKLERSARRTGSSEEAQISGMNQKMQKEVIHNFKKGEYNVLVCTSIGEEGLDIGEVDLIICYDTTSSPIKNIQRMGRTGRKRDGKIVLLFSSNESYKFERAMEDYSTLQALISKQCIDYKKSDRIIPEDIIPECHETLITINDENEIINEMEDVDEVIRYATQCMMGKKVKPKKAITKKKRVQENKKPKKFFMPDNVETSIVSASTLINKFLVNESGGKQLVTSNENPSKKRKIFKAL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. We need a protein that can perform ATP + H2O = ADP + H(+) + phosphate with high efficiency.2. For general function, the protein need meet that ATP-dependent DNA helicase involved in DNA damage repair by homologous recombination and in genome maintenance; Component of a FANCM-MHF complex which promotes gene conversion at blocked replication forks, probably by reversal of the stalled fork (Probable); Plays a role in limiting crossover recombinants during mitotic DNA double-strand break (DSB) repair3. The designed protein should have four-way junction DNA binding, DNA/RNA helicase activity, ATP hydrolysis activity, four-way junction helicase activity within nucleus to regulate recombinational repair, double-strand break repair via synthesis-dependent strand annealing. The designed protein sequence is ",
    "response#1": "MAAVPLNNLQEQLQRHSARKLNNQPSLSKPKSLGFTFKKKTSEGDVSVTSVSVVKTPALSDKDVNVSEAFSFTESPLHKPKQQAKIEGFFKHFPGRQQSKGTCSEPSLPATVQTAQDTLCTTPKTPTAKKLPVAVFKKLEFSSSADSLSDWADMDDFDMSASDAFASLAKNPATRVSTAQKMKKTKRNFFKPPPRKANAVKTDLTPPSPECLQVDLTKESEEEEEEEEEAEGADCLSRDVICIDNDSASEELTEKDTQESQSLKAHLGAERGDSEKKSHEDEAVFHSVQNTEYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKGILSTSEELLSKPEEMTTHKSDAGTSKDCDAQQIV",
    "response#2": "RPNSFKEFIGQEQIKSSLKIAIEASKYRKEALEHTLLYGQPGLGKTTLALLISYEMNTKCKVASAPSLERPRDIVGLLLGLKEGEILFIDEIHRLNKLTEELLYSAMEDFRLDLTMGANRGTRCRTINLPKFTLIGATTKLASISAPLRDRFGLCHM",
    "response#3": "GSLWRHRPDSLDNTVQGDSCPVGHPNKELNSPYLLSHSPSTEECLPTTTPGKTGFSATPKNLFERPLLNSHLQKSFVSSNWAETPRMENRNESTDFPGSVLTSTTVKAQSKQAASGWNVERHGQASYDIDNFNIDDFDDDDDDDDWENIMHNFPASKSSTATYPPIKEGGPVKSLSERISSAKAKFLPVVSTAQNTNLSESIQNCSDKLAQNLSSKNPKHEHFQSK"
  },
  {
    "reference": "MNPVAVTSVAPVDIIHDLRHSEHATEASPITLLHVFSRAVSQYPNHELNFITSSAHDSIHTKTFTEFDQYVRALAQAMLAWGKPAGSVIVVYLTEHEDNMAAIWASLFAGYVPCLQPALSAQQAHKEGHVAHIKNLFSSATWLTNESGAEQVQSIPGLDIHLLSELQASAETVSVDFQTHQPHLDDEAILFLTSGSTGFSKAVVHTHRTILAGCNAKGQSYGLTSESKIMNWVGFDHVVGSLGMHITPLLCGASQLHVHASAILSDSLRFLHLIEEKSIQLVFAPNFLLAKLTRDLEKRSDLFGKFDLSSIKRINSGGEAVVSSTAQAFARTLKNLAKDGDASFVFSTGFGMTETGAGCIYDTIDVLGTSPPHEFLEIGTPVAGCEMRIVNPEDGVTPRPDGESGELQVRGPMVFVRYYNNPETTSSSFVEGGWYRTGDVGIFEKGKMRLSGRIKDTVVVHGVSYGIPELETYLQTVEGVAHSFLVAAPYRAPGQETEGFVVFYSPTFDLDSEDAPAKLYATHRALRDVSVKLITLPPQQIIPLTLNQMEKSTLGKLSRARLLNLFKQGELAKYIIRAEELLGIARGANFVAPSTETEKTLAGIYAGIFHLSVSDISTSENFFEFGGTSIDAIRLKREAESAFDLSEIPTIQIFKHPEIITLAKYVDSLVSKDASEEEYDPIVPLQLTGKKTPIFMVHPGIGEVLIFVNLAKYFQNERPFYALRARGFEAGQPCFTSLDEMVSCYAAAIKRTQPHGPYAIAGYSYGGV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Does not accept 4- hydroxyphenylpyruvate (4-HPP) as substrate..2. The designed protein must be able to perform phosphopantetheine binding, transferase activity under specific conditions.3. The presence of Carrier domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "VGVIAGSEGMAGAPYLTSKSALRTGSGLVYSIVPKSIFTISQIKNTEVIVKSFECLSDIMAHSKDIDVVALGPGIGVNQNTIEMVKHILENLKKPIVLDADGINCISQCRDVLLSRNETTIITPHPGEMSRLINVDISEIQKNREKYSMEVAKRYGVIVVLKGSGTVVCDGKDIYINTTGNPGMSTSGSGDVLTGVIASLLGQGIGALDAAKLGAYLHGLAGDIAKEEKGEYGIIASDIIDYIK",
    "response#2": "IGDIVISGLSGKLPESSNIAEFRHNLLNGIDMVTNDPRRWEAGIYGLPERMAKMKDEDLEKFDDKFFSVHQKQAELMDPCMRMLLELTHEAIIDAGINPVELRGSRTGVYIGLSFVETEHEIPNMEPSSINGYCLTGCARAMFANRISYTFDFKGPSFIVDTACSSSLVALAHAYDDMRSGRCDYAVVAGVNLILKPIFALQFLRLGIVSQDGACKTFDAAANGYARADTCAVVLLQRSEHAKRVYATLLNVRTNTDGFKEQGVTFPDGRMQLQLLKETYSEIGLSPDEVVYVEAHGSGTPVGDDQEANMLSNFFCHSKRQSPLLIGSVKSNMGHAEPASGVSALAKMIIAMEEGVIPSNLHYKTPNPAVPALVQGRLKVVSRNTPWQGGIVGLNSFGFGGANAHVILKPNSRQPRSSGVDIVDVYRIENIINNKRDKFYKKVFTLIEKKYIEENNHNPQTIAGMFAAKEAISKLLGTGIGKVNFHHIEILHDENKKPYINLYDEGLKVCSQLGIDNINLSISHEKKYAIAFAPESNKGCYGRLLCVCGSEGMAGAAAMSTLSALRCGTGIVETALPRSIYPIVASLAPEAVFTLLDASPGGDLADRDLESLDSALSRASACLVGCGLGKSPFARRTVSRVLQTADVPLVLDADGINIVSEHIDELKTVRAPLVLTPHPGEMARLLRTTPEEVQKNRELFARSFARDLGVILVLKGFETLVVSPQGKLYRNTTGNPGMAKGGSGDVLAGMIASFAAQGVDLFSAAAGAVYLHGLAGDRCAGTLSQCAMLPTDLIGKL",
    "response#3": "MIKGIGIDIIEIDRIKSIVSKNKRFLQKIFTESEIEYIKSRNYNSNTISGLFSSKEAISKVLGTGISGFNWTDIEIIHDQLGKPRVKLKGNAKIISDNKNIDQILLSISHSNKDAISVAIGE"
  },
  {
    "reference": "MSTFKAPKGTYDLIPPDSAKYLAVREAIAAPLRNSGYGYIETPGFENVELFARGVGESTDIVTKEMYAFETKGGDRLALRPEGTASVLRAALEANLHKAGNLPVKLWYSGSYYRYERPQKGRYRHFSQVGAEAIGAEDPALDAELIILADQSYRSLGLRNFRILLNSLGDKECRPVYRAALQDFLRGLDLDEDTLRRAEINPLRVLDDKREDVQKQLGGAPLLRDYLCDACKAYHEEVRELITAAGVSFEDDAKLVRGLDYYTRTTFEFVHDGLGSQSAVGGGGRYDGLSEMIGGPALPSVGWALGVDRTVLALEAEGVELELPSSTSVFAVPLGEEARRILFAKVTELRKVGIAADFAYGGKGLKGAMKNANRSGARYTVVAGERDLAEGVVQLKDMESGEQTAVGVNEIVAELEARLG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit ATP binding, histidine-tRNA ligase activity. The designed protein sequence is ",
    "response#1": "CLVPIGREAEEHALTLANELRRNGLYVIYEYNGALKNRMKKASQANAKAALIFGNEELGSKTLKIKNMDTGEEKIIAYGNIMENIK",
    "response#2": "CLVPIGREAEEHALTLANELRRNGLYVIYEYNGALKNRMKKASQANAKAALIFGNEELGSKTLKIKNMDTGEEKIIAYGNIMENIK",
    "response#3": "CLVPIGREAEEHALTLANELRRNGLYVIYEYNGALKNRMKKASQANAKAALIFGNEELGSKTLKIKNMDTGEEKIIAYGNIMENIKP"
  },
  {
    "reference": "MSIRYIDEISDLREKKIFMRVDFNVPLDEHQNITEDTRIRAVLPTINYALDYNAKVVLASHLGRPKGERNAKYSMAPAAKRLSRLLGKEVKLAPDCIGDGVSKIIDSMQPGDVVMLENLRFYPGEEKNDDDFAKALADHCDIYVNDAFAVSHRAHASVEAITKFFPIVAAGFLMKNEMSYFEKSMKNPIRPLVAILGGAKVSGKLEVLENLCNKVDKVIIGGGMAFTFLKALGYNVGKSLVEENLLETALNTYNKAREKGIKFYLPVDCVVADQFNPAAETKVTTIQEIPEGWMALDIGPATVTLFSTALQNAKTIVWNGPMGVFEMDAFSRGTFAMVSAVANSYALTIVGGGDTDVAVHRAGEYAKISYISTGGGAFLELLEGKKLPGIKVLEDNGHK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that can modulate the overall flux of metabolites through the pyruvate from D- glyceraldehyde 3-phosphate: step 2/5 pathway, enhancing or suppressing its activity as needed.2. The protein should have phosphoglycerate kinase activity and be active in cytoplasm. The designed protein sequence is ",
    "response#1": "YAHSGGVTAVINASAAAVVAAARASGRVGKVFAAWRGIVGVLEEALIDTAGLDEAALVRLARTPGGAFGSCRFDLPHDDGGAAMDRLFAVFDAHDIGWLLYNGGNGSMDAVARIQAEAARRGHALVCVGVPKTVDNDIVGTDCCPGFGSAARYLATSMLEAGLDTASMVGSKGSVFVMEVMGRNTGWLAAATALAAQGDPDRAPHIVLVPEARFDEAAFLARVREIVERLGYCAVTVAEGIRRPDGSLLMEKSRDARGYVQLGGAGLAVARRINERLGYKFHWAIPDYLQRSAGHWLSATDRAQAEAVGRVAVELAWAAGHGAM",
    "response#2": "YAHSGGVTAVINASAAAVVAAARASGRVGKVFAAWRGIVGVLEEALIDTAGLDEAALVRLARTPGGAFGSCRFDLPHDDGGAAMDRLFAVFDAHDIGWLLYNGGNGSMDAVARIQAEAARRGHALVCVGVPKTVDNDIVGTDCCPGFGSAARYLATSMLEAGLDTASMVGSKGSVFVMEVMGRNTGWLAAATALAAQGDPDRAPHIVLVPEARFDEAAFLARVREIVERLGYCAVTVAEGIRRPDGSLLMEKSRDARGYVQLGGAGLAVARRINERLGYKFHWAIPDYLQRSAGHWLSATDRAQAEAVGRVAVYAHSGGVTAVINASAAAVVAAARASGRVGKVFAAWRGIVGVLEEALIDTAGLDEAALVRLARTPGGAFGSCRFDLPHDDGGAAMDRLFAVFDAHDIGWLLYNGGNGSMDAVARIQAEAARRGHALVCVGVPKTVDNDIVGTDCCPGFGSAARYLATSMLEAGLDTASMVGSKGSVFVMEVMGRNTGWLAAATALAAQGDPDRAPHIVLVPEARFDEAAFLARVREIVERLGYCAVTVAEGIRRPDGSLLMEKSRDARGYVQLGGAGLAVARRINERLGYKFHWAIPDYLQRSAGHWLSATDRAQAEAVGRVAVERAGDPGRVLSNAEYAHSGGVTAVINASAAAVVAAARASGRVGKVFAAWRGIVGVLEEALIDTAGLDEAALVRLARTPGGAFGSCRFDLPHDDGGAAMDRLFAVFDAHDIGWLLYNGGNGSMDAVARIQAEAARRGHALVCVGVPKTVDNDIVGTDCCPGFGSAARYLATSMLEAGLDTASMVGSKGSVFVMEVMGRNTGWLAAATALAAQGDPDRAPHIVLVPEARFDEAAFLARVREIVERLGYCAVTVAEGIRRPDGSLLMEKSRDARGYVQLGGAGLAVARRINERLGYKFHWAIPDYLQRSAGHWLSATDRAQAEAVGRVAV",
    "response#3": "YAHSGGVTAVINASAAAVVAAARASGRVGKVFAAWRGIVGVLEEALIDTAGLDEAALVRLARTPGGAFGSCRFDLPHDDGGAAMDRLFAVFDAHDIGWLLYNGGNGSMDAVARIQAEAARRGHALVCVGVPKTVDNDIVGTDCCPGFGSAARYLATSMLEAGLDTASMVGSKGSVFVMEVMGRNTGWLAAATALAAQGDPDRAPHIVLVPEARFDEAAFLARVREIVERLGYCAVTVAEGIRRPDGSLLMEKSRDARGYVQLGGAGLAVARRINERLGYKFHWAIPDYLQRSAGHWLSATDRAQAEAVGRVAVETLRRAG"
  },
  {
    "reference": "MANPWWVGNVAIGGVESPVTSSAPSLHHRNSNNNNPPTMTRSDPRLDHDFTTNNSGSPNTQTQSQEEQNSRDEQPAVEPGSGSGSTGRRPRGRPPGSKNKPKSPVVVTKESPNSLQSHVLEIATGADVAESLNAFARRRGRGVSVLSGSGLVTNVTLRQPAASGGVVSLRGQFEILSMCGAFLPTSGSPAAAAGLTIYLAGAQGQVVGGGVAGPLIASGPVIVIAATFCNATYERLPIEEEQQQEQPLQLEDGKKQKEENDDNESGNNGNEGSMQPPMYNMPPNFIPNGHQMAQHDVYWGGPPPRAPPSY",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Binds the DNA sequence GNFEI (GA-negative feedback element I) in the GA3OX1 promoter; Negatively regulates plant innate immunity (PTI) to pathogens through the down-regulation of the PAMP-triggered FRK1 expression.; Transcription factor that specifically binds AT-rich DNA sequences related to the nuclear matrix attachment regions (MARs) 2. The protein design should emphasize Polar residues, Basic and acidic residues compositional bias.3. The designed protein should possess a A.T hook domain that is capable of recognizing and binding to DNA in a specific orientation and spacing.4. The ideal protein would be one that plays a critical role in negative regulation of innate immune response through minor groove of adenine-thymine-rich DNA binding within nucleus. The designed protein sequence is ",
    "response#1": "PHVISVGSGEDIVSKVLSFSQKRPRALCIMSGTGTVSSVTLREPASTTPSLTFEGRFEILSLGGSYLVNEEGGSKSRTGGLSVSLSGPEGHVIGGGIGMLIAASLVQVVACSS",
    "response#2": "EKDEEFVCISCVEEVRYSFVSHLSEALRRKGINNVVVDVDIDDLLFKESQAKIEKAGVSVMVLPGNCDPSEVWLDKFAKVLECQRNNKDQAVVSVLYGDSLLRDQWLSELDFRGLSRIHQSRKECSDSILVEEIVRDVYETCV",
    "response#3": "EKDEEFVCISCVEEVRYSFVSHLSEALRRKGINNVVVDVDIDDLLFKESQAKIEKAGVSVMVLPGNCDPSEVWLDKFAKVLECQRNNKDQAVVSVLYGDSLLRDQWLSELDFRGLSRIHQSRKECSDSILVEEIVRDVYETL"
  },
  {
    "reference": "MNNFGRNLALWVIVALLLVALFNLFQPSGTNQARGQQVAYSDFLNEVQAGRVRDARIQGRVVSGQLTDGRTYSTYVPDDPALVGKLTDKGVRVEARPEESDVNPLFHYLLSWFPMLLLIGVWVFFMRQMQGGGGRAMGFGKSRAKLLTEKQGRVTFDDVAGIDEAKAELEEIVEFLRDPQKFQRLGGKIPKGVLLVGPPGTGKTLLARSIAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLNQMLVEMDGFESNEGVILIAATNRPDVLDPALLRPGRFDRQVVVPNPDVNGREKILRVHMRKVPLASDVDPKVIARGTPGFSGADLANLVNEAALLAARIGRRTVGMLEFEQAKDKVLMGAERRSLVMSEAEKRMTAYHEAGHALVALSEEECDPVHKATIIPRGRALGLVMSLPEGDRYSKHKSKLKAELAMAMGGRVAEEIIFGPDKVSNGASGDIKMATDQARRMVMEWGMSDRLGMIAYGSNDQEVFLGHSVTQTKNISEATAREIDAEIRGIIDAAYARATKILTENIESLHALAKGLLEYETLSGDEIRTLLRGEPVVRNRPDEPVNQGRGSVPTSGRSTPPRPAGPWQNPAPSGA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "EARQPELMNALQTKMFLLAGLII",
    "response#2": "MKTF",
    "response#3": "MRERR"
  },
  {
    "reference": "MLNTIEEAVKDLQAGKVIILVDDESRENEGDFVCLAEHATPENINFMATHGRGLICTPVSPDIALRLKLDQMVSHNTDHHETAFTVSIDHKDAKTGISAFERSDTILQMLDTKAVPQDFKRPGHVFPLVAKSGGVFQRQGHTEASVDLARLSGSEPAAVICEIMNIDGSMSRMPELEKLADEFNLKLITIEDLYAYRAEKDPILKRESSVHMPTDFGEFRMVGYSNRLDTEEHVAIVKGNPESVEAPIVRIHSECLTGDIFHSRRCDCGAQLTKSLELIEQAGTGIVLYMRQEGRGIGLLNKLKAYELQEQGLDTVEANEQLGFPAEMRDYTLCALMLKDLGVSRVRLLTNNPAKTDALIEYGIEVEERLALVIPPTEDNTQYMKTKKQRMHHLID",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must be able to perform 3,4-dihydroxy-2-butanone-4-phosphate synthase activity under specific conditions.2. For general function, the protein need meet that Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate. The designed protein sequence is ",
    "response#1": "RKEEIHLPTEFGDFKLISYEQKDTKDIHIALKKGNWNENDEVLVRVHSSCVTGDILGSLRCDCGSQLKNALKMINDKGKGLLLYMNQEGRGIGLLNKLKAYKLQEEGYDTVEANEKLGFKMDDRDYGVGAQILNDLNIKKINLLSNNPIKRAGLLGYGLEISRG",
    "response#2": "REVTVKMPTEWGDFDMTAYTQLDTGENHLALVKGSWEPGEPVLVRVHSSCMTGDIFGSCRCDCGPQLHKAMEMISKEGKGAIVYMNQEGRGIGIINKLRAYHLQENGYDTVDANLKLGFPMDQRDYGIGAQILRNLGITKMRLMSNNPKKRAGLIGYGLEVVENVK",
    "response#3": "RKEEIHLPTEFGDFKLISYEQKDTKDIHIALKKGNWNENDEVLVRVHSSCVTGDILGSLRCDCGSQLKNALKMINDKGKGLLLYMNQEGRGIGLLNKLKAYKLQEEGYDTVEANEKLGFKMDDRDYGVGAQILNDLNIKKINLLSNNPIKRAGLLGYGLEISRG"
  },
  {
    "reference": "MRLILLGPPGAGKGTQAASIVEKYHIPHISTGDIFRYNIKQGTELGKKAKSYMDQGLLVPDEVVVEIVEDRLKKEDCENGFLLDGFPRTVVQAEALDKALVDMNISLDKVINIQVDKERLIERAVGRRICRECGATFHVQYNPSTKGALCDQCGGELYQRDDDNEETVTRRIEVYLSETTPLVEYYSSQNKLVTIDGDKKINEVFANIVTSLGSDL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must have high specificity towards zinc ion binding.2. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.3. A protein that can modulate the overall flux of metabolites through the AMP biosynthesis via salvage pathway pathway, enhancing or suppressing its activity as needed.4. For general function, the protein need meet that Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP; Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. The designed protein sequence is ",
    "response#1": "MLAYMNKESLEVTLKERKACYWSRSRQELWLKGATSGNFQEVMQISIDCDADAILLKVKQKGGACHVGYYSCFA",
    "response#2": "MLAYMNKESLEVTLKERKACYWSRSRQELWLKGATSGNFQEVMQISIDCDADAILLKVKQKGGACHVGYYSCFR",
    "response#3": "MLAYMNKESLEVTLKERKACYWSRSRQELWLKGATSGNFQEVMQISIDCDADAILLKVKQKGGACHVGYYSCFR"
  },
  {
    "reference": "MSAFRTLDDAGSLKGKRVLMRVDLNVPMESGRVTDATRIERVAPTIREVAEQGAKVILLAHFGRPKGKREPKDSLEPVVPALGAALGRDVAFAADCVGDAAAQAVAALKDGDVLLLENTRYHAGEEKNDAAFADALAANGDVYVNEAFSAAHRAHASTEGLAHRLPAYAGREMQAELDALTKGLESPQRPVIALVGGAKVSSKIDLLENLVAKVDMLVIGGGMANTFLHAQGKAVGKSLCEKDLAETATRILAAAEKAGCRIILPVDAVAASEFKARAPHETVPVDAVPDASMILDAGPRSVAEINAAIDGAKTLVWNGPLGAFELAPFDAATVATAKHAAARTKDGQLVSVAGGGDTVAALNHAGVGQDFTYVSTAGGAFLEWLEGKALPGVEALRAKG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein should be able to manipulate the activity of enzymes in the Carbohydrate degradation, pyruvate from D- glyceraldehyde 3-phosphate: step 2/5, glycolysis pathway, either by activating or inhibiting their function.2. The designed protein should have a binding site that is specific to the target ATP ligand.3. The designed protein must be able to regulate glycolytic process. The designed protein sequence is ",
    "response#1": "LTRTLSALQEYIDYVYAVMIRRAADPNDSKRGPVNAITPSVTVPSHHGPDVQTVIPINIETMAFVVPTTLMHVHSVMIELKKPITREDVIDIFENTTRVLLFEKERGFESTAQLIEFARDLHREWNNLYEIAVWKESISVKGNRLFYIQAQ",
    "response#2": "LTRTLSALQEYIDYVYAVMIRRAADPNDSKRGPVNAITPSVTVPSHHGPDVQTVIPINIETMAFVVPTTLMHVHSVMIELKKPITREDVIDIFENTTRVLLFEKERGFESTAQLIEFARDLHREWNNLYEIAVWKESISVKGNRLFYIQAR",
    "response#3": "LTRTLSALQEYIDYVYAVMIRRAADPNDSKRGPVNAITPSVTVPSHHGPDVQTVIPINIETMAFVVPTTLMHVHSVMIELKKPITREDVIDIFENTTRVLLFEKERGFESTAQLIEFARDLHREWNNLYEIAVWKESISVKGNRLFYIQAR"
  },
  {
    "reference": "MRVLKFGGSSLADAERFLRVSDIVVNNQQQVQVALVLSAPAKVTNLLVALVAQTSKGEDASATLAEIESIFTRLITGLKARYANFADELLMQRLQQELGTIRSKMQGIQLLNQCPDSVQALILSRGEALSIACMEQLLLSRGEKVTMINPVEMLLAEGNFLESHVDIDASRARFAAKPVNEGHIYLMAGFTGGNAKGELVLLGRNGSDYSAAVLAACVNADCCEIWTDVDGVYSCDPRLVPDAVLLKRMSYKEAMELSYFGAKVLHPRTIAPIARFHIPCLIKNTFNPQGEGTLISADHGDDQYPVKGISDLSGISMINVSGPGMKGMVGMAGRMFTAVSRAGVSVVLITQASSEYSISFCIHTYDAEKALKVLNQEFELELQANLLEEIEIRHDLAILSLVGDGMRTMKGIAARFFTSLAQAAINIVAISQGSSERSISAVVGNKKVTEAIKACHQNLFGTQQFIDIILVGLGGVGGALLQQIRRQQAVLSKQGIGLRVVGLANSRKMALSKDGLDLNNWQEALEKATERFNLQAIKQLVDDSHLINPVIVDCTSDETISGQYADFLAAGFHVVTPNKKANTSSIQYYRELRRTAQATRRKFLYETNVGAGLPVIENLQNLIKAGDKLQAFNGILSGSLSFIFGKLDEGCSFSEATLTAKGNGFTEPDPRDDLSGMDVARKLLILAREAGMPLELSDVIVEQALPPGFDATGSTDEFIKRLPEADAWFSERVAAAKAEGKVLRYVGSIENGQCKVAIKEVDENDPLY",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should interact with enzymes and substrates within the L-threonine from L-aspartate: step 3/5, L-threonine biosynthesis pathway to enhance or regulate its activity.2. The presence of ACT domains is critical for the interaction of the designed protein with its intended targets.3. The designed protein must be able to perform aspartate kinase activity, homoserine dehydrogenase activity under specific conditions. The designed protein sequence is ",
    "response#1": "LLHDKLINKGQELAVIIAGLGNVGEVFIEQLSQQLNRLEQHFSVKLVGLLRSQQMLFSPSGISLEQWQTQWDNEAIDYQQGDLLTHIGELDYEHKVVIDITASESFSLLYPDFVNLDCHLISANKYAGTANNDWYQALRHNLAERNLLWRYNTSVGAGLPVNFALADLQNSGDKITRIEGVFSGTLSWLCSSYDGSVPFSDLVLQAQQMGFTEPDPREDLSGRDMQRKLLILARELGLTLELDDIDLTALMPEQLAQGDWQSFLDNKQQLDNFIADKAKQASEQGKVLRYTGALSLADSKVTAQVGLAFATQGDALANLTPGDNIFVINSQWYSDNALVIQGPGAGKEVTAAQ",
    "response#2": "MRVLKFGGSSLASGQRFIEVASIIKNKSQSSRLAIVVSAPQGVTNHLVAMAENISNEEKLKSDLLHFKHAIENIINDLSATLPGFCQQHSEQVLATWEHQLSRYLHGATLLSFCPEHVRARIISIGERLSVALLQSVLSFDDIAVSVIEPERFLRTNDVAMNAVADLVLCKERFQHVYNELNSIALMPGFIGVDSLGNVTTLGRNGSDYSAAVLAVCSEAEYCEIWTDVDGVYNADPRLIKEAQLLDYLSYQEAMELSYFGASVLHPKTIGPIAQHHIPCLIKNTGNPDAPGTLIANENDQQKRVKAISNLDDLTMVNVSGPGMKGMVGMASRVFATMSRENISLILISQSSSEYCISFCIYSSDAGKAEQSLQEEFELELLNGLLEPLTLTHELSIVSLVGDGMHHQQGVAAKFFSSLAQARVNVVAIAQDSSERSISVVIESRKCTDAMKVSHQNFFSHKPTIDVFLVGCGVVGSELLAQIARQQAKLTADNISLKVYGLANSKGMVFNSEGIDVANWQENLDQEALPVNVENIKNFVRANHLINPVLVDCTSNEALALQYVNYLEAGFNVVTPNKKANTDSWAYYQALRTAAQKTNRRFLYETTVGAGLPVIDTLQNLLKAGDELVRFEGVLSGSLSYIFGKLDEGMTLSQATTLAKEQGFTEPDPRDDLSGMDVARKLLIMAREAGMPLELTDIEIEPVLADSFDASGDVATFMSNLPKLDKAFAERITAAKAEGKVLRYIGNIVDGKCQVAIEAVGVDHPLI",
    "response#3": "MRVLKFGGSSLADADRFLRAADIIANNAKQEEVAVVLSAPGKTTNKLVAIIESAMRHGEAELQVEQLETAFHTLFNDIKAVLPNIEGNEFDKQVRQSMSKLREFVHGITLLSMCPDNVNARIISKGERVSIQLMKAVMEAKGLKADLIDPVKYLSAQGEYLEAMVDVEASTNNFRQHPLADDHVHIMPGFTAGNKKGELVCLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYNCDPRLVKDARLLKSLSYQEAMELSYFGASVMHPKTIAPIAQFQIPCLIKNSFNPQGAGTLIGQDTGEDELEIKGITTLSNLTMVNVSGPGMKGMVGMASRVFGTMSSHGVSIVLITQSSSEYSISFCIEADNKAQAQLALSDEFELELKDGLLEPVEFMDDLAIISLVGDGMRTSCGIASQFFTSLAEVHVNIIAIAQGSSERAISAVIPEGKISEAIKTCHENFFNSKHYLDVFVVGVGGVGGELVDQIHRQQEKLATKGTILRVCGLANSKGLLLDSEGLPLDNWRDRMDSVTEEFSLARLIALVQRNHIINPVLVDCTSSDEIAKQYADFLAAGFHVVTPNKKANTASMAYYQQLREVARHSRRKLMYETTVGAGLPVIENLQNLIAAGDELEQFNGILSGSLSFIFGKLDEGLTLSQATLLAKEKGFTEPDPRDDLSGMDVARKLLILARESGMKLELSDVKVEQALPPGFDDSGSVAEFIERLPQADEYFTQLFNEASAQGKVLRYVGEIADGQCRVRIAMVDENDPI"
  },
  {
    "reference": "MRLILISHSRLFALSALFLIPTFDSLSAENTLTDSTTLIDRTTQSDSTTQRDSKTRRDPAPSFWQRASNNLSETWQHPQSQDLYVPAITWHNRWTYDRAKTDKYNERPWGAGFGVSRLDSDGDWHALYVMAFKDSFNKWEPIGGYGYEKRWRPLENQDIQFGLGFTAGVTMRDNWHYIPIPVLLPLASVSYQRLSFQATYIPGTYNNGNVFFAWLRWQF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. We need a protein that can perform a 1,2-diacyl-sn-glycero-3-phosphocholine + a lipid IIA = a 2-   acyl-sn-glycero-3-phosphocholine + a lipid IIB with high efficiency. The designed protein sequence is ",
    "response#1": "IVQQLEKAEESIYMQAYGMSDSLITTALINAQMRGVKVKILLDRSNLKQKFSKLYELQQAKIDVGIDTVPGIAHNKVIIIDKKKVITGSFNFTVAADKRNAENVILIEDQQLAESYLQNWFR",
    "response#2": "DRPFYISLVRGQPDKVIAHTSKDQSLLNLERGIASLLQLRLDASQEEELDVSGLCRVSYNVKSSTKVEKTKRDCSLWDLRVNYNPEEALGVTQQAQETVFYELSSEGTLLHAESQENHRLNLAAKPDVGSFVKSSLILQHVSQGSEEVKQLQLGSLDKAIQSLLEWYRVFELESDVDGMISAIKEQTLEDQLKASLTELQSADVGKSSLALAYVKLIPLARITRQEQFEDLLTEHAEVLPQLVDLLGAVQTFDAHNATFGFLYKESETTSEQLDLLEKYLQSLAVATHPDRKIVEHLFGLLEQESIKKHLKLRESVIQTVATLTRQSGLDVEDPLLKEVRSYLLQGLTSKEPTLYIRALQNLQDPATIEALLEHAQTGEAPNLSVAALQALKAFPLGSFNSSHRLQFESIFYQRKRRFDSSARTLALDIILSLRPTQEQV",
    "response#3": "NLYQFGKMIKNKTGKPAMFSYSAYGCYCGWGGQGKPQDASDRCCFVHDCCYTRVNDCSPKMTSYSYSFENRDIICGDDDSCRKAVCECDREAAICLGENVNTYDEKYRFYSSSHCGR"
  },
  {
    "reference": "MSKNQNGVSLTSVQALDQNDPLANFKRRFALPKGVLYLDGNSLGAQPIAAKKAVDDTLDQWRDELIKGWNQGWFDAPERLGNLLAPVIGARRGEVTVTDNITLNIFKLLGYIIGQRQGERKAVLSEGGNFPTDGYIAQGFCRISGAEHRFLPENSDLAEHLDGVAVVVLSQVNYRTGKLRDITATTRLVHAHGAEVIWDLAHSAGALPIDLNGSGARYAVGCTYKYLNGGPGSPAFLYVHSNYQDQGWQPLSGWHGHASPFAFDADYIPANDISHFRTGTHSAIIYAALEASLTLWAEVDMAAVRAKSVAMSELFLACLGDIFHRHGIEDITPASSQRGSQVSLIDTQNGYAIVQALIARGVIGDYREPGLMRFGFTPLYLSFEDVWLAAQHFREVVESGEYRDPQLHVRGAVT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis pathway to avoid interference with native metabolic processes.2. The presence of Aminotransferase class V domains is critical for the interaction of the designed protein with its intended targets.3. For general function, the protein need meet that Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively. The designed protein sequence is ",
    "response#1": "LSEGVADLVGAGKNEVVAMNTLTANLHFMMVSFYQPTKTRFKILMEDHAFPSDHYAVESQVKFHGFNPEDAVLFIKPRDGEEAIRDEDVLKLIEDEGESIALIMLPGVQYYTGQVFDMKSITQAGHNKGCKVGFDLAHATGNIVMSLHEWQVDFACWCTYKYLNSGPC",
    "response#2": "LSEGVADLVGAGKNEVVAMNTLTANLHFMMVSFYQPTKTRFKILMEDHAFPSDHYAVESQVKFHGFNPEDAVLFIKPRDGEEAIRDEDVLKLIEDEGESIALIMLPGVQYYTGQVFDMKSITQAGHNKGCKVGFDLAHATGNIVMSLHEWQVDFACWCTYKYLNSGPA",
    "response#3": "HMAEDCARKSADIVGASPEEIVIMNTLTANLHFMMASFYKPTAKRHKIILEWKPFPSDHYTIESQIQWHGLDPSTSMVQIQPDENFLISTEYILATIDEHAEETALILLPGIQYYSGQLFDMPRITKYAQEKGIVVGWDLAHAAGNVELRLHDWNVDFACWCTYKYMNAGT"
  },
  {
    "reference": "MLKGKKVILGITGSIAAYKAAYLLRLLVKEGAEVQVIITPAGKEFITTVTLSALSGKPVLSEFFENTDGSWHSHVDLGIWADIMVVAPASANTMGKMVNAICDNLLITTYLSAKCPVVVAPAMDLDMFAHPANQRNVDVLRQFGAHIVEPGTGELASGLEGKGRMEEPDGIVERLKEILGSEKKKSLSGKKVLVTAGPTYENIDPVRFIGNYSSGKMGFAIAEELATRGAEVSLIAGPVSLNARNSLINRTDVVSANEMDQVCQEIFPDCDAAVFAAAVSDYAPENKSEDKIKRSSDNISLQLRPNPDVAARAGIIKKKGQITVGFALETNNEEVNAAKKVQTKNFDFIVLNSPNNEGEGFMTDTNRISIIHRDHKKENFPLKSKQEVACDIVNVLEQKLLS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.2. The protein must be able to phosphopantothenate--cysteine ligase activity.3. The protein should be designed to catalyze the following reaction H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "EDRRVVITGGGTREKIDEVRYISNFSSGKMAKAIALSLYLKGADVCYITTKGNDSLPKSIYTIEVDDAQEMLEYTQDALRIAKKGKLSKASLTTADAIKLIQKRPFLFMVAAVADFTPKFPQQGKLKKSTLGENWQIELTQTSDILSTLAKDREGVTTIAFKAEMDTQEGLNNAKELLTKKEVDAVCYNLLEDAKSFGGDQNSITFLVEKGE",
    "response#2": "KRILLGVSGGIAAYKSVTLASRLTQANALVDVIMTESATKFIAPVTFQAITQRPVSTDIFDSWSDTGRLRIGHVALAHAADLFIIAPATAQTLARLALGMADDLLSITTLSYKGPMLIAPAMETNMWHHPATQEHVAKLRGWGVDFVGPAEGRLASGEYGAGRVVEPEEVFAFAREAL",
    "response#3": "EDRRVVITGGGTREKIDEVRYISNFSSGKMAKAIALSLYLKGADVCYITTKGNDSLPKSIYTIEVDDAQEMLEYTQDALRIAKKGKLSKASLTTADAIKLIQKRPFLFMVAAVADFTPKFPQQGKLKKSTLGENWQIELTQTSDILSTLAKDREGVTTIAFKAEMDTQEGLNNAKELLTKKEVDAVCYNLLEDAKSFGGDQNSITFL"
  },
  {
    "reference": "MSVPAEVVEKARKLREEIEFHNVRYYRLDDPLITDAEYDRLMAELLAIEARYPELVTPDSPTQRVGAPPVEAFTEVRHEVPMLSLENAFSEEDLTAFDRRVRKELSRDTLEYVAEPKLDGLAVNLLYQDGVLVRAATRGDGEVGEDVTHNVRAVRAIPLRLKGEDFPDRFEARGEVFMPKRGFLALNERARRSGEKTFVNPRNAAAGSLRQLDARVTASRPLSFYAYGIGAFPDARLPATQHELLESLQRWGLPVSPEYRVVRGAAGCLEYYRSLGERRHDLPYDIDGVVYKVDDLKAQAGLGFVSRAPRWAIAHKFPAEEARTRVVGIDVQVGRTGVLTPVARLEPVFVGGVTVTNATLHNADEIRRKDVRPGDTVIVRRAGDVIPEIVRVVPELRKPGAEPFRMPEVCPECGSAVEAEPGEVLARCSGGLYCPAQHKESIKHFASRRAMDIEGLGDKLVDQLVGRKLIATVADLYRLDLDRLAGLDRMGEKSAANLLAALERSKRTTLARFLYALGIRDVGEVTAKVLSEHFGSLEALMEATEEELLAVADVGPVVSRHIRLFFAQPHNREVISQLLACGVSWEKPAPAPRKLPLQGLVFVLTGTLASMSRDEARARLEALGAKCTGSVSRNTSFVVAGSEPGSKLDKASELGVPVLDEKGFLDLLERGRP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double- stranded DNA using NAD as a coenzyme and as the energy source for the reaction.2. The designed protein should have a high specificity for Zn(2+), NAD(+) binding, with minimal off-target effects.3. The designed protein have BRCT domains with specific sequence motifs that are crucial for its activity.4. The designed protein must possess DNA binding, metal ion binding to support DNA replication under certain conditions.5. The protein's N6-AMP-lysine intermediate must be carefully engineered to ensure that the amino acids involved in NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-   (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-   nicotinamide D-nucleotide. are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "MSKKRHEELKKIISEHDHNYHVLDKPTITDYEYDQLFAELLDIEKNPKGLDLSDSPSQRVGGTVLEGFTKAQHRLPMLSLANSYSPEDIFEFDERVRKFLNTEDPVEYLCELKFDGLSMELIYENGQLVRAITRGDGTVGEDVTHNIKTIKSIPLKLSHKNPPPLLEVRGEVLMFKEDFARLNETQQENGQQTFANPRNAAAGTVRQLDSRIAASRPLRFFGYALGAVEGETFNTQKNIQEYFNDHGIPTVLPYKEDLLVVAKGPEEVVKYYHHIEKVRPKLPFDIDGVVIKVNSLRLQEDLGLVARSPRWATAAKFKPEQAQTTVEDIVVQVGRTGALTPVAIMKPVKVGGVTVTNATLHNQDEITRKDIRIGDTVIIQRAGDVIPEVVEVVNPDKRPADRLPYSIPERCPACDSVAVKAEGEVVTRCVNPLCIAVVKESLKHFVARRAMDVDGLGEKLIDQLVIGLGLKQAMDIKHMGEKIVIQLFQEIDQQKNRPLWRLLIGLGIRHVGEKAAKIVSEQFGSMEAVMAATEEQLVAIYEIGDKMASSLVEYFSNDDARAVITALRNAGVKLEEQTTVEDIVVQVGRTGALTPVAIMKPVKVGGVTVTNATLHNQDEITRKDIRIGDTVIIQRAGDVIPEVVEVVNPDSPLLGQTWVLTGTLTSLGRTEAKAKLEALGAKVAGSVSKNTHCVVAGEAAGSKLAKAQELGIPVLDEAGLIELL",
    "response#2": "MSKKRHEELKKIISEHDHNYHVLDKPTITDYEYDQLFAELLDIEKNPKGLDLSDSPSQRVGGTVLEGFTKAQHRLPMLSLANSYSPEDIFEFDERVRKFLNTEDPVEYLCELKFDGLSMELIYENGQLVRAITRGDGTVGEDVTHNIKTIKSIPLKLSHKNPPPLLEVRGEVLMFKEDFARLNETQQENGQQTFANPRNAAAGTVRQLDSRIAASRPLRFFGYALGAVEGETFNTQKNIQEYFNDHGIPTVLPYKEDLLVVAKGPEEVVKYYHHIEKVRPKLPFDIDGVVIKVNSLRLQEDLGLVARSPRWATAAKFKPEQAQTTVEDIVVQVGRTGALTPVAIMKPVKVGGVTVTNATLHNQDEITRKDIRIGDTVIIQRAGDVIPEVVEVVNPDKRPADRLPYSIPERCPACDSVAVKAEGEVVTRCVNPLCIAVVKESLKHFVARRSMDLEGMGERQVELFLRLGLVKTVADIYRLDEALEAVEDVGPVVARHIHTAVADSKSRPWERLLIGLGIRHVGEKAAKIVSEQFGSMEAVMAATEEQLVAIYEIGDKMASSLVEYFSNDDARAVIETVLEGIDIQVGRTGALTPVARLAPITVGGVVITNASLHNEDYIKGIGSKGEPIREGNDIRVGDTVIVQRAGDVIPQIIDIVAEKRSKETVLEGIDIQVGRTGALTPVARLAPITVGGVVITNASLHNEDYIKGIGSKGEPIREGNDIRVGDTVIVQRAGDVIPQIIDIVAEKRSKPAQPLLGQTWVLTGTLTSLGRTEAKAKLEALGAKVAGSVSKNTHCVVAGEAAGSKLAKAQELGIPVLDEAGLIELI",
    "response#3": "MSKKRHEELKKIISEHDHNYHVLDKPTITDYEYDQLFAELLDIEKNPKGLDLSDSPSQRVGGTVLEGFTKAQHRLPMLSLANSYSPEDIFEFDERVRKFLNTEDPVEYLCELKFDGLSMELIYENGQLVRAITRGDGTVGEDVTHNIKTIKSIPLKLSHKNPPPLLEVRGEVLMFKEDFARLNETQQENGQQTFANPRNAAAGTVRQLDSRIAASRPLRFFGYALGAVEGETFNTQKNIQEYFNDHGIPTVLPYKEDLLVVAKGPEEVVKYYHHIEKVRPKLPFDIDGVVIKVNSLRLQEDLGLVARSPRWATAAKFKPEQAQTTVEDIVVQVGRTGALTPVAIMKPVKVGGVTVTNATLHNQDEITRKDIRIGDTVIIQRAGDVIPEVVEVVNPDKRPADRLPYSIPERCPACDSVAVKAEGEVVTRCVNPLCIAVVKESLKHFVARRAMDIDGLGEKLVDQLVIDGLVKSPADLYKLTASKLTMLERMGTKSATNLVAAIDKSKKTTLAKFIFSLGIKNIGEKTAYMLAQQFKSFANWFDKMSMLKDDTQTEDEIRNLDGMGSCICESLLDFFSDSDNCNMIADLRQKGLHLQTTQGVLAGKTVVLTGTLPTLTREAAKEMLEAAGAKVAGSVSKKTDYVVAGADAGSKLAKAEELGIPVLDEAGWLALHKE"
  },
  {
    "reference": "MVTLYSSPSCTSCRKAKLWLEENHIPYTERNIFSDPLTIEEIKEILRMTESGTDEIISTRSKVFQELNVNLESLPLQDLYKMIRDYPGILRRPIMIDEKRLQVGYNEDEIRRFLPRTVRTFQLREAQRLVN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be expressed specifically in the cytosol2. The protein must exhibit the following characteristics: This interaction can enhance binding of RNAP to the promoter region of target genes and stimulate their transcription, or block interaction of RNAP with activator.; Acts by interacting with the C-terminal domain of the alpha subunit of the RNA polymerase (RNAP); Global transcriptional regulator that plays a key role in stress response and exerts either positive or negative regulation of genes. The designed protein sequence is ",
    "response#1": "VTFEDVAVDFTQEEWQQLNPAQKTLHRDVMLETYNHLVSVGCSGIKPDVIFKLEHGKDPWL",
    "response#2": "VTFEDVAVDFTQEEWQQLNPAQKTLHRDVMLETYNHLVSVGCSGIKPDVIFKLEHGKDPWIIESELSRWIYK",
    "response#3": "VTFEDVAVDFTQEEWQQLNPAQKTLHRDVMLETYNHLVSVGCSGIKPDVIFKLEHGKDPWIIESELSRWIYS"
  },
  {
    "reference": "MMQLSNDSHCNKHSLLNVMNRFIAAANNMDETIMVPNLLRDVPLEDQESHASVSHNNNNNNEPSFPNKQRDMYEHYLLLKSIKNDMEWGLLKREMAGGASFLEMAVKQEELPQMKGEAVEEGPDLEGQFHYHLHGLFSVLSKLTVQADHLTNRYKREIGGGSLLR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that May play a role in the regulation of lipogenesis .; Involved in stabilization of microtubules2. The protein should be designed to localize specifically to nucleus, cytosol, microtubule in order to perform its role in regulation of ribonucleoprotein complex localization, regulation of cytoskeleton organization, regulation of lipid biosynthetic process more effectively.3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "QKEREDKLAQILKDRLNEYVINKDEFISNAEAEVARLSNAAYGVDMLNTIGYIYVRQAAKELGKKAIYLGVPFIAEWFRNKGHFIKSQLTAATGAYALFQLQEEMKRQLNTEGNYTEEELEEYLQAHKRVMIDSLWKLNVADIEATLCRVCQLVLQDPEAKREELRTRARGLKALGRIFQRAKTASESE",
    "response#2": "QKEREDKLAQILKDRLNEYVINKDEFISNAEAEVARLSNAAYGVDMLNTIGYIYVRQAAKELGKKAIYLGVPFIAEWFRNKGHFIKSQLTAATGAYALFQLQEEMKRQLNTEGNYTEEELEEYLQAHKRVMIDSLWKLNVADIEATLCRVCQLVLQDPEAKREELRTRARGLKALGRIFQRAKTASESS",
    "response#3": "QKEREDKLAQILKDRLNEYVINKDEFISNAEAEVARLSNAAYGVDMLNTIGYIYVRQAAKELGKKAIYLGVPFIAEWFRNKGHFIKSQLTAATGAYALFQLQEEMKRQLNTEGNYTEEELEEYLQAHKRVMIDSLWKLNVADIEATLCRVCQLVLQDPEAKREELRTRARGLKALGRIFQRAKTASESG"
  },
  {
    "reference": "MFGNCQKQNCCTQRNNTGICYSVCPPQTVITVPGNVTCNASRIYVNIGRPNNCFGNDGDLYLDTNTNNLYYKRDGVWLLVGNLSGSSGPSGPQGPKGEKGSNGDKGDKGEIGIQGLKGESGADADKGDKGDKGDKGSKGTKGENGDKGNKGDKGDPGIKGSKGEKGSKGDKGSKGDKGDPGIKGESGADADKGDKGDKGSKGDKGDKGIDGNKGEKGSKGDKGDKGDIGLKGESGADADKGDKGDKGSKGDKGDKGDIGPKGESGADADKGDKGDKGSKGDKGDKGTKGESGLIGTKGDKGDKGDKGIKGDKGEAGTSILEGSGVPSPDLGNNGDLYIDGMTGLLYAKINDEWVPVTSIKGDKGDKGDTGLKGESGADADKGEKGDPGNKGDKGNKGDKGSKGDKGDKGDKGDTGLKGESGADADKGDKGGKGEKGDNGEKGSKGEKGEKGEKGDNGEKGDKGDNGEKGEKGEKGEKGDNGEKGEKGDVGIKGESGADADKGDKGEKGDKGVNGDKGDKGSKGDTGIKGEAGTAANKGDKGSKGDKGDKGSKGDIGISIKGDKGDKGDKGSKGDKGDIGIKGESGLSIKGDKGDKGGKGDKGDLGSKGDIGLKGDKGDKGDVGLKGDKGDKGDVGSKGDKGDKGSKGDKGSKGDTGSKGDKGDKGSKGDKGDKGDKGSKGDKGDIGSIGPKGEKGEAGSTNSLYIGSAQLGNAGDFNNEIIPVTGIAYITAVGAGGSGYSGTTGGYGGGGAGGAFINYPVYVSSSQTY",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The presence of Collagen-like domains is critical for the interaction of the designed protein with its intended targets.2. The designed protein should be expressed specifically in the virion component3. The designed protein's functionality will benefit from Basic and acidic residues, Polar residues compositional bias in its structure.4. The protein must exhibit the following characteristics: May participate in the formation of a layer of cross-linked glycosylated fibrils at the viral surface thus giving it a hairy-like appearance.. The designed protein sequence is ",
    "response#1": "SQDMPTNTTDITYVGDNATYSVPMVTSEDANSPNVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASNRTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTSPTLNTTGFADPE",
    "response#2": "SQDMPTNTTDITYVGDNATYSVPMVTSEDANSPNVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASNRTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTSPTLNTTGFADPV",
    "response#3": "SQDMPTNTTDITYVGDNATYSVPMVTSEDANSPNVTVTAFWAWPNNTETDFKCKWTLTSGTPSGCENISGAFASNRTFDITVSGLGTAPKTLIITRTATNATTTTHKVIFSKAPESTTTSPTLNTTGFADPA"
  },
  {
    "reference": "MKNVWLALVGIVMAFSVTAAQFTDGKQYLTLDKPVTGEPQVLEFFSFYCPHCYQFEEVYQVPKAVKKALPEGTKMTRYHVEFLGPLGKQLTQAWAVAMALGVEEKITPLMFEGVQKTQTVQTPGDIRNVFIKAGISGEDYDAALNSFVVKSLVAQQQKAAEDLQLRGVPAMFVNGKYMIKNDGMDTSSMDNYVKQYADVVTFLLTQK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein can be expressed in periplasmic space and perform disulfide oxidoreductase activity without triggering undesirable cellular responses.2. For general function, the protein need meet that Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA; DsbA is reoxidized by DsbB; It is required for pilus biogenesis .; Acts by transferring its disulfide bond to other proteins and is reduced in the process3. The presence of Thioredoxin domains is critical for the interaction of the designed protein with its intended targets.4. The protein must contain a signal peptide for proper functionality. The designed protein sequence is ",
    "response#1": "WWTPLLFLALGVGLAFTPCVLPMYPILTSIVLGGAQLTQRRALLLSVIYVQGMALTYTLLGLVVASAGLQFQAALQHPYVLMGLSVLFVALALSMFGLYSLQLPSGVQTWLNSLSNAQQGGSLPGVFAMGAISGLVCSPCTTAPLSGALLYVAQSGDLLTGAVALYALAIGMGIPLILVAVFGNKLLPKAGNWMERVKTLFGFVLLAAPIFLLERIL",
    "response#2": "LLFFFLAGLTLTFTPCVLPMLPILSGVVLRGRPGGGRGFVLSLAYVLPMALCFALLGALMGMFGASLNLQAQLQSPWVLVPFAAFFALFAVAMFGFFELRLPGFIREPLDRLAGDARGGSILGAATLGVLSSLLVSPCVSAPLAASLLYISASGDAWGGGLQLFALGLGMGTPLVVFGAGGGALLPKSGAWMNGVRNAFGVLLLAVAVWLLERVL",
    "response#3": "LLFFFLAGLTLTFTPCVLPMLPILSGVVLRGRPGGGRGFVLSLAYVLPMALCFALLGALMGMFGASLNLQAQLQSPWVLVPFAAFFALFAVAMFGFFELRLPGFIREPLDRLAGDARGGSILGAATLGVLSSLLVSPCVSAPLAASLLYISASGDAWGGGLQLFALGLGMGTPLVVFGAGGGALLPKSGAWMNGVRNAFGVLLLAVAVWLLERVL"
  },
  {
    "reference": "MCFSRADAADNYPFGTCQQRKLFPHFHPPNLIGNKFVPLRGSPHRGPGCYFSDGYGLAYDLSKIPTSIKGYTLGARTAVRFKPIQKEMTPHAGRYQKVSPQQEKHKQNFAPFNVLVPRFKNYPKDTYYPSPGAYNPEKKPPPKIAWPMKFGSPDWAQVPCLQKRTLKAELSTDKDFRKHRNRVAYLSLYYN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that During primary cilia disassembly, involved in cilia disassembly; May act by stimulating AURKA activity at the basal body in a cell cycle- dependent manner.2. A protein that contributes to cilium organization by functioning in ciliary basal body with kinesin binding, beta-tubulin binding, gamma-tubulin binding. The designed protein sequence is ",
    "response#1": "GWRSPKFCQFPQEIVLQMVERCRVRKLQLLAHQYMISSKVEFYISESLPEYLVPYQAERFRRLGYVSLCDNEKTGCKARELKSVYVDAVGQFLKLIFHQNHANKYNIYNQVALVAINIIR",
    "response#2": "GWRSPKFCQFPQEIVLQMVERCRVRKLQLLAHQYMISSKVEFYISESLPEYLVPYQAERFRRLGYVSLCDNEKTGCKARELKSVYVDAVGQFLKLIFHQNHANKYNIYNQVALVAINIIC",
    "response#3": "RKRPLGVREVRRGEVNVITVEDKETLLVHEKKEAVDLTQYILQHVFYFDEVFGEACSNQDVYLKTAHPLIQHIFNGGSATCFAYGQTGAGKTYTMIGTHQNPGLYALAAKDIFRQLKVSQSRRNLFVWISFYEIYCGQLYDLLNRRKRLFAREDSKHVVQIAGLRELQVDSVELLLQVILKGSKERSTGATGVNADSSRSHAIIQIQIKDSAKRTFGRISFIDLAGSERAADARDSDRQTKMEGAEINQSLLALKECIRALDQEHTHTPFRQSKLTQVLKDSFIGNAKTCMIANISPSHIATEHTLNTLRYADRVKR"
  },
  {
    "reference": "MGDRLNIVVGITGGIAAYKAATVVRLLVKAGHDVHVVPTESALRFVGAPTLEALSRNPVTTSIFDDVAAVRHVALGQAADLVVIAPATANSLAKLAAGMADDLLGTTVLASRAPLVVAPAMHTEMWSNPATVANVATLRSRGVVVVGPASGELTGGDRGPGRLSEPEEIVAAALAAVVPARGDLPGRDLAGHDLPGRDLAGRHVVVTAGGTREPLDPVRFLGNRSSGRQGVALARAAADRGARVTLVAAHLDADVAQQAPGTGITVVEASTTRSLQEAVTAAARDADVVVMAAAVADYRPERVADAKIKKAGDGADLTLRLVENPDVLRGLVETRRPGQIVVGFAAETAESRDELLALGRAKAERKGADLLVLNTVGWSAGFGTADNDVVVLDASGTEVAAARGSKREVAEAVLDEIVGRLLR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Ideal protein should have a binding affinity for key enzymes in the CoA from (R)- pantothenate: step 3/5, coenzyme A biosynthesis, Cofactor biosynthesis pathway to enhance the overall efficiency of the pathway.2. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domain.3. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A4. The protein should have a low Km value for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.5. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.6. A protein with FMN binding, phosphopantothenoylcysteine decarboxylase activity functions is required to enable pantothenate catabolic process, coenzyme A biosynthetic process. The designed protein sequence is ",
    "response#1": "KVVLGVTGGIAAYKTAELARIFIRSGVEVQVVMTEAASRFVNPLTFTTLTGNPVHFQMYDGQSTGIRHIDLSEGADAMIIAPATANTIAKMATGLADNLLTTLFLATLCPVIIVPSMNVKMFEHPAVQNNLQVLRSRGCHILEPNAGELACGEYGRGRMPEPQEIFDVVRVLAGDALLAGVTVAVSAGGTREPIDPVRYLGNRSSGRQGVAVAVAAAERGARVLLAACNVEAEVLRPAEAAGVEVRRAGSAAELSEVMREVAERADAVIMAAAVADYRVDAVSERKLTKEDGEPPVLRLVENEDIVAGLAARRRPGQVVVAFAAETAVGEELLARASRKRRRKGVDLIVVNEVGWGKGFEVAENRLLLVRGAEVVEWPQASKDQVAHKWFDLAGVTVAVSAGGTREPIDPVRYLGNRSSGRQGVAVAVAAAERGARVLLAACNVEAEVLRPAEAAGVEVRRAGSAAELSEVMREVAERADAVIMAAAVADYRVDAVSERKLTKEDGEPPVLRLVENEDIVAGLAARRRPGQVVVAFAAETAVGEELLARASRKRRRKGVDLIVVNEVGWGKGFEVAENRLLLAGRHVLVSAGGTREPLDPVRFLGNRSSGKQGYALAAAAAARGARVTLLSANVSLPGPAGVDVIPVATALELREAAVKAAPEADVVVMAAAVADFRPAVYATGKIKKVEGEEPAPVALVRNPDILAELSADRTRPGQLVVGFAAETDQVLAHGRAKLARKGCDLLVVNEVGDGKAFGTDTNEAVIVRRDGTETAVADGS",
    "response#2": "KVVLGVTGGIAAYKTAELARIFIRSGVEVQVVMTEAASRFVNPLTFTTLTGNPVHFQMYDGQSTGIRHIDLSEGADAMIIAPATANTIAKMATGLADNLLTTLFLATLCPVIIVPSMNVKMFEHPAVQNNLQVLRSRGCHILEPNAGELACGEYGRGRMPEPQEIFDLAAGVLALAGRRVLVTAGPTREAIDPVRFVSNRSSGRMGYALAEVAWRRGAAVTLVSGPTALPPPVGVELVRVETAAAMAEAVAAHLPAADVLVMAAAPADFRPAAPAAAKLTKEDGLPPPLAWALTADILQSTRPLRRPDTRVVGFALETADDALARARAKLEAKGLDLVVLNRADEADAGFDTETNRVTVLWPGGREELPLMSKTQLARALVDRVAALLPPAPAPAA",
    "response#3": "KVVLGVTGGIAAYKTAELARIFIRSGVEVQVVMTEAASRFVNPLTFTTLTGNPVHFQMYDGQSTGIRHIDLSEGADAMIIAPATANTIAKMATGLADNLLTTLFLATLCPVIIVPSMNVKMFEHPAVQNNLQVLRSRGCHILEPNAGELACGEYGRGRMPEPQEIFDALAALLAGREPLAGRRVLVTAGPTREAIDPVRFVSNRSSGRMGYALAEVAWRRGAAVTLVSGPTALPPPVGVELVRVETAAAMAEAVAAHLPAADVLVMAAAPADFRPAAPAAAKLTKEDGLPPPLAWALTADILQSTRPLRRPDTRVVGFALETADDALARARAKLEAKGLDLVVLNRADEADAGFDTETNRVTVLTPSGVEIWPRLSKKEVAIALVDWIAERFPRGS"
  },
  {
    "reference": "MAFIALGINHKTASVEVRERVAFTPEQLVEALQQLCRLTPSREAAILSTCNRSELYLEQDQLSSDEVLKWLADYHRLSLDELRACAYVHSEQDAVRHMMRVACGLDSMVLGEPQILGQLKSAYAVAREAGTVGPLLGRLFQATFSTAKTVRTDTAIGENPVSVAFAAVSLAKQIFADLHRSQALLIGAGETISLVARHLHDQGIKRIVVANRTLERASQLAEQFGAHAVLLSDIPDELAHSDIVISSTASQLPILGKGAVESALKKRKHKPIFMVDIAVPRDIEPQVGELDDVYLYTVDDLHEVIEENLKSRQGAAQAAEELVAAGTEDFMQRLRELAAVDVLKAYRQQAERLRDEELAKAQRMLVNGASAEDVLAQLARGLTNKLLHAPSVRMKKLTAEGRIDALSLAQELFALDEGAPQDKGLQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA)..2. The NADP(+), substrate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MAVAARESSRKLQALSSEDRKQILHDIANALEVNEKTIKAENDLDVAAAQEAGYEESLVARLVMKPGKISSLAASVRQLAEMEDPIGRVLKKTQVADDLILEKTSSPIGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKNSTKIPVLGHADGICHVYVDKSGKLDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNGFLDDEAEGIVRAELELLLRAMKHREVEPLLAEIRGRMESLRQREAGKAIKKIENSKDPERVVEGLTRSIVDKIFHDIALKIRDAAERDDREFLRMCSELFAR",
    "response#2": "MAVAARESSRKLQALSSEDRKQILHDIANALEVNEKTIKAENDLDVAAAQEAGYEESLVARLVMKPGKISSLAASVRQLAEMEDPIGRVLKKTQVADDLILEKTSSPIGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKNSTKIPVLGHADGICHVYVDKSGKLDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNGFLDDEAETIVAQETSEFMAWLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAPTKSLQQAARDGDNERLNILRDSLAL",
    "response#3": "MAVAARESSRKLQALSSEDRKQILHDIANALEVNEKTIKAENDLDVAAAQEAGYEESLVARLVMKPGKISSLAASVRQLAEMEDPIGRVLKKTQVADDLILEKTSSPIGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKNSTKIPVLGHADGICHVYVDKSGKLDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNGFLDDEAETIVAQETSEFMAWLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAPTKSLQQAARDGDNERLNILRDSLAS"
  },
  {
    "reference": "MNSRPSNPTKLVIRSSTLLFCGVVLIHLFAAQIDAQRSTSRWQTLNGDAPLVIARGGFSGLYPDSSIAAYQLATLTSVADVVLWCDLQLTKDGLGICFPDLNLANASTIDRVYPNREKSYSVNGVTTKGWFPNDFSLTELQNFLLIRGILSRTDRFDGNGYLISTIEDVVTTLNREGFWLNVQHDAFYEQQNLSMSSFLLSVSRTVSIDFISSPEVNFFKKITGSFGRNGPTFVFQFLGKEDFEPTTNRTYGSILSNLTFVKTFASGILVPKSYILPLDDEQYLVPHTSLVQDAHKAGLQVYVSGFANDVDIAYNYSSDPVSEYLSFVDNGDFSVDGVLSDFPITASAAVDCFSHIGRNATKQVDFLVISKDGASGDYPGCTDLAYEKAIKDGADVIDCSVQMSSDGVPFCLRSIDLRNSIAALQNTFSNRSTSVPEISSVPGIFTFSLTWPEIQSLTPAISNPFRVYRIFRNPREKNSGKLISLSQFLDLAKTYTSLSGVLISVENAAYLREKQGLDVVQAVLDTLTEAGYSNGTTTTKVMIQSTNSSVLVDFKKQSKYETVYKIEETIGNIRDSAIEDIKKFANAVVINKDSVFPNSDSFLTGQTNVVERLQKSQLPVYVELFRNEFVSQAYDFFSDATVEINAYIYGAGINGTITEFPFTAARYKRNRCLGREEVPPYMLPVNPGGLLNVMSPLSLPPAQAPNQDFIEADVTEPPLSPVIAKAPTSTPGTPSTIAQAPSGQTRLKLSLLLSVFFLSLLLL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must be engineered to have a cooperative binding mechanism with Ca(2+) to ensure proper regulation.2. The designed protein should have glycerophosphodiester phosphodiesterase activity, calcium ion binding within plasma membrane to regulate lipid metabolic process, glycerol metabolic process.3. The protein have enhanced a sn-glycero-3-phosphodiester + H2O = an alcohol + H(+) + sn-   glycerol 3-phosphate.4. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.5. The protein must exhibit the following characteristics: Hydrolyzes glycerolphosphoglycerol, glycerophosphocholine and glycerophosphoethanolamine in vitro..6. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "MQVWGTEHLDLEEPYVIVCNHQASLDLLGMVEVIPERCVPIAKKELLYMGTVGWACWLSGIIFIDRHRREAAIDVISQTARTMWRENLRVWVFPEGTRNQNKSMLPFKRGAFHLAVQAQVPIVPIVIRFDVSGWEHLQTEGPYVIISNHQSSLDVLGLMEVLPDRCTMIAKKELIYAGTVGLVCWLGGIVFINRKKTSDAKSVMAEAAKTMLDDQIRLWVFPEGTRNQKGDLLPFKKGAFHLAVAAQAPIIPIVFSSYNNFYERKEKRFTSGTCTIRVLPEVETKGMTADDVPELTSRCFEVMRSCFL",
    "response#2": "HRGASGYVPEHTILSYETAQKMKADFIELDLQMTKDGKLIVMHDEKLDRTTNGMGWVKDHTLADIKKLDAGSWFNEAYPEKAKPQYVGLKVPTLEEVLDRFGKHANYYIETKSPDTYPGMEEKLIASLQKHKLLGKHSKPGQVIIQSFSKESLVKVHQLQPNLPTVQLLEAKQMASMTDAALEEIKTYAVGAGPDYKALNQENVRMIRSHGLLLHPYTVNNEADMHRLLDWGVTGVFTNYPDYPAIEFYEGLSPLVRQCDYALDWIFLESGRAVYRQDESSDSTYIVLSGRMRSVITHPGGKKEIIGEYGKGDLVGIVEMITETSRTTTVMAVRDSELAKLPEGLFNAIKLRYPIVVTRLISFLAT",
    "response#3": "ALDWIFLESGRAVYRQDESSDSTYIVLSGRMRSVITHPGGKKEIIGEYGKGDLVGIVEMITETSRTTTVMAVRDSELAKLPEGLFNAIKLRYPIVVTRLY"
  },
  {
    "reference": "MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYEQVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYTNDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQKIRQEYKNKQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein have enhanced [thioredoxin]-disulfide + H2O + L-methionine = [thioredoxin]-   dithiol + L-methionine (S)-S-oxide.2. For general function, the protein need meet that Has an important function as a repair enzyme for proteins that have been inactivated by oxidation The designed protein sequence is ",
    "response#1": "MEIAIFAMGCFWGVERLFWQLHGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYDPSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAAMLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIG",
    "response#2": "MEIAIFAMGCFWGVERLFWQLHGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYDPSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAAMLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIH",
    "response#3": "MEIAIFAMGCFWGVERLFWQLHGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYDPSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAAMLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIG"
  },
  {
    "reference": "MDSKVTTYAVLGSTGNCGSALIRNLLGKPQSRVHAFCRNKKKLLRLIPEIADSKQVEVFEGGMQDHDLLAACIKNTRAVFMCISTNDNVPGCRLSQDSAIALVKVMRELGYDKPDGGPNGPAPKLLLLSSGTIDPHLSKHLPSLLLWILKRSASHVYADLEKAEEFLRAQESWLTTIYIKPGALSVDKKRGYELSLTEQKDPVSYLDLSAGMIEAADDPEGRWDLKNISVNCGSTPAGFPDGTILCILMGLLRHYFPWTHAYLPMGMGPK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. Ideal protein should have a binding affinity for key enzymes in the Pigment biosynthesis pathway to enhance the overall efficiency of the pathway.2. The protein must be able to monooxygenase activity.3. The protein must exhibit the following characteristics: Oxidoreductase; part of the gene cluster that mediates the biosynthesis of the bianthraquinone cladofulvin, a conidial pigment not required for virulence but that plays a role in fitness and resistance to environmental stresses including UV light and low-temperature stress; The pathway begins with the synthesis of atrochrysone thioester by the polyketide synthase (PKS) claG; This compound is decarboxylated by claH to yield emodin, which is further converted to chrysophanol hydroquinone by the reductase claC and the dehydratase claB; The cytochrome monooxygenase P450 claM then catalyzes the dimerization of nataloe-emodin to cladofulvin.; The atrochrysone carboxyl ACP thioesterase claF then breaks the thioester bond and releases the atrochrysone carboxylic acid from claG. The designed protein sequence is ",
    "response#1": "QSKLAIVGMSCRLPGGATSNELFWDVLRRGVDTSQVIPADRFDVATHYDPAGKQLNKSMTQYGCFIDEPGLFDAPFFNMSPREAQTVDPQMRLALVTAYEALEQAGYVANRTASTRLERIGTYYGQAADDYREVNQGQEVSTYYIPGGCRAFGPGRINYFFKFAGPSYSIDTACSSGLAAVEVACQALWRGDVDTAVTGGVNILTNPDGFTGLCSGHFLSKGHNACKTWDATADGYCRADGVGSLVIKRLEDAEDDNDNILGVILGAGTNHSAQAVSITHPHAGHQAYLARQVLRQAGVDPLDVSYVELHGTGTQAGDSEEMQGILDVYAPLSKRRSQSQPLHIGAVKANMGHSESSAGTTALVKVLLMLQNNVIPPHIGITTEMNPKFGHDFKKRNLHIPFELTPWEHTDDKRRIAVVNNFGAAGGNTTMILEGYPDPPEDIDTSTVQQIVEQVFDTDTGSCTVVIESDMMQPDFLAAAHGHRMNDCGVATSSIHGDIAFTLAEHIHKKLGVHGKDTRYNVANLQVTKALVARKNTANPQVIRVTASTTDVRSGIDLVWQNINANGDSAEPFATSSIHAGISTDWISSWSSLTHLVRDRIETLDRLVAEGKANRLSHNMAYTLFASNLVDYADKYRGMQSVVMHGLEGCADVELSTKESGVWTVPPYFIDSVAHLAGFIMNCSDAIDAKKFFCITPGWKTMRFARPLVPGARYQSYVKMIPTVEDDTAYFGDVYILQDGVIIGLVEGIEFHRYRRILLERLF",
    "response#2": "LAIVGMSCRLPGGATSNELFWDVLRRGVDTSQVIPADRFDVATHYDPAGKQLNKSMTQYGCFIDEPGLFDAPFFNMSPREAQTVDPQMRLALVTAYEALEQAGYVANRTASTRLERIGTYYGQAADDYREVNQGQEVSTYYIPGGCRAFGPGRINYFFKFAGPSYSIDTACSSGLAAVEVACQALWRGDVDTAVTGGVNILTNPDGFTGLCSGHFLSKGHNACKTWDATADGYCRADGVGSLVIKRLEDAEDDNDNILGVILGAGTNHSAQAVSITHPHAGHQAYLARQVLRQAGVDPLDVSYVELHGTGTQAGDSEEMQGILDVYAPLSKRRSQSQPLHIGAVKANMGHSESSAGTTALVKVLLMLQNNVIPPHIGITTEMNPKFGHDFKKRNLHIPFELTPWEHTDDKRRIAVVNNFGAAGGNTTMILEDAPQQIVEQVFDTDTGSCTVVIESDMMQPDFLAAAHGHRMNDCGVATSSIHGDIAFTLAEHIHKKLGVHGKDTRYNVANLQVTKALVARKNTANPQVIRVTASTTDVRSGIDLVWQNINANGDSAEPFATSSIHAGISTDWISSWSSLTHLVRDRIETLDRLVAEGKANRLSHNMAYTLFASNLVDYADKYRGMQSVVMHGLEGCADVELSTKESGVWTVPPYFIDSVAHLAGFIMNCSDAIDAKKFFCITPGWKTMRFARPLVPGARYQSYVKMIPTVEDDTAYFGDVYILQDGVIIGLVEGIEFHRYRRILL",
    "response#3": "TSTVQQIVEQVFDTDTGSCTVVIESDMMQPDFLAAAHGHRMNDCGVATSSIHGDIAFTLAEHIHKKLGVHGKDTRYNVANLQVTKALVARKNTANPQVIRVTASTTDVRSGIDLVWQNINANGDSAEPFATSSIHAGISTDWISSWSSLTHLVRDRIETLDRLVAEGKANRLSHNMAYTLFASNLVDYADKYRGMQSVVMHGLEGCADVELSTKESGVWTVPPYFIDSVAHLAGFIMNCSDAIDAKKFFCITPGWKTMRFARPLVPGARYQSYVKMIPTVEDDTAYFGDVYILQDGVIIGLVEGIEFHRYRRILLERLFKPGMSEEDYRNHMVNVSAPLTKDLMVKYGVKRWTQIHNQSETKALMSQLFDPQMCNLADYDCFSQVVFENIEDYKRMKQDPWYKKHLVGDHENFADQQIVEQVFDTDTGSCTVVIESDMMQPDFLAAAHGHRMNDCGVATSSIHGDIAFTLAEHIHKKLGVHGKDTRYNVANLQVTKALVARKNTANPQVIRVTASTTDVRSGIDLVWQNINANGDSAEPFATSSIHAGISTDWISSWSSLTHLVRDRIETLDRLVAEGKANRLSHNMAYTLFASNLVDYADKYRGMQSVVMHGLEGCADVELSTKESGVWTVPPYFIDSVAHLAGFIMNCSDAIDAKKFFCITPGWKTMRFARPLVPGARYQSYVKMIPTVEDDTAYFGDVYILQDGVIIGLVEGIEFHRYRRILLE"
  },
  {
    "reference": "MAAMTVHLDVVSAEQSLFSGRVESIQVTGSEGELGVNAGHAPLLTALKPGMVRLVKQFGEEEFIYIAGGTLEVQPNLVTVLADTAVRGEDLDEQAAEAAKRDAEAQMAKGASAELDYNQAAVQLAEAIAQLRIIQQLRKK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that localizes to proton-transporting ATP synthase complex, catalytic core F(1), plasma membrane and can perform ATP binding with high specificity and efficiency.2. The protein must exhibit the following characteristics: Produces ATP from ADP in the presence of a proton gradient across the membrane.. The designed protein sequence is ",
    "response#1": "MKSVAGRLRLDLAQYRELEAFSAFGSDLDKASRDQLARGARLVELLKQPQGQPFPVERQVVSIWAGTTGKLDDVPVADIRRFESEFLDFVGRSYPGVYDAIVTTGKLSDDTIAMLESAVAEFKKQL",
    "response#2": "GIKAIDAMTAIGRGQRQLIIGDRQTGKTTVAIDAIINQRDNWTSGDPSKQVKCVYVAIGQKKSTIREVVNSLEEAGALAYTTIVAAPADEPAGFKYIAPYTGSAIAQHWMYNGQHALIVFDDLTKQAEAYRAISLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDELGGGSLTGLPIIETKANDISAYIPTNVISITDGQVFLESDLFNQGVRPAINVGTSVT",
    "response#3": "MKSVAGRLRLDLAQYRELEAFSAFGSDLDKASRDQLARGARLVELLKQPQGQPFPVERQVVSIWAGTTGKLDDVPVADIRRFESEFLDFVGRSYPGVYDAIVTTGKLSDDTIAMLESAVAEFKKQL"
  },
  {
    "reference": "MTEKELIESGNMKMEWARNHMPVLAIIREKFEKEKPLKGLKVGMALHVEAKTAVLVETLAAGGAQVAISGCNPLSTQDDVAAALDTRENINCFAKYGCCTGEYYEAIDRVLDIEPDITIDDGADLIFKLHKERQEMLAKILGGCEETTTGVHRLHAMEKDGALKMPVIAVNDAMTKYLFDNRYGTGQSAWDGINRTTNLLVAGKNVVVAGYGWCGRGVAMRATGLGASVIVTEIDPIRALEARMDGHRVMKMSEAAKIGDLFVTATGNRDILTADNFKVMKDGAILANSGHFNVEIDMEALDSLAKSTRTVRHNIKEYDIGNRRINVIAEGRLVNLAAGDGHPAEVMDMSFANQALCVRYIAENKLSSGVHGVPRELDTYVAKLKLESMGIATDELTSKQECYMSGWECGT",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be able to perform trialkylsulfonium hydrolase activity under specific conditions.2. The protein must exhibit the following characteristics: Catalyzes the hydrolysis of S-inosyl-L-homocysteine (SIH) to L-homocysteine (Hcy) and inosine; Likely functions in a S-adenosyl-L- methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions; Can also catalyze the reverse reaction in vitro, i.e. The designed protein sequence is ",
    "response#1": "PTTTIGSFPQTTEVRSWRAKFKKGELTTEQYNDLIEKETEAAIRFQEETGIDVLVHGEFERNDMVEYFGEQLDGFTFTKNGWVQSYGSRCVKPPVIYGDVSRPNPMTVKWSKYAQSLTSKWVKGMLTGPVTILQWSFVRNDQPRSETCTQIALAVRDEVVDLEKAGIKIIQIDEPAIREGLPLRKEEWAKYLDWAVKAFRISASGVNDDTQIHPHMCYSEFNDIIQNIADMDADVITIECSRSQMELLDAFANFKYPNEIGPGVYDIHSPRVPSSKEMVRLLEKASAVIPVDQLWVNPDCGLKTRHWNETKKALIEMVAAAQRVS",
    "response#2": "FPTTTIGSFPQTKDIRINRNKFNKGTISAEEYEKFINSEIEKVIRFQEEIGLDVLVHGEPERNDMVQYFGEQINGYAFTVNGWVQSYGSRYVRPPIIVGDLSRPKAMSVKESVYAQSITSKPVKGMLTGPITCLRWSFPRDDVDQKTQAMQLALALRDEVNDLEAAGIKVIQVDEPALREGLPLREGTERSAYYTWAAEAFRVATSGVANKTQIHSHFCYSDLDPNHIKALDADVVSIEFSKKDDANYIAEFKNYPNHIGLGLFDIHSPRIPSKDEFIAKISTILKSYPAEKFWVNPDCGLKTRGWEETRLSLTHMVEAAR",
    "response#3": "LPTTTIGSYPQTTQIRVARAARRKGEIDEAEYLKRMRAEVADVVALQEKLDLDVLVHGEPERNDMVQYFAEQLDGFFATDNGWVQSYGSRCVRPPILYGDVRRSNPMTVEWITYAQSLTQRPVKGMLTGPVTILAWSFVRDDQPLADSANQVALAIRDETVDLQGAGIRIVQVDEPALRELLPLRAADQPGYLDWSVGAFRLATSGVSDSTQIHTHLCYSEFGEVIEAIARLDADVTSIEAARSHMEVLDDLSAVGFDLGVGPGVYDIHSPRVPGVEEIAASLREALKAVPVERLWVNPDCGLKTRGPAEVEASLRNLVDAAKSVSK"
  },
  {
    "reference": "MFDRLQAVEDRYERLTELLSDPDIVNDSKKLREYSKEQSDIQETVDTYREYKNVKEQLVDTREMLDSEKDPDMHEMVKEEFNLLKAQQEELEERLRILLIPKDPNDNKNVIMEIRGAAGGDEANIFAGDLFRMYSRYAETQGWKIDIMEATPNPMGGYKEVIFMINGQGAYSKFKFENGAHRVQRVPATESQGRIHTSTATVACLPEVEEVDVEIHEKDIRVDTFASSGAGGQSVNTTMSAVRMTHLPTGVVVSMQDERSQIKNREKAMKILRARVADMYMQEAQKEIDATRKSAVGSGDRSERIRTYNYPQNRVTDHRIGLTIQKLDQIVEGRLDEIIDTLILEEQASKLERLNDDL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA. The designed protein sequence is ",
    "response#1": "VIREKKLVNEFLKHVVKDTGLATYGEREVRRALEIGAVDVLLISEGYNKVRVRVKCNHCGWEELKTMSEEEYEVYRKKITKCPKCGSQNLTIEKWDVAEELIKMAEEAGSDVEIISLDTEEGQQFYRAFGGLGAILRFKP",
    "response#2": "VIREKKLVNEFLKHVVKDTGLATYGEREVRRALEIGAVDVLLISEGYNKVRVRVKCNHCGWEELKTMSEEEYEVYRKKITKCPKCGSQNLTIEKWDVAEELIKMAEEAGSDVEIISLDTEEGQQFYRAFGGLGAILRFKP",
    "response#3": "VIREKKLVNEFLKHVVKDTGLATYGEREVRRALEIGAVDVLLISEGYNKVRVRVKCNHCGWEELKTMSEEEYEVYRKKITKCPKCGSQNLTIEKWDVAEELIKMAEEAGSDVEIISLDTEEGQQFYRAFGGLGAILRFKF"
  },
  {
    "reference": "MNAIILAAGFGSRLMPLTKDQPKCMVEYKNKKIIDYEIEALKSAGINEIAVVGGYLNDVLKNYLNKYDIEHFFINSKYDKTNMVHTFFCAKDFMLKCIEEKQDLIISYADIVYFQDCVQKLINAKEELAIVVDKSWCKLWSKRFANPLEDAETLKMTNGYIIELGKKANAYDEIEAQYIGLFKFSYQFLSEVIAFYEMLDRDILYDNKNFENMYMTSFLQALIEKYNNAKAVEIDGNWCEIDFMSDLEVQIEK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be optimized for cytidylyltransferase activity activity in order to improve capsule polysaccharide biosynthetic process. The designed protein sequence is ",
    "response#1": "DADTTIGGVFRFVGDKNPFAWIDFAARYLQHHPATRFVLVGDGDLRAEAQKRAEQLGILERILFVGASRDVGYWLQKMNVFILFSRYEGLPNVLIEAQMVGVPVISTPAGGSAECFIEGVSGFILDDAQTVA",
    "response#2": "DADTTIGGVFRFVGDKNPFAWIDFAARYLQHHPATRFVLVGDGDLRAEAQKRAEQLGILERILFVGASRDVGYWLQKMNVFILFSRYEGLPNVLIEAQMVGVPVISTPAGGSAECFIEGVSGFILDDAQTVR",
    "response#3": "HGYVASPGSRAFFGSSAGGNLNTNVGRAQWEPQSIEAPKNTFITGKLASAGVSGFEPLDEQTATRWHKTNITTGPLDITWNLTAQHRTASWDYYITKNGWNPNQPLDIKNFDKIASIDGKQEVPNKVVKQTINIPTDRKGYHVIYAVWGIGDTVNAFYQAIDC"
  },
  {
    "reference": "MSQPVDVVILAAGKGTRMKSAHPKVLHRLAGQPLLGHVLAAARGVQARTCVVVTGHQAEAVEAALPALAHGLSVQAVRQSPQLGTGHAVQQALPCLADDGLTLVLSGDVPLTRPETLQALLAAQAAGGPQALALLTVDLPDPHGYGRVLRDAAGAVCGIVEEKDASVAQRQITEVYSGIMAAPTARLRAWLARLDNRNAQGEYYLTDVVRHAVADGTPVVAHRITDPIEVAGVNSPAQLAELERAHQRRVAQTLLAHGVRLADPARLDVRGELVCGQDVEIDVNCVFEGRVVLGDGVRIGAHCVIADAVIEAGAVVHPFTHIDGAGQPVVVGAAARVGPFARLRPGTRLGPQVHIGNFVEVKNTTLAAGAKANHLAYLGDATVGERVNYGAGSITANYDGANKHRTVIEADVHVGSNCVLVAPVTLGAGGTIGAGSTVTKDTPPGALTVARAKAVSIPNWQRPVKRRD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc)3. The protein should have a high affinity for UDP-N-acetyl-alpha-D-glucosamine, acetyl-CoA, Mg(2+).4. The presence of MobA-like NTP transferase domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "VIVLAAGEGTRMKSARPKVLHMIGGRSLLGHAIAAAQGLSPTHLAVVVRHERDRVADHVKQVCPQAVVADQDEVKGTGRATECGLEALPADLSGTVLVTYGDVPLLTTTALQELVVAHEQTASAVTVMTARVPDPTGYGRVLRDETGQLRAIVEHKDASSAQAQIREINAGCYVFRAGVLRAAIDGLSTDNDQNEKYLTDVLAILRGRGERVGAFVASDRWEVAGINSRVQLAEAGRLLNERILRDWMLAGVTLLDPASVYFSHDSVIGQDVTIQPHVYFGPGVTIADNVTINAFSHLEGCTVASGASIGPFARLRPEAEIGGNARIGNFVEVKKASIETGAKVNHLAYIGDARVGAGANIGAGTITCNYDGFLKSFTDIGKGAFIGSNTALVAPVKVGDGAIVGAGSTVSHRVSADALAVERSEQRNVAGWVERHRAGTTAAEAASAARDDTGTDS",
    "response#2": "VVILAAGKGTRMYSDLPKVLHLLAGKPMVQHVIDAAQGVGADKVHLVYGHGGERLQSTLQDPSLNWVLQAEQLGTGHAMQQAAPCFADDEDILMLYGDVPLISTETLQRLRQAKPAQGVGLLTVRLDDPHGYGRIVRNCQGESVQIVEHKDANDAERAIKEVNTGILAAPTSHLKRWLAALHNQNAQQEYYLTDIVAQAVADGVPIATAQPAAALEVEGVNNRVQLAALERAFQARQADSLLLAGVTIVDPASTYIEDTVTIGPDTVVEPQVVIEGVTTFGTECVIGSGSHVTASRFGDRVALKPYCIINDAVVEDGATLGPFCHLRPGCHIGAKAKVGNFAELKKSRIGRGSKVPHVSYVGDTTVGEDVNIGAGTVTCNYDGVRKHETKIADGAFVGTNSSLVAPLTIGEGAYIGAGSTITQDVPPGALAVARGQRXATIASWKRPGASLIAPETVWFSYDTQLGRDVLIEPNVFFGPGVRVADNVKIRANSHIEGAVIGEGCEVGPFARLRPGTVLEEKAKIGNFVETKKAHLGKGAKANHLTYLGDAEIGAKANIGAGTITCNYDGYFKYKTEIGEGAFIGSNSALIAPVKIGRDAIVAAGSAVSRDVADGDLRMVRAEKDK",
    "response#3": "VVILAAGKGTRMYSDLPKVLHLLAGKPMVQHVIDAAQGVGADKVHLVYGHGGERLQSTLQDPSLNWVLQAEQLGTGHAMQQAAPCFADDEDILMLYGDVPLISTETLQRLRQAKPQAGIGLLTVNLENPTGYGRILRAGNNVLGIVEHKDANESQRTINEVNTGILVANGADLKRWLSALNNNNAQGEYYLTDIIAMASRDGERIQTVQPERLSEVEGVNNRLQLSRLERIYQAEQAEKLLLAGVTIVDPASTYIEDTVTIGPDTVVEPQVVIEGVTTFGTECVIGSGSHVTASRFGDRVALKPYCIINDAVVEDGATLGPFCHLRPGCHIGAKAKVGNFAELKKSRIGRGSKVPHVSYVGDTTVGEDVNIGAGTVTCNYDGVRKHETKIADGAFVGTNSSLVAPLTIGEGAYIGAGSTITQDVPPGALAVARGQKTKISGWKRPVKKK"
  },
  {
    "reference": "MQTMGGEHLLLSQLKGSFFLLLLAYFFRGRSPYYARCYRRLAVTPGAITIAIAIATDSIPALAKSKVLVSVCSHTDPCTASCNLIPFPRPFSNSLTRFLFCLGSARFCISFPCFGLSI",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should contain a Helical membrane-spanning region.2. The protein should have a specific function in macroautophagy, autophagy of mitochondrion and should be localized to mitochondrion, mitochondrial membrane in order to carry out this function efficiently.3. The protein must exhibit the following characteristics: Involved in selective mitochondria autophagy (mitophagy).. The designed protein sequence is ",
    "response#1": "RKAALLSRFSDAYSQARLDAQCLLRRCIDKAETVQRIIYIATVEAFHVAKMAFRHFKIRVRKSLTPSYAGSNDFEDAVLDYIICHLDLYDSQSSVNDVIRAMNVNPKISFPPEVDFCLLSNFIQEICCIAFAMQTLDPPLDIAFGADGEIFNDCKYRRSYDSDFTAPLVFYHVWPALMENDCVIMKGEAVR",
    "response#2": "KTVQVIEECATRSDVVYGFAEVKYKQLIPLAPSIQTDPSAKSNMSGVRDTAGSTDGDLTVDATVTLSEEALVLYVKALSLLAKSMDIAGVWWTRKNRGETFGDPVMSRADTTGALVGTRINNVVQWVRNC",
    "response#3": "KTVQVIEECATRSDVVYGFAEVKYKQLIPLAPSIQTDPSAKSNMSGVRDTAGSTDGDLTVDATVTLSEEALVLYVKALSLLAKSMDIAGVWWTRKNRGETFGDPVMSRADTTGALVGTRINNVVQWVRNC"
  },
  {
    "reference": "MVEKRVQPLARDAMAYVLAGGRGSRLKELTDRRAKPAVYFGGKARIIDFALSNALNSGIRRIGVATQYKAHSLIRHMQRGWNFFRPERNESFDILPASQRVSETQWYEGTADAVYQNIDIIQDYGVEYMVILAGDHVYKMDYEWMLQQHVDSGADVTIGCLEVPRMEAVGFGVMHVNDKDEIIAFVEKPADPPPIPDKPDFALASMGIYVFHTKFLLDALRRDAADPNSSRDFGKDIIPYIVKNGKAVAHRFAKSCVRSDFEHEPYWRDVGTIDAYWQANIDLTAIVPELDIYDKSWPIWTYAEITPPAKFVHDDEDRRGSATSSVVSGDCIISGAMLNNSLLFTGVRANSFSKMEGAVILPNVKIGRRAQLKNVVIDHGVVIPEGLVVGEDAELDAKRFRRTESGICLITQPMIDKLDI",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that is capable of catalyzing reactions within the glycogen biosynthesis, Glycan biosynthesis pathway with high efficiency and specificity.2. The protein should be designed to catalyze the following reaction alpha-D-glucose 1-phosphate + ATP + H(+) = ADP-alpha-D-glucose   + diphosphate.3. The protein must exhibit the following characteristics: Involved in the biosynthesis of ADP-glucose, a building block required for the elongation reactions to produce glycogen.4. The protein design should be able to enhance the efficiency of glycogen biosynthetic process. The designed protein sequence is ",
    "response#1": "AMLLAGGKGSRLSGLTKNMAKPAVSFGGKYRIIDFTLSNCSNSGIDTVGILTQYQPLELNSYIGIGSAWDLDRYNGGVTVLPPYAESSEVKWYKGTASSIYENLNYLNQYDPEYVLILSGDHIYKMDYGKMLDYHIEKKADVTISVIEVGWEEASRFGIMKANPDGTITHFDEKPKFPKSNLASMGIYIFNWPLLKQYLEMDDQNPYSSHDFGKDIIPLLLEEKKKLSAYPFKGYWKDVGTVQSLWEANMDLLEVHPTSWMPAKCGDWNTLADRLIPYVQSLGFTHVEFLPVMEHPFGGSWGYQPLGQFAPMARLGTPESFASLVNRLHEVGIGVILDWVPAHFPTDAHGLARFDGTALYEHEDPREGYHYDWNTYIYNLGRNEVRAFLISSAVHWLTYFHADALRVDAVASMLYRDYSRKHGEWIPNMYGGRENLESVSFLQHLSTVVKSRAPGAVLIAEESTAWPGVTRPAHEGGLGFDYKWNMGWMHDTLHYMQEDPVNRSWHHDQMTFGLVYAFSEKFVLPLSHDEVVHGKRSLLGRMPGDEWQRFANLRAYYGFMWTHPGKKLLFMGGEIAQEQEWSEGHGTDWYLLDRPNHQGVQRLVADLNKLYREVPALHKGDWQADGFDWNKADDASNNILVFTRHGDDGSQALCVFNLSGTSQPEYQIGVSGGGSWRLVLNTDDEQYHGANNPLPETIEAEKIDRDGFPYTTTLHSPAMSAQFYVVNK",
    "response#2": "ECHVGSWARRDDGWFLNYRDLAHRLGEYVTYMGYTHVELLGVMEHPFDGSWGYQVTGYYAPTSRLGSPEDFKYLVNHLHSLGIGVLLDWVPGHFPTDEFALAHFDGSPLYEYADPRKGYHYDWNTYIFDYGRNEVVMFLIGSALKWVQDYHVDGLRVDAVASMLYLDFSRTEWVPNIYGGRENLEAIAFLKRLNEVAHHMAPGCLMIAEESTSFPGVTTPTPEGLGFDYKWAMGWMNDSLAYFEQDPIYRKYDHHKLTFFNVYRTSENYVLAISHDEVVHLKKPMVLKHPGDWYAQRADYRAFLAMMWTTPGKKLLFMGQDFAQGSEWNHDAPLPWFHADQPDHRGVMNLVKGWSAVNQFYSEEDLNRLRHCHHHNPHGFYGWHATDDGSVIRTRQIGAEKVELVLGDTQIVMNPIGDDIFAIKLGNREAFDYRLRVTWPGQDPVVTADPR",
    "response#3": "ECHVGSWARRDDGWFLNYRDLAHRLGEYVTYMGYTHVELLGVMEHPFDGSWGYQVTGYYAPTSRLGSPEDFKYLVNHLHSLGIGVLLDWVPGHFPTDEFALAHFDGSPLYEYADPRKGYHYDWNTYIFDYGRNEVVMFLIGSALKWVQDYHVDGLRVDAVASMLYLDFSRTEWVPNIYGGRENLEAIAFLKRLNEVAHHMAPGCLMIAEESTSFPGVTTPTPEGLGFDYKWAMGWMNDSLAYFEQDPIYRKYDHHKLTFFNVYRTSENYVLAISHDEVVHLKKPMVLKHPGDWYAQRADYRAFLAMMWTTPGKKLLFMGQDFAQGSEWNHDAPLPWFHADQPDHRGVMNLVHSFTEDHERLWDVLGANVKTYETTLGQVRGTAFAVWAPNAIGVAVIGGFNGWNASQHAMRSLGGSGIWELFIPNIGPGEVYKFAIQTREGHRRDKADPMARLAELPPATGSIVVWLDGSDAAGNVLAFLRKDGRGEPIAVILNFSGAPHTEYRVGLPFAGGWEELLNTDADVFGGSGVGNFGGVTAVEEPWMGSPASAVLTLPPLGALFLR"
  },
  {
    "reference": "MFLEINFYSTFFQRRPSLWKERCKFEEVDAQGNSISYSHHPPDLTGQRSPLTPTTMRIVDRIPTVVGFRVIPTTVLVLLTYLTIFTLVIVTDWLPEPPKNQNGLDLKQAYTDLRHITAHPHPYNSHYNDAVHDYILSRVRPVAASTSFVHISDDQTSNGSWASPGYGVYFEGTNILVKIDGKSSNGNDGVLFSAHYDSVSTAPGATDDGMGVVTLLQLIDYFAKHRPDRTAIFNINNGEEDWLNGAHAFLQHTWSNLTDTFLNLEGAAAGGRPILFRATSTSPVRAFRSDYVPHPHANVISSDAFARGVIRSGTDYEVYTGAGAEMEGLDVAFYKGRSRYHTKYDAVPYTNGGERSLWAMMETAQGAGNALLNAKRHKQDQGSGGTPVYFDLVKAELVIFYLNDLLIYNVVSLVVGPISLIFFVVCEYVLRNERARQPNGHPVSRPSVLEWLKQRSWLRALWRRSKFWIALVITIALQALLVWGYLAFNSFTVYSSPYLVLISFFSLAYLSLVIPLTFTFNQTQSPTAKYIAPEREKHTLLIQVYIFTWILLLFSTIAVARAQVGGLYFVTAWNTGVWIACLLAAVEGMMLPVPQGGPRVRFHSAHHHHHHEHEEDQDADDDDREQRQPPTEATPLIGYSRASLRKPQEGGVVGWWIVHLLLTIPAPVLLIAQMGSLLLDSLPQTLADGSPAYVVYAAASLTAVLLAVPLTPFSGKLHRGLFFLFFLSFLIVTAYLWLAFPFSSADPLKVFFQQKVTLSNVVSERHSS",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.2. The designed protein should have at least Helical; Name=2, Helical; Name=7, Helical; Name=1, Helical; Name=3, Helical; Name=9 transmembrane domains to ensure proper localization.3. The protein can be targeted to vacuolar membrane and perform metalloexopeptidase activity.4. The Vacuolar topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.5. The protein must exhibit the following characteristics: May be involved in vacuolar sorting and osmoregulation..6. The protein requires Zn(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "KEQELLHQAWVDLQHIAKYEHAYGSSGNDYVHDYLESRIVSAVAHKSYVEYDNDLNYTNNIMFGSRSELSGNSFNSVSYYESNNLVVRINGTDETLPALLLSAHFDSVPSSFGVTDDGMGIASLLGVLYYYTGKSTARPRRTIVLNFNNDEEFGLYGATSFLSHPWATGVHYFLNLEGTGAGGKAILFRGTDYGITKYFKGVRYPYGTSIFQQGFNNHLIHSETDYKIYKEKGGLRGLDVAFYKPRDLYHTAGDNIKNIDIKSLWHMLSNALDFTAIVTKGKIDLDADSLDSESSKSNTDTAVYTSFLNFFFAFT",
    "response#2": "KEQELLHQAWVDLQHIAKYEHAYGSSGNDYVHDYLESRIVSAVAHKSYVEYDNDLNYTNNIMFGSRSELSGNSFNSVSYYESNNLVVRINGTDETLPALLLSAHFDSVPSSFGVTDDGMGIASLLGVLYYYTGKSTARPRRTIVLNFNNDEEFGLYGATSFLSHPWATGVHYFLNLEGTGAGGKAILFRGTDYGITKYFKGVRYPYGTSIFQQGFNNHLIHSETDYKIYKEKGGLRGLDVAFYKPRDLYHTAGDNIKNIDIKSLWHMLSNALDFTAIVTKGKIDLDADSLDSESSKSNTDTAVYTSFLNFFFAFL",
    "response#3": "HEQAVLSRSWESLQKIARTKHTYTSEGNDEVHAYLEAHIASLVAKKPYMELDTDKNGTRRVMFDVKYLSYDSVSYYESNNLVVRVNGSDSSLPALLVSAHYDSVPTSYGVTDDGMGVASMLGLLEHYSSVAQPKRTIIFNFNNNEEFGLYGAQAFLAHPWFSQIAYFLNLEGTGAGGKAILFRGTDYGIVRHFSSVRFPFASSLFQQGFNNRLIHSETDYSVYIKAGLRGLDLAFYKPRDIYHTTRDSIQNTNIKSLWHMLSSSLDFVEHVSSQTIDLDEEVHAEAGKRDLALYTSFWR"
  },
  {
    "reference": "MIQRESRLVVADNSGAKEVLVINILGGSTGFYANIGDIVIVTVKKAISSGAIKAGDVLKAVIVRTKYGLRRKDGSYIKFDENAVVIIKDDLTPRGTRIFGSIARELREKKFTKIISLAQEVL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have structural constituent of ribosome to facilitate translation. The designed protein sequence is ",
    "response#1": "METR",
    "response#2": "EIDVHVPIHLVGEAAGVKDGGVLQQAMHQLSIRVLPSNIPPSIDVDITNLQVGDVITVSHIQTNGAYEINHDPTEVIASILPPS",
    "response#3": "EIDVHVPIHLVGEAAGVKDGGVLQQAMHQLSIRVLPSNIPPSIDVDITNLQVGDVITVSHIQTNGAYEINHDPTEVIASILPPVS"
  },
  {
    "reference": "MSKTYTVAVVGATGAVGETMLKVLAERQFPIGKLHVLASERSAGSKVDFGARQLVVEDLATFDPTGVDIALFSAGGSVSKEYAPKFAAAGAVVIDNSSTFRGDADVPLVVSEVNPEAAKNRPRGIIANPNCSTMQLMVALAPIHRAVGIERINIATYQSVSGTGAKAMAELGKQTADLLNFREIEPKVYPVQIAFNVIPHGGDFLDNGYTTEEWKLLTETRKILGDDNIQVNATVVRVPVFYGHSEAVHIETREKISVEKARELLAAQPGLVLVDERKAGGYPTPVTHASGNDPVFVGRLREDVSHPRGLDLWVVSDNIRKGAATNAVQLAELVIAEG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for NADP(+) binding.2. The protein should be able to modulate lysine biosynthetic process via diaminopimelate in a way that leads to a desirable outcome.3. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.. The designed protein sequence is ",
    "response#1": "LKPIHEAYGLDRVIVSTYQAVSGAGVEAIEELDQQNASLLKGEKPQAAILPSSGDKNHYQIAYNVIPQIDLFAEEGYTFEEWKMINETKKIMGLPDLKVSATCVRVPVKYGHSESVYIEIDRDDVTVTDLQALLAQADGVIVQDDPANQLYPLATESEDKKETFVGRIRKDPDVANGFHLWIVSDNLIG",
    "response#2": "LKPIHEAYGLDRVIVSTYQAVSGAGVEAIEELDQQNASLLKGEKPQAAILPSSGDKNHYQIAYNVIPQIDLFAEEGYTFEEWKMINETKKIMGLPDLKVSATCVRVPVKYGHSESVYIEIDRDDVTVTDLQALLAQADGVIVQDDPANQLYPLATESEDKKETFVGRIRKDPDVANGFHLWIVSDNLIE",
    "response#3": "LKPIHEAYGLDRVIVSTYQAVSGAGVEAIEELDQQNASLLKGEKPQAAILPSSGDKNHYQIAYNVIPQIDLFAEEGYTFEEWKMINETKKIMGLPDLKVSATCVRVPVKYGHSESVYIEIDRDDVTVTDLQALLAQADGVIVQDDPANQLYPLATESEDKKETFVGRIRKDPDVANGFHLWIVSDNLIE"
  },
  {
    "reference": "VSCVLDVPFIFLTSFPPPHIFQVVHILGDQFPYKVVSKKIDLPEYQGEPDEVCIQKCKEAARQIDGPVIVEDTCLCFRALGGLPGPYIKWFLNQLKPTGLHTLLAGFEDKSAWALCTFAFSAGRGEPVQLFRGKVEGMIVEPRGPPDFGWDPCFEPDGYDKTYAELPKEVKNSISHRYRALAAMSEHFAKINRTSQESKKNKQNE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions..2. A protein with metal ion binding, ITP diphosphatase activity is needed to enhance nucleotide metabolic process in a specific context. The designed protein sequence is ",
    "response#1": "MPKIQLGN",
    "response#2": "MENK",
    "response#3": "MMKIFHA"
  },
  {
    "reference": "MMELSGKKIVLGLSGGVACYKAAELCRALTKEGASVQVVMTDAATHFITPVTMQALSGKPVFSSQWDPRVNNNMAHIDVTRDADAILIAPCSADFIVKLAHGVCDDLLSTMCLARPRTLPLLIAPAMNVEMWSNPATQRNVDQVRADGITVFGPAAGDQACGETGLGRMLEPEQLLAEMIAAFQPKVLAGKRILVTAGPTFEAIDPVRGITNLSSGKMGYAVARAAREAGAEVTLVSGPTALATPFGVDRHDVKSAQQMFDAVMDHIGGQHVFISVAAVADWKVVNPSAQKVKKTGDGSVPELKFEQNPDILASVATRTSLAGWPYCVGFAAESENLIEYGASKRERKGIPLLVGNIGHNTFGQDDNTIILFDEHGHTILPRADKLTLARQLISEIAKRIDKNSLFQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein with metal ion binding, phosphopantothenate--cysteine ligase activity, FMN binding is needed to enhance pantothenate catabolic process in a specific context.2. The CTP binding site should be located in a region of the protein that is accessible to the ligand.3. The protein's Proton donor must be carefully engineered to ensure that the amino acids involved in H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2 are properly positioned for optimal catalysis.4. The designed protein have DNA/pantothenate metabolism flavoprotein C-terminal domains with specific sequence motifs that are crucial for its activity.5. We require a protein that can specifically bind to the metabolites produced in the Cofactor biosynthesis pathway.6. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.. The designed protein sequence is ",
    "response#1": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALRVLGAPAGPAGDGLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHVLGADGSLAGRHVVVSAGGTREPLDPVRFLGNRSSGRQGYALARTAVARGARVTLVAANTALPDPAGADVVRVGTAVQLREAVLKAAADADAVVMAAAVADFRPARYAQGKIKKRDGQEPEPVELVRNPDVLAELSSTRPRPGQVVVGFAAETDDVLVNGRAKLARKGCDLLVVNEVGEEKTFGSTENEAVVLGADGSAVEVPHGPKEVLADQVWDAVVRLLE",
    "response#2": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALALAAAVLGGGRLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHMLFADGSPQVLVMVSGSVAAIKAPATLRRLREQGVQTGVIATQAALHFVTELSLSTAGNCRVATDQTWFAPQPQAQHLTLARCDAAVIVGAGADALARAAQGRAEDLLSATLLSVRAPVLWVPAMNEAMWQHPAVQRNVAQLRADGHHFLGPVSGAFGTAGEGAGMGRMAEPEDIAAQVVEAVLARLRRAADTSG",
    "response#3": "MRIVLGVGGGIAAYKAALLLRLFTEDGHEVTVVPTDAALRFVGAPTWEALSGRPVQTDVWSNITDVPHVRIGQEADLVVVAPTTADLLAKAAHGLAGDLLTNVLLTARCPVVLAPAMHTEMWEHAATRANVAILRERGVTVVEPASGRLTGSDTGPGRLPEPEAIHDAALRTLALPPPPDTLAGRHVVVSAGGTREPLDPVRFLGNRSSGRQGYALARTAVARGARVTLVAANTALPDPAGADVVRVGTAVQLREAVLKAAADADAVVMAAAVADFRPARYAQGKIKKRDGQEPEPVELVRNPDVLAELSSTRPRPGQVVVGFAAETDDVLVNGRAKLARKGCDLLVVNEVGEEKTFGSTENEAVVLGADGSERLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHLLGADGSETLAGRRVLISTGGTREPLDPVRYLGNRSSGKQGLALAQAAADRGAHVTLVAANLVLPVPAGIEVVPVETALHLQGEMTRRAPDHDVVVMVAAVADFRPAEYAASKIKKTHDPADPDAAPTIQLVRNPDVLAGLVEARGCATSPVIVGFAAETGDATGSVLELGRAKLARKGCDLLVVNEVGVDKTFGADDTTVHLLGADGSEV"
  },
  {
    "reference": "MLKGKKILLGVTGGIAAYKSPGICSLLRKLGAEVKVVMTKNATEFVTPLTFQTMSNNVVHGEMFNQLFNMDVEHISLAKWADIIVIAPATANIIGKFANGIADDMLSTVLMASRSKVMLAPGMNTVMLNSEANQKNMQTLRDRGVIILDTKEDLLACNDYGSGKMLEPSEIVDEIDRALTEKDLEGKHVVITAGPTREALDPVRFISNYSSGKMGYALADNAKKRGAKVTLISGPTKIEVPKVDNFVRVDSTRQMFDAVGKYFDDCDILIKAAAPSDYRPKTYSEEKIKKGSDSQMNLIEMEKNPDIAAHYGSLKKSQVIVGFAAESTNIYEYAKEKLIKKNFDIIVVNNIKKEGAGFGSDTNIVSIISQDKTDDLEIMSKTELAREILDRAKKLMS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be able to modulate pantothenate catabolic process, coenzyme A biosynthetic process in a way that leads to a desirable outcome.2. The presence of Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.3. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.4. The protein design should consider the endogenous proteins and enzymes present in the Cofactor biosynthesis, coenzyme A biosynthesis pathway to avoid interference with native metabolic processes.5. The designed protein should be able to compete with endogenous proteins or inhibitors for CTP binding. The designed protein sequence is ",
    "response#1": "LRDAGSDVRVVMTRGARAFVTPLTFQAVSGNPVRTELLDPDAEAGMGHIELARWADRILVAPATANFMARFAQGLADDLLSTLCLATTAPVALAPAMNRQMWAHPATQANCAVLEERGVRLIGPESGSQACGETGPGRMTEPAELVAAVLGVAGGIAAYKAAELLRRLTEAGHRVRVVPTRSALAFVGAATWAGLSGEPVAVDVWSDVHEVPHVRLGREADLVVVAPATADLLSRAAAGRVDDLLTATLLTARCPILYAPAMHTEMWLHPATQANVATLRERGAVVLEPAEGRLTGFDSGPGRLPEPAEIAAVVLRTLAVAG",
    "response#2": "LRDAGSDVRVVMTRGARAFVTPLTFQAVSGNPVRTELLDPDAEAGMGHIELARWADRILVAPATANFMARFAQGLADDLLSTLCLATTAPVALAPAMNRQMWAHPATQANCAVLEERGVRLIGPESGSQACGETGPGRMTEPAELVAAIEGALLGPRDLRGERVLITAGPTREAIDPVRFIGNRSSGRMGFAVAAAAARAGAKVVLVAGPCSLATPGGVERVDVESAREMHEAVMQRAGDCDIFIATAAVADYRVDRVPEQKIKKSDQPVALTLVPNPDILQAVAGLPRAPFTVGFAAETERLAEYAEAKRARKGLDMIAANLVGGGRDTGFDSADNALEVFWPSGKHSLERTSKARIATRLADLVVERL",
    "response#3": "KRILLGITGGIAAYKCPDLVRHLKKAGAQVHVVLTDSASHFVTKMTLQAVSGNPVSSDLFDPSAELSMSHIELAKWADFVLIAPATANIIAKMANGIADDLLSTICLASSAPIAIAPAMNQQMYKATATQHNLSVLASRNTLILGPDNGYQACGDIGPGRMLEPHTIVEEIAEALITSDYFAGKKILITGGSTREYIDPVRFITNNSSGKMGISIARVAKAMGAYTNLISGEILTNLPILDKYQKVETTEEMLKATKEAFKASDILIMAAAPVDFKPETISTTKIPKKKEITLKLLETPDILKEISKEKGNKIIVGFALQTENLENKALKKMKEKNMDIVVANRKEPMVKVLEGSATGAMIRDAILNQD"
  },
  {
    "reference": "MAKSNKSDGAQKQQTLASSFELEGKGLHTGLSIKVKFSPASEGHGVKICRVDLPNRPTIPALAEFVTKTQRGTVLSNKDLQVSTVEHALSALYAMGVDNCLIEVNAPELPIFDGSASIYVEHIRRVGIKEQNAVREVYVVKSKIEVKDEQGVSQITILPDSSFGVHVLISFNSKVLSNQYATLQDVADFADEIAPARTFVFVREIESLLDNDLIKGGDLDNAIVIYDQEFSQDKMDKLAANMGTVSKPANELGYINNKPLKYNNEPARHKLLDLIGDLALIGRQLQGRVIATCPGHSINTKMAKLIRKDIRLNESQSPVYNPNEEPVLRIQDLKSLLPHRYPMLLVDKIIEIGPDHIVGVKNVTHNEPFFIGHFPDEPVMPGVLQVECMAQIGGVLILNQLENPSEYSTYFLTIDNVKFRYKVVPGDTLVAKVSLLGPVRRGIANMRGLAFVGEKLVCEAEFMAQIVRNAQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Involved in unsaturated fatty acids biosynthesis; Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs..2. The protein design must be able to perform 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity in order to contribute to lipid A biosynthetic process.3. The protein requires Zn(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "MATLSKERIKHVARLARLELNDEDIPALTGQLNEIIKMVEQLNELDTEGVEPTSHVLPLENVTRPDVVTEGASRDELLAGAPAAREGRFAVPKVIE",
    "response#2": "MERKEFDLR",
    "response#3": "MMKRIYLATRNQGKYREFADVFGNEWDLIGLNDFPELPKVVEDGNTFAENAAKKARATCELTGLLTLADDSGLEVEALGGAPGVRSARYAGGAGDDEANNAKLLEALEDVPSGQRTARFRCVIAFARPDGRVELVEGVCEGRIGLEPRGENGFGYDPIFFVPELGRTMAELTLEEKNRISHRKKALERVREFLKERRE"
  },
  {
    "reference": "MKVKGTRKNYRHLWIWGTWLLGMLMICSAQDNLWVTVYYGVPVWKEATTTLFCASDAKGYNTEAHNVWATHACVPTDPSPQEVRLINVTEDFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVSLKCTNLTLRNDTNTSSSGRVEXEREEIKNCSFNITTSIRNKMQKEYALFYKLDLVPIDNATSYTLISCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNDKTFNGSGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSENFTNNAKTIIVQLNSSVEINCTRPNNNTRKSITIGPGRAFYTTGDIVGDIRQAHCNLSRTNWTNTLQQIVXKLQEQFGNKTIVFNQTSGGDPEIVMHTFNCGGEFFYCDTAQLFNSTWLGNETEDTNTTNDNITLPCRIKQIINRWQEVGKAXYAPPIEGQXRCSSNITGLLLTRDGGSNNTESEIFRPGGGDMRDNWRSELYKYKVVRIEPLGVAPTKAKRRVVQREKRAVTFGAMFLGFLGAAGSTMGAASLTLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTAVPWNASWSNGRNLSEIWDNMTWMEWEREIENYTGLIYNLLEQSQNQQEKNEQELLELDKWASLWNWFDISHWLWYIRLFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTRPPAPRGPDRPEGTEEEGGERSRATSGHLVTGFLAIIWVDLRSLCLFSYHRLRDL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; Transmembrane protein gp41: Acts as a class I viral fusion protein2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. Incorporate a signal peptide in the protein design.4. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells.5. The protein design should be able to enhance the efficiency of viral protein processing, virion attachment to host cell. The designed protein sequence is ",
    "response#1": "MRVMGIRRNCQRWWIWGSLGFWMLMICNGVGNLWVTVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQEIVLGNVTENFNMWKNDMVDQMHEDIISIWDQSLKPCVKLTPLCVTLNCTDAKVNSTTINNTSSNVSNSNELLKCSFNMTTVIRDRRQKMDALFYRLDVVPITDGQNSTQNSSYRLINCNTSAITQACPKVTFEPIPIHYCAPAGFAILKCNDKEFNGTGPCKNVSTVQCTHGIRPVVSTQLLPEMAVLAEGEVMIRSENLTNNAKVIIVQLNESVAINCTRPNNNTRKGIHIGPGQAFYATDNIIGNIRQAYCNVSRTGWNKTLQNVVTQLQKHFKNATIVFDKHSGGDLEITSHTFNCRGEFFYCNTSKLFNSSWNGTEDPIILPCEIKQIINMWQRVGQAIYAPPIPGVIRCNSNITGILLTRDGGTNSTNETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTEAKRRVVERGKRAVGIGAVFLGFLGAAGSTMGAAAVTLTVQARQLLSGIVQQQNNLLRAIEAQQHMLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNKSQTAIWENMTWLQWDREISNYTNIIYELLEESQNQQEKNEQELLALDKWANLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSIINRVRKGYSPLSFQTLTPNPRDLDRLGRIEEEGGEQDRDRSIRL",
    "response#2": "MRVKGIRKNYQHLWKWGTMLLGMLMICSAAEKKWVTVYYGVPVWKEATTTLFCASDAKAYDTEXHNVWATHACVPTDPSPQEVFLNVTENFNMWKNNMVEQMHEDIINLWDQSLKPCVKLTPLCVKLNCTNYNNGTNNTNNTSSNVSNSNELLKCSFNMTTVIRDRRQKMDALFYRLDVVPITDGQNSTQNSSYRLINCNTSAITQACPKVTFEPIPIHYCAPAGFAILKCNDKEFNGTGPCKNVSTVQCTHGIRPVVSTQLLPEMAVLAEGEVMIRSENLTNNAKVIIVQLNESVAINCTRPNNNTRKGIHIGPGQAFYATDNIIGNIRQAYCNVSRTGWNKTLQNVVTQLQKHFKNATIVFDKHSGGDLEITSHTFNCRGEFFYCNTSKLFNSSWNGTEDPIILPCEIKQIINMWQRVGQAIYAPPIPGVIRCNSNITGILLTRDGGTNSTNETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTEAKRRVVERGKRAVGGLGAVFLGFLGAAGSTMGAASLTLTVQAKQLLFGIVRQQNNLLRAIEAQQRLLQLTVWGIKQLQARVLAVERYLQDQRLLGIWGCSGKLICTTNVPWNTSWSNKSHDEIWNNMTWMEWDREINNYTQLIYNLLEESQIQQEKNEQELLKLDSWASLWNWLDISNWLWYIKIFIMIVGGLVGLRIILCVLSVVNRVRQGYSPLPFQTWLQAREPEPRRGVEEGGGE",
    "response#3": "MKVKEIRKNYQHLWRWGTMLLGMLMICSAAEKKWVTVYYGVPVWKEATTTLFCASDAKAYDTEXHNVWATHACVPTDPSPQEVFLNVTENFNMWKNNMVEQMHEDIINLWDQSLKPCVKLTPLCVKLNCTNYNNGTNNSTGNVTSDMRNCSFNATTEIRDRTQNVHALFYRLDIVQLDSNNNDTYRLINCNTSAITQACPKVSFDPIPIHYCTPAGYAILKCNDKTFNGTGPCRNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTDNVKTIIVHLNESVEIECTRPGNNTRKSVRIGPGQTFYATGDIIGDIRQAHCNISNKWHDTLQKVGKILRERYSNKTIKFEPSSGGDLEITTHSFNCRGEFFYCNTSKLFNSTYNGTDNGNSSSSNSSSIITIACRLKQIVKLWQEVGRAMYAPPIAGNITCKSNITGILLVRDGGTNGTEETETFRPGGGNMRDNWRSELYKYKVVEIKPLGIAPTTAKRRVVEREKRAVGLGAVFLGFLGAAGSTMGAAAVTLTVQARQLLSGIVQQQNNLLRAIEAQQHMLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNKSQTAIWENMTWLQWDREISNYTNIIYELLEESQNQQEKNEQELLALDKWANLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSIINRVRKGYSPLSFQTLTHHQREPDRPERIEEGGGEQDRDRSVRLVSGFLALAWDDL"
  },
  {
    "reference": "MGGKWSKSSIVGWPAIRERIRRTNPAAERVGARESRTRTEPAAEGVGAASRDLERHGALTSSNTRHTNPDCAWVEAQEEEEEVGFPVRPQVPLRPMTYKGALDLSHFLREKGGLDGLIWSQRRQDILDLWIYNTQGYFPDWQNYTPGPGIRYPLTFGWCFQLVPVEPEEVERVTEGEDNCLLHPMNQHGMDDPEKEVLMWKFDSRLAFEHRARVKHPEFYKDC",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The ideal protein would be one that plays a critical role in suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host autophagy through SH3 domain binding, GTP binding within virion component, host cell plasma membrane, host cell Golgi membrane, extracellular region.2. The protein's functional design necessitates the inclusion of a Diacidic; necessary for CD4 internalization, PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Dileucine internalization motif; necessary for CD4 internalization motif.3. The protein must exhibit the following characteristics: Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike.4. Prioritize Basic and acidic residues, Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "MGNKWSKGWPAVRERIRRTEPAAEGVGAASQDLDKHGALTSSNTAANNADCAWLEAQEEEEEVGFPVRPQVPLRPMTYKGAVDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVDPEKVEEANEGENNSLLHPMCQHGMDDPEKEVLVWKFDSRLAFHHMARELHPEYYKDC",
    "response#2": "MGGKWSKSSVVGWPAIRERIRRTEPAADGVGAASQDLDKYGALTSSNTATNNAACAWVEAQEEEEEVGFPVRPQVPLRPMTYKAAVDLSFFLKEKGGLEGLIYSKKRQDILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVEPEKVEEANEGENNCLLHPMSQHGIEDADRETLRWEFDSRLALRHIARERHPEFYKDC",
    "response#3": "MGNKLSRWPAVRERIRRTEPAAEGVGAASQDLDKYGALTSSNTAGNNADCAWLQAQEEENEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLEGLIYSKKRQEILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCYKLVPVDPREVEEANKGENNSLLHPICQHGMEDEDREVLMWKFDSALARRHIARELRPEFYKDC"
  },
  {
    "reference": "MAFNGTWNVYAQENYENFLRTVGLPEDIIKVAKDVNPVIEIEQNGNEFVVTSKTPKQTHSNSFTVGKESEITSMDGKKIKVTVQLEGGKLICKSDKFSHIQEVNGDEMVEKITIGSSTLTRKSKRV",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must have high specificity towards lipid binding.2. The cholate binding site should be located in a region of the protein that is accessible to the ligand.3. For general function, the protein need meet that Involved in intracellular lipid transport The designed protein sequence is ",
    "response#1": "SESRMLYFWLSEHILNSLASAAFLDERLVMNISGKKLQALFEIEDTEVQQKAVQLIFQGTSYNDSVAKVWSLTHPEISLQPEGTVVKSLVAVEINIFPPGEESLMVLYMEKEITVTIQAAYAEKKLILQPLDSRIEFKVFKWAADPSENDQSIRNFLQKMISVVGIPEVISRIEPALTSLMNSKGLHLFEIRNPEIITRKGYLIVQLM",
    "response#2": "FQDCGKGEVESLEVEGCSGDYCVIHKGKKLDLAISVTSNQDSANLKLDIVADINGVQIEVPGVDHDGCHYVKCPIKKGQHFDVKYTYSIPAILPTTKAKIIAKIIGDKGLGGCIVINP",
    "response#3": "SESRMLYFWLSEHILNSLASAAFLDERLVMNISGKKLQALFEIEDTEVQQKAVQLIFQGTSYNDSVAKVWSLTHPEISLQPEGTVVKSLVAVEINIFPPGEESLMVLYMEKEITVTIQAAYAEKKLILQPLDSRIEFKVFKWAADPSENDQSIRNFLQKMISVVGIPEVISRIEPALTSLMNSKGLHLFEIRNPEIITRKGYLIVQLI"
  },
  {
    "reference": "MGPSNPAMAYFKPSTRDTMDPCSGNAADGSIRVRFRGGIERWKECVNQVPERCDLSGLTTDSTRYQLASTGFGDASAAYQERLMTVPVDVHAALQELCLERRVSVGSVINFSVHQMLKGFGNGTHTITASLHREQNLQNSSPSWVVSPTIVTHENRDGWSVAQAVESIEAGRGSEKESVTAIDSGSSLVKMGLFDLLVSFVDADDARIPCFDFPLAVIVRECDANLSLTLRFSDCLFNEETICNFTDALNILLAEAVIGRVTPVADIELLSAEQKQQLEEWNNTDGEYPSSKRLHHLIEEVVERHEDKIAVVCDERELTYGELNAQGNSLARYLRSIGILPEQLVALFLDKSEKLIVTILGVWKSGAAYVPIDPTYPDERVRFVLDDTKARAIIASNQHVERLQREVIGDRNLCIIRLEPLLASLAQDSSKFPAHNLDDLPLTSQQLAYVTYTSGTTGFPKGIFKQHTNVVNSITDLSARYGVAGQHHEAILLFSACVFEPFVRQTLMALVNGHLLAVINDVEKYDADTLLPFIRRHSITYLNGTASVLQEYDFSDCPSLNRIILVGENLTEARYLALRQRFKNRILNEYGFTESAFVTALKIFDPESTRKDTSLGRPVRNVKCYILNPSLKRVPIGATGELHIGGLGISKGYLNRPELTPHRFIPNPFQTDCEKQLGINSLMYKTGDLARWLPNGEVEYLGRADFQIKLRGIRIEPGEIETMLAMYPRVRTSLVVSKKLRNGPEETTNEHLVGYYVCDSASVSEADL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The presence of Carrier domains is critical for the interaction of the designed protein with its intended targets.2. The protein must exhibit the following characteristics: The tripeptide ACV is then cyclized to form isopenicillin N (IPN) by the isopenicillin N synthase ipnA that forms the beta-lactam nucleus.3. A protein should be able to manipulate the activity of enzymes in the Antibiotic biosynthesis pathway, either by activating or inhibiting their function.4. The protein should be optimized for stability and solubility when expressed in vacuolar membrane where it will be involved in penicillin biosynthetic process. The designed protein sequence is ",
    "response#1": "CKTPNGALQGQNWDFFTATKENLIQLTICQPGLPTIKMITEAGIIGKVGFNSAGVAVNYNALHLHGLRPTGLPSHLALRMALESTSPSEAYEKIVSQGGMAASAFIMVGNAHEAYGLEFSPISLCKQVADTNGRIVHTNHCLLNHGPSAQELNPLPDSWSRHGRMEHLLSGFDGTKEAFAKLWEDEDNYPLSICRAYKEGKSRGSTLFNIVFDHVGRKATVRLGRPNC",
    "response#2": "CKTPNGALQGQNWDFFTATKENLIQLTICQPGLPTIKMITEAGIIGKVGFNSAGVAVNYNALHLHGLRPTGLPSHLALRMALESTSPSEAYEKIVSQGGMAASAFIMVGNAHEAYGLEFSPISLCKQVADTNGRIVHTNHCLLNHGPSAQELNPLPDSWSRHGRMEHLLSGFDGTKEAFAKLWEDEDNYPLSICRAYKEGKSRGSTLFNIVFDHVGRKATVRLGRPNC",
    "response#3": "CKTPNGALQGQNWDFFTATKENLIQLTICQPGLPTIKMITEAGIIGKVGFNSAGVAVNYNALHLHGLRPTGLPSHLALRMALESTSPSEAYEKIVSQGGMAASAFIMVGNAHEAYGLEFSPISLCKQVADTNGRIVHTNHCLLNHGPSAQELNPLPDSWSRHGRMEHLLSGFDGTKEAFAKLWEDEDNYPLSICRAYKEGKSRGSTLFNIVFDHVGRKATVRLGRPNC"
  },
  {
    "reference": "MQDLLLAKVESAMQASQVSALAGQTATVTKVSAATNLATITPTAAGQAPIIVKLDATRQVVELQALVGKTVMVGKTPAAIGGIGNWIALTPVTGAKAAAAATGAGQLVMMKVEGTAAAVNLPALAGKSFTIAQPPVAAGTKAAGMLYLNPVGGGDLIAINVQNAATQTGGLVGKTFVVAPSPVIGGTTGKFLVLKPLTAGAGKAVGGGAIAAKFIPAAVTGTGGAAAVGAGSASSLLTAGAGTVTPITAAGTGSAMLSAKGLGLGLGLGLGAWGPFLLGAAGLAGAAALYVWARRRHGTPDLSDDALLAAAGEE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: Increased protein levels allow crystal growth in the minor axis but not increased numbers of crystals.; May help control oxido-reduction reactions surrounding the crystal lattice of the forming magnetite mineral (Probable); Helps regulate magnetite crystal morphology, probably in a single growth axis; Expression in a deletion mutant restores growth of spherical (wild-type) magnetite crystals.2. The protein can be expressed in magnetosome membrane and perform metal ion binding without triggering undesirable cellular responses.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "IYLLMTVGALMAALSISIQPLLLDKIFGIAFEKEGAVNADIQVVAEIVSIVCVGWFGLLSDRIGRVRIIALGFLIAVVGAAVSLLSLQVGLAFGAAGLVLFYLTRVLLTVGADTVQLQLSTLVGDVSSRANRPRLMGNLVFMMVFGGTMLAAIVMQMADYPGGVFLIMCLPLLAGIAGFQLTRRNLRDVAPPQPASEDDEHPLRQVWTVITSDPRMQLAFAAAFYTRADVIILSLFFSLWCISVSDLVGVTRTFATAHAAVMIGLLGLAVLAAVPLWRSFIERHSRISAIGASLSLAALGYIWLGMFANPFNWLVALPLLMVGIGHAGCFVTLQVLTVDASPKPILGAMVGAGYLVGGLGTVMLVQSGGYYFDALGPRAPFILMGTGKMLVTLYAAWLLANGE",
    "response#2": "IYLLMTVGALMAALSISIQPLLLDKIFGIAFEKEGAVNADIQVVAEIVSIVCVGWFGLLSDRIGRVRIIALGFLIAVVGAAVSLLSLQVGLAFGAAGLVLFYLTRVLLTVGADTVQLQLSTLVGDVSSRANRPRLMGNLVFMMVFGGTMLAAIVMQMADYPGGVFLIMCLPLLAGIAGFQLTRRNLRDVAPPQPASEDDEHPLRQVWTVITSDPRMQLAFAAAFYTRADVIILSLFFSLWCISVSDLVGVTRTFATAHAAVMIGLLGLAVLAAVPLWRSFIERHSRISAIGASLSLAALGYIWLGMFANPFNWLVALPLLMVGIGHAGCFVTLQVLTVDASPKPILGAMVGAGYLVGGLGTVMLVQSGGYYFDALGPRAPFILMGTGKMLVTLYAAWLLANGTR",
    "response#3": "GDWAFTFLIISLAMRPVQEITGIKTLAKYRRMIGLFAFFYAVMHVLAYVALEWALNLGDMMGDIYKRPFILLGLVAFALLIPLAFTSANSQIKRIGGKRWKKLHSATYVINAP"
  },
  {
    "reference": "MSRSDTARPFVKWAGGKRALAPTLFAHMPQTFGSYFEPFVGGGALFWHLCACTRVRLHDIYLSDINWPLLCAYAAVRDRVEELIVRVGQHIACHTPTYYRLARRKFAVCEHPLEVAALFLYLNRSCYNGLYRVNKAGQFNVPLGRAAPASPFLNTTAPTPRSTQPAAQVGHLAIRIDEENLRSCARALANTTLNCQHFSCIQPARGDFVYLDPPYLGTFSAYDKTGFDRAAHESLAAFCMHLDARGVLFMLSNSDCPEVRAWYRPFRVQQLNAPRCIARSAHARGKRCEVLITNYPCADTATP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. We need a protein that can perform a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an   N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-   homocysteine with high efficiency.2. The protein must exhibit nucleic acid binding.3. For general function, the protein need meet that An alpha subtype methylase that recognizes the double- stranded sequence 5'-GATC-3' and methylates A-2 on both strands (Probable); Directly involved in methyl-directed DNA mismatch repair, DNA replication, and gene expression . The designed protein sequence is ",
    "response#1": "TVEGKWTKATLSIDELQLIKRVIAHPDVRKFKDIAKVDVGRYCDGANNYFLVDNETVKLYKLERFAHPMFGRSQHCPGIIYDEKQHIENQEKGLPTNFLYIDEEFEYLSKSVKNYIKLGEVEEYHKRYKCRIRKPWFKVPSVYSTEIGMLKRCHDAPRLIHNRVRAYTTDTAYRVSSTVTSTENLVCSFLNPITVITAELEGLFYGGGVLELVPSEIEKLYILIVEGLEHNVEELNLLIKDGQIERVIRQQGSLILGTLGFTQEENEKLVEIGRSLEIEGYVD",
    "response#2": "TVEGKWTKATLSIDELQLIKRVIAHPDVRKFKDIAKVDVGRYCDGANNYFLVDNETVKLYKLERFAHPMFGRSQHCPGIIYDEKQHIENQEKGLPTNFLYIDEEFEYLSKSVKNYIKLGEVEEYHKRYKCRIRKPWFKVPSVYSTEIGMLKRCHDAPRLIHNRVRAYTTDTAYRVSSTVTSTENLVCSFLNPITVITAELEGLFYGGGVLELVPSEIEKLYILIVEGLEHNVEELNLLIKDGQIERVIRQQGSLILGTLGFTQEENEKLVEIGRSLEIEGYVDC",
    "response#3": "TVEGKWTKATLSIDELQLIKRVIAHPDVRKFKDIAKVDVGRYCDGANNYFLVDNETVKLYKLERFAHPMFGRSQHCPGIIYDEKQHIENQEKGLPTNFLYIDEEFEYLSKSVKNYIKLGEVEEYHKRYKCRIRKPWFKVPSVYSTEIGMLKRCHDAPRLIHNRVRAYTTDTAYRVSSTVTSTENLVCSFLNPITVITAELEGLFYGGGVLELVPSEIEKLYILIVEGLEHNVEELNLLIKDGQIERVIRQQGSLILGTLGFTQEENEKLVEIGRSLEIEGYVSPR"
  },
  {
    "reference": "MKNGIDFVETEAYARIYNFILMVDDSIKNSEQRQSKHHMDVLADITRIVSETEKDPSPQRYANMAAKTVFKKIYDTYDDEYLRNSFGNQIRLDYGTGHELNFLCYLYAQYCRGSIGIDCVFTILVKYFEIVRLFITKFNLEPAGSHGMWGLDDYQFLPFLFGSSELCNTTLRFDELDGSKCYFVAVEKKLGGSSRILKSIMDKDWASINRGMIRMYDDHVLRRSVVTQHFIYGEYLRKDRSQANKDL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Can reactivate inactive phosphatase PP2A- phosphatase methylesterase complexes (PP2Ai) in presence of ATP and Mg(2+) by dissociating the inactive form from the complex .; PPIases accelerate the folding of proteins; Acts as a regulatory subunit for PP2A-like phosphatases modulating their activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a direct target of the PPIase.2. The protein should have peptidyl-prolyl cis-trans isomerase activity, phosphatase activator activity and be active in cytoplasm. The designed protein sequence is ",
    "response#1": "LRCSSL",
    "response#2": "ECKAGFAGDDAPRAVFPSIVGRPRH",
    "response#3": "MTRLSSTR"
  },
  {
    "reference": "MGGKWSKSWPQVRERIRRTPPAAEGVGAVSQDLDKHGAITSSNMNNADSVWLEAQEEEEVGFPVRPQVPLRPMTYKEAFDLSFFLKEKGGLEGLVYSKKRQEILDLWVYNTQGFFPDWQNYTPGPGIRYPLCFGWCFKLVPVNPEEVEEENKGENNCLLHPMSQHGMEDEDREVLMWKFDSSLARVHKARELHPEFYKDC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should contain at least one PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Dileucine internalization motif; necessary for CD4 internalization motif to achieve its intended function.2. The protein must exhibit the following characteristics: Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis.3. The designed protein should be involved in suppression by virus of host autophagy, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I by localizing to extracellular region, membrane, virion component, host cell Golgi membrane, host cell plasma membrane and exhibiting GTP binding, SH3 domain binding. The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPAVRERIRQTPPAAEGVGAVSQDLDNRGAITSSNMNSADSVCLQAQKEEDEVGFPVRPQVPLRPMTYKGAFDLSFFLKEKGGLDGLIYSKKRQDILDLWVYNTQGYFPDWQNYTPGPGIRYPLCFGWCFKLVPVDPREVEENNEGENNCLLHPMSQHGIEDEEREVLRWKFDSSLARRHIAREQHPEFYKDC",
    "response#2": "MGGKWSKRSVPGWNAVRERMRRAEPRTEPAAEGVGAASRDLAKHGALTTSNTVANNADCAWLEAQEDEEVGFPVRPQVPLRPMTYKAAVDLSFFLKEKGGLEGLIHSQKRQDILDLWVYNTQGFFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPEKVEEANEGEDNCLLHPMSQHGMEDEEKEVLVWRFDSTLAFHHRARELHPEFYKDC",
    "response#3": "MGGKWSKRSGWAAVRERMRRTEPAADGVGAASRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPEKIEEANEGENNCLLHPMSLHGMEDPEKEVLQWKFDSRLAFHHVARELHPEYYKN"
  },
  {
    "reference": "MDKSSASFESTRSFAEKMDSRDRLFHFRDKFFIPAHQGGGEVLYFTGNSLGLQPRKTREYIEQELKDWETLGVEGHFHAKNPWMPYHEFLTHSMAKIVGAKPIETVVMNSLTVNLHLLMVSFYRPTPTRYKIIIEGDAFPSDQYAVKSQIKFHQRKISTQSGSDAQETLTRNSVKNQNATATASDWLVELTPREGETILRTEDILKTIDNHDDSVALIMLGGVNYYTGQAFDMKRITEAGQKVGAVVGFDLAHAAGNLELKLHEWNVDFACWCSYKYLNSGPGGIAGIFVHERHADNPKLPRFTGWWGHDKETRFLMDDEFVPIKGAEGWQLSNPPIFQMASLRASLEIFEEAGMKALREKSKKLTGYMEFLLNEIADERISIITPSNPDERGCQLSIRVENSDKSLFDAITKKGVVADWREPDVIRVAPVPLYNSFMDVYRFVEILKDCLR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should be designed to catalyze the following reaction H2O + L-kynurenine = anthranilate + H(+) + L-alanine.2. The designed protein should have a high specificity for pyridoxal 5'-phosphate binding, with minimal off-target effects.3. pyridoxal 5'-phosphate is required for the proper folding and stability of the protein.4. For general function, the protein need meet that Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively.5. We require a protein that can specifically bind to the metabolites produced in the NAD(+) biosynthesis, Cofactor biosynthesis, quinolinate from L-kynurenine: step 2/3 pathway.6. The designed protein must possess pyridoxal phosphate binding, kynureninase activity to support 'de novo' NAD biosynthetic process from tryptophan, quinolinate biosynthetic process, anthranilate metabolic process, L-kynurenine catabolic process, tryptophan catabolic process under certain conditions. The designed protein sequence is ",
    "response#1": "LVGAKQNEVAVMGSLTSNLNALLIHFYKPKGKRTKILFEKQAFPSDYYAFLNIVQVFGYDASHLIQIEIPKGETYIKTETILDVFDKYEDEIAIVCLPGIQYYTGQFFDIAKITKHVKTSAPDVVVGWDLAHAVGNVPLSLHDWGVDFAAWCSYKYLNAGPGAIAGIFVNEKYTEQNKPENLILVGAKQNEVAVMGSLTSNLNALLIHFYKPKGKRTKILFEKQAFPSDYYAFLNIVQVFGYDASHLIQIEIPKGETYIKTETILDVFDKYEDEIAIVCLPGIQYYTGQFFDIAKITKHVKTSAPDVVVGWDLAHAVGNVPLSLHDWGVDFAAWCSYKYLNAGPGAIAGIFVNEKYTEQNKPENLFADDRAAEMIAPIVGAQTSEVAVMQTLTANLHLLLSAFYQPKADSRHKIILEKKAFPSDHFAVETQIRHHGLAVESSMVMIETLRSESTALTTQDVVAVIEEHAKDTAVLLLPGIQYYTGELLDIPTITELAHRHGIFVIWDLAHAVGNVPLRLHDWNVDAAVWCSYKYLNSGPGGIGGLFVHTQNSGVIRPIGEVEILVGAKQNEVAVMGSLTSNLNALLIHFYKPKGKRTKILFEKQAFPSDYYAFLNIVQVFGYDASHLIQIEIPKGETYIKTETILDVFDKYEDEIAIVCLPGIQYYTGQFFDIAKITKHVKTSAPDVVVGWDLAHAVGNVPLSLHDWGVDFAAWCSYKYLNAGPGAIAGIFVNEKYTEQNKPENLI",
    "response#2": "ADDRAAEMIAPIVGAQTSEVAVMQTLTANLHLLLSAFYQPKADSRHKIILEKKAFPSDHFAVETQIRHHGLAVESSMVMIETLRSESTALTTQDVVAVIEEHAKDTAVLLLPGIQYYTGELLDIPTITELAHRHGIFVIWDLAHAVGNVPLRLHDWNVDAAVWCSYKYLNSGPGGIGGLFVHTQNSGVIRPGPELDPLADDRAAEMIAPIVGAQTSEVAVMQTLTANLHLLLSAFYQPKADSRHKIILEKKAFPSDHFAVETQIRHHGLAVESSMVMIETLRSESTALTTQDVVAVIEEHAKDTAVLLLPGIQYYTGELLDIPTITELAHRHGIFVIWDLAHAVGNVPLRLHDWNVDAAVWCSYKYLNSGPGGIGGLFVHTQNSGVIRPGDVSLADDRAAEMIAPIVGAQTSEVAVMQTLTANLHLLLSAFYQPKADSRHKIILEKKAFPSDHFAVETQIRHHGLAVESSMVMIETLRSESTALTTQDVVAVIEEHAKDTAVLLLPGIQYYTGELLDIPTITELAHRHGIFVIWDLAHAVGNVPLRLHDWNVDAAVWCSYKYLNSGPGGIGGLFVHTQNSGVIRPGDVSLDPFHKKPKLPLDFEKPDKTERTAWSYRNWLGRTNYVLDFTQRILRY",
    "response#3": "LVGAKQNEVAVMGSLTSNLNALLIHFYKPKGKRTKILFEKQAFPSDYYAFLNIVQVFGYDASHLIQIEIPKGETYIKTETILDVFDKYEDEIAIVCLPGIQYYTGQFFDIAKITKHVKTSAPDVVVGWDLAHAVGNVPLSLHDWGVDFAAWCSYKYLNAGPGAIAGIFVNEKYTEQNKPENKNICKVIYADDRAAEMIAPIVGAQTSEVAVMQTLTANLHLLLSAFYQPKADSRHKIILEKKAFPSDHFAVETQIRHHGLAVESSMVMIETLRSESTALTTQDVVAVIEEHAKDTAVLLLPGIQYYTGELLDIPTITELAHRHGIFVIWDLAHAVGNVPLRLHDWNVDAAVWCSYKYLNSGPGGIGGLFVHTQNSGVIRPLSYAIMNADDRAAEMIAPIVGAQTSEVAVMQTLTANLHLLLSAFYQPKADSRHKIILEKKAFPSDHFAVETQIRHHGLAVESSMVMIETLRSESTALTTQDVVAVIEEHAKDTAVLLLPGIQYYTGELLDIPTITELAHRHGIFVIWDLAHAVGNVPLRLHDWNVDAAVWCSYKYLNSGPGGIGGLFVHTQNSGVIRPSYYIP"
  },
  {
    "reference": "MPIYDEASVPGTAAGRSTTDVGATAGANPFNIPADEEIFRFREEERARKEQDKLIAQTMRVADKTTFAAQMQATATADARTLLRELRPPKGPKATTTLAASSVGTLDRRKEKENMADFIAKKREIFLLQMSLDTKRAEIKKLEERARQREEALKKSEQMLEEDALRFDAFLKENDEKVQEAIKKAEAEAKAKQDKVLEIKRLNTATAALRSELNKYEEQLEDCRRYKEFLDSITPPEWFEQQAAKLQRRKDALVAEWQSQCEALKQRREAALAAKTAAESDYANARTQQQAERAERAIKESVAALKEIMKEKEPQPPNLDFEMDPEDEEMYFQEPGQLLAVYKQLEESNLFYIQNAQETEEALEELRQKLRDTKTRMDAEAQGLQGQVSTLQASIVAAREKAKRLKDRTLENEGAFTLSMGSSNAPTSSVTGSSGPGGPVNLKELGDKVREVYVRCGFDADASISTLQMLTNIEMKLEEYLNLAEGMTPDYVDGAEKAREKDRRKVARDEKLSTQHREHEARMARALERAAAPVFKKTGKPLMFRSAPPQRKKVVQADDRNDEEAELEAYLAQDMI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Design the protein with a signal peptide incorporated.2. For general function, the protein need meet that As part of MIA, a complex associated with the outer doublet microtubules of the axoneme, may play a role in ciliary/flagellar motility by regulating the assembly and the activity of axonemal inner dynein arm.3. The designed protein should have dynein complex binding within motile cilium, axonemal outer doublet to regulate inner dynein arm assembly, cell motility, cilium movement. The designed protein sequence is ",
    "response#1": "YYPAGVQRPGLGCRELIFRNLSKILPAISRDVTNWVAGTRIKSSQLLAVLLLHGEDHITQHMELLLSTLYHSCLDEENQVVINSVKSAELIGTFVNPEVFLKLILSALQKSPLASHLMTLAACIRGCSREMLKTHLTKITSVISQSDVCQGSERVLYQEQLLCCIQSLIDVCQQDCTEVSLQCMNVLITILV",
    "response#2": "YYPAGVQRPGLGCRELIFRNLSKILPAISRDVTNWVAGTRIKSSQLLAVLLLHGEDHITQHMELLLSTLYHSCLDEENQVVINSVKSAELIGTFVNPEVFLKLILSALQKSPLASHLMTLAACIRGCSREMLKTHLTKITSVISQSDVCQGSERVLYQEQLLCCIQSLIDVCQQDCTEVSLQCMNVLITILS",
    "response#3": "YYPAGVQRPGLGCRELIFRNLSKILPAISRDVTNWVAGTRIKSSQLLAVLLLHGEDHITQHMELLLSTLYHSCLDEENQVVINSVKSAELIGTFVNPEVFLKLILSALQKSPLASHLMTLAACIRGCSREMLKTHLTKITSVISQSDVCQGSERVLYQEQLLCCIQSLIDVCQQDCTEVSLQCMNVLITILF"
  },
  {
    "reference": "MYKKIPELQQLPYALYNATQVQAFDKCIIEEYNISGTTLMERAGAAAWNAAYQRWPAIKNVTVLCGTGNNGGDGYVVARLALDSGCVVQLLQLGNINHLTDTAKIMLQRYQAAGGTVQPFEDLPRKTDLIVDAMLGTGLTRDVTGIWANAITAINSQHAPILALDIPSGLNANTGHIMGCAVRAQLTVTFIALKQGLFTGYGLECCGKIEFNGLQAPAAIYSREILNARRIDWQQQASTLCPRPRNAHKGNFGHVLIIGGAAGMNGALRLAGAAAARCGAGIVTLAAAPGQGALLNLAIPELLCYEVANVQDLEPLLARATVIAIGPGLGKSNWAQQLLNRVLNAPQSLVVDADALNLLAVSDTAIQRSDWVLTPHPKEAARLLKCSTAEIQEDRFLALQRLVTNYGGTLVLKGSGTLIGTDSHKNPAICSAGNPGMATAGMGDLLTGIIAGFIAQGWSPKDAAYLGVCLHAAAGDLAAYDGERGLLASDLLPYLRKLLNPRPERC",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The desired protein should be able to perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate2. The protein requires Mg(2+) to activate a critical catalytic site.3. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration4. The protein must be able to ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, metal ion binding, kinase activity.5. The designed protein should be able to compete with endogenous proteins or inhibitors for K(+) binding.6. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MMKLENAADQSGVTYDQMMENAGKGLAEHLVRIAVEMGVKEILFLCGNGNNAGDCFTAARLLTPQFKVTACLLAGVPKTRTAYAKYKMLKDIAVLEDQEAILEAVRRHRLIADGVFGIGFRGELSPFVQSVFAEIEADSEKKCIAVDIPSGGNGLSGDVAKGTPHCDMTITFGAAKTGLFLTPLSEYCGAIMLVDIGIPAPAFTPAFVKTILKPRQKYAHKGTYGHALIIGGSYGKLGALILAAKAALRAGVGLLTVQVPQVGYSIVQTAVPEAMALTDAHDLHFTRFTGQPDAYACLGIGPGLGQAEDTRLALEVLFRHHLPPLVLDADALNLLAADRTLLRTLPEGSILTPHLKEFERLAGPATDDFHRLQLLQSFCHEHKCYVVLKGAHSCVGTPTGEFYFNSTGNPGMATGGSGDVLTGILTSLRAQGYTALETCLLGVYLHGLAGDLAAQEMGQASLVASDLTAFLGKAFLASNTDIPELLPPRPSQGHKGMFGRLMCVSGSRNMPGAAILASKAALRCGVGLFCLASDIDVCRLLIAQAPEATMLPLPTDYDGKLTQQAVPAILDYAKQATCVLIGCGLGQSEALRQAVTSLIPLLECPIILDADGLNAIAPSIDILHKAKSTVVLTPHPGEMARLLHCSVEEVQKDRMTAAKRLATRFPNTVVVLKGAGTIVATADHAHINTTGNSGMSKGGSGDILAGMIASLTAQHISPENAAIAGVYLHGLAGDCAAAENSRRAMLPSDMISQLPMLF",
    "response#2": "MMKLENAADQSGVTYDQMMENAGKGLAEHLVRIAVEMGVKEILFLCGNGNNAGDCFTAARLLTPQFKVTACLLAGVPKTRTAYAKYKMLKDIAVLEDQEAILEAVRRHRLIADGVFGIGFRGELSPFVQSVFAEIEADSEKKCIAVDIPSGGNGLSGDVAKGTPHCDMTITFGAAKTGLFLTPLSEYCGAIMLVDIGIPAPAFVSGSESCIGAAWMSTNAALRTGSGLVTLASVSEVTTSVATSLHECIYLPLGSKTLTSDCADKLCKNARTATAILFGCGVGNSDEAYRLLCALIDNTSCPIVIDADGINSLAPHINELKDNKGRLILTPHIKEFSRLSGLDTDYILRHKLSCAKDFAVKYGVHILLKDAYSVYASPDGFAAVNMSGNAALAKGGSGDTLAGTIGGLLAQGIETGNAVRLGAYLFGLSAQYAARERSMSGILPSEVLCVGGEEGMGGAVRLCAEGALRTGVGLASVATRRSGVAALVAARPEAMTHAVEDAAALRPLVERATVLAVGPGLGQGDWGRALFAEALGSGKPLVLDADALNLLANHPVAVPQAILTPHPGEAARLLACDKGAVQADRFAAAEALAQKFQCTVVLKGAGTIVAAPSEIPAVIGAGNPGMATGGMGDVLSGVIGALVAQGLPLFQAAVAGALLHSAAGDAAARVGGERGLLPSDLE",
    "response#3": "MMKLENAADQSGVTYDQMMENAGKGLAEHLVRIAVEMGVKEILFLCGNGNNAGDCFTAARLLTPQFKVTACLLAGVPKTRTAYAKYKMLKDIAVLEDQEAILEAVRRHRLIADGVFGIGFRGELSPFVQSVFAEIEADSEKKCIAVDIPSGGNGLSGDVAKGTPHCDMTITFGAAKTGLFLTPLSEYCGAIMLVDIGIPAPAFDKYTQGVTGVIAGSATYPGAAVLCTGAAVAATSGMVRYAGTATAQVLAAWPEVIAAPSAEASGRVQAWAVGPGMGTDTAAADELRFALGTDLPVIVDADGLTLLAADPNLVADRDAPTVLTPHAGEFARLAAGVGMEVLDDRVTAARELADRYRAVVLLKGHVTVIADPAGPVYLNPAGGSWAATAGSGDVLSGIIGALLAAGIPPAEAAAMAAFVHARAANLSAADPRVMQKAAEACYNTIINTISTQKILVICGPGNNGGDGILITKHLHDKQKNVSLYAPLGIAKTDDSKKALTLLNNNALIKQNVNINNFEIIIDSIFGIGLNKPITEELSNLFRSINKTKSKVISIDMPSGVHTDTGRVDSIAVKADITLTFHRLKPGQFLLPGKEYVGEIKLLDIQELYKNKAGRVAVIAGSIGMLGAAQLCTEAALRAGVGLVVLYCLPETYPLLAARVSPEIMVKQVQTYKNIDEDDAQALLIGPGLGQLDNANQLALHALVSCFDGTVVLDADGINLAAERSWRFEKNHILTPHPGEMRRLLPSAGMNTPRKELVNSFLYNHDATLVLKGARTIIGQRRQGMLYNSTGGPSMAKGGQGDVLAGICAGLAAQGMMPRDAAALAVYLCGRASELAVSR"
  },
  {
    "reference": "MRVRGILRNCPQWWIWGILGFWMIIICRVAGNLWVTVYYGVPVWKEAKTTLFCASDAKAYDREVHNVWATHACVPTDPNPQELVLENVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCVNITNSTTSNGDGNATHSDSIKEEIKNCSFNTTTEIRDRKQNVHALFYRSDVVPLNEENGTNNNYILINCNTSTITQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIIRSENLTDNVKTIIVQLNESVVINCTRPGNNTRKSIRIGPGQTFFATNIIGDIRQAHCNISRKAWNTTLERVNKTLGKHFPNKTIIFRPHSGGDLEITTHSFNCRGEFFYCNTSKLFNGNTSKLFNGTGNSSEVITLPCRIKQIINMWQKVGRAMYAPPIEGNITCSSSITGLLLTRDGGTRNDTESDGGTRNDTEIFRPGGGNMKDNWRSELYKYKVVEVKPLGIAPTGAKRRVVEREKRAVGLGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGLWGCSGKLICTTAVPWNSSWSNKSQTDIWNNMTWMQWDREISNYTGIIYSLLEDSQSQQEKNEKDLLALDSWKNLWSWFDISNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTLTPNPREPDRLGRIEEEGGEQDKDRSVRLVSGFLALAWDDLRSL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Transmembrane protein gp41: Acts as a class I viral fusion protein2. The protein contains novel Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains that confer a unique function or activity.3. The designed protein should be involved in virion attachment to host cell, fusion of virus membrane with host plasma membrane, positive regulation of receptor clustering, clathrin-dependent endocytosis of virus by host cell, positive regulation of plasma membrane raft polarization by localizing to plasma membrane, host cell plasma membrane, host cell endosome membrane, viral envelope and exhibiting structural molecule activity.4. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "MRVMGIRRNYQHLWRWGTMLLGMLMICSATEQLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPADPNPQELELKNVTENFNMWKNDMVNQMHEDVISLWDQSLKPCVKLTPLCVTLNCSNVSSNSSGTSSNMTXNXELWVTVYYGVPVWKEATTTLFCASDAKAYDREAHNVWATHACVPTDPHPQEVRLGNVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVTLKCNNTANNSNSNTTAGNSSVEGKIKGEMKNCSFNITTSIKDRVQKEYALFYNLDIVPIDNDNDNTSYRLINCNTSVITQACPKVTFEPIPIHYCAPAGFAILRCNNKTFSGKGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSDNFTDNAKTIIVQLKEAVNITCTRPGNNTRRSIPMGPGRAFYTTGDIIGDIRQAHCNLSRTSWNNALNQTAIKLREQFNNKTIIFNQSSGGDLEIVMHSFNCGGEFFYCNVTELFNSTWNGTEQNSTQNEDTITLPCRIKQIINMWQEVGKAMYAPPIRGNITCSSNITGLLLTRDGGNNESTNETFRPGGGDMRDNWRSELYKYKVVKLEPLGVAPTKAKRRVVQREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLALEXYLQDQQLLGLWGCSGKLICTTNVPWNSSWSNRSETDIWQNMTWMQWEREIDNYTDTIYKLLEVSQNQQEQNEKDLLALDSWNSLWSWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSIMNRVRQGYSPLSFQTL",
    "response#2": "MRVMGTQRNWPPWWIWGILGFWMLMICSVAGNLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPADPNPQELELKNVTENFNMWKNDMVNQMHEDVISLWDQSLKPCVKLTPLCVTLNCSNVSSNSSGTSSSINSSNETMKNCSFNTTTELRDKKKQTYSLFYVDDLTQINKTTESYKLINCNTTAITQACPKISFEPIPIHYCAPAGFALLRCNNKTYNGSGPCNNVSTVHCTHGIKPVISTQLILNGSLAEEKIMIRYKADKVLVQLNTSIHINCTRVGNKTIKGIPLGPGQLFYGTETVVGDTREAHCEINQTHWYKILNQVKRELTTVFNESNKTVHFANSSGGDPEVANLHFNCGGEFFYCNTSALFNDTLLRNKTLLNETLHNNLTLPCRIRQIVNLWLRVGKGIFAPPIRGNIRCNSTITGLILEKHTNTDNITFRPIGGDMTDIWRSELYNYKIVKIEPLGVAPTRARRRTVDREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLALEXYLQDQQLLGLWGCSGKLICTTNVPWNSSWSNRSETDIWQNMTWMQWEREIDNYTDTIYKLLEVSQNQQEQNEKDLLALDSWNSLWSWFDITNWLWYIKIFIMIVGGLIGLRIIFAVLSIMNRVRQGYSPLSFQTLTHHQREPDRPGRI",
    "response#3": "MRVKGTRKNYQHLWKWGTMLLGMLMICSAVEQLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPADPNPQELELKNVTENFNMWKNDMVNQMHEDVISLWDQSLKPCVKLTPLCVTLNCSNVSSNSSGTSEDMXXEVKEAMKNCSFNITTELRDKSQXTYSLFYRLDITPIXDNSSEYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEDIIIRSENITNNAKTIIVQLDSPVNISCIRPGNNTRKSIRIGPGQTFFATGDIVGDIRQAHCNVSRSXWNETLXKVAKKLMGLXNKTXILLNPSSGGDPEITMHSFNCGGEFFYCNTSGLFNSTWNNXTGSXQGSNGTGLNDTITLQCRIKQIINMWQGVGKAMYAPPIAGNITCLSNITGLLLTRDGGANSSHNETFRPGGGDMRENWRSELYKYKVVRLEPLGLAPTRARRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASITLTAQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSNKSQAEIWNNMTWMQWDREISNYTNTIYKLLEESQDQQEKNEKDLLALDNWNNLWNWFDITKWLWYIKIFIIIIGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTLLP"
  },
  {
    "reference": "MNIFEELKARGLVFQTTDEEALVKALTEGQVSYYTGYDPTADSLHLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSGKIKGQLSQFLDFENGANKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIETGISYTEFAYQIMQGYDFYELNQKFNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVMTVPLITDANGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDEDAIRFLKIFTFLSLEEIAEIEKAFDAARHERLAQKTLAKEVVTLVHGEEAFKQALNITEQLFAGNIKNLSAAELKQGLSNVPNYQVQDEDNLNIVEILVSSSISPSKRQAREDVQNGAIYLNGERIQDLDYTLTEDDKIDGQLTVIRRGKKKYAVLTY",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The ATP, L-tyrosine binding site should be located in a region of the protein that is accessible to the ligand.2. The designed protein should have tyrosine-tRNA ligase activity, RNA binding to facilitate tyrosyl-tRNA aminoacylation.3. The presence of S4 RNA-binding domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MALNSLPNTQINKDTKVIDALVELKVASSKREAREFLKNKAILINNQVIEDENLVISSFDLIQNKYLLVKKGKKKYFVN",
    "response#2": "MALNSLPNTQINKDTKVIDALVELKVASSKREAREFLKNKAILINNQVIEDENLVISSFDLIQNKYLLVKKGKKKYFVK",
    "response#3": "MALNSLPNTQINKDTKVIDALVELKVASSKREAREFLKNKAILINNQVIEDENLVISSFDLIQNKYLLVKKGKKKYFVK"
  },
  {
    "reference": "MKLPPVFVFELVENQGLANIALIRPRVIAPDNNLRPGGIVSGIAGLLTLGQENRNLISENRQVINNNTTAIGQNSDRIDANAKGVADNRAAIGQNSGRIDANAKGVADNKAAIGRNSGRIDANAKGVADNKTAIGRNSGRIDTNAKGVADNRAAISQNRGRINANAAGVASNRAAIRQNSAAISALGQRVDGLQGQINSARKEARAGAANAAALSGLRYDNRPGKVSIATGVGGFKGSTALAAGIGYTSKNENARYNVSVAYNEAGTSWNAGASFTLN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in cell surface, cell outer membrane that is critical for cell adhesion, protein transport.2. For general function, the protein need meet that Also implicated in the resistance to porcine serum.; Involved in adhesion to host epithelial cells and is required for full virulence in mice; Participates in bacterial attachment to several surfaces, including various extracellular matrix (ECM) components and a hydrophobic abiotic surface3. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "NEKNSIGSAFTETVSHVGNKENPGYYKQTIYVNPSENSLTNAKLKVQAYHSSYPNNIGQINKDVTDIKIYQVPKGYTLNKGYDVNTKELTDVTNQYLQKITYGDNNSAVIDFGNADSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTC",
    "response#2": "NQKGQQLISSTNYINNEDLSRNMTVYVNQPKKTYTKETFVTNLTGYKFNPDAKNFKIYEVTDQNQFVDSFTPDTSKLKDVTGQFDVIYSNDNKTATVDLLNGQSSSDKQYIIQQVAYPDNSSTDNGKIDYTLETQNGKSSWSNSYSNVC",
    "response#3": "GLLSEGVLGILGNLPLLDILKAGGNTPSGLLGGLLGKLSSTIPLLNDIVDLQITDPQLLELGLVQSPDGHRLYVTIPLSLVLNVKTSVVGSLLKLAVKLNITVELLAVKDEQGKSHLVLGDCTHSPGSLKISLLDGLGPLVPQDLLDSITGVLDNVLPGLVQGEVCPLVNEVLSHL"
  },
  {
    "reference": "MSKLFNIAIVGATGAVGEALVEVLEQREFPVGKLFLLASDRSLGKVIYFRERSVKVQELEKFDFAQADIAFFTAGSDVSKTYAPKATAAGAVVIDNTSAFRLDADVPLVVPEVNPEAVAEFKHRKIIANPNCSTIQMLVALKPIYDAVGISHIDVATYQAVSGAGREAVEELVKQTIHMMSGKGREDAPCSVLPTTIGFNVLPHIDVLLDDGYSGEEMKMVNETKKILGDSTVIVNPTCVRVPVIFGHSEAVHVRTKAAISLEQARELLASAPGVSLMNDGYPTPANEAAGNDQVWVGRLRQRTGDDLGLNLWVVSDNVRKGAATNSVQIAELLISSYL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. We are looking for a protein with broad substrate specificity for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.2. A protein should be able to manipulate the activity of enzymes in the L-threonine biosynthesis pathway, either by activating or inhibiting their function.3. The designed protein should be optimized for aspartate-semialdehyde dehydrogenase activity activity in order to improve 'de novo' L-methionine biosynthetic process. The designed protein sequence is ",
    "response#1": "LKPLMKFGIEKIYLASMQAISGAGYDGVASMAILDNVIPYIDKEEEKVETETKKLLGEFNGSEIVDAPFAVSASCNRVMVMDGHTEAVWVEMADNPSTEDIKQAFLEFDPGLSDLPTEPAPVIEVKEEKDRPQPRMDRNLGGGMTVSVGRIRPGIRYICMGHNR",
    "response#2": "LFPLAKRYGLKRVVVTTFQSVSGSGKQAVEQLRAERKGELNTGSNVYPHPIFNNCLPHIGNFLENGCTDEEIKMVNESRKILDLPDLPITATTVRVPVEYSHSVSLNVELEANFNIEDVRNLYRHTAGIILKDAPAQNIYPLPRDAMGRDDVFIGRLRRDESVPFGLNMWVVADNLREA",
    "response#3": "LKPLMKFGIEKIYLASMQAISGAGYDGVASMAILDNVIPYIDKEEEKVETETKKLLGEFNGSEIVDAPFAVSASCNRVMVMDGHTEAVWVEMADNPSTEDIKQAFLEFDPGLSDLPTEPAPVIEVKEEKDRPQPRMDRNLGGGMTVSVGRIRPGIRYICMGHNK"
  },
  {
    "reference": "MRKGKCDVAVAGATGAVGREMINMLSANNFPVNELRLLASSRSAGQKLKFKESEIVVEELSPEWIKKNRVDIVLMSAGGAVSLEYAPLFSEKKIYVIDNSSAWRMNPAVPLVVPEINPHDLAVDKYIIANPNCSTIQMVHALAPVHRISKIKRIIVVTFQAVSGAGWKAVKELESQSKKIADGDFRELNLPKNKISVFPHQIAFNCIPQIDVFLENFFTKEEMKMVQETKKILSSEIEVSATCVRVPVFRGHSEAVNVELSDDITKDEIWEALLKEKNISLIDDVSKLRYPMAIDAAGKSATFVGRIRHDESRPKCWWFWIVSDNLLKGAALNAVQIAEELIKII",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should take into account the physiological conditions of the Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5, L-threonine biosynthesis pathway.2. The protein should have a high affinity for phosphate, NADP(+), substrate.3. The designed protein must have at least one Semialdehyde dehydrogenase NAD-binding domain.4. The designed protein should be optimized for aspartate-semialdehyde dehydrogenase activity, protein dimerization activity, NADP binding, NAD binding activity in order to improve diaminopimelate biosynthetic process, isoleucine biosynthetic process, 'de novo' L-methionine biosynthetic process.5. The designed protein must have a Acyl-thioester intermediate, Proton acceptor active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "MRIAILGATGAVGKELLELLESRNFPLSDLRLLASQRSVGRKLQFKGEDLPVEVVSDRAFNNVDLVLASAGGATSKAWASIAVNAGAVVIDNSSAYRMNPEVPLVIPEVNPQAAANRPRGIIANPNCTTLSMMAALKVLHDAAGLVRLNISSYQAVSGSGLAGVEALVKQTDHIGEHNIDLVHSETDNTRVNSDDLGPYADPIVYNALPLAGALVDDGSGETDEEQKLRNESRKILGLPDLRVSGTCVRVPVFTGHTLTIHAEFARDITPEQAQALLADAPGVKLVDVPTPRAAAGVDESLVGRIRQDQSLDNNRGLVLVVSGDNLRKGAALNTYEIAEALLAPLHEAFGVKRAIVSTYQAASGAGAEGMTELEQGARQYLAGEPVTASKFAHPLAFNLIPHIDSFQDNGYTREEMKVLWETRKIMEAPEVLLSCTAVRVPTMRAHAEAVTIETRHPVTPAAAREVLAKAQGVTLADDPSNKLYPMPLTASSKYDVEVGRIRESLVFGDTGLDFFVCGDQLLPLPD",
    "response#2": "NVAVVGATGLVGSEFLKSLRSEYVKELLPIASENSKGKRIMFNQKEYDILTINEGLESKIDVAFFCAGSQISKNYAREFAKKGAIVIDNSSYFRMNDEIPLVVPEVNGEVLDEALAKGGRNWIGGNCTVSCMLMGLAPLHKKYTMRRVIVSTYQSVSGTGVKAVQQLDNEIAGIKGEMAYPYPINRNALPHCDVFEENGYTKEEMKLAREPQKILNDKTFSVSATAVRIPTAGGHSESVNVEFENDFDLAQVRKLLHETPGIVVQDNPATNTYPMPIYAHDKDDVFVGRIRRDETQPNTLNMWIVSDNLRWGAAEPLRRMLRILLE",
    "response#3": "TLAVVGATGAVGTVMLDLLSTREDVYGEIRLVASPRSAGKKLTVRGEQVEVVALAPEVFDGVDVAMFDTPDEVSKQWAPIAAERGAVAVDNSGAFRMDPDVPLVVPEVNAEAARNAPAGIIANPNCTTMAAMPVLAPLHWKYKIKRVVVSTYQSVTGSGIKGVKQLENERKNIEGEQAYPHPIDLNLIPQGGRFDGSGYTSEEVKLVDETRKILGAPEIQVTATVVRVPVYGGHSESVNIAFENDFDLEEVKQILNLMPGIKLQDHPENAIYPMPKFAEEKDDVFVGRVRRDFSNPNSLNLWIVSDNLRKGAALNAIQIAELVAAEL"
  },
  {
    "reference": "MSMRLTFKGDKKIQKKKKKNTKSLQSSLESAENDPFWTDANELNELEGPAVIYKIDDDAGVSLGIEEVKESCVLLPLDKVSLEPELTRQVFLLSILNNTILLKSCLGKYMSCSKSGDLYCTQEAVGSQEQWIAENLGSGFWAWKSVSTKKYLTLSREKQDQAIACVSDTVIPEAKWRIRVQTRFLKKNKSSLFDNPTIHSRQLESMAGRKLSTDEKKTLKKAFKEGVLHEALLDLRVSSRSDKYG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that contributes to mRNA cis splicing, via spliceosome by functioning in catalytic step 2 spliceosome, nucleus, nucleolus with actin filament binding.2. For general function, the protein need meet that May have a role in processing of pre-rRNA or in the assembly of rRNA into ribosomal subunits; May be involved in pre-mRNA splicing . The designed protein sequence is ",
    "response#1": "MEQSLFTRFVRVGVPTVDLDAQGRARASLCNLYNWRYKNLGNLPHVQLLPEFSTANAGLLYDFQLINVEDFQGVGESEPNPYFYQNLGEAEYVVALFMYMCLLGYPADKISILTTYNGQKHLIRDIINRRCGNNPLIGRPNKVTTVDRFQGQQNDYILLSLVRTRAVGHLRDVRRLVVAMSRARLGLYIFS",
    "response#2": "MEQSLFTRFVRVGVPTVDLDAQGRARASLCNLYNWRYKNLGNLPHVQLLPEFSTANAGLLYDFQLINVEDFQGVGESEPNPYFYQNLGEAEYVVALFMYMCLLGYPADKISILTTYNGQKHLIRDIINRRCGNNPLIGRPNKVTTVDRFQGQQNDYILLSLVRTRAVGHLRDVRRLVVAMSRARLGLYIFP",
    "response#3": "MEQSLFTRFVRVGVPTVDLDAQGRARASLCNLYNWRYKNLGNLPHVQLLPEFSTANAGLLYDFQLINVEDFQGVGESEPNPYFYQNLGEAEYVVALFMYMCLLGYPADKISILTTYNGQKHLIRDIINRRCGNNPLIGRPNKVTTVDRFQGQQNDYILLSLVRTRAVGHLRDVRRLVVAMSRARLGLYIFS"
  },
  {
    "reference": "MSYNITNPNQYQYFAAVWAEPIPMLNQCMSALSQSYQTQAARDTVRQQFSNLLSAVVTPSQRFPDTGSRVYVNSAVIKPLYEALMKSFDTRNRIIETEEESRPSASEVANATQRVDDATVAIRSQIQLLLSELSNGHGYMNRAEFEALLPWTTAPAT",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Capsid protein self-assembles to form rod-shaped virions about 18 nm in diameter with a central canal enclosing the viral genomic RNA..2. The protein can be expressed in helical viral capsid and perform structural molecule activity without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "NDTNDQSFNDYLCAANMLYPIPANATSVPISIPSRNWAAFRGWSFTRLKTKETPSLGSGFDPYFVYSGTIPYLDGTFYLNHTFKKVSIMFDSSVSWPGNDRLLTPNEFEIKRSVDGEGYNVAQCNMTKDWFLIQMLSHYNIGYQGFYIPESYKDRMYSFFRNFQPG",
    "response#2": "NDTNDQSFNDYLCAANMLYPIPANATSVPISIPSRNWAAFRGWSFTRLKTKETPSLGSGFDPYFVYSGTIPYLDGTFYLNHTFKKVSIMFDSSVSWPGNDRLLTPNEFEIKRSVDGEGYNVAQCNMTKDWFLIQMLSHYNIGYQGFYIPESYKDRMYSFFRNFQPC",
    "response#3": "DNNEGADGVGNSSGNWHCDSTWMGDRVITTSTRTWALPTYNNHLYKQISSQSGASNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRNWLPGPCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQGVLPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYNKSE"
  },
  {
    "reference": "MSERVILAYSGGLDTSVAISWIGKETGREVVAVAIDLGQGGEHMDVIRQRALDCGAVEAVVVDARDEFAEGYCLPTVLNNALYMDRYPLVSAISRPLIVKHLVAAAREHGGGIVAHGCTGKGNDQVRFEVGFASLAPDLEVLAPVRDYAWTREKAIAFAEENAIPINVTKRSPFSIDQNVWGRAVETGFLEHLWNAPTKDIYAYTEDPTINWGVPDEVIVGFERGVPVSVDGKPVSMLAAIEELNRRAGAQGVGRLDVVEDRLVGIKSREIYEAPGAMVLITAHTELEHVTLERELGRFKRQTDQRWAELVYDGLWYSPLKAALEAFVAKTQEHVSGEVRLVLHGGHIAVNGRRSAESLYDFNLATYDEGDSFDQSAARGFVYVHGLSSKLAARRDLR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP, L-citrulline binding.2. A protein should be able to manipulate the activity of enzymes in the Amino-acid biosynthesis, L-arginine biosynthesis pathway, either by activating or inhibiting their function.3. The protein should have a low Km value for ATP + L-aspartate + L-citrulline = 2-(N(omega)-L-   arginino)succinate + AMP + diphosphate + H(+).4. The designed protein should have ATP binding within plasma membrane, cytoplasm, cytosol to regulate arginine biosynthetic process, urea cycle. The designed protein sequence is ",
    "response#1": "YSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEYVEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETPGGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQDNVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKV",
    "response#2": "FSVDANLLHTSSEGKALENPADEAPDYVYQRTVSPEDAPDQPEYIEVSFERGDAVAINGEALSPATILTALNEYGRKHGIGRLDFVENRFVGMKSRGIYETPGGTILLEAHRGIEQITLDSGAGHLKDSIMPRYAELIYNGFWYSPEREMLQALIDKSQEHVTGTVRLKLYKGGVHTVGRWSEHSLYSEKHVTFEDDAGAYDQKDAAGFIRLNALRLKYSIDENLWGRSIEGDIISDPASEVPEDAFEWTAVRKQDKLKLSVEFEKGVPVRVNGEKLDPVKLISLLNEEVGSRGFGRVEHLENRVVGFKSREVYEAPAALALIAAHKDLEKTVLTPLELRFKRHLDSLWSDLVYQGLWYEPLRNTLELAGDEINKWVSGEVKLEVDLKSLRVVGRTSPYSPYSEKISSYNKGWYPSDEEARGFIEIWGMHCL",
    "response#3": "YSIDRNILGRSIEAGPLEDPMTEPTEEIYLMTKAIADTPDEPEYVDIGFEKGIPVSLNGVMLDPVTLVERLNEIAGNHGVGRLDMVENRVVGIKSREIYEAPALLVLIDAHRDLESLTQTADVTHYKNTVEEIYSQLIYRGLWYSPLKEALDAFIVKTQERVTGMVRVKFFKGNANVAGRKSDYSIYDAELATYGMEDQFDHKAAEGFIYIWGLPTKVYSIDQNVFGRAVETGFLEDIWNAPIEDIYEYTADPAVPREADEVVISFKEGVPVAVDGRPVTVLQAIQQLNERAGAQGVGRIDMVEDRLVGIKSREVYEAPGAIALITAHQELENVTVERELARYKRQVEQRWGEMVYDGLWFSPLKRALDGFINEANQHVTGDIRMTLHAGRAVVTGRKSEESLYDFNLATYDSGDTFDQSKAQGFIEIFGLSAKYSIDENLWGRSIEGDIISDPASEVPEDAFEWTAVRKQDKLKLSVEFEKGVPVRVNGEKLDPVKLISLLNEEVGSRGFGRVEHLENRVVGFKSREVYEAPAALALIAAHKDLEKTVLTPLELRFKRHLDSLWSDLVYQGLWYEPLRNTLELAGDEINKWVSGEVKLEVDLKSLRVVGRTSPYSPYSEKISSYNKGWYPSDEEARGFIEIWGMHR"
  },
  {
    "reference": "MALLQIAEPGQSAAPHQHRLAVGIDLGTTNSLVAAVRSGVADTLADESGRHALASVVRYSEDGVQVGVDAERFSAQDPLNTVVSVKRFMGRSLSDIQAKDASLPYNFSASENGLPLFNTQAGQFNPIQISADILRPLVDRAEKTLGGSLEGVVITVPAYFDDAQRQGTKEAASLLGVKVLRLLNEPTAAAIAYGLDSGQEGVIAIYDLGGGTFDISILRLNKGVFEVLATGGDSALGGDDFDHALAAHLQESWQLTSLTASERRALLIESRRVKEALTEQASVTASLTLEQGIVHEQVVDKAQFDALIESLVKKTIASCRRALRDAAISNDEVLETVMVGGSTRVPLVRERVEGFFGKAPLTSIDPDRVVAIGAAIQADILVGNKPESDLLLLDVIPLSLGIETMGGLVEKVVSRNTTIPVARAQEFTTFKDGQTAMAFHVVQGERELVADCRSLARFTLKGIPPMAAGAAHIRVTFQVDADGLLSVTAMEKSSGVQASIQVKPSFGLTDEEIGTMLKDSMAHAKEDIERRMLAEQQVEAARVLESLSAALAKDGDLLDEAEAATIQAGMANLAQVASQSDTDAIEKAIAALDDASQDFAAKRMDNSIRLALKGQSVDNI",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein must possess unfolded protein binding, ATP binding to support iron-sulfur cluster assembly under certain conditions.2. For general function, the protein need meet that Chaperone involved in the maturation of iron-sulfur cluster- containing proteins The designed protein sequence is ",
    "response#1": "FILQMSPQARASLAAYYNLSVQEVQLWIQSVFTNELKFNVVVDTGFSREISLRQAAIEFCQSWVAANAAKTVYNSKSGEVAPKPLPVLSSSCPGWICYVEKTHSSLIPHISTVRSPQQVAGRLLKDWFSYQLGISRKKIWVLSLMPCFDKKLEASRNDFVNEQVRDVDCVITPKELVELLKTKNISPQSMDLDSLSISEQTPCLPSWYEPVQFEQQNGSSSGGYLHYIMTFAAKALFDINDLTNRINVSQKNADMIEYELTSPDTGETLLRMATCYGFRNIQNLVRNVGRKSAPRRGRVLLKRMKNMSTSPSTGTGKQKLDYVEVMACPGGCINGGE",
    "response#2": "VFVASISHQSRASLATAYNLSIEEIDKLLINLFINQMGFKYIVGTSIGRKLSLINEAQNLIEKKESEFDGPVLSSICPGWVLYAEKTHPYVLPRMSTVKSPQQITGCLLKTLAAHELGVTRNDIYHLSIMPCFDKKLESARPEKYGEQNTSNDVDCVLTAKELVTLLEQHSDKFQLIPPQAHTITNSAIPVVDLYSKCAPRTWPLVQYSWSNDSGSASGGYGYNYLKMYQNHLIMKHPTKYQQEGFSIDYVKGRNTDLTEMRLMYGSEKLASSAIVNGFRNIQNLVRKLKPTVKPGSTTGKGNALVARRRARVAGGITKASSPAGSDESADASKCDYVEIMACPNGCINGR",
    "response#3": "VFVASISHQSRASLATAYNLSIEEIDKLLINLFINQMGFKYIVGTSIGRKLSLINEAQNLIEKKESEFDGPVLSSICPGWVLYAEKTHPYVLPRMSTVKSPQQITGCLLKTLAAHELGVTRNDIYHLSIMPCFDKKLESARPEKYGEQNTSNDVDCVLTAKELVTLLEQHSDKFQLIPPQAHTITNSAIPVVDLYSKCAPRTWPLVQYSWSNDSGSASGGYGYNYLKMYQNHLIMKHPTKYQQEGFSIDYVKGRNTDLTEMRLMYGSEKLASSAIVNGFRNIQNLVRKLKPTVKPGSTTGKGNALVARRRARVAGGITKASSPAGSDESADASKCDYVEIMACPNGCINGR"
  },
  {
    "reference": "LCADRVEEVQNVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLPFTNSYSFSKKENNSPEHLNEEVSMIQSLGFRNRTLRFRQSEPENPTLQETSLSAQLSNLGIVRSRRTKQQVQSQKKSVYIELGSDSSEDSANKGRYYSVRDHELLQITPQGARAEARLNPAKKAACEFSGDITNIEHHQSSNKDLPTTEKHAAEKHTEKYQGISVSNLHVEPCGTDTHASSLQHENSSLLLTKDRMNVEKAEFCNESKQPGLARSQQSRWAESKETCNDRQTPSTENRVILDADPLNGEKELPRSDHPRDSQDVPWITRSSSIQKVNEWFSRRDEILTSDGSHNGKSESNAEVAGAVEVANEVDGYSGSPGKITLLAHDPHGVLTCESERVHSKPVESNIEDKIFGRTYRRKASLPNLSHITENLIVGASAIEPQITQACPLTSKLKRKRRSTSGLHPEDFIKKVDLAVVQKTPVEIIKGTDRIEQNGHEMNITNNDHENETKGDCVQKEKNANLTESLEKESAFTSKAEPISSSISNLELELNVHSSKAPKKNRLKRKSSTRPIHALELVVNKNPSPSNHTELQIDSCSSSEEVKEKNSDQIPVRHSKKLQLMEGKEPATGAKKSNKSDEQINKKLASDAFLELNLTNIPGVVTNGLSSNKLQEFVDPNLQREETEENLGTIQMSNSTKDLKDLTLSGGRSVQTDRPKESTNIVLVPET",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. We need a protein that can perform S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +   [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-   cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. with high efficiency. The designed protein sequence is ",
    "response#1": "TIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPNHTIRKMIQGWCVEKGSA",
    "response#2": "TIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPNHTIRKMIQGWCVEKGSD",
    "response#3": "IPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPNHTIRKMIQGWCVER"
  },
  {
    "reference": "DSGSAIVALTNDRDTSYFGEIGIGTPPQKYTVIYDTGSSVLWVPSSKEQDFIEATDETDNVFLHRRFSFWLNRNVDEEEGGELVFGGLDPNHFRGDHTYVPVTYQYYWQFGIGDVLIGDKSTGFCAPGCQAFADSGTSLLSGPTAIVTQINHAIGANSEELNVKFGLTPEQYILKGEATQCISGFTAMDATLLGPLWILGDVFMRPYHTVFDYGNLLVGFAEAA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Aspartic protease with a high preference for bonds between hydrophobic residues.2. The designed protein must have at least one Peptidase A1 domain.3. A protein that contributes to proteolysis by functioning in extracellular region, endoplasmic reticulum, protein storage vacuole, membrane with aspartic-type endopeptidase activity.4. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "YLSPVTVGTSTVHLDFDTGSADLWVFSDLQAKSSLSGHDYYKTDASKRKSGYTWKISYGDGSGASGQVYTDKVTVGQVTATAQAVEAATSVSAQFSQDVDTDGLLGLAMSSINTVKPQQQTTWFDTVKSQLAKPLFAVTLKYHAAGTYDFGYIDSAKYTGAITYVNADASQGFWGFTASGYSVGTGATVSSSISGILDTGTTLMYVPAATAKAYYAKVSGAKLDSTQGGYVFPCSATLPNFSITVAGVKQTVPGKYINYAPITDGSSTCFGGMQPDTDIGQSIFGDIFLKSKYIVHDMSNGTPRLGFAR",
    "response#2": "YLGNFTVGTPPQPVSLVLDTGSANMWVIDASCDNMFCNGWIGSNYTRQKFDTSKSSSFSRENRKFSIQYGKGLCSGYLGTDTVGLGGGLTIRKQELGIANKLDVDFAVQPMDGIFGLAWPALAVDQITPPMQNLISQLDVPVFSVWLDRKIQASHGGSAGMITYGGIDTKNCDAGVTYVPLTAKTYWQFKMDGFAVGTYSQYGYNQVISDTGSSWISAPYAMINDIATQTHATWDEMNEIYTVKCSTMKTQPDLVFTIGGALFPVKSVEYILDIGLDEGKCALAISPLMASGFGPSWILGDVFIRQYCNIYDIGNARIGFS",
    "response#3": "EYLSPVTIGGQKFVMNLDTGSSDTWVFNTQLSEDAKRGHSIFDPAKSKAFSDLEDATFNITYGDASFAFGRVGIDTVDIGGATVQKQAVGLPTDVSGSFILDQASDGLIGLGFDELNTVEPQQQKSFFTNLAANLDEPVLAAQLKKGAPGSYEFGSIDETKFKGDLVTIPVNNSRGFWEVKSTMFKVGKDEQLHRITKGVGSAIADTGTTLMLVNEEIVNSYYDQVDNARSVYAAGGFIFPCNATLPDLYVSLGDTHLARIPGDLMNFSKVGLSTETGEELCFGGVQSNSGSGLQVFGDVLFKAIFVVFDLRGPSLHVQ"
  },
  {
    "reference": "MNEIDFAVLGTGGIGRRTLEVSQHKEQLRPVAACDRNGIALDRDGLDVEELLDATEGNIAGDGRGDTDGPDAATDGGVATDSGGVKQTGEGAGIVASEQGEPTATPIDDVIAVAEDLDAVLIALPNLEHDFIPRIADRFADAGYSGVLIDVLKRSRVIGMLDERAETFEEAGLTFVCGAGATPGFLTGAAALAAQSFVEVHEIDIWWGVGLKSGYKDNRGTVREDIAHLDGYDIETARDLSDDEIEEIVEAHDGVLEFHDMEHADDVLLERAGICDAEDVTVGGVLDVRSDEKPTTTTVTVTGTTFDGERATNTFELGDETSMAANVNGPALGYLQSAVRRNRAGEYGVFGPAELMPGF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that can modulate the overall flux of metabolites through the biotin biosynthesis, Cofactor biosynthesis pathway, enhancing or suppressing its activity as needed.2. The protein must exhibit the following characteristics: A 'suicide' enzyme that participates in biotin synthesis.3. The protein must exhibit NAD binding. The designed protein sequence is ",
    "response#1": "GKCPENCKWCAQSAHYKTQADVYDLVDKEECLRHAKYNESQGVARFSLVTSGRKPSDKQMEKLCEAVRHMRRNSSIQLCASLGLLNENEMQALHDAGVTRYHCNLETAPSYFPQLCSTHTQEEKLRTLQAARNVGMDICSGGIIGMGESIEQRIEFR",
    "response#2": "LCPEDCSYCSQRLGSKAGILKYTWLKPEEASKAAAAGVAGGAKRVCLVASGRGPTDRDVDRVTKTIEAIKEENEGIEVCACLGLLSDGQADRLRTAGADAYNHNLNTSEETYGAITTTHTYADRVETVQQAQAAGLSACSGLIAGMGESDKDLVDVF",
    "response#3": "LCPEDCSYCSQRLGSKAGILKYTWLKPEEASKAAAAGVAGGAKRVCLVASGRGPTDRDVDRVTKTIEAIKEENEGIEVCACLGLLSDGQADRLRTAGADAYNHNLNTSEETYGAITTTHTYADRVETVQQAQAAGLSACSGLIAGMGESDKDLVDVR"
  },
  {
    "reference": "MNFFLYFRTIFLIQLYFFNYSTFGCSASSTSVQSDTTNQVSVSCPKYTTIYTSGTSPDTKTIYPESTSTKSITTSTQSHSSPVIVVSTVGTVTETTISGSTEYTTTIPAEGITSGTVEIVEPTAGTVTETITSGTLPFTTTLAQASGTVSGTVEIVSPKNNPTTVYSGTVATTETFSSSTVVVIPTAICDGVRGLEYAVYDYTISSSMNEFCYPKNGQTDVFAFNEPAYFGSSDLDQSSPLFTGVFSSTDDIPEWASSWYLPPYPPQASDMASTYCACKVIVYQFFLRIPETDTYTLVVNNVDDVFFGWFGDKAISGWSNNNFDAYSYWHESPNMGLGTVGMGNFTVGNYPEGYFLPVRFVVANGAYIGGFDFYFTSDSTGPLATTSYSYTKTCTQQFLPFGQGNGGVNGPTEKLS",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in flocculation, potentially with improved properties such as stability, specificity or selectivity.2. The designed protein should contain one or more PA14 domains that are essential for its function. The designed protein sequence is ",
    "response#1": "INMAMGRVVVSPDLPVGSVIATKTWTMPDNNTIYVTCDRNTTLKSDAKVVAAGLVQGANKVYSTAIPGIGLRFSRKGAISMIYPDSYTTTGSSFRLVGSTFTLDIIKTSTTTGSGTLASGPYTEYGPGFTILKTSLNV",
    "response#2": "INMAMGRVVVSPDLPVGSVIATKTWTMPDNNTIYVTCDRNTTLKSDAKVVAAGLVQGANKVYSTAIPGIGLRFSRKGAISMIYPDSYTTTGSSFRLVGSTFTLDIIKTSTTTGSGTLASGPYTEYGPGFTILKTSLNF",
    "response#3": "ITGTNGKTTTTFLIDHILKQHHKKTGLIGTIENRINDKVIESSRTTPESYDLQKLLQNMKNEQVDNVIMEVSSHALSLDRVYGLSYKTAVFTNLSLDHLDYHKTMDNYLKAKAKLFKMSEYGVVNIDDKASNAILREAQCKKIYTYSTMNPKADFYANKIELNATGVVFNLIFDEKIYRVNYSTPGKFSVYNALAGIIL"
  },
  {
    "reference": "MTLDDLFTVRGSAANIANIRNLKAIQITIASPENIREWSYGEVKKPETINYRTFKPERDGLFCAKIFGPVKDYECNCGKYKRMKHRGIVCEKCGVEVIASKVRRERMGHIELAAPVAHIWFLKTLPSKIGTLLDMTMADLEKVLYFDSYIVLDPGSTNLTKMQVISEDQYLQVIDHYGEDALTVGMGAEAVRSLLEELNLEELRVQLREESQATKSQTKKKKLTKRLKIVEAFLESNNKPEWMVMEVIPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLMELGAPDIIIRNEKRMLQEAVDALFDNGRRGRAITGTNGRPLKSLSDMIKGKQGRFRQNLLGKRVDYSGRSVIVVGPKLKLHQCGLPKKMALELFKPFIYSELEKRGLASTIKSAKKMVEREELVVWDILEEVVREYPIMLNRAPTLHRLGIQSFEPLLVEGKAIQLHPLVCSAYNADFDGDQMAVHVPLSVEAQIECRVLMMSTNNILSPANGSPVIVPSQDIVLGLYYMTVDRSFEKGENMSFCAPWEVVAAYDAGVVALHARINVRMEDGKVVRTTVGRILVWELLPHCVPFSMVNTTLTKKNIARLVSTAYRDAGTKATVILCDRLKDVGYEYATRAGVTIAVKDLTIPSTKKGLIETAQNEVDDIERQYRDGIITRTEKYNKVVDVWTKATQDVSNEMIREISSDIVEDPRTGAKEANSSFNSIYMMSTSGARGNQDQMRQLAGMRGLMAKPSGEIIETPITSSFREGLSVLQYF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Zn(2+), Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.2. The designed protein should have DNA binding, DNA-directed 5'-3' RNA polymerase activity, magnesium ion binding, zinc ion binding within DNA-directed RNA polymerase complex to regulate DNA-templated transcription.3. The presence of Zn(2+) is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "EWMILDVIPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKKLMELGAPDIIIRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKIYQCGLPKEMALELFKPFVMKKLVEKGICNNIKNAKKAVERQRSEVWDILEEVIKDHPVLLNRAPTLHRLGIQAFEPVLVEGRAIRLHPLVCTAYNADFDGDQMAVHVPLSAEAQAEARFLMLSANNLLKPADGKPIVVPTQDMVLGIYYLL",
    "response#2": "GLTITDYLMSGYGARKGIVDTALKTANSGYLTRRLIDIAQDIVIREKDCLTSSSFLIDSQTKFDTEQLIGRVLLKSVYDSKTEKLIAKANTQLTLDLLNLFNQKQISKFYIRSPLTCNLYHSICQMCYGWDLSNQNLVDLGEAVGILAGQSIGEPGTQLTMRTFHTGGIFTSEARQQIISPKNGIIKFSKILKTIILRTNRGEDVLLTKNSGSLILIPEKHTDEIIQIELLRNTMLFVKSNQYIKQSAIIGELISTDKQTLTERKPILSDTAGEIFIPRLKTRMNLITQNRLLWILSGQVYQAPSNSFLNFYTDHKINKNSYIFRTKLINQYSGYTKVLNQNKNLLEQKIQITNETYFLL",
    "response#3": "GLTITDYLMSGYGARKGIVDTALKTANSGYLTRRLIDIAQDIVIREKDCLTSSSFLIDSQTKFDTEQLIGRVLLKSVYDSKTEKLIAKANTQLTLDLLNLFNQKQISKFYIRSPLTCNLYHSICQMCYGWDLSNQNLVDLGEAVGILAGQSIGEPGTQLTMRTFHTGGIFTSEARQQIISPKNGIIKFSKILKTIILRTNRGEDVLLTKNSGSLILIPEKHTDEIIQIELLRNTMLFVKSNQYIKQSAIIGELISTDKQTLTERKPILSDTAGEIFIPRLKTRMNLITQNRLLWILSGQVYQAPSNSFLNFYTDHKINKNSYIFRTKLINQYSGYTKVLNQNKNLLEQKIQITNETYFLGS"
  },
  {
    "reference": "MKRDDTYRRVTREKVVVRGNTTIRTLDQRTSFLPLLDKVTREKVVVRGNTTIRTLDQRTSFLPLLDNPAPSTSGFSQMARMMDSLILDNLAPIAFTKITTRHHPAHNVKKASSSQSIPQTNNVALGGINRSGGGIQKAKIPTQEVPPNPRSQPISVVPPQNATNSPPTASDSRWISSLRRRDPEIQPTLPSISSSYTHHTSHGYTSNADKRSIRSTIKSSMRKSRSSDRLRQRKLATSKKSSRKKSSSEEAGASDDQEATSIEDNTPPLRRSPRKDKMKVFNSTGYESHLVDTLEKDILQRHPNVYWHDVAGLNEAKAILQEAVVLPVIMPDFFKGIRRPWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPSTIFIDEIDSLCCARGSDSEHEASRRFKAELLIQMDGLSGMHDEKIIMVLAATNHPWDIDEAFRRRFEKRVFIGLPDDETRAALLKLCLKGVNVASDLDLKSVAEKLKGFTGSDITNVCRDASMMNMRRRIVGKSPAEIKLIKREEVDLPVTAQDFDEAMNRTRKNSKLDTSVNL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation.2. The designed protein should have a binding site that is specific to the target ATP ligand.3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.4. The ideal protein would be one that plays a critical role in cell cycle, cell division through microtubule binding within spindle pole, microtubule, centrosome. The designed protein sequence is ",
    "response#1": "LPRSILLFGPRGTGKTLLGRCIASQLGATFFKIAGSGLVAKWIGEAEKIIHASFLVARCRQPSVIFVSDIDMLLSSQVSEEHSPVSRMRTEFLMQLDTVLTSAEDQIVVICATSKPEEIDESLRRYFMKRLLIPLPGDVDLVKIAQKMEGYSGADITNVCRDASLMAMRRRIEGLTPEELDMQAVADKLEGYTGSDISNVCRDAALMSMRRKVEGMTPEEVRPVTYQDFENAFCKIQPSISQKELDMYVEWNKMFGS",
    "response#2": "VLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPSTIFIDEIDSLCACRGSDSEHEASRRFKAELLIQMDGLNATNDEKIIMVLAATNHPWDIDEAFRRRFEKRVYIF",
    "response#3": "VLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARSYSPSTIFIDEIDSLCSQRGSDSEHEASRRFKAELLIQMDGLNSETNEDGKTIMVLAATNHPWDIDEAFRRRFEKRIYIPC"
  },
  {
    "reference": "MAKDKKTNPESKKSFPTAFFFLLFGVIFGVVTVQNFFSAKKASVGFSHQLEHLVNLKLLIPEESRKTALNDNLVSFSGRFREVVPAEGQVRYQYLDLIERKHQIDFELEEASKSLTVLSKEVRNAITWFSAISGMPIPEAGYTISPRTDVGLSVLEPLVVYGPVDAQIVNLAALENRVRSLPKSTESLRVFGSDLYALIGKYLSPALGIGSESLKKEIKDLHQQVENSLTQVIEGDQAVALYKTVLETLHRISLALVSPEEGTRFHQLRSVRLYREDFNRCVKLLRESDETQVQLDKLRGELVQAVWYFNNQELSSRALEKQDPEVFSRWFEGAKQEWAAFSSNKSLSFRAPDQPRNLVLEKTFRSEEPTPHYSGYLFTFMPIILVLLFIYFIFSRQVKGMNGSAMSFGKSPARLLAKGQNKVTFADVAGIEEAKEELVEIVDFLKNPTKFTSLGGRIPKGILLIGAPGTGKTLIAKAVAGEADRPFFSIAGSDFVEMFVGVGASRIRDMFEQAKRNAPCIIFIDEIDAVGRHRGAGIGGGHDEREQTLNQLLVEMDGFGTNEGVILMAATNRPDVLDKALLRPGRFDRRVVVNLPDIKGRFEILAVHAKRIKLDPTVDLMAVARSTPGASGADLENLLNEAALLAARKDRTAVTAVEVAEARDKVLYGKERRSLEMDAQEKKTTAYHESGHAIVGLCVEHSDPVDKVTIIPRGLSLGATHFLPEKNKLSYWKKELYDQLAVLMGGRAAEQIFLGDVSSGAQQDIAQA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit ATP binding, metalloendopeptidase activity.2. The Cytoplasmic topological domain of the protein should be located on the surface of the protein to facilitate interactions with other molecules or cells. The designed protein sequence is ",
    "response#1": "IPKGVLLYGPPGTGKTLLARAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFEQAKQNAPCIIFVDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFDSRGGIILIAATNRPDILDPALLRPGRFDRQIPVAAPTFEDI",
    "response#2": "VLMVGPPGTGKTLLARAIAGEAKVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFEGNEGVIVIAATNRPDVLDPALLRPGRFDRQVVVPY",
    "response#3": "IPKGVLLYGPPGTGKTLLARAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFEQAKQNAPCIIFVDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFDSRGGIILIAATNRPDILDPALLRPGRFDRQIPVAAPTLE"
  },
  {
    "reference": "MCAMDSESLGFYTGRCILDFPPKSSRSSTWYVAAAGFCAFSSSLLAGSYSTSQSEEDSEENAHDQQNVKRKARVVFVLGGPGSGKGTQCEKIVENFGFTHLSAGDLLRAEINSGSQYGVMIQNTIKEGKIVPSEVTVHLLQKAMNGSDNDKFLIDGFPRNEENRAAFEAVTGIEPEFVIFFDCPQEELEKRLINRNQGRVDDNIDTIRKRFKVFEESTLPVVQYYERKQKLQKINGAREKEEVFKSLKPLFLPFLEEELLQLTKELLNGIDEGDYALYKRLCDPSLTAFEPDAQGHLVEGLPFHKFYLDMSKRFPSPSKPHSQVSSPKITFIGDEVALVTYTRLVQSTKDGVPCVEAFNETRIWQRGLDDSGIAVWKLIHFHKSKCPLHRAHHKSKGSPHSCKE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein have Calcium/calmodulin-dependent protein kinase II association-domain domains with specific sequence motifs that are crucial for its activity.2. A protein that contributes to 'de novo' pyrimidine nucleobase biosynthetic process by functioning in cytoplasm, nucleus with ATP binding, calmodulin binding, calmodulin-dependent protein kinase activity, uridylate kinase activity.3. For general function, the protein need meet that Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP; Has preference for UMP and CMP as phosphate acceptors.; Plays an important role in de novo pyrimidine nucleotide biosynthesis4. We need a protein that can perform ATP + dCMP = ADP + dCDP with high efficiency.5. Mg(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "MNEREVMAATRAQIEAATDNDTAAYAAMCCDDATGAGCTGSAAIALKTPADVETWGSKGASANAAGTPFELLNPRMQIMGNVALLSYLRQNGGGDAGREAAVEETRVWLGKGGRWRLKHLHCSTSRKSSGAPEREVMAATRAQIEAATDNDTAAYAAMCCDDATGAGCTGSAAIALKTPADVETWGSKGASANAAGTPFELLNPRMQIMGNVALLSYLRQNGGGDAGREAAVEETRVWLGKGGRWRLKHLHCSTG",
    "response#2": "MEREVMAATRAQIEAATDNDTAAYAAMCCDDATGAGCTGSAAIALKTPADVETWGSKGASANAAGTPFELLNPRMQIMGNVALLSYLRQNGGGDAGREAAVEETRVWLGKGGRWRLKHLHCSKK",
    "response#3": "MREREVMAATRAQIEAATDNDTAAYAAMCCDDATGAGCTGSAAIALKTPADVETWGSKGASANAAGTPFELLNPRMQIMGNVALLSYLRQNGGGDAGREAAVEETRVWLGKGGRWRLKHLHCSSSEREVMAATRAQIEAATDNDTAAYAAMCCDDATGAGCTGSAAIALKTPADVETWGSKGASANAAGTPFELLNPRMQIMGNVALLSYLRQNGGGDAGREAAVEETRVWLGKGGRWRLKHLHCSSS"
  },
  {
    "reference": "MSNKPDANDQCMVKETISRMSMSKEKPFLRRNYSSPSFNDSVNSVNDEFQTYQLPNGEFSDNMNDLYFPFRKGNESLMTENSNYPISEQDTQHNHISDFPSRRMQLDGMNHSNNDTYSTAIPFSKDSSVFDAVGQQSSVPIHINPAYTNSHQNSYSLNETYLSYDFFDNHRGASSSAVSKDGLASPRPTKDVDADDTTWYFTSSPSFQPLKDSDRSQTKNTHEETSPIVSSPEDDADAAFKPSSLPSTSISASSNAFYKPAAPDNERPLDPHITPYLRLQLLTTGADDNDIRKNALTQVDYLSYDWKETDIWASWREITKTKANYDNGIRLENASWRTWMKHKFKLKTISPETLNWLKECDVTWLYGPLLHTSMPSRHHSHRKKEKEKEKSKAPRVTPLTHKTNDDEANHSETSSGSSLAKKPILKRRTPQELLLSGRDLTPWPQIRRFDSLLARNRGDIFSNRNHATIRSALFSQRYPSHSKRHIHFNDRVQQCIAVDIDSLPSDSESASYNTDDANSVVSPSKDGISTTTVSSDATRSSQSSHFRIIEDLPDSKLKYSLEDQATYDQSSRYPGRHFGKTGRSHFPRAPEYYGENDFEEDEVYRDDRHYENEHDEYDPNLIYYDNEPTEENRLVFEDTNNTFIDTDSDDSNADESQFLEYANDSPNSSESLESLNNQSYSSSPYSVFSHPPPYMGRQSLNDSPQTSDFKASNLNDSSSNVHSIFQTRETTSPSVQNKTPTKYHRELKSSKDGHEQASPLVSSSPSGS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have protein phosphatase regulator activity within cytoplasm, fungal-type vacuole membrane, cytosol to regulate signaling, regulation of carbohydrate metabolic process, protein catabolic process in the vacuole. The designed protein sequence is ",
    "response#1": "WGLARISHKSVKYDDIGKYVYDSSAGDNITAYVVDTGVSIHHVEFEGRASWGATIPSGDVDEDNNGHGTHVAGTIASRAYGVAKKAEIVAVKVLRSSGSGTMADVIAGVEWTVRHHKSSGKKTSVGNMSLGGGNSFVLDMAVDSAVTNGVIYAVAAGNEYDDACYSSPAASKKAITVGASTINDQMAYFSNYGSCVDIFAPGLNILSTWIGSNTSTNTISGTSMATPHVAGLSAYYLGLHPAASASEVKDAIIKMGQ",
    "response#2": "WGLARISHKSVKYDDIGKYVYDSSAGDNITAYVVDTGVSIHHVEFEGRASWGATIPSGDVDEDNNGHGTHVAGTIASRAYGVAKKAEIVAVKVLRSSGSGTMADVIAGVEWTVRHHKSSGKKTSVGNMSLGGGNSFVLDMAVDSAVTNGVIYAVAAGNEYDDACYSSPAASKKAITVGASTINDQMAYFSNYGSCVDIFAPGLNILSTWIGSNTSTNTISGTSMATPHVAGLSAYYLGLHPAASASEVKDAIIKMGI",
    "response#3": "NITAYVVDTGVSIHHVEFEGRASWGATIPSGDVDEDNNGHGTHVAGTIASRAYGVAKKAEIVAVKVLRSSGSGTMADVIAGVEWTVRHHKSSGKKTSVGNMSLGGGNSFVLDMAVDSAVTNGVIYAVAAGNEYDDACYSSPAASKKAITVGASTINDQMAYFSNYGSCVDIFAPGLNILSTWIGSNTSTNTISGTSMATPHVAGLSAYYLGLHPAASASEVKDAIL"
  },
  {
    "reference": "MLEKTTLKNIILIHFLMFLAVVTAQTAPDEDTSAITRCTAEGTNPVRETDIPNPVNVGTIDDRSCYANYKESTVYGKTWGVYNITFDSNDFDTSLQPRIERSLSRSSETGIGSYARLTGVFRILEVGDTSGTSQDGTYLAQAKGKHTGGGGSPDPAICLYLAKPVYGTGEDADKQVSFDIYAERILYRGGEGDGREIVFLKNVKKDEETNFELEVGFKEDPNDVSKKIQYCNAVIGGDTFNWNIPEPERGTESGIRYGAYRVKGGRAQIRWANTTYQKVENVEVTNPGPIGDVYKLKNVATGQYLSDSGVSASAVIMSDSGEAQNNYWTFVESGSLFNIDNETFGILRAPGAGGPGGAYVVVSTTKEGPSSDGDKVWTIHYNESNDTYRFESGSSGRFMYQEINGNVTHISAMNTDDRSVWKAIAVESLSVDENAILASDVRVFPNPASDSFTISLKTINHVTVNIYDVLGNTIFKSEFNGDTIQIRNKGQFKAGVYLIQLTDKNNNKYHKKLIVK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The Ca(2+) binding site should be stable and able to withstand changes in environmental conditions.2. The Proton donor/acceptor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "RIDFKEGKLSPEWIHLQNPEAKNYIFTKDGKLRLIATPVTLSDWKSPTFVALRQEHFDMEASAPVVLQKAGVNDEAGISVFMEFHSHYDLFVRQDKDRKRSVGLRYKLGEITHYAKEVSLPTDGEVELVVKSDINYYYFGYKVNGIYHDLGKMNTRYLSTETAGGFTGVVLGLYITSASKDSKAYADFEYFKYI",
    "response#2": "RIDFKEGKLSPEWIHLQNPEAKNYIFTKDGKLRLIATPVTLSDWKSPTFVALRQEHFDMEASAPVVLQKAGVNDEAGISVFMEFHSHYDLFVRQDKDRKRSVGLRYKLGEITHYAKEVSLPTDGEVELVVKSDINYYYFGYKVNGIYHDLGKMNTRYLSTETAGGFTGVVLGLYITSASKDSKAYADFEYFKYL",
    "response#3": "RIDFKEGKLSPEWIHLQNPEAKNYIFTKDGKLRLIATPVTLSDWKSPTFVALRQEHFDMEASAPVVLQKAGVNDEAGISVFMEFHSHYDLFVRQDKDRKRSVGLRYKLGEITHYAKEVSLPTDGEVELVVKSDINYYYFGYKVNGIYHDLGKMNTRYLSTETAGGFTGVVLGLYITSASKDSKAYADFEYFKYN"
  },
  {
    "reference": "MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have a high affinity for pyridoxal 5'-phosphate.2. The desired protein should be able to perform 2-oxoglutarate + cadaverine = 5-aminopentanal + L-glutamate3. We require a protein that can specifically bind to the metabolites produced in the Amino-acid degradation pathway.4. The protein design should prioritize the formation of helix-helix-helix-helix-strand-strand-strand-turn-helix-strand-helix-strand-helix-turn-strand elements.5. The designed protein should be involved in putrescine catabolic process, L-lysine catabolic process by localizing to cytosol and exhibiting pyridoxal phosphate binding, butane-1,4-diamine:2-oxoglutarate aminotransferase activity, diamine transaminase activity, identical protein binding.6. The protein design should incorporate a binding pocket that specifically recognizes pyridoxal 5'-phosphate to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "DIINIGVGDPDKPTPAHILQAMREAIDEASNHNYPPYEGTQEFREAAVKWMERRFGVVDLNPNTEVVSSIGSKEAIHNTFLAFVEAGDYTLIPDPGYPVYRTSTIFAGGEPFTMPLKAENKFLPDLDLIPEEVARKAKMLWVNYPNNPTGALATLEFFEELVAFCQQHSILLCHDHAYSEMAYDGYKPPSVLQIPGAKDIAIEFHSLSKSYNMTGWRIGFAVGNAYAIQGLSQVKTNVDSGVFKAIQKAAIAAYNTDEVELQAVMSVYQNRRDIIVKGLQSLGWPIEPPKATLYVWVPVPPGYTSTEFTTLLLDKCGIVVPPGVGYGVSGEGYFRIALTICEERLHEAIQAAL",
    "response#2": "DIINIGVGDPDKPTPAHILQAMREAIDEASNHNYPPYEGTQEFREAAVKWMERRFGVVDLNPNTEVVSSIGSKEAIHNTFLAFVEAGDYTLIPDPGYPVYRTSTIFAGGEPFTMPLKAENKFLPDLDLIPEEVARKAKMLWVNYPNNPTGALATLEFFEELVAFCQQHSILLCHDHAYSEMAYDGYKPPSVLQIPGAKDIAIEFHSLSKSYNMTGWRIGFAVGNAYAIQGLSQVKTNVDSGVFKAIQKAAIAAYNTDEVELQAVMSVYQNRRDIIVKGLQSLGWPIEPPKATLYVWVPVPPGYTSTEFTTLLLDKCGIVVPPGVGYGVSGEGYFRIALTICEERLHEAIQRMQQ",
    "response#3": "DIINIGVGDPDKPTPAHILQAMREAIDEASNHNYPPYEGTQEFREAAVKWMERRFGVVDLNPNTEVVSSIGSKEAIHNTFLAFVEAGDYTLIPDPGYPVYRTSTIFAGGEPFTMPLKAENKFLPDLDLIPEEVARKAKMLWVNYPNNPTGALATLEFFEELVAFCQQHSILLCHDHAYSEMAYDGYKPPSVLQIPGAKDIAIEFHSLSKSYNMTGWRIGFAVGNAYAIQGLSQVKTNVDSGVFKAIQKAAIAAYNTDEVELQAVMSVYQNRRDIIVKGLQSLGWPIEPPKATLYVWVPVPPGYTSTEFTTLLLDKCGIVVPPGVGYGVSGEGYFRIALTICEERLHEAIQAL"
  },
  {
    "reference": "MTVDALTQPHHLLSLAWNDTQQHGSWFAPLVTTSAGLLCLLLYLCSSGRRSDLPVFNPKTWWELTTMRAKRDFDANAPSWIESWFSQNDKPIRFIVDSGYCTILPSSMADEFRKMKELCMYKFLGTDFHSHLPGFDGFKEVTRDAHLITKVVMNQFQTQAPKYVKPLANEASGIITDIFGDSNEWHTVPVYNQCLDLVTRTVTFIMVGSKLAHNEEWLDIAKHHAVTMAIQARQLRLWPVILRPLVHWLEPQGAKLRAQVRRARQLLDPIIQERRAERDACRAKGIEPPRYVDSIQWFEDTAKGKWYDAAGAQLAMDFAGIYGTSDLLIGGLVDIVRHPHLLEPLRDEIRTVIGQGGWTPASLYKLKLLDSCLKESQRVKPVECATMRSYALQDVTFSNGTFIPKGELVAVAADRMSNPEVWPEPAKYDPYRYMRLREDPAKAFSAQLENTNGDHIGFGWHPRACPGRFFASKEIKMMLAYLLIRYDWKVVPDEPLQYYRHSFSVRIHPTTKLMMRRRDEDIRLPGSL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that The 2- methylbutyrate moiety of lovastatin is synthesized by the lovastatin diketide synthase lovF via condensation of one acetyl-CoA unit and one malonyl-CoA unit; The first step in the biosynthesis of lovastatin is the production of dihydromonacolin L acid by the lovastatin nonaketide synthase lovB and the trans-acting enoyl reductase lovC via condensation of one acetyl-CoA unit and 8 malonyl-CoA units; Next, dihydromonacolin L acid is oxidized by the dihydromonacolin L monooxygenase lovA twice to form monacolin J acid2. The protein have enhanced monacolin L carboxylate + O2 + reduced [NADPH--hemoprotein   reductase] = H(+) + H2O + monacolin J carboxylate + oxidized [NADPH--   hemoprotein reductase].3. The protein must have high specificity towards monooxygenase activity, heme binding. The designed protein sequence is ",
    "response#1": "IAMVGMGCRFGGGATDPQKLWKLLEEGGSAWSKIPPSRFNVGGVYHPNGQRVGSMHVRGGHFLDEDPALFDASFFNMSTEVASCMDPQYRLILEVVYEALEAAGIPLEQVSGSKTGVFAGTMYHDYQGSFQRQPEALPRYFITGNAGTMLANRVSHFYDLRGPSVSIDTACSTTLTALHLAIQSLRAGESDMAIVAGANLLLNPDVFTTMSNLGFLSSDGISYSFDSRADGYGRGEGVAAIVLKTLPDAVRDGDPIRLIVRETAINQDGRTPAISTPSGEAQECLIQDCYQKAQLDPKQTSYVEAHGTGTRAGDPLELAVISAAFPGQQIQVGSVKANIGHTEAVSGLASLIKVALAVEKGVIPPNARFLQPSKKLLKDTHIQIPLCSQSWIPTDGVRRASINNFGFGGANAHAIVEQYGSHETPRVLTHLPPPEEEHPHMNRVNIKAFYHELGLMGYNYSKDFRRLHNMQRADLRASGTIDFIPLMDEGNGCPLLLHPASLDVAFQTVIGAYSSPGDRRLRCLYVPTHVDRITLVPSLCLATAESGCEKVAFNTINTYDKGDYLSGDIAVFDAEQTTLFQVENITFKPFSCVIRETGVNQDGRTTGITMPNHSAQEALIKATYAQAGLDITKAEDRCQFFEAHGTGTPAGDPQEAEAIATAFFGHEQVARSDGNERAPLFVGSAKTVVGHTEGTAGLAGLMKASFAVRHGVIPPNLI",
    "response#2": "IAMVGMGCRFGGGATDPQKLWKLLEEGGSAWSKIPPSRFNVGGVYHPNGQRVGSMHVRGGHFLDEDPALFDASFFNMSTEVASCMDPQYRLILEVVYEALEAAGIPLEQVSGSKTGVFAGTMYHDYQGSFQRQPEALPRYFITGNAGTMLANRVSHFYDLRGPSVSIDTACSTTLTALHLAIQSLRAGESDMAIVAGANLLLNPDVFTTMSNLGFLSSDGISYSFDSRADGYGRGEGVAAIVLKTLPDAVRDGDPIRLIVRETAINQDGRTPAISTPSGEAQECLIQDCYQKAQLDPKQTSYVEAHGTGTRAGDPLELAVISAAFPGQQIQVGSVKANIGHTEAVSGLASLIKVALAVEKGVIPPNARFLQPSKKLLKDTHIQIPLCSQSWIPTDGVRRASINNFGFGGANAHAIVEQYGHLPPPEEEHPHMNRVNIKAFYHELGLMGYNYSKDFRRLHNMQRADLRASGTIDFIPLMDEGNGCPLLLHPASLDVAFQTVIGAYSSPGDRRLRCLYVPTHVDRITLVPSLCLATAESGCEKVAFNTINTYDKGDYLSGDIAVFDAEQTTLFQVENITFKPFSG",
    "response#3": "IVVVGSGCRFPGDANTPSKLWELLQHPRDVQSRIPKERFDVDTFYHPDGKHHGRTNAPYAYVLQDDLGAFDAAFFNIQAGEAESMDPQHRLLLETVYEAVTNAGMRIQDLQGTSTAVYVGVMTHDYETVSTRDLESIPTYSATGVAVSVASNRISYFFDWHGPSMTIDTACSSSLVAVHLAVQQLRTGQSSMAIAAGANLILGPMTFVLESKLSMLSPSGRSRMWDAGADGYARGEAVCSVVLKTLSQALRDGDTIECVIRETGVNQDGRTTGITMPNHSAQEALIKATYAQAGLDITKAEDRCQFFEAHGTGTPAGDPQEAEAIATAFFGHEQVARSDGNERAPLFVGSAKTVVGHTEGTAGLAGLMKASFAVRHGVIPPNLLFDKISPRVAPFYKNLRIPTEATQWPALPPGQPRRASVNSFGFGGTNAHAIIEEYMDHDEVTVWNAAPCPILPRDQVYVRVEAVAINPSDTKMRGQFATPWAFLGTDYAGTVVAVGSDVTHIQVGDRVYGAQNEMCPRTPDQGAFSQYTVTRGRVWAKIPKGLSFEQAAALPAGISTAGLAMKLLGLPLPSPSADQPPAHSKPVYVLVYGGSTATATVTMQMLRLSGYIPIATCSPHNFDLAKSRGAEEVFDYRAPNLAQTIRTYTKNNLRYSLDCITNVESTTFCFAAIGRAGGHYVSLNPFPEHAATRKMVTTDWTLGPTIFGEGSTWPAPYGRPGSEEERQFGEDLWRIAGQLVEDGRLAHHPLRVVQGGFDQIKQGMELVRKGELSGEKLV"
  },
  {
    "reference": "MRVRGTQMNCPNLWKWGILIIGLVIICSASDKLWVTVYYGVPVWKDADTTLFCASDAKAHEREVHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTEAHLNLTKVNNSSNIDKPSITPNMTGNITDEVKNCTFNMTTEIRDKNQKVHALFYKLDIVEIAENSSEYRLINCNTSVIKQACPKVTFDPIPIHYCTPAGYAILKCNDKNFTGKGECKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENFTNNAKTIIVQLNKSIEINCTRPFNNTRTSMSIGPGQVFYRTGDIIGDIRKAYCEVNRTKWNTALKQVAEKLKKHFNNKTIIFQQQPPSGGDLEITMHHFNCKGEFFYCNTSQLFNNTCINNETMQGCHYNGTIILPCKIKQIINMWQGVGQAMYAPPIRGRINCLSNITGILLIRDGGNTTNNETFKPGGGNMKDNWRSELYKYKVVQIEPLGIAPTGAKRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAXEAQQHLLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKIICTTNVPWNSTWSNKSYKDIWDKMTWTQWEREISNYTDQIYEILTESQNQQDRNEKDLLELDKWASLWNWFDITNWLWYIRIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTPIHQQREPDRPERIEEGGGEQDRDRSVRLVSGFLALAWDDLRSLC",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Transmembrane protein gp41: Acts as a class I viral fusion protein; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes2. The protein design should include a Di-leucine internalization motif, YXXL motif; contains endocytosis signal motif to ensure proper function.3. The protein should contain a Helical membrane-spanning region.4. The ideal protein would be one that plays a critical role in fusion of virus membrane with host endosome membrane, clathrin-dependent endocytosis of virus by host cell, actin filament reorganization through structural molecule activity within host cell endosome membrane, plasma membrane. The designed protein sequence is ",
    "response#1": "MRVMGIQRNCQHWWIWGILGFWMLMICNVVGKLWVTVYYGVPVWRDADTTLFCASDAKAYDKEVHNVWATHACVPTDPNPQEISLENVTEEFNMWKNNMVEQMQTDIISLWDQSLKPCVKLTPLCVTLNCNNPNSSSTSFNLWVTVYYGVPVWKDAKTTLFCASDAKAYETEVHNVWATHACVPTDPNPQEMVLKNVTENFNMWKNDMVDQMHEDVISLWDESLKPCVKLTPLCVTLNCSNYNSNGTHNVSQNEPYNESEREIKNCTFNTTTEVNDKKEKVRALFYRLDIVPLNEKNSSNSSTSESYRLINCNTSAMTQACPKVSFDPIPIHYCTPAGYAILKCNDKKFNGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIISSENLTNNAKTIIVHLNESVEIVCTRPNNNTRKSIRIGPGQTFYATGDIIGDIRQAHCNISRSNWTKTLQKVKGKLEKHFPNKTLIFNSSSGGDLEITTHSFNCRGEFFYCNTSGLFNDTMLNDTESNSSIITIPCRIKQIINMWQEVGRAMYAPPIAGNITCKSNITGILLVHDGGEPNKTEDTFRPGGGDMRDNWRSELYKYKVVEIKPLGVAPTEAKRRVVQREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGMWGCSGKLICTTAVPWNRTWSNKTQDDIWNNMTWMQWEREINNYTDTIYSLLEESQNQQEKNEKDLLALDSWNNLWSWFSITNWLWYIRIFIMIVGGLIGLRIIFAVISIVNRVRQGYSPLSFQTLT",
    "response#2": "MRVKGTKKNYQHLWRWGTMLLGMLMICSATEQKWVTVYYGVPVWKEAVTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDANLNCTNANLNCTNATSTSSNVTNNATSRGWGKLEAGEVQNCSFDVTTYRKDKMRKEYALFYKVDVVPISEDNSANNNSTNNTYKTYRLISCNTSVITQACPKVTFEPIPIHYCAPAGFAILKCNDKNFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSANFSDNAKNILVQLNESVEINCTRPHNNTRRSITIGPGRAFYATGAIIGDIRKAYCELDATKWKNTLKKVVGKLREQYKNKTITFQPHSGGDPEVVMHIFNCRGEFFYCNLTNLFDSTWNGTESNDTIGNGTVILPCRIKQIINMWQEVGKAMYAPPIAGLINCLSNITGLILTRDGGNTTNTTDTETFRPGGGDMRDNWRNELYKYKVVKVEPLGVAPTRAKRRTVQREKRAVGIGALFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLRDQQLLGIWGCSGKLVCTTAVPWNSSWSNKSKTEIWDNMTWMQWDKEISNYTNTIYKLLEDSQIQQENNEKDLLALDKWNNLWSWFNITNWLWYIRIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLLFQT",
    "response#3": "MRVKGIRRNYQHLWRWGIMLLGMLMICSATEKKWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLENVTESFNMWKNNMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTHNVTATNNSTGWGKEEIKNCSFNITTELRDKRQKVYSLFYRLDVVPIEEGQGNSSNSGYKEYRLINCNTSAITQACPKVTFNPIPIHYCTPAGYAILKCNEETFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEKEVIIRSENITNNVKNIIVQLAEPVRINCTRPNNNTRTSIRIGPGQTFYTSNIIGDIRQAHCNVSRAEWNKALNKIGKQLRKYFVNKTIKFANSSGGDLEITTHSFNCEGEFFYCNTSGLFNGTWNASMQESNSTESNETIILPCRIKQIINMWQRVGQAMYAPPIQGIIKCVSNITGLILTRDGKSSNSTDEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRAVGREKRAALGALFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLKAIEAQQHLLQLTVWGIKQLQARVLALERYQRDQQLLGLWGCSGKIICTTNVPWNSTWSNKSYEEIWNNMTWIEWEREISNYTGQIYAILTEAQDQQDRNEKDLLALDQWTSLWNWFSITHWLWYIKIFIMIVGGLIGLRIVFAVLSIVNRVRQGYSPLSLQTRFPAPRGPDRPE"
  },
  {
    "reference": "MPQRKISFYRLLPLATLLLAACSTTPPSGPATSPTSPQWRQHEQQLRQLSQFQTRGAFAYLSEKQKVYARFFWQQTAPERYRLLLTNPLGSTELELVVQPGVTQLTDNQGKRHVSNDPQEMIQKLTGMSIPLESLRQWILGLPGDTTDFTLDDKYRLKQLTYQQDGVTWVVDYQEYNTQVTPALPSRMELSQGGQRIKLKMDNWTVK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the LolA protein..2. The protein should be optimized for stability and expression in cell outer membrane where it will participate in lipoprotein localization to outer membrane. The designed protein sequence is ",
    "response#1": "MTS",
    "response#2": "SCFVC",
    "response#3": "MVPL"
  },
  {
    "reference": "MEPVDPSLEPWKHPGSQPRTACTPCYCKQCCFHCQVCFITKGLGISYGRKKRRQRRRPPQGSQTHQVSLSKQPTAPARGDPTGPEESKEKVETETETHPGH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that contributes to suppression by virus of host translation initiation, suppression by virus of host type I interferon-mediated signaling pathway by functioning in host cell nucleolus, host cell cytoplasm with protein serine/threonine phosphatase inhibitor activity, cyclin binding.2. For general function, the protein need meet that Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to promote processive transcription elongation by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts; The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive; This also contributes to the increase in proviral transcription rate, especially when the provirus integrates in transcriptionally silent region of the host genome; Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication; Through its interaction with host TBP, Tat may also modulate transcription initiation; Besides its effect on RNA Pol II processivity, Tat induces chromatin remodeling of proviral genes by recruiting the histone acetyltransferases (HATs) CREBBP, EP300 and PCAF to the chromatin3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.4. The designed protein should have a binding site that is specific to the target Zn(2+) ligand. The designed protein sequence is ",
    "response#1": "MEPVDPNLEPWNHPGSQPTTACSKCYCKKCCYHCPVCFLNKGLGISYGRKKRKQRRGTPQGSKDHQNPVPKQPLPTTRGNPTGPEESKKKVASKTETDPCA",
    "response#2": "MEPVDPNLEPWNHPGSQPKTPCNKCYCKKCSYHCLVCFLTKGLGISYGRKKRRQRRRAPQGNRDHQNPIPKQPSSQPRGDPTGPKESKKKVESKAETDQFD",
    "response#3": "MEPVDPNLEPWNHPGSQPTTPCSKCYCKKCCWHCQLCFLKKGLGISHGRKKRKHRRGTPQSSKDHQNPIPKQPLPTTRGNPTGPKESKKEVESKAETDRFD"
  },
  {
    "reference": "MAERADLVFHNKAIDGTAMKRLISRLIDHFGMAYTSHILDQVKTLGFQQATTTSISLGIDDLLTIPSKGWLVQDAEQQSLILEKHHNYGNVHAVEKLRQSIEIWYATSEYLRQEMNPNFRMTDPSNPVHIMSFSGARGNASQVHQLVGMRGLMSDPQGQMIDLPIQSNLREGLSLTEYIISCYGARKGVVDTAVRTSDAGYLTRRLVEVVQHIVVRRTDCGTTRGISVSPRNGMITERIFIQTLIGRVLADDLYMGSRCIAIRNQDIGVGLVNRFITFRSQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVELGEAVGIIAGQSIGEPGTQLTLRTFHTGGVFTGGTAEHVRAPDNGKIKFNEGLVYPTRTRHGYPAFLCYIDLSVTIEGPDILHNVNIPPKSFILVKNDQYVESEQVIAEIRAEASTLNFKERVRKHIYSDSEGEMHWSTAVYHAPEYTYGNVHLLPKTSHLWILSGVPQRSSIVPFSLHKDQDQINIHSLSAKQKFISNLSLSMTNDQVIKKLFRSDSSVKKGGWVLDYSGPAEVLCNGHCNLIYSAILDENSDLLAKRRRNRFTIPLQSNQEKEQKPRSGISIEIPINGLFRINSILAYFEDPRYRRKSSGITKYGTIEVDSIVKKEDLIEYRRTKEFRPKYQMKIDRFFFIPEEVHILPGSSSIMIRNNSIIGVDTRITFNIRSQVGGLVRVEKKKKKIELKIFSGNIYFPGETDPISWHSGILIPPGAGKKNSKESKKLKNGIYVQRITPTK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+) binding.2. The protein should have zinc ion binding, DNA binding and be active in chloroplast, DNA-directed RNA polymerase complex.3. The protein requires Zn(2+) to activate a critical catalytic site. The designed protein sequence is ",
    "response#1": "GLTITDYLMSGYGARKGIVDTALKTANSGYLTRRLIDIAQDIVIREKDCLTSSSFLIDSQTKFDTEQLIGRVLLKSVYDSKTEKLIAKANTQLTLDLLNLFNQKQISKFYIRSPLTCNLYHSICQMCYGWDLSNQNLVDLGEAVGILAGQSIGEPGTQLTMRTFHTGGIFTSEARQQIISPKNGIIKFSKILKTIILRTNRGEDVLLTKNSGSLILIPEKHTDEIIQIELLRNTMLFVKSNQYIKQSAIIGELISTDKQTLTERKPILSDTAGEIFIPRLKTRMNLITQNRLLWILSGQVYQAPSNSFLNFYTDHKINKNSYIFRTKLINQYSGYTKVLNQNKNLLEQKIQITNETYFLD",
    "response#2": "MGMVLDCIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPEIIVNNEKRMLQEAVDALFDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKVMALELFKPFVMKRLVDLNHAQNIKSAERMVERATSGLSRYAMVWDVLEEVIKEHPVLLNRAPTLHRLGIQAFEPQLVEGKAIQIHPLVCAAFNADFDGDQMAVHLPLSAEAQAEARILMLSSNNILKPADGRPVTMPSQDMVFGIYYLL",
    "response#3": "GLTVTEYVISCYGARKGLVDTALRTADSGYLTRRLVDVAQHVIIRETDCGTLSGISLMPMKDQDKIIFSLEERLIGRVLAESIYSIQEKKYVARRNQDISANLATQIVRVKKNNIFVRSPLTCKANRSVCQLCYGWSLAHGSLVDLGEAVGIIAAQSIGEPGTQLTMRTFHTGGVFTGNVAKQIRSPIDGIVHFPTILQNQMTTTRTRHGEEALLTNIDVEILIQTNAEKSVQLKLPQYTVLFIKDGQFVRSKQLIAEVSSKNKKLQSVEIASKP"
  },
  {
    "reference": "MAKKLSRRFKEALAKVDQDKAYEPLEALNLLKETATAKFDETAEVHVRLGIDPKYTDQQLRTTVTFPKGTGQTVRVAVIARGEKVQEASSAGADIVGSEELIDEIQNGMMDFEVLIATPDMMPKVAKLGRLLGPRGLMPSPKGGTVTTDLAAAIQEFKAGKQEFRADRTGIVHVMFGKASFTAEDLLVNLKALQETIDRNRPSGAKGRYWRTVFVSASMGPSIQVDVNSLRDLKVTEA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design must be able to perform rRNA binding, structural constituent of ribosome in order to contribute to translation. The designed protein sequence is ",
    "response#1": "EGDVVKVIAGSHKGELGPITWISKDKKWVSVQGINSLKHVKPSQTDSEGGIKEVPSKINLSNVAY",
    "response#2": "MFLVGRIGKPHGIRGELTLDVRTDEPERRFAPGAVLRAEPPAGSASRLRRVTVRSARWHQSTLLVAFEEVADRTAAEAARGILLHR",
    "response#3": "METR"
  },
  {
    "reference": "MPLEGKTVLLGITGGIAAYKAADYSKKIRALGARVIPVMTRHATEFVSPITFAALTGEKVYTDLFCVEGAETIPHIELAGSADLFLVLPATANIIAKAANGLADDFLSTLLLAFSGPALFSPSMNPAMYAHPATQANIERLKTLDYRVIEPEIGETACGDRGRGRLPEWSVVREAILKATTPQTLRGMNVLVSAGPTKEYLDPVRYISNRSSGVMGYAMAMVAFRRGARVTLVSGSVSIPYPPGIKPCSVETAGEMEDIIGRLSPDAHVIIMTAAVADYTLAARADQKIKKDTSELRLDLVRTNDILSQLVRSRKGGQIIVGFCAETQDLKAQAIKKIRLKGADLLIANDVSQPDAGFDVPNNRVLIVSAGGDVEALPLLHKEEVGERVWDRIQDLLPQDNY",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.2. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.3. The protein should have a fast turnover rate for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. We require a protein that can specifically bind to the metabolites produced in the CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis pathway.5. The protein should be able to modulate pantothenate catabolic process in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "HIVLGLSGGIACYKAADLVRELVKAGATVQVVMTEAAEAFITPVTLQALSGRPVWTSQWDARAPNNMAHINVSREADAILIAPASADFMAKLAQGRADELLSLTCLARPFDTVPLLVAPAMNREMWAHPATQRNVAQLRQDGTTVLGPAQGDQACGETGDGRMLEPAELRDHIVLGLSGGIACYKAADLVRELVKAGATVQVVMTEAAEAFITPVTLQALSGRPVWTSQWDARAPNNMAHINVSREADAILIAPASADFMAKLAQGRADELLSLTCLARPFDTVPLLVAPAMNREMWAHPATQRNVAQLRQDGTTVLGPAQGDQACGETGDGRMLEPAELRDHIVLGLSGGIACYKAADLVRELVKAGATVQVVMTEAAEAFITPVTLQALSGRPVWTSQWDARAPNNMAHINVSREADAILIAPASADFMAKLAQGRADELLSLTCLARPFDTVPLLVAPAMNREMWAHPATQRNVAQLRQDGTTVLGPAQGDQACGETGDGRMLEPAELRDHLLAVLGTAPAPDLEGRRIVVTAGGTREALDPVRFIGNNSSGKQGVAFAAAAAARGADVTLIAANLEVHAPGGVTLVPVVSAEELAREVFAAARGADAVVMAAAVADYRPAERSSRKLKKEALGDGLTIRLVKNRDILSSLVLEKSVAGPIIGFAAETAEGEELLDLGRQKMARKKCDLLVINSVGGDEGFGSSTNTVTVLAADGSEVPVPRGSKWELSHRVLDAVVR",
    "response#2": "KRILLVIGGGIAAYKSLDLIRRLKERGAAVRVVMTSAAQHFVTPLAAGALSHDRVFTELFDREAEQDIGHIRLSREADLLIVAPATADLMAKMAHGLADDLASAVLLASTRPILLAPAMNPAMWANAATRRNAAQLAADGVRFVGPNAGAMAERGEDGAGRMAEPLEIVAAVAAALAPVADVVMLEGRRIVVTAGGTREALDPVRFIGNNSSGKQGVAFAAAAAARGADVTLIAANLEVHAPGGVTLVPVVSAEELAREVFAAARGADAVVMAAAVADYRPAERSSRKLKKEALGDGLTIRLVKNRDILSSLVLEKSVAGPIIGFAAETAEGEELLDLGRQKMARKKCDLLVINSVGGDEGFGSSTNTVTVIGRDGTVEPFARGEKALLADVVWDLVDG",
    "response#3": "HIVLGLSGGIACYKAADLVRELVKAGATVQVVMTEAAEAFITPVTLQALSGRPVWTSQWDARAPNNMAHINVSREADAILIAPASADFMAKLAQGRADELLSLTCLARPFDTVPLLVAPAMNREMWAHPATQRNVAQLRQDGTTVLGPAQGDQACGETGDGRMLEPAELRDEVVLGVGAGIAAYKVCDLLRRFTESGHGVTVVPTAEALRFVGEPTWAALSGRPVATGVWDGVHEVPHVRIGQRADLVFVAPATADLLARAAHGLAGDLLTNTLLTARCPVVFAPAMHTEMWEHPATQANVATLRSRGAIVLDPAVLAAGERTVGSGLEGRRIVVTAGGTREALDPVRFIGNNSSGKQGVAFAAAAAARGADVTLIAANLEVHAPGGVTLVPVVSAEELAREVFAAARGADAVVMAAAVADYRPAERSSRKLKKEALGDGLTIRLVKNRDILSSLVLEKSVAGPIIGFAAETAEGEELLDLGRQKMARKKCDLLVINSVGGDEGFGSSTNTVTVVGADGAVVDRVEGSKQVVADAVVGLVAAHL"
  },
  {
    "reference": "MNEMENTDPVLQDDLVSKYERELSTEQEEDTPVILTQLNEDGTTSNYFDKRKLKIAPRSTLQFKVGPPFELVRDYCPVVESHTGRTLDLRIIPRIDRGFDHIDEEWVGYKRNYFTLVSTFETANCDLDTFLKSSFDLLVEDSSVESRLRVQYFAIKIKAKNDDDDTEINLVQHTAKRDKGPQFCPSVCPLVPSPLPKHQIIREASNVRNITKMKKYDSTFYLHRDHVNYEEYGVDSLLFSYPEDSIQKVARYERVQFASSISVKKPSQQNKHFSLHVILGAVVDPDTFHGENPGIPYDELALKNGSKGMFVYLQEMKTPPLIIRGRSPSNYASSQRITVRTPSSVNSSQNSTKRKMPSMAQPLNESCLNARPSKRRSKVALGAPNSGASISPIKSRQSTPMEASKENEDPFFRPNKRVETLEHIQNKLGALKNQCPDSSLKYPSSSSRGMEGCLEKEDLVYSSSFSVNMKQIELKPARSFEHENIFKVGSLAFKKINELPHENYDITIEKKSMEQNYLRPEIGSRSECKTSYGNELSLSNISFSILPNSAENFHLETALFPATEEDVPRTFSRILETGSFQNYYQKMDAENADRVYSKGVKLIASGTLPSGIFNREELFEEDSFYKY",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The engineered protein is expected to adopt a strand-strand-turn-helix-strand-strand-turn-strand-strand-strand-strand-helix-strand-turn-strand-based conformation.2. For general function, the protein need meet that Transcription factor required for successful completion of meiosis and spore formation3. A protein with DNA-binding transcription factor activity functions is required to enable meiotic cell cycle. The designed protein sequence is ",
    "response#1": "AEVNIGMVGHVDHGKTTLTKALTGVWTDTHSEELRRGITIKIGFADAEIRRCPNCGRYSTSPVCPYCGHETEFVRRVSFIDAPGHEALMTTMLAGASLMDGAILVIAANEPCPRPQTREHLMALQIIGQKNIIIAQNKIELVDKEKALENYRQIKEFIEGTVAENAPIIPISALHGANIDVLVKAIQAEVNIGMVGHVDHGKTTLTKALTGVWTDTHSEELRRGITIKIGFADAEIRRCPNCGRYSTSPVCPYCGHETEFVRRVSFIDAPGHEALMTTMLAGASLMDGAILVIAANEPCPRPQTREHLMALQIIGQKNIIIAQNKIELVDKEKALENYRQIKEFIEGTVAENAPIIPISALHGANIDVLVKAIEDFIPTPGGVLGGSIVQGKLKVGDEIEIRPGVPYEEHGRIKYEPITTEIVSLQAGGQFVEEAYPGGLVGVGTKLDPYLTKGDLMAGNAAPPVWDSLRLEVHLLERVVGTEQELKVEPIKRKEVLLLNVGTARTMGLVTGLGKDEIEVKLQIPVCAEPGDRVAISRQIGSRWRLIGYGIIRHRNELNHTLAQLPLPAKRVMYMALAPIDSKEPLERGRVFKIRAEDLAALAKITPSLAYRQLKEGGKLLGASKISLRGDDIIALAKELNLPFTAKNSPEELDLNIIEWIAYSPDEGYLSLKFTRTIEPYISSLIGKKNKFTTQLLTASLRLSSQYSSSLYQLSEDR",
    "response#2": "IRHRNELNHTLAQLPLPAKRVMYMALAPIDSKEPLERGRVFKIRAEDLAALAKITPSLAYRQLKEGGKLLGASKISLRGDDIIALAKELNLPFTAKNSPEELDLNIIEWIAYSPDEGYLSLKFTRTIEPYISSLIGKKNKFTTQLLTASLRLSSQYSSSLYQLL",
    "response#3": "VNIGMVGHVDHGKTTLTKALTGVWTDTHSEELRRGITIKIGFADAEIRRCPNCGRYSTSPVCPYCGHETEFVRRVSFIDAPGHEALMTTMLAGASLMDGAILVIAANEPCPRPQTREHLMALQIIGQKNIIIAQNKIELVDKEKALENYRQIKEFIEGTVAENAPIIPISALHGANIDVLVKAIEDFIPTPKLKTPVRQLLLASENPQQFMDYFSEEFRNDFLELLRRRFGTKRVHNNIVYNEYISHREHIHMNATQWETLTDFTKWLGREGLCKVDETPKGWYIQYIDRDPETIRRQLELEKKKKQDLDDEEKTAKFIEEQVRHYWEVDVGTSQVWDVGVCKESVNRQGKIVLSSEHGFLTVGCREGKVFAASTVPMTPLWVSPQLHRVGIFLDVGMRSIAFYNVSDGCHIYTFIEIPVCEPWRPFFAHKRI"
  },
  {
    "reference": "MSNKVDITSLNFDELEDLVTDLGEASFRAKQIANWTYKKGAIDFDGMTNLNQELQDKLNEVAYISQLKEITRAESKDGTIKFLFELIDGKRIETVLMPYKDGRNSVCVSTQVGCGMGCNFCATGLQGLERNLTTGEIVGQVLTVQRLISKGEFGTPPVTNIVFMGMGEPLANYDNLLSAINLLNDEKGLNISMRRMTISTCGLVPKIEKLADEQLQVVLAISLHAANDRLRSDMMPINEEYPLSELVGACQYYIEKTNRRVTFEYALIDGVNNKPKDAKSLANLLSGLLCHVNLIPINPVDELELVRPDRRDIKRFKDVLSKSGIEATVRVERGKDIDAACGQLRAKQDN",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The [4Fe-4S] cluster, S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand.2. The designed protein must be optimized for rRNA (adenine-C2-)-methyltransferase activity.3. The desired protein should be able to perform adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine4. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.. The designed protein sequence is ",
    "response#1": "EDDRGTLCVSSQAGCAVGCRFCSTGHQGFSRNLSTAEIVAQLWFAEHFLRKHLARDERIISNVVMMGMGEPLQNYAALVPALRTMLDDNAYGLSRRRVTVSTSGVVPMIDRLAQDCPVAMAVSLHAPNDLLRDDLVPLNRKYPIAELLAACKRYLEHAPRDFITFEYCMLDGVNDSDAHAQQLIDLVRSQDVSCKFNLIPFNPFPASGLLRSPAQRVQAFARKLADAGIITTVRKTRGDDIAAACGQLAGGYREWSSVCLSTQVGCPMACAFCATGRGGFARNLSAGEIVAQFLLARAHAPCPVTHAVFMGMGEPLLNTSAVLQALHLLNSECGIGARNLTLSTVGIVPGIQRLAREKLQINLALSLHAPDDETRARFMPVAQHFPLPVVLAALRDYTAATHRRVTLEYLLIAGVNDSPAHADRLADLVLGKKEEGGPLLAVVNLIPYNETEARFRRPSEEGIRRFMARLKQRGVAVTRRLERGEEADAACGQLA",
    "response#2": "DEKRRTLCVSSQVGCSLDCSFCSTGKQGFQRNLTPAEIIGQVWVAQRSFGPRTDTTKRPVTNVVMMGMGEPLMNFEPVMSAMRLMLDDFGYGLSKRRVTLSTSGVVPAIEKMTGQIDVALAISLHAPNDELRNELVPINRKYPIKMLLEACNRYLDTLGDNRSITIEYTLIAGVNDQPQHAAQLAKVLRTLDCKINLIPFNPFPNNDYQRPSRNAIVRFQTAMIDAGYVVTVRSTRGDDIDAACGQLVGSEVDRGTLCVSSQVGCTLSCTFCHTGTQRLVRNLTTEEIVAQLMVARDRLGDFPGRTPPEGAFVPNDGGRFVSNIVFMGMGEPLYNFDGVRDAIDVISDNEGLSLGRRRITVSTSGVVPQIEALGAEMGTMLAISLHAVRDDLRNELVPLNKKYPIRDLLEACRNYPGLSNAKRITFEYVMLKGVNDSDAEARELVRLLKGIPAKINLIPFNPWPGTRYECSDWDRIERFSEIVYRAGYASPVRTPRGRDIIAACGQLRG",
    "response#3": "DGDRATLCVSSQVGCKMGCAFCQTGKQGFDGNLTAGDILNQIYALPEREQLTNIVMMGQGEPMDNVENVLAAIEVMTASWGYGWSPKRITVSTVGLRKGLRKFLNGCDCHLAVSLHSPIHEQRLNMMPAEGAMTIDEVVETLREYDFSHQRRLSFEYIVFKGLNDSRMHAKEIVKLLSGLDCRINLIRFHEIPGVDLPSSDEEGMLRLRDYLTAHGFFTTIPWHRLDYDAEKKHLLGMTLDELKQVVSDNGLPAFAAKQIADWIYKKKISSVDEMTNISVANRNVLAEKYDIGRNVPIEFQKSTDGTKKYLFRNDSGKYIESVMIPDDDRKTLCVSSQIGCKLNCLFCMTGKQGFNGNLTTNEIINQIYSVEEADKLTNVVFMGMGEPLDNYEELSKALEIMTADYGMAWSPKRITVSTTGIIPKLKLFLEESNAHLAVSLHSPFREQRSSIMPVEKAFPLSDILNLLREYDWSHQRRLSFEYIMFKDFNDSVIYAKELANILRGLDCRVNLIRFHAIPGVDLETSRNDVMEKFRDYLVGKGVNCTIRSSRGEDIFAACGMLSTEKNSDLFID"
  },
  {
    "reference": "MYTEINEMLTPKVLKVQAESPYKARIVLEPLERGFGHTLGNALRRILLSSMPGSAITEASIDGVLHEYSTIEGVQEDVVDLLLNLKSVAIKLTVGNEAQVTLNKEGPCQVTAGDIQLTHGQEIINPELVIANLNEKGKLNMTLKVERGIGFHNTDAFVRYHDDEIEKKTVGKLKIDNSFSPVKKVAYFVDSARVENRTDLDKLTIELETNGTIDAEEAIRISASILQRQLHAFVDMKFEESRADNKERNDFDPVLLRSVDDLELTVRSANCLKAENIHYIGDLVQRTESELLKTPNLGKKSLTEIKDVLASRSLSLGMKLENWPPASLGE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have protein dimerization activity within DNA-directed RNA polymerase complex to regulate DNA-templated transcription.2. The protein should have a low Km value for a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +   RNA(n+1).3. The protein must exhibit the following characteristics: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.. The designed protein sequence is ",
    "response#1": "LIEVQKASYDQFLMIEEPKGGRPDEGLQAVFKSVFPISDFSGTAMLEFVGYQFDSPKFDVEECRQRDLTYAAPLKVILRLIVFDVDEDTGSKDIKDIKEQGVYMGDMPLMTTNGTFIVNGTERVIVSQMHRSPGVFFDHDKGKSHSSGKFLFAARVIPYRGSWLDIEFDAKDIIYARIDRRRKIPVTSLLMALGMDASDILSTFYNKVTYERDGDGWRIPYSVDRFKGMKLVSDLIDADSGEVVAEAGKKLTVRAAKALAEKGLKAVKVSEDDLLGSYLAEDIVNYQTGEIYLEAGDEIDEKTLRVLFDVNADQIDILDIDHMNIGAYIRNTLKVDKNESRQDALFDIYRVMRPGEPPTMDTAEAMFHSLFFDPERYDLSAVGRVKMNLRMDLDCPDTVRVLRQEDILAVVKMLVELRDGRGEIDDIDNLGNRRVRSVGELMENQYRIGLLRMERAIKERMSSVEIDTVMPQDLINAKFAAH",
    "response#2": "EWMMLDVIPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLQELNAPDIIIRNEKRMLQEAVDALFDNGRRGKTITGPNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELRLHQCGLPKIMALELFKPFIYNKLEEKGYVTTIKSAKKMVEKERPEVWDILEDVIREHPVLLNRAPTLHRLGMQAFEPVLIEGKAIQLHPLVCAAFNADFDGDQMAVHVPLSIEAQMEARVLMMSTNNILSPANGKPIIVPTQDMVLGIYYML",
    "response#3": "RSPGVIFKEEESPTVVNKLIYTAQIIPDRGSWLYFEYDTKDVLYVRINKRRKVPITILFRALGYKKQDIVKLFD"
  },
  {
    "reference": "MSLSGKRILLIVSGGIAAYKALDLIRRLRERGAEVRPLMTAGAQEFITPLAVGALSSGHVYTELFSREDEQDVGHIRLARDCDLVCVAPATADLMAKMAHGLANDLASTVLLATDRPVLVAPAMNPAMWAHPATKRNVETLRRDGIRFIGPASGEMAESGEAGTGRMAEPLDIVASIAEILAPAAKPLAGRRAIVTSGPTHEPIDPVRYIANRSSGKQGHAIAAALARLGAEVTLVSGPVSIPDPAGVHAIHVESARDMLDAVTDALPADIAVFVAAVADWRVATSAENKIKKKEGEGPAPLELTENPDILRTIGHHAMRPALVVGFAAETADVISNAQAKLARKGADFIVANDVSPATGIMGGDRNRVRIVSKSGVEDWPDLSKAEVAERLAARIAELVPKMAS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein requires Mg(2+) to activate a critical catalytic site.2. The protein design must be able to perform metal ion binding, phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity, FMN binding in order to contribute to coenzyme A biosynthetic process, pantothenate catabolic process.3. The designed protein should contain one or more DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains that are essential for its function.4. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..5. The protein design should consider the endogenous proteins and enzymes present in the coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis pathway to avoid interference with native metabolic processes.6. The protein should have a low Km value for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.7. The CTP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "KKILIAISGGIAAYKTHFLIRDFVKKGAEVQIIMTPDAEHFVTKLSISTLSKKPVYTDFYSDNGTWNSHVEIALWADVMIVAPCTANTLAKMVHGMCDNLVIATYMSAKCPVFIAPAMDLDMYAHPSTNKNLVLAESYGHIVIPAENGELASGLTGQGRMAEPETISKAVESGVLAQSILEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLLEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLLYDDNGGLEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLPPHSNPEVVLEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVL",
    "response#2": "TIILGVTGGIAIHKSLDVASQLVKSGASVHVVMTENATRLVQPLQFQVISRNPVLLDLFDTGTDWKPPHIDLADRADLLMIVPATANIIGKLANGIADDALSTVAVSVHCPVLLAPAMNGHMYQNPFVQQNINTLKTHNYHFIEPASGDLACGYEGVGRLNTVETILEESRIRDTVLAAVETALTPLAGRRAVVTSGPTVEAIDPVRFIANRSSGKQGHAVAAALAALGAEVTLVSGPVTIPDPAGVRMVRVESARDMLAACQAALPADILVCAAAVADWRVEAADQKLKKQPGGAPPALVLSENPDILATLSQAGARRPRLVVGFAAETETVVEHAVAKRRRKGCDWILANDVSPATGTFGGDDNTLHLELLATTESVVWAPAAELSKAQLAFALIDRIAALKPRI",
    "response#3": "TIILGVTGGIAIHKSLDVASQLVKSGASVHVVMTENATRLVQPLQFQVISRNPVLLDLFDTGTDWKPPHIDLADRADLLMIVPATANIIGKLANGIADDALSTVAVSVHCPVLLAPAMNGHMYQNPFVQQNINTLKTHNYHFIEPASGDLACGYEGVGRLNTVETILETLEAKDALSDFLEGVRLVVATGGTREHLDPVRFLGNSSSGLMGLSIARAAALRGAEVTVVAAHVEHPLPSGASIRRIVSTGDLDEAMTELAPSADVIVMAVAAADFTPTEVSEGKIKKADDSTGLSLDLVQTPDVLKHISHLPDRDPGQVIVGFAAETAGDQTALLELGQAKLARKGCDLLVLNDVSAGAVFGAADNHVLSVLEDGKLVLQGIRVLVTAGATREYIDPVRFITNRSSGKMGYAIAEAAAHRGAEVRLISGAATLPPPTHIKTQHVETTIQMYNEVLQAFDETDIVIMAAAPADYRPREFTPHKIKKTADPLTLPLERNPDIAEALGKQKTHQILVCFAAETDDLLENAKKKLIRKNCDLIVANDILAEGAGFQSDTNIVTLLTKGKEKLVLPMAPKLEIAHHLLDYVADKLS"
  },
  {
    "reference": "MKKYQRYLELDRSQQQNFLYPLIFREYIYGLAPSHDLNRNRYILSENVDYDKNSSLLIVKRLITRIYQQNHLILFDNDSSKNQFWGYNKNLYSQIISEGFAIVMEIPFSRQLSSSLEEAGIVKSFNNLESIHSIFSFFEDKFTYLKFFSDVRIPYPIHLEILVQTXXXXXXXXPLLHLLRLFLYEYCNWTSLITQKKIIFTFSKSNPRFFLFLYNFYVCEHESIFLFLRKKSSHLRLNSFSVFFERIYFYTKLEHLVEVFSKNFSSTLSFFKDPLIHYVRYQGKSIFASKNAPFLMNKWKYYFIYFWQCYFDIWSQPRMIQINELFENSFHFFWGGYLSNVRLNFSVVRSQMLEDSFLIEIVMKKLDTIVPIIPLIRSLAKAKFCNRLGHPISKPVWTDSSDFDIIDRYLRICRNLSHYYNGSSKKSLYRIKYIIRLSCIKTLARKHKRTLRAFLKRLDSEELLEEFFTEEEEILSLIFPRSSATLRRLYRSRIWYLDILFSNDTINVN",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be involved in tRNA processing by localizing to chloroplast and exhibiting RNA binding. The designed protein sequence is ",
    "response#1": "MKKLDTIVPIIPLIGSLAKTKFCNAVGHPISKPIRADSADSDIIDRFVRICRNLSHYYSGSSKKKSLYRIKYILRLSCVKTLARKHKSTVRAFLKRLGSELLEEFFTEEEQILSLIFPKVSSG",
    "response#2": "ESRAKAGQHLQDFLPLLLRLFSDEYDEVCSTVIPSLTDILTFLRKVPELPDSYREMLRPIMDAIVAKMRFDETSHWFEGEEAEEEADFLELRKRLQNLQKSVAAVDENLFIDVMSNLVATTLQNLDERGAQMDWRDIDLALHELLQFGELALPNQGLAAKSQPSSNAAERLNVIMRKLVESSIADFPHPAVLLQYMEVCVRYCVFFETNHSYIPRVLENFVRLVHYDLTRFRVRSWYLFHRFVKQLRAQVGNVAETIIQSIADLLPIKAEVSAEDGSEDDMSSDQTDNSADAIFNSQLYLFEAIGYISSTSATPVDKQALYARSVMEPLFRDMENHLEKAKSGDAQAILQIHHVIMALGTLAHGFGDHAKPGHQRQAPDKLVSAEFARAAEAILIALGQLNSRMDIRAACRSAFSKLLNVLGSAVV",
    "response#3": "ESRAKAGQHLQDFLPLLLRLFSDEYDEVCSTVIPSLTDILTFLRKVPELPDSYREMLRPIMDAIVAKMRFDETSHWFEGEEAEEEADFLELRKRLQNLQKSVAAVDENLFIDVMSNLVATTLQNLDERGAQMDWRDIDLALHELLQFGELALPNQGLAAKSQPSSNAAERLNVIMRKLVESSIADFPHPAVLLQYMEVCVRYCVFFETNHSYIPRVLENFVRLVHYDLTRFRVRSWYLFHRFVKQLRAQVGNVAETIIQSIADLLPIKAEVSAEDGSEDDMSSDQTDNSADAIFNSQLYLFEAIGYISSTSATPVDKQALYARSVMEPLFRDMENHLEKAKSGDAQAILQIHHVIMALGTLAHGFGDHAKPGHQRQAPDKLVSAEFARAAEAILIALGQLNSRMDIRAACRSAFSKLLNVLGSAVL"
  },
  {
    "reference": "MKPNLLGLSRDKMKELFSELGEXQFRAQQVMQWVHQRGVLNLGEMTNISKSLRGNLEKLVEIRVPKIVGHYPSEDGSHKWIIEVVSGSRVEMVFIPEGSRGTLCVSSQAGCSLDCRFCATGKQGFNSNLTVAEIVGQVWLAKQQLDGFDAASQRSVSNVVMMGMGEPLMNFEPVMDAVNIMMDDWAYGLSKRKVTISTSGVVPAIRRMKEYTDASIAISLHAPNDALRSSIMPINKSYSIADLLDAVKEYADSLNDRRVPVIEYTVIKGVNDHRQHARELAELLKNLPCKINLIPFNPFSMSEYRRPSDSVINNFREILQRAGYTVTVRTTRGGDIGAACGQLVGEVEDKTRRKERHILRAEARQNEEARSSNGIDEERAEILASTGKVKTLDDRLTLGP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be able to perform tRNA binding, rRNA binding under specific conditions.2. The Proton acceptor active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "WTVCLSTQVGCKFRCAFCASGLKGFKRNLSTGEIVSQVLTLKYRLGHPVTNLVFMGMGEPLDNLENVVKAIKILNDKQGLDLGARRMTVSTAGVIPGIKKLKDLGLQINLSLSIHSALDRQRTLLMPINKKYPLAELVAAAEDYLKGGGRQLTLEYIVIKNLNDGLYEAEGLATLARRLRAKVNLIAYSPVPELPYARPTPAEVSRFEAY",
    "response#2": "CKRYMGRVIKNVKVQESPDWLKSRIRAMGLNPINNIVDITNFVMFEYNQPMHAFDLDKLEGNITIRAAKKNEEITTLDGVDRVLKNGELVIADDEKAIAIAGVIGGQNTQIDNETKNIFVEVAYFTPENIRKTSRELGIFTDSAYRNERGMDVENLSNVMARAVSLIAEVAE",
    "response#3": "WTVCLSTQVGCKFRCAFCASGLKGFKRNLSTGEIVSQVLTLKYRLGHPVTNLVFMGMGEPLDNLENVVKAIKILNDKQGLDLGARRMTVSTAGVIPGIKKLKDLGLQINLSLSIHSALDRQRTLLMPINKKYPLAELVAAAEDYLKGGGRQLTLEYIVIKNLNDGLYEAEGLATLARRLRAKVNLIAYSPVPELPYARPTPAEVSRFEAR"
  },
  {
    "reference": "GQMPLHMRLPKLKGFKNPFKTEFQVVNLDKLAALYPEGGEVTVADLVAKGAVRKNSLVKVLGQGEISVALQVTVDAVSGSAKEKITAAGGTVTELV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must have high specificity towards rRNA binding.2. For general function, the protein need meet that Binds to the 23S rRNA. The designed protein sequence is ",
    "response#1": "SDTALRHXPIGANQSAFKSKRXASESLASELIAASNRDAKCFSINRKDGKERVAKAAL",
    "response#2": "MAHDTALRHXPIGANQSAFKSKRXASESLASELIAASNRDAKCFSINRKDGKERVAKAAA",
    "response#3": "TASELAKSFTEIDVLPIYAQAERGVLFGAVSAKQVVAELSKKGIEITTKNVVLGAPIKALGEHEVRIFLHSRVECSLKIHF"
  },
  {
    "reference": "MYAVIKTGGKQYKVAEGEFLKVEKLVGEVGDSVEFGEVLMIGGEKIVVGAPHVAGATVTAKIAVQGKDKKILVFKSKRRKGTRKLRGHRQHKTVLKIEKISA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be involved in translation by localizing to ribonucleoprotein complex, ribosome and exhibiting structural constituent of ribosome, rRNA binding.2. For general function, the protein need meet that This protein binds to 23S rRNA in the presence of protein L20. The designed protein sequence is ",
    "response#1": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTF",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#3": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG"
  },
  {
    "reference": "MAINTKKPVARRANNMLTRTNKRVTDAMAPVKKIFNEVLTGRGIVRAFLALLMFMRFLSIKPPKHMTERWKKVKKSEGLKILKGFKSDIGTKLNQLNKRVSKRRGSASLMPLMWGVASAVTFTTFAGFPLMTVEPSDIGKAIAVPTPSGNVTCYVQANTVGEMCDHSVTYLCPKVDVEREDVDCWCHGGEAYVRYGKCRRGKNRAQRSRRSVGISAHGSGGLAHKKTRWMSVDASMEHFQRTERWVLRNPGYALIAGLMGWMLGSNRTQKVMFIILLLLVIPAYSMKCIGVQNRDFITGVKGTTWVDVALEAGGCVTITAQDKPTMDIIFTQSVAKKPAHVRKVCLEASITEISHVATCPTNGDAHNPKAKDTLFMCKRELTDRGWGNGCAFFAKGSLESCCKFACKKSYDASVITRENIEHALEVHIHTGKELYHHGNDSQFAKAKTGAVVNFSPKASEQTVDLGDYGTLGLVCRAEGGVEYETSYVFGRKDNNKYANGWLANRMWVDDLPLPWTSATSDLWHNREALVDFGETHAVERSVVVLGDQEGMLMKALAGATTLDFFEEADTVGVIAGHLTCRIKVENLKIKGATYPVCPNTVVLTKEPVDTGHSTVVVEVKLSTFTMACRLAVTFTDAAGTKITGRLITVNPIITAADEKVTIEMEPPFGESFIEIGVTADSVKHHWNRKGSSIGDAFYATYRGARRMAVLGDAAWDFNSIGGAFNSIGKGVHAMFGKVFTVLFGGLSWISQMAIGALLIWLGVGARNK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must have at least one Peptidase S7, Helicase C-terminal, Flavivirus NS2B domain.2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.3. The designed protein should have protein dimerization activity, RNA-dependent RNA polymerase activity, serine-type endopeptidase activity, RNA helicase activity, mRNA (guanine-N7-)-methyltransferase activity within virion membrane, membrane, host cell endoplasmic reticulum membrane, extracellular region, viral capsid to regulate viral entry into host cell, suppression by virus of host type I interferon-mediated signaling pathway, viral RNA genome replication, virion attachment to host cell, suppression by virus of host JAK-STAT cascade via inhibition of STAT2 activity.4. The designed protein must have a Charge relay system; for serine protease NS3 activity that is structurally stable and resistant to denaturation.5. The protein should have a high affinity for Zn(2+).6. For general function, the protein need meet that Serine protease subunit NS2B: Required cofactor for the serine protease function of NS3. The designed protein sequence is ",
    "response#1": "HCTNIENRDFLSGAQGTTWTSVVLEHGGCVTMTSEEKPSLDIWLESVTISDPPLVRKICYNAHLSDQKTANKCPTTGEALLDEENEVDFECKRGFSDRGWGSGCGLFGKGSIVSCAKFTCQNSMHLYEIGQDKVHFSIRMQLHTSERDSTSADWKRSLEFNLLSGQQTITFSGYGTVTVSCSLRTTVDLNNYYIATVGTQLFLVNKHWATDLPLPWTPASGDAWREKHYLTHFEEPHATSVTVSTLGDQEGAVKHALSGATRLQLVSGKYVLKGGHAVCQVQTHGLSLKXGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGGLPAFTFARTPSETIHGTATVELQYAGEDGPCKVPIVITSDTNSMASTGRLITANPVVTESGANSKMMVEIDPPFGDSYIIVGTGTTKITHHWHRELEDGAYRIKQRGILGYSQIGAGVYKEGTFHTMWHVTRGAVLMHRGGRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLK",
    "response#2": "RCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKXXLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVHCTNIENRDFLSGAQGTTWTSVVLEHGGCVTMTSEEKPSLDIWLESVTISDPPLVRKICYNAHLSDQKTANKCPTTGEALLDEENEVDFECKRGFSDRGWGSGCGLFGKGSIVSCAKFTCQNSMHLYEIGQDKVHFSIRMQLHTSERDSTSADWKRSLEFNLLSGQQTITFSGYGTVTVSCSLRTTVDLNNYYIATVGTQLFLVNKHWATDLPLPWTPASGDAWREKHYLTHFEEPHATSVTVSTLGDQEGAVKHALSGATRLQLVSGKYVLKGGHAVCQVQTHGLSLKG",
    "response#3": "RCIGVENRDFIEGISGGTWVDLVLEHGGCVTVRAEGKPTLDIELTNSFATGMAETRKYCYECEVSKITTVNHCPQMGEAHNSASSDPKQVCKKGYSDRGWNNGCGLFGKGSLETCAEFACKKEAPGHVIRKENLEYNVMVAVHGSVPSENAGNDTSHAVKLSFTTASPVATGKLGDFGEVSMECEPRSGLDFEQYYMFTIDKNSWLVDRSWFHDLNLPWKGGSAGDWQNKQSMVEFAEPHATKQDVLALGSQEGALHSALGGAIPMKGTSSKVDITAGHLKCRLKMDKLLIKXRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGV"
  },
  {
    "reference": "MKKLALFALLMVPAILLASGHDSGEASRYLTQTGRESDFWPRVINFTIFAAILYYLIANPIKNFFKGRREGIAGQLKEIESKLQAAKDEKKEAQSHLNESVNKAAEIIEDAKKEAEILAAKIAEASENELVVLEKQFEEKITLEERKAARDVIDEVLSENITTDDIALDETKVVDIISRKAEHGKVA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0)..2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. The protein should have proton-transporting ATP synthase activity, rotational mechanism and be active in proton-transporting ATP synthase complex, coupling factor F(o), plasma membrane. The designed protein sequence is ",
    "response#1": "EAPSLLDIV",
    "response#2": "GSSGN",
    "response#3": "GHNS"
  },
  {
    "reference": "MSDVRIPGVGAGKYDNLIQSLMKKERIPRDNAAAKVKVYEVQNNALKDVERYARDLRDAVKGLYSFNNPFAEKEAHSSNERAFTVDATRDAAEQNHTLRVKDIAQGDAFLSDPLPEDFRVPSGTYTFCIGEKKICVSWKGGHYRDFIRAVNKQGKDSLTLSEIKTSGASRALLFRSELTGKSSRLSFEDAALDLALRLRVVQEARSDVFTQDVLSVGPGKHARLDFPHPLRAQAGLTLEFVASLEGASIANEESRAHTPAQGGAPTSSHGNTASAAHNQDGAAAVRPTEPANGAPVQEETSSVFFEGVTVKNEASQGDLPTTDGLEKYPAVDDKGDNPRAPGESQGTATHEGSGSSTDNADDTRSTGALAGSGKLALESLQGHALPLPPLVLTQNAPQMVSIPLREYGDVRALILDNAQARGALTLRAIRVRAEDAPGGYVPVNPASQAQDAAFDFDGVHVTRGTNSITDLIPGVTLSLHERTEKTETLSVTPDVNAMKNAIIEFVAKYNRLMAEINIVTSNKSAIIDELAYLTPEEKKKETEQLGSLHGDSTLLMLKDRLRRNTSNAYRAGDDGASRTLAHIGISTKAHASSGINTAQLRGYLEIDEEKLHSSLNAQKDQVRALFGHDSDGDLLVDNGVAFTLTELLNPYLGRSGIFAIRSNGVDERIKSTEKRVETYDKQLEKKERELRHKYHTMDGALRSLQKQSDAIQNFNQSVRNRN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should exhibit Polar residues compositional bias.2. The protein must exhibit the following characteristics: Forms a capping structure, which prevents flagellin subunits (transported through the central channel of the flagellum) from leaking out without polymerization at the distal end .; Required for the morphogenesis and for the elongation of the flagellar filament by facilitating polymerization of the flagellin monomers at the tip of growing filament.3. Design the protein with a signal peptide incorporated.4. The designed protein localizes to the bacterial-type flagellum hook. The designed protein sequence is ",
    "response#1": "AENAKIKFDGVDVERDSNVINDLVPNVTLSLKKPSSDMVEAKIEPDYEGIKRVLLDFIGAYNEVLAEINIVSSNEDQPNNQKSNIVEELTYLSDSQKEEAYKNLGILRSEFLLKNLKSKLESIIFKPYVTSDPNFSIINQMGVFTNSISSSGGLSRYLRLDEKKFDESIRNNIDNVRELFLYDLNGDRVYDNGIAKMLGDCLSPLVASGGVIYNKIKNYDLKIFNQKNKVEDYKKKYEDRERKVEGELNTLDFTVKRMR",
    "response#2": "AENAKIKFDGVDVERDSNVINDLVPNVTLSLKKPSSDMVEAKIEPDYEGIKRVLLDFIGAYNEVLAEINIVSSNEDQPNNQKSNIVEELTYLSDSQKEEAYKNLGILRSEFLLKNLKSKLESIIFKPYVTSDPNFSIINQMGVFTNSISSSGGLSRYLRLDEKKFDESIRNNIDNVRELFLYDLNGDRVYDNGIAKMLGDCLSPLVASGGVIYNKIKNYDLKIFNQKNKVEDYKKKYEDRERKVEGELNTLDFTVKRMK",
    "response#3": "TLAANNTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGYSVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTTDAEC"
  },
  {
    "reference": "MLDSQVIATIKSTIPLIVSTGPKLTAHFYERMFKHHPELKDIFNMSHQLNGDQREALFNALCAYAANLDNLSALLPAVEKIAHKHASLNIKPEHYQIVGTHLLATIDEMFHPGDEVLSAWGKAYNVLANVFINREEEIYQSGESLEGGWRELRQFRVSRKQPQSDVITSFEFVPVDGEKVMDYKPGQYLGIYIEDPAFENREIRQYSLTAAPNGSNYQIAIKRESQGKVSNFMHDKVQEGDLVMLAAPRGDFFLDVQHDTPVTLISAGVGLTPMMSMLQHLHNQHHTGTVNWFHAAEHGGYHAFSNKVNEISQSMPNLHPQVWYREPRDIDQKGIHYQHTGLMDLSLVKELIAAEGMQFYFCGPVAFMQFIANQLLAMGVDKQHIHYECFGPHKVI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a binding site that is specific to the target heme b, NADP(+), FAD ligand.2. The protein must exhibit the following characteristics: Therefore, plays a central role in the inducible response to nitrosative stress..3. The designed protein have Globin family profile, FAD-binding FR-type domains with specific sequence motifs that are crucial for its activity.4. The designed protein should have oxygen carrier activity, oxygen binding, FAD binding, nitric oxide dioxygenase activity to facilitate response to nitrosative stress, response to toxic substance.5. The protein's Charge relay system must be carefully engineered to ensure that the amino acids involved in NADPH + 2 nitric oxide + 2 O2 = H(+) + NADP(+) + 2 nitrate are properly positioned for optimal catalysis. The designed protein sequence is ",
    "response#1": "MLDSQTIATVKATIPVLAKTGPALTAHFYDRMFHHNPELKDVFNLTHQSSGAQREALFNAICAYAVNIENLAVLLPAVERIAQKHASFSIQPEQYAIVGHHLLATIDEMLSPGQEVLDAWGKAYQVLADVFINVEDQFYDNPTAVEGLRPFRIVKKQQESEIITSFELEPVDGKPVADFIPGQYIALYLQSDSLEYQEIRQYSLTHAPNGKYYRIAVKREPQGKASNYLHDVAQQGDILKLAPPYGDFHLDSAGKTPIVLLSGGAGITPMVSMLNTLVQQDSDRQIHWLHAAQNGELHAMKETVNQLAAQFKQLNVWYCYESPQEGDLMAYDHEGLIDLHALKEKVLPENAYLCGPLPFMQYVAKQLEQWGVPYEHIHYEFFGPAASLEAA",
    "response#2": "MLDSQTIATVKATIPVLAKTGPALTAHFYDRMFHHNPELKDVFNLTHQSSGAQREALFNAICAYAVNIENLAVLLPAVERIAQKHASFSIQPEQYAIVGHHLLATIDEMLSPGQEVLDAWGKAYQVLADVFINVEPSLYRQKVENGWRNFRIESKQQESELITSFVLRPKDDQPVLRHQPGQYLTFWIEVPGQHPLKRNYSISSAPNDESYRISVKREPQGIASNWLHDQAAVGQMVKVAPPAGEFFLEAEEQRLVMLAGGVGVTPLLSMAKSLVESRPDRQVVFLHAATNGRVHAMRQQVHELARQYPQLSVHYCYSEPTEADRQGEDFDKEGYIDLAWLKQLLPACTQFYLCGPVPFMAVINRALQELGVATDRIHYELFGPHEEM",
    "response#3": "MLSQQTIDVIQSTVPVLEVHGEAITRHFYQTLFTAHPELLNIFNHANQKQGRQQAALANMVYTAALHIDNLAAILPAVRQVAHKHRSLGIVPEQYAIVGTYLLQAIGDVLGEAATDEILAAWGDAYQVIADAFIGIEDSMLEGTRAFQIVSKTPQSALITAFEFAPQDGGDVADYQPGQYLGVWLKPEGFAHQEIRQYSLTRSPNAKNYRIAVKRENGGQVSEWLHDQARVGDTVYLAAPAGDFFMDTESRPVYLISGGVGITPMMSMLKRVIQEFQPGQYVTVREGQRPFRIEKKQRESDVITSFTLVPVDGLPISDFRPGQYVAVYIRSDALEYQEIRQYSLTHAPNGHSYRIAVRREDEGKVSRYLHDTAQQGDVIHLAPPYGDFWLEELIEAKPDSQFYLCGPVPFMRAVNQALLAKDVPEERIHFEFFGPAGSLE"
  },
  {
    "reference": "MLVFLILLPFLLLVIFGAYLLVCLFVRQNNLLLKKTKQLYNNLKLSNFNCVISPFQLLRNEKQALEQSIKVLKQFQIKFDNEINSAFEQLTVYSKPNNIYRFIWFNRNIKLLNKNLQHLYTKQQEYINLTKNAIVYFNNSYDCLVFYRQAFCFLNQFINNFLIEKYDSLFYLNVLNRISLLFKEIENCIQAKDVEKQIAFLNKLHNILSQTIITANRQYMFDIKLSYLTHHFKVLIQKVKQMELMKNKVINSQDLLKLHELINNITIKLKDCTNFLNNLNLMLCERNMDEVQEAISWLFQAVSSKEKSINLVVENIDDFRNQIQQYEKKNELLKTTIKAIELVFQNETDVHDLIIELNENCDLITKNINNLNHQSLAQNQINYEKLFYLMSQTNQALEKLKNSLTDLLNLAVNKFDDYRFFVYTLDDFRFKFFQIENLISDQDLKVPAKTLQVINKSKLELDNIFTEANNNYLGTFNLFSEKIDLLQIQLTEVICDVVSLVNLRLMCKQAFLFANKYRQKSSHIDRSLEQLTKLYNRQNYPDTLEGLINLLTKIKTNTKQDYLELN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should be optimized for stability and solubility when expressed in septin ring, membrane where it will be involved in septin ring assembly.2. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "NGIDINLIVVGESSLGKTTFVNSFLQSNDTNFRPKKTMDFVEHKATLSDGDQKFNLTIVDTPGFGDKSDNSNCWRPIATNLLHRLNAYFQNEVKMDRETSEIDSRIHGCLFFINPNGHRLQPLEIYIMKKIDQFVNIIPVIGKADTMTSDELNHFKKRVIADMVREKIRYFREPHNEKKAKIPIPFAIVGAGAPIEHDGKCIRGRAYPWGLVDIDDPKQSDFCQLRNFLLYTHIEGLKHKTHKLIYDTFRTEKLVALNAL",
    "response#2": "RGFQFNIMVVGHSGLGKSTLINSLFASHLIDSSTGKDITKEPITKTTEIKVSYHSLVEDKVRLNVNCIDTPGFGDQINNDKVWEPIVKYIKEQHSQYLRKELTAQREKHIVDTRVHAVLYFIQPNGKGLTQLDIAALKRLTDITNVIPVIAKADTLTMDERAKFREIIQHEFKKHNFRIYPYDSDDLTPEELELNDSIRSIIPFAVVGSEKEITVNGEVVRGRKTRWGAINLEDINQCEFVYLREFLIRTHLQDLIETTALIHYESFRSKQLIALKENK",
    "response#3": "RFTIMAAGPRGSGKSSFFNSLIGKEIVTSRGHEGIDLYMLNLDCEGIMQRITLIDTPGFGEGFDDSEIQETICNFIKAQLDMFIAEESKIRRNPKYEDTRVHCLLYFIPSTSSSLKSRDIAFLRKVSGLVNIIPVISKSDGLSITERIEVKRQVMEQIKHYNISIFDLDDPEVYSSPAAGNDLNSLVPFLVVSADRENFESRARNYQWGDVSIDNPDHCDLPALRELLLSTHIYGLIDYTASEIYENYRAAVLES"
  },
  {
    "reference": "MTDANLAIIILAAGQGTRMKSSTPKILHTIAGVPLVGHVLATANALSPAHVVAVVRHDRDRVAEAMSEMLPGLLIVDQDDVPGTGRAVELGLTALPSDFAGDVLIVSGDVPLLDATTIASLIAEHRAGDAAATLLSALLDDATGYGRVVRNAEGILDRIVEQKDASDDELTIGEINSGTYIFTVGPLREQLALVGTSNAQNEKYLTDVVGLLRAAGSTVAALPVSQPWLVAGVNDRVQLADAALRLNQLIVRGHQVNGVTIHDPASTWIDLDVSIAEDVELLPGVQLKGATSIERDAVIGPDTTLVDCEIKQGATIRRSEATLAVIGAWASVGPFAYLRPGSDLGAAGKIGAFVETKNASIGAGSKVPHLSYIGDTTVGVESNIGAGTITANYDGVNKHKTVVGSHVRTGSHNVFVAPVRIGDGAYTGGGTVVRKDVPAGSLAINVAPQRNMEGWVEANRAGTAAADAAAEATKNTN",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc); The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.2. The protein design should consider the endogenous proteins and enzymes present in the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway to avoid interference with native metabolic processes.3. A protein that can perform glucosamine-1-phosphate N-acetyltransferase activity, magnesium ion binding in cytoplasm to support cell wall organization, regulation of cell shape, cell morphogenesis, UDP-N-acetylglucosamine biosynthetic process, lipid A biosynthetic process. The designed protein sequence is ",
    "response#1": "VVMAAGRGTRMKSRIPKVLQRLAGRPLLHHVLDQAAHLAPRRVVLVAGHGAMEVEASCQAFTPAEGLKDLKSVRQEPQLGTGHAVQQALPVLAGDGTVLVLSGDVPLTRAETLAALVAAAQPERLALLVLAAGAGTRMKSDTPKVLHTLAGRSMLSHSLHAVAKVAPQHLVVVVGRDRERVAPAALEIGQELGRPVEIAVQEQQQGTGHAAECGLSALPDDFTGVVVVTAGDVPLLDADTLADLIATHSAEAAVATVLTTTLADPTGYGRVLRTQDREVIGIVEQADASPSQQAISEVNAGVYAFDIAALRSALSRLRSDNAQHELYLTDVISIVRSDGPDGHGRVMADADGLLERIVEERDATPEQRQIGLANSGIMAVRGRELLELLGALGNDNAKGEYYLTDAVALARARGHRVAAVRADDAEVLGVNTRAQLAEAEARLQAARRAALMEQGVTIVDPTSTWIDVDVKLAADVTLLPGVQLLGATVVAEDAVIGPDSTLKDVEVGAGARVVRTHGELAVIGAGANVGPFSYLRPGTELGAGGKIGAFVETKNAQIGDGAKVPHLSYVGDATIGEGANIGAGTIFANYDGVEKHRTVIGAHAKTASNNTFVAPVTVGDGASTGAGSVIRRDVPPGSLAVTSGPQRNVEDWVRKRRPGSAAAAAAEAARKKAKGE",
    "response#2": "VVMAAGRGTRMKSRIPKVLQRLAGRPLLHHVLDQAAHLAPRRVVLVAGHGAMEVEASCQAFTPAEGLKDLKSVRQEPQLGTGHAVQQALPVLAGDGTVLVLSGDVPLTRAETLAALVAAAQPERLALLTVELDDATGYGRIVRDADGAVSGIVEHKDASSAQRAIREVNTGILAAPTRQLKQWLARLSNDNAQGEYYLTDVIAMAAAEGVPIVTAQPANAEQVAGVNSPSQLAALERAHQLRQAEALMEAGVTVVDPATTWIDASVTFEPDAVVHPGTQLHGATRVAAGAEVGPNSTLTDTAVGPGARVVNTVAVGAEIGPEASVGPYAYLRPGTRLARKAKAGTYVEMKNAEIGEGTKVPHLSYVGDASIGEYTNIGAASVFVNYDGESKHRTTIGSHCKTGSDNMFVAPVTVGDGAYTAAGSVITKDVPPGSLAVARGQQRNIANWKRPVKRKK",
    "response#3": "VVMAAGRGTRMKSRIPKVLQRLAGRPLLHHVLDQAAHLAPRRVVLVAGHGAMEVEASCQAFTPAEGLKDLKSVRQEPQLGTGHAVQQALPVLAGDGTVLVLSGDVPLTRAETLAALVAAAQPERLALLVLAAGAGTRMKSDTPKVLHTLAGRSMLSHSLHAVAKVAPQHLVVVVGRDRERVAPAALEIGQELGRPVEIAVQEQQQGTGHAAECGLSALPDDFTGVVVVTAGDVPLLDADTLADLIATHSAEAAVATVLTTTLADPTGYGRVLRTQDREVIGIVEQADASPSQQAISEVNAGVYAFDIAALRSALSRLRSDNAQHELYLTDVISIVRSDGDVDAYVEERDVTPAQRAVREINSGVYAFDAAVLREGLSRLSTDNAQGEEYLTDVLAIARADGGRVRALLATDAWVVEGVNDRAQLAQLGAELNRRIVERHMRAGVTLADPARIDVRGTLTCGRDVEIDVGCIFEGAVSLADDVRVGAYSIIRNASIAQGTQIAAYSHIDSSSVGANCHIGPYARLRPGSQIHDGAHIGNFVEIKNSAIGQGSKANHLSYIGDATVGSRVNIGAGTITCNYDGANKFRTVIEDDAFIGSDTQLVAPVRVGKGATIGAGSTITQNAPDGVLTLSRSKQRNIEGWVLRRRPGTPAADAAERALARQGGQ"
  },
  {
    "reference": "MKRSLSGIQPSGILHIGNYFGAMKQFVDLQDSYDGFYFIADYHSLTSLTKAETLKENTYNIVLDYLAVGLDPSKSTIFLQSNVPEHTELTWLLSNITPVGLLERGHSYKDKIAKGIPSNTGLLTYPVLMAADILIYDSDVVPVGKDQKQHLEMTRDIAMKFNQQYGVEFFKLPEPLILDDSAIVPGTDGQKMSKSYNNTINMFATKKKLKEQVMSIVTDSTPLEEPKNPDNNIAKIYALFNNIDKQNELKDKFLAGNFGYGHAKTELLNSILEYFGTAREKREELEKNMDYVKDVLNEGSKKARTIAIEKIKKAKEIVGLVGNIY",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Catalyzes the attachment of tryptophan to tRNA(Trp)..2. The protein design should include a 'HIGH' region motif to ensure proper function.3. The ideal protein would be one that plays a critical role in tryptophanyl-tRNA aminoacylation through tryptophan-tRNA ligase activity within cytosol. The designed protein sequence is ",
    "response#1": "MDQNTD",
    "response#2": "MGKVVG",
    "response#3": "MERI"
  },
  {
    "reference": "MPSIHVSKCADPAIKLAHRIAEVVRSKPNCVLGLATGSTPIPVYQELARLHREEGLDFSQVRTFNLDEYAGLPPTHDQTYRFFMEEHLFSKVNIKPENVHFLNGMASDYEKECERYEQELKAIGPCDVWLLGIGHNGHIAFNEPGSPRDSRTRVVCLTQSTIDANARFFGNDKSKVPTKALSVGIATIMESREILLLATGESKREAVTKSVKGKCETHCPASFLHEHPHCRFYVDMDAGKVVDNTCCQATNQASCCGSTSCHAAKA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design must be focused on enhancing the efficiency of carbohydrate metabolic process.2. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor; for ring-opening step to enable effective catalysis of alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-   phosphate + NH4(+). The designed protein sequence is ",
    "response#1": "EMSKKAAEIVKKQIKEKPNTVLGLATGSTPLGMYKHLIEMYKRGEIDFSNVITFNLDEYIGLSPDHPQSYHYFMFHNFFNHINIKKENVHIPNGIAEDLEEECRKYEEEIEKAGGIDLQILGIGINGHIGFNEPDESIETKTHVVTLTEKTINANKRFFKSAEEVPRKAITMGLGSIMKAKKIVLLASGKNKAEAIKETIKGQLTTKVPATVLALHHR",
    "response#2": "EMSKKAAEIVKKQIKEKPNTVLGLATGSTPLGMYKHLIEMYKRGEIDFSNVITFNLDEYIGLSPDHPQSYHYFMFHNFFNHINIKKENVHIPNGIAEDLEEECRKYEEEIEKAGGIDLQILGIGINGHIGFNEPDESIETKTHVVTLTEKTINANKRFFKSAEEVPRKAITMGLGSIMKAKKIVLLASGKNKAEAIKETIKGQLTTKVPATVLALHHR",
    "response#3": "EMSKKAAEIVKKQIKEKPNTVLGLATGSTPLGMYKHLIEMYKRGEIDFSNVITFNLDEYIGLSPDHPQSYHYFMFHNFFNHINIKKENVHIPNGIAEDLEEECRKYEEEIEKAGGIDLQILGIGINGHIGFNEPDESIETKTHVVTLTEKTINANKRFFKSAEEVPRKAITMGLGSIMKAKKIVLLASGKNKAEAIKETIKGQLTTKVPATVLALHHR"
  },
  {
    "reference": "MTTRKRTDFWDIPSDEEDNDVGYDSEAAQESKGRSSVSKSTTAPSRLRRPSKRRKLSITEHGGGSDNGSGSESEDEGIDTESDEPEQEEQIEEGEQEDEEAESPIEPTSKSTPAATATTTTSSTDIDTTTSTASNPKLKPKKNKTGVIYFSSLPPYLKPFALKSLLIARGFGPITKIFLTPSVQSSSAGKRSNKRRMYSDGWVEFASKRTAKICAETLNATIVGGKKGGWYHDDVWNMKYLRGFKWADLMEQVQRERKETEARRRVEDAKARKEEKVFLAGVEKGKVFEGIRKKREEKEKKLRAGGEGDMGDGGKVSLDKPRRVFRQNEVRDREKDGVRKERKVDEDVQRVLAKIF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein's functionality will benefit from Polar residues, Acidic residues compositional bias in its structure.2. The protein must exhibit the following characteristics: Involved in the small subunit (SSU) processome assembly and function, and in the 18S rRNA synthesis; Required for the early cleavages at sites A0, A1 and A2 ..3. The designed protein should be expressed specifically in the nucleolus4. The designed protein should contain one or more RRM domains that are essential for its function. The designed protein sequence is ",
    "response#1": "PPPVIVAVMGPPGTGKSTLIKSLVRRYSKYTISQITGPITVVAGKKRRITFLECPNDLSSMIDVAKIADLVLLLIDANFGFEMETMEFLNILAPHGMPRIMGVLTHLDLFKKTSTLREAKKRLKHRFWTELYQGAKLFYLSGVLNGRYPDREILNLSRFISVMKFD",
    "response#2": "PPPVIVAVMGPPGTGKSTLIKSLVRRYSKYTISQITGPITVVAGKKRRITFLECPNDLSSMIDVAKIADLVLLLIDANFGFEMETMEFLNILAPHGMPRIMGVLTHLDLFKKTSTLREAKKRLKHRFWTELYQGAKLFYLSGVLNGRYPDREILNLSRFISVMKFCS",
    "response#3": "PPPVIVAVMGPPGTGKSTLIKSLVRRYSKYTISQITGPITVVAGKKRRITFLECPNDLSSMIDVAKIADLVLLLIDANFGFEMETMEFLNILAPHGMPRIMGVLTHLDLFKKTSTLREAKKRLKHRFWTELYQGAKLFYLSGVLNGRYPDREILNLSRFISVMKFCI"
  },
  {
    "reference": "MASQFHQLRILVWKNWLGVKRQPLWSLVLILWPVIIFIILAITRTKFPPIAKPTCYLAPRNLPSTGFFPFLQTLLCDTDTKCKDTPYGPQDLLRRKGIDITFFKESEIQRKSSSLRKDSNSSPRSAKFPERRNTSLVKVFSSSKPDAESTVADTVLAFNSSQVLERILDLEKTLKQNSTSESLRREICESHPSYTEDKTFSWNTLGKNIFNHFCISNKTLLESSIQELRNQLSQISNDPNKQMIVFQESAKVLSFFSQLQDQTALWQFLSSVPNLFQDDTKLKELLDILHKANSALLVVQKVSPRVTNNEGFRTIQRSVKHLLFTLDSSDQDGMGNTTSHWKEEDEEKLSPSSLAAQLLVLENFEDALLNISAESPYLPYLICVRNVTDNLANGSPENLKLLQSTIQLKKSFHRNGSYEDYLPSVSEIIRSKLLQLRNVTQLLCESETSSWIEETCQFPNISFGKLCEGSRFHMQLLEAAELGTEIAAGLLNHDNIISQKLTDLLTGDPNKINLNMDQFLEQALQMNYLENITQLIPVIESLLHVNNSATASEKPGQLIDMFKNIDQLKIELQRTTGISNASIDTLLAMPIPENRTEIISHIFWLYSCDSNVTNVKLEAEMMEFCNLSVPERSRLSYLTGITLLHYLDIYNFTYKVLFPREDQEPVERMIELFIKLKGFLNQMVSGARPLLDKMRSLKKIHLSRSVPLSQAIYRNRHINVPEGSFSTISQALCSQGITTDYLIAMLPSSQKSKVGNHTKDPLTYNLSK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The presence of ABC transporter domains is critical for the interaction of the designed protein with its intended targets.2. The ideal protein would be one that plays a critical role in intracellular protein transport, keratinization through ATPase-coupled transmembrane transporter activity within plasma membrane. The designed protein sequence is ",
    "response#1": "TTGVIMENVTAFWEEGFGELLEKVQLNGADRKLSNGDNNHSLGHLCFLGEPVLKNINFNIEKGEMLAITGSTGAGKTSLLMMILGELEASEGKIRHSGRISFCSQFSWIMPGTIKENIIFGVSYDEYRYKSIVKACQLQEDISKFAGKDHTVLGEGGVTLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEEQIFENCVCKLMANKTRILVTSKMEHLKKADKILILHEGSTYFYGTFSELQSLRPDFSSKLMGYDTFDQFTEDRRSSILTETLRRFL",
    "response#2": "PGELLAVVGPVGAGKSSLLSALLGELPRSQGLVSVHGRIAYVSQQPWVFAGTVRSNILFGKKYEKERYEKVIKACALRKDLQLLEDGDLTVIGDRGATLSGGQKARVNLARAVYQDADVYLLDDPLSAVDAEVGRHLFELCICQTLHEKITVLVTHQLQYLKAL",
    "response#3": "IFYGILLYLGEVTKAVQPLLLGRIIASYDPDNQMERSIAIYLGIGLCLLFIVRTLLLHPAIFGLHRIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNNLNKFDEGLALAHFVWIAPLQVALLMGLLWDLLQFSAFCGLGLLIILVVLQAILGKMMVKYRDQRAAKINERLVITSEIIDNIHSVKAYCWESAMEKIIKNLREVELKLTRKAAYMRFLTSSAFFFSGFFVVFLSVLPYTVINGILSPKRSIKPILFFLLIVAMTLLSVRISLVNSEWYKNMYTSLQEFNEHVFWQQMGLFCVIAASSVSAALVSYYLEQRFVINWIEWLNEQLVNKWMAHRAYYKTQYLSENLDNPDQRIQQDVQSYVKTTLSLSTGVIDAVTSMISYTILLWGLAGPMIVLGVEIPHMMVFLVFGYVIFTTLIAFWLGRPLISLNFINERLNANYRYSLIRIKEYAESIAFYAGEKVEKNQLYQQFNAVIHNMWVIIFRTLKFSGFNLVVSQISVVFPLLIQVGRYFRNVSFSYQLNTPFSTAALKDVSFDIPDGSFTSIVGHTGSGKSTVLQLIDGLLIPEKGHIQAGSFQMNSGSNAKHLKKFRKNVGFIFQFSENQLFEETVEKDLIFGPKNFGVEEKIAKEKARKVLKIVNLPESFLQKSPLDLSGGQRRRVAIASILISDPQLLLLDEPVIGLDPGSKDEIMNLFSRLNQQGKTIVMVSHEMDDVADYSDQIIVLNAGK"
  },
  {
    "reference": "MENNNNNFIEEEEELSEPKEKCGVFAIYAPELDVSRIAFFGLVALQHRGQESCGIATYDEFQSVHVETGMGLVNQVFNETNLKPLKGKMAIGHTRYSTAGKSTLVNAQPVIVQTLHGQIGIVQNGNLTTAKSLRKELMQKGVGFFIDSDVEVITQLLSNNPEGCDPHKPNWENRIAHFMSKAEAAYALCLMTPNGIYGVRDSLGMRPLCLGSLEVPCKDDPTKTITRYVLTSESCAIGTIGAKFIRDVRPGEIVHINENGITSFIGRSPSDNPALCVFEYVYFSRPDSSMEGQLIHIVRQRMGETLARESPPPQTCSNNDTIVIGVPDSSLPAAIGYAKQSGIPFTEGLTKNRYIHRTFIQPSDHLRQQGIKLKFNPLTENIQGKKVILVDDSIVRANTIKALIKLIRGAGATEIHVRISSPPVLHPCHMGIDMATYDQLIGHNRTVEEIREYIGAESLQYLTLEGLMKSVNIGIKPQAETNSTPCFSTSSPTTTKIKINENNQKHCVACFTGDYPCSLDF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must be engineered to have a cooperative binding mechanism with [4Fe-4S] cluster to ensure proper regulation.2. The designed protein should have a binding site that is specific to the target [4Fe-4S] cluster, Mg(2+) ligand.3. A protein that can modulate the overall flux of metabolites through the Purine metabolism pathway, enhancing or suppressing its activity as needed.4. The designed protein must have a Nucleophile active site that is highly conserved among related enzymes.5. A protein that contributes to 'de novo' IMP biosynthetic process by functioning in cytoplasm, proteasome core complex with amidophosphoribosyltransferase activity. The designed protein sequence is ",
    "response#1": "TTACISSQVGCSLACEFCATGKLKLMKNLEAYEIYDQVAEIYKDAEILQSKPLQNIVYMGMGEPLLNYKNMLTSTQRISDDKGLGMSPKRITVSTAGIAKMITKLADDNVRFNLALSLHAANDEKRNKIMGINETNSLEVLAEALKYFYRKTATRVTYEYIIFKDFNDTIDDARELLAFCKHVPCKVNIIEYNPIDGGIFEQADPEKVDAFAEFLE",
    "response#2": "VELARESPAEGDVVIAVPDSGRTAALGFAHESGIPYMEGLIKNRYIGRTFIMPSGRGLKVKLKLSPVREVVNGKRVVLVDDSIVRGTTMTRIVKMLRDAGAREVHVRIASPPIRYPCYMGIDIHGDEVKFGGGKVI",
    "response#3": "GRERRTVCVSTQVGCALGCPYCATGQQGFERNLSAAEIIGQVLYFARTFGTMTLEQEVGNNRNRVTHVVFMGMGEPLANYDNVVRAIAILNSQKGMGLGFHQVTLSTAGLVPQIARLADEKFQFQLAVSLHAAADELRISLVPVNQKYPLDQLIPACREYAVRSGRKVFIEYALFEGVNDSVGQADALIKLLEGLSCSINLIVGNPTCSDGFQPSSREKALAFQKKLIAGGIRTMLRVSRGSDIG"
  },
  {
    "reference": "MPGRLGLLWCWGLLWSCCCCSPPPGLPLLPAGGTLLPDSRSPGQGSSREQPPPQPASSSSSSGFLYRRLKSHEKREMQKEILSVLGLPHRPRPLHWLQQQQAAPPPQQPQPGRLNSAPLFMLDLYNALSSEEEGEGGQRTAPRSQPPTSHTLQHKTLIPQQGPSGSTSSPLASSQDSAFLNDADMVMSFVNLVEYDKEFTPHQRHHKEFKFNLSQIPEGEAVTAAEFRIYKDCVMGSFKNQTFLISIYQVLQEHQNRDSDLFLLDTRAVWASEEGWLEFDITATSNMWVMNPQHNMGLQLSVVTRDGLNVNPREAGLIGRDGPYDKQPFMVAFFKVSEIHVRTTRSVAGRRRQQSRNRSTQSQDVPRVSSITDYNSSDLKTACRKHELYVSFQDLGWQDWIIAPKGYAANYCDGECSFPLNAHMNATNHAIVQTLVHLMNPEYVPKPCCAPTKLNAISVLYFDDNSNVILKKYRNMVVRACGCH",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should contain one or more TGF-beta family profile domains that are essential for its function.2. The designed protein should be involved in positive regulation of pathway-restricted SMAD protein phosphorylation, positive regulation of osteoblast differentiation, eye development, positive regulation of endothelial cell differentiation, type B pancreatic cell development by localizing to extracellular region and exhibiting growth factor activity, protein heterodimerization activity, BMP receptor binding.3. Incorporate a signal peptide in the protein design.4. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "CRRHPLYVDFSDVGWNDWIVAPPGYHAFYCQGDCPFPLTDHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCS",
    "response#2": "ERTDTNFLNEFELRLLNMFGLKRKPTPSKSAVVPQYMLDLYYMHSENDDPNIRRPRSTMGKHVERAASRANTIRSFHHEEAFEALSSLKGKTTQQFFFNLTSIPGEELISAAELRIFRDQVLGDASTSGFHRINIYEVFRPALAPSKEPLTRLLDTRLVQDSHTRWESFDVGSAVARWARESQHNHGLLVEVLHPKESEVSEEAESNRRKHVRVSRSLHADEDSWAQARPLLVTA",
    "response#3": "RVTNVAENSSSDQRQACKKHELYVSFRDLGWQDWIIAPEGYAAYYCEGECAFPLNSYMNATNHAIVQTC"
  },
  {
    "reference": "MEDYIVRATAKEGTIRALAAITTNMVKEAQKVHGLSPLATVALGRTMTAAAMMSTTLKEENAVITLQIKGDGPIGGIVVVVDSSANVKGYVHNPLVYLPLNSQGKYDVAGAVGNGYLNVIKDLGLREPYVGHVDLVSGEIAEDITYYYAYSEQVPTATALGVLTNATEIVVSAGGFILQLMPGADDDTISFIENKISSIPPVSTLLAQNKSPEDILEMLLSEKDMKIIGKSPCRYLCNCSRERMERNIMTLGKEEIMGMINENHGAEAHCHFCNKKYWFSEEDLLRLVKIIESQKS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: Protects both thermally unfolding and oxidatively damaged proteins from irreversible aggregation. The designed protein sequence is ",
    "response#1": "MPLLDSQGRVINTWADIINR",
    "response#2": "LQV",
    "response#3": "MVSVT"
  },
  {
    "reference": "MADKNQEIGGIHFPFPFTPYSIQKDFMTVLYQVLEDGKIGIFESPTGTGKSLSLICGALSWLRDFEEKKRQEEAHLLKTGTAPSNDGKDQPPCISSSCQETPDTLGPTGEPDWVTQFVQKKEERDLVDRLKKEQIRRKKREERLQQLRHNTQLKYAAKRERQEEEETERLLRLSRDILAAGTEAEPLEQLASGEEELVLSEYESDEEKGAPNGLDEDEDDLEEEHVTKIYYCSRTHSQLAQFVHEVQKSPFGKDTRLVSLGSRQNLCINQDVKNLGSVQLINDRCMELQRSRHESKSRAEDQEPKRRRQAPQAACPFYSHRHLQLLQDQVLLEVKDIEQLVALGKEAGACPYYGSRFAIPAAQVVVLPYQMLLHTATRQAAGIRLQGQVVIIDEAHNLIDTITGIHSTEVSGSQLCQAHSQLLQYMERYRKRLKAKNLMYIKQILYLLEKFVTVLGGNIKQNPNTQSLSQTGTELKTINDFLFQSQIDNINLFKVRRYCEKSMVSRKLFGFTERYGAVLAPPREQPRLTGFQHFLQSLQPGVIEAPTAPMEEGEARVPRPASPLMHIEGFLEALTTANQDGRVMLSRQGSLSQSSLKFLLLNPAVPFAQVVKECRAVVIAGGTMQPVSDFREQLLTCAGVEAERLVEFSCGHVIPPDHILPLVLCSGPSNQQLEFTYLKRELPQMMDETGRILCNLCNVVPGGVICFFPSYEYQRQVHAHWDKSGLLARLAVRKKIFQEPKRANQVEQVLTEYSRCIQCHGQAGGMVN",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should be able to enhance the efficiency of cellular response to DNA damage stimulus, replication fork processing. The designed protein sequence is ",
    "response#1": "MRPTKIEDIIGQQHLVAEDKIIGRMVQAKHLSSMILYGPPGIGKTSIATAIAGSTSIAFRKLNAVINNKKDMEIVAQEAKMSGQVILILDEVHRLDKGKQDFLLPYLENGMIILIGAG",
    "response#2": "PPCFSCAFDNREGKFSGESYLASGALKRLILNLDPLPTNFEEDTVELFGFQWVTETALVYSCRELFHLFRQQIFNLESLVQVSCDFGKIATLHAKADSIRQQCVVFLHYIKVFIFRCLKVQEAESHSRPAHPYEALEAQLPSMLVDELRGLLLYIGHLAALPSVTVGAFVNQNQMKLFPPSWHLLHLYLDTHWLVLEILHILGEKLKQVVYGRQFIGQAGDNLTNVSLFEEHCEHLFCDLICLSLNRFDKVMPSEALLISHCPCSCVKELWVLLIHLLDHRRKWSVADSFWNWLNKLLRTLFEKSSDQRRSSVSLTQAKDPLGFSWWISVHVASLYQIDRHGVSDKMKQMESNWSFIEELLKRSVTVQDSILEEQLRMHLHCCLTLCDFWEPNISVVTILWEYYSKNLNSSFSISWLPLKGLTNIIKSPLSMLTLVRNCCSDKQDPDLYKSSSSYIIFLCILAKVVKKAMRTSGPHPWKQVKGRIYSKFHQKRMEELTEVGLQNFFSLFLLLAAVAEIEDVASHVLDLLRFLRPASMSSHGALVWKGQMAFLLMYAQKNLDIGVWAEKLSCEFQEKAKEFLVSKNDEMVQRHALWTLLCIYIDGVQEVFETSSCLYPSHEHLLNDGFSMLLPACRESELRTVLNFLQAVLARIRSVHQQLCQELQRENVDLTVQSSLSAKERPLAAVAGALWRHFFSFLKSQRMI",
    "response#3": "LLFAGPPGTGKTTAALALAHELYGDAWRENFLELNASDERGIDVIRHKVKEFARAKPIGDVPFKIVFLDEADALTRDAQQALRRIMEKYSQSTRFILSCNYFSKIIEPIQSRVTVFKC"
  },
  {
    "reference": "MTVNLLDYDREGLTDFLTGIGEKSFRAKQILKWVHQFGRSDFAEMSDLAKGLREKLSKLAVISVPEVMRDHIAADGTRKWLLSVGANNGIESVFIPEKNRGTLCVSSQVGCALACAFCSTGKQGFNRNLTVAEIIGQLWLADKSLRESAGSQSSLLAQHDSSIPQTLQSPRVVSNIVMMGMGEPLANFENVVTALNLMLDDHAYGLSRRRVTVSTSGLVPAMDRLRERCPVALAVSLHAPNDALRDYLVPINKKYPIKALLAACQRYLQVAPRDFITFEYVMLDGVNDSASDAKELIKLVKDVPCKFNLIPFNTFPNSEFKRSSPESVRVFRDILIQAGLVTTVRKTRGDDIAAACGQLAGQVRDKTRRAGKVNLESMR",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein with tRNA (adenine-C2-)-methyltransferase activity, metal ion binding, rRNA (adenine-C2-)-methyltransferase activity is needed to enhance rRNA base methylation in a specific context.2. The presence of [4Fe-4S] cluster is necessary for the protein to function in the desired environment.3. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs; m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity..4. The design of the protein must focus on the precise arrangement of amino acids in the S-methylcysteine intermediate, Proton acceptor to enable effective catalysis of adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.5. The [4Fe-4S] cluster binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MNIYGMTRYDLEQYFINNNMHKFKALQVFEWLYIHKVKSFFEMTNIKKELQEQLESHFATNLLDFDLEGLAAFCERLGEKRFRATQLFRWIHQRGASDFEQMTDLAKSLRGKLAGVAQVTALPVIAQHISADGTIKWLFDVGDGNAVEAVFIPEDDRGTLCVSSQAGCAVGCRFCSTGHQGFSRNLSTAEIVAQLWFAEHFLRKHLARDERIISNVVMMGMGEPLQNYAALVPALRTMLDDNAYGLSRRRVTVSTSGVVPMIDRLAQDCPVAMAVSLHAPNDLLRDDLVPLNRKYPIAELLAACKRYLEHAPRDFITFEYCMLDGVNDSDAHAQQLIDLVRSQDVSCKFNLIPFNPFPASGLLRSPAQRVQAFARKLADAGIITTVRKTRGDDIDAACGQLAGDVKDRTNAVKILPYPHSIKKGSRVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGSGEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLHSPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALAKLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKVKYKEI",
    "response#2": "MNIYGMTRYDLEQYFINNNMHKFKALQVFEWLYIHKVKSFFEMTNIKKELQEQLEQAFAPGRATVCISTQVGCQVRCVFCSSGSEGLIRNLTAAEITDQVLLSCRHLGAKINNIVVMGMGEPLHNLDNLTRALDHLNAPDKFALGARHITISTSGIVPGIRQLADLHRPWNLAISLHATSDQQRAQIIPPACRYPIGEIISACQYHRQQTNRMPTLEYALLKDFNDSPEDAVRLAALAARLYAKINLIPCNPGPSRYQAPAPAKCRAFLERLIRAGAQATLEVKDASGAVLGRYPERVTMCISSQAGCGMNCPFCATGQAGLTRNLSTAEIVEQVLAGSRSLDRGQIAGGPGRVSNVVFMGMGEPMANYGAVVESVRRLISPAPSGLGLSARGITVSTVGLVPRMKDLATEGLPVTLALSLHAPDDELRNTLVPVNTRWPVAEVLNAAWEYADRTHRRISIEYALIRDINDQAWRAELLADLLVGHLVHVNLIPLNPTPGSIWTASRPADEETFVRILRDRGLAVTVRGTRGREIDGACGQLRRSQR",
    "response#3": "MNIYGMTRYDLEQYFINNNMHKFKALQVFEWLYIHKVKSFFEMTNIKKELQEQLEKEYFLSKIKSDTRKTICVSSQVGCPVRCSFCASGKKGFFRNLKAFEIVEQVLLIAKDINEKSTNIVFMGMGEPLLNFDEVVKAVKIMSDDKYLNISQRRITISTAGILENIKRLKDENLKVYLALSLHGATDEIRNRIIPYSKKYKLQDILKELRLYFEKTKRDILFEYILIESVNDSIEDAKKLVHLLKNFQCTINLIPYNEVENLSYKRPSGVKILKFKNYLISKGLNVVQRYTKGKEISAACGQLRSEKLKR"
  },
  {
    "reference": "MTSVIFMGTPEFSVPVLEGLIEAGYEIRAVVTQPDKKVGRKQKIAKTPAKIAAEKHDLPVLQPVKLSGSEEMNQLIDMHADLIVTAAYGQFLPTKFLKSVNIAAVNVHGSLLPKYRGGAPIQYSLINGDKETGITIMEMVKKMDAGDIYAQEAIKIEPEDNAGTLFSKLSILGRDLLLKTLPSIIDGSVKKTPQDPDKVVFSPNITKEQERLSIDMTAEQANNMIRALNPDPGAYLMINGQRFKVWEAEVASDSSSLEAGTVVANKGRFAISFADNTVLNLLEVQPSGKKRMNIKNFLNGQGSKFVTGEEIVDK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must have high specificity towards methionyl-tRNA formyltransferase activity.2. The protein should be designed to have high stability and activity towards (6S)-10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) =   (6S)-5,6,7,8-tetrahydrofolate + H(+) + N-formyl-L-methionyl-   tRNA(fMet). The designed protein sequence is ",
    "response#1": "IKKEDLFVDFSKGAETVKNKIRAYDSRPGARAFLNGKEVKLFGAMAVDSSNDEPGLIHYIDREGAWIGTGKGMVKVKYLQLPGKKKLTFWELRNGW",
    "response#2": "FMGTPEFSVPTLRLLVDAGHRIVAVYTQPPRPGGRRGLDLQKSPVHQAAELLGLPVFTPVNFKDPEERERFAALKADVAVVVAYGLLLPEAVLNGTRDGCYNGHASLLPRWRGAAPIQRAIMAGDAETGMMVMKMDKGLDTGAVALTRKVEIGPNMTAGELHDRLMQVGAKAMAETGARL",
    "response#3": "MKLVFAGTPEVAVPALDALIASDRHEVAAVVTRPDAPAGRGRRLVASPVAERAQEAGIEVLKPAKPRDEEFLARLREIAPDCCPVVAYGALLPKVALDVPARGWVNLHFSLLPAWRGAAPVQHAVMAGDEVTGASTFLIEEGLDSGPVYGVLTEEVRPTDTSGDLLTRLAFAGAGLA"
  },
  {
    "reference": "MLKDAHQKLIKHRQSTKLSMTDFACQAAPVKQPLPLELPLPLSVQLQLLAKTPTSDHAPMHSTPPTTPPTPPPLPLNMSQSASAVTEAATPENSLPATPPSEGALAVPSAPQDHYSLRWNNHQNHILRAFDALLKTKTLVDVTLVCAETSIRAHKMVLSACSPFFQRVFAETPCKHPVIVLKDFRGWVVQAIVDFMYRGEISVPQQRLQTLIQAGESLQVRGLVESSVPEHTPTPAASPDDFGMLDTSMLSSTFEDECPTMVRPSKGGKLLMPSARLFGNASSAIAALGLRRKREQESDRDLESDQELGGSSPMPRRKQARPRRRSGDVPHDFTLNKTDAESLQTVIKHELLERAERDQEEAPDQDNSQGEAEKISSSPAKTLVERAKEQKSMKEEGSDQPRSLNENHHQLELDDEDDDDQDHEEEEEQDIEELIHTTNELRRQAAAAAANAAAMSPNPSPCLSDGPEDLCTTKKGKELISGPSSSADCESNNNNSSKLQDNNQRIMLSLKDIRQLNANPNPTAIHTPTSCSGGNNGLLTFPPPGLRPPGLPDSPPCHMEALEAQMHAAAAAAVAAAGSGEHPFHHMEHQMEMSLAAAAAAAAMHQREPRDPRDGRDHNAFASNLLGPMGMPPFGGHNGGHPGNSGPGNGCPGQAAHERLEESMNRLSKELGKEFGKEFGKEFGKEFGPASPMSLQGPFNAPDGPPHPPSPLPFPGMSSAMTLTPPHMFGLDSPLGLFPPGIDPGKLYNPLMEMSDPRDMPGGPPPFL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should emphasize Pro residues, Polar residues, Basic and acidic residues compositional bias.2. The protein must exhibit the following characteristics: Has a regulatory role during midline cell development..3. The presence of HTH psq-type, BTB domains is critical for the interaction of the designed protein with its intended targets.4. The ideal protein would be one that plays a critical role in male courtship behavior, negative gravitaxis, gravitaxis, larval feeding behavior, larval walking behavior through DNA binding within nucleus. The designed protein sequence is ",
    "response#1": "PEEDVATRVIRAFNQQHSSYTTQHGVCNVDPDCIPHLSRAGGWELFARELNPLIQAIIEFAKSIDGFMNLPQETQIQLLKGSVFELSLVFAAMYYNVDAQAVCGERYSVPFACLIAEDDAEMQLIVEVNNTLQEIVHLQPHQSELALLAAGLILEQVSSSHGIGILDTATIATAETLKNALYQSVMPRIGCMEDTIHRIQDVETRIRQTARLHQEALQNFRMSDPTSSEKLPALYKELFTAR",
    "response#2": "LHAERCEAKRLERLKGRQFYHSQTMQPITFEQVMSNEDSENETDDYALDISERLRLERLVGVSKEEKRYMYLWNIFVRKQRVIADGHVPWACEEFAKLHKEEMKNSSSFDWWWRMFRIKLWNNGLICAKTFHKCTS",
    "response#3": "PGSILVNTNLRALINKHTFASFPQHFQQYLLLLLPEVDRQMGSDGILRLSTSALNNEFFAYAAQGWKQRLAEGEFTPEMQLRIRQEIEKEKKTEPWKEKFFERFYGERSI"
  },
  {
    "reference": "MQLIDLISGDKIKVTLTADISELDITGLTADSRDVRPGFLFAALPGLTVDGRNYISTAIEKGAAAVLAPDDTDPPDGAALIKAADARATLAHMAAAFYRGQPGTVAAVTGTNGKTSVAHFTSQIWSKIGNCAVSIGTLGLQSHDGTIMRNLTTPEPVSLHENLAKLDRDGVTHAVLEASSHGLDQHRIDGIRLTAAAFTNLTRDHLDYHGTAENYFTAKARLFAELLPSDGVAVLNADSPDSVLLSKLFGGRTLTYGRAGSDFILRDAAHTPRGTYVKLTIMGRNINVELPLAGTFQIHNVLCAVGLTIGCGADCDTVINCLDTLESVPGRMQLAGTVSGGSVYIDYAHTPDALLNVLTALRPHTKGRLISVVGCGGDRDRGKRPEMGSISGRLADITIVTDDNPRSEDASQIRSAILAAAPGAMEIGNRSDAIHAAISEITSGDVVLIAGKGHETGQIVGDKTLPFNDLDIAQTAIDTAGGKLS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The ATP binding site should be stable and able to withstand changes in environmental conditions.2. We need a protein that can perform ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate with high efficiency.3. The ideal protein would be one that plays a critical role in cell cycle, cell division, regulation of cell shape, cell wall organization through magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity within cytoplasm.4. The presence of Mur ligase N-terminal catalytic domains is critical for the interaction of the designed protein with its intended targets.5. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.6. The designed protein should contain at least one Meso-diaminopimelate recognition motif motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "MKLKDLISLLKINSVVGNVEYDITGVCYDSRKVEPGNLFVCVVGEKTDGHLFAQQAVQSGAVAVMGERIPEVSSLVIDSRKIEKNSLFVALRGTQVDGHAYIDQAIESGAGAVLCEKLPADLREGVTYVEVLDTNQALAIAAFYDYPTKNLKVIGITGTNGKTSTSQIIANLLDACQKKTGLIGTIKNIIGNEVIPAERTTPESLDLQELLAKMVDDGVDYCVMEVSSHALELDRVYGCSFAVGVFTNLTQDHLDFHKTMENYYKAKARLFELLGNSYTAIVNGDDEYGNVLKENSSAKVITYGIKKGADISALNIKTTPNGTSFELSYKGIRHKVASALIGKFNIYNALAAIAAGVVEGVDLDTIVKGIEMVKTVPGRMEAFSSAGKPTTVVDYAHSPDALSSALDACRLHCHGKLWLVFGCGGDRDVGKRALMAKVAESKADHIIITNDNPRSENPEAIAADIIAGLGKTAHFEKILDRKTAVLMALKQADADDVILCAGKGHEKYQIIGKKKYHFDDREVVIEELGKY",
    "response#2": "MSVSGMTLDSRHVKQGDVFVAIKGHSVDGRQFMAKAIENGAAAVLTHNDDNTFSLTEQNGVAVIALPSLNTLLSAIAKRFYSNPSKKMEIIGVTGTNGKTSVVHHTRQIWNLIGKNNSAIGTLGIFYNNKKIINSELTTSDSITNHKYLSVLSKKKCENIIFEASSIGLDQHRLFPLKFDKVAFTNLSRDHLDYHKKFEIYKKSKSLLFTNYTKRNSVAVIFADSKYSHYFKDICNKRGLKILDYGKKASFLKIKKIKKINRNFHVTFELNKQNLNFEINVSSEYEILNRICSLLSILYSMGVSLNSLANALSTFKGVKGRMQRVDLGEGSPAVFVDFAHTPEAVTAAIGAVAGSARVLAVLGAGGDRDPGKREPMGAAAAAAADVVVVTDDNPRTEDPAAIRARVLAGARGLDRAGVQVVDGGDRRSAITTALGLAGPDDAVVVLGKGHEKYQIIGSSKIPFDDKEVIKKFLEVRNLTRDSRCVGVGDVFIAHKGRHYDGNDFAAHAVDNGAIAVLSDSSFKDLPIQYISVNNRRDALIRLSTRLYQKFKCKIIGTGSTGKSSTKEMLAQVLNTSSPTLKTQANENNMIGLCKTIPRIRPEHRFGVFELGTNAFGEIAALADICNPDAAIIINIGPSHLEKLIDEDGVFREKTALFNRPLEIRLFDGDDPRFSIYADKGKSVGFSDNSDFHITDLECDDTFCG",
    "response#3": "MKLKELLHNLKIEKIVGSIDLDVSIENDHRKVNSGSLFICIKGYTVDGHDLAPEAVKNGAVAIVSEKPLDVDVPVVIVKSTAKVMAQVADQFYGNPSKKMKLIGITGTNGKTSTSQIIANLLDACQKKVGLIGTIEVIIGEEKVKAANTTPESYTVQQYFAQMVDAGCEYCVMEVSSHALALDRVYGCEFDVGVFTNLTQDHLDFHKTMENYYEAKKKLFTECKIGIINIDDEYGKRVMQETDGICITYGIEKNADYRASNIVLSQKGTQFNVTITGTNGKTTTSMLLYQALKELGYSCGWIGTLGYYINDEHFITHHTTPDILELNQILARMVEKGIKYVSMEVSSHALALDRVYGIEFDYCLFTNLSREHLDFHQTMEEYGAAKKKLFEPTIQHKAVGLINIDDNFGKTLFEELKSANAYVFSIGTSDADFIIRTEHSHIPHSTTGNRFSLQTPEGIINIRSSLLGSFNMNNLAMCAAYGTALLLGEDKLSVLRALSSFKGVEGRFEPVPNDRGIKVFVDYAHTPDAMENVLQACQDIEHKRILCLFGAGGDRDKGKRPLMLQTALHYANAVFITDDNPRFEDPDAIIRDIVSGTELWLPWWIIRNRKEAIHSILRLAQPGDIVLLCGKGHETYQIFKDKTIHFDEREIVKEAIKELK"
  },
  {
    "reference": "MMFRLTSVGCILLVIAFLNLVGLTNACTSEGYSCSSDSNCCKNVCCWNVCESHCRHPGKRTRLQGFFKHRR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be optimized for toxin activity.2. Include a signal peptide as an essential component of the protein.3. For general function, the protein need meet that Probable neurotoxin. The designed protein sequence is ",
    "response#1": "QDPNFKGINRQLDRGWRGSTDITIQRGDDVFKENYVTLPGTFDECYPTYLYQKIDESKLKPYTRYQLRGYIEDSQDLEIYLIRYNAE",
    "response#2": "QDPNFKGINRQLDRGWRGSTDITIQRGDDVFKENYVTLPGTFDECYPTYLYQKIDESKLKPYTRYQLRGYIEDSQDLEIYLIRYNAD",
    "response#3": "ITQIPLVKAHTLQSGTTVVRGPGFTGGDILRRTSGGPFAYTIVNINGQLPQRYRARIRYASTTNLRIYVTVAGERIFAGQFNKTMDTGDPLTFQSFSYATINTAFTFPMSQSSFTVGADTFSSGNEVYIDRFELIPVI"
  },
  {
    "reference": "MPIQRLSKAARIILVGAPGVGKGTQTERLMNKFTELSAISSGDLLRKNVRERTPLGIQAEHTMKSGKLVPDAMILRLIVNELTTRGWIRDNALRPYALNFSSTDMPETTVDAVMIPSAVRAPRYTFSNRPSASFILDGFPRTAVQAIQLDNIVPINMVVNLNTPSDIIIDRICNRWVHEASGRVYNTTFNAPKVDGKDDITGEPLTRRADDDPEVWKARLKSFKENNEPLLEHYDKLGVLWTCTHYTLLIICSNHCHCTMAIVEESHPLHKSAEDFEAMHEGVRHHAPALDKGANAGSSTDVTDNTPSLTRPQPASPQHQFDDILDDPEYDHISDIVELRKNHLVYHRDPGAIRRHAMAADFSEGRLETFVGSPQKELAGTQNQYVSYQVTTKSDFQSFQKPEFSVRRRFTDFVFLWKQLSKEYPQCAVPPLPDKHKMEYVRGDRFGPDFTQRRAHSLHRFLKRIALHPVLRRAALVINFLESSDWNQHMKGRSSRAASGSEQGSGGFVDAIADTFVNTFTKIHKPDQRFIEVSERANKLSEDLNNVEKVTVKVARRQGDLETDYADLATQCQKLTTMEPAVEGNLTSFAASVNTTSQGFKSIRDHTDQNYLTSLRDMDAYIQAVKTLLRTREGKQLDFEQLSEYLAKSAADRDSLASATGAGMGASGFLRSKVEDFRGIDHDQARRQRVRKLEVEIERLTGEVEMSKKASEAFDEHTVKEVSDFERIKAIEFKDTLGDLADAHVEFFQGTIETWEKFLEDMRKEEDQ",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have nucleoside triphosphate adenylate kinase activity, phosphatidylinositol binding, adenylate kinase activity, ATP binding to facilitate AMP metabolic process, GTP metabolic process, ADP biosynthetic process, phosphorylation.2. The protein should have a low Km value for a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'-   diphosphate + ADP.3. For general function, the protein need meet that Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates The designed protein sequence is ",
    "response#1": "KLPILFIVGGPGSGKGTQCERIVAKYGFSHLSSGDLLREEVKSGSPRGAQLSKIMEAGELVPQEIVLDLLKEAMLKEVAKGSKGFLIDGYPREVKQGELFEKEIQQAKSVIFFDVSDDILVERLLNRAKTRQSQVSHYH",
    "response#2": "KLPILFIVGGPGSGKGTQCERIVAKYGFSHLSSGDLLREEVKSGSPRGAQLSKIMEAGELVPQEIVLDLLKEAMLKEVAKGSKGFLIDGYPREVKQGELFEKEIQQAKSVIFFDVSDDILVERLLNRAKTRQSQVSHYH",
    "response#3": "KLPILFIVGGPGSGKGTQCERIVAKYGFSHLSSGDLLREEVKSGSPRGAQLSKIMEAGELVPQEIVLDLLKEAMLKEVAKGSKGFLIDGYPREVKQGELFEKEIQQAKSVIFFDVSDDILVERLLNRAKTRQSQVSHYC"
  },
  {
    "reference": "MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTFRGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPADLVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSFEPLSGPAVRPYNRREDAKYRNQQGAVASRIDKD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should interact with enzymes and substrates within the dUMP from dCTP (dUTP route): step 1/2 pathway to enhance or regulate its activity.2. The designed protein should have dCTP deaminase activity to facilitate dUTP biosynthetic process, response to radiation, dUMP biosynthetic process.3. The Proton donor/acceptor of the protein must be engineered to enable effective catalysis of dCTP + H(+) + H2O = dUTP + NH4(+). The designed protein sequence is ",
    "response#1": "DLTSAVEVPDGEPFVLHPGEFVLASTLEVISLGDQLAGRLEGKSSLGRLGLLTHSTAGFIDPGFSGHVTLELSNVANLPIMLWPGMKIGQLCFL",
    "response#2": "GYDLRISREFKVFTNVYNSLVDPKCFTEDALISIVDDVCIIPPNSFALARSVEYFRIPRNVLTVCIGKSTYARCGLIVNVTPFEPEWEGYVTIEISNTTPLPAKVYANEGIAQVLK",
    "response#3": "GYDIRCSDEFKLFTNINSAIVDPKNFDSNSFVDVKSNICLIPPNSFALARTVEYFRIPRNVLTICLGKSTYARCGIIVNVTPFEPEWEGFVTLEFSNTTPLPAKIYANEGVAQVIL"
  },
  {
    "reference": "MLKQVEIFTDGSCLGNPGPGGYGAILRYRQHEKALSAGYRLTTNNRMELMAAIVALETLTSACQVALFSDSQYVRQGITQWIHGWKRRDWKTADKKPVKNVDLWQRLDQAIGPHQVEWIWIKGHAGHPENERCDELARRAAGTPTQDDSGYTPG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.2. The protein can be expressed in cytoplasm and perform RNA-DNA hybrid ribonuclease activity without triggering undesirable cellular responses.3. The protein must exhibit the following characteristics: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids..4. The designed protein should contain one or more RNase H type-1 domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MSVIGSDEVGTGDYFGPITVACAYADKTKLSLMKELGVKDSKDLKDPQIIEIAKLLIKTIPYSLLVLKNEKYNQMQEKGMSQGKMKALLHNQAISNLLRKMNGVKPEAILIDQFAEPGIYFKHLSGRDIVKEKTFFSTKAESIHLSVAAASIIARYSFLIEMDKLSREAGMTIPKGAGPHVDEAAAKLIINKGEGALRTFTKLHFANTQKAKRLVEA",
    "response#2": "MPELFAYTDGACSGNPGPGGWGVLLRAIEGETVLKERELCGGEAETTNNRMELLAAINALETLERPSKITVVTDSAYVKNGVTGWIFGWKRNGWKTAGKKPVKNVELWQRLDLAQARHDVTWKWVKGHAGHPENERADELARAGMIR",
    "response#3": "MPELFAYTDGACSGNPGPGGWGVLLRAIEGETVLKERELCGGEAETTNNRMELLAAINALETLERPSKITVVTDSAYVKNGVTGWIFGWKRNGWKTAGKKPVKNVELWQRLDLAQARHDVTWKWVKGHAGHPENERADELARAGMA"
  },
  {
    "reference": "MAAAATAAEGVPSRGPPGEVIHLNVGGKRFSTSRQTLTWIPDSFFSSLLSGRISTLKDETGAIFIDRDPTVFAPILNFLRTKELDPRGVHGSSLLHEAQFYGLTPLVRRLQLREELDRSSCGNVLFNGYLPPPVFPVKRRNRHSLVGPQQLGGRPAPVRRSNTMPPNLGNAGLLGRMLDEKTPPSPSGQPEEPGMVRLVCGHHNWIAVAYTQFLVCYRLKEASGWQLVFSSPRLDWPIERLALTARVHGGALGEHDKMVAAATGSEILLWALQAEGGGSEIGVFHLGVPVEALFFVGNQLIATSHTGRIGVWNAVTKHWQVQEVQPITSYDAAGSFLLLGCNNGSIYYVDVQKFPLRMKDNDLLVSELYRDPAEDGVTALSVYLTPKTSDSGNWIEIAYGTSSGGVRVIVQHPETVGSGPQLFQTFTVHRSPVTKIMLSEKHLISVCADNNHVRTWSVTRFRGMISTQPGSTPLASFKILALESADGHGGCSAGNDIGPYGERDDQQVFIQKVVPSASQLFVRLSSTGQRVCSVRSVDGSPTTAFTVLECEGSRRLGSRPRRYLLTGQANGSLAMWDLTTAMDGLGQAPAGGLTEQELMEQLEHCELAPPAPSAPSWGCLPSPSPRISLTSLHSASSNTSLSGHRGSPSPPQAEARRRGGGSFVERCQELVRSGPDLRRPPTPAPWPSSGLGTPLTPPKMKLNETSF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein contains novel BTB domains that confer a unique function or activity.2. The engineered protein is expected to adopt a strand-strand-helix-helix-strand-helix-helix-helix-based conformation.3. Incorporating a PXXXPW, PXXXPR motif is critical for the successful design of the protein.4. A protein that can perform identical protein binding in lysosome to support positive regulation of epidermal growth factor receptor signaling pathway.5. For general function, the protein need meet that Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1; Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation.6. The protein design should emphasize Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "PPSAPRSVVHHLNGSTLRLEWSAPLESGGREDLTYAVRCRECRPGGSCLPCGGDMTFDPGPRDLVEPWVAIRGLRPDVTYTFEVAALNGVSTLATGPPPFEPVNVTTDRPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNKRASVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNTSVHITYKVLDEETLLNTKLETADLKWVTYPQAEGQWEELSGLDEEQHSVRTYEVCDMKRPGGQAHWLRTGWVPRRGAVHVYATIRFTMMECLSLPRASRSCKETFTVFYYESEADTATAHTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNIKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNKRASVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNTSVHITLKPKDPKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQPESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGEAGKRSYRLSMKVKAFPSPEVVWLKDGLPATEKSARYLTRGYSLIIKDVTEEDAGNYTILLSIKQ",
    "response#2": "EETLLNTKLETADLKWVTYPQAEGQWEELSGLDEEQHSVRTYEVCDMKRPGGQAHWLRTGWVPRRGAVHVYATIRFTMMECLSLPRASRSCKETFTVFYYESEADTATAHTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNIKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKPNGFHVNLEKMPTEGEDLKLSCTVNKFLYRDVTWILLRTVNNRTMHYSISKQKMAITKEHSITLNLTIMNVSLQDSGTYACRARNVYTGEEILQKKEIEAKEGVFPDNFAVQLSVKVAVRVRPMNRREKDLEAKFIIQMEKSKTTITNLKIPEGGTGDSGRERTKTFTYDFSFYSADTKSPDYVSQEMVFKTLGTDVVKSAFEGYNACVFAYGQTGSGKSYTMMGNSGDSGLIPRICEGLFSRINETTRWDEASFRTEVSYLEIYNERVRDLLRRKSSKTFNLRVREHPKEGPYVEDLSKHLVQNYGDVEELMDAGNINRTTAATGMNDVSSRSHAIFTIKFTQAKFDSEMPCETVSKIHLVDLAGSERADATGATGVRLKEGGNINKSLVTLGNVISALADLSQDAANTLAKKKQVFVPYRDSVLTWLLKDSLGGNSKTIMIATISPADVNYGETLSTLRYANRAKNTEFMENGALDSFLDEFAANMRTQVEIFVNRMKSNSSRGRSISNDSSVQTLFMTLTSLHSQQLSYIKEMDDKRMWYEQLQDKLTQIKDSRAALDQLRQEHVEKLRRIAE",
    "response#3": "ETLLNTKLETADLKWVTYPQAEGQWEELSGLDEEQHSVRTYEVCDMKRPGGQAHWLRTGWVPRRGAVHVYATIRFTMMECLSLPRASRSCKETFTVFYYESEADTATAHTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNIKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKPSAPLAGLAWSSASAPPPRGFSAISCTVEGAPASFGKSFAQKSGYFLCLSSLGSLENPQENVVADIQIVVDKSPLPLGFSPVCDPMDSKASVSKKKRMCVKLLPLGATDTAVFDVRLSGKTKTVPGYLRIGDMGGFAIWCKKAPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNKRASVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNTSVHIEETLLNTKLETADLKWVTYPQAEGQWEELSGLDEEQHSVRTYEVCDMKRPGGQAHWLRTGWVPRRGAVHVYATIRFTMMECLSLPRASRSCKETFTVFYYESEADTATAHTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNIKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKPKDGYLRFDACVADFPEKDCPVVVSAGNSELSLQLRLPGQQLREGSFRVTRMRCWRVTSSVPLPSGSTSSPGRGRGEVRLELAFEYLMSKDRLQWVTITSPQAIMMSICLQSMVDELPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNKRASVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNTSVHI"
  },
  {
    "reference": "MSKIGLFFGTQTGNTEELAQAIQAAFGGSDIVELFDVAEVDIEALRDFDQLIIGCPTWNVGELQSDWEALYDDLDDVDFSGKTIAYFGAGDQVGYADNFQDAMGVLEEKITSLGGKTVGQWPTAGYDHSESKAERDGKFVGLAIDEDNQPELTAERIQAWVAQLKPAFGL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should contain one or more Flavodoxin-like domains that are essential for its function. The designed protein sequence is ",
    "response#1": "FSTMTVREGEPITMSANAIGTPQPRITWQKDGVQISSTAERFVGIDGGATCLEIPRVTANDAGWYQCTAQNIAGSTANRARLYVR",
    "response#2": "EGKNFVITAGPTIEPIDPVRFLSNHSSGKMGYAIARRAKKRGANVTLISGPTNIEIPKVDNFVRIKTTQDMFDQVGKYFDNADVLIKAAAPADYKPKYYSKEKIKKNSSYDLNNIELEKNPDIAKYYGSKKSKQIIVGFAAESTNIYEYAKEKLNSKNLDMIVVNNIVREGAGFSADTNIASIE",
    "response#3": "EVVLGVSGGIAAYKACLLLRLLREAGHGVTVVPTRNALHFVGLDTWQALSGRPVSTDVWTGSEEVPHVRLGRAADLVVVAPATADLLSRAATGRADDLLTNVLLTTTSPVLMAPAMHTEMWQHPATVDNVALLRRRGVVVMDPDSGRLTGADTGPGRLPEPEELHSQVL"
  },
  {
    "reference": "MEPARVSVLGKDSVVLKFGLWKEYVANDLLTTLTSSTYVLVTDTNLSHLYIPPFRSSFERACSQVEVQPRLLTFEVPPGETSKSRQTKADIEDWLLSQKPPCGRDTVIIALGGGVIGDLTGFVAATYMRGVRYVQVPTTLLAMVDSSIGGKTAIDTALGKNLIGAIWQPQRIYIDLGFLGTLSQREFINGMAEVIKTAAISDQEEFGALEANAELITNTIKSDPRQGASRLESIKDILQRVILASVKFKAHVVTADEREGGLRNLLNFGHSIGHAIEAFLTPQILHGECVAIGMVKEAELARYLGVLKGVAVGRLVKCISAYGLPTTLEDDRVRRLSGGKHCSVEQLIMKMGIDKKNDGAKKKVVLLSAIGRTYEPRASTVSDAELRVALSPSIEVTPGVPSSLDVTCAPPGSKSISNRALVLAALGSGTCRLSNLLTSADTEVMMAALSHLGAATFTWEDEGEVLVVSGNGGNMLASSTDLYLGNAGTAARFLTTVATLAHQSEVNNSVLTGNARMKQRPIGDLVDALRTNGAGVEYLETNGCLPLRIAASGGFNGGIINLAAKVSSQYVSSLLMCAPYAKNPVTLKLVGGKPVSQPYIDMTVAMMASFGIKVEKSSTEDHTYHIPQGRYANPAEYVIESDASSATYPLAVAAITGTTCTVPNIGSASLQGDARFAVDVLRPMGCKVSQTPSSTTVTGPKDGNLTPLTGVDMEPMTDAFLTASVLGAVAQGKGPNHTTRIYGIANQRVKECNRILAMKDELSKFGVM",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should consider the endogenous proteins and enzymes present in the chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7, Metabolic intermediate biosynthesis pathway to avoid interference with native metabolic processes.2. The protein contains novel 3-dehydroquinate synthase, SDH C-terminal, Shikimate dehydrogenase substrate binding N-terminal domains that confer a unique function or activity.3. The designed protein should have a high specificity for NAD(+), Zn(2+), 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate binding, with minimal off-target effects.4. The designed protein must have a For EPSP synthase activity that is structurally stable and resistant to denaturation.5. The protein must bind to Zn(2+) in order to perform its enzymatic function.6. The designed protein's functionality will benefit from Polar residues compositional bias in its structure.7. The ideal protein would be one that plays a critical role in chorismate biosynthetic process, amino acid biosynthetic process through 3-dehydroquinate dehydratase activity, 3-phosphoshikimate 1-carboxyvinyltransferase activity, shikimate kinase activity within cytoplasm. The designed protein sequence is ",
    "response#1": "YTVSPGETSKSRQTKADIEDWMLSQQCTRDTVIIALGGGVIGDMIGYVAATFMRGVRFVQVPTTLLSMVDSSIGGKTAIDTPMGKNLIGAFWQPRRIYIDLAFLETLPVREFLTDSQALHPLVVAADIKRGIAEVIKTACIWNADEFARLEAHSDIFLNIVNNSKLIKVANEKTDEVQEITYTAIDQMLEHTYKLVLESIKVKAHVVSSDERESSLRNLLNFGHSIGHAYEAILTPQALHGECVSIGMIKEAELSRYLGILTSTQVARLYKILVAYGLPVSPDEKWFKELTLQKKTPLNVLLSKMSIDKGMAEVVKTAAIWNADEFAALESRSSEIFAAIQTPSQFNAGRTLSTRSEAQALLLQVIVGSISVKAHIVTIDERETGLRNLVNFGHTIGHAIEAVVTPQILHGECVSIGMILEAEIARLLGVLGQVAVGRLTRCLKAYNLPVSVHDPRIASLPTSKLLTIDRLLDIMKVDKKGMAEVVKTAAIWNADEFAALESRSSEIFAAIQTPSQFNAGRTLSTRSEAQALLLQVIVGSISVKAHIVTIDERETGLRNLVNFGHTIGHAIEAVVTPQILHGECVSIGMILEAEIARLLGVLGQVAVGRLTRCLKAYNLPVSVHDPRIASLPTSKLLTIDRLLDIMKVDKKR",
    "response#2": "GMAEVVKTAAIWDEVDFAKLESRAPAIKSAVLGPFPPNAPKDQGRTLATRTEAQTLLLDVIRGSVGVKAHIVNIDEKETGLRNLVNFGHSIGHAIEAVLTPDMLHGECISIGMILEAEIARFTNGLSQVAIGRLTRCLKEYNLPVTMADPRVSRLSRSSGLTVDRLLDIMRVDKKGADRIRLYTVSPGETSKSRQTKADIEDWMLSQQCTRDTVIIALGGGVIGDMIGYVAATFMRGVRFVQVPTTLLSMVDSSIGGKTAIDTPMGKNLIGAFWQPRRIYIDLAFLETLPVREFRALHATVAPPGSKSISNRALVMAALGKGTCRLRNLLRSDDTEFMLSAIAQLKGASYAWEEAGEVLAVRGNGGKLVASGEPLYLGNAGTASRFLTSVVALCGPCAASGVSSTVLTGNDRMKVRPIGPLVDALRSNGVHVTYLGKEKSLPLAIDAAGGLEGGTIELAATVSSQYVSSILMAAPYAKKPVVLRLVGGKPISQPYIDMTLSMMAAFGIRVEHSDSEPNTYRIPRGTYSNPAEYVVESDASSATYPLAVAAITGTTCTVPNIGSASLQGDAAFAVGVLRPMGCSVTQTATSTTVTGPPVGSLKAISHVDMEPMTDAFLTASVLAAVASGTTRITGIANQRGMAEVVKTAAIWNADEFAALESRSSEIFAAIQTPSQFNAGRTLSTRSEAQALLLQVIVGSISVKAHIVTIDERETGLRNLVNFGHTIGHAIEAVVTPQILHGECVSIGMILEAEIARLLGVLGQVAVGRLTRCLKAYNLPVSVHDPRIASLPTSKLLTIDRLLDIMKVDKK",
    "response#3": "YAIPPGEYSKGRETKAEIEDWMLSHQCTRDTVIIALGGGVIGDMIGYVAATFMRGVRFVQVPTTLLAMVDSSIGGKTAIDTPMGKNLIGAFWQPRRIYIDLEFLETLPVREFRALHATVAPPGSKSISNRALVMAALGKGTCRLRNLLRSDDTEFMLSAIAQLKGASYAWEEAGEVLAVRGNGGKLVASGEPLYLGNAGTASRFLTSVVALCGPCAASGVSSTVLTGNDRMKVRPIGPLVDALRSNGVHVTYLGKEKSLPLAIDAAGGLEGGTIELAATVSSQYVSSILMAAPYAKKPVVLRLVGGKPISQPYIDMTLSMMAAFGIRVEHSDSEPNTYRIPRGTYSNPAEYVVESDASSATYPLAVAAITGTTCTVPNIGSASLQGDAAFAVGVLRPMGCSVTQTATSTTVTGPPVGSLKAISHVDMEPMTDAFLTASVLAAVASGTTRITGIANQRLAGG"
  },
  {
    "reference": "MTELVLATRNRDKARELTALLNGLAFRIRTLDDFPQAPDVVEDGATCEANAIKKATEIARATGRYAVADDTGLEVDALGGRPGAYAARYAGPGATYEDNCRKLLHELEGIPQERRTARFVTVAALASPSGEIQTAHGVLEGAIADRCRGERGFGYDPVFYVPELEKTLAELTPEEKNRISHRAKAFLKLRDLLQSRA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit XTP diphosphatase activity, ribonucleoside triphosphate phosphatase activity.2. We need a protein that can perform dITP + H2O = dIMP + diphosphate + H(+) with high efficiency. The designed protein sequence is ",
    "response#1": "LTIPAT",
    "response#2": "MPK",
    "response#3": "GMIERL"
  },
  {
    "reference": "MSRLREKYEKEVVPALMERFGYKNIMQVPKVEKVVINIGVGEAKENPKALEAAVDDLTMIAGQKPVITRAKRSIANFKIRKGMPIGVKVTLRGERMYEFMDKLFNIALPRVRDFKGVSPNSFDGRGNYALGIKEQLIFPEIDYDKIDKIRGMDIIIVTTAKTDEEAKGLLELLGMPFAK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein design must be able to perform structural constituent of ribosome in order to contribute to translation.2. The protein must exhibit the following characteristics: In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. The designed protein sequence is ",
    "response#1": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#2": "MTVISGQKPVKTLARKR",
    "response#3": "PIGVKVTLRGKKAEEFLNKVLAAVGHRIKASSFDEYGNVSFGIAEHVLIPGTRYDPEIGIFGMDVAITLVRPGFRVARRRRKKAHIPKRHRTVSKEEAMEI"
  },
  {
    "reference": "MESVAPERLDSAALPLVPAGNGAGRPSLVGADLAGLGDAVQALGEPAFRAKQLRHWIYHRGARSFEEMTSISKAARSKLAERYTLGRPAPADMQVSSDGTRKWLLRFADGTEAEMVFIPEEDRGTLCISSQVGCTLNCRFCHTGTQPWVRNLDAGEIVGQLMLARDALGDWPSGKRDRKITNIVLMGMGEPLYNYDNVARAVRIMIDGDGIAISRRRVTLSTAGVVPVMRQCGEELGVNLAVSLHAVTDDIRSEIVPLNRRYPIAELLDACRNYGGVNNARRITFEYVMLKGVNDSDADARELVRLLRDIPAKINLIPFNPWPGAPYECSSERRIRAFMTICLDGGLTCIVRTPRGRDIMAACGQLKSASERKRAGERS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be involved in rRNA base methylation by localizing to cytoplasm and exhibiting metal ion binding, rRNA binding, rRNA (adenine-C2-)-methyltransferase activity.2. The protein contains novel Radical SAM core domains that confer a unique function or activity.3. The protein with the reaction adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine, the Proton acceptor, S-methylcysteine intermediate must be optimized to ensure efficient catalysis.4. The [4Fe-4S] cluster binding site should be stable and able to withstand changes in environmental conditions.5. The protein must exhibit the following characteristics: m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.; Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.6. [4Fe-4S] cluster is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "MDKRPLIHGLSKSEIGQIVASLGQPSYRATQIWEWLYKRMAFEWNAMSNLPLALREHLDRELAICPGTLTARSADASGSEKLLLTLRDGECVEQVLIPAPGRKTVCVSSQVGCRFKCAFCASGQAGFRRNLEAGEIVAQTLWAALIWERRPSNVVFMGIGEPLDNYDNVLKAIRIINDSEGLGIGARKITISTSGIIPGMERLAGEGLQIELSVSLHAPTQELRSRLMPVARLYPLDRLIRACRSYFEKTKRLITFEYVLIRGTNDQASHARKLAELLSDFPCRVNLLLLSPVPEFKGTSSSPDAANAFIKTLSRAGINATLRAPRGLSVNAACGQLRSSEP",
    "response#2": "DKRPLIHGLSKSEIGQIVASLGQPSYRATQIWEWLYKRMAFEWNAMSNLPLALREHLDRELAICPGTLTARSADASGSEKLLLTLRDGECVEQVLIPAPGRKTVCVSSQVGCRFKCAFCASGQAGFRRNLEAGEIVAQTLWAALIWERRPSNVVFMGIGEPLDNYDNVLKAIRIINDSEGLGIGARKITISTSGIIPGMERLAGEGLQIELSVSLHAPTQELRSRLMPVARLYPLDRLIRACRSYFEKTKRLITFEYVLIRGTNDQASHARKLAELLSDFPCRVNLLLLSPVPEFKGTSSSPDAANAFIKTLSRAGINATLRAPRGLSVNAACGQLRSSALG",
    "response#3": "INLLDLTLDELKQWMSENGENSFRAKQVLDWIYKGVFEFQQMNNIPKSSREKFNEVFTISIPRVLEKMVSKKDETRKYLLIYEDGNIIESVVMKYVYGYSICISTQIGCRMGCKFCASTIDGRVRDLTAGELIGEILAVQKDLGQRISNIVLMGSGEPLDNYENVIKFIKMVNEKYSLNIGQRHITLSTCGIVPKIYDLAREGLQITLAISLHAPNDIIRKQSMPVANSYSIDELIKSCQFYIDKTNRRVTFEYALIKGLNDSPSCAEELSKMLKGLLCHVNLIPINEVKEREYIKADKEIINEFKSILLKNGIEATLRREMGGDINAACGQLRRSYL"
  },
  {
    "reference": "MVMNPDAVRNVVGIIGNLISFGLFLSPLPTFVTIVKKKDVEEFVPDPYLATFLNCALWVFYGLPFIHPNSILVVTINGTGLLIEIAYLAIYFAYAPKPKRCRMLGVLTVELVFLAAVAAGVLLGAHTYDKRSLIVGTLCVFFGTLMYAAPLTIMKQVIATKSVEYMPFTLSLVSFINGICWTIYAFIRFDILITIPNGMGTLLGAAQLILYFCYYDGSTAKNKGALELPKDGDSSAV",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Mediates both low-affinity uptake and efflux of sugar across the plasma membrane ; Involved in regulating the crosstalk between sugar and auxin; Can transport galactose; Regulates negatively the auxin signaling pathway and translocation.; Prevents growth but promotes senescence2. The Cytoplasmic, Extracellular topological domain of the protein should be designed to be flexible or rigid.3. The designed protein should have at least Helical; Name=2, Helical; Name=5, Helical; Name=6, Helical; Name=3, Helical; Name=7, Helical; Name=4, Helical; Name=1 transmembrane domains to ensure proper localization.4. A protein that can perform sugar transmembrane transporter activity in membrane, plasma membrane to support galactose transmembrane transport, carbohydrate transport, auxin-activated signaling pathway, auxin transport, negative regulation of auxin mediated signaling pathway.5. The protein contains novel MtN3/slv domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "ALSIATIGSFQFGYNTGVINAPETIIKDFLNYTLEEKSENLPTEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLMVNLLAVAGGCLMGFCKIAQSVEMLILGRLIIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVIGILVAQIFGLKVIMGTEELWPLLLGFTIIPAVLQSAALPFCPESPRFLLINRKEEENAKEILQRLWGTQDVSQDIQEMKDESARMAQEKQVTVLELFRSRSYRQPIIISIMLQLSQQLSGINAVFYYSTGIFKDAGVEEPIYATIGAGVVNTIFTVVSLFLVERAGRRTLHMIGLGGMAVCSILMTISLLLKDNYNWMSFVCIGAILVFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAFYLGAYVFIIFTGFLIVFLVFTFFKVPETKGRTFDEIASGFRQGGASQSDKTPEDQFHPLGADSQV",
    "response#2": "ALSIATIGSFQFGYNTGVINAPETIIKDFLNYTLEEKSENLPTEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLMVNLLAVAGGCLMGFCKIAQSVEMLILGRLIIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVIGILVAQIFGLKVIMGTEELWPLLLGFTIIPAVLQSAALPFCPESPRFLLINRKEEENAKEILQRLWGTQDVSQDIQEMKDESARMAQEKQVTVLELFRSRSYRQPIIISIMLQLSQQLSGINAVFYYSTGIFKDAGVEEPIYATIGAGVVNTIFTVVSLFLVERAGRRTLHMIGLGGMAVCSILMTISLLLKDNYNWMSFVCIGAILVFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAFYLGAYVFIIFTGFLIVFLVFTFFKVPETKGRTLEQITAHFEGRVGILGNAASMLLYAAPILTFRRVIKKGSVEEFSCVPYILALFNCLLYTWYGLPVVSSGWENSTVSSINGLGILLEIAFISIYTWFAPRHGGSVYDAWFSCASNQVAQVLLTLPYSFSQLGMASGVAFQVFYGLMGSWTAYLISVLYVEYRTRRERDKVDFRNHVIQWFEVLDGLLGRHWRNAGLLFNCTFLLFGSVIQLIACASNIYYINDRLDKRTWTYIFGACCATTVFVPSFHNYRVWSFLGLLMTSYTAWYLTVAAVVHGKVDGAAPRAGPSKTMVLYFTGATNILYTFGGHAVTVEIMHAMWRPRRFKMIYLAATAYVLTLTLPSAAAMYWAFGDALLDHSNAFALLPRTPWRDAAVVLMLIHQFITFGFACTPLYFVWEKAIGVHGGAGVLRRAAARLPVVLPIWFLAVIFPFFGPINSTVGSFLVSFTVYIIPAMAHMATFAPAAARENAVEP",
    "response#3": "ALSIATIGSFQFGYNTGVINAPETIIKDFLNYTLEEKSENLPTEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLMVNLLAVAGGCLMGFCKIAQSVEMLILGRLIIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVIGILVAQIFGLKVIMGTEELWPLLLGFTIIPAVLQSAALPFCPESPRFLLINRKEEENAKEILQRLWGTQDVSQDIQEMKDESARMAQEKQVTVLELFRSRSYRQPIIISIMLQLSQQLSGINAVFYYSTGIFKDAGVEEPIYATIGAGVVNTIFTVVSLFLVERAGRRTLHMIGLGGMAVCSILMTISLLLKDNYNWMSFVCIGAILVFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAFYLGAYVFIIFTGFLIVFLVFTFFKVPETKGKTFVEINRIFTKMNKHGGSVYDAWFSCASNQVAQVLLTLPYSFSQLGMASGVAFQVFYGLMGSWTAYLISVLYVEYRTRRERDKVDFRNHVIQWFEVLDGLLGRHWRNAGLLFNCTFLLFGSVIQLIACASNIYYINDRLDKRTWTYIFGACCATTVFVPSFHNYRVWSFLGLLMTSYTAWYLTVAAVVHGKVDGAAPRAGPSKTMVLYFTGATNILYTFGGHAVTVEIMHAMWRPRRFKMIYLAATAYVLTLTLPSAAAMYWAFGDALLDHSNAFALLPRTPWRDAAVVLMLIHQFITFGFACTPLYFVWEKAIGVHGGAGVLRRAAARLPVVLPIWFLAVIFPFFGPINSTVGSFLVSFTVYIIPAMAHMATFAPAAARENAVEP"
  },
  {
    "reference": "MLAGKRIVLGVTGGIAAYKAVEVSRRLVDAGAHVVPVMTAGAEHFIGRTTLSALASEPVQTSLWDEASPIPHTMLGQTADLIVVAPATARLLAAYAAGLSTDLLTNVLLATRAPVVVCPAMHTEMWEHPAVQANIATLRSRGVHIVEPEEGRLAGGDVGAGRLAAPERIVAAIEAVLSAGDDLAGLHVVVSAGGTREPIDAVRVIANRSSGKQGYAVAAEAAARGARVTLVSTVDLPVPGGVVVRTVETAAQMHAVLDELAPTADVIVMAAAVADFRPVTVAAGKLKKDQGIPQIMLEPTPDILAGLGASKPAGQVLVGFAAETSDLLANAESKLRRKNLDLIVANDVAAPGVGFQHDTNAVTLLQRGGEVHTIALTDKRSIAQAILTIVVGIRAAGTIPNSKRSLTK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein with phosphopantothenate--cysteine ligase activity functions is required to enable pantothenate catabolic process, coenzyme A biosynthetic process.2. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.3. The presence of DNA/pantothenate metabolism flavoprotein C-terminal domains is critical for the interaction of the designed protein with its intended targets.4. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.5. A protein that is capable of catalyzing reactions within the coenzyme A biosynthesis pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "MSGKHVLITLGPTREKWDAVRFWSNPSSGLMGACIAMAAWLRGAKVTVVSGPVNWWFPEDINVIKVNSAQQMFDAATEVWPSCTTGCFTAAVADFKPIPHGEGKFKKEGSSALRVDFDTNPDILKTIGSMKKDDQQLIGFAAETSNIQEAAKGKLERKNLDLIVANPINKPGAGFESSTNSVYLTGKGVLISAGGTREYLDPVRFIGNPATGKMGLALAQAALHRGANVTLVYCPATWDIPLGVQAIPVVSAEQMQQVMQSYLHNAEIIIMSAAVADVKPKDYSTEKLPKRSLPQALPLEPVPDIIAQLAKLKQPHQLLIGFAAQTGDIITPAMAKLHNKNLDIIVANPIDQPDSGFGSDNNQAIDCLKL",
    "response#2": "MSGKHVLITLGPTREKWDAVRFWSNPSSGLMGACIAMAAWLRGAKVTVVSGPVNWWFPEDINVIKVNSAQQMFDAATEVWPSCTTGCFTAAVADFKPIPHGEGKFKKEGSSALRVDFDTNPDILKTIGSMKKDDQQLIGFAAETSNIQEAAKGKLERKNLDLIVANPINKPGAGFESSTNSVYLAGLNVVISSGPTREPLDGVRFLSNPATGRMGHALAEAAIHRGATVTLVSGPTHLPDPAGARVVHVTRVEEMRHAILEGSEGSDLVVMAAAVGDYRLARPAAGKLKKSARGLDLHLEPAPDILAELGRRRGSAPRPVLVGFAAETDDLVLNARRKLEAKNLDHIVANPVNRAGAGFGAETNQVTLITAEEEMDLPVMPKTGVAAAILDRIIELVSG",
    "response#3": "MSGKHVLITLGPTREKWDAVRFWSNPSSGLMGACIAMAAWLRGAKVTVVSGPVNWWFPEDINVIKVNSAQQMFDAATEVWPSCTTGCFTAAVADFKPIPHGEGKFKKEGSSALRVDFDTNPDILKTIGSMKKDDQQLIGFAAETSNIQEAAKGKLERKNLDLIVANPINKPGAGFESSTNSVYSSS"
  },
  {
    "reference": "MAGLAKAAQAQLKQALAAPAARLFSSASAAPYPASSSQPPAPGGSGNGENGAGAANGQAKPKLTKAQRFTLDPATASTPPKFPHGFLATGLHCGVKKDRSKLDLAVIISDRPCSAAGTFTRNAFVAAPVIVSQEVLARQRGIARGLVVNSGCANAVTGSKGLEDARAMSDQLDSFLSASSSSDGTGTLVMSTGVIGQLLPIEKIRQGIEASTATLGSTFSAWSDCARAFMTTDTFPKLRTRTIQLGGKEVRMVGIDKGAGMIHPNMGPPAAPHATMLGVIATDAAVEPASLQNALTYAVDRSFNSISVDGDMSTNDTVLVLANGASGMAEISEKKTPEEFVRFREELTSFAAELAQLVVRDGEGAEKFVKVTVQGAPSYEGAHKIASTVSTSSLVKCALHGEDANWGRILCAVGYSQPPFPIDPTKVTVSFVPQDGSPELKLLVNGEPENVDEERASEILREDDLEIKIDLGLGEETATYWTCDLSHEYISINADYRS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that contributes to arginine biosynthetic process by functioning in mitochondrial matrix with methione N-acyltransferase activity, glutamate N-acetyltransferase activity.2. For general function, the protein need meet that Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate.3. We require a protein that can specifically bind to the metabolites produced in the N(2)-acetyl- L-ornithine from L-glutamate: step 1/4, Amino-acid biosynthesis pathway.4. The designed protein should have a binding site that is specific to the target substrate ligand.5. The Nucleophile of the protein must be engineered to enable effective catalysis of acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate. The designed protein sequence is ",
    "response#1": "MRGPFLNICTALLLLTSCSSSTSSSSTPSSGVVIVTTEVLGDIAKSILGDQGTVTSIMPTGANPHSFEPKPSDIAKIKDADLFIYNGLHLEGWMERLLDASGKVSSLGEGVSGAELLAASGEESTHNLWFDINLWQRSVEKISAALCEFDKENSQHYSSNAKVYIKKLASLDKEIGDLLSQYAGSKRIALTYHDAYGYFAKRYGLSVIGAIQPEPGIQPGAKDLKALIDKIKSEQVGCVFSEPQFSSKSGETIARESGVKVLPLSDLVGGKPGKDSYFELMRYNVETIAKALNG",
    "response#2": "MKNTYPIEQSNNRWFXXIXGXPXDXPXXAXNXXAXPXRXLXPXKXPXRXXAVXGXDX",
    "response#3": "MRDPAHDGRPVAAVPRGGAAPEGTGRIPRSWEMAPDAGTGLADPDGVAAVRAAFGDFTPVRQERSSTGPDWLTDLPGDGTGLVFGKREISGRPVAFVKQTMEASGPAGPAAVTGLFPEEPVRVPDAGGRFARLHALYLADPSRALPGGAAMDGHPVPGRGMFAGVHPKATVLHPTAVIGEGARVGDNVTVGAHCVIGPDVVIGDNTVIHSNVVIGEDGFGYAYDAQGRHEKIPQVGRVIIGNDVEIGANTTIDRGALRDDVIGDGTKIDNLVQIGHNCRIGEVSTIVGGTGVAGSTKIGRHVVAGAAGGVAGHLTIGDGAHVTGMTMVTRSIEPGAQFSGTPAISIPEYLRATTSLARMPGMFKRLAARMEALEKQ"
  },
  {
    "reference": "MPLHVKWPFPAVPPLTWTLASSVVMGLVGTYSCFWTKYMNHLTVHNKEVLYELIENRGPATPLITVSNHQSCMDDPHLWGILKLRHIWNLKLMRWTPAAADICFTKELHSHFFSLGKCVPVCRGELVSVLEGPMGVGIPGANMRGQRLWEEGWKGMDFILEKLNHGDWVHIFPEGKVNMSSEFLRFKWGIGRLIAECHLNPIILPLWHVGMNDVLPNSPPYFPRFGQKITVLIGKPFSALPVLERLRAENKSAVEMRKALTDFIQEEFQHLKTQAEQLHNHLQPGR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a fast turnover rate for 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-   phosphocholine + 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1,2-   dihexadecanoyl-sn-glycero-3-phosphocholine + 2-(9Z,12Z-   octadecadienoyl)-sn-glycero-3-phosphocholine.2. A protein that localizes to mitochondrial outer membrane and can perform O-acyltransferase activity with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "DPVKGRGIRILAIDGGGTRGVVALQTLRKLVELTQKPIHQLFDYICGVSTGAILAFMLGLFHMPLDECEELYRKLGSDVFTQNVIVGTVKMSWSHAFYDSHTWEKILKDKVGSALMIETARDPLCPKVAAVSTIVNRGQTPKAFVFRNYGHFPGTNSHYLGGCQYKMWQAIRASSAAPGYFAEYALGNDLHQDGGLLLNNPSALALHECKCIWPDTPLECIVSLGTGRYESDVRNTTTYTSLKTKLSNVISSATDTEEVHIMLDGLLPADTYFRFNPVICENIPLDESRNEKLDQLQLEGMKYLERDPVKGRGIRILAIDGGGTRGVVALQTLRKLVELTQKPIHQLFDYICGVSTGAILAFMLGLFHMPLDECEELYRKLGSDVFTQNVIVGTVKMSWSHAFYDSHTWEKILKDKVGSALMIETARDPLCPKVAAVSTIVNRGQTPKAFVFRNYGHFPGTNSHYLGGCQYKMWQAIRASSAAPGYFAEYALGNDLHQDGGLLLNNPSALALHECKCIWPDTPLECIVSLGTGRYESDVRNTTTYTSLKTKLSNVISSATDTEEVHIMLDGLLPADTYFRFNPVICENIPLDESRNEKLDQLQLEGMKYLERLLG",
    "response#2": "DPVKGRGIRILTIDGGGTRGVVALQTLRKLVELTQKPVHQLFDYICGVSTGAILAFMLGLFHLPLDECEELYRKLGSDIFSQNVIVGTVKMSWSHAFYDSQTWEKILKERMGSALMIETARNPMCPKVAAVSTIVNRGSTPKAFVFRNYGHFPGSQSHYLGGCQYKMWQAIRASSAAPGYFAEYALGNDLHQDGGLLLNNPSALAMHECKCLWPDAPLECIVSLGTGRYESDVRNNTTYTSLKTKLSNVINSATDTEEVHIMLDGLLPPDTYFRFNPVMCENIPLDESRNEKLDQLQLEGSKYIERIVATFP",
    "response#3": "DPVKGRGIRILTIDGGGTRGVVALQTLRKLVELTQKPVHQLFDYICGVSTGAILAFMLGLFHLPLDECEELYRKLGSDIFSQNVIVGTVKMSWSHAFYDSQTWEKILKERMGSALMIETARNPMCPKVAAVSTIVNRGSTPKAFVFRNYGHFPGSQSHYLGGCQYKMWQAIRASSAAPGYFAEYALGNDLHQDGGLLLNNPSALAMHECKCLWPDAPLECIVSLGTGRYESDVRNNTTYTSLKTKLSNVINSATDTEEVHIMLDGLLPPDTYFRFNPVMCENIPLDESRNEKLDQLQLEGSKYIERLTIDGGGTRGVVALQTLRKLVELTQKPVHQLFDYICGVSTGAILAFMLGLFHLPLDECEELYRKLGSDIFSQNVIVGTVKMSWSHAFYDSQTWEKILKERMGSALMIETARNPMCPKVAAVSTIVNRGSTPKAFVFRNYGHFPGSQSHYLGGCQYKMWQAIRASSAAPGYFAEYALGNDLHQDGGLLLNNPSDSEVDLTIDGGGTRGVVALQTLRKLVELTQKPVHQLFDYICGVSTGAILAFMLGLFHLPLDECEELYRKLGSDIFSQNVIVGTVKMSWSHAFYDSQTWEKILKERMGSALMIETARNPMCPKVAAVSTIVNRGSTPKAFVFRNYGHFPGSQSHYLGGCQYKMWQAIRASSAAPGYFAEYALGNDLHQDGGLLLNNPSDSEVDDKVRLTIDGGGTRGVVALQTLRKLVELTQKPVHQLFDYICGVSTGAILAFMLGLFHLPLDECEELYRKLGSDIFSQNVIVGTVKMSWSHAFYDSQTWEKILKERMGSALMIETARNPMCPKVAAVSTIVNRGSTPKAFVFRNYGHFPGSQSHYLGGCQYKMWQAIRASSAAPGYFAEYALGNDLHQDGGLLLNNPS"
  },
  {
    "reference": "MIDIAGQPVRTATQMREAEARAFAAGRGADALMAQAGEAVAIAARRFAGSDPILVLCGPGNNGGDGYVAAGILGRMGHPVRVAAIGEPRTEVAMRARAGWGGPVEGIEDAAPAGVVIDAILGIGMERPLAATWNDALARMRNAARFMIAVDVPTGVDSDVGTVPPWLCNASVDLTLALGAVKPAHLLQPSAARCGVVRLVDLGLVPQSVDVRVAGRPTLPEPGPQSHKYSRGMVAIVTGGMAGAARLAGEAALRSGAGYVAHYGDTGQGGPAAIVHRDYDPARLDDPRIGAIVVGPGLGRDDRARAKVAAVLEKPRPLLVDGDALHLVTVDALRARAAPTILTPHAGEFRALFGEDAGTTIARARAAAVRSGCIVVLKGPTTVVADAAGAVVTPPGNPWLSTAGTGDLLAGAIAAQLAHPDADAFAAATAGVWLHGRAARIAGRSFIADDLARALTRARAGA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein requires Mg(2+) to activate a critical catalytic site.2. The designed protein have YjeF N-terminal domains with specific sequence motifs that are crucial for its activity.3. The protein have enhanced (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.4. The designed protein should be optimized for NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, NADPHX epimerase activity, ATP binding activity in order to improve nicotinamide nucleotide metabolic process.5. The K(+), AMP, (6S)-NADPHX binding site should be located in a region of the protein that is accessible to the ligand.6. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.. The designed protein sequence is ",
    "response#1": "MVIALCGKGNNAGDAYLVAMLAKLKGLEAQCIHFVAPNTLSGDAKSAYEQCHETEVHIAAYNCDTFRILLDKQTSDCIIVDGLFGTGLNRDLDANYADAIELSNTVRREKSNIHTISLDIPSGLSANTGRIFNACIEADLTTTFIANKIGLMTGVGRHYSGQIAFHDLNLIEGIFGELEFDAWLVENSHKGTFGTLAILAGSADMPGAAYLCATGALHAGVGLVKLASDASALGVLKNRLSEPVFLSGGAEAAAQTSKTALLVGCGLGRGFDASLGALLRGSDVPTVIDADGINFLASHIDVLKEMQAPAALTPHPMELSRLTGCGVEEINRNRVRTARAFAAEHGCFLLLKGSRTVIAAPDGRVRITVSGCSALAKGGSGDVLAGVVGALAAQGYDLFDALTVGAFLHGRAGERLAARFGAHGALPSQLPEEVVGSTAYPGAAVLSALGALGVGTGMVRHVAPPAVVALVHQHAPEVVAAPGRVQAWVLGSGVDPAAETKAGGDGQVAAIESALAASEPCVVDAGALDLIGRREDSPVRVLTPHAGELARLLTRLEIRVEGAGAGETGAAPGAPTDPVTRDQVLAAPVAHAQAAADELKAVVLLKGATTLVVCPSSWGQPVCAQADAPPWLATAGAGDVLGGILGALLASGLEPADAAALAALVHGVAADQL",
    "response#2": "MTAIERASEKAGVSTDVLMENAGLAVARQARALVGAAGVQVLVLVGPGNNGADGLVAARHLRRWGAQVTCYVVAGRPDPDAKKDRALEYGVRIIDAAEIGALERLLARSALVIDAVLGTGRARPLAGQVKETMISLAKARSSPTSPKLLALDLPTGLNADTGEVDPFGVPCDLTLALGYPKAGLLNFPGTGHAGELRVLDIGVPPGLPEAAELRLVLGGSPGLTGAPVMASLAAARIGAGFVTCACASSIQPVLALKMTAITTLALPETADGGLDPDAALAALAPRLEKARALLVGPGLGRHPATQTFIRRLLTRTDLPTVIDADGLNALAGHLDDLAEQAAGRWILTPHAGEFRRLAGTDADLTDRIRTVQTCARRWNSVLLLKGLPGLVGTPDGRVYLNPTGNPAVATAGTGDVLAGLCVGLLAQGLPPARAAVAALYAGGAAADAYTTHRHPHTMTPLDLLEELPDDATITGWMPQRDRHAHKYSVGLALVLGGSPGLTGAPVMASLAAARIGAGFVTCACASSIQPVLALKMTAITTLALPETADGGLDPDAALAALAPRLEKARALLVGPGLGRHPATQTFIRRLLTRTDLPTVIDADGLNALAGHLDDLAEQAAGRWILTPHAGEFRRLAGTDADLTDRIRTVQTCARRWNSVLLLKGLPGLVGTPDGRVYLNPTGNPAVATAGTGDVLAGLCVGLLAQGLPPARAAVAALYAGGAAADAYTTHRHPHTMTPLDLLEELPRVLHTRLA",
    "response#3": "MVIALCGKGNNAGDAYLVAMLAKLKGLEAQCIHFVAPNTLSGDAKSAYEQCHETEVHIAAYNCDTFRILLDKQTSDCIIVDGLFGTGLNRDLDANYADAIELSNTVRREKSNIHTISLDIPSGLSANTGRIFNACIEADLTTTFIANKIGLMTGVGRHYSGQIAFHDLNLIEGIFGKPEVLPMYRPRLKFSHKGTYGHAVIVGGSYGKIGAVQLASNACLTVGSGLVTAFVPECGYHSLQTAIPEIMVLTDEEDNTITKIDIPFKPSVVGIGMGMGTEEDTVKAFSALLKNIETPMVIDADGLNILAKHPKFMKDVPKQSVLTPHPKELERLIGEWEDDFDKLEKAKTFATKHDCILLIKGAHSITIYNTIGFVNTTGNPGMATAGSGDVLTGMITGLIAQGYSSLNAAVFGVYLHGLAGDMGVSHNGYEALKASGIIENIGKAYMETPVFCRQHLPPRDPSSHKGTYGHVLALGGSPGFTGAITLTARAALRAGAGLVTAAVPRGCQPVVAAGAPEIMTWGLPETAGGSLGRSALDPLLERLENFSVLAIGPGLGRDPETVELVKSLLPRASIPLVVDADALNALATDTGILSQPHGPMVLTPHPGEMGRLIGCPAAKIQEDRLNIARRYAQEWRCVLLLKGARTVVACPDGRAYINPTGNPGMATGGSGDVLTGIIAGLIAQGMSLDKAAAIGAYLHGAAGDLAAEELGQEALIAGDILHFLPRAFKSS"
  },
  {
    "reference": "MKAVIFFCLLSVMVFTVIEAVKEEGTKPAEAARECAAKNKRCADWAGPWCCEGLYCSCRSYPGCMCRPNSG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Incorporate a signal peptide in the protein design.2. The protein can be targeted to extracellular region and perform toxin activity.3. For general function, the protein need meet that The voltage dependence of activation is shifted toward more hyperpolarized potentials (analogous to site 4 toxins) and a non-inactivating persistent sodium current is induced (site 3-like action); Interestingly, both effects take place in a voltage-dependent manner, producing a bell-shaped curve between -80 and 0 mV; Insecticidal neurotoxin that modulates the insect Nav channel (DmNaV1/tipE (para/tipE)) in a unique manner, with both the activation and inactivation processes being affected The designed protein sequence is ",
    "response#1": "KDGYIVDDRNCTYFCGTNAYCNEECVKLKGESGYCQWVGRYGNACWCYKLPDHVRTVQAGRCR",
    "response#2": "KDGYIVDDRNCTYFCGTNAYCNEECVKLKGESGYCQWVGRYGNACWCYKLPDHVRTVQAGRCR",
    "response#3": "KDGYIVDDRNCTYFCGTNAYCNEECVKLKGESGYCQWVGRYGNACWCYKLPDHVRTVQAGRCR"
  },
  {
    "reference": "MSDVNPNPLPLVFEQPKGRGKPPRHLADLDLDARKEAVEALGLPGYRAKQLSTHYFTRLVSDPSEMTDLPAGIRDQLVEGMLPTLINPVRTLEADRGTTRKTLWRLFDGALVESVLMRYPDRATICVSSQAGCGMACPFCATGQGGLQRNMSTGEIIEQVVDGARAMARGEVPGGPGRLSNVVFMGMGEPMANYKAVIGAVRRMVDDGPSGLGMSARNITVSTVGLVPRIKQLTTEGIPVTLALSLHAPDDELRDDLVPINTRFKVAEAVEAAWEYARTTKRRVSIEYALIKDINDQAWRADMLGDLLRSYGDWGWVHVNLIPLNPTPGSIWTASRPEDEREFVRRLEAKGIPTTVRDTRGQEIDGACGQLAAADA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein requires [4Fe-4S] cluster to activate a critical catalytic site.2. The designed protein should be able to compete with endogenous proteins or inhibitors for [4Fe-4S] cluster binding.3. The protein must have high specificity towards rRNA binding, metal ion binding. The designed protein sequence is ",
    "response#1": "SGGASLQVAKNILESSFKLEAGTSSMGAVVSLVQTPGLHSPAYLAEVARVLVSGGDLIVQEPLLAEAQEQKCSSAQTKAQLERNLLLAGFVNLEVVDSVVGVEIAKACTTSSVALNVVAVKSSKPSWDTGC",
    "response#2": "SGGASLQVAKNILESSFKLEAGTSSMGAVVSLVQTPGLHSPAYLAEVARVLVSGGDLIVQEPLLAEAQEQKCSSAQTKAQLERNLLLAGFVNLEVVDSVVGVEIAKACTTSSVALNVVAVKSSKPSWDTGL",
    "response#3": "SGGASLQVAKNILESSFKLEAGTSSMGAVVSLVQTPGLHSPAYLAEVARVLVSGGDLIVQEPLLAEAQEQKCSSAQTKAQLERNLLLAGFVNLEVVDSVVGVEIAKACTTSSVALNVVAVKSSKPSWDTGC"
  },
  {
    "reference": "MKNIYCLIPKLVNFATLGCLWISVVQCTVLNSCLKSCVTNLGQQLDLGTPHNLSEPCIQGCHFWNSVDQKNCALKCRESCEVGCSSAEGAYEEEVLENADLPTAPFASSIGSHNMTLRWKSANFSGVKYIIQWKYAQLLGSWTYTKTVSRPSYVVKPLHPFTEYIFRVVWIFTAQLQLYSPPSPSYRTHPHGVPETAPLIRNIESSSPDTVEVSWDPPQFPGGPILGYNLRLISKNQKLDAGTQRTSFQFYSTLPNTIYRFSIAAVNEVGEGPEAESSITTSSSAVQQEEQWLFLSRKTSLRKRSLKHLVDEAHCLRLDAIYHNITGISVDVHQQIVYFSEGTLIWAKKAANMSDVSDLRIFYRGSGLISSISIDWLYQRMYFIMDELVCVCDLENCSNIEEITPPSISAPQKIVADSYNGYVFYLLRDGIYRADLPVPSGRCAEAVRIVESCTLKDFAIKPQAKRIIYFNDTAQVFMSTFLDGSASHLILPRIPFADVKSFACENNDFLVTDGKVIFQQDALSFNEFIVGCDLSHIEEFGFGNLVIFGSSSQLHPLPGRPQELSVLFGSHQALVQWKPPALAIGANVILISDIIELFELGPSAWQNWTYEVKVSTQDPPEVTHIFLNISGTMLNVPELQSAMKYKVSVRASSPKRPGPWSEPSVGTTLVPASEPPFIMAVKEDGLWSKPLNSFGPGEFLSSDIGNVSDMDWYNNSLYYSDTKGDVFVWLLNGTDISENYHLPSIAGAGALAFEWLGHFLYWAGKTYV",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein contains novel Protein kinase domains that confer a unique function or activity.2. The protein design should prioritize the formation of helix-strand-turn-strand-turn-strand-helix-strand-strand-strand-helix-helix-helix-strand-strand elements. The designed protein sequence is ",
    "response#1": "QIVTTQYGKVKGTTENGVHKWKGIPYAKPPVGQWRFKAPEPPEVWEDVLDATAYGSICPQPSDLLSLSYTELPRQSEDCLYVNVFAPDTPSKNLPVMVWIHGGAFYLGAGSEPLYDGSKLAAQGEVIVVTLNYRLGPFGFLHLSSFNEAYSDNLGLLDQAAALKWVRENISAFGGDPDNVTVFGESAGGMSIAALLAMPAAKGLFQKAIMESGASRTMTKEQAASTSAAFLQVLGINEGQLDKLHTVSAEDLLKAADQLRIAEKENIFQLFFQPALDPKTLPEEPEKAIAEGAASGIPLLIGTTRDEGYLFFTPDSDVHSQETLDAALEYLLGKPLAEKVADLYPRSLESQIHMMTDLLFWRPAVAYASAQSHYAPVWMYRFDWHPKKPPYNKAFHALELPFVFGNLDGLERMAKAEITDEVKQLSHTIQSAWITFAKTGNPSTEAVNWPAYHEETRETLILDSEITIENDPESW",
    "response#2": "CEKTGLESGGVAHGGALNAAQVAHLDEDAFKGGLHRPKFDSEGLHKPHTSGGKTYETGFHYLLEAHK",
    "response#3": "CEKTGLESGGVAHGGALNAAQVAHLDEDAFKGGLHRPKFDSEGLHKPHTSGGKTYETGFHYLLEAHC"
  },
  {
    "reference": "MALQPAAGARDLNPRQVESNRALSERLASVFRLWGYDEVSPPRVERLDTLMAGGAIASEDVVRLVADEPLGLRPEMTASIARAACTRLASRPRPLRLWASGTVFQSRAADEGGQCIEENLQCGVELFGVAPIEAEMELLSLLMAAVERLDFQAQHQPRLLMGHTGLMDLLLSPVPPVLRDAVRAALIQYDRLGLETIELEEGLRATLLSLLDCRGTPNEVLERLSSCFGAQALFDDLHRLCRQLQGPAAAQGVRLQLDPTFQPRFELYTGLVFQLVCDTHSAPVVVARGGRYDDLVRRCGAQPGQDFGAGFSLAIDPIRELLSDSSSNPTVAPQLMVAFSERSTLEAALERQRWWHQQGRSAVIELHPFSTRSLAEQQATDQGGFQLDWIDP",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be designed to localize specifically to cytoplasm in order to perform its role in histidine biosynthetic process more effectively.2. For general function, the protein need meet that May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine.; Required for the first step of histidine biosynthesis3. The protein design should consider the endogenous proteins and enzymes present in the Amino-acid biosynthesis, L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9 pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "KPMDVTTYVEHGVADAGIAGKDVMLEEERDVYEVLDLKISKCHLAVAGLPGADWNGVAPRIATKYPNVASSYFREQGEQVEIIKLNGSIELAPLIGLADRIVDIVSTGRTLKENGLVESEHICDITSRFIVNPVSYRMKDDVIDEMAARLY",
    "response#2": "LVYSSKESVAESLKTGTGVYQSRKRGLWYKGASSGAVQHLIHIDVDCDEDCLRFVVYQTGKGFCHLDTLHCA",
    "response#3": "LVYSSKESVAESLKTGTGVYQSRKRGLWYKGASSGAVQHLIHIDVDCDEDCLRFVVYQTGKGFCHLDTLHCG"
  },
  {
    "reference": "MSQLFYNNETISRIIKSQFDMALSHYGDIKYAYMVLNKKKPTEILIISNHHDEWREIYQANNYQHIDPVVIAALNKITPFPWDEDLLVSTQLKMSKIFNLSREHNITNGYTFVLHDHSNNLVMLSIMIDESNVSNIDDVIESNKDKLQMTLMTIHAETISLYREMIRNKEDERSNDKDIFSQRENEILYWASMGKTYQEIALILDIKTGTVKFHIGNVVKKLGVLNAKHAIRLGIELQLIRPVQS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design should aim to mimic or enhance the function of natural proteins involved in quorum sensing, potentially with improved properties such as stability, specificity or selectivity.2. The protein contains novel HTH luxR-type domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "GIAHEMRNPLSGLLTSIDVIQSVLPNPKEGKKEQYTLSDEDVTLLREVSSDAMKIIHSGNETIDLLLTSIDENRVSRSTFKKHSAQSVVESAIESFSYKRSTDRFAISLDVRSEFDFLGSDTLLKYVMYNLFKNAFHHRSSEDFHIHVTMYSDEFANQIVVTDNGSGIAPEVLQSIFQDFYTTGKSGNYGLGLPFCKKVMRSFGGDIRCQSEVGEWSQFTMTFPTIN",
    "response#2": "GIAHEMRNPLSGLLTSIDVIQSVLPNPKEGKKEQYTLSDEDVTLLREVSSDAMKIIHSGNETIDLLLTSIDENRVSRSTFKKHSAQSVVESAIESFSYKRSTDRFAISLDVRSEFDFLGSDTLLKYVMYNLFKNAFHHRSSEDFHIHVTMYSDEFANQIVVTDNGSGIAPEVLQSIFQDFYTTGKSGNYGLGLPFCKKVMRSFGGDIRCQSEVGEWSQFTMTFPTIR",
    "response#3": "GIAHEMRNPLSGLLTSIDVIQSVLPNPKEGKKEQYTLSDEDVTLLREVSSDAMKIIHSGNETIDLLLTSIDENRVSRSTFKKHSAQSVVESAIESFSYKRSTDRFAISLDVRSEFDFLGSDTLLKYVMYNLFKNAFHHRSSEDFHIHVTMYSDEFANQIVVTDNGSGIAPEVLQSIFQDFYTTGKSGNYGLGLPFCKKVMRSFGGDIRCQSEVGEWSQFTMTFPTIR"
  },
  {
    "reference": "MKARGMKRNYQXLWRWGIMXFWMIIMCKATQNLWVTVYYGVPVWRDAETTLFCASDAKAYDKEVHNVWATHACVPTNPDPXEIXLXNVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLDCTXPSSNSVXNSSXXSSGNWSEEMRREMRNCSYNITTELRDKRKNVYSLFYKLDIVPIDSNESSNXNXSRQYRLINCNTSTTTQACPKVSFEPIPIHYCTPAGFAILKCSEKNFTGKGPCXNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVMIRSENITNNAKIIIVQLKEPVNITCIRPSNNTRTSMRIGPGQTFYATGEVTGDIRXAXCNVXRAQWNNTLYKISTQLRKYFKNNTTIIFKNSSGGDLEVTTHSFNCGGEFFYCNTTDLFNSTWDANSTATNSTQNNETITLQCRIKQIINMWQRVGQAMYAPPIXGIIRCESNITGLLLXRDGGRNNSDNETFRPIGGDMRDNWRSELYKYKVVQVEPIGIAPXXARRRVVXREKRAVGLGAAFLGFLGAAGSTMGAASMTLTVLARQLLSGIVQQQSNLLQAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNSSWSNKSYXEIWDNMTWMQWDKEVNNYTAIXYNLIEEAQNQQEKNEQDLLALDXWASLWNWFSITNWLWYIRIFIMIVGGLIGLRIVFAVLXIINRVRQGYSPLSLQTPTLHPEGADRPGGIEEEGGEQDRNRSIRLVSGFLALAWDDLRS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should contain at least one YXXL motif; contains endocytosis signal motif to achieve its intended function.2. The protein should be designed to localize specifically to host cell endosome membrane, plasma membrane, host cell plasma membrane in order to perform its role in positive regulation of receptor clustering, actin filament reorganization, viral protein processing, fusion of virus membrane with host endosome membrane more effectively.3. Include a signal peptide as an essential component of the protein.4. The designed protein must have at least one Human immunodeficiency virus 1 envelope glycoprotein Gp120 domain.5. The protein must exhibit the following characteristics: Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane. The designed protein sequence is ",
    "response#1": "MRGEIKNCSYNITTEVRDKKRKEYSLFYKLDVVEMDEDNNKTQYRLINCNTSAITQACPKVSFEPIPIHYCAPAGYAILKCNDKNFTGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEEKIMIRSENITNNAKNILVQFTEPVEINCTRPNNNTRQGVHIGPGQAFYAIGEIIGDIRQAHCNVSGTKWNETLQNVTEQLKTLFNKEVRFANSSGGDLEITTHSFNCKGEFFYCDTSGLFDSQANRTNDTITLQCRIKQIINMWQRVGQAMYAPPIKGVINCTSHITGLILTRDGGNDTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVGREKLVLPGELAKHAVSEGTKAVTNNVTMDNNYKEEMQNCSFNVTTELRDKKQKAYSLFYKLDIVPLEGNTSSDSTSREYRLINCNTSAITQACPKVSFDPIPIHYCAPAGYAILKCNNKTFNGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIIRSENLTNNVKTIIVHLNESVAITCTRPNNNTRQSVRIGPGQVFYTNDIIGDIRQAYCNISKEKWNQTLQQVTEKLEELFPNKTIKFEPAAGGDLEITTHSFNCKGEFFYCNTSKLFNSPNDTESQPNYTIIPCRIKQIINMWQAVGRAMYAPPIAGNITCASNITGLLLSRDGGNTNTNGTSNTETFRPGGGDMRDNWRSELYKYKVVEIKPLGIAPTGAKRRVVEREKRXLGAGAVFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLKAIEAQQHLLQLTVWGIKQLQARLLAVEKYLKDQKFLGLWGCSGKVICTTAVPWNSTWSNKSYNDIWNNMTWTEWEREISNYTEQIYSLLTESQNQQDKNEKDLLELDKWTNLWTWFGITNWLWYIRIFIMIIGGLIGLRIIFAVLSIVNRVRQGYSPLSFQIP",
    "response#2": "MRGEIKNCSYNITTEVRDKKRKEYSLFYKLDVVEMDEDNNKTQYRLINCNTSAITQACPKVSFEPIPIHYCAPAGYAILKCNDKNFTGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEEKIMIRSENITNNAKNILVQFTEPVEINCTRPNNNTRQGVHIGPGQAFYAIGEIIGDIRQAHCNVSGTKWNETLQNVTEQLKTLFNKEVRFANSSGGDLEITTHSFNCKGEFFYCDTSGLFDSQANRTNDTITLQCRIKQIINMWQRVGQAMYAPPIKGVINCTSHITGLILTRDGGNDTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVGREKRXXXXXXXYTETKNCSFKTNPNLKDKSQRAYASFYKLDIVPLEENSTTYRLISCNTSSITQACPKVSFEPIPIHLCAPAGFAILKCRNDTFNGTGPCDNVSTVQCTHGIRPVVSTQLLLNGSLAKKEIVIRSENFTDNAKTIIVQLNKSVNITCVRPGNNTRKSIHIGPGRAFYATGEIIGNIRQAHCNISRQAWEHTLNETAKKLGEQLKITTIIFQPSSGGDLEIETHSFNCGGEFFYCNTTELFNSVWNNSGRVTGTGNSTNITLPCRIKQFINMWQEVGKAMYAPPISGQIKCSSNITGLLLTRDGGPNKTENDTFRPTGGDMRNNWRSELYKYKVVQIEPLGIAPTTAKRRVVQREKRXXXXXXX",
    "response#3": "MRGEIKNCSYNITTEVRDKKRKEYSLFYKLDVVEMDEDNNKTQYRLINCNTSAITQACPKVSFEPIPIHYCAPAGYAILKCNDKNFTGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEEKIMIRSENITNNAKNILVQFTEPVEINCTRPNNNTRQGVHIGPGQAFYAIGEIIGDIRQAHCNVSGTKWNETLQNVTEQLKTLFNKEVRFANSSGGDLEITTHSFNCKGEFFYCDTSGLFDSQANRTNDTITLQCRIKQIINMWQRVGQAMYAPPIKGVINCTSHITGLILTRDGGNDTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRARRRVVGREKRAVLGAMFLGFLGVAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGIWGCSGKLICTTNVPWNSTWSNKTKQEIWGNMTWMQWEREIDNYTETIYQLLEESQNQQERNEQDLLALDKWDSLWSWFSITNWLWYIKIFIMIIGGLIGLRIIFAVLSIVNRVRQGYSPLSLQTLIPNPSGPDRPGGIEEEGGEQDRDRSIRLVNGFLALVWDDLRSLCLFSYHRLRDLL"
  },
  {
    "reference": "MLKEIRPAIFVLLALTLITGLLYPLAMTGLAVTIFPAQAAGSLITRNGQVIGSALIGQEFKDDRYFHGRPSATSTADPNDSTKTVPAPYNAANSSGSNLGPTSKALADRVKEDVDKLKAENPAQPVPVDLVTTSASGLDPHVSPEAAMFQVPRVAKARGVPEDRVRALVVKNTEGRLIGLLGEPRVNVLALNLALDAATATK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should be optimized for plasma membrane-specific folding and stability, while maintaining P-type potassium transmembrane transporter activity.2. The protein must exhibit the following characteristics: Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm. The designed protein sequence is ",
    "response#1": "VLAMSGRAIEAAGDVDVLLLDKTGTITLGNRKASEFLPVDGVTEQELADAAQLSSIADETAEGRSIVVLAKERFDIRGRDFAEMHAEFVPFTATTRMSGIDYQENTIRKGAADAVRAYVTANGGTYPKECDAIVSKVAGAGGTPLVVVRNNKVLGVIYLKDIVKNGVKERFLDLRKMGIKTIMITGDNPMTAAAIAAEAGVDDFLAEATPEAKLELIREYQREGHLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEAGNMVDLDSSPTKLIDIVRIGKG",
    "response#2": "VLAMSGRAIEAAGDVDVLLLDKTGTITLGNRKASEFLPVDGVTEQELADAAQLSSIADETAEGRSIVVLAKERFDIRGRDFAEMHAEFVPFTATTRMSGIDYQENTIRKGAADAVRAYVTANGGTYPKECDAIVSKVAGAGGTPLVVVRNNKVLGVIYLKDIVKNGVKERFLDLRKMGIKTIMITGDNPMTAAAIAAEAGVDDFLAEATPEAKLELIREYQREGHLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEAGNMVDLDSSPTKLIDIVRIGKE",
    "response#3": "VLAMSGRAIEAAGDVDVLLLDKTGTITLGNRKASEFLPVDGVTEQELADAAQLSSIADETAEGRSIVVLAKERFDIRGRDFAEMHAEFVPFTATTRMSGIDYQENTIRKGAADAVRAYVTANGGTYPKECDAIVSKVAGAGGTPLVVVRNNKVLGVIYLKDIVKNGVKERFLDLRKMGIKTIMITGDNPMTAAAIAAEAGVDDFLAEATPEAKLELIREYQREGHLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEAGNMVDLDSSPTKLIDIVRIGKR"
  },
  {
    "reference": "MATKRICIIGGGASGITAAKACIEEGHDYVIYEMYHTFGGLWRYRDEDVNGLASVAAATIINSSKEMSAFSDFPPREDYPNYMHNTRMIEYIEDYAKRVGCQEKMFFQHRVLSCEKAASFDEDGCWVVTVEDMQTKRSFKETFNGIMVCTGHHVTPSWATFPNQDKFKGQIIHTHSYKKPGEFVGKRVVVVGIGNSGGDVAVELSMVNPKVHLSTRRGAWVVHRVGMHGKPFDTLFLRRWINVLFNCIPYRMVCSTAENFINFHFDHSQYRIKPQHRIFEQHITVNDALPNRILSGTIELKNDIESFTETGVIFKGDHEETPCDVVIMATGYKVDFPFIDQSLVPVVDNKVDLYKYVFAPNLQPHPETLAFIALAQPIGALFPIAEMHSRWFALVMSGKVQLPSQKAMLKDIEKKHKYIQSRYYSGPRNTLQVDWINYMDELAELIGCKPNVSKYLFKDPKLWWQLVAGPCFPYQYRLTGPHKWEGARNCLLEANRRIKAPLFTRELTDKNNEFIKSAADTRNGYKKIQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must be engineered to have a high affinity for FAD in order to increase its catalytic activity.2. The ideal protein would be one that plays a critical role in lipid metabolic process through hypotaurine dehydrogenase activity, NADP binding, N,N-dimethylaniline monooxygenase activity, NAD(P)H oxidase H2O2-forming activity within endoplasmic reticulum membrane.3. The desired protein should be able to perform H(+) + hexan-3-one + NADPH + O2 = ethyl butanoate + H2O +   NADP(+) The designed protein sequence is ",
    "response#1": "MGDAG",
    "response#2": "MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAIKS",
    "response#3": "MXKXDXWX"
  },
  {
    "reference": "MSLLTPDSGLLFWMIVSFGIVFVILSKYGFPVIVKAIEQRKAYIDNSLETARQANERLAHIQAEGEKMLAEAKEKQNAVLKEAFAEKERIIEEARKKAVSEAHLQIEEATRRIREEKEKAIREVRSEIADLSIAIAEKVMKEKIGRDKEQQQMIDRLLDEVSFSKS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must be optimized for proton-transporting ATP synthase activity, rotational mechanism.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. For general function, the protein need meet that Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0). The designed protein sequence is ",
    "response#1": "GVPTTAP",
    "response#2": "GFASRFKF",
    "response#3": "LCFIC"
  },
  {
    "reference": "MDSKTIEIIKSTVPVLEQHGEKITTVFYQQMFEAHPELLNIFNHANQKQGRQQKALASTVYAAAQYIDNLEAIVPVVTQIAHKHRSLGIKPEHYPIVGEHLLGAIKTVLGQAATDEILDAWEQAYNTIADVFISVEAQMYQDAKNQHGGWDGFKSFVVDRKVVESEVITSFYLKPEDKQMLPSFEAGQYISVKLEIEEEKFTHIRQYSLSDAAGKDYYRISVKRETGNETPDGIVSNYLHEKMNEGDTIQVSAPAGDFYLDTGKDTPLVLLSGGVGLTPMMSMLNTVVDLQPERPVTFIHATGNGRVHALRDEVKRVAEKGNVNVVFFYDSPTEEDRETQAFDIEGFVTKEWLRENVPHNDTSFYFCGPVPFMKSVNASLKSLGVTDKNIHFEFFGPMGSLE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The presence of heme b is necessary for the protein to function in the desired environment.2. The Charge relay system active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. The designed protein have FAD-binding FR-type, Globin family profile domains with specific sequence motifs that are crucial for its activity.4. The protein must exhibit the following characteristics: Therefore, plays a central role in the inducible response to nitrosative stress..5. A protein with nitric oxide dioxygenase activity, oxygen carrier activity functions is required to enable response to toxic substance, response to nitrosative stress.6. The designed protein should have a binding site that is specific to the target heme b, FAD ligand. The designed protein sequence is ",
    "response#1": "MLSQNTIKVIKSTVPVLEVHGEAITRHFYETMFEHHTELLNIFNHANQKQGRQQAALANMVYTAAQHIDNLGSILPAVRQVAHKHRSLGIVPEQYAIVGTYLLQAIKDVLGDAATDEIITAWSEAYNVIADAFIGVEKGMY",
    "response#2": "MLSQQTIDVIQSTVPVLEVHGEAITRHFYQTLFTAHPELLNIFNHANQKQGRQQAALANMVYTAALHIDNLAAILPAVRQVAHKHRSLGIVPEQYAIVGTYLLQAIGDVLGEAATDEILAAWGDAYQVIADAFIGVEE",
    "response#3": "MLSQQHIDTVKATIPLLASAGTAITEHFYNRMFTHNPELKNVFNMTHQKTGRQPAALFNAIAAYATYIDNLEALTSAVMRIAHKHTSFNIQPEQYDIVGHHLIETLRELAPDAFTKEVEEAWTAAYVQLAEIFIE"
  },
  {
    "reference": "MTSRWTTAVLDTDQPGGWAVARSPEGFLFDDNGVLFPREWLKRQDLSILAEHGIGHLDGEPVYLLELHSTSEVPGCNWKGLRAFMLDGDHTIYKVLGYAAQIGTWAREHRFCGNCGQAMTQVPRERAMYCQPCDLRSYPRISPSMIVLVTRGDEVLLARSPRFVTGVYSTLAGFAEPGESAEDCLIREVREEVQIEVKNIQYMGSQCWPFPHSMMLGFHAEYAGGEIVCQEDEIEDAQWFNVHDLPPLPASKSIARYLIDVYVARRLGHAEPVLPG",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must have at least one Nudix hydrolase domain.2. The designed protein must be optimized for NAD+ diphosphatase activity, manganese ion binding, NADH pyrophosphatase activity, magnesium ion binding, RNA NAD-cap (NMN-forming) hydrolase activity.3. The protein must bind to Zn(2+) in order to perform its enzymatic function.4. The designed protein should contain at least one Nudix box motif to achieve its intended function.5. The desired protein should be able to perform H2O + NADH = AMP + 2 H(+) + reduced beta-nicotinamide D-   ribonucleotide6. For general function, the protein need meet that mRNA decapping enzyme that specifically removes the nicotinamide adenine dinucleotide (NAD) cap from a subset of mRNAs by hydrolyzing the diphosphate linkage to produce nicotinamide mononucleotide (NMN) and 5' monophosphate mRNA; Has preference for mRNAs with a 5'-end purine; The NAD-cap is present at the 5'-end of some mRNAs and stabilizes RNA against 5'-processing7. The protein should be able to bind substrate, Zn(2+), a divalent metal cation ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "MIDWRHACHALLYAPDPRKLFGRIPMRFAVLMQMRFDGRLGFPGGFVDAQDSCLEDGLNRELREELGEAMSAFRVERSDYRSSHIAARPRVVAHFYAKRLTLEQLQAVEARAPQAKDHGLEVLGLVRVPLYVLRDGEGGLPAFLENSFIGAAREQLLARLVAVAT",
    "response#2": "MIISPSMIVLITRGDEILLARSPRFVTGVYSTLAGFAEPGESAEDCLIREVREEVSIEVRNIQYVGSQCWPFPHSMMLGFHAEYAGGEIIPQEDEIEDAQWFSVHALPPLPASRSIARYLIDLYLKDLVENSSAGFLEAGETLEDCVAREVHEETTVYILFSNLSPLVTLGMGTERSQPPEVKLCRLYHKDVERYMSQTEEVTLIFLGVELQFNKNFMAARNEKVLEEEEDDGLVAWFALSIDPTSAEKFKEKHEDCYFLHPPMPALLQLTEKEAGVVAQARSVLADV",
    "response#3": "MIIYRSNVGIILLNSKNEVFWGKRIRQDSWQFPQGGIKPGESPEQAMYRELTEEVGLRPWHVQILGRTRDWLRYDVPAQWIKRDWRGNYRGQKQIWYLLRLLGSDCDVSLHTSGRPEFDAWRWNQYWVELESVVEFLGEDGLDYVKKDTHVFAACITSDGYPLIGARRTSFAFQAILSQQNSDSIFRVSTKLLRFMYYNELREIFRRLRKGSINNIDPHFEELILLGGKLDKKESIKDCLRRELKEESDERITVKEFGNVILKLTTQDKLFNKVYIGYCMSCFINQSLEDLSHTSIYNVEIRKIKSLNDCINDDKYEYLSYIYNMG"
  },
  {
    "reference": "MKLSQIIAPILTAKMIGNPNVEINGVEVDSRKVKAGDLFICLLGFTVDGHDFAQKAVEQGAVAILCERPLDLQVPQVIVKDARFAMAMIADLFYQHPSQKLKVIGVTGTNGKTTTTHLIEKILADQGHLSGLIGTIKMKIGNEIVEAKNTTPDALLLQQSFAKMVKVGSDYAIMEVSSHALDMGRVRGVNYHIGVFTNLTQDHLDYHLTMEKYRDAKGLLFSQLGNTFHSLLEENKYAVLNADDQASEYFQQITAAQVITYGIDHDADVKALDIHIDAQGTSFAVETFRGNIDIQLKMIGKFSVYNALAAMTVGLIEGISLDQIKQSLEQISGVDGRFEPVNEGQDYTVLVDYAHTPDSLENILKTVKEFAKGKIYCVFGAGGDRDKTKRPIMGEIAVKYSDIAVVTSDNPRSENPEQIIHDILVGIEKTGVDTNKYIVIIDRKEAIEYAVSQAKKDDVILIAGKGHETYQILKNKTIHFDDREIARDAIRSKRS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan..2. The ideal protein would be one that plays a critical role in cell wall organization, regulation of cell shape through magnesium ion binding within cytoplasm.3. A protein should be able to manipulate the activity of enzymes in the Cell wall biogenesis pathway, either by activating or inhibiting their function.4. The ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGVAAVIAEANGQAEDGAIVEMHGVPIIYLSQLNQRLSALAGRFYGHPSDRLQMVGVTGTNGKTTTTHLIEFLLQEAGTRAGLYGTINYRIGDRSIPSIRTTPDAVDLQRLLAELVAAGCEYAAMEVSSHALQQGRVDGCSFDVAVFTNLTQDHLDYHVTMENYLAAKARLFSRLEAGTRSRGFAVVNLDDPAGRRLAADCAVPVITYGIDRGADVRAENVRTDAGGTSFTLVSPQGEFAIKSPLVGRYNVYNVLAAAAACYALGIPAETIKSGLEAVPQVPGRIEAFRGPAAAPLIVVDYAHTPEALRQILSAVRAHARGRLYCVFGCGGDRDRGKRPLMGAAAASLADHLIVTDDNPRSESPQAIVEEILAGIPDAMRDAVRVIHDRAEAVRCAVEEAGAADVVVVAGKGHETYQILGDRTIHFDDRETARQAIAERKQG",
    "response#2": "MTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGVAAVIAEANGQAEDGAIVEMHGVPIIYLSQLNQRLSALAGRFYGFPSRGLSLVGITGTNGKTTTTHVIHKIMSDHQVETAIIGTMYMKYKGKEIAVANTTPESLVLQKEYAHMVEAGVEAVTMEVSSHALELGRVHGTEFNIGVFTNLSQDHLDYHKTMEKYAQAKGLLFAQLGSSFSASNQNVAVLNLDDPYFSQLETMTAVPVLTYGMGEQADFRAVDVKIHENGADFTLQLGSEQYDIHLQMTGRFSVYNALAAAACGYALGVDVEKIVAGIEAFPGVPHRCEWVADKDGVAYINDSKATNVGATQAALAGLGPVTSGGIVLICGGQGKGQDFTPLAPAVKEWVSTLIIIGEDGPKLKEALAGGVMALSAETMEEAVKLAAEKSSPGDLVLLSPAGSV",
    "response#3": "MTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGVAAVIAEANGQAEDGAIVEMHGVPIIYLSQLNQRLSALAGRFYGHPSRELSLVGITGTNGKTTTTALVAAMFQTAGLKAIACGNIGYSVCDLVLSEQAIDWVIAEISSYQLESLPRLAPKIAVWTTFTPDHLERHGTLECYRETKAVLLQQAEYKVLNGDDPYLRQELSDRWPEVWWTSCNGRAALPHGIERGIYLEEGWVKVQEQPIVPVSSWKMPGHHNLQNLLLAVAVAGALGYAPEVIREGIAGFPGVPGRLEAVSEARGALCVVDYAHKPEALAHALEALRPFTRGRLVCVFGCGGDRDPGKRPIMGRIAAERADLVVVTDDNPRNEEPATIRAAILAAAPGAREIGDRAQAIREAVSGLRDGDVLLVAGKGHETYQIIGQKKIPFDDRIVARKAIAELVKKGCL"
  },
  {
    "reference": "MGIASILSNKNITINVDHVIDSDEAMLLAIGYRQELRREFSLWSIFAVSFSVLGLLPSIAACFDYQQLVVGMSPLPWLIAMIFITSVAYSMAEIASAFPCSAGTPYAVSQLAPKKYASFLTWFTCWTNWSCQITAAPSVSYSCACMMLALHSFTDPSFVASNAQIFGLTTGIQVLCAFMACFPTKWVARFSSAGTTCNIVFLVVVFIMILGGNKRDQIKEGISKFNSNSTAWGLDNQAEWPTGLSFLISFMGVIWAMSGYDSPFHLAEKCSNAAVAAPRAIVLTSTVGGLIGFMFMIAIAYTLVDLNQISADPESLGQPFVTYLTQIMDKNLVIGATALTIISSFFMAQNCLLASSRVTYAYARDGLFPLSGIWKKVSPKTQTPINAVIMNFIVEELLLLLIFGGDVSIGSIFSIGALAGFISFTMPTLLKITYARKTFQPGPWNLGKWSEPIGWVSVAFVGLMVPILCFPTVKGADLTPTEMNWTCLVYFGLILLTTIWFVVYARRWYVGPRTNISEEDIVYGEKTEDEGDEIPDVIDGQKVSISSTEKRYQ",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The designed protein localizes to the membrane. The designed protein sequence is ",
    "response#1": "KGALLVCLAATMWGFDGIALTPRLFSLHVPFVVFILHLLPLILMSILFGKEEVKNIKKLQKNDLFFFFCVALFGGCLGTLCIVKALFLVNFKHLTVVTLLQKLQPIFAIILARLLLKEKLKRAYLFWGFLALLGGYLLLV",
    "response#2": "KGALLVCLAATMWGFDGIALTPRLFSLHVPFVVFILHLLPLILMSILFGKEEVKNIKKLQKNDLFFFFCVALFGGCLGTLCIVKALFLVNFKHLTVVTLLQKLQPIFAIILARLLLKEKLKRAYLFWGFLALLGGYLLY",
    "response#3": "KGALLVCLAATMWGFDGIALTPRLFSLHVPFVVFILHLLPLILMSILFGKEEVKNIKKLQKNDLFFFFCVALFGGCLGTLCIVKALFLVNFKHLTVVTLLQKLQPIFAIILARLLLKEKLKRAYLFWGFLALLGGYLLF"
  },
  {
    "reference": "MADSSVNLSQGLLTFRDVTVDFSQEEWECLDSAQRALYIEVMLENYSNLVYVENYCICDTVCQHVKTEKESCNELGEMLHEPSNCALYNRSDTTEASNNYRCCKDQDASLDSSNPDRLKSTHTGKERCESKDCAKSSSLCSSIAQDQRTNSTNKEQRQEKYDDHFISTHSLMQQAIYIGENPHQCMKYGKCFSSASSLGVQQRTDTGNKPYKCNICDKSFTECSSLKEHRKTHQRLRAGTNPYKCNDCGKSFSYLSALQSHHKRHTGEKRYKCKECGKSYAYRTGLKRHQKIHTAEECYSCQYCGKVFHQLSHFKSHFTLHTGEKPYKCNECHRSFPHYVFFRRHKKNHSLQKSHKCKECGKSFFILSHLKTHYRIHTGEKPYKCTKCDKLFTQYSHLRRHQRIYTGKKLYRCEVCDKWFTLSSSLSRHQKIHTEAKTYKCKDCDIFFNHYSSLRRHQKVHTGERHYTCKQCGKSFTRGSTLRVHQRIHTGEKPYKCSECDKSFTQASQLRTHQRVHTGEKPYVCKECGKSLTTCAILRAHQKIHTGEKPYKCMECDRSYIQYSHLKRHQKVHTGEKHKIVNNVT",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The presence of KRAB domains is critical for the interaction of the designed protein with its intended targets.2. The ideal protein would be one that plays a critical role in regulation of transcription by RNA polymerase II through RNA polymerase II cis-regulatory region sequence-specific DNA binding, DNA-binding transcription factor activity, RNA polymerase II-specific, metal ion binding within nucleus.3. The protein must exhibit the following characteristics: May be involved in transcriptional regulation.. The designed protein sequence is ",
    "response#1": "MRDEVAEKEKADINVTLVFQGYENTPIMVCVDGIVFSKPDLVTCLEQRKKPWSMKHPGLTQHNC",
    "response#2": "MRDEVAEKEKADINVTLVFQGYENTPIMVCVDGIVFSKPDLVTCLEQRKKPWSMKHPGLTQHNC",
    "response#3": "MTFKDVAVEFTQEEWMMLDSAQRSLYRDVMLENYRNLTSVEYQLYRLTVISPLDQEEIRNMKKRIPQAICL"
  },
  {
    "reference": "MSTADGAQTKYIFVTGGVVSALGKGIVAASLGRLLKARGLRVQAQKFDPYLNVDAGTMSPFQHGEVFVTEDGAETDLDIGHYERFIDENMSRNASHSAGAIWEAVLRKERKGEFLGATVQVIPHITNEIKARIKRAAKAAPADVVISEIGGTVGDIESLPFLEAIRQFRREAGPENVLYLHCTLVPYIEAAGELKTKPTQHSVNELRRIGIHPDVIVCRSHEELSQDIKDKIALFADVEVRSVVASPDVADVYLVPAVLREEGLDALVCEKLGIDAAPAELGEWQDLIDRIGEPNDGRQREPVTIALVGKYVKLHDAYLSVHEALKHAGLHQGSQVRVQWVDAENMSYEETAELLEGVDGVLVPGGFGSRGWEGKILACRVARERGVPYLGICLGMHVAVSEFARNVCGLDGANSTEMDPETPFPVIDLLPEQKEIEDLGGTMRLGAQAVEVADGTRTRDAYGGAVIHERHRHRYEVNNHYRPALVEAGLVVSGTFQEGRLVEIVELPDHPWFVASQFHPEFKSRPTRPAPLFREFVGAALERARSRSGVELRAAG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be optimized for activity in CTP from UDP: step 2/2, CTP biosynthesis via de novo pathway, Pyrimidine metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The designed protein should contain one or more Glutamine amidotransferase, CTP synthase N-terminal domains that are essential for its function.3. The designed protein must possess CTP synthase activity, ATP binding, metal ion binding to support 'de novo' CTP biosynthetic process, glutamine metabolic process under certain conditions.4. The protein should be able to bind UTP, Mg(2+), ATP, L-glutamine ligand in a variety of conditions5. The protein's Nucleophile; for glutamine hydrolysis, Nucleophile, active site must be carefully engineered to ensure that the amino acids involved in H2O + L-glutamine = L-glutamate + NH4(+) are properly positioned for optimal catalysis.6. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. The designed protein sequence is ",
    "response#1": "CVTGGVVSSLGKGIAAASLGAILEARGLKVSMVKLDPYINVDPGTMSPFQHGEVFVTTDGTETDLDLGHYERFVRTTTSRNSNFTTGRIYERVIAKERRGDYLGATVQVIPHITDEIKHCIRLGAGDADVCMVEIGGTVGDIESLPFLEAIRQLGVELGRNNVIYMHLTLIPYIPSSGEIKTKPTQHSVKELRSIGIQPDILLCRAHHPLPEEQRRKIALFTNVEERAVISAVDADDLYKIPLLLHEEGLDEIVVDKLRLDVPEPDLSDWQTMVDRVRSPRPETVTIAFVGKYLDLKESYKSLTEALIHAGAHINAKINIHWCDSERIEDAGAYEVIQTCDAVLVAGGFGHRGVEGKLKAIEYARTNNIPYLGICLGMQLAVIEYARNVLGIEDANSVEFDPEAKNPLIYLIDEFLDQSGNKQVRTHESPMGGTMRLGEYPFTPKKGSKLQKAYGDKETYYERHRHRYEANPKYKEALEKAGMIISGESHGLIEAVELKDHKWFVGVQFHPEFTSHLETPNPIILEFIRAAHAQHRASDSYLSVIKALQHSSFKVNRKLVIDWIESAELDEETAGTDPEAHXAAWELLKAADGILVPGGFGIRGIEGKIKAAEYARESGTPYLGVCLGLQVATIEFCRNVLGLERANSTEFXEDTPNPAVVFMPEISKTHMGGTMRLGTKPTPFLVDDCKMRRLYDGVNHVDERHRHRYEVNPDLIGQIESAGLKYVGKDETGERCXIMELEGHPYFVGTQYHPEFKSRPDKPSPPYLGLLHDAYLSVAEALSHGGIANDARVDIKWVHSEDLEEDGIDLEEIFSGVDGLLIPGGFGDRGIEGKIKAIRYAREKKIPLFGICLGMQCAVIEFARSVCGLQGAHSTEFDPNTPHPVIDLMPEQKDIEKKGGTMRLGHYPCRITPGTRTMEAYGEEMIEERHRHRYEFNNEYREVLEKAGMVISGLSPDGRLVEIIELKDHPWFVGTQFHPEFKSRPNRPHPLFRDFI",
    "response#2": "CVTGGVVSSLGKGIAAASLGAILEARGLKVSMVKLDPYINVDPGTMSPFQHGEVFVTTDGTETDLDLGHYERFVRTTTSRNSNFTTGRIYERVIAKERRGDYLGATVQVIPHITDEIKHCIRLGAGDADVCMVEIGGTVGDIESLPFLEAIRQLGVELGRNNVIYMHLTLIPYIPSSGEIKTKPTQHSVKELRSIGIQPDILLCRAHHPLPEEQRRKIALFTNVEERAVISAVDADDLYKIPLLLHEEGLDEIVVDKLRLECRAPDLSHWEELVHRIKSPSRSLSDSYISVIEAIKHAAYSLGHRPIIDWINSEKFEKGSVKENSELKNYDGIIVPGGFGTRGVEGKIKVIEYARKNKIPFLGLCYGMQMAAIEFVRNVCGKRDAHTTEINQNTKNPVIHILPEQLENMEKKNYGGSMRLGAYNCELKRGTKAYAAYKQPLISERHRHRYEFNNDYKEMMEKKGMIFSGINPEKNLVEILELKSHPFFMATQFHPEFKSKPLNPHPLFLEFIRAAAAS",
    "response#3": "CVTGGVVSSLGKGIAAASLGAILEARGLKVSMVKLDPYINVDPGTMSPFQHGEVFVTTDGTETDLDLGHYERFVRTTTSRNSNFTTGRIYERVIAKERRGDYLGATVQVIPHITDEIKHCIRLGAGDADVCMVEIGGTVGDIESLPFLEAIRQLGVELGRNNVIYMHLTLIPYIPSSGEIKTKPTQHSVKELRSIGIQPDILLCRAHHPLPEEQRRKIALFTNVEERAVISAVDADDLYKIPLLLHEEGLDEIVVDKLRLRDSYISLTEALIHAGLRTRTRVNIQFIEATDVEKHGTSCLEGADAILVPGGFGERGIDGKMAAIQYAREREIPYLGICLGMQLAVVEYARNVAGLEGANSTEFDRSTPHPVVALIAEWQDQKGTVEQRSEDSKLGGTMRLGAQEVRLSHGSLAHQIYGTDTIMERHRHRYEFNNRYLQKLMNAGLRFSGFSRDGLVEVVELPNHPFFIASQFHPEFRSTPRDGHPLFTGFIAASAKADGVRIGIIGKYFETGKFTLMDSYISVLEAVKHAAWFHKRKPEIYWLSARTYEKSPKFLNELKEFEGIIVPGGFGSRGIEGKIRAIEFCRKHKIPFLGLCLGMQLAVVEFARNVCKLRGAHSTEFNPKTAKPVIDVMPEQKALLKEKKYGGTMRLGEYKCKIKPRTLAFRAYKTSSVMERHRHRYELNNMFREALKIKGMVISGVNPEKDLVEIIELPQHPFFLASQFHPEFKSRPLSPHPLFREFLKAGL"
  },
  {
    "reference": "MLASKRILLIIGGGIAAYKVLDLIRRLRERGATVTPVLTRAGAEFVTPLSVSALAGRKVYSDLFDLTDEAEMGHIELSRSADLIVVAPATADLMARMAGGQADDLASTLLLATDTPVLIAPAMNVRMWQHPATRRNIAMLRGDGIGFVGPNEGDMACGEFGPGRMAEVSEIVAGIEAVLSAGPLQGRRVLVTSGPTHEPVDPVRYIANRSSGAQGAAIARALAALGAEVVFVTGPASVPPPGGVAVVCVETAREMLAAVEAALPVEAAVFAAAVADWHVAGASARKLKKDAGGLANLEFAENPDILARVAGMKKGRPRLVVGFAAETDNVVENATAKRARKGCDWILANDVRHETGIMGGAENAVTLITGEGVETWPRMGKDQVARQLAGRIAAALA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should consider the endogenous proteins and enzymes present in the coenzyme A biosynthesis pathway to avoid interference with native metabolic processes.2. The designed protein must be able to perform FMN binding under specific conditions. The designed protein sequence is ",
    "response#1": "KTVLLGVTGGIAAYKAAALASALVKQHAAVEVVMTENATQFVTPLTFEQLTGRRTMVNTFDRNFSHQVEHIALAERTDLVIVAPATANVCAKLAHGLADDMLTTTILACRCPKLIAPAMNTNMYENPVTQDNLALLRHYGWEVIEPASGRLACGAVGKGKLPEPEEILQYILRQVEEA",
    "response#2": "LSGKHVVVTAGGTQEPIDPVRYIANHSSGKMGYAFAQAAVEQGAQVTLITTVDLTPPPFTTVVHVKTAQEMQNALQCYYEAADLVIMAAAVADYRIAEPATQKMKKQQDSTALHLKLVENPDILAELGAQKTHQYLVGFAAETQQLLQHAQVKLEKKQADLLIANDVSQTDIGFGSADNAVTLIG",
    "response#3": "EVVLGVGAGIAAYKVCELLRLFTESGHRVQVVPTADALRFVGEPTWAALSGRPVATGVWDAVHEVPHVRIGQRADLVFVAPTTADLLAKAAHGLADDLLTNTLLTARCPVVFAPAMHTEMWEHPATQANVATLRSRGAIVLDPAVGRLTGADTGRGRLPDEVVLGVGAGIAAYKVCELLRLFTESGHRVQVVPTADALRFVGEPTWAALSGRPVATGVWDAVHEVPHVRIGQRADLVFVAPTTADLLAKAAHGLADDLLTNTLLTARCPVVFAPAMHTEMWEHPATQANVATLRSRGAIVLDPAVGRLTGADTGRGRLPDG"
  },
  {
    "reference": "MKQRTLLREVSLEGTALHTGDDVTLTIKPAPADHGIIFRRVDLHGSPEIRPHISQVSDLVRSTTITSGHAQIHTVEHVLSALHGYSIDNVVVEMNASEPPIMDGSARPYVNLILEGEPVEQDKEREFFDLDEPISVTRGNSSLIALPSDQLKISCTSADDRGIHTQHLSIALDPETYATQIAAARTFTIYEDIEELLKLGKIKGGSLDCAIVLKGDKIISKEPLRFPDEFVRHKILDIIGDILLVGKPIRAHIVAVRPGHAINADLTKALAEKLERKGRRRKARRSPAVLATETSLDIKRVLETLPHRYPFLMIDKVVEFVGDDELTAVKNVSFNEPYFIGHFPGEPVMPGVLQIEAMAQAAGIIMLRRVSNEGRTAFFMSCDKVKFRKAVRPGDQLVIRAKLTKSRGNKIGVAEATCTVDGEVVSTGELMFALLESEGRE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.2. The desired protein should be able to perform a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine +   H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate3. The protein design should take into account the physiological conditions of the lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 2/6, lipid IV(A) biosynthesis pathway.4. A protein that can perform 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity in cytoplasm to support fatty acid biosynthetic process. The designed protein sequence is ",
    "response#1": "MPIPSIEEAIEEIKKGKMIILVDDEDRENEGDLAIAAEKVTPEIINFMTKYGRGLICLTLTPDIVEKLDLPLMVSSNTSSFGTAFTISIEARRGVTTGISAADRARTILTAIADDTKPEDLARPGHIFPLRAREGGVLVRAGQTEGSVDLARLAGLKPAGIICEIMNEDGTMARLPQLEKFSKKHNIPICTIADLISYRLRHEKLVRKVAVEELPVKIETPHGKFLKVKVEAITHEKLPIEPLKNNLRYLRTKREKMGHHLENLKEGIEKIKPKKLYFNVESDILGHMYLKLEKKEEVIKLGEVKNEDVFKFLKELKEDKNVVLIYCNEEEIERLKLSFHVKVKEIEVKESPIGHETYMYIKTKKY",
    "response#2": "MPQHPLAGRVTSFGFHLIPPDKRQWLTEVYSRVATPLAEDITGWTVQDLLDPDQSVLAFCRQLAKTAMWSWPADPIYLSAHPNLVARHPDRVDLLAGFFGRLMSAKPVNLLAGCSLVLSRIVPLDHPAAARIAAANPDEPLGFYHFRHPETGQERLLEADPIYFRIFHDFFTGGPAQAMGLVHSQVIVRDGEEWYVLSDAEGLGKTTIALGLMQQGWRFIGDDRPVIRPDAKGTLFPLPYPLSVYPGSFGHFFGPPPGGTPHRWTEGGPRQRVYWDPALPAALPDPAPPPRLLILPRYVPRRQVNRITRLDPLQALARLAAETAWQLRFLDRLPPLLSVPAAVLATLADRLPVFELAFPPGPLEAQADLLAGLLGG",
    "response#3": "MFSPKSEVKESIHQNRSMNGPKFSSSKDIAQWLKQQEQVALVTHEKPDADSLGSELAMRAVLKSLGKKASLFNPNAVPPKYQFLPGAETLKNSLPKDCDTLIVLDCGSLGRTGPLAESLKANPKIIVIDHHQTNEQFGDINLVDSEAAATSQLVWEFIKALGIEIDKATATALYVGIKTDTGGFRYSNTSAKCLQIAARLIDHGASLTEISERLFTSNTLGKLKLLNRTLSNLQLESDGKVAWTYISSEMLLETNASEEELDGFINYARKVKGVNIAAFFKELPDGTVKVSLRSRNGIDVSKIAAELGGGGHINAAGCTFTSTLEETKELVVKKLNDALS"
  },
  {
    "reference": "MNGKRILLIVGGGIAAFKVPELIRRLRERGAAVTPVLTRAAQEFVTPLTVSSLAGEKAYTDLFDLTDEAEMGHIQLSRAADLVLVVPATADLMAKMAGGHADDMASTLLMATDTPVMIAPAMNVRMWEHPATQRNLAALQQDGITVLGPDQGDMACGEYGPGRMVEPMQIVEAVTAALTGGPLRGKRVLVTSGPTHEPIDPVRYIANRSSGAQGTAIASALAALGADVIFITGPADVPPPTGVTVVPVETAQQMLQAVQDALPVHVAVFAAAVADWRVASSSASKIKKQDGKLPVLEFAENPDILATISQMKTGRPDLVVGFAAETDDVIANATAKRARKQCDWIVANDVRPETGIMGGAENAVILIREDGVEEWPRLDKAEVAKRLAKRIADKVEEA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be optimized for phosphopantothenoylcysteine decarboxylase activity activity in order to improve pantothenate catabolic process. The designed protein sequence is ",
    "response#1": "RVLLVVGGGIAAYKSCELVRLIRKGGGDVTCVVTDGGQQFVTPMALAALSENQVFTSLWDLKNESEMGHIQLSREADLVVVCPATANLLAKMSAGIADDLATTMILATDKPVLAVPAMNVRMWEHEATQRNVEWLRQAGVSVLDPDEGTMACGEFGPGRLPDPEAIWAAIAEIVTAL",
    "response#2": "MNIVLGVGGGIAAYKACHVLRALREAGHAVRVVPTASALAFVGTATWEALSGQPVATDVFTRVDEVEHVMIGQRADLVLIAPATADLLARLRAGRADDLLTATVLATRAPVALAPAMHTEMWANAATADNVAVLRARGLHVMEPVSGRLTGADSGPGRLPEPEDIVAFAL",
    "response#3": "MNIVLGVGGGIAAYKACHVLRALREAGHAVRVVPTASALAFVGTATWEALSGQPVATDVFTRVDEVEHVMIGQRADLVLIAPATADLLARLRAGRADDLLTATVLATRAPVALAPAMHTEMWANAATADNVAVLRARGLHVMEPVSGRLTGADSGPGRLPEPEDIVAFAVAK"
  },
  {
    "reference": "MSTKIVETITLECETGNYHSFCPISCVSWLYQKIEDSFFLVVGTKTCGYFLQNALGVMIFAEPRYAMAELEEGDISAHLNDYEELKTLCIRIRKDRDPSVIIWIGTCTTEIIKMDLEGMAPKLEYEIGVPILVARANGLDYAFTQGEDTVLAVMAHRCPDQEFPIGESKETKKKLFPFPLLKENNLVEYANHPPLVIFGSLPSNLVSQLDTELRRQFIKVSGWLPAQRYADLPSLGDGVYVCGVNPFLSRTATTLIRRKKCELIVAPFPIGPDGTRAWIERICPVFGIEAQSLEEIEERIWESLKDYLDLVRGKSVFFMGDNLIEISIARFLIRCGMIVYEIGIPYLDKRYQAAELALLKKTCIRMCMPIPRIVEKPDNSNQIRRMRELKPDLAITGMAHANPLGARGIGTKWSVEFTFAQIHGFANARDVLELVTRPLRRNENLDNLDRTTLVRKNNK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be optimized for activity in Porphyrin-containing compound metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The designed protein should have a binding site that is specific to the target [4Fe-4S] cluster ligand.3. The protein must exhibit the following characteristics: The NB-protein (ChlN-ChlB) is the catalytic component of the complex..4. A protein with oxidoreductase activity, acting on iron-sulfur proteins as donors is needed to enhance photosynthesis, dark reaction in a specific context. The designed protein sequence is ",
    "response#1": "PHVGAMRIAASMHGVHYVLHAPQGDTYADLLFTMIERRGQRPPVTYTTFQARDLGGDTAELVKRHVQEAADRFQPDALLVGESCTAELIQDQPGALAQGMGLPMPVVTLELPAYSKKENWGASETFYQLMRGLLKQSVPPQPSHDVQAWKHEGRRPRVNLLGPSLLGFRCRDDVLEVQRLLSLHGIDVGVVAPLGAGVEDILRLPQADLNVCLYPEVAESSCSWLERNFGIPFSKTVPIGMGATHDFLVEVHALLGMTPPEAAEGYQRSRMPWYSESVDSTYLTGKRVFIFGDGTHAIAAARICSEELGFTVVGLGSYSREMARPVRAAAKKLGLEALICDDYLAVEAAMAEAAPELVLGTQMERHSAKRLGIPCAVISTPMHVQDVPARLSPQMGWEGANVIFDAWVHPQ",
    "response#2": "PHVGAMRIAASMHGVHYVLHAPQGDTYADLLFTMIERRGQRPPVTYTTFQARDLGGDTAELVKRHVQEAADRFQPDALLVGESCTAELIQDQPGALAQGMGLPMPVVTLELPAYSKKENWGASETFYQLMRGLLKQSVPPQPSHDVQAWKHEGRRPRVNLLGPSLLGFRCRDDVLEVQRLLSLHGIDVGVVAPLGAGVEDILRLPQADLNVCLYPEVAESSCSWLERNFGIPFSKTVPIGMGATHDFLVEVHALLGMTPPEAAEGYQRSRMPWYSESVDSTYLTGKRVFIFGDGTHAIAAARICSEELGFTVVGLGSYSREMARPVRAAAKKLGLEALICDDYLAVEAAMAEAAPELVLGTQMERHSAKRLGIPCAVISTPMHVQDVPARLSPQMGWEGANVIFDAWVHPDQTA",
    "response#3": "PHVGAMRVAAAMKDVHYVLHAPQGDTYADLLFTMIERRESRPPVTYTTFEARDLGGDTAALFQRTAQEAYERFKPKALLVGSSCTAELIQDDPGGLARGLGLPVPVIPLEFSAYQRKENFGASLTFYNLVRAFAKALATPRATRSAARPSCNLLGATALGFRHRDDIREVTLLLDRLGVGVNICAPLGASPDDLARLPEADFNIVLYPEVALEAAEWLKRTHRQPLVKTQPIGVGATHAFIEEVARLAGLDPRPLLGPAESRLEWYSRSVDSTYLTGKRVFIFGDATHAIAAARVASRELGFTVAGLGSYSREFAREMRAAAALYSLNALITDDYLEVEAEIERLQPELVLGTQMERHIAKRLGIPCAVISAPVHVQDFPARHSPQMVFEGANVIFDSWVHPQEGARILKEFVQAV"
  },
  {
    "reference": "MPYYTRDDNDVDDFDEFDPTPYSGGYDITVIYGRPIPPSDETCYPLSSGVDDDFEYERPEFTQIHEPSAYGDEALNTEYSSYSRPKPRPAFRPDSGGGGHVQGERPNPGYGSESGYGRKPESEYGSGYGGQTEVEYGRRPEQSYGSGYGGRTETESEYGSGGGGRTEVEYGRRPESGLGSGYGGRSESEYERKPSYGRSEEQEEGYRKPSYGRSEEQEEGYRKPSYGRSEEQEEGYRKPSYGRSEEEQEEGYRKPSYGRSEEQEEGSYRKPSYGRSDDQVESYIKPSYGRSEEQEEGSYRKPSYGRSEEQEEGSYRKQPSYGRGNDDDDDEQRRNRSGSGDDEEGSYGRKKYGGNDSDEDEEKKKHRHKHHHQKRRDEDDE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should exhibit Basic and acidic residues compositional bias.2. The protein should be optimized for plastid, cytosol-specific folding and stability, while maintaining mRNA binding, phosphatidic acid binding. The designed protein sequence is ",
    "response#1": "NVLNRFFLMNRSDRNFEYGIQRDQIGKDTLNHRTRMKYMINQHLSNLKKSQKRWFDPLILISRTERSTNRDPDAYRYKWSNGSNNFQEHLDHFVSERKSRFRVVFDRLRINQYSIDWSEVIDKKGLSKPFRFFLSKSLLFLSKSLLFLSKFLFFLSNSLPFFFVSFGNIPIHRSEIYIYELKGPNDQLCNQLLESIGLQIVHLKKLKPFLLDDHDTSQKSKFLINGGTISPFLFNKIPKWMIYSFHTRNNRRKR",
    "response#2": "LIGATSLAFVICNMDKVNLSIAIIPMSHQFGWSSSVAGLVQSSFFWGYALSQLPGGWLSKIFGGRKVLEIGVFTWSFATALVPLLAGFMPGLIFSRILVGIGEGVSPSAATDLIARTIPVKERSRAVGFVFGGLSLGSVMGLLLAPPIIETFNWESVFYLFGLLGVGWFVGFQFLNEEEVSYKGNEISTSHKSENATKEELGSSLKEIPWKSFFQSPAVWAMIYTHFCGSWGHYTCLSWLPTYFSEALSLNLTEAAWVSILPPLASIVVTSLASQFADYLITNGVDTTTVRKICQTIAFVAPAICMTLSSVDIGLPPWEIVGILTAGLALSSFALSGLYCTHQDISPEYASILLGITNTVGAVPGIVGVALTGFLLDSTHSWTMSLFVPSIFFYLTGTVVWLAFASSKL",
    "response#3": "TSLIPFLEHNDANRALMGSNMQRQTVPVLGAHNFIVRTGLDTKFFSDISSIPISQINGFNFESNNNYCYFYKIDNLITLNNISYNYIKKTNHNSLYINESIKKKNPWHQQGDFLINNSVIENGKLALGPNLLLAYLPWHGYNYEDAVIINERCVSQKILTSLHIIG"
  },
  {
    "reference": "METVLSAPEVFIALVVAAHAAVLALRLSISLYEA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The protein should be designed to localize specifically to photosystem I, plasma membrane-derived thylakoid membrane in order to perform its role in photosynthesis more effectively. The designed protein sequence is ",
    "response#1": "VFPAGRDPQWGNLETPVNASPLVKWFINNLPAYRPGLTPFRRGLEVGMAHGYFLFGPFAKLGPLRDAANANLAGLLGAIGLVVLFTLSLSLYANSNPPKALASVTVPNPPDAFQSKEGWNNFASAFLIGGIGGAVVAYFLTSNFL",
    "response#2": "VFPAGRDPQWGNLETPVNASPLVKWFINNLPAYRPGLTPFRRGLEVGMAHGYFLFGPFAKLGPLRDAANANLAGLLGAIGLVVLFTLSLSLYANSNPPKALASVTVPNPPDAFQSKEGWNNFASAFLIGGIGGAVVAYFLTSNFL",
    "response#3": "VFPAGRDPQWGNLETPVNASPLVKWFINNLPAYRPGLTPFRRGLEVGMAHGYFLFGPFAKLGPLRDAANANLAGLLGAIGLVVLFTLSLSLYANSNPPKALASVTVPNPPDAFQSKEGWNNFASAFLIGGIGGAVVAYFLTSNFLR"
  },
  {
    "reference": "MARHKIALIGSGMIGGTLAHLVGLKELGDVVLFDIAEGIPQGKGLDIAESAPVEGFDAKFLGTNDYAAIEGADVCIVTAGVPRKPGMSRDDLLGINLKVMEQVGAGIKKYAPNAFVICITNPLDAMVWALQKFSGLPKSHVVGMAGVLDSARFRYFLAEEFKVSVEDVTGFVLGGHGDSMVPLIRYSTVAGIPIPDLVKMGWTTQEKIDQIVQRTRDGGAEIVGLLKSGSAFYAPASSAIAMAEAYLKDKKRVLPCAAHVSGQYGVKDLYVGVPTVIGAGGVERIIEIDLNKTEQKMFENSVASVEGLCKACGDIAPSLKN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should be able to enhance the efficiency of carboxylic acid metabolic process.2. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in (S)-malate + NAD(+) = H(+) + NADH + oxaloacetate are properly positioned for optimal catalysis.3. The designed protein should have a high specificity for substrate, NAD(+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "LDSARFRHFLAEEFGVSVEDVTAFVLGGHGDDMVPLTRYSTVAGVPLTDLVKLGWTTQEKLDAMVERTRKGGGEIVNLLKTGSAFYAPAASAIAMAESYLRDKKRVLPCAAYLDGQYGIDGLYVGVPVVIGENGVERVLEVTFNDDEKAMFEKSVNSVKGLIEA",
    "response#2": "IQGTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAGAGSAGIGVLNAARKTMARMLGNTEIAFESARSQFWVVDAKGLITEARENVDPDARPFARKIKEIERQGLSEGATLAEVVREVKPDVLLGLSACGGLFSKEVLEALKHSTSTRPAIFPMSNPTRNAECTPEEAFSILGENIIFASGSPFKDVDLGNGHVGHCNQANNMFLFPGIGLGTLLSGSRIVSDGMLQAAAECLAAYITEEEVLKGIIYPSISRIRDITKEVAAAVVKEAR",
    "response#3": "IQGTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAGAGSAGIGVLNAARKTMARMLGNTEIAFESARSQFWVVDAKGLITEARENVDPDARPFARKIKEIERQGLSEGATLAEVVREVKPDVLLGLSACGGLFSKEVLEALKHSTSTRPAIFPMSNPTRNAECTPEEAFSILGENIIFASGSPFKDVDLGNGHVGHCNQANNMFLFPGIGLGTLLSGSRIVSDGMLQAAAECLAAYITEEEVLKGIIYPSISRIRDITKEVAAAVVKEAE"
  },
  {
    "reference": "MSGNDEEFNDEFLLAIDSIETTLKKADMYRPLPPPYLPTFLPAPPPSTKISSSLSHPMQLQSSAGQQRKQIQVPDPFLSYSPPRELSQRVVSGFNDALMDYSNSTVVTAAKPISPTTSNRRCDSEKDLEIDRLKKELERVSKQLLDVEQECSQLKKGKSKETESRNLCADDNRGQCSTVHASKRIDLEPDVATSSVNHRENDSRMALDDKRSFKTTGVQADVANHSDLSKKLLDIWRTSNYQDPRKNLISELLLACSTDLQILFSFMKISTPPQELNKQEAKTSSDRQSSKALESEKVYQLYSAVTKISYGFVNLKTLVEPLLDLCKAETAVLVHRSLRVLHVLLEHICGDEKRFEASWDANWHSLFKLMNQIASKRTEQDVKQEALSIMNIIVMSTDAYTARESFVSKEVFESISLLLRKEGGLHVRKEAIHLFYLLLNCPKLYDTFDSLHEEKNSSDTENDSEGNFFALEAFGKIFEGLADCLTSPRKTSEDLELCRNVIMILALAASSGNSGYELLSSHKLPQDSSFLMLILHLLVAEIDSESTEFHPKAEIFKARTLLMREILILLNRLVSGLSSSATILKELTTSRDMASLTVDAATRLSRKRNLLGKPESSVERMRNTEIMDLARIFKKRVFAFLGDNTI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein's functional design necessitates the inclusion of a Nuclear localization signal motif.2. For general function, the protein need meet that Involved in cell- cycle G2/M arrest in response to DNA damage; Required for aluminum-dependent gene regulation and root growth inhibition in an ATR-dependent manner by halting cell cycle progression and triggering loss of the quiescent center (QC).; Required for tolerance to DNA-damaging and cross-linking agents such as UVB irradiation, gamma-radiation, aphidicolin, ionizing radiation and hydroxyurea (HU), cisplatin (CDDP) and mitomycin C (MMC)3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.4. A protein that contributes to response to aluminum ion, positive regulation of cell cycle G2/M phase transition, response to gamma radiation, response to cisplatin, response to hydroxyurea by functioning in nucleus, cytoplasm with protein dimerization activity, phosphoprotein phosphatase activity.5. The presence of Phosphatase tensin-type domains is critical for the interaction of the designed protein with its intended targets.6. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "MSLALNDLLICCRHLEHDRATERKKEVENFKRLIRDPEIIQYLDRYSDSKQGKYLNWDAVFRFLQKYIQKETECLRIPRPNISASTQAARQKKMQEISSLVKYFIKCANKRAPRLKCQELLNYIMDTVKDSSNGAVYGADYSNILLKDILSVRKYWCEISQQQWQNYAGEHGFNLEFNDSGTAKSVTSTYSVKLGKLFCQLAKTTPIGVLVKEEPPQGAVIRATAVYKKTEHVGEVVKRCPHHQSEDLSDNKSHLIRVEGSQLAQYFEDPNTRRHSVTVPYERPQLGSEMTTILLSFMCNSSCMGGMNRRPILTILTLETTEGEVLGRRCFEVRVCACPGRDRKTEEGGLEEADLTLPLSQDTFHDLWNNVFLSSPPECQIVHRDVKPSNFLQ",
    "response#2": "MSLALNDLLICCRHLEHDRATERKKEVENFKRLIRDPEIIQYLDRYSDSKQGKYLNWDAVFRFLQKYIQKETECLRIPRPNISASTQAARQKKMQEISSLVKYFIKCANKRAPRLKCQELLNYIMDTVKDSSNGAVYGADYSNILLKDILSVRKYWCEISQQQWQNYAGEHGFNLEFNDSGTAKSVTSTYSVKLGKLFCQLAKTTPIGVLVKEEPPQGAVIRATAVYKKTEHVGEVVKRCPHHQSEDLSDNKSHLIRVEGSQLAQYFEDPNTRRHSVTVPYERPQLGSEMTTILLSFMCNSSCMGGMNRRPILTILTLETTEGEVLGRRCFEVRVCACPGRDRKTEEGEAGDFKIKDKIGEGTFSSVYLATAQLQEGHEEKIALKHLIPTSHPMRIAAELQCLTVAGGQDNVMGLKYCFRKNDHVVIAMPYLEHESFLDILNSLSFQEVREYMYNLFVALKRIHQFGIVHRDVKPSNFLYNRRLKKYALVDFGLAQGTRDTKIELLKFVQSEAQQEDCSRNKYHGVVGHKGLLSRPAPKTVDQQCTPKTSVKRSYTQVHIKQGKDGKERSVGLSVQRSVFGERNFNIHSSISHESPAEKLIKQSKTVDIISRKLATKKTAISTKAMNSVMRETARSCPAVLTCDCYGSDRVCSVCLSRRQQVAPRAGTPGFRAPEVLTKCPDQTTAIDMWSAGVIFLSLLSGRYPFYKASDDLTALAQIMTIRGSRETIQAAKAFGKSVLCSKEVPAQDLRALCERLRGLDSTTPRSASGPPGNASYDPAASKNTDHKASRVQAAQAQHSEDSLYKRDNDGYWSHPKDCTSNSEGWDSVPDEAYDLLDKLLDLNPASRITAEAALLHAF",
    "response#3": "FKIKDKIGEGTFSSVYLATAQLQEGHEEKIALKHLIPTSHPMRIAAELQCLTVAGGQDNVMGLKYCFRKNDHVVIAMPYLEHESFLDILNSLSFQEVREYMYNLFVALKRIHQFGIVHRDVKPSNFLYNRRLKKYALVDFGLAQGTRDTKIELLKFVQSEAQQEDCSRNKYHGVVGHKGLLSRPAPKTVDQQCTPKTSVKRSYTQVHIKQGKDGKERSVGLSVQRSVFGERNFNIHSSISHESPAEKLIKQSKTVDIISRKLATKKTAISTKAMNSVMRETARSCPAVLTCDCYGSDRVCSVCLSRRQQVAPRAGTPGFRAPEVLTKCPDQTTAIDMWSAGVIFLSLLSGRYPFYKASDDLTALAQIMTIRGSRETIQAAKAFGKSVLCSKEVPAQDLRALCERLRGLDSTTPRSASGPPGNASYDPAASKNTDHKASRVQAAQAQHSEDSLYKRDNDGYWSHPKDCTSNSEGWDSVPDEAYDLLDKLLDLNPASRITAEAALLHAFSN"
  },
  {
    "reference": "MRPAQTNQFDYVKIGLASPERIRQWGERTLPNGQVVGEVTKPETINYRTLKPEMDGLFCERIFGPAKDWECHCGKYKRVRHRGIVCERCGVEVTESRVRRHRMGYIKLAAPVAHVWYLKGIPSYISILLDMPLRDVEQIVYFNSYVVLSPGNAETLTYKQLLSEDQWLEIEDQIYSEDSQLQGVEVGIGAEALLRLLADINLEQEAESLREEIGSAKGQKRAKLIKRLRVIDNFIATGSKPEWMVMTVIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLARLQEILAPEIIVRNEKRMLQEAVDALIDNGRRGRTVVGANNRPLKSLSDIIEGKQGRFRQNLLGKRVDYSGRSVIVVGPKLKIHQCGLPREMAIELFQPFVINRLIRSGMVNNIKAAKKLISRNDPSVWDVLEEVIEGHPVMLNRAPTLHRLGIQAFEPILVEGRAIQLHPLVCPAFNADFDGDQMAVHVPLSLESQAEARLLMLASNNILSPATGRPIITPSQDMVLGAYYLTAENPGATKGAGKYFASLDDVIMAFQQEQIDLHAYIYVRFDGEVESDQPDTDPLEVTNNDDGSRTVLYKYRRVREDAQGNLISQYVRTTPGRVIYNKAIQEALAS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a low Km value for a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +   RNA(n+1).2. The protein must exhibit the following characteristics: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates..3. The presence of Zn(2+) is necessary for the protein to function in the desired environment.4. The ideal protein would be one that plays a critical role in DNA-templated transcription through zinc ion binding, DNA-directed 5'-3' RNA polymerase activity, DNA binding, magnesium ion binding within DNA-directed RNA polymerase complex.5. The Zn(2+), Mg(2+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "EWMVLCLLPVLPPELRPIIQVDGGKLMSSDINELYRRVIYRNNTLIDLLTTSRSTPGELVICQEKLVQEAVDTLLDNGIRGQPMRDGHNKVYKSFSDIIEGKEGRFRETLLGKRVDYSGRSVIIVGPSLSLHRCGLPGEIAIELFQTFLIRGLIRKHFASNIGIAKSKIRQKEPIVWEILQEVMQGHPVLLNRAPTLHRLGIQAFQPILVEGRAICLHPLVCKGFNADFDGDQMAVHVPLSLEAQAEARLLMFSHTNLLSPAIADPISVPTQDMLIGLYILLAAHNLLDPANGKTIVFPTQDMVLGLYYLTKERALPEGKKERLYSSVPEVLMAAECHAVGWQEPVLIDYETEPGKIETVRTTPGRILFNEEMPEGVPFTNYALNDKKIRKLIENVFKDKGPWLAVQLLDKLKAVGYKYATYFGATLSMEDMLVPSSGNNILSPANGSPVIVPSQDIVLGLYYMTVERSFEKGEGMVFCAPWEVISAIDNNQVNIHAKIKVRMEDGNIYNTTAGRILVWEGLPKGLKFEYVNCVLTKKNIATLVGNAYRDAGIKATVILCDRLKDIGYEYATRAGITIGVKDMVVPTTKTEEWMILDVIPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKKLMELGAPDIIIRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKIYQCGLPKEMALELFKPFVMKKLVEKGICNNIKNAKKAVERQRSEVWDILEEVIKDHPVLLNRAPTLHRLGIQAFEPVLVEGRAIRLHPLVCTAYNADFDGDQMAVHVPLSAEAQAEARFLMLSANNLLKPADGKPIVVPTQDMVLGIYYLMFSHTNLLSPATGDPISVP",
    "response#2": "EWMILEVLPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLAEMIKGKGGRFRQNLLGKRVDYSGRSVIVVGPTLKLHQCGLPKLMALELFKPFIFHKLETMGIATTIKAAKKEVESQTPVVWDILEEVIREHPVMLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLFPANGDPSIVPSQDVVLGLYYTLAGINNIKNAKKAVERQRSEVWDILEEVIKDHPVLLNRAPTLHRLGIQAFEPVLVEGRAIRLHPLVCTAYNADFDGDQMAVHVPLSAEAQAEARFLMLASWNVLFPANGEPSIVPSQDVVLGLYYATRDKINGKGEGMVFANITEVVRAYEAGQVELASRVAVRITEYEIVDKKAEGDARFAGKTKIYQTSVGRAILSEILPKGMSFEEINKPLKKKEISRLINTSFRKCGLRETVIFADRLLQSGFRLATNAGISVAIDDMVIPTEKDELIAEADKEVDEIIEQFQSGLVISDEERYEQVISIWNETKDKLTEELIQNLDEFNPIFMMASSGARGSKNQISQLAGMRGLMANPSGKTIEMPIKSNFREEQFDAIKIGLASPEKIRSWSYGEVKKPETINYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTLAKVRRERMGHIELAAPTAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVIEPGMTPLKKSQIMSEDDYLAKCDEYGEGEFVAMMGAEGIRELLRGIDIEKQIEQIRAELQATGSEAKIKKFAKRLKVLEAFQRSGIKPEWMILEVLPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLAEMIKGKGGRFRQN",
    "response#3": "EWMIMTVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLMELNAPEIIVRNEKRMLQEAVDALIDNGRRGRPVSGKGKHRLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPTLQLHQCGLPKKMALELFKPFVMRRLVDKGFAHNIKSAKRFVERVRPEVWDVLEEVIKDYLVLLNRAPSLHRLSIQAFEAKLIEGSAIQLHPLVCAAFNADFDGDQMAVHVPLSRKAQEEARRRMISTYNLLSPATGDPIITPSQDIVLGCFYLLAAHNILSPAHGKPIIVPSQDMVLGIYYMTRERHFAKGDGKIFASPEEVRIAYDAGEVDLQARISVRMKNILSAASEQPAIIETTVGRILLREILPEMVPFSSINKVMSKKELVNLIDVCYRLAGNKETVILADRLKETGFRYSTLAGISICMNDIIVPPPKSSLLGKRVDYSGRSVIVVGPKLKLHQCGLPKKMALELFKPFIYSELEKI"
  },
  {
    "reference": "MGYRIAVVGATGNVGREMLDILDERGFPADEVVALASRRSQGTEVSFGDKTLKVQALEHFDFANADIALMSAGGSVSLEWAPKIAAKGCVVIDNSSAWRTDPDVPLIVPEVNADAVAGFAKKNIIANPNCSTIQLVVALKPLHERAKIKRVVVATYQSVSGAGKDAMDELFNQTRAVFVADPIETKKFPKRIAFNCIPHIDVFMEDGTTKEEWKMVAETKKILDPKIKLSATCVRVPVFVSHSEAVNIEFEEELSAEEARNILREAPGCLVIDKHEPGGYMTPYECAGEDATYISRIREDPTVDSGLSMWVVADNLRKGAALNTVQIAELLVNRKLLENRKAA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should contain one or more Semialdehyde dehydrogenase NAD-binding domains that are essential for its function.2. A key design requirement for the protein is the optimization of the Acyl-thioester intermediate to achieve high catalytic efficiency for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.3. The designed protein must possess aspartate-semialdehyde dehydrogenase activity, NAD binding to support 'de novo' L-methionine biosynthetic process, lysine biosynthetic process via diaminopimelate, isoleucine biosynthetic process, threonine biosynthetic process under certain conditions.4. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..5. The protein should have a high affinity for phosphate, NADP(+), substrate.6. Ideal protein should have a binding affinity for key enzymes in the L-threonine from L-aspartate: step 2/5, L-threonine biosynthesis, Amino-acid biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MTNLAVVGATGAVGRIVLEQLALRKLPINRLKLLASQRSVGKEVRFADETLKVQLLEPDAFQGIDIVVASTPDEVSAEFASWATDAGAVVVDESGYWRMDPKVPLVIPEVNPEAIHDLAPIHRAVGIERINVATYQSVSGAGRSGGEELGRQTAALLNFQDPEPSKFPVQIAFNLIPHIDDFQDNGYTKEEMKMVWETRKILEDASIQVNPTAVRVPVFFGHAEAVHIETRERISADAARRLLQAAPGVVVVDERKPGGYPTPAGHAAGKDPVFVGRIREDISHPRGLDLWVVADNIRHATNIVAGTCVRVPVFTGHLAPLHRKYKIKRIVAATYQSVTGTGVKAVTQMENERKGIKGEMAYAHPIDMNCFPHGGSFQPDTYTTEEQKLIDETRKILEDQSIMVTATVVRIPVVGGHSEAVNVEFEKEYDLQEAISLMSKFPGVVIYDNPAENKYPMPILSHNRDEVFVGRIRRDHSREKCLNLWIVSDNIRGASGNALAPIHRAVGIERINVATYQSVSGAGRSGGEELGRQTAALLNFQDPEPSKFPVQIAFNLIPHIDDFQDNGYTKEEMKMVWETRKILEDASIQVNPTAVRVPVFFGHAEAVHIETRERISADAARRLLQAAPGVVVVDERKPGGYPTPAGHAAGKDPVFVGRIREDISHPRGLDLWVVADNIRTPISGTVELATTTGLAPIHRAVGIERINVATYQSVSGAGRSGGEELGRQTAALLNFQDPEPSKFPVQIAFNLIPHIDDFQDNGYTKEEMKMVWETRKILEDASIQVNPTAVRVPVFFGHAEAVHIETRERISADAARRLLQAAPGVVVVDERKPGGYPTPAGHAAGKDPVFVGRIREDISHPRGLDLWVVADNIR",
    "response#2": "MAAVVGATGAVGEALLEILAERRFPVGELVALASERSAGGQLTYAGRSVPVRNLAAFDFAGVDIAFFSAGGSVSRVHAPRAAAAGAVVIDNTSEFRYQDDIPLVVSEVNPHAIADHKPPKGIIANPNCTTIALVGATGAVGRIVLDQIVSRKLPYKRLKLLASERSVGKEVRVGDQTIKVELLEPDAFKNVDLVIGSTPDEVAAEFAPWAVAAGAVVVDESGFWRMDAKVPLVIPEVNPDAVDHVRRGVAGNALQIAEVLVAGL",
    "response#3": "MSITIALVGATGAVGRIVLDQIVSRKLPYKRLKLLASERSVGKEVRVGDQTIKVELLEPDAFKNVDLVIGSTPDEVAAEFAPWAVAAGAVVVDESGFWRMDAKVPLVIPEVNPDAVDHAVRWHRGIIANPNCSTIQMVVVLAPLHREAGLERLIISTYQAVSGAGLAGVDELDKQVRQVVDRAAELTHDGSAITFPEPVKFVRPVAFNVLPLAGSIVDDGSFETDEEQKLRNESRKILGIPNLRVAGTCVRVPVFTGHSLSISAEFARPLSVTRALDLLSVAPGVEVTDIPTPLLAAGADPSYVGRVRQDPTVDGGRGLTLFVSSDNLRKGAALNAVQIAQRMVEIGWLGS"
  },
  {
    "reference": "MAMIKNSIREYKLVVVGGGGVGKSALTIQLIQSHFVDEYDPTIEDSYRKQVVIDGQVSILDILDTAGQEEYSAMREQYMRTGEGFLLVYSVTSRTSFEELMTYYQQILRVKDVEYVPIFVVGNKSDLEGERQVSFEEGAELARHFNASFLETSAKQAINVEESFYGLARLVRDDGIHAGKRLESSPEDPANAAGAEPSAGYSKDDNALDPEAVQGTLANSSVGSRAPGAVDHKGETSAVSGSLRSSRAQQQSLNTDGVAAKKSTGCCVIL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should emphasize Polar residues compositional bias.2. A protein that contributes to regulation of cytoplasmic mRNA processing body assembly, positive regulation of transcription by galactose, positive regulation of protein import into nucleus, signal transduction involved in positive regulation of conjugation with cellular fusion, positive regulation of pseudohyphal growth by functioning in nucleus, division septum, endoplasmic reticulum membrane, actin fusion focus, plasma membrane with UTP binding, GTPase activity, GTP binding, GDP binding, CTP binding. The designed protein sequence is ",
    "response#1": "LKARKVHFAEKVTVHFLAVWAGPAQAARRGPWEQFARDRSRFARRIAQAEEKLGPYLTPDSRARAWARL",
    "response#2": "MSDGKHRNLCRYYEDFFNFQTKFLELFPNEAGRGDERRVIPYMPGPVDDVNELISSQRAMDLDVYLKEMCRLPARLLENELVKLFFG",
    "response#3": "LKARKVHFAEKVTVHFLAVWAGPAQAARRGPWEQFARDRSRFARRIAQAEEKLGPYLTPDSRARAWARL"
  },
  {
    "reference": "MRVGVLTGGGDCPGLNAAIRAIVRKGIDHYGHAIVGFRDGWRGPLENDHEELTIESTRGILPRGGTILGTSRTNPFKREDGPERIRETMAGLHLDGLIAIGGEDTLGAGASLHGDHGVAVLGVPKTIDNDLAATDVTFGFDTALQVATEAIDRLHTTAESHHRVMVVEVMGRHAGWIALHSGLAGGGDVILIPERPFDIEDVCRLIRRRHSRGRNFSIVVVAEGALPEEGTMEVAEGERDEFGHVRLGGIGHRLEREIEVRTGFETRAVVLGHIQRGGTPTAFDRVLATRLGVAAIDAAHEGRWGTMPALRGTRITLVPLSEAVEQLRTVPPEDYEVAAPFFG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a high affinity for diphosphate, Mg(2+), substrate.2. A key design requirement for the protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for beta-D-fructose 6-phosphate + diphosphate = beta-D-fructose   1,6-bisphosphate + H(+) + phosphate.3. The protein must exhibit the following characteristics: Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis; Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis.4. The ideal protein would be one that plays a critical role in fructose 6-phosphate metabolic process through diphosphate-fructose-6-phosphate 1-phosphotransferase activity, 6-phosphofructokinase activity, ATP binding, metal ion binding within cytoplasm.5. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.6. A protein that can modulate the overall flux of metabolites through the D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 3/4, Carbohydrate degradation, glycolysis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "MTKVGLLTGGGDCPGLNAAIRAVVRKATSVNWEVLGIKNGWKGLIEGNVEPLTKYSVSGILPKGGTILGTSRTNPFKDYENTQKLLDNFKRYELNGIIAIGGEDTLGVAMRLYKEYSIPVIGIPKTIDNDIAGTDLTFGFDTAVSIVTESIDRLHSTAESHHRIMVVEVMGRHAGWIATISGIAGGADIILVPETPFDINNVCNILTKRHEKGKHFSIVVVAEGATPKGSEIVITQSNKKDEFGHVRLGGIGSVLAQEIEKRTGIETRVTILGYIQRGGTPTAFDRVLATRYGVLAMEAVEKGFFGTMVALRGTDIVRVPLAEGTKELKTVPPDRYAEAEVFFG",
    "response#2": "MDKIGILTGGGDAPGLNAVIRGATVKGIKEGYEVIGFRLGWAGTLNKDYQPLTLSDVEDIHRQGGTILGSSRTNPAKLEDGLQKVKNNLEELDIYALIASGGEDTLGVANKLYGLGVRVVGVPKTIDNDLSATDYTFGFDTAINRVMEALDRLHTTAKSHQRVMVVEIMGRHSGWMALYGGIAGGAHMILLPEEEFDYQDVCDLIKRRYDEGNKYAIIAVAEGSVCPQLEKEGQEAPRDDFGHVVFTKRKRGVAEILADKIEEVTGIETRQVVLGHLQRGGDPSAFDRVLATRLGVKAVEMVKEGKFGLVMGLMGNRIVAIPLAEALKTLKTVDPELYEIAEVFFG",
    "response#3": "MKVGLLTGGGDCPGLNAAIRAVVRKATSVNWEVLGIKNGWKGLIEGNVEPLTKYSVSGILPKGGTILGTSRTNPFKDYENTQKLLDNFKRYELNGIIAIGGEDTLGVAMRLYKEYSIPVIGIPKTIDNDIAGTDLTFGFDTAVSIVTESIDRLHSTAESHHRIMVVEVMGRHAGWIATISGIAGGADIILVPETPFDINNVCNILTKRHEKGKHFSIVVVAEGATPKGSEIVITQSNKKDEFGHVRLGGIGSVLAQEIEKRTGIETRVTILGYIQRGGTPTAFDRVLATRYGVLAMEAVEEGAYGQMVALRGTEIVRVPLAEGTRELKTVPPDRYAEAEVFFG"
  },
  {
    "reference": "MDHSNHSHGTSSDGEAKCPFTGHSSEQGSKGGTQNQDWWPNLLNLNILRQHGVASNPMGSDFNYAKAFASLDLDALKKDLHAVMTDSKEWWPADYGHYGPFFIRMAWHSAGTYRITDGRGGGGTGTQRFAPLNSWPDNTNLDKARMLLWPIKQKYGKKLSWADLMILTGNCALESMGFKTFGFAGGREDVWQPEEDIYWGSEGEWLASKRYSGDRELDNPLAAVQMGLIYVNPQGPDGNPDPLASARDIRETFGRMAMDDEETVALIAGGHTFGKTHGAADADEHVGTEPAGASIEQQGMGWNNSFGTGRGAHAITSGLEGAWTSTPVQWSNSYFDNLFKFDWELTKSPAGAFQWKPKNGGGAGTVPDAHDPSLSHAPTMLTSDLALREDPAYGAISKRFYENPDQFSDAFARAWFKLTHRDMGPVALYVGKDVPSEIMIWQDPIPAATAPIVNATQIADLKGRIGASGLSVAQLVRTAWASASTFRGSDKRGGANGGRLRLAPQKYWAVNNPSELGHVLGAYESIQTAFNSENAQGAQVSMADLMVLGGCVGVEQAAHNAGHSISVPFTPGRTDATQEQTDVASFAVLEPFADGFRNYLQGASSIAAEEWLIDKAQLMTLTAPELTVLVGGMRSLNANFDGSETGILSHRKESLTTDFFTNLLDFSTVWKAQPGSNNLYNGSGRTSGDAKWTASRVDLVFGSNSELRALAEVYACGDSEEQFMHDFVAAWNKVMNLDRFDLRA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have catalase activity, metal ion binding to facilitate hydrogen peroxide catabolic process, response to oxidative stress.2. The Proton acceptor active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The designed protein should have a binding site that is specific to the target heme b ligand.4. The protein requires heme b to activate a critical catalytic site.5. The protein must exhibit the following characteristics: Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity..6. The presence of Plant heme peroxidase family profile domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "AKAWFKLTHRDMGPLSKYIWEKEPKETLIWQDPIPSVDHKLIGEKEIKSLKGKILGSGLTIPELVRTAWAAAASFRSTDMRGGANGGRLRLSPQREWTVNNPDELKKVLKELTEIQREFNESGKKVSLADVIVLGGVAAVEEAAKKAGVKVSVPFTPGRMDASQESTDVKSFAVLEPKADAFRNYYGDGNYLSPTEALVDRANMLSLTVPEMTVLVGGLRALNANSANSKHGVLTQRPGVLSNDFFVNLLDMSTKWQKSSDNAAVYEGIDRKTGAKKWSATSVDLIFGSHSELRSVAEVYASK",
    "response#2": "AKAWFKLTHRDMGPLSKYIWEKEPKETLIWQDPIPSVDHKLIGEKEIKSLKGKILGSGLTIPELVRTAWAAAASFRSTDMRGGANGGRLRLSPQREWTVNNPDELKKVLKELTEIQREFNESGKKVSLADVIVLGGVAAVEEAAKKAGVKVSVPFTPGRMDASQESTDVKSFAVLEPKADAFRNYYGDGNYLSPTEALVDRANMLSLTVPEMTVLVGGLRALNANSANSKHGVLTQRPGVLSNDFFVNLLDMSTKWQKSSDNAAVYEGIDRKTGAKKWSATSVDLIFGSHSELRSVAEVYASLD",
    "response#3": "AKAWFKLTHRDMGPLSKYIWEKEPKETLIWQDPIPSVDHKLIGEKEIKSLKGKILGSGLTIPELVRTAWAAAASFRSTDMRGGANGGRLRLSPQREWTVNNPDELKKVLKELTEIQREFNESGKKVSLADVIVLGGVAAVEEAAKKAGVKVSVPFTPGRMDASQESTDVKSFAVLEPKADAFRNYYGDGNYLSPTEALVDRANMLSLTVPEMTVLVGGLRALNANSANSKHGVLTQRPGVLSNDFFVNLLDMSTKWQKSSDNAAVYEGIDRKTGAKKWSATSVDLIFGSHSELRSVAEVYASS"
  },
  {
    "reference": "MVAVAILAAGRGTRMKSDLPKVLHSVGGKPMVERVLDGLAAVQPERIFVIVGFGQEKVQTALTHIPNLTFVHQAEQLGTGHAVQQVIPHLEGFEGDLLVLNGDVPLLRPETIYTLVQTHRDNQNAATLLTAQFADPTGYGRVFCTEQSIVTEIVEHRDCSPAQRQNPRINAGVYCFNWPKLMEVLPHLQSDNDQQEYYLTDVVKDLSPAMAVDVADCQEIFGINSRQHLAEAYAILQDRIKTQWMAAGVTLIDPDSITIDTTVHIEPDVVIEPQTHLRGHTSIGTGSRIGPGSMIEDSQIGPNTTITFSVISDSIVAAGAKVGPYAHLRGHAVVGENCRIGNFVELKKATLGQGTNVAHLSYLGDATLGEKVNVGAGTITANYDGYKKHPTVIGDRTKTGSNSVLVAPITVGSDVTIAAGSVVRKDVPDDCLVVSRAPQKIHEGWKPAHLRDTSSEAKLPNP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that contributes to UDP-N-acetylglucosamine biosynthetic process by functioning in carboxysome with glucosamine-1-phosphate N-acetyltransferase activity, magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity.2. The protein contains novel MobA-like NTP transferase domains that confer a unique function or activity.3. A protein that can modulate the overall flux of metabolites through the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, Nucleotide-sugar biosynthesis pathway, enhancing or suppressing its activity as needed.4. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation. The designed protein sequence is ",
    "response#1": "VIVLAAGEGTRMKSRTSKVLHELCGRSMLGHVLAAARELDPGRLIVVVGHRREQVAAHLAEHVPAAEPVVQERQGGTGHAVRTVLDQVGALTGTVVVTNGDHPLLRGETLAELVRTHETEGNAVTVLTTFVADPSGYGRIVRDANGNVTAIVEHKDASDEQRAVDEVNSGIYAFDASVLRDGLAKLSTDNAQGEKYLTDVIGIARDAGGRVTAHRLEDAWQTEGVNDRVQLAAVGRELNRRTVEHWMREGVTIIDPIATYIDATVQVGQDTIIYPGTFLEGHTIIAAGCTLGPGTTLRDCQVGEGSQIIHTVALESQIGAGCQVGPFTYLRPGTILEDRTKVGDFVELKATRIGTGSKVPHLTYLGDTTVGTGVNIGAGTITCNYDGQKKWPTVIEDGAFIGSNSNLVAPVRVGAGALVGAGSTITEDVPAGSLALARGRQRNIEGWVERKRPGSAAAQAAEAASAQESDE",
    "response#2": "MTSPAILLAAGLGTRMRSKQPKATHALAGRPMLNHLIAACEGVFDRIVVVVGPDMPTLERAAAPHPTVVQAERLGTGHAALQAAPLLEGFEGDVAVLYADNPLITQDTLKRLRAVREEAGLALLALRPAGRDLGLLTVRMDDPSGYGRIVRENGAVTGIVEHKDATAEQLAIQEINTGILVANGADLRRWLGRLSNDNAQGEYYLTDVIAMAHQDGVPIAAAQPADPVEVEGVNDRLQLARLERAYQARQAERLMLAGVTIQDPANTYISADAEIGRDTVLMPGTVIEGRTVIGEDCLIGPGSQIRDSVIGDRTAIRQSVVMSSKIGEDTAVGPFAHIRPETDLGNSVKIGNFVEVKKSNLGEGSKVSHLSYIGDSDIGERVNIGCGTITVNYDGVNKFRTTVEDDAFVGCNSNLVAPVTVGKGAFVAAGSTVTKNVPENALAIARSRQRNIAGWHRPKKK",
    "response#3": "VVLAAGLGTRMKSSLPKVLHKLVGVPMIQHVVKTLQETGINDIIVVLGYRGELVEEELGGSCRVVYQQQQLGTGHALLQTMSELQNYKDADCLVVCGDTPLLRSETLLGLISAHQQQKAQATVLTVILANPAGYGRIIRSADGGVTAIVEQKDATPEQLAIREINTGILVAPVRHLRQWLARLDNNNAQGEYYLTDIIAMAVADGVPVVSAHPADEWEVAGVNSKVQLAELERIHQLNQARRLMEQGVTIQDPANTYISADAEIGRDTVLMPGTVIEGRTVIGEDCLIGPGSQIRDSVIGDRTAIRQSVVMSSKIGEDTAVGPFAHIRPETDLGNSVKIGNFVEVKKSNLGEGSKVSHLSYIGDSDIGERVNIGCGTITVNYDGVNKFRTTVEDDAFVGCNSNLVAPVTVGKGAFVAAGSTVTKNVPENALAIARSRQTNISGWVRPVKR"
  },
  {
    "reference": "MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVLIAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQRGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHGEYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFPTDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYFPANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNWPRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESEGRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLLGERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEYLMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAAELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDRLVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEMISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKERIKAMRANVDILTLTATPIPRTLNMAMS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have a high specificity for ATP binding, with minimal off-target effects.2. strand-strand-helix-strand-strand-helix-strand-helix-helix-strand-helix-helix-strand-helix-strand is the desired arrangement for the engineered protein.3. A protein that contributes to transcription-coupled nucleotide-excision repair, DNA damage recognition, nucleotide-excision repair, preincision complex assembly by functioning in DNA repair complex, cytosol with RNA polymerase core enzyme binding, DNA translocase activity.4. The protein must exhibit the following characteristics: Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions; Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site; Can also dissociate RNAP that is blocked by low concentration of nucleoside triphosphates or by physical obstruction, such as bound proteins. The designed protein sequence is ",
    "response#1": "MDQKLSKLVEELTTSGEPRLNPEKMKELKKICKSSEEQLSRAYRLLIAQLTQEHAEIRLSAFQIVEELFVRSHQFRMLVVSNFQEFLELTLGTDPAQPLPPPREAAQRLRQATTRAVEGWNEKFGEAYKKLALGYHFLRHNAQRAWVRRVLKGKSFAAIAPTGVGKTTFGVLMAVYLASRGEKSYIVVPTTPLVKMVEERALRLADAAGVPARIVALHSRLSQKQRRRLLERVAEGDFDILITTSQYLVRNAETLAGLREQWGRGFRFVFVDDVDAVLKSGRSSTEDQLTAINDIREDLTNGMLMDRLICGDVGFGKTEVAMRAVFMVAKSLNEYLPQVAVVVPTTILCSQHFSRFIERFKGFGLNIKQLSSVISSKEANIIRLELASGKINIIIGTHALLHKNTKFFNLKLLIIDEEQHFGVSQKEFLKSLKSSTHVLAMSATPIPRTLLVERLAELLRSRGVAAEAFTSQNPGALERFRSGETQVLVGVAVYYGVAVRGLDLPERIRYAVFAGVPRLRFSARFEDPHPLSILRALAVLAEHAPRDVAERAQTLLGKLRALARRLSQAALARVAEELRSGRPGSDAAREFASALEWLRDTMRREDVWRALERADDIAVVREEGRAYILVPDVATYIQASGRTSRLRSGEVVMFTATRRVLPKNPDKHLEAGCDLLKQAFARAAILSNEKFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCENVRMMLTDSVSSVQIEDAASQSAAYVVMPM",
    "response#2": "MLSTGTKSLDSLLGGGFAPGVLTQVYGPYASGKTTLALQTGLLSGKKVAYVDTEGGFSPERLVQMAETRGLNPEEALSRFILFTPSDFKEQRRVIGSLKKTVDSNFALVVVDSITAHYRAEENRSGLIAELSRQLQVLLWIARKHNIPVIVINQFPFSETEDQLTAINDIREDLTNGMLMDRLICGDVGFGKTEVAMRAVFMVAKSLNEYLPQVAVVVPTTILCSQHFSRFIERFKGFGLNIKQLSSVISSKEANIIRLELASGKINIIIGTHALLHKNTKFFNLKLLIIDEEQHFGVSQKEFLKSLKSSTHVLAMSATPIPRTLQMSMTGLKELSIIATPPLNRLEVLGLEQNYSYVTILDEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDAPDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILNSVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQLHLKMWPDLDMERERPMDRLLCGDVGYGKTEVAIRAAFKAIGDGKQVALLVPTTILAQQHYETIKERFQDYPINIGLLSRFRTRKEANETIKGLKNGTVDIVIGTHRLLSKDVVYKDLGLLIIDEEQRFGVTHKEKIKQHSLIPQDSKTEIIVNTKEFLQAIDRASLLAREGRNNVVKLSAKPAESIEISSNSPEIGKVVEAIVADQIEGEELNISFSPKYMLDALKVLEGAEIRVSFTGAMRPFLIRTPNDETIVQLILPV",
    "response#3": "MDQKLSKLVEELTTSGEPRLNPEKMKELKKICKSSEEQLSRAYRLLIAQLTQEHAEIRLSAFQIVEELFVRSHQFRMLVVSNFQEFLELTLGTDPAQPLPPPREAAQRLRQATTRAVEGWNEKFGEAYKKLALGYHFLRHNTPDQSKSIEEIKQDMEIERPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQHYETLIERMQDFPIEVQLISRFRTTKEVKETKEGLKSGFVDIVVGTHKLLGKDIQYKDLGLLIVDEEQRFGVRHKERIKIDYTIVVLAETETKKERIQSMLNEMHIPTFIDTPSRRNEGGSAIITEGSLSEGFELPYMQLVVVTEREMRAPVVPYGVDLHYWGQELPTAGKIVKSDSQHRFWKPSEVEEEVVNADISEMLRSRHITFAGKFEPVQHWCRAPRPDGRLCERQDRLKCPFHGKIVPRDDEGRPLTGSRLWSAQRAWVRRVLKGKSFAAIAPTGVGKTTFGVLMAVYLASRGEKSYIVVPTTPLVKMVEERALRLADAAGVPARIVALHSRLSQKQRRRLLERVAEGDFDILITTSQYLVRNAETLAGLREQWGRGFRFVFVDDVDAVLKSGRSVDAVLRVVGFSREEVELGWRLLQLQRRLVWETRRAQKELARRLERLRQQARRRGRRLTAEEVRELRRRVFEETLEPLYRELRRIEERAPVVPYGVDLHYWGQELPTAGKIVKSDSQHRFWKPSEVEEEVVNADISEMLRSRHITFAGKFEPVQHWCRAPRPDGRLCERQDRLKCPFHGKIVPRDDEGRPL"
  },
  {
    "reference": "MAVAGVAKRYAQAAFAVAKEHGQLDFWEQRLTDLEALARDSAVEEFIQNPAIPLEAKVQVIDRLFPGDENRYVRNLLVLLLERGRWHQLRDVVVAFRELLREHRGVIDVELVTAVPLEPSEVERLRRELAQRLSRPVELRTTVDPELLGGVVLKIGDEVFDASVRTQLTNLRRQLVGAAA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that It either transmits conformational changes from CF(0) to CF(1) or is implicated in proton conduction. The designed protein sequence is ",
    "response#1": "GNRAVL",
    "response#2": "GFPVL",
    "response#3": "GHPK"
  },
  {
    "reference": "MDYAVTAQQMKAIDQNTINGVGIPSMVLMERAALAVAEAAERMAAERKLGKKARILAVCGTGNNGADGVAAGRILQGRGYETVLLFAGNPEHDSEEMKAQKRIAANLNLAQSKVSEYREAPCDIILDAVFGIGLGREIGGEYRKVLEMLESMREQYGAGIIAVDTPSGIHGTTGEIMGIALEADVTVTFGYAKTGLLLYPGKQYAGKLVVADIGFPEASLLKSGWDAKLITPKDLTWLPRRRPEGNKGTFGKVLVIAGSRGMSGAAYFSALGAYRTGAGLVKILTVPENRQVLQTQLPEAIVSCYDPEDEEAMQNILEQECAWADVIVIGPGLGQESYTEYLLETVLSDAYVPVVLDADGLNCVAAHPYLTRYFTENIIITPHMGEMSRLTSIPIVDLKKDPLGEARAYSARYGTVCVLKDAVTVVTDKDGAAWINQSGCSAMAKGGSGDVLTGVIAGLLARGMECTEAAAFGVYVHGRAGQYAAAAYGENSVLATDVADSILKEL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be designed to have high stability and activity towards (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.2. The protein must exhibit NADPHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity, metal ion binding.3. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration4. The designed protein should have a high specificity for (6S)-NADPHX, AMP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MRKADASVIRSGTPGVTLMQNAGAAVADAAREMVSPGARIVVLAGPGQNGGDGIVAATRLREQGYRVSLGLLGKREAFSGDAALVAREWPSDVVAVDGANLAPAELVIDALFGGGLSREIEGAARAAIDRINMSGGPVLAVDLPSGIDGETGAVRGIAVRAARTVTFAARKPGHYLLPGRTFCGDVTVADIGVGSSDLDAEEAGDLLPVRDPMAHKKSVGQVLVIAGSRGMSGAAVLTGHAAYKMGAGLVAFAPPQGIAPVLNAALLEAIVWPQPETEAGTLFWDARSSLLELAGQYDVVALGPGLSTNPQTVEFVEKLVADIEKPLVIDADALNALAGDAGILKSREALTVITPHPGELARLYGVSSKDVLADWLGFARRAQERFGAVVVLKGASTVICSAEETTINVTGNPGLATAGTGDVLTGFIAALIAQGVDAYNAACLGVYLHGFSADIAAEDIGELSLMASDVIEYLPEALAWRDPDSYKGDNGEVLVVGGGPYAGAPTLSALAALRAGADLVRVACPGSVASEVQGFSPNLIVRPLPGDRLGPSHAERIASLAAGSDAVVLGPGLGDGDGTEKFVREFLTGYGGRAVVDADALGVVPEVDTDADLICTPHRGELVNMGGATVADPDERAELVREFAAELGHALLVKGSVDVISDGDEIRLNRTGNPGMTVGGTGDVLAGTVGALAAVTDPFRAAAVGAYAVGRAGDAAAGANGTGLVATDLPDRLPEAML",
    "response#2": "MRKADASVIRSGTPGVTLMQNAGAAVADAAREMVSPGARIVVLAGPGQNGGDGIVAATRLREQGYRVSLGLLGKREAFSGDAALVAREWPSDVVAVDGANLAPAELVIDALFGGGLSREIEGAARAAIDRINMSGGPVLAVDLPSGIDGETGAVRGIAVRAARTVTFAARKPGHYLLPGRTFCGDVTVADIGVGSSDLEESLGTDWLLNPDESHKGENGILVGIAGSVDYYGAPIYMSKGALRMGIDLLFLFSPEYIHNYISSSTHDIILKKSGERYIEFNNELKRIIEDKATAVALGCGIGKNEVSQESSTKIIEESLLVKKPIVIDADALDSVTLFDDLKGFAVITPHRGEFKRIFSIDPDENNVVKMAKKYNLVILLKGSVDIISDGEIVKKNKSFHHPSMTRGGTGDILTGAVGGLLSRGIDTFHSATLASFIVGSAGKMAFEEKSNSYFTSEVIDMIPQVFNPDESHKGENGILVGIAGSVDYYGAPIYMSKGALRMGIDLLFLFSPEYIHNYISSSTHDIILKKSGERYIEFNNELKRIIEDKATAVALGCGIGKNEVSQESSTKIIEESLLVKKPIVIDADALDSVTLFDDLKGFAVITPHRGEFKRIFSIDPDENNVVKMAKKYNLVILLKGSVDIISDGEIVKKNKSFHHPSMTRGGTGDILTGAVGGLLSRGIDTFHSATLASFIVGSAGKMAFEEKSNSYFTSEVIDMIPQVFDENWGKAALAPRRSTAHKNDFGHVLAVAGSEGKAGAATLVVLAALRAGAGLVTLAARPEVLRTALPGVPEAMGVPLPGNGPLGPGDLAPLRAALRGKTAFAMGPGIPRGPQTGQLLGELLAALDPGCATVVDADALNALAEERDAIAERLRKAPVRPVLTPHPGEFSRLTGEEVERVESDRIDAAVRASSRFGACVVLKGARTVVADPEGTSAVCAHGNPGMATAGAGDVLTGITAAVLARRSGGGSTGERARLAVFLHALAGDLAARRTGETGLVASDIAEVGLPRVFRR",
    "response#3": "MGDIESRIFAAGMPVAALMEKVGGQLAQRIQALYPNARTKRIGCLVGPGHNGGDALVVARELHSQGYDVTIYRPFGKLKDLTAQHAQYAERLGIPHCEQVEPLADCDVLVDGLFGFGLTRSLDGTIATIVDQINQWSTPVISIDVPSGLHTDSGAALGTAIRATRTLCLGLWKLAFMQDQALEYTGASELIDFGIPLADIAPALWREAIPRPGPSSHKYERGHVLVASGGATRTGAARLAARAALRIGAGVVTVASPPEALGVNAAQLTAIMLRGCDGPEGLHAILADERLNALVVGPALGVTAVTRAMVAVAAQARRRLVLDADALTSFEGLASELHQGFRSAPTVITPHDGEFTRLFKGHPDILDPSSKVERARRAAAYLSAVAVLKGADTVIAAPDGRAAINANGTPYLGTAGSGDVLCGIIAGLMAQHLPAFEAAAAAVWIHAEAGAAFGAGLISEDLPELIPEVLQGSHATNAFPLPRRARDAHKGNFGHVLVIGGDVGFAGAPLLAGEAAARVGCGLVTLATRPQHAGMQVAARPELMAWGVDRPAQLDPLLDRASVVAIGPGLGQSPWAAGLLARVLETNLPLVVDADALTLLARDPVQNERWVLTPHPGEAARLLDCSTAEIQGDRFTAVRELQSRYGGAIVLKGCGSLVATRKSGVTVCTDGNPGLASGGTGDVLTGVIAGLMAQGLEPESAALTGACLHGAAADRAVLEGERGMLAGDLMPWLRRLVNPG"
  },
  {
    "reference": "MWRNSEERENRTSGRSQGSYQEIIGRTWIFRGAHRGRVNKKNIVWHELIGLKVRVVNSTHPGYVGIEGYVIDETRNMLVIAGENKVWKVPKDVCIFEFETWDGTKIKISGEKLVGRPEMRLKKRWRK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends2. We need a protein that can perform Endonucleolytic cleavage of RNA, removing 5'-extranucleotides   from tRNA precursor. with high efficiency.3. The ideal protein would be one that plays a critical role in tRNA 5'-leader removal through ribonuclease P RNA binding within ribonuclease P complex, cytoplasm, ribonuclease MRP complex. The designed protein sequence is ",
    "response#1": "MVNPNP",
    "response#2": "MF",
    "response#3": "MPEIKFVRG"
  },
  {
    "reference": "MEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEEDAESEDEDEEDVKLLGMSGKRSAPGGGNKVPQKKVKLDEDDDEDDEDDEDDEDDDDDDFDEEETEEKVPVKKSVRDTPAKNAQKSNQNGKDLKPSTPRSKGQESFKKQEKTPKTPKGPSSVEDIKAKMQASIEKGGSLPKVEAKFINYVKNCFRMTDQEAIQDLWQWRKSL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF; Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. The designed protein sequence is ",
    "response#1": "LFGCELKADRDYHFKVDNDENEHQLSLRTVSLGAGAKDELHVVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLC",
    "response#2": "LFGCELKADRDYHFKVDNDENEHQLSLRTVSLGAGAKDELHVVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLC",
    "response#3": "LFGCELKADRDYHFKVDNDENEHQLSLRTVSLGAGAKDELHVVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLI"
  },
  {
    "reference": "MGARTGPPRAADADAGRPPLSVLERETTKDVEDVMTELSRSEDKRLVLATGRAHPELAQEIAEELGTELLPMSAYDFANGEIYVRSGESVRGKDVFIIQSHPAPLNNHLMEQLIMVDSMKRASARRITVVSPFYPYARQDKKGRGREPISARLVADLYKTAGASRVMSVDLHTAQIQGFFDGPVDHLFAIPLLADHIRSSVEGEEVTVVSPDTGRVRVAEQWADRLGGVPLGFVHKSRDLTVPNKAESKTVVGDVEGRVCVLIDDMIDTGGTIAGAVQILKDAGAKDVIIAATHAVFSDPAAQRLGECGAREVVVTNTLPIPEEKRFETLTVLSIAPLIARAIREVFEDGSVTSLFDGVA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should interact with enzymes and substrates within the 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, Metabolic intermediate biosynthesis, 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1 pathway to enhance or regulate its activity.2. The designed protein should have ribose phosphate diphosphokinase activity, ATP binding, magnesium ion binding, kinase activity within cytoplasm to regulate nucleotide biosynthetic process, ribonucleoside monophosphate biosynthetic process, 5-phosphoribose 1-diphosphate biosynthetic process, phosphorylation.3. The active site of the protein must be engineered to enable effective catalysis of ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-   diphosphate + AMP + H(+).4. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.5. The presence of Ribose-phosphate pyrophosphokinase N-terminal domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGVDRVLTIDLHAGQIQGFFNIPVDNAAPTLASYFRRKKLENVVVVSPDHGGVTRARVFASFFNAPLAIIDKRRPEPNKAEVMNIIGDVKGATCIMIDDIIDTGGTLMAGANALKEAGAKEVYAAATHGVLTSNATERLQNSVINEIVITDTIPLPPEKQLPGMVQLSVAPLMAAAITAIFNNGSVTSLFGDAAV",
    "response#2": "MKIFAGNATPKLANLIAKRLYTQLGEATVSRFSDGEISVQINENVRGDDIFIIQSTCAPTNDNLIELVVMIDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKIVADFLSSVGADRDLHAAQIQGFFDVPVDHLFAAPVMLAPLRELGENNIVIVSPDAGGVERARAYAKRLNAPLAIVDKRRDRPNQATAVHVIGEVEGRVAIIVDDMIDTAGTLCAGAEVLLKYGAEKIVACAAHPVLSGPAIDEAARLVGPRIRTAALAEGSVTVRLDDLARSPILLEDMFELNLENPIVVSPDIGGVIRARAIAKLLNDADMAIIDKRRPRANISQVMHVIGDVLGRECVLVDDIIDTGSTLCKAAEALKEHGAKRVFAYATHPVFSGNAMDNLRKSMIDEVVACDTIDLPAALSPVILDYLLQKELEDFVVVSPDVGGVARARAFAKKLNDAPLAIIDKRRQAHNVAEVMNVIGDVKGKNAILVDDMIDTAGTLLEGARLLKKEGAKKIYACATHAVFSGPAISRLSSDVFEEVIVTNTLPIPAEKQFDRFRMLSVAPLVGAAIRTVFDDGSVTSMFDGAA",
    "response#3": "MKIFSGTSNQPLAEAICKYIGIELGKCDVTRFPDGETFVKILENVRGQDAFIVQSTSPPTNHHLMELFIMMDALRRASASRITAVLPFYGYARQDRKDQPRVPITAKLVANLLVAAGINRVITVDLHAGQIQGFFNCPVDNAAPTLASYFRRKKLENVVVVSPDHGGVTRARVFASFFNAPLAIIDKRRPEPNKAEVMNIIGDVKGATCIMIDDIIDTGGTLMAGANALKEAGAKEVYAAATHGVLTSNATERLQNSVINEIVITDTVELGENVQKISRVSIAPLLAKSIREVFEDGSVTSLFN"
  },
  {
    "reference": "METTVVVLGHVDHGKSTLVGRLLYDTGQVQDDRVDFACRRSAEQGRSLEFAFLLDGLAEEQEQGITIDFTQTRLKFDDRDFVIADAPGHREFLKNMISGASRAEAAIVVIDASEGVREQSRRHGYLLSLLGVTQIAVVINKMDLAGWSEAVFQSIRQEYADFLHSHGMKAMQYIPVAAYTGDNLIQPSKNLAWYQGPTVREQFLAFQPVRDAQEALRFVVQDVYRFGSRRLLAGRIEAGCLTAGQKVVVWPTAEKTKVLRIDCWPRLETRSASQGDCVALELADPLFVERGMLLAGTEQPLTSSRCFYARVVWLGRKELVSGQRYKLKLGCQETGAWFERFDRVIDIGNLQDASLAGIPAGFVGEGLLMTDQPIVFDAFSAIPATGRFVLVDGYQIAGGGIVLAAAAGERQDFARFSHEKQAAETNPALFAEASRISRLERRERNRHKSFAVWLTGLSGAGKSTLARGLEEKLFRAGVQTYVLDGDNIRGGLNQDLSFSAGGRRENIRRVGEVAKLFVDAGFVVITAFISPYANDRGHARELLASGEFVEVFVKCSLAACEQRDVKGLYAKARQGIVRQFTGIDDVYEVPEQPELVIDTEQYSVEECVEQLVQYVIANCRLAESAES",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Catalyzes the synthesis of activated sulfate.2. The designed protein should have GTPase activity, ATP binding, GTP binding to facilitate hydrogen sulfide biosynthetic process, sulfate assimilation, phosphorylation.3. The design of the protein must focus on the precise arrangement of amino acids in the Phosphoserine intermediate to enable effective catalysis of adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate   + ADP + H(+).4. The designed protein have Tr-type G domains with specific sequence motifs that are crucial for its activity.5. The protein should have a high affinity for ATP.6. We require a protein that can specifically bind to the metabolites produced in the Sulfur metabolism, hydrogen sulfide biosynthesis, sulfite from sulfate: step 2/3 pathway. The designed protein sequence is ",
    "response#1": "KEMLRFLTCGSVDDGKSTLIGRLLHDTKMIYEDQLAAIKRDSVKHGTTGGEVDLALLTDGLKAEREQGITIDVAYRFFSTDKRKFIIADTPGHEQYTRNMATGASMCQLAIILVDARHGILPQTRRHSFICSLLGISHLVVAINKMDLAGYSQERFNEICRAYSDFAAKLDIRDVHFIPISALKGDNVVELSANMPWYHGVTLLNHLETVHIAADRNFRFPVQLVQRPNLDFRGFAGTIASGVVKKGDAILVLPSGKKSRVKSLVTFEGELAEAWCPMPITITLTDEIDISRGDMLVRAGNTPEFDATVCWMSDDAALEPGRDYIIKHTTRTTRARVSDLDYRLDVNTLHRDKSATALKLNELGRVTLRTQQPLLLDEYSRNSVTGSFILIDPDTNGTVAAGMIRFALRRSQNVHWQALEITREARAEQKGQRPRVLWFTGLSGAGKSTVANLVEKRLHALGHHTYLLDGDNVRHGLNKDLGFTDTDRVENIRRVAEVSKLMVDAGLIVLVSFISPFRAERQMARDMMGEGEFVEIFVDTPLEVAEERDVKGLYKKARAGEIKNFTGIDSPYEAPEKPEVHLATTAKTPEEAAGIILEQLGA",
    "response#2": "TMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLEALQSDSKKVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFNTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLVQTRRHSYLAHLIGIKNIVLAINKMDLVDYDQSVYDAILKDYTEFATSIGITAFTPIPISGFKGDNITTLSHFGHDFKATIVWLNEKPLDVTAGYLIKQTTRTCPGEITLHHGIDVNTLEEKPLPTLHLNEVGVCSISLTQPIAFDNYRKNHATGSFIIIDRLTSNTVGMGMFRLPVQWVNRPNLDFRGFSGTIEAGSIKPGDEIAVPASGQTSIVDKIVTMDGDLDEAVAGQAVTVTLKDEIDISRGDMLVRPADQFEGEVIWMHDEALLPGRSYLIKFGTQSAAAQISELKYKVNVNTLEHAAAKTLDLNEVGICNIVLDRNVAFDPYADSQGTGRFILIDRYSNATVGVGMIRHALRRAQNVHWQAIEIDKEHHARMKNQKPCVIWFTGLSGAGKSTIANLVEKKLHVAGRHTYLLDGDNVRHGLNKDLGFTEADRVENIRRVGEVAKLMTDAGLIVITAFISPFRAEREMVRERMPEGEFVEVFVDTPLEVAEERDVKGLYRKARAGEIKNFTGLDSPYEEPSAPEITVNTVEMSVEEAADYIIEQLLAREIMG",
    "response#3": "DMLRFLTCGSVDDGKSTLIGRLLHDSKMIYEDQLAAITKDSKKSGTTGDKVDLALLVDGLASEREQGITIDVAYRYFSTDKRKFIIADTPGHEQYTRNMVTGASTCDLAIILVDARAGVKTQTRRHSFLVSLLGIKHVIVAINKMDLMEFSEEVYENIKKDYLVFSEQLDIPDIQFVPISALDGDNVVNKSENTPWFEGSFRLLVQWVNRPNSDFRGFSGPIVSGVIRKGDAVQVLPSGRQTRVARIVTYSGEVDEAVARQSVTLVLQDEIDVSRGDVLALAQAADQFEATIVWMADEEMLPGRPYWLKIGAQTVSATVQHPKYQVNVNTMEHLAAKTLDLNAIGVANLSTDKPIVFEPYEANRALGGFILIDKITNATVAAGALTFALRRATNIHWQPLDVDKAARARLKQQKPCVLWFTGLSGSGKSTIANLVEKKLHALGQHTYLLDGDNVRHGLNKDLGFTEADRVENIRRVGEVAKLMTDAGLIVLTAFISPFRAEREMVRAMLPEGEFVEIFVDTPLEVAESRDVKGLYKKARAGELKNFTGIDSPYEAPEAPEIRVNTVEMSAEEAADRIVAHLIP"
  },
  {
    "reference": "MAKVESFTLDHTKVKAPYVRLITVEEGPKGDKISNYDLRLVQPNENAIPTAGLHTIEHLLAGLLRDRLGGVIDCSPFGCRTGFHLITWGEHSTTEVAKALKSSLEEIAYKTKWEDVQGTTIESCGNYRDHSLFSAKEWSKKILDEGISDKPFERHVVD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should incorporate a binding pocket that specifically recognizes Fe cation to enhance enzymatic efficiency.2. The protein should be able to modulate quorum sensing in a way that leads to a desirable outcome.3. The protein must exhibit the following characteristics: Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment; Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD).; The regulation of gene expression in response to changes in cell density is called quorum sensing.4. The protein should be designed to have high stability and activity towards S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = (S)-4,5-   dihydroxypentane-2,3-dione + L-homocysteine. The designed protein sequence is ",
    "response#1": "MPIPLDIDILHCAESTFKAGHLIAPMMAKREEPYQYIYTDEKRAEELAGAEEITYPDGRRVPLRTILGRPDGSEPVALYFHGGGFIMADLDSYDGFCRELSRRTGAVVVSVDYRLAPEHPFPAAPDDCYAALCWAAANAGELGGDGSRLAVGGDSAGAGLAIVTALKARDEGGPAIAYQMLFYPVTDAARDSASHRAYADGYSLTAADMEWFYRCYAPGLHDRTHPSVSPLRAEDLRGLAPALVTTAEYDPLRDEGEAYGARLKAAGVPVEHRRFAGMIHGFLNFSALVPEADRFVEEAAGVLRKALD",
    "response#2": "MGYNHPTPLSSEFLALPKIREQFEKIGLLFKRTEWEAXEXSXPTSDLVEFYTKXGWITXDXKXTXXXIPXGXYDKAFIXVFNKLXEFYXAXGHVXDXRXFXGNXYXPNXSXRXKXGSXXXKXKXXLAXRXXZFXXXHXXXHXXQXXVXLXWXXXTX",
    "response#3": "MTSKTHFGFETVKESEKEAKVAGVFSSVAAKXDXMAXLVXXKXWXXFXSQXVXVPXXKXIIXLAXGTGXXXVKWXXXTXXGXGXXXDXNXAMXQXNQGXGXXVXNXXGXXXXXXXXXXXXXXXXXXXXXXXXGXXVXXXGXXXGXXANWXGXXXXXAQXRXAVXGXXN"
  },
  {
    "reference": "MYNVKLEVFEGPFDLLFHLIEKNEIDLMDIPISVILDQYMEYIKTLQEMDLNVASEFIVMAATLLEIKSKMLLPKQKFEGQQLEMEEVDPREELVTKLIEYKKYKIIAQTFKEMCKIGSRFFREEPEVKYIDKKIAFNYSSEDIYKAYLKVISKNNAKEDEIEIKKDEYTVESKIKELLVKLVKKTVLWFSEFIKKSRAKGEIIVSFIAVLELVRLNKIIAEQKTTYGDILIKSFGRGEKNEQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Participates in chromosomal partition during cell division; May act via the formation of a condensin-like complex containing Smc and ScpB that pull DNA away from mid-cell into both cell halves.2. The designed protein must be able to regulate chromosome segregation. The designed protein sequence is ",
    "response#1": "EAGGUG",
    "response#2": "SALGF",
    "response#3": "MGQ"
  },
  {
    "reference": "MAFVLKYAKTVRNNWKKSVVCVAAAAYGVKYAKEKYDQEWIRREFCAEALKYGNAKIGLQERPRKITVFLNPAASGGGAVKVFEKNAAPILHCAGLEVNIVKVEYEGQVKKFLSILERSDTDGIVVAGGDGTLLETITGIMRKEDKTFRQNIPVGVIPLGHKNRFAKLLFGSDNDQVLLMCQCAMSVVRGLGRSVDVISLKEDNGRTAYALCGLEMGAFREAEQRKSKYWYFGGLKHRMTYLRSAFLDWPPVLKAKLSYVLATEENTQPRQQTEQPKEQPKGSWLSMLFKPKVIKPKAELFEEETDDDNDVVETRNLSTVELTTVSTCLQDPGQTVKGLELGIGPAEPSIWEFISEGWRRVSEPTLRLGTRSNETILVKKIRIEPEDPTKFYNIDGELFESVPVTIELLRKRVKFFCVDPDFKIS",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a specific function in glycerolipid metabolic process and should be localized to mitochondrial inner membrane, mitochondrial intermembrane space in order to carry out this function efficiently.2. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.3. A protein that can modulate the overall flux of metabolites through the glycerolipid metabolism, Lipid metabolism pathway, enhancing or suppressing its activity as needed.4. The protein should have a low Km value for an N-acylsphing-4-enine + ATP = ADP + an N-acylsphing-4-enine   1-phosphate + H(+).5. The presence of DAGKc domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MIGLTAGAYSEAEKKKKKYWYFGPLKFKWAYIRSTLTQWPSVISGKISYIPATEENTKLTKIVIKQPVPEVRRSWWNILYLFYKPRPQHSQEVYEEVEAEDGQDEETISEDVSSMELSVIAPIAQGQQEKIVAPELHIGPSQINRQDFISEGFRREKLPTRVFNSPDDRHSFVKKFKFVPDLPEDAEKKVWYSIDGEQYEAVPVEIKLLRNRLNIFQSP",
    "response#2": "LTDMAWGSYRDTFVKESKYWYFGPIKTFMTHFFSLFKTDIRTPRLFQLAYNPPKPIPKDIEKKTEEAKLMAVNSLISKLLIIEVEEPLAEEKYDAPIEEYGSIEFMASVNNHDPHDKREKAGARIVIESSDFNWLNFLREGPKRIVAGKPIPVKPETVIDCSEFQLVPLSSNESWFGMDNEKFEAFSCHATVIPL",
    "response#3": "LAGLEWGAFRDTNARRDKYWYFGGLRGYAAYIFAGFKGEKTITWNVQANMEYSPPCNGCSNCYEQRLDLHPDQAQSKSWWSAFGKRQPGSVVPTVDYRGKVNEDCRTVRKLDVATSDFSLISSNVSKEHNKVPQLQVSFGPEHVSYNDFVKEGWRRENGEDPSRPCKILAKEVKLWPVQAVKHQDNATASSTQDNIPTGPKDIEKWISIDNEDYEVKAVHATLLPNLVKMFYSPG"
  },
  {
    "reference": "MQQNKKLGQCFLKDKNIVKKAINAANINKNDIVLEIGLGKGILTKELAKQCKKVIVIELDKKLEIFWEDIIKEYPNVEIIWNDALKVNLKELGFNKVVANLPYQISSPITFKLLDCDFEVAVLMYQYEFAKRMGAPSGTKEYSRLSVSVQYRAVVDYVCKVSPSAFSPKPKVDSAIVKITKKNEPIHNIDNWEFFDGFNRALFQHRNKNTKKALIHSAHEINMDRDKLKELLNSNEIMEFNDLLGKKVYTLSLKEIGVLCNNLYKIINKSK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein localizes to the cytoplasm. The designed protein sequence is ",
    "response#1": "RRRRRGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS",
    "response#2": "RRRRRGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS",
    "response#3": "RRRRRGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS"
  },
  {
    "reference": "MGSAPAQIPTSFGHELRACLRCRLVKTYDQFRDAGCENCPFFKMEEDHERIVEVTTPNFNGIISVMDPSRSWAARWLRIGKFAPGCYTLAVSEPLPEEMQHLCQEERVQYVLPKRM",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that May enhance transcriptional pausing at sites proximal to the promoter, which may in turn facilitate the assembly of an elongation competent RNA polymerase II complex .; May regulate transcription elongation by RNA polymerase II2. A protein that can perform RNA polymerase II complex binding in DSIF complex to support regulation of transcription elongation by RNA polymerase II, chromatin organization, positive regulation of DNA-templated transcription, elongation. The designed protein sequence is ",
    "response#1": "GLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTSIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIG",
    "response#2": "GLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTSGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDT",
    "response#3": "PSRTPNYDSSSKTPAYPESEHSARTPAYNNDYDIPLSPAYEPDAPEAYDNAPARTPAFVSRTPGYDTYENSSPTYEPDAATKVEEDIGDTSSPTYDSPPHSYVVPTPGAMLNPATPGAYHVDTPGFAAPMTPGSGGAYDQYVAPSC"
  },
  {
    "reference": "MHPLKSKNILIGVSGGIAAYKIPILIRXFKEIGSXVKVILTEAAKDFVTPLTLEVXSQNPVLSTFVSEEFDNPKWNNHVELSSWADLLIIAPATSNIISSMVNASCNNLLLAVYLSSKCPVFIAPAMDLDMYKHPANKKNISKLIKYGNNILPVGEGYLASGLCGXGRMMEPELILQNIASFFXKKKPLLNKKILITAGPTYEPIDPVRFIGNKSSGKMGYELAREARNLGANVKLIIGPNSLDMKSFDGELINILTADEMFNKALLHFKNSDIFISAAAVSDFKPTFMEIKKMKKNTTFRNIELEPTKDILNELSKIKTNQILIGFALETNNEYENAKRKLKNKKLDAIILNSLNDESTGFDFDTNKITYIKKNGESIKFDLKTKKNVAKDIFKEIIKIKE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design must be able to perform phosphopantothenate--cysteine ligase activity in order to contribute to pantothenate catabolic process.2. Mg(2+) is required for the proper folding and stability of the protein.3. The designed protein should interact with enzymes and substrates within the CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "LEGRKVLVTAGPTIEDIDPVRYISNRSSGKMGFAIAHAARNRGAQVVLVSGPTELDFPNTIRVRTTDEMRRAVLQNVADADAVIKAAAPLDFRPKSVAAQKIKKKTANLVLDLEPTVDILKELGGHKNGKVLVGFAAETENHLKHGLEKLKAKNLDLIVINSASGPDSAFDSDMNHATILFKGEQLVELEKMTKEELAERLLQQ",
    "response#2": "MNILLGITAGIAAYKTPQLVRLLTKKGHTVRVIMTERAQDFVTPLTLSTLSKQPVLTDFQSADHQWNSHVEAALWAEALLIAPATANTMAKMANGICDNLLLATYFSAKAPVFIAPAMDLDMYAHPTVQQNIKRLESFGNYIIPAAYGELASGLIGQGRMEEPENIVAFLEEHILGRLLEGRKVLVTAGPTIEDIDPVRYISNRSSGKMGFAIAHAARNRGAQVVLVSGPTELDFPNTIRVRTTDEMRRAVLQNVADADAVIKAAAPLDFRPKSVAAQKIKKKTANLVLDLEPTVDILKELGGHKNGKVLVGFAAETENHLKHGLEKLKAKNLDLIVINSASGPDSAFDSDMNHATIEVFWKDGKRELPRADKLTLARHLIEQIAQHYQK",
    "response#3": "KKILLGITGSISAYKAADWVRALRKEGGTVTVVMTESATKFISPLTMAALSGNRVYHHMFKDDEPEKIPHINLAREHDLNLIAPATAQTIGRLAHGLTDDLLSAIILASSAPTVVCPAMNSKMYLHPATQTNIATLKTYGYHLVDPDNGTMACGEEGPGRLTEGAAEDPLAGVSVVITAGPTRESIDPVRYISNHSSGKMGYAIAQAFAERGAEVTLISGPVNLPTPANVCRIDVISAQEMWQVAMESAVKNQLFIGCAAVADYRMAEISDQKIKKTSDNDELTLKLVKNPDIIASVAALTENRPFVVGFAAETHNVEQYAKDKLRRKNLDMICANSVAGGAVFGEDKNALHLYWRDGRVELPRADKQVLARQLLDEAARIHR"
  },
  {
    "reference": "MALRNVPFRSEVLGWDPDSLADYFKKLNYKDCEKAVKKYHIDGARFLNLTENDIQKFPKLRVPILSKLSQEINKNEERRSIFTRKPQVPRFPEETESHEEDNGGWSSFEEDDYESPNDDQDGEDDGDYESPNEEEEAPVEDDADYEPPPSNDEEALQNSILPAKPFPNSNSMYIDRPPSGKTPQQPPVPPQRPMAALPPPPAGRNHSPLPPPQTNHEEPSRSRNHKTAKLPAPSIDRSTKPPLDRSLAPFDREPFTLGKKPPFSDKPSIPAGRSLGEHLPKIQKPPLPPTTERHERSSPLPGKKPPVPKHGWGPDRRENDEDDVHQRPLPQPALLPMSSNTFPSRSTKPSPMNPLPSSHMPGAFSESNSSFPQSASLPPYFSQGPSNRPPIRAEGRNFPLPLPNKPRPPSPAEEENSLNEEWYVSYITRPEAEAALRKINQDGTFLVRDSSKKTTTNPYVLMVLYKDKVYNIQIRYQKESQVYLLGTGLRGKEDFLSVSDIIDYFRKMPLLLIDGKNRGSRYQCTLTHAAGYP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should contain one or more SH2 domains that are essential for its function.2. The protein design should emphasize Acidic residues, Polar residues, Pro residues compositional bias.3. The protein should be designed to localize specifically to TCR signalosome, cytosol, cell-cell junction in order to perform its role in positive regulation of protein kinase activity, mast cell activation, transmembrane receptor protein tyrosine kinase signaling pathway, intracellular signal transduction more effectively.4. The engineered protein is expected to adopt a strand-helix-turn-helix-helix-helix-helix-turn-helix-helix-based conformation. The designed protein sequence is ",
    "response#1": "CAPYSGKVCKEYLTGQVWYSLEDPTGGWKNEQVTTALWDELISDLTGLCREAAEKMLCAYAFPNCHMEGGRAVKAPLCFEDCQATHLQFCYNDWVLIEEKKERNMFIKSRGHFRLPNCSSLPHC",
    "response#2": "QKDGDKITSWFLSKGQGKTRDDSADYIPHDIYVIGTQEDPLGEKEWLEILRQSLQEITSVTFKTVAIHTLWNIRMVVLAKPEHENRISHICTDTVKTGIANTLGNKGAVGVSFMFNGTSLGFVNSHLTSGSEKKLRRNQNYMNILRFLALGDKKLSPFNITHRFTHLFWLGDLNYRVDLPTWEAETIIQKIKQQQYADLLSHDQLLTERKEQKVFLHFEEEEITFAPTYRFERLTRDKYAYTKQKATGMKYNLPSWCDRVLWKSYPLVHVVCQSYGSTSDIMTSDHSPVFYSRNTELRRVEDNDIYRLAKILDENSCWRKLMSIIPKGMDVQACSGAGCLNFPAEIKKGFKYTAQDVFQIDEAANRLPPDQSKSQMMIDEWKTSGKLNERPTVGVLLQLLVQAELFSAADFVALDFLNESTPARPVDGPGALISLC",
    "response#3": "PQVVILSLILHLADSVAGSVKVGGEAGPSVTLPCHYSGAVTSMCWNRGSCSLFTCQNGIVWTNGTHVTYRKDTRYKLLGDLSRRDVSLTIENTAVSDSGVYCCRVEHRGWFNDMKITVV"
  },
  {
    "reference": "MSEFKVKTGLAQMLKGGVIMDVVTPEQAIIAERAGACAVMALERIPADMRKSGQVCRMSDPRMIKEIMEAVSIPVMAKVRIGHFVEAQILEELQVDYIDESEVLTPADWTHHIEKHNFKVPFVCGAKDLGEALRRINEGAAMIRTKGEAGTGDVSEAVKHITKIKAEIQQYKENLKTESDFAAKATELRVPVDLLKTTLSEGKLPVVNFAAGGVATPADAALLMQLGCEGVFVGSGIFKSSDPEKLACAIVEATTHYDNPAKLLQISSDLGDLMGGISIQSINEAGGKNGARLSEIGW",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme- catalyzed isomerization of RBP and G3P, respectively.; The ammonia is provided by a SNO isoform2. The designed protein must have a Schiff-base intermediate with D-ribose 5-phosphate active site that is highly conserved among related enzymes.3. The protein design must be focused on enhancing the efficiency of amino acid metabolic process.4. The protein should be optimized for activity in Cofactor biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "LIGEALVGDGAELAHIDLIMGNKEGAVGQAFANSISQLSKGHTPLLAVVRPNLPTKPSTLIIPKVTLKKEYQVNQMFGPVQAAVAKAVADSIEEGVFEGVDIEDTVIMASVFVHPTAQDYNKIYRFNYGAMKLALRRALDRFPDVETLLHEKDRAAHAV",
    "response#2": "LIGEALVGDGAELAHIDLIMGNKEGAVGQAFANSISQLSKGHTPLLAVVRPNLPTKPSTLIIPKVTLKKEYQVNQMFGPVQAAVAKAVADSIEEGVFEGVDIEDTVIMASVFVHPTAQDYNKIYRFNYGAMKLALRRALDRFPDVETLLHEKDRAAHAA",
    "response#3": "IGEALVGDGAELAHIDLIMGNKEGAVGQAFANSISQLSKGHTPLLAVVRPNLPTKPSTLIIPKVTLKKEYQVNQMFGPVQAAVAKAVADSIEEGVFEGVDIEDTVIMASVFVHPTAQDYNKIYRFNYGAMKLALRRALDRFPDVETLLHEKDRAAHAVR"
  },
  {
    "reference": "MPLINQNNKVGALLSPWIAAATLPADLADIELTGMTLDSRQVRSGEMFVAVNGHAVDGRRFIPAALESGAAVILAEADDIAENATLVRQDGIFIVYLYELGHLLSAIARRFYGQPDEQLKLVAITGTNGKTTISQLLAQWAEQLDYCAGVMGTTGNGLLQQLKPAVNTTGSAIEIQQVLAGLVNDGADFAAMEVSSHGLVQGRVKALDFVASIFTNLSRDHLDYHGDMTAYAAAKKTLFTNHQSGTAVINADDEVGREWLASLPDAVAVASEQSNLSQHSGRALWLEQVEYSTQGVTVVFDSSWGGGKFTAPLVGSFNVMNLMLALATLLATGHVLSRLIETAPRLQAVIGRMEVFQKPDKPMMVVDYAHTPDALEKALQALRVHCRGKLWCIFGCGGDRDNGKRPMMAGIAERLADKIILTDDNPRSEDPAVIVADMLAGLQQPDLAVVNHDRYAACQWALTSADADDIVLVAGKGHEDYQILADRTIHYSDRETVGELLENNA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein should be able to manipulate the activity of enzymes in the peptidoglycan biosynthesis pathway, either by activating or inhibiting their function.2. The protein must be able to magnesium ion binding.3. The UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding site should be located in a region of the protein that is accessible to the ligand.4. The designed protein have Mur ligase C-terminal domains with specific sequence motifs that are crucial for its activity.5. The protein should be designed to have high stability and activity towards ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "MKLRDLIPHVGFSCEGDPEITSIEMDHRQVKKGSLFVCVKGYTVDGHDFATLAVESGAAAIIAERPVDVSVPVIIVKNSKRAMAVLADGFYGHPSGRLNMIGITGTKGKTTTTYLVKSILENAGMKAGLMGTVETRIGDKILPSEKTTLTPESLDLQALFAEMVKEGCEYCVMEVSSHALALGRVNGISFDTAIFTNLTQDHLDFHKTMENYFNAKAKLFEGLGRNKKAVVNIDDPYGRRLIEMSGSDIITYGIEKNADVRAENISMNINGTSFTVSTPVGSFPVKLKLVGKFNIYNVLGAIGAALVEGISLEEIKSGIENIESVPGRIEMIKVSDQFTLLIDYAHNAMALESLLTTLREYEPHRLISLFGCGGNRSRQRRFEMGEVSGKLADLTIITSDNPRFEEPQDIIDDIKTGMAKTDGNYIEICDRKEAITYAIEHGEPGDIIVLAGKGHETYQILKDRTIHFDDREVAREAIRGRK",
    "response#2": "MKLSELLQGLPPVEITEPAQDVRVDSRQVSTGDIFVAVPGVNEDGARFIPAAVAAGASIVVCRPGGAEEAAALAGGCRVVHHSDPREALWRLAELYGHPSRQLQVVGITGTNGKTTTSYLIESIMAAAGIATAVLGTVSYRLGATVLPASHTTPESTELQEVIRQLVDRGAAGVVMEVSSHALEQRRVDGCQFDVGVFTNLTRDHLDYHGSMEQYLEAKLRLFREFLQPSPNKPNRRAVVNLDDEYGEVVARESVCPVITYGIAKGDVRAVDVHSSISGITGKVVTPSGTIEFSSGLVGRFNLYNILAALATAVAAGLPLAEALTRLAHLKGVPGRMQLVATRDNGASVFVDFAHTPDAISTALQALRPHVLGRLIAIVGAGGDRDATKRPLMGRAASEHADLVIVTDDNPRSEDPEIIRSEVLLGAPDATEIGDRAEAILRGVDALGPGDALLICGKGHEDYQILGTEKRHFDDREEAAQALQRRLS",
    "response#3": "MSLRDLLSVTGVASDSRLAGPGDLYLALPGDRTHGARFAAQAVRAGAVAVLTDAAGAALLEGDGSLGPDVPVVVVDRPRALMAGLAAEVYGRPSDGLTVVGITGTKGKTTTTYLIKSILEKAGYHVGLVGTIETIIRDKHIPANNTTPESFLLQQYFKEMEEAGCEIVVMEVASQGLKLHRTQGFTFEIGIFTNLEPDHIGPNEHADFEEYLACKGLLFKQCRLGIVNRDDAHVDALLKGHTCELETYGFDKAADIHAENLQLVNKPGELGIAFEVKRKEKEESFRVEVPAPGRFSVYNALAAIAVAVEAGLDPDTVAAGVASCPGVPGRVEPVAISSKFSMMIDYAHNAMSLESLLTTLKAYEPKRLVCLFGCGGNRSKLRRYEMGEISSNLADLTVVTSDNPRFEEPEDILNDIITGVKKGKGEYVSIIDRKEAIKYCIENAKEGDVVVLAGKGHETYQEIAGERVPFDDRQVAREAIARAREGRC"
  },
  {
    "reference": "MDRRTPYTLSVLAPSTDGAEESRTQIQARLREFVLEFQLDNAFIYRDQLRQNVLVKQYFCDIDIAHLISYNEELAHKLTTEPADIIPLFEAALQQCTQRIVYPSQRDIVLPSHQLLLHSSASHISIRDLNATNISHLVRIPGIVIGASTISSKATVVHIRCKGCDHSENIRVEGGFSGLTLPRRCGRQKLPGEEPGEQCPLDPYVIAHEKCQFVDQQVLKLQEAPDQVPVGELPRHVLISADRYLANRVVPGSRCTVMGIFSIYSKGGKKDGAVAIRNPYLRAVGISTDLDHTAKGNAIFTEEEEQEFLELSRREDLYEALARSIAPSIWGNLDIKKAIVCLLMGGSKKILPDGMKLRGDINVLLLGDPGTAKSQLLKFTEKVSPIAIYTSGKGSSAAGLTASVQRDPQTREFYLEGGAMVLADGGVVCIDEFDKMRDEDRVAIHEAMEQQTISIAKAGITTILNSRTSVLAAANPIFGRYDDLKTPGENIDFQTTILSRFDMIFIVRDDHDRNRDENIARHVMGVHMGGRGIEEQVEAEIPLEKMKRYISYCRSRCAPRLSPEAAEKLSSHFVSIRKQVHRAEMDANTRSSIPITVRQLEAIVRITESLAKLSLSPIATEAHVDEAIRLFLASTMDAITQGEGQGSKELMEEVSKIEDELKRRLPIGWSTSLATLRREFVDGRGYTEQALNRALVILQRRDTIQIRSGGSQVYRHGV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that can perform ATP hydrolysis activity in MCM complex, chromosome, telomeric region, cytoplasm to support regulation of DNA-templated DNA replication initiation, subtelomeric heterochromatin formation.2. The protein must exhibit the following characteristics: Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells; The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit.3. The presence of MCM domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MYEYLSRSIAPEIYGHLDIKKALLLLLIGGVTKEMGDGMHIRGDINICLMGDPGVAKSQLLKYIAKVAPRGVYTTGRGSSGVGLTAAVMRDPVTDEMVLEGGALVLADNGICCIDEFDKMDDSDRTAIHEVMEQQTISISKAGITTTLNARTSILAAANPLYGRYNPRVSPVENINLPAALLSRFDVMFLILDTPSRESDEELANHEFLTSYITYARNNINPVISEEAAKELVNAYVGMRKLGEKN",
    "response#2": "QEQFGDFLEGFVEERDPHGAPPSSAVTTDKYYVAQIHGMRTYQLSTLYVDFRHLGQWQNGSLSDAVATNYYRFLPFITAALHGAIAKHEPQYFREHRQPTASSNQTSSAASNAASASQSELGSKTSNQQTDKLFAIAFYTKSKVKYMNVLQSVADRKSSQVLVDLDDLVEYEKSLDSEDASALNLVSSIETNAYHYIEILSRAVDSLLPTPEKEPTKRTLSSSLLNRMVSIEGIVTRCSLIRPKVVKSVHYNEVKDKFHFREYRDQTMTNGVTTSSVYPHEDEEGNPLMTEYGFCTYRDHQTISIQEMPERAPAGQLPRGVDVILDDDLVDKVKPGDRIQLVGIFRTRTLSSSLLNRMVSIEGIVTRCSLIRPKVVKSVHYNEVKDKFHFREYRDQTMTNGVTTSSVYPHEDEEGNPLMTEYGFCTYRDHQTISIQEMPERAPAGQLPRGVDVILDDDLVDKVKPGDRIQLVGIFRTPDIRLGITKSKVKYMNVLQSVADRKSSQVLVDLDDLVEYEKSLDSEDASALNLVSSIETNAYHYIEILSRAVDSLLPTPEKEPTAEFLTSYITYARNNINPVISEEAAKELVNAYVGMRKLGEVEKIQQVSKRDDIYDILSRSLAPSIYEMDDVKKGLLLQLFGGTNKSFHKGASPRYRGDINILMCGDPSTSKSQILKYVHKIAPRGVYTSGKGSSAVGLTAYITRDQDTKQLVLESGALVLSDGGICCIDEFDKMSDATRSILHEVMEQQTVTVAKAGIITTLNARTSILASANPIGSKYNPDLPVTKNIDLPPTLLSRFDLVYLILDRVDETLDRKLANHIVSM",
    "response#3": "MYEYLSRSIAPEIYGHLDIKKALLLLLIGGVTKEMGDGMHIRGDINICLMGDPGVAKSQLLKYIAKVAPRGVYTTGRGSSGVGLTAAVMRDPVTDEMVLEGGALVLADNGICCIDEFDKMDDSDRTAIHEVMEQQTISISKAGITTTLNARTSILAAANPLYGRYNPRVSPVENINLPAALLSRFDVMFLILDTPSRESDEELANHFFTKYISYARATRHPRISEDAMSVLLDFYVHVRREAHQQTLDALSNSGGTKAQTPIIQVTARQLESLVRITESMARMRLDVLAHRADAEEAIRLFKSFIRKYVQYAKERIVPVLTQDAVNIIVKTYCDLRNDQNTKKSPITARTLETLIRLSNAHAKVRLSKLVEARDANVAAQLLEEVMEQQTISISKAFSLRTLSSSLLNRMVSIEGIVTRCSLIRPKVVKSVHYNEVKDKFHFREYRDQTMTNGVTTSSVYPHEDEEGNPLMTEYGFCTYRDHQTISIQEMPERAPAGQLPRGVDVILDDDLVDKVKPGDRIQLVGIFRTP"
  },
  {
    "reference": "MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFTQFVPGHVHLRDLGKLVAEQIEASGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMAALRLNIPAIFVSGGPMEAGKTKLSDQIIKLDLIDAMIQGANPKVSDEDSNQIERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKALFLNAGKRIVELTKRYYEQNDDSALPRSIATKAAFENAMMLDIAMGGSTNTVLHLLAAAQEGEVDFTMADIDRLSRQIPHLCKVAPSTQKYHMEDVHRAGGVIGILGELDRAGLLNRQVKNVLGLDLLQTLNQYDVMQTVDESVKEMYSAGPAGIRTTQAFSQDCRWPSLDTDRVEGCIRDIEHAYSRDGGLAVLFGNLAIDGCIVKTAGVDEGSLTFRGPAKVYESQEDAVDAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTTYLKSVGLGKSCALITDGRFSGGTSGLSIGHVSPEAANGGLIGLVQDGDIIDIDIPLRKIRLDVDERILATRKEVEFARGDKSWTPKARERQVSLALRAYASLATSADKGAVRDKSKLGG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. We require a protein that can specifically bind to the metabolites produced in the L-valine from pyruvate: step 3/4, L-valine biosynthesis pathway. The designed protein sequence is ",
    "response#1": "DVVHRASDALMAEGGIVILRGSLAPEGAVMKTAGGNLRRVDGVARVFDGEQAAFDAVTGGRIEKGSVIVIRNEGPAGGPGMREMLAVTAAVKGAGLGGDVALLTDGRFSGATHGYMVAHVAPESVRGGPIGVVRDGDRVIIDPTERRLELDVPEAELAERRKSWQEPPARFERGAMAKYAKLVGSASRGR",
    "response#2": "TFGDEAETDLFGEQAVLCGGMMGLAQAAYETLVEAGYPPLLAYMECVHEIKQVADLLYARGPAGMRSAISNTAEFGAYSATPRMVDDATKTAMRTLLKEIKDGTFTRKMQADHDAGAPWFRAQRAIAKAHPMEQSSADVRALMPWR",
    "response#3": "DVVHRASDALMAEGGIVILRGSLAPEGAVMKTAGGNLRRVDGVARVFDGEQAAFDAVTGGRIEKGSVIVIRNEGPAGGPGMREMLAVTAAVKGAGLGGDVALLTDGRFSGATHGYMVAHVAPESVRGGPIGVVRDGDRVIIDPTERRLELDVPEAELAERRKSWQEPPARFERGAMAKYAKLVGSASRGY"
  },
  {
    "reference": "MGGKWSKSSIVGWPAIRERIRRTPPTTPPPAATGVGVVSQDLDKHGAVTSNNMTNESCTWLEAQEDEDVGFPVRPQVPLRPMTYKGAFDLGFFLKEKGGLDGLIHSKKRQEILDLWVYHTQGYFPDWQNYTPGPGIRYPLCFGWCFKLVPVDPREVEEANEGENNCLLHPMSQYGMDDEEKEVLMWKFDSSLARRHVAREMHPEYYKDC",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis; Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Decreases the half-life of TP53, protecting the infected cell against p53-mediated apoptosis; Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike.2. The designed protein should be involved in suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II, suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I by localizing to virion component, host cell Golgi membrane, membrane, extracellular region and exhibiting GTP binding, SH3 domain binding.3. The protein design must feature the PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization, Diacidic; necessary for CD4 internalization, Dileucine internalization motif; necessary for CD4 internalization motif. The designed protein sequence is ",
    "response#1": "MGGKWSKSSIVGWPQVRERMRQTPPAAEGVGAVSRDLERHGAITSSNVNHPSCVWLEAQEDEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLVWSQKRQDILDLWVYHTQGYFPDWQNYTPGPGVRYPLTFGWCFKLVPVEPEEVEKANEGENNCLLHPMSQHGMDDPEKEVLMWKFDSRLAFHHVARELHPEYYKDC",
    "response#2": "MGGKWSKRSSEGWPAVRERMERAEPAAEGVGAVSRDLEKHGAITSSNTAATNADCAWLEAQGEEEQVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYNTQGYFPDWQNYTPGPGTRFPLTFGWCFKLVPVEPEKVEEANEGEDNCLLHPMSQHGMDDPEKEVLMWKFDSRLAFHHVARELHPEYFKDGG",
    "response#3": "MGGKWSKRNLPGWPAVRERMRRTEPAAEGVGAASRDLEKHGALTTSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSQKRQEILDLWVYHTQGYFPDWQNYTPGPGTRYPLTFGWCFKLVPVDPAEVEEANEGENNCLLHPMSQHGMDDPEREVLMWKFDSRLAFHHMAREKHPEFYKDC"
  },
  {
    "reference": "MSPARPCRGLRAVVAASMGLSEGPAGSARSGRLLRSPSPTPGARLLRLPGSGAGRAANPERGGWTEALRAAVAELRAGAVVAVPTDTLYGLACSASCSEALGAVYRVKGRSETKPLAVCLGRVADVYRYCHVRVPEGLLKDLLPGPVTLVMERSEELNKDLNPFTPLVGIRIPDHAFMQDLAQMFGGPLALTSANLSSQSSSLNVEEFQDLWPHLSLIIDGGPIGDGQSPECRLGSTVVDLSVPGKFGIIRPGCALESTSAILQEYGLLPSHGSCW",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Cytoplasmic and mitochondrial threonylcarbamoyl-AMP synthase required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine; May regulate the activity of some transporters .; Catalyzes the conversion of L-threonine, HCO(3)(-)/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate2. The protein contains novel YrdC-like domains that confer a unique function or activity.3. The designed protein should be involved in tRNA threonylcarbamoyladenosine modification, regulation of translational fidelity by localizing to plasma membrane, cytoplasm, mitochondrion and exhibiting tRNA binding, L-threonylcarbamoyladenylate synthase, double-stranded RNA binding, nucleotidyltransferase activity. The designed protein sequence is ",
    "response#1": "MRVLGIETSCDETGIAVYDDELGLLSHTLYSQVKLHADYGGVVPELASRDHVRKIVPLIRQALKDANTEMADLDGIAYTKGPGLIGALLVGACVGRSLAFAWDKPAIGVHHMEGHLLAPMLEDDAPEFPFVALLVSGGHSMLVKVDGIGRYEVLGESVDDAAGEAFDKTAKLMGLDYPGGPRLAKLAAKGLPAGYKFPRPMTDRPGLDFSFSGLKTFTANTIAAEPDDEQTRANIARAFEEAVVDTLAIKCRRALKQTGYNRLVIAGGVSANTRLRETLAEMMNSLGGQVFYPRGEFCTDNGAMIAFAGLQRLKAGQHEDLAVKGQPRWPLDLQRRRRRDG",
    "response#2": "MRVLGIEGTAWCASAALYDAETDTVLIESDPYEPDSGGIHPREAAEHMSEAIPAVVDRVLTAAEERHGPDAVDAVAFSRGPGLGPCLRIVGTAARSLAGTLGVPLVGVNHMVAHLEIGRHRSGFDNPVCLNASGANAHLLGYHDGRYRVLGETMDAGVGNAIDKFTRHVGWSHPGGPKVEAAAAEFATAASGAGSEGEKAGSEEEGPESTPGADLLDLPYVVKGMDFSFSGISSAANDAADEGVPVEEICFSLQEHVFAMLTEVSERALSLTGADELVLGGGVAQNDRLREMLAAMCEARGAAFHAPEPRFLRDNAGMIAVLGAKMAAAGDTVAVAESAVDPNFRPDQVPVTWRDPAGADAGTTALPAGTLVTGRRVRGRTEARTRLDGGAVAPGTDAADTGTPGDAPGPLTVVARRPDGEPATRYRAVKLGRPPAGGPDGPGADGPGADGPGADGPGADGPGADGPGADGPGADGPGADGPGADGPGADGPGADGPGADGPGADGPGADGPGADGPEADGPGADGPGADGPGVDGPGTDGLGADGPGVDGPGADGPGTDGGRPGSERVPRPQQVAGPTPPRPATLPPRPPRPQGTGGPDGQAPAGGDARPRPAQLPPRPPRPAAAAVAGAPAAPGPTALPARPARPTIPGRAGLPAVPGRPSGPTAPTAPTAPTAPTAPTAPTAAAGPGGPAGSGGAGPAGSVGLG",
    "response#3": "MRVLGIEGTAWCASAALYDAETDTVLIESDPYEPDSGGIHPREAAEHMSEAIPAVVDRVLTAAEERHGPDAVDAVAFSRGPGLGPCLRIVGTAARSLAGTLGVPLVGVNHMVAHLEIGRHRSGFDNPVCLNASGANAHLLGYHDGRYRVLGETMDAGVGNAIDKFTRHVGWSHPGGPKVEAAAAEFATAASGAGSEGEKAGSEEEGPESTPGADLLDLPYVVKGMDFSFSGISSAANDAADEGVPVEEICFSLQEHVFAMLTEVSERALSLTGADELVLGGGVAQNDRLREMLAAMCEARGAAFHAPEPRFLRDNAGMIAVLGAKMAAAGDTVAVAESAVDPNFRPDQVPVTWCEGGVIAYPTEAVWGLGCDPWNEVAVDRLLALKERPVHKGLILVADDIEQFDFLLDDLPAIWQERLAASWPGPNTWLVPHQDRLPAWITGVHDSVALRVSDHPLVRALCRLTGPLISTSANPAGRPAARSRLRVEQYFPRQLDKVLGGALGGRKNPSLIRDLRTGDVIW"
  },
  {
    "reference": "MLAKTGDVVVQKVPVIRLSVFLHFFFVFPFCLLHRLYMGMKQVQEFIMEPKGSVFVVRATLRVSLENAGKIFFNETE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "MMSLWDVIVSP",
    "response#2": "GHN",
    "response#3": "ETEG"
  },
  {
    "reference": "MDDDGLLLNFAAPDSNASSKSSAQKPVKVSGGRWKDRRKLQLSLSGRGRNKRAETGVNKESIAQPRDFSSSKPQSFREEQLAKRPKYFESRGEGGKNETYVSSLFTSNTKSKLENEKVEEEKTYLPSNAPMKGADDFNGLGLNDNLVHHLTESLRFKNPTQIQKSVIPSLLSTSRDLFVKAQTGSGKTLSFLLPILHKLMQEKKNPITRESGVFAIVLVPTRELANQIYGVLETLTRCHHQIVPGIVIGGEKKKSEKARIRKGVNILVATPGRLADHIENTTSLDLSQLRYLILDEGDRLIDLGFEETITKITDTITRCSRISESTQKWQGLPTKRVNVLCSATMENNVEKLGSIILNNPEQISIDTSKSREGDEIDSKSMAPAQLTQRVVVVPAKLRLVTLSAVLKEVAKTAPTSSTEIVRTIVFFSCSDSVNFHYEAFKRNGSEFRKARNAETNRFEMVTVGEDEANAEGSDTEIPKISSAPTISANSVVYKLHGSLTQQVRTSTLQSFVQAVPFDNSENNYNHLILLCTDVASRGLDLPNISSVVEYDPPFSVQDHLHRIGRTARLGNKGSSYLFLLPGIEEGYVDGKIRVVHPEGSIRITNYETILQNAFGDSSEIKKSDPKSKQGKWDMHATTWHLDIERWLLEDAASHESAKQAFTSHIRAYATHLSSEKTFFNVKTLHLGHLAKSFGLREPPKKLGSLATKSSSTRKEYGEKSRKLEDPRKKMLRMAKLAASSASSEFNY",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein have enhanced ATP + H2O = ADP + H(+) + phosphate.2. The protein design must be able to perform ATP hydrolysis activity, ATP binding, RNA helicase activity in order to contribute to rRNA processing.3. The designed protein should contain one or more Helicase ATP-binding domains that are essential for its function.4. Incorporating a DEAD box motif is critical for the successful design of the protein. The designed protein sequence is ",
    "response#1": "EMDKAVNMKESPTKHSTIVFAATKHHVDYLYSLLREAGFAVSYAYGSLDQTARKIQVNNFRTGLSNILVVTDVAARGIDIPILANVINYDFPSQPKIFVHRVGRTARAGRKGWSYSLVRDADAPYLLDLQLFLGRRLVLGREHGDQVDYAEDVVR",
    "response#2": "GYKSPTPIQAESIPLALQGRDLIGLAETGSGKTAAFALPILQALMNKPQSLFGLILAPTRELACQISEAFEALGSLISVRCAVIVGGMDMVSQAISLGKKPHIIVATPGRLLDHLENTKGFSLRSLKYLVMDEADRLLDLDFGPILDKILKVLPRERRTYLFSATMSSKVESLQRASLSNPLRVSISSNKYQTVATLLQL",
    "response#3": "GLRQLLHPTARTATDVHLRPPFLIFTQTIPRAVALHSELLYDIPPEAGGSSRIAVLHSELSDSQRSEIMKGFRKGEIWILVTTDLLARGVDFRGINGVVNYDIPNSAAVYVHRVGRTGRAGREGGVAVTYYTKEDIPYVKSIANIIDVSEKLRGAGGEKSVQKWLLDSS"
  },
  {
    "reference": "MLKGVSLYLVGMMGSGKSTVGRLLAEKLGYGFVDLDALIEQVSGKRVGEIFEREGEAVFRDLESRVLAEVSAYTRLVVATGGGVVLARRNWGYLHHGVVVWLDADIETLLGRVEHEPGTRPLLAGGDRHLRLVELLGERARLYAQADVRVSAAGLPPAVAEETLRCLAARLAEDSAP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should take into account the physiological conditions of the Metabolic intermediate biosynthesis pathway.2. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.3. A protein with ATP binding functions is required to enable phosphorylation, chorismate biosynthetic process, amino acid biosynthetic process.4. The designed protein should have a binding site that is specific to the target ATP ligand. The designed protein sequence is ",
    "response#1": "ALPILVRHFRQKELDDAVVVAVDIGISKRARDFAEQLDLPLAIVEKRRIGNMEKSESLNIIGDVEGRTCITFDDEIDTAGSLISAATALRESGAKEIFACCTHGVLSKEAAKRLIDSDINEVVMTDH",
    "response#2": "ALPILVRHFRQKELDDAVVVAVDIGISKRARDFAEQLDLPLAIVEKRRIGNMEKSESLNIIGDVEGRTCITFDDEIDTAGSLISAATALRESGAKEIFACCTHGVLSKEAAKRLIDSDINEVVMTDAR",
    "response#3": "AAPVFIDYIRELNLKNLTIVSPDTGGIKSARAVAQRLNASLAAIDKRRMTGKDIQVMNIMGEVDGCDLVIVDDIVSTAGSLTEAIKALRANGANSIYAAITHPILAGPAIERISGFG"
  },
  {
    "reference": "MENRWQVMIVWQVDRMRISTWKSLVKHHMYVSKKAQGWFYRHHYDCTHPRVSSEVHIPLGDARLVIKTYWGLHTGERDWHLGQGVSIEWRKGRYSTQVDPGLADQLIHMHYFDCFSDSAIRKAILGQVVRPRCEYSAGHNQVGSLQYLALKALVTPTRERPPLPSVQKLTEDRWNKPQKTKDHRGSHTMNGC",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that May interact directly with APOBEC3G mRNA in order to inhibit its translation; Seems to play a role in viral morphology by affecting the stability of the viral nucleoprotein core; Finally, Vif also contributes to the G2 cell cycle arrest observed in HIV infected cells.; In turn, they are directed to the 26S proteasome for degradation; Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism2. The protein should be optimized for stability and expression in host cell plasma membrane where it will participate in viral life cycle. The designed protein sequence is ",
    "response#1": "MEPVDPSLEPWNHPGSQPKTACNNCYCKKCCFHCQVCFLKKGLGISYGRKKRRQRRRTPQDSQIHQVSLPKQPTSQPRGDPTGPKESKKKVESKTETDQFD",
    "response#2": "MEPVDPSLEPWNHPGSQPTTACSKCYCKRCCYHCQKCFLTKGLGISYGRKKRKHRRGTPQSRQDHQNPIPKQPIPQTRGNPTGPKESKKKVESKTETDRFD",
    "response#3": "MKVKGTRKNYQHLWRWGTILLWIIIICSAGKRQ"
  },
  {
    "reference": "MARRYLLEFEKPLVELEKQIEQIRELARDSEVDVSQQLLQLETLAARRREEIFNALTPAQKIQVARHPQRPSTLDFIQMFCDDWVELHGDRNCSDDKALIGGIARIEEKSVLVIGQQKGRDTKENVARNFGMAKPGGYRKALRLMNHADRFKLPIISFIDTPGAYAGLLAEEQGQGEAIAVNLREMFRLRVPIISTVIGEGGSGGALGIGVADRLLMFEHSVYTVASPEACASILWRDAGKAPDAAAALKITGSDLMALGIVDEVLSEPSGGNNWAPLKAGEVLKESLIRNLRELDSLSIRQLRDKRYEKFRQMGRFLEPSSLDEELIT",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that First, biotin carboxylase catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the carboxyltransferase to acetyl-CoA to form malonyl-CoA.; Component of the acetyl coenzyme A carboxylase (ACC) complex2. The protein should be optimized for activity in malonyl-CoA biosynthesis, malonyl-CoA from acetyl-CoA: step 1/1, Lipid metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. We need a protein that can perform acetyl-CoA + N(6)-carboxybiotinyl-L-lysyl-[protein] = malonyl-   CoA + N(6)-biotinyl-L-lysyl-[protein] with high efficiency.4. The designed protein should contain one or more CoA carboxyltransferase C-terminal domains that are essential for its function.5. The designed protein should have acetyl-CoA carboxylase activity, carboxyl- or carbamoyltransferase activity within acetyl-CoA carboxylase complex to regulate fatty acid biosynthetic process, malonyl-CoA biosynthetic process. The designed protein sequence is ",
    "response#1": "MQKSGKSEACRNATGKLGGSDAVISGMDFGFIGGSMGSVVGEKIARAADKSVELNAPLIVISQSGGARMMEGAFSLMQMAKTSARLTRLGERGIPFISLMTDPTMGGISASFAMLGDLNISEPKALIGFAGPRVIRDGLWIKCPDSGHLVFYKDVEANQFVIPGSNYHMRMGAVARLKSVFDGETWYDIALPEVTADPLKFRDERRYVDRIKDARAKTGLHDAVKVGYGKLENFPVVIAVQDFDFMGGSLGMAAGEAIVRGMELALEKRAPFILFAASGGARMQEGILSLMQMPRTTVGVQMLREAKLPYIVVLTNPTTGGVTASYAMLGDVQLAEPGALIGFAGARVIEQTIREKLPEGFQRAEYLRDHGMVDIVVHRHDLRSTLARLCRILTKSPAAEIEVVTPEKARKELQRIYSKLGAWQTVQVARHPQRPYTMDYVQALFTEVQVLAGDRAFADDQAIIGGLARFNGQPIVWMGHQKGRDTKEKIQRNFGMPRPEGYRKALRLLRLAERFALPVFTFIDTPGAYPGIGAEERGQSEAIARNLAVMSDLAVPIICTVIGEGGSGGALAIGVGDRMLMLEYGVYSVISPEGCASILWKNAAMAAEAAETLGITARRLQGMGLVDEVLAEPLGGAHRDPEAVFALARERFAHHLQQASAAVLAQKKDARAKTGLHDAVKVGYGKLENFPVVIAVQDFDFMGGSLGMAAGEAIVRGMELALEKRAPFILFAASGGARMQEGILSLMQMPRTTVGVQMLREAKLPYIVVLTNPTTGGVTASYAMLGDVQLAEPGALIGFAGARVIEQTIREKQ",
    "response#2": "MQKSGKSEACRNATGKLGGSDAVISGMDFGFIGGSMGSVVGEKIARAADKSVELNAPLIVISQSGGARMMEGAFSLMQMAKTSARLTRLGERGIPFISLMTDPTMGGISASFAMLGDLNISEPKALIGFAGPRVIRDAKTELEDTIVAGLGLVQGVKLVAVVHEFNFIGGSLGMAAGEAIIRAFEKAIAEKCPLVMFPASGGARMQEGILSLMQLPRTTVAVNLLKEAGLPYIVVLTNPTTGGVTASYAMLGDIHLAEPGAEIGFAGKRVIEQTLRERELMKEREAKAKPFKVWDKVSAARQIKRLASVDYMDYIFDEFMELHGDRYFRDDPAIVGGIAYLDGQPVTVIGVHKGKDLEDCAKRNYGMPSPEGYRKALRLMKQAEKFNRPIITFVNTSGAYPGMEAEENGQGEAIARNLYEMSGIKVPILCLMIGEGGSGGALALSVGNEVWMMENATYSILSPEGFASILWKDGKRAKEAAEVMKITAHDLKELDVVDDIIPEFGGADEDALSSIGNYMKGNMKKFLETLEERLARLEEDIYGNLKPWNRVQMARHPDRPTTMDYIERIFTDFIEFHGDRFYGEDEAIISGIGYYKDSPVTVIGHQRGKDTKENIRRNFGMPHPEGYRKALRHMQQAEKFNRPIITFIDTKGAYPGKAAEERGQSEAIAKNLMEMAGLQVPIICIVIGEGGSGGALGLGVGNHIHMLENSTYSVISPEGAAAILWKDSTQAKKAAETMKITAPDLKELGIIDQVISEPRGGAHRDVDSQAELIDNIIKQSLVELEEEVSKLKEKSDRLKEKIFSELGAWQVSQLARHPLRPYTRDYIERIFTEFDEFAGDRTFANDPAILGGIARLDGEPVMVIGQQKGRGTAEKIKRNFGMPKPEGYRKALRLMEMAERFKMPIMTFIDTPGAYPGVGAEERGQSEAIARNLKVMASLKVPTICTVIGEGGSGGALAIGVGDRVNMLQYSTYSVISPEGCASILWKSADKAPLAAEAMGVTAKRVKELDLINNLVDEPLGGAHRNYDAMARNLKVRLKRDLA",
    "response#3": "MQKSGKSEACRNATGKLGGSDAVISGMDFGFIGGSMGSVVGEKIARAADKSVELNAPLIVISQSGGARMMEGAFSLMQMAKTSARLTRLGERGIPFISLMTDPTMGGISASFAMLGDLNISEPKALIGFAGPRVIRDPHQHAELMKEREAKAKPFKVWDKVSAARQIKRLASVDYMDYIFDEFMELHGDRYFRDDPAIVGGIAYLDGQPVTVIGVHKGKDLEDCAKRNYGMPSPEGYRKALRLMKQAEKFNRPIITFVNTSGAYPGMEAEENGQGEAIARNLYEMSGIKVPILCLMIGEGGSGGALALSVGNEVWMMENATYSILSPEGFASILWKDGKRAKEAAEVMKITAHDLKELDVVDDIIPEFGGADEDALSSIGNYMKGNMKKFLEEINKKLEQTKKDIYKNLTAWQRVQLSRHPNRPYTLDYIKAICGDTFLELHGDRGFKDDKAMVGGLGKINGQSFMIVGQQKGYNTKTRQYRNFGMANPEGYRKALRLMKMAEKFGIPVLTLVDTPGAYPGLEAEERGQGEAIARNIFEMVRLQVPIITIIVGEGASGGALGIGVGDRVYMLENTWYSVISPESCSSILWKSWEYKERAAEALKLTSSDMKKQKLVDDVIPEPLGGAHYDRETTFKTVADYITKGYNRLWELELKGL"
  },
  {
    "reference": "MHSKLNNIEEAISDIKKGKVIIVVDDENRENEGDFIAAAETITPEIVNFMATYGRGLICAPLTEDRCDELALELMVTKNTSSHETPFTVSIDLLGYGCTTGISVSDRAKTINALTNPNIKPEELGRPGHIFPLKAKRGGVLQRTGHTEAVTDLTRLAGLKPAGALVEIMNEDGTMARLPELFKIAKKFDLKIISIEDLVAYRMRYESLITKEVSVSLPTLYGDFQLIAYKQKTNNQEHLALVKGSWEKDEPVLVRVHSSCVTGDIFGSLKCDCGDQLHTAMEMIEKEGKGIVLYMNQEGRGIGLMNKLKAYHLQELGKDTVDANIELGFKADERDYGIGAQILRDLGATKLRLITNNPTKRAGLIGYGLQIVENIPLIIKPNEHNLRYMKTKRDKMGHQLEIS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein contains novel GTP cyclohydrolase II domains that confer a unique function or activity.2. We require a protein that can specifically bind to the metabolites produced in the riboflavin biosynthesis pathway.3. The protein must have high specificity towards manganese ion binding, magnesium ion binding, GTP binding.4. The GTP, D-ribulose 5-phosphate, Mg(2+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "RVATAHLPTQWGHFIAHAYEADIEANPYIALVMGDVTSEEPVLVRIHSGCLTGDALRSLRCDCGDQLDLAMRRIAEEGRGVLLYIQQEGRGIGLVNKLKAYVLQDHGADTVEANELLGFPADIRDYSLGAQVLEDLGLRRIRLMTNNPAKFAALEGYDLEIVERVR",
    "response#2": "ATATLPLKNFGDFTMTVFNSLLDGSEHFALMKPPAVINRVPLVRIHSECVTGDVLGSCKCDCGAQLEQSLAMLAVEGGVLIYLRQEGRGLGLANKLKAYALQEQGFDTVDANIKLGLPIDNRDYAVAYQILNHFGISILRLLTNNXXXVANLEKYGLKISERI",
    "response#3": "STRIPLQNKGDFNMTVFANELDCAEHFALVKPPAFANRVPLVRIHSECITGDVFGSCKCDCGKQLEQSLSLIAAEGGILIYLRQEGRGIGLANKLKAYALQEQGLDTVDANHQLGLPVDGRDYAIAYQILKYFGIDAIRLLTNNPSKIAAVDRYGIKVMERVK"
  },
  {
    "reference": "MELNNLKPAAGAKHAKRRVGRGIGSGLGKTAGRGHKGQKSRSGGFHKVGFEGGQMPLQRRLPKRGFTSLTKEFVGEVRLGDLEKLPVDEIDLLALKQAGLVGELTKSAKIIATGELKRKIVVKGLGATKGARAAIEAAGGSFAE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Binds to the 23S rRNA..2. The protein can be targeted to cytoplasm, large ribosomal subunit and perform structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "GFKEGEIVNVLGVSKGKGFAGVVKL",
    "response#2": "MNVIGTITAPHGVRGTVRVRPAGEGRHLREGLSPLVGGRRRRILRARRTPKGFLVDLEGVPDRFRAAELRGEDLLLDRR",
    "response#3": "MPN"
  },
  {
    "reference": "MTTLTVYSDRDGATPLATYEDFATIRAQLSALDIELERWQASHPLRANATPEEVLEAYREPIETLKQKYGFVSVDVVSMYPDHPSAAEARGKFLAEHTHDDFETRFFVDGAGAFYVHADGKVFMLVCTRGDLINLPANTTHWFDMGAKPTFKAIRFFRIAEGWVGRFTGSDIAAQFPKYEPAA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4- methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway2. The protein design should consider the endogenous proteins and enzymes present in the L-methionine biosynthesis via salvage pathway, Amino-acid biosynthesis pathway to avoid interference with native metabolic processes.3. The protein should have a high affinity for Fe(2+), Ni(2+).4. The designed protein must be optimized for iron ion binding, nickel cation binding.5. The protein should have a fast turnover rate for 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-   methylsulfanyl-2-oxobutanoate + formate + 2 H(+). The designed protein sequence is ",
    "response#1": "MLTKDDLQQLQALAHVQASDAELDTFAKDLDQIIQFYEQIDTLDTTGVAPMSQVVPIENVSRKDVPEPYLDQVPKEE",
    "response#2": "MRRLGFMQPYSFHDPKTLAREELDARYLVADSEIMAACRASGATHIIIPPAGSFDGSLAPLVERFAQDSGVDLVFAGYPNMTDTGLYPQNYHQHPERVLRNCNVMIGGTSVLHRRITELEAPTISACWDASRTTHYWGYDQAAFWMLRRGLPVTVSLQPTHVANVLMHEHSMLYEMRCTIEGQRAEGIPVEEVMAKAPDLYAKYAEGEAGLRQVAYAAVDGISHFMGRAPDYADPAMEERLVALRERFPV",
    "response#3": "MAAPETAAPEAAAAEPVEAAPAVAAPAVTAPAVTAPAVTAPAVTAPAVTAPAVTAPAVTAPAVTAPAPTAPAPTAPAATAPAASAPGASAPAASAPAAPAAPAPAAPAAAAAPAAAAAPAVAAAPVAAEPAEAAPAQAAPAQAAPAQAAPAQAEPAPAQAAQAEPEPVAAEAAPPQAEPEPAAAEPAPAQAEPEPAAAEPAPAQAEPEPAAAEPAPAQAEPEPAAAEPAPAQAEPEPAAAEAAPAQAEPEPAAAEPAPAQAEPEPAAAEPAPAQAEPEPAAAEAAPAQAEPEPAAAEPAPAQAEPEPAAAEPAPAQAEPEPAAAEPAPAQAEPEPAAAEPAPAQAEPEPAAAEPAPAQAEPEPAAAEPAPAQAEPEPAAAEPAPAQAEPEPAAAEPAPAQAEPEPAAAEPAPAQAEPEPVAAEAAPPQAEPEPAAAEPAQAAPAQAAPAQAAPEPAAAESAPAAAEPAQAEPEPAAAEPAPAEAEPAQAEPEPAAAEPAPAQAAPEPAAAEPAQAEPEPAAAEPAPAEAAPEPAAAEPAQAAPAQAAPEPAAAEPAQAEPEPAAAEPAPAEAAPEPAAAEPAQAEPEPAAAEPAPAEAAAEPAQAEPEPAAAEPAPAEAAPEPAAAEPAQAEPEPAAAEPAPAEAAAEPAAAEPAQAEPEPAAAEPAQAEPEPAAAEPAQAEPEPAAAEPAQAE"
  },
  {
    "reference": "MSQDPYNKSDNKLPRFNNKPSGGENPKKGPKFSIYWVYAIIFAVLIGFNFFGPLSTNIAKTNPNAFKQMLIEGDVEKFIIVSNKNMVRVYLEKDKIAKYNDNVERGVTGKINQEGPHLYFKITGEDFQREIREFYEANPNIEEVLYEVEQERDWFSGVLNLLLPVLLFIALWILLMRKMGGGGGSGGGPGGIFNIGKSKATLFDKGTKVNITFKDVAGLDEAKVEVMEIVDFLKNPKKYTNLGGKIPKGALLVGPPGTGKTLLAKAVAGEAQVPFFSLSGSDFVEMFVGVGASRVRDLFKQAREKAPCIIFIDEIDAIGRARGKNAMMSNDERESTLNQLLVEMDGFGTDVGIIVLAATNRPDVLDSALLRPGRFDRQISIDKPDLKGREAIFKVHLKPIKISTKLDIHKLAEQTPGFAGADIANVCNEAALIAARKGKEHVEMEDFQDAVDRVIGGLEKKNKIISPEEKKIIAYHEAGHAICGWYLEHAYPLLKVTIVPRGVAALGYAQYTPKEQYLYNTDQLTDQICMTLGGRASEDIFFGKISTGAQNDLQQITKIAYAMISVYGMNEKVGNVSFYDPQQGEYNFTKPYSEETSRLIDDEVRILINEAYRRTKDLLEEKRDKVEILAEALLIREVLFQSDVEALIGKRPYEEKKILEDDDEDDDDKKIEKDKHDTGVISEGVPPYDSNVTNHPPGL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein can be targeted to plasma membrane and perform zinc ion binding, ATP binding, ATP-dependent peptidase activity.2. The protein design should emphasize Polar residues compositional bias.3. The protein should have a high affinity for Zn(2+).4. The designed protein have AAA+ ATPase domains with specific sequence motifs that are crucial for its activity.5. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "IPRGVLLVGPPGTGKTLLAKAIAGEAGVPFFSMSGSEFVEMFVGVGASRVRDLFRKAKESAPCIVFIDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNSGVIIIAATNRPDVLDSALMRPGRFDRQITVDLPGLEERERLIHRHFADDL",
    "response#2": "IPKGVLLVGPPGTGKTLLARAIAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQGKKAAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLNQMLVEMDGFESNEGVILIAATNRPDVLDPALLRPGRFDRQVVVPNPE",
    "response#3": "APKGVLLVGAPGTGKTLLARAVAGEAGVPFFSLSAAEFVEMIVGVGAARVRDLFKQAREQAPAIIFIDEIDSIGRARGPTAIGGSSEQEQTLNQILTEMDGFSSREGIIVLAATNQPEILDKALLRPGRFDRRVVVNLPS"
  },
  {
    "reference": "MKLRFQRVALVGKYQAPTSAGMSDSSRDALDGIARFLANEGCEVALEADTAANTGFTDYPALSVERIGLDCDLCLVVGGDGTMLGVGRQLAQYRTPLIGINQGRLGFITDIPLGEYPTVLKPMLRGEYEEDLRPLMRARVMRQGQCVFEALAMNDVVVNRGSTSGMVELRVEVGGHFVSNQRADGLIIASPTGSTAYALSAGGPMLHPTIPGWVLAPIAPHTLSNRPIVLSDSMEVAVEVVSGRDVSANFDMQSLASLQHGDRILVQRSDYRARFLHPRGWNYFATLRKKLRWNEGGY",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should have a high affinity for NAD(+).2. For general function, the protein need meet that Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.3. The designed protein should have NAD binding, metal ion binding within cytoplasm to regulate NAD metabolic process, phosphorylation. The designed protein sequence is ",
    "response#1": "MTIFP",
    "response#2": "EGGRR",
    "response#3": "MFSVR"
  },
  {
    "reference": "MTKFVFVTGGVVSSLGKGIASASLAAILESRGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFITTRMRRANNFTTGQIYKSVLEKERRGDYLGKTVQVIPHVTNEIQEFVKRGAGVGTPDAVDVAIVEIGGTVGDIESLPFLEAVRQMSLKLGPNNTAFVHLTYVPWIAAAGELKTKPTQHTVQKLREIGIQPDALLCRADRRIPDDEREKISLFTNVPEYGVISMWDVDTIYKVPRVLHEQGLDQLICTKLHLSTRPADLSKWDRLVHAVEHPQREVTIAMCGKYVDLSDSYKSLNEALRHAGIHNQARVKIEYVDSEELTPQNVERLGKYDAILVPGGFGKRGVEGKILAAQYAREHRIPYLGICLGMQVATIEYARHKAGLAGANSTEFEPQAPHPVIALIDEWQDADGSIQKRDANSDLGGTMRLGSQSSDVKPGTLAHEIYGDVVTERHRHRYEANEKYLPQLEEAGLVISAITQREKLTEIVELPRDVHPWYMGVQFHPEFKSTPWGGHPLFNSYIKAALAHQAERQPA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The presence of Glutamine amidotransferase domains is critical for the interaction of the designed protein with its intended targets.2. We require a protein that can specifically bind to the metabolites produced in the CTP biosynthesis via de novo pathway, Pyrimidine metabolism pathway.3. A protein with glutaminase activity is needed to enhance 'de novo' CTP biosynthetic process, glutamine metabolic process in a specific context.4. The Nucleophile, active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur. The designed protein sequence is ",
    "response#1": "LRDSYKSIVEAIYHAAAELDVKPVLHWVEAEDVTSPKYVSEAFREVHGVIILPGFGLRGAEGKINCITYARTHNVPFLGICFGLQLAVVEYARNVIGLREANSTELDPNTPHPVVTLLPEQGGIKELGGTLRLGAYPIKLIKGSIVYSLYGKEVIYERHRHRYEVNPAYLPLLRKGGLRVTGFSLNSSRVEFIELPSHYFFVATQAHPEFKSRPGSPSPLYYGFIIR",
    "response#2": "LCGKYVKLRDSYKSIVEAIYHAAAELDVKPVLHWVEAEDVTSPKYVSEAFREVHGVIILPGFGLRGAEGKINCITYARTHNVPFLGICFGLQLAVVEYARNVIGLREANSTELDPNTPHPVVTLLPEQGGIKELGGTLRLGAYPIKLIKGSIVYSLYGKEVIYERHRHRYEVNPAYLPLLRKGGLRVTGFSLNSSRVEFIELPSHYFFVATQAHPEFKSRPGSPSPLYYGFL",
    "response#3": "LCGKYVKLRDSYKSIVEAIYHAAAELDVKPVLHWVEAEDVTSPKYVSEAFREVHGVIILPGFGLRGAEGKINCITYARTHNVPFLGICFGLQLAVVEYARNVIGLREANSTELDPNTPHPVVTLLPEQGGIKELGGTLRLGAYPIKLIKGSIVYSLYGKEVIYERHRHRYEVNPAYLPLLRKGGLRVTGFSLNSSRVEFIELPSHYFFVATQAHPEFKSRPGSPSPLYYGFL"
  },
  {
    "reference": "MATMTKKKLISTISQDHKIHPNHVRTVIQNFLDKMTDALVQGDRLEFRDFGVLQVVERKPKVGRNPKNAAVPIHIPARRAVKFTPGKRMKRLIETPTKSS",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.2. The designed protein should have DNA binding, structural constituent of chromatin within cytosol to regulate chromosome condensation. The designed protein sequence is ",
    "response#1": "LPKATVKRIMKQHTDFNISAEAVDELCNMLEEIIKITTEVAEQNARKEGRKTIKARDIKQC",
    "response#2": "PKRSLSAYMFFANENRDIVRAENPGISFGQVGKLLGEKWKALTPEDKIPYENKADTDKKRYEKEKAEYD",
    "response#3": "KSTGGKVPRKQLVIEAAGKSAPSTSGMTKPHRYRPGTVALREIRPYQKSTHFLIRKLPFQRLVREIAQDFKTDLKFQSAAIRTLQEASEAYLVVFLK"
  },
  {
    "reference": "MTKKVVFLDRESLDATVREFNFPHEYKEYESTWTPEEIVERLQGAEIAMINKVPMRADTLKQLPDLKLIAVAATGTDVVDKAAAKAQGITVVNIRNYAFNTVPEHVVGLMFALRRAIVPYANSVRRGDWNKSKQFCYFDYPIYDIAGSTLGIIGYGALGKSIAKRAEALGMKVLAFDVFPQDGLVDLETILTQSDVITLHVPLTPDTKNMIGAEQLKKMKRSAILINTARGGLVDEAALLQALKDGTIGGAGFDVVAQEPPKDGNILCDADLPNLIVTPHVAWASKEAMQILADQLVDNVEAFVAGKPQNVVEA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must be able to NAD binding. The designed protein sequence is ",
    "response#1": "GEVYLIGAGPGAPDLLTFRALRLMQQADVIVYDRLVAPEIIELARRDSEKIYVGKQRQNHALPQESINQLLANMAKAGKRVARLKGGDPFIFGRGGEEIETLMQQGIHFQVVPGITAASGCATYAGIPLTHRDYAQSCTFVTGHLKDGSINLNWTQLAAPNQTIVIYMGLIGLSTICQSLIEHGSSEDLPIALVQQGTTTHQKIVTGTLKTLPAKVVDQNIKAPTLIIVGMVVTLYEG",
    "response#2": "GEVYLIGAGPGAPDLLTFRALRLMQQADVIVYDRLVAPEIIELARRDSEKIYVGKQRQNHALPQESINQLLANMAKAGKRVARLKGGDPFIFGRGGEEIETLMQQGIHFQVVPGITAASGCATYAGIPLTHRDYAQSCTFVTGHLKDGSINLNWTQLAAPNQTIVIYMGLIGLSTICQSLIEHGSSEDLPIALVQQGTTTHQKIVTGTLKTLPAKVVDQNIKAPTLIIVGMVVTLYPD",
    "response#3": "GEVYLIGAGPGAPDLLTFRALRLMQQADVIVYDRLVAPEIIELARRDSEKIYVGKQRQNHALPQESINQLLANMAKAGKRVARLKGGDPFIFGRGGEEIETLMQQGIHFQVVPGITAASGCATYAGIPLTHRDYAQSCTFVTGHLKDGSINLNWTQLAAPNQTIVIYMGLIGLSTICQSLIEHGSSEDLPIALVQQGTTTHQKIVTGTLKTLPAKVVDQNIKAPTLIIVGMVVTLTG"
  },
  {
    "reference": "MSLKRCRALPVVAIVALVASGVIMFIWSQQRRLIYFPSAGPVPSASSVLPAGRDVVVETQDGMRLGGWYFPHTSGGSGPAVLVCNGNAGDRSMRAELAVALHGLGLSVLLFDYRGYGGNPGRPSEQGLAADARAAQEWLSGQSDVDPARIAYFGESLGAAVAVGLAVQRPPAALVLRSPFTSLAEVGAVHYPWLPLRRLLLDHYPSIERIASVHAPVLVIAGGSDDIVPATLSERLVAAAAEPKRYVVVPGVGHNDPELLDGRVMLDAIRRFLTETAVLGQ",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The protein should be optimized for stability and solubility when expressed in plasma membrane, peptidoglycan-based cell wall where it will be involved in suppression by symbiont of host immune response. The designed protein sequence is ",
    "response#1": "LAGWIFFILLSVCTESFSGMVVSQTVAFHFQPHPDLSQFLVMDFTELTVPEAFIQKIGHAFSFFVLTYLLWKQRGSIRSAAAGSFAFAFFTEVLQLFFSRNGCIRDVLIDAVGIGLFYGLYVLFS",
    "response#2": "LQRDWEPWENISDELKVAVIAGEDQKFANHWGFDLPAIQAALAHNERGGNIRGASTLTQQVAKNLFLWSGRSWFRKGLEAWFTALIELFWSKERILEVYLNSAEWGKGVFGAQAAARYHFGVDASRLSRQQAAQLAAVLPSPIKWSASRPSAYVASRAGWIRRQL",
    "response#3": "LAGWIFFILLSVCTESFSGMVVSQTVAFHFQPHPDLSQFLVMDFTELTVPEAFIQKIGHAFSFFVLTYLLWKQRGSIRSAAAGSFAFAFFTEVLQLFFSRNGCIRDVLIDAVGIGLFYGLYVLQ"
  },
  {
    "reference": "MQVKKATVFLNPAACKGKARNLFEKNAAPILHLSGLDITIVKTDYEGQAKKLLELMENTDLIIIAGGDGTVQEVITGLLRRADEATFSKIPIGFIPLGKTSTLSQTLYPGSLNQVQRIADASLAILKGETVPLDVLQIKGEKEQPVFAVTGLRWGSYRDTGVKVSKYWYLGPLKTKAAHFFNTLKEWPQKHQASLTYLGPTKRPPEEPEEKPSRPALYRRIYRRLSLYWASPQKEIPQEATLEAWEEMQLSTIELSITTQNRQLDLTRTEDFMNICIEPDTGTEGSFGIDSEEYEAMPVEVKLLPRKLQFFCDPRRREQMLQPAVQ",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have kinase activity within mitochondrial intermembrane space, mitochondrial inner membrane to regulate glycerolipid metabolic process.2. We require a protein that can specifically bind to the metabolites produced in the Lipid metabolism, glycerolipid metabolism pathway.3. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.4. The designed protein must have at least one DAGKc domain.5. We are looking for a protein with broad substrate specificity for an N-acylsphing-4-enine + ATP = ADP + an N-acylsphing-4-enine   1-phosphate + H(+). The designed protein sequence is ",
    "response#1": "MIGLTAGAYSEAEKKKKKYWYFGPLKFKWAYIRSTLTQWPSVISGKISYIPATEENTKLTKIVIKQPVPEVRRSWWNILYLFYKPRPQHSQEVYEEVEAEDGQDEETISEDVSSMELSVIAPIAQGQQEKIVAPELHIGPSQINRQDFISEGFRREKLPTRVFNSPDDRHSFVKKFKFVPDLPEDAEKKVWYSIDGEQYEAVPVEIKLLRNRLNIFQSV",
    "response#2": "LTDMAWGSYRDTFVKESKYWYFGPIKTFMTHFFSLFKTDIRTPRLFQLAYNPPKPIPKDIEKKTEEAKLMAVNSLISKLLIIEVEEPLAEEKYDAPIEEYGSIEFMASVNNHDPHDKREKAGARIVIESSDFNWLNFLREGPKRIVAGKPIPVKPETVIDCSEFQLVPLSSNESWFGMDNEKFEAFSCHATVIPQ",
    "response#3": "MIGLTAGAYSEAEKKKKKYWYFGPLKFKWAYIRSTLTQWPSVISGKISYIPATEENTKLTKIVIKQPVPEVRRSWWNILYLFYKPRPQHSQEVYEEVEAEDGQDEETISEDVSSMELSVIAPIAQGQQEKIVAPELHIGPSQINRQDFISEGFRREKLPTRVFNSPDDRHSFVKKFKFVPDLPEDAEKKVWYSIDGEQYEAVPVEIKLLRNRLNIFQSQ"
  },
  {
    "reference": "MHKYLYIAAGGAAGSLCRYLVSGVTQRMFATSFPIGTFSVNMIGCLFFGLVTGLFEERLGLPPEMRLLILTGFMGAFTTFSTYMFESTNLIKSGQWAMTALNIGGQSILGFACIVGGLALGRLIVS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must be able to perform fluoride transmembrane transporter activity under specific conditions. The designed protein sequence is ",
    "response#1": "MPFAP",
    "response#2": "EILS",
    "response#3": "MNL"
  },
  {
    "reference": "MLKVIARRIDQFINSQSAGGVLLALSALVALVISNSPWRSYYQQFLQIPGSVKMGADWLLLSKPMLIWINDLWMAVFFFLVGLEIKRELLNGELASLKQAMLPAVAALGGMAVPALIYAAINWGEPVGLRGWGIPMATDIAFALGLLVLLGSRVPTSLKVFLTAVAIIDDLGAILVIAFFYTDNLSPTMLLAAGLGALVLLGLNRARVMAVGPYVVVGLVIWVCVLKSGIHATLAGVITALAIPLADGKGGSPLERAEHALQPWVAFLVLPVFAFANAGVSLQGVTLATLTQTVPLGIAFGLLIGKPIGVFGASWLLIRLTDAQLPDQCRWSQFFGVCVLCGVGFTMSLFIGSLAFEGADAAYEVQVKIGVLLGSLLSGAAGVALLLASRKAV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should have sodium ion transmembrane transporter activity, antiporter activity within plasma membrane to regulate regulation of pH.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. The protein should have a low Km value for 2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out). The designed protein sequence is ",
    "response#1": "GASGLLGGIL",
    "response#2": "GHVTGG",
    "response#3": "MPIGVPKVPFRNPG"
  },
  {
    "reference": "MHLSEITHPNQLHGLSIRQLEDIARQIREKHLQTVATSGGHLGPGLGVVELTIALYQTLDLDRDKVIWDVGHQAYPHKMLTGRYNNFNTLRQKNGIAGYLKRCESKFDHFGAGHASTSISAALGMALARDAQGEDYKVAAIIGDGALTGGMALEAINHAGHLPNTNLMVILNDNEMSISPNVGAISRYLNKVRLSEPVQFLSDNLEEQFKHLPFFGDVTPEMDRLKEGMKRLAVPKVGAVIEELGFKYFGPIDGHNLRELINTFKQAHKVHGPVFVHVATVKGKGYELAEQDQVGYHAQSPFNLVTGKPIPSSKPKPPGYSKVFAHTLTKLAENNPKIIGITAAMATGTGLDKLQQKLPKQYIDVGIAEQHAVTLAAGLACEGMRPVVAIYSTFLQRAYDQVIHDVCIQNLPVFFCMDRAGIVGADGPTHQGMYDIAYLRCIPNLVIMAPKDEAELQRMIVTGVNYTDGPIAMRYPRGNGLGVPLMEEGWEALPIGKGEILRNGDDLLLLGYGTMVNTAMQVAEILGEHGIEATVVNARFVKPLDTDLIVPLAKQTGKVVTLEEGCLMGGFGSAVAEALLDHNVLVPVKRFGVPDQLVDHAKPDESFADLGLTSSQIADEVLKSFFKTQEPSVIS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be optimized for thiamine pyrophosphate binding activity in order to improve thiamine biosynthetic process.2. Ideal protein should have a binding affinity for key enzymes in the 1-deoxy-D-xylulose 5- phosphate biosynthesis pathway to enhance the overall efficiency of the pathway.3. The protein must exhibit the following characteristics: Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D- xylulose-5-phosphate (DXP)..4. The protein should be able to bind Mg(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "LTFTQVFGQWLCDMAAHDERLVGITPAMREGSGMVEFEQRFPDRYYDVGIAEQHAVTFAAGMACEGAKPVVAIYSTFLQRGYDQLIHDVALQNLPVVFALDRAGLVGADGATHAGAYDIAFLRCIPNMGLACPADERECRQLLSSAYAQNHPVAVRYPRGSGAGPGRLPEPPSEGRVLIVSFGALSKQALGAAQALCDANFSATVVDPHWVVPTADSLLEFARGFDLIVTIEDNGVHGGAGSRLHYDLSQAGIDVPVRNLGVPQEFLAHGSRGEVLEDLGLDAETLAAVRDLL",
    "response#2": "PTFSKIFGDWLCEEAANDKKLMAITPAMREGSGMVRFSREYPDQYFDVAIAEQHAVTFAAGLAIGGYRPIVAIYSTFLQRAYDQVIHDIAIQNLPVLFAIDRGGIVGADGQTHQGAFDLSFLRCIPNMVIMAPSDENECRQMLHTGYHYQQGPVAVRYPRGTGTGTPGSLTFDSATEIFSKNLLDLASKYENIVGVTAAMPSGTGLDKLIEKYPNRFWDVAIAEQHAVTSMAAMAKEGFKPFIAIYSTFLQRAYDQVIHDCAIMNLNVVFAMDRAGIVGEDGETHQGVFDLSFLAPLPNFTLLAPRDEQMMQNIMEYAYLHQGPIAFRYPRGAERLPASQAGKGKKLRDGDDIAVLSIGPIGNEVIKAIEMVKEERVSIAHYDMIYLKPLDEELLHEIGQKYNRIITVENGVIKGGFGSAVLEFMADNGYTPHVKRIGVPDAFIEHGSIPELYQLCGMDAESVAKKVAAVL",
    "response#3": "PTFSKIFGDWLCEEAANDKKLMAITPAMREGSGMVRFSREYPDQYFDVAIAEQHAVTFAAGLAIGGYRPIVAIYSTFLQRAYDQVIHDIAIQNLPVLFAIDRGGIVGADGQTHQGAFDLSFLRCIPNMVIMAPSDENECRQMLHTGYHYQQGPVAVRYPRGTGTGHPQGILAGKWEKVQEGEKIAIVATGKMVQHAMIAAQKIKEEKNIDILIINATFIKPIDKELLNSLSKDGFKIVTIEDNIKKGGFGEGVLEYLNEIGHEEKIVTLAFNDKFIEHGKPDILYRINGLDAEGISWTKVFSNHLIDIANDREDIVAITAAMAGPTGLADFAKVHPSRTYDVGIAEQHAVTSAAGLALGGLHPVVAVYSTFLNRAFDQLLMDVALLKLGVTLVLDRAGITGSDGASHNGMWDLSITGIVPGIHVAAPRDARTLELALDRAVAVDDAPTVVRFPKGDAPGAKLIRPSYSEIFGQWLCDTASNDKSLMAITPAMREGSGMVTFSQQYPQQYFDVAIAEQHSVTYAAGLAISGLKPVVAIYSTFLQRGYDQLIHDVALQNLDVLFAIDRAGIVGADGPTHQGAFDLSYLRCIPNMVVMAPSNEQECLDMLTTGYQYKGPAAVRYPRGAGVGQ"
  },
  {
    "reference": "MIRASAIQRTAMLLRQLRGFSTSATLADKIKVKNPIVELDGDEMTRIIWQKIKDQLILPYLDVDLKYYDLGIESRDATDDQITIDAANAIKEYGVGVKCATITPDEARVKEFHLKKMWLSPNGTIRNILGGTVFRESIIIPCIPRLIPGWEKPIVIGRHAFGDQYKATDLVINEPGRLELRFTPASGGEAQTQKVYDYTGPGVGLAMYNTDESITGFAHASFKMALAKGLPLYMSTKNTILKKYDGRFKDIFQQIYEQDYAAEFEKQGLWYEHRLIDDMVAQMIKSKGGFVMALKNYDGDVQSDIVAQGFGSLGLMTSALMTPDGKAYEAEAAHGTVTRHYRQHQQGKETSTNSIASIFAWTRGLAQRGKLDETPDVVDFASKLEQATIDTVEVDRIMTKDLALAMGKTDRSAYVTTTEFLDAVADRLKK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The ideal protein would be one that plays a critical role in tricarboxylic acid cycle, isocitrate metabolic process, glyoxylate cycle through NAD binding, magnesium ion binding, isocitrate dehydrogenase (NADP+) activity within mitochondrion.2. Mg(2+) is required for the proper folding and stability of the protein.3. The protein must exhibit the following characteristics: Its probably critical function is the production of NADPH.; Mitochondrial IDP1 may regulate flux through the tricarboxylic acid cycle and respiration.4. The Mn(2+), NADP(+), substrate binding site should be located in a region of the protein that is accessible to the ligand.5. The desired protein should be able to perform D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH The designed protein sequence is ",
    "response#1": "MQGTGAVVLAAVYSGLKVTGIPLRDQTIVVFGAGTAGMGIADQIRDAMVADGATLEQAVSQIWPIDRPGLLFDDMDDLRDFQVPYAKNRHQLGVAVGDRVGLSDAIKIASPTILLGCSTVYGAFTKEVVEAMTASCKHPMIFPLSNPTSRMEAIPADVLAWSNGRALLATGSPVAPVEFDETTYVIGQANNVLAFPGIGLGVIVAGARLITRRMLHAAAKAIAHQANPTNPGDSLLPDVQNLRAISTTVAEAVYRAAYRHEVLYFKVLADHLPELMPVVYTPTVGEAIQRFSDEYRGQRGLFLSIDEPDEIEEAFNTLGLGPEDVDLIVCTDAEAILGIGDWGVGGIQIAVGKLALYTAGGGVDPRRCLAVSLDVGTDNEQLLADPFYLGNRHARRRGREYDEFVSRYIETAQRLFPRAILHFEDFGPANARKILDTNLYGSILSNIGSALVGGPGQKVVAPPMVYIAGEEMTRYACDLVVKSWLEPYFDLSQWEYFDLSCVNRDNTNDQVLRDAVTAGQRIGAIFKEPTITPSAIQKKAFGLKNSLGSPNGAMRAGWNGITISRDTIHIDGIELGYKRPVFFERHAVGGEYGAGWSKVGRGTLLTTYLPSDGRDPFVVDKRDLTDQHNVVVTYHNPYDNVEPLAHLFFQRCLDANITPYVVTKKTVFKWQEGFWAVMKDVFDEHYKSRFEEKGLLQACGGDLQHLISDAATMQLIRWTDGGFGMAAHNYDGDMLTDQIAQVHRSPGFITSNLVGKAPDGSLIKEFEASHGTVSDLWNDHLAGKETSLNPLGLVEAIVGALQHAAVLDAEKNPDDEHKVKARDQIFNFTTTLRTAMHNTFRYGQGTRDMSGPSGYTTEDFVRK",
    "response#2": "MQGTGAVVLAAVYSGLKVTGIPLRDQTIVVFGAGTAGMGIADQIRDAMVADGATLEQAVSQIWPIDRPGLLFDDMDDLRDFQVPYAKNRHQLGVAVGDRVGLSDAIKIASPTILLGCSTVYGAFTKEVVEAMTASCKHPMIFPLSNPTSRMEAIPADVLAWSNGRALLATGSPVAPVEFDETTYVIGQANNVLAFPGIGLGVIVAGARLITRRMLHAAAKAIAHQANPTNPGDSLLPDVQNLRAISTTVAEAVYRAASAGANAIFAPNPDSVHYRHEVLYFKVLADHLPELMPVVYTPTVGEAIQRFSDEYRGQRGLFLSIDEPDEIEEAFNTLGLGPEDVDLIVCTDAEAILGIGDWGVGGIQIAVGKLALYTAGGGVDPRRCLAVSLDVGTDNEQLLADPFYLGNRHARRRGREYDEFVSRYIETAQRLFPRAILHFEDFGPANARKILDTYNPHINGPFTPDLATPLDLEMQGTGAVVLAAVYSGLKVTGIPLRDQTIVVFGAGTAGMGIADQIRDAMVADGATLEQAVSQIWPIDRPGLLFDDMDDLRDFQVPYAKNRHQLGVAVGDRVGLSDAIKIASPTILLGCSTVYGAFTKEVVEAMTASCKHPMIFPLSNPTSRMEAIPADVLAWSNGRALLATGSPVAPVEFDETTYVIGQANNVLAFPGIGLGVIVAGARLITRRMLHAAAKAIAHQANPTNPGDSLLPDVQNLRAISTTVAEAVYRAASAGANAIFAPNPDSV",
    "response#3": "MQGTGAVVLAAVYSGLKVTGIPLRDQTIVVFGAGTAGMGIADQIRDAMVADGATLEQAVSQIWPIDRPGLLFDDMDDLRDFQVPYAKNRHQLGVAVGDRVGLSDAIKIASPTILLGCSTVYGAFTKEVVEAMTASCKHPMIFPLSNPTSRMEAIPADVLAWSNGRALLATGSPVAPVEFDETTYVIGQANNVLAFPGIGLGVIVAGARLITRRMLHAAAKAIAHQANPTNPGDSLLPDVQNLRAISTTVAEAVYRAALDLPCLVDFQKVVAPPMVYIAGEEMTRYACDLVVKSWLEPYFDLSQWEYFDLSCVNRDNTNDQVLRDAVTAGQRIGAIFKEPTITPSAIQKKAFGLKNSLGSPNGAMRAGWNGITISRDTIHIDGIELGYKRPVFFERHAVGGEYGAGWSKVGRGTLLTTYLPSDGRDPFVVDKRDLTDQHNVVVTYHNPYDNVEPLAHLFFQRCLDANITPYVVTKKTVFKWQEGFWAVMKDVFDEHYKSRFEEKGLLQACGGDLQHLISDAATMQLIRWTDGGFGMAAHNYDGDMLTDQIAQVHRSPGFITSNLVGKAPDGSLIKEFEASHGTVSDLWNDHLAGKETSLNPLGLVEAIVGALQHAAVLDAEKNPDDEHKVKARDQIFNFTTTLRTAMHNTFRYGQGTRDMSGPSGYTTEDFVRKRGEEMTAYVMDLIRSRWIEPRVDVGGWETFDLRAKNRDDTEDRVLRDVIEAGKRIKAIFKEPTVTPTADQVKRLGLRKSWGSPNGAMRRGWNGITISRDTIHIDGVELGYKKPVLFERHAVGGEYSAGYKNVGKGKLTTTFTPSEGPDAGKTVVVDEREIVDEEAAVVTYHNPYDNVHDLARFFFGRCLEAKVTPYVVTKKTVFKWQEPFWQIMRTVFDEEFKAQFVAAGVMKEGEELVHLLSDAATMKLVQWRQGGFGMAAHNYDGDVLTDELAQVHKSPGFITSNLVGVHEDGTLIKEFEASHGTVADMDEARLRGEETSLNPLGMVEGLIGAMNHAADVHNIDRDRTHAFTTKMRTVIHQLFREGKGTRDLCGPSGLTTEQFIDA"
  },
  {
    "reference": "MSRDLLRSLWLLHACSWTLLGGTSASPTPAPSSADHRLLEEKMKENGFQLHALCKFCDDKVTTCINKGLCNSKCNITSICEKKNEVCVAVWRQNDANLTLETICHDPALLLSGYKVDINPNSKKCVMKEKKAVGGLLYMCSCTESECNDEIIFAEESPTDLPKIDETAEVMLISLVPLVVITVAVIVIFYGYRIHRRRQLNKAWEKNVKPRKHKDCSDVCAIMLDDDRSDISSTCANNINHNTELLPIELDTLVGKGRFAEVYKAKLKQNTSEQFETVAVKIFPYEEYASWKTEKDIFSDINLKHENILQFLTAEERKTELGKQYWLITAFHARGNLQEYLTRHIISWEDLWKLGGSLARGIAHLHSDHTPCGRPKTPIVHRDLKSSNILVKNDLTCCLCDFGLSLRLDPTLSVDDLANSGQVGTARYMAPEVLESRMNLENVESFKQTDVYSMALVLWEITSRCNGVGEVKDYEPPFGSKVREHPCVESMKDNVLRDRGRPEIPSSWLNHQGIQKVCETLIECWDHDPEARLTAQCVAERFSELEHQDRLSGRSCSEEKIPEDGSVTTAK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis2. The protein must have high specificity towards ATP binding. The designed protein sequence is ",
    "response#1": "QLCMFCDLELSSCSGTGTCMSNCSITSTCDDSNEVCVSIWRKNEKGFSIETLCHDPSKPLYGVMLEDYNSSTCVMKEKNTTSGMAHFCSCTDEEECNDKMLFSPIK",
    "response#2": "NMCKWCEASIPMCKDSVCFSNCTLSSFCEVAEEICVAIWRKNNDTMTVQTMCHHRTLPLEGVDPNLLVNLTRRECHMVPQPSEHGDMMVCGCQGEHECNDKLIFDKGANGFR",
    "response#3": "NFCKWCEDSSPVCEDSVCLTNCSLSSFCNSIEEICVAVWRKTNDTMTVDTMCHHPALPLEGVDPGLLLNFTTRECHMVKQPAEEGAMMVCGCHGEHECNDKLIFDKGANGFA"
  },
  {
    "reference": "MSLLRCRQQTLPSLCLSVLFLACFVASMPTESLTPTPGPENSRWSLVRARVMEMVEPLVTRTRDRWRWFWGPRAIQGFVQTYYEDHLKDLGPRTQAWLQSSRDHLLNKTHSLCPRLLCRDWTQG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be engineered to have a higher affinity for a specific molecule or receptor in high-density lipoprotein particle that is critical for positive regulation of sequestering of triglyceride.2. For general function, the protein need meet that May participate in lipoprotein metabolism.3. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "FKHLRKYVYNYEAESSSGVQGTADSRSATKINCKVELEVPQICGFIMRTNQCTLKEVYGFNPEGKALMKKTKNSEEFAAAMSRYELKLAIPEGKQIVLYPDKDEPKYILNIKRGIISALLVPPETEEDQQELFLDTVYGNCSTQVTVNSRKGTVPTEMSTERNLQQCDGFQPISTSVSPLALIKGLVHPLSTLISSSQTCQYTLDPKRKHVSEAVCDEQHLFLPFSYKNKYGIMTRVTQKLSLEDTPKINSRFFSEGTNRMGLAFESTKSTSSPKQADAVLKTLQELKKLSISEQNAQRANLFNKLVTELRGLTGEAITSLLPQLIEVSSPITLQALVQCGQPQCYTHILQWLKTEKAHPLLVDIVTYLMALIPNPSTQRLQEIFNTAKEQQSRATLYALSHAVNSYFDVDHSRSPVLQDIAGYLLKQIDNECTGNEDHTFLILRVIGNMGRTMEQVMPALKSSVLSCVRSTKPSLLIQKAALQALRKMELEDEVRTILFDTFVNGVAPVEKRLAAYLLLMKNPSSSDINKIAQLLQWEQSEQVKNFVASHIANILNSEELYVQDLKVLIKNALENSQFPTIMDFRKFSRNYQISKSASLPMFDPVSVKIEGNLIFDPSSYLPRESPLLRVLLVLLASGKGESYHVYDDVQKEKTTFKGKTRSCTRHSASALGQGRVAWSLSGCSSYSLPQSCLEPTTPQCSRAWRASPYRRRNGSTAITDDTLGGTLTITLRNLQPHDAGFYQCQSLHGSEADTLRKVL",
    "response#2": "FKHLRKYVYNYEAESSSGVQGTADSRSATKINCKVELEVPQICGFIMRTNQCTLKEVYGFNPEGKALMKKTKNSEEFAAAMSRYELKLAIPEGKQIVLYPDKDEPKYILNIKRGIISALLVPPETEEDQQELFLDTVYGNCSTQVTVNSRKGTVPTEMSTERNLQQCDGFQPISTSVSPLALIKGLVHPLSTLISSSQTCQYTLDPKRKHVSEAVCDEQHLFLPFSYKNKYGIMTRVTQKLSLEDTPKINSRFFSEGTNRMGLAFESTKSTSSPKQADAVLKTLQELKKLSISEQNAQRANLFNKLVTELRGLTGEAITSLLPQLIEVSSPITLQALVQCGQPQCYTHILQWLKTEKAHPLLVDIVTYLMALIPNPSTQRLQEIFNTAKEQQSRATLYALSHAVNSYFDVDHSRSPVLQDIAGYLLKQIDNECTGNEDHTFLILRVIGNMGRTMEQVMPALKSSVLSCVRSTKPSLLIQKAALQALRKMELEDEVRTILFDTFVNGVAPVEKRLAAYLLLMKNPSSSDINKIAQLLQWEQSEQVKNFVASHI",
    "response#3": "FKHLRKYVYNYEAESSSGVQGTADSRSATKINCKVELEVPQICGFIMRTNQCTLKEVYGFNPEGKALMKKTKNSEEFAAAMSRYELKLAIPEGKQIVLYPDKDEPKYILNIKRGIISALLVPPETEEDQQELFLDTVYGNCSTQVTVNSRKGTVPTEMSTERNLQQCDGFQPISTSVSPLALIKGLVHPLSTLISSSQTCQYTLDPKRKHVSEAVCDEQHLFLPFSYKNKYGIMTRVTQKLSLEDTPKINSRFFSEGTNRMGLAFESTKSTSSPKQADAVLKTLQELKKLSISEQNAQRANLFNKLVTELRGLTGEAITSLLPQLIEVSSPITLQALVQCGQPQCYTHILQWLKTEKAHPLLVDIVTYLMALIPNPSTQRLQEIFNTAKEQQSRATLYALSHAVNSYFDVDHSRSPVLQDIAGYLLKQIDNECTGNEDHTFLILRVIGNMGRTMEQVMPALKSSVLSCVRSTKPSLLIQKAALQALRKMELEDEVRTILFDTFVNGVAPVEKRLAAYLLLMKNPSSSDINKIAQLLQWEQSEQVKNFVASHIANILNSEELYVQDLKVLIKNALENSQFPTIMDFRKFSRNYQISKSASLPMFDPVSVKIEGNLIFDPSSYLPRESVL"
  },
  {
    "reference": "MKKVTIRDFIKKKSTKEKITILTAYDYPTAKIISNTGLDSILVGDSLGMVVLGYANTLNVTMRDMISHTRAVARANPPQLIVADMPFLSYEIDTKSAVKNAGLLVKAGSDAIKLEGGEEMKDTVKAIVKAGIPVMGHIGLTPQRFLRLGGFRTIGKTKQEEDQLIKDSLELEDAGVFSLVIENTYVDIAKRITEKLNIPTICIGAGPYCDGQVLVINDLLGLSEFTPYFAKSYVNLKEIISNAINQYIIDVKNNKFPEKQHYKEKES",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for 3-methyl-2-oxobutanoate binding.2. The protein design must be focused on enhancing the efficiency of coenzyme A biosynthetic process, pantothenate biosynthetic process. The designed protein sequence is ",
    "response#1": "MRIIVGFGGGIAAYKATMLLRLFSKAGHEVVAMPTASALEFVGKPTFEALSGNPVSTSVFERVPEVNHVRQAEQADAIVVAPATADLMARLASGRADDLLSATILTTHAPVILAPAMHTQMWEHPATERNVETLKGFGYHVIEPAVGRLTGPDSGVGRMPEPEEIFEEALAAIV",
    "response#2": "MRIIVGFGGGIAAYKATMLLRLFSKAGHEVVAMPTASALEFVGKPTFEALSGNPVSTSVFERVPEVNHVRQAEQADAIVVAPATADLMARLASGRADDLLSATILTTHAPVILAPAMHTQMWEHPATERNVETLKGFGYHVIEPAVGRLTGPDSGVGRMPEPEEIFEEAVAATTPAARR",
    "response#3": "MRIIVGFGGGIAAYKATMLLRLFSKAGHEVVAMPTASALEFVGKPTFEALSGNPVSTSVFERVPEVNHVRQAEQADAIVVAPATADLMARLASGRADDLLSATILTTHAPVILAPAMHTQMWEHPATERNVETLKGFGYHVIEPAVGRLTGPDSGVGRMPEPEEIFEEAIRILEA"
  },
  {
    "reference": "MTEEFSLHPLFSPEQIQRNESAAAEREGLTMWQLMERAGQAVFRCLRQHYSIPTTVAVLCGPGNNGGDGLVVATIAQQAGYQVRLFAETGAFEKQSLEAALALNLWLENGGEIEPLSAWPEAEADVVVDALFGTGLNSRVRGIYATTIESINARKLPVLAVDIPSGLHGDTGQAMGVAVQATHTISFIGMKRGLYTADARDYCGQLWYADLGLATAIEEEVVPAAWRLESKQLRAWLPKRKEQSHKGLYGHVLVVGGQRGMAGAVRLAGEAALRSGAGKVTVVCEPGQEELVVGVPELMVLGLDTRNEEDAAELERLLTGVDVVAVGPGLGQSDWAQGLWVSVLASSKPMVVDADALNFLAQAQLKRNNWILTPHPGEAATLLDSDNKSIQANRWESAKSLQETYGGTVVLKGSGTLVQDKERVAVCTAGSAALASAGTGDVLTGITAGVWAQAEALGLSQWQVACIGVLVHGLAGECVASSFHTDEKVTRGLCASDLMTHIVRWMNPNEGNTFS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should be able to enhance the efficiency of nicotinamide nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "AVFAGSSSGAGAALHCIHGVASAGAGYIALFCDKELFSAYLASIGENAIVRVFSKDSFSPECWDVVIAGPGWGIDLERERILAMLFQTDIPLVLDADGARMFARIASKNQDSSFCRAPIILTPHPGEFSEMLPILKSVPLDPLANVSETVALVTAFAKKYGTTLALRASTTHVGLPDGSCYIYDGSTPGLGIAGSGDVLSGMIGGFLARWLAFLKETGENRESTNAAIPSDNPMTKALLGAILVHGSAGKQLWLKKGWFTPIV",
    "response#2": "AVFAGSSSGAGAALHCIHGVASAGAGYIALFCDKELFSAYLASIGENAIVRVFSKDSFSPECWDVVIAGPGWGIDLERERILAMLFQTDIPLVLDADGARMFARIASKNQDSSFCRAPIILTPHPGEFSEMLPILKSVPLDPLANVSETVALVTAFAKKYGTTLALRASTTHVGLPDGSCYIYDGSTPGLGIAGSGDVLSGMIGGFLARWLAFLKETGENRESTNAAIPSDNPMTKALLGAILVHGSAGKQLWLKKGWFTPIL",
    "response#3": "PHAKKGDFGRVLVVGGGPDYSGAPALAALAALRMGADLAMVAAPRSVASVIRSFSPNLIVKELSSDRLVPADLPFLLELAERSTCVVVGPGLGTAPETFETVEGLLERLRGKVPMVIDADAIKALASRPVLLSGAKAVITPHAGELRLLSGRKVPPPTDLRGRMAFVKEVAAELGAVVLLKAHVDVISDGKRVKINITGNPGMTVGGTGDVLSGVVATLLSWGLEPFVAACLGAFINGLAGDLAARELGYHIMATDVIERLT"
  },
  {
    "reference": "MAASQLCFVGFLEGIRGGDRLWNAKNDCRFSQRKKMRWSFYCLFTNFNYACVSQEPEKDLKFPIYSSLVVNPVGEVAISSEQKVYDVVLKQAALVEQQLRNRTVLEEKTGTTFLLNEAYDRCGQICEEYAKTFYLGTLLMTPERRRAIWAIYVWCRRTDELVDGPNASHITPSALDRWEARLEDLFAGRPYDMLDASLSDTVVNFPVDIQPFKDMIEGMRMDLKKSRYKNFDELYLYCYYVAGTVGLMSVPVMGIDPESDATTESVYNAALSLGIANQLTNILRDVGEDTRRGRVYLPQDELAEAGLSDEDIFNGKVTDRWRNFMKNQIKRARTFFQEAEKGIAELNQASRWPVLASLLLYRQILDEIEANDYNNFTKRAYVSKAKKLMAVPVAYGRSLIRPSMKKPSLVKP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should interact with enzymes and substrates within the all-trans- phytoene from geranylgeranyl diphosphate: step 1/1, Carotenoid biosynthesis pathway to enhance or regulate its activity.2. The protein must exhibit the following characteristics: Catalyzes the reaction from prephytoene diphosphate to phytoene..3. The designed protein should be involved in carotenoid biosynthetic process by localizing to plastoglobule and exhibiting geranylgeranyl-diphosphate geranylgeranyltransferase activity. The designed protein sequence is ",
    "response#1": "ELAVKLLEEKSRSFFVASAGFPSEVRERLVGLYAFCRVTDDLIDSPEVSSNPHATIDMVSDFLTLLFGPPLHPSQPDKILSSPLLPPSHPSRPTGMYPLPPPPSLSPAELVQFLTERVPVQYHFAFRLLAKLQGLIPRYPLDELLRGYTTDLIFPLSTEAVQARKTPIETTADLLDYGLCVAGSVAELLVYVSWASAPSQVPATIEEREAVLVASREMGTALQLVNIARDIKGDATEGRFYLPLSFFGLRDESKLAIPTDWTEPRPQDFDKLLSLSPSSTLPSSNASESFRFEWKTYSLPLVAYAEDLAKHSYKGIDRLPTEVQAGMRAACASYLLIGREIKVVWKGDVGERRTVAGWRAS",
    "response#2": "ELAVKLLEEKSRSFFVASAGFPSEVRERLVGLYAFCRVTDDLIDSPEVSSNPHATIDMVSDFLTLLFGPPLHPSQPDKILSSPLLPPSHPSRPTGMYPLPPPPSLSPAELVQFLTERVPVQYHFAFRLLAKLQGLIPRYPLDELLRGYTTDLIFPLSTEAVQARKTPIETTADLLDYGLCVAGSVAELLVYVSWASAPSQVPATIEEREAVLVASREMGTALQLVNIARDIKGDATEGRFYLPLSFFGLRDESKLAIPTDWTEPRPQDFDKLLSLSPSSTLPSSNASESFRFEWKTYSLPLVAYAEDLAKHSYKGIDRLPTEVQAGMRAACASYLLIGREIKVVWKGDVGERRTVAGWRR",
    "response#3": "SYRHFHLLWTLPVCGVLLVIARPFLTKLDRAKLILLPIIAFVWTTPWDNLIVRNRAWSYHRHCIWFTIGYVPIEEYFYFIIQSLISTLWGS"
  },
  {
    "reference": "MRSSFCPSLKLREAASMYFTMIAVFLVILVVALQGTAPRENYFPYKVPLDPEGTLELSWNVSYPREVVYFQILAKELKFGLLFGMSDRGEFENADLAVLWSNGSQSYFGDAWSDQKGHLHMDTQQDYKLLKAHRTTEGLYLLFKRAFSTCDPKDYFIEDGTVHLIYGILDKPVHSLQAINVSAIQRGLQRVQLLKPNITMPNLPTDMKTMEIRTPDVVIPSQETTYWCYMTELPEDFPRHHIVMYESVITAGNEAIVHHMEVFQCAAQFDSFPRYSGPCDSKMKPERLNYCRHVLAAWAMGAQVRCGREAGLAFGGPGSSRYLRLEVHYHNPLVFKGRHDSSGIRLYYTATLRQYDAGIMELGLVYTPMMAIPPGETQFLLTGYCTDKCTQVALPPSGIHIFASQLHTHLTGRKVVTVLSREGKERMIVNADNHYSPHFQGDALMTTCTYNTEDRSRATVGGFGIMEEMCVNYVHYYPQTQLELCKSTVDSGYLHRYFNLVNRFNDEEICMCPLASVSQQFSAIPWNSFNRDVLKSLYDFAPISMHCNKSSAVRFPVRLNGLPRMRKMAAPCTVHA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be involved in fear response, leukocyte mediated immunity, regulation of vascular endothelial cell proliferation by localizing to extracellular space, chromaffin granule membrane and exhibiting copper ion binding, dopamine beta-monooxygenase activity.2. For general function, the protein need meet that Conversion of dopamine to noradrenaline.3. The protein design should consider the endogenous proteins and enzymes present in the Catecholamine biosynthesis pathway to avoid interference with native metabolic processes.4. The presence of DOMON domains is critical for the interaction of the designed protein with its intended targets.5. The protein design should incorporate a binding pocket that specifically recognizes Cu(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "WFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLERFSGYREDNIPQLEDVSRFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCCHELLGHVPMLADRTFAQFSQDIGLASLGASDEEIEKLSTLYWFTVEFGLCKQNGEVKAYGAGLLSSYGELLHCLSEEPEIRAFDPEAAAVQPYQDQTYQSVYFVSESFSDAKDKLRSYASRIQRPFSVKFDPL",
    "response#2": "PWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLERFSGYREDNIPQLEDVSRFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCCHELLGHVPMLADRTFAQFSQDIGLASLGASDEEIEKLSTLYWFTVEFGLCKQNGEVKAYGAGLLSSYGELLHCLSEEPEIRAFDPEAAAVQPYQDQTYQSVYFVSESFSDAKDKLRSYASRIQRPFSVKFDPYTLAIDVLDSPQAVRRSLEGVQDELDTLAHAL",
    "response#3": "YDAGIMELGLVYTPVMAIPPGETGFLLKGYCTDKCTQAALPPAGIHIFASQLHTHLTGRKVVTILSRDGREREVVNADNHYSPHFQEIRMLKKMVSVFPGDVLVTTCTYNTEDRNQATVGGFSIMEEMCVNYVHYYPQTQLELCKSAVDSGYLQRYL"
  },
  {
    "reference": "MAQALLVPPGPESFRLFTRESLAAIEKRAAEEKAKKPKKEQDIDDENKPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYVSKKTFVVLNKGKAIFRFSATSALYILTPLNPVRKIAIKILVHSLFSMLIMCTILTNCVFMTLSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFSVIVMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPSDSAFEINTTSYFNGTMDSNGTFVNVTMSTFNWKDYIADDSHFYVLDGQKDPLLCGNGSDAGQCPEGYICVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDYWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLVNLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEEAQAVAAASAASRDFSGIGGLGELLESSSEASKLSSKSAKEWRNRRKKRRQREHLEGNHRPEGDRFPKSESEDSVKRRSFLFSLDGNPLSGDKKLCSPHQSLLSIRGSLFSPRRNSKTSIFSFRGRAKDVGSENDFADDEHSTFEDSESRRDSLFVPHRPGERRNSNGTTTETEVRKRRLSSYQISMEMLEDSSGRQRAMSIASILTNTMEELEESRQKCPPCWYRFANVFLIWDCCDSWLKVKHLVNLIVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLTVGNLVFTGIFTAEMVLKIIAMDPY",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should contain a Helical; Name=S6 of repeat I, Helical; Name=S5 of repeat II, Helical; Name=S2 of repeat II, Helical; Name=S6 of repeat II, Helical; Name=S1 of repeat I, Helical; Name=S5 of repeat III, Helical; Name=S1 of repeat IV, Helical; Name=S4 of repeat II, Helical; Name=S6 of repeat IV, Helical; Name=S1 of repeat II, Helical; Name=S6 of repeat III, Helical; Name=S2 of repeat III, Helical; Name=S2 of repeat IV, Helical; Name=S3 of repeat I, Helical; Name=S1 of repeat III, Helical; Name=S5 of repeat IV, Helical; Name=S2 of repeat I, Helical; Name=S3 of repeat II membrane-spanning region.2. The protein design should emphasize Polar residues compositional bias.3. The ideal protein would be one that plays a critical role in response to pyrethroid, neuronal action potential, behavioral response to pain, membrane depolarization during action potential, regulation of ion transmembrane transport through sodium ion binding, voltage-gated ion channel activity, sodium channel activity, voltage-gated sodium channel activity, calmodulin binding within axon, sarcoplasm, voltage-gated sodium channel complex, basal plasma membrane. The designed protein sequence is ",
    "response#1": "LAFQSIGVVYGDVGTSPLYVYSSTFPDGVKHPDDLVGVLSLMLYTLILIPMVKYVFIVLYANDNGDGGTFALYSLISRHAKIRMIPNDQTEDANVSNYSIEAPSSQLRRAEWVKQKLESSNAAKIALFTITILGTSMVMGDGTLTPAISVLSAVSGIREKAPSLTQLQVVWISVPILIVLFSVQRFGTDKVGYSFAPVISVWFVLIAGIGAYNLAVHEITILRAFNPMYIIDYFRRNGKEAWVSLGGAVLCITGTEAMFADLGHFNIRAIQLSFTCVLFPSVALCYMGQAAYLRKFPEDVGDTFYKSLPAPLFWPVFVVAIMAAIIASQAMLSGAFAILSKALPLGCFPRVEVVHTSNKYEGQVYIPEVNFLIGVASVAITVAFQTTANIGNAYGICVVMVFSITTHLMTVVMLLIWKVRLPFIAAFYVVFTFTEFLYLSSILSKFAEGGYLPFCFSLVLMALMATWHYVHVKRYWYELDHIVPPDEPGVGLLYTELVQGIPPVFPRLVDKIPSVHAVFVFMSIKHLPIPRVAPAERFIFQRVGPDAGHRIFRCVARYGYTDPLEGAKEFAAFLLDRLKVFVYEEAVFACQCAEDGGGGGGGDDDGVLRRAEEMAAEEKRLIDAEAERGLVYLMGEANVEAAPGSSLMKQIVVNYVYTRLRKNLREEHKALSIPKDQLLKVGITYEL",
    "response#2": "QLSLTHGLSRTSMRPRSSRGSIFTFRRRDQGSEADFADDENSTAGESESHRTSLLVPWPLRHPSAQGQPGPGASAPGYVLNGKRNSTVDCNGVVSLLGAGDAEATSPGSYLLRPMVLDRPPDTTTPSEEPGGPQMLTPQAPCADGFEEPGARQRALSAVNDTKPDFPIVYCNESFCKISGYNRAEVMQKSCRYVCGFMYGELTDKETVGRLEYTLENQQQDQFEILLYKKNNLQCGCALSQFGKAQTQETPLWLLLQVAPIRNERDLVVLFLLTFRDDFPIVYCNESFCKISGYNRAEVMQKSCRYVCGFMYGELTDKETVGRLEYTLENQQQDQFEILLYKKNNLQCGCALSQFGKAQTQETPLWLLLQVAPIRNERDLVVLFLLTFRDGTEEEEEGTFLENIVRRSNDTNFVLGNAQIVDWPIVYSNDGFCKLSGYHRAEVMQKSSACSFMYGELTDKDTVEKVRQTFENYEMNSFEILMYKKNRTPVWFFVKIAPIRNEQDKVVLFLCTFSDITAFKGHEAPSKHSLLAFQSIGVVYGDVGTSPLYVYSSTFPDGVKHPDDLVGVLSLMLYTLILIPMVKYVFIVLYANDNGDGGTFALYSLISRHAKIRMIPNDQTEDANVSNYSIEAPSSQLRRAEWVKQKLESSNAAKIALFTITILGTSMVMGDGTLTPAISVLSAVSGIREKAPSLTQLQVVWISVPILIVLFSVQRFGTDKVGYSFAPVISVWFVLIAGIGAYNLAVHEITILRAFNPMYIIDYFRRNGKEAWVSLGGAVLCITGTEAMFADLGHFNIRAIQLSFTCVLFPSVALCYMGQAAYLRKFPEDVGDTFYKSLPAPLFWPVFVVAIMAAIIASQAMLSGAFAILSKALPLGCFPRVEVVHTSNKYEGQVYIPEVNFLIGVASVAITVAFQTTANIGNAYGICVVMVFSITTHLMTVVMLLIWKVRLPFIAAFYVVFTFTEFLYLSSILSKFAEGGYLPFCFSLVLMALMATWHYVHVKRYWYELDHIVPPDE",
    "response#3": "HSLFSMFIMITILPNCVVMTMSDPPPWPIHVENTFTGINTFESLIKMLARGFCIDDFTFLRDPWNWLDFSVIMMAYLTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPQPFNDTNTTWYGNDTWYSNDTWNSNDTWSSNDMWNSHESMASNYTFDWDAYINDEGNFYFLEGAKDALLCGNSSDAGHCPEGYKCIKTGRNPNYGYTSHDTFSWAFLALFRLMIQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVTMAYAEQNELTTKNPMQYRRKLAFQSVGIIYGDIGTSPLYVYSSTFPDGIGHRDDLVGVLSLILYTLIIIPMLKYVFIVLYANDNGDGGTFALYSLISRYAKIRMIPNQQAEDAMVSNYSIEAPSSQLRRAQWVKHKLESSRAAKMALFFLTILGTSMVMGDGTLTPAISVLSAVSGIREKAPNLTQTQVVLISVAILFMLFSVQRFGTDKVGYTFAPIISVWFLLIAGIGLYNLVVHEITILKAFNPWYIVQYFRRNGKKGWVSLGGVVLCVTGTEGMFADLGHFNIRAVQISFNCILFPSVALCYIGQAAYLRKFPENVSDTFYKSIPGKYRDRLNFGPLFWPTFIVAILAAIIASQAMLSGAFAILSKALSLGCLPRVRVIHTSKKYEGQVYIPEVNFMMGLASIIVTIAFRTTTSIGNAYGICVVTTFMVTTHLMTVVMLLIWKKHLVFILLFYCVFGFTEVVYLSSILSKFVDGGYLPFCFAMVLMTMMATWHYVHVRRYWYELDHIVPTAE"
  },
  {
    "reference": "MVYQSRSFQVEVSGLHQNEVTNQNNYPIRSSGSVFITIPFSRFNEELQRINRLGGKIVNIQPLNLQINEN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that Linker polypeptides determine the state of aggregation and the location of the disk-shaped phycobiliprotein units within the phycobilisome and modulate their spectroscopic properties in order to mediate a directed and optimal energy transfer. The designed protein sequence is ",
    "response#1": "MPFGPASRLGVSLFDETPPVEWVPGRSQEEAETIIRAIYRQVLGNAYVMESERLAVPESQFKRGELSVREFVRAVAKSELYRSRFFTSCARYRAIELNFRHLLGRPPLDLEEMRSHSTILDTQGFEAEIDSYIDGDEYQSTFGENIVPYIRGYKTEALQSMVQFTHTFQLVRGASSSSS",
    "response#2": "TAEEVRSVIWSAYRQVLGNEHLFESERLSSAESLLQQAQISVRDFVRAIAQSELYRQKFFYSNSQVRFIELNYKHLLGRAPYDESEIAYHVDIYTSQGYEAEINSYIDSVEYQQNFGDSIVPYYF",
    "response#3": "MPVTVAASRLGTAAFDQSPVELRANYSRDDAQTVIRAVYRQVLGNDYVMSSERLTAAESLFTNGFISVRDFVRAVAQSELYKEKFLYNNFQTRVIELNFKHLLGRAPYDEAEVIEHLDRYQNEGFEADINSYIDSAEYTENFGDNIVPYIRSYVVQTGHRTVGFTRMFSLQRGYANSDC"
  },
  {
    "reference": "MERIVIALAIISIVKGDQICIGYHANNSTEQVDTIMEKNVTVTHAQDILEKEHNGKLCSLKGVRPLILKDCSVAGWLLGNPMCDEFLNVPEWSYIVEKDNPVNGLCYPGDFNDYEELKHLMSSTNHFEKIQIIPRSSWSNHDASSGVSSACPYNGRSSFFRNVVWLIKKNNAYPTIKRTYNNTNVEDLLILWGIHHPNDAAEQTKLYQNSNTYVSVGTSTLNQRSIPEIAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVRGLFGAIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAADKESTQKAIDGITNKVNSIIDKMNTQFEAVGKEFNNLERRIENLNKKMEDGFLDVWTYNAELLVLMENERTLDFHDSNVKNLYDKVRLQLRDNAKELGNGCFEFYHKCDNECMESVRNGTYDYPQYSEESRLNREEIDGVKLESMGTYQILSIYSTVASSLALAIMVAGLSFWMCSNGSLQCRICI",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Several trimers are required to form a competent fusion pore.; Class I viral fusion protein; This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway2. The designed protein should have host cell surface receptor binding within virion membrane, host cell plasma membrane, apical plasma membrane to regulate virion attachment to host cell. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWKEAKTTLFCASDAKAYEKEMHNVWTTHACVPTDPNPQEVALENVTENFNIWKNDMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCSNAC",
    "response#2": "LWVTIYYGVPVWKEANTTLFCASNAKAYDTEVHNVWATHACVPTDPNPQEVRLENVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDANTTNTNANSTNNSSLGTMEKGEIKNCSFNITTNLRDKVQKEYALFYNLDVVPIKGEDNTSYRLISCNTSVITQACPKVSFEPIPIHYCTPAGFAILQCNDKKFNGSGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEKEVVIRSENFTNNAKTIIVQLNESVVINCTRPNNNTRKSIPIGPGRAFYATGEIIGDIRQAHCNVSTKAWKEALQQVAIKLSEQFGNKTIVFNQSSGGDPEVVMHSFNCRGEFFYCNTTGLFNNTWGFNSTWDAINVPENDTITLPCRIKQIVNMWQEVGKAMYAPPIRGRLNCSSNITGLLLTRDGGNTTNNTEVFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKR",
    "response#3": "GNATIGNDTQEMKNCSFNATTEIRDKKQKAYALFYKLDIVSLNNNSEYRLINCNTSAITQACPKVSFEPIPIHFCAPAGFAILKCRDEEFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEDKVKIRSENITNNAKVIIVQLVSPVKINCSRPNNNTRTSIRIGPGQSFHATGGIIGDIRQAHCNVSRKEWNSALRQVAAQLGTYFGNKTIIFTKSSGGDLEITTHSFNCGGEFFYCNTSDLFDSAWNSSTWNNNSSQESDSTGANDTITLQCRIKQIINMWQRVGQAMYAPPIQGVIRCESNITGLLLTRDGGNNSTTNETFRPGGGDMRDNWRSELYKYKVVEIEPLGVAPTRAKRRVVEREKR"
  },
  {
    "reference": "MGEKKPEPLDFVKDFQEYLTQQTHHVNMISGSVSGDKEAEALQGAGTDGDQNGLDHPSVEVSLDENSGMLVDGFERTFDGKLKCRYCNYASKGTARLIEHIRIHTGEKPHRCHLCPFASAYERHLEAHMRSHTGEKPYKCELCSFRCSDRSNLSHHRRRKHKMVPIKGTRSSLSSKKMWGVLQKKTSNLGYSRRALINLSPPSMVVQKPDYLNDFTHEIPNIQTDSYESMAKTTPTGGLPRDPQELMVDNPLNQLSTLAGQLSSLPPENQNPASPDVVPCPDEKPFMIQQPSTQAVVSAVSASIPQSSSPTSPEPRPSHSQRNYSPVAGPSSEPSAHTSTPSIGNSQPSTPAPALPVQDPQLLHHCQHCDMYFADNILYTIHMGCHGYENPFQCNICGCKCKNKYDFACHFARGQHNQH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that contributes to negative regulation of transcription by RNA polymerase II, regulation of transcription by RNA polymerase II by functioning in protein-containing complex with DNA-binding transcription factor activity, RNA polymerase II cis-regulatory region sequence-specific DNA binding, zinc ion binding. The designed protein sequence is ",
    "response#1": "FGVQETAIISQEVHNFIMDQGWSEYQFCNQIWAGKCPKTIRMFYSNLYKKLSHRDAKSIYHHVRRAYS",
    "response#2": "RNLLAAFKEIGRMADHMNLPQTVQDRANELFRFMDDKKSTKGRSVDGMVAAALYIACRQEHLSRTFKEIAALTNVPKKEISRCYKIMKEC",
    "response#3": "FGVQETAIISQEVHNFIMDQGWSEYQFCNQIWAGKCPKTIRMFYSNLYKKLSHRDAKSIYHHVRRAYR"
  },
  {
    "reference": "MFDSIERALQALKNGEMIIVCDDEDRENEGDLLAITEWMNDDTINFMAKYGRGLICCPIDHSIAQQAGIDAMVHHNTDPHATAFTVSIDHVTSTTGISAIERTKTARALIDPNMSSHTFNQPGHMFPLIANPHGVLGRRGHTEAAVDLAKLTNARPAGVICEIMNEDGTMAKGEKLQLFKEKHNLCMITIEALVAYIKTNETLITEEAKVKLPTRYGDFDMYGFTSDIDRSELLAIVKGDIHEQMNVRIHSACATGDIFHSARCDCGEQLEAAMSYIQDNGGMIIYLPQEGRGIGLMNKLKAYELIERGYNTVSANEALGFKPDMRDYDNAAQILKYFGIQTVNLLTNNPEKFEGLAHYGITMTTRIPLVTELNPHNYEYMQVKKNKMRHLI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must have high specificity towards GTP cyclohydrolase II activity. The designed protein sequence is ",
    "response#1": "GVDMPTEWGHFDLIAFKQSNTDDTHLALVKGTWEPGEPVLVRVHSSCVTGDIFGSCRCDCGGQLHAAMEMVEREGKGVVLYMFQEGRGIGLINKLKAYKLQEMGRDTVEANLDLGLPMDARDYGVGAQILRDLNVHKLRLISNNPKKRAGLMGYGLEIVDTVG",
    "response#2": "GVDMPTEWGHFDLIAFKQSNTDDTHLALVKGTWEPGEPVLVRVHSSCVTGDIFGSCRCDCGGQLHAAMEMVEREGKGVVLYMFQEGRGIGLINKLKAYKLQEMGRDTVEANLDLGLPMDARDYGVGAQILRDLNVHKLRLISNNPKKRAGLMGYGLEIVDTVI",
    "response#3": "GVDMPTEWGHFDLIAFKQSNTDDTHLALVKGTWEPGEPVLVRVHSSCVTGDIFGSCRCDCGGQLHAAMEMVEREGKGVVLYMFQEGRGIGLINKLKAYKLQEMGRDTVEANLDLGLPMDARDYGVGAQILRDLNVHKLRLISNNPKKRAGLMGYGLEIVDTVL"
  },
  {
    "reference": "MAGVNGSHASPSPAKQRAKMDDIVVVAPGTQSSRNVSNDPDVIKLQEIPTFQPLLKGLLSGQTSPTNTKLEKLDPHQVLQLCLRYQDHLHQCAEAVAFDQNALVKRIKEMDLSVETLYSFMQERQKKYAKYAEQIQKVNEMSAILRRIQMGIDQTVPLMERLNSMLPESEQLEPFSMKPDREIKS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a specific function in lysosome localization and should be localized to BORC complex, synaptic vesicle membrane in order to carry out this function efficiently.2. The protein must exhibit the following characteristics: As part of a BORC-like complex may play a role in lysosomes movement and localization at the cell periphery. The designed protein sequence is ",
    "response#1": "PPIDPDVLRDLERLSRELGGRVDRLLCGLGGAVQELTALSVGCIQTYRDAVDSLGEAVDMSIKGMYTLLARCEELERALQPVQGLARQVRDIRRTLEVLG",
    "response#2": "PPIDPDVLRDLERLSRELGGRVDRLLCGLGGAVQELTALSVGCIQTYRDAVDSLGEAVDMSIKGMYTLLARCEELERALQPVQGLARQVRDIRRTLEVLS",
    "response#3": "PRSIEVKYIGVKSAYVSYDAEKRTIYLNITNTLNITNNNYYSVEVENITAQVQFSKTVIGKARLNNITNIGPLDMKQIDYTVPTVIAEEMSYMYDFCTLLSIKVHNIVLMMQVTVTTAYFGHSEQISQERYQYVDCGRNTC"
  },
  {
    "reference": "MINLAYDDNGTGDPVVFIAGRGGAGRTWHPHQVPAFLAAGYRCITFDNRGIGATENAEGFTTQTMVADTAALIETLDIAPARVVGVSMGAFIAQELMVVAPELVSSAVLMATRGRLDRARQFFNKAEAELYDSGVQLPPTYDARARLLENFSRKTLNDDVAVGDWIAMFSMWPIKSTPGLRCQLDCAPQTNRLPAYRNIAAPVLVIGFADDVVTPPYLGREVADALPNGRYLQIPDAGHLGFFERPEAVNTAMLKFFASVKA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must be able to hydrolase activity.2. The protein design should prioritize the formation of strand-helix-turn-helix-strand-helix-helix-strand-helix-helix-strand-helix-helix-helix-helix elements.3. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate binding.4. The active site active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "PLYGELKTQLATAASLRMDKHRSNSVGPHDLYFTLLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAGSQRETVQAVTDGALIDITDMREWREEN",
    "response#2": "RGLLIVLSGPSGVGKGTVREAVFKDPETSFDYSISMTTRLPREGEQDGVDYYFRSREVFEQAIKDGKMLEYAEYVGNYYGTPLEYVEEKLAAGVDIFLEIEVQGAMQVRKAMPEGIFIFLTPPDLSELKNRIIGRGTESMEVVEERMETAKKEIEMMASYDYAVVNDVVANAVQKIKGIVETER",
    "response#3": "HFDAPAGMLIPDSVATTVSTAFRRSGASTVGAHPSARRSAAVLDAAREAVADLVNADPGGVVLGADRAVLLSLLAEASSSRAGLGYEVIVSRLDDEANIAPWLRAAHRYGAKVKWAEVDIETGELPTWQWESLISKSTRLVAVNSASGTLGGVTDLRAMTKLVHDVGALVVVDHSAAAPYRLLDIRETDADVVTVNAHAWGGPPIGAMVFRDPSVMNSFGSVSTNPYATGPARLEIGVHQFGLLAGVVASIEYLAALDESARGSRRERLAVSMQSADAYLNRVFDYLMVSLRSLPLVMLIGRPEAQIPVVSFAVHKVPADRVVQRLADNGILAIANTGSRVLDVLGVNDVGGAVTVGLAHYSTMAEVDQA"
  },
  {
    "reference": "MLLKNKNILVGVTGSIAIYKALELIRLYIKAGAAVRVVMTDAAKKFINPITFEAISQNKILDESSENWDKSQDYNHIDIAKWSDIFIIAPASANTINKLSSGIADNLLLQTALAYTKAIVLSPAANTNMIENSITKKSLENLTKLGFEILGTQTKELICKDVGNGAMAEPIDIFHKTCRELLRNFYWENRKVVLSGGGTIEKIDDVRYISNFSSGKMASSLATALYYLGADVSLVASRGLEYAPKGINRIQIFSTLEFKENLEKELKLNQNSKKTYLFMVAAISDYVPSLKVDGKIKKETLGETWNLKLKKNIDILASIEKNNIISVGFKAEMDKVNAKTNAENMLINKNLDAVCLNILDENNKFGDENNIIELIFKDESKNFSGNKLDISFSILENLEKKFEAHE",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..2. The designed protein should have a high specificity for CTP binding, with minimal off-target effects.3. The protein design should take into account the physiological conditions of the coenzyme A biosynthesis pathway.4. The protein must exhibit metal ion binding, phosphopantothenoylcysteine decarboxylase activity. The designed protein sequence is ",
    "response#1": "MEIVVGVTGGIAAYKTVHLVRLLIGDGHNVHVVPTPDALRFVGLPTWEAISRNRVTTSVHEDVPQVRHVALGQAAELIIVAPATANTLASMAAGLAHDLLGTTLLASRAPVLVAPAMHTEMWQHPATQANMATLRARGVQVVGPDDGPLTGGDSGPGRMSEPEAIHAVVDRVLG",
    "response#2": "MEIVVGVTGGIAAYKTVHLVRLLIGDGHNVHVVPTPDALRFVGLPTWEAISRNRVTTSVHEDVPQVRHVALGQAAELIIVAPATANTLASMAAGLAHDLLGTTLLASRAPVLVAPAMHTEMWQHPATQANMATLRARGVQVVGPDDGPLTGGDSGPGRMSEPEAIHRAIRRVLEG",
    "response#3": "MEIVVGVTGGIAAYKTVHLVRLLIGDGHNVHVVPTPDALRFVGLPTWEAISRNRVTTSVHEDVPQVRHVALGQAAELIIVAPATANTLASMAAGLAHDLLGTTLLASRAPVLVAPAMHTEMWQHPATQANMATLRARGVQVVGPDDGPLTGGDSGPGRMSEPEAIHGAARAALHGDPLAGLTIAVSAGGTREPIDPVRFIGNRSSGRQGVALAVAAAERGAQVLLASANIDGDVLAEARRPGVEITQAGSAAELSELMAQLATRADVVIMAAAVADYRVVEVSDGKLTKESGGVPTIRLTENEDILAGLASRRRPGQTIVGFAAETARGDELLERGRRKRRRKGVDLLAVNEVGWTTGFGVADNKLVILRTEADRVEMVLEGKTVLITAGATREFVDPVRMLTNRSTGKMGFALAWEAFARGARVILIHGPTELTPPAEVEAHAVVSAEEMYAAVEQRFPEADIFISAAAVADYRPVERAAHKLKKGKQPLALTLERTPDILQAMSRQRKAHQRLVGFAVETDSPRENALKKLENKNLDVVVLNNPLESGAGFATDTNRVTLLGADGLTLAEVPVTAKSEVADALLDVVPR"
  },
  {
    "reference": "MSHSPTPLAAIVLAAGKGTRMKSQKAKVLHEVGGRPLAWFPTRRALEIGANPVVAVVGHQAEAVEAALAATLPGAPLRFAVQREQLGTAHAVLSAREALGRYQGAVLILSGDTPLLRAETLSRVVAARAGATLSLATMRLADPHGYGRIVRDPAGTPARVVEEKDATDAERALDEVNAGLYCADAAFLWEALSKVGSANAQREFYLTDLVAMAARAGGVVAVPVPPEEASGVNDREELARAGRVLLRRRASELMRSGVTIEDPERFDCDEGVEIGADAVIEPNVRLKGRTRIGAGCRLGAGAILTDAVLADGVTVKPYTVIEEATVAARAILGPFSRLRPGSDIGEEAHVGNFVETKKARLGKGAKANHLTYLGDATIGAGANVGAGTITCNYDGEKKHPTTIGEGAFIGSDSILVAPIEIGAGAYVAAGSTLTESVPPGALALGRAKQVTKEGWVARRKAEAQNKGAAEAAPAPSPADSPRGGRAS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain..2. The protein design should consider the endogenous proteins and enzymes present in the Bacterial outer membrane biogenesis pathway to avoid interference with native metabolic processes.3. The protein should be able to bind Mg(2+) ligand in a variety of conditions4. A protein that localizes to cytoplasm and can perform glucosamine-1-phosphate N-acetyltransferase activity with high specificity and efficiency.5. The presence of Mg(2+) is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "VIVLAAGMGTRMKSSLPKVMHPLAGRPMVSHLLDTVSGLSPDRMVVVVGPDMELVGKTVAPFPTVIQADRLGTGHAVLQAKAALGQFDGDVLVLYGDTPLITRATLERMLAERRGPRDPAVVVLGFKPQDPGFGRLLRQGEELLAVVEHKEATPEQRCIREIYSGIMAVPTARLRQWLARLDNQNAQGEYYLTDIVAMAVADGVPVAVAQPESALEVAGVNNRLQLAELERAWQLRQAQALLEAXGVAVQDAXSXFVDXRXGVTIIDPASTWVDAGVRIGRDAVLRPGVQLLGSTVIGEDAEIGPDSTLTDVRVGDGAKVVRTHGEGATIGPNASVGPFAYLRPGTILGEAGKLGAFVETKNADIGAHSKVPHLTYVGDATIGEHSNIGASSVFVNYDGVKKHHTVVGSHVRTGSDTMFVAPVTVGDGAYTAAGTVLRRNVPPGALAVSGGPQRHIRRWKKPSKK",
    "response#2": "VILAAGKGTRMRSKLPKVLHPIAGKPMVQHVIDNSKALGAKSTNLVYGHGGEQLKVCLSHNDVNWVLQEEQLGTGHAVAVAKQHISDDDTVLILYGDVPLTKQSTLEKLLAVTPAKGLAVLTVELADPTGYGRIVRKASGQVTGIVEHKDATDAQREITEINTGILVANGADLKRWLSALSNDNAQGEYYLTDVIAMAAKDGVTVSTAQPNRLSEVEGVNNRLQLSALERVYQYQQAENLMLAGVSLQDPATAYIEEGVTVGPDTEIGPSVTLAAGTVVGKGCTIGQGSVLHASTVADGTVIKPYSVLEEARVGERNVIGPFSRLRPGTELAEDVHLGNFVETKKARIGKGSKANHLTYLGDAVIGSGCNVGAGTITCNYDGVNKHLTELGDGVFIGSDTQLVAPVKVGDGSYVGAGTTVTKNVPPGSLAVSRTPQRTLEGWKRPVKKK",
    "response#3": "VILAAGKGTRMRSKLPKVLHPIAGKPMVQHVIDNSKALGAKSTNLVYGHGGEQLKVCLSHNDVNWVLQEEQLGTGHAVAVAKQHISDDDTVLILYGDVPLTKQSTLEKLLAVTPAKGLAVLTIELTDPTGYGRIVRDKDGNVLKIVEQKDANEEEKSIPEINTGIYAFDASILREALKQLSTDNAQGEYYLTDVIALARAENKKVGASICKDASQTEGANDRAQLAALGRELNSKICQAHMRAGVTLIDPDSITIDDTVLLQQDVIVEPQTHIRGSSIIGSGSRIGPGSLIENSHIGKNTSVLYSVISDSMVADNTRIGPYAHLRGDSQVGSHCRIGNFVELKKATVGDRSNAAHLSYLGDATLGEKVNIGAGTITANYDGVKKHKTKIGDRSKTGSNSVLVAPVTLGEDVTVAAGSVVTKNVEDDSLVIGRARGAPARVIEGWTQKRRPGSKAAEAAKKASE"
  },
  {
    "reference": "MKLRTGLIGWRGMVGSVLMDRMKEEKDFDHIDPLFYSTSQAGQKAPDVGYDVPALADAYDLTSLKNMDAVICCQGGAYTEKVYPELRKAGWKGYWIDAASTLRMAPDSEIILDPVNRAVLDEALSKGVKTFAGGNCTVSLMLMGLGGLFENDAVEWLTSMTYQSASGAGARNMRELLLQMRELGAAAGADLDNPASAILDIDRRITAKLNDGDFPVDNFGAPLAGSLIPWIDKAVDDGQTKEEWKGFAETNKILRTKTPIEVDGICVRVGAMRCHSQALTIKLKKDIPLGDIEDMIQNGTDWSDLINNNKEDTLEYLSPAAVSGTLRVPVGRVRKMRMGGEYLSAFTVGDQLLWGAAEPLRRMMRIIVER",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein design must be able to perform aspartate-semialdehyde dehydrogenase activity, NADP binding, NAD binding, protein dimerization activity in order to contribute to lysine biosynthetic process via diaminopimelate, 'de novo' L-methionine biosynthetic process, isoleucine biosynthetic process.2. The designed protein should have a binding site that is specific to the target phosphate, NADP(+), substrate ligand.3. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate.4. The designed protein is the optimization of the Proton acceptor, Acyl-thioester intermediate to achieve high catalytic efficiency for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.5. A protein should be able to manipulate the activity of enzymes in the Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5, L-threonine biosynthesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "MTDIAVVGATGSVGREILEILEERNFPVKNIFCLASERSEGEIINFRDTDIKVIDLKKFNFTDIDLALFSPGATISKKFAPVAASKGCLVIDNTSYFRMEKDIPLIVPEINSSELDSLKPIHDAVGIERINVCTYQAVSGTGKDAIQELATQTANLLNTKDLKAEVYPKQIAFNVLPHIDVFQENGYTKEEMKMVWETHKIMDDESILVNPTAVRVPVFFGHSEALHIETCDKMTADQCRELLASSPGIEIMDERKDGGYPTAVTEGANSDTVYVGRIREDISHPRGLDLWVVADNVRNGIANYILKPIYDAVGIARINVCTYQSVSGTGKEAIQELAQQTAAILNGKPVEKAVYPKQIAFNVLPHIDVFQDNGYTKEEMKMVWETQKIMGDDSILVNATTARVPVFYGHSEAVHIETRRKITAAEARKLLEKMPGVVVVDERADGGYPTAVTEAAGRDPVYVGRIREDISHPLGLNLWVVSDNVRGLFREGLVEWVSAQTYQAASGAGAKNMRELISQMGAIRDAVAPELADARSNILDIDRKVAEAMRADAFPTNEFGHALAGSLLPWIDTQLENGQSREEWKGQAETNKILGRSESPIAIDGTCVRIGAMRCHSQALTIKLNKEVAMSDVEQIIASANDWVDVVPNDRDETLKRLTPTAVTGTLGVPVGRLRKLNMGSQYLSAFTVGDQLLGE",
    "response#2": "MSYTLAVVGATGQVGVVMRRLLEERAFPADKVRFFASARSAGTTLPFRGEDIVVEDTAATPDEDLQGIDIALFSAGGTLSKEQAPRFAAAGAVVVDNSSAWRKDPDVPLVVSEVNPGDLANRPKGIIANPNCTTMSLAVMPLKPLHDKFKVKRVVVSTYQSVTGAGKRGIDQLFAEIKGDKILNPKFPHQIAFNCIPHIDNFYEDGYSKEEHKIMKETIKIIGDNSIKITATCVRVPVIGGHSEAVNIEFEKDFEIEEIRDVLNNSPGIVLYDDPYNNAYPMPLISHEKDEVFVGRIRRDYSSENALNLWIVADNLRKGAALNAVQIAELVGLFREGLVEWVSAQTYQAASGAGAKNMRELISQMGAIRDAVAPELADARSNILDIDRKVAEAMRADAFPTNEFGHALAGSLLPWIDTQLENGQSREEWKGQAETNKILGRSESPIAIDGTCVRIGAMRCHSQALTIKLNKEVAMSDVEQIIASANDWVDVVPNDRDETLKRLTPTAVTGTLGVPVGRLRKLNMGSQYLSAFTVGDQLLG",
    "response#3": "MTDIAILGATGVVGESLLSILAEQSFPVGKLYPLASSRSKGETVTFKGQQLLVEDAADFDFRKAQIAFFSAGASVSADYAPIAAKAGCIVIDNTSQFRNDADIPLVVPEVNPAALAHLKPIYDAVGISRINIATYQSVSGTGKAALEALTAQSTAILANKEPQLAVYPIPIGFNLIPHIDIFEENGYTREEMKIVRETRKILEDELICVNPTAVRVPVYVGHSEAVHIETKTKLTKQQAIALLEKAPGIVVKDNLNEGGYPSPLLEGNNTDKVYVGRIREDISHPLGLNLWIVADNIRNATRVNCTCVRVPVARSHLKPIYDAVGIARINVCTYQSVSGTGKEAIQELAQQTAAILNGKPVEKAVYPKQIAFNVLPHIDVFQDNGYTKEEMKMVWETQKIMGDDSILVNATTARVPVFYGHSEAVHIETRRKITAAEARKLLEKMPGVVVVDERADGGYPTAVTEAAGRDPVYVGRIREDISHPLGLNLWVVSDNVRKGAALNSAQGLFREGLVEWVSAQTYQAASGAGAKNMRELISQMGAIRDAVAPELADARSNILDIDRKVAEAMRADAFPTNEFGHALAGSLLPWIDTQLENGQSREEWKGQAETNKILGRSESPIAIDGTCVRIGAMRCHSQALTIKLNKEVAMSDVEQIIASANDWVDVVPNDRDETLKRLTPTAVTGTLGVPVGRLRKLNMGSQYLSAFTVGDQLLS"
  },
  {
    "reference": "MSNNTMVNMSIEMKYDNDDTTSPDQKLPGLEALEMVKGAASVVAEIEPSSSASSIMGSREHHERMLGPLEVCVHDLVQEQSRQHGQTIAVHSIEKDLTYAELERYADWMAKYLMVKRDIQAGHIIPFCLKKSVWTMVAMLAIMKTGAACAALDPSQPVSRIKRILDDTEAPLVIVHRDYLGLAETLDVPSIVLGPDLWEGTSSGDTDKPLPVVDATQPAYIAFTSGSTGEPKGIVVPHRSIATSMREHGPATRVDTETRALQFASYTFDMSFQEMFTTLTHGGCVCVPSEAERWNDLAGAMERLGVNWAKLTPTVVRLLHPEQVPSLRTLVVGGEPITQDIIQTWAHRVDLIVSYGPAEASIMAAVSDPLAPTALPRVIGRQVGGTLHVVDAGNHDRLVEGSGEGELLIEGPILATGYLKDQSRTDATFIIDPKWVSCDSENVTTAPRRFLKTGDLVHRDEDGVLYHLGRKDFRLKLNGRLIDLAAIETCLLGSAQIASAVVIATREDTFWQQSLVAVISAKPTPSSRPDPHDAGHIRIQTQHDLMLPLIRDLKDVAQSALPDYMVPTVWLVVDSVPRLPSGKIHRRRVVQWLENLDEFTVQEIRSMQNHTPHCEPETAMERRLRDIWAEVLNMPVSTVSTMASFTGLGGDSVAAMRLARRCAAQGLEIDVQMILQNLSITELAPRVKVAEHSTDTSADNERFSIGISLDQLCQSLPPRLRSSITLDDVVDTYRCSPMQEAILLSRSRFQGYYDVRWVAEVVSIDQDT",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein design should be able to enhance the efficiency of secondary metabolic process, nonribosomal peptide biosynthetic process.2. For general function, the protein need meet that Nonribosomal peptide synthesis (NRPS) is a key mechanism responsible for the biosynthesis of bioactive metabolites which are potentially contributing to organismal virulence3. The designed protein should contain one or more Carrier domains that are essential for its function. The designed protein sequence is ",
    "response#1": "GEPIAIVSMACRLPGGIATPEELWHLVAEGRDAIGPIPADRGWDLAEVYGPEAGSEGRATPTEGGFLDGAGDFDAAFFGISPREALAMDPQQRHLLELAWETLERAGIDPSGLRTSRTGVYAGASPSGYGEGALATAETAGHSLTGGSMAVLSGRIAYALGLEGPAVTVDTACSSSLVAVHMAVQALRAGECDLALAGGVAVMVQPIAFAEFSRQGGLAPDGRCKPFAAAADGTGWAEGVGLVLLERLSDARRNGHRVLAVLRGSAINQDGASNGLTAPNGPSQQRVIREALANAGLAAADVDVVEAHGTGTRLGDPIEAQAILAAYGAERPAERPLLLGALKSNIGHVQAAAGVAGLIKTVLAIRHGLVPATLHVDAPTPFVDWSSGAVELVAEARPWPASDRERRAAVSAFGIGGTNAHVIVEQAPAAEAAEPRSPAGLLPWTVAFTSGSTGKPG",
    "response#2": "CMEQPNAEAICSWDGKFTYNEVDVLSDSLATQLTDLGLGGSGSIIPVYMDKGRWVPIAILGVLKSGAAFTLFDPSHPLQRLKIMAEDVKAKAILCSRMTMELASQIVPQTLQIDDERGWDTIRARPGSHFSRSSRQDDALYVAFTSGSTGKPKAIVIEHGSYCTGAKEHIKAFRLSRKARVLQFALYAFDVSIMEILSTLMAGGCICILNEVQRTTPQDFEKALSSFSVTHAFLTPSFARSLRNAQLPSLDVLILGGEPMSPADAGHWASRNVVLMNAYGPAECSVNTTVQPCAAACPGNIGFTTGAACWVVDPR",
    "response#3": "CMEQPNAEAICSWDGKFTYNEVDVLSDSLATQLTDLGLGGSGSIIPVYMDKGRWVPIAILGVLKSGAAFTLFDPSHPLQRLKIMAEDVKAKAILCSRMTMELASQIVPQTLQIDDERGWDTIRARPGSHFSRSSRQDDALYVAFTSGSTGKPKAIVIEHGSYCTGAKEHIKAFRLSRKARVLQFALYAFDVSIMEILSTLMAGGCICILNEVQRTTPQDFEKALSSFSVTHAFLTPSFARSLRNAQLPSLDVLILGGEPMSPADAGHWASRNVVLMNAYGPAECSVNTTVQPCAAACPGNIGFTTGAACWVVDPS"
  },
  {
    "reference": "MSSAAKNFYQVVKSAPKGRFKGIKRDYTVEDVLKLRGSIDIDYTLATRGANKLWQLLHTEPFVPALGAQTGNQAVQMVRAGLKAIYLSGWQVAADANSAGDMYPDQSLYPANSGPELAKRINRSLRRADQIEACEAEDYLAQRDWYAPIVADAEAGFGGALNCFELMKAYIEAGAAGVHYEDQLGSEKKCGHMGGKVLIPTAQHIRHLNASRLAADVCGVPTIIVARTDAESSRLLTSDIDPRDHPYIDYEAGRTIEGFYRLKDSTAIQYCIDRAIQYAPYTDLIWMETSHPTIADAREFAEGVHKQYPDKMFAYNCSPSFNWKKHLSPSQMEKFQKELGAMGFKYQFITLAGYHANSYSMFDLARNYKEKGMLAYSGLQEGEFAAEKHGYTAVKHQREVGTGYFDAVSRAVTGGLSSTTALSGSTEEAQFQTAVASQDEEILSLTAQNVAGDEKILTPDALRFLHDLNTEFNPRRLRLLSKRNQVQADINNSLWFPDFNKETEVLRSDQGWKGAEIPRDLQDRRVEITGPTDRKMVINAMNSGANVFMADFEDSNSPTWRNQLEGQINLYDAVRNNISYTHPTTKKEYTLNEKHAVLKVRPRGWHLPEKHVLIHNQPTSGSLFDFGLFVFHNAKALIAQGSGPYFYLPKLQSAEEAQLWADVFKYTEDKLGLARGTIKCTVLIEHLLASFQLHEIIHALKDNIVGLNCGRWDYIFSYIKTFQNHRKFLLPDRFQIGMTAPFMRNYSLEVIKACHLRGIHAMGGMAAQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Ideal protein should have a binding affinity for key enzymes in the (S)-malate from isocitrate: step 2/2 pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MLIRNVGHLMTTPAVLDADGNEIPEGILDGMVTSLIALYDLKGLGRYRNSRSGSMYIVKPKMHGPDEVAFAVELFGRIEDALGMERNTLKIGVMDEERRTTVNLKACIREAKERIIFINTGFLDRTGDEMHTSMEAGPMIRKAEMKSSRWLNAYEDWNVDIGLECGLRGRAQIGKGMWTMPDLMKGMMEAKIGHPKAGANCAWVPSPTAATLHAIHYHQVNVAEVQDRLAGQRRATLDDLLTY",
    "response#2": "LLIRNVGHHMFTDAVLDQRGAEIPEGLLDAAISGLLAIHDINGVSKRRNSRSGSLYLVKPKMHGPDEVALTCELFARVEAMLGLRENTLKIGIMDEERRTTVNLNACIQTASKRVCFINTGFLDRTGDEIHTSMEAGPMIRKGEMKTQPWIKAYEDWNVDIGLIEGLPGRAQIGKGMWAAPDRMADMLAQKINHPEAGATTAWVPSPTAATLHALHYHQIDVRKRQQELKAGGRRARLSDLLATHPLTDAPAEMTDAQFRRLTLLIRNVGHHMFTDAVLDQRGAEIPEGLLDAAISGLLAIHDINGVSKRRNSRSGSLYLVKPKMHGPDEVALTCELFARVEAMLGLRENTLKIGIMDEERRTTVNLNACIQTASKRVCFINTGFLDRTGDEIHTSMEAGPMIRKGEMKTQPWIKAYEDWNVDIGLIEGLPGRAQIGKGMWAAPDRMADMLAQKINHPEAGATTAWVPSPTAATLHALHYHQIDVRKRQQELKAGGRRARLSDLLAAPPLADAPAELTDAHFRRL",
    "response#3": "MLIRNVGHLMTTPAVLDADGNEIPEGILDGMVTSLIALYDLKGLGRYRNSRSGSMYIVKPKMHGPDEVAFAVELFGRIEDALGMERNTLKIGVMDEERRTTVNLKACIREAKERIIFINTGFLDRTGDEMHTSMEAGPMIRKAEMKSSRWLNAYEDWNVDIGLECGLRGRAQIGKGMWTMPDLMKGMMEAKIGHPKAGANCAWVPSPTAATLHAIHYHQVNVAEVQDRLAGQRRATLDDLLTD"
  },
  {
    "reference": "MIAAIIAAAGSGERFGAAIPKALIQLGDRTLIEHAVSSISSVADQIIVTAPAGYEKQIQALVGDGITVVTGGATRSESVRIGLSKVLCEAEYVLIHDAARALASPALAQSVVAALKSGDVAVIPGLAQTDTVKVVDGDGIVKATPDRTSMRKIQTPQGFTYGLINQAHAKSGEATDDAALVEALGKPVRVIAGEERALKITTASDLATALQFLGQSTSFKTGVGVDAHHFEVGRPLWLAGLLWPDQDGVEGHSDGDVAAHAICDALFAASGLGDLGSNFGTDKVEYKGASGVTLLRETSALVEKAGYKISHVSVQVIGNRPKIGSRRVEAIAALSEALGGAEVALLATTTDGMGLTGEGKGIAAIASALIYSK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that can modulate the overall flux of metabolites through the Isoprenoid biosynthesis pathway, enhancing or suppressing its activity as needed.2. The protein must exhibit the following characteristics: Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds.3. A protein with metal ion binding, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity functions is required to enable terpenoid biosynthetic process.4. The protein should be able to bind a divalent metal cation ligand in a variety of conditions5. The designed protein have 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDDRYKGISSIKLLEEVGKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADALKIDVDRVNVKATTEEGLGFTGSGEGISSQAICSLLTKKRSRSS",
    "response#2": "MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDDRYKGISSIKLLEEVGKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADALKIDVDRVNVKATTEEGLGFTGSGEGISSQAICSACIRE",
    "response#3": "MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDDRYKGISSIKLLEEVGKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADALKIDVDRVNVKATTEEGLGFTGSGEGISSQAICSGQSGC"
  },
  {
    "reference": "MKVSTVERMREMDRRAMDIYGLKDELLMENAGNASFFAFFNESKGKGKNIAVVVGSGNNGGDGLVVARKFFSLGTMGYNVKIFLCSDPAKFRNSTLMNYEIVKKIGIPIEQIDNTEDFSKALKKYDVIVDAIFGTGLGRDVMGHYREIVDVINRSGKFVCSIDIPSGINGNTGQVMGTAVKANCTVTFGAPKLGNMLYPGYNHCGKLFVTRISFPPEIYDADDIHTMINMPKELPLRDESGHKGSFGDVLFIAGAKNYYGAPYFSAISFLKAGGGYSRLATPISVAPFIASKGNEIVIIPLKETGEGTISAENADMLLKLSGEVDFVIIGPGLSLNSETGKLVREFVSKVEKPVLIDGDGLTHVASGLESIKNRKYSTVLTPHPGEMARLTGLSIKDVINNRVEIVRKTAMDLNAIIVLKTAHSLVAYPDGNIFINITGNSALATAGSGDVLTGVIAAMKGLGLNTENAVSTGVFMHGLAGEFASAKLGRDGVTAQDILNHLPITVKEYRENYNNITRDYHGKLFII",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets.2. The designed protein should have a binding site that is specific to the target AMP, (6S)-NADPHX ligand.3. The designed protein must possess ADP-dependent NAD(P)H-hydrate dehydratase activity, NADHX epimerase activity, ATP binding, NADPHX epimerase activity, metal ion binding to support nicotinamide nucleotide metabolic process under certain conditions.4. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.5. The protein should be designed to have high stability and activity towards (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.6. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration The designed protein sequence is ",
    "response#1": "MMKLENAADQSGVTYDQMMENAGKGLAEHLVRIAVEMGVKEILFLCGNGNNAGDCFTAARLLTPQFKVTACLLAGVPKTRTAYAKYKMLKDIAVLEDQEAILEAVRRHRLIADGVFGIGFRGELSPFVQSVFAEIEADSEKKCIAVDIPSGGNGLSGDVAKGTPHCDMTITFGAAKTGLFLTPLSEYCGAIMLVDIGIPAPAFAHKGSFGKVLILAGSVGMTGAAYLASEATMRSGTGIVVLGIPRSLNPIMEVKLTEVMTLPLAETEKQSLGEDAKETILKLMKDFSVLGIGPGISRQLETQRLVRKIIEKSTIPLVVDADAIYALHEDTNILKKAEVPLIITPHPGEMAKLINKDIDYILDNQLDITREIAQKYGIVVVLKGARTIIANKEGEVYINVGDNSGMATGGSGDLLTGIICSLIAQGTDNFSAAITGVYIHSLAGNLARDIKGERGMIAGDVLVVGGGPYHGAPILAGLAAARMGADLIHVAMPTAALKRVKWPVDLIPEEIPDEEFLTGESVSGLVERMTTGRGVQALVIGPGSGRAEETLRAMRSLLTAAAEHGVPCVIDADAIYSLPRGEWPTGVIGVATPHGVEREGWLGEDDPAEIIAQAAESAQPSKWDLYSESAENAVIITTGVSDEIVGLGGRSSLASGGNPRMAVAGTGDLLAGSIGGLMACGMSPWAASRLACYLLREAGRVAGEQLGPGMVASDLPQGVLPAEQLETHKYKRGHLVVFSGEADKTGAARMSAVSGLKAGAGLVTIAAPGAAMAANAAHLTAVMLHAVDREADLAEWLSDKRLQTFVLGPGFGVGARARGFVTALADRRLVLDADGISSFKDDPQQLFDLFQGEPRLVLTPHEGEFSRLFPDIGDDETLGKVDKAVAAARRANSAIVYKGADTVIASPDGRALINTNAPVWLATAGSGDVLAGIIGGLLARGLPTFEAAAAAVWLHGEAGQRAGKGLTAEEL",
    "response#2": "MXSQAYDLDEMAISRFGIPSKNLMGEAGRNXSKFIKDNIKNIKSVGIVAGKGNNGGDGFAAAFYLHKLDYDITIFSINSIDCLKPDSLHYYKKCIKNNIPIIVDSEPXSKKTSFDLIIDSILGIGFNGELKENVKKWSRWINQNSLILSXDIPTGVNSDTGNISIDTVKADYTXXMGYPKIGMFLXPGKSYSGLITSVDIGFNGDSLASHFVNRSRITHKGTFGRLLNVSGSESCIGAAWMSTNAALRTGSGLVTLASVSEVTTSVATSLHECIYLPLGSKTLTSDCADKLCKNARTATAILFGCGVGNSDEAYRLLCALIDNTSCPIVIDADGINSLAPHINELKDNKGRLILTPHIKEFSRLSGLDTDYILRHKLSCAKDFAVKYGVHILLKDAYSVYASPDGFAAVNMSGNAALAKGGSGDTLAGTIGGLLAQGIETGNAVRLGAYLFGLSAQYAARERSMSGILPSELPQLYPYILNGDSLASHFVNRSRITHKGTFGRLLNVSGSESCIGAAWMSTNAALRTGSGLVTLASVSEVTTSVATSLHECIYLPLGSKTLTSDCADKLCKNARTATAILFGCGVGNSDEAYRLLCALIDNTSCPIVIDADGINSLAPHINELKDNKGRLILTPHIKEFSRLSGLDTDYILRHKLSCAKDFAVKYGVHILLKDAYSVYASPDGFAAVNMSGNAALAKGGSGDTLAGTIGGLLAQGIETGNAVRLGAYLFGLSAQYAARERSMSGILPSELPQLYPYILNTHKYRQGNLLLICGSRQYAGAALFSAYGANVSGVGMLTMIIPQSLQPLINSHIPSALVVATKETKTGVMDFTSLSDVDLSKYNTILFGMGITREKIPYLPLQEILTTSAILVIDADGFNYLTEDNLHILSQRLAPTILTPHEGEFKRLFPNINLNKNRLEGLQEAAKKINGTILLKGSKTMISHQGQYNWIIKEGTTALARGGSGDVLSGFLGGLLAQTDFIKHSIQDVVASAGWLHQQGGILAVEDLTELGVDGITL",
    "response#3": "MMKLENAADQSGVTYDQMMENAGKGLAEHLVRIAVEMGVKEILFLCGNGNNAGDCFTAARLLTPQFKVTACLLAGVPKTRTAYAKYKMLKDIAVLEDQEAILEAVRRHRLIADGVFGIGFRGELSPFVQSVFAEIEADSEKKCIAVDIPSGGNGLSGDVAKGTPHCDMTITFGAAKTGLFLTPLSEYCGAIMLVDIGIPAPAFQPDLFRDYLLPRRRNSHKGSFGNVAVIGGAPGMTGAPILAARTALKCGAGRVLVGFADNPPAYDSMQPELMCRHADDLDFSAAVLVLGPGMGMADSAQALLTRALDALSWLVLDADALNLLARQPQLQIQAAQRQHAILMTPHPLEAARLLGLSTSEIQADRVTAARKLACRFNAVVVLKGVGSIIARADGAVAVNPTGNPALATAGSGDVLSGICGALLAQGWPQWEAALAATWLHGRAADQLVEQGMGPVGLTAGELIPEVRLLLNQELLPPRPSQGHKGMFGRLMCVSGSRNMPGAAILASKAALRCGVGLFCLASDIDVCRLLIAQAPEATMLPLPTDYDGKLTQQAVPAILDYAKQATCVLIGCGLGQSEALRQAVTSLIPLLECPIILDADGLNAIAPSIDILHKAKSTVVLTPHPGEMARLLHCSVEEVQKDRMTAAKRLATRFPNTVVVLKGAGTIVATADHAHINTTGNSGMSKGGSGDILAGMIASLTAQHISPENAAIAGVYLHGLAGDCAAAENSRRAMLPSDMISQLPMLLVGGDLGFAGAIMLASQTAARSGAGLVKVMTRPEHVAALISRQPELMVSGLTSMHQLDEWLEWCDVVVLGPGLGQGGWGQQLWQKVMLATCAKIIDADALNLMATKPAFMQQLSESAKNWVLTPHPGEAARLLECSVAEVEQDRPGAVRRIQQKFGGSVLLKGAGTLIFDGNLLSVNNSGNPGMASGGMGDVLAGLIGALRAQGLSGYDAACVGTQVHGLAADQLATQFGERGLLAADLI"
  },
  {
    "reference": "MFVTGGVVSSLGKGIAAASIGALLESRGLKVTFLKLDPYLNVDPGTMSPFQHGEVYVTADGAETDLDLGHYERFGRMPMRRANNFTAGQVYEAVLRKERRGEYLGRTVQVIPHVTDEIKRCIVEGAGDADVALVEVGGTVGDIESLPFLEAIRQMGLERPHHDVLYMHLTLVPYLRASGEIKTKPTQHSVKELQGIGIRPDVLLCRTEQPLPEDERRKIALFTNVPREAVISAEDLDNIYKIPRHYHDQGLDDFVARRLDLPVGTSRLDAWDRYIHDFEHPTATVDIALVGKYVDLADSYKSLNEALDHAAAHTRTKVNVHYVDAESLQSDGTGCLAGMDAILVPGGFGERGIEGKIAAVRFAREQGLPYLGICLGLQVAVIEFARNVAGLTGAHSTEFDAATPQPVVALITEWLDDSGRREQRAAGTDMGGTMRLGGQTCLLGEDTLARRIYGKERIVERHRHRYEVNPRLLPALRTAGLAVSGRSEQDGLVEMIELPDHPWFIGCQFHPEFTSNPRDGHPLFSSFVEAARIHRAARAAQPVQA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must have at least one CTP synthase N-terminal, Glutamine amidotransferase domain.2. The designed protein should be optimized for metal ion binding, ATP binding, glutaminase activity, CTP synthase activity activity in order to improve 'de novo' CTP biosynthetic process, glutamine metabolic process.3. The designed protein should be able to compete with endogenous proteins or inhibitors for L-glutamine, CTP, ATP, UTP, Mg(2+) binding.4. The active site, Nucleophile, Nucleophile; for glutamine hydrolysis of the protein must be engineered to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).5. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen6. A protein that is capable of catalyzing reactions within the CTP from UDP: step 2/2, Pyrimidine metabolism, CTP biosynthesis via de novo pathway pathway with high efficiency and specificity. The designed protein sequence is ",
    "response#1": "KYIFVTGGVVSSLGKGIAAASIGCLLESRGLRVTLQKFDPYLNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTYAPLTQSNNVTSGRIYETIISRERRGDYLGKTVQVIPHVTDEIKANIEKVSGGVDVVLVEIGGTVGDIESLPFLEAIRQLRHKKGRENCFFVHVTLVPWIAAAGELKTKPTQHSVKELRTIGIQPDMLICRCEKPLAGDIKDKIALFCDVSADAVIEACDAKSVYEVPLGFASQNVDDIILRQFKLSCKSANLNEWKKVTIALVGKYVELPDAYKSINEAFIHAGAANECKVRVKLIAAESINAGNVDEKLKRVCGVLVAPGFGERGLEGKLVAIQYVREHNIPFFGICLGMQCAVVEFGRNVLKLKNANSYEMNPKTQHPVIHLMEAQKKVKNMGGTMRLGAYDCEVKKGTKAASIYGKAKITERHRHRYEFNNDYLAQYEAAGMVASGINPANNLVEIVELKGHPFFVAGQFHPELKSTVANPHPLFVSFIKASLNY",
    "response#2": "RYIFITGGVVSSLGKGLASAALGALLQARGYTVRLRKLDPYLNVDPGTMSPFEHGEVFVTDDGAETDLDLGHYERFTGVAARNSDSVSSGRVYSTVLEKERRGDYLGKTIQVVPHVTDEIKEFLKIGEDEVDFMLCEIGGTVGDIEGLPFFEAIRQFSHDKSRDQCIFMHLTLLPYLAASGELKTKPTQHSVKELQSIGIAPDILVCRSEHPIPDKERAKIGLFCNVRKEAVVAAYDLGSIYEAPLAYHEQGLDQAVLDAFLHDAYLSVMESLKHAGWENQSRVSISWIDSECVTPDSVDDLLAGSDGILIPGGFGDRGIEGMIVAARYARENKIPYFGICLGMQIAVIEYARDVMGLVDANSSEFAPNGLHSVIDLMPDQMGNIPKGGTMRLGEYPCAIVPETIMNRAYRGEAEIKERHRHRYEFNNVFREQLVKAGLTISGHSPDGHLVEAVELPEHVHPFYLGVQFHPEFKSRPNRAHPVFREFLAAFARVTDHVDIALVGKYVEYEDSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASFLAAAGALAAR",
    "response#3": "KYIFVTGGVLSSLGKGITAASVGTLLKHSGKKVGMLKIDPYINVDPGTMSPLEHGEVFVTKDGAETDLDIGNYERFLDDSFLKSSNFTTGQVYSSVIERERAGGYLGQTIQVVPHIVGEIVKRIKEAGEGHDILVVELGGTVGDIEGLPFMEAIRTMKHDDEVEGTFFIHVTLIPYIKAAGEHKSKPTQHSVQELRRIGITPQMIITRTEDTLPKSFKKKLAVSCDVTQDCVIEALDAATIYAVPIAFLQQNILGPIAKALDLQDAYKSIYESFIHAGAANDCRVEVVPIHSEDLEAPDADPEKIFKDIHGILVAPGFGERGIEGKLRAIRYAREKKLPFFGICLGMQCAVVEFARHVMGLKEAASTEMNAETPDPVIALMKEQKKITQKGGTMRLGAYACVLAEDSLAAKAYGATQISERHRHRYEFNNAYREQMDAAGLKATGINPESGLVEIVELRDHPWFVGVQFHPELKSTVENPHPLFVAFVESIVDKG"
  },
  {
    "reference": "MGGCAGSRRRLLDSEGEETAPEPRPRLLDRQGALWKNAMGFWLLGLCNNFSYVVMLSAAHDILSHQRASGNQSHVDPDPAPTTHNSSSRFDCNSVSTAAVLLADILPTLVIKLLAPLGLHLLPYSPRVLVSGICSAGSFILVAFSHSVGTSLCGVVLASISSGLGEVTFLSLTAFYPRAVISWWSSGTGGAGLLGALSYLGLTQAGLSPQHTLLSMLGIPALLLASYFFLLTSPEPQDPGGEEEAETSARQPLINSEAPEAKPDSSSNLSLQERWTVFKGLLWYIVPLVLVYFAEYFINQGLFELLFFRNTSLTHAQQYRWPLLLSEDEEDTKRVVRSAKDKRFEELTNLIRTIRNAMKIRDVTKCLEEFELLGKAYGKAKSIVDKEGVPRFYIRILADLEDYLNELWEDKEGKKKMNKNNAKALSTLRQKIRKYNRDFESHITNYKQNPEQSADEDAEKNEEDSEASSDEDEDEDGVSAAAFLKKKPEAPSGDSRKFLKKMESASSSSSCRFSCFSKDEDEDSEDSEDDEDWDTGSTSSDSDSEEEEGKQTVLASRFLKKAPTTEEDKKAAEKKREDKAKKKHDRKSKRLDEEEEDNDGGEWERVRGGVPLVKEKPKMFAKGTEITHAVVIKKLNEILQARGKKGTDRAAQIELLQLLVQIASENNLGEGVIVKIKFNIIASLYDYNPNLATYMKPEMWQKCLDCINELMDILFANPNIFVGENILEESENLHNVDQPLRVRGCILTLVERMDEEFTKIMQNTDPHS",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein have PCI domains with specific sequence motifs that are crucial for its activity.2. The protein can be expressed in eukaryotic translation initiation factor 3 complex, vacuole, membrane, eukaryotic 43S preinitiation complex and perform RNA binding, translation initiation factor activity without triggering undesirable cellular responses.3. The protein must exhibit the following characteristics: The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation; The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.; The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition; The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF- 2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC).4. The protein should contain a Helical membrane-spanning region.5. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "MQRMSTRVLLATLSIPITPERTDIARLLDMDGIILEKQRRLATLLGLQAPPTRVGLINDMVRFNMLQYVVPEVKELYNWLEMDFHPLKLCTRVTKVLDWVKEQAEKEPELQQYVPQLQSNTILRLLQQVAQLYQTIEFSRLASLVPFVDAFLLERAIVDAARHCDLQVRIDHSSRTLSFGSDLELDNLDGEKSCSSLSHEQNMEKMRLLTFMGMAVDNKEISFDTIQQELQIGADEVEAFIIDAVKTKMVYCKIDQTQKRVVVSHSTHRTFGKQQWQQLYDTLNTPDAIRMFSHILVYVSRTKNFQKGGNSYDAIAKKNDQMYALIAICVALHPTRLDDTIHSALREKYGEQLLRLQHGGPDALPLFEELFRSACPKFISPTPPDFDNPALNIDPVDHHTAIFMDEVKNTLYNPTIRSYLKLYTTMDLKKLAGFLEVQPEVLRSWLLVNKQRSRQVRWVEGGLLEGEVVSANDLDYALENDIDAIRMFSHILVYVSRTKNFQKGGNSYDAIAKKNDQMYALIAICVALHPTRLDDTIHSALREKYGEQLLRLQHGGPDALPLFEELFRSACPKFISPTPPDFDNPALNIDPVDHHTAIFMDEVKNTLYNPTIRSYLKLYTTMDLKKLAGFLEVQPEVLRSWLLVNKQRSRQVRWVEGGLLEGEVVSANDLDYALENDL",
    "response#2": "MQRMSTRVLLATLSIPITPERTDIARLLDMDGIILEKQRRLATLLGLQAPPTRVGLINDMVRFNMLQYVVPEVKELYNWLEMDFHPLKLCTRVTKVLDWVKEQAEKEPELQQYVPQLQSNTILRLLQQVAQLYQTIEFSRLASLVPFVDAFLLERAIVDAARHCDLQVRIDHSSRTLSFGSDLLDNYSFLNDAASSDEESEEEDKIIVLSAKDKRFAEMEAAIHSIQNAVKNTDWVLASSELDKLFRFIARHLVTIVASPIPAEGHIPPRFLETLVSLEADVAKTLAAEKSAPKKMAPAKAKAVNGIRQTLKKKSKEFDGVLKTYNEDPEAYTAAYEKANAAPAAPKPKKAAVTTPGEGEDDEFMTIGKGGKTLNLTADGVFKTLREIFEARGRKNTDRAETIKILTKLLEVSETTYQKIRVLLALVPARLDYSQHLVSIPQESWVTALQEFDQLISILIAEPDYLVQETVGEYDDLVERTPEVVDGKKQKVLIAGSLISLLESLDSELTKTLQHTDAHEKGDEYIDRLKHEAPLYTTIVKAQTLFEREQLSDNIARTVIRRLEHIYAKPDIIIEHFESKATQAAAGLKSEITPFDVKRDASGVIHDLAVFIYQSDAPVLRARAILYHIFNQALHGRYHQARDLLLMSHLQDTISHADVTTQILYNRAVMQVGLAAFRLGFIPECQTILSEMFSTLRQKELLAQSVQRYNIQLSPEQELLEKRRLLPFHMHLNVELLEAAYLTSCMLVEIPLLASVEDTERIGVDLIMKTCFSPNRVIGLSSDLQQVGGASARIQDALSTVLQYAEDVLSGKVAADNTVGRFLMSLVNQVPKIVPEDFETMLNSNINDLLMVTYLANLTQSQIALNEK",
    "response#3": "MQRMSTRVLLATLSIPITPERTDIARLLDMDGIILEKQRRLATLLGLQAPPTRVGLINDMVRFNMLQYVVPEVKELYNWLEMDFHPLKLCTRVTKVLDWVKEQAEKEPELQQYVPQLQSNTILRLLQQVAQLYQTIEFSRLASLVPFVDAFLLERAIVDAARHCDLQVRIDHSSRTLSFGSDLFLNDAASSDEESEEEDKIIVLSAKDKRFAEMEAAIHSIQNAVKNTDWVLASSELDKLFRFIARHLVTIVASPIPAEGHIPPRFLETLVSLEADVAKTLAAEKSAPKKMAPAKAKAVNGIRQTLKKKSKEFDGVLKTYNEDPEAYTAAYEKANAAPAAPKPKKAAVTTPGEGEDDEFMTIGKGGKTLNLTADGVFKTLREIFEARGRKNTDRAETIKILTKLLEVSETTYQKIRVLLALVPARLDYSQHLVSIPQESWVTALQEFDQLISILIAEPDYLVQETVGEYDDLVERTPEVVDGKKQKVLIAGSLISLLESLDSELTKTLQHTDAHEKGDEYIDRLKHEAPLYTTIVKAQTLFEREQLSDNIARTVIRRLEHIYAKPDIIIEHFESKATQAAAGLKSEITPFDVKRDASGVIHDLAVFIYQSDAPVLRARAILYHIFNQALHGRYHQARDLLLMSHLQDTISHADVTTQILYNRAVMQVGLAAFRLGFIPECQTILSEMFSTLRQKELLAQSVQRYNIQLSPEQELLEKRRLLPFHMHLNVELLEAAYLTSCMLVEIPLLASVSYI"
  },
  {
    "reference": "MRTHYCNGIHEGLIGQTVTLCGWAQRRRDHGGVIFIDLRDREGLVQIVADPDQVDAFAAANECRSEFVLQVEGVLRARPAGTENPHMPSGKVELAAARIRILNRSEPVPFPLDEEDIAENLRLKYRYLDLRRPEMLHRLRLRHQVTRFVRGYLDNAGFIDVETPVLTRSTPEGARDYLVPSRTQPGHFFALPQSPQLFKQLLMVAGLDRYYQITKCFRDEDLRADRQPEFTQIDIEASFVDEEAVMGTAEPMIRGLFAEVLGVQLPDPFPRMTYRDAMHRFGVDRPDLRNPLELTELTDLMRAVDFKVFREAAERPHGRVACLRVPGGAQLSRAQIDAYTQFTAIYGARGLAWIKVNALDQGNEGLQSPIVKFLPEMVLSEILRRSGAAAGDILFFGADTAKIVNEALGNLRNRVAADLGLLEGEWCPVWITDFPMFDYDDKEDRWTSTHHPFTAPQTEHLETLGQDPGNALARAYDLVLNGNEIGGGSIRIHQEAVQEKVFAALGIGAEEARDKFGFLLDALHYGAPPHGGLAFGLDRLVMLLCGAETIRDVIAFPKTQKAGCLLTEAPGTVADKQLQELGIRLRPGVGGGVPNP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have aspartate-tRNA ligase activity, nucleic acid binding, aspartate-tRNA(Asn) ligase activity to facilitate aspartyl-tRNA aminoacylation.2. For general function, the protein need meet that Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).3. The designed protein should have a binding site that is specific to the target ATP ligand. The designed protein sequence is ",
    "response#1": "KEELRLKYRYLDIRRKVIGENLRFRHKAVIAIRRYLDNLGFIEVETPILTKSTPEGARDYLVPSRIHPGTFYALPQSPQIFKQLLMVGGMDRYYQIARCFRDEDLRADRQPEFTQVDMEMSFVTQEDILVHLENMFKYIMKECIGHIISEPFIRMTYKEAMDRYGTDKPDLRFDLSIKDITDIARDTDFEIFKKTETVRAINYKNGASMTRSEIEELTEEAESCGAKGMAWIAIQPDGSLYSILTKYISKEDIDEIIKITDARAGDFILFCADTIKNARRILGQLRIFIAKKLGIIDANMFCFAIITDFPLFEYSEEDKRYVAAHHPFTSPNPEDIMHMLTDKECVRALSYDIVLNGTELGSGSIRIHDSKTQNLMFEALGLSNEQIQEKFGFFIKAFSYGTPPHGGFAFGLDRLIMILLKASSLRDVIAFPKTS",
    "response#2": "LRLRSAANKAARTVLDSHDFTEIETPTLTRSTPEGARDFLVPARLRPGTFYALPQSPQLFKQLLQVAGMERYYQIARCYRDEDFRADRQPEFTQLDVEMSFVDQDDVIALGEEIISEVWKLIGYEIKTPIPRMTYADAMRRYGSDKPDLRFDIEITECTEFFQDTTFRVFKNEYVGAVVMTGGASQPRRQLDAWQEWAKQRGAKGLAYILVGEDGELSGPVAKNITDAERAGIAAHVGAQPGDCIFFAAGDTKSSRALLGAARGEIAKKLDLIKEGDWAFTWIVDAPMFEPAADATASGDVALGNSKWTAVHHAFTSPKPEFLDNFDTNPGDALAYAYDIVCNGNEIGGGSIRIHQRDVQERVFEVMGITGEEAREKFGFLLDAFAFGAPPHGGIAFGWDRIVSLLGGFDSIRDVIAFPKSKGACPLTGAPSYVA",
    "response#3": "VAKTLLNNLKPEDYEKIEKPSALTEMFAELKSGNITVILLQDAINAYLNDAQFDYQAYFKENSISDSSIDDSVQKVIEENGDIAEEIKAGNQGKAGILVGKVLQIIGKGASGKVIREKILDAL"
  },
  {
    "reference": "MAAEVKKGIDTTKNPIPFEMAQIPGPEMAKTYLPKVIGAIIRKAKRPLLVVGAELFDDPVMFDKMIEMGKMGIPIAATAHSVKGFVDRGYLENVYQIGLHPLTNFLRFPDWKGLDGQGQYDVVIFLGIYYKFANGMLSTLKNFNRDIKRVSIDRYYHVNADMTFGNLAFNPDDYHAAVDEVIAAMKK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2); The precise role of the epsilon subunit is unclear; it may have a stabilizing role within the alpha(2)epsilon(2) component and/or be involved in electron transfer to FAD during a potential FAD-mediated CO oxidation.; The alpha-epsilon subcomponent functions as a carbon monoxide dehydrogenase The designed protein sequence is ",
    "response#1": "FKARDARTKDLHDEDVDVFYGCTLCQSFAPTNVCVVSPDRVSLCGAINWFDGRAAAKVDPEGPQFAIEKGELLDAKTGEYSGVNEVAKKLSSGEFDKIKLHSFFDAPHTSCGCFEDVGFYIPEVDGIGWVNREYQGMAPNGLGFSTMAGQTGGGKQIVGFLGIGINYFYSPKFIQADGGWNRVVWLPSMLKEKIDEAIPDDMKDKIATEKDVTDIESLKTFLKEKNHPVL",
    "response#2": "FKARDARTKDLHDEDVDVFYGCTLCQSFAPTNVCVVSPDRVSLCGAINWFDGRAAAKVDPEGPQFAIEKGELLDAKTGEYSGVNEVAKKLSSGEFDKIKLHSFFDAPHTSCGCFEDVGFYIPEVDGIGWVNREYQGMAPNGLGFSTMAGQTGGGKQIVGFLGIGINYFYSPKFIQADGGWNRVVWLPSMLKEKIDEAIPDDMKDKIATEKDVTDIESLKTFLKEKNHPVC",
    "response#3": "FKARDARTKDLHDEDVDVFYGCTLCQSFAPTNVCVVSPDRVSLCGAINWFDGRAAAKVDPEGPQFAIEKGELLDAKTGEYSGVNEVAKKLSSGEFDKIKLHSFFDAPHTSCGCFEDVGFYIPEVDGIGWVNREYQGMAPNGLGFSTMAGQTGGGKQIVGFLGIGINYFYSPKFIQADGGWNRVVWLPSMLKEKIDEAIPDDMKDKIATEKDVTDIESLKTFLKEKNHPVC"
  },
  {
    "reference": "MFNLMKTAVLMAAITALFMAIGSVLGGQQGMAIALVVALGMNFFSYWFSDKMVLKMYNAQEVDASSAPQFYGMVRELAAKAELPMPKVYLINEDAPNAFATGRNPQNAAVAATTGILRVLSERELRGVMAHELAHVKHRDILISTISATMAGAISMLANFAMFFGGRGSDGRPANPIAGILVMLLAPLAASLIQMAISRAREFEADRGGAEISGDPQALASALQKIQRYAQGIPLEAAERHPETAQMMIMNPLSGGGLRGLFSTHPATEERVAKLMAMVPQRV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. The protein design should incorporate a binding pocket that specifically recognizes Zn(2+) to enhance enzymatic efficiency.3. The designed protein should be involved in proteolysis by localizing to plasma membrane and exhibiting zinc ion binding. The designed protein sequence is ",
    "response#1": "DHPEIWHIVEDMALVANIPMPKVYIVNDASPNAFATGNSPKNAAVAVTTGILERLNREELEGVIGHEVSHIRNYDIRLSTIALALSSAIAMLVNIGMRSFWWGGGRRRNDNDNEGGSLEIVMMIISIVLVILGPIATTIAQLALSRNREYLADASSVELTRNPLGLIHALQKISVSEPMKEADPSSAALYISDPFKKKRTMAHLFDTHPPIEDRIQRLEGR",
    "response#2": "VPRGVLLIGSPGCGKTLLARAIAGEADVPFFSISGSDFVEMFVGVGASRVRDLFKQAKDSSPCIIFLDEIDAVGRKRGSGMSSGGHDEREQTLNAILVEMDGFESSDQVIVIAATNRADVLDPALTRPGRFDRQIHVPLPTLEER",
    "response#3": "QNEVWLVDTVSQLSKKAGLAMPEVAIYDGAPNAFATGPSKSRSLVAVSTGLLQSMDRKQVEAVLAHEVAHIDNGDMVTLTLIQGVLNTFVIFLSRVIAYAIDSFLRSDDDESGSPGIGYWISSIIFEIMFGILASVVVMYFSRKREYRADAGAAVLLGDRRPMIDALRALGGLQAGQLPKEMAASGIAGGGMMALFSSHPPLESRIAALEA"
  },
  {
    "reference": "MSKNKLSKGQQRRVSANHDRRLKQRADKPEPDDNLFGEVRDGVAISRFGMHADVEDADGSVHRCNIRRTIRSLVTGDRVRWRPGVEGGATVKGIVEAVHERTSVLTRPDFYDGVKPIAANINQIVIVSAILPELSLNIIDRYLVASETLNVEPLLVLNKTDLLDDEGREFVDEQMEIYRHIGYRVLMVSSHKKEGLRELEEALIGRVSIFAGQSGVGKSSLLNALLGLELGDEEILTKDVSDVSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLWHLEPEQITRGFVEFRDYLGGCKFRDCKHDTDPGCAIREAVDKGEIDASRFVNYHHILESMSEVQAKTRRSFSADKE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "MSEAEKKASQDAQHKEPMADSETQLDSDSAPSSQAEKPAKTKYPLSFWLAFGALCLTGLISAMEGSIVSTSLPSIIAELKAAFQPLYGQLADLWGRRYVMILATAIFLLGSGICGGANSMDMLIWGRAVQGIGAGGINMLVDLIICDLVPMRERGNFIGLLFLFVSIGTTSGPIIGGALTDNTTWRWVFYINLPMGGAALVLLVLFLQVKWKKELSTRDRLKRIDVVGNAILVGATFSILYALTYGGTRYPWSAANIVAPFVLGFVGLGIFIAWESNKRWCPYPVMPLHHFNSRTASASFFISFMTMILAFWVVYFYPVYFQSVLGNTPTISGVHLLPFEVSFPIFAAVGGGLVSKTGRYKPIHMVATSIVTIAIGASSVLTQHTHKAAWAVLQIFIGMGLGSLISTTLQAVQAGLPESETAASTATWAYMRSLGTIWGVSVPAAIFNNRFDQLSGQLDPSIRDNFVRGQAYEHATAQFVQSFEPATREVVIGAYTDALRRVWLVSIAFGAVTVLSTLFEKELTLRTQ",
    "response#2": "MSEAEKKASQDAQHKEPMADSETQLDSDSAPSSQAEKPAKTKYPLSFWLAFGALCLTGLISAMEGSIVSTSLPSIIAELKAAFQPLYGQLADLWGRRYVMILATAIFLLGSGICGGANSMDMLIWGRAVQGIGAGGINMLVDLIICDLVPMRERGNFIGLLFLFVSIGTTSGPIIGGALTDNTTWRWVFYINLPMGGAALVLLVLFLQVKWKKELSTRDRLKRIDVVGNAILVGATFSILYALTYGGTRYPWSAANIVAPFVLGFVGLGIFIAWESNKRWCPYPVMPLHHFNSRTASASFFISFMTMILAFWVVYFYPVYFQSVLGNTPTISGVHLLPFEVSFPIFAAVGGGLVSKTGRYKPIHMVATSIVTIAIGASSVLTQHTHKAAWAVLQIFIGMGLGSLISTTLQAVQAGLPESETAASTATWAYMRSLGTIWGVSVPAAIFNNRFDQLSGQLDPSIRDNFVRGQAYEHATAQFVQSFEPATREVVIGAYTDALRRVWLVSIAFGAVTVLSTLFEKELTLRTES",
    "response#3": "MSEAEKKASQDAQHKEPMADSETQLDSDSAPSSQAEKPAKTKYPLSFWLAFGALCLTGLISAMEGSIVSTSLPSIIAELKAAFQPLYGQLADLWGRRYVMILATAIFLLGSGICGGANSMDMLIWGRAVQGIGAGGINMLVDLIICDLVPMRERGNFIGLLFLFVSIGTTSGPIIGGALTDNTTWRWVFYINLPMGGAALVLLVLFLQVKWKKELSTRDRLKRIDVVGNAILVGATFSILYALTYGGTRYPWSAANIVAPFVLGFVGLGIFIAWESNKRWCPYPVMPLHHFNSRTASASFFISFMTMILAFWVVYFYPVYFQSVLGNTPTISGVHLLPFEVSFPIFAAVGGGLVSKTGRYKPIHMVATSIVTIAIGASSVLTQHTHKAAWAVLQIFIGMGLGSLISTTLQAVQAGLPESETAASTATWAYMRSLGTIWGVSVPAAIFNNRFDQLSGQLDPSIRDNFVRGQAYEHATAQFVQSFEPATREVVIGAYTDALRRVWLVSIAFGAVTVLSTLFEKELTLRTR"
  },
  {
    "reference": "MTRYVFITGGVVSSLGKGLSSAALGALLQARGFKVRLRKLDPYLNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTGVSARNSDNVTTGRIYSTVITRERRGDYLGGTVQVIPHVTDAIKQFVTSDITDEDFVLCEIGGTVGDIEGLPFLEAIRQLRNELGREKTMYVHVTLVPFLGAAGELKTKPTQHSVKELLSVGIQPDLLLCRCEREIAGDARRKLALFCNVQESRIIPAEDVESIYEVPITYHEQGFDIEVLNHFGLIEERDREVDLSRWRGIVHSILNPEGEVNIAIVGKYTNLMDAYKSLSEALTHGGIANNVRVNTRWIEAEIFEQENHIHKLENCHGILVPGGFGERGAEGKIQAAEFARRRKVPYFGICFGMQMAVIEAARNLAGIPEANSTEFGPCKDPVVGLLTEWTRGNTVEQRDKDAELGGTMRLGSYPCRVEPGSRIAEIFGGELEINERHRHRYEVNVNYKQRLENAGLKFSGLSPDNRLPEIIELQDHPWYIGVQFHPELKSRPFAPHPIFKSFIKAAVDQARLV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that is capable of catalyzing reactions within the CTP from UDP: step 2/2, Pyrimidine metabolism pathway with high efficiency and specificity.2. The presence of Glutamine amidotransferase domains is critical for the interaction of the designed protein with its intended targets.3. The designed protein is the optimization of the Nucleophile, Nucleophile; for glutamine hydrolysis to achieve high catalytic efficiency for H2O + L-glutamine = L-glutamate + NH4(+).4. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen5. The Mg(2+), UTP, L-glutamine binding site should be located in a region of the protein that is accessible to the ligand.6. The designed protein should be optimized for glutaminase activity, ATP binding, metal ion binding, CTP synthase activity activity in order to improve 'de novo' CTP biosynthetic process, glutamine metabolic process. The designed protein sequence is ",
    "response#1": "RFIFVTGGVVSSLGKGIASASLGAILEARGLKVAMIKLDPYINVDPGTMSPFQHGEVFVTEDGTETDLDLGHYERFVHTTTGRHSNYTTGRIYETVIQKERRGDYLGGTVQVIPHITDEIKRSIHAGAGDADICLVEIGGTVGDIESLPFLEAIRQMGTELGSGHALFIHLTLVPFISASGEIKTKPTQHSVKELRSIGIQPDILICRGDRPLPPEERRKIALFTNVSERAVISAVDVEDIYQIPELLHRQALDQIVSERFGLDVPEPDLAEWEQVVYQQQNPAHEVKIGLVGKYVELADAYKSIIEALIHAATYNDRKAKIELIHSESLNNENVAEKLKEMDGILVAPGFGHRGIDGKLVAVKYARENNIPFLGICLGMQCAVIEFARDVLKYDNANSREMDHSTPYPVIDLMEDQKNVTDMGGTMRLGAYDCTLTKNTNSHKAYASEHTKERHRHRYEFNNEYLKAFEAAGMKPAGCNQETGLVEIMEIPSHKWFVGVQFHPEYSSTVLKPHPIFMAFVKAALDHKAGR",
    "response#2": "KHIFVTGGVVSSLGKGLTSASIGLLLERRGLNVRMQKLDPYINVDPGTMSPYQHGEVYVLDDGSETDLDLGHYERFTNSPLSKKSNYTTGRIYRSVIEKERRGEYLGGTVQVVPHVTDEIKAAVLDLGEPGVDVVITELGGTVGDIEGLPFLEAIRQVALDVGRQNCLYIHLTLVPYLKAAREAKTKPTQHSVGQLRQIGIQPDILIVRTERPLGRENAEKIALFCNVEKNAVIEEMDKEFSIYEVPLGLAEHKLDRLITDRLGLEAGPADLSAWEEVVDRLRRPAHTVTIGLVGKYIDLPDAYLSVTEALKAGGFAHHAKVSIRWVASDECETPEGAARNLADLDGMLIPGGFGVRGIQGKLGALKFARENGIPTLGLCLGLQCMVIEYARDIVGLAGASSSEFDPDTEFPVIATMAEQIAIIEGGDLGGTMRLGLYEAALKPGSIVASLYGSEHAAERHRHRYEVNNAYRQQIADAGLVFSGISPDRTLVEFVELPRDVHPFYVGTQAHPELRSRPNHAHPLFRGLVAAALDHQRGRPLPLQDAYKSILEALIHAGAMNECKVEVRNIHSEYLTKENAKEKLQNLDAVLVAPGFGSRGIEGKIEAIRYARVNKVPFFGICLGMQMACVEFARNVLGMPKAHSTEMDPDTKDGVISMMESQKTITMMGGTMRLGSYACELRPDSTTAEIYGTANITERHRHRYEFNNEYLAAFEEAGFIPVGKNPLTDLVEVVEMKDHPFYIGVQFHPEYKSTVENPHPLFVGFVVRAAASG",
    "response#3": "KHIFVTGGVVSSLGKGLTSASIGLLLERRGLNVRMQKLDPYINVDPGTMSPYQHGEVYVLDDGSETDLDLGHYERFTNSPLSKKSNYTTGRIYRSVIEKERRGEYLGGTVQVVPHVTDEIKAAVLDLGEPGVDVVITELGGTVGDIEGLPFLEAIRQVALDVGRQNCLYIHLTLVPYLKAAREAKTKPTQHSVGQLRQIGIQPDILIVRTERPLGRENAEKIALFCNVEKNAVIEEMDKEFSIYEVPLGLAEHKLDRLITDRLGLSSGAPDLTEWNDLVDRLKNPQHTDSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASFVVTIAIVGKYTGLTDAYKSLVEALIHGGLANTVRVRFDWIEGEAFEKEEALIGERLKGVHGVLVPGAFGERGSEGMIRAVQFAREHAIPYFGVCFGMQMAMIEAARNLAGIKQASSTEFGPTSEPIVGLMTEWTRGNEREVRAADGELGGTMRCGAYEAVLERGSRVAEIYGATSIWERHRHRYEVNIAYREAIEKTGLHFSGLSPDGVLPELCERADHPWFVGVQYHPELKSRPFEPHPLFASFVAAANLAGKAIDSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASFV"
  },
  {
    "reference": "MADESNKVAAEVRDSFGKGAARKIRAAGKIPAVIYGHGSEPVHITLPAHQVGLLLRKANAVLDIDIAGKTQLALVKDVQKDPVLQIIEHLDLLVVRRGEKVQVEVPVHVEGEPFSGTIAMLDIPTIKLEVEATNIPERIVIDVTGAEEGTQYHAKDFALPAGAVLAEDEDLLLLNIIVPAAARAEDEEAVVEAAGDQAAE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. For general function, the protein need meet that This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance.2. The designed protein should be involved in translation by localizing to ribonucleoprotein complex, ribosome and exhibiting 5S rRNA binding, structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "MYRAMRREGRIPAVLYGHRGTQSLALELEQRTAEDLLKRVTINNTILPLKVERGWSGDVLLREVQHDAVGGKLLHLSFFK",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#3": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTRL"
  },
  {
    "reference": "MTTTGKVNLLDLDRSGLEAFFVTLGEKPFRATQVMKWIYHERVIDFAAMSNLSKALRERLADCAEIRLPEVALDQASRDGSHKWLIRLDSGNCIETVFIPEPDRGTLCVSSQIGCPLDCSFCATAQQGFNRNLTVAEIIGQVWHASRLLGERRNGDRIITNIVLMGMGEPLLNFASVVKATDLMQDDLAFGISKRRVTLSTAGVVPALYRLRDVSEVSLAVSLHAPTDELRNELVPLNRKYPLAELLAACKHYIADKPHRRITWEYVLLAGVNDSEAHARALAALIRDIPSKVNLIPFNPFPNTRYQRSDPATIARFHAVLLAHDLTVVTRKTRGDDIAAACGQLAGQVQARGRRLERPLPLAVG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for [4Fe-4S] cluster, S-adenosyl-L-methionine binding.2. The designed protein must have at least one Radical SAM core domain.3. A protein that can perform 4 iron, 4 sulfur cluster binding, metal ion binding, tRNA binding, rRNA binding, tRNA (adenine-C2-)-methyltransferase activity in cytoplasm to support rRNA base methylation.4. The design of the protein must focus on the precise arrangement of amino acids in the S-methylcysteine intermediate, Proton acceptor to enable effective catalysis of adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.5. The presence of [4Fe-4S] cluster is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "TKRRKTICVSTQVGCSLRCSFCMTGKTGFTRDLSVWEIADQVRRVESIVEKRISNVVLMGIGEPLLNLKNVMKAIKILNCENGMNIGARKITISTAGIIPGIIALSQESIQVKLAVSLNAATDRKREILMPINRKYNLGRLFEALEDYYSLKGKRITFEYVLIKGFNDSISDAKRLASITENIPCKINIIPFNSIGPGKFKRPDPERIKKFINWLSPIAQAVSLRVPYNETVDLSAYTLCVSTQVGCAVGCTFCFTTKDGLIRNLTTSEIVDQYIQSQRFVGVEKRISNVVFMGMGEPLANYENLKKAIQIMTDDRMLGLSNRKITVSSSGIIHQIKKMYQDKSFPQVRLAVSLNAADQETRKKIMPISETNSIEDLMKTLNSLPVKTGYRIMLEYVLIKGVNDRPEDAHRLARLIGKNKKRYKVNLIPFNPYPGSPFERPEEERVNRFHQILWQYNE",
    "response#2": "TKRRKTICVSTQVGCSLRCSFCMTGKTGFTRDLSVWEIADQVRRVESIVEKRISNVVLMGIGEPLLNLKNVMKAIKILNCENGMNIGARKITISTAGIIPGIIALSQESIQVKLAVSLNAATDRKREILMPINRKYNLGRLFEALEDYYSLKGKRITFEYVLIKGFNDSISDAKRLASITENIPCKINIIPFNSIGPGKFKRPDPERIKKFINWLSPIAQAVSLRVPRQGWRMETR",
    "response#3": "EEDRGTLCVSSQVGCTLTCTFCHTGTQRLVRNLTPGEIVGQILIARDRIGEWPGATRPTDGPLPNAERLITNIVLMGMGEPLFNFDNVRQAMEIAADGDGISVSKRRITLSTSGIAPMIPRCGVELGVMLAISLHAVRDDLRDVLVPINRKYPIAELLDACRAYPSLSNAKRITFEYVMLRGVNDSLADAKALVRLLAGIPAKINLIPFNPWPGAPYESSEWEQIEKFAEIINKAGYASPVRTPRGRDILAACGQLKSETEKLKARARAEAAAAE"
  },
  {
    "reference": "MVHFTAEEKAIITSLWAKVNVEETGGEALGRLLVVYPWTQRFFDNFGNLSSASAIMGNPKVKAHGKKVLSSLGEAVTHMDDLKDAFAHLSELHCDRLHVDPENFRLLGNVLVIVLAHHFGREFTPQVQAAWKKLMSAVAIALGHKYH",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein should have a high affinity for heme b.2. The ideal protein would be one that plays a critical role in positive regulation of cell death, carbon dioxide transport through oxygen binding, oxygen carrier activity, metal ion binding, heme binding within hemoglobin complex.3. The protein must exhibit the following characteristics: Gamma chains make up the fetal hemoglobin F, in combination with alpha chains.. The designed protein sequence is ",
    "response#1": "AHIVETGLGAYHALFTAHPQYIIHFSRLEGHTIENVMQSEGIKHYARTLTEAIVHMLKEISNDAEVKKIAAQYGKDHTSRKVTKDEFMSGEPIFTKYFQNLVKDAEGKAAVEKFLKHVFPMF",
    "response#2": "AHIVETGLGAYHALFTAHPQYIIHFSRLEGHTIENVMQSEGIKHYARTLTEAIVHMLKEISNDAEVKKIAAQYGKDHTSRKVTKDEFMSGEPIFTKYFQNLVKDAEGKAAVEKFLKHVFPML",
    "response#3": "AHIVETGLGAYHALFTAHPQYIIHFSRLEGHTIENVMQSEGIKHYARTLTEAIVHMLKEISNDAEVKKIAAQYGKDHTSRKVTKDEFMSGEPIFTKYFQNLVKDAEGKAAVEKFLKHVFPMF"
  },
  {
    "reference": "MDKKKVLRIAAIVAAVLIAIYTFSVLTDKTRGYEEVPTSVAMQQLNDKNVDKVQINDREQQIQATLKNPIEVDGKEGIEQIIASYPNRASGDIFNAVKASDATDFDTKVTHDSILGSLLVSLVPMLLIFGLLMFLLSRMQGGGGMNAFGMGKSRHKELNKDNPENTFDDVAGADEAVEELDEIRDFLSDPSRYEKLGAKIPRGVLLYGPPGTGKTLLARAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFKQAKENSPCIIFVDEIDAVGRQRGSGMGGGHDEREQTLNQLLVEMDGFGDREGVILMAATNRPDILDPALLRPGRFDRQIPVTNPDLTGREQILRVHAKGKPLADDVDLKSLAKRTAGMSGADLENVLNEAALLTARVDGNVITGDALEEATDRVIGGPRRSTKIISETEKKITAYHEGGHTLAAWALKDIDRVYKVTILARGRTGGHAMTAPEDDKGMYNRSELFARLVFAMGGRAAEELVFGNPTTGASADIEMATNTARSMVMELGMSPKIGAVKYGIDNGDPFRSQGSSGSESKPSPEVAAVIDNEVRMLMDKAQTIAYEILRDNREYLDKLAEKLLEKETLRRPDLEAIFDGIEIREITDIFPMQDVARPKDFREPVKTPNELAKERGEEPPKRNDFFSAARKARIERRKAAQEKQLREAREKDKQHVGAHAGAQNTLPDKPGHDHLWGGRDYGTGSPNKSGTPSRGGAPGSDAPHHNVSPNGGIKQEEQRGFRLPDHEQPDHPWTTDDA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.2. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. The protein should be able to bind ATP ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "YMAQRVVGTGSFGVVFQAKCLETGEQVAIKKVLQDKRYKNRELQIMRLQDHPNVVRLRHSFFSTTDKDELYLNLVLEFVPETVYRALKHYTKMNQHMPIILVQLYTYQICRALNYLHRVVGVCHRDIKPQNLLVNTHTHQLKICDFGSAKMLVPGEPNISYICSRYYRAPELIFGATEYTNAIDMWSGGCVMAELLLGQPLFPGESGIDQLVEIIKILGTPTREEIRCMNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALEACAHPFIDNRLRKSQEKRRTTQPVAKSAGCIFKNPDSIPAGKLVDELRLKNSRVGKARVSEVHGNFIVNDGGATAAEMLELIELIKTRARMNTGTL",
    "response#2": "LKDAYKSLIEAIAHGGLANKVKVNIEWIDSQLFEKENSVLALQKAHGILVPGAFGARGAEGKIRAIQFARERKIPFLGICFGMQLACIEAARNIAQIENASSSEFCETENPIVGLMTEWLKGDVLEKRTRNGDLGGSMRLGAFAAELKEESHIAKIYGMTKIVERHRHRYEVNIDYKDKLEQCGLIFSGMSPDGVLPETIEYVDHPWFIGVQYHPELKSRPFDPHPLFSSFIS",
    "response#3": "YMAQRVVGTGSFGVVFQAKCLETGEQVAIKKVLQDKRYKNRELQIMRLQDHPNVVRLRHSFFSTTDKDELYLNLVLEFVPETVYRALKHYTKMNQHMPIILVQLYTYQICRALNYLHRVVGVCHRDIKPQNLLVNTHTHQLKICDFGSAKMLVPGEPNISYICSRYYRAPELIFGATEYTNAIDMWSGGCVMAELLLGQPLFPGESGIDQLVEIIKILGTPTREEIRCMNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALEACAHPFYRLRNMNE"
  },
  {
    "reference": "FFQLAVFVVILFNINLLSASAGSKGSSAPQSSGDSVVAEFCDTNCTMKKDGKWTECNGDCFCVHVGNETVGRCMRLDGDYDYTSSKTTRRNKKTRNGLCRLDRNRTTVDYPERNTREP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Salivary chemokine-binding protein which binds to host chemokines CXCL1, CXCL2, CXCL3, CXCL4, CXCL5, CXCL6, CXCL10, CXCL11 and CXCL13.2. Include a signal peptide as an essential component of the protein.3. The protein should have C-X-C chemokine binding and be active in extracellular region. The designed protein sequence is ",
    "response#1": "EWSN",
    "response#2": "GWS",
    "response#3": "SMSPQTETKASVGFKAGVKEYKL"
  },
  {
    "reference": "MDYVNRNLDDKLLCGICGKYFSDDESLREHRRQRHMTCHMCLLCNRRIPENETLREHMKNKHNIWKLFICVCCNWSFGTEIYLKCHEECMKSTGRPGLLKPLAMPMTAPAREASALNTDPQNGSDDVPHSSPSPVPMIAENSIIQASSLKMEPIESADRSSASTSTPRTLVSGTPREKIPCGFCGKDFFHEGSLREHRRRFHMTGHTCLLCNRQIPENETVRDHMKSQHNIKKVYNCLCCNWTFLNQVHLISHKTCLKQTGKPCCRPGHMEPLAIPRTASIRQFFTLKTETQSGDDDSAAGSQLFSARLSCKSCGKFFYSERSLSKHHRQIHMSGHVCVLCNHQMPKTVTVQEHMEKEHNIRLVFNCRCCNWSFATRRCLMSHVECLKKAGDARNVKPVAIPRMAADSILQSLKESEAQEYPDFSAASTTSSGPASTLKTPRMDFKKNLKICTDAVQILVGNGLFSNEQLAQTETWVMIFSNANKLFHSMNSFGEPSVSRDIPM",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Possible transcriptional regulator (Probable); Involved in promoting segregation of chromosomes during meiosis, perhaps acting downstream of the let-60 RAS / mpk-1 MAPK signaling pathway.2. The designed protein should have DNA-binding transcription factor activity, RNA polymerase II-specific, metal ion binding, sequence-specific DNA binding to facilitate regulation of transcription by RNA polymerase II, positive regulation of meiotic cell cycle. The designed protein sequence is ",
    "response#1": "KAIGNDIYMPAEFAACKFSLRSGRGPVYSSHINPGQLIFGQASDAQHHTSTTHQLPLPPKAMGESNMGSLYVNIVKYLRDCQGAGNPLVVFTTAELMPVVSGCFRYLQSDSDEVGEQIHVYDILYLFYVLKKEVMDIADLPHANINKCITDNFFFNDFFEYYSNIACQFHEDNDRGKYCTHSMVSRWCYTFCDYMCGDLAIKPLAGKHMPPVQC",
    "response#2": "KAIGNDIYMPAEFAACKFSLRSGRGPVYSSHINPGQLIFGQASDAQHHTSTTHQLPLPPKAMGESNMGSLYVNIVKYLRDCQGAGNPLVVFTTAELMPVVSGCFRYLQSDSDEVGEQIHVYDILYLFYVLKKEVMDIADLPHANINKCITDNFFFNDFFEYYSNIACQFHEDNDRGKYCTHSMVSRWCYTFCDYMCGDLAIKPLAGKHMPPVQC",
    "response#3": "CEQRYLPCEIGCVKYSLQDGIIADFHCFIDPGEVPRGFRFHCQTASDATHKIPISCSELSNASYSVVLRGLYSFIQPRGDMWPPVYCKSEDWFRVNWCLKRMAKESGIVNLLNLFPVEDLVVKLYYQKLQKEPSKTWVCNQLDVFMWDYSSNTRCKWHEENDILFCALASCKKIAYCISNSLASMYGIALTTAHLPLQ"
  },
  {
    "reference": "MSKIQNIICSVDEVRALENDQICAGRPAKAMMENAGRAAFQLLRQCWPHAERISVYCGGGNNGGDGYVLARLAAESGFPVDLVALASPATPEARQAEESARAAGIYPRSHSQQLLEATDVVVDALLGIGAKGALRERFSFVVSEINKCSAGRLALDVPSGLSADSGAVCGDAINADVTITFIRLKRGLMTGVGPDHCGRIVLDDLGVGLEESTEEDDPVSWGYRLSRKWVEGVLPRRSASAHKGVAGGLLVIGGGVGMAGAPVMAGRAALRSGVGRVTIGCHPDSRIAAASQMPELMVVGLGDPQELTFLLARSDALVIGPGLGQDAWAQEIYRTCCDHNVPKVIDADALNLLSRGPRAVPRAVLTPHPGEAGRLRRIDVVDVQRHRPAVVEMIAEQFSGVCVLKGAGTLIAQAGKPALACDRGHSGMATAGMGDVLSGVIGALMAQGLSGYDAAGAGVWIHGVAGERVGQRGGRIGLLATDLERGIRELRTEMDFAETSGGR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein have YjeF N-terminal domains with specific sequence motifs that are crucial for its activity.2. The designed protein should be optimized for ADP-dependent NAD(P)H-hydrate dehydratase activity, metal ion binding, NADHX epimerase activity, ATP binding activity in order to improve nicotinamide nucleotide metabolic process.3. Mg(2+) is required for the proper folding and stability of the protein.4. The protein must exhibit the following characteristics: This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX..5. The designed protein should be able to compete with endogenous proteins or inhibitors for K(+), (6S)-NADPHX binding.6. The protein should have a low Km value for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "MQTCDRITVDRYGIASLDLMDNAGHAVARFALQEFPRAANITVLCGKGNNGGDGFVAARVLAGEGRRVKVLLLGYPVDLKGDAKEMFERMEIAPALAPDEESLSSAQITDILNQSDLFLDAVVGTGFRPPLKGVAMALRNRINSLDAPVVAVDIPSGWDSDSREYRTEGAYRADAVVTFTAPKPAHVSGNLTDSAYQPIVVAPIGSPDAAIEPPDVGARWPVPAATDDKYTQGVVGVLAGSAQYPGAAILCSGAAVAATSGMVRYVGSAAADVVTHYPEVVAATGMKEAGRVQSWVVGPGFGTDTSSRDVLSQVLTSDLPVVVDADALTILSSSPELVRGRTAPTLLTPHAGEFERLTGAAPGSDRVSAVRELASTWGVSVLLKGRATVIAGPDGAVVVSDAGGSAASTAGSGDVLAGILGALLASGLDTVTAAACAARVHALAAATAAHGRGGEFAAPISASPLLGAVSDAIRTVRADTHKGTYGHALIAAGSRPMSGAGLLSARAALRGGCGLVTWAVPDRLVEPLAGRVPEAMIYGLPDEERGDWSGVAPAALSQLTVGKDALVLGPGLGRWAGDGDWLRELLAGLDPALPIVLDADSLNILADMPEASAQLCGRRAVLTPHPGEMARLAGFAVPDIQRDRIEAARRYATQHAVTLVLKGARTVVATPEGDVYVNATGNPGMATGGAGDVLAGLVGSLLAQGRTPDQAAAFGVWLAGASKRITETPRPAESNKGKFGHVLVVGGSYGKSGAPAMASLAALRTGAGLVTAAVAQPILDSVVRITPELMTIPLRAGSKGEISSSNLDDETLKELLDKKTVLAIGPGLGQSAETEAFLLGLLEKTDLPAVLDADALNILAKHPGKINGRNRLRVLTPHPGEMARLAGISTGEVQANREALAREFSMKNHVTLVLKGWRTLIAHPDGSIAINTTGNPGMAKGGSGDILTGIIAAMLAQYPHLAGEAVNAAVYLHGLAADFAVWEHDEHTLLATDTVAHLSTAFRFRS",
    "response#2": "MRKMDQLTIESFGIPGIVLMENAGRGATQILFAKFHDLTQKKVAVVAGRGNNGGDGFVIARYLAQKGIDVTVYLLTQSENIKGDAAANLKLLAPLAIPVIEIPNPKAFSANKTALVHQNVWVDAILGTGLKSEVKDYFRSVIDFMNQSGKPIFAVDIPSGLDSDTGQPCGTCICAQATATFALAKTGHILFPGAKFTGTLEIVDIGIPPHITNKYQRGHVLVLGGDTVTGASRLTACGAMRAGAGLVTLASPPDTWMIYASALTSAMVRPFNGLAGFTELLADARRNVIAIGPGANVGDATRQYVLAALAARRTTVLDADALTAFAETPRRLFDAIDGPCVLTPHEGEFDRLFTAGGDKLLRTRRAAVESGAVVLLKGADTVIAAPDGRAIVNANAPAHLATGGSGDVLTGFIAGLLAQGLDVFHAAAAAAWLHGEVANYLGIGLIAEDLPDALPHL",
    "response#3": "MKLLDTAAGRFGVSEKELMRSAGEAIYREVIAAVPDGDIAVVCGSGNNGGDGYAAAAMLAEGGRSVTVIASAPPRGESSRFFAERYASMGGETLHYADSPMQCADVIADSAVVIDALFGFSFRGQLSGDYAELTELINGSDAFVVSADMPSGVYADGGYADGCVSADVTCTFSVNKPATVSYPAYGFCGRVVLCDIGVPEEAMLVGPGDAKFALRKKGEHKGQNGKLLVIGGSEDYFGAPYLAAKAASYLVDLVYLAMPEYSARRINDPDVILRPFEGRNFGKKDVEDVLALAEGVDAVVIGPGIGTRAETKEFVLEFLRWCEKPVVIDADALKAVAEDLEAIKGRKFVLTPHAGEFRILFGKKPEGSLEEKAKLVMEKAKAVGGTVLLKGVYDIISDGNVWKYNKTGNRGMTTGGTGDVLAGLVGALLAFGNEPLRAASVGAFLNGMAGDMVKEEVGENFTALEIARKVPYAVKWVLE"
  },
  {
    "reference": "MSPEPVPPPPPPQCPGCDRAEPIAQRLEEGDEAFRAGDYEMAAELFRSMLAGLAQPDRGLCLRLGDALARAGRLPEALGAFRGAARLGALRPEELEELAGGLVRAVGLRDRPLSAENPGGEPEAPGEGGPAPEPRAPRDLLGCPRCRRLLHKPVTLPCGLTVCKRCVEPGPARPQVRRVNVVLSGLLEKCFPAECRLRRLAGQARSLQRQQQPEAALLRCDQALELAPDDNSLLLLRAELYLTMKNYEQALQDASAACQNEPLLIKGHQVKAQALSGLGRSKEVLKEFLYCLALNPECNSVKKEAQKVMCEVLFSATANVHENLTSSIQSRLKAQGHSHMNAQALLEEGDAGSSENSSEKSDMLGNTNSSVLYFILGLHFEEDKKALESILPTAPSAGLKRQFPDDVEDAPDLNAPGKIPKKDLSLQRSPNSETEESQGLSLDVTDFECALCMRLLFEPVTTPCGHTFCLKCLERCLDHAPHCPLCKDKLSELLASRNFNITVLAEELIFRYLPDELSDRKRIYDEEMSELSNLTRDVPIFVCAMAFPTVPCPLHVFEPRYRLMIRRCMETGTKRFGMCLSAEHAGLSEYGCMLEIKDVRTFPDGSSVVDAIGISRFRVLSHRHRDGYNTADIEYLEDEKVEGPEYEELAALHDSVHQQSVSWFASLQDRMKEQILSHFGVMPDREPEPQSNPSGPAWSWWILAVLPLERKAQLAILGMTSLKERLLAIRRILVIITRKMNSRQELANARERNN",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein can be targeted to cytoplasm and perform metal ion binding, ubiquitin protein ligase activity.2. The protein contains novel Lon N-terminal domains that confer a unique function or activity.3. The protein design should emphasize Polar residues, Basic and acidic residues compositional bias. The designed protein sequence is ",
    "response#1": "VGLTNLGATCYVNTFLQVWFLNLELRQALYLCPSTCSDYTKGDGIHGGKDYEPQTICEHLQYLFALLQNSNRRYIDPSGFVKALGLDTGQQQDAQEFSKLFMSLLEDTLSKQKNPDVRNVVQQQFCGEYAYVTVCNQCGRESKLVSKFYELELNIQGHKQLTDCISEFLKEERLEGDNRYFCENCQSKQNATRKIRLLSLPCTLNLQLMRFVFDRQTGHKKKLNAYIGFSESLDMEPYVEHKGGSFVYELSAVLIHRGVSAYSGHYIAHVKDPQSGEWYKFNDEDIEKMEGKKLQLGIEEDLEPSKSQTRKPKCGKGTHCSRNAYMLVYRLL",
    "response#2": "RGIQNLGATCFMSVILQSILHNPLVRNLFFSGFHTSTDCKRPTCMTCAIDDMFSSIYNSKNKSTFYGPTAVLNLMWKLSKSLCGYSQQDGHEFFVYLLDQMHTESGGGTSMPCTCPIHRIFSGSLKNVVTCLDCKKERVAVDPLMDISLDINEPTLQGCLERFVSKEKVQYSCHSCGSKNAIKQLVFDKLPPTICMQLKRFEQNNFAMSTKIDKQVSYPAFLRMRYNFNQDDVDYQLYSVVCHKGTLDTGHYIAYTYYQNQWFLLDDTTIVEVKESEVLNSQAYLLFY",
    "response#3": "WLELESDPGLFTLLVEDFGVKGVQVEEIYDLQSKCQGPVYGFIFLFKWIEERRSRRKVSTLVDDTSVIDDDIVNNMFFAHQLIPNSCATHALLSVLLNCSSVDLGPTLSRMKDFTKGFSPESKGYAIGNAPELAKAHNSHARPEPRHLPEKQNGLSAVRTMEAFHFVSYVPITGRLFELDGLKVYPIDHGPWGEDEEWTDKARRVIMERR"
  },
  {
    "reference": "MLFWMVLVVVGVLIWNFSTDFQARDTALPFSEFFAQVEQGQVDSVTLSGNEIVGALKNGDRIRTYAPPQYEGLANTLLDHDVQITAEEATSSPWATLLYSWAPVLLIIGFWIFFMRQMQSGGNKALSFGKSRAKLLSSTQKKVTFKDVAGVDEAKEELQEIIEFLKEPQRFQKLGGRIPKGVLLVGAPGTGKTLLARAVAGEASVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIVFIDELDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFESNEGVILVAATNRPDVLDPALLRPGRFDRRIIVNRPDVKGREGVLAVHTKKIPLSDDVDIRIVARGTSGFSGADIANVVNEAALNAARYNQKTVQMANFEFAKDKVLMGSERRSMIISDEEKKTTAVHEAGHALLAVLLPHADPIHKVTIIPRGMALGITQQLPVDDKHSYSREYLNDQIAILLGGRIAEEITNNSVTTGAGNDFERATELARRMVCEWGMSDKLGPLAFGKKEEQIFLGREIAQHQDYSEETAIQIDQEVKRLVMANYKRSRAILEKNRSTLVQIAEELLVREVLEGKQVQRIVAGLPLDDPQQTSGSSGTPPPVDEEALEQSSAPPLPPLNKPLPQE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have Helical domains to ensure proper insertion into the membrane.2. For general function, the protein need meet that Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins; Plays a role in the quality control of integral membrane proteins.3. The designed protein must have a active site active site that is highly conserved among related enzymes.4. The protein must bind to Zn(2+) in order to perform its enzymatic function.5. The designed protein must possess ATP-dependent peptidase activity, ATP binding, ATP hydrolysis activity to support protein catabolic process, proteolysis under certain conditions. The designed protein sequence is ",
    "response#1": "IPKGVLLYGPPGTGKTLLARAVAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFGQAKASQPSIIFMDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFDQEITVILIAATNRPDILDPALLRPGRFDRQIAVDRPDFEERMKIWLKHNL",
    "response#2": "VLLVGPPGTGKTLLAKAVAGEANVPFFSMSGSDFVEMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAIGRARGRNVNFSANDERENTLNQLLTEMDGFASNQGVIILAATNRADILDRALMRAGRFDRQIHVLNIQF",
    "response#3": "ILMVGPPGTGKTLLAKAIAGEAKVPFFTMAGSDFVEMFVGVGASRVRDLFEQAKKNAPCIIFIDEIDAVGRKRGGAGFSGGHDEREQTLNQMLVEMDGFEGSEGVIVIAATNRSDVLDNALTRPGRFDRQVMVV"
  },
  {
    "reference": "MVLIRVLANLLILQLSYAQKSSELIIGGDECNINEHRSLVLMYYDGHQCDGTLINEEWVLTAAHCDGENMEIQLGVHSKKVPNEDVQTRVPKEKFFCDSNKTYARWNKDIMLIRLDRPVSNSAHIAPLSLPSSPPSVGSVCRIMGWGTFSTTQETYPDVPHCANINIHDFEVCQAAYPGLPATNRILCAGILEGGKDTCKGDSGGPLICNGEIQGIVSWGGHLCGNVLEPGIYTKVFDHLEWIRSIIAGNTDATCPL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Snake venom serine protease that may act in the hemostasis system of the prey.2. The designed protein must be optimized for toxin activity. The designed protein sequence is ",
    "response#1": "IMGGSECYKSKHPFLVYLYNSAGFFCSGTLLNHEWVLTAAHCNRDDIQLKLGVHNVHVHYEDEQIRVPKEKLCCLSTKNCTQWSQDIMLIRLNSSVNNSKHIEPLSLPSRPPSMGSDCTVMGWGTITSPKVTYPKVPHCVDIKILHNPVCQAAYPTMSRKNILCAGVLEGGKDSCKGDSGGPLICDGQIQGIVSWGRFPCAQLLEPGVYTKVFDYIDWIRGQ",
    "response#2": "IMGGSECYKSKHPFLVYLYNSAGFFCSGTLLNHEWVLTAAHCNRDDIQLKLGVHNVHVHYEDEQIRVPKEKLCCLSTKNCTQWSQDIMLIRLNSSVNNSKHIEPLSLPSRPPSMGSDCTVMGWGTITSPKVTYPKVPHCVDIKILHNPVCQAAYPTMSRKNILCAGVLEGGKDSCKGDSGGPLICDGQIQGIVSWGRFPCAQLLEPGVYTKVFDYIDWIRGIIR",
    "response#3": "VIGGDECNINEHPFLAFVTSDRRRCAGTLINQEWVLTAAHCNGKYMKIELGVHDKMVRNEDKQTRVPKQKFFCLSSKEYTMWDKDIMLIRLNTPVNNSTHIAPVSLASRPPVVGSVCRIMGWGTISSPKVILPDVPHCANIEIIKYSKCQGVHPELPAKGRVVCAGIWQGGKDSCHGDSGAPLICNGQLQGLLSWGGDPCAQPLQPGLYTDIFDYSDWIQSIIR"
  },
  {
    "reference": "MTLEITGSELIKSKLIDAPERSGVYRMFDVNKQVLYVGKAKNLKKRLTNYIKSNLDNKTLRMIANTCFLEYSITNSEVEALLLEAQLIKKFQPKFNILLKDCKSFPFIKLRLDHDFPQLLKYRGKTLSDGKFFGPFASSAEVNTTLTELQKIFKLRSCTDNYFNSRTRPCLQYEIKRCYAPCVGKINKEDYRDLVTQVKDFLQGRTKELQENLSRKMEELSSQMRFEEAAEIRDRIKALSYVQLKAGVSDVVKDADIIAIVEKNGHYCVEVFLYRAGQACGNIPYFPTSTENSTKEEVLEYFLLQFYQKQHVPAAIIINHEINDKENVIEAIKKINNILQINITVPNKGGKAKLVQNAETNALFSLEQYLKKFAKNQEIMFEIKELFGLSEIPERIEIYDNSHIQGKFAVGVMVVAGKVGFDKKEYRVFNVHAPSLVCHSRESGDPKRLMDSCFRGNGIKNCGGDIKGDDYEMLRQVLTRRLTRLRQEPHKLPSLMIIDGGKGHLGVVKEVMDKFEMNIPFVCMSKGVDRNAGFEQFHVIGKEVFTLDKNLPVMKYLQILRDEAHNFAIKNHRLGRSRAIKISRLDDIEGVGETRKKALLHYFGSYKAVCDATIYELAKVNGINKLLAEMIFNVLHRKN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that The UvrABC repair system catalyzes the recognition and processing of DNA lesions; UvrC both incises the 5' and 3' sides of the lesion2. The designed protein should be involved in SOS response by localizing to cytoplasm and exhibiting excinuclease ABC activity, DNA binding. The designed protein sequence is ",
    "response#1": "SPYKPLGDQPKAINSLVDGINKGEKEQTLLGVTGSGKTFTMANVIEKVQKPTLVISHNKTLAAQLYEEFKEFFPDNAVEYFVSYYDYYQPEAYVPRTDTFIDKESSVNEEIDIMRHSATQSLLSRDDVIVVSSVSCIYGIGSPEDYGEFAFGIAVGDNYDRSDIIRKLVFMQYERNDIEFARGHFRVRGDVIEINPVHGTPPVRVELFGDEIDAISLIDKVTGKKTESLKRYMIFPAKHFVVGQDKMDTAIRNISDELDERLNEFNLSNKLLEAQRLEQRTRFDIEMLQEMGYCPGVENYSMHLSGRKWGEKPYSLLKYFPEDYLTIIDESHVTLPQIRGMYNGDRARKETLVEHGFRLPSAKENRPLRFDEFESSINQIIYVSATPGAYELSRSSNIVEQIIRPTGLVDPEVIIPTGDQPQAIEKLVAGLRAGYRHQTLLGATGTGKTFVMAHIFAQIQRPTLVLAHNKTLVSQLWAEFREFLPDAAVEMFISYYDEYTPEAYVPSKDLYIEKEASINEEIDRLRHAATQALLTRRDVLAVIACVSDGVTACVTVFFFRQGRNLGNKVFYPRIPEGADEAEVLAAFLARYYIGRKAPPELVISHTIPEQKVLGEALSRESGHRVRVTHSVRKERRRWLEMALTNARHALTARTSTQAMALHRLEALQDALQLPALPERIECFDISHTRGEATVAACVVFNQDGPLKSDYRRFNIRGITPGDDYAAMRQALSRRYQRLKKGEGVLPDILLIDGGKGQVAQAEAVLA",
    "response#2": "DGINKGEKEQTLLGVTGSGKTFTMANVIEKVQKPTLVISHNKTLAAQLYEEFKEFFPDNAVEYFVSYYDYYQPEAYVPRTDTFIDKESSVNEEIDIMRHSATQSLLSRDDVIVVSSVSCIYGIGSPEDYGEFAFGIAVGDNYDRSDIIRKLVFMQYR",
    "response#3": "GDQPAAIEELARRIQGGAADTVLLGATGTGKTATVAWLIERLQRPTLVIQPNKTLAAQFANELREMMPHNAF"
  },
  {
    "reference": "MHINEETDWIDLVKPALAKKPKKIVDNSDKFPTPRGFQQKSLVSKNIHSGNSASSTSIFAKREEELQKDLLLKKAWELAYSPLKQIPMNAILAYMSGNSLQIFSIMTTLMLLVNPLKAITSTGSAFTPFKGTHPGTLWPAMGAYILFQLLLMGIGVYKLQRMGLLPTTTSDWLAWEVSKVFMDRSYGPSKTVL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The designed protein should be expressed specifically in the endoplasmic reticulum The designed protein sequence is ",
    "response#1": "RFKVQGRQKLEVDGPCVIISNHQSILDMMGLMEILPKRCVQVAKRELLFLGPVGLVMYLGGVFFINRQRSSTAVSVMANLGERMIEENLKVWIYPEGTRNDSGDLLPFKKGAFYLAIQAQVPIIPVVYH",
    "response#2": "SGNECESADWLNALLIQVLEAYRVKLRDGLPGAEGDEVARKRVERFANQMRPPGFLDPIKVHSVDLGVTAPRLSRARPRPQKAPNTDPAIEFDMSYADTISLSLSTSVLFNYPFASFARLPVSLTISLSHFSSSVLLTPPQPHAQHPTVTLNLPSPGTDFVLNIQTKSLMGSRAKLADVPKLHEMITHQIQRVLLEA",
    "response#3": "GLRFKVQGRQKLEVDGPCVIISNHQSILDMMGLMEILPKRCVQVAKRELLFLGPVGLVMYLGGVFFINRQRSSTAVSVMANLGERMIEENLKVWIYPEGTRNDSGDLLPFKKGAFYLAIQAQVPIIPVVY"
  },
  {
    "reference": "MANNNVYSVLKSLLLLLHLVFLSKQHVDSASIVKFLPGFEGPLPFELETGYIGVGEEEEVQLFYYFIKSERNPKEDPLLLWLTGGPGCSAISGLLYQNGPLAMKLDVYNGTLPSLVSTTYSWTKTSSMIFLDQPVGTGFSYSRTQLFNKPSDTGEAKRIHEFLQKWLGKHQEFSSNPFYVGGDSYSGLVVPATVQEISKGNCQCCNRPINLQGYVLGNPLTDCVYDCNYRVPFAHKMALISDELYESLKRTCRGEYVNVHPHDTECLKFVEEFNKLTNRVCERHILHSCCETETPSCYSYRFMLTTYWANDETVRKALQINKESIGEWTRCYRGIPYNHDIKSSVPYHMNNSIDGYRSLIYSGDHDIQVPFLGTQAWIRSLNYSIIDDWRPWMIKDQIAGYTTSYVNKMTFATVTGGGHTAEFTPKETFMMFQRWINGQPL",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Probable carboxypeptidase.2. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. A protein that can perform serine-type carboxypeptidase activity, acyltransferase activity, transferring groups other than amino-acyl groups in extracellular region to support proteolysis, secondary metabolic process. The designed protein sequence is ",
    "response#1": "VVGDSISAALGLDTSQGWVALLQKRLADEGYDYRVVNASISGDTSAGGLARLPALLAEEKPALVVIELGGNDGLRGMAPAQLQQNLASMAQKARAEGAKVLLLGIQLPPNYGPRYIEAFSRVYGAVAAQEKTALVPFFLEGVGGVQGMMQADGIHPANQW",
    "response#2": "VVGDSISAALGLDTSQGWVALLQKRLADEGYDYRVVNASISGDTSAGGLARLPALLAEEKPALVVIELGGNDGLRGMAPAQLQQNLASMAQKARAEGAKVLLLGIQLPPNYGPRYIEAFSRVYGAVAAQEKTALVPFFLEGVGGVQGMMQADGIHPS",
    "response#3": "VVGDSISAALGLDTSQGWVALLQKRLADEGYDYRVVNASISGDTSAGGLARLPALLAEEKPALVVIELGGNDGLRGMAPAQLQQNLASMAQKARAEGAKVLLLGIQLPPNYGPRYIEAFSRVYGAVAAQEKTALVPFFLEGVGGVQGMMQADGIHPD"
  },
  {
    "reference": "MPTLRDSTMSHPGENSHQVRVKAYYKGDIMITHFEPSISYEDLSGEVRDMCSMDNDQQFTMKWIDEEGDPCTVSSQLELEEALRLYELNKDSELTIHIFPCVPEKPGMPCPGEDKSIYRRGARRWRKLYYASGHAFQAKRFNRRAHCAICTDRIWGLGRQGYKCINCKLLVHKKCHKLVTVECGRPMIQVRSVTNHHNLSEPAKNQSCPAHGQGTGKSPTSLGLADFDLLRVIGRGSYAKVLLVQLKKTERIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTDSRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSNENPDQNTEDYLFQVILEKQIRIPRSLSVKAASVLKGFLNKSTKFANNQIVIFVFSSFQLEQKQVLPPFKPNISGEFGLDNFDAQFTNEPIQLTPDDEDVVRKIDQSEFEGFEYINPLLMSAEECV",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be able to bind ATP ligand in a variety of conditions2. A protein that can perform calcium-dependent protein kinase C activity, protein serine kinase activity in cytoplasm, zonula adherens to support neuroblast proliferation, pronephric nephron tubule epithelial cell differentiation, protein phosphorylation.3. The designed protein should contain one or more AGC-kinase C-terminal domains that are essential for its function.4. The designed protein must have a Proton acceptor active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "STYSFSVSRKAHRYAQVGKVCVEHCWELSGVRVQYKLQPSGVNCSALGVLSSAKDTSGTLFVNDTEALQRPECDELQYTVVATDQETHAQAQAALLVTVEGC",
    "response#2": "FEMGAHLGRGKFGRVYLARERHSHYLVAMKVMFKEELRKGCVQRQVLREIEIQSRLKHPHILRLLTWFHDESRIYLALEIASEGELFKHLRGAPNHRFDEPRSAKYTYQVANALNYCHLNNVIHRDLKPENILLTSTDDLKLADFGWSAHTPNNKRRTLCGTLDYLPPEMVDGNSYDDSVDQWCLGILCYEFVVGCPPFESNSTESTYSKIRRMEISYPSHLSKGCKELIGGLLRKESKGRITLVDVMTHYWI",
    "response#3": "GVKAGFICVGSSFPTNSSYQKNRDSLFSTLSDKVTTNGGFYNASLDGVHVVGLCRRDYDRQGCINCVEESIRQIKTSCSNRVQSFHCNSDDRERVSCLVRTTDQSC"
  },
  {
    "reference": "MLTLYSETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRRAGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIGDDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTGLGAVHAYALNVLRERLPYTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVNMARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that is capable of catalyzing reactions within the Cofactor biosynthesis pathway with high efficiency and specificity.2. The Schiff-base intermediate with DXP active site of the designed protein must be optimized for substrate binding and specificity.3. The protein must exhibit the following characteristics: Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS.4. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "FYGHEFSSRLLLGTAQYPSPEILRKVIDKSGTEIVTVSLRRETAGGKHSGQFWQFLKELNVTILPNTAGCYTIKEAVTTAQLARDLFKTPWIKLEVIGNPDTLQPNIFALVEAAQILNNEGFQIFAYTTDDLIVAEKLLEVGCRVIMPWCAPIGSAKGPRDTDGLRSIRAYLPDLTLVIDAGIGRPSHATIAMELGYDAILLNTAVAKSGDPVLMAEAFSKAVNAGYMAYKAGILEARDVAVPSTP",
    "response#2": "MRILLGITGGIAAYKAAGLLRALSELGHEVTALPTENALRFIGDATLEALSGKNIDPDLYGDVASVRHVQLGQEADLIVVAPATAAFLGRYASGIADDLLLNALLATHAPVVVVPAMHTEMWQNAATVENVETLRARGVRVMEPASGRLTGEDSGPGRLPEVEDIVSFLE",
    "response#3": "RVTLGVSAGISIYKACDLVRRLMDRNCEVQVVLTSHAARMVAPVTFQALSGRPVITDLFEGAGEPSIAHVELARWEELLVVAPATANVLGKFARGIADDFLTTHYLANRGAPVLLAPAMNGAMWRHPAVQENLRILEQRGHAVLQPGKGSLACGDVDEGRLPEPAAIAQEAVEAFVR"
  },
  {
    "reference": "MEEIKKRIIFLIDKINKTNYEYYTLNESSLNDQQYDALFRELLYLESKYPEYNYKFSPTTKIGGPISSKFHKFLHEKSMLSLNNVFNIKELKLFYDRISKKISNFTLLTELKIDGLAVSLKYKKGILDKAITRGDGYQGELITDNVKTIKELPLKLKEPLDLEVRGEVYMSYESFNYLNQIRKKENKSLFANPRNAASGTLRQLDSKVAAERNLSIFLYTIVNPPKFIITQKSILEFLTYLQFPVNNYYEYVLNWDQLIKKISYYEEIKNHLGYNTDGIVIKINELSFHSIIGYTSKAPKWAIAYKFKVFKTETLINNILFQIGRTGIVTPIAELLPTIVDGSVISKVNLHNFDYIKQKDIRVNDFVLVHKSGSIIPEIIEVIKTKRKNQIPFEMITHCYSCNTKLIKKDSNHFCFNLDCEEKKIQELFYFVSKSAMDINVLGLQTLKILFYKGFINNISDLYSLNQYKKEVEELHGFGKKKFNNIIISLEKSKNKCLSNFLIGLGIKNVGIHLAKILAQKFENIDNLQKASIESLLKIDEIGIKSAQNIKNFFLNSKNLKLIEKFKNLGLNLFYFKSKNNIKNNIFKNKKVIFTGILEKYSRNQAQNIVIELGGVIINSITNKTNYLILGKNPGSKLLKAKKFNIKVLQEHEFEELIK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double- stranded DNA using NAD as a coenzyme and as the energy source for the reaction; It is essential for DNA replication and repair of damaged DNA..2. The designed protein is the optimization of the N6-AMP-lysine intermediate to achieve high catalytic efficiency for NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-   (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-   nicotinamide D-nucleotide..3. The protein requires Mn(2+), Mg(2+) to activate a critical catalytic site.4. A protein with DNA ligase (NAD+) activity, DNA binding, metal ion binding functions is required to enable DNA replication, DNA repair.5. The designed protein should be able to compete with endogenous proteins or inhibitors for NAD(+), Zn(2+) binding.6. The protein contains novel BRCT domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MSKKRHEELKKIISEHDHNYHVLDKPTITDYEYDQLFAELLDIEKNPKGLDLSDSPSQRVGGTVLEGFTKAQHRLPMLSLANSYSPEDIFEFDERVRKFLNTEDPVEYLCELKFDGLSMELIYENGQLVRAITRGDGTVGEDVTHNIKTIKSIPLKLSHKNPPPLLEVRGEVLMFKEDFARLNETQQENGQQTFANPRNAAAGTVRQLDSRIAASRPLRFFGYALGAVEGETFNTQKNIQEYFNDHGIPTVLPYKEDLLVVAKGPEEVVKYYHHIEKVRPKLPFDIDGVVIKVNSLRLQEDLGLVARSPRWATAAKFKPEQAQTTVEDIVVQVGRTGALTPVAIMKPVKVGGVTVTNATLHNQDEITRKDIRIGDTVIIQRAGDVIPEVVEVVNPDKRPADRLPYSIPERCPACDSVAVKAEGEVVTRCVNPLCIAVVKESLKHFVARRAMDIDGLGVKQVEQFYEAGLIHNYADLYKLKKDDWASLPRMGKKSAHNVMTAIADSKRPSLARVFRSLGLPYVGEGTATRIARVYRSLEDIRQASVDDLARIPDVGRQVAEGIVQGLRDAAFDELLASGITPLFTPRQPLNGESWVVTGTLETMGRDEATQRLQALGARVSGSVSSKTKCVVAGEKAGSKLDKAEKLQIRVMNEQEFLAFLAQK",
    "response#2": "MKKEEYLEKVALANLWMRAYYEKDEPLASDEEYDALIRELRVFEEQNKDEISKDSPTQKIAPTIQSEFKKIAHLKRMWSMEDVFDESELRAWAKRAKCEKNFFIEPKFDGASLNLLYENGKLVSGATRGDGEVGEDITLNVFEIENIPKNIAYKERIEIRGEVVILKDDFEKINEKRALLNQSLFANPRNAASGSLRQLDTSITKERNLKFYPWGVGENTLNFTKHSEVMQFIRELGFLKDDFVRLCANLDEVLKAYDELLALREKKPMMMDGMVVRVDDLALCEELGYTVKFPKFMAAFKFPALEKTTRLIGVNLQVGRSGVITPVAVLEPVNLDGVVVKSATLHNFDEIARLDVKINDFVSVIRSGDVIPKITKVFKERREGLEMEISRPKLCPTCQSELLDEGTLIKCQNIDCEDRLVNSIIHFVSKKAMNIDGMGDKVVEQLIKKQLVSYPSDIYRLNYEDLIKIEGVGGKVAQAVVEAIEKSKKTTLARVFRSLGLPYVGEGTATRIARVYRSLEDIRQASVDDLARIPDVGRQVAEGIVQGLRDAGVTLAEIIDAPLLGKTVVITGTLPSLSRAEAQKRLETLGAKVTSQVSRQTDFLLLGADAGSKLARAQAFSVPIIDEAQWEKLIEI",
    "response#3": "MKKEEYLEKVALANLWMRAYYEKDEPLASDEEYDALIRELRVFEEQNKDEISKDSPTQKIAPTIQSEFKKIAHLKRMWSMEDVFDESELRAWAKRAKCEKNFFIEPKFDGASLNLLYENGKLVSGATRGDGEVGEDITLNVFEIENIPKNIAYKERIEIRGEVVILKDDFEKINEKRALLNQSLFANPRNAASGSLRQLDTSITKERNLKFYPWGVGENTLNFTKHSEVMQFIRELGFLKDDFVRLCANLDEVLKAYDELLALREKKPMMMDGMVVRVDDLALCEELGYTVKFPKFMAAFKFPALEKTTRLIGVNLQVGRSGVITPVAVLEPVNLDGVVVKSATLHNFDEIARLDVKINDFVSVIRSGDVIPKITKVFKERREGLEMEISRPKLCPTCQSELLDEGTLIKCQNIDCEDRLVNSIIHFVSKKAMNITGLGPSLVEKLFRKELIKDVADLYYLKKEDLIKIEGVGGKVAQAVVEAIQKSKSNSPERVFRSLGLPYVGEGTATRIARVYRSLEDIRQASVDDLARIPDVGRQVAEGIVQGLRDAGGILEKLKELGVNPTLKETQESKKLQGLNFVITGTLSKPRDFFKELIIKNGGNVLNSISNRINYLLTGEKSGSKIEKAQKINTIKILDENEFNKL"
  },
  {
    "reference": "MKVVLGVSGGIAAYKACELLRLFTESGHEVRVVPTREALKFVGAPTWAALSGNPVSAEVWDDVHEVPHVRIGKQADLVVVAPTTADVLARAAHGLAGDLLTNTLLTATCPVVFAPAMHTEMWQHPATRANVATLRERGAVVIDPAVGRLTGADTGPGRLPDPAEIFEVCLRVLRGRPRDLAGRKVVVSAGGTREAIDPVRYLGNRSSGRQGYALARTAVARGAEVTLVAANVTLPDPAGAKVVRVESASELRDAVLAAAADADVVVMAAAVADFRPATRHEAKIKKTDGEPEPIFLAKNPDILAELGTIRREARAARTRAGGTKPPLPAATVPDPAAAGPDLAGAATGPEPAAVGPEPAGATTGPESAAAAPELAVAGPESGDFVARPERGAYPEVIVGFAAETHDVLANGQAKLARKGCDLLVVNQVGEGLAFGTTDNAATVLVAGGDPVEVPFGPKEALADVVWDLVAARLP",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Catalyzes two steps in the biosynthesis of coenzyme A.2. A protein with phosphopantothenate--cysteine ligase activity is needed to enhance coenzyme A biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "KKIILGITGSIAAYKAALLVRLLRKQDAQVRVVMTTAAKEFVGELTFSNLSQKPVFSGLWDGSWTEHVHLGGWADLMVVAPASANTLGKMAHGLCDNALTAVYLSAKCPVLVAPAMDADMYQHPSTLRNLDQLKADGVTVLDAGTGFLASGLHGPGRMAEPAQILEAIIHEDRRVVITGGGTREKIDEVRYISNFSSGKMAKAIALSLYLKGADVCYITTKGNDSLPKSIYTIEVDDAQEMLEYTQDALRIAKKGKLSKASLTTADAIKLIQKRPFLFMVAAVADFTPKFPQQGKLKKSTLGENWQIELTQTSDILSTLAKDREGVTTIAFKAEMDTQEGLNNAKELLTKKEVDAVCYNLLEDAKSFGGDQNSITFLEDGERLIAEALKDVGPCSMFYLAAAVSDFYVPWKSMTEHKIESGSGPLDIRLAQVPKMLSVLRSNWAPKAFCISFKLETDSKILMEKATKALRKYKVHAVVANELSTRKRLIAEALKDVGPCSMFYLAAAVSDFYVPWKSMTEHKIESGSGPLDIRLAQVPKMLSVLRSNWAPKAFCISFKLETDSKILMEKATKALRKYKVHAVVANELSTRKKAEELKLLGLTVFSL",
    "response#2": "KRILLGVSGGIAAYKSVTLASRLTQANALVDVIMTESATKFIAPVTFQAITQRPVSTDIFDSWSDTGRLRIGHVALAHAADLFIIAPATAQTLARLALGMADDLLSITTLSYKGPMLIAPAMETNMWHHPATQEHVAKLRGWGVDFVGPAEGRLASGEYGAGRVVEPEEVFEAAGL",
    "response#3": "KHIVLAVTGGIAAYKACALTSKLVQAGAVVKVMMSESAKEFVNPNTFQALSRNPVYDWTFHEPDPSKIAHIDLADWADLVVVAPATANVIGKLANGIADDMITTTLLATKADVYLAPAMNVNMYNHSAVQKNMQTLKDFGYSFIEGEAGYLACGWTGKGRMAEPEVIANMEAGLEDRRVVITGGGTREKIDEVRYISNFSSGKMAKAIALSLYLKGADVCYITTKGNDSLPKSIYTIEVDDAQEMLEYTQDALRIAKKGKLSKASLTTADAIKLIQKRPFLFMVAAVADFTPKFPQQGKLKKSTLGENWQIELTQTSDILSTLAKDREGVTTIAFKAEMDTQEGLNNAKELLTKKEVDAVCYNLLEDAKSFGGDQNSITFVTKDEVKEIPRADKGELARLILERLA"
  },
  {
    "reference": "MIRQRTLKSIVQTTGVGLHSGRKVTLILRPAAANTGVIYRRTDLNPPVDFPADANSVRDTMLCTALVNEDGVRISTVEHLNAALAGMGIDNVIIEVDAPEIPIMDGSASPFIYLLQSAGIDTLNAPKRFLRIKKPVRIEDGDKWAELVPFNGFRLDFTIDFNHPAIDADQQRLVLDFSSQSFIKDISRARTFGFMRDIEYLQSQNLALGGSFDNAIVLDDYRILNDDGLRFDNELVTHKVLDAIGDLYMCGHNIIGEMRAYKSGHALNNQLLRAVLADQEAYEWATFQAEEVPVTFAQPGMVLA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein should be able to manipulate the activity of enzymes in the lipid IV(A) biosynthesis, Glycolipid biosynthesis, lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 2/6 pathway, either by activating or inhibiting their function.2. The protein must exhibit UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity, metal ion binding.3. The designed protein should have a binding site that is specific to the target Zn(2+) ligand.4. The protein with the reaction a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine +   H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate, the Proton donor must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MSKPLRPSPADPAPVTLRDVARLAGVSPMTVSRVLNGKAGVSDETRQRVLDAVAALGYRPNAFAQGLATKKSRMIGVVVPSIDNPIFADTVRGIEDTLSDAGYSMLLASSGERAERERAHLAMLAEKRVDGLLMVSAETHDELLRQAASKLPMVQVARRVPDGTVASVGFDDRAGARLAVAHLVGLGHRRIAHLAGPADWMSSRARRDGFVAAMRESGLAPDERLVAEGDYTPESGYRAGQLLLAREPRPTAVFAANNLMTLGALRAVQDAGLRVPEDVSLVGFDDIELASYVTPALTTIRQPKYEMGVTAARMLLDMIEGKAPDREVVLPTELVVRGSTAAP",
    "response#2": "MNDRATSGGARPAMLREVAAAAGTTIEAAAAPADKAPPVPAAPAGSAGPGIAAANAAADTATNTPANTAANAPANTAADAPANTAANAPADAAADATAAGAAGDPAPLADFAVDTRRVGPGDIFFAYRGLTFDGRDFVADAAARGAAAALVEWDGPRLAGETAPGLAGSDAPVHAVPNLRTKAGDLAAGWYHEPSARLAVFGTTGTNGKTSVTHFLAQALTALGRRCGMTTTNHIRIGTAELPADLTTPDPVTLHRQLRRFRDAGAEAVAMEVSSHGLDQHRTAGLDFDAAIFTNLSPDHLDYHGTMEAYRDAKLKLFDGLKNGRPFAVVNADDAWGRELAARTRARVVTFSRATAADIRAEHIGLAADGATFTARGPWGDLKVRVPLVGRFNVYNALAAVACAAAEGIAPETAAAGLAEAAVPAARLEAVAAPAGAPGAPGAPGAPGAPAAPGAPDAPDAPDAPAAAAASDAGAEAAAAGADSAGAAAEAAPAAAAEPAAGAEGAEAAGAGAAA",
    "response#3": "MTLSELLSPLAGWKLLGDSDAXGXAXDLXVXGXGXPXGXQSHAXGXGXGXAXXVXXDLXXAXGXXXAAXSXRXLAXAXWXPAXITGITAXEXPXVXVXSXAXRXVXXXSXTXTHAAXGEXVXPXQXAXGXHXAXAXAXK"
  },
  {
    "reference": "MKLSQLLEHLQYEVVQGSDDIPVTELVNDSRKVCDGSVFVCISGAVSDGHAYIAEVAEKGAKAVVVERDIDAPESLSVIKVTDTRYALALMSAAYFGYPADRLKVIGITGTKGKTTTTYMVKSILEGVGHKVGLIGTIEAVIGDKVIPANNTTPESFVIQKYFAEMVEAGCDSVVMEVSSQGLMLHRTAGIPFEIGIFTNLGEDHIGPNEHKDFEDYKRCKGLLFKQCKLGIANVDDEWYEDVFAGATCKVETFGLSEKAGLRAEDIEHISRPGYLGVKYRVSGLMDFHVEIDIPGEFSVYNSLTAIAVCRHFDVPVENIKAALKEAKVKGRIEMVKVSDEFTLMIDYAHNAMALESLLHTLRDYNPGRIVSVFGCGGNRSRTRRYEMGEVSGRLADFTIITSDNPRFEEPQEIINDIITGMKKTDGKYIDICDRGEAIKYAIAHGRPGDVIVVAGKGHETYQEIKGVKYDMDDRVLIREALKEIAEGR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein contains novel Mur ligase C-terminal domains that confer a unique function or activity.2. A protein that can perform magnesium ion binding in cytoplasm to support cell cycle. The designed protein sequence is ",
    "response#1": "VTGSNGKTTVCALLDSILSHARLSVGVIGTTGTRYPGMQGKPGLTTPDPVTLQATLKDMLDAGCDAVALEASSHALDQRRLDGLQPVSAGFTNLSRDHLDYHGDMESYYLAKKRLFSGLHPQTACLNLGDASGRRLAAELAGGPILLGYAVDGAAQAGARILPRLAALNPQFDANGCRFTLQDAFSDATVEATLPLPGRFNLDNALCAAAMLA",
    "response#2": "VTGTNGKTSISTFVRQIWTELGHMAVNLGTTGVEGAWTAPLAHTTPEPITLHRCLAEAAEAGVTHAAMEASSHGLEQRRLDGVHLTAAGFSNFTQDHLDYHATFEAYFAAKAGLFDRVLPQDGTAVINMDDPKGVAIRDIAQARGQTVLTVGSGDADLCLLNQRFDATGQDLRFAWRGKAFQTRLNLIGGFQAENVLLACGVTGTNGKTTVTTAIYLALDNCGIKSGLIGTIEYRIGESSIPAKFTTPEAYDFFSLLRRMRDSGCAFASIEASSQALVQRRVYGEVFDIAVFTNLSVDHLDYHGDMESYYSAKKLLFSSAKKAVINIDDKYGKRLSRELDGDVVTFSVCEEADYNAEGITHSVEGTKFVLKGKYFKESVSFPISGDFSVYNAAMAAIARELGMPEEAIVKGLAEFGGVKGRFETIRSAKGITAIVDYAHTPDALTNVLEAIHEVLQGKGKLLTVVGCGGNRDRAKRPLMAIEAVRLSDRALFTSDNPRFEEPEAILQDMLDGLSEEQQAGVLTIVDRREAIKTACMLAKPGDVVLIAGKGHEDYQEINGVKHPFDDKEVLTELLEQA",
    "response#3": "VTGTNGKTTTAYLIESVIASAGFISGVIGTVNYRYPGKIVRGSNTTPDPIVLASLLRDMADTGVTHAVMEVSSHALDQRRVDGCPFRIAVFTNLSRDHLDYHDSMDSYFKAKTRLFQEFEYAGGLPPYAAINADDRRGKELIGMLEKSRRTGVESGIITFGLSEGCMVRPRSHSIGIEGIRALISTPACELEIESSLIGRFNLYNILAAVSL"
  },
  {
    "reference": "MSGNTEELKNWFEFRQKVEAGEVKSIEKVGRDFYGEYASTVVVKNNVKGFHIQGDERLSEEDMKWLYAAAEKHPHLTLNFQERSQWPLLLINFIPWIILLFLIYFFFIRQLRGPGGPGGVLSFGKSRATLITRDKAKKTFKDVAGIEEARSEVEELVGFLKNPRKFQRLGGRIPKGVLLIGPPGTGKTLLAKAIAGEANVPFYSISGSDFVEMFVGVGASRVRDLFQQARENSPCIIFLDEIDAVGRRRGTGLGGGHDEREQTLNEILVQMDGMESDDKIIVMAATNRPDVLDPALLRPGRFDRHVYVDLPDVKGREQILRVHARKVKMSDGADLSVLAKTTPMFSGADLENIINEAALIAVMKDKESIDNQDLEEARDKVVWGREKKSRVLDEADRWVTAYHEAGHALLGYLVPDVDKPHKVTIIPRGPSLGATHFLPSKDVYNRTSKELIGQIKVAYGGRIAEEMFIGDISTGAKGDIDQASAIARKMVCEWGMSDRLGPIKYQEDEETVFLGREITKTQFHSPETLRLIDQEVRKIIDDAYAAAREIIERHRTEIECIAQALMEYEVISGEELAALVKDRKLPGDRKPIKQVAPRVQRSKDEPAKRDSSGEPVVPSGDASLPSPSPA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The protein contains novel AAA+ ATPase domains that confer a unique function or activity.3. The presence of Zn(2+) is crucial for the protein to function optimally under specific conditions.4. The protein design should emphasize Basic and acidic residues compositional bias.5. The ideal protein would be one that plays a critical role in protein catabolic process through zinc ion binding within plasma membrane.6. The protein must exhibit the following characteristics: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. The designed protein sequence is ",
    "response#1": "IPRGVLLYGPPGTGKTLLARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFKQAKENSPCIIFIDEIDAVGRARGSGMGGGHDEREQTLNQLLVEMDGFGDRGSVILMAATNRPDVLDPALLRPGRFDRQIPVTAPGAEERRRIAGVDLKVLAKGTPGLAGAELANMVNEAALLAARKNKTKVGMEDFEAAVDALDG",
    "response#2": "VLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAKKAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNESIIVIAATNRSDVLDPALLRPGRFDRKVLVLFLDPPDFETHAKILELAAAEGKNVDEVSAQKIAAGTPGMAGADLENLVNEAALLAARKNHDYVTTEDFERCIDALLGPAKS",
    "response#3": "VLMVGPPGTGKTLLARAVAGEAGVPFFHISGSEFVEMFVGVGASRVRDLFEKAKAAAPSIVFVDEIDAVGRQRGAGLGGGHDEREQTLNQILVEMDGFDNQTNVIVIAATNRADILDPALLRPGRFDRKVFVLFTDVPESEGLGRIEESYLFPSRELNVEVKTLARGTPGFSGADLANLVNEAALLAARRGRRLVTMAEFQRALERVIVEKEKLIVAYHEIGHALVQWVLPYTEPVHKVTTIPRGMAALGYTMTLPTEDRYLKSKNEYLSEIRTLLGGRASEEVVFGDITTGASNDIERATAIAHAMVTKFGMSEKFGPILLDNTNDGDLFMQKHYSETTGKEVDDEVRTLITEAYEDSKKILRDNYEKLEKVTRALLDRETISGIERLLGRPL"
  },
  {
    "reference": "MTGKKSSREKRRKRSSQEAAAALAAPDIVPALASGSSGSTSGCGSAGGCGSVSCCGNANFSGSVTGGGSGGSCWGGSSVERSERRKRRSTDSSSVSGSLQQETKYILPTLEKELFLAEHSDLEEGGLDLTVSLKPVSFYISDKKEMLQQCFCIIGEKKLQKMLPDVLKNCSIEEIKKLCQEQLELLSEKKILKILEGDNGMDSDMEEEADDGSKMGSDLVSQQDICIDSASSVRENKQPEGLELKQGKGEDSDVLSINADAYDSDIEGPCNEEAAAPEAPENTVQSEAGQIDDLEKDIEKSVNEILGLAESSPNEPKAATLAVPPPEDVQPSAQQLELLELEMRARAIKALMKAGDIKKPA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Design the protein with a signal peptide incorporated.2. The protein design must be focused on enhancing the efficiency of apoptotic process, negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway. The designed protein sequence is ",
    "response#1": "GLCIIINNKNFHENTGMACRSGTDVDAANLRETFMNLKYEVRIKNDLTCKEMLELMSNVSKEDHSKRSSFICVLLSHGEEGIIFGTNGPVNLKKLASFFRGDYCRSLTGKPKLFIIQACRGTELDCGIETDSGAEDDMACQKIPVEADFLYAYSTAPGYFSWRNAKNGSWFIQALCEMLKKHAHRLELMHILTRVNRKVAIEYESFSTDSAFHAKKQIPCIMSMLTKELN",
    "response#2": "MKMRLPTRSDMICGYACLKGNAAMRNTKRGSWYIEALTQVFSERACDMHVADMLVKVNALIKEREGYAPGTEFHRCKEMSEYCSTLCQQLYLFN",
    "response#3": "SVMCVEGETGSGKTTFLKRIAFLWASGCCPLLYRFQLVFYLSLSSITPDQGLANIICAQLLGAGGCISEVCLSSIIQQLQHQVLFLLDDYSGLASLPQALHTLITKNYLSRTCLLIAVHTNRVRGIRSYLDTSLEIKEFPLSNTVYILKKFFSHNIKRLLEFMVYFGQNEDLQGIHKTPLFVAAVCTDWFENPSDQPFQDMALFKSYMQYLSLKHKGAAKPLQATVSSCGQLALTGLFSSCFEFNSDDLAEAGVDEDEELTTCLMSKFTAQRLRPVYRFLGPLFQEFLAAMRLTA"
  },
  {
    "reference": "MILRDLLKEIEYSCLSGSEDVEIGTIVYDSRKIEKDCLFVCVKGANFDGHSAVNEAHEKGAAAVLVMDQVGSFDDMTVIKVQDTRYAMAKISAAWLSYPARKMKIIGITGTKGKTTTTYLIKSILEHAGYKVGLIGTIEIIIGDKHIPALNTTPESYVLQDYFKQMADIGTDIVVMEVSSQGLMMHRTAAIDFEIGIFTNIEPDHIGPNEHSSFEEYMACKSMLFRQCKLGIFNMDDKHVNDIMKDHTCQVVTYGLSDKASLKAENVEYTNKNGVLGASFKTKGLLEMQAFIDMPGIFSVYNGLCAIAVCMHFNIKPEDINTALLQAKVKGRIEMVEVPGDYTLMIDYAHNAMALESLLTTLKEYNPPRIVCLFGCGGNRSKLRRYEMGEVSGRLADLTVITSDNPRFEEPADIMEDIKTGIEKTSGQYVMIENRKDAIKYCIDNAKTGDVIVLAGKGHEDYQEIKGKKYPMDERVLVKEVLEGK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein have Mur ligase C-terminal domains with specific sequence motifs that are crucial for its activity.2. A protein with UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity functions is required to enable regulation of cell shape.3. The protein should be optimized for activity in Cell wall biogenesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.4. The designed protein should be able to compete with endogenous proteins or inhibitors for meso-2,6-diaminoheptanedioate binding. The designed protein sequence is ",
    "response#1": "VTGTSGKTTTTYLLEAGLMAEGHKVGLIGTTGTRIDGVAVPTKLTTPEAPKLQELFAMMLAEGCTHVVMEVSSHAISLGRIAGTRFAVGGFTNLSQDHLDFHNTMEEYFEAKAGFFRADSPLRAEKAVIMVDDEWGRTMSSVAGQQCLTVSASGAAADVQVGEVSTTLAGMQEFSLTIDGHSHHIELALPGQFNVANAALAVSALLGTGVPLSRIQEGLENLRGVPGRFDYIVSDSQITGIVDYAHTPDALENVLNTIQQIRNPNQKVITLVGCGGNRDAAKRPVMADIACRLSDKVILTSDNPRFEEPQAILEDMQKGVKPLDFKKTLSVLDRREAIKTACVLAEPNDIILVAGKGHETYQIIGTQKHPFDDRVVARRIIKEIGGA",
    "response#2": "TGTNGKTTTTFLIKQILENVGKKVGLIGTVQNMVGDEIYPAKYTTPDPYELQKLFSLMVKAGCEYCVMEVSSQALAQGRVNGLRFALGAFTNLTQDHLDYHKTWENYFASKRILFENCDIAVTNADDEHGLKIIDGLEFDKVVTYAVDTNDATYTAKNVKFKASGVEYEIVSNDSIGRCTCPIPGRFSVYNSLCATTGSGTYVDGVITGTNGKTTVATLLHRLARMFDRKAGLVSTVENRINDRVVPATHTTPDPVGLQALFADMLDAGCTHCFMEASSHALDQHRLSGSQLTGGVFTNITHDHLDYHGDFNAYISAKKSLFDKLPGVAFALTNADARHGDTMVQNTRARVVTYGTHQVADHKARIIENGLGGLQLHLDGQDLYTRLIGEFNASNLTAVYAVAIELGVPGEVVAKGLQTCAGVPGRLDIVGEKNGALAVVDYAHKPEALEAALAGLRPFATGRLICVFGCGGDRDRGKRPIMGAIATSNADIVIVTDDNPRTEDPAAIRAEILAAAPGAREIGDRAEAIRAAVALLQPGDVLVVAGKGHEDYQILGTEKIHFDDVEEAERALRNLG",
    "response#3": "VTGTNGKTSVSQLIAQACDLLGQRCGIVGTLGNGFYGALAEGRHTTPDALKLQATLAELAEGGASTVAMEVSSHGLAQGRVAALAFKVAVFTNLTRDHLDFHGTMEAYGEAKAPLFAWPALGTRVLNLDDAFGRELASRASSARTLTYSLSDPAASLYCQDPRFDDHGVRARLVTAQGSGELRSQLIGRFNLSNLLAAIATGASLGIASVEIIRALESFAGVSGRMQQVQAGKGDVHAIVDYAHTPDALAKVLETLRTVRSAGPATGANGEARILCVVGCGGDRDRTKRPVMARTAAELADLVILTSDNPRSEDPSAILGEMREGLDPIQMRKVTYNTDRAEAIRSACMQAQAGDVILVAGKGHETGQIVGDTVYPFDDVEQASVAVAALDGRVA"
  },
  {
    "reference": "MATIKDVARLAGVSTTTVSHVINKTRFVAEATQEKVMKAVDELNYAPSAVARSLKCNSTRTIGMLVTQSTNLFFSEVIDGVESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGILVMCSDLTEELKEMLDRHSDIPKVVMDWGPESSRADKIIDNSEEGGYLATKYLIDNGHTDIACLSGHFEKLACQERIAGFRRAMAEAKLPINEDWILEGNFECDTAVLVADKITAMEKRPTAVFCFNDTMALGLMSRLQQNGIKVPDDVSVIGYDNIELAEYFSPPLTTIHQPKRRVGKNAFEILLERIKDKEHEKRVFEMQPEIVIRNTVKKLN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein with DNA binding functions is required to enable purine nucleotide biosynthetic process, negative regulation of DNA-templated transcription.2. We require a protein that can specifically bind to the metabolites produced in the Purine metabolism pathway.3. The protein must exhibit the following characteristics: PurR is allosterically activated to bind its cognate DNA by binding the purine corepressors, hypoxanthine or guanine, thereby effecting transcription repression.; Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression.4. A protein with H-T-H motif, DNA binding capability for targeted gene regulation.5. The presence of HTH lacI-type domains is critical for the interaction of the designed protein with its intended targets.6. The designed protein should have a binding site that is specific to the target hypoxanthine ligand. The designed protein sequence is ",
    "response#1": "MATIKDVAKMAGVSTTTVSHVINKTRFVAKETEQQVLQAIKNLNYSPSAVARSLKVNTTKSIGMIVTTCENPRLLWDQLHRDARALAWRLIDLGPFNGIVAITRGGLIPAAIIARELEARLVETVSVVTYGRGAAGETEIGAPQVLKPPVAAGDGEGFLLIDDLVDTGTTARVVRSLLPKAHFATVYAKPAGKQLVDTFITEVSQNTWILFPWDTEPQTPFDLFSPC",
    "response#2": "MQSWEQFHRDARALSWRLSGDRGFEAIVCITRGGLVPALIIARELGIRLIDTVCIASYHDYKEQGAAQILKGVGPEVAALTADGGTRILLIDDLVDTGKTARLVRDMLPKAHFATVYAKPMGRPLVDTFVTEVSQDTWIYFPWDLGLSYVEPYRR",
    "response#3": "MPKHFIDNGHEKIGCLSGHSEKSTCRERLKGFNKAMAEAGITVNNNWIIDGDFECESAVEAANQYIAMKDRPTAIFCFNDIMAMALISTFEQAGVRVPDDISVIGYDNIDLAPYFSPPLTTIHQPKRRLGKTAIEILMERVKDKNHERRVFEMNPELVIRKSLNE"
  },
  {
    "reference": "MPDAHEASQVTPFLHHHWRIDMNSSATITVATEAISAGRMVLVVDDENRENEGDLIMAGEHISTEDVVFMLRYGSGIICTPMTGAIADDLDLPPMVPVNTDNHGTAFTVTVDAMSVGTGISAEDRALTIRALASSSTRAQSLRRPGHVFPLRARPGGVLQRNGHTEAAIDLMRLSGCREVAVITEVVGDNGVPLSGAALAEFTATHDIPMITIAALVDHRKRTEKVVTSSESANLPTAYGLFQARSYLSLADGIEHLVLIHGNIDTRDIRTEGVLARIHSECLTGDLIGSRRCDCGTQLEAALRQIVSDGAGVLIYLRGQEGRGIGLGRKLTAYALQEGGCDTVDANLQLGLPIDDRDYSVAAAVLRDLGIERIRLITNNPDKIASIDNGGLSVAERVQLPVTVTSDNLTYLRTKRDRMGHLLDLPHALPS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A key design requirement for the protein is the optimization of the Nucleophile; for GTP cyclohydrolase activity, Proton acceptor; for GTP cyclohydrolase activity to achieve high catalytic efficiency for GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.2. The designed protein should be optimized for zinc ion binding, GTP binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity activity in order to improve riboflavin biosynthetic process.3. The protein should be able to bind GTP ligand in a variety of conditions4. The designed protein have GTP cyclohydrolase II domains with specific sequence motifs that are crucial for its activity.5. The protein design should take into account the physiological conditions of the Cofactor biosynthesis pathway.6. The protein must bind to Zn(2+) in order to perform its enzymatic function.7. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.. The designed protein sequence is ",
    "response#1": "VRVDMPTEYGHFELVAFKEKNNKAEHLALIKGDIGNGEPVLTRVHSSCFTGDILGSLRCDCGDQLHAAMQMVEKEGRGLILYMNQEGRGIGLINKLRAYKLQEQGYDTVEANLELGFGVDERDYGVGAQILRYLGATKLRLMSNNPRKRAGLNGYGIEIVETVEPHDVYRVTEANMPTKYGDFKIVGYENKLNGEHHIALVKGNVSDGEPALVRVHSECLTGDAFGSLRCDCGEQYSAAMKKINDEGRGVLVYMRQEGRGIGLINKLKAYALQDKGMDTVEANLALGFPEDMRDYGIGAQILLDLGVQKIKLMTNNPKKLCGLSGYGIEIVDRVRAAEVVLPTPYGGDFKAIAYDNEVDNANHLALIKGEIDPEKPVLVRVHSECLTGDVFGSQRCDCGDQLHAAMQQIDREGAVVILYMRQEGRGIGLINKLKAYALQDEGHDTVEANEVLGFKADLRDYGIGAQILTELGVRQIRLMTNNPKKIVGLEGYGLKIVERVPSVPEGSLVRAAEVVLPTPYGGDFKAIAYDNEVDNANHLALIKGEIDPEKPVLVRVHSECLTGDVFGSQRCDCGDQLHAAMQQIDREGAVVILYMRQEGRGIGLINKLKAYALQDEGHDTVEANEVLGFKADLRDYGIGAQILTELGVRQIRLMTNNPKKIVGLEGYGLKIVERVPSVPEGSLVKEVV",
    "response#2": "TSAMPTEFGDFRATAYRNTLDGTEHLALVMGDLAAASRTTEGALVRVHSECLTGDILGSLRCDCGGQLEQALAAIAAEGAGAVVYLRGHEGRGIGLGHKIRAYALQEQGLDTVDANTAQGLPVDSRSYGVGAQILGDLGATRIRLITNNPAKYGGLDGYGLTITGRADVAQAVVAALERVSSCRLPTKYGVFELHGYRELSNGQEHLALVMGQPGEDASPLVRVHSECLAGEVFGSLRCDCAPQLSQSMSLVAAAGCGAIVYLRGHEGRGIGLLHKLRAYALQDGGADTVQANLALGFPADQRDYAIGAQILRQIGVSRMRLMTNNPRKYEGLADFGLEIAERVAG",
    "response#3": "MKNREVEIKLPTEYGAFRAVGYTSIIDQKEHVAIIKGDISGAEPVLVRVHSECLTGDVFGSNRCDCGPQLEAALTQIEAEGRGVLLYMRQEGRGIGLINKLKAYKLQEEGYDTVEANNKLGFAADLRDYGIGAQILRELGITKMRLLTNNPRKIAGLNGYGLEVIERVPHPEVPEGRAAEVVLPTPYGGDFKAIAYDNEVDNANHLALIKGEIDPEKPVLVRVHSECLTGDVFGSQRCDCGDQLHAAMQQIDREGAVVILYMRQEGRGIGLINKLKAYALQDEGHDTVEANEVLGFKADLRDYGIGAQILTELGVRQIRLMTNNPKKIVGLEGYGLKIVERVPRHDIPEEVTHVVDIPTQFGNFTFAGYSDEIEHKEYIAVMKGEIKNKENVTVRLHSECLTGDVFGSRRCDCQEQLHRALHELEESGEGLIIYLRQEGRGIGILNKLKAYKLQDEGYDTVEANHKLGFSDDLRDYAVAAQIIKDLGIKSIILKTNNPKKIEGLEKYGVKVAGRVRVDMPTEYGHFELVAFKEKNNKAEHLALIKGDIGNGEPVLTRVHSSCFTGDILGSLRCDCGDQLHAAMQMVEKEGRGLILYMNQEGRGIGLINKLRAYKLQEQGYDTVEANLELGFGVDERDYGVGAQILRYLGATKLRLMSNNPRKRAGLNGYGIEIVETVPKREIPEEVSEEVGEEIEYREVKLEEVGLRRAKLRRVIVTPDVWAKMILNRAIKGDPIDVAITAGIDPIEIDTLEV"
  },
  {
    "reference": "MVDLSFNATVFMQMFHFLLMLVVLRLFAYRPLMNVIEQRQAYIADEIEAAEKQKAAAAELRSQLEADLAKAREEAKAIVARATKASDEQAQAIMEQARTEAQRLKEEALAEIGREREKAIAQLKDEVASLAVLVAAKVVKDGLTIDAQHNLVQNAIKEVGQLQ",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0).2. The designed protein localizes to the plasma membrane. The designed protein sequence is ",
    "response#1": "MP",
    "response#2": "MPIGVPKVPFRSPGESDAAW",
    "response#3": "LILLPG"
  },
  {
    "reference": "MTTQQVVLASGNAGKLRELGRILTPLGMELVSQGEFDMPQVPEDGLSFVENAIIKARAAAAHCGLPAIADDSGLEVDYLQGAPGIHSARYSGQGDEANNAKLLAALEGVDEAQRSARFQCVLVYMRHANDPTPLVCQGSWEGRILTETRGVEGFGYDPLFYVPEHDCSSAELPRDLKNAISHRAKASALLLDALR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be able to perform ITP diphosphatase activity under specific conditions.2. For general function, the protein need meet that Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions. The designed protein sequence is ",
    "response#1": "MI",
    "response#2": "MN",
    "response#3": "MSFLGL"
  },
  {
    "reference": "MSDRNETTGEGRTGAIVLAAGQGTRMRASGPKVLQPLLGKPLIEHVLDAIAAAGVDGPAVVVVPADDDRVAHALQDRPGVRVAVQPQPDGTGGAALAGLRALEATGLPPRVLVACGDAPLIRPETFAALIERHEQAAAAATVLTAEVPDPSGYGRIVRSGDGERVARIVEDREARPGERGIHEINSGTFVFDTEGLADLLEQLPVQPYKGERYLTDVVQRLVAARKTVAAFRAEQWQEVLGVNTIEQLADAGAILRKRIVYGLMRSGVQIPAPELVWIEVDVEVAPGARIEPFSVLRRGARVGADAVVGPFAHLGPGAQLAARAEVGNFVEVKRSTIGEGAKAKHLAYIGDAQIGPRANVGAGTVFCNYDGKDKHRSVVGEGAFVGSGSLLVAPCELAPGARTGAGAVVTRGSRVESGETWVGVPARPLDPRR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein that can modulate the overall flux of metabolites through the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D- glucosamine biosynthesis pathway, enhancing or suppressing its activity as needed.2. A protein that can perform glucosamine-1-phosphate N-acetyltransferase activity, magnesium ion binding, UDP-N-acetylglucosamine diphosphorylase activity in cytoplasm to support lipid A biosynthetic process.3. The protein must exhibit the following characteristics: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).4. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate.5. The designed protein have Nucleotidyl transferase domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "MGYAVILAAGQGTRMKSSLYKVLHPVCGKPMVQHVVDQVSKLELSKTVTVVGHGAEKVKSQLGDTTEYALQSEQLGTAHAVMQASSFLENEIGTTIVICGDTPLITAETMSSLLAHHQETNAMATILTAKADDPTGYGRIVRNSEGTVEKIVEHKDANASEREITEINTGTYCFDNQELFRALSNVSNDNVQGEYYLPDVIEILQKEGKIDGAEEVQGINDRVQLARAEKVMRDRINKAHMLNGVTIVDPANTYIEADVKIGADTVIEPGVFLRGKTVIGENCKIGPQADLKDVRVESGTRIRYAVIEDSVIGEEAKVGPYVLIRPETELGNKTKVGCFIDLKKAKISDGTKISHLAYVGDAEVGRKVNIGCGVITVNYDGVNKHKTVIKDEAFVGCNVNLVAPVTVEQGAYVAAGSTITKDVPMKALAIARERQINKQGWVDKKGLLKK",
    "response#2": "TVILAAGKGTRMKSKLPKVLHKAAGKSMVQHVIDAAKAAGAKRNIVVTGFGGDVVREAIGAQAEFVEQKEQLGTGHAVMQTADLLQDETGTVMVLCGDTPLLTGDLLKKLFDSHVEAQAKATVLTAIMPDATGYGRIIRMADGSVQKIVEHKDATEEERQVKEVNSGIYCFDAKALFESLKKVTNDNAQGEYYLPDVLEILQKQGEKIWAVAADGVTIADPATTWIEATVELEPDTVVHPYTTLRGRTRVAAGAEVGPHVVADDAEIGPRATVGPFAYLRPGATLAADSKVGAFVEVKNARIGERTKVPHLSYIGDAEIGEDTNIGAGAITANYRPERHAGKQRTVIGRNVHTGSDNVFVAPVEIGDDAWIAAGSTITEDVPPGALAVARARDIWQIEGVNDRVQLAAMARELNRRILDRWMREGVSLIDPSVFYCDDDVVIEADAVIYPNVFLQKGTVIRKGAVVYPGCRIKNSEIGENCEIKDNCLITDSYVGAKSAIGPMAQLRPGTVLKGKNKIGNFVETKKAEMGIGSKASHLTYLGDAEIGKDVNIGCGTITCNYDGISKYKTVIGDGVFVGSDVQLVAPVTVGEGALIAAGSTITKDVPADALGITRASGITVREWSKK",
    "response#3": "VIILAAGKGSRMKSRLPKVLHQIAGKSLVQHVIDSCRSMGANHCHLVVGHGAEAVKQAVDGGRVELSFVEQKQQLGTGHAVKVALDGLKPDTPTLILYGDVPLIHHDTLSELVAIHESDKSGIAILTAKVEDPTGYGRIIRNAGGRVQAIVEEKDADEGQKKIKEINTGILVAPTEHLRKWLASLSNSNAQGEYYMTDVIALAYQEGVEIQTVQPEQEREILGVNSKVQLAELERVYQRNAAERLMLEGVTLMDPDTTYVDADVKVGRDTVIYPMTWLEGDTVIGEECEIGPSIRFQNVKAGNRVTGQFTYAHDCELEDGVILGQFTHIRPDTHLAEGVKIGNFVEVKNSTVGKGSKLPHLSYIGDTDMGSGCNMGCGTITVNYDGKTKFRTKIGNDAFIGCNSNLVAPVEVEDGAYVGAGSTITKKVPSNTLAIARARQKNIEGWKRPVKK"
  },
  {
    "reference": "MSRKPSGLGRGLEALLPKTGAGVVRLPLASIRPNPRQPRKRFAEESLKELADSIREKGLLQPLLVRPQGDGYELVAGERRYRAALMAGLQEVPAVVKDLTDREALELALVENLQREDLSPVEEARGYQALLEMGLTQEEVARRVGKARSTVANALRLLQLPPEALEALERGEITAGHARALLMLEPEDRLWGLKEILEKGLSVRQAEALRERLAMAPKRSAEPSPLSLELSRHLGLPVRVVGGKKGKVVIQYRSLEELEALLRRLGYQA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Probably involved in chromosome partitioning; Stimulates the ATPase activity 10- fold of Soj; the first 20 residues may be responsible.; Binds to a plasmid centromere-like site parS2. The target protein must exhibit strand-helix-helix-strand-strand-helix-strand-helix-helix-helix-helix-helix-helix-helix-helix as its primary conformation.3. The designed protein should contain at least one H-T-H motif domain that can specifically recognize and bind to DNA.4. A protein with DNA binding functions is required to enable chromosome segregation. The designed protein sequence is ",
    "response#1": "MLSTGTKSLDSLLGGGFAPGVLTQVYGPYASGKTTLALQTGLLSGKKVAYVDTEGGFSPERLVQMAETRGLNPEEALSRFILFTPSDFKEQRRVIGSLKKTVDSNFALVVVDSITAHYRAEENRSGLIAELSRQLQVLLWIARKHNIPVIVINQA",
    "response#2": "MLSTGTKSLDSLLGGGFAPGVLTQVYGPYASGKTTLALQTGLLSGKKVAYVDTEGGFSPERLVQMAETRGLNPEEALSRFILFTPSDFKEQRRVIGSLKKTVDSNFALVVVDSITAHYRAEENRSGLIAELSRQLQVLLWIARKHNIPVIVINQL",
    "response#3": "MLSTGTKSLDSLLGGGFAPGVLTQVYGPYASGKTTLALQTGLLSGKKVAYVDTEGGFSPERLVQMAETRGLNPEEALSRFILFTPSDFKEQRRVIGSLKKTVDSNFALVVVDSITAHYRAEENRSGLIAELSRQLQVLLWIARKHNIPVIVINQQ"
  },
  {
    "reference": "MAKDLNDSGFPPKRKPLLRPQRSDFTANSSTTMNVNANTRGRGRQKQEGGKGSSRSPSLHSPKSWIRSASATGILGLRRPELAHSHSHAPSTGTPAGGNRSPLRRSTANATPVETGRSLTDGDINNVVDVLPSFEMYNTLHRHIPQGNVDPDRHDFPPSYQEANNSTATGAAGSSADLSHQSLSTDALGATRSSSTSNLENLIPLRTEHHSIAAHQSTAVDEDSLDIPPILDDLNDTDNIFIDKLYTLPKMSTPIEITIKTTKHAPIPHVKPEEESILKEYTSGDLIHGFITIENKSQANLKFEMFYVTLESYISIIDKVKSKRTIKRFLRMVDLSASWSYSKIALGSGVDFIPADVDYDGSVFGLNNSRVLEPGVKYKKFFIFKLPLQLLDVTCKQEHFSHCLLPPSFGIDKYRNNCKYSGIKVNRVLGCGHLGTKGSPILTNDMSDDNLSINYTIDARIVGKDQKASKLYIMKEREYNLRVIPFGFDANVVGERTTMSQLNDITKLVQERLDALRKIFQRLEKKEPITNRDIHGADLSGTIDDSIESDSQEILQRKLDQLHIKNRNNYLVNYNDLKLGHDLDNGRSGNSGHNTDTSRAWGPFVESELKYKLKNKSNSSSFLNFSHFLNSSSSSMSSSSNAGKNNHDLTGNKERTGLILVKAKIPKQGLPYWAPSLLRKTNVFESKSKHDQENWVRLSELIPEDVKKPLEKLDLQLTCIESDNSLPHDPPEIQSITTELICITAKSDNSIPIKLNSELLMNKEKLTS",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must be able to regulate protein polyubiquitination.2. The protein design should take into account the physiological conditions of the protein ubiquitination pathway. The designed protein sequence is ",
    "response#1": "PTTFKDLLNNFIKTTESNIMKQTICSYLDCERSCEADILKNTSYKGFFQLMCSKSCCVYFHKICWKKFKNLKYPGENDQSFSGKKCLKEGCTGDMVRMLQCDVPR",
    "response#2": "PHNIDHCLTWARSEFEGLLEKTPAEVNAYLSDPVEYMKAMRTAGDAQARDTLGRVVECLEKEKCNSFQDCITWARLRFEDYFANRVKQLCYTFPEDAATSTGAPFWSAPFV",
    "response#3": "PHNIDHCLTWARSEFEGLLEKTPAEVNAYLSDPVEYMKAMRTAGDAQARDTLGRVVECLEKEKCNSFQDCITWARLRFEDYFANRVKQLCYTFPEDAATSTGAPFWSAPL"
  },
  {
    "reference": "MPSVAVPKISREEGPIGINAILLGPPGSGKGTQAPKLKEKYCVCHLSTGDMLRAEIQSGSVLGAKLKKIMDEGKLVSDDLVVNMIDSNLDKPECKNGFLLDGFPRTVVQAEKLDDLLEKRDTSLDAVIEFGIDDSLLVRRITGRLIHPKSGRSYHEEFHPPRKPMTDDVTGEPLVRRSDDTAEALKKRLQAYHTQTRPLVDYYQIRGIHHRIDASKAANEVFSKIDSIFMRARKSRIGF",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that can perform adenylate kinase activity in mitochondrion to support AMP metabolic process.2. The presence of Adenylate kinase active site lid domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "GFEPHPALMNAVFLLACHFARSPYCTEIEPVFFSRTLHEITVALDNSDRLVDVVQASCLLAVYLYINCRSLEGYCHSFSAARLAVGLGMHQIKRFLPLDGMGGASTEDITPIPIPPPRDRAEVQDRISAFWQVFMVDRCWSVASGLPVALPS",
    "response#2": "GFEPHPALMNAVFLLACHFARSPYCTEIEPVFFSRTLHEITVALDNSDRLVDVVQASCLLAVYLYINCRSLEGYCHSFSAARLAVGLGMHQIKRFLPLDGMGGASTEDITPIPIPPPRDRAEVQDRISAFWQVFMVDRCWSVASGLPVALPF",
    "response#3": "GFEPHPALMNAVFLLACHFARSPYCTEIEPVFFSRTLHEITVALDNSDRLVDVVQASCLLAVYLYINCRSLEGYCHSFSAARLAVGLGMHQIKRFLPLDGMGGASTEDITPIPIPPPRDRAEVQDRISAFWQVFMVDRCWSVASGLPVALPR"
  },
  {
    "reference": "MNRLQDDYDPYAVEEPSDEEPALSSSEDELDVLLHGTPDQKRKLIRECLTGESESSSEDEFEKEMEAELNSTMKTMEDQLSSLGTGSSSGVAKVGGVTEKFYDEIYFDSDSEDEDKTVTKKKKKKQHRIPTNDELLYDPEKDNRDQAWVDAKRRGYHAFGLQRPRQKQQPVPNSDAVLNCPACMTTLCLDCQRHESYKTQYRAMFVMNCSINREEVLRYKNPENRRKRRSAKKMRSNPEDPAEREAEEIYHPVMCTECSTEVAVYDKDEVFHFFNVLASHS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Promotes S-phase entry, and inhibits p14(ARP) expression ..2. The designed protein's functionality will benefit from Acidic residues compositional bias in its structure.3. The designed protein localizes to the cytoplasm, nucleus. The designed protein sequence is ",
    "response#1": "FTFKWPKGPEAIILTGTFDDWKGTLPMVKDPSGAFEITLPVTFDSPSSKFYFKFIVDGQWLPSKDYKVNIDEGVENNFID",
    "response#2": "FTFKWPKGPEAIILTGTFDDWKGTLPMVKDPSGAFEITLPVTFDSPSSKFYFKFIVDGQWLPSKDYKVNIDEGVENNFIP",
    "response#3": "FTFKWPKGPEAIILTGTFDDWKGTLPMVKDPSGAFEITLPVTFDSPSSKFYFKFIVDGQWLPSKDYKVNIDEGVENNFIK"
  },
  {
    "reference": "MEIKILSERYNPLLKRKEYRFIVDHDGPTPTFKDVKLKLAAILNANKDLLIVEKIVEEAGMQRARGYAKLYDNEEMLKLVEREHILRKNKIEEETAAEEGE",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must be able to structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "PKILITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSLQKGTFL",
    "response#2": "PKILITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSLQKGTFR",
    "response#3": "LITTSDRPRGRSVRFTEQLSSIIPNSDVYYRRGLALKKIIPQCVSRDYTDLLVINEDRKVPNGLVLCHLPDGPTAHFKISNVRLRKEMKRKGKEPTEHKPEVILNNFTTRLGHSIGRMFASLYPHDPHFVGRQVATFHNQRDYIFFRYHRYLFKSEKKVGIQELGPRFTLKLRSC"
  },
  {
    "reference": "MGGKWSKRMAGWPTIRERIRRTDPAEPAAEGVGAVSRDLEKYGAITSSNTVETNPDCAWLEAQQEDEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLDGLIWSQKRQDILDLWVYNTQGYFPDWHNYTPGPGPRYPLTFGWCFELIPVDPKEVEEAAEGENNCLLHPINQHGMEDPEREVLVWKFNSRLAFEHMARMKHPEFYKNC",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should be expressed specifically in the host cell Golgi membrane2. The protein must exhibit the following characteristics: Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Protects the infected cells from apoptosis in order to keep them alive until the next virus generation is ready to strike.3. The designed protein should contain at least one Dileucine internalization motif; necessary for CD4 internalization, Diacidic; necessary for CD4 internalization, PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization motif to achieve its intended function. The designed protein sequence is ",
    "response#1": "MGNKWSKSWPAVRERIRRTEPAXE",
    "response#2": "MGGKWSKSSLVGWPAVRERL",
    "response#3": "MGGKWSKSSLVGWPAVRERM"
  },
  {
    "reference": "MSKNNLNETESKIEIEAKVVELLSNDKFKVELPNKKTIIAYVSGKIRINNIRILPGDKVRIELSPYDPTRARITYRFK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds; One of the essential components for the initiation of protein synthesis; Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC).2. The designed protein must be optimized for ribosome binding, rRNA binding. The designed protein sequence is ",
    "response#1": "MAKEDCIEMEGVVLEALPNTMFRVELENGRIVTAHISGKMRKNYIRILTGDKVVVEITPYDLTKGRIKFRSK",
    "response#2": "MTKEESIEIQGIVLDTLANAVFRVELENGHIVTAHISGKMRKNYIRILTGDKVTVELTPYDLKKGRITFRSK",
    "response#3": "MKNDKLFLTGKILEIIHGDKYRVMLENNVEVDAHLAGKMKMKRTKILPGDVVEVEFSPYDLKLGRITQRIH"
  },
  {
    "reference": "MKSLSKLVRTVDESQIIEFFQESVGEWCSQRRYYTLPDGETKEMMSMITIRFLEQGCDELQKLAQIHKLAESVFLICGAEVTWCSTDVLKNRSESEGSTLFGALGNILYRDRGFATSKPVTAQYNFPNPKTLCLRTEYNGSVFEEELKLIGSKYRTRQTIISRAGEQLMIGQYIEKRIVQ",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must possess lyase activity to support protein-phycocyanobilin linkage under certain conditions.2. The protein must exhibit the following characteristics: Covalently attaches a chromophore to Cys residue(s) of phycobiliproteins (Potential); In vitro does not act as a chromophore lyase for ApcA1, ApcA2, ApcB, ApcD, ApcF, CpcB or PecB, the lyase activity is therefore unsure.. The designed protein sequence is ",
    "response#1": "DLQGLAIPDIYLVAADKSLRYVMKPNLSNTEKAQVIKACYRQVFERDIAKAYGLSLLELESKLKNLQISVKEFIRALGKSTLYRKNFYEGFTNSRVVELAFRHFMGRGLSSLQEFRKYFAILSSNGLDALIDSIINNSEYAEYFGEETVPYIRGYGQEAQECRNWGSQFALFKYSAPFRTL",
    "response#2": "DLQGLAIPDIYLVAADKSLRYVMKPNLSNTEKAQVIKACYRQVFERDIAKAYGLSLLELESKLKNLQISVKEFIRALGKSTLYRKNFYEGFTNSRVVELAFRHFMGRGLSSLQEFRKYFAILSSNGLDALIDSIINNSEYAEYFGEETVPYIRGYGQEAQECRNWGSQFALFKYSAPFRTL",
    "response#3": "DLQGLAIPDIYLVAADKSLRYVMKPNLSNTEKAQVIKACYRQVFERDIAKAYGLSLLELESKLKNLQISVKEFIRALGKSTLYRKNFYEGFTNSRVVELAFRHFMGRGLSSLQEFRKYFAILSSNGLDALIDSIINNSEYAEYFGEETVPYIRGYGQEAQECRNWGSQFALFKYSAPFRTV"
  },
  {
    "reference": "MRKLFGTDGIRGVANVHPMTMEIAMQVGRAIAFLVKKENYRHRIVIGKDTRLSGYMIENAIVAGICSMGVDVLLVGPLPTPGIAFITTSMRADAGVVISASHNPFQDNGIKIFFSDGFKLPDAMELKIEDLVLSQRMLALQPLAEEVGRASRIDDAKGRYIVFLKNTFPKKYTLDGFHIVIDCAHGATYGVAPHVFEELGAKVTALGIEPNGQNINAGCGALHPELMAGKVKELGADIGLAFDGDGDRLIVCDEHGVVVDGDHVMAICAKELLAQRKSKKKTLVATVMSNMGLEVAMKKMGGHLVRADVGDRYVVECMRKNGYSFGGEQSGHLVFLEHMTTGDGILAALQILAIMKKRKKTLSELAQVMQSFPQVLKNVRTAKKISVDSIVGFADAVKKYEMQLGDTGRILVRPSGTEPVIRVMVEGLDSAEINDIADELCELIRRVSNS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should have phosphoglucosamine mutase activity, magnesium ion binding to facilitate carbohydrate metabolic process.2. The protein should have a high affinity for Mg(2+).3. The designed protein must have a Phosphoserine intermediate active site that is highly conserved among related enzymes.4. The protein requires Mg(2+) to activate a critical catalytic site.5. For general function, the protein need meet that Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate. The designed protein sequence is ",
    "response#1": "NGDELLYIMVKDRMRVRPIEGAVGTLMTNMALEVAFKEMGVGFARANVGDRYVLEVLRERGWQVGGEGSGHMLCLDKHTTGDGIVSALQILSALKRSGLSLAELTQA",
    "response#2": "DGDQIMAILAVAMKEAGELADDTLVTTVMSNLGLHLAMREHGVKLRTTAVGDRYVLAELREGGFSLGGEQSGHVVLPDHATTGDGLLTALRLMGRVVETGRSLAELAATML",
    "response#3": "DGDQIMAILAVAMKEAGELADDTLVTTVMSNLGLHLAMREHGVKLRTTAVGDRYVLAELREGGFSLGGEQSGHVVLPDHATTGDGLLTALRLMGRVVETGRSLAELAATMVL"
  },
  {
    "reference": "MAEDDPYLGGHEQMFPLNPLNHTIFNPELFQPEMALPTADGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYMGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVNAGPKDMVVGFANLGILHVTKKKVFETLEARMIDACIRGYNPGLLVHPDLAYLQAEGGGDRQLTDREKELVRQAALQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEESGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGGAGSTGPGYGFPNYGFPTYGGITFHPGATKSNAGMKHGTLDTESKNDPEGCDKSDDRETVNLSGKVAGTTEQDKASGKPSSGDAEGVQDENSRFQDNLFLEKAMQLAKRHASALFDYAVTGDVKMLLAVQRHLAAAQDENGDSVLHLAIIHLHAQLVRDLLEVTSGLISDEIINMRNDLYQTPLHLAVLTKQEEVVEDLLRAGADLSLLDRLGNSVLHLAAKEGHETVLSILLKHKKAALLINHPNGEGLSAIHLAMVSNSLPCLLLLLAAGADVNAQEQKSGRTALHLAVEHDNISLAGCLLLEGDAHVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLLENCEPLYDLEDSWDSAEEDEGVVPGTTP",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein contains novel RHD domains that confer a unique function or activity.2. The designed protein should have RNA polymerase II cis-regulatory region sequence-specific DNA binding, identical protein binding, actinin binding, DNA-binding transcription repressor activity, RNA polymerase II-specific within NF-kappaB complex, nucleoplasm, cytosol, mitochondrion to regulate JNK cascade, positive regulation of hyaluronan biosynthetic process.3. The protein should exhibit Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "PYLQIIEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSFPQVKICNYVGPAKVIVQLVTNGKYVHLHAHSLVGKFCEDGVCTVNAGPKDMVVGFANLGILHVTKKKVFETLETRMIDACKKGYNPGLLVHPELGHLQAEGCGERQLTEREREIIRQAAVQQTKEMDLSVVRLMFTAFLPDSNGSFTRRLDPVISDAIYDSC",
    "response#2": "PSLPALDWQLPSHSGPYELRIEVQPKSHHRAHYETEGSRGAVKASAGGHPIVQLHGYLENEPLTLQLFIGTADDRLLRPHAFYQVHRITGKTVSTTSHEIILSNTKVLEIPLLPENNMRAIIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHIPQPNGRTLSLQVASNPIECSQRSC",
    "response#3": "STLPPLDWPLPSQYEQYELKIEVQPRTHHRAHYETEGSRGAVKAAPGGHPVVKLLGYDEKPLTLQMFIGTADERNLRPHAFYQVHRITGKMVTTASYESIISGTKVLEMSLLPENNMAANIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHVPQGNGKVVSVQAASVPIECSQRSC"
  },
  {
    "reference": "MSVFDLFRGFFGFPGPRSHRDPFFGGMTRDDDDDDDDDDEAEEDRGAWGRESYAFDGSQPPEEFGFSFSPRGGMRFHGNFGFDDLVRDFNSIFSEMGAWTLPSHSPELPGPESETPGERLREGQTLRDSMLKYPDSHQPRIFEGVLESHAKPESPKPAPDWGSQGPFHRLDDTWPVSPHSRAKEDKDLDSQVSQEGLGPLLQPQPKSYFKSISVTKITKPDGTVEERRTVVDSEGRRETTVTHQEAHDSSRSDPDSQRSSALDDPFSILDLLLGRWFRSR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Slows down the rate of inactivation of KCNC3 channels; May be involved in internalization of ABC transporters such as ABCB11. The designed protein sequence is ",
    "response#1": "LIFSKHAVITLRHGRLCFMLRVGDLRKSMIISATIHMQVVRKTTSPEGEVVPLHQVDIPMENGVGGNSIFLVAPLIIYHVIDSNSPLYDLAPSDLHHHQDLEIIVILEGVVETTGITTQARTSYLADEILWGQRFVPIVAEEDGRYSVDYSKFGNTVKVPTPLCTARQLDEDC",
    "response#2": "KGQLLAIAGSTGAGKTSLLMMIMGELEPSAGKIKHSGRISFSPQVSWIMPGTIKENIVFGVSYDQYRYLSVIKACQLEEDISKFPEKDNTVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFSYLDLFTEKEIFESCVCKLMANKTRILVTSKVEQLKKADKVLILHEGSCYFYGTFSELEDQRPEFSSHLIGFDHFNAERRNSIITETLRRCSIDSDPSAVRNEVKNKSFKQVADFTEKRKSSIINPRKSSRKFSLMQKSQPQMSGIEEEDMPAEQGERKLSLVPESEQGEASLPRSNFLNTGPTFQGRRRQSVLNLMTRTSISQGSNAFATRNASVRKMSVNSYSNSSFDLDIYNRRLI",
    "response#3": "EFSSHLIGFDHFNAERRNSIITETLRRCSIDSDPSAVRNEVKNKSFKQVADFTEKRKSSIINPRKSSRKFSLMQKSQPQMSGIEEEDMPAEQGERKLSLVPESEQGEASLPRSNFLNTGPTFQGRRRQSVLNLMTRTSISQGSNAFATRNASVRKMSVNSYSNSSFDLDIYNRRLSQDSILEVSEEINEEDLKECFLDDTDSQSPTTTWNTC"
  },
  {
    "reference": "MTLAPTDVWSALSKVWPGASFEQPLPELPASLRLTTDSRALEVGDIFVAVPGSQRDGRDFIRAVLEQGASLVLAHTTERHATWDGRVLYLPHLAARLGELGKQLFNVPDDLEVIGVTGTNGKSSVTHYIAELSEALGVPAGVVGTLGVGRPGQLVDTGLTTPGPLALQAAFGELALQGIKRVALEASSHALDQHRLDAANIKVGVFTNLTRDHLDYHGSMAAYAAAKAKLFRRSELELAVVNADDPLARLMLAGCDQSVRVLAVGQDEAVTLRVMDVQTSETGQQALIATPEGERMLVLGLVGRFNLDNVLLAMATLYGWGHSINSLFEAAAALTPVPGRMALYHAKGVPGVVIDYAHTPDALHNALTALRAHLSKAGKLWCVFGCGGERDTGKRAEMAKAAEALADVVVVTDDNPRGESAAQIRQHIIAGLASTATLLEIADRRHALSQVIQQAAQNDVILVAGKGHEAYQEINGVRHAYNDEDEVMKAFALRGSAQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. The designed protein must have at least one Mur ligase central domain.3. The protein's functional design necessitates the inclusion of a Meso-diaminopimelate recognition motif motif.4. The protein should have a high affinity for ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate.5. The protein design should take into account the physiological conditions of the Cell wall biogenesis pathway.6. A protein that can perform magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, ATP binding in cytoplasm to support regulation of cell shape, cell division, peptidoglycan biosynthetic process, cell wall organization. The designed protein sequence is ",
    "response#1": "MKLRDLAEAAAGVRVTGRGDVRVVVDSREAGPGALFVARRGVHDDGHRFVEAALAAGAVAALVAPGGWGVTGTNGKTTVSCLVRELMEYKGRRTGLIGTIEHILGENRIIDSFTTPDAVLLQKYFAEMVKQNLSTAVTEVSSIGLSLGRVRETKFLAGVLTNISLDHLDFHGSFEEYVAAKQQLFAALPEQGIAVVNLDCQYAQSFLASSPARGVSYAVHQEADYRAINLRFSSSGSSCDIWYQGEVFPCETSLVGEHNVYNILAALAVATALGVPLAACMEGLASVVPPPGRLDPVLSGPCPVYIDYAHTPDALDNVCKILSQLLPKDGRLIIVFGCGGDRDRSKRPLMAKVSERYGFSVVTSDNPRTEDPERIIADICSGFSTDCYVVEGDRKLAIIKAMSMASDKDIVLVAGKGHETYQIIGTERRPFDDRAIAAAVLARRKGAA",
    "response#2": "MKLKDLLYKVTILSVSGNTNVAITGLNNDSRSVQPGTLFFCLPGYKVDGHDFAAAAASNGAAALVVERKLDVPESISYLEVVDSRRSLSLLSANYYGNPAKKLKLIGVTGTNGKTTTTYLIERILSDAGKKVGLIGTIANYIGDEKLPAERTTPESLDLQDLFAEMKENGCEYCVMEVSSHALELDRVYGCDFEVGIFTNLTQDHLDFHKTFENYFKAKKKLFSRTKIAVVNIDDDYGQRIIKETPAETLTYGIEKAADLRAENIKMTLKGTDFELITPLGSFKIKSPLVGKHNVYNILAAISASLALGINLETIKEGIENTEAVPGRFEVVRAANGTTAVVDYAHTPDALENVLRTIEEIRTPDQQLIVVCGCGGDRDRTKRPEMARIAVKYATTAIFTSDNPRHESPEAILDEMTAGLDAGTRCVRITDRAEAIRTAVLLSRPGDILLVAGKGHETYQILKDKTIHFDDREVVREILG",
    "response#3": "MKLKELAALLHCEVVGDDTAVTGISMDSRKVSEGDLFVCIKGLQHDSHLYAADAEANGAVAIIAERPLASTLPVIVVDDSRTALAQLADAFFGITGTDGKTTTSFLLRSVLQSAGYRTGLIGTVITSSGEEAVPSLYTTPESLELQEILAHMSLEGNEYVVMEVSSHALALDRVFGIPFEMGIFTNLSRDHLDFHGDFQHYRDVKALLFERLRGQDSCAVINLDDPNAQFIRDKTKVPVVTFSVEMEADVFLLNANLTMEMTEIEASTPQGPIHFQLHLLGRYNVSNALASVAVTGTNGKTTVSCLVRELMEYKGRRTGLIGTIEHILGENRIIDSFTTPDAVLLQKYFAEMVKQNLSTAVTEVSSIGLSLGRVRETKFLAGVLTNISLDHLDFHGSFEEYVAAKQQLFAALPEQGIAVVNLDCQYAQSFLASSPARGVSYAVHQEADYRAINLRFSSSGSSCDIWYQGEVFPCETSLVGEHNVYNILAALAVCHRLGVSFQDIAKGLAEAEGIPGRMELVGKTKTGASVIVDYAHKPLALETVLKSLRAHVQGQIIVIFGCGGNRDQAKRKMMGEIAKDYADSIIVTDDNPRFEDPQAIRKQILEGVPEAYEIASREEAIAFALHKAEAGDICLIAGKGHEDYQIIGHTKRHFDDREELRRALSARG"
  },
  {
    "reference": "MSKFIFVTGGVVSSLGKGIAVASIGRMLKSRDLSISVLKLDPYLNVDPGTMSPYQHGEVFVTVDGSETDLDLGHYERFIDVELTELSNVTAGQIYSEVISNERAGKYLGGTIQTVPHVTNLIKERILKLADKSQADVVVVEVGGTVGDIEGQPFVEAIRQVRNTVGQANTFYVHVTLLPYLGATGELKTKPTQHSVNTLRGMGVQPDAILCRADQETGDDERKKIALYCDVKPEAVISLPTLTTVYEVPLHLENEGLGDILINGLKLPDTAPDLKSWQTIVSVDREENPETEIALVGKYVELQDSYLSVVEALRHAGLHNQRKVKINWIAAEDIEQQGPDQLLSSACGIIIPGGFGKRGLEGMITAANYARKSNIPYLGLCLGMQIMVVEYARNELGITDATSLEIDPKAANPVISFMPGQKELHSTGGTMRLGVYPCAFKDGTKVRQAYGREHVEERHRHRYEFNNDYRLQLEEAGLVASGTSPDGSLVEVTEVYSHPFMVGSQFHPEFASRPERPHPLFKEFIRISKSVLREGGQHDLFQDQD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates..2. The designed protein should have a high specificity for ATP, Mg(2+), CTP, L-glutamine binding, with minimal off-target effects.3. The designed protein should contain one or more CTP synthase N-terminal domains that are essential for its function.4. Ideal protein should have a binding affinity for key enzymes in the CTP biosynthesis via de novo pathway pathway to enhance the overall efficiency of the pathway.5. The Nucleophile active site of the designed protein must be optimized for substrate binding and specificity.6. The designed protein must be able to regulate 'de novo' CTP biosynthetic process. The designed protein sequence is ",
    "response#1": "KFIFVTGGVVSSLGKGITAASLGRLLKSRGLKVAIQKLDPYINIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDASLGKSSNVTTGGIYWSVISKERKGDYLGATVQVIPHITNEIKERVHRIARELEPDVVIIEIGGTVGDIESLPFLEAIRQLKSDLGRDNVMYIHVTLVPYLRVANELKTKPTQHSVKELRSIGIQPDVVVCRTEKPLSKEMVDKLALFCDTDRDAVVQAVDASHIYEVPLMLEEEGLGDFTVERLGLEVPKADMNEWEALVERVKSPKRAVTIGLVGKYVELQDSYKSILESFIHAGAENEVKVNIRSIHSEFIDIEGKTKITDLDGILVAPGFGERGIEGKIEAVRFARENNLPFFGICLGMQMAVIEYARNVLNLPDANSTEMEAQTKNPVIDIMESQKNITQMGGTMRLGAWECELKEGTKVFEAYGKSTISERHRHRYEYNNKYKEQLEAAGMLSSGTNPQTGLVEVVEIPTHPWFIGVQYHPEYKSTVKTPHPLFIGFIRAAIDHA",
    "response#2": "KYVFVTGGVVSGLGKGITAASLGRLLKARGYKVTMQKFDPYLNVDPGTMNPIQHGEVFVTDDGTETDLDLGHYERFIDESLNHNSNVTSGKVYWSVINKERHGDYGGGTVQVIPHITNEIKSRFYRPENPEEGNTVAIIEVGGTVGDIESQAFFEAIRQFQIEVGPGNSCMIHVTLVPYLYGSRELKTKPTQQSVKELQQMGIRADVIVCRSEFEIPQQLKDKIALFCNVPSSHVLQNLNAESIYEVPLMMEKEHLAQAVCECLNLQDSYKSILESFIHAGAENEVKVNIRSIHSEFIDIEGKTKITDLDGILVAPGFGERGIEGKIEAVRFARENNLPFFGICLGMQMAVIEYARNVLNLPDANSTEMEAQTKNPVIDIMESQKNITQMGGTMRLGAWECELKEGTKVFEAYGKSTISERHRHRYEYNNKYKEQLEAAGMLSSGTNPQTGLVEVVEIPTHPWFIGVQYHPEYKSTVKTPHPLFIGFVKNALDAQW",
    "response#3": "KFIFVTGGVVSSLGKGITAASLGRLLKSRGLKVAIQKLDPYINIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDASLGKSSNVTTGGIYWSVISKERKGDYLGATVQVIPHITNEIKERVHRIARELEPDVVIIEIGGTVGDIESLPFLEAIRQLKSDLGRDNVMYIHVTLVPYLRVANELKTKPTQHSVKELRSIGIQPDVVVCRTEKPLSKEMVDKLALFCDTDRDAVVQAVDASHIYEVPLMLEEEGLGDFTVAPMFNIAPKPDHSEYVEIALVGKYTQLHDAYLSVVEALHHGGIANRRNVRIRWVDSEEVEWQGCDALLEGIDGMIIPGGFGQRGTEGMILAADYARVHKIPYLGICLGMQMAVVAFARNVLAMHDANSSEFAPDTIHPVIDLMPEQKDVENLGGTMRLGAYPCTLEKDSLAFRLYGQETISERHRHRWEFNNDYRDAMQEAGMRLSGMSPDGRIVEMVELRDHPFYIGTQAHPEFKSRPNHAHPLFAGLLSAALTHKK"
  },
  {
    "reference": "MPRATALGALVSLLLLLPLPRGAGGLGERPDATADYSELDGEEGTEQQLEHYHDPCKAAVFWGDIALDEDDLKLFHIDKARDWTKQTVGATGHSTGGLEEQASESSPDTTAMDTGTKEAGKDGRENTTLLHSPGTLHAAAKTFSPRVRRATTSRTERIWPGGVIPYVIGGNFTGSQRAIFKQAMRHWEKHTCVTFIERTDEESFIVFSYRTCGCCSYVGRRGGGPQAISIGKNCDKFGIVAHELGHVVGFWHEHTRPDRDQHVTIIRENIQPGQEYNFLKMEAGEVSSLGETYDFDSIMHYARNTFSRGVFLDTILPRQDDNGVRPTIGQRVRLSQGDIAQARKLYKCPACGETLQDTTGNFSAPGFPNGYPSYSHCVWRISVTPGEKIVLNFTSMDLFKSRLCWYDYVEVRDGYWRKAPLLGRFCGDKIPEPLVSTDSRLWVEFRSSSNILGKGFFAAYEATCGGDMNKDAGQIQSPNYPDDYRPSKECVWRITVSEGFHVGLTFQAFEIERHDSCAYDYLEVRDGPTEESALIGHFCGYEKPEDVKSSSNRLWMKFVSDGSINKAGFAANFFKEVDECSWPDHGGCEHRCVNTLGSYKCACDPGYELAADKKMCEVACGGFITKLNGTITSPGWPKEYPTNKNCVWQVVAPAQYRISLQFEVFELEGNDVCKYDFVEVRSGLSPDAKLHGRFCGSETPEVITSQSNNMRVEFKSDNTVSKRGFRAHFFSDKDECAKDNGGCQHECVNTFGSYLCRCRNGYWLHENG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. A protein that can perform zinc ion binding, serine-type endopeptidase activity, metalloendopeptidase activity, calcium ion binding in extracellular region, extracellular space to support protein processing, cell differentiation, collagen fibril organization, dorsal/ventral pattern formation.2. The protein requires Zn(2+) to activate a critical catalytic site.3. The active site active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.4. The protein must contain a signal peptide for proper functionality.5. The designed protein have EGF-like 2; calcium-binding, EGF-like 1; calcium-binding, Peptidase M12A, CUB domains with specific sequence motifs that are crucial for its activity.6. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects.7. Target a Polar residues compositional bias in the protein's composition for improved properties.8. The protein must exhibit the following characteristics: Protease which specifically processes pro-lysyl oxidase; Required for the embryonic development. The designed protein sequence is ",
    "response#1": "SGGRWEKTDLTYRILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHEGRADIMIDFARYWHGDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWTIGDDQGTDLLQVAAHEFGHVLGLQHTTAAKALMSAFYTFRYPLSLSPDDCRGVQHLYGPRCGVPDQDGISCNVNAFCYEKTCNNHDIQCKEIFGQDARSASQSCYQEINTQGNRFGHCGIVGTTYVKCWTPDIMCGRVQCENVGVIPNLIEHSTVQQFHLNDTTCWGTDYHLGMAIPDIGEVKDGTVCGPEKICIRKKCASGQDGISCNVNAFCYEKTCNNHDIQCKEIFGQDARSASQSCYQEINTQGNRFGHCGIVGTTYVKCWTPDIMCGRVQCENVGVIPNLIEHSTVQQFHLNDTTCWGTDYHLGMAIPDIGEVKDGTVCGPEKICIRKKCASDGISCNVNAFCYEKTCNNHDIQCKEIFGQDARSASQSCYQEINTQGNRFGHCGIVGTTYVKCWTPDIMCGRVQCENVGVIPNLIEHSTVQQFHLNDTTCWGTDYHLGMAIPDCTCPTGLEGTYCECNPILSLDAATTVGNKIIFFKDRYFGWRNPESPKSNISLISSFWPTLPSGIQAAYEIGDRNQVFLFKDDKYWVISNLRPQRLYPKNVRSLGFPNFMKKIDAAVFNPLIYKTYFFVGNQYWRYDERRQLMDPGYPKFITKDFPGIGPKIDAVYYNNRHYYFFQGSHVSEYDVLSHRVTKRLKHNIKLG",
    "response#2": "SGGRWEKTDLTYRILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHEGRADIMIDFARYWHGDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWTIGDDQGTDLLQVAAHEFGHVLGLQHTTAAKALMSAFYTFRYPLSLSPDDCRGVQHLYGCTCPTGLEGTYCTCPTGLEGTYCTCPTGLEGTYCQHSENTAFCSNICTNRINCQHGGYADPNNCGQCTCPTGLEGTYCEIPDICDGHFDTIAVLRGEKFVFKDKWFWRVRNNKVLPNYPMPIGHFWKGLPSNIDAAYERTDGKFVFFKGDKYWVFSESTLEKDYPKNLGDLGTGLPKDRIDAALLYTHTGQTFFFKGSQYFRYNEKTRRVDRDYPKSINTWTGAPDNIEAVIMSEDGSYTYFYKDNKYWKFNNQHMKVEPGYPKSVLREWMGCTCPTGLEGTYCEIPDACEASFDAVSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQGLPSPVDAAFEDAQGHIWFFQGAQYWVYDGEKPVLGPAPLTELGLVRFPVHAALVWGPEKNKIYFFRGRDYWRFHPSTRRVDSPVPRRATDWRGVPSEIDAAFQDADGYAYFLRGRLYWKFDPVKVKALEGFPRLVGPDFFGCSYRFTEMTGEITSPNYPSNYEDNTACVYEIEGPYGSTIELTFLDMEIETETLCRYDAVEVRKDDINSIGEKFCGNTLPPVQISSSNQMMVSFTSDPSITRRGFKATYV",
    "response#3": "RWEKTDLTYRILRFPWQLVQEQVRQTMAEALKVWSDVTPLTFTEVHEGRADIMIDFARYWHGDDLPFDGPGGILAHAFFPKTHREGDVHFDYDETWTIGDDQGTDLLQVAAHEFGHVLGLQHTTAAKALMSAFYTFRYPLSLSPDDCRGVQHLYED"
  },
  {
    "reference": "MTYENNTHQQNGAGTADIESRFANMSVQNGAAGGAPRKAYVPPHLRSQQQPASLNSSAASFSPRAPAAFNGNGNSNGNGNGPAAPAAPAAFQSFNRGAPRGGAGGWDAPSSGGYGGARNGGGYGGGARNDGFGQWKDGKHVPGPHNPRLQKELFGEEGDGLHQSMGINFDKYGDIPVEATGRDVPEPVTTFTSPPIDAHLLENIKLARYTNPTPVQKYSIPIIKLGRDLMGCAQTGSGKTGGFLFPILSALFTHGPPPPSAAEMAQGGYNRRKAYPSTLILAPTRELVSQIHDEARKFTYRSWVKPAVVYGGADIVTQLRQIERGCDLLSATPGRLVDLMERGRISLSNVRFLVLDEADRMLDMGFEPQIRRIVEGEDMPGVMDRQTLMFSATFPRDIQLLAKDFLKEYVFLSVGRVGSTSENITQKIEYVEDDDKRSVLLDVLASMPSGGLTLIFVETKRMADMLSDFLLRSKIGATSIHGDRTQRERERALELFRSGKTPIMVATAVAARGLDIPNVTHVVNYDLPSDVDDYVHRIGRTGRAGNVGHATAFFNRGNKNIVRDLIELLKEANQEVPQWLEAVARESMFGAGGGSRGGRGRGRGRGGASSFGNRDARTMNGPSSGGGRGFGGGYGGGMGGGLGGSYGAGAAAYGGPPPASNGGSYGGNSSWW",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein have Helicase ATP-binding domains with specific sequence motifs that are crucial for its activity.2. A protein with RNA helicase activity, ATP hydrolysis activity, RNA strand annealing activity functions is required to enable positive regulation of formation of translation preinitiation complex, spliceosomal complex disassembly, RNA-mediated gene silencing, cytoplasmic translational initiation.3. For general function, the protein need meet that ATP-binding RNA helicase involved in translation initiation4. The protein design should include a Q motif motif to ensure proper function. The designed protein sequence is ",
    "response#1": "GFEKPSAIQQRAVLPIISGRDVIAQAQSGTGKTSMISLSVCQIVDTAVREVQALILSPTRELAAQTERVMLAIGDYINIQVHACIGGKSIGEDIRKLEHGVHVVSGTPGRVCDMIKRRTLRTRAIKLLILDEADEMLGRGFKDQIYDVYRYLPPELQVCLISATLPHEILEMTSKFMTDPVRILVKRDELTLEGIKQI",
    "response#2": "FDDMNLREELLRGIYGYGFEKPSAIQQRAIIPCVRGRDVIAQAQSGTGKTATFSIAILQQIDTSIRECQALILAPTRELATQIQRVVMALGEYMKVHSHACIGGTNVREDARILESGCHVVVGTPGRVYDMINRKVLRTQYIKLFVLDEADEMLSRGFKDQIQDVFKMLPPDVQVILLSATMPPDVLEVSRCFMRDPVSILHVGQIGEAVDVVGIKQFFVAVEKEEWKFDTLCDLYDTLTITQAVIFCNTRRKVDWLSAKMKENNFQVSSMHGEMQQKERDEVMAEFRQGSSRVLITTDVWARGIDIANISLVINYDLPTNRENYIHRIGRSGRFGRKGVAINFVTVDDVRTLRDIEQFYSTQIDEMPVKLEDMARK",
    "response#3": "GFERPSPIQEESIPIALTGRDILARAKNGTGKTAAFCIPVLEKIDQDNNVIQAVIIVPTRELALQTSQVCKELGKHLKIQVMVTTGGTSLKDDIMRLYQPVHLLVGTPGRILDLTKKGVCVLKDCSVLVMDEADKLLSQEFQPSVEHLISFLPESRQILMFSATFPVTVKDFKDRFLTNPYVINLMDELTLKGITQIGFIKCTPIQAKALPVLLAGQDIAGQAQTGTGKTLAFLTATFNHLLTTPAPEGRKITQPRAIIMAPTRELAIQIFNDAESLIASTGLKAALAYGGERYEKQQQVIEQGVDILIGTTGRIIDFYKQGHIDFKMIQAVVLDEADRMFDLGFIKDIRFIFRRMPAPTERLNMLFSATLSYRVQELAFEHMQEPEHVVVEPEQKTGHRIKEGFERPSPIQEESIPIALTGRDILARAKNGTGKTAAFCIPVLEKIDQDNNVIQAVIIVPTRELALQTSQVCKELGKHLKIQVMVTTGGTSLKDDIMRLYQPVHLLVGTPGRILDLTKKGVCVLKDCSVLVMDEADKLLSQEFQPSVEHLISFLPESRQILMFSATFPVTVKDFKDRFLTNPYVINLMDELTLKGITQIGIKQFYVNVKQENWKLGTLCDLYDTLSITQSVIFCNTRRKVDQLTQEMSIHNFTVSAMHGDMEQRDREVIMKQFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPSNRENYIHRIGRGGRFGRKGVAINFITDDDRRILKDIEQFYHTTIEEMPANIADL"
  },
  {
    "reference": "MQDHNKKAMAGLTLAALGVVYGDIGTSPLYTLRECFVSQNLPTTPDNIFGILSLIFWSLIFVVSVKYVAFVLRADNRGEGGIMALMALARHYTTHAARWKIVLLGLFGAALFYGDAIITPAVSVLSAAEGMEVVSSGMEAYVLPMAVGVLVGLFLLQRHGTARVGLMFGPVMMVWFAILGILGLHQIIQQPAVLQALNPWHAVTFLSQHGFHAFLTLGSVVLALTGAEALYADMGHFGKTPIRRAWFSLVLPGLGLNYFGQGALLMSNPAAIKNPFFLLAPDWALLPMIALATLATVIASQAVISGAYSLTRQAILLGYCPRLEVHHTSDKEIGQIYMPFINWALLVAVLVVVLTFKNSSSLAAAYGIAVTGTMLITTMLFFVVARVNWRWPLPLALGITLLFGVIDTAFFAANVHKVADGGWLPLVMGMAIFTLMSTWKQGRDILFKRLREQALPLDDFIHNLEAYPPARVEGTAVFLTSTLHGVPHALLHNLKHNKVLHERVVLMTVRTEDIPYVPEDERLEIVQMSASFWRVMARYGFKEEPNVVEVLDKCAKEGFEMELMDTSFFLSRETIVSTGHPGMARWRQKIFLWMSKNALRATDFFQVPTNRVVELGAQVEL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Transport of potassium into the cell.2. The protein have enhanced H(+)(in) + K(+)(in) = H(+)(out) + K(+)(out).3. The protein can be expressed in plasma membrane and perform symporter activity without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "IVYLTSDPEMKRVDTDIFFSIFADHPKRARAWWAVSVETSDNPFTREYSVENFGTDFLFRVRIRLGFKVSQSIPAYIHQIMNDLSKSGDLPKQTTRYPKLDADPNIGPIRYVLIHKALMPESKVSQKGAISLQIKYAIRHLAGSPVKWFGLAPYNPLIH",
    "response#2": "IVYLTSDPEMKRVDTDIFFSIFADHPKRARAWWAVSVETSDNPFTREYSVENFGTDFLFRVRIRLGFKVSQSIPAYIHQIMNDLSKSGDLPKQTTRYPKLDADPNIGPIRYVLIHKALMPESKVSQKGAISLQIKYAIRHLAGSPVKWFGLAPYNPLIS",
    "response#3": "IVYLTSDPEMKRVDTDIFFSIFADHPKRARAWWAVSVETSDNPFTREYSVENFGTDFLFRVRIRLGFKVSQSIPAYIHQIMNDLSKSGDLPKQTTRYPKLDADPNIGPIRYVLIHKALMPESKVSQKGAISLQIKYAIRHLAGSPVKWFGLAPYNPLIF"
  },
  {
    "reference": "MTKKYVVALDQGTTSSRAIIFDHDANIVSVSQREFTQIYPQAGWVEHDPMEIWASQSSTLIEVIARSGIHASEIASIGITNQRETTVIWDKQTGKPVYNAIVWQCRRSSEICEELKSQGLEAYIRDTTGLLLDPYFSGTKIKWILDNVSGVRERAERGELLFGTIDTWLVWKLTEGKVHVTDPTNASRTLLFNIHTQSWDKRILEALTIPESLLPQVKPSSAVYGKTRIAGEGGEISIAGIAGDQQSALFGQLCTEPGMAKNTYGTGCFLLMNTGEKAVKSNHGLLTTIAIGAKGEVNYALEGSVFMGGATIQWLRDELGLIRDAQDTEYFASRVESTNGVYLVPAFVGLGAPYWDPSARGALVGLTRGSNRNHIIRAALEAIAYQSRDLLDAMAKDSGVELKKLKVDGGAVANDFLMQFQADITSVEVQRPAVTETTAMGAAFLAGLAVGFWDSTSELKHRADIDKSFMPSISEEQKDELYAGWQKAVSQTING",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate, ATP binding.2. The protein must exhibit the following characteristics: Key enzyme in the regulation of glycerol uptake and metabolism; Catalyzes the phosphorylation of glycerol to yield sn- glycerol 3-phosphate..3. The designed protein should interact with enzymes and substrates within the Polyol metabolism, glycerol degradation via glycerol kinase pathway, sn-glycerol 3-phosphate from glycerol: step 1/1 pathway to enhance or regulate its activity.4. The designed protein should be optimized for ATP binding, glycerol kinase activity activity in order to improve phosphorylation, glycerol catabolic process, glycerol-3-phosphate metabolic process, glycerol metabolic process. The designed protein sequence is ",
    "response#1": "KNTYGTGNFLVLNTGTELVRSQHGLLTTVAYQFGDNPPVYALEGSIAVTGSAVQWLRDQMKIIKTAAESEELARTVEDNGGMYFVPAFSGLFAPYWRSDARGAIVGLARYNDNAHLARATLEAICYQSRDVVVAMEQDSSVHLDVLRVDGGVTANDLCMQIQADILGVPVSRPVVAETTALGAAYAAGLVASGLL",
    "response#2": "KNTYGTGNFMLLNTGTTPVFSKHGLLTTVCYRLDDQPAVYALEGSIAVTGSLVQWLRDNLGLISSADEIEPLARSVDDNGGAYIVPAFSGLFAPRWRPDARGVIAGLTRFVTKAHLARAVLESTAFQTREVVDAMRADAESQQLDLELTTLKVDGGMTANDLLMQFQSDILDVPVVRPVVAETTALGAAYAAGLL",
    "response#3": "KNTYGTGNFMLLNTGTTPVFSKHGLLTTVCYRLDDQPAVYALEGSIAVTGSLVQWLRDNLGLISSADEIEPLARSVDDNGGAYIVPAFSGLFAPRWRPDARGVIAGLTRFVTKAHLARAVLESTAFQTREVVDAMRADAESQQLDLELTTLKVDGGMTANDLLMQFQSDILDVPVVRPVVAETTALGAAYAAGLIGGLY"
  },
  {
    "reference": "MPSPRNALAIVLAAGEGTRMKSDTPKVLHQVAGRSMLAHVLAAVAEAEVGTVAIVMGPGRDDVRVEALRLTPGARTFVQSERLGTAHAVLAAREAIAAGYDDLLVLFADTPLVSGGTIRALREALAQGAGVAALGFIAADPFGYGRLLQDASGRLVAIREEKDASETERAVKLCNAGLMALDGKSALDWLGRIGNANAKGEYYLTDVVEMARAAGREARVVLADESEVLGVNDRIQLAQAEAVAQSRLRRAAMAAGVTMIAPETVFLCADTVFGRDVLLEPNVVIGPGVTIGDKTVIHAFSHLEGAKVGADAAIGPFARLRPGAALAEAAKVGNFVEIKNANVAKGAKVNHLTYIGDADIGANANIGAGTITCNYDGFFKYRTVIGENAFIGSNSALVAPVTVGAGAYVGSGSVVTKDVTADALAVARGRQMEKAGWAANFRSVQTDKKAKK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for Mg(2+) binding.2. A protein that can perform magnesium ion binding, glucosamine-1-phosphate N-acetyltransferase activity in cytoplasm to support peptidoglycan biosynthetic process.3. For general function, the protein need meet that The C- terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N- acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5- triphosphate), a reaction catalyzed by the N-terminal domain.; Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc)4. The designed protein should interact with enzymes and substrates within the UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1, Nucleotide-sugar biosynthesis pathway to enhance or regulate its activity. The designed protein sequence is ",
    "response#1": "MAAGKGTRMKSKTPKVLQKLAGRALLQHVLDTAAQLQARSAVVITGHGAQEVEAAVAQGRSADVLDLKFVRQEPQLGTGHAVQQAVPVLADDGMVVVLSGDVPLTQAAILQDLVTAAAGTRMALLTVTLDDPTGYGRIVRENGQVTGIVEHKDASPEQLAIREINTGILAARTEHLKRWVAALETDNAQGELYLTDIVGLAAAEGVPIATAQPEAPIEVEGVNDRVQLARLERAHQARVVERLMLEGVRLADPARFDLRDDVRGNPAELVCAQDVEIDVGCIFTGRVEIGEGARIGAYCHISNATIAAGAVIHPFTHIDGEKLGASVGEGALIGPFARLRPGAQLGKEVHIGNFVEVKNSTLADGAKANHLAYLGDATVGERVNYGAGSITANYDGANKHRTVIEADVHVGSNCVLVAPVTLGAGGTIGGGSTITKNTEAGALTVARGKQRNISGWQRPVKK",
    "response#2": "MAAGKGTRMKSKTPKVLQKLAGRALLQHVLDTAAQLQARSAVVITGHGAQEVEAAVAQGRSADVLDLKFVRQEPQLGTGHAVQQAVPVLADDGMVVVLSGDVPLTQAAILQDLVTAAAGTRMALLTVTLDDPTGYGRIVREQGRVVAIVEERDATAEQRAIREVYTGMMAAPTARLKAWVAGLDNNNAQQEYYLTDIVAMAVADGVPVVGAQAEDAWETLGVNSKAQLADLERVHQRGVADALLTEGVHLIDPARFDLRGHLTIGKDVRIDINVIIEGECELGDNVEIGAGCIIKNTKISANTKVQPYSVFDNAFVGESVQIGPFARLRPGAKLANEVHIGNFVEVKNSSIGFASKANHFAYLGDAQIGSGSNIGAGTITCNYDGANKHKTVIGDDVFIGTNNSLVAPVKIGNGATTGAGSTITRDVADNALAVERSQQVTLEGWQRPVKTAK",
    "response#3": "MAAGKGTRMKSKTPKVLQKLAGRALLQHVLDTAAQLQARSAVVITGHGAQEVEAAVAQGRSADVLDLKFVRQEPQLGTGHAVQQAVPVLADDGMVVVLSGDVPLTQAAILQDLVTAAAGTRMALLTVTLDDPHGYGRVVRQADGTVARIVEQKDASADELAVREINSGIYAFDAAALREGLARLSSDNAQGEKYLTDVLALVRDAGGAVVAEHVTDVWQTEGVNDRVQQATLGAELNRRTLDAWMRAGVTFIDPGSVFVHAGVTIGRDSVIYPNVYLEGNTKIGRKATIFPNVRIVNADIGAGVVIKDSTVIEESQVRKGASVGPFAHLRPGSQVGDRAKVGNFVELKKAVIGKDSKASHLSYLGDTRIGRGVNIGAGTITCNYDGTHKHTTVLEDGVFVGSDTQLVAPVKVGRGSYIGAGSTITKDVPPGALALSRTVQRNIDGWVERRRPGTPAADAAQRALSGPRDGSGTDTGTDTATDTAADRTR"
  },
  {
    "reference": "MPDRKRILRSPWFWIPVAIVVLLVLNPLLRGGASYTKVDTSVALQAIAKNQVKEATQNDKSQSLDLILKAPDSAGHTRITTSYPAGAANQVFSALRVQATASGMKFNTHIGGQSVVLQILFAFAPFLLVGILIFFIISQMQGGGSRVMNFGKSKAKLVSKDTPKTTFADVAGADEAIEELQEIKEFLENPAKFQAIGAKIPKGVLLYGPPGTGKTLLARAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFEQAKANAPAIVFVDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFDTKGGVILIAATNRPDILDPALLRPGRFDRQIAVDRPDLAGRKAILRVHAKGKPLAADVVLDDVARRTPGFTGADLANVINEGALLTARSNAQRVTGAMLEEAIDRVVLGPQRKTRVISEKERKLTAYHEGGHALVGWALPNADPVYKVTILARGRTGGHTLPLPTEDKYTMARSEMIDQLAFALGGRAAEELVFHEPTTGASNDIEKASAIARAMVTEYGMSAKLGAVKYGTGDAEPFMGRDYGHTRDYSDEIAADIDEEVRGLIEGAHDEAWEILVQYRDILDHLVVELMEKETLVKADIERIFAPVKKRQPHNSYTGFGKRTPSDKPPIDIPDELTGASVNGSRPQPTAPAPSPYPREPYVHPDPGADA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.2. The protein should contain a Helical membrane-spanning region.3. The designed protein should have ATP-dependent peptidase activity within plasma membrane to regulate cell division, protein catabolic process, proteolysis.4. The presence of AAA+ ATPase domains is critical for the interaction of the designed protein with its intended targets.5. Target a Pro residues compositional bias in the protein's composition for improved properties.6. The ATP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "VLLVGPPGTGKTLLAKATAGEAGVSFFETSGSEFVEMFVGVGAARVRDLFEQARKAAPAIVFIDEIDAIGQSRAGTVRIGGNDEREQTLNQLLGEIDGFKTNRERPVIIMAATNRPEILDPALLRAGRFDRQIQVHIGQGIIK",
    "response#2": "LLVGPPGTGKTLLAKAVAGEANVPFFSMSGSDFVEMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAIGRARGRNPNMGSNDERENTLNQLLTEMDGFDTNSGVIILAATNRADMLDRALMRAGRFDRQIHVEFASDVDPMLLAKGTPGFSGADLENLVNEAALLAAKRGQEKLSMKDFESDVDLEIIARGTPGMSGADIANMVNEAALLAARRNKDKVSMTEFDQ",
    "response#3": "VILYGPPGTGKTLLAKAVSGEADVPFIEVSGSSFDDMFVGVGAKRVRELFAKAKKVSPCIIFIDEIDAVAAKRGNKYMGGGVNDQTINQLLSEMDGFNTMSGIVIMAATNKLDSIDEAILRPGRFDRHIQVL"
  },
  {
    "reference": "MSAMTPREIVHELDQHIIGQAKAKKAVAIALRNRWRRMQLSDDLRNEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEIGYVGKEVETIIRDLVDVAIKMTREQQTKKFKHRAEEAAEERILDALLPPAKDQYGESQRDDSSSTRQTFRKKLREGQLDDKEIEIDLAQAQPNVEIMAPPGMEDMTNQLQSMFQNMGGDKRTKRKLKIKEAFKLLSEEEAAKLVNPEELKEQAIFAVEQNGIVFIDEIDKICKRGDSSGPDVSREGVQRDLLPLIEGCTVSTKHGMVKTDHMLFIASGAFQMAKPSDMIPELQGRLPIRVELEALTADDFKRILTEPHASLTEQQRELLKTEQVTIEFSDDAIERIAKAAWQVNEKTENIGARRLHTVMEKLMEEISYDASEQAGTSLVVDAAYVEKHLGALVEDEDLSRYIL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that contributes to protein unfolding by functioning in HslUV protease complex with ATP binding, ATP hydrolysis activity, proteasome-activating activity, peptidase activity.2. The ATP binding site should be located in a region of the protein that is accessible to the ligand.3. The protein must exhibit the following characteristics: HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis.; ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity; The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV.4. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "MAPPGMEEMTNQLQSMFQNLAGQKQKARKIKIKDAFKLLIEEEAAKLVNPEELKQQAIEAVEQHGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQVASPSDLIPELQGRLPIVQVATPPGMEEMTSQLQNLFAGLGKGKKKSRKLKVADALKLVRDEEAARLVNEEELKARALEAVEQHGIVFIDEIDKVAKRGNTGGADVSREGVQRDLLPLIEGCTVNTKLGMVKTDHILFIASGAFHLAKPSDLVPELQGRLPIDITYAHGLTKEDFVRILKEPKNALTKQYQELLRTEGVEIKYTEEAIETIAEVAYLINQQSEDIGARRLHTVMEKLFEDLSFHAPELSGQEIVITADYVKEQLGRGGILKEVL",
    "response#2": "MPKNILMIGPTGVGKTEIARRLAKLAGAPFVKVEATKFTEVGYVGRDVEQIIRDLVEIAISLVREKKRDEVQERAHINAEERVLEALVGKTASPATRDNFRQKLRAGELDDKEIEIEVANNSNNSAPTFDIPGMPGAQMGIMNLSDIFGKIGGRTKIRKTTVKDAFKPLIDDESEKLLDQDQIIQEALCITENDGIVFIDEIDKIATQDGGAGAAISREGVQRDLLPLVEGTIVATKYGQIKTDHILFIASGAFHVSKPDFLYIHPLGEEEFFRILTEPKNALIRQYEALMETEGIRLHFTEEAIREIARIATQVNSQTENIGARRLHTIMEKLLEELSFEAPEHREQSVEIDVDYVRKQILMIGPTGVGKTEIARRLAKLIKAPFIKIEVTKFTEVGYVGRNVESMVKDLVAESIQLVEEEKHEEVSKQARKQAENRIIDLLLSEKGV",
    "response#3": "MAPPGMEEMTNQLQSMFQNLAGQKQKARKIKIKDAFKLLIEEEAAKLVNPEELKQQAIEAVEQHGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQVASPSDLIPELQGRLPIRHIMPTLGEEEFFRILTEPKNALIRQYEALMETEGIRLHFTEEAIREIARIATQVNSQTENIGARRLHTIMEKLLEELSFEAPEHREQSVEIDVDYVRKQINCYRGLRHPLGLPVMQFTNCK"
  },
  {
    "reference": "MGCARDAILEALENLTANELKKFKLKLLSVPLRDGYGRIPRGSLLPMDAVDLTDKLVSFYLEGYGAELTAAVLRDMGMQEMAKQLQEVMLKGPGAAPAGIKAPAQIAAKPALHFLDQHRAALITRVTDVDGVLDALYGKVLSEEQYQAVRAETTNPMKMRKLFSFAPAWNQTCKDLLLQALRETQPYLVADLESR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have tropomyosin binding, myosin I binding, interleukin-6 receptor binding, protein homodimerization activity within nucleoplasm, mitochondrion, NLRP3 inflammasome complex, extracellular region to regulate positive regulation of antigen processing and presentation of peptide antigen via MHC class II, cellular response to interleukin-1, regulation of tumor necrosis factor-mediated signaling pathway, negative regulation of protein serine/threonine kinase activity.2. The designed protein should contain one or more CARD, Pyrin domains that are essential for its function. The designed protein sequence is ",
    "response#1": "GGIMVNGSRLKNLVLTYVNAISSGDLPCIENAVLALAQRENSAAVQKAIAHYDQQMGQKVQLPMETLQELLDLHRTSEREAIEVFMKNSFKDVDQSFQKELETLLDAKQNDICKRNLEASSDYCSALLKDIFGPLEEAVKQGIYSKPGGHNLFIQKTEELKAKYYREPRKGIQAEEVLQKYLKSKESVSHAILQTDQALTETEKKKKEAQVKAEAEKAEAQRLAAIQRQNEQMMQERERLHQEQVRQMEIAKQNWLAEQQKMQEQQMQEQAAQLSTTFQAQNRSLLSELQHS",
    "response#2": "GGIMVNGSRLKNLVLTYVNAISSGDLPCIENAVLALAQRENSAAVQKAIAHYDQQMGQKVQLPMETLQELLDLHRTSEREAIEVFMKNSFKDVDQSFQKELETLLDAKQNDICKRNLEASSDYCSALLKDIFGPLEEAVKQGIYSKPGGHNLFIQKTEELKAKYYREPRKGIQAEEVLQKYLKSKESVSHAILQTDQALTETEKKKKEAQVKAEAEKAEAQRLAAIQRQNEQMMQERERLHQEQVRQMEIAKQNWLAEQQKMQEQQMQEQAAQLSTTFQAQNRSLLSELQHR",
    "response#3": "RTRLALIICNSEFDYLSKRQGSEIDILGMQDLLENLGYSVVVKENLSALEMKTELKNFAARQEHKFSDSTFLVFMSHGTLDGICGTKHRNEEPDILHDDTIFQIFNNRNCRSLKDKPKVIIMQACRGRR"
  },
  {
    "reference": "MPPRRAGQPFRRVGKLGNTSYGPRKPKTVDASSLRSGEGTSQNEKLEATRLANRIDESMGFARFESGKKKVGWLVNMHSTALEDENVPGGRAGVDFYFIGEEGDTFKATLEYDPYFLLAVKKGREQEVEEFCRRSYEGLIKNIKRVEKEDLSMPNHLTGYRRTFLQLSFANVNDLLAVRKVVTPVIEKNKKNINAMDTYAEVASGNVGMDFFDNDDYEHEKRFTTTVDASDFIVDIREYDVPYHVRVAIDKDVRIGNWYTVEAKHGIITITCIEDRLTPADPVVMAYDIETTKLPLKFPDAVIDQIMMISYMIDGQGFLITNREIVSEDIADFEYTPKPEYQGPFMIFNEPDEKAVIERFFEHIKEAQPTVMVTYNGDFFDWPFVEARASVLGIDMYREIGFKKNSEDIYQSNYCAHMDAFSWVSRDSYLPQGSRGLKAVTMAKLGYDPDELDPELMTPYAAERPQTLAEYSVSDAVATYYLYMKYVHPFIFALCKILPLGPDDALRKGTGTLCEMLLMVQAYQKGIVLPNKHQAPKEAFWEGHLLESETYVGGHVEALEAGVFRSDIPYNFAVDPTAIDELLRDLDAALKFSIEVEEKKKLEDVTNYDEIKAEIVEKLLQLKATPNRSERPLIYHLDVASMYPNIMTTNRLQPDSMIKEANCAACDFNRPGKTCDRRMPWAWRGEYLPAKRDEYNMVRNALENEKFPGKYPNSPMRTFQQLSEDEQAEKVRKRLTDYSKKIYHKVHDATTIEREAIICQRENPFYVD",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should contain one or more DNA polymerase epsilon catalytic subunit A C-terminal domains that are essential for its function.2. The designed protein should have nucleotide binding, zinc ion binding within epsilon DNA polymerase complex, chromosome, telomeric repeat region to regulate double-strand break repair via nonhomologous end joining, CMG complex assembly. The designed protein sequence is ",
    "response#1": "MYNPLKYPNPSLQWHYKVLQAMALDEDVPEALDDATIPKYRQIDKRVGGYLAEWKEALTEKAGGLMKSRAMKREAEDEDVSRPLAKRTL",
    "response#2": "KDLSFSVGVYNLIQKAYKPYPVKLYRETNEPVKTKTRVFNGKTGSLLLPSDTKRAQTYGNRQIAMEKEETEEVKRFDSPGLFLIGFKPLSMLKQHHHIRPSQFMYPEESLVTGSTTLFNALLMKCLEKEVMALCRYIARRNTPPRIVALIPQEEEVDEQKVQIAPPGFHIIFLPYADDKRNVDFTEKVPANREQVL",
    "response#3": "YFSNLHFELAPNVTISVKGYMPLHRQQPARTCYVWLGGEQAQLAQSETIKVDSATRTVDKSEVKKAYKFGGEYIHFKPEEAASLKNLGGKVLRVIGFKPRSLLPTWASVKKSIFIFPSEEHFVGSTPRENANPIMVAIIPSRAMDDETSETPYLPAGLWLYPLPFADDVRNVDLAAPPRPADELTDKMREIVQNLQLPKAMYNPLKYPNPSLQWHYKVLQAMALDEDVPEALDDATIPKYRQIDKRVGGYLAEWKEAC"
  },
  {
    "reference": "MSKQFILAIDQGTTSSRAIIFDKKGNIKKIAQKEFTQIYPKSGWVEHDPMEIWASQSSIVREALEYARVSPRDIAAIGITNQRETTVVWDKNTGQPVYNAIVWQCRRTSHICDEINKNEDLKKYIRKNTGLIVDAYFSATKIKWILDNVDGAREKAEKGDLLFGTIDTWLIWNLTKGEVHATDFSNASRTMLFNINTLEWDKKILEYLDIPVSMLPEVRSSSGDFGHTHPSTLGGARIPIAGVAGDQQSALFGHCCFEEGMAKNTYGTGCFALMNVGDKPVFSDAGLLTTIAWAEDGKPTYALEGSVFIAGAVIQWIRDGLGLVRSAEDSEYYATKIDSTDGVYLVPAFVGLGTPYWDMYARGTIVGITRDTRREHIIRAALEAIAYQAKDVLDCMKEDTGLDWAGLRVDGGAVQNNFLMQFQSDILQSEISKPKINEITGLGAVFLAGLAVGFWKDKEELKTILTTEKTFEPKKDPETVAHDYKGWKKAVQRSMAWAE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for substrate binding.2. Ideal protein should have a binding affinity for key enzymes in the sn-glycerol 3-phosphate from glycerol: step 1/1 pathway to enhance the overall efficiency of the pathway.3. The protein must exhibit the following characteristics: Key enzyme in the regulation of glycerol uptake and metabolism. The designed protein sequence is ",
    "response#1": "KNTYGTGNFLVLNTGTELVRSQHGLLTTVAYQFGDNPPVYALEGSIAVTGSAVQWLRDQMKIIKTAAESEELARTVEDNGGMYFVPAFSGLFAPYWRSDARGAIVGLARYNDNAHLARATLEAICYQSRDVVVAMEQDSSVHLDVLRVDGGVTANDLCMQIQADILGVPVSRPVVAETTALGAAYAAGLY",
    "response#2": "KCTYGTGSFLVAHTGDRPIRSRHQLLTTVAWSDRTSTGYALEGALFTTGASVQWLRDGLGIIETADESEALAASVPDSGGVYFVPALSGLGAPHWDMGARGLLIGLTRGSGRGQIARAVLEAIAFQTREVTDALAADMGTPLTRLKVDGGAVRNNLLMQLQADVLGVPVERPQLIDTTAQGAAFAATR",
    "response#3": "KCTYGTGSFLVAHTGDRPIRSRHQLLTTVAWSDRTSTGYALEGALFTTGASVQWLRDGLGIIETADESEALAASVPDSGGVYFVPALSGLGAPHWDMGARGLLIGLTRGSGRGQIARAVLEAIAFQTREVTDALAADMGTPLTRLKVDGGAVRNNLLMQLQADVLGVPVERPQLIDTTAQGAAFAACG"
  },
  {
    "reference": "MPLKKSRKTKFIFVTGGVVSSLGKGLAAASLGTLLEKRGHKVLLQKFDPYLNVDPGTMSPFQHGEVYVLDDGAETDLDLGHYERFTSGVLSKLNNLTSGQVYETVIKKERKGDYLGKTVQVIPHVTNEIKGRIYEVEKRMDADIIITEIGGTVGDIEGLPFLEALRQFVQEVGPDNALFIHVTLVPYIGAAGELKTKPTQQSIAKLREIGIQPQVVLLRSELPVEEDARDKISMFCNVPSEAVIECRDVPHSIYELPLYLHEEKLDDIVCDRLGLPKKKPDLKAWQEFVWHVIDPSHETKIAVVGKYIELQDAYKSIYEALTHAGAANDCRVLVERIDAEDLENVEDIEEKLDGIDGLLIPGGFGDRGTEGKINAANFARTRGIPYFGICLGMQIATIEFARNVAKLEEANSTEFDQGSPHPVICLLEEQKSVTHKGASMRLGTWVCDLKPKTRSFDLYNSSTINERHRHRFEFNSDYRERLEKAGLIIAGTSPDGSLVEIIELENHPFYVGAQFHPEFLSKPNYSHPLFRGFVAAALSRRXGIAKHLEQKASSK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen2. The Mg(2+), UTP, ATP, CTP, L-glutamine binding site should be stable and able to withstand changes in environmental conditions.3. The designed protein must have at least one CTP synthase N-terminal, Glutamine amidotransferase domain.4. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile; for glutamine hydrolysis, Nucleophile, active site to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).5. The designed protein should interact with enzymes and substrates within the Pyrimidine metabolism, CTP from UDP: step 2/2, CTP biosynthesis via de novo pathway pathway to enhance or regulate its activity.6. A protein with CTP synthase activity, glutaminase activity, metal ion binding, ATP binding is needed to enhance glutamine metabolic process, 'de novo' CTP biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "KFIFVTGGVLSSLGKGITSASIATILKQSGQKVSMLKIDPYLNVDPGTMSPLEHGEVFVTADGAETDLDLGNYERFIDTTLSAQNNFTTGQVYQSVIKREREGGYLGKTIQVIPHVVDEIKDRIFAAAKGYEFLIVELGGTVGDIEGLPFMEAIREIRHELPKTNTMNIHVTLVPYISAAGELKTKPTQHSVQELRRIGITPHMIVCRTEKPLPKELKKKLAISCDIDNNAVIEAGDAQSIYQVPMNFLKEGILTPICEHFNLEPRAPDLTEWRKLVERIRSPKRSVRISLVGKYTQLHDAYLSVMESLKHAGWENQSRVSISWIDSECVTPDSVDDLLAGSDGILIPGGFGDRGIEGMIVAARYARENKIPYFGICLGMQIAVIEYARDVMGLVDANSSEFAPNGLHSVIDLMPDQMGNIPKGGTMRLGEYPCAIVPETIMNRAYRGEAEIKERHRHRYEFNNVFREQLVKAGLTISGHSPDGHLVEAVELPEHVHPFYLGVQFHPEFKSRPNRAHPVFREFVAAALAARS",
    "response#2": "KYVFVTGGVISGLGKGLTAAAVGALMENRGLKCSFIKLDPYINVDPGTMSPFQHGEVFVTDDGAETDMDLGHYERFASTRMTRDNNFTAGQVYDTVITRERQGGYLGKTVQVIPHITDEIKARVLNAAKGADLLFVETGGTVGDIESLPFLEAVRQLRHELGYQNSVCLHVTLVPFIKAAGELKTKPTQHSVKALREIGIQPEFLVCRTEHPLDEDIKGKLALFCNVEKGHVFEAIDAETIYEVPLLLAEQGIDQRLSEALNVRIGLVGKYTQLEDAYLSVIEALNHAGAHHRAKVEVSWVDSERLGDEEARRELEACDGILIPGGFGIRGIEGKIRASRFAREEEVPYLGICLGMQIAVADFARHVVGLEGANSTEFDPETPHPVIDLLPEQKEVRDMGGTMRLGADPVKLHDGTRAREIYGEAVIYERHRHRYEINNHLRKRLEHAGLVCSGTSPDERLVELVELPEHPFFVASQFHPEFKSRPLRPQPLFREFLATLTEYQGNQDADSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASFVLQDAYKSLNEALVHGGIANRVKVNIRWIDAEIFEHEDSDVAASLEPLHAILVPGAFGERGAEGKIASVKFARERQVPYFGICFGMQMACIEGARNLAGIEKASSTEFGPTDEPVVGIITEWMTAEGVEKREAGGDLGGTMRLGAYDAKLAGNSVVSSIYGATDISERHRHRYEVNTGYRTALEKGGLVFSGMSPDGMLPEIVERPDHPWFVGVQFHPELKSKPFDPHPLFASFI",
    "response#3": "KYVFVTGGVVSGLGKGITAASLGRLLKNRGLKIAAQKLDPYMNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDEDLNKFSNMTSGKVYWNVLTKERNGEYLGSTVQIIPHVTNEIKSFIYAVGRKTNADVVITEIGGTIGDIESQPFLEAIRQVSREVGESNCLFIHVTLVPYLRSSGEHKSKPTQHSVKILQGSGITPHIIVTRSDEPLDQSTKDKIALFCNVKRDCVIENRTVPVLYQAPVMLASEGLDEIVCREMNLQDAYKSILEALIHAGAMNECKVEVRNIHSEYLTKENAKEKLQNLDAVLVAPGFGSRGIEGKIEAIRYARVNKVPFFGICLGMQMACVEFARNVLGMPKAHSTEMDPDTKDGVISMMESQKTITMMGGTMRLGSYACELRPDSTTAEIYGTANITERHRHRYEFNNEYLAAFEEAGFIPVGKNPLTDLVEVVEMKDHPFYIGVQFHPEYKSTVENPHPLFVGFVKESYKSLTEALIHAGAHINAKINIHWCDSERIEDAGAYEVIQTCDAVLVAGGFGHRGVEGKLKAIEYARTNNIPYLGICLGMQLAVIEYARNVLGIEDANSVEFDPEAKNPLIYLIDEFLDQSGNKQVRTHESPMGGTMRLGEYPFTPKKGSKLQKAYGDKETYYERHRHRYEANPKYKEALEKAGMIISGESHGLIEAVELKDHKWFVGVQFHPEFTSHLETPNPIILEFVKESYKSLNEALLHGGIANDARVNLTFIDSEKLSKDNVADTLHPCDALLVPGGFGERGVEGKILAIEYARTHDVPFFGICLGMQLMAIEFARNVCGLKDANSTEFKEAPEHPVITLMADQKNIEKKGATMRLGAYPCKLSAGSKARAIYQSENISERHRHRLEFNNAYREKLSENGMSFCGQSPDGHLVEMMELPEHSWFIGCQFHPEFKSRPTAAHPLFESFV"
  },
  {
    "reference": "MKYNIVLKFEVEGIVDKPDVIGAIFGQTENLFGEEFDLRELQDKGRLGRIYVNINTSKNKTEGEIIIPSNLDRIETALIAAMVENVDKVGPYNARFKLVEIQDIREEKIKKIIDRAKEILGTWTKEKTLDIKEVIYEISSAVKTGEIIEYGPDRLPAGPDVDKDPELIIVEGRADVINLLRYGYKNVIAIEGATGKIPQTIIDLAKQKKTVIAFLDGDHGGDLILKELLANNVKIDYVARAPTGKEVEELTGKEINKSLANMMTVAQYMKKQQEAQQALKEALAPESQPPIVVSKPTTPEQIKEITIKIPQHIIEEIKKLPGTLEGIMFDENWNPIERIQVRDIVQRLENLTDNQNISYIIFDGVITQRLLDLASAKNIKLLVGARIGGISKRPKNVEILTMTDLFTS",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be optimized for DNA-directed RNA polymerase complex, primosome complex-specific folding and stability, while maintaining poly(A) binding.2. The presence of Toprim domains is critical for the interaction of the designed protein with its intended targets.3. For general function, the protein need meet that RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication; Also part of the exosome, which is a complex involved in RNA degradation; Acts as a poly(A)-binding protein that enhances the interaction between heteromeric, adenine-rich transcripts and the exosome.4. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.5. The desired protein should be able to perform ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.6. The protein should be able to bind Mg(2+) ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "INDEDVKAVRDAVPIDAVVSEYLQLRNAGGGNLKGLCPFHDEKSPSFQVSPSKGFFHCFGCQEGGDTITFVMKIDHLTFSEAVERLAGQAGITLRYEEGGYNPSHQRGERIRLVEAHKIAAQWYAEQLATGPEADTGRAFLADRGFDQAAAEHFGVGYSPQGWDHLTRFLRGKGFSDKELLLSGLSQEGRRGPIDRFRGRLMWPIRDIGGDVVGFGARKLYEADNGPKYLNTPDTAIYKKSQVLYGIDLAKKDIAKASRAVVVEGYTDVMACHLAGVTTAIATCGTAFGGDHIKILRRLLMDNGSARVIFTFDGDAAGQKAALRAFEDDQKFAAETYIAIAPDGMDPCDLRLAKGDDAVADLVEPRTPLFEFALRQIVARYDLDTPAGRAAALDEAAPVVARIKNSGAQHEVAVQLERELLKLALQRPELVSPAFDAYGVDEFTAPPYAAVREAIMEAGGAEFGVQDPQDYLVRVREAAPDDTVRAMVTELAVEAIMLHRGVKGVDEVYAGAQLVTVRRRAVERRIRDITGRLTRLDQAVVVEGYFDVIALHAAGIDHAVASLGTALSRQQVKLLSRYSESNQIVLNFDADRAGAKAAERAIGEVEDLAYQGQVQLRVLETEGPVRRSWYAEQLATGPEADTGRAFLADRGFDQAAAEHFGVGYSPQGWDHLTRFLRGKGFSDKELLLSGLSQEGRRGPIDRFRGRLMWPIRDIGGDVVGFGARKLYEADNGPKYLNTPDTAIYKKSQVLYGIDLAYYQKKLKYNGQVAIDYLRNRGLSGEIAKRYHLGYAPEGWHNLEKAFPNNQRELLSTGMLIKSDEGKIYDRYRNRIMFPIHDRNGRVIGFGGRVLDKDQKPKYLNSPETVLFQKSRELYGLHQ",
    "response#2": "IPDRDIAAIRERVCIEEVVGDYVQLRRAGADSLKGLCPFHNEKSPSFHVRPNHGHFHCFGCSEGGDVYAFIQKIEHVSFVEAVEMLADRIGHTINYFGTATSVQRDRGSRSRLIAANAAAAAFYAAALESDEAAPARQYLMERNFDANAARHFGCGFAPSGWDSLTKYLISKGFEFTELEAAGLSRQGRRGPMDRFHRRLLWPILTLAGEVIGFGARRLFDDDPMEAKYVNTPETLLYKKSSVMFGIDLAKRDIVKGHQAVVVEGYTDVMAMHLAGVTTAVASCGTAFGDEHLAMLRRLIMDDNWYRGELIFVFDGDAAGQAAALKAFDGDQQVAGKSFVAVAADGMDPCDLRLSSGDQALRDLVACRKPMFEFAIRSLIPDGMVLDDDPQAKVDALRRSVPLVARIRDYALRDEYARRLWYAEQLATGPEADTGRAFLADRGFDQAAAEHFGVGYSPQGWDHLTRFLRGKGFSDKELLLSGLSQEGRRGPIDRFRGRLMWPIRDIGGDVVGFGARKLYEADNGPKYLNTPDTAIYKKSQVLYGIDLALCDRLGAFYRESLLKNREASEYVKSRGIDPKVARKFDLGYAPSSEALVKVLKENDLLEAYLETKNLLSPTKGVYRDLFLRRVVIPIKDPRGRVIGFGGRRIVEDKSPKYINSPDSRVFKKGENLFGLYEAL",
    "response#3": "IPRTFIDDLLAKTDIVELVNSRVKLKKAGRDYQACCPFHHEKTPSFTVSQKKQFYHCFGCGAHGNAISFLMEYDKLEFVEAIEELAGMLGLEIPRENKPHFHGKQINLQTKRNLYELMQEIAQFYQQQLAQHIPAQSYLQQRGLSPEVISRFQIGFVPNSFDAVLQRFGQQKEDQQKLFDLGMLSRSEQGKIYDRFRHRIMFPIRDRRGKTIAFGGRVLGDEKPKYLNSPESATYHKGNELYGLYEALQANESPEMLLVVEGYMDVVALAQFGVDYAVASLGTATTAEQIQLLFRSTEQAICCYDGDRAGREAAWRAFENALPYLEDGRQLKFVFLPDGEDPDSFIRQHGKASFEQYMQKALSLSEFLFTSLAPQVDFSSKEGKTKLAALAVPLIKKIPGDMLRLSLRNTLAQKGFAILVEGYFDLLRLFSEGIRNVVAPLGTALTQNQANLLSKFTKKVYILYDGDDAGRKAMKSAIPLLLSAGVEVYPVYLPEEPFYEQSLRRPEGAAALDYLRRSRQLQESTIQKFQLGYAPAQWASLATHLIEQKRFPADLVEEAGLVVARRNGQGYYDRFRDRLMIPIHDLQGRVVGFGGRTLTGEEPKYLNSPETTLFEKGKLLFGLDKASRAVVVEGYTDVMACHLAGVTTAIATCGTAFGGDHIKILRRLLMDNGSARVIFTFDGDAAGQKAALRAFEDDQKFAAETYIAIAL"
  },
  {
    "reference": "MSDMVKNLILWLVIAIVLMSVFQSFTPSNGTDSKLDYTTFINEVTQGQVRDVKIESNGVITGTKRSGDTFTTLIPTQYDDGLLDDLISHNVKVEGVPPEEPSLLASIFINWFPMLLLVGIWIFFMRQMQGGGGKGAMSFGKSKARLLSDDQIKTTFADVAGCDEAKEEVAELVDYLRDPSKFQKLGGRIPSGVLMVGQPGTGKTLLAKAIAGEAKVPFFSISGSDFVEMFVGVGASRVRDMFDQAKKSAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQMLVEMDGFEGNEGVIVIAATNRPDVLDPALLRPGRFDRQVTVGLPDIRGREQILKVHMRKVPLGDDVDAAVIARGTPGFSGADLANLVNEAALFAARTSRRLVSMAEFDKAKDKIMMGAERRSMVMSEAEKEMTAYHEAGHAIVGRMVPEHDPVYKVSIIPRGRALGVTMYLPEQDRVSHSKMHLESMISSLYGGRIAEEIIYGAEKVSTGASNDIERATEIARKMVTQWGLSEKMGPMLYADEEGEVFLGRTAAKSKHMSDDTAKDIDEEVRAFIERNYKRAEDILKNNMDILHSMKDALMKYETIDAGQIDDLMARVPVREPKGYGDESTSGKPPSGSANATVDVNKPEENPAN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein can be targeted to plasma membrane and perform ATP hydrolysis activity, ATP binding. The designed protein sequence is ",
    "response#1": "GLKAIDAAIPVGLGQRELIIGDRQTGKTSIAIDTMLNQARSDVLCIYCAVGQRGDAVARVIRALKDGEMLQRCIVMSAGDEEAPGLSYIAPYAAMTMAEYFSAQGQDVLVIFDDMTHHARSYRELSLLLRRPPGREAFPGDIFYVHARLLERAGQFGADIGGGSITTLPVVETQAGNLSAYIPTNLISITDGQIYLSPQLVRKNQFPAVDLGLSVT",
    "response#2": "GLKAIDAAIPVGLGQRELIIGDRQTGKTSIAIDTMLNQARSDVLCIYCAVGQRGDAVARVIRALKDGEMLQRCIVMSAGDEEAPGLSYIAPYAAMTMAEYFSAQGQDVLVIFDDMTHHARSYRELSLLLRRPPGREAFPGDIFYVHARLLERAGQFGADIGGGSITTLPVVETQAGNLSAYIPTNLISITDGQIYLSPQLVRKNQFPAVDLGLSVTQNRTLRGVSGNLRVTLSQFEELEDFARFGTRLDDVTRARLARGSAVRTALRQAERDPMSALEQLAVLIAAMEGLFDQFNEQKINDAMRLVRDAAQQQLAVIEQRIIDNQTLQDDDHTLIVNCAQRGVSGNLRVTLSQFEELEDFARFGTRLDDVTRARLARGSAVRTALRQAERDPMSALEQLAVLIAAMEGLFDQFNEQKINDAMRLVRDAAQQQLAVIEQRIIDNQTLQDDDHTLIVNCAQKALSIL",
    "response#3": "GLKAIDAAIPVGLGQRELIIGDRQTGKTSIAIDTMLNQARSDVLCIYCAVGQRGDAVARVIRALKDGEMLQRCIVMSAGDEEAPGLSYIAPYAAMTMAEYFSAQGQDVLVIFDDMTHHARSYRELSLLLRRPPGREAFPGDIFYVHARLLERAGQFGADIGGGSITTLPVVETQAGNLSAYIPTNLISITDGQIYLSPQLVRKNQFPAVDLGLSVV"
  },
  {
    "reference": "MADILDSRPHAFSIKLWPPSLPTRKALIERITNNFSSKTIFTEKYGSLTKDQATENAKRIEDIAFSTANQQFEREPDGDGGSAVQLYAKECSKLILEVLKKGPVAKVAARELISEDSVSPRETFFDISKGKRAFIEAEEAEELLKPLKEPGNAYTKICFSNRSFGLGAARVAEPILASLKDQLKEVDLSDFVAGRPELEALEVMNIFSDALQGSILSSLNLSDNALGEKGVRAFGALLKSLSSLEELYLMNDGISKEAAQAVSELIPSTENLRVLHFHNNMTGDEGALAIAEVVKRSPLLENFRCSSTRVGSKGGIALSEALEHCTHMEKLDLRDNMFGTEAGVSLSKTLSSFKHMTELYLSYLNLEDEGAIAIVNALKESASPIEVLEMAGNDITVEAASAIAACVAAKQDLNKLNLSENELKDEGCVQIANCIEEGHSKLQYIDMSTNYIRRAGARALAHVVVKKEAFKLLNIDGNIISEEGIEELKEIFKKSPELLGALDENDPDGEEDDDDEEDEEDEENEGNGNGELESKLKNLEVNQED",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.2. The ideal protein would be one that plays a critical role in nucleocytoplasmic transport through GTPase activator activity within phragmoplast, nuclear membrane, nuclear envelope, spindle.3. Prioritize Acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "DPLVLAQQLTHIELDRVSSIYPEDLMQIVSHMDSLDNHRCRGDLTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQMANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFVASFR",
    "response#2": "DPLVLAQQLTHIELDRVSSIYPEDLMQIVSHMDSLDNHRCRGDLTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQMANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFVASFH",
    "response#3": "LSTGIPKEWRRKVWLTLADHYLHSIAIDWDKTMRFTFNERSNPDDDSMGIQIVKDLHRTGCSSYCGQEAEQDRVVLKRVLLAYARWNKTVGYCQGFNILAALILEVMEGNEGDALKIMIYLIDKVLPESYFVNNLRALSVDMAVFRDLLRMKLPELSQHLDTLQRTANKESGGGYEPPLTNVFTMQWFLTLFATCLPNQTVLKIWDSVFFEGSEIILRVSLAIWAKLGEQIECCGS"
  },
  {
    "reference": "MKYIVSSEEMKRYDKNTTKVYNVPEIVLVEQAAGAMVNELSNLLSKTNTKLNIEINKKPILVVCGIGNNGADGVAVARLLNEKGYEAVVCIITDKQIENSSESFRLELGIYKSYGYKVNDGSLVESLYSDYQNGIPCKYSVIIDGMFGVGLSRDIEGLYYDVIKRLNVLEALKISIDIPSGISSTTGAVLNIAFKADYTYTFSFSKLGHFLWPGVDYCGNVIICDIGITTKSFLDSPPEFRFLEKKDLELLPQRTRNTNKGSYGKVLVIAGNRNMAGAAIFSALAAYKMGCGLVRIFTNEINRNIIQSVLPEAVLVTYTQTEEIETLLQKNLSWADSVVLGPGLGVGETSKEIVKLVIANTDCPTVIDADALNIISYNDDIKEMIKEVTIVQNKIFIFTPHLGELSRLLKVDINEIKENIVNFSKKVADEYRVICVSKDAHTVVCDSCGINYLNLSGCEGLATAGSGDVLTGIIASLLGQKMDPNFATALGVYLHGLAGECASRDTTASVMARDIANAIKIILKEIRY",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be optimized for NADPHX epimerase activity.2. The K(+), (6S)-NADPHX, AMP binding site should be located in a region of the protein that is accessible to the ligand.3. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. The designed protein sequence is ",
    "response#1": "VVVIGGNKEYHGAPVLSARGALGTGIDMVYLFVPEVLLNPIRCISPDFIVTGYSGEKLTQISLDEGNKIFKKADAAVIGPGLGDDSETIEAVLSFLNHWDKPVVVDADALRALAFDKKILRGKHAILTPHAGELRRLSGLESENREATVKKAAEEFNATLLLKGSTDIVASPRGRIKFNMTGNAGMTRGGTGDVLAGIAAGLLAQEAEPFYAACASAFINGYSGDRLLERKGYFFLASELSMEIL",
    "response#2": "GVIAGSDDFPGAGVLTTMAAVCAGVGMVRYVGPRRPSDHVLTAVPEAVHGVGPVQAWVIGSGLAEDLSRLDPDQRAAIETALDSDVPVLIDAGGLDLLKELGLGVRRDRPTLLTPHAGECARLLDVPRAAVEADPIGHAERLAEQSGATVLIKGAVTVVVPGSSAAEGAPLLAQADGPPWLATAGTGDVLAGLAGALLAGGLPPAIAGALAALVHGVAADDAVARLLPVPGAESDKYRRGVVGIAAGSARYPGAAVLAVAGALRGGAGAVRYVGPAGHAVLARYPETLVSDQGPERAGRVQAWVVGPGAGDDAGTVAQVLAADVPVLIDADGLRLADVAAVRGRTAPTLMTPHAGEAAALLGVSREEVEAGRLAAVRELAARYGSAVLLKGSTTVVAGAGGGGAVRVNPTGTAWLATAGSGDVLSGLAGSLLAAGLSAVDAGSAGAYLHGLAGRFAAAGAPVGAHEVAEAVPGSR",
    "response#3": "MRQMDRQAIEHYGIFEDLLMENAGHAVYFMILEVFPVLEGKRFVVFCGLGNNGGDGLVVARKLHSNGARVNVFLLGDPEKYKGSARKNYEIVKGLSLPMKRLTKIGQAAYPLEHCDAVVDAMLGTGLSRPLEGLYRDVVLAINRAGKKVFSVDIPSGINGDTGDVMGVAVRADATATFGLPKLGNLLYPGFEYGGNLYVTHISFPPAIYADSNKGTFGKVLLVAGSVNMAGAAVLAAKAAYRSGCGLVRIYTPEENRIILQTAVPEAILTTYSAKKADTQQLIECLSWADVIILGPGIGKSDVAHSIVKTVLKNAAVPMVVDADALNIIAEQTEQLLRPHTELVLTPHLGEMSRLKQLAVSYIKENIEQVAEDFAREYNVICVLKDSRTVTAIPYGKTFINRNGNSGMATAGSGDVLSGIIGSLMAQGLSAEQAAPLGVYVHAAAADERSKTVSKHGLTAGEIL"
  },
  {
    "reference": "MSEISTKYIFVTGGVLSSLGKGITAASIGTLLKHTGQRVGVLKLDPYINVDPGTMSPLEHGEVFVTKDGAETDLDLGHYERFLDTSLTQNNNFTTGLVYKTVIENERKGKYLGKTIQVVPHIVDEIKERIIRAVEGKSILIVELGGTTGPIEGLPFLETIRQMKHELGKSKVMNIHVTLLPYIKAAGELKTKPTQHSVQELRRIGIAPHMLVLRAEVPVNADIKRKIAYSCDVDEESVIVAEDASTIYQVPLNFLRQNILSPVCKQLELDNCEPKMDEWTDLVHKIIMPSNEIKIAFVGKYLDLKESYKSLTEALIHAGAHLDAKVNIKWVDSEKIENDGAAKYLSDCDGVLVAGGFGERGVEGKILAIQYAREEKVPFLGICLGMQLSMIEFARNVLGLKEANSVEFKKDTSDAIIYLIDEFIDASGSKQVRTTTSPMGGTMRLGEYECETKEGSNLRAAYDTKVIFERHRHRYEANPKYKEDFESNGMDITGESHGLIEAVEIKDHPWFLGVQFHPEFTSRLQSANPSIMAFVNASMEHKTVDV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should contain one or more CTP synthase N-terminal domains that are essential for its function.2. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen3. The protein should be able to bind L-glutamine ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "LPDAYFSVAEALRHAGLHHGSTVEIRWINSEELESNPVEPYLDDVDGILVPGGFGDRGIEGKINSIRYAREQKVPYLGICLGMQLAVVEYSRNVLGWKAANSSEFDSATEYPVIDLLPEQKKLDKMGGTMRLGQYSCKILPETMAHRAYGQDEINERHRHRYELNNQYRKELSESGLTISGTLPDGYLVEIVELPGHPWYVATQFHPEFKSRPNRPHPLFRDFIEAALAY",
    "response#2": "VTIAVVGKYVDHKDAYKSVGEALKHGGLRQRTKVNLKWLESQDVEADAGVLLGDVDGILVPGGFGDRGFEGKVLTAQYAREHGVPYFGICYGMQAAVVDYARHVAGLEGANSTENDKQCLHPVIGLITEWRTNTGEIEKRDEKSDLGGTMRLGLQEQRLKPGTLVRELYGKDVVAERHRHRYEFNNRYRTQLEDAGLVIAAKSMDDLLVEMVELPRAVHPWFLACQAHPEFLSTPRDGHPLFVGFR",
    "response#3": "LPDAYKSINEAFVHAGAKNECKVRLKHIHSEGLTPENAAEKLAGMHGVLVAPGFGSRGIEGKIEAIRYVRENNIPFFGICLGMQCAVIEYARNVIGWKDAHSTEMDDHTKHPVISMMEEQKKIKNKGGTMRLGAYACDLKKGSKAASIYGKAHISERHRHRYEFNSKYLKEFEDAGLIASGINPENNLVEIVELKNHPFFIGGQFHPELKSTVANPHPLFVNFVY"
  },
  {
    "reference": "MENRWQVMIVWQVDRMRIRTWHSLVKHHMYISRKAKNWSYRPHYASPHPRVSSEVHIPLGDARLVITTYWGLHPGERDWQLGHGVSIEWRQKKYNTQVDPDLADQLIHLYYFDCFSESAIRSAILGRIVSPSCEYQAGHNKVGSLQYLALAALIKPKKIKPPLPSVRKLTEDRWNKPQKTKGHKGSQTMNGH",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.2. For general function, the protein need meet that Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G; Recruits an active E3 ubiquitin ligase complex composed of elongin BC, CUL5, and RBX2 to induce polyubiquitination of APOBEC3G and APOBEC3F; Finally, Vif also contributes to the G2 cell cycle arrest observed in HIV infected cells.; Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism; In turn, they are directed to the 26S proteasome for degradation; Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions3. The protein's functional design necessitates the inclusion of a HCCH motif motif.4. The protein can be targeted to cytoplasm, host cell plasma membrane and perform RNA binding. The designed protein sequence is ",
    "response#1": "MKVKEIRKNYQHLWRWGTMLLGMLMICSAADQWXVGXTTXGXTXXXNXTXGXGXGXVXEXXXNAXGXDXNXIXHXGMXWDXXXIXIXKXXXCXIXKXPXXXRXAXAXIVXXXXXXXXXXXXXXXXXXXXXXKXXXIXIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDXSXXGXRXVQXXXPXFXAXXXXXXXXXXXXXXX",
    "response#2": "MENRWQVMIVWQVDRMRIRTWNSLVKHHMYVSKKAKDWFYRHHYESNHPKISSEVHIPLGDAKLVVTTYWGLHTGERDWHLGQGVSIEWRLKKYSTQIDPDLADKLIHLYYFDCFSESAIRKAILGHRVSPRCEYQAGHNKVGSLQYLALTALITPKKIKPPLPSVRKLVEDRWNKPQKTRGRRGNHTMNGH",
    "response#3": "MENRWQVMIVWQVDRQKVKTWNSLVKHHMYISKKAKRWSYRHHYESTNPRVSSEVHIPLGEAKLVITTYWGLHTGERDWHLGQGVSIEWRERRYSTQVDPDLADQLIHLHYFDCFSDSAIRKAILGYRVSPRCEYPAGHKKVGSLQYLALTALIKPKRIKPPLPSVRKLVEDRWNKPQKTRGRRGNHTMNGH"
  },
  {
    "reference": "MPSTKYIFVSGGVTSSLGKGIISSSLAKLLQARGYSVTIQKLDPYINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNIPTSQDNNVTTGLVYQSVINKERRGDYLGKTVQIIPHITDEIKRRIKLAAKKHKFDIVITEIGGTVGDIESLPYVESVRQMKWEMGSSNCIVIHLTLVPYLSTSGELKTKPTQHSVKVLLENGIQPDILVLRTRKKLTEEVRKKVSLFCNVDISSVIESIDVSTIYEVPLLMQKEKLDITVLKKLNLPVRQAPGLKPWKTFLGKLKNPKSSVKIGLIGKYVELQDAYKSITESFIHAGAMNECKVDLIPIHSESITEDNVEEKLGNLKGILVAPGFGHRGIEGKIIAVKLARENNVPYLGICLGMQCAVIEFARNILNLKGADSTEMDIKPKHPVIDLMEEQKGVTEKGGTMRLGAYACRLKKGSFSYLAYKKDMVRERHRHRYEFNNKYMHDFDNAGMKPVGINPETNLVEIMEIPEHKWFVGVQFHPEYKSTVLNPHPLFVDFVKASKG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The Nucleophile, active site, Nucleophile; for glutamine hydrolysis active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The designed protein should interact with enzymes and substrates within the CTP from UDP: step 2/2 pathway to enhance or regulate its activity.3. The presence of CTP synthase N-terminal domains is critical for the interaction of the designed protein with its intended targets.4. The protein design must be able to perform CTP synthase activity, ATP binding in order to contribute to glutamine metabolic process. The designed protein sequence is ",
    "response#1": "VHIAICGKYTQLQDAYKSIVEAFVHAGVVNNTAVKLKWINSEKLTSENVETHLSGVDGILVPYGFDTRGVEGKLLAVKYARENKIPFFGICLGLQCAVIEFARNVCGISEANSAEFDAKTPHPVIHIMESQLGIKQKGGTMRLGSYPCVTKENTLARKIYGSEIIIERHRHRYEVNNDYRRTLEEHGMVLSGLSPDGTLVEIIELKEHPFFIGCQFHPELKSRIMRPHPIFVHFY",
    "response#2": "LPDAYKSVVEALSAAGFAHRAKINLRWVESDDCESADGTAKALAGVDAVVIPGGFGNRGVPGKIGALTYTRTRGIPTLGLCLGMQCMAIEGLREIAGLVGADSQEFDAAAEHPVISTMADQTDIVAGRGDMGGTMRLGSYEAKLAEGSIAAELFGTTEVTERHRHRYEVNNAYREQLTKSGFVISGLSPDGRLVEFIELDRDVHPYYVGTQAHPELKSRPTRPHPLFDGLLAACLQT",
    "response#3": "LPDAYKSVVEALSAAGFAHRAKINLRWVESDDCESADGTAKALAGVDAVVIPGGFGNRGVPGKIGALTYTRTRGIPTLGLCLGMQCMAIEGLREIAGLVGADSQEFDAAAEHPVISTMADQTDIVAGRGDMGGTMRLGSYEAKLAEGSIAAELFGTTEVTERHRHRYEVNNAYREQLTKSGFVISGLSPDGRLVEFIELDRDVHPYYVGTQAHPELKSRPTRPHPLFDGLLAGVLKA"
  },
  {
    "reference": "MLAVHFGAGNIGRGFIGQLLQKAGYEIAFVDVNAALVDELNERKMYRVQLATTGKPESVVEGVRAINGQDVAAVAEAIATADLVTTAVGPNILPHIAGAIAAGITQRLTVHARPLNVIACENMIGGSEKLKEHVYEHLTESVQAQAAQWIGFPNAAVDRIVPLQQHADKLLVMVEPFFEWVVDSSQIVGEVPAIEGVTYVEDLAPYIERKLFTVNTGHAVIAYLGYQLGMKTIDEAMRDDRIVQATRGALQETGALLAAKYGFDPQVHGQYVEKILGRYTNPLLSDDIVRVARSPIRKLSQHDRLVGPALQCMEKGMNASYLGLAIAAALAFDYPEDAESARIQASLKEFGWENTLHNCTGIPAGHPLEEIVREQARRLKEWSGSH",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein with mannitol-1-phosphate 5-dehydrogenase activity is needed to enhance mannitol metabolic process in a specific context.2. The protein should have a high affinity for NAD(+). The designed protein sequence is ",
    "response#1": "GLGHMAVKFGKAFGAKVTVISTSPNKKDEAVNRLGADSFVVSREPEQMQSAMGTLDGIIDTVSAAHPLLPLLGLLKSQGKMIMVGVPDKPLELPVFPLLQGRKILAGSCIGGMKETQEMIDFAR",
    "response#2": "GLGHMAVKFGKAFGAKVTVISTSPNKKDEAVNRLGADSFVVSREPEQMQSAMGTLDGIIDTVSAAHPLLPLLGLLKSQGKMIMVGVPDKPLELPVFPLLQGRKILAGSCIGGMKETQEMIDFAQ",
    "response#3": "GLGHMAVKFGKAFGAKVTVISTSPNKKDEAVNRLGADSFVVSREPEQMQSAMGTLDGIIDTVSAAHPLLPLLGLLKSQGKMIMVGVPDKPLELPVFPLLQGRKILAGSCIGGMKETQEMIDFAR"
  },
  {
    "reference": "MSSSFFIPFLVSQILLSLFFSIIILIKKLLRTQITVGTHYYISVISLLALIAPFIPFHFLKSHHFDWILNLGGAQSALSQTHSTDKTTEAIGQHVNWVQDFSLSIEQSSSKMIDSAFFAVWILGVAVMLLATLYSNLKIGKIKKNLQIVNNKELLSLFHTCKEEIRFHQKVILSRSPLIKSPITFGVIRPYIILPKDISMFSADEMKCVLLHELYHCKRKDMLINYFLCLLKIVYWFNPLVWYLSKEAKTEMEISCDFAVLKTLDKKLHLKYGEVILKFTSIKQRTSSLLAASEFSSSYKHIKRRIVTVVNFQTASPLLKAKSALVFTLVLGAILAGTPSVSILAMQKETRFLPGTNVEYEDYSTFFDKFSASGGFVLFNSNRKKYTIYNRKESTSRFAPASTYKVFSALLALESGIITKNDSHMTWDGTQYPYKEWNQDQDLFSAMSSSTTWYFQKLDRQIGEDHLRHYLKSIHYGNEDFSVPADYWLDGSLQISPLEQVNILKKFYDNEFDFKQSNIETVKDSIRLEESNGRVLSGKTGTSVINGELHAGWFIGYVETADNTFFFAVHIQGEKRAAGSSAAEIALSILDKKGIYPSVSR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein's architecture should predominantly feature strand-helix-turn-strand-turn-strand-helix-helix-helix-turn-helix-helix-helix-turn-strand.2. For general function, the protein need meet that Mechanistically, activation of the signal transducer involves acylation of a serine in the C-terminal sensor domain upon binding of the beta-lactam antibiotic; Integral membrane protein involved in sensing of the presence of beta-lactam antibiotics and transduction of the information to the cytoplasm3. The protein should be optimized for plasma membrane-specific folding and stability, while maintaining penicillin binding.4. The protein should have a Helical domain that is long enough to span the entire membrane.5. The Extracellular topological domain of the protein should be designed to be flexible or rigid.6. The Acyl-ester intermediate active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "ETGECARRVSPCSSFKLPLAIMGFDSGILQSPKSPTWELKPEYNPSPRDRTYKQVYPALWQSDSVVWFSQQLTSRLGVDRFTEYVKKFEYGNQDVSGDSGKHNGLTQSWLMSSLTISPKEQIQFLLRFVAHKLPVSEAAYDMAYATIPQYQAAEGWAVHGKSGSGWLRDNNGKINESRPQGWFVGWAEKNGRQVVFARFKRYKHIVPNDEPKVGVIYGLAVLGAGGIGDVTKIIVQILESKNPGTHLLNISGDIAKHSITLASALSKKLVAEKKLPLPKKDIDLNNKEIYIQFSQSYSKIDGDSATAAVCLAIISALLDIPLKQDFAITGSLDLSGNVLAIGGVNEKIEAAKRYGFKRVIIPEANMIDVIETEGIEIIPVKTLDEIVFKRYKHIVPNDEPKVGVIYGLAVLGAGGIGDVTKIIVQILESKNPGTHLLNISGDIAKHSITLASALSKKLVAEKKLPLPKKDIDLNNKEIYIQFSQSYSKIDGDSATAAVCLAIISALLDIPLKQDFAITGSLDLSGNVLAIGGVNEKIEAAKRYGFKRVIIPEANMIDVIETEGIEIIPVKTLDEIVEPKVGVIYGLAVLGAGGIGDVTKIIVQILESKNPGTHLLNISGDIAKHSITLASALSKKLVAEKKLPLPKKDIDLNNKEIYIQFSQSYSKIDGDSATAAVCLAIISALLDIPLKQDFAITGSLDLSGNVLAIGGVNEKIEAAKRYGFKRVIIPEANMIDVIETEGIEIIPVKTLDEIVPLVFDEPKVGVIYGLAVLGAGGIGDVTKIIVQILESKNPGTHLLNISGDIAKHSITLASALSKKLVAEKKLPLPKKDIDLNNKEIYIQFSQSYSKIDGDSATAAVCLAIISALLDIPLKQDFAITGSLDLSGNVLAIGGVNEKIEAAKRYGFKRVIIPEANMIDVIETEGIEIIPVKTLDEIVPLVFD",
    "response#2": "GSLVIMDVKTGEILAMTNQPTYNPNNRRNLQPAAMRNRAMIDVFEPGSTVKPFSMSAALASGRWKPSDIVDVYPGTLQIGRYTIRDVSRNSRQLDLTGILIKSSNVGISKIAFDIGAESIYSVMQQVGLGQDTGLGFPGERVGNLPNHRKWPKAETATLAYGYGLSVTAIQLAHAYAALANDGKSVPLSMTRVDRVPDGVQVISPEVASTVQGMLQQVVEAQGGVFRAQVPGYHAAGKSGTARKVSVGTKGYRENAYRSLFAGFAPATDPRIAMVVVIDEPSKAGYFGGLVSAPVFSKVVHRTYFQMEQQIPAHRGLITDRNGEPLAVSTPVTTLWANPKELMTAKERWPQLAAALGQDTKLFADRIEQNAEREFIYLVRGLTPEQGEGVIALKVPGVYSIEEFRRFYPAGEVVAHAVGFTDVDDRGREGIELAFDEWLAGVPGKRQVLKDRRGRVIKEPKVGVIYGLAVLGAGGIGDVTKIIVQILESKNPGTHLLNISGDIAKHSITLASALSKKLVAEKKLPLPKKDIDLNNKEIYIQFSQSYSKIDGDSATAAVCLAIISALLDIPLKQDFAITGSLDLSGNVLAIGGVNEKIEAAKRYGFKRVIIPEANMIDVIETEGIEIIPVKTLDEIVPLVFDFKRYKHIVPNDEPKVGVIYGLAVLGAGGIGDVTKIIVQILESKNPGTHLLNISGDIAKHSITLASALSKKLVAEKKLPLPKKDIDLNNKEIYIQFSQSYSKIDGDSATAAVCLAIISALLDIPLKQDFAITGSLDLSGNVLAIGGVNEKIEAAKRYGFKRVIIPEANMIDVIETEGIEIIPVKTLDEIV",
    "response#3": "FKRYKHIVPNDEPKVGVIYGLAVLGAGGIGDVTKIIVQILESKNPGTHLLNISGDIAKHSITLASALSKKLVAEKKLPLPKKDIDLNNKEIYIQFSQSYSKIDGDSATAAVCLAIISALLDIPLKQDFAITGSLDLSGNVLAIGGVNEKIEAAKRYGFKRVIIPEANMIDVIETEGIEIIPVKTLDEIVETGECARRVSPCSSFKLPLAIMGFDSGILQSPKSPTWELKPEYNPSPRDRTYKQVYPALWQSDSVVWFSQQLTSRLGVDRFTEYVKKFEYGNQDVSGDSGKHNGLTQSWLMSSLTISPKEQIQFLLRFVAHKLPVSEAAYDMAYATIPQYQAAEGWAVHGKSGSGWLRDNNGKINESRPQGWFVGWAEKNGRQVVFAREPKVGVIYGLAVLGAGGIGDVTKIIVQILESKNPGTHLLNISGDIAKHSITLASALSKKLVAEKKLPLPKKDIDLNNKEIYIQFSQSYSKIDGDSATAAVCLAIISALLDIPLKQDFAITGSLDLSGNVLAIGGVNEKIEAAKRYGFKRVIIPEANMIDVIETEGIEIIPVKTLDEIVPLVFDFKRYKHIVPNDEPKVGVIYGLAVLGAGGIGDVTKIIVQILESKNPGTHLLNISGDIAKHSITLASALSKKLVAEKKLPLPKKDIDLNNKEIYIQFSQSYSKIDGDSATAAVCLAIISALLDIPLKQDFAITGSLDLSGNVLAIGGVNEKIEAAKRYGFKRVIIPEANMIDVIETEGIEIIPVKTLDEIVS"
  },
  {
    "reference": "MIELINRSGLRVISADINSGINGENGLGPVAVRSDLTVSIGSLKPGHFLGRAKDVMKQVVNADIGIPPVGHSLSLMEEEDVKALLPRRDHFSNKGTYGTVVLIGGSLPYSGAIRLAATANAAMRSGAGIARIAAPRSLCPVVASSILESTLCPMEDREGSMLFSEESLARAVSGTRVAAFGMGAGDTEETRKMVDWLLQNYEGILILDADGLNALAAIGPDRLQSAVCRVVLTPHPGEFARLNCCTVRDVLEAPAAMAEDFARKQGVIILLKGSATLITDGENTRLTDRGCPGMATAGSGDVLSGILAALCAWLPSNLLQATAAAAWINGRAGELAQEKSSDISMTAGDTARALPEVFRLLRDQR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for AMP, (6S)-NADPHX binding.2. For general function, the protein need meet that Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP; Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.3. A protein with NADHX epimerase activity, NADPHX epimerase activity, kinase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity is needed to enhance phosphorylation, nicotinamide nucleotide metabolic process in a specific context.4. The protein should be designed to have high stability and activity towards (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.5. The presence of YjeF N-terminal domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "MRQLDRQAIEEFGISEELLMENAGLASFQVIEEEIGVEGVKFLVLCGGGNNGGDGFVVARKLHSAGGQVEVFVLGEEKKYRGAAQKNYEIATKLGFPLKKLESIEELQEALSRCEVVVDALFGTGLDRAVEGIYREVIQRVNEADKIVVSLDIPSGISGNNGLEMGEAIWADYTITFGLPKLGNLLYPGYGHGGFLFVTHISFPPQLTPAAEKYTRGVVGVATGSATYPGAAVLSVGGAVAGPTGLVRYAGSAAAQVLDRYPSVIATGRVADAGRVQAWVCGSGLGGGEHAVTELRSVLAAPVPVVLDADALTMLVDGSWAEQLRGRQAPTVITPHDREFARLAGQPPGDDRVGAALRLAAWMRATVLLKGDRTVVAGPDGRAYVNATGTPALATGGTGDVLAGLLGSLLAAGLDPQRAALAAAYVHGLAGRAAARSGPVTATDDDVADLLPRPEAESDKYRRGVLGIVAGSDRFTGAAVLAVGGAIRAGAGLVRFASAETAVRQVRNRWPEAITTVLDGPSVAEVGRVQAWVLGPGMGTGEEAVALARAVLATDVPVLVDADGLTILANDRSLLRRSAPTVLTPHAGELSRLLDVPRERIEAARMEHVRRAVAELGAIVLLKGSTTLIAEQDRPVRVNTTGTPWLATGGTGDVLSGIAGTLLSGGLSGYDAVSCAAHLHGLAAQAASTGISGGHGPIAAMDVATMLPQVIEALA",
    "response#2": "MQRADQNALREGISLRSLIDEAGRAVAAEISSRFSNAKRVAVLVGPGNNGRDALVAARYLREEKRSADLFGTANGHDEIAPLQAFHGGSYDLVLDGLWGAGLSRDVEGDAEDALRKAEDSGRPIVSIDIPSGVDGGNGQIRGYAPKVDLTVTFVRRKPGHCLLPGRGHCGTIVVAPVGLSETAMAESDKYRRGVLGIVAGSDRFTGAAVLAVGGAIRAGAGLVRFASAETAVRQVRNRWPEAITTVLDGPSVAEVGRVQAWVLGPGMGTGEEAVALARAVLATDVPVLVDADGLTILANDRSLLRRSAPTVLTPHAGELSRLLDVPRERIEAARMEHVRRAVAELGAIVLLKGSTTLIAEQDRPVRVNTTGTPWLATGGTGDVLSGIAGTLLSGGLSGYDAVSCAAHLHGLAAQAASTGISGGHGVSAWWPSLAPAAEKYTRGVVGVATGSATYPGAAVLSVGGAVAGPTGLVRYAGSAAAQVLDRYPSVIATGRVADAGRVQAWVCGSGLGGGEHAVTELRSVLAAPVPVVLDADALTMLVDGSWAEQLRGRQAPTVITPHDREFARLAGQPPGDDRVGAALRLAAWMRATVLLKGDRTVVAGPDGRAYVNATGTPALATGGTGDVLAGLLGSLLAAGLDPQRAALAAAYVHGLAGRAAARSGPVTAVEVAAALRPL",
    "response#3": "MKKAEAMCEQKGTSCAVLMRNVGSAIALHISRIVKPCRAAVLVGSGNNGGDGFAVAHNLRKRGFSPLIVLVGSAPKTDLAIDCFNEYKPDYEAVLSYPDQPETVLSELGSCGIIIDCVYGTGFHGELAPPVRRLFSYCNGSAALRFCADIASGCNATDGNADEYSFRADMTFALGAVKTGQLYVPCSEFSGDIVLLDIGISEACFTDDDVADLLPRPEAESDKYRRGVLGIVAGSDRFTGAAVLAVGGAIRAGAGLVRFASAETAVRQVRNRWPEAITTVLDGPSVAEVGRVQAWVLGPGMGTGEEAVALARAVLATDVPVLVDADGLTILANDRSLLRRSAPTVLTPHAGELSRLLDVPRERIEAARMEHVRRAVAELGAIVLLKGSTTLIAEQDRPVRVNTTGTPWLATGGTGDVLSGIAGTLLSGGLSGYDAVSCAAHLHGLAAQAASTGISGGHGPIAAMDVATMLPQVIPESDKYRRGVVGVAAGSERYPGAAVLAVAGALRGGAGAVRYVGPPAEAVLARFPEVLIGPGRVQAWVAGSGLGDAPEARERLARALDDGVSTVVDADGLRMLGPAPGRTARGPLVLTPHAGEAAALLGVAREEVEEARLESVRQLARQTGATVLLKGSTTLIASPPGEADAGEEDGGTVLANLNGTGWLATAGTGDVLAGLIGSLLAAGLAPADAAAVGAHLHGLATDDDVADLLPRPEAESDKYRRGVLGIVAGSDRFTGAAVLAVGGAIRAGAGLVRFASAETAVRQVRNRWPEAITTVLDGPSVAEVGRVQAWVLGPGMGTGEEAVALARAVLATDVPVLVDADGLTILANDRSLLRRSAPTVLTPHAGELSRLLDVPRERIEAARMEHVRRAVAELGAIVLLKGSTTLIAEQDRPVRVNTTGTPWLATGGTGDVLSGIAGTLLSGGLSGYDAVSCAAHLHGLAAQAASTGISGGHGPIAAMDVATMLPQVI"
  },
  {
    "reference": "MTDTLHALRPERVPGVPLSRLAPGSPEVPVTGVTLDSRTVLPGDLYVALPGAHVHGAQFVDAATAAGAIAVLTDARGGELAGHATIPVLVTSDPRHAMATAAAEVYGHPGDAMALFGLTGTNGKTSTTFLLQAALEAIDVRVGTIGTLGFWVGKTALEGSRSTITTPEAPDLQALLALMRERGAHAVAIEVSSVALDRGRVDELTFAVSGFTMFGQDHLDFHGDLESYYRAKTRLFLDGRSRAAVVNIDDPAGMRLAGEIRRDASARLTTTGTAPEAEYRLLETSPREDGSWCAQVATPQGRLDFVVGMLGDFNVRNALTALAMVGESGGDVAHAAKGLAHAAVPGRMQRLALAGDSPNVLIDFAHTPQAVSAALAALPPGRRIAVLGCGGDRDATKRGPMGAAAARGADVVIVTDDNPRSETPLAIREAVLAGALAEPAARARSIRDGGDRRGAIESALREARPGDWVAILGKGHERGQTIGTTIHAFEDATVVRELWSTIQGA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.2. The UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP binding site should be located in a region of the protein that is accessible to the ligand.3. The protein design should take into account the physiological conditions of the peptidoglycan biosynthesis, Cell wall biogenesis pathway.4. The designed protein should have ATP binding to facilitate cell cycle.5. The protein should have a fast turnover rate for ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "MKLSITAVVGDSRLAVRGSLFVAIRGMQQDGAKFVDSAVEKGAIAVVSDHPLATRRSPPAWVEVEDARSALALIAANFYGHPTSEMRVIGITGTKGKTTTTYMIKSILEEVGITGTKGKTTTTYMIKSILEEVGHKVGLIGTIEAIIGNKKIPASNTTPESFTIHQYFAKMAEDGCDCVVMEVSSQGLMLNRTAGIPFEIGIFTNLGRDHIGPNEHKDFEDYKRCKGLLFKQCKLGIANVDDPYFKEVFSGATCRTETFGFSERADLRAVNTHLMSRPGYLGVSYHVTGLLDFDVEIDIPGKFSVYNSLTAIAVGICKTLGFDLLRISTAIESVKGVPGRFEYVESAGGPTVIVDYAHKPDALEKLLHAVRDLAAKKRLVLVFGCGGDRDKDKRPMMGDIAGRLADFTVLTSDNPRSENPDFIIDDIEKGIRRGTYVRMTDRREAIARTIDEASDDDVIVIAGKGHETYQIVGTKKYDFDDRVEARKAIDALKK",
    "response#2": "MKLRDLLAPFAPPPPEGALVTGITNRSGEVRPGDLYAAVPGTRTHGAKFAAEAQAAGAVCVLTDAEGSDAVAASHVTLPIIVVPDVKAVLGEASARVTGTNGKTTIATLLWKLFTALGYKCGLISTVQNQVGDKVFAATHTTPDAVSINALLKQMLDEGCVYVFMECSSHAIHQHRITGLQFTGALFTNITHDHLDYHKTFDEYIRVKKSWFDKLPSSAFAISNADDKRGAVMLQNTHARKYFYSLKTMAEYKGKILDNGLEGLHMTINDKEVYFRLIGEFNAYNLLAVYGAALLRLGVPLPETARALAEVVPVPGRMDSIAVPGRPLVVVDYAHTPDALEKVLGALRPLASGRSGQLWCVFGCGGERDAGKRPLMAAAAEKGSDRVVVTSDNPRSENPDAIISQILQGFARRDAVQVQADRARAIAHAVGQAQAGDVVLIAGKGHETYQEIGTVKRPFDDREEAVAALAARRAA",
    "response#3": "MGLSQLLAPWLQAQVLDADDSRRLEAGDVFVAYPGERVDGRTHIPQAIKQGAAAVLWERVDGRWLDDWHVPNLAVEGLREQVSEIAGEFYGRPSDELTLVGITGTKGKTTTTYMIKSILEEVGHKVGLIGTIEAIIGNKKIPASNTTPESFTIHQYFAKMAEDGCDCVVMEVSSQGLMLNRTAGIPFEIGIFTNLGRDHIGPNEHKDFEDYKRCKGLLFKQCKLGIANVDDPYFKEVFSGATCRTETFGFSERADLRAVNTHLMSRPGYLGVSYHVTGLLDFDVEIDIPGKFSVYNSLTAIAVAHTMGVASETIQAGLATLAGVPGRMDSIAVPGRPLVVVDYAHTPDALEKVLGALRPLASGRSGQLWCVFGCGGERDAGKRPLMAAAAEKGSDRVVVTSDNPRSENPDAIISQILQGFARRDAVQVQADRARAIAHAVGQAQAGDVVLIAGKGHEAYQEINGVRHPFDDRTVALQALAAR"
  },
  {
    "reference": "MASLGGLQNVSGINFLIKEGPKVNAKFELKPPPYPLNGLEPVMSQQTLEFHWGKHHKTYVENLKKQVVGTELDGKSLEEIIVTSYNKGDILPAFNNAAQVWNHDFFWECMKPGGGGKPSGELLELIERDFGSFVKFLDEFKAAAATQFGSGWAWLAYRARKFDGENVANPPSPDEDNKLVVLKSPNAVNPLVWGGYYPLLTIDVWEHAYYLDFQNRRPDYISVFMDKLVSWDAVSSRLEQAKALITSA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The stability of the protein-Fe cation complex should be optimized to maximize enzymatic activity.2. The desired protein should be able to perform 2 H(+) + 2 superoxide = H2O2 + O23. The protein should have superoxide dismutase activity and be active in chloroplast nucleoid. The designed protein sequence is ",
    "response#1": "TVDAAKEKHVPVIEKTNDGIIVKVGSVAHPMEEKHFIQWIELIANGKAYRQHLSPGDKPEACFPLITGPLKVREYCNLHGLWSSC",
    "response#2": "PTGELAAAIADAFGSFDKFRAQFHAAATTVQGSGWAALGWDTLGNKLLIFQVYDHQTNFPLGIVPLLLLDMWEHAFYLQYKNVKVDFAKAFWNVVNWADVQSC",
    "response#3": "VDAAKEKHVPVIEKTNDGIIVKVGSVAHPMEEKHFIQWIELIANGKAYRQHLSPGDKPEACFPLITGPLKVREYCNLHGLWSA"
  },
  {
    "reference": "MADRLIVKGAREHNLRSVDLDLPRDALIVFTGLSGSGKSSLAFDTIFAEGQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTNRNPRSTVGTITEVYDYLRLLYARAGTPHCPTCGERVARQTPQQIVDQVLAMPEGTRFLVLAPVVRTRKGEFADLFDKLNAQGYSRVRVDGVVHPLTDPPKLKKQEKHDIEVVVDRLTVKAAAKRRLTDSVETALNLADGIVVLEFVDHELGAPHREQRFSEKLACPNGHALAVDDLEPRSFSFNSPYGACPECSGLGIRKEVDPELVVPDPDRTLAQGAVAPWSNGHTAEYFTRMMAGLGEALGFDVDTPWRKLPAKARKAILEGADEQVHVRYRNRYGRTRSYYADFEGVLAFLQRKMSQTESEQMKERYEGFMRDVPCPVCAGTRLKPEILAVTLAGESKGEHGAKSIAEVCELSIADCADFLNALTLGPREQAIAGQVLKEIRSRLGFLLDVGLEYLSLSRAAATLSGGEAQRIRLATQIGSGLVGVLYVLDEPSIGLHQRDNRRLIETLTRLRDLGNTLIVVEHDEDTIEHADWIVDIGPGAGEHGGRIVHSGPYDELLRNKDSITGAYLSGRESIEIPAIRRSVDPRRQLTVVGAREHNLRGIDVSFPLGVLTSVTGVSGSGKSTLVNDILAAVLANRLNGARQVPGRHTRVTGLDYLDKLVRVDQSPIGRTPRSNPATYTGVFDKIRTLFAATTEAKVRGYQPGRFSFNVKGGRCEACTGDGTIKIEMNF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate; UvrA is an ATPase and a DNA-binding protein2. A protein that contributes to cellular response to DNA damage stimulus, negative regulation of strand invasion by functioning in plasma membrane with ATP hydrolysis activity, DNA binding, ATP binding.3. The ATP binding site should be located in a region of the protein that is accessible to the ligand.4. The protein's architecture should predominantly feature strand-strand-strand-strand-helix-turn-helix-helix-strand-strand-helix-helix-strand-helix-strand.5. The presence of ABC transporter domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "LVVDRLILETRNEHRILDSLELAMREGKGFVEIRNVDTGESKIFSENLMCPVCGIGFPEITPKLFSFNSPYGACPNCHGLGFTFEVDPSLVIDEEKSVLEGAIIPYRWDRRLSRWVAREIEKRGVSPHLPFKDLPEDVKEFILYGDDRFEGVVPKVQRWHRETESPEMKEWLEKNFIVQRTCSVCGGRRLNREALSVKINGLNIHEFTELSISEELEFLKNLNLTEREREIVGELLKEIEKRLEFLVDVGLEYLTLSRSATTLSGGESQRIRLATQIGSGLTGVIYVLDEPTIGLHPRDTERLIKTLKKLRDLGNTVIVVEHDEEVIRNADHIIDIGPGGGTNGGRVVFQGTVDELLKNPDSSLTGEYNRTYRVNRRFEGVIPRMERIYMETKSNYMRTYIGRFMSNHACPVCGGSRLRPESLSVTINGRSIHDVVEMSIREAHEFFDSLKLTEREEYIAREVLKEIRERLRFLIDVGLDYLTLSRSSGTLSGGEAQRIRLATQIGSGLVGVLYILDEPSIGLHQRDNRRLIETLKRLRDLGNTLIVVEHDEETILSADHVVDIGPGAGEHGGCVVAEGTPEEIMEDPDSLTGAYLE",
    "response#2": "LVVDRLILETRNEHRILDSLELAMREGKGFVEIRNVDTGESKIFSENLMCPVCGIGFPEITPKLFSFNSPYGACPNCHGLGFTFEVDPSLVIDEEKSVLEGAIIPYRWDRRLSRWVAREIEKRGVSPHLPFKDLPEDVKEFILYGDDRFEGVVPKVQRWHRETESPEMKEWLEKNFIVQRTCSVCGGRRLNREALSVKINGLNIHEFTELSISEELEFLKNLNLTEREREIVGELLKEIEKRLEFLVDVGLEYLTLSRSATTLSGGESQRIRLATQIGSGLTGVIYVLDEPTIGLHPRDTERLIKTLKKLRDLGNTVIVVEHDEEVIRNADHIIDIGPGGGTNGGRVVFQGTVDELLKNPDSSLTGEYTG",
    "response#3": "DRGDVVIRKHPFEGILNNMARRYKETESMSVREELAKNISNRPCIDCGGSRLRPEARNVYIGRTNLPIIAEKSIGETLEFFTALSLTGQKAQIAEKILKEIRERLQFLVNVGLNYLSLSRSAETLSGGEAQRIRLASQIGAGLVGVMYVLDEPSIGLHQRDNERLLNTLIHLRNLGNTVIVVEHDEDAIRAADHIIDIGPGAGVHGGQVIAQGNADEIMLNPNSITGKFLNL"
  },
  {
    "reference": "MKNIRNSSLQDLEALFKEWGESKFRAKQVYEWIWQKHVGDFSQMTNLSKALRDKLSEQFTIPKVRVHHSQFSSDGTIKNRLQLHDGHFVESVLIPTEKRMTVCVSSQVGCSLACKFCATGFLPRKRNMDFDEIVDEVTLANEQSLQHYGKPVTNIVFMGMGEPLLNYKNVLKAIDKITSPDCLGMSPRRITVSTAGISKMIRQLGDDGVRFKLALSLHAANDTKRDKIMPINETSNLDELVDALNYFYQKTKNEITFEYILFKNFNDSAKDADELVKLYRRVPVDLVNIIEYNTIDKADFTKPEDDVTESFMRYLESKRVNARLRRSRGKDIDAACGQLANVDHEVSQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be involved in rRNA base methylation by localizing to cytoplasm and exhibiting 4 iron, 4 sulfur cluster binding, rRNA (adenine-C2-)-methyltransferase activity, tRNA (adenine-C2-)-methyltransferase activity, tRNA binding, metal ion binding.2. The designed protein must have at least one Radical SAM core domain.3. The protein should have a low Km value for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.4. The [4Fe-4S] cluster binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MTSSELKELAVGMGEKPFRGAQLADWIYRKAVLNYEEMTNLPAAFIARLQDEFEITPSQVGCPMGCTFCATGLGGFTRNLSAGEIVGQYLALQSLSPRRVSHVVFMGMGEPLLNYDNLLAAIRLLHEEVKLSLRHITVSTVGLVTGIERLAKEEMPIHLALSLHSPLDDVRDRLMPVNQKWPVQVVVQAMRDYHAITGRKVTFEYLLIHEVNDPRRHLLDLSLAELRDWVVERGEKPFRAAQVWRWIYQGRAADFESMTDLSRSLRQHLAEDFVLFAGSIARHQTSPDGTEKLLIGLADGGEVECVLLRDGPRRSICVSSQVGCAMGCVFCASGLDGVDRNLTRGEILEQMLRLQHRLDHEERLSHIVMMGMGEPLANLERVLSALDVARSPEGLGISPRRITISTVGLPPAIDRLARHGVPYNLAVSLHAPEDELRNRLVPVNRKIGVEALMAAADRYFEASGRRLTFEYVLLGGLNDSPTQAAQLGHLLRGRGAMLNVIPYNPVAGLPYRTPTSTATSRFREVLERGGINVNFRVRKGGEIDAACGQLRRNRQATQQNFRRD",
    "response#2": "KKPPPHLADLDPAGRKALLEDNGLPGFRAKQLSTHYFARLVDAPEQMTDLPAAQRGELVSTLLPELMTPVRTSEADRGTTRKTLWRLFDGALVESVLMRYTDRTTMCVSSQAGCGMACPFCATGQGGLQRNMSTAEIVEQVMAGARSLARGEVPGGPGRVNNVVFMGMGEPLANYKAVVGAVRRLTDPAPDGLGMSARGVTVSTVGLVPRIRQLTEEGVPLTLALSLHAPDDELRNELVPINTRFSVAETVDAAWQYARTTKRRVSIEYAMMRGINDQGWRADLLADVLYATGADWGWVHVNLIPLNPTPGSKWTASDPADEQEFVRRLEARGIPTTVRDTRGREIDGACGQLAASQLPC",
    "response#3": "MTSSELKELAVGMGEKPFRGAQLADWIYRKAVLNYEEMTNLPAAFIARLQDSLALTPLTSRVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGSGEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLHSPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALAKLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKERNHNTLCISTQVGCAVGCKFCFTTKDGLIRNLTTAEIVDQYIQSQRFVGLDNRISNIVFMGMGEPLANYDNVKKAVQIFTDERMAGLSNRKITISSSGILAQIKRMYSDKEFPQVRLAISLNASDQKTREFLMPISETNRLENLMALLNSLPVKTGYRIMLEYVLIKGVNDSPKDAHRLAKLIGGNKKRYKVNLIPFNPYPSSPFERPEKERVEKFQKILWQYNIGAFIRWSKGQDIAAACGQLRRG"
  },
  {
    "reference": "MEPAQKKLRQESKNPYLAHLNNGDDSEEVVSSKGLTRRATTVAQAAKAEEGPNNFFNDKPFSQNYFKILETRRELPVYQQREEFLKIYHENQIIVFVGETGSGKTTQIPQFVLYDELPHLTNTQIACTQPRRVAAMSVAKRVADEMDVDLGEEVGYNIRFEDCSGPNTLLKYMTDGMLLREAMTDHMLSRYSCIILDEAHERTLATDILMGLMKRLATRRPDLKIIVMSATLDAKKFQKYFFDAPLLAVPGRTYPVEIYYTQEPERDYLEAALRTVLQIHVEEGPGDILVFLTGEEEIEDACRKITLEADDLVREGAAGPLKVYPLYGSLPPNQQQRIFEPTPEDTKSGYGRKVVISTNIAETSLTIDGIVYVVDPGFSKQKIYNPRIRVESLLVSPISKASAQQRAGRAGRTRPGKCFRLYTEEAFRKELIEQTYPEILRSNLSSTVLELKKLGIDDLVHFDYMDPPAPETMMRALEELNYLNCLDDNGDLTPLGRKASEFPLDPNLAVMLIRSPEFYCSNEVLSLTALLSVPNVFVRPNSARKLADEMRQQFTHPDGDHLTLLNVYHAYKSGEGTADWCWNHFLSHRALISADNVRKQLRRTMERQEVELISTPFDDKNYYVNIRRALVSGFFMQVAKKSANGKNYVTMKDNQVVSLHPSCGLSVTPEWVVYNEFVLTTKSFIRNVTAIRPEWLIELAPNYYDLDDFDNNKEVKSALQKVYQMAARSKKNARR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP binding.2. For general function, the protein need meet that Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA.3. The protein design should include a DEAH box motif to ensure proper function.4. The presence of Helicase ATP-binding, Helicase C-terminal domains is critical for the interaction of the designed protein with its intended targets.5. We are looking for a protein with broad substrate specificity for ATP + H2O = ADP + H(+) + phosphate.6. A protein that can perform ATP binding, ATP hydrolysis activity, RNA binding, RNA helicase activity in spliceosomal complex, nucleus, post-mRNA release spliceosomal complex, U2-type spliceosomal complex to support rRNA processing, spliceosomal complex disassembly. The designed protein sequence is ",
    "response#1": "LHVLLSLLEKDAPKSGIIFVAEQSEKSKKAGHPPSTTVVVEFLRTTYMGSLEVLLLEEDMNFNARATSFTEVKGKGFLLVSTDIASRGFDLPQTSHIYNFDLPKTAIDYLHRAGRTGESLPIFPLREAFLQAVSEHQLLVVIGETGSGKTTQMAQYLAEAGYGTRGKIGCTQPRRVAAMSVSKRVAEEFGCQLGQEVGYAIRFEDCTSPETIIKFMTDGILLRECLLDPNLSAYSVIILDEAHERTISTDVLFGLLKQALQRRPELKVLITSATLEAEKFSKYFMNAQLFIIPGRTFPVDIFYMGYTVTQLVEFLNTDEKRKRRLLDIISKRQYRPPIVVFLNYKRDCEAVSDALVAAGWRTAIIHGGKQQEQREQAVQHLKRGAVDVLVATDVAGRGLDIPNVSLVVNFQMANNIESYTHRIGRTGRAGKRGTAVTFLGQEDDDVLFELKQMISRSEASPNNQELSRRLGEILSSGDFRPPIIVFVNIKRNCDAIAREIKQWGFSSVTLHGSKTQEQREAALASVRNGSTDVLVATDLAGRGIDVPDVSLVINFNMATSIESYTHRIGRTGRAGRRGKRGSAVLLVPESERVSTREVYDYILSFIQQYLGDQSPEILRSAADLIIELLKDSSLDEQGRKKQIEEVLSTELPQDRFSQLVNPTREVYDYILSFIQQYLGDQSPEILRSAADLIIELLKDSSLDEQGRKKQIEEVLSTELPQDRFSQLVNESLPIFPLREAFLQAVSEHQLLVVIGETGSGKTTQMAQYLAEAGYGTRGKIGCTQPRRVAAMSVSKRVAEEFGCQLGQEVGYAIRFEDCTSPETIIKFMTDGILLRECLLDPNLSAYSVIILDEAHERTISTDVLFGLLKQALQRRPELKVLITSATLEAEKFSKYFMNAQLFIIPGRTFPVDI",
    "response#2": "EETIPLGLEQRDVIGISATGSGKTAAFVLPMLAYISRLPPMREENQTEGPYALVMVPTRELAHQIEEETVKFSRYLGFKAVSITGWESIEKQALKLSQGCEIVIATPGRLLDCLERRYVVLNQCNYLVLDEADRMIDMDFEPQVSEVLDVMPCSNLKPEKEDEELEEKKIYRTTYMFSATMGYKDPSPIQRAAIPIALQARDLIGVAVTGSGKTAAFLLPLLVYISDLPPLDEINKHDGPYALIMAPTRELVQQIETEARKFAGPLGFRVVSIVGGHQIEEQAYNLRDGAEIVVATPGRLLDCIERRLLVLSQCCYVIMDEADRMIDLGFEESVNKILDALPVTNEKPDTDEAENAQIMQRYLGGRDRYRQTMMYTATMPPLVERIAKKYLRRPAIVTIGNAGEAVDTVEQQFVTLTPIQCQAIPAIMSGRDVIGISKTGSGKTVAFLLPLVRQIKAQPPLAPDETGPIGLILTPTRELAVQIQEEALKFCKGSGISSICCVGGSELKQQINELKRGVDIIVATPGRFIDLMTLNSGHLLSPTRISFVVMDEADRLFDLGFGPQVNQIMGCIRPDKQCVLFSATFPSKLKHFASRTLKNPIQITINSKSLINENIQQKIPHPMRNWEETIPLGLEQRDVIGISATGSGKTAAFVLPMLAYISRLPPMREENQTEGPYALVMVPTRELAHQIEEETVKFSRYLGFKAVSITGWESIEKQALKLSQGCEIVIATPGRLLDCLERRYVVLNQCNYLVLDEADRMIDMDFEPQVSEVLDVMPCSNLKPEKEDEELEEKKIYRTTYMFSATMLLSVERLARKFLRNPVVVTIGETTKFITQ",
    "response#3": "GYDEPTPIQRAAIPIAQQARDLIGVAVTGSGKTAAFLLPLLVYISELPPLTEFNKNDGPYALILAPTRELVQQIETEARKFAGPLGFTVVSIVGGHSLEEQAFALRNGAEIIVATPGRLVDCLERRLLVFSQCCYIIMDEADRMIDQGFEEPLTKILDALPVANEKPDTEDAENSQLMSRYLGGKDRYRQTMMYTATMPPLVEKIAKKYLRRPAIVTIGNAGEAVDTVEQRIQSHLYPIAFGTDENILLCAPTGAGKTNVAMLCILNELQKHLREDLSFNLQNFKIVYIAPLKALVQEMVNNFSKRLTPYNIRVAELTGDSQLTKQQISETQIIVTTPEKWDIITRKANDLSYVNLVRLVIIDEVHLLHDERGPVLESIVARIFRHQEETLEQVRLVGLSATLPNYIGYKEPTPIQRAAIPIALGIRDVIGVAETGSGKTASFLIPLISYICELPKLDERSKVNGPYGLILAPTRELAMQIKDEAVKFCAPLGFKVVSVVGGYSAQEQALAVQEGAELIVATPGRLLDVIDRRLLVLNQCCYVVMDEADRMVDMGFEEQVQKVLASLPSSNAKPDSDEAENLAAVSTRRYRQTMMYTATMPVAIEKLAKKYLRRPGIVTIGSAGQAGSTVTQRVEQRVEMISDDSKKWRRVEEILESNRFSPPIIIFVNLKRNIEAIAKQLNAIGWHAVTLHGSKSQEQRERAIEQLRNKTADILVATDIAGRGIDIPNVSLVLNYNMAKSIEDYTHRIGRTGRAGKSGTAITFLGPEDTDVYYDLRVLLSRSAKAHIPDELRNH"
  },
  {
    "reference": "MGVLGGDAHVPIGSQVSPGSVVVTNNESFGHRKLLKGVDFLVRIKAFAFCLAVIVLLKNNVQTTVIAPGIVLQAKYNNTKAPVSLLVLASICCGYAFLQAVVSLLSFIRDKRVLNNTVLAWLTFLLDQVLTYLLLGSAAATAEAAYIAKRGEDKVQWKAVCGPFKRFCDHFAATVFLSFIAVIAFAVSAAISAYYLFRRSKGFK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The Extracellular topological domain of the protein should be designed to be flexible or rigid.2. The designed protein should be expressed specifically in the plasma membrane The designed protein sequence is ",
    "response#1": "SGEMKNCSFNITTQTRGRKREYALFYNLDVVQLESDKTSTSYRLISCNTSVITQACPKISFEPIPIHYCAPAGFAILKCNDKQFNGTGPCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEDIIIRSQNISDNAKSIIVQLNESISINCIRPGNNTRKSIHMGPGKVFYATGDIIGNIRQAHCNISKAKWNNTLRQIARKLGEQFNNKTIVFNQSSGGDPEIVMHTFNCGGEFFYCNTTSLFNSTWENDTNITEESNSSDDTITLQCKIKQIINLWQEVGKAMYAPPISGYINCSSNITGLILVRDGGNNRTSESETFRPEGGNMKDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQRQKR",
    "response#2": "MGAASVALTVQARLLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQIMGIWGCSGKYICTTAVPWNTSWSNKSYDQIWKNMTWMQWEKEIDNYTSEIYSLIALSQDQQEKNEQELLELDKWASLWNWFDISNWLWYIKIFIMIVGGLVGLRIVFAILSIMNRVRQGYSPLSFQF",
    "response#3": "MGAASVALTVQARLLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQIMGIWGCSGKYICTTAVPWNTSWSNKSYDQIWKNMTWMQWEKEIDNYTSEIYSLIALSQDQQEKNEQELLELDKWASLWNWFDISNWLWYIKIFIMIVGGLVGLRIVFAILSIMNRVRQGYSPLSFQL"
  },
  {
    "reference": "MVDSHLTFTGFRDRRIHLGVTGSIAAFKALDLLRDWKRAGIQVGASLTESAQEFVTPLSMQALGADPVDTKLFPAGDNVYAHLAPGQGAHCYAIAPATANILAKLAYGLADDVVSCQVLAHPGPIIVAPAMNPNMWLNPAVQENWQKLQERFMICLAPDSGEVACGDMGTGRLARHELIFLHGLKALSPQDLSGTRVLVTLGPTREYFDPVRFWSNPSSGTMGASIAVAAWLRGADVTAICGPVSLWLPPGIHRISVTSAVEMHEAATDIWPSADVAVCTAAVADFAPKPYGETKFKKTGEPLQVEFSANPDIIAALSNTRHVRQRIIGFAAETGDLAQLAREKRERKGMDIIVANHVLTPGAGFAAPTNEVTVIDANGREESWPTLPKTEVAWRLLDWLSLL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must have a Proton donor active site that is highly conserved among related enzymes.2. The designed protein must possess metal ion binding to support pantothenate catabolic process, coenzyme A biosynthetic process under certain conditions. The designed protein sequence is ",
    "response#1": "EERSVVITGGGTREKIDEVRYISNFSSGKMAKSIALSLYFKGASVCYITTKGNAGLPKNIRTIDVEDAKEMLKSTKDAILSAKENTQTVKRPFLFMVAAVADFTPKHPKQGKLKKSALGENWQIELEQTSDILSTLLKDREGVTTIAFKAEMDDQEGLNNAKNLLAQKKVDAVCYNLLQDAKSFGGEENSITFG",
    "response#2": "EERSVVITGGGTREKIDEVRYISNFSSGKMAKSIALSLYFKGASVCYITTKGNAGLPKNIRTIDVEDAKEMLKSTKDAILSAKENTQTVKRPFLFMVAAVADFTPKHPKQGKLKKSALGENWQIELEQTSDILSTLLKDREGVTTIAFKAEMDDQEGLNNAKNLLAQKKVDAVCYNLLQDAKSFGGEENSITFGFL",
    "response#3": "EERSVVITGGGTREKIDEVRYISNFSSGKMAKSIALSLYFKGASVCYITTKGNAGLPKNIRTIDVEDAKEMLKSTKDAILSAKENTQTVKRPFLFMVAAVADFTPKHPKQGKLKKSALGENWQIELEQTSDILSTLLKDREGVTTIAFKAEMDDQEGLNNAKNLLAQKKVDAVCYNLLQDAKSFGGEENSITFLG"
  },
  {
    "reference": "MVGYWEKKSGGGSSSNGRVFFGGFVVMYDADTEETLVIGKDIINYIFSNNLDGEDTPLNINHREDALVGRVFKFFDVDRGIFCVGEITSKRFLRMIRDASRDSSVVELGPTADCVPRDSVLEYLSVYLPALSLSNFVTPSDSRPFFRHVSLCGLGRRRGTLTVYGRSVPWIVSQFKCLSVDDAKKILSFPVPAPVSPYAQETFCLSPEFLLATSTDITCIDRRSNTLSYDKKLAGVSNESYVRASVTCRPSPSARNNNSLCGSRHGGVQQYGTDDSIRGGVVPYDSQRRRFNHRPEENVSTVSIKMNKFLENGEGVYLSKDVFLGLLRQTGGYRGLPSCGGLYEPIPHICYQPHGLWSAGGAGGSNMLLGGDDDQFNPAPKRRRLEYPALPNERGGGGGASPEKPIVQALSTPPPPQPPTPVTTFRPHNDDLTVSAIKQMCSTLADIRSDLDLLKKKSEIRDQMLQQQHLAPQQTTTSYTHAQQPQQPHAVPAPHHVQQQQQTPPAQHPQQAPSSHPGVLDNPPPQGPGSQAPTQAPPQHLVDPVALLRDSQNADALIQQPGVPPVEIVNAGYVFNKNGETADNKTQMLSRNRDMFISALGCD",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The Charge relay system active site of the designed protein must be optimized for substrate binding and specificity.2. Target a Polar residues compositional bias in the protein's composition for improved properties.3. The protein must exhibit the following characteristics: Cleavages products are evicted from the capsid before or during DNA packaging.; Capsid scaffolding protein: Acts as a scaffold protein by binding major capsid protein in the cytoplasm, inducing the nuclear localization of both proteins.4. The protein should be optimized for stability and solubility when expressed in host cell cytoplasm where it will be involved in viral release from host cell. The designed protein sequence is ",
    "response#1": "MIS",
    "response#2": "SHHHHHH",
    "response#3": "MSSKL"
  },
  {
    "reference": "MLNGKKILLGVTGGIAAYKAATLCSMLTKAGAQVQVIMTESAQQFIPALTLQVLTHHPVHTDTFDERNPKVVNHIDLADWADLVLIAPATANVIGKLAHGLADDMLSTTLLAVTAPVMIAPAMNVHMYTHPAVAQNMQTLVDRGVMMIEPGEGMLACGYVGKGRLEEPETIVDVLRRFFEQREAGGNSSFAPLSSRPSAANAGEPEPSFVDPGPLADIAVAEEGGEQLFATAGGAAERGLAGSLTSQDSVATDKIEGPLSGKKVLVTAGGTIERIDPVRYITNDSSGKMGVALAEAARDLGADVKLIAGKVQIPLPAGIPTLRIESAEDMYEACLNAWPDTDMLIMSAAIADYRPKSSSPRKIKKIGDTMALELVKTVDVLQTLGAQKEHQFLIGFAAETHDLDAYAAEKLQRKNCDMLVANDVTQTGAGFGSDTNIVRLFGPEGPIADLPKMSKYDTAREILLEASRRLGQGALR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must possess phosphopantothenoylcysteine decarboxylase activity, metal ion binding, phosphopantothenate--cysteine ligase activity to support pantothenate catabolic process under certain conditions.2. The protein should have a high affinity for CTP.3. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.4. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.5. The protein should be optimized for activity in coenzyme A biosynthesis, Cofactor biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.6. A key design requirement for the protein is the optimization of the Proton donor to achieve high catalytic efficiency for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.7. The designed protein must have at least one Flavoprotein domain. The designed protein sequence is ",
    "response#1": "KHILLAVSGGIAAYKSAELVRLLRKQRAEVRVVMTHAAIQFVAPLTFQSLSGNPVHSELLDSAQENAMGHINLARWADVLLVAPATANCIAKFSQGLADDLLSTLYLAATCPVYIAPAMNQAMWNKAVTQENITKLISHGVKVIGPGKGDQACGENGLGRMAEPSEICVPVLITAGPTREPIDPVRYLSNYSTGSMGFAIAAAAQERGAEVTLVTGPTEMQPPDVTRLVRVQTASEMLEAVRNHFGSCRVLISAAAVSDFSPVKSPRKLKKLEQEGERRTLELHQTPDILAEMGRQKQNQILVGFALETEDLITNARQKLEGKNLDMIVANSAGEGEQPFGPGAVVAGGVEAFEPMPKATLARKLLDRVLAHVEPSGD",
    "response#2": "KRILLGISGGIAAYKSADLVRRLKERGAEVRVILTNAAQQFITPLTLQAVSGNPVSTSMLDPAAEAAMGHIELAKWANLIVVAPASADVIAKLAHGMANDLLTTCVLATAAPVAVAPAMNQQMYNNIATQQNLATLKKHKFHIYGPGAGEQACGDVGYGRMLEPLELVALIEAALGDGPLRGVRILVTAGPTEEPVDAVRHISNPSSGKMGYAIAAEAVRMGAEVTLVTGPTLLDPPAGAKTIRVRTAQQMLDACLQEYEQVDVVIATAAVSDYRPAEVWQGKRKKGEEEWTIRLVPNPDILRTLGERKGKHVLVGFAAETQELLANAQAKLREKNLDLIVANDVSQEGSGFRTDTNRVTLIDAGGVEDWPTLSKDEVAARLAARIAEALA",
    "response#3": "RIVLGITGGIAAYKAADLVRRLKEAGADVQVVMTAAAQRFVGAQTFQALSGRPVRDSLWDAAAEAAMGHIELARWPDLVLIAPASADTIARLAQGRADDLLTTLCLATDRPLAIAPAMNRLMWAHPATQQNLQVLQARGVQVLGPGSGSQACGETGDGRMWGRIEDESLRAIREEKDCTPQQRACNGGVYVFRVEALREALRRIGNDNAQQEFYLTDAVRVLRSDGAVVAAVATDDRDHLLGINDRVQLAEAGRILNERIVRRWMLSA"
  },
  {
    "reference": "MDSQNFIITTAQMMAIESQVFAAGMPVAALMEKAAMALTSTILALEIAPAVKTVGVLVGPGHNGGDALVVARELALRGREVWLYVPLGEVKPLTQNHLDYADYLGLPRVESVAGLADCDLIIDGLFGFGLTRPITGELAGAIATLNRMTQTVISIDLPSGIHTDTGAVLGAAIRADYTFCLGLWKRAFFQEQALPFLGQVACLDLGLPPQAIAAVLDEVPIHQRFTQPQFEAVLPLPRAVVSHKYQQGHTLLICGSQQYAGSAILTGLGARASGVGMLTIAVPAALKPLVVAQLPEALVVGCPETPEGAIAALPLDLSRYDAIACGPGLTLGAGEILPQVLAAPVPLVLDADGLNLLGTEVERLRERPAPTILTPHWGEFQRLFPDLEPGDRFVAALAAAAQAGAIVLLKGARTAIASPDGDFWVIPDSTPGLARGGSGDVLTGLLAGLWASAKAPAVEVAAAAAFWHAAAGKRAAAAHTELGVDPLRLAATLGACLA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. The protein have enhanced (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.3. The protein must exhibit NADPHX epimerase activity, NADHX epimerase activity.4. The designed protein have YjeF N-terminal domains with specific sequence motifs that are crucial for its activity.5. The (6S)-NADPHX binding site should be stable and able to withstand changes in environmental conditions.6. The protein must exhibit the following characteristics: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.. The designed protein sequence is ",
    "response#1": "MRSADQFTIANKPIASIDLMEKAARAFVQCFLREEFDTSKSVAIFCGKGNNGGDGLAIAHLLLANGYENLKVYIVDFSEKQSGDFAINLQRMEETRCKKTIISQAADLKNLKADIIIDAILGSGLNKPLSGEFEALARTINKLNRKVYAVDVPTGFFAEGKLPKDYNGVKAYKTICFQRPKINFFFPESAMATEKYEVADIDLDEAFIESHKGENGILVGIAGSVDYYGAPIYMSKGALRMGIDLLFLFSPEYIHNYISSSTHDIILKKSGERYIEFNNELKRIIEDKATAVALGCGIGKNEVSQESSTKIIEESLLVKKPIVIDADALDSVTLFDDLKGFAVITPHRGEFKRIFSIDPDENNVVKMAKKYNLVILLKGSVDIISDGEIVKKNKSFHHPSMTRGGTGDILTGAVGGLLSRGIDTFHSATLASFIVGSAGKMAFEEKSNSYFTSEVIDMIS",
    "response#2": "MRSADQFTIANKPIASIDLMEKAARAFVQCFLREEFDTSKSVAIFCGKGNNGGDGLAIAHLLLANGYENLKVYIVDFSEKQSGDFAINLQRMEETRCKKTIISQAADLKNLKADIIIDAILGSGLNKPLSGEFEALARTINKLNRKVYAVDVPTGFFAEGKLPKDYNGVKAYKTICFQRPKINFFFPESAMATEKYEVADIDLDEAFINPDESHKGENGILVGIAGSVDYYGAPIYMSKGALRMGIDLLFLFSPEYIHNYISSSTHDIILKKSGERYIEFNNELKRIIEDKATAVALGCGIGKNEVSQESSTKIIEESLLVKKPIVIDADALDSVTLFDDLKGFAVITPHRGEFKRIFSIDPDENNVVKMAKKYNLVILLKGSVDIISDGEIVKKNKSFHHPSMTRGGTGDILTGAVGGLLSRGIDTFHSATLASFIVGSAGKMAFEEKSNSYFTSEVIDMIPQVFNPDESHKGENGILVGIAGSVDYYGAPIYMSKGALRMGIDLLFLFSPEYIHNYISSSTHDIILKKSGERYIEFNNELKRIIEDKATAVALGCGIGKNEVSQESSTKIIEESLLVKKPIVIDADALDSVTLFDDLKGFAVITPHRGEFKRIFSIDPDENNVVKMAKKYNLVILLKGSVDIISDGEIVKKNKSFHHPSMTRGGTGDILTGAVGGLLSRGIDTFHSATLASFIVGSAGKMAFEEKSNSYFTSEVIDMIPQVFSS",
    "response#3": "MRSADQFTIANKPIASIDLMEKAARAFVQCFLREEFDTSKSVAIFCGKGNNGGDGLAIAHLLLANGYENLKVYIVDFSEKQSGDFAINLQRMEETRCKKTIISQAADLKNLKADIIIDAILGSGLNKPLSGEFEALARTINKLNRKVYAVDVPTGFFAEGKLPKDYNGVKAYKTICFQRPKINFFFPESAMATEKYEVADIDLDEAFINPDESHKGENGILVGIAGSVDYYGAPIYMSKGALRMGIDLLFLFSPEYIHNYISSSTHDIILKKSGERYIEFNNELKRIIEDKATAVALGCGIGKNEVSQESSTKIIEESLLVKKPIVIDADALDSVTLFDDLKGFAVITPHRGEFKRIFSIDPDENNVVKMAKKYNLVILLKGSVDIISDGEIVKKNKSFHHPSMTRGGTGDILTGAVGGLLSRGIDTFHSATLASFIVGSAGKMAFEEKSNSYFTSEVIDMIPQVFESHKGENGILVGIAGSVDYYGAPIYMSKGALRMGIDLLFLFSPEYIHNYISSSTHDIILKKSGERYIEFNNELKRIIEDKATAVALGCGIGKNEVSQESSTKIIEESLLVKKPIVIDADALDSVTLFDDLKGFAVITPHRGEFKRIFSIDPDENNVVKMAKKYNLVILLKGSVDIISDGEIVKKNKSFHHPSMTRGGTGDILTGAVGGLLSRGIDTFHSATLASFIVGSAGKMAFEEKSNSYFTSEVIDMIA"
  },
  {
    "reference": "MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLAGMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQAKPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCWALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that can perform amino acid transmembrane transporter activity, efflux transmembrane transporter activity in plasma membrane to support amino acid transport, cysteine export across plasma membrane.2. The protein must exhibit the following characteristics: Exporter of O-acetylserine (OAS) and cysteine.. The designed protein sequence is ",
    "response#1": "YFVFNSRTAYAIPILAFAFVCHPEVLPIYSELKDRSRRKMQTVSNISITGMLVMYLLAALFGYLTFYGEVEDELLHAYSKVYTFDIPLLMVRLAVLVAVTLTVPIVLFPIRTSVTTLLFPKRPFSWIRHFLIAAVLIALNNVLVILVPTIKYIFGFIGASSATMLIFILPAVFYLKLVKKESFRSPQKVGALIFLVVGIIFMIGSMALIY",
    "response#2": "RTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWVAGTTVLVAFAIITYYTSTLLADCYRSPDSITGTRNYNYMGVVRSYLGGKKVQLCGVAQYVNLVGVTIGYTITASISLVAIGKSNCYHDKGHKAKCSVSNYPYMAAFGIVQIILSQLPNFHKLSFLSIIAAVMSFSYASIGIGLAIATVASGKIGKTELTGTVIGVDVTASEKVWKLFQAIGDIAFSYAFTTILIEIQDTLRSSPPENKVMKRASLVGVSTTTVFYILCGCIGYAAFGNQAPGDFLTDFGFYEPYWLIDFANACIALHLIGAYQVYAQPFFQFVEENCNKKWPQSNFINKEYSSKVPLLGKCRVNLFRLVWRTCYVVLTTFVAMIFPFFNAILGLLGAFAFWPLTVYFPVAMHIAQAKVKKYSRRWLALNLLVLVCLIVSALAAVGSIIGLF",
    "response#3": "YFVFNSRTAYAIPILAFAFVCHPEVLPIYSELKDRSRRKMQTVSNISITGMLVMYLLAALFGYLTFYGEVEDELLHAYSKVYTFDIPLLMVRLAVLVAVTLTVPIVLFPIRTSVTTLLFPKRPFSWIRHFLIAAVLIALNNVLVILVPTIKYIFGFIGASSATMLIFILPAVFYLKLVKKESFRSPQKVGALIFLVVGIIFMIGSMALIF"
  },
  {
    "reference": "MHLEHLADGMDLPDTADGLSIEGLTADSRKVQPGFLFAAFKGSTVDGVQFVPKAVEARAVAVLCATNAAEAVRALVPPTVAVLSADEPRLTFSKMAARFYGRQPDTIVAVTGTAGKTSVAHFVRQIFQAAGHPAASLGTLGIIKPDGESYGGLTTPDPVQLQKDLADLATEGIRYAALEASSHGLDQYRLDGVRLTAAAFTNLGRDHMDYHPTIEDYLQAKLRLFSELLPAGGHVVVDPGEKYTDRVLAIAEERGLPVFTVGETGKDLKLTGLRPSGAGQLLTVETSRGEYKVTLPLIGRFQVSNALIAAGLAITAGIDAGAALRALENLKGAPGRLELAGTTPAGGMVFVDYAHKPDALDKALAALKPFAEGSLSVVVGAGGDRDPGKRPLMGEAAARHADLVIVTDDNPRSEDPATIRKAVLAGAPGGLEIGDRAEAIAEGIRRLKSGDILCVAGKGHETGQIVGDTVIPFSDHDSVRQALNQLGLK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must have high specificity towards magnesium ion binding.2. A protein that can modulate the overall flux of metabolites through the Cell wall biogenesis pathway, enhancing or suppressing its activity as needed.3. The designed protein must have at least one Mur ligase C-terminal domain.4. The protein should be able to bind UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate ligand in a variety of conditions The designed protein sequence is ",
    "response#1": "VTGTNGKTTSTFMVKSILEAAGYKVGLIGTISNYIGDKKIPSHRTTPESLELQKLFGQMVKESVDYCVMETSSHSLYLDRTYGVKFCEAIFTNLTRDHLDFHKTFENYYRAKLILFQNAFNSIINVDDSYGERVYRDAPGHKVTYAIDRQADVRGSNLNVHSRGINFNVSYEGSTESVNLNIPGKYNAMNALGSAAALELARRLGVPVESIVSGLEEFKGVKGRFDYIVSPVDRIMGIVDYAHTPDALLNVLSTLQKLRKGGEKIITVVGAGGDRDKTKRPVMAEVACEHSDRVILTADNPRSEDPMDILRDMESGLGSAAKRKYLVVPDRTEAIKTAITLAGKGDIILVAGKGHETYQIVGDRVLPFDDREVARELLRERGWL",
    "response#2": "VTGTNGKTSVTSFIYQLYRLLKQGCTLIGTNGIKINDKCLPSDNTTPNTLIINQILADSLKQGIINAVMEVSSHAIKQKRVSQLDFHTVILTNFSQDHLDYHRTMLDYFYTKGMFLASLGNSYNDKKVLFNGDDKTYKKFLDLTNVQSYTYGITTLNDIQAKNIICGIDQISFHLYCFKEFCGIVESKLFGFFNIYNLLAALNT",
    "response#3": "ITGTNGKTTVATLLHRLARMFDRKAGLVSTVENRINDRVVPATHTTPDPVGLQALFADMLDAGCTHCFMEASSHALDQHRLSGSQLTGGVFTNITHDHLDYHGDFNAYISAKKSLFDKLPGVAFALTNADARHGDTMVQNTRARVVTYGTHQVADHKARIIENGLGGLQLHLDGQDLYTRLIGEFNASNLTAVYAVV"
  },
  {
    "reference": "MGLHSNQEYFVSWEELHRATRELARRQLPAEQWQGIIAVSRGGLVPAAILARELNIRVVESVCVRSYAHQSQVAEPEMLKDISCTEDGAGFLVVDDLVDTGNTLRFLRKRLPKAKFITVYAKPEGLPLVDEYEADIEQSTWIHFPWDVHLHYVDPLAGEDK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. A protein should be able to manipulate the activity of enzymes in the XMP biosynthesis via salvage pathway pathway, either by activating or inhibiting their function.2. The designed protein should be able to compete with endogenous proteins or inhibitors for xanthine binding.3. A protein with guanine phosphoribosyltransferase activity functions is required to enable GMP salvage, purine ribonucleoside salvage. The designed protein sequence is ",
    "response#1": "LNEVGPFAAVIAITRGGLVPAAIVARELGVRVIDTVCIASYEHDRQGELQVLKGVSDSTKALGGGTGKGLLIVDDLVDTGATARLVREMLPDAHFATVYAKPKGR",
    "response#2": "WEELHRTSKALAWRLLELGPFKGIIAVTRGGLVPAAIVARELEIRLIETACLASYHAQERGEVEVMKAPEMAGDGEGWLIIDDLVDTGETGKMLRQLLPKAHFATVYAKPQGRPVVDTFITEVSQDTWIFFPWDLV",
    "response#3": "WEELHRATKELARRQLPAEQWRGIIAVSRGGLVPAAIVSRELNIRLVDSVSVSSYNHTEQRGDLQLLKDVTASEDGEGFLVIDDLVDSGNTLAFLRQRLPRAKFITVYAKPKGLATVDDYVADLDQETWIHFPWDMHLE"
  },
  {
    "reference": "MFSITSTTRIKVSLPKEKENSDSPADTHISHSYPHPPHQHSSTLIHPPPPQSVTHLVSTVSXXXXXXXXXLCKFCDVQATTCTGKGSCKVECNITSICTHDDDVCVSIWRKKDDNVTFDTVCHNPAQKLYGLVLDDYNNSKCEMKERKGMGTEFFICSCSEDECNEKIFFNSNKESPVVEVILVSLVPLLVIAVLVITSFYCYRVYRQRQANSKRKKNPALDSDALAIMNDEEGSDSSSTLANNLNHNTELLPIELDVQVGKGRFAEVYKAKLKQGSSVSEEQGFETVAIKIFPYEEYASWKNEKDIFSDADLRHENVLHFLTAEERKVQRQYWLITAYQPRGNLQDYLTHHVVSWPDMWLLGSSLARGVAHLHSDHTPCGCFKLAIAHRDIKSSNILVKSDLTCCLCDFGLALRLDSNLSVDELANSGQVGTARYMAPEVLESRINLENIESFKQADVYSMALVLWEITSRCSAIGEVKEYEPPFGNLREHPCVESMKDSVLRDRGRPEIPNTWTSHTGIQIVCASIEDCWDHDPEARLTAQCVAERFDDIEFLDKLSERSDSEEKIPEEIFVVDEK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must be engineered to have a high affinity for Mn(2+) in order to increase its catalytic activity.2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.3. For general function, the protein need meet that Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis; The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2; This constitutes the canonical SMAD-dependent TGF-beta signaling cascade4. The protein should have Helical domains to ensure proper insertion into the membrane.5. A protein that contributes to protein phosphorylation by functioning in cytoplasm, plasma membrane, receptor complex with ATP binding, transforming growth factor beta receptor activity, type II, metal ion binding.6. The protein contains novel Protein kinase domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "LEDVVGRGRFAEVWRARLLQSDMGGVNSYETVAVKVFPNVEYASWRNECSILSDPTLQHENLVQFLAAEERSLPSDTLRTYWIVLAHYSLGNMQDHLTANTLSWEELVSLASSIANGLAHLHSETKLNGAPKVPVAHRDLKSSNILIKNRKQCVLCDFGLALRLDISLTVDDYANSGQVGTARYMAPEVLESRVNLEDLEAFKQMDIYSMALVLWEMASRCDVIEEVKAYQPAFGSKVCEQPCVDSMRDLVLRDRGRPDIPSAWTQHQGMSVFCSTITECWDHDPEARLTAHCVLEDVVGRGRFAEVWRARLLQSDMGGVNSYETVAVKVFPNVEYASWRNECSILSDPTLQHENLVQFLAAEERSLPSDTLRTYWIVLAHYSLGNMQDHLTANTLSWEELVSLASSIANGLAHLHSETKLNGAPKVPVAHRDLKSSNILIKNRKQCVLCDFGLALRLDISLTVDDYANSGQVGTARYMAPEVLESRVNLEDLEAFKQMDIYSMALVLWEMASRCDVIEEVKAYQPAFGSKVCEQPCVDSMRDLVLRDRGRPDIPSAWTQHQGMSVFCSTITECWDHDPEARLTAHCVELDVQVGKGRFAEVYRAKLKQGSSVSMEQGFETIAVKIFSHEEYPSWKNEKEIFSDADLRHENVLHFLTAEDRKAQRQYWLITAYYPRGNLQEYLTHNIVSWHDLWLLGSSLALGVAHLHSDRTPCGRSKVPIAHRDIKSSNILVKNDLTCCLCDFGLALRLDSSLSVDELANSGQVGTARYMAPEVLESRINLENIESFKQADVYSMALVLWEIISRCSAVGEVKAYEPPFGNLREHPCVESMKDSVLRDRGRPEIPNNWINHIGIQMVCASIEECWDHDPEARLTAHCVA",
    "response#2": "EVLVGKGRFAEVWRACLLGTERGGVSSCQTMAVKVFPAVEYASWRNECAIFSDPELQHDNVVQFLAAEEKGPPSHALRSYWLVLAYHSLGNLQEFLTANILSWEELVAMAGSIAKGLAHLHSDTTPRGASKVPVAHRDLKSSNIVVKSRNECALCDFGLALRLDISLTVDDYANSGQVGTARYMAPEVLESRVNLEDLEAFKQMDVYSMALVLWEMASRCHAIGEVKSYEPAFGSKVCEQPCVDSMRDLVLRDRGRPDIPSTWTQHQGMNVFCSTITECWDHDPEARLTAHCVIKLEMLLGKGRFAEVWRARLLQGQKGGVDSNKTVAVKVFPVMEYTSWRNECSIFSETTLQHDNVIQFLASEERGPPGKTYWLVLAYYSLGNLQDYLIANTLSWEELVHMAGSIAKGLAHLHCDTTCDGEPKVPVAHRDLKCSNILVKNKKECALCDFGLALRLDLSLTVDDYAHSGQVGTARYMAPEVLESRVNLEDLEAFKQMDVYSMALVLWEMASRCEAIGEVKSYEPVFGSKVCEQPCVDSMRDLVLRDRGRPDIPPTWTHHQGMSVFCSTITECWDHDPEARLTAHCVVERFTAIELDTLVGKGRFAEVYKAKLKQNTSEQFETVAVKIFSYEEYASWKTEKDIFSDINLKHENILQFLTAEERKTDLGKQYWLITAFHSRGNLQEYLIKHIISWEDLWKLGGSLARGIAHLHSDHTPCGRPKTPIVHRDLKSSNILVKNDLACCLCDFGLSLRLDPTLSVDDLANSGQVGTARYMAPEVLESRMNLDNVESFKQTDVYSMALVLWEMTSRCNGIGEEVKDYEPPFGSKVREHPCVESMKDNVLRDRGRPEIPCSWLNHQGIQKVCETIVECWDHDPEARLTAQCVA",
    "response#3": "LQVGKGRFAEVWKARLLQGERGGVNIYETVAVKVFPAMEYASWRNECSIFSDPQLEHDNVVKFLAAEQRESPGHSLQTYWLVLAYHSLGNLQDFLTVNIVSWEELIAIASSIAKGLAHLHSDTTPNGVPKVPVAHRDLKSSNIVMKSRRECALCDFGLALRLDLSLTVDDYANSGQVGTARYMAPEVLESRVNLEDLEAFKQMDVYSMALVLWELASRCHAIGEVKNYEPAFGSKVCEQPCVDSMRDLVLRDRGRPEIPSAWTQKPGMNVFCSTITECWDHDPEARLTAHCVIELDQLVGKGRFAQVFKAKLKQNASDQFETVAVKIFPYEEYASWKNEKDIFSNSDLRHENILHFLTAEEKKAQRQYWLITAFHARGNLQEYLTHNVISWEELKILGSSLAQGVSHLHSDRLPCGRPKVAIVHRDLKSSNILVKSDLTCCLCDFGLGLQLDSSLSVDDLANSGQVGTARYMAPEVLEARLNLENTESFKQADIYSMALVLWETSRCDAVGEVKDYEPAYGSKVREHCVESMKDNVLRDRRRPEIPDTWLRRQGVAMMCGTIMECWDHDPEARLTAHCVAKRIS"
  },
  {
    "reference": "MSEPLKPRIDFAEPLKEEPTSAFKAQQTFSEAESRTFAPAAIDERPEDEGVAEAAVDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVALGGCAAGALIVGAGVGSVVTEWRRLWRLRQRAHERDEARELLHSHSVGKGRAFCEKLAQQAGIDQSHPALQRWYAAIHETQNDREIVGLYAHLVQPVLDAQARREISRFAAESTLMIAVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFRLVLLNIAFAGASELVREVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDNDKPRLGDFRRQLIGQLKETLQKSKSSPEK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have a specific function within the plasma membrane.2. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "STTPPWWAIPPAWSLGAEIQAYFLLPILLTYKMLGLSVFWISYIIYSLANLNIIHSDYFGYRLIPGVIFMFLSGAYLQKIVSGKASRLEMLSLIIIYIISLFWLVFFIIIKGKYGAYTRETLLGLLVGIPLVYTLLKIRRKFYFNDLFGKLSYGIFLSHFLSFWILEFVNLTQNIISMIFLSLIISASVSYLL",
    "response#2": "STTPPWWAIPPAWSLGAEIQAYFLLPILLTYKMLGLSVFWISYIIYSLANLNIIHSDYFGYRLIPGVIFMFLSGAYLQKIVSGKASRLEMLSLIIIYIISLFWLVFFIIIKGKYGAYTRETLLGLLVGIPLVYTLLKIRRKFYFNDLFGKLSYGIFLSHFLSFWILEFVNLTQNIISMIFLSLIISASVSYLL",
    "response#3": "STTPPWWAIPPAWSLGAEIQAYFLLPILLTYKMLGLSVFWISYIIYSLANLNIIHSDYFGYRLIPGVIFMFLSGAYLQKIVSGKASRLEMLSLIIIYIISLFWLVFFIIIKGKYGAYTRETLLGLLVGIPLVYTLLKIRRKFYFNDLFGKLSYGIFLSHFLSFWILEFVNLTQNIISMIFLSLIISASVSYLL"
  },
  {
    "reference": "MRRSRSSAAAKLRGQKRSGASGASAAPAASAAAALAPSATRTRRSASQAGSKSQAVEKPPSEKPRLRRSSPRAQEEGPGEPPPPELALLPPPPPPPPTPATPTSSASNLDLGEQRERWETFQKRQKLTSEGAAKLLLDTFEYQGLVKHTGGCHCGAVRFEVWASADLHIFDCNCSICKKKQNRHFIVPASRFKLLKGAEHITTYTFNTHKAQHTFCKRCGVQSFYTPRSNPGGFGIAPHCLDEGTVRSMVTEEFNGSDWEKAMKEHKTIKNMSKE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must bind to Zn(2+) in order to perform its enzymatic function.2. The designed protein's functionality will benefit from Pro residues compositional bias in its structure.3. For general function, the protein need meet that Required for distribution of pericentromeric heterochromatin in interphase nuclei and for centromere formation and organization, chromosome alignment and cytokinesis. The designed protein sequence is ",
    "response#1": "VPWMRLMASMFPSHVRMINVGISYEGREIPALRLGAGSNRAQSAPRRTIVMVGGSHAREWISTSTVTYVASNLISNFGKSRAVTRLLEDFDVVLVPTINPDGYVYTWEVDRLWRKSRQRTSLRFCPGIDLDRSWNFEWDGERTRSNPCSENYAGDEPFEGVEAAQFAQWALNETQNNNVDIVGFLDLHSYSQQVLYPFSFSCSSVPPTLETLEELAMGFAKVIRQTTHEIYDVTSACEGTVTATDKASAKTFFPVSGGGSALDWFYHQLHASFAYQIKLRDRGSYGFLIPSEYIVPTGKEL",
    "response#2": "VPWMRLMASMFPSHVRMINVGISYEGREIPALRLGAGSNRAQSAPRRTIVMVGGSHAREWISTSTVTYVASNLISNFGKSRAVTRLLEDFDVVLVPTINPDGYVYTWEVDRLWRKSRQRTSLRFCPGIDLDRSWNFEWDGERTRSNPCSENYAGDEPFEGVEAAQFAQWALNETQNNNVDIVGFLDLHSYSQQVLYPFSFSCSSVPPTLETLEELAMGFAKVIRQTTHEIYDVTSACEGTVTATDKASAKTFFPVSGGGSALDWFYHQLHASFAYQIKLRDRGSYGFLIPSEYIVPTGKEL",
    "response#3": "VPWMRLMASMFPSHVRMINVGISYEGREIPALRLGAGSNRAQSAPRRTIVMVGGSHAREWISTSTVTYVASNLISNFGKSRAVTRLLEDFDVVLVPTINPDGYVYTWEVDRLWRKSRQRTSLRFCPGIDLDRSWNFEWDGERTRSNPCSENYAGDEPFEGVEAAQFAQWALNETQNNNVDIVGFLDLHSYSQQVLYPFSFSCSSVPPTLETLEELAMGFAKVIRQTTHEIYDVTSACEGTVTATDKASAKTFFPVSGGGSALDWFYHQLHASFAYQIKLRDRGSYGFLIPSEYIVPTGKELF"
  },
  {
    "reference": "MMLSLGSDATVLPFHAKNLKFTPKLSTLNGDLAFSKGLGVGRLNCGSVRLNHKQHVRAVGKSFGADENGDGSEDDVVNATIEKSKKVLALQRELIQQIAERKKLVSSIDSDSIPGLEGNGVSYESSEKSLSRDSNPQKGSSSSGSAVETKRWHCFQQLCRSKETETWAVSSVGINQGFDEIEKKNDAVKASSKLHFNEQIKNKLYERPDTKDISSSIRTSSLKFENFEGANEPSSKEVANEAENFESGGEKPPPLAGTNVMNIILVSAECAPWSKTGGLGDVAGSLPKALARRGHRVMIVAPHYGNYAEAHDIGVRKRYKVAGQDMEVTYFHTYIDGVDIVFIDSPIFRNLESNIYGGNRLDILRRMVLFCKAAVEVPWHVPCGGICYGDGNLVFIANDWHTALLPVYLKAYYRDHGLMNYTRSVLVIHNIAHQGRGPVEDFNTVDLSGNYLDLFKMYDPVGGEHFNIFAAGLKTADRIVTVSHGYAWELKTSEGGWGLHNIINESDWKFRGIVNGVDTKDWNPQFDAYLTSDGYTNYNLKTLQTGKRQCKAALQRELGLPVREDVPIISFIGRLDHQKGVDLIAEAIPWMMSHDVQLVMLGTGRADLEQMLKEFEAQHCDKIRSWVGFSVKMAHRITAGSDILLMPSRFEPCGLNQLYAMSYGTVPVVHGVGGLRDTVQPFNPFDESGVGWTFDRAEANKLMAALWNCLLTYKDYKKSWEGIQERGMSQDLSWDNAAQQYEEVLVAAKYQW",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein can be targeted to chloroplast and perform glycogen (starch) synthase activity. The designed protein sequence is ",
    "response#1": "GNPVTVLYNPANTVLNGKPEVWFRGSFNRWTHRLGPLPPQKMEATDDESSHVKTTAKVPLDAYMMDFVFSEKEDGGIFDNKNGLDYHLPVL",
    "response#2": "DEDVEIFLNRGLSTLKNESDVLIMGAFNEWRYRSFTTRLTETHLNGDWWSCKIHVPKEAYRADFVFFNGQDVYDNNDGNDFSITVI",
    "response#3": "DEDIEVFLNRNLSTLNNEPDVLIMGAFNEWRWKSFTRRLEKTWIHEDWLSCLLHIPKEAYKMDFVFFNGQSVYDNNDSKDFCVEIL"
  },
  {
    "reference": "MLFNCFGILALLQILPALAYPPCREQMSDPYEFGESDLMRPGMHDTPLSLMQKREALAISLSKRDSVGSYAPYNVTCPSDYMLRPASDGISSGEQSFIDKRIPKINTQMRSFISNTGLDVDVNSVINDSDGPRLGLAFSGGGLRAMVHGGGVLNAFDSRNGNGSSLAGILQSAMYIAGLSGGSWLVGSVAVNNFANITYLRDNVWNLEHSVFAPHGDNVVENLAYYDDLDDEIDQKKDAGFDTSLTDLWGRALSRKLVDATQGGPNITFSSIRNQTWFQNADYPYPIIISDSRLEEEKAIPANTSIFEFTPYEFGTWDNGIKAFLPMEYVGTHLKNGVPPDHKCIRNYDNAGFVMGTSATLFNTFLLEWSQEVTSNSTLYDIIHKVFEKLSEDQNDIAPYPNPYQNFTTTNTTVKNPFERFDTIDLVDGGEDDENIPIWPLLHPQRFVDVIFAVDATYDDSNGWPDGSSIVTTYERIITYNANKSVDVRGFPYIPDEDTIISLGLNTHPTFFGCDGRNTTAGNHTVDNNTPPLLVYFPNYPWVYYSNISTFTMSMNDTLSSGILENAALSATQNNSDSFAVCLACAMIQRSLERKNMSTPSQCSSCFEQYCWNGTTVNNPSAVSNYAPTVLSASTTSGTSSVRAKPIVFYLFASLLTVSLLL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must contain a signal peptide for proper functionality.2. The protein should have lysophospholipase activity, phospholipase A2 activity and be active in cytosol, cytoplasm, external side of plasma membrane, intracellular membrane-bounded organelle, extracellular region.3. The designed protein should contain one or more PLA2c domains that are essential for its function. The designed protein sequence is ",
    "response#1": "SVHNLRPADIKVIGALGDSLTAGNGAGSQPGDIIDVLTQYRGLSWSVGGNENITTVGTLPNILREFNPSLTGFSTGTGNHLTTNAFLNQAVAGARAEDLSSQARRLVDLMKNDTSINFQEDWKIITLFIGGNDLCGYCNDLVRYSPQNFSGNIRKALDILHEEVPRAFVNLVSVLRIDSLRELYQEEKVNCPKWLLSTICPCVVSSEDNSTKLAELIELNKQYQVATHQLVDSGRYDTKEDFTVVLQPFMEKVNMPKTTEGLPDTSFFAPDCFHFDRKTHARSARALWNNMLEPVG",
    "response#2": "SVHNLRPADIKVIGALGDSLTAGNGAGSQPGDIIDVLTQYRGLSWSVGGNENITTVGTLPNILREFNPSLTGFSTGTGNHLTTNAFLNQAVAGARAEDLSSQARRLVDLMKNDTSINFQEDWKIITLFIGGNDLCGYCNDLVRYSPQNFSGNIRKALDILHEEVPRAFVNLVSVLRIDSLRELYQEEKVNCPKWLLSTICPCVVSSEDNSTKLAELIELNKQYQVATHQLVDSGRYDTKEDFTVVLQPFMEKVNMPKTTEGLPDTSFFAPDCFHFDRKTHARSARALWNNMLEPVG",
    "response#3": "VCSSTDLRFSMALCDEEKKFRQQRKEKIMHKMKNLLGAENSKNLTSSRDVPVIAVLGSGGGFRAMVGFSGVMKALYESGILDCATYIAGLSGSTWYMSTLYSHPDFPRKGPKEINKELMNSVSHNPLLLLTPQKVKRYIEALWNKKRFGQPVTFTDIFGMLIGETLIHNRMNTTLSKMKEKINEAQCALPLFTCLHVKPDVSELMFADWVEFSPYEIGMAKYGTFMPPDLFGSKFFMGTVVKKHEENPLHFLMGVWGSAFSILFNRVLGVSNSHNKGPTMEEELENIRLKHLVGNDSSDSEDESQAPKGSENPEAENELQNSNQESWVQRMLMALVGDSSLFNTREGRAGKVHNFMLGLNLNTCYPLSPLSGLGTQESMEEDEMDAAVADPDEFERIYEPLDVKSKKIHIVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSSPPFKEILLAEKWAKMNNLPFPKIDPHVFDREGLKECYVFKPKNTATENDCPTIIHFVLANINFREYKAPGVPRQTQEEKDFADFDIFDDPNTPFSTFNFQYSNEAFKRLHDLMEFNTLNNLDVIKQAVVESIDYRRQNPSRCSVSLSNIEARKYFP"
  },
  {
    "reference": "MGTAGAMQLCWVILGFLLFRGHNSQPTMTQTSSSQGGLGGLSLTTEPVSSNPGYIPSSEANRPSHLSSTGTPGAGVPSSGRDGGTSRDTFQTVPPNSTTMSLSMREDATILPSPTSETVLTVAAFGVISFIVILVVVVIILVGVVSLRFKCRKSKESEDPQKPGSSGLSESCSTANGEKDSITLISMKNINMNNGKQSLSAEKVL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should be optimized for stability and expression in intracellular membrane-bounded organelle, plasma membrane where it will participate in positive regulation of proteasomal protein catabolic process. The designed protein sequence is ",
    "response#1": "QGCGELFEKAKQNNNNGEVSSGDNGLYFSNFSLFGTPVLKDISFSIERGQLLAVAGSTGAGKTSLLMMIMGELEPSEGKIKHSGRISFCSQFSWIMPGTLKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEVFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLRPDFSSKLMGFDSFDQFSAERRNSILTETLRRFI",
    "response#2": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPS",
    "response#3": "QGCGELFEKAKQNNNNGEVSSGDNGLYFSNFSLFGTPVLKDISFSIERGQLLAVAGSTGAGKTSLLMMIMGELEPSEGKIKHSGRISFCSQFSWIMPGTLKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEVFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLRPDFSSKLMGFDSFDQFSAERRNSILTETLRRFN"
  },
  {
    "reference": "MDRPPTDRPPQTQRSNSRIWLFLLLFVFIVLLLNSSAAPPTRDYSELETWLNTSFKPGNTSIDRGDIKDITIDQQTNHAIVVFKQTQNLPSAGGNTREFDKVQVVIPKSNGPSDLKRLLDLKFDPISENKIEYRYSPQDTTASVILFIAILLPIAILIFFWISYRRSQSQFMGGGFLSGFGKSNAKRYDQDEEPITFKDVAGQKAAKSDLQEIVEFLSSPDKFQKLGGRVPKGVLLNGPPGTGKTLLARAVAGEAGVPFFSINGSEFIQMFVGVGASRVRDLFQQAKESSPAIIFIDEIDAVGRQRGAGVGGGHDEREQTLNQILGEMDGFATNDSVIVVAATNRPDVLDPALLRPGRFDRHVTVGKPTQKGRRDIFKVHVRKVPLSNDVDLERLASGTVGLTGADIRNMVNEAALWAARHDKDCVEMEDFDYARDKILMGAKREEHLTAEDKEKTAYHEAGHTLASWFLKGADRVHKVTIIPRGRSLGSTQMLPEEDRVSMSETQLKDHLVVLLAGRAAEKLLYNETAVGAENDLERATSTARRMVTRWGMSEALGPVSYKLSDEDPFLGREIHEQRQFSEHTMEVIDSEVAKFLHEAEKRADELLVQYREELENLTEKLVESEELSEEQIADVIGPSVHPMPKPKEDNQGQIETIDDPATNDTAAGDDDTSAAESQSDSTPTDSNDVSTNNRITEGESGETDTAPQSF",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be optimized for ATP binding, zinc ion binding, metalloendopeptidase activity activity in order to improve proteolysis, cell division. The designed protein sequence is ",
    "response#1": "IPKGALLVGPPGTGKTLLARAVAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRSRGAGLGGGNDEREQTLNQLLVEMDGFESNEGIILIAATNRPDVLDPALLRPGRFDRQVVVPNPD",
    "response#2": "IPKGVLLVGPPGTGKTLLARAIAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQGKKNAPCLIFIDEIDAVGRHRGAGLGGGNDEREQTLNQLLVEMDGFEANEGVILIAATNRPDVLDPALLRPGRFDRQVVVPNPH",
    "response#3": "IPKGVLLYGPPGTGKTLLARAVAGEAGVPFFTISGSDFVEMFVGVGASRVRDLFEQAKENSPCIIFVDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFGDRQGIILIAATNRPDILDPALLRPGRFDRQIPVTNPL"
  },
  {
    "reference": "MDIDAYFERIGYKNSRDKLDLETLTDILQHQIRAIPFENLNIHCGEAMELDLEVIFDQIVRRKRGGWCLQVNHLLYWALTMIGFETTILGGYVYNTFNDKYSSAMIHLLLKVTIDGRDYIADAGFGRSYQMWQPLELISGKYQPQTPCIFRLTEDRGTWYLDQIRREQYIPNQDFLDSDLLEKNEYRKIYSFTLEPRTIKDFESVNTYLQESPASVFTSKSFCSLQTPEGVHCLVGFTLTYRRFNYKDNTDLVEFKTLNEKEIEENLKNIFNISLEKKLTPKHGDKFFTI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Participates in the detoxification of a plethora of hydrazine and arylamine drugs..2. The designed protein should have a binding site that is specific to the target CoA, substrate ligand.3. The protein should have a fast turnover rate for acetyl-CoA + an arylamine = an N-acetylarylamine + CoA.4. The protein can be expressed in cytoplasm and perform arylamine N-acetyltransferase activity without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "LTAARGRGKSAALGLAIAGAVAFGYSNIFVTSPSPDNLHTLFEFIFKGFDALQYQEHLDYEIIQSLNPEFNKAVIRVNVFREHRQTIQYIHPADAVKLGQAELVVIDEAAAIPLPLVKNLLGPYLVFMASTINGYEGTGRSLSLKLIQQLRQQSAQSQISTTAENKATTTARLASARTLHEVSLQESIRYAPGDHVEKWLNGLLCLLTAARGRGKSAALGLAIAGAVAFGYSNIFVTSPSPDNLHTLFEFIFKGFDALQYQEHLDYEIIQSLNPEFNKAVIRVNVFREHRQTIQYIHPADAVKLGQAELVVIDEAAAIPLPLVKNLLGPYLVFMASTINGYEGTGRSLSLKLIQQLRQQSAQSQISTTAENKATTTARLASARTLHEVSLQESIRYAPGDHVEKWLNGLLCLLTAARGRGKSAALGLAIAGAVAFGYSNIFVTSPSPDNLHTLFEFIFKGFDALQYQEHLDYEIIQSLNPEFNKAVIRVNVFREHRQTIQYIHPADAVKLGQAELVVIDEAAAIPLPLVKNLLGPYLVFMASTINGYEGTGRSLSLKLIQQLRQQSAQSQISTTAENKATTTARLASARTLHEVSLQESIRYAPGDHVEKWLNGLLCLL",
    "response#2": "VLTAARGRGKSTLAGMLVTHWQGACWVTGPGKAATQVLNQQAGERARFWAPDALLDYCQRHDVSDIDWLLIDEAAAIPTPLLSALLAYFPRALLTTTVQGYEGTGRGFLLKFCATLGDWHHLTLTDPIRWATDDPLERVMDNAMLR",
    "response#3": "RLMALYVASHYKNSPNDLQMLSDAPAHHLFCLLPPVPPTQNSLPEVLAVVQVCLEGEISRQSILNSLSRGKKAFGDLIPWTISEQFQDPDFGSLSGGRIVRIAVHPDYQGMGYGSRALQLLQMYYEGQFPCLEEKVIQKSQEINTVSSEAVSLLEEAVTPRKDLPPLLLKLSERPAENLDYLGVSYGLTPRLLKFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTQ"
  },
  {
    "reference": "MSHDGPKTIRVAAVQAEPEWNNLQKGVEKTIRLIIEAGKNGANVMGFPEVWIPGYPWSIWNQSVVDNVEFMDEYFRNSLERDSEEMNRIRCAVKEAGIFCVLGYSERYQGSLYISQSFIDENGDIVHHRRKIKPTHVERGFWGEGQADSLKSVVKSSFGNIGGLNCWEHTQTLLRYYEYAQNVDIHIASWPLIFGACAEMQYHISSEMCGKLTQVMSMEGACFTLICSQVMSAENCERNKVDKWSFVKAPGGGFSMIYGPAGEPLVEAPDAGEEVILYADVKLAEKWRAKQNLDVVGHYSRPDLLSLKVTNNAASQVHFA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein with the reaction a nitrile + 2 H2O = a carboxylate + NH4(+), the Proton acceptor, Nucleophile, active site must be optimized to ensure efficient catalysis.2. The protein must exhibit the following characteristics: Nitrilase that hydrolyzes preferentially benzonitrile and 4- cyanopyridine; Also acts on dinitriles like phenylenediacetonitriles (PDAs) 1,2-PDA, 1,3-PDA, and 1,4-PDA, and cyanophenyl acetonitriles (CPAs) 2-CPA and 4-CPA, but with lower activities..3. The protein must be able to indole-3-acetonitrile nitrilase activity. The designed protein sequence is ",
    "response#1": "RVAVTQAEPVWLDLDATVKKTCDLIVEAAANGAQLVAFPECWIPGYPAWIWTRPVDMRLSSTYIQNSLKIDSPQMASIQKCAAENKIVVVLGFSENLHNSLYISQAIIGSDGKILTTRKKIKPTHMERTIFGDSFGDCLQSVVDTSAGRVGALSCWEHIQPLLKYHTYAQREQIHVAAWPPLFPHNEDGSLFSMSSEGTSSIARTYAIESQSFVLHTTTVIGQSGVDRMATHGGALMSTPGGGCSAIFGPDGRQLSQPIPSTEEGIIYADLDLDQID",
    "response#2": "VRVAVTQAEPVWLDLDATVKKTCDLIVEAAANGAQLVAFPECWIPGYPAWIWTRPVDMRLSSTYIQNSLKIDSPQMASIQKCAAENKIVVVLGFSENLHNSLYISQAIIGSDGKILTTRKKIKPTHMERTIFGDSFGDCLQSVVDTSAGRVGALSCWEHIQPLLKYHTYAQREQIHVAAWPPLFPHNEDGSLFSMSSEGTSSIARTYAIESQSFVLHTTTVIGQSGVDRMATHGGALMSTPGGGCSAIFGPDGRQLSQPIPSTEEGIIYADLDECGNPALDELSVEITARVKALESMLIEQGILTTSMIDRMAEIYENEVGPHLGAKVVVKAWTDPEFKKRLLADGTEACKELGIGGLQGEDMMWVENTDEVHHVVVCTLCSCYPWPVLGLPPNWFKEPQYRSRVVREPRQLLKEEFGFEVPPSKEIKVWDSSSEMRFVVLPQRPAGTDGWSEEELATLVTRESMIGN",
    "response#3": "FRAAVVQAAPVPNDVEATIEKTINLIREAAARGANVAVFPEAFIGGYPKGANFNIHIGARTPEGRQEFADYRAGAIAVPGSETEQLAQAAHEAGLYLTIGVIERDGGTLYCTALYFTPDGLAGKHRKLMPTGAERLCWGFGDGSTLDTVQTPWGSMGAVICWENYMPLMRTAMYGKGIALYCAPTADDRDSWAATMRHIALEGRCFVLSACQYLTRKDFPESMGNRITDEPDAVLMRGGAIIVDPLGRVVAGPDYSGETILTADLDQASKPMFDYIEITARVKALESMLIEQGILTTSMIDRMAEIYENEVGPHLGAKVVVKAWTDPEFKKRLLADGTEACKELGIGGLQGEDMMWVENTDEVHHVVVCTLCSCYPWPVLGLPPNWFKEPQYRSRVVREPRQLLKEEFGFEVPPSKEIKVWDSSSEMRFVVLPQRPAGTDGWSEEELATLVTRESMIGS"
  },
  {
    "reference": "MTKYIFITGGVVSSLGKGIAASSLAAILESRGLSVTMTKLDPYINVDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFLNSKMRKVNNFTTGQVYENVLRKERKGEYLGATVQVIPHITDEIKRLIERSAEGYDVVLVEIGGTVGDIESLPFLEAIRQMRVERGDTNTLFIHLTLVPYIRTAGEIKTKPTQHSVKELRTIGIQPDILICRAEFPIPQAERGKIALFTNVEEKAVISAIDADTIYRIPLLLNEQGLDEIVTRKLHLDAKPADLSEWEAVTNAVLAPEKSVTVAMVGKYVQHSDAYKSLSEALSHAGIQTRTKVNIKYVESGELEENGITGLDDVDAILVPGGFGERGVEGKIATVKYARENGIPYLGICLGMQVAVIEFARDVAGMEGAHSTEFLKNTPYPVIGLITEWKDEAGQVEQRDETSDIGGTMRLGAQKASLIPGSLTHDLYGKDVITERHRHRYEFNNQYLEKLEAAGLKVSGKSIDGRLVEVIEIPDHPWFLACQFHPEFTSTPRHGHPLFTGFIQAAVEHSQKNEESAA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should have ATP binding, CTP synthase activity, glutaminase activity to facilitate 'de novo' CTP biosynthetic process.2. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates..3. The designed protein should contain one or more CTP synthase N-terminal, Glutamine amidotransferase domains that are essential for its function.4. The designed protein should be able to compete with endogenous proteins or inhibitors for ATP, Mg(2+) binding.5. The Nucleophile; for glutamine hydrolysis, Nucleophile active site of the designed protein must be optimized for substrate binding and specificity.6. We require a protein that can specifically bind to the metabolites produced in the Pyrimidine metabolism, CTP from UDP: step 2/2 pathway. The designed protein sequence is ",
    "response#1": "KHIFVTGGVVSSLGKGLTAASLGNLLAGRGLRVVMQKLDPYLNVDPGTMNPFQHGEVFVTDDGAETDLDIGHYERFLDINLSRAANVTTGQVYSDVIARERRGEYLGDTVQVIPHITDEIKRRMREQASVEPRPDVIISEIGGTVGDIESQPFIEAARQIRHELGRRNVFFVHVSLVPYMGASGEQKTKPTQHSVAQLRSIGIQPDALVLRSDRPVEAANRRKIALMCDVDEAAVVNAVDVASIYDIPSMLHSQDLDDYIVDHLGMPVPAADLSDWEAVLARVKEPTDTVTIGVVGKYIDLPDAYLSVVEAIRAGGFAHKTKVSIRWIASDDCATEEGAQKQLADLDGICVPGGFGIRGIEGKVGALRFAREHGIPTLGLCLGLQCMVIEYARNVAGLEGASSSEFDPDSPHPVIATMTEQVDIVSGNGDLGGTMRLGLYEASLQKGSVVRDLYGEDSIQERHRHRYEVNNSYRDALTAAGLVISGQSPEGNLVEFVELPAREHPFYVATQAHPELRSRPNRAHPLFVGLVDAALTRAASKDAYKSLGEALRHGGIKQNTAVHLKWLDSEQIEAEGAAAVLGDVDAVLVPGGFGKRGFEGKVQAAQYAREHKVPYFGICYGMHAAVVDFARHVAGIEGADSSENDRHAKDPVIALVTEWTTASGEVETRSEQSDLGGTMRLGAQEARLKPGTLAHNLYGRDVVRERHRHRYEFNNRYRQQFEDLGMVVSGKSMDDLLVELVELPPQTHPWFLACQAHPEFTSTPRDGHPLFIGFVKP",
    "response#2": "DAYMSIHESLKHAGIELGVEVDVNWVDAEKMRENHRERLRAADGVIVPGGFGVRGTDGKVEAVRHARENGVPFLGLCLGFQMAVVEHARNVLGLAGANSAELDPGTPHPVIDILPEQKELDDMGGTMRLGAHETEIEPGTLAAELYGDSCVERHRHRYEVNPEYIGRLESEGLVFSGTAGPRMEILERDDHPFFFGTQFHPEFRSRPDRASPAFVGFLDAYMSIHESLKHAGIELGVEVDVNWVDAEKMRENHRERLRAADGVIVPGGFGVRGTDGKVEAVRHARENGVPFLGLCLGFQMAVVEHARNVLGLAGANSAELDPGTPHPVIDILPEQKELDDMGGTMRLGAHETEIEPGTLAAELYGDSCVERHRHRYEVNPEYIGRLESEGLVFSGTAGPRMEILERDDHPFFFGTQFHPEFRSRPDRASPAFVGFLDAIEDFKRANLPDAYLSVTEALRAGGFAHRAKVEIKWVPSDECESPAGAQAALGDVDAVLIPGGFGIRGIEGKLGAVRFARTRKVPLLGLCLGLQCMVIEAARSVGLTDANSAEFEPDIEFPVISTMADQEDAVAGEADLGGTMRLGAYPATLLKGSVVAGAYGSTEVSERHRHRYEVNNTYRDRISKSGLVFSGTSPDGHLVEFVELPVSMHPFFVGTQAHPEYKSRPTRPHPLFAALVEAYKAAA",
    "response#3": "LPDAYLSVVEAIRAGGFAHKTKVSIRWIASDDCATEEGAQKQLADLDGICVPGGFGIRGIEGKVGALRFAREHGIPTLGLCLGLQCMVIEYARNVAGLEGASSSEFDPDSPHPVIATMTEQVDIVSGNGDLGGTMRLGLYEASLQKGSVVRDLYGEDSIQERHRHRYEVNNSYRDALTAAGLVISGQSPEGNLVEFVELPAREHPFYVATQAHPELRSRPNRAHPLFVGLVVRIALVGKYVELQDAYISVREALIHAALAHDRRVEIDWIQSEDLERGIGWDRLQRADGIVVPGGFGERGVPGKILAARFARENNIPYLGLCLGMQVMCIEFARHVLGSDEPNSTEFDPHTKHPVISLMPDQHAITDLGGTMRLGAYPCVLQPGTKAYAAYGEEVVYERHRHRWEFNNAYRDLLGEAGLVFSGLSPDGRLVEIVELRDHPWMVGSQFHPEFKTRLNRPHPLFRDFLAACGADIKRLTA"
  },
  {
    "reference": "MDKSQKNPTSQIGTSTPKSKFEDSPVFNYISNLSPIESVKSISTAQTFSSLSFTSPPPVFTSPHVISHRESRFFRCHNSVDRSKHLESLDGSAVKGEVVVPLVEDLNKEASLEDEEETSVETSSELPQIMKFDSQTSEHSDSPCTEDVVIEASSDPPRGDNGSSSEDVTMGLQNMLVVREGNDTPGCGRLISDATELLVFRSPNDSEAFRCLVDKISSSERRFCAGVKSTKRPDINKDIPANGSSNENQPLAVLPTNESVFNLHRGGMRRRCLDFEMPGKRKKDIVDDQQSVCDNNVAGESSSSCVVPGIGLHLNAVAMSAKDSNISVIHGYSISGEIQKSFSGSTTPIQSQDTVQETSDQAENEPVEEVPKALVFPELNLGSLKKKMRKSEQAGEGESCKRCNCKKSKCLKLYCECFAAGVYCIEPCSCIDCFNKPIHEETVLATRKQIESRNPLAFAPKVIRNADSIMEASDDASKTPASARHKRGCNCKKSNCMKKYCECYQGGVGCSMNCRCEGCTNVFGRKDGSLLVIMESKLEENQETYEKRIAKIQHNVEVSKEVEQNPSSDQPSTPLPPYRHLVVHQPFLSKNRLPPTQFFLGTGSSSFRKPNSDLAQSQNEKKPLETVTEDKTEIMPEILLNSPIANIKAISPNSKRVSPPQPGSSESGSILRRRGNGRKLILRSIPAFPSLNPNQ",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have transcription cis-regulatory region binding within nucleus to regulate regulation of meristem structural organization, guard mother cell cytokinesis, epidermal cell division.2. The protein must exhibit the following characteristics: Regulates the floral meristem cell division and the inflorescence meristem organization; Probable floral-specific cell division component, required for proper organ formation in flowers; Plays a role in development of both male and female reproductive tissues.. The designed protein sequence is ",
    "response#1": "MSGVNVGGDYVEVMCGCTSHRYGDAVARLRVFPTGDLEITCECTPGCDEDKLTPAAFEKHSGRETARKWKNNVWVIIGGEKVPLSKTVLLKYC",
    "response#2": "MSGVNVGGDYVEVMCGCTSHRYGDAVARLRVFPTGDLEITCECTPGCDEDKLTPAAFEKHSGRETARKWKNNVWVIIGGEKVPLSKTVLLKYL",
    "response#3": "MSGVNVGGDYVEVMCGCTSHRYGDAVARLRVFPTGDLEITCECTPGCDEDKLTPAAFEKHSGRETARKWKNNVWVIIGGEKVPLSKTVLLKYL"
  },
  {
    "reference": "MRVRGTRRNYQQWWIWAVLGFWMLIICKGGGNLWVTVYYGVPVWKEAKTTLFCASDAKAYEREVHNVWATHACVPTDPNPQEMVLQNVTENFNMWKNDMVDQMHEDVISLWDQSLKPCVKLTPLCVTLECRNVSLNRTDVNNSSAYNVSMEESMEVMKNCSFNTTTILRDKKKTEFALFYRSDIVPLNDKNSSKNSSEDYRLINCNTSAITQACPKVSFDPIPIHYCTPAGYAILKCNDKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEKEIIIRSENLTNNAKTIIVHLNQSVEIVCTRPHNNTRKSIRIGPGQTFYATGEVIGNIRQAHCNISAEHWNKTLQRVGSKLAEHFPNKTIIFAPSSGGDLEITMHSFNCRGEFFYCNTSGLFNGTYNGTDNAPTDNSSITIPCRIKQIINMWQEVGRAMYAPPIEGNITCKSNITGLLLVRDGGNTTGNETFRPEGGDMRDNWRSELYKYKVVEIKPLGVAPTGAKRRVVEREKRAALGAMFLGFLGVAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQTRVLAIERYLKDQQLLGIWGCSGKLICPTTVPWNSSWSNKSLNEIWDNMTWMQWDKEISNYTNTIYELLADSQIQQEKNEKDLLALDSWKNLWNWFDITKWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSLQTPIPNPGGPDRLRGIEEEGGEQDKGRSIRLVSGFLALAWDDLRNLCLFSYH",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design should include a YXXL motif; contains endocytosis signal motif to ensure proper function.2. The Extracellular topological domain of the protein should be designed to be flexible or rigid.3. The designed protein should have structural molecule activity within virion membrane, viral envelope, host cell plasma membrane to regulate fusion of virus membrane with host endosome membrane.4. Design the protein with a signal peptide incorporated.5. The designed protein must have at least one Retroviral envelope protein GP41-like domain.6. The protein must exhibit the following characteristics: The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWKDAKTTLFCASEAKAYDTEVHNVWATHACVPTDPNPQETHLANVTENFNMWKNDMVEQMHEDIISLWDQSLQPCVKLTPLCVTLNCSDYKHNVSNSTVSNSSGLLISNSSELLNCSFNVTTVIRDRTQKMSALFYRLDVVPITEEQNSTQNSNSYRLINCNTSAITQACPKVTFEPIPIHYCAPAGFAILKCNDEEFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVMIRSENLTNNAKVIIVQLNETVAINCTRPNNNTRKGIHIGPGKAFYATGDIIGDIRQAHCNVSGAEWNKTLQRVVTQLRKHFNTTIVFDRPSGGDLEITTHTFNCGGEFFYCNTSALFNSSWNSTGNRTGGNINDTITLPCKIKQIINMWQRVGQAIYAPPIPGVIRCNSTITGILLTRDGGTGTTNETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTEAKRRVVEREKRAVLGMLFLGVLSAAGSTMGAASLTLTVQARQLLSGIVQQQSKLLRAIEAQQHLLQLTVWGIKQLQARVLAVEAYLKDQQLLGIWGCSGKRICTTVVPWNNSWSNKSYNDIWDNMTWMQWDKEIENYTGQIYTLIEEAQIQQEKNEQELLELDKWASLWNWFDITKWLWYIKIFIMIVGGLIGLRIIFTVLSIVNRVRQGYSPLSFQTRLPAQRGPDRPEGIEEEGGDRDRDRSTRLVHGFLTLVWDDLRSLCLFSYHRLRD",
    "response#2": "LWVTVYYGVPVWRDADTTLFCASDAKAHETEVHNVWATHACVPTDPNPQEIQLENVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTNANLTHNIINGNSTIGNITDNITDEVKNCTFNMTTEIRGKQQKVYALFYTLDIVHMGGNDTEYRLINCNTSVIKQACPKVTFDPIPIHYCAPAGYAILKCNEKKFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKNIIVHLNKSVIINCTRPSNNTRMSTRIGPGQMFYRTGDIIGDIRKAYCEINGTEWNETLNQVTKKLKEHFNKTIIFQPPVGGDLETTMHHFNCRGEFFYCNTTKLFNSTNMEGFNTTIILPCRIKQIVKMWQGVGQAMYAPPISGIVNCISNITGILLTRDGGVNETFRPGGGNIKDNWRSELYKYKVVQIEPLGIAPTRAKRRVVNREKRAALGMLFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGLWGCSGKLICTTNVPWNSSWSNKSQGEIWDNMTWMQWEKEISSYSNEIYKLIEQSQNQQEKNEQELLALDKWASLWNWFSISNWLWYIRLFIIIVGGLIGLRIVFAILSIVNRVRKGYSPLSLQTRFPAPRGPDRPEGIEEEGGERDRDRSVRLVNGFLILFWDDLRSLCLFSYHRLRDLLLI",
    "response#3": "LWVTVYYGVPVWKEATTTLFCASDAKSYDTEVHNVWATHACVPTDPSPQEIRMENVTENFNAWKNNMVDQMHEDIISLWDESLKPCVKLTPFCVTLNCTKVNKTNPNTTWATVYYGVPVWRDAETILFCASDAKAHSTEAHNIWATQACVPTDPNPQEVLLPNVTENFNMWKNNMAEQMQEDIISLWEQSLKPCVQLTPLCVTMFCNNSNGEEPVDDLTQKNVTDIGDKRMKNCSFNATTDLRDKKKTVYSLFYEEDVVQISNNTYRLINCNTTVVKQACPKTSFEPIPIHYCAPPGFAIMKCNEGNFSGNGSCANVSTVQCTHGIKPVISTQLILNGSLNTDDIIIRNDSSGNLLVQWNKTVSINCTRPGNNTRGQVQIGPAMTFYNIERIVGDTRQAHCIVSKKDWTKMWNRTKEKIGSNKTFQARNKTEGDPEVTHLMFNCRGEFFYCNTSKLFDKNLLNKTNETIVLPCRIKQIVNLWTRVGKGIYAPPVRGNLTCNSNITGLILEYSGNSGNEMVYPSGGHMVNLWRQELHNYKVVSIEPIGVAPSKAKRHTVSREKRAALGAMFLGFLGAAGSTMGAASMTLTVQARLLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLSLERYLRDQQLLGIWGCSGKLICTTAVPWNTSWSNKSLDQIWQNMTWMQWEREIDNYTDLIYDLIEKSQNQQEKNEQDLLELDNWASLWNWFTITNWLWYIKIFIMVVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQ"
  },
  {
    "reference": "MKEDQVVQEESGFQDDEESLFQDIDLLQKHGINMADIKKLKSVGICTIKGIQMTTRRALCNVKGLSEAKVEKIKEAANKLIEPGFLTAFQYSERRKMVFHITTGSQEFDKLLGGGIESMAITEAFGEFRTGKTQLSHTLCVTAQLPGTGGYSGGKIIFIDTENTFRPDRLRDIADRFNVDHEAVLDNVLYARAYTSEHQMELLDYVAAKFHEEAGIFKLLIIDSIMALFRVDFSGRGELAERQQKLAQMLSRLQKISEEYNVAVFVTNQMTADPGATMTFQADPKKPIGGHILAHASTTRISLRKGRGELRIAKIYDSPEMPENEATFAITAGGIGDAKE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The ssDNA binding site should be located in a region of the protein that is accessible to the ligand.2. A protein that can perform ATP-dependent activity, acting on DNA, ATP-dependent DNA damage sensor activity in condensed nuclear chromosome to support spermatogenesis, gamete generation, male meiosis I, DNA recombinase assembly. The designed protein sequence is ",
    "response#1": "MXKGXDXXX",
    "response#2": "MXVXPXWXX",
    "response#3": "MKTF"
  },
  {
    "reference": "MNRFLLATRNPHKTREFSEILGEEFVVRDLSAEPDAPSIEETGTTFEENAILKAVAISKRVPGLVVADDSGLEVDALGGAPGIYSARYAGPDAKDNDNVARLLLELRKLSIERPYRARFRCVLAVARDGKLLDTFVGVIEGAIVESPRGIGGFGYDPVFLPDGLTETFGEMDPGEKNRISHRASAIRRLRAALLK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.2. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.3. The protein's Proton acceptor must be carefully engineered to ensure that the amino acids involved in dITP + H2O = dIMP + diphosphate + H(+) are properly positioned for optimal catalysis.4. A protein with ITP diphosphatase activity, ribonucleoside triphosphate phosphatase activity, XTP diphosphatase activity, dITP diphosphatase activity functions is required to enable nucleotide metabolic process, purine nucleoside triphosphate catabolic process.5. The substrate, Mg(2+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MKIVLATHNRHKLEEIRDILPEHIELLSLKDIGLPDAPEDEDTLEGNAAKKAVDVWQATGLDCFADDTGLEVDALNGEPGVYSARYAGEQRNADDNIRKLLTNMRGIKNRKARFRTVISLILADGEKSVFEGIVNGTITDAPRGEKGFGYDPVFQPEGENRTFGEMSMEEKNAISHRGRAVRKLAEFLKNGGK",
    "response#2": "MKILVVAAGRGTRLKEHTNNTPKPMVKIGNKTILEHNIKILSQKNIFDIIIVTGHSKDTIKKYLEDKKYIIINGGDTRQNSIYNALNKIKDNTDYVLLEDVARPCLSKNLIDNVINNLEKYDACTSCIKVKDTIIKVNNNTVIETVNRSDLFNVQTPQCFKTNILKKTYKKTKKNNIKLTDDASLVEKSGIKINLIEGDKRNIKITTEDDLAIIENFINSNINC",
    "response#3": "MIDLTSIAXGTXVXGXDXRXAXGXVMQXTYXPGXDXVXGXXDXXA"
  },
  {
    "reference": "MGISRDSWHKRYKTGATQPVPHKKRKFELGRPAANTKIGAHRVRLVRTRGGNEKYRALRLDSGNFSWASEQTTRKTRIVDTMYNATNNELVRTKTLVKGAIISVDAAPFRQWYEAHYALPLARKKNAKLSEEDNAILNKKRSHHTMKKYTERQKTAAVDALLIEQFNTGRLLARISSSPGQVGQANGYILEGKELDFYLRKIRAKKAK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should have a specific function within the cytosolic small ribosomal subunit. The designed protein sequence is ",
    "response#1": "GAERPLKKPKDFTKAIGKNVYIKTYEPIEGEKEFEGELIGFDGTTVSVTVKDKTRKKTIDIPYEKVASARLAVIG",
    "response#2": "GAERPLKKPKDFTKAIGKNVYIKTYEPIEGEKEFEGELIGFDGTTVSVTVKDKTRKKTIDIPYEKVASARLAVIF",
    "response#3": "GAERPLKKPKDFTKAIGKNVYIKTYEPIEGEKEFEGELIGFDGTTVSVTVKDKTRKKTIDIPYEKVASARLAVIS"
  },
  {
    "reference": "MNLRPEEISSIIKEQIKRYENKLEVKDVGTVIQVGDGIARIHGLEKCMAGELLEFPNAVYGMAQNLEEDNVGCVLLGSDATIREGDIVKRTGRIVEVPVGEALLGRVVNALGQPIDGKGPINTDAYREVERVAPGIISRKSVHEPLQTGIKAIDSMIPIGRGQRELIIGDRQTGKTALAIDTIINQKNTDVICIYVAIGQKKSTVAQIKDSLEKAGAMEYTIIVSSTADELAPLQYLAPYAGCAMGEEFMEKGKHVLIIYDDLSKHAVAYRAMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDERGGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLETELFNAGIRPAVNPGISVSRVGGNAQIKAMKKVAGTLRLELAQYRELAAFAQFGSDLDKETQERLSQGERILEILKQPQYDPMPVEKQIMMIYATTKKYLTDIAVEDIRDFESGFLRFMDNEHPEVGKDIVATGSISEETEAKLKEAIESFKKQFKAERE",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be designed to have high stability and activity towards ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate.2. For general function, the protein need meet that Produces ATP from ADP in the presence of a proton gradient across the membrane3. The protein must have high specificity towards proton-transporting ATP synthase activity, rotational mechanism, ATP binding.4. The protein should have a high affinity for ATP. The designed protein sequence is ",
    "response#1": "MKQVAGMLRIDLAQFRELETFAQFASELDPATRAQIVRGQHLQELLKQDQYSPLPVEEQVVVLYAGVRGYLDELPVESVRKFEKEFLDYMRTSKSSLLKLISEKKELTQEVEDELKKSIQEFLEGR",
    "response#2": "RGGKAGLFGGAGVGKTVLITELIHNMVGQHEGVSLFCGIGERCREGEELFREMREAGVLDNTVMVFGQMNEPPGARFRVGLTALTMAEYFRDDQGRDVLLLIDNIFRFIQAGMEISGLLGLMPSRLGYQPTMGTELAELQERISSTRHAAITSIQAVYVPADDFTDPAAVHTFSHLSASIVLSRRIKGGKVGLFGGAGVGKTVNMMELIRNIATEHSGYSVFTGVGERTREGNDFYHEMSDSRVLDKVSLVYGQMNEPPGNRLRVAFTGLTIAEKFRNEGHDVLLFIDNIYRYTLAGTEVSALLGRIPSAVGYQPTLSEEMGVLQERITSTNKGSITSIQAVYVPADDLTDPSPATTFSHLDSTITLSRQIVSLGIYPAIDPLNSTSRQLDPRIVGQLHYDVALGVRSILQRYQELKDIIAILGMDELSEDDKILVSRARKIQKFLSQPH",
    "response#3": "KGGKVGLFGGAGVGKTVNMMELIRNIAIEHSGYSVFAGVGERTREGNDFYYEMEESGVLDKVALVYGQMNEPPGNRLRVALTGLTMAEYFRDEGRDILFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLSQEMGQIQERITSTKTGSITSIQAVYVPADDLTDPAPATTFAHLDATVVLNRSIS"
  },
  {
    "reference": "MDKLTPEQRKKCMKASSKNKGTKPELLLAKYLWALGLRYRKNDRSIFGTPDLSFKRYKIAIFIDGEFWHGKDWDIRKYDIKSNKDFWISKIEHNMNRDKKVNDYLISNGWVIFRFWGKDVLKNPEKFSLEIQKAIYERCVR",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: If unrepaired, these mismatches can lead to C-to- T transition mutations; This enzyme is an endonuclease that nicks double-stranded DNA within the sequence CTGG next to the thymidine residue that is mismatched to 2'-deoxyguanosine; The incision is mismatch-dependent and strand-specific.; May nick HpaII sequences that contain T/G mispairs resulting from m5C-deamination; The very short patch (VSP) repair process counteracts the mutagenic process by repairing the mismatches in favor of the G-containing strand.2. The designed protein should have endonuclease activity to facilitate mismatch repair. The designed protein sequence is ",
    "response#1": "PELPEVETALRGISPYLKNFTIEKVVVRQPKLRWAVSEELITLKNVKIVDLTRRAKYLIIHTEKGYIIGHLGMSGSVRIVPQDSAIDKHDHIDIVVNNGKLLRYNDPRRFR",
    "response#2": "PELPEVEVVRRGLHHHLVGKTIASTLVYHERAVRRQSGGAVELAGLLAGQQISGTGRRGKYLWLTLEGSSGAQALVVHLGMSGQMLIGPISRPQHLRIAATLDDGSVLSFVDQRTFGGWIGE",
    "response#3": "PELPEVETIKETLKLFVCNKTIKHIDIEWPNMIKHPDDVEEFKALVTGQTIRSMGRKGKFLLFYLDEYVLISHLRMEGKYSVHSPGDPVKKHTHVTFYFSNGEELRYNDVRKFGTMV"
  },
  {
    "reference": "MNLIPTVIEQTNRGERAYDIYSRLLKDRIIMLGSGVDDNVANSIVAQMLFLQAEDPEKDISLYINSPGGSITAGMAIYDTMQFIKPDVSTICIGMAASMGAFLLTAGAKGKRMALPNSEVMIHQPLGGMQGQAADMEIHARRIIQMREKLNQIMAERSGQPYERIARDTDRDNFMTAEQAKEYGLIDKVIETPTK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A key design requirement for the protein is the optimization of the Nucleophile, active site to achieve high catalytic efficiency for Hydrolysis of proteins to small peptides in the presence of   ATP and magnesium. alpha-casein is the usual test substrate. In the   absence of ATP, only oligopeptides shorter than five residues are   hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-   Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also   occurs)..2. For general function, the protein need meet that Plays a major role in the degradation of misfolded proteins; Cleaves peptides in various proteins in a process that requires ATP hydrolysis; This results in the release of SigW and the transcription activation of the genes under the control of the sigma-W factor .3. A protein that contributes to proteolysis by functioning in cytoplasm with serine-type endopeptidase activity, ATP-dependent peptidase activity. The designed protein sequence is ",
    "response#1": "MQAEMPAHSHDFHIHAHAHAHEPRPGGGAPTPSPPTPGAPTPGAPTPGAPTPGAPTPGAPTPGAPTPGAPTPGAPTPGAPTPGAPTPGAPTPGAPTPGTPPRPAPPRPRPLDVFLGIGPGEELEPLPPPRRRRIALTLVGGVVLGLLVLGAGYLLQAAPLDVLRGRQRAEQWAQVQAALGEWGSWAVAAVIVLLVVQALVSPLPLELGVLAGFVYGTLWGTVLAWAGTVLGSALVFLLARAWLRDWVVRRVGSARLGRLDDLVGRRGVLAVFLTRLVPVLPFNAVNYLAGLSRVRFRDYVTATLIGMLPGTLLYTALGSGLASVRSGAADPRAVAAVAAGVLVMAGLVALYVALDRRVRAAVEEGALEGAR",
    "response#2": "MRYITPNARVLLHQEADAARHAADWLQQQGAEVTLCHSQTHLNVADNSGARQAWQQLADEVKG",
    "response#3": "MERYITPNARVLLHQKTHFCEQIFPCLQDKTSDSQPSQPTQTNSPQASTSTSTAQSTSATSQPQCPGFTPVGFKWSDPQKLREFKKLVKERYPPIPSISDWKNASPEERLQALQKFKEEFQVPPKACWNHPPLSDEVRDRLNKLRAENPKYSKFMSQYGKYQYYRQRKGKMKENPDQPPEPPKSPKAPSKPPSAPQPGPAGKKPKPPAKSPDQSPKPKKLPNQPPKSPKSPPKLPKSPSKPPKGQKKPPGQPPKPPKPKAKPSKPAKPAKPAKPAKPAKPAKPAKPAKPAKAAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKPAKAPAKKK"
  },
  {
    "reference": "MEARVAEAAARLARQENEIKSLTAEIEHLKNFGCLGISPSLEGLRDENAKLKYRLNFLQKSLQEEQSKTVKSMININSRLQEIFGAAIQAAYPELENPPLVVTPSQQPKFGDYQCNSAMGITQILLKTKEQKVSPREIAEKITKHIPANECIEKVEIAGPGFINVHLRKDFVSKQLSSLLVNGVQPPAIGKRKKVILDFSSPNIAKEMHVGHLRSTIIGESMCRLFEFAGYDVLRLNHLGDWGTQFGMLIAHLQDRFPDYLTVSPPIGDLQAFYKESKRRFDTEEEFKKRAYQCVVLLQSKDPDFIKAWELICDVSRKEFQKIYNCLDVTLTERGESFYQDMMKDIVKEFEDKGFVQVDDGRKIVFVPGFSVPLTIMKSDGGYTYDTSDLAALRHRLLEEKGDILIYVVDSGQSVHLQTVFAAGQMIGWYDPKVTRITHAAFGVVLGEDKKKFKTRSGDTVRLMDLLEEGLKRAMDKLKDKERDKVLTPEELKAAQMSVAFGCIKYADLSHNRLNDYVFSFDKMLDDRGNTAAYLLYAFTRIRAIARLANIDEGMLRKAAREEVIVLDHEKEWKLGKCILRFPEILQKILDDLLLHTLCDYLYELATTFTEFYDNCYCVEKDRQSGQIMKVNTWRLLLCEATATIMAKGFDILGIKPVERM",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein can be expressed in cytosol and perform ATP binding without triggering undesirable cellular responses.2. The designed protein should contain at least one 'HIGH' region motif to achieve its intended function.3. The protein must exhibit the following characteristics: Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis.. The designed protein sequence is ",
    "response#1": "LQDFISRVNSDLVGKYVNIAARASGFIAKRFEGRPKDVSGSALLAKLAAESDTIAEQYENREYARALRDIMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAANAARFR",
    "response#2": "LQDFISRVNSDLVGKYVNIAARASGFIAKRFEGRPKDVSGSALLAKLAAESDTIAEQYENREYARALRDIMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAANAARFR",
    "response#3": "LQDFISRVNSDLVGKYVNIAARASGFIAKRFEGRPKDVSGSALLAKLAAESDTIAEQYENREYARALRDIMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAANAARFR"
  },
  {
    "reference": "MSSTRRMQILRAVVEDYIRQQEPIGSGALAAKHHLGVSPATIRNDMAALEDEGYLTQPHTSAGRIPTEKGYRYFVNSLSTPIPLTAEQRESISSALSGSASLQDTLQRAARILSTITGQYAMVSAPSLSRSLMRHVELLPLAAHTLLLVTITETGRVVQRLVSTDTLPNTEQTQCICETINTHCRLLTFRQCAQSIRTLPTDSEGGFTPQLRDAIAAVFSDMENEERPNELFTSGAASLTHQHIDAASLAPLFDALEEQVVLMRLMYDLTGQPGQEGVGVAIGSETHTPGLLHASVVSSGYGQSSTPSANVEHEEYDTGTKPTMPIAVIGSIGPTHMNYAVTMAAVHAVSRYLTEFLRVGHNGWQE",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must be able to regulate negative regulation of DNA-templated transcription.2. The protein must exhibit the following characteristics: Prevents heat-shock induction of these operons.; Negative regulator of class I heat shock genes (grpE-dnaK- dnaJ and groELS operons). The designed protein sequence is ",
    "response#1": "EHMLTEASQMLSGMSRGAGLVLAAKNEVALKHIEFIQLEPTKALAVLVSQNGDVENRVVELPAGITVSQLHEASNFLNAHIRGRTLAEARTEIARIKEETRAALDTLSQDLVEKGLAVWAGAESGLPARLIVRGRANLLENVTAQADIELLRHLFEDMETQDGLIQLLDLAEQGSGVRIFIGSENKLFSLSGSSLVVAPYRDKDARVVGALGVIGPTRLNYARIL",
    "response#2": "ILRRAANLLSGHARQLGMVVAPSEDEARWRSIEFAPAAEGLVLAVLMLEGGLVRTRTVRVDERYGQDELVRFGNYLNEHFRGLSLSEARDRIGHELARAGSRLEEMCVRALALSRRAVEHMGDDRELIVNGTLNMLDHAEFTDVGRMRDLLAAIEERSRLLELLDRTLSERDVRITFCQDVADGAPDGLRGCSVISAAYGGDTPRGVVSVVGPLRMDYAKIR",
    "response#3": "WLKLAASVLAETSGNVGLVTPPRARVERLRHFELISLRRRVVLLVLVTQSGTIHQSLLELPQPLEQEELSALSQRLNPELRWLDRAAIERRAQGAEPTVALVLQRLAEALHFIEHQQRSELYAEGLEHVIRQPEFAQANLAHLLLEVLRGGMLLTWLLPRLDPSEEVRVLIGSDLALRELRPFSLILTAYRSGAESTGWLGVLGPQRMAYARAL"
  },
  {
    "reference": "MRRSHLLATVACATLACAPLAANAQFAPAGSGGSPTSSVPGPGNGSGNSFEPTENTPAAKSRFSGPSPYAPQAPGVNAANLPDIGSMDPNDVPQMAPQQSASPASGDWAAYGHDDSQMRYSPLSEITPQNADQLKVAFVYHTGSYPRPGQTNKWAAETTPIKVGDGLYMCSAQNDIMKIDPATGKEIWRHNINEKYEAIPYTAACKGVTYFTSSQVPEGQPCHNRILEGTLDMRLIAVDAATGNLCEGFGNGGQVNLMQGLGESVPGFVSMTTPPPVVNGVVVVNHEVLDGQRRWAPSGVIRGYDAESGKFLWAWDVNRPNDHSQPTGNNHYSRGTPNSWAAMTGDNALGLVYVPTGNSASDYYSALRSPEENKVSSAVVALDVKTGSPRWVFQTVHKDVWDYDIGSQATLMDMPGQDGQPVPALIMPTKRGQTFVLDRRDGKPILPVEERPAPSPGVIPGDPRSPTQPWSTGMPALRVPDLKETDMWGMSPIDQLFCRIKFRRANYTGEFTPPSVDKPWIEYPGYNGGSDWGSVSYDPQSGILIANWNITPMYDQLVTRKKADELGLMPIDDPNYKPGGGGAEGNGAMDGTPYGIVVTPFWDQYTGMMCNRPPYGMITAIDMKHGQKVLWQHPLGTARANGPWGLPTGLPWEIGTPNNGGSVVTAGGVVFIAAATDNQIRAIDEHTGKVVWSAVLPGGGQANPMTYEANGHQYVAIMAGGHHFMMTPVSDQLVVYALPDHKG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The desired protein should be able to perform A + glycerol = AH2 + dihydroxyacetone2. The protein design should emphasize Polar residues compositional bias.3. The designed protein must be able to perform glycerol dehydrogenase (acceptor) activity under specific conditions. The designed protein sequence is ",
    "response#1": "STYGTGCFALLNTGATPVASKNKLLTTIAYQLNGKRTYALEGSIFVAGSAVQWLRDGLGLIKQASETGPLADRSDATQSVYLVPAFVGMGAPYWNPNVRGALFGLTRNTGPAELAHAALESVCYQTFDLWAAMRADWPDAAAAHTVLRVDGGMTASDWTMQRLADLLDAPVDRPVIQETTALGAAYLAGLVTILGAGSYGTALAIALSRNGYPTYLWGHNPTACAQMAQERQNARFLPDISFPEALRVESDLKSAVEKSKDLLIVVPSHVFGEVIQQIKPFLHNRHRIIWATKGLERGTGRLLQNLVEQELGSQYPLAVLSGPTFAKELAAGLPTAITLAAENEQFG",
    "response#2": "STYGTGCFALLNTGATPVASKNKLLTTIAYQLNGKRTYALEGSIFVAGSAVQWLRDGLGLIKQASETGPLADRSDATQSVYLVPAFVGMGAPYWNPNVRGALFGLTRNTGPAELAHAALESVCYQTFDLWAAMRADWPDAAAAHTVLRVDGGMTASDWTMQRLADLLDAPVDRPVIQETTALGAAYLAGLVTILGAGSYGTALAIALSRNGYPTYLWGHNPTACAQMAQERQNARFLPDISFPEALRVESDLKSAVEKSKDLLIVVPSHVFGEVIQQIKPFLHNRHRIIWATKGLERGTGRLLQNLVEQELGSQYPLAVLSGPTFAKELAAGLPTAITLAAENEQFLGLGR",
    "response#3": "STYGTGCFALLNTGATPVASKNKLLTTIAYQLNGKRTYALEGSIFVAGSAVQWLRDGLGLIKQASETGPLADRSDATQSVYLVPAFVGMGAPYWNPNVRGALFGLTRNTGPAELAHAALESVCYQTFDLWAAMRADWPDAAAAHTVLRVDGGMTASDWTMQRLADLLDAPVDRPVIQETTALGAAYLAGLSGVGFESGGVAAAHAVHNGLAAGAY"
  },
  {
    "reference": "MGGKWSKSSIVGWPAVRERMRRTRPGAAEGVGAASQDLDKYGALTSSNTASNNADCAWLEAQEEANEVGFPVRPQVPLRPMTQKGAFDLGFFLKEKGGLDGLIYSRKRQEILDLWVYHTQGYFPDWQNYTSGPGVRYPLTFGWCYKLLPVDPKEVEEASEGENNCLLHPMNQHGMEDEHGEVLMWKFDSQLARRHMARELHPEYYKDC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein must exhibit the following characteristics: Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1; Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis.2. A protein that can perform SH3 domain binding in membrane, extracellular region to support suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I, suppression by virus of host autophagy. The designed protein sequence is ",
    "response#1": "MPGKLTI",
    "response#2": "MFKL",
    "response#3": "LPLLNK"
  },
  {
    "reference": "MINGKNILLGISGSIAAYKTPYIVRELVNYDCNVKVILTKNATNFVSELTLSVLTKNKVNIEMVNDDKSWNSHVKLAEWADVFLIAPASSKTLSNLATGKCENLLIATFLSYNSFVFIAPAMDAEMNEHVTTNNNIKNLVSQGNFIIDSEFGELASGLSGFGRLSEPKKIIDHVIKTLSSDLPLSGTVSLVTAGPTRESIDSVRYLSNRSTGLMGLTIAKELARNGSEVNLILGPSPYKINYPNVNLIPVESCDEMYIEALKFFSKSDICVFSAAVSDYKISNKFSRKIKKSDFINLELEKNIDIAKELCKNKNEKQLCVGFALEDDNNIKNAQKKMKDKNFNFIVLNSLENKDTCFESFKNKITIIDNHNTFNFDLKSKKSVAKDIVTHIIKTK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a binding site that is specific to the target CTP ligand.2. The presence of Mg(2+) is necessary for the protein to function in the desired environment.3. The presence of Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.4. The designed protein should interact with enzymes and substrates within the coenzyme A biosynthesis pathway to enhance or regulate its activity.5. The designed protein should have FMN binding, metal ion binding to facilitate pantothenate catabolic process. The designed protein sequence is ",
    "response#1": "MEGCRLLVTAGPTREEIDPVRYLSNYSSGKMGYAIAAVAARRGARVTLISGPCTLSVPPGVERVLVTSALDMHGAVMEAADTADVVIKAAAVADYRPVNRAPQKVKKGDGEQLTLTLTRNPDILADLGTEKGGRLLVGFAAETDDLLKHAGEKLQNKNLDLIVANDVTCEGAGFDVETNIVRLFDKDGLVAEFVAG",
    "response#2": "MEGCRLLVTAGPTREEIDPVRYLSNYSSGKMGYAIAAVAARRGARVTLISGPCTLSVPPGVERVLVTSALDMHGAVMEAADTADVVIKAAAVADYRPVNRAPQKVKKGDGEQLTLTLTRNPDILADLGTEKGGRLLVGFAAETDDLLKHAGEKLQNKNLDLIVANDVTCEGAGFDVETNIVRLVDRDGREETLPLLSKDEAARYILEAA",
    "response#3": "MEGCRLLVTAGPTREEIDPVRYLSNYSSGKMGYAIAAVAARRGARVTLISGPCTLSVPPGVERVLVTSALDMHGAVMEAADTADVVIKAAAVADYRPVNRAPQKVKKGDGEQLTLTLTRNPDILADLGTEKGGRLLVGFAAETDDLLKHAGEKLQNKNLDLIVANDVTCEGAGFDVETNIVRVFDARGAISVTERTGYIGKVDALAKRIAADFVETRDKLGM"
  },
  {
    "reference": "MQKSSMLKKEVAIARRQWYLVDATDLVLGRLSVKVADILRGKNKVDYTPNVDAGDYVVVINSDKVVLTGQKALRENWYNHSHYIGGLRTRSGEEMISKYSDELIRRSVKGMLPKNKLAKQILNKLFIYKNDKHPHEAQQPTILELKLK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself; It is important during the early stages of 50S assembly..2. The protein should be optimized for stability and solubility when expressed in ribonucleoprotein complex, ribosome where it will be involved in translation. The designed protein sequence is ",
    "response#1": "MPPSPL",
    "response#2": "GEGEFYYHQIIGMAVYEKDVLIGHVKEILQPGANDVWIVKRQGKRDLLLPYIPPVVLNVDVPNKRVDVELMEGL",
    "response#3": "MNVGKIVNTQGLQGEMRVLSVSDFAEERFKKGSQLALFDDKDRFVQEVTIVSHRKQKHFDIIKFKDMYHINAIEKYKGYTLKVSKL"
  },
  {
    "reference": "MKNLFGVTREYLEEYFLSSGEKKFKATQVFEWLYQHKEWDITKFSNIKKEIQEKLMSDFDTSFIKIEIVEEGTLVKKFLFRLLDGEKIEAVLMEHDYGLSVCVSSQVGCNMGCRFCESGRLKKVRNLETYEIVEQILLIEKYVGKRIDSVVMMGIGEPFDNYDNIINFIKIINDAKGLAIGARHITVSTCGLVPKIKEFSELDLQVNLALSLHAPSDEVRNKIMPINKAYNIDTVIHAIKDYIAKTNRRVTIEYVMLNMVNDNKEDALLLAKLLRGMNVYVNLIPYNETNNIDFSKSDKKRIDIFYNTLKENGINVTVRREFGGNIKAACGQLRSESD",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The desired protein should be able to perform adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine The designed protein sequence is ",
    "response#1": "GGDRLTVCFSTQAGCAIGCAFCATGREGFARNLTPGEIIDQVLIAQDDMGKRVTNVVGMGQGEPFLNYGNTMAALRILNNKKGLEIGARHISVSTCGILPGIERFSEEPEQFTLAVSLHAARQGVRDLLMPNVARFKLGNLKSALQEYIAKTNRRVTLEYIMIDGVNDADEDLKALQKFCANLLCHVNLIPINAIEGSVFQPSEPETIDRWLNAIQKKGTEATLRDSRGSRR",
    "response#2": "SSQVGCSMGCKFCLTARSGFKRNLETWEIISQVVLAKRWLKERGESLPLRNIVFMGMGEPLANYTNLVQSLKILADHEGFNFSRKRLTVSTAGIVPKIWALAKDFPTALAVSLHSADQSLRDYLMPGVKQYPLSDLRESIKAFPRIKNGRTTIEYILLKDINDGEEHY",
    "response#3": "SSQVGCSMGCKFCLTARSGFKRNLETWEIISQVVLAKRWLKERGESLPLRNIVFMGMGEPLANYTNLVQSLKILADHEGFNFSRKRLTVSTAGIVPKIWALAKDFPTALAVSLHSADQSLRDYLMPGVKQYPLSDLRESIKAFPRIKNGRTTIEYILLKDINDGEEHYK"
  },
  {
    "reference": "MQSDIYHPGHSFPSWVLCWVHSCGHEGHLRETAEIRKTHQNGDLQIRGGRGRRESTEIFQVASVTEGEESPPAICMEVFLFLWFIAPIYACVCRIFKIQVRNTVKNSSTASLAPSISTSEERQIRIERHHYHLYGQ",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization.2. The designed protein should have a specific function within the membrane. The designed protein sequence is ",
    "response#1": "KWNNISTSFVHSLITGVWSVLCFCMHPQMAEDLIETHSVFSHALVSVSIGYFIYDFLDMVINQKIIHSWELLFHHVVVITCFGISVLTCRYVGFAVVALLVEINSVFLHLRQVLRMANLAKSTFYRVNSMINLGTYVVFRINTLAWMTRWLVLNRDLIPLFSYTIGSVGLAIMTAMNIVLFYRLMF",
    "response#2": "KWNNISTSFVHSLITGVWSVLCFCMHPQMAEDLIETHSVFSHALVSVSIGYFIYDFLDMVINQKIIHSWELLFHHVVVITCFGISVLTCRYVGFAVVALLVEINSVFLHLRQVLRMANLAKSTFYRVNSMINLGTYVVFRINTLAWMTRWLVLNRDLIPLFSYTIGSVGLAIMTAMNIVLFYRLMY",
    "response#3": "KWNNISTSFVHSLITGVWSVLCFCMHPQMAEDLIETHSVFSHALVSVSIGYFIYDFLDMVINQKIIHSWELLFHHVVVITCFGISVLTCRYVGFAVVALLVEINSVFLHLRQVLRMANLAKSTFYRVNSMINLGTYVVFRINTLAWMTRWLVLNRDLIPLFSYTIGSVGLAIMTAMNIVLFYRLMF"
  },
  {
    "reference": "MNLLPKTREEFAQTDYWNEFFKKRGEKAFEWYGEYLDLCDHIHKYIKPVDKILMLGCGNSKLSMDMYDSEYRDITNIDISPVAVKKMLEQNARTRPDMKFLQMDATAMTFPDESFSVALDKGTLDALFVDDAPETKAVVENYFKEILRTMRNGGRYFCVSLLQEHILNFLVEFLPRHNCMLRIVHCLGVEQANKEKNADDAMKMPVFVVIATKFKSLPMPILEFGLGNDKMQRFTESSELSNAVRSVQKAALVFNGLARSSIAGHDEVTLDLYRPSENTPRYSIYILDQAAARGLNKYAAFIVPQGREIEWLFGTPSGRKKLQASAKFQRLAVVTLHRDQVYNTLEEVQAELGDTVFSLAPHGHIKQIPYLSLGSDVGKRETLISGFSKISGEFRIEEVEAGGKTLRRLIFLSNQFVVQSEALVKTIKIKGKKERKKIDFGYLACQHHLYMSVGVQLATTLQNPKKDVQKDVLVIGLGGGGLCSFLHAALPQSRITAVEIDPIMLEVAEQYFELKQDKRFHVVIDDGLAFVERCRNEDIHFDAVLFDVDSKDLSLGMSCPPQGFLAHDVLLHIKEIIGPKGLFMLNLVCRDETLKTEAIANLQKVFPAVCSYKLEEDINEVVYCANDEKYKTVEHWQKAMGTAGRGLNTTIKEHKLASEDPLEVAEFLSELKL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein have enhanced N-terminal glycyl-L-lysyl-L-glutamyl-[protein] + 3 S-adenosyl-   L-methionine = 3 H(+) + N-terminal N,N,N-trimethyl-glycyl-L-lysyl-L-   glutamyl-[protein] + 3 S-adenosyl-L-homocysteine.2. For general function, the protein need meet that It catalyzes N-terminal methylation of target proteins via its C-terminus The designed protein sequence is ",
    "response#1": "DKILMLGCGNSKLSMDMYDSEYRDITNIDISPVAVKKMLEQNARTRPDMKFLQMDATAMTFPDESFSVALDKGTLDALFVDDAPETKAVVENYFKEILRTMRNGGRYA",
    "response#2": "DKILMLGCGNSKLSMDMYDSEYRDITNIDISPVAVKKMLEQNARTRPDMKFLQMDATAMTFPDESFSVALDKGTLDALFVDDAPETKAVVENYFKEILRTMRNGGRYH",
    "response#3": "DKILMLGCGNSKLSMDMYDSEYRDITNIDISPVAVKKMLEQNARTRPDMKFLQMDATAMTFPDESFSVALDKGTLDALFVDDAPETKAVVENYFKEILRTMRNGGRYR"
  },
  {
    "reference": "MLIIPAIDIKDGKCVRLTRGDFSQQKIYLDNPLDMAIIWRKQNAKMLHIVDLDAALTGEMVNFDIIANIVAELDIPVQIGGGIRSIDAVKRYLDMGVGRVVIGSAAVTDPELVGELMKHWPASKIVVGIDAENGVPKIKGWTESSGMQDYELALRMKDMGVERIVYTDISRDGMMQGFGYESTKRFAQKAGLKVTASGGVTNSEDLQRLATLRPYGVDSVIIGKAFYECNFPCQELWYNFEKGICLDQNFSTARRK",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must be optimized for 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity.2. Ideal protein should have a binding affinity for key enzymes in the Amino-acid biosynthesis pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "MKAVIIMGSKSDLDYSKKIASKLADFGIDAVMRIASAHKTPEKVLEIIKEYEKEDVVFVTVAGRSNALSGFVDANTSKPVIASPPYSDKFGGADIFSSIRMPSGVAPMLVLEAENAALAVAKIFALKDEGVREKVVQFQENKRREK",
    "response#2": "MKAVIIMGSKSDLDYSKKIASKLADFGIDAVMRIASAHKTPEKVLEIIKEYEKEDVVFVTVAGRSNALSGFVDANTSKPVIASPPYSDKFGGADIFSSIRMPSGVAPMLVLEAENAALAVAKIFALKDEGVREKVVQFQENKRREIYKADEELA",
    "response#3": "MKAVIIMGSKSDLDYSKKIASKLADFGIDAVMRIASAHKTPEKVLEIIKEYEKEDVVFVTVAGRSNALSGFVDANTSKPVIASPPYSDKFGGADIFSSIRMPSGVAPMLVLEAENAALAVAKIFALKDEGVREKVVQFQENKRREIYKADEELA"
  },
  {
    "reference": "MEMMPQYDLSRLPENTALKQQTLPTQQLNLSASVVLSIFFITGGFCLSIGIILLLSAKSTKKIEINYTKTCANCAQLRENSSNFDKACNCSLPFYLPEKMEGDVYMYYKLYGFYQNLYQYILSRSNSQLVGKDIWDTTNCDPFQVSHNDTPIIPCGAIANSIFNDTITLSYNLNSSTQIEVPMLKSGLTWWTDKYVKFRNPRSSNFTSTFAGSSKPLHWAKPIYELDLDDPGNNGFLNEDFIVWMRTAAFPTFKKLYRRLKRVHAFAEGLPAGNYSLSISYNFPVTMFQGEKSIVLSTLTWIGGGGLFLGLTYTVTGALTLLASFAILTIHLMLKRSKLNFL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "MN",
    "response#2": "MGHPG",
    "response#3": "MMSLWDVI"
  },
  {
    "reference": "MAETDAAAAQQPPQIQMRVLGQFIRDMSFENVMAQRGASGDVQPDVNVQVNLDAKKRSTDNQYEIAIKLNITSKAKGMDDVLFVFEIDYCGIFHVEGVPNEQLHPFLLIECPRMLFPFLRRIVSDITRDGGYPPLNLDNIDFMALYRSELARQQAAAQAEQKADA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should be optimized for stability and solubility when expressed in cytoplasm where it will be involved in protein folding, protein transport, protein tetramerization.2. The protein must exhibit the following characteristics: It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state; It also specifically binds to its receptor SecA.. The designed protein sequence is ",
    "response#1": "MQHTDLYLKLNQVAPMLAEQEGEGDNVIKPGDYTLDLKARQVLLTEQGHENAEQILTRMGLLAEGTSLYDPGNILLVHHLYAALRAHSLYHKDQHYVVQNNEVVIVDEFTGRLMAGRRWSDGLHQAVR",
    "response#2": "HTDLYLKLNQVAPMLAEQEGEGDNVIKPGDYTLDLKARQVLLTEQGHENAEQILTRMGLLAEGTSLYDPGNILLVHHLYAALRAHSLYHKDQHYVVQNNEVVIVDEFTGRLMAGRRWSDGLHQAVL",
    "response#3": "HTDLYLKLNQVAPMLAEQEGEGDNVIKPGDYTLDLKARQVLLTEQGHENAEQILTRMGLLAEGTSLYDPGNILLVHHLYAALRAHSLYHKDQHYVVQNNEVVIVDEFTGRLMAGRRWSDGLHQAVV"
  },
  {
    "reference": "MSLYRKRLKIVRGEGIYVWDSEGKRYLDLIAGIGVAILGHNHPKWVEEVGNQLNKLVVAGPMFEHEEKEEMLEELSRWVNFEYVYMGNSGTEAVEAALKFARLYTGRKEIIAMTNAFHGRTMGALSATWKSKYKKDFEPLVPGFKHIPFNDVEAAKEAITRTTAAVIFEPIQGESGIIPAKEEFVKTLRDLTEDVGALLIADEVQSGLRTGKFLAVEHYKVEPDIVTLGKGIGNGVPVSLTLTNFDVERGKHGSTFGGNPLACKAVAVTLRILRKERLVEKASEKFIKVKGKDVVTTRGRGLMIGIVLKKPVGRYVEELQNEGYLVHTSGQRVIRLLPPLIISKEKMMEVKSAIEGVINGDTEG",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein should have pyridoxal phosphate binding to facilitate lysine biosynthetic process via aminoadipic acid, arginine biosynthetic process via ornithine.2. The protein must exhibit the following characteristics: Involved in both the arginine and lysine biosynthetic pathways..3. The protein requires pyridoxal 5'-phosphate to activate a critical catalytic site.4. The protein should be able to bind substrate, pyridoxal 5'-phosphate ligand in a variety of conditions5. The protein should have a fast turnover rate for 2-oxoglutarate + [amino-group carrier protein]-C-terminal-   gamma-(L-ornithyl)-L-glutamate = [amino-group carrier protein]-C-   terminal-gamma-(L-glutamyl-5-semialdehyde)-L-glutamate + L-glutamate.6. The protein should be optimized for activity in L-arginine biosynthesis, Amino-acid biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MCTASVVGGTGFTGGELLRLLAGHPEFEVVQATSRSKENKTVGHAHPNLRDLDLRFSSPEDLESVDALFAATPHGVSMEQIDEFRDAAETVVDLSADFRLDTEDQYDEWYDGHSRPELLDESVYALPELTRDELPGLAAEADAELLVRAGVTLYRVAAVKRGVRRVFVAVDGGMSDNPRPALYGSRYAVRLVRRGGRRAPVTVVGRHCEAGDVLAEDVPLPEDVRAGDLLAVPVTTASIVGGSGFTGGELLRLLAGHPHVEVAEVTSRSKAGKSVGSVHPPLRGSDLRFTEPDDMENVDVLFAATPHGVSMGRIDDFFEIADTVVDLSADFRLGTEAQYDEWYDGHDAPEYMEKAEYALPEINRENLEGLEVPHFMRAGVTLYRVAAVKRGVRRVFVAVDGGMSDNPRPALYGSRYAVRLVRRGGRRAPVTVVGRHCEAGDVLAEDVPLPEDVRAGDLLAVPVTRPRA",
    "response#2": "MASVVGGTGFTGGELLRLLAGHPEFEVVQATSRSKENKTVGHAHPNLRDLDLRFSSPEDLESVDALFAATPHGVSMEQIDEFRDAAETVVDLSADFRLDTEDQYDEWYDGHSRPELLDESVYALPELTRDESDVLIEEARGTVRARARAFREALPEAEVVFAGKALPCREVYRWVADEGLSLDVCSAGELAIARSVGFPAERVLLHGNVKTPEDLKAALGYGVGRVVVDSFDEIEQLGALAEGPQDVLVRVTPGVDPRTHRAVATGVEDQKFGFSLAGGDALEAVLRVVEQPSLRLVGLHCHVGSQVRHVAVYEEAARRVVGLIASCGVRIEQLDLGGGFAVPYLPGEGEFDLGGFAHRVRVALSHECALRRVPVPRLSIEPGRAVVRAGVTLYRVAAVKRGVRRVFVAVDGGMSDNPRPALYGSRYAVRLVRRGGRRAPVTVVGRHCEAGDVLAEDVPLPEDVRAGDLLAVPVTHLRYNNTASVVGGTGFTGGELLRLLAGHPEFEVVQATSRSKENKTVGHAHPNLRDLDLRFSSPEDLESVDALFAATPHGVSMEQIDEFRDAAETVVDLSADFRLDTEDQYDEWYDGHSRPELLDESVYALPELTRDELPGRAGVTLYRVAAVKRGVRRVFVAVDGGMSDNPRPALYGSRYAVRLVRRGGRRAPVTVVGRHCEAGDVLAEDVPLPEDVRAGDLLAVPVTHLRYNNGTVRARARAFREALPEAEVVFAGKALPCREVYRWVADEGLSLDVCSAGELAIARSVGFPAERVLLHGNVKTPEDLKAALGYGVGRVVVDSFDEIEQLGALAEGPQDVLVRVTPGVDPRTHRAVATGVEDQKFGFSLAGGDALEAVLRVVEQPSLRLVGLHCHVGSQVRHVAVYEEAARRVVGLIASCGVRIEQLDLGGGFAVPYLPGEGEFDLGGFAHRVRVALSHECALRRVPVPRLSIEPGRAVV",
    "response#3": "MTASIVGGSGFTGGELLRLLAGHPHVEVAEVTSRSKAGKSVGSVHPPLRGSDLRFTEPDDMENVDVLFAATPHGVSMGRIDDFFEIADTVVDLSADFRLGTEAQYDEWYDGHDAPEYMEKAEYALPEINRENLEDATAKGVKDFIGGNCTVSLMLMAVGXAXRXXGAXRXAXIXERXGGXLIFDEVQSGMGRTGKLFSYMHYDVVPDILTTAKALGGXPXRXSXLLXRLLXNX"
  },
  {
    "reference": "MAVILSVDPCYAGLVGLAEGFRTSNPPRIRECIQCLQAILQFNPPPATQAKTHLQIGRILQQYTKNDDLARRTLEKAVSAELGHGYEEISFEASSILSLVYKDQGQYPLAKQVLRQALEITSGENLYFWQFRLFFQLAEVHACDNEFTASVEVLEMGERIAEQCGSQYMRYNIRHSHNCIIIVLLIMKDVNRVSNILMSTGSFLERWQGQSYQRESLVVFHLLLRVWQLLSVGQAKTVRPYLKQLQQSIQNLTTMTFEGMQPGPNVYEAEKFEWLPREHLCVLVYLVTVMHSMYAGYMDKVLKYSAKALNQVERLKVTSPSALVSVFQLMLLEHTIMCRVVQGQPAHAIKEISQVYQTLKHENCLVRAHKPILHTLLGLYAMSMNLMEQATTHFNIAFKTADNPVLANFVGLNLSIIYIRAGESKQIELSSVMSSIHPSNMATNSHSLQAGYYYVCALRAFFQTRIQDAKKYLRESLKIANAEDLNRLTACSLVLLGHTFLASGNPQEALNMVLPATQLSGKIPDNYIQLWAAGLLRDLYGMLGQPVQASESFHKHHSITKQLIENHMQARKLPEHGLTEWTGAVSPTKSSTIPPQQSFQTWSQPGPSRLS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should emphasize Polar residues compositional bias.2. The designed protein must possess double-stranded DNA binding to support cell division under certain conditions. The designed protein sequence is ",
    "response#1": "LSGHLPTHLKGLDDTLCGGIPFGVLTELVGPPGIGKSQFCMKLALSASFPVAYGGLDGRVIYIDVESKFSSRRVIEMGLESFPEVFHLKGMAQEMAGRILVLRPTSLANFTESIQELKNSILQNQVKLLVIDSMTALLSGENKPGAQRQPQLGWHISFLKSLAEFSRIPIVVTNQC",
    "response#2": "LSGHLPTHLKGLDDTLCGGIPFGVLTELVGPPGIGKSQFCMKLALSASFPVAYGGLDGRVIYIDVESKFSSRRVIEMGLESFPEVFHLKGMAQEMAGRILVLRPTSLANFTESIQELKNSILQNQVKLLVIDSMTALLSGENKPGAQRQPQLGWHISFLKSLAEFSRIPIVVTNQC",
    "response#3": "THLKGLDDTLCGGIPFGVLTELVGPPGIGKSQFCMKLALSASFPVAYGGLDGRVIYIDVESKFSSRRVIEMGLESFPEVFHLKGMAQEMAGRILVLRPTSLANFTESIQELKNSILQNQVKLLVIDSMTALLSGENKPGAQRQPQLGWHISFLKSLAEFSRIPIVVTNQVRSQNRDETSQYSFQAKVKDEFKDNTKTYDSHLVAALGINWAHAVTIRLVLEAKSGQRIIKVAKSPMSPPLAFPFHITSAGIL"
  },
  {
    "reference": "MIGIDIVSIGRISRLKERHGELFLRRFLSEDELALAKSDASIAGLWAAKEAASKALGVGIGAECSFFDIIIEKSPKNAPILKFSPKIYENFNIKEATLSISHDAGFAIAVAIVS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be optimized for holo-[acyl-carrier-protein] synthase activity activity in order to improve fatty acid biosynthetic process.2. The protein must bind to Mg(2+) in order to perform its enzymatic function.3. For general function, the protein need meet that Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein.4. The designed protein should have a high specificity for Mg(2+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MIVGLGTDIVEIARIESRIPAAGDEALLTCRLAKRVLTETEFALFVGSTQPARYLAKRFAAKEAAAKALGTGIGRGVSFQHIEISNNDNGAPQVVFTDGASERLSQLGGVKAHLSIADEQHYATATVILEAK",
    "response#2": "MIVGLGTDIVEIARIESRIPAAGDEALLTCRLAKRVLTETEFALFVGSTQPARYLAKRFAAKEAAAKALGTGIGRGVSFQHIEISNNDNGAPQVVFTDGASERLSQLGGVKAHLSIADEQHYATATVILES",
    "response#3": "MEIGVDIVEIARIRAVYERSGKAFMNKVLTAAEIEQCLAKPDPAVSLAGRFAAKEAISKALGSGIGHKLGWHSIEVLNNEAGKPEVTLHAPHLSYRVSISISHDRHSAVAMALK"
  },
  {
    "reference": "MEEWRIQDRMSQLADVLEAPEEHGVGSVNGLVDMLQLDLGVMVRLDADARPLLSAMKKVLTAGEMSGLDYSGLLSLLDTVVKLAPFETVLEVFSVEDIITALESDDQLVRTSCKVLAVSHPKDLFSTTRIMDVLLQLYFSQRTVPVNAVEDVFQSLCTDGLIRRRILENNYPLLREMRSTHDAILFSRYLVLMTILFRHIDRSEFQKELFVPTTEEVLDSLKKDIFLFINVAKYYRALLEYATDPGAAGGPRDWALSYILPVLHAFGHIYENKEKYIEVHSYARAYLFQLLRTVSYLEDMEVFRELDVKYLHISGDNPDVMHFMAIFNPMYLYRHHFDLLTSQVTVKPSFLPVLKNIVSCQETFEILKPALTASAILIMPYSEQMVLLGQLSSYPHSTEYLVQELPKVMSNLICNENGPISEPETIELRKKVFENLLLYSATVLNVWYEPLLDEYTNICSGKTSFVRTEVVDMYL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be optimized for stability and expression in nucleus where it will participate in proteasome regulatory particle assembly.2. For general function, the protein need meet that Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the 19S regulatory complex (RC) .; Involved in DNA mismatch repair in slow-growing cells The designed protein sequence is ",
    "response#1": "LEKLLIFEKQVRQASDTSTNTKVLIYIADLLFRAGDFQGLNEQLVSLFKKHGQLKQSMTSLVQHVMTYLPGIDDLKTKINLIETLRTITDGKIYVEVERARLTQLLSQIKEEQGDIKSAQEILCNEPVETYGSFDLKEKVAFILDQVRLFLLRSDYYMASTYTKKINVKFFEKEDVQSLKLKYYEQKIRIGLHDDAYLDVCKYYRAVYDTAVVQEC",
    "response#2": "LEKLLIFEKQVRQASDTSTNTKVLIYIADLLFRAGDFQGLNEQLVSLFKKHGQLKQSMTSLVQHVMTYLPGIDDLKTKINLIETLRTITDGKIYVEVERARLTQLLSQIKEEQGDIKSAQEILCNEPVETYGSFDLKEKVAFILDQVRLFLLRSDYYMASTYTKKINVKFFEKEDVQSLKLKYYEQKIRIGLHDDAYLDVCKYYRAVYDTAVVQEC",
    "response#3": "HNLIKVYEYNEPDIKLLSNVNPDLIVHSSNVIYEDVLSNLSLLNNKQYFPILLNFIGSTDVFPRLEQDYFTTKLKHLPADKLYVILLEFSKFSHSRKFLLSNATLTNDYLLDNDNLTFVNNELWHTKLQVLENLVNATDSDSPELAHWKPYLQDSFNLMRHGKKIC"
  },
  {
    "reference": "MNISELIFKLGGTGSVSKLLNTNPSTVSNWKKNNKIPKSYMNELDILLKERNLITFTENEDDKSITPIRKNSKFSILVIISGGVSCYKILELIRLYYKNDFDIEVVLTKSAQKFITPLIVTSLINKKCFTELFSSEENENMNHINLARNKNLILIAPCTANFIAKMTHGIADDLALNIILASNSKIFIAPSMNPYMWENKATQDNLETLKKRGVVLINPNSGLMACGENGTGRLPEINEIFSFTTNYLLKKDVKKCIGFQSQLKILITAGPTQEKIDPIRYVSNNSSGKQGYALAEEFLKLGAHVTLISGPTNINKPNVNKFIEVLSAEEMLSQVENNIDCDIFISSAAVCDWKLIPYTLKNKKLSNNDKLKKNKDIFFKLETNPDIIKSVSKNKKRPRLVIGFAAETKNLIQNAKKKLKEKQLDMIIANLIEKENAIFGEEFNKIFIIDKQCNIQESEKLTKKEIANLIISKTLKLHEETYAKNYNRKSISI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. We require a protein that can specifically bind to the metabolites produced in the coenzyme A biosynthesis, CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis pathway.2. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.3. The designed protein must have at least one DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domain.4. The protein design must be able to perform FMN binding, metal ion binding, phosphopantothenate--cysteine ligase activity, DNA binding, phosphopantothenoylcysteine decarboxylase activity in order to contribute to coenzyme A biosynthetic process.5. The protein should have a high affinity for CTP.6. The protein with the reaction H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2, the Proton donor must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MKMLFIISASIAIKKCNVILKALSNQQIFINCIVTNNAKKMTNLKDLNKSIKGKIYSDSSEKNNKMFHIKLTRKTDLIVVCPATANIIAKYANGFADDLASTSLIASNKQIIFIPAMNAEMWNNSINQKNVSKLMDMGVEFIGPEYGHLSCDEVGLGRLSNAKKIQAKIAIEDFFQKNKKCLITAGPTLEPIDPIRYISNXSSGKQGYEVAKQMILSGAKVTLVSGPTNIQAPYQAKLIKVKTAKEMFTAAKKHSGVDIAIFTAAVSDASPRKLFNYKIKKENLKNIILKKNRDILQNISSLKKNRPNIVIGFAAETTNHINNAKKKLQSKKCDAIIVNKIDNNKVFGSDHNKVSFITKDKVEFWDRMAKEKVAKELADKIVSLFK",
    "response#2": "MKMLFIISASIAIKKCNVILKALSNQQIFINCIVTNNAKKMTNLKDLNKSIKGKIYSDSSEKNNKMFHIKLTRKTDLIVVCPATANIIAKYANGFADDLASTSLIASNKQIIFIPAMNAEMWNNSINQKNVSKLMDMGVEFIGPEYGHLSCDEVGLGRLSNAKKIQNSMEYFFSPSSLKGKRIVITAGPTREALDPARYLSNRSSGKMGYALARTAARRGAEVTLVSGPVDIKAPSWVATIPVKTARQMHARVLELSEDADIIVKAAAVSDFKPKNYCRQKIKKNQGLTSIEVEENPDILSDLGESRRRGSVLVGFAAESRNHEAEGRRKLLQKKTDLIVVNDILSSKTGFDVDTNQVILIEKNGDLIDLDLMSKRDVAERIVNKVKQLL",
    "response#3": "MKMLFIISASIAIKKCNVILKALSNQQIFINCIVTNNAKKMTNLKDLNKSIKGKIYSDSSEKNNKMFHIKLTRKTDLIVVCPATANIIAKYANGFADDLASTSLIASNKQIIFIPAMNAEMWNNSINQKNVSKLMDMGVEFIGPEYGHLSCDEVGLGRLSNAKKIQDALDFFSANLLKGKRILVTSGPTHEPIDPVRYIANRSSGAQGAALGRALAALGAEVVFVTGPADVPPPEGVEVERVETAREMLDAVQAALPADAAVFAAAVADWRVSSTSDRKLKKSSDGLPQLTFAENPDILRTVSEMTQGRPGLVVGFAAETNDVVDNATAKRARKGCDWIVANDVSPATGIMGGTENAVVLIDTHKNIHSISQHSKITLAKQLIAHIASQLS"
  },
  {
    "reference": "MKNINPSQTAAWQALQHHFDAMKEVQISELFAQDSDRFAHFSATFDDLMLVDYSKNRITTETMEKLHALARETDLSAAIQSMFAGEKINRTEDRAVLHVALRNRSNTPILVDGKDVMPEVNAVLAKMKDFSERVIGGEWKGYTGKTITDVVNIGIGGSDLGPFMVTEALKPYKNHLNMHFVSNVDGTHIAETLKPLNPETTLFLVASKTFTTQETMTNAHSARDWFLNTAKDEKHVAKHFAALSTNAKAVGEFGIDTNNMFEFWDWVGGRYSLWSAIGLSIILSLGFENFEKLLSGAHAMDKHFASTPAEKNLPVLLALIGIWYNNFFGAETEAILPYDQYMHRFAAYFQQGNMESNGKSADRNGNPVDYQTGPIIWGEPGTNGQHAFYQLIHQGTKLIPCDFIAPAVSHNPLSDHHSKLLSNFFAQTEALAFGKSRDVVEAEFAAAGKSAKDVEHVAPFKVFEGNRPTNSILLREITPYSLGALIALYEHKIFTQGAILNIFTFDQWGVELGKQLANRILPELENDSTIDSHDSSTNGLINRFKAWRN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein with carbohydrate derivative binding, glucose-6-phosphate isomerase activity is needed to enhance gluconeogenesis, glycolytic process in a specific context.2. A key design requirement for the protein is the optimization of the active site, Proton donor to achieve high catalytic efficiency for alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate.3. The protein must exhibit the following characteristics: Catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate..4. A protein that can modulate the overall flux of metabolites through the Carbohydrate degradation, D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 2/4, glycolysis pathway, enhancing or suppressing its activity as needed. The designed protein sequence is ",
    "response#1": "VPYSHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGTRLIPCDFIVPMTAQGREDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERERLAPHKEFPGNRPSNSILIDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGKQLAKTLMWLGEETRAILPYSNDLGRFPAYLQQLAMESNGKSVRADGSPIGTTTGAVFWGEPGSNGQHAFYQLLHQGTRLVPADFIGFAEPTHDLPAADGAGSMHNILMSNLFAQSRVLAFGKTPEELTREDTAPDLIAHKTMPGNQPSTTILAPRLTPSVLGQLIALYEHQVFVEGIIYGIGSFDQWGVELGKTQAVRILAERKDMVVLPYKDSLALLSRYLQQLVMESLGKEKDLDGNTVYQGIAVYGNKGSTDQHAYVQQLREGVPNFFVTFIEVLEDRQGSSIELEPGVTSGDYLAGFIQGTRQALYENHRDSITITIPEVNPRTVGALVALYERAVSFYGSLVNVNAYHQPGVEAGKKAAASALAWLGE",
    "response#2": "LKQIEEFVAKVLKGTIKPPTADKFTDIISVGIGGSALGPQFVSEALAGDFPPMGIHFIDNTDPAGIDRVVTRLKDRLKSTLVIVTSKSGGTPETRNGMLEMRHAYEKNGLDFPKYAVAVTMPGSKMDQVAHDWLARFPMQDWVGGRTSELSAVGLLPAAGVVWRINSFDQWGVELGLAAERLCAEETRAILPYSNDLGRFPAYLQQLAMESNGKSVRADGSPIGTTTGAVFWGEPGSNGQHAFYQLLHQGTRLVPADFIGFAEPTHDLPAADGAGSMHNILMSNLFAQSRVLAFGKTPEELTREDTAPDLIAHKTMPGNQPSTTILAPRLTPSVLGQLIALYEHQVFVEGIIYGIGSFDQWGVELGKTQAVLRGQAGMVVLPYRDRLQVFSKYLQQLVMESLGKKFNRKGEVVNQGISVFGNKGSTDQHAYVQQLRDGIDNFFCIFIELLDSPSTSIFDKKENPKEYLSGFLQGTRSALSSENRQSITITLEKLNCFSLGALIALFERAVSFYAELVNINAYDQPGVEAGKKAAANCLAELGKIAAEIGNAVLVKMKQFCDRVIGGEWKGYTGRIITDIVNIGIGGSDLGPYMVTEALRPYKNHLNMHYVSNVDGTHIAETIKDLDPATTLFLVASKTFTTQETMTNAHSARDWFLKTAGDEQHVARHFAALSTNAKAVAEFGIDTENMFEFWDWVGGRYSLWSAIGLSIAAGTWDNIGRMAAGLEVPYSHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGTRLIPCDFIVPMTAQGREDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERERLAPHKEFPGNRPSNSILIDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGKQLAKT",
    "response#3": "QSHVILTYDHYLRGLPAYFQQLDMESNGKSVTLDGTDVDYSTGPVIWGGEGTNGQHAYHQLLHQGTALIPADFIMPLQSHNPLGEHHDQLASNCFGQTQALMQGRTYDEALAELSNSKLDEQQKPLIAKHKVMVGNKPSNTLLMDKLTPTTLGALIALYEHRTFVQGAIWQINSFDQWGVELGKQLGNDINARIEVVSTEVLVTYEPALKATAEWWKQLFGESEGKDGKGIFPAAAAFTTDLHSLGQYIQEGMRNLFETVVHVERPSVDIPLPPGDEGDGLGFLGGQTLHHVNHTAFLATRLAHTEGGVPNLTLTLPELTPYHYGYMLYFFMKACAISGYLLGVNPFDQPGVEAYKQNMYALSSLEGIDKESIDLKQIEEFVAKVLKGTIKPPTADKFTDIISVGIGGSALGPQFVSEALAGDFPPMGIHFIDNTDPAGIDRVVTRLKDRLKSTLVIVTSKSGGTPETRNGMLEMRHAYEKNGLDFPKYAVAVTMPGSKMDQVAHDWLARFPMQDWVGGRTSELSAVGLLPAAGDDVGGRYSVLTAVNDMEDALAMETRAILPYSNDLGRFPAYLQQLAMESNGKSVRADGSPIGTTTGAVFWGEPGSNGQHAFYQLLHQGTRLVPADFIGFAEPTHDLPAADGAGSMHNILMSNLFAQSRVLAFGKTPEELTREDTAPDLIAHKTMPGNQPSTTILAPRLTPSVLGQLIALYEHQVFVEGIIYGIGSFDQWGVELGKTQAAKLSGQVLFG"
  },
  {
    "reference": "MGPLSAPPCTQHITWKGLLLTASLLNFWNLPITAQVTIEALPPKVSEGKDVLLLVHNLPQNLAGYIWYKGQLMDLYHYITSYVVDGQINIYGPAYTGRETVYSNASLLIQNVTREDAGSYTLHIIKRGDRTRGVTGYFTFNLYLKLPKPYITINNSKPRENKDVLAFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPSVTRNETGPYECEIRDRDGGMRSDPVTLNVLYGPDLPSIYPSFTYYRSGENLYLSCFAESNPPAEYFWTINGKFQQSGQKLSIPQITTKHRGLYTCSVRNSATGKESSKSMTVEVSAPSGIGRLPLLNPI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Ensure the presence of a signal peptide within the protein.2. The protein design should be able to enhance the efficiency of heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules. The designed protein sequence is ",
    "response#1": "TPEPRSFEICSRQPEKQILSIVDKDLPPHTYPFKAALEHGSSNNWTVEIRGQDELAMGLKKELEPGEYNIFVKLTDSQGKAQVTQVKAQVCECEGTAKNCERRSYIVGGLGVPAQ",
    "response#2": "TPEPRSFEICSRQPEKQILSIVDKDLPPHTYPFKAALEHGSSNNWTVEIRGQDELAMGLKKELEPGEYNIFVKLTDSQGKAQVTQVKAQVCECEGTAKNCERRSYIVGGLGVPAQ",
    "response#3": "TPEPRSFEICSRQPEKQILSIVDKDLPPHTYPFKAALEHGSSNNWTVEIRGQDELAMGLKKELEPGEYNIFVKLTDSQGKAQVTQVKAQVCECEGTAKNCERRSYIVGGLGVPAL"
  },
  {
    "reference": "MSSVNSIPTATSTVYISVLPATATPSGGSGGNVLHEDLNKFYDYGNTSWILACTPLCLIMVPGVAFFYSGLARRKNTLALIMLSMLGLCVSFFQWYFWGYSLAFSQTGTSGYIGNLRHFAFIRTLADYSPGSNNIPELVFANFQGMFAAITVALFTGAAAERGRIGPMLIITFVWLTVVYCPIACWIWNPNGWAFKFGVYDFAGGGPVEVGSGFAALAYTVCLGRRSKFVEEQFRPHSVLNVVLGTSLLWFGWLGFNGGSAYGSNLRAAMAITNTNLAGAVAGLVWVIYDYIFRTRKWSTIGFCSGVVAGLVAATPCAGFVSPHASLAIGAITGLCCNWAIKLKSHMRIDDAMDIFAIHGVAGFVGTFLNGLFAVDYIAAMDGIYVGENKIRGGWFDHHWRQLGLQMAYICAVGAYDFVVTFIILFITDKIPYLQLRVSPDAEEIGVDADQIGEYAFDYIEERREYKHWKISPAGVPEEIIISNGVAQPTGNVAAPGKILESTNPLELGLTI",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein with methylammonium transmembrane transporter activity is needed to enhance methylammonium transmembrane transport in a specific context.2. The protein must exhibit the following characteristics: Transporter for ammonium to use as a nitrogen source..3. The designed protein should contain Cytoplasmic, Extracellular topological domains. The designed protein sequence is ",
    "response#1": "YLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGALFYYLFGFAFAFGAPSNGFIGKHFFGLKQVPQVGFDYSFFLFQWAFAIAAAGITSGSIAERTQFVAYLIYSAFLTGFVYPVVSHWIWSADGWASASRTSGSLLFGSGVIDFAGSGVVHMVGGVAGLWGALIEGPRIGRFDHAGRSVALRGHSASLVVLGSFLLWFGWYGFNPGSFLTILKSYGPPGSIHGQWSAVGRTAVTTTLAGSTAALTTLFGKRLQTGHWNVIDVCNGLLGGFAAITAGCSVVDPWAAIICGFVSAWVLIGLNALAARLKFDDPLEAAQLHGGCGAWGVIFTALFARKEYVDQIFGQPGRPYGLFMGGGGRLLGAHIVVILVIAAWVSFTMAPLFLVLNKLGLLRISAEDEMAGMDQTRHGGFAV",
    "response#2": "LLFSAYLVFVMQLGFAMLCAGSVRAKNAMNIMLTNVVDAVVGSLSYYLFGFAFAFGDSNPFIGASYFALKDIPSSSYDYSFFLYQWAFAIAVAGITSGSIAERTQFTAYLVFSFFLTGFVYPVVAHWLWSSNGWLSPNSTYLLFGSGAIDFAGSGVVHLVGGIAGFWGSIVEGPRVGRFDAFGNPVKMRGHNATLVVLGTLLLWFGWFGFNPGSFNKILVAYPHMADQGNWTSVGRTAVTTTLAGSTAGIVTLFGRRLLVGHWDAMDVCNGVLGGFVAITSGCSVVEPWAAILCGFCAAWVLIGLNILALKFKFDDPLEAAQLHGGCGAWGLIFTGLFAKEEFVLQAYNSGKTQIIRPSGLILGGGWGLFGAQIVELLSIVVWVSLTMGPLFYLLQKLGILRISSDEEVAGLDISSHGGYAY",
    "response#3": "NDFYYRYPSFQDVHVMIFVGFGFLMTFLKRYSFGGVGFNFLIAAFGIQWALLMQGWFHFLDHSTGKIYIGVENLINADFCVAGSLIAYGALLGKVSPVQLLVLTLFGVTLFAVEEYIILHLLHCRDAGGSMVIHAFGGYYGLGISWVLYRPNLHQSKRLHGSVYHSDVFAMIGTLFLWMFWPSFNSAITDHGDGQHRAAINTYLGLASCVLTTVALSSMHDKRGRLDMVHIQNATLAGGVAMGTAAEFMISPYGALIVGFCCGIISTFGYLYVTPFLEKYLKLQDTCGVHNLHAVPGMLGGFVGAIVAAAATESVYSKEGLINTFNFEGKYADRSVGTQGGYQAAGTCVAVAFGLVGGAIVGFILKFPIWGDPADDNCFDDEAL"
  },
  {
    "reference": "MAKKTKAEDPKKRIITLRKEIGRHNDLYYKENAPVITDKEFDILVKELQKLESENPDLADVSSPTAQVGSDLSPQFSKFKHKVPVLSLENTYNETELSEWLEKTGIEENYSLEWKIDGASILLYYEKGKLTNCVTRGSGGIGDVVTENVKTISTIPHTLSEEMNLTVRGEIFMTFADFEEFNEEYGGKFANPRNLAAGSIKQKDPLDVAKRPLRIYVYDVYFSSSRKGINTHKDILSLLKKEKFPLAPDTTILTGKKLLREIESFRKKKDKMPFPVDGLVIKLDSLNLRESLGETSHSPRWARAFKFDALLKESTIEEIDFAIGRTGKVTPRAKVTPISLAGTTVTYATLHNQDYINQLGAGIGAKVLISKRGEIIPAVEKVTFPPKTIFVLPNQCPSCNTKLTKVDDSVDFFCTNRHCPERKLNQLIFFCSKKQMNIEGLGERQIQIFFEKGWVKDIPDLYTLEKYKPTLLELDGFGEKSVKIIFDAIEKSKEKDFRFTLPSIGLNEVGPKVTEILIENGYDSWDKLLTLSKSKTANEDLKAIHGIGPRTIEALLTHLKDKETLKLVATLKKLGLKFQADETEKSDLQPFVGQSWCVTGSFENFQPRDLAMDLITKHGGKKVTSVSSKTTHLLYGPGAGSKLDKATELGVKLVTESEFLDLLKQEGIAID",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein must have a N6-AMP-lysine intermediate active site that is highly conserved among related enzymes.2. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency.3. The designed protein should be optimized for DNA binding, DNA ligase (NAD+) activity activity in order to improve DNA repair.4. The protein contains novel BRCT domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "SGFTPVVHDKPMLSLNNGFADEDVIAFDKRVADGLDKATDLAGTVTEPVEYACELKFDGLAISLRYENGRFVQASTRGDGTTGEDVTENIRTIRAIPLTLKGKRIPRMLDVRGEVLMFKRDFARLNERQRAAGQREFANPRNAAAGSLRQLDSKITASRPLSFFAYGIGVLDGADMPDTHSGLLDWYETLGLPVNRERAVVRGAAGLLAFFHSVGERRESLPYDIDGVVYKVNRRDEQDRLGFVSRAPRFALAHKFPAXXX",
    "response#2": "AMGAGVEVTPYFFDVLHLDGADLLDVSAAERAAVLERLVPAEHRVPRVVTADLGVAEEFLAGALRSGHEGVVVKSLAAPYEAGRRGASWVKVKPVHTLDLVVLAVEWGSGRRQGWLSNIHLGARK",
    "response#3": "AMGAGVEVTPYFFDVLHLDGADLLDVSAAERAAVLERLVPAEHRVPRVVTADLGVAEEFLAGALRSGHEGVVVKSLAAPYEAGRRGASWVKVKPVHTLDLVVLAVEWGSGRRQGWLSNIHLGARR"
  },
  {
    "reference": "MSKHHPDLVLCRRQPGITVGKLCERCDEKCPICDSHVRPTTLVRICDECAFGSSQDRCIICGAPGVSDCYYCSECTRMEYDRDGCPRVINLGSSRTDWFYERKKFKNAGKEMPGATY",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be expressed specifically in the cytosol, nucleus, precatalytic spliceosome The designed protein sequence is ",
    "response#1": "KKSINGLINKVNVSNIKYIVPELFGENLVRGRGLFCRSIMKAQAASLPFTPIYAAMAAIVNTKLPQVGELLLSRLIIQFRKAFKRNDKAVCISSTTFIAHLCNQQVVHEMLAAQILLLLLHKPTDDSVEIAVGLTREVGQHLEEMSGPIALAVFDQFRNILHEADIDKRVQYMIEVLFQVRKDC",
    "response#2": "KSINGLINKVNVSNIKYIVPELFGENLVRGRGLFCRSIMKAQAASLPFTPIYAAMAAIVNTKLPQVGELLLSRLIIQFRKAFKRNDKAVCISSTTFIAHLCNQQVVHEMLAAQILLLLLHKPTDDSVEIAVGLTREVGQHLEEMSGPIALAVFDQFRNILHEADIDKRVQYMIEVLFQVRKR",
    "response#3": "KKSINGLINKVNVSNIKYIVPELFGENLVRGRGLFCRSIMKAQAASLPFTPIYAAMAAIVNTKLPQVGELLLSRLIIQFRKAFKRNDKAVCISSTTFIAHLCNQQVVHEMLAAQILLLLLHKPTDDSVEIAVGLTREVGQHLEEMSGPIALAVFDQFRNILHEADIDKRVQYMIEVLFQVRKDS"
  },
  {
    "reference": "MQATELALLSTGQMQQADAMTIAAGRPGIVLMERAGAAVAAAALARSAEGAAITVVCGPGNNGGDGFVAARILRERGRQVRVLLHGRLEALTGDAAAASARWDGPILPLARIREAPGYLFIDALFGAGLTRALAGEAAAAVAFMNETGRPIIAVDVPSGVSGDTGQVDGIAVKASETVTFFRLKPGHLLLPGRSLCGHVSLSDIGILPEAAAGAATFRNDVGLWRDVWPRHGLDTHKFRRGAVAVVAGGVAGVGAPRLSARAALRIGAGLATILCEPAALMAHAARGPDALMQRSAADAAALTAFLDDPRVSAVLAGPALGLGERGAELVGAIAGCATPAVLDADALTLLARAPGGAAALLAGRRAACVLTPHEGEFQRLFGGEAGIAKERSKLERARKAARRCGAIVVYKGADTVVAAPDGRAAINTTGGPALATAGSGDVLAGLICGLLAQGMPAFEASCAAAWLHGRAGERLGIGLIADDLPEAIPALMRETLPV",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.2. The desired protein should be able to perform (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate3. The protein must have high specificity towards NADPHX epimerase activity, kinase activity, ATP binding, NADHX epimerase activity.4. The designed protein should have a high specificity for (6S)-NADPHX, K(+) binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MRDIENRIFAGGMPVAALMEKVAGLIADRIQTKLAEDTTYFPHSPSFRIGILVGPGHNGGDALVVARELYFRGYEVGIYCPFSKRKELTSQHLQYAQSIGIICYESIELLADCHLFIDGLFGFGLERAISDPIATAINQLNEWHKPVIAIDLPSGLHTDTGEVLGTAVRATHTLCLGLWKLGLLQDQALDCVGKAELIDFDIPLADIVETGIPAPIPSRPADTHKGNYGKVLFIAGAQNYLGAPQFSALSFLKTGGGLSYLATPKGVAPHLGSRSRELVLYPLWETDTGSVSMKNLEGLRGLCTQMDMVVMGPGLSLNEETQELVRILTEEIPVPLLLDGDGITAVAKNPKILKRRKAPTILTPHPGEMGRLTGKTVSEIRGDRVGILRETCRQLKAFIVLKGAHSLIGLPDGEIWINLSGNPGMATAGSGDVLTGTIAAMTGRGFPIGDAVKMGVFLHGLAGDLAAKSLGEDGVTAEDIMQKLPEAVRTLLALSRDPQSHKGDHGTVLVIGGGPYSGAPALSAQAAFRAGADLVYVATPESVADAVAGYSENLIVEALPGDRLAPVHVDTLLALAEDADAVLVGPGLGDAEGSLDAVAGFLESYAGQAVVDADPLRIVPEVETDADLVCTPHQGELTAMGGPREDDWRDRADAVESFATEVGATLLVKGAYDVVSDGERTRVNRTGNPGMTVGGTGDVLAGITAALVTALDDPVQAAGIAAYANGRAGDFAVEEHGYGLVATDLVLVIGGSPGLTGAAALAATAALRAGAGLVTLATPARLQELMAGKLTEAMTCPLPDTAEGMVCATAVTAALERGRGASAVVLGPGLGQGAGPREFVRAFSRRCPVPVVVDADALAMVEPEGEREGQLILTPHPGEAARLLQCGVAEIGADRLAAARRLAARYRAVVVLKGARTVVTAPGRGAFIDPAADPALAAGGTGDVLAGAIGALLAQGCPPLEAAVAGVYLHGVAGGLASQELGDAGILAGEVAAAL",
    "response#2": "MRDIENRIFAGGMPVAALMEKVAGLIADRIQTKLAEDTTYFPHSPSFRIGILVGPGHNGGDALVVARELYFRGYEVGIYCPFSKRKELTSQHLQYAQSIGIICYESIELLADCHLFIDGLFGFGLERAISDPIATAINQLNEWHKPVIAIDLPSGLHTDTGEVLGTAVRATHTLCLGLWKLGLLQDQALDCVGKAELIDFDIPLADIDGLPADRWRALAVAGSEGKAGAATLVVLAALRAGAGLVTLAARPEVLRTALPGVPEAMGVPLPGNGPLGPGDLAPLRAALRGKTAFAMGPGIPRGPQTGQLLGELLAALDPGCATVVDADALNALAEERDAIAERLRKAPVRPVLTPHPGEFSRLTGEEVERVESDRIDAAVRASSRFGACVVLKGARTVVADPEGTSAVCAHGNPGMATAGAGDVLTGITAAVLARRSGGGSTGERARLAVFLHALAGDLAARRTGETGLVASDILLALSRDPQSHKGDHGTVLVIGGGPYSGAPALSAQAAFRAGADLVYVATPESVADAVAGYSENLIVEALPGDRLAPVHVDTLLALAEDADAVLVGPGLGDAEGSLDAVAGFLESYAGQAVVDADPLRIVPEVETDADLVCTPHQGELTAMGGPREDDWRDRADAVESFATEVGATLLVKGAYDVVSDGERTRVNRTGNPGMTVGGTGDVLAGITAALVTALDDPVQAAGIAAYANGRAGDFAVEEHGYGLVATDLLLALSRDPQSHKGDHGTVLVIGGGPYSGAPALSAQAAFRAGADLVYVATPESVADAVAGYSENLIVEALPGDRLAPVHVDTLLALAEDADAVLVGPGLGDAEGSLDAVAGFLESYAGQAVVDADPLRIVPEVETDADLVCTPHQGELTAMGGPREDDWRDRADAVESFATEVGATLLVKGAYDVVSDGERTRVNRTGNPGMTVGGTGDVLAGITAALVTALDDPVQAAGIAAYANGRAGDFAVEEHGYGLVATDL",
    "response#3": "MRDIENRIFAGGMPVAALMEKVAGLIADRIQTKLAEDTTYFPHSPSFRIGILVGPGHNGGDALVVARELYFRGYEVGIYCPFSKRKELTSQHLQYAQSIGIICYESIELLADCHLFIDGLFGFGLERAISDPIATAINQLNEWHKPVIAIDLPSGLHTDTGEVLGTAVRATHTLCLGLWKLGLLQDQALDCVGKAELIDFDIPLADIVLVIGGSPGLTGAAALAATAALRAGAGLVTLATPARLQELMAGKLTEAMTCPLPDTAEGMVCATAVTAALERGRGASAVVLGPGLGQGAGPREFVRAFSRRCPVPVVVDADALAMVEPEGEREGQLILTPHPGEAARLLQCGVAEIGADRLAAARRLAARYRAVVVLKGARTVVTAPGRGAFIDPAADPALAAGGTGDVLAGAIGALLAQGCPPLEAAVAGVYLHGVAGGLASQELGDAGILAGEVAAALSHATNAFPLPRRARDAHKGNFGHVLVIGGDVGFAGAPLLAGEAAARVGCGLVTLATRPQHAGMQVAARPELMAWGVDRPAQLDPLLDRASVVAIGPGLGQSPWAAGLLARVLETNLPLVVDADALTLLARDPVQNERWVLTPHPGEAARLLDCSTAEIQGDRFTAVRELQSRYGGAIVLKGCGSLVATRKSGVTVCTDGNPGLASGGTGDVLTGVIAGLMAQGLEPESAALTGACLHGAAADRAVLEGERGMLAGDLMPWLRRLVNA"
  },
  {
    "reference": "MVMAEIGGHLAHQLPLHNHNHNQTGLQPSLVMNHHLDLDCHGHDVIKKQRLSHCVGCGGQIHDQYILRVAPDLEWHAACLKCQECRQFLDESCTCFVRDGKTYCKRDYVRLFGTKCDKCGNSFSKNDFVMRAKTKIFHIECFRCSACARQLLPGDEFALRDAGALYCKEDHDVLEKSSQSSLTSSSVESNNNISSSNNNNTNLSNNNHSSELGSMSDSGSESGSHKSIRDKRPSGPSDGKPTRVRTVLNEKQLHTLRTCYNANPRPDALMKEQLVEMTSLSPRVIRVWFQNKRCKDKKKTIQMKLQMQQEKEGRKLGYGAMQGIPMIASSPVRHDSPLNLQGLDVQTYQPPWKALSDFALHADLDSNGAINTHTPAFQQLVNQMHGYDLNGMPILPPHPHSHPAQGPPHQHPPPPGGPHNHQNQQPNQQPGGSSLDSGITSHHHPDSTDSYVTYLESDDKSKLALTPSSSSSASAGTSISSPPSGVGAGGGGAVGGGSGVLGLGVVANQSATEQLMQMLQKVTGSASPASHAVL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that contributes to neuron fate specification, amnioserosa maintenance, regulation of gene expression, axon guidance by functioning in nucleus with RNA polymerase II-specific DNA-binding transcription factor binding, cis-regulatory region sequence-specific DNA binding, RNA polymerase II cis-regulatory region sequence-specific DNA binding, RNA polymerase II transcription regulatory region sequence-specific DNA binding, DNA-binding transcription factor activity, RNA polymerase II-specific.2. The designed protein should contain at least one Homeobox domain that can specifically recognize and bind to DNA.3. Target a Polar residues, Pro residues, Basic and acidic residues compositional bias in the protein's composition for improved properties.4. The designed protein must have at least one Homeobox, LIM zinc-binding domain. The designed protein sequence is ",
    "response#1": "PDFSGLPSFLPQTPSSRPVTYSYSSNLPPVQPVREVTFREYAIDPTASKWHHPRSNLAHCYSTAEEIMHSRDNSSSVAAEMFGKNAAGVYHHHHHPATPGNTSSNFYSAVGRNGVLPQAFDQFFETAYGTAT",
    "response#2": "PDFSGLPSFLPQTPSSRPVTYSYSSNLPPVQPVREVTFREYAIDPTASKWHHPRSNLAHCYSTAEEIMHSRDNSSSVAAEMFGKNAAGVYHHHHHPATPGNTSSNFYSAVGRNGVLPQAFDQFFETAYGTAD",
    "response#3": "MYPGTLRSPGGSSTAGVGTSGGSGSPLPASNFTAAPVYPHYVGYPHMSNMDPHGPSLGAWSSPYSPPREDWSTYPGPPSTMGTVPMNDMTSPVFGSPDYSTLGPTSGASNGGSLPDAASESLVSLDSGTSGATSPSRSRHSPYAWMRKTVQVTGKK"
  },
  {
    "reference": "MELKPGALAMEILLLELLLCCSLTVIRGSPLLLFANRRDVRLVDVEVGRSESAVVVSDLEDAAAVDFLFSEGLIFWTDVSEEAIKQTYYNLSTNSMKEATLGKGQTVVVSGLDSPDGLACDWLGHKLYWTDSETNRIEVANLDGTSRKVLFWQDLDQPRAIALNPSHRYMYWTDWGEEPRIERAGMDGSNRQIIVQKQIYWPNGLTIDLDEQKLYWADAKLSFIHRANLDGTSREAVVEGSLTHPFALTLYDETLYWTDWQTRSIHACNKHSGEKHREILNGIYSPMDIQVLGQERQPNIQTPCSERNGGCSHLCLLSPIPPFYSCACPTGVKLKEDGKTCRPGAEQVLLLARRTDLRRISLDLPDFTDIVLQVNDIRHAIAIDYDPVEGYVYWTDDELRAIRRARIDGSDAQTLITNEINHPDGIAVDWVARNLYWTDTGTDRIEVTRLNGTSRRVLISENLDEPRAIVLDPINGYMYWTDWGELPKIERANLDGTDRVVLLNTSLGWPNGLAIDYIAGKLYWGDAKTDKIEVINVDGTNRMTILEDKLPHIFGFTLLGEYIYWTDWQRRSIERVHIANSTREVIVEQLPDLMGLKATKVMEAEGTNGCAENNGGCSHLCFHRPQGLSCACPMGLELLSDLRTCVVPEAFLLFTNRADIRSISLGTNSNDVPIPLTGVKEASALDFDVLENRIYWTDVSTKTISRAFMNGSSVEPVIEFGLDYPEGMAVDWMGRNLYWADTGTNRIEVARLDGQYRQVLVCKDLDNP",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should be able to enhance the efficiency of posterior lateral line neuromast hair cell development, anterior lateral line neuromast hair cell development. The designed protein sequence is ",
    "response#1": "PEPAQPAEGDNVTLVVHGLSGELLAYNWYAGPTLSLTFLVASYIVSTGDETPGPAHTGREAVRPDGSLDIHGALPGHTGTYILQTLNRQFQTEVGYGHMQVL",
    "response#2": "PEPAQPAEGDNVTLVVHGLSGELLAYNWYAGPTLSLTFLVASYIVSTGDETPGPAHTGREAVRPDGSLDIHGALPGHTGTYILQTLNRQFQTEVGYGHMQVK",
    "response#3": "PEPAQPAEGDNVTLVVHGLSGELLAYNWYAGPTLSLTFLVASYIVSTGDETPGPAHTGREAVRPDGSLDIHGALPGHTGTYILQTLNRQFQTEVGYGHMQVI"
  },
  {
    "reference": "MKLHELKPAEGSRKVRNRVGRGIGSGNGKTAGKGHKGQNARSGGGVRPGFEGGQNPLYRRLPKRGFTNPTRKEFAVVNLEKLNRFEDGTEVSPELLLETGVVSNAKDGIKILGNGKLEKKLTVKANKFSASAVEAIEAAGGKTEVI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have structural constituent of ribosome and be active in large ribosomal subunit, cytoplasm.2. The protein must exhibit the following characteristics: Binds to the 23S rRNA..3. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "VAEETAEFDDGAYHIDARDIADDADSSDITKVLGDGQVRQELHVIADAFSDTARTLIEDAGGSAA",
    "response#2": "VAEETAEFDDGAYHIDARDIADDADSSDITKVLGDGQVRQELHVIADAFSDTARTLIEDAGGSAF",
    "response#3": "VAEETAEFDDGAYHIDARDIADDADSSDITKVLGDGQVRQELHVIADAFSDTARTLIEDAGGSAV"
  },
  {
    "reference": "MRVLKFGGSSLADADRFSRAADIIANNAQQGPVSVVLSAPGKVTNKLVSVIENSIQFGEADVQLADLEAVFKELLAGLKALEPGFDQEALDAKLASSLGQLKQYVHGIKLLGLCPDNVYAKIISKGERLSIVMMQSLLQAKGHQASLIDPVAYLLANGDYLEAHVDIEASTQNFRQNPLPQDHVNIMPGFTAGNDKGELVTLGRNGSDYSAAVLAACLRAECCEIWTDVDGVYSCDPRLVPDARLLKSLSYQEAMELSYFGASVLHPKTIAPIAQFHIPCLIKNSFNPQGAGSLIGLDTGEDKLDIKGITTLKDLTMVNVSGPGMKGMVGMAARVFGAMSSAGVSIVLITQSSSEYSISFCIESEDKLAAQRALREHFELELKEGLLEPVEFMDNVAIVTLVGDGMRTSRGVASRFFTSLAEVDVNIVAIAQGSSERAISAVIPDDKISESVKACHENLFNSQHFLDVFVIGVGGVGGELVDQIQRQQAKLAENGIVIRVCGLANSKGLLLDSNGLPLENWRDAMGQVTEPLSLARMIQLVKRNHVINPIVIDCTSDQGIAEQYVDFLSAGFHVITPNKKANTASMAYYQQLRQAARSMRRKFMYDTTVGAGLPVIENLQNLMAAGDELERFSGILSGSLSFIFGKLDEGMSFSEATTVARNNGFTEPDPRDDLSGMDVARKLLILAREAGMALELEDVVVEQALPPGFDAEGSVDDFMARLPEADAYFATLIADAASEGKVLRYVGEIKDGQCAVKIAAVNPDDPMF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein with aspartate kinase activity, homoserine dehydrogenase activity is needed to enhance threonine biosynthetic process, lysine biosynthetic process via diaminopimelate, phosphorylation in a specific context.2. We are looking for a protein with broad substrate specificity for L-homoserine + NADP(+) = H(+) + L-aspartate 4-semialdehyde +   NADPH.3. A protein that is capable of catalyzing reactions within the Amino-acid biosynthesis, L-threonine from L-aspartate: step 3/5 pathway with high efficiency and specificity.4. The designed protein should contain one or more ACT domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MRVLKFGGSSLGSAERFVQVAKIIADKSNDSAVAVVVSAPQGVTNHLVAMAENISDEIKLQNDLEHFRKAIITIIDDLGASLPKFSPLHAEQVLINWEHKLSRYLQGATMLSYCPDHIRAIIISIGERLSVALLESVLNAQNIKTSVIEPEKFLITNEASLNAVADLVLSKENFENHYNNLNKVALMPGFIGVNKQGQVTTLGRNGSDYSAAVLAVCAQAECCEIWTDVDGVFNADPRLIKEAKLLDYLSYQEAMELSYFGASVLHPKTIGPIAQFHIPCLIKNTGNPDAPGTLILEGTGLIGVPGTAERVFAALRNAHVSVVMISQGSSEHSICCVVRASEAAKGQAALLDAFAHELDTRQIQRVQELEGTGLIGVPGTAERVFAALRNAHVSVVMISQGSSEHSICCVVRASEAAKGQAALLDAFAHELDTRQIQRVQEGKGNIGASWLRLFYQEKAELEKRRGMNFELVAVVDSQRYVFNEAGLNEQDVFEHYQDSAINHDGQNWLASVDNLHRYDDTIVLDVTASPEVATQYLAFAQHGMHLISANKVAGSAPSDYYNQVKDAFAKIGRHWFYLEGTGLIGVPGTAERVFAALRNAHVSVVMISQGSSEHSICCVVRASEAAKGQAALLDAFAHELDTRQIQRVQEVSGPGMKGMVGMASRVFATMSQEKISLVLISQSSSEYCISFCIHTSDAELAELSLQQEFELELLNGLLEPLKLQVSGPGMKGMVGMASRVFATMSQEKISLVLISQSSSEYCISFCIHTSDAELAELSLQQEFELELLNGLLEPLKLQ",
    "response#2": "MKILKFGGTSLANATKFLTVANIIEKNFKKEQVAVVLSAPENVTNSMIKIIEKIIEERDVSQNIHSVKSIFLDLIFDIYKATKNFSYKTIKKIIDEKFENLTSIFNGIRFLNQCPDNVRAKIVCCGELLSITIMDGILKSMSYNVTIIDPIENLVAQGDYLDSTIDIQTSKKKIRSINIPKNHIILMAGFIAGNKKKKLVVLGRNGSDYSAAILSVCLKGNVCEIWTDVDGIYTCDPKKVKNAKLLKSLSYKEAMEFSYFGAQVLHPKTIFPIKKFKIPCLIKNTKNPNAVGTMIVSGPGMKGMVGMASRVFATMSQEKISLVLISQSSSEYCISFCIHTSDAELAELSLQQEFELELLNGLLEPLKLQLAVGDGMAGQPGVAARLFESLGRAQVNILAIAQGSSERNISVAVDSRHATKALRAAHAGFWLSPQTFSVGVIGPGNVGVTVSGPGLKGVVGMASRVFASMARAQISVSLITQSSSEFSISFCVPQLHLAAAKHALQQELAVGDGMAGTPGVSARLFGALGRAQVNILAIAQGSSERNISVAIDSADATKALRAAHAGFWLSPQTFAIGVIGPGE",
    "response#3": "MRVLKFGGSSLGSAERFVQVAKIIADKSNDSAVAVVVSAPQGVTNHLVAMAENISDEIKLQNDLEHFRKAIITIIDDLGASLPKFSPLHAEQVLINWEHKLSRYLQGATMLSYCPDHIRAIIISIGERLSVALLESVLNAQNIKTSVIEPEKFLITNEASLNAVADLVLSKENFENHYNNLNKVALMPGFIGVNKQGQVTTLGRNGSDYSAAVLAVCAQAECCEIWTDVDGVFNADPRLIKEAKLLDYLSYQEAMELSYFGASVLHPKTIGPIAQFHIPCLIKNTGNPDAPGTLINLEGTGLIGVPGTAERVFAALRNAHVSVVMISQGSSEHSICCVVRASEAAKGQAALLDALGLGNIGQRFLELLPGQLNRIPALENVHLVGLVASNKALLNTDGIDVVRAIKLYERDAKAYDNALLMSWLTNHPYDELIVVDITPSEHFSYLYDDFFREGIHVIGANKWAASSSTKQYNTLVSTAAKNKRP"
  },
  {
    "reference": "MNSEPKCPFHQTAGGGTSNRDWWPGTLRVDLLHQHSAKSDPMGADFDYRKAFQSLDLAAVKKDLHALMTDSQDWWPADFGHYGPLFVRMAWHSAGTYRIGDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPVKQKYGNKLSWADLMILAGNVALDSMGFKTFGFAGGRADVWEPDLDVYWGREDKWLGGDMRYSQGSPGVEESHGVLVKDDDSQVPHTRDLENPLAAVQMGLIYVNPEGPDGKPDPVAAAHDIRETFARMAMDDEETVALIAGGHAFGKTHGAAPAEHVAKEPEACALEEQGFGWRNSFGKGHGGDTITSGLEVTWTTTPTKWSNDYFKHLFGLEWELTRSPAGAQQWVAKGGAGAGTIPHAHDAGKKLAPTMLTTDLSLRLDPAYEKISRRFMDHPDQFADAFARAWFKLTHRDMGPRARYLGADVPAEELIWQDPIPKVDHALVDASDVAALKQKVLASGCTVAQLVSTAWASASTFRGSDKRGGANGARIRLAPQKDWAVNEPEQLAQVLGKLEGIQAEFNKAGGKKVSLADLIVLAGNAAVEKAAKDAGQTVSVPFTPGRMDASQAQTDVASFAPLEPVADGFRNYLKGRYSVPAEALLIDKAQLLTLTAPEMTGLIGGLRVLGANAGGARHGVFTDRPGQLSNDFFVNLLDMKSEWKATGRDAAEFEARDRKSGGVKWTGTRVDLVLGSNAQLRAVAEVYAGADAQAKFVQDFVAAWTKVMNLDRFELGA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein should exhibit Polar residues compositional bias.2. The protein must be engineered to have a cooperative binding mechanism with heme b to ensure proper regulation.3. The designed protein should have a high specificity for heme b binding, with minimal off-target effects.4. The Proton acceptor of the protein must be engineered to enable effective catalysis of AH2 + H2O2 = A + 2 H2O.5. The protein design must be able to perform metal ion binding, heme binding, catalase activity in order to contribute to response to oxidative stress, hydrogen peroxide catabolic process. The designed protein sequence is ",
    "response#1": "ETMIWQDPIDEKTASKLDNDDINELKKQVLDSGLSVSELVRVAWGAAASYRAADKRGGVNGARIALAPQKDWQVNNPVETQKVIKVLSNIQSNFNGGMFNSSRVSLADLIVLAANAAIEKAAKDAGYAVSVPFIPGRADATQAQTDVNSFSLLELSADGFRNYFDKQNSYKSPTEMLIDKADQLNLSVPQMTVLIGGMRVLDANYQGVKHGVFTNQPGTLSNDFFVNLLDMSTVWRKSSEQGIYQGFDRQTGEPKWSATSVDLIFGSNSELRAVAEVYAK",
    "response#2": "ETMIWQDPIDEKTASKLDNDDINELKKQVLDSGLSVSELVRVAWGAAASYRAADKRGGVNGARIALAPQKDWQVNNPVETQKVIKVLSNIQSNFNGGMFNSSRVSLADLIVLAANAAIEKAAKDAGYAVSVPFIPGRADATQAQTDVNSFSLLELSADGFRNYFDKQNSYKSPTEMLIDKADQLNLSVPQMTVLIGGMRVLDANYQGVKHGVFTNQPGTLSNDFFVNLLDMSTVWRKSSEQGIYQGFDRQTGEPKWSATSVDLIFGSNSELRAVAEVYAN",
    "response#3": "LSWGDLMILAGNVAMEDMGFKTLGFAGGRIDAWQPDLVFWGPEAKLLADERRDAKGNLKGPLAATQMGLIYVNPEGPNGNPDPLLAAADIRRAFGAMNMTDEETAALIAGGHTFGKAHGAHKPEGCVGADPGQGKIEQQGLGWANSCGKGKAEDAVTSGLEGAWSANPIAFTTQYLDNLYGFEWVKTKSPAGATQWIPKDPKAANLVPDAHRPDVRHAPIMFTTDIALREDPGFRKITMAWKKNPQAFADAFARAWFKLTHRDMGPQARYLGS"
  },
  {
    "reference": "MGGKLSKRRIPGWDAIREKMRRTRPEPAAEGVGAVSRDLEQRGAITINNTASNNAACTWLEAQEEEEEVGFPVRPQVPLRPMTYKGALDLSHFLKEKGGLEGLIWSQKRQDILDLWVYHTQGYFPDWDNYTPGPGIRYPLTFGWCFKLVPVDPDWVEETTAGEDNCLLHPMNQHGMDDPDSKVLEWRFDSRLAFHHLARDKHPEYYKNC",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit SH3 domain binding.2. The protein design should include a Dileucine internalization motif; necessary for CD4 internalization motif to ensure proper function.3. For general function, the protein need meet that Inhibits the Fas and TNFR- mediated death signals by blocking MAP3K5/ASK1 The designed protein sequence is ",
    "response#1": "MPFGL",
    "response#2": "MS",
    "response#3": "MKTFL"
  },
  {
    "reference": "MGGGERYNIPDRPAPKKSQPVSRGKQRSRDQNGVMHSAASGALGVPHHLRRGEKGTSYSWSPEARQAVSVDKKNGSVRFATPYDQNWESHLNKLLSAQCGQNYAGAKFSEPPSPSVLPKPPSHWVSLPMGDHRELMTFQLKSLLKVQA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Involved in nonsense-mediated mRNA decay (NMD) by acting as a bridge between the mRNA decapping complex and the NMD machinery; May act by targeting the NMD machinery to the P-body and recruiting the decapping machinery to aberrant mRNAs; Required for upf1/rent1 localization to the P-body2. The protein should have a specific function in nuclear-transcribed mRNA catabolic process, nonsense-mediated decay and should be localized to P-body in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "MHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYR",
    "response#2": "LKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIVEAKLKISDVNCAVHLCSLFHQRYADFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTDKEGLSLIYEQLKNIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQPFQNLLKEYFTSLI",
    "response#3": "MHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYK"
  },
  {
    "reference": "MAKFIFVTGGVVSSLGKGITAASLGRLLKSRGLKVAIQKLDPYINVDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFIDTNLNKTSNVTAGGIYWSVISKERRGDYLGGTVQVIPHITNEIKDRVLRMARELDADVVITEIGGTVGDIESLPFLEAIRQLRTDLGRDNVMYIHVTLMPYLRMANEFKTKPTQHSVKELRSLGIQPDVIVCRTEKPFPKEMEEKLALFCDIDKDAVIQAVDAFSIYEVPLQMEQEGLDDIAVERLGLKCGEADMTGWRDMVNRMKNLKNSTTIALVGKYVSLPDAYLSVAEALRHAGLHHDSAVEIRWVNSEDLEKVPAEQLLKGADGILVPGGFGDRGIEGKINAIRYARENKIPFLGICLGMQLAVVEFSRAVLGWQDANSAEFNPHCQYPVIDLLPEQKDLDKMGGTMRLGAYPCKLAPGTLAYQAYGEEIIYERHRHRYELNNEYRADLAQAGMVFSGTLPNGKLVEIVELPDHPWFLATQFHPEFKSRPNRPHPVFRDFVGAAIKNRRSC",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein contains novel CTP synthase N-terminal, Glutamine amidotransferase domains that confer a unique function or activity.2. The protein with the reaction H2O + L-glutamine = L-glutamate + NH4(+), the Nucleophile; for glutamine hydrolysis, active site must be optimized to ensure efficient catalysis.3. The L-glutamine, UTP, Mg(2+), CTP, ATP binding site should be located in a region of the protein that is accessible to the ligand.4. The designed protein should interact with enzymes and substrates within the CTP from UDP: step 2/2, Pyrimidine metabolism pathway to enhance or regulate its activity.5. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates..6. A protein with ATP binding, glutaminase activity, CTP synthase activity, metal ion binding is needed to enhance glutamine metabolic process, 'de novo' CTP biosynthetic process in a specific context. The designed protein sequence is ",
    "response#1": "MFITGGVVSSLGKGIAAASLAAVLEARGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFVCMTATRRNNFTTGRIYENVIRKERRGDYLGGTVQVIPHITDEIKRCIRLGAGEADIAMIEIGGTVGDIESLPFLEAIRQMGVELGRERSLFIHLTLVPFIKSSGELKTKPTQHSVKELRSIGIQPDILMCRSDREIPAEERRKIALFTNVEPKAVINVPDADTIYRIPLLLRAQGVDDIVARKLQVDIAMVGKYVDLTDAYKSLNEALLHAGIHAHTRVNIHYIDSERVERDGTACLEHMDAILVPGGFGERGIEGKIAAVRFAREHRLPYLGICLGMQVAVIEFARHVAGLEGAHSTEFRKDTPHPVIALITEWIDEDGSVQQRREGGDLGGSMRLGAQPCRLVADSLAGATYGKDVIVERHRHRYEFNNRYRGPLGAVGMRFCGHSLDGRLVEMIELPDHPWFLACQFHPEFTSTPRAGHPLFRGFLNAAVAQRTLPDAYLSVTEALRAGGFASRAKVNIRWVASDECETAAGAAAALRDVDAILIPGGFGIRGIEGKVGAIHFARTRGIPLLGLCLGLQCVVIEAARSIGLTDANSTEFEPDTQHPVISTMADQKQAVAGEADLGGTMRLGAYPAVLEKGSVVARAYGSEQVSERHRHRYEVNNAYRDKIATSGLKFSGTSPDGLLVEFVELPAEVHPFFVATQAHPELKSRPTRPHPLFAALRAAAG",
    "response#2": "MFITGGVVSSLGKGIAAASLAAVLEARGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFVCMTATRRNNFTTGRIYENVIRKERRGDYLGGTVQVIPHITDEIKRCIRLGAGEADIAMIEIGGTVGDIESLPFLEAIRQMGVELGRERSLFIHLTLVPFIKSSGELKTKPTQHSVKELRSIGIQPDILMCRSDREIPAEERRKIALFTNVEPKAVINVPDADTIYRIPLLLRAQGVDDIVARKLQVRPALATARGPLSDAYLSLVEALRHAAIAQGSEIKLRWVNAEKVEQTNPETYLKDVSGIIVPGGFGIRGIEGKIQAVAYARTENIPFLGLCLGMHCAVVEWARNIAKLDRASSAEFNLDTPNPVINLLPEQQDVVDLGGTMRLGLYPSRLNVESHAYSFYQEEVIYERHRHRYEFNNAYRSLFLENGYAIGGTSLDGRLVEMIERSDHAFFIATQFHPEFRSRPNQPHPLFLGFIRACVQGSK",
    "response#3": "MFITGGVVSSLGKGIAAASLAAVLEARGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTHDGAETDLDLGHYERFVCMTATRRNNFTTGRIYENVIRKERRGDYLGGTVQVIPHITDEIKRCIRLGAGEADIAMIEIGGTVGDIESLPFLEAIRQMGVELGRERSLFIHLTLVPFIKSSGELKTKPTQHSVKELRSIGIQPDILMCRSDREIPAEERRKIALFTNVEPKAVINVPDADTIYRIPLLLRAQGVDDIVARKLQLPGLFPEVVEQDRALSDAYLSLVEALRHAAIAQGSEIKLRWVNAEKVEQTNPETYLKDVSGIIVPGGFGIRGIEGKIQAVAYARTENIPFLGLCLGMHCAVVEWARNIAKLDRASSAEFNLDTPNPVINLLPEQQDVVDLGGTMRLGLYPSRLNVESHAYSFYQEEVIYERHRHRYEFNNAYRSLFLENGYAIGGTSLDGRLVEMIERSDHAFFIATQFHPEFRSRPNQPHPLFLGFIRTAIASRDGRPVKIAIVGKYTGLKDAYKSLQEALVHGGIANNVRVNITWLASEIFEQDHEAILALEDVNAILVPGGFGERGTSGKIAAVQFARERKVPYLGICYGMQMAVIEAARNLAGLKDANSTEIGPTRNPVVGLMTEWVRGNRTELRQEGGDLGGTMRLGAYDAVLSEGSRVAEIYGTRLISERHRHRYEVNPAYRRHLEEHGVLFSGMSPDGILPEVIEIKDHPWFIGVQFHPELKSKPLDPHPLFSSFLQAALDHQDQP"
  },
  {
    "reference": "MSTPSGSNPLPMADKLEAKGRIASIKFDYVKNGQPKAGSTHGVSYHNFTEWFDLNHTCDEHILSVKCYYDDGEIQGLVIKTNIRTSAYMGYNIGTTFTLEVKGKKIVGFHGSFDKNLTSLGAYFAPLSPAK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein have Jacalin-type lectin domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "IWDANRNNPVGENATLSLGRNGNLVLAEADGRVKWQTNTANKGVTGFQILPNGNIVLHDKNGKFVWQSFDHPTDTLLTGQSLKVNGVNKI",
    "response#2": "GIPWDHGPNHNGVKRVSFIFDENEIRQWRVDYDDGGVIRQYEPINGYDMFEVKEYPTEYIISVECTYDDVIPRSGRRMIRSIMFKTSKGRVSPIFGYPAARKFVLENNGGALIGFHGRVGAGIDALGAYFSD",
    "response#3": "WDDKSDHDDVTKIYVNYSLMGIESIRFDYVKSGKPIEGPFRGETYNTYTHTFEINHLKNEHLESVEGSYTQRGIQTLQFKTNLRISEPIGYPGKDGIKFILAVEGKKIIGFHGSTYFRLYSLGAYFE"
  },
  {
    "reference": "MENRWQVMIVWQVDRMRINKWKSLVKYHMHISKKAGRWFYRHHYESNHPRISSEVHIPLGEARLVVTTYWGLLTGEREWHLGQGVSIEWRLRRYRTQVDPGLADQLIHLYYFDCFTDSAIRKAILGHRVSPRCEYQAGHNQVGSLQYLALRALVNPKKTKPPLPSVRKLTEDRWNKPQKTRGHRENQTMNGH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be expressed specifically in the host cell plasma membrane, host cell cytoplasm, plasma membrane2. The protein must exhibit the following characteristics: May interact directly with APOBEC3G mRNA in order to inhibit its translation; Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism. The designed protein sequence is ",
    "response#1": "EGGVPN",
    "response#2": "ECCPG",
    "response#3": "GFAK"
  },
  {
    "reference": "MAVYTDVAAEELADFLKAYDIGELLSYKGIAEGVENSNYLLHTTSGSFFLTLYEKRVAIDDLPFFLGLMGHLATHGIVCPQPVRTRNGETLGSLAGRPAAIIDFLEGVWPRKPNVAHCAAVGAALAKLHLAGRDFSMRRANALSVWSWRPLFEQAGACADTVQPGLHDFLKAELDHLEARWPQELPGGVIHADLFPDNVLFLGDRLSGLIDFYFACHDFFAYDVAICLNAWCFEPDHAFNVTKARALLGAYHRERALSEAEQAALPLLARGAALRFLLTRLVDFLNVPAGALVKPKDPLEYVRKLRFQQRVGGVRDYGVAILGPVA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design must be able to perform homoserine kinase activity, ATP binding in order to contribute to threonine biosynthetic process, phosphorylation.2. The protein should have a low Km value for ATP + L-homoserine = ADP + H(+) + O-phospho-L-homoserine.3. Ideal protein should have a binding affinity for key enzymes in the Amino-acid biosynthesis, L-threonine biosynthesis, L-threonine from L-aspartate: step 4/5 pathway to enhance the overall efficiency of the pathway. The designed protein sequence is ",
    "response#1": "LQGIASGITNTNYFVATGTGRYVLTLFEEHSAEELPNFLDLMTHLAERGIPCPYPVKNIAGQALGELNGKPAALVSCLAGKSLDNPSPQHCAAIGEVLARMHLAGASFEVSIINLRCQRWRIARLPRWAPFLDAENXRMLDAQLEFEQAFDTSALSRGVIHADLFRDNVLMDGDKVGGVLDFYYAWNDVLFYDIAIAVXDWCVNADGTRDVGRVRAFLDAYHAVRPDL",
    "response#2": "MAATEDVLHQPQRAAAMPASAEYLAILRRCGIAAVLSGAGPSVLGLSAQSGLPAEALLAPL",
    "response#3": "FKGIAEGVENSNFLLETEKGRYILTVYERRVKAEDLPYFLNMLTWLADRGYPSARPIPTRSGATLSSLRGKPAAIVEFLPGLSVRKPTAAHCREAGEGLAWLHLAGEGYPGRRANDLGQAAWSPLFSKHRKAAEDLKPGLSATIDNDLAQLSLMWPRNLPTGTIHADYFPDNVFFQSNGKFAAAIDFYFACDDAYAYDVAVTLNAWCFEADGSFNITAAKALLNGYERRRPLQGIASGITNTNYFVATGTGRYVLTLFEEHSAEELPNFLDLMTHLAERGIPCPYPVKNIAGQALGELNGKPAALVSCLAGKSLDNPSPQHCAAIGEVLARMHLAGASFEVSIINLRCQRWRIARLPRWAPFLDAENXRMLDAQLEFEQAFDTSALSRGVIHADLFRDNVLMDGDKVGGVLDFYYAWNDVLFYDIAIAVXDWCVNADGTRDVGRVRAFLDAYHAVRPE"
  },
  {
    "reference": "MLSSKRILLIITGGIAAYKSLDLIRKLRAAGAAVTPVLTGSAQEFVTPLSVAALAGEAVHSELFDLTSEAEMGHIELSRSADLILVAPATADMMAKMAQGRADDLASTLLLATDTPVLIAPSMNVRMWEHSATXRNLRQLQADGVKIIGPEAGDMACGEFGPGRMSEPVDILTAVQMHFTRGPLAGQRVLVTSGPTHEPIDPVRYIANRSSGRQGSAIAQALVNQGADVVFISGPAAADPPLGAQIKRVETALEMAQAVEQALPCKIAIFVAAVADYRVELPGDEKIKKQAGGLSTLSFVENPDILASVAQRRDNRPDLVIGFAAETEALEENAQAKRMRKGCDWLLANDVSAKTGIMGGDENAILCIEQSGVDQWPRASKAQVAERLVQKIIDHVEKASTAHG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Mg(2+) is required for the proper folding and stability of the protein.2. The desired protein should be able to perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO23. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.4. The protein design must be able to perform FMN binding in order to contribute to coenzyme A biosynthetic process.5. We require a protein that can specifically bind to the metabolites produced in the coenzyme A biosynthesis, Cofactor biosynthesis pathway. The designed protein sequence is ",
    "response#1": "MNIVLGVTGGIAAYKAVALARLFVEAGHEVHVIPTADALRFVGAPTWEAISRNPVTTSVHEDVPEVRHVALGQRADVVVVAPATANTLARMTAGLADDLLGTTLLATRAPVVVAPAMHTEMWQHPATQDNVAILRARGVHFIGPESGRLTGDDTGPGRMSEPEEIFAEVRELLLRLLTPKQDLEGKRFLVSAGGTREPIDPVRYIGNRSSGKQGVAVAAAAAALGAKVVLLAANVDEAVLAEVAENPAVRVVPVGSAAELQSAALAASSGADVVIMAAAVSDYRVADVAEEKIRKEDSAGEPPTLTLVENPDILAELVHMRRPGQLLVGFAAETAASTDELLERGRRKLRRKGVDLLAVNAVGWNEGFESAENTLHIVGRDGVPVELPLMTKREIADRVLDAVHALAAR",
    "response#2": "MNIVLGVTGGIAAYKAVALARLFVEAGHEVHVIPTADALRFVGAPTWEAISRNPVTTSVHEDVPEVRHVALGQRADVVVVAPATANTLARMTAGLADDLLGTTLLATRAPVVVAPAMHTEMWQHPATQDNVAILRARGVHFIGPESGRLTGDDTGPGRMSEPEEIFAEVRELLGPDRPPRLAGRHVVVTAGGTREPLDPVRFLGNRSSGKQGYALARAAVARGARVTLVAANVTLTDPAGADVVRVGTTEELRKATVEAAGGADVVVMAAAPADFRPATVAGQKIKKSDEGTAPTIELVTNPDIAAELGAAKRPGQLLVAFAAETHDALENARGKLVKKRADLIVVNEVGVDLVFGADHNTVTILGADGSGTRLAHGSKTLMASRVLDEAARLRGGR",
    "response#3": "MNIVLGVTGGIAAYKAVALARLFVEAGHEVHVIPTADALRFVGAPTWEAISRNPVTTSVHEDVPEVRHVALGQRADVVVVAPATANTLARMTAGLADDLLGTTLLATRAPVVVAPAMHTEMWQHPATQDNVAILRARGVHFIGPESGRLTGDDTGPGRMSEPEEIFAEVRALAAAALSPRALEGKRFLVSAGGTREPIDPVRYIGNRSSGKQGVAVAAAAAALGAKVVLLAANVDEAVLAEVAENPAVRVVPVGSAAELQSAALAASSGADVVIMAAAVSDYRVADVAEEKIRKEDSAGEPPTLTLVENPDILAELVHMRRPGQLLVGFAAETAASTDELLERGRRKLRRKGVDLLAVNAVGWNEGFESAENTLHILWPEGRVEELPLLTKGEVAEAILDRVLRLRAL"
  },
  {
    "reference": "MVAFLFCNLLLVACGSVTWTMSDTGESGVDLADRLDLVEKIGDTHSKDLEIWMELGKQREADAREMHAVCRVQPSAMLPPDQPQITGLVLFRQLGPSSRLEASFNLEGFPAEQNTSNHAIHVHEFGDLSQGCESTGPHYNPLGVPHPQHPGDFGNFVVRDGRLWKHRMGLATSLAGPHSILGRAVVVHAGEDDLGKGGNQASVQNGNAGRRLACCVVGTSNSEAWESQTKERKKRRRESECKTT",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. Include a signal peptide as an essential component of the protein.2. The protein must be engineered to have a cooperative binding mechanism with Zn(2+) to ensure proper regulation.3. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics.4. The protein should be designed to catalyze the following reaction 2 H(+) + 2 superoxide = H2O2 + O2.5. The ideal protein would be one that plays a critical role in removal of superoxide radicals through superoxide dismutase activity, copper ion binding within Golgi apparatus, extracellular space, collagen-containing extracellular matrix.6. The protein must exhibit the following characteristics: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen..7. The designed protein should be able to compete with endogenous proteins or inhibitors for Cu cation, Zn(2+) binding. The designed protein sequence is ",
    "response#1": "VGGTVVFEQESESAPTTITWDITGNDANAKRGFHIHTFGDNTNGCTSAGPHFNPHGKTHGAPTDEARHVGDLGNLETDGQGNAKGSVKDEHVKLIGPHSVIGRTVVIHAGTDDLGKGDNEESLKTGNAGPRPACGVAA",
    "response#2": "TGYVTLTAQDGALNLVYNISSINLPTGSYATAIMTYGYNPSNNTDLGGVFQVDGKGTEQCQSGGSRAGDLYNLYVNDNRISSNFDSLTSVSIVDSPNSIIGRSIAIFQP",
    "response#3": "VSGVVKFEQASESEPTTVSYEIAGNSPNAERGFHIHEFGDATNGCVSAGPHFNPFKKTHGAPTDEVRHVGDMGNVKTDENGVAKGSFKDSLIKLIGPTSVVGRSVVIHAGQDDLGKGDTEESLKTGNAGPRPACGVA"
  },
  {
    "reference": "MVAETGTGLFSAHKITEELASTSLDSGVHLLFDIHDRTFQALPDFLAEHRYQEVNDIRNTVFQKAFDTNLSIYEYLVHHPQLQAHMQDAMKLHQPEGDWLSVFPADEIVGNQQTAPDPARVLFVDIGGGMGQQCIRFRERYPDLAGRVILQDIPQTINRVPKPMPNGIEAVPHSFEDPQPIKSKSPRLDNLARERL",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.2. A protein that is capable of catalyzing reactions within the terpenoid biosynthesis, Secondary metabolite biosynthesis pathway with high efficiency and specificity.3. The designed protein must be able to regulate methylation, terpenoid biosynthetic process.4. The protein should have a high affinity for S-adenosyl-L-methionine.5. For general function, the protein need meet that The first step of the pathway is the synthesis of the alpha-pyrone moiety by the polyketide synthase dpmpA via condensation of one acetyl-CoA starter unit with 3 malonyl-CoA units and 2 methylations (Probable); The short chain dehydrogenase/reductase dpmpG then oxidizes the 8S hydroxy group to a ketone and the short chain dehydrogenase/reductase dpmpH reduces the ketone to the 8R hydroxy group to yield higginsianin B; Subsequent steps in the diterpenoid pyrone biosynthetic pathway involve the decalin core formation, which is initiated by the epoxidation of the C10-C11 olefin by the FAD-dependent oxidoreductase dpmpE, and is followed by a cyclization cascade catalyzed by the terpene cyclase dpmpB (Probable) The designed protein sequence is ",
    "response#1": "DESIAIVGASCRFAGSETPAGFWDTLRERRSTLGKAPVWRGYGSEEEPFWGNFLASAGAFDHAFFRKSPREAAYMDPQHRLALHLAYEALESGGYFNPAAGTQTYDVGCYVGVYSSDYEDNVNARPPTPFSFTGTARAFASGRISQFFGWTGPSLIVDTACSSSGVAIHTACKAIQSGECTMALAGGVNLMTSPKSHQNLAAASLMSRTGQCKPFDASADGYCRGEGGGFVLLKRLSSAVADNDRVLGVLAASAINNSKGSLTITAPSLESQAALYQSVLRKAGMQPDQVSYVEAHGTGTQKGDPVECHSLRRVFGRSSRNSPPLRFGSVKGNIGHSEGASGVASLVKVLLMLQHGLIAPQANFSVLNPAAPNLEEANMEIPLYLQPWDAAFRAACEDESIAIVGASCRFAGSETPAGFWDTLRERRSTLGKAPVWRGYGSEEEPFWGNFLASAGAFDHAFFRKSPREAAYMDPQHRLALHLAYEALESGGYFNPAAGTQTYDVGCYVGVYSSDYEDNVNARPPTPFSFTGTARAFASGRISQFFGWTGPSLIVDTACSSSGVAIHTACKAIQSGECTMALAGGVNLMTSPKSHQNLAAASLMSRTGQCKPFDASADGYCRGEGGGFVLLKRLSSAVADNDRVLGVLAASAINNSKGSLTITAPSLESQAALYQSVLRKAGMQPDQVSYVEAHGTGTQKGDPVECHSLRRVFGRSSRNSPPLRFGSVKGNIGHSEGASGVASLVKVLLMLQHGLIAPQANFSVLNPAAPNLEEANMEIPLYLQPWDAAFRAAC",
    "response#2": "VAIIGMACRTPGDVRSPDSLWQYLLKKGDASGSLPDWRWEPYRQRHPRNAALLAQTTAKGYFLDDIDHFDAAFFSISPREAEQMDPQQRLALEVAWEALENAGISPPQLAGSNTSVYMGVNSDDYAKLLLEDLPNVDAHMGVGTAYCGIPSRISYILDLMGPSVALDAACASSLVAVHHARQAIRAGETDLAIAGGVNALLGPGLTRVLDEAGAISTDGKCRSFDETASGYGRGEGAGVVILKRLDKALADGDHVLAVLKGSAVASDGKTLGIMAPNARAQLLVAQKALAEAKVSADSINYVEAHATSTSLGDPTETNALAEVYGAGSGRSPSDPCYIGSIKPNIGHLEAGAGVMGLIKAVLVLRHGQVPPQANLKTLNSKIAWNENLLCPPRELVTLPCPGPIHPLRAAVASYGYSGTVSHAVLEAFADYADRL",
    "response#3": "DESIAIVGASCRFAGSETPAGFWDTLRERRSTLGKAPVWRGYGSEEEPFWGNFLASAGAFDHAFFRKSPREAAYMDPQHRLALHLAYEALESGGYFNPAAGTQTYDVGCYVGVYSSDYEDNVNARPPTPFSFTGTARAFASGRISQFFGWTGPSLIVDTACSSSGVAIHTACKAIQSGECTMALAGGVNLMTSPKSHQNLAAASLMSRTGQCKPFDASADGYCRGEGGGFVLLKRLSSAVADNDRVLGVLAASAINNSKGSLTITAPSLESQAALYQSVLRKAGMQPDQVSYVEAHGTGTQKGDPVECHSLRRVFGRSSRNSPPLRFGSVKGNIGHSEGASGVASLVKVLLMLQHGLIAPQANFSVLNPAAPNLEEANMEIPLYLQPWDAAFRAACQLDLDAVGDPGGPAGPPRVLRPLVLPAESRTAADTGWLAELDRLSPAVGPAVRGRGDR"
  },
  {
    "reference": "MVNIKEKLFVYLHDPPDKALKIENHEERSKKILSSGNIQYSRTDKVKQADALSSKTQRFIIRTKENKEPVIDFLGRSSGKYFHVGYPVFIHPISTEIKRYETLEKYIDLGRSNRGERFVNEFLERVSKLEGDVLKEVFEDASNKFKGEESKQWAYIWQFYPVKLKEGVKEFAKSELKLKEEEAEKFAEEFVNLPADTRFPDHAIWTHLDLTSALSVKDPTLLRIKIVPVQPFIANSRKQLDLWASSHLLSMLMYKALEVIVDKFGPEHVIYPSLRDQPFFLKFYLGENIGDEILVANLPNKALAIVSGKEAEKIEEEIKKRIRDFLLQLYREAVDWAVENGVVKVDRSEKDSMLKEAYLKIVREYFTVSITWVSLSEKEDIYQVTENAGLSDEDVKKWLKFAEKKENSRVLERIAIYPLLVKILDSLGERKVTEERFEKSEQLKGWKCHVCGENLAIFGDMYDHDNLKSLWLDEEPLCPMCLIKRYYPVWIRSKTGQKIRFESVVDVALLYKNWRKIFDEKYGKDLVSKAREVSEDFVKDNMLVDSDLYYSSTWESGLSKKLKNKKEIDEEKVKEVVDFLNAAYKEIGNPPKYYAILVMDGDDMGKVISGEVLGEISTRIHPNIRDYVEIPEAKYYSTPQVHVAISQALANFSIREVRSVVKDEGLLIYAGGDDVLAILPVDKALEVAYKIRKEFGKSFENGSLLPGWKLSAGILIVHYKHPLYDALEKARDLLNNKAKNVPGKDTLAIGLLKRSGSYYISLVGWELI",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be involved in defense response to virus by localizing to cytoplasm and exhibiting metal ion binding, RNA binding, nucleotide binding.2. The presence of Zn(2+) is crucial for the protein to function optimally under specific conditions.3. The designed protein must have at least one GGDEF domain.4. For general function, the protein need meet that Cmr complex does not cleave ssDNA complementary to the crRNA; Cleavage of target RNA is guided by the crRNA; substrate cleavage occurs a fixed distance (14 nt) from the 3' end of the crRNA; CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids; Part of the Cmr ribonucleoprotein complex which has divalent cation- dependent endoribonuclease activity specific for ssRNA complementary to the crRNA (target RNA), generating 5' hydroxy- and 3' phosphate or 2'- 3' cyclic phosphate termini; In vitro reconstitution shows Cmr1-2 and Cmr5 are not absolutely necessary for target cleavage.; CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), formerly called psiRNA (prokaryotic silencing) in this organism5. The Zn(2+), Mn(2+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "SERMADERDKANRRRYNDNQEAMKKIQRDYGKEGYISRGDIVRLDALELQGCACLYCGTTIGYHTCQLDHIVPQAGPGSNNRRGNLVAVCERCNRSKSNTPFAVWAQKCGIPHVGVKEAIGRVRGWRKQTPNTSSEDLTRLKKEVIARLRRTQEDPEIDERSE",
    "response#2": "SERMADERDKANRRRYNDNQEAMKKIQRDYGKEGYISRGDIVRLDALELQGCACLYCGTTIGYHTCQLDHIVPQAGPGSNNRRGNLVAVCERCNRSKSNTPFAVWAQKCGIPHVGVKEAIGRVRGWRKQTPNTSSEDLTRLKKEVIARLRRTQEDPEIDERSLQQRISEAGSAVSRVAPDPLPGQGSFRRAPKCDPEFQRFRIISIVANLRISETKGENRPLTADERRHVVTFLTEDSQADLTWVDVAEKLGVHRRDLRGTAVHTDDGERSAARPPIDATDRIMRQTKISSLKTWWEEADSEQRGAMIRYLYEDPTDSECAEIIAELPEEDQAKLDSLHLPAGRAAYSRESLTALSDHMLATTDDLHEARKRLFGVDDSWAPPAP",
    "response#3": "SERMADERDKANRRRYNDNQEAMKKIQRDYGKEGYISRGDIVRLDALELQGCACLYCGTTIGYHTCQLDHIVPQAGPGSNNRRGNLVAVCERCNRSKSNTPFAVWAQKCGIPHVGVKEAIGRVRGWRKQTPNTSSEDLTRLKKEVIARLRRTQEDPEIDERSGFSSSPVRGLSSERMADERDKANRRRYNDNQEAMKKIQRDYGKEGYISRGDIVRLDALELQGCACLYCGTTIGYHTCQLDHIVPQAGPGSNNRRGNLVAVCERCNRSKSNTPFAVWAQKCGIPHVGVKEAIGRVRGWRKQTPNTSSEDLTRLKKEVIARLRRTQEDPEIDERSGFSSSPVRGLSSPIRGLSPSPRGLSSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPIPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPIPVRGLSPSPVRGLSPSPVRGLSPSPVRGLSPSP"
  },
  {
    "reference": "MTKLTPMMRQYLDIKAQYPDAILFFRLGDFYEMFMEDAVTASRILDITLTSRNKGAAEEVPLCGIPYHSCQPYVARLVEAGHKVAICEQVEDPKTVKGIVKREVVRVVTPGLVVDADTLTPKENNFLAAIAVSAQERYGIAVLDITTGEFRVTEVEGREAACSEVVSLQPREILVSEDQQQFCEQLLQGASGSWLINPLPDWVCDLEACGQRLQSFFNCGSLESFGCARLPEAVRAAGGVVYYVEQTQKGVAGHIRPLVTYHSRDYMVLDNSTRRNLELTATLQDGGRKGSLLGVLDRTVTAMGGRKIRQWIHHPLVDLKAIRDRHLSVQELVGQSLVRGDLRADLDGVYDLERLNSKIAMGHANAKDLVALRKSFEKLPRILQHLDELSAPLAAGIGSRIDPLTDMAELIGRAIVEDPPFVLREGGLMCDGYHLELDELRDIRRNGKEWIAKLEADERERTGIGSLKVRFNKVFGYYIEVTRTHLGRVPEDYQRKQTLANAERFFTPQLKEYEEKVLGAEDRLFDLEFELFQDLRERVAEQGERVQRTAEALAELDVLLSLADVAHSCDYVCPTMDDSDRLVIRDGRHPVIEAMNLGEHFVPNDVEMDCRENQIMVITGPNMAGKSTYMRQVALITLMAHMGSLVPAASAHIGLVDRIFTRVGASDNLAQGQSTFMVEMTEAAHILNHATSRSLIVLDEIGRGTSTFDGISIAWAVAEYLHDNGHVAAKTLFATHYHELTDLILTCERVKNLNIAVREWNEQIIFLR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that This protein has a weak ATPase activity.; This protein is involved in the repair of mismatches in DNA2. A protein with damaged DNA binding is needed to enhance mismatch repair in a specific context.3. The designed protein should have a binding site that is specific to the target ATP ligand. The designed protein sequence is ",
    "response#1": "MVITGPNMAGKSTVMRQVALTALMAQAGSFVPAKAARIGLCDRIFTRVGAADNLARGQSTFMVEMTETSHILHHATNKSLIILDEIGRGTSTFDGLSIAWAVAEHLHDTVGARALFATHYHELVDLARERPRVKNLCVAVS",
    "response#2": "KYFCLITGPNMAGKSTYLRQVALITLMAHIGSFVPASRAVIGITDKIFCRIGASDNLAKGESTFLVEMNETANILRNATEKSLIIMDEVGRGTSTNDGLAIAYSIIEYILEYIKARSLFATHFHELSAINHKAFINLSMKIEKQGNELVFLREVEEKPSLNSYGIYVS",
    "response#3": "MVITGPNMAGKSTVMRQVALTALMAQAGSFVPAKAARIGLCDRIFTRVGAADNLARGQSTFMVEMTETSHILHHATNKSLIILDEIGRGTSTFDGLSIAWAVAEHLHDTVGARALFATHYHELVDLARERPRVKNLCVAVN"
  },
  {
    "reference": "MIRSVLASVSPLRQILASQLCTSSITLAGIRPLGPKNKAPDFSGTAVVNGDFKTISMKDYKGKWLILFFYPLDFTFVCPTEITAFSDRCAEFQKLNTELIACSCDSHFSHLAWIQTPRSEVGGLGDMKIPVLADFNKDIANAFGVLDHETGISYRGLFLIDPSGEIRHSLVNDLSVGRSVDEAFRTLKAFQFVEKHGEVCPANWSDDKPTIKPGIKESKEYFKKVDGHT",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must be optimized for thioredoxin-dependent peroxiredoxin activity.2. For general function, the protein need meet that Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively The designed protein sequence is ",
    "response#1": "MFPKTLTDSKYKAFVDGEIKEISLQDYIGKYVVLAFYPLDFTFVCPTEINRFSDLKGAFLRRNAVVLLISCDSVYTHKAWASIPREQNGVLGTAWPMVWDAKRELCNQFGLYDEENGHPMRSTVILAKDLSVRHISSNYHAIGRSVDEIIRLIDAITG",
    "response#2": "MFPKTLTDSKYKAFVDGEIKEISLQDYIGKYVVLAFYPLDFTFVCPTEINRFSDLKGAFLRRNAVVLLISCDSVYTHKAWASIPREQNGVLGTAWPMVWDAKRELCNQFGLYDEENGHPMRSTVILAKDLSVRHISSNYHAIGRSVDEIIRLIDAITC",
    "response#3": "PPAFTLKDQDGKNVSLSKFKGKPVVVYFYPADETPGCTKQACAFRDSYEKFKKAGAEVIGISGDDSSSHKAFKQKYKLPYTLLSDEGNKVRKDWGVPSDLFGALPGRQTYVLDRNGVVRL"
  },
  {
    "reference": "MTTTTQIASHLLRSRIGKICVAITGSTPEEFLEKATEVLKETTFLEFRLDYLPKPLAALPKFKAYLAENTAATVVATCRCKDNGGRFEGSAAAALEVLIKAAEAGFQLVDIELEAIEKLPKTTVDKLREAGAAVIISHHDFQATKDLDAIYKRTEPFAPDFVKVCPTARSLSDNLTLMRFLEKMEDHPGSSVVGICMGDAGIISRVLGLRAGSAFTFAAASQGEETAPGQIAARTLIETYRIDQVDAATKVYGVAGNPIRNSLSPLMMNTAFRRETVNAVYLALQTSKADDLFKLMREIPIQGLSVTMPLKIDVIPFLERTDPLSQKIGAVNTILRAQDGKFYGFNTDVAGILNPLERRISLKNAKVLVLGAGGAARAAVFGCRDKGAEVSILNRTPETAQKLAKQAGAKVIKREAVAKAGFDVIINATPIGMAGNKTPLPLGPEDLVARYVFDLVYNPIETPLLRMAKQKGIHVISGVEMFVQQGARQFEIWTGKPAPEEEMLRVVVHALRQSTENVEGSIAPTLKTTVVHAAEPAEPPAAKEPEAAPVEVAKKAAPVKAPAKVEAAKSAAKPVSPKPVAARVPVTTAKPAVKAPAKTSAKPAPKPAAKKQASKPAAKPVLAKKVSKPVAKPVAKKAAKKTAAKPAPKKAAKTVAKSVAKKVAKPVAKRPAAKSKPAAKKAKSR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should have a high affinity for shikimate, NADP(+), 3-dehydroquinate.2. A key design requirement for the protein is the optimization of the Schiff-base intermediate with substrate, Proton acceptor to achieve high catalytic efficiency for NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH.3. For general function, the protein need meet that Catalyzes the cis- dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate.; Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids4. A protein that can modulate the overall flux of metabolites through the Metabolic intermediate biosynthesis, chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7, chorismate biosynthesis pathway, enhancing or suppressing its activity as needed.5. The protein design should emphasize Basic residues compositional bias.6. A protein with shikimate 3-dehydrogenase (NADP+) activity, NADP binding is needed to enhance aromatic amino acid family biosynthetic process, 3,4-dihydroxybenzoate biosynthetic process, chorismate biosynthetic process, amino acid biosynthetic process, shikimate metabolic process in a specific context. The designed protein sequence is ",
    "response#1": "MRLTGKKVLLLGAGGVARAIGVGAKDRGAELLVTSRTYERAVELAAKLEGEALAWEDRHSANADVLVNCTPVGMHPKMNETPFEEAL",
    "response#2": "MSDLLIGDPVDKSISDDTHNILISEWKFDAVYVKILVKANEVEEFLQLAKQLPFAGLSVTMPLKELILPYLDKIDPHALKIGAVNTLQMHDGQLYGHNTDIGGLALNKCNAMILGAGGSARAIAHALHKVGANIKITSRTMDRAMKLADEVKGKAIEWEARHNGECDILINCTPIGMHPNKTTLSNKKVLVIGAGGAARAICFGLKRKKAAIVIANRTIGKAEKLAEEIDCRSCFLEEMYKEDPDIIINTTSVGMYPDEDKTVVKKEFLKERVVFDLAYFPLETELIKAAKARGLKVINGLEMFIRQAAAQIQLFTNQAPDLTQMREIMRENM",
    "response#3": "MAVIGWPVAHSISPHVHNAGFEAIGFDGVYLPMPVAEGWESFKATLLSLLDARWLDLRGVSVTIPHKKNLLRLAREDRTRRWVIDPLADRAGAANTLALDGDRVIGDNTDGAGLVADLRALVLTGKKVLLLGAGGVARAIGVGAKDRGAELLVTSRTYERAVELAAKLEGEALAWEDRHSANADVLVNCTPVGMHPKMNETPFEEAALAGLPPGAWVFDAIYNPWTTELLRQAAAAGARTVSGIEMFVHQGARQFEIWTGKPAPEDEMLRVVLATLRETA"
  },
  {
    "reference": "MNAVADPHAHRLPAADIVQWLRARVPAQADLRLDSREVAAGDVFVACPGGTSDGRLYIEQAVQQGAKAVVYEAAGYDAARAAAVTAIAGAVPVLPVVELRGQLGRVADLWYGEPSAALNVIAVTGTNGKTSTVQWLARALSNSGRPCGALGTLGATLPDGQALPGHLTTPDVLAVHRQLAVMRDHGASCVAMEASSIGIEQGRVDGVRIGIAAFTNLTRDHLDYHGSMAAYEAAKSRLFDWPGLVRAVVNADDAAGQRLLARLPAELALSYGLEQGANVRASGLSATTSGQIFTLTTAQGEARIMTALLGRHNVENLLLVAGVLTALGGSLADVARELAAATPVPGRMEVVAAPQGLAAAGPLVVVDYSHTPDALARALAALRPVAQARGGRLLCLFGCGGDRDPGKRPLMGGIAAADADGVCVTSDNPRSEDADVIIDQILAGMPAARAVTVQADRAHAIMQTVWAAAAEDVLLLAGKGHETYQEIAGQRLPFDDRAWGALALALPQATGVSTDTRTLSEGQIFVALSGENFDGHNYLAQAQQAGARAAVVAHAVADTTLPQWVVGDTRAALCRIGATWRARFDLPVVGVTGSNGKTTTKEMIAAILAEWLGEDQRLATAGNYNNDIGVPLTLLRLRPQHRAAVIELGMNHPGEIAALAAMAAPTVGLVNNAQREHQEFMHSVEAVARENGAVLAALPEAGYAVYPGDDAHTGVWDAMTGTPRVLRFGLQAGLDVYAEAIERDVLSTRCRLVTPAGTAELTLPVPGL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be designed to have high stability and activity towards ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.2. For general function, the protein need meet that Involved in cell wall formation; Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.3. The protein should be optimized for stability and expression in cytoplasm where it will participate in peptidoglycan biosynthetic process. The designed protein sequence is ",
    "response#1": "MRVSGLALDSRQVRSGDAFFALRGTRGHGIEFVRGAVQRGATVVLAEAPAPQDAEDPGVPVVWIDGLHTFVGEIAARLYPDRPSQVIGVTGTNGKTTVCYLLESIFSAAGYKTGVIGTINYRYNTKLFEAIHTTPFSCDLQKILYEMVKEKIEVVIMEVSSHSLVQKRVEGCEFDIAIFTNLSSEHLDYHKTMENYFEAKSLLFKNLKEEKKEIFANSSLGNKTCVINYKDEYGKKLINLCSIKNILLYRINNKNNKNGDYPIFSAINIKYSPLSTEFEIINCLNKIKIYSNLIGKFNVENILAAFVAVTGTSGKSSVADFARQLFAALGHSSASLGTLGVITSDGAAYGSLTTPDPISLHETLDRLARGGVTRLAMEASSHGIDQRRLDGVRLSVAGFTNLGRDHLDYHGTTEAYAAAKLRLFDTLLPPGGPAVVNADGPFADVFMEGVRRAGRTLLTTGAAGTALRLVEARPDGFDQNLTVEAFGRTVRTRLPLLGGFQVENALVAAGCLWAMGHDLDDIVRVLPQLQGAVGRMNRIGGVHGLPLVVIDYAHTPDALEQALTALRAHCEAKLICVFGCGGERDAGKRPQMGEIAERVADMAIVTDDNPRGEDGDTIVAHILAGMSRPQAATVQRDREAAIRAALTMARTGDVVLIAGKGHEPYQEIAGVRHPFSDAEHARAALQERLES",
    "response#2": "MKLNELLKHIPVRLIKGDTDLAVAGVETDSRKVKRGSCFVAVAGSAADGHAFVGEAERAGAIALVVSRPVASVVVTSVEYDSRNVKRGSLFIAVEGFNSDGHRFIASAIENGASAVCVARDRARDFASLHQDGTGVLAADDTRTALSRLSAARFYGYPSQKLKLIGVAITGTNGKTSVSFMVESVLRIAEGVTGTNGKTTSVTLLHKLFRELGYHVGLLSTVQNQIDEEIIPSTHTTPDAVRLNELLAQMVKAGCTHCFMEVSSHAMVQQRVAGLTFAGGVFTNITHDHLDYHGSFDEYIKAKKSFFDMLPKSAFALINADDKRGPVMVQNTKAAVHYYALRKVVDFKARIIDNTIQGLHLEVDGQEIWCKLIGAFNAYNLLGAYGVAIGLGIPFDAIRTGLQNFPGVPGRFESVDCGQDFGVIVDYAHTPDELENVLRTAKSLTKNRLIAVFGCGGDRDRGKRPIMGGIGAELADLVVVTSDNPRSEDPDSIIEMVLEGIPDPEKSRATVRPDRREAIETAIGMAQAGDVVVIAGKGHEDYQILGTEKIHFDDREVARDALRGRKN",
    "response#3": "MELAALAERLGARLVGDPALPVTGVSTIASDSRKAVGGSVFFAVPGTRADGMSFVPQAVAAGAVAVVAEAERPAGLAPGVAFLQVPDVRRALALAASRFFGDPSARLTLVGVTGTNGKTTTARIIKEILERSGKKVGLIGTLGVFFGSTFYEPTLTTPDPLDLHKILHEMYHYGIDYVVMEVSAHAIDLKKLAFVRFEIGVLTNLTQDHLDYFGTMEKYRAAKKAFLTSPQCKTVVVNSDDELGREILLENDKVVSYGIDNPADVFAIDVKEELDGTSFILNLFDGIYDVKMKLLGLFNVYNALAAEVAIATGIEPRVVAEGVAGTGGVPGRLERVGEVDGALCIVDYAHKPDALAHVLDALRPFTKGRLVCVVGCGGDRDRGKRPLMGRIAVEKADTVIVTDDNPRSEDPAAIRAEVMKGAPGATEIGDRAEAIRAAVRGLRPGDVLVVAGKGHETSQIVGSVTLPFDDAEVVTSVEYDSRNVKRGSLFIAVEGFNSDGHRFIASAIENGASAVCVARDRARDFASLHQDGTGVLAADDTRTALSRLSAAFYLQPPRVVA"
  },
  {
    "reference": "MSVILPESHPEHPFNLIPELCRRFYDLGWATGTGGGISIKMGDNYYVAPSGVQKERIKSNEIFVLNSSQDIVEKPRTDKQLKMSECTPLFFNAYRMRNAGACLHTHSVKCVLISLLCDREFRISHIEMLKGISNNETKKALGFRDTLIVPIIENTDFEKDLTASMAECMEKYPESCAVLVRRHGMYVWSDTWQKAKGAVECIDYLMGLAIQMKQLGLEWEPKDVK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should interact with enzymes and substrates within the L-methionine biosynthesis via salvage pathway pathway to enhance or regulate its activity.2. The protein must exhibit the following characteristics: Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P)..3. The protein design must be focused on enhancing the efficiency of L-methionine salvage from methylthioadenosine. The designed protein sequence is ",
    "response#1": "GIEGVRQLIQVHDRPLLMSIFKGVIGRDLTFLREQLEAQLAGGIDLVKDDEILYDNPLTPALDRARIGRDVLNAHYEKTGKRALYAITLSGSVFGLKDQTKRLIEAGATAFLLNTFTYGLDVLRELAADPDISVPIFNHPALSGALIASPEYGIAAPVLLGTLPRLAGADLTLFPSPYGNVALAKDLARGIAVEATRIGETKTILPVPSAGIHPGLVAQLVHDFGTDSVINAGGGVHGHPQGAAAGVLAFRQALDAALAGESLTSAATRQEELRVALDAG",
    "response#2": "GIEGVRQLIQVHDRPLLMSIFKGVIGRDLTFLREQLEAQLAGGIDLVKDDEILYDNPLTPALDRARIGRDVLNAHYEKTGKRALYAITLSGSVFGLKDQTKRLIEAGATAFLLNTFTYGLDVLRELAADPDISVPIFNHPALSGALIASPEYGIAAPVLLGTLPRLAGADLTLFPSPYGNVALAKDLARGIAVEATRIGETKTILPVPSAGIHPGLVAQLVHDFGTDSVINAGGGVHGHPQGAAAGVLAFRQALDAALAGESLTSAATRQEELRVALDAAR",
    "response#3": "GIEGVRQLIQVHDRPLLMSIFKGVIGRDLTFLREQLEAQLAGGIDLVKDDEILYDNPLTPALDRARIGRDVLNAHYEKTGKRALYAITLSGSVFGLKDQTKRLIEAGATAFLLNTFTYGLDVLRELAADPDISVPIFNHPALSGALIASPEYGIAAPVLLGTLPRLAGADLTLFPSPYGNVALAKDLARGIAVEATRIGETKTILPVPSAGIHPGLVAQLVHDFGTDSVINAGGGVHGHPQGAAAGVLAFRQALDAALAGESLTSAATRQEELRVALDAV"
  },
  {
    "reference": "MSCLPEPTVLTQLWVDNPPRYSQAQLQDYLKVIKLPQRFLNSAVLKDSSLAHTKEHGLPLLKAITRYHACNVPFENLELHYSAHKTITLDPVELFEKIVTRRRGGRCMENNTFLGTVFRSFGYEVRDCGGRVARAMSPYPDVRKNQAYTYDGWNHMLNLVYLEDEWYVVDVGMGSMGPNLPFPLRHGFETMSIAPRKIRLQRRPIAESHASNPTKGTELWCYDVCLKPTKDGTEVWIPTYAFTETEFLPQDYEVMSWFTSTNPRSFFTRYVTCTKMIQDEEKEEIIGNLTLFKDTIRETIGNQRKVVRECKTEEERIQALVEIFDVNLTDEEKNGLPEERRLA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "FKGRWTACVSTQVGCAMGCVFCATGQMGFTRHLAPGEIVGQILFVNRALAENGESLRNIVFMGMGEPLHNYDHTMTAIDILTDHKGLAIAPKHITLSTVGVVPGILRLAEEERPFRLAVSLHGATDEDRQKLVPPARRWPLAELMDACRTYTKARNRHIFFEWTLIEDENDTPEQAHALGQLLQTVDSHVNLIPLNPTLGYNGRPTQRPQVKQFQAILAEYGIPSTVRQRRGIA",
    "response#2": "FKGRWTACVSTQVGCAMGCVFCATGQMGFTRHLAPGEIVGQILFVNRALAENGESLRNIVFMGMGEPLHNYDHTMTAIDILTDHKGLAIAPKHITLSTVGVVPGILRLAEEERPFRLAVSLHGATDEDRQKLVPPARRWPLAELMDACRTYTKARNRHIFFEWTLIEDENDTPEQAHALGQLLQTVDSHVNLIPLNPTLGYNGRPTQRPQVKQFQAILAEYGIPSTVRQRRGIG",
    "response#3": "FKGRWTACVSTQVGCAMGCVFCATGQMGFTRHLAPGEIVGQILFVNRALAENGESLRNIVFMGMGEPLHNYDHTMTAIDILTDHKGLAIAPKHITLSTVGVVPGILRLAEEERPFRLAVSLHGATDEDRQKLVPPARRWPLAELMDACRTYTKARNRHIFFEWTLIEDENDTPEQAHALGQLLQTVDSHVNLIPLNPTLGYNGRPTQRPQVKQFQAILAEYGIPSTVRQRRGIG"
  },
  {
    "reference": "MTKKLWFLPIVCLFFILGWTAPSASAGAPADTNLYSRLAVSTAGGTTLFPQTSSAVITPSADTETYYKEASGKSGTALKSALHRIISGHTKLSYSQVWNALKETDEDPANPNNVILLYTQESRAKSKNGGSVGDWNREHVWAKSHGNFGTAAGPGTDIHHLRPADVQVNSARGNMDFDNGGSEYPKAPGNYYDGDSWEPRDEVKGDVARMLFYMAVRYEGGDGYPDLELNDKTGNGSAPYMGKLSVLLKWNKQDPVDSKEKRRNEIIYEDYQHNRNPFIDHPEWADEIW",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Incorporate a signal peptide in the protein design.2. For general function, the protein need meet that Mg(2+)-activated ribonuclease which hydrolyzes RNA apparently nonspecifically into oligonucleotides with 5'-terminal phosphate.3. The protein can be targeted to extracellular region and perform nuclease activity. The designed protein sequence is ",
    "response#1": "MKVTIYTDGAARGNPDGPGGYGTILSYIDSTGVEHIREYSGGYKKTTNNRMELMAAIVGLEALTKPCVVTLYSDSQYVVKAFNEHWLDGWIKKGWKRGKNEPVKNVDLWKRLLAAKNQHDVTFCWVKGHDGHPQNERCDVLATTAAAE",
    "response#2": "MKVTIYTDGAARGNPDGPGGYGTILSYIDSTGVEHIREYSGGYKKTTNNRMELMAAIVGLEALTKPCVVTLYSDSQYVVKAFNEHWLDGWIKKGWKRGKNEPVKNVDLWKRLLAAKNQHDVTFCWVKGHDGHPQNERCDVLATTAAR",
    "response#3": "MTPEVVIYTDGACSGNPGPGGWGAILIHGEREKELCGGEAATTNNRMELMAAIQALEALKRPCRVELHTDSQYVQKGIHEWIHGWKKRGWLTADKKPVKNDDLWKRLDAARLRHHVDWRWVKGHAGHELNERADALARKGLV"
  },
  {
    "reference": "MNPRLRHWREAATLLLAAGTGARPGRSALGPCDYEVLLLKRSSRSGFAPGAHVFPGGLVEAADFSAAWLGLLPASPLCGLGSVKPPPAGSGRAPIFATDRRQLGSPLPGEVAFRICAIRETFEEAGILLLAPGSGPGESGGAGPLPAESLLSAAEVGEWRRRVREDAGCFLQLCRHLGCVPNIWALHEWSNWLTPVGRAGPGGRRYDTAFYLCCLGERPPHASEDEREVTACLMTSKSLATQSFVQYQQSADTIQLTLSRPREIRVWSSPPEAIELFKSREISLAPPQFYELCRLCNFSSLHELHKFSSDRALEGCECWMPIMLTASDGLIQLLPGDELYPEDPDYTGEMNTVMSTDKMVEDLMKEGSTFHRIVIKNINSLALYVNIQAKYKHMNPLMINTDCSDYNSRL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The presence of Mn(2+) is necessary for the protein to function in the desired environment.2. The protein have enhanced dodecanoyl-CoA + H2O = adenosine 3',5'-bisphosphate + 2 H(+) +   S-dodecanoyl-4'-phosphopantetheine.3. The designed protein should be optimized for magnesium ion binding, hydrolase activity activity in order to improve propionyl-CoA metabolic process, succinyl-CoA catabolic process, malonyl-CoA catabolic process. The designed protein sequence is ",
    "response#1": "GHRLGADCLSARTPPPAAPGSPLDSSYGRSHLPHKSCFDYDVLLLKRSSKSGFMPNAYVFPGGMVDSSDFSSEWLDIFKSFRTFPNFGLRSVTQPVETRPPMFATDRLKLGSPIPGEVAFRICALRETFEESGVLLVVSKVEEKSLLKSIEDRRATDQVPHNNVSDLCGSELTKWRALVNQNPSNFIRMCRELEVLPNIWALHEWGNWLTPTGRYGMTRFDTAFFICCLQEIPHTLHDEKEIVRYQWSTPSEVLQSYQARELWIAPPQFY",
    "response#2": "GIVAGKKGMTGAAYLATMAALRAGSGLVYAIVPDTISQILSIKLTEAIIRPVEDKQKGYFILDSLDEIMEVIKDMDVIAVGPGIGVNEETVQVVRNILLNYDKTIVLDADGLNCLSEMGLDILYERKGDTIITPHPGEMSRLIGVKTGEIQKKRVEYSLELSNKYNITVVLKGNGTVVVSPDKEIYINTTGNPGMATAGSGDVLTGIITSLAGQRIGTHESAVLGVYLHGLAGDLAKACKGEYGMIARDILNNIL",
    "response#3": "GHRLGADCLSARTPPPAAPGSPLDSSYGRSHLPHKSCFDYDVLLLKRSSKSGFMPNAYVFPGGMVDSSDFSSEWLDIFKSFRTFPNFGLRSVTQPVETRPPMFATDRLKLGSPIPGEVAFRICALRETFEESGVLLVVSKVEEKSLLKSIEDRRATDQVPHNNVSDLCGSELTKWRALVNQNPSNFIRMCRELEVLPNIWALHEWGNWLTPTGRYGMTRFDTAFFICCLQEIPHTLHDEKEIVRYQWSTPSEVLQSYQARELWIAPPQFY"
  },
  {
    "reference": "MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWLPVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVWSAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYEDFIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWGSTAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYGATLSAMHFVRVLDNAIGGKA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should contain one or more Fe/B12 periplasmic-binding domains that are essential for its function.2. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "RIVCCGPGCLRLIVYLNATDKVVGVEDTEKKWTPWTRPYRIAHPELANLPTIGQGGPCPKPNPEAIIQVKPDVIFVTYMPKDEVDALQQKTGIPVVVLSYGQLATFNNEDLFKSLELAGKILGKEERAKEVIEFIKNCQNDLNERTKDIPDDKKPSVYVGGIGYKGLHGIDSTECKYPPFVAVNAKNVADELGKEGHVFVTKEQILKWNPDIIFIDEGGLKLVVEDYKRNKEFYNSLKAFKNGDVYGLLPYNFYTTNIGTALADAYYIGKVVYPDAP",
    "response#2": "RIVCCGPGCLRLIVYLNATDKVVGVEDTEKKWTPWTRPYRIAHPELANLPTIGQGGPCPKPNPEAIIQVKPDVIFVTYMPKDEVDALQQKTGIPVVVLSYGQLATFNNEDLFKSLELAGKILGKEERAKEVIEFIKNCQNDLNERTKDIPDDKKPSVYVGGIGYKGLHGIDSTECKYPPFVAVNAKNVADELGKEGHVFVTKEQILKWNPDIIFIDEGGLKLVVEDYKRNKEFYNSLKAFKNGDVYGLLPYNFYTTNIGTALADAYYIGKVVYPDK",
    "response#3": "RGTDVAHVVLRFVCLLTSVIALSLMATAKEAASISIYGFLLPVSSKWSFSDSFEYLVGVSAAVAAHALLQLIISVSRLLRKSPVIPSRNHAWLIFAGDQAFAYAMLSAGSAASGVTNLNRTGIRHSPLPNFCKPLRSFCDHVAASIAFTFFSCFF"
  },
  {
    "reference": "MHDWLNNLPKAELHLHLEGSLEPELMFRLAERNKIALPWNDVEALRSAYNFGNLQEFLDLYYAGADVLRTEQDFYDLTWAYLQKCEEQNVVHTEPFFDPQTHTDRGVPFEAALNGISAALADGRELLGISSGLILSFLRHLPEEEAFKTLEQALPYRNAFFAVGLDSSEVGHPPSKFQRVFDKARGEGLLTVAHAGEEGPPEYIWQALDLLKVSRIDHGVRAAEDPKLIARLIEEQIPLTVCPLSNTKLKVFADMRQHNILDLLEQGVKVTVNSDDPAYFGGYVTENFVALHESLGMTEAQARRLAQNSLDARLAY",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Plays an important role in the purine salvage pathway and in nitrogen catabolism.2. The designed protein must possess adenine deaminase activity to support hypoxanthine salvage, nucleotide metabolic process under certain conditions.3. The design of the protein must focus on the precise arrangement of amino acids in the Proton donor to enable effective catalysis of adenine + H(+) + H2O = hypoxanthine + NH4(+). The designed protein sequence is ",
    "response#1": "LFTEKRIEQVAIQDGKFVPTVENDLLKMAVVERHHDTGCVGLGIVKGFGLTEGAIATTVAHDSHNIVAVGISDEAMKAAIDHITQTGGGIAVVNGAGQVLHDLALPIAGLLSDKSYEEVENDLAGLLNAFKQISTADGFDPFLTLSFLTLPVIPELKLTDQGLFDFATFR",
    "response#2": "LCPGLMDGHVHFESSMVTLSQFAVPALAHGTTSVVIDPHEIANVLGRGGIELVLDEAATLPLNAFVAVSSCVPATSFETAGASIDVDDIVSLIANENVVGLGEMMDYPGVVFCDETKLSMIRTALKERLVVDGHCPALSREQLFGYMCAGISTDHESIEYEEALEKLRLGMKLMIREGSAAKALDKFLPRLIGDGVSLENVFFVTDDKHPSDLLKGYMDVIVRRAIELGLSPLDAISMCTINAAKHYRVDHIVGSLSMGRKADIIVLEDLEKFIIDSVYASGRPE",
    "response#3": "PKCEHHVHLEGCVTPELIFQLAEKNNIQLPNPATHPAYASVEALRARYAEFTSLNDFLDFYFHGMSVLHHQSDFEELAWAYFQKAHADGVHHAEVFFDPQVHQARGIDYETVVSGFTAGCKRAERELGLSTRLILCFVRHLPVDSATKLYELALASNHFESRVVHGLGWSSSEVGPPKDMFREIYASASGKGIRLTAHAGEEGDPTYISTALELGARRIDHGIRLVEDPELMERVAREGIMLTVCPLSNVRLRCVQSVEQVPIRKFLDAGVKFSINSDDPAYFGGYILDNYCAVQEAFQLSILEWRVIAENSVNESWIDEARKAELLKRIDSY"
  },
  {
    "reference": "MSNQRKRSNDEREEEDDEDAEGIGEWERAYVDDRSWEELQEDESGLLRPIDNSAIYHAQYRRRLRMLSAAAAGTRIQKGLIRYLYIVIDFSRAAAEMDFRPSRMAIMAKHVEAFIREFFDQNPLSQIGLVSIKNGVAHTLTDLGGSPETHIKALMGKLEALGDSSLQNALELVHEHLNQVPSYGHREVLILYSALCTCDPGDIMETIQKCKKSKLRCSVIGLSAEMFICKHLCQETGGLYSVAVDEVHLKDLLLEHAPPPPAIAEFAIANLIKMGFPQRAAEGSMAICSCHKEVKIGAGYMCPRCKARVCDLPTECTICGLTLVSSPHLARSYHHLFPIAPFDEVPALSSLNDNRRKLGKSCFGCQQSLIGAGNKPVPCVTCRKCKHYFCLDCDIYIHESLHNCPGCESIHRPKSVSLMEE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription ; When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape; Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II; Can restore UV resistance in the NER-deficient ssl1-1 yeast mutant.; In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment2. The protein should be optimized for stability and solubility when expressed in transcription factor TFIIH core complex where it will be involved in regulation of transcription by RNA polymerase II, nucleotide-excision repair, DNA-templated transcription.3. The presence of VWFA domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "CPCNLTAGICDIRCCCDLECTPDLKQLFSGSCFAGVFGGNVNLPFDQLCSVQTTNSAPDWFPFLCVQSPLNNSPFLGYFYHGSVSSSLVSSFKIPLQTVPEKPLSGYKQGDPIMTTENEYFTIRQRSMAGQCVGNAPVAFLQNFDVKCVTSLSAYKEGLSHNVMINSGTGDTIQQNVIYGSVTDVRNFITKPEDIQRTEVLCINVTFAEYYMFIWKGKTIEEINVTVLIEELRKNKFKKQIFDDLGIEKEIDIRKRVSGIYNKTEEDFDDLKDYNKYLESVENIIFNLNNGIDIEETESNLVKYENEHKIEILEKNMRESQKNSDLAKYQEAMARLKQEKVKIQKQMEIEDLEFQKQQKQELLDKLSTAQSANSEELITQSNNNILKRSSLRKRQLQQLTSQLDQQFQSSNPFSKQAKAIEDEDNRTPFTPVREWSIKLVCLNPQGEETDASFVDRVTYKLHPTFQNPTRTIRKPPFQIKEQGWGEFEMEIIIYYADKGGEHRFLHYLHFQSPHAQPRPYQEKSLSKMFGNGRARSGIIVLPCGAGKSLVGVSAAARIKKSCLCLATNAVSVDQWAFQFKLWSTIRDDQICRFTSDSKERFRGNAGVVVTTYNMVAFGGKRSEESEKIIEEMRNREWGLLLMDEVHVVPAHMFRKVISITKSHCKLGLTATLVREDERITDLNFLIGPKLYEAPSTQIRPYQEKSLSKMFGNGRARSGIIVLPCGAGKTLVGITAACTIKKSVIVLCTSSVSVMQWRQQFLQWCTLQPENCAVFTSDNKEMFQTESGLVVSTYSMVANTRNRSHDSQKVMDFLTGREWGFIILDEVHVVPAAMFRRVVSTIAAHAKLGLTATLVREDDKIGDLNFLIGPKLYEA",
    "response#2": "CPCNLTAGICDIRCCCDLECTPDLKQLFSGSCFAGVFGGNVNLPFDQLCSVQTTNSAPDWFPFLCVQSPLNNSPFLGYFYHGSVSSSLVSSFKIPLQTVPEKPLSGYKQGDPIMTTENEYFTIRQRSMAGQCVGNAPVAFLQNFDVKCVTSLSAYKEGLSHNVMINSGTGDTIQQNVIYGSVTDVRNFITKPEDIQRTEVLCINVTFAEYYMFIWKGKTIEEINVTVLIEEE",
    "response#3": "VIDCSRFMTSKAMPPSRFVVVMKALQTFLDRFFEQNPIAQIGLITCKDRKADRLTMMTGNIRVLKESLNTLTEAFCGGDFSLQNALQLACANLKGMPGHVSREVVLVISALSTIDPGNIYSTIETMKRMNIRCSAIGLSAEMFVCKEMAKATKGEYSVALDPDHLQLLFSKHTLPPSSAKSSECNAIHVGFPVREWSIKLVCLNPQGEETDASFVDRVTYKLHPTFQNPTRTIRKPPFQIKEQGWGEFEMEIIIYYADKGGEHRFLHYLHFQDTLDCSEAMLEKDLRPNRHAMIIQYAIDFVHEFFDQNPISQMGIIIMRNGLAQLVSQVSGNPQDHIDALKSIRKQEPKGNPSLQNALEMARGLLLPVPAHCTREVLIVFGSLSTTDPGDIHQTIDSLVSEKIRVKVLGLSAQVAICKELCKATNYGDESFYKILLDETHLKELFNEAVTPLPVNKINKGFTLVKMGFNYVHGIIRSLILTLDCSEAMLEKDLRPNRHAMIIQYAIDFVHEFFDQNPISQMGIIIMRNGLAQLVSQVSGNPQDHIDALKSIRKQEPKGNPSLQNALEMARGLLLPVPAHCTREVLIVFGSLSTTDPGDIHQTIDSLVSEKIRVKVLGLSAQVAICKELCKATNYGDESFYKILLDETHLKELFNEAVTPLSESKFCYACETRLSGEGFRCGSCQLVFCLDCDTLLHESLHVCPGCHRPTTLDCSEAMLEKDLRPNRHAMIIQYAIDFVHEFFDQNPISQMGIIIMRNGLAQLVSQVSGNPQDHIDALKSIRKQEPKGNPSLQNALEMARGLLLPVPAHCTREVLIVFGSLSTTDPGDIHQTIDSLVSEKIRVKVLGLSAQVAICKELCKATNYGDESFYKILLDETHLKELFNEAVTPLPVNKINKGFTLVKMGF"
  },
  {
    "reference": "MVQSKAKAGFQAGVKDYRLTYYTPDYTPKDTDLLACFRVTPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTDLDRYKGRCYDIEPVPNEDNQYFCFVAYPLDLFEEGSVTNILTSIVGNVFGFKALRALRLEDIRFPVALLKTFQGPPHGITVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENINSQPFMRWRDRFLFVQEAIEKSQAETNEIKGHYLNVTAGTLEEMMKRAEFAKEIKTPIIMHDYLTGGFTANTTLARWCRDNGVLLHIHRAMHAVIDRQKNHGIHFRVLAKCLRMSGGDHLHSGTVVGKLEGERGITMGFVDLMREDYVEEDRARGIFFTQDWASMPGVMPVASGGIHVWHMPALVEIFGDDSCLQFGGGTLGHPWGNAPGATANRVALEACIQARNEGRSLAREGNEVIREAARWSPELAAACELWKEIKFEFEAMDTL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein with ribulose-bisphosphate carboxylase activity is needed to enhance photorespiration, reductive pentose-phosphate cycle in a specific context.2. The protein must be engineered to have a cooperative binding mechanism with Mg(2+) to ensure proper regulation.3. The protein should have a low Km value for D-ribulose 1,5-bisphosphate + O2 = (2R)-3-phosphoglycerate +   2-phosphoglycolate + 2 H(+). The designed protein sequence is ",
    "response#1": "FETLSYLPPLTDEQLLKEVEYLLRKGWVPCVEFELEKGFVHRQYNSSPGYYDGRYWTMWRLPLFGTTDAAQVLKEVAECKAEYPEAFIRIIGFDNVRQVQCISFIASTG",
    "response#2": "KKFETLSYLPPLTDEQLLKEVEYLLRKGWVPCVEFELEKGFVHRQYNSSPGYYDGRYWTMWRLPLFGTTDAAQVLKEVAECKAEYPEAFIRIIGFDNVRQVQCISFIASTC",
    "response#3": "ETFGFLPPMTQDEIYDQIAYIIAQGWSPLVEHVHPSNSMATYWSYWKLPFFGEKDLNVVVSELEACHRAYPDHHVRIVGYDAYTQSQGACFVVK"
  },
  {
    "reference": "MKIKVGILGASGYAGNELVRILLNHPKVEISYLGSSSSVGQNYQDLYPNTPLNLCFENKNLDELELDLLFLATPHEFSAKLLNENLLKKMKIIDLSADFRLKNPKDYELWYKFTHPNQELLQNAVYGLCELYKEEIKKASLVANPGCYTTCSILSLYPLFKEKIIDFNSVIIDAKSGVSGAGRSAKVENLFCEVNENIKAYGLASHRHTPEIEEHLSYAAKEKITLQFTPHLVPMQRGILISAYANLKEDLQEQDIRDIYTKYYQNNKFIRLLPPQSLPQTRWVKSSNFADINFSVDQRTKRVIVLGAIDNLIKGAAGQAVQNMNLMFDFDEDEGLKFFANL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must possess protein dimerization activity to support arginine biosynthetic process under certain conditions.2. The protein should be optimized for activity in Amino-acid biosynthesis, L-arginine biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "FSTDANLLHTSSEGKVLEDPWEEVPDYVYSRTVNPEDAPDQPEIITIDFERGDGVALNGEGMSPATLLTALNELGRKHGIGRLDLVENRFVGMKSRGMYETPGGAIYHLAHRGIEQITLDRGAAHLKDELAPRYAELIYNGFWFSPEREMLQAAIDHSQEKVTGTVRLKLYKGGVHVIGRKSPYTLYSEKVVTFEDDAGAYDQRDAAGFIKLNALRLA",
    "response#2": "FSTDANLLHTSSEGKVLEDPWEEVPDYVYSRTVNPEDAPDQPEIITIDFERGDGVALNGEGMSPATLLTALNELGRKHGIGRLDLVENRFVGMKSRGMYETPGGAIYHLAHRGIEQITLDRGAAHLKDELAPRYAELIYNGFWFSPEREMLQAAIDHSQEKVTGTVRLKLYKGGVHVIGRKSPYTLYSEKVVTFEDDAGAYDQRDAAGFIKLNALRLS",
    "response#3": "FSTDANLLHTSSEGKVLEDPWEEVPDYVYSRTVNPEDAPDQPEIITIDFERGDGVALNGEGMSPATLLTALNELGRKHGIGRLDLVENRFVGMKSRGMYETPGGAIYHLAHRGIEQITLDRGAAHLKDELAPRYAELIYNGFWFSPEREMLQAAIDHSQEKVTGTVRLKLYKGGVHVIGRKSPYTLYSEKVVTFEDDAGAYDQRDAAGFIKLNALRLR"
  },
  {
    "reference": "MNRPHLGDLDWNELEAVIKGLGQPSFRAKQVFQWVQQKGVTQWDEMTNVPKSLKEALAQHYTLAGMTVIKRDESKLDETVKYLLELRDGETIETVLMAYEQQLGRERRTVCVSTQVGCRLGCAFCATGKSGFKRNLEVNEILGQVLAVNKDLSKWSSASVTNLVFMGMGEPLLNYDNLVKSLVILNHPDGLNISMRRISVSTSGIVPGIYRLAQEEMPLVLAISLHAPNNELRNRLMPINRRYPLEELLEACHYWAERTNRRITFEYILLDRVNDNLKQAQELVDLLRGLLANVNLIPFNSVADAGFSQPSRNRVMAFRRYLEEHGVAAVIREEKGSDIAAACGQLRRQEMRGKGD",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a binding site that is specific to the target [4Fe-4S] cluster, S-adenosyl-L-methionine ligand.2. The presence of [4Fe-4S] cluster is crucial for the protein to function optimally under specific conditions.3. The protein must exhibit the following characteristics: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs..4. The protein have enhanced adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.5. The protein should have metal ion binding, tRNA binding, rRNA (adenine-C2-)-methyltransferase activity and be active in cytoplasm. The designed protein sequence is ",
    "response#1": "ISTQAGCALGCTFCATGQQGFRRNLTSGEIIEQVLQLQKIALEEDFMEVQQGIRKKGEIQGITNVVYMGMGEPLANYKNTIMSLRNIIDNTGINIGSRHITISTVGLVPQIKKLSHEKLQINLAISLHAPDNKTRSETMPVNKRFPIEELIDVCHYYVQKTNRRIFFEYVLLQGQNDKIDGLTKNGTLCVSSQVGCTLSCTFCHTGTQRLVRNLTTEEIVAQLMVARDRLGDFPGRTPPEGAFVPNDGGRFVSNIVFMGMGEPLYNFDGVRDAIDVISDNEGLSLGRRRITVSTSGVVPQIEALGAEMGTMLAISLHAVRDDLRNELVPLNKKYPIRDLLEACRNYPGLSNAKRITFEYVMLKGVNDSDAEARELVRLLKGIPAKINLIPFNPWPGTRYECSDWDRIERFSECGIMTQKQR",
    "response#2": "EKTRGTLCISSQVGCALNCSFCSTGKEGFNRNLSLAEIIGQLFLAKAKLQHTDYQITNVVMMGMGEPLLNYQPVIGAMELMMHDLAYGLSKYRVTLSTSGVVPMMQQLKKDSVASLAVSLHAPTNALREQLVPINKKYPLEVLMPVCRDYFPPDSKRCIVFEYVMLKDINDSLTHAKQLIRLLDNMSCKVNLIPFNPFPGTNYVCSDMATILVFQDRLTKAGVKTWVRKTRGDDISAAVNRERGEYENRESVCVSTQAGCAMGCAFCATGQGGLRRNLSAGEIVGQVLAFARLLAPQSARVTNVVFMGMGEPFANYDAVWKAASTLMHPAGLGLGARHIVISTVGLIPGIRRFAQERSQVRLAVSLHAPDDALRDRLVPINRAYPIGPLMEAVRDYVNLTGRRVTFEYALIAGVNDHPEQAQALAALLRGLPAHVNLIPLNPVPDSPWRPSPRARVRQFQATLAERGVPCTVRLGRGTDIDAACGQLRSRHEKDAS",
    "response#3": "EKTRGTLCISSQVGCALNCSFCSTGKEGFNRNLSLAEIIGQLFLAKAKLQHTDYQITNVVMMGMGEPLLNYQPVIGAMELMMHDLAYGLSKYRVTLSTSGVVPMMQQLKKDSVASLAVSLHAPTNALREQLVPINKKYPLEVLMPVCRDYFPPDSKRCIVFEYVMLKDINDSLTHAKQLIRLLDNMSCKVNLIPFNPFPGTNYVCSDMATILVFQDRLTKAGVKTWVRKTRGDDIDAACGQLRGRIVDRTKRLAKPV"
  },
  {
    "reference": "HDVYGGDYRITGDDGHS",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein requires pyridoxal 5'-phosphate to activate a critical catalytic site.2. The protein should be optimized for activity in L-homocysteine from L-cystathionine: step 1/1, Amino-acid biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. The designed protein should be involved in methionine biosynthetic process by localizing to cytoplasm and exhibiting cystathionine beta-lyase activity, cysteine-S-conjugate beta-lyase activity. The designed protein sequence is ",
    "response#1": "LRKIGNTPMVRINKIGKNAGLKCELLAKCEFFNAGGSVKDRISLRMIEDAERDGALKPGDTIIEPTSGNTGIGLALAAAVKGYRCIIVMPEKMSMEKVDVLRALGADIVRTPTNARFDSPESHVGVAWRLKNEIPNSHILDQYRNASNPLTHYDSTAEEILQQCDGKLDMLVASAGTGGTITGIARKLKEKCPGCTIIGVDPEGSILAEPEELNRTEVTGYEVEGIGYDFIPTVLDRKVVDRWIKSNDTESFTFCRMLMAQEGLLCGGSSGSAMAAAVKAARELQEGQRCVVILPDVVIHSATKYIDGQY",
    "response#2": "LRKIGNTPMVRINKIGKNAGLKCELLAKCEFFNAGGSVKDRISLRMIEDAERDGALKPGDTIIEPTSGNTGIGLALAAAVKGYRCIIVMPEKMSMEKVDVLRALGADIVRTPTNARFDSPESHVGVAWRLKNEIPNSHILDQYRNASNPLTHYDSTAEEILQQCDGKLDMLVASAGTGGTITGIARKLKEKCPGCTIIGVDPEGSILAEPEELNRTEVTGYEVEGIGYDFIPTVLDRKVVDRWIKSNDTESFTFCRMLMAQEGLLCGGSSGSAMAAAVKAARELQEGQRCVVILPF",
    "response#3": "LKKIGDTPMVRINKIGRNFGLKCELLAKCEFFNAGGSVKDRISLRMIEDAEREGTIKPGDTIIEPTSGNTGIGLALAAAVKGYRCIIVMPEKMSTEKVDVLRALGAEIVRTPTNARFDSPESHVGVAWRLRNEIPNSHILDQYRNASNPLAHYDITAEEILQQCDGRLDMLVASAGTGGTITGVARKLKEKCPGCKIVGVDPEGSILAEPEELNQTEQTAYEVEGIGYDFIPTVLDRTVVDKWFKSNDEEAFAFARMLIAQEGLLCGGSSGSAMSVAVKAAQELQEGQRCVVILPDVVIHSATKYIDGQDYMNTVRPLRKIGNTPMVRINKIGKNAGLKCELLAKCEFFNAGGSVKDRISLRMIEDAERDGALKPGDTIIEPTSGNTGIGLALAAAVKGYRCIIVMPEKMSMEKVDVLRALGADIVRTPTNARFDSPESHVGVAWRLKNEIPNSHILDQYRNASNPLTHYDSTAEEILQQCDGKLDMLVASAGTGGTITGIARKLKEKCPGCTIIGVDPEGSILAEPEELNRTEVTGYEVEGIGYDFIPTVLDRKVVDRWIKSNDTESFTFCRMLMAQEGLLCGGSSGSAMAAAVKAARELQEGQRCVVILPYNSDFYTTER"
  },
  {
    "reference": "MTARVILGVGGGIAAYKACELLRLFTEGGYDVRVVPTAAALRFVGAPTWAALSGHPVHDDVFADAHDVPHVALGRSADLVVVAPATADLLARAAHGLADDLLTNTLLTARCPVVLAPAMHTEMWEHPATVRNVATLRGRGVDVIEPAVGRLTGADTGKGRLPDPSEILARAVGHLRTAGDLAGRRVVVTAGGTREPLDPVRYLGNRSSGKQGWAFAEAALARGAAVTLIAANVTLPPPPGAELVAVGTTEQLRKAAVEASADADVVVMAAAPADFRPATVATDKIKKADVGDTPTIALVATPDIAAELGARKRPGQLLVAFAAETRDAVPHARRKLAAKRADLIVVNEVGVDRGFGADVNAATVLDADGVVADFPERSKRELAEAVWGVICARLPNAGLTG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should have a fast turnover rate for H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.2. The protein should be optimized for activity in coenzyme A biosynthesis, Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway and exhibit minimal cross-reactivity with other metabolic pathways.3. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.4. The CTP binding site should be located in a region of the protein that is accessible to the ligand.5. The designed protein should have phosphopantothenoylcysteine decarboxylase activity, FMN binding, phosphopantothenate--cysteine ligase activity to facilitate pantothenate catabolic process, coenzyme A biosynthetic process.6. The designed protein should contain one or more Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains that are essential for its function.7. Mg(2+) is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "MNIVVGITGGIAAYKAVNVVRALVLAGHDVHVVATENALRFVGRPTLEALSRNLVHSDLYEGVAEVRHVAIGQAADLIVVAPATANTIAKLAGGFADDLLGNTILASTAPLVIAPAMHTEMWQNPATVHNIELLRSRGVTVVGPAVGQLTGKDAGPGRMSEPDDIVAAALAAALAGGPDDLGLAGRRLVVTAGGTREPIDPVRFLGNRSSGRQGLAIASAAAHAGGSVQVIAANIESALLSALPAGVQVTRVGSALQMRDATIAAVARADALVMTAAVADFRPEVASVSKMKKDPTNEDVPTIRLIRNPDILAEIGHSEQRPPLVVGFAAETGTDEEILAYGADKAARKGADFICLNRVGESVGFGDVPNDIRVLGADGSRTEVPHGPKEDLAAAVWDLVVARLDRT",
    "response#2": "MNIVVGITGGIAAYKAVNVVRALVLAGHDVHVVATENALRFVGRPTLEALSRNLVHSDLYEGVAEVRHVAIGQAADLIVVAPATANTIAKLAGGFADDLLGNTILASTAPLVIAPAMHTEMWQNPATVHNIELLRSRGVTVVGPAVGQLTGKDAGPGRMSEPDDIVAAAVVLGVAGGIACYKSVELVRLLVQHGFAVRVVMTREAAEFVTPLTFQTLSGRPVATEIFSLTQESEIGHINLADQADVFVIAPATANLIGKIAGGIADDLLTTVVMATQAPVLLAPSMNVHMYENPILQENIRKLRRVGYHVMEPADGYLACGYEGKGRLPEPEQIVEEIRRVLGSRSSDDGLQILVTAGGTREPICPVRYLGNRSSGKQGHAIAAQAAARGAQVICVTTEADQAPQHPQITVQAVDTAAQMASAVFEAAPQSDVVVMAAAVADFTPVAVAEQKIKKSTGTPEILLQPTVDILATLGAQRSPSQVLVGFAAETSDLVENAAKKLAAKGVDLMVANDVAADQVGFGHDTNAVVILTPGGTEPVEVRGSKRAVADAVWDLVVRRLST",
    "response#3": "MNIVVGITGGIAAYKAVNVVRALVLAGHDVHVVATENALRFVGRPTLEALSRNLVHSDLYEGVAEVRHVAIGQAADLIVVAPATANTIAKLAGGFADDLLGNTILASTAPLVIAPAMHTEMWQNPATVHNIELLRSRGVTVVGPAVGQLTGKDAGPGRMSEPDDIVAAALADAAAALFERGRDGLQILVTAGGTREPICPVRYLGNRSSGKQGHAIAAQAAARGAQVICVTTEADQAPQHPQITVQAVDTAAQMASAVFEAAPQSDVVVMAAAVADFTPVAVAEQKIKKSTGTPEILLQPTVDILATLGAQRSPSQVLVGFAAETSDLVENAAKKLAAKGVDLMVANDVAADQVGFGHDTNAVVIVDAAGEEVASAAGSKDAVADAVWDLVATRLG"
  },
  {
    "reference": "MGGKWSKRSGGGWSTVRERMRRTEPAAEGVGAVSRDLAQHGAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKGALDLSHFLREKGGLEGLVYSQKRQDILDLWVYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVEPEKVEEITEGENNCLLHPMNQHGIDDPEREVLVWKFDSHLAFRHAARELHPEYYKDC",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. A protein that can perform SH3 domain binding in membrane, host cell Golgi membrane to support suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II.2. The protein must exhibit the following characteristics: Plays a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. The designed protein sequence is ",
    "response#1": "GSSPRF",
    "response#2": "ECFLPR",
    "response#3": "GLPW"
  },
  {
    "reference": "MAKSKGARIVVTLECRSAEGVYRYTTTKNRRNNPNKMELKKYCPLSKKHEVFKEIK",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein localizes to the ribonucleoprotein complex, ribosome. The designed protein sequence is ",
    "response#1": "DFPIEMQLIAMESCQVRHTALEAARVSVNRRMTEAAGLDNFYLKVVPYPHHVLREHKMATGAGADRISSGMRAAFGRPVGTAARVYQNDVIMIGRTDEAHAHELKIALKKAAIKLPTPCKVI",
    "response#2": "DFPIEMQLIAMESCQVRHTALEAARVSVNRRMTEAAGLDNFYLKVVPYPHHVLREHKMATGAGADRISSGMRAAFGRPVGTAARVYQNDVIMIGRTDEAHAHELKIALKKAAIKLPTPCKVL",
    "response#3": "DFPIEMQLIAMESCQVRHTALEAARVSVNRRMTEAAGLDNFYLKVVPYPHHVLREHKMATGAGADRISSGMRAAFGRPVGTAARVYQNDVIMIGRTDEAHAHELKIALKKAAIKLPTPCKVI"
  },
  {
    "reference": "NLYQFGNMIKCTIPKRLSWRSFVDYGCYCGKGGSGTPV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The desired protein should be able to perform a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-   glycero-3-phosphocholine + a fatty acid + H(+)2. The protein design should incorporate a binding pocket that specifically recognizes Ca(2+) to enhance enzymatic efficiency.3. For general function, the protein need meet that Snake venom phospholipase A2 (PLA2) that inhibits neuromuscular transmission by blocking acetylcholine release from the nerve termini; PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.4. The protein should be able to bind Ca(2+) ligand in a variety of conditions5. The designed protein should have calcium ion binding, toxin activity, phospholipase A2 activity within extracellular region to regulate lipid catabolic process, phospholipid metabolic process, arachidonic acid secretion. The designed protein sequence is ",
    "response#1": "LMQFENMIKKVTGRSGIWWYGSYGCYCGKGGQGLPQDASDRCCFVHDCCYGKVNGCDPKDDFYVYSSENRDIVCGEDNPCTKEICECDKAAAICFRDNMDTYQNKCLGK",
    "response#2": "NLIQFSQLIQCANKGKRATYHYMDYGCYCSKGGSGTPVDALDRCCKTHDDCYGQAEKKGCFPLLSLYNFACFPGAPQCGKGNTCQRFVCACDLKAALCFAKSPYNNNYNIDIKKKCL",
    "response#3": "DLMQFETLIMKSGVWYYGSYGCYCGSGGQFRPQDASDRCCFVHDCCYGKNGDIVCGGDDPCKKQICECDRVAATCFRDNKVTYDNKYWFFPAKCLAEEPS"
  },
  {
    "reference": "MDPVDPKLEPWNHPGSQPKTPCNKCYCKKCCYHCQLCFITKGLGISYGRKKRRQRRRTSSSGQAHQIPIPKQPSSQLRGDPTGPKE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Incorporating a Nuclear localization signal, RNA-binding (TAR), and protein transduction motif is critical for the successful design of the protein.2. For general function, the protein need meet that Tat can reactivate a latently infected cell by penetrating in it and transactivating its LTR promoter; Besides its effect on RNA Pol II processivity, Tat induces chromatin remodeling of proviral genes by recruiting the histone acetyltransferases (HATs) CREBBP, EP300 and PCAF to the chromatin; To ensure maximal activation of the LTR, Tat mediates nuclear translocation of NF-kappa-B by interacting with host RELA; In the absence of Tat, the RNA Pol II generates short or non-processive transcripts that terminate at approximately 60 bp from the initiation site; Other factors such as HTATSF1/Tat-SF1, SUPT5H/SPT5, and HTATIP2 are also important for Tat's function; Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication3. The Zn(2+) binding site should be located in a region of the protein that is accessible to the ligand.4. A protein that contributes to positive regulation of transcription elongation by RNA polymerase II, suppression by virus of host type I interferon-mediated signaling pathway by functioning in extracellular region, host cell cytoplasm with trans-activation response element binding, actinin binding. The designed protein sequence is ",
    "response#1": "MEPVDPKLEPWNHPGSQPTTACNKCYCKRCCWHCQVCFLRKGLGISYGRKKRGRRRGTPQGSKDHQNPVPKQPLPITRGNPTGPKESKKEVASKTETDPCD",
    "response#2": "MDPVDPNLEPWNHPGSQPTTACNKCYCKVCCWHCQLCFLKKGLGISYGRKKRKHRRGTPQSSKDHQNPVPEQPLPTSRGDPTGPKESKKKVASKTETDQCA",
    "response#3": "MENRWQVMIVWQVDRMRIKTWHSLVKHHMYISKKARGWLYRHHYESRHPKVSSEVHIPLGDAKLVIITYWGLHTGERDWHLGQGVSIEWRKRNYRTQVDPDLADQLIHLHYFNCFSDSAIRKAILGQIVRPRCEYQAGHNKVGSLQYLALTALIKPKRIKPPLPSVRKLTEDRWNKPQRTKGHRGSHTMNGH"
  },
  {
    "reference": "MAAWEFALGLLTASLWRWARKWRSPVKVKPMLAAVSSLEPQLEQITTDLRDRDRLLEDLPVSFLLLDADNLVLEANRSARVLLALPPEDYCRPLLEVVRSYELDRLVARCRAANAPQTDRWTLTPVNPDPLQVVPQTPRPVQGQAIPLSNGQIGVLIEDRQELVDLAQQRNRWVSDVAHELKTPLTSIRLLAEALRDRLQDEPQVWVDRLLGETQRLGQLVQDLLELSRLEQGPSGLQKLEAVDLVALLTSVRNSLEPLAEPLRLGWAYQGPEQGFVRGDRQRLFRLWLNLVDNAIRHSPSGGCLYVELRQRGDTWICDLYDDGPGFADADLPYLFERFYRGDPSRVRPAAASSSSPGSGLGLAIARQVVEAHQGRISARNHPVTGGAWLRVQLPQEPSLTPALKIGTGRRSG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Member of the two-component regulatory system SphR/SphS; SphS functions as a protein kinase that phosphorylates SphR.2. The protein should be designed to have high stability and activity towards ATP + protein L-histidine = ADP + protein N-phospho-L-   histidine..3. The presence of Histidine kinase domains is critical for the interaction of the designed protein with its intended targets.4. The protein should be designed to localize specifically to cytoplasm in order to perform its role in regulation of DNA-templated transcription more effectively. The designed protein sequence is ",
    "response#1": "TVSHEIRTPMNGVLGMLDMLLDTELKSTQRDYAQTAQVCGKALISLINEVLDRAKIEAGKIDLESVPFDLRSILDDVISLFSSKSREKGIELAVYVSERVPEILLGDPGRFRQIITNLVGNSIKFTERGHIFVQVHLADHSNLATEAKIEPVVNGMNGHKDEAIAIPTSGSHNTLSGFEAADSRNNWENFKLLLSYEKNEMPYESDSDKVTLVVSVEDTGIGIPLHAQGRVFTPFMQADSSTSRNYGGTGIGLSISKCLVEIMGGQINFVSRPLVGSTFTFTAVLRI",
    "response#2": "MPITPLLHESVARFAATGADITTRAEPDLFVSIDPDHLRRILTAVLDNAITHGDGEIAVTAHARDGAVDIGVRDHGPGFADHFLPVAFDRFTRADTARGGRGSGLGLAIVAALTTTHGGHANATNHPDGGAELRITLPTPNG",
    "response#3": "MPITPLLHESVARFAATGADITTRAEPDLFVSIDPDHLRRILTAVLDNAITHGDGEIAVTAHARDGAVDIGVRDHGPGFADHFLPVAFDRFTRADTARGGRGSGLGLAIVAALTTTHGGHANATNHPDGGAELRITLPTPS"
  },
  {
    "reference": "MEKIKKFLIQLLNMRARNTVDIIASIVGAVIIAAAISALLFSIASFLGFYSKDLSPLVYSIVSLAVIPVLRRNVPKKPILSLLTAFSIPILFLSGVEWLKLVASVLLGYLAASSLKDS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should contain a Helical membrane-spanning region. The designed protein sequence is ",
    "response#1": "MK",
    "response#2": "AXGXRX",
    "response#3": "MPIGVPKVPFRIPG"
  },
  {
    "reference": "MIPPAGSTPPGEALIPSVAPQDFWRSPISGYSGSVTRHISHRANNFKRHPKRRKYIRPSPPPPPNTPCPIELVDFEDLHPQRSFWELLFNGCILFGIEFSYAMETAYVTPVLLQMGLPDQLYSLVWFISPILGFLLQPLLGAWSDRCTSRFGRRRPFILVLAIGALLGLSLLLNGRDIGMALADTATNHKWGILLTVCGVVLMDFSADSADNPSHAYMMDVCGPVDQDRGLNIHALMAGLGGGFGYVVGGIHWDKTSFGRALGGQLRVIYIFTAITLSVTTVFTLVSIPERPLRPLGEKRTAMKSPSLPLPPSPPVLLEEGAGDTLPSTTATSLYASFSSPISPPSPLTPKYGSFISRDSSLTGINEFASSFGTSNIDSVLIDCFTAGHDNYLALPSSVPRQAISVSFPRAPDGFYCQERGLERREGPLTLGLDGDVLRVGSLDTSKPRASGILKRPQTLALPDVAGGNGPETSRRRNVTFSQQVANILLNGVKYESELTGSSEQSEQPLSLRRLCSTIYNMPRPVRNLCVNHFLGWLSFEGMLLFYTDFMGEVVFQGDPKAPHASEAYQKYNSGVTMGCWGMCIYAFSAAFYSAILEKLEECLSVRTLYFIAYLLFGLGTGLATLSRNLYVVLSLCTHYGILFSTLCTLPYSLLCDYYQSKKFAGSSADGTRRGMGVDISLLSCQYFLAQILVSLVLGPLTSAVGSANGVMYFASLVSFLGCLYSSLCVTYEIPSADAADEERQPLLLNV",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The ideal protein would be one that plays a critical role in glucose transmembrane transport through galactose:proton symporter activity, glucose:proton symporter activity, glucose transmembrane transporter activity within membrane.2. The protein must exhibit the following characteristics: Proton-associated glucose transporter in the brain..3. The protein should contain a Helical membrane-spanning region.4. The protein should be designed to have high stability and activity towards D-glucose(out) + H(+)(out) = D-glucose(in) + H(+)(in). The designed protein sequence is ",
    "response#1": "MLLFVSMAGWMFGADTGSIGGITNMRDFQSRFADRYNPVTDTYSYSSARQGLITGMVNVGSFFGCLISSPTTDRIGKRNSIIGFCVIYLIGVIIQVTAVPSWVQIMVAKIWTGLGIGALSVLAPGYQSEVAPPHIRGTIVVTYQLCVTAGIFIAACINMGTHKLYKTAQWRVSIGINLLWGIITLVGVSFLSESPRYLVSIGKDDEAIQVMCKNAELPPDSDIIQTEYKQIKSDCEAAVEGGPCTWPEIFGKEIRYRTFLGMAIMSFNQLTGCNYYFYYGTQVFKGTGMESPYLASLILDAVNFGCTFGGLFVLEYCGRRVPLIIGGVWQSICFFIYAAVGNRALTRKNGTSNHRAGAVMIVFSCLFIFSFAQTWGPAAYVIVGESYPIRYRSKCAAVATTSNWLWNFLISFFTPFITNSIGFKYGYIFASCNLCASIVTFLFAHESKILQSS",
    "response#2": "MLLFVSMAGWMFGADTGSIGGITNMRDFQSRFADRYNPVTDTYSYSSARQGLITGMVNVGSFFGCLISSPTTDRIGKRNSIIGFCVIYLIGVIIQVTAVPSWVQIMVAKIWTGLGIGALSVLAPGYQSEVAPPHIRGTIVVTYQLCVTAGIFIAACINMGTHKLYKTAQWRVSIGINLLWGIITLVGVSFLSESPRYLVSIGKDDEAIQVMCKNAELPPDSDIIQTEYKQIKSDCEAAVEGGPCTWPEIFGKEIRYRTFLGMAIMSFNQLTGCNYYFYYGTQVFKGTGMESPYLASLILDAVNFGCTFGGLFVLEYCGRRVPLIIGGVWQSICFFIYAAVGNRALTRKNGTSNHRAGAVMIVFSCLFIFSFAQTWGPAAYVIVGESYPIRYRSKCAAVATTSNWLWNFLISFFTPFITNSIGFKYGYIFASCNLCASIVTFLFAHESE",
    "response#3": "MLLFVSMAGWMFGADTGSIGGITNMRDFQSRFADRYNPVTDTYSYSSARQGLITGMVNVGSFFGCLISSPTTDRIGKRNSIIGFCVIYLIGVIIQVTAVPSWVQIMVAKIWTGLGIGALSVLAPGYQSEVAPPHIRGTIVVTYQLCVTAGIFIAACINMGTHKLYKTAQWRVSIGINLLWGIITLVGVSFLSESPRYLVSIGKDDEAIQVMCKNAELPPDSDIIQTEYKQIKSDCEAAVEGGPCTWPEIFGKEIRYRTFLGMAIMSFNQLTGCNYYFYYGTQVFKGTGMESPYLASLILDAVNFGCTFGGLFVLEYCGRRVPLIIGGVWQSICFFIYAAVGNRALTRKNGTSNHRAGAVMIVFSCLFIFSFAQTWGPAAYVIVGESYPIRYRSKCAAVATTSNWLWNFLISFFTPFITNSIGFKYGYIFASCNLCASIVTFLFAHESK"
  },
  {
    "reference": "MKENFNIAIDGPAGAGKSTIARKVARTLDFVYVDTGAMYRAAALYMLRSGLSKPAEDVAESFRQEVCSRLKDADIRLEYQNGQQVVLLNGENVNPYIRTEEVSNMASVVSAIPQVREEMVRLQQKIASYGNVVMDGRDIGTVVLPDAQIKVFLTASVEVRARRRYLENLEKRKAGKQVSSEDLDLEAIAKDIAERDYRDTHREKGPLVQASDAVLIDSSEMSISQVAEAILNLYADQKGRPRVITARTAGFCFGVKRAVDAVYNEAENGSPIYTYGPIIHNEVVVEDLRRKGVDVIHTPEELEGRKDATIVIRSHGVTKELQQKIEASCARCIDTTCTFVKRIHEIVEKESAAGKKIIIIGNHGHAEAEGHVGWSKTPATIIENEQEAECFDGDKNIPLCVVAQTTFNGNKFSELVDILKQRQYNVTVNNTICNATRERQTEAGEIASKADIMIVIGGKSSSNTAKLYEICKEVCDRTYLIQTAEDLDLHLNGSEKLIGITAGASTPNNIIEEVQKDVRRTDF",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein requires [4Fe-4S] cluster to activate a critical catalytic site.2. For general function, the protein need meet that Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.; Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)3. A protein that can perform 4 iron, 4 sulfur cluster binding, 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity, CMP kinase activity, metal ion binding, dCMP kinase activity in cytoplasm to support isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway, dimethylallyl diphosphate biosynthetic process, phosphorylation, terpenoid biosynthetic process, pyrimidine nucleotide metabolic process.4. The designed protein should have a binding site that is specific to the target ATP, [4Fe-4S] cluster, substrate ligand. The designed protein sequence is ",
    "response#1": "MTTDIKPREIVSELDRYIVGQHAAKRSLAVAVYNHYKRVQHNPEFREDVELV",
    "response#2": "MRSLQNLLYKTKVRAVAGNTATEVLDAALLETDTDSRKVTEGSIFCALPGSHTNGAEFIPDAINRGAVAIVAERAVDLSVPQFVCKNVREAMALAAADFYGHPSSELTLVGA",
    "response#3": "MAVLDREQILSGIQIADLGESRDLAKDALESACAKLDEALAQGEAGSALTLAESDVTGLRELLERRSYAYRMVLNAEQTGISFENAKKIALAAACLESVTKDLFSDEEKSFLEFLNRSAELPVADKLRGLKYRLEAAHKGLTDEGRELIQSTEKKLADFRSSPEIDAFLKSFSEDGKFSVLWPRFRQLRNRISYPDGRLAPLDPNVPVSVDLSYARDFMREVYPAAYPAFEKFIALYEEKGLLPGLSRSQKSIGCDITNLGAPDLRKLLSAYHFDPLTGETLPLPVRVATGEELPAEYRAEFAAKTEKFFKQIPDDLKERFARAKQEIGEAQPIREPKDLLALVSARLGVEKAGLLSQLKEKAAGESPVAPPPETKPVQAAPVPAPAPAKAPEKKAAPAKKPGPAAEDDSILSALPAGSALTKAEADELLGLSPDELDKLPAGAREKVAGLRREKEEKAAATPAAAPAAAPAPEKKAAPAKKPGPAAEDDSILSALPEGAALTKAEADELLGLSPDELDKLPAGAREKVAGLRREKEEKAAPAAAPAAPAAGKPAPEKKAAPAKKPGPAAEDDSILSALPEGAALTKAEADELLGLSPDELDKLPAGAREKVAGLRREKEEKAAPAAAPAPAAPPAAE"
  },
  {
    "reference": "MDPIDPNLEPWNHPGSQPKTPCNNCYCKMCCYHCQFCFTRKGLGIYYGRKKRKRRGATESSKDHQDTIPKQPLSQSGGTQTGQKESKKEVEKTTTSDQCA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein that contributes to negative regulation of peptidyl-threonine phosphorylation, positive regulation of transcription elongation by RNA polymerase II by functioning in host cell cytoplasm with actinin binding, trans-activation response element binding.2. The designed protein should have a high specificity for Zn(2+) binding, with minimal off-target effects.3. The protein design should emphasize Polar residues compositional bias.4. For general function, the protein need meet that This also contributes to the increase in proviral transcription rate, especially when the provirus integrates in transcriptionally silent region of the host genome; Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation; In the cytoplasm, Tat is thought to act as a translational activator of HIV-1 mRNAs.; Besides its effect on RNA Pol II processivity, Tat induces chromatin remodeling of proviral genes by recruiting the histone acetyltransferases (HATs) CREBBP, EP300 and PCAF to the chromatin; The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive The designed protein sequence is ",
    "response#1": "MENRWQVLIVWQVDRMRIRTWKSLVKHHMYISKKARGWFYRHHYESRHPKVSSEVHIPLGDAKLVVTTYWGLHTGEREWHLGQGVSIEWRKRRYXTQVDPDLADQLIHVYYFDCFSESAIRQAILGHIVSPRCNYQAGHNKVGSLQYLALTALIKPKKIKPPLPSVTKLTEDRWNKPQKTKGHRGNHTMNGH",
    "response#2": "MDPVDPKLEPWNHPGSQPTTACNKCYCKKCCYHCQKCFLTKGLGISYGRKKRRQRRRAPQDSQTHQVPLPKQPSSQPRGDPTGPKESKKKVERETETDPCD",
    "response#3": "MEPIDPRLEPWKHPGSQPQTACTNCYCKKCCFHCQVCFIRKGLGISYGRKKRRQRRRAPQDSQTHQASLSKQPTSQPRGDPTGPKESKKKVERETETDPDRD"
  },
  {
    "reference": "MAGRGKAIGSSAAKKATSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLAAVLEYLAAEVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSRLLGAVTIANGGVMPNIHNLLLPKKAGSSAKAAAADDE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must be able to perform protein heterodimerization activity, structural constituent of chromatin under specific conditions.2. For general function, the protein need meet that Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template The designed protein sequence is ",
    "response#1": "QGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKR",
    "response#2": "QGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKV",
    "response#3": "QGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKR"
  },
  {
    "reference": "MRKFNIAVVGATGAVGEEIFNVLSEVNFPVGEILPLASSKSAGSQIEFNGKSYKVKELTNEVWDEHEIDIAFFSAGGSVSEKFAPVAAASGAVVIDNTSHFRMQDNVPLVVPECNPGDIALYKETGIIANPNCSTIQMVQILKPLDDAFDIERVDVSTYQAASGAGKEGMEELVLQLQKFFEFKLDECEAKVFAHRLAFNVIPHIDVFLENDYTKEEMKMVKETQKILHKKMEVSATCVRVPVLRSHSEAITIHFKNAVDVEKAKEILSKAPSVVLLDNPSKKEYPMPLHATDTNDTYVGRIRKDNYRDNVLHLWCSADQIRVGAATNAVRIAQKWIELPENQ",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that is capable of catalyzing reactions within the L-threonine from L-aspartate: step 2/5, Amino-acid biosynthesis, L-threonine biosynthesis pathway with high efficiency and specificity.2. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.3. The designed protein should be optimized for NADP binding, protein dimerization activity activity in order to improve isoleucine biosynthetic process, 'de novo' L-methionine biosynthetic process, lysine biosynthetic process via diaminopimelate, threonine biosynthetic process.4. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..5. The substrate binding site should be located in a region of the protein that is accessible to the ligand.6. The designed protein must have at least one Semialdehyde dehydrogenase NAD-binding domain. The designed protein sequence is ",
    "response#1": "MTNVAVVGATGQVGGVMRTLLAERKFPVDTMRFLASARSAGTTIDWGNQRIVVEDLATADLTGIDIALFSAGGSASKEWAPSFAQAGAIVIDNSSAWRMDPQVPLVVSEVNPGAIEADALTADDKIIANPNCSTIQMVVALAPLHRAFGIKRLVIATYQSVTGTGVKAVKQMEDERLGIKGEKAYPWPIDLNCFPHGGSFAEDGYTTEELKLLHESRKILEAPELKVTATVVRIPVIGGHSEAVNIEFERPFELSDVRNMLETAPGVIVQDQPAQNIYPMPLTSHKKNEVFVGRLRRDNTVDNGLNMWIVADNLRKGAATNTVQIAERLVERGLVG",
    "response#2": "MLKGKIAIVGVSGAVGQEFLRVLDERNFPFDELVLFGSARSAGKEYTFKGKKYTVKELKHNDDFKGIDIAFVSAGGSISVEFAETITKHGTVMIDNSSAFRMDNDVPLVVPEVNGEDLHREYGIKRIVVSSYQAVSGTGKKAIDELHDQTRAILNYLDYEAKVYPHQISFNCLPHIDVFLDNGYTKEEMKMVNETRKILEDDSIGVTATTVRVPVFYGHSESVNVETKKPISAAEYRELLKSQPGVKVVDDPSKNEYPLAIDAAGQDLTLVGRIRDDESIENGINMWIVADNIRLHREYGIKRIVVSSYQAVSGTGKKAIDELHDQTRAILNYLDYEAKVYPHQISFNCLPHIDVFLDNGYTKEEMKMVNETRKILEDDSIGVTATTVRVPVFYGHSESVNVETKKPISAAEYRELLKSQPGVKVVDDPSKNEYPLAIDAAGQDLTLVGRIRDDESIENGINMWIVADNIRRRMPALNPIEKLSHIKRAHVSTYQAASGAGATAMEELVNQFKQILNKEDVTVEKFAYQLAYNVIPQVDVFTDNGYTKEEMKMYNETRKIMHSDIEVSATCVRVPVVRAHSESTWVETETPVSVEAAKEAFAKGNGVVLQDDPANKDYPMPLFVTGKDEVYVGRVRKDITNANGLSFWTVSDQVTGLKNAVPVLALVGATGAVGTVMISIINNRETVPWGEIRLIASARSAGKKIHVRGEDLTVVELTPEAFDGVDVAMFDVPDEVSARWAPIAAARGAVAVDNSGAFRMDDEVPLVVPEVNADRIGD",
    "response#3": "MSTLAVLGATGVVGRHILEILEQLEVPYESIKLLSSAKSAGSRLNFDGVEHIVEEAKPETFDDVDIVLASAGASTSKKFAPEIVKRGGLIIDNSSAFRMEEQVPLVIAGVNDDDLKXTPQGLIANPNCTTIAQVMVLQKLVGLESVRVATYQSASGAGQKGINELIEGVAAYLKGEPSPIAKVHARPLAFNVVPQIGTFTESGYTTEELKMIHEPRKILGLPQLAISATCVRVPVSRSHSEVVNVVTQAPLDVAAFRAALEAADGLLVVDNPQRGEFPTPVDAANRFETFVGRIRKDEAHPRGLVFWVVSDNLLKGAALNAVQIAELL"
  },
  {
    "reference": "MRTYSPKPGDADRQWHIIDATDVVLGRLASHTATLLRGKHKPTFAPHMDMGDYVIIVNAEKVALTGAKLEKKRAYRHSGYPGGLKSQSYAELLETNPVRAVEKAVKGMLPKNSLAAQQLSKLKVYKGAEHPHTAQQPQTFEIGQVAQ",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself; It is important during the early stages of 50S assembly..2. The ideal protein would be one that plays a critical role in translation through structural constituent of ribosome within ribonucleoprotein complex. The designed protein sequence is ",
    "response#1": "MXGVKGMLPKGPLGRAMFKKLKVYAN",
    "response#2": "MENRKIVLATRNKGKAREFEALLKGTPFEIVPLGAFPGVPEVLEDGATFEANAAKKAAAVARATGLLTLADDSGLEVDALNGAPGIYSARFAGEGATDAANNAKLLHELAGVPAEKRGARFVCVLVLARPGGEPLVVRGECHGVILEEPRGTGGFGYDPLFYLPALGKTLAELSLEEKNRVSHRGQALRQLLEEMRR",
    "response#3": "MTDLNLPWH"
  },
  {
    "reference": "MRAAEQRAIAAGTSVEALMERAGAALAEAAYCHAGKMPALILCGPGNNGGDGYVAARHLADRGVEVRIAAISEPKSEAAKWARGQWRGEVEALNSSTGPSLLLIDALFGTGLREGLEDALQKQFYRLAADSQMRIACDVPSGVDSDSGVELSSVPGFEMTVTFGALKPAHRLMPSMGKCGRVVLADIGIEASPEWHELGQPVLPALDPGGHKYSRGLVHALAGKMPGAIALSAKAAAYSGAGYVRVSTSRSIDGLPSAIVQTDTAEINDGRIGCLIVGPGMGDIPRVLTLALTSHAPKVIDADAITHLGEPERLRGQDAIITPHQGEFERLFGKLEGSKADRALEAAKRSGAVIVYKGPDTLVASPDGRLGFAPPAPAWLASAGTGDVLAGMIAAMRGRGLEAFEAASAAVWLHGRAAEIAGAQMIADDLAAAIPQALALLND",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should contain one or more YjeF N-terminal domains that are essential for its function.2. The designed protein should have a binding site that is specific to the target AMP, (6S)-NADPHX ligand.3. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate.4. For general function, the protein need meet that This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.; Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration5. The protein design must be able to perform NADPHX epimerase activity, ATP binding in order to contribute to nicotinamide nucleotide metabolic process. The designed protein sequence is ",
    "response#1": "MRNIEERIFSAGIPVAALMEKVAGKINHRLQTLFSEKSITSADKIGILVGPGHNGGDGLVVARELYFLGYNIVIYSPISKPKELTESHAKYAISLGINFSSNIKELYDCDILIDGLFGFGLEREITGKIANAINIINNLNKFVISIDLPSGIHTDTGEILGTAIRANITLCLGLWKQAFLQDTGLDYIGRAELIDFDIPRADIEYVGEGDLLLLKSPQKDSHKGQNGSVLIIGSNPDYIGAVVFAAQAALSQRMDLVYIIAPEASSNIIKQYNPEFIVRGVPGDTLTMDAYDAIMQLEKRVDSILIGSGSGTDEKTGELFNNILSSTEKPLVIDADALKLISPEMLSQNMVVTPHAHEFKQLFGMEVPEKIDERVEMLMNLSGDYGCQILLKGVTDVITSPEDYKLNRTGNQGMTKGGTGDLLAGLTLALSTKNTLFESAFLSAFILGSACDKLLEEKGLDYGIRDIL",
    "response#2": "MRVTDKNTQYNHIPTIVLMENAGSRIADYLINNYPDKKKVSIYSGTGGNGGDGFVIARHLLNHGYKVRLFMLSKPENIKNKDSQLNYQSIKIISQTDKNLKLSTITDSSQVSPDNSDIIIDAILGTGVNGKLRQPVSSVIDTINYSPAIVISVDVPSGMNPENGKVTDKCVVAHETLTLHKMKTGLQKAPESYVGKISVLDIGIPRVSEHKYDRGHAVVFSGGMSQTGAARLAALSALRGGAGLVTVFSPASALLVNAQHLTGIMLKRCEDGDLDVLLEDKRLNAFVLGPGFGIGDRAKAMALTVLKAGRRLVLDADGLTSFRDHASQLFEAARQCSHSEPQLVLTPHDGEFARLFPDLAGDLRLSKVDRARHASRRSNAIVVLKGADTVIAAPDGRAAINATGTPWLATAGTGDVLAGLVAAQLAQKRPSFEAACRAVWMHGKAAEHFGPGLISEDLL",
    "response#3": "MQVAELNSEYLGVTNFQLMEAVGAKAASEIIARMPKGKPNPKVHVFSGPGKNGGDGFSIARHLTSMGLRVKVTLVSRTAEVNDYAARQQLTAILQMKESVEFEAAHDSSQLKAIEAELIVDSLLGTGVKGPLREPLLGAVRALNKSRGFKVAVDIPSGLDTDTGEPHGDAVKADLTICLHKTKKGLLEAKQYVGTLVVLPIGIPLEAEVLIIGSNPDYIGAVVFAAQAALSQRMDLVYIIAPEASSNIIKQYNPEFIVRGVPGDTLTMDAYDAIMQLEKRVDSILIGSGSGTDEKTGELFNNILSSTEKPLVIDADALKLISPEMLSQNMVVTPHAHEFKQLFGMEVPEKIDERVEMLMNLSGDYGCQILLKGVTDVITSPEDYKLNRTGNQGMTKGGTGDLLAGLTLALSTKNTLFESAFLSAFILGSACDKLLEEKGLDYGIRDILKLPRLLSSATRLVLDADALNAIAQDANLQAQLTHRQRRGWITVLTPHPLEAARLLGTDAAQVMIDRLQAAQTIAAQFGAICVLKGSGTVIAAPNETTRINPTGNAALATAGTGDVLAGMIGSALARPVSSSGAALDRVASAVFQHGWLADHWL"
  },
  {
    "reference": "MQRKPSQKSATDKLFNHRVNEKITGVSRVRLVSDDGVAIVSFEEALRKAKEENLDLVEVSADQELHVCKIIDYGKYKFELLKKSKEAKKKQHVINVKEIKIRPRIESHDYEIKKKHAQEFLGKGDKVKVSLRFRGREMMHSDLGMKVVYRMIEDLKEHGTAERDPIQDGKQIVVIINPK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins.2. The protein must have high specificity towards ribosome binding, translation initiation factor activity. The designed protein sequence is ",
    "response#1": "RIMNYGKFIYEKEN",
    "response#2": "MEALVYGQMNEPPGARMRV",
    "response#3": "MPRVLQ"
  },
  {
    "reference": "MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQQEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVELILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADISQEALDLARENAKNQNLQIFLKKSDCFTEISEKYDIIVSNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYRRIAEDATDYLKDSGKIYLEIGYKQGQCVPELFRKHLPEKRVRTLKDQFGQNRMVVVDDGQD",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.2. A protein with protein-(glutamine-N5) methyltransferase activity is needed to enhance peptidyl-glutamine methylation in a specific context. The designed protein sequence is ",
    "response#1": "VMLHFLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQIQFLTGDALKPLINEGIKLNGLISNPPYIDEKDN",
    "response#2": "LEELRRVEKPRVLDVGTGTGALALGLKAAIPQAEVTATDLSPEALSLARENAALSGLDVKFVEGSLLAGLSGPFNLIVSNPPYD",
    "response#3": "VMLHFLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQIQFLTGDALKPLINEGIKLNGLISNPPYIDEKDR"
  },
  {
    "reference": "MDIWRPEKKYLRYINGFNVSELEDACFKFNYQFPKVGYCRVPSHAWCRNQGRFCATFTLYGKSKHYDKYFGVINGFTAFANTVEDAVNKLVFLAVDFITWRRQELNVYG",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. For general function, the protein need meet that May act as membrane-anchoring region for structural proteins during virus assembly, or play a role in membrane association of the viral polymerase during replication. The designed protein sequence is ",
    "response#1": "PETSSDRFYTLSSVSWTNTSKGWWWKLPDALKDMGVFGQNLYYHAMGRAGYIIHTQCNATKFHSGALLVVLIPEHQLAYIGAEKVNIAYDLTHPGETGHVLGRNTSRGNNNPDEDPFFNCNGTLFGNLTIFPHQIINLRTNNSSTIITPYINCQPMDNMLKHNNLTLLIVPLVRLRFGTEASPTVSITVTIAPYKSEFSC",
    "response#2": "PETSSDRFYTLSSVSWTNTSKGWWWKLPDALKDMGVFGQNLYYHAMGRAGYIIHTQCNATKFHSGALLVVLIPEHQLAYIGAEKVNIAYDLTHPGETGHVLGRNTSRGNNNPDEDPFFNCNGTLFGNLTIFPHQIINLRTNNSSTIITPYINCQPMDNMLKHNNLTLLIVPLVRLRFGTEASPTVSITVTIAPYKSEFSC",
    "response#3": "PETSSDRFYTLSSVSWTNTSKGWWWKLPDALKDMGVFGQNLYYHAMGRAGYIIHTQCNATKFHSGALLVVLIPEHQLAYIGAEKVNIAYDLTHPGETGHVLGRNTSRGNNNPDEDPFFNCNGTLFGNLTIFPHQIINLRTNNSSTIITPYINCQPMDNMLKHNNLTLLIVPLVRLRFGTEASPTVSITVTIAPYKSEFSC"
  },
  {
    "reference": "MKMEEMSLSGLDNSKLEAIAQEIYADLVEDSCLGFCFEVHRAVKCGYFFLDDTDPDSMKDFEIVDQPGLDIFGQVFNQWKSKECVCPNCSRSIAASRFAPHLEKCLGMGRNSSRIANRRIANSNNMNKSESDQEDNDDINDNDWSYGSEKKAKKRKSDKNPNSPRRSKSLKHKNGELSNSDPFKYSNSTGISYETLGPEELRSLLTTQCGVISEHTKKMCTRSLRCPQHTDEQRRTVRIYFLGPSAVLPEVESSLDNDGFDMTDSQALISRLQWDGSSDLSPSDSGSSKTSENQGWGLGTNSSESRKTKKKKSHLSLVGTASGLGSNKKKKPKPPAPPTPSIYDDIN",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that can perform zinc ion binding in SAGA complex to support regulation of RNA splicing. The designed protein sequence is ",
    "response#1": "MGTLEKKLDNLVNTILLKAENQHELLFGACQSDVKLTNTQEHILMLLSQQRLTNTDLAKALNISQAAVTKAIKSLVKQDMLAGTKDTVDARVTYFELTELAKPIASEHTHHHDETLNVYNRLLQKFSAKELEIVDKFVTVFAE",
    "response#2": "KTFVPRHPFDLTLAEVFFPKVPSAGAVDDSALTAALLKRNQDLSPTPSEQTAIGNLVTKVQAVLDNLVVAPGDLTTCQLEEVRQVGSFKKGTILTGNNVADVVVILKTLPTKEAVDALAKKVEADLKASMKTEVLTKGDQHTVQIHERGFDIANVHAKVRILIATLPQNLRKLEPEIHLDHKLMQSHLAAIRHTRWFEENAHHSSIKVLIRILKDLTRRFDAFSPLSAWMLDLIAHLAIMNNPSRQALPINLAFRRVFQLLSAGLFLPGSAGITDPTEPGHIRVHTAMTLEQQDVCCYTSQTLLRVLAHGGYKHILGLEGNTSVVREMSVWNGVCISPLTAVYEKPTDKKEGDLEEDC",
    "response#3": "KTFVPRHPFDLTLAEVFFPKVPSAGAVDDSALTAALLKRNQDLSPTPSEQTAIGNLVTKVQAVLDNLVVAPGDLTTCQLEEVRQVGSFKKGTILTGNNVADVVVILKTLPTKEAVDALAKKVEADLKASMKTEVLTKGDQHTVQIHERGFDIANVHAKVRILIATLPQNLRKLEPEIHLDHKLMQSHLAAIRHTRWFEENAHHSSIKVLIRILKDLTRRFDAFSPLSAWMLDLIAHLAIMNNPSRQALPINLAFRRVFQLLSAGLFLPGSAGITDPTEPGHIRVHTAMTLEQQDVCCYTSQTLLRVLAHGGYKHILGLEGNTSVVREMSVWNGVCISPLTAVYEKPTDKKEGDLEEDC"
  },
  {
    "reference": "MTAELQPEAGALLHTELMSLNVGPQHPSTHGVLRLVVDMDGEYVTRVEPHMGYLHTGFEKTMEHRTYQQNVTYAPRTDYLHSFSHELAYVLSVEKLLGAEVPERANVVRVILHELGRIHSHLVFVGTGLLDLGALTPFFYAFREKEACQDLFEAVCGYRMNQGYFRVGGLARDLPEGWTARVEAFLNQMERGVEEYTTLFAANPIFLDRAKGVGVIPADAALDLGLTGPNLRASGVPLDNRKDHPYCGFEDYDFNVISSPDGDSLARFNMRLLEFSESIKIIRQGLQKLRPGPIKDPNRKISLPPRHELETSMEAVIHHFKLVTEGFHPPLGEAYVPIESARGEVGYYVISDGGSMPYRVKIRAPSFVNLQALEYACVGVQFADLITILATIDPVLGDVDR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein must have high specificity towards NAD binding.2. The protein must exhibit the following characteristics: The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. The designed protein sequence is ",
    "response#1": "DMGALTAFFYGFRDREKILDIFEATTGGRLIQNYNTIGGVQADIAPDFVQKVKEFIAYLRPMLKEYHEVFTGNVIAQERLKGVGVLSREDAISFGATGGTGRASGWACDVRKRHPYAMYGKVDFKEIVHTEGDCFARYMVRMEEILESMDIIEQLIDNIPEGNYQEKMM",
    "response#2": "IEIPAEKLHEEMLKLRQGKHMDFLRSLTGMDWGEEGLGVVYHLEDTNTRENIVVSTRTTNREKPELPSVSDIWKGAEFNEREVYDYYGIRFIGHPDMRRLFLRDDWVGYPLRKDYL",
    "response#3": "IEIPAEKLHEEMLKLRQGKHMDFLRSLTGMDWGEEGLGVVYHLEDTNTRENIVVSTRTTNREKPELPSVSDIWKGAEFNEREVYDYYGIRFIGHPDMRRLFLRDDWVGYPLRKDYL"
  },
  {
    "reference": "MLTAAQRDLIKATVPLLETGGEALTSHFYRLMLSESEAVRPLFNQAHQAKGDQPRALANAVLMYAKNIDRLEALGPLVGQIVHKHVALQVLPEHYPIVGHYLLRSIREVLGPEIATDAIIDAWGAAYQMLADILIGAEESVYSATANAPGGWRGARSFRVADKVAESSEITSFHLVPADGGPVVDFQPGQYIGMRLVLDGNEVRRNYSLSGSANGSSYRISVKREPKGVVSNHLHDNVQVGDTLELFAPAGDFTLADGQRPVVLISGGVGITPTLPMLDKALADGRPVHFIHCARNGQAHAFGAYLAQKQAEHPQLRSYTCYSEPLPGDRADAEGLLSGELLEQWLPAERDLDAYFLGPKPFMAQVKQLLRQAGVPDAQSRYEFFGPAAALEA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design must be able to perform FAD binding, oxygen carrier activity, oxygen binding, heme binding in order to contribute to response to nitrosative stress.2. The designed protein should have a binding site that is specific to the target NADP(+), heme b ligand.3. The protein must exhibit the following characteristics: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds; Therefore, plays a central role in the inducible response to nitrosative stress..4. The protein with the reaction NADPH + 2 nitric oxide + 2 O2 = H(+) + NADP(+) + 2 nitrate, the Charge relay system must be optimized to ensure efficient catalysis.5. The designed protein should contain one or more Globin family profile domains that are essential for its function.6. The presence of heme b is crucial for the protein to function optimally under specific conditions. The designed protein sequence is ",
    "response#1": "MLSSSTIEIVKSTAPLIAETGPKLTAHFYDRMFAHHPELKDIFNLTHQDSGSQREALFNAIHGYAANIDNIEVLLPVVEKIAQKHTSFNITAEQYQIVGSHLLATIDELFSPGQPVLDAWGEAYGLLAQVFIDREQEFYEDESSSGGWNGWRNFRIESKQQESELITSFVLRPKDDQPVLRHQPGQYLTFWIEVPGQHPLKRNYSISSAPNDESYRISVKREPQGIASNWLHDQAAVGQMVKVAPPAGEFFLETDADTPVVLISGGVGLTPLTSMLETVAESGAPREVVFVHAAKNRAEHAFGERLSELADARENVDRVAAYDEPAEADRQDRDYDDEGYIDTEVLREAVPDPDAQFYMCGPVPFMRAIYDALTEAGVPAERVHYEFFGPAGSLEA",
    "response#2": "MLSSSTIEIVKSTAPLIAETGPKLTAHFYDRMFAHHPELKDIFNLTHQDSGSQREALFNAIHGYAANIDNIEVLLPVVEKIAQKHTSFNITAEQYQIVGSHLLATIDELFSPGQPVLDAWGEAYGLLAQVFIDRELFEEAQQQAYEGTRAFQIVSKTPQSALITAFEFAPQDGGDVADYQPGQYLGVWLKPEGFAHQEIRQYSLTRSPNAKNYRIAVKRENGGQVSEWLHDQARVGDTVYLAAPAGDFFMEVEPTSILVALISGGVGLTPMLGMLHMLNRGVRPFRIVSKQPQSDIITSFTLEPTDGQPVVDFQPGQYLAVYIKHDSFANQEIRQYSLTHAPNGKTYRIAVKREAQGIVSSYLHNTAREGDIIYLAAPHGDFFLEVEPTSILVYLPGQYVSVKERVIQKVKESDVITSFYLKPADHGLLPDFEAGQYITVKVDIPSIPYTCQRQYSLSCRPNKEFFRISVKREDGIGDSPDGVVSTYLHESIHEGDEIELSAPAGDQFLFK",
    "response#3": "MLSSSTIEIVKSTAPLIAETGPKLTAHFYDRMFAHHPELKDIFNLTHQDSGSQREALFNAIHGYAANIDNIEVLLPVVEKIAQKHTSFNITAEQYQIVGSHLLATIDELFSPGQPVLDAWGEAYGLLAQVFIDRETLYSERENQHGGWSGWREFVVRAKQPESDVITSFILEPKDGGPVADFEPGQYIGLAIQVPELGLRQIRQYSLSDAPNGKSYRISVKREAGDAGFHPGYVSSLLHDHIKEGDTVKLAPPFGNFYIQHESTPVAVLISGGVGITPLFSMASTILARQPERDVAFIHAARYGRVHPFREHVNQLSTAHSNIHCHTVYSEPNEQDRKEGYFDHAGLLTLDVFSRLLPPQDDYYICGPIPFMRMQHDALMALGVPESRIHYEFFGPKGSL"
  },
  {
    "reference": "MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEALQKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANTAPLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERFAPSVTPEEIEADIRALL",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The active site active site of the designed protein must be optimized for substrate binding and specificity.2. The protein must exhibit glutathione peroxidase activity. The designed protein sequence is ",
    "response#1": "DECQKIVRRIAFEKAISFIPSMCTAVTVVEENYDGPHLDDVITAEFVNDMIATFKKQGKLHKKYAFKILLDIYAYFKEQPTLVI",
    "response#2": "DMKERALIDMYSEGVADLGEMIMHFPLCPPAEKDAKLTLIREKTTNRYLPAFENVLKSHGQDYLVGNKLSRADIHLVELLYYVEELDPSLLANFPLLKALKARVSNIPAVKKFLQPGSQRKPPK",
    "response#3": "AVGLAVIMGAKIAGAKRIVAIDILESKYDMARKFGATDCVNPKSVPEGQSVQAWLVDKFDGGFDYTFECIGNVATMRQALEAAHKGWGVSCIIGVAAAGQEISTRPFQLVTGRTWKGSAFGGWKSIDSVPA"
  },
  {
    "reference": "MAQDTSQNVFQGKKVALGICGGIAAYKAAEVLRGLQKAGCAVRVAMTARACEFVQPLTFRALSGEHVVVDDYAPDNPDPIAHITFSQTVELLIIAPATANIIGKFANGIADDFLSSTYLASTAPVLIAPAMNTSMWEHPATKRNVERLKADGVIFIEPDDGEMACGTIGPGRLSEPERIVAAAMEILQTTSGVVVQDLAGEKLLITVGATREPLDPVRFLSNNSSGRMGFAIADAARKRGAKVSVIAGSTTAEAPAGIALIRAFSAAEMHAAVMNALPEASIFIGAAAVADYRPAQSAQQKIKKTDTEITIKLERTEDILADVAALNRDDLLVIGFAAETENVLANAREKLRRKNLDAIVANDVMREGVGFNTITNEVTVISRDGKAPIHVPLMPKTNVATIILDQVVRLRARVKPTSHQRVVNGRKKA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The presence of Flavoprotein domains is critical for the interaction of the designed protein with its intended targets.2. The designed protein must have a Proton donor that is structurally stable and resistant to denaturation.3. The protein design must be focused on enhancing the efficiency of coenzyme A biosynthetic process. The designed protein sequence is ",
    "response#1": "MRVALAVSGGIAAYKAAEVLRGLQRAGCRVRVAMTERATEFVTPLTFRALSGEHVIVDDYAPDNPDPIAHITFSQTVDLLLVAPATANIIAKFAHGIADDFVTSTYLACTAPILIAPAMNTTMWNHPATARNLRQLRADGVHVVEPDAGEMACGTIGPGRLSDPALIVAALDAQLSG",
    "response#2": "MRVALAVSGGIAAYKAAEVLRGLQRAGCRVRVAMTERATEFVTPLTFRALSGEHVIVDDYAPDNPDPIAHITFSQTVDLLLVAPATANIIAKFAHGIADDFVTSTYLACTAPILIAPAMNTTMWNHPATARNLRQLRADGVHVVEPDAGEMACGTIGPGRLSDPALIVAAVETALGARS",
    "response#3": "GIRVLITAGGTREPIDPVRYVGNRSSGRQGYAFAQYAAEQGASVTLVTSSELPVPPGVAVDKVETASEMFEAVKKHFEGSDIVVMAAAVADFAPARVADDKIKKADGVPEIRLVPTADILEYLGSNRRPEQVVVGFAAETSRLEENVAIKRSKKQVDIMVGNDVTKKGAGFGVETNSVYIL"
  },
  {
    "reference": "MTQARKLSDLGLTARGGDTPVITGLCVDSRAVAPGNLFAALPGAVIHGAHYIRAAVARGAVAILTDAEGAAIAKDDLATACLIVTQDPRQALAYAAALWFADQPDTIVAVTGTNGKTSVATFTRQIWMALDHAAINIGTTGVEGAWHAPSPHTTPEPITLHRLLSQAAAAGVTHAAMEASSHGLDQRRLDGVRLAAAGFTNFTQDHLDYHHTFEAYFDAKAGLFARVLPEDGVAVINIDDPKGEAMAAIAEDRGQDVLRVGRAAQADLRLLGQRFDATGQDLRLEWLGEAHQIRLALIGGFQAENVLLAAGLCIGAGDDPGRVFAALPQLTGVRGRMQLAATRQNGASVFVDYAHTPDALATALQALRPHVMGRIVVVFGAGGDRDTTKRPLMGAAAAEHADVLFVTDDNPRSEDPATIRAAILAACPDATEVGDRAEAILRGVDALGPGDALLIAGKGHESGQTIKGQTYPFDDAEQASIAVAALDGLI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should contain at least one Meso-diaminopimelate recognition motif motif to achieve its intended function.2. The protein must bind to Mg(2+) in order to perform its enzymatic function.3. A protein that is capable of catalyzing reactions within the Cell wall biogenesis pathway with high efficiency and specificity.4. The ideal protein would be one that plays a critical role in regulation of cell shape, cell cycle through ATP binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity within cytoplasm.5. The protein have enhanced ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.6. The designed protein must have at least one Mur ligase C-terminal domain.7. The designed protein should have a high specificity for meso-2,6-diaminoheptanedioate, ATP binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "MRLSELLNLFPNIKLINLSDSIKTNSKLVVAGDIFIAVKGYAHDGHFFIKDAILRGANKIVSERNISSVEYLPREKFIFCENSKIFLAELLKKYFIQQKNGIKIIGVTGTNGKTTIATLLYNMFRKMGHKCGLLSTVCNYIEDEAIPADHTTPDPIELNMLLGRMVEAGCEYAFMECSSHAIAQKRIGGLMFAGGIFTNLTRDHLDYHKTFENYRNAKKAFFDDLPKTAFAITNADDKNGMIMVQNTKATVKTYSETKMADFRGRIIECHFGGMYLDIDGHEVGVQFIGKFNVSNLLAVYGAVISCLGYSLEEIKNIVPSIKIPGRIERLRAPDGRMVVVDYAHTPDALKNVLETLLQIRGSKPYAIIGVIGAGGDRDRTKRPKMGRIAASLCDRLIVTSDNPRSEDPEMIIEEIISGIDPSDRNKVISIVNRAEAIRAACLMAHPGDVVLIAGKGHENYQEIKGVKYPFDDIEVAKKALKIIG",
    "response#2": "MELRDLEIAGLALDSREVRPGVLFLAIPGAHSDGRAHVAAAAEQGAAAIAYEAKDAPALPALDIPMIAIEGLAEQLSAIAGRFYRYPSEKLKLYGVTGTNGKTTSVFLLKSILEKLGYSCGVIGTIANYIGDNTLPSINTTPESYHLQKLFAQMVDSGCQAVVMEVSSQGLMLHRTQGFVFDYGIFTNLEPDHIGENEHKDFSDYMQCKSMLFKQCKTGIVNIDDEYGKRIFADSAAECKAYGFSDKADIRAENCELISSSLDYDLEGLIKGKRYSVNTPLCGRFNVYNALTAIAVCRHFGVSVENIKNALLEAAVPGRLERVDAGQDFTAVVDYAHKPDALEAVIATLRPLTEGRVIVVIGAGGDRDPGKRPMMGEIAARLADVVVVTDDNPRTEDPAAIRAAVLRGTASGAAEVVEQGDRRLAIREAVRRAAPGDVVLVAGKGHENYQDVLGVKHPFDERVLVKEILKERG",
    "response#3": "MSLRDFVADDSRKVKKGALFVAVSGDTHDGHDFIADALSQGAEVVIGEKTGMDELPENAVAIVVEDPRVALGRIAAGFFGTPAERLETYGVTGTNGKTTSVFLLKSILEKLGVTGTNGKTSVSQLIAQACDLLGQRCGIVGTLGNGFYGALAEGRHTTPDALKLQATLAELAEGGASTVAMEVSSHGLAQGRVAALAFKVAVFTNLTRDHLDFHGTMEAYGEAKAPLFAWPALGTRVLNLDDAFGRELASRASSARTLTYSLSDPAASLYCQDPRFDDHGVRARLVTAQGSGELRSQLIGRFNLSNLLAAIAGCHALGFDMDRIAAGLEAFEGAPGRLELVGDRFGAPVFVDYAHKPDALVKALEALRPYAKGRLVVVFGAGGDRDAGKRPLMGEIAARHADTVIVTDDNPRSEDPAAIRAAILEAAPGAQNIGDRAEAIRTAVAALAPGDVLLIAGKGHETYQIIGDKRLDFDDREHALKALAAR"
  },
  {
    "reference": "MYTPPLARLIEQLQRLPGVGPKTAQRLALHILKRPENEIQALASALIEAKKKVGLCNVCFHFSADPICEICRNPNRDKQTICVVADSRDVIALEKTREYKGRYHVLGGVMSPMDGIGPEQLYIQPLVRRVTQDGVKEVILAISPTVEGETTTLYIGQLLKPFTKVTRIAFGLPMGGDLEYADEVTLARALEGRRELD",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must have at least one Toprim domain.2. A protein with metal ion binding is needed to enhance DNA repair, DNA recombination in a specific context.3. The protein must exhibit the following characteristics: May play a role in DNA repair; It may act with RecF and RecO.. The designed protein sequence is ",
    "response#1": "STLCVVENIRDVMAIENTSQYRGLYHVLGGVISPMDGIGPGDLQIDSLVHRVASEQIHEVILALSTTMEGDTTNFFLFRKLEPTGVRVSVIARGIQ",
    "response#2": "GLICVVETVGDLWALERAGVHRGVYQVLGGTLSALAGLGPDDLNVRPLFERIEEGGVREVILALSATVEGATTAHWLQERLLPTGVTVTRVGHGVGTICVVENSQDLMALERSNAYHGTYHVLEGVISPLAGIGPSELRIAELLARIGRGGVDEVVIATNFTVEGEATALYLAKLLKPLGIRISRLR",
    "response#3": "QLMIFETTSDALKFEKLGIYRGKYFIIKNLIENVKNANEPKWKDKLLHYASNFEEIIIALNPTIEGQITSNYIKVILEEVALKVTKLK"
  },
  {
    "reference": "MSKVLIVAKGMGLKSTPSKLNLVADLIRGKDVSVAMMYLKFCRKKSAGYISKVLKSAVANAQANYNIDLDNLYVKEVLVGKSFSLRRIHARARGKACRVYKHYGNVIIKLFERI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that can perform rRNA binding, structural constituent of ribosome in large ribosomal subunit to support translation.2. For general function, the protein need meet that The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome. The designed protein sequence is ",
    "response#1": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#3": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG"
  },
  {
    "reference": "MNDKAKKPARTTKPRPTKESARESAKESTRERAPRGESSGDHLPPIDFDAPSEAVKAGAQPAEPLLVVPAEKRVRIGITGEPGAGKSALARQLGALGGTVIDVDRLGHELLEHPRIKRDLARAFGEDILTGDGEIDRRAVAAKAFASAEATATLNGIMHPPIAARAKALAAKAGNFVVVDAGLLHEIGLGEDCTISIYVKANRDNRVNRVAERGWDENELARREQALGDADARRKKCNLAVDNTGDPNLLAAYAKTILARQIGLDLSALKKQAPMPMRNVDNGNGNGSGDHDDDSQDNNDDASTDGDAGDQNDRAPQPEPQQQRRDQQRPGDRRFDQRGNPQQQNRQGGQQRDGGQQRDGGQQRDGGQQRDGGNQQGGNQQGGQPGNTQQREGQPGNQGNDNRPPVKIILDDYLRRTLPDLQGEADKLDVRDVKWLKKHDLICEILRRVAAGRADEIVVEGYIELIKDQQAYIRSPLNNYLPVNTDAFIANPQIRRYGLKAGMQVAGLARAPRGNERCPQLLSINTVMDEPENKRTAVQDFEHLVPLHAHERLFMELPGDPTDYSLRIIDLVAPIGKGQRGLIVAQPKCGKTVYMTKIAKAITTNNPEVVLIVLLVDERPEEVTDMQRSVRGEVVASTFDQPASRHVQCAEMVINKAKRLVERGKDVVILLDSITRLARAYNSEAPNSGRILSGGIDSNALIKPKQFFGAARKIENGGSLTILATAIVDTGSLMDTVIFEEFKGTGNMELVLDRRLANRRVFPAVDCA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. Ideal protein should have a binding affinity for key enzymes in the Cofactor biosynthesis, CoA from (R)- pantothenate: step 5/5 pathway to enhance the overall efficiency of the pathway.2. The protein design must be able to perform dephospho-CoA kinase activity, hydrolase activity in order to contribute to DNA-templated transcription termination.3. For general function, the protein need meet that Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA- dependent ATPase activity, and release of the mRNA from the DNA template.4. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "MRVLDLMTPFGKGQRGLIVAPPRGGKTVLLKTIARSIRANYPEVELIVLLLDERPEEVTDFEETVDAPVFASTFDEPSRRHAQVSDLVIERAKRLVEMGRDVVILLDSLTRLARGYNANQTGGRIMSGGLGSNALEKPRKFFSAARNVEEGGSLTIIATCLVDTESRMDEVIFEEFKGTGNLEIRLDRELSERRIYPAISLSQV",
    "response#2": "MRVLDLMTPFGKGQRGLIVAPPRGGKTVLLKTIARSIRANYPEVELIVLLLDERPEEVTDFEETVDAPVFASTFDEPSRRHAQVSDLVIERAKRLVEMGRDVVILLDSLTRLARGYNANQTGGRIMSGGLGSNALEKPRKFFSAARNVEEGGSLTIIATCLVDTESRMDEVIFEEFKGTGNLEIRLDRELSERRIYPAISLSQKGQRGIIVAPPRGGKTILLKQIARSIRLNHPDSELIILLLDERPEEVTDFEETVGAQVYASTFDESPRRHAQVADLVLERAKRIVEQGKDVILLLDSLTRLARGHNSAIQGGPIGSGGVNPVALQKSRKFFGTARNVEEGGSLTILATALIETESRLDDVVFEEFKGTGNMEVRLDRELAERRVGLVCEGVLEQAKDNFSMLRDPARSFRPSPDDIHVGANLVRDFGLRIGQRVKVRVRAPRERDKYLSGFEVIEVEGKGEGILEQAKENYAMLRDPKRSFRTSPDDFYIGGNLLKQHGLRAGQHIRVRLRAPRDRDKYLSALEVLTVEGAPGD",
    "response#3": "MRVLDLMTPFGKGQRGLIVAPPRGGKTVLLKTIARSIRANYPEVELIVLLLDERPEEVTDFEETVDAPVFASTFDEPSRRHAQVSDLVIERAKRLVEMGRDVVILLDSLTRLARGYNANQTGGRIMSGGLGSNALEKPRKFFSAARNVEEGGSLTIIATCLVDTESRMDEVIFEEFKGTGNLEIRLDRELSERRIYPAISLSQGLVCEGVLEQAKDNFSMLRDPARSFRPSPDDIHVGANLVRDFGLRIGQRVKVRVRAPRERDKYLSGFEVIEVEGKG"
  },
  {
    "reference": "MAKTRKKHIKYIFVVGGVMSGVGKGITTSSIGKILQAYGLGVTAFKIDPYINVDAGTMNPTEHGEVFVLKDGYETDQDMGNYERFLNIELSRDNYMTTGMVYKSVIERERNLEYVGKCVQVVPHIPLEVIARIKRTAKKDKADVAIIEIGGTVGEYENILFLEAIRMMKIESPKDVITVMVSYIPTPSTIGEMKTKPTQHAVRTLNGTGIQPDIIVARASRPLDEKRKGKIAIFCSVRKEDVISAPDVESIYDVPLNFEKDHLSAILFHKLGLKPRKQNDTLEEWARVVKRIHSATDEVHIGIVGKYFKTGDYVLSDAYISVIEALKHAACKIGKKAILTWIDSTDFEEDSKKLATLKQYNGIIIPGGFGKRGIEGKLAVIRYCRLKKLPYFGLCYGMQLLVVEYARDVLGLRDAHSTEVSPDTKHPVVDIMPEQKENMRKKNYGATMRLGHYLAHIFPKTIAWSAYGTENILERHRHRYEVNPEYVEDLKKGGLVFSATSPDGVLMEIAELPKKNHPFFLATQFHPEFQSSLLHPHPLFVAFLKACMKKQ",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The ATP, CTP, UTP, L-glutamine, Mg(2+) binding site should be stable and able to withstand changes in environmental conditions.2. A protein that can modulate the overall flux of metabolites through the CTP from UDP: step 2/2, Pyrimidine metabolism, CTP biosynthesis via de novo pathway pathway, enhancing or suppressing its activity as needed.3. The designed protein should contain one or more Glutamine amidotransferase, CTP synthase N-terminal domains that are essential for its function.4. The protein design must be able to perform ATP binding in order to contribute to glutamine metabolic process.5. The protein must exhibit the following characteristics: Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates..6. The protein with the reaction H2O + L-glutamine = L-glutamate + NH4(+), the active site, Nucleophile; for glutamine hydrolysis, Nucleophile must be optimized to ensure efficient catalysis. The designed protein sequence is ",
    "response#1": "MPDAYLSLLEALKHGGLKNQARVEIKWVDAEGLANGDLEEAFRDVDGILVPGGFGVRGIEGKVRAAQYARENGVPYLGICLGLQVAVIEFARNVAGLEKANSTEFDPYTPHPVIDLMPEQLEVEDLGGTMRLGDWPMQIKPDTLLARVYEGKPLVRERHRHRYEVNPAFVDRLTQAGLVVSGVTPGMEGRGEGLVEAIELPDHPFFLALQSHPEFKSRPMRPSPPFAAFIAAARAALSPA",
    "response#2": "MPDAYLSLLEALKHGGLKNQARVEIKWVDAEGLANGDLEEAFRDVDGILVPGGFGVRGIEGKVRAAQYARENGVPYLGICLGLQVAVIEFARNVAGLEKANSTEFDPYTPHPVIDLMPEQLEVEDLGGTMRLGDWPMQIKPDTLLARVYEGKPLVRERHRHRYEVNPAFVDRLTQAGLVVSGVTPGMEGRGEGLVEAIELPDHPFFLALQSHPEFKSRPMRPSPPFAAFTAAMKAAAA",
    "response#3": "MPDAYLSLLEALKHGGLKNQARVEIKWVDAEGLANGDLEEAFRDVDGILVPGGFGVRGIEGKVRAAQYARENGVPYLGICLGLQVAVIEFARNVAGLEKANSTEFDPYTPHPVIDLMPEQLEVEDLGGTMRLGDWPMQIKPDTLLARVYEGKPLVRERHRHRYEVNPAFVDRLTQAGLVVSGVTPGMEGRGEGLVEAIELPDHPFFLALQSHPEFKSRPMRPSPPFAAFVAAAEAALTR"
  },
  {
    "reference": "MDFESFTLLAATDDLGVEPAARVDADGLELRMDFADEPLAQLDAYDGDLPILVTNRPTWEGGEAADTAGRLDALETALEHDAVTAVDLELAALEGAGDHDAGRVADAARDRGASVVVSTHNFESTPDREAIVSRLERACAHGDVGKMASTAQSPDDVLAMLGATRELTAEGEQVATMCMGAAGRHSRAVAPVYGSRIGYAPVDPADATAPGQYDLATLRTLVGQLQSDA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. We require a protein that can specifically bind to the metabolites produced in the chorismate biosynthesis, Metabolic intermediate biosynthesis, chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7 pathway.2. The protein design should aim to mimic or enhance the function of natural proteins involved in aromatic amino acid family biosynthetic process, chorismate biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.3. The Proton donor/acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.4. For general function, the protein need meet that Catalyzes the cis- dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate. The designed protein sequence is ",
    "response#1": "GMAEVVKTAAIWNEAEFTYLEENAALVIQTVRGRPVQATSEHLVPIRGILKRIVLASARVKAEVVSADEKEGGLRNLLNFGHSIGHAYEAILTPQVLHGEAVAIGMVKEAELARYLGVLRPSAVARLVKCIASYDLPTSLADKRIVALTAGKKCPVDVVLDKMAVDKKGFVVIN",
    "response#2": "MPPSDLNVTVAPPGSKSISNRALVLAALANGTCRLRNLLRSDDTEYMLSAILQLKGATYSWEESGEVLSVQGNGGNFLASSEPLYLGNAGTASRFLTSVVALCSPSAVSSTVLTGNDRMKVRPIGPLVDALRSNGVKVKYLGKDKSLPLEVDAAGGLEGGLIELAATVSSQYVSSILMAAPYAKNPVVLRLVGGKPISQPYIDMTISMMRAFGVLVKASDSEPNTYHIQTGVYTSPAEYVVESDASSATYPLAVAAITGSTCTIPNIGSASLQGDASFAVSVLQPMGCAVTQTETSTTVTGPPIGSLKAIAHVDMEPMTDAFLTASVLAAVASGTTRITGIANQRVKECNRILAMYDEIV",
    "response#3": "GMAEVVKTAAIWNEAEFTYLEENAALVIQTVRGRPVQATSEHLVPIRGILKRIVLASARVKAEVVSADEKEGGLRNLLNFGHSIGHAYEAILTPQVLHGEAVAIGMVKEAELARYLGVLRPSAVARLVKCIASYDLPTSLADKRIVALTAGKKCPVDVVLDKMAVDKKY"
  },
  {
    "reference": "MGFTKIVVTFFLVVMLAVSSSSQNAMASEIKAKINGLECFNTCTPYYDDYKCNVDCLSSGYPAGDCHIVSPSQPKKCCCY",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein design should aim to mimic or enhance the function of natural proteins involved in defense response to fungus, killing of cells of another organism, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "MS",
    "response#2": "GTCQSQSHKFKGACFSDTNCDSVCRTENFPRGQCNQHHVERKCYCERDC",
    "response#3": "VCMGKSQHHSFPCISDRLCSNECVKEDGGWTAGYCHLRYCRCQKAC"
  },
  {
    "reference": "MEKLTILLLVAAVLMSTQALVERAGENRSKENIKFLLKRKRAADRGMWGKCKDGLTTCLAPSECCSGNCEQNCKMW",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must have high specificity towards ion channel inhibitor activity, toxin activity.2. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "RSPWFDPVRRCCSQDCRVCIPCCPR",
    "response#2": "LSSG",
    "response#3": "MPNCRYFLGECKKTSECCEHLACHDKHKWC"
  },
  {
    "reference": "MLCLHNKICRNKLHQNILAETEPRTTISFYKYFDIQNPNLFRDRFYLQMNELKIFGRIYIAKEGINAQISVLNSRINAFRTMLYTSHPQLDQLRLNFALDNNRQSFWMLRMKVKKLLVADGIQEPNFNCFNANNYLKAEEVNILLENPNILFIDMRNNYEYEVGHFQHAMEIPSRTFREKLPIAIKMLQHNKERKIVMYCTGGIRCEKASAWMLHNGFQHIFQVEGGIIGYVNRAVKLGLPLKFIGKNFVFDDRLGERITSDVIAYCHQCKNSCDDHTNCLNQDCHNLFIQCRQCTKKYDGCCSLSCQQTLRTRITRLKNNFIK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The Cysteine persulfide intermediate of the protein must be engineered to enable effective catalysis of AH2 + O2 + uridine(34) in tRNA = 5-hydroxyuridine(34) in tRNA   + A + H2O.2. The designed protein should have oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen to facilitate tRNA modification. The designed protein sequence is ",
    "response#1": "PEDWNDLIRSDDVVLIDTRNDYEVAIGTFEGAIDPMTESFRDFPAWWEANKERFHNKRVAMFCTGGIRCEKSTNYLLGQGVEDVYHLKGGILRYA",
    "response#2": "PEDWNQLLADPDVVVIDVRNDYEVAIGTFKGAINPNTKSFSELPEWLQEQAELQKKPKVAMFCTGGIRCEKSTALLRHEGFEDVFHLQGGILSYAK",
    "response#3": "PEDWNQLLADPDVVVIDVRNDYEVAIGTFKGAINPNTKSFSELPEWLQEQAELQKKPKVAMFCTGGIRCEKSTALLRHEGFEDVFHLQGGILSYV"
  },
  {
    "reference": "MTEQTPAPQPPADENSLIAERRAKLGALRGQGIAYPNDFVREHFAGDLQAEFADADTWTPEALEASGRTVRMAGRLMAKRVMGKASFAQIQDESGRVQLFLQGNVLGDAYTAFKGWDVGDIVAVEGGLTRTKTGELSVKASALRLLTKSLRPLPDKWHGLSDVEQRYRQRYVDLIVTPEAREVFIKRSKIIRAMRAWLDARRFLEVETPMMHYIPGGATAKPFTTHHNALDLDLYLRVAPELYLKRLVVGGLERVYEINRNFRNEGVSTRHNPEFTMLELYEAYATYHQIMDLTEQVIRDTAQSVLGTTQVSWDGADIDLAPAFRRWRMDEAVRHHNPEISAADCTDREALLRHCERLKIRVKPSYGWGKLLLEIFEATVEHTLVQPTFITDHPVEVSPLARSSDTEPGYTDRFELFINGKELANGFSELNDPEDQAARFQAQVQAKDGGDDEAMHFDADYIRALEYGMAPTGGLGIGIDRLVMLLTGSTSIRDVLLFPYMRPEA",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. A protein with magnesium ion binding is needed to enhance lysyl-tRNA aminoacylation in a specific context. The designed protein sequence is ",
    "response#1": "FFGELGFSVESGPEIEDDYHNFDALNIPAHHPARADHDTFWFDAKRLLRTQTSGVQIRTMQNKQPPIRIIAPGRVYRNDYDQTHTPMFHQVEGLIVDKDISFTNLKGTLHDFLKNFFEEDMEIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPEVYSGFAFGMGMERLTMG",
    "response#2": "FFGELGFSVESGPEIEDDYHNFDALNIPAHHPARADHDTFWFDAKRLLRTQTSGVQIRTMQNKQPPIRIIAPGRVYRNDYDQTHTPMFHQVEGLIVDKDISFTNLKGTLHDFLKNFFEEDMEIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPEVYSGFAFGMGMERLTME",
    "response#3": "DFTVAEETQQMLQEMSASQELSHLVPERVWREWEKSLLTQTPTVFLTLLRDIQALSQVLPGITS"
  },
  {
    "reference": "MNTKFPITAKGFEKLEHELKHLKHVERKKISADIAEAREHGDLSENAEYEAAREKQAFIEGRIKELEDMTARAEIIDIGKLSGDNIKFGATVTLIDDDTEEEVTYIIVGEYEADITRKRVSIASPIAKALIGKSVGDFVEVTTPKGSKSYEVVTVEYKELEL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit DNA binding.2. The protein must exhibit the following characteristics: The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes; GreA releases sequences of 2 to 3 nucleotides.. The designed protein sequence is ",
    "response#1": "MRS",
    "response#2": "SSISSPIARALIGKGEGA",
    "response#3": "MSIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSENAEYEAAKERQGFIEGRISELEHKLSVAHI"
  },
  {
    "reference": "MPTINQLVRHGRKVIKEKSKSPALQGHPQKRGVCVRVSTMTPKKPNSALRKIARVRLSNGIEVTAYIPGIGHNLQEHSVVLVRGGRVKDLPGIRYKIIRGALDTDGVENRKQSRSKYGTKRPKK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together; The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit.; Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone2. The designed protein should have structural constituent of ribosome, tRNA binding, rRNA binding within small ribosomal subunit to regulate translation. The designed protein sequence is ",
    "response#1": "MTPPIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTAV",
    "response#2": "MPIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTG",
    "response#3": "MTPPIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTCGQ"
  },
  {
    "reference": "MLKGRIALNILQSQKPIVFSASQQRWQTNVPTAEIRNDPEWLQAKPFEEIPKANILSLFAKSALPGGKYKNLEMMEMIDALRQDYGNIIFLPGMMGRDGLVMTHNPKDFEVVFRNEGVWPFRPGSDILRYHRTVYRKDFFDGVQGIIPSQGKSWGDFRSIVNPVLMQPKNVRLYFKKMSQVNQEFVELIKEIRDASTQEVPGNFLETINRWTLESVSVVALDKQLGLLRESGKNSEATKLFKYLDEFFLHSADLEMKPSLWRYFKTPLLKKMLRTMDSVQEVTLKYVDEAIERLEKEAKEGVVRPEHEQSVLEKLLKVDKKVATVMAMDMLMAGVDTTSSTFTALLLCLAKNPEKQARLREEVMKVLPNKDSEFTEASMKNVPYLRACIKESQRVYPLVIGNARGLTRDSVISGYRVPAGTIVSMIPINSLYSEEYFPKPTEFLPERWLRNASDSAGKCPANDLKTKNPFVFLPFGFGPRMCVGKRIVEMELELGTARLIRNFNVEFNHSTKNAFRSALINLPNIPLKFKFTDVPN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein that localizes to mitochondrial membrane and can perform heme binding, monooxygenase activity, oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, iron ion binding with high specificity and efficiency.2. The presence of heme is crucial for the protein to function optimally under specific conditions.3. The protein should have a high affinity for heme. The designed protein sequence is ",
    "response#1": "RIVPTTVALTMTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDEMQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKAAKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLNINPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDADKAMTQLYDLFYKDK",
    "response#2": "TLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDEMQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKAAKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLNINPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLED",
    "response#3": "RIVPTTVALTMTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDEMQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKAAKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLNINPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDADKAMTQLYDLFYKDA"
  },
  {
    "reference": "MKEVNIDTLISKIPNKYVLTVVISKRARQLFEELKFLKTIARDPLILAMEEIAQEKIAYGEGDDLED",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should be expressed specifically in the DNA-directed RNA polymerase complex The designed protein sequence is ",
    "response#1": "RSPGAYYKIDLNRKNGENVYYVDLIPSRGTWLEFESDHKKIKVGKEEKFENVFYVKIDKSRKVSVANFLTALGIIKEDALDIFGDNKLVKSTYELDPYTGDILYDQSIAVQEIYKKIRSGETATPDGATKYLYGLLFDKRKYDLTKAGRFKLIQKLSVENRIYNKILAEDR",
    "response#2": "AEDPGIVGDVSADVVTVKQDDGKHRDYHVGKFVRSNQGNCYNQRVVVRSGDRVEKGTVLADGPCTDKGELSLGRNLLVAFMPWEGYNFEDAIIISQNLVKDDTLSSIHIEEHEVSTRDTKLGSEEITRDLPNVV",
    "response#3": "RSPGAYYKIDLNRKNGENVYYVDLIPSRGTWLEFESDHKKIKVGKEEKFENVFYVKIDKSRKVSVANFLTALGIIKEDALDIFGDNKLVKSTYELDPYTGDILYDQSIAVQEIYKKIRSGETATPDGATKYLYGLLFDKRKYDLTKAGRFKLIQKLSVENRIYNKILAEDC"
  },
  {
    "reference": "MFELYLIIITCLLLLVCYLITNSLVSIYTQIRVINNIEPIKDKISLGNDYILYMTRLYMKRKKWLYCITMLEFYINQIKIHETEITGEYYNCIGSCYQAIKMYKIAKKYYLKAYKRTPSRQHILKNLANIYNTVGDIKNAKKIYQRLT",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be expressed specifically in the chloroplast The designed protein sequence is ",
    "response#1": "IKPYNDDPFVGHLATPITSSAVTRAILQNLPAYRFGLTPLLRGLEIGLAHGYFLMGPFVKLGPLRDSEIGLLAGFLSTVGLIVILTLGLTIYGVAAFGQEKTQSSNENDLQTKKAWDQFKGGFFVGACGSAGFAFICLSSS",
    "response#2": "IKPYNDDPFVGHLATPITSSAVTRAILQNLPAYRFGLTPLLRGLEIGLAHGYFLMGPFVKLGPLRDSEIGLLAGFLSTVGLIVILTLGLTIYGVAAFGQEKTQSSNENDLQTKKAWDQFKGGFFVGACGSAGFAFICLSSL",
    "response#3": "VLFLPGFGVGSFHYEKQLTDLGRDYRVWAIDFLGQGLSLPTEDPTTMTEETSSSEDKEPFWGFGDKTEPWADQLVFSLDLWRDQVQYFVEEVIGEPVYIAGNSLGGYVALYFAATHPHLVKGVTLLNATPFWGFFPNPVRSPKLARLFPWPGAFPLPERVKKITELVWQKISDPESIAEILKQVYTDHSINVDKVFSRIVEVTQHPAAAASFASIMLAPGGELSFSEALSRCKENNVQICLMYGREDPWVRPLWGKKIKKEIPNAPYYEISPAGHCPHDEVPEVLNSL"
  },
  {
    "reference": "MQKFEGYSEKQKSRQHYFVYPLLFQEYIYAFAHDYGLNGSEPVEIFGCNNKKFSSILVKRLIIRMYQQNFLINSVNYPNQDRLFDHRNYFYSEFYSQILSEGFGIVVEIPLSLGQLSCPEEKEIPKFQNLQSIHSIFPFLEDKFLHLHYLSHIEIPYPIHLEILVQLLEYRIQDVPSLHLLRFFLHYYSNWNSLITSMKSIFLLKKENQRLFRFLYNSYVSEYEFFLLFLRKQSSCLRLTSSGTFLERIIFSGKMEHFGVMYPGFFRKTIWFFMDPLMHYVRYQGKAILASKGTLLLKKKWKSYLVNFSQYFFYFWTQPQRIRLNQLTNSCFDFLGYLSSVPINTLLVRNQMLENSFLIDTRMKKFNTTVPATPLVGSLSKAQFCTGSGHPISKPVWTDLSDWDILDRFGRICRNLFHYYSGSSKKQTLYRLKYILRLSCARTLARKHKSTVRTFMQRLGSVFLEEFFTEEEQVFSLMFTKTIHFSFHGSQSERIWYLDIIRINDLVNPLTLN",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein must exhibit the following characteristics: Probably assists in splicing its own and other chloroplast group II introns..2. The protein should be able to modulate mRNA processing in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "TMISIIPMIGSLAKAKFCNLSGNPISKPAWADLSDSDIIDRFGRIYRNLSHYYSGSSKKQSLYRIKYILRLSCARTLARKHKSTVRAFLQRLGSEFFEEFFMEEDKVR",
    "response#2": "TMISIIPMIGSLAKAKFCNLSGNPISKPAWADLSDSDIIDRFGRIYRNLSHYYSGSSKKQSLYRIKYILRLSCARTLARKHKSTVRAFLQRLGSEFFEEFFMEEDKVH",
    "response#3": "TMISIIPMIGSLAKAKFCNLSGNPISKPAWADLSDSDIIDRFGRIYRNLSHYYSGSSKKQSLYRIKYILRLSCARTLARKHKSTVRAFLQRLGSEFFEEFFMEEDKVG"
  },
  {
    "reference": "MGYRVAVVGATGAVGREMLQTLAERNFPADKVHALASSKSAGRELSYGEDVTLTVEALDKFDFASADIALFSAGGDTSKAFAPKAAEAGCLVIDNSSAYRMDADVPLIVPEVNADAVSGVTRANGGRNIIANPNCSTAQLVVALKPLHEAFGVRRVVVSTYQAVSGAGRPAMDELFNQTRGVFVNDSVQPELFTKQIAFNAIPHIDVFLDDGFTKEEWKMSAETKKILDPSIELVAQCVRIPVFIGHSEAVFIETDRPMAENAARKLLQDAEGCIVVDHRANEGYVTPQEAAGEDAVYISRIRKDPTIDNGLVFWCVSDNLRKGAALNSVQIAELVVARGISGR",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must have a Proton acceptor, Acyl-thioester intermediate active site that is highly conserved among related enzymes.2. A protein should be able to manipulate the activity of enzymes in the Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5, L-threonine biosynthesis pathway, either by activating or inhibiting their function.3. The designed protein must possess NAD binding, aspartate-semialdehyde dehydrogenase activity, protein dimerization activity, NADP binding to support threonine biosynthetic process, lysine biosynthetic process via diaminopimelate, diaminopimelate biosynthetic process under certain conditions.4. The designed protein should contain one or more Semialdehyde dehydrogenase NAD-binding domains that are essential for its function.5. The designed protein should have a high specificity for phosphate binding, with minimal off-target effects. The designed protein sequence is ",
    "response#1": "VAVLGATGTVGQHFVKLLLNHPWFELAALTASERSAGKRYGDAAKWYLSEEVPEQVADVEVRLTEPRAVEDAKLLFSAMPADEAAKVEPACAEAGFIMSSNSSNFRMDPDVPLIIPEVNPDHLLKPIDDAVGIERVVVATYQAVSGAGSRAIHELGETTRAVFTQQEVAPAVFPKRIAFNCLPQIDVFQDNGYTKEEMKMLWESRKILERPELALTATCVRVPVFYGHSEAVNVVTREKMRAEELRSLLQRAPGVRVLDERTAGGWPTALDAAGQDATWVGRIREDCSHERGLNFWVVADNIRDAHHKGKIVAGTCVRVPVFTGHLKPIDDAVGIERVVVATYQAVSGAGSRAIHELGETTRAVFTQQEVAPAVFPKRIAFNCLPQIDVFQDNGYTKEEMKMLWESRKILERPELALTATCVRVPVFYGHSEAVNVVTREKMRAEELRSLLQRAPGVRVLDERTAGGWPTALDAAGQDATWVGRIREDCSHERGLNFWVVADNIRGAADVKPVLDVQTARGQRHDEVEAAYEVAKELGLY",
    "response#2": "LGIVGATGQVGVAVREILLERDFPIGQVRFFASARSAGTVLSFGDREITVEDASSADPAGLDIAIFSAGATTSRALAEKFAEAGVIVIDNSSAFRKDPAIPLVVSEVNPSAMAGFTKKGIIANPNCSTIQMLVALKPLLDAFGINSVFVASMQALSGAGYAGVPSMAIEDNVIPFIAKEEDKAEDEPLKLLGKLSGKDIEQAPIRISASCHRVPVLDGHTMAVFVELGKRASPEDVAEVLSKFKAKPQKLKLPSAPEQPVIVSPEEDMPQPRYARYAGSVPGMAVVVGRVRKDPALKNGIKFIASGHNVRRGAALNSVQIAELLV",
    "response#3": "MAANVAVVGATGAVGEAMLSILEERNFPVGEVYALASSKSVGKRIPFKGGSLKVEDLADFDFSKAQIGLFSAGGSVSAEYAPKAAAAGCIVVDNTSHFRYDDDIPLVVPEVNPEKIADCTTNCVVPMMLKPIYDAVGITRINVCTYQAVSGTGKKGIEEVVKQTSRLLNGQPIETNVYPKQIAFNVLPQIDVFMDNGYTKEEMKMVWETQKILDDQSIMVNATAVRVPVFYGHSEAVHIETQKKIDADTVRSLLSKAPGVAVLDERKNGSYPTAVTESSGHDNVFVGRIREDISHPLGISLWVVGDNIRYDNEYGYSTRVVDLVDYAAQQ"
  },
  {
    "reference": "MADRREKSADQMKLWKESRANQKPDVLTTGGGNPVSDKLNLLTVGPRGPLLVQDVVFTDEMAHFDRERIPERVVHAKGAGAFGYFEVTHDITRYSKAKVFEFIGKRTPIAVRFSTVAGEAGSADTVRDPRGFAVKFYTEDGNWDLTGNNTPIFFVRDAMLFPSFIHSQKRNPQTHMKDPDMVWDFWALRPESLHQVSFLFSDRGIPDGHRHMNGYGSHTFKLVNAKDEPIYCKFHFKTDQGIRNLTVEEANRLSAEDPDYGIHDLYEAIANGNYPSWTFYIQVMTFEQAERYPFNPFDLTKIWPHKDYPLIPVGKLVLNRNPANYFAEVEQIAFDPSNMPPGIEPSPDKMLQGRLFSYPDTHRHRLGANYLQLPVNCPYKARVANYQRDGPMCFSDNQGGAPNYFPNSFSAPENQPAARESKFRVSADVARYNSSDDDNVSQVRDFYTKVLSEEERKRLCENIAGHLKGAQIFIQKRAVKNFTDVHPDYGNRVQALLDKYNAEGHHKKVIKTYTQHSAHVTANDKANL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.2. A protein that can perform catalase activity, heme binding, metal ion binding in peroxisome to support response to oxidative stress, hydrogen peroxide catabolic process.3. The designed protein should have a binding site that is specific to the target NADP(+), heme ligand.4. For general function, the protein need meet that Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide.5. The protein must bind to NADP(+) in order to perform its enzymatic function.6. The designed protein should contain at least one Microbody targeting signal; atypical motif to achieve its intended function.7. A key design requirement for the protein is the optimization of the active site to achieve high catalytic efficiency for 2 H2O2 = 2 H2O + O2. The designed protein sequence is ",
    "response#1": "LDKARRLLWPIKQKYGNKVSWADLMILAGNMAYESMGLKTFGFAFGREDIWHPEKDTYWGAEKEWLAPSGGANSRYSGVRDLQNPLAAVMMGLIYVNPEGVDGNPDPLKTAQDMRVTFARMAMNDEETVALTAGGHTVGKAHGNGNAANLGPEPEAAPLEEQGFGWMNHQSRGIGRNTVTSGIEGAWTTHPTRWDNGYFHLLFSYDWALTKSPAGAWQWEPVNIREEDKPVDVEDPNIRSNPMMTDADMALKMDPDYRRIAERFFHDPDYFADTFARAWFKLTHRDMGPKSRYFDELASTLAKIDSLRGSLSMADAIVLAGSAAVEKAAKDAGFDISVPFTGGRGDASEEQTDAESFEPMEPHADGFRNYLKTKHSVKTEEMLLDRAQLLGLTIPEMTVLVGGLRVLGANHSSAGDEGQFTDRPGRLTNDFFVNLLDMNTAWEVVDESGDEKFVGTDRATEEHRWNATRTDLIFGSNSQLRAVAEVYAENGNEEKFVRDFVAAWTKL",
    "response#2": "ADYGHYGGLFIRMAWHSAGTYRTADGRGGGGTGNQRFAPINSWPDNVNLDKARRLLWPIKQKYGRKISWADLMILAGNVAMESMGFKTFGFGGGRADIWEPEKDIYWGAEEEWLANQRYSGERDLENPLAAVQMGLIYVNPEGPDGNPDPIASGKDVRETFKRMAMNDEETVALTAGGHTFGKCHGAGDPANVGPEPEGAPIEQQGLGWKSSYGSGVGADQIGSGLEGAWNPTPTRWDMGYFDMLFGNEWELVKSPAGAQQWTPKKSTDDNMAPAPDDGSKKVPIMMTTADMSMRMDPAYEKISRRFHQADYGHYGGLFIRMAWHSAGTYRTADGRGGGGTGNQRFAPINSWPDNVNLDKARRLLWPIKQKYGRKISWADLMILAGNVAMESMGFKTFGFGGGRADIWEPEKDIYWGAEEEWLANQRYSGERDLENPLAAVQMGLIYVNPEGPDGNPDPIASGKDVRETFKRMAMNDEETVALTAGGHTFGKCHGAGDPANVGPEPEGAPIEQQGLGWKSSYGSGVGADQIGSGLEGAWNPTPTRWDMGYFDMLFGNEWELVKSPAGAQQWTPKKSTDDNMAPAPDDGSKKVPIMMTTADMSMRMDPAYEKISRRFHQADYGHYGPLFIRMAWHSAGTYRMGDGRGGAGSGSQRLAPLNSWPDNVNLDKARRLLWPIKQKYGKRISWADLMVLAGNCAIESMGLPTFGFAGGREDVWEPEQDVYWGSEEEWLATSDKPKSRYSGERDLENPLAAVQMGLIYVNPEGPDGNPDPIASGKDVRETFARMAMNDEETVALVAGGHTFGKCHGAGDAALVGPEPEAAPLEEMGLGWKSSHGRGKGGDTISSGIEGAWKPRPTTWDMGYLKVLFKYDWELVKSPAGANQWLAKDVDDEDMVVDAHDPDKKHRPMMTTADLSLKFDPIYEPIARRYLE",
    "response#3": "AFARAWFKLTHRDMGPRARYLGPEVPEEDLIWQDPIPAVDHELIDDKDIADLKAKILASGLSVSQLVTTAWASASTFRGSDNRGGANGARIRFAPQKDWAVNQPAELQKVLQALEGIQKDFNAGQSGGKKVSLADLIVLGGCAGVEKAAQNAGADAAVPFAPGRMDALEEQTDGDSFSVLEPKADGFRNFQKAKFAVKAEELLVDRAQLLTLTAPEMTVLVGGLRMLGANYGNSKHGVFTDKSETLTNDFFVNLLDMGTVWKPSANDEDVFEGSDRKTGALKWTGTRVDLVFGSNSQLRAIAEVYGSMEAPKQ"
  },
  {
    "reference": "MFATIEDALKDIRLGRMVIIVDDETRENEGDLVFAAQKVSAQKVNFMAKHGRGLVCVSVTGKRASELGLFAQSSTNTALHGTAFTVSVDAVRGTTTGISAHDRAATIRALADPKTKPGDLARPGHIFPIVAQAGGVLVRAGHTEASTDLVRLAGLKPVGVLCEVMAENGRMARTRALRGLAKRFGLKVITIKDLIAYRGQREKLVHKIVETDLPTEYGEFRVHVYEDMLDSYAHLALVKGHVRGKKRVLVRVHSQCLTGDVFHSLRCDCGQQLAKSMQMIDKEGLGVLLYMRQEGRGIGISNKLQAYALQDRGLDTVDANTALGFAPDLRDYGIGAQILVDLGLSSIRLLTNNLRKVVGLRGFGLKIVERVPIVVKANKLNIRYLITKRDRLGHLLGDIEKGVKDGKKGISGKA",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The design of the protein must focus on the precise arrangement of amino acids in the Proton acceptor; for GTP cyclohydrolase activity to enable effective catalysis of GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.2. The protein must exhibit zinc ion binding, manganese ion binding. The designed protein sequence is ",
    "response#1": "KMAETNLPTEYGKFKLFVYNSDVDYLPHLALVLNKITNPSLVRVHSECLTGDVFMSKRCDCGRQLHKSMRIIQKNGQGVLLYMRQEGRGIGLTNKIKAYALQDHGFDTVEANEKLGFKPDLRDYGIGAQILSDLGIQKIRLLTNNPYKIIGLEGYGIEIVERVI",
    "response#2": "KMAETNLPTEYGKFKLFVYNSDVDYLPHLALVLNKITNPSLVRVHSECLTGDVFMSKRCDCGRQLHKSMRIIQKNGQGVLLYMRQEGRGIGLTNKIKAYALQDHGFDTVEANEKLGFKPDLRDYGIGAQILSDLGIQKIRLLTNNPYKIIGLEGYGIEIVERVD",
    "response#3": "KMAETNLPTEYGKFKLFVYNSDVDYLPHLALVLNKITNPSLVRVHSECLTGDVFMSKRCDCGRQLHKSMRIIQKNGQGVLLYMRQEGRGIGLTNKIKAYALQDHGFDTVEANEKLGFKPDLRDYGIGAQILSDLGIQKIRLLTNNPYKIIGLEGYGIEIVERVI"
  },
  {
    "reference": "MFSKSGMSDLMKHAQRMQEQMQQMQGEIAKLEVNGESGAGLVKVTINGVYHCRRVEIDSSLLQDDKDMLEDLITAAFNDAVRRITEIKKDKMTSLSSSLQMPLGFNWNMPFL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein can be targeted to bacterial nucleoid and perform DNA binding.2. The protein must exhibit the following characteristics: May be involved in regulation of gene expression, nucleoid organization and DNA protection.. The designed protein sequence is ",
    "response#1": "EMNXTXWX",
    "response#2": "GXAVXPX",
    "response#3": "MI"
  },
  {
    "reference": "MLQPARRKYRKEQKGRNTGISHSRGTAVSFGEFGLKAIGRGRITARQIEAARRAMTRHIKRGGRIWIRIFPDKPISQKPAEVRMGNGKGNPEYYVAEIQPGKMLYEMDGVDEALAREAFRLAAAKLPLLTTFVVRQVGQ",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that localizes to ribonucleoprotein complex, ribosome and can perform rRNA binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "MGNALPLTDMSLGTAIHNIEITLGKGGQLARAAGTVAKLIAKEGKSATLRLPSGEVRLVSKNCSATVGQVGNVGVNQKSLGRAGSKCWLGKRPVVRGVVMNPVDHPHGGGEGRAPIGRKRPTTPWS",
    "response#2": "MGNALPLTDMSLGTAIHNIEITLGKGGQLARAAGTVAKLIAKEGKSATLRLPSGEVRLVSKNCSATVGQVGNVGVNQKSLGRAGSKCWLGKRPVVRGVVMNPVDHPHGGGEGRAPIGRKRPTTPWG",
    "response#3": "MGNALPLTDMSLGTAIHNIEITLGKGGQLARAAGTVAKLIAKEGKSATLRLPSGEVRLVSKNCSATVGQVGNVGVNQKSLGRAGSKCWLGKRPVVRGVVMNPVDHPHGGGEGRAPIGRKRPTTPWG"
  },
  {
    "reference": "MKFGIVARRDREEALKLAYRVYDFLKVNNYEVYVDEEAYIHFPHFSSEDVLSLKKMDVDMIIAIGGDGTVLRVEHNTSKDIPILAVNMGTLGFLAEIEPAETFFAISRILEGDYFIDERMKIRVFVEDVSIPDALNDVVILTSIPGKVTHLKYYVDGELAEDIRADGLIISTPTGSTAYALSAGGPLVDPRLHAILLVPLAPVALTARPLVVPDCSSIEIEVLTEREIVLTVDGQFYTQLPSNLKIRVEKSPRKTKFVRFSERIYPKYTLKIKKKF",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The NAD(+) binding site should be located in a region of the protein that is accessible to the ligand.2. The protein must exhibit the following characteristics: Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP..3. The protein requires a divalent metal cation to activate a critical catalytic site.4. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for ATP + NAD(+) = ADP + H(+) + NADP(+).5. The designed protein should be involved in NADP biosynthetic process by localizing to cytoplasm and exhibiting NAD+ kinase activity, metal ion binding, ATP binding. The designed protein sequence is ",
    "response#1": "MIDIVWSKDVVEAAEKYRQPFADKMVSLAEKLDVEFVGREDGLIHIDDEKALDEYRAALKDKWAYEVMRETEQEQDGTVKNMFLLSSDRHIESIVMKLHPEYGHAAFPTYNSINDPDVIAKFTAKIPMDANGKPVLDTTGKPLTPDQLKKLYTEGYAVCEFN",
    "response#2": "MKTFNLDVPRDIKV",
    "response#3": "MXXXTNXQXTXPXXFXXGXFX"
  },
  {
    "reference": "MDPVDPNLEPWKHPGSQPKTACNTCYCKKCCFHCQKCFTQKGLGISYGRKKRRQRRRSPQDNQTHQVSLSKQPTAQSGGDPTGPEKSTKKVERETETHPVP",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design should emphasize Basic and acidic residues, Polar residues compositional bias.2. For general function, the protein need meet that This binding increases Tat's affinity for a hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA) and allows Tat/P-TEFb complex to bind cooperatively to TAR RNA; Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to promote processive transcription elongation by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts; Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication; Other factors such as HTATSF1/Tat-SF1, SUPT5H/SPT5, and HTATIP2 are also important for Tat's function; In the cytoplasm, Tat is thought to act as a translational activator of HIV-1 mRNAs.3. A protein that contributes to suppression by virus of host translation initiation, positive regulation of viral transcription, negative regulation of peptidyl-threonine phosphorylation by functioning in host cell nucleolus with actinin binding, cyclin binding. The designed protein sequence is ",
    "response#1": "MEPVDPNLEPWNHPGSQPTTACNKCYCKCCSWHCQLCFLKKGLGISYGRKKRKHRRGTPQSSKDHQNPIPKQPLPTTRGNPTGPKESKKEVASKAETDPFA",
    "response#2": "MEPVDPNLEPWNHPGSQPTTACSKCYCKKCCWHCQLCFLKKGLGISYGRKKRKRRRGTPQSSKDHQNPIPKQPLSTSRGNPTGPKESKKEVASKAETDPCD",
    "response#3": "MDPVDPNLEPWNHPGSQPKTACNNCYCKKCCWHCQICFLKKGLGISYGRKKRKHRRGTPQSRKDHQNPAREQSLPIIRGKQTDSKEQKKEVASKTETDRFA"
  },
  {
    "reference": "MAATCEISNVFSNYFNAMYSSEDPTLAPAPLTTFGTEDFVLTLNNQHMSPEGPVGCVGPQTRSQRDRTEPPAVLHLAEKASWTGERPQFWSKTQVLDWISYQVEKNKYDASSIDFSRCDMDGATLCNCALEELRLVFGPLGDQLHAQLRDLTSSSSDELSWIIELLEKDGMTFQEGLGDSGPFDQGSPFAQELLDDGRQASPYYGSSYGPGAPSPGSSDFSTSGTDTPQSSHSSDSGGSDVDLDLTDSKVFPRDGFPDYKKGEPKHGKRKRGRPRKLSKEYWDCLEGKKSKHAPRGTHLWEFIRDILIHPELNEGLMKWENRHEGVFKFLRSEAVAQLWGQKKKNSNMTYEKLSRAMRYYYKREILERVDGRRLVYKFGKNSSGWKEEEVGESQN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein must exhibit the following characteristics: Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]; Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter; May be a critical downstream effector of the ERBB2 signaling pathway; Involved in mediating vascular inflammation; May be associated with mammary gland development and involution; May play an important role in epithelial cell differentiation and tumorigenesis; Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters.2. The designed protein's functionality will benefit from Basic and acidic residues, Polar residues compositional bias in its structure.3. The designed protein should have sequence-specific double-stranded DNA binding, DNA-binding transcription factor activity, RNA polymerase II cis-regulatory region sequence-specific DNA binding, DNA-binding transcription factor activity, RNA polymerase II-specific within cytosol, nucleus, nucleoplasm, Golgi apparatus to regulate cell differentiation, mammary gland involution, blastocyst development, negative regulation of DNA-templated transcription.4. The ETS domain within the designed protein should be capable of recognizing and binding to a range of DNA sequences. The designed protein sequence is ",
    "response#1": "GLHEKELWKKFHEAGTEMIITKAGRRMFPSYKVKVTGMNPKTKYILLIDIVPADDHRYKFCDNKWMVAGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNAFGSKNTAFCTHVFPETSFISVTSYQNHKITQLKIENNPFAKGFHVLQVHPDSPATGAHWMRQLVSGTLHEKELWKKFHEAGTEMIITKAGRRMFPSYKVKVTGMNPKTKYILLIDIVPADDHRYKFCDNKWMVAGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNAFGSKNTAFCTHVFPETSFISVTSYQNHKITQLKIENNPFAKGFRMEDGWEFKLADPDEVARRWGLTGLHEKELWKKFHEAGTEMIITKAGRRMFPSYKVKVTGMNPKTKYILLIDIVPADDHRYKFCDNKWMVAGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNAFGSKNTAFCTHVFPETSFISVTSYQNHKITQLKIENNPFAKGFHVLQGCERSSPAALHEKELWKKFHEAGTEMIITKAGRRMFPSYKVKVTGMNPKTKYILLIDIVPADDHRYKFCDNKWMVAGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNAFGSKNTAFCTHVFPETSFISVTSYQNHKITQLKIENNPFAKGFR",
    "response#2": "LHEKELWKKFHEAGTEMIITKAGRRMFPSYKVKVTGMNPKTKYILLIDIVPADDHRYKFCDNKWMVAGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNAFGSKNTAFCTHVFPETSFISVTSYQNHKITQLKIENNPFAKGFRLHEKELWKKFHEAGTEMIITKAGRRMFPSYKVKVTGMNPKTKYILLIDIVPADDHRYKFCDNKWMVAGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNAFGSKNTAFCTHVFPETSFISVTSYQNHKITQLKIENNPFAKGFRIRLYQFLLDLLRNGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSSLSRNLHEKELWKKFHEAGTEMIITKAGRRMFPSYKVKVTGMNPKTKYILLIDIVPADDHRYKFCDNKWMVAGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNAFGSKNTAFCTHVFPETSFISVTSYQNHKITQLKIENNPFAKGFRLQFSLVDTGLHEKELWKKFHEAGTEMIITKAGRRMFPSYKVKVTGMNPKTKYILLIDIVPADDHRYKFCDNKWMVAGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNAFGSKNTAFCTHVFPETSFISVTSYQNHKITQLKIENNPFAKGF",
    "response#3": "GLHEKELWKKFHEAGTEMIITKAGRRMFPSYKVKVTGMNPKTKYILLIDIVPADDHRYKFCDNKWMVAGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNAFGSKNTAFCTHVFPETSFISVTSYQNHKITQLKIENNPFAKGFISLPKGGTLEVLHEKELWKKFHEAGTEMIITKAGRRMFPSYKVKVTGMNPKTKYILLIDIVPADDHRYKFCDNKWMVAGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNAFGSKNTAFCTHVFPETSFISVTSYQNHKITQLKIENNPFAKGFRIRLYQFLLDLLRNGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFFVLDGTQVDAVSVNYCTVNQDFTRSNLNLLIDNSGKLREHESSESGAESYESSDSMLQSWNSQSSLVDLQRVPSYESFEDDCSQSLCMSKPTMSFKDYIQDRSDPVEQGKPVIPAAILAGFTP"
  },
  {
    "reference": "MVWALPRTSSPSCIAPSYKPDSGWIKMSAEWLIDWSCLLNGLRDLIAGCIQAVRDCNSFALTTVICLLMLFAWYCYRVGKDQPRSPFATVNLLIQSSEAKGLQNGFAYCHSRECVRCTHNDGLNQKLYHNLQEYAKRYSWSGMGRIHKGIREQGRYLNNRPSIQKPEVFFLPDLPTMPYFPRDAQKHDVELLEQNFATILSEFEAIYKAFSNCSLPQGWKVNSTPSGEWFTFYLVNQGVTIPSNCKKCPRTYRLLGNLRTFIGNNVFGNACISVLTPGTVITEHYGPTNIRIRCHLGLRIPGNCELVVGGEPQCWAEGHCLLFDDSFLHTAFHEGSAEEGPRVIFMVDLWHPNVAAAERQALDSIFAPGR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein must exhibit the following characteristics: May function as 2-oxoglutarate-dependent dioxygenase..2. The protein must be engineered to have a high affinity for Fe cation in order to increase its catalytic activity.3. The designed protein should have a high specificity for 2-oxoglutarate binding, with minimal off-target effects.4. The Lumenal, Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.5. The protein should have a Helical domain that is long enough to span the entire membrane.6. The designed protein should be involved in peptidyl-amino acid modification by localizing to membrane and exhibiting dioxygenase activity. The designed protein sequence is ",
    "response#1": "MILQISNAVDTDTVKSIVAGLDAGQFSDGKKTAGWAAKDVKNNQQLSGKKSEAATQVLLDRLQQNALVQSVMRPKQVARVTINRYQQGEYYGTHMDDSLMNGVRTDISFTLGLSPLSDFEGGELVIEDASGERSWRLGQGDILMYPSHYLHRVNPVTKGSRLAMIGWVK",
    "response#2": "MLIHVRDVLSAAELQQTRELLSDAPWADGGVTAGVQSAQVKNNQQLPQAHPVTQALQQLVMAGLNRHPTFFSAALPKKVFPPLFNRYGGTANAFGNHVDNAVRFITSGPHTGQRVRTDVSCTLFLAEPETYDGGELVIEDTYGSQRVKLAAGDLVLYPGTSVHRVEPVTRGARVGCFFWIRT",
    "response#3": "MLITIPDLLTPEEVAYMRQILEGTAWVDGRSTAGDQAGKVKRNLQVPVDSPEARELGNIVLRALGRSPVYSSAALPAHILPPMFNRYDEGMTFGAHVDGSIRVVPGTGQRIRTDVSTTVFLTPPEDYDGGELVMHDTYGQHSVKLPAGHAVVYPATSLHSVTPVTRGSRWASFFWAA"
  },
  {
    "reference": "MFTQIALILSLIILIFFIYKFAMFKPHVVLTPLEYFNSRKRKILEYLDNYKVKLNVSIPPLTCTDLDKNYDIEEFEVMKQTNPEKFKKYISCGYVHNVYLLTAFEEWINNSLTHSDVSFENFQYLCIRPPADISTVDFKNFKSYIKSCIQTNKTNVTYY",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "MT",
    "response#2": "MF",
    "response#3": "MPLLDSQGRVINTWADIINR"
  },
  {
    "reference": "MNIGIVGATGQVGAVMREILSQRNFPFESIRFFASSRSAGKEIEFQGQSIRVEDAAKAKWDNLDVVLFSAGGQLSKELAPKVASAGAIVIDNSSAWRMDPEVPLVVPEVNAHAINNMPKNIIANPNCTTMVAMPVLKPLHLEAGLRNLIVSTYQATSGAGLSGVQELNQQVSEAGDTAMDLTHDGQAIEFTPGNSFPEPIAFNVVPFCGSLIEDELQETNEERKLRDESRKILEIDDLAVTATCVRVPVYTGHSLAITAGFENHISSQRVEDILREVPAVVLEDVPTPLKSAGVDPTFVGRIRPDTVLENGINLFLSGDNLRKGAALNAIQIAETFLG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The Acyl-thioester intermediate active site of the designed protein must be optimized for substrate binding and specificity.2. The protein must exhibit NAD binding, aspartate-semialdehyde dehydrogenase activity, NADP binding.3. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..4. The presence of Semialdehyde dehydrogenase NAD-binding domains is critical for the interaction of the designed protein with its intended targets.5. The NADP(+), substrate, phosphate binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "NVAIVGATGIVGKTVLEILEERNFPVKNLYLLASARSVGDIREFKGKNYTVENLAKFDFSKTQLCFFCASNEIAAEYAPKAAALGNVVIDKSSHFRYDKDIPLVVPEVNGSIVLGLKPLHEAATIERINVATYQAVSGSGKEAIDELATQTANLLNGKPVECEAYPKQIAFNCLPHIDVFLDNGYTKEEMKMVWETQKIFGDDSILVNPTAVRVPVFFGHSEAVHIETREKLTEAKARELLSQFEGIEVLDDREPGGYPTAVTEAATHDPVYVGRIREDISHPRGLNLWVVADNVRKGLIGDLKPIYDAVGVTRINIATYQSVSGSGKEAVDELALQSAQILNGQPIHPKVYLQQIAFNVIPHIDAFLENGFTKEEMKVVWETQKILNDTNISINPTAVRVPVFYGHSAAVHIETRDKITAQQAEALLAKAKGVTLIQGKYPYPTPAKDVAGQDSVYVGRVREDLSHAKGLNLWVVADNVRKGLIGDATKAVLNVAIVGATGIVGKTVLEILEERNFPVKNLYLLASARSVGDIREFKGKNYTVENLAKFDFSKTQLCFFCASNEIAAEYAPKAAALGNVVIDKSSHFRYDKDIPLVVPEVNGSIVLGLKPLYDCARIKRVVVSTYQAVSGTGQKAIVELEDQVKSWAEGRTLEPKVYPHQIAFNCLPHIDVFMDNGYTREEMKMVNETRKIMEDPEIQVSATAVRVPVFYSHSEAVNVKFEQKISASKARELLTNAPGIKVIDDPFRNAYPLALEAEGQDLTLVGRIREDISQANGLDFWVVADNIR",
    "response#2": "LKPLYNLSPIKRVVVSTYQAVSGAGREGMDELKSQLDSLARGEAVTAKVLPVAKLDKHYQIASNLLPQIDVFMDNRYTKEEMKMVDETRKIMEDDALRVTATTIRVPVYRSHAESVNVEFADDVSVEAARTALENFPGVIVRDNPAEMIYPQPLETSGKDAVEVGRIRKDFSIEHGLNLWVCGDQIRGITDKTGLYLKPLHEAATIERINVATYQAVSGSGKEAIDELATQTANLLNGKPVECEAYPKQIAFNCLPHIDVFLDNGYTKEEMKMVWETQKIFGDDSILVNPTAVRVPVFFGHSEAVHIETREKLTEAKARELLSQFEGIEVLDDREPGGYPTAVTEAATHDPVYVGRIREDISHPRGLNLWVVADNVRDFESLKPIQDAVGIERINVCTYQSVSGAGKSAMEELAKQTADLLSSREITPEAFSRQIAFNAIPQIDVFMDNDYTKEEMKMSWETKKIMGDDTILVNPTAVRVPVFFGHGEAIHIETRQPIDAEEVKQLLANAPGVKVYETREEFPTQVSSASGNDEVHVGRIRNDITHPNGINMWVVSDNIRDFESEEYQANFGL",
    "response#3": "MLAVAGATGAVGIEILKCLEQRDFPISELTLLASARSAGKTISFRGKEVTVKELTPDSFAGVDIALFSAGGSISKAFASHAVAAGAVVVDNSSAFRMDENVPLVVPEINPEDVEAASPPKGIIANPNCTTMAAMPALYPLHRAFGVKRIFASSYQAVSGTGAKAIEELERQVGEVVAGKPVTREVYPHQIAFNVLPHVDSFLPSGYTKEEMKMENEGRKIMHHPGFRASVTCVRVPVYRAHSVAVSAEFEKPVTTAAALEVLRKAPGLDVVDDPSKNTYPLPLNAAEQYNCHVGRLRMDCALENGLCFWVVGDQLLKGAALNAVQVAELVAAKL"
  },
  {
    "reference": "MRVRGMQRNWQHLGKWGLLFLGILIICNARENLWVTVYYGVPVWKEATTTLFCASDAKSYEKEVHNVWATHACVPTDPNPQEVDLVNVTENFNIWKNNMVEQMHTDIISLWDQSLKPCAKLTPLCVTLNCVDATPNGTQSSTTATPTPDTLKEEKGYIKNCSFNMTTEVRDKQLKVHALFYRLDIVQISNSSNSSSGEYRLINCNTSTITQTCPKVSWDPIPIHYCAPAGYAILKYKRFNGTGPCRNVSTVQCTHGIKPVVSTQLLLNGSLAKEEIIIRSQNISDNAKTIIVHLNESVQINCTRPNNNTRKSIHLGPGQAFYATGDIIGDIRKAHCNISEAQWNKTVEQVKTKLRSYFTNTTTIIFKSPSGGDLEITTHSFNCRGEFFYCNTSELFTDKKFNATHGTITLPCRIKQIVNLWQEVGRAIYAAPIEGNITCNSNITGLLLTRDGGQNSSANETFRPGGGDMRDNWRSELYKYKVVEIEPLGIAPTKAKRRVVQREKRAVGIGALFIGFLGAAGSTMGAASITLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGLWGCSGKLICTTNVPWNYSWSNKSQDEIWGNMTWMEWEREISNYSNTIYKLIEDSQIQQERNEQELLALDKWTSLWNWFDISKWLWYIRIFIMVVGGLIGLRIVFAVLSIVNRVRKGYSPLSLQTHIPSPRGPDRPEGIEEGGGEQDRDRSVRLVSGFLALVWDDLRNLCLFSYRHLRDL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.2. The designed protein should contain at least one YXXL motif; contains endocytosis signal motif to achieve its intended function.3. For general function, the protein need meet that Transmembrane protein gp41: Acts as a class I viral fusion protein; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes4. Design the protein with a signal peptide incorporated.5. The designed protein's functionality will benefit from Basic and acidic residues compositional bias in its structure.6. The protein contains novel Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains that confer a unique function or activity.7. The protein should have a specific function in fusion of virus membrane with host plasma membrane, clathrin-dependent endocytosis of virus by host cell and should be localized to host cell endosome membrane, viral envelope, virion membrane in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "MRVKGIRNNYLWRWGTMLLGMLMICSATEQLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPADPNPQELELKNVTENFNMWKNDMVNQMHEDVISLWDQSLKPCVKLTPLCVTLNCSNVSSNSSGTSTANSTSSTTGGGEMKNCSFNITTSIGNKVKDRAFFNELDIEPLNNSTDNTRYRLRHCNTTVITQACPKVSFEPIPIHYCTPAGFAILKCNNQTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEKEVVIRSSNFSDNAKVIIVQLKNLVKINCTRPNNNTRKGIHLGPGRAWYATDIIGDIRQAHCNLSGADWNNTLAEVSKKLREKFGNKTIIFNQTSGGDPEITMHSFNCRGEFFYCDTSRLFNSTWSNNGTTNGTTGNDTITLPCRIRQIVNMWQEVGRAMYAPPIAGQINCSSNITGLLLTRDGGNTNDTNNSTETFRPGGGDMRDNWRSELYKYKIVKIEPLGIAPTKAKRRVVQREKRAIGMLGAMFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGLWGCSGKLICTTNVPWNSSWSKKNLTDIWDNMTWMQWEREIDNYTDTIYRLLEVSQTQQEQNEKDLLALDSWKNLWSWFSISKWLWYIKIFIMIVGGLIGLRIIFAVISIVRRVRQGYSPLSFQTLLPSPRGLDRPGRIEEE",
    "response#2": "MRVMGILRNCQQWWIWGILGFWMLMICRVMGNKWVTVYYGVPVWREAKTTLFCASDARAYEKEAHNVWATHACVPTDPDPQEVPLENVTENFNMWKNNMVDQMQEDIISLWDQSLKPCVKLTPLCVTLNCTNLNVTNSNSTSSNSTNSNSTSSNSTVGKRDEEMKNCSFNVTTNINNKIHKEYALFYKLDIMPIDNDNTSYTLISCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCRDKKYNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEDVVIKSQKFSDNAKNIIVQLNETVEINCTRPGNNTRKSIHMGPGKAFYATGDIIGDIRQAHCNLSSTKWNNTLKQIVNKLREQDIIGNSTINFKPPSGGDPEVVMHIFNCGGEFFYCNTSQLFNSTWENSAGENNTESNATEVNIILPCRIKQIINRWQEVGKAMYAPPIRGNISCSSNITGLILTRDGGNENRTGNETFRPGGGDMKDNWRSELYKYKVVKIEPLGIAPTKARRRVVQREKRAVGIGAVFLGFLGAAGSTGAKSMTLTVQARQLLSGIVQQQSNLLRAIEAQQQMLRLTVWGIKQLQARVLALERYLRDQQLLGIWGCSGKLICPTAVPWNSIWSNKSYNEIWDNMTWLQWDKKINNYTRLIYTLIEESQNQQEKNEQDLLALDKWASLWTWFDISNWLWYIRIFIMIVGGLIGLRIVFAVLSIVNRVRQGYSPLSFQTLTPSPREPDRLGRIEE",
    "response#3": "MRVRGIQRNWPQWWIWGILGFWMIIICKVVGNLWVTVYYGVPVWEDAETPLFCASDAKAYSTEKHNVWATHACVPTDPNPQERDLGNVTENFNMWKNNMVEQMHEDIISLWDESLKPCVKLTPLCVTLNCTDRAFSNNNSNSTSSNSTVGKRDEEMKNCSFNVTTNINNKIHKEYALFYKLDIMPIDNDNTSYTLISCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCRDKKYNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEDVVIKSQKFSDNAKNIIVQLNETVEINCTRPGNNTRKSIHMGPGKAFYATGDIIGDIRQAHCNLSSTKWNNTLKQIVNKLREQDIIGNSTINFKPPSGGDPEVVMHIFNCGGEFFYCNTSQLFNSTWENSAGENNTESNATEVNIILPCRIKQIINRWQEVGKAMYAPPIRGNISCSSNITGLILTRDGGNENRTGNETFRPGGGDMKDNWRSELYKYKVVKIEPLGIAPTKARRRVVQREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARQLLFGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGLWGCSGKLICTTNVPWNSSWSNKSQTDIWDNMTWMQWDREISNYSHTIYRLLEESQIQQEKNEKDLLALDSWNNLWNWFSITNWLWYIKIFIMIVGGLIGLRIIFSVLSIVNRVRQGYSPLSFQTLIPNPRGPDRLGRI"
  },
  {
    "reference": "MSPRVLGGLAAFLCLVLDQANKLWLIHVFDIEARRPVRLAPFFDIIYERNPGISYSLFRAQSAMGRWILVALTLFAILLLSIWLWRATNRLVALALGCIIGGALGNAIDRIAAGAVADFYYFHIGSFSWYVFNLADAAIVAGVALLILDAFTSEEAGVPAPDSEGHS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. We require a protein that can specifically bind to the metabolites produced in the lipoprotein biosynthesis (signal peptide cleavage) pathway.2. The designed protein must be able to perform aspartic-type endopeptidase activity under specific conditions.3. For general function, the protein need meet that This protein specifically catalyzes the removal of signal peptides from prolipoproteins. The designed protein sequence is ",
    "response#1": "ECNDS",
    "response#2": "LPGGAV",
    "response#3": "MGAALN"
  },
  {
    "reference": "MINYLRGQAIEVIKTPNNRLILILDVNQIGYEIQIPSRLALDIGNNNNDSCQIFTHLLLREEQPLLYGFGTAPERELFRQLLSVNGVGAQLALALIDTLGIEELVVAIVTGNTKILSKTPGVGLKTAERIALELKTKLAAWRQLREATTTITAILPAAAILEDVQMTLLALGYSQEEIDRAMAVLSQDALFSKNTQPEDWIKGAINWLG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein design must be able to perform DNA binding in order to contribute to DNA repair. The designed protein sequence is ",
    "response#1": "SSCKPLDELLGGGFESGVVTQIFGEAGSGKTNICLQLAIECVKKGKKAIFIDTEGLSADRFKQIAGENARKIAQDIIIFEPHSFEEQYSAVRETEKISTENVGVIIVDSATAYYRFELDDEDSSIRTRRELSNQIGFLHSLARKRDIVVVITNQVYSDIKSNSLKPIGGSSLEHISKTIIQLEKTGTGSRRAKIWKHRSRPEGTTCEFTITADGC",
    "response#2": "SSCKPLDELLGGGFESGVVTQIFGEAGSGKTNICLQLAIECVKKGKKAIFIDTEGLSADRFKQIAGENARKIAQDIIIFEPHSFEEQYSAVRETEKISTENVGVIIVDSATAYYRFELDDEDSSIRTRRELSNQIGFLHSLARKRDIVVVITNQVYSDIKSNSLKPIGGSSLEHISKTIIQLEKTGTGSRRAKIWKHRSRPEGTTCEFTITADGC",
    "response#3": "MGVNLKEIVDPVKKEIEFKQLFGKVIAIDAFNALYQFLFSIRQDGEPLRDSKGRITSHLSGLFYRTINLLEYGIKPIYVFDGTPPKFKIVAWEKRKKHKEQRR"
  },
  {
    "reference": "MKRKICKALICAALATSLWAGASTKVYAWDGKIDGTGTHAMIVTQGVSILENDLSKNEPESVRKNLEILKENMHELQLGSTYPDYDKNAYDLYQDHFWDPDTDNNFSKDNSWYLAYSIPDTGESQIRKFSALARYEWQRGNYKQATFYLGEAMHYFGDIDTPYHPANVTAVDSAGHVKFETFAEERKEQYKINTAGCKTNEAFYTDILKNKDFNAWSKEYARGFAKTGKSIYYSHASMSHSWDDWDYAAKVTLANSQKGTAGYIYRFLHDVSEGNDPSVGKNVKELVAYISTSGEKDAGTDDYMYFGIKTKDGKTQEWEMDNPGNDFMTGSKDTYTFKLKDENLKIDDIQNMWIRKRKYTAFSDAYKPENIKIIANGKVVVDKDINEWISGNSTYNIK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase C, leading to activation of eukaryotic phospholipases and further membrane damage.2. The designed protein have PLAT domains with specific sequence motifs that are crucial for its activity.3. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.4. The protein must be able to hydrolase activity.5. The designed protein should have a binding site that is specific to the target Zn(2+) ligand. The designed protein sequence is ",
    "response#1": "MKKKFLKGLCCAFVISITCLGASSKAYGWDGKKDGTGTHSMIVTQAVKVLENDMSKDEPEIVKQNFKILQDNMHKFQLGSTYPDYDPNAYKLFQDHFWDPDTDHNFSKDNLWYLSYSIKDTAESQVRKFTALARNEWEKGNYEKATWYFGQAMHYFGDLNTPYHAANVTAVDSIGHTKYEGFAEKRKDQYRINTTGIKTNEGFYADALKNSNFDSWSKEYCKGWAKQAKNLYYSHSTMKHTNEDWDYSASHALKNAQMGTAGCIYRFLYDVSKDLLPKN",
    "response#2": "MKKKFLKGLCCAFVISITCLGASSKAYGWDGKKDGTGTHSMIVTQAVKVLENDMSKDEPEIVKQNFKILQDNMHKFQLGSTYPDYDPNAYKLFQDHFWDPDTDHNFSKDNLWYLSYSIKDTAESQVRKFTALARNEWEKGNYEKATWYFGQAMHYFGDLNTPYHAANVTAVDSIGHTKYEGFAEKRKDQYRINTTGIKTNEGFYADALKNSNFDSWSKEYCKGWAKQAKNLYYSHSTMKHTNEDWDYSASHALKNAQMGTAGCIYRFLYDVSKDLLPA",
    "response#3": "MKKKFLKGLCCAFVISITCLGASSKAYGWDGKKDGTGTHSMIVTQAVKVLENDMSKDEPEIVKQNFKILQDNMHKFQLGSTYPDYDPNAYKLFQDHFWDPDTDHNFSKDNLWYLSYSIKDTAESQVRKFTALARNEWEKGNYEKATWYFGQAMHYFGDLNTPYHAANVTAVDSIGHTKYEGFAEKRKDQYRINTTGIKTNEGFYADALKNSNFDSWSKEYCKGWAKQAKNLYYSHSTMKHTNEDWDYSASHALKNAQMGTAGCIYRFLYDVSKDLLPK"
  },
  {
    "reference": "MSNNKKLTSLFGAPVSDRENSMTAGPRGPLVMQDWYFLEQMAHFDREVIPERRMHAKGSGAFGTFTVTNDITQYTSASIFSEVGKQTEMFARFSTVAGERGAADAERDIRGFALKFYTDEGNWDLVGNNTPVFFFRDPKLFASLNHAVKRDPRTNMRSAQNNWDFWTSLPEALHQVTILMSDRGIPKGYRNMHGFGSHTYSMYNDKGERVWVKFHHRTQQGIENLQPDEAAKIIADDRESSQRDLFEAIENKDYPKWKTYIQVMTEEQARNHKDNPFDLTKVWYKGDYPLIEVGEWELNRNPDNYFQDVEQAAFAPTNIVPGIDFSPDKMLQGRLFSYGDAQRYRLGVNHWQIPVNQPKGVGVENICPFSRDGQMRILDNNQGGGTHYYPNSDGSFEDQPEFKKPGLKVEGEAYEYDFRQDDDNYFEQPGRLFRLQSKEQQERIFENTANEMQGTTLEVQHRHIRHCYKADSEYGKGVARALGVDINDVDLEIKD",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics.3. A protein with heme binding, metal ion binding, catalase activity is needed to enhance hydrogen peroxide catabolic process, response to oxidative stress in a specific context.4. The designed protein should have a high specificity for heme binding, with minimal off-target effects.5. The designed protein is the optimization of the active site to achieve high catalytic efficiency for 2 H2O2 = 2 H2O + O2.6. The presence of heme is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "TFARAWFKLLHRDMGPKTRYLGPEVPKEDLIWQDPIPEGNKDFNISKAKELIKSADLTIQELIETAWASASTYRHTDMRGGANGARIMLSPQKDWDVNKPDQLRKVLNIYKDIAKKVNSSTADIIVLAGNVGIEKATGVDVPFIAGRGDALQEQTDIESFSVLEPMADGFRNFQKRDFSVSPEEMLLDKSQLIGLTAPEMTVLIGGFRSLGISSSGNEGVFTNDINTLSNDFFIDLLDMSIEWKPASKNTYDGFKRGTSEKVKTASRVDLVFGSNSQLRAIAEVYAT",
    "response#2": "TFARAWFKLLHRDMGPKTRYLGPEVPKEDLIWQDPIPEGNKDFNISKAKELIKSADLTIQELIETAWASASTYRHTDMRGGANGARIMLSPQKDWDVNKPDQLRKVLNIYKDIAKKVNSSTADIIVLAGNVGIEKATGVDVPFIAGRGDALQEQTDIESFSVLEPMADGFRNFQKRDFSVSPEEMLLDKSQLIGLTAPEMTVLIGGFRSLGISSSGNEGVFTNDINTLSNDFFIDLLDMSIEWKPASKNTYDGFKRGTSEKVKTASRVDLVFGSNSQLRAIAEVYAR",
    "response#3": "TFARAWFKLLHRDMGPKTRYLGPEVPKEDLIWQDPIPEGNKDFNISKAKELIKSADLTIQELIETAWASASTYRHTDMRGGANGARIMLSPQKDWDVNKPDQLRKVLNIYKDIAKKVNSSTADIIVLAGNVGIEKATGVDVPFIAGRGDALQEQTDIESFSVLEPMADGFRNFQKRDFSVSPEEMLLDKSQLIGLTAPEMTVLIGGFRSLGISSSGNEGVFTNDINTLSNDFFIDLLDMSIEWKPASKNTYDGFKRGTSEKVKTASRVDLVFGSNSQLRAIAEVYAV"
  },
  {
    "reference": "MSELSLRAKKLDAADELAPLRSRFVLDNASDHAADAAGIYLDGNSLGALQANVPGRIEDVVRRQWGELRIRSWDESGWWTAPERIGDRIAPLVGAAPGQIVVGDSTSVNVFKALVAAVRMVDPTRDEILVDATTFPTDGYIAESAARLTGRTLRPVTPAEVPAALGERTAAVLLNHVDYRSGRLHDLPSLTAAIHASGALAVWDLCHSVGALPVGLDEHGVDLAVGCTYKYLNGGPGAPAFLYVRRDLQDRFDSPLPGWNSHAEPFGMSQSYDPAPGALRGRVGTPDILSMLALEAALEIWDEVSVAAVRAKSLALTDFFLECVAAYTEPGRVTSVTPELHEERGSQIALRCPDAGDVMKQLIDRGVVGDFRNPDVLRFGFSPLYVSFAETERAARVLAEVLG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The pyridoxal 5'-phosphate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "ITELIGNTPLLRLKNFDVPEGVAVYAKLEMMNPGGSIKDRLGDMLIRDALDSGKVKPGGVIIEATAGNTGIGLALSARKYGLKAIFCVPEHFSREKQQIMQALGASIIHTPRQDGMQGAIQKAIQLETEIENSYCVLQFKNRVNPSTYYKTLGPEMWEALDGNIHTFVAGAGSGGTFAGTASFLKEKNPAVKTVIVEPVGSILNGGEPHAHKTEGIGMEFIPDYMDKSHFDEIYTVTDENAFRLVKEAAEKEGLLIGSSSGAALYAALEEAKKASAGTNIVTVFPR",
    "response#2": "ITELIGNTPLLRLKNFDVPEGVAVYAKLEMMNPGGSIKDRLGDMLIRDALDSGKVKPGGVIIEATAGNTGIGLALSARKYGLKAIFCVPEHFSREKQQIMQALGASIIHTPRQDGMQGAIQKAIQLETEIENSYCVLQFKNRVNPSTYYKTLGPEMWEALDGNIHTFVAGAGSGGTFAGTASFLKEKNPAVKTVIVEPVGSILNGGEPHAHKTEGIGMEFIPDYMDKSHFDEIYTVTDENAFRLVKEAAEKEGLLIGSSSGAALYAALEEAKKASAGTNIVTVFPR",
    "response#3": "HKTRLQIAEDPSLDYEYLPLVGLKSFIQSSLELLFGKHSEAIAEKRVGGVHIVGESGAFQLGAQFLKTWRKNVKIVCIVSCQKEQCGLIFQDMGFIVYEYSIWNASDLCSDPSMFVEVLQHIPVGSILVIGNITDCKFTQNQWTKLMSIIKSKQIFPFFDIPCQGLSTGDLEEDTKILQYFVSLGLEFFCSQSLSKNFGIYDEGVGILVVAALSNQHLLCVLSQLMDYVQALWGNPPATGARIITSILCNPALFGEWKQSLKGVVENMMLIKEKVKEKLRLLGTPGSWDHITRQSGTHGYLGLTYQQVEFLVKKKHIYLPKTSRINFTCINARNIDYITQSV"
  },
  {
    "reference": "MTGGTLNFITFNQDYSCLAVGTSKGFRFYHTDPFSKIFSSDEGNVSIIEMLFSTSLVALILTPRQLEIQNTKRASVICELTFPSAVLAVRLNRKRLAVVLECQIYLYDVSNMNLVQTIDTSPNPNAICALSPSSDSCYLVYPRPNPREDVGAKAPAHLPPPSQYVPPKRGDVLVYDALNLKTVNVVEAHKSPLCAIALNHDGSMLATASETGTIIRVFSLPQGQKLFQFRRGTVPTSIYSMSFNLSSTLLCVSSTSDTVHIFRLLNTQKNANALPGGGPAQGTRSRSFDVNDPSPHKGEVPSAGNGNNGSGSHKREPSGSFGNMLRRSSQMMVRGVAGVASSYLPQSVAEMWEPERDFAFIKIPKSSNNRAGGLSATPLRSVVAMSSSSPQVMVVTSDGGFYVYNIDMEKGGEGYLVKQFSVLDGDDKLTNSDYGA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. A protein that can perform phosphatidylinositol-3,5-bisphosphate binding, phosphatidylinositol-3-phosphate binding in endosome membrane to support protein transport.2. The protein must exhibit the following characteristics: Required for cytoplasm to vacuole transport (Cvt) vesicle formation, pexophagy and starvation- induced autophagy.3. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "LNCKNHNVLSNYNVANKDILLKNELDTPPSKTSEMWYLAPCEDGSRRQRPSECSSDDLMCAIRHVKVDGSDHLTQKLDVHKSAKYEVEEVESGGFDIRFLGVKWGSNTIDATLSYSCDTNLKTDELVTTIWNSEYVLIEMSGPSGCKA",
    "response#2": "RKLLEMRGAFIIRQLCLLLHAENIFHSMADILLKEEDLKFASTMVQTLNTILLTSAELFQLRNQLKDLRTQESCALFCCLYRSWCHNPVATVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLMEVDKLVQLIESPIFTYLRLQLLDVEHNPYLIKALYGLLMLLPQSQAFQLLSHRLSCR",
    "response#3": "VDTLCAMWESWNARVFHSLDTEGIKQAQCYEASAYRLFLVRCVQKKNISKCNEFFRKMSSLTLNNPQWADWFAFPYNHHAKDTEPFRKYFDKTWIEIYYVSLHNFLSTSC"
  },
  {
    "reference": "MNWQDPLVKKFLYLIVAMVILCPLGILLVWNYGDAWGEWGPEDVAEKVGEDKVSGLLHLADIWSYAPLPDYDIPGWDDPFHASIGYIISAIVGVILCVGAYYALIKIVNPKAAAG",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be expressed specifically in the plasma membrane The designed protein sequence is ",
    "response#1": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPX",
    "response#2": "AFHDHILMPIITAITLLVLGLLIWIVIRYNAKANPVPAKFSHNTTVEIIWTIGPVLILMVIAIFSFRLLFAYNDMPKPYLTVKATGYQWYWGYEYPDQKISEITSVPLKQEEAVAKGVPYLLAADKPMVVPVNQVIRVQVTGADVIHAFALPAFGLKTDAIPGRLNETWFKADKIGVYYGQCSELCGVDHSNMPIEIHVVSQADFDAWA",
    "response#3": "AFHDHILMPIITAITLLVLGLLIWIVIRYNAKANPVPAKFSHNTTVEIIWTIGPVLILMVIAIFSFRLLFAYNDMPKPYLTVKATGYQWYWGYEYPDQKISEITSVPLKQEEAVAKGVPYLLAADKPMVVPVNQVIRVQVTGADVIHAFALPAFGLKTDAIPGRLNETWFKADKIGVYYGQCSELCGVDHSNMPIEIHVVSQADFDAWM"
  },
  {
    "reference": "MFVPETEDMLPRLAPRPSAAVPMGHTNEIIGPTVPEVSILFGQPPQDPQMQPQQPNHAHQESSPRAAPTFASWKKQMLPRVNFSPILATELGPRTAPSSNSPCSSKEYQVMPRDRNYASDAQQTTNHMNCNAGGYNAEPKQQAYQSRRLSSSSNADVLTICAGTQTDPFNPSPPRKSLPQVVYSDIDVSNLANKSDLAALVSLVESVRHEQQQLRNLCEMILEQQQRAKPEAISKASSTTGTQCDILTTNQAGRRLSPIMQDYIAEEEEPLPPPQARVIPQFPSPRPHPPIAQSTGYRANTPQAKRIPQLAKPNTEKSMVMNELALKYLRQPVDELMKDMRLGASPKSPNPEPLRPIDNIGHAQSPNDISNASYKYLKKYRLLPEEQLEPVCPQSPMAATAPSPHIQLDLENIRNQPKLL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a specific function within the centriole.2. Prioritize Polar residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "GRSRSKSRARSQSRGRDKSVKITVNSRNRARRQPGRDKRQSSQRVRNIVNKQLRKQGVTGPKPAICQRATATLGTVGSNTSGTTEIEACILLNPVLVKDATGSTQFGPVQALGAQYSMWKLKYLNVKLTSMVGASAVNGTVSGVSLNPTTTPTSTSWSGLGARKHLDVTVGKNATFKLKPSDLGGPRDGWWLTNTNDNASDTLGPSIEIHTLGRTMSSYKNEQFTGGLFLVELASEWCFTGC",
    "response#2": "GRSRSKSRARSQSRGRDKSVKITVNSRNRARRQPGRDKRQSSQRVRNIVNKQLRKQGVTGPKPAICQRATATLGTVGSNTSGTTEIEACILLNPVLVKDATGSTQFGPVQALGAQYSMWKLKYLNVKLTSMVGASAVNGTVSGVSLNPTTTPTSTSWSGLGARKHLDVTVGKNATFKLKPSDLGGPRDGWWLTNTNDNASDTLGPSIEIHTLGRTMSSYKNEQFTGGLFLVELASEWCFTGC",
    "response#3": "GRSRSKSRARSQSRGRDKSVKITVNSRNRARRQPGRDKRQSSQRVRNIVNKQLRKQGVTGPKPAICQRATATLGTVGSNTSGTTEIEACILLNPVLVKDATGSTQFGPVQALGAQYSMWKLKYLNVKLTSMVGASAVNGTVSGVSLNPTTTPTSTSWSGLGARKHLDVTVGKNATFKLKPSDLGGPRDGWWLTNTNDNASDTLGPSIEIHTLGRTMSSYKNEQFTGGLFLVELASEWCFTGC"
  },
  {
    "reference": "MNRHHDPNPFDEDEEIVNPFSKGGGRVPAASRPVEYGQSLDATVDIPLDNMNDSSQKQRKLADWEAELRKKEMDIKRREEAIAKFGVQIDDKNWPPFFPIIHHDIAKEIPVHAQKLQYLAFASWLGIVLCLVFNVIATMVCWIKGGGVKIFFLATIYALIGCPLSYVLWYRPLYRAMRTDSALKFGWFFFTYLIHIGFCIVAAIAPPIFFHGKSLTGVLAAIDVISDSLLAGIFYFIGFGLFCLESLLSLWVLQKIYLYFRGNK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "GSHSLRYFYTAVSRPGLGEPWFIIVGYVDDMQVLRFSSKEETPRMAPWLEQEEADNWEQQTRIVTIQGQLSERNLMTLVHFYNKSMDDSHTLQWLQGCDVEPDRHLCLWYNQLAYDSEDLPTLNENPSSCTVF",
    "response#2": "GSHSLRYFYTAVSRPGLGEPWFIIVGYVDDMQVLRFSSKEETPRMAPWLEQEEADNWEQQTRIVTIQGQLSERNLMTLVHFYNKSMDDSHTLQWLQGCDVEPDRHLCLWYNQLAYDSEDLPTLNENPSSCTVL",
    "response#3": "GSHSLRYFYTAVSRPGLGEPWFIIVGYVDDMQVLRFSSKEETPRMAPWLEQEEADNWEQQTRIVTIQGQLSERNLMTLVHFYNKSMDDSHTLQWLQGCDVEPDRHLCLWYNQLAYDSEDLPTLNENPSSCTVF"
  },
  {
    "reference": "MIYKSDTIQVELLDSKIATLCFNSDGSVNKFDRKTLASVSEALDVISATAEIRGLLLTSTKDNFIVGADITEFLELFAQEDKVLLSWLQLANSIFNKLEDLPYPTAAAVNGYALGGGCEVILATDFRIGDNSAKVGLPETKLGIIPGFGGTVRLPRLIGADNALEWITTGKEHRPDDALKVGFLDAVTTPENLQNVALQMLEDAISGKLDWQARRSQKQQALQLDKMEALMSFTTAKGMVFAVAGKHYPAPMAAVGVVEQAASMDREQALQVEHQAFIKLAKTDVAQALIGIFLNDQLVKSKAKKAIKTAHAVQHAAVLGAGIMGGGIAYQSASKGTPIVMKDINDNALTLGLSEAAKLLTAQVERGRSSPQKMAQVLNAITPALDYSAVKQADIVVEAVVENPKVKSAVLAEVEQVLADDAVIASNTSTISIDLLAQNLKQPQRFCGMHFFNPVHKMPLVEVIRGKDTSDSTIATVVAYASKMGKTPIVVNDCPGFFVNRVLFPYFAGFNALIADGADFAVVDKVMEKQFGWPMGPAYLLDVVGMDTGHHAQEVMAQGFPERMATSGQDALQLMFAQNRLGQKNGKGFYRYNPDKKGKPKKETDAITYELLAAAFGSLKEFSADEIIARTMIPMINETVRCLEEGIVASPAEADMGLVYGLGFPPFRGGVFRYVETMGLANYVAWADKYAHLGPLYQVTDAMRQLAAENGHYYQH",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. We require a protein that can specifically bind to the metabolites produced in the Lipid metabolism pathway.2. The NAD(+) binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "ARLVGAHPDEVAVMSSLTVNLHLLLASMYRPDRKRYKIMMESKAFPSDHYVVASQIKFHGYDPDTSIVHVAPREGQAVLLMEDILECIEKHGEEIALILFSGVQYYTGQLFDMKRITETGHAKGCIVGFDLAHAVGNVALKLHDWNVDFAAWCTYKYLNSGAGGIGGLFFHR",
    "response#2": "ARLVGAHPDEVAVMSSLTVNLHLLLASMYRPDRKRYKIMMESKAFPSDHYVVASQIKFHGYDPDTSIVHVAPREGQAVLLMEDILECIEKHGEEIALILFSGVQYYTGQLFDMKRITETGHAKGCIVGFDLAHAVGNVALKLHDWNVDFAAWCTYKYLNSGAGGIGGLFFHA",
    "response#3": "NLDSQRLQYIITSVLKVQTSGKEVWVVGEQGENKVCSWSGRDGVLSPSSQAQLSSRAMELLKVKGQRSWSVGLSVADLVDTIINNKRKVHSVSTLAKGYYGLDNEVFLSLPCILGTGGVSEVIKTG"
  },
  {
    "reference": "MRKLLFVTGGVCSSLGKGIAASSIGALMEARGFNVQMVKIDPYLNVDAGTMSPYQHGEVYVTDDGAETDLDLGNYGRFTTAKLSRSNSITTGQVYKKVIEKERAGDYLGRTVQVVPHITDEIKERILAVASNPDVDMTIVEIGGTVGDIESVPFLEACRQLIHELGKQNAISIHVTLVPAVSSGEIKTKPTQHAVKELQEIGIQPDALILRSSKPLSDELRRKISLFTNVEEEGVISGYDAATIYEVPLIYYGQRLDTFLLRKMNVESRHAELNKWQTVVQAITKPNRKVKIAVVGKYMDLQDAYKSIWEALIHGGIAYHAGIDIERIDSSRLEDPRADLDAIFKDIHGILIPGGFGERGIEGMICAARYAREKNIPYFGICLGMQIMVIEYARSILGWQDAHSTEFNKSTQHPVVCLLEEQKKMKNYGGTMRLGAYDAVTKVGSNIRAAYGVELISERHRHRYEVNSDIRLELEAHDLRISATTVDGELIEACEWPNHPWSVGVQFHPEFKSRPTAPHPLFREFIGAALSLSRL",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.2. The designed protein should be able to compete with endogenous proteins or inhibitors for L-glutamine, ATP, Mg(2+), CTP, UTP binding.3. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile; for glutamine hydrolysis, Nucleophile, active site to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).4. A protein with ATP binding, glutaminase activity functions is required to enable glutamine metabolic process, 'de novo' CTP biosynthetic process.5. The protein design should consider the endogenous proteins and enzymes present in the Pyrimidine metabolism, CTP from UDP: step 2/2, CTP biosynthesis via de novo pathway pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "KYVVVSGGVLSGIGKGVTASSIGVLLKSMGLRVTAIKIDPYLNSDAGTMSPFEHGEVFVLDDGGEVDLDLGNYERFLDIRLGRDNNLTTGKIYSGVIERERKGDYLGKTVQVVPHITNEIQEWIERVASQCSDGSGEEPDVCVIELGGTVGDIESAPFVEALRQFQFRVGRENICFFHVSLVPVIGVVGEQKTKPTQHTVKELRAAGLAPDFLVCRSSEPLYPEVKEKLSSFCHVEPSNVYSAHDVPNIYHVALMLNEQGASEKIVDLAKAYFSPKEDAYKSIAEALTHGGMANRVKVRIEWVDAELFDKEDATPYLQGFHAILVPGGFGERGTEGKIKAAQYAREHKVPYLGICLGMQMAVIEAARNVAGIEEAGSEEFDHEAGKKRFEPVVYHLKEWVQGNHKVSRSADDDKGGTMRLGAYDAALTEGSKVAEVYGSNAIEERHRHRYEVDIKYREQLEACGLKFTGMSPDGRLPEIVEWTDHPWFIGVQFHPELKSKPFDPHPLFKDFVLQDAYKSLNEALVHGGIANRVKVNIRWIDAEIFEHEDSDVAASLEPLHAILVPGAFGERGAEGKIASVKFARERQVPYFGICFGMQMACIEGARNLAGIEKASSTEFGPTDEPVVGIITEWMTAEGVEKREAGGDLGGTMRLGAYDAKLAGNSVVSSIYGATDISERHRHRYEVNTGYRTALEKGGLVFSGMSPDGMLPEIVERPDHPWFVGVQFHPELKSKPFDPHPLFASFIKKG",
    "response#2": "MTKYIFVTGGVSSSLGKGIIAASLAKLLQARGFRVTIQKLDPYINVDPGTLNPYEHGECYVTDDGAETDLDLGHYERFLNVKTSQANNVTTGRIYQSVIEKERRGEFLGKTVQVVPHITNEIKERVQLLGNNGDYDIVITEIGGTVGDIESLPYIEAVRQLRWELGDDNALVIHLTLVPYLSAAKELKTKPTQHSVKTLMESGIKADILVCRTEHELSDDLRTKLALFCNVKREAVIQSIDASTIYDVPNMMLREGLDTVTLKKLNLHDAYKSVYEALFHAGLAGQVAVELVKIDSSKLEEAASADSILGPEAAGGGVDGVLVPGGFGQRGINGMVKAAEWARVNKVPYLGICLGMQIMVIDWARGVLGWEDGDSSEFNPDTKHPVVSLLEEQVDVKNYGGTMRLGKSESAAEPGTRIFAAYGKAQIFERHRHRYEFSNKYRTEMAGSGLRISALTPDGSLVEAVEWPDHPWGVGVQFHPEFKSKPTAANPLFRDFIKSKLGG",
    "response#3": "LPDAYLSVTEALRAGAFATDTRVELRWITSDECSSPEETAALLDGVDGIIVPGGFGIRGIEGKLTALRHARENGLPTLGICLGLQCMVIEAARGLAGLAEANSTEFVSDTPHPVISTMADQHEVVAGTRDMGGTMRLGLYSCTLAPGTVARREYGQPEVAERHRHRYEVNNDYRDRLAAAGLVFSGTSPDGRLVEVVELPADVHPYYVGTQAHPEFRSRPTRAHPLFRGLPWQE"
  },
  {
    "reference": "MPKYVFVTGGVVSSVGKGVLAASLGLLLEHRGLRIAMMKFDPYINVDPGTMNPYEHGEVFVTDDGAETDLDLGHYQRFTHANLSQKSNVTTGGIYNSVIQKERRGEYLGKTVQVIPHITDEIKSRIRMLTTEENSDVDVAIIEIGGTVGDIESQPFLEAIRQFKLEVGARECCYVHVTLVPYIEAAGELKTKPTQHSVRALRDIGIQPNVIVCRTGSHKLGPDQRRKVAMFCDVSDNAIINAEDISPFYAIPLNLKKQGLDDTVLGLLELDAPEGDLSEWRAIVDRLVSARETVRIAAVGKYVSHHDAYKSINEAFVHAGIANDCKVQLEWVDSERFEQGEDPETVLAEYDGVLIGPGFGSRGIEGKIRAVRYVREQKKPFFGICLGMQIAVIEMTRTCLGRSKANSTEFDTETPDPVVSLLTEQLGIQAMGGTMRLGAYRCELKSGTKVRAAYGQDVISERHRHRYEFNNKYREPLEKSAGLIVSGIHPKTDHELVEIIELKDHPWFCASQFHPEFKSTPLNPQPLFRDFVKAALASRRKRG",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. A protein with ATP binding, CTP synthase activity is needed to enhance 'de novo' CTP biosynthetic process in a specific context.2. The designed protein should have a high specificity for L-glutamine binding, with minimal off-target effects.3. The desired protein should be able to perform H2O + L-glutamine = L-glutamate + NH4(+)4. The protein should be optimized for activity in CTP from UDP: step 2/2 pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "MVGKYMDLLDAYKSLNEALTHAGIHSETKVNIDFVDSERVEAEGVEILAGADAVLVPGGFGGRGFEGKILAAGYARRNKIPYLGICYGLHAAIIEFARDVAGLKGANSTEVDQNTPHPIIGLITEWVNEAGLTETRSLDSDLGGTMRMGEQECVLTEGSIARAVYGEARIKERHRHRFEVNPSYVSELEQAGLRIAGVSKDQSLVEVIEVVDHPWFVACQFHPEFTSTPRGGHPLFLNFL",
    "response#2": "MVGKYMDLLDAYKSLNEALTHAGIHSETKVNIDFVDSERVEAEGVEILAGADAVLVPGGFGGRGFEGKILAAGYARRNKIPYLGICYGLHAAIIEFARDVAGLKGANSTEVDQNTPHPIIGLITEWVNEAGLTETRSLDSDLGGTMRMGEQECVLTEGSIARAVYGEARIKERHRHRFEVNPSYVSELEQAGLRIAGVSKDQSLVEVIEVVDHPWFVACQFHPEFTSTPRGGHPLFLNFLG",
    "response#3": "MVGKYMDLLDAYKSLNEALTHAGIHSETKVNIDFVDSERVEAEGVEILAGADAVLVPGGFGGRGFEGKILAAGYARRNKIPYLGICYGLHAAIIEFARDVAGLKGANSTEVDQNTPHPIIGLITEWVNEAGLTETRSLDSDLGGTMRMGEQECVLTEGSIARAVYGEARIKERHRHRFEVNPSYVSELEQAGLRIAGVSKDQSLVEVIEVVDHPWFVACQFHPEFTSTPRGGHPLFLNFVK"
  },
  {
    "reference": "MRKFNVAVVGATGAVGEELFRVLEEYEFPINKLIPLASSRSAGSKIEYAGKEVTVLELTETVFEENDVEIAFFSAGGSISEKFAKFAVEAGAVVIDNTSHFRMDPKVPLVVPEVNPEDIKLWKETGIIANPNCSTIQMVQSLKPLDELYGIKRVDVSTYQAVSGAGKKGMEELVFQMQAFFAFNLEDAEKSAFPHQIALNVIPQIDVAQDNGFTKEEMKMVKETQKIMHKNIQVAATCVRVPVLRSHSESITVTFNEGVDVDLDAVRDALNNFENVKVIDDLENKKYPMPLIATDTDFTYVGRIKKDIYASNIIHYFNVADQVRVGAATNAVRIGLKWIEMENDI",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein must have at least one Semialdehyde dehydrogenase NAD-binding domain.2. The protein should have a high affinity for substrate, NADP(+), phosphate.3. The protein must exhibit the following characteristics: Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate..4. The design of the protein must focus on the precise arrangement of amino acids in the Acyl-thioester intermediate, Proton acceptor to enable effective catalysis of L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.5. The designed protein must possess aspartate-semialdehyde dehydrogenase activity, NAD binding, NADP binding, protein dimerization activity to support 'de novo' L-methionine biosynthetic process, diaminopimelate biosynthetic process, isoleucine biosynthetic process, threonine biosynthetic process under certain conditions.6. A protein should be able to manipulate the activity of enzymes in the L-threonine from L-aspartate: step 2/5, Amino-acid biosynthesis, L-threonine biosynthesis pathway, either by activating or inhibiting their function. The designed protein sequence is ",
    "response#1": "RVGVVGATGQVGAVMRKLLEERDFPADEVRFFASSRSAGKTLPWRGGEIVVEDTETADPSGLDIALFSAGATMSRVQAPRFAATGVTVIDNSSAWRKDPEVPLVVSEVNPEQTRNDALNHPQGIIANPNCTTMAAMPVLKPLHDIAKIRRVVVSTYQSVSGAGNAAMDELFSQTRAVFVNDDLTREHFTKQIAFNVIPHIDVFRDNGDTKEEWKMVVETQKMLDPAIRMNATCVRVPVFVGHSEAVNIEFETPVSEAEARAAFENAPGVLLVDRREDEGYVTPVECVGEYETFVSRLRVDPSVENGLSMWVVSDNLRKGAALNAVQIAELVASK",
    "response#2": "MSKVAVVGATGNVGREMLQTMVERDFPADKVVALAXHRSVGREVAYGDDVTLQCEDLATYDFKGTDIVLSSPGASVSAKFAPIAAAAGAVVIDNTSHFRMDPDVPLVIPEVNPEAIAGALARGVKTYVGGNCTVSLMLMALGGLLEHDLVEWISPMTYQAASGSGAQHMRELLRQMGHLHAAAGTRLADPAGAILDIDRDVSEALRSAEYPADQFGVPLAGSLIPWIDRAMDSGQSREEWKAGVETNKILGRRTNPLPIDGLCVRIGAMRSHAQALTIKLRRDVPMADIETLLAEANDWVHVVPNEPAASQQQLTPAAVSGGLDIPVGRLRKLAMGDDYLSAFTVGDQLLWGAAEPLRRMLRILL",
    "response#3": "MAKVAVCGATGAVGNQMLQCLEERDFPVGELRLLASERSRGKTLTYKGQSIPVQVLGPDSFQGIQIALFSAGASTSKQFAPLAARAGAVVVDNSSCWRMDPAVPLVVPEVNPQDVAADALNKGVKTYVGGNCTVSLMLMALEPLHRAFGIKRVDVSTYQAVSGAGKKGMEELVTQMQKFFDFSLEECEPKAFPHRIALNVIPHIDVFLGNDYTKEEMKMVNETNKIMHSDFAVSATCVRVPVLRSHSEAITIKFAREVSAKEAQEVLRKAESVVLIDNPQEKSYPMPIIATDRDETYVGRIRVDNYDKRILHLWCVADQIWGAAEPLRRMLRILLQRS"
  },
  {
    "reference": "MNKNKKRHRFDFLPNRLNKYSIRKFTNGIASVLIGSTILLGAVIDKEADAAEQQPTSEVYGQTDNNYKSESNKSNSVQHDEERTNIINDNDEANYSNHSEIPIHDKSSEYDQKPINEQDTSNHHETHLNQNATENHVKEESKEVSTEEESIEDRKTEESTTEESKAVEEANKENTTEKNDEGSLDLEKEKDTYKDEKDNGKKKNELESHNHRIENKVEDNVYKNNKETNLESKNENVNKDDKVNTSSSTSIEKPEDNATRSNLINSVNHSLKQLDNAKNNTEKQSLLENYYQTHTNATASDAKKAIEKLNIDFTKQNSDQLIALLLIELANQMDKDKVQANVPASKRAETNNESLSIETNTTNIEKTLAKPSTSKFRSANTRATNVVNYAANQSGRNVNHLVFANTSYEILGGGKKYNQVFMTMDGKLKIKIDYTVDDSVVEGDYFTVDFGKYIHPGTSRKPYRVNNIHDANGRTIAIGSYDSATNTAKYTFTNYVDIYNNVRGSFSLLSWPFKELVTTDKQSVPVGITVAGEDYTQNVIFNYGNRTVPVISDINYLTKDFAEFTTYINQNRAFNTGSKVRLSGQGFKFTSPDEIEVYKVLNNSQFRDSFSPDYANLTQVRNPKIIINSDGSATVDLGDIGTLGYIIRSKPNTLPDFSGIGVLKSEYTFTNNKNQRDTRAHASSIQFVRAELAGFGGFGGYVWFDKNNDGVQNDSNAAAAGITVNLLDPTGIRLATTTTDITGHYNFDNLTNGNYLVEFVMPEGYIPT",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Shows hemagglutination with sheep erythrocytes as well as good adherence to human T24 bladder carcinoma cells when overexpressed in a strain with non-hemagglutination.; Involved in adherence to the cells of the urinary tract2. The designed protein should contain at least one LPXTG sorting signal motif to achieve its intended function.3. Design the protein with a signal peptide incorporated.4. The protein should be optimized for stability and expression in extracellular region where it will participate in cell adhesion.5. The protein should exhibit Polar residues compositional bias.6. The protein design should prioritize the formation of helix-strand-helix-strand-turn-strand-strand-turn-strand-strand-turn-strand-helix-strand-turn elements. The designed protein sequence is ",
    "response#1": "MVGKDIRFTCSAASSSSSPMTFAWKKDNEVLTNADMENFVHVHAQDGEVMEYTTILHLRQVTFGHEGRYQCVITNHFR",
    "response#2": "ATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAPMADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSGLDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQVYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDSLQKKWVLIGVLSSGTNYGNNWVVTTQDFLGN",
    "response#3": "ATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAPMADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSGLDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQVYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDSLQKKWVLIGVLSSGTNYGNNWVVTTQDFLGTERPVVNVPSEITVYRGESFEYFATVTDNSNAFDLAKTVVRWLYNNQPGRGTEWLQYSVTQVGNQLKVRIFGNVPIDTTIGDYTRYVVATDAAGNVNATQF"
  },
  {
    "reference": "MFSHFFIQTKRIAVIGAGASGLPAIKCCLDEGLLPVCFELSESIGGLWNYTPEARDGRGTVMKSTVINVSKELMAFSDFPIPKEFPMFMHNTLVQKYLEMYAEHFNLLRHVQFQKEVVWCEPTVDFEKTGKWTLKIKDIKSGKIINEIFDGVMAATGHHTAPLLPAFDGQDIFKGRTLHTVQYRDSLEFSEKRVLIIGIGNSGADIACELSRVSSKVVLSTRSGSWVIQRIGDKGMPFDMLGTTRAVQYFQKCLPLSLLNSLTEMQINARFDHEKYGLKPNYPPRAQDPLISDEFHLRIANGLVQIRPNVQHFTGSSVVFVDGSVYDDIDVVIFATGYKIGFTYVDKKVTESRDNVVDLYKFIFPPNLTKPTFSVIGCVQPLGSITPVAELQSRVATRVLKEDVQLPTRKQMWEDIYQKRQELAKHYTHSLRHTVMVEFIDYLDELADIIGCKPRLCKLWLTDPRLAYACTFHPCAAYQYRLTGPLPWKGAREAIFTQWERTLFPLKHRTMKETNETHTRFQYFIMLCTMVTVVTAILTYILFVGHI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The desired protein should be able to perform H(+) + hexan-3-one + NADPH + O2 = ethyl butanoate + H2O +   NADP(+)2. The presence of FAD is necessary for the protein to function in the desired environment.3. A protein that can perform NAD(P)H oxidase H2O2-forming activity, hypotaurine dehydrogenase activity, NADP binding in endoplasmic reticulum membrane to support lipid metabolic process. The designed protein sequence is ",
    "response#1": "MTERR",
    "response#2": "MNL",
    "response#3": "VGIVL"
  },
  {
    "reference": "MKPRNVTSIRSEHSQAMVEEGDEPVYHSQGNSSGSAAELPPSATEKQSYASVENGRVDIDLNSGLCRRLSRLVPASPVPQTRPPESPPEYSEQGPQSLRLNIVVQIVGSRGDVQPFVALGQELQRHGHRVRLATHDVFAAFVRDAGLEFFPVGGDPAELMAYMVKNPGLLPSMQGLRGGEVQRKRRMVAGMLRGFWSSCVEPDPVTSVPFVADAIIANPPSFAHVHCAQALGVPLHLMFTMPWTSTTAFCHPLANLVGSDAIPPRTANFLSYLAVEWLTWQGLQDVINSWRRTLDLEPVPFSEGPFLAETLQIPVTYCWSPALVPKPADWPSYIDVCGFFFRKEPEYAPTPELAGFLKSGQRPVYIGFGSIVVDDPRALSATIAAAVKEAGVRAVVSRGWSKLGEGQVASTDVFYLDDCPHEWLFKQVSAVVHHGGAGTTACGLLNGRPTVVVPFFGDQPFWGTMVAAAGAGPDPIPAQQLDVAKLAAAIRFCLTPEAAAAAESMSARMRAESGVRRAVASFHANLPLEKMRCDLLPSQPAAWMCTSPKRLKLSKAAAEVLTGAELISWSSLRRYNPKPIHISNRRWDPVTATASSLAATSTGMVTSAADIVIKPMEALGKSLTRAEHGGADAIGAPGGHDAVYGRSAAIELPRATNVQPTGRGTAAAAALGSAVGLGNFFKSFTKGMLLDLPLAVTEGMRNAPRLYGGDVYEPGDVVDWKSGGLAAGRNLSHGFVEGFRGLVAEPMRGAEQGGAVGAARGAGVGILN",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein design should emphasize Polar residues compositional bias.2. The AMP binding site should be stable and able to withstand changes in environmental conditions.3. For general function, the protein need meet that Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.4. The protein contains novel Adenylate kinase active site lid, Glycosyltransferase family 28 N-terminal domains that confer a unique function or activity.5. A protein that contributes to ITP metabolic process, phosphorylation, ADP biosynthetic process, AMP metabolic process by functioning in mitochondrial matrix with nucleoside triphosphate adenylate kinase activity, adenylate kinase activity.6. The protein should be designed to catalyze the following reaction a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'-   diphosphate + ADP. The designed protein sequence is ",
    "response#1": "MMLDFTKTDARNALKGRFGYDEFRPGQEEAITCVLEGSDTLAVLPTGFGKSLCYQLPPLALEGLTLVVSPLISLMKDQVDGLKARGIAAEALNSTQSRAEVDDILNGRARLLYMAPERFRNAAFLERLSKVAISLFVVDEAHCISEWGHDFRPEYLRIAKVLDLLGRPQTVALTATATPAVQRDIQEKLRLREPRVFVSGFDRPNIRFSVRRVEGREDKLLALLKEVSGPWLLDGFPRTVTYVRSKRDTEALAEFLRSEGIGAAAYHAGLKNAERRRNQDAFMTDQIRVMVATNAFGMGIDKPDIRFVIHAEVPESIEAYYQEAGRAGRDGLPAECVLLYHPADHFRHRFMVEQSYPPIAQVRKVYDALVDYCATGACRRRVLLEYFGEAFAAGEACGKCDNCLHEMEEWDGTEAAKQLLSIVHRTEQRFGAGHVISVLRGSRSEKVKAHGHDKLSTFGILKDHTEAQIRYWVRQLAAEGFLSVSADHYPVLRLSARSAPVLRGEQEIPLPPMEQRAAAARESAHRSGDLRWIHPPSGRVYNLEFNPPHIHGIDDITGEPLVQQDSA",
    "response#2": "MSNRYAELGLTATQGREAKVRWIHPPSGRVYNLDFNPPHVHGIDDITGEPLVQQEGPRMLWLAEETGRVHVWSEVAPATSGDRPAALRRVHPASGRVYHTEHNPPKVAGKDDVTGEELIQREGPRMVWLTEETGRVHVWSEVAPATSGDRPAALTRWVHLPSGRVYNTDFNAPKVPGKDDVTGEDLVQREGPRLMWLAEETGRVHVWSEVAPATSGDRPAALTRWVHLPSGRVYNTDFNAPKVPGKDDVTGEDLVQREGPRMMWLAEETGRVHVWSEVAPATSGDRPAALTRWVHLPSGRVYNTDFNAPKVPGKDDVTGEDLVQREGPRLMWLAEETGRVHVWSEVAPATSGDRPAALTRWVHLPSGRVYNTDFNAPKVPGKDDVTGEDLVQREWPRMVWLAEETGRVHVWSEVAPATSGDRPAALTRWIHPPSGRVYNLEFNPPHIHGIDDITGEPLVQQDGPRLLWLAEETGRVHVWSEVAPATSGDRPAALT",
    "response#3": "MKILGVNIDHIATVREARHTTYPDPVQAAFIAEQAGADGITIHLREDRRHIQDRDLKILRETVTSRLNLEMAPTEEMLNIVMRIKPDACTLVPEKREELTTEGGLDVAGHLEKIVKAVEELHQAGIEVSLFIDPDKEQIKAAAATGADAIEIHTGAYAESRDREEELAELEKIRQGAEYAKSLGLIVNAGHGLHYHNVKPIAAMEEIYELNIGHAIIARAAFDGLDKAVRDMKRLMLEAQC"
  },
  {
    "reference": "MFRYLFVLSRPRFWLYLAGPVLVGVSYGATTVGELFSAPAVVLFSYFLLPANIYLYGINDVFDRDVDETNPKKDGRESRYRGGAAVAVIVAVCGVFLGFVAAPLPAEAWPYLAAWFVLATEYSAPPLRFKTTPVLDSLSNGLYVLPAAAAYAGVSGTHPPLLAVAGGWLWAMGMHTFSAIPDIEPDRAAGIQTTATALGADRALAYCAGIWLLSAAVFALVDVRFGLLLLAYPVLVFGIRRLQVAVGRAYWWYPAVNTLVGMVFTLGGLWGVVHG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The protein should be optimized for stability and solubility when expressed in plasma membrane where it will be involved in carotenoid biosynthetic process. The designed protein sequence is ",
    "response#1": "GGVSTAARLARAGFHVTVLEKNNFTGGRCSLIHHEGYRFDQGPSLLLLPGLFHRTFAELGTSLEQEGVKLLKCEPNYMIHFSDGEKFTLSSDLSVMKTEVEKWEGKEGYTRYLEFLKESHGHYELSVREVLLRNFEGLTAMLRPEFLRHLLQLHPFESIWTRAGKYFWTERLRRVFTFGSMYMGMSPFDAPGTYSLLQYTELAEGIWYPVGGFHRVVEALVKIGEREGVDFRMETAVKKILLSEDGGVAKGVELEDGRRLEADVVVNNSDLVYAYEKLLPIKTPYAESLKGRPGSCSSISFYWALDRQVPELEAHNIFLADEYRESFDSIFKKHLIPDEPSFYVNVPSRVDSTAAPEGKDSVVVLVYG",
    "response#2": "GGVSTAARLARAGFHVTVLEKNNFTGGRCSLIHHEGYRFDQGPSLLLLPGLFHRTFAELGTSLEQEGVKLLKCEPNYMIHFSDGEKFTLSSDLSVMKTEVEKWEGKEGYTRYLEFLKESHGHYELSVREVLLRNFEGLTAMLRPEFLRHLLQLHPFESIWTRAGKYFWTERLRRVFTFGSMYMGMSPFDAPGTYSLLQYTELAEGIWYPVGGFHRVVEALVKIGEREGVDFRMETAVKKILLSEDGGVAKGVELEDGRRLEADVVVNNSDLVYAYEKLLPIKTPYAESLKGRPGSCSSISFYWALDRQVPELEAHNIFLADEYRESFDSIFKKHLIPDEPSFYVNVPSRVDSTAAPEGKDSVVVLVWSAR",
    "response#3": "GGVSTAARLARAGFHVTVLEKNNFTGGRCSLIHHEGYRFDQGPSLLLLPGLFHRTFAELGTSLEQEGVKLLKCEPNYMIHFSDGEKFTLSSDLSVMKTEVEKWEGKEGYTRYLEFLKESHGHYELSVREVLLRNFEGLTAMLRPEFLRHLLQLHPFESIWTRAGKYFWTERLRRVFTFGSMYMGMSPFDAPGTYSLLQYTELAEGIWYPVGGFHRVVEALVKIGEREGVDFRMETAVKKILLSEDGGVAKGVELEDGRRLEADVVVNNSDLVYAYEKLLPIKTPYAESLKGRPGSCSSISFYWALDRQVPELEAHNIFLADEYRESFDSIFKKHLIPDEPSFYVNVPSRVDSTAAPEGKDSVVVLVRF"
  },
  {
    "reference": "MADIKAIAEQLVNLTVKEVSELATILKEEYGIEPAAAAVAVAAAPGAAGAAAEEEKTSFDVVLKSAGAAKLQVVKAVKEQCGLGLKEAKDLVDAAPSTVKEGVDKATAEALKKALEEAGAEVELK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that Is thus essential for accurate translation. The designed protein sequence is ",
    "response#1": "TTVRNLGILAHVDAGKTTVTERILYLTGTTHKRGEVHDGTTVTDFDPQERDRGITIFAAAVSCAWAGHRINLIDTPGHVDFADEVERSLRVLDGAVAVFDAVAGVEPQSESVWRQADRHGVPRIAFVNKMDRAGADLDAAVASIRERLHPVPLVVQLPIGTEDGFTGVVDLPRMRALVWADGADAAEEGPVPGTLREEAARRRRVLEEAVAERHPGALEEFCDRETLTAATLTGALRDLTRTGDGVVVLCGSAYRNRGVEPLLDAVVAYLPSPVDVPAVK",
    "response#2": "RNLGILAHVDAGKTTVTERILYLTGTTHKRGEVHDGTTVTDFDPQERDRGITIFAAAVSCAWAGHRINLIDTPGHVDFADEVERSLRVLDGAVAVFDAVAGVEPQSESVWRQADRHGVPRIAFVNKMDRAGADLDAAVASIRERLHPVPLVVQLPIGTEDGFTGVVDD",
    "response#3": "GSRLDNVVYRMGFGSTRAEARQLVSHRTVMVKKAGRDEFIRVNIPSIQLQDGDVIAIQEKSREQLRIKNAIS"
  },
  {
    "reference": "MRYLVTGSQMRAIDQDTIQNIGIPSLVLMERAAECVARKAEQLCDQKTLQILAICGVGNNGADGVAAARMLYLHGFDVLVVVVGNEAHATKEFEVQKGIAEKLEIPVVDWEHFLHGEDEQKAGDRAIIDAVFGVGLSRDVEGTYAEVLDWINKTETEWVLAVDMPSGIHSDTGAVMGTAVKADVTVTFGYEKMGSALYPGRGYCGQTEVCDIGFPELSRKKAGAEQFTYDLEDLNRIPARPAYSNKGTFGKVLVVAGSADMSGAAYLCAKAAYSMGAGLVKIMTPQENRQILQTLLPEAILSSYRNGQEPENLEKLSSQVEKNCGWADVIVLGPGMGQGSTAKFLVQEFLSNAYVPMIIDADGLNIIASDPDLTGFYTENLILTPHLGEMARLTGKTVPEIQEDLPSCGAQYSSDSGVICVLKDAATVVTGRDGQCYVNSSGNSAMAKAGSGDVLSGTIAGLLALGMENWDAAVLGVYIHGTAGDRVLEKKGSHGLLARDLTEEIAAITTEIGKDEPVREHCAIEDRICFRRK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should be able to bind AMP ligand in a variety of conditions2. The designed protein must be able to perform NADPHX epimerase activity under specific conditions. The designed protein sequence is ",
    "response#1": "THKGDFGHAVLFCGEKEGAGVIAAMAAARYGSGLTTLVIHEPIMHPPYLMTASVVPPNASAIGIGMGLGSYFEDEFLTHYVIKSHLPIVLDADALHVPKLLSILEQKDRQIVITPHPKEFVSLWKILTGEQLSVSQIQAKRFEIVRRFNTKYPHVTLLLKGANTLIVQEEKLYINPLGCSKLSKGGSGDVLSGLIVSLLAQGYTALDAAIQGSLALVLAAGKYTGASYAMLPTDLIDR",
    "response#2": "LLIAGSKGMAGASILSAKACLRSGIGLLTIHAPICNLQILQTTVPEAITQIDAHESCFACPVETQKYNAIGIGPGLGESPESELAFLDLLEQVNIPLVLDADALNFIGRNKNSIKNIPYDTILTPHPKELDRIIGNCTNSYSRLSQATDLAQATRSYIVLKGAYTTIITPDGNCYFNPTGNPGMATGGSGDILTGIILALLAQGYSSFDASLIGTYVHGLAGDLAKENKGEIGMTAQDIVDYLK",
    "response#3": "LMIAGSYGKIGAAVLSTEACLRTGAGLVSCHIPRCGYEIMQARLPEAMVSTDLNHSINTHIETDLDVYPAIGIGPGLGTASETRTLLKEILSVYKRPVVLDADALNSIAKEPELLAQIPAGSILSPHPKEFLRLFGETANEFERLQLASLKAKELKLIIVLKGHHTLIALPDGKMFFNSTGNAGMATGGSGDVLTGILTGLLAQGYEPGDAAVFGVYLHGSAGDLAAEELSMEAMIAGDITKYY"
  },
  {
    "reference": "MAVHAPYNHAPPPTQEINGQKPTLAPAITLERPADCINRGQYPAFYIICGYLNRLRADAPHKKYELLTRIFGNWREKVGPDLYPLIRLLLPDKDRERPVYNLKESMLARCYIDILSLEKHSEAAQRLIKWKQPAGNSPNPTGDFAKVCYNEIKARSTVEEGQLSVEAVNMLLDKLAVGKMKQKDYVPILKAINMQCTAEEQEWIIRIILKDLHISIRERGVLSAFHPDAIDLYNVCSDLKRVCWTLYDPGFRLNKNETHLELFHSFLPQLCGRMNDASLENIAKAIGAPKEFIMEEKLDGERIQLHMRGNGAQWFYCSRKAKDYTYLYGAHPGEGSLTRYIATAFQDNVRNVILDGEMMVWDPVVERYLAFGTLKSAALAFSLVKVFDILFLNDHCLSRKRLSERKRLLRSGKIFKNIENYKGRLEFVDEKRGKNAKDIREYLERVVETKGEGLVVKKTDVIYQTNSRGYDWIKVKPEYSDQMGENLEVLVLGGWWGKGGRSGKISRLLCGLREQAFDDGTLQDFLFNRFWNELLGLRVDFVSHLMHLRSMRCSPTHLSQCRNKHKKHWRPFDRSNPPPFMKLGPVGLDDKPDVYIEPENSFVIEVKASEIVPAGYGIGFTLRFPRCKYIYYDKNSRDYALDDECLWTVGQGFFYKTRKLIKRTDFMDLFSRPKRSYDDSQGPGNFRGQKLSDADVETSIFSDMTFSDLEALVHKHGADFTQAQLSDLSAIVISPDQKNPLVRAQIRHGVNVIKPEWVFESIARRTAL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The ATP binding site should be located in a region of the protein that is accessible to the ligand.2. A protein with DNA ligase (ATP) activity, ATP binding, metal ion binding is needed to enhance nucleotide-excision repair, DNA gap filling, DNA ligation involved in DNA repair in a specific context.3. The designed protein must have a N6-AMP-lysine intermediate that is structurally stable and resistant to denaturation.4. The protein must exhibit the following characteristics: Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologous end-joining.. The designed protein sequence is ",
    "response#1": "MLAQVAESLESAVREMRTAAVEWKFDGSRVQVHWDGSRVTIYSRRLENVTNALPDIVEEIKKSVKPGVILDGEVIAVKEGKPMPFQHVLRRFRRKHDVAKMVEKIPLEAHFFDILYHDGECIDLPLRERRKLLESAVNESEKIKLAKQIVTDSVDEVRKMYDEAISAGHEGVMIKLPSSPYIPGKRGKNWLKVK",
    "response#2": "PMLSKSVNQIPPGMSYEPKWDGFRSILFRDGAEVELGSRKERPMTRYFPELVAAALTELPDRCVIDGEIVLPADNHLDFEALQLRLHPAASRVAMLAEQTPAAFIAFDLLALGDDDYTGRPFSERRAALETALADAGPTFHLTPATTDLPTAQRWFHEFEGAGLDGVIAKPLDLTYQPDKRVMFKVG",
    "response#3": "PMLSKSVNQIPPGMSYEPKWDGFRSILFRDGAEVELGSRKERPMTRYFPELVAAALTELPDRCVIDGEIVLPADNHLDFEALQLRLHPAASRVAMLAEQTPAAFIAFDLLALGDDDYTGRPFSERRAALETALADAGPTFHLTPATTDLPTAQRWFHEFEGAGLDGVIAKPLDLTYQPDKRVMFKVFK"
  },
  {
    "reference": "MKKDTGFDSKIEKLARTTASKTGRRGFIGRLGGFLVGSALLPLLPVDRRSRLGGEVQAATTGNLTRSGFKPQDKDPKACEYWRHCTIDGNLCDCCGGTLTSCPPGSSLSPSSWVASCYNPGDQQTYLIAYRDCCGKQTCGRCNCVNTQGELPVYRPEFNNDIVWCFGADNDAMTYHCTISPIVGKAS",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. tryptophan tryptophylquinone residue is required for the proper folding and stability of the protein.2. The designed protein should interact with enzymes and substrates within the One-carbon metabolism pathway to enhance or regulate its activity.3. The protein design should aim to mimic or enhance the function of natural proteins involved in amine metabolic process, potentially with improved properties such as stability, specificity or selectivity. The designed protein sequence is ",
    "response#1": "VFGLSRAIRKAEKNAIIAELKVYSPKYGDLLKGRNPFEILRAYERAGAVGISYITDPKYFRGSFEFLRKLCRETELPVLRKDFIASKEEVERTAEAGASAVLLITRLLKEELPEFVDFAKEHGLDTLVEVHSEEELAIALQTDSTMIGINNRDIGKLELDDGNVSLTEKLAPLIPKRYVKVSESGIAGTEDLKRALRHADAALIGTALMKTPDPEEFLG",
    "response#2": "LSRAIRKAEKNAIIAELKVYSPKYGDLLKGRNPFEILRAYERAGAVGISYITDPKYFRGSFEFLRKLCRETELPVLRKDFIASKEEVERTAEAGASAVLLITRLLKEELPEFVDFAKEHGLDTLVEVHSEEELAIALQTDSTMIGINNRDIGKLELDDGNVSLTEKLAPLIPKRYVKVSESGIAGTEDLKRALRHADAALIGTALMKTPDPEEFLRKR",
    "response#3": "LSRAIRKAEKNAIIAELKVYSPKYGDLLKGRNPFEILRAYERAGAVGISYITDPKYFRGSFEFLRKLCRETELPVLRKDFIASKEEVERTAEAGASAVLLITRLLKEELPEFVDFAKEHGLDTLVEVHSEEELAIALQTDSTMIGINNRDIGKLELDDGNVSLTEKLAPLIPKRYVKVSESGIAGTEDLKRALRHADAALIGTALMKTPDPEEFLRKR"
  },
  {
    "reference": "MIDLLGLDLDGTLLSKTKKINNPSKLALTNLIAKKPSLKVMILTGRSVFSTLKHVEKLNSLFKKPIVDYFCCYGGAKLYQIEANKPQERYKFCLENSVVETTFSIIKKHRGLCLAYLDSYVSPYLCLAGNKLLGWFTKYFWYRKRCVFFNQNHLKQGILKISVYFLSAKRCKKVYEILKNTFQEKVNVLSFSNNLIEITHHDANKGYAIEYMAKREQLSLNRIAVIGDSWNDYAMFKKAKYSFAMSKSPSQLKLIATNTSNKTNRYRFSTLLNLISETIINQKAD",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The Nucleophile active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The designed protein should have a binding site that is specific to the target phosphate ligand.3. The protein must be able to phosphatase activity. The designed protein sequence is ",
    "response#1": "MDLLRFITCGSVDDGKSTLIGRLLWDSRQIFEDQLAVLKTDSKRHGTQGEQVDLALLVDGLAAEREQGITIDVAYRFFSTARRKFIVADTPGHEQYTRNMITGVSTADLAVILIDARLGILMQTRRHAYLVSLMGIRHIVLAINKMDLVDFDAKVSQSIEAEFNHLTQTLNFKSVTPIPISALTGDNITSRSSHMPWYLGPTLMELLETIDVTRPL",
    "response#2": "MDLLRFITCGSVDDGKSTLIGRLLWDSRQIFEDQLAVLKTDSKRHGTQGEQVDLALLVDGLAAEREQGITIDVAYRFFSTARRKFIVADTPGHEQYTRNMITGVSTADLAVILIDARLGILMQTRRHAYLVSLMGIRHIVLAINKMDLVDFDAKVSQSIEAEFNHLTQTLNFKSVTPIPISALTGDNITSRSSHMPWYLGPTLMELLETIDVTRPMDLLRFITCGSVDDGKSTLIGRLLWDSRQIFEDQLAVLKTDSKRHGTQGEQVDLALLVDGLAAEREQGITIDVAYRFFSTARRKFIVADTPGHEQYTRNMITGVSTADLAVILIDARLGILMQTRRHAYLVSLMGIRHIVLAINKMDLVDFDAKVSQSIEAEFNHLTQTLNFKSVTPIPISALTGDNITSRSSHMPWYLGPTLMELLETIDVTRPR",
    "response#3": "MDLLRFITCGSVDDGKSTLIGRLLWDSRQIFEDQLAVLKTDSKRHGTQGEQVDLALLVDGLAAEREQGITIDVAYRFFSTARRKFIVADTPGHEQYTRNMITGVSTADLAVILIDARLGILMQTRRHAYLVSLMGIRHIVLAINKMDLVDFDAKVSQSIEAEFNHLTQTLNFKSVTPIPISALTGDNITSRSSHMPWYLGPTLMELLETIDVTRPMDLLRFITCGSVDDGKSTLIGRLLWDSRQIFEDQLAVLKTDSKRHGTQGEQVDLALLVDGLAAEREQGITIDVAYRFFSTARRKFIVADTPGHEQYTRNMITGVSTADLAVILIDARLGILMQTRRHAYLVSLMGIRHIVLAINKMDLVDFDAKVSQSIEAEFNHLTQTLNFKSVTPIPISALTGDNITSRSSHMPWYLGPTLMELLETIDVTRPLRVADRFVFPVQWVNRPSSDFRGFSGTIASGHIAVGDPVRITTSGQITHIKRLVTFDGDLSAAHSGDAVTLVLDKEVDASRGDIIAAKNAPAATPGQTVEYRLRRANSVRQFEATLIWMQEEAGLTGSNYEIKLANQWARAAITSIKYGININTLAHESYRQLGINDIAVCDIALHRPLVLDAYEQSPTLGGFILVDRFTQATVAAGLIDHALRRATNVHRQALSISDRERAALNGHKGATIWLTGLSGSGKSTLANALEVKLHALGKHTYILDGDNVRHGLNKDLGFTEADRVENIRRVGEVAKLMMDAGLIVITAFISPFRAERDMVRRMIPAGEFIEIFVDTP"
  },
  {
    "reference": "MGLAATRTKQRFGLDPRNTAWSNDTSRFGHQFLEKFGWKPGMGLGLSPMNSNTSHIKVSIKDDNVGLGAKLKRKDKKDEFDNGECAGLDVFQRILGRLNGKESKISEELDTQRKQKIIDGKWGIHFVKGEVLASTWDPKTHKLRNYSNAKKRKREGDDSEDEDDDDKEDKDSDKKKHKKHKKHKKDKKKDKKDKKEHKKHKKEEKRLKKEKRAEKTKETKKTSKLKSSESASNIPDAVNTRLSVRSKWIKQKRAALMDSKALNEIFMITND",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Involved in rRNA-processing at A0, A1 and A2 sites through its action in U18 and U24 snoRNA 3'-end final trimming The designed protein sequence is ",
    "response#1": "GLTVILPWDYAKFVWRKMMYQKGIRFGGLENLHQIAFEKRMPFFPIDYPDTISGQLCEEERKKRNEDSWKRRPPAKRVNYQKFGDNFSEIGNPFCCDV",
    "response#2": "GLTVILPWDYAKFVWRKMMYQKGIRFGGLENLHQIAFEKRMPFFPIDYPDTISGQLCEEERKKRNEDSWKRRPPAKRVNYQKFGDNFSEIGNPFCCDS",
    "response#3": "GLTVILPWDYAKFVWRKMMYQKGIRFGGLENLHQIAFEKRMPFFPIDYPDTISGQLCEEERKKRNEDSWKRRPPAKRVNYQKFGDNFSEIGNPFCCDL"
  },
  {
    "reference": "MERVNKLCVAFFVINMMMAVAAAQSATNVRATYHLYNPQNINWDLRTASVYCATWDADKPLEWRRRYGWTAFCGPAGPTGQASCGRCLRVTNTGTGTQETVRIVDQCRNGGLDLDVNVFNRLDTNGLGYQRGNLNVNYEFVNC",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Ensure the presence of a signal peptide within the protein.2. The designed protein must have at least one Barwin domain.3. A protein that can perform ribonuclease activity in extracellular region to support defense response to bacterium, defense response to fungus. The designed protein sequence is ",
    "response#1": "DMTSSQWFKTQHVQPSPQACNSAMSIINKYTERCKDLNTFLHEPFSSVAITCQTPNIACKNSCKNCHQSHGPMSLTMGELTSGKYPNCRYKEKHLNTPYIVACDPPQQGDPGYPLVPVHLDKVC",
    "response#2": "DMTSSQWFKTQHVQPSPQACNSAMSIINKYTERCKDLNTFLHEPFSSVAITCQTPNIACKNSCKNCHQSHGPMSLTMGELTSGKYPNCRYKEKHLNTPYIVACDPPQQGDPGYPLVPVHLDKVC",
    "response#3": "SSQWFKTQHVQPSPQACNSAMSIINKYTERCKDLNTFLHEPFSSVAITCQTPNIACKNSCKNCHQSHGPMSLTMGELTSGKYPNCRYKEKHLNTPYIVACDPPQQGDPGYPLVPVHLL"
  },
  {
    "reference": "MEKFILNVEKRERTGKGIARQLRSKGIIPCVIYKNGQSTPVQIPTKELYTFMNIATREKLFVTLKLDGQEKQAILQDYQVDPVTGKLLHVDFMEVSATEKIRVTIPVVLIGEPIGVKQDKGVLQHGISEIEIEAIPEKIPGHIEVDVSHLEVGDAVHVSDIKFEEGIKVISNPEEVIATVTVEEEEAEVAPSIEETVEPEVIKKGKKAEEEEEK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance.2. The protein should be optimized for cytosolic large ribosomal subunit-specific folding and stability, while maintaining 5S rRNA binding. The designed protein sequence is ",
    "response#1": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTS",
    "response#2": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTF",
    "response#3": "PIGCKVTLRGQRAQQFLETALGIVDKTLVRSQFDSLGNVSFGIEEHTDFPGMKYDPNIGVFGMDVTVVIKRPGERICKRRIATRKIPTF"
  },
  {
    "reference": "MVIAKKIIVVNPFVGVPKHNDFDIVQEELSELQESEFLAEAIYFSVDPYQRMKLGNVYPCDMIGGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKPENEKLCQQKPYLYKLPDFEHLPVSLGLGVCGRVGNTAYFGFSQICQPKPGETVVVSGAAGAVGSHVGQIAKIMGCVVIGFAGTDEKCQWLIQEAGFDHVANYKTADIPTFLKGTAPNGVDCYFDNVGGELSSVVLSHMRTFGRVAVCGAISTYNETNADKLKATVVQPSIVSKQLKVEGFQVNRFADRNMEGIQKNLAWVKSGQLKYKEHLTEGFESVIDAFIGLFRGDNIGKSIVKVT",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have 15-oxoprostaglandin 13-oxidase activity, 2-alkenal reductase [NAD(P)+] activity to facilitate prostaglandin metabolic process. The designed protein sequence is ",
    "response#1": "GLPKVSDFKLVEEDTPELKNGDLLCETLFISVDPYMRLFGRSLKEGDTMIGEVVSRVIESKSKKYPVGTTVLAGYGWRTHAVVHEDDKHPLPTAQLRAVPADFPKELSPGLLLGVTGMPGMTAYFGLIDVCQAKSGETVFVNAAAGAVGSLVGQIAKIKGCKVIGYAGTDDKVTWLRDLGFDYVFNYKTKSLGEELKKAAPEGIDCYFDNVGGDFSVSVLTSHMKDHGRVAVCGSISTYNNPEAKGQYVFETIIIKRLKLQGFIGAEYQADWPAAATQVAKWIL",
    "response#2": "GLPKVSDFKLVEEDTPELKNGDLLCETLFISVDPYMRLFGRSLKEGDTMIGEVVSRVIESKSKKYPVGTTVLAGYGWRTHAVVHEDDKHPLPTAQLRAVPADFPKELSPGLLLGVTGMPGMTAYFGLIDVCQAKSGETVFVNAAAGAVGSLVGQIAKIKGCKVIGYAGTDDKVTWLRDLGFDYVFNYKTKSLGEELKKAAPEGIDCYFDNVGGDFSVSVLTSHMKDHGRVAVCGSISTYNNPEAKGQYVFETIIIKRLKLQGFIGAEYQADWPAAATQVAKWIL",
    "response#3": "GVPKKTDFELVEQDLPPVKDREVLFEALFISVDPYMRAISPKLPTGITMIGTQVAKVLESKHPDYKKGDLVLGPFGWVTHTVQNPDEVLSPFGEKTKPYVLPDLGGFSPSTALGVLGMPGNTAWFGLTELCKPKSGEVLVVSGAAGAVGSLVGQIGKILGLTVIGFAGSDEKCKMLVKELGFDYAFNYKTKPVPEALQEAAPNGVDCYFDNVGGRMSNDVMNHMNMFGRVANCGSISVYNSDPKDPPLAPVLQPTIVMKQLTLQGFISSRWKDRWFEGIQKNLQFMKEGKLKFKETMVEGFENMFEAFTMLFTGENIGKTVL"
  },
  {
    "reference": "MKLLAMTVLLLTICSLEGALVRRQAEEQSLQSLVSQYLQTVTNYSKDLMEKAKAPELQAQAKAYFEKTQEQLTPLVKKAGTDLFNFLSNFMDLKTQPAAP",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. A protein with cholesterol transfer activity, high-density lipoprotein particle receptor binding functions is required to enable cholesterol homeostasis, negative regulation of lipase activity, cholesterol metabolic process.2. Design the protein with a signal peptide incorporated. The designed protein sequence is ",
    "response#1": "RMTKPAALLLNQETARLIQASFKHAKFPNITGERSMRFLGTVAYTLANIQVSDLSIEQSEVELKENDAIDIAIKNVTAFFRGTLTYGYAGAWFLQLFHSVDFEIQSSIDLQINIKLLCQEEQVAADASDCYLSFHKLMLHLQGDKEPGWLKQLFTDFISFTLKFVLKRELCKEINLLAQVMANFVHNVAENFVQDEAIGLDISLASDPLIKANYLESHHEGLVLYKNP",
    "response#2": "LESHHKGHFTHKNVSEAFPLRAFPPGLLGDSRMLYFWFSDQVLNSLARAAFQEGRLVLSLTGDEFKKVLETQGFDTNQEIFQELSRGLPTGQAQVAVHCLKVPKISCQNRGVVVSSSVAVTFRFPRPDGRH",
    "response#3": "LESHHKGHFTHKNVSEAFPLRAFPPGLLGDSRMLYFWFSDQVLNSLARAAFQEGRLVLSLTGDEFKKVLETQGFDTNQEIFQELSRGLPTGQAQVAVHCLKVPKISCQNRGVVVSSSVAVTFRFPRPDGRI"
  },
  {
    "reference": "MNLITDIIIAVAGVGVGVAAGYVIRKNIAEAAIGSAEAQAKKIIDEATKAAEAKKREAVLEAKEEILKMKNEVEREHRERRQELQRLERRLLSKEETLDRKIESFERKEEQLAKKEQEIENLRQSLEETLQKELAELERISGLSTEEARELLLKQVEEEVQQEMALLIKEIETKAKEEAEKRAREIITLAIQRCAADHAAETTVTVVALPNDEMKGRIIGREGRNIRTFESLTGVDLIIDDTPEAVILSGFDPIRREIARRALEKLIQDGRIHPARIEEMVERATKEVEQDIREAGEQAAFEVNVHGLHPEIIKLLGRLKFRTSYGQNILKHSIEVAHLAGLMAAELGADVALAKRAGLLHDIGKAVDHEVEGSHVEIGVELAKKYREHPEVLHAIATHHGDEEPKTVEAVLVQAADAISAARPGARRETLEAYLKRLEKLEGIANEFEGVEKSYAIQAGRELRILVKPEKIDDLASVRLAREIAKKIEEEVEYPGQIKVTVIRETRAVDYAK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein should have a specific function within the plasma membrane.2. The protein should have Helical domains to ensure proper insertion into the membrane.3. The protein contains novel HD domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "GTLSILAAILMETKLHSPMYFFLGNLSLPDVGCVSVTVPAMLSHFISNDRSIPYKACLSELFFFHLLAGADCFLLTIMAYDRYLAICQSLTYSSRMSWGIQQALVGMSCVFSFTNALTQTVALSPLNFCGPNVINHFYCDLPQPFQLSCSSVHLNGQLLFVAAAFMGVAPLVLITVSYAHVAAAVLRIRSAEGRKKAFSTCSSHLTVVGIFYGTGVFSYTRLGSVESSDKDKGIGILNTVISPMLNPLIY",
    "response#2": "GTLSILAAILMETKLHSPMYFFLGNLSLPDVGCVSVTVPAMLSHFISNDRSIPYKACLSELFFFHLLAGADCFLLTIMAYDRYLAICQSLTYSSRMSWGIQQALVGMSCVFSFTNALTQTVALSPLNFCGPNVINHFYCDLPQPFQLSCSSVHLNGQLLFVAAAFMGVAPLVLITVSYAHVAAAVLRIRSAEGRKKAFSTCSSHLTVVGIFYGTGVFSYTRLGSVESSDKDKGIGILNTVISPMLNPLIS",
    "response#3": "GTLSILAAILMETKLHSPMYFFLGNLSLPDVGCVSVTVPAMLSHFISNDRSIPYKACLSELFFFHLLAGADCFLLTIMAYDRYLAICQSLTYSSRMSWGIQQALVGMSCVFSFTNALTQTVALSPLNFCGPNVINHFYCDLPQPFQLSCSSVHLNGQLLFVAAAFMGVAPLVLITVSYAHVAAAVLRIRSAEGRKKAFSTCSSHLTVVGIFYGTGVFSYTRLGSVESSDKDKGIGILNTVISPMLNPLIS"
  },
  {
    "reference": "MATLPVSLVFAFKNRPKCVITRAQYVKEATDLAKRSNELGSPTRRKDESFGKISIQELVFLEDDVQALNEAFPQGEKADTSWAVTVLFSLAKLVFGILGLALSVIWPLHIIVFMLVNPPAFPFLNQVFIQLDSAWGLLGTTAFAIFCYYLVMSVISGEMHSIHPMK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should have a Helical domain that is long enough to span the entire membrane.2. The designed protein should be expressed specifically in the plasma membrane3. The designed protein should contain Cytoplasmic, Extracellular topological domains. The designed protein sequence is ",
    "response#1": "GPEQAAVLNCSLGAAAAGPPTRVTWSKDGDTLLEHDHLHLLPNGSLWLSQPLAPNGSDESVPEAVGVIEGNYSCLAHGPLT",
    "response#2": "GRTANGKVRLYVIGQGALSAAVAANSKHDIAEAERWNLPIILIVLLAVFGSLAAAAVPLALGVCTVVVTMGLVDLVSMHTIMSVFVTSTVSMFGIALAVDYSLFILMRFREELRSGRQPQEAVDAAMATSGLAVVLSGMTVIASLTGIYLINTAALKSMATGAILAVAIAMLASITLTPAALATFGRAAVKRSVLMHWSQRSECTQSLFWTRWVGWVMHRR",
    "response#3": "GNLGMILLIAVSPLLHTPMYYFLSSLSFVDFCYSSVITPKMLVNFLGKKNTILYSECMVQLFFFVVFVVAEGYLLTAMAYDRYVAICSPLLYNAIMSSWVCSLLVLAAFFLGFLSALTHTSAMMKLSFCKSHIINHYFCDVLPLLNLSCSNTHLNELLLFIIAGFNTLVPTLAVAVSYAFILYSILHIRSSEGRSKAFGTCSSHLMAVVIFFGSITFMYFKPPSSNSLDQEKVSSVFYTTVIPMLNPLISALNY"
  },
  {
    "reference": "MQFIDQAKIEVEAGKGGDGIVAFRREKYVPTGGPSGGNGGRGGSVFFVADENLQTLLDFRYNHRFQAEKGTRGGPNNCTGAGGKDLIIEVPCGTTIYDAETGELLGDLTEPQQTLLIAQGGKGGLGNQHFLSNRNRAPEYALPGLPGEIKQLRLELKLLAEVGIIGLPNAGKSTLISSLSAARPKIADYPFTTLIPNLGVVRKPTGDGTVFADIPGLIAGASHGAGLGHDFLRHIERTRVLLHLIDATSDDVIRDYNTIKEELQAYGQGLAERPQILALNKIDAVDRETVDLEALATQLNHLSYAPVFIISAVTRTGLEPMLQEIWGILDQMKVPEEVEALR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a specific function within the cytoplasm. The designed protein sequence is ",
    "response#1": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECN",
    "response#2": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECQ",
    "response#3": "LWGSELNQEKQTCTFRGQGEKKDSCKLLLSTICLGEKAKEEVNRVEVLSQEGRKPPITIATLKASVLPMVTVSGIELSPPVTFRLRTGSGPVFLSGLECS"
  },
  {
    "reference": "MAPSARTTKHIFVTGGVASSLGKGLTASSLGNLLTARGLRVVMQKLDPYLNVDPGTMNPFQHGEVFVTEDGAETDLDVGHYERFLDRELSQKANVTTGQIYSAVIAKERRGEYLGDTVQVIPHITNEIKDRILAMAEPGPDGRKPDVVITEVGGTVGDIESLPFLEAIRQVRHDIGRDNVFYLHVSLVPYLAPSGELKTKPTQHSVAELRNIGIQPDAIVCRSDREIPQKLKEKLALYCDVDREAVVSAPDAPSIYEIPKVLHREGLDAYVVRRLNLSFRDVDWKRWDDLLDRVRHPRQTVTVALVGKYIDLPDAYLSVTEAVRAAGFAHRARVDIRWVPSDDCTDPAGAAKELAGVDGVLVPGGFGVRGIEGKLGAIRYARENRIPTLGLCLGLQCMAIETLRHTAGIADANSLEFDENTPNPVISTMADQEDIVAGKGDLGGTMRLGAYDAHLLPGSIVAEAYGETVISERHRHRYEVNNAYRDAMADAGLVVSGTSPDGRLVEFVELDRDLHPFFVGTQAHPELKSRPTKAHPLFAAFIGAAIEYNDADRLPVALPEAAEETAAEETAAEAAAAEAASAQVADGEVAKKRTKKAAKAATSRNGASRASVRS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The design of the protein must focus on the precise arrangement of amino acids in the Nucleophile; for glutamine hydrolysis, Nucleophile, active site to enable effective catalysis of H2O + L-glutamine = L-glutamate + NH4(+).2. A protein with metal ion binding, ATP binding, CTP synthase activity functions is required to enable glutamine metabolic process.3. The L-glutamine, CTP, UTP, Mg(2+), ATP binding site should be stable and able to withstand changes in environmental conditions.4. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.; Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen5. The protein should be optimized for activity in Pyrimidine metabolism, CTP from UDP: step 2/2 pathway and exhibit minimal cross-reactivity with other metabolic pathways. The designed protein sequence is ",
    "response#1": "YIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRKERRGDYLGATIQVIPHITNAIKERIVEGGDNHDVVLVEIGGTVGDIESLPFLEAIRQMAVEVGREHTLYMHLTLVPHMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPNNERAKIALFCNVPEKAVISLKDVDFIYKIPALLQSQGLDDYIRINLPQLSADQKTIEDAYKSIAEALTHGGMANRVKVRIEWVDAELFDKEDATPYLQGFHAILVPGGFGERGTEGKIKAAQYAREHKVPYLGICLGMQMAVIEAARNVAGIEEAGSEEFDHEAGKKRFEPVVYHLKEWVQGNHKVSRSADDDKGGTMRLGAYDAALTEGSKVAEVYGSNAIEERHRHRYEVDIKYREQLEACGLKFTGMSPDGRLPEIVEWTDHPWFIGVQFHPELKSKPFDPHPLFKDFVDAYLSIHESLKHAGLDAATEVETTWIHSEELVDGVGDAFEGIDGIVVPGGFGSRGTEGKIAAIRYARENDVPFLGLCLGFQMAVVEFARNVLDLDGAHSAEMVENTAHPVIDLLPEQEDVDDMGATMRLGAHETQIEAGTLAHALYDSTTCTERHRHRYEVNPEYIPDLEAAGLTFSGESGRRMEILEIDDHPYFLGTQFHPEFRSRPGRASPPFVGFLKSKLGGAEQLIN",
    "response#2": "MTKYIFVTGGVTSSLGKGIISASLAKLLQSRGYRVTIQKFDPYINIDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNVPTSKANNITTGRIYQNVISKEREGAYLGKTVQVVPHITDEIKRNMRLLGDSGEYDIVITELGGTVGDIESLPFIEAVRQFRWEAGSSNSLVIHLTLIPYLAAAGELKTKPTQHSVKMLLEYGIQPDILVCRAEHHINLELRKKIALFCNVNINAVVESIDAPSIYDVPLLMLKEQLDKTVLTKLKVTIGLVGKYVELQDAYKSILEAFVHAGAVNECKVEVKNIHSEYLDKENVKSSLSGLDGILVAPGFGNRGIEGKIEAIKYARENKIPFFGICLGMQCCVIEYARNVLGYTGANSTEMDIHTAYPVIDIMEDQKNIIQMGGTMRLGAYDCEIDPNSKAYEVYGKQYISERHRHRWEFNNSFIEQFEEAGMKAVGKNPETGLVEIVELINHPFFIGSQFHPELKSTVENPHPLFIGFVKAAKENRESR",
    "response#3": "RFIFITGGVVSSLGKGLASAALGALLQARGYSVRLRKLDPYLNVDPGTMSPFEHGEVFVTDDGAETDLDLGHYERFTGVPARKTDSISSGRIYTNVLEKERRGDYLGKTIQVIPHVTNEIKDFISIGEDEVDFMLCEIGGTVGDIEGLPFFEAIRQFSQDKPRGQCIFMHLTLLPYIKASGELKTKPTQHSVKELRSIGLAPDILVCRSEGPIPVKEREKLALFCNVRADSVIAAQDLKSIYEAPLAYHREGLDQAVLDAFEIGYPELDLVEWTEKDAYKSVYESLDHAGIAHRTKVRVLRIESDKLADEGAAQALANVDGLLIPGGFDKRGIEGKIEAIRFARETGLPFFGICLGLQCATIEFARHVAGLENANSTEFDKASPHPVVCLLEEQEGVTNLGGTQRLGAYPCHLKPNTKARAAFGVDVISERHRHRYEVNNNFRDRLEAHGMIFSGTSPDGSLVEAIELKDHPWFVAVQSHPEFQSKPTKPHPLFRDFLAAAAGR"
  },
  {
    "reference": "MAAISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQKTVKKVDEAYNYVKEIASNGGTILFVGTKKQAQDSVRDEAIRSGMYFVNQRWLGGTLTNFQTIRKRINRLKSIEKMEEDGTFEVLPKKEVVNLLKEKERLVKFLGGIKEMNKLPDALFVIDPRKERIAIAEAHKLNIPIIGIVDTNCDPDEIDYVIPANDDAIRAVKLLTSKMADAILEVKQGEETEVVEEEANEAVAEEEAAEEVSTQE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein that contributes to translation by functioning in small ribosomal subunit with structural constituent of ribosome. The designed protein sequence is ",
    "response#1": "MI",
    "response#2": "KIRRMMSKGLERAGISKVDIERTRDRVRVDIHTARPGIVIGRKGAEADRIRGKLEKLTGKQVQLNIIEVKSPK",
    "response#3": "ERRLDAVVYRAKFVSTMFAARQFINHGHIKVNGKRVNIPSYKVRVGDVIEVKEASKQLAFVLEASV"
  },
  {
    "reference": "MMAMVNIEPHLLISHLRDLTSADAKVTADSRQIQSGDIFFAYPVGHGNALRDGRDYIAAALANGAAAVVFDPADGVANEYLDRPECFAIESLSNLAGELCAEWYDYPSKQLNVIGVTGTNGKTSITQWLAQALDEPNHRAAVLGTLGTGFPGALIQTGYTTPDAPRLQTQLKELLDEGAQQVAIEISSHALDQDRVAGLDVCTAVFTNLTQDHLDYHGTMGEYAEAKAKLFQLPQLKNAIINFDDAFGRELAMKLLAVGGPAVWGYALSGNAFTGFEKFGDRLKRTHAQNTVLTTAGYEAQFNLDGLGSSAVQLAVLGEFNLSNCLAVWTALLSQGLSPSEASKRMSKLSPVPGRMELIRLNKMQRTEGPLIVVDYAHTPDALTKALTALRPIANQRGGKVWCVFGCGGDRDISKRPQMGRAAQEFADHIVITSDNPRSEDPGSIIAMIEAGMSGDLSNIQALPDRAAAIMAAVRHADIKDIVLVAGKGHESTQEISGKKFDFSDQEHIRLAAGGSL",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should have ATP binding to facilitate peptidoglycan biosynthetic process.2. The protein contains novel Mur ligase C-terminal domains that confer a unique function or activity.3. The presence of Mg(2+) is necessary for the protein to function in the desired environment.4. The protein should have a high affinity for ATP, meso-2,6-diaminoheptanedioate. The designed protein sequence is ",
    "response#1": "VTGTNGKTSIVQLLARAFSVLGRRSATIGTLGAGLHGALDAGERTTPDAISQQRLLARFVEQGASDVAMEVSSHALDQGRVNGIQFRIAVFTNLTRDHLDYHGDMSAYAAAKRKLFHLPGVGSAVINIDDAFGRSLSADLPATVACVRYAIDAADAEVRALDIDMDASGLRFLLRTPWGEQRIESKLLGRFNVANLLAVAAH",
    "response#2": "ITGTNGKTTTSYIIESILASAGFKVGVIGTINYRFSGKTFSNPVTTPESLDLQRILFEMVKEDTTHVVMEVSSHALDLFRTEACFFDAAIFTNLTHDHLDYHGDIETYWLCKKRLFTGILVSGPKKEKAFAVINCDDARGKELSETLSVKCVKTGSSAAYNVHTINSGLSTEGIEAAISTPMGSFQFRSKLVGKHNVENILCAAGLCIESGIKKEH",
    "response#3": "VTGTNGKTSIVQLLARAFSVLGRRSATIGTLGAGLHGALDAGERTTPDAISQQRLLARFVEQGASDVAMEVSSHALDQGRVNGIQFRIAVFTNLTRDHLDYHGDMSAYAAAKRKLFHLPGVGSAVINIDDAFGRSLSADLPATVACVRYAIDAADAEVRALDIDMDASGLRFLLRTPWGEQRIESKLLGRFNVANLLAVAAG"
  },
  {
    "reference": "MPPLLLAEEIRKVERTSQENLMERAGRSAATLVLERIPSGRILVLAGPGNNGGDAFVVARHLKSMGCRIDLVFASDPSRLPADAKSAHLAWIESGGTCLASIPQGKWDAVIDGLFGIGLKRDVEEPYAGFIAAVNAQSLPVFALDVPSGICSDTGRIRGIAVRASHTITFIAFKPGLLTSDGIDCCGTLHLEDLGLTSVAEEFAQGFLISPEVVAQSFPLRPRNSHKGCFGSVAVLGGEMPGAAILAARAALKLGAGKVYLCSAGSDCSQPEIMVRDAFAIASGDNAILVVGPGLGKSIQARDLVEVALRSASPLVIDADALNLIAGEPVLQELLCSRHAPSILTPHPLEAARLCACSTQEIQKDRVASARLISERFGCPVMLKGAGSVCAVPGGAFAINASGNPGLAAAGMGDVLSGMIGAYLAQGLDAERALLCAVHLHGEAADSLAEKGIGPVGLVASEIIEEARFLLNARLANPRRFRVPDGA",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. We are looking for a protein with broad substrate specificity for (6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate. The designed protein sequence is ",
    "response#1": "LPPLDAAGHKFSRGLVHALAGKMPGAIALAATAAARAGAGYVRVSTSRPIDGLPTAVVQTDKAEVNDARIGCLLVGPGMGDIPQLLTLALTSHAPKVIDADGINHVGEPDRLRGQDAVITPHADEFARLFGSTPGSKAEQALEAARRSGAVVVYKGPDTLVAAPDGRVGFAPPAPAWLASAGTGDVLAGIIATMRARGLPGFEAACAGVWLHGRAAEIAGAHMIADDLADAMV",
    "response#2": "LPPLDAAGHKFSRGLVHALAGKMPGAIALAATAAARAGAGYVRVSTSRPIDGLPTAVVQTDKAEVNDARIGCLLVGPGMGDIPQLLTLALTSHAPKVIDADGINHVGEPDRLRGQDAVITPHADEFARLFGSTPGSKAEQALEAARRSGAVVVYKGPDTLVAAPDGRVGFAPPAPAWLASAGTGDVLAGIIATMRARGLPGFEAACAGVWLHGRAAEIAGAHMIADDLADAMQ",
    "response#3": "LPPLDAAGHKFSRGLVHALAGKMPGAIALAATAAARAGAGYVRVSTSRPIDGLPTAVVQTDKAEVNDARIGCLLVGPGMGDIPQLLTLALTSHAPKVIDADGINHVGEPDRLRGQDAVITPHADEFARLFGSTPGSKAEQALEAARRSGAVVVYKGPDTLVAAPDGRVGFAPPAPAWLASAGTGDVLAGIIATMRARGLPGFEAACAGVWLHGRAAEIAGAHMIADDLADAMA"
  },
  {
    "reference": "MIVFIVLPILAAVLQQSSGNVDFDSESPRKPEIQNEIVDLHNSLRRSVNPTASNMLKMEWYPEAAANAERWAYRCIEDHSSPDSRVLEGIKCGENIYMSPIPMKWTDIIHIWHDEYKNFKYGIGADPPNAVSGHFTQIVWYKSYRAGCAAAYCPSSEYSYFYVCQYCPAGNMRGKTATPYTSGPPCGDCPSACDNGLCTNPCTQEDVFTNCNSLVQQSNCQHNYIKTNCPASCFCHNEIK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that This toxin also shows a little inhibition on cyclic nucleotide-gated CNGA1 channel.; Blocks contraction of smooth muscle elicited by high potassium-induced depolarization, but does not block caffeine- stimulated contraction; Since high potassium-treatment activates voltage-gated channels and caffeine exposure activates ryanodine receptors, this toxin may target L-type voltage-gated calcium channels (Cav) (and not ryanodine receptors) on smooth muscle2. Design the protein with a signal peptide incorporated.3. The protein should be optimized for extracellular region-specific folding and stability, while maintaining toxin activity, calcium channel regulator activity.4. The designed protein have SCP, ShKT domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "DWECLPLHSSCDNDCVCCKNHHCHCPYSNVSKLEKWLPEWAKIPDALKRCSCQRNDKDGKINTCDKYC",
    "response#2": "VDTHNSYRRSVSPTATNMLKMEWYPEAAANAERWAYQCVYDHSQKYERVLDGIQCGENIYMYSEFRPWTEVMQSWYDEYRNFIFGVGAHPANALIGHYTQIVWYKSYRVGCAIANCPSYPYNYFYVCQC",
    "response#3": "DWECLPLHSSCDNDCVCCKNHHCHCPYSNVSKLEKWLPEWAKIPDALKRCSCQRNDKDGKINTCDKYC"
  },
  {
    "reference": "MKEILTADQMRRSDQRMIQKMQVPSLVLMERAALQCVAAMKAEDIDLSKALVVCGSGNNGGDGFAIARMLVEEGNHPDVVLVGNYDHRSEETKIQMKILENLGISVGNSLPQKEYSVIIDAVFGIGLSREIKGRYAEVIDQMNRMTACKVAVDTPSGIRSDDGKVLGTAFKADLTVTFAFQKMGQILYPGCEYTGKLVTAPIGITRPEFFAEEEICMALEKSDVPAMLPQRKPDSNKGTYGKVLMITGSKGMSGAAYLSARAAYLSGAGLVRIYTEESNRAILQELLPEAVMTTYSLDETESFEELPDLLEWADVLCIGCGLGMGPHSEELLKKVLENNKTPAVIDADGLNLLAKTDEWGIEQIRMNPSGYVLTPHMKEMSRLTGYSVQELKDNRRELLRKYTEKVHAVCVLKDSRTLVKAPEGRLMINTTGNAAMAKAGSGDVLAGMITGLMAQHMRPDDAAVLGVYLHGLCGDHARKELGSYSVLAGDLLKMLGRTLKELEDMTE",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should be optimized for metal ion binding, NADHX epimerase activity, ATP binding activity in order to improve nicotinamide nucleotide metabolic process.2. The designed protein should have a binding site that is specific to the target AMP, K(+) ligand.3. The designed protein have YjeF N-terminal domains with specific sequence motifs that are crucial for its activity. The designed protein sequence is ",
    "response#1": "RSASLHTYDLRKEDQAHKFSHGHALILSGPSGKTGAARLAARGALRIGAGLVTLGVPPSAQIEVACQITALMLTQVQGAEGLATVLDDPRINALCLGPGLGRDRARDLVPVALRDPKRAVVLDADALSAFADQPEALFAMLHDKCVLTPHGGEFARLFPDLAQQLEIPAEKGPAYSKVDATRAAAARAGCMVLFKGPDTVIAAPDGRCSVHAAVRDRAAPWLATAGSGDVLAGFITGLLARGFAPLHAAETAAWLHVECARTFGPGLIAEDLPEQLPEVFRAR",
    "response#2": "GGTIMTGATRLVARAARRSGVGLLTIVSDASVLPVYAADAPGTMTASISGLEALLNDCRHNAFVVGPGYGVGDSTRGLVLQLLHLCRSTVLDADALTSFSDDPSTLCFAVQGPTVLTPHEGEFTRLFPDIDGDKLARARAAAKRSGATVLIKGADTVIAAPDGRAAISAVDAPWLATAGSGDVLSGMIAGLMAQGMEMFDAACMGVWLHGRSGTDC",
    "response#3": "GGTIMTGATRLVARAARRSGVGLLTIVSDASVLPVYAADAPGTMTASISGLEALLNDCRHNAFVVGPGYGVGDSTRGLVLQLLHLCRSTVLDADALTSFSDDPSTLCFAVQGPTVLTPHEGEFTRLFPDIDGDKLARARAAAKRSGATVLIKGADTVIAAPDGRAAISAVDAPWLATAGSGDVLSGMIAGLMAQGMEMFDAACMGVWLHGRSGTDS"
  },
  {
    "reference": "MTPFRRPIVLATSASAPFPPAEAALTDPDGLLAVGGDLSPQRLLNAYAHGIFPWYSDGRPILWWSPDPRMVFRTDGVRLSSRFKRQLRASTWTVRADTAFEQVIDACAASPRPGQDGTWITAEMQQAYIALHRLGHAHSIEVFDGARLVGGIYGVAVGRMFFGESMFSGESGGSKVALAALAADLHGRGWPLIDAQVENPHLLSMGAERLPRAEFLHDVQRQVALAEQPGSWSQRYGEHAASDLCETHLT",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The protein design must be able to perform leucyltransferase activity in order to contribute to protein catabolic process. The designed protein sequence is ",
    "response#1": "PVLIFLHGLTGYSWHSALDKTGLIELANQNNFIVLFGQGNGEITRPVRDKYGGVSFGDIYWQIENPELDMDYIRNVMNLQGTIEYCRQDDLNLLELRKMFSDKIYLIGYSNGAMYSFNMCFYDLHFSGICSMMGGYGGLAGYSNSKISEIVDRPINISLDIPIIILSGSLDEYLPASKKAFDILLGKNFSNVKFLSIPDRKHTYSRDCS",
    "response#2": "RHLVHEVTSPQAFEGLRLAGRKPWRTDTVLAVPDHNVPTTHRDQGISDPVSRIQVETLDANVHEFGITFFPMNDVRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLLQKKMKNMLVSVDGRVGPGVTAKDIVLAIIGEIGTAGGTGYAIEFGGEAIRDLSMEGRMTVCNMAIEAGARAGMVAVDETTIEYVKGRTYAPTGEQWESAVAAWRELNSDADARFDRVVRLDATAIAPQVSWGTSPEMVVPVTGRVPDPAEATDPVKASGMKRALEYMGLAANTPIEQIAIDKVFIGSCTNSRIEDLRAAAAVVRGRKVAGNVKLAMVVPGSGLVKQQAEQEGLDQVFLEAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAAVTGHG",
    "response#3": "PVLIFLHGLTGYSWHSALDKTGLIELANQNNFIVLFGQGNGEITRPVRDKYGGVSFGDIYWQIENPELDMDYIRNVMNLQGTIEYCRQDDLNLLELRKMFSDKIYLIGYSNGAMYSFNMCFYDLHFSGICSMMGGYGGLAGYSNSKISEIVDRPINISLDIPIIILSGSLDEYLPASKKAFDILLGKNFSNVKFLSIPDRKHTYSRDE"
  },
  {
    "reference": "MGKRPLVRRRGRGGMQFRAATTGKIARAKYPAFELGEQREGTVIDLVHERGRDAPLAKIRFEDGIVSYVPAVLGTRVGSSMNFGLKSEIRDGNVISVQNIPDGTTVCNVEKHYGDGGAIVKSAGGNATVFSHGEGGVVLKLPSGRFSTLNPKNRAMVGTLAGGGVSERPFMSAGGKWRRFRSKGRKYPIVRGVAQAAYVHPHGGGRHQHVGQSSTVSRNAPPGAKVGSIAARKTGRAKIKDRR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein must exhibit the following characteristics: It has been suggested to have peptidyltransferase activity; this is somewhat controversial; Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation; One of the primary rRNA binding proteins; Makes several contacts with the 16S rRNA in the 70S ribosome..2. The designed protein should be optimized for structural constituent of ribosome activity in order to improve translation. The designed protein sequence is ",
    "response#1": "MAPGACMPLGKVPLGTTVHNVELKPGAGGQLVRSAGSSARVIARDGNYTLVTLPSGEKRLILSTCHATIGAVSNADRKNTKLGKAGRSRWLGRRPSVRGVAMNPVDHPMGGGEGKTSGGRHPVSPWGFG",
    "response#2": "MAPGACMPLGKVPLGTTVHNVELKPGAGGQLVRSAGSSARVIARDGNYTLVTLPSGEKRLILSTCHATIGAVSNADRKNTKLGKAGRSRWLGRRPSVRGVAMNPVDHPMGGGEGKTSGGRHPVSPWGFRG",
    "response#3": "MAPGACMPLGKVPLGTTVHNVELKPGAGGQLVRSAGSSARVIARDGNYTLVTLPSGEKRLILSTCHATIGAVSNADRKNTKLGKAGRSRWLGRRPSVRGVAMNPVDHPMGGGEGKTSGGRHPVSPWGFR"
  },
  {
    "reference": "MADRNLRDLLAPWVANAPERALREMTLDSRVAAAGDLFVAVVGHKTDGRHYIPQAIAQGVAAIVAQADAASPNGTCCELHGVPVVYLDDLNQRLSALAGRFYQHPSDTLQLIGVTGTNGKTTTAQLLAQWTQALGKTSAVMGTVGNGLLGHVTPAENTTGSAVDVQQVLSQFVAQGAQVAAMEVSSHGLVQHRVEDVSFAAAAFTNLSRDHLDYHGDMAGYEAAKWRLFSEHRVGQAVINADDEVGRRWLERLPDAVAVSVRGDIGAARRQWLKATRVDYHNSGFTVHFDSSWGQGVIDSSLMGAFNVSNLLLSLATLLSLGYPLEQLVITGSTLQPVCGRMEVFVADGRPTVVVDYAHTPDALEKALEAARLHCHGKLWCVFGCGGDRDKGKRPLMGAIAEQLADRVIVTDDNPRSEDPQAIVSDILSGLLDAGRVQAIAGRAEAVTSAIMQAAADDVVLVAGKGHEDYQLVGNERLDYSDRITVARLLGVIV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a binding site that is specific to the target meso-2,6-diaminoheptanedioate, ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate ligand.2. The ideal protein would be one that plays a critical role in cell division, cell cycle, regulation of cell shape through ATP binding, magnesium ion binding, UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity within cytoplasm.3. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.4. We require a protein that can specifically bind to the metabolites produced in the peptidoglycan biosynthesis pathway.5. The protein design should include a Meso-diaminopimelate recognition motif motif to ensure proper function.6. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.7. The protein contains novel Mur ligase N-terminal catalytic, Mur ligase C-terminal, Mur ligase central domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "MKLKDILSGIEYKVLKGNMDIEIAGVAIDSRRVQPGALFLAYQGTRSHGLDHVDAAIAAGAAAVAWDDAQPPVLVTALTFDSRQVSADAVFFAITGTVADGHKYITQTIEAGASVIICEVMPQDINENVTYIQVENSSVALGKMAANFFGITGTNGKTTTSYLIREILKRYDKKTGVIGTIKYLIDNEEKVAVHTTPEAPEFQELLHEMLVKGVEYVITEVSSHALALKRVDYTKFDIAVFTNLSRDHLDFHKDMEDYFNAKKRLFTELLKNEGVAVINFDDVYGRRLAEQLNTKKITYSIENPQADIYVKNYRLNFKETEINLHIKKEKELVVKTHLLGIPNIYNVISAVSAALSMGIDKETILKGIESAPGVDGRMQQIENTNIFVDYSHTPDALEKALLELKNIQVLGSKLNVIFGCGGNRDKKKRSLMGQIAAKLADNVIITDDNPRLEDPKLIRAEIISGIDKANYIEIENREKAIKYGINNLNQDDILLIAGKGHETYQILKDETIHFDEREIVKKILEERK",
    "response#2": "MKLHQLIHQISIEQIVGNTNIRGITHDSRKVSEGRMFVALREAGSDGHRYIPDALKNGAAALLVERLPVEVDIPFFQVSNTRVAYAVIASNYYKQPSHKLQLVGVTGTNGKTSIVSLLHELFINEGYKSGLISTIKVEYGDKIYDNTHTTPNALLINTYLSDMVGLGITHCFMEVSSHGITQHRIEGLVFKGGVFSNLSHDHLXYHGSFKKYRDTKKEFFDNLPKSAFALTNLDDKNGAFMLQNSKAKKYTYSVKHHADYKAKLLECQFSGMLLKIKSHEVWTSLVGNFNIQNLLAVFAVGTALQLGINLEEITKAIPQLTGVPGRFEQFHSADNIVGIVDYAHTPDALENVLETITHLRHGNEKVITVVGCGGNRDAEKRPKMAAIACKYSNLVILTSDNPRYEDPLDILEQMRKGVPAQDYKKTIVIPDRREAIFKAGLEANPEDIILIAGKGHENYQEINGVRHHFDDKEILKKIL",
    "response#3": "MTVTGLTVDSRAVKEGYLFAALPGAMMHGAEFIGPALRMGAAAILTDAEGAKIAAEFLAGADVALIIAEDPREALAGAAALVYGRPSEQLTMVGVTGTNGKTSVATFLFQLFRALGYRCGLLSTVQNQIEDEIIPSTHTTPDAVALNKLLSEMLEKNCSHVFMEVSSHSVAQHRITGLHFAGGIFTNITHDHLDFHKTFDNYIKAKKGFFDQLPKSAFALVNVDDRRGSVMVQNTRAKVETYSLQTLASFKGKIISDTLAGMHMEINQQEVWFRVIGRFNAYNLLAVYGAVLAAAGVDAEAAAAGVASCAGVPGRMQRVPSNEGCFIVDYAHTPDALQQVLASLKTHCDGQLWAVFGCGGDRDAGKRPLMAQAGLAGADQIILTADNPRTEDPNIILQDMQAGMSAEQYQRTHIEPARQKAIEYAVRQAAAADIVVIAGKGHEQGQEIAGVKHPFDDAEVLRAALDDRV"
  },
  {
    "reference": "MRDLANILTPLGVTCSTLFVEHLRIDSREIQPGDAFIALRGHIQNGEKYVHAAFEQGAKCVLVDRESDVLPEDSRVIKVTDLKKRIAHIAADFYGRPSEQMKLVGVTGTNGKSTTTAMIANLAQLCDTPSAVIGTLGYGPVEQLTPLANTTPSSIDLQRILATLRETAQLVAMEVSSHGLEQDRVGQSRFEAAVFTNLSRDHLDYHGSMQAYANAKLKLFKTCHPRVSVLNIDDEYALQWLDEHTFDHLVVYGLKPAQHNFSQYVWFDDVHCSQYGIRAKIHTSWGDKDVELPLFGLFNLYNLAASLATLLMLGYDLKALLRAASKLKPVAGRMEATFTVNKPTCVVDYAHTPDALELALKALQQHVPGNVVCVFGCGGDRDVGKRALMAQAAEKYANKVIITSDNPRSEDPDKIIADVKAGLSQPQYAYCQSDRALAIKYAIEQSPNNSVILIAGKGHEDYQIIGQQRIAFCDRQWVKTILEGKGA",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein design must be focused on enhancing the efficiency of peptidoglycan biosynthetic process. The designed protein sequence is ",
    "response#1": "VTGTNGKTTIATLLYKLFSELGYKCGLLSTVQNHIGDRIVAATHTTPDALHLNALLRDMVDEGCTHVFMETSSHAIHQHRVAGLRYTGGIFSNITQDHLDYHKTFDEYIRVKKSFFDSLPSDAFAISNADDKRGAVMLQNTNAKKYFYSLKTLAEFKGRILENNLTGLVMTINDQEVHFRLIGEFNAYNLLAVYGAQ",
    "response#2": "VTGTNGKTTIATLLYKLFSELGYKCGLLSTVQNHIGDRIVAATHTTPDALHLNALLRDMVDEGCTHVFMETSSHAIHQHRVAGLRYTGGIFSNITQDHLDYHKTFDEYIRVKKSFFDSLPSDAFAISNADDKRGAVMLQNTNAKKYFYSLKTLAEFKGRILENNLTGLVMTINDQEVHFRLIGEFNAYNLLAVYGAI",
    "response#3": "VTGTNGKTTVTHLIEKIAKDRGEKTGLLGTMYRKIGEKVLPSTHTTPDVVTLQKTFLEMKEEHVTVAAMEVSSHALVQGRVHGCDFDVAVFTNLTQDHLDYHGTMEEYAHAKSLLFSQLGNAYKKPLKYAVINLDDDYAEIMLQATAVPVFTYSLEDKRAHFSVTNIQTSNTGTKFTLHSPEGTKRVTIQMVGLFSIYNVLAALAQ"
  },
  {
    "reference": "MAASGPALLVVPPGADCARHAMVQAWYRDEADDDPRRPHRAQPSRPVSLEQLRRLGVLHWKLDPDKYENDPELEKIRKERNYNWMDIITVSREELPDYEEKLKSFYEEHLHLDEEVRYVLEGSGYFEVRDDKDRWIRILVEKGDLITLPAGSYHRFTLDEQNYIKAMRLFTGQPTWEAHFRPAENLEIRKQYLEFLVQSA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The Ni(2+), Fe(2+) binding site should be stable and able to withstand changes in environmental conditions.2. The protein should be designed to have high stability and activity towards 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-   methylsulfanyl-2-oxobutanoate + formate + 2 H(+).3. The protein must be engineered to have a cooperative binding mechanism with Fe(2+), Ni(2+) to ensure proper regulation.4. The protein must exhibit the following characteristics: Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site; Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway; Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4- methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway; Also down- regulates cell migration mediated by MMP14.. The designed protein sequence is ",
    "response#1": "MRWTVMVSSKPSAPVATTDRTSRSRTPRVDHRMMDEPAPDRTGLTTGDVARLGHTGSAESLARLGLAFPAGTVAEGQELPAYDARYRTPDGTRLRLFVREGESVTTVTHACPQVCPFAAGGPYATALRSASFTGHSGGGGAGGGATAAGRVTVSGYLEGGFFHGYPTAHTLVVEVDAGPGAGAPVAALSGCCASGCAFCYVPHAFGLRQQLDPAQIVAAALLDPGVDPAELHVTGGEPTLYPDLVPIVEAVASRLPGTRLRLLTNGRMFAHPEFADALREAGVPALIPLYGSTPARHDRVTGVRGSFADTVRGLAGLRRAGRPIGVRLVLVPEVNDGEIGDLAGFLAGADPFVKFVQFMGMEPAGRALEDPRLAPRIRAAAARVAARAPGMVLANEHVPLCLLPGRFRDWATAAGRPFEALPVARRTPLEWDPDGTVTTADVPAAGGGCGCRRTRMPLLPEFRP",
    "response#2": "MSQAYNPYPLAHQSSKKSHEGDEPQWWPNQLGLNILRQHSNKSNPMGADFNYAEEFAKLDLDVLKDDVKKVMTTSQDWWPAEWGHYGPMVLRMAFWHDKDENGKALMQSM",
    "response#3": "MEKERIAAGRAKVDAYLAANPESKSFRAPDDLPADQWPSTSEKRAELKALSLPTTGGTDGKPLSYHEFRELCYDLDLSRAMKAEFDIPKEGGPQARKALGEFPIPEIFRKDPYFRAVRDQVKSGSKWPKSFLGWDSDTSVMWLVYGIKFDPEHPLLKDVPRDEAWMDFCDLFGKTYTSWPEMMKGVFGREEMMIHPSSLYLGCGDVARSNFYHVDENIYMLATGKATFYLASPDEAKGFKGPTPGEMLVKGDLIELKPGDMLFIPAHMPHSPVRNPDARAVILSFVPNQVPTKLLEDVTDLIASLS"
  },
  {
    "reference": "MPPKTKGRGRKAEARKKKKNSSPGVEAEAKHRLVLLEKELLQDRLALQREEARRAKASEDRLKQRLQGLEAELERTQSEGKAIYAEMSRQRQALKEELGTRSKQLEEEVRSLKEQLETCQREAKTAKEEAERALRKQDGTLAQLHAHVADMEAKYEEILHDNLDCLLAKLRVVKPHWDANVLRLHTRLKEQLRQFGLNPLDL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. Incorporate a signal peptide in the protein design.2. The designed protein's functionality will benefit from Basic residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "MSEAEKKASQDAQHKEPMADSETQLDSDSAPSSQAEKPAKTKYPLSFWLAFGALCLTGLISAMEGSIVSTSLPSIIAELKAAFQPLYGQLADLWGRRYVMILATAIFLLGSGICGGANSMDMLIWGRAVQGIGAGGINMLVDLIICDLVPMRERGNFIGLLFLFVSIGTTSGPIIGGALTDNTTWRWVFYINLPMGGAALVLLVLFLQVKWKKELSTRDRLKRIDVVGNAILVGATFSILYALTYGGTRYPWSAANIVAPFVLGFVGLGIFIAWESNKRWCPYPVMPLHHFNSRTASASFFISFMTMILAFWVVYFYPVYFQSVLGNTPTISGVHLLPFEVSFPIFAAVGGGLVSKTGRYKPIHMVATSIVTIAIGASSVLTQHTHKAAWAVLQIFIGMGLGSLISTTLQAVQAGLPESETAASTATWAYMRSLGTIWGVSVPAAIFNNRFDQLSGQLDPSIRDNFVRGQAYEHATAQFVQSFEPATREVVIGAYTDALRRVWLVSIAFGAVTVLSTLFEKELTLRTR",
    "response#2": "MSEAEKKASQDAQHKEPMADSETQLDSDSAPSSQAEKPAKTKYPLSFWLAFGALCLTGLISAMEGSIVSTSLPSIIAELKAAFQPLYGQLADLWGRRYVMILATAIFLLGSGICGGANSMDMLIWGRAVQGIGAGGINMLVDLIICDLVPMRERGNFIGLLFLFVSIGTTSGPIIGGALTDNTTWRWVFYINLPMGGAALVLLVLFLQVKWKKELSTRDRLKRIDVVGNAILVGATFSILYALTYGGTRYPWSAANIVAPFVLGFVGLGIFIAWESNKRWCPYPVMPLHHFNSRTASASFFISFMTMILAFWVVYFYPVYFQSVLGNTPTISGVHLLPFEVSFPIFAAVGGGLVSKTGRYKPIHMVATSIVTIAIGASSVLTQHTHKAAWAVLQIFIGMGLGSLISTTLQAVQAGLPESETAASTATWAYMRSLGTIWGVSVPAAIFNNRFDQLSGQLDPSIRDNFVRGQAYEHATAQFVQSFEPATREVVIGAYTDALRRVWLVSIAFGAVTVLSTLFEKELTLRTR",
    "response#3": "MSEAEKKASQDAQHKEPMADSETQLDSDSAPSSQAEKPAKTKYPLSFWLAFGALCLTGLISAMEGSIVSTSLPSIIAELKAAFQPLYGQLADLWGRRYVMILATAIFLLGSGICGGANSMDMLIWGRAVQGIGAGGINMLVDLIICDLVPMRERGNFIGLLFLFVSIGTTSGPIIGGALTDNTTWRWVFYINLPMGGAALVLLVLFLQVKWKKELSTRDRLKRIDVVGNAILVGATFSILYALTYGGTRYPWSAANIVAPFVLGFVGLGIFIAWESNKRWCPYPVMPLHHFNSRTASASFFISFMTMILAFWVVYFYPVYFQSVLGNTPTISGVHLLPFEVSFPIFAAVGGGLVSKTGRYKPIHMVATSIVTIAIGASSVLTQHTHKAAWAVLQIFIGMGLGSLISTTLQAVQAGLPESETAASTATWAYMRSLGTIWGVSVPAAIFNNRFDQLSGQLDPSIRDNFVRGQAYEHATAQFVQSFEPATREVVIGAYTDALRRVWLVSIAFGAVTVLSTLFEKELTLRTRRL"
  },
  {
    "reference": "MKPLVIKLGGVLLDTPAAMENLFTALANYQQNFDRPLVIVHGGGCIVDELMQRLHLPVQKKNGLRVTPSDHIEIITGALAGIANKTLVAQAAKFHLNPVGLCLADGNLTYATQLDPELGHVATVVPKNPALLNNLLGEAFLPIISSIAVDKQGLLMNVNADQAATAIAALIQADLVMLSDVDGVLDANKQRLPELNGAQIEQLIKDNVITDGMVVKVNAALDAAKMLNQGVDIANWKYPEKLTALFAGEIIGTRIKP",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein should have a low Km value for ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamyl 5-   phosphate.2. A protein should be able to manipulate the activity of enzymes in the L-arginine biosynthesis pathway, either by activating or inhibiting their function.3. The protein must exhibit ATP binding, acetylglutamate kinase activity.4. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent phosphorylation of N-acetyl-L- glutamate.. The designed protein sequence is ",
    "response#1": "TFVVKYGGHAMGDPEAARDFAEDIVLLKAVGINPVVVHGGGPQIGKMLKTLGVESRFVDGLRVTDAETARVAEMVLCGSINKEIVSWIAQAGGRAVGLSGKDGRMVVAEKVRRTQRDPDSNIEKAVDLGFVGEPADIDRRVIDTISKAGMIPVVAPIAIGEDGHTYNVNADTMAGAIAIALGAARLFLLTDVAGVLDKEKKLIRDLTPRQINALREDGTIQGGMIPKLETCVHAVEGGVDAAVIFASGTAAGALTSLLEGEALGTRFLP",
    "response#2": "TFVVKYGGHAMGDPEAARDFAEDIVLLKAVGINPVVVHGGGPQIGKMLKTLGVESRFVDGLRVTDAETARVAEMVLCGSINKEIVSWIAQAGGRAVGLSGKDGRMVVAEKVRRTQRDPDSNIEKAVDLGFVGEPADIDRRVIDTISKAGMIPVVAPIAIGEDGHTYNVNADTMAGAIAIALGAARLFLLTDVAGVLDKEKKLIRDLTPRQINALREDGTIQGGMIPKLETCVHAVEGGVDAAVIASILEPSATRRVR",
    "response#3": "TFVVKYGGHAMGDPEAARDFAEDIVLLKAVGINPVVVHGGGPQIGKMLKTLGVESRFVDGLRVTDAETARVAEMVLCGSINKEIVSWIAQAGGRAVGLSGKDGRMVVAEKVRRTQRDPDSNIEKAVDLGFVGEPADIDRRVIDTISKAGMIPVVAPIAIGEDGHTYNVNADTMAGAIAIALGAARLFLLTDVAGVLDKEKKLIRDLTPRQINALREDGTIQGGMIPKLETCVHAVEGGVDAAVILSAGRKPQAIHRLIQGEDIGTMIH"
  },
  {
    "reference": "MLSHWRLWPFLLALFMGVLESKRHPQRCSFPVLIDATSIELQDGLRRGCFKSVDLVKAYIARITEVNSALNAVLEINKDALEIAEQLDQERKQGRIRGPLHGLPILIKDLIGTSDGMETAAGSYALVGAKVREDATVVTKLRENGAIILGKTSLSEWANFRSLNSSNGWNARGGQTYGAYYLNQDPSGSSSGSGVATDLGLAAAALGTETSGSIILPGEKSNIVGIKPTVGLTSRYMVIPISERQDTIGPMARTVKDAAMLLQVIAGVDVKDNYTLASPFGKSPPDYAAACTLSGLKGKRIGVPRNVINTLGNPRTPTISTFERAISLIAGAGATIVEDANFTAYNAFLQSQAPSIVVAADFLSSLPRLEDIRHFTMHSSLEDYPSRDTGLWDLVLLARVNNTSPEFWSLYQQALRFGEEGGLLGALSRNKLDAVILPSYLASSIPGIIGSPIITVPFGAFPKATAVRYNSRANLVQEAPGIPLGISFLGAKWSEETLIAMAYAFEQQHRRGTKDLSSPSHATVFWFFLVHMEDLDNKCTSLRSQIAATEAQLAGLKRELQDAEEAALKSRTQGPASAVPDHRDLPRKWPLLGEEYRRYGRQMIVPQLGLQGQLKLRSAKVLIVGAGGLGCPAALYLAGAGVGTIGLIDGDTVESSNLHRQVLHKSQNVGKFKVDSAIEYLRELNPHPTYIAHRTHLLPPEAPEIFQKYDLILDCTDNPATRYLISDTAVLLGKTLVSASALRTEGQLMVLNNPPRPAGDKTGGPCYR",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The ideal protein would be one that plays a critical role in Mo-molybdopterin cofactor biosynthetic process, tRNA wobble position uridine thiolation, protein modification process through metal ion binding, thiosulfate sulfurtransferase activity, ubiquitin-like modifier activating enzyme activity, molybdopterin-synthase sulfurtransferase activity within cytosol.2. The designed protein should have a high specificity for Zn(2+), ATP binding, with minimal off-target effects.3. The presence of Zn(2+) is crucial for the protein to function optimally under specific conditions.4. A protein that can modulate the overall flux of metabolites through the tRNA modification pathway, enhancing or suppressing its activity as needed.5. The designed protein must have at least one Rhodanese domain. The designed protein sequence is ",
    "response#1": "DSFFQIAGLHGEPFRGAGYSSPRWWGGYCNHGNILFPTWHRAYLHRLETALRNQVSGVTLPYWNELDAETTSGPGIPDIFLTETYQFADGETIRNPLRSYTYQQTIVDRLQTMPDTDYTKPKGMQTRRFPYSSLYGAADKVTTQSHNNIMDALGIEKTNEYLNDNIKAWLADPAPGPANPNSRVGMEAGVASQYFRCLRVPNYVVFSNTTSAARWNDDNMDTPDFSPVVSLETPHNAIHVAFGGVQIPGPNTRSVPGARGDMGENETASFDPVFYFHHAFVDMVFWQWNGEAHVLVDVRPSHHFNIVSLPKSLNIPLSSLEARVSEISSALKEEVEQKGGNHSGTSVYVVCRRGNDSQRAVEYLHKIGFTSAKDIIGGLESWAMMWIQTFR",
    "response#2": "DSFFQIAGLHGEPFRGAGYSSPRWWGGYCNHGNILFPTWHRAYLHRLETALRNQVSGVTLPYWNELDAETTSGPGIPDIFLTETYQFADGETIRNPLRSYTYQQTIVDRLQTMPDTDYTKPKGMQTRRFPYSSLYGAADKVTTQSHNNIMDALGIEKTNEYLNDNIKAWLADPAPGPANPNSRVGMEAGVASQYFRCLRVPNYVVFSNTTSAARWNDDNMDTPDFSPVVSLETPHNAIHVAFGGVQIPGPNTRSVPGARGDMGENETASFDPVFYFHHAFVDMVFWQWSASDYKRISELKESCTVIDVRDQTQFGICKLENSINIPITQLMNETATNTDAVSHVRSLIFPDATRADEKAPIYFVCRLGNDSQLAVRKFKELGFDRNGERFVGDIRGGFKSWKAEVDP",
    "response#3": "DSFFQIAGLHGEPFRGAGYSSPRWWGGYCNHGNILFPTWHRAYLHRLETALRNQVSGVTLPYWNELDAETTSGPGIPDIFLTETYQFADGETIRNPLRSYTYQQTIVDRLQTMPDTDYTKPKGMQTRRFPYSSLYGAADKVTTQSHNNIMDALGIEKTNEYLNDNIKAWLADPAPGPANPNSRVGMEAGVASQYFRCLRVPNYVVFSNTTSAARWNDDNMDTPDFSPVVSLETPHNAIHVAFGGVQIPGPNTRSVPGARGDMGENETASFDPVFYFHHAFVDMVFWQWDTPRLWLLDVREKELFDMGAINGAVNIPFSRIQRCQKKTQSKELDALPDWLPEGMRTDMAPIYLVCRIGNDSQVVTRQLKELGLDKEGKRFIGDVKGGIRAWKSDIDSTIP"
  },
  {
    "reference": "MSLISTVDDHTQDTASVASGSSATKKKYNPKVYHDDNQPLSKEAMYRAKLKYGVYNSPISGGVGVGVTDYKAATNKAANVANDNQTTVEAYRRLYVDQGAASAALKVGTEPVREKPDVHAEKFKQNRALNLNYTSAAAKALSVGTEKLFTASEEARHAGQKTYSIVSQVSAASTSRAMDMSKVLKGAERKAESRLKERSEPERKEYVKSYSMTAGAAADYGAAGGAAGAAGAAGAASRSIDLNSDVMARVAKRSELPPKQEGPTEKERNAAKFALGAATAVKDLDPKSMLPEDFAAREQQRQEFVRHMTSQKVLSMAREKVDREMASIDKQQAERRLYDNDSYNRAAVAIAQQNYQNKLNAQSEKAGKINIGGGLFLTPQEVDNIAHGLISPVLGEVSERAEAQRAADTEIATRIATYEKDLSNWKNLQRTKQTNDKNVLEVNSKRIALEKQEAKDAAQKKYDEMIKKMDETVAEKKKQLEAAKQRLEDLQEEMNMKLGMQDQKVEEELQKWDENREKDIEAARLEQEELVKPFQDELDEAEKQHEEFLKERDGIDTEITGLQEAIEGHKKKIEIYEGDIRAHDNMHVEEGGKLENLGQNKETLANTLNNDIIILANKTKEQAELSTKQARLKQLEVDAMVNERKSELNQTEIQLKKEKLQLLESMRNKAQARGDEKIDEEKVKGLFGMTSEEYLAKHAPKKEEPAEEKLETVAEEASAMKKEKPTAAAATTAAAAAATTPKKPASAPRAAPAAASPKSEKKRKGSVF",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be expressed specifically in the plasma membrane, eisosome2. Target a Polar residues compositional bias in the protein's composition for improved properties.3. The protein must exhibit the following characteristics: Required for normal formation of eisosomes, large cytoplasmic protein assemblies that localize to specialized domains on plasma membrane and mark the site of endocytosis.. The designed protein sequence is ",
    "response#1": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPV",
    "response#2": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPQ",
    "response#3": "RHQRLVLQCYPDGQAADKKPNPSELSYLLFYVNHRRVKLEKVGPFLENKCYKDVSRGRQGNVMVALDIFAKLIEECHEDLNLFAQNVVNTLLDVVNSGDLLLCQHSNKVFALFCQYHDGGLFLGDPEFVRSFKQLLEVYVNMAKVPNGPNSVQWKIVGLEAAKSIAGSAAIATQTGSTSISPIVHLLLSSLQESQSDLEQLDHSLDLGGGLPDNIGGSKRNSMHQDAIDNFIHEESPEKQVRYLSFHALRTFFDTTSVVQIQSATRAIVTYIINSNVPEHWATSLVVIVAKWAPVQYRFVILVSLVEMLVAMSPNNVKSELVVAALIHALLSSTVNMVGLSVMDILGSLLNKQAAIIKHAQSNNEASGAFEELIDKLSACMISLGTHIYYADQISDMVGEVLWRCTDPQ"
  },
  {
    "reference": "MDTAQDKAGTTHFGFRDVPVAQKQKLVAEVFTSVAGKYDLMNDAMSLGIHRLWKRFFVATTQVRKGDRVLDLAGGTGDIAALLKDRVGETGELVLGDINGDMLRVGRDRMTDRGFVRGFEYVQCNAECLPFPDASFDLVTMAFGLRNVTDKDAALREIARVLRPGGQARVLEFSEVQAEWFRPIYDFHSFKVLPKLGSLFAGDAESYQYLAESIRKHPPQRELKAMMEAAGLARCSYRNMSGGIVAIHTGYKV",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for S-adenosyl-L-methionine binding.2. The protein design should consider the endogenous proteins and enzymes present in the Quinol/quinone metabolism, menaquinone biosynthesis, menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2 pathway to avoid interference with native metabolic processes.3. We are looking for a protein with broad substrate specificity for a 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol + S-   adenosyl-L-methionine = a 6-methoxy-3-methyl-2-all-trans-polyprenyl-   1,4-benzoquinol + H(+) + S-adenosyl-L-homocysteine.4. The protein must exhibit the following characteristics: Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2).. The designed protein sequence is ",
    "response#1": "MNLDELLRQIQIVGPVEAVGALGVXVXGXRXSXGXAXMPXGPXMPXGXDMXFGXNQXXAIWPKXVGIVTXRXEXVXGXSXAXAXPXRDXALXASXAFFGXPSXSLXVIGVXGATXGXPXKXVCEXXSRXEXIAXGAAXEXXEXPSXSXXNPXSXXTGXWXXIIVXGXVAXGXSXXXRXXVXEXXXGXGXXAXSXVXNWXDTXHXHVXIXQGXDXTITGXXWXQIIQ",
    "response#2": "MKVSGTVSAPQSGFTHGDLSELTGSWGAAAAGSALDPDITIVSIATDSRQAAPGGLFVAVAGHRADGRRFVPDAVSRGAVAAIVQVPADGEGVEAGGAPGALGPDVPLIEVPDVRRSLALLAAGWYGHPSRAMDTYDLMNDIMSFRLHRKLVYDVANRVLSMGIDKSPEQASRSFVALGNGIVTGELPGAPGPRIEILSPAQLTTLLDLVAAEGASGCDLDGVAFEGYGAVGDPTARPEALAGADAVFAEVGLLPDDLVPDPARYGVRGGETRAALLRRVLEGR",
    "response#3": "MSSLTGRFGEARVTSAGRDGTGAVAVPPVAVERMTGLSGAELADLFRSERVDLVFAPDESGSVAAVAVTSADPRRAFGAACRHFYANRLLAPTVALGEHPKVPVLAGVAAVSGAPVADGLQVVGWYGADHGGAGPVLAARPDPVPGPAGVGHPDAGHVDAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAGHADAG"
  },
  {
    "reference": "MLLPQVFYTPQQVKCGELRAARAQGLEMFRLMERAGQAVFTVALAQYPGSHHWLVCCGSGNNGGDGYIVARLAQSVGIQVTLWQYGDPERLQGDARTAYYHWIDSGGEVLPCTETLPDDVDVVIDGLLGTGLKGEVRSSMKQLIQTINDSGIPVVSIDLPSGLCSHTGTVLGSAICAQHTISLIGLKQGLVTGKARDHVGKLHFAGLGLEETFNNQNTATLKAIDPQAAQKALPGRKRTAHKGQQGKVLLIGGNSGMGGAAILAAQACVRTGSGLTAALVHPDNTQPVLISCPEMMTSSWMDTVMFDYRLDWCSVLAIGPGLGRDKMAQQLINRVQSITKPKVMDADALYFLAQSACYDDQRIITPHPAEAARLLGCGVENIETDRFQAIKALHQKYGGVVVLKGAGTLVCNGQDTYVCLAGNPGMASGGMGDVLTGVIVSLLGQGLALFDAAKIGVLIHSQAADQNAEAFGERGLIASDLIPHLRRLVNR",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration2. The protein design must be able to perform NADHX epimerase activity, ATP binding, NADPHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity in order to contribute to nicotinamide nucleotide metabolic process.3. The designed protein must have at least one YjeF N-terminal domain.4. The protein should have a high affinity for (6S)-NADPHX. The designed protein sequence is ",
    "response#1": "MRALDVNAEYFGISRLQLMENAGGSVAAEIALRFKPKETRVAVFCGLGGNGGDGFVAARHLACLGFNVNVILAGKSSEISVEESRRNWAALKHLRRSVTIYEVHDSSLIPNIEADVIVDALLGIGLKAPPRPPIAQLIRAINDSDAFRVAVDVPSGLDSDTGAVLGEAVKANLTVTFHKAKPGLLKAKEYVGELIVRQIGIPS",
    "response#2": "AHKGDFGRVLVVAGSRGMTGAASLVGEAALRSGSGLITVAVPETLHDILEAKLTEVMTVPLPDTGNGNLSLGARQRILSLLEGVNVLALGPGLSTEPEVATMVRELLPLVRVPCVLDADGLNALAGNVEILRKIQTPVVITPHPGEMARLMGVTTREIQEDRLSVASKAAASWNVVALLKGARTVVAAPGGAIYINPTGNPGMATAGSGDVLTGIVASLISQGLDPALAASAGAYIHGMAGDMGAREKGMIGLTAGDIITYLS",
    "response#3": "MAAVDVNAEALGVPRKQLMESSGNAVAQAIRDIAEPGARIAVVAGRGNNGGDAFVAARFLDAYDLSVHLLGRAETITTDIARENWGALERGEYDIEEVRDSTQLSLPDADVVIDAMLGTGVTGALREPAASAAEAINASDATVVSVDVPSGIDADTGEAAGVAVEADHVVTFHDDKPGLEGVDADVTVADIGIPDAAELIVAGSVNYTGAAWLAGRAAYKSGAGLVTMAVPQSLHTTLAGQFPEATWLLLPDELGVIAGDAWRVLLAGMERATAILIGPGFGLEETTRAFLANLFAAGAHTRRAGIGLVPSVKTAETQPSTKLPPVVLDADGLKLTARLPNWHKQVPAPAVLTPHPGEMSVLTGLTKETIQRERVKIAEQYARDWGHVVVLKGAFTVIAAPDGQTAVIAVATPALARAGTGDVLAGLIVGLRAQGLEAFPAAVAGAWIHAQAGLAAQEHLGA"
  },
  {
    "reference": "MSKKYDVAILGATGAVGEAMLSILEQRNFPVGKLFLLASSRSAGSTMTFCNQETTVQDVGDFDFSQVQIGLFSAGSSVSEKYAPIAAAAGCVVIDNTAQFRYEDDIPLVVPEVNPHAIAQYKNRGIIANPNCSTIQMLVALKPIHDAVGIERINVATYQAVSGTGKEAIEELAEQTRALFNHQEVKTEVYPKRIAFNVLPHIDVFMDNGYTKEEMKMVWETRKIMENESIMVNATAVRVPVFYGHSEAVHIETQKKITADEARALLEKAPGVQVMDGREPGGYPTAIEAAGQDATLVGRIREDISHPRGLDLWVVSDNVRKGAALNSVQIAEILIETYLA",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The designed protein should interact with enzymes and substrates within the L-threonine from L-aspartate: step 2/5, Amino-acid biosynthesis pathway to enhance or regulate its activity.2. The designed protein is the optimization of the Acyl-thioester intermediate to achieve high catalytic efficiency for L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-   L-aspartate + H(+) + NADPH.3. The protein design should aim to mimic or enhance the function of natural proteins involved in diaminopimelate biosynthetic process, 'de novo' L-methionine biosynthetic process, potentially with improved properties such as stability, specificity or selectivity.4. The designed protein should be able to compete with endogenous proteins or inhibitors for phosphate, substrate binding.5. For general function, the protein need meet that Catalyzes the NADPH-dependent formation of L-aspartate- semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl- 4-phosphate. The designed protein sequence is ",
    "response#1": "NIALVGATGLVGRTVTKILEERNFPINNLYLFASSKSKGKVIEFKGHENEVEELTEASLLEKELDIAFFTAGAEVSKKYVPLLREKGVVVIDNSSAFRMDEDVPLVVPEVNPEDIAWHEGIIANPNCSTIQMVVALNPLHKKYKIKRVVVSTYQSVTGTGKKAVDQLMDERAGIEGAKAYPHKIDLNVLPHIDVFLENGYTKEEMKMVNETKKIMGDDSIKVTSTTVRIPVMGGHSESVNIEFEQDFDLAEVRNIVAHTEGVILQDDPQNNIYPMPMNAHGRDEVFVGRIRRDESQANSLNLWIVADNLRKGAATNAIQIAEFLLGALHAAYGLRELVVSSYQAVSGAGRAGVETLRRQIALVAGTELGTSPGDVRRAVGEDTGPFPEPVALNVVPWAGTPREDGWSSEEMKVRDESRKILGLPKLPVAVTCVRVPVVTTHSLTVHARFEDTVTVDGAREIIATAPGVVLFDNPEAGEYPTPADVVGTDPTWVGRVRRALDDPTALELFVCGDNLRKGAALNSVQIAEYLALYKAGLVEWMSTMTYQAASGGGAQHMRELLTQFGSVYGEVKSLLDDPKSAILEIDRKVLAKQKALSADETANFGVPLGGSLIPWIDKDLGLGKSQDDAEWGMSKEEWKGGAETNKILGQGPGFDAPGIPVDGFCVRVGAMRCHSQALTFKLKKNVPLADIEAMLAQDNDWVRVVPNTREDTLKGLTPVAVTGTLQIPVGRIRKLAMGPEYVGAFTIGDQLL",
    "response#2": "MNLAVVGATGAVGTVMLDLLSTREDVWGEIRLIASPRSAGRRLTVRGEQVEVIALSEEAFDGVDVAMFDVPDEVSAAWVPVAAARGAVCVDNSGAFRMDPEVPLVVPEVNPDDLEPIRQKYGLKRVIVSTYQAVSGAGAQAVEELYEQTRAIINNEPYEPKILPVRSDKKHYQIAFNAIPQIDKFQENGYTFEEMKMINETKKIMGLPDLQIAATCVRLPVATGHSESVYIEVEKDGLTVQECKEILAGAPGVELQDDIENQIYPMPADCVGKYEVFVGRIRQDLNKANGFHMWIVSDNLLDYKNNIAVVGATGAVGQQIIKTLENRNFPVGKITLLSSARSAGKTALFKGEEVTIQEAKPESFEGVDIALFSAGGSVSKELAPEAVKRGAIVVDNTSAFRMDADVPLVVPEVNESALHEYQNRNIIANPNCSTIQMLVALKPLHDRWRITRGVVNTYQSVTGTGQAAVAQMEAERRGEKGQGVYPHSIDQNCLPHCDAFQENGYTREEMKVHQETKKIMGDSSVQLSCTAVRVPVMGGHSEAVYLELAEDFELEDVRESLNAFPGVVVEDEPSQAVYPMPLKAQGRDEVFVGRLRRDLANPRGLHLWIVADNLRKGAALNSIQIAEVLVKKYL",
    "response#3": "MSKYAVVGATGNVGREILQILSERGVAAKAVAAVASSRSTGMEVSYGEEDVLKVDALDDFDFSGVDMVLSSPGAKVSAEFAPRAAAAGAVVIDNSSHFRMDPDVPLVVPEVNPDALAGYSTRRGIIANPNCSTIQMVAALKPLHDRWRITRGVVNTYQSVTGTGQAAVAQMEAERRGEKGQGVYPHSIDQNCLPHCDAFQENGYTREEMKVHQETKKIMGDSSVQLSCTAVRVPVMGGHSEAVYLELAEDFELEDVRESLNAFPGVVVEDEPSQAVYPMPLKAQGRDEVFVGRLRRDLANPRGLHLWIVADNLRKGAALNAVQIAEILVRDYL"
  },
  {
    "reference": "MPSPLPLVFDAPKRGLPPRHLADLSLDERKEAVVSLGEKAFRAKQLSHHYFARLSVDPAQMTDIPASARERLTAALMPQLLTEVRSVACDHGMTRKTLWRAHDGTLIESVLMRYPDRATLCISSQAGCGMACPFCATGQGGLQRNLSTAEIVDQVRSAAAVMRDGLLPGPDGKPSPGRLSNIVFMGMGEPLANYRRVVAAVRRITEPAPSGLGISQRSVTVSTVGLAPAIRKLADEGLQVRLAVSLHTPDDELRDTLVPVNNRWPIDEVLRAARYYIERTSRRVSIEYALIRGVNDQPWRADLLAKRLREHLGHLVHVNVIPLNPTPGSKWDASPKEVEREFVRRVNAGGVNCTVRDTRGQEIAAACGQLAAEQN",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.2. The designed protein should have a binding site that is specific to the target S-adenosyl-L-methionine ligand.3. The protein can be expressed in cytoplasm and perform tRNA binding, rRNA binding without triggering undesirable cellular responses. The designed protein sequence is ",
    "response#1": "SQVGCAMGCDFCATTKTGFERNLTAGEMVGQFVALRREAHAAGRTLRTLVFMGMGEPLLNLDAVLVAVRRIADNALGGVGWRGITVSTVGIVPGIDALASTGLNIHLAVSLHAPDDATRGRLLPMGRRHSIADILAAADRFQARRGRPVTIQYCLLAGVNDSPEY",
    "response#2": "SQVGCAMGCDFCATTKTGFERNLTAGEMVGQFVALRREAHAAGRTLRTLVFMGMGEPLLNLDAVLVAVRRIADNALGGVGWRGITVSTVGIVPGIDALASTGLNIHLAVSLHAPDDATRGRLLPMGRRHSIADILAAADRFQARRGRPVTIQYCLLAGVNDSPERAT",
    "response#3": "PEEWEALLSSWGEPKFRAKQVFGWIHGKGVCDPEQMTNLPKRLRAKLVEDGLAPPLEVASEHLSEDGTRKLLLRMHDGRTVESVLIPQGSSGAEDPIGGEEPEDGRPVTQCISSQVGCAMACVFCASGMAGLKRNMTAGEIIAQLLLGRDRVAEGDRLRNVVLMGMGEPLHNYGAVARALTLMFHADGLGFGTRRVTLSTSGLVPEIDKLGEDFGGKIQLAISLHVPDDERRSALMPINRKYPLGALMEAVRRYPLPPRRRITIEYTLVRGYNDELEDAGRLAKLLEGLRVKVNLIPMNPVPDNPLGPPSWEVVEAFHDRLWDAGVPTFIRRRKGDDIAAACGQLALAGEKKKR"
  },
  {
    "reference": "MLNDIKNNAQTRMAKSIDALKHTLTSIRTGRATPALLDRVTVNAYGNASTPLNQVASISNADAHSLLVTPFDKGMIKEIEKGLYNAEFTPNTLGTAIRINMPPPTEERRKELVKQVQKEGEGAKIAIRNIRQDANKEIAKLVKDKAISEDEKKRGEDDIQKLTDTNIKDVDKVVADKEKELLSV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. For general function, the protein need meet that Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis The designed protein sequence is ",
    "response#1": "LKDNFNKTLNIRTAPGSLDHITVVTADGKLALNQIGQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPKVTREHREMLVKLAKQNTNKAKENLRKVRTNAMNKLKKSKDKTSEDTIRLIEKQISQMADDTVAELDRHLAAKTKELV",
    "response#2": "LKDNFNKTLNIRTAPGSLDHITVVTADGKLALNQIGQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPKVTREHREMLVKLAKQNTNKAKENLRKVRTNAMNKLKKSKDKTSEDTIRLIEKQISQMADDTVAELDRHLAAKTKELV",
    "response#3": "LKDNFNKTLNIRTAPGSLDHITVVTADGKLALNQIGQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPKVTREHREMLVKLAKQNTNKAKENLRKVRTNAMNKLKKSKDKTSEDTIRLIEKQISQMADDTVAELDRHLAAKTKELF"
  },
  {
    "reference": "MATNRRVERVASLIKREVSQMLMSGIKDDRVGAGMVSVTDVVVSGDLQHTKIFVSIYGTPEAKAETMEGLKSAASYVRSELGQRVRLRRTPEVIFVEDKGLERGTQVISLLEQLTREREAREAAQAAAGVVDSTEDA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be designed to localize specifically to cytoplasm in order to perform its role in maturation of SSU-rRNA more effectively.2. The protein must exhibit the following characteristics: May interact with the 5'-terminal helix region of 16S rRNA.; Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes); Required for efficient processing of 16S rRNA; One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. The designed protein sequence is ",
    "response#1": "MAKIRL",
    "response#2": "MSKLVLV",
    "response#3": "MDLFGD"
  },
  {
    "reference": "MLKLEMMLVVLLILPLFYFDAGGQVVQGDWRSDGLARYLQRGDRDVRECNINTPGSSWGKCCLTRMCGTMCCARSGCTCVYHWRRGHGCSCPG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. Include a signal peptide as an essential component of the protein.2. The protein should be optimized for extracellular region-specific folding and stability, while maintaining ion channel regulator activity, acetylcholine receptor inhibitor activity.3. For general function, the protein need meet that Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them; This toxin specifically blocks mammalian neuronal nAChR of the alpha- 7/CHRNA7, alpha-3-beta-2/CHRNA3-CHRNB2 and alpha-4-beta-2/CHRNA4-CHRNB2 subtypes . The designed protein sequence is ",
    "response#1": "LCCSDPRCRYRC",
    "response#2": "MYRL",
    "response#3": "PCCNPACGRHYSCG"
  },
  {
    "reference": "MNPNQKITCISATGVTLSVVSLLIGIANLGLNIGLHYKVNDSSNVNIPNMNETNSTTTIINNNTHNNFKNITNIIVNKNEEGTFRNMTKPQCEVKSGHIVSKDIAMETCEDAHILGCREPYLTRGPQGSRMFAHGTGTDLRRRQALGTWHVGHAFRALIAWKMGQAPSPYNARVECIGWSNAYCHDGISRMSICVVGPKPIASILVWYRGRPVTQNPSCVWNILLTQESECVCHKGICPVVMTDGPANNRAATKIIYFKEGKIQKIEELKGSAQHIEECSCYGAAGIIKCICRDNWKGANRPVIIIDPEMMTHTSKYLCSKILTDTSRPNDPTNGNCDAPITGGSPDPGVKGFAFLDGENSWLGRTISKDSRSGYEMLKVPNAETDIQSGPTSYQIIVNNQNWSGYSGAFIDYWANKECFNPCFYVELIRGRPKESSVLWTSNSIVALCGSKERLGSWSWHDGAEIIYFK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein is the optimization of the Nucleophile to achieve high catalytic efficiency for Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-   (2->8)- glycosidic linkages of terminal sialic acid residues in   oligosaccharides, glycoproteins, glycolipids, colominic acid and   synthetic substrates..2. The designed protein should be able to compete with endogenous proteins or inhibitors for Ca(2+), substrate binding.3. The stability of the protein-Ca(2+) complex should be optimized to maximize enzymatic activity.4. The designed protein should have at least Helical transmembrane domains to ensure proper localization.5. The designed protein should be involved in carbohydrate metabolic process, viral budding from plasma membrane by localizing to host cell plasma membrane, virion membrane and exhibiting exo-alpha-(2->8)-sialidase activity, exo-alpha-(2->6)-sialidase activity, metal ion binding. The designed protein sequence is ",
    "response#1": "LAGNSSLCPVSGWAIHSKDNSVRIGSKGDVFVIREPFISCSHLECRTFFLTQGALLNDKHSNGTIKDRSPYRTLMSCPIGEVPSPYNSRFESVAWSASACHDGISWLTIGISGPDNGAVAVLKYNDIITDTIKSWRNNILRTQESECACMNGSCFTVMTDGPSNGQASYRIFKIEKGKVVKSVELDAPNYHYEECSCYPDSGEVICVCRDNWHGSNRPWVSFNQNLEYRIGYICSGIFGDNPRPHDKTGSCGPVDSNGANGVKGFSFKYGNGIWIGRTKSTSARRGFEMIWDPNGWTGTDKNFSIKQDIVGPTDWSGYSGSFVQHPELTGLDCMRPCFWVELIRGRPKENTIWTSGSSISFCGVNSDTVNWSWPDGAELPLAGNSSLCPVSGWAIHSKDNSVRIGSKGDVFVIREPFISCSHLECRTFFLTQGALLNDKHSNGTIKDRSPYRTLMSCPIGEVPSPYNSRFESVAWSASACHDGISWLTIGISGPDNGAVAVLKYNDIITDTIKSWRNNILRTQESECACMNGSCFTVMTDGPSNGQASYRIFKIEKGKVVKSVELDAPNYHYEECSCYPDSGEVICVCRDNWHGSNRPWVSFNQNLEYRIGYICSGIFGDNPRPHDKTGSCGPVDSNGANGVKGFSFKYGNGIWIGRTKSTSARRGFEMIWDPNGWTGTDKNFSIKQDIVGPTDWSGYSGSFVQHPELTGLDCMRPCFWVELIRGRPKENTIWTSGSSISFCGVNSDTVNWSWPDGAELPQ",
    "response#2": "LAGNSSLCPVSGWAIHSKDNSVRIGSKGDVFVIREPFISCSHLECRTFFLTQGALLNDKHSNGTIKDRSPYRTLMSCPIGEVPSPYNSRFESVAWSASACHDGISWLTIGISGPDNGAVAVLKYNDIITDTIKSWRNNILRTQESECACMNGSCFTVMTDGPSNGQASYRIFKIEKGKVVKSVELDAPNYHYEECSCYPDSGEVICVCRDNWHGSNRPWVSFNQNLEYRIGYICSGIFGDNPRPHDKTGSCGPVDSNGANGVKGFSFKYGNGIWIGRTKSTSARRGFEMIWDPNGWTGTDKNFSIKQDIVGPTDWSGYSGSFVQHPELTGLDCMRPCFWVELIRGRPKENTIWTSGSSISFCGVNSDTVNWSWPDGAELPF",
    "response#3": "LAGSSPLCPVSGWAIYSKDNSVRIGSRGDVFVIREPFISCSHLECRTFFLTQGALLNDKHSNGTIKDRSPYRTLMSCPIGEVPSPYNSRFESVAWSASACHDGTNWLTIGISGPDNGAVAVLKYNSVITDTIKSWRNNILRTQESECACMNGSCFTVMTDGPSNGQASYKIFKIEKGKVVKSVELNAPNYHYEECSCYPDSGEVICVCRDNWHGSNRPWVSFNQNLEYQIGYICSGVLGDNPRPNDRTGSCGPISINGANGVKGFSFKYGNGVWVGRTKSTNSRKGFEMIWDPNGWTGTDNNFSVKQDIVGVNDWSGYSGSFVQHPELTGLNCMRPCFWVELIRGKPKENTIWTSGSSISFCGVNSETVSWSWPDGAELPF"
  },
  {
    "reference": "MSQQNRRVQQNTAAFPLLTAHDILECLAALDIPAQMEDLTKPTAQSTQSIYGSLLEVLMGASINSIEGPKQALLGMMEYKVLSFRSMKRTQINLSRQEMYSDTLQFMMFFKHCRRLALLCGIPDFAISDLARPDANRLRKVLSGIMNFAKFRDERMQTQARFQENLQKHQKKAVDLRRKTEELETQFQEITARNAAERPQSEQAQKRNELLKSELLELNSQRLKEVQEYEELKKERQTLLEQVNHNNRIVTQLELQIGSAKSRLVQSPDRIKRHISEMSFAIQSEKAKLASFQQKARELTNRLEVIGALEVDLRGLIDLEHSIQDQRAKTEEAKRSKSALEARLEGRQIESQGLAAKLEQLQRQLQNASHKLARQEETRKGMRERGARRIDELKAEYKVRARERGEWQKQRDDLLAEQKELESEMAAFVTKHENEINEFLHAYWTMRRQAEDYMNTMTVKLGLQTAI",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.2. A protein that can perform protein-containing complex binding in nucleus to support attachment of mitotic spindle microtubules to kinetochore, meiotic chromosome segregation, mitotic spindle organization, kinetochore organization, cell division. The designed protein sequence is ",
    "response#1": "IKQDQLLMRFTLIDPEDPAREFSIIVDVSKQVYSVPNCDPPIPSLPDLVRQLNFDRDLFAFIKRVRIAFS",
    "response#2": "LTFPRYSPDDIVAYLRSHVLVGAEARNLTKADLFATLKPEVLHMIFIRILQKVYGIRLEHFYMMPVNVDIVYPQIFEGFLPVCNLYIHMERFLPVCRVNDFQMSDVINPKAKRTARFLSGILNFVHFRECRREAYLELQLR",
    "response#3": "LTFPRYSPDDIVAYLRSHVLVGAEARNLTKADLFATLKPEVLHMIFIRILQKVYGIRLEHFYMMPVNVDIVYPQIFEGFLPVCNLYIHMERFLPVCRVNDFQMSDVINPKAKRTARFLSGILNFVHFRECRREAYLELQLI"
  },
  {
    "reference": "MELVDPNLEPWNHPGSKPSTPCTTCYCKKCCFHCQWCFLTKGLGISYGRKKRRQRRRPPQDSQTNQIPLSKQPISQARGNPTGPEESKKEVESKAETDPCD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein design should include a Nuclear localization signal, RNA-binding (TAR), and protein transduction motif to ensure proper function.2. The protein must exhibit the following characteristics: The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive; Tat can reactivate a latently infected cell by penetrating in it and transactivating its LTR promoter; Besides its effect on RNA Pol II processivity, Tat induces chromatin remodeling of proviral genes by recruiting the histone acetyltransferases (HATs) CREBBP, EP300 and PCAF to the chromatin; Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to promote processive transcription elongation by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts.3. The protein should exhibit Polar residues compositional bias.4. A protein that contributes to positive regulation of viral transcription by functioning in extracellular region, host cell nucleolus with metal ion binding. The designed protein sequence is ",
    "response#1": "MEPVDPRLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFITKGLGISYGRKKRRQRRRAPPDSQTNQASLSKQPASQPRGNPTGPEESKKKVERETETDPDP",
    "response#2": "MEPVDPNLEPWNHPGSQPTTPCSRCYCKKCCWHCQLCFLKKGLGISHGRKKRKHRRGPPQSHKDHQNPIPKQPIPPTRGNPTGPEESKKEVASKAETGPCD",
    "response#3": "MEPVDPNLEPWNHPGSQPTTACSKCYCKKCCFHCQVCFITKGLGISYGRKKRRQRRRAPQGDQTHQNPIPKQPTSQPRGDPTGPKEQKKKVETETETHPDH"
  },
  {
    "reference": "MNLNNDHGPDPENILPIKGNRNLQFIKPTITNENILVGEYSYYDSKRGESFEDQVLYHYEVIGDKLIIGRFCSIGPGTTFIMNGANHRMDGSTYPFHLFRMGWEKYMPSLKDLPLKGDIEIGNDVWIGRDVTIMPGVKIGDGAIIAAEAVVTKNVAPYSIVGGNPLKFIRKRFSDGVIEEWLALQWWNLDMKIINENLPFIINGDIEMLKRKRKLLDDT",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Inactivates the A compounds of virginiamycin-like antibiotics, thus providing resistance to these antibiotics..2. The protein design must be able to perform acyltransferase activity in order to contribute to response to antibiotic.3. The designed protein must have a active site active site that is highly conserved among related enzymes. The designed protein sequence is ",
    "response#1": "ETGECARRVSPCSSFKLPLAIMGFDSGILQSPKSPTWELKPEYNPSPRDRTYKQVYPALWQSDSVVWFSQQLTSRLGVDRFTEYVKKFEYGNQDVSGDSGKHNGLTQSWLMSSLTISPKEQIQFLLRFVAHKLPVSEAAYDMAYATIPQYQAAEGWAVHGKSGSGWLRDNNGKINESRPQGWFVGWAEKNGRQVVFARR",
    "response#2": "ETGECARRVSPCSSFKLPLAIMGFDSGILQSPKSPTWELKPEYNPSPRDRTYKQVYPALWQSDSVVWFSQQLTSRLGVDRFTEYVKKFEYGNQDVSGDSGKHNGLTQSWLMSSLTISPKEQIQFLLRFVAHKLPVSEAAYDMAYATIPQYQAAEGWAVHGKSGSGWLRDNNGKINESRPQGWFVGWAEKNGRQVVFARI",
    "response#3": "ETGECARRVSPCSSFKLPLAIMGFDSGILQSPKSPTWELKPEYNPSPRDRTYKQVYPALWQSDSVVWFSQQLTSRLGVDRFTEYVKKFEYGNQDVSGDSGKHNGLTQSWLMSSLTISPKEQIQFLLRFVAHKLPVSEAAYDMAYATIPQYQAAEGWAVHGKSGSGWLRDNNGKINESRPQGWFVGWAEKNGRQVVFARR"
  },
  {
    "reference": "MAQMTSGLYPMFGFFICLLFLPASWEAGANTFQELQKTGEPSIFDHLLPLTPGLTRRALSDHKNSGQHPPDLPKSTATQKPKRQCNTVRLVKPVHKPIDDAKAADYGNTTVGHEPFPASEKNLSSQGKHPMARNERSADDHGSTNSEKRSDGGHSTSAPMRKISCKPVTRTSGTPVSSTETSTKLRTTSQKPETSSHDSDLIRKSTSLPVKSTEVSRTSYRTPRSLGAERHTIPFTSDKSIQLTIEHTKEATRSQSTPTKYERETRSASERISRAHVPPVENHTPSAGETTTQVSAKSTKHTEEATTSTTEKATKAPERPTVNLNTTGLVKAMENTSTAPSPHLHKTETAHQGITGSLTSRMDLRPITSEAHHLQQNTHSLPGGLHSVQEREGSNSFPAWAIVVVILMAVIILLIFLGLIFLVSCASRARHQLTQNSEDAEPEDKGGRNSYPVYLMEQQNLNLNQISSPP",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties.2. The protein should contain a Helical membrane-spanning region.3. The designed protein should have a specific function within the plasma membrane.4. The protein must contain a signal peptide for proper functionality. The designed protein sequence is ",
    "response#1": "IPAEVVLPLAGYWVSKGDMTLAGVVLAGSLGGVAGPLTLYWIGRYGGRPFLERFGKYLFIKPEALDKSDNFFKKHGGFVAFSGRFLPGIRTLISIPCGIAKMNVWVFSLYTFIAMLPITFVYVYLGD",
    "response#2": "ILRPYEKGVVERLGKYQRTVESGLVVIIPFIEAIKKVDMREQVVDVPPQEVITKDNTVVVVDCVIFYEVVDPFNAVYNVVDFYQAITKLAQTNLRNIIGDLELDQTLTSREMINTQLREVLDEATDKWGTRVVRVEIQRIEPPGDIVEAMSKQMKAERMKRAAILEAEGC",
    "response#3": "ILRPYEKGVVERLGKYQRTVESGLVVIIPFIEAIKKVDMREQVVDVPPQEVITKDNTVVVVDCVIFYEVVDPFNAVYNVVDFYQAITKLAQTNLRNIIGDLELDQTLTSREMINTQLREVLDEATDKWGTRVVRVEIQRIEPPGDIVEAMSKQMKAERMKRAAILEAEGR"
  },
  {
    "reference": "MMTKNSIEFDPYIERKAFDETKQGVKGLVDAKITEVPRIFHHRQDILTNKKPSASVSDLEIPIIDFASVHADTASREAIVEKVKYAVENWGFFQVINHSIPLNVLEEIKDGVRRFHEEDPEVKKSFFSRDAGNKKFVYNSNFDLYSSSPSVNWRDSFSCYIAPDPPAPEEIPETCRDAMFEYSKHVLSFGGLLFELLSEALGLKSQTLESMDCVKTLLMICHYYPPCPQPDLTLGITKHSDNSFLTLLLQDNIGGLQILHQDSWVDVSPIHGALVVNIGDFLQLITNDKFVSVEHRVLANRQGPRISVASFFSSSMRPNSRVYGPMKELVSEENPPKYRDITIKEYSKIFFEKGLDGTSHLSNIRI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must be engineered to have a cooperative binding mechanism with Fe(2+) to ensure proper regulation.2. The protein must have high specificity towards metal ion binding, oxidoreductase activity.3. The designed protein should be able to compete with endogenous proteins or inhibitors for Fe cation, 2-oxoglutarate binding. The designed protein sequence is ",
    "response#1": "LQNMHYGRHIDNPFMSSGRSDLSFTISLTNKADYQGGELVIETLNSEKKLKLDAGEIIIYPSTYLHSVKKVKNGERLVCVGWK",
    "response#2": "MIHHIPNVLNKEQVAEFRKLMEEANWVGGKVTAGTLSASVKRNQQLSEQDPLTHHLSDIVIKAIWQNPLFQAAALPHKIIPPLFNRYDEHESFGFHVDNSIRLIRGTAEQIRTDLSCTLFLSEPDEYEGGDLVIEDTYGYHEVKLPAGDVVLYPSTSLHEVSSITAGTRFASFFWVK",
    "response#3": "SCSGEGDAYGLHIDNPVMGKGDARLRTDLSFTLFLSPPESYDGGELEIETVFKTESVKLPAGSMVIYPSTELHRVTPVTSGERFVFVGWIQ"
  },
  {
    "reference": "MRVRPLNVPVWTLSRVGDALPGSTVSGDATVTGISLSTRDVVAGDLFAALPGATTHGVRFAAQVLEAGAVAVLTDPEGAAALPADVPRVVVDRPRARLAAFAADFYGHPSTRFTTIGVTGTQGKTTTTYLAEAALGESDAAVIGTIGTRIAGRPAASSLTTPEAPALQALFAVMAEEQVAACAMEVSSHAIVQGRVDGFVLDAAVFLNLGRDHLDFHHDVESYFQAKAALFTPEHARRAVINLDDAHGRRLTELTTLPVTTFSTEGAAADWRAVNIRAHRLGSDVTLLAPDGRETELSVPLPGAFNVSNAVAAVVALAGEGHDLQRLADGIATARGVPGRMERVEEGQSFTAIVDYAHKPEAVEAVLHALRPVTAGRLIVVLGAGGDRDRGKRPLMGEVAARLADVLVVTDDNPRSENPASIRAEVLAGAESGAAQVLEIGDRREAIAAAVDLARTGDTVVVAGKGHERGQQVGDVVHPFDDREVLAELIGDVR",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein requires Mg(2+) to activate a critical catalytic site.2. Incorporating a Meso-diaminopimelate recognition motif motif is critical for the successful design of the protein.3. A protein should be able to manipulate the activity of enzymes in the Cell wall biogenesis, peptidoglycan biosynthesis pathway, either by activating or inhibiting their function.4. The designed protein should be involved in regulation of cell shape, cell cycle, peptidoglycan biosynthetic process, cell division by localizing to cytoplasm and exhibiting UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity.5. The designed protein should have a high specificity for meso-2,6-diaminoheptanedioate, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP binding, with minimal off-target effects.6. The protein must exhibit the following characteristics: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.. The designed protein sequence is ",
    "response#1": "MKLTELVSQIGAVQAIGEPSAHVTQVAYDSRNVEPGGIFVCIRGSRQDGHQFIRESVRRGASVILTEAATDFKQESYTLFVTGTKGKTTTVWMIWKMLVAAGYEAGLIGTIEVCYSSWQQESEYTTPESYELYEILRKMADGGCKYVVMEVSSQALKLHRVEGIVFDTAVFTNLRADHIGAGEHADFAEYMDCKRQLFRRCRCGVFNQDDAYWQEMWRESGCQAVTYGFSGQSDYCALAPQLLRGSSRLGVRYELTVHDAGQAGMTKGTSFTESGEGVTEKKPEEQKKRIVVNAPGRFSVYNSLAAIAVANSLGIEFGFIQKALAGVVVPGRMEQVGTSAAGAPVLVDYAHTPDALETVLKAVRPHAHDRVVTVFGCGGDRDRTKRPAMGKIALREADHAIVTDDNPRSEDPAAIRAEILAAGPGLVEIGDRAAAIDAAIAGLGAGDVLVIAGKGHEDYQILGTEKIHFDDREQARAAIELRLKGGK",
    "response#2": "MKLKKLLSFLPGLPVQGGEAAVLRTDSRQVQPGDGFIAWPGGVTDGRAYVASALERGAAACLVELEGVTGTNGKTTTTHLIEYFLNQANLPAALLGTLYTRWPGFSQVANHTTPFAIDLQHNLAQAVDAGCQLGIMEVSSHSLVQDRVFGCPFEVSVFTNLTQDHLDYHQDMEDYFRAKALLFTPDYLQGRAVINIDDHYGQRLTIGLDKQQLWTYSINNTSADFWVSDLNYQPHGVSGILHTPEGNSPFSSPLVGQYNLENLLAAVAAFGLALGIDPAAMAQGLAACPPVPGRMEKITGDSSRATAVIDFAHTEQALRACLTASREYTSGQLWCVFGCGGDRDQGKRQGMGRAAEELADRIIITDDNPRTEDPERIVSDIREGLANPKNSCVVHNRQAAIEYALTEAKSGDLVVIAGKGHEDYQLFADRTVHFDDREAAREALLGKE",
    "response#3": "MKLSELLPDISPQLIKGNLAKEITSNSRTVRRGSLFIALRGINSQGIDFAIDAVKSGAVAVIYDSEVLPESVIQIVTDSRQVKPGVIFIAIKGANFDSHDHAEIAMNNGAAAVIIDYYQPIRLPQIIVQDTRLALARLAASWRQQHPSEQLLGVTGTKGKTTTVWMIWKMLVAAGKTGLIGLINYRIAGERLPARHTTPDALELQKLLAEMVEEEVTHVVMEVSSHALDQFRVAGLKFDVAVFTNLTHDHLDYHKTMEAYYQAKAKLFTQLGNSKHAVINIDDVYGQRIVKDTQGQVITFGIDQQADLRAENLIYQTTRSDFTLLTSKGQFEINSPLIGQHNIYNILAALAMATHLGVDLQQIVTLLPDLTVVPGRMEKITGDSSRATAVIDFAHTEQALRACLTASREYTSGQLWCVFGCGGDRDQGKRQGMGRAAEELADRIIITDDNPRTEDPERIVSDIREGLANPKNSCVVHNRQAAIEYALTEAKSGDLVVIAGKGHETYQILGDQIIPFDDRQVALDLITGK"
  },
  {
    "reference": "MYIKTLTVSDINRYIKKTLDNDFILGNCSVKGEVSNFKFHSSGHMYFSLKDKFSKINCIMFKSSVEKLNFMPGDGMKVIVKGRISLYEKEGVYQLYCSEMKPDGMGELYLAFEKLKIELEKKGLFDISHKKKIPLYAKKIGVITSPTGAAVKDIINVTRRRNKKIELLIYPSLVQGTGASDNIIKGIETFNSMEDVELIIIARGGGSIEELWCFNDEKLAEAVYSSKKPIITGVGHEIDYTIVDFVSDMRAPTPSAAAEIGVFSLEEYVQKILNYKNKLYNSVKNTVNDKKNRLAFVKKTLEVNNPLTYIANEYENIDKIKESLNFKIKVIINGKKEKLGKINALLSAHNPLNILNKGYCIIEDEQKNVISSIEELNKKYKVDIIMKDGTSKVELIHYKK",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. We are looking for a protein with broad substrate specificity for Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-   direction to yield nucleoside 5'-phosphates..2. The protein should have nucleic acid binding and be active in exodeoxyribonuclease VII complex. The designed protein sequence is ",
    "response#1": "LFDAARKKRLPFMPKVIGVVTSPTGAVIRDILHRISDRFPVHVLVWPVKVQGEGAGDEVANAIRGFNALEPGGAIARPDVLIVARGGGSLEDLWSFNDEIVVRAAAESRIPLISAVGHETDWTLIDYAADVRAPTPTGAAEMAVPVKAELEAQATALAARLQGCINRQMDQRRQSVRALMRALPSLDQLLALPRRRFDEAAAGLGRGLELNTINKRRGFERVASHLRPDVLSNRIAERRQLLNDRMARAERTVERLLDRSKSRIDRAEAILASS",
    "response#2": "LFDAARKKRLPFMPKVIGVVTSPTGAVIRDILHRISDRFPVHVLVWPVKVQGEGAGDEVANAIRGFNALEPGGAIARPDVLIVARGGGSLEDLWSFNDEIVVRAAAESRIPLISAVGHETDWTLIDYAADVRAPTPTGAAEMAVPVKAELEAQATALAARLQGCINRQMDQRRQSVRALMRALPSLDQLLALPRRRFDEAAAGLGRGLELNTINKRRGFERVASHLRPDVLSNRIAERRQLLNDRMARAERTVERLLDRSKSRIDRAEAILASV",
    "response#3": "LFDEYNKKEIPRFAKKIGVITSPTGAAVKDIINVSKRRNKGVELLIYPALVQGENAPKDLINGINYFSNRDDIDTIIIARGGGSIEELWAFNNEDLAYAIYNCNKPVISGVGHETDFTICDFVSDRRAPTPSAAAEIGVFNLNEVNTNIENYKNRLYNLIRNTINLKFKELNSLENAIKINSPMNTIANEYIRIDNLKNKLCHKIESKIEYEKIKLSKT"
  },
  {
    "reference": "MTQTHFGYQNVPEEDKVHKVAQVFHSVAARYDVMNDLMSAGLHRIWKAFTVAQAAVRPGFRVLDIAGGTGDLARAFAKQAGATGEVWLTDINESMLRVGRDRMINNGLTNPVLLCDAERLPFPDNYFDRVSVAFGLRNMTHKDAALAEMRRVLKPGGKLLVLEFSKVWEPLKKPYDVYSFSVLPWLGKRIANDADSYRYLAESIRMHPDQDTLKKMMEDAGLDRVQYFNLTAGVAALHTGIKL",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein with demethylmenaquinone methyltransferase activity, 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity is needed to enhance methylation, ubiquinone biosynthetic process, aerobic respiration in a specific context.2. The designed protein should have a binding site that is specific to the target S-adenosyl-L-methionine ligand.3. Ideal protein should have a binding affinity for key enzymes in the menaquinone biosynthesis, menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2, Quinol/quinone metabolism pathway to enhance the overall efficiency of the pathway.4. The protein must exhibit the following characteristics: Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3- methyl-6-methoxy-1,4-benzoquinol (DMQH2).. The designed protein sequence is ",
    "response#1": "MRGEVHGACIAPAKINLTLRVVGQRADGYHLLRSVFMLVSLEDQLTMRELDGGIELQATGIPLDAGEDRCLEA",
    "response#2": "MTAAPPTDTPSATHFGFRTVPVAEKAGLVREVFESVATRYDLMNDLMSGGVHRLWKRHFVDVCAPRPGERILDLAGGTGDIAALLRERVGDGGRIVLGDINGAMLRVGRDRLTDRGLVDGVEYVQCDAEALPFPDNHFDVVTIAFGIRNVPDRARALAEMQRVLKRGARFLCLEFSPVEIPGLRKLYDLYSFRVLPGIGGVVSGDRAAYQYLVESIRRFPDQPTFASMIAAAGFERVTWRNLSGGIAAIHSGWRI",
    "response#3": "MKLSTIEQAVADLKAGKMIILVDDEDRENEGDLAIAAEKVTPEAINFMAKYGRGLICLSLTEDRCDELGLELMVGKNTSSFDTPFTVSIEARHGITTGISAADRAHTVLTAIQDDTKPEDLARPGHIFPLRARKGGVLRRAGQTEGSVDLARLAGLKPAGVICEVMNEDGTMARMPDLEIFAKKHGLKIITIADLISYRLKHESLIRKEVRVLKPGGRLLVLEFPIEIEPNEYNLRYLETKRDKMGHFLKQVDLDLHYEEAKSPYLKTLETKREGYLHTQDNKMGIHFS"
  },
  {
    "reference": "MRARGTHHRSEGDVLRRDRVGKPRERHSAGGTRHPELGTRSQRAGRSRQDDAGAGRQAQVGTGAGDRDARFVATRFVVGTGAADGRADAHGGPRGRCVAVRAAEGRRDERACDARPAAGAIGLRPRCATGDPHAVRRRISGEYRARLRRANPSRRRHPAARGQHRATRDAAHGVGQVTSAARVVDVRVLLDALRDAGLLVSSAPDLPPAVTDVIDDSRRVTPGSAFLAVKGAAQDGHAWLPAAKVSGAALALVEDAAAAVAAGLPAIVVRDGRRAAAVAAAAFHGWPARELTLVGVTGTNGKTTTVGLLRHLLDSPEHPAASIGTLGVLVGSDGVAMPGGSGLTTPGPVELQKLLRELVDRGVRTVTMEVSSHSLDQRRVDGITFAAAVFTNLTRDHLDYHGTMEQYRAAKLRLVGLLARDGVALFNADDPAWRGVANAPTSWTFGTESGADISARDVTFTSDGSRFLLVSPHGAQSVALPLIGDFNIANALGAAAVALAVRMPIADIAQRLSVAPQVPGRLERLRTAPTVLRDYAHTPDALDRALRAVRPFTQQSDGSASRLIVLFGCGGDRDRGKRPEMGRIAEALADVVIVTSDNPRTEDPDRILDDIESGMARQDHVRIVDRRDAIAYALQIATPHDVVVLAGKGHETYQIRGTTSYPFDEREIVNDLMAEMAPVLPNRPPHTA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Incorporating a Meso-diaminopimelate recognition motif motif is critical for the successful design of the protein.2. The ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate binding site should be located in a region of the protein that is accessible to the ligand.3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.4. The protein should exhibit Basic and acidic residues compositional bias.5. The protein have enhanced ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-   muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-   acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-   diaminoheptanedioate.6. The designed protein should be involved in cell division, cell wall organization, cell cycle, peptidoglycan biosynthetic process by localizing to cytoplasm and exhibiting UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, ATP binding, magnesium ion binding.7. The designed protein must have at least one Mur ligase N-terminal catalytic, Mur ligase central, Mur ligase C-terminal domain. The designed protein sequence is ",
    "response#1": "MKVTGLAIDSRRVTPGDLFLAMPGVSGDGRDHIGQAVDKGAVAVVYEGADGYQPVAMPIPQYPVDKLSAIAGLIADRVYGRPTSRMIGVTGTNGKTSITFILEHLLTRAGHRAGVLGTVAYRIGDDVLDAPRTTPESLDLQSLFARFAEAGVTHAAMEVSSHALDQGRVNGTDFRTAVFTNLTQDHLDYHGSMEAYGAAKARLFTWAGLDVAVINADDPWGARMAAVAACPVISYSTTSEDADFRPEPVRMTSKGTAFTVRTPWGQGTVSSDLLGRFNVENVLAAVGAALALGVGLDAVADGVAAAGGVPGRMERFGGQQDKPLVVVDYAHTPDALEKVLQALREHTEDSLWCVFGCGGDRDRGKRPLMGEIAERLADHIILTDDNPRREDPANIVADIRGGMKREHRVVHKRIEAIASAIESARRGDIILLAGKGHETYQVTGAEKTHFDDREVAAAAIASAPVA",
    "response#2": "MKLKELAQLLGMELQGEIELQVDSRAVGEGDGFIAWPGAATDGRRFVQSALAQGAAACLVEADGSEAYINAIHFDSRKVEENDLFVAISGTVVDGHDYIKNAIEQKATAIICEELPQVLVEGVTYVEVDNSNRALAFLASNFYGNPSKKLKLTGITGTNGKTTTAYILEALFDAEGIGVIGTIDYHYDGKKFSAPVTTPESLDLNRLLNEMIEEKIEKCVLEVSSHALHQSRVHDMRFKAGIFTNLSRDHLDFHGDMEHYKNAKKKLFLDNQVQHRIFNIDDTVGQEFYEEFKAGSFTTGIDSNADFKAEDIVLTKSGCQFILKTPFGTKEIHSNLLGRHNVYNLLSAVASAASLGFESKEIKKGIENLESVPGRFECIRSPRGVTAIVDYAHTPDALVNVLQTINEVLRHKGECWTVCGAGGNRDRGKRPIMAQEAVRNSDHVIITSDNPRYEDPQAIIDDMLAGLDAEQRQGVLSIVDRREAIRTACMLAKPGDVVLVAGKGHENYQIIGNEKIHFDDREVARQALEE",
    "response#3": "MTTLRQLIEALPSIATTGDADRLQVDSRAVGEGDGFIAWPGAATDGRRFVQSALAQGAAACLVEADGSEAYGVLVTGTNGKTSTAWWLAQALEGSAFGPCAVAGTLGIGVPPAVDYNGLTTPDPVTLHRALRDLVARGVKACAMEASSIGIAERRLDGLHFDVAVFTNFTQDHLDYHGDMAAYWAAKAELFRWPGLRAAVVNIDDVQGARLAGELLDTALVELWTCSAVGAPARLSAVEAGHGPQGLRFQVVEQGGAEAMVSTTLVGDYNVANLLGVIGLLRAAGLPMADAVAGLAQTPVPGRLERVPAAPGRPTVLVDFAHTPDALAKALAAVREHCAGEVWCVFGCGGDRDRGKRATMGTVAVTGADRVIVTDDNPRSEDPQQIIADVLSGIASLGRLQVVPDRADAIARAIRSAGPGDAVLIAGKGHETYQMLGTEKRPFDDCAVAQAALAARAGGA"
  },
  {
    "reference": "MGGKWSKSSIVGWHAVRERIRQTQQAAVGVGTVSQDLGKHGAITSRNTTFNNADLAWLEAQEDEEVGFPVRPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIYSKKRQEILDLWVYHTQGFFPDWQNYTPGPGTRFPLTFGWCFKLVPLEPEEVEKANEGENNCLLHPICQHGMEDEDKEVLVWRFDSHLAFKHTARELHPEYYKDC",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein's functional design necessitates the inclusion of a Diacidic; necessary for CD4 internalization motif.2. The protein should be optimized for stability and expression in extracellular region where it will participate in suppression by virus of host autophagy.3. The protein must exhibit the following characteristics: Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that leads to activation of PAK2 and induces phosphorylation of host BAD.. The designed protein sequence is ",
    "response#1": "LFLE",
    "response#2": "GHIWS",
    "response#3": "LTPGAV"
  },
  {
    "reference": "MQQPAIPETPQKTNLLNLTPPAAEQALRAFMNERGEQPFRVSQIVRHLWHAPLAEFSEMTTLPAGLREALAEKFSMPRLELSLRQKSADGTEKFLFRLADGEFIETVAIPERDRMTLCISSQAGCALRCAFCATGAMGFSRNLETFEIVGQVRELALLDSPIRATNIVFMGMGEPLMNWKAVDPTLTILNSPAGIGIGARHITMSTVGVLPGIVALGERPEQFRLAISVHAPNDGLRQELMPINTKYPLASVIDAAKVFDRRVTFEYVMLGGVNDKVEHAEQLAELARDCRAFVNLIPLHPGGAGDFRPSTRSEIAAFERKLRSSGVEVAVRKSRGIDIAAACGQLRVERENRRPPVPA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must have a Proton acceptor that is structurally stable and resistant to denaturation.2. The designed protein must be able to perform 4 iron, 4 sulfur cluster binding under specific conditions. The designed protein sequence is ",
    "response#1": "VLFGSDRPYRATGGLAVLRGSFAPDGAIAKAAGFSYDTFTGPARVFEGERDALDALAEGSIKSGCVVVIRQEGPQGGPGMREMLAITGAIKGAGLGASTLLITDGRFSGGTTGNCIAHVAPETMDAGPIALVRDGDSISLDLTARRLDLQVDETELASRRADWVKPPMTSTGVLAKYAALVGSASEGC",
    "response#2": "DLNAIKTAACRKYGLARAPKLVEMIAALPDSERETLLPKLRAKPVRTASGIAVVAVMSKPHRCPHIATTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYNPYVQARSRIDQLKRLGHSVDKVEFILMGGTFMSLPAEYRDFFIRNLHDALSGHTSANVEEAVAYSEHSATKCIGMTIETRPDYCLGPHLRQMLIYGCTRLEIGVQSTYEDVARDTNRGHTVAAVADCFCLAKDAGFKVVAHMMPDLPNVGVERDMESFKEFFESPSFRADGLKIYPTLVIRGTGLYELWKTGRYRNYPPEQLVDIVARILSMVPPWTRVYRVQRDIPMPLVTSGVEKGNLRELALARMDDLGLKCRDVRTREAGIQDIHHKIKPEQVELVRRDYTANEGWETFLSYEDTRQDILVGLLRLRKCGKNVTCPELMGKCSVVRELHVYGTAVPVHGRDADKLQHQGYGTLLMEEAERIARREHRSNKIGVISGVGTRHYYRKLGYELEGPYMVKHY",
    "response#3": "VLFGSDRPYRATGGLAVLRGSFAPDGAIAKAAGFSYDTFTGPARVFEGERDALDALAEGSIKSGCVVVIRQEGPQGGPGMREMLAITGAIKGAGLGASTLLITDGRFSGGTTGNCIAHVAPETMDAGPIALVRDGDSISLDLTARRLDLQVDETELASRRADWVKPPMTSTGVLAKYAALVGSASEGF"
  },
  {
    "reference": "MDYLPLFFNLKGRQALLIGGGDVALRKARLLVRAGARVRVVSREVHAELDELVDAHGGQVVIGEYHPALLDDMALVIAATDDEALNERVHFDAVERSLPVNVVDNPPLCTFVFPAIVDRSPIVIGISSGGQSPVLARLLRAKLETWIPSGYSRLGQLVGGFRDRVKARFNNVNERRKFWEKILQGEVAERVFAGRDEEAAQLLEAELASETPGREVGEVYLVGAGPGDPELLTFKALRLMQQADVVVYDRLVSPQVLDLCRRDADLIYVGKARDNHSVPQPGINALLVEHARKGHRVVRLKGGDPFIFGRGGEEIEELKANGIPFQVVPGITAASACSTYAGIPLTHRDYAQSVKFVTGQLKNRTSDLKFDELVHPNQTIVFYMGLHTLERLCSGLMEHGKPGDTPVAIVSQGTSPDQRVLTGRLDDIVARQAEAQLPAPAIVIMGEVVELQSRLAWFGQTEGAQADA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. We require a protein that can specifically bind to the metabolites produced in the Porphyrin-containing compound metabolism, sirohydrochlorin from precorrin-2: step 1/1, siroheme biosynthesis pathway.2. The designed protein should have NAD binding, precorrin-2 dehydrogenase activity, uroporphyrin-III C-methyltransferase activity, sirohydrochlorin ferrochelatase activity to facilitate methylation, cobalamin biosynthetic process, siroheme biosynthetic process.3. For general function, the protein need meet that Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1; Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.; Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin4. The NAD(+), S-adenosyl-L-methionine binding site should be stable and able to withstand changes in environmental conditions.5. The designed protein is the optimization of the Proton donor, Proton acceptor to achieve high catalytic efficiency for NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin. The designed protein sequence is ",
    "response#1": "VGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHCVPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAASGCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQKLIEHDMPGEMPVAIVENGTAVTQRVIDGTLTQLGKLAQQMNSPSLIIIGRVGEVALVGAGPGDPGLLTLRGLQLMQQADVILYDYLVSDDVLELCRRDAEMICVGKRAGFHSVPQEETNALLVKFASRGKRVVRLKGGDPFMFGRGGEELQVVAEAGIPFQVVPGITSAAGATAYAGIPLTHRDHAQSVMFITGHLKKEKDSLDWSTLARGNQTLVIFMGLMKSDHIRDQLIKHGRAAHTPVAIIENGTRSEQRVLKGTVHDLATLAEHASSPALIVVGEVVQLRTELGLGRAVA",
    "response#2": "MAFPLFADLRGRTVLVVGGGAVALRKAAALLEAGARVRVGAPQLLPALAEWAAQGRIEHLSGHFQPEWLDQAWLAIAATDDGAVNRAVAEAGEARRVWVNVVDDLEASSFHVPARVERGPVQIAISSGGGAPMLARHLRETLETQFDESLGELARLLSRERARIRRHYPDMAVRRRFFDRVLSGPIPSLLREGRLDEAQRGEVALVGAGPGDAGLLTLRGLQVMQQADVVLYDHLVSPEVLDLVRRDAERICVGKRAGAHSVTQEATNQLLVTLAQQGKRVVRLKGGDPFIFGRGGEELQVVAQAGIPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCRPDGDDLDWQTLARGRQTLAIYMGTVKAAAISQQLIAHGRSSTTPVAVIGRGTRVDQQVLIGTLAQLESLAQQAPTPALLVIGEVVNLGDVALIGAGPGDPDLLTFKALRLLQRADVVLYDRLVSQAVLDLARRDAQRIYVGKQRDQHAVAQSDINHTLLELAQQGLKVVRLKGGDPFIFGRGGEELELLAEHDVPFQVVPGITSAAGAASYAGIPLTHRDYAQSVRFVPGYLKQDTPVLTLEDCGSADETLVVYMGLKALDSVVTRLLDAGRAPTTPIALIQNATLPTQLILIGSLEDIVAKAEQQSWDGPTLILVGQVIKLGDVALIGAGPGDPDLLTFKALRLLQRADVVLYDRLVSQAVLDLARRDAQRIYVGKQRDQHAVAQSDINHTLLELAQQGLKVVRLKGGDPFIFGRGGEELELLAEHDVPFQVVPGITSAAGAASYAGIPLTHRDYAQSVRFVPGYLKQDTPVLTLEDCGSADETLVVYMGLKALDSVVTRLLDAGRAPTTPIALIQNATLPTQLILIGSLEDIVAKAEQQSWDGPTLILVGQVIKL",
    "response#3": "MNYFPLFADLRGRTVLVVGGGAVALRKAAALLEAGARVRVGAPQLLPALAEWAAQGRIEHLSGHFQPEWLDQAWLAIAATDDGAVNRAVAEAGEARRVWVNVVDDLEASSFHVPARVERGPVQIAISSGGGAPMLARHLRETLETQFDESLGELARLLSRERARIRRHYPDMAVRRRFFDRVLSGPIPSLLREGRLDEAQRIALVGAGPGDVGLLTLRGLQVMQQADVVLYDHLVSEETLDLVRRDAERICVGKRAGAHSVIQEETNRLLVSLAQQGKRVVRLKGGDPFIFGRGGEELQVAAAAGIPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCRPDGEGLDWNDLARARQTLAIYMGTMKAADISCNLIAHGRGADTPVAVISRGTRTDQQVQIGTLQQVGAGPGDPDLLTLKALRLMYAADVVLYDRLVSPEIMKRVRPDADKVFVGKEAKHHPVPQEQINDMLVRLAREGKKVLRLKGGDPFIFGRGGEEIDQLAAAGLPFQVVPGVTAASGCAAYAGIPLTHRDFSQSVRFVTGHMKNDEPDLDWPVLAKEDQTLVIYMGLITLPVICEQLQAYGMNSAMPVAIVEQGTLPQQRVVTGTLQDIAGKVEAAGVKAPAIIIVGQVGSVALVGAGPGDAGLMTLRGLRVLNEADVILHDRLVSADVLRLARRDAELIEVGKQAGNHHTTQDRIHELMLEHARAGKRVVRLKGGDPFVFGRGGEELEVLADAGIAFEVVPGITAAVACAAYAGVPLTHREHAQSLRLVTAHLKDSTDTLDWQALAQEKQTLAVYMGVSGLDLLRDKLIQHGRAASTPFALIENGSRREQRVVTGTLADLAERARGHQVQSPALLIVGEVAAL"
  },
  {
    "reference": "MSGPELGRLIDEVDRTVDVQFAAAGDLDELNRLYASLLGRRGSLNALMKQLPGAAPEERKALGQRLNAVKGRVERAREEATARIKRAARDRELSAPPLDITLPGRWRAPGRPHPIMRTLDEIVDIFVGLGFDVAEGPQIELARYNFDLLGFPADHPAMDMHDTFYMAGDPGQNVLLRTHTSPVQVREMLSHPPPVMIVAPGVVYRRDDDASHSPMFVQIEGLVVDRDVSLADLKGLLEVYSRRMFGEATRTRFRPSYFPFTEPSAELDVSCLVCFGENRACPQCKGTGWLEVLGCGMVHPTVLRNVGIDPEEYTGLAFGIGVDRTANMKFAVDDIRAFYENDVRFLGSL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should have a binding site that is specific to the target Mg(2+) ligand.2. The protein design must be able to perform ATP binding, magnesium ion binding in order to contribute to phenylalanyl-tRNA aminoacylation. The designed protein sequence is ",
    "response#1": "ERPYVTGAIVRGLDMDEAALESLIQLQEKLHATMGRKRAKGAIGVHDLTMLKGDSVTDETGKSITYTSADPDEATFVPLDADAEMTPSEVMASHETGQTYGDLVADFDRVPAIYDAIGLFSFPPVINGRRTEVSVDSRDLFIEMTGTNQWTIDHMCNIVCYALAARGR",
    "response#2": "ERPYVTGAIVRGLDMDEAALESLIQLQEKLHATMGRKRAKGAIGVHDLTMLKGDSVTDETGKSITYTSADPDEATFVPLDADAEMTPSEVMASHETGQTYGDLVADFDRVPAIYDAIGLFSFPPVINGRRTEVSVDSRDLFIEMTGTNQWTIDHMCNIVCYALAARGS",
    "response#3": "IDVTMFSSSSESGALHPVMQTMDRIVEYFSSMNFTVKTGTMVEDDFNNFEALNLPKYHPARDMQDTFYFKDEMLLRTHTSPVQIRTMMSSKPPIRMIAPGAVFRRDYDITHTPMFHQVEGLLVDKEGAVSFANLKFILEDFLKYMFGDVEVRFRPSFFPFTEPSAEVDISCVFCKGSGCRVCSKTGWLEVLGCGIVDPNVFEAVKYQNVSGYAFGLGVERFAMLIHQIGDLRSLFEGDIKLLEQS"
  },
  {
    "reference": "MNSSPKYVFVTGGVSSSLGKGIISASLAKLLQSRGLNVTIQXFDPYINIDPGTLNPYEHGECYVTDDGTETDLDLGHYERFLDIKTSNSNNATTGQIYQTVINKERKGDFLGKTVQVIPHITNEIKRRIQLIESEKEYDIIITELGGTVGDIESYPFIEAVRQFRWEVGIANSIVIHLTLLPFLAAAGELKTKPTQHSVKKLMESGVNADIIVCRTEHEMNEELRSKIALFCNVDVESVIQSIDASTIYEVPILMQEEKLDQVVLKKLNIKNSNKTEFKKWKTFVQKVKNPNDTVDIGLIGKYVELQDSYKSILESFVHAGAENELKVNVIPIHSEEINKSNLIELLSSLDGILVAPGFGERGIEGKICAIQFARENNIPFLGICLGMQMAVIEYSRNVLNLSDANSTEMNTETSNPVISLMENQKNITEMGGTMRLGSWKCDLIENSLAQRIYSKSKIKERHRHRYELNFEYMDSLKSKGLVFSGLNPDTNLVEIIELENHPWFLGVQFHPEYKSTALNPHPIFISFVEASYKKKINNGRK",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should contain one or more CTP synthase N-terminal, Glutamine amidotransferase domains that are essential for its function.2. The protein should have a high affinity for ATP, Mg(2+), CTP, L-glutamine, UTP.3. For general function, the protein need meet that Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen4. A protein with glutaminase activity, ATP binding functions is required to enable glutamine metabolic process.5. We require a protein that can specifically bind to the metabolites produced in the CTP biosynthesis via de novo pathway, CTP from UDP: step 2/2 pathway. The designed protein sequence is ",
    "response#1": "MEARGLRVTNIKLDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTLARMTRQNNFTTGRIYEAVISKERRGEYLGATVQVIPHVTDEIKLRIQSAAEGADVAIVEIGGTVGDIESLPFIEAIRQLKIESGSQNALSVHVTLVPFIATAGEMKTKPTQHAVKEMRAIGIQPDVLLCRCDRALPRDMKEKIALFSNVPVDRVVAAMDVACIYELPLALHAEGIDEKLTVPFAGPDLKPWQALSDAYLSVMESLKIAGYEFHKKIEFVWVNSRFLNKDNIDSELEGAKGILVPGGFGEEGVEGKILAANFARTKDIPYLGICLGMQVACIEFARNVLGLSEAHSTEINPTTNNKIFDLMEGKDRENIGGTLRLGEYQADLKPGSLANKLYNSNIAIERHRHRYEFNNDFIKQFEDNGMIFSGKYLEKNLVEIVEIPNHKFFIASQYHPEFTSRPIKPNPLFKGFVDSYKSLKEALLHGGFAHRLKVNTHWIEAQDLRWPECAQQLAQFDGILVPGGFGKRGVDGMLQAIRYSRENKVPYFGICLGMQTMVIEYARNVCGLAQADSTEFDNTTAQRVIYKLRELKGVDELGGTMRLGAYPCNLTEGSTARAAYGKSSISERHRHRFEFNRDYEKVLTDGGLRLTGQTPDGMYVEICEIENHPWYLGCQFHPEFKSKPLEPHPLFASFVEAYAARVLKDAYKSLQEALVHGGIANNVRVNITWLASEIFEQDHEAILALEDVNAILVPGGFGERGTSGKIAAVQFARERKVPYLGICYGMQMAVIEAARNLAGLKDANSTEIGPTRNPVVGLMTEWVRGNRTELRQEGGDLGGTMRLGAYDAVLSEGSRVAEIYGTRLISERHRHRYEVNPAYRRHLEEHGVLFSGMSPDGILPEVIEIKDHPWFIGVQFHPELKSKPLDPHPLFSSFV",
    "response#2": "MEARGLRVTNIKLDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTLARMTRQNNFTTGRIYEAVISKERRGEYLGATVQVIPHVTDEIKLRIQSAAEGADVAIVEIGGTVGDIESLPFIEAIRQLKIESGSQNALSVHVTLVPFIATAGEMKTKPTQHAVKEMRAIGIQPDVLLCRCDRALPRDMKEKIALFSNVPVDRVVAAMDVACIYELPLALHAEGIDEKLTVRISLVGKYTQLHDAYLSVMESLKHAGWENQSRVSISWIDSECVTPDSVDDLLAGSDGILIPGGFGDRGIEGMIVAARYARENKIPYFGICLGMQIAVIEYARDVMGLVDANSSEFAPNGLHSVIDLMPDQMGNIPKGGTMRLGEYPCAIVPETIMNRAYRGEAEIKERHRHRYEFNNVFREQLVKAGLTISGHSPDGHLVEAVELPEHVHPFYLGVQFHPEFKSRPNRAHPVFREFIGAAAARQTARPG",
    "response#3": "MEARGLRVTNIKLDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTLARMTRQNNFTTGRIYEAVISKERRGEYLGATVQVIPHVTDEIKLRIQSAAEGADVAIVEIGGTVGDIESLPFIEAIRQLKIESGSQNALSVHVTLVPFIATAGEMKTKPTQHAVKEMRAIGIQPDVLLCRCDRALPRDMKEKIALFSNVPVDRVVAAMDVACIYELPLALHAEGIDEKLTVPVARDRSDWEAMVDAVHHPDGEVTIGLIGKYVELQDSYKSILESFIHAGAENEVKVKIESIHSEHITPDVIKNKIAKLDGVLVAPGFGERGIEGKIKAVQYAREHKIPFLGICLGMQMAVIEYGRNVCGLEKANSTEMDANTPHPVIGLMDEQKNITEMGGTMRLGAWACKLEPNSIVSDVYGEEVIMERHRHRYEFNDAYREQLEKAGMKITGTNPDTGLVEIIEIEDHPWFVGVQYHPEYKSTVSNPHPLFVAFVRAAREHKK"
  },
  {
    "reference": "MASKRISRELRDMQRHPPANCSAGPVAEEDIFHWQATIMGPHDSPYSGGVFTVSIDFSSDYPFKPPKVNFKTKVYHPNIDSKGSICLDILKEQWSPAPTTSKVLLSICSLLTDPNPNDPLVPEIAHLYKVDKSKYESTAQKWTQKYAMG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. We require a protein that can specifically bind to the metabolites produced in the Protein modification, protein ubiquitination pathway. The designed protein sequence is ",
    "response#1": "CTAILKFADAFGHRKLRSQAQSYIAQNFKQLSHMGSIREETLADLTLAQLLAVLRLDSLDVESEQTVCHVAVQWLEAAPKERGPSAAEVFKCVRWMHFTEEDQDYLC",
    "response#2": "LDLTNCTAILKFADAFGHRKLRSQAQSYIAQNFKQLSHMGSIREETLADLTLAQLLAVLRLDSLDVESEQTVCHVAVQWLEAAPKERGPSAAEVFKCVRWMHR",
    "response#3": "CTAILKFADAFGHRKLRSQAQSYIAQNFKQLSHMGSIREETLADLTLAQLLAVLRLDSLDVESEQTVCHVAVQWLEAAPKERGPSAAEVFKCVRWMHFTEEDQDYLC"
  },
  {
    "reference": "MKRTDYCGALRETDVGRAAVVMGWAQNKRDMGGVIFIDLRDREGTLQVVFDAQNLSREDFAAAEGLRVESVVAVRGELRPRSEETRNPRLATGAFELCARGMEVLSEARPLPFPLEDAAGVREEVRLRYRFLDLRRPGLLENLRFRARVARLVSDYLESEGFLAVETPMLTKSTPEGARDYLVPSRVHQGEFYALPQSPQIFKQLLMVAGIDRYYQIARCFRDEDLRADRQPEFTQVDMELSFVDQEDILRHLERMFRHLFREALGIELPAPFPRLTWREAMDRYGSDKPDLRFGLPIADVTELARGCGFSVFRKAVEAGGVVRALNVKGRCDFSRGTIEELTKKALAYGAGGMAWIALREDGEPYSVLTKYFSKADFAALLDAVDGRPGDFILFCADRFSTVCRTLGGLRLDLADLLGLRRDSGDYKFCFVTDFPQFEYSEEEGRYVATHHPFTMPYPEDLPYLLTDPARVRAQAYDVVLNGVELGSGSIRIHSRDVQKRMFEALGFSDGQIGERFGFLVNAFQYGTPPHGGFAFGLDRLVMQMLGAPSLRDVIAFPKTKDARCPLTGAPGPVDPAQLDALGLAGLLREGVGVPAKARRSQTRVDVDAAANLARLRVAPEEREALERQMAELVAFAGQLAEADTEGVPPAGHVVPLENVFRPDRAGTPLPRADLLAGAPETWDGCFFVPQVVE",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The presence of Aminoacyl-transfer RNA synthetases class-II family profile domains is critical for the interaction of the designed protein with its intended targets.2. For general function, the protein need meet that Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp- AMP and then transferred to the acceptor end of tRNA(Asp).3. The protein should be designed to catalyze the following reaction ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-   aspartyl-tRNA(Asp).4. The protein should have a high affinity for ATP, L-aspartate.5. A protein with glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity, aspartate-tRNA ligase activity, asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity, transferase activity functions is required to enable aspartyl-tRNA aminoacylation, regulation of translational fidelity. The designed protein sequence is ",
    "response#1": "MFTAINKELNDTAFVAKTLLNNLKTEEYAVIENSADLVNIFGALQSGSITQILLQNAIKELLQNASFDYKKYFTENSISDDTLAETIETVIKENQGTADEIKAGNQGKAGILVGKVLQVVGKGANGKVVREMILTQLNS",
    "response#2": "MFTAINKELNDTAFVAKTLLNNLKTEEYAVIENSADLVNIFGALQSGSITQILLQNAIKELLQNASFDYKKYFTENSISDDTLAETIETVIKENQGTADEIKAGNQGKAGILVGKVLQVVGKGANGKVVREMILTQLGKLVVSREKRGKVVLAGWVARRRDLGGVVFLDLRDASGIVQVVAREGVAGQVASRVRAEFCLRVAGEVRLRPAGNENPELETGDVEVVADEIEVLPMPDEDALPDLESLRELNEETRLKYRYLDLRRPGPARALRLRSEMNRLAREVMARHRFVEVETPYLTRSTPEGARDFLVPARLQPGHWYALPQSPQLFKQLLMVAGLERYYQLARCFRDEDFRADRQPEFTQLDIEMSFVTEDDVMALAEDVIATLWSQLAGVELALPLPRLTYAEAMRRFGSDKPDLRFGQEIVDLTKFFAGTSLRIFQAPYVGAVVMPGGAGQSRSELDEWTTFARSRGAKGLAYVLVGAELTGPIAKNLSDAERAGLPAATGARPGDAIFFAAGEQRASQELLGAVRLEIARRCRLTDPASGAPAWSLCWIVDPPLFEPDGAGGWTSVHHPFTAPKPEWMDRFADKPGEALAAAYDIVANGNEIGGGSIRIHRADVQQRVFEVLGISEAEANAKFGFLLEAFRYGPPPHGGIAFGWDRIAALLAGVESIRDVIAFPKTTKARPL",
    "response#3": "MFTAINKELNDTAFVAKTLLNNLKTEEYAVIENSADLVNIFGALQSGSITQILLQNAIKELLQNASFDYKKYFTENSISDDTLAETIETVIKENQGTADEIKAGNQGKAGILVGKVLQVVGKGANGKVVREMILTQIPKGVLEKAPALLKRDRIVHSAREVLRSYNFVEIETPFLTRSTPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMIGGMDRYYQIVRCFRDEDLRPNRQPEFTQLDLEASFIDEEFIYEIVEQLLASMFAIGDISLPTPFPRITYDEAMDLYGTDRPDTRFGMRFKEVTDIVRNTSYAIFQNIISQGGVVKGFCVRGKANELSKNVLQNEYAMKIVPSFGAKGMTWMKVIDGKLQSNIVQFFAHKEQQRLLERFEAREGDVLFMIADVSKNLVNEVLGKLRLHLAERLGLIPSGQYMPLWVTHFPLFEMKEDGIGSQHHPFTQPEREDFDPDNLDELLALKSRAYDIVINGEELGGGSIRIHRMETQRKVFKALGLSDSEAEAKFGFFLKALDYGAPPHGGLALGMDRVISMIIGTESIRDVIAFPKTPKALDAILFKEPANNPHGRIAVLRLPKGAELSRKAIDDYTQFVGIYGAKGLAYIKVENIDNGTGGLHSPILKFLPENVIAEILKRTQAQSGDILFFGADKAKIVNESV"
  },
  {
    "reference": "MTKMHRYAAIAALAIFLSGCMAQRQPAPVEEVKPAPEQPAQPPQPPVVPSVPTIPQQPGPIEHEDQTGQPAPKVRHYDWNGAMQPLVSKMLQADGVTAGSVLLVDSVNNRTNGSLNANEATETLRNALANNGKFTLVSVQQLSMAKQQLGLSPQDSLGTRSKAIGIARNVGAQYVLYSSASGNVNAPALQMQLMLVQTGEIIWSGKGAVQQQ",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Prioritize Pro residues compositional bias within the protein sequence to achieve desired characteristics.2. The design objective is to achieve a protein with helix-strand-strand-helix-helix-helix-helix-strand-strand-turn-strand characteristics.3. A protein that can perform enzyme regulator activity in periplasmic side of cell outer membrane to support peptidoglycan biosynthetic process. The designed protein sequence is ",
    "response#1": "NFLGTGAAVSIAGTKNDYGTSVNLGYTEPYFTKDGVSLGGNVFFENYDNSKSDTSSNYKRTTYGSNVTLGFPVNENNSYYVGLGHTYNKISNFALEYNRNLYIQSMKFKGNGIKTNDFDFSFGWNYNSLNRGYFPTKGVKASLGGRVTIPGSDNKYYKLSADVQGFYPLDRDHLWVVSAKASAGYANGFGNKRLPFYQTYTAGGIGSLRGFAYGSIGPNAIYAEYGNGSGTGTFKKISSDVIGGNAIATASAELIVPTPFVSDKSQNTVRTSLFVDAASVWNTKWKSDKNGLESDVLKRLPDYGKSSRIRASTGVGFQWQSPIN",
    "response#2": "NFLGTGAAVSIAGTKNDYGTSVNLGYTEPYFTKDGVSLGGNVFFENYDNSKSDTSSNYKRTTYGSNVTLGFPVNENNSYYVGLGHTYNKISNFALEYNRNLYIQSMKFKGNGIKTNDFDFSFGWNYNSLNRGYFPTKGVKASLGGRVTIPGSDNKYYKLSADVQGFYPLDRDHLWVVSAKASAGYANGFGNKRLPFYQTYTAGGIGSLRGFAYGSIGPNAIYAEYGNGSGTGTFKKISSDVIGGNAIATASAELIVPTPFVSDKSQNTVRTSLFVDAASVWNTKWKSDKNGLESDVLKRLPDYGKSSRIRASTGVGFQWQSPIN",
    "response#3": "ALTDPDNPRWISAQKDIISYVDETEAATSTITKNQDAQNNWLTQQANLSPAPKGFIIAPENGSGVGTAVNTIADKGIPIVAYDRLITGSDKYDWYVSFDNEKVGELQGLSLAAGLLGKEDGAFDSIDQMNEYLKSHMPQETISFYTIAGSQDDNNSQYFYNGAMKVLKELMKNSQNKIIDLSPEGENAVYVPGWNYGTAGQRIQSFLTINKDPAGGNKIKAVGSKPASIFKGFLAPNDGMAEQAITKLKLEGFDTQKIFVTGQDYNDKAKTFIKDGDQNMTIYKPDKVLGKVAVEVLRVLIAKP"
  },
  {
    "reference": "MSLKLEVKTEIKLDYQGFQNQINEFHNRINDKNSPDINFLGWNNFPEVAINPQEIARMRKIVENLHQNSINVLVVIGIGGSYLGAKAALDFILGLGPFENKPEVIFLGNSLSSTDLYQKIEYLKTKNFAINVISKSGSTIEPAITFQILYQFLIDQIGEKLAKTRTFVTTSIKSGELLEIAKSNELEIFEVIESIGGRFSVLSSVGFFPLLFAKINVDEIIQGAIEAHKRYSTSSISQNLAYKYALFRFLMYKNFNYKTEILISYEPFLIYFNEWWKQLFGESEGKNLKGLFPASAIFTTDLHSLGQFIQDGSKNFFQTIIYIKKPKFDLGIKKIVQFNTKINKLSGKTVSEINFQAFLATTLAHSSYGNNPNLVLEIADSSPKTFGHLVMFFEKACAMSAYLLGVNPFDQPGVESYKNELAKNLGWDR",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The protein can be expressed in cytoplasm and perform carbohydrate derivative binding without triggering undesirable cellular responses.2. Ideal protein should have a binding affinity for key enzymes in the Carbohydrate degradation pathway to enhance the overall efficiency of the pathway.3. The designed protein must have a active site, Proton donor that is structurally stable and resistant to denaturation. The designed protein sequence is ",
    "response#1": "VLLKNEKNALPLKKPKMLSVYGYDATVPRTKNTDVLFTLGYYSSAEMAQAVLGTEQHFDQAAKGGTIISGGRAGANGPPYISDPLSAIQHRAADDDTWVNWDLDSGNPDVNSGSDACLVFINAIATEGWDRDGLHDDFSDGLVLNVASKCENTIVVIHNAGVRLVDQWVDHPNVTALVMAHLPGQDSGAALVKLLYGEEYFSGKLPYTIPHNESDYGHVYRSCAHDRAQDDKDPQCDFTEGVYIDR",
    "response#2": "LGWPGLCLHDAGNGVRATDLVSAYPSGLHVGASWDKNLTYERGLYMGKEFKAKGVNVLLGPNAGALGRVPVAGRNWEGFTIDPYLSGILNAESIVGHQEAGVIATTKHFIGNEQELYRRPYFGVEAVSSNIDDKTVHELYMWPFMDSVKAGAASAMCSYQRVNNTYACMNSKLMNGLLKGELEFEGFIMLDWNAVHTVDSAEAGLDMVMPRGNWGTNLTAAINNGTTSKERLVDMATRILQA",
    "response#3": "VLLKNEKNALPLKKPKMLSVYGYDATVPRTKNTDVLFTLGYYSSAEMAQAVLGTEQHFDQAAKGGTIISGGRAGANGPPYISDPLSAIQHRAADDDTWVNWDLDSGNPDVNSGSDACLVFINAIATEGWDRDGLHDDFSDGLVLNVASKCENTIVVIHNAGVRLVDQWVDHPNVTALVMAHLPGQDSGAALVKLLYGEEYFSGKLPYTIPHNESDYGHVYRSCAHDRAQDDKDPQCDFTEGVYIDS"
  },
  {
    "reference": "MWSHLNRLLFWSIFSSVTCRKAVLDCEAMKTNEFPSPCLDSKTKVVMKGQNVSMFCSHKNKSLQITYSLFRRKTHLGTQDGKGEPAIFNLSITEAHESGPYKCKAQVTSCSKYSRDFSFTIVDPVTSPVLNIMVIQTETDRHITLHCLSVNGSLPINYTFFENHVAISPAISKYDREPAEFNLTKKNPGEEEEYRCEAKNRLPNYATYSHPVTMPSTGGDSCPFCLKLLLPGLLLLLVVIILILAFWVLPKYKTRKAMRNNVPRDRGDTAMEVGIYANILEKQAKEESVPEVGSRPCVSTAQDEAKHSQELQYATPVFQEVAPREQEACDSYKSGYVYSELNF",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. For general function, the protein need meet that Immunoglobulin-like receptor which plays an inhibitory role in degranulation of mast cells2. Ensure the presence of a signal peptide within the protein.3. The designed protein should contain Extracellular, Cytoplasmic topological domains.4. The protein's functional design necessitates the inclusion of a ITIM motif motif.5. The protein should contain Helical transmembrane regions to facilitate transport across the membrane.6. The protein should be able to modulate cell-cell adhesion via plasma-membrane adhesion molecules, negative regulation of mast cell activation, cell surface receptor signaling pathway in a way that leads to a desirable outcome. The designed protein sequence is ",
    "response#1": "VMYILMQPPNIKGVMLECHSGGWFPQPHMEWRDNKGNIIPATSKAHSQDENKLFNMTMTLLIEASSHRSITCYLQNLLTHQC",
    "response#2": "DIVFLIDGSGSISSRNFATMMNFVRAVISQFQRPSTQFSLMQFSNKFQTHFTFEEFRRSSNPLSLLASVHQLQGFTYTATAIQNVVHRLFHASYGARRDAAKILIVITDGKKEGDSLDYKDVIPMADAAGIIRYAIGVGLAFQNRNSWKELNDIASKPSQEHIFKVEDFDALKDIQNC",
    "response#3": "VMYILMQPPNIKGVMLECHSGGWFPQPHMEWRDNKGNIIPATSKAHSQDENKLFNMTMTLLIEASSHRSITCYLQNLLTHQC"
  },
  {
    "reference": "SLAICTTDTRPKTAAASFKMAGKRIHLVGCAKGAGMIHPNMATTLAFVVTDAAIAPALLQKTLNDVTRRTFNSISVDGDTSTNDTLLVLANGEAGAPIITAGSKAHRAFILALEEVCQSLALQIVADGEGAQRVIEIEVRHAKSEAGARRIAETIATSPLVKTAFAGGDPNWGRVFAAAGRSGVKFDPALVDIELAGIPVLRRGQPLAFNERAASNKLLADHVALVVDLHAGDAAARYWTCDFTSEYVRINASYRT",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The ideal protein would be one that plays a critical role in arginine biosynthetic process through methione N-acyltransferase activity within cytoplasm.2. Ideal protein should have a binding affinity for key enzymes in the Amino-acid biosynthesis, N(2)-acetyl- L-ornithine from L-glutamate: step 1/4, L-arginine biosynthesis pathway to enhance the overall efficiency of the pathway.3. The designed protein must have a Nucleophile active site that is highly conserved among related enzymes.4. For general function, the protein need meet that Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate. The designed protein sequence is ",
    "response#1": "MIKPTNTRKVAGLADVERLVGDRQTENVLRFLESAFFAQNLTVTLDRSIVQSGNVNTYGDLFIHKDLVRAVKAAQRFSLWPVGRISDDVILDDAELRAGGGIDFQTGTCHATIALGDTPVLISSFGPRVFRNGDILTHELTLPDGTRTRIYPTAVHYRNQPGGWEGDREIHTRYLTRAGLGLIQEGDWYLSEHVEHF",
    "response#2": "MHLDTLRRLADPSGPLVADFERAGLLARDPHAPDMVFFQGRPAGTKGSIFTTNKVMAAAPEKVAEYNRRWGLPIPVAYSPGKVDDPRVLRELPDAPDVVHVTGEPRRPLARDAYAQLRDQGVRVVFRPETAADIGPGRVLTRDGAVEAVDVLVHSTGYRHTLPWLAPLGLVDERGRVRPGEGAGAGDGLPGLYFLGLFQPIGANMPLRRGCEAQADYIAALLREP",
    "response#3": "MASVSKSLILVSGFGPFGHVKVNPSREVVEKLDNLDLKDVNLKYELIPVNWEKVGPAVSRLHEKHKPEFVVHIGLSLKRSTFTPERRGHNDGYIPDDEGQCPLDEGCIIEGPRILDNQPTSDIVRNIRENGWDARLSEDAGRFVCNHVFYGLMHQQVHKFGNAKAGFMHIPYLPKQVDEGEENVPVMPLDVIVKGIELAIEVIVENFKLH"
  },
  {
    "reference": "MTEINTVLAELKRGVDEILSEADLIEKLKENRPLRVKLGADPTAPDIHLGHTVVLNKLRQFQQFGHEVIFLIGDFTGMVGDPSGKNTTRPPLSRDDVLRNAETYKQQIYKILDPQKTRIVFNSSWLGELGTEGMIRLTSNYTVARMLERDDFKKRFSNNQPIAIHEFIYPLLQGYDSVALDADIELGGTDQKFNLLVGRELQKSAGQKPQVAITLPLLVGLDGEKKMSKSLGNYIGVAESPTEMFGKVMSISDELMWDWYDLLSFRPLTEIAQLKQEVENGRNPRDIKVLLAKELIARFHSEADAEAAEQEFINRFQKGAMPEDMPELTFEGEMGLANLLKEAGLVASTSEANRMVQQGGVKIDGEKVEDAKLVIKASTAVYQVGKRKFARVTVK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein must be able to regulate tyrosyl-tRNA aminoacylation. The designed protein sequence is ",
    "response#1": "RLGVLTGLGKSGQHSLEVPGCLLHTRCATVPHLTQDTLLTLSDLPAVTQVSVDSLAEHHEVLEEFKEGVRKFAGLHDTVIFCSLHDSASPSPAGHVTNKTVSVWGSGGRIELTAARFMSIQAAVQPDCYQSMADGETWQANTSRKRVRKAVDRTLAHLDECLVLHQKTQELKHAEIFGVVEGGDILEERLRSARETAKRPVGGFVLDGFHSSAMDQDVRAQLIQETSAELPQEKPRLVLGVGRPDEVISCVEAGVDLFESFFPFQVTERGCALSFNYTIDPDPETAGTSASTVLECNGETPEVKKPSANEDVENMTPFEINLKDKRYRDDFRPLVEGCVCYCCQKHMRAYVHHLLVTNELLAGVLLMLHNMAHYLGFFKALRDAITSDRLQDFKNTVLHRR",
    "response#2": "SGGRIELTAARFMSIQAAVQPDCYQSMADGETWQANTSRKRVRKAVDRTLAHLDECLVLHQKTQELKHAEIFGVVEGGDILEERLRSARETAKRPVGGFVLDGFHSSAMDQDVRAQLIQETSAELPQEKPRLVLGVGRPDEVISCVEAGVDLFESFFPFQVTERGCALSFNYTIDPDPETAGTSASTVLECNGETPEVKKPSANEDVENMTPFEINLKDKRYRDDFRPLVEGCVCYCCQKHMRAYVHHLLVTNELLAGVLLMLHNMAHYLGFFKALRDAITSDRLQDFKNTVLHV",
    "response#3": "RLGVLTGLGKSGQHSLEVPGCLLHTRCATVPHLTQDTLLTLSDLPAVTQVSVDSLAEHHEVLEEFKEGVRKFAGLHDTVIFCSLHDSASPSPAGHVTNKTVSVWGSGGRIELTAARFMSIQAAVQPDCYQSMADGETWQANTSRKRVRKAVDRTLAHLDECLVLHQKTQELKHAEIFGVVEGGDILEERLRSARETAKRPVGGFVLDGFHSSAMDQDVRAQLIQETSAELPQEKPRLVLGVGRPDEVISCVEAGVDLFESFFPFQVTERGCALSFNYTIDPDPETAGTSASTVLECNGETPEVKKPSANEDVENMTPFEINLKDKRYRDDFRPLVEGCVCYCCQKHMRAYVHHLLVTNELLAGVLLMLHNMAHYLGFFKALRDAITSDRLQDFKNTVLHRC"
  },
  {
    "reference": "MTKFVFVTGGVVSSLGKGIASASLAAILESRGLKVTLIKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIETRMKRTNNFTTGRIYQSVLEKERRGDYLGKTVQVIPHVTNEIQEYIKRGAGMGTADAVDVAIVEIGGTVGDIESLPFLEAVRQMSLRMGPNNSAFVHLTYVPWIAAAGELKTKPTQHTVQKLREIGIQPDALLCRADRRIPDDERAKISLFTNVPEWGVISMWDVATIYRVPRMLHEQGLDGLICDKLRLNTPPTSLRRWDELVFETENPQHEVAIAMVGKYVDLSDSYKSLNEALRHAGLKNHAKVRIEYIDSETITSDNVDSLGKYDAVLVPGGFGQRGVEGKINAARYAREHKTPYLGICLGMQVATIEYARHVAGLKKANSTEFEPETPEPVIALITEWKDEDGTIKTRNASSDLGGTMRLGAQSSDVAKGTLAHAIYGDVVTERHRHRYEANVNYLDQLRKAGLVISALTQREQLTEIVELPREVHPWYMGVQFHPEFKSTPWNGHPLFNAFIKAALQHQAGAPRSTTLPDATEATA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. A protein that can modulate the overall flux of metabolites through the Pyrimidine metabolism pathway, enhancing or suppressing its activity as needed.2. The protein contains novel Glutamine amidotransferase domains that confer a unique function or activity.3. The protein must exhibit CTP synthase activity.4. For general function, the protein need meet that Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.5. The designed protein should be able to compete with endogenous proteins or inhibitors for L-glutamine, Mg(2+), UTP binding. The designed protein sequence is ",
    "response#1": "MPDAYLSLLESLTHAGIANDARVNIKWVNAEELAEGDLETQLGDADGILVPGGFGIRGIEGKIKAAEYARTHNVPYLGICLGMQIAVIEYARHVAGLTGANSAEFDPYAPHKVIDLMPEQLEVEGLGGTMRLGDWPMELRAGTKIAELYGVPQGGTVRERHRHRYEVNPAYVGQLQDAGLVISGVTPGVQGRGAGLVESIEIPGHPFFVALQAHPEFKSRPMRPSPPFAGFVETGLVS",
    "response#2": "LQDSYKSILESFIHAGAENEVKVKVQSIHSEYISEKNVAKKFAHLDAVLVAPGFGERGIEGKIAAVKYVRENNIPFLGICLGMQMAVIEFSRNVLGIADANSSEMDENTNNPVIDLMASQKMVTDKGGTMRLGAWECELVEGSIAYKVYQNKTIKERHRHRYEFNNKYKAQIEEAGMKATGINPETGLVEIIEIPANNWFVGVQYHPEYKSTVANPHPLFVAFVGQ",
    "response#3": "LPDAYLSVTAALRAGGFAHRTTVNLKWIASDDCITAEGAAEQLGDVDGICVPGGFGVRGIEGKVGALKFAREQGIPTLGLCLGMQCMVIEYARHEAALPEASSTEFDPETSTPVIATMAAQVDIIATGELGGTMRLGLYPASFTTGSLAADLYGAVTASERHRHRYEVNNTYRDQIAAAGLAFSGTSPDGTLVEYVELDRGVHPFYIGTQAHPELRSRPTNPHPLFAGLG"
  },
  {
    "reference": "MSLEIVILAAGKGTRMKSSQPKVLHKLAGKSLLQWAIEAALQVGAEKIHVVVGHGADQVKQAIDIPQVNWVEQREQNGTGHAVAQALPHISKDATVAVLYGDVPLISAATLQDLLNKVSDQSLALLTASLDNPNGYGRIVRNNNGDVIRIVEQKDAGAEQLTINEVNTGILAASASSFNRWLPALKSDNAQGEYYLTDTIAMAVSEGYQIAVAQPTSELEIQGVNSRVQLQELERALQLRRAQQLMEAGVTLADAGRIDIRGTLSCASDVSIDVNCVFEGNVEIAENCKIGPNCVLVDCKIEEGVEVKANSMIEDSHLAANCIIGPFARLRPGTVLAEEVRIGNFVEVKKSTFAKGSKANHLSYIGDSELGSGVNVGAGTITCNYDGVNKHQTKVGDNAFIGSNTALVAPVNVGVGATIGAGSTITGNIADGELAVARGKQRNITGWKRPTKKQP",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The Proton acceptor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.2. The designed protein should be involved in UDP-N-acetylglucosamine biosynthetic process, cell wall organization by localizing to cytoplasm and exhibiting UDP-N-acetylglucosamine diphosphorylase activity.3. For general function, the protein need meet that Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc)4. The protein should be optimized for activity in UDP-N-acetyl-alpha-D- glucosamine biosynthesis, Nucleotide-sugar biosynthesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.5. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.6. The designed protein should have a binding site that is specific to the target Mg(2+) ligand. The designed protein sequence is ",
    "response#1": "AVIMAAGKGTRMHSKLPKVMHSLAGKPLIEHVLDVANQVGVERPFVIVGHGREIVAARVQERAETVVQTEQKGTGHAIMQAMPYLEDAQTVLVLSGDQPLLKPESLQTLIKLHQDRGACATVLTLLTDTLPDPTGYGRIIRDADGNVSRIVEQKDASADELAIRETNAGIYCFNAKVLAQALPRLSADNAQKEYYLTDVIGLAIADGESVAVHLATEDEVAGVNNRVQLAQLGAELNRRTVERWMLAGVTVQDPATTWIDIKVTLAPDVTILPGTQLKGATTVATGAIVGPDTTLVDCEIGEGATVKRTDGTLAVIGAGASVGPFSYLRPGTVLAADGKIGTFVETKNATIGAGSKVPHLSYIGDTEVGEGANVGAGTITANYDGVNKHRTTVGSFVRTGSHNVFVAPVTIGDGAYTGAGTVVRKDVPSGALALNVAPQRNIEEWVKKKRPGTAAADAARRAREAEEQKE",
    "response#2": "MSGLVLAAGEGKRMKSKLPKVLHKVCGKAMIDHVINALMGAGVEDFQVVVGHKADEVKAYLGDNIKTSYQSEQLGTGHAVMCSRDFLRNKEGTVIILAGDAPLITAETISKVFEYHNSKGYSATVLTADTENPEGFGRIVRDEHGDIRKIVEHKDASHEERLIKEINSGTYCFNINDLLNALNKLTNNNAQGEYYLTDVIEILKNEGRKVGAYKSSFTEFMGVNTRAQLYEANDFSETLGVNDRVALSQAERVMLRRINHFHMRNGAILADASRIDIRGDVAIGEDTFVDVNAVLEGRVRIGKNCQIGPNCTIINSSIGDNSVIHANTVIQDSSLGDTAEIGPFARIRPGTVTESRVKIGNFVETKKSHIDEGSKVNHLTYVGDAEIGKGVNIGAGTITCNYDGVNKHKTVIGDKAFIGSNTSLVAPLNIGEGATIGAGSTVSKDAKENALTVTRAKQTNKPGYAKIIEERK",
    "response#3": "AVIMAAGKGTRMHSKLPKVMHSLAGKPLIEHVLDVANQVGVERPFVIVGHGREIVAARVQERAETVVQTEQKGTGHAIMQAMPYLEDAQTVLVLSGDQPLLKPESLQTLIKLHQDRGACATVLTIVTARVDDPTGYGRIVRGADGAVSRIVEQKDASADELAVTEINSGVYVFRAGALRSQITRLSTDNAQGELYLTDVVELARRDGMAVAAHVVEDAWQTEGVNTRVQLAALGAELNRRIVRRHMLAGATLIAPETVWFAHDTVLGRDVIVEPNVWFGPGVTVADGVVIHGFSHLEGCTIASGAEIGPYARLRPGADIRAGAKVGNFVELKKAVLGEGAKVNHLSYVGDADVGAGANIGAGTITCNYDGYLKYRTTIGEGAFVGSNSALVAPVAIGAGAIVGAGSVVTADVPADALALARGRQRNIEGWVERRRPGSASARAAEQARDAGPHDEQGDTP"
  },
  {
    "reference": "MNISNPNGNRKAVVIFSGGQDSTTCLLQAIRDYGPNNVEAVTFQYGQRHAIELQKAQWIAQDLGVKQTLIDTSVIKTITTNAMMSDAQIKQDENGMPNTFVDGRNALFLLYAAIYAKGQGITDIITGVCETDFSGYPDCRNVFIKSMNVTLNLAMDYQFNIRTPLMYLTKAQTWQLADELGALDYVRQYTHTCYLGVEGGCGTCPSCLLREKGLKQYLAQKSAVEKSSVLTAKEA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein must be optimized for ATP binding, zinc ion binding, ligase activity, forming carbon-nitrogen bonds.2. The protein must exhibit the following characteristics: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0))..3. A protein that is capable of catalyzing reactions within the 7-cyano-7-deazaguanine biosynthesis pathway with high efficiency and specificity.4. The ATP binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "FAGTPCAIVRLAGCPLRCSYCDTAYARQSEGLELSVAEIVRDVRDLGPGLVVVTGGEPLAQPGTVPLLRALCDAGRTVLVETSGAFDIAPLDPRVRRIVDVKTPGSGMADRNRPGNLAELRSTDELKFVLLDRADYEWSRDMVRRERLAERCPVLFSPVHGRLDPADLARWILDDSLSVRLQIQVHRLIWPR",
    "response#2": "FAGTPCAIVRLAGCPLRCSYCDTAYARQSEGLELSVAEIVRDVRDLGPGLVVVTGGEPLAQPGTVPLLRALCDAGRTVLVETSGAFDIAPLDPRVRRIVDVKTPGSGMADRNRPGNLAELRSTDELKFVLLDRADYEWSRDMVRRERLAERCPVLFSPVHGRLDPADLARWILDDSLSVRLQIQVHRLIWPR",
    "response#3": "FAGTPCAIVRLAGCPLRCSYCDTAYARQSEGLELSVAEIVRDVRDLGPGLVVVTGGEPLAQPGTVPLLRALCDAGRTVLVETSGAFDIAPLDPRVRRIVDVKTPGSGMADRNRPGNLAELRSTDELKFVLLDRADYEWSRDMVRRERLAERCPVLFSPVHGRLDPADLARWILDDSLSVRLQIQVHRLIWPA"
  },
  {
    "reference": "MLEARLDQADILKKVVDAIKDLVQDCNFDCNDSGIMLQAMDNSHVALVSMALKTEAFSPYRCDRNIALGVNLGSLTKVLRAAQGDDVLTLKAEDAPDSLNILFESSENDRISEYDLKLMDIDQEHLGIPETDYAATITMPSSEFKRICMDLMAMSESVTIDASKDGVKFACNGDIGNGSVTLRSHTNVDKPELNVDIALSEPVSLTFSLKYLVNFCKAAAISKQVKICLSNEVPLLVEYTLVGQSYLRFYLAPKASLPETLVAESRLTRTSI",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must exhibit the following characteristics: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand..2. A protein that contributes to mitotic DNA replication lagging strand elongation, positive regulation of DNA replication, nucleotide-excision repair, error-free translesion synthesis, establishment of mitotic sister chromatid cohesion by functioning in chromosome, telomeric region, nucleoplasm, chromatin with DNA binding, DNA polymerase processivity factor activity, identical protein binding.3. The designed protein should contain one or more Proliferating cell nuclear antigen PCNA C-terminal, Proliferating cell nuclear antigen PCNA N-terminal domains that are essential for its function. The designed protein sequence is ",
    "response#1": "IWFACGPYTATDNCGYEHLCELLDKVVAEKPDILMLAGPFVDKKNTFLNKPTFNITYDNLLEDLLLKVKETLVGTKTQVIIQPNASRDLCVPPVFPSAPFQQNRKLDKIKKELIFVADPCIFRISGVEVAMTSSEPIQALSNTEFHRSANQENIDRVARLSSHLLTQQCMYPLEPTEVPASMGDLLDVCCIGSTPHIVFAPTKLAPSPYNIIRSLLDTAYTKNVSNIETLSRDVAAEGYSTGIILSQLHDVLLKEETLSSPVKYKIFMKLSEVDKRLNDGADETLQLLDLLS",
    "response#2": "IWFACGPYTATDNCGYEHLCELLDKVVAEKPDILMLAGPFVDKKNTFLNKPTFNITYDNLLEDLLLKVKETLVGTKTQVIIQPNASRDLCVPPVFPSAPFQQNRKLDKIKKELIFVADPCIFRISGVEVAMTSSEPIQALSNTEFHRSANQENIDRVARLSSHLLTQQCMYPLEPTEVPASMGDLLDVCCIGSTPHIVFAPTKLAPSGLPDTKYDCTVSMSSGEFQRICRDLGMLSENISIEIIKSQIKFSAKGEFGNGSVTLKQGSSSIDDNDSNTATVIDCEKPVNLTFSVKYLTHFAKAASISNTVTLSLTPDVPILVQFNIGEIGHLQYYLAPV",
    "response#3": "IPDNDYDVTIKMSSAEFSRICKDLANFGETMNISADKESVRFGVEGDVSAASVVLRPNETIDEDEKIVSTSIMVKKETNVNLSMKFLKTFANSSLAGSVTLELQDDIPCLIKYVFPNDLGVLCFYLAPP"
  },
  {
    "reference": "MKVTMALAALCVALQAPCIGSESTPSNLNNRHLRHEHDSNTPLQRRDEALVPAHRVYDPVSGLACSLVGKCMVCPTSEKDESYCRETGYRQELDCPRVEDDVVHTKSVNQRTTRFRPCSFAEPARPGVAFVKFEVGNLRTLLQKL",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein should have a specific function within the extracellular region. The designed protein sequence is ",
    "response#1": "PGDGGPPGQPGPDGPPGAPGNAGEDGKPGDQGPRGPPGIPGAPGQPGRPGPPGEPL",
    "response#2": "GRAGAPGRPGPPGQPGPAGPPGENGKGGGQGPSGLPGPPGQPGQNGAPGEVGQPGDNGL",
    "response#3": "PGDGGPPGQPGPDGPPGAPGNAGEDGKPGDQGPRGPPGIPGAPGQPGRPGPPGEPL"
  },
  {
    "reference": "MKMTFRWYGEGNDSITLKQIKQIPGCSGLMGVIDQYAAGEVWEESVIAEYVEHVHKAGLEVEVIESVNVHEDIKLGLPSRELYIENYIKTIRNLAKHGIKVIVYNFMPVLDWLRTDLARVIEEDGSNSLYYDEEELLGLTPLQIVENTSKSSNGFTLPGWEPERLKELENVLALYKNIDPDKLRENYKYFLEKVIPVCEECGVKMAVHPDDPAWPIFGLPRIAHSEELFDKILELYDSESHTICLCTGSLGSNPENNIPEIIRHFGGKNRVGCFHVRNVKYLGYRKFRESSHLSSDGSLDMFEIMKAIHETAPDTYIRPDHGRMIWDEVGRPGYGLYDRALGIAYLNGIWEALEKSSK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein must exhibit the following characteristics: Catalyzes the dehydration of D-mannonate..2. The designed protein must be able to regulate glucuronate catabolic process. The designed protein sequence is ",
    "response#1": "MSSSN",
    "response#2": "LXATXH",
    "response#3": "MVPRLLMTC"
  },
  {
    "reference": "MRVTGIRKXLWSWGILLLGILMICSAAXNLWVTVYYGVPVWREANATLFCASDAKGYKTEAHNVWATHACVPTDPNPQEVVLENVTENFNAWENGMVEQMQEDIISLWDQSLKPCVKLTPLCVTLNCTXXXXXXXXXXXXXXXXXXXXXXXNCSFNITTSLRDKWQREYAIFYKSDLVPIDNDNTTSYRLISCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNDKKFNGSGLCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEDVVLRSKNFSKNDNTIIVQLKEPVKIECTRPNNNTRKSIHIGPGRAFYATGDIIGDIRKAYCTLNGTKWKDTLKQVVSKLTEQFGNKTMVFKSSSGGDPEVVMHIFNCGGEFFYCSTTELFNSTWNGTTEZSNSTRENITITXPCRIRQIINMWQEVGKAMYAPPIAGXIKCTSNITGLLITRDGGXXXXXXEIFRPIGGNMRDNWRSELYKYKVVKIEPLGVAPTRAKRRVVQREKRAVGLGALFLGFLGTAGSTMGAASITLTVQARQLLSGIVQQQGNLLRAIEAQQHLLQLTVWGIKQLQARVLAXXRYLRDQQLLGLWGCSGKLICTTAVPWNTTWSNKSLEKIWDNMTWMQWEKEIENYTDEIYRLIEISQNQQEQNEQELLELDKWASLWNWFNITNWLWYIKIFIMIVGGLIGLRIVFTVLSIVNRVRKGYSPLSFQTHFPAPRGPDRPEGIEEEGGETDRGRSGGLVNGFLAIVWVDLRSLCLFLYHRLRDLLL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain2. The designed protein should have structural molecule activity within viral envelope, host cell endosome membrane to regulate fusion of virus membrane with host plasma membrane.3. The protein must contain a signal peptide for proper functionality.4. The designed protein have Retroviral envelope protein GP41-like domains with specific sequence motifs that are crucial for its activity.5. Prioritize Basic and acidic residues compositional bias within the protein sequence to achieve desired characteristics. The designed protein sequence is ",
    "response#1": "MRVMGTQRNCQQWWIWGILGFWMLMICNVVGNLWVTVYYGVPVWRDAETTLFCASDAKAYDKEVHNVWATHACVPTDPNPQEIALENVTENFDMWKNNMVEQMQTDIISLWDQSLKPCVQLTPLCVTLNCTDPTLNCTDPHNNRSEGISNNSNNSCMYEEMKNCTFNMTTELRDKRKTVHSLFYKLDIVPTDINNSGEYRLINCNTSTMTQACPKVTFEPIPIHYCTPAGFAILKCKDTEFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEKVMIRSKNITDNSKIIIVQLTKPVNITCIRPNNNTRTSIRIGPGQTFYATGAVTGDIRKAYCEVNRTEWNSTLQEISSQLRNYFKNKTIIFKNSTGGDLEITTHSFNCGGEFFYCNTTSLFNSTWDENSTVTNTEANGTIILPCRIKQIINMWQRVGQAMYAPPIRGRIRCESNITGLLITRDGGGGNNSTNETFRPIGGDMRDNWRSELYKYKVVKIEPIGVAPTRAKRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQARNLLSGIVQQQNNLLRAIEAQQHMLKLTVWGIKQLQARVLAVERYLHDQQLLGIWGCSGKLICTTNVPWNSSWSNKSFNEIWDNMTWLQWDREIDNYTEIIYTLIEESQNQQEKNEQDLLALDKWESLWSWFSITKWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTLIPNPRGPDRLGGIEEE",
    "response#2": "MRVKGIRKNCQHLWKWGTMLLGMLMICSAAEKLWVTVYYGVPVWRDAETTLFCASDAKAYDKEVHNVWATHACVPTDPNPQEIALENVTENFDMWKNNMVEQMQTDIISLWDQSLKPCVQLTPLCVTLNCTDPTLNCTDPHNNRSEGISNNSNNSCMYEEMKNCTFNMTTELRDKRKTVHSLFYKLDIVPTDINNSGEYRLINCNTSTMTQACPKVTFEPIPIHYCTPAGFAILKCKDTEFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEEKVMIRSKNITDNSKIIIVQLTKPVNITCIRPNNNTRTSIRIGPGQTFYATGAVTGDIRKAYCEVNRTEWNSTLQEISSQLRNYFKNKTIIFKNSTGGDLEITTHSFNCGGEFFYCNTTSLFNSTWDENSTVTNTEANGTIILPCRIKQIINMWQRVGQAMYAPPIRGRIRCESNITGLLITRDGGGGNNSTNETFRPIGGDMRDNWRSELYKYKVVKIEPIGVAPTRAKRRVVEREKRAVGLGALFLGFLGAAGSTMGAASITLTVQARRLMSGIVQQQNNLLKAIDAQQQMLQLTVWGIKQLQARVLAVESYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLNQIWDNMTWMEWEREISSYTNVIYNLIQESQNQQEKNEQELLELDKWASLWNWFSITKWLWYIKIFIMIVGGLIGLRIVFAILSIVNRVRKGYSPLSFQTRLPTQRGPDRPEGIEEEGGERDRD",
    "response#3": "MKARGMQRNYQHLWRWGTMLFWIMIICNAENLWVTVYYGVPVWKEATTTLFCASDAKAYSKEVHNVWATYACVPTDPSPQEIPLENVTENFNMGKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTEVKTNDTSTLWVTVYYGVPVWKDANTTLFCASDAKAYSTEKHNIWATHSCVPTDPNPQEILLENVTENFNMWKNGMVEQMHEDIISLWDESLKPCVMLTPLCVTLNCTEANITKANSTGGNSTNGTSSTNHSIVNEIGEVKNCSFNVTTEIIDRKQQEYALFYRLDIVPINNSTTEYRLINCNVTTIKQACPKASFEPIPIHYCAPAGFAILKCNDKEFNGTGPCKNVSTVQCTHGIKPVVSTQLLLNGSLAEGGIRIRSENFTDNTKTIIVQLNSSVEINCTRPGKTQKQIRIMHIGPGRAFHVNKEIGGMRQAHCNVSKEKWGRMLNQVATQLEEKYNRSIFFNSSSGGDIEITTHSFNCGGEFFYCNTSSLFNSSWIKRNYTETNSTNNETITLSCRIKQIVNMWQRVGRAMYAPPIPGEIRCDSNITGLLLTRDGGNKTNDGEVFRPIGGNIRDNWRSELYRYKVVAIEPLGVAPTRAKRRVVEREKRAIGLGAVFIGFLGAAGSTMGAASLTLTVQARRLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQLMGIWGCSGKLICTTAVPWNTSWSNKTLNQIWDNMTWMEWEREINNYTGLIYNLIEESQNQQEKNEQELLELDKWASLWTWFDISNWLWYIRIFIIIVGGLIGLRIVFAVLSIVNRVRQGYSPLSL"
  },
  {
    "reference": "MGEKAILDLHLVSDSTCETVVSVARAAVEHFKSLEVNEFVWSLVGSKSHVDRIISSIDRKRNNLIMYTVLDDDLREYLKKKAEAYNIRCIPILSHIIREISCYLSVAKDPNAHPHRLSDEYFNRIEAINYTIAHDDGQSLWDIDGADIIIVGVSRTSKSPTSIYLAYRGYKVVNIPFVHSITLPIDSAALADKLVVGLTIDIDRLIQIRRNRLISMKNQSNCNYISYEQVEQEISEVNRICMKNQWPLIDVTQKSIEETAATIIQFFNRKNNKIGGDAFC",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The designed protein should be able to compete with endogenous proteins or inhibitors for ADP binding.2. The designed protein should be optimized for phosphotransferase activity, phosphate group as acceptor activity in order to improve protein dephosphorylation, protein phosphorylation. The designed protein sequence is ",
    "response#1": "IHACNSQGHWGAGIAKAFKQKYPKAYTAHNKFCAKEHSKTQPVPTGSAQLLAPVDSGSQHWIGCLFTSAKYGKAKDKPDAIVKNTTASMSMLLELVRMADGEVSEIRMCKINSGKFGVPWEKTEEC",
    "response#2": "PMESTLLSKSLEAAQKKVEAYFYDTRKQVFEYDQVLNSQRQAIYAERRRILESGYPRDCILQYAESTIDDIVNFCLTSKENNEKFVNLNTKIKYR",
    "response#3": "MIASIGELLIDLISVEEGDLKDVRLFEKHPGGAPANVAVGVSRLGVKSSLISKVGNDPFGEYLIEELSKENVDTRGIVKDEKKHTGIVFVQLKGASPSFLLYDDVAYFNMTLNDINWDIVEEAKIVNFGSVILARNPSRETVMKVIKKIKGSSLIAFDVNLRLDLWRGQEEEMIKVLEESIKLADIVKASEEEVLYLENQGVEVKGSMLTAITLGPKGCRLIKNETVVDVPSYNVNPLDTTGAGDAFMAALLVGILKLKGLDLLKLGKFANLVAALSTQKRGAE"
  },
  {
    "reference": "RRTKRFASLSRFVETLVVADDKMAAFHGAGLKHYLLTVMAAAAKAFKHPSIRNPVNLVVTRLVILGSGQEVPQVGPSAAQTLRSFCTWQKGLNPPNDSDPDHFDTAILFTRQDLCGVSTCDALGMAGVGTVCDPARSCAIVEDDGLQSAFTAAHELGHVFNMLHDNSKPCANLNGQGSSSRHVMAPVMAHVDPEEPWSPCSARFITDFLDNGYGHCLLDKPEAPLHLPVTFPGKDYDADRQCQLTFGPDSSHCPQLPPPCAALWCFGHLNGHAMCQTKHSPWADGTPCGPAQACMGGRCLHVDQLKDFNIPQAGGWGPWGPWGDCSRTCGGGVQFSSRDCTKPVPRNGGKYCEGRRTPFRSCNTKNCPHGSALTFREEQCAAYNHRTDLFKSFPGPMDWVPRYTGVAPRDQCKLTCQARALGYYYVLEPRVADGTPCSPDSSSVCVQGRCIHAGCDRIIGSKKKFDKCMVCGGNGSSCSKQSGSFKKFRYGYSDVVTIPAGRTHILVRQQGGSGLKSIYLALKLADGSYALNGEYTLMPSSTDVVLPGAVSLRYSGRTAASETLSGHGPLAQPLTLQVLVAGNPQNVRLRYSFFVPRPVPSTPRPPPQNWLQRRAEILEILRKRTWAGRK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The protein must be engineered to have a high affinity for Zn(2+) in order to increase its catalytic activity.2. The protein should have metalloendopeptidase activity and be active in extracellular space. The designed protein sequence is ",
    "response#1": "FLESENREVRKNAFDAVYKTYGQYKNTMATTLSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLVKTINKHLPLLHRYIALRKKVLELDEVHIYDLYTPLVKDAGMKVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRNGAYSSGAYGTNPYILMNWHNNVNNLFTLVHEFGHSVHSYYTRKHQPYPYGNYSIFVAEVASTTNEALLGEYLLNNLEDEKQRLYILNHMLEGFRGTVFRQTMFAEFEHLIHTKAQEGEPLTPELLTNVYYDLNKKYFGDGMVIDKEIGLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVDRYIDFLKAGSSQYPIDVLKKAGVDMTSPEPIEAACR",
    "response#2": "SGLVYRDMSGGINEAFSDIAGEAAEYFMRGNVDWIVGADIFKSSGGLRYFDQPSRDGRSIDHASQYYSGIDVHSSGVFNRAFYLLANKSGWNVRKGFEVFAVANQLYWTPNSTFDQGGCGVVKAAQDLNYNTADVVAAFNTVGR",
    "response#3": "FLESENREVRKNAFDAVYKTYGQYKNTMATTLSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLVKTINKHLPLLHRYIALRKKVLELDEVHIYDLYTPLVKDAGMKVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRNGAYSSGAYGTNPYILMNWHNNVNNLFTLVHEFGHSVHSYYTRKHQPYPYGNYSIFVAEVASTTNEALLGEYLLNNLEDEKQRLYILNHMLEGFRGTVFRQTMFAEFEHLIHTKAQEGEPLTPELLTNVYYDLNKKYFGDGMVIDKEIGLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVDRYIDFLKAGSSQYPIDVLKKAGVDMTSPEPIEAACE"
  },
  {
    "reference": "MSDNSGSGTSPWGSLNTPVGPPKVTLAYFSNEFPPDDLNFIVRKLFDRTSKGPFCSIDGVLLCAIQFANLIGHYETTDHLFPFGSSIASVAGLGIGLVAAAAVSVTPSLADLPVAGAEAVRIAFRLGVLVDGVSQNLQPRDRSTTGTPDSWAYVIPDVSPEVVQKELDEIHSREKTPIPSKIFVSALSRTSVTISGPPARLRSLFRLSDFFRDRKFVALPVYGGLCHAGHIYEQRHVQEVVEKSVLDETHVRYSPSVRLFSTSTGKPFLSTSVTNLFEQVVGEILTQKIQWDKVVKGVLERIQELSATEVEVLVFRDSLPVHELVKALKSADSGLQTTTEDLLQWLHQSRERLQGPRGSLQSKIAIVGMSCRMPSGATDTEKFWELLEKGLDVHRKIPADRFDVETHHDPTGKRVNTSITPYGCFIDEPGLFDAGFFNMSPREAQQTDPMQRLALVTAYEALERAGYVANRTSATNLHRIGTFYGQASDDYREVNTAQEISTYFIPGGCRAFGPGRINYFFKFSGPSYSIDTACSSSLATIQAACTSLWNGDTDTVVAGGMNVLTNSDAFAGLGNGHFLSKTPNACKTWDCEADGYCRADGIGSIVMKRLEDAEADNDNILGVILGAGTNHSADAISITHPHAPSQAFLYRQILRDAALDPFDVSFVEMHGTGTQAGDSEEMQSVTEVFAPIANKRRTSKQPLHIGAVKSNVGHGEAVAGVTALIKVLLMFQKEAIPPHAGIKNSINPGFPKDLDKRNINIPYQKTAW",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should contain one or more Ketosynthase family 3 (KS3) domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MDPQQRLLLEVVFEAFEDAGISLEEMNGSRTSVLCGAFTNDYNAMLTKDLEYYPKYTVTGTGNAILANRISYAFNLKGMSLTIDTACSSSLVGFHLGAQAILNGDCEMAIIVGSALHFDPNIFITMTDLGMLSQEGRCRAFDAGGKGYARGEGICAVILRGQMQAEMHGDHIRALVRATGSNHDGMTQGITLPSSEAQEALIRRTYQSCGLDPADTQYVEAHGTGTARGDPLEMRAIGACFSSPRRSDPLYVGSVKSNIGHAEGASGLAGLIKASMALEKGQIPPNMHFKHPNPEIAFADWQIEVPTRVIDFPSNAQGTRRVSINSFGYGGSNAHVILESYDQ",
    "response#2": "VPIPQYLGDNGAMIAYTGKIMFESGSTLPVELSRVNPSFRADQVEVTWRDEPVVSGNHEESATTRGAEAVVSFTDITATKTRVSKRYRHPELDKRLINERTRAEARLISEARRVGVRTPIIREITHDAIVMERIIGQKLKENLTPETLIETGRMIGRLHSSQIIHGDLTTCNFLVQGNKLWLIDFGLALTSSEIEHRGVDIHVLYQILESTSNDSDNLKKSFMKGYRELMPEADEILTREHEIELRGRYR",
    "response#3": "MDPQQRLLLEVVFEAFEDAGISLEEMNGSRTSVLCGAFTNDYNAMLTKDLEYYPKYTVTGTGNAILANRISYAFNLKGMSLTIDTACSSSLVGFHLGAQAILNGDCEMAIIVGSALHFDPNIFITMTDLGMLSQEGRCRAFDAGGKGYARGEGICAVILRGQMQAEMHGDHIRALVRATGSNHDGMTQGITLPSSEAQEALIRRTYQSCGLDPADTQYVEAHGTGTARGDPLEMRAIGACFSSPRRSDPLYVGSVKSNIGHAEGASGLAGLIKASMALEKGQIPPNMHFKHPNPEIAFADWQIEVPTRVIDFPSNAQGTRRVSINSFGYGGSNAHVILEL"
  },
  {
    "reference": "MKLQTLLSQLQNIKQNVNEDPDILSIEMDSREVKEGSLFICISGYTVDGHDYAEQAVRLGAAAVISEKPLSLSVPVIVVKNSKRAMSMLADSFYGQPTQKLHLIGVTGTNGKTSTTHLIEKILQNAGKTTGLIGTMYIKIGEEQFNVKNTTPESLTLQKTFNAMVEKGVNSAIMEVSSHALHMGRVHGCDYDVAVFTNLTQDHLDYHQTMDSYRAAKGLLFAQLGNKFDHNKPKYAVLNGDDPASAEFMNMTAAHVFTYGIDSECDIMAKNILMSSSGTEFDLVTPFGTEKVKMKLIGKFSIYNALAAAAASLASDVPTSVIAATLQSIEGVRGRFEVVDGGQDFSVIVDYAHTPDSLENVLTTVKQFVKGKTFVVVGCGGDRDRTKRKVMAQIAVKYADEPIFTSDNPRSEDPMDILNEMEDGVRGEYYHSIVNREQAIYFAVANAKAGDVILIAGKGHETYQQIGTQTFDFDDREVALKAINELVK",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.2. The presence of Mur ligase central, Mur ligase N-terminal catalytic domains is critical for the interaction of the designed protein with its intended targets.3. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.4. The designed protein should be optimized for ATP binding, tetrahydrofolylpolyglutamate synthase activity, magnesium ion binding activity in order to improve regulation of cell shape, cell division, peptidoglycan biosynthetic process.5. The UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MTLDSRVAAAGDLFVAVKGHQADGRRYIPQAIAQGVAAVVADADSAAGHGTLTMTHGVPVIAIHNLQQQLSALAGRFYGEPSRALRLVGITGTNGKTTTSYLLEAILKEAGAEVGVIGTVNYRWGNRMLPALNTTPSPCELQRILREMASHGVTHVVMEVSSHALVQRRVKACHLDVGIFTNLSPEHLDYHGTMEEYLAAKALLFNEVLKESAKEPWAIYNRDDRWVRKAAEEVNGLKKMDYGTEGTIRPLRWRTDLEGIEAVIDTPRGILEVSSSLIGPFNLENIMAAVASVFALGFDFEQLVTALPKLQPPAGRLQLAGRHPAGARVYVDYAHTPDALGNVLTALRPHTRGKLVVVFGCGGDRDRGKRPLMGQVAAELADRVFVTDDNPRSEVPALIRQEILASCPTAHEIGDRREAIRAAVASLAADDVLVVAGKGHEDYQILGTEKIHFDDREQAREALAARTP",
    "response#2": "MTLDSRVAAAGDLFVAVKGHQADGRRYIPQAIAQGVAAVVADADSAAGHGTLTMTHGVPVIAIHNLQQQLSALAGRFYGHPSRSLQLVGVTGTNGKTTTTYLIDAIYTHAGIRTGTLGTTGLKVGDSLQALPNTTPESLTLQQAFRDMVDQQVTAGIMEVSSHALVTGRTRGCQFDVAIFTNLTQDHLDYHQTLTAYQDAKRLLFASLPAKTKTGKPTFAILNVDDKASAGFRDVTQGLVVTYGLENEADFSASQLVLKSKGTTFNLKTPDGFFAMTLPLAGKFNVYNALAAAAAALATGVDVAEVERGLSAVAGVPGRLQLAGRHPAGARVYVDYAHTPDALGNVLTALRPHTRGKLVVVFGCGGDRDRGKRPLMGQVAAELADRVFVTDDNPRSEVPALIRQEILASCPTAHEIGDRREAIRAAVASLAADDVLVVAGKGHEQGQIVGDRTLPFSDVEVAREAA",
    "response#3": "MKLAELLSKLHPIHLSGDLDIDINAIQFDSRQVQNNDVFVAQNGVTVDGHQYIEKAINQGAKTVVCEVMPEEKNEQVTYIKVEDSNVALAMLAANYYEHPSKDLKLVGVTGTNGKTTTTYLIDAIYTHAGIRTGTLGTTGLKVGDSLQALPNTTPESLTLQQAFRDMVDQQVTAGIMEVSSHALVTGRTRGCQFDVAIFTNLTQDHLDYHQTLTAYQDAKRLLFASLPAKTKTGKPTFAILNVDDKASAGFRDVTQGLVVTYGLENEADFSASQLVLKSKGTTFNLKTPDGFFAMTLPLAGKFNVYNALAAAAAAYAEGLEIETIIKALENVKGVAGRMEVFHAAGKPTVVVDYAHTPDALEKALAAARLHCHGRLWCVFGCGGDRDKGKRPLMGGIAEQYADRVVITDDNPRSEAPMSIINDILAGLLNAGAAAVIPGRAEAVTSAVMQAGEQDVVLVAGKGHEDYQILGDKTIHFDDREVAREALEKKGVL"
  },
  {
    "reference": "MKLQIPAFDNARVLIAGDVMLDRYWHGDTSRISPEAPVPVVRVDAIEERPGGAGNVALNIAALGGQARLMGLTGDDEAADSLQRLLEAAGVDCAFERMSGHPTVTKLRVLSRHQQLIRLDFEEGFSGVDHAALTDRFEALLADTDVVILSDYGKGALRHAREFIEAARAAGKPVLVDPKSSDFSHYRGATLITPNLKEFEAVVGPCADEDEIVEKGMALIERERLGGLLVTRSEQGMTLLMAGKPPFHQPTRAREVFDVTGAGDTVISTLAAGLAAGLEMRQAVLLANMAAGIVVGKLGTATVTVPEIRRALHEHDDSLQGVLSEEQLMLAVEDARAHGERIVFTNGCFDILHEGHVAYLEQARRLGDRLIVAVNDDDSVRRLKGETRPINPLDRRMAVLAGLAAVDWVVPFSEDTPERLICRVRPDLLVKGGDYRPEDIAGHDCVVGAGGEVRILDFLDGCSTTDIVERIRNSERG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must have high specificity towards ATP binding. The designed protein sequence is ",
    "response#1": "RALPDIRDGLKPSQRRVLYAMKQLSLSPGAKHRKCAKICGDTSGDYHPHGESVIYPTLVRMAQNWAMRYPLVDGQGNFGSIDGDPPAAMRYTEARLTHSAMYLMEDLDKDTVDIVPNYDETKHEPVVFPSKFPNLLCNGSSGIAVGMATNIPPHNLGELIEATLLLLANPQASVDEILQVMPGPDFPTGGIICGSEGIRSTYTTGRGKIKVRARLHVEENEDKHRESIIITEMPYNVNKSRLIEQIANLVNEKTLAGISDVRDESDKDGIRVVLEIKKGESSEIIINRLYKFTDVQVTFGANMLALDKNLPRTMSIHRMISAWIRHRKEVIRRRTRYELNKAETRAHVLEGYLKALSCLDALVKTIRESGNKEHAKERIIESFGFTEPQALAILELRLYQLTGLEAEKIQKEYEELLNKIAYYKQVLSDEGLVKDIIRNELE",
    "response#2": "QLVIIPVSQHKEGVLEKANELKQKLSAKFRVKMDDSDKMPGWKFSEYEMKGVPLRIEIGPKDIEKNQAVLVRRDNREKIFVSLDNLEETVVY",
    "response#3": "QQLLNVGFDYYNLPSSDGSHYWSDNVAYEFTLAEIDRIEDTTNELHSMCLDFAADEIKKGDYENYHFTELQKQLIETSWRNQEPYLYGRFDFGYDGNNLKMFEYNADTPTSLLEAAVVQWQWLEQIEGLKHRDQFNWIHEELIKHFQFLKQQSGKTDFHLSAMQDAGREDWGNVDYLADVAYNAGWNIHQLAVEDIGYNSETKKFVDLNDQPIEMLFKLYPLEWLSHAEFARHITTAETRFIEPAWKMLLSNKALLAKLWARYPNHPHLLPAYFTPFELPKDLSMWVKKPLLGREGANVFYYEKNNGVEFAAKGSEHSTFYGNSGYVYQQKFELPSFDGMYPIIGSWVVGDVACGMGLREDFTAVTGNDSHFIPHYR"
  },
  {
    "reference": "MRGYGRAGSAVTVVNAFATGKGGAVGIDLWTEAKVKLVEDGISGSITVRGESFGDTRLVRAVVEVVRRATGEDFGIEFEISSEIPVGKGLKSSSAAANALVLAIYEALGIEIAPLEAIKLGVEAARLAGVTITGAFDDASASLLGGLCLTDNTRDELLKREEVEPEPVVLLIPEESIMTSSLKGMDFSGIAPYITEAFDMAMRGEWRKALVINGLVYSAFLGHPTEPIGTALRLGAIAGLSGKGPAFFALTREPEVLSEEWSRFGKTEITKLR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should be able to bind ATP ligand in a variety of conditions2. A protein should be able to manipulate the activity of enzymes in the Metabolic intermediate biosynthesis, chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7 pathway, either by activating or inhibiting their function.3. The designed protein should be optimized for ATP binding activity in order to improve amino acid biosynthetic process. The designed protein sequence is ",
    "response#1": "VIEVIGELISKPYIEITLNLLARFGIAVRREGWQRFTIPAGSAYVTPGEVHVEADASSASYFIALGALAGVDAPVRIEGVGAASIQGDIRFVEAAQAMGARVMAEPNALEVRRGAWPLKAIELDCNHIPDAAMTLAVMALYADGPSTLTNIASWRVKETDRIAAMATELRKLGAQVEEGADHIRVWPLAQFKPAAIHTYDDHRMAMCLSLAAFHTLKGAAPVPVRILDPKCVAKTYPDYFETL",
    "response#2": "AGTVRLPGSKSISNRLLLLAGLSAGVTTLVDLLDSDDTRVMLDALRGLGCRIEQQGRHTRITGVAGKPAVLEADFFLGNAGTAMRPLTAALAVISTQNGGRYTLSGVPRMHERPIGDLVDALRALGVAVECLGNEGYPPLRLAGGSLLLDAPVRVRGDVSSQFLTALLLALPLALFR",
    "response#3": "GSVQLPGSKSISNRVLLLSALSEGTTEVRDLLASDDTRVMLDALRQIGCDVQEGLGDDAGTVRITGLGTKAPTSPASLFLGNAGTAMRPLTAALSLLGGEFELKGVARMHERPIGDLIDALRQLGCRIDYLGNEGFPPLRIQHGDGLPPLKLNESIKVRGDVSSQFLTALLMALPLVAKTQDIVIDVVGELISKPYIHITLELLARFGIIVQHDNWQRFTIAAGSRYVSPGVLHVEADASSASYFIALGAIAPSSEGQDGIQVLGVGQDSIQGDIRFVEAARAMGADIESGPNWLRIRRGAWPLKAVNMDCNHIPDAAMTLAVMALFADGTTTLTNIASWRVKETDRIAAMAIESRKLGAVIEEGPDYLRITPPATAADWKAASIHTYDDHRVAMCFSLATFNPASVPVRIEDPKCVGKTFPDYFEALFSVV"
  },
  {
    "reference": "METLKADLSDMSDSPEYFETLANRDLPRLPGTSKLHRAACIKRKSINLSLPSNSYSLSYRQSDDTDGDVSESQSEYRLSSGRRSRASFARALQDPQTPNTPPVSSQHRRFFSEGSFNLPNSNMSHSLNGDSTASNSSTLTPNRIYGDRNRQDYAQSSRYTLPSLPSSPSYNCPTTLRKIHTNTSSNGTSRRVSGLGSFMAQNSSETSSNRTSAYLPGSSTDEQEKRSSVTLASMPSSHSSTASLLSPLDTTSFSTKLDSTILALETDESLSRTISYATTSLPSTPGKRLSKESLAMSEASSINPEYKRIKKRANLIKELVTTEAAYLNDLIAIQQSYGLRVKECSALNPVDAQTVFGDIESLLTFTVEFHSRLYQAGEGSWRVNLDTQLIDPLPCNLGLIFLESLSEIGQIYTGYCNRQDSVFKIITKWREKPATASWIMEGDKIVQKYTNAWDLGSLIIKPLQRLLKYPLLLQKIIDVTPESSSERPDLVLSYQLLQELISGINQKQKPSHKRGSLSASHKRDAAWSLLYKATSNKSRPTTTSTELKTDARLNFQRQVLQDFRQRFAILKALHATLETWYVTVHRGFSVFEKVLAELEGLSALEPEDKPVDTWRKYHLLAHMMTANLPSQIQTSLNSSILNPITNILRVIQKVIQFIISAEFVIPAQKLEAISTLVEKEFHSVVYHFIGIQRSLYENYAQGFLFLIPQDMRDSILEETQDYAELVRAFEPMHYDDEVLLEELMKSVSLAARV",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that contributes to regulation of establishment or maintenance of bipolar cell polarity regulating cell shape, positive regulation of mitotic actomyosin contractile ring contraction, establishment or maintenance of bipolar cell polarity, positive regulation of establishment of cell polarity regulating cell shape by functioning in medial cortex, cell cortex of growing cell tip, cytosol, cell cortex of cell tip with guanyl-nucleotide exchange factor activity.2. The protein must exhibit the following characteristics: Involved in bipolar growth, via modulation of cdc42-shk1-orb6 signaling, and septum formation; Stimulates guanine nucleotide exchange of cdc42.; Has a role in the control of cell polarity and cytokinesis.3. Target a Polar residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "ERRSSISIYILCRVLTEIAETIPSNALEESTVSCLLECVFHQLLSAKNLPVSSSYFSLANWESFAFLVGSMSRFNFVMVSDRFIEEIEHLEKSGCDSRQKETVLVHLLRAMRYLRLQLYPTTLLEESIAFLQSLTSFFMKANTALKIEYAYLMEQLLRPLISRATFEVNIPAWSRTIETIYPVVLKMCTKTKYWNVFFPFCCTLLCLSPKQFFLKHWISSLDAAFFRVKDRRLRNTGLPHLSRVVWTYSNQYKEEPSIMNSNISNILKSGFSIGKKFSVVPFATLEELDGYAQIVRVVGAHFPELVIKEILTPLSTDAFTENIGPEKLMIVVRSVYYILHDMKYKKSC",
    "response#2": "VPVEDVCEREGALIPNIIVKLLDEIELRGLDEVGLYRVPGSVGSINALKNAFDDEGAVHNTFTLEDDRWFEINTIAGCFKLYLRELPESLFTNEKVDEFVNIMTAYKNHEVDLSQFQNGIKTLLSTLPVFNYHILKRLFLHLNRVHQHVENNRMDASNLAIVFSMSFINQDDLASTMGPTLGLLQMLLQHLIRNPEHYDAVEILEGRSFKLKYPWVGVVNRSQADINKNVDMIAARKREREYFSNTTEYRHLANKMGSEHLAKMLSKHLERVIKSRIPGIQSLINKTVLELETELSRLGKPIAADAGGKLYSIMEICRLFDQIFKEHLDGVRAGGEKVYNVFDNQLPAALKRLQFDKQLAMDNIRKLVTEADGYQPHLIAPEQGYRRLIESSIVSIRGPAEASVDTVHAILKDLVHKSVNETVELKQYPALRVEVTNAAIESLDKMREGSKKATLQLVDMECSYLTNDSYLRRIGSNVLSYVNMVCAGLRNSIPKSIVYCQVREAKRSLLDHFFAELGTMDMKRLSSLLNEDPAIMERRSAISKRLELYRAAQSEIDAL",
    "response#3": "FSTIKVIGKGAFGEVRLVQKLDTGKIYAMKSLLKTEMFKRDQLAHVKAERDLLVESDSPWVVSLYYAFQDSLYLYLIMEFLPGGDLMTMLINYDTFSEDVTRFYMAECVLAIADVHRMGYIHRDIKPDNILIDRDGHIKLSDFGLSTGFYKQDQSASYMKPRTGNTVKRGQMVDAIWLTMSSKDKMATWKKNRRVMAYSTVGTPDYIAPEIFLQQGYGQDCDWWSLGAIMFECLIGWPPFCSENSHETYRKIINWRETLTFPNDIHLSIEARDLMDRLMTDSEHRLGRGGAIEIMQHPFFTGIDWDHIRETAAPFIPNLKSITDTHYFPVDELEQVPEQPVTQQPASVDPQTLEQTNLAFLGYTYKKFNYLTMKK"
  },
  {
    "reference": "MGVTKKPDLNDPVLRAKLAKGMGHNYYGEPAWPNDLLYIFPVVILGTIACNVGLAVLEPSMIGEPADPFATPLEILPEWYFFPVFQILRTVPNKLLGVLLMVSVPAGLLTVPFLENVNKFQNPFRRPVATTVFLIGTAAALWLGIGATLPIDKSLTLGLF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. For general function, the protein need meet that Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.2. The protein can be targeted to chloroplast, thylakoid and perform electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity.3. The designed protein should have at least Helical transmembrane domains to ensure proper localization. The designed protein sequence is ",
    "response#1": "PSNPFATPLEILPEWYLYPVFQILRTVPSKLLGVLIQTTIPLGLMLIPFIENVNKFQNPFRRPIATSVFLFSVVFTLWLGIGATLPIDKSLTLGLI",
    "response#2": "LKEKLKKGMGHNYYGEPAWPNDLLYIFPVVIMGTISLVIGLAVLDPAMIGEPSNPFATPLEILPEWYLYPVFQILRTVPSKLLGVLIQTTIPLGLMLIPFIENVNKFQNPFRRPIATSVFLFSVVFTLWLGIGATLPIDKSLTLGLN",
    "response#3": "LKEKLKKGMGHNYYGEPAWPNDLLYIFPVVIMGTISLVIGLAVLDPAMIGEPSNPFATPLEILPEWYLYPVFQILRTVPSKLLGVLIQTTIPLGLMLIPFIENVNKFQNPFRRPIATSVFLFSVVFTLWLGIGATLPIDKSLTLGLM"
  },
  {
    "reference": "MRRSFLKTIGLGVIALFLGLLNPLSAASYPPIKNTKVGLALSSHPLASEIGQKVLEEGGNAIDAAVAIGFALAVVHPAAGNIGGGGFAVIHLANGENVALDFREKAPLKATKNMFLDKQGNVVPKLSEDGYLAAGVPGTVAGMEAMLKKYGTKKLSQLIDPAIKLAENGYAISQRQAETLKEARERFLKYSSSKKYFFKKGHLDYQEGDLFVQKDLAKTLNQIKTLGAKGFYQGQVAELIEKDMKKNGGIITKEDLASYNVKWRKPVVGSYRGYKIISMSPPSSGGTHLIQILNVMENADLSALGYGASKNIHIAAEAMRQAYADRSVYMGDADFVSVPVDKLINKAYAKKIFDTIQPDTVTPSSQIKPGMGQLHEGSNTTHYSVADRWGNAVSVTYTINASYGSAASIDGAGFLLNNEMDDFSIKPGNPNLYGLVGGDANAIEANKRPLSSMSPTIVLKNNKVFLVVGSPGGSRIITTVLQVISNVIDYNMNISEAVSAPRFHMQWLPDELRIEKFGMPADVKDNLTKMGYQIVTKPVMGDVNAIQVLPKTKGSVFYGSTDPRKEF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be optimized for leukotriene C4 gamma-glutamyl transferase activity, hypoglycin A gamma-glutamyl transpeptidase activity, glutathione hydrolase activity activity in order to improve glutathione biosynthetic process, negative regulation of cell cycle G1/S phase transition, negative regulation of T cell proliferation. The designed protein sequence is ",
    "response#1": "TSHVSVLGEDGSAVAATSTINTPFL",
    "response#2": "LVSLL",
    "response#3": "TSHVSVLGEDGSAVAATSTINTPFLG"
  },
  {
    "reference": "MGNQEYHEPVLADATTSLLVTRPGIYIDGTLGGGGHSLELLRRLDALDGESLLVGIDQDSYALEAAGQKLQDFGDKAILLRGNFSMVRELLAPVKRGPGEGLEVMGLLLDLGVSSFQIDTPVRGFSYLRDGPLDMRMDPDGPLTAADIVNDYEEQALARLFFRYGEEAHGGRIARAVVSARSVAPITTTGGLAEVVRRACPRKDSPIKTLSRIYQALRIEVNDELGVLQQALEDGFAVLSPGGRFAVISYHSLEDRIVKRFFSARCTADWGPKGLPLREPLKPAEAELVTRKSVQAGEAEVSRNPRARSARLRVIQKL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein must exhibit the following characteristics: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA..2. The protein should have a fast turnover rate for cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = H(+) +   N(4)-methylcytidine(1402) in 16S rRNA + S-adenosyl-L-homocysteine.3. The designed protein should have a high specificity for S-adenosyl-L-methionine binding, with minimal off-target effects.4. The protein design must be able to perform rRNA (cytosine-N4-)-methyltransferase activity in order to contribute to rRNA base methylation. The designed protein sequence is ",
    "response#1": "MAWGKKGRRGGDATKMDAERA",
    "response#2": "MNNEILDFEKVVIDEKDISSYLDGHKEDMIYLCGNSLGLQPKRTARYLEEELDTWARKGVEAHFDGKHPWMHYHEFLTEPMAKLVGAKPVEVVVMNTLTVNLHLLMVSFYRPTKERYKILIESDAFPSDQYAVESQIRFHGFDPKEAMVKLKPRQGEHLLRTEDILNAIEEHGDEIALVLLGGVNYYTGQLFDMETITKAGHEHGCIVGFDCAHGAGNVELHLHDWGVDFAAWCSYKYMNSGPGSIAGAFVHERHAHDINRPRFAGWWGHNKERRFNMQPEFEPMPTADAWQLSNPPILSMAAHKASLDIFEEAGMENLRKKSELLTGYLEFLIKEINGNENFEIITPRDPNKRGCQLSLLVHEKGKELFNYLMENGVITDWREPNVIRLAPAPFYCSFQDMYNFGQILKEGIIKK",
    "response#3": "MAGGSRRHQARKRFGQNFLVDHGA"
  },
  {
    "reference": "MTDPHLAIVVLAAGQGTRMKSQTPKLLHEIAGVPLIGHVLATAGEMDAAYTVSVVRHERDRVVEVIEDHFPSSIIADQDEVAGTGRAVEQAIAALPADFEGDVLVVSGDVPLLDADTLRGLVAEHRASAASATLLSAVLDDATGYGRIVRGDGGLLDRIVEHKDANDSELAIREINAGVYVFGLAELRDQLAQVSTDNAQGEKYLTDVIGLLRRAGSDVAAVPVSEPWLVAGINDRAQLSEAAAKLNALIVRGWQLNGVTVVDPATTWIDLKASFETDVTLLPGTQIKGATSIARGAVVGPDTTLVDCEVGEGAEVKRTDATLSVIGAGASVGPFAFLRPNTVLDADGKIGTFVETKNSHIGAGSKVPHLSYIGDTEVGVGSNVGAGTITANYDGVNKHRTRVGSHVRTGSHNVFVAPVRIGDGAYTGAGTVVRKDVPAGSLAINVAPQRNLAGWVEQNRPGTDAATAAAEAQSAEQIGE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must have at least one MobA-like NTP transferase domain.2. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity.3. The protein should have a specific function in UDP-N-acetylglucosamine biosynthetic process, cell morphogenesis and should be localized to cytoplasm in order to carry out this function efficiently.4. The protein design should consider the endogenous proteins and enzymes present in the UDP-N-acetyl-alpha-D- glucosamine biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "AVIMAGGLGTRMRSATPKHFHPLLGRRMVDWVVGAAAELAPEPLVVVTSPENLGAYDGVAVAVQKRALGTGDAVRSAAGLLEGFDGDVLILNGDVPALRPDVLRSLVEVHRDEQAAATVLSFEPKQARHYGRILRDGDRLLRIVEAVDASDEELVVPEVNSGMYVFRADALWPALERLEGANGDFYLTDVVAMARERGVAATVVEGEEAEVLGVNDRVQLAELERRLQARRLARLMMEGVTVVDPATTWIDLTASFEPDVELLPGTQIKGATTVASGAKIGPDTTLVDCEVGENAVVKRTDATLSVIGAGASVGPFAYLRPNTVLDADGKIGTFVETKNSHIGPGSKVPHLSYIGDTEVGTGSNVGAGTITANYDGVNKHRTEVGSHVRTGSHNVFVAPVRIGDGAYTGAGTVVRKDVPAGALAINVAPQKNIENWVREWREG",
    "response#2": "VILAAGMGTRMNSDLPKVLHKVAGAPLLVHAMKSGAALDPKKLVIVAGHGADEVAKAARDWDDRAEVVLQEEQLGTGHAVLQARAALEGFDGDVIVLYGDTPFIQPETIENIAAARARNDVVVLGFLTARLADPSGYGRILRDNDGNVLRIVEQKDCTPDELAITECNSAIYVFDAAVLRRALPRLGNANAQGEYYLTDALAVRAAMENGATLIAPETVFFSYDTVLGADVLVEPHVVFGPGVTVADHVVIHAFSHLEGATLAEKATIGPYARLRPGANLARGAKVGNFVEIKKAEIGEDAKVNHLTYIGDATIGPRANIGAGVITCNYDGFLKYRTEIGADAFVGSNCSLVAPVKIGDGALVGSGSVVTKDVAADALAVARGKQVNRPEWADRFRALKAARKAA",
    "response#3": "VILAAGMGTRMNSDLPKVLHKVAGAPLLVHAMKSGAALDPKKLVIVAGHGADEVAKAARDWDDRAEVVLQEEQLGTGHAVLQARAALEGFDGDVIVLYGDTPFIQPETIENIAAARARNDVVVLGFLGFRPADPGRYGRLIVEGDRLLRIVEFKDATEDERAVTLCNSGLMAVRGHDVFKLLERVGNANAKGEYYLTDVVGIARADGRACAVVEGDETEVLGVNSRGELAQIEAILQQTRRLEALA"
  },
  {
    "reference": "MKAKVGDWLVIKGATIDQPDHRGLIIEVRSSDGSPPYVVRWLETDHVATVIPGPDAVVVTAEEQNAADERAQHRFGAVQSAILHARGT",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be designed to localize specifically to cytosol, peptidoglycan-based cell wall in order to perform its role in response to hypoxia more effectively. The designed protein sequence is ",
    "response#1": "SMSPSDAASIQAIKRVGQVFGEFDSDNAVTIVLEGDQPLGGDAHRFYSDLMRKLSADTRHVAHIQDFWGDPLTAAGSQSADDRAAYVVVYLVGNNETEAYDSVHAVRHMVDTTPPPHGVKAYVTGPAALNADQAEAGDKSIAKVTAITSMVIAAMLLVIYRSVITAVLVLIMVGIDLGAIRGFIALLADHNIFSLSTFATNLLVLMAIAASTDYAIFMLGRYHESRYAGEDRETAFYTMFHGTAHVILGSGLTIAGAMYCLSFARLPYFETLGAPIAIGMLVAVLAALTLGPAVLTVGSFFKLFDPKRRMNTRRWRRVGTAIVRWPGPR",
    "response#2": "SMSPSDAASIQAIKRVGQVFGEFDSDNAVTIVLEGDQPLGGDAHRFYSDLMRKLSADTRHVAHIQDFWGDPLTAAGSQSADDRAAYVVVYLVGNNETEAYDSVHAVRHMVDTTPPPHGVKAYVTGPAALNADQAEAGDKSIAKVTAITSMVIAAMLLVIYRSVITAVLVLIMVGIDLGAIRGFIALLADHNIFSLSTFATNLLVLMAIAASTDYAIFMLGRYHESRYAGEDRETAFYTMFHGTAHVILGSGLTIAGAMYCLSFARLPYFETLGAPIAIGMLVAVLAALTLGPAVLTVGSFFKLFDPKRRMNTRRWRRVGTAIVRWPGPQ",
    "response#3": "LSEKLEYLVKDMDILITLLPQMRAQMPPMISAMTTMRDMMLIWHGTLGAFYKQQERNNKDPGAMGRVFDAAQIDDSFYLPQSAFENPDFKRGLKMFLSPDGKAARFVIALEGDPATPEGISRVEPIKREAREAIKGTPLQGAAIYLGGTAATFKDIREGARYDLLQ"
  },
  {
    "reference": "ALQDDWQQSHLERRQVLAESQKQIQATIGALXXERQXXA",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The designed protein should be expressed specifically in the vacuole The designed protein sequence is ",
    "response#1": "PLTFIQVKQYFGFVLYRTTLPQDCSNPTPLSSPLNGVRDRAYVAVDGVPQGVLERSYVITLNITGQAGATLDLLVENMGRVNYGRYINDFKGLISNLTLGSSVLTDWMIFPLI",
    "response#2": "CEQYLRQQSRDVLQMRGIENPWRREGGSFDECCRELKNVDEECRCDMLEEIAREEQRQARGQEGRQMLQKARNLPSMCGIRPQRC",
    "response#3": "CEQYLRQQSRDVLQMRGIENPWRREGGSFDECCRELKNVDEECRCDMLEEIAREEQRQARGQEGRQMLQKARNLPSMCGIRPQRR"
  },
  {
    "reference": "MVAFGKYLQRKQIEEWSGYYINYKLMKKKVKQYAEQIQGGSQHPRHVLKDFSRMLDTQIETTVLFMLEQQGLLSGRLAKLRESHDAILEQPDISRIFELREAYRDVGRDLLQLLKFVELNAIGLRKILKKFDKRFGYRFADYYVKTRANHPYSQLQQVFKHVGVGAVVGAISRNLHELQENEGSFYSIYDQPVLPAQDPVVEAINNAVDKLTFSTNFLNFLAQHALIMQDDLVTPSEDTIDERSYHFNSLLLNLGNTFLYMVNTYIIVPTADDYSMSLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFSSIALFIGNLMYALAYDANSIALLLLGRVCCGLGSARAVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLLQIKFKFYKFTFNQSTLPGWVMAVAWLFYLVWLCISFREPLRDTEDGEKNNRNETTSDRVESSRVEEGLRLPLLITSGIKPEDEEECDESEESPEDSHKPANSFIEAYRLLTPSVKVQLLIYFMLKYSMEILLSESSVITSYYFSWTTSSVAIFLACLGLTVLPINILVGSYISNMFEDRQILLTSEIIVFLGILFSFNLFVPYTVPQYVISGLIMFVAAEVLEGVNLSLLSRVMSSRLSKGTYNGGLLSTEAGTLARVVADATITLGGYLGRGHLLNATLLPSLVICIGSIVATCCTYNSLY",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The designed protein should have a specific function within the membrane.2. The presence of SPX domains is critical for the interaction of the designed protein with its intended targets.3. The protein should contain Helical transmembrane regions to facilitate transport across the membrane. The designed protein sequence is ",
    "response#1": "HLHMIAIGGSIGAGFFVGSGGALAKGGPGSLFVDFLIIGIMMFNVVYALGELAIMYPVSGSFYTYSARFIDPAWGFAMGWNYVLQWAAVLPLELTVCGITISYWNSEITTAAWISLFLGVIIIINLFGALGYAEEEFWASCFKLAATVIFMIIAFVLVLGGGPKDGRYHEYWGARYWYDPGAFKNGFKGFCSVFVTAAFSFSGTELVGLAAAESTNPTKNMPGAIKQVFWRITIFYILGLFFVGLLINSDDPALLSSAAYADSKASPFVLVGKYAGLKGFDHFMNLVILASVLSIGVSGVYGGSRTLTALAQQGYAPKLFTYIDKSGRPLPSVIFLILFGFIAYVSLDATGPVVFDWLLAISGLAALFTWGSVCLAHIRFRKAWKYHGHTLDEIPFKAAGGVYGSYLGLFICVIVLMAQFYTAIAAPPGSPGVGTAEDFFKQYLAAPVVLGFWIVGWLWKRQPFLY",
    "response#2": "HLHMIAIGGSIGAGFFVGSGGALAKGGPGSLFVDFLIIGIMMFNVVYALGELAIMYPVSGSFYTYSARFIDPAWGFAMGWNYVLQWAAVLPLELTVCGITISYWNSEITTAAWISLFLGVIIIINLFGALGYAEEEFWASCFKLAATVIFMIIAFVLVLGGGPKDGRYHEYWGARYWYDPGAFKNGFKGFCSVFVTAAFSFSGTELVGLAAAESTNPTKNMPGAIKQVFWRITIFYILGLFFVGLLINSDDPALLSSAAYADSKASPFVLVGKYAGLKGFDHFMNLVILASVLSIGVSGVYGGSRTLTALAQQGYAPKLFTYIDKSGRPLPSVIFLILFGFIAYVSLDATGPVVFDWLLAISGLAALFTWGSVCLAHIRFRKAWKYHGHTLDEIPFKAAGGVYGSYLGLFICVIVLMAQFYTAIAAPPGSPGVGTAEDFFKQYLAAPVVLGFWIVGWLWKRQPFLY",
    "response#3": "STTPPWWAIPPAWSLGAEIQAYFLLPILLTYKMLGLSVFWISYIIYSLANLNIIHSDYFGYRLIPGVIFMFLSGAYLQKIVSGKASRLEMLSLIIIYIISLFWLVFFIIIKGKYGAYTRETLLGLLVGIPLVYTLLKIRRKFYFNDLFGKLSYGIFLSHFLSFWILEFVNLTQNIISMIFLSLIISASVSYLLF"
  },
  {
    "reference": "MSMSYQLKTKKLACQRGDNVLFTDFNFVWNSGDFVQIEGHNGIGKTSLLRILIGLAQQREGEVFWNGIPIQTQRETFQYDLLYLGHHSGIKPELTAWENLKFYQQMSACELGEDKLWQVLETVGLLGREDIPAAQLSAGQQRRIALARLWLSKAPLWILDEPFTAIDKHGVQVLTTLFEQHATQGGIVIFTSHQDVQSRLLKKVRLENYKFTE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should be designed to have high stability and activity towards ATP + H2O + heme b(in) = ADP + H(+) + heme b(out) + phosphate.2. The designed protein have ABC transporter domains with specific sequence motifs that are crucial for its activity.3. For general function, the protein need meet that Part of the ABC transporter complex CcmAB involved in the biogenesis of c-type cytochromes; once thought to export heme, this seems not to be the case, but its exact role is uncertain4. The protein should have a high affinity for ATP.5. A protein that can perform ATP binding, ABC-type heme transporter activity, ATP hydrolysis activity in plasma membrane to support cytochrome complex assembly. The designed protein sequence is ",
    "response#1": "TGDMLQISGPNGCGKTSLLRLLCGLMQPTAGQILLDAQPLGRQPAAPGRKPLWIGHAPAIKDVLTPLENLSWLSALHQPDGADADTIATALDAVGLAGFEDVPCHTLSAGQQRRVALARLYLPGPPLWLLDEPFTALDRQGIEQLEKHLAEHCEHGGMIVMTTHHSLNRLPAGYRDLDLGQWL",
    "response#2": "SGEAVAVVGRNGSGKTSLLRLIAGLLIPAGGTIALDGGDAELTLPEQCHYLGHRDALKPALSVAENLSFWADFLGGERLDAHESLATVGLDHATHLPAAFLSAGQRRRLSLARLLTVRRPIWLLDEPTTALDVAGQDMFGGLMRDHLARGGLIIAATHMALGIDSRELRIGGVR",
    "response#3": "LEARDLYCERDERTLFRGLSFTVDAGEWVQVTGGNGAGKTTLLRLLTGLARPDGGEVYWQGEPLRRVRDSFHRSLLWIGHQPGIKTRLTARENLHFFHPGDGARLPEALAQAGLAGFEDVPVARLSAGQQRRVALARLWLTRAALWVLDEPFTAIDVNGVARLTRRMAAHTAQGGMVILTTHQPLPGAADTVRRLALTGGGAGA"
  },
  {
    "reference": "MVMEQAGTTSYSVVSEFEGTILKNADSFSYFMLVAFEAAGLIRFAILLFLWPVITLLDVFSYKNAALKLKIFVATVGLREPEIESVARAVLPKFYMDDVSMDTWRVFSSCKKRVVVTRMPRVMVERFAKEHLRADEVIGTELIVNRFGFVTGLIRETDVDQSALNRVANLFVGRRPQLGLGKPALTASTNFLSLCEEHIHAPIPENYNHGDQQLQLRPLPVIFHDGRLVKRPTPATALIILLWIPFGIILAVIRIFLGAVLPLWATPYVSQIFGGHIIVKGKPPQPPAAGKSGVLFVCTHRTLMDPVVLSYVLGRSIPAVTYSISRLSEILSPIPTVRLTRIRDVDAAKIKQQLSKGDLVVCPEGTTCREPFLLRFSALFAELTDRIVPVAMNYRVGFFHATTARGWKGLDPIFFFMNPRPVYEITFLNQLPMEATCSSGKSPHDVANYVQRILAATLGFECTNFTRKDKYRVLAGNDGTVSYLSLLDQLKKVVSTFEPCLH",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The protein should contain a Helical membrane-spanning region.2. The designed protein should have glycerol-3-phosphate 2-O-acyltransferase activity, phosphatase activity, glycerol-3-phosphate O-acyltransferase activity, sn-1-glycerol-3-phosphate C16:0-DCA-CoA acyl transferase activity to facilitate suberin biosynthetic process, cutin biosynthetic process, CDP-diacylglycerol biosynthetic process.3. Incorporating a HXXXXD motif motif is critical for the successful design of the protein.4. The protein design should take into account the physiological conditions of the CDP- diacylglycerol from sn-glycerol 3-phosphate: step 1/3, CDP-diacylglycerol biosynthesis, Phospholipid metabolism pathway.5. The protein must exhibit the following characteristics: Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis..6. The protein have enhanced an acyl-CoA + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-   phosphate + CoA. The designed protein sequence is ",
    "response#1": "FWNIQIHRGQIEMVKAATEMNLPLIFLPVHKSHIDYLLLTFILFCHNIKAPYIAAGNNLNIPIFSTLIRKLGGFFIRRKLDQSPDGRKDFLYRALLYVHIEELLRQQQFLEIFLEGTRSRSGKTSGARAGLLSVVVDALFSNATPDVLIIPVGISYDRIIEGHYNSEQLGKPKKNESLWSIARGVFRMLRKNYGCVRVDFAQPFSLKEYVFWNIQIHRGQIEMVKAATEMNLPLIFLPVHKSHIDYLLLTFILFCHNIKAPYIAAGNNLNIPIFSTLIRKLGGFFIRRKLDQSPDGRKDFLYRALLYVHIEELLRQQQFLEIFLEGTRSRSGKTSGARAGLLSVVVDALFSNATPDVLIIPVGISYDRIIEGHYNSEQLGKPKKNESLWSIARGVFRMLRKNYGCVRVDFAQPFSLKEYVEVKHADKVRALAQNGHEIIYVPCHRSHMDYLLLTYVIYHEGLVTPHIAAGINLNFWPIGGILRKCGAFFLRRSFAGNKLYTAVFREYLELLFNKGYSVKYYPEGGRSRTGRLLPPKTGMLAMTLQGLIKGINRPVSIVPVYYNGIEVKHADKVRALAQNGHEIIYVPCHRSHMDYLLLTYVIYHEGLVTPHIAAGINLNFWPIGGILRKCGAFFLRRSFAGNKLYTAVFREYLELLFNKGYSVKYYPEGGRSRTGRLLPPKTGMLAMTLQGLIKGINRPVSIVPVYIGYEHVMEVSSYLKELKGTDKKKESFFQVFSAVRKLKNYGNGFLNFGDPINLSNFLPP",
    "response#2": "NLPLIFLPVHKSHIDYLLLTFILFCHNIKAPYIAAGNNLNIPIFSTLIRKLGGFFIRRRLDENPNGRKDVLYRALLYVHVEELLRQQQFLEIFLEGTRSRSGKTSCARAGLLSVVVDALFTNATPDILIIPVGIHVRNAERVRQLAEEGHEIVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTIFREYLGELFTRGYSVEYFVEGGRSRTGRLLEPKTGTLTMTIQAMLRGGNRPITLVPIYIVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTIFREYLGELFTRGYSVEYFVEGGRSRTGRLLEPKTGTLTMTIQAMLRGGNRPITLVPIYVGYGSDGYNGIEVKHADKVRALAQNGHEIIYVPCHRSHMDYLLLTYVIYHEGLVTPHIAAGINLNFWPIGGILRKCGAFFLRRSFAGNKLYTAVFREYLELLFNKGYSVKYYPEGGRSRTGRLLPPKTGMLAMTLQGLIKGINRPVSIVPVYIGYEHVMEVSSYLKELKGTDKKKESFFQVFSAVRKLKNYGNGFLNFGDPINLSNFLYQGLHVRNAERVRQLAEEGHEIVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTIFREYLGELFTRGYSVEYFVEGGRSRTGRLLEPKTGTLTMTIQAMLRGGNRPITLVPIYVGYEHVMEVATYAKELRGAAKEKEGLWQMMRGLRKLRNLGQGYVNFGDPLPLATW",
    "response#3": "YLKELKGTDKKKESFFQVFSAVRKLKNYGNGFLNFGDPINLSNFLDSEVPDWREAQNLEPDKKPRWLTPAVNTLANDVMGRINQAAAVSGMSLCAMCLLSAKKHAMAQDELERAIDDYLDLLKAAPYSDLSSIPELDGKALVENTLKLNKLEVSQDSFGTIISLKRKNAVALTYYRNNILHLFALPGLVSAIVFAHKGLARPQVISLVGQLYPLLQRELFIYMSHEEAMNYTDRLLSTMIDIGLLRAEDDTLCPPAATSKAFYSFWLLNRSIQETLQRYAAVLTILKKEQTIGRGRLEKQSREFAERLAALHGINSPEFFDKNVLSTFILIFLPVHKSHIDYLLLTFILFCHNIKAPYIAAGNNLNIPIFSTLIRKLGGFFIRRKLDQSPDGRKDFLYRALLYVHIEELLRQQQFLEIFLEGTRSRSGKTSGARAGLLSVVVDALFSNATPDVLIIPVGISYAQDGHEIVYVPCHRSHMDYLLLSYVLYHEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNRLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPAKTGMLAMTIQAMLRGLNRPVTLVPVYYNNEQLGKPKKNESLWSVARGVFRMLRKNYGCVRVDFAQPFSLKEYLDSQSQKPMPAILSLEQALLPAILPSRPNDMVDEVSEAALGDSRDLSTEPFRRQLIANLAEHILFTANKSCAVMSTHIVACLLLYRHREGVDLSKLVEDFFSMKEEVLARDFDLGFSGNSEDVVMHAIHLLGNCVNITNTNQNNEFFISNGVLHVFIKEAIIACSLHAVQSKKYRNGINGISPNMISQEQLVRKAASLCHLLSNEGAISLPCQMLYQVCHEAVERFIQYGILLVAEQDDQEDISPSLTEQQWGKKLPEPLSWRSDEEDEDSDFGEEQRDCYLKVNQSQEHQQYITFLQRLLGPLLEAYSSAAIFIHNFSGPVSETEYLQKLHKYLINRTER"
  },
  {
    "reference": "MTASAPRRPAAFLDRDGVINYNDHYVGTRERLRWMPGIAAAIRQLNAAGYYVFIITNQSGVARGMFSEDDVRALHRWMLDELNTQGARIDDVRFCPHHVEGTLDAYRVACEHRKPGPGMILDLAKTWPVDMTRSFVIGDSASDVEAAKAAGIPGFRFEGEDIDVFVKQVLIEMQRAAVSN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein must have a Nucleophile, Proton donor that is structurally stable and resistant to denaturation.2. The substrate binding site should be stable and able to withstand changes in environmental conditions.3. The protein design should consider the endogenous proteins and enzymes present in the Bacterial outer membrane biogenesis, LPS core biosynthesis pathway to avoid interference with native metabolic processes. The designed protein sequence is ",
    "response#1": "VLYVLALTQTRATLLLFPIICVAALIAYYNKSPKKFTSSIVLLIAILASIVIIFNKPIQNRYNEALNDLNSYTNANSVTSLGARLAMYEIGLNIFIKSPFSFRSAESRAESMNLLVAEHNRLRGALEFSNVHLHNEIIEAGSLKGLMGIL",
    "response#2": "VLYVLALTQTRATLLLFPIICVAALIAYYNKSPKKFTSSIVLLIAILASIVIIFNKPIQNRYNEALNDLNSYTNANSVTSLGARLAMYEIGLNIFIKSPFSFRSAESRAESMNLLVAEHNRLRGALEFSNVHLHNEIIEAGSLKGLMGIR",
    "response#3": "VLYVLALTQTRATLLLFPIICVAALIAYYNKSPKKFTSSIVLLIAILASIVIIFNKPIQNRYNEALNDLNSYTNANSVTSLGARLAMYEIGLNIFIKSPFSFRSAESRAESMNLLVAEHNRLRGALEFSNVHLHNEIIEAGSLKGLMGIR"
  },
  {
    "reference": "MTKLDSIEDAILAIREGKQVVVVDDEDRENEGDLILAAVKSTPEAIGFMNRYTSGVICVPMEGEALDRLGLPPMTHINEDRKGTAYSVSVDARDGIETGISAADRSRTIRVLCDSATEPFELTRPGHVFPLRAMPGGVLRRPGHTEAAVDLARLAGLTPAGVIAEVVHDDGSMMRAPALREFADQHGLVMVSIADLIAYRRKNESLVELVATAQLPTEFGVFDICGYQGILDGLDHVAMRIGELGDGEDVLVRVHSECLTGDALGSLRCDCGPQLKAAMRAVAQEGRGIVLYMRGHEGRGIGLLHKLRAYALQDGGSDTVQANLELGLPADSRDYGTGAQILVDMGVKSMRLLTNNPTKRAGLEGYGLTITEQVPIEIEPNDFNMAYLTAKRDSMGHDLHFGDVK",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The Nucleophile; for GTP cyclohydrolase activity, Proton acceptor; for GTP cyclohydrolase activity of the protein must be engineered to enable effective catalysis of GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate.2. A protein that can modulate the overall flux of metabolites through the 5-amino-6-(D- ribitylamino)uracil from GTP: step 1/4 pathway, enhancing or suppressing its activity as needed.3. The designed protein should be able to compete with endogenous proteins or inhibitors for Zn(2+), GTP binding.4. A protein with zinc ion binding, 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, magnesium ion binding functions is required to enable riboflavin biosynthetic process.5. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..6. The designed protein must have at least one GTP cyclohydrolase II domain. The designed protein sequence is ",
    "response#1": "VSVQMPTKYGNFELIAFKQVNTGEIHMALKKGEWEKGEPVLVRVHSSCVTGDILHSLRCDCGEQLAAAMKMVEKEGKGLILYMNQEGRGIGLMNKLKAYKLQEEGRDTVEANLELGFQMDERDYGVGAQILRHLNICKLRLITNNPRKRAGLLGYGLEVVENVEANFPTRFGAFRLYLFKDKLSGEEHIALIKGDIKGKKNVLVRVHSQCFTGDVLGSLRCDCRDQMEKALKIINDEGQGVFLYMRQEGRGIGLSNKVHAYHLQDLGYDTVEANCRLGFEPDLRDYGIGAQILSELGLSSIRLLTNNPKKIVGLEGYGLKVTERVPDRYAVEAVAKLPTKYGDFTIYGYKNITNGEHHVALTMGDVADGEPVLCRVHSECLTGDVFGSKRCDCGEQLDSALKMIADEGRGVLLYMRQEGRGIGLLNKLKAYALQEDGYDTVEANIKLGFPPDLREYGIGAQILHHLGVKRLRLMTNNPTKIAGLSGYGITIEERVREAVAKMPTKYGNFNIVGYQNKTTGEHHVALTMGDVGNGEPVLCRVHSECLTGDVFGSKRCDCGQQLEKAMEMVAKEGRGIILYLRQEGRGIGLINKLKAYELQEQGLDTVDANLKLGFPADMRSYGMGAQILADLGAKKLRIMTNNPAKITGLSGYGIEIVERVPDQYAVGVRKETVKLPTEFGIFDLHCFVAKNDGREHLALVYGEVAGQENVLTRVHSECLTGDVFHSRRCDCGQQLAAAMRQVVANGSGVVVYMRQEGRGIGLVNKLHAYRLQEKGLDTVDANIKLGFPPDLREYGIGAQILANLGVKSIRLLTNNPKKIVGLQGYGLQVTGR",
    "response#2": "ISKARLPTAHGEFTAFVYESKLDGIQHMALIKGDVANKTNVLVRVHSECLTGDVFGSARCDCGSQLDLAMQKIASEGSGVILYLRGHEGRGIGIAHKLRAYQLQDEGMDTVEANVELGLPIDSREYGIGAQILTDLGLTTIRLMTNNPAKYGGLSGYNLTIAERVPFIETPDFRTEAVAKLPTKYGDFTIYGYKNITNGEHHVALTMGDVADGEPVLCRVHSECLTGDVFGSKRCDCGEQLDSALKMIADEGRGVLLYMRQEGRGIGLLNKLKAYALQEDGYDTVEANIKLGFPPDLREYGIGAQILHHLGVKRLRLMTNNPTKIAGLSGYGITIEERVEANFPTRFGAFRLYLFKDKLSGEEHIALIKGDIKGKKNVLVRVHSQCFTGDVLGSLRCDCRDQMEKALKIINDEGQGVFLYMRQEGRGIGLSNKVHAYHLQDLGYDTVEANCRLGFEPDLRDYGIGAQILSELGLSSIRLLTNNPKKIVGLEGYGLKVTERVPSLPRYEDV",
    "response#3": "RAADVLLPTDFGEFRAIAFENDVDRLEHLALVKGEIRGDEPVLVRVHSECMTGDVFGSLRCDCGSQLHRAMEMIEAEGRGVILYMRQEGRGIGLFNKLKAYALQDEGKDTVEANLALGFKPDLRDYGIGAQILVNLGVRNIRLLTNNPRKLIGLQGYGINIVERVPASEVLFDDRVAEATLPTKYGGEFKIIVYENEVDDMKHIALVKGDIQPEDEVLVRVHSECVTGDLFGSLRCDCGDQLHKAMEMVGQEGKGVIVYMHQEGRGIGLANKIRAYDLQEQGRDTVEANIELGFKEDLRDYGIGAQILVDLGVRKMRMITNNPKKIVGVEGYGLTVVERVPRLPMNSHEPGEAVAKLPTKYGDFTIYGYKNITNGEHHVALTMGDVADGEPVLCRVHSECLTGDVFGSKRCDCGEQLDSALKMIADEGRGVLLYMRQEGRGIGLLNKLKAYALQEDGYDTVEANIKLGFPPDLREYGIGAQILHHLGVKRLRLMTNNPTKIAGLSGYGITIEERVPAEELVFDDAGDAEVSTRLPTDYGYFRVLGYRHQIDGTAHVALVAGDVDKLRGAEDVLVRVHSECLTGDVFGSRRCDCGSQLQASMREIQENGSGVIIYLRGQEGRGIGLLKKLEAYRLQDEGLDTVDANLEQGLPEDAREYSVAGQILRDLGIKSVNLLTNNPHKGEGLVGFGVPATEVVFADEANFPTRFGAFRLYLFKDKLSGEEHIALIKGDIKGKKNVLVRVHSQCFTGDVLGSLRCDCRDQMEKALKIINDEGQGVFLYMRQEGRGIGLSNKVHAYHLQDLGYDTVEANCRLGFEPDLRDYGIGAQILSELGLSSIRLLTNNPKKIVGLEGYGLKVTERV"
  },
  {
    "reference": "MEFTAVPDAIEDIKAGKFIIIVDDENRENEGDLAIAAEKVTPEAINFMAKHARGLICLPIIGQRLDELRVPLMVQDNTAKFSTAFGVSVEAKHKTSSGISAHDRAATVKAVLDSKTEPEDLARPGHMFPIRAREGGVLVRAGHTEAIVDLASFAGLYPAGVICEIMNEDGTMARLPQLETMADKFGIKIVSIADLIAYRRRNEKLVEKVTEAKLPTRHGDFVVIAYRSKVDTDEHVALVRGDISGDKPVLVRVHSECITGDVFGSLRCDCGDQIALAMEKIAAEGRGVFLYMRQEGRGIGFHNKLRAYALQDQGLDTVEANIALGFDADLRDYGIGAQILADLGLHKIRLLTNNPKKVIGLESYGLKIVETVDLRTQPTPYNLEYLKTKQKKLGHLLEVDESTEA",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The presence of GTP cyclohydrolase II domains is critical for the interaction of the designed protein with its intended targets.2. The desired protein should be able to perform GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-   pyrimidine + formate + 3 H(+) + 2 phosphate3. The protein must exhibit the following characteristics: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate..4. The designed protein should interact with enzymes and substrates within the Cofactor biosynthesis, riboflavin biosynthesis pathway to enhance or regulate its activity.5. The designed protein should be optimized for 3,4-dihydroxy-2-butanone-4-phosphate synthase activity, magnesium ion binding activity in order to improve riboflavin biosynthetic process.6. The Zn(2+), GTP, Mg(2+), D-ribulose 5-phosphate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "GVDMPTIWGHFDLIAFKQIDTDETHLALVKGTWSPDEPVLVRVHSSCVTGDIFGSCRCDCGGQLHAALEMVEREGKGVVLYMNQEGRGIGLLNKLKAYKLQEMGRDTVEANLELGLPMDARNYGVGAQILRDLSVHKIRLISNNPKKRAGLMGYGLEIIESVPYLDGMPVKLPTEHGEFEFIPFRQTSNGLEHAALVKGKWTRDEPVLVRLHSSCFTGDIFGSLRCDCGSQLHKAMEMVEAAGKGVVIYLSQEGRGIGLFNKIKAYKLQDQGMDTVQANIKLGFGEDERDYGVGASIMRELGLGKIRLISNNPVKRAGLEGYGIKIVEAVPYVEGM",
    "response#2": "GVDMPTIWGHFDLIAFKQIDTDETHLALVKGTWSPDEPVLVRVHSSCVTGDIFGSCRCDCGGQLHAALEMVEREGKGVVLYMNQEGRGIGLLNKLKAYKLQEMGRDTVEANLELGLPMDARNYGVGAQILRDLSVHKIRLISNNPKKRAGLMGYGLEIIESVPNHIEKGTRVAEATLPTKYGGEFKIIVYENEVDDMKHIALVKGDIQPEDEVLVRVHSECVTGDLFGSLRCDCGDQLHKAMEMVGQEGKGVIVYMHQEGRGIGLANKIRAYDLQEQGRDTVEANIELGFKEDLRDYGIGAQILVDLGVRKMRMITNNPKKIVGVEGYGLTVVERVGVDMPTQWGDFSLIAYTQVATGDTHLALVKGEWAPGEPVLTRVHSSCVTGDIFGSCRCDCGGQLHRAMTMVEEAGKGVVLYMFQEGRGIGLLNKLKAYKLQEMGRDTVEANLELGFKMDERDYGVGAQILRDLGVSKLRLMSNNPKKRNALAGYGLEITETVPSEIL",
    "response#3": "TTRIPLQQHGDFNMTVFENDLDPAEHFALVKTPLFANQVPLVRIHSECITGDVFGSCKCDCGKQLESSLALIAAEGGVLVYLRQEGRGIGLANKLKAYALQEAGLDTVEANLQLGLPADDRDYAIAYQILKHLGIDTVRLITNNPSKIKALEEYGICVSERIPLYHEDAPPKRVAEATLPTKYGGEFKIIVYENEVDDMKHIALVKGDIQPEDEVLVRVHSECVTGDLFGSLRCDCGDQLHKAMEMVGQEGKGVIVYMHQEGRGIGLANKIRAYDLQEQGRDTVEANIELGFKEDLRDYGIGAQILVDLGVRKMRMITNNPKKIVGVEGYGLTVVERVSPRMPTPFGEFHVYAYQSDITGEEHVALVMGEIADGEAVLVRVHSSCLTGDVFQSARCDCGHQLERAFELIAAEKKGVILYLLQEGRGIGLVNKLKAYELQDQGHDTIEANAKLGFAPDIRDYGTGSQILRDLGVRKIRLMTNNPAKYVAIEGFGLEIVERVPLYHEEAPPKVEGKDVKLKITIDNVVRELVESKLREIVEKYGGRLA"
  },
  {
    "reference": "MGAVMSMLTDKKQRKALQDACDWLRATVPAGATLHADSRRVQAGDVFVAYAVEGADSRGFIADAVERGAAAVVWQTDGFHWPDGLDDVAAFGVAHLDQLAGPLAALWYGEPSDTLLTLGVTGTNGKTSCSQWLAQLLSKCGMRSAVIGTLGSGFPGHLTVTGFTTPDAVQLQRSLSDLRAQGAAAVAMEVSSHGLYQGRVAGVAFDVAVFTNLTQDHLDYHGTMAAYEAAKTRLFDWPGLKAAVINRDDAMGQRLLARLAGKPQVIEYGIGGAAQSGHGHRVLRAEDVRATSAGTSFTLHLDGASHSLSVPLVGEFNVSNVLAVLGAALAGGVPAARALAALPSLAPVSGRMEQIGQAGQPLVVIDYAHTPDALEKTLAALRPVAQARAGALVCVFGCGGDRDNGKRPLMGAVAERLADAVVLTSDNPRTEAPATILEQIAAGLQAPERARLIEDRASAILQAIRGANASDVVLIAGKGHENTQETMGKKRPFSDQEHAQLALAARATTSRGGGE",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein have Mur ligase central, Mur ligase C-terminal domains with specific sequence motifs that are crucial for its activity.2. The protein should have a high affinity for meso-2,6-diaminoheptanedioate, ATP, UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate.3. The designed protein should have UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity, ATP binding to facilitate regulation of cell shape, peptidoglycan biosynthetic process, cell wall organization, cell cycle.4. The protein must bind to Mg(2+) in order to perform its enzymatic function.5. The protein design should include a Meso-diaminopimelate recognition motif motif to ensure proper function. The designed protein sequence is ",
    "response#1": "MKITGLSVDSRKTRPGHIFAALPGTRVHGAEFIQYALRMGAAAVLTDPEGAALAHDILFDHPDVALVVAEDPRAALACAAALFARFASKRPEHLIAIGVTGTNGKTTVAFMDKSILEAAGVTGTNGKTSITWFLRDALNALGHSCGLMGTLGAGLKGQELTTGHTTPDPITLQRALRALCDAGADSVAMEVSSHALDQHRLGDTPVTIAVFSNLSRDHLDYHGDMDTYLVAKAALFTRAELKQAVINNDDPAAPALLARLNDGVRCITFGDQSGATVRCLAFSPHQHGMDVTLSVGGARVEVALPLFGRFNLSNVMAVAGLCIASGVELHAAAAALSQVTGVPGRFERVPNEKNLAVVVDYAHSDDALLNTLKTARELTSGKIITVFGCGGDRDKTKRAPMGAVAGETSDLVIVTSDNPRTENPLQIISEIEAGLKPTNCPYLVISDRREAIHRAIKLARRGDVVLIAGKGHEDYQIIGHTKHHFDDVEEARKALEGRA",
    "response#2": "MIKLKDILYKVSIQSVVGTTDIGVEGITLDSRKVRPNWMFVAIKGLQSDGHDFINKAIELGADCIICEQIPSERHPHVTYVRVNDAAASLGVMAAEFYGNPSQKLKLVGITGTNGKTTTTYLVLALTEANDERAAMLTTVEYRIGERSEPAIRTTPEASDTQRFLREALNENCLTAVMETSSQAIDLHRCDWLRFRVAVFTNLTRDHLDYHLTMENYFDAKKKLFDGRIGEKPAASVINVDDEWGAKLANELKANNQRVVTFAQNKTADLTAENIEVSLLSGTSFDLKTPQGSRKITSPLVGKPHVYNMLAAMATAIELGIDLQTIVAGIENAIVPGRMERVGAGQPFTAIVDYAHKPDAVTAVLTALRPVTAGRLIIVIGAGGDRDHGKRPLMGEAAARHADVVIVTDDNPRSERASSIRTAVMEGAAAGPGLAIEVADRRDAIEHAVAMAHLGDTVVVAGKGHEDYQILKDRTIHFDDKEVVREILKEQG",
    "response#3": "MEITGLSVDSRKTRPGHIFAALPGTRVHGAEFIQYALRMGAAAVLTDPEGAALAHDILFDHPDVALVVAEDPRAALACAAALFYGAPSRRLRLVGVTGTNGKSSICYYIAQLAQSIGLRSGLVGTFGVGPLGNLSEAKQTTPDILSLHLTLMEMAKSGVDLVAFEASSHALDQGRIEGVPFQTAVFSNLTRDHLDYHGDMASYALAKQRLFSFPGVSHSVFCMDDEYAPFMAEAVGNSDCYYYSEQNSLADFYVKDLSFAPSGCHFVLCHPEGESRIRLPLLGRFNVQNALAALAMLAAAGVPLPEAIRSLTQVAGLPGRMEQAGRHASGARIYVDYAHTPDALETVLSAIRPHARQKLSVVFGCGGDRDQGKRKIMGEIAARLADRVYVTDDNPRNENANLIRSQVLQGCPDAVEIGNRAKAIRAAVDALEKGDILLVAGKGHEPYQEVAGVRHPFSDVEEARAALAARGLAS"
  },
  {
    "reference": "MHELSLCESVIETIMDNVRTHHFSQVRRVRLTVGRFAGVETEALRFSFDVVARGTLVEGAELELLEEPGAAWCFDCSETVPLMDRLDPCPRCGGTRLHPTAGTDVMIKDLEVI",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that Required for nickel insertion into the metal center of the hydrogenase.2. The designed protein must possess nickel cation binding to support protein maturation, protein modification process under certain conditions.3. The protein should have a high affinity for Zn(2+). The designed protein sequence is ",
    "response#1": "LSSPGAGKTALIEKMVGDRQKDHPTAVIVGDLATDNDAQRLRSAGAIAIQVTTGNICHLEAEMVAKAAQKLDLDNIDQLIIENVGNLVCPTTYDLGEDLRVVLFSVTEGEDKPLKYPATFKSAQVILVTKQDIAAAVDFDAELAWQNLRQVAPQAQIK",
    "response#2": "LSSPGAGKTALIEKMVGDRQKDHPTAVIVGDLATDNDAQRLRSAGAIAIQVTTGNICHLEAEMVAKAAQKLDLDNIDQLIIENVGNLVCPTTYDLGEDLRVVLFSVTEGEDKPLKYPATFKSAQVILVTKQDIAAAVDFDAELAWQNLRQVAPQAQIR",
    "response#3": "LSSPGAGKTALIEKMVGDRQKDHPTAVIVGDLATDNDAQRLRSAGAIAIQVTTGNICHLEAEMVAKAAQKLDLDNIDQLIIENVGNLVCPTTYDLGEDLRVVLFSVTEGEDKPLKYPATFKSAQVILVTKQDIAAAVDFDAELAWQNLRQVAPQAQIS"
  },
  {
    "reference": "MSALVSDNTDVSKVSILGSESIILGFHLTDYIVRDVVSSIPASNYVLITDTNLAPLYLERYTKALEQATKARIFTRILPPGELSKSRQTKDDIEDWLLSNAVTRDTCFLAMGGGVIGDLVGFIAATFMRGVPFVQIPTTLLAMVDSSIGGKTAIDTPAGKNLIGAFWQPKRIFMDLTFLKTLPEREFANGMAEVIKTAAIWSEPDFVKLENGVESIRKAVLNTDEATSDNQGAKIESRTKEQNLLLDVILASANVKAHVVTHDEREGGLRGLLNFGHSIGHGIEALVSPLLLHGECVSIGCVKEAELARNLGHLNPVSVGRLARCLSSYGLPISLDEKKVKDRIGNLYCHVYDIMQVMKVDKKNQGDKKRIVLLAKIGQTLEPRASVVADIDIEKVLAPEQLVLPVTESPSGPKKSVSLTTPGSKSISNRALLIAALGQGTVRVKNLLHSDDTQFMLAALQNLGAAEFEWEDNGETLVVHGRGGQLQVPNKEIYVGNAGTASRFLTTVLTMIPSASTETSPHAILTGNARMKQRPIAPLLTALKNNGASIVSTEKEGFLPIAVTPNGGFKGGRIELAASISSQYVSSILLCAPYAVEPVELVLTGGQVISQPYIDMTIAMMESFGAKVDRLPNNTYRINQTTYKNPSDYVVESDASSATYPLALAAITGTTCTVTSIGSNSLQGDATFAINVLRPMGCKVEQTETTTTVTGPAIGQLRPLPSIDMETMTDAFLTASILAAVTSGPTDNITRITGIANQRVKECNRIAA",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should contain one or more Shikimate dehydrogenase substrate binding N-terminal, SDH C-terminal, 3-dehydroquinate synthase domains that are essential for its function.2. Zn(2+) is required for the proper folding and stability of the protein.3. The protein must exhibit the following characteristics: The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis..4. A protein that can modulate the overall flux of metabolites through the Metabolic intermediate biosynthesis, chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7, chorismate biosynthesis pathway, enhancing or suppressing its activity as needed.5. The Zn(2+), 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate binding site should be located in a region of the protein that is accessible to the ligand.6. The Proton acceptor; for 3-dehydroquinate synthase activity, Schiff-base intermediate with substrate; for 3-dehydroquinate dehydratase activity, For EPSP synthase activity active site of the designed protein must be optimized for substrate binding and specificity. The designed protein sequence is ",
    "response#1": "MLVPDAEAGKTIQVASGLWARLGDAGFTRSDAIVGLGGGAATDVAGFVAATWMRGIRYVNCPTSLLAMVDASTGGKTGINTDAGKNLVGSFYTPAGVLADTSTLSTLPNDLAIADSRIRCNQAYAVKPGEANKTRSTKADIEDYLLSKGCTRDTVIVAIGGGIIGDMVGFVAATFMRGVRVIQVPTSLLAMVDSSIGGKTAVDTPLGKNFIGAFWQPQFVLVDVKWLETLPRREFVSMALYLGNAGTAARFLTPLVAGVADVGLGEVAKSGFILDGEILKILSDSNNAKLLREFDGDQLENNNELHELLVELIERTVRVKAYHVSSDLKEQGLREFLNYGHTLGHAIEQIEHFRWRHGNAVAVGMVFAAELAQILGYLDTQDVSYHRSLLDSLGLPTSWQSREDADSAFERVLNLMHRDKKGMAEVIKTAAISDEDEFIALEANSEKIMAAVNTDIKQGAARFKDIESILLRVISASAKFKAHVVTIDERETGLRNLLNFGHSIGHAMEAFLTPQVLHGECVAIGMIKEAELSRYLGVLSGVAVGRLQKCLTAYGLPTRLDDDRIRKLSGGKVCTVDGMLKKMGVDKRL",
    "response#2": "MLVPDAEAGKTIQVASGLWARLGDAGFTRSDAIVGLGGGAATDVAGFVAATWMRGIRYVNCPTSLLAMVDASTGGKTGINTDAGKNLVGSFYTPAGVLADTSTLSTLPNDIAPGETSKSRQTKADIEDWLLEQGCTRDTVILALGGGVMGDLLGFVAATYMRGVRFVQIPTSLLAMVDSSIGGKTAIDTPLGKNLIGSFWQPERIYIDLAFLETLNKRQTHLFYAVKPGEANKTRSTKADIEDYLLSKGCTRDTVIVAIGGGIIGDMVGFVAATFMRGVRVIQVPTSLLAMVDSSIGGKTAVDTPLGKNFIGAFWQPQFVLVDVKWLETLPRREFTLIGLDALYLGNAGTAARFLTGMAEVIKTAAISDEDEFIALEANSEKIMAAVNTDIKQGAARFKDIESILLRVISASAKFKAHVVTIDERETGLRNLLNFGHSIGHAMEAFLTPQVLHGECVAIGMIKEAELSRYLGVLSGVAVGRLQKCLTAYGLPTRLDDDRIRKLSGGKVCTVDGMLKKMGVDKGMAEVIKTAAISDEDEFIALEANSEKIMAAVNTDIKQGAARFKDIESILLRVISASAKFKAHVVTIDERETGLRNLLNFGHSIGHAMEAFLTPQVLHGECVAIGMIKEAELSRYLGVLSGVAVGRLQKCLTAYGLPTRLDDDRIRKLSGGKVCTVDGMLKKMGVDKL",
    "response#3": "MLVPDAEAGKTIQVASGLWARLGDAGFTRSDAIVGLGGGAATDVAGFVAATWMRGIRYVNCPTSLLAMVDASTGGKTGINTDAGKNLVGSFYTPAGVLADTSTLSTLPNDLHLSKRNIAAENIAGWAEAIKHGLIMDEPLLEIFEKNQNEVQGLESPIATEVIRRSMAIKAEVVSKDERETLGIRTLLNYGHTVGHALETATGYDQLLHGEAVSIGMCAAAYIAQEVGLVGEEIVERQRSLLEAYSLPTRYQGVDVDQVRQAMAVDKKGMAEVVKTAAIWNEEEFISLESHPAEIFAAIQTPSKNYTGRSKATRSQAQELLLSVIVGSISVKAHIVTIDERETGLRNLVNFGHSVGHAIEAVLTPTILHGECVSIGMVLEGEISRQMGILSQVGVGRLTRCLKSYNLPVSVSDPRIANLPAAKQLTVERLLDIMRIDKKGMAEVIKTAAISDEDEFIALEANSEKIMAAVNTDIKQGAARFKDIESILLRVISASAKFKAHVVTIDERETGLRNLLNFGHSIGHAMEAFLTPQVLHGECVAIGMIKEAELSRYLGVLSGVAVGRLQKCLTAYGLPTRLDDDRIRKLSGGKVCTVDGMLKKMGVDKIKEI"
  },
  {
    "reference": "MKADQKHTPMMQQYLKLKAENPEILLFYRMGDFYELFYDDAKKASQLLDISLTKRGSSNGEPIPMAGVPYHAVEGYLAKLVQLGESVAICEQIGNPATSKGPVERAVVRIVTPGTVTDEALLSERIDNLIASIYHHNGKFGYATLDITSGRFQLCEPETEEAMAAELQRTSPRELLFPEDFEPVNLMASRNGNRRRPVWEFELDTAKQQLNKQFGTRDLVGFGVEGAKLGLCAAGCLIQYVKDTQRTALPHIRSLTMDKQDHSVILDAATRRNLEITQNLGGGTDNTLAEVLDHTATAMGSRMLKRWLHQPMRNISALDQRLDAIGEMKDLALFTELQPTLKQIGDIERILARLALRSARPRDMARLRQAMEYLPELAETLTQLKHPYLTQLAQYASPVDEVSELLERAIKENPPVVIRDGGVIAEGYNAELDEWRDLAAGATEFLDKLEQEERERHGIDTLKVGYNNVHGFFIQVSRGQSHLVPPHYVRRQTLKNAERYIIPELKEHEDKVLSSKSKALAIEKKLWDELFDLLLPYLERLQNIASSVSQLDVLQNLAERADTLDYCRPTMTESAGVQIQAGRHPVVEQVMDEPFIANPIDLNDQRKMLIITGPNMGGKSTYMRQTALIALMAHIGCYVPAESATIGSIDRIFTRIGASDDLASGRSTFMVEMTETANILHNATPNSLVLMDEIGRGTSTYDGLSLAWASAEWLANQINAMTLFATHYFELTELPNQIPTLANVHLDAVEHGDSIAFMHAVQEGAASK",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should have a binding site that is specific to the target ATP ligand.2. A protein with mismatched DNA binding, ATP binding, ATP-dependent DNA damage sensor activity is needed to enhance mismatch repair in a specific context.3. For general function, the protein need meet that It is possible that it carries out the mismatch recognition step The designed protein sequence is ",
    "response#1": "MIITGPNMAGKSALLRQTALIVLMAQAGSFVPATHATIGTVDKVFTRVGASDNLSKGESTFMVEMNETASILNNLSGRSLVLMDEIGRGTSTYDGISIAWAIVEHLHNHPNFRPKTLFATHYHELNQLTEDLKRVKNFNVSVKEAGNKVIFMRTLKPGGSEHSFGH",
    "response#2": "MIITGPNMAGKSALLRQTALIVLMAQAGSFVPATHATIGTVDKVFTRVGASDNLSKGESTFMVEMNETASILNNLSGRSLVLMDEIGRGTSTYDGISIAWAIVEHLHNHPNFRPKTLFATHYHELNQLTEDLKRVKNFNVSVKEAGNKVIFMRTLKPGGSEHSFGS",
    "response#3": "AALTSRDLAANSIGVDLDRGEVHLGGVISLPTYNRGIADHQYLFVNGRPVKDRLLVGALRGAYADLLARDRHPVVALFLDVPTGEVDVNVHPAKTEVRFRDPQLIRGMIVGGLRRAR"
  },
  {
    "reference": "MRXMGIRRNSQHLWKWGTMLLGMLMICSAXENLWVTVYYGVPVWKEATTTLFCASDAKAYKTEVHNVWATHACVPTDPNPQEVVLENVTEDFNMWKNBMVEQMHEDIISLWDNSLKPCVKLTPLCVTLNCTXXXXXXXXXXXXXXXXXXXGEIKNCSFNITTNIRDKMQKEYALFYKVDVVPIDNNNTDNSSYRLISCNTSIITQACPKVSFEPIPIHYCAPAGFAILKCNDKKFXGKGSCTNVSTVQCTHGIRPVVSTQLLLNGSLAEXEIVIRSDNISDNAKXIIVQLXXXVXINCTRPNNNTRKSIPIGPGRAFYATGDIIGDIRQAHCNISKXEWHSTLXQXTQKLRGEFKNKTIVFNQSSGGDPEIVRHTFNCGGEFFYCNSTLLFDSTWWSNGTXLXRTNITGDTITLNCRIKQIINMWQEVGKAMYAPPIRGQIXCSSNITGLLLTRDGGNSSNNBTEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAXFLGFLGAAGSTMGAASITLTVQARTLLSGIVQQQNNLLKAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLDDIWNNMTWMQWEREIDNYTNIIYNLIEESQNQQEKNELELLELDKWANLWNWFDITQWLWYIKIFIMIVGGLVGLRIVFSVLSXVNRVRKGYSPLSXQXRPPVPRGPDRPEETEXXXGERDRDRXGGSVNGFLXLIWVDLRSLFLFSYH",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. For general function, the protein need meet that The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state2. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.3. The protein design should include a YXXL motif; contains endocytosis signal, Di-leucine internalization motif motif to ensure proper function.4. The designed protein must be able to regulate virion attachment to host cell, actin filament reorganization.5. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "MEKMKNCSYNMSTEVKDKKKQVYSLFYELDVVPLGNSSREYRLISCNTSAITQACPKVTFEPIPIHYCAPAGFAILKCKDKNFTGTGLCKNVSSVQCTHGIRPVASTQLLLNGSLAEGGEIMIRSKNITDNVNNIIVQFTKPVQINCTRPNNNTRESVRIGPGQVFFTTGIIGDIRRAYCLINREKWNTTLQEVAQQLSKHFPNKTIVFKNSTGGDLEITTHSFNCGGEFFYCNTTELFNGTWSNGTEWSKGNVSTNGNITIPCRIKQIINMWQRVGKAMYAPPIPGVIRCVSNITGLILTRDGDTSSADETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRAKRRVVQRDKRLLLGATGFIGWPFM",
    "response#2": "MWVAVYYGVPVWKEAKTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEIVLENVTENFNMWKNDMVNQMHQDVISLWDQSLKPCVKLTPLCVTLKCVNVTKNSNATVNSNATVNNNTMGEEIKNCSFNITTELRDKEQKVRALFYRLDIVPLNEGNNSNSSYYRLINCNTSTITQACPKVSFDPIPIHYCAPAGYAILKCNDKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEENVTIRSENMTNNIKIIIVQLKEPVEINCTRPNNNTRKGIGIGPGQTFFATEDIIGDIRQAHCNISGTKWNHTLEQVMEELKKHFPNKTIEFAPSSGGDLEITTHSFNCRGEFFYCNTSKLFKTSVNPNITLTLPCRIKQIINMWQEVGRAMYAPPISGIITCKSNITGLLLTRDGGGQNNGPETFRPGGGNMRDNWRSELYKYKVVEIKPLGIAPSKAKRRVVEREKR",
    "response#3": "MEKMKNCSYNMSTEVKDKKKQVYSLFYELDVVPLGNSSREYRLISCNTSAITQACPKVTFEPIPIHYCAPAGFAILKCKDKNFTGTGLCKNVSSVQCTHGIRPVASTQLLLNGSLAEGGEIMIRSKNITDNVNNIIVQFTKPVQINCTRPNNNTRESVRIGPGQVFFTTGIIGDIRRAYCLINREKWNTTLQEVAQQLSKHFPNKTIVFKNSTGGDLEITTHSFNCGGEFFYCNTTELFNGTWSNGTEWSKGNVSTNGNITIPCRIKQIINMWQRVGKAMYAPPIPGVIRCVSNITGLILTRDGDTSSADETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRAKRRVVQRDKRXLPGRT"
  },
  {
    "reference": "MKLTCVLIIAMLFLIVCQLNTADDSTDKQEYRAVKLRDAMRNFKGSKRNCGEQGEGCATRPCCAGLSCVGSRPGGLCQYD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. Ensure the presence of a signal peptide within the protein.2. The protein can be targeted to extracellular region and perform toxin activity. The designed protein sequence is ",
    "response#1": "NLYQFGKMIFKMTRKSALSYSDYGCYCGWGGKGKPQDATDRCCFVHDCCYGTVNGCDPKLSTYSYSFQNGDIVCGGDDPCLRAVCECDRVAAICFGENMNTYDTKYMLHSLFDCK",
    "response#2": "NLYQFGKMIFKMTRKSALSYSDYGCYCGWGGKGKPQDATDRCCFVHDCCYGTVNGCDPKLSTYSYSFQNGDIVCGGDDPCLRAVCECDRVAAICFGENMNTYDTKYMLHSLFDCR",
    "response#3": "NLYQFGKMIFKMTRKSALSYSDYGCYCGWGGKGKPQDATDRCCFVHDCCYGTVNGCDPKLSTYSYSFQNGDIVCGGDDPCLRAVCECDRVAAICFGENMNTYDTKYMLHSLFDCK"
  },
  {
    "reference": "MNREEQLGVLESLLFAAGDAGLSTEQLTEVMEITHIEALNLLELLSDRYNGSADRGLILLELAGTFQLATKKAHAEFLRKLVEVPSNTVLSQASLETLAIIAYRQPVTRMEVDEVRGVQTDGPIRTLVAKGLVTDKGRVDGAGRAKLYVTTSEFLDAFGLNSLEDLPKLADPATDEPDQNEMDLFFDRFNQSKEQEEE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The protein should be optimized for stability and expression in cytoplasm where it will participate in cell division.2. The protein must exhibit the following characteristics: Participates in chromosomal partition during cell division. The designed protein sequence is ",
    "response#1": "TLRAMETNPAERPSPYFLETTQGGRMTALVRASMIAFMDEFSRFYELADGTLQRAAYFLDRYLSVTPESDDVLQLRLVGATAVFLAAKYEDQYTLRKIDASMVAARCGYTSETRHKMVSCMETEILAALDYNLV",
    "response#2": "TLRAMETNPAERPSPYFLETTQGGRMTALVRASMIAFMDEFSRFYELADGTLQRAAYFLDRYLSVTPESDDVLQLRLVGATAVFLAAKYEDQYTLRKIDASMVAARCGYTSETRHKMVSCMETEILAALDYNLL",
    "response#3": "TLRAMETNPAERPSPYFLETTQGGRMTALVRASMIAFMDEFSRFYELADGTLQRAAYFLDRYLSVTPESDDVLQLRLVGATAVFLAAKYEDQYTLRKIDASMVAARCGYTSETRHKMVSCMETEILAALDYNLF"
  },
  {
    "reference": "MSSSDHIHVPDGLAESYSRSGGEEGRAWIAGLPALVARCVDRWELKRDGGVRSGEASLVVPVLRADGTRAALKLQMPREETTAALIGLRAWGGDGMVRLLDHDEESSTMLLERLDGSRTLASVEDDDEAMGVLAGLLNRLHSVPAPPGLRGLGEIAGAMVEEVPSAVDSLADPEDRSRLRGWASAVAELVGEPGDRVLHWDLHYENVLAAEREPWLAIDPEPLVGDPGFDLWPALDTGWERIEATGDARRVVRRRFDLLTESLELDRGRAAGWTLARLLQNTLWDIEDGLTAIAPSQIAVAEALAKP",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should have a high affinity for streptomycin.2. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for ATP + streptomycin = ADP + H(+) + streptomycin 6-phosphate.3. For general function, the protein need meet that The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation.4. A protein with aminoglycoside 6-kinase activity, ATP binding is needed to enhance secondary metabolic process, protein phosphorylation in a specific context. The designed protein sequence is ",
    "response#1": "KDLLRLLTCGSVDDGKSTLIGRLLYDTRMIYEDQLAAVIKDSLVHGTTGTDFDPALLTDGLKAEREQGITIDVAYRYFSTSKRKFIIADTPGHEQYTRNMATGASNCNLAVVLIDARNGVVTQTKRHSFIVSLLGIRHVLVAINKMDLLDWSQDVYETIRRDYNDFVARLGFGDIHFIPISALHGDNIVTPSPNLPWYRGPTLLHHLENVNIVTDKDLLRLLTCGSVDDGKSTLIGRLLYDTRMIYEDQLAAVIKDSLVHGTTGTDFDPALLTDGLKAEREQGITIDVAYRYFSTSKRKFIIADTPGHEQYTRNMATGASNCNLAVVLIDARNGVVTQTKRHSFIVSLLGIRHVLVAINKMDLLDWSQDVYETIRRDYNDFVARLGFGDIHFIPISALHGDNIVTPSPNLPWYRGPTLLHHLENVNIVTDELKGKQSLYWLALRCLLWPDHEDVHLQEMRQLITQAHRLQLLAYTDTQQAIAMLEASIRYEYVNGTQTSPVAFLEGIFVLPEYRRSGIATGLVQQVEIWAKQFACTEFASDAALDNQISHAMHQALGQ",
    "response#2": "LLRLLTCGSVDDGKSTLIGRLLYDTRMIYEDQLAAVIKDSLVHGTTGTDFDPALLTDGLKAEREQGITIDVAYRYFSTSKRKFIIADTPGHEQYTRNMATGASNCNLAVVLIDARNGVVTQTKRHSFIVSLLGIRHVLVAINKMDLLDWSQDVYETIRRDYNDFVARLGFGDIHFIPISALHGDNIE",
    "response#3": "QMMKHVSSHGIMTNCPVSVVVVDADNKNGDATFNNVDVTLHDLPVVSKEHSPRKLAVRLLSWVDGHTMGSCKCLPIEKMAEAGEFLGKLSIALADMVPGGAAAIPPESALMRFHAWDVQHTDKSVSRFVDYLQDPRKKELVNDVMRALKEDLLPIKDELRVGIIHNDFNDANIILDANGNVNGVIDFGDSLCTWIVADISIAMAKDLLRLLTCGSVDDGKSTLIGRLLYDTRMIYEDQLAAVIKDSLVHGTTGTDFDPALLTDGLKAEREQGITIDVAYRYFSTSKRKFIIADTPGHEQYTRNMATGASNCNLAVVLIDARNGVVTQTKRHSFIVSLLGIRHVLVAINKMDLLDWSQDVYETIRRDYNDFVARLGFGDIHFIPISALHGDNIVTPSPNLPWYRGPTLLHHLENVNIVTDDE"
  },
  {
    "reference": "MRVTGIRKNYQQLWKWGALLLGMLMICSAAENLWVTVYYGVPVWKEATTTLFCASDAKAYATEVHNIWATHACVPTDPNPQEVVLGNVTENFNMWKNDMVEQMHEDIINLWDQSLKPCVKLTPLCVTLNCTNYLGNTNDTSSNNATNTNSSNTTANNNSTSNTTWGGEIKICSFNVTTSIKTKVKDYALFNTLDIEPIENDNTSYRLIRCNTSVITQACPKISFEPIPIHYCTPAGFAILKCNNKTFNGTGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVVIRSSNFTDNAKVIIVQLNKSVKINCTRPNNNTRKSIPLGPGRAWYTSGQIIGDIRKAHCNISRAEWNNTLKQVAKKLRGQFGNKTIVFERSSGGDPEIAMHTFNCGGEFFYCNTSQLFNSNWNESTLNDNSTWNDTTITLPCRIKQIVNMWQEVGKAMYAPPIAGQINCSSDITGLLLTRDGGKKNESNNNTETFRPEGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGTIGAMFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQRNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGRLICTTAVPWNASWSNKSLSEIWDNMTWMEWEREIGNYTREIYNLIEESQNQQEKNELELLELDKWASLWNWFDITQWLWYIKIFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSLQTRLPTQRGPGRPEGIEEEGGEQDRDRSERLVNGFLTL",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein must exhibit the following characteristics: Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.2. A protein that contributes to virion attachment to host cell, positive regulation of plasma membrane raft polarization by functioning in virion membrane with structural molecule activity.3. Incorporating a Di-leucine internalization motif motif is critical for the successful design of the protein.4. The designed protein should contain one or more Human immunodeficiency virus 1 envelope glycoprotein Gp120, Retroviral envelope protein GP41-like domains that are essential for its function.5. Include a signal peptide as an essential component of the protein.6. Target a Basic and acidic residues compositional bias in the protein's composition for improved properties. The designed protein sequence is ",
    "response#1": "MKVKGIRKNYQHLWRWGTMLLGMLMICSAAENLWVTVYYGVPVWKEATTTLFCASDAKAYETEGHNVWATHACVPTDPSPQEVKLENVTENFNMWKNNMVDQMHEDIINLWDQSLKPCAKLTPLCVTLNCTNVNATNTNTNTNNSSGIEGGEMKNCSFNITTGVRDKVQSDYAIFSKLDVVPIDDRDSNTSYRLINCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGKGLCTEVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVIRSENFSDNAKTIIVQLNKSIEINCTRPNNNTRRSIPIGPGRAFFATDIIGDIRQAHCNISRAKWNENLKQIAKKLREQFNKTIVFKQHSGGDPEIVMHSFNCRGEFFYCNTTQLFNSNDTEGSNTTEENITLPCRIKQIINMWQEVGKAMYAPPIRGQISCSSNITGLLLTRDGGADSTNETFRPGGGDMRDNWRSELYKYKVVRIEPLGIAPSKAKRRVVQREKRAVGLGALFIGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTAVPWNASWSNKTYNQIWDNMTWMQWEREIDNYTSIIYDLIEKSQNQQEKNEQDLLELDKWASLWNWFEITSWLWYIKIFIMIVGGIVGLRIIFTVLSIVNRVRQGYSPLSFQTRLPAPRGPDRPEGIEEEGGERDRDRSNRL",
    "response#2": "LWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPIDPNPQEVVLENVTENFNVWKNDMVEQMHEDIINLWDQSLKPCVKLTPLCVTLNCSNLKNDTKANNGSNTNNSSGRVVEKGVMKNCSFNVTTNRRNKPRKEYALFYELDVTQIDNTSYILTHCNTSVITQACPKVSFEPIPIHYCTPAGFAILQCNDKEFNGKGPCSNVSTVQCTHGIRPVVSTQLLLNGSLAEREIVIRSENFTDNAKTIIVQLNASVEINCTRPNNNTRKGIHLGPGKTFFATGEVIGDIRQAHCNISKGKWENTLRQLAKKLREQFKNRTIVFNQSSGGDPEIVMHSFNCGGEFFYCNTTQLFNSTWDFNSTWNSTEGSNITLTLPCRIKQIINRWQEVGKAMYAPPISGQIRCSSNITGLLLTRDGGKTGDNGSEIFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTEARRRVVQREKRAVLGAVFLGFLGAAGSTMGAASMTLTVQARLLLSGIVQQQNNLLRAVEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTNVPWNASWSNKSLNKIWDNMTWMEWDREINNYTNLIYTLLEESQNQQEKNEQELLELDKWASLWNWFDISNWLWYIKIFIMIVGGLVGLRIIFSVLSIVNRVRQGYSPISFQTRLPAQRGPDRPEGIEEEGGERDRDTSGRLVNGFLALIWEDLRSLCLFSYHRLRDLLLIV",
    "response#3": "MRVKGIRKNYRHLWKWGTMLLGMLMICSAAEQMWVTVYYGVPVWKEAKTTLFCASDAKAYEREVHNIWATHACVPTDPNPQEMILENVTENFNMWKNDMVDQMHEDIISLWDESLKPCVRLTPLCVTLNCTNVINDTYTNTNNSSGIEGGEMKNCSFNITTGVRDKVQSDYAIFSKLDVVPIDDRDSNTSYRLINCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGKGLCTEVSTVQCTHGIRPVVSTQLLLNGSLAEEEIVIRSENFSDNAKTIIVQLNKSIEINCTRPNNNTRRSIPIGPGRAFFATDIIGDIRQAHCNISRAKWNENLKQIAKKLREQFNKTIVFKQHSGGDPEIVMHSFNCRGEFFYCNTTQLFNSNDTEGSNTTEENITLPCRIKQIINMWQEVGKAMYAPPIRGQISCSSNITGLLLTRDGGADSTNETFRPGGGDMRDNWRSELYKYKVVRIEPLGIAPSKAKRRVVQREKRAVGIGALFLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQNDLLRAIEAQQHMLQLTVWGIKQRQARVLAVERYLKDQRFLGLWGCSGKTICTTNVPWNSTWSNKSYDEIWNNMTWVEWEKEISNYTNKIYDLLTESQNQQEKNEKDLLELDKWASLWNWFDISNWLWYIKIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTLIPNPRGPDRPEGIEEEGGERDRDRSGPL"
  },
  {
    "reference": "MPKMKTNRGAAKRFKLKKNAIKRGSAFKNHILTKKSHQRKANLNAPKYVHSTNVDSVKSLLCMA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The ideal protein would be one that plays a critical role in translation through structural constituent of ribosome within ribonucleoprotein complex. The designed protein sequence is ",
    "response#1": "LPIGCRVTLRGERMYDFLERLIFIALPRVRDFQGIPRRGFDGNGNYNLGIKEHTIFPEISFDKTDAVKGLNITIVTTADNDDMARTLLERVGLPR",
    "response#2": "MNTVI",
    "response#3": "MLENAS"
  },
  {
    "reference": "MSRDISQVLSSFNVQCASIVVAHLRLDSRQVNEGDAFIALRGHHQNGEDYIPSAFERGAVCVIIDESCDVNVAEQRLIRVPDLSSHIARIAAKFYQYPSKQMCTIGVTGTNGKSTTTTMIANLAKHCAHPSAIIGTLGYGQPDALTPLANTTPSNVDLQRILAQLGQSNELVAMEVSSHGLVQGRLDETAFDVAVFTNLSRDHLDYHTDMTSYAQAKLSLFQHHKPEFSVLNADDEYAKQWLDKYSFNNLVVYGKKPENHAFKNFVWFDGVTCDALGFEVKISSSWGEGKLRAPLYGLFNVYNLVAAIATLLTQGFEFEQLLAASQKLEPVAGRMQAFRADDKPICIVDYAHTPEALELALQALQQHVPGNVMCVFGCGGDRDKGKRPLMAQAVERHADQLVITSDNPRSEDPQKIIDDVKAGLSNPQYAVCKVDRAEAIRYAIEHAPIDGVVLIAGKGHEDYQIIGDQRLPFCDRRYVEAVLREKSA",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be optimized for ATP binding activity in order to improve cell cycle, cell wall organization.2. The protein design must feature the Meso-diaminopimelate recognition motif motif.3. The designed protein should contain one or more Mur ligase central domains that are essential for its function.4. The UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, ATP binding site should be located in a region of the protein that is accessible to the ligand. The designed protein sequence is ",
    "response#1": "VTGTQGKTTTTRLAESALLEAGHAAGVIGTVGTRIGGHDVKTPLTTPEAPDLHGLFARMVEEDTEACAMEVSSHALVMGRVDGVVFDVAVFLNLGRDHLDFHDTVEDYFQAKAALFTPERARMGIVNIDDEHGRRLAEEASIPVVTYALHEESADWHVSGIELRPDGSSFVVHGPDGLELPAASPLAGDYNVSNTLAALALLDAVGVPFAAACAALVTGTQGKTTTTRLAESALLEAGHAAGVIGTVGTRIGGHDVKTPLTTPEAPDLHGLFARMVEEDTEACAMEVSSHALVMGRVDGVVFDVAVFLNLGRDHLDFHDTVEDYFQAKAALFTPERARMGIVNIDDEHGRRLAEEASIPVVTYALHEESADWHVSGIELRPDGSSFVVHGPDGLELPAASPLAGDYNVSNTLAALALLVAAGVEAGAAADAVRTLSGVPGRLERVDAGQPFTVVVDYAHKPDAVEAALGTLRPLTESKLIAVIGAGGDRDSGKRPIMGEISARLADHVIVTDDNPRTEDPASIRRQVLEGTTGARAIVEEIGDRRAAIGRAIELAEPGDIVVVAGKGHEAGQTVAGVVHPFDDAEVARAALQGRQNG",
    "response#2": "VTGTNGKTTIATLLYNMFRKMGHKCGLLSTVCNYIEDEAIPADHTTPDPIELNMLLGRMVEAGCEYAFMECSSHAIAQKRIGGLMFAGGIFTNLTRDHLDYHKTFENYRNAKKAFFDDLPKTAFAITNADDKNGMIMVQNTKATVKTYSETKMADFRGRIIECHFGGMYLDIDGHEVGVQFIGKFNVSNLLAVYGAARAQGAS",
    "response#3": "ITGTSGKTTTTYMVEAGLRAGGRVAGLIGTIGIRIDGADIPSALTTPEAPALQAMLAAMFERGVDTVAMEVSSHALTLGRVDGAKFAVGAFTNLSRDHLDFHPSMADYFEAKASLFNPKSALRARTAVVCVDDEAGRAMADRAGDAITVSATDQPAHWRATDVAPVGAGGQEFTVIDPVGVHHRVGIPLPGRYNVANCVVALAILDELGVATADSRAVKPGFLFAALPGERADGARFVSQAIEAGAAAILIGDQPLEGKISVPLIRSADPRRALALAAARFLAGERAAG"
  },
  {
    "reference": "MNKKNIALIVSGGEGKRFDKKKPKQFFKINEKTILEITVKKFVDSKLLDEIVVVCSKDYLNDTKKVLRNQNVLITLGGKTRQDSVYNGLKFCKKFKPSKIIIHDVVRPFFSVKFLNDILKKIDRKVCVIPVLKIFDSVRSIKSNNYSDINRDNLKLIQTPQGFNYNEIFNAHNRFKNLNITDDSLLAYKNGCRIKVLKGEYFNFKITEKSDLERSKIIIAKNMNNTTRTGSGFDVHKFTKGNFLILFGIKIPFDKSLEGHSDADVGFHSLVDAILGAIGKGDIGEHFPPSEKKWKNKNSLFFLEYAASILDEEKFKINNLDLTIICEKPKISKYKEKMKKKISKALNIKLNKINIKGTTTEKLGFLGREEGIACQANVTVSMKNDFDF",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should contain one or more 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domains that are essential for its function.2. A protein with metal ion binding, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity is needed to enhance isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway in a specific context.3. For general function, the protein need meet that Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D- erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C- methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).4. a divalent metal cation is required for the proper folding and stability of the protein. The designed protein sequence is ",
    "response#1": "MVLNSVETWTGQGFDVHALVPGDHVMLCGVKIAHTRGLKGHSDADAGLHALTDALYGALGEGDIGHHFPPSDPKWRGANSAVFLRAAVALLKARGGKINNLDVTIICEEPKIGPHRETMRQAIATIAEIDRRRVSVKATTSERLGFAGRGEGIAALATATLAAPAKTHPRVGIGTDVHAFGGEGDLWLAGLEWPGEQPLSGHSDGDAVAHAIVDALLGAAGLGDIGEHFGTAHPEYAGGHAEVFLARTRELLAAAGFAIGNVSVQFQGNRPKFSPRRAEAQSRLAEILGAPVSITATTTDGLGFPGRSEGIAVTAVAIVFRTGWGYDVHRYLASNETDAKTKSQARPMMLGGIAIPGGPQVIAHSDGDVLLHALTDALLGVACAGDIGQLFPDTDQAFANMPSAVFVTEALRRVRACGYRPAHADLTVVAQVPKLGPHKARMAAQIAQLLELPQACVNVKATTEEGLGFTGEKRGIKAIAVVE",
    "response#2": "MVLNSVETWTGQGFDVHALVPGDHVMLCGVKIAHTRGLKGHSDADAGLHALTDALYGALGEGDIGHHFPPSDPKWRGANSAVFLRAAVALLKARGGKINNLDVTIICEEPKIGPHRETMRQAIATIAEIDRRRVSVKATTSERLGFAGRGEGIAALATATEARLETILPAVKITTPADLALLSSPMNEAKPPRTGWGYDVHRYLASNETDAKTKSQARPMMLGGIAIPGGPQVIAHSDGDVLLHALTDALLGVACAGDIGQLFPDTDQAFANMPSAVFVTEALRRVRACGYRPAHADLTVVAQVPKLGPHKARMAAQIAQLLELPQACVNVKATTEEGLGFTGEKRGIKAIAVVKP",
    "response#3": "MVLNSVETWTGQGFDVHALVPGDHVMLCGVKIAHTRGLKGHSDADAGLHALTDALYGALGEGDIGHHFPPSDPKWRGANSAVFLRAAVALLKARGGKINNLDVTIICEEPKIGPHRETMRQAIATIAEIDRRRVSVKATTSERLGFAGRGEGIAALATATGWVGDRTGNGFDVHAFGPGDHVTLCGIDIPHTYGLVGHSDADVALHTVTDAIYGALARGDIGQHFPPSDPQWKGARSDIFLRHAAQMARDMGFTLTHVDCTLVCETPKIGPHVEPMRAAMAEMLGIEIDRVSVKATTSERLGFTGRGEGIACMATATLVLEG"
  },
  {
    "reference": "MSNQHSPQPFCLDTKLVKLLEELQEGKQFNNKNIFPEKALYLKLALDYSFFRKNLLEFCVHLDKIKGVIRPNYDTIYILCLLEVDLLNLVFTDNILEICLPRFVSREDLRVFNNTFYTYHDNRLRILQEDFSQLFKKIKTKASVLCFTVEEIFLTNQEILPQNSTVAELQKSTNKVQTNGPQRHDFIVTLEIKLNKTQITFLIGAKGTRIESLREKSGASIKIIPISDKMTAHERNHPESVQQTILISGDLYSIALAVTSIESALITLDL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should have mRNA binding, U1 snRNP binding within nucleus, polysome to regulate nuclear division, reciprocal meiotic recombination.2. For general function, the protein need meet that MER1 may function to bring the axial elements of the synaptonemal complex corresponding to homologous chromosomes together by initiating recombination The designed protein sequence is ",
    "response#1": "KEGFSVFGMLNKCVTPMGKHLLRTWFLRPIIDIDVINNRLNTISFFLCCEDVMSALRGTLKSVRDIPHMLKKFNSPSSFCTSSDWHAFLKCICSLLHINKIFEVGISEHLAIKLQHMNIDLVGKANSSITEELDYVSDLVVGVIDVQRGKEKGYDTLVKDGLCEELDELRMVYEELPDFLEQVSANEIASFPFSFECRKAPLIVYVHQIGYLMCFFDEKISDALLIGLPDFEFAFSEEGEERRFYYHTQKTRELDNLLGDIYHKILDMERAIIRDLVCRVCQFIPQLTKAVNFAAELDCILSLAIVARQNNYVRPILTEDSILEIQNGRHALQEMH",
    "response#2": "KEGFSVFGMLNKCVTPMGKHLLRTWFLRPIIDIDVINNRLNTISFFLCCEDVMSALRGTLKSVRDIPHMLKKFNSPSSFCTSSDWHAFLKCICSLLHINKIFEVGISEHLAIKLQHMNIDLVGKANSSITEELDYVSDLVVGVIDVQRGKEKGYDTLVKDGLCEELDELRMVYEELPDFLEQVSANEIASFPFSFECRKAPLIVYVHQIGYLMCFFDEKISDALLIGLPDFEFAFSEEGEERRFYYHTQKTRELDNLLGDIYHKILDMERAIIRDLVCRVCQFIPQLTKAVNFAAELDCILSLAIVARQNNYVRPILTEDSILEIQNGRHALQEMAEEQSQKLLQTMLTMSFGCLAFLRGLFPDDIFVDQRFVPEKVEKNYNKQNTSQNNSIKIKTLIRGKSAQADLLLDWLEKGVFKSIRLKCLKALSLGIFLEDPTDLLENYIFSFDYDEENNVNINVNLSGNKKGSKNADPENETISLLDSRRMVQQLMRRFIIITQSLEPLPQKKFLTMRLMFNDNVDEDYQPELFKDATFDKRATLKVPTNLDNDAIDVGTLNTKHHKVALST",
    "response#3": "KEGFSVFGMLNKCVTPMGKHLLRTWFLRPIIDIDVINNRLNTISFFLCCEDVMSALRGTLKSVRDIPHMLKKFNSPSSFCTSSDWHAFLKCICSLLHINKIFEVGISEHLAIKLQHMNIDLVGKANSSITEELDYVSDLVVGVIDVQRGKEKGYDTLVKDGLCEELDELRMVYEELPDFLEQVSANEIASFPFSFECRKAPLIVYVHQIGYLMCFFDEKISDALLIGLPDFEFAFSEEGEERRFYYHTQKTRELDNLLGDIYHKILDMERAIIRDLVCRVCQFIPQLTKAVNFAAELDCILSLAIVARQNNYVRPILTEDSILEIQNGRHALQEMM"
  },
  {
    "reference": "MQTTDLGNKESGKIWHRKPNPSTNEGIIVNIVHVDASHPSKSARFLHAGFDSAGDSFIAGDHLGNVYLFNLNRNRFDLVQKTMQACTAIAFNLHRRTEFLVALADNSVKCFDTGAKELVSWMRGHESAVTSISVHPSGRYAVTTSSDTAQLWDLDTFQRKRKLNVRQSVGIQKVFFLPLSNTILSCFKDDSIFAWESDTLACKYQLPIPEDGTKLRYKAFAITRDGRMLAAGGKSNNLHLWCLDSKQLFRIIQMPTKVRSVQQLEFLPENFDGGSSQILGVLSQDGIMRFINIQTCKLVFDIGSHDNGIVTSSVSPNGRYITSVMENGSLNIYSVQALSKELNKPPPPLVKMVDLSKDKDSTGNKSGVSGASQEKVRVSSGRTCRPWKSKDQIVRTKYLRPEDTTTSEDKENTLPAGLSKQRLQALLKGFGEYPAKYRMFIWRSLLQLPENHAAFSSLLDKGTHTQYKLLHQEYPIKSRKLLRVLQRTLSALAHWSAIFGETKYLPLLAFPFVKLFQNNQLICFEVVATVITNWCQHWFEYFPNPPINILGMVENLLAHHDKELLQHFINYGVTSQVYAWPLLETLFSEVLTREEWLRLFDNVFSNHPSFLLMAVVSYIISSRSPLLHCNQKDDFEYFFHHRNNLDIGNMIREAYHLMDTSPAEIHPRRLLSDFEPLTRGQYPIFNKYPKFIVDYQGQERERIRQEEIEYLRERQLTHKVEAEAVKRRLEDEAWYQQQELLKGAEEQRRKLLMDEEQKLLHQRQRLAT",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should have a specific function in cilium assembly and should be localized to ciliary basal body in order to carry out this function efficiently.2. For general function, the protein need meet that Part of a functional complex including OFD1 a centriolar protein involved in cilium assembly3. The designed protein must have at least one Rab-GAP TBC domain. The designed protein sequence is ",
    "response#1": "KLQLILDQLDPGQPKEVRYEALQTLCSAPPSDVLNCENWTTLCEKLTVSLSDPDPVFSDRILKFCAQTFLLSPLHMTKEIYTSLAKYLESYFLSRENHIPTLSAGVDITNPNMTRLLKKVRLLNEYQKEAPSFWIRHPEKYMEEIVESTLSLLTVKHNQSHVVSQKILDPIYFFALVDTKAVWFKKWMHAHYSRTTVLRLLETKYKSLVTTAIQQCVQYFEMCKTRKADETLGHSKHCRNKQKTFYYLGQELQYIYFIHSLCLLGRLLIYKQGRKLFPIKLKNKKGLVSLIDLLVLFTQLIYYSPSCPKMTSAAHSENYSPASMVTEVLWILSDQKECAVECLYNNIVIETLLQPIHNLMKGNEASPNCSETALIHIAGILARIASVEEGLILLLYGANMNSSEESPTGAHIIAQFSKKLLDEDISIFSGSEMLPVVKGAFISVCRHIYSTCEGLQVLITYNLHESIAKAWKKTSLLSERIPTPVEGSDSVSSVSQESQNIMAWEDNLLDDLLHFAATPKGLLLLQRTGAINECVTFIFNRYAKKLQVSRHKKFGYGVLVTRVASTAAGGIALKKSGFINELITELWSNLEYGRDC",
    "response#2": "WGYCSNTVVYAYTRPDRPEYCVVFWDTKNNEKYVKYVKSLISITTCGDFCILATKADENHPQFVLVLCNSIGTPLDPKYIDLVPLFVAMTKTHVIAASKEALYTWQYRVAKKLTALEINQITRSRKEGRERIYHVDDVPSGSVDGVFDYSKAIQGTRDPICAITASDKTLIVGRESGVIQRYSFPNVALIQKYSLDCRACQLSLNCNSSRLAIIDIAGVLTFFDLDTRVTDSTGQQVVGELLKLERKDVWDMKWAKDNPDLFAMMEKTRMYVFRNLDPEEPIQTSGYICNFEDLEIKSVLLDEILKNPEHPSKDYIMNFR",
    "response#3": "YPIKSRKLLRVLQRTLSALAHWSAIFSDTPYLPLLAFPFVKLFQNNQLICFEVVATLIINWCQHWFEYFPNPPINILSMIENVLAFHDKELLQHFIDRDITSQVYAWPLLETLFSEVLTREEWLRLFDNIFSNHPSFLLMTVVAYF"
  },
  {
    "reference": "MATLEKLMKAFESLKSFQQQQQQQQQQPPPPPPPQAQVRRPPPPPEERLLLASSVPPPAPPPAPPPPARLHGPPGAGKKELSATKKDRVNHCLTICESIVGQSLRNSPEFQKLLGIAMELFLLCSDDAESDVRMVADECLNKVIKALMDSNLPRLQLELYKEIKKNGAPRSLRAALWRFAELAHLVRPQKCRPYLVNLLPCLTRTSKRPEESVQETLAAAIPKIMASLGNFANDNEIKVLLKAFITNLKSSSPTTRRTAAACVVSICQHSRRTQYFYSWLLSVLLGLLVPVEGEHPSLLVLGALLTLRYLVPLLQQQVQDTSLKGSFGVTRKEMEVSPSTEQLVQVYELTLHYAQHPDHNVVTGALELLQQLLRTPPPELLRALTTPGGLAQLSAPRQEPGSRSRSGSIVELIAGGGSSCSPVLSRKQKGKVLLGEAAALEEDSESRSEGSSPAFAASVKGEICGELAASSGVSTPGSVSSAADSTGHDIITEQPRSQHTLQPDSVDLTGCDLASAATDGDEEDILSHSSSQISAVPSDPAMDLNDGTQASSPLSDSSQTTTEGPDSAVTPSDSSEIVLDGADGQDPGMQLPLPEEEEGEEEAEEEEEPAGLPEDSVAFAAASAALQQAHLLKSMGHSRQPSDSSVDRGVSREDAADPGDQDNKPCRIRGDIGQPDDDDAAPLTHCVRLLSASFLLTGERNALVPDRDVRVSVKALALSCVGAAVALYPESFFSKLYTAPLDAAECPEEQYVSDVLSYIDHGDPQVRG",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. For general function, the protein need meet that May play a role in microtubule-mediated transport or vesicle function.2. A protein that can perform identical protein binding, kinase binding, dynactin binding, p53 binding, dynein intermediate chain binding in nucleoplasm, dendrite, late endosome, cytoplasmic vesicle membrane, centriole to support negative regulation of extrinsic apoptotic signaling pathway, vocal learning, positive regulation of lipophagy, positive regulation of apoptotic process, regulation of cAMP-dependent protein kinase activity.3. The protein should exhibit Basic and acidic residues, Acidic residues, Pro residues, Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "VLDWVHGRISNFHYLMQLNRLAGRRQGDPNYHPVLPWVVDFTTPHGRFRDLRKSKFRLNKGDKQLDFTYEMTRQAFVAGGAGGGEPPHVPHHISDVLSDITYYVYKARRTPRSVLCGHVRAQWEPHEYPASMERMQNWTPDECIPEFYTDPSIFRSIHPDMPDLDVPAWCSSSQEFVAAHRALLESREVSRDLHHWIDLTFGYKLQGKEAVKEKNVCLHLVDAHTHLASYGVVQLFDQPHPQN",
    "response#2": "VLDWVHGRISNFHYLMQLNRLAGRRQGDPNYHPVLPWVVDFTTPHGRFRDLRKSKFRLNKGDKQLDFTYEMTRQAFVAGGAGGGEPPHVPHHISDVLSDITYYVYKARRTPRSVLCGHVRAQWEPHEYPASMERMQNWTPDECIPEFYTDPSIFRSIHPDMPDLDVPAWCSSSQEFVAAHRALLESREVSRDLHHWIDLTFGYKLQGKEAVKEKNVCLHLVDAHTHLASYGVVQLFDQPHPQCL",
    "response#3": "DWVHGRISNFHYLMQLNRLAGRRQGDPNYHPVLPWVVDFTTPHGRFRDLRKSKFRLNKGDKQLDFTYEMTRQAFVAGGAGGGEPPHVPHHISDVLSDITYYVYKARRTPRSVLCGHVRAQWEPHEYPASMERMQNWTPDECIPEFYTDPSIFRSIHPDMPDLDVPAWCSSSQEFVAAHRALLESREVSRDLHHWIDLTFGYKLQGKEAVKEKNVCLHLGLTGNLFDVFLKPYFLEAYRPLHKGDIFTVQAAMRTVEFKVVETEPAPACIVSPDTMIHYEGDPIKRDWVHGRISNFHYLMQLNRLAGRRQGDPNYHPVLPWVVDFTTPHGRFRDLRKSKFRLNKGDKQLDFTYEMTRQAFVAGGAGGGEPPHVPHHISDVLSDITYYVYKARRTPRSVLCGHVRAQWEPHEYPASMERMQNWTPDECIPEFYTDPSIFRSIHPDMPDLDVPAWCSSSQEFVAAHRALLESREVSRDLHHWIDLTFGYKLQGKEAVKEKNVCLHLDWVHGRISNFHYLMQLNRLAGRRQGDPNYHPVLPWVVDFTTPHGRFRDLRKSKFRLNKGDKQLDFTYEMTRQAFVAGGAGGGEPPHVPHHISDVLSDITYYVYKARRTPRSVLCGHVRAQWEPHEYPASMERMQNWTPDECIPEFYTDPSIFRSIHPDMPDLDVPAWCSSSQEFVAAHRALLESREVSRDLHHWIDLTFGYKLQGKEAVKEKNVCLHLGQPTGQEELRSLVLDWVHGRISNFHYLMQLNRLAGRRQGDPNYHPVLPWVVDFTTPHGRFRDLRKSKFRLNKGDKQLDFTYEMTRQAFVAGGAGGGEPPHVPHHISDVLSDITYYVYKARRTPRSVLCGHVRAQWEPHEYPASMERMQNWTPDECIPEFYTDPSIFRSIHPDMPDLDVPAWCSSSQEFVAAHRALLESREVSRDLHHWIDLTFGYKLQGKEAVKEKNVCLHLVDAHTHLASYGVVQLFDQPHPQRLAGAPALAPEPPLIPKLLVQTIQETTG"
  },
  {
    "reference": "MTKYVIHGGKPLFGEIDISGAKNAAVAIIPAALLVDGVCRIENLPQISDVTLLLNILQELGADVRTVNRTTVDVDCSHIRNARVPEELARKIRASYYLIGALLGRFGSAEVPPPGGCDFGGRPIDQHIKGFVAMGANVDVRGGYIHAKASNGRMNGTQIYLDIVSVGATMNIMLAAVLAAGMTIIENAAKEPHIVDLANFLNSMGAEIMGAGTDVIKIRGVERLTGGTYSIIPDQIEAGTYMTAVAATGGEVLIKNVIPKHLECITAKLLEMGVDVEEQDDAVLVRRTGKLTRTNVKTLPYPGFPTDMQPQIAAVLCLAEGTSVITEGVWDNRFRYVDEFRRMGAQIQVDGKIAVIEGVEKFTGARIQACDLRAGAAMVIAGLAAQGTTEIGHIHHIERGYEDIVRKLSGVGADIRVVVTPDEDKQAQVG",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. A protein that can perform UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity in membrane, cytoplasm to support cell wall organization, UDP-N-acetylgalactosamine biosynthetic process, cell division, peptidoglycan biosynthetic process.2. The protein should have a Helical domain that is long enough to span the entire membrane.3. The designed protein is the optimization of the Proton donor to achieve high catalytic efficiency for phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine =   phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine.4. For general function, the protein need meet that Adds enolpyruvyl to UDP-N- acetylglucosamine.; Cell wall formation5. The protein should be optimized for activity in peptidoglycan biosynthesis, Cell wall biogenesis pathway and exhibit minimal cross-reactivity with other metabolic pathways.6. The protein should have a high affinity for UDP-N-acetyl-alpha-D-glucosamine, phosphoenolpyruvate. The designed protein sequence is ",
    "response#1": "MTQNGGKALKGSIEINGAKNAAVAILPAAILASKGECIIDNVPDIADVHCLERIIRSLGCNVEKLDNNTLKINAEEIKTVEACGNDVRKMRASYYFIGALLARFKEAKVELPGGCPIGVRPIDQHIKGFEALGAKVSIEHGAVNIMAEKLIGTNIFFDVVSVGATINLMIAATLAEGTTVLENAAREPHVVDVANFLNAMGANVKGAGTDVIRIKGVKELKGCNYSVVPDQIEAGTFMIAAAATRGDVTIQNVIPKHLESISAKLLEMGAKVEEGDDSVRVYVDGDLKGVNLKTAPYPGFPTDVQQPMCTLLSTAKGRSIIVETIWENRHKHVDELKKMGATIKVEGRSAIIDGVDRLTGAVVKATDLRAGAAMVIAGLISDGVTEITSIEHIDRGYPHIEEKLNALGANIIRKGGKQLNGTWQVQGMKNAATPVLAATLLTREQCFIHNIPRISDISHMLEILEDLGSKVAWVDEHTVQIHTPEIKKHEMDYLLTKRMRSSVLCMGPLLASIGNVLMPEPGGCNIGNRPLDAHFKAFEALGAKVKFEQDKYYTINGKNLQAGKIKLIEKSVTATENAMMASSRLSGTTVITNCAQEPHIMCLADFLRAIGVKVDGAGTNEIRITGSKDLKGCEFEIIPDQLEVGTIAVLGALCGGEIEISPVVSEHMEVIKQKLDHAGVRVDEKAGTWIVRGSKNSLSAFEIKTEPYPGFPTDLQAPFGVLATQVKGNSVICDPMYENRLAYMNELNKMGAKTVIKDEHTAEVEGPTDLKGADIHSLDLRAGATLIIAALAAIGESVINEAEIIDRGYEKIDER",
    "response#2": "MTSPGGHPLRGHVKISGAKNSALVIMAGALLCSGDCRIRNVPLLADVERMGEVISALGVRLTRQADIIDINASEIKTSKAPYELVTQLRASFFAIGAILARLGVAQMPLPGGCAIGARPVDLHVRGLQAMGAEVQIEHGICNAYVPGSGGRLKGAKIYLDTPSVGATETLMMAATLADGETILENAAREPEVVDLANFCKAMGANIQGAGTSTITIVGVPKLHSVDYSIIPDRIEAGTFLVAGAITRSEITLSSVVPDHLIPLIAKLRDIGVTIIEESPDCLRILPAEILKATDIDTLPHPGFPTDMQAPFMALLTLAEGDSIINESVFENRLRHASELNRLGADIRVKGNTAFVRGVPILSGAPVIGTDLRASAALVIAGLAAEGKTTIQGLHHLDRGYDQIVTELGSEPGAVSALKGGKQLNGTWQVQGMKNAATPVLAATLLTREQCFIHNIPRISDISHMLEILEDLGSKVAWVDEHTVQIHTPEIKKHEMDYLLTKRMRSSVLCMGPLLASIGNVLMPEPGGCNIGNRPLDAHFKAFEALGAKVKFEQDKYYTINGKNLQAGKIKLIEKSVTATENAMMASSRLSGTTVITNCAQEPHIMCLADFLRAIGVKVDGAGTNEIRITGSKDLKGCEFEIIPDQLEVGTIAVLGALCGGEIEISPVVSEHMEVIKQKLDHAGVRVDEKAGTWIVRGSKNSLSAFEIKTEPYPGFPTDLQAPFGVLATQVKGNSVICDPMYENRLAYMNELNKMGAKTVIKDEHTAEVEGPTDLKGADIHSLDLRAGATLIIAALAAIGESVINEAEIIDRGYEKIDER",
    "response#3": "MTSSGGHQLNGTVRLEGAKNAVLPVLAASLIASEGESVIKEVPVLADVYTINEVLRNLNAEVEFDSTTKTVNINASQQLETEAPFEYVRKMRASVLVLGPLLARYGHAKVAMPGGCAIGSRPIDLHLKGFEAMGAEIHVGNGYVEANVNGRLQGAKIYLDMPSVGATENIMMAAALAEGKTVIENAAKEPEIVDLANYLNKMGANIVGAGTETIRIIGVEKLRGTEHMIIPDRIEAGTFMVASAITGGNVFIENAMREHLRSVISKLEEMNVDVIDENGGLRIIGPEKLKSTDIKTLPHPGFPTDMQSQMMSLMLRAEGTGVITETVFENRFMHVEEFRRMNANIKIEGRSVIIEGISELQGAEVAATDLRAAAALILAGLVSDGYTRVTELKHLDRGYVDIVDKLRLLGADITRVR"
  },
  {
    "reference": "MVSAAASAGRLGSALPFLLVLLDLQYQGAECGINAEVEKQLEMGKKLLAAGQLADALSHFHAAIEGDSDNYIAYYRRATVYLAMGKSKAAIRDLSKVVELKQDFTSRLQRGHLLLKQGKFDEAEDDFKNVLKSNPSNNEEKEAQTQLTKSDELQRLYSQALSAYRQEDYEAAIPLLDEILAVCVWDAELRELRAECYIKEGEPSKAISDLKAAAKLKSDNTEAFYKISRIYYQLGDHELSLSEVRECLKLDQDHKQCFSLYKQVKKLNKQIESAEEFIREGRYEDAISKYDSVMKTEPDVPVYATRAKERICHCLSKNQQATEAITVCTQVLQLEPTNVNALKDRAEAYLLEDLYEEAIKDYETAQANSENDQQIREGLERAQRMLKQSQKRDYYKILGVKRNARKQEIIKAYRKLASQWHPDNFQSEEEKKKAEKKFIDIAAAKEVLTDPEMRRKFDAGEDPLDAESQQGGGNPFHRNWNTWQGFNPFGSGGGPFTFKFHFS",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The presence of J domains is critical for the interaction of the designed protein with its intended targets.2. Incorporate a signal peptide in the protein design.3. For general function, the protein need meet that May be involved in the unfolded protein response (UPR) during ER stress.4. The ideal protein would be one that plays a critical role in response to unfolded protein, negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation, positive regulation of translation initiation in response to endoplasmic reticulum stress through protein kinase inhibitor activity, protein kinase binding within endoplasmic reticulum, cytosol. The designed protein sequence is ",
    "response#1": "FCPKDVLGHGAEGTIVYKGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLHQTTSGLAHLHSLNIVHRDLKPHNILLSMPNAHGRIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKDNPTYTVDIFSAGCVFYYCS",
    "response#2": "FCPKDVLGHGAEGTIVYKGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLHQTTSGLAHLHSLNIVHRDLKPHNILLSMPNAHGRIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKDNPTYTVDIFSAGCVFYYVI",
    "response#3": "FCPKDVLGHGAEGTIVYKGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLHQTTSGLAHLHSLNIVHRDLKPHNILLSMPNAHGRIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKDNPTYTVDIFSAGCVFYYVC"
  },
  {
    "reference": "MKHRIGRVETQIQREVDDILLKDVNDPRVKGVTITGVKLTGDLQHATIFYSILDDAPDKVEAAQTGLDKASGLIRREVGQRIRLFKVPEIEFAQDKSVQYGARIDQLINEVRRKNLE",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be expressed specifically in the cytoplasm The designed protein sequence is ",
    "response#1": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTCTV",
    "response#2": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC",
    "response#3": "GKGLYAKRDIAKGDLIWSEEPLFFIPPLANVNLMKTASACTYCGKLLQRTESATVLKGLDCNVCSEVWCSIKCKHLDGNLHSLLKHNLYNPGSKKHKLIDAEAFLELQDYCLEEQWNALYAITLIYANCITDKSGVKQKQFDAMARVSQDVRYKALNSSAGTFDSLNGGALFVQEQQEHLWKIGYEKFLRVFPKKPVEYREFLFMMGTYNINNLDSNVFLTQSHLNHNCASNTSVETELNRTAGLKVIAGRDIKSGEELTTTC"
  },
  {
    "reference": "MQLSAFLQGLIHEPNFLPNEADFDVKNLALDSRKITENSVFFALPGTQLDGRKYITDAIKNGAKAVLIEAENEDETVTFIEKIPLIPVTFLRHKMGVMAAKFYDFPAQKLRILGVTGTSGKTSCTHFFAQILQNLGQKCGVIGTLGSGFYGDLSDVSLTTPDAISLQAKLHLFVQEQAKAVSMEVSSHSIDQGRVNGVTFELGIFTNLTQDHLDYHGDMATYAAVKHRFLTHYPMNHLIMNIDDVHGRQWVQALADKRSVFAYSTQPVADLPGYIPHIYTDQIQLSLSGIRAHVYSPWGEGELQLPLIGQFNLSNALAVLTALCVYGVPFQTVLTEFAKLKSVPGRMQLIGEYHQPLVIVDYSHKPDSLANALQALRAHVAGQLICVFGCGGNRDAGKRPIMAKIAETFADTVIVTNDNPRHEDPEEIARQIMQGFERPDQVIVELDRAKAIQKSIQLATIDDCILVAGKGAEQYQQIGDEKIPFDDVTVAQSFLKTFSL",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. A protein with UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity is needed to enhance cell cycle, cell wall organization in a specific context.2. The protein contains novel Mur ligase N-terminal catalytic domains that confer a unique function or activity.3. The protein should have a high affinity for UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate.4. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "GRMQRLGGQGAPLVVIDYAHTPDALDKVLTSLRPAVAVGGELVCVFGCGGDRDRGKRSEMGRVAARLADRVVVTSDNPRSEEPGAITSDIVHGIRETGNRRYAVEIDRAAAIATAIAEARVGDVVLLAGKQV",
    "response#2": "VTGTNGKTTISQLVAQLAQLMGMSSAVMGTAGNGKLGALVQASHTTGDALAVQQFLYQMGQENADLLALEASSHGLDQQRLQGMPASVAIYTNLSRDHLDYHADMAEYAAAKAKLFDKTHFPELTHAIINLDDEHAQIMLDMAHTSALTVWTYSLNPTKNATFVAATINPSLQGVEIAVRYNVSNNTGDVHLDSTDANHDQTNTITVISPLLGRFNVANLLAAIAIMRAEGIPDVAIDAALATFHGVRGRMQQVQAGKGDVHAIVDYAHTPDALAKVLETLRTVRSAGPATGANGEARILCVVGCGGDRDRTKRPVMARTAAELADLVILTSDNPRSEDPSAILGEMREGLDPIQMRKVTYNTDRAEAIRSACMQAQAGDVILVAGKGHETVQIIGDRVLPFDDRQVAREALA",
    "response#3": "VTGTNGKTSCAHWIAAGLDASGRRAAVLGTLGNGLWGALEDAPNTTADAALLHETLRHLASIGAQAAAMEVSSHGLDQGRVNGVAFDVALFTNLSRDHLDYHGTMAAYGAAKAKLFAWPGLRFGVINADDPFGQSLIDAARSRGRKVLTYGFGAADIVGTHLAASSTGLAFAVETPWGKGEIHTRLVGAFNAANLLGVLGGRLQAIVNGAFHAYVDYAHTPDALENVLNALRLHTQGRLICVFGCGGDRDKNKRAVMGEIVARLADIAIVTDDNPRSENPAAIRKQVLSQMDGDVHEIADRGEAIKFAVQQMNENDILLVAGKL"
  },
  {
    "reference": "MSQASSSPGEGPSSEAAAISEAEAASGS",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein should be expressed specifically in the host cell nucleus The designed protein sequence is ",
    "response#1": "GTWNVRSMNPGKLDVVKQEMERINIDILGISELKWTGMGELNSDDHYIYYCGQQSLRRNGVALIVNKRVRNAIIGCNLKNDRMISVRFQGKPFNLTVIQVYAPTPYAEEGEVYRFYEDLQHLLEITPKIDVLFIIGDWNAKVGSQEIPGITGRFGLGMQNEAGRRLIEFCHHNRLVITNTLFQQPSRRLYTWTSPDGRYRDQIDYIICRQRWRSSVQSAKTRPGADCGSDC",
    "response#2": "GTWNVRSMNPGKLDVVKQEMERINIDILGISELKWTGMGELNSDDHYIYYCGQQSLRRNGVALIVNKRVRNAIIGCNLKNDRMISVRFQGKPFNLTVIQVYAPTPYAEEGEVYRFYEDLQHLLEITPKIDVLFIIGDWNAKVGSQEIPGITGRFGLGMQNEAGRRLIEFCHHNRLVITNTLFQQPSRRLYTWTSPDGRYRDQIDYIICRQRWRSSVQSAKTRPGADCGSDC",
    "response#3": "GTWNVRSMNPGKLDVVKQEMERINIDILGISELKWTGMGELNSDDHYIYYCGQQSLRRNGVALIVNKRVRNAIIGCNLKNDRMISVRFQGKPFNLTVIQVYAPTPYAEEGEVYRFYEDLQHLLEITPKIDVLFIIGDWNAKVGSQEIPGITGRFGLGMQNEAGRRLIEFCHHNRLVITNTLFQQPSRRLYTWTSPDGRYRDQIDYIICRQRWRSSVQSAKTRPGADCGSDG"
  },
  {
    "reference": "MGETTEGKVVAQNKKARHDYFIEETFEAGIVLTGTEIKSIRAGKMNLKDSFARIKNGEAFLYNAHISEYEQGNRYNHEPTRPRKLLLHKKQINTLIGQTQQKGYSIVPLKVYIKNGFAKVLIGLAKGKKNYDKRETLRRKDAKREVERAIKERMRG",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. For general function, the protein need meet that Required for rescue of stalled ribosomes mediated by trans- translation; tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame; Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes; The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation The designed protein sequence is ",
    "response#1": "ELLHL",
    "response#2": "MEEKRTREE",
    "response#3": "MNNERYTDER"
  },
  {
    "reference": "MPEDSPFPSASALSAAAIVVAAGQGLRAGQPLPKQFALWRGKPVVRHSVDALLAAGVKPIVVVIPAGAEALAARALNGVGDQVRVISGGVHRQDSVRAGLEALDGNAPEHVLIHDAARPQLPAQVIERLRQALAATPGAIPVLPVVDSLAHADGELMGAPARREALRRVQTPQAFRYGAILAAHRAWAGDTTAGDDAQVAQAAGLSVALVEGDEALHKLTFANDFEGDAPPLRVGSGYDVHRLEDGEALWLCGVRIEHHQGLSGHSDADVAIHALVDALLGALGEGDIGQHFPPSDPQWRGASSDRFLAHAAGLVAAAGYRLGNADLTIICEAPKIGPHRDAMRERLAAILGVDKRAVSVKATTTEKLGFTGRGEGIAAQAVVSLVRN",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The stability of the protein-a divalent metal cation complex should be optimized to maximize enzymatic activity.2. The designed protein should have a high specificity for a divalent metal cation, 4-CDP-2-C-methyl-D-erythritol 2-phosphate binding, with minimal off-target effects.3. The desired protein should be able to perform 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-   erythritol 2,4-cyclic diphosphate + CMP4. The protein design should take into account the physiological conditions of the isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6, Isoprenoid biosynthesis pathway.5. A protein with 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity functions is required to enable isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway, terpenoid biosynthetic process.6. The designed protein should contain one or more 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase domains that are essential for its function. The designed protein sequence is ",
    "response#1": "MRVGTGFDVHRLADGEDLWLCGVKIDHHQGLAGHSDADVGLHAVVDAILGACARGDIGDHFPPSDPQWKGAASSAFVQHAVKLAGEAGYAIGNVDLTLICEAPKIGPHRGAMAVRLAELLGIPVSAVSVKATTTEKLGFTGRGEGIAAQAVTTRVGTGFDVHRLADGEDLWLCGVKIDHHQGLAGHSDADVGLHAVVDAILGACARGDIGDHFPPSDPQWKGAASSAFVQHAVKLAGEAGYAIGNVDLTLICEAPKIGPHRGAMAVRLAELLGIPVSAVSVKATTTEKLGFTGRGEGIAAQAVTTGWLHSPAAPRVGTGFDVHRLVAGRPLFLCGVEVPHSMGLDGHSDADVGLHALCDAIYGALAEGDIGRHFPPSEMRWKDADSAAFLRHAAGRIAARGGMLANADVTLICERPKITPHAPAMIARVAELLGVPESRVSVKATTTERLGFPGREEGIAAQAAVSLLV",
    "response#2": "RTGMGFDVHRLAEGEELWLGGIRVQHDKGLLGHSDADVGLHALTDAILGAIAEGDIGTHFPPSDPQWRGAPSSRFLEHAAHLVAQRDGRIEHVDLTLICEAPKIGPYRDAIRARIADLLRLAPGRISVKATTTERLGFTGRGEGIAAQAVATVALVEGDKRLKKLTFAEDFVSQTPDVRTGMGFDVHRLVAGEELWLGGIRIEHDKGLSGHSDADVALHTIVDALLGAVARGDIGDHFPPSDPQWKGASSDKFLLHAVKLVGEAGYRIGNIDLTLICEEPKIGPHREAMRARIAELAGVDIDRISVKATTTERLGFPGRGEGIAAQAIATVIGTK",
    "response#3": "MRVGTGFDVHRLADGEDLWLCGVKIDHHQGLAGHSDADVGLHAVVDAILGACARGDIGDHFPPSDPQWKGAASSAFVQHAVKLAGEAGYAIGNVDLTLICEAPKIGPHRGAMAVRLAELLGIPVSAVSVKATTTEKLGFTGRGEGIAAQAVTTLVTTAEDAVAAADRFRTGMGFDVHRLAEGEELWLGGIRVQHDKGLLGHSDADVGLHALTDAILGAIAEGDIGTHFPPSDPQWRGAPSSRFLEHAAHLVAQRDGRIEHVDLTLICEAPKIGPYRDAIRARIADLLRLAPGRISVKATTTERLGFTGRGEGIAAQAVATTTRRLT"
  },
  {
    "reference": "MIRILVINPNSTVQMTESVKSVLDDCTPPNVQLEYLTCPPEGPKAIECVSDGVRSAAVLMKYFEDHPPQVDAFLVSCYSDHPLVTTLRETYRKPCTGIMQASILTALSLGRKVSVVTTTKRYEPLLTDGIHAMGISDSVFAGIASTGLAPLELDSKPRAEVDALLARTALRAVNEMGADVICLGCAGMTHMAHVLEKAVGPNIPIIDGTKAGVELLASLVRMNLFTSKQGVYQAVGSD",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. A protein that can perform racemase activity, acting on amino acids and derivatives in cytosol, nucleus to support nitrogen compound metabolic process. The designed protein sequence is ",
    "response#1": "LQYGFSAGQPELLNFIRDHTKIIHDLKYKDWDVLATAGNTNAWESTLRVFCNRGDVILVEAHSFSSSLASAEAQGVITFPVPIDADGIIPEKLAKVMENWTPGAPKPKLLYTIPTGQNPTGTSIADHRKEAIYKIAQKYDFLIVEDEPYYFLQMNPYIKDLKEREKAQSSPKQDHDEFLKSLANTFLSLDTEGRVIRMDSFSKVLAPGTRLGWITGSSKILKPYLSLHEMTIQAPAGFTQVLVNATLSRWGQKGYLDWLLGLRHEYTLKRDCAIDALYKYLPQSDAFVINPPIAGMFFTVNIDASVHPEFKTKYNSDPYQLEQSLYHKVVERGVLVVPGSWFKSEGETEPPQPAESKEVSNPNIIFFRGTYAAVSPEKLTEGLKRL",
    "response#2": "LQYGFSAGQPELLNFIRDHTKIIHDLKYKDWDVLATAGNTNAWESTLRVFCNRGDVILVEAHSFSSSLASAEAQGVITFPVPIDADGIIPEKLAKVMENWTPGAPKPKLLYTIPTGQNPTGTSIADHRKEAIYKIAQKYDFLIVEDEPYYFLQMNPYIKDLKEREKAQSSPKQDHDEFLKSLANTFLSLDTEGRVIRMDSFSKVLAPGTRLGWITGSSKILKPYLSLHEMTIQAPAGFTQVLVNATLSRWGQKGYLDWLLGLRHEYTLKRDCAIDALYKYLPQSDAFVINPPIAGMFFTVNIDASVHPEFKTKYNSDPYQLEQSLYHKVVERGVLVVPGSWFKSEGETEPPQPAESKEVSNPNIIFFRGTYAAVSPEKLTEGLKRI",
    "response#3": "LQYGFSAGQPELLNFIRDHTKIIHDLKYKDWDVLATAGNTNAWESTLRVFCNRGDVILVEAHSFSSSLASAEAQGVITFPVPIDADGIIPEKLAKVMENWTPGAPKPKLLYTIPTGQNPTGTSIADHRKEAIYKIAQKYDFLIVEDEPYYFLQMNPYIKDLKEREKAQSSPKQDHDEFLKSLANTFLSLDTEGRVIRMDSFSKVLAPGTRLGWITGSSKILKPYLSLHEMTIQAPAGFTQVLVNATLSRWGQKGYLDWLLGLRHEYTLKRDCAIDALYKYLPQSDAFVINPPIAGMFFTVNIDASVHPEFKTKYNSDPYQLEQSLYHKVVERGVLVVPGSWFKSEGETEPPQPAESKEVSNPNIIFFRGTYAAVSPEKLTEGLKRC"
  },
  {
    "reference": "MERTFIAIKPDGVQRGLVGTIIQRLESRGYQLVGLKLVQVSQELAEAHYAEHKERPFFHSLVKFITSGPVVAMVWQGKGVIAAARKLIGKTNPLEAEPGTIRGDFGIDIGRNLIHGSDGVETAQREIALWFDESELVSWTPVAQSWIYE",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein should be optimized for activity in Purine metabolism pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. The protein must exhibit the following characteristics: (Microbial infection) Catalyzes the phosphorylation of dZDP to dZTP, when the bacterium is infected by a phage that produces the substrate for the synthesis of dZTP (2- amino-2'-deoxyadenosine 5'- triphosphate), which is then used by the phage as a DNA polymerase substrate..3. The designed protein should have nucleoside diphosphate kinase activity, metal ion binding, ATP binding to facilitate CTP biosynthetic process, GTP biosynthetic process, UTP biosynthetic process.4. The designed protein must have a Pros-phosphohistidine intermediate active site that is highly conserved among related enzymes.5. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "MGTLTVITGPSGVGKGTLVQRLLARNPSIWVSVSATTRAPREGEREGESYFFHSRERFDALVQEGGLLEWAEFAGNCYGTPRAPVEQQLQAGRPVLLEIELEGARQVRRSFSKARQIFLAPPSFEELERRIRGRGTDSEDAIQQRLLRAREELSAQGEFDAVVVNDDLDQALLKLTRDP",
    "response#2": "MGTLTVITGPSGVGKGTLVQRLLARNPSIWVSVSATTRAPREGEREGESYFFHSRERFDALVQEGGLLEWAEFAGNCYGTPRAPVEQQLQAGRPVLLEIELEGARQVRRSFSKARQIFLAPPSFEELERRIRGRGTDSEDAIQQRLLRAREELSAQGEFDAVVVNDDLDQALLKLL",
    "response#3": "MGTLTVITGPSGVGKGTLVQRLLARNPSIWVSVSATTRAPREGEREGESYFFHSRERFDALVQEGGLLEWAEFAGNCYGTPRAPVEQQLQAGRPVLLEIELEGARQVRRSFSKARQIFLAPPSFEELERRIRGRGTDSEDAIQQRLLRAREELSAQGEFDAVVVNDDLDQALLKLQL"
  },
  {
    "reference": "MEPVDPRLEPWKHPGSRPKTACNNCYCKKCCWHCQVCXLKKALGISYGRKKRRQRRRTPHYSQIHQVSLSKQPASQPRGDQTGPKESKKKVERETKADPDD",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein must be optimized for actinin binding. The designed protein sequence is ",
    "response#1": "VGPGELILYPRRRKESHKGENGIVAIIGGGAYTGAPYLAGMAALRAGCDLVYVCCPSSVWSVVASFSPDLIVRKISGEFLTVEGLKEVEDIIEKADAIVVGPGLGKRKEVLEACKFIFENYDKKFVYDADAIEVLKGKKCNQKVIITPHSGEFKRLTGIDLPDDIEERSRIVKKEAKKLEATILAKGAIDIISDGKRLKMNKIHNEGMTVGGTGDTLAGICAAMLAKKIAPFYSACISAFINGMAGNMAYEEKGNIVATDMIEKIPLVIKKV",
    "response#2": "GPGELILYPRRRKESHKGENGIVAIIGGGAYTGAPYLAGMAALRAGCDLVYVCCPSSVWSVVASFSPDLIVRKISGEFLTVEGLKEVEDIIEKADAIVVGPGLGKRKEVLEACKFIFENYDKKFVYDADAIEVLKGKKCNQKVIITPHSGEFKRLTGIDLPDDIEERSRIVKKEAKKLEATILAKGAIDIISDGKRLKMNKIHNEGMTVGGTGDTLAGICAAMLAKKIAPFYSACISAFINGMAGNMAYEEKGNIVATDMIEKIPLVIR",
    "response#3": "VGPGELILYPRRRKESHKGENGIVAIIGGGAYTGAPYLAGMAALRAGCDLVYVCCPSSVWSVVASFSPDLIVRKISGEFLTVEGLKEVEDIIEKADAIVVGPGLGKRKEVLEACKFIFENYDKKFVYDADAIEVLKGKKCNQKVIITPHSGEFKRLTGIDLPDDIEERSRIVKKEAKKLEATILAKGAIDIISDGKRLKMNKIHNEGMTVGGTGDTLAGICAAMLAKKIAPFYSACISAFINGMAGNMAYEEKGNIVATDMIEKIPLVIKKFGS"
  },
  {
    "reference": "MKVTRHEKILELIERKDIETQEELAEELRKSGIEITQATVSRDIKELKLIKVLGEQGKYKYAAIVKNENDLSDKLANIFSHSVVSVENINNFVVVKTLSGSGNAAAEAIDSLNFKEIAGTIAGDNTIFIMARTSEQAFEIVKKMKRVIRERGV",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be optimized for activity in L-arginine biosynthesis [regulation] pathway and exhibit minimal cross-reactivity with other metabolic pathways.2. A protein that contributes to arginine biosynthetic process, protein complex oligomerization by functioning in cytoplasm with arginine binding, DNA-binding transcription factor activity. The designed protein sequence is ",
    "response#1": "DDLLVSVDHSGNIAVLRTPPAGAPFLASFIDRVGMDEVVGTIAGDDTVFVLARDPMTGKELGEFL",
    "response#2": "MKKAERQRLIKQLIMQQEIETQDELITRLEEIGVRATQATVSRDIREMSIVKTHGADGRVKYAIFK",
    "response#3": "DDLLVSVDHSGNIAVLRTPPAGAPFLASFIDRVGMDEVVGTIAGDDTVFVLARDPMTGKELGEFL"
  },
  {
    "reference": "MSLSTKRILLGISGGIAAYKSADLVRRLKERGADVRVVMTDSAKEFITPLTLQALSGNPVGDSLLDPSAEAAMGHIELARWADLILVAPATASLLARVSAGLADDLLTTLILASSAPLAVAPAMNQQMYKAVATQENLATLKRRGVHIWGPNGGEQACGDVGPGRMKEPLELVAEVEGLFGPKPLAGKRLVLTAGPTREPLDPVRYLSNHSSGKMGFAIAAAAAAMGAEVELITGPVALATPAGVKRTDVGSALEMHAAALELAGGCDIFIGCAAVADYRPVAANEHKIKKDGDTLSVEMVKNPDIISDVAAMAARPALVVGFAAETRDVEQYAKGKLARKGLDLIAANAVGNGLAFGTDDNQLTLISKDQTQVLPQASKAKLAEQLLAILANRLSEH",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. For general function, the protein need meet that Catalyzes two steps in the biosynthesis of coenzyme A; In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.3. The Proton donor of the protein must be engineered to enable effective catalysis of H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO2.4. The designed protein should have FMN binding, metal ion binding, phosphopantothenoylcysteine decarboxylase activity, phosphopantothenate--cysteine ligase activity to facilitate coenzyme A biosynthetic process, pantothenate catabolic process.5. The CTP binding site should be stable and able to withstand changes in environmental conditions.6. The protein design should consider the endogenous proteins and enzymes present in the coenzyme A biosynthesis, Cofactor biosynthesis, CoA from (R)- pantothenate: step 3/5 pathway to avoid interference with native metabolic processes.7. The protein contains novel DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains that confer a unique function or activity. The designed protein sequence is ",
    "response#1": "KKILFGMTGGISAYKVAGFISHLVQVGAEIQVIMTEAAKKFIGEITINALTRKPVYSNLFDYGEKILHIHLMREYDNLVVAPATANIIGKMAGGIGDDLLSSCFLVDPKKVILVPAMNVDMWNNPIVQENIKKLIAIGVRVMDPSSGNLACGERGTGRLPEQEELCAAIEVILNKKQDLQGKSVMITAGGTIERIDPVRYITNDSSGKMGFAIAEAARDYGAKVTLIAGQTQVEPPEGVEVIRVESTQDMYDAVMSRFDNMDIIVKAAAVSDYRPAVRADQKIKKTTDRMTIELVKNPDILQELGARKNGQFLIGFAAETHDVAAFAQDKLRRKQCDLLVANDVSRDDAGFGSNNNEVQFFYKDGRKEVFPLKSKTETAKNIVQFILEYI",
    "response#2": "SVLLIVGGGIAAYKALELIRRLREHGLRVRTVLTDSGARFVTPMSIQALSGERVFSDLFSLTDESEMGHIALSRSSDLIVVCPATADLLARMAAGLANDLATTVLLATDTPVLVAPAMNVRMWTHAATQANVATLHARGVAFVGPDSGPMACNESGPGRLAEPDDILSAILRLARAAVAGSGAAVLAGRKAVVTAGPTFEPIDPVRGLTNRSSGKQGYAIAAALVELGAEVTLVSGPTALGAPVGVTRIDAESALEMQAATQNALPADIAVLSAAVADWRVDTIAGGKIKKGPGGPPTLALIENPDILAGIAAPGPNRPALVIGFAAETTDLEANARAKLSRKGCDWIIGNDVSDDVFGSDGNAVLVCWAEGREELARGSKRALADELVALIAERLPAADS",
    "response#3": "KKLLLGVTGGVAAYKVAELARLLTQKGVVVQTVMTKSACRFVGPATFQSLTGLPVFTELWEANVASNMAHINLSRNVEMILIAPASADFIAKLACGLADDLLSTLCLARNCPMMIAPAMNRQMWENPATQRNINRLQDDGVIVAGPADGEQACGEVGPGRMLETLAGAIEGWFRPLSGKRVLISAGGTREFLDPVRFIGNPSTGKMGIALAQAALDRGADVTLVHAPLGILEPLPGNLEAIAVVSAAEMHEAMLQALPQADWIIMAAAVADVKPSHTSLEKLPKSALPDSLPLVPVPDIVTELARHKQPHQRLIGFAAQTGDIVTPAKDKLERKKLDAIAANAIDQPNSGFGSDMNQAVLTALAQTARIPLTGRKVLITAGGTREPLDPVRFIANRSSGKMGYALATEAVRRGGQVVLVSGPVELAPPPGLAEFIRVERAEEMAEAVLSRAPEMDVIIKAAAVADFRPVQVAEQKVKKGDGDWVLHLQKNPDILAILGARKRPGQVLVGFAAETHDLLANGAEKARRKGADFIVANDVSRPGSGFGSDTNEVTFLVSDGTEERIPRADKAELAHEILNVVADRLDRS"
  },
  {
    "reference": "MSFLSGKNVILGISGGIAAYKSPLIVRLLKKRNCNVRVVMTPSAKDFVSPLTLSTLSENDVLSTFTSDDENNKKWNNHVSVAKWADLFIIAPATSNTISSMVSAKCDNLLIATYLSCTVDVYIAPSMDLDMYENAANQNNIDTLIKLGKKVLPVGIGYLASGLEGKGRMLEAEEIIQYIENDLSKNLPLYGKKVLITAGPTYESIDPIRFIGNRSSGKMGFELAKRAAELGSHVFLFSGPTSLDINNSSIEIIRVNNSDEMLEKVMTRYDKMDIVISAAAISDFKSKKVLSNKIKRKEKSINLEFVPTKDILEHMGKRKKNQFLLGFALETENEIENSLLKLKNKNLDAIVLNLFNNDNLVFGSEDNKVTYIRSKNDITHFSMKSKRLVAVDIFNQILSEYA",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. For general function, the protein need meet that In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.; Catalyzes two steps in the biosynthesis of coenzyme A2. The protein design should consider the endogenous proteins and enzymes present in the CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis, coenzyme A biosynthesis pathway to avoid interference with native metabolic processes.3. The designed protein should contain one or more DNA/pantothenate metabolism flavoprotein C-terminal, Flavoprotein domains that are essential for its function.4. The CTP binding site should be stable and able to withstand changes in environmental conditions.5. The desired protein should be able to perform H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-   phosphopantetheine + CO26. The protein design must be able to perform metal ion binding, FMN binding, phosphopantothenate--cysteine ligase activity, phosphopantothenoylcysteine decarboxylase activity in order to contribute to pantothenate catabolic process, coenzyme A biosynthetic process.7. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "KKIVLGISGGIAAYKSPELVRRLRDRGAEVRVVMTHAAKAFITPLTLQAVSGYPVSDDLLDPAAEAAMGHIELAKWADLVIMAPATADLLARVATGMANDLLTTACLATAAPIAAVPAMNQQMYRAAATQANLQTLASRGMLLWGPDSGSQACGDVGPGRMLDPLEIVALAHDGRLAEIVEEKDASPEQLRIRLVNGGIYAFSAGLLRSVLPRLSADNAQGEYYLTDVLAIARAEGGRVAAVTTDDPWETDGVNSRAHLAALAAAARRRINEEWMLA",
    "response#2": "KKIVLGISGGIAAYKSPELVRRLRDRGAEVRVVMTHAAKAFITPLTLQAVSGYPVSDDLLDPAAEAAMGHIELAKWADLVIMAPATADLLARVATGMANDLLTTACLATAAPIAAVPAMNQQMYRAAATQANLQTLASRGMLLWGPDSGSQACGDVGPGRMLDPLEIVALAHDASGLSEPGRLVGRVLVAVTGGVAAIKAPHLLRLLKRAGHEVRVLATPRALEFVTPLSLAVAAGGEVATEEAWFRPDGRALHIDLARFADLVLVAPATADALAKAALGLGDDLLSATLLAGPRRVAWAPAMNEAMWLAPQTQGHVERLKALGHAFFGPGHGPLAAEGEGEGWGRMLEPEELLERVRAALAGEPRPLSGLKLLVSAGPTREYLDPVRFLSNPSSGRMGYALAEAARDRGAEVVLVSGPTCLQDPWGMEVVRVESALEMRAAILERYPHLQAVVMAAAVADYRPAEVHRDKEPKTGEEKTLRLLPNPDILKELGANKGNRVLVGFAMETREGLHRAKEKLLRKNLDLIALNYVGREGVGFGSEENEVVLLRPGREEPVAVAAGTKREIADRLWDIVAPLIAER",
    "response#3": "KKILIAVSGGIAAYKINLLIRDLVKKGAEVQILMTPSAEQFVSKLTLSVLSKNPVYSDFYDQNGTWNNHVEFALWADVIIMAPCTANTLGKMVHGICDNLVIATYMSARCPVFIVPAMDLEMYQHPSTKQNLEIAEDFGHFIIPAESGELASGLVGQGRMAEPETIARTIEDDGLGRPAGPLAGXRVLVTSGPTHEPIDPVRYIANRSSGAQGSAVADALLALGADVIFVTGPSNVEPPIGAIVIKVETAIQMLEAVEKEMPCDAAVFVAAVSDWRVENSQSSKIKKEKGQTPVAEFVENPDILAMISNRKKARPGLVVGFAAETEDVLENATKKLARKGCDWIVANDVSPESAIMGGSENAVTLEVFWPGGREELPRMPKRELAGRLVERIAEALA"
  },
  {
    "reference": "MTVPSALVPILLLGTAAVMVQCLPLSFQRNDTVERRWETLFSRSMGEKKDTSRDSDYLLGIKRQRRLYCNVGIGFHIQVLPDGRINGMHSENRYSLLELSPVEVGVVSLYGVKSGMFVAMNAKGKLYGSRYFNEECKFKETLLPNNYNAYESRKYPGMYIALGKNGRTKKGNRVSPTMTLTHFLPRI",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein must exhibit the following characteristics: Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. The designed protein sequence is ",
    "response#1": "GHGGLNQLGGAFVNGRPLPEVVRQRIVDLAHQGVRPCDISRQLRVSHGCVSKILGRYYETGSIRPGVIGGSKPKVATPKVVEKIGDYKRQNPTMFAWEIRDRLLAEGVCDNDTVPSVSSINRIIRTR",
    "response#2": "GHGGLNQLGGAFVNGRPLPEVVRQRIVDLAHQGVRPCDISRQLRVSHGCVSKILGRYYETGSIRPGVIGGSKPKVATPKVVEKIGDYKRQNPTMFAWEIRDRLLAEGVCDNDTVPSVSSINRIIF",
    "response#3": "GHGGLNQLGGAFVNGRPLPEVVRQRIVDLAHQGVRPCDISRQLRVSHGCVSKILGRYYETGSIRPGVIGGSKPKVATPKVVEKIGDYKRQNPTMFAWEIRDRLLAEGVCDNDTVPSVSSINRIIF"
  },
  {
    "reference": "MSKAYRVGILGATGAVGNELLELLESRNFPLADLKLLASERSVGKNLPFKGENIAIEAVNENSFKNIDILLASAGSSTSKAWAHKAVDAGAVVIDNSSAFRMNPEVPLIVPEVNPDAAANHKGIIANPNCTTILMAVAIYPLHKIKKIQRIVAATYQSASGAGAKAMAEVQAQASAILAGKKPPTEVFPYPLAFNLFPHNTPLNDMGYCEEEMKMVNETQKIFGTQNIRITATCVRVPVLRAHSEAINLEFAEPFSANEAREILKKAPGVKVVEDWQANYFPMPIDATGRDEVLVGRIRQDISHACGLELWLCGDQIRKGAALNAIQIAELLVEKNIVKPANALVSR",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. A protein that is capable of catalyzing reactions within the Amino-acid biosynthesis, L-threonine from L-aspartate: step 2/5 pathway with high efficiency and specificity.2. The Acyl-thioester intermediate active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The designed protein must possess protein dimerization activity, aspartate-semialdehyde dehydrogenase activity, NADP binding to support isoleucine biosynthetic process, 'de novo' L-methionine biosynthetic process, threonine biosynthetic process under certain conditions.4. The designed protein should contain one or more Semialdehyde dehydrogenase NAD-binding domains that are essential for its function.5. The phosphate, NADP(+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MELKPIYDEVGIHRINVATYQAVSGAGRRAIEELAGQTASLLNGKGAHPEVMPKQIAFNAIPHIDTFQDNGYTREEMKMVWETQKIFDDEALAVNPTTVRIPVFYGHSEAIHVETREKMTAERARDVLRGAPGVSVIDEREAGGYPTAVSDAADADPVXVGRIREDISCXNGLCLWVVADNIRDDIHALKPIYDAVGIKRINVATYQSVSGAGRSAVKELARQTSDLLNGRPLDIAGDAKQIAFNAVPHIDEFLDNRYTREEMKLVWETRKILDDDSIAINPTAVRIPVFYGHSEAVHIETETKISAGDVCELLRNAAGVELMDGTEAGQYPTAVTESSGNDPVYVGRVREDISHARGINLWIVSDNIRDNLHADEVTLSYLKPIYDEVGIHRINVATYQAVSGAGRRAIEELAGQTASLLNGKGAHPEVMPKQIAFNAIPHIDTFQDNGYTREEMKMVWETQKIFDDEALAVNPTTVRIPVFYGHSEAIHVETREKMTAERARDVLRGAPGVSVIDEREAGGYPTAVSDAADADPVXVGRIREDISCXNGLCLWVVADNIRDDLHADEVTLSYALKPIYDEVGIHRINVATYQAVSGAGRRAIEELAGQTASLLNGKGAHPEVMPKQIAFNAIPHIDTFQDNGYTREEMKMVWETQKIFDDEALAVNPTTVRIPVFYGHSEAIHVETREKMTAERARDVLRGAPGVSVIDEREAGGYPTAVSDAADADPVXVGRIREDISCXNGLCLWVVADNIR",
    "response#2": "KIALVGATGMVGEEMIKVLEEFNFPCSEFIPSASERSVGKELTFKGKTCKVVGLKEAVALQPDIVLFSAGASTSLEWAGEFAKNGSFVIDNSSAWRLYEDVPLVVPEVNGHTLHDDLNVMKRENIRVNATCVRVPVFVGHLAPLHKRYGIDKVVVSTYQAVSGSGHKAIVELENQMKDANYPNNVYPKRIATNCIPHIDVFNDNGFTKEELKMVYETQKILEDNKIKVNSTTVRIPVFFSHSESVYVEFKETPEVSEVKQILSEAPGVIVLDNPEKNEYPTPLEVANSDDTYVGRIRKDLFNPNALSMWVVADNLRNITPELNKLLKPLHDYAKIKRVVVSTYQAVSGAGKAGTDSLTNEAKSYLETGKVQTTGKFQYQIAFNCIPHIDVFQEGGNTKEENKMIFETCKIMGDDSIKVSATCVRVPVWYGHSESVNIETERPLSPEKARGLLATAPGIKVVDEPAKNGYPMPLFSNDEDLTMVGRIRKDESIANGLNMWVVSNNVRAKVTDALNATRAAVEEGIVPGGGVALLYANKALDKLTLNNHDVQAGINIVLKALEAPLKQIAANAGYEGSVVIDKVKNSNVGVGYDALNNKYVDMFKEGIVDPTKVVRTALQDASSVASLLITTEAMIAEKPEKNNAPMPGGDMGGMGGMGGMY",
    "response#3": "LVGLVGWRGMVGSVLMQRMQEEGDFSHIEPVFFSTSNTGGKAPAMAKNETTLKDANDIEALKKCEIIITCQGGDYTSEIFPKLRAAGWNGYWIDAASTLRMKEDAIIVLDPVNLNVIKDALAKGGKNWVGGNCTVSLMLMALKPLHDLFKIKRIVVSTYQAVSGAGKDGMDELFAQTKGTFVNDPPTIAQFTKQIAFNVIPHIDRFMEDGSTKEEWKMAVETRKILDPDIQVMATCVRVPVFIGHSEAISIECEKPVDLKKARNALRKALGVILRDEREDGGYVTPIETVGEDATYVSRLRIDPSVENGLALWCVSDNLRWGAAEPLRRMLRILEQ"
  },
  {
    "reference": "MDLVFASNNQNKIIEIQNLVPKNFKILSLSDIGCYEDIPETGSTIEENAYLKAIYIAKKYNLNCFADDSGLEIEALHGEPGVFSARYAGIPKNDQNNIDKVLQKLENIPNRSANFKTVICLNIDNQMHYFEGKVFGTIISEMRGTNGFGYDPIFVPDNYNKTFAEMSLEEKSKISHRAIAVQKLTTFLQK",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.2. For general function, the protein need meet that Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP3. The designed protein should have a high specificity for substrate binding, with minimal off-target effects.4. The protein should have a fast turnover rate for dITP + H2O = dIMP + diphosphate + H(+).5. A protein with metal ion binding, ITP diphosphatase activity, XTP diphosphatase activity is needed to enhance nucleotide metabolic process in a specific context. The designed protein sequence is ",
    "response#1": "MRIILLGAPGAGKGTQAQFIMENYGIPQISTGDMLRAAIKEGTELGKKAAEVMDAGALVSDELIIGLVKERIAQEDCKKGA",
    "response#2": "MSILSGKVALVTGSGRGIGAATAKKLGAAGAKVAVNYAGSAEGAEEVVQAI",
    "response#3": "MSIILGIDPGSRITGFGVIRQQGRQLTYVGSGCIRTSSADLPLRLKQVFDGLSEIIRQYQPDEFAIERVFMAKNADSALKLGQARGAAIVAAVNQELPVFEYAARQIKQTVV"
  },
  {
    "reference": "MTLNLADLRQRYLKDGLDENNTDDNPFVQFEKWFHQAQKSELLEPNAMVLSTVNEVNQPSTRTVLLKQFSDDGFVFFTNYRSQKAKDIQHNPKVALHFNWLELERQVKIQGVAAKISLKDSMRYFATRPKGSQIGAWVSHQSEVISSKQLLLSQFEKMKQKFQSGEIPFPDFWGGYMVVPQQIEFWQGGDNRLHDRICYTLKDNQWVKQRLAP",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein requires FMN to activate a critical catalytic site.2. The protein must exhibit pyridoxamine-phosphate oxidase activity.3. The protein design should consider the endogenous proteins and enzymes present in the Cofactor metabolism, pyridoxal 5'-phosphate salvage pathway to avoid interference with native metabolic processes.4. The desired protein should be able to perform O2 + pyridoxine 5'-phosphate = H2O2 + pyridoxal 5'-phosphate The designed protein sequence is ",
    "response#1": "LNEAIEQNVPEPYAISLATCGSDNMPAVRTVLVREVTELGVVFYTNYLSHKGQDIEQNPHAEALFFWHDMQRQVRVRGVIAKVDRAKTEAYFQKRPYESQVGAWVSQPQSGEVASREL",
    "response#2": "LNEAIEQNVPEPYAISLATCGSDNMPAVRTVLVREVTELGVVFYTNYLSHKGQDIEQNPHAEALFFWHDMQRQVRVRGVIAKVDRAKTEAYFQKRPYESQVGAWVSQPQSGEVASREL",
    "response#3": "MEFWQGGEHRMHDG"
  },
  {
    "reference": "MGVTAVLMLPLLLLGISGLLFIYQEVSRLWSKSVVQNKVVVITDAISGLGKECARVFHTGGARLVLCGKNWERLQSLYDALISVADPSKTFTPKLVLLDLSDISCVQDVAKEVLDCYGCVDILINNASVKVKGPAHKISLELDKKIMDANYFGPIILTKALLPDMISRRTGQIVLVNNIQGKLGIPFRTAYAASKHAALGFFDCLRAEVEEYDVVVSTVSPTFIRSYHVDPGQGNWEASIWKFFFRKLTYGTHPVDVAEEVMRTVRRKKQEVFLANPIPKAAVYIRTLFPELFFAVVACGVKEKLSVPEEG",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. For general function, the protein need meet that NADH-dependent oxidoreductase which catalyzes the oxidation of all-trans-retinol to all-trans-retinal2. Incorporate a signal peptide in the protein design. The designed protein sequence is ",
    "response#1": "PPMVDTVQGKVLGKYISLEGFTQPVAVFLGVPFAKPPLGSLRFAPPQPAEPWSSVKNATSYPPMCFQDPVTGQIVNDLLTNRKEKIPLQFSEDCLYLNIYTPADLTKSDRLPVMVWIHGGGLVLGGASTYDGLVLSTHENVVVVVIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALHWVQDNIAKFGGDPGSVTIFGESAGGESVSVLVLSPLAKNLFQRAISESGVALTAGLVKKNTRPLAEKIAVISGCKNTTSAAMVHCLRQKTEEELLGTTLKLNLFKLDLHGDSRQSHPFVPTVLDGVLLPKMPEEILAEKNFNTVPYIVGINKQEFGWILPTMMNYPPSDVKLDQMTAMSLLKKSSFLLNLPEDAIAVAIEKYLRDKDYTGRNKDQLLELIGDVVFGVPSVIVSRGHRDAGAPTYMYEFQYSPSFSSEMKPDTVVGDHGDEIYSVFGAPILRGGTSEEEINLSKMMMKFWANFARNGNPNGQGLPHWPEYDQKEGYLQIGATTQQAQKLKEKEVAFCRR",
    "response#2": "PPMVDTVQGKVLGKYISLEGFTQPVAVFLGVPFAKPPLGSLRFAPPQPAEPWSSVKNATSYPPMCFQDPVTGQIVNDLLTNRKEKIPLQFSEDCLYLNIYTPADLTKSDRLPVMVWIHGGGLVLGGASTYDGLVLSTHENVVVVVIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALHWVQDNIAKFGGDPGSVTIFGESAGGESVSVLVLSPLAKNLFQRAISESGVALTAGLVKKNTRPLAEKIAVISGCKNTTSAAMVHCLRQKTEEELLGTTLKLNLFKLDLHGDSRQSHPFVPTVLDGVLLPKMPEEILAEKNFNTVPYIVGINKQEFGWILPTMMNYPPSDVKLDQMTAMSLLKKSSFLLNLPEDAIAVAIEKYLRDKDYTGRNKDQLLELIGDVVFGVPSVIVSRGHRDAGAPTYMYEFQYSPSFSSEMKPDTVVGDHGDEIYSVFGAPILRGGTSEEEINLSKMMMKFWANFARNGNPNGQGLPHWPEYDQKEGYLQIGATTQQAQKLKEKEVAFY",
    "response#3": "PPMVDTVQGKVLGKYISLEGFTQPVAVFLGVPFAKPPLGSLRFAPPQPAEPWSSVKNATSYPPMCFQDPVTGQIVNDLLTNRKEKIPLQFSEDCLYLNIYTPADLTKSDRLPVMVWIHGGGLVLGGASTYDGLVLSTHENVVVVVIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALHWVQDNIAKFGGDPGSVTIFGESAGGESVSVLVLSPLAKNLFQRAISESGVALTAGLVKKNTRPLAEKIAVISGCKNTTSAAMVHCLRQKTEEELLGTTLKLNLFKLDLHGDSRQSHPFVPTVLDGVLLPKMPEEILAEKNFNTVPYIVGINKQEFGWILPTMMNYPPSDVKLDQMTAMSLLKKSSFLLNLPEDAIAVAIEKYLRDKDYTGRNKDQLLELIGDVVFGVPSVIVSRGHRDAGAPTYMYEFQYSPSFSSEMKPDTVVGDHGDEIYSVFGAPILRGGTSEEEINLSKMMMKFWANFARNGNPNGQGLPHWPEYDQKEGYLQIGATTQQAQKLKEKEVAFNL"
  },
  {
    "reference": "MSSLSVYHVSSPEIPNKVLTHFEDIVATLAEQGVRFDRWEAAVKIQPGARDEEIIAAYQARIDGLMTERGYVAVDIVSVSSDHPEQAGELDEYRHSQDDVRFFVTGRGLFNLHLGDYVYAVLCERNDLITVPAGMPHWFDGGERPHFVSIRLSGDSHGVVAELTGDDIARRFPRLED",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should consider the endogenous proteins and enzymes present in the L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6, L-methionine biosynthesis via salvage pathway pathway to avoid interference with native metabolic processes.2. The protein should be designed to catalyze the following reaction 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-   methylsulfanyl-2-oxobutanoate + formate + 2 H(+).3. The protein must be able to acireductone dioxygenase [iron(II)-requiring] activity, acireductone dioxygenase (Ni2+-requiring) activity, iron ion binding.4. The protein must be engineered to have a cooperative binding mechanism with Ni(2+) to ensure proper regulation.5. The protein must exhibit the following characteristics: Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway..6. The Fe(2+), Ni(2+) binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "MSTAVDPLQVAVDARDAAALRRYRFPHADGRAVLYFTGNSLGLQPRAARDAVAAELDAWAELAVEGHFYAEKPWWDYHERLAKPLSAVVGALPIEVVVMNTLTVNLHLLMVSFYRPTATRFKIICEEKAFPSDQYMFQSQVRFHGLDPDDAIVEFRRRPGEETLRTEDIEAAIAAHGDTLALVLMGGVNYYTGQLFDIPAITAAAHAAGAIAGWDLAHAAGNVPLRLHDWDADFAAWCSYKYMNSGPGNASGCFVHERHLGRSDIPRFEGWWGTDKATRFRMAPGFEPIAGADAWQVSNTAVLAMSAHRAALEVFEEAGMAALRTKSELLTGFLEGLLAEVDDAELFEVITPRDPAERGCQLSLLLHGQGRALFERLAAEGVVADWREPNVIRLAPAPFYCSFADMRRFGEILRRGLAAR",
    "response#2": "MSQSPRSDITLGDLFTLYGITPAXGXDAXVXAXATXDAXDXDXIWFPXDXAXGXEAXXIIXXLXAQXCXSXVIXEXGXTNXXTVXSTXKXDXT",
    "response#3": "MLKGKTLKEALESIEQGELLLVIDDEDRENEGDLIIAAEAVTPEKVNFMSKYGRGLICTPMEDKRCKELDLPLMVEENTSSFGTAFTVSIDAKEGVTTGISSKDRALTIRAVIDPSAKPEDFRRPGHMFPLVAKEGGVLKRAGQTEGSVDLARLAGLYPAGVICEVMNEDGTMARLPDLEQFAKKHNLKIISIQDLIEYRMRTEKLVKQEM"
  },
  {
    "reference": "MTDQHAFATEQVQLDPTLSPTTEETTHFGFKTVAKSEKQKMVANVFHSVAGKYDLMNDLLSFGIHRVWKRFTIDCSGVRKGHKVLDLAGGTGDFTAKFSRLVGPSGEVILADINDSMLRVGREKLRNLGVVGNVNYVQANAEALPFPDNTFDCVIISFGLRNVTDKDKALRSMFRVLKPGGRLLVLEFSKPILDPLSKIYNFYSFNILPKIGEVVVNDSESYRYLAESIRMHPAQDVLKQMMIDAGFEQVNYYNLSAGIVALHRGYKF",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein design should take into account the physiological conditions of the Cofactor biosynthesis, ubiquinone biosynthesis pathway.2. The S-adenosyl-L-methionine binding site should be located in a region of the protein that is accessible to the ligand.3. The designed protein should have S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity to facilitate methylation. The designed protein sequence is ",
    "response#1": "LPLL",
    "response#2": "GRI",
    "response#3": "MEIILANPRGFCAGVDRAISIVERALELHGAPIYVRHEVVHNRFVVDGLRDRGAIFVEELDEVPDDAVLIYSAHGVSQAVRNEARSRGLRVFDATCPLVTKVHMEVARASRKGEESILIGHAGHPEVEGTMGQYASPEGAIYLVETAEDVANLKVTNESKLSFMTQTTLSVDDTSEVIDALKARFPHIVGPRKDDICYATQNRQDAVKDLAAECDVMLVVGSPNSSNSNRLREVGEKVGCKSYLVDNADKIEASWFDGVAKVGVTAGASAPEVLVQQVIEKLKALGAEAPEQLTGREENIVFEVPKELRV"
  },
  {
    "reference": "MSKQIVLASGNAKKVAELQSLLGELHMTVTPQTELGVSDAIEVGTTFVENAITKARHAAVKTGLPAIADDSGISVAALDGRPGVYSARFAGEHATDQQNLELLLSELNGVPRAQRQATFWCVLVYMRHADDPTPLICSGSWTGEITTSAQGTQGFGYDPIFWVEEAGKTAAELSSAEKNHLSHRGKALRQLQALLLEDLKLEDLKLEDKSQQDLTQQAPLQTPFKESN",
    "instruction": "Design a protein sequence that exhibits the desired activity and specificity. 1. The protein should have a high affinity for Mg(2+).2. The designed protein must be able to perform ribonucleoside triphosphate phosphatase activity under specific conditions.3. The protein must exhibit the following characteristics: Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.; Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP.4. The presence of Mg(2+) is necessary for the protein to function in the desired environment. The designed protein sequence is ",
    "response#1": "MTMKTLVFATNNKGKLAEVRALL",
    "response#2": "MKRIVLASGNRGKVREIGMLLADLQLELIPQSEFDVPEAPEPHPTFVENALAKARHASRATGLPALADDSGLEVDALRGAPGVFSARYAGEPKSDARNNEKLLRELADVPDAQRSARFHCVLAFMRHADDPTPLICEGRWEGRILHEARGEGGFGYDPYFFLPELSQTAAELDPALKNRLSHRAKALARLRELL",
    "response#3": "MFLIVGLGNPGPKXDXAX"
  },
  {
    "reference": "MSELGPLYTAAEMRAAEAAYGRPTIELMERAGRAATEGLLRRYPHASRISVWCGAGANGGDGLVVARLLHAAGRQAELFLAGPEDKLAGDAATNLRLAREAGVPFADSPSEADVVVDALFGTGFSGPPRREAAARIEALSALGAPVVSLDVPSGVDASTGQVPGAAVRADLTITFHGTKVGLAVAPGRFHAGEVEVAEIGLAHSETRHQRVRPEILSLVPRRAARDNKYSAGFVVLVGGSPGFTGAPSLAAEAAMRAGAGYVRACVPESVASVLAQRFVEVTLTPVASDADGLVDAEAADAILAAAERADAVAIGPGLGRSEGVRRLVHRLLSELELPVVLDGDGLWALEGHLDWVFSRDAPTVLTPHSGELARLLGRDSDWVDANRLDAAGGAADDVGAIVCLKGADTLVAAPGRGLLICDLGNPGLASAGTGDVLTGVVAAFLAKGMDARLGAAAAAATAGLAARRASERHGFAGLLARDVVEALSPLLSAG",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The designed protein should be optimized for metal ion binding, ATP binding, NADHX epimerase activity, ADP-dependent NAD(P)H-hydrate dehydratase activity activity in order to improve nicotinamide nucleotide metabolic process.2. For general function, the protein need meet that Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration; This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.3. The protein should have a high affinity for (6S)-NADPHX.4. The designed protein must have at least one YjeF N-terminal domain.5. The protein design should incorporate a binding pocket that specifically recognizes Mg(2+) to enhance enzymatic efficiency. The designed protein sequence is ",
    "response#1": "MKEIDSYAIHTIGIPSMVLMERAALCVADEMGRAFPAASGRELCRAKTAVFCGTGNNGADGAAIARILHLRGCSVFIVTVGDRSRWTPELRSQIAIARNLGIPVRRFEEPGERTDSFDVAVDALFGVGLSREVEGSFREAIRFFDSVRPMFTVAVDIPSGIHSGTGAVLGAAVRADLTVTFGCEKAGTALYPGRGFAGRICVADIGFPPYDRPTAHKGEFGRVLVVGGGPYTGAPALAAQAALRAGADLAYVACPETVAGGVQGYSENLIVRPQAGDLLAPSSVPELLETASGMDAVVFGPGLGDADASLEAATAFLEAFDGTAVVDADALQVVPDVDTEATLVCTPHQGELEAMGGPAAVDTDERAALVADFAADLGVTLLVKGAVDVISDGERTRVNRTGNPGMTVGGTGDVLAGVTGAMTCPLEPIHAAALGAYANGRAGDLAVEEYGHGLVATDLLGAVVDLARRRSPEGHKGTYGHVLLAAGSLRMSGAGLLSARAALRAGCGLVTWALPEKLLPYVIGSVPELMLAPVTGGDGEWNAGTAAEVLRLSTSRDCVAIGPGLGRFEGDTEWLRRLWEESDRAMVIDADALNILADAGYRSWSRRHPVILTPHPGEMARLAGISTAEVQRDRIGLALSYAVQHGVILVLKGAHTVIATPEGQAYINITGHPGMGTGGAGDVLTGIISGLLAQGLEADQAAAFGVYLHGLAGERAARKRDHPAALIAGDIISA",
    "response#2": "MKEIDSYAIHTIGIPSMVLMERAALCVADEMGRAFPAASGRELCRAKTAVFCGTGNNGADGAAIARILHLRGCSVFIVTVGDRSRWTPELRSQIAIARNLGIPVRRFEEPGERTDSFDVAVDALFGVGLSREVEGSFREAIRFFDSVRPMFTVAVDIPSGIHSGTGAVLGAAVRADLTVTFGCEKAGTALYPGRGFAGRICVADIGFPPYDRLLVIGGSKNYSGAPAYSSLTGIGFGIDLVITYTPEVIGDVLRSYSPNLIVRSSPGDWLNLDCFDEVSWLVDWANTIVIGPGLGQEKETENLLVELLKLFRKEEKNFVLDADALKLVKNHLELLSDQSVILTPHEGELKIMTDIELPSFDNLDERIKIISDISQNLNVTLLIKGPYDYISDTRNIKINKTGCPEMAIGGTGDVLAGLCGCFLSIGNSTFNSACSAAFLNGINGEYCKKTIGPRFTAMDMIKNVVVIGGTRGYTGAAALASNAALRGGAGLVTAVIPEQLYPVAAVKLTEAMTYPASGSKGGGFSSTCRASIQQVLEKATVLAVGPGFGQEEETAGFLEDLLLNTSIPLVLDADALNILTRRKHLLTDASLAEQRRQWVLTPHPGEMARLCGVSISEIQADRLGYAAQASREWGVVVVLKGARTIITGPEGPLFINPTGNPGLASGGTGDVLAGLIASFRAQGLAAEEAAYSGAFIHGLAADQIALKKGTIGMIAGDLLKEIL",
    "response#3": "MRALDMNAEYFGISRLQLMENAGRGVAEEIASRFNPSDTRVTVFCGLGGNGGDGFVAARHLACMGFKVNVILAGKQSDIVNEEARKNWEALKSLRRSLSLQEVYDSSLIPEVKADVIIDALLGIGLKSPPRPPISQMIRRINEADAFRVAVDVPTGLDSDTGAVLGEAVKAHLTITFHKPKPGLLRAKDYVGELIVKHIGIPEEVEGPGDVLIVVKPRPPEAHKGDFGRLLVVGGSETFSGAPTLTALAALRTGVDLVYIAAPRETAYAISSISPDLITLKLEGDHLSMRNMPVIRSYMDRVTAVAIGPGLGLHKETEEAVREIVGIAEERRIPILLDADGLKAFANFKHRLATPAVLTPHLGEYMILTGERPPEDLEGRAEHIRRTAEGLNAVILLKSHVDIISDGFRVKFNFTGNPGMTVGGTGDVLSGIVAALLSQGIDPFEAAVAGAFINGAAGDFVANEKGYHMVASDLIEWIPKVMNDE"
  },
  {
    "reference": "MNDEIDKNQSESTSESENPAIPAPTQVTNRRPQSNRDWWPNQVDLSVLNKPSRDSDPLGEDFDYKAEFAKLDVEAVKRDVVEVMRTSQDWWPADWGHYGPLFIRMSWHAAGTYRIADGRGGGGQGAQRFAPLNSWPDNASLDKARRLLLPVKQKYGRKLSWADLLILAGNVAHDDMGLPTFGFAFGREDTWQPEEVFWGPEDTWLGDERYADEAPLELEGALSNVTMGLIYVNPEGPKGQPDPMGSAHDIKVTFRRMGMTDEETVALIAGGHTFGKAHGAGDPSLLGPEPEGCPVHSIGLGWQNPNGTGKGADAITSGLEGAWTPTPTTWDNSYWETLFTWDWELVESPAGAKQWQPKNPEAQELVPDAHIEGKKNPPMMSTADMALRVDPELRAYAERFRDDPEYFKDQYARAWFKLLHRDMGPKSRYLGPDVPSEDLIWQDPVPPVDHQLVGESEVADLKQRLLASGLTVSQLVHTAWSAAASYRGTDKRGGANGARLRLEPQIGWAVNEGVRDVLPVLERVKGEFEQQTGKKVSLADLIVLGGSAAVEKAAADAGVPITVPFHPGRTDASQEQTDVENFGWLEPQADGFRNWIAPGSKLEPETLLVDRAYMLELTAKEMTVLVGGLRMLGANTGGVRHGVFTDRTGVLSQDFFRNLLDLGITWSTSVDTEGVYEGRDAGGNVVRTATAADLVFGSNSILRGIVEVYSADDAKEKFVQDFVTAWVKVMELDRYDLR",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein should exhibit Polar residues compositional bias. The designed protein sequence is ",
    "response#1": "VIFTARFEGEPRSVTNDIHKKILLVKKLAYALGDRNSSTVSLRNITKGSIVVEWTNTSLPQHPCPKEQLTVMSRTLANADGRPSQTFRYSMEPEFRPLDVMVKGRASCRTYSFIPPGEIDIPEPPAVTPALGTGRQSTDDVYLHTVIC",
    "response#2": "VIFTARFEGEPRSVTNDIHKKILLVKKLAYALGDRNSSTVSLRNITKGSIVVEWTNTSLPQHPCPKEQLTVMSRTLANADGRPSQTFRYSMEPEFRPLDVMVKGRASCRTYSFIPPGEIDIPEPPAVTPALGTGRQSTDDVYLHTVIK",
    "response#3": "KTPVIFTARFEGEPRSVTNDIHKKILLVKKLAYALGDRNSSTVSLRNITKGSIVVEWTNTSLPQHPCPKEQLTVMSRTLANADGRPSQTFRYSMEPEFRPLDVMVKGRAR"
  },
  {
    "reference": "YAPSALVLTVGKGVSAATVTPERAVTLTCAPGPSGTHPAADSACADLAAVGGDLDALTRSEGVMCPMIYDPVLLTVDGVWQGERVSYERVFSNECEMNAHGSSVLAF",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The ideal protein would be one that plays a critical role in negative regulation of peptidase activity through serine-type endopeptidase inhibitor activity within extracellular region.2. The protein must exhibit the following characteristics: Strong inhibitory activity toward subtilisin.. The designed protein sequence is ",
    "response#1": "FDMYSKLVQLESREKNNILFSPYSVSAAMAICYEGTEGTAKEQISNVFYFPANNTVFKVRLKETNDKVYSGSDGYELENANAIWVQEEYPVKEEYIFNVKNYYRSEVTNLDFVEKPDESRDAINEWVEDKTDRKIKDIVPKGSITDDTRLILTNAIYFNGKWEHEFDKKMTSKKTFYPTKREEVSVDMMYISSNFNYSENLKAKIIELPYKGNDLSMYIVLPKGNNITEFENNFTVNEYTELKYNMESLGDVETSIPKFKFETKTELSDSLIEMGVADAFDLKLANFSGISEIRLLISRMIHQDFIDVQEEVTEAAASTTVFIGSVSSSKSKSREFKADLPFIFFIEDRRTDCILFMGKVEYP",
    "response#2": "MTAFALRLAKRLADVGVSSNKNLVFSPASLYAALALVAAGARGTTLDELLALLGAASLDDLEESVRRAVEVGLADESASGGPRVSDACGVWHDETLELKPAYRAAAAGTYKAVTRAANFQRQPKRSRKKINKWVSKATNKLIPEILPDGSVHVDTALVLVNAIYFKGKWSNPFPRSSTTTGKFHRLDGSSVDVPFMSSREDQYIGFHDGFTVLKLPYHHR",
    "response#3": "FDMYSKLVQLESREKNNILFSPYSVSAAMAICYEGTEGTAKEQISNVFYFPANNTVFKVRLKETNDKVYSGSDGYELENANAIWVQEEYPVKEEYIFNVKNYYRSEVTNLDFVEKPDESRDAINEWVEDKTDRKIKDIVPKGSITDDTRLILTNAIYFNGKWEHEFDKKMTSKKTFYPTKREEVSVDMMYISSNFNYSENLKAKIIELPYKGNDLSMYIVLPKGNNITEFENNFTVNEYTELKYNMESLGDVETSIPKFKFETKTELSDSLIEMGVADAFDLKLANFSGISEIRLLISRMIHQDFIDVQEEVTEAAASTTVFIGSVSSSKSKSREFKADLPFIFFIEDRRTDCILFMGKVEYPS"
  },
  {
    "reference": "MMADNEMSVVILAAGKGTRMYSDLPKVLHQLAGKPMVQHVIDAAKGLGAANIHLVYGHGGELLQSALKDPSLHWVEQPQQLGTGHAMQQAAPWFSDDEDILMLYGDVPLISQQTLQRLRDNKPEGGIGLLTVVLENPFGYGRIVRENGEVTGIIEQKDASEAQLTINEINTGILIANGKDLKRWLSQLTNNNAQKEYYITDIIAMAHQEGRRIEAVHPHSSSETEGVNNRQQLAALERVYQREQACKLLLAGVMLRDPERFDLRGELEHGRDVDIDINVILEGKVVLGNGVRIGAGCIIRNSVIGEGCEISPYSVIEDAHLEQACTVGPFARLRPGTVLADAVHVGNFVEIKKATLGKGSKAGHLSYLGDATIGAGVNIGAGTITCNYDGKNKSQTIIGDNVFVGSDSQFIAPVSVADNVTIAAGTTVMHNVDEESLVYNRKQQTEKTGWSRPVKK",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein should be able to bind Mg(2+) ligand in a variety of conditions2. A protein that can modulate the overall flux of metabolites through the Nucleotide-sugar biosynthesis, UDP-N-acetyl-alpha-D-glucosamine from N- acetyl-alpha-D-glucosamine 1-phosphate: step 1/1 pathway, enhancing or suppressing its activity as needed.3. The protein with the reaction acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-   acetyl-alpha-D-glucosamine 1-phosphate, the Proton acceptor must be optimized to ensure efficient catalysis.4. A protein that can perform UDP-N-acetylglucosamine diphosphorylase activity, magnesium ion binding, glucosamine-1-phosphate N-acetyltransferase activity in cytoplasm to support cell morphogenesis, cell wall organization, UDP-N-acetylglucosamine biosynthetic process. The designed protein sequence is ",
    "response#1": "ALILAAGKGTRMKSSMPKVLHKVGGVPMVEQVLRAVKAAGTQRQVVVVGFGGEAVQEYLGDRAETVVQEQQLGTGHAVMQAEPLLSGLSGTLLVTCGDTPLVTEDTFRSLLAHHEATQAAATVLTAVMPDPTGYGRVIRNEKGQVVKIVEQKDGSPEELAVREVNAGIYCFDMELLWQMLHNVTNNNAQGEYYLTDIIGMLVDAGKVVSAFAAPDFSETLGVNDRVALAEAEKILRARINRKHMLDGVTLINPEHTYIGPDVHIESDVTIEPGCQITGETTIKTGSHIGSNSEITDSIIGENSLVKQSVVTNSQVGREVNIGPFAHIRPESEVGDQVKVGNFVEVKKSQLGTNSKVSHLSYIGDADVGKDANIGCGTITVNYDGKNKYKTIIEDEAFIGCNSNLVAPVTVKKGAYVAAGSTITKDVPEKALSIARSRQENKEGYVDK",
    "response#2": "MSVIVLAAGHGTRMKSTTSKLLHKVAGKSMLSYAVQAAKALSPQHLVVVVGHGREQVQAHLGEIAPEATIAVQEEQLGTGHAVRCGLGALPPQVRGTVVVTYADVPLLSGETLGELVRTHARQANAVTVLTAELDDPTGYGRIVRDHGRVLRIVEHKDATPAEREIGEVNSGIYVFDSELLREGLAQIGRDNSQRELYLTDVVEYANRIGRGVGAFQTTDAWQTMGEDFALTTINSREQLAEVERMMQRRLNAAHMRDGLSVADAAAAEAARIASDAAVAAGSDAAAAVAASAAAAAAADDFMAQGVTLRDPQSVFFSWDTTIGRDVVIEPNVVFGPGVSVADGAVIRAFSHIEGATIGEGCEIGPFARLRPEAVLAEGVKVGNFVEVKKANIGKGAKLNHLSYVGDAEIGAKANLGAGTITCNYDGVNKDITVIGEGAFIGSNSSLVAPVTIGAGAYVGSGSVITRNVEPDALALGRARQVNKPGWVKRKRRAAAKAAAEAEAES",
    "response#3": "VVIMAAGLGTRMKSATPKVLHEIGGRSLVGHAVVTARALSPEHLVVVVGNGRELVEEHLKAVDPEARTAVQEQQLGTGDAVRAGLTAVPADFDGSVIVTSGDVPLLEAETLHDLIAEHDKHGNAVTVLTALREGYGRVVRDGDRIVAIREERDCDAAQRRNRRINAGIYCFNWPALRGGLPRLSRNNAQGEYYLTDVVALAVAEGAPVVAHPVDEAACLGINTRAQLAEAEAVLRQRIREHWMAQGVTLVDPRRIDVRGKLACGRDVLIDINAVFEGEVELGDGVQIGANAVIRDTSIGAGTVIHPHCVLEQSRIGANCQIGPYARTRPGVEMADDAKLGNFVEIKKSRIGKGSKINHLSYIGDAIIGANVNVGAGTITCNYDGANKHRTTIGDGAFIGSGVELVAPVEIGAGATVGAGSTITKAVPADELVLERSQQVIKQGWAKPRRQK"
  },
  {
    "reference": "MSRKVFLVGDGAVGSTFANDLLQNTTVDELAIFDVAKDRPVGDSMDLEDITPFTGQTNIHPAEYSDAKDADVCVITAGVPRKPGETRLDLVNKNVKILKTIVDPVVESGFKGVFVVSANPVDILTTLTQKISGFPKDRVIGTGTSLDSMRLRVELAKKLNVPVAKVNSMVLGEHGDTSFENFDESTVDNKPLRDYSEINDNVLSEIESDVRKKGGKIITNKGATFYGVAMMLTQIVSAILDNRSICLPLSAPINGEYGIKHDLYLGTPTIINGNGIEKVIETKLSDVEKAKMINSADKMQEVLSGVEM",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. Ideal protein should have a binding affinity for key enzymes in the (S)-lactate from pyruvate: step 1/1, Fermentation pathway to enhance the overall efficiency of the pathway.2. The protein must exhibit the following characteristics: Catalyzes the conversion of lactate to pyruvate..3. The designed protein must possess L-lactate dehydrogenase activity to support glycolytic process under certain conditions.4. The NAD(+), substrate binding site should be stable and able to withstand changes in environmental conditions. The designed protein sequence is ",
    "response#1": "LDSGRFSHFLAEEFGVSVNSVLGGHGDNMVPVLEYSTVSGIPVSELIEMGFSTKEKVDEIIKRTRGGGGEIVALLKTGSAYYAPATSGIAMAEAYLYDQKRILPAAAHLSGEYGIDNLYVGVPVVIGANGVEKVVEVKLSDEAKANLQVSVDAVKAA",
    "response#2": "LDSGRFSHFLAEEFGVSVNSVLGGHGDNMVPVLEYSTVSGIPVSELIEMGFSTKEKVDEIIKRTRGGGGEIVALLKTGSAYYAPATSGIAMAEAYLYDQKRILPAAAHLSGEYGIDNLYVGVPVVIGANGVEKVVEVKLSDEAKANLQVSVDAVKLARL",
    "response#3": "LDSGRFSHFLAEEFGVSVNSVLGGHGDNMVPVLEYSTVSGIPVSELIEMGFSTKEKVDEIIKRTRGGGGEIVALLKTGSAYYAPATSGIAMAEAYLYDQKRILPAAAHLSGEYGIDNLYVGVPVVIGANGVEKVVEVKLSDEAKANLQVSVDAVKR"
  },
  {
    "reference": "MTDAATLSTAGFDLTPPTEAERERLEANLTAEEARVLLHHGTEAPFCGGLLGEKSPGVYGCRLCGLPLFKHETKFESGTGWPSFYAPFAEDHVVGVRDTSYGMVRIETRCARCDSHQGHVFPDGPAPTRLRYCINSVSLQFVKAGAPLPDPLHRGDKIT",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein must have a Nucleophile active site that is highly conserved among related enzymes.2. The protein design must be able to perform zinc ion binding in order to contribute to protein repair. The designed protein sequence is ",
    "response#1": "LKKIHREQIMVLIGETGSGKSTQLVQFLADSGVAASESIVCTQPRKIAAMTLADRVREESSGCYEENTVSCTPTFSSTEEISSKVVYMTDNCLLQHYMKDRSLSGISCVIIDEAHERSLNTDLLLALLKKLLSRRIDLRLVIMSATADAKQLSQYFFSCGILLVF",
    "response#2": "LLDAYKSLIEAIMHAGIQTKTKVDIKFLDAEDIEANGTGSLADVQAILVPGGFGQRGFEGKIKAAQYARENRVPYFGICYGLHASIIDCARNVIGLEGAHSTEINPNTPYPVISLISEWTEKDGQVEQRDGDEDLGGTMRLGEQRCELVPGTLASSLYGKAEVYERHRHRWEVNHKYVAQFEAHGLRVAGRSDDGLVEIIELEGHPWHVGCQFHPEFTSSPRDGHPLFRGFIGMM",
    "response#3": "LLDAYKSLIEAIMHAGIQTKTKVDIKFLDAEDIEANGTGSLADVQAILVPGGFGQRGFEGKIKAAQYARENRVPYFGICYGLHASIIDCARNVIGLEGAHSTEINPNTPYPVISLISEWTEKDGQVEQRDGDEDLGGTMRLGEQRCELVPGTLASSLYGKAEVYERHRHRWEVNHKYVAQFEAHGLRVAGRSDDGLVEIIELEGHPWHVGCQFHPEFTSSPRDGHPLFRGFIRLYDTMTGAVRDFVPLRDGHVSIYLCGATVQGLPHIGHVRSGVAFDVLRRWLTAKGLDVAFIRNVTDIDDKILNKAADAGRPWWEWAATYERAFSAAYDALGVLPPSAEPRATGHITQMVELIERLIDRGHAYTGDGDVYFNVATLPDYGKLSGHRIDDVHQGEGVATGKRDQRDFTLWKGAKPGEPSWPTPWGRGRPGWHTECVAMCEAYLGAEFDIHAGGMDLVFPHHENEIAQAEAAGDGFARFWLHNGWVTMGGEKMSKSLGNVLSIPAVLQRVRAAELRYYLGSAHYRSMLEFS"
  },
  {
    "reference": "MTKYIFITGGVVSSLGKGIAASSLAAILESRGLKVTMTKLDPYINLDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFLKTTMSKKNNFTTGQIYDAVLRSERRGDYLGATVQVIPHITDEIKRRVYASAEGVDIALIEVGGTVGDIESLPFLEAIRQMRFELGSERSLFIHLTLVPYIRSAGEIKTKPTQHSVKELRTIGIQPDILMCRSELPIPVSDRRKIALFTNVEEHAVISAIDADTIYRIPILLHEQGLDDIVVRRLRLDVPPADLTEWHQVVQALKESHQEVRIAIVGKYVEHPDAYKSLNEALVHAGIRTKLKVKIVYIDAEVIEEEGAARLKDVDAILVPGGFGERGVEGKIATVRYAREHKVPYLGICLGMQSAVIEFARNVAQLEGAHSTEFLPNSPHPVIGLITEWLGESGQIETRDEHSDLGGSMRLGSQQCRLLQDSLAFSLYQKDVITERHRHRYEFNNQYIEKLEAHGLRFSGKSIDGRLVEVVEIPEHPWFLACQFHPEFTSTPRKGHPLFSGFVKAAFENRR",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The designed protein must be optimized for metal ion binding.2. A protein that can modulate the overall flux of metabolites through the CTP from UDP: step 2/2, CTP biosynthesis via de novo pathway pathway, enhancing or suppressing its activity as needed.3. The Nucleophile; for glutamine hydrolysis active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate. The designed protein sequence is ",
    "response#1": "LQDAYKSIGEAFIHTGSEHDCDVQVKWVDAEEVEKKGGDEMLKDFTGILIPGGFGIRGSEGKIEAARYARENNIPFFGICLGLQMAVAEFARNVAGLKNANSTEFKPRTRHPVIALMPGQRALTQKGGTMRLGSWACHLKRKTKAFDAYGKELIYERHRHRYEFNNKYKEPLTKAGMIFSGVNPDEGLVEMIELPDHPWFVGCQFHPELKSRLTNPHPLFLNFIEQAIERAGTKAGNKGAEAALEVKIAVVGKYVGLQDAYKSIGEAFIHTGSEHDCDVQVKWVDAEEVEKKGGDEMLKDFTGILIPGGFGIRGSEGKIEAARYARENNIPFFGICLGLQMAVAEFARNVAGLKNANSTEFKPRTRHPVIALMPGQRALTQKGGTMRLGSWACHLKRKTKAFDAYGKELIYERHRHRYEFNNKYKEPLTKAGMIFSGVNPDEGLVEMIELPDHPWFVGCQFHPELKSRLTNPHPLFLNFIKK",
    "response#2": "EDAYKSIAEALTHGGIANRVKVRAEWIDSEIFEREDPTPWLEGFHGILVPGGFGERGTEGKIRAAQFARERSVPYLGICLGMQMAVIEAARNLAGLSTAGSEEFDHEAGHKRFEPVVYHLKEWVEGNRTIKREVTDQKGGTMRLGAYNATLGDGSKVADIYGTRAISERHRHRYEVDIKYREKLEASGLKFCGMSPDGRLPEIVEFEGHPWFIGVQFHPELKSKPFEPHPLFADFVEDAYKSIAEALTHGGIANRVKVRAEWIDSEIFEREDPTPWLEGFHGILVPGGFGERGTEGKIRAAQFARERSVPYLGICLGMQMAVIEAARNLAGLSTAGSEEFDHEAGHKRFEPVVYHLKEWVEGNRTIKREVTDQKGGTMRLGAYNATLGDGSKVADIYGTRAISERHRHRYEVDIKYREKLEASGLKFCGMSPDGRLPEIVEFEGHPWFIGVQFHPELKSKPFEPHPLFADFVDAAQWVGLPDAYLSVAEALRAGGFAHDARVNIRWVTSDDCATPEGAARELDGVDAVCVPGGFGVRGIEGKIGAVRHSREHRVPLLGLCLGLQCMVIEYARHVAGLSGANSSEFDPDTAYPVIATMADQHDVVAGHRDMGGTMRLGLYPAELADGSLVRRLYAAAHVEERHRHRYEVANAYRNELTAAGLVFSGASPDGRLVEFVELPTAVHPFFVGTQAHPELRSRPIRAHPLFAGLVRD",
    "response#3": "DAYKSIYESLDHAGFQHSTRIIAKRIEAEDLERQGAEALLKGVDGVLIPGGFGMRGVEGKIQAARYARENNVPYFGICLGMQVAVIEFARNVMGLQDANSSEFDGDTQHPVICLLEEQVGMVEKGGTMRLGSQPCVLAEGSNSLQAYGTVEINERHRHRYEFNPQYREQYIHAGMAPVGTSPDGGLVEIVEVTDHPWYVAVQYHPEFKSQPNKPHPLFTGFS"
  },
  {
    "reference": "MRVTGTQMTWLNLWRWGTLILGLGIICNANDLWVTVYYGVPVWRDADTTLFCASDAKAHATEAHNVWATHACVPTDPNPQEIFMENVTENFNMWENKMVEQMQEDVISLWDQSLKPCVKLTPLCVTLTCSNNVTVNSNRTMTQGNFSGIIGNITDEVRNCSFNITTEIRDKKQKVHALFYKLDIVPINNNNNNSEYILINCNTSVIKQACPKISFDPIPIHYCAPAGYAILKCNDVKFNGTGPCSNVSSVQCTHGIKPVVSTQLLLNGSLAKGEIMIRSENITNNAKTIIVHLNKSVEINCTRPSNNTRASVRIGPGQVFYRTGDIIGDIRQAYCEINRTQWSEVLKQVKGKLKELFKNKNISFNQPSGGDLEITTHHFNCRGEFFYCNTSRLFNDTLLVNGTCKEREGCSIILPCRIKQIINMWQETGQAMYAPPISGTINCVSNITGILLTRDGGNDTSNNNTETFRPGGGNIKDNWRSELYKYKVVEIEPLGIAPTKAKRRVVQREKRAAGIGAMLFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQRFLGLWGCSGKIICTTAVPWNSSWSNKSYEQIWDNMTWAEWDREISNYTGQIYQILTESQNQQDKNEKDLLELDKWTSLWNWFDITNWLWYIKIFIMIIGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTPFHHQREPDRPEEIEEGGGEQDRGRSVRLVSGFLALAWDDLRSLCLYSY",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.2. The designed protein should have at least Helical transmembrane domains to ensure proper localization.3. The protein contains novel Human immunodeficiency virus 1 envelope glycoprotein Gp120 domains that confer a unique function or activity.4. The protein design should emphasize Basic and acidic residues compositional bias.5. The protein's functional design necessitates the inclusion of a Di-leucine internalization motif, YXXL motif; contains endocytosis signal motif.6. The protein must exhibit the following characteristics: The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Transmembrane protein gp41: Acts as a class I viral fusion protein; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.; During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.7. Include a signal peptide as an essential component of the protein. The designed protein sequence is ",
    "response#1": "MRVKGIRKNYQHLWKWGTMLLGLLMICSATEQLWVTVYYGVPVWKEATTTLFCASDAKAYDTEAHNVWATHACVPTDPNPQEIKLENVTENFNMWKNNMVDQMHEDVISLWDQSLKPCVKLTPLCVTLNCTDPLKNATNTNSTVNNNATVNNSTDEIKNCTFNITTEIRDKKRQVHALFYRLDIVPLSDEPNNSRDYRLINCNTSAITQACPKVSFDPIPIHYCAPAGYAILKCNDKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENIADNAKTIIVQLNKSVEINCTRPNNNTRKSIRIGPGQTFYATGDIIGDIRQAHCNISKAEWEETLQKVRNKLAKHFPNKTISFTRHSGGDLEITTHSFNCGGEFFYCNTSSLFNRTYPANSTGYNNETGNSTITIPCRIKQIINRWQEVGQAMYAPPIAGIITCRSNITGLLLTRDGGNTTNKTEIFRPEGGNMRDNWRSELYKYKVVEIKPLGIAPTKAKRRVVEREKRAIGIGALFLGFLGAAGSTMGAASLTLTVQARQLLSGIVQQQNNLLKAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNSSWSKKNVDYIWDNMTWMQWENEIDNYTGIIYTLLEESQNQQEKNEQELLELDKWASLWSWFDISSWLWYIRIFIMIVGGLIGLRIIFAVISIVKRVRQGYSPLSLQTHIPSPEEPDRPG",
    "response#2": "MRVRGMQRNWQHLRVWATIIVLLGILMICNATLWVTVYYGVPVWRDADTTLFCASDAKAHDTEMHNIWATHACVPTDPNPQEIRLENVTENFNMWKNNMVEQMQEDVISLWDQGLKPCVKLTPLCVTLNCTNANLTNVSLTTNNTFPSFNITGNITEEVRNCSFNMTTELRDKQQKVYALFYKLDIVPIDRDKNNSSNSEYRLINCNTSVIKQACPKVSFDPIPIHYCTPAGYAILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKTIIVHLNESVKINCIRPSNNTRTSIPIGPGQVFYRTGDIIGDIRKAYCKVNGTEWYKVLTGVTGKLKEHFPEKNISFQPPSGGDPEITMHHFNCRGEFFYCNTTNLFNNSCTNGTKRGCNETIILPCRIKQIINMWQGVGQVMYAPPIRGLINCTSNITGILLTRDGGNDTGNETFRPGGGNIKDNWRNELYKYKVVQIEPLGIAPTRAKRRVVQREKRAVGMGAVLLGFLGAAGSTMGAAALTLTVQARQLLSGIVQQQNNLLRAIEAQHHLLQLTVWGVKQLQARILAVERYLQDQQLLGIWGCSGKLICTTAVPWNTSWSNKSLGDIWDNMTWMEWDKEISNYTXLIYTLIEQSQNXQEKNEQDLLALDQWSSLWSWFXISXWLWYIRIFIMIVGGLIGLRIVFAVLSIVNRVRQGYSPISLQTHFPTQRGPD",
    "response#3": "MRVMGIQRNCPPWWIWGILGFWMLMICNGEDNLWVTVYYGVPVWKEANTTLFCASDAKAYDTEAHNVWATHACVPTDPNPQEVVLANVTEYFNMWKSNMVEQMHEDIISLWDQSLKPCVKLTPFCVTLNCSNATYTNSSENAKATPTPKTIVDPEMKNCSFNITTSMRDKMTKEQALFYALDVVPMDNDNDTIRDNGTASYRLRSCNTSVVTQACPKITFEPIPIHYCAPAGFALLKCNNKTFSGKGPCTNVSTVQCTHGIKPVVSTQLLLSGSLAEGEVILRSDNFTNNAKTIIVQLSTSVMIKCSRPSNNTSKSIHMGPGRAFYATERVIGNIRQAHCNLTRADWNRTLEKVVKKLGEQFENKTRISFNQPAGGDPEIVMHSFNCEGEFFYCNTTQLFNSTWVRDDANSSWRLNSSEGNDTLIILQCRIKQIINMWQEVGRAMYAPPIRGNISCLSNITGLILTRDGGIQNESQSQNGSESEETFRPVGGDMRDNWRSELYKYKVVKIEPLGVAPTRAKRRVVAREKRAVGLGAVFLGFLGAAGSTMGAASITLTVQVRQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERFLKDQQLLGIWGCSGKLICPTNVPWNTSWSNKTYNEIWDNMTWIEWEREINNYTQQIYSLIEESQNQQEKNEQDLLALDKWASLWNWFDISNWLWYIRIFIMIVGGLIGLRIVFAVLSIVKRVRQGYSPLSLQT"
  },
  {
    "reference": "MLYLEDYLEMIEQLPMDLRDRFTEMREMDLQVQNAMDQLEQRVNEFFMNAKKNKPEWREEQMTSIKKDYYKALEDADEKVQLANQIYDLVDRHLRKLDQELAKFKMELEADNAGITEILERRSLELDTPSQPVNNHHAHSHTPVEKRKHNPSSHHGATDHVPEKKFKSEALLSTLTSDASKENTPGRRNNNSSSSSNNAYNTNSSQPLASYNLGSLSSGSGAGAITMAAAQAVQATAQMKEGRRTSSLKASYEAFKNNDFQLGREFSLSRDSTGYSSSALASTLTQTLSSSSTDSRSGRKSKNNNKSSSQQSSSSSSSSSLSSCSSSSALAQELSQQTAVIPESDSNSQVDWTYDPNEPRYCICNQVSYGEMVGCDNQDCPIEWFHYGCVGLTEAPKGKWYCPQCTAAMKRRGSRHK",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. The protein should have a high affinity for Zn(2+).2. The designed protein should have a specific function within the Swr1 complex. The designed protein sequence is ",
    "response#1": "RNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPSSPATKNPCNIHYSPNDEDKGMVLPGTKCADGKACSNGRCVDC",
    "response#2": "PTTTIGSFPQVAGVRSARRSFKQGRISEEQYRAEMQRYIREAIAQQEALGLDVLVHGEPERNDMVEYFGEQLAGFCFTENGWVQGYGSRCVKPPIIYGDVERLKPMTMEWTTYAQQQTNRPLKGMLTGPVTMLCWSFVRDDQPRSATCKQIALAIRDEVQDLEQAGIKIIQVDEAALREGLPLRKSAWREYLGWAVDAFRLTASGVADSTQIHTHMCYSEFNHIAEWIAKMDADVISIEASRSNMELLGVLESFTYPNDIGPGVYDIHSPRVPSVAEMVELLRKASEVIPLERLWVNPDCGLKTRAWPETLASLRNMVAAAG",
    "response#3": "RNGQPCQNNNGYCYNGTCPIMRDQCIALFGPNAAVSQDACFQFNLQGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPSSPATKNPCNIHYSPNDEDKGMVLPGTKCADGKACSNGRCVDC"
  },
  {
    "reference": "MAGRVGQMKNQDEVHQNQILRELFLKELRAQKLYTQYHVNPLRKVHTITRKPMSWHDNLEEPEDAKFLNLIHHAAQGPKKKYSETQTEAQEIGWDPNPLINPDRQDHRLNHFRVYHDITLYKAKLWSLGEDDHQK",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. The designed protein should be expressed specifically in the ciliary base, centrosome, cytoplasm The designed protein sequence is ",
    "response#1": "DLNVDPSAPEPTGKVTFCINDRPQRVVMWLNQNFLLPEGIDSPDVTFSALRGGGLLRISLQTSGEITLRTDDIDLAGDLVQSLASFL",
    "response#2": "SLVLCDFTADGKNELLVGSEDFDIRVFKEDELVTEMAENETVTSLCHMHGSRFGYALANGTVGVYDRTARYWRIKSKNHAMSIHAFDLNADGVVELITGWSNGKIDAC",
    "response#3": "DLNVDPSAPEPTGKVTFCINDRPQRVVMWLNQNFLLPEGIDSPDVTFSALRGGGLLRISLQTSGEITLRTDDIDLAGDLVQSLASFL"
  },
  {
    "reference": "MPHGTAHVVHRRSPRRRRYPGGMSDSTPVRTTTPRQVRPRTEGWTQKVDAEGKPLLQFAAPKKGMPPAHLADMTPEQRVEKVKSLGLPAFRAGQLEKHYFQHYTSDPAQMTDLPAYGREELVHEMLPNLLTEVRRLETDRGDTIKFLWKLHDGALVESVLMRYPGRITLCVSSQAGCGMNCPFCATGQAGLTRNMSAAEIVEQVVRANRLIAEGGLGDPRRVGHDDERVTNIVFMGMGEPLANYNRVMQAVRTMVDKKHGLGMSARGITVSTVGLVPAINKLAAEDIPVTFALSLHAPDDHLRDEMIPINSRWKVDEALDAARGYFDKTGRRVSIEYALIKDMNDHAWRADLLASKLNERGRGWVHVNPIPLNPTPGSVWTASEPAVQNEFVRRLNEAGIPTTLRDTRGKEIDGACGQLVATEEDRDVAATVPVLD",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.2. The presence of Radical SAM core domains is critical for the interaction of the designed protein with its intended targets.3. The protein design should emphasize Basic residues, Polar residues compositional bias.4. A protein that contributes to rRNA base methylation by functioning in cytoplasm with tRNA (adenine-C2-)-methyltransferase activity, 4 iron, 4 sulfur cluster binding, tRNA binding, metal ion binding, rRNA (adenine-C2-)-methyltransferase activity.5. The presence of [4Fe-4S] cluster is crucial for the protein to function optimally under specific conditions.6. The designed protein is the optimization of the Proton acceptor to achieve high catalytic efficiency for adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-   adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-   deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-   adenosyl-L-homocysteine.7. The designed protein should have a binding site that is specific to the target [4Fe-4S] cluster, S-adenosyl-L-methionine ligand. The designed protein sequence is ",
    "response#1": "MTPEQIAEAMVAIGEPAFRGRQIAHWIYPKHAAAFAEMSDLPAELRTRLAESFSLLGARKKPPPHLADLDPAGRKALLEDNGLPGFRAKQLSTHYFARLVDAPEQMTDLPAAQRGELVSTLLPELMTPVRTSEADRGTTRKTLWRLFDGALVESVLMRYTDRTTMCVSSQAGCGMACPFCATGQGGLQRNMSTAEIVEQVMAGARSLARGEVPGGPGRVNNVVFMGMGEPLANYKAVVGAVRRLTDPAPDGLGMSARGVTVSTVGLVPRIRQLTEEGVPLTLALSLHAPDDELRNELVPINTRFSVAETVDAAWQYARTTKRRVSIEYAMMRGINDQGWRADLLADVLYATGADWGWVHVNLIPLNPTPGSKWTASDPADEQEFVRRLEARGIPTTVRDTRGREIDGACGQLAASGFREEIDAS",
    "response#2": "MTPEQIAEAMVAIGEPAFRGRQIAHWIYPKHAAAFAEMSDLPAELRTRLAESFGIGSIERTLCVSTQVGCAYGCKFCASGLDGFTRNLTAGEIVEQILQAEAISHERVDNLVFMGMGEPLANFENLMQAIRILNNQAWGVGIGARHITISTSGLVPRIRELADQPLQVRLAISLHGATDEVRGKIMPVNQRYNVAALMEACQYWHERKKQRLTFEYILIAGVNDGDAQAERLAKLAVPLKAKINLIPYNTVTGLNWQRPAIDRQDAFHKILRHPAISDVSDAALLAWLRKRGEPAYRAKQIRRHATHGTAAEFAELTDLPKQLREALAASFSWTSVDPVREDASADGETRKALLRLADGHHIETVLMPHHGARNAVCVSTQAGCPMACAFCATGEMGLVRNLTAGEIVDQVRHWQRELAQRGERVSHVVFMGMGEPFNNYDATLASTRALIDPEAFGISPRRLTISTCGVVTGIDQLAGEGLPINLAVSLHAPDDPTRARIMPINRKWGVEEVLAAAARYVKRTKRRVTFEYVLLAGVNDSEETARELAALITRHGPTKDAHVNLIPYNPGAGGFRRPSVERMERFAALLQEHGIAATLRISKGQDIAAGCGQLKVAEEGTR",
    "response#3": "MTPEQIAEAMVAIGEPAFRGRQIAHWIYPKHAAAFAEMSDLPAELRTRLAESFEIVRHEVARQQVSSDGTIKFLFELADGNAVETVLMRDGGRHTACVSTQVGCGMGCVFCASGLNGVARNLTPGEIVEQLVLLRNLTAPDRRLTHVVVMGMGEPLANLENLLDALAVAGDKNGLGIGARHVTISTVGLPPKIRRLADLGKQYHLAISLHAPNNTLRTEIVPTNNKTGIPEILAAADYFFEKTGRQVTFEYVVLGGLNDKPIHARQLQGLLCGRKAHVNLIPWNDVEGLPYKRPRDTDLKSFIEELRRNGVSVTVRHRKGSDIDAACGQLRARSTQVGCPVGCAFCATGLEGFRRNLSVAEIVGQVLDITYYVRQEDPDFQVTNIVFMGMGEPLLNYDSVLKSIQLLNDAQGLNIGMRRMTISTSGVVPKIIQLAEDNPQVGLAISLHAARDEIRNSLVPMNRRYPLNQLMRACQEYTHIHPAISDVSDAALLAWLRKRGEPAYRAKQIRRHATHGTAAEFAELTDLPKQLREALAASFSWTSVDPVREDASADGETRKALLRLADGHHIETVLMPHHGARNAVCVSTQAGCPMACAFCATGEMGLVRNLTAGEIVDQVRHWQRELAQRGERVSHVVFMGMGEPFNNYDATLASTRALIDPEAFGISPRRLTISTCGVVTGIDQLAGEGLPINLAVSLHAPDDPTRARIMPINRKWGVEEVLAAAARYVKRTKRRVTFEYVLLAGVNDSEETARELAALITRHGPTKDAHVNLIPYNPGAGGFRRPSVERMERFAALLQEHGIAATLRISKGQDIAAGCGQLKVA"
  },
  {
    "reference": "MKLIINDKTYTDNSGEADKNSIFVVSKHNEKYIQNAKENGCSEFLNSSELKNHLDMSSIKIIGITGTNGKTTTATTIYKILLSLGFKVALQGTRGFFINETQVEDYSLTTPIQLGNFVHIQKAIQNDCQFFVMEVSSHAIEQNRIEGLTFALKIHTNITRDHLDYHKTIEEYIRVKNSFLSDDSVKLVNIDDNVMKYNFTNAFTYSLKNLSDYKVSKYEFEHGMKVEFFYKEKTYTFTSMMMGIFNIYNLMASIAAVHITTQKSLEDICQTLKNFSCVSGRMEIVSLNPLVIVDFAHTPDGMDEVLKSFPNKDIICVFGAGGNRDSAKRPLMGEVAKKYSKHIIVTSDNPRFEEPKMIIEDILSGITNKSNVEVLIDRKEALKKAISLANEKSVILVLGKGDEATQIIGDKKFEFSDKDEVLKILNSKI",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. The designed protein should be involved in cell cycle, cell wall organization, regulation of cell shape, peptidoglycan biosynthetic process, cell division by localizing to cytoplasm and exhibiting magnesium ion binding, ATP binding, tetrahydrofolylpolyglutamate synthase activity.2. For general function, the protein need meet that Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.3. The presence of Mg(2+) is crucial for the protein to function optimally under specific conditions.4. The designed protein should have a binding site that is specific to the target UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate, meso-2,6-diaminoheptanedioate ligand.5. The designed protein have Mur ligase central, Mur ligase C-terminal domains with specific sequence motifs that are crucial for its activity.6. A protein that is capable of catalyzing reactions within the peptidoglycan biosynthesis, Cell wall biogenesis pathway with high efficiency and specificity.7. The protein design should include a Meso-diaminopimelate recognition motif motif to ensure proper function. The designed protein sequence is ",
    "response#1": "MKLRDLLRSVAIETVVGDTNVAVADDSRQIEAGDLFVAYPGDLADGRRYIADAVARGAVGVVWQPGGEFEWNPAWRVAQLAADDLRSLCGPLAALVADDSRQIEAGDLFVAYPGDLADGRRYIADAVARGAVGVVWQPGGEFEWNPAWRVAQLAADDLRSLCGPLAGRFYDNPSRRLKLVGVVTGTNGKTSVASFTRQIWEHLGLPAVNIGTTGVEGAFSAPLAHTTPEPVTLHRILAEIEAEDIQHAAMEASSHGLAQRRLDGVRLTAAAFTNISQDHLDYHADFEEYFAAKAGLFERVLPEDGVAVINTDGAGGDRMAAIAAARGQDVITVGRGAEADLRIEAQRFDATGQDLRFSFGGQRRQLRLELIGGFQAENALVAAGLAIACGADADTALAALADLEGVPGRMQLAATRENGAGVYVDYAHTPDAIATALRALRPHVMGRLVAVIGAGGDRDRAKRPLMGQAARAHADEVFVTDDNPRSEDPAAIRAEVLAGAPEAREVGDRAEAILRAVAGLGPGDAALIAGKGHETGQIIGDRVLPFDDREEVRAALARRQR",
    "response#2": "MKLNQILKNISVKEIIGPLDIEISSIEMDSRQVRAGSLFVCINGFTVDGHDFVEQAISNGASAILAERELNNRDIKIPYIVVGDTKRALAQLADYFYENPTKKLSIVGITGTNGKTTSTYMIKSILEHAGYNVGLIGTIANYIGDEKIKAERTTPESLELHELLYKMKERDVDYCVMEVSSHSLSLNRVYGLNFSQGIFTNLTQDHLDFHKNFENYYEAKLKLFKSAKNSIINIDDLYGKKMLQDIPQSSEKMTYSTEEGDIVAKNIVNHSRGAEFDLHCKGESIHIELSIPGRYNVYNALCSAGVSAVQYDSRKVNKGDLFVCIEGFNVDGHDYVESAIKNGAAVIICSKDVALNLKCAIVKVKDTREALAIIASNFYNIGLEEIKKGLENIKGILGRMELLGADQSVQVVVDYAHTPDALRALLAAARAHCAGKLWCVFGCGGDRDKGKRADMARIAEAMADQCVVTSDNPRTENPELIIQDVMAGFNEPDRVIQQVDRKAAIATAVAQAQAGDWVLVAGKGHEKYQIFKDKKIHFDDREEAKKILEENN",
    "response#3": "MKLRELIEPIVGIEVVGDANSLVVTGVTLDSREVNSGYVFLAMQGTREHGIRYLDQVLQAGAVAVLVEQADDLPAALPENAVVIDALSAKASEIYSNPSTKMKVVGVTGTNGKTTTTYLVRSIISNSDKKCGMIGTVTIDLGSGQDTIESKMTTPDALELASIAEKMATYGSEYMIMEASSHAIEQNRLAGIDFSAAAFTNFTGDHLDYHKTMEAYLAAKLRLFESLSPDATAVINSDDSVCEQVAAATKANKIFYSICGKGDLNAKLISMDINGTVYELSYRNEKVKVNSPLIGEHNLSNHLSAAGLAICLGTDPEVLFDVLPKLTGPVGRMERVDRGQDFVAVVDYAHKPAAIAAVLETLRTQLEGSTGRIGIVVGAGGDRDSSKRPLMGAAAAKGADLVVVTDDNPRTEDPASIRAAVLAGAQEANPAADIREVGSRAKAIDEVVAWAQPGDAVIVVGKGHETYQIVGDDRIEFDDAVVASQAVKDRRSP"
  },
  {
    "reference": "MRVKETQMNWPNLWKWGTLILGLVIICNASDKLWVTVYYGVPVWKDADTTLFCASDAKAHKTEVHNIWATHACVPTDPNPQEIPLENVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCSNMINKTDDAKNCTFHVTTEIRDKKQKVSALFYKLDIVQIEGSSNNSGNTSEYRLINCNTSVIKQACPKISFDPIPIHYCTPAGYAILKCNDKNFNGIGPCKNVSSVQCTHGIKPVVSTHLLLNGSLAEEEIIISSENLTNNAKNIIVHLNKSVEINCTRPSNNTRTSIHIGPGQVFYGRGDIIGDIRQAHCNVSGTEWYKVLRQVTGKLKEHFPNKIIVFEPPSGGDPEITLHHFNCRGEFFYCNTTKLLNKTCLNETMKNETECSNITLPCRIKQIINMWQGAGQAMYAPPIKGVINCVSNITGILLTRDGGNTSSETFRFGGGNIKDNWRSELYKYKVVQIEPLGVAPTRAKRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAVERYLKDQQFLGLWGCSGKIICTTSVPWNSSWSNKSFEEIWNNMTWTEWEREISNYTSQIYEILTKSQDQQDRNEKDLLELDKWASLWNWFDITNWLWYIKIFIIIVGGLIGLRIIFAVLSIVNRVRQGYSPLSLQTPFLQQREPDRPERIEEEGGEQDRDRSVRLVTGFLALAWDDLRSLCLFSYRRLRDFILIATRTVELL",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Transmembrane protein gp41: Acts as a class I viral fusion protein; Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.2. The designed protein should be involved in fusion of virus membrane with host endosome membrane, positive regulation of receptor clustering, virion attachment to host cell, positive regulation of plasma membrane raft polarization by localizing to viral envelope and exhibiting structural molecule activity.3. The designed protein must have at least one Retroviral envelope protein GP41-like domain.4. The protein should have a Helical domain that is long enough to span the entire membrane.5. Ensure the presence of a signal peptide within the protein. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWKDADTTLFCASDAKAHETEVHNIWATHACVPTDPNPQEIPLENVTENFNMWKNKMAEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTKVTLKSTTNVTVNSTTNVSNVSDFANEVRNCSFNMTTEIRDRKQRVHALFYRLDLVPMNNDNNNSEYRLINCNTSVIKQACPKITFDPIPIHYCTPAGYAILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEKEIIIRSENLTNNAKTIIAHFNKSVVINCTRPFNNTRTSVTIGPGQVFYRTGDITGDIRKAYCEINGTNWFEALKQVAGKLKEHFPDKSIAFQPHSGGDLEITTHHFICRGEFFYCNTSQLFNITYTEGNETIEESNGNITLPCRIKQIINMWQGVGQAMYAPPVSGKINCVSNITGILLTRDGGAGTGDNNTETFRPAGGNMKDNWRSELYKYKVVQIEPLGLAPTRAKRRVVEREKRXXXXXGVXALFLGFLSAAGSTMGAASVTLTVQARQLLSGIVQQQNNLLKAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLDNIWNNMTWMEWEREIDNYTDLIYTLLVASQNQQEKNEQELLELDKWASLWNWFDITQWLWYIKIFIMIVGGLVGLRIVFTVLSLVNRIRQGYSPLSFQTRLPAQRGPDRPDGTEEEGGERDRDRSGQLVDGLLALIWVDLRSL",
    "response#2": "KEEVRNCSFNVTPSIKNKMQKEYALLYKLDLVAINKTDNTSYRLISCNTSTLTQACPKVSFEPIPIHYCAPAGFAIIKCNDKKFSGKGSCTNVSTVQCTHGIRPVVSTQLLLNGSLAEDEIVIRSENITDNAKTIIVHLSEAVQINCTRPNNNTRKSITIGPGRAFYATGEVTGDIRQAHCNISGTEWNNTLRQVSEKLRDKFKNKTIVFKPSSGGDLEIEMHSFNCGGEFFYCNTTKLFNSSWPNSTRNNTEESNSTVITLPCRIKQIINMWQEVGKAMYAPPIRGRINCTSNITGLLLTRDGGNGNSTNETFRPQGGNIRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR",
    "response#3": "LWVTVYYGVPVWKEATTTLFCASDAKAYATEVHNVWATHACVPTDPNPQEVVLGNVTEDFNMWENNMVDQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDPNCTDAGNYLNCNNTGSNISYSSNGKKMEVDIKNCSFNITTSMRDKMQKGYSVFNKLDIVPIDNDRTNTSYRLINCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNNKTFEGKGQCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVIIRSDNFSNNAKTIIVQLNKPVVINCIRPNNNTRKSIHIGPGRAFYTTDIIGNIRRAHCEINKTEWDEALNQTAIKLKEQYNTTTIIFRQSAGGDPEIVMHTFNCGGEFFYCNTTQLFDSTWNENSTQLSNNTGNDTITLPCKIKQIINMWQEVGKAMYAPPISGPIRCSSNITGLLLTRDGGNEDNGGPETFRPGGGNMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAALGALXLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHMLKLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTNVPWNSSWSNKSQDEIWNNMTWLQWDKEISNYTNLIYSLIEESQNQQEKNEQDLLALGKWANLWTWFDISNWLWYIRIFIMIVGGLIGLRIVFAVLAVIKRVRQGYSPVSFQTRLPAQRGPDRPEGIEEEGGERDRDRSRPLVDGFLAIIWIDLRSL"
  },
  {
    "reference": "MKKSQYFIVFICFFVLFSVHPIAAAAADSDNSTVNEWFQKKDEKTADQSEQKKEKTTKTADETEGAAAPSVSAFDFVKMIFALLFVIVLIYGLVKLMNKRNRLLKPFQYVENIGGTSVGQNRSIQLIKVGKSVLVVGVGETIQLLKEIEDEKEIEVILSQHEEAMSSKIEWQKFVKPLKSSEHQPQQKLPSFSKALKEQLEELKQNRSEGKKKGPRHHE",
    "instruction": "Create a protein sequence that satisfies the following specifications: 1. Ensure the presence of a signal peptide within the protein.2. The protein should have a specific function in bacterial-type flagellum organization and should be localized to plasma membrane in order to carry out this function efficiently. The designed protein sequence is ",
    "response#1": "TRNIVLVGPTGVGKTTTLAKLAAFYFVTEPKRTGIQPRVKIITTDNFRIGAAFQMERYCELMGLDLCVVQAPVEFLTYMTLYQQETDVVFVDTEGRSPVDGQNIERMVEYFRAVKNFELEVYLTIDAGSKANDLREVFKQYALFEYRALIVTKLDETTSIGNLISALSEARTPITYITTGQTVPSNLEKASVNLLLSKLKQ",
    "response#2": "TRNIVLVGPTGVGKTTTLAKLAAFYFVTEPKRTGIQPRVKIITTDNFRIGAAFQMERYCELMGLDLCVVQAPVEFLTYMTLYQQETDVVFVDTEGRSPVDGQNIERMVEYFRAVKNFELEVYLTIDAGSKANDLREVFKQYALFEYRALIVTKLDETTSIGNLISALSEARTPITYITTGQTVPSNLEKASVNLLLSKLKH",
    "response#3": "NIVLVGPTGVGKTTTLAKLAAFYFVTEPKRTGIQPRVKIITTDNFRIGAAFQMERYCELMGLDLCVVQAPVEFLTYMTLYQQETDVVFVDTEGRSPVDGQNIERMVEYFRAVKNFELEVYLTIDAGSKANDLREVFKQYALFEYRALIVTKLDETTSIGNLISALSEARTPITYITTGQTVPSNLEKASVNLLLSKP"
  },
  {
    "reference": "MPLQAIVSFLRLDIAAGVILVGAAVLALIAANSPAAALYEQVFQTPFVIGYGPWLLEKPLLLWINDGLMAVFFLLVGLEIKREVRGGELSTPRLAALPAVAAVGGMVVPALIYASLTWGDAFALRGWAIPAATDIAFALGILTLLGPRVPISLKIFLTALAIIDDLGAILIIAFFYTASLSPLALLLAAACLALLIGLNLSGQRRLWPYLLIGVVLWVCVLKSGVHATLAGVVLALTIPLGATDDESASADKPLERLEHGLHPWVTYAILPLFAFANAGVSLAGLPPSALLAPVPLGIVLGLFLGKQIGVFGFSWLAIRSGLAPMPQGARWRDLYGVALITGVGFTMSLFIGTLAFETSDPLAADFGTEVRLGVLSGSLLSGVIGYLVLRLSRRNPATE",
    "instruction": "Synthesize a protein sequence with the appropriate folding and stability properties for the desired function. 1. For general function, the protein need meet that Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons.2. The designed protein localizes to the plasma membrane.3. The protein should have a Helical domain that is long enough to span the entire membrane. The designed protein sequence is ",
    "response#1": "MTCVRP",
    "response#2": "GQTXPXGX",
    "response#3": "MMSLPR"
  },
  {
    "reference": "MKYTKQEARKRRHYRVRSKVSGTAAKPRLNVFKSNTNFYAQIIDDTTGTTLVSASSLNLGLKSGNIEAAKKVAEEIAKLAIAKSIVDVVFDRGGYLYHGKVKAFAEAARENGLKF",
    "instruction": "Create a protein sequence with the necessary features to perform the desired function. 1. The designed protein's functionality will benefit from Basic residues compositional bias in its structure.2. A protein that localizes to cytoplasm, ribosome and can perform rRNA binding with high specificity and efficiency. The designed protein sequence is ",
    "response#1": "PGYPGKNHKQYATPKRRFEKTRLEDEKRLIIDYGLRNKRELWKAQSVLRKYRAGARELVALRSGGLSTEDYLKKRDQLVNHLYRYGLVGENADVGDVLALKVEQQLA",
    "response#2": "MATNAA",
    "response#3": "RRLQTLVLRRGFARSPKQARQFITHGHIAIKGRRVTIPGYRVERAEEAEISYYGPSPLTNEVL"
  },
  {
    "reference": "MNFSKNIVFWIVILFLLFALFNVFQGPQQQGIQSKLAYSEFITAVENGEVQDVTIKGTKAGSNVFGHLSDGRTFTTFAPEDPALVRTLTENQVRIQAAPSDEGQPTLMSFLISLFPFLLLIGVWIFFMRQMQGGGAGGKAMGFGKSKAKLLTEKQGRITFEDVAGIDEAKQELEEIVEFLKNPQKFQRLGGKIPKGCLLVGPPGTGKTLLARAIAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLNQLLVEMDGFESNEGVILIAATNRPDVLDPALLRPGRFDRQVQVPNPDILGREKILKVHMKKVPLAPDVIARTIARGTPGFSGADLANLVNEAALLAARTGKRVVTMSDFESAKDKVMMGAERRSMVMTEEEKKLTAYHEAGHALVGLHMPKSDPLHKVTIIPRGRALGVTMSLPERDQYTFSKIQLESKLAMMFGGRVAEEIIFGKENITTGAGNDIQQATAMARRMITEFGFSDKLGPLRYNANEEEVFLGHSVTQQTNISEETANLIDQETRRLIDEAEGHARRVLTDHKNQLEDITQALLEYETLNGQEVRDIMDGKGVNRPDPDDLSDQGGPKTSVPSSGKGSSKDKDDAGIDADPLPEA",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The ideal protein would be one that plays a critical role in proteolysis through metalloendopeptidase activity, zinc ion binding within plasma membrane.2. The active site active site of the enzyme must be accessible to solvent molecules for proper catalysis to occur.3. The protein must exhibit the following characteristics: Plays a role in the quality control of integral membrane proteins.. The designed protein sequence is ",
    "response#1": "VILVGPPGTGKTMLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEDAKKNAPCIVFIDEIDAVARRRGTGMGGGHDEREQTLNQLLVEMDGFGVNEGIIVMAATNRVDILDPAILRPGRFDRKVAVTF",
    "response#2": "VLLVGPPGCGKTLLARAIAGEADCPFFSISGSDFVEMFVGVGASRVRDLFKQAKENSPCIIFLDEVDAVGRRRGSGFSSGGHDEREQTLNAILVEMDGFDATDQVIVIAATNRSDVLDPALTRPGRFDRQVPVLFLGY",
    "response#3": "VLLVGPPGCGKTLLARAIAGEADCPFFSISGSDFVEMFVGVGASRVRDLFKQAKENSPCIIFLDEVDAVGRRRGSGFSSGGHDEREQTLNAILVEMDGFDATDQVIVIAATNRSDVLDPALTRPGRFDRQVPVLHFDVDLDIIAAAAVGLTGADIRNIVNEAALWAARNDKSVVSMADFL"
  },
  {
    "reference": "MESRAEGGSPAVFDWFFEAACPASLQEDPPILRQFPPDFRDQEAMQMVPKFCFPFDVEREPPSPAVQHFTFALTDLAGNRRFGFCRLRAGTQSCLCILSHLPWFEVFYKLLNTVGDLLAQDQVTEAEELLQNLFQQSLSGPQASVGLELGSGVTVSSGQGIPPPTRGNSKPLSCFVAPDSGRLPSIPENRNLTELVVAVTDENIVGLFAALLAERRVLLTASKLSTLTSCVHASCALLYPMRWEHVLIPTLPPHLLDYCCAPMPYLIGVHASLAERVREKALEDVVVLNVDANTLETTFNDVQALPPDVVSLLRLRLRKVALAPGEGVSRLFLKAQALLFGGYRDALVCSPGQPVTFSEEVFLAQKPGAPLQAFHRRAVHLQLFKQFIEARLEKLNKGEGFSDQFEQEITGCGASSGALRSYQLWADNLKKGGGALLHSVKAKTQPAVKNMYRSAKSGLKGVQSLLMYKDGDSVLQRGGSLRAPALPSRSDRLQQRLPITQHFGKNRPLRPSRRRQLEEGTSEPPGAGTPPLSPEDEGCPWAEEALDSSFLGSGEELDLLSEILDSLSMGAKSAGSLRPSQSLDCCHRGDLDSCFSLPNIPRWQPDDKKLPEPEPQPLSLPSLQNASSLDATSSSKDSRSQLIPSESDQEVTSPSQSSTASADPSIWGDPKPSPLTEPLILHLTPSHKAAEDSTAQENPTPWLSTAPTEPSPPESPQILAPTKPNFDIAWTSQPLDPSSDPSSLEDPRARPPKALLAERAHLQPREEP",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must have at least one cDENN, uDENN, dDENN domain.2. The protein must exhibit the following characteristics: Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.; Guanine nucleotide exchange factor (GEF) which may activate RAB8A, RAB13 and RAB35.3. Incorporating a Clathrin box, FXDXF motif motif is critical for the successful design of the protein.4. The designed protein should be involved in endocytosis, endocytic recycling by localizing to nucleoplasm, centrosome, cytosol, clathrin-coated vesicle, intracellular membrane-bounded organelle and exhibiting guanyl-nucleotide exchange factor activity, phosphatidylinositol phosphate binding.5. The designed protein's functionality will benefit from Polar residues, Basic and acidic residues, Pro residues compositional bias in its structure. The designed protein sequence is ",
    "response#1": "LPEVYCIVSRLGCFSLFSKILDEVEKRRGISPALVQPLMRSVMEAPFPALGKTIIVKNFLPGSGTEVIELCRPLDSRLEHVDFESLFSSLSVRHLVSVFASLLLERRVIFIADGYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKAEGGAGPRGKEGAHAPCASEEAATESSESGSTLQPPSADSTPDVNQSPRGKRRAKAGNRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKPGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQEVQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQETPLLLGRFSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQLPEVYCIVSRLGCFSLFSKILDEVEKRRGISPALVQPLMRSVMEAPFPALGKTIIVKNFLPGSGTEVIELCRPLDSRLEHVDFESLFSSLSVRHLVSVFASLLLERRVIFIAD",
    "response#2": "MEELIPLVNKLQDAFSSIGQSCHLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLILQLIFSKTEHAEFLHCKSKKFTDFDEVRQEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLIDLPGITKVPVGDQPPDIEYQIKDMILQFISRESSLILAVTPANMDLANSDALKLAKEVDPQGLRTIGVITKLDLMDEGTDARDVLENKLLPLRRGYIGVVNRSQKDIEGMEELIPLVNKLQDAFSSIGQSCHLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLILQLIFSKTEHAEFLHCKSKKFTDFDEVRQEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLIDLPGITKVPVGDQPPDIEYQIKDMILQFISRESSLILAVTPANMDLANSDALKLAKEVDPQGLRTIGVITKLDLMDEGTDARDVLENKLLPLRRGYIGVVNRSQKDIEGMEELIPLVNKLQDAFSSIGQSCHLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLILQLIFSKTEHAEFLHCKSKKFTDFDEVRQEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLIDLPGITKVPVGDQPPDIEYQIKDMILQFISRESSLILAVTPANMDLANSDALKLAKEVDPQGLRTIGVITKLDLMDEGTDARDVLENKLLPLRRGYIGVVNRSQKDIEGMEELIPLVNKLQDAFSSIGQSCHLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLILQLIFSKTEHAEFLHCKSKKFTDFDEVRQEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLIDLPGITKVPVGDQPPDIEYQIKDMILQFISRESSLILAVTPANMDLANSDALKLAKEVDPQGLRTIGVITKLDLMDEGTDARDVLENKLLPLRRGYIGVVNRSQKDIEG",
    "response#3": "LPEVYCIVSRLGCFSLFSKILDEVEKRRGISPALVQPLMRSVMEAPFPALGKTIIVKNFLPGSGTEVIELCRPLDSRLEHVDFESLFSSLSVRHLVSVFASLLLERRVIFIADDARDVLENKLLPLRRGYIGVVNRSQKDIEGKKDIRAALAAERKFFLSHPAYRHMADRMGTPHLQKTLNQQLTNHIRESLPALRSKLQSQLLSLEKEVEEYKNFRPDDPTRKTKALLQMVQQFGVDFEKRIEGSGDQVDTLELSGGARINRIFHERFPFELVKMEFDEKDLRREISYAIKNIHGVRTGLFTPDLAFEAIVKKQVVKLKEPCLKCVDLVIQELINTVRQCTSKLSSYPRLREETERIVTTYIREREGRTKDQILLLIDIEQSYINTNHEDFIGFANAQYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKAEGGAGPRGKEGAHAPCASEEAATESSESGSTLQPPSADSTPDVNQSPRGKRRAKAGNRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKPGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQEVQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQETPLLLGRFSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQ"
  },
  {
    "reference": "MTTIVSVRRGNQVVIAGDGQVSLGNTVMKGNAKKVRRLYNNKVLAGFAGGTADAFTLFERFESKLEIHQGNLTRAAVELAKDWRTDRMLRKLEALLAVADETASFIITGNGDVVQPENDLIAIGSGGPFAQAAASALLDNTELSAEEIAEKALTIAGNICVFTNHNHTVEKIDY",
    "instruction": "Synthesize a protein sequence that satisfies the given functional constraints. 1. The protein should be optimized for proteasome core complex-specific folding and stability, while maintaining metal ion binding. The designed protein sequence is ",
    "response#1": "VGLEANLPNAYCNAMLQALYFIVPLRKAILSHCCSKEFCLACELGFLFHMLDVGTSSQPCQASNFLRSFRTVPEAAALGLILSSDRSNNASINLISLIQNWNRFILHQIHYELLEAIKKNENMMTVSEPVGVGRRMQLSASPRPFIPGAEMHVQHPEDYQETLDNEVQQYESNNEKFQFLEASSDYDVTLIPECEPYPDPAEQMPPSSKNYNKDETEISRLFGTQQKTTQRCLKCNAERVKNNILLVCNMLYPVNANNC",
    "response#2": "VGLEANLPNAYCNAMLQALYFIVPLRKAILSHCCSKEFCLACELGFLFHMLDVGTSSQPCQASNFLRSFRTVPEAAALGLILSSDRSNNASINLISLIQNWNRFILHQIHYELLEAIKKNENMMTVSEPVGVGRRMQLSASPRPFIPGAEMHVQHPEDYQETLDNEVQQYESNNEKFQFLEASSDYDVTLIPECEPYPDPAEQMPPSSKNYNKDETEISRLFGTQQKTTQRCLKCNAERVKNNILLVCNMLYPVNANNC",
    "response#3": "VGLEANLPNAYCNAMLQALYFIVPLRKAILSHCCSKEFCLACELGFLFHMLDVGTSSQPCQASNFLRSFRTVPEAAALGLILSSDRSNNASINLISLIQNWNRFILHQIHYELLEAIKKNENMMTVSEPVGVGRRMQLSASPRPFIPGAEMHVQHPEDYQETLDNEVQQYESNNEKFQFLEASSDYDVTLIPECEPYPDPAEQMPPSSKNYNKDETEISRLFGTQQKTTQRCLKCNAERVKNNILLVCNMLYPVNR"
  },
  {
    "reference": "MPASAHQLDLADPLAHYREQFYLPGGRIYFDGNSLGLLCRAAERAVLDTLQQWKELAIEGWTAAEPDWFHLTEQLAAQLCRIVNAPDHSITLTGSTTLHLQQVLATLYRRDDPRRGILIDSTAFPTDRYAVAGFLRSQGLDPSVHLLEVPVDAAGLLQEDAIAAALTQGVQMAVLPSVVFTTGQLLDLQSITSVAAGHDVRICWDCSHSVGVVPLNLSELQPDAAIWCTYKYLNGGPGSVAAMYLHPRWHDLPPGLAGWFGADKSRQFRMEHELVPAVGAGRLQLGTPHVLSLAALRGSLQLIEAAGVERLRQKSLQLTARLRQLLHEQLPDAGFVCATPEADHRRGGHLAIRHPLARQLSLKLRSRGFVPDFREPDLLRIAPSPLYSRFSDCDDLVVEIGRSLAAGFEAVDLAQSLVT",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. We require a protein that can specifically bind to the metabolites produced in the quinolinate from L-kynurenine: step 2/3 pathway.2. A protein that contributes to 'de novo' NAD biosynthetic process from tryptophan, tryptophan catabolic process, L-kynurenine catabolic process, anthranilate metabolic process by functioning in cytoplasm with 3-hydroxykynureninase activity, kynureninase activity, pyridoxal phosphate binding.3. The protein must exhibit the following characteristics: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively..4. The presence of Aminotransferase class V domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "ARARHGLANIVGAHPEEVTVANALTVNLHLLMVSFFRPTGKRRKIIMEAGAFPSDQHAIISQLKFHGLNPDDCLIEVAPRAGEWTIRTEDIVTQIEGVGEELALVLWSGIHYYTGQFFDIQAIADAGAAAGARVGIDLAHAAGNVPLHLHDWGVDFAVWCNYKYLNGGPGAPGGLFVHERHAEDDSLPRFAGWWGHRQSDRFQMRREFRPERGAAGWEVSTVPVLGMAPLLASLPLFAEAGGMSALRKRSELLTQQLYDGIAAINGLTILTPADPQARGAQLSVYVPGHRPDLRELDDLVWIAPEQTSKLVGSLPSEVVVMETLTANLHLLMASFYRPTADRYKIIIEGKAFPSDHYAAQSQLAHHNIPPSALITIEPPSSSSPYLSTEHILSIISQHAASTALVLLPGIQYYSGQLFDIELITRHCHSLGIMVGWDLAHAVGNVPLKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHV",
    "response#2": "MKLGRGWVKIQDHTTPLLADLVGAREKEVACMGTLTTNLHLMMNTFYKPSSDRYKILCEAKAFPSDQYAFASQAHLHGYDPKEAIIELSPREGEYSLRPNDILDVIAREGSSIALVLFSGIQYYTGQLFPMESITRAAKEQGCICGWDLAHAIGNVPLSLHDWDADFAVWCTYKYLNSGPGGIGGLYIHEKWDDATKQQSAKIVGALPSEVVVMETLTANLHLLMSSFYRPTADRWKIIIEGKAFPSDHYAALSQIAHHNLDPSALITIEPPSASSPYLSTEHILSVISKHAATTALVLLPGIQFYSGQFLDIELITRHCHSNGIPIGWDLAHAVGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHEATKQQSAKIVGALPSEVVVMETLTANLHLLMSSFYRPTADRWKIIIEGKAFPSDHYAALSQIAHHNLDPSALITIEPPSASSPYLSTEHILSVISKHAATTALVLLPGIQFYSGQFLDIELITRHCHSNGIPIGWDLAHAVGNVPVKLHDWNVDFAAWCNYKYMNGGPGVIGGLFVHEG",
    "response#3": "ARARHGLANIVGAHPEEVTVANALTVNLHLLMVSFFRPTGKRRKIIMEAGAFPSDQHAIISQLKFHGLNPDDCLIEVAPRAGEWTIRTEDIVTQIEGVGEELALVLWSGIHYYTGQFFDIQAIADAGAAAGARVGIDLAHAAGNVPLHLHDWGVDFAVWCNYKYLNGGPGAPGGLFVHERHAEDDSLPRFAGWWGHRQSDRFQMRREFRPERGAAGWEVSTVPVLGMAPLLASLPLFAEAGGMSALRKRSELLTQQLYDGIAAINGLTILTPADPQARGAQLSVYVPGHRPDLLARLAP"
  },
  {
    "reference": "MQDGRTTLSKFLIDTLDRQPCSTETARNAGLSALLIDVAAAIKSISAMLTKGALGGNYGSAQSINTHGEEQKKLDVATNEIFVQQCEWDGLLAAMVSEEMESVYAIPPGYPRGDYLLAFDPLDGSSNIDINGVVGSIFSVLRNGEGNGNANEKNGSDPRGTVGESAFLRPGCEQVAAGYAVYGPSTMLVLSVGNGTHGFTLEREIGNFVLTHSNIRIPEDTVEFAINASNERFWEPPVRRYVQECKDGRSGCRASDFNMRWIASMVAEVHRILMRGGVFMYPRDSKTPAMEGRLRLLYEANPMSFLVEQAGGLSITGRERILDVVPRALHGRVPVILGSKHEVERIGRYHGEYDRGEDQPFTSPLFSRRSLFLPGFTA",
    "instruction": "Construct a protein sequence with the desired structural and functional characteristics. 1. The protein design must be focused on enhancing the efficiency of gluconeogenesis.2. The protein must be engineered to have a high affinity for Mg(2+) in order to increase its catalytic activity. The designed protein sequence is ",
    "response#1": "DTHKYRRGHAAVFSGGATSTGAARLSAMAAARSGAGAVTVLSPGEALAANAAHLTSIMLSRCDHGQDLADFIEARRPTAFVIGPGFGIGEKTRALALDLLDKASGHFVFDADTITSFKDKPDMLFAATRRLPEVRLVLTPHEGEFKRLFPDLDAEAMPSKLERARAAAARANAIIVYKGADTVIAAPDGRAVINSNGTPLLATAGSGDVLAGLNAGLMAQGR",
    "response#2": "IPADTKEFAINASRERFWEPPVKRYIDECRQGKEGPRGKDFNMRWVASMVAEVHRILCRGGVFLYPADTENMKKGGKLRLMYEANPMAFIVEQAGGAATTGRGRMMEVPPTGLHQRVPVILGSKEEVERIGAYL",
    "response#3": "VPENTQEFAINASNSRFWEPPVKRYVDECLAGKTGPRDKDFNMRWIASMVAEAHRILMRGGVFLYPRDSKDAAKPGRLRLLYEANPIGFIMEQAGGRASTGREPMLGVQPTSLHQRIGLIFGSKNEVERIERYL"
  },
  {
    "reference": "MEWKKGRRILLGITGGIAAYKVPDLVRRLRSAGNEVEIILTESAERLVSPLVLSTLSGRRVWLQKDFLDFENGWKIPHITLAEWAEIVCIAPCTANMMRRAANGEAETLLGAALLATRAPVLLCPAMNVNMWEHETTMRHKEACLRNGYRVLEPDTGFLACGTEGRGRLPSIEVILEEIWRILCPVRDLEGKKVLVTAGPTWEFLDPVRFISNPSSGRMGYAIAQNAWYRGAEVTLVSGPVSIQPPHGVALVRVQSALDMLEAVLEQAERADFIVKAAAVGDYRPKEFSAEKIKRGGKNSLVVQFEQNPDIAATIGARKKPGQVLIGFAAESHALEKNAAEKLVSKNLDFIVANRITGPDGAFGSEHNTIHILDQKGGKETFSGSKEEVAWRLWDFILEAGSAGAPRN",
    "instruction": "Design a functional protein sequence with the following characteristics: 1. The designed protein should interact with enzymes and substrates within the CoA from (R)- pantothenate: step 3/5, Cofactor biosynthesis pathway to enhance or regulate its activity.2. The Proton donor active site of the enzyme must contain residues that facilitate the binding and orientation of the substrate.3. The protein must exhibit the following characteristics: In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4- phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine..4. The stability of the protein-Mg(2+) complex should be optimized to maximize enzymatic activity.5. The presence of Flavoprotein, DNA/pantothenate metabolism flavoprotein C-terminal domains is critical for the interaction of the designed protein with its intended targets.6. The CTP binding site should be located in a region of the protein that is accessible to the ligand.7. The designed protein should have phosphopantothenoylcysteine decarboxylase activity, FMN binding, phosphopantothenate--cysteine ligase activity, metal ion binding to facilitate pantothenate catabolic process, coenzyme A biosynthetic process. The designed protein sequence is ",
    "response#1": "KNILLGISGGIAAYKSAELVRLLIKQGANVRVCMTKSAQQFITPLTMQALSGNPVHTELLDPEAEMGMGHIELARWADYILIAPATANTLAKLAWGLADELLTTICLASXAPLAVAPAANQQMWKNPATQHNIHILKERGVYVFGXAEGEQACGDTGQXRMLEPAELLAAAAAHFGSLRGRQIVISAGGTREAIDPVRFIGNRSSGRFGVEIARAAQAAGAHVTLVAANIDGQILNTLEHTDIELVRVESAQELQTAMLETAASADVIVMSAAVADYRPANRTATKQKKDGTGRKTIELVENPDILAELTHTRRREGQIIIGFAAETGDAEASVLEYGRAKAARKGADLMVINEVGTERGFGEVETAITILFANGASPTLTASAKPEIAHALLDAINAAYS",
    "response#2": "KRILLGITGGIAAYKAAELVRELRKAKAEVRVVMTTAAQEFITPLTLQALSGNPVHYSMLDPEAEAGMGHIELARWADIIVVAPATADFIARLTHGMGNDLLTTLCLASEAPLAICPAMNQAMWRDEMTQANIQRLIDIKGKKFHSFGPADGEQACGDVGPGRMLEPTLIAEQCEAVLRHPRDLRGRQIVISAGGTREAIDPVRFIGNRSSGRFGVEIARAAQAAGAHVTLVAANIDGQILNTLEHTDIELVRVESAQELQTAMLETAASADVIVMSAAVADYRPANRTATKQKKDGTGRKTIELVENPDILAELTHTRRREGQIIIGFAAETGDAEASVLEYGRAKAARKGADLMVINEVGTERGFGEVETAITIVADSGPETAIPHGSKDDLADQIWDTVRERLP",
    "response#3": "KRILLGITGGIAAYKAAELVRELRKAKAEVRVVMTTAAQEFITPLTLQALSGNPVHYSMLDPEAEAGMGHIELARWADIIVVAPATADFIARLTHGMGNDLLTTLCLASEAPLAICPAMNQAMWRDEMTQANIQRLIDIKGKKFHSFGPADGEQACGDVGPGRMLEPTLIAEQCLALFTAASLAGARVLVTAGPTREAIDPVRFLSNRSSGKMGYALAAAASRRGAKVTLVSGPTALAAAPGVELVPATSAAEMARAVRSRFSRCDALLMAAAVADFTVQRPARSKIKKDRIRGPLRLALVPTEDILASLGRSRRGRRPWLIGFAAETGDPVPAAKDKLARKRLDMVVANDVSEKGAGFEVDSNHLYLFWHDGERALPLAPKAVLARELIAHIAGRLNAKP"
  },
  {
    "reference": "MRVKETQMNWPNLWKWGTLILGLVMICSASNNLWVTVYYGVPVWRDADTTLFCASDAKAHDTEVHNVWATHACVPTDPNPQEIHLENVTENFNMWKNNMVEQMHEDVISLWDQSLKPCVKLTPLCVTLECENATWTNRNNIITDNGKIGNLTDEVKNCTFNMTTELEDKKQKVQALFYKLDIVQMHNSNNDSNYYRLINCNTSVIKQACPKISFDPIPIHYCTPAGYVLLKCNDKKFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKTIIVHLNKSVKINCTRPSNNTRTSTTIGPGQTFYRTGDIIGDIRKAFCEINGTEWNEVLGKVKEKLNEHFSNKTIFFQPPLGGDLEIRTHHFNCRGEFFYCNTTKLFNNNCTGSIANTSMEGCNNTIILPCRIKQIINMWQGVGQAMYAPPISGSINCVSNITGILLTRDGGINNKTNGTEIFRPEGGNIKDNWRSELYKYKVVEIEPLGIAPTKAKRRVVEREKRAVGIGAMIFGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLRAIEAQQHLLQLTVWGIKQLQARVLAVERYLKDQKFLGLWGCSGKVICTTAVPWNSSWSNKTFDEIWNNMTWIEWEREISNYTNQIYNILTESQNQQERNEKDLLELDKWTSLWNWFNITNWLWYIKIFIIIVGSIIGLRIIFAVLSIVNRVRQGYSPLSFQTPTHHQREPDRPEEIEEGGGEQGRGRSVRLVSGFFALAWDDLRSLCLFS",
    "instruction": "Design a protein sequence that is tailored to the desired application. 1. For general function, the protein need meet that Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.2. The designed protein localizes to the plasma membrane. The designed protein sequence is ",
    "response#1": "LWVTVYYGVPVWRDADTTLFCASDAKAHVTEVHNVWATHACVPTDPNPQEIPLKNVTERFNMWENNMVEQMQEDVISLWDESLKPCVKLTPLCVTLHCTNANLSQVNTTDRNMIGDIGNITEEISNCTFNMTTEIRDKKHKVHALFYKLDIVKIEDKNNGSGYRLINCNTSVIKQACPKISFDPIPIHYCTPAGYALLKCNEKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIISSENLTNNAKNIIVHLNRSVEINCTRPSNNTRTSINIGPGQVFYKTGDIIGDIRKAYCEINGTKWKEVLEQVAAKLREHFPNKTIVFQPPSGGDLEITMLHFNCRGEFFYCNTTKLFNNTYNGTMEELNGTIILPCRIKQIVNMWQGAGQAIYAPPIKGSIRCVSNITGILLTRDGGNKASTNETETFRPGGGNIKDNWRSELYKYKVVQIEPLGVAPTKAKRRVVEREKR",
    "response#2": "GEMKNCSFNVTTEVRDKVKKEYALFYTLDIVQIDNTSYRMTSCNTSVITQACPKVTFEPIPIHYCAPAGFAILKCKDKKFKGTGSCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVVIRSENFTDNTKNIIVQLNEAVEINCTRLNNNTRKSIHIGPGRAFYTTGDIIGDIRQAHCNLSRAAWNTTLQKIAEKLTEQYGNKTISFNRSSGGDLEVVMHNFNCGGEFN",
    "response#3": "MGAASITLTVQARQLLSGIVQQQNNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLICTTTVSWNSTWSNKSYSEIWDNMTWMQWDREIINYTEQIYXLIEKAQHQQEXNEQELLALDKWAGLWNWFDISNWLWYIXIFIMIVGGLIGLRIVFTVLSIINRVRQGYSPLSLQTY"
  },
  {
    "reference": "MRAAILFCLVASSMAVPSGSLGSRPGTCPPQPSDQVMIEATRCSYVYGLTWDWNCNSQGQENYKCCQYENDIRICVPPIPADVDEEVGVEQPSQSVDQVRQAIQKTQDFIRKVGLYPAPDQRRRTTPTPDTVRWCVSSRCQMTKCQRMVSEFTYSPNMVPRKQWKCTQATSQEQCMFWIEQGWADIMTTREGQVYSANTTFNLKPIAYETTINDQQPEIQILKHYQNVTFALKSSRLVNPNTFSELRDKTTCHAGIDMPDFDMPASFADPVCNLIKEGVIPVTGNYIESFSDFVQESCVPGVLNMTYNKNGTYPLSLVTLCEDQQYKYSGIKGALSCLESGKGQVTFVDQKVIKKIMSDPNVRDNFQVVCRDESRPLDEEIFTDVTCHVGHTARPTIFINKNNTQQKETDIKTLVVKMMELYGNTDRDVNFNIFDSSVYDCGKCQMTGKPLNKNLIFLEESNTMKIVDDSKVYAGEVYAAYNVCSKLVPKPRAKICVTNVTEYEACRRFKGIAENIPEVKKVAWGCVLANSSIECMQAVHNNTADLFKANPMETFIAGKEFLLDPLMSVHRNDSVTMNHTYTRTLAVIKRSSLAKFPGLLSVPEGQPKYIKDLWKLKICSAGLKNFSAFHSPIGYLLANGTIPRIGSVFESVNRYFQATCVPEIEPETWRLDSDLLLGREMNWGFSSLNMYNFTGQEWLLWNTPATWNFLTYNRKVSTGLDIKKLIELKKQNLTSHIFNQNLSSPRNVELLDDLVGVEGISDLVKGVQ",
    "instruction": "Generate a protein sequence that meets the functional requirements while minimizing unwanted side effects. 1. The protein must exhibit the following characteristics: May serve the following two functions: a classical role as a yolk protein precursor and probably shuttle iron to developing germ cells..2. The designed protein should be involved in iron ion transport by localizing to extracellular space, recycling endosome, early endosome, plasma membrane and exhibiting metal ion binding, nutrient reservoir activity.3. The presence of Transferrin-like domains is critical for the interaction of the designed protein with its intended targets. The designed protein sequence is ",
    "response#1": "VRWCVKSEKELKKCHDLAANVAGFSCVRRDDSLECIQAIKREEADAITLDGGDIYIAGLHNYNLQPIIAEDYGEDSDTCYYAVAVAKKGTEFGFLDLRGKKSCHTGLGKSAGWNIPIGTLVTVGQIQWAGIEDRPVESAVSDFFNASCAPGANRDSQLCQLCMGDCSRSHNEPYYDYSGAFQCLKDGAGEVAFIKHLTVPAAEKASYELLCKDNTRAPIDSYKTCHLARVPAHAVVSRKDPRLANLIYSKLMAVTNFNLFSSDGYAAKNLMFKDSTQNLVQLPMTTDSFLYLGAEYMSTIRSLTKAQATGVTSRC",
    "response#2": "YKLCVPAAYMKDCEQMLEVPTKSKVALECVPARDRVECLSFVQQRQADFVPVDPEDMYVASKIPNQDFVVFQEYRTDEEPDAPFRYEAVIVVHKDLPINNLDQLKGLRSCHTGVNRNVGYKIPLTMLMKRAVFPKMNDHSISPKENELKALSTFFAKSCIVGKWSPDPKTNSAWKSQYSHLCSMCEHPERCDYPDNYSGYEGALRCLAHNNGEVAFTKVIFTRKFFGLPVGTTPASPSNENPEEFRYLCVDGSKAPITGKACSWAARPWQGLIGHNDVLAKLAPLREKVKQLADSGAADKPEWFTKVLGLSEKIHHVADNIPIKPIDYLNKANYTEVIERGHGAPELC",
    "response#3": "VRWCVKSEKELKKCHDLAANVAGFSCVRRDDSLECIQAIKREEADAITLDGGDIYIAGLHNYNLQPIIAEDYGEDSDTCYYAVAVAKKGTEFGFLDLRGKKSCHTGLGKSAGWNIPIGTLVTVGQIQWAGIEDRPVESAVSDFFNASCAPGANRDSQLCQLCMGDCSRSHNEPYYDYSGAFQCLKDGAGEVAFIKHLTVPAAEKASYELLCKDNTRAPIDSYKTCHLARVPAHAVVSRKDPRLANLIYSKLMAVTNFNLFSSDGYAAKNLMFKDSTQNLVQLPMTTDSFLYLGAEYMSTIRSLQ"
  },
  {
    "reference": "MGDVVATIKVMPESPDVDLEALKKEIQERIPEGTELHKIDEEPIAFGLVALNVMVVVGDAEGGTEAAEESLSGIEGVSNIEVTDVRRLM",
    "instruction": "Generate a protein sequence optimized for the following function requirements. 1. The designed protein must be able to perform translation elongation factor activity under specific conditions.2. The protein must exhibit the following characteristics: Promotes the exchange of GDP for GTP in EF-1-alpha/GDP, thus allowing the regeneration of EF-1-alpha/GTP that could then be used to form the ternary complex EF-1-alpha/GTP/AAtRNA.. The designed protein sequence is ",
    "response#1": "MGDVLVVFRVLPEDVDNDLSRLESKIREKLNGVCKINDIKTEEIGFGLKALNVQAIMPDEEGMADRVESILAGIEGVSQVDTKDISLK",
    "response#2": "MGEVVATLKIMPESPDVDLEALKAAIQAAMPAEAEFHKIEEEPIAFGLVALNLIFIIEDGEGGTESTEEAMAKLADVASVEITDTRRR",
    "response#3": "MADVLVSFKLLPSDSDVDTSVMESEVKEKLNGVCKINNIEEKDIGFGLKYIHLEVIVEDKEGEVDRIEKVLSMVKGVGEINTENVSLN"
  },
  {
    "reference": "MRVRGIQRNWLQWWMWSILGLWLIIISKGKEDLWVTVYYGVPVWKEAKTTLFCASNAKAYDQEVHNVWATHACVPTDPNPQEILLENVTEYFNMWENDMADQMHEDIISLWDQSLNPCVKLTPLPVAINSTEVVNSTSTNGNSTSNTGNSTSNNGTRINEMRGEIKNCSFNITTEVRGDKQQMYALFYRRDIVATNNDNDSYVLINCNTSTITQACPKVSFDPIPIHYCAPAGYAILKCNNKTFNGTGLCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRIENQADNAKTIIAHLNESVHIVCTRPNNNTRTSVRIGPGQTFYATGDIIGDIRRAHCNISREGWDKTLQKVKGKLKEHFPNKTIEFEPHSGGDLEVTKPSFKCKWGFFLSNPTIVFTNHAQDKTTGGSGNNETIPLPCRIKQIVNMWQVVGPAMYAPLIAGNITSNSSITGLLLTRDGGIVTNATDTETFRPGGGNMKDNWRSELYKYKVVEIKPLGVAPTKAQRKVVKREREKRAVGMGAVLLGFLGAAGSTMGAASITLTVQARQLLSGIVQQQSNLLKAIEAQQHLLQLTVWGIKQLQARVLAIERYLKDQQLLGLWGCSGKLICATAVPWNYTWSNKSYTDIWDNMTWMQWDREIDNYTDTIYRLLEDSQTQQENNEKDLLALDSWKNLWSWFDISNWLWYIKIFIMIVGGLIGLRIIIGVLSIVKRVRQGYSPLSLQTLIPNPREPDRLGGIEEEGGEQGRDRSIRLLSGFLSIAWDDL",
    "instruction": "Synthesize a protein sequence that has the desired solubility or other physical properties for the intended use. 1. The protein contains novel Human immunodeficiency virus 1 envelope glycoprotein Gp120, Retroviral envelope protein GP41-like domains that confer a unique function or activity.2. For general function, the protein need meet that During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain; Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane; The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes; Transmembrane protein gp41: Acts as a class I viral fusion protein; The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes; Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state3. The Cytoplasmic topological domain of the protein should be designed to be flexible or rigid.4. A protein that can perform structural molecule activity in host cell plasma membrane, viral envelope, plasma membrane to support positive regulation of receptor clustering, virion attachment to host cell, positive regulation of plasma membrane raft polarization. The designed protein sequence is ",
    "response#1": "MRVKGIKRNYQPLWRWGTMLLGMLMICSAAEKLWVTVYYGVPAWRDADTTLFCASDAKAHSTEVHNIWATHACVPTDPNPQEIYLENVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTSANLTKVNNATTSNDFNPIIGNITDEVKNCSFNMTTELRDKKHKVYALFYSLDIVQIDNSSEYRLIKCNTSVIKQACPKISFDPIPIHYCTPAGYAILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENITNNVKTIIVHLNKSVEINCTRPSNNTRTSIAIGPGQLFYRTGDIIGDIRAAYCEINGTKWNKVLKQVTEKLKEHFKEKNITFQQPSGGDLEVITHHFNCRGEFFYCNTTRLFNNTCINNETIEGCQNITIPCKIKQIINMWQETGQAMYAPPIRGKISCISNITGILLTRDGGANKTNEEIFRPGGGNIRDNWRSELYKYKVVQIEPLGVAPTRAKRRVVEREKRAVGLGAVFLGFLGAAGSTMGAASVTLTVQARLLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVLALERYLRDQQLLGIWGCSGKLICTTNVPWNSSWSNRSQNEIWDKMTWIQWEREIDNYTQGIYNLLEESQNQQEKNEKELLELDKWASLWNWFDITNWLWYIKIFIMIVGGLIGLKIIFTVLSIVNRVRQGYSPLSFQTRLPAQRGPDRPEGIEEEGGE",
    "response#2": "MRVMGIRKNCQHLLIWSGIMLLGMLMICSATENLWVTVYYGVPVWKEAKTTLFCASDAKAYDKEVHNVWATHACVPTDPSPQEMFLANVTENFNMWENDMVDQMHQDIISLWDESLKPCVKLTPLCVTLNCRNANLNSTRKSLWVTVYYGVPAWRDADTTLFCASDAKAHSTEVHNIWATHACVPTDPNPQEIYLENVTENFNMWKNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTSANLTKVNNATTSNDFNPIIGNITDEVKNCSFNMTTELRDKKHKVYALFYSLDIVQIDNSSEYRLIKCNTSVIKQACPKISFDPIPIHYCTPAGYAILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSENITNNVKTIIVHLNKSVEINCTRPSNNTRTSIAIGPGQLFYRTGDIIGDIRAAYCEINGTKWNKVLKQVTEKLKEHFKEKNITFQQPSGGDLEVITHHFNCRGEFFYCNTTRLFNNTCINNETIEGCQNITIPCKIKQIINMWQETGQAMYAPPIRGKISCISNITGILLTRDGGANKTNEEIFRPGGGNIRDNWRSELYKYKVVQIEPLGVAPTRAKRRVVEREKRAIGMGAVFLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQARVLAIERYLKDQQLLGMWGCSGKLICTTAVPWNSSWSNKSQNEIWGNMTWMQWDREINNYTNTIYRLLEDSQNQQEKNEKDLLALDSWKNLWNWFDISKWLWYIKIFIMIIGGLIGLRIIFAVLSIVKRVRQGYSPLSFQT",
    "response#3": "MRVMETRKNYQHLWRGGILLLGLLMICSAEEKLWVTIYYGVPVWKEATTTLFCASDAKAYSTEKHNVWATHACVPTDPNPQEVVLENVTENFNMWKNNMVEQMHEDIIGLWDQSLKPCVKLTPLCVTLNCTNYERNGKNTTRQDNTTEMEMLWVTVYYGVPVWKDAKTTLFCASDAKAYETEVHNVWATHACVPTDPNPQEMVLKNVTENFNMWKNDMVDQMHEDVISLWDESLKPCVKLTPLCVTLNCSNYNSNGTHNVSQNEPYNESEREIKNCTFNTTTEVNDKKEKVRALFYRLDIVPLNEKNSSNSSTSESYRLINCNTSAMTQACPKVSFDPIPIHYCTPAGYAILKCNDKKFNGTGPCHNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIISSENLTNNAKTIIVHLNESVEIVCTRPNNNTRKSIRIGPGQTFYATGDIIGDIRQAHCNISRSNWTKTLQKVKGKLEKHFPNKTLIFNSSSGGDLEITTHSFNCRGEFFYCNTSGLFNDTMLNDTESNSSIITIPCRIKQIINMWQEVGRAMYAPPIAGNITCKSNITGILLVHDGGEPNKTEDTFRPGGGDMRDNWRSELYKYKVVEIKPLGVAPTEAKRRVVQREKRAVGLGAVFLGFLGAAGAAGTMGAASLTLTVQARQLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLALERYLRDQQLLGIWGCSGKLICTTTVPWNSSWSNKTYNSIWDNMTWLQWDREISNYTELIYSLIEESQNQQEKNEKELLALDKWASLWNWFDITSWLWYIRIFIMIIGGLIGLRIVFAVLNIINRVRQGYSPLSFQTLTH"
  },
  {
    "reference": "MKLSWKTFLLWSLPIMIISFFLWQGFLTPTSSDLNSNIANSRMTYGRFLEYLDMGWVKKVDIYDNGHTAIVEAVGPELGNRLQKIRVELPTSAPELITKLKKNNIDLDAHPTKNNTAIWNLIGNLFFPLLLIAGLAYLFRRSNNTNGGPGQAMSFGKSKALFQMEAKTGVIFDDVAGIDEAKEEFEEVVTFLKQPESFTAVGAKIPKGVLLVGPPGTGKTLLAKAIAGEANVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVFIDEIDAVGRQRGTGIGGGNDEREQTLNQLLTEMDGFEGNTGIIVIAATNRADILDSALLRPGRFDRQVNVDVPDFKGRLAILNVHARNKKIAPTVSLSIIARRTPGFSGADLANLLNEAAILTVRRKKNAITLNEIDTSIDRIIAGMEGMPLIDSKSKRLIAYHEIGHAIVGTLLKNHYPVQKVTLIPRGQARGLTWFTPSEDQNLISRSEIKARIMGALGGRAAEEIVFGDSEVTTGASNDLQQITSMARQMVTRFGMSSIGPLSLENEESNPFLGRGMVSSSEYSDEIAIKIDEQIQKIVQECHQNTLKIIQDNRVVIDRLVDLLIEKETIDGDEFREIISEYTSIPQKYEYAK",
    "instruction": "Develop a protein sequence with increased stability under specific condition. 1. The protein should contain a Helical membrane-spanning region.2. The presence of Zn(2+) is necessary for the protein to function in the desired environment.3. The ideal protein would be one that plays a critical role in proteolysis, cell division through ATP hydrolysis activity, ATP-dependent peptidase activity within plasma membrane, chloroplast. The designed protein sequence is ",
    "response#1": "VLLVGPPGTGKTLLARAIAGEANVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLNQLLVEMDGFEPNESIILIAATNRPDVLDPALLRPGRFDRQVVVAF",
    "response#2": "VLLVGPPGTGKTLLARAVAGEAKVPFFSISGSEFVEMFVGVGAARVRDLFEQARAQAPAIIFIDELDALGRARGVGPLGGGHDEKEQTLNQLLVELDGFDTSTGLVLLAATNRPEILDPALLRAGRFDRQVLVL",
    "response#3": "VLLVGPPGTGKTLLAKAIAGEAGVPFFSLSGSEFVEMFVGVGASRVRDLFKKAKENAPCIVFIDEIDAVGRQRGAGIGGGNDEREQTLNQLLTEMDGFEGNSGVIVIAATNRPDVLDAALLRPGRFDRQITVLFSVDLKGIARRTPGFTGADLANLLNEGALLAARRNKKRIGIEEN"
  }
]